doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text	PSource	NProtein	NID	SiteSource	SiteName
29391424	4	0	theme	Automated	493:501	arg1	annotation					503:512	Automated annotation	493:512	Automated annotation of both N- and O-linked glycopeptides	493:550	Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.					
29391424	3	1	dep	letter	374:379	arg1	codes					381:385	codes	381:385	codes	381:385	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	4	2	gly	glycopeptides	538:550	arg2	glycopeptides					538:550	O-linked glycopeptides	529:550	O-linked glycopeptides	529:550	Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.					
29391424	1	3	theme	glycoproteomics	164:178	arg1	field					155:159	the field	151:159	the field of glycoproteomics	151:178	Workflows capable of determining glycopeptides in large-scale are missing in the field of glycoproteomics.					
29391424	5	4	theme	glycopeptide	720:731	arg1	variants					733:740	3447 non-redundant N-linked glycopeptide variants	692:740	3447 non-redundant N-linked glycopeptide variants	692:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	5	5	theme	large-scale	598:608	arg1	study					610:614	a large-scale study	596:614	a large-scale study	596:614	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	5	6	theme	N-linked	711:718	arg1	variants					733:740	3447 non-redundant N-linked glycopeptide variants	692:740	3447 non-redundant N-linked glycopeptide variants	692:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	1	7	gly	glycopeptides	107:119	arg2	glycopeptides					107:119	glycopeptides	107:119	glycopeptides in large-scale	107:134	Workflows capable of determining glycopeptides in large-scale are missing in the field of glycoproteomics.					
29391424	5	8	theme	unique	661:666	arg1	sites					682:686	970 unique glycosylation sites	657:686	970 unique glycosylation sites	657:686	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	0	9	gly	glycopeptide	19:30	arg2	glycopeptide					19:30	Large-scale intact glycopeptide identification	0:45	Large-scale intact glycopeptide identification by Mascot database search.	0:72	Large-scale intact glycopeptide identification by Mascot database search.					
29391424	6	10	dep	matching	904:911	arg1	ii					900:901	ii	900:901	ii	900:901	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	5	11	theme	glycosylation	668:680	arg1	sites					682:686	970 unique glycosylation sites	657:686	970 unique glycosylation sites	657:686	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	1	12	from	field	155:159	arg1	missing					140:146	missing	140:146	missing	140:146	Workflows capable of determining glycopeptides in large-scale are missing in the field of glycoproteomics.					
29391424	5	13	link	N-linked	711:718	arg1	variants					733:740	3447 non-redundant N-linked glycopeptide variants	692:740	3447 non-redundant N-linked glycopeptide variants	692:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	6	14	theme	batch-wise	982:991	arg1	analysis					993:1000	(iii) high-throughput, batch-wise analysis	959:1000	analysis	993:1000	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	4	15	link	O-linked	529:536	arg1	glycopeptides					538:550	O-linked glycopeptides	529:550	O-linked glycopeptides	529:550	Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.					
29391424	6	16	theme	sets	1038:1041	arg1	analysis					993:1000	(iii) high-throughput, batch-wise analysis	959:1000	analysis	993:1000	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	6	16	theme	sets	1038:1041	arg1	elucidation					846:856	the (i) elucidation	838:856	the (i) elucidation of N- and O-linked glycopeptide spectra	838:896	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	1	17	theme	capable	84:90	arg1	Workflows					74:82	Workflows	74:82	Workflows capable of determining glycopeptides in large-scale	74:134	Workflows capable of determining glycopeptides in large-scale are missing in the field of glycoproteomics.					
29391424	0	18	theme	database	57:64	arg1	search					66:71	Mascot database search	50:71	Mascot database search	50:71	Large-scale intact glycopeptide identification by Mascot database search.					
29391424	6	19	theme	glycopeptide	877:888	arg1	spectra					890:896	O-linked glycopeptide spectra	868:896	O-linked glycopeptide spectra	868:896	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	4	20	gly	glycoproteins	578:590	arg1	glycoproteins					578:590	standard glycoproteins	569:590	standard glycoproteins	569:590	Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.					
29391424	0	21	theme	intact	12:17	arg1	identification					32:45	Large-scale intact glycopeptide identification	0:45	Large-scale intact glycopeptide identification by Mascot database search.	0:72	Large-scale intact glycopeptide identification by Mascot database search.					
29391424	6	22	theme	N-	861:862	arg1	analysis					993:1000	(iii) high-throughput, batch-wise analysis	959:1000	analysis	993:1000	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	6	22	theme	N-	861:862	arg1	elucidation					846:856	the (i) elucidation	838:856	the (i) elucidation of N- and O-linked glycopeptide spectra	838:896	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	5	23	theme	257	628:630	arg1	glycoproteins					632:644	257 glycoproteins	628:644	257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants	628:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	6	24	theme	spectra	890:896	arg1	analysis					993:1000	(iii) high-throughput, batch-wise analysis	959:1000	analysis	993:1000	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	6	24	theme	spectra	890:896	arg1	elucidation					846:856	the (i) elucidation	838:856	the (i) elucidation of N- and O-linked glycopeptide spectra	838:896	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	5	25	gly	glycosylation	668:680	arg2	sites					682:686	970 unique glycosylation sites	657:686	970 unique glycosylation sites	657:686	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	5	25	gly	glycosylation	668:680	arg2	970					657:659	970	657:659	970	657:659	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	0	26	theme	Large-scale	0:10	arg1	identification					32:45	Large-scale intact glycopeptide identification	0:45	Large-scale intact glycopeptide identification by Mascot database search.	0:72	Large-scale intact glycopeptide identification by Mascot database search.					
29391424	5	27	theme	glycoproteins	632:644	arg1	total					619:623	a total	617:623	a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants	617:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	6	28	theme	single	787:792	arg1	tool					794:797	a single tool	785:797	a single tool	785:797	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	3	29	theme	glycan	425:430	arg1	sequences					432:440	glycan sequences	425:440	glycan sequences	425:440	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	3	30	theme	Mascot	292:297	arg1	engine					306:311	the Mascot search engine	288:311	the Mascot search engine	288:311	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	1	31	from	missing	140:146	arg1	field					155:159	the field	151:159	the field of glycoproteomics	151:178	Workflows capable of determining glycopeptides in large-scale are missing in the field of glycoproteomics.					
29391424	3	32	gly	glycoprotein	469:480	arg1	glycoprotein					469:480	custom glycoprotein databases	462:490	custom glycoprotein databases	462:490	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	3	33	dep	linearizing	413:423	arg1	ii					409:410	ii	409:410	ii	409:410	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	0	34	theme	glycopeptide	19:30	arg1	identification					32:45	Large-scale intact glycopeptide identification	0:45	Large-scale intact glycopeptide identification by Mascot database search.	0:72	Large-scale intact glycopeptide identification by Mascot database search.					
29391424	4	35	theme	standard	569:576	arg1	glycoproteins					578:590	standard glycoproteins	569:590	standard glycoproteins	569:590	Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.					
29391424	3	36	theme	search	299:304	arg1	engine					306:311	the Mascot search engine	288:311	the Mascot search engine	288:311	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	6	37	link	O-linked	868:875	arg1	spectra					890:896	O-linked glycopeptide spectra	868:896	O-linked glycopeptide spectra	868:896	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	0	38	theme	Mascot	50:55	arg1	search					66:71	Mascot database search	50:71	Mascot database search	50:71	Large-scale intact glycopeptide identification by Mascot database search.					
29391424	6	39	theme	data	1033:1036	arg1	sets					1038:1041	large-scale glycoproteomics data sets	1005:1041	large-scale glycoproteomics data sets	1005:1041	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	2	40	theme	spectra	257:263	arg1	annotation					218:227	automated annotation	208:227	automated annotation of intact glycopeptide mass spectra	208:263	We present an approach for automated annotation of intact glycopeptide mass spectra.					
29391424	6	41	gly	glycopeptide	877:888	arg2	glycopeptide					877:888	O-linked glycopeptide spectra	868:896	O-linked glycopeptide spectra	868:896	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	5	42	gly	glycoproteins	632:644	arg1	glycoproteins					632:644	257 glycoproteins	628:644	257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants	628:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	6	43	theme	protein	936:942	arg1	sequences					944:952	known protein sequences	930:952	known protein sequences	930:952	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	6	44	theme	high-throughput	965:979	arg1	analysis					993:1000	(iii) high-throughput, batch-wise analysis	959:1000	analysis	993:1000	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	2	45	theme	mass	252:255	arg1	spectra					257:263	intact glycopeptide mass spectra	232:263	intact glycopeptide mass spectra	232:263	We present an approach for automated annotation of intact glycopeptide mass spectra.					
29391424	5	46	theme	serum	764:768	arg1	samples					770:776	24 serum samples	761:776	24 serum samples	761:776	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	4	47	theme	N-	522:523	arg1	annotation					503:512	Automated annotation	493:512	Automated annotation of both N- and O-linked glycopeptides	493:550	Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.					
29391424	3	48	theme	intact	317:322	arg1	analysis					337:344	intact glycopeptide analysis	317:344	intact glycopeptide analysis	317:344	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	2	49	theme	glycopeptide	239:250	arg1	spectra					257:263	intact glycopeptide mass spectra	232:263	intact glycopeptide mass spectra	232:263	We present an approach for automated annotation of intact glycopeptide mass spectra.					
29391424	5	50	gly	glycopeptide	720:731	arg2	glycopeptide					720:731	3447 non-redundant N-linked glycopeptide variants	692:740	3447 non-redundant N-linked glycopeptide variants	692:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	2	51	gly	glycopeptide	239:250	arg2	glycopeptide					239:250	intact glycopeptide mass spectra	232:263	intact glycopeptide mass spectra	232:263	We present an approach for automated annotation of intact glycopeptide mass spectra.					
29391424	4	52	theme	glycopeptides	538:550	arg1	annotation					503:512	Automated annotation	493:512	Automated annotation of both N- and O-linked glycopeptides	493:550	Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.					
29391424	3	53	theme	glycopeptide	324:335	arg1	analysis					337:344	intact glycopeptide analysis	317:344	intact glycopeptide analysis	317:344	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	2	54	theme	intact	232:237	arg1	spectra					257:263	intact glycopeptide mass spectra	232:263	intact glycopeptide mass spectra	232:263	We present an approach for automated annotation of intact glycopeptide mass spectra.					
29391424	6	55	theme	O-linked	868:875	arg1	spectra					890:896	O-linked glycopeptide spectra	868:896	O-linked glycopeptide spectra	868:896	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	6	56	dep	elucidation	846:856	arg1	i					843:843	i	843:843	i	843:843	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	4	57	theme	O-linked	529:536	arg1	glycopeptides					538:550	O-linked glycopeptides	529:550	O-linked glycopeptides	529:550	Automated annotation of both N- and O-linked glycopeptides was proven using standard glycoproteins.					
29391424	3	58	dep	assigning	360:368	arg1	i					357:357	i	357:357	i	357:357	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	6	59	gly	glycopeptides	913:925	arg2	glycopeptides					913:925	glycopeptides	913:925	glycopeptides	913:925	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	3	60	theme	custom	462:467	arg1	databases					482:490	custom glycoprotein databases	462:490	custom glycoprotein databases	462:490	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	6	61	theme	large-scale	1005:1015	arg1	sets					1038:1041	large-scale glycoproteomics data sets	1005:1041	large-scale glycoproteomics data sets	1005:1041	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	5	62	theme	non-redundant	697:709	arg1	variants					733:740	3447 non-redundant N-linked glycopeptide variants	692:740	3447 non-redundant N-linked glycopeptide variants	692:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	2	63	theme	automated	208:216	arg1	annotation					218:227	automated annotation	208:227	automated annotation of intact glycopeptide mass spectra	208:263	We present an approach for automated annotation of intact glycopeptide mass spectra.					
29391424	3	64	theme	glycoprotein	469:480	arg1	databases					482:490	custom glycoprotein databases	462:490	custom glycoprotein databases	462:490	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	3	65	gly	glycopeptide	324:335	arg2	glycopeptide					324:335	intact glycopeptide analysis	317:344	intact glycopeptide analysis	317:344	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	6	66	theme	glycoproteomics	1017:1031	arg1	sets					1038:1041	large-scale glycoproteomics data sets	1005:1041	large-scale glycoproteomics data sets	1005:1041	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	1	67	from	glycopeptides	107:119	arg1	large-scale					124:134	large-scale	124:134	large-scale	124:134	Workflows capable of determining glycopeptides in large-scale are missing in the field of glycoproteomics.					
29391424	3	68	dep	preparing	452:460	arg1	iii					447:449	iii	447:449	iii	447:449	The steps in adopting the Mascot search engine for intact glycopeptide analysis included: (i) assigning one letter codes for monosaccharides, (ii) linearizing glycan sequences and (iii) preparing custom glycoprotein databases.					
29391424	6	69	theme	known	930:934	arg1	sequences					944:952	known protein sequences	930:952	known protein sequences	930:952	Thus, a single tool was developed that collectively allows the (i) elucidation of N- and O-linked glycopeptide spectra, (ii) matching glycopeptides to known protein sequences, and (iii) high-throughput, batch-wise analysis of large-scale glycoproteomics data sets.					
29391424	5	70	contain	containing	646:655	arg2	sites					682:686	970 unique glycosylation sites	657:686	970 unique glycosylation sites	657:686	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	5	70	contain	containing	646:655	arg2	variants					733:740	3447 non-redundant N-linked glycopeptide variants	692:740	3447 non-redundant N-linked glycopeptide variants	692:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
29391424	5	70	contain	containing	646:655	arg1	glycoproteins					632:644	257 glycoproteins	628:644	257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants	628:740	In a large-scale study, a total of 257 glycoproteins containing 970 unique glycosylation sites and 3447 non-redundant N-linked glycopeptide variants were identified in 24 serum samples.					
30903686	0	0	theme	mouse	72:76	arg1	proteins					78:85	human and mouse proteins	62:85	human and mouse proteins	62:85	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	4	1	theme	mouse	912:916	arg1	sites					932:936	human and mouse glycosylation sites	902:936	human and mouse glycosylation sites	902:936	RESULTS: The method, called SPRINT-Gly, achieved consistent results between ten-fold cross validation and independent test for predicting human and mouse glycosylation sites.					
30903686	1	2	theme	important	251:259	arg1	role					261:264	an important role	248:264	an important role	248:264	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30903686	2	3	gly	glycosylation	456:468	arg2	sites					470:474	glycosylation sites	456:474	glycosylation sites	456:474	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	9	4	dep	INFORMATION	1479:1489	arg1	available					1515:1523	available	1515:1523	available	1515:1523	SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.					
30903686	1	5	theme	Protein	154:160	arg1	glycosylation					162:174	MOTIVATION: Protein glycosylation	142:174	MOTIVATION: Protein glycosylation	142:174	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30903686	9	6	theme	Supplementary	1492:1504	arg1	data					1506:1509	Supplementary data	1492:1509	Supplementary data	1492:1509	SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.					
30903686	3	7	theme	N-/O-linked	717:727	arg1	sites					743:747	N-/O-linked glycosylation sites	717:747	N-/O-linked glycosylation sites	717:747	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	5	8	dep	model	976:980	arg1	performs					982:989	performs	982:989	performs equally well in human glycoproteins and vice versa	982:1040	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	0	9	theme	proteins	78:85	arg1	N-					23:24	N-	23:24	N-	23:24	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	0	9	theme	proteins	78:85	arg1	glycosylation					39:51	O-linked glycosylation	30:51	O-linked glycosylation	30:51	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	6	10	theme	Matthews	1178:1185	arg1	coefficient					1199:1209	Matthews correlation coefficient	1178:1209	Matthews correlation coefficient	1178:1209	Overall, SPRINT-Gly is 18% and 50% higher in Matthews correlation coefficient than the next best method compared in N-linked and O-linked sites, respectively.					
30903686	2	11	gly	glycopeptides	430:442	arg2	glycopeptides					430:442	glycopeptides	430:442	glycopeptides identifying glycosylation sites	430:474	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	5	12	dep	performs	982:989	arg1	glycoproteins					1013:1025	human glycoproteins	1007:1025	human glycoproteins	1007:1025	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	5	12	dep	performs	982:989	arg1	versa					1036:1040	versa	1036:1040	versa	1036:1040	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	1	13	theme	immune	269:274	arg1	responses					276:284	immune responses	269:284	immune responses	269:284	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30903686	5	14	link	O-linked	1086:1093	arg1	sites					1095:1099	O-linked sites	1086:1099	O-linked sites	1086:1099	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	2	15	theme	computational	525:537	arg1	methods					539:545	computational methods	525:545	computational methods	525:545	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	2	16	gly	microheterogeneity	408:425	arg1	glycopeptides					430:442	glycopeptides	430:442	glycopeptides identifying glycosylation sites	430:474	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	4	17	theme	consistent	813:822	arg1	results					824:830	consistent results	813:830	consistent results between ten-fold cross validation and independent test for predicting human and mouse glycosylation sites	813:936	RESULTS: The method, called SPRINT-Gly, achieved consistent results between ten-fold cross validation and independent test for predicting human and mouse glycosylation sites.					
30903686	2	18	theme	challenging	481:491	arg1	task					493:496	a challenging task	479:496	a challenging task	479:496	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	6	19	link	O-linked	1262:1269	arg1	sites					1271:1275	N-linked and O-linked sites	1249:1275	N-linked and O-linked sites	1249:1275	Overall, SPRINT-Gly is 18% and 50% higher in Matthews correlation coefficient than the next best method compared in N-linked and O-linked sites, respectively.					
30903686	4	20	theme	human	902:906	arg1	sites					932:936	human and mouse glycosylation sites	902:936	human and mouse glycosylation sites	902:936	RESULTS: The method, called SPRINT-Gly, achieved consistent results between ten-fold cross validation and independent test for predicting human and mouse glycosylation sites.					
30903686	6	21	theme	correlation	1187:1197	arg1	coefficient					1199:1209	Matthews correlation coefficient	1178:1209	Matthews correlation coefficient	1178:1209	Overall, SPRINT-Gly is 18% and 50% higher in Matthews correlation coefficient than the next best method compared in N-linked and O-linked sites, respectively.					
30903686	4	22	gly	glycosylation	918:930	arg2	sites					932:936	human and mouse glycosylation sites	902:936	human and mouse glycosylation sites	902:936	RESULTS: The method, called SPRINT-Gly, achieved consistent results between ten-fold cross validation and independent test for predicting human and mouse glycosylation sites.					
30903686	7	23	theme	novel	1345:1349	arg1	structure					1351:1359	novel structure	1345:1359	novel structure	1345:1359	This improved performance is due to the inclusion of novel structure and sequence-based features.					
30903686	0	24	link	O-linked	30:37	arg1	glycosylation					39:51	O-linked glycosylation	30:51	O-linked glycosylation	30:51	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	3	25	theme	deep	637:640	arg1	networks					658:665	deep learning neural networks	637:665	deep learning neural networks	637:665	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	5	26	from	differences	1071:1081	arg1	sites					1095:1099	O-linked sites	1086:1099	O-linked sites	1086:1099	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	2	27	theme	ionization	382:391	arg1	efficiency					393:402	poor ionization efficiency	377:402	poor ionization efficiency	377:402	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	4	28	theme	ten-fold	840:847	arg1	validation					855:864	ten-fold cross validation	840:864	ten-fold cross validation	840:864	RESULTS: The method, called SPRINT-Gly, achieved consistent results between ten-fold cross validation and independent test for predicting human and mouse glycosylation sites.					
30903686	0	29	theme	predicted	109:117	arg1	properties					130:139	predicted structural properties	109:139	predicted structural properties	109:139	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	6	30	theme	N-linked	1249:1256	arg1	sites					1271:1275	N-linked and O-linked sites	1249:1275	N-linked and O-linked sites	1249:1275	Overall, SPRINT-Gly is 18% and 50% higher in Matthews correlation coefficient than the next best method compared in N-linked and O-linked sites, respectively.					
30903686	3	31	theme	largest	573:579	arg1	dataset					581:587	the largest dataset	569:587	the largest dataset of human and mouse glycosylation sites	569:626	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	3	32	link	N-/O-linked	717:727	arg1	sites					743:747	N-/O-linked glycosylation sites	717:747	N-/O-linked glycosylation sites	717:747	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	2	33	theme	glycosylation	456:468	arg1	sites					470:474	glycosylation sites	456:474	glycosylation sites	456:474	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	3	34	theme	human	592:596	arg1	sites					622:626	human and mouse glycosylation sites	592:626	human and mouse glycosylation sites	592:626	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	1	35	theme	intercellular	287:299	arg1	signaling					301:309	intercellular signaling	287:309	intercellular signaling	287:309	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30903686	6	36	theme	next	1220:1223	arg1	method					1230:1235	the next best method	1216:1235	the next best method compared in N-linked and O-linked sites, respectively	1216:1289	Overall, SPRINT-Gly is 18% and 50% higher in Matthews correlation coefficient than the next best method compared in N-linked and O-linked sites, respectively.					
30903686	5	37	theme	human	1007:1011	arg1	glycoproteins					1013:1025	human glycoproteins	1007:1025	human glycoproteins	1007:1025	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	3	38	gly	glycosylation	608:620	arg2	sites					622:626	human and mouse glycosylation sites	592:626	human and mouse glycosylation sites	592:626	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	0	39	theme	O-linked	30:37	arg1	glycosylation					39:51	O-linked glycosylation	30:51	O-linked glycosylation	30:51	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	3	40	theme	support	671:677	arg1	machine					686:692	support vector machine	671:692	support vector machine classifiers	671:704	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	2	41	theme	glycopeptides	430:442	arg1	microheterogeneity					408:425	microheterogeneity	408:425	microheterogeneity	408:425	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	2	41	theme	glycopeptides	430:442	arg1	efficiency					393:402	poor ionization efficiency	377:402	poor ionization efficiency	377:402	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	3	42	theme	glycosylation	608:620	arg1	sites					622:626	human and mouse glycosylation sites	592:626	human and mouse glycosylation sites	592:626	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	6	43	theme	best	1225:1228	arg1	method					1230:1235	the next best method	1216:1235	the next best method compared in N-linked and O-linked sites, respectively	1216:1289	Overall, SPRINT-Gly is 18% and 50% higher in Matthews correlation coefficient than the next best method compared in N-linked and O-linked sites, respectively.					
30903686	1	44	theme	abundant	195:202	arg1	modifications					223:235	the most abundant post-translational modifications	186:235	the most abundant post-translational modifications	186:235	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30903686	0	45	theme	structural	119:128	arg1	properties					130:139	predicted structural properties	109:139	predicted structural properties	109:139	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	3	46	theme	neural	651:656	arg1	networks					658:665	deep learning neural networks	637:665	deep learning neural networks	637:665	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	3	47	theme	sites	622:626	arg1	dataset					581:587	the largest dataset	569:587	the largest dataset of human and mouse glycosylation sites	569:626	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	1	48	theme	post-translational	204:221	arg1	modifications					223:235	the most abundant post-translational modifications	186:235	the most abundant post-translational modifications	186:235	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30903686	8	49	dep	//sparks-lab.org/server/SPRINT-Gly/	1428:1462	arg1	AVAILABILITY					1390:1401	AVAILABILITY	1390:1401	AVAILABILITY	1390:1401	AVAILABILITY AND IMPLEMENTATION: http://sparks-lab.org/server/SPRINT-Gly/.					
30903686	8	49	dep	//sparks-lab.org/server/SPRINT-Gly/	1428:1462	arg1	http					1423:1426	http	1423:1426	http	1423:1426	AVAILABILITY AND IMPLEMENTATION: http://sparks-lab.org/server/SPRINT-Gly/.					
30903686	8	49	dep	//sparks-lab.org/server/SPRINT-Gly/	1428:1462	arg1	IMPLEMENTATION					1407:1420	IMPLEMENTATION	1407:1420	IMPLEMENTATION	1407:1420	AVAILABILITY AND IMPLEMENTATION: http://sparks-lab.org/server/SPRINT-Gly/.					
30903686	3	50	gly	glycosylation	729:741	arg2	sites					743:747	N-/O-linked glycosylation sites	717:747	N-/O-linked glycosylation sites	717:747	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	7	51	theme	features	1380:1387	arg1	inclusion					1332:1340	the inclusion	1328:1340	the inclusion of novel structure and sequence-based features	1328:1387	This improved performance is due to the inclusion of novel structure and sequence-based features.					
30903686	6	52	link	N-linked	1249:1256	arg1	sites					1271:1275	N-linked and O-linked sites	1249:1275	N-linked and O-linked sites	1249:1275	Overall, SPRINT-Gly is 18% and 50% higher in Matthews correlation coefficient than the next best method compared in N-linked and O-linked sites, respectively.					
30903686	4	53	theme	independent	870:880	arg1	test					882:885	independent test	870:885	independent test	870:885	RESULTS: The method, called SPRINT-Gly, achieved consistent results between ten-fold cross validation and independent test for predicting human and mouse glycosylation sites.					
30903686	6	54	theme	O-linked	1262:1269	arg1	sites					1271:1275	N-linked and O-linked sites	1249:1275	N-linked and O-linked sites	1249:1275	Overall, SPRINT-Gly is 18% and 50% higher in Matthews correlation coefficient than the next best method compared in N-linked and O-linked sites, respectively.					
30903686	7	55	theme	structure	1351:1359	arg1	inclusion					1332:1340	the inclusion	1328:1340	the inclusion of novel structure and sequence-based features	1328:1387	This improved performance is due to the inclusion of novel structure and sequence-based features.					
30903686	5	56	theme	O-linked	1086:1093	arg1	sites					1095:1099	O-linked sites	1086:1099	O-linked sites	1086:1099	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	3	57	theme	mouse	602:606	arg1	sites					622:626	human and mouse glycosylation sites	592:626	human and mouse glycosylation sites	592:626	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	5	58	theme	mouse-trained	962:974	arg1	model					976:980	a mouse-trained model	960:980	a mouse-trained model performs equally well in human glycoproteins and vice versa	960:1040	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	1	59	theme	host-pathogen	329:341	arg1	interactions					343:354	host-pathogen interactions	329:354	host-pathogen interactions	329:354	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30903686	5	60	theme	significant	1059:1069	arg1	differences					1071:1081	significant differences	1059:1081	significant differences in O-linked sites	1059:1099	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	2	61	dep	efficiency	393:402	arg1	the					373:375	the	373:375	the	373:375	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	0	62	dep	N-	23:24	arg1	sites					53:57	sites	53:57	sites	53:57	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	4	63	theme	cross	849:853	arg1	validation					855:864	ten-fold cross validation	840:864	ten-fold cross validation	840:864	RESULTS: The method, called SPRINT-Gly, achieved consistent results between ten-fold cross validation and independent test for predicting human and mouse glycosylation sites.					
30903686	7	64	theme	improved	1297:1304	arg1	performance					1306:1316	This improved performance	1292:1316	This improved performance	1292:1316	This improved performance is due to the inclusion of novel structure and sequence-based features.					
30903686	1	65	theme	modifications	223:235	arg1	one					179:181	one	179:181	one	179:181	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30903686	1	65	theme	modifications	223:235	arg1	modifications					223:235	the most abundant post-translational modifications	186:235	the most abundant post-translational modifications	186:235	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30903686	3	66	theme	vector	679:684	arg1	machine					686:692	support vector machine	671:692	support vector machine classifiers	671:704	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	3	67	theme	learning	642:649	arg1	networks					658:665	deep learning neural networks	637:665	deep learning neural networks	637:665	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	9	68	theme	Bioinformatics	1528:1541	arg1	online					1543:1548	Bioinformatics online	1528:1548	Bioinformatics online	1528:1548	SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.					
30903686	4	69	theme	glycosylation	918:930	arg1	sites					932:936	human and mouse glycosylation sites	902:936	human and mouse glycosylation sites	902:936	RESULTS: The method, called SPRINT-Gly, achieved consistent results between ten-fold cross validation and independent test for predicting human and mouse glycosylation sites.					
30903686	5	70	theme	separate	1101:1108	arg1	models					1110:1115	due to significant differences in O-linked sites separate models	1052:1115	due to significant differences in O-linked sites separate models	1052:1115	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	0	71	theme	human	62:66	arg1	proteins					78:85	human and mouse proteins	62:85	human and mouse proteins	62:85	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	3	72	theme	machine	686:692	arg1	classifiers					694:704	support vector machine classifiers	671:704	support vector machine classifiers	671:704	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	0	73	gly	glycosylation	39:51	arg1	proteins					78:85	human and mouse proteins	62:85	human and mouse proteins	62:85	SPRINT-Gly: predicting N- and O-linked glycosylation sites of human and mouse proteins by using sequence and predicted structural properties.					
30903686	9	74	from	online	1543:1548	arg1	available					1515:1523	available	1515:1523	available	1515:1523	SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.					
30903686	2	75	theme	poor	377:380	arg1	efficiency					393:402	poor ionization efficiency	377:402	poor ionization efficiency	377:402	However, due to the poor ionization efficiency and microheterogeneity of glycopeptides identifying glycosylation sites is a challenging task, and there is a demand for computational methods.					
30903686	7	76	theme	sequence-based	1365:1378	arg1	features					1380:1387	sequence-based features	1365:1387	sequence-based features	1365:1387	This improved performance is due to the inclusion of novel structure and sequence-based features.					
30903686	9	77	theme	SUPPLEMENTARY	1465:1477	arg1	INFORMATION					1479:1489	SUPPLEMENTARY INFORMATION	1465:1489	SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.	1465:1549	SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.					
30903686	5	78	gly	glycoproteins	1013:1025	arg1	glycoproteins					1013:1025	human glycoproteins	1007:1025	human glycoproteins	1007:1025	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	3	79	theme	glycosylation	729:741	arg1	sites					743:747	N-/O-linked glycosylation sites	717:747	N-/O-linked glycosylation sites	717:747	Here, we constructed the largest dataset of human and mouse glycosylation sites to train deep learning neural networks and support vector machine classifiers to predict N-/O-linked glycosylation sites, respectively.					
30903686	5	80	theme	due	1052:1054	arg1	models					1110:1115	due to significant differences in O-linked sites separate models	1052:1115	due to significant differences in O-linked sites separate models	1052:1115	For N-glycosylation, a mouse-trained model performs equally well in human glycoproteins and vice versa, however, due to significant differences in O-linked sites separate models were generated.					
30903686	1	81	theme	MOTIVATION	142:151	arg1	glycosylation					162:174	MOTIVATION: Protein glycosylation	142:174	MOTIVATION: Protein glycosylation	142:174	MOTIVATION: Protein glycosylation is one of the most abundant post-translational modifications that plays an important role in immune responses, intercellular signaling, inflammation and host-pathogen interactions.					
30696735	2	0	theme	resistant	439:447	arg1	starch					449:454	resistant starch	439:454	resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control	439:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	1	1	theme	fiber	303:307	arg1	lack					283:286	the lack	279:286	the lack of fermentable fiber in the diet	279:319	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	1	2	from	lack	283:286	arg1	diet					316:319	the diet	312:319	the diet	312:319	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	1	3	theme	fatty	155:159	arg1	butyrate					187:194	butyrate	187:194	butyrate	187:194	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	1	3	theme	fatty	155:159	arg1	SCFAs					168:172	SCFAs	168:172	SCFAs	168:172	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	1	3	theme	fatty	155:159	arg1	acids					161:165	short-chain fatty acids	143:165	short-chain fatty acids (SCFAs)	143:173	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	6	4	theme	fibers	1682:1687	arg1	effect					1654:1659	the butyrogenic effect	1638:1659	the butyrogenic effect of those fermentable fibers in the short term	1638:1705	Bifidobacteria, though proficient at degrading RS and inulin, may not contribute to the butyrogenic effect of those fermentable fibers in the short term.					
30696735	1	5	theme	acids	161:165	arg1	Production					129:138	Production	129:138	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome	129:217	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	4	6	theme	species	1001:1007	arg1	populations					963:973	populations	963:973	populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities	963:1089	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	2	7	theme	chicory	524:530	arg1	root					532:535	chicory root	524:535	chicory root	524:535	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	6	8	from	effect	1654:1659	arg1	term					1702:1705	the short term	1692:1705	the short term	1692:1705	Bifidobacteria, though proficient at degrading RS and inulin, may not contribute to the butyrogenic effect of those fermentable fibers in the short term.					
30696735	3	9	theme	greatest	596:603	arg1	increase					605:612	the greatest increase	592:612	the greatest increase in total SCFAs, including butyrate	592:647	RPS resulted in the greatest increase in total SCFAs, including butyrate.					
30696735	2	10	theme	starch	560:565	arg1	control					567:573	an accessible corn starch control	541:573	an accessible corn starch control	541:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	4	11	theme	butyrate-producing	982:999	arg1	species					1001:1007	the butyrate-producing species	978:1007	the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities	978:1089	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	1	12	theme	optimal	235:241	arg1	health					243:248	optimal health	235:248	optimal health	235:248	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	0	13	theme	Dietary	76:82	arg1	Interventions					84:96	Dietary Interventions	76:96	Dietary Interventions with Three Fermentable Fibers	76:126	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	6	14	theme	short	1696:1700	arg1	term					1702:1705	the short term	1692:1705	the short term	1692:1705	Bifidobacteria, though proficient at degrading RS and inulin, may not contribute to the butyrogenic effect of those fermentable fibers in the short term.					
30696735	2	15	theme	corn	555:558	arg1	control					567:573	an accessible corn starch control	541:573	an accessible corn starch control	541:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	4	16	theme	fecal	1073:1077	arg1	communities					1079:1089	fecal communities	1073:1089	fecal communities	1073:1089	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	5	17	theme	butyrate	1515:1522	arg1	production					1524:1533	enhanced butyrate production	1506:1533	enhanced butyrate production in response to RS	1506:1551	In particular, R. bromii or C. chartatabidum may be required for enhanced butyrate production in response to RS.					
30696735	4	18	theme	SCFA	1264:1267	arg1	production					1269:1278	SCFA production	1264:1278	SCFA production	1264:1278	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	19	theme	bifidobacteria	748:761	arg1	abundance					735:743	the relative abundance	722:743	the relative abundance of bifidobacteria	722:761	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	2	20	theme	accessible	544:553	arg1	control					567:573	an accessible corn starch control	541:573	an accessible corn starch control	541:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	0	21	dep	Interventions	84:96	arg1	Response					64:71	Response	64:71	Response	64:71	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	2	22	theme	adults	415:420	arg1	diets					388:392	the diets	384:392	the diets of 174 healthy young adults for 2 weeks	384:432	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	3	23	theme	total	617:621	arg1	SCFAs					623:627	total SCFAs	617:627	total SCFAs	617:627	RPS resulted in the greatest increase in total SCFAs, including butyrate.					
30696735	3	23	theme	total	617:621	arg1	butyrate					640:647	butyrate	640:647	butyrate	640:647	RPS resulted in the greatest increase in total SCFAs, including butyrate.					
30696735	4	24	theme	butyrate	1149:1156	arg1	levels.IMPORTANCE					1158:1174	fecal butyrate levels.IMPORTANCE	1143:1174	fecal butyrate levels.IMPORTANCE	1143:1174	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	25	theme	microbiomes	675:685	arg1	majority					663:670	the majority	659:670	the majority of microbiomes	659:685	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	2	26	theme	butyrate	347:354	arg1	production					356:365	butyrate production	347:365	butyrate production	347:365	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	27	theme	young	409:413	arg1	adults					415:420	174 healthy young adults	397:420	174 healthy young adults	397:420	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	6	28	theme	butyrogenic	1642:1652	arg1	effect					1654:1659	the butyrogenic effect	1638:1659	the butyrogenic effect of those fermentable fibers in the short term	1638:1705	Bifidobacteria, though proficient at degrading RS and inulin, may not contribute to the butyrogenic effect of those fermentable fibers in the short term.					
30696735	1	29	from	Production	129:138	arg1	microbiome					208:217	the gut microbiome	200:217	the gut microbiome	200:217	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	2	30	theme	healthy	401:407	arg1	adults					415:420	174 healthy young adults	397:420	174 healthy young adults	397:420	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	31	from	inulin	512:517	arg1	starch					449:454	resistant starch	439:454	resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control	439:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	4	32	from	increase	793:800	arg1	bromii					818:823	Ruminococcus bromii	805:823	Ruminococcus bromii	805:823	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	32	from	increase	793:800	arg1	chartatabidum					840:852	Clostridium chartatabidum	828:852	Clostridium chartatabidum	828:852	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	0	33	with	Interventions	84:96	arg1	Fibers					121:126	Three Fermentable Fibers	103:126	Three Fermentable Fibers	103:126	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	4	34	theme	induced	1044:1050	arg1	changes					1062:1068	inulin induced different changes	1037:1068	inulin induced different changes	1037:1068	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	0	35	theme	Gut	18:20	arg1	Microbiota					22:31	Human Gut Microbiota	12:31	Human Gut Microbiota	12:31	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	6	36	theme	proficient	1577:1586	arg1	Bifidobacteria					1554:1567	Bifidobacteria	1554:1567	Bifidobacteria	1554:1567	Bifidobacteria, though proficient at degrading RS and inulin, may not contribute to the butyrogenic effect of those fermentable fibers in the short term.					
30696735	4	37	theme	inulin	1037:1042	arg1	changes					1062:1068	inulin induced different changes	1037:1068	inulin induced different changes	1037:1068	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	38	theme	specific	1412:1419	arg1	supplement					1429:1438	a specific dietary supplement	1410:1438	a specific dietary supplement	1410:1438	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	0	39	theme	Human	12:16	arg1	Microbiota					22:31	Human Gut Microbiota	12:31	Human Gut Microbiota	12:31	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	0	40	theme	Fermentable	109:119	arg1	Fibers					121:126	Three Fermentable Fibers	103:126	Three Fermentable Fibers	103:126	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	4	41	from	RMS	1029:1031	arg1	communities					1079:1089	fecal communities	1073:1089	fecal communities	1073:1089	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	2	42	theme	resistant	477:485	arg1	starch					487:492	resistant starch	477:492	resistant starch from maize (RMS)	477:509	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	5	43	from	production	1524:1533	arg1	response					1538:1545	response	1538:1545	response to RS	1538:1551	In particular, R. bromii or C. chartatabidum may be required for enhanced butyrate production in response to RS.					
30696735	4	44	theme	different	1052:1060	arg1	changes					1062:1068	inulin induced different changes	1037:1068	inulin induced different changes	1037:1068	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	0	45	theme	Microbiota	22:31	arg1	Dynamics					0:7	Dynamics	0:7	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.	0:127	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	2	46	from	maize	499:503	arg1	starch					487:492	resistant starch	477:492	resistant starch from maize (RMS)	477:509	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	46	from	maize	499:503	arg1	control					567:573	an accessible corn starch control	541:573	an accessible corn starch control	541:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	46	from	maize	499:503	arg1	RPS					471:473	RPS	471:473	RPS	471:473	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	46	from	maize	499:503	arg1	inulin					512:517	inulin	512:517	inulin from chicory root	512:535	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	46	from	maize	499:503	arg1	potatoes					461:468	potatoes	461:468	potatoes (RPS)	461:474	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	4	47	from	increases	1130:1138	arg1	levels.IMPORTANCE					1158:1174	fecal butyrate levels.IMPORTANCE	1143:1174	fecal butyrate levels.IMPORTANCE	1143:1174	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	48	theme	significant	1118:1128	arg1	increases					1130:1138	significant increases	1118:1138	significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement	1118:1438	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	49	theme	individual	1340:1349	arg1	microbiota					1353:1362	an individual's microbiota	1337:1362	an individual's microbiota	1337:1362	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	0	50	theme	Fatty	49:53	arg1	Acids					55:59	Short-Chain Fatty Acids	37:59	Short-Chain Fatty Acids	37:59	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	2	51	from	root	532:535	arg1	starch					487:492	resistant starch	477:492	resistant starch from maize (RMS)	477:509	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	51	from	root	532:535	arg1	control					567:573	an accessible corn starch control	541:573	an accessible corn starch control	541:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	51	from	root	532:535	arg1	RPS					471:473	RPS	471:473	RPS	471:473	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	51	from	root	532:535	arg1	inulin					512:517	inulin	512:517	inulin from chicory root	512:535	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	51	from	root	532:535	arg1	potatoes					461:468	potatoes	461:468	potatoes (RPS)	461:474	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	4	52	theme	higher	880:885	arg1	concentrations					896:909	higher butyrate concentrations	880:909	higher butyrate concentrations	880:909	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	3	53	from	increase	605:612	arg1	SCFAs					623:627	total SCFAs	617:627	total SCFAs	617:627	RPS resulted in the greatest increase in total SCFAs, including butyrate.					
30696735	3	53	from	increase	605:612	arg1	butyrate					640:647	butyrate	640:647	butyrate	640:647	RPS resulted in the greatest increase in total SCFAs, including butyrate.					
30696735	0	54	theme	Short-Chain	37:47	arg1	Acids					55:59	Short-Chain Fatty Acids	37:59	Short-Chain Fatty Acids	37:59	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	6	55	theme	fermentable	1670:1680	arg1	fibers					1682:1687	those fermentable fibers	1664:1687	those fermentable fibers	1664:1687	Bifidobacteria, though proficient at degrading RS and inulin, may not contribute to the butyrogenic effect of those fermentable fibers in the short term.					
30696735	4	56	theme	fermentable	1210:1220	arg1	fibers					1222:1227	not all fermentable fibers	1202:1227	not all fermentable fibers	1202:1227	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	57	theme	relative	726:733	arg1	abundance					735:743	the relative abundance	722:743	the relative abundance of bifidobacteria	722:761	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	58	theme	fecal	1143:1147	arg1	levels.IMPORTANCE					1158:1174	fecal butyrate levels.IMPORTANCE	1143:1174	fecal butyrate levels.IMPORTANCE	1143:1174	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	59	theme	rectale	1021:1027	arg1	RMS					1029:1031	rectale RMS	1021:1031	rectale RMS	1021:1031	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	60	theme	composition	1322:1332	arg1	importance					1304:1313	the importance	1300:1313	the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement	1300:1438	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	2	61	from	potatoes	461:468	arg1	starch					449:454	resistant starch	439:454	resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control	439:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	2	62	from	control	567:573	arg1	starch					449:454	resistant starch	439:454	resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control	439:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
30696735	4	63	with	replete	950:956	arg1	populations					963:973	populations	963:973	populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities	963:1089	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	5	64	theme	enhanced	1506:1513	arg1	production					1524:1533	enhanced butyrate production	1506:1533	enhanced butyrate production in response to RS	1506:1551	In particular, R. bromii or C. chartatabidum may be required for enhanced butyrate production in response to RS.					
30696735	4	65	from	increases	709:717	arg1	abundance					735:743	the relative abundance	722:743	the relative abundance of bifidobacteria	722:761	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	66	dep	increases	1130:1138	arg1	reveal					1190:1195	reveal	1190:1195	reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement	1190:1438	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	1	67	theme	gut	204:206	arg1	microbiome					208:217	the gut microbiome	200:217	the gut microbiome	200:217	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	4	68	theme	butyrate	887:894	arg1	concentrations					896:909	higher butyrate concentrations	880:909	higher butyrate concentrations	880:909	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	69	theme	dietary	1421:1427	arg1	supplement					1429:1438	a specific dietary supplement	1410:1438	a specific dietary supplement	1410:1438	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	4	70	from	changes	1062:1068	arg1	communities					1079:1089	fecal communities	1073:1089	fecal communities	1073:1089	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	5	71	dep	R.	1456:1457	arg1	bromii					1459:1464	R. bromii	1456:1464	R. bromii	1456:1464	In particular, R. bromii or C. chartatabidum may be required for enhanced butyrate production in response to RS.					
30696735	4	72	dep	RMS	1029:1031	arg1	Eubacterium					1009:1019	Eubacterium	1009:1019	Eubacterium	1009:1019	Although the majority of microbiomes responded to RPS with increases in the relative abundance of bifidobacteria, those that responded with an increase in Ruminococcus bromii or Clostridium chartatabidum were more likely to yield higher butyrate concentrations, especially when their microbiota were replete with populations of the butyrate-producing species Eubacterium rectale RMS and inulin induced different changes in fecal communities, but they did not generate significant increases in fecal butyrate levels.IMPORTANCE These results reveal that not all fermentable fibers are equally capable of stimulating SCFA production, and they highlight the importance of the composition of an individual's microbiota in determining whether or not they respond to a specific dietary supplement.					
30696735	0	73	theme	Acids	55:59	arg1	Dynamics					0:7	Dynamics	0:7	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.	0:127	Dynamics of Human Gut Microbiota and Short-Chain Fatty Acids in Response to Dietary Interventions with Three Fermentable Fibers.					
30696735	1	74	theme	fermentable	291:301	arg1	fiber					303:307	fermentable fiber	291:307	fermentable fiber	291:307	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	1	75	theme	short-chain	143:153	arg1	butyrate					187:194	butyrate	187:194	butyrate	187:194	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	1	75	theme	short-chain	143:153	arg1	SCFAs					168:172	SCFAs	168:172	SCFAs	168:172	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	1	75	theme	short-chain	143:153	arg1	acids					161:165	short-chain fatty acids	143:165	short-chain fatty acids (SCFAs)	143:173	Production of short-chain fatty acids (SCFAs), especially butyrate, in the gut microbiome is required for optimal health but is frequently limited by the lack of fermentable fiber in the diet.					
30696735	2	76	from	starch	487:492	arg1	starch					449:454	resistant starch	439:454	resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control	439:573	We attempted to increase butyrate production by supplementing the diets of 174 healthy young adults for 2 weeks with resistant starch from potatoes (RPS), resistant starch from maize (RMS), inulin from chicory root, or an accessible corn starch control.					
31476399	0	0	theme	structural	67:76	arg1	properties					96:105	the physical, mechanical, structural and antibacterial properties	41:105	the physical, mechanical, structural and antibacterial properties of chitosan film for quail	41:132	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	8	1	contain	had	1396:1398	arg2	effect					1420:1425	a good preservation effect	1400:1425	a good preservation effect	1400:1425	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	8	1	contain	had	1396:1398	arg1	film					1391:1394	the coating film	1379:1394	the coating film	1379:1394	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	8	1	contain	had	1396:1398	arg1	%					1365:1365	0.25%	1361:1365	0.25%	1361:1365	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	8	1	contain	had	1396:1398	arg1	%					1374:1374	0.5%	1371:1374	0.5%	1371:1374	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	7	2	theme	chitosan-SA	1139:1149	arg1	films					1151:1155	chitosan-SA films	1139:1155	chitosan-SA films incorporating 0.25% and 0.5% of SA	1139:1190	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	7	2	theme	chitosan-SA	1139:1149	arg1	materials					1234:1242	active packing materials	1219:1242	active packing materials for food preservation	1219:1264	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	1	3	theme	syringic	243:250	arg1	acid					252:255	syringic acid	243:255	syringic acid (SA)	243:260	The physical mechanics and structural properties of composite films based on chitosan and syringic acid (SA) were studied in this study.					
31476399	1	3	theme	syringic	243:250	arg1	SA					258:259	SA	258:259	SA	258:259	The physical mechanics and structural properties of composite films based on chitosan and syringic acid (SA) were studied in this study.					
31476399	5	4	theme	XRD	711:713	arg1	spectra					715:721	The FT-IR and XRD spectra	697:721	spectra	715:721	The FT-IR and XRD spectra of the chitosan-SA film indicated that the interaction between chitosan and SA may be non-covalent, and DSC indicated that the thermal stability of the composite film was reduced.					
31476399	8	5	used	used	1290:1293	arg2	film					1281:1284	The composite film	1267:1284	The composite film	1267:1284	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	6	6	from	color	928:932	arg1	properties					1035:1044	mechanical properties	1024:1044	mechanical properties	1024:1044	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	6	7	theme	slight	1006:1011	arg1	changes					1013:1019	the slight changes	1002:1019	the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties	915:1044	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	0	8	theme	antibacterial	82:94	arg1	properties					96:105	the physical, mechanical, structural and antibacterial properties	41:105	the physical, mechanical, structural and antibacterial properties of chitosan film for quail	41:132	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	0	9	from	Effect	0:5	arg1	properties					96:105	the physical, mechanical, structural and antibacterial properties	41:105	the physical, mechanical, structural and antibacterial properties of chitosan film for quail	41:132	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	3	10	theme	Chitosan-SA	499:509	arg1	films					511:515	Chitosan-SA films	499:515	Chitosan-SA films	499:515	In addition, Chitosan-SA films had a significant antibacterial effect on test bacteria.					
31476399	6	11	theme	water-blocking	964:977	arg1	properties					979:988	increased bacteriostatic and water-blocking properties	935:988	increased bacteriostatic and water-blocking properties	935:988	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	6	11	theme	water-blocking	964:977	arg1	color					928:932	the modified color	915:932	the modified color	915:932	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	4	12	theme	0f	690:691	arg1	SA					693:694	0.25% and 0.5% 0f SA	675:694	0.25% and 0.5% 0f SA	675:694	The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA.					
31476399	4	13	theme	%	688:688	arg1	SA					693:694	0.25% and 0.5% 0f SA	675:694	0.25% and 0.5% 0f SA	675:694	The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA.					
31476399	1	14	theme	physical	157:164	arg1	mechanics					166:174	physical mechanics	157:174	physical mechanics	157:174	The physical mechanics and structural properties of composite films based on chitosan and syringic acid (SA) were studied in this study.					
31476399	8	15	theme	coating	1383:1389	arg1	film					1391:1394	the coating film	1379:1394	the coating film	1379:1394	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	6	16	theme	modified	919:926	arg1	properties					979:988	increased bacteriostatic and water-blocking properties	935:988	increased bacteriostatic and water-blocking properties	935:988	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	6	16	theme	modified	919:926	arg1	color					928:932	the modified color	915:932	the modified color	915:932	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	6	17	theme	increased	935:943	arg1	properties					979:988	increased bacteriostatic and water-blocking properties	935:988	increased bacteriostatic and water-blocking properties	935:988	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	6	17	theme	increased	935:943	arg1	color					928:932	the modified color	915:932	the modified color	915:932	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	7	18	theme	active	1219:1224	arg1	materials					1234:1242	active packing materials	1219:1242	active packing materials for food preservation	1219:1264	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	7	18	theme	active	1219:1224	arg1	films					1151:1155	chitosan-SA films	1139:1155	chitosan-SA films incorporating 0.25% and 0.5% of SA	1139:1190	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	5	19	theme	film	742:745	arg1	spectra					715:721	The FT-IR and XRD spectra	697:721	spectra	715:721	The FT-IR and XRD spectra of the chitosan-SA film indicated that the interaction between chitosan and SA may be non-covalent, and DSC indicated that the thermal stability of the composite film was reduced.					
31476399	4	20	theme	chitosan-SA	607:617	arg1	films					619:623	chitosan-SA films	607:623	chitosan-SA films	607:623	The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA.					
31476399	3	21	theme	significant	523:533	arg1	effect					549:554	a significant antibacterial effect	521:554	a significant antibacterial effect	521:554	In addition, Chitosan-SA films had a significant antibacterial effect on test bacteria.					
31476399	8	22	theme	quail	1319:1323	arg1	coatings					1329:1336	quail egg coatings	1319:1336	quail egg coatings	1319:1336	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	2	23	theme	chitosan-SA	345:355	arg1	films					357:361	the chitosan-SA films	341:361	the chitosan-SA films exhibited higher density	341:386	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
31476399	2	23	theme	chitosan-SA	345:355	arg1	opacity					410:416	opacity	410:416	opacity	410:416	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
31476399	2	23	theme	chitosan-SA	345:355	arg1	solubility					395:404	water solubility	389:404	water solubility	389:404	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
31476399	3	24	theme	antibacterial	535:547	arg1	effect					549:554	a significant antibacterial effect	521:554	a significant antibacterial effect	521:554	In addition, Chitosan-SA films had a significant antibacterial effect on test bacteria.					
31476399	1	25	theme	structural	180:189	arg1	properties					191:200	structural properties	180:200	structural properties	180:200	The physical mechanics and structural properties of composite films based on chitosan and syringic acid (SA) were studied in this study.					
31476399	0	26	theme	acid	19:22	arg1	incorporation					24:36	syringic acid incorporation	10:36	syringic acid incorporation	10:36	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	3	27	contain	had	517:519	arg2	effect					549:554	a significant antibacterial effect	521:554	a significant antibacterial effect	521:554	In addition, Chitosan-SA films had a significant antibacterial effect on test bacteria.					
31476399	3	27	contain	had	517:519	arg1	films					511:515	Chitosan-SA films	499:515	Chitosan-SA films	499:515	In addition, Chitosan-SA films had a significant antibacterial effect on test bacteria.					
31476399	0	28	theme	film	119:122	arg1	properties					96:105	the physical, mechanical, structural and antibacterial properties	41:105	the physical, mechanical, structural and antibacterial properties of chitosan film for quail	41:132	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	6	29	theme	food	1127:1130	arg1	life					1115:1118	the shelf life	1105:1118	the shelf life of the food	1105:1130	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	5	30	theme	composite	875:883	arg1	film					885:888	the composite film	871:888	the composite film	871:888	The FT-IR and XRD spectra of the chitosan-SA film indicated that the interaction between chitosan and SA may be non-covalent, and DSC indicated that the thermal stability of the composite film was reduced.					
31476399	2	31	theme	water	389:393	arg1	films					357:361	the chitosan-SA films	341:361	the chitosan-SA films exhibited higher density	341:386	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
31476399	2	31	theme	water	389:393	arg1	solubility					395:404	water solubility	389:404	water solubility	389:404	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
31476399	0	32	theme	syringic	10:17	arg1	incorporation					24:36	syringic acid incorporation	10:36	syringic acid incorporation	10:36	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	8	33	theme	good	1402:1405	arg1	effect					1420:1425	a good preservation effect	1400:1425	a good preservation effect	1400:1425	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	0	34	theme	chitosan	110:117	arg1	film					119:122	chitosan film	110:122	chitosan film for quail	110:132	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	8	35	theme	coatings	1329:1336	arg1	preservation					1303:1314	the preservation	1299:1314	the preservation of quail egg coatings	1299:1336	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	5	36	theme	film	885:888	arg1	stability					858:866	the thermal stability	846:866	the thermal stability of the composite film	846:888	The FT-IR and XRD spectra of the chitosan-SA film indicated that the interaction between chitosan and SA may be non-covalent, and DSC indicated that the thermal stability of the composite film was reduced.					
31476399	6	37	theme	SA	1077:1078	arg1	addition					1065:1072	the addition	1061:1072	the addition of SA	1061:1078	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	2	38	theme	vapor	433:437	arg1	permeability					439:450	the water vapor permeability	423:450	the water vapor permeability	423:450	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
31476399	7	39	theme	SA	1189:1190	arg1	SA					1189:1190	SA	1189:1190	SA	1189:1190	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	7	39	theme	SA	1189:1190	arg1	%					1175:1175	0.25%	1171:1175	0.25%	1171:1175	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	7	39	theme	SA	1189:1190	arg1	%					1184:1184	0.5%	1181:1184	0.5% of SA	1181:1190	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	4	40	theme	films	619:623	arg1	uniform					635:641	uniform	635:641	uniform	635:641	The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA.					
31476399	4	40	theme	films	619:623	arg1	cross-section					590:602	The surface and cross-section	574:602	cross-section	590:602	The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA.					
31476399	4	40	theme	films	619:623	arg1	surface					578:584	The surface and cross-section	574:602	surface	578:584	The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA.					
31476399	3	41	theme	test	559:562	arg1	bacteria					564:571	test bacteria	559:571	test bacteria	559:571	In addition, Chitosan-SA films had a significant antibacterial effect on test bacteria.					
31476399	1	42	theme	composite	205:213	arg1	films					215:219	composite films	205:219	composite films based on chitosan and syringic acid (SA)	205:260	The physical mechanics and structural properties of composite films based on chitosan and syringic acid (SA) were studied in this study.					
31476399	2	43	theme	water	427:431	arg1	permeability					439:450	the water vapor permeability	423:450	the water vapor permeability	423:450	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
31476399	0	44	theme	incorporation	24:36	arg1	Effect					0:5	Effect	0:5	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail	0:132	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	1	45	theme	films	215:219	arg1	mechanics					166:174	physical mechanics	157:174	physical mechanics	157:174	The physical mechanics and structural properties of composite films based on chitosan and syringic acid (SA) were studied in this study.					
31476399	1	45	theme	films	215:219	arg1	properties					191:200	structural properties	180:200	structural properties	180:200	The physical mechanics and structural properties of composite films based on chitosan and syringic acid (SA) were studied in this study.					
31476399	0	46	theme	physical	45:52	arg1	properties					96:105	the physical, mechanical, structural and antibacterial properties	41:105	the physical, mechanical, structural and antibacterial properties of chitosan film for quail	41:132	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	8	47	theme	preservation	1407:1418	arg1	effect					1420:1425	a good preservation effect	1400:1425	a good preservation effect	1400:1425	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	5	48	theme	FT-IR	701:705	arg1	spectra					715:721	The FT-IR and XRD spectra	697:721	spectra	715:721	The FT-IR and XRD spectra of the chitosan-SA film indicated that the interaction between chitosan and SA may be non-covalent, and DSC indicated that the thermal stability of the composite film was reduced.					
31476399	4	49	theme	%	679:679	arg1	SA					693:694	0.25% and 0.5% 0f SA	675:694	0.25% and 0.5% 0f SA	675:694	The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA.					
31476399	5	50	theme	chitosan-SA	730:740	arg1	film					742:745	the chitosan-SA film	726:745	the chitosan-SA film	726:745	The FT-IR and XRD spectra of the chitosan-SA film indicated that the interaction between chitosan and SA may be non-covalent, and DSC indicated that the thermal stability of the composite film was reduced.					
31476399	1	51	dep	mechanics	166:174	arg1	The					153:155	The	153:155	The	153:155	The physical mechanics and structural properties of composite films based on chitosan and syringic acid (SA) were studied in this study.					
31476399	4	52	theme	0.25	675:678	arg1	%					679:679	%	679:679	%	679:679	The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA.					
31476399	8	53	theme	egg	1325:1327	arg1	coatings					1329:1336	quail egg coatings	1319:1336	quail egg coatings	1319:1336	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	4	54	theme	0.5	685:687	arg1	%					688:688	%	688:688	%	688:688	The surface and cross-section of chitosan-SA films were more uniform and smoother when combined with 0.25% and 0.5% 0f SA.					
31476399	8	55	theme	composite	1271:1279	arg1	film					1281:1284	The composite film	1267:1284	The composite film	1267:1284	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	6	56	theme	bacteriostatic	945:958	arg1	properties					979:988	increased bacteriostatic and water-blocking properties	935:988	increased bacteriostatic and water-blocking properties	935:988	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	6	56	theme	bacteriostatic	945:958	arg1	color					928:932	the modified color	915:932	the modified color	915:932	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	7	57	theme	food	1248:1251	arg1	preservation					1253:1264	food preservation	1248:1264	food preservation	1248:1264	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	6	58	from	changes	1013:1019	arg1	properties					1035:1044	mechanical properties	1024:1044	mechanical properties	1024:1044	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	5	59	theme	thermal	850:856	arg1	stability					858:866	the thermal stability	846:866	the thermal stability of the composite film	846:888	The FT-IR and XRD spectra of the chitosan-SA film indicated that the interaction between chitosan and SA may be non-covalent, and DSC indicated that the thermal stability of the composite film was reduced.					
31476399	0	60	theme	mechanical	55:64	arg1	properties					96:105	the physical, mechanical, structural and antibacterial properties	41:105	the physical, mechanical, structural and antibacterial properties of chitosan film for quail	41:132	Effect of syringic acid incorporation on the physical, mechanical, structural and antibacterial properties of chitosan film for quail eggs preservation.					
31476399	2	61	theme	higher	373:378	arg1	density					380:386	higher density	373:386	higher density	373:386	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
31476399	7	62	theme	packing	1226:1232	arg1	materials					1234:1242	active packing materials	1219:1242	active packing materials for food preservation	1219:1264	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	7	62	theme	packing	1226:1232	arg1	films					1151:1155	chitosan-SA films	1139:1155	chitosan-SA films incorporating 0.25% and 0.5% of SA	1139:1190	Thus, chitosan-SA films incorporating 0.25% and 0.5% of SA can be further explored as active packing materials for food preservation.					
31476399	8	63	theme	film	1391:1394	arg1	film					1391:1394	the coating film	1379:1394	the coating film	1379:1394	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	8	63	theme	film	1391:1394	arg1	%					1365:1365	0.25%	1361:1365	0.25%	1361:1365	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	8	63	theme	film	1391:1394	arg1	%					1374:1374	0.5%	1371:1374	0.5%	1371:1374	The composite film was used for the preservation of quail egg coatings, and it was found that 0.25% and 0.5% of the coating film had a good preservation effect.					
31476399	6	64	theme	mechanical	1024:1033	arg1	properties					1035:1044	mechanical properties	1024:1044	mechanical properties	1024:1044	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	2	65	theme	water	456:460	arg1	content					462:468	water content	456:468	water content	456:468	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
31476399	6	66	theme	shelf	1109:1113	arg1	life					1115:1118	the shelf life	1105:1118	the shelf life of the food	1105:1130	In summary, the modified color, increased bacteriostatic and water-blocking properties, as well as the slight changes in mechanical properties indicated that the addition of SA may contribute to extend the shelf life of the food.					
31476399	2	67	theme	SA	333:334	arg1	addition					321:328	the addition	317:328	the addition of SA	317:334	The results indicated that the addition of SA made the chitosan-SA films exhibited higher density, water solubility and opacity, but the water vapor permeability and water content were decreased.					
29480418	3	0	dep	%	327:327	arg1	w/v					330:332	w/v	330:332	w/v	330:332	Strain CAU 1455T grew optimally at 30 °C and at pH 7.5 in the presence of 1% (w/v) NaCl.					
29480418	8	1	theme	phenotypic	847:856	arg1	data					887:890	phenotypic, chemotaxonomic, and genetic data	847:890	phenotypic, chemotaxonomic, and genetic data	847:890	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	4	2	dep	Cellulosimicrobium	504:521	arg1	terreum					523:529	terreum	523:529	terreum	523:529	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	2	3	attach	isolated	216:223	arg1	sand					246:249	marine sediment sand	230:249	marine sediment sand	230:249	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	2	3	attach	isolated	216:223	arg2	strain					182:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain	74:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain	74:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	4	4	theme	genus	447:451	arg1	Cellulosimicrobium					453:470	the genus Cellulosimicrobium	443:470	the genus Cellulosimicrobium	443:470	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	7	5	theme	relatedness	775:785	arg1	%					835:835	27.8%	831:835	27.8%	831:835	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	7	5	theme	relatedness	775:785	arg1	level					758:762	the level	754:762	the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T	754:825	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	5	6	contain	possessed	569:577	arg2	H4					585:586	H4	585:586	H4	585:586	The strain possessed MK-9 (H4) as the predominant menaquinone and anteiso-C15:0 as the major cellular fatty acids.					
29480418	5	6	contain	possessed	569:577	arg2	MK-9					579:582	MK-9	579:582	MK-9 (H4)	579:587	The strain possessed MK-9 (H4) as the predominant menaquinone and anteiso-C15:0 as the major cellular fatty acids.					
29480418	5	6	contain	possessed	569:577	arg1	strain					562:567	The strain	558:567	The strain	558:567	The strain possessed MK-9 (H4) as the predominant menaquinone and anteiso-C15:0 as the major cellular fatty acids.					
29480418	5	7	theme	cellular	651:658	arg1	acids					666:670	the major cellular fatty acids	641:670	the major cellular fatty acids	641:670	The strain possessed MK-9 (H4) as the predominant menaquinone and anteiso-C15:0 as the major cellular fatty acids.					
29480418	8	8	theme	novel	923:927	arg1	species					929:935	a novel species	921:935	a novel species	921:935	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	7	9	dep	C.	809:810	arg1	terreum					812:818	terreum	812:818	terreum	812:818	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	1	10	theme	Marine	52:57	arg1	Sand					68:71	Marine Sediment Sand	52:71	Marine Sediment Sand	52:71	nov., Isolated from Marine Sediment Sand.					
29480418	10	11	theme	= KCTC	1069:1074	arg1	1455T					1062:1066	CAU 1455T	1058:1066	CAU 1455T (= KCTC 49039T = NBRC 113062T)	1058:1097	The type strain is CAU 1455T (= KCTC 49039T = NBRC 113062T).					
29480418	10	11	theme	= KCTC	1069:1074	arg1	113062T					1090:1096	= KCTC 49039T = NBRC 113062T	1069:1096	= KCTC 49039T = NBRC 113062T	1069:1096	The type strain is CAU 1455T (= KCTC 49039T = NBRC 113062T).					
29480418	3	12	theme	1	326:326	arg1	%					327:327	%	327:327	%	327:327	Strain CAU 1455T grew optimally at 30 °C and at pH 7.5 in the presence of 1% (w/v) NaCl.					
29480418	4	13	theme	CAU	415:417	arg1	1455T					419:423	strain CAU 1455T	408:423	strain CAU 1455T	408:423	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	8	14	theme	genetic	879:885	arg1	data					887:890	phenotypic, chemotaxonomic, and genetic data	847:890	phenotypic, chemotaxonomic, and genetic data	847:890	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	1	15	theme	Sediment	59:66	arg1	Sand					68:71	Marine Sediment Sand	52:71	Marine Sediment Sand	52:71	nov., Isolated from Marine Sediment Sand.					
29480418	4	16	theme	strain	408:413	arg1	1455T					419:423	strain CAU 1455T	408:423	strain CAU 1455T	408:423	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	4	17	theme	Phylogenetic	341:352	arg1	analysis					354:361	Phylogenetic analysis	341:361	Phylogenetic analysis based on 16S rRNA gene sequences	341:394	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	7	18	theme	DNA-DNA	767:773	arg1	relatedness					775:785	DNA-DNA relatedness	767:785	DNA-DNA relatedness	767:785	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	10	19	theme	CAU	1058:1060	arg1	strain					1048:1053	The type strain	1039:1053	The type strain	1039:1053	The type strain is CAU 1455T (= KCTC 49039T = NBRC 113062T).					
29480418	10	19	theme	CAU	1058:1060	arg1	1455T					1062:1066	CAU 1455T	1058:1066	CAU 1455T (= KCTC 49039T = NBRC 113062T)	1058:1097	The type strain is CAU 1455T (= KCTC 49039T = NBRC 113062T).					
29480418	10	19	theme	CAU	1058:1060	arg1	113062T					1090:1096	= KCTC 49039T = NBRC 113062T	1069:1096	= KCTC 49039T = NBRC 113062T	1069:1096	The type strain is CAU 1455T (= KCTC 49039T = NBRC 113062T).					
29480418	8	20	dep	Cellulosimicrobium	989:1006	arg1	arenosum					1008:1015	arenosum	1008:1015	arenosum	1008:1015	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	0	21	theme	arenosum	19:26	arg1	sp					28:29	Cellulosimicrobium arenosum sp	0:29	Cellulosimicrobium arenosum sp.	0:30	Cellulosimicrobium arenosum sp.					
29480418	8	22	theme	genus	944:948	arg1	Cellulosimicrobium					950:967	the genus Cellulosimicrobium	940:967	the genus Cellulosimicrobium	940:967	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	0	23	theme	Cellulosimicrobium	0:17	arg1	sp					28:29	Cellulosimicrobium arenosum sp	0:29	Cellulosimicrobium arenosum sp.	0:30	Cellulosimicrobium arenosum sp.					
29480418	7	24	theme	G+C	724:726	arg1	%					748:748	74.3 mol%	740:748	74.3 mol%	740:748	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	7	24	theme	G+C	724:726	arg1	content					728:734	The DNA G+C content	716:734	The DNA G+C content	716:734	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	8	25	theme	CAU	900:902	arg1	1455T					904:908	strain CAU 1455T	893:908	strain CAU 1455T	893:908	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	4	26	theme	gene	381:384	arg1	sequences					386:394	16S rRNA gene sequences	372:394	16S rRNA gene sequences	372:394	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	3	27	theme	%	327:327	arg1	NaCl					335:338	1% (w/v) NaCl	326:338	1% (w/v) NaCl	326:338	Strain CAU 1455T grew optimally at 30 °C and at pH 7.5 in the presence of 1% (w/v) NaCl.					
29480418	8	28	theme	strain	893:898	arg1	1455T					904:908	strain CAU 1455T	893:908	strain CAU 1455T	893:908	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	10	29	theme	49039T = NBRC	1076:1088	arg1	1455T					1062:1066	CAU 1455T	1058:1066	CAU 1455T (= KCTC 49039T = NBRC 113062T)	1058:1097	The type strain is CAU 1455T (= KCTC 49039T = NBRC 113062T).					
29480418	10	29	theme	49039T = NBRC	1076:1088	arg1	113062T					1090:1096	= KCTC 49039T = NBRC 113062T	1069:1096	= KCTC 49039T = NBRC 113062T	1069:1096	The type strain is CAU 1455T (= KCTC 49039T = NBRC 113062T).					
29480418	4	30	theme	Cellulosimicrobium	504:521	arg1	DS-61T					531:536	Cellulosimicrobium terreum DS-61T	504:536	Cellulosimicrobium terreum DS-61T (similarity 97.9%)	504:555	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	8	31	theme	Cellulosimicrobium	950:967	arg1	species					929:935	a novel species	921:935	a novel species	921:935	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	7	32	theme	DNA	720:722	arg1	%					748:748	74.3 mol%	740:748	74.3 mol%	740:748	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	7	32	theme	DNA	720:722	arg1	content					728:734	The DNA G+C content	716:734	The DNA G+C content	716:734	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	3	33	theme	Strain	252:257	arg1	1455T					263:267	Strain CAU 1455T	252:267	Strain CAU 1455T	252:267	Strain CAU 1455T grew optimally at 30 °C and at pH 7.5 in the presence of 1% (w/v) NaCl.					
29480418	2	34	theme	bacterial	172:180	arg1	strain					182:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain	74:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain	74:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	7	35	theme	74.3 mol	740:747	arg1	%					748:748	74.3 mol%	740:748	74.3 mol%	740:748	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	7	35	theme	74.3 mol	740:747	arg1	content					728:734	The DNA G+C content	716:734	The DNA G+C content	716:734	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	10	36	theme	type	1043:1046	arg1	strain					1048:1053	The type strain	1039:1053	The type strain	1039:1053	The type strain is CAU 1455T (= KCTC 49039T = NBRC 113062T).					
29480418	10	36	theme	type	1043:1046	arg1	1455T					1062:1066	CAU 1455T	1058:1066	CAU 1455T (= KCTC 49039T = NBRC 113062T)	1058:1097	The type strain is CAU 1455T (= KCTC 49039T = NBRC 113062T).					
29480418	3	37	theme	CAU	259:261	arg1	1455T					263:267	Strain CAU 1455T	252:267	Strain CAU 1455T	252:267	Strain CAU 1455T grew optimally at 30 °C and at pH 7.5 in the presence of 1% (w/v) NaCl.					
29480418	2	38	theme	rod-shaped	161:170	arg1	strain					182:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain	74:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain	74:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	3	39	theme	NaCl	335:338	arg1	presence					314:321	the presence	310:321	the presence of 1% (w/v) NaCl	310:338	Strain CAU 1455T grew optimally at 30 °C and at pH 7.5 in the presence of 1% (w/v) NaCl.					
29480418	5	40	dep	menaquinone	608:618	arg1	the					592:594	the	592:594	the	592:594	The strain possessed MK-9 (H4) as the predominant menaquinone and anteiso-C15:0 as the major cellular fatty acids.					
29480418	8	41	theme	name	984:987	arg1	sp					1017:1018	the name Cellulosimicrobium arenosum sp	980:1018	the name Cellulosimicrobium arenosum sp	980:1018	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	5	42	theme	major	645:649	arg1	acids					666:670	the major cellular fatty acids	641:670	the major cellular fatty acids	641:670	The strain possessed MK-9 (H4) as the predominant menaquinone and anteiso-C15:0 as the major cellular fatty acids.					
29480418	2	43	theme	Gram-stain-positive	76:94	arg1	strain					182:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain	74:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain	74:187	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	5	44	theme	predominant	596:606	arg1	menaquinone					608:618	menaquinone	608:618	menaquinone	608:618	The strain possessed MK-9 (H4) as the predominant menaquinone and anteiso-C15:0 as the major cellular fatty acids.					
29480418	4	45	dep	DS-61T	531:536	arg1	similarity					539:548	similarity	539:548	similarity 97.9%	539:554	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	2	46	theme	CAU	201:203	arg1	1455T					205:209	CAU 1455T	201:209	CAU 1455T	201:209	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	6	47	theme	Peptidoglycan	673:685	arg1	type					687:690	Peptidoglycan type	673:690	Peptidoglycan type	673:690	Peptidoglycan type was A4a (L-Lys-D-Glu2).					
29480418	4	48	theme	%	554:554	arg1	similarity					539:548	similarity	539:548	similarity 97.9%	539:554	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	8	49	theme	Cellulosimicrobium	989:1006	arg1	sp					1017:1018	the name Cellulosimicrobium arenosum sp	980:1018	the name Cellulosimicrobium arenosum sp	980:1018	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	4	50	theme	rRNA	376:379	arg1	sequences					386:394	16S rRNA gene sequences	372:394	16S rRNA gene sequences	372:394	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	4	51	theme	97.9	550:553	arg1	%					554:554	%	554:554	%	554:554	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	4	52	theme	16S	372:374	arg1	sequences					386:394	16S rRNA gene sequences	372:394	16S rRNA gene sequences	372:394	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1455T was affiliated to the genus Cellulosimicrobium and was most closely related to Cellulosimicrobium terreum DS-61T (similarity 97.9%).					
29480418	2	53	theme	sediment	237:244	arg1	sand					246:249	marine sediment sand	230:249	marine sediment sand	230:249	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	8	54	theme	chemotaxonomic	859:872	arg1	data					887:890	phenotypic, chemotaxonomic, and genetic data	847:890	phenotypic, chemotaxonomic, and genetic data	847:890	Based on phenotypic, chemotaxonomic, and genetic data, strain CAU 1455T represents a novel species of the genus Cellulosimicrobium, for which the name Cellulosimicrobium arenosum sp.					
29480418	7	55	theme	C.	809:810	arg1	DS-61T					820:825	C. terreum DS-61T	809:825	C. terreum DS-61T	809:825	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	2	56	dep	Gram-stain-positive	76:94	arg1	pigmented					123:131	pigmented	123:131	pigmented	123:131	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	2	56	dep	Gram-stain-positive	76:94	arg1	short					155:159	short	155:159	short	155:159	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	2	56	dep	Gram-stain-positive	76:94	arg1	aerobic					146:152	aerobic	146:152	aerobic	146:152	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	2	56	dep	Gram-stain-positive	76:94	arg1	non-motile					134:143	non-motile	134:143	non-motile	134:143	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	2	56	dep	Gram-stain-positive	76:94	arg1	non-spore-forming					97:113	non-spore-forming	97:113	non-spore-forming	97:113	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	2	57	theme	marine	230:235	arg1	sand					246:249	marine sediment sand	230:249	marine sediment sand	230:249	A Gram-stain-positive, non-spore-forming, yellow pigmented, non-motile, aerobic, short rod-shaped bacterial strain, designated CAU 1455T, was isolated from marine sediment sand.					
29480418	7	58	theme	CAU	795:797	arg1	1455T					799:803	CAU 1455T	795:803	CAU 1455T	795:803	The DNA G+C content was 74.3 mol% and the level of DNA-DNA relatedness between CAU 1455T and C. terreum DS-61T was 27.8%.					
29480418	5	59	theme	fatty	660:664	arg1	acids					666:670	the major cellular fatty acids	641:670	the major cellular fatty acids	641:670	The strain possessed MK-9 (H4) as the predominant menaquinone and anteiso-C15:0 as the major cellular fatty acids.					
29535329	1	0	with	association	151:161	arg1	lipids					207:212	lipids	207:212	lipids	207:212	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	0	with	association	151:161	arg1	drugs					219:223	drugs	219:223	drugs	219:223	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	0	with	association	151:161	arg1	proteins					197:204	other proteins	191:204	other proteins	191:204	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	4	1	used	used	561:564	arg2	approaches					543:552	Statistical approaches	531:552	Statistical approaches	531:552	Statistical approaches can be used to derive such potentials; however, this requires a sufficient number of MP structures.					
29535329	3	2	theme	few	467:469	arg1	scales					471:476	only few scales	462:476	only few scales	462:476	Experimental measurement of TFEs is challenging, and only few scales were extended to depth-dependent energetic profiles.					
29535329	8	3	from	lipopolysaccharide	1325:1342	arg1	leaflet					1357:1363	the outer leaflet	1347:1363	the outer leaflet	1347:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	1	4	theme	transfer	243:250	arg1	TFEs					267:270	TFEs	267:270	TFEs	267:270	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	4	theme	transfer	243:250	arg1	energies					257:264	accurate transfer free energies	234:264	accurate transfer free energies (TFEs)	234:271	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	6	5	theme	β-barrel	883:890	arg1	MPs					906:908	β-barrel and α-helical MPs	883:908	β-barrel and α-helical MPs	883:908	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	1	6	theme	free	252:255	arg1	TFEs					267:270	TFEs	267:270	TFEs	267:270	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	6	theme	free	252:255	arg1	energies					257:264	accurate transfer free energies	234:264	accurate transfer free energies (TFEs)	234:271	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	7	7	theme	positive-inside	1038:1052	arg1	rules					1055:1059	the 'charge-outside' and 'positive-inside' rules	1012:1059	the 'charge-outside' and 'positive-inside' rules for β-barrels and α-helical proteins	1012:1096	Our data confirm the 'charge-outside' and 'positive-inside' rules for β-barrels and α-helical proteins, respectively.					
29535329	1	8	with	insertion	140:148	arg1	lipids					207:212	lipids	207:212	lipids	207:212	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	8	with	insertion	140:148	arg1	drugs					219:223	drugs	219:223	drugs	219:223	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	8	with	insertion	140:148	arg1	proteins					197:204	other proteins	191:204	other proteins	191:204	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	2	9	theme	Various	274:280	arg1	scales					286:291	Various TFE scales	274:291	Various TFE scales	274:291	Various TFE scales have been derived to quantify the energy required or released to insert an amino acid or protein into the membrane.					
29535329	8	10	theme	lipopolysaccharide	1325:1342	arg1	membranes					1294:1302	gram-negative bacterial outer membranes	1264:1302	gram-negative bacterial outer membranes	1264:1302	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	10	theme	lipopolysaccharide	1325:1342	arg1	existence					1312:1320	the existence	1308:1320	the existence of lipopolysaccharide in the outer leaflet	1308:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	11	theme	outer	1288:1292	arg1	membranes					1294:1302	gram-negative bacterial outer membranes	1264:1302	gram-negative bacterial outer membranes	1264:1302	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	2	12	dep	energy	327:332	arg1	required					334:341	required	334:341	required	334:341	Various TFE scales have been derived to quantify the energy required or released to insert an amino acid or protein into the membrane.					
29535329	2	12	dep	energy	327:332	arg1	released					346:353	released	346:353	released to insert an amino acid or protein into the membrane	346:406	Various TFE scales have been derived to quantify the energy required or released to insert an amino acid or protein into the membrane.					
29535329	6	13	theme	proteins	969:976	arg1	orientation					954:964	orientation	954:964	orientation	954:964	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	6	13	theme	proteins	969:976	arg1	depth					944:948	depth	944:948	depth	944:948	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	6	13	theme	proteins	969:976	arg1	topology					934:941	topology	934:941	topology	934:941	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	6	14	theme	membrane	858:865	arg1	potentials					867:876	asymmetric implicit membrane potentials	838:876	asymmetric implicit membrane potentials	838:876	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	1	15	with	folding	131:137	arg1	lipids					207:212	lipids	207:212	lipids	207:212	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	15	with	folding	131:137	arg1	drugs					219:223	drugs	219:223	drugs	219:223	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	15	with	folding	131:137	arg1	proteins					197:204	other proteins	191:204	other proteins	191:204	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	8	16	contain	have	1148:1151	arg1	profiles					1139:1146	the β-barrel profiles	1126:1146	the β-barrel profiles	1126:1146	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	16	contain	have	1148:1151	arg2	result					1215:1220	a result	1213:1220	a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet	1213:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	16	contain	have	1148:1151	arg2	asymmetry					1161:1169	greater asymmetry	1153:1169	greater asymmetry	1153:1169	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	17	from	leaflet	1357:1363	arg1	membranes					1294:1302	gram-negative bacterial outer membranes	1264:1302	gram-negative bacterial outer membranes	1264:1302	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	17	from	leaflet	1357:1363	arg1	existence					1312:1320	the existence	1308:1320	the existence of lipopolysaccharide in the outer leaflet	1308:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	2	18	theme	amino	368:372	arg1	acid					374:377	an amino acid	365:377	an amino acid	365:377	Various TFE scales have been derived to quantify the energy required or released to insert an amino acid or protein into the membrane.					
29535329	5	19	theme	asymmetry	757:765	arg1	terms					729:733	terms	729:733	terms of lipid composition, asymmetry, and protein content between organisms and organelles	729:819	Furthermore, MPs are tightly coupled to bilayers that are heterogeneous in terms of lipid composition, asymmetry, and protein content between organisms and organelles.					
29535329	6	20	theme	implicit	849:856	arg1	potentials					867:876	asymmetric implicit membrane potentials	838:876	asymmetric implicit membrane potentials	838:876	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	6	21	from	depth	944:948	arg1	membrane					985:992	the membrane	981:992	the membrane	981:992	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	3	22	theme	depth-dependent	495:509	arg1	profiles					521:528	depth-dependent energetic profiles	495:528	depth-dependent energetic profiles	495:528	Experimental measurement of TFEs is challenging, and only few scales were extended to depth-dependent energetic profiles.					
29535329	3	23	theme	Experimental	409:420	arg1	measurement					422:432	Experimental measurement	409:432	Experimental measurement of TFEs	409:440	Experimental measurement of TFEs is challenging, and only few scales were extended to depth-dependent energetic profiles.					
29535329	1	24	with	protein	118:124	arg1	lipids					207:212	lipids	207:212	lipids	207:212	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	24	with	protein	118:124	arg1	drugs					219:223	drugs	219:223	drugs	219:223	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	24	with	protein	118:124	arg1	proteins					197:204	other proteins	191:204	other proteins	191:204	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	6	25	theme	asymmetric	838:847	arg1	potentials					867:876	asymmetric implicit membrane potentials	838:876	asymmetric implicit membrane potentials	838:876	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	9	26	contain	have	1430:1433	arg1	residues					1408:1415	pore-facing residues	1396:1415	pore-facing residues in β-barrels	1396:1428	Our data further suggest that pore-facing residues in β-barrels have a larger contribution to membrane insertion and stability than previously suggested.					
29535329	9	26	contain	have	1430:1433	arg2	contribution					1444:1455	a larger contribution	1435:1455	a larger contribution to membrane insertion and stability than previously suggested	1435:1517	Our data further suggest that pore-facing residues in β-barrels have a larger contribution to membrane insertion and stability than previously suggested.					
29535329	1	27	theme	Modeling	100:107	arg1	MP					127:128	MP	127:128	MP	127:128	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	27	theme	Modeling	100:107	arg1	protein					118:124	Modeling membrane protein	100:124	Modeling membrane protein	100:124	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	4	28	theme	MP	639:640	arg1	structures					642:651	MP structures	639:651	MP structures	639:651	Statistical approaches can be used to derive such potentials; however, this requires a sufficient number of MP structures.					
29535329	4	29	theme	Statistical	531:541	arg1	approaches					543:552	Statistical approaches	531:552	Statistical approaches	531:552	Statistical approaches can be used to derive such potentials; however, this requires a sufficient number of MP structures.					
29535329	6	30	from	orientation	954:964	arg1	membrane					985:992	the membrane	981:992	the membrane	981:992	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	8	31	from	proteins	1200:1207	arg1	ones					1180:1183	the ones	1176:1183	the ones from α-helical proteins	1176:1207	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	1	32	theme	membrane	109:116	arg1	MP					127:128	MP	127:128	MP	127:128	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	32	theme	membrane	109:116	arg1	protein					118:124	Modeling membrane protein	100:124	Modeling membrane protein	100:124	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	33	theme	other	191:195	arg1	proteins					197:204	other proteins	191:204	other proteins	191:204	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	0	34	theme	membrane	33:40	arg1	potentials					42:51	asymmetric membrane potentials	22:51	asymmetric membrane potentials	22:51	Statistically derived asymmetric membrane potentials from α-helical and β-barrel membrane proteins.					
29535329	7	35	theme	charge-outside	1017:1030	arg1	rules					1055:1059	the 'charge-outside' and 'positive-inside' rules	1012:1059	the 'charge-outside' and 'positive-inside' rules for β-barrels and α-helical proteins	1012:1096	Our data confirm the 'charge-outside' and 'positive-inside' rules for β-barrels and α-helical proteins, respectively.					
29535329	3	36	theme	TFEs	437:440	arg1	measurement					422:432	Experimental measurement	409:432	Experimental measurement of TFEs	409:440	Experimental measurement of TFEs is challenging, and only few scales were extended to depth-dependent energetic profiles.					
29535329	0	37	theme	asymmetric	22:31	arg1	potentials					42:51	asymmetric membrane potentials	22:51	asymmetric membrane potentials	22:51	Statistically derived asymmetric membrane potentials from α-helical and β-barrel membrane proteins.					
29535329	9	38	theme	membrane	1460:1467	arg1	insertion					1469:1477	membrane insertion	1460:1477	membrane insertion	1460:1477	Our data further suggest that pore-facing residues in β-barrels have a larger contribution to membrane insertion and stability than previously suggested.					
29535329	2	39	theme	TFE	282:284	arg1	scales					286:291	Various TFE scales	274:291	Various TFE scales	274:291	Various TFE scales have been derived to quantify the energy required or released to insert an amino acid or protein into the membrane.					
29535329	8	40	from	membranes	1294:1302	arg1	leaflet					1357:1363	the outer leaflet	1347:1363	the outer leaflet	1347:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	5	41	theme	lipid	738:742	arg1	composition					744:754	lipid composition	738:754	lipid composition	738:754	Furthermore, MPs are tightly coupled to bilayers that are heterogeneous in terms of lipid composition, asymmetry, and protein content between organisms and organelles.					
29535329	8	42	theme	outer	1351:1355	arg1	leaflet					1357:1363	the outer leaflet	1347:1363	the outer leaflet	1347:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	43	theme	α-helical	1190:1198	arg1	proteins					1200:1207	α-helical proteins	1190:1207	α-helical proteins	1190:1207	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	5	44	theme	composition	744:754	arg1	terms					729:733	terms	729:733	terms of lipid composition, asymmetry, and protein content between organisms and organelles	729:819	Furthermore, MPs are tightly coupled to bilayers that are heterogeneous in terms of lipid composition, asymmetry, and protein content between organisms and organelles.					
29535329	6	45	from	topology	934:941	arg1	membrane					985:992	the membrane	981:992	the membrane	981:992	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	9	46	theme	larger	1437:1442	arg1	contribution					1444:1455	a larger contribution	1435:1455	a larger contribution to membrane insertion and stability than previously suggested	1435:1517	Our data further suggest that pore-facing residues in β-barrels have a larger contribution to membrane insertion and stability than previously suggested.					
29535329	5	47	theme	protein	772:778	arg1	content					780:786	protein content	772:786	protein content	772:786	Furthermore, MPs are tightly coupled to bilayers that are heterogeneous in terms of lipid composition, asymmetry, and protein content between organisms and organelles.					
29535329	8	48	theme	different	1229:1237	arg1	architecture					1248:1259	the different membrane architecture	1225:1259	the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet	1225:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	49	theme	β-barrel	1130:1137	arg1	profiles					1139:1146	the β-barrel profiles	1126:1146	the β-barrel profiles	1126:1146	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	7	50	theme	α-helical	1079:1087	arg1	proteins					1089:1096	α-helical proteins	1079:1096	α-helical proteins	1079:1096	Our data confirm the 'charge-outside' and 'positive-inside' rules for β-barrels and α-helical proteins, respectively.					
29535329	9	51	theme	pore-facing	1396:1406	arg1	residues					1408:1415	pore-facing residues	1396:1415	pore-facing residues in β-barrels	1396:1428	Our data further suggest that pore-facing residues in β-barrels have a larger contribution to membrane insertion and stability than previously suggested.					
29535329	8	52	theme	membranes	1294:1302	arg1	architecture					1248:1259	the different membrane architecture	1225:1259	the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet	1225:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	53	theme	existence	1312:1320	arg1	architecture					1248:1259	the different membrane architecture	1225:1259	the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet	1225:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	0	54	theme	α-helical	58:66	arg1	proteins					90:97	α-helical and β-barrel membrane proteins	58:97	α-helical and β-barrel membrane proteins	58:97	Statistically derived asymmetric membrane potentials from α-helical and β-barrel membrane proteins.					
29535329	9	55	dep	contribution	1444:1455	arg1	suggested					1509:1517	suggested	1509:1517	suggested	1509:1517	Our data further suggest that pore-facing residues in β-barrels have a larger contribution to membrane insertion and stability than previously suggested.					
29535329	5	56	from	terms	729:733	arg1	heterogeneous					712:724	heterogeneous	712:724	heterogeneous	712:724	Furthermore, MPs are tightly coupled to bilayers that are heterogeneous in terms of lipid composition, asymmetry, and protein content between organisms and organelles.					
29535329	8	57	theme	greater	1153:1159	arg1	asymmetry					1161:1169	greater asymmetry	1153:1169	greater asymmetry	1153:1169	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	57	theme	greater	1153:1159	arg1	result					1215:1220	a result	1213:1220	a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet	1213:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	2	58	dep	insert	358:363	arg1	acid					374:377	an amino acid	365:377	an amino acid	365:377	Various TFE scales have been derived to quantify the energy required or released to insert an amino acid or protein into the membrane.					
29535329	8	59	theme	membrane	1239:1246	arg1	architecture					1248:1259	the different membrane architecture	1225:1259	the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet	1225:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	1	60	with	interactions	173:184	arg1	lipids					207:212	lipids	207:212	lipids	207:212	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	60	with	interactions	173:184	arg1	drugs					219:223	drugs	219:223	drugs	219:223	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	60	with	interactions	173:184	arg1	proteins					197:204	other proteins	191:204	other proteins	191:204	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	5	61	from	heterogeneous	712:724	arg1	terms					729:733	terms	729:733	terms of lipid composition, asymmetry, and protein content between organisms and organelles	729:819	Furthermore, MPs are tightly coupled to bilayers that are heterogeneous in terms of lipid composition, asymmetry, and protein content between organisms and organelles.					
29535329	3	62	theme	energetic	511:519	arg1	profiles					521:528	depth-dependent energetic profiles	495:528	depth-dependent energetic profiles	495:528	Experimental measurement of TFEs is challenging, and only few scales were extended to depth-dependent energetic profiles.					
29535329	9	63	from	residues	1408:1415	arg1	β-barrels					1420:1428	β-barrels	1420:1428	β-barrels	1420:1428	Our data further suggest that pore-facing residues in β-barrels have a larger contribution to membrane insertion and stability than previously suggested.					
29535329	8	64	theme	architecture	1248:1259	arg1	asymmetry					1161:1169	greater asymmetry	1153:1169	greater asymmetry	1153:1169	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	64	theme	architecture	1248:1259	arg1	result					1215:1220	a result	1213:1220	a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet	1213:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	8	65	theme	bacterial	1278:1286	arg1	membranes					1294:1302	gram-negative bacterial outer membranes	1264:1302	gram-negative bacterial outer membranes	1264:1302	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	4	66	theme	such	576:579	arg1	potentials					581:590	such potentials	576:590	such potentials	576:590	Statistical approaches can be used to derive such potentials; however, this requires a sufficient number of MP structures.					
29535329	0	67	theme	membrane	81:88	arg1	proteins					90:97	α-helical and β-barrel membrane proteins	58:97	α-helical and β-barrel membrane proteins	58:97	Statistically derived asymmetric membrane potentials from α-helical and β-barrel membrane proteins.					
29535329	8	68	from	existence	1312:1320	arg1	leaflet					1357:1363	the outer leaflet	1347:1363	the outer leaflet	1347:1363	We find that the β-barrel profiles have greater asymmetry than the ones from α-helical proteins, as a result of the different membrane architecture of gram-negative bacterial outer membranes and the existence of lipopolysaccharide in the outer leaflet.					
29535329	4	69	theme	sufficient	618:627	arg1	number					629:634	a sufficient number	616:634	a sufficient number of MP structures	616:651	Statistical approaches can be used to derive such potentials; however, this requires a sufficient number of MP structures.					
29535329	0	70	theme	β-barrel	72:79	arg1	proteins					90:97	α-helical and β-barrel membrane proteins	58:97	α-helical and β-barrel membrane proteins	58:97	Statistically derived asymmetric membrane potentials from α-helical and β-barrel membrane proteins.					
29535329	6	71	theme	α-helical	896:904	arg1	MPs					906:908	β-barrel and α-helical MPs	883:908	β-barrel and α-helical MPs	883:908	Here we derived asymmetric implicit membrane potentials from β-barrel and α-helical MPs and use them to predict topology, depth and orientation of proteins in the membrane.					
29535329	5	72	theme	content	780:786	arg1	terms					729:733	terms	729:733	terms of lipid composition, asymmetry, and protein content between organisms and organelles	729:819	Furthermore, MPs are tightly coupled to bilayers that are heterogeneous in terms of lipid composition, asymmetry, and protein content between organisms and organelles.					
29535329	4	73	theme	structures	642:651	arg1	number					629:634	a sufficient number	616:634	a sufficient number of MP structures	616:651	Statistical approaches can be used to derive such potentials; however, this requires a sufficient number of MP structures.					
29535329	1	74	theme	accurate	234:241	arg1	TFEs					267:270	TFEs	267:270	TFEs	267:270	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
29535329	1	74	theme	accurate	234:241	arg1	energies					257:264	accurate transfer free energies	234:264	accurate transfer free energies (TFEs)	234:271	Modeling membrane protein (MP) folding, insertion, association and their interactions with other proteins, lipids, and drugs requires accurate transfer free energies (TFEs).					
31185241	0	0	theme	pharmacokinetic	79:93	arg1	study					95:99	A pharmacokinetic study	77:99	A pharmacokinetic study	77:99	Potential of di-aldehyde cellulose for sustained release of oxytetracycline: A pharmacokinetic study.					
31185241	3	1	theme	prepared	288:295	arg1	material					305:312	The prepared DAC-OTC material	284:312	The prepared DAC-OTC material	284:312	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	7	2	theme	Swelling	1015:1022	arg1	index					1024:1028	Swelling index	1015:1028	Swelling index of DAC-OTC at different pH and pKa of OTC release	1015:1078	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	6	3	with	pattern	853:859	arg1	improvement					866:876	improvement	866:876	improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞)	866:942	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	6	4	theme	moment	921:926	arg1	curve					928:932	moment curve	921:932	moment curve	921:932	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	3	5	theme	DAC-OTC	297:303	arg1	material					305:312	The prepared DAC-OTC material	284:312	The prepared DAC-OTC material	284:312	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	6	6	theme	compartment	841:851	arg1	pattern					853:859	double compartment pattern	834:859	double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet	834:969	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	1	7	theme	oxytetracycline	157:171	arg1	release					146:152	the in-vivo sustained release	124:152	the in-vivo sustained release of oxytetracycline (OTC) loaded on di-aldehyde cellulose (DAC)	124:215	This study focused on the in-vivo sustained release of oxytetracycline (OTC) loaded on di-aldehyde cellulose (DAC).					
31185241	3	8	theme	different	335:343	arg1	Fourier					400:406	Fourier	400:406	Fourier transforms infrared spectroscopy (FT-IR)	400:447	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	8	theme	different	335:343	arg1	microscopy					382:391	Scanning electron microscopy	364:391	Scanning electron microscopy (SEM)	364:397	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	8	theme	different	335:343	arg1	techniques					345:354	different techniques	335:354	different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer	335:539	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	8	theme	different	335:343	arg1	diffraction					456:466	X-ray diffraction	450:466	X-ray diffraction (XRD)	450:472	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	8	theme	different	335:343	arg1	microscopy					497:506	Transmission electron microscopy	475:506	Transmission electron microscopy (TEM)	475:512	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	8	theme	different	335:343	arg1	analyzer					532:539	particle size analyzer	518:539	particle size analyzer	518:539	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	6	9	theme	double	834:839	arg1	pattern					853:859	double compartment pattern	834:859	double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet	834:969	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	7	10	theme	controlled	1091:1100	arg1	release					1110:1116	controlled in-vivo release	1091:1116	controlled in-vivo release in DAC-OTC composite system	1091:1144	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	4	11	theme	pharmacokinetic	546:560	arg1	studies					562:568	The pharmacokinetic studies	542:568	The pharmacokinetic studies	542:568	The pharmacokinetic studies were performed on DAC-OTC composite system and commercial tablet (COTA).					
31185241	7	12	theme	release	1072:1078	arg1	DAC-OTC					1033:1039	DAC-OTC	1033:1039	DAC-OTC at different pH and pKa of OTC release	1033:1078	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	7	13	from	pH	1054:1055	arg1	DAC-OTC					1033:1039	DAC-OTC	1033:1039	DAC-OTC at different pH and pKa of OTC release	1033:1078	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	7	13	from	pH	1054:1055	arg1	index					1024:1028	Swelling index	1015:1028	Swelling index of DAC-OTC at different pH and pKa of OTC release	1015:1078	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	7	14	theme	DAC-OTC	1121:1127	arg1	system					1139:1144	DAC-OTC composite system	1121:1144	DAC-OTC composite system	1121:1144	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	3	15	theme	X-ray	450:454	arg1	diffraction					456:466	X-ray diffraction	450:466	X-ray diffraction (XRD)	450:472	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	15	theme	X-ray	450:454	arg1	XRD					469:471	XRD	469:471	XRD	469:471	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	7	16	theme	OTC	1068:1070	arg1	release					1072:1078	OTC release	1068:1078	OTC release	1068:1078	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	4	17	theme	DAC-OTC	588:594	arg1	system					606:611	DAC-OTC composite system	588:611	DAC-OTC composite system	588:611	The pharmacokinetic studies were performed on DAC-OTC composite system and commercial tablet (COTA).					
31185241	3	18	theme	electron	488:495	arg1	TEM					509:511	TEM	509:511	TEM	509:511	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	18	theme	electron	488:495	arg1	microscopy					497:506	Transmission electron microscopy	475:506	Transmission electron microscopy (TEM)	475:512	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	6	19	theme	commercial	953:962	arg1	tablet					964:969	the commercial tablet	949:969	the commercial tablet	949:969	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	5	20	theme	higher	718:723	arg1	μghmL-1					759:765	482.8 μghmL-1	753:765	482.8 μghmL-1	753:765	The results of pharmacokinetic studies demonstrated that DAC-OTC exhibited higher area under the curve (AUC) (482.8 μghmL-1) as compared to COTA (90.72 μghmL-1).					
31185241	5	20	theme	higher	718:723	arg1	AUC					747:749	AUC	747:749	AUC	747:749	The results of pharmacokinetic studies demonstrated that DAC-OTC exhibited higher area under the curve (AUC) (482.8 μghmL-1) as compared to COTA (90.72 μghmL-1).					
31185241	5	20	theme	higher	718:723	arg1	area					725:728	higher area	718:728	higher area under the curve (AUC) (482.8 μghmL-1)	718:766	The results of pharmacokinetic studies demonstrated that DAC-OTC exhibited higher area under the curve (AUC) (482.8 μghmL-1) as compared to COTA (90.72 μghmL-1).					
31185241	6	21	theme	composite	813:821	arg1	system					823:828	DAC-OTC composite system	805:828	DAC-OTC composite system	805:828	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	0	22	dep	study	95:99	arg1	Potential					0:8	Potential	0:8	Potential of di-aldehyde cellulose for sustained release of oxytetracycline	0:74	Potential of di-aldehyde cellulose for sustained release of oxytetracycline: A pharmacokinetic study.					
31185241	3	23	theme	infrared	419:426	arg1	FT-IR					442:446	FT-IR	442:446	FT-IR	442:446	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	23	theme	infrared	419:426	arg1	spectroscopy					428:439	infrared spectroscopy	419:439	infrared spectroscopy (FT-IR)	419:447	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	24	theme	Scanning	364:371	arg1	SEM					394:396	SEM	394:396	SEM	394:396	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	24	theme	Scanning	364:371	arg1	microscopy					382:391	Scanning electron microscopy	364:391	Scanning electron microscopy (SEM)	364:397	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	6	25	theme	DAC-OTC	805:811	arg1	system					823:828	DAC-OTC composite system	805:828	DAC-OTC composite system	805:828	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	7	26	theme	in-vivo	1102:1108	arg1	release					1110:1116	controlled in-vivo release	1091:1116	controlled in-vivo release in DAC-OTC composite system	1091:1144	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	3	27	theme	particle	518:525	arg1	analyzer					532:539	particle size analyzer	518:539	particle size analyzer	518:539	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	7	28	theme	covalent	1197:1204	arg1	bond					1219:1222	the covalent and hydrogen bond	1193:1222	bond	1219:1222	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	3	29	theme	electron	373:380	arg1	SEM					394:396	SEM	394:396	SEM	394:396	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	29	theme	electron	373:380	arg1	microscopy					382:391	Scanning electron microscopy	364:391	Scanning electron microscopy (SEM)	364:397	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	30	theme	size	527:530	arg1	analyzer					532:539	particle size analyzer	518:539	particle size analyzer	518:539	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	0	31	theme	cellulose	25:33	arg1	Potential					0:8	Potential	0:8	Potential of di-aldehyde cellulose for sustained release of oxytetracycline	0:74	Potential of di-aldehyde cellulose for sustained release of oxytetracycline: A pharmacokinetic study.					
31185241	8	32	theme	in-vivo	1329:1335	arg1	bioavailability					1337:1351	the in-vivo bioavailability	1325:1351	the in-vivo bioavailability of OTC	1325:1358	These results indicate that di-aldehyde cellulose may improve the in-vivo bioavailability of OTC.					
31185241	2	33	theme	DAC	279:281	arg1	synthesis					266:274	the synthesis	262:274	the synthesis of DAC	262:281	The periodate oxidation method was used for the synthesis of DAC.					
31185241	4	34	theme	composite	596:604	arg1	system					606:611	DAC-OTC composite system	588:611	DAC-OTC composite system	588:611	The pharmacokinetic studies were performed on DAC-OTC composite system and commercial tablet (COTA).					
31185241	0	35	theme	di-aldehyde	13:23	arg1	cellulose					25:33	di-aldehyde cellulose	13:33	di-aldehyde cellulose	13:33	Potential of di-aldehyde cellulose for sustained release of oxytetracycline: A pharmacokinetic study.					
31185241	5	36	theme	pharmacokinetic	658:672	arg1	studies					674:680	pharmacokinetic studies	658:680	pharmacokinetic studies	658:680	The results of pharmacokinetic studies demonstrated that DAC-OTC exhibited higher area under the curve (AUC) (482.8 μghmL-1) as compared to COTA (90.72 μghmL-1).					
31185241	7	37	theme	composite	1129:1137	arg1	system					1139:1144	DAC-OTC composite system	1121:1144	DAC-OTC composite system	1121:1144	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	1	38	theme	di-aldehyde	189:199	arg1	DAC					212:214	DAC	212:214	DAC	212:214	This study focused on the in-vivo sustained release of oxytetracycline (OTC) loaded on di-aldehyde cellulose (DAC).					
31185241	1	38	theme	di-aldehyde	189:199	arg1	cellulose					201:209	di-aldehyde cellulose	189:209	di-aldehyde cellulose (DAC)	189:215	This study focused on the in-vivo sustained release of oxytetracycline (OTC) loaded on di-aldehyde cellulose (DAC).					
31185241	0	39	theme	sustained	39:47	arg1	release					49:55	sustained release	39:55	sustained release of oxytetracycline	39:74	Potential of di-aldehyde cellulose for sustained release of oxytetracycline: A pharmacokinetic study.					
31185241	5	40	theme	studies	674:680	arg1	results					647:653	The results	643:653	The results of pharmacokinetic studies	643:680	The results of pharmacokinetic studies demonstrated that DAC-OTC exhibited higher area under the curve (AUC) (482.8 μghmL-1) as compared to COTA (90.72 μghmL-1).					
31185241	7	41	theme	simultaneous	1166:1177	arg1	occurrence					1179:1188	the simultaneous occurrence	1162:1188	the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose	1162:1260	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	7	42	theme	hydrogen	1210:1217	arg1	bond					1219:1222	the covalent and hydrogen bond	1193:1222	bond	1219:1222	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	6	43	theme	residing	886:893	arg1	MRT					901:903	MRT	901:903	MRT	901:903	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	6	43	theme	residing	886:893	arg1	time					895:898	mean residing time	881:898	mean residing time (MRT)	881:904	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	7	44	theme	different	1044:1052	arg1	pH					1054:1055	different pH	1044:1055	different pH	1044:1055	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	3	45	dep	Fourier	400:406	arg1	transforms					408:417	transforms	408:417	transforms infrared spectroscopy (FT-IR)	408:447	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	6	46	contain	has	830:832	arg1	system					823:828	DAC-OTC composite system	805:828	DAC-OTC composite system	805:828	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	6	46	contain	has	830:832	arg2	pattern					853:859	double compartment pattern	834:859	double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet	834:969	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	6	47	theme	mean	881:884	arg1	MRT					901:903	MRT	901:903	MRT	901:903	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	6	47	theme	mean	881:884	arg1	time					895:898	mean residing time	881:898	mean residing time (MRT)	881:904	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	8	48	theme	OTC	1356:1358	arg1	bioavailability					1337:1351	the in-vivo bioavailability	1325:1351	the in-vivo bioavailability of OTC	1325:1358	These results indicate that di-aldehyde cellulose may improve the in-vivo bioavailability of OTC.					
31185241	7	49	from	pKa	1061:1063	arg1	DAC-OTC					1033:1039	DAC-OTC	1033:1039	DAC-OTC at different pH and pKa of OTC release	1033:1078	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	7	49	from	pKa	1061:1063	arg1	index					1024:1028	Swelling index	1015:1028	Swelling index of DAC-OTC at different pH and pKa of OTC release	1015:1078	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	7	50	theme	DAC-OTC	1033:1039	arg1	index					1024:1028	Swelling index	1015:1028	Swelling index of DAC-OTC at different pH and pKa of OTC release	1015:1078	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	6	51	from	improvement	866:876	arg1	MRT					901:903	MRT	901:903	MRT	901:903	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	6	51	from	improvement	866:876	arg1	AUMC0-∞					935:941	AUMC0-∞	935:941	AUMC0-∞	935:941	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	6	51	from	improvement	866:876	arg1	area					910:913	area	910:913	area under moment curve (AUMC0-∞)	910:942	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	6	51	from	improvement	866:876	arg1	time					895:898	mean residing time	881:898	mean residing time (MRT)	881:904	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	8	52	theme	di-aldehyde	1291:1301	arg1	cellulose					1303:1311	di-aldehyde cellulose	1291:1311	di-aldehyde cellulose	1291:1311	These results indicate that di-aldehyde cellulose may improve the in-vivo bioavailability of OTC.					
31185241	7	53	theme	bond	1219:1222	arg1	occurrence					1179:1188	the simultaneous occurrence	1162:1188	the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose	1162:1260	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	7	54	from	index	1024:1028	arg1	pKa					1061:1063	pKa	1061:1063	pKa	1061:1063	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	7	54	from	index	1024:1028	arg1	pH					1054:1055	different pH	1044:1055	different pH	1044:1055	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	6	55	dep	15.13	980:984	arg1	folds					986:990	folds	986:990	folds higher	986:997	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	7	56	from	release	1110:1116	arg1	system					1139:1144	DAC-OTC composite system	1121:1144	DAC-OTC composite system	1121:1144	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	7	57	theme	di-aldehyde	1240:1250	arg1	cellulose					1252:1260	di-aldehyde cellulose	1240:1260	di-aldehyde cellulose	1240:1260	Swelling index of DAC-OTC at different pH and pKa of OTC release imply that controlled in-vivo release in DAC-OTC composite system could be due to the simultaneous occurrence of the covalent and hydrogen bond between OTC and di-aldehyde cellulose.					
31185241	1	58	theme	in-vivo	128:134	arg1	release					146:152	the in-vivo sustained release	124:152	the in-vivo sustained release of oxytetracycline (OTC) loaded on di-aldehyde cellulose (DAC)	124:215	This study focused on the in-vivo sustained release of oxytetracycline (OTC) loaded on di-aldehyde cellulose (DAC).					
31185241	2	59	theme	oxidation	232:240	arg1	method					242:247	The periodate oxidation method	218:247	The periodate oxidation method	218:247	The periodate oxidation method was used for the synthesis of DAC.					
31185241	1	60	theme	sustained	136:144	arg1	release					146:152	the in-vivo sustained release	124:152	the in-vivo sustained release of oxytetracycline (OTC) loaded on di-aldehyde cellulose (DAC)	124:215	This study focused on the in-vivo sustained release of oxytetracycline (OTC) loaded on di-aldehyde cellulose (DAC).					
31185241	4	61	theme	commercial	617:626	arg1	tablet					628:633	commercial tablet	617:633	commercial tablet (COTA)	617:640	The pharmacokinetic studies were performed on DAC-OTC composite system and commercial tablet (COTA).					
31185241	4	61	theme	commercial	617:626	arg1	COTA					636:639	COTA	636:639	COTA	636:639	The pharmacokinetic studies were performed on DAC-OTC composite system and commercial tablet (COTA).					
31185241	6	62	dep	has	830:832	arg1	2.8					972:974	2.8	972:974	2.8	972:974	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	6	62	dep	has	830:832	arg1	15.13					980:984	15.13	980:984	15.13	980:984	DAC-OTC composite system has double compartment pattern with improvement in mean residing time (MRT) and area under moment curve (AUMC0-∞) than the commercial tablet (2.8 and 15.13 folds higher, respectively).					
31185241	0	63	theme	oxytetracycline	60:74	arg1	release					49:55	sustained release	39:55	sustained release of oxytetracycline	39:74	Potential of di-aldehyde cellulose for sustained release of oxytetracycline: A pharmacokinetic study.					
31185241	2	64	theme	periodate	222:230	arg1	method					242:247	The periodate oxidation method	218:247	The periodate oxidation method	218:247	The periodate oxidation method was used for the synthesis of DAC.					
31185241	3	65	theme	Transmission	475:486	arg1	TEM					509:511	TEM	509:511	TEM	509:511	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	3	65	theme	Transmission	475:486	arg1	microscopy					497:506	Transmission electron microscopy	475:506	Transmission electron microscopy (TEM)	475:512	The prepared DAC-OTC material was characterized by different techniques such as Scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy (FT-IR), X-ray diffraction (XRD), Transmission electron microscopy (TEM) and particle size analyzer.					
31185241	2	66	used	used	253:256	arg2	method					242:247	The periodate oxidation method	218:247	The periodate oxidation method	218:247	The periodate oxidation method was used for the synthesis of DAC.					
29488965	0	0	theme	activated	77:85	arg1	sludge					87:92	the activated sludge	73:92	the activated sludge	73:92	The model binary/ternary mixtures for actual EPS solution extracted from the activated sludge in MBR using dead-end membrane filtration cell.					
29488965	2	1	dep	parameters	522:531	arg1	volume					554:559	cumulative filtrate volume	534:559	cumulative filtrate volume (CFV)	534:565	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	1	dep	parameters	522:531	arg1	resistance					582:591	specific cake resistance	568:591	specific cake resistance (αc)	568:596	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	1	dep	parameters	522:531	arg1	R					613:613	R	613:613	R	613:613	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	1	dep	parameters	522:531	arg1	parameters					522:531	Three target parameters	509:531	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R))	509:615	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	1	dep	parameters	522:531	arg1	CFV					562:564	CFV	562:564	CFV	562:564	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	1	dep	parameters	522:531	arg1	αc					594:595	αc	594:595	αc	594:595	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	1	dep	parameters	522:531	arg1	rejection					602:610	rejection	602:610	rejection (R)	602:614	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	1	2	theme	humic	492:496	arg1	HA					504:505	HA	504:505	HA	504:505	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	2	theme	humic	492:496	arg1	acid					498:501	humic acid	492:501	humic acid (HA)	492:506	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	5	3	theme	binary	1250:1255	arg1	solution					1257:1264	the binary solution	1246:1264	the binary solution for imitating the filterability of actual EPS solution	1246:1319	This indicated that the ternary solution demonstrated a more accurate estimation than the binary solution for imitating the filterability of actual EPS solution.					
29488965	4	4	theme	binary	1077:1082	arg1	mg·L-1					1115:1120	SA/BSA = 140.4/50.35 mg·L-1	1094:1120	SA/BSA = 140.4/50.35 mg·L-1	1094:1120	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	4	4	theme	binary	1077:1082	arg1	solution					1084:1091	the most suitable binary solution	1059:1091	the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1)	1059:1121	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	2	5	theme	filtrate	545:552	arg1	CFV					562:564	CFV	562:564	CFV	562:564	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	5	theme	filtrate	545:552	arg1	volume					554:559	cumulative filtrate volume	534:559	cumulative filtrate volume (CFV)	534:565	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	5	theme	filtrate	545:552	arg1	parameters					522:531	Three target parameters	509:531	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R))	509:615	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	3	6	theme	ternary	739:745	arg1	mixture					747:753	ternary mixture	739:753	ternary mixture	739:753	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	6	7	theme	target	1436:1441	arg1	parameters					1443:1452	three target parameters	1430:1452	three target parameters in practice applications	1430:1477	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	2	8	theme	cumulative	534:543	arg1	CFV					562:564	CFV	562:564	CFV	562:564	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	8	theme	cumulative	534:543	arg1	volume					554:559	cumulative filtrate volume	534:559	cumulative filtrate volume (CFV)	534:565	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	8	theme	cumulative	534:543	arg1	parameters					522:531	Three target parameters	509:531	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R))	509:615	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	9	theme	cake	577:580	arg1	resistance					582:591	specific cake resistance	568:591	specific cake resistance (αc)	568:596	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	9	theme	cake	577:580	arg1	αc					594:595	αc	594:595	αc	594:595	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	9	theme	cake	577:580	arg1	parameters					522:531	Three target parameters	509:531	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R))	509:615	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	3	10	theme	influence	709:717	arg1	degree					699:704	degree	699:704	degree of influence on CFV, αc and R in ternary mixture	699:753	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	1	11	theme	dead-end	325:332	arg1	cell					344:347	a dead-end unstirred cell	323:347	a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes	323:393	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	4	12	theme	mixture	901:907	arg1	SA/BSA/HA					913:921	SA/BSA/HA	913:921	SA/BSA/HA = 285.1/150.1/10.2 mg·L-1	913:947	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	4	12	theme	mixture	901:907	arg1	composition					878:888	the composition	874:888	the composition of ternary mixture	874:907	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	1	13	theme	sodium	439:444	arg1	SA					456:457	SA	456:457	SA	456:457	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	13	theme	sodium	439:444	arg1	alginate					446:453	sodium alginate	439:453	sodium alginate (SA)	439:458	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	2	14	theme	components	656:665	arg1	roles					639:643	the roles	635:643	the roles of mixture components	635:665	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	4	15	theme	SA/BSA	1094:1099	arg1	mg·L-1					1115:1120	SA/BSA = 140.4/50.35 mg·L-1	1094:1120	SA/BSA = 140.4/50.35 mg·L-1	1094:1120	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	4	15	theme	SA/BSA	1094:1099	arg1	solution					1084:1091	the most suitable binary solution	1059:1091	the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1)	1059:1121	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	1	16	theme	unstirred	334:342	arg1	cell					344:347	a dead-end unstirred cell	323:347	a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes	323:393	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	6	17	from	applications	1466:1477	arg1	terms					1421:1425	terms	1421:1425	terms of three target parameters in practice applications	1421:1477	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	2	18	theme	specific	568:575	arg1	resistance					582:591	specific cake resistance	568:591	specific cake resistance (αc)	568:596	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	18	theme	specific	568:575	arg1	αc					594:595	αc	594:595	αc	594:595	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	18	theme	specific	568:575	arg1	parameters					522:531	Three target parameters	509:531	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R))	509:615	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	19	theme	mixture	648:654	arg1	components					656:665	mixture components	648:665	mixture components	648:665	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	5	20	theme	accurate	1221:1228	arg1	estimation					1230:1239	a more accurate estimation	1214:1239	a more accurate estimation than the binary solution for imitating the filterability of actual EPS solution	1214:1319	This indicated that the ternary solution demonstrated a more accurate estimation than the binary solution for imitating the filterability of actual EPS solution.					
29488965	3	21	from	influence	709:717	arg1	R					734:734	R	734:734	R	734:734	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	21	from	influence	709:717	arg1	αc					727:728	αc	727:728	αc	727:728	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	21	from	influence	709:717	arg1	CFV					722:724	CFV	722:724	CFV	722:724	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	6	22	theme	actual	1398:1403	arg1	solution					1409:1416	the actual EPS solution	1394:1416	the actual EPS solution in terms of three target parameters in practice applications	1394:1477	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	0	23	theme	model	4:8	arg1	mixtures					25:32	The model binary/ternary mixtures	0:32	The model binary/ternary mixtures for actual EPS solution	0:56	The model binary/ternary mixtures for actual EPS solution extracted from the activated sludge in MBR using dead-end membrane filtration cell.					
29488965	3	24	from	αc	727:728	arg1	degree					699:704	degree	699:704	degree of influence on CFV, αc and R in ternary mixture	699:753	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	24	from	αc	727:728	arg1	mixture					747:753	ternary mixture	739:753	ternary mixture	739:753	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	6	25	from	solution	1409:1416	arg1	terms					1421:1425	terms	1421:1425	terms of three target parameters in practice applications	1421:1477	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	1	26	theme	model	161:165	arg1	solution					167:174	a model solution	159:174	a model solution	159:174	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	3	27	dep	HA	832:833	arg1	%					854:854	3.8%	851:854	3.8%	851:854	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	27	dep	HA	832:833	arg1	%					839:839	0.3%	836:839	0.3%	836:839	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	27	dep	HA	832:833	arg1	%					845:845	4.1%	842:845	4.1%	842:845	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	4	28	dep	=	923:923	arg1	mg·L-1					942:947	285.1/150.1/10.2 mg·L-1	925:947	285.1/150.1/10.2 mg·L-1	925:947	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	5	29	theme	actual	1301:1306	arg1	solution					1312:1319	actual EPS solution	1301:1319	actual EPS solution	1301:1319	This indicated that the ternary solution demonstrated a more accurate estimation than the binary solution for imitating the filterability of actual EPS solution.					
29488965	1	30	theme	filterability	257:269	arg1	behavior					271:278	filterability behavior	257:278	filterability behavior	257:278	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	0	31	theme	membrane	116:123	arg1	cell					136:139	dead-end membrane filtration cell	107:139	dead-end membrane filtration cell	107:139	The model binary/ternary mixtures for actual EPS solution extracted from the activated sludge in MBR using dead-end membrane filtration cell.					
29488965	3	32	dep	SA	759:760	arg1	%					767:767	94.5%	763:767	94.5%	763:767	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	32	dep	SA	759:760	arg1	%					774:774	85.6%	770:774	85.6%	770:774	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	32	dep	SA	759:760	arg1	%					784:784	88.2%	780:784	88.2%	780:784	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	33	from	R	734:734	arg1	degree					699:704	degree	699:704	degree of influence on CFV, αc and R in ternary mixture	699:753	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	33	from	R	734:734	arg1	mixture					747:753	ternary mixture	739:753	ternary mixture	739:753	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	34	dep	BSA	803:805	arg1	%					811:811	5.2%	808:811	5.2%	808:811	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	34	dep	BSA	803:805	arg1	%					818:818	10.3%	814:818	10.3%	814:818	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	34	dep	BSA	803:805	arg1	%					827:827	8.0%	824:827	8.0%	824:827	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	5	35	theme	solution	1312:1319	arg1	filterability					1284:1296	the filterability	1280:1296	the filterability of actual EPS solution	1280:1319	This indicated that the ternary solution demonstrated a more accurate estimation than the binary solution for imitating the filterability of actual EPS solution.					
29488965	0	36	theme	binary/ternary	10:23	arg1	mixtures					25:32	The model binary/ternary mixtures	0:32	The model binary/ternary mixtures for actual EPS solution	0:56	The model binary/ternary mixtures for actual EPS solution extracted from the activated sludge in MBR using dead-end membrane filtration cell.					
29488965	1	37	theme	behavior	271:278	arg1	terms					248:252	terms	248:252	terms of filterability behavior	248:278	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	0	38	theme	dead-end	107:114	arg1	cell					136:139	dead-end membrane filtration cell	107:139	dead-end membrane filtration cell	107:139	The model binary/ternary mixtures for actual EPS solution extracted from the activated sludge in MBR using dead-end membrane filtration cell.					
29488965	1	39	theme	0.1	354:356	arg1	μm					358:359	μm	358:359	μm	358:359	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	0	40	theme	actual	38:43	arg1	solution					49:56	actual EPS solution	38:56	actual EPS solution	38:56	The model binary/ternary mixtures for actual EPS solution extracted from the activated sludge in MBR using dead-end membrane filtration cell.					
29488965	4	41	theme	suitable	1068:1075	arg1	mg·L-1					1115:1120	SA/BSA = 140.4/50.35 mg·L-1	1094:1120	SA/BSA = 140.4/50.35 mg·L-1	1094:1120	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	4	41	theme	suitable	1068:1075	arg1	solution					1084:1091	the most suitable binary solution	1059:1091	the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1)	1059:1121	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	3	42	from	CFV	722:724	arg1	degree					699:704	degree	699:704	degree of influence on CFV, αc and R in ternary mixture	699:753	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	42	from	CFV	722:724	arg1	mixture					747:753	ternary mixture	739:753	ternary mixture	739:753	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	1	43	theme	μm	358:359	arg1	membranes					385:393	0.1 μm polyvinylidene fluoride membranes	354:393	0.1 μm polyvinylidene fluoride membranes	354:393	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	44	theme	actual	188:193	arg1	EPS					231:233	EPS	231:233	EPS	231:233	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	44	theme	actual	188:193	arg1	substances					219:228	actual extracellular polymeric substances	188:228	actual extracellular polymeric substances (EPS) solution	188:243	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	4	45	theme	corresponding	1034:1046	arg1	values					1048:1053	the corresponding values	1030:1053	the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1)	1030:1121	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	4	45	theme	corresponding	1034:1046	arg1	%					1131:1131	-12%	1128:1131	-12%	1128:1131	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	0	46	theme	filtration	125:134	arg1	cell					136:139	dead-end membrane filtration cell	107:139	dead-end membrane filtration cell	107:139	The model binary/ternary mixtures for actual EPS solution extracted from the activated sludge in MBR using dead-end membrane filtration cell.					
29488965	1	47	theme	polyvinylidene	361:374	arg1	membranes					385:393	0.1 μm polyvinylidene fluoride membranes	354:393	0.1 μm polyvinylidene fluoride membranes	354:393	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	48	theme	bovine	461:466	arg1	albumin					474:480	bovine serum albumin	461:480	bovine serum albumin (BSA)	461:486	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	48	theme	bovine	461:466	arg1	BSA					483:485	BSA	483:485	BSA	483:485	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	49	theme	extracellular	195:207	arg1	EPS					231:233	EPS	231:233	EPS	231:233	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	49	theme	extracellular	195:207	arg1	substances					219:228	actual extracellular polymeric substances	188:228	actual extracellular polymeric substances (EPS) solution	188:243	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	50	theme	fluoride	376:383	arg1	membranes					385:393	0.1 μm polyvinylidene fluoride membranes	354:393	0.1 μm polyvinylidene fluoride membranes	354:393	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	51	theme	serum	468:472	arg1	albumin					474:480	bovine serum albumin	461:480	bovine serum albumin (BSA)	461:486	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	51	theme	serum	468:472	arg1	BSA					483:485	BSA	483:485	BSA	483:485	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	0	52	theme	EPS	45:47	arg1	solution					49:56	actual EPS solution	38:56	actual EPS solution	38:56	The model binary/ternary mixtures for actual EPS solution extracted from the activated sludge in MBR using dead-end membrane filtration cell.					
29488965	2	53	theme	target	515:520	arg1	resistance					582:591	specific cake resistance	568:591	specific cake resistance (αc)	568:596	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	53	theme	target	515:520	arg1	volume					554:559	cumulative filtrate volume	534:559	cumulative filtrate volume (CFV)	534:565	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	53	theme	target	515:520	arg1	rejection					602:610	rejection	602:610	rejection (R)	602:614	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	2	53	theme	target	515:520	arg1	parameters					522:531	Three target parameters	509:531	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R))	509:615	Three target parameters (cumulative filtrate volume (CFV), specific cake resistance (αc) and rejection (R)) were compared and the roles of mixture components were investigated.					
29488965	5	54	theme	ternary	1184:1190	arg1	solution					1192:1199	the ternary solution	1180:1199	the ternary solution	1180:1199	This indicated that the ternary solution demonstrated a more accurate estimation than the binary solution for imitating the filterability of actual EPS solution.					
29488965	4	55	theme	=	923:923	arg1	SA/BSA/HA					913:921	SA/BSA/HA	913:921	SA/BSA/HA = 285.1/150.1/10.2 mg·L-1	913:947	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	4	55	theme	=	923:923	arg1	composition					878:888	the composition	874:888	the composition of ternary mixture	874:907	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	6	56	theme	ternary	1337:1343	arg1	mixture					1345:1351	the ternary mixture	1333:1351	the ternary mixture	1333:1351	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	1	57	with	cell	344:347	arg1	membranes					385:393	0.1 μm polyvinylidene fluoride membranes	354:393	0.1 μm polyvinylidene fluoride membranes	354:393	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	58	theme	experiments	293:303	arg1	series					283:288	a series	281:288	a series of experiments	281:303	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	3	59	from	degree	699:704	arg1	R					734:734	R	734:734	R	734:734	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	59	from	degree	699:704	arg1	αc					727:728	αc	727:728	αc	727:728	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	3	59	from	degree	699:704	arg1	CFV					722:724	CFV	722:724	CFV	722:724	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
29488965	6	60	theme	practice	1457:1464	arg1	applications					1466:1477	practice applications	1457:1477	practice applications	1457:1477	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	1	61	theme	polymeric	209:217	arg1	EPS					231:233	EPS	231:233	EPS	231:233	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	61	theme	polymeric	209:217	arg1	substances					219:228	actual extracellular polymeric substances	188:228	actual extracellular polymeric substances (EPS) solution	188:243	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	6	62	from	terms	1421:1425	arg1	applications					1466:1477	practice applications	1457:1477	practice applications	1457:1477	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	1	63	theme	binary/ternary	401:414	arg1	mixtures					416:423	binary/ternary mixtures	401:423	binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA)	401:506	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	1	64	theme	substances	219:228	arg1	solution					236:243	actual extracellular polymeric substances (EPS) solution	188:243	actual extracellular polymeric substances (EPS) solution	188:243	In order to find a model solution to simulate actual extracellular polymeric substances (EPS) solution in terms of filterability behavior, a series of experiments were conducted in a dead-end unstirred cell with 0.1 μm polyvinylidene fluoride membranes using binary/ternary mixtures consisting of sodium alginate (SA), bovine serum albumin (BSA) and humic acid (HA).					
29488965	6	65	from	parameters	1443:1452	arg1	applications					1466:1477	practice applications	1457:1477	practice applications	1457:1477	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	6	66	theme	parameters	1443:1452	arg1	terms					1421:1425	terms	1421:1425	terms of three target parameters in practice applications	1421:1477	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	4	67	theme	=	1101:1101	arg1	mg·L-1					1115:1120	SA/BSA = 140.4/50.35 mg·L-1	1094:1120	SA/BSA = 140.4/50.35 mg·L-1	1094:1120	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	4	67	theme	=	1101:1101	arg1	solution					1084:1091	the most suitable binary solution	1059:1091	the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1)	1059:1121	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	6	68	theme	EPS	1405:1407	arg1	solution					1409:1416	the actual EPS solution	1394:1416	the actual EPS solution in terms of three target parameters in practice applications	1394:1477	Therefore, the ternary mixture could be employed efficiently to replace the actual EPS solution in terms of three target parameters in practice applications.					
29488965	5	69	theme	EPS	1308:1310	arg1	solution					1312:1319	actual EPS solution	1301:1319	actual EPS solution	1301:1319	This indicated that the ternary solution demonstrated a more accurate estimation than the binary solution for imitating the filterability of actual EPS solution.					
29488965	4	70	theme	ternary	893:899	arg1	mixture					901:907	ternary mixture	893:907	ternary mixture	893:907	Meanwhile, when the composition of ternary mixture was SA/BSA/HA = 285.1/150.1/10.2 mg·L-1, the deviation for CFV, αc and R was 7.65%, 19.6% and 7.27%, respectively, while the corresponding values for the most suitable binary solution (SA/BSA = 140.4/50.35 mg·L-1) were -12%, 1% and 164% respectively.					
29488965	3	71	theme	degree	699:704	arg1	order					690:694	The order	686:694	The order of degree of influence on CFV, αc and R in ternary mixture	686:753	The order of degree of influence on CFV, αc and R in ternary mixture was SA (94.5%, 85.6% and 88.2%, respectively) > BSA (5.2%, 10.3% and 8.0%) > HA (0.3%, 4.1% and 3.8%).					
30797481	8	0	theme	acid	913:916	arg1	substitution					918:929	the single amino acid substitution	896:929	the single amino acid substitution	896:929	The best performing mutant was K291E, for which the single amino acid substitution led to a 3.5-fold increase in kcat/Km.					
30797481	8	1	theme	performing	857:866	arg1	mutant					868:873	The best performing mutant	848:873	The best performing mutant	848:873	The best performing mutant was K291E, for which the single amino acid substitution led to a 3.5-fold increase in kcat/Km.					
30797481	8	1	theme	performing	857:866	arg1	K291E					879:883	K291E	879:883	K291E	879:883	The best performing mutant was K291E, for which the single amino acid substitution led to a 3.5-fold increase in kcat/Km.					
30797481	5	2	theme	acid	303:306	arg1	sequence					308:315	The deduced amino acid sequence	285:315	The deduced amino acid sequence	285:315	The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26.					
30797481	6	3	with	enzyme	571:576	arg1	activity					597:604	high specific activity	583:604	high specific activity	583:604	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	8	4	from	increase	949:956	arg1	kcat/Km					961:967	kcat/Km	961:967	kcat/Km	961:967	The best performing mutant was K291E, for which the single amino acid substitution led to a 3.5-fold increase in kcat/Km.					
30797481	10	5	theme	conformational	1178:1191	arg1	change					1193:1198	a slight conformational change	1169:1198	a slight conformational change	1169:1198	Structural-functional analysis indicated that a slight conformational change could dramatically affect certain enzyme characteristics.					
30797481	6	6	theme	specific	475:482	arg1	activity					484:491	a specific activity	473:491	a specific activity of 2802 U/mg	473:504	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	9	7	dep	Mutants	970:976	arg1	Mutants					970:976	Mutants	970:976	Mutants Q112R and L211I	970:992	Mutants Q112R and L211I also exhibited an apparently increased kcat/Km towards konjac glucomannan, approximately 200% and 80% improvement, respectively.					
30797481	9	7	dep	Mutants	970:976	arg1	L211I					988:992	L211I	988:992	L211I	988:992	Mutants Q112R and L211I also exhibited an apparently increased kcat/Km towards konjac glucomannan, approximately 200% and 80% improvement, respectively.					
30797481	9	7	dep	Mutants	970:976	arg1	Q112R					978:982	Q112R	978:982	Q112R	978:982	Mutants Q112R and L211I also exhibited an apparently increased kcat/Km towards konjac glucomannan, approximately 200% and 80% improvement, respectively.					
30797481	10	8	theme	enzyme	1234:1239	arg1	characteristics					1241:1255	certain enzyme characteristics	1226:1255	certain enzyme characteristics	1226:1255	Structural-functional analysis indicated that a slight conformational change could dramatically affect certain enzyme characteristics.					
30797481	10	9	theme	slight	1171:1176	arg1	change					1193:1198	a slight conformational change	1169:1198	a slight conformational change	1169:1198	Structural-functional analysis indicated that a slight conformational change could dramatically affect certain enzyme characteristics.					
30797481	12	10	theme	new	1408:1410	arg1	findings					1412:1419	These new findings	1402:1419	These new findings	1402:1419	These new findings will help promote the development of industrially useful β-mannanase, with both good thermal stability and high specific activity.					
30797481	3	11	theme	Bacillus	188:195	arg1	WB800					206:210	Bacillus subtilis WB800	188:210	Bacillus subtilis WB800	188:210	MK-2 and expressed in Bacillus subtilis WB800.					
30797481	8	12	theme	3.5-fold	940:947	arg1	increase					949:956	a 3.5-fold increase	938:956	a 3.5-fold increase in kcat/Km	938:967	The best performing mutant was K291E, for which the single amino acid substitution led to a 3.5-fold increase in kcat/Km.					
30797481	5	13	from	subtilis	382:389	arg1	β-mannanase					356:366	the β-mannanase	352:366	the β-mannanase from Bacillus subtilis	352:389	The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26.					
30797481	6	14	theme	molecular	650:658	arg1	engineering					660:670	molecular engineering	650:670	molecular engineering of Bman26	650:680	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	5	15	theme	high	324:327	arg1	identity					338:345	high sequence identity	324:345	high sequence identity	324:345	The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26.					
30797481	6	16	dep	Bacillus	725:732	arg1	subtilis					734:741	subtilis	734:741	subtilis	734:741	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	11	17	theme	thermal	1383:1389	arg1	stability					1391:1399	the enzyme's thermal stability	1370:1399	the enzyme's thermal stability	1370:1399	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	2	18	theme	β-mannanase	120:130	arg1	gene					132:135	A β-mannanase gene	118:135	A β-mannanase gene	118:135	A β-mannanase gene was cloned from Bacillus sp.					
30797481	11	19	located	found	1331:1335	arg2	Gly88-Leu212-Lys288					1295:1313	Gly88-Leu212-Lys288	1295:1313	Gly88-Leu212-Lys288	1295:1313	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	11	19	located	found	1331:1335	arg1	addition					1261:1268	addition	1261:1268	addition	1261:1268	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	11	19	located	found	1331:1335	arg2	sites					1288:1292	three amino acid sites	1271:1292	three amino acid sites (Gly88-Leu212-Lys288) in Bman26	1271:1324	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	10	20	theme	certain	1226:1232	arg1	characteristics					1241:1255	certain enzyme characteristics	1226:1255	certain enzyme characteristics	1226:1255	Structural-functional analysis indicated that a slight conformational change could dramatically affect certain enzyme characteristics.					
30797481	11	21	theme	acid	1283:1286	arg1	sites					1288:1292	three amino acid sites	1271:1292	three amino acid sites (Gly88-Leu212-Lys288) in Bman26	1271:1324	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	11	21	theme	acid	1283:1286	arg1	Gly88-Leu212-Lys288					1295:1313	Gly88-Leu212-Lys288	1295:1313	Gly88-Leu212-Lys288	1295:1313	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	6	22	contain	had	469:471	arg1	enzyme					462:467	The purified recombinant enzyme	437:467	The purified recombinant enzyme	437:467	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	6	22	contain	had	469:471	arg2	activity					484:491	a specific activity	473:491	a specific activity of 2802 U/mg	473:504	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	6	23	theme	industrial	625:634	arg1	applications					636:647	its industrial applications	621:647	its industrial applications	621:647	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	4	24	from	bp	254:255	arg1	length					260:265	length	260:265	length	260:265	The ORF of the β-mannanase gene was 1104 bp in length, encoding 367 aa.					
30797481	3	25	dep	Bacillus	188:195	arg1	subtilis					197:204	subtilis	197:204	subtilis	197:204	MK-2 and expressed in Bacillus subtilis WB800.					
30797481	7	26	theme	positive	756:763	arg1	mutants					765:771	Three positive mutants	750:771	Three positive mutants with substantially improved specific activities	750:819	Three positive mutants with substantially improved specific activities were selected and studied.					
30797481	6	27	theme	Bacillus	725:732	arg1	WB800					743:747	Bacillus subtilis WB800	725:747	Bacillus subtilis WB800	725:747	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	5	28	theme	glycosyl	407:414	arg1	family					426:431	glycosyl hydrolase family 26	407:434	glycosyl hydrolase family 26	407:434	The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26.					
30797481	11	29	theme	amino	1277:1281	arg1	sites					1288:1292	three amino acid sites	1271:1292	three amino acid sites (Gly88-Leu212-Lys288) in Bman26	1271:1324	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	11	29	theme	amino	1277:1281	arg1	Gly88-Leu212-Lys288					1295:1313	Gly88-Leu212-Lys288	1295:1313	Gly88-Leu212-Lys288	1295:1313	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	11	30	from	sites	1288:1292	arg1	Bman26					1319:1324	Bman26	1319:1324	Bman26	1319:1324	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	6	31	theme	recombinant	450:460	arg1	enzyme					462:467	The purified recombinant enzyme	437:467	The purified recombinant enzyme	437:467	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	7	32	theme	improved	792:799	arg1	activities					810:819	substantially improved specific activities	778:819	substantially improved specific activities	778:819	Three positive mutants with substantially improved specific activities were selected and studied.					
30797481	1	33	theme	directed	76:83	arg1	evolution					85:93	its directed evolution	72:93	its directed evolution by random mutagenesis	72:115	MK-2 and its directed evolution by random mutagenesis.					
30797481	5	34	theme	hydrolase	416:424	arg1	family					426:431	glycosyl hydrolase family 26	407:434	glycosyl hydrolase family 26	407:434	The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26.					
30797481	9	35	theme	konjac	1049:1054	arg1	glucomannan					1056:1066	konjac glucomannan	1049:1066	konjac glucomannan	1049:1066	Mutants Q112R and L211I also exhibited an apparently increased kcat/Km towards konjac glucomannan, approximately 200% and 80% improvement, respectively.					
30797481	8	36	theme	single	900:905	arg1	substitution					918:929	the single amino acid substitution	896:929	the single amino acid substitution	896:929	The best performing mutant was K291E, for which the single amino acid substitution led to a 3.5-fold increase in kcat/Km.					
30797481	5	37	theme	deduced	289:295	arg1	sequence					308:315	The deduced amino acid sequence	285:315	The deduced amino acid sequence	285:315	The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26.					
30797481	6	38	theme	purified	441:448	arg1	enzyme					462:467	The purified recombinant enzyme	437:467	The purified recombinant enzyme	437:467	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	4	39	theme	β-mannanase	228:238	arg1	gene					240:243	the β-mannanase gene	224:243	the β-mannanase gene	224:243	The ORF of the β-mannanase gene was 1104 bp in length, encoding 367 aa.					
30797481	0	40	theme	β-mannanase	28:38	arg1	gene					40:43	a β-mannanase gene	26:43	a β-mannanase gene	26:43	Cloning and expression of a β-mannanase gene from Bacillus sp.					
30797481	5	41	theme	amino	297:301	arg1	sequence					308:315	The deduced amino acid sequence	285:315	The deduced amino acid sequence	285:315	The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26.					
30797481	2	42	theme	Bacillus	153:160	arg1	sp					162:163	Bacillus sp	153:163	Bacillus sp	153:163	A β-mannanase gene was cloned from Bacillus sp.					
30797481	4	43	theme	gene	240:243	arg1	bp					254:255	1104 bp	249:255	1104 bp	249:255	The ORF of the β-mannanase gene was 1104 bp in length, encoding 367 aa.					
30797481	4	43	theme	gene	240:243	arg1	ORF					217:219	The ORF	213:219	The ORF of the β-mannanase gene	213:243	The ORF of the β-mannanase gene was 1104 bp in length, encoding 367 aa.					
30797481	6	44	theme	optimum	520:526	arg1	activity					528:535	optimum activity	520:535	optimum activity	520:535	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	11	45	theme	close	1345:1349	arg1	relationships					1351:1363	close relationships	1345:1363	close relationships	1345:1363	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	0	46	from	sp	59:60	arg1	expression					12:21	expression	12:21	expression	12:21	Cloning and expression of a β-mannanase gene from Bacillus sp.					
30797481	0	46	from	sp	59:60	arg1	Cloning					0:6	Cloning	0:6	Cloning	0:6	Cloning and expression of a β-mannanase gene from Bacillus sp.					
30797481	12	47	theme	thermal	1506:1512	arg1	stability					1514:1522	good thermal stability	1501:1522	good thermal stability	1501:1522	These new findings will help promote the development of industrially useful β-mannanase, with both good thermal stability and high specific activity.					
30797481	6	48	theme	random	703:708	arg1	mutagenesis					710:720	random mutagenesis	703:720	random mutagenesis in Bacillus subtilis WB800	703:747	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	12	49	theme	high	1528:1531	arg1	activity					1542:1549	high specific activity	1528:1549	high specific activity	1528:1549	These new findings will help promote the development of industrially useful β-mannanase, with both good thermal stability and high specific activity.					
30797481	0	50	theme	gene	40:43	arg1	expression					12:21	expression	12:21	expression	12:21	Cloning and expression of a β-mannanase gene from Bacillus sp.					
30797481	0	50	theme	gene	40:43	arg1	Cloning					0:6	Cloning	0:6	Cloning	0:6	Cloning and expression of a β-mannanase gene from Bacillus sp.					
30797481	11	51	contain	have	1340:1343	arg1	sites					1288:1292	three amino acid sites	1271:1292	three amino acid sites (Gly88-Leu212-Lys288) in Bman26	1271:1324	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	11	51	contain	have	1340:1343	arg1	Gly88-Leu212-Lys288					1295:1313	Gly88-Leu212-Lys288	1295:1313	Gly88-Leu212-Lys288	1295:1313	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	11	51	contain	have	1340:1343	arg2	relationships					1351:1363	close relationships	1345:1363	close relationships	1345:1363	In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships with the enzyme's thermal stability.					
30797481	9	52	theme	80	1092:1093	arg1	%					1094:1094	%	1094:1094	%	1094:1094	Mutants Q112R and L211I also exhibited an apparently increased kcat/Km towards konjac glucomannan, approximately 200% and 80% improvement, respectively.					
30797481	1	53	theme	random	98:103	arg1	mutagenesis					105:115	random mutagenesis	98:115	random mutagenesis	98:115	MK-2 and its directed evolution by random mutagenesis.					
30797481	6	54	theme	specific	588:595	arg1	activity					597:604	high specific activity	583:604	high specific activity	583:604	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	7	55	with	mutants	765:771	arg1	activities					810:819	substantially improved specific activities	778:819	substantially improved specific activities	778:819	Three positive mutants with substantially improved specific activities were selected and studied.					
30797481	9	56	theme	%	1094:1094	arg1	improvement					1096:1106	80% improvement	1092:1106	80% improvement	1092:1106	Mutants Q112R and L211I also exhibited an apparently increased kcat/Km towards konjac glucomannan, approximately 200% and 80% improvement, respectively.					
30797481	6	57	from	mutagenesis	710:720	arg1	WB800					743:747	Bacillus subtilis WB800	725:747	Bacillus subtilis WB800	725:747	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	6	58	theme	high	583:586	arg1	activity					597:604	high specific activity	583:604	high specific activity	583:604	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	5	59	theme	sequence	329:336	arg1	identity					338:345	high sequence identity	324:345	high sequence identity	324:345	The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26.					
30797481	12	60	theme	β-mannanase	1478:1488	arg1	development					1443:1453	the development	1439:1453	the development of industrially useful β-mannanase	1439:1488	These new findings will help promote the development of industrially useful β-mannanase, with both good thermal stability and high specific activity.					
30797481	8	61	theme	amino	907:911	arg1	substitution					918:929	the single amino acid substitution	896:929	the single amino acid substitution	896:929	The best performing mutant was K291E, for which the single amino acid substitution led to a 3.5-fold increase in kcat/Km.					
30797481	7	62	theme	specific	801:808	arg1	activities					810:819	substantially improved specific activities	778:819	substantially improved specific activities	778:819	Three positive mutants with substantially improved specific activities were selected and studied.					
30797481	12	63	theme	specific	1533:1540	arg1	activity					1542:1549	high specific activity	1528:1549	high specific activity	1528:1549	These new findings will help promote the development of industrially useful β-mannanase, with both good thermal stability and high specific activity.					
30797481	6	64	theme	U/mg	501:504	arg1	activity					484:491	a specific activity	473:491	a specific activity of 2802 U/mg	473:504	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	12	65	theme	useful	1471:1476	arg1	β-mannanase					1478:1488	industrially useful β-mannanase	1458:1488	industrially useful β-mannanase	1458:1488	These new findings will help promote the development of industrially useful β-mannanase, with both good thermal stability and high specific activity.					
30797481	6	66	theme	Bman26	675:680	arg1	engineering					660:670	molecular engineering	650:670	molecular engineering of Bman26	650:680	The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800.					
30797481	0	67	theme	Bacillus	50:57	arg1	sp					59:60	Bacillus sp	50:60	Bacillus sp	50:60	Cloning and expression of a β-mannanase gene from Bacillus sp.					
30797481	12	68	theme	good	1501:1504	arg1	stability					1514:1522	good thermal stability	1501:1522	good thermal stability	1501:1522	These new findings will help promote the development of industrially useful β-mannanase, with both good thermal stability and high specific activity.					
30797481	9	69	theme	increased	1023:1031	arg1	kcat/Km					1033:1039	an apparently increased kcat/Km	1009:1039	an apparently increased kcat/Km	1009:1039	Mutants Q112R and L211I also exhibited an apparently increased kcat/Km towards konjac glucomannan, approximately 200% and 80% improvement, respectively.					
30797481	10	70	theme	Structural-functional	1123:1143	arg1	analysis					1145:1152	Structural-functional analysis	1123:1152	Structural-functional analysis	1123:1152	Structural-functional analysis indicated that a slight conformational change could dramatically affect certain enzyme characteristics.					
30337494	3	0	from	sensor	671:676	arg1	cells					705:709	human and murine immune cells	681:709	human and murine immune cells	681:709	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	1	theme	immune	698:703	arg1	cells					705:709	human and murine immune cells	681:709	human and murine immune cells	681:709	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	4	2	theme	mycobacterial	869:881	arg1	ligands					888:894	known mycobacterial TLR2 ligands	863:894	known mycobacterial TLR2 ligands	863:894	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	2	3	theme	polymeric	352:360	arg1	chitin					368:373	polymeric crude chitin	352:373	polymeric crude chitin with unknown purity and molecular composition	352:419	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	0	4	theme	dependent	71:79	arg1	inflammation					58:69	inflammation	58:69	inflammation dependent on oligomer size	58:96	The fungal ligand chitin directly binds TLR2 and triggers inflammation dependent on oligomer size.					
30337494	3	5	theme	human	681:685	arg1	cells					705:709	human and murine immune cells	681:709	human and murine immune cells	681:709	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	6	theme	fungal	657:662	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	6	theme	fungal	657:662	arg1	sensor					671:676	a primary fungal chitin sensor	647:676	a primary fungal chitin sensor on human and murine immune cells	647:709	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	6	7	theme	interaction	1111:1121	arg1	blocking					1083:1090	blocking	1083:1090	blocking of the chitin-TLR2 interaction	1083:1121	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	4	8	theme	TLR2	883:886	arg1	ligands					888:894	known mycobacterial TLR2 ligands	863:894	known mycobacterial TLR2 ligands	863:894	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	6	9	theme	fungal	1331:1336	arg1	disease					1338:1344	fungal disease	1331:1344	fungal disease	1331:1344	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	3	10	theme	chitin	453:458	arg1	oligomers					483:491	defined chitin (N-acetyl-glucosamine) oligomers	445:491	defined chitin (N-acetyl-glucosamine) oligomers	445:491	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	4	11	theme	TLR2	789:792	arg1	ligand					794:799	this fungal TLR2 ligand	777:799	this fungal TLR2 ligand	777:799	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	5	12	theme	immuno-modulation	1014:1030	arg1	system					1004:1009	a size-dependent system	987:1009	a size-dependent system of immuno-modulation that appears conserved in plants and humans	987:1074	Unexpectedly, chitin oligomers composed of five or less subunits are inactive, hinting to a size-dependent system of immuno-modulation that appears conserved in plants and humans.					
30337494	6	13	theme	potential	1249:1257	arg1	interaction					1232:1242	the chitin-TLR2 interaction	1216:1242	the chitin-TLR2 interaction	1216:1242	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	6	13	theme	potential	1249:1257	arg1	target					1259:1264	a potential target	1247:1264	a potential target for developing novel therapies in chitin-related pathologies and fungal disease	1247:1344	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	2	14	theme	immune	276:281	arg1	activation					283:292	signaling immune activation	266:292	signaling immune activation	266:292	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	0	15	theme	oligomer	84:91	arg1	size					93:96	oligomer size	84:96	oligomer size	84:96	The fungal ligand chitin directly binds TLR2 and triggers inflammation dependent on oligomer size.					
30337494	2	16	used	used	430:433	arg2	chitin					368:373	polymeric crude chitin	352:373	polymeric crude chitin with unknown purity and molecular composition	352:419	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	3	17	theme	smallest	549:556	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	17	theme	smallest	549:556	arg1	motif					581:585	the smallest immunologically active motif	545:585	the smallest immunologically active motif	545:585	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	6	18	theme	chitin-related	1300:1313	arg1	pathologies					1315:1325	chitin-related pathologies	1300:1325	chitin-related pathologies	1300:1325	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	2	19	theme	signaling	266:274	arg1	activation					283:292	signaling immune activation	266:292	signaling immune activation	266:292	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	4	20	theme	Chitin	712:717	arg1	oligomers					719:727	Chitin oligomers	712:727	Chitin oligomers	712:727	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	6	21	theme	chitin-TLR2	1099:1109	arg1	interaction					1111:1121	the chitin-TLR2 interaction	1095:1121	the chitin-TLR2 interaction	1095:1121	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	1	22	from	polysaccharide	134:147	arg1	nature					152:157	nature	152:157	nature	152:157	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	5	23	dep	appears	1037:1043	arg1	conserved					1045:1053	conserved	1045:1053	appears conserved in plants and humans	1037:1074	Unexpectedly, chitin oligomers composed of five or less subunits are inactive, hinting to a size-dependent system of immuno-modulation that appears conserved in plants and humans.					
30337494	6	24	from	therapies	1287:1295	arg1	pathologies					1315:1325	chitin-related pathologies	1300:1325	chitin-related pathologies	1300:1325	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	6	24	from	therapies	1287:1295	arg1	disease					1338:1344	fungal disease	1331:1344	fungal disease	1331:1344	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	5	25	theme	chitin	911:916	arg1	oligomers					918:926	chitin oligomers	911:926	chitin oligomers composed of five or less subunits	911:960	Unexpectedly, chitin oligomers composed of five or less subunits are inactive, hinting to a size-dependent system of immuno-modulation that appears conserved in plants and humans.					
30337494	4	26	theme	signaling	832:840	arg1	outcomes					842:849	overlapping and distinct signaling outcomes	807:849	overlapping and distinct signaling outcomes	807:849	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	0	27	theme	fungal	4:9	arg1	chitin					18:23	The fungal ligand chitin	0:23	The fungal ligand chitin	0:23	The fungal ligand chitin directly binds TLR2 and triggers inflammation dependent on oligomer size.					
30337494	6	28	theme	novel	1281:1285	arg1	therapies					1287:1295	novel therapies	1281:1295	novel therapies in chitin-related pathologies and fungal disease	1281:1344	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	2	29	theme	unknown	380:386	arg1	purity					388:393	unknown purity	380:393	unknown purity	380:393	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	1	30	theme	fungal	173:178	arg1	infection					180:188	fungal infection	173:188	fungal infection	173:188	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	6	31	theme	chitin-TLR2	1220:1230	arg1	interaction					1232:1242	the chitin-TLR2 interaction	1216:1242	the chitin-TLR2 interaction	1216:1242	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	6	31	theme	chitin-TLR2	1220:1230	arg1	target					1259:1264	a potential target	1247:1264	a potential target for developing novel therapies in chitin-related pathologies and fungal disease	1247:1344	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	4	32	theme	known	863:867	arg1	ligands					888:894	known mycobacterial TLR2 ligands	863:894	known mycobacterial TLR2 ligands	863:894	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	4	33	theme	overlapping	807:817	arg1	outcomes					842:849	overlapping and distinct signaling outcomes	807:849	overlapping and distinct signaling outcomes	807:849	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	0	34	theme	ligand	11:16	arg1	chitin					18:23	The fungal ligand chitin	0:23	The fungal ligand chitin	0:23	The fungal ligand chitin directly binds TLR2 and triggers inflammation dependent on oligomer size.					
30337494	1	35	attach	linked	163:168	arg2	Chitin					99:104	Chitin	99:104	Chitin	99:104	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	1	35	attach	linked	163:168	arg2	polysaccharide					134:147	the second most abundant polysaccharide	109:147	the second most abundant polysaccharide in nature	109:157	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	1	35	attach	linked	163:168	arg1	asthma					194:199	asthma	194:199	asthma	194:199	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	1	35	attach	linked	163:168	arg1	infection					180:188	fungal infection	173:188	fungal infection	173:188	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	3	36	theme	active	574:579	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	36	theme	active	574:579	arg1	motif					581:585	the smallest immunologically active motif	545:585	the smallest immunologically active motif	545:585	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	37	theme	six-subunit-long	511:526	arg1	sensor					671:676	a primary fungal chitin sensor	647:676	a primary fungal chitin sensor on human and murine immune cells	647:709	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	37	theme	six-subunit-long	511:526	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	37	theme	six-subunit-long	511:526	arg1	motif					581:585	the smallest immunologically active motif	545:585	the smallest immunologically active motif	545:585	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	37	theme	six-subunit-long	511:526	arg1	TLR2					638:641	the innate immune receptor Toll-like receptor (TLR2)	591:642	the innate immune receptor Toll-like receptor (TLR2)	591:642	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	38	theme	primary	649:655	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	38	theme	primary	649:655	arg1	sensor					671:676	a primary fungal chitin sensor	647:676	a primary fungal chitin sensor on human and murine immune cells	647:709	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	39	theme	immune	602:607	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	39	theme	immune	602:607	arg1	TLR2					638:641	the innate immune receptor Toll-like receptor (TLR2)	591:642	the innate immune receptor Toll-like receptor (TLR2)	591:642	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	40	theme	defined	445:451	arg1	oligomers					483:491	defined chitin (N-acetyl-glucosamine) oligomers	445:491	defined chitin (N-acetyl-glucosamine) oligomers	445:491	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	4	41	theme	nanomolar	753:761	arg1	affinity					763:770	nanomolar affinity	753:770	nanomolar affinity	753:770	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	2	42	theme	bona	211:214	arg1	receptors					228:236	bona fide immune receptors	211:236	bona fide immune receptors directly binding chitin and signaling immune activation and inflammation	211:309	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	2	43	theme	molecular	399:407	arg1	composition					409:419	molecular composition	399:419	molecular composition	399:419	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	4	44	with	TLR2	743:746	arg1	affinity					763:770	nanomolar affinity	753:770	nanomolar affinity	753:770	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	4	45	theme	distinct	823:830	arg1	outcomes					842:849	overlapping and distinct signaling outcomes	807:849	overlapping and distinct signaling outcomes	807:849	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	2	46	dep	bona	211:214	arg1	fide					216:219	fide	216:219	fide	216:219	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	3	47	theme	innate	595:600	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	47	theme	innate	595:600	arg1	TLR2					638:641	the innate immune receptor Toll-like receptor (TLR2)	591:642	the innate immune receptor Toll-like receptor (TLR2)	591:642	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	48	theme	receptor	628:635	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	48	theme	receptor	628:635	arg1	TLR2					638:641	the innate immune receptor Toll-like receptor (TLR2)	591:642	the innate immune receptor Toll-like receptor (TLR2)	591:642	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	4	49	theme	fungal	782:787	arg1	ligand					794:799	this fungal TLR2 ligand	777:799	this fungal TLR2 ligand	777:799	Chitin oligomers directly bind TLR2 with nanomolar affinity, and this fungal TLR2 ligand shows overlapping and distinct signaling outcomes compared to known mycobacterial TLR2 ligands.					
30337494	3	50	theme	receptor	609:616	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	50	theme	receptor	609:616	arg1	TLR2					638:641	the innate immune receptor Toll-like receptor (TLR2)	591:642	the innate immune receptor Toll-like receptor (TLR2)	591:642	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	1	51	theme	second	113:118	arg1	polysaccharide					134:147	the second most abundant polysaccharide	109:147	the second most abundant polysaccharide in nature	109:157	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	1	51	theme	second	113:118	arg1	Chitin					99:104	Chitin	99:104	Chitin	99:104	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	2	52	with	chitin	368:373	arg1	purity					388:393	unknown purity	380:393	unknown purity	380:393	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	2	52	with	chitin	368:373	arg1	composition					409:419	molecular composition	399:419	molecular composition	399:419	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	5	53	theme	size-dependent	989:1002	arg1	system					1004:1009	a size-dependent system	987:1009	a size-dependent system of immuno-modulation that appears conserved in plants and humans	987:1074	Unexpectedly, chitin oligomers composed of five or less subunits are inactive, hinting to a size-dependent system of immuno-modulation that appears conserved in plants and humans.					
30337494	2	54	theme	crude	362:366	arg1	chitin					368:373	polymeric crude chitin	352:373	polymeric crude chitin with unknown purity and molecular composition	352:419	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	6	55	theme	chitin-mediated	1144:1158	arg1	inflammation					1160:1171	chitin-mediated inflammation	1144:1171	chitin-mediated inflammation	1144:1171	Since blocking of the chitin-TLR2 interaction effectively prevents chitin-mediated inflammation in vitro and in vivo, our study highlights the chitin-TLR2 interaction as a potential target for developing novel therapies in chitin-related pathologies and fungal disease.					
30337494	3	56	theme	chitin	664:669	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	56	theme	chitin	664:669	arg1	sensor					671:676	a primary fungal chitin sensor	647:676	a primary fungal chitin sensor on human and murine immune cells	647:709	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	57	theme	Toll-like	618:626	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	57	theme	Toll-like	618:626	arg1	TLR2					638:641	the innate immune receptor Toll-like receptor (TLR2)	591:642	the innate immune receptor Toll-like receptor (TLR2)	591:642	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	1	58	theme	abundant	125:132	arg1	polysaccharide					134:147	the second most abundant polysaccharide	109:147	the second most abundant polysaccharide in nature	109:157	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	1	58	theme	abundant	125:132	arg1	Chitin					99:104	Chitin	99:104	Chitin	99:104	Chitin is the second most abundant polysaccharide in nature and linked to fungal infection and asthma.					
30337494	3	59	theme	murine	691:696	arg1	cells					705:709	human and murine immune cells	681:709	human and murine immune cells	681:709	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	2	60	theme	immune	221:226	arg1	receptors					228:236	bona fide immune receptors	211:236	bona fide immune receptors directly binding chitin and signaling immune activation and inflammation	211:309	However, bona fide immune receptors directly binding chitin and signaling immune activation and inflammation have not been clearly identified because polymeric crude chitin with unknown purity and molecular composition has been used.					
30337494	3	61	theme	chitin	528:533	arg1	sensor					671:676	a primary fungal chitin sensor	647:676	a primary fungal chitin sensor on human and murine immune cells	647:709	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	61	theme	chitin	528:533	arg1	chains					535:540	six-subunit-long chitin chains	511:540	six-subunit-long chitin chains	511:540	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	61	theme	chitin	528:533	arg1	motif					581:585	the smallest immunologically active motif	545:585	the smallest immunologically active motif	545:585	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337494	3	61	theme	chitin	528:533	arg1	TLR2					638:641	the innate immune receptor Toll-like receptor (TLR2)	591:642	the innate immune receptor Toll-like receptor (TLR2)	591:642	By using defined chitin (N-acetyl-glucosamine) oligomers, we here identify six-subunit-long chitin chains as the smallest immunologically active motif and the innate immune receptor Toll-like receptor (TLR2) as a primary fungal chitin sensor on human and murine immune cells.					
30337555	4	0	theme	lysis	911:915	arg1	buffer					917:922	The lysis buffer	907:922	The lysis buffer	907:922	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	5	1	theme	application	1036:1046	arg1	experiments					1048:1058	validation and application experiments	1021:1058	validation and application experiments	1021:1058	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	4	2	theme	calibration	818:828	arg1	mixture					739:745	A mixture	737:745	(2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix	733:800	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	2	theme	calibration	818:828	arg1	sample					839:844	the calibration standard sample	814:844	the calibration standard sample	814:844	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	1	3	from	present	177:183	arg1	tissues					195:201	animal tissues	188:201	animal tissues	188:201	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	1	4	theme	Gal	163:165	arg1	epitopes					168:175	Alpha-Gal (Gal) epitopes	152:175	Alpha-Gal (Gal) epitopes present in animal tissues	152:201	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	1	4	theme	Gal	163:165	arg1	xenoantigens					227:238	the key xenoantigens	219:238	the key xenoantigens that elicit xenorejection	219:264	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	0	5	theme	animal	78:83	arg1	tissues					85:91	animal tissues	78:91	animal tissues	78:91	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	2	6	link	tissue-derived	333:346	arg1	biomaterials					348:359	animal tissue-derived biomaterials	326:359	animal tissue-derived biomaterials	326:359	However, a standardized method to determine Gal epitope in animal tissue-derived biomaterials does not exist.					
30337555	3	7	theme	Gal	505:507	arg1	antibody					509:516	Gal antibody	505:516	Gal antibody	505:516	Herein, a standardized method for quantitative detection of Gal antigen was established based on an ELISA inhibition assay with Gal antibody.					
30337555	6	8	dep	Kit	1267:1269	arg1	Meitan					1272:1277	Meitan	1272:1277	Meitan	1272:1277	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	6	8	dep	Kit	1267:1269	arg1	China					1286:1290	China	1286:1290	China	1286:1290	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	0	9	theme	animal	96:101	arg1	biomaterials					118:129	animal tissue-derived biomaterials	96:129	animal tissue-derived biomaterials	96:129	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	2	10	from	epitope	315:321	arg1	biomaterials					348:359	animal tissue-derived biomaterials	326:359	animal tissue-derived biomaterials	326:359	However, a standardized method to determine Gal epitope in animal tissue-derived biomaterials does not exist.					
30337555	2	11	theme	standardized	278:289	arg1	method					291:296	a standardized method	276:296	a standardized method to determine Gal epitope in animal tissue-derived biomaterials	276:359	However, a standardized method to determine Gal epitope in animal tissue-derived biomaterials does not exist.					
30337555	6	12	theme	standard	1325:1332	arg1	it					1298:1299	it	1298:1299	it	1298:1299	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	6	12	theme	standard	1325:1332	arg1	method					1334:1339	a standard method	1323:1339	a standard method for detecting remnant immunogen of animal tissue derived medical devices	1323:1412	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	4	13	theme	standard	830:837	arg1	mixture					739:745	A mixture	737:745	(2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix	733:800	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	13	theme	standard	830:837	arg1	sample					839:844	the calibration standard sample	814:844	the calibration standard sample	814:844	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	3	14	theme	inhibition	483:492	arg1	assay					494:498	an ELISA inhibition assay	474:498	an ELISA inhibition assay with Gal antibody	474:516	Herein, a standardized method for quantitative detection of Gal antigen was established based on an ELISA inhibition assay with Gal antibody.					
30337555	6	15	link	derived	1390:1396	arg1	devices					1406:1412	animal tissue derived medical devices	1376:1412	animal tissue derived medical devices	1376:1412	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	4	16	contain	has	857:859	arg1	it					854:855	it	854:855	it	854:855	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	16	contain	has	857:859	arg2	composition					869:879	similar composition	861:879	similar composition with test sample	861:896	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	3	17	theme	Gal	437:439	arg1	antigen					441:447	Gal antigen	437:447	Gal antigen	437:447	Herein, a standardized method for quantitative detection of Gal antigen was established based on an ELISA inhibition assay with Gal antibody.					
30337555	0	18	theme	tissue-derived	103:116	arg1	biomaterials					118:129	animal tissue-derived biomaterials	96:129	animal tissue-derived biomaterials	96:129	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	4	19	theme	matrix	795:800	arg1	mixture					739:745	A mixture	737:745	(2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix	733:800	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	19	theme	matrix	795:800	arg1	sample					839:844	the calibration standard sample	814:844	the calibration standard sample	814:844	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	20	with	composition	869:879	arg1	sample					891:896	test sample	886:896	test sample	886:896	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	1	21	theme	present	177:183	arg1	epitopes					168:175	Alpha-Gal (Gal) epitopes	152:175	Alpha-Gal (Gal) epitopes present in animal tissues	152:201	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	1	21	theme	present	177:183	arg1	xenoantigens					227:238	the key xenoantigens	219:238	the key xenoantigens that elicit xenorejection	219:264	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	0	22	theme	standardized	2:13	arg1	method					28:33	A standardized quantitative method	0:33	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.	0:150	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	4	23	theme	optimized	543:551	arg1	conditions					566:575	the key optimized experimental conditions	535:575	the key optimized experimental conditions	535:575	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	2	24	theme	Gal	311:313	arg1	epitope					315:321	Gal epitope	311:321	Gal epitope in animal tissue-derived biomaterials	311:359	However, a standardized method to determine Gal epitope in animal tissue-derived biomaterials does not exist.					
30337555	4	25	dep	mixture	739:745	arg1	2					734:734	2	734:734	2	734:734	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	26	theme	key	539:541	arg1	conditions					566:575	the key optimized experimental conditions	535:575	the key optimized experimental conditions	535:575	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	3	27	with	assay	494:498	arg1	antibody					509:516	Gal antibody	505:516	Gal antibody	505:516	Herein, a standardized method for quantitative detection of Gal antigen was established based on an ELISA inhibition assay with Gal antibody.					
30337555	4	28	used	used	661:664	arg2	positive					599:606	positive	599:606	positive	599:606	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	28	used	used	661:664	arg2	control					691:697	positive and negative control	669:697	positive and negative control in the test system	669:716	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	29	theme	test	706:709	arg1	system					711:716	the test system	702:716	the test system	702:716	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	6	30	theme	devices	1406:1412	arg1	immunogen					1363:1371	remnant immunogen	1355:1371	remnant immunogen of animal tissue derived medical devices	1355:1412	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	1	31	theme	animal	188:193	arg1	tissues					195:201	animal tissues	188:201	animal tissues	188:201	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	5	32	theme	good	1100:1103	arg1	reproducibility					1105:1119	good reproducibility	1100:1119	good reproducibility (RSD = 12.48%)	1100:1134	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	5	32	theme	good	1100:1103	arg1	%					1133:1133	RSD = 12.48%	1122:1133	RSD = 12.48%	1122:1133	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	6	33	theme	detection	1257:1265	arg1	Kit					1267:1269	a detection Kit	1255:1269	a detection Kit (Meitan 70101, China)	1255:1291	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	6	34	theme	medical	1398:1404	arg1	devices					1406:1412	animal tissue derived medical devices	1376:1412	animal tissue derived medical devices	1376:1412	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	4	35	theme	negative	612:619	arg1	materials					631:639	(1) Gal-antigen positive and negative reference materials	583:639	materials	631:639	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	0	36	theme	quantitative	15:26	arg1	method					28:33	A standardized quantitative method	0:33	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.	0:150	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	4	37	theme	experimental	553:564	arg1	conditions					566:575	the key optimized experimental conditions	535:575	the key optimized experimental conditions	535:575	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	38	theme	similar	861:867	arg1	composition					869:879	similar composition	861:879	similar composition with test sample	861:896	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	1	39	attach	present	177:183	arg2	epitopes					168:175	Alpha-Gal (Gal) epitopes	152:175	Alpha-Gal (Gal) epitopes present in animal tissues	152:201	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	1	39	attach	present	177:183	arg2	xenoantigens					227:238	the key xenoantigens	219:238	the key xenoantigens that elicit xenorejection	219:264	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	1	39	attach	present	177:183	arg1	tissues					195:201	animal tissues	188:201	animal tissues	188:201	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	4	40	theme	positive	669:676	arg1	control					691:697	positive and negative control	669:697	positive and negative control in the test system	669:716	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	40	theme	positive	669:676	arg1	positive					599:606	positive	599:606	positive	599:606	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	6	41	theme	derived	1390:1396	arg1	devices					1406:1412	animal tissue derived medical devices	1376:1412	animal tissue derived medical devices	1376:1412	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	5	42	from	experiments	1048:1058	arg1	results					1008:1014	The results	1004:1014	The results from validation and application experiments	1004:1058	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	4	43	theme	negative	682:689	arg1	control					691:697	positive and negative control	669:697	positive and negative control in the test system	669:716	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	43	theme	negative	682:689	arg1	positive					599:606	positive	599:606	positive	599:606	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	44	theme	test	886:889	arg1	sample					891:896	test sample	886:896	test sample	886:896	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	45	theme	antigen	769:775	arg1	mixture					739:745	A mixture	737:745	(2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix	733:800	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	45	theme	antigen	769:775	arg1	sample					839:844	the calibration standard sample	814:844	the calibration standard sample	814:844	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	6	46	theme	tissue	1383:1388	arg1	devices					1406:1412	animal tissue derived medical devices	1376:1412	animal tissue derived medical devices	1376:1412	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	3	47	theme	quantitative	411:422	arg1	detection					424:432	quantitative detection	411:432	quantitative detection of Gal antigen	411:447	Herein, a standardized method for quantitative detection of Gal antigen was established based on an ELISA inhibition assay with Gal antibody.					
30337555	4	48	used	used	806:809	arg2	sample					839:844	the calibration standard sample	814:844	the calibration standard sample	814:844	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	48	used	used	806:809	arg2	mixture					739:745	A mixture	737:745	(2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix	733:800	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	5	49	contain	had	1096:1098	arg2	reproducibility					1105:1119	good reproducibility	1100:1119	good reproducibility (RSD = 12.48%)	1100:1134	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	5	49	contain	had	1096:1098	arg1	method					1089:1094	the standardized method	1072:1094	the standardized method	1072:1094	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	5	49	contain	had	1096:1098	arg2	%					1133:1133	RSD = 12.48%	1122:1133	RSD = 12.48%	1122:1133	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	4	50	dep	were	577:580	arg1	combined					928:935	combined	928:935	was combined with the homogenate to expose the Gal antigen as much as possible	924:1001	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	51	theme	Gal-BSA	761:767	arg1	antigen					769:775	artificial Gal-BSA antigen	750:775	artificial Gal-BSA antigen	750:775	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	4	52	theme	reference	621:629	arg1	materials					631:639	(1) Gal-antigen positive and negative reference materials	583:639	materials	631:639	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	0	53	theme	alpha-Gal	57:65	arg1	antigen					67:73	remnant alpha-Gal antigen	49:73	remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials	49:129	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	6	54	dep	developed	1310:1318	arg1	released					1453:1460	released	1453:1460	has been released in China	1444:1469	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	0	55	from	antigen	67:73	arg1	tissues					85:91	animal tissues	78:91	animal tissues	78:91	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	0	55	from	antigen	67:73	arg1	biomaterials					118:129	animal tissue-derived biomaterials	96:129	animal tissue-derived biomaterials	96:129	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	4	56	theme	Gal-negative	782:793	arg1	matrix					795:800	Gal-negative matrix	782:800	Gal-negative matrix	782:800	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	2	57	theme	tissue-derived	333:346	arg1	biomaterials					348:359	animal tissue-derived biomaterials	326:359	animal tissue-derived biomaterials	326:359	However, a standardized method to determine Gal epitope in animal tissue-derived biomaterials does not exist.					
30337555	0	58	theme	remnant	49:55	arg1	antigen					67:73	remnant alpha-Gal antigen	49:73	remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials	49:129	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	5	59	theme	standardized	1076:1087	arg1	method					1089:1094	the standardized method	1072:1094	the standardized method	1072:1094	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	5	60	theme	validation	1021:1030	arg1	experiments					1048:1058	validation and application experiments	1021:1058	validation and application experiments	1021:1058	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	4	61	dep	positive	599:606	arg1	1					584:584	1	584:584	1	584:584	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	6	62	theme	remnant	1355:1361	arg1	immunogen					1363:1371	remnant immunogen	1355:1371	remnant immunogen of animal tissue derived medical devices	1355:1412	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	1	63	from	tissues	195:201	arg1	present					177:183	present	177:183	present	177:183	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	2	64	theme	animal	326:331	arg1	biomaterials					348:359	animal tissue-derived biomaterials	326:359	animal tissue-derived biomaterials	326:359	However, a standardized method to determine Gal epitope in animal tissue-derived biomaterials does not exist.					
30337555	6	65	theme	animal	1376:1381	arg1	devices					1406:1412	animal tissue derived medical devices	1376:1412	animal tissue derived medical devices	1376:1412	This method has been further developed into a detection Kit (Meitan 70101, China), and it has been developed as a standard method for detecting remnant immunogen of animal tissue derived medical devices, and as the industry standard has been released in China.					
30337555	5	66	theme	~7.1 × 1011 Gal	1176:1190	arg1	limit					1161:1165	the lower detection limit	1141:1165	the lower detection limit (LDL)	1141:1171	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	5	66	theme	~7.1 × 1011 Gal	1176:1190	arg1	epitopes/reaction					1192:1208	~7.1 × 1011 Gal epitopes/reaction	1176:1208	~7.1 × 1011 Gal epitopes/reaction	1176:1208	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	3	67	theme	ELISA	477:481	arg1	assay					494:498	an ELISA inhibition assay	474:498	an ELISA inhibition assay with Gal antibody	474:516	Herein, a standardized method for quantitative detection of Gal antigen was established based on an ELISA inhibition assay with Gal antibody.					
30337555	4	68	from	control	691:697	arg1	system					711:716	the test system	702:716	the test system	702:716	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	0	69	from	application	139:149	arg1	tissues					85:91	animal tissues	78:91	animal tissues	78:91	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	0	69	from	application	139:149	arg1	biomaterials					118:129	animal tissue-derived biomaterials	96:129	animal tissue-derived biomaterials	96:129	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	4	70	theme	artificial	750:759	arg1	antigen					769:775	artificial Gal-BSA antigen	750:775	artificial Gal-BSA antigen	750:775	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	0	71	link	tissue-derived	103:116	arg1	biomaterials					118:129	animal tissue-derived biomaterials	96:129	animal tissue-derived biomaterials	96:129	A standardized quantitative method for detecting remnant alpha-Gal antigen in animal tissues or animal tissue-derived biomaterials and its application.					
30337555	1	72	theme	Alpha-Gal	152:160	arg1	epitopes					168:175	Alpha-Gal (Gal) epitopes	152:175	Alpha-Gal (Gal) epitopes present in animal tissues	152:201	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	1	72	theme	Alpha-Gal	152:160	arg1	xenoantigens					227:238	the key xenoantigens	219:238	the key xenoantigens that elicit xenorejection	219:264	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	3	73	theme	antigen	441:447	arg1	detection					424:432	quantitative detection	411:432	quantitative detection of Gal antigen	411:447	Herein, a standardized method for quantitative detection of Gal antigen was established based on an ELISA inhibition assay with Gal antibody.					
30337555	1	74	theme	key	223:225	arg1	epitopes					168:175	Alpha-Gal (Gal) epitopes	152:175	Alpha-Gal (Gal) epitopes present in animal tissues	152:201	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	1	74	theme	key	223:225	arg1	xenoantigens					227:238	the key xenoantigens	219:238	the key xenoantigens that elicit xenorejection	219:264	Alpha-Gal (Gal) epitopes present in animal tissues are known to be the key xenoantigens that elicit xenorejection.					
30337555	3	75	theme	standardized	387:398	arg1	method					400:405	a standardized method	385:405	a standardized method for quantitative detection of Gal antigen	385:447	Herein, a standardized method for quantitative detection of Gal antigen was established based on an ELISA inhibition assay with Gal antibody.					
30337555	5	76	theme	lower	1145:1149	arg1	LDL					1168:1170	LDL	1168:1170	LDL	1168:1170	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	5	76	theme	lower	1145:1149	arg1	limit					1161:1165	the lower detection limit	1141:1165	the lower detection limit (LDL)	1141:1171	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	5	76	theme	lower	1145:1149	arg1	epitopes/reaction					1192:1208	~7.1 × 1011 Gal epitopes/reaction	1176:1208	~7.1 × 1011 Gal epitopes/reaction	1176:1208	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	4	77	theme	Gal	971:973	arg1	antigen					975:981	the Gal antigen	967:981	the Gal antigen	967:981	In this method, the key optimized experimental conditions were: (1) Gal-antigen positive and negative reference materials were developed, and used as positive and negative control in the test system, respectively; (2) A mixture of artificial Gal-BSA antigen plus Gal-negative matrix was used as the calibration standard sample, making it has similar composition with test sample; and (3) The lysis buffer was combined with the homogenate to expose the Gal antigen as much as possible.					
30337555	5	78	theme	detection	1151:1159	arg1	LDL					1168:1170	LDL	1168:1170	LDL	1168:1170	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	5	78	theme	detection	1151:1159	arg1	limit					1161:1165	the lower detection limit	1141:1165	the lower detection limit (LDL)	1141:1171	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
30337555	5	78	theme	detection	1151:1159	arg1	epitopes/reaction					1192:1208	~7.1 × 1011 Gal epitopes/reaction	1176:1208	~7.1 × 1011 Gal epitopes/reaction	1176:1208	The results from validation and application experiments showed that the standardized method had good reproducibility (RSD = 12.48%), and the lower detection limit (LDL) is ~7.1 × 1011 Gal epitopes/reaction.					
31340177	4	0	theme	C	863:863	arg1	142.19 kDa					870:879	142.19 kDa	870:879	142.19 kDa	870:879	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	0	theme	C	863:863	arg1	24h					865:867	C 24h	863:867	C 24h	863:867	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	1	theme	CDP	806:808	arg1	40 °C					773:777	40 °C	773:777	40 °C	773:777	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	5	2	theme	CDP	1004:1006	arg1	antioxidant					960:970	antioxidant	960:970	antioxidant	960:970	Following their physicochemical characterization, the functional properties, antioxidant and antimicrobial activities of CDP were investigated.					
31340177	5	2	theme	CDP	1004:1006	arg1	activities					990:999	antimicrobial activities	976:999	antimicrobial activities	976:999	Following their physicochemical characterization, the functional properties, antioxidant and antimicrobial activities of CDP were investigated.					
31340177	5	2	theme	CDP	1004:1006	arg1	properties					948:957	the functional properties	933:957	the functional properties	933:957	Following their physicochemical characterization, the functional properties, antioxidant and antimicrobial activities of CDP were investigated.					
31340177	0	3	from	shells	161:166	arg1	application					73:83	its application	69:83	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells	0:166	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	0	3	from	shells	161:166	arg1	Preparation					0:10	Preparation	0:10	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells	0:166	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	6	4	theme	tested	1244:1249	arg1	bacteria					1251:1258	all tested bacteria	1240:1258	all tested bacteria	1240:1258	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	3	5	theme	MALDI-TOF	648:656	arg1	MS					658:659	MALDI-TOF MS	648:659	MALDI-TOF MS owing to an endo-cleavage mode	648:690	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	4	6	theme	water	792:796	arg1	CDP					806:808	a low Mw water soluble CDP	783:808	a low Mw water soluble CDP (H 24h, <4.4 kDa)	783:826	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	2	7	theme	optimum	329:335	arg1	activity					337:344	optimum activity	329:344	optimum activity	329:344	The crude chitosanase showed optimum activity at pH 4.0 and 60 °C and retained >80% of its activity over a pH range from 3.0 to 10.0.					
31340177	1	8	theme	Portunus	233:240	arg1	crab					227:230	blue crab	222:230	blue crab (Portunus segnis) viscera	222:256	In this study, a digestive chitosanase from blue crab (Portunus segnis) viscera was extracted, characterized and applied.					
31340177	1	8	theme	Portunus	233:240	arg1	segnis					242:247	Portunus segnis	233:247	Portunus segnis	233:247	In this study, a digestive chitosanase from blue crab (Portunus segnis) viscera was extracted, characterized and applied.					
31340177	0	9	from	Preparation	0:10	arg1	production					92:101	the production	88:101	the production of biologically active chito-oligosaccharides	88:147	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	0	9	from	Preparation	0:10	arg1	viscera					50:56	blue crab viscera	40:56	blue crab viscera	40:56	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	4	10	theme	H	811:811	arg1	<4.4 kDa					818:825	<4.4 kDa	818:825	<4.4 kDa	818:825	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	10	theme	H	811:811	arg1	24h					813:815	H 24h	811:815	H 24h	811:815	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	7	11	theme	simple	1420:1425	arg1	method					1451:1456	a simple, inexpensive and easily method	1418:1456	a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1418:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	7	11	theme	simple	1420:1425	arg1	chitosanase					1403:1413	the Portunus segnis digestive crude chitosanase	1367:1413	the Portunus segnis digestive crude chitosanase	1367:1413	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	4	12	theme	hydrolysis	698:707	arg1	process					709:715	This hydrolysis process	693:715	This hydrolysis process	693:715	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	5	13	theme	antimicrobial	976:988	arg1	activities					990:999	antimicrobial activities	976:999	antimicrobial activities	976:999	Following their physicochemical characterization, the functional properties, antioxidant and antimicrobial activities of CDP were investigated.					
31340177	6	14	theme	antioxidant	1138:1148	arg1	activities					1150:1159	potent antioxidant activities	1131:1159	potent antioxidant activities	1131:1159	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	3	15	theme	bioactive	493:501	arg1	CDP					592:594	CDP	592:594	CDP	592:594	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	3	15	theme	bioactive	493:501	arg1	products					582:589	bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products	493:589	bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP)	493:595	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	6	16	theme	high	1168:1171	arg1	derivatives					1176:1186	high Mw derivatives	1168:1186	high Mw derivatives	1168:1186	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	6	16	theme	high	1168:1171	arg1	24h					1202:1204	C 24h	1200:1204	especially C 24h	1189:1204	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	4	17	theme	high	850:853	arg1	CDP					858:860	a high Mw CDP	848:860	a high Mw CDP (C 24h, 142.19 kDa)	848:880	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	18	theme	low	785:787	arg1	CDP					806:808	a low Mw water soluble CDP	783:808	a low Mw water soluble CDP (H 24h, <4.4 kDa)	783:826	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	6	19	theme	native	1061:1066	arg1	CDP					1078:1080	CDP	1078:1080	CDP	1078:1080	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	6	19	theme	native	1061:1066	arg1	chitosan					1068:1075	the native chitosan	1057:1075	the native chitosan	1057:1075	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	4	20	dep	CDP	806:808	arg1	<4.4 kDa					818:825	<4.4 kDa	818:825	<4.4 kDa	818:825	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	20	dep	CDP	806:808	arg1	24h					813:815	H 24h	811:815	H 24h	811:815	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	3	21	dep	sequences	612:620	arg1	determined					634:643	determined	634:643	determined by MALDI-TOF MS owing to an endo-cleavage mode	634:690	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	7	22	theme	inexpensive	1428:1438	arg1	method					1451:1456	a simple, inexpensive and easily method	1418:1456	a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1418:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	7	22	theme	inexpensive	1428:1438	arg1	chitosanase					1403:1413	the Portunus segnis digestive crude chitosanase	1367:1413	the Portunus segnis digestive crude chitosanase	1367:1413	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	7	23	theme	Portunus	1371:1378	arg1	chitosanase					1403:1413	the Portunus segnis digestive crude chitosanase	1367:1413	the Portunus segnis digestive crude chitosanase	1367:1413	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	7	23	theme	Portunus	1371:1378	arg1	method					1451:1456	a simple, inexpensive and easily method	1418:1456	a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1418:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	7	24	theme	bioactive	1469:1477	arg1	chitosan-derivatives					1479:1498	bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1469:1575	bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1469:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	2	25	theme	pH	407:408	arg1	range					410:414	a pH range	405:414	a pH range from 3.0 to 10.0	405:431	The crude chitosanase showed optimum activity at pH 4.0 and 60 °C and retained >80% of its activity over a pH range from 3.0 to 10.0.					
31340177	3	26	theme	endo-cleavage	673:685	arg1	mode					687:690	an endo-cleavage mode	670:690	an endo-cleavage mode	670:690	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	1	27	from	viscera	250:256	arg1	chitosanase					205:215	a digestive chitosanase	193:215	a digestive chitosanase from blue crab (Portunus segnis) viscera	193:256	In this study, a digestive chitosanase from blue crab (Portunus segnis) viscera was extracted, characterized and applied.					
31340177	7	28	dep	Portunus	1371:1378	arg1	segnis					1380:1385	segnis	1380:1385	segnis	1380:1385	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	3	29	theme	weight	521:526	arg1	CDP					592:594	CDP	592:594	CDP	592:594	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	3	29	theme	weight	521:526	arg1	products					582:589	bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products	493:589	bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP)	493:595	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	2	30	dep	10.0	428:431	arg1	to					425:426	to	425:426	to	425:426	The crude chitosanase showed optimum activity at pH 4.0 and 60 °C and retained >80% of its activity over a pH range from 3.0 to 10.0.					
31340177	6	31	theme	C	1200:1200	arg1	derivatives					1176:1186	high Mw derivatives	1168:1186	high Mw derivatives	1168:1186	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	6	31	theme	C	1200:1200	arg1	24h					1202:1204	C 24h	1200:1204	especially C 24h	1189:1204	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	4	32	theme	Mw	789:790	arg1	CDP					806:808	a low Mw water soluble CDP	783:808	a low Mw water soluble CDP (H 24h, <4.4 kDa)	783:826	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	6	33	theme	potent	1131:1136	arg1	activities					1150:1159	potent antioxidant activities	1131:1159	potent antioxidant activities	1131:1159	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	7	34	theme	attractive	1550:1559	arg1	characteristics					1561:1575	their attractive characteristics	1544:1575	their attractive characteristics	1544:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	4	35	theme	soluble	798:804	arg1	CDP					806:808	a low Mw water soluble CDP	783:808	a low Mw water soluble CDP (H 24h, <4.4 kDa)	783:826	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	0	36	theme	crude	17:21	arg1	chitosanase					23:33	a crude chitosanase	15:33	a crude chitosanase from blue crab viscera	15:56	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	4	37	theme	incubation	759:768	arg1	CDP					858:860	a high Mw CDP	848:860	a high Mw CDP (C 24h, 142.19 kDa)	848:880	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	37	theme	incubation	759:768	arg1	24 h					751:754	24 h	751:754	24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6	751:842	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	6	38	theme	Mw	1098:1099	arg1	derivatives					1101:1111	especially low Mw derivatives	1083:1111	especially low Mw derivatives (H 24h)	1083:1119	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	6	38	theme	Mw	1098:1099	arg1	24h					1116:1118	H 24h	1114:1118	H 24h	1114:1118	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	3	39	theme	varying	503:509	arg1	Mw					529:530	Mw	529:530	Mw	529:530	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	3	39	theme	varying	503:509	arg1	weight					521:526	varying molecular weight	503:526	varying molecular weight (Mw)	503:531	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	2	40	theme	activity	391:398	arg1	%					382:382	>80%	379:382	>80% of its activity	379:398	The crude chitosanase showed optimum activity at pH 4.0 and 60 °C and retained >80% of its activity over a pH range from 3.0 to 10.0.					
31340177	2	40	theme	activity	391:398	arg1	activity					391:398	its activity	387:398	its activity	387:398	The crude chitosanase showed optimum activity at pH 4.0 and 60 °C and retained >80% of its activity over a pH range from 3.0 to 10.0.					
31340177	4	41	from	40 °C	773:777	arg1	CDP					858:860	a high Mw CDP	848:860	a high Mw CDP (C 24h, 142.19 kDa)	848:880	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	41	from	40 °C	773:777	arg1	incubation					759:768	incubation	759:768	incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6	759:842	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	41	from	40 °C	773:777	arg1	24 h					751:754	24 h	751:754	24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6	751:842	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	3	42	theme	molecular	511:519	arg1	Mw					529:530	Mw	529:530	Mw	529:530	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	3	42	theme	molecular	511:519	arg1	weight					521:526	varying molecular weight	503:526	varying molecular weight (Mw)	503:531	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	0	43	theme	active	119:124	arg1	chito-oligosaccharides					126:147	biologically active chito-oligosaccharides	106:147	biologically active chito-oligosaccharides	106:147	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	2	44	from	10.0	428:431	arg1	range					410:414	a pH range	405:414	a pH range from 3.0 to 10.0	405:431	The crude chitosanase showed optimum activity at pH 4.0 and 60 °C and retained >80% of its activity over a pH range from 3.0 to 10.0.					
31340177	6	45	theme	H	1114:1114	arg1	derivatives					1101:1111	especially low Mw derivatives	1083:1111	especially low Mw derivatives (H 24h)	1083:1119	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	6	45	theme	H	1114:1114	arg1	24h					1116:1118	H 24h	1114:1118	H 24h	1114:1118	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	7	46	theme	novel	1297:1301	arg1	insights					1303:1310	novel insights	1297:1310	novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1297:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	0	47	theme	chitosanase	23:33	arg1	application					73:83	its application	69:83	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells	0:166	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	0	47	theme	chitosanase	23:33	arg1	Preparation					0:10	Preparation	0:10	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells	0:166	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	4	48	dep	6	842:842	arg1	to					839:840	to	839:840	to	839:840	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	7	49	theme	crude	1397:1401	arg1	chitosanase					1403:1413	the Portunus segnis digestive crude chitosanase	1367:1413	the Portunus segnis digestive crude chitosanase	1367:1413	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	7	49	theme	crude	1397:1401	arg1	method					1451:1456	a simple, inexpensive and easily method	1418:1456	a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1418:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	3	50	theme	acetylation	537:547	arg1	chitosan-depolymerization					556:580	acetylation degree chitosan-depolymerization	537:580	acetylation degree chitosan-depolymerization	537:580	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	1	51	theme	digestive	195:203	arg1	chitosanase					205:215	a digestive chitosanase	193:215	a digestive chitosanase from blue crab (Portunus segnis) viscera	193:256	In this study, a digestive chitosanase from blue crab (Portunus segnis) viscera was extracted, characterized and applied.					
31340177	0	52	theme	crab	45:48	arg1	viscera					50:56	blue crab viscera	40:56	blue crab viscera	40:56	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	0	53	from	viscera	50:56	arg1	application					73:83	its application	69:83	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells	0:166	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	0	53	from	viscera	50:56	arg1	Preparation					0:10	Preparation	0:10	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells	0:166	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	0	53	from	viscera	50:56	arg1	chitosanase					23:33	a crude chitosanase	15:33	a crude chitosanase from blue crab viscera	15:56	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	6	54	theme	Mw	1173:1174	arg1	derivatives					1176:1186	high Mw derivatives	1168:1186	high Mw derivatives	1168:1186	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	6	54	theme	Mw	1173:1174	arg1	24h					1202:1204	C 24h	1200:1204	especially C 24h	1189:1204	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	0	55	theme	blue	40:43	arg1	viscera					50:56	blue crab viscera	40:56	blue crab viscera	40:56	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	6	56	theme	fungi	1264:1268	arg1	growth					1230:1235	the growth	1226:1235	the growth of all tested bacteria and fungi	1226:1268	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	0	57	theme	chito-oligosaccharides	126:147	arg1	production					92:101	the production	88:101	the production of biologically active chito-oligosaccharides	88:147	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	5	58	theme	physicochemical	899:913	arg1	characterization					915:930	their physicochemical characterization	893:930	their physicochemical characterization	893:930	Following their physicochemical characterization, the functional properties, antioxidant and antimicrobial activities of CDP were investigated.					
31340177	3	59	theme	crude	452:456	arg1	chitosanase					458:468	the crude chitosanase	448:468	the crude chitosanase	448:468	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	7	60	theme	chitosan	1335:1342	arg1	depolymerisation					1344:1359	chitosan depolymerisation	1335:1359	chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1335:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	5	61	theme	functional	937:946	arg1	properties					948:957	the functional properties	933:957	the functional properties	933:957	Following their physicochemical characterization, the functional properties, antioxidant and antimicrobial activities of CDP were investigated.					
31340177	4	62	dep	CDP	858:860	arg1	142.19 kDa					870:879	142.19 kDa	870:879	142.19 kDa	870:879	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	62	dep	CDP	858:860	arg1	24h					865:867	C 24h	863:867	C 24h	863:867	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	7	63	theme	digestive	1387:1395	arg1	chitosanase					1403:1413	the Portunus segnis digestive crude chitosanase	1367:1413	the Portunus segnis digestive crude chitosanase	1367:1413	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	7	63	theme	digestive	1387:1395	arg1	method					1451:1456	a simple, inexpensive and easily method	1418:1456	a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1418:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	6	64	theme	low	1094:1096	arg1	derivatives					1101:1111	especially low Mw derivatives	1083:1111	especially low Mw derivatives (H 24h)	1083:1119	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	6	64	theme	low	1094:1096	arg1	24h					1116:1118	H 24h	1114:1118	H 24h	1114:1118	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	2	65	theme	crude	304:308	arg1	chitosanase					310:320	The crude chitosanase	300:320	The crude chitosanase	300:320	The crude chitosanase showed optimum activity at pH 4.0 and 60 °C and retained >80% of its activity over a pH range from 3.0 to 10.0.					
31340177	0	66	theme	shrimp	154:159	arg1	shells					161:166	shrimp shells	154:166	shrimp shells	154:166	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	0	67	from	application	73:83	arg1	production					92:101	the production	88:101	the production of biologically active chito-oligosaccharides	88:147	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	0	67	from	application	73:83	arg1	viscera					50:56	blue crab viscera	40:56	blue crab viscera	40:56	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	6	68	theme	bacteria	1251:1258	arg1	growth					1230:1235	the growth	1226:1235	the growth of all tested bacteria and fungi	1226:1268	Interestingly, as compared to the native chitosan, CDP, especially low Mw derivatives (H 24h) exhibited potent antioxidant activities, while high Mw derivatives, especially C 24h, markedly inhibited the growth of all tested bacteria and fungi.					
31340177	0	69	dep	chitosan	168:175	arg1	application					73:83	its application	69:83	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells	0:166	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	0	69	dep	chitosan	168:175	arg1	Preparation					0:10	Preparation	0:10	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells	0:166	Preparation of a crude chitosanase from blue crab viscera as well as its application in the production of biologically active chito-oligosaccharides from shrimp shells chitosan.					
31340177	3	70	theme	degree	549:554	arg1	chitosan-depolymerization					556:580	acetylation degree chitosan-depolymerization	537:580	acetylation degree chitosan-depolymerization	537:580	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	1	71	theme	blue	222:225	arg1	crab					227:230	blue crab	222:230	blue crab (Portunus segnis) viscera	222:256	In this study, a digestive chitosanase from blue crab (Portunus segnis) viscera was extracted, characterized and applied.					
31340177	1	71	theme	blue	222:225	arg1	segnis					242:247	Portunus segnis	233:247	Portunus segnis	233:247	In this study, a digestive chitosanase from blue crab (Portunus segnis) viscera was extracted, characterized and applied.					
31340177	4	72	theme	Mw	855:856	arg1	CDP					858:860	a high Mw CDP	848:860	a high Mw CDP (C 24h, 142.19 kDa)	848:880	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	7	73	theme	depolymerisation	1344:1359	arg1	efficiency					1321:1330	the efficiency	1317:1330	the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics	1317:1575	These results may provide novel insights into the efficiency of chitosan depolymerisation using the Portunus segnis digestive crude chitosanase as a simple, inexpensive and easily method to produce bioactive chitosan-derivatives and that this bioactivity depends highly on their attractive characteristics.					
31340177	3	74	theme	chitosan-depolymerization	556:580	arg1	CDP					592:594	CDP	592:594	CDP	592:594	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	3	74	theme	chitosan-depolymerization	556:580	arg1	products					582:589	bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products	493:589	bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP)	493:595	Subsequently, the crude chitosanase was applied to produce bioactive varying molecular weight (Mw) and acetylation degree chitosan-depolymerization products (CDP) with specially sequences composition determined by MALDI-TOF MS owing to an endo-cleavage mode.					
31340177	4	75	from	24 h	751:754	arg1	40 °C					773:777	40 °C	773:777	40 °C	773:777	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	76	from	CDP	858:860	arg1	40 °C					773:777	40 °C	773:777	40 °C	773:777	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	4	77	with	incubation	759:768	arg1	DP					833:834	DP	833:834	DP up to 6	833:842	This hydrolysis process allowed to the preparation, after 24 h of incubation at 40 °C, of a low Mw water soluble CDP (H 24h, <4.4 kDa) with DP up to 6 and a high Mw CDP (C 24h, 142.19 kDa).					
31340177	1	78	theme	crab	227:230	arg1	viscera					250:256	blue crab (Portunus segnis) viscera	222:256	blue crab (Portunus segnis) viscera	222:256	In this study, a digestive chitosanase from blue crab (Portunus segnis) viscera was extracted, characterized and applied.					
30361005	0	0	theme	varieties	89:97	arg1	composition					22:32	composition	22:32	composition	22:32	Relationships between composition, microstructure and cooking performances of six potato varieties.					
30361005	0	0	theme	varieties	89:97	arg1	microstructure					35:48	microstructure	35:48	microstructure	35:48	Relationships between composition, microstructure and cooking performances of six potato varieties.					
30361005	0	0	theme	varieties	89:97	arg1	performances					62:73	cooking performances	54:73	cooking performances	54:73	Relationships between composition, microstructure and cooking performances of six potato varieties.					
30361005	7	1	theme	Young	1163:1167	arg1	modulus					1171:1177	Young's modulus	1163:1177	Young's modulus	1163:1177	Fried Agria and Spunta, (56.3 and 61 kPa, respectively) had the smallest value of Young's modulus.					
30361005	4	2	theme	transition	677:686	arg1	dependent					814:822	dependent	814:822	dependent	814:822	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	4	2	theme	transition	677:686	arg1	temperatures					688:699	the transition temperatures	673:699	the transition temperatures (ranging between 60 °C and 85 °C)	673:733	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	8	3	theme	diagnostic	1289:1298	arg1	markers					1300:1306	diagnostic markers	1289:1306	diagnostic markers	1289:1306	Molecular marker analyses provided a genetic fingerprinting of our varieties, allowing the identification of diagnostic markers.					
30361005	1	4	theme	food	156:159	arg1	materials					128:136	the raw materials	120:136	the raw materials	120:136	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	1	4	theme	food	156:159	arg1	potatoes					177:184	cooked potatoes	170:184	cooked potatoes in hot water, baked potatoes and the most popular fried potatoes	170:249	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	1	4	theme	food	156:159	arg1	tubers					109:114	Potatoes tubers	100:114	Potatoes tubers	100:114	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	6	5	theme	baked	985:989	arg1	Lady					991:994	baked Lady Rosetta	985:1002	baked Lady Rosetta (224.3 kPa)	985:1014	In particular, baked Lady Rosetta (224.3 kPa) showed the least decrease in rigidity between thermal processes.					
30361005	6	5	theme	baked	985:989	arg1	224.3 kPa					1005:1013	224.3 kPa	1005:1013	224.3 kPa	1005:1013	In particular, baked Lady Rosetta (224.3 kPa) showed the least decrease in rigidity between thermal processes.					
30361005	0	6	theme	potato	82:87	arg1	varieties					89:97	six potato varieties	78:97	six potato varieties	78:97	Relationships between composition, microstructure and cooking performances of six potato varieties.					
30361005	11	7	theme	food	1617:1620	arg1	traits					1636:1641	appreciable food technological traits	1605:1641	appreciable food technological traits	1605:1641	The information obtained in this work may be useful to further study and associate genetic sequences with appreciable food technological traits.					
30361005	10	8	theme	exclusive	1418:1426	arg1	traits					1439:1444	its exclusive phenotypic traits	1414:1444	its exclusive phenotypic traits	1414:1444	Such distance corresponded to its exclusive phenotypic traits, that are known to affect thermochemical properties.					
30361005	5	9	theme	samples	871:877	arg1	modulus					853:859	the elasticity modulus	838:859	the elasticity modulus of cooked samples	838:877	In addition, the elasticity modulus of cooked samples depended on process type and followed the order: baked potatoes > boiled > fried potatoes.					
30361005	2	10	theme	boiling	306:312	arg1	impact					296:301	the impact	292:301	the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin	292:469	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	1	11	theme	popular	228:234	arg1	potatoes					242:249	the most popular fried potatoes	219:249	the most popular fried potatoes	219:249	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	11	12	theme	technological	1622:1634	arg1	traits					1636:1641	appreciable food technological traits	1605:1641	appreciable food technological traits	1605:1641	The information obtained in this work may be useful to further study and associate genetic sequences with appreciable food technological traits.					
30361005	6	13	from	decrease	1033:1040	arg1	rigidity					1045:1052	rigidity	1045:1052	rigidity between thermal processes	1045:1078	In particular, baked Lady Rosetta (224.3 kPa) showed the least decrease in rigidity between thermal processes.					
30361005	5	14	theme	baked	928:932	arg1	potatoes					960:967	baked potatoes > boiled > fried potatoes	928:967	baked potatoes > boiled > fried potatoes	928:967	In addition, the elasticity modulus of cooked samples depended on process type and followed the order: baked potatoes > boiled > fried potatoes.					
30361005	1	15	theme	fried	236:240	arg1	potatoes					242:249	the most popular fried potatoes	219:249	the most popular fried potatoes	219:249	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	5	16	theme	potatoes > boiled > fried	934:958	arg1	potatoes					960:967	baked potatoes > boiled > fried potatoes	928:967	baked potatoes > boiled > fried potatoes	928:967	In addition, the elasticity modulus of cooked samples depended on process type and followed the order: baked potatoes > boiled > fried potatoes.					
30361005	3	17	theme	Scanning	472:479	arg1	Microscopy					490:499	Scanning Electron Microscopy	472:499	Scanning Electron Microscopy	472:499	Scanning Electron Microscopy revealed significant differences between varieties and tuber microstructure changes following all cooking processes.					
30361005	8	18	theme	genetic	1217:1223	arg1	fingerprinting					1225:1238	a genetic fingerprinting	1215:1238	a genetic fingerprinting of our varieties, allowing the identification of diagnostic markers	1215:1306	Molecular marker analyses provided a genetic fingerprinting of our varieties, allowing the identification of diagnostic markers.					
30361005	6	19	dep	Lady	991:994	arg1	Rosetta					996:1002	Rosetta	996:1002	Rosetta	996:1002	In particular, baked Lady Rosetta (224.3 kPa) showed the least decrease in rigidity between thermal processes.					
30361005	2	20	from	impact	296:301	arg1	microstructure					336:349	microstructure	336:349	microstructure	336:349	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	2	20	from	impact	296:301	arg1	properties					355:364	properties	355:364	properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin	355:469	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	4	21	theme	gelatinization	753:766	arg1	enthalpies					739:748	enthalpies	739:748	enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers	739:794	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	4	21	theme	gelatinization	753:766	arg1	dependent					814:822	dependent	814:822	dependent	814:822	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	4	21	theme	gelatinization	753:766	arg1	temperatures					688:699	the transition temperatures	673:699	the transition temperatures (ranging between 60 °C and 85 °C)	673:733	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	5	22	theme	elasticity	842:851	arg1	modulus					853:859	the elasticity modulus	838:859	the elasticity modulus of cooked samples	838:877	In addition, the elasticity modulus of cooked samples depended on process type and followed the order: baked potatoes > boiled > fried potatoes.					
30361005	4	23	theme	Scanning	631:638	arg1	analysis					652:659	Differential Scanning Calorimeter analysis	618:659	Differential Scanning Calorimeter analysis	618:659	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	7	24	theme	smallest	1145:1152	arg1	value					1154:1158	the smallest value	1141:1158	the smallest value of Young's modulus	1141:1177	Fried Agria and Spunta, (56.3 and 61 kPa, respectively) had the smallest value of Young's modulus.					
30361005	2	25	dep	varieties	380:388	arg1	Agata					391:395	Agata	391:395	Agata	391:395	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	2	25	dep	varieties	380:388	arg1	Lady					416:419	Lady	416:419	Lady	416:419	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	2	25	dep	varieties	380:388	arg1	Innovator					405:413	Innovator	405:413	Innovator	405:413	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	4	26	theme	Differential	618:629	arg1	analysis					652:659	Differential Scanning Calorimeter analysis	618:659	Differential Scanning Calorimeter analysis	618:659	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	7	27	dep	Agria	1087:1091	arg1	61 kPa					1115:1120	61 kPa	1115:1120	61 kPa	1115:1120	Fried Agria and Spunta, (56.3 and 61 kPa, respectively) had the smallest value of Young's modulus.					
30361005	7	27	dep	Agria	1087:1091	arg1	56.3					1106:1109	56.3	1106:1109	56.3	1106:1109	Fried Agria and Spunta, (56.3 and 61 kPa, respectively) had the smallest value of Young's modulus.					
30361005	10	28	theme	phenotypic	1428:1437	arg1	traits					1439:1444	its exclusive phenotypic traits	1414:1444	its exclusive phenotypic traits	1414:1444	Such distance corresponded to its exclusive phenotypic traits, that are known to affect thermochemical properties.					
30361005	1	29	theme	cooked	170:175	arg1	potatoes					177:184	cooked potatoes	170:184	cooked potatoes in hot water, baked potatoes and the most popular fried potatoes	170:249	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	2	30	dep	Agata	391:395	arg1	Spunta					441:446	Spunta	441:446	Spunta	441:446	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	2	30	dep	Agata	391:395	arg1	Musica					430:435	Musica	430:435	Musica	430:435	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	7	31	contain	had	1137:1139	arg1	Spunta					1097:1102	Spunta	1097:1102	Spunta	1097:1102	Fried Agria and Spunta, (56.3 and 61 kPa, respectively) had the smallest value of Young's modulus.					
30361005	7	31	contain	had	1137:1139	arg1	Agria					1087:1091	Agria	1087:1091	Agria	1087:1091	Fried Agria and Spunta, (56.3 and 61 kPa, respectively) had the smallest value of Young's modulus.					
30361005	7	31	contain	had	1137:1139	arg2	value					1154:1158	the smallest value	1141:1158	the smallest value of Young's modulus	1141:1177	Fried Agria and Spunta, (56.3 and 61 kPa, respectively) had the smallest value of Young's modulus.					
30361005	1	32	theme	Potatoes	100:107	arg1	materials					128:136	the raw materials	120:136	the raw materials	120:136	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	1	32	theme	Potatoes	100:107	arg1	potatoes					177:184	cooked potatoes	170:184	cooked potatoes in hot water, baked potatoes and the most popular fried potatoes	170:249	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	1	32	theme	Potatoes	100:107	arg1	tubers					109:114	Potatoes tubers	100:114	Potatoes tubers	100:114	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	2	33	theme	frying	326:331	arg1	impact					296:301	the impact	292:301	the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin	292:469	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	10	34	theme	Such	1384:1387	arg1	distance					1389:1396	Such distance	1384:1396	Such distance	1384:1396	Such distance corresponded to its exclusive phenotypic traits, that are known to affect thermochemical properties.					
30361005	10	35	theme	thermochemical	1472:1485	arg1	properties					1487:1496	thermochemical properties	1472:1496	thermochemical properties	1472:1496	Such distance corresponded to its exclusive phenotypic traits, that are known to affect thermochemical properties.					
30361005	2	36	theme	work	274:277	arg1	objective					256:264	The objective	252:264	The objective of this work	252:277	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	2	37	theme	potato	373:378	arg1	varieties					380:388	six potato varieties	369:388	six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin	369:469	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	2	38	theme	varieties	380:388	arg1	microstructure					336:349	microstructure	336:349	microstructure	336:349	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	2	38	theme	varieties	380:388	arg1	properties					355:364	properties	355:364	properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin	355:469	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	6	39	theme	thermal	1062:1068	arg1	processes					1070:1078	thermal processes	1062:1078	thermal processes	1062:1078	In particular, baked Lady Rosetta (224.3 kPa) showed the least decrease in rigidity between thermal processes.					
30361005	1	40	theme	hot	189:191	arg1	water					193:197	hot water	189:197	hot water	189:197	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	5	41	theme	process	891:897	arg1	type					899:902	process type	891:902	process type	891:902	In addition, the elasticity modulus of cooked samples depended on process type and followed the order: baked potatoes > boiled > fried potatoes.					
30361005	4	42	theme	Calorimeter	640:650	arg1	analysis					652:659	Differential Scanning Calorimeter analysis	618:659	Differential Scanning Calorimeter analysis	618:659	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	3	43	theme	significant	510:520	arg1	differences					522:532	significant differences	510:532	significant differences between varieties and tuber microstructure changes following all cooking processes	510:615	Scanning Electron Microscopy revealed significant differences between varieties and tuber microstructure changes following all cooking processes.					
30361005	8	44	theme	marker	1190:1195	arg1	analyses					1197:1204	Molecular marker analyses	1180:1204	Molecular marker analyses	1180:1204	Molecular marker analyses provided a genetic fingerprinting of our varieties, allowing the identification of diagnostic markers.					
30361005	2	45	theme	different	454:462	arg1	origin					464:469	different origin	454:469	different origin	454:469	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	4	46	theme	tubers	789:794	arg1	2.1 J/g-3.9 J/g					769:783	2.1 J/g-3.9 J/g	769:783	2.1 J/g-3.9 J/g	769:783	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	4	46	theme	tubers	789:794	arg1	gelatinization					753:766	gelatinization	753:766	gelatinization (2.1 J/g-3.9 J/g) of tubers	753:794	Differential Scanning Calorimeter analysis showed that the transition temperatures (ranging between 60 °C and 85 °C) and enthalpies of gelatinization (2.1 J/g-3.9 J/g) of tubers were also variety dependent.					
30361005	9	47	theme	important	1331:1339	arg1	distance					1349:1356	an important genetic distance	1328:1356	an important genetic distance from the other varieties	1328:1381	Innovator revealed an important genetic distance from the other varieties.					
30361005	3	48	theme	cooking	599:605	arg1	processes					607:615	all cooking processes	595:615	all cooking processes	595:615	Scanning Electron Microscopy revealed significant differences between varieties and tuber microstructure changes following all cooking processes.					
30361005	2	49	with	varieties	380:388	arg1	origin					464:469	different origin	454:469	different origin	454:469	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30361005	8	50	theme	varieties	1247:1255	arg1	fingerprinting					1225:1238	a genetic fingerprinting	1215:1238	a genetic fingerprinting of our varieties, allowing the identification of diagnostic markers	1215:1306	Molecular marker analyses provided a genetic fingerprinting of our varieties, allowing the identification of diagnostic markers.					
30361005	0	51	theme	cooking	54:60	arg1	performances					62:73	cooking performances	54:73	cooking performances	54:73	Relationships between composition, microstructure and cooking performances of six potato varieties.					
30361005	9	52	theme	genetic	1341:1347	arg1	distance					1349:1356	an important genetic distance	1328:1356	an important genetic distance from the other varieties	1328:1381	Innovator revealed an important genetic distance from the other varieties.					
30361005	9	53	from	varieties	1373:1381	arg1	distance					1349:1356	an important genetic distance	1328:1356	an important genetic distance from the other varieties	1328:1381	Innovator revealed an important genetic distance from the other varieties.					
30361005	1	54	theme	raw	124:126	arg1	materials					128:136	the raw materials	120:136	the raw materials	120:136	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	1	54	theme	raw	124:126	arg1	potatoes					177:184	cooked potatoes	170:184	cooked potatoes in hot water, baked potatoes and the most popular fried potatoes	170:249	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	1	54	theme	raw	124:126	arg1	tubers					109:114	Potatoes tubers	100:114	Potatoes tubers	100:114	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	11	55	theme	appreciable	1605:1615	arg1	traits					1636:1641	appreciable food technological traits	1605:1641	appreciable food technological traits	1605:1641	The information obtained in this work may be useful to further study and associate genetic sequences with appreciable food technological traits.					
30361005	3	56	theme	Electron	481:488	arg1	Microscopy					490:499	Scanning Electron Microscopy	472:499	Scanning Electron Microscopy	472:499	Scanning Electron Microscopy revealed significant differences between varieties and tuber microstructure changes following all cooking processes.					
30361005	1	57	theme	baked	200:204	arg1	potatoes					206:213	baked potatoes	200:213	baked potatoes	200:213	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	6	58	theme	least	1027:1031	arg1	decrease					1033:1040	the least decrease	1023:1040	the least decrease in rigidity between thermal processes	1023:1078	In particular, baked Lady Rosetta (224.3 kPa) showed the least decrease in rigidity between thermal processes.					
30361005	1	59	theme	processed	146:154	arg1	food					156:159	many processed food	141:159	many processed food	141:159	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	8	60	theme	markers	1300:1306	arg1	identification					1271:1284	the identification	1267:1284	the identification of diagnostic markers	1267:1306	Molecular marker analyses provided a genetic fingerprinting of our varieties, allowing the identification of diagnostic markers.					
30361005	11	61	with	sequences	1590:1598	arg1	traits					1636:1641	appreciable food technological traits	1605:1641	appreciable food technological traits	1605:1641	The information obtained in this work may be useful to further study and associate genetic sequences with appreciable food technological traits.					
30361005	5	62	theme	cooked	864:869	arg1	samples					871:877	cooked samples	864:877	cooked samples	864:877	In addition, the elasticity modulus of cooked samples depended on process type and followed the order: baked potatoes > boiled > fried potatoes.					
30361005	3	63	theme	tuber	556:560	arg1	changes					577:583	tuber microstructure changes	556:583	tuber microstructure changes	556:583	Scanning Electron Microscopy revealed significant differences between varieties and tuber microstructure changes following all cooking processes.					
30361005	1	64	from	potatoes	177:184	arg1	water					193:197	hot water	189:197	hot water	189:197	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	1	64	from	potatoes	177:184	arg1	potatoes					206:213	baked potatoes	200:213	baked potatoes	200:213	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	1	64	from	potatoes	177:184	arg1	potatoes					242:249	the most popular fried potatoes	219:249	the most popular fried potatoes	219:249	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	1	65	theme	many	141:144	arg1	food					156:159	many processed food	141:159	many processed food	141:159	Potatoes tubers are the raw materials of many processed food, such as cooked potatoes in hot water, baked potatoes and the most popular fried potatoes.					
30361005	8	66	theme	Molecular	1180:1188	arg1	analyses					1197:1204	Molecular marker analyses	1180:1204	Molecular marker analyses	1180:1204	Molecular marker analyses provided a genetic fingerprinting of our varieties, allowing the identification of diagnostic markers.					
30361005	9	67	theme	other	1367:1371	arg1	varieties					1373:1381	the other varieties	1363:1381	the other varieties	1363:1381	Innovator revealed an important genetic distance from the other varieties.					
30361005	3	68	theme	microstructure	562:575	arg1	changes					577:583	tuber microstructure changes	556:583	tuber microstructure changes	556:583	Scanning Electron Microscopy revealed significant differences between varieties and tuber microstructure changes following all cooking processes.					
30361005	11	69	theme	genetic	1582:1588	arg1	sequences					1590:1598	genetic sequences	1582:1598	genetic sequences with appreciable food technological traits	1582:1641	The information obtained in this work may be useful to further study and associate genetic sequences with appreciable food technological traits.					
30361005	2	70	theme	baking	315:320	arg1	impact					296:301	the impact	292:301	the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin	292:469	The objective of this work was to study the impact of boiling, baking and frying on microstructure and properties of six potato varieties (Agata, Agria, Innovator, Lady Rosetta, Musica and Spunta) with different origin.					
30609039	7	0	theme	modified	1325:1332	arg1	films					1341:1345	modified starch films	1325:1345	modified starch films	1325:1345	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	2	1	theme	Dual-modified	369:381	arg1	films					414:418	Dual-modified lotus rhizome starch composite films	369:418	Dual-modified lotus rhizome starch composite films	369:418	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	5	2	located	observed	1041:1048	arg2	troughs					1028:1034	troughs	1028:1034	troughs	1028:1034	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	5	2	located	observed	1041:1048	arg1	films					1100:1104	crosslinked and dual-modified starch composite films	1053:1104	crosslinked and dual-modified starch composite films	1053:1104	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	3	3	theme	composite	601:609	arg1	films					611:615	dual-modified starch composite films	580:615	dual-modified starch composite films (FLCOS1 , FLCOS2 )	580:634	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	9	4	theme	Chemical	1783:1790	arg1	Industry					1792:1799	Chemical Industry	1783:1799	Chemical Industry	1783:1799	© 2019 Society of Chemical Industry.					
30609039	3	5	theme	dual-modified	580:592	arg1	films					611:615	dual-modified starch composite films	580:615	dual-modified starch composite films (FLCOS1 , FLCOS2 )	580:634	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	5	6	link	crosslinked	1053:1063	arg1	films					1100:1104	crosslinked and dual-modified starch composite films	1053:1104	crosslinked and dual-modified starch composite films	1053:1104	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	6	7	theme	compact	1220:1226	arg1	microstructures					1228:1242	compact microstructures	1220:1242	compact microstructures	1220:1242	Using whey protein concentrate, psyllium husk and glycerol without any phase separation, smoother films and with compact microstructures were produced.					
30609039	3	8	dep	found	640:644	arg1	whereas					663:669	whereas	663:669	whereas	663:669	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	8	9	theme	modified	1573:1580	arg1	starches					1582:1589	modified starches	1573:1589	modified starches	1573:1589	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	7	10	theme	starch	1506:1511	arg1	films					1523:1527	modified starch composite films	1497:1527	modified starch composite films	1497:1527	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	4	11	dep	transparent	768:778	arg1	whereas					830:836	whereas	830:836	whereas	830:836	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	1	12	theme	protein	265:271	arg1	concentrate					273:283	whey protein concentrate	260:283	whey protein concentrate	260:283	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	1	13	theme	modified	229:236	arg1	starch					252:257	variously chemically modified lotus rhizome starch	208:257	variously chemically modified lotus rhizome starch	208:257	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	6	14	with	films	1205:1209	arg1	microstructures					1228:1242	compact microstructures	1220:1242	compact microstructures	1220:1242	Using whey protein concentrate, psyllium husk and glycerol without any phase separation, smoother films and with compact microstructures were produced.					
30609039	0	15	from	protein	66:72	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	0	15	from	protein	66:72	arg1	films					50:54	biodegradable films	36:54	biodegradable films from whey protein	36:72	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	0	15	from	protein	66:72	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	7	16	theme	diffraction	1474:1484	arg1	results					1486:1492	X-ray diffraction results	1468:1492	X-ray diffraction results of modified starch composite films	1468:1527	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	4	17	theme	composite	738:746	arg1	transparent					768:778	transparent	768:778	transparent	768:778	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	17	theme	composite	738:746	arg1	films					748:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	0	18	theme	rhizome	148:154	arg1	composite					163:171	oxidized, crosslinked, dual-modified lotus rhizome starch composite	105:171	oxidized, crosslinked, dual-modified lotus rhizome starch composite	105:171	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	5	19	theme	compact	968:974	arg1	surface					976:982	a homogeneous compact surface	954:982	a homogeneous compact surface of oxidized starch composite films	954:1017	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	7	20	theme	film-forming	1396:1407	arg1	components					1409:1418	starch and film-forming components	1385:1418	starch and film-forming components	1385:1418	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	4	21	theme	Oxidized	708:715	arg1	transparent					768:778	transparent	768:778	transparent	768:778	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	21	theme	Oxidized	708:715	arg1	films					748:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	1	22	theme	biodegradable	340:352	arg1	films					354:358	developed biodegradable films	330:358	developed biodegradable films	330:358	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	2	23	theme	starch	533:538	arg1	films					550:554	native and modified starch composite films	513:554	native and modified starch composite films	513:554	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	0	24	from	characterization	16:31	arg1	protein					66:72	whey protein	61:72	whey protein	61:72	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	4	25	theme	rhizome	723:729	arg1	transparent					768:778	transparent	768:778	transparent	768:778	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	25	theme	rhizome	723:729	arg1	films					748:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	7	26	theme	additional	1304:1313	arg1	peaks					1315:1319	additional peaks	1304:1319	additional peaks for modified starch films	1304:1345	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	0	27	from	Development	0:10	arg1	protein					66:72	whey protein	61:72	whey protein	61:72	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	0	28	dep	oxidized	105:112	arg1	dual-modified					128:140	dual-modified	128:140	dual-modified	128:140	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	0	28	dep	oxidized	105:112	arg1	crosslinked					115:125	crosslinked	115:125	crosslinked	115:125	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	4	29	theme	starch	806:811	arg1	films					823:827	native and modified starch composite films	786:827	native and modified starch composite films	786:827	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	2	30	theme	minimum	430:436	arg1	solubility					438:447	minimum solubility	430:447	minimum solubility	430:447	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	0	31	theme	oxidized	105:112	arg1	composite					163:171	oxidized, crosslinked, dual-modified lotus rhizome starch composite	105:171	oxidized, crosslinked, dual-modified lotus rhizome starch composite	105:171	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	6	32	theme	psyllium	1139:1146	arg1	husk					1148:1151	psyllium husk	1139:1151	psyllium husk	1139:1151	Using whey protein concentrate, psyllium husk and glycerol without any phase separation, smoother films and with compact microstructures were produced.					
30609039	2	33	theme	tensile	490:496	arg1	strength					498:505	maximum tensile strength	482:505	maximum tensile strength	482:505	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	5	34	dep	indicated	944:952	arg1	whereas					1020:1026	whereas	1020:1026	whereas	1020:1026	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	1	35	theme	psyllium	286:293	arg1	husk					295:298	psyllium husk	286:298	psyllium husk	286:298	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	5	36	theme	starch	996:1001	arg1	films					1013:1017	oxidized starch composite films	987:1017	oxidized starch composite films	987:1017	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	4	37	link	crosslinked	838:848	arg1	transparent					902:912	transparent	902:912	transparent	902:912	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	37	link	crosslinked	838:848	arg1	films					881:885	crosslinked lotus rhizome starch composite films	838:885	crosslinked lotus rhizome starch composite films	838:885	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	2	38	dep	RESULTS	361:367	arg1	presented					420:428	presented	420:428	presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films	420:554	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	2	39	theme	vapor	459:463	arg1	permeability					465:476	water vapor permeability	453:476	water vapor permeability	453:476	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	8	40	from	application	1609:1619	arg1	packages					1639:1646	edible contact packages	1624:1646	edible contact packages	1624:1646	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	5	41	theme	films	1013:1017	arg1	surface					976:982	a homogeneous compact surface	954:982	a homogeneous compact surface of oxidized starch composite films	954:1017	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	6	42	theme	whey	1113:1116	arg1	concentrate					1126:1136	whey protein concentrate	1113:1136	whey protein concentrate	1113:1136	Using whey protein concentrate, psyllium husk and glycerol without any phase separation, smoother films and with compact microstructures were produced.					
30609039	4	43	theme	lotus	850:854	arg1	transparent					902:912	transparent	902:912	transparent	902:912	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	43	theme	lotus	850:854	arg1	films					881:885	crosslinked lotus rhizome starch composite films	838:885	crosslinked lotus rhizome starch composite films	838:885	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	0	44	theme	biodegradable	36:48	arg1	films					50:54	biodegradable films	36:54	biodegradable films from whey protein	36:72	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	5	45	theme	starch	1083:1088	arg1	films					1100:1104	crosslinked and dual-modified starch composite films	1053:1104	crosslinked and dual-modified starch composite films	1053:1104	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	8	46	used	used	1666:1669	arg2	films					1598:1602	these films	1592:1602	these films	1592:1602	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	4	47	theme	starch	864:869	arg1	transparent					902:912	transparent	902:912	transparent	902:912	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	47	theme	starch	864:869	arg1	films					881:885	crosslinked lotus rhizome starch composite films	838:885	crosslinked lotus rhizome starch composite films	838:885	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	8	48	theme	structural	1697:1706	arg1	integrity					1708:1716	higher structural integrity	1690:1716	higher structural integrity	1690:1716	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	1	49	theme	lotus	238:242	arg1	starch					252:257	variously chemically modified lotus rhizome starch	208:257	variously chemically modified lotus rhizome starch	208:257	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	6	50	theme	phase	1178:1182	arg1	separation					1184:1193	any phase separation	1174:1193	any phase separation	1174:1193	Using whey protein concentrate, psyllium husk and glycerol without any phase separation, smoother films and with compact microstructures were produced.					
30609039	8	51	theme	various	1551:1557	arg1	properties					1559:1568	various properties	1551:1568	various properties of modified starches	1551:1589	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	3	52	dep	films	611:615	arg1	FLCOS2					627:632	FLCOS2	627:632	FLCOS2	627:632	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	3	52	dep	films	611:615	arg1	FLCOS1					618:623	FLCOS1	618:623	FLCOS1	618:623	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	2	53	theme	starch	397:402	arg1	films					414:418	Dual-modified lotus rhizome starch composite films	369:418	Dual-modified lotus rhizome starch composite films	369:418	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	1	54	theme	starch	252:257	arg1	effect					198:203	The combined effect	185:203	The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol	185:311	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	8	55	theme	water	1728:1732	arg1	transmission					1740:1751	lower water vapor transmission	1722:1751	lower water vapor transmission	1722:1751	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	2	56	theme	lotus	383:387	arg1	films					414:418	Dual-modified lotus rhizome starch composite films	369:418	Dual-modified lotus rhizome starch composite films	369:418	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	1	57	theme	whey	260:263	arg1	concentrate					273:283	whey protein concentrate	260:283	whey protein concentrate	260:283	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	7	58	theme	starch	1334:1339	arg1	films					1341:1345	modified starch films	1325:1345	modified starch films	1325:1345	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	1	59	dep	BACKGROUND	174:183	arg1	evaluated					317:325	evaluated	317:325	was evaluated on developed biodegradable films	313:358	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	3	60	theme	starch	594:599	arg1	films					611:615	dual-modified starch composite films	580:615	dual-modified starch composite films (FLCOS1 , FLCOS2 )	580:634	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	1	61	theme	concentrate	273:283	arg1	effect					198:203	The combined effect	185:203	The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol	185:311	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	7	62	theme	films	1523:1527	arg1	results					1486:1492	X-ray diffraction results	1468:1492	X-ray diffraction results of modified starch composite films	1468:1527	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	3	63	from	break	571:575	arg1	Elongation					557:566	Elongation	557:566	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 )	557:634	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	3	63	from	break	571:575	arg1	maximum					654:660	a maximum	652:660	a maximum	652:660	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	4	64	theme	composite	871:879	arg1	transparent					902:912	transparent	902:912	transparent	902:912	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	64	theme	composite	871:879	arg1	films					881:885	crosslinked lotus rhizome starch composite films	838:885	crosslinked lotus rhizome starch composite films	838:885	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	1	65	theme	combined	189:196	arg1	effect					198:203	The combined effect	185:203	The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol	185:311	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	3	66	theme	films	611:615	arg1	Elongation					557:566	Elongation	557:566	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 )	557:634	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	3	66	theme	films	611:615	arg1	maximum					654:660	a maximum	652:660	a maximum	652:660	Elongation at break of dual-modified starch composite films (FLCOS1 , FLCOS2 ) was found to be a maximum, whereas a decrease was observed for FLCOS3 .					
30609039	7	67	theme	composite	1513:1521	arg1	films					1523:1527	modified starch composite films	1497:1527	modified starch composite films	1497:1527	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	0	68	theme	lotus	142:146	arg1	composite					163:171	oxidized, crosslinked, dual-modified lotus rhizome starch composite	105:171	oxidized, crosslinked, dual-modified lotus rhizome starch composite	105:171	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	5	69	theme	Scanning	915:922	arg1	microscopy					933:942	Scanning electron microscopy	915:942	Scanning electron microscopy	915:942	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	8	70	theme	starches	1582:1589	arg1	properties					1559:1568	various properties	1551:1568	various properties of modified starches	1551:1589	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	7	71	theme	modified	1497:1504	arg1	films					1523:1527	modified starch composite films	1497:1527	modified starch composite films	1497:1527	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	0	72	theme	starch	156:161	arg1	composite					163:171	oxidized, crosslinked, dual-modified lotus rhizome starch composite	105:171	oxidized, crosslinked, dual-modified lotus rhizome starch composite	105:171	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	5	73	theme	homogeneous	956:966	arg1	surface					976:982	a homogeneous compact surface	954:982	a homogeneous compact surface of oxidized starch composite films	954:1017	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	8	74	theme	edible	1624:1629	arg1	packages					1639:1646	edible contact packages	1624:1646	edible contact packages	1624:1646	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	4	75	theme	starch	731:736	arg1	transparent					768:778	transparent	768:778	transparent	768:778	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	75	theme	starch	731:736	arg1	films					748:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	7	76	theme	X-ray	1468:1472	arg1	results					1486:1492	X-ray diffraction results	1468:1492	X-ray diffraction results of modified starch composite films	1468:1527	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	5	77	theme	electron	924:931	arg1	microscopy					933:942	Scanning electron microscopy	915:942	Scanning electron microscopy	915:942	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	2	78	theme	composite	540:548	arg1	films					550:554	native and modified starch composite films	513:554	native and modified starch composite films	513:554	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	0	79	theme	psyllium	87:94	arg1	husk					96:99	psyllium husk	87:99	psyllium husk	87:99	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	2	80	theme	modified	524:531	arg1	films					550:554	native and modified starch composite films	513:554	native and modified starch composite films	513:554	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	8	81	theme	contact	1631:1637	arg1	packages					1639:1646	edible contact packages	1624:1646	edible contact packages	1624:1646	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	4	82	theme	lotus	717:721	arg1	transparent					768:778	transparent	768:778	transparent	768:778	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	82	theme	lotus	717:721	arg1	films					748:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films	708:752	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	7	83	theme	infrared	1277:1284	arg1	analysis					1286:1293	infrared analysis	1277:1293	infrared analysis	1277:1293	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	4	84	theme	native	786:791	arg1	films					823:827	native and modified starch composite films	786:827	native and modified starch composite films	786:827	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	7	85	theme	greater	1359:1365	arg1	interaction					1367:1377	greater interaction	1359:1377	greater interaction among starch and film-forming components	1359:1418	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	7	86	theme	amorphous	1429:1437	arg1	structure					1439:1447	amorphous structure	1429:1447	amorphous structure	1429:1447	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	4	87	theme	modified	797:804	arg1	films					823:827	native and modified starch composite films	786:827	native and modified starch composite films	786:827	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	5	88	theme	oxidized	987:994	arg1	films					1013:1017	oxidized starch composite films	987:1017	oxidized starch composite films	987:1017	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	2	89	theme	maximum	482:488	arg1	strength					498:505	maximum tensile strength	482:505	maximum tensile strength	482:505	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	2	90	theme	composite	404:412	arg1	films					414:418	Dual-modified lotus rhizome starch composite films	369:418	Dual-modified lotus rhizome starch composite films	369:418	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	1	91	theme	husk	295:298	arg1	effect					198:203	The combined effect	185:203	The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol	185:311	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	5	92	theme	composite	1003:1011	arg1	films					1013:1017	oxidized starch composite films	987:1017	oxidized starch composite films	987:1017	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	6	93	theme	protein	1118:1124	arg1	concentrate					1126:1136	whey protein concentrate	1113:1136	whey protein concentrate	1113:1136	Using whey protein concentrate, psyllium husk and glycerol without any phase separation, smoother films and with compact microstructures were produced.					
30609039	2	94	theme	water	453:457	arg1	permeability					465:476	water vapor permeability	453:476	water vapor permeability	453:476	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	0	95	theme	films	50:54	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	0	95	theme	films	50:54	arg1	Development					0:10	Development	0:10	Development	0:10	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	1	96	theme	glycerol	304:311	arg1	effect					198:203	The combined effect	185:203	The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol	185:311	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	5	97	theme	dual-modified	1069:1081	arg1	films					1100:1104	crosslinked and dual-modified starch composite films	1053:1104	crosslinked and dual-modified starch composite films	1053:1104	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	7	98	theme	starch	1385:1390	arg1	components					1409:1418	starch and film-forming components	1385:1418	starch and film-forming components	1385:1418	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	2	99	theme	native	513:518	arg1	films					550:554	native and modified starch composite films	513:554	native and modified starch composite films	513:554	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
30609039	8	100	theme	higher	1690:1695	arg1	integrity					1708:1716	higher structural integrity	1690:1716	higher structural integrity	1690:1716	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	4	101	theme	crosslinked	838:848	arg1	transparent					902:912	transparent	902:912	transparent	902:912	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	101	theme	crosslinked	838:848	arg1	films					881:885	crosslinked lotus rhizome starch composite films	838:885	crosslinked lotus rhizome starch composite films	838:885	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	0	102	theme	whey	61:64	arg1	protein					66:72	whey protein	61:72	whey protein	61:72	Development and characterization of biodegradable films from whey protein concentrate, psyllium husk and oxidized, crosslinked, dual-modified lotus rhizome starch composite.					
30609039	5	103	theme	composite	1090:1098	arg1	films					1100:1104	crosslinked and dual-modified starch composite films	1053:1104	crosslinked and dual-modified starch composite films	1053:1104	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	6	104	theme	smoother	1196:1203	arg1	films					1205:1209	smoother films	1196:1209	smoother films and with compact microstructures	1196:1242	Using whey protein concentrate, psyllium husk and glycerol without any phase separation, smoother films and with compact microstructures were produced.					
30609039	7	105	dep	transform	1267:1275	arg1	whereas					1421:1427	whereas	1421:1427	whereas	1421:1427	Fourier transform infrared analysis revealed additional peaks for modified starch films, confirming greater interaction among starch and film-forming components, whereas amorphous structure was indicated from X-ray diffraction results of modified starch composite films.					
30609039	4	106	theme	rhizome	856:862	arg1	transparent					902:912	transparent	902:912	transparent	902:912	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	4	106	theme	rhizome	856:862	arg1	films					881:885	crosslinked lotus rhizome starch composite films	838:885	crosslinked lotus rhizome starch composite films	838:885	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	1	107	theme	developed	330:338	arg1	films					354:358	developed biodegradable films	330:358	developed biodegradable films	330:358	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	8	108	theme	lower	1722:1726	arg1	transmission					1740:1751	lower water vapor transmission	1722:1751	lower water vapor transmission	1722:1751	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	4	109	theme	composite	813:821	arg1	films					823:827	native and modified starch composite films	786:827	native and modified starch composite films	786:827	Oxidized lotus rhizome starch composite films were the most transparent among native and modified starch composite films, whereas crosslinked lotus rhizome starch composite films were the least transparent.					
30609039	1	110	theme	rhizome	244:250	arg1	starch					252:257	variously chemically modified lotus rhizome starch	208:257	variously chemically modified lotus rhizome starch	208:257	BACKGROUND The combined effect of variously chemically modified lotus rhizome starch, whey protein concentrate, psyllium husk and glycerol was evaluated on developed biodegradable films.					
30609039	5	111	theme	crosslinked	1053:1063	arg1	films					1100:1104	crosslinked and dual-modified starch composite films	1053:1104	crosslinked and dual-modified starch composite films	1053:1104	Scanning electron microscopy indicated a homogeneous compact surface of oxidized starch composite films, whereas troughs were observed in crosslinked and dual-modified starch composite films.					
30609039	8	112	theme	vapor	1734:1738	arg1	transmission					1740:1751	lower water vapor transmission	1722:1751	lower water vapor transmission	1722:1751	CONCLUSIONS Owing to various properties of modified starches, these films find application in edible contact packages and can better be used for products where higher structural integrity and lower water vapor transmission are needed.					
30609039	2	113	theme	rhizome	389:395	arg1	films					414:418	Dual-modified lotus rhizome starch composite films	369:418	Dual-modified lotus rhizome starch composite films	369:418	RESULTS Dual-modified lotus rhizome starch composite films presented minimum solubility and water vapor permeability and maximum tensile strength among native and modified starch composite films.					
31279883	6	0	dep	%	1166:1166	arg1	89					1164:1165	89	1164:1165	89	1164:1165	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	7	1	theme	industrial	1361:1370	arg1	wastewater					1372:1381	industrial wastewater	1361:1381	industrial wastewater	1361:1381	This easily recoverable floating photocatalyst may be applied to improve the photocatalytic treatment of industrial wastewater, making most efficient use of photoactivation with no mechanical stirring.					
31279883	3	2	theme	surface	670:676	arg1	methodology					678:688	the response surface methodology	657:688	the response surface methodology	657:688	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	3	3	theme	CaCO3	476:480	arg1	concentrations					495:508	The TiO2, CaCO3, and CH3COOH concentrations	466:508	The TiO2, CaCO3, and CH3COOH concentrations	466:508	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	7	4	theme	mechanical	1437:1446	arg1	stirring					1448:1455	no mechanical stirring	1434:1455	no mechanical stirring	1434:1455	This easily recoverable floating photocatalyst may be applied to improve the photocatalytic treatment of industrial wastewater, making most efficient use of photoactivation with no mechanical stirring.					
31279883	6	5	theme	use	1244:1246	arg1	cycles					1248:1253	seven use cycles	1238:1253	seven use cycles	1238:1253	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	7	6	theme	photocatalytic	1333:1346	arg1	treatment					1348:1356	the photocatalytic treatment	1329:1356	the photocatalytic treatment of industrial wastewater	1329:1381	This easily recoverable floating photocatalyst may be applied to improve the photocatalytic treatment of industrial wastewater, making most efficient use of photoactivation with no mechanical stirring.					
31279883	6	7	dep	discoloration	1168:1180	arg1	%					1166:1166	%	1166:1166	%	1166:1166	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	3	8	dep	response	540:547	arg1	%					553:553	100%	550:553	100% of floating beads	550:571	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	3	8	dep	response	540:547	arg1	beads					567:571	floating beads	558:571	floating beads	558:571	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	4	9	theme	vt	816:817	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	9	theme	vt	816:817	arg1	CH3COOH					820:826	6.4 vt% CH3COOH	812:826	6.4 vt% CH3COOH	812:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	10	theme	optimal	713:719	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	10	theme	optimal	713:719	arg1	formulation					732:742	the optimal TiO2/CaAlg formulation	709:742	the optimal TiO2/CaAlg formulation	709:742	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	1	11	theme	easier	258:263	arg1	separation					280:289	easier post-treatment separation	258:289	easier post-treatment separation	258:289	For the first time, TiO2 immobilized in alginate polymer was modified to turn it into a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation.					
31279883	3	12	theme	best	535:538	arg1	response					540:547	the best response	531:547	the best response (100% of floating beads) after 24 h	531:583	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	5	13	dep	uniform	863:869	arg1	size					872:875	size	872:875	size	872:875	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	5	13	dep	uniform	863:869	arg1	uniform					863:869	uniform	863:869	uniform	863:869	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	5	13	dep	uniform	863:869	arg1	beads					852:856	The resulting floating beads	829:856	The resulting floating beads	829:856	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	1	14	theme	post-treatment	265:278	arg1	separation					280:289	easier post-treatment separation	258:289	easier post-treatment separation	258:289	For the first time, TiO2 immobilized in alginate polymer was modified to turn it into a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation.					
31279883	6	15	theme	removal	1210:1216	arg1	capacity					1218:1225	TOC removal capacity	1206:1225	TOC removal capacity	1206:1225	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	5	16	theme	resulting	833:841	arg1	size					872:875	size	872:875	size	872:875	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	5	16	theme	resulting	833:841	arg1	uniform					863:869	uniform	863:869	uniform	863:869	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	5	16	theme	resulting	833:841	arg1	beads					852:856	The resulting floating beads	829:856	The resulting floating beads	829:856	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	4	17	theme	2.3 wt	793:798	arg1	CaCO3					801:805	2.3 wt% CaCO3	793:805	2.3 wt% CaCO3	793:805	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	17	theme	2.3 wt	793:798	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	7	18	theme	recoverable	1268:1278	arg1	photocatalyst					1289:1301	This easily recoverable floating photocatalyst	1256:1301	This easily recoverable floating photocatalyst	1256:1301	This easily recoverable floating photocatalyst may be applied to improve the photocatalytic treatment of industrial wastewater, making most efficient use of photoactivation with no mechanical stirring.					
31279883	7	19	theme	floating	1280:1287	arg1	photocatalyst					1289:1301	This easily recoverable floating photocatalyst	1256:1301	This easily recoverable floating photocatalyst	1256:1301	This easily recoverable floating photocatalyst may be applied to improve the photocatalytic treatment of industrial wastewater, making most efficient use of photoactivation with no mechanical stirring.					
31279883	4	20	dep	concentrations	763:776	arg1	CaCO3					801:805	2.3 wt% CaCO3	793:805	2.3 wt% CaCO3	793:805	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	20	dep	concentrations	763:776	arg1	CH3COOH					820:826	6.4 vt% CH3COOH	812:826	6.4 vt% CH3COOH	812:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	20	dep	concentrations	763:776	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	20	dep	concentrations	763:776	arg1	TiO2					787:790	1.1 wt% TiO2	779:790	1.1 wt% TiO2	779:790	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	6	21	theme	TOC	1206:1208	arg1	capacity					1218:1225	TOC removal capacity	1206:1225	TOC removal capacity	1206:1225	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	3	22	theme	response	661:668	arg1	methodology					678:688	the response surface methodology	657:688	the response surface methodology	657:688	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	6	23	theme	dye	1135:1137	arg1	removal					1139:1145	tartrazine dye removal	1124:1145	tartrazine dye removal	1124:1145	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	4	24	theme	TiO2/CaAlg	721:730	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	24	theme	TiO2/CaAlg	721:730	arg1	formulation					732:742	the optimal TiO2/CaAlg formulation	709:742	the optimal TiO2/CaAlg formulation	709:742	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	2	25	theme	gas-forming	403:413	arg1	agent					415:419	a gas-forming agent	401:419	a gas-forming agent (CaCO3, NaHCO3)	401:435	In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH).					
31279883	2	26	theme	acid	443:446	arg1	CH3COOH					456:462	CH3COOH	456:462	CH3COOH	456:462	In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH).					
31279883	2	26	theme	acid	443:446	arg1	medium					448:453	an acid medium	440:453	an acid medium (CH3COOH)	440:463	In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH).					
31279883	1	27	theme	alginate	126:133	arg1	polymer					135:141	alginate polymer	126:141	alginate polymer	126:141	For the first time, TiO2 immobilized in alginate polymer was modified to turn it into a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation.					
31279883	6	28	theme	tartrazine	1124:1133	arg1	removal					1139:1145	tartrazine dye removal	1124:1145	tartrazine dye removal	1124:1145	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	2	29	theme	low-density	310:320	arg1	photocatalyst					342:354	the low-density TiO2 alginate-based photocatalyst	306:354	the low-density TiO2 alginate-based photocatalyst	306:354	In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH).					
31279883	6	30	with	use	1197:1199	arg1	capacity					1218:1225	TOC removal capacity	1206:1225	TOC removal capacity	1206:1225	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	6	31	dep	89	1164:1165	arg1	to					1161:1162	to	1161:1162	to	1161:1162	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	4	32	theme	%	799:799	arg1	CaCO3					801:805	2.3 wt% CaCO3	793:805	2.3 wt% CaCO3	793:805	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	32	theme	%	799:799	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	1	33	theme	first	94:98	arg1	time					100:103	the first time	90:103	the first time	90:103	For the first time, TiO2 immobilized in alginate polymer was modified to turn it into a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation.					
31279883	5	34	with	uniform	863:869	arg1	structure					957:965	porous internal structure	941:965	porous internal structure	941:965	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	5	34	with	uniform	863:869	arg1	surface					929:935	a rough surface	921:935	a rough surface	921:935	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	3	35	theme	floating	558:565	arg1	beads					567:571	floating beads	558:571	floating beads	558:571	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	5	36	theme	porous	941:946	arg1	structure					957:965	porous internal structure	941:965	porous internal structure	941:965	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	7	37	with	use	1406:1408	arg1	stirring					1448:1455	no mechanical stirring	1434:1455	no mechanical stirring	1434:1455	This easily recoverable floating photocatalyst may be applied to improve the photocatalytic treatment of industrial wastewater, making most efficient use of photoactivation with no mechanical stirring.					
31279883	1	38	theme	buoyant	174:180	arg1	photocatalyst					182:194	a buoyant photocatalyst	172:194	a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation	172:289	For the first time, TiO2 immobilized in alginate polymer was modified to turn it into a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation.					
31279883	3	39	theme	CH3COOH	487:493	arg1	concentrations					495:508	The TiO2, CaCO3, and CH3COOH concentrations	466:508	The TiO2, CaCO3, and CH3COOH concentrations	466:508	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	0	40	theme	novel	34:38	arg1	photocatalyst					71:83	a novel TiO2/calcium alginate floating photocatalyst	32:83	a novel TiO2/calcium alginate floating photocatalyst	32:83	Formulation and optimization of a novel TiO2/calcium alginate floating photocatalyst.					
31279883	6	41	dep	irradiation	1078:1088	arg1	125 W					1091:1095	125 W	1091:1095	125 W	1091:1095	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	6	41	dep	irradiation	1078:1088	arg1	254 nm					1098:1103	254 nm	1098:1103	254 nm	1098:1103	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	6	42	theme	first	1191:1195	arg1	use					1197:1199	the first use	1187:1199	the first use with TOC removal capacity	1187:1225	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	7	43	theme	photoactivation	1413:1427	arg1	use					1406:1408	most efficient use	1391:1408	most efficient use of photoactivation with no mechanical stirring	1391:1455	This easily recoverable floating photocatalyst may be applied to improve the photocatalytic treatment of industrial wastewater, making most efficient use of photoactivation with no mechanical stirring.					
31279883	4	44	theme	1.1 wt	779:784	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	44	theme	1.1 wt	779:784	arg1	TiO2					787:790	1.1 wt% TiO2	779:790	1.1 wt% TiO2	779:790	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	7	45	theme	efficient	1396:1404	arg1	use					1406:1408	most efficient use	1391:1408	most efficient use of photoactivation with no mechanical stirring	1391:1455	This easily recoverable floating photocatalyst may be applied to improve the photocatalytic treatment of industrial wastewater, making most efficient use of photoactivation with no mechanical stirring.					
31279883	5	46	theme	rough	923:927	arg1	surface					929:935	a rough surface	921:935	a rough surface	921:935	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	0	47	theme	alginate	53:60	arg1	photocatalyst					71:83	a novel TiO2/calcium alginate floating photocatalyst	32:83	a novel TiO2/calcium alginate floating photocatalyst	32:83	Formulation and optimization of a novel TiO2/calcium alginate floating photocatalyst.					
31279883	5	48	theme	internal	948:955	arg1	structure					957:965	porous internal structure	941:965	porous internal structure	941:965	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	3	49	theme	TiO2	470:473	arg1	concentrations					495:508	The TiO2, CaCO3, and CH3COOH concentrations	466:508	The TiO2, CaCO3, and CH3COOH concentrations	466:508	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	0	50	theme	TiO2/calcium	40:51	arg1	photocatalyst					71:83	a novel TiO2/calcium alginate floating photocatalyst	32:83	a novel TiO2/calcium alginate floating photocatalyst	32:83	Formulation and optimization of a novel TiO2/calcium alginate floating photocatalyst.					
31279883	4	51	theme	%	818:818	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	51	theme	%	818:818	arg1	CH3COOH					820:826	6.4 vt% CH3COOH	812:826	6.4 vt% CH3COOH	812:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	3	52	theme	composite	616:624	arg1	design					636:641	a central composite rotatable design	606:641	a central composite rotatable design combined with the response surface methodology	606:688	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	4	53	theme	%	785:785	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	53	theme	%	785:785	arg1	TiO2					787:790	1.1 wt% TiO2	779:790	1.1 wt% TiO2	779:790	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	5	54	theme	floating	843:850	arg1	size					872:875	size	872:875	size	872:875	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	5	54	theme	floating	843:850	arg1	uniform					863:869	uniform	863:869	uniform	863:869	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	5	54	theme	floating	843:850	arg1	beads					852:856	The resulting floating beads	829:856	The resulting floating beads	829:856	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	4	55	theme	following	753:761	arg1	CaCO3					801:805	2.3 wt% CaCO3	793:805	2.3 wt% CaCO3	793:805	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	55	theme	following	753:761	arg1	concentrations					763:776	the following concentrations	749:776	the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH	749:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	55	theme	following	753:761	arg1	CH3COOH					820:826	6.4 vt% CH3COOH	812:826	6.4 vt% CH3COOH	812:826	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	55	theme	following	753:761	arg1	formulation					732:742	the optimal TiO2/CaAlg formulation	709:742	the optimal TiO2/CaAlg formulation	709:742	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	4	55	theme	following	753:761	arg1	TiO2					787:790	1.1 wt% TiO2	779:790	1.1 wt% TiO2	779:790	It was found that the optimal TiO2/CaAlg formulation were the following concentrations: 1.1 wt% TiO2, 2.3 wt% CaCO3, and 6.4 vt% CH3COOH.					
31279883	3	56	theme	rotatable	626:634	arg1	design					636:641	a central composite rotatable design	606:641	a central composite rotatable design combined with the response surface methodology	606:688	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	2	57	theme	alginate-based	327:340	arg1	photocatalyst					342:354	the low-density TiO2 alginate-based photocatalyst	306:354	the low-density TiO2 alginate-based photocatalyst	306:354	In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH).					
31279883	6	58	theme	photocatalytic	1045:1058	arg1	activity					1060:1067	The photocatalytic activity	1041:1067	The photocatalytic activity under UV irradiation (125 W, 254 nm)	1041:1104	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	7	59	theme	wastewater	1372:1381	arg1	treatment					1348:1356	the photocatalytic treatment	1329:1356	the photocatalytic treatment of industrial wastewater	1329:1381	This easily recoverable floating photocatalyst may be applied to improve the photocatalytic treatment of industrial wastewater, making most efficient use of photoactivation with no mechanical stirring.					
31279883	6	60	theme	UV	1075:1076	arg1	irradiation					1078:1088	UV irradiation	1075:1088	UV irradiation (125 W, 254 nm)	1075:1104	The photocatalytic activity under UV irradiation (125 W, 254 nm) was evaluated for tartrazine dye removal, achieving up to 89% discoloration upon the first use with TOC removal capacity even after seven use cycles.					
31279883	2	61	theme	TiO2	322:325	arg1	photocatalyst					342:354	the low-density TiO2 alginate-based photocatalyst	306:354	the low-density TiO2 alginate-based photocatalyst	306:354	In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH).					
31279883	1	62	theme	higher	215:220	arg1	efficiency					243:252	higher radiance utilization efficiency	215:252	higher radiance utilization efficiency	215:252	For the first time, TiO2 immobilized in alginate polymer was modified to turn it into a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation.					
31279883	0	63	theme	photocatalyst	71:83	arg1	optimization					16:27	optimization	16:27	optimization	16:27	Formulation and optimization of a novel TiO2/calcium alginate floating photocatalyst.					
31279883	0	63	theme	photocatalyst	71:83	arg1	Formulation					0:10	Formulation	0:10	Formulation	0:10	Formulation and optimization of a novel TiO2/calcium alginate floating photocatalyst.					
31279883	3	64	theme	central	608:614	arg1	design					636:641	a central composite rotatable design	606:641	a central composite rotatable design combined with the response surface methodology	606:688	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	2	65	dep	agent	415:419	arg1	CaCO3					422:426	CaCO3	422:426	CaCO3	422:426	In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH).					
31279883	2	65	dep	agent	415:419	arg1	NaHCO3					429:434	NaHCO3	429:434	NaHCO3	429:434	In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH).					
31279883	5	66	dep	size	872:875	arg1	0.9215 g/cm3					901:912	0.9215 g/cm3	901:912	0.9215 g/cm3	901:912	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	5	66	dep	size	872:875	arg1	density					892:898	density	892:898	density	892:898	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	5	66	dep	size	872:875	arg1	3.4 ± 0.4 mm					878:889	3.4 ± 0.4 mm	878:889	3.4 ± 0.4 mm	878:889	The resulting floating beads were uniform (size: 3.4 ± 0.4 mm, density: 0.9215 g/cm3), with a rough surface and porous internal structure, as observed in the characterizations using SEM/EDX, TGA, XRD, and FT-IR.					
31279883	1	67	theme	radiance	222:229	arg1	efficiency					243:252	higher radiance utilization efficiency	215:252	higher radiance utilization efficiency	215:252	For the first time, TiO2 immobilized in alginate polymer was modified to turn it into a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation.					
31279883	0	68	theme	floating	62:69	arg1	photocatalyst					71:83	a novel TiO2/calcium alginate floating photocatalyst	32:83	a novel TiO2/calcium alginate floating photocatalyst	32:83	Formulation and optimization of a novel TiO2/calcium alginate floating photocatalyst.					
31279883	3	69	theme	beads	567:571	arg1	%					553:553	100%	550:553	100% of floating beads	550:571	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	3	69	theme	beads	567:571	arg1	beads					567:571	floating beads	558:571	floating beads	558:571	The TiO2, CaCO3, and CH3COOH concentrations affect buoyancy, and the best response (100% of floating beads) after 24 h was determined using a central composite rotatable design combined with the response surface methodology.					
31279883	2	70	theme	ionotropic	375:384	arg1	gelation					386:393	ionotropic gelation	375:393	ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH)	375:463	In this work, the low-density TiO2 alginate-based photocatalyst was synthesized by ionotropic gelation using a gas-forming agent (CaCO3, NaHCO3) in an acid medium (CH3COOH).					
31279883	1	71	theme	utilization	231:241	arg1	efficiency					243:252	higher radiance utilization efficiency	215:252	higher radiance utilization efficiency	215:252	For the first time, TiO2 immobilized in alginate polymer was modified to turn it into a buoyant photocatalyst for achieving both higher radiance utilization efficiency and easier post-treatment separation.					
29995974	6	0	theme	FG	881:882	arg1	transporter					884:894	the ATP-binding cassette LptB2 FG transporter	850:894	the ATP-binding cassette LptB2 FG transporter	850:894	The most poorly understood step in LPS transport is how the ATP-binding cassette LptB2 FG transporter extracts LPS from the inner membrane.					
29995974	3	1	theme	strain	480:485	arg1	level					487:491	the species and strain level	464:491	level	487:491	Although the overall LPS structure is conserved, LPS molecules can differ in composition at the species and strain level.					
29995974	6	2	theme	ATP-binding	854:864	arg1	transporter					884:894	the ATP-binding cassette LptB2 FG transporter	850:894	the ATP-binding cassette LptB2 FG transporter	850:894	The most poorly understood step in LPS transport is how the ATP-binding cassette LptB2 FG transporter extracts LPS from the inner membrane.					
29995974	1	3	theme	many	245:248	arg1	antimicrobials					250:263	many antimicrobials	245:263	many antimicrobials	245:263	Most Gram-negative bacteria assemble lipopolysaccharides (LPS) on their surface to form a permeability barrier against many antimicrobials.					
29995974	0	4	theme	substrate	70:78	arg1	variants					80:87	substrate variants	70:87	substrate variants	70:87	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.					
29995974	0	5	from	cluster	2:8	arg1	exporter					48:55	the lipopolysaccharide exporter	25:55	the lipopolysaccharide exporter	25:55	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.					
29995974	9	6	theme	site	1357:1360	arg1	part					1340:1343	part	1340:1343	part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS	1340:1448	We also show this residue is part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS.					
29995974	6	7	theme	cassette	866:873	arg1	transporter					884:894	the ATP-binding cassette LptB2 FG transporter	850:894	the ATP-binding cassette LptB2 FG transporter	850:894	The most poorly understood step in LPS transport is how the ATP-binding cassette LptB2 FG transporter extracts LPS from the inner membrane.					
29995974	7	8	theme	transporter	1042:1052	arg1	cavity					1002:1007	the structural cavity	987:1007	the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS	987:1119	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	4	9	theme	inner	562:566	arg1	membrane					568:575	the inner membrane	558:575	the inner membrane	558:575	Some bacteria also regulate when to modify phosphates on LPS at the inner membrane in order to become resistant to cationic antimicrobial peptides.					
29995974	0	10	from	exporter	48:55	arg1	cluster					2:8	A cluster	0:8	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.	0:124	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.					
29995974	8	11	theme	LPS	1295:1297	arg1	phosphates					1299:1308	the LPS phosphates	1291:1308	the LPS phosphates	1291:1308	Alterations to this residue cause transport defects that are suppressed by the activation of the BasSR two-component signaling system, which results in modifications to the LPS phosphates.					
29995974	8	12	theme	BasSR	1219:1223	arg1	system					1249:1254	the BasSR two-component signaling system	1215:1254	the BasSR two-component signaling system	1215:1254	Alterations to this residue cause transport defects that are suppressed by the activation of the BasSR two-component signaling system, which results in modifications to the LPS phosphates.					
29995974	7	13	theme	LptB2	1033:1037	arg1	transporter					1042:1052	the Escherichia coli LptB2 FG transporter	1012:1052	the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS	1012:1119	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	3	14	theme	LPS	421:423	arg1	molecules					425:433	LPS molecules	421:433	LPS molecules	421:433	Although the overall LPS structure is conserved, LPS molecules can differ in composition at the species and strain level.					
29995974	0	15	theme	lipopolysaccharide	29:46	arg1	exporter					48:55	the lipopolysaccharide exporter	25:55	the lipopolysaccharide exporter	25:55	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.					
29995974	7	16	theme	FG	1039:1040	arg1	transporter					1042:1052	the Escherichia coli LptB2 FG transporter	1012:1052	the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS	1012:1119	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	6	17	from	step	821:824	arg1	transport					833:841	LPS transport	829:841	LPS transport	829:841	The most poorly understood step in LPS transport is how the ATP-binding cassette LptB2 FG transporter extracts LPS from the inner membrane.					
29995974	4	18	theme	antimicrobial	618:630	arg1	peptides					632:639	cationic antimicrobial peptides	609:639	cationic antimicrobial peptides	609:639	Some bacteria also regulate when to modify phosphates on LPS at the inner membrane in order to become resistant to cationic antimicrobial peptides.					
29995974	9	19	from	LptG	1365:1368	arg1	part					1340:1343	part	1340:1343	part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS	1340:1448	We also show this residue is part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS.					
29995974	4	20	from	membrane	568:575	arg1	phosphates					537:546	phosphates	537:546	phosphates on LPS at the inner membrane	537:575	Some bacteria also regulate when to modify phosphates on LPS at the inner membrane in order to become resistant to cationic antimicrobial peptides.					
29995974	7	21	theme	Escherichia	1016:1026	arg1	transporter					1042:1052	the Escherichia coli LptB2 FG transporter	1012:1052	the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS	1012:1119	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	9	22	theme	modified	1437:1444	arg1	LPS					1446:1448	unmodified and modified LPS	1422:1448	unmodified and modified LPS	1422:1448	We also show this residue is part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS.					
29995974	4	23	from	phosphates	537:546	arg1	LPS					551:553	LPS	551:553	LPS	551:553	Some bacteria also regulate when to modify phosphates on LPS at the inner membrane in order to become resistant to cationic antimicrobial peptides.					
29995974	9	24	theme	LPS	1446:1448	arg1	transport					1409:1417	the transport	1405:1417	the transport of unmodified and modified LPS	1405:1448	We also show this residue is part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS.					
29995974	6	25	theme	understood	810:819	arg1	step					821:824	The most poorly understood step	794:824	The most poorly understood step in LPS transport	794:841	The most poorly understood step in LPS transport is how the ATP-binding cassette LptB2 FG transporter extracts LPS from the inner membrane.					
29995974	2	26	theme	membrane	362:369	arg1	leaflet					341:347	the outer leaflet	331:347	the outer leaflet of the outer membrane	331:369	LPS is synthesized at the inner membrane and then transported to the outer leaflet of the outer membrane.					
29995974	0	27	theme	residues	13:20	arg1	cluster					2:8	A cluster	0:8	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.	0:124	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.					
29995974	6	28	from	membrane	924:931	arg1	LPS					905:907	LPS	905:907	LPS from the inner membrane	905:931	The most poorly understood step in LPS transport is how the ATP-binding cassette LptB2 FG transporter extracts LPS from the inner membrane.					
29995974	8	29	theme	transport	1156:1164	arg1	defects					1166:1172	transport defects	1156:1172	transport defects that are suppressed by the activation of the BasSR two-component signaling system, which results in modifications to the LPS phosphates	1156:1308	Alterations to this residue cause transport defects that are suppressed by the activation of the BasSR two-component signaling system, which results in modifications to the LPS phosphates.					
29995974	7	30	from	K34	958:960	arg1	LptG					965:968	LptG	965:968	LptG	965:968	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	0	31	theme	outer	110:114	arg1	membrane					116:123	the outer membrane	106:123	the outer membrane	106:123	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.					
29995974	5	32	from	inner	718:722	arg1	LPS					705:707	LPS	705:707	LPS from the inner to the outer membrane	705:744	The multi-protein Lpt trans-envelope machine, which transports LPS from the inner to the outer membrane, must therefore handle a variety of substrates.					
29995974	2	33	theme	inner	292:296	arg1	membrane					298:305	the inner membrane	288:305	the inner membrane	288:305	LPS is synthesized at the inner membrane and then transported to the outer leaflet of the outer membrane.					
29995974	2	34	theme	outer	356:360	arg1	membrane					362:369	the outer membrane	352:369	the outer membrane	352:369	LPS is synthesized at the inner membrane and then transported to the outer leaflet of the outer membrane.					
29995974	7	35	dep	Escherichia	1016:1026	arg1	coli					1028:1031	coli	1028:1031	coli	1028:1031	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	6	36	theme	inner	918:922	arg1	membrane					924:931	the inner membrane	914:931	the inner membrane	914:931	The most poorly understood step in LPS transport is how the ATP-binding cassette LptB2 FG transporter extracts LPS from the inner membrane.					
29995974	7	37	theme	structural	991:1000	arg1	cavity					1002:1007	the structural cavity	987:1007	the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS	987:1119	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	6	38	theme	LPS	829:831	arg1	transport					833:841	LPS transport	829:841	LPS transport	829:841	The most poorly understood step in LPS transport is how the ATP-binding cassette LptB2 FG transporter extracts LPS from the inner membrane.					
29995974	4	39	theme	cationic	609:616	arg1	peptides					632:639	cationic antimicrobial peptides	609:639	cationic antimicrobial peptides	609:639	Some bacteria also regulate when to modify phosphates on LPS at the inner membrane in order to become resistant to cationic antimicrobial peptides.					
29995974	7	40	theme	unmodified	1106:1115	arg1	LPS					1117:1119	unmodified LPS	1106:1119	unmodified LPS	1106:1119	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	9	41	from	site	1357:1360	arg1	LptG					1365:1368	LptG	1365:1368	LptG	1365:1368	We also show this residue is part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS.					
29995974	8	42	theme	signaling	1239:1247	arg1	system					1249:1254	the BasSR two-component signaling system	1215:1254	the BasSR two-component signaling system	1215:1254	Alterations to this residue cause transport defects that are suppressed by the activation of the BasSR two-component signaling system, which results in modifications to the LPS phosphates.					
29995974	5	43	theme	substrates	782:791	arg1	substrates					782:791	substrates	782:791	substrates	782:791	The multi-protein Lpt trans-envelope machine, which transports LPS from the inner to the outer membrane, must therefore handle a variety of substrates.					
29995974	5	43	theme	substrates	782:791	arg1	variety					771:777	a variety	769:777	a variety of substrates	769:791	The multi-protein Lpt trans-envelope machine, which transports LPS from the inner to the outer membrane, must therefore handle a variety of substrates.					
29995974	9	44	theme	larger	1350:1355	arg1	site					1357:1360	a larger site	1348:1360	a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS	1348:1448	We also show this residue is part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS.					
29995974	8	45	theme	two-component	1225:1237	arg1	system					1249:1254	the BasSR two-component signaling system	1215:1254	the BasSR two-component signaling system	1215:1254	Alterations to this residue cause transport defects that are suppressed by the activation of the BasSR two-component signaling system, which results in modifications to the LPS phosphates.					
29995974	3	46	theme	overall	385:391	arg1	structure					397:405	the overall LPS structure	381:405	the overall LPS structure	381:405	Although the overall LPS structure is conserved, LPS molecules can differ in composition at the species and strain level.					
29995974	5	47	theme	multi-protein	646:658	arg1	machine					679:685	The multi-protein Lpt trans-envelope machine	642:685	The multi-protein Lpt trans-envelope machine	642:685	The multi-protein Lpt trans-envelope machine, which transports LPS from the inner to the outer membrane, must therefore handle a variety of substrates.					
29995974	2	48	theme	outer	335:339	arg1	leaflet					341:347	the outer leaflet	331:347	the outer leaflet of the outer membrane	331:369	LPS is synthesized at the inner membrane and then transported to the outer leaflet of the outer membrane.					
29995974	3	49	theme	LPS	393:395	arg1	structure					397:405	the overall LPS structure	381:405	the overall LPS structure	381:405	Although the overall LPS structure is conserved, LPS molecules can differ in composition at the species and strain level.					
29995974	5	50	theme	Lpt	660:662	arg1	machine					679:685	The multi-protein Lpt trans-envelope machine	642:685	The multi-protein Lpt trans-envelope machine	642:685	The multi-protein Lpt trans-envelope machine, which transports LPS from the inner to the outer membrane, must therefore handle a variety of substrates.					
29995974	6	51	theme	LptB2	875:879	arg1	transporter					884:894	the ATP-binding cassette LptB2 FG transporter	850:894	the ATP-binding cassette LptB2 FG transporter	850:894	The most poorly understood step in LPS transport is how the ATP-binding cassette LptB2 FG transporter extracts LPS from the inner membrane.					
29995974	8	52	theme	system	1249:1254	arg1	activation					1201:1210	the activation	1197:1210	the activation	1197:1210	Alterations to this residue cause transport defects that are suppressed by the activation of the BasSR two-component signaling system, which results in modifications to the LPS phosphates.					
29995974	5	53	theme	trans-envelope	664:677	arg1	machine					679:685	The multi-protein Lpt trans-envelope machine	642:685	The multi-protein Lpt trans-envelope machine	642:685	The multi-protein Lpt trans-envelope machine, which transports LPS from the inner to the outer membrane, must therefore handle a variety of substrates.					
29995974	7	54	from	phosphates	1092:1101	arg1	LPS					1117:1119	unmodified LPS	1106:1119	unmodified LPS	1106:1119	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	9	55	from	part	1340:1343	arg1	LptG					1365:1368	LptG	1365:1368	LptG	1365:1368	We also show this residue is part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS.					
29995974	1	56	theme	Most	126:129	arg1	bacteria					145:152	Most Gram-negative bacteria	126:152	Most Gram-negative bacteria	126:152	Most Gram-negative bacteria assemble lipopolysaccharides (LPS) on their surface to form a permeability barrier against many antimicrobials.					
29995974	1	57	theme	permeability	216:227	arg1	barrier					229:235	a permeability barrier	214:235	a permeability barrier against many antimicrobials	214:263	Most Gram-negative bacteria assemble lipopolysaccharides (LPS) on their surface to form a permeability barrier against many antimicrobials.					
29995974	9	58	theme	unmodified	1422:1431	arg1	LPS					1446:1448	unmodified and modified LPS	1422:1448	unmodified and modified LPS	1422:1448	We also show this residue is part of a larger site in LptG that differentially contributes to the transport of unmodified and modified LPS.					
29995974	7	59	theme	residue	950:956	arg1	site					975:978	a site	973:978	a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS	973:1119	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	7	59	theme	residue	950:956	arg1	K34					958:960	residue K34	950:960	residue K34 in LptG	950:968	Here, we define residue K34 in LptG as a site within the structural cavity of the Escherichia coli LptB2 FG transporter that interacts electrostatically with phosphates on unmodified LPS.					
29995974	1	60	theme	Gram-negative	131:143	arg1	bacteria					145:152	Most Gram-negative bacteria	126:152	Most Gram-negative bacteria	126:152	Most Gram-negative bacteria assemble lipopolysaccharides (LPS) on their surface to form a permeability barrier against many antimicrobials.					
29995974	5	61	theme	outer	731:735	arg1	membrane					737:744	the outer membrane	727:744	the outer membrane	727:744	The multi-protein Lpt trans-envelope machine, which transports LPS from the inner to the outer membrane, must therefore handle a variety of substrates.					
29995974	0	62	from	residues	13:20	arg1	exporter					48:55	the lipopolysaccharide exporter	25:55	the lipopolysaccharide exporter	25:55	A cluster of residues in the lipopolysaccharide exporter that selects substrate variants for transport to the outer membrane.					
30663787	0	0	theme	selector	107:114	arg1	systems					116:122	dual selector systems	102:122	dual selector systems	102:122	Investigation of the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid based dual selector systems toward basic drugs in capillary electrophoresis.					
30663787	3	1	with	system	612:617	arg1	CP					647:648	CP	647:648	CP	647:648	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	3	1	with	system	612:617	arg1	phosphate					636:644	clindamycin phosphate	624:644	clindamycin phosphate (CP)	624:649	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	4	2	theme	single	692:697	arg1	system					702:707	single CP system	692:707	single CP system	692:707	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	1	3	theme	chiral	183:188	arg1	CILs					205:208	CILs	205:208	CILs	205:208	Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by CE.					
30663787	1	3	theme	chiral	183:188	arg1	liquids					196:202	chiral ionic liquids	183:202	chiral ionic liquids (CILs)	183:209	Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by CE.					
30663787	0	4	theme	dual	102:105	arg1	systems					116:122	dual selector systems	102:122	dual selector systems	102:122	Investigation of the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid based dual selector systems toward basic drugs in capillary electrophoresis.					
30663787	5	5	theme	TMA-LA/CP	1054:1062	arg1	concentration					1037:1049	concentration	1037:1049	concentration	1037:1049	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	5	theme	TMA-LA/CP	1054:1062	arg1	pH					1029:1030	pH	1029:1030	pH	1029:1030	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	5	theme	TMA-LA/CP	1054:1062	arg1	modifier					996:1003	organic modifier	988:1003	organic modifier	988:1003	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	5	theme	TMA-LA/CP	1054:1062	arg1	composition					1013:1023	buffer composition	1006:1023	buffer composition	1006:1023	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	6	6	theme	Autodock	1313:1320	arg1	software					1304:1311	the molecular docking software Autodock	1282:1320	the molecular docking software Autodock	1282:1320	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	1	7	theme	ionic	190:194	arg1	CILs					205:208	CILs	205:208	CILs	205:208	Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by CE.					
30663787	1	7	theme	ionic	190:194	arg1	liquids					196:202	chiral ionic liquids	183:202	chiral ionic liquids (CILs)	183:209	Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by CE.					
30663787	7	8	theme	separation	1513:1522	arg1	reason					1494:1499	the reason	1490:1499	the reason of improved separation in TMA-LA/CP system	1490:1542	The existence of TMA-LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA-LA/CP system.					
30663787	6	9	theme	TMA-LA/CP	1234:1242	arg1	system					1269:1274	the TMA-LA/CP dual-selector separation system	1230:1274	the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results	1230:1367	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	2	10	theme	deep	299:302	arg1	perception					304:313	deep perception	299:313	deep perception of the specific mechanism about CILs in CE for enantioseparation	299:378	Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts.					
30663787	5	11	theme	crucial	938:944	arg1	proportion					974:983	type and proportion	965:983	proportion	974:983	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	11	theme	crucial	938:944	arg1	parameters					946:955	Several crucial parameters	930:955	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP	930:1062	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	11	theme	crucial	938:944	arg1	type					965:968	type and proportion	965:983	type	965:968	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	3	12	theme	first	568:572	arg1	time					574:577	the first time	564:577	the first time	564:577	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	6	13	theme	docking	1296:1302	arg1	software					1304:1311	the molecular docking software Autodock	1282:1320	the molecular docking software Autodock	1282:1320	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	3	14	theme	liquid	498:503	arg1	TMA-LA					540:545	TMA-LA	540:545	TMA-LA	540:545	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	3	14	theme	liquid	498:503	arg1	tetramethylammonium-lactobionate					506:537	tetramethylammonium-lactobionate	506:537	tetramethylammonium-lactobionate (TMA-LA)	506:546	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	7	15	theme	discriminatory	1431:1444	arg1	ability					1446:1452	a higher discriminatory ability	1422:1452	a higher discriminatory ability against the enantiomers	1422:1476	The existence of TMA-LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA-LA/CP system.					
30663787	8	16	theme	influence	1553:1561	arg1	factors					1563:1569	All the influence factors	1545:1569	All the influence factors	1545:1569	All the influence factors evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the chiral separation system.					
30663787	6	17	theme	molecular	1286:1294	arg1	software					1304:1311	the molecular docking software Autodock	1282:1320	the molecular docking software Autodock	1282:1320	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	2	18	from	CILs	347:350	arg1	CE					355:356	CE	355:356	CE	355:356	Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts.					
30663787	4	19	theme	tested	777:782	arg1	propranolol					799:809	propranolol	799:809	propranolol	799:809	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	19	theme	tested	777:782	arg1	chlorphenamine					833:846	chlorphenamine	833:846	chlorphenamine	833:846	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	19	theme	tested	777:782	arg1	esmolol					877:883	esmolol	877:883	esmolol	877:883	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	19	theme	tested	777:782	arg1	metoprolol					849:858	metoprolol	849:858	metoprolol	849:858	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	19	theme	tested	777:782	arg1	nefopam					812:818	nefopam	812:818	nefopam	812:818	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	19	theme	tested	777:782	arg1	citalopram					821:830	citalopram	821:830	citalopram	821:830	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	19	theme	tested	777:782	arg1	bisoprolol					861:870	bisoprolol	861:870	bisoprolol	861:870	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	19	theme	tested	777:782	arg1	drugs					792:796	seven tested rameric drugs	771:796	seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol)	771:884	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	0	20	theme	basic	131:135	arg1	drugs					137:141	basic drugs	131:141	basic drugs in capillary electrophoresis	131:170	Investigation of the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid based dual selector systems toward basic drugs in capillary electrophoresis.					
30663787	6	21	theme	molecular	1148:1156	arg1	modeling					1158:1165	molecular modeling	1148:1165	molecular modeling	1148:1165	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	8	22	theme	Statistical	1593:1603	arg1	Product					1605:1611	Statistical Product	1593:1611	Statistical Product	1593:1611	All the influence factors evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the chiral separation system.					
30663787	2	23	theme	specific	322:329	arg1	mechanism					331:339	the specific mechanism	318:339	the specific mechanism	318:339	Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts.					
30663787	4	24	dep	drugs	792:796	arg1	propranolol					799:809	propranolol	799:809	propranolol	799:809	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	24	dep	drugs	792:796	arg1	chlorphenamine					833:846	chlorphenamine	833:846	chlorphenamine	833:846	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	24	dep	drugs	792:796	arg1	esmolol					877:883	esmolol	877:883	esmolol	877:883	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	24	dep	drugs	792:796	arg1	metoprolol					849:858	metoprolol	849:858	metoprolol	849:858	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	24	dep	drugs	792:796	arg1	nefopam					812:818	nefopam	812:818	nefopam	812:818	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	24	dep	drugs	792:796	arg1	citalopram					821:830	citalopram	821:830	citalopram	821:830	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	24	dep	drugs	792:796	arg1	bisoprolol					861:870	bisoprolol	861:870	bisoprolol	861:870	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	24	dep	drugs	792:796	arg1	drugs					792:796	seven tested rameric drugs	771:796	seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol)	771:884	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	6	25	theme	recognition	1205:1215	arg1	mechanism					1217:1225	the chiral recognition mechanism	1194:1225	the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results	1194:1367	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	6	26	theme	system	1269:1274	arg1	mechanism					1217:1225	the chiral recognition mechanism	1194:1225	the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results	1194:1367	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	7	27	theme	TMA-LA/CP	1527:1535	arg1	system					1537:1542	TMA-LA/CP system	1527:1542	TMA-LA/CP system	1527:1542	The existence of TMA-LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA-LA/CP system.					
30663787	3	28	theme	enantiomeric	655:666	arg1	separation					668:677	enantiomeric separation	655:677	enantiomeric separation	655:677	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	3	29	dep	establish	588:596	arg1	CE					582:583	CE	582:583	CE	582:583	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	0	30	theme	enantioselectivity	21:38	arg1	Investigation					0:12	Investigation	0:12	Investigation of the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid	0:94	Investigation of the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid based dual selector systems toward basic drugs in capillary electrophoresis.					
30663787	4	31	theme	TMA-LA	719:724	arg1	system					726:731	single TMA-LA system	712:731	single TMA-LA system	712:731	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	0	32	theme	capillary	146:154	arg1	electrophoresis					156:170	capillary electrophoresis	146:170	capillary electrophoresis	146:170	Investigation of the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid based dual selector systems toward basic drugs in capillary electrophoresis.					
30663787	7	33	from	reason	1494:1499	arg1	system					1537:1542	TMA-LA/CP system	1527:1542	TMA-LA/CP system	1527:1542	The existence of TMA-LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA-LA/CP system.					
30663787	4	34	theme	improved	747:754	arg1	separations					756:766	significantly improved separations	733:766	significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol)	733:884	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	6	35	theme	separation	1258:1267	arg1	system					1269:1274	the TMA-LA/CP dual-selector separation system	1230:1274	the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results	1230:1367	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	2	36	theme	strenuous	433:441	arg1	efforts					443:449	the strenuous efforts	429:449	the strenuous efforts	429:449	Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts.					
30663787	6	37	theme	chiral	1198:1203	arg1	mechanism					1217:1225	the chiral recognition mechanism	1194:1225	the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results	1194:1367	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	2	38	theme	perception	304:313	arg1	lack					291:294	the lack	287:294	the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation	287:378	Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts.					
30663787	7	39	theme	complex	1397:1403	arg1	existence					1374:1382	The existence	1370:1382	The existence of TMA-LA/CP complex	1370:1403	The existence of TMA-LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA-LA/CP system.					
30663787	4	40	theme	CP	699:700	arg1	system					702:707	single CP system	692:707	single CP system	692:707	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	41	theme	selector	912:919	arg1	systems					921:927	the dual selector systems	903:927	the dual selector systems	903:927	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	5	42	theme	modifier	996:1003	arg1	proportion					974:983	type and proportion	965:983	proportion	974:983	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	42	theme	modifier	996:1003	arg1	type					965:968	type and proportion	965:983	type	965:968	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	4	43	located	observed	891:898	arg1	systems					921:927	the dual selector systems	903:927	the dual selector systems	903:927	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	43	located	observed	891:898	arg2	separations					756:766	significantly improved separations	733:766	significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol)	733:884	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	1	44	theme	increasing	225:234	arg1	interest					236:243	increasing interest	225:243	increasing interest in chiral separation by CE	225:270	Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by CE.					
30663787	8	45	theme	separation	1683:1692	arg1	system					1694:1699	the chiral separation system	1672:1699	the chiral separation system	1672:1699	All the influence factors evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the chiral separation system.					
30663787	7	46	theme	improved	1504:1511	arg1	separation					1513:1522	improved separation	1504:1522	improved separation	1504:1522	The existence of TMA-LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA-LA/CP system.					
30663787	4	47	theme	dual	907:910	arg1	systems					921:927	the dual selector systems	903:927	the dual selector systems	903:927	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	3	48	theme	clindamycin	624:634	arg1	CP					647:648	CP	647:648	CP	647:648	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	3	48	theme	clindamycin	624:634	arg1	phosphate					636:644	clindamycin phosphate	624:644	clindamycin phosphate (CP)	624:649	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	6	49	theme	dual-selector	1244:1256	arg1	system					1269:1274	the TMA-LA/CP dual-selector separation system	1230:1274	the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results	1230:1367	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	2	50	theme	researchers	408:418	arg1	legion					398:403	a legion	396:403	a legion of researchers	396:418	Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts.					
30663787	8	51	theme	Service	1617:1623	arg1	SPSS					1636:1639	SPSS	1636:1639	SPSS	1636:1639	All the influence factors evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the chiral separation system.					
30663787	8	51	theme	Service	1617:1623	arg1	Solutions					1625:1633	Service Solutions	1617:1633	Service Solutions (SPSS)	1617:1640	All the influence factors evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the chiral separation system.					
30663787	7	52	theme	TMA-LA/CP	1387:1395	arg1	complex					1397:1403	TMA-LA/CP complex	1387:1403	TMA-LA/CP complex	1387:1403	The existence of TMA-LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA-LA/CP system.					
30663787	5	53	theme	buffer	1006:1011	arg1	composition					1013:1023	buffer composition	1006:1023	buffer composition	1006:1023	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	1	54	from	interest	236:243	arg1	separation					255:264	chiral separation	248:264	chiral separation by CE	248:270	Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by CE.					
30663787	6	55	theme	experimental	1348:1359	arg1	results					1361:1367	the experimental results	1344:1367	the experimental results	1344:1367	Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA-LA/CP dual-selector separation system using the molecular docking software Autodock, which well supported the experimental results.					
30663787	0	56	from	drugs	137:141	arg1	electrophoresis					156:170	capillary electrophoresis	146:170	capillary electrophoresis	146:170	Investigation of the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid based dual selector systems toward basic drugs in capillary electrophoresis.					
30663787	5	57	theme	composition	1013:1023	arg1	proportion					974:983	type and proportion	965:983	proportion	974:983	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	57	theme	composition	1013:1023	arg1	type					965:968	type and proportion	965:983	type	965:968	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	3	58	theme	ionic	492:496	arg1	TMA-LA					540:545	TMA-LA	540:545	TMA-LA	540:545	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	3	58	theme	ionic	492:496	arg1	tetramethylammonium-lactobionate					506:537	tetramethylammonium-lactobionate	506:537	tetramethylammonium-lactobionate (TMA-LA)	506:546	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	4	59	theme	single	712:717	arg1	system					726:731	single TMA-LA system	712:731	single TMA-LA system	712:731	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	5	60	theme	organic	988:994	arg1	modifier					996:1003	organic modifier	988:1003	organic modifier	988:1003	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	8	61	from	influences	1658:1667	arg1	system					1694:1699	the chiral separation system	1672:1699	the chiral separation system	1672:1699	All the influence factors evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the chiral separation system.					
30663787	5	62	theme	pH	1029:1030	arg1	proportion					974:983	type and proportion	965:983	proportion	974:983	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	62	theme	pH	1029:1030	arg1	type					965:968	type and proportion	965:983	type	965:968	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	4	63	theme	drugs	792:796	arg1	separations					756:766	significantly improved separations	733:766	significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol)	733:884	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	3	64	theme	lactobionic	469:479	arg1	acid					481:484	a lactobionic acid	467:484	a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA)	467:546	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	5	65	theme	Several	930:936	arg1	proportion					974:983	type and proportion	965:983	proportion	974:983	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	65	theme	Several	930:936	arg1	parameters					946:955	Several crucial parameters	930:955	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP	930:1062	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	5	65	theme	Several	930:936	arg1	type					965:968	type and proportion	965:983	type	965:968	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	7	66	theme	higher	1424:1429	arg1	ability					1446:1452	a higher discriminatory ability	1422:1452	a higher discriminatory ability against the enantiomers	1422:1476	The existence of TMA-LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA-LA/CP system.					
30663787	4	67	theme	rameric	784:790	arg1	propranolol					799:809	propranolol	799:809	propranolol	799:809	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	67	theme	rameric	784:790	arg1	chlorphenamine					833:846	chlorphenamine	833:846	chlorphenamine	833:846	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	67	theme	rameric	784:790	arg1	esmolol					877:883	esmolol	877:883	esmolol	877:883	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	67	theme	rameric	784:790	arg1	metoprolol					849:858	metoprolol	849:858	metoprolol	849:858	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	67	theme	rameric	784:790	arg1	nefopam					812:818	nefopam	812:818	nefopam	812:818	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	67	theme	rameric	784:790	arg1	citalopram					821:830	citalopram	821:830	citalopram	821:830	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	67	theme	rameric	784:790	arg1	bisoprolol					861:870	bisoprolol	861:870	bisoprolol	861:870	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	4	67	theme	rameric	784:790	arg1	drugs					792:796	seven tested rameric drugs	771:796	seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol)	771:884	Compared to single CP system or single TMA-LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems.					
30663787	2	68	theme	mechanism	331:339	arg1	perception					304:313	deep perception	299:313	deep perception of the specific mechanism about CILs in CE for enantioseparation	299:378	Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts.					
30663787	1	69	theme	chiral	248:253	arg1	separation					255:264	chiral separation	248:264	chiral separation by CE	248:270	Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by CE.					
30663787	3	70	theme	dual	598:601	arg1	system					612:617	dual selector system	598:617	dual selector system with clindamycin phosphate (CP) for enantiomeric separation	598:677	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
30663787	8	71	theme	chiral	1676:1681	arg1	system					1694:1699	the chiral separation system	1672:1699	the chiral separation system	1672:1699	All the influence factors evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the chiral separation system.					
30663787	0	72	theme	liquid	89:94	arg1	enantioselectivity					21:38	the enantioselectivity	17:38	the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid	17:94	Investigation of the enantioselectivity of tetramethylammonium-lactobionate chiral ionic liquid based dual selector systems toward basic drugs in capillary electrophoresis.					
30663787	5	73	theme	satisfied	1108:1116	arg1	enantioseparation					1118:1134	satisfied enantioseparation	1108:1134	satisfied enantioseparation	1108:1134	Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA-LA/CP were systematically investigated to achieve satisfied enantioseparation.					
30663787	3	74	theme	selector	603:610	arg1	system					612:617	dual selector system	598:617	dual selector system with clindamycin phosphate (CP) for enantiomeric separation	598:677	In this paper, a lactobionic acid based ionic liquid, tetramethylammonium-lactobionate (TMA-LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation.					
31128711	9	0	from	genes	1208:1212	arg1	maize					1217:1221	maize	1217:1221	maize	1217:1221	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	8	1	theme	key	991:993	arg1	genes					1009:1013	eight key starch enzyme genes	985:1013	eight key starch enzyme genes	985:1013	Screening by yeast one-hybrid assays indicated a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes.					
31128711	6	2	theme	ZmbZIP22	668:675	arg1	overexpression					650:663	overexpression	650:663	overexpression of ZmbZIP22	650:675	Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize.					
31128711	6	3	from	increases	689:697	arg1	contents					706:713	the contents	702:713	the contents of soluble sugars and reducing sugars in transgenic rice and maize	702:780	Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize.					
31128711	5	4	theme	seeds	550:554	arg1	weight					540:545	weight	540:545	weight	540:545	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	4	theme	seeds	550:554	arg1	content					573:579	reduced amylose content	557:579	reduced amylose content	557:579	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	4	theme	seeds	550:554	arg1	alterations					586:596	alterations	586:596	alterations in the chemical structure of starch granules	586:641	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	4	theme	seeds	550:554	arg1	size					531:534	size	531:534	size	531:534	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	4	theme	seeds	550:554	arg1	reductions					484:493	reductions	484:493	reductions in the size of starch granules	484:524	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	1	5	theme	yield	156:160	arg1	composition					118:128	composition	118:128	composition	118:128	Starch content and composition are major determinants of yield and quality in maize.					
31128711	1	5	theme	yield	156:160	arg1	determinants					140:151	major determinants	134:151	major determinants of yield and quality in maize	134:181	Starch content and composition are major determinants of yield and quality in maize.					
31128711	1	5	theme	yield	156:160	arg1	content					106:112	Starch content	99:112	Starch content	99:112	Starch content and composition are major determinants of yield and quality in maize.					
31128711	5	6	dep	size	531:534	arg1	the					527:529	the	527:529	the	527:529	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	7	theme	chemical	605:612	arg1	structure					614:622	the chemical structure	601:622	the chemical structure of starch granules	601:641	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	8	theme	reduced	557:563	arg1	content					573:579	reduced amylose content	557:579	reduced amylose content	557:579	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	7	9	theme	metabolism	829:838	arg1	genes					840:844	starch metabolism genes	822:844	starch metabolism genes	822:844	ZmbZIP22 promotes the transcription of starch metabolism genes by binding to their promoters.					
31128711	0	10	from	content	56:62	arg1	rice					93:96	rice	93:96	rice	93:96	Overexpression of ZmbZIP22 gene alters endosperm starch content and composition in maize and rice.					
31128711	0	10	from	content	56:62	arg1	maize					83:87	maize	83:87	maize	83:87	Overexpression of ZmbZIP22 gene alters endosperm starch content and composition in maize and rice.					
31128711	8	11	theme	starch	995:1000	arg1	genes					1009:1013	eight key starch enzyme genes	985:1013	eight key starch enzyme genes	985:1013	Screening by yeast one-hybrid assays indicated a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes.					
31128711	4	12	theme	ZmbZIP22	381:388	arg1	factor					424:429	a bZIP transcription factor	403:429	a bZIP transcription factor	403:429	The ZmbZIP22 gene encodes a bZIP transcription factor.					
31128711	4	12	theme	ZmbZIP22	381:388	arg1	gene					390:393	The ZmbZIP22 gene	377:393	The ZmbZIP22 gene	377:393	The ZmbZIP22 gene encodes a bZIP transcription factor.					
31128711	9	13	theme	key	1176:1178	arg1	sugar					1180:1184	key sugar and starch metabolism genes	1176:1212	sugar	1180:1184	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	9	14	from	regulation	1162:1171	arg1	maize					1217:1221	maize	1217:1221	maize	1217:1221	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	3	15	theme	factors	308:314	arg1	role					286:289	the role	282:289	the role of transcription factors in regulating the starch metabolism pathway	282:358	However, the role of transcription factors in regulating the starch metabolism pathway remains unclear.					
31128711	7	16	theme	starch	822:827	arg1	genes					840:844	starch metabolism genes	822:844	starch metabolism genes	822:844	ZmbZIP22 promotes the transcription of starch metabolism genes by binding to their promoters.					
31128711	1	17	from	determinants	140:151	arg1	maize					177:181	maize	177:181	maize	177:181	Starch content and composition are major determinants of yield and quality in maize.					
31128711	8	18	theme	genes	1009:1013	arg1	ZmbZIP22					955:962	ZmbZIP22	955:962	ZmbZIP22	955:962	Screening by yeast one-hybrid assays indicated a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes.					
31128711	8	18	theme	genes	1009:1013	arg1	promoters					972:980	the promoters	968:980	the promoters of eight key starch enzyme genes	968:1013	Screening by yeast one-hybrid assays indicated a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes.					
31128711	6	19	theme	reducing	737:744	arg1	sugars					746:751	reducing sugars	737:751	reducing sugars	737:751	Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize.					
31128711	9	20	theme	starch	1190:1195	arg1	genes					1208:1212	key sugar and starch metabolism genes	1176:1212	genes	1208:1212	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	1	21	theme	quality	166:172	arg1	composition					118:128	composition	118:128	composition	118:128	Starch content and composition are major determinants of yield and quality in maize.					
31128711	1	21	theme	quality	166:172	arg1	determinants					140:151	major determinants	134:151	major determinants of yield and quality in maize	134:181	Starch content and composition are major determinants of yield and quality in maize.					
31128711	1	21	theme	quality	166:172	arg1	content					106:112	Starch content	99:112	Starch content	99:112	Starch content and composition are major determinants of yield and quality in maize.					
31128711	0	22	theme	gene	27:30	arg1	Overexpression					0:13	Overexpression	0:13	Overexpression of ZmbZIP22 gene	0:30	Overexpression of ZmbZIP22 gene alters endosperm starch content and composition in maize and rice.					
31128711	6	23	theme	sugars	726:731	arg1	contents					706:713	the contents	702:713	the contents of soluble sugars and reducing sugars in transgenic rice and maize	702:780	Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize.					
31128711	3	24	theme	starch	334:339	arg1	pathway					352:358	the starch metabolism pathway	330:358	the starch metabolism pathway	330:358	However, the role of transcription factors in regulating the starch metabolism pathway remains unclear.					
31128711	5	25	from	alterations	586:596	arg1	size					502:505	the size	498:505	the size of starch granules	498:524	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	25	from	alterations	586:596	arg1	structure					614:622	the chemical structure	601:622	the chemical structure of starch granules	601:641	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	26	theme	amylose	565:571	arg1	content					573:579	reduced amylose content	557:579	reduced amylose content	557:579	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	9	27	theme	negative	1081:1088	arg1	regulator					1090:1098	a negative regulator	1079:1098	a negative regulator of starch synthesis	1079:1118	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	9	27	theme	negative	1081:1088	arg1	ZmbZIP22					1057:1064	ZmbZIP22	1057:1064	ZmbZIP22	1057:1064	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	3	28	theme	metabolism	341:350	arg1	pathway					352:358	the starch metabolism pathway	330:358	the starch metabolism pathway	330:358	However, the role of transcription factors in regulating the starch metabolism pathway remains unclear.					
31128711	5	29	from	content	573:579	arg1	size					502:505	the size	498:505	the size of starch granules	498:524	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	29	from	content	573:579	arg1	structure					614:622	the chemical structure	601:622	the chemical structure of starch granules	601:641	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	9	30	theme	sugar	1180:1184	arg1	regulation					1162:1171	the regulation	1158:1171	the regulation of key sugar and starch metabolism genes in maize	1158:1221	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	1	31	theme	Starch	99:104	arg1	composition					118:128	composition	118:128	composition	118:128	Starch content and composition are major determinants of yield and quality in maize.					
31128711	1	31	theme	Starch	99:104	arg1	determinants					140:151	major determinants	134:151	major determinants of yield and quality in maize	134:181	Starch content and composition are major determinants of yield and quality in maize.					
31128711	1	31	theme	Starch	99:104	arg1	content					106:112	Starch content	99:112	Starch content	99:112	Starch content and composition are major determinants of yield and quality in maize.					
31128711	0	32	theme	endosperm	39:47	arg1	content					56:62	endosperm starch content	39:62	endosperm starch content	39:62	Overexpression of ZmbZIP22 gene alters endosperm starch content and composition in maize and rice.					
31128711	4	33	theme	transcription	410:422	arg1	factor					424:429	a bZIP transcription factor	403:429	a bZIP transcription factor	403:429	The ZmbZIP22 gene encodes a bZIP transcription factor.					
31128711	4	33	theme	transcription	410:422	arg1	gene					390:393	The ZmbZIP22 gene	377:393	The ZmbZIP22 gene	377:393	The ZmbZIP22 gene encodes a bZIP transcription factor.					
31128711	0	34	from	composition	68:78	arg1	rice					93:96	rice	93:96	rice	93:96	Overexpression of ZmbZIP22 gene alters endosperm starch content and composition in maize and rice.					
31128711	0	34	from	composition	68:78	arg1	maize					83:87	maize	83:87	maize	83:87	Overexpression of ZmbZIP22 gene alters endosperm starch content and composition in maize and rice.					
31128711	2	35	theme	major	205:209	arg1	genes					211:215	the major genes	201:215	the major genes for starch metabolism	201:237	In recent years, the major genes for starch metabolism have been cloned in this species.					
31128711	5	36	theme	starch	510:515	arg1	granules					517:524	starch granules	510:524	starch granules	510:524	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	8	37	theme	possible	926:933	arg1	interaction					935:945	a possible interaction	924:945	a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes	924:1013	Screening by yeast one-hybrid assays indicated a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes.					
31128711	9	38	theme	metabolism	1197:1206	arg1	genes					1208:1212	key sugar and starch metabolism genes	1176:1212	genes	1208:1212	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	9	39	from	sugar	1180:1184	arg1	maize					1217:1221	maize	1217:1221	maize	1217:1221	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	5	40	theme	granules	517:524	arg1	size					502:505	the size	498:505	the size of starch granules	498:524	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	41	from	weight	540:545	arg1	size					502:505	the size	498:505	the size of starch granules	498:524	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	41	from	weight	540:545	arg1	structure					614:622	the chemical structure	601:622	the chemical structure of starch granules	601:641	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	0	42	theme	starch	49:54	arg1	content					56:62	endosperm starch content	39:62	endosperm starch content	39:62	Overexpression of ZmbZIP22 gene alters endosperm starch content and composition in maize and rice.					
31128711	5	43	theme	starch	627:632	arg1	granules					634:641	starch granules	627:641	starch granules	627:641	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	9	44	theme	genes	1208:1212	arg1	regulation					1162:1171	the regulation	1158:1171	the regulation of key sugar and starch metabolism genes in maize	1158:1221	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	8	45	theme	yeast	890:894	arg1	assays					907:912	yeast one-hybrid assays	890:912	yeast one-hybrid assays	890:912	Screening by yeast one-hybrid assays indicated a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes.					
31128711	8	46	theme	enzyme	1002:1007	arg1	genes					1009:1013	eight key starch enzyme genes	985:1013	eight key starch enzyme genes	985:1013	Screening by yeast one-hybrid assays indicated a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes.					
31128711	6	47	theme	soluble	718:724	arg1	sugars					726:731	soluble sugars	718:731	soluble sugars	718:731	Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize.					
31128711	5	48	theme	granules	634:641	arg1	structure					614:622	the chemical structure	601:622	the chemical structure of starch granules	601:641	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	2	49	theme	recent	187:192	arg1	years					194:198	recent years	187:198	recent years	187:198	In recent years, the major genes for starch metabolism have been cloned in this species.					
31128711	6	50	theme	transgenic	756:765	arg1	rice					767:770	transgenic rice	756:770	transgenic rice	756:770	Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize.					
31128711	5	51	from	size	531:534	arg1	size					502:505	the size	498:505	the size of starch granules	498:524	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	51	from	size	531:534	arg1	structure					614:622	the chemical structure	601:622	the chemical structure of starch granules	601:641	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	9	52	theme	starch	1103:1108	arg1	synthesis					1110:1118	starch synthesis	1103:1118	starch synthesis	1103:1118	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	4	53	theme	bZIP	405:408	arg1	factor					424:429	a bZIP transcription factor	403:429	a bZIP transcription factor	403:429	The ZmbZIP22 gene encodes a bZIP transcription factor.					
31128711	4	53	theme	bZIP	405:408	arg1	gene					390:393	The ZmbZIP22 gene	377:393	The ZmbZIP22 gene	377:393	The ZmbZIP22 gene encodes a bZIP transcription factor.					
31128711	5	54	from	reductions	484:493	arg1	size					502:505	the size	498:505	the size of starch granules	498:524	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	5	54	from	reductions	484:493	arg1	structure					614:622	the chemical structure	601:622	the chemical structure of starch granules	601:641	In our study, plants overexpressing ZmbZIP22 showed reductions in the size of starch granules, the size and weight of seeds, reduced amylose content, and alterations in the chemical structure of starch granules.					
31128711	9	55	theme	synthesis	1110:1118	arg1	regulator					1090:1098	a negative regulator	1079:1098	a negative regulator of starch synthesis	1079:1118	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	9	55	theme	synthesis	1110:1118	arg1	ZmbZIP22					1057:1064	ZmbZIP22	1057:1064	ZmbZIP22	1057:1064	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	6	56	from	contents	706:713	arg1	maize					776:780	maize	776:780	maize	776:780	Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize.					
31128711	6	56	from	contents	706:713	arg1	rice					767:770	transgenic rice	756:770	transgenic rice	756:770	Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize.					
31128711	6	57	theme	sugars	746:751	arg1	contents					706:713	the contents	702:713	the contents of soluble sugars and reducing sugars in transgenic rice and maize	702:780	Also, overexpression of ZmbZIP22 resulted in increases in the contents of soluble sugars and reducing sugars in transgenic rice and maize.					
31128711	9	58	from	maize	1217:1221	arg1	regulation					1162:1171	the regulation	1158:1171	the regulation of key sugar and starch metabolism genes in maize	1158:1221	Collectively, our results indicated that ZmbZIP22 functions as a negative regulator of starch synthesis, and suggest that this occurs through the regulation of key sugar and starch metabolism genes in maize.					
31128711	2	59	theme	starch	221:226	arg1	metabolism					228:237	starch metabolism	221:237	starch metabolism	221:237	In recent years, the major genes for starch metabolism have been cloned in this species.					
31128711	8	60	theme	one-hybrid	896:905	arg1	assays					907:912	yeast one-hybrid assays	890:912	yeast one-hybrid assays	890:912	Screening by yeast one-hybrid assays indicated a possible interaction between ZmbZIP22 and the promoters of eight key starch enzyme genes.					
31128711	1	61	theme	major	134:138	arg1	composition					118:128	composition	118:128	composition	118:128	Starch content and composition are major determinants of yield and quality in maize.					
31128711	1	61	theme	major	134:138	arg1	determinants					140:151	major determinants	134:151	major determinants of yield and quality in maize	134:181	Starch content and composition are major determinants of yield and quality in maize.					
31128711	1	61	theme	major	134:138	arg1	content					106:112	Starch content	99:112	Starch content	99:112	Starch content and composition are major determinants of yield and quality in maize.					
31128711	3	62	theme	transcription	294:306	arg1	factors					308:314	transcription factors	294:314	transcription factors	294:314	However, the role of transcription factors in regulating the starch metabolism pathway remains unclear.					
31128711	0	63	theme	ZmbZIP22	18:25	arg1	gene					27:30	ZmbZIP22 gene	18:30	ZmbZIP22 gene	18:30	Overexpression of ZmbZIP22 gene alters endosperm starch content and composition in maize and rice.					
31128711	7	64	theme	genes	840:844	arg1	transcription					805:817	the transcription	801:817	the transcription of starch metabolism genes	801:844	ZmbZIP22 promotes the transcription of starch metabolism genes by binding to their promoters.					
30625558	2	0	theme	high	540:543	arg1	density					545:551	a high density	538:551	a high density of 6.17 μmol m-2 for metal affinity groups	538:594	The successful modifications of the graphene surface were demonstrated by various characterizations, and a high density of 6.17 μmol m-2 for metal affinity groups was obtained.					
30625558	6	1	theme	Hb	991:992	arg1	capacity					979:986	The adsorption capacity	964:986	The adsorption capacity of Hb	964:992	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	2	2	theme	successful	437:446	arg1	modifications					448:460	The successful modifications	433:460	The successful modifications of the graphene surface	433:484	The successful modifications of the graphene surface were demonstrated by various characterizations, and a high density of 6.17 μmol m-2 for metal affinity groups was obtained.					
30625558	6	3	dep	affinity	1077:1084	arg1	MGI-Cu					1124:1129	MGI-Cu	1124:1129	MGI-Cu	1124:1129	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	6	3	dep	affinity	1077:1084	arg1	composite					1113:1121	modified magnetic graphene composite	1086:1121	metal affinity modified magnetic graphene composite (MGI-Cu)	1071:1130	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	7	4	theme	carbonate	1206:1214	arg1	buffer					1216:1221	a carbonate buffer	1204:1221	a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole	1204:1274	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	7	4	theme	carbonate	1206:1214	arg1	pH					1236:1237	0.2 mol L-1 pH 10	1224:1240	0.2 mol L-1 pH 10	1224:1240	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	9	5	from	lysate	1627:1632	arg1	blood					1544:1548	human whole blood	1532:1548	human whole blood	1532:1548	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	9	5	from	lysate	1627:1632	arg1	protein					1587:1593	polyhistidine-tagged recombinant protein	1554:1593	polyhistidine-tagged recombinant protein	1554:1593	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	1	6	theme	magnetic	317:324	arg1	nanoparticles					326:338	magnetic nanoparticles	317:338	magnetic nanoparticles	317:338	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	6	theme	magnetic	317:324	arg1	cellulose					355:363	carboxymethyl cellulose	341:363	carboxymethyl cellulose (CMC)	341:369	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	6	theme	magnetic	317:324	arg1	acid					386:389	iminodiacetic acid	372:389	iminodiacetic acid (IDA)	372:395	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	0	7	theme	proteins	131:138	arg1	isolation					103:111	highly selective isolation	86:111	highly selective isolation of histidine-rich proteins	86:138	Metal affinity-carboxymethyl cellulose functionalized magnetic graphene composite for highly selective isolation of histidine-rich proteins.					
30625558	3	8	theme	adsorption	639:648	arg1	selectivity					650:660	high adsorption selectivity	634:660	high adsorption selectivity toward histidine-rich proteins	634:691	The composite exhibited high adsorption selectivity toward histidine-rich proteins.					
30625558	4	9	theme	affinity	738:745	arg1	force					755:759	strong metal affinity binding force	725:759	strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite	725:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	0	10	theme	histidine-rich	116:129	arg1	proteins					131:138	histidine-rich proteins	116:138	histidine-rich proteins	116:138	Metal affinity-carboxymethyl cellulose functionalized magnetic graphene composite for highly selective isolation of histidine-rich proteins.					
30625558	6	11	theme	adsorption	968:977	arg1	capacity					979:986	The adsorption capacity	964:986	The adsorption capacity of Hb	964:992	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	7	12	theme	0.2 mol L-1	1224:1234	arg1	buffer					1216:1221	a carbonate buffer	1204:1221	a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole	1204:1274	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	7	12	theme	0.2 mol L-1	1224:1234	arg1	pH					1236:1237	0.2 mol L-1 pH 10	1224:1240	0.2 mol L-1 pH 10	1224:1240	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	4	13	theme	Cu2+	817:820	arg1	ions					822:825	immobilized Cu2+ ions	805:825	immobilized Cu2+ ions of MGCI-Cu composite	805:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	9	14	theme	polyhistidine-tagged	1554:1573	arg1	protein					1587:1593	polyhistidine-tagged recombinant protein	1554:1593	polyhistidine-tagged recombinant protein	1554:1593	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	4	15	theme	immobilized	805:815	arg1	ions					822:825	immobilized Cu2+ ions	805:825	immobilized Cu2+ ions of MGCI-Cu composite	805:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	8	16	theme	composite	1377:1385	arg1	regeneration					1357:1368	regeneration	1357:1368	regeneration of the composite by edetic acid (EDTA) and Cu2+ solution	1357:1425	MGCI-Cu composite displayed favorable reusability for at least four times after regeneration of the composite by edetic acid (EDTA) and Cu2+ solution.					
30625558	8	17	theme	MGCI-Cu	1277:1283	arg1	composite					1285:1293	MGCI-Cu composite	1277:1293	MGCI-Cu composite	1277:1293	MGCI-Cu composite displayed favorable reusability for at least four times after regeneration of the composite by edetic acid (EDTA) and Cu2+ solution.					
30625558	1	18	theme	MGCI-Cu	223:229	arg1	graphene					206:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	18	theme	MGCI-Cu	223:229	arg1	composite					231:239	MGCI-Cu composite	223:239	namely MGCI-Cu composite	216:239	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	2	19	theme	6.17 μmol m-2	556:568	arg1	density					545:551	a high density	538:551	a high density of 6.17 μmol m-2 for metal affinity groups	538:594	The successful modifications of the graphene surface were demonstrated by various characterizations, and a high density of 6.17 μmol m-2 for metal affinity groups was obtained.					
30625558	6	20	from	435 mg g-1	1057:1066	arg1	affinity					1077:1084	metal affinity modified magnetic graphene composite (MGI-Cu)	1071:1130	metal affinity modified magnetic graphene composite (MGI-Cu)	1071:1130	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	6	21	theme	graphene	1104:1111	arg1	MGI-Cu					1124:1129	MGI-Cu	1124:1129	MGI-Cu	1124:1129	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	6	21	theme	graphene	1104:1111	arg1	composite					1113:1121	modified magnetic graphene composite	1086:1121	metal affinity modified magnetic graphene composite (MGI-Cu)	1071:1130	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	4	22	theme	metal	732:736	arg1	force					755:759	strong metal affinity binding force	725:759	strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite	725:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	0	23	theme	affinity-carboxymethyl	6:27	arg1	cellulose					29:37	Metal affinity-carboxymethyl cellulose	0:37	Metal affinity-carboxymethyl cellulose	0:37	Metal affinity-carboxymethyl cellulose functionalized magnetic graphene composite for highly selective isolation of histidine-rich proteins.					
30625558	1	24	theme	copper	420:425	arg1	ions					427:430	copper ions	420:430	copper ions	420:430	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	7	25	theme	adsorbed	1162:1169	arg1	molecules					1174:1182	The adsorbed Hb molecules	1158:1182	The adsorbed Hb molecules	1158:1182	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	9	26	theme	coli	1612:1615	arg1	lysate					1627:1632	Escherichia coli (E. coli) lysate	1600:1632	Escherichia coli (E. coli) lysate	1600:1632	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	0	27	theme	Metal	0:4	arg1	cellulose					29:37	Metal affinity-carboxymethyl cellulose	0:37	Metal affinity-carboxymethyl cellulose	0:37	Metal affinity-carboxymethyl cellulose functionalized magnetic graphene composite for highly selective isolation of histidine-rich proteins.					
30625558	1	28	theme	carboxymethyl	341:353	arg1	CMC					366:368	CMC	366:368	CMC	366:368	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	28	theme	carboxymethyl	341:353	arg1	nanoparticles					326:338	magnetic nanoparticles	317:338	magnetic nanoparticles	317:338	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	28	theme	carboxymethyl	341:353	arg1	cellulose					355:363	carboxymethyl cellulose	341:363	carboxymethyl cellulose (CMC)	341:369	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	7	29	theme	Hb	1171:1172	arg1	molecules					1174:1182	The adsorbed Hb molecules	1158:1182	The adsorbed Hb molecules	1158:1182	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	5	30	theme	phosphate	938:946	arg1	buffer					948:953	0.2 mol L-1 phosphate buffer	926:953	0.2 mol L-1 phosphate buffer at pH 8	926:961	In particular, highly selective isolation of hemoglobin (Hb) was achieved in 0.2 mol L-1 phosphate buffer at pH 8.					
30625558	3	31	theme	high	634:637	arg1	selectivity					650:660	high adsorption selectivity	634:660	high adsorption selectivity toward histidine-rich proteins	634:691	The composite exhibited high adsorption selectivity toward histidine-rich proteins.					
30625558	4	32	theme	binding	747:753	arg1	force					755:759	strong metal affinity binding force	725:759	strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite	725:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	33	theme	ions	822:825	arg1	residues					780:787	hisitidine residues	769:787	hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite	769:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	33	theme	ions	822:825	arg1	ions					822:825	immobilized Cu2+ ions	805:825	immobilized Cu2+ ions of MGCI-Cu composite	805:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	33	theme	ions	822:825	arg1	proteins					792:799	proteins	792:799	proteins	792:799	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	8	34	theme	edetic	1390:1395	arg1	EDTA					1403:1406	EDTA	1403:1406	EDTA	1403:1406	MGCI-Cu composite displayed favorable reusability for at least four times after regeneration of the composite by edetic acid (EDTA) and Cu2+ solution.					
30625558	8	34	theme	edetic	1390:1395	arg1	acid					1397:1400	edetic acid	1390:1400	edetic acid (EDTA)	1390:1407	MGCI-Cu composite displayed favorable reusability for at least four times after regeneration of the composite by edetic acid (EDTA) and Cu2+ solution.					
30625558	7	35	contain	containing	1243:1252	arg2	imidazole					1266:1274	0.5 mol L-1 imidazole	1254:1274	0.5 mol L-1 imidazole	1254:1274	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	7	35	contain	containing	1243:1252	arg1	buffer					1216:1221	a carbonate buffer	1204:1221	a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole	1204:1274	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	7	35	contain	containing	1243:1252	arg1	pH					1236:1237	0.2 mol L-1 pH 10	1224:1240	0.2 mol L-1 pH 10	1224:1240	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	4	36	theme	composite	838:846	arg1	ions					822:825	immobilized Cu2+ ions	805:825	immobilized Cu2+ ions of MGCI-Cu composite	805:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	36	theme	composite	838:846	arg1	proteins					792:799	proteins	792:799	proteins	792:799	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	2	37	theme	graphene	469:476	arg1	surface					478:484	the graphene surface	465:484	the graphene surface	465:484	The successful modifications of the graphene surface were demonstrated by various characterizations, and a high density of 6.17 μmol m-2 for metal affinity groups was obtained.					
30625558	2	38	theme	surface	478:484	arg1	modifications					448:460	The successful modifications	433:460	The successful modifications of the graphene surface	433:484	The successful modifications of the graphene surface were demonstrated by various characterizations, and a high density of 6.17 μmol m-2 for metal affinity groups was obtained.					
30625558	6	39	theme	magnetic	1095:1102	arg1	MGI-Cu					1124:1129	MGI-Cu	1124:1129	MGI-Cu	1124:1129	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	6	39	theme	magnetic	1095:1102	arg1	composite					1113:1121	modified magnetic graphene composite	1086:1121	metal affinity modified magnetic graphene composite (MGI-Cu)	1071:1130	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	4	40	theme	MGCI-Cu	830:836	arg1	composite					838:846	MGCI-Cu composite	830:846	MGCI-Cu composite	830:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	41	theme	hisitidine	769:778	arg1	residues					780:787	hisitidine residues	769:787	hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite	769:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	41	theme	hisitidine	769:778	arg1	ions					822:825	immobilized Cu2+ ions	805:825	immobilized Cu2+ ions of MGCI-Cu composite	805:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	41	theme	hisitidine	769:778	arg1	proteins					792:799	proteins	792:799	proteins	792:799	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	0	42	theme	graphene	63:70	arg1	composite					72:80	magnetic graphene composite	54:80	magnetic graphene composite	54:80	Metal affinity-carboxymethyl cellulose functionalized magnetic graphene composite for highly selective isolation of histidine-rich proteins.					
30625558	4	43	theme	proteins	792:799	arg1	residues					780:787	hisitidine residues	769:787	hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite	769:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	43	theme	proteins	792:799	arg1	ions					822:825	immobilized Cu2+ ions	805:825	immobilized Cu2+ ions of MGCI-Cu composite	805:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	43	theme	proteins	792:799	arg1	proteins					792:799	proteins	792:799	proteins	792:799	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	4	44	theme	strong	725:730	arg1	force					755:759	strong metal affinity binding force	725:759	strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite	725:846	The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu2+ ions of MGCI-Cu composite.					
30625558	6	45	theme	CMC	1140:1142	arg1	modification					1144:1155	CMC modification	1140:1155	CMC modification	1140:1155	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	1	46	theme	successive	258:267	arg1	modifications					269:281	successive modifications	258:281	successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA)	258:395	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	0	47	theme	magnetic	54:61	arg1	composite					72:80	magnetic graphene composite	54:80	magnetic graphene composite	54:80	Metal affinity-carboxymethyl cellulose functionalized magnetic graphene composite for highly selective isolation of histidine-rich proteins.					
30625558	9	48	theme	practical	1432:1440	arg1	applications					1442:1453	The practical applications	1428:1453	The practical applications	1428:1453	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	5	49	theme	selective	871:879	arg1	isolation					881:889	highly selective isolation	864:889	highly selective isolation of hemoglobin (Hb)	864:908	In particular, highly selective isolation of hemoglobin (Hb) was achieved in 0.2 mol L-1 phosphate buffer at pH 8.					
30625558	6	50	theme	metal	1071:1075	arg1	affinity					1077:1084	metal affinity modified magnetic graphene composite (MGI-Cu)	1071:1130	metal affinity modified magnetic graphene composite (MGI-Cu)	1071:1130	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	3	51	theme	histidine-rich	669:682	arg1	proteins					684:691	histidine-rich proteins	669:691	histidine-rich proteins	669:691	The composite exhibited high adsorption selectivity toward histidine-rich proteins.					
30625558	5	52	theme	0.2 mol L-1	926:936	arg1	buffer					948:953	0.2 mol L-1 phosphate buffer	926:953	0.2 mol L-1 phosphate buffer at pH 8	926:961	In particular, highly selective isolation of hemoglobin (Hb) was achieved in 0.2 mol L-1 phosphate buffer at pH 8.					
30625558	2	53	theme	affinity	580:587	arg1	groups					589:594	metal affinity groups	574:594	metal affinity groups	574:594	The successful modifications of the graphene surface were demonstrated by various characterizations, and a high density of 6.17 μmol m-2 for metal affinity groups was obtained.					
30625558	8	54	theme	favorable	1305:1313	arg1	reusability					1315:1325	favorable reusability	1305:1325	favorable reusability for at least four times after regeneration of the composite by edetic acid (EDTA) and Cu2+ solution	1305:1425	MGCI-Cu composite displayed favorable reusability for at least four times after regeneration of the composite by edetic acid (EDTA) and Cu2+ solution.					
30625558	5	55	from	pH	958:959	arg1	buffer					948:953	0.2 mol L-1 phosphate buffer	926:953	0.2 mol L-1 phosphate buffer at pH 8	926:961	In particular, highly selective isolation of hemoglobin (Hb) was achieved in 0.2 mol L-1 phosphate buffer at pH 8.					
30625558	2	56	theme	metal	574:578	arg1	groups					589:594	metal affinity groups	574:594	metal affinity groups	574:594	The successful modifications of the graphene surface were demonstrated by various characterizations, and a high density of 6.17 μmol m-2 for metal affinity groups was obtained.					
30625558	9	57	theme	human	1532:1536	arg1	blood					1544:1548	human whole blood	1532:1548	human whole blood	1532:1548	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	1	58	theme	metal	143:147	arg1	graphene					206:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	58	theme	metal	143:147	arg1	composite					231:239	MGCI-Cu composite	223:239	namely MGCI-Cu composite	216:239	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	2	59	theme	various	507:513	arg1	characterizations					515:531	various characterizations	507:531	various characterizations	507:531	The successful modifications of the graphene surface were demonstrated by various characterizations, and a high density of 6.17 μmol m-2 for metal affinity groups was obtained.					
30625558	1	60	theme	iminodiacetic	372:384	arg1	nanoparticles					326:338	magnetic nanoparticles	317:338	magnetic nanoparticles	317:338	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	60	theme	iminodiacetic	372:384	arg1	IDA					392:394	IDA	392:394	IDA	392:394	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	60	theme	iminodiacetic	372:384	arg1	acid					386:389	iminodiacetic acid	372:389	iminodiacetic acid (IDA)	372:395	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	9	61	theme	recombinant	1575:1585	arg1	protein					1587:1593	polyhistidine-tagged recombinant protein	1554:1593	polyhistidine-tagged recombinant protein	1554:1593	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	1	62	theme	affinity-carboxymethyl	149:170	arg1	graphene					206:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	62	theme	affinity-carboxymethyl	149:170	arg1	composite					231:239	MGCI-Cu composite	223:239	namely MGCI-Cu composite	216:239	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	8	63	dep	acid	1397:1400	arg1	solution					1418:1425	solution	1418:1425	solution	1418:1425	MGCI-Cu composite displayed favorable reusability for at least four times after regeneration of the composite by edetic acid (EDTA) and Cu2+ solution.					
30625558	1	64	theme	cellulose	172:180	arg1	graphene					206:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	64	theme	cellulose	172:180	arg1	composite					231:239	MGCI-Cu composite	223:239	namely MGCI-Cu composite	216:239	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	9	65	theme	MGCI-Cu	1473:1479	arg1	composite					1481:1489	MGCI-Cu composite	1473:1489	MGCI-Cu composite	1473:1489	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	9	66	dep	coli	1612:1615	arg1	coli					1621:1624	E. coli	1618:1624	E. coli	1618:1624	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	1	67	theme	functionalized	182:195	arg1	graphene					206:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	67	theme	functionalized	182:195	arg1	composite					231:239	MGCI-Cu composite	223:239	namely MGCI-Cu composite	216:239	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	68	theme	graphene	286:293	arg1	nanosheets					301:310	graphene oxide nanosheets	286:310	graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA)	286:395	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	0	69	theme	selective	93:101	arg1	isolation					103:111	highly selective isolation	86:111	highly selective isolation of histidine-rich proteins	86:138	Metal affinity-carboxymethyl cellulose functionalized magnetic graphene composite for highly selective isolation of histidine-rich proteins.					
30625558	1	70	with	nanosheets	301:310	arg1	nanoparticles					326:338	magnetic nanoparticles	317:338	magnetic nanoparticles	317:338	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	70	with	nanosheets	301:310	arg1	cellulose					355:363	carboxymethyl cellulose	341:363	carboxymethyl cellulose (CMC)	341:369	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	70	with	nanosheets	301:310	arg1	acid					386:389	iminodiacetic acid	372:389	iminodiacetic acid (IDA)	372:395	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	71	theme	magnetic	197:204	arg1	graphene					206:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene	141:213	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	1	71	theme	magnetic	197:204	arg1	composite					231:239	MGCI-Cu composite	223:239	namely MGCI-Cu composite	216:239	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	9	72	theme	whole	1538:1542	arg1	blood					1544:1548	human whole blood	1532:1548	human whole blood	1532:1548	The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli (E. coli) lysate.					
30625558	1	73	theme	oxide	295:299	arg1	nanosheets					301:310	graphene oxide nanosheets	286:310	graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA)	286:395	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
30625558	6	74	theme	modified	1086:1093	arg1	MGI-Cu					1124:1129	MGI-Cu	1124:1129	MGI-Cu	1124:1129	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	6	74	theme	modified	1086:1093	arg1	composite					1113:1121	modified magnetic graphene composite	1086:1121	metal affinity modified magnetic graphene composite (MGI-Cu)	1071:1130	The adsorption capacity of Hb significantly increased to 769 mg g-1 in comparison to that of 435 mg g-1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification.					
30625558	5	75	theme	hemoglobin	894:903	arg1	isolation					881:889	highly selective isolation	864:889	highly selective isolation of hemoglobin (Hb)	864:908	In particular, highly selective isolation of hemoglobin (Hb) was achieved in 0.2 mol L-1 phosphate buffer at pH 8.					
30625558	7	76	theme	0.5 mol L-1	1254:1264	arg1	imidazole					1266:1274	0.5 mol L-1 imidazole	1254:1274	0.5 mol L-1 imidazole	1254:1274	The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L-1 pH 10) containing 0.5 mol L-1 imidazole.					
30625558	1	77	theme	nanosheets	301:310	arg1	modifications					269:281	successive modifications	258:281	successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA)	258:395	A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles, carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions.					
31158799	6	0	dep	decreased	894:902	arg1	meanwhile					997:1005	meanwhile	997:1005	meanwhile	997:1005	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	6	1	theme	cumulative	1096:1105	arg1	charge					1107:1112	the cumulative charge	1092:1112	the cumulative charge of the composite bioanode	1092:1138	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	6	1	theme	cumulative	1096:1105	arg1	18,865.8 C·m-2					1144:1157	18,865.8 C·m-2	1144:1157	18,865.8 C·m-2	1144:1157	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	8	2	from	material	1467:1474	arg1	MFC					1479:1481	MFC	1479:1481	MFC	1479:1481	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	6	3	dep	meanwhile	997:1005	arg1	charge					1107:1112	the cumulative charge	1092:1112	the cumulative charge of the composite bioanode	1092:1138	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	6	3	dep	meanwhile	997:1005	arg1	18,865.8 C·m-2					1144:1157	18,865.8 C·m-2	1144:1157	18,865.8 C·m-2	1144:1157	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	7	4	theme	sequencing	1243:1252	arg1	technology					1254:1263	High-throughput sequencing technology	1227:1263	High-throughput sequencing technology	1227:1263	High-throughput sequencing technology revealed that the ternary composite bioanode had good biocompatibility and high diversity.					
31158799	1	5	theme	electrical	246:255	arg1	energy					257:262	electrical energy	246:262	electrical energy	246:262	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	0	6	theme	microbial	90:98	arg1	cell					105:108	high-performance microbial fuel cell	73:108	high-performance microbial fuel cell	73:108	A 3D porous NCNT sponge anode modified with chitosan and Polyaniline for high-performance microbial fuel cell.					
31158799	8	7	theme	capacitive	1432:1441	arg1	material					1467:1474	a capacitive and biocompatible anode material	1430:1474	a capacitive and biocompatible anode material in MFC	1430:1481	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	6	8	theme	NCNT/sponge	807:817	arg1	electrode					819:827	the NCNT/sponge electrode	803:827	the NCNT/sponge electrode	803:827	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	0	9	theme	high-performance	73:88	arg1	cell					105:108	high-performance microbial fuel cell	73:108	high-performance microbial fuel cell	73:108	A 3D porous NCNT sponge anode modified with chitosan and Polyaniline for high-performance microbial fuel cell.					
31158799	2	10	theme	power	273:277	arg1	output					279:284	The low power output	265:284	The low power output of MFCs	265:292	The low power output of MFCs remain the bottleneck for their practical applications.					
31158799	6	11	dep	2.25 Ω	920:925	arg1	to					917:918	to	917:918	to	917:918	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	7	12	theme	ternary	1283:1289	arg1	bioanode					1301:1308	the ternary composite bioanode	1279:1308	the ternary composite bioanode	1279:1308	High-throughput sequencing technology revealed that the ternary composite bioanode had good biocompatibility and high diversity.					
31158799	2	13	theme	low	269:271	arg1	output					279:284	The low power output	265:284	The low power output of MFCs	265:292	The low power output of MFCs remain the bottleneck for their practical applications.					
31158799	3	14	theme	composite	412:420	arg1	CS-NCNT-PANI					476:487	CS-NCNT-PANI	476:487	CS-NCNT-PANI	476:487	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	3	14	theme	composite	412:420	arg1	nanotubes-polyaniline					453:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline	365:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI)	365:488	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	3	15	theme	doped	440:444	arg1	CS-NCNT-PANI					476:487	CS-NCNT-PANI	476:487	CS-NCNT-PANI	476:487	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	3	15	theme	doped	440:444	arg1	nanotubes-polyaniline					453:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline	365:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI)	365:488	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	0	16	theme	fuel	100:103	arg1	cell					105:108	high-performance microbial fuel cell	73:108	high-performance microbial fuel cell	73:108	A 3D porous NCNT sponge anode modified with chitosan and Polyaniline for high-performance microbial fuel cell.					
31158799	6	17	theme	bioanode	885:892	arg1	impedance					850:858	the charge transfer impedance	830:858	the charge transfer impedance of the ternary composite bioanode	830:892	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	7	18	contain	had	1310:1312	arg1	bioanode					1301:1308	the ternary composite bioanode	1279:1308	the ternary composite bioanode	1279:1308	High-throughput sequencing technology revealed that the ternary composite bioanode had good biocompatibility and high diversity.					
31158799	7	18	contain	had	1310:1312	arg2	diversity					1345:1353	high diversity	1340:1353	high diversity	1340:1353	High-throughput sequencing technology revealed that the ternary composite bioanode had good biocompatibility and high diversity.					
31158799	7	18	contain	had	1310:1312	arg2	biocompatibility					1319:1334	good biocompatibility	1314:1334	good biocompatibility	1314:1334	High-throughput sequencing technology revealed that the ternary composite bioanode had good biocompatibility and high diversity.					
31158799	3	19	theme	carbon	446:451	arg1	CS-NCNT-PANI					476:487	CS-NCNT-PANI	476:487	CS-NCNT-PANI	476:487	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	3	19	theme	carbon	446:451	arg1	nanotubes-polyaniline					453:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline	365:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI)	365:488	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	0	20	theme	3D	2:3	arg1	anode					24:28	A 3D porous NCNT sponge anode	0:28	A 3D porous NCNT sponge anode	0:28	A 3D porous NCNT sponge anode modified with chitosan and Polyaniline for high-performance microbial fuel cell.					
31158799	6	21	theme	composite	875:883	arg1	bioanode					885:892	the ternary composite bioanode	863:892	the ternary composite bioanode	863:892	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	1	22	theme	potential	144:152	arg1	cell					128:131	A microbial fuel cell	111:131	A microbial fuel cell (MFC)	111:137	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	1	22	theme	potential	144:152	arg1	technology					174:183	a potential bio-electrochemical technology	142:183	a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy	142:262	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	3	23	theme	novel	367:371	arg1	CS-NCNT-PANI					476:487	CS-NCNT-PANI	476:487	CS-NCNT-PANI	476:487	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	3	23	theme	novel	367:371	arg1	nanotubes-polyaniline					453:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline	365:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI)	365:488	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	8	24	theme	ternary	1384:1390	arg1	composite					1392:1400	this synthesized ternary composite	1367:1400	this synthesized ternary composite	1367:1400	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	8	24	theme	ternary	1384:1390	arg1	candidate					1417:1425	a promising candidate	1405:1425	a promising candidate as a capacitive and biocompatible anode material in MFC	1405:1481	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	8	25	theme	synthesized	1372:1382	arg1	composite					1392:1400	this synthesized ternary composite	1367:1400	this synthesized ternary composite	1367:1400	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	8	25	theme	synthesized	1372:1382	arg1	candidate					1417:1425	a promising candidate	1405:1425	a promising candidate as a capacitive and biocompatible anode material in MFC	1405:1481	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	1	26	theme	bio-electrochemical	154:172	arg1	cell					128:131	A microbial fuel cell	111:131	A microbial fuel cell (MFC)	111:137	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	1	26	theme	bio-electrochemical	154:172	arg1	technology					174:183	a potential bio-electrochemical technology	142:183	a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy	142:262	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	0	27	theme	NCNT	12:15	arg1	anode					24:28	A 3D porous NCNT sponge anode	0:28	A 3D porous NCNT sponge anode	0:28	A 3D porous NCNT sponge anode modified with chitosan and Polyaniline for high-performance microbial fuel cell.					
31158799	5	28	theme	active	688:693	arg1	area					703:706	the active surface area	684:706	the active surface area	684:706	This bioanode not only increased the active surface area and capacity but also facilitated bacterial adhesion and enrichment of microbes.					
31158799	2	29	theme	MFCs	289:292	arg1	output					279:284	The low power output	265:284	The low power output of MFCs	265:292	The low power output of MFCs remain the bottleneck for their practical applications.					
31158799	6	30	theme	charge-discharge	1056:1071	arg1	time					1073:1076	a charge-discharge time	1054:1076	a charge-discharge time of 60-60 min	1054:1089	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	0	31	theme	porous	5:10	arg1	anode					24:28	A 3D porous NCNT sponge anode	0:28	A 3D porous NCNT sponge anode	0:28	A 3D porous NCNT sponge anode modified with chitosan and Polyaniline for high-performance microbial fuel cell.					
31158799	5	32	theme	surface	695:701	arg1	area					703:706	the active surface area	684:706	the active surface area	684:706	This bioanode not only increased the active surface area and capacity but also facilitated bacterial adhesion and enrichment of microbes.					
31158799	6	33	theme	ternary	867:873	arg1	bioanode					885:892	the ternary composite bioanode	863:892	the ternary composite bioanode	863:892	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	3	34	theme	MFC	558:560	arg1	anode					562:566	an MFC anode	555:566	an MFC anode	555:566	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	7	35	theme	composite	1291:1299	arg1	bioanode					1301:1308	the ternary composite bioanode	1279:1308	the ternary composite bioanode	1279:1308	High-throughput sequencing technology revealed that the ternary composite bioanode had good biocompatibility and high diversity.					
31158799	3	36	theme	chitosan-nitrogen	422:438	arg1	CS-NCNT-PANI					476:487	CS-NCNT-PANI	476:487	CS-NCNT-PANI	476:487	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	3	36	theme	chitosan-nitrogen	422:438	arg1	nanotubes-polyaniline					453:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline	365:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI)	365:488	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	4	37	theme	ternary	632:638	arg1	composite					640:648	the ternary composite	628:648	the ternary composite	628:648	The PANI was grafted on the CS-NCNT backbone to synthesize the ternary composite.					
31158799	0	38	theme	sponge	17:22	arg1	anode					24:28	A 3D porous NCNT sponge anode	0:28	A 3D porous NCNT sponge anode	0:28	A 3D porous NCNT sponge anode modified with chitosan and Polyaniline for high-performance microbial fuel cell.					
31158799	6	39	theme	power	944:948	arg1	density					950:956	the maximum power density	932:956	the maximum power density	932:956	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	3	40	theme	bioelectrocatalytic	392:410	arg1	CS-NCNT-PANI					476:487	CS-NCNT-PANI	476:487	CS-NCNT-PANI	476:487	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	3	40	theme	bioelectrocatalytic	392:410	arg1	nanotubes-polyaniline					453:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline	365:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI)	365:488	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	8	41	theme	promising	1407:1415	arg1	composite					1392:1400	this synthesized ternary composite	1367:1400	this synthesized ternary composite	1367:1400	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	8	41	theme	promising	1407:1415	arg1	candidate					1417:1425	a promising candidate	1405:1425	a promising candidate as a capacitive and biocompatible anode material in MFC	1405:1481	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	6	42	theme	maximum	936:942	arg1	density					950:956	the maximum power density	932:956	the maximum power density	932:956	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	2	43	theme	practical	326:334	arg1	applications					336:347	their practical applications	320:347	their practical applications	320:347	The low power output of MFCs remain the bottleneck for their practical applications.					
31158799	3	44	theme	biocompatible	374:386	arg1	CS-NCNT-PANI					476:487	CS-NCNT-PANI	476:487	CS-NCNT-PANI	476:487	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	3	44	theme	biocompatible	374:386	arg1	nanotubes-polyaniline					453:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline	365:473	a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI)	365:488	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	7	45	theme	high	1340:1343	arg1	diversity					1345:1353	high diversity	1340:1353	high diversity	1340:1353	High-throughput sequencing technology revealed that the ternary composite bioanode had good biocompatibility and high diversity.					
31158799	6	46	theme	transfer	841:848	arg1	impedance					850:858	the charge transfer impedance	830:858	the charge transfer impedance of the ternary composite bioanode	830:892	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	6	47	with	experiment	1038:1047	arg1	time					1073:1076	a charge-discharge time	1054:1076	a charge-discharge time of 60-60 min	1054:1089	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	6	48	theme	bioanode	1131:1138	arg1	charge					1107:1112	the cumulative charge	1092:1112	the cumulative charge of the composite bioanode	1092:1138	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	6	48	theme	bioanode	1131:1138	arg1	18,865.8 C·m-2					1144:1157	18,865.8 C·m-2	1144:1157	18,865.8 C·m-2	1144:1157	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	8	49	theme	biocompatible	1447:1459	arg1	material					1467:1474	a capacitive and biocompatible anode material	1430:1474	a capacitive and biocompatible anode material in MFC	1430:1481	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	6	50	theme	charge	834:839	arg1	impedance					850:858	the charge transfer impedance	830:858	the charge transfer impedance of the ternary composite bioanode	830:892	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	1	51	theme	microbial	113:121	arg1	MFC					134:136	MFC	134:136	MFC	134:136	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	1	51	theme	microbial	113:121	arg1	technology					174:183	a potential bio-electrochemical technology	142:183	a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy	142:262	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	1	51	theme	microbial	113:121	arg1	cell					128:131	A microbial fuel cell	111:131	A microbial fuel cell (MFC)	111:137	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	6	52	theme	chronoamperometric	1019:1036	arg1	experiment					1038:1047	the chronoamperometric experiment	1015:1047	the chronoamperometric experiment with a charge-discharge time of 60-60 min	1015:1089	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	6	53	theme	NCNT/S	1198:1203	arg1	3625.3 C·m-2					1212:1223	3625.3 C·m-2	1212:1223	3625.3 C·m-2	1212:1223	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	6	53	theme	NCNT/S	1198:1203	arg1	anode					1205:1209	the NCNT/S anode	1194:1209	the NCNT/S anode (3625.3 C·m-2)	1194:1224	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	1	54	theme	fuel	123:126	arg1	MFC					134:136	MFC	134:136	MFC	134:136	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	1	54	theme	fuel	123:126	arg1	technology					174:183	a potential bio-electrochemical technology	142:183	a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy	142:262	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	1	54	theme	fuel	123:126	arg1	cell					128:131	A microbial fuel cell	111:131	A microbial fuel cell (MFC)	111:137	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	6	55	theme	composite	1121:1129	arg1	bioanode					1131:1138	the composite bioanode	1117:1138	the composite bioanode	1117:1138	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	5	56	theme	bacterial	742:750	arg1	adhesion					752:759	bacterial adhesion	742:759	bacterial adhesion	742:759	This bioanode not only increased the active surface area and capacity but also facilitated bacterial adhesion and enrichment of microbes.					
31158799	8	57	theme	anode	1461:1465	arg1	material					1467:1474	a capacitive and biocompatible anode material	1430:1474	a capacitive and biocompatible anode material in MFC	1430:1481	Therefore, this synthesized ternary composite is a promising candidate as a capacitive and biocompatible anode material in MFC.					
31158799	7	58	theme	good	1314:1317	arg1	biocompatibility					1319:1334	good biocompatibility	1314:1334	good biocompatibility	1314:1334	High-throughput sequencing technology revealed that the ternary composite bioanode had good biocompatibility and high diversity.					
31158799	3	59	theme	3D	518:519	arg1	NCNT/sponge					528:538	the 3D porous NCNT/sponge	514:538	the 3D porous NCNT/sponge	514:538	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	6	60	theme	60-60 min	1081:1089	arg1	time					1073:1076	a charge-discharge time	1054:1076	a charge-discharge time of 60-60 min	1054:1089	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	1	61	theme	chemical	225:232	arg1	energy					234:239	chemical energy	225:239	chemical energy	225:239	A microbial fuel cell (MFC) is a potential bio-electrochemical technology that utilizes microorganisms to convert chemical energy into electrical energy.					
31158799	6	62	dep	4.2 W·m-3	986:994	arg1	to					983:984	to	983:984	to	983:984	Compared with the NCNT/sponge electrode, the charge transfer impedance of the ternary composite bioanode decreased from 14.07 Ω to 2.25 Ω, and the maximum power density increased from 1.4 W·m-3 to 4.2 W·m-3; meanwhile, during the chronoamperometric experiment with a charge-discharge time of 60-60 min, the cumulative charge of the composite bioanode was 18,865.8 C·m-2, which is much higher than that of the NCNT/S anode (3625.3 C·m-2).					
31158799	5	63	theme	microbes	779:786	arg1	adhesion					752:759	bacterial adhesion	742:759	bacterial adhesion	742:759	This bioanode not only increased the active surface area and capacity but also facilitated bacterial adhesion and enrichment of microbes.					
31158799	5	63	theme	microbes	779:786	arg1	enrichment					765:774	enrichment	765:774	enrichment of microbes	765:786	This bioanode not only increased the active surface area and capacity but also facilitated bacterial adhesion and enrichment of microbes.					
31158799	3	64	theme	porous	521:526	arg1	NCNT/sponge					528:538	the 3D porous NCNT/sponge	514:538	the 3D porous NCNT/sponge	514:538	In this paper, a novel, biocompatible and bioelectrocatalytic composite chitosan-nitrogen doped carbon nanotubes-polyaniline (CS-NCNT-PANI) was prepared in situ on the 3D porous NCNT/sponge and applied to an MFC anode.					
31158799	7	65	theme	High-throughput	1227:1241	arg1	technology					1254:1263	High-throughput sequencing technology	1227:1263	High-throughput sequencing technology	1227:1263	High-throughput sequencing technology revealed that the ternary composite bioanode had good biocompatibility and high diversity.					
31158799	4	66	theme	CS-NCNT	597:603	arg1	backbone					605:612	the CS-NCNT backbone	593:612	the CS-NCNT backbone to synthesize the ternary composite	593:648	The PANI was grafted on the CS-NCNT backbone to synthesize the ternary composite.					
29973701	7	0	theme	chow	1259:1262	arg1	mice					1264:1267	the normal chow mice	1248:1267	the normal chow mice	1248:1267	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	4	1	theme	isoquercetin + 5	866:881	arg1	inulin					884:889	(v) HF diet + 0.05% isoquercetin + 5% inulin	846:889	(v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10)	846:898	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	1	theme	isoquercetin + 5	866:881	arg1	n = 10					892:897	n = 10	892:897	n = 10	892:897	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	8	2	theme	lipid	1471:1475	arg1	accumulation					1477:1488	reduced hepatic lipid accumulation	1455:1488	reduced hepatic lipid accumulation	1455:1488	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	2	3	from	effects	346:352	arg1	parameters					402:411	cardiometabolic health parameters	379:411	cardiometabolic health parameters	379:411	These synergistic activities facilitate the beneficial effects of dietary flavonoids on cardiometabolic health parameters.					
29973701	7	4	theme	glucose	1194:1200	arg1	tolerance					1202:1210	impaired glucose tolerance	1185:1210	impaired glucose tolerance	1185:1210	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	10	5	theme	soluble	1859:1865	arg1	inulin					1873:1878	the soluble fibre inulin	1855:1878	the soluble fibre inulin	1855:1878	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	8	6	theme	glucose	1412:1418	arg1	tolerance					1420:1428	improved glucose tolerance	1403:1428	improved glucose tolerance	1403:1428	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	6	7	theme	skeletal	1050:1057	arg1	muscle					1059:1064	skeletal muscle	1050:1064	skeletal muscle	1050:1064	At 12 weeks, glucose and insulin tolerance tests were performed, and blood, faecal samples, liver, skeletal muscle and adipose tissue were collected.					
29973701	1	8	theme	bioactive	268:276	arg1	metabolites					278:288	bioactive metabolites	268:288	bioactive metabolites	268:288	Dietary fibre positively influences gut microbiome composition, enhancing the metabolism of dietary flavonoids to produce bioactive metabolites.					
29973701	9	9	from	improvements	1646:1657	arg1	functionality					1682:1694	functionality	1682:1694	functionality	1682:1694	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	9	9	from	improvements	1646:1657	arg1	composition					1666:1676	composition	1666:1676	composition	1666:1676	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	7	10	contain	had	1134:1136	arg2	tolerance					1202:1210	impaired glucose tolerance	1185:1210	impaired glucose tolerance	1185:1210	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	7	10	contain	had	1134:1136	arg1	mice					1114:1117	mice	1114:1117	mice on the HF diet	1114:1132	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	7	10	contain	had	1134:1136	arg2	weights					1166:1172	significantly elevated body weights	1138:1172	significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity	1138:1234	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	7	11	theme	insulin	1216:1222	arg1	sensitivity					1224:1234	insulin sensitivity	1216:1234	insulin sensitivity	1216:1234	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	8	12	theme	circulating	1514:1524	arg1	leptin					1526:1531	circulating leptin	1514:1531	circulating leptin	1514:1531	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	1	13	theme	gut	182:184	arg1	composition					197:207	gut microbiome composition	182:207	gut microbiome composition	182:207	Dietary fibre positively influences gut microbiome composition, enhancing the metabolism of dietary flavonoids to produce bioactive metabolites.					
29973701	7	14	theme	body	1161:1164	arg1	weights					1166:1172	significantly elevated body weights	1138:1172	significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity	1138:1234	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	9	15	theme	microbiome	1709:1718	arg1	functionality					1682:1694	functionality	1682:1694	functionality	1682:1694	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	9	15	theme	microbiome	1709:1718	arg1	composition					1666:1676	composition	1666:1676	composition	1666:1676	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	0	16	from	development	79:89	arg1	mice					120:123	mice	120:123	mice fed a high fat diet	120:143	Isoquercetin and inulin synergistically modulate the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat diet.					
29973701	6	17	theme	faecal	1027:1032	arg1	samples					1034:1040	faecal samples	1027:1040	faecal samples	1027:1040	At 12 weeks, glucose and insulin tolerance tests were performed, and blood, faecal samples, liver, skeletal muscle and adipose tissue were collected.					
29973701	10	18	theme	metabolic	1913:1921	arg1	syndrome					1923:1930	the metabolic syndrome	1909:1930	the metabolic syndrome	1909:1930	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	4	19	theme	male	651:654	arg1	mice					663:666	male C57BL6 mice	651:666	male C57BL6 mice (6-8 weeks)	651:678	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	19	theme	male	651:654	arg1	weeks					673:677	6-8 weeks	669:677	6-8 weeks	669:677	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	3	20	theme	syndrome	605:612	arg1	features					579:586	features	579:586	features of the metabolic syndrome	579:612	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	8	21	theme	FGF21	1545:1549	arg1	levels					1551:1556	adipose FGF21 levels	1537:1556	adipose FGF21 levels	1537:1556	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	4	22	theme	HF	778:779	arg1	n = 10					808:813	n = 10	808:813	n = 10	808:813	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	22	theme	HF	778:779	arg1	isoquercetin					794:805	(iii) HF diet + 0.05% isoquercetin	772:805	(iii) HF diet + 0.05% isoquercetin (n = 10)	772:814	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	10	23	theme	high	1946:1949	arg1	diet					1955:1958	a high fat diet	1944:1958	a high fat diet	1944:1958	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	0	24	theme	fat	136:138	arg1	diet					140:143	a high fat diet	129:143	a high fat diet	129:143	Isoquercetin and inulin synergistically modulate the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat diet.					
29973701	4	25	theme	%	792:792	arg1	n = 10					808:813	n = 10	808:813	n = 10	808:813	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	25	theme	%	792:792	arg1	isoquercetin					794:805	(iii) HF diet + 0.05% isoquercetin	772:805	(iii) HF diet + 0.05% isoquercetin (n = 10)	772:814	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	9	26	theme	short	1745:1749	arg1	acids					1763:1767	short chain fatty acids	1745:1767	short chain fatty acids	1745:1767	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	10	27	theme	long-term	1785:1793	arg1	supplementation					1795:1809	long-term supplementation	1785:1809	long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin	1785:1878	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	6	28	theme	tolerance	984:992	arg1	tests					994:998	glucose and insulin tolerance tests	964:998	glucose and insulin tolerance tests	964:998	At 12 weeks, glucose and insulin tolerance tests were performed, and blood, faecal samples, liver, skeletal muscle and adipose tissue were collected.					
29973701	4	29	theme	diet + 5	825:832	arg1	inulin					835:840	(iv) HF diet + 5% inulin	817:840	(iv) HF diet + 5% inulin	817:840	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	9	30	theme	fatty	1757:1761	arg1	acids					1763:1767	short chain fatty acids	1745:1767	short chain fatty acids	1745:1767	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	10	31	theme	dietary	1820:1826	arg1	isoquercetin					1838:1849	the dietary flavonoid isoquercetin	1816:1849	the dietary flavonoid isoquercetin	1816:1849	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	8	32	theme	HF	1590:1591	arg1	diet					1593:1596	the HF diet	1586:1596	the HF diet	1586:1596	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	4	33	dep	diet	723:726	arg1	i					708:708	i	708:708	i	708:708	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	7	34	from	mice	1114:1117	arg1	diet					1129:1132	the HF diet	1122:1132	the HF diet	1122:1132	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	0	35	theme	syndrome	108:115	arg1	development					79:89	development	79:89	development of the metabolic syndrome in mice fed a high fat diet	79:143	Isoquercetin and inulin synergistically modulate the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat diet.					
29973701	5	36	theme	food	917:920	arg1	intake					922:927	food intake	917:927	food intake	917:927	Body weight and food intake were measured weekly.					
29973701	4	37	theme	HF	850:851	arg1	inulin					884:889	(v) HF diet + 0.05% isoquercetin + 5% inulin	846:889	(v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10)	846:898	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	37	theme	HF	850:851	arg1	n = 10					892:897	n = 10	892:897	n = 10	892:897	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	38	theme	%	864:864	arg1	inulin					884:889	(v) HF diet + 0.05% isoquercetin + 5% inulin	846:889	(v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10)	846:898	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	38	theme	%	864:864	arg1	n = 10					892:897	n = 10	892:897	n = 10	892:897	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	8	39	contain	had	1375:1377	arg2	accumulation					1477:1488	reduced hepatic lipid accumulation	1455:1488	reduced hepatic lipid accumulation	1455:1488	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	8	39	contain	had	1375:1377	arg2	hypertrophy					1501:1511	adipocyte hypertrophy	1491:1511	adipocyte hypertrophy	1491:1511	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	8	39	contain	had	1375:1377	arg1	mice					1344:1347	mice	1344:1347	mice receiving the combination	1344:1373	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	8	39	contain	had	1375:1377	arg2	levels					1551:1556	adipose FGF21 levels	1537:1556	adipose FGF21 levels	1537:1556	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	8	39	contain	had	1375:1377	arg2	leptin					1526:1531	circulating leptin	1514:1531	circulating leptin	1514:1531	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	8	39	contain	had	1375:1377	arg2	tolerance					1420:1428	improved glucose tolerance	1403:1428	improved glucose tolerance	1403:1428	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	8	39	contain	had	1375:1377	arg2	gain					1397:1400	attenuated weight gain	1379:1400	attenuated weight gain	1379:1400	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	4	40	theme	chow	718:721	arg1	n = 10					729:734	n = 10	729:734	n = 10	729:734	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	40	theme	chow	718:721	arg1	diet					723:726	(i) normal chow diet	707:726	(i) normal chow diet (n = 10)	707:735	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	10	41	from	development	1894:1904	arg1	mice					1935:1938	mice	1935:1938	mice fed a high fat diet	1935:1958	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	3	42	theme	dietary	487:493	arg1	isoquercetin					465:476	isoquercetin	465:476	isoquercetin (a major dietary flavonoid)	465:504	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	3	42	theme	dietary	487:493	arg1	flavonoid					495:503	a major dietary flavonoid	479:503	a major dietary flavonoid	479:503	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	4	43	dep	assigned	694:701	arg1	n = 10					808:813	n = 10	808:813	n = 10	808:813	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	43	dep	assigned	694:701	arg1	diet					757:760	(ii) high fat (HF) diet	738:760	(ii) high fat (HF) diet (n = 10)	738:769	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	43	dep	assigned	694:701	arg1	diet					723:726	(i) normal chow diet	707:726	(i) normal chow diet (n = 10)	707:735	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	43	dep	assigned	694:701	arg1	inulin					884:889	(v) HF diet + 0.05% isoquercetin + 5% inulin	846:889	(v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10)	846:898	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	43	dep	assigned	694:701	arg1	n = 10					763:768	n = 10	763:768	n = 10	763:768	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	43	dep	assigned	694:701	arg1	n = 10					729:734	n = 10	729:734	n = 10	729:734	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	43	dep	assigned	694:701	arg1	inulin					835:840	(iv) HF diet + 5% inulin	817:840	(iv) HF diet + 5% inulin	817:840	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	43	dep	assigned	694:701	arg1	n = 10					892:897	n = 10	892:897	n = 10	892:897	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	43	dep	assigned	694:701	arg1	isoquercetin					794:805	(iii) HF diet + 0.05% isoquercetin	772:805	(iii) HF diet + 0.05% isoquercetin (n = 10)	772:814	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	44	theme	fat	748:750	arg1	n = 10					763:768	n = 10	763:768	n = 10	763:768	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	44	theme	fat	748:750	arg1	diet					757:760	(ii) high fat (HF) diet	738:760	(ii) high fat (HF) diet (n = 10)	738:769	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	9	45	from	production	1731:1740	arg1	functionality					1682:1694	functionality	1682:1694	functionality	1682:1694	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	9	45	from	production	1731:1740	arg1	composition					1666:1676	composition	1666:1676	composition	1666:1676	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	4	46	theme	HF	753:754	arg1	n = 10					763:768	n = 10	763:768	n = 10	763:768	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	46	theme	HF	753:754	arg1	diet					757:760	(ii) high fat (HF) diet	738:760	(ii) high fat (HF) diet (n = 10)	738:769	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	2	47	theme	flavonoids	365:374	arg1	effects					346:352	the beneficial effects	331:352	the beneficial effects of dietary flavonoids on cardiometabolic health parameters	331:411	These synergistic activities facilitate the beneficial effects of dietary flavonoids on cardiometabolic health parameters.					
29973701	0	48	theme	gut	53:55	arg1	microbiome					57:66	the gut microbiome	49:66	the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat diet	49:143	Isoquercetin and inulin synergistically modulate the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat diet.					
29973701	9	49	theme	acids	1763:1767	arg1	production					1731:1740	production	1731:1740	improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids	1646:1767	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	9	49	theme	acids	1763:1767	arg1	improvements					1646:1657	improvements	1646:1657	improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids	1646:1767	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	7	50	theme	impaired	1185:1192	arg1	tolerance					1202:1210	impaired glucose tolerance	1185:1210	impaired glucose tolerance	1185:1210	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	3	51	theme	major	481:485	arg1	isoquercetin					465:476	isoquercetin	465:476	isoquercetin (a major dietary flavonoid)	465:504	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	3	51	theme	major	481:485	arg1	flavonoid					495:503	a major dietary flavonoid	479:503	a major dietary flavonoid	479:503	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	4	52	theme	1	627:627	arg1	week					629:632	week	629:632	week	629:632	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	8	53	theme	insulin	1434:1440	arg1	sensitivity					1442:1452	insulin sensitivity	1434:1452	insulin sensitivity	1434:1452	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	7	54	theme	normal	1252:1257	arg1	mice					1264:1267	the normal chow mice	1248:1267	the normal chow mice	1248:1267	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	4	55	theme	%	882:882	arg1	inulin					884:889	(v) HF diet + 0.05% isoquercetin + 5% inulin	846:889	(v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10)	846:898	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	55	theme	%	882:882	arg1	n = 10					892:897	n = 10	892:897	n = 10	892:897	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	8	56	theme	attenuated	1379:1388	arg1	gain					1397:1400	attenuated weight gain	1379:1400	attenuated weight gain	1379:1400	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	6	57	theme	adipose	1070:1076	arg1	tissue					1078:1083	adipose tissue	1070:1083	adipose tissue	1070:1083	At 12 weeks, glucose and insulin tolerance tests were performed, and blood, faecal samples, liver, skeletal muscle and adipose tissue were collected.					
29973701	2	58	theme	cardiometabolic	379:393	arg1	parameters					402:411	cardiometabolic health parameters	379:411	cardiometabolic health parameters	379:411	These synergistic activities facilitate the beneficial effects of dietary flavonoids on cardiometabolic health parameters.					
29973701	1	59	theme	flavonoids	246:255	arg1	metabolism					224:233	the metabolism	220:233	the metabolism of dietary flavonoids to produce bioactive metabolites	220:288	Dietary fibre positively influences gut microbiome composition, enhancing the metabolism of dietary flavonoids to produce bioactive metabolites.					
29973701	10	60	theme	fibre	1867:1871	arg1	inulin					1873:1878	the soluble fibre inulin	1855:1878	the soluble fibre inulin	1855:1878	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	2	61	theme	synergistic	297:307	arg1	activities					309:318	These synergistic activities	291:318	These synergistic activities	291:318	These synergistic activities facilitate the beneficial effects of dietary flavonoids on cardiometabolic health parameters.					
29973701	8	62	theme	hepatic	1463:1469	arg1	accumulation					1477:1488	reduced hepatic lipid accumulation	1455:1488	reduced hepatic lipid accumulation	1455:1488	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	6	63	theme	insulin	976:982	arg1	tests					994:998	glucose and insulin tolerance tests	964:998	glucose and insulin tolerance tests	964:998	At 12 weeks, glucose and insulin tolerance tests were performed, and blood, faecal samples, liver, skeletal muscle and adipose tissue were collected.					
29973701	11	64	theme	beneficial	2025:2034	arg1	changes					2036:2042	beneficial changes	2025:2042	beneficial changes to the microbiome	2025:2060	This protective effect appears to be mediated, in part, through beneficial changes to the microbiome.					
29973701	8	65	theme	improved	1403:1410	arg1	tolerance					1420:1428	improved glucose tolerance	1403:1428	improved glucose tolerance	1403:1428	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	6	66	theme	glucose	964:970	arg1	tests					994:998	glucose and insulin tolerance tests	964:998	glucose and insulin tolerance tests	964:998	At 12 weeks, glucose and insulin tolerance tests were performed, and blood, faecal samples, liver, skeletal muscle and adipose tissue were collected.					
29973701	4	67	theme	C57BL6	656:661	arg1	mice					663:666	male C57BL6 mice	651:666	male C57BL6 mice (6-8 weeks)	651:678	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	67	theme	C57BL6	656:661	arg1	weeks					673:677	6-8 weeks	669:677	6-8 weeks	669:677	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	2	68	theme	beneficial	335:344	arg1	effects					346:352	the beneficial effects	331:352	the beneficial effects of dietary flavonoids on cardiometabolic health parameters	331:411	These synergistic activities facilitate the beneficial effects of dietary flavonoids on cardiometabolic health parameters.					
29973701	8	69	theme	adipocyte	1491:1499	arg1	hypertrophy					1501:1511	adipocyte hypertrophy	1491:1511	adipocyte hypertrophy	1491:1511	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	10	70	with	supplementation	1795:1809	arg1	inulin					1873:1878	the soluble fibre inulin	1855:1878	the soluble fibre inulin	1855:1878	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	10	70	with	supplementation	1795:1809	arg1	isoquercetin					1838:1849	the dietary flavonoid isoquercetin	1816:1849	the dietary flavonoid isoquercetin	1816:1849	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	9	71	theme	gut	1705:1707	arg1	microbiome					1709:1718	their gut microbiome	1699:1718	their gut microbiome	1699:1718	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	1	72	theme	microbiome	186:195	arg1	composition					197:207	gut microbiome composition	182:207	gut microbiome composition	182:207	Dietary fibre positively influences gut microbiome composition, enhancing the metabolism of dietary flavonoids to produce bioactive metabolites.					
29973701	10	73	theme	syndrome	1923:1930	arg1	development					1894:1904	development	1894:1904	development of the metabolic syndrome in mice fed a high fat diet	1894:1958	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	4	74	theme	week	629:632	arg1	acclimatization					634:648	a 1 week acclimatization	625:648	a 1 week acclimatization	625:648	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	0	75	theme	high	131:134	arg1	diet					140:143	a high fat diet	129:143	a high fat diet	129:143	Isoquercetin and inulin synergistically modulate the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat diet.					
29973701	3	76	theme	metabolic	595:603	arg1	syndrome					605:612	the metabolic syndrome	591:612	the metabolic syndrome	591:612	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	7	77	theme	elevated	1152:1159	arg1	weights					1166:1172	significantly elevated body weights	1138:1172	significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity	1138:1234	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	10	78	theme	fat	1951:1953	arg1	diet					1955:1958	a high fat diet	1944:1958	a high fat diet	1944:1958	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	4	79	dep	inulin	835:840	arg1	iv					818:819	iv	818:819	iv	818:819	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	5	80	theme	Body	901:904	arg1	weight					906:911	Body weight	901:911	Body weight	901:911	Body weight and food intake were measured weekly.					
29973701	8	81	theme	adipose	1537:1543	arg1	levels					1551:1556	adipose FGF21 levels	1537:1556	adipose FGF21 levels	1537:1556	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	3	82	dep	either	534:539	arg1	alone					541:545	alone	541:545	alone	541:545	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	4	83	theme	diet + 0.05	781:791	arg1	n = 10					808:813	n = 10	808:813	n = 10	808:813	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	83	theme	diet + 0.05	781:791	arg1	isoquercetin					794:805	(iii) HF diet + 0.05% isoquercetin	772:805	(iii) HF diet + 0.05% isoquercetin (n = 10)	772:814	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	7	84	theme	HF	1126:1127	arg1	diet					1129:1132	the HF diet	1122:1132	the HF diet	1122:1132	At 12 weeks, mice on the HF diet had significantly elevated body weights as well as impaired glucose tolerance and insulin sensitivity compared to the normal chow mice.					
29973701	9	85	theme	chain	1751:1755	arg1	acids					1763:1767	short chain fatty acids	1745:1767	short chain fatty acids	1745:1767	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	4	86	theme	HF	822:823	arg1	inulin					835:840	(iv) HF diet + 5% inulin	817:840	(iv) HF diet + 5% inulin	817:840	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	8	87	contain	had	1321:1323	arg1	Supplementation					1270:1284	Supplementation	1270:1284	Supplementation with either isoquercetin or inulin	1270:1319	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	8	87	contain	had	1321:1323	arg2	effect					1328:1333	no effect	1325:1333	no effect	1325:1333	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	4	88	theme	%	833:833	arg1	inulin					835:840	(iv) HF diet + 5% inulin	817:840	(iv) HF diet + 5% inulin	817:840	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	0	89	theme	metabolic	98:106	arg1	syndrome					108:115	the metabolic syndrome	94:115	the metabolic syndrome	94:115	Isoquercetin and inulin synergistically modulate the gut microbiome to prevent development of the metabolic syndrome in mice fed a high fat diet.					
29973701	4	90	dep	inulin	884:889	arg1	v					847:847	v	847:847	v	847:847	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	3	91	dep	inulin	510:515	arg1	fibre					526:530	fibre	526:530	fibre	526:530	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	8	92	with	Supplementation	1270:1284	arg1	isoquercetin					1298:1309	isoquercetin	1298:1309	isoquercetin	1298:1309	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	8	92	with	Supplementation	1270:1284	arg1	inulin					1314:1319	inulin	1314:1319	inulin	1314:1319	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	9	93	dep	composition	1666:1676	arg1	the					1662:1664	the	1662:1664	the	1662:1664	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	4	94	dep	diet	757:760	arg1	ii					739:740	ii	739:740	ii	739:740	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	9	95	contain	had	1642:1644	arg1	mice					1613:1616	mice	1613:1616	mice on the combination diet	1613:1640	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	9	95	contain	had	1642:1644	arg2	improvements					1646:1657	improvements	1646:1657	improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids	1646:1767	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	9	95	contain	had	1642:1644	arg2	production					1731:1740	production	1731:1740	improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids	1646:1767	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	4	96	theme	normal	711:716	arg1	n = 10					729:734	n = 10	729:734	n = 10	729:734	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	96	theme	normal	711:716	arg1	diet					723:726	(i) normal chow diet	707:726	(i) normal chow diet (n = 10)	707:735	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	11	97	theme	protective	1966:1975	arg1	effect					1977:1982	This protective effect	1961:1982	This protective effect	1961:1982	This protective effect appears to be mediated, in part, through beneficial changes to the microbiome.					
29973701	4	98	theme	high	743:746	arg1	n = 10					763:768	n = 10	763:768	n = 10	763:768	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	98	theme	high	743:746	arg1	diet					757:760	(ii) high fat (HF) diet	738:760	(ii) high fat (HF) diet (n = 10)	738:769	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	4	99	dep	isoquercetin	794:805	arg1	iii					773:775	iii	773:775	iii	773:775	Following a 1 week acclimatization, male C57BL6 mice (6-8 weeks) were randomly assigned to; (i) normal chow diet (n = 10), (ii) high fat (HF) diet (n = 10), (iii) HF diet + 0.05% isoquercetin (n = 10), (iv) HF diet + 5% inulin, or (v) HF diet + 0.05% isoquercetin + 5% inulin (n = 10).					
29973701	3	100	dep	isoquercetin	465:476	arg1	either					534:539	either	534:539	either	534:539	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	3	100	dep	isoquercetin	465:476	arg1	combination					553:563	combination	553:563	combination	553:563	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	3	101	theme	study	431:435	arg1	aims					418:421	The aims	414:421	The aims of this study	414:435	The aims of this study were to investigate whether isoquercetin (a major dietary flavonoid) and inulin (soluble fibre), either alone or in combination could improve features of the metabolic syndrome.					
29973701	9	102	from	mice	1613:1616	arg1	diet					1637:1640	the combination diet	1621:1640	the combination diet	1621:1640	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	2	103	theme	dietary	357:363	arg1	flavonoids					365:374	dietary flavonoids	357:374	dietary flavonoids	357:374	These synergistic activities facilitate the beneficial effects of dietary flavonoids on cardiometabolic health parameters.					
29973701	9	104	theme	combination	1625:1635	arg1	diet					1637:1640	the combination diet	1621:1640	the combination diet	1621:1640	Additionally, mice on the combination diet had improvements in the composition and functionality of their gut microbiome as well as production of short chain fatty acids.					
29973701	10	105	theme	flavonoid	1828:1836	arg1	isoquercetin					1838:1849	the dietary flavonoid isoquercetin	1816:1849	the dietary flavonoid isoquercetin	1816:1849	In conclusion, long-term supplementation with the dietary flavonoid isoquercetin and the soluble fibre inulin can attenuate development of the metabolic syndrome in mice fed a high fat diet.					
29973701	8	106	theme	weight	1390:1395	arg1	gain					1397:1400	attenuated weight gain	1379:1400	attenuated weight gain	1379:1400	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	2	107	theme	health	395:400	arg1	parameters					402:411	cardiometabolic health parameters	379:411	cardiometabolic health parameters	379:411	These synergistic activities facilitate the beneficial effects of dietary flavonoids on cardiometabolic health parameters.					
29973701	8	108	theme	reduced	1455:1461	arg1	accumulation					1477:1488	reduced hepatic lipid accumulation	1455:1488	reduced hepatic lipid accumulation	1455:1488	Supplementation with either isoquercetin or inulin had no effect, however mice receiving the combination had attenuated weight gain, improved glucose tolerance and insulin sensitivity, reduced hepatic lipid accumulation, adipocyte hypertrophy, circulating leptin and adipose FGF21 levels, compared to mice receiving the HF diet.					
29973701	1	109	theme	dietary	238:244	arg1	flavonoids					246:255	dietary flavonoids	238:255	dietary flavonoids	238:255	Dietary fibre positively influences gut microbiome composition, enhancing the metabolism of dietary flavonoids to produce bioactive metabolites.					
30971625	0	0	theme	reduction	86:94	arg1	removal					59:65	removal	59:65	removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid	59:179	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	4	1	theme	levels	934:939	arg1	increase					911:918	an additive increase	899:918	an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone	899:1070	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	2	2	from	polymerization	400:413	arg1	membrane					541:548	the plasma membrane	530:548	the plasma membrane	530:548	LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane.					
30971625	1	3	theme	lipoteichoic	353:364	arg1	acid					366:369	lipoteichoic acid	353:369	lipoteichoic acid (LTA)	353:375	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	1	3	theme	lipoteichoic	353:364	arg1	LTA					372:374	LTA	372:374	LTA	372:374	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	4	4	theme	activation	923:932	arg1	levels					934:939	activation levels	923:939	activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone	923:1070	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	1	5	theme	absence	342:348	arg1	reduction					267:275	reduction	267:275	reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA)	267:375	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	4	6	from	ugtP	827:830	arg1	cells					874:878	cells	874:878	cells lacking LTA	874:890	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	0	7	dep	Bacillus	131:138	arg1	subtilis					140:147	subtilis	140:147	subtilis	140:147	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	3	8	theme	ensuing	790:796	arg1	change					798:803	an ensuing change	787:803	an ensuing change in LTA	787:810	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	0	9	theme	phosphatidylglycerol	99:118	arg1	content					120:126	phosphatidylglycerol content	99:126	phosphatidylglycerol content	99:126	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	4	10	from	Disruption	813:822	arg1	cells					874:878	cells	874:878	cells lacking LTA	874:890	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	4	11	dep	σM	944:945	arg1	2.1-					975:978	2.1-	975:978	2.1-	975:978	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	4	11	dep	σM	944:945	arg1	2.2-					969:972	2.2-	969:972	2.2-	969:972	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	4	11	dep	σM	944:945	arg1	3.1-					963:966	3.1-	963:966	3.1-	963:966	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	6	12	theme	reduction	1311:1319	arg1	alteration					1361:1370	not the possible secondary alteration	1334:1370	not the possible secondary alteration in LTA	1334:1377	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	6	12	theme	reduction	1311:1319	arg1	absence					1285:1291	absence	1285:1291	absence of glucolipids or reduction of PG alone	1285:1331	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	6	13	from	alteration	1361:1370	arg1	LTA					1375:1377	LTA	1375:1377	LTA	1375:1377	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	6	14	theme	secondary	1351:1359	arg1	alteration					1361:1370	not the possible secondary alteration	1334:1370	not the possible secondary alteration in LTA	1334:1377	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	6	14	theme	secondary	1351:1359	arg1	absence					1285:1291	absence	1285:1291	absence of glucolipids or reduction of PG alone	1285:1331	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	5	15	theme	content	1089:1095	arg1	Reduction					1073:1081	Reduction	1073:1081	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA	1073:1147	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	0	16	from	removal	59:65	arg1	cells					149:153	Bacillus subtilis cells	131:153	Bacillus subtilis cells lacking lipoteichoic acid	131:179	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	4	17	theme	ugtP	827:830	arg1	Disruption					813:822	Disruption	813:822	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA	813:890	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	6	18	theme	factors	1450:1456	arg1	systems					1424:1430	the regulation systems	1409:1430	the regulation systems of some ECF sigma factors	1409:1456	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	1	19	theme	phosphatidylglycerol	280:299	arg1	reduction					267:275	reduction	267:275	reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA)	267:375	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	4	20	theme	additive	902:909	arg1	increase					911:918	an additive increase	899:918	an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone	899:1070	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	1	21	theme	acid	366:369	arg1	absence					342:348	absence	342:348	absence of lipoteichoic acid (LTA)	342:375	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	1	21	theme	acid	366:369	arg1	absence					315:321	absence	315:321	absence of glucolipids	315:336	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	1	21	theme	acid	366:369	arg1	content					306:312	content	306:312	content	306:312	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	1	21	theme	acid	366:369	arg1	PG					302:303	PG	302:303	PG	302:303	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	1	21	theme	acid	366:369	arg1	phosphatidylglycerol					280:299	phosphatidylglycerol	280:299	phosphatidylglycerol	280:299	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	6	22	theme	sigma	1444:1448	arg1	factors					1450:1456	some ECF sigma factors	1435:1456	some ECF sigma factors	1435:1456	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	4	23	theme	relative	1009:1016	arg1	σM					944:945	σM	944:945	σM	944:945	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	0	24	theme	content	120:126	arg1	reduction					86:94	reduction	86:94	reduction	86:94	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	0	24	theme	content	120:126	arg1	glucolipids					70:80	glucolipids	70:80	glucolipids	70:80	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	4	25	theme	σM	944:945	arg1	levels					934:939	activation levels	923:939	activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone	923:1070	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	6	26	theme	ECF	1440:1442	arg1	factors					1450:1456	some ECF sigma factors	1435:1456	some ECF sigma factors	1435:1456	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	3	27	theme	factors	760:766	arg1	activation					736:745	the activation	732:745	the activation of ECF sigma factors	732:766	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	4	28	theme	activation	1027:1036	arg1	levels					1038:1043	their activation levels	1021:1043	their activation levels in cells lacking LTA alone	1021:1070	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	4	29	theme	σV	952:953	arg1	levels					934:939	activation levels	923:939	activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone	923:1070	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	4	30	theme	glucolipid	849:858	arg1	synthesis					860:868	glucolipid synthesis	849:868	glucolipid synthesis	849:868	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	6	31	theme	glucolipids	1296:1306	arg1	alteration					1361:1370	not the possible secondary alteration	1334:1370	not the possible secondary alteration in LTA	1334:1377	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	6	31	theme	glucolipids	1296:1306	arg1	absence					1285:1291	absence	1285:1291	absence of glucolipids or reduction of PG alone	1285:1331	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	3	32	from	changes	624:630	arg1	LTA					635:637	LTA	635:637	LTA	635:637	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	3	33	from	change	798:803	arg1	LTA					808:810	LTA	808:810	LTA	808:810	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	3	34	theme	PG	687:688	arg1	content					690:696	PG content	687:696	PG content	687:696	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	2	35	theme	moiety	439:444	arg1	polymerization					400:413	polymerization	400:413	polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane	400:548	LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane.					
30971625	3	36	theme	absence	701:707	arg1	reduction					674:682	reduction	674:682	reduction of PG content or absence of glucolipids	674:722	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	6	37	theme	possible	1342:1349	arg1	alteration					1361:1370	not the possible secondary alteration	1334:1370	not the possible secondary alteration in LTA	1334:1377	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	6	37	theme	possible	1342:1349	arg1	absence					1285:1291	absence	1285:1291	absence of glucolipids or reduction of PG alone	1285:1331	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	1	38	theme	extracytoplasmic	204:219	arg1	factors					242:248	extracytoplasmic function (ECF) sigma factors	204:248	extracytoplasmic function (ECF) sigma factors	204:248	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	0	39	theme	function	31:38	arg1	factors					46:52	extracytoplasmic function sigma factors	14:52	extracytoplasmic function sigma factors	14:52	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	1	40	theme	function	221:228	arg1	factors					242:248	extracytoplasmic function (ECF) sigma factors	204:248	extracytoplasmic function (ECF) sigma factors	204:248	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	0	41	theme	extracytoplasmic	14:29	arg1	factors					46:52	extracytoplasmic function sigma factors	14:52	extracytoplasmic function sigma factors	14:52	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	3	42	theme	content	690:696	arg1	reduction					674:682	reduction	674:682	reduction of PG content or absence of glucolipids	674:722	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	0	43	theme	Bacillus	131:138	arg1	cells					149:153	Bacillus subtilis cells	131:153	Bacillus subtilis cells lacking lipoteichoic acid	131:179	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	0	44	from	reduction	86:94	arg1	cells					149:153	Bacillus subtilis cells	131:153	Bacillus subtilis cells lacking lipoteichoic acid	131:179	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	5	45	theme	levels	1191:1196	arg1	increase					1168:1175	an additive increase	1156:1175	an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively)	1156:1253	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	5	46	theme	activation	1180:1189	arg1	levels					1191:1196	activation levels	1180:1196	activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively)	1180:1253	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	0	47	theme	factors	46:52	arg1	Activation					0:9	Activation	0:9	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.	0:180	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	1	48	theme	content	306:312	arg1	reduction					267:275	reduction	267:275	reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA)	267:375	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	4	49	from	cells	874:878	arg1	Disruption					813:822	Disruption	813:822	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA	813:890	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	4	50	theme	σY	959:960	arg1	levels					934:939	activation levels	923:939	activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone	923:1070	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	1	51	theme	ECF	231:233	arg1	factors					242:248	extracytoplasmic function (ECF) sigma factors	204:248	extracytoplasmic function (ECF) sigma factors	204:248	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	0	52	theme	sigma	40:44	arg1	factors					46:52	extracytoplasmic function sigma factors	14:52	extracytoplasmic function sigma factors	14:52	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	5	53	theme	PG	1086:1087	arg1	content					1089:1095	PG content	1086:1095	PG content (by repressing Pspac-pgsA)	1086:1122	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	0	54	from	glucolipids	70:80	arg1	cells					149:153	Bacillus subtilis cells	131:153	Bacillus subtilis cells lacking lipoteichoic acid	131:179	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	2	55	theme	glycerophosphate	422:437	arg1	moiety					439:444	the glycerophosphate moiety	418:444	the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis,	418:525	LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane.					
30971625	6	56	theme	regulation	1413:1422	arg1	systems					1424:1430	the regulation systems	1409:1430	the regulation systems of some ECF sigma factors	1409:1456	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	2	57	theme	major	494:498	arg1	glucolipid					500:509	a major glucolipid	492:509	a major glucolipid in B. subtilis	492:524	LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane.					
30971625	2	57	theme	major	494:498	arg1	diglucosyldiacylglycerol					457:480	diglucosyldiacylglycerol	457:480	diglucosyldiacylglycerol (DGlcDG)	457:489	LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane.					
30971625	4	58	theme	σX	948:949	arg1	levels					934:939	activation levels	923:939	activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone	923:1070	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	1	59	theme	absence	315:321	arg1	reduction					267:275	reduction	267:275	reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA)	267:375	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	2	60	theme	PG	449:450	arg1	moiety					439:444	the glycerophosphate moiety	418:444	the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis,	418:525	LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane.					
30971625	3	61	theme	glucolipids	712:722	arg1	absence					701:707	absence	701:707	absence	701:707	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	3	61	theme	glucolipids	712:722	arg1	content					690:696	PG content	687:696	PG content	687:696	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	0	62	theme	lipoteichoic	163:174	arg1	acid					176:179	lipoteichoic acid	163:179	lipoteichoic acid	163:179	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	2	63	from	glucolipid	500:509	arg1	subtilis					517:524	B. subtilis	514:524	B. subtilis	514:524	LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane.					
30971625	5	64	from	Reduction	1073:1081	arg1	cells					1131:1135	the cells	1127:1135	the cells lacking LTA	1127:1147	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	3	65	theme	glucolipids	595:605	arg1	absence					584:590	absence	584:590	absence	584:590	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	3	65	theme	glucolipids	595:605	arg1	content					573:579	PG content	570:579	PG content	570:579	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	1	66	theme	glucolipids	326:336	arg1	absence					342:348	absence	342:348	absence of lipoteichoic acid (LTA)	342:375	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	1	66	theme	glucolipids	326:336	arg1	absence					315:321	absence	315:321	absence of glucolipids	315:336	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	1	66	theme	glucolipids	326:336	arg1	content					306:312	content	306:312	content	306:312	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	1	66	theme	glucolipids	326:336	arg1	PG					302:303	PG	302:303	PG	302:303	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	1	66	theme	glucolipids	326:336	arg1	phosphatidylglycerol					280:299	phosphatidylglycerol	280:299	phosphatidylglycerol	280:299	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	5	67	dep	σM	1201:1202	arg1	1.9-					1222:1225	1.9-	1222:1225	1.9-	1222:1225	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	5	67	dep	σM	1201:1202	arg1	2.3-					1216:1219	2.3-	1216:1219	2.3-	1216:1219	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	5	67	dep	σM	1201:1202	arg1	2.2-fold					1231:1238	2.2-fold	1231:1238	2.2-fold	1231:1238	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	3	68	theme	absence	584:590	arg1	reduction					557:565	reduction	557:565	reduction of PG content or absence of glucolipids	557:605	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	3	69	theme	PG	570:571	arg1	content					573:579	PG content	570:579	PG content	570:579	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	6	70	theme	PG	1324:1325	arg1	glucolipids					1296:1306	glucolipids	1296:1306	glucolipids	1296:1306	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	6	70	theme	PG	1324:1325	arg1	reduction					1311:1319	reduction	1311:1319	reduction of PG	1311:1325	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	1	71	theme	sigma	236:240	arg1	factors					242:248	extracytoplasmic function (ECF) sigma factors	204:248	extracytoplasmic function (ECF) sigma factors	204:248	In Bacillus subtilis, extracytoplasmic function (ECF) sigma factors are activated by reduction of phosphatidylglycerol (PG) content, absence of glucolipids, or absence of lipoteichoic acid (LTA).					
30971625	2	72	theme	plasma	534:539	arg1	membrane					541:548	the plasma membrane	530:548	the plasma membrane	530:548	LTA is synthesized by polymerization of the glycerophosphate moiety of PG onto diglucosyldiacylglycerol (DGlcDG), a major glucolipid in B. subtilis, in the plasma membrane.					
30971625	5	73	theme	σW	1205:1206	arg1	levels					1191:1196	activation levels	1180:1196	activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively)	1180:1253	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	0	74	from	cells	149:153	arg1	removal					59:65	removal	59:65	removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid	59:179	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	0	75	theme	glucolipids	70:80	arg1	removal					59:65	removal	59:65	removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid	59:179	Activation of extracytoplasmic function sigma factors upon removal of glucolipids and reduction of phosphatidylglycerol content in Bacillus subtilis cells lacking lipoteichoic acid.					
30971625	6	76	theme	plasma	1465:1470	arg1	membrane					1472:1479	the plasma membrane	1461:1479	the plasma membrane	1461:1479	These results suggested that absence of glucolipids or reduction of PG alone, not the possible secondary alteration in LTA, leads to changes that affect the regulation systems of some ECF sigma factors in the plasma membrane.					
30971625	3	77	theme	content	573:579	arg1	reduction					557:565	reduction	557:565	reduction of PG content or absence of glucolipids	557:605	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	5	78	theme	σV	1212:1213	arg1	levels					1191:1196	activation levels	1180:1196	activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively)	1180:1253	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	4	79	theme	responsible	833:843	arg1	ugtP					827:830	ugtP	827:830	ugtP	827:830	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	5	80	theme	additive	1159:1166	arg1	increase					1168:1175	an additive increase	1156:1175	an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively)	1156:1253	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	5	81	theme	σM	1201:1202	arg1	levels					1191:1196	activation levels	1180:1196	activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively)	1180:1253	Reduction of PG content (by repressing Pspac-pgsA) in the cells lacking LTA caused an additive increase of activation levels of σM, σW and σV (2.3-, 1.9- and 2.2-fold, respectively).					
30971625	4	82	from	levels	1038:1043	arg1	cells					1048:1052	cells	1048:1052	cells lacking LTA alone	1048:1070	Disruption of ugtP, responsible for glucolipid synthesis, in cells lacking LTA caused an additive increase of activation levels of σM, σX, σV and σY (3.1-, 2.2-, 2.1- and 1.4-fold, respectively), relative to their activation levels in cells lacking LTA alone.					
30971625	3	83	theme	sigma	754:758	arg1	factors					760:766	ECF sigma factors	750:766	ECF sigma factors	750:766	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
30971625	3	84	theme	ECF	750:752	arg1	factors					760:766	ECF sigma factors	750:766	ECF sigma factors	750:766	Thus, reduction of PG content or absence of glucolipids might cause some changes in LTA, and hence we investigated whether reduction of PG content or absence of glucolipids induces the activation of ECF sigma factors independently from an ensuing change in LTA.					
31087770	3	0	theme	biological	655:664	arg1	performance					666:676	biological performance	655:676	biological performance	655:676	It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio.					
31087770	8	1	theme	compressive	1396:1406	arg1	stress					1408:1413	a maximum compressive stress	1386:1413	a maximum compressive stress of 0.26 MPa	1386:1425	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	4	2	theme	PRP	854:856	arg1	addition					842:849	the addition	838:849	the addition of PRP, which produced also a more homogeneous gel structure	838:910	The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure.					
31087770	12	3	theme	tissue	2045:2050	arg1	engineering					2052:2062	cartilage tissue engineering	2035:2062	cartilage tissue engineering	2035:2062	Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.					
31087770	3	4	theme	composite	685:693	arg1	hydrogels					695:703	the composite hydrogels	681:703	the composite hydrogels	681:703	It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio.					
31087770	6	5	theme	PRP	1203:1205	arg1	addition					1191:1198	the addition	1187:1198	the addition of PRP	1187:1205	On the other hand, an increase in surface roughness was observed after the addition of PRP.					
31087770	9	6	theme	in	1575:1576	arg1	degradation					1584:1594	the in vitro degradation	1571:1594	the in vitro degradation of the alginate gel	1571:1614	It was also found that the in vitro degradation of the alginate gel was accelerated by the addition of PRP.					
31087770	1	7	theme	cell	195:198	arg1	scaffolds					151:159	Three-dimensional scaffolds	133:159	Three-dimensional scaffolds like hydrogels	133:174	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	1	7	theme	cell	195:198	arg1	carriers					200:207	cell carriers	195:207	cell carriers for in vitro or in vivo colonization	195:244	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	12	8	theme	cell	2017:2020	arg1	carriers					2022:2029	cell carriers	2017:2029	cell carriers for cartilage tissue engineering	2017:2062	Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.					
31087770	0	9	theme	cartilage	115:123	arg1	repair					125:130	cartilage repair	115:130	cartilage repair	115:130	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	11	10	theme	composite	1751:1759	arg1	hydrogels					1761:1769	the composite hydrogels	1747:1769	the composite hydrogels	1747:1769	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	4	11	theme	gel	898:900	arg1	structure					902:910	a more homogeneous gel structure	879:910	a more homogeneous gel structure	879:910	The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure.					
31087770	9	12	theme	gel	1612:1614	arg1	degradation					1584:1594	the in vitro degradation	1571:1594	the in vitro degradation of the alginate gel	1571:1614	It was also found that the in vitro degradation of the alginate gel was accelerated by the addition of PRP.					
31087770	1	13	dep	in	225:226	arg1	vivo					228:231	vivo	228:231	vivo	228:231	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	12	14	theme	SA/PRP	1987:1992	arg1	hydrogels					2004:2012	SA/PRP composite hydrogels	1987:2012	SA/PRP composite hydrogels	1987:2012	Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.					
31087770	3	15	theme	blending	733:740	arg1	ratio					742:746	the blending ratio	729:746	the blending ratio	729:746	It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio.					
31087770	5	16	theme	proteins	1023:1030	arg1	incorporation					994:1006	the incorporation	990:1006	the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found	990:1113	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	10	17	theme	cellular	1668:1675	arg1	responses					1677:1685	cellular responses	1668:1685	cellular responses	1668:1685	In terms of cellular responses, all gels exhibited an excellent cytocompatibility.					
31087770	11	18	theme	genes	1905:1909	arg1	up-regulation					1868:1880	up-regulation	1868:1880	up-regulation of chondrogenic marker genes Sox9 and Aggrecan	1868:1927	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	7	19	theme	pure	1307:1310	arg1	gel					1315:1317	the pure SA gel	1303:1317	the pure SA gel	1303:1317	The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel.					
31087770	12	20	theme	hydrogels	2004:2012	arg1	potential					1974:1982	a great potential	1966:1982	a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering	1966:2062	Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.					
31087770	8	21	from	stress	1528:1533	arg1	%					1521:1521	only 45%	1514:1521	only 45%	1514:1521	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	8	21	from	stress	1528:1533	arg1	strain					1482:1487	the maximum compressive strain	1458:1487	the maximum compressive strain of pure SA hydrogels	1458:1508	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	1	22	theme	Three-dimensional	133:149	arg1	carriers					200:207	cell carriers	195:207	cell carriers for in vitro or in vivo colonization	195:244	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	1	22	theme	Three-dimensional	133:149	arg1	scaffolds					151:159	Three-dimensional scaffolds	133:159	Three-dimensional scaffolds like hydrogels	133:174	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	0	23	theme	composite	64:72	arg1	carrier					103:109	a potential cell carrier	86:109	a potential cell carrier for cartilage repair	86:130	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	0	23	theme	composite	64:72	arg1	hydrogel					74:81	composite hydrogel	64:81	composite hydrogel	64:81	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	11	24	theme	marker	1898:1903	arg1	genes					1905:1909	chondrogenic marker genes Sox9 and Aggrecan	1885:1927	chondrogenic marker genes Sox9 and Aggrecan	1885:1927	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	24	theme	marker	1898:1903	arg1	Aggrecan					1920:1927	Aggrecan	1920:1927	Aggrecan	1920:1927	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	24	theme	marker	1898:1903	arg1	Sox9					1911:1914	Sox9	1911:1914	Sox9	1911:1914	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	6	25	from	increase	1138:1145	arg1	roughness					1158:1166	surface roughness	1150:1166	surface roughness	1150:1166	On the other hand, an increase in surface roughness was observed after the addition of PRP.					
31087770	5	26	theme	200-300 μm	1094:1103	arg1	size					1086:1089	a pore size	1079:1089	a pore size of 200-300 μm	1079:1103	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	6	27	theme	surface	1150:1156	arg1	roughness					1158:1166	surface roughness	1150:1166	surface roughness	1150:1166	On the other hand, an increase in surface roughness was observed after the addition of PRP.					
31087770	0	28	theme	potential	88:96	arg1	carrier					103:109	a potential cell carrier	86:109	a potential cell carrier for cartilage repair	86:130	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	0	28	theme	potential	88:96	arg1	hydrogel					74:81	composite hydrogel	64:81	composite hydrogel	64:81	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	5	29	theme	electron	937:944	arg1	FE-SEM					958:963	FE-SEM	958:963	FE-SEM	958:963	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	5	29	theme	electron	937:944	arg1	microscopy					946:955	Field emission scanning electron microscopy	913:955	Field emission scanning electron microscopy (FE-SEM) investigation	913:978	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	2	30	theme	sodium	373:378	arg1	SA					390:391	SA	390:391	SA	390:391	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	2	30	theme	sodium	373:378	arg1	alginate					380:387	sodium alginate	373:387	sodium alginate (SA)	373:392	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	0	31	theme	injectable	33:42	arg1	sodium					44:49	an injectable sodium	30:49	an injectable sodium	30:49	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	1	32	theme	major	264:268	arg1	topic					279:283	a major research topic	262:283	a major research topic to replace damaged tissue	262:309	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	8	33	theme	compressive	1470:1480	arg1	strain					1482:1487	the maximum compressive strain	1458:1487	the maximum compressive strain of pure SA hydrogels	1458:1508	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	8	33	theme	compressive	1470:1480	arg1	%					1521:1521	only 45%	1514:1521	only 45%	1514:1521	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	8	34	theme	composite	1324:1332	arg1	hydrogels					1334:1342	The composite hydrogels	1320:1342	The composite hydrogels with the highest PRP content	1320:1371	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	5	35	theme	emission	919:926	arg1	FE-SEM					958:963	FE-SEM	958:963	FE-SEM	958:963	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	5	35	theme	emission	919:926	arg1	microscopy					946:955	Field emission scanning electron microscopy	913:955	Field emission scanning electron microscopy (FE-SEM) investigation	913:978	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	7	36	theme	hydrogel	1264:1271	arg1	strength					1235:1242	The compressive mechanical strength	1208:1242	The compressive mechanical strength of SA/PRP composite hydrogel	1208:1271	The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel.					
31087770	2	37	theme	blending	436:443	arg1	ratios					445:450	blending ratios	436:450	blending ratios	436:450	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	7	38	theme	SA/PRP	1247:1252	arg1	hydrogel					1264:1271	SA/PRP composite hydrogel	1247:1271	SA/PRP composite hydrogel	1247:1271	The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel.					
31087770	8	39	theme	SA	1497:1498	arg1	hydrogels					1500:1508	pure SA hydrogels	1492:1508	pure SA hydrogels	1492:1508	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	3	40	theme	PRP	607:609	arg1	addition					595:602	addition	595:602	addition of PRP	595:609	It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio.					
31087770	8	41	theme	highest	1353:1359	arg1	content					1365:1371	the highest PRP content	1349:1371	the highest PRP content	1349:1371	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	0	42	theme	sodium	44:49	arg1	properties					16:25	properties	16:25	properties	16:25	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	0	42	theme	sodium	44:49	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	5	43	theme	PRP-derived	1011:1021	arg1	proteins					1023:1030	PRP-derived proteins	1011:1030	PRP-derived proteins	1011:1030	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	6	44	located	observed	1172:1179	arg1	hand					1129:1132	the other hand	1119:1132	the other hand	1119:1132	On the other hand, an increase in surface roughness was observed after the addition of PRP.					
31087770	6	44	located	observed	1172:1179	arg2	increase					1138:1145	an increase	1135:1145	an increase in surface roughness	1135:1166	On the other hand, an increase in surface roughness was observed after the addition of PRP.					
31087770	1	45	dep	in	213:214	arg1	vitro					216:220	vitro	216:220	vitro	216:220	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	7	46	theme	compressive	1212:1222	arg1	strength					1235:1242	The compressive mechanical strength	1208:1242	The compressive mechanical strength of SA/PRP composite hydrogel	1208:1271	The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel.					
31087770	3	47	theme	physical	631:638	arg1	properties					640:649	the physical properties	627:649	the physical properties	627:649	It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio.					
31087770	4	48	theme	swelling	771:778	arg1	ratio					780:784	swelling ratio	771:784	swelling ratio	771:784	The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure.					
31087770	2	49	theme	composite	342:350	arg1	hydrogel					352:359	a novel composite hydrogel	334:359	a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL)	334:548	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	12	50	theme	present	1943:1949	arg1	study					1951:1955	the present study	1939:1955	the present study	1939:1955	Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.					
31087770	4	51	theme	hydrogels	798:806	arg1	rate					762:765	gelation rate	753:765	gelation rate	753:765	The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure.					
31087770	4	51	theme	hydrogels	798:806	arg1	ratio					780:784	swelling ratio	771:784	swelling ratio	771:784	The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure.					
31087770	8	52	theme	0.08 MPa	1538:1545	arg1	stress					1528:1533	a stress	1526:1533	a stress of 0.08 MPa	1526:1545	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	11	53	link	marrow-derived	1786:1799	arg1	proliferation					1824:1836	proliferation	1824:1836	proliferation	1824:1836	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	53	link	marrow-derived	1786:1799	arg1	chondrogenesis					1848:1861	their chondrogenesis	1842:1861	their chondrogenesis	1842:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	53	link	marrow-derived	1786:1799	arg1	cells					1818:1822	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	6	54	theme	other	1123:1127	arg1	hand					1129:1132	the other hand	1119:1132	the other hand	1119:1132	On the other hand, an increase in surface roughness was observed after the addition of PRP.					
31087770	12	55	theme	cartilage	2035:2043	arg1	engineering					2052:2062	cartilage tissue engineering	2035:2062	cartilage tissue engineering	2035:2062	Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.					
31087770	3	56	theme	hydrogels	695:703	arg1	properties					640:649	the physical properties	627:649	the physical properties	627:649	It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio.					
31087770	3	56	theme	hydrogels	695:703	arg1	performance					666:676	biological performance	655:676	biological performance	655:676	It was found that addition of PRP changed largely the physical properties and biological performance of the composite hydrogels, which was depending on the blending ratio.					
31087770	4	57	dep	rate	762:765	arg1	The					749:751	The	749:751	The	749:751	The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure.					
31087770	8	58	theme	maximum	1388:1394	arg1	stress					1408:1413	a maximum compressive stress	1386:1413	a maximum compressive stress of 0.26 MPa	1386:1425	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	8	59	with	hydrogels	1334:1342	arg1	content					1365:1371	the highest PRP content	1349:1371	the highest PRP content	1349:1371	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	5	60	from	incorporation	994:1006	arg1	hydrogel					1039:1046	the hydrogel	1035:1046	the hydrogel	1035:1046	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	1	61	theme	in	213:214	arg1	colonization					233:244	in vitro or in vivo colonization	213:244	in vitro or in vivo colonization	213:244	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	5	62	link	PRP-derived	1011:1021	arg1	proteins					1023:1030	PRP-derived proteins	1011:1030	PRP-derived proteins	1011:1030	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	9	63	theme	alginate	1603:1610	arg1	gel					1612:1614	the alginate gel	1599:1614	the alginate gel	1599:1614	It was also found that the in vitro degradation of the alginate gel was accelerated by the addition of PRP.					
31087770	5	64	theme	porous	1057:1062	arg1	structure					1064:1072	a porous structure	1055:1072	a porous structure with a pore size of 200-300 μm	1055:1103	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	10	65	theme	responses	1677:1685	arg1	terms					1659:1663	terms	1659:1663	terms of cellular responses	1659:1685	In terms of cellular responses, all gels exhibited an excellent cytocompatibility.					
31087770	2	66	attach	released	485:492	arg1	carbonate-D-Glucono-d-lactone					507:535	calcium carbonate-D-Glucono-d-lactone	499:535	calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL)	499:548	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	2	66	attach	released	485:492	arg2	ratios					445:450	blending ratios	436:450	blending ratios	436:450	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	2	66	attach	released	485:492	arg1	-GDL					544:547	CaCO3 -GDL	538:547	CaCO3 -GDL	538:547	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	8	67	theme	0.26 MPa	1418:1425	arg1	stress					1408:1413	a maximum compressive stress	1386:1413	a maximum compressive stress of 0.26 MPa	1386:1425	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	10	68	theme	excellent	1710:1718	arg1	cytocompatibility					1720:1736	an excellent cytocompatibility	1707:1736	an excellent cytocompatibility	1707:1736	In terms of cellular responses, all gels exhibited an excellent cytocompatibility.					
31087770	2	69	theme	CaCO3	538:542	arg1	carbonate-D-Glucono-d-lactone					507:535	calcium carbonate-D-Glucono-d-lactone	499:535	calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL)	499:548	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	2	69	theme	CaCO3	538:542	arg1	-GDL					544:547	CaCO3 -GDL	538:547	CaCO3 -GDL	538:547	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	8	70	theme	maximum	1429:1435	arg1	strain					1437:1442	a maximum strain	1427:1442	a maximum strain of 55%	1427:1449	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	7	71	theme	SA	1312:1313	arg1	gel					1315:1317	the pure SA gel	1303:1317	the pure SA gel	1303:1317	The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel.					
31087770	12	72	theme	composite	1994:2002	arg1	hydrogels					2004:2012	SA/PRP composite hydrogels	1987:2012	SA/PRP composite hydrogels	1987:2012	Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.					
31087770	5	73	with	structure	1064:1072	arg1	size					1086:1089	a pore size	1079:1089	a pore size of 200-300 μm	1079:1103	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	11	74	theme	chondrogenic	1885:1896	arg1	genes					1905:1909	chondrogenic marker genes Sox9 and Aggrecan	1885:1927	chondrogenic marker genes Sox9 and Aggrecan	1885:1927	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	74	theme	chondrogenic	1885:1896	arg1	Aggrecan					1920:1927	Aggrecan	1920:1927	Aggrecan	1920:1927	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	74	theme	chondrogenic	1885:1896	arg1	Sox9					1911:1914	Sox9	1911:1914	Sox9	1911:1914	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	9	75	theme	PRP	1651:1653	arg1	addition					1639:1646	the addition	1635:1646	the addition of PRP	1635:1653	It was also found that the in vitro degradation of the alginate gel was accelerated by the addition of PRP.					
31087770	8	76	theme	%	1449:1449	arg1	strain					1437:1442	a maximum strain	1427:1442	a maximum strain of 55%	1427:1449	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	12	77	theme	great	1968:1972	arg1	potential					1974:1982	a great potential	1966:1982	a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering	1966:2062	Overall, the present study suggests a great potential of SA/PRP composite hydrogels as cell carriers for cartilage tissue engineering.					
31087770	0	78	theme	cell	98:101	arg1	carrier					103:109	a potential cell carrier	86:109	a potential cell carrier for cartilage repair	86:130	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	0	78	theme	cell	98:101	arg1	hydrogel					74:81	composite hydrogel	64:81	composite hydrogel	64:81	Fabrication and properties of an injectable sodium alginate/PRP composite hydrogel as a potential cell carrier for cartilage repair.					
31087770	8	79	theme	PRP	1361:1363	arg1	content					1365:1371	the highest PRP content	1349:1371	the highest PRP content	1349:1371	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	5	80	theme	scanning	928:935	arg1	FE-SEM					958:963	FE-SEM	958:963	FE-SEM	958:963	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	5	80	theme	scanning	928:935	arg1	microscopy					946:955	Field emission scanning electron microscopy	913:955	Field emission scanning electron microscopy (FE-SEM) investigation	913:978	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	5	81	theme	microscopy	946:955	arg1	investigation					966:978	Field emission scanning electron microscopy (FE-SEM) investigation	913:978	Field emission scanning electron microscopy (FE-SEM) investigation	913:978	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	2	82	theme	calcium	471:477	arg1	ions					479:482	calcium ions	471:482	calcium ions	471:482	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	8	83	theme	maximum	1462:1468	arg1	strain					1482:1487	the maximum compressive strain	1458:1487	the maximum compressive strain of pure SA hydrogels	1458:1508	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	8	83	theme	maximum	1462:1468	arg1	%					1521:1521	only 45%	1514:1521	only 45%	1514:1521	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	11	84	theme	stem	1813:1816	arg1	proliferation					1824:1836	proliferation	1824:1836	proliferation	1824:1836	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	84	theme	stem	1813:1816	arg1	chondrogenesis					1848:1861	their chondrogenesis	1842:1861	their chondrogenesis	1842:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	84	theme	stem	1813:1816	arg1	cells					1818:1822	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	5	85	theme	pore	1081:1084	arg1	size					1086:1089	a pore size	1079:1089	a pore size of 200-300 μm	1079:1103	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	2	86	theme	calcium	499:505	arg1	carbonate-D-Glucono-d-lactone					507:535	calcium carbonate-D-Glucono-d-lactone	499:535	calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL)	499:548	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	2	86	theme	calcium	499:505	arg1	-GDL					544:547	CaCO3 -GDL	538:547	CaCO3 -GDL	538:547	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	11	87	theme	marrow-derived	1786:1799	arg1	proliferation					1824:1836	proliferation	1824:1836	proliferation	1824:1836	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	87	theme	marrow-derived	1786:1799	arg1	chondrogenesis					1848:1861	their chondrogenesis	1842:1861	their chondrogenesis	1842:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	87	theme	marrow-derived	1786:1799	arg1	cells					1818:1822	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	1	88	theme	research	270:277	arg1	topic					279:283	a major research topic	262:283	a major research topic to replace damaged tissue	262:309	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	5	89	theme	Field	913:917	arg1	FE-SEM					958:963	FE-SEM	958:963	FE-SEM	958:963	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	5	89	theme	Field	913:917	arg1	microscopy					946:955	Field emission scanning electron microscopy	913:955	Field emission scanning electron microscopy (FE-SEM) investigation	913:978	Field emission scanning electron microscopy (FE-SEM) investigation confirmed the incorporation of PRP-derived proteins in the hydrogel, where a porous structure with a pore size of 200-300 μm was found.					
31087770	7	90	theme	composite	1254:1262	arg1	hydrogel					1264:1271	SA/PRP composite hydrogel	1247:1271	SA/PRP composite hydrogel	1247:1271	The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel.					
31087770	8	91	theme	pure	1492:1495	arg1	hydrogels					1500:1508	pure SA hydrogels	1492:1508	pure SA hydrogels	1492:1508	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	9	92	dep	in	1575:1576	arg1	vitro					1578:1582	vitro	1578:1582	vitro	1578:1582	It was also found that the in vitro degradation of the alginate gel was accelerated by the addition of PRP.					
31087770	11	93	dep	genes	1905:1909	arg1	genes					1905:1909	chondrogenic marker genes Sox9 and Aggrecan	1885:1927	chondrogenic marker genes Sox9 and Aggrecan	1885:1927	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	93	dep	genes	1905:1909	arg1	Aggrecan					1920:1927	Aggrecan	1920:1927	Aggrecan	1920:1927	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	93	dep	genes	1905:1909	arg1	Sox9					1911:1914	Sox9	1911:1914	Sox9	1911:1914	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	94	theme	mesenchymal	1801:1811	arg1	proliferation					1824:1836	proliferation	1824:1836	proliferation	1824:1836	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	94	theme	mesenchymal	1801:1811	arg1	chondrogenesis					1848:1861	their chondrogenesis	1842:1861	their chondrogenesis	1842:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	94	theme	mesenchymal	1801:1811	arg1	cells					1818:1822	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	8	95	theme	hydrogels	1500:1508	arg1	strain					1482:1487	the maximum compressive strain	1458:1487	the maximum compressive strain of pure SA hydrogels	1458:1508	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	8	95	theme	hydrogels	1500:1508	arg1	%					1521:1521	only 45%	1514:1521	only 45%	1514:1521	The composite hydrogels with the highest PRP content exhibited at a maximum compressive stress of 0.26 MPa a maximum strain of 55%, while the maximum compressive strain of pure SA hydrogels was only 45% at a stress of 0.08 MPa.					
31087770	1	96	theme	damaged	296:302	arg1	tissue					304:309	damaged tissue	296:309	damaged tissue	296:309	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	7	97	theme	mechanical	1224:1233	arg1	strength					1235:1242	The compressive mechanical strength	1208:1242	The compressive mechanical strength of SA/PRP composite hydrogel	1208:1271	The compressive mechanical strength of SA/PRP composite hydrogel was enhanced in comparison to the pure SA gel.					
31087770	4	98	theme	homogeneous	886:896	arg1	structure					902:910	a more homogeneous gel structure	879:910	a more homogeneous gel structure	879:910	The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure.					
31087770	4	99	theme	alginate	789:796	arg1	hydrogels					798:806	alginate hydrogels	789:806	alginate hydrogels	789:806	The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure.					
31087770	2	100	theme	current	319:325	arg1	study					327:331	the current study	315:331	the current study	315:331	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31087770	11	101	dep	cells	1818:1822	arg1	proliferation					1824:1836	proliferation	1824:1836	proliferation	1824:1836	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	101	dep	cells	1818:1822	arg1	chondrogenesis					1848:1861	their chondrogenesis	1842:1861	their chondrogenesis	1842:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	11	101	dep	cells	1818:1822	arg1	cells					1818:1822	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis	1781:1861	Indeed, the composite hydrogels supported bone marrow-derived mesenchymal stem cells proliferation and their chondrogenesis with up-regulation of chondrogenic marker genes Sox9 and Aggrecan.					
31087770	4	102	theme	gelation	753:760	arg1	rate					762:765	gelation rate	753:765	gelation rate	753:765	The gelation rate and swelling ratio of alginate hydrogels were significantly reduced by the addition of PRP, which produced also a more homogeneous gel structure.					
31087770	1	103	theme	in	225:226	arg1	colonization					233:244	in vitro or in vivo colonization	213:244	in vitro or in vivo colonization	213:244	Three-dimensional scaffolds like hydrogels can be employed as cell carriers for in vitro or in vivo colonization and have become a major research topic to replace damaged tissue.					
31087770	2	104	theme	novel	336:340	arg1	hydrogel					352:359	a novel composite hydrogel	334:359	a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL)	334:548	In the current study, a novel composite hydrogel composed of sodium alginate (SA) and platelet-rich-plasma (PRP) varying in blending ratios, cross-linked with calcium ions, released from calcium carbonate-D-Glucono-d-lactone (CaCO3 -GDL) was successfully prepared.					
31053693	5	0	theme	scaffolds	759:767	arg1	Bioactivity					725:735	The Bioactivity	721:735	The Bioactivity of the Nano composite scaffolds	721:767	The Bioactivity of the Nano composite scaffolds was studied using simulated body fluid solution at 37 ± 1°C.					
31053693	1	1	with	gelatin	248:254	arg1	size					277:280	an average pore size	261:280	an average pore size of 250-1010 nm	261:295	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	1	2	theme	composite	182:190	arg1	scaffold					192:199	porous composite scaffold	175:199	porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method	175:365	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	1	3	theme	average	264:270	arg1	size					277:280	an average pore size	261:280	an average pore size of 250-1010 nm	261:295	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	8	4	theme	Cytocompatibility	1174:1190	arg1	evaluation					1192:1201	Cytocompatibility evaluation	1174:1201	Cytocompatibility evaluation for Vero Cells using wound scratch assay	1174:1242	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	6	5	theme	[nano	927:931	arg1	gelatin					957:963	nano Chitosan gelatin	943:963	nano Chitosan gelatin	943:963	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	5	theme	[nano	927:931	arg1	gelatin					1001:1007	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	5	theme	[nano	927:931	arg1	Chitosan					933:940	the prepared nanocomposites [nano Chitosan	899:940	the prepared nanocomposites [nano Chitosan	899:940	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	8	6	theme	fabricated	1260:1269	arg1	scaffold					1292:1299	the fabricated porous nanocomposite scaffold	1256:1299	the fabricated porous nanocomposite scaffold	1256:1299	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	1	7	theme	scaffold	192:199	arg1	characterisation					155:170	characterisation	155:170	characterisation	155:170	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	1	7	theme	scaffold	192:199	arg1	fabrication					139:149	fabrication	139:149	fabrication	139:149	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	1	8	theme	pore	272:275	arg1	size					277:280	an average pore size	261:280	an average pore size of 250-1010 nm	261:295	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	8	9	theme	Vero	1207:1210	arg1	Cells					1212:1216	Vero Cells	1207:1216	Vero Cells	1207:1216	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	6	10	theme	nanocomposites	912:925	arg1	gelatin					957:963	nano Chitosan gelatin	943:963	nano Chitosan gelatin	943:963	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	10	theme	nanocomposites	912:925	arg1	gelatin					1001:1007	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	10	theme	nanocomposites	912:925	arg1	Chitosan					933:940	the prepared nanocomposites [nano Chitosan	899:940	the prepared nanocomposites [nano Chitosan	899:940	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	3	11	theme	Chitosan	539:546	arg1	bonds					518:522	functional group bonds	501:522	functional group bonds of HAP powder, Chitosan and scaffold	501:559	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	4	12	theme	scaffold	657:664	arg1	morphology					639:648	The surface morphology	627:648	The surface morphology of the scaffold	627:664	The surface morphology of the scaffold was observed using Scanning Electron Microscope (SEM).					
31053693	6	13	theme	Chitosan	992:999	arg1	gelatin					1001:1007	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	13	theme	Chitosan	992:999	arg1	Chitosan					933:940	the prepared nanocomposites [nano Chitosan	899:940	the prepared nanocomposites [nano Chitosan	899:940	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	14	theme	prepared	903:910	arg1	gelatin					957:963	nano Chitosan gelatin	943:963	nano Chitosan gelatin	943:963	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	14	theme	prepared	903:910	arg1	gelatin					1001:1007	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	14	theme	prepared	903:910	arg1	Chitosan					933:940	the prepared nanocomposites [nano Chitosan	899:940	the prepared nanocomposites [nano Chitosan	899:940	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	3	15	theme	group	512:516	arg1	bonds					518:522	functional group bonds	501:522	functional group bonds of HAP powder, Chitosan and scaffold	501:559	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	8	16	theme	scratch	1230:1236	arg1	assay					1238:1242	wound scratch assay	1224:1242	wound scratch assay	1224:1242	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	4	17	theme	Electron	694:701	arg1	SEM					715:717	SEM	715:717	SEM	715:717	The surface morphology of the scaffold was observed using Scanning Electron Microscope (SEM).					
31053693	4	17	theme	Electron	694:701	arg1	Microscope					703:712	Scanning Electron Microscope	685:712	Scanning Electron Microscope (SEM)	685:718	The surface morphology of the scaffold was observed using Scanning Electron Microscope (SEM).					
31053693	8	18	theme	cell	1316:1319	arg1	proliferation					1321:1333	cell proliferation	1316:1333	cell proliferation	1316:1333	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	3	19	theme	powder	531:536	arg1	bonds					518:522	functional group bonds	501:522	functional group bonds of HAP powder, Chitosan and scaffold	501:559	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	7	20	dep	migration	1020:1028	arg1	The					1011:1013	The	1011:1013	The	1011:1013	The cell migration and potential biocompatibility of nHAP-chitosan-gelatin scaffold was assessed via wound scratch assay and were compared to povedeen as control.					
31053693	3	21	dep	Fourier	579:585	arg1	Transform					587:595	Transform	587:595	Transform Infrared Spectroscopy (FTIR)	587:624	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	5	22	theme	body	797:800	arg1	solution					808:815	simulated body fluid solution	787:815	simulated body fluid solution	787:815	The Bioactivity of the Nano composite scaffolds was studied using simulated body fluid solution at 37 ± 1°C.					
31053693	9	23	theme	damaged	1477:1483	arg1	tissue					1490:1495	damaged cell tissue	1477:1495	damaged cell tissue	1477:1495	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	0	24	theme	In	0:1	arg1	evaluation					9:18	In vitro evaluation	0:18	In vitro evaluation of biodegradable nHAP-Chitosan-Gelatin-based scaffold for tissue engineering application.	0:108	In vitro evaluation of biodegradable nHAP-Chitosan-Gelatin-based scaffold for tissue engineering application.					
31053693	8	25	theme	nanocomposite	1278:1290	arg1	scaffold					1292:1299	the fabricated porous nanocomposite scaffold	1256:1299	the fabricated porous nanocomposite scaffold	1256:1299	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	5	26	theme	fluid	802:806	arg1	solution					808:815	simulated body fluid solution	787:815	simulated body fluid solution	787:815	The Bioactivity of the Nano composite scaffolds was studied using simulated body fluid solution at 37 ± 1°C.					
31053693	6	27	theme	Nano	966:969	arg1	gelatin					1001:1007	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	27	theme	Nano	966:969	arg1	Chitosan					933:940	the prepared nanocomposites [nano Chitosan	899:940	the prepared nanocomposites [nano Chitosan	899:940	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	7	28	theme	scratch	1118:1124	arg1	assay					1126:1130	wound scratch assay	1112:1130	wound scratch assay	1112:1130	The cell migration and potential biocompatibility of nHAP-chitosan-gelatin scaffold was assessed via wound scratch assay and were compared to povedeen as control.					
31053693	5	29	theme	Nano	744:747	arg1	scaffolds					759:767	the Nano composite scaffolds	740:767	the Nano composite scaffolds	740:767	The Bioactivity of the Nano composite scaffolds was studied using simulated body fluid solution at 37 ± 1°C.					
31053693	8	30	dep	higher	1309:1314	arg1	proliferation					1321:1333	cell proliferation	1316:1333	cell proliferation	1316:1333	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	8	30	dep	higher	1309:1314	arg1	growth					1339:1344	growth	1339:1344	growth	1339:1344	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	2	31	theme	crystallographic	443:458	arg1	structure					460:468	crystallographic structure	443:468	crystallographic structure of HAP powder	443:482	From the results of X-Ray Diffraction (XRD) study, the peaks correspond to crystallographic structure of HAP powder.					
31053693	1	32	theme	250-1010 nm	285:295	arg1	size					277:280	an average pore size	261:280	an average pore size of 250-1010 nm	261:295	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	1	33	theme	present	114:120	arg1	study					122:126	The present study	110:126	The present study	110:126	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	2	34	theme	Diffraction	394:404	arg1	study					412:416	X-Ray Diffraction (XRD) study	388:416	X-Ray Diffraction (XRD) study	388:416	From the results of X-Ray Diffraction (XRD) study, the peaks correspond to crystallographic structure of HAP powder.					
31053693	6	35	theme	Hydroxyapatite	977:990	arg1	gelatin					1001:1007	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	35	theme	Hydroxyapatite	977:990	arg1	Chitosan					933:940	the prepared nanocomposites [nano Chitosan	899:940	the prepared nanocomposites [nano Chitosan	899:940	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	1	36	with	chitosan	234:241	arg1	size					277:280	an average pore size	261:280	an average pore size of 250-1010 nm	261:295	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	9	37	theme	wound	1500:1504	arg1	healing					1506:1512	wound healing	1500:1512	wound healing process	1500:1520	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	3	38	theme	scaffold	552:559	arg1	bonds					518:522	functional group bonds	501:522	functional group bonds of HAP powder, Chitosan and scaffold	501:559	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	4	39	theme	surface	631:637	arg1	morphology					639:648	The surface morphology	627:648	The surface morphology of the scaffold	627:664	The surface morphology of the scaffold was observed using Scanning Electron Microscope (SEM).					
31053693	6	40	theme	based	971:975	arg1	gelatin					1001:1007	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	Nano based Hydroxyapatite Chitosan gelatin]	966:1008	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	40	theme	based	971:975	arg1	Chitosan					933:940	the prepared nanocomposites [nano Chitosan	899:940	the prepared nanocomposites [nano Chitosan	899:940	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	7	41	theme	wound	1112:1116	arg1	assay					1126:1130	wound scratch assay	1112:1130	wound scratch assay	1112:1130	The cell migration and potential biocompatibility of nHAP-chitosan-gelatin scaffold was assessed via wound scratch assay and were compared to povedeen as control.					
31053693	6	42	theme	Tris-Buffer	874:884	arg1	solution					886:893	Tris-Buffer solution	874:893	Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin]	874:1008	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	3	43	theme	bonds	518:522	arg1	presence					489:496	The presence	485:496	The presence of functional group bonds of HAP powder, Chitosan and scaffold	485:559	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	4	44	theme	Scanning	685:692	arg1	SEM					715:717	SEM	715:717	SEM	715:717	The surface morphology of the scaffold was observed using Scanning Electron Microscope (SEM).					
31053693	4	44	theme	Scanning	685:692	arg1	Microscope					703:712	Scanning Electron Microscope	685:712	Scanning Electron Microscope (SEM)	685:718	The surface morphology of the scaffold was observed using Scanning Electron Microscope (SEM).					
31053693	0	45	theme	nHAP-Chitosan-Gelatin-based	37:63	arg1	scaffold					65:72	biodegradable nHAP-Chitosan-Gelatin-based scaffold	23:72	biodegradable nHAP-Chitosan-Gelatin-based scaffold	23:72	In vitro evaluation of biodegradable nHAP-Chitosan-Gelatin-based scaffold for tissue engineering application.					
31053693	1	46	theme	wound	311:315	arg1	repair					317:322	wound repair	311:322	wound repair	311:322	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	3	47	theme	Infrared	597:604	arg1	FTIR					620:623	FTIR	620:623	FTIR	620:623	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	3	47	theme	Infrared	597:604	arg1	Spectroscopy					606:617	Infrared Spectroscopy	597:617	Infrared Spectroscopy (FTIR)	597:624	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	0	48	theme	biodegradable	23:35	arg1	scaffold					65:72	biodegradable nHAP-Chitosan-Gelatin-based scaffold	23:72	biodegradable nHAP-Chitosan-Gelatin-based scaffold	23:72	In vitro evaluation of biodegradable nHAP-Chitosan-Gelatin-based scaffold for tissue engineering application.					
31053693	9	49	theme	developed	1401:1409	arg1	scaffolds					1425:1433	the developed nanocomposite scaffolds	1397:1433	the developed nanocomposite scaffolds	1397:1433	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	9	49	theme	developed	1401:1409	arg1	candidates					1449:1458	potential candidates	1439:1458	potential candidates for regenerating damaged cell tissue in wound healing process	1439:1520	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	2	50	theme	powder	477:482	arg1	structure					460:468	crystallographic structure	443:468	crystallographic structure of HAP powder	443:482	From the results of X-Ray Diffraction (XRD) study, the peaks correspond to crystallographic structure of HAP powder.					
31053693	2	51	theme	study	412:416	arg1	results					377:383	the results	373:383	the results of X-Ray Diffraction (XRD) study	373:416	From the results of X-Ray Diffraction (XRD) study, the peaks correspond to crystallographic structure of HAP powder.					
31053693	2	52	theme	HAP	473:475	arg1	powder					477:482	HAP powder	473:482	HAP powder	473:482	From the results of X-Ray Diffraction (XRD) study, the peaks correspond to crystallographic structure of HAP powder.					
31053693	9	53	theme	nanocomposite	1411:1423	arg1	scaffolds					1425:1433	the developed nanocomposite scaffolds	1397:1433	the developed nanocomposite scaffolds	1397:1433	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	9	53	theme	nanocomposite	1411:1423	arg1	candidates					1449:1458	potential candidates	1439:1458	potential candidates for regenerating damaged cell tissue in wound healing process	1439:1520	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	7	54	theme	nHAP-chitosan-gelatin	1064:1084	arg1	scaffold					1086:1093	nHAP-chitosan-gelatin scaffold	1064:1093	nHAP-chitosan-gelatin scaffold	1064:1093	The cell migration and potential biocompatibility of nHAP-chitosan-gelatin scaffold was assessed via wound scratch assay and were compared to povedeen as control.					
31053693	1	55	contain	containing	201:210	arg2	chitosan					234:241	chitosan	234:241	chitosan	234:241	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	1	55	contain	containing	201:210	arg2	HAP					228:230	HAP	228:230	HAP	228:230	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	1	55	contain	containing	201:210	arg2	hydroxyapatite					212:225	hydroxyapatite	212:225	hydroxyapatite (HAP)	212:231	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	1	55	contain	containing	201:210	arg2	gelatin					248:254	gelatin	248:254	gelatin	248:254	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	1	55	contain	containing	201:210	arg1	scaffold					192:199	porous composite scaffold	175:199	porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method	175:365	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	7	56	theme	potential	1034:1042	arg1	biocompatibility					1044:1059	potential biocompatibility	1034:1059	potential biocompatibility	1034:1059	The cell migration and potential biocompatibility of nHAP-chitosan-gelatin scaffold was assessed via wound scratch assay and were compared to povedeen as control.					
31053693	0	57	theme	scaffold	65:72	arg1	evaluation					9:18	In vitro evaluation	0:18	In vitro evaluation of biodegradable nHAP-Chitosan-Gelatin-based scaffold for tissue engineering application.	0:108	In vitro evaluation of biodegradable nHAP-Chitosan-Gelatin-based scaffold for tissue engineering application.					
31053693	6	58	theme	Chitosan	948:955	arg1	gelatin					957:963	nano Chitosan gelatin	943:963	nano Chitosan gelatin	943:963	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	58	theme	Chitosan	948:955	arg1	Chitosan					933:940	the prepared nanocomposites [nano Chitosan	899:940	the prepared nanocomposites [nano Chitosan	899:940	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	7	59	theme	scaffold	1086:1093	arg1	migration					1020:1028	cell migration	1015:1028	cell migration	1015:1028	The cell migration and potential biocompatibility of nHAP-chitosan-gelatin scaffold was assessed via wound scratch assay and were compared to povedeen as control.					
31053693	7	59	theme	scaffold	1086:1093	arg1	biocompatibility					1044:1059	potential biocompatibility	1034:1059	potential biocompatibility	1034:1059	The cell migration and potential biocompatibility of nHAP-chitosan-gelatin scaffold was assessed via wound scratch assay and were compared to povedeen as control.					
31053693	6	60	theme	biodegradability	834:849	arg1	test					851:854	The biodegradability test	830:854	The biodegradability test	830:854	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	7	61	theme	cell	1015:1018	arg1	migration					1020:1028	cell migration	1015:1028	cell migration	1015:1028	The cell migration and potential biocompatibility of nHAP-chitosan-gelatin scaffold was assessed via wound scratch assay and were compared to povedeen as control.					
31053693	3	62	theme	functional	501:510	arg1	bonds					518:522	functional group bonds	501:522	functional group bonds of HAP powder, Chitosan and scaffold	501:559	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	6	63	theme	nano	943:946	arg1	gelatin					957:963	nano Chitosan gelatin	943:963	nano Chitosan gelatin	943:963	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	6	63	theme	nano	943:946	arg1	Chitosan					933:940	the prepared nanocomposites [nano Chitosan	899:940	the prepared nanocomposites [nano Chitosan	899:940	The biodegradability test was studied using Tris-Buffer solution for the prepared nanocomposites [nano Chitosan, nano Chitosan gelatin, Nano based Hydroxyapatite Chitosan gelatin].					
31053693	5	64	theme	simulated	787:795	arg1	solution					808:815	simulated body fluid solution	787:815	simulated body fluid solution	787:815	The Bioactivity of the Nano composite scaffolds was studied using simulated body fluid solution at 37 ± 1°C.					
31053693	5	65	theme	composite	749:757	arg1	scaffolds					759:767	the Nano composite scaffolds	740:767	the Nano composite scaffolds	740:767	The Bioactivity of the Nano composite scaffolds was studied using simulated body fluid solution at 37 ± 1°C.					
31053693	9	66	theme	potential	1439:1447	arg1	scaffolds					1425:1433	the developed nanocomposite scaffolds	1397:1433	the developed nanocomposite scaffolds	1397:1433	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	9	66	theme	potential	1439:1447	arg1	candidates					1449:1458	potential candidates	1439:1458	potential candidates for regenerating damaged cell tissue in wound healing process	1439:1520	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	1	67	with	hydroxyapatite	212:225	arg1	size					277:280	an average pore size	261:280	an average pore size of 250-1010 nm	261:295	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	9	68	theme	cell	1485:1488	arg1	tissue					1490:1495	damaged cell tissue	1477:1495	damaged cell tissue	1477:1495	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	8	69	theme	wound	1224:1228	arg1	assay					1238:1242	wound scratch assay	1224:1242	wound scratch assay	1224:1242	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	3	70	theme	HAP	527:529	arg1	powder					531:536	HAP powder	527:536	HAP powder	527:536	The presence of functional group bonds of HAP powder, Chitosan and scaffold was studied using Fourier Transform Infrared Spectroscopy (FTIR).					
31053693	8	71	theme	porous	1271:1276	arg1	scaffold					1292:1299	the fabricated porous nanocomposite scaffold	1256:1299	the fabricated porous nanocomposite scaffold	1256:1299	Cytocompatibility evaluation for Vero Cells using wound scratch assay showed that the fabricated porous nanocomposite scaffold possess higher cell proliferation and growth than that of povedeen.					
31053693	0	72	theme	engineering	85:95	arg1	application					97:107	tissue engineering application	78:107	tissue engineering application	78:107	In vitro evaluation of biodegradable nHAP-Chitosan-Gelatin-based scaffold for tissue engineering application.					
31053693	2	73	theme	X-Ray	388:392	arg1	XRD					407:409	XRD	407:409	XRD	407:409	From the results of X-Ray Diffraction (XRD) study, the peaks correspond to crystallographic structure of HAP powder.					
31053693	2	73	theme	X-Ray	388:392	arg1	Diffraction					394:404	X-Ray Diffraction	388:404	X-Ray Diffraction (XRD) study	388:416	From the results of X-Ray Diffraction (XRD) study, the peaks correspond to crystallographic structure of HAP powder.					
31053693	9	74	theme	healing	1506:1512	arg1	process					1514:1520	wound healing process	1500:1520	wound healing process	1500:1520	Thus, the study showed that the developed nanocomposite scaffolds are potential candidates for regenerating damaged cell tissue in wound healing process.					
31053693	0	75	theme	tissue	78:83	arg1	application					97:107	tissue engineering application	78:107	tissue engineering application	78:107	In vitro evaluation of biodegradable nHAP-Chitosan-Gelatin-based scaffold for tissue engineering application.					
31053693	1	76	theme	Electrospinning	344:358	arg1	method					360:365	Electrospinning method	344:365	Electrospinning method	344:365	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31053693	0	77	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro evaluation of biodegradable nHAP-Chitosan-Gelatin-based scaffold for tissue engineering application.					
31053693	1	78	theme	porous	175:180	arg1	scaffold					192:199	porous composite scaffold	175:199	porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method	175:365	The present study focuses on fabrication and characterisation of porous composite scaffold containing hydroxyapatite (HAP), chitosan, and gelatin with an average pore size of 250-1010 nm for improving wound repair and regeneration by Electrospinning method.					
31134431	2	0	theme	Bifidobacterium	350:364	arg1	ATCC					379:382	Bifidobacterium adolescentis ATCC 15703	350:388	Bifidobacterium adolescentis ATCC 15703	350:388	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	1	1	theme	composition	249:259	arg1	effect					215:220	the synbiotic effect	201:220	the synbiotic effect of prebiotic and probiotic composition against a specific pathogen	201:287	The present study is dedicated to the development of novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen.					
31134431	9	2	theme	different	1642:1650	arg1	prebiotics					1663:1672	different commercial prebiotics	1642:1672	different commercial prebiotics coupled with the Bifidobacterium adolescentis strain	1642:1725	This criterion has been examined for different commercial prebiotics coupled with the Bifidobacterium adolescentis strain.					
31134431	10	3	theme	synergistic	1732:1742	arg1	combination					1744:1754	The synergistic combination	1728:1754	The synergistic combination of inulin GR with bifidobacteria	1728:1787	The synergistic combination of inulin GR with bifidobacteria had the best effect against Bacillus cereus ATCC 9634.					
31134431	6	4	theme	co-culture	1161:1170	arg1	experiments					1172:1182	co-culture experiments	1161:1182	co-culture experiments	1161:1182	The yields of the inhibitors mentioned above were obtained from co-culture experiments.					
31134431	10	5	theme	inulin	1759:1764	arg1	GR					1766:1767	inulin GR	1759:1767	inulin GR	1759:1767	The synergistic combination of inulin GR with bifidobacteria had the best effect against Bacillus cereus ATCC 9634.					
31134431	4	6	theme	growth	683:688	arg1	rate					690:693	the specific growth rate	670:693	the specific growth rate of non-probiotics and short-chain fatty acid yields	670:745	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	10	7	theme	best	1797:1800	arg1	effect					1802:1807	the best effect	1793:1807	the best effect	1793:1807	The synergistic combination of inulin GR with bifidobacteria had the best effect against Bacillus cereus ATCC 9634.					
31134431	1	8	theme	criteria	178:185	arg1	development					157:167	the development	153:167	the development of novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen	153:287	The present study is dedicated to the development of novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen.					
31134431	9	9	theme	Bifidobacterium	1691:1705	arg1	strain					1720:1725	the Bifidobacterium adolescentis strain	1687:1725	the Bifidobacterium adolescentis strain	1687:1725	This criterion has been examined for different commercial prebiotics coupled with the Bifidobacterium adolescentis strain.					
31134431	5	10	theme	growth	1051:1056	arg1	rate					1058:1061	the specific growth rate	1038:1061	the specific growth rate	1038:1061	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	3	11	theme	specific	558:565	arg1	rate					574:577	the specific growth rate	554:577	the specific growth rate of non-probiotics	554:595	The model is based on the hypothesis that probiotics can reduce the specific growth rate of non-probiotics by producing short-chain fatty acids.					
31134431	5	12	theme	different	917:925	arg1	equations					927:935	Two different equations	913:935	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki)	913:1015	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	10	13	theme	cereus	1826:1831	arg1	ATCC					1833:1836	Bacillus cereus ATCC 9634	1817:1841	Bacillus cereus ATCC 9634	1817:1841	The synergistic combination of inulin GR with bifidobacteria had the best effect against Bacillus cereus ATCC 9634.					
31134431	1	14	theme	synbiotic	205:213	arg1	effect					215:220	the synbiotic effect	201:220	the synbiotic effect of prebiotic and probiotic composition against a specific pathogen	201:287	The present study is dedicated to the development of novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen.					
31134431	4	15	theme	fatty	729:733	arg1	acid					735:738	short-chain fatty acid	717:738	short-chain fatty acid	717:738	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	3	16	theme	non-probiotics	582:595	arg1	rate					574:577	the specific growth rate	554:577	the specific growth rate of non-probiotics	554:595	The model is based on the hypothesis that probiotics can reduce the specific growth rate of non-probiotics by producing short-chain fatty acids.					
31134431	4	17	theme	pure	888:891	arg1	culture					893:899	a pure culture	886:899	a pure culture of bacilli	886:910	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	7	18	theme	competition	1325:1335	arg1	experiments					1337:1347	the competition experiments	1321:1347	the competition experiments	1321:1347	The experimental values and the values predicted by the model of Bacillus count did not differ significantly (R2 not less than 0.83) in the competition experiments.					
31134431	0	19	theme	growth	50:55	arg1	inhibition					57:66	growth inhibition	50:66	growth inhibition by bifidobacteria	50:84	A quantitative model of Bacillus cereus ATCC 9634 growth inhibition by bifidobacteria for synbiotic effect evaluation.					
31134431	3	20	theme	fatty	622:626	arg1	acids					628:632	short-chain fatty acids	610:632	short-chain fatty acids	610:632	The model is based on the hypothesis that probiotics can reduce the specific growth rate of non-probiotics by producing short-chain fatty acids.					
31134431	8	21	theme	non-probiotic	1466:1478	arg1	reduction					1501:1509	non-probiotic specific growth rate reduction	1466:1509	non-probiotic specific growth rate reduction	1466:1509	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	10	22	contain	had	1789:1791	arg2	effect					1802:1807	the best effect	1793:1807	the best effect	1793:1807	The synergistic combination of inulin GR with bifidobacteria had the best effect against Bacillus cereus ATCC 9634.					
31134431	10	22	contain	had	1789:1791	arg1	combination					1744:1754	The synergistic combination	1728:1754	The synergistic combination of inulin GR with bifidobacteria	1728:1787	The synergistic combination of inulin GR with bifidobacteria had the best effect against Bacillus cereus ATCC 9634.					
31134431	8	23	theme	growth	1489:1494	arg1	reduction					1501:1509	non-probiotic specific growth rate reduction	1466:1509	non-probiotic specific growth rate reduction	1466:1509	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	4	24	dep	non-probiotics	698:711	arg1	yields					740:745	yields	740:745	yields	740:745	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	8	25	theme	reduction	1501:1509	arg1	coefficient					1451:1461	the coefficient	1447:1461	the coefficient of non-probiotic specific growth rate reduction	1447:1509	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	8	25	theme	reduction	1501:1509	arg1	result					1516:1521	a result	1514:1521	a result of probiotic growth and inhibitor formation	1514:1565	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	2	26	theme	ATCC	410:413	arg1	model					341:345	the quantitative model	324:345	the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition	324:460	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	0	27	theme	effect	100:105	arg1	evaluation					107:116	synbiotic effect evaluation	90:116	synbiotic effect evaluation	90:116	A quantitative model of Bacillus cereus ATCC 9634 growth inhibition by bifidobacteria for synbiotic effect evaluation.					
31134431	7	28	dep	differ	1273:1278	arg1	R2					1295:1296	R2	1295:1296	R2 not less than 0.83	1295:1315	The experimental values and the values predicted by the model of Bacillus count did not differ significantly (R2 not less than 0.83) in the competition experiments.					
31134431	2	29	theme	Bacillus	394:401	arg1	ATCC					410:413	Bacillus cereus ATCC 9634	394:418	Bacillus cereus ATCC 9634 (as a model food contaminant)	394:448	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	1	30	theme	specific	271:278	arg1	pathogen					280:287	a specific pathogen	269:287	a specific pathogen	269:287	The present study is dedicated to the development of novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen.					
31134431	2	31	theme	food	432:435	arg1	contaminant					437:447	a model food contaminant	424:447	a model food contaminant	424:447	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	5	32	theme	inhibitor	958:966	arg1	MIC					983:985	MIC	983:985	MIC	983:985	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	5	32	theme	inhibitor	958:966	arg1	concentration					968:980	the minimum inhibitor concentration	946:980	the minimum inhibitor concentration (MIC)	946:986	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	8	33	theme	growth	1536:1541	arg1	coefficient					1451:1461	the coefficient	1447:1461	the coefficient of non-probiotic specific growth rate reduction	1447:1509	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	8	33	theme	growth	1536:1541	arg1	result					1516:1521	a result	1514:1521	a result of probiotic growth and inhibitor formation	1514:1565	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	0	34	theme	Bacillus	24:31	arg1	ATCC					40:43	Bacillus cereus ATCC	24:43	Bacillus cereus ATCC	24:43	A quantitative model of Bacillus cereus ATCC 9634 growth inhibition by bifidobacteria for synbiotic effect evaluation.					
31134431	8	35	theme	inhibitor	1547:1555	arg1	formation					1557:1565	inhibitor formation	1547:1565	inhibitor formation	1547:1565	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	8	36	attach	derived	1411:1417	arg1	model					1428:1432	the model	1424:1432	the model	1424:1432	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	8	36	attach	derived	1411:1417	arg2	criterion					1373:1381	the general criterion	1361:1381	the general criterion of the synbiotic effect	1361:1405	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	0	37	dep	Bacillus	24:31	arg1	cereus					33:38	cereus	33:38	cereus	33:38	A quantitative model of Bacillus cereus ATCC 9634 growth inhibition by bifidobacteria for synbiotic effect evaluation.					
31134431	8	38	theme	effect	1400:1405	arg1	criterion					1373:1381	the general criterion	1361:1381	the general criterion of the synbiotic effect	1361:1405	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	2	39	theme	quantitative	328:339	arg1	model					341:345	the quantitative model	324:345	the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition	324:460	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	5	40	theme	inhibition	992:1001	arg1	constant					1003:1010	inhibition constant	992:1010	inhibition constant (Ki)	992:1015	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	5	40	theme	inhibition	992:1001	arg1	Ki					1013:1014	Ki	1013:1014	Ki	1013:1014	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	8	41	theme	final	1574:1578	arg1	fermentation					1591:1602	the final co-culture fermentation	1570:1602	the final co-culture fermentation	1570:1602	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	5	42	used	used	1022:1025	arg2	equations					927:935	Two different equations	913:935	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki)	913:1015	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	1	43	theme	prebiotic	225:233	arg1	composition					249:259	prebiotic and probiotic composition	225:259	prebiotic and probiotic composition	225:259	The present study is dedicated to the development of novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen.					
31134431	7	44	theme	Bacillus	1250:1257	arg1	count					1259:1263	Bacillus count	1250:1263	Bacillus count	1250:1263	The experimental values and the values predicted by the model of Bacillus count did not differ significantly (R2 not less than 0.83) in the competition experiments.					
31134431	2	45	theme	adolescentis	366:377	arg1	ATCC					379:382	Bifidobacterium adolescentis ATCC 15703	350:388	Bifidobacterium adolescentis ATCC 15703	350:388	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	1	46	theme	probiotic	239:247	arg1	composition					249:259	prebiotic and probiotic composition	225:259	prebiotic and probiotic composition	225:259	The present study is dedicated to the development of novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen.					
31134431	9	47	theme	commercial	1652:1661	arg1	prebiotics					1663:1672	different commercial prebiotics	1642:1672	different commercial prebiotics coupled with the Bifidobacterium adolescentis strain	1642:1725	This criterion has been examined for different commercial prebiotics coupled with the Bifidobacterium adolescentis strain.					
31134431	10	48	theme	GR	1766:1767	arg1	combination					1744:1754	The synergistic combination	1728:1754	The synergistic combination of inulin GR with bifidobacteria	1728:1787	The synergistic combination of inulin GR with bifidobacteria had the best effect against Bacillus cereus ATCC 9634.					
31134431	0	49	theme	ATCC	40:43	arg1	model					15:19	A quantitative model	0:19	A quantitative model of Bacillus cereus ATCC	0:43	A quantitative model of Bacillus cereus ATCC 9634 growth inhibition by bifidobacteria for synbiotic effect evaluation.					
31134431	4	50	theme	acetic	855:860	arg1	acids					876:880	lactic, acetic and propionic acids	847:880	lactic, acetic and propionic acids	847:880	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	4	50	theme	acetic	855:860	arg1	bifidobacteria					831:844	bifidobacteria	831:844	bifidobacteria (lactic, acetic and propionic acids)	831:881	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	3	51	theme	growth	567:572	arg1	rate					574:577	the specific growth rate	554:577	the specific growth rate of non-probiotics	554:595	The model is based on the hypothesis that probiotics can reduce the specific growth rate of non-probiotics by producing short-chain fatty acids.					
31134431	1	52	theme	novel	172:176	arg1	criteria					178:185	novel criteria	172:185	novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen	172:287	The present study is dedicated to the development of novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen.					
31134431	4	53	theme	propionic	866:874	arg1	acids					876:880	lactic, acetic and propionic acids	847:880	lactic, acetic and propionic acids	847:880	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	4	53	theme	propionic	866:874	arg1	bifidobacteria					831:844	bifidobacteria	831:844	bifidobacteria (lactic, acetic and propionic acids)	831:881	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	4	54	theme	specific	674:681	arg1	rate					690:693	the specific growth rate	670:693	the specific growth rate of non-probiotics and short-chain fatty acid yields	670:745	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	9	55	theme	adolescentis	1707:1718	arg1	strain					1720:1725	the Bifidobacterium adolescentis strain	1687:1725	the Bifidobacterium adolescentis strain	1687:1725	This criterion has been examined for different commercial prebiotics coupled with the Bifidobacterium adolescentis strain.					
31134431	10	56	theme	Bacillus	1817:1824	arg1	ATCC					1833:1836	Bacillus cereus ATCC 9634	1817:1841	Bacillus cereus ATCC 9634	1817:1841	The synergistic combination of inulin GR with bifidobacteria had the best effect against Bacillus cereus ATCC 9634.					
31134431	4	57	theme	short-chain	717:727	arg1	acid					735:738	short-chain fatty acid	717:738	short-chain fatty acid	717:738	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	2	58	dep	Bacillus	394:401	arg1	cereus					403:408	cereus	403:408	cereus	403:408	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	1	59	theme	present	123:129	arg1	study					131:135	The present study	119:135	The present study	119:135	The present study is dedicated to the development of novel criteria for assessing the synbiotic effect of prebiotic and probiotic composition against a specific pathogen.					
31134431	4	60	theme	bacilli	904:910	arg1	culture					893:899	a pure culture	886:899	a pure culture of bacilli	886:910	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	4	61	theme	acid	735:738	arg1	rate					690:693	the specific growth rate	670:693	the specific growth rate of non-probiotics and short-chain fatty acid yields	670:745	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	5	62	theme	specific	1042:1049	arg1	rate					1058:1061	the specific growth rate	1038:1061	the specific growth rate	1038:1061	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	3	63	theme	short-chain	610:620	arg1	acids					628:632	short-chain fatty acids	610:632	short-chain fatty acids	610:632	The model is based on the hypothesis that probiotics can reduce the specific growth rate of non-probiotics by producing short-chain fatty acids.					
31134431	8	64	theme	specific	1480:1487	arg1	reduction					1501:1509	non-probiotic specific growth rate reduction	1466:1509	non-probiotic specific growth rate reduction	1466:1509	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	8	65	theme	rate	1496:1499	arg1	reduction					1501:1509	non-probiotic specific growth rate reduction	1466:1509	non-probiotic specific growth rate reduction	1466:1509	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	4	66	theme	non-probiotics	698:711	arg1	rate					690:693	the specific growth rate	670:693	the specific growth rate of non-probiotics and short-chain fatty acid yields	670:745	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	0	67	theme	synbiotic	90:98	arg1	evaluation					107:116	synbiotic effect evaluation	90:116	synbiotic effect evaluation	90:116	A quantitative model of Bacillus cereus ATCC 9634 growth inhibition by bifidobacteria for synbiotic effect evaluation.					
31134431	5	68	theme	inhibitors	1085:1094	arg1	rate					1058:1061	the specific growth rate	1038:1061	the specific growth rate	1038:1061	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	5	68	theme	inhibitors	1085:1094	arg1	concentrations					1067:1080	concentrations	1067:1080	concentrations of inhibitors	1067:1094	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	2	69	dep	ATCC	379:382	arg1	competition					450:460	competition	450:460	competition	450:460	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	0	70	theme	quantitative	2:13	arg1	model					15:19	A quantitative model	0:19	A quantitative model of Bacillus cereus ATCC	0:43	A quantitative model of Bacillus cereus ATCC 9634 growth inhibition by bifidobacteria for synbiotic effect evaluation.					
31134431	7	71	theme	less	1302:1305	arg1	R2					1295:1296	R2	1295:1296	R2 not less than 0.83	1295:1315	The experimental values and the values predicted by the model of Bacillus count did not differ significantly (R2 not less than 0.83) in the competition experiments.					
31134431	8	72	theme	probiotic	1526:1534	arg1	growth					1536:1541	probiotic growth	1526:1541	probiotic growth	1526:1541	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	5	73	theme	minimum	950:956	arg1	MIC					983:985	MIC	983:985	MIC	983:985	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	5	73	theme	minimum	950:956	arg1	concentration					968:980	the minimum inhibitor concentration	946:980	the minimum inhibitor concentration (MIC)	946:986	Two different equations based on the minimum inhibitor concentration (MIC) and inhibition constant (Ki) were used to connect the specific growth rate and concentrations of inhibitors.					
31134431	2	74	theme	model	426:430	arg1	contaminant					437:447	a model food contaminant	424:447	a model food contaminant	424:447	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	4	75	theme	inhibition	752:761	arg1	constants					763:771	the inhibition constants	748:771	the inhibition constants	748:771	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	2	76	theme	co-culture	465:474	arg1	fermentation					476:487	co-culture fermentation	465:487	co-culture fermentation	465:487	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	8	77	theme	formation	1557:1565	arg1	coefficient					1451:1461	the coefficient	1447:1461	the coefficient of non-probiotic specific growth rate reduction	1447:1509	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	8	77	theme	formation	1557:1565	arg1	result					1516:1521	a result	1514:1521	a result of probiotic growth and inhibitor formation	1514:1565	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	8	78	theme	general	1365:1371	arg1	criterion					1373:1381	the general criterion	1361:1381	the general criterion of the synbiotic effect	1361:1405	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	7	79	theme	experimental	1189:1200	arg1	values					1202:1207	The experimental values	1185:1207	The experimental values	1185:1207	The experimental values and the values predicted by the model of Bacillus count did not differ significantly (R2 not less than 0.83) in the competition experiments.					
31134431	6	80	theme	inhibitors	1115:1124	arg1	yields					1101:1106	The yields	1097:1106	The yields of the inhibitors mentioned above	1097:1140	The yields of the inhibitors mentioned above were obtained from co-culture experiments.					
31134431	4	81	theme	lactic	847:852	arg1	acids					876:880	lactic, acetic and propionic acids	847:880	lactic, acetic and propionic acids	847:880	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	4	81	theme	lactic	847:852	arg1	bifidobacteria					831:844	bifidobacteria	831:844	bifidobacteria (lactic, acetic and propionic acids)	831:881	To define the relationship between the specific growth rate of non-probiotics and short-chain fatty acid yields, the inhibition constants were determined separately for each inhibitor produced by bifidobacteria (lactic, acetic and propionic acids) in a pure culture of bacilli.					
31134431	7	82	theme	count	1259:1263	arg1	model					1241:1245	the model	1237:1245	the model of Bacillus count	1237:1263	The experimental values and the values predicted by the model of Bacillus count did not differ significantly (R2 not less than 0.83) in the competition experiments.					
31134431	8	83	theme	co-culture	1580:1589	arg1	fermentation					1591:1602	the final co-culture fermentation	1570:1602	the final co-culture fermentation	1570:1602	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	8	84	theme	synbiotic	1390:1398	arg1	effect					1400:1405	the synbiotic effect	1386:1405	the synbiotic effect	1386:1405	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	10	85	with	combination	1744:1754	arg1	bifidobacteria					1774:1787	bifidobacteria	1774:1787	bifidobacteria	1774:1787	The synergistic combination of inulin GR with bifidobacteria had the best effect against Bacillus cereus ATCC 9634.					
31134431	2	86	theme	ATCC	379:382	arg1	model					341:345	the quantitative model	324:345	the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition	324:460	These criteria were obtained from the quantitative model of Bifidobacterium adolescentis ATCC 15703 and Bacillus cereus ATCC 9634 (as a model food contaminant) competition in co-culture fermentation.					
31134431	8	87	attach	presents	1438:1445	arg1	fermentation					1591:1602	the final co-culture fermentation	1570:1602	the final co-culture fermentation	1570:1602	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31134431	8	87	attach	presents	1438:1445	arg2	criterion					1373:1381	the general criterion	1361:1381	the general criterion of the synbiotic effect	1361:1405	Therefore, the general criterion of the synbiotic effect was derived from the model and presents the coefficient of non-probiotic specific growth rate reduction as a result of probiotic growth and inhibitor formation in the final co-culture fermentation.					
31635735	5	0	theme	significant	782:792	arg1	type					754:757	Sugar type	748:757	Sugar type	748:757	Sugar type was found to be a very significant factor affecting gel characteristics and retrogradation.					
31635735	5	0	theme	significant	782:792	arg1	factor					794:799	a very significant factor	775:799	a very significant factor affecting gel characteristics and retrogradation	775:848	Sugar type was found to be a very significant factor affecting gel characteristics and retrogradation.					
31635735	0	1	theme	gel	49:51	arg1	matrix					53:58	a starch based composite gel matrix	24:58	a starch based composite gel matrix	24:58	Role of 'D-allulose' in a starch based composite gel matrix.					
31635735	8	2	theme	NMR	1164:1166	arg1	experiments					1168:1178	NMR experiments	1164:1178	NMR experiments	1164:1178	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	6	3	theme	gel	1013:1015	arg1	network					1017:1023	better gel network	1006:1023	better gel network	1006:1023	Results showed that D-allulose containing formulations were less prone to retrogradation and showed smaller changes upon storage by supporting presence of better gel network.					
31635735	8	4	theme	sugar	1313:1317	arg1	type					1305:1308	different type	1295:1308	different type of sugar	1295:1317	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	0	5	from	Role	0:3	arg1	matrix					53:58	a starch based composite gel matrix	24:58	a starch based composite gel matrix	24:58	Role of 'D-allulose' in a starch based composite gel matrix.					
31635735	2	6	theme	non-caloric	255:265	arg1	sugar					272:276	a non-caloric rare sugar	253:276	a non-caloric rare sugar	253:276	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	2	6	theme	non-caloric	255:265	arg1	D-allulose					279:288	D-allulose	279:288	D-allulose (formerly called D-psicose)	279:316	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	2	7	theme	rare	267:270	arg1	sugar					272:276	a non-caloric rare sugar	253:276	a non-caloric rare sugar	253:276	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	2	7	theme	rare	267:270	arg1	D-allulose					279:288	D-allulose	279:288	D-allulose (formerly called D-psicose)	279:316	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	4	8	theme	Nuclear	648:654	arg1	TD-NMR					676:681	TD-NMR	676:681	TD-NMR	676:681	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	4	8	theme	Nuclear	648:654	arg1	Resonance					665:673	Time Domain Nuclear Magnetic Resonance	636:673	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique	636:692	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	1	9	contain	have	129:132	arg1	agents					87:92	gelling agents	79:92	gelling agents used in confectionery formulations	79:127	Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage.					
31635735	1	9	contain	have	129:132	arg1	Type					61:64	Type	61:64	Type of sugar	61:73	Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage.					
31635735	1	9	contain	have	129:132	arg2	importance					140:149	vital importance	134:149	vital importance	134:149	Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage.					
31635735	7	10	theme	X-ray	1039:1043	arg1	results					1045:1051	X-ray results	1039:1051	X-ray results	1039:1051	According to X-ray results, sucrose containing formulations were more susceptible to crystallization.					
31635735	6	11	theme	network	1017:1023	arg1	presence					994:1001	presence	994:1001	presence of better gel network	994:1023	Results showed that D-allulose containing formulations were less prone to retrogradation and showed smaller changes upon storage by supporting presence of better gel network.					
31635735	5	12	theme	Sugar	748:752	arg1	factor					794:799	a very significant factor	775:799	a very significant factor affecting gel characteristics and retrogradation	775:848	Sugar type was found to be a very significant factor affecting gel characteristics and retrogradation.					
31635735	5	12	theme	Sugar	748:752	arg1	type					754:757	Sugar type	748:757	Sugar type	748:757	Sugar type was found to be a very significant factor affecting gel characteristics and retrogradation.					
31635735	0	13	theme	starch	26:31	arg1	matrix					53:58	a starch based composite gel matrix	24:58	a starch based composite gel matrix	24:58	Role of 'D-allulose' in a starch based composite gel matrix.					
31635735	8	14	theme	distinct	1202:1209	arg1	peaks					1211:1215	distinct peaks	1202:1215	distinct peaks	1202:1215	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	8	15	theme	different	1295:1303	arg1	type					1305:1308	different type	1295:1308	different type of sugar	1295:1317	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	1	16	theme	vital	134:138	arg1	importance					140:149	vital importance	134:149	vital importance	134:149	Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage.					
31635735	7	17	theme	sucrose	1054:1060	arg1	formulations					1073:1084	sucrose containing formulations	1054:1084	sucrose containing formulations	1054:1084	According to X-ray results, sucrose containing formulations were more susceptible to crystallization.					
31635735	2	18	theme	soy	406:408	arg1	isolate					418:424	soy protein isolate	406:424	soy protein isolate (SPI)	406:430	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	2	18	theme	soy	406:408	arg1	SPI					427:429	SPI	427:429	SPI	427:429	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	0	19	theme	composite	39:47	arg1	matrix					53:58	a starch based composite gel matrix	24:58	a starch based composite gel matrix	24:58	Role of 'D-allulose' in a starch based composite gel matrix.					
31635735	3	20	theme	gel	524:526	arg1	systems					528:534	the formulized gel systems	509:534	the formulized gel systems	509:534	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	2	21	theme	confectionery	338:350	arg1	gels					352:355	the starch based confectionery gels	321:355	the starch based confectionery gels	321:355	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	3	22	theme	formulized	513:522	arg1	systems					528:534	the formulized gel systems	509:534	the formulized gel systems	509:534	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	3	23	theme	systems	528:534	arg1	characterization					489:504	characterization	489:504	characterization of the formulized gel systems	489:534	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	2	24	theme	based	332:336	arg1	gels					352:355	the starch based confectionery gels	321:355	the starch based confectionery gels	321:355	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	0	25	theme	D-allulose	9:18	arg1	Role					0:3	Role	0:3	Role of 'D-allulose' in a starch based composite gel matrix.	0:59	Role of 'D-allulose' in a starch based composite gel matrix.					
31635735	6	26	theme	containing	882:891	arg1	formulations					893:904	D-allulose containing formulations	871:904	D-allulose containing formulations	871:904	Results showed that D-allulose containing formulations were less prone to retrogradation and showed smaller changes upon storage by supporting presence of better gel network.					
31635735	2	27	theme	sugar	272:276	arg1	effect					243:248	the effect	239:248	the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels	239:355	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	3	28	dep	used	560:563	arg1	SEM					566:568	SEM	566:568	SEM	566:568	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	4	29	theme	Domain	641:646	arg1	TD-NMR					676:681	TD-NMR	676:681	TD-NMR	676:681	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	4	29	theme	Domain	641:646	arg1	Resonance					665:673	Time Domain Nuclear Magnetic Resonance	636:673	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique	636:692	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	6	30	theme	D-allulose	871:880	arg1	formulations					893:904	D-allulose containing formulations	871:904	D-allulose containing formulations	871:904	Results showed that D-allulose containing formulations were less prone to retrogradation and showed smaller changes upon storage by supporting presence of better gel network.					
31635735	1	31	theme	sugar	69:73	arg1	agents					87:92	gelling agents	79:92	gelling agents used in confectionery formulations	79:127	Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage.					
31635735	1	31	theme	sugar	69:73	arg1	Type					61:64	Type	61:64	Type of sugar	61:73	Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage.					
31635735	8	32	theme	relaxation	1256:1265	arg1	times					1267:1271	relaxation times	1256:1271	relaxation times of peaks	1256:1280	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	2	33	theme	called	300:305	arg1	D-psicose					307:315	formerly called D-psicose	291:315	formerly called D-psicose	291:315	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	2	33	theme	called	300:305	arg1	D-allulose					279:288	D-allulose	279:288	D-allulose (formerly called D-psicose)	279:316	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	3	34	dep	SEM	566:568	arg1	DSC					571:573	DSC	571:573	DSC	571:573	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	3	34	dep	SEM	566:568	arg1	XRD					576:578	XRD	576:578	XRD	576:578	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	3	34	dep	SEM	566:568	arg1	activity					605:612	water activity	599:612	water activity	599:612	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	3	34	dep	SEM	566:568	arg1	hardness					615:622	hardness	615:622	hardness	615:622	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	3	34	dep	SEM	566:568	arg1	content					590:596	moisture content	581:596	moisture content	581:596	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	3	34	dep	SEM	566:568	arg1	color					628:632	color	628:632	color	628:632	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	1	35	theme	physicochemical	181:195	arg1	properties					197:206	physicochemical properties	181:206	physicochemical properties	181:206	Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage.					
31635735	6	36	theme	better	1006:1011	arg1	network					1017:1023	better gel network	1006:1023	better gel network	1006:1023	Results showed that D-allulose containing formulations were less prone to retrogradation and showed smaller changes upon storage by supporting presence of better gel network.					
31635735	5	37	theme	gel	811:813	arg1	characteristics					815:829	gel characteristics	811:829	gel characteristics	811:829	Sugar type was found to be a very significant factor affecting gel characteristics and retrogradation.					
31635735	4	38	theme	Resonance	665:673	arg1	technique					684:692	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique	636:692	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique	636:692	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	2	39	theme	isolate	418:424	arg1	absence					395:401	absence	395:401	absence	395:401	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	2	39	theme	isolate	418:424	arg1	presence					382:389	presence	382:389	presence	382:389	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	0	40	theme	based	33:37	arg1	matrix					53:58	a starch based composite gel matrix	24:58	a starch based composite gel matrix	24:58	Role of 'D-allulose' in a starch based composite gel matrix.					
31635735	2	41	dep	presence	382:389	arg1	the					378:380	the	378:380	the	378:380	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	2	42	theme	protein	410:416	arg1	isolate					418:424	soy protein isolate	406:424	soy protein isolate (SPI)	406:430	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	2	42	theme	protein	410:416	arg1	SPI					427:429	SPI	427:429	SPI	427:429	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	3	43	theme	common	537:542	arg1	techniques					544:553	common techniques	537:553	common techniques	537:553	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	4	44	from	dynamics	723:730	arg1	systems					739:745	the systems	735:745	the systems	735:745	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	1	45	theme	gelling	79:85	arg1	agents					87:92	gelling agents	79:92	gelling agents used in confectionery formulations	79:127	Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage.					
31635735	8	46	theme	T2	1128:1129	arg1	spectra					1142:1148	T2 relaxation spectra	1128:1148	T2 relaxation spectra obtained from NMR experiments	1128:1178	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	3	47	theme	water	599:603	arg1	activity					605:612	water activity	599:612	water activity	599:612	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	7	48	theme	containing	1062:1071	arg1	formulations					1073:1084	sucrose containing formulations	1054:1084	sucrose containing formulations	1054:1084	According to X-ray results, sucrose containing formulations were more susceptible to crystallization.					
31635735	4	49	theme	Time	636:639	arg1	TD-NMR					676:681	TD-NMR	676:681	TD-NMR	676:681	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	4	49	theme	Time	636:639	arg1	Resonance					665:673	Time Domain Nuclear Magnetic Resonance	636:673	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique	636:692	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	2	50	from	effect	243:248	arg1	gels					352:355	the starch based confectionery gels	321:355	the starch based confectionery gels	321:355	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	6	51	theme	smaller	951:957	arg1	changes					959:965	smaller changes	951:965	smaller changes upon storage	951:978	Results showed that D-allulose containing formulations were less prone to retrogradation and showed smaller changes upon storage by supporting presence of better gel network.					
31635735	3	52	theme	moisture	581:588	arg1	content					590:596	moisture content	581:596	moisture content	581:596	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	4	53	theme	Magnetic	656:663	arg1	TD-NMR					676:681	TD-NMR	676:681	TD-NMR	676:681	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	4	53	theme	Magnetic	656:663	arg1	Resonance					665:673	Time Domain Nuclear Magnetic Resonance	636:673	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique	636:692	Time Domain Nuclear Magnetic Resonance (TD-NMR) technique was also employed to explain dynamics in the systems.					
31635735	3	54	used	used	560:563	arg2	techniques					544:553	common techniques	537:553	common techniques	537:553	For characterization of the formulized gel systems, common techniques were used (SEM, DSC, XRD, moisture content, water activity, hardness and color).					
31635735	8	55	theme	peaks	1276:1280	arg1	times					1267:1271	relaxation times	1256:1271	relaxation times of peaks	1256:1280	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	8	56	theme	SPI	1246:1248	arg1	addition					1234:1241	the addition	1230:1241	the addition of SPI	1230:1248	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	2	57	theme	experimental	448:459	arg1	techniques					461:470	different experimental techniques	438:470	different experimental techniques for 28 days	438:482	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	8	58	theme	peaks	1211:1215	arg1	number					1192:1197	number	1192:1197	number of distinct peaks	1192:1215	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	2	59	theme	starch	325:330	arg1	gels					352:355	the starch based confectionery gels	321:355	the starch based confectionery gels	321:355	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	2	60	theme	different	438:446	arg1	techniques					461:470	different experimental techniques	438:470	different experimental techniques for 28 days	438:482	In this study, the effect of a non-caloric rare sugar, D-allulose (formerly called D-psicose) on the starch based confectionery gels were investigated in the presence and absence of soy protein isolate (SPI) using different experimental techniques for 28 days.					
31635735	8	61	theme	relaxation	1131:1140	arg1	spectra					1142:1148	T2 relaxation spectra	1128:1148	T2 relaxation spectra obtained from NMR experiments	1128:1178	T2 relaxation spectra obtained from NMR experiments showed that number of distinct peaks reduced with the addition of SPI while relaxation times of peaks changed when different type of sugar.					
31635735	1	62	theme	confectionery	102:114	arg1	formulations					116:127	confectionery formulations	102:127	confectionery formulations	102:127	Type of sugar and gelling agents used in confectionery formulations have vital importance since they directly influence physicochemical properties during storage.					
31362024	0	0	theme	nanocomposite	92:104	arg1	membranes					106:114	the porous transparent BaSO4/cellulose nanocomposite membranes	53:114	the porous transparent BaSO4/cellulose nanocomposite membranes	53:114	X-ray shielding structural and properties design for the porous transparent BaSO4/cellulose nanocomposite membranes.					
31362024	6	1	theme	BS	953:954	arg1	load					945:948	the load	941:948	the load of BS	941:954	Brunauer-Emmett-Teller (BET) indicated that the load of BS contributed little to the specific surface area and pore size.					
31362024	10	2	theme	X-ray	1380:1384	arg1	material					1406:1413	X-ray radiation shielding material	1380:1413	X-ray radiation shielding material	1380:1413	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	10	2	theme	X-ray	1380:1384	arg1	BSCM					1359:1362	the BSCM	1355:1362	the BSCM	1355:1362	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	1	3	theme	medical	169:175	arg1	fields					199:204	medical, aviation and nuclear fields	169:204	medical, aviation and nuclear fields	169:204	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	6	4	theme	surface	991:997	arg1	area					999:1002	the specific surface area	978:1002	the specific surface area	978:1002	Brunauer-Emmett-Teller (BET) indicated that the load of BS contributed little to the specific surface area and pore size.					
31362024	0	5	theme	BaSO4/cellulose	76:90	arg1	membranes					106:114	the porous transparent BaSO4/cellulose nanocomposite membranes	53:114	the porous transparent BaSO4/cellulose nanocomposite membranes	53:114	X-ray shielding structural and properties design for the porous transparent BaSO4/cellulose nanocomposite membranes.					
31362024	7	6	theme	same	1091:1094	arg1	level					1096:1100	the same level	1087:1100	the same level	1087:1100	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	2	7	from	effects	343:349	arg1	properties					426:435	the physical and morphological properties	395:435	the physical and morphological properties of the cellulose membrane (CM)	395:466	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	2	8	theme	nano-BaSO4	367:376	arg1	addition					383:390	carboxylated nano-BaSO4 (BS) addition	354:390	carboxylated nano-BaSO4 (BS) addition	354:390	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	10	9	theme	filler	1427:1432	arg1	material					1434:1441	sandwich filler material	1418:1441	sandwich filler material in the implication of radiation shielding glass	1418:1489	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	10	9	theme	filler	1427:1432	arg1	BSCM					1359:1362	the BSCM	1355:1362	the BSCM	1355:1362	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	1	10	theme	BSCM	271:274	arg1	material					277:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material	207:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure	207:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	4	11	theme	cellulose	738:746	arg1	surface					748:754	the cellulose surface	734:754	the cellulose surface	734:754	Scanning electron microscopy (SEM) images displayed the aggregations of BS in the cellulose surface.					
31362024	10	12	from	material	1434:1441	arg1	implication					1450:1460	the implication	1446:1460	the implication of radiation shielding glass	1446:1489	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	4	13	theme	BS	728:729	arg1	aggregations					712:723	the aggregations	708:723	the aggregations of BS	708:729	Scanning electron microscopy (SEM) images displayed the aggregations of BS in the cellulose surface.					
31362024	8	14	theme	BS	1244:1245	arg1	addition					1232:1239	the addition	1228:1239	the addition of BS	1228:1245	The swelling ratios, weight loss ratios and mechanical property were decreased along with the addition of BS.					
31362024	9	15	theme	radiation	1252:1260	arg1	ability					1272:1278	The radiation shielding ability	1248:1278	The radiation shielding ability	1248:1278	The radiation shielding ability was enhanced.					
31362024	3	16	theme	capacity	531:538	arg1	Meanwhile					487:495	Meanwhile	487:495	Meanwhile	487:495	Meanwhile, the influence of X-ray shielding capacity was studied by different layers of composite membranes and the shielding mechanism of the X-ray was also discussed.					
31362024	3	16	theme	capacity	531:538	arg1	influence					502:510	the influence	498:510	the influence of X-ray shielding capacity	498:538	Meanwhile, the influence of X-ray shielding capacity was studied by different layers of composite membranes and the shielding mechanism of the X-ray was also discussed.					
31362024	1	17	theme	aviation	178:185	arg1	fields					199:204	medical, aviation and nuclear fields	169:204	medical, aviation and nuclear fields	169:204	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	8	18	theme	loss	1166:1169	arg1	ratios					1171:1176	weight loss ratios	1159:1176	weight loss ratios	1159:1176	The swelling ratios, weight loss ratios and mechanical property were decreased along with the addition of BS.					
31362024	9	19	theme	shielding	1262:1270	arg1	ability					1272:1278	The radiation shielding ability	1248:1278	The radiation shielding ability	1248:1278	The radiation shielding ability was enhanced.					
31362024	10	20	theme	sandwich	1418:1425	arg1	material					1434:1441	sandwich filler material	1418:1441	sandwich filler material in the implication of radiation shielding glass	1418:1489	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	10	20	theme	sandwich	1418:1425	arg1	BSCM					1359:1362	the BSCM	1355:1362	the BSCM	1355:1362	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	2	21	theme	membrane	454:461	arg1	properties					426:435	the physical and morphological properties	395:435	the physical and morphological properties of the cellulose membrane (CM)	395:466	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	4	22	theme	Scanning	656:663	arg1	SEM					686:688	SEM	686:688	SEM	686:688	Scanning electron microscopy (SEM) images displayed the aggregations of BS in the cellulose surface.					
31362024	4	22	theme	Scanning	656:663	arg1	microscopy					674:683	Scanning electron microscopy	656:683	Scanning electron microscopy (SEM) images	656:696	Scanning electron microscopy (SEM) images displayed the aggregations of BS in the cellulose surface.					
31362024	10	23	theme	shielding	1475:1483	arg1	glass					1485:1489	radiation shielding glass	1465:1489	radiation shielding glass	1465:1489	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	10	24	theme	shielding	1396:1404	arg1	material					1406:1413	X-ray radiation shielding material	1380:1413	X-ray radiation shielding material	1380:1413	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	10	24	theme	shielding	1396:1404	arg1	BSCM					1359:1362	the BSCM	1355:1362	the BSCM	1355:1362	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	1	25	theme	nuclear	191:197	arg1	fields					199:204	medical, aviation and nuclear fields	169:204	medical, aviation and nuclear fields	169:204	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	0	26	theme	X-ray	0:4	arg1	shielding					6:14	X-ray shielding structural	0:25	X-ray shielding structural	0:25	X-ray shielding structural and properties design for the porous transparent BaSO4/cellulose nanocomposite membranes.					
31362024	8	27	theme	mechanical	1182:1191	arg1	property					1193:1200	mechanical property	1182:1200	mechanical property	1182:1200	The swelling ratios, weight loss ratios and mechanical property were decreased along with the addition of BS.					
31362024	4	28	theme	electron	665:672	arg1	SEM					686:688	SEM	686:688	SEM	686:688	Scanning electron microscopy (SEM) images displayed the aggregations of BS in the cellulose surface.					
31362024	4	28	theme	electron	665:672	arg1	microscopy					674:683	Scanning electron microscopy	656:683	Scanning electron microscopy (SEM) images	656:696	Scanning electron microscopy (SEM) images displayed the aggregations of BS in the cellulose surface.					
31362024	3	29	theme	composite	575:583	arg1	membranes					585:593	composite membranes	575:593	composite membranes	575:593	Meanwhile, the influence of X-ray shielding capacity was studied by different layers of composite membranes and the shielding mechanism of the X-ray was also discussed.					
31362024	2	30	theme	addition	383:390	arg1	effects					343:349	The effects	339:349	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM)	339:466	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	2	31	theme	cellulose	444:452	arg1	membrane					454:461	the cellulose membrane	440:461	the cellulose membrane (CM)	440:466	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	2	31	theme	cellulose	444:452	arg1	CM					464:465	CM	464:465	CM	464:465	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	1	32	theme	porous	291:296	arg1	structure					310:318	porous transparent structure	291:318	porous transparent structure	291:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	5	33	dep	transform	765:773	arg1	infrared					775:782	infrared	775:782	transform infrared spectroscopy (FTIR)	765:802	Fourier transform infrared spectroscopy (FTIR) showed that the incorporation of BS into CM caused molecular interactions between CM and BS.					
31362024	3	34	theme	different	555:563	arg1	layers					565:570	different layers	555:570	different layers of composite membranes	555:593	Meanwhile, the influence of X-ray shielding capacity was studied by different layers of composite membranes and the shielding mechanism of the X-ray was also discussed.					
31362024	10	35	theme	radiation	1386:1394	arg1	material					1406:1413	X-ray radiation shielding material	1380:1413	X-ray radiation shielding material	1380:1413	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	10	35	theme	radiation	1386:1394	arg1	BSCM					1359:1362	the BSCM	1355:1362	the BSCM	1355:1362	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	0	36	theme	structural	16:25	arg1	shielding					6:14	X-ray shielding structural	0:25	X-ray shielding structural	0:25	X-ray shielding structural and properties design for the porous transparent BaSO4/cellulose nanocomposite membranes.					
31362024	1	37	theme	transparent	298:308	arg1	structure					310:318	porous transparent structure	291:318	porous transparent structure	291:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	10	38	theme	possible	1333:1340	arg1	work					1310:1313	this work	1305:1313	this work	1305:1313	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	10	38	theme	possible	1333:1340	arg1	example					1342:1348	a possible example	1331:1348	a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass	1331:1489	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	1	39	theme	effective	123:131	arg1	shielding					133:141	effective shielding	123:141	effective shielding of X-rays	123:151	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	5	40	theme	BS	837:838	arg1	incorporation					820:832	the incorporation	816:832	the incorporation of BS into CM	816:846	Fourier transform infrared spectroscopy (FTIR) showed that the incorporation of BS into CM caused molecular interactions between CM and BS.					
31362024	8	41	theme	weight	1159:1164	arg1	ratios					1171:1176	weight loss ratios	1159:1176	weight loss ratios	1159:1176	The swelling ratios, weight loss ratios and mechanical property were decreased along with the addition of BS.					
31362024	7	42	theme	water	1034:1038	arg1	WVTR					1066:1069	WVTR	1066:1069	WVTR	1066:1069	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	7	42	theme	water	1034:1038	arg1	Meanwhile					1019:1027	Meanwhile	1019:1027	Meanwhile	1019:1027	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	7	42	theme	water	1034:1038	arg1	rates					1059:1063	the water vapor transmission rates	1030:1063	the water vapor transmission rates (WVTR)	1030:1070	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	2	43	theme	morphological	412:424	arg1	properties					426:435	the physical and morphological properties	395:435	the physical and morphological properties of the cellulose membrane (CM)	395:466	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	4	44	theme	microscopy	674:683	arg1	images					691:696	Scanning electron microscopy (SEM) images	656:696	Scanning electron microscopy (SEM) images	656:696	Scanning electron microscopy (SEM) images displayed the aggregations of BS in the cellulose surface.					
31362024	1	45	theme	novel	209:213	arg1	material					277:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material	207:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure	207:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	2	46	theme	physical	399:406	arg1	properties					426:435	the physical and morphological properties	395:435	the physical and morphological properties of the cellulose membrane (CM)	395:466	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	5	47	dep	Fourier	757:763	arg1	transform					765:773	transform	765:773	transform infrared spectroscopy (FTIR)	765:802	Fourier transform infrared spectroscopy (FTIR) showed that the incorporation of BS into CM caused molecular interactions between CM and BS.					
31362024	6	48	theme	specific	982:989	arg1	area					999:1002	the specific surface area	978:1002	the specific surface area	978:1002	Brunauer-Emmett-Teller (BET) indicated that the load of BS contributed little to the specific surface area and pore size.					
31362024	3	49	theme	shielding	603:611	arg1	mechanism					613:621	the shielding mechanism	599:621	the shielding mechanism of the X-ray	599:634	Meanwhile, the influence of X-ray shielding capacity was studied by different layers of composite membranes and the shielding mechanism of the X-ray was also discussed.					
31362024	1	50	theme	X-ray	215:219	arg1	material					277:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material	207:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure	207:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	10	51	theme	radiation	1465:1473	arg1	glass					1485:1489	radiation shielding glass	1465:1489	radiation shielding glass	1465:1489	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	3	52	theme	membranes	585:593	arg1	layers					565:570	different layers	555:570	different layers of composite membranes	555:593	Meanwhile, the influence of X-ray shielding capacity was studied by different layers of composite membranes and the shielding mechanism of the X-ray was also discussed.					
31362024	1	53	with	material	277:284	arg1	structure					310:318	porous transparent structure	291:318	porous transparent structure	291:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	2	54	theme	carboxylated	354:365	arg1	addition					383:390	carboxylated nano-BaSO4 (BS) addition	354:390	carboxylated nano-BaSO4 (BS) addition	354:390	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	7	55	theme	vapor	1040:1044	arg1	WVTR					1066:1069	WVTR	1066:1069	WVTR	1066:1069	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	7	55	theme	vapor	1040:1044	arg1	Meanwhile					1019:1027	Meanwhile	1019:1027	Meanwhile	1019:1027	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	7	55	theme	vapor	1040:1044	arg1	rates					1059:1063	the water vapor transmission rates	1030:1063	the water vapor transmission rates (WVTR)	1030:1070	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	7	56	theme	transmission	1046:1057	arg1	WVTR					1066:1069	WVTR	1066:1069	WVTR	1066:1069	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	7	56	theme	transmission	1046:1057	arg1	Meanwhile					1019:1027	Meanwhile	1019:1027	Meanwhile	1019:1027	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	7	56	theme	transmission	1046:1057	arg1	rates					1059:1063	the water vapor transmission rates	1030:1063	the water vapor transmission rates (WVTR)	1030:1070	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	10	57	theme	glass	1485:1489	arg1	implication					1450:1460	the implication	1446:1460	the implication of radiation shielding glass	1446:1489	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	10	58	from	material	1406:1413	arg1	implication					1450:1460	the implication	1446:1460	the implication of radiation shielding glass	1446:1489	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	6	59	theme	pore	1008:1011	arg1	size					1013:1016	pore size	1008:1016	pore size	1008:1016	Brunauer-Emmett-Teller (BET) indicated that the load of BS contributed little to the specific surface area and pore size.					
31362024	1	60	theme	X-rays	146:151	arg1	shielding					133:141	effective shielding	123:141	effective shielding of X-rays	123:151	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	10	61	dep	example	1342:1348	arg1	designed					1368:1375	designed	1368:1375	was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass	1364:1489	Therefore, this work was regarded as a possible example that the BSCM was designed as X-ray radiation shielding material or sandwich filler material in the implication of radiation shielding glass.					
31362024	1	62	theme	shielding	221:229	arg1	material					277:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material	207:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure	207:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	3	63	theme	X-ray	630:634	arg1	mechanism					613:621	the shielding mechanism	599:621	the shielding mechanism of the X-ray	599:634	Meanwhile, the influence of X-ray shielding capacity was studied by different layers of composite membranes and the shielding mechanism of the X-ray was also discussed.					
31362024	3	64	theme	shielding	521:529	arg1	capacity					531:538	X-ray shielding capacity	515:538	X-ray shielding capacity	515:538	Meanwhile, the influence of X-ray shielding capacity was studied by different layers of composite membranes and the shielding mechanism of the X-ray was also discussed.					
31362024	1	65	theme	BaSO4/cellulose	231:245	arg1	material					277:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material	207:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure	207:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	0	66	theme	transparent	64:74	arg1	membranes					106:114	the porous transparent BaSO4/cellulose nanocomposite membranes	53:114	the porous transparent BaSO4/cellulose nanocomposite membranes	53:114	X-ray shielding structural and properties design for the porous transparent BaSO4/cellulose nanocomposite membranes.					
31362024	3	67	theme	X-ray	515:519	arg1	capacity					531:538	X-ray shielding capacity	515:538	X-ray shielding capacity	515:538	Meanwhile, the influence of X-ray shielding capacity was studied by different layers of composite membranes and the shielding mechanism of the X-ray was also discussed.					
31362024	5	68	theme	molecular	855:863	arg1	interactions					865:876	molecular interactions	855:876	molecular interactions between CM and BS	855:894	Fourier transform infrared spectroscopy (FTIR) showed that the incorporation of BS into CM caused molecular interactions between CM and BS.					
31362024	1	69	theme	nanocomposite	247:259	arg1	material					277:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material	207:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure	207:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
31362024	0	70	theme	porous	57:62	arg1	membranes					106:114	the porous transparent BaSO4/cellulose nanocomposite membranes	53:114	the porous transparent BaSO4/cellulose nanocomposite membranes	53:114	X-ray shielding structural and properties design for the porous transparent BaSO4/cellulose nanocomposite membranes.					
31362024	8	71	theme	swelling	1142:1149	arg1	ratios					1151:1156	The swelling ratios	1138:1156	The swelling ratios	1138:1156	The swelling ratios, weight loss ratios and mechanical property were decreased along with the addition of BS.					
31362024	2	72	theme	BS	379:380	arg1	addition					383:390	carboxylated nano-BaSO4 (BS) addition	354:390	carboxylated nano-BaSO4 (BS) addition	354:390	The effects of carboxylated nano-BaSO4 (BS) addition on the physical and morphological properties of the cellulose membrane (CM) were investigated.					
31362024	7	73	theme	BS	1134:1135	arg1	binding					1123:1129	the binding	1119:1129	the binding of BS	1119:1135	Meanwhile, the water vapor transmission rates (WVTR) also stayed at the same level before and after the binding of BS.					
31362024	1	74	theme	membrane	261:268	arg1	material					277:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material	207:284	a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure	207:318	Since effective shielding of X-rays was required in medical, aviation and nuclear fields, a novel X-ray shielding BaSO4/cellulose nanocomposite membrane (BSCM) material with porous transparent structure has been designed.					
30460692	10	0	theme	Activity	1561:1568	arg1	analysis					1570:1577	Activity analysis	1561:1577	Activity analysis of starch synthesis-related enzymes during the grain-filling stage	1561:1644	Activity analysis of starch synthesis-related enzymes during the grain-filling stage demonstrated the reliability of model fitting results.					
30460692	1	1	from	structure	302:310	arg1	rice					325:328	rice	325:328	rice	325:328	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	1	2	from	composition	286:296	arg1	rice					325:328	rice	325:328	rice	325:328	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	1	3	theme	High	169:172	arg1	temperature					174:184	BACKGROUND High temperature	158:184	BACKGROUND High temperature during the grain-filling stage	158:215	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	1	3	theme	High	169:172	arg1	factor					233:238	an important factor	220:238	an important factor that can affect grain quality by altering the composition and structure of starch in rice	220:328	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	6	4	theme	amylopectin	1149:1159	arg1	biosynthesis					1161:1172	the short and medium-long amylopectin biosynthesis	1123:1172	the short and medium-long amylopectin biosynthesis	1123:1172	In the non-waxy variety, the amylose biosynthesis may affect the short and medium-long amylopectin biosynthesis under high temperature.					
30460692	3	5	used	used	527:530	arg2	RESULTS					441:447	RESULTS	441:447	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055	441:520	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	10	6	theme	synthesis-related	1589:1605	arg1	enzymes					1607:1613	starch synthesis-related enzymes	1582:1613	starch synthesis-related enzymes during the grain-filling stage	1582:1644	Activity analysis of starch synthesis-related enzymes during the grain-filling stage demonstrated the reliability of model fitting results.					
30460692	1	7	theme	grain-filling	197:209	arg1	stage					211:215	the grain-filling stage	193:215	the grain-filling stage	193:215	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	10	8	theme	grain-filling	1626:1638	arg1	stage					1640:1644	the grain-filling stage	1622:1644	the grain-filling stage	1622:1644	Activity analysis of starch synthesis-related enzymes during the grain-filling stage demonstrated the reliability of model fitting results.					
30460692	6	9	theme	amylose	1091:1097	arg1	biosynthesis					1099:1110	the amylose biosynthesis	1087:1110	the amylose biosynthesis	1087:1110	In the non-waxy variety, the amylose biosynthesis may affect the short and medium-long amylopectin biosynthesis under high temperature.					
30460692	0	10	theme	integrated	127:136	arg1	Effects					0:6	Effects	0:6	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.	0:156	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	0	10	theme	integrated	127:136	arg1	analysis					148:155	integrated enzymatic analysis	127:155	integrated enzymatic analysis	127:155	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	3	11	theme	high	557:560	arg1	temperature					562:572	high temperature	557:572	high temperature	557:572	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	8	12	theme	high	1449:1452	arg1	temperature					1454:1464	high temperature	1449:1464	high temperature	1449:1464	The fitting results showed that the waxy and non-waxy rice had similar responses to high temperature.					
30460692	7	13	used	used	1231:1234	arg2	model					1221:1225	A mathematical fitting model	1198:1225	A mathematical fitting model	1198:1225	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	9	14	theme	parameter	1489:1497	arg1	response					1499:1506	the parameter response	1485:1506	the parameter response to high temperature	1485:1526	The variations of the parameter response to high temperature was more remarkable in Taihunuo.					
30460692	9	15	from	Taihunuo	1551:1558	arg1	remarkable					1537:1546	remarkable	1537:1546	remarkable	1537:1546	The variations of the parameter response to high temperature was more remarkable in Taihunuo.					
30460692	9	15	from	Taihunuo	1551:1558	arg1	variations					1471:1480	The variations	1467:1480	The variations of the parameter response to high temperature	1467:1526	The variations of the parameter response to high temperature was more remarkable in Taihunuo.					
30460692	4	16	theme	chain	657:661	arg1	distribution					670:681	starch chain length distribution	650:681	starch chain length distribution	650:681	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	3	17	from	effect	547:552	arg1	structure					586:594	the fine structure	577:594	the fine structure of starch	577:604	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	11	18	theme	fine	1754:1757	arg1	structure					1759:1767	the fine structure	1750:1767	the fine structure of starch	1750:1777	CONCLUSION The influences of high temperature on the fine structure of starch are similar between waxy and non-waxy rice.					
30460692	3	19	theme	fine	581:584	arg1	structure					586:594	the fine structure	577:594	the fine structure of starch	577:604	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	9	20	from	remarkable	1537:1546	arg1	Taihunuo					1551:1558	Taihunuo	1551:1558	Taihunuo	1551:1558	The variations of the parameter response to high temperature was more remarkable in Taihunuo.					
30460692	1	21	dep	composition	286:296	arg1	the					282:284	the	282:284	the	282:284	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	4	22	theme	distribution	670:681	arg1	Analysis					638:645	Analysis	638:645	Analysis of starch chain length distribution	638:681	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	5	23	theme	temperature	1038:1048	arg1	conditions					1050:1059	high temperature conditions	1033:1059	high temperature conditions	1033:1059	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice can shed light on the interactions of amylose and amylopectin synthesis under high temperature conditions.					
30460692	10	24	theme	results	1692:1698	arg1	reliability					1663:1673	the reliability	1659:1673	the reliability of model fitting results	1659:1698	Activity analysis of starch synthesis-related enzymes during the grain-filling stage demonstrated the reliability of model fitting results.					
30460692	5	25	theme	synthesis	883:891	arg1	differences					856:866	The differences	852:866	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice	852:953	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice can shed light on the interactions of amylose and amylopectin synthesis under high temperature conditions.					
30460692	0	26	from	Effects	0:6	arg1	structure					40:48	the fine structure	31:48	the fine structure of starch during the grain-filling stages in rice	31:98	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	0	27	theme	grain-filling	71:83	arg1	stages					85:90	the grain-filling stages	67:90	the grain-filling stages in rice	67:98	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	6	28	theme	high	1180:1183	arg1	temperature					1185:1195	high temperature	1180:1195	high temperature	1180:1195	In the non-waxy variety, the amylose biosynthesis may affect the short and medium-long amylopectin biosynthesis under high temperature.					
30460692	11	29	from	influences	1716:1725	arg1	structure					1759:1767	the fine structure	1750:1767	the fine structure of starch	1750:1777	CONCLUSION The influences of high temperature on the fine structure of starch are similar between waxy and non-waxy rice.					
30460692	3	30	theme	japonica	453:460	arg1	Taihunuo					487:494	the waxy rice Taihunuo	473:494	the waxy rice Taihunuo	473:494	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	3	30	theme	japonica	453:460	arg1	cultivars					462:470	Two japonica cultivars	449:470	Two japonica cultivars	449:470	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	3	30	theme	japonica	453:460	arg1	5055					517:520	non-waxy Nangeng 5055	500:520	non-waxy Nangeng 5055	500:520	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	4	31	theme	starch	754:759	arg1	content					743:749	the content	739:749	the content of starch	739:759	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	2	32	theme	regulatory	371:380	arg1	mechanism					382:390	the regulatory mechanism	367:390	the regulatory mechanism of high temperature on rice starch biosynthesis	367:438	Therefore, it is important to study the regulatory mechanism of high temperature on rice starch biosynthesis.					
30460692	4	33	from	chains	821:826	arg1	varieties					841:849	both rice varieties	831:849	both rice varieties	831:849	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	4	34	theme	high	712:715	arg1	temperature					717:727	a high temperature	710:727	a high temperature	710:727	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	2	35	theme	rice	415:418	arg1	biosynthesis					427:438	rice starch biosynthesis	415:438	rice starch biosynthesis	415:438	Therefore, it is important to study the regulatory mechanism of high temperature on rice starch biosynthesis.					
30460692	8	36	theme	fitting	1369:1375	arg1	results					1377:1383	The fitting results	1365:1383	The fitting results	1365:1383	The fitting results showed that the waxy and non-waxy rice had similar responses to high temperature.					
30460692	2	37	theme	temperature	400:410	arg1	mechanism					382:390	the regulatory mechanism	367:390	the regulatory mechanism of high temperature on rice starch biosynthesis	367:438	Therefore, it is important to study the regulatory mechanism of high temperature on rice starch biosynthesis.					
30460692	0	38	theme	high	11:14	arg1	temperature					16:26	high temperature	11:26	high temperature	11:26	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	5	39	theme	non-waxy	941:948	arg1	rice					950:953	non-waxy rice	941:953	non-waxy rice	941:953	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice can shed light on the interactions of amylose and amylopectin synthesis under high temperature conditions.					
30460692	3	40	theme	grain-filling	617:629	arg1	stage					631:635	the grain-filling stage	613:635	the grain-filling stage	613:635	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	10	41	theme	fitting	1684:1690	arg1	results					1692:1698	model fitting results	1678:1698	model fitting results	1678:1698	Activity analysis of starch synthesis-related enzymes during the grain-filling stage demonstrated the reliability of model fitting results.					
30460692	7	42	dep	significance	1327:1338	arg1	γ					1347:1347	γ	1347:1347	γ	1347:1347	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	7	42	dep	significance	1327:1338	arg1	β					1341:1341	β	1341:1341	β	1341:1341	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	0	43	theme	fine	35:38	arg1	structure					40:48	the fine structure	31:48	the fine structure of starch during the grain-filling stages in rice	31:98	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	12	44	theme	amylopectin	1855:1865	arg1	biosynthesis					1867:1878	amylopectin biosynthesis	1855:1878	amylopectin biosynthesis	1855:1878	Amylose biosynthesis may affect amylopectin biosynthesis under high temperature.					
30460692	5	45	theme	synthesis	1017:1025	arg1	interactions					977:988	the interactions	973:988	the interactions of amylose and amylopectin synthesis	973:1025	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice can shed light on the interactions of amylose and amylopectin synthesis under high temperature conditions.					
30460692	4	46	theme	medium-long	766:776	arg1	chains					778:783	medium-long chains	766:783	medium-long chains	766:783	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	11	47	dep	CONCLUSION	1701:1710	arg1	influences					1716:1725	The influences	1712:1725	The influences of high temperature on the fine structure of starch	1712:1777	CONCLUSION The influences of high temperature on the fine structure of starch are similar between waxy and non-waxy rice.					
30460692	11	47	dep	CONCLUSION	1701:1710	arg1	similar					1783:1789	similar	1783:1789	similar	1783:1789	CONCLUSION The influences of high temperature on the fine structure of starch are similar between waxy and non-waxy rice.					
30460692	3	48	dep	RESULTS	441:447	arg1	Taihunuo					487:494	the waxy rice Taihunuo	473:494	the waxy rice Taihunuo	473:494	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	3	48	dep	RESULTS	441:447	arg1	cultivars					462:470	Two japonica cultivars	449:470	Two japonica cultivars	449:470	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	3	48	dep	RESULTS	441:447	arg1	5055					517:520	non-waxy Nangeng 5055	500:520	non-waxy Nangeng 5055	500:520	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	7	49	theme	amylopectin	1271:1281	arg1	structure					1258:1266	the fine structure	1249:1266	the fine structure of amylopectin	1249:1281	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	11	50	theme	temperature	1735:1745	arg1	influences					1716:1725	The influences	1712:1725	The influences of high temperature on the fine structure of starch	1712:1777	CONCLUSION The influences of high temperature on the fine structure of starch are similar between waxy and non-waxy rice.					
30460692	11	50	theme	temperature	1735:1745	arg1	similar					1783:1789	similar	1783:1789	similar	1783:1789	CONCLUSION The influences of high temperature on the fine structure of starch are similar between waxy and non-waxy rice.					
30460692	0	51	dep	Effects	0:6	arg1	modeling					114:121	mathematical modeling	101:121	mathematical modeling	101:121	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	0	51	dep	Effects	0:6	arg1	Effects					0:6	Effects	0:6	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.	0:156	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	0	51	dep	Effects	0:6	arg1	analysis					148:155	integrated enzymatic analysis	127:155	integrated enzymatic analysis	127:155	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	3	52	theme	rice	482:485	arg1	Taihunuo					487:494	the waxy rice Taihunuo	473:494	the waxy rice Taihunuo	473:494	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	3	52	theme	rice	482:485	arg1	cultivars					462:470	Two japonica cultivars	449:470	Two japonica cultivars	449:470	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	4	53	theme	rice	836:839	arg1	varieties					841:849	both rice varieties	831:849	both rice varieties	831:849	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	8	54	contain	had	1424:1426	arg1	rice					1419:1422	non-waxy rice	1410:1422	non-waxy rice	1410:1422	The fitting results showed that the waxy and non-waxy rice had similar responses to high temperature.					
30460692	8	54	contain	had	1424:1426	arg1	waxy					1401:1404	waxy	1401:1404	waxy	1401:1404	The fitting results showed that the waxy and non-waxy rice had similar responses to high temperature.					
30460692	8	54	contain	had	1424:1426	arg2	responses					1436:1444	similar responses	1428:1444	similar responses to high temperature	1428:1464	The fitting results showed that the waxy and non-waxy rice had similar responses to high temperature.					
30460692	1	55	theme	grain	256:260	arg1	quality					262:268	grain quality	256:268	grain quality	256:268	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	9	56	theme	high	1511:1514	arg1	temperature					1516:1526	high temperature	1511:1526	high temperature	1511:1526	The variations of the parameter response to high temperature was more remarkable in Taihunuo.					
30460692	3	57	theme	Nangeng	509:515	arg1	cultivars					462:470	Two japonica cultivars	449:470	Two japonica cultivars	449:470	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	3	57	theme	Nangeng	509:515	arg1	5055					517:520	non-waxy Nangeng 5055	500:520	non-waxy Nangeng 5055	500:520	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	1	58	theme	BACKGROUND	158:167	arg1	temperature					174:184	BACKGROUND High temperature	158:184	BACKGROUND High temperature during the grain-filling stage	158:215	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	1	58	theme	BACKGROUND	158:167	arg1	factor					233:238	an important factor	220:238	an important factor that can affect grain quality by altering the composition and structure of starch in rice	220:328	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	4	59	theme	short	815:819	arg1	chains					821:826	short chains	815:826	short chains in both rice varieties	815:849	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	10	60	theme	enzymes	1607:1613	arg1	analysis					1570:1577	Activity analysis	1561:1577	Activity analysis of starch synthesis-related enzymes during the grain-filling stage	1561:1644	Activity analysis of starch synthesis-related enzymes during the grain-filling stage demonstrated the reliability of model fitting results.					
30460692	6	61	theme	medium-long	1137:1147	arg1	biosynthesis					1161:1172	the short and medium-long amylopectin biosynthesis	1123:1172	the short and medium-long amylopectin biosynthesis	1123:1172	In the non-waxy variety, the amylose biosynthesis may affect the short and medium-long amylopectin biosynthesis under high temperature.					
30460692	10	62	theme	starch	1582:1587	arg1	enzymes					1607:1613	starch synthesis-related enzymes	1582:1613	starch synthesis-related enzymes during the grain-filling stage	1582:1644	Activity analysis of starch synthesis-related enzymes during the grain-filling stage demonstrated the reliability of model fitting results.					
30460692	0	63	theme	mathematical	101:112	arg1	modeling					114:121	mathematical modeling	101:121	mathematical modeling	101:121	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	0	63	theme	mathematical	101:112	arg1	Effects					0:6	Effects	0:6	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.	0:156	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	8	64	theme	similar	1428:1434	arg1	responses					1436:1444	similar responses	1428:1444	similar responses to high temperature	1428:1464	The fitting results showed that the waxy and non-waxy rice had similar responses to high temperature.					
30460692	6	65	theme	short	1127:1131	arg1	biosynthesis					1161:1172	the short and medium-long amylopectin biosynthesis	1123:1172	the short and medium-long amylopectin biosynthesis	1123:1172	In the non-waxy variety, the amylose biosynthesis may affect the short and medium-long amylopectin biosynthesis under high temperature.					
30460692	7	66	with	parameters	1299:1308	arg1	significance					1327:1338	enzymatical significance	1315:1338	enzymatical significance (β and γ)	1315:1348	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	3	67	theme	temperature	562:572	arg1	effect					547:552	the effect	543:552	the effect of high temperature on the fine structure of starch	543:604	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	0	68	theme	enzymatic	138:146	arg1	Effects					0:6	Effects	0:6	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.	0:156	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	0	68	theme	enzymatic	138:146	arg1	analysis					148:155	integrated enzymatic analysis	127:155	integrated enzymatic analysis	127:155	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	5	69	theme	high	1033:1036	arg1	conditions					1050:1059	high temperature conditions	1033:1059	high temperature conditions	1033:1059	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice can shed light on the interactions of amylose and amylopectin synthesis under high temperature conditions.					
30460692	5	70	theme	high	907:910	arg1	temperature					912:922	high temperature	907:922	high temperature	907:922	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice can shed light on the interactions of amylose and amylopectin synthesis under high temperature conditions.					
30460692	0	71	from	stages	85:90	arg1	rice					95:98	rice	95:98	rice	95:98	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	11	72	theme	starch	1772:1777	arg1	structure					1759:1767	the fine structure	1750:1767	the fine structure of starch	1750:1777	CONCLUSION The influences of high temperature on the fine structure of starch are similar between waxy and non-waxy rice.					
30460692	0	73	theme	starch	53:58	arg1	structure					40:48	the fine structure	31:48	the fine structure of starch during the grain-filling stages in rice	31:98	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	11	74	dep	waxy	1799:1802	arg1	rice					1817:1820	rice	1817:1820	rice	1817:1820	CONCLUSION The influences of high temperature on the fine structure of starch are similar between waxy and non-waxy rice.					
30460692	9	75	theme	response	1499:1506	arg1	remarkable					1537:1546	remarkable	1537:1546	remarkable	1537:1546	The variations of the parameter response to high temperature was more remarkable in Taihunuo.					
30460692	9	75	theme	response	1499:1506	arg1	variations					1471:1480	The variations	1467:1480	The variations of the parameter response to high temperature	1467:1526	The variations of the parameter response to high temperature was more remarkable in Taihunuo.					
30460692	4	76	theme	starch	650:655	arg1	distribution					670:681	starch chain length distribution	650:681	starch chain length distribution	650:681	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	7	77	theme	fitting	1213:1219	arg1	model					1221:1225	A mathematical fitting model	1198:1225	A mathematical fitting model	1198:1225	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	5	78	theme	amylopectin	871:881	arg1	synthesis					883:891	amylopectin synthesis	871:891	amylopectin synthesis responding to high temperature	871:922	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice can shed light on the interactions of amylose and amylopectin synthesis under high temperature conditions.					
30460692	4	79	theme	length	663:668	arg1	distribution					670:681	starch chain length distribution	650:681	starch chain length distribution	650:681	Analysis of starch chain length distribution indicated that exposure to a high temperature increased the content of starch with medium-long chains and decreased the starch with short chains in both rice varieties.					
30460692	13	80	theme	Chemical	1922:1929	arg1	Industry					1931:1938	Chemical Industry	1922:1938	Chemical Industry	1922:1938	© 2018 Society of Chemical Industry.					
30460692	7	81	theme	mathematical	1200:1211	arg1	model					1221:1225	A mathematical fitting model	1198:1225	A mathematical fitting model	1198:1225	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	6	82	theme	non-waxy	1069:1076	arg1	variety					1078:1084	the non-waxy variety	1065:1084	the non-waxy variety	1065:1084	In the non-waxy variety, the amylose biosynthesis may affect the short and medium-long amylopectin biosynthesis under high temperature.					
30460692	7	83	theme	enzymatical	1315:1325	arg1	significance					1327:1338	enzymatical significance	1315:1338	enzymatical significance (β and γ)	1315:1348	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	2	84	theme	starch	420:425	arg1	biosynthesis					427:438	rice starch biosynthesis	415:438	rice starch biosynthesis	415:438	Therefore, it is important to study the regulatory mechanism of high temperature on rice starch biosynthesis.					
30460692	0	85	theme	temperature	16:26	arg1	modeling					114:121	mathematical modeling	101:121	mathematical modeling	101:121	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	0	85	theme	temperature	16:26	arg1	Effects					0:6	Effects	0:6	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.	0:156	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	0	85	theme	temperature	16:26	arg1	analysis					148:155	integrated enzymatic analysis	127:155	integrated enzymatic analysis	127:155	Effects of high temperature on the fine structure of starch during the grain-filling stages in rice: mathematical modeling and integrated enzymatic analysis.					
30460692	7	86	theme	parameters	1299:1308	arg1	series					1289:1294	a series	1287:1294	a series of parameters with enzymatical significance (β and γ)	1287:1348	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	2	87	theme	high	395:398	arg1	temperature					400:410	high temperature	395:410	high temperature	395:410	Therefore, it is important to study the regulatory mechanism of high temperature on rice starch biosynthesis.					
30460692	5	88	theme	amylose	993:999	arg1	interactions					977:988	the interactions	973:988	the interactions of amylose and amylopectin synthesis	973:1025	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice can shed light on the interactions of amylose and amylopectin synthesis under high temperature conditions.					
30460692	3	89	theme	starch	599:604	arg1	structure					586:594	the fine structure	577:594	the fine structure of starch	577:604	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	10	90	theme	model	1678:1682	arg1	results					1692:1698	model fitting results	1678:1698	model fitting results	1678:1698	Activity analysis of starch synthesis-related enzymes during the grain-filling stage demonstrated the reliability of model fitting results.					
30460692	5	91	theme	amylopectin	1005:1015	arg1	synthesis					1017:1025	amylopectin synthesis	1005:1025	amylopectin synthesis	1005:1025	The differences of amylopectin synthesis responding to high temperature between waxy and non-waxy rice can shed light on the interactions of amylose and amylopectin synthesis under high temperature conditions.					
30460692	12	92	theme	Amylose	1823:1829	arg1	biosynthesis					1831:1842	Amylose biosynthesis	1823:1842	Amylose biosynthesis	1823:1842	Amylose biosynthesis may affect amylopectin biosynthesis under high temperature.					
30460692	3	93	theme	waxy	477:480	arg1	Taihunuo					487:494	the waxy rice Taihunuo	473:494	the waxy rice Taihunuo	473:494	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	3	93	theme	waxy	477:480	arg1	cultivars					462:470	Two japonica cultivars	449:470	Two japonica cultivars	449:470	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	1	94	theme	starch	315:320	arg1	structure					302:310	structure	302:310	structure	302:310	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	1	94	theme	starch	315:320	arg1	composition					286:296	composition	286:296	composition	286:296	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	12	95	theme	high	1886:1889	arg1	temperature					1891:1901	high temperature	1886:1901	high temperature	1886:1901	Amylose biosynthesis may affect amylopectin biosynthesis under high temperature.					
30460692	11	96	theme	high	1730:1733	arg1	temperature					1735:1745	high temperature	1730:1745	high temperature	1730:1745	CONCLUSION The influences of high temperature on the fine structure of starch are similar between waxy and non-waxy rice.					
30460692	1	97	theme	important	223:231	arg1	factor					233:238	an important factor	220:238	an important factor that can affect grain quality by altering the composition and structure of starch in rice	220:328	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	1	97	theme	important	223:231	arg1	temperature					174:184	BACKGROUND High temperature	158:184	BACKGROUND High temperature during the grain-filling stage	158:215	BACKGROUND High temperature during the grain-filling stage is an important factor that can affect grain quality by altering the composition and structure of starch in rice.					
30460692	2	98	from	mechanism	382:390	arg1	biosynthesis					427:438	rice starch biosynthesis	415:438	rice starch biosynthesis	415:438	Therefore, it is important to study the regulatory mechanism of high temperature on rice starch biosynthesis.					
30460692	7	99	theme	fine	1253:1256	arg1	structure					1258:1266	the fine structure	1249:1266	the fine structure of amylopectin	1249:1281	A mathematical fitting model was used to interpret the fine structure of amylopectin and a series of parameters with enzymatical significance (β and γ) were obtained.					
30460692	8	100	theme	non-waxy	1410:1417	arg1	rice					1419:1422	non-waxy rice	1410:1422	non-waxy rice	1410:1422	The fitting results showed that the waxy and non-waxy rice had similar responses to high temperature.					
30460692	3	101	theme	non-waxy	500:507	arg1	cultivars					462:470	Two japonica cultivars	449:470	Two japonica cultivars	449:470	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
30460692	3	101	theme	non-waxy	500:507	arg1	5055					517:520	non-waxy Nangeng 5055	500:520	non-waxy Nangeng 5055	500:520	RESULTS Two japonica cultivars, the waxy rice Taihunuo and non-waxy Nangeng 5055 were used to examine the effect of high temperature on the fine structure of starch during the grain-filling stage.					
29605717	6	0	theme	different	942:950	arg1	fingerprints					962:973	significantly different molecular fingerprints	928:973	significantly different molecular fingerprints for collagen fibers	928:993	Screening of these regions with Raman microspectroscopy revealed significantly different molecular fingerprints for collagen fibers in colon carcinoma tissues compared to control tissues.					
29605717	12	1	theme	GAG	1923:1925	arg1	expression					1927:1936	GAG expression	1923:1936	GAG expression	1923:1936	To manipulate GAG expression and deposition in tumor microenvironments could represent a novel potential therapeutic strategy.					
29605717	4	2	theme	normal	579:584	arg1	tissues					602:608	normal (control) colon tissues	579:608	normal (control) colon tissues	579:608	In this study, ECM structures of human colon carcinoma and normal (control) colon tissues were histologically identified.					
29605717	5	3	theme	force	655:659	arg1	AFM					673:675	AFM	673:675	AFM	673:675	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	5	3	theme	force	655:659	arg1	microscopy					661:670	atomic force microscopy	648:670	atomic force microscopy (AFM) nanoindentation	648:692	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	7	4	theme	collagen	1167:1174	arg1	network					1176:1182	the collagen network	1163:1182	the collagen network of colon carcinoma tissues	1163:1209	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	4	5	theme	control	587:593	arg1	tissues					602:608	normal (control) colon tissues	579:608	normal (control) colon tissues	579:608	In this study, ECM structures of human colon carcinoma and normal (control) colon tissues were histologically identified.					
29605717	10	6	theme	tissues	1695:1701	arg1	composition					1625:1635	ECM composition	1621:1635	ECM composition	1621:1635	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	6	theme	tissues	1695:1701	arg1	biomechanics					1652:1663	biomechanics	1652:1663	biomechanics	1652:1663	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	6	theme	tissues	1695:1701	arg1	structure					1638:1646	structure	1638:1646	structure	1638:1646	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	9	7	theme	corresponding	1523:1535	arg1	tissues					1545:1551	the corresponding control tissues	1519:1551	the corresponding control tissues	1519:1551	Moreover, immunodetection of the collagen-associated proteoglycan decorin was significantly decreased in carcinomas tissues of individual patients when compared with the corresponding control tissues.					
29605717	6	8	theme	Raman	895:899	arg1	microspectroscopy					901:917	Raman microspectroscopy	895:917	Raman microspectroscopy	895:917	Screening of these regions with Raman microspectroscopy revealed significantly different molecular fingerprints for collagen fibers in colon carcinoma tissues compared to control tissues.					
29605717	8	9	theme	elevated	1296:1303	arg1	levels					1305:1310	significantly elevated levels	1282:1310	significantly elevated levels in collagen fibers of carcinoma tissues	1282:1350	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	10	10	theme	colon	1679:1683	arg1	tissues					1695:1701	individual colon carcinoma tissues	1668:1701	individual colon carcinoma tissues	1668:1701	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	11	11	theme	GAG	1787:1789	arg1	content					1791:1797	GAG content	1787:1797	GAG content	1787:1797	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	7	12	theme	immuno-reactivity	1138:1154	arg1	alignment					1082:1090	an increased alignment	1069:1090	an increased alignment of collagen fibers	1069:1109	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	7	12	theme	immuno-reactivity	1138:1154	arg1	levels					1124:1129	elevated levels	1115:1129	elevated levels of GAG immuno-reactivity	1115:1154	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	6	13	theme	regions	882:888	arg1	Screening					863:871	Screening	863:871	Screening of these regions with Raman microspectroscopy	863:917	Screening of these regions with Raman microspectroscopy revealed significantly different molecular fingerprints for collagen fibers in colon carcinoma tissues compared to control tissues.					
29605717	2	14	theme	tissue	331:336	arg1	stiffness					338:346	an increased tissue stiffness	318:346	an increased tissue stiffness	318:346	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	8	15	theme	chondroitin	1245:1255	arg1	sulfate					1257:1263	chondroitin sulfate	1245:1263	chondroitin sulfate	1245:1263	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	6	16	theme	carcinoma	1004:1012	arg1	tissues					1014:1020	colon carcinoma tissues	998:1020	colon carcinoma tissues	998:1020	Screening of these regions with Raman microspectroscopy revealed significantly different molecular fingerprints for collagen fibers in colon carcinoma tissues compared to control tissues.					
29605717	7	17	theme	elevated	1115:1122	arg1	levels					1124:1129	elevated levels	1115:1129	elevated levels of GAG immuno-reactivity	1115:1154	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	8	18	theme	carcinoma	1334:1342	arg1	tissues					1344:1350	carcinoma tissues	1334:1350	carcinoma tissues	1334:1350	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	7	19	theme	fibers	1104:1109	arg1	alignment					1082:1090	an increased alignment	1069:1090	an increased alignment of collagen fibers	1069:1109	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	7	19	theme	fibers	1104:1109	arg1	levels					1124:1129	elevated levels	1115:1129	elevated levels of GAG immuno-reactivity	1115:1154	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	5	20	theme	tissues	854:860	arg1	layer					837:841	the submucosal collagen-rich layer	808:841	the submucosal collagen-rich layer of control tissues	808:860	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	6	21	theme	collagen	979:986	arg1	fibers					988:993	collagen fibers	979:993	collagen fibers	979:993	Screening of these regions with Raman microspectroscopy revealed significantly different molecular fingerprints for collagen fibers in colon carcinoma tissues compared to control tissues.					
29605717	7	22	theme	increased	1072:1080	arg1	alignment					1082:1090	an increased alignment	1069:1090	an increased alignment of collagen fibers	1069:1109	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	2	23	theme	major	276:280	arg1	Overproduction					184:197	Overproduction	184:197	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers	184:270	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	2	23	theme	major	276:280	arg1	processes					282:290	major processes	276:290	major processes that ultimately result in an increased tissue stiffness	276:346	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	5	24	theme	collagen-rich	823:835	arg1	layer					837:841	the submucosal collagen-rich layer	808:841	the submucosal collagen-rich layer of control tissues	808:860	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	11	25	theme	ECM	1904:1906	arg1	remodeling					1871:1880	the mechanical and structural remodeling	1841:1880	the mechanical and structural remodeling of pathological tumor ECM	1841:1906	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	0	26	theme	colon	92:96	arg1	carcinomas					98:107	human colon carcinomas	86:107	human colon carcinomas	86:107	Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.					
29605717	1	27	theme	tumor	169:173	arg1	tissues					175:181	tumor tissues	169:181	tumor tissues	169:181	The extracellular matrix (ECM) is extensively remodeled in tumor tissues.					
29605717	6	28	theme	control	1034:1040	arg1	tissues					1042:1048	control tissues	1034:1048	control tissues	1034:1048	Screening of these regions with Raman microspectroscopy revealed significantly different molecular fingerprints for collagen fibers in colon carcinoma tissues compared to control tissues.					
29605717	0	29	from	Biomechanical	0:12	arg1	carcinomas					98:107	human colon carcinomas	86:107	human colon carcinomas	86:107	Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.					
29605717	11	30	theme	pathological	1885:1896	arg1	ECM					1904:1906	pathological tumor ECM	1885:1906	pathological tumor ECM	1885:1906	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	5	31	theme	tissues	768:774	arg1	ECM					745:747	the ECM	741:747	the ECM of colon carcinoma tissues	741:774	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	10	32	theme	strong	1564:1569	arg1	evident					1606:1612	evident	1606:1612	evident	1606:1612	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	32	theme	strong	1564:1569	arg1	variability					1590:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	33	dep	composition	1625:1635	arg1	the					1617:1619	the	1617:1619	the	1617:1619	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	9	34	theme	proteoglycan	1406:1417	arg1	decorin					1419:1425	the collagen-associated proteoglycan decorin	1382:1425	the collagen-associated proteoglycan decorin	1382:1425	Moreover, immunodetection of the collagen-associated proteoglycan decorin was significantly decreased in carcinomas tissues of individual patients when compared with the corresponding control tissues.					
29605717	2	35	theme	fibers	265:270	arg1	alignment					252:260	alignment	252:260	alignment of fibers	252:270	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	2	35	theme	fibers	265:270	arg1	crosslinking					235:246	pathological collagen crosslinking	213:246	pathological collagen crosslinking	213:246	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	2	35	theme	fibers	265:270	arg1	collagens					202:210	collagens	202:210	collagens	202:210	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	3	36	theme	biomechanical	470:482	arg1	properties					484:493	the biomechanical properties	466:493	the biomechanical properties of tumor ECM	466:506	Although it is known that glycosaminoglycans (GAGs) play an important role in tumor signaling, their contribution to the biomechanical properties of tumor ECM is unknown.					
29605717	0	37	theme	biomolecular	18:29	arg1	characterization					31:46	biomolecular characterization	18:46	biomolecular characterization	18:46	Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.					
29605717	12	38	theme	novel	1998:2002	arg1	strategy					2026:2033	a novel potential therapeutic strategy	1996:2033	a novel potential therapeutic strategy	1996:2033	To manipulate GAG expression and deposition in tumor microenvironments could represent a novel potential therapeutic strategy.					
29605717	10	39	from	biomechanics	1652:1663	arg1	evident					1606:1612	evident	1606:1612	evident	1606:1612	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	39	from	biomechanics	1652:1663	arg1	variability					1590:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	4	40	theme	colon	559:563	arg1	carcinoma					565:573	human colon carcinoma	553:573	human colon carcinoma	553:573	In this study, ECM structures of human colon carcinoma and normal (control) colon tissues were histologically identified.					
29605717	11	41	theme	important	1819:1827	arg1	role					1829:1832	an important role	1816:1832	an important role	1816:1832	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	7	42	theme	tissues	1203:1209	arg1	network					1176:1182	the collagen network	1163:1182	the collagen network of colon carcinoma tissues	1163:1209	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	12	43	theme	therapeutic	2014:2024	arg1	strategy					2026:2033	a novel potential therapeutic strategy	1996:2033	a novel potential therapeutic strategy	1996:2033	To manipulate GAG expression and deposition in tumor microenvironments could represent a novel potential therapeutic strategy.					
29605717	3	44	theme	important	409:417	arg1	role					419:422	an important role	406:422	an important role	406:422	Although it is known that glycosaminoglycans (GAGs) play an important role in tumor signaling, their contribution to the biomechanical properties of tumor ECM is unknown.					
29605717	3	45	theme	ECM	504:506	arg1	properties					484:493	the biomechanical properties	466:493	the biomechanical properties of tumor ECM	466:506	Although it is known that glycosaminoglycans (GAGs) play an important role in tumor signaling, their contribution to the biomechanical properties of tumor ECM is unknown.					
29605717	7	46	theme	colon	1187:1191	arg1	tissues					1203:1209	colon carcinoma tissues	1187:1209	colon carcinoma tissues	1187:1209	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	9	47	theme	carcinomas	1458:1467	arg1	tissues					1469:1475	carcinomas tissues	1458:1475	carcinomas tissues of individual patients	1458:1498	Moreover, immunodetection of the collagen-associated proteoglycan decorin was significantly decreased in carcinomas tissues of individual patients when compared with the corresponding control tissues.					
29605717	12	48	theme	tumor	1956:1960	arg1	microenvironments					1962:1978	tumor microenvironments	1956:1978	tumor microenvironments	1956:1978	To manipulate GAG expression and deposition in tumor microenvironments could represent a novel potential therapeutic strategy.					
29605717	4	49	theme	ECM	535:537	arg1	structures					539:548	ECM structures	535:548	ECM structures of human colon carcinoma and normal (control) colon tissues	535:608	In this study, ECM structures of human colon carcinoma and normal (control) colon tissues were histologically identified.					
29605717	2	50	theme	pathological	213:224	arg1	crosslinking					235:246	pathological collagen crosslinking	213:246	pathological collagen crosslinking	213:246	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	2	50	theme	pathological	213:224	arg1	collagens					202:210	collagens	202:210	collagens	202:210	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	10	51	theme	individual	1668:1677	arg1	tissues					1695:1701	individual colon carcinoma tissues	1668:1701	individual colon carcinoma tissues	1668:1701	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	2	52	theme	collagens	202:210	arg1	Overproduction					184:197	Overproduction	184:197	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers	184:270	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	2	52	theme	collagens	202:210	arg1	processes					282:290	major processes	276:290	major processes that ultimately result in an increased tissue stiffness	276:346	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	8	53	located	detected	1270:1277	arg1	levels					1305:1310	significantly elevated levels	1282:1310	significantly elevated levels in collagen fibers of carcinoma tissues	1282:1350	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	8	53	located	detected	1270:1277	arg2	GAGs					1212:1215	GAGs	1212:1215	GAGs such as heparan sulfate and chondroitin sulfate	1212:1263	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	8	53	located	detected	1270:1277	arg2	sulfate					1257:1263	chondroitin sulfate	1245:1263	chondroitin sulfate	1245:1263	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	8	53	located	detected	1270:1277	arg2	sulfate					1233:1239	heparan sulfate	1225:1239	heparan sulfate	1225:1239	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	9	54	theme	patients	1491:1498	arg1	tissues					1469:1475	carcinomas tissues	1458:1475	carcinomas tissues of individual patients	1458:1498	Moreover, immunodetection of the collagen-associated proteoglycan decorin was significantly decreased in carcinomas tissues of individual patients when compared with the corresponding control tissues.					
29605717	6	55	theme	molecular	952:960	arg1	fingerprints					962:973	significantly different molecular fingerprints	928:973	significantly different molecular fingerprints for collagen fibers	928:993	Screening of these regions with Raman microspectroscopy revealed significantly different molecular fingerprints for collagen fibers in colon carcinoma tissues compared to control tissues.					
29605717	4	56	theme	tissues	602:608	arg1	structures					539:548	ECM structures	535:548	ECM structures of human colon carcinoma and normal (control) colon tissues	535:608	In this study, ECM structures of human colon carcinoma and normal (control) colon tissues were histologically identified.					
29605717	5	57	theme	atomic	648:653	arg1	AFM					673:675	AFM	673:675	AFM	673:675	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	5	57	theme	atomic	648:653	arg1	microscopy					661:670	atomic force microscopy	648:670	atomic force microscopy (AFM) nanoindentation	648:692	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	11	58	theme	tumor	1747:1751	arg1	ECM					1753:1755	tumor ECM	1747:1755	tumor ECM	1747:1755	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	5	59	theme	microscopy	661:670	arg1	nanoindentation					678:692	atomic force microscopy (AFM) nanoindentation	648:692	atomic force microscopy (AFM) nanoindentation	648:692	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	10	60	theme	carcinoma	1685:1693	arg1	tissues					1695:1701	individual colon carcinoma tissues	1668:1701	individual colon carcinoma tissues	1668:1701	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	7	61	theme	GAG	1134:1136	arg1	immuno-reactivity					1138:1154	GAG immuno-reactivity	1134:1154	GAG immuno-reactivity	1134:1154	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	9	62	theme	control	1537:1543	arg1	tissues					1545:1551	the corresponding control tissues	1519:1551	the corresponding control tissues	1519:1551	Moreover, immunodetection of the collagen-associated proteoglycan decorin was significantly decreased in carcinomas tissues of individual patients when compared with the corresponding control tissues.					
29605717	0	63	theme	matrix	65:70	arg1	structures					72:81	extracellular matrix structures	51:81	extracellular matrix structures	51:81	Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.					
29605717	5	64	theme	carcinoma	758:766	arg1	tissues					768:774	colon carcinoma tissues	752:774	colon carcinoma tissues	752:774	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	8	65	theme	heparan	1225:1231	arg1	sulfate					1233:1239	heparan sulfate	1225:1239	heparan sulfate	1225:1239	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	2	66	theme	increased	321:329	arg1	stiffness					338:346	an increased tissue stiffness	318:346	an increased tissue stiffness	318:346	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	6	67	theme	colon	998:1002	arg1	tissues					1014:1020	colon carcinoma tissues	998:1020	colon carcinoma tissues	998:1020	Screening of these regions with Raman microspectroscopy revealed significantly different molecular fingerprints for collagen fibers in colon carcinoma tissues compared to control tissues.					
29605717	8	68	theme	collagen	1315:1322	arg1	fibers					1324:1329	collagen fibers	1315:1329	collagen fibers of carcinoma tissues	1315:1350	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	7	69	theme	collagen	1095:1102	arg1	fibers					1104:1109	collagen fibers	1095:1109	collagen fibers	1095:1109	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	5	70	theme	control	846:852	arg1	tissues					854:860	control tissues	846:860	control tissues	846:860	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	8	71	from	levels	1305:1310	arg1	fibers					1324:1329	collagen fibers	1315:1329	collagen fibers of carcinoma tissues	1315:1350	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	11	72	theme	mechanical	1845:1854	arg1	remodeling					1871:1880	the mechanical and structural remodeling	1841:1880	the mechanical and structural remodeling of pathological tumor ECM	1841:1906	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	8	73	theme	tissues	1344:1350	arg1	fibers					1324:1329	collagen fibers	1315:1329	collagen fibers of carcinoma tissues	1315:1350	GAGs such as heparan sulfate and chondroitin sulfate were detected in significantly elevated levels in collagen fibers of carcinoma tissues.					
29605717	0	74	theme	extracellular	51:63	arg1	structures					72:81	extracellular matrix structures	51:81	extracellular matrix structures	51:81	Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.					
29605717	10	75	from	evident	1606:1612	arg1	composition					1625:1635	ECM composition	1621:1635	ECM composition	1621:1635	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	75	from	evident	1606:1612	arg1	biomechanics					1652:1663	biomechanics	1652:1663	biomechanics	1652:1663	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	75	from	evident	1606:1612	arg1	structure					1638:1646	structure	1638:1646	structure	1638:1646	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	0	76	theme	human	86:90	arg1	carcinomas					98:107	human colon carcinomas	86:107	human colon carcinomas	86:107	Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.					
29605717	0	77	from	characterization	31:46	arg1	carcinomas					98:107	human colon carcinomas	86:107	human colon carcinomas	86:107	Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.					
29605717	11	78	theme	structural	1860:1869	arg1	remodeling					1871:1880	the mechanical and structural remodeling	1841:1880	the mechanical and structural remodeling of pathological tumor ECM	1841:1906	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	5	79	theme	colon	752:756	arg1	tissues					768:774	colon carcinoma tissues	752:774	colon carcinoma tissues	752:774	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	10	80	from	composition	1625:1635	arg1	evident					1606:1612	evident	1606:1612	evident	1606:1612	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	80	from	composition	1625:1635	arg1	variability					1590:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	5	81	theme	collagen-rich	712:724	arg1	stiffer					795:801	stiffer	795:801	stiffer	795:801	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	5	81	theme	collagen-rich	712:724	arg1	regions					726:732	the collagen-rich regions	708:732	the collagen-rich regions within the ECM of colon carcinoma tissues	708:774	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	10	82	from	structure	1638:1646	arg1	evident					1606:1612	evident	1606:1612	evident	1606:1612	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	82	from	structure	1638:1646	arg1	variability					1590:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	11	83	theme	tumor	1898:1902	arg1	ECM					1904:1906	pathological tumor ECM	1885:1906	pathological tumor ECM	1885:1906	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	6	84	with	Screening	863:871	arg1	microspectroscopy					901:917	Raman microspectroscopy	895:917	Raman microspectroscopy	895:917	Screening of these regions with Raman microspectroscopy revealed significantly different molecular fingerprints for collagen fibers in colon carcinoma tissues compared to control tissues.					
29605717	10	85	theme	patient-to-patient	1571:1588	arg1	evident					1606:1612	evident	1606:1612	evident	1606:1612	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	10	85	theme	patient-to-patient	1571:1588	arg1	variability					1590:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability	1554:1600	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	9	86	theme	collagen-associated	1386:1404	arg1	decorin					1419:1425	the collagen-associated proteoglycan decorin	1382:1425	the collagen-associated proteoglycan decorin	1382:1425	Moreover, immunodetection of the collagen-associated proteoglycan decorin was significantly decreased in carcinomas tissues of individual patients when compared with the corresponding control tissues.					
29605717	4	87	theme	human	553:557	arg1	carcinoma					565:573	human colon carcinoma	553:573	human colon carcinoma	553:573	In this study, ECM structures of human colon carcinoma and normal (control) colon tissues were histologically identified.					
29605717	5	88	theme	submucosal	812:821	arg1	layer					837:841	the submucosal collagen-rich layer	808:841	the submucosal collagen-rich layer of control tissues	808:860	Using atomic force microscopy (AFM) nanoindentation, we show that the collagen-rich regions within the ECM of colon carcinoma tissues were significantly stiffer than the submucosal collagen-rich layer of control tissues.					
29605717	9	89	theme	decorin	1419:1425	arg1	immunodetection					1363:1377	immunodetection	1363:1377	immunodetection of the collagen-associated proteoglycan decorin	1363:1425	Moreover, immunodetection of the collagen-associated proteoglycan decorin was significantly decreased in carcinomas tissues of individual patients when compared with the corresponding control tissues.					
29605717	12	90	theme	potential	2004:2012	arg1	strategy					2026:2033	a novel potential therapeutic strategy	1996:2033	a novel potential therapeutic strategy	1996:2033	To manipulate GAG expression and deposition in tumor microenvironments could represent a novel potential therapeutic strategy.					
29605717	1	91	theme	extracellular	114:126	arg1	ECM					136:138	ECM	136:138	ECM	136:138	The extracellular matrix (ECM) is extensively remodeled in tumor tissues.					
29605717	1	91	theme	extracellular	114:126	arg1	matrix					128:133	The extracellular matrix	110:133	The extracellular matrix (ECM)	110:139	The extracellular matrix (ECM) is extensively remodeled in tumor tissues.					
29605717	4	92	theme	carcinoma	565:573	arg1	structures					539:548	ECM structures	535:548	ECM structures of human colon carcinoma and normal (control) colon tissues	535:608	In this study, ECM structures of human colon carcinoma and normal (control) colon tissues were histologically identified.					
29605717	7	93	theme	carcinoma	1193:1201	arg1	tissues					1203:1209	colon carcinoma tissues	1187:1209	colon carcinoma tissues	1187:1209	We further showed an increased alignment of collagen fibers and elevated levels of GAG immuno-reactivity within the collagen network of colon carcinoma tissues.					
29605717	3	94	theme	tumor	427:431	arg1	signaling					433:441	tumor signaling	427:441	tumor signaling	427:441	Although it is known that glycosaminoglycans (GAGs) play an important role in tumor signaling, their contribution to the biomechanical properties of tumor ECM is unknown.					
29605717	11	95	theme	biomechanical	1714:1726	arg1	characteristics					1728:1742	biomechanical characteristics	1714:1742	biomechanical characteristics of tumor ECM	1714:1755	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	0	96	theme	structures	72:81	arg1	Biomechanical					0:12	Biomechanical	0:12	Biomechanical	0:12	Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.					
29605717	0	96	theme	structures	72:81	arg1	characterization					31:46	biomolecular characterization	18:46	biomolecular characterization	18:46	Biomechanical and biomolecular characterization of extracellular matrix structures in human colon carcinomas.					
29605717	2	97	theme	collagen	226:233	arg1	crosslinking					235:246	pathological collagen crosslinking	213:246	pathological collagen crosslinking	213:246	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	2	97	theme	collagen	226:233	arg1	collagens					202:210	collagens	202:210	collagens	202:210	Overproduction of collagens, pathological collagen crosslinking and alignment of fibers are major processes that ultimately result in an increased tissue stiffness.					
29605717	12	98	from	deposition	1942:1951	arg1	microenvironments					1962:1978	tumor microenvironments	1956:1978	tumor microenvironments	1956:1978	To manipulate GAG expression and deposition in tumor microenvironments could represent a novel potential therapeutic strategy.					
29605717	4	99	theme	colon	596:600	arg1	tissues					602:608	normal (control) colon tissues	579:608	normal (control) colon tissues	579:608	In this study, ECM structures of human colon carcinoma and normal (control) colon tissues were histologically identified.					
29605717	10	100	theme	ECM	1621:1623	arg1	composition					1625:1635	ECM composition	1621:1635	ECM composition	1621:1635	Overall a strong patient-to-patient variability was evident in the ECM composition, structure and biomechanics of individual colon carcinoma tissues.					
29605717	11	101	theme	ECM	1753:1755	arg1	characteristics					1728:1742	biomechanical characteristics	1714:1742	biomechanical characteristics of tumor ECM	1714:1755	Although, biomechanical characteristics of tumor ECM were not directly impacted by GAG content, GAGs might play an important role during the mechanical and structural remodeling of pathological tumor ECM.					
29605717	3	102	theme	tumor	498:502	arg1	ECM					504:506	tumor ECM	498:506	tumor ECM	498:506	Although it is known that glycosaminoglycans (GAGs) play an important role in tumor signaling, their contribution to the biomechanical properties of tumor ECM is unknown.					
29605717	9	103	theme	individual	1480:1489	arg1	patients					1491:1498	individual patients	1480:1498	individual patients	1480:1498	Moreover, immunodetection of the collagen-associated proteoglycan decorin was significantly decreased in carcinomas tissues of individual patients when compared with the corresponding control tissues.					
29605717	12	104	from	expression	1927:1936	arg1	microenvironments					1962:1978	tumor microenvironments	1956:1978	tumor microenvironments	1956:1978	To manipulate GAG expression and deposition in tumor microenvironments could represent a novel potential therapeutic strategy.					
30088442	2	0	theme	rheological	488:498	arg1	behavior					500:507	The rheological behavior	484:507	The rheological behavior of glycol chitin (7 wt%)/PAA	484:536	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	2	1	theme	i.e.	587:590	arg1	17 °C					592:596	i.e. 17 °C	587:596	i.e. 17 °C	587:596	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	1	2	theme	drug	191:194	arg1	system					211:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	3	3	theme	gelling	690:696	arg1	16 minutes					727:736	16 minutes	727:736	16 minutes	727:736	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	3	3	theme	gelling	690:696	arg1	time					698:701	The gelling time	686:701	The gelling time of glycol chitin/PAA	686:722	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	0	4	theme	sustained	100:108	arg1	release					110:116	sustained release	100:116	sustained release of anticancer drug through intratumoral injection	100:166	Glycol chitin/PAA hydrogel composite incorporated bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection.					
30088442	1	5	theme	-loaded	331:337	arg1	micro-particles					398:412	Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles	315:412	Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles	315:412	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	6	6	theme	in-situ	1232:1238	arg1	formulations					1244:1255	in-situ gel formulations	1232:1255	in-situ gel formulations	1232:1255	This system is a promising approach for cancer therapy through intratumoral injection of in-situ gel formulations to extend retention time at tumor sites.					
30088442	1	7	theme	release	196:202	arg1	system					211:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	5	8	contain	have	1047:1050	arg2	effect					1067:1072	62% inhibitory effect	1052:1072	62% inhibitory effect on hepatoma cells	1052:1090	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	5	8	contain	have	1047:1050	arg1	micro-particles					1015:1029	the micro-particles	1011:1029	the micro-particles with drug	1011:1039	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	0	9	theme	anticancer	121:130	arg1	drug					132:135	anticancer drug	121:135	anticancer drug	121:135	Glycol chitin/PAA hydrogel composite incorporated bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection.					
30088442	1	10	theme	bio-biofunctionalized	339:359	arg1	PLGA					392:395	PLGA	392:395	PLGA	392:395	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	1	10	theme	bio-biofunctionalized	339:359	arg1	poly					361:364	bio-biofunctionalized poly	339:364	Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles	315:412	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	1	10	theme	bio-biofunctionalized	339:359	arg1	acid					385:388	lactic-co-glycolic acid	366:388	lactic-co-glycolic acid	366:388	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	3	11	from	25 °C	741:745	arg1	16 minutes					727:736	16 minutes	727:736	16 minutes	727:736	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	3	11	from	25 °C	741:745	arg1	time					698:701	The gelling time	686:701	The gelling time of glycol chitin/PAA	686:722	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	1	12	with	combination	298:308	arg1	micro-particles					398:412	Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles	315:412	Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles	315:412	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	3	13	theme	gel	825:827	arg1	formulations					829:840	gel formulations	825:840	the in-situ gel formulations	813:840	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	4	14	theme	release	887:893	arg1	behavior					895:902	The drug release behavior	878:902	The drug release behavior	878:902	The drug release behavior reveals that the system can dramatically postpone the drug release.					
30088442	1	15	theme	poly	361:364	arg1	micro-particles					398:412	Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles	315:412	Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles	315:412	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	2	16	theme	gelling	566:572	arg1	temperature					574:584	low gelling temperature	562:584	low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition	562:683	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	1	17	theme	cancer	437:442	arg1	therapy					444:450	cancer therapy	437:450	cancer therapy	437:450	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	3	18	theme	patient	869:875	arg1	location					857:864	the tumor location	847:864	the tumor location of patient	847:875	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	1	19	theme	glycol	268:273	arg1	chitin					275:280	glycol chitin	268:280	glycol chitin	268:280	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	2	20	theme	chitin	519:524	arg1	/PAA					533:536	glycol chitin (7 wt%)/PAA	512:536	glycol chitin (7 wt%)/PAA	512:536	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	5	21	theme	62	1052:1053	arg1	%					1054:1054	%	1054:1054	%	1054:1054	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	2	22	theme	/PAA	533:536	arg1	behavior					500:507	The rheological behavior	484:507	The rheological behavior of glycol chitin (7 wt%)/PAA	484:536	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	1	23	theme	hybrid	204:209	arg1	system					211:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	0	24	theme	drug	132:135	arg1	release					110:116	sustained release	100:116	sustained release of anticancer drug through intratumoral injection	100:166	Glycol chitin/PAA hydrogel composite incorporated bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection.					
30088442	1	25	theme	lactic-co-glycolic	366:383	arg1	poly					361:364	bio-biofunctionalized poly	339:364	Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles	315:412	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	1	25	theme	lactic-co-glycolic	366:383	arg1	acid					385:388	lactic-co-glycolic acid	366:388	lactic-co-glycolic acid	366:388	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	2	26	dep	temperature	574:584	arg1	17 °C					592:596	i.e. 17 °C	587:596	i.e. 17 °C	587:596	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	0	27	theme	chitin/PAA	7:16	arg1	composite					27:35	Glycol chitin/PAA hydrogel composite	0:35	Glycol chitin/PAA hydrogel composite	0:35	Glycol chitin/PAA hydrogel composite incorporated bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection.					
30088442	5	28	theme	inhibitory	1056:1065	arg1	effect					1067:1072	62% inhibitory effect	1052:1072	62% inhibitory effect on hepatoma cells	1052:1090	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	1	29	theme	intratumoral	460:471	arg1	injection					473:481	intratumoral injection	460:481	intratumoral injection	460:481	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	3	30	theme	tumor	851:855	arg1	location					857:864	the tumor location	847:864	the tumor location of patient	847:875	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	2	31	from	condition	675:683	arg1	gel					654:656	gel	654:656	gel at physiological condition	654:683	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	0	32	theme	Glycol	0:5	arg1	composite					27:35	Glycol chitin/PAA hydrogel composite	0:35	Glycol chitin/PAA hydrogel composite	0:35	Glycol chitin/PAA hydrogel composite incorporated bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection.					
30088442	3	33	theme	chitin/PAA	713:722	arg1	16 minutes					727:736	16 minutes	727:736	16 minutes	727:736	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	3	33	theme	chitin/PAA	713:722	arg1	time					698:701	The gelling time	686:701	The gelling time of glycol chitin/PAA	686:722	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	6	34	theme	cancer	1183:1188	arg1	therapy					1190:1196	cancer therapy	1183:1196	cancer therapy	1183:1196	This system is a promising approach for cancer therapy through intratumoral injection of in-situ gel formulations to extend retention time at tumor sites.					
30088442	3	35	dep	formulations	829:840	arg1	in-situ					817:823	the in-situ gel formulations	813:840	the in-situ gel formulations	813:840	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	6	36	theme	intratumoral	1206:1217	arg1	injection					1219:1227	intratumoral injection	1206:1227	intratumoral injection of in-situ gel formulations	1206:1255	This system is a promising approach for cancer therapy through intratumoral injection of in-situ gel formulations to extend retention time at tumor sites.					
30088442	6	37	theme	tumor	1285:1289	arg1	sites					1291:1295	tumor sites	1285:1295	tumor sites	1285:1295	This system is a promising approach for cancer therapy through intratumoral injection of in-situ gel formulations to extend retention time at tumor sites.					
30088442	5	38	with	combing	1120:1126	arg1	hydrogel					1133:1140	hydrogel	1133:1140	hydrogel	1133:1140	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	5	39	with	micro-particles	1015:1029	arg1	drug					1036:1039	drug	1036:1039	drug	1036:1039	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	0	40	theme	hydrogel	18:25	arg1	composite					27:35	Glycol chitin/PAA hydrogel composite	0:35	Glycol chitin/PAA hydrogel composite	0:35	Glycol chitin/PAA hydrogel composite incorporated bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection.					
30088442	0	41	theme	intratumoral	145:156	arg1	injection					158:166	intratumoral injection	145:166	intratumoral injection	145:166	Glycol chitin/PAA hydrogel composite incorporated bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection.					
30088442	5	42	theme	%	1054:1054	arg1	effect					1067:1072	62% inhibitory effect	1052:1072	62% inhibitory effect on hepatoma cells	1052:1090	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	2	43	contain	has	558:560	arg1	it					555:556	it	555:556	it	555:556	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	2	43	contain	has	558:560	arg2	temperature					574:584	low gelling temperature	562:584	low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition	562:683	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	0	44	theme	bio-functionalized	50:67	arg1	microspheres					74:85	bio-functionalized PLGA microspheres	50:85	bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection	50:166	Glycol chitin/PAA hydrogel composite incorporated bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection.					
30088442	3	45	theme	glycol	706:711	arg1	chitin/PAA					713:722	glycol chitin/PAA	706:722	glycol chitin/PAA	706:722	The gelling time of glycol chitin/PAA is 16 minutes at 25 °C and 3 minutes at 37 °C, which is convenient for doctors to inject the in-situ gel formulations into the tumor location of patient.					
30088442	4	46	theme	drug	882:885	arg1	behavior					895:902	The drug release behavior	878:902	The drug release behavior	878:902	The drug release behavior reveals that the system can dramatically postpone the drug release.					
30088442	6	47	theme	gel	1240:1242	arg1	formulations					1244:1255	in-situ gel formulations	1232:1255	in-situ gel formulations	1232:1255	This system is a promising approach for cancer therapy through intratumoral injection of in-situ gel formulations to extend retention time at tumor sites.					
30088442	4	48	theme	drug	958:961	arg1	release					963:969	the drug release	954:969	the drug release	954:969	The drug release behavior reveals that the system can dramatically postpone the drug release.					
30088442	2	49	theme	%	531:531	arg1	/PAA					533:536	glycol chitin (7 wt%)/PAA	512:536	glycol chitin (7 wt%)/PAA	512:536	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	2	50	theme	glycol	512:517	arg1	/PAA					533:536	glycol chitin (7 wt%)/PAA	512:536	glycol chitin (7 wt%)/PAA	512:536	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	5	51	from	effect	1067:1072	arg1	cells					1086:1090	hepatoma cells	1077:1090	hepatoma cells	1077:1090	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	2	52	theme	7 wt	527:530	arg1	/PAA					533:536	glycol chitin (7 wt%)/PAA	512:536	glycol chitin (7 wt%)/PAA	512:536	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	2	53	theme	low	562:564	arg1	temperature					574:584	low gelling temperature	562:584	low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition	562:683	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	5	54	theme	cell	976:979	arg1	test					991:994	The cell viability test	972:994	The cell viability test	972:994	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	2	55	theme	physiological	661:673	arg1	condition					675:683	physiological condition	661:683	physiological condition	661:683	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	5	56	theme	hepatoma	1077:1084	arg1	cells					1086:1090	hepatoma cells	1077:1090	hepatoma cells	1077:1090	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	0	57	theme	PLGA	69:72	arg1	microspheres					74:85	bio-functionalized PLGA microspheres	50:85	bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection	50:166	Glycol chitin/PAA hydrogel composite incorporated bio-functionalized PLGA microspheres intended for sustained release of anticancer drug through intratumoral injection.					
30088442	5	58	theme	fourteenth	1099:1108	arg1	day					1110:1112	the fourteenth day	1095:1112	the fourteenth day after combing with hydrogel	1095:1140	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	5	59	theme	viability	981:989	arg1	test					991:994	The cell viability test	972:994	The cell viability test	972:994	The cell viability test indicates that the micro-particles with drug still have 62% inhibitory effect on hepatoma cells in the fourteenth day after combing with hydrogel.					
30088442	2	60	from	pH	601:602	arg1	17 °C					592:596	i.e. 17 °C	587:596	i.e. 17 °C	587:596	The rheological behavior of glycol chitin (7 wt%)/PAA illustrates that it has low gelling temperature (i.e. 17 °C at pH 7.56) which ensures that the formulation turns to gel at physiological condition.					
30088442	1	61	theme	acrylic	244:250	arg1	poly					239:242	poly	239:242	poly(acrylic acid) (PAA)	239:262	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	1	61	theme	acrylic	244:250	arg1	acid					252:255	acrylic acid	244:255	acrylic acid	244:255	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	6	62	theme	formulations	1244:1255	arg1	injection					1219:1227	intratumoral injection	1206:1227	intratumoral injection of in-situ gel formulations	1206:1255	This system is a promising approach for cancer therapy through intratumoral injection of in-situ gel formulations to extend retention time at tumor sites.					
30088442	6	63	theme	promising	1160:1168	arg1	system					1148:1153	This system	1143:1153	This system	1143:1153	This system is a promising approach for cancer therapy through intratumoral injection of in-situ gel formulations to extend retention time at tumor sites.					
30088442	6	63	theme	promising	1160:1168	arg1	approach					1170:1177	a promising approach	1158:1177	a promising approach for cancer therapy	1158:1196	This system is a promising approach for cancer therapy through intratumoral injection of in-situ gel formulations to extend retention time at tumor sites.					
30088442	1	64	theme	novel	171:175	arg1	system					211:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30088442	6	65	theme	retention	1267:1275	arg1	time					1277:1280	retention time	1267:1280	retention time	1267:1280	This system is a promising approach for cancer therapy through intratumoral injection of in-situ gel formulations to extend retention time at tumor sites.					
30088442	1	66	theme	anti-hepatoma	177:189	arg1	system					211:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system	169:216	A novel anti-hepatoma drug release hybrid system is prepared by using poly(acrylic acid) (PAA) and glycol chitin as substrate in combination with Paclitaxel (PTX)-loaded bio-biofunctionalized poly(lactic-co-glycolic acid) (PLGA) micro-particles, which is intended for cancer therapy through intratumoral injection.					
30395638	8	0	theme	sea	1613:1615	arg1	organisms					1617:1625	deep sea organisms	1608:1625	deep sea organisms	1608:1625	The remoteness of the deep sea does not prevent the accumulation of microfibres, being available to become integrated into deep sea organisms.					
30395638	3	1	theme	type	695:698	arg1	magnitude					677:685	magnitude	677:685	magnitude	677:685	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	3	1	theme	type	695:698	arg1	transport					663:671	long-range transport	652:671	long-range transport	652:671	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	4	2	from	ml	775:776	arg1	abundances					737:746	abundances	737:746	abundances	737:746	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	4	2	from	ml	775:776	arg1	natural					806:812	natural	806:812	natural	806:812	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	4	2	from	ml	775:776	arg1	cellulose					830:838	cellulose	830:838	cellulose	830:838	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	2	3	theme	particles	355:363	arg1	effects					331:337	effects	331:337	effects	331:337	However, relatively little attention has been paid to the fate and effects of microplastic particles that are fibrous in shape, also referred as microfibres, which are mostly shed from synthetic textiles during production or washing.					
30395638	2	3	theme	particles	355:363	arg1	fate					322:325	fate	322:325	fate	322:325	However, relatively little attention has been paid to the fate and effects of microplastic particles that are fibrous in shape, also referred as microfibres, which are mostly shed from synthetic textiles during production or washing.					
30395638	7	4	theme	microfibres	1401:1411	arg1	%					1392:1392	Around 20%	1383:1392	Around 20% of the microfibres found	1383:1417	Around 20% of the microfibres found had accumulated in the deep open sea beyond 2000m of water depth.					
30395638	7	4	theme	microfibres	1401:1411	arg1	microfibres					1401:1411	the microfibres	1397:1411	the microfibres found	1397:1417	Around 20% of the microfibres found had accumulated in the deep open sea beyond 2000m of water depth.					
30395638	0	5	from	imprint	4:10	arg1	seas					53:56	southern European deep seas	30:56	southern European deep seas	30:56	The imprint of microfibres in southern European deep seas.					
30395638	2	6	theme	microplastic	342:353	arg1	particles					355:363	microplastic particles	342:363	microplastic particles that are fibrous in shape, also referred as microfibres, which are mostly shed from synthetic textiles during production or washing	342:495	However, relatively little attention has been paid to the fate and effects of microplastic particles that are fibrous in shape, also referred as microfibres, which are mostly shed from synthetic textiles during production or washing.					
30395638	3	7	from	abundance	529:537	arg1	sediments					566:574	seafloor sediments	557:574	seafloor sediments in southern European seas	557:600	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	7	8	theme	water	1472:1476	arg1	depth					1478:1482	water depth	1472:1482	water depth	1472:1482	Around 20% of the microfibres found had accumulated in the deep open sea beyond 2000m of water depth.					
30395638	5	9	theme	floor	1096:1100	arg1	microfibres					1102:1112	the sea floor microfibres	1088:1112	the sea floor microfibres	1088:1112	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	6	10	theme	Submarine	1218:1226	arg1	conduits					1256:1263	preferential conduits	1243:1263	preferential conduits for downslope transport of microfibres	1243:1302	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	6	10	theme	Submarine	1218:1226	arg1	canyons					1228:1234	Submarine canyons	1218:1234	Submarine canyons	1218:1234	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	4	11	from	microfibres	757:767	arg1	ml					775:776	50 ml	772:776	50 ml of sediment	772:788	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	4	12	theme	microfibres	757:767	arg1	abundances					737:746	abundances	737:746	abundances	737:746	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	4	12	theme	microfibres	757:767	arg1	natural					806:812	natural	806:812	natural	806:812	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	4	12	theme	microfibres	757:767	arg1	cellulose					830:838	cellulose	830:838	cellulose	830:838	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	3	13	dep	transport	663:671	arg1	the					648:650	the	648:650	the	648:650	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	8	14	theme	sea	1512:1514	arg1	remoteness					1489:1498	The remoteness	1485:1498	The remoteness of the deep sea	1485:1514	The remoteness of the deep sea does not prevent the accumulation of microfibres, being available to become integrated into deep sea organisms.					
30395638	3	15	theme	pollution	716:724	arg1	type					695:698	this type	690:698	this type of microplastic pollution	690:724	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	1	16	from	fragments	128:136	arg1	organisms					169:177	organisms	169:177	organisms	169:177	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	4	17	dep	natural	806:812	arg1	fibres					928:933	fibres	928:933	fibres	928:933	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	2	18	theme	little	284:289	arg1	attention					291:299	relatively little attention	273:299	relatively little attention	273:299	However, relatively little attention has been paid to the fate and effects of microplastic particles that are fibrous in shape, also referred as microfibres, which are mostly shed from synthetic textiles during production or washing.					
30395638	8	19	theme	deep	1507:1510	arg1	sea					1512:1514	the deep sea	1503:1514	the deep sea	1503:1514	The remoteness of the deep sea does not prevent the accumulation of microfibres, being available to become integrated into deep sea organisms.					
30395638	5	20	theme	basin	965:969	arg1	approach					981:988	a shelf-slope-deep basin continuum approach	946:988	a shelf-slope-deep basin continuum approach	946:988	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	3	21	from	gaps	611:614	arg1	understanding					631:643	the limited understanding	619:643	the limited understanding of the long-range transport and magnitude of this type of microplastic pollution	619:724	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	6	22	theme	open	1371:1374	arg1	slope					1376:1380	the open slope	1367:1380	the open slope	1367:1380	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	3	23	theme	microplastic	703:714	arg1	pollution					716:724	microplastic pollution	703:724	microplastic pollution	703:724	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	4	24	from	abundances	737:746	arg1	ml					775:776	50 ml	772:776	50 ml of sediment	772:788	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	3	25	theme	seafloor	557:564	arg1	sediments					566:574	seafloor sediments	557:574	seafloor sediments in southern European seas	557:600	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	8	26	theme	deep	1608:1611	arg1	organisms					1617:1625	deep sea organisms	1608:1625	deep sea organisms	1608:1625	The remoteness of the deep sea does not prevent the accumulation of microfibres, being available to become integrated into deep sea organisms.					
30395638	3	27	theme	magnitude	677:685	arg1	understanding					631:643	the limited understanding	619:643	the limited understanding of the long-range transport and magnitude of this type of microplastic pollution	619:724	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	1	28	theme	marine	76:81	arg1	environment					83:93	the marine environment	72:93	the marine environment	72:93	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	5	29	theme	sea	1092:1094	arg1	microfibres					1102:1112	the sea floor microfibres	1088:1112	the sea floor microfibres	1088:1112	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	0	30	theme	microfibres	15:25	arg1	imprint					4:10	The imprint	0:10	The imprint of microfibres in southern European deep seas.	0:57	The imprint of microfibres in southern European deep seas.					
30395638	6	31	located	found	1358:1362	arg1	slope					1376:1380	the open slope	1367:1380	the open slope	1367:1380	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	6	31	located	found	1358:1362	arg2	%					1356:1356	18%	1354:1356	18% found on the open slope	1354:1380	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	1	32	theme	environment	83:93	arg1	Pollution					59:67	Pollution	59:67	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms	59:177	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	1	33	theme	stimulated	183:192	arg1	interest					216:223	stimulated considerable research interest	183:223	stimulated considerable research interest	183:223	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	2	34	dep	fate	322:325	arg1	the					318:320	the	318:320	the	318:320	However, relatively little attention has been paid to the fate and effects of microplastic particles that are fibrous in shape, also referred as microfibres, which are mostly shed from synthetic textiles during production or washing.					
30395638	5	35	theme	shelf-slope-deep	948:963	arg1	approach					981:988	a shelf-slope-deep basin continuum approach	946:988	a shelf-slope-deep basin continuum approach	946:988	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	1	36	contain	has	179:181	arg1	Pollution					59:67	Pollution	59:67	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms	59:177	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	1	36	contain	has	179:181	arg2	interest					216:223	stimulated considerable research interest	183:223	stimulated considerable research interest	183:223	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	1	37	theme	potential	148:156	arg1	impacts					158:164	their potential impacts	142:164	their potential impacts on organisms	142:177	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	1	38	theme	considerable	194:205	arg1	interest					216:223	stimulated considerable research interest	183:223	stimulated considerable research interest	183:223	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	0	39	theme	southern	30:37	arg1	seas					53:56	southern European deep seas	30:56	southern European deep seas	30:56	The imprint of microfibres in southern European deep seas.					
30395638	5	40	theme	fibres	1145:1150	arg1	quantities					1127:1136	greater quantities	1119:1136	greater quantities of the fibres	1119:1150	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	7	41	theme	open	1447:1450	arg1	sea					1452:1454	the deep open sea	1438:1454	the deep open sea beyond 2000m of water depth	1438:1482	Around 20% of the microfibres found had accumulated in the deep open sea beyond 2000m of water depth.					
30395638	3	42	theme	European	588:595	arg1	seas					597:600	southern European seas	579:600	southern European seas	579:600	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	8	43	theme	microfibres	1553:1563	arg1	accumulation					1537:1548	the accumulation	1533:1548	the accumulation of microfibres	1533:1563	The remoteness of the deep sea does not prevent the accumulation of microfibres, being available to become integrated into deep sea organisms.					
30395638	1	44	theme	research	207:214	arg1	interest					216:223	stimulated considerable research interest	183:223	stimulated considerable research interest	183:223	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	2	45	theme	synthetic	449:457	arg1	textiles					459:466	synthetic textiles	449:466	synthetic textiles	449:466	However, relatively little attention has been paid to the fate and effects of microplastic particles that are fibrous in shape, also referred as microfibres, which are mostly shed from synthetic textiles during production or washing.					
30395638	5	46	theme	microfibres	1102:1112	arg1	microfibres					1102:1112	the sea floor microfibres	1088:1112	the sea floor microfibres	1088:1112	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	5	46	theme	microfibres	1102:1112	arg1	%					1083:1083	33%	1081:1083	33% of the sea floor microfibres	1081:1112	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	6	47	theme	downslope	1269:1277	arg1	transport					1279:1287	downslope transport	1269:1287	downslope transport of microfibres	1269:1302	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	5	48	theme	coastal	1054:1060	arg1	seas					1062:1065	coastal seas	1054:1065	coastal seas	1054:1065	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	4	49	dep	cellulose	830:838	arg1	polyethylene					895:906	polyethylene	895:906	polyethylene	895:906	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	4	49	dep	cellulose	830:838	arg1	polypropylene					913:925	polypropylene	913:925	polypropylene	913:925	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	4	49	dep	cellulose	830:838	arg1	polyamide					884:892	polyamide	884:892	polyamide	884:892	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	4	49	dep	cellulose	830:838	arg1	acrylic					875:881	acrylic	875:881	acrylic	875:881	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	4	49	dep	cellulose	830:838	arg1	polyester					864:872	polyester	864:872	polyester	864:872	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	0	50	theme	deep	48:51	arg1	seas					53:56	southern European deep seas	30:56	southern European deep seas	30:56	The imprint of microfibres in southern European deep seas.					
30395638	7	51	theme	deep	1442:1445	arg1	sea					1452:1454	the deep open sea	1438:1454	the deep open sea beyond 2000m of water depth	1438:1482	Around 20% of the microfibres found had accumulated in the deep open sea beyond 2000m of water depth.					
30395638	3	52	theme	limited	623:629	arg1	understanding					631:643	the limited understanding	619:643	the limited understanding of the long-range transport and magnitude of this type of microplastic pollution	619:724	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	5	53	theme	relative	1004:1011	arg1	abundance					1013:1021	the relative abundance	1000:1021	the relative abundance of fibres	1000:1031	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	2	54	from	fibrous	374:380	arg1	shape					385:389	shape	385:389	shape	385:389	However, relatively little attention has been paid to the fate and effects of microplastic particles that are fibrous in shape, also referred as microfibres, which are mostly shed from synthetic textiles during production or washing.					
30395638	1	55	theme	large	98:102	arg1	fragments					128:136	large and microscopic plastic fragments	98:136	large and microscopic plastic fragments	98:136	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	0	56	theme	European	39:46	arg1	seas					53:56	southern European deep seas	30:56	southern European deep seas	30:56	The imprint of microfibres in southern European deep seas.					
30395638	6	57	theme	microfibres	1292:1302	arg1	transport					1279:1287	downslope transport	1269:1287	downslope transport of microfibres	1269:1302	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	3	58	theme	transport	663:671	arg1	understanding					631:643	the limited understanding	619:643	the limited understanding of the long-range transport and magnitude of this type of microplastic pollution	619:724	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	3	59	theme	southern	579:586	arg1	seas					597:600	southern European seas	579:600	southern European seas	579:600	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	5	60	theme	greater	1119:1125	arg1	quantities					1127:1136	greater quantities	1119:1136	greater quantities of the fibres	1119:1150	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	6	61	theme	preferential	1243:1254	arg1	conduits					1256:1263	preferential conduits	1243:1263	preferential conduits for downslope transport of microfibres	1243:1302	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	6	61	theme	preferential	1243:1254	arg1	canyons					1228:1234	Submarine canyons	1218:1234	Submarine canyons	1218:1234	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	5	62	theme	fibres	1026:1031	arg1	abundance					1013:1021	the relative abundance	1000:1021	the relative abundance of fibres	1000:1031	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	5	63	theme	continuum	971:979	arg1	approach					981:988	a shelf-slope-deep basin continuum approach	946:988	a shelf-slope-deep basin continuum approach	946:988	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	2	64	from	shape	385:389	arg1	fibrous					374:380	fibrous	374:380	fibrous	374:380	However, relatively little attention has been paid to the fate and effects of microplastic particles that are fibrous in shape, also referred as microfibres, which are mostly shed from synthetic textiles during production or washing.					
30395638	1	65	theme	microscopic	108:118	arg1	fragments					128:136	large and microscopic plastic fragments	98:136	large and microscopic plastic fragments	98:136	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	3	66	theme	microfibres	542:552	arg1	abundance					529:537	abundance	529:537	abundance	529:537	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	3	66	theme	microfibres	542:552	arg1	composition					513:523	composition	513:523	composition	513:523	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	5	67	theme	open	1172:1175	arg1	sea					1177:1179	the open sea	1168:1179	the open sea	1168:1179	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	1	68	theme	plastic	120:126	arg1	fragments					128:136	large and microscopic plastic fragments	98:136	large and microscopic plastic fragments	98:136	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	7	69	theme	depth	1478:1482	arg1	2000m					1463:1467	2000m	1463:1467	2000m of water depth	1463:1482	Around 20% of the microfibres found had accumulated in the deep open sea beyond 2000m of water depth.					
30395638	6	70	theme	microfibres	1330:1340	arg1	%					1312:1312	29%	1310:1312	29% of the seafloor microfibres	1310:1340	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	6	70	theme	microfibres	1330:1340	arg1	microfibres					1330:1340	the seafloor microfibres	1317:1340	the seafloor microfibres	1317:1340	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	3	71	theme	long-range	652:661	arg1	transport					663:671	long-range transport	652:671	long-range transport	652:671	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	3	72	from	sediments	566:574	arg1	seas					597:600	southern European seas	579:600	southern European seas	579:600	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	5	73	dep	appear	1042:1047	arg1	Following					936:944	Following	936:944	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres	936:1031	Following a shelf-slope-deep basin continuum approach, based on the relative abundance of fibres it would appear that coastal seas retain around 33% of the sea floor microfibres, but greater quantities of the fibres are exported to the open sea, where they accumulate in sediments.					
30395638	6	74	theme	seafloor	1321:1328	arg1	microfibres					1330:1340	the seafloor microfibres	1317:1340	the seafloor microfibres	1317:1340	Submarine canyons act as preferential conduits for downslope transport of microfibres, with 29% of the seafloor microfibres compared to 18% found on the open slope.					
30395638	4	75	theme	sediment	781:788	arg1	ml					775:776	50 ml	772:776	50 ml of sediment	772:788	We report abundances of 10-70 microfibres in 50 ml of sediment, including both natural and regenerated cellulose, and synthetic plastic (polyester, acrylic, polyamide, polyethylene, and polypropylene) fibres.					
30395638	1	76	from	impacts	158:164	arg1	organisms					169:177	organisms	169:177	organisms	169:177	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
30395638	3	77	from	composition	513:523	arg1	sediments					566:574	seafloor sediments	557:574	seafloor sediments in southern European seas	557:600	Here we assess composition and abundance of microfibres in seafloor sediments in southern European seas, filling gaps in the limited understanding of the long-range transport and magnitude of this type of microplastic pollution.					
30395638	1	78	theme	widespread	242:251	arg1	publicity					253:261	widespread publicity	242:261	widespread publicity	242:261	Pollution of the marine environment by large and microscopic plastic fragments and their potential impacts on organisms has stimulated considerable research interest and has received widespread publicity.					
31730992	0	0	theme	hydrogel	97:104	arg1	dressings					112:120	hydrogel wound dressings	97:120	hydrogel wound dressings	97:120	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous mats for hydrogel wound dressings.					
31730992	2	1	theme	pure	508:511	arg1	solvent					519:525	pure water solvent	508:525	pure water solvent	508:525	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	5	2	dep	it	1227:1228	arg1	potential					1236:1244	great potential	1230:1244	it great potential for wound dressings	1227:1264	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	1	3	theme	wound	184:188	arg1	healing					190:196	wound healing	184:196	wound healing	184:196	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	1	4	theme	high	271:274	arg1	absorptivity					276:287	high absorptivity	271:287	high absorptivity by green electrospinning and crosslinking techniques	271:340	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	3	5	theme	nanofibers	731:740	arg1	diameter					715:722	diameter	715:722	diameter	715:722	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	3	5	theme	nanofibers	731:740	arg1	morphology					700:709	morphology	700:709	morphology	700:709	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	5	6	dep	meanwhile	1137:1145	arg1	capable					1172:1178	capable	1172:1178	capable	1172:1178	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	5	6	dep	meanwhile	1137:1145	arg1	cytocompatible					1153:1166	cytocompatible	1153:1166	cytocompatible	1153:1166	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	4	7	theme	MaPVA	1017:1021	arg1	molecules					996:1004	the molecules	992:1004	the molecules of MHA and MaPVA	992:1021	The crystalline microstructure of the electrospun fibers was not well developed due to intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA.					
31730992	4	8	theme	intermolecular	940:953	arg1	interaction					972:982	intermolecular hydrogen bonding interaction	940:982	intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA	940:1021	The crystalline microstructure of the electrospun fibers was not well developed due to intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA.					
31730992	4	9	theme	electrospun	891:901	arg1	fibers					903:908	the electrospun fibers	887:908	the electrospun fibers	887:908	The crystalline microstructure of the electrospun fibers was not well developed due to intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA.					
31730992	5	10	theme	photocrosslinking	1028:1044	arg1	mats					1068:1071	The photocrosslinking MHA/MaPVA nanofibrous mats	1024:1071	The photocrosslinking MHA/MaPVA nanofibrous mats	1024:1071	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	0	11	theme	wound	106:110	arg1	dressings					112:120	hydrogel wound dressings	97:120	hydrogel wound dressings	97:120	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous mats for hydrogel wound dressings.					
31730992	3	12	theme	weight	776:781	arg1	ratios					783:788	MHA/MaPVA weight ratios	766:788	MHA/MaPVA weight ratios	766:788	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	5	13	theme	MHA/MaPVA	1046:1054	arg1	mats					1068:1071	The photocrosslinking MHA/MaPVA nanofibrous mats	1024:1071	The photocrosslinking MHA/MaPVA nanofibrous mats	1024:1071	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	1	14	theme	green	292:296	arg1	electrospinning					298:312	green electrospinning	292:312	green electrospinning	292:312	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	5	15	theme	nanofibrous	1056:1066	arg1	mats					1068:1071	The photocrosslinking MHA/MaPVA nanofibrous mats	1024:1071	The photocrosslinking MHA/MaPVA nanofibrous mats	1024:1071	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	2	16	theme	vinyl	434:438	arg1	poly					428:431	hyaluronate/methacrylated poly	402:431	a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers	389:482	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	2	16	theme	vinyl	434:438	arg1	alcohol					440:446	vinyl alcohol	434:446	vinyl alcohol	434:446	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	0	17	theme	alcohol	67:73	arg1	nanofibrous					76:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous mats for hydrogel wound dressings.					
31730992	4	18	theme	fibers	903:908	arg1	microstructure					869:882	The crystalline microstructure	853:882	The crystalline microstructure of the electrospun fibers	853:908	The crystalline microstructure of the electrospun fibers was not well developed due to intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA.					
31730992	2	19	theme	maleilated	391:400	arg1	nanofibers					473:482	a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers	389:482	a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers	389:482	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	0	20	theme	maleilated	18:27	arg1	nanofibrous					76:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous mats for hydrogel wound dressings.					
31730992	3	21	theme	MHA/MaPVA	766:774	arg1	ratios					783:788	MHA/MaPVA weight ratios	766:788	MHA/MaPVA weight ratios	766:788	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	2	22	theme	poly	428:431	arg1	nanofibers					473:482	a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers	389:482	a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers	389:482	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	0	23	theme	Photocrosslinking	0:16	arg1	nanofibrous					76:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous mats for hydrogel wound dressings.					
31730992	2	24	theme	hyaluronate/methacrylated	402:426	arg1	MaPVA					456:460	MaPVA	456:460	MaPVA	456:460	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	2	24	theme	hyaluronate/methacrylated	402:426	arg1	poly					428:431	hyaluronate/methacrylated poly	402:431	a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers	389:482	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	2	24	theme	hyaluronate/methacrylated	402:426	arg1	alcohol					440:446	vinyl alcohol	434:446	vinyl alcohol	434:446	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	2	24	theme	hyaluronate/methacrylated	402:426	arg1	MHA					450:452	MHA	450:452	MHA	450:452	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	0	25	theme	poly	55:58	arg1	nanofibrous					76:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous mats for hydrogel wound dressings.					
31730992	5	26	theme	wound	1250:1254	arg1	dressings					1256:1264	wound dressings	1250:1264	wound dressings	1250:1264	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	2	27	theme	nanofibers	584:593	arg1	network					595:601	crosslinking nanofibers network	571:601	crosslinking nanofibers network	571:601	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	5	28	theme	high	1114:1117	arg1	absorption					1125:1134	high water absorption	1114:1134	high water absorption	1114:1134	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	5	29	theme	fibrous	1091:1097	arg1	hydrogels					1099:1107	fibrous hydrogels	1091:1107	fibrous hydrogels with high water absorption	1091:1134	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	5	29	theme	fibrous	1091:1097	arg1	meanwhile					1137:1145	meanwhile	1137:1145	meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings	1137:1264	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	0	30	theme	hyaluronate/methacrylated	29:53	arg1	nanofibrous					76:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous mats for hydrogel wound dressings.					
31730992	1	31	theme	crosslinking	318:329	arg1	techniques					331:340	crosslinking techniques	318:340	crosslinking techniques	318:340	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	2	32	theme	crosslinking	571:582	arg1	network					595:601	crosslinking nanofibers network	571:601	crosslinking nanofibers network	571:601	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	1	33	theme	fast	223:226	arg1	pace					228:231	a fast pace	221:231	a fast pace	221:231	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	0	34	theme	vinyl	61:65	arg1	nanofibrous					76:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous	0:86	Photocrosslinking maleilated hyaluronate/methacrylated poly (vinyl alcohol) nanofibrous mats for hydrogel wound dressings.					
31730992	4	35	theme	MHA	1009:1011	arg1	molecules					996:1004	the molecules	992:1004	the molecules of MHA and MaPVA	992:1021	The crystalline microstructure of the electrospun fibers was not well developed due to intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA.					
31730992	3	36	theme	systems	643:649	arg1	Electrospinnability					604:622	Electrospinnability	604:622	Electrospinnability of MHA/MaPVA blend systems	604:649	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	5	37	with	hydrogels	1099:1107	arg1	absorption					1125:1134	high water absorption	1114:1134	high water absorption	1114:1134	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	3	38	theme	blend	836:840	arg1	solutions					842:850	the blend solutions	832:850	the blend solutions	832:850	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	4	39	theme	bonding	964:970	arg1	interaction					972:982	intermolecular hydrogen bonding interaction	940:982	intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA	940:1021	The crystalline microstructure of the electrospun fibers was not well developed due to intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA.					
31730992	3	40	theme	MHA/MaPVA	627:635	arg1	systems					643:649	MHA/MaPVA blend systems	627:649	MHA/MaPVA blend systems	627:649	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	2	41	theme	composite	463:471	arg1	nanofibers					473:482	a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers	389:482	a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers	389:482	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	5	42	theme	great	1230:1234	arg1	potential					1236:1244	great potential	1230:1244	it great potential for wound dressings	1227:1264	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	4	43	theme	hydrogen	955:962	arg1	interaction					972:982	intermolecular hydrogen bonding interaction	940:982	intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA	940:1021	The crystalline microstructure of the electrospun fibers was not well developed due to intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA.					
31730992	5	44	theme	water	1119:1123	arg1	absorption					1125:1134	high water absorption	1114:1134	high water absorption	1114:1134	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	3	45	theme	blend	637:641	arg1	systems					643:649	MHA/MaPVA blend systems	627:649	MHA/MaPVA blend systems	627:649	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	1	46	theme	great	353:357	arg1	challenge					359:367	a great challenge	351:367	a great challenge	351:367	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	1	47	theme	nanofibrous	151:161	arg1	benefit					173:179	nanofibrous scaffolds benefit	151:179	nanofibrous scaffolds benefit	151:179	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	1	48	theme	scaffolds	163:171	arg1	benefit					173:179	nanofibrous scaffolds benefit	151:179	nanofibrous scaffolds benefit	151:179	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	1	49	theme	nanofibrous	244:254	arg1	scaffolds					256:264	nanofibrous scaffolds	244:264	nanofibrous scaffolds	244:264	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	4	50	theme	crystalline	857:867	arg1	microstructure					869:882	The crystalline microstructure	853:882	The crystalline microstructure of the electrospun fibers	853:908	The crystalline microstructure of the electrospun fibers was not well developed due to intermolecular hydrogen bonding interaction between the molecules of MHA and MaPVA.					
31730992	1	51	theme	benefit	173:179	arg1	electrospinning					132:146	electrospinning	132:146	electrospinning of nanofibrous scaffolds benefit to wound healing	132:196	Although electrospinning of nanofibrous scaffolds benefit to wound healing have been developed at a fast pace, achieving nanofibrous scaffolds with high absorptivity by green electrospinning and crosslinking techniques is still a great challenge.					
31730992	3	52	theme	solutions	842:850	arg1	viscosity					802:810	viscosity	802:810	viscosity	802:810	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	3	52	theme	solutions	842:850	arg1	conductivity					816:827	conductivity	816:827	conductivity	816:827	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
31730992	2	53	theme	water	513:517	arg1	solvent					519:525	pure water solvent	508:525	pure water solvent	508:525	Here, we developed a maleilated hyaluronate/methacrylated poly (vinyl alcohol) (MHA) (MaPVA) composite nanofibers by electrospinning from pure water solvent and followed by photopolymerization to form crosslinking nanofibers network.					
31730992	5	54	theme	cell	1197:1200	arg1	attachment					1202:1211	the cell attachment	1193:1211	the cell attachment	1193:1211	The photocrosslinking MHA/MaPVA nanofibrous mats can swell to form fibrous hydrogels with high water absorption, meanwhile it is cytocompatible and capable of promoting the cell attachment, which render it great potential for wound dressings.					
31730992	3	55	dep	morphology	700:709	arg1	the					696:698	the	696:698	the	696:698	Electrospinnability of MHA/MaPVA blend systems were investigated and the results shows that the morphology and diameter of the nanofibers were mainly affected by MHA/MaPVA weight ratios by changing viscosity and conductivity of the blend solutions.					
30521913	0	0	theme	films	100:104	arg1	synthesis					29:37	synthesis	29:37	synthesis of polymethyl methacrylate grafted sodium alginate composite films	29:104	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	4	1	from	resistance	865:874	arg1	properties					741:750	mechanical properties	730:750	mechanical properties	730:750	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	2	theme	water	859:863	arg1	resistance					865:874	water resistance	859:874	water resistance of composite films	859:893	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	2	3	theme	chains	346:351	arg1	grafting					329:336	the grafting	325:336	the grafting of PMMA chains onto the alginate backbone	325:378	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	5	4	theme	transparent	946:956	arg1	films					958:962	the homogeneous transparent films	930:962	the homogeneous transparent films	930:962	Results showed the homogeneous transparent films could be obtained while G% was lower than 8.64%.					
30521913	2	5	theme	electron	471:478	arg1	microscopy					480:489	scanning electron microscopy	462:489	scanning electron microscopy	462:489	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	1	6	theme	methyl	175:180	arg1	MMA					196:198	MMA	196:198	MMA	196:198	The graft copolymerization of methyl methacrylate (MMA) onto alginate was carried out in aqueous media by initiators of ammonium persulphate under a nitrogen gas atmosphere.					
30521913	1	6	theme	methyl	175:180	arg1	methacrylate					182:193	methyl methacrylate	175:193	methyl methacrylate (MMA)	175:199	The graft copolymerization of methyl methacrylate (MMA) onto alginate was carried out in aqueous media by initiators of ammonium persulphate under a nitrogen gas atmosphere.					
30521913	5	7	theme	homogeneous	934:944	arg1	films					958:962	the homogeneous transparent films	930:962	the homogeneous transparent films	930:962	Results showed the homogeneous transparent films could be obtained while G% was lower than 8.64%.					
30521913	0	8	theme	high	111:114	arg1	strength					116:123	high strength	111:123	high strength for food packaging	111:142	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	4	9	theme	mechanical	730:739	arg1	properties					741:750	mechanical properties	730:750	mechanical properties	730:750	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	3	10	theme	thin	550:553	arg1	film					612:615	a thin and transparent polymethyl methacrylate grafted alginate film	548:615	a thin and transparent polymethyl methacrylate grafted alginate film with high strength	548:634	At last, a thin and transparent polymethyl methacrylate grafted alginate film with high strength was obtained by the casting method.					
30521913	2	11	theme	resonance	515:523	arg1	spectroscopy					525:536	1H nuclear magnetic resonance spectroscopy	495:536	1H nuclear magnetic resonance spectroscopy	495:536	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	4	12	from	opacity	847:853	arg1	properties					741:750	mechanical properties	730:750	mechanical properties	730:750	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	13	theme	ratio	693:697	arg1	color					840:844	color	840:844	color	840:844	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	13	theme	ratio	693:697	arg1	effect					676:681	The effect	672:681	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties	672:750	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	13	theme	ratio	693:697	arg1	opacity					847:853	opacity	847:853	opacity	847:853	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	13	theme	ratio	693:697	arg1	analyses					829:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	13	theme	ratio	693:697	arg1	resistance					865:874	water resistance	859:874	water resistance of composite films	859:893	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	13	theme	ratio	693:697	arg1	stability					761:769	thermal stability	753:769	thermal stability (thermogravimetric analysis/differential thermogravimetry analyses)	753:837	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	1	14	theme	ammonium	265:272	arg1	persulphate					274:284	ammonium persulphate	265:284	ammonium persulphate under a nitrogen gas atmosphere	265:316	The graft copolymerization of methyl methacrylate (MMA) onto alginate was carried out in aqueous media by initiators of ammonium persulphate under a nitrogen gas atmosphere.					
30521913	2	15	theme	magnetic	506:513	arg1	resonance					515:523	1H nuclear magnetic resonance	495:523	1H nuclear magnetic resonance spectroscopy	495:536	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	2	16	dep	transform	423:431	arg1	infrared					433:440	infrared	433:440	infrared	433:440	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	7	17	theme	thermal	1225:1231	arg1	stability					1233:1241	thermal stability	1225:1241	thermal stability	1225:1241	On the other hand, water resistance, thermal stability and crystallinity of the films were improved, however, the transparency was slightly decreased.					
30521913	1	18	theme	methacrylate	182:193	arg1	copolymerization					155:170	The graft copolymerization	145:170	The graft copolymerization of methyl methacrylate (MMA) onto alginate	145:213	The graft copolymerization of methyl methacrylate (MMA) onto alginate was carried out in aqueous media by initiators of ammonium persulphate under a nitrogen gas atmosphere.					
30521913	2	19	theme	X-ray	443:447	arg1	diffraction					449:459	X-ray diffraction	443:459	X-ray diffraction	443:459	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	4	20	from	color	840:844	arg1	properties					741:750	mechanical properties	730:750	mechanical properties	730:750	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	0	21	theme	polymethyl	42:51	arg1	films					100:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	5	22	theme	G	988:988	arg1	%					989:989	G%	988:989	G%	988:989	Results showed the homogeneous transparent films could be obtained while G% was lower than 8.64%.					
30521913	6	23	from	break	1137:1141	arg1	%					1145:1145	E%	1144:1145	E%	1144:1145	After being grafted by PMMA, the tensile strength of the films was distinctly improved by 205.3%, as well the elongation at break (E%) by 41.7% while the MMA/SA (g/g) is 0.3.					
30521913	6	23	from	break	1137:1141	arg1	elongation					1123:1132	the elongation	1119:1132	the elongation at break	1119:1141	After being grafted by PMMA, the tensile strength of the films was distinctly improved by 205.3%, as well the elongation at break (E%) by 41.7% while the MMA/SA (g/g) is 0.3.					
30521913	6	23	from	break	1137:1141	arg1	%					1108:1108	205.3%	1103:1108	205.3%	1103:1108	After being grafted by PMMA, the tensile strength of the films was distinctly improved by 205.3%, as well the elongation at break (E%) by 41.7% while the MMA/SA (g/g) is 0.3.					
30521913	6	24	theme	tensile	1046:1052	arg1	strength					1054:1061	the tensile strength	1042:1061	the tensile strength of the films	1042:1074	After being grafted by PMMA, the tensile strength of the films was distinctly improved by 205.3%, as well the elongation at break (E%) by 41.7% while the MMA/SA (g/g) is 0.3.					
30521913	7	25	theme	films	1268:1272	arg1	crystallinity					1247:1259	crystallinity	1247:1259	crystallinity	1247:1259	On the other hand, water resistance, thermal stability and crystallinity of the films were improved, however, the transparency was slightly decreased.					
30521913	7	25	theme	films	1268:1272	arg1	resistance					1213:1222	water resistance	1207:1222	water resistance	1207:1222	On the other hand, water resistance, thermal stability and crystallinity of the films were improved, however, the transparency was slightly decreased.					
30521913	7	25	theme	films	1268:1272	arg1	stability					1233:1241	thermal stability	1225:1241	thermal stability	1225:1241	On the other hand, water resistance, thermal stability and crystallinity of the films were improved, however, the transparency was slightly decreased.					
30521913	0	26	theme	composite	90:98	arg1	films					100:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	2	27	theme	1H	495:496	arg1	resonance					515:523	1H nuclear magnetic resonance	495:523	1H nuclear magnetic resonance spectroscopy	495:536	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	4	28	theme	G	723:723	arg1	%					724:724	G%	723:724	G%	723:724	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	28	theme	G	723:723	arg1	percentage					702:711	percentage	702:711	percentage of graft (G%)	702:725	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	29	theme	composite	879:887	arg1	films					889:893	composite films	879:893	composite films	879:893	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	0	30	theme	Ammonium	0:7	arg1	persulphate					9:19	Ammonium persulphate	0:19	Ammonium persulphate	0:19	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	1	31	theme	persulphate	274:284	arg1	initiators					251:260	initiators	251:260	initiators of ammonium persulphate under a nitrogen gas atmosphere	251:316	The graft copolymerization of methyl methacrylate (MMA) onto alginate was carried out in aqueous media by initiators of ammonium persulphate under a nitrogen gas atmosphere.					
30521913	3	32	theme	transparent	559:569	arg1	film					612:615	a thin and transparent polymethyl methacrylate grafted alginate film	548:615	a thin and transparent polymethyl methacrylate grafted alginate film with high strength	548:634	At last, a thin and transparent polymethyl methacrylate grafted alginate film with high strength was obtained by the casting method.					
30521913	2	33	theme	alginate	362:369	arg1	backbone					371:378	the alginate backbone	358:378	the alginate backbone	358:378	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	6	34	theme	E	1144:1144	arg1	%					1145:1145	E%	1144:1145	E%	1144:1145	After being grafted by PMMA, the tensile strength of the films was distinctly improved by 205.3%, as well the elongation at break (E%) by 41.7% while the MMA/SA (g/g) is 0.3.					
30521913	6	34	theme	E	1144:1144	arg1	%					1108:1108	205.3%	1103:1108	205.3%	1103:1108	After being grafted by PMMA, the tensile strength of the films was distinctly improved by 205.3%, as well the elongation at break (E%) by 41.7% while the MMA/SA (g/g) is 0.3.					
30521913	3	35	theme	methacrylate	582:593	arg1	film					612:615	a thin and transparent polymethyl methacrylate grafted alginate film	548:615	a thin and transparent polymethyl methacrylate grafted alginate film with high strength	548:634	At last, a thin and transparent polymethyl methacrylate grafted alginate film with high strength was obtained by the casting method.					
30521913	4	36	theme	thermogravimetry	812:827	arg1	analyses					829:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	36	theme	thermogravimetry	812:827	arg1	stability					761:769	thermal stability	753:769	thermal stability (thermogravimetric analysis/differential thermogravimetry analyses)	753:837	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	7	37	theme	other	1195:1199	arg1	hand					1201:1204	the other hand	1191:1204	the other hand	1191:1204	On the other hand, water resistance, thermal stability and crystallinity of the films were improved, however, the transparency was slightly decreased.					
30521913	2	38	theme	Fourier	415:421	arg1	study					406:410	the study	402:410	the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy	402:536	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	0	39	theme	food	129:132	arg1	packaging					134:142	food packaging	129:142	food packaging	129:142	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	2	40	theme	PMMA	341:344	arg1	chains					346:351	PMMA chains	341:351	PMMA chains	341:351	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	2	41	theme	scanning	462:469	arg1	microscopy					480:489	scanning electron microscopy	462:489	scanning electron microscopy	462:489	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	1	42	theme	nitrogen	294:301	arg1	atmosphere					307:316	a nitrogen gas atmosphere	292:316	a nitrogen gas atmosphere	292:316	The graft copolymerization of methyl methacrylate (MMA) onto alginate was carried out in aqueous media by initiators of ammonium persulphate under a nitrogen gas atmosphere.					
30521913	3	43	theme	grafted	595:601	arg1	film					612:615	a thin and transparent polymethyl methacrylate grafted alginate film	548:615	a thin and transparent polymethyl methacrylate grafted alginate film with high strength	548:634	At last, a thin and transparent polymethyl methacrylate grafted alginate film with high strength was obtained by the casting method.					
30521913	7	44	theme	water	1207:1211	arg1	resistance					1213:1222	water resistance	1207:1222	water resistance	1207:1222	On the other hand, water resistance, thermal stability and crystallinity of the films were improved, however, the transparency was slightly decreased.					
30521913	1	45	theme	gas	303:305	arg1	atmosphere					307:316	a nitrogen gas atmosphere	292:316	a nitrogen gas atmosphere	292:316	The graft copolymerization of methyl methacrylate (MMA) onto alginate was carried out in aqueous media by initiators of ammonium persulphate under a nitrogen gas atmosphere.					
30521913	3	46	theme	alginate	603:610	arg1	film					612:615	a thin and transparent polymethyl methacrylate grafted alginate film	548:615	a thin and transparent polymethyl methacrylate grafted alginate film with high strength	548:634	At last, a thin and transparent polymethyl methacrylate grafted alginate film with high strength was obtained by the casting method.					
30521913	2	47	theme	nuclear	498:504	arg1	resonance					515:523	1H nuclear magnetic resonance	495:523	1H nuclear magnetic resonance spectroscopy	495:536	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	0	48	theme	grafted	66:72	arg1	films					100:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	4	49	theme	films	889:893	arg1	color					840:844	color	840:844	color	840:844	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	49	theme	films	889:893	arg1	effect					676:681	The effect	672:681	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties	672:750	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	49	theme	films	889:893	arg1	opacity					847:853	opacity	847:853	opacity	847:853	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	49	theme	films	889:893	arg1	analyses					829:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	49	theme	films	889:893	arg1	resistance					865:874	water resistance	859:874	water resistance of composite films	859:893	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	49	theme	films	889:893	arg1	stability					761:769	thermal stability	753:769	thermal stability (thermogravimetric analysis/differential thermogravimetry analyses)	753:837	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	3	50	theme	polymethyl	571:580	arg1	film					612:615	a thin and transparent polymethyl methacrylate grafted alginate film	548:615	a thin and transparent polymethyl methacrylate grafted alginate film with high strength	548:634	At last, a thin and transparent polymethyl methacrylate grafted alginate film with high strength was obtained by the casting method.					
30521913	4	51	theme	percentage	702:711	arg1	color					840:844	color	840:844	color	840:844	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	51	theme	percentage	702:711	arg1	effect					676:681	The effect	672:681	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties	672:750	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	51	theme	percentage	702:711	arg1	opacity					847:853	opacity	847:853	opacity	847:853	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	51	theme	percentage	702:711	arg1	analyses					829:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	51	theme	percentage	702:711	arg1	resistance					865:874	water resistance	859:874	water resistance of composite films	859:893	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	51	theme	percentage	702:711	arg1	stability					761:769	thermal stability	753:769	thermal stability (thermogravimetric analysis/differential thermogravimetry analyses)	753:837	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	0	52	theme	methacrylate	53:64	arg1	films					100:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	3	53	with	film	612:615	arg1	strength					627:634	high strength	622:634	high strength	622:634	At last, a thin and transparent polymethyl methacrylate grafted alginate film with high strength was obtained by the casting method.					
30521913	2	54	dep	Fourier	415:421	arg1	transform					423:431	transform	423:431	transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy	423:536	Then, the grafting of PMMA chains onto the alginate backbone was confirmed through the study of Fourier transform infrared, X-ray diffraction, scanning electron microscopy and 1H nuclear magnetic resonance spectroscopy.					
30521913	3	55	theme	high	622:625	arg1	strength					627:634	high strength	622:634	high strength	622:634	At last, a thin and transparent polymethyl methacrylate grafted alginate film with high strength was obtained by the casting method.					
30521913	4	56	theme	analysis/differential	790:810	arg1	analyses					829:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	56	theme	analysis/differential	790:810	arg1	stability					761:769	thermal stability	753:769	thermal stability (thermogravimetric analysis/differential thermogravimetry analyses)	753:837	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	57	theme	graft	716:720	arg1	%					724:724	G%	723:724	G%	723:724	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	57	theme	graft	716:720	arg1	percentage					702:711	percentage	702:711	percentage of graft (G%)	702:725	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	57	theme	graft	716:720	arg1	ratio					693:697	MMA/SA ratio	686:697	MMA/SA ratio	686:697	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	58	theme	MMA/SA	686:691	arg1	ratio					693:697	MMA/SA ratio	686:697	MMA/SA ratio	686:697	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	3	59	theme	casting	656:662	arg1	method					664:669	the casting method	652:669	the casting method	652:669	At last, a thin and transparent polymethyl methacrylate grafted alginate film with high strength was obtained by the casting method.					
30521913	4	60	theme	thermal	753:759	arg1	analyses					829:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	60	theme	thermal	753:759	arg1	stability					761:769	thermal stability	753:769	thermal stability (thermogravimetric analysis/differential thermogravimetry analyses)	753:837	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	6	61	theme	films	1070:1074	arg1	strength					1054:1061	the tensile strength	1042:1061	the tensile strength of the films	1042:1074	After being grafted by PMMA, the tensile strength of the films was distinctly improved by 205.3%, as well the elongation at break (E%) by 41.7% while the MMA/SA (g/g) is 0.3.					
30521913	0	62	theme	alginate	81:88	arg1	films					100:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	4	63	from	effect	676:681	arg1	properties					741:750	mechanical properties	730:750	mechanical properties	730:750	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	64	theme	thermogravimetric	772:788	arg1	analyses					829:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	thermogravimetric analysis/differential thermogravimetry analyses	772:836	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	4	64	theme	thermogravimetric	772:788	arg1	stability					761:769	thermal stability	753:769	thermal stability (thermogravimetric analysis/differential thermogravimetry analyses)	753:837	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	1	65	theme	graft	149:153	arg1	copolymerization					155:170	The graft copolymerization	145:170	The graft copolymerization of methyl methacrylate (MMA) onto alginate	145:213	The graft copolymerization of methyl methacrylate (MMA) onto alginate was carried out in aqueous media by initiators of ammonium persulphate under a nitrogen gas atmosphere.					
30521913	1	66	theme	aqueous	234:240	arg1	media					242:246	aqueous media	234:246	aqueous media	234:246	The graft copolymerization of methyl methacrylate (MMA) onto alginate was carried out in aqueous media by initiators of ammonium persulphate under a nitrogen gas atmosphere.					
30521913	0	67	theme	sodium	74:79	arg1	films					100:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	polymethyl methacrylate grafted sodium alginate composite films	42:104	Ammonium persulphate induced synthesis of polymethyl methacrylate grafted sodium alginate composite films with high strength for food packaging.					
30521913	4	68	from	stability	761:769	arg1	properties					741:750	mechanical properties	730:750	mechanical properties	730:750	The effect of MMA/SA ratio or percentage of graft (G%) on mechanical properties, thermal stability (thermogravimetric analysis/differential thermogravimetry analyses), color, opacity and water resistance of composite films were characterized.					
30521913	5	69	dep	showed	923:928	arg1	obtained					973:980	obtained	973:980	showed the homogeneous transparent films could be obtained while G% was lower than 8.64%	923:1010	Results showed the homogeneous transparent films could be obtained while G% was lower than 8.64%.					
30501759	5	0	theme	CuNPs/GO-CS	817:827	arg1	nanocomposites					830:843	the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	767:843	the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	767:843	The characteristics of the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites was found to be superior to the GO-CS.					
30501759	6	1	theme	UV-visible	960:969	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	1	theme	UV-visible	960:969	arg1	spectroscopy					971:982	UV-visible spectroscopy	960:982	UV-visible spectroscopy	960:982	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	4	2	from	study	548:552	arg1	embedded					598:605	embedded	598:605	embedded	598:605	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	1	3	theme	nanoparticle	163:174	arg1	synthesis					176:184	nanoparticle synthesis	163:184	nanoparticle synthesis	163:184	In the current scenario, nanoparticle synthesis has been fascinated by biogenic approaches than the chemical methods.					
30501759	5	4	theme	nanoparticles/graphene	778:799	arg1	nanocomposites					830:843	the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	767:843	the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	767:843	The characteristics of the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites was found to be superior to the GO-CS.					
30501759	7	5	theme	MCF7	1364:1367	arg1	lines					1374:1378	MCF7 cell lines	1364:1378	MCF7 cell lines	1364:1378	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	6	6	theme	fluorescence	1051:1062	arg1	spectroscopy					1064:1075	fluorescence spectroscopy	1051:1075	fluorescence spectroscopy	1051:1075	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	6	theme	fluorescence	1051:1062	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	7	7	theme	anti-bacterial	1264:1277	arg1	activity					1279:1286	their anti-bacterial activity	1258:1286	their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines	1258:1378	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	7	8	theme	anti-cancer	1326:1336	arg1	activity					1338:1345	anti-cancer activity	1326:1345	anti-cancer activity on breast cancer	1326:1362	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	4	9	theme	oxide-chitosan	623:636	arg1	polymer					646:652	graphene oxide-chitosan (GO-CS) polymer	614:652	graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells	614:741	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	0	10	theme	Anti-Bacterial	97:110	arg1	Activities					126:135	Its Anti-Bacterial and Cytotoxic Activities	93:135	Its Anti-Bacterial and Cytotoxic Activities	93:135	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	6	11	theme	diffraction	937:947	arg1	microscope					1102:1111	confocal laser scanning microscope	1078:1111	confocal laser scanning microscope	1078:1111	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	11	theme	diffraction	937:947	arg1	spectroscopy					1064:1075	fluorescence spectroscopy	1051:1075	fluorescence spectroscopy	1051:1075	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	11	theme	diffraction	937:947	arg1	spectroscopy					971:982	UV-visible spectroscopy	960:982	UV-visible spectroscopy	960:982	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	11	theme	diffraction	937:947	arg1	fourier					1010:1016	fourier	1010:1016	fourier transform infrared spectroscopy	1010:1048	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	11	theme	diffraction	937:947	arg1	microscopy					1135:1144	scanning electron microscopy	1117:1144	scanning electron microscopy	1117:1144	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	11	theme	diffraction	937:947	arg1	microscope					998:1007	atomic force microscope	985:1007	atomic force microscope	985:1007	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	11	theme	diffraction	937:947	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	7	12	dep	Escherichia	1296:1306	arg1	coli					1308:1311	coli	1308:1311	coli	1308:1311	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	4	13	theme	cancer	730:735	arg1	cells					737:741	cancer cells	730:741	cancer cells	730:741	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	4	14	theme	graphene	614:621	arg1	polymer					646:652	graphene oxide-chitosan (GO-CS) polymer	614:652	graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells	614:741	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	3	15	theme	more	377:380	arg1	advantages					382:391	more advantages	377:391	more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing	377:537	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	3	15	theme	more	377:380	arg1	potential					405:413	the potential	401:413	the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing	401:537	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	6	16	theme	X-ray	931:935	arg1	microscope					1102:1111	confocal laser scanning microscope	1078:1111	confocal laser scanning microscope	1078:1111	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	16	theme	X-ray	931:935	arg1	spectroscopy					1064:1075	fluorescence spectroscopy	1051:1075	fluorescence spectroscopy	1051:1075	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	16	theme	X-ray	931:935	arg1	spectroscopy					971:982	UV-visible spectroscopy	960:982	UV-visible spectroscopy	960:982	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	16	theme	X-ray	931:935	arg1	fourier					1010:1016	fourier	1010:1016	fourier transform infrared spectroscopy	1010:1048	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	16	theme	X-ray	931:935	arg1	microscopy					1135:1144	scanning electron microscopy	1117:1144	scanning electron microscopy	1117:1144	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	16	theme	X-ray	931:935	arg1	microscope					998:1007	atomic force microscope	985:1007	atomic force microscope	985:1007	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	16	theme	X-ray	931:935	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	7	17	theme	nanoparticles/graphene	1174:1195	arg1	CuNPs/GO-CS					1213:1223	CuNPs/GO-CS	1213:1223	CuNPs/GO-CS	1213:1223	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	7	17	theme	nanoparticles/graphene	1174:1195	arg1	oxide-chitosan					1197:1210	new copper nanoparticles/graphene oxide-chitosan	1163:1210	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	1147:1239	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	6	18	theme	scanning	1093:1100	arg1	microscope					1102:1111	confocal laser scanning microscope	1078:1111	confocal laser scanning microscope	1078:1111	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	18	theme	scanning	1093:1100	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	4	19	from	effects	703:709	arg1	normal					719:724	normal	719:724	normal	719:724	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	4	19	from	effects	703:709	arg1	cells					737:741	cancer cells	730:741	cancer cells	730:741	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	3	20	theme	easier	474:479	arg1	immunity					436:443	immunity	436:443	immunity towards toxicity	436:460	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	3	20	theme	easier	474:479	arg1	bioaccumulation					419:433	bioaccumulation	419:433	bioaccumulation	419:433	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	0	21	theme	Cytotoxic	116:124	arg1	Activities					126:135	Its Anti-Bacterial and Cytotoxic Activities	93:135	Its Anti-Bacterial and Cytotoxic Activities	93:135	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	0	22	from	Embedded	43:50	arg1	Composite					79:87	Graphene Oxide-Chitosan Composite	55:87	Graphene Oxide-Chitosan Composite	55:87	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	0	22	from	Embedded	43:50	arg1	Activities					126:135	Its Anti-Bacterial and Cytotoxic Activities	93:135	Its Anti-Bacterial and Cytotoxic Activities	93:135	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	7	23	theme	oxide-chitosan	1197:1210	arg1	nanocomposites					1226:1239	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	1147:1239	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	1147:1239	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	3	24	from	synthesis	503:511	arg1	simpler					492:498	simpler	492:498	simpler	492:498	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	2	25	theme	source	288:293	arg1	reproducibility					265:279	reproducibility	265:279	reproducibility of the source	265:293	However, reproducibility of the source stands essentially in nanoparticle synthesis.					
30501759	5	26	theme	copper	771:776	arg1	nanocomposites					830:843	the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	767:843	the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	767:843	The characteristics of the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites was found to be superior to the GO-CS.					
30501759	7	27	theme	new	1163:1165	arg1	CuNPs/GO-CS					1213:1223	CuNPs/GO-CS	1213:1223	CuNPs/GO-CS	1213:1223	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	7	27	theme	new	1163:1165	arg1	oxide-chitosan					1197:1210	new copper nanoparticles/graphene oxide-chitosan	1163:1210	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	1147:1239	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	7	28	theme	Escherichia	1296:1306	arg1	443					1318:1320	Escherichia coli MTCC 443	1296:1320	Escherichia coli MTCC 443	1296:1320	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	6	29	theme	infrared	1028:1035	arg1	spectroscopy					1037:1048	infrared spectroscopy	1028:1048	infrared spectroscopy	1028:1048	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	7	30	theme	copper	1167:1172	arg1	CuNPs/GO-CS					1213:1223	CuNPs/GO-CS	1213:1223	CuNPs/GO-CS	1213:1223	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	7	30	theme	copper	1167:1172	arg1	oxide-chitosan					1197:1210	new copper nanoparticles/graphene oxide-chitosan	1163:1210	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	1147:1239	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	6	31	dep	fourier	1010:1016	arg1	transform					1018:1026	transform	1018:1026	transform infrared spectroscopy	1018:1048	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	0	32	theme	Copper	22:27	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Biogenic Copper	0:27	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	3	33	from	processing	528:537	arg1	simpler					492:498	simpler	492:498	simpler	492:498	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	1	34	theme	biogenic	209:216	arg1	approaches					218:227	biogenic approaches	209:227	biogenic approaches than the chemical methods	209:253	In the current scenario, nanoparticle synthesis has been fascinated by biogenic approaches than the chemical methods.					
30501759	0	35	theme	Biogenic	13:20	arg1	Copper					22:27	Biogenic Copper	13:27	Biogenic Copper	13:27	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	7	36	theme	synthesized	1151:1161	arg1	nanocomposites					1226:1239	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	1147:1239	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	1147:1239	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	2	37	theme	nanoparticle	317:328	arg1	synthesis					330:338	nanoparticle synthesis	317:338	nanoparticle synthesis	317:338	However, reproducibility of the source stands essentially in nanoparticle synthesis.					
30501759	6	38	theme	scanning	1117:1124	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	38	theme	scanning	1117:1124	arg1	microscopy					1135:1144	scanning electron microscopy	1117:1144	scanning electron microscopy	1117:1144	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	5	39	theme	nanocomposites	830:843	arg1	superior					861:868	superior	861:868	superior	861:868	The characteristics of the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites was found to be superior to the GO-CS.					
30501759	5	39	theme	nanocomposites	830:843	arg1	characteristics					748:762	The characteristics	744:762	The characteristics of the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	744:843	The characteristics of the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites was found to be superior to the GO-CS.					
30501759	7	40	theme	MTCC	1313:1316	arg1	443					1318:1320	Escherichia coli MTCC 443	1296:1320	Escherichia coli MTCC 443	1296:1320	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	4	41	theme	biocompatibility	668:683	arg1	effects					703:709	the biocompatibility and toxicological effects	664:709	the biocompatibility and toxicological effects on both normal and cancer cells	664:741	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	4	42	theme	toxicological	689:701	arg1	effects					703:709	the biocompatibility and toxicological effects	664:709	the biocompatibility and toxicological effects on both normal and cancer cells	664:741	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	4	43	theme	biogenic	555:562	arg1	CuNPs					586:590	CuNPs	586:590	CuNPs	586:590	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	4	43	theme	biogenic	555:562	arg1	nanoparticles					571:583	biogenic copper nanoparticles	555:583	biogenic copper nanoparticles (CuNPs)	555:591	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	0	44	from	Composite	79:87	arg1	Embedded					43:50	Embedded	43:50	Embedded	43:50	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	6	45	from	results	909:915	arg1	evident					892:898	evident	892:898	evident	892:898	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	4	46	from	embedded	598:605	arg1	study					548:552	this study	543:552	this study	543:552	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	0	47	theme	Graphene	55:62	arg1	Composite					79:87	Graphene Oxide-Chitosan Composite	55:87	Graphene Oxide-Chitosan Composite	55:87	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	6	48	theme	force	992:996	arg1	microscope					998:1007	atomic force microscope	985:1007	atomic force microscope	985:1007	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	48	theme	force	992:996	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	7	49	theme	breast	1350:1355	arg1	cancer					1357:1362	breast cancer	1350:1362	breast cancer	1350:1362	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	3	50	dep	easier	474:479	arg1	simpler					492:498	simpler	492:498	simpler	492:498	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	3	51	theme	downstream	517:526	arg1	processing					528:537	downstream processing	517:537	downstream processing	517:537	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	6	52	theme	atomic	985:990	arg1	microscope					998:1007	atomic force microscope	985:1007	atomic force microscope	985:1007	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	52	theme	atomic	985:990	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	3	53	theme	Nanoparticle	341:352	arg1	synthesis					354:362	Nanoparticle synthesis	341:362	Nanoparticle synthesis by fungi	341:371	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	0	54	from	Activities	126:135	arg1	Embedded					43:50	Embedded	43:50	Embedded	43:50	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	3	55	from	simpler	492:498	arg1	synthesis					503:511	synthesis	503:511	synthesis	503:511	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	3	55	from	simpler	492:498	arg1	processing					528:537	downstream processing	517:537	downstream processing	517:537	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	7	56	from	443	1318:1320	arg1	cancer					1357:1362	breast cancer	1350:1362	breast cancer	1350:1362	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	4	57	theme	copper	564:569	arg1	CuNPs					586:590	CuNPs	586:590	CuNPs	586:590	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	4	57	theme	copper	564:569	arg1	nanoparticles					571:583	biogenic copper nanoparticles	555:583	biogenic copper nanoparticles (CuNPs)	555:591	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
30501759	7	58	dep	443	1318:1320	arg1	lines					1374:1378	MCF7 cell lines	1364:1378	MCF7 cell lines	1364:1378	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	7	59	from	activity	1338:1345	arg1	cancer					1357:1362	breast cancer	1350:1362	breast cancer	1350:1362	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	1	60	theme	chemical	238:245	arg1	methods					247:253	the chemical methods	234:253	the chemical methods	234:253	In the current scenario, nanoparticle synthesis has been fascinated by biogenic approaches than the chemical methods.					
30501759	1	61	theme	current	145:151	arg1	scenario					153:160	the current scenario	141:160	the current scenario	141:160	In the current scenario, nanoparticle synthesis has been fascinated by biogenic approaches than the chemical methods.					
30501759	6	62	theme	laser	1087:1091	arg1	microscope					1102:1111	confocal laser scanning microscope	1078:1111	confocal laser scanning microscope	1078:1111	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	62	theme	laser	1087:1091	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	5	63	theme	oxide-chitosan	801:814	arg1	nanocomposites					830:843	the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	767:843	the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites	767:843	The characteristics of the copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites was found to be superior to the GO-CS.					
30501759	6	64	theme	electron	1126:1133	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	64	theme	electron	1126:1133	arg1	microscopy					1135:1144	scanning electron microscopy	1117:1144	scanning electron microscopy	1117:1144	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	7	65	theme	cell	1369:1372	arg1	lines					1374:1378	MCF7 cell lines	1364:1378	MCF7 cell lines	1364:1378	The synthesized new copper nanoparticles/graphene oxide-chitosan (CuNPs/GO-CS) nanocomposites were studied for their anti-bacterial activity against Escherichia coli MTCC 443 and anti-cancer activity on breast cancer MCF7 cell lines.					
30501759	6	66	theme	confocal	1078:1085	arg1	microscope					1102:1111	confocal laser scanning microscope	1078:1111	confocal laser scanning microscope	1078:1111	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	6	66	theme	confocal	1078:1085	arg1	technique					949:957	X-ray diffraction technique	931:957	X-ray diffraction technique	931:957	This is evident from the results obtained from X-ray diffraction technique, UV-visible spectroscopy, atomic force microscope, fourier transform infrared spectroscopy, fluorescence spectroscopy, confocal laser scanning microscope and scanning electron microscopy.					
30501759	0	67	theme	Oxide-Chitosan	64:77	arg1	Composite					79:87	Graphene Oxide-Chitosan Composite	55:87	Graphene Oxide-Chitosan Composite	55:87	Synthesis of Biogenic Copper Nanoparticles Embedded in Graphene Oxide-Chitosan Composite and Its Anti-Bacterial and Cytotoxic Activities.					
30501759	3	68	contain	has	373:375	arg1	synthesis					354:362	Nanoparticle synthesis	341:362	Nanoparticle synthesis by fungi	341:371	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	3	68	contain	has	373:375	arg2	advantages					382:391	more advantages	377:391	more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing	377:537	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	3	68	contain	has	373:375	arg2	potential					405:413	the potential	401:413	the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing	401:537	Nanoparticle synthesis by fungi has more advantages such as the potential for bioaccumulation, immunity towards toxicity, relatively easier to handle, simpler in synthesis and downstream processing.					
30501759	4	69	theme	GO-CS	639:643	arg1	polymer					646:652	graphene oxide-chitosan (GO-CS) polymer	614:652	graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells	614:741	In this study, biogenic copper nanoparticles (CuNPs) were embedded within graphene oxide-chitosan (GO-CS) polymer to endure the biocompatibility and toxicological effects on both normal and cancer cells.					
29627469	0	0	theme	dye	99:101	arg1	adsorption					103:112	dye adsorption	99:112	dye adsorption	99:112	Magnetic N-doped carbon aerogel from sodium carboxymethyl cellulose/collagen composite aerogel for dye adsorption and electrochemical supercapacitor.					
29627469	2	1	theme	excellent	560:568	arg1	properties					579:588	excellent magnetic properties	560:588	excellent magnetic properties	560:588	The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties.					
29627469	6	2	used	utilized	1067:1074	arg2	aerogels					1051:1058	N-doped carbon aerogels	1036:1058	N-doped carbon aerogels	1036:1058	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	6	2	used	utilized	1067:1074	arg2	electrodes					1079:1088	electrodes	1079:1088	electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions	1079:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	3	3	theme	current	688:694	arg1	density					696:702	the current density	684:702	the current density of 0.5A/g in a 6M KOH electrolyte	684:736	When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte.					
29627469	1	4	theme	sodium	194:199	arg1	cellulose					215:223	sodium carboxymethyl cellulose	194:223	sodium carboxymethyl cellulose	194:223	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	5	theme	cross-linking	388:400	arg1	agent					402:406	cross-linking agent	388:406	cross-linking agent	388:406	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	5	theme	cross-linking	388:400	arg1	collagen					366:373	collagen	366:373	collagen	366:373	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	5	theme	cross-linking	388:400	arg1	trichloride					350:360	ferric trichloride	343:360	ferric trichloride	343:360	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	6	6	from	supercapacitors	1093:1107	arg1	solutions					1171:1179	aqueous solutions	1163:1179	aqueous solutions	1163:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	1	7	theme	carboxymethyl	201:213	arg1	cellulose					215:223	sodium carboxymethyl cellulose	194:223	sodium carboxymethyl cellulose	194:223	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	4	8	theme	cycling	841:847	arg1	stability					849:857	excellent cycling stability	831:857	excellent cycling stability	831:857	The specific capacitance retention was 90.2% after 5000 charge/discharge cycles, indicating excellent cycling stability.					
29627469	4	9	theme	excellent	831:839	arg1	stability					849:857	excellent cycling stability	831:857	excellent cycling stability	831:857	The specific capacitance retention was 90.2% after 5000 charge/discharge cycles, indicating excellent cycling stability.					
29627469	1	10	theme	KOH	317:319	arg1	processes					332:340	KOH activation processes	317:340	KOH activation processes	317:340	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	11	attach	derived	181:187	arg1	cellulose					215:223	sodium carboxymethyl cellulose	194:223	sodium carboxymethyl cellulose	194:223	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	11	attach	derived	181:187	arg2	aerogels					172:179	Nitrogen-doped carbon aerogels	150:179	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose	150:223	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	2	12	theme	well-developed	473:486	arg1	morphologies					513:524	well-developed porous three-dimensional morphologies	473:524	well-developed porous three-dimensional morphologies	473:524	The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties.					
29627469	3	13	theme	electrode	607:615	arg1	material					617:624	electrode material	607:624	electrode material	607:624	When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte.					
29627469	6	14	theme	aqueous	1163:1169	arg1	solutions					1171:1179	aqueous solutions	1163:1179	aqueous solutions	1163:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	2	15	theme	carbon	447:452	arg1	aerogels					454:461	The carbon aerogels	443:461	The carbon aerogels	443:461	The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties.					
29627469	0	16	theme	electrochemical	118:132	arg1	supercapacitor					134:147	electrochemical supercapacitor	118:147	electrochemical supercapacitor	118:147	Magnetic N-doped carbon aerogel from sodium carboxymethyl cellulose/collagen composite aerogel for dye adsorption and electrochemical supercapacitor.					
29627469	5	17	theme	carbon	877:882	arg1	aerogels					884:891	the carbon aerogels	873:891	the carbon aerogels	873:891	In addition, the carbon aerogels showed excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue, respectively.					
29627469	3	18	from	density	696:702	arg1	electrolyte					726:736	a 6M KOH electrolyte	717:736	a 6M KOH electrolyte	717:736	When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte.					
29627469	3	18	from	density	696:702	arg1	capacitance					657:667	a specific capacitance	646:667	a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte	646:736	When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte.					
29627469	5	19	theme	230.4mg/g	945:953	arg1	capacities					921:930	excellent adsorption capacities	900:930	excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue	900:992	In addition, the carbon aerogels showed excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue, respectively.					
29627469	0	20	theme	N-doped	9:15	arg1	aerogel					24:30	Magnetic N-doped carbon aerogel	0:30	Magnetic N-doped carbon aerogel from sodium carboxymethyl	0:56	Magnetic N-doped carbon aerogel from sodium carboxymethyl cellulose/collagen composite aerogel for dye adsorption and electrochemical supercapacitor.					
29627469	6	21	theme	carbon	1044:1049	arg1	electrodes					1079:1088	electrodes	1079:1088	electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions	1079:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	6	21	theme	carbon	1044:1049	arg1	aerogels					1051:1058	N-doped carbon aerogels	1036:1058	N-doped carbon aerogels	1036:1058	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	1	22	theme	nitrogen	412:419	arg1	collagen					366:373	collagen	366:373	collagen	366:373	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	22	theme	nitrogen	412:419	arg1	source					421:426	nitrogen source	412:426	nitrogen source	412:426	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	22	theme	nitrogen	412:419	arg1	trichloride					350:360	ferric trichloride	343:360	ferric trichloride	343:360	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	0	23	from	carboxymethyl	44:56	arg1	aerogel					24:30	Magnetic N-doped carbon aerogel	0:30	Magnetic N-doped carbon aerogel from sodium carboxymethyl	0:56	Magnetic N-doped carbon aerogel from sodium carboxymethyl cellulose/collagen composite aerogel for dye adsorption and electrochemical supercapacitor.					
29627469	0	24	theme	Magnetic	0:7	arg1	aerogel					24:30	Magnetic N-doped carbon aerogel	0:30	Magnetic N-doped carbon aerogel from sodium carboxymethyl	0:56	Magnetic N-doped carbon aerogel from sodium carboxymethyl cellulose/collagen composite aerogel for dye adsorption and electrochemical supercapacitor.					
29627469	1	25	theme	activation	321:330	arg1	processes					332:340	KOH activation processes	317:340	KOH activation processes	317:340	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	6	26	from	electrodes	1079:1088	arg1	adsorbents					1149:1158	highly efficient contaminant adsorbents	1120:1158	supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions	1093:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	6	26	from	electrodes	1079:1088	arg1	supercapacitors					1093:1107	supercapacitors	1093:1107	supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions	1093:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	6	27	from	adsorbents	1149:1158	arg1	solutions					1171:1179	aqueous solutions	1163:1179	aqueous solutions	1163:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	3	28	theme	specific	648:655	arg1	capacitance					657:667	a specific capacitance	646:667	a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte	646:736	When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte.					
29627469	5	29	theme	malachite	959:967	arg1	green					969:973	malachite green	959:973	malachite green	959:973	In addition, the carbon aerogels showed excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue, respectively.					
29627469	4	30	theme	charge/discharge	795:810	arg1	cycles					812:817	5000 charge/discharge cycles	790:817	5000 charge/discharge cycles	790:817	The specific capacitance retention was 90.2% after 5000 charge/discharge cycles, indicating excellent cycling stability.					
29627469	2	31	theme	porous	488:493	arg1	morphologies					513:524	well-developed porous three-dimensional morphologies	473:524	well-developed porous three-dimensional morphologies	473:524	The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties.					
29627469	0	32	theme	carbon	17:22	arg1	aerogel					24:30	Magnetic N-doped carbon aerogel	0:30	Magnetic N-doped carbon aerogel from sodium carboxymethyl	0:56	Magnetic N-doped carbon aerogel from sodium carboxymethyl cellulose/collagen composite aerogel for dye adsorption and electrochemical supercapacitor.					
29627469	5	33	theme	blue	989:992	arg1	methylene					979:987	methylene blue	979:992	methylene blue	979:992	In addition, the carbon aerogels showed excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue, respectively.					
29627469	1	34	theme	multistep	245:253	arg1	approach					255:262	a multistep approach	243:262	a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes	243:340	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	0	35	theme	sodium	37:42	arg1	carboxymethyl					44:56	sodium carboxymethyl	37:56	sodium carboxymethyl	37:56	Magnetic N-doped carbon aerogel from sodium carboxymethyl cellulose/collagen composite aerogel for dye adsorption and electrochemical supercapacitor.					
29627469	1	36	theme	ferric	343:348	arg1	collagen					366:373	collagen	366:373	collagen	366:373	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	36	theme	ferric	343:348	arg1	agent					402:406	cross-linking agent	388:406	cross-linking agent	388:406	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	36	theme	ferric	343:348	arg1	source					421:426	nitrogen source	412:426	nitrogen source	412:426	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	36	theme	ferric	343:348	arg1	trichloride					350:360	ferric trichloride	343:360	ferric trichloride	343:360	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	3	37	theme	6M	719:720	arg1	electrolyte					726:736	a 6M KOH electrolyte	717:736	a 6M KOH electrolyte	717:736	When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte.					
29627469	3	38	theme	185.3F/g	672:679	arg1	capacitance					657:667	a specific capacitance	646:667	a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte	646:736	When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte.					
29627469	6	39	theme	efficient	1127:1135	arg1	adsorbents					1149:1158	highly efficient contaminant adsorbents	1120:1158	supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions	1093:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	6	40	theme	N-doped	1036:1042	arg1	electrodes					1079:1088	electrodes	1079:1088	electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions	1079:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	6	40	theme	N-doped	1036:1042	arg1	aerogels					1051:1058	N-doped carbon aerogels	1036:1058	N-doped carbon aerogels	1036:1058	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	3	41	theme	0.5A/g	707:712	arg1	density					696:702	the current density	684:702	the current density of 0.5A/g in a 6M KOH electrolyte	684:736	When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte.					
29627469	6	42	theme	contaminant	1137:1147	arg1	adsorbents					1149:1158	highly efficient contaminant adsorbents	1120:1158	supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions	1093:1179	These results suggest that N-doped carbon aerogels may be utilized as electrodes in supercapacitors as well as highly efficient contaminant adsorbents in aqueous solutions.					
29627469	5	43	theme	238.2	935:939	arg1	capacities					921:930	excellent adsorption capacities	900:930	excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue	900:992	In addition, the carbon aerogels showed excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue, respectively.					
29627469	2	44	theme	surface	541:547	arg1	areas					549:553	high specific surface areas	527:553	high specific surface areas	527:553	The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties.					
29627469	2	45	theme	magnetic	570:577	arg1	properties					579:588	excellent magnetic properties	560:588	excellent magnetic properties	560:588	The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties.					
29627469	2	46	theme	specific	532:539	arg1	areas					549:553	high specific surface areas	527:553	high specific surface areas	527:553	The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties.					
29627469	1	47	theme	Nitrogen-doped	150:163	arg1	aerogels					172:179	Nitrogen-doped carbon aerogels	150:179	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose	150:223	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	48	used	used	380:383	arg2	collagen					366:373	collagen	366:373	collagen	366:373	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	48	used	used	380:383	arg2	agent					402:406	cross-linking agent	388:406	cross-linking agent	388:406	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	48	used	used	380:383	arg2	trichloride					350:360	ferric trichloride	343:360	ferric trichloride	343:360	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	48	used	used	380:383	arg2	source					421:426	nitrogen source	412:426	nitrogen source	412:426	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	1	49	theme	carbon	165:170	arg1	aerogels					172:179	Nitrogen-doped carbon aerogels	150:179	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose	150:223	Nitrogen-doped carbon aerogels derived from sodium carboxymethyl cellulose were prepared via a multistep approach involving sol-gel, freeze-drying, carbonization, and KOH activation processes; ferric trichloride and collagen were used as cross-linking agent and nitrogen source, respectively.					
29627469	3	50	theme	KOH	722:724	arg1	electrolyte					726:736	a 6M KOH electrolyte	717:736	a 6M KOH electrolyte	717:736	When applied as electrode material, the CA-N0.5 showed a specific capacitance of 185.3F/g at the current density of 0.5A/g in a 6M KOH electrolyte.					
29627469	5	51	theme	excellent	900:908	arg1	capacities					921:930	excellent adsorption capacities	900:930	excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue	900:992	In addition, the carbon aerogels showed excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue, respectively.					
29627469	4	52	theme	specific	743:750	arg1	retention					764:772	The specific capacitance retention	739:772	The specific capacitance retention	739:772	The specific capacitance retention was 90.2% after 5000 charge/discharge cycles, indicating excellent cycling stability.					
29627469	4	52	theme	specific	743:750	arg1	%					782:782	90.2%	778:782	90.2%	778:782	The specific capacitance retention was 90.2% after 5000 charge/discharge cycles, indicating excellent cycling stability.					
29627469	5	53	theme	adsorption	910:919	arg1	capacities					921:930	excellent adsorption capacities	900:930	excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue	900:992	In addition, the carbon aerogels showed excellent adsorption capacities of 238.2 and 230.4mg/g for malachite green and methylene blue, respectively.					
29627469	4	54	theme	capacitance	752:762	arg1	retention					764:772	The specific capacitance retention	739:772	The specific capacitance retention	739:772	The specific capacitance retention was 90.2% after 5000 charge/discharge cycles, indicating excellent cycling stability.					
29627469	4	54	theme	capacitance	752:762	arg1	%					782:782	90.2%	778:782	90.2%	778:782	The specific capacitance retention was 90.2% after 5000 charge/discharge cycles, indicating excellent cycling stability.					
29627469	0	55	theme	composite	77:85	arg1	aerogel					87:93	composite aerogel	77:93	composite aerogel for dye adsorption and electrochemical supercapacitor	77:147	Magnetic N-doped carbon aerogel from sodium carboxymethyl cellulose/collagen composite aerogel for dye adsorption and electrochemical supercapacitor.					
29627469	2	56	theme	three-dimensional	495:511	arg1	morphologies					513:524	well-developed porous three-dimensional morphologies	473:524	well-developed porous three-dimensional morphologies	473:524	The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties.					
29627469	2	57	theme	high	527:530	arg1	areas					549:553	high specific surface areas	527:553	high specific surface areas	527:553	The carbon aerogels exhibited well-developed porous three-dimensional morphologies, high specific surface areas, and excellent magnetic properties.					
31199221	10	0	theme	ANI	1395:1397	arg1	methods					1408:1414	the ANI and GGDC methods	1391:1414	methods	1408:1414	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	5	1	theme	major	597:601	arg1	C16 					620:623	C16 	620:623	C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0	620:772	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	1	theme	major	597:601	arg1	acids					609:613	The major fatty acids	593:613	The major fatty acids	593:613	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	11	2	theme	novel	1700:1704	arg1	species					1706:1712	a novel species	1698:1712	a novel species	1698:1712	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	7	3	theme	genus	978:982	arg1	Alteromonas					984:994	the genus Alteromonas	974:994	the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria)	974:1047	Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria).					
31199221	8	4	theme	Strain	1050:1055	arg1	1T					1057:1058	Strain 1T	1050:1058	Strain 1T	1050:1058	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	9	5	theme	strain	1304:1309	arg1	1T					1311:1312	strain 1T	1304:1312	strain 1T	1304:1312	The DNA G+C content of strain 1T was determined to be 43.9 mol%.					
31199221	11	6	theme	genus	1628:1632	arg1	Alteromonas					1634:1644	the genus Alteromonas	1624:1644	the genus Alteromonas with validly published names	1624:1673	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	8	7	theme	rRNA	1074:1077	arg1	similarity					1093:1102	16S rRNA gene sequence similarity	1070:1102	16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas	1070:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	4	8	theme	carbon	577:582	arg1	sources					584:590	sole carbon sources	572:590	sole carbon sources	572:590	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	8	theme	carbon	577:582	arg1	polysaccharides					511:525	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	10	9	theme	other	1438:1442	arg1	members					1444:1450	other members	1438:1450	other members of the genus Alteromonas	1438:1475	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	8	10	theme	sequence	1084:1091	arg1	similarity					1093:1102	16S rRNA gene sequence similarity	1070:1102	16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas	1070:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	6	11	theme	was	799:801	arg1	Q-8					803:805	The respiratory quinone was Q-8	775:805	The respiratory quinone was Q-8	775:805	The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid.					
31199221	6	11	theme	was	799:801	arg1	phosphatidylethanolamine					835:858	phosphatidylethanolamine	835:858	phosphatidylethanolamine	835:858	The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid.					
31199221	5	12	with	feature	652:658	arg1	amounts					716:722	significant amounts	704:722	significant amounts (>6 %) of C16 	704:737	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	12	with	feature	652:658	arg1	%					728:728	>6 %	725:728	>6 %	725:728	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	12	with	feature	652:658	arg1	C16 					734:737	C16 	734:737	C16 	734:737	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	8	13	theme	type	1137:1140	arg1	strains					1142:1148	the type strains	1133:1148	the type strains of Alteromonas mediterranea and Alteromonas australica respectively	1133:1216	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	11	14	theme	published	1659:1667	arg1	names					1669:1673	validly published names	1651:1673	validly published names	1651:1673	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	9	15	theme	43.9 mol	1335:1342	arg1	%					1343:1343	43.9 mol%	1335:1343	43.9 mol%	1335:1343	The DNA G+C content of strain 1T was determined to be 43.9 mol%.					
31199221	9	15	theme	43.9 mol	1335:1342	arg1	G+C content					1289:1299	The DNA G+C content	1281:1299	The DNA G+C content of strain 1T	1281:1312	The DNA G+C content of strain 1T was determined to be 43.9 mol%.					
31199221	6	16	theme	respiratory	779:789	arg1	Q-8					803:805	The respiratory quinone was Q-8	775:805	The respiratory quinone was Q-8	775:805	The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid.					
31199221	6	16	theme	respiratory	779:789	arg1	phosphatidylethanolamine					835:858	phosphatidylethanolamine	835:858	phosphatidylethanolamine	835:858	The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid.					
31199221	10	17	theme	genus	1459:1463	arg1	Alteromonas					1465:1475	the genus Alteromonas	1455:1475	the genus Alteromonas	1455:1475	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	7	18	theme	Phylogenetic	909:920	arg1	analyses					922:929	Phylogenetic analyses	909:929	Phylogenetic analyses	909:929	Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria).					
31199221	1	19	theme	emended	113:119	arg1	description					121:131	emended description	113:131	emended description of the genus Alteromonas	113:156	nov., a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas.					
31199221	8	20	theme	mediterranea	1165:1176	arg1	strains					1142:1148	the type strains	1133:1148	the type strains of Alteromonas mediterranea and Alteromonas australica respectively	1133:1216	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	11	21	theme	strain	1586:1591	arg1	1T					1593:1594	strain 1T	1586:1594	strain 1T	1586:1594	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	2	22	theme	algae	237:241	arg1	thallus					201:207	the thallus	197:207	the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA	197:286	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	8	23	theme	Alteromonas	1182:1192	arg1	australica					1194:1203	Alteromonas australica	1182:1203	Alteromonas australica	1182:1203	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	2	24	theme	carrageenophytic	216:231	arg1	Eucheuma					244:251	Eucheuma	244:251	Eucheuma	244:251	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	2	24	theme	carrageenophytic	216:231	arg1	algae					237:241	the carrageenophytic red algae	212:241	the carrageenophytic red algae	212:241	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	2	24	theme	carrageenophytic	216:231	arg1	USA					284:286	USA	284:286	USA	284:286	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	1	25	theme	genus	140:144	arg1	Alteromonas					146:156	the genus Alteromonas	136:156	the genus Alteromonas	136:156	nov., a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas.					
31199221	4	26	used	used	494:497	arg2	1T					491:492	Strain 1T	484:492	Strain 1T	484:492	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	3	27	dep	Gram-stain-negative	345:363	arg1	aerobic					375:381	aerobic	375:381	aerobic	375:381	Cells were Gram-stain-negative, strictly aerobic, non-flagellated, ovoid or rod-shaped and grew optimally at 20-25 °C, at pH 6-9 and with 2-4 % NaCl.					
31199221	5	28	theme	summed	645:650	arg1	feature					652:658	summed feature 3	645:660	summed feature 3	645:660	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	13	29	theme	111645T=DSM	1849:1859	arg1	1T					1818:1819	1T	1818:1819	1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T)	1818:1868	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	13	29	theme	111645T=DSM	1849:1859	arg1	106819T					1861:1867	=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T	1822:1867	=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T	1822:1867	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	6	30	theme	polar	817:821	arg1	lipids					823:828	major polar lipids	811:828	major polar lipids	811:828	The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid.					
31199221	5	31	with	 1 ω7c	634:639	arg1	amounts					716:722	significant amounts	704:722	significant amounts (>6 %) of C16 	704:737	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	31	with	 1 ω7c	634:639	arg1	%					728:728	>6 %	725:728	>6 %	725:728	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	31	with	 1 ω7c	634:639	arg1	C16 					734:737	C16 	734:737	C16 	734:737	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	11	32	theme	Alteromonas	1727:1737	arg1	species					1706:1712	a novel species	1698:1712	a novel species	1698:1712	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	0	33	theme	Alteromonas	0:10	arg1	sp					19:20	Alteromonas fortis sp	0:20	Alteromonas fortis sp.	0:21	Alteromonas fortis sp.					
31199221	10	34	theme	DNA-DNA	1354:1360	arg1	hybridization					1362:1374	Digital DNA-DNA hybridization	1346:1374	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas	1346:1475	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	5	35	theme	significant	704:714	arg1	amounts					716:722	significant amounts	704:722	significant amounts (>6 %) of C16 	704:737	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	35	theme	significant	704:714	arg1	%					728:728	>6 %	725:728	>6 %	725:728	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	35	theme	significant	704:714	arg1	C16 					734:737	C16 	734:737	C16 	734:737	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	2	36	attach	isolated	170:177	arg1	thallus					201:207	the thallus	197:207	the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA	197:286	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	2	36	attach	isolated	170:177	arg2	1T					166:167	Strain 1T	159:167	Strain 1T	159:167	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	7	37	theme	class	1022:1026	arg1	Alteromonadaceae					1004:1019	family Alteromonadaceae	997:1019	family Alteromonadaceae	997:1019	Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria).					
31199221	7	37	theme	class	1022:1026	arg1	Gammaproteobacteria					1028:1046	class Gammaproteobacteria	1022:1046	class Gammaproteobacteria	1022:1046	Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria).					
31199221	6	38	theme	unknown	889:895	arg1	aminolipid					897:906	an unknown aminolipid	886:906	an unknown aminolipid	886:906	The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid.					
31199221	2	39	theme	Strain	159:164	arg1	1T					166:167	Strain 1T	159:167	Strain 1T	159:167	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	8	40	theme	Alteromonas	1268:1278	arg1	species					1247:1253	other species	1241:1253	other species of the genus Alteromonas	1241:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	13	41	theme	=ATCC	1822:1826	arg1	1T					1818:1819	1T	1818:1819	1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T)	1818:1868	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	13	41	theme	=ATCC	1822:1826	arg1	106819T					1861:1867	=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T	1822:1867	=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T	1822:1867	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	5	42	theme	 0	691:692	arg1	2OH					694:696	 0 2OH	691:696	C16 : 1 ω7c and/or iso-C15 : 0 2OH	663:696	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	13	43	theme	5933T=CIP	1839:1847	arg1	1T					1818:1819	1T	1818:1819	1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T)	1818:1868	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	13	43	theme	5933T=CIP	1839:1847	arg1	106819T					1861:1867	=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T	1822:1867	=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T	1822:1867	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	1	44	theme	non-flagellated	31:45	arg1	bacterium					47:55	a non-flagellated bacterium	29:55	a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas	29:156	nov., a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas.					
31199221	1	44	theme	non-flagellated	31:45	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas.					
31199221	2	45	theme	polyphasic	315:324	arg1	method					326:331	a polyphasic method	313:331	a polyphasic method	313:331	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	5	46	theme	fatty	603:607	arg1	C16 					620:623	C16 	620:623	C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0	620:772	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	46	theme	fatty	603:607	arg1	acids					609:613	The major fatty acids	593:613	The major fatty acids	593:613	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	9	47	theme	DNA	1285:1287	arg1	%					1343:1343	43.9 mol%	1335:1343	43.9 mol%	1335:1343	The DNA G+C content of strain 1T was determined to be 43.9 mol%.					
31199221	9	47	theme	DNA	1285:1287	arg1	G+C content					1289:1299	The DNA G+C content	1281:1299	The DNA G+C content of strain 1T	1281:1312	The DNA G+C content of strain 1T was determined to be 43.9 mol%.					
31199221	10	48	theme	strain	1424:1429	arg1	1T					1431:1432	strain 1T	1424:1432	strain 1T	1424:1432	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	3	49	theme	%	476:476	arg1	 NaCl					477:481	2-4 % NaCl	472:481	2-4 % NaCl	472:481	Cells were Gram-stain-negative, strictly aerobic, non-flagellated, ovoid or rod-shaped and grew optimally at 20-25 °C, at pH 6-9 and with 2-4 % NaCl.					
31199221	9	50	theme	1T	1311:1312	arg1	%					1343:1343	43.9 mol%	1335:1343	43.9 mol%	1335:1343	The DNA G+C content of strain 1T was determined to be 43.9 mol%.					
31199221	9	50	theme	1T	1311:1312	arg1	G+C content					1289:1299	The DNA G+C content	1281:1299	The DNA G+C content of strain 1T	1281:1312	The DNA G+C content of strain 1T was determined to be 43.9 mol%.					
31199221	5	51	theme	C16 	734:737	arg1	amounts					716:722	significant amounts	704:722	significant amounts (>6 %) of C16 	704:737	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	51	theme	C16 	734:737	arg1	%					728:728	>6 %	725:728	>6 %	725:728	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	51	theme	C16 	734:737	arg1	C16 					734:737	C16 	734:737	C16 	734:737	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	6	52	theme	major	811:815	arg1	lipids					823:828	major polar lipids	811:828	major polar lipids	811:828	The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid.					
31199221	7	53	dep	Alteromonas	984:994	arg1	Alteromonadaceae					1004:1019	family Alteromonadaceae	997:1019	family Alteromonadaceae	997:1019	Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria).					
31199221	7	53	dep	Alteromonas	984:994	arg1	Gammaproteobacteria					1028:1046	class Gammaproteobacteria	1022:1046	class Gammaproteobacteria	1022:1046	Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria).					
31199221	11	54	theme	Alteromonas	1634:1644	arg1	species					1613:1619	species	1613:1619	species of the genus Alteromonas with validly published names	1613:1673	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	8	55	theme	16S	1070:1072	arg1	similarity					1093:1102	16S rRNA gene sequence similarity	1070:1102	16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas	1070:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	4	56	theme	sole	572:575	arg1	sources					584:590	sole carbon sources	572:590	sole carbon sources	572:590	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	56	theme	sole	572:575	arg1	polysaccharides					511:525	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	8	57	theme	gene	1079:1082	arg1	similarity					1093:1102	16S rRNA gene sequence similarity	1070:1102	16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas	1070:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	6	58	theme	quinone	791:797	arg1	Q-8					803:805	The respiratory quinone was Q-8	775:805	The respiratory quinone was Q-8	775:805	The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid.					
31199221	6	58	theme	quinone	791:797	arg1	phosphatidylethanolamine					835:858	phosphatidylethanolamine	835:858	phosphatidylethanolamine	835:858	The respiratory quinone was Q-8 and major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unknown aminolipid.					
31199221	5	59	dep	C16 	663:666	arg1	iso-C15 					682:689	iso-C15 	682:689	iso-C15 	682:689	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	59	dep	C16 	663:666	arg1	2OH					694:696	 0 2OH	691:696	C16 : 1 ω7c and/or iso-C15 : 0 2OH	663:696	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	59	dep	C16 	663:666	arg1	 1 ω7c					668:673	 1 ω7c	668:673	 1 ω7c	668:673	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	10	60	theme	Alteromonas	1465:1475	arg1	1T					1431:1432	strain 1T	1424:1432	strain 1T	1424:1432	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	10	60	theme	Alteromonas	1465:1475	arg1	members					1444:1450	other members	1438:1450	other members of the genus Alteromonas	1438:1475	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	4	61	dep	polysaccharides	511:525	arg1	polysaccharides					511:525	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	61	dep	polysaccharides	511:525	arg1	laminarin					542:550	laminarin	542:550	laminarin	542:550	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	61	dep	polysaccharides	511:525	arg1	acid					564:567	alginic acid	556:567	alginic acid	556:567	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	61	dep	polysaccharides	511:525	arg1	ι-carrageenan					527:539	ι-carrageenan	527:539	ι-carrageenan	527:539	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	11	62	theme	Alteromonasfortis	1759:1775	arg1	sp					1777:1778	the name Alteromonasfortis sp	1750:1778	the name Alteromonasfortis sp	1750:1778	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	5	63	theme	methyl	759:764	arg1	C17 					766:769	 0 N alcohol and 10 methyl C17 	739:769	C17 	766:769	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	8	64	theme	similarity	1093:1102	arg1	values					1104:1109	16S rRNA gene sequence similarity values	1070:1109	16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas	1070:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	8	65	theme	Alteromonas	1153:1163	arg1	mediterranea					1165:1176	Alteromonas mediterranea	1153:1176	Alteromonas mediterranea	1153:1176	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	2	66	theme	red	233:235	arg1	Eucheuma					244:251	Eucheuma	244:251	Eucheuma	244:251	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	2	66	theme	red	233:235	arg1	algae					237:241	the carrageenophytic red algae	212:241	the carrageenophytic red algae	212:241	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	2	66	theme	red	233:235	arg1	USA					284:286	USA	284:286	USA	284:286	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	5	67	dep	 1 ω7c	634:639	arg1	C16 					663:666	C16 	663:666	C16 : 1 ω7c and/or iso-C15 : 0 2OH	663:696	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	2	68	dep	Eucheuma	244:251	arg1	spinosum					253:260	Eucheuma spinosum	244:260	Eucheuma spinosum	244:260	Strain 1T, isolated in the 1970s from the thallus of the carrageenophytic red algae, Eucheuma spinosum, collected in Hawaii, USA, was characterized using a polyphasic method.					
31199221	1	69	theme	Alteromonas	146:156	arg1	description					121:131	emended description	113:131	emended description of the genus Alteromonas	113:156	nov., a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas.					
31199221	1	69	theme	Alteromonas	146:156	arg1	degradation					76:86	the degradation	72:86	the degradation of iota-carrageenan	72:106	nov., a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas.					
31199221	8	70	theme	australica	1194:1203	arg1	strains					1142:1148	the type strains	1133:1148	the type strains of Alteromonas mediterranea and Alteromonas australica respectively	1133:1216	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	8	71	theme	%	1128:1128	arg1	values					1104:1109	16S rRNA gene sequence similarity values	1070:1109	16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas	1070:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	5	72	dep	C16 	620:623	arg1	C17 					766:769	 0 N alcohol and 10 methyl C17 	739:769	C17 	766:769	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	72	dep	C16 	620:623	arg1	 0					771:772	 0	771:772	C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0	620:772	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	72	dep	C16 	620:623	arg1	 1 ω7c					634:639	 1 ω7c	634:639	 1 ω7c	634:639	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	72	dep	C16 	620:623	arg1	alcohol					744:750	 0 N alcohol and 10 methyl C17 	739:769	alcohol	744:750	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	72	dep	C16 	620:623	arg1	 0					625:626	 0	625:626	 0	625:626	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	72	dep	C16 	620:623	arg1	feature					652:658	summed feature 3	645:660	summed feature 3	645:660	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	5	72	dep	C16 	620:623	arg1	C18 					629:632	C18 	629:632	C18 	629:632	The major fatty acids were C16 : 0, C18 : 1 ω7c and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2OH) with significant amounts (>6 %) of C16 : 0 N alcohol and 10 methyl C17 : 0.					
31199221	0	73	theme	fortis	12:17	arg1	sp					19:20	Alteromonas fortis sp	0:20	Alteromonas fortis sp.	0:21	Alteromonas fortis sp.					
31199221	8	74	theme	%	1236:1236	arg1	values					1104:1109	16S rRNA gene sequence similarity values	1070:1109	16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas	1070:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	11	75	theme	genus	1721:1725	arg1	Alteromonas					1727:1737	the genus Alteromonas	1717:1737	the genus Alteromonas	1717:1737	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	11	76	with	Alteromonas	1634:1644	arg1	names					1669:1673	validly published names	1651:1673	validly published names	1651:1673	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	1	77	theme	iota-carrageenan	91:106	arg1	description					121:131	emended description	113:131	emended description of the genus Alteromonas	113:156	nov., a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas.					
31199221	1	77	theme	iota-carrageenan	91:106	arg1	degradation					76:86	the degradation	72:86	the degradation of iota-carrageenan	72:106	nov., a non-flagellated bacterium specialized in the degradation of iota-carrageenan, and emended description of the genus Alteromonas.					
31199221	4	78	theme	seaweed	503:509	arg1	sources					584:590	sole carbon sources	572:590	sole carbon sources	572:590	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	78	theme	seaweed	503:509	arg1	polysaccharides					511:525	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	78	theme	seaweed	503:509	arg1	laminarin					542:550	laminarin	542:550	laminarin	542:550	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	78	theme	seaweed	503:509	arg1	acid					564:567	alginic acid	556:567	alginic acid	556:567	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	78	theme	seaweed	503:509	arg1	ι-carrageenan					527:539	ι-carrageenan	527:539	ι-carrageenan	527:539	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	10	79	theme	Digital	1346:1352	arg1	hybridization					1362:1374	Digital DNA-DNA hybridization	1346:1374	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas	1346:1475	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	11	80	theme	phenotypic	1530:1539	arg1	analyses					1567:1574	The phenotypic, phylogenetic and genomic analyses	1526:1574	The phenotypic, phylogenetic and genomic analyses	1526:1574	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	8	81	theme	other	1241:1245	arg1	species					1247:1253	other species	1241:1253	other species of the genus Alteromonas	1241:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	11	82	theme	name	1754:1757	arg1	sp					1777:1778	the name Alteromonasfortis sp	1750:1778	the name Alteromonasfortis sp	1750:1778	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	7	83	theme	family	997:1002	arg1	Alteromonadaceae					1004:1019	family Alteromonadaceae	997:1019	family Alteromonadaceae	997:1019	Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria).					
31199221	7	83	theme	family	997:1002	arg1	Gammaproteobacteria					1028:1046	class Gammaproteobacteria	1022:1046	class Gammaproteobacteria	1022:1046	Phylogenetic analyses showed that the bacterium is affiliated to the genus Alteromonas (family Alteromonadaceae, class Gammaproteobacteria).					
31199221	11	84	from	species	1613:1619	arg1	distinct					1599:1606	distinct	1599:1606	distinct	1599:1606	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	4	85	theme	Strain	484:489	arg1	1T					491:492	Strain 1T	484:492	Strain 1T	484:492	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	11	86	theme	genomic	1559:1565	arg1	analyses					1567:1574	The phenotypic, phylogenetic and genomic analyses	1526:1574	The phenotypic, phylogenetic and genomic analyses	1526:1574	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	8	87	theme	genus	1262:1266	arg1	Alteromonas					1268:1278	the genus Alteromonas	1258:1278	the genus Alteromonas	1258:1278	Strain 1T exhibited 16S rRNA gene sequence similarity values of 98.8 and 99.2 % to the type strains of Alteromonas mediterranea and Alteromonas australica respectively, and of 95.2-98.6 % to other species of the genus Alteromonas.					
31199221	13	88	theme	43554T=RCC	1828:1837	arg1	1T					1818:1819	1T	1818:1819	1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T)	1818:1868	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	13	88	theme	43554T=RCC	1828:1837	arg1	106819T					1861:1867	=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T	1822:1867	=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T	1822:1867	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	4	89	theme	alginic	556:562	arg1	polysaccharides					511:525	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid	499:567	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	4	89	theme	alginic	556:562	arg1	acid					564:567	alginic acid	556:567	alginic acid	556:567	Strain 1T used the seaweed polysaccharides ι-carrageenan, laminarin and alginic acid as sole carbon sources.					
31199221	10	90	theme	hybridization	1362:1374	arg1	predictions					1376:1386	Digital DNA-DNA hybridization predictions	1346:1386	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas	1346:1475	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	11	91	theme	phylogenetic	1542:1553	arg1	analyses					1567:1574	The phenotypic, phylogenetic and genomic analyses	1526:1574	The phenotypic, phylogenetic and genomic analyses	1526:1574	The phenotypic, phylogenetic and genomic analyses show that strain 1T is distinct from species of the genus Alteromonas with validly published names and that it represents a novel species of the genus Alteromonas, for which the name Alteromonasfortis sp.					
31199221	13	92	theme	type	1803:1806	arg1	1T					1818:1819	1T	1818:1819	1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T)	1818:1868	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	13	92	theme	type	1803:1806	arg1	strain					1808:1813	The type strain	1799:1813	The type strain	1799:1813	The type strain is 1T (=ATCC 43554T=RCC 5933T=CIP 111645T=DSM 106819T).					
31199221	10	93	theme	GGDC	1403:1406	arg1	methods					1408:1414	the ANI and GGDC methods	1391:1414	methods	1408:1414	Digital DNA-DNA hybridization predictions by the ANI and GGDC methods between strain 1T and other members of the genus Alteromonas showed values below 83 % and 30 %, respectively.					
31199221	3	94	theme	2-4 	472:475	arg1	 NaCl					477:481	2-4 % NaCl	472:481	2-4 % NaCl	472:481	Cells were Gram-stain-negative, strictly aerobic, non-flagellated, ovoid or rod-shaped and grew optimally at 20-25 °C, at pH 6-9 and with 2-4 % NaCl.					
29803429	8	0	from	extraction	1513:1522	arg1	plants					1569:1574	Aristolochiaceae plants	1552:1574	Aristolochiaceae plants	1552:1574	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	5	1	theme	chitosan	967:974	arg1	microcoils					992:1001	The chitosan modified carbon microcoils	963:1001	The chitosan modified carbon microcoils	963:1001	The chitosan modified carbon microcoils exhibited excellent binding ability (77.72 mg g-1) and satisfactory selectivity.					
29803429	4	2	theme	microcoils	951:960	arg1	selectivity					916:926	selectivity	916:926	selectivity	916:926	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	4	2	theme	microcoils	951:960	arg1	capacity					903:910	adsorption capacity	892:910	adsorption capacity	892:910	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	7	3	dep	150 mg L-1	1294:1303	arg1	to					1291:1292	to	1291:1292	to	1291:1292	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	2	4	theme	selective	381:389	arg1	separation					391:400	the selective separation	377:400	the selective separation of aristolochic acid I from medicinal herbs	377:444	In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from medicinal herbs.					
29803429	1	5	theme	medicinal	277:285	arg1	plants					287:292	Aristolochiaceae medicinal plants	260:292	Aristolochiaceae medicinal plants	260:292	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	8	6	from	plants	1569:1574	arg1	extraction					1513:1522	the selective extraction	1499:1522	the selective extraction of aristolochic acid I from Aristolochiaceae plants	1499:1574	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	8	6	from	plants	1569:1574	arg1	acid					1540:1543	aristolochic acid I	1527:1545	aristolochic acid I from Aristolochiaceae plants	1527:1574	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	4	7	theme	carbon	944:949	arg1	microcoils					951:960	the modified carbon microcoils	931:960	the modified carbon microcoils	931:960	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	1	8	from	compound	173:180	arg1	plants					287:292	Aristolochiaceae medicinal plants	260:292	Aristolochiaceae medicinal plants	260:292	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	4	9	theme	acid	778:781	arg1	adsorption					751:760	the adsorption	747:760	the adsorption of aristolochic acid I	747:783	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	5	10	theme	modified	976:983	arg1	microcoils					992:1001	The chitosan modified carbon microcoils	963:1001	The chitosan modified carbon microcoils	963:1001	The chitosan modified carbon microcoils exhibited excellent binding ability (77.72 mg g-1) and satisfactory selectivity.					
29803429	0	11	theme	medicinal	118:126	arg1	plants					128:133	Aristolochiaceae medicinal plants	101:133	Aristolochiaceae medicinal plants	101:133	Hybrid-type carbon microcoil-chitosan composite for selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants.					
29803429	7	12	theme	%	1428:1428	arg1	deviations					1403:1412	the relative standard deviations	1381:1412	the relative standard deviations of less than 5%	1381:1428	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	2	13	theme	chitosan	310:317	arg1	microcoils					335:344	chitosan modified carbon microcoils	310:344	chitosan modified carbon microcoils	310:344	In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from medicinal herbs.					
29803429	0	14	theme	Aristolochiaceae	101:116	arg1	plants					128:133	Aristolochiaceae medicinal plants	101:133	Aristolochiaceae medicinal plants	101:133	Hybrid-type carbon microcoil-chitosan composite for selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants.					
29803429	7	15	theme	aristolochic	1328:1339	arg1	acid					1341:1344	aristolochic acid I	1328:1346	aristolochic acid I	1328:1346	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	3	16	theme	photoelectron	605:617	arg1	spectroscopy					619:630	X-ray photoelectron spectroscopy	599:630	X-ray photoelectron spectroscopy	599:630	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	6	17	theme	medicinal	1207:1215	arg1	plants					1217:1222	medicinal plants	1207:1222	medicinal plants	1207:1222	Finally, this material was used in solid phase extraction combined with HPLC to enrich and detect aristolochic acid I from medicinal plants.					
29803429	6	18	used	used	1111:1114	arg2	material					1098:1105	this material	1093:1105	this material	1093:1105	Finally, this material was used in solid phase extraction combined with HPLC to enrich and detect aristolochic acid I from medicinal plants.					
29803429	3	19	theme	scanning	509:516	arg1	microscopy					527:536	scanning electron microscopy	509:536	scanning electron microscopy	509:536	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	6	20	theme	phase	1125:1129	arg1	extraction					1131:1140	solid phase extraction	1119:1140	solid phase extraction combined with HPLC	1119:1159	Finally, this material was used in solid phase extraction combined with HPLC to enrich and detect aristolochic acid I from medicinal plants.					
29803429	1	21	theme	many	201:204	arg1	kinds					206:210	many kinds	201:210	many kinds of traditional Chinese medicines	201:243	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	3	22	theme	carbon	474:479	arg1	microcoils					481:490	carbon microcoils	474:490	carbon microcoils	474:490	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	4	23	theme	selectivity	843:853	arg1	tests					855:859	selectivity tests	843:859	selectivity tests	843:859	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	6	24	theme	aristolochic	1182:1193	arg1	acid					1195:1198	aristolochic acid I	1182:1200	aristolochic acid I from medicinal plants	1182:1222	Finally, this material was used in solid phase extraction combined with HPLC to enrich and detect aristolochic acid I from medicinal plants.					
29803429	3	25	theme	microcoils	481:490	arg1	modification					458:469	Successful modification	447:469	Successful modification of carbon microcoils	447:490	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	8	26	theme	ideal	1478:1482	arg1	adsorbents					1484:1493	ideal adsorbents	1478:1493	ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants	1478:1574	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	4	27	theme	modified	935:942	arg1	microcoils					951:960	the modified carbon microcoils	931:960	the modified carbon microcoils	931:960	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	2	28	theme	acid	418:421	arg1	separation					391:400	the selective separation	377:400	the selective separation of aristolochic acid I from medicinal herbs	377:444	In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from medicinal herbs.					
29803429	0	29	theme	Hybrid-type	0:10	arg1	carbon					12:17	Hybrid-type carbon	0:17	Hybrid-type carbon	0:17	Hybrid-type carbon microcoil-chitosan composite for selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants.					
29803429	3	30	theme	thermogravimetric	637:653	arg1	analyses					655:662	thermogravimetric analyses	637:662	thermogravimetric analyses	637:662	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	2	31	theme	aristolochic	405:416	arg1	acid					418:421	aristolochic acid I	405:423	aristolochic acid I	405:423	In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from medicinal herbs.					
29803429	5	32	theme	carbon	985:990	arg1	microcoils					992:1001	The chitosan modified carbon microcoils	963:1001	The chitosan modified carbon microcoils	963:1001	The chitosan modified carbon microcoils exhibited excellent binding ability (77.72 mg g-1) and satisfactory selectivity.					
29803429	2	33	theme	carbon	328:333	arg1	microcoils					335:344	chitosan modified carbon microcoils	310:344	chitosan modified carbon microcoils	310:344	In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from medicinal herbs.					
29803429	8	34	theme	carbon	1455:1460	arg1	microcoils					1462:1471	chitosan modified carbon microcoils	1437:1471	chitosan modified carbon microcoils	1437:1471	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	5	35	theme	satisfactory	1058:1069	arg1	selectivity					1071:1081	satisfactory selectivity	1058:1081	satisfactory selectivity	1058:1081	The chitosan modified carbon microcoils exhibited excellent binding ability (77.72 mg g-1) and satisfactory selectivity.					
29803429	4	36	theme	Adsorption	807:816	arg1	isotherms					818:826	Adsorption isotherms	807:826	Adsorption isotherms	807:826	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	2	37	theme	modified	319:326	arg1	microcoils					335:344	chitosan modified carbon microcoils	310:344	chitosan modified carbon microcoils	310:344	In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from medicinal herbs.					
29803429	1	38	theme	traditional	215:225	arg1	medicines					235:243	traditional Chinese medicines	215:243	traditional Chinese medicines	215:243	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	0	39	theme	selective	52:60	arg1	extraction					62:71	selective extraction	52:71	selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants	52:133	Hybrid-type carbon microcoil-chitosan composite for selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants.					
29803429	5	40	theme	excellent	1013:1021	arg1	77.72 mg g-1					1040:1051	77.72 mg g-1	1040:1051	77.72 mg g-1	1040:1051	The chitosan modified carbon microcoils exhibited excellent binding ability (77.72 mg g-1) and satisfactory selectivity.					
29803429	5	40	theme	excellent	1013:1021	arg1	ability					1031:1037	excellent binding ability	1013:1037	excellent binding ability (77.72 mg g-1)	1013:1052	The chitosan modified carbon microcoils exhibited excellent binding ability (77.72 mg g-1) and satisfactory selectivity.					
29803429	7	41	theme	acid	1341:1344	arg1	recoveries					1314:1323	the recoveries	1310:1323	the recoveries of aristolochic acid I	1310:1346	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	4	42	theme	adsorption	892:901	arg1	capacity					903:910	adsorption capacity	892:910	adsorption capacity	892:910	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	7	43	theme	standard	1394:1401	arg1	deviations					1403:1412	the relative standard deviations	1381:1412	the relative standard deviations of less than 5%	1381:1428	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	1	44	theme	Chinese	227:233	arg1	medicines					235:243	traditional Chinese medicines	215:243	traditional Chinese medicines	215:243	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	3	45	theme	Successful	447:456	arg1	modification					458:469	Successful modification	447:469	Successful modification of carbon microcoils	447:490	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	7	46	theme	aristolochic	1251:1262	arg1	acid					1264:1267	aristolochic acid I	1251:1269	aristolochic acid I	1251:1269	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	8	47	theme	aristolochic	1527:1538	arg1	acid					1540:1543	aristolochic acid I	1527:1545	aristolochic acid I from Aristolochiaceae plants	1527:1574	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	8	48	theme	modified	1446:1453	arg1	microcoils					1462:1471	chitosan modified carbon microcoils	1437:1471	chitosan modified carbon microcoils	1437:1471	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	6	49	from	plants	1217:1222	arg1	acid					1195:1198	aristolochic acid I	1182:1200	aristolochic acid I from medicinal plants	1182:1222	Finally, this material was used in solid phase extraction combined with HPLC to enrich and detect aristolochic acid I from medicinal plants.					
29803429	1	50	theme	medicines	235:243	arg1	kinds					206:210	many kinds	201:210	many kinds of traditional Chinese medicines	201:243	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	7	51	theme	detector	1229:1236	arg1	response					1238:1245	The detector response	1225:1245	The detector response for aristolochic acid I	1225:1269	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	7	51	theme	detector	1229:1236	arg1	linear					1275:1280	linear	1275:1280	linear	1275:1280	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	3	52	theme	infrared	556:563	arg1	spectroscopy					565:576	Fourier-transfer infrared spectroscopy	539:576	Fourier-transfer infrared spectroscopy	539:576	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	7	53	from	150 mg L-1	1294:1303	arg1	response					1238:1245	The detector response	1225:1245	The detector response for aristolochic acid I	1225:1269	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	7	53	from	150 mg L-1	1294:1303	arg1	linear					1275:1280	linear	1275:1280	linear	1275:1280	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	1	54	theme	Aristolochic	136:147	arg1	compound					173:180	a nephrotoxic compound	159:180	a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines	159:243	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	1	54	theme	Aristolochic	136:147	arg1	acid					149:152	Aristolochic acid I	136:154	Aristolochic acid I	136:154	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	4	55	dep	capacity	903:910	arg1	the					888:890	the	888:890	the	888:890	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	8	56	theme	acid	1540:1543	arg1	extraction					1513:1522	the selective extraction	1499:1522	the selective extraction of aristolochic acid I from Aristolochiaceae plants	1499:1574	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	3	57	theme	Fourier-transfer	539:554	arg1	spectroscopy					565:576	Fourier-transfer infrared spectroscopy	539:576	Fourier-transfer infrared spectroscopy	539:576	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	8	58	theme	selective	1503:1511	arg1	extraction					1513:1522	the selective extraction	1499:1522	the selective extraction of aristolochic acid I from Aristolochiaceae plants	1499:1574	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	0	59	from	plants	128:133	arg1	extraction					62:71	selective extraction	52:71	selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants	52:133	Hybrid-type carbon microcoil-chitosan composite for selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants.					
29803429	7	60	dep	77.73	1369:1373	arg1	to					1366:1367	to	1366:1367	to	1366:1367	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	6	61	theme	solid	1119:1123	arg1	extraction					1131:1140	solid phase extraction	1119:1140	solid phase extraction combined with HPLC	1119:1159	Finally, this material was used in solid phase extraction combined with HPLC to enrich and detect aristolochic acid I from medicinal plants.					
29803429	3	62	theme	electron	518:525	arg1	microscopy					527:536	scanning electron microscopy	509:536	scanning electron microscopy	509:536	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	8	63	theme	chitosan	1437:1444	arg1	microcoils					1462:1471	chitosan modified carbon microcoils	1437:1471	chitosan modified carbon microcoils	1437:1471	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	4	64	theme	adsorption	680:689	arg1	conditions					691:700	adsorption conditions	680:700	adsorption conditions	680:700	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	5	65	theme	binding	1023:1029	arg1	77.72 mg g-1					1040:1051	77.72 mg g-1	1040:1051	77.72 mg g-1	1040:1051	The chitosan modified carbon microcoils exhibited excellent binding ability (77.72 mg g-1) and satisfactory selectivity.					
29803429	5	65	theme	binding	1023:1029	arg1	ability					1031:1037	excellent binding ability	1013:1037	excellent binding ability (77.72 mg g-1)	1013:1052	The chitosan modified carbon microcoils exhibited excellent binding ability (77.72 mg g-1) and satisfactory selectivity.					
29803429	8	66	theme	Aristolochiaceae	1552:1567	arg1	plants					1569:1574	Aristolochiaceae plants	1552:1574	Aristolochiaceae plants	1552:1574	Thus, chitosan modified carbon microcoils were ideal adsorbents for the selective extraction of aristolochic acid I from Aristolochiaceae plants.					
29803429	3	67	theme	X-ray	599:603	arg1	spectroscopy					619:630	X-ray photoelectron spectroscopy	599:630	X-ray photoelectron spectroscopy	599:630	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	2	68	from	herbs	440:444	arg1	separation					391:400	the selective separation	377:400	the selective separation of aristolochic acid I from medicinal herbs	377:444	In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from medicinal herbs.					
29803429	3	69	theme	elemental	579:587	arg1	analysis					589:596	elemental analysis	579:596	elemental analysis	579:596	Successful modification of carbon microcoils was confirmed by scanning electron microscopy, Fourier-transfer infrared spectroscopy, elemental analysis, X-ray photoelectron spectroscopy, and thermogravimetric analyses.					
29803429	7	70	theme	relative	1385:1392	arg1	deviations					1403:1412	the relative standard deviations	1381:1412	the relative standard deviations of less than 5%	1381:1428	The detector response for aristolochic acid I was linear from 0.5 to 150 mg L-1, and the recoveries of aristolochic acid I ranged from 73.61 to 77.73% with the relative standard deviations of less than 5%.					
29803429	4	71	dep	isotherms	818:826	arg1	pH.					803:805	pH. Adsorption isotherms, kinetics, and selectivity tests	803:859	pH. Adsorption isotherms, kinetics, and selectivity tests	803:859	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	0	72	theme	acid	89:92	arg1	extraction					62:71	selective extraction	52:71	selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants	52:133	Hybrid-type carbon microcoil-chitosan composite for selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants.					
29803429	2	73	theme	medicinal	430:438	arg1	herbs					440:444	medicinal herbs	430:444	medicinal herbs	430:444	In this study, chitosan modified carbon microcoils were designed and prepared for the selective separation of aristolochic acid I from medicinal herbs.					
29803429	4	74	theme	aristolochic	765:776	arg1	acid					778:781	aristolochic acid I	765:783	aristolochic acid I	765:783	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	0	75	theme	aristolochic	76:87	arg1	acid					89:92	aristolochic acid I	76:94	aristolochic acid I	76:94	Hybrid-type carbon microcoil-chitosan composite for selective extraction of aristolochic acid I from Aristolochiaceae medicinal plants.					
29803429	4	76	theme	conditions	691:700	arg1	effects					669:675	The effects	665:675	The effects of adsorption conditions	665:700	The effects of adsorption conditions were investigated and it was determined that the adsorption of aristolochic acid I was controlled by pH. Adsorption isotherms, kinetics, and selectivity tests were performed to evaluate the adsorption capacity and selectivity of the modified carbon microcoils.					
29803429	1	77	theme	nephrotoxic	161:171	arg1	compound					173:180	a nephrotoxic compound	159:180	a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines	159:243	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	1	77	theme	nephrotoxic	161:171	arg1	acid					149:152	Aristolochic acid I	136:154	Aristolochic acid I	136:154	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29803429	1	78	theme	Aristolochiaceae	260:275	arg1	plants					287:292	Aristolochiaceae medicinal plants	260:292	Aristolochiaceae medicinal plants	260:292	Aristolochic acid I is a nephrotoxic compound widely existing in many kinds of traditional Chinese medicines, especially in Aristolochiaceae medicinal plants.					
29569870	0	0	theme	electronic	169:178	arg1	microscopy					180:189	environmental scanning electronic microscopy	146:189	environmental scanning electronic microscopy	146:189	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	5	1	located	found	905:909	arg1	oats					914:917	oats	914:917	oats	914:917	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	5	1	located	found	905:909	arg2	granules					891:898	irregular starchy granules	873:898	irregular starchy granules	873:898	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	4	2	theme	type	703:706	arg1	layer					682:686	The aleurone layer	669:686	The aleurone layer of each cereal type	669:706	The aleurone layer of each cereal type contained protein body globoids within its cells.					
29569870	1	3	theme	Electron	241:248	arg1	ESEM-EDS					293:300	ESEM-EDS	293:300	ESEM-EDS	293:300	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	3	theme	Electron	241:248	arg1	Spectroscopy					279:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy	218:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS)	218:301	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	0	4	theme	layer	64:68	arg1	insights					6:13	Novel insights	0:13	Novel insights into pericarp	0:27	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	0	4	theme	layer	64:68	arg1	globoids					43:50	protein body globoids	30:50	protein body globoids of aleurone layer	30:68	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	0	5	theme	scanning	160:167	arg1	microscopy					180:189	environmental scanning electronic microscopy	146:189	environmental scanning electronic microscopy	146:189	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	4	6	theme	cereal	696:701	arg1	type					703:706	each cereal type	691:706	each cereal type	691:706	The aleurone layer of each cereal type contained protein body globoids within its cells.					
29569870	9	7	theme	morphological	1453:1465	arg1	makeup					1480:1485	the morphological and chemical makeup	1449:1485	the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats	1449:1607	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	1	8	theme	Microscopy-Energy	250:266	arg1	ESEM-EDS					293:300	ESEM-EDS	293:300	ESEM-EDS	293:300	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	8	theme	Microscopy-Energy	250:266	arg1	Spectroscopy					279:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy	218:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS)	218:301	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	9	9	theme	important	1620:1628	arg1	suggestions					1630:1640	important suggestions	1620:1640	important suggestions	1620:1640	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	5	10	dep	revealed	810:817	arg1	whereas					865:871	whereas	865:871	whereas	865:871	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	1	11	theme	Dispersive	268:277	arg1	ESEM-EDS					293:300	ESEM-EDS	293:300	ESEM-EDS	293:300	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	11	theme	Dispersive	268:277	arg1	Spectroscopy					279:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy	218:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS)	218:301	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	0	12	theme	starchy	71:77	arg1	granules					79:86	starchy granules	71:86	starchy granules of three cereals	71:103	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	0	12	theme	starchy	71:77	arg1	insights					6:13	Novel insights	0:13	Novel insights into pericarp	0:27	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	8	13	dep	one	1396:1398	arg1	another					1400:1406	another	1400:1406	another	1400:1406	Regarding the topographic profiles, detected by AFM, einkorn wheat starchy granules showed a surface profile that differed significantly from that of oats and barley, which were quite similar to one another.					
29569870	7	14	theme	protein	1105:1111	arg1	globoids					1118:1125	the protein body globoids	1101:1125	the protein body globoids	1101:1125	In the protein body globoids, oats showed higher P and K concentrations than barley and einkorn wheat.					
29569870	6	15	theme	granule	932:938	arg1	composition					950:960	The starchy granule elemental composition	920:960	The starchy granule elemental composition	920:960	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	6	15	theme	granule	932:938	arg1	homogenous					996:1005	homogenous	996:1005	homogenous	996:1005	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	2	16	theme	cereals	516:522	arg1	structures					432:441	The morphological structures	414:441	The morphological structures	414:441	The morphological structures, chemical elemental composition and surface characteristics of the three cereals were described.					
29569870	2	16	theme	cereals	516:522	arg1	composition					463:473	chemical elemental composition	444:473	chemical elemental composition	444:473	The morphological structures, chemical elemental composition and surface characteristics of the three cereals were described.					
29569870	2	16	theme	cereals	516:522	arg1	characteristics					487:501	surface characteristics	479:501	surface characteristics	479:501	The morphological structures, chemical elemental composition and surface characteristics of the three cereals were described.					
29569870	1	17	theme	different	355:363	arg1	oat					391:393	oat	391:393	oat	391:393	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	17	theme	different	355:363	arg1	wheat					407:411	einkorn wheat	399:411	einkorn wheat	399:411	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	17	theme	different	355:363	arg1	barley					383:388	barley	383:388	barley	383:388	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	17	theme	different	355:363	arg1	caryopses					372:380	three different cereal caryopses	349:380	three different cereal caryopses: barley, oat and einkorn wheat	349:411	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	9	18	theme	human	1646:1650	arg1	nutrition					1652:1660	human nutrition	1646:1660	human nutrition	1646:1660	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	9	19	theme	present	1413:1419	arg1	work					1421:1424	The present work	1409:1424	The present work	1409:1424	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	5	20	theme	wheat	858:862	arg1	endosperm					826:834	the endosperm	822:834	the endosperm of barley and einkorn wheat	822:862	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	2	21	theme	chemical	444:451	arg1	composition					463:473	chemical elemental composition	444:473	chemical elemental composition	444:473	The morphological structures, chemical elemental composition and surface characteristics of the three cereals were described.					
29569870	1	22	theme	cereal	365:370	arg1	oat					391:393	oat	391:393	oat	391:393	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	22	theme	cereal	365:370	arg1	wheat					407:411	einkorn wheat	399:411	einkorn wheat	399:411	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	22	theme	cereal	365:370	arg1	barley					383:388	barley	383:388	barley	383:388	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	22	theme	cereal	365:370	arg1	caryopses					372:380	three different cereal caryopses	349:380	three different cereal caryopses: barley, oat and einkorn wheat	349:411	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	5	23	theme	type-B	781:786	arg1	starchy					788:794	small type-B starchy	775:794	small type-B starchy	775:794	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	8	24	theme	starchy	1268:1274	arg1	granules					1276:1283	einkorn wheat starchy granules	1254:1283	einkorn wheat starchy granules	1254:1283	Regarding the topographic profiles, detected by AFM, einkorn wheat starchy granules showed a surface profile that differed significantly from that of oats and barley, which were quite similar to one another.					
29569870	6	25	theme	body	1062:1065	arg1	globoids					1067:1074	the pericarp and protein body globoids	1037:1074	globoids	1067:1074	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	7	26	theme	K	1153:1153	arg1	concentrations					1155:1168	P and K concentrations	1147:1168	P and K concentrations	1147:1168	In the protein body globoids, oats showed higher P and K concentrations than barley and einkorn wheat.					
29569870	2	27	theme	surface	479:485	arg1	characteristics					487:501	surface characteristics	479:501	surface characteristics	479:501	The morphological structures, chemical elemental composition and surface characteristics of the three cereals were described.					
29569870	6	28	from	homogenous	996:1005	arg1	cereals					1020:1026	the three cereals	1010:1026	the three cereals	1010:1026	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	5	29	theme	Large	758:762	arg1	granules					796:803	Large type-A and small type-B starchy granules	758:803	Large type-A and small type-B starchy granules	758:803	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	9	30	theme	higher	1529:1534	arg1	bio-accessibility					1536:1552	the higher bio-accessibility	1525:1552	the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats	1525:1607	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	6	31	theme	protein	1054:1060	arg1	globoids					1067:1074	the pericarp and protein body globoids	1037:1074	globoids	1067:1074	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	0	32	theme	Novel	0:4	arg1	granules					79:86	starchy granules	71:86	starchy granules of three cereals	71:103	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	0	32	theme	Novel	0:4	arg1	insights					6:13	Novel insights	0:13	Novel insights into pericarp	0:27	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	0	32	theme	Novel	0:4	arg1	globoids					43:50	protein body globoids	30:50	protein body globoids of aleurone layer	30:68	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	7	33	dep	higher	1140:1145	arg1	concentrations					1155:1168	P and K concentrations	1147:1168	P and K concentrations	1147:1168	In the protein body globoids, oats showed higher P and K concentrations than barley and einkorn wheat.					
29569870	4	34	theme	aleurone	673:680	arg1	layer					682:686	The aleurone layer	669:686	The aleurone layer of each cereal type	669:706	The aleurone layer of each cereal type contained protein body globoids within its cells.					
29569870	2	35	theme	morphological	418:430	arg1	structures					432:441	The morphological structures	414:441	The morphological structures	414:441	The morphological structures, chemical elemental composition and surface characteristics of the three cereals were described.					
29569870	7	36	theme	body	1113:1116	arg1	globoids					1118:1125	the protein body globoids	1101:1125	the protein body globoids	1101:1125	In the protein body globoids, oats showed higher P and K concentrations than barley and einkorn wheat.					
29569870	9	37	theme	technological	1666:1678	arg1	standpoints					1680:1690	technological standpoints	1666:1690	technological standpoints	1666:1690	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	0	38	theme	cereals	97:103	arg1	granules					79:86	starchy granules	71:86	starchy granules of three cereals	71:103	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	0	38	theme	cereals	97:103	arg1	insights					6:13	Novel insights	0:13	Novel insights into pericarp	0:27	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	9	39	theme	einkorn	1557:1563	arg1	nutrients					1571:1579	einkorn wheat nutrients	1557:1579	einkorn wheat nutrients	1557:1579	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	9	40	theme	grains	1500:1505	arg1	makeup					1480:1485	the morphological and chemical makeup	1449:1485	the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats	1449:1607	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	5	41	theme	barley	839:844	arg1	endosperm					826:834	the endosperm	822:834	the endosperm of barley and einkorn wheat	822:862	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	0	42	theme	protein	30:36	arg1	insights					6:13	Novel insights	0:13	Novel insights into pericarp	0:27	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	0	42	theme	protein	30:36	arg1	globoids					43:50	protein body globoids	30:50	protein body globoids of aleurone layer	30:68	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	8	43	theme	wheat	1262:1266	arg1	granules					1276:1283	einkorn wheat starchy granules	1254:1283	einkorn wheat starchy granules	1254:1283	Regarding the topographic profiles, detected by AFM, einkorn wheat starchy granules showed a surface profile that differed significantly from that of oats and barley, which were quite similar to one another.					
29569870	0	44	theme	atomic	118:123	arg1	microscopy					131:140	atomic force microscopy	118:140	atomic force microscopy	118:140	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	3	45	theme	nutrients	658:666	arg1	absorption					644:653	absorption	644:653	absorption	644:653	Regarding the morphology, barley showed the thickest pericarp, providing a strong barrier digestion and absorption of nutrients.					
29569870	3	45	theme	nutrients	658:666	arg1	digestion					630:638	a strong barrier digestion	613:638	a strong barrier digestion	613:638	Regarding the morphology, barley showed the thickest pericarp, providing a strong barrier digestion and absorption of nutrients.					
29569870	9	46	theme	wheat	1565:1569	arg1	nutrients					1571:1579	einkorn wheat nutrients	1557:1579	einkorn wheat nutrients	1557:1579	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	5	47	theme	irregular	873:881	arg1	granules					891:898	irregular starchy granules	873:898	irregular starchy granules	873:898	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	8	48	theme	einkorn	1254:1260	arg1	granules					1276:1283	einkorn wheat starchy granules	1254:1283	einkorn wheat starchy granules	1254:1283	Regarding the topographic profiles, detected by AFM, einkorn wheat starchy granules showed a surface profile that differed significantly from that of oats and barley, which were quite similar to one another.					
29569870	5	49	theme	starchy	788:794	arg1	granules					796:803	Large type-A and small type-B starchy granules	758:803	Large type-A and small type-B starchy granules	758:803	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	1	50	theme	Atomic	307:312	arg1	AFM					332:334	AFM	332:334	AFM	332:334	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	50	theme	Atomic	307:312	arg1	Microscopy					320:329	Atomic Force Microscopy	307:329	Atomic Force Microscopy (AFM) analysis	307:344	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	5	51	theme	small	775:779	arg1	starchy					788:794	small type-B starchy	775:794	small type-B starchy	775:794	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	7	52	theme	P	1147:1147	arg1	concentrations					1155:1168	P and K concentrations	1147:1168	P and K concentrations	1147:1168	In the protein body globoids, oats showed higher P and K concentrations than barley and einkorn wheat.					
29569870	5	53	theme	einkorn	850:856	arg1	wheat					858:862	einkorn wheat	850:862	einkorn wheat	850:862	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	2	54	theme	elemental	453:461	arg1	composition					463:473	chemical elemental composition	444:473	chemical elemental composition	444:473	The morphological structures, chemical elemental composition and surface characteristics of the three cereals were described.					
29569870	1	55	theme	Force	314:318	arg1	AFM					332:334	AFM	332:334	AFM	332:334	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	55	theme	Force	314:318	arg1	Microscopy					320:329	Atomic Force Microscopy	307:329	Atomic Force Microscopy (AFM) analysis	307:344	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	3	56	theme	thickest	584:591	arg1	pericarp					593:600	the thickest pericarp	580:600	the thickest pericarp	580:600	Regarding the morphology, barley showed the thickest pericarp, providing a strong barrier digestion and absorption of nutrients.					
29569870	4	57	contain	contained	708:716	arg2	globoids					731:738	protein body globoids	718:738	protein body globoids	718:738	The aleurone layer of each cereal type contained protein body globoids within its cells.					
29569870	4	57	contain	contained	708:716	arg1	layer					682:686	The aleurone layer	669:686	The aleurone layer of each cereal type	669:706	The aleurone layer of each cereal type contained protein body globoids within its cells.					
29569870	4	58	theme	protein	718:724	arg1	globoids					731:738	protein body globoids	718:738	protein body globoids	718:738	The aleurone layer of each cereal type contained protein body globoids within its cells.					
29569870	0	59	theme	body	38:41	arg1	insights					6:13	Novel insights	0:13	Novel insights into pericarp	0:27	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	0	59	theme	body	38:41	arg1	globoids					43:50	protein body globoids	30:50	protein body globoids of aleurone layer	30:68	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	1	60	theme	Microscopy	320:329	arg1	analysis					337:344	Atomic Force Microscopy (AFM) analysis	307:344	Atomic Force Microscopy (AFM) analysis	307:344	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	0	61	theme	force	125:129	arg1	microscopy					131:140	atomic force microscopy	118:140	atomic force microscopy	118:140	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	7	62	theme	einkorn	1186:1192	arg1	wheat					1194:1198	einkorn wheat	1186:1198	einkorn wheat	1186:1198	In the protein body globoids, oats showed higher P and K concentrations than barley and einkorn wheat.					
29569870	6	63	from	cereals	1020:1026	arg1	composition					950:960	The starchy granule elemental composition	920:960	The starchy granule elemental composition	920:960	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	6	63	from	cereals	1020:1026	arg1	homogenous					996:1005	homogenous	996:1005	homogenous	996:1005	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	1	64	dep	caryopses	372:380	arg1	oat					391:393	oat	391:393	oat	391:393	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	64	dep	caryopses	372:380	arg1	wheat					407:411	einkorn wheat	399:411	einkorn wheat	399:411	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	64	dep	caryopses	372:380	arg1	barley					383:388	barley	383:388	barley	383:388	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	64	dep	caryopses	372:380	arg1	caryopses					372:380	three different cereal caryopses	349:380	three different cereal caryopses: barley, oat and einkorn wheat	349:411	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	6	65	theme	starchy	924:930	arg1	composition					950:960	The starchy granule elemental composition	920:960	The starchy granule elemental composition	920:960	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	6	65	theme	starchy	924:930	arg1	homogenous					996:1005	homogenous	996:1005	homogenous	996:1005	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	0	66	theme	environmental	146:158	arg1	microscopy					180:189	environmental scanning electronic microscopy	146:189	environmental scanning electronic microscopy	146:189	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	1	67	theme	einkorn	399:405	arg1	wheat					407:411	einkorn wheat	399:411	einkorn wheat	399:411	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	67	theme	einkorn	399:405	arg1	caryopses					372:380	three different cereal caryopses	349:380	three different cereal caryopses: barley, oat and einkorn wheat	349:411	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	3	68	theme	barrier	622:628	arg1	digestion					630:638	a strong barrier digestion	613:638	a strong barrier digestion	613:638	Regarding the morphology, barley showed the thickest pericarp, providing a strong barrier digestion and absorption of nutrients.					
29569870	9	69	theme	nutrients	1571:1579	arg1	bio-accessibility					1536:1552	the higher bio-accessibility	1525:1552	the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats	1525:1607	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	9	70	theme	chemical	1471:1478	arg1	makeup					1480:1485	the morphological and chemical makeup	1449:1485	the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats	1449:1607	The present work provides insights into the morphological and chemical makeup of the three grains shedding light on the higher bio-accessibility of einkorn wheat nutrients compared to barley and oats, providing important suggestions for human nutrition and technological standpoints.					
29569870	8	71	theme	surface	1294:1300	arg1	profile					1302:1308	a surface profile	1292:1308	a surface profile that differed significantly from that of oats and barley, which were quite similar to one another	1292:1406	Regarding the topographic profiles, detected by AFM, einkorn wheat starchy granules showed a surface profile that differed significantly from that of oats and barley, which were quite similar to one another.					
29569870	4	72	theme	body	726:729	arg1	globoids					731:738	protein body globoids	718:738	protein body globoids	718:738	The aleurone layer of each cereal type contained protein body globoids within its cells.					
29569870	0	73	theme	aleurone	55:62	arg1	layer					64:68	aleurone layer	55:68	aleurone layer	55:68	Novel insights into pericarp, protein body globoids of aleurone layer, starchy granules of three cereals gained using atomic force microscopy and environmental scanning electronic microscopy.					
29569870	5	74	theme	starchy	883:889	arg1	granules					891:898	irregular starchy granules	873:898	irregular starchy granules	873:898	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	8	75	theme	topographic	1215:1225	arg1	profiles					1227:1234	the topographic profiles	1211:1234	the topographic profiles	1211:1234	Regarding the topographic profiles, detected by AFM, einkorn wheat starchy granules showed a surface profile that differed significantly from that of oats and barley, which were quite similar to one another.					
29569870	1	76	theme	Environmental	218:230	arg1	ESEM-EDS					293:300	ESEM-EDS	293:300	ESEM-EDS	293:300	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	76	theme	Environmental	218:230	arg1	Spectroscopy					279:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy	218:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS)	218:301	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	5	77	theme	type-A	764:769	arg1	granules					796:803	Large type-A and small type-B starchy granules	758:803	Large type-A and small type-B starchy granules	758:803	Large type-A and small type-B starchy granules were revealed in the endosperm of barley and einkorn wheat, whereas irregular starchy granules were found in oats.					
29569870	3	78	theme	strong	615:620	arg1	digestion					630:638	a strong barrier digestion	613:638	a strong barrier digestion	613:638	Regarding the morphology, barley showed the thickest pericarp, providing a strong barrier digestion and absorption of nutrients.					
29569870	6	79	theme	elemental	940:948	arg1	composition					950:960	The starchy granule elemental composition	920:960	The starchy granule elemental composition	920:960	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	6	79	theme	elemental	940:948	arg1	homogenous					996:1005	homogenous	996:1005	homogenous	996:1005	The starchy granule elemental composition, detected by ESEM-EDS, was rather homogenous in the three cereals, whereas the pericarp and protein body globoids showed heterogeneity.					
29569870	1	80	theme	Scanning	232:239	arg1	ESEM-EDS					293:300	ESEM-EDS	293:300	ESEM-EDS	293:300	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
29569870	1	80	theme	Scanning	232:239	arg1	Spectroscopy					279:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy	218:290	Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS)	218:301	In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat.					
31349521	8	0	theme	soft	1346:1349	arg1	engineering					1358:1368	soft tissue engineering	1346:1368	soft tissue engineering	1346:1368	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	6	1	theme	Similar	912:918	arg1	reinforcements					920:933	Similar reinforcements	912:933	Similar reinforcements	912:933	Similar reinforcements were observed on compressive modulus, while oscillatory gel strength reinforcement was found to be positively correlated to the filler aspect ratio.					
31349521	8	2	theme	different	1264:1272	arg1	surfaces					1283:1290	different scaffold surfaces	1264:1290	different scaffold surfaces	1264:1290	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	1	3	theme	crystalline	136:146	arg1	structure					148:156	highly crystalline structure	129:156	highly crystalline structure	129:156	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	0	4	theme	functions	73:81	arg1	study					58:62	A comparative study	44:62	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.	0:116	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.					
31349521	4	5	theme	silk	633:636	arg1	fillers					638:644	All silk fillers	629:644	All silk fillers	629:644	All silk fillers showed different degrees of improvement on printability in terms of ink rheology and printing shape fidelity.					
31349521	1	6	theme	functionalized	254:267	arg1	Silk					118:121	Silk	118:121	Silk	118:121	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	1	6	theme	functionalized	254:267	arg1	scaffolds					279:287	build functionalized composite scaffolds	248:287	build functionalized composite scaffolds	248:287	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	0	7	theme	printing	89:96	arg1	scaffolds					107:115	3D printing hydrogel scaffolds	86:115	3D printing hydrogel scaffolds	86:115	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.					
31349521	4	8	theme	rheology	718:725	arg1	terms					705:709	terms	705:709	terms of ink rheology and printing shape fidelity	705:753	All silk fillers showed different degrees of improvement on printability in terms of ink rheology and printing shape fidelity.					
31349521	3	9	theme	fillers	461:467	arg1	forms					447:451	The three forms	437:451	The three forms of silk fillers with varied shapes and dimensions	437:501	The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property.					
31349521	3	10	theme	different	521:529	arg1	methods					542:548	different processing methods	521:548	different processing methods	521:548	The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property.					
31349521	1	11	theme	well-documented	162:176	arg1	biocompatibility					178:193	well-documented biocompatibility	162:193	well-documented biocompatibility	162:193	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	5	12	theme	contact	871:877	arg1	angle					879:883	the contact angle	867:883	the contact angle	867:883	Different silk fillers led to different scaffold surface morphology and different roughness, while all reduced the contact angle compared to pure chitosan.					
31349521	0	13	theme	3D	86:87	arg1	scaffolds					107:115	3D printing hydrogel scaffolds	86:115	3D printing hydrogel scaffolds	86:115	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.					
31349521	1	14	with	Silk	118:121	arg1	structure					148:156	highly crystalline structure	129:156	highly crystalline structure	129:156	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	1	14	with	Silk	118:121	arg1	biocompatibility					178:193	well-documented biocompatibility	162:193	well-documented biocompatibility	162:193	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	7	15	theme	steady	1163:1168	arg1	growth					1175:1180	a steady cell growth	1161:1180	a steady cell growth	1161:1180	Addition of silk introduced no cytotoxicity for that all scaffolds supported a steady cell growth using human fibroblasts.					
31349521	3	16	theme	processing	531:540	arg1	methods					542:548	different processing methods	521:548	different processing methods	521:548	The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property.					
31349521	8	17	theme	Meanwhile	1207:1215	arg1	behaviours					1236:1245	Meanwhile different cellular behaviours	1207:1245	Meanwhile different cellular behaviours	1207:1245	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	8	18	theme	cellular	1227:1234	arg1	behaviours					1236:1245	Meanwhile different cellular behaviours	1207:1245	Meanwhile different cellular behaviours	1207:1245	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	6	19	theme	oscillatory	979:989	arg1	reinforcement					1004:1016	oscillatory gel strength reinforcement	979:1016	oscillatory gel strength reinforcement	979:1016	Similar reinforcements were observed on compressive modulus, while oscillatory gel strength reinforcement was found to be positively correlated to the filler aspect ratio.					
31349521	4	20	theme	fidelity	746:753	arg1	terms					705:709	terms	705:709	terms of ink rheology and printing shape fidelity	705:753	All silk fillers showed different degrees of improvement on printability in terms of ink rheology and printing shape fidelity.					
31349521	4	21	from	degrees	663:669	arg1	printability					689:700	printability	689:700	printability	689:700	All silk fillers showed different degrees of improvement on printability in terms of ink rheology and printing shape fidelity.					
31349521	2	22	theme	silk	381:384	arg1	microfibres					386:396	silk microfibres	381:396	silk microfibres	381:396	In the present study, we developed chitosan/silk composite scaffolds using silk particles, silk microfibres and nanofibres via 3D printing method.					
31349521	0	23	theme	Silk	0:3	arg1	particles					5:13	Silk particles	0:13	Silk particles	0:13	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.					
31349521	1	24	theme	composite	269:277	arg1	Silk					118:121	Silk	118:121	Silk	118:121	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	1	24	theme	composite	269:277	arg1	scaffolds					279:287	build functionalized composite scaffolds	248:287	build functionalized composite scaffolds	248:287	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	4	25	theme	shape	740:744	arg1	fidelity					746:753	printing shape fidelity	731:753	printing shape fidelity	731:753	All silk fillers showed different degrees of improvement on printability in terms of ink rheology and printing shape fidelity.					
31349521	4	26	theme	improvement	674:684	arg1	degrees					663:669	different degrees	653:669	different degrees of improvement on printability	653:700	All silk fillers showed different degrees of improvement on printability in terms of ink rheology and printing shape fidelity.					
31349521	7	27	theme	human	1188:1192	arg1	fibroblasts					1194:1204	human fibroblasts	1188:1204	human fibroblasts	1188:1204	Addition of silk introduced no cytotoxicity for that all scaffolds supported a steady cell growth using human fibroblasts.					
31349521	3	28	theme	thermal	611:617	arg1	property					619:626	thermal property	611:626	thermal property	611:626	The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property.					
31349521	7	29	theme	silk	1096:1099	arg1	Addition					1084:1091	Addition	1084:1091	Addition of silk	1084:1099	Addition of silk introduced no cytotoxicity for that all scaffolds supported a steady cell growth using human fibroblasts.					
31349521	2	30	theme	present	297:303	arg1	study					305:309	the present study	293:309	the present study	293:309	In the present study, we developed chitosan/silk composite scaffolds using silk particles, silk microfibres and nanofibres via 3D printing method.					
31349521	2	31	theme	printing	420:427	arg1	method					429:434	3D printing method	417:434	3D printing method	417:434	In the present study, we developed chitosan/silk composite scaffolds using silk particles, silk microfibres and nanofibres via 3D printing method.					
31349521	6	32	theme	gel	991:993	arg1	reinforcement					1004:1016	oscillatory gel strength reinforcement	979:1016	oscillatory gel strength reinforcement	979:1016	Similar reinforcements were observed on compressive modulus, while oscillatory gel strength reinforcement was found to be positively correlated to the filler aspect ratio.					
31349521	0	33	theme	hydrogel	98:105	arg1	scaffolds					107:115	3D printing hydrogel scaffolds	86:115	3D printing hydrogel scaffolds	86:115	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.					
31349521	2	34	theme	3D	417:418	arg1	method					429:434	3D printing method	417:434	3D printing method	417:434	In the present study, we developed chitosan/silk composite scaffolds using silk particles, silk microfibres and nanofibres via 3D printing method.					
31349521	5	35	theme	different	786:794	arg1	morphology					813:822	different scaffold surface morphology	786:822	different scaffold surface morphology	786:822	Different silk fillers led to different scaffold surface morphology and different roughness, while all reduced the contact angle compared to pure chitosan.					
31349521	4	36	theme	ink	714:716	arg1	rheology					718:725	ink rheology	714:725	ink rheology	714:725	All silk fillers showed different degrees of improvement on printability in terms of ink rheology and printing shape fidelity.					
31349521	6	37	theme	aspect	1070:1075	arg1	ratio					1077:1081	the filler aspect ratio	1059:1081	the filler aspect ratio	1059:1081	Similar reinforcements were observed on compressive modulus, while oscillatory gel strength reinforcement was found to be positively correlated to the filler aspect ratio.					
31349521	4	38	theme	different	653:661	arg1	degrees					663:669	different degrees	653:669	different degrees of improvement on printability	653:700	All silk fillers showed different degrees of improvement on printability in terms of ink rheology and printing shape fidelity.					
31349521	5	39	theme	scaffold	796:803	arg1	morphology					813:822	different scaffold surface morphology	786:822	different scaffold surface morphology	786:822	Different silk fillers led to different scaffold surface morphology and different roughness, while all reduced the contact angle compared to pure chitosan.					
31349521	3	40	with	fillers	461:467	arg1	shapes					481:486	varied shapes	474:486	varied shapes	474:486	The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property.					
31349521	3	40	with	fillers	461:467	arg1	dimensions					492:501	dimensions	492:501	dimensions	492:501	The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property.					
31349521	0	41	from	study	58:62	arg1	scaffolds					107:115	3D printing hydrogel scaffolds	86:115	3D printing hydrogel scaffolds	86:115	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.					
31349521	5	42	theme	Different	756:764	arg1	fillers					771:777	Different silk fillers	756:777	Different silk fillers	756:777	Different silk fillers led to different scaffold surface morphology and different roughness, while all reduced the contact angle compared to pure chitosan.					
31349521	5	43	theme	surface	805:811	arg1	morphology					813:822	different scaffold surface morphology	786:822	different scaffold surface morphology	786:822	Different silk fillers led to different scaffold surface morphology and different roughness, while all reduced the contact angle compared to pure chitosan.					
31349521	6	44	theme	strength	995:1002	arg1	reinforcement					1004:1016	oscillatory gel strength reinforcement	979:1016	oscillatory gel strength reinforcement	979:1016	Similar reinforcements were observed on compressive modulus, while oscillatory gel strength reinforcement was found to be positively correlated to the filler aspect ratio.					
31349521	2	45	theme	composite	339:347	arg1	scaffolds					349:357	chitosan/silk composite scaffolds	325:357	chitosan/silk composite scaffolds	325:357	In the present study, we developed chitosan/silk composite scaffolds using silk particles, silk microfibres and nanofibres via 3D printing method.					
31349521	7	46	theme	cell	1170:1173	arg1	growth					1175:1180	a steady cell growth	1161:1180	a steady cell growth	1161:1180	Addition of silk introduced no cytotoxicity for that all scaffolds supported a steady cell growth using human fibroblasts.					
31349521	3	47	theme	crystalline	585:595	arg1	structure					597:605	crystalline structure	585:605	crystalline structure	585:605	The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property.					
31349521	2	48	theme	chitosan/silk	325:337	arg1	scaffolds					349:357	chitosan/silk composite scaffolds	325:357	chitosan/silk composite scaffolds	325:357	In the present study, we developed chitosan/silk composite scaffolds using silk particles, silk microfibres and nanofibres via 3D printing method.					
31349521	6	49	theme	compressive	952:962	arg1	modulus					964:970	compressive modulus	952:970	compressive modulus	952:970	Similar reinforcements were observed on compressive modulus, while oscillatory gel strength reinforcement was found to be positively correlated to the filler aspect ratio.					
31349521	8	50	located	observed	1252:1259	arg1	surfaces					1283:1290	different scaffold surfaces	1264:1290	different scaffold surfaces	1264:1290	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	8	50	located	observed	1252:1259	arg2	behaviours					1236:1245	Meanwhile different cellular behaviours	1207:1245	Meanwhile different cellular behaviours	1207:1245	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	4	51	theme	printing	731:738	arg1	fidelity					746:753	printing shape fidelity	731:753	printing shape fidelity	731:753	All silk fillers showed different degrees of improvement on printability in terms of ink rheology and printing shape fidelity.					
31349521	8	52	theme	specific	1322:1329	arg1	application					1331:1341	specific application	1322:1341	specific application	1322:1341	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	6	53	located	observed	940:947	arg1	modulus					964:970	compressive modulus	952:970	compressive modulus	952:970	Similar reinforcements were observed on compressive modulus, while oscillatory gel strength reinforcement was found to be positively correlated to the filler aspect ratio.					
31349521	6	53	located	observed	940:947	arg2	reinforcements					920:933	Similar reinforcements	912:933	Similar reinforcements	912:933	Similar reinforcements were observed on compressive modulus, while oscillatory gel strength reinforcement was found to be positively correlated to the filler aspect ratio.					
31349521	1	54	used	used	215:218	arg2	Silk					118:121	Silk	118:121	Silk	118:121	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	1	54	used	used	215:218	arg2	material					235:242	reinforcing material	223:242	reinforcing material	223:242	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	1	54	used	used	215:218	arg2	scaffolds					279:287	build functionalized composite scaffolds	248:287	build functionalized composite scaffolds	248:287	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	8	55	theme	different	1217:1225	arg1	behaviours					1236:1245	Meanwhile different cellular behaviours	1207:1245	Meanwhile different cellular behaviours	1207:1245	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	3	56	theme	varied	474:479	arg1	shapes					481:486	varied shapes	474:486	varied shapes	474:486	The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property.					
31349521	8	57	theme	scaffold	1274:1281	arg1	surfaces					1283:1290	different scaffold surfaces	1264:1290	different scaffold surfaces	1264:1290	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	5	58	theme	silk	766:769	arg1	fillers					771:777	Different silk fillers	756:777	Different silk fillers	756:777	Different silk fillers led to different scaffold surface morphology and different roughness, while all reduced the contact angle compared to pure chitosan.					
31349521	6	59	theme	filler	1063:1068	arg1	ratio					1077:1081	the filler aspect ratio	1059:1081	the filler aspect ratio	1059:1081	Similar reinforcements were observed on compressive modulus, while oscillatory gel strength reinforcement was found to be positively correlated to the filler aspect ratio.					
31349521	0	60	dep	particles	5:13	arg1	study					58:62	A comparative study	44:62	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.	0:116	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.					
31349521	3	61	theme	silk	456:459	arg1	fillers					461:467	silk fillers	456:467	silk fillers with varied shapes and dimensions	456:501	The three forms of silk fillers with varied shapes and dimensions were obtained via different processing methods and evaluated of their morphology, crystalline structure and thermal property.					
31349521	5	62	theme	pure	897:900	arg1	chitosan					902:909	pure chitosan	897:909	pure chitosan	897:909	Different silk fillers led to different scaffold surface morphology and different roughness, while all reduced the contact angle compared to pure chitosan.					
31349521	8	63	theme	tissue	1351:1356	arg1	engineering					1358:1368	soft tissue engineering	1346:1368	soft tissue engineering	1346:1368	Meanwhile different cellular behaviours were observed on different scaffold surfaces, which can possibly intriguer specific application on soft tissue engineering.					
31349521	5	64	theme	different	828:836	arg1	roughness					838:846	different roughness	828:846	different roughness	828:846	Different silk fillers led to different scaffold surface morphology and different roughness, while all reduced the contact angle compared to pure chitosan.					
31349521	0	65	theme	comparative	46:56	arg1	study					58:62	A comparative study	44:62	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.	0:116	Silk particles, microfibres and nanofibres: A comparative study of their functions in 3D printing hydrogel scaffolds.					
31349521	2	66	theme	silk	365:368	arg1	particles					370:378	silk particles	365:378	silk particles	365:378	In the present study, we developed chitosan/silk composite scaffolds using silk particles, silk microfibres and nanofibres via 3D printing method.					
31349521	1	67	theme	reinforcing	223:233	arg1	Silk					118:121	Silk	118:121	Silk	118:121	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
31349521	1	67	theme	reinforcing	223:233	arg1	material					235:242	reinforcing material	223:242	reinforcing material	223:242	Silk, with highly crystalline structure and well-documented biocompatibility, is promising to be used as reinforcing material and build functionalized composite scaffolds.					
30890432	4	0	theme	several	774:780	arg1	genes					796:800	several innate immune genes	774:800	several innate immune genes	774:800	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	9	1	theme	docosahexaenoic	1928:1942	arg1	acid					1944:1947	docosahexaenoic acid	1928:1947	docosahexaenoic acid	1928:1947	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	1	2	theme	ester	241:245	arg1	deposition					247:256	lipid ester deposition	235:256	lipid ester deposition	235:256	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	4	3	theme	immune	789:794	arg1	genes					796:800	several innate immune genes	774:800	several innate immune genes	774:800	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	4	4	theme	TG	530:531	arg1	content					533:539	TG content	530:539	TG content	530:539	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	3	5	from	equivalent	454:463	arg1	vertebrates					485:495	vertebrates	485:495	vertebrates	485:495	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	8	6	theme	lipid	1422:1426	arg1	mix					1428:1430	exogenous lipid mix	1412:1430	exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA	1412:1469	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	6	theme	lipid	1422:1426	arg1	LM					1433:1434	LM	1433:1434	LM; oleic acid [OA	1433:1450	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	7	theme	300 μM	1795:1800	arg1	mix					1818:1820	300 μM exogenous lipid mix	1795:1820	300 μM exogenous lipid mix	1795:1820	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	12	8	theme	polyunsaturated	2478:2492	arg1	acid					2500:2503	long-chain polyunsaturated fatty acid	2467:2503	long-chain polyunsaturated fatty acid	2467:2503	Additionally, long-chain polyunsaturated fatty acid enriched LDs play a unique role in regulating this process.					
30890432	3	9	theme	cells	476:480	arg1	equivalent					454:463	the equivalent	450:463	the equivalent of myeloid cells in vertebrates	450:495	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	3	9	theme	cells	476:480	arg1	cells					433:437	Ctenopharyngodon idella kidney (CIK) cells	396:437	Ctenopharyngodon idella kidney (CIK) cells	396:437	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	1	10	theme	immune	170:175	arg1	modulators					177:186	important immune modulators	160:186	important immune modulators in mammals	160:197	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	1	10	theme	immune	170:175	arg1	droplets					108:115	Lipid droplets	102:115	Lipid droplets (LDs)	102:121	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	7	11	theme	acid	1237:1240	arg1	level					1242:1246	the non-esterified fatty acid level	1212:1246	the non-esterified fatty acid level in the medium	1212:1260	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	8	12	theme	lipid	1812:1816	arg1	mix					1818:1820	300 μM exogenous lipid mix	1795:1820	300 μM exogenous lipid mix	1795:1820	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	7	13	from	level	1242:1246	arg1	medium					1255:1260	the medium	1251:1260	the medium	1251:1260	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	4	14	theme	CIK	572:574	arg1	cells					576:580	the CIK cells	568:580	the CIK cells	568:580	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	8	15	theme	acid	1443:1446	arg1	[OA					1448:1450	oleic acid [OA	1437:1450	LM; oleic acid [OA	1433:1450	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	4	16	dep	acid	690:693	arg1	[I					701:702	Poly [I	696:702	Poly [I: C	696:705	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	8	17	from	increase	1614:1621	arg1	levels					1632:1637	lipid levels	1626:1637	lipid levels	1626:1637	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	12	18	theme	long-chain	2467:2476	arg1	acid					2500:2503	long-chain polyunsaturated fatty acid	2467:2503	long-chain polyunsaturated fatty acid	2467:2503	Additionally, long-chain polyunsaturated fatty acid enriched LDs play a unique role in regulating this process.					
30890432	8	19	theme	24 h.	1570:1574	arg1	size					1579:1582	24 h. LD size	1570:1582	24 h. LD size	1570:1582	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	4	20	from	increases	734:742	arg1	expression					760:769	the relative expression	747:769	the relative expression of several innate immune genes	747:800	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	1	21	theme	additional	203:212	arg1	function					223:230	additional to their function	203:230	additional to their function of lipid ester deposition	203:256	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	12	22	dep	enriched	2505:2512	arg1	acid					2500:2503	long-chain polyunsaturated fatty acid	2467:2503	long-chain polyunsaturated fatty acid	2467:2503	Additionally, long-chain polyunsaturated fatty acid enriched LDs play a unique role in regulating this process.					
30890432	12	23	theme	unique	2525:2530	arg1	role					2532:2535	a unique role	2523:2535	a unique role	2523:2535	Additionally, long-chain polyunsaturated fatty acid enriched LDs play a unique role in regulating this process.					
30890432	8	24	theme	linoleic	1453:1460	arg1	[LA					1467:1469	linoleic acid [LA	1453:1469	exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA	1412:1469	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	5	25	theme	acid	818:821	arg1	compositions					823:834	fatty acid compositions	812:834	fatty acid compositions of the triglycerides	812:855	However, fatty acid compositions of the triglycerides were not changed after treatment with these three pathogenic mimics.					
30890432	12	26	theme	fatty	2494:2498	arg1	acid					2500:2503	long-chain polyunsaturated fatty acid	2467:2503	long-chain polyunsaturated fatty acid	2467:2503	Additionally, long-chain polyunsaturated fatty acid enriched LDs play a unique role in regulating this process.					
30890432	1	27	from	modulators	177:186	arg1	mammals					191:197	mammals	191:197	mammals	191:197	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	2	28	from	role	272:275	arg1	immunity					292:299	fish immunity	287:299	fish immunity	287:299	However, the role of LDs in fish immunity remains poorly understood.					
30890432	7	29	theme	transportation	1141:1154	arg1	genes					1156:1160	lipid transportation genes	1135:1160	lipid transportation genes	1135:1160	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	11	30	from	modulators	2398:2407	arg1	response					2430:2437	the innate immune response	2412:2437	the innate immune response of CIK cells	2412:2450	Overall, the findings of this study demonstrate that LDs are functional organelles that could act as modulators in the innate immune response of CIK cells.					
30890432	9	31	theme	LD	2006:2007	arg1	formation					2009:2017	LD formation	2006:2017	LD formation	2006:2017	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	10	32	theme	immune	2254:2259	arg1	genes					2261:2265	immune genes	2254:2265	immune genes	2254:2265	Finally, atglistatin, an ATGL inhibitor, effectively attenuated the expression of most genes upregulated by ARA or DHA, suggesting that lipolysis may be involved in the regulation of immune genes at the transcriptional level.					
30890432	0	33	theme	idella	81:86	arg1	cells					95:99	Ctenopharyngodon idella kidney cells	64:99	Ctenopharyngodon idella kidney cells	64:99	Lipid droplets participate in modulating innate immune genes in Ctenopharyngodon idella kidney cells.					
30890432	9	34	theme	fatty	1870:1874	arg1	[DHA					1949:1952	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	34	theme	fatty	1870:1874	arg1	acids					1876:1880	different fatty acids	1860:1880	different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM)	1860:1962	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	8	35	theme	lipid	1626:1630	arg1	levels					1632:1637	lipid levels	1626:1637	lipid levels	1626:1637	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	36	theme	highest	1777:1783	arg1	levels					1785:1790	their highest levels	1771:1790	their highest levels	1771:1790	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	10	37	theme	transcriptional	2274:2288	arg1	level					2290:2294	the transcriptional level	2270:2294	the transcriptional level	2270:2294	Finally, atglistatin, an ATGL inhibitor, effectively attenuated the expression of most genes upregulated by ARA or DHA, suggesting that lipolysis may be involved in the regulation of immune genes at the transcriptional level.					
30890432	8	38	dep	LM	1433:1434	arg1	[OA					1448:1450	oleic acid [OA	1437:1450	LM; oleic acid [OA	1433:1450	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	3	39	from	function	347:354	arg1	response					384:391	the innate immune response	366:391	the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates,	366:496	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	8	40	theme	immune	1699:1704	arg1	IRF3					1730:1733	IRF3	1730:1733	IRF3	1730:1733	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	40	theme	immune	1699:1704	arg1	genes					1706:1710	innate immune genes	1692:1710	innate immune genes	1692:1710	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	40	theme	immune	1699:1704	arg1	MyD88					1723:1727	MyD88	1723:1727	MyD88	1723:1727	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	40	theme	immune	1699:1704	arg1	IL-1β					1740:1744	IL-1β	1740:1744	IL-1β	1740:1744	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	41	dep	mix	1428:1430	arg1	[LA					1467:1469	linoleic acid [LA	1453:1469	exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA	1412:1469	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	4	42	theme	polyriboinosinic-polyribocytidylic	655:688	arg1	acid					690:693	polyriboinosinic-polyribocytidylic acid	655:693	polyriboinosinic-polyribocytidylic acid (Poly [I: C])	655:707	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	3	43	theme	immune	377:382	arg1	response					384:391	the innate immune response	366:391	the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates,	366:496	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	0	44	theme	Lipid	0:4	arg1	droplets					6:13	Lipid droplets	0:13	Lipid droplets	0:13	Lipid droplets participate in modulating innate immune genes in Ctenopharyngodon idella kidney cells.					
30890432	5	45	theme	triglycerides	843:855	arg1	compositions					823:834	fatty acid compositions	812:834	fatty acid compositions of the triglycerides	812:855	However, fatty acid compositions of the triglycerides were not changed after treatment with these three pathogenic mimics.					
30890432	6	46	theme	I	946:946	arg1	Poly					940:943	Poly	940:943	Poly (I: C)	940:950	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	6	46	theme	I	946:946	arg1	C					949:949	I: C	946:949	I: C	946:949	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	8	47	theme	immune	1305:1310	arg1	response					1312:1319	the immune response	1301:1319	the immune response	1301:1319	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	11	48	theme	functional	2358:2367	arg1	modulators					2398:2407	modulators	2398:2407	modulators in the innate immune response of CIK cells	2398:2450	Overall, the findings of this study demonstrate that LDs are functional organelles that could act as modulators in the innate immune response of CIK cells.					
30890432	11	48	theme	functional	2358:2367	arg1	organelles					2369:2378	functional organelles	2358:2378	functional organelles that could act as modulators in the innate immune response of CIK cells	2358:2450	Overall, the findings of this study demonstrate that LDs are functional organelles that could act as modulators in the innate immune response of CIK cells.					
30890432	11	48	theme	functional	2358:2367	arg1	LDs					2350:2352	LDs	2350:2352	LDs	2350:2352	Overall, the findings of this study demonstrate that LDs are functional organelles that could act as modulators in the innate immune response of CIK cells.					
30890432	3	49	theme	idella	413:418	arg1	kidney					420:425	Ctenopharyngodon idella kidney	396:425	Ctenopharyngodon idella kidney (CIK) cells	396:437	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	3	49	theme	idella	413:418	arg1	CIK					428:430	CIK	428:430	CIK	428:430	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	8	50	theme	increased	1668:1676	arg1	expression					1678:1687	increased expression	1668:1687	increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix	1668:1820	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	3	51	theme	LDs	359:361	arg1	function					347:354	the function	343:354	the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates,	343:496	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	10	52	theme	genes	2158:2162	arg1	expression					2139:2148	the expression	2135:2148	the expression of most genes upregulated by ARA or DHA	2135:2188	Finally, atglistatin, an ATGL inhibitor, effectively attenuated the expression of most genes upregulated by ARA or DHA, suggesting that lipolysis may be involved in the regulation of immune genes at the transcriptional level.					
30890432	0	53	theme	immune	48:53	arg1	genes					55:59	innate immune genes	41:59	innate immune genes	41:59	Lipid droplets participate in modulating innate immune genes in Ctenopharyngodon idella kidney cells.					
30890432	12	54	theme	enriched	2505:2512	arg1	LDs					2514:2516	long-chain polyunsaturated fatty acid enriched LDs	2467:2516	long-chain polyunsaturated fatty acid enriched LDs	2467:2516	Additionally, long-chain polyunsaturated fatty acid enriched LDs play a unique role in regulating this process.					
30890432	9	55	theme	acid	1912:1915	arg1	[ARA					1917:1920	arachidonic acid [ARA]	1900:1921	arachidonic acid [ARA]	1900:1921	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	11	56	theme	innate	2416:2421	arg1	response					2430:2437	the innate immune response	2412:2437	the innate immune response of CIK cells	2412:2450	Overall, the findings of this study demonstrate that LDs are functional organelles that could act as modulators in the innate immune response of CIK cells.					
30890432	6	57	theme	relative	970:977	arg1	expressions					979:989	the relative expressions	966:989	the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes	966:1074	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	8	58	theme	different	1352:1360	arg1	500 μM					1401:1406	500 μM	1401:1406	500 μM	1401:1406	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	58	theme	different	1352:1360	arg1	concentrations					1362:1375	different concentrations	1352:1375	different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1)	1352:1500	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	9	59	theme	LNA	1895:1897	arg1	[DHA					1949:1952	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	59	theme	LNA	1895:1897	arg1	acids					1876:1880	different fatty acids	1860:1880	different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM)	1860:1962	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	6	60	dep	lipogenic	994:1002	arg1	CPT-1					1063:1067	CPT-1	1063:1067	CPT-1	1063:1067	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	6	60	dep	lipogenic	994:1002	arg1	DGAT					1019:1022	DGAT	1019:1022	DGAT	1019:1022	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	6	60	dep	lipogenic	994:1002	arg1	PPARα					1046:1050	PPARα	1046:1050	PPARα	1046:1050	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	6	60	dep	lipogenic	994:1002	arg1	ATGL					1053:1056	ATGL	1053:1056	ATGL	1053:1056	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	6	60	dep	lipogenic	994:1002	arg1	FAS					1005:1007	FAS	1005:1007	FAS	1005:1007	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	6	60	dep	lipogenic	994:1002	arg1	SCD					1010:1012	SCD	1010:1012	SCD	1010:1012	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	10	61	from	level	2290:2294	arg1	regulation					2240:2249	the regulation	2236:2249	the regulation of immune genes at the transcriptional level	2236:2294	Finally, atglistatin, an ATGL inhibitor, effectively attenuated the expression of most genes upregulated by ARA or DHA, suggesting that lipolysis may be involved in the regulation of immune genes at the transcriptional level.					
30890432	1	62	theme	lipid	235:239	arg1	deposition					247:256	lipid ester deposition	235:256	lipid ester deposition	235:256	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	4	63	theme	LD	516:517	arg1	number					519:524	LD number	516:524	LD number	516:524	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	9	64	theme	acid	1944:1947	arg1	[DHA					1949:1952	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	64	theme	acid	1944:1947	arg1	acids					1876:1880	different fatty acids	1860:1880	different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM)	1860:1962	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	1	65	theme	deposition	247:256	arg1	function					223:230	additional to their function	203:230	additional to their function of lipid ester deposition	203:256	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	4	66	theme	innate	782:787	arg1	genes					796:800	several innate immune genes	774:800	several innate immune genes	774:800	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	7	67	theme	non-esterified	1216:1229	arg1	level					1242:1246	the non-esterified fatty acid level	1212:1246	the non-esterified fatty acid level in the medium	1212:1260	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	6	68	theme	genes	1070:1074	arg1	expressions					979:989	the relative expressions	966:989	the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes	966:1074	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	3	69	theme	myeloid	468:474	arg1	cells					476:480	myeloid cells	468:480	myeloid cells	468:480	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	8	70	theme	exogenous	1412:1420	arg1	mix					1428:1430	exogenous lipid mix	1412:1430	exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA	1412:1469	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	70	theme	exogenous	1412:1420	arg1	LM					1433:1434	LM	1433:1434	LM; oleic acid [OA	1433:1450	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	1	71	theme	important	160:168	arg1	modulators					177:186	important immune modulators	160:186	important immune modulators in mammals	160:197	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	1	71	theme	important	160:168	arg1	droplets					108:115	Lipid droplets	102:115	Lipid droplets (LDs)	102:121	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	7	72	theme	fatty	1231:1235	arg1	level					1242:1246	the non-esterified fatty acid level	1212:1246	the non-esterified fatty acid level in the medium	1212:1260	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	2	73	theme	fish	287:290	arg1	immunity					292:299	fish immunity	287:299	fish immunity	287:299	However, the role of LDs in fish immunity remains poorly understood.					
30890432	8	74	theme	mix	1428:1430	arg1	500 μM					1401:1406	500 μM	1401:1406	500 μM	1401:1406	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	74	theme	mix	1428:1430	arg1	concentrations					1362:1375	different concentrations	1352:1375	different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1)	1352:1500	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	4	75	theme	relative	751:758	arg1	expression					760:769	the relative expression	747:769	the relative expression of several innate immune genes	747:800	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	8	76	theme	exogenous	1802:1810	arg1	mix					1818:1820	300 μM exogenous lipid mix	1795:1820	300 μM exogenous lipid mix	1795:1820	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	2	77	theme	LDs	280:282	arg1	role					272:275	the role	268:275	the role of LDs in fish immunity	268:299	However, the role of LDs in fish immunity remains poorly understood.					
30890432	1	78	dep	function	223:230	arg1	to					214:215	to	214:215	to	214:215	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	5	79	with	treatment	880:888	arg1	mimics					918:923	these three pathogenic mimics	895:923	these three pathogenic mimics	895:923	However, fatty acid compositions of the triglycerides were not changed after treatment with these three pathogenic mimics.					
30890432	8	80	theme	LD	1576:1577	arg1	size					1579:1582	24 h. LD size	1570:1582	24 h. LD size	1570:1582	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	81	theme	oleic	1437:1441	arg1	[OA					1448:1450	oleic acid [OA	1437:1450	LM; oleic acid [OA	1433:1450	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	5	82	theme	fatty	812:816	arg1	compositions					823:834	fatty acid compositions	812:834	fatty acid compositions of the triglycerides	812:855	However, fatty acid compositions of the triglycerides were not changed after treatment with these three pathogenic mimics.					
30890432	4	83	theme	genes	796:800	arg1	expression					760:769	the relative expression	747:769	the relative expression of several innate immune genes	747:800	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	8	84	dep	500 μM	1401:1406	arg1	400					1396:1398	400	1396:1398	400	1396:1398	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	85	theme	lipid-free	1534:1543	arg1	medium					1545:1550	a lipid-free medium	1532:1550	a lipid-free medium	1532:1550	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	4	86	dep	increased	555:563	arg1	accompanied					719:729	accompanied	719:729	accompanied by increases in the relative expression of several innate immune genes	719:800	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	6	87	theme	catabolic	1035:1043	arg1	genes					1070:1074	lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes	994:1074	lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes	994:1074	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	0	88	theme	Ctenopharyngodon	64:79	arg1	idella					81:86	Ctenopharyngodon idella	64:86	Ctenopharyngodon idella kidney cells	64:99	Lipid droplets participate in modulating innate immune genes in Ctenopharyngodon idella kidney cells.					
30890432	8	89	theme	acid	1462:1465	arg1	[LA					1467:1469	linoleic acid [LA	1453:1469	exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA	1412:1469	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	10	90	theme	genes	2261:2265	arg1	regulation					2240:2249	the regulation	2236:2249	the regulation of immune genes at the transcriptional level	2236:2294	Finally, atglistatin, an ATGL inhibitor, effectively attenuated the expression of most genes upregulated by ARA or DHA, suggesting that lipolysis may be involved in the regulation of immune genes at the transcriptional level.					
30890432	7	91	theme	genes	1156:1160	arg1	levels					1125:1130	the mRNA levels	1116:1130	the mRNA levels of lipid transportation genes	1116:1160	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	0	92	theme	kidney	88:93	arg1	cells					95:99	Ctenopharyngodon idella kidney cells	64:99	Ctenopharyngodon idella kidney cells	64:99	Lipid droplets participate in modulating innate immune genes in Ctenopharyngodon idella kidney cells.					
30890432	9	93	theme	LM	1883:1884	arg1	[DHA					1949:1952	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	93	theme	LM	1883:1884	arg1	acids					1876:1880	different fatty acids	1860:1880	different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM)	1860:1962	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	11	94	theme	cells	2446:2450	arg1	response					2430:2437	the innate immune response	2412:2437	the innate immune response of CIK cells	2412:2450	Overall, the findings of this study demonstrate that LDs are functional organelles that could act as modulators in the innate immune response of CIK cells.					
30890432	4	95	dep	[I	701:702	arg1	C					705:705	C	705:705	Poly [I: C	696:705	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	9	96	theme	immune	2030:2035	arg1	expression					2042:2051	innate immune gene expression	2023:2051	innate immune gene expression	2023:2051	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	11	97	theme	CIK	2442:2444	arg1	cells					2446:2450	CIK cells	2442:2450	CIK cells	2442:2450	Overall, the findings of this study demonstrate that LDs are functional organelles that could act as modulators in the innate immune response of CIK cells.					
30890432	9	98	theme	different	1860:1868	arg1	[DHA					1949:1952	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	98	theme	different	1860:1868	arg1	acids					1876:1880	different fatty acids	1860:1880	different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM)	1860:1962	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	99	theme	innate	2023:2028	arg1	expression					2042:2051	innate immune gene expression	2023:2051	innate immune gene expression	2023:2051	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	8	100	theme	linolenic	1472:1480	arg1	acid					1482:1485	linolenic acid	1472:1485	different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1)	1352:1500	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	101	theme	LDs	1294:1296	arg1	role					1286:1289	the role	1282:1289	the role of LDs in the immune response	1282:1319	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	4	102	theme	Poly	696:699	arg1	[I					701:702	Poly [I	696:702	Poly [I: C	696:705	LD number and TG content significantly increased in the CIK cells following exposure to lipopolysaccharide (LPS), peptidoglycan (PGN), and polyriboinosinic-polyribocytidylic acid (Poly [I: C]) for 24 h, accompanied by increases in the relative expression of several innate immune genes.					
30890432	9	103	theme	[ARA	1917:1920	arg1	[DHA					1949:1952	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	103	theme	[ARA	1917:1920	arg1	acids					1876:1880	different fatty acids	1860:1880	different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM)	1860:1962	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	104	theme	gene	2037:2040	arg1	expression					2042:2051	innate immune gene expression	2023:2051	innate immune gene expression	2023:2051	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	8	105	theme	genes	1706:1710	arg1	expression					1678:1687	increased expression	1668:1687	increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix	1668:1820	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	10	106	theme	ATGL	2096:2099	arg1	atglistatin					2080:2090	atglistatin	2080:2090	atglistatin	2080:2090	Finally, atglistatin, an ATGL inhibitor, effectively attenuated the expression of most genes upregulated by ARA or DHA, suggesting that lipolysis may be involved in the regulation of immune genes at the transcriptional level.					
30890432	10	106	theme	ATGL	2096:2099	arg1	inhibitor					2101:2109	an ATGL inhibitor	2093:2109	an ATGL inhibitor	2093:2109	Finally, atglistatin, an ATGL inhibitor, effectively attenuated the expression of most genes upregulated by ARA or DHA, suggesting that lipolysis may be involved in the regulation of immune genes at the transcriptional level.					
30890432	3	107	theme	Ctenopharyngodon	396:411	arg1	idella					413:418	Ctenopharyngodon idella	396:418	Ctenopharyngodon idella kidney (CIK) cells	396:437	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	8	108	from	role	1286:1289	arg1	response					1312:1319	the immune response	1301:1319	the immune response	1301:1319	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	7	109	theme	mRNA	1120:1123	arg1	levels					1125:1130	the mRNA levels	1116:1130	the mRNA levels of lipid transportation genes	1116:1160	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	9	110	theme	OA	1887:1888	arg1	[DHA					1949:1952	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	110	theme	OA	1887:1888	arg1	acids					1876:1880	different fatty acids	1860:1880	different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM)	1860:1962	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	7	111	dep	increase	1107:1114	arg1	ACSL1					1174:1178	ACSL1	1174:1178	ACSL1	1174:1178	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	7	111	dep	increase	1107:1114	arg1	ACSL4					1185:1189	ACSL4	1185:1189	ACSL4	1185:1189	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	7	111	dep	increase	1107:1114	arg1	FATP/CD36					1163:1171	FATP/CD36	1163:1171	FATP/CD36	1163:1171	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
30890432	9	112	theme	CIK	2060:2062	arg1	cells					2064:2068	the CIK cells	2056:2068	the CIK cells	2056:2068	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	1	113	theme	Lipid	102:106	arg1	modulators					177:186	important immune modulators	160:186	important immune modulators in mammals	160:197	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	1	113	theme	Lipid	102:106	arg1	LDs					118:120	LDs	118:120	LDs	118:120	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	1	113	theme	Lipid	102:106	arg1	droplets					108:115	Lipid droplets	102:115	Lipid droplets (LDs)	102:121	Lipid droplets (LDs) are increasingly being recognized as important immune modulators in mammals, in additional to their function of lipid ester deposition.					
30890432	8	114	theme	CIK	1322:1324	arg1	cells					1326:1330	CIK cells	1322:1330	CIK cells	1322:1330	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	9	115	theme	LA	1891:1892	arg1	[DHA					1949:1952	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM	1883:1961	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	9	115	theme	LA	1891:1892	arg1	acids					1876:1880	different fatty acids	1860:1880	different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM)	1860:1962	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	0	116	theme	innate	41:46	arg1	genes					55:59	innate immune genes	41:59	innate immune genes	41:59	Lipid droplets participate in modulating innate immune genes in Ctenopharyngodon idella kidney cells.					
30890432	3	117	theme	innate	370:375	arg1	response					384:391	the innate immune response	366:391	the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates,	366:496	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	3	118	theme	kidney	420:425	arg1	equivalent					454:463	the equivalent	450:463	the equivalent of myeloid cells in vertebrates	450:495	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	3	118	theme	kidney	420:425	arg1	cells					433:437	Ctenopharyngodon idella kidney (CIK) cells	396:437	Ctenopharyngodon idella kidney (CIK) cells	396:437	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	8	119	theme	innate	1692:1697	arg1	IRF3					1730:1733	IRF3	1730:1733	IRF3	1730:1733	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	119	theme	innate	1692:1697	arg1	genes					1706:1710	innate immune genes	1692:1710	innate immune genes	1692:1710	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	119	theme	innate	1692:1697	arg1	MyD88					1723:1727	MyD88	1723:1727	MyD88	1723:1727	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	8	119	theme	innate	1692:1697	arg1	IL-1β					1740:1744	IL-1β	1740:1744	IL-1β	1740:1744	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	11	120	theme	immune	2423:2428	arg1	response					2430:2437	the innate immune response	2412:2437	the innate immune response of CIK cells	2412:2450	Overall, the findings of this study demonstrate that LDs are functional organelles that could act as modulators in the innate immune response of CIK cells.					
30890432	8	121	dep	concentrations	1362:1375	arg1	acid					1482:1485	linolenic acid	1472:1485	different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1)	1352:1500	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	6	122	theme	lipogenic	994:1002	arg1	genes					1070:1074	lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes	994:1074	lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes	994:1074	LPS, PGN, and Poly (I: C) did not alter the relative expressions of lipogenic (FAS, SCD, and DGAT) and lipid catabolic (PPARα, ATGL, and CPT-1) genes.					
30890432	10	123	theme	most	2153:2156	arg1	genes					2158:2162	most genes	2153:2162	most genes upregulated by ARA or DHA	2153:2188	Finally, atglistatin, an ATGL inhibitor, effectively attenuated the expression of most genes upregulated by ARA or DHA, suggesting that lipolysis may be involved in the regulation of immune genes at the transcriptional level.					
30890432	9	124	theme	arachidonic	1900:1910	arg1	[ARA					1917:1920	arachidonic acid [ARA]	1900:1921	arachidonic acid [ARA]	1900:1921	Interestingly, after incubating with different fatty acids (LM, OA, LA, LNA, arachidonic acid [ARA], and docosahexaenoic acid [DHA]; 300 μM), ARA and DHA were more potent in inducing LD formation and innate immune gene expression in the CIK cells.					
30890432	8	125	theme	concentrations	1362:1375	arg1	[LNA					1487:1490	different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1)	1352:1500	different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1)	1352:1500	To further explore the role of LDs in the immune response, CIK cells were incubated with different concentrations (0, 100, 200, 300, 400, 500 μM) of exogenous lipid mix (LM; oleic acid [OA]:linoleic acid [LA]:linolenic acid [LNA] = 2:1:1), and were then transferred to a lipid-free medium and incubated for 24 h. LD size and number increased with the increase in lipid levels, and this was accompanied by increased expression of innate immune genes, including MyD88, IRF3, and IL-1β, which were expressed at their highest levels in 300 μM exogenous lipid mix.					
30890432	11	126	theme	study	2327:2331	arg1	findings					2310:2317	the findings	2306:2317	the findings of this study	2306:2331	Overall, the findings of this study demonstrate that LDs are functional organelles that could act as modulators in the innate immune response of CIK cells.					
30890432	3	127	theme	cells	433:437	arg1	response					384:391	the innate immune response	366:391	the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates,	366:496	In this study, the function of LDs in the innate immune response of Ctenopharyngodon idella kidney (CIK) cells, which are the equivalent of myeloid cells in vertebrates, was investigated.					
30890432	5	128	theme	pathogenic	907:916	arg1	mimics					918:923	these three pathogenic mimics	895:923	these three pathogenic mimics	895:923	However, fatty acid compositions of the triglycerides were not changed after treatment with these three pathogenic mimics.					
30890432	7	129	theme	lipid	1135:1139	arg1	genes					1156:1160	lipid transportation genes	1135:1160	lipid transportation genes	1135:1160	However, these treatments did increase the mRNA levels of lipid transportation genes (FATP/CD36, ACSL1, and ACSL4), and also decreased the non-esterified fatty acid level in the medium.					
29303693	9	0	theme	phenotypic	1202:1211	arg1	data					1213:1216	the genotypic and phenotypic data	1184:1216	the genotypic and phenotypic data	1184:1216	A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp.					
29303693	4	1	dep	3-OH	698:701	arg1	iso-C15:0					718:726	iso-C15:0	718:726	iso-C15:0	718:726	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	1	dep	3-OH	698:701	arg1	C15 					704:707	C15 	704:707	C15 	704:707	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	1	dep	3-OH	698:701	arg1	C15 					742:745	C15 	742:745	C15 	742:745	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	8	2	theme	type	1030:1033	arg1	strains					1035:1041	the type strains	1026:1041	the type strains of recognized species of the genus Polaribacter	1026:1089	The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics.					
29303693	5	3	theme	major	807:811	arg1	lipids					813:818	the major lipids phosphatidylethanolamine	803:843	the major lipids phosphatidylethanolamine	803:843	The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids.					
29303693	9	4	theme	name	1320:1323	arg1	sp					1345:1346	the name Polaribacterstaleyi sp	1316:1346	the name Polaribacterstaleyi sp	1316:1346	A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp.					
29303693	5	5	theme	unidentified	884:895	arg1	lipids					897:902	four unidentified lipids	879:902	four unidentified lipids	879:902	The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids.					
29303693	3	6	theme	highest	501:507	arg1	similarity					518:527	highest sequence similarity	501:527	highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species	501:631	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	11	7	theme	52773T=KMM	1404:1413	arg1	6729T					1415:1419	=KCTC 52773T=KMM 6729T	1398:1419	=KCTC 52773T=KMM 6729T	1398:1419	The type strain is 10Alg 139T (=KCTC 52773T=KMM 6729T).					
29303693	11	7	theme	52773T=KMM	1404:1413	arg1	139T					1392:1395	10Alg 139T	1386:1395	10Alg 139T (=KCTC 52773T=KMM 6729T)	1386:1420	The type strain is 10Alg 139T (=KCTC 52773T=KMM 6729T).					
29303693	9	8	theme	Polaribacterstaleyi	1325:1343	arg1	sp					1345:1346	the name Polaribacterstaleyi sp	1316:1346	the name Polaribacterstaleyi sp	1316:1346	A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp.					
29303693	8	9	theme	genus	1072:1076	arg1	Polaribacter					1078:1089	the genus Polaribacter	1068:1089	the genus Polaribacter	1068:1089	The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics.					
29303693	3	10	theme	Polaribacter	612:623	arg1	species					625:631	other recognized Polaribacter species	595:631	other recognized Polaribacter species	595:631	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	1	11	theme	marine	58:63	arg1	bacterium					65:73	a polysaccharide-degrading marine bacterium	31:73	a polysaccharide-degrading marine bacterium	31:73	nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis.					
29303693	1	11	theme	marine	58:63	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis.					
29303693	4	12	theme	 0	709:710	arg1	3-OH					712:715	 0 3-OH	709:715	 0 3-OH	709:715	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	2	13	theme	motile	161:166	arg1	bacterium					200:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	3	14	theme	KMM	557:559	arg1	3938T					561:565	Polaribacter butkevichii KMM 3938T	532:565	Polaribacter butkevichii KMM 3938T (99.3 %)	532:574	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	14	theme	KMM	557:559	arg1	%					573:573	99.3 %	568:573	99.3 %	568:573	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	2	15	theme	Ahnfeltiato	280:290	arg1	buchiensis					292:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	5	16	theme	unidentified	850:861	arg1	aminolipids					863:873	two unidentified aminolipids	846:873	two unidentified aminolipids	846:873	The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids.					
29303693	3	17	theme	gene	348:351	arg1	sequences					353:361	16S rRNA gene sequences	339:361	16S rRNA gene sequences	339:361	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	7	18	theme	DNA	957:959	arg1	G+C content					961:971	The DNA G+C content	953:971	The DNA G+C content of the type strain	953:990	The DNA G+C content of the type strain is 31.8 mol%.					
29303693	7	18	theme	DNA	957:959	arg1	%					1003:1003	31.8 mol%	995:1003	31.8 mol%	995:1003	The DNA G+C content of the type strain is 31.8 mol%.					
29303693	2	19	theme	alga	275:278	arg1	buchiensis					292:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	5	20	theme	polar	770:774	arg1	profile					782:788	The polar lipid profile	766:788	The polar lipid profile	766:788	The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids.					
29303693	6	21	theme	respiratory	914:924	arg1	menaquinone					938:948	menaquinone 6	938:950	menaquinone 6	938:950	The main respiratory quinone was menaquinone 6.					
29303693	6	21	theme	respiratory	914:924	arg1	quinone					926:932	The main respiratory quinone	905:932	The main respiratory quinone	905:932	The main respiratory quinone was menaquinone 6.					
29303693	2	22	theme	rod-shaped	149:158	arg1	bacterium					200:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	2	23	theme	red	271:273	arg1	buchiensis					292:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	4	24	theme	fatty	648:652	arg1	acids					654:658	The prevalent fatty acids	634:658	The prevalent fatty acids of strain 10Alg 139T	634:679	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	24	theme	fatty	648:652	arg1	iso-C15 					686:693	iso-C15 	686:693	iso-C15 	686:693	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	24	theme	fatty	648:652	arg1	3-OH					698:701	 0 3-OH	695:701	 0 3-OH	695:701	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	8	25	theme	species	1057:1063	arg1	isolate					1014:1020	The new isolate	1006:1020	The new isolate	1006:1020	The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics.					
29303693	8	25	theme	species	1057:1063	arg1	strains					1035:1041	the type strains	1026:1041	the type strains of recognized species of the genus Polaribacter	1026:1089	The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics.					
29303693	8	26	theme	recognized	1046:1055	arg1	species					1057:1063	recognized species	1046:1063	recognized species of the genus Polaribacter	1046:1089	The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics.					
29303693	7	27	theme	type	980:983	arg1	strain					985:990	the type strain	976:990	the type strain	976:990	The DNA G+C content of the type strain is 31.8 mol%.					
29303693	4	28	theme	strain	663:668	arg1	139T					676:679	strain 10Alg 139T	663:679	strain 10Alg 139T	663:679	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	0	29	theme	Polaribacter	0:11	arg1	staleyi					13:19	Polaribacter staleyi	0:19	Polaribacter staleyi	0:19	Polaribacter staleyi sp.					
29303693	6	30	theme	main	909:912	arg1	menaquinone					938:948	menaquinone 6	938:950	menaquinone 6	938:950	The main respiratory quinone was menaquinone 6.					
29303693	6	30	theme	main	909:912	arg1	quinone					926:932	The main respiratory quinone	905:932	The main respiratory quinone	905:932	The main respiratory quinone was menaquinone 6.					
29303693	1	31	theme	red	93:95	arg1	tobuchiensis					112:123	the red alga Ahnfeltia tobuchiensis	89:123	the red alga Ahnfeltia tobuchiensis	89:123	nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis.					
29303693	8	32	theme	Polaribacter	1078:1089	arg1	species					1057:1063	recognized species	1046:1063	recognized species of the genus Polaribacter	1046:1089	The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics.					
29303693	7	33	theme	31.8 mol	995:1002	arg1	G+C content					961:971	The DNA G+C content	953:971	The DNA G+C content of the type strain	953:990	The DNA G+C content of the type strain is 31.8 mol%.					
29303693	7	33	theme	31.8 mol	995:1002	arg1	%					1003:1003	31.8 mol%	995:1003	31.8 mol%	995:1003	The DNA G+C content of the type strain is 31.8 mol%.					
29303693	1	34	theme	alga	97:100	arg1	tobuchiensis					112:123	the red alga Ahnfeltia tobuchiensis	89:123	the red alga Ahnfeltia tobuchiensis	89:123	nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis.					
29303693	9	35	theme	Polaribacter	1292:1303	arg1	species					1271:1277	a novel species	1263:1277	a novel species	1263:1277	A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp.					
29303693	3	36	theme	Flavobacteriaceae	451:467	arg1	member					430:435	a member	428:435	a member of the family Flavobacteriaceae	428:467	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	36	theme	Flavobacteriaceae	451:467	arg1	Bacteroidetes					481:493	the phylum Bacteroidetes	470:493	the phylum Bacteroidetes	470:493	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	36	theme	Flavobacteriaceae	451:467	arg1	Polaribacter					414:425	the genus Polaribacter	404:425	the genus Polaribacter	404:425	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	37	theme	novel	379:383	arg1	strain					385:390	the novel strain	375:390	the novel strain	375:390	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	9	38	theme	novel	1265:1269	arg1	species					1271:1277	a novel species	1263:1277	a novel species	1263:1277	A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp.					
29303693	9	39	from	alga	1247:1250	arg1	isolate					1234:1240	the isolate	1230:1240	the isolate from alga	1230:1250	A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp.					
29303693	2	40	theme	yellow-pigmented	183:198	arg1	bacterium					200:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	1	41	theme	Ahnfeltia	102:110	arg1	tobuchiensis					112:123	the red alga Ahnfeltia tobuchiensis	89:123	the red alga Ahnfeltia tobuchiensis	89:123	nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis.					
29303693	4	42	theme	139T	676:679	arg1	acids					654:658	The prevalent fatty acids	634:658	The prevalent fatty acids of strain 10Alg 139T	634:679	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	42	theme	139T	676:679	arg1	iso-C15 					686:693	iso-C15 	686:693	iso-C15 	686:693	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	42	theme	139T	676:679	arg1	3-OH					698:701	 0 3-OH	695:701	 0 3-OH	695:701	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	3	43	theme	genus	408:412	arg1	member					430:435	a member	428:435	a member of the family Flavobacteriaceae	428:467	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	43	theme	genus	408:412	arg1	Polaribacter					414:425	the genus Polaribacter	404:425	the genus Polaribacter	404:425	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	7	44	theme	strain	985:990	arg1	G+C content					961:971	The DNA G+C content	953:971	The DNA G+C content of the type strain	953:990	The DNA G+C content of the type strain is 31.8 mol%.					
29303693	7	44	theme	strain	985:990	arg1	%					1003:1003	31.8 mol%	995:1003	31.8 mol%	995:1003	The DNA G+C content of the type strain is 31.8 mol%.					
29303693	11	45	theme	type	1371:1374	arg1	strain					1376:1381	The type strain	1367:1381	The type strain	1367:1381	The type strain is 10Alg 139T (=KCTC 52773T=KMM 6729T).					
29303693	11	45	theme	type	1371:1374	arg1	139T					1392:1395	10Alg 139T	1386:1395	10Alg 139T (=KCTC 52773T=KMM 6729T)	1386:1420	The type strain is 10Alg 139T (=KCTC 52773T=KMM 6729T).					
29303693	4	46	theme	prevalent	638:646	arg1	acids					654:658	The prevalent fatty acids	634:658	The prevalent fatty acids of strain 10Alg 139T	634:679	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	46	theme	prevalent	638:646	arg1	iso-C15 					686:693	iso-C15 	686:693	iso-C15 	686:693	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	46	theme	prevalent	638:646	arg1	3-OH					698:701	 0 3-OH	695:701	 0 3-OH	695:701	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	8	47	theme	characteristics	1150:1164	arg1	number					1129:1134	a number	1127:1134	a number of phenotypic characteristics	1127:1164	The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics.					
29303693	8	48	theme	new	1010:1012	arg1	isolate					1014:1020	The new isolate	1006:1020	The new isolate	1006:1020	The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics.					
29303693	3	49	theme	family	444:449	arg1	Flavobacteriaceae					451:467	the family Flavobacteriaceae	440:467	the family Flavobacteriaceae	440:467	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	8	50	theme	phenotypic	1139:1148	arg1	characteristics					1150:1164	phenotypic characteristics	1139:1164	phenotypic characteristics	1139:1164	The new isolate and the type strains of recognized species of the genus Polaribacter were readily distinguished based on a number of phenotypic characteristics.					
29303693	3	51	theme	phylogenetic	308:319	arg1	analysis					321:328	The phylogenetic analysis	304:328	The phylogenetic analysis based on 16S rRNA gene sequences	304:361	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	5	52	dep	lipids	813:818	arg1	phosphatidylethanolamine					820:843	phosphatidylethanolamine	820:843	the major lipids phosphatidylethanolamine	803:843	The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids.					
29303693	9	53	theme	genus	1286:1290	arg1	Polaribacter					1292:1303	the genus Polaribacter	1282:1303	the genus Polaribacter	1282:1303	A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp.					
29303693	2	54	theme	Gram-stain-negative	128:146	arg1	bacterium					200:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	2	55	theme	strain	222:227	arg1	139T					235:238	strain 10Alg 139T	222:238	strain 10Alg 139T	222:238	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	2	56	theme	10Alg	229:233	arg1	139T					235:238	strain 10Alg 139T	222:238	strain 10Alg 139T	222:238	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	9	57	theme	data	1213:1216	arg1	combination					1169:1179	A combination	1167:1179	A combination of the genotypic and phenotypic data	1167:1216	A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp.					
29303693	3	58	theme	other	595:599	arg1	species					625:631	other recognized Polaribacter species	595:631	other recognized Polaribacter species	595:631	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	59	with	member	430:435	arg1	similarity					518:527	highest sequence similarity	501:527	highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species	501:631	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	60	theme	recognized	601:610	arg1	species					625:631	other recognized Polaribacter species	595:631	other recognized Polaribacter species	595:631	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	2	61	theme	Pacific	263:269	arg1	buchiensis					292:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	11	62	theme	=KCTC	1398:1402	arg1	6729T					1415:1419	=KCTC 52773T=KMM 6729T	1398:1419	=KCTC 52773T=KMM 6729T	1398:1419	The type strain is 10Alg 139T (=KCTC 52773T=KMM 6729T).					
29303693	11	62	theme	=KCTC	1398:1402	arg1	139T					1392:1395	10Alg 139T	1386:1395	10Alg 139T (=KCTC 52773T=KMM 6729T)	1386:1420	The type strain is 10Alg 139T (=KCTC 52773T=KMM 6729T).					
29303693	3	63	theme	Polaribacter	532:543	arg1	3938T					561:565	Polaribacter butkevichii KMM 3938T	532:565	Polaribacter butkevichii KMM 3938T (99.3 %)	532:574	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	63	theme	Polaribacter	532:543	arg1	%					573:573	99.3 %	568:573	99.3 %	568:573	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	9	64	theme	genotypic	1188:1196	arg1	data					1213:1216	the genotypic and phenotypic data	1184:1216	the genotypic and phenotypic data	1184:1216	A combination of the genotypic and phenotypic data showed that the isolate from alga represents a novel species of the genus Polaribacter, for which the name Polaribacterstaleyi sp.					
29303693	0	65	dep	sp	21:22	arg1	staleyi					13:19	Polaribacter staleyi	0:19	Polaribacter staleyi	0:19	Polaribacter staleyi sp.					
29303693	4	66	dep	C15 	704:707	arg1	 0					747:748	 0	747:748	 0	747:748	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	66	dep	C15 	704:707	arg1	C15 					754:757	C15 	754:757	C15 	754:757	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	66	dep	C15 	704:707	arg1	3-OH					712:715	 0 3-OH	709:715	 0 3-OH	709:715	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	66	dep	C15 	704:707	arg1	 1ω6c					759:763	 1ω6c	759:763	 1ω6c	759:763	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	66	dep	C15 	704:707	arg1	 0					738:739	 0	738:739	 0	738:739	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	11	67	theme	10Alg	1386:1390	arg1	6729T					1415:1419	=KCTC 52773T=KMM 6729T	1398:1419	=KCTC 52773T=KMM 6729T	1398:1419	The type strain is 10Alg 139T (=KCTC 52773T=KMM 6729T).					
29303693	11	67	theme	10Alg	1386:1390	arg1	strain					1376:1381	The type strain	1367:1381	The type strain	1367:1381	The type strain is 10Alg 139T (=KCTC 52773T=KMM 6729T).					
29303693	11	67	theme	10Alg	1386:1390	arg1	139T					1392:1395	10Alg 139T	1386:1395	10Alg 139T (=KCTC 52773T=KMM 6729T)	1386:1420	The type strain is 10Alg 139T (=KCTC 52773T=KMM 6729T).					
29303693	3	68	theme	16S	339:341	arg1	sequences					353:361	16S rRNA gene sequences	339:361	16S rRNA gene sequences	339:361	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	69	theme	sequence	509:516	arg1	similarity					518:527	highest sequence similarity	501:527	highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species	501:631	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	5	70	theme	lipid	776:780	arg1	profile					782:788	The polar lipid profile	766:788	The polar lipid profile	766:788	The polar lipid profile consisted of the major lipids phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids.					
29303693	2	71	attach	isolated	245:252	arg1	buchiensis					292:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	the Pacific red alga Ahnfeltiato buchiensis	259:301	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	2	71	attach	isolated	245:252	arg2	bacterium					200:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium	126:208	A Gram-stain-negative, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 139T, was isolated from the Pacific red alga Ahnfeltiato buchiensis.					
29303693	3	72	theme	rRNA	343:346	arg1	sequences					353:361	16S rRNA gene sequences	339:361	16S rRNA gene sequences	339:361	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	73	theme	butkevichii	545:555	arg1	3938T					561:565	Polaribacter butkevichii KMM 3938T	532:565	Polaribacter butkevichii KMM 3938T (99.3 %)	532:574	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	3	73	theme	butkevichii	545:555	arg1	%					573:573	99.3 %	568:573	99.3 %	568:573	The phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain belonged to the genus Polaribacter, a member of the family Flavobacteriaceae, the phylum Bacteroidetes, with highest sequence similarity to Polaribacter butkevichii KMM 3938T (99.3 %) and 93.3-98.6 % to other recognized Polaribacter species.					
29303693	1	74	theme	polysaccharide-degrading	33:56	arg1	bacterium					65:73	a polysaccharide-degrading marine bacterium	31:73	a polysaccharide-degrading marine bacterium	31:73	nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis.					
29303693	1	74	theme	polysaccharide-degrading	33:56	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a polysaccharide-degrading marine bacterium isolated from the red alga Ahnfeltia tobuchiensis.					
29303693	4	75	theme	10Alg	670:674	arg1	139T					676:679	strain 10Alg 139T	663:679	strain 10Alg 139T	663:679	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	76	dep	iso-C15 	686:693	arg1	acids					654:658	The prevalent fatty acids	634:658	The prevalent fatty acids of strain 10Alg 139T	634:679	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	76	dep	iso-C15 	686:693	arg1	iso-C15 					686:693	iso-C15 	686:693	iso-C15 	686:693	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
29303693	4	76	dep	iso-C15 	686:693	arg1	3-OH					698:701	 0 3-OH	695:701	 0 3-OH	695:701	The prevalent fatty acids of strain 10Alg 139T were iso-C15 : 0 3-OH, C15 : 0 3-OH, iso-C15:0, iso-C13 : 0, C15 : 0 and C15 : 1ω6c.					
30543278	4	0	theme	cell	932:935	arg1	morphology					937:946	aligned cell morphology	924:946	aligned cell morphology	924:946	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	1	theme	NRCMs	1131:1135	arg1	markers					1060:1066	cardiac and angiogenic specific markers	1028:1066	cardiac and angiogenic specific markers	1028:1066	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	1	theme	NRCMs	1131:1135	arg1	transients					1117:1126	better Ca2+ transients	1105:1126	better Ca2+ transients	1105:1126	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	1	theme	NRCMs	1131:1135	arg1	structure					1090:1098	improved myofilament structure	1069:1098	improved myofilament structure	1069:1098	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	3	2	theme	electrospun	711:721	arg1	nanofibers					723:732	the controllable aligned chitosan electrospun nanofibers	677:732	the controllable aligned chitosan electrospun nanofibers	677:732	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	6	3	theme	composite	1578:1586	arg1	scaffold					1621:1628	a promising scaffold	1609:1628	a promising scaffold for repair of infarcted myocardium	1609:1663	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	6	3	theme	composite	1578:1586	arg1	patch					1596:1600	the CS/chitosan composite cardiac patch	1562:1600	the CS/chitosan composite cardiac patch	1562:1600	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	5	4	theme	CS/chitosan	1288:1298	arg1	patch					1308:1312	the NRCM-seeded aligned CS/chitosan cardiac patch	1264:1312	the NRCM-seeded aligned CS/chitosan cardiac patch	1264:1312	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	3	5	theme	cardiac	762:768	arg1	scaffolds					776:784	the composite cardiac patch scaffolds	748:784	the composite cardiac patch scaffolds	748:784	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	1	6	theme	positive	292:299	arg1	effects					301:307	positive effects	292:307	positive effects	292:307	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	4	7	theme	endothelial	901:911	arg1	cells					913:917	endothelial cells	901:917	endothelial cells	901:917	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	8	theme	disordered	1195:1204	arg1	structures					1206:1215	disordered structures	1195:1215	disordered structures	1195:1215	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	6	9	theme	myocardium	1654:1663	arg1	repair					1634:1639	repair	1634:1639	repair of infarcted myocardium	1634:1663	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	4	10	theme	CS	1174:1175	arg1	component					1177:1185	CS component	1174:1185	CS component	1174:1185	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	0	11	theme	Bioactive	143:151	arg1	Ions					153:156	Bioactive Ions	143:156	Bioactive Ions	143:156	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	0	12	theme	Nanostructure	170:182	arg1	Effect					133:138	Synergistic Effect	121:138	Synergistic Effect of Bioactive Ions and Aligned Nanostructure	121:182	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	3	13	theme	optimized	604:612	arg1	concentrations					614:627	the optimized concentrations	600:627	the optimized concentrations of calcium silicate (CS)	600:652	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	4	14	with	cardiomyocytes	882:895	arg1	morphology					937:946	aligned cell morphology	924:946	aligned cell morphology	924:946	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	1	15	contain	has	288:290	arg2	effects					301:307	positive effects	292:307	positive effects	292:307	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	1	15	contain	has	288:290	arg1	scaffold					274:281	a cell-inductive scaffold	257:281	a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair	257:359	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	4	16	theme	markers	1060:1066	arg1	expressions					1013:1023	upregulated expressions	1001:1023	upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs	1001:1135	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	0	17	theme	Myocardial	78:87	arg1	Performance					89:99	Myocardial Performance	78:99	Myocardial Performance after Infarction	78:116	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	3	18	theme	silicate	640:647	arg1	concentrations					614:627	the optimized concentrations	600:627	the optimized concentrations of calcium silicate (CS)	600:652	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	2	19	theme	neonatal	508:515	arg1	NRCMs					537:541	NRCMs	537:541	NRCMs	537:541	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	2	19	theme	neonatal	508:515	arg1	cardiomyocytes					521:534	neonatal rat cardiomyocytes	508:534	neonatal rat cardiomyocytes (NRCMs)	508:542	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	4	20	with	free	1166:1169	arg1	structures					1206:1215	disordered structures	1195:1215	disordered structures	1195:1215	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	1	21	theme	tissue	193:198	arg1	CTE					213:215	CTE	213:215	CTE	213:215	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	1	21	theme	tissue	193:198	arg1	engineering					200:210	Cardiac tissue engineering	185:210	Cardiac tissue engineering (CTE)	185:216	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	4	22	theme	cardiac	1028:1034	arg1	markers					1060:1066	cardiac and angiogenic specific markers	1028:1066	cardiac and angiogenic specific markers	1028:1066	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	23	theme	synergistic	810:820	arg1	effect					822:827	synergistic effect	810:827	synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology	810:946	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	5	24	theme	scar	1367:1370	arg1	area					1372:1375	the scar area	1363:1375	the scar area	1363:1375	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	4	25	theme	angiogenic	1040:1049	arg1	markers					1060:1066	cardiac and angiogenic specific markers	1028:1066	cardiac and angiogenic specific markers	1028:1066	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	26	theme	Ca2+	1112:1115	arg1	transients					1117:1126	better Ca2+ transients	1105:1126	better Ca2+ transients	1105:1126	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	5	27	theme	NRCM-seeded	1268:1278	arg1	patch					1308:1312	the NRCM-seeded aligned CS/chitosan cardiac patch	1264:1312	the NRCM-seeded aligned CS/chitosan cardiac patch	1264:1312	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	2	28	theme	cardiomyocytes	521:534	arg1	expression					450:459	the expression	446:459	the expression of cardiac-specific genes	446:485	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	2	28	theme	cardiomyocytes	521:534	arg1	proliferation					491:503	proliferation	491:503	proliferation of neonatal rat cardiomyocytes (NRCMs)	491:542	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	2	29	theme	first	388:392	arg1	time					394:397	the first time	384:397	the first time	384:397	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	0	30	theme	Chitosan/Calcium	0:15	arg1	Patch					34:38	Chitosan/Calcium Silicate Cardiac Patch	0:38	Chitosan/Calcium Silicate Cardiac Patch	0:38	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	5	31	theme	postmyocardial	1407:1420	arg1	infarction					1422:1431	postmyocardial infarction	1407:1431	postmyocardial infarction rats	1407:1436	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	0	32	theme	Cardiac	26:32	arg1	Patch					34:38	Chitosan/Calcium Silicate Cardiac Patch	0:38	Chitosan/Calcium Silicate Cardiac Patch	0:38	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	1	33	theme	cardiac	312:318	arg1	behaviors					325:333	cardiac cell behaviors	312:333	cardiac cell behaviors	312:333	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	4	34	theme	structure	1090:1098	arg1	expressions					1013:1023	upregulated expressions	1001:1023	upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs	1001:1135	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	2	35	theme	Si	417:418	arg1	ions					420:423	Si ions	417:423	Si ions	417:423	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	6	36	theme	biomaterials	1537:1548	arg1	application					1488:1498	the potential application	1474:1498	the potential application of bioactive ions and nanostructured biomaterials in CTE	1474:1555	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	5	37	theme	in	1222:1223	arg1	studies					1230:1236	The in vivo studies	1218:1236	The in vivo studies	1218:1236	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	2	38	theme	genes	481:485	arg1	expression					450:459	the expression	446:459	the expression of cardiac-specific genes	446:485	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	2	38	theme	genes	481:485	arg1	proliferation					491:503	proliferation	491:503	proliferation of neonatal rat cardiomyocytes (NRCMs)	491:542	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	2	39	theme	rat	517:519	arg1	NRCMs					537:541	NRCMs	537:541	NRCMs	537:541	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	2	39	theme	rat	517:519	arg1	cardiomyocytes					521:534	neonatal rat cardiomyocytes	508:534	neonatal rat cardiomyocytes (NRCMs)	508:542	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	1	40	theme	cardiac	339:345	arg1	repair					354:359	cardiac tissue repair	339:359	cardiac tissue repair	339:359	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	3	41	theme	chitosan	702:709	arg1	nanofibers					723:732	the controllable aligned chitosan electrospun nanofibers	677:732	the controllable aligned chitosan electrospun nanofibers	677:732	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	4	42	theme	chemical	842:849	arg1	signals					866:872	bioactive chemical and structural signals	832:872	bioactive chemical and structural signals	832:872	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	3	43	theme	controllable	681:692	arg1	nanofibers					723:732	the controllable aligned chitosan electrospun nanofibers	677:732	the controllable aligned chitosan electrospun nanofibers	677:732	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	4	44	theme	structural	855:864	arg1	signals					866:872	bioactive chemical and structural signals	832:872	bioactive chemical and structural signals	832:872	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	5	45	theme	cardiac	1333:1339	arg1	function					1341:1348	cardiac function	1333:1348	cardiac function	1333:1348	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	4	46	theme	improved	1069:1076	arg1	structure					1090:1098	improved myofilament structure	1069:1098	improved myofilament structure	1069:1098	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	6	47	theme	cardiac	1588:1594	arg1	scaffold					1621:1628	a promising scaffold	1609:1628	a promising scaffold for repair of infarcted myocardium	1609:1663	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	6	47	theme	cardiac	1588:1594	arg1	patch					1596:1600	the CS/chitosan composite cardiac patch	1562:1600	the CS/chitosan composite cardiac patch	1562:1600	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	5	48	theme	aligned	1280:1286	arg1	patch					1308:1312	the NRCM-seeded aligned CS/chitosan cardiac patch	1264:1312	the NRCM-seeded aligned CS/chitosan cardiac patch	1264:1312	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	4	49	theme	aligned	924:930	arg1	morphology					937:946	aligned cell morphology	924:946	aligned cell morphology	924:946	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	6	50	theme	CS/chitosan	1566:1576	arg1	scaffold					1621:1628	a promising scaffold	1609:1628	a promising scaffold for repair of infarcted myocardium	1609:1663	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	6	50	theme	CS/chitosan	1566:1576	arg1	patch					1596:1600	the CS/chitosan composite cardiac patch	1562:1600	the CS/chitosan composite cardiac patch	1562:1600	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	5	51	theme	cardiac	1300:1306	arg1	patch					1308:1312	the NRCM-seeded aligned CS/chitosan cardiac patch	1264:1312	the NRCM-seeded aligned CS/chitosan cardiac patch	1264:1312	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	3	52	theme	patch	770:774	arg1	scaffolds					776:784	the composite cardiac patch scaffolds	748:784	the composite cardiac patch scaffolds	748:784	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	2	53	theme	cardiac-specific	464:479	arg1	genes					481:485	cardiac-specific genes	464:485	cardiac-specific genes	464:485	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	3	54	theme	composite	752:760	arg1	scaffolds					776:784	the composite cardiac patch scaffolds	748:784	the composite cardiac patch scaffolds	748:784	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	6	55	theme	infarcted	1644:1652	arg1	myocardium					1654:1663	infarcted myocardium	1644:1663	infarcted myocardium	1644:1663	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	5	56	from	angiogenesis	1391:1402	arg1	rats					1433:1436	postmyocardial infarction rats	1407:1436	postmyocardial infarction rats	1407:1436	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	4	57	theme	component	1177:1185	arg1	free					1166:1169	free	1166:1169	free	1166:1169	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	0	58	theme	Ions	153:156	arg1	Effect					133:138	Synergistic Effect	121:138	Synergistic Effect of Bioactive Ions and Aligned Nanostructure	121:182	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	0	59	theme	Aligned	162:168	arg1	Nanostructure					170:182	Aligned Nanostructure	162:182	Aligned Nanostructure	162:182	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	1	60	theme	great	228:232	arg1	challenge					234:242	a great challenge	226:242	a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair	226:359	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	4	61	theme	upregulated	1001:1011	arg1	expressions					1013:1023	upregulated expressions	1001:1023	upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs	1001:1135	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	6	62	theme	potential	1478:1486	arg1	application					1488:1498	the potential application	1474:1498	the potential application of bioactive ions and nanostructured biomaterials in CTE	1474:1555	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	2	63	theme	ppm	582:584	arg1	ranges					561:566	concentration ranges	547:566	concentration ranges of 0.13-10.78 ppm	547:584	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	6	64	theme	ions	1513:1516	arg1	application					1488:1498	the potential application	1474:1498	the potential application of bioactive ions and nanostructured biomaterials in CTE	1474:1555	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	3	65	theme	calcium	632:638	arg1	silicate					640:647	calcium silicate	632:647	calcium silicate (CS)	632:652	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	3	65	theme	calcium	632:638	arg1	CS					650:651	CS	650:651	CS	650:651	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	4	66	theme	specific	1051:1058	arg1	markers					1060:1066	cardiac and angiogenic specific markers	1028:1066	cardiac and angiogenic specific markers	1028:1066	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	6	67	from	application	1488:1498	arg1	CTE					1553:1555	CTE	1553:1555	CTE	1553:1555	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	1	68	theme	Cardiac	185:191	arg1	CTE					213:215	CTE	213:215	CTE	213:215	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	1	68	theme	Cardiac	185:191	arg1	engineering					200:210	Cardiac tissue engineering	185:210	Cardiac tissue engineering (CTE)	185:216	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	4	69	with	cells	913:917	arg1	morphology					937:946	aligned cell morphology	924:946	aligned cell morphology	924:946	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	6	70	theme	bioactive	1503:1511	arg1	ions					1513:1516	bioactive ions	1503:1516	bioactive ions	1503:1516	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	0	71	theme	Synergistic	121:131	arg1	Effect					133:138	Synergistic Effect	121:138	Synergistic Effect of Bioactive Ions and Aligned Nanostructure	121:182	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	4	72	from	effect	822:827	arg1	cells					913:917	endothelial cells	901:917	endothelial cells	901:917	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	72	from	effect	822:827	arg1	cardiomyocytes					882:895	cardiomyocytes	882:895	cardiomyocytes	882:895	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	5	73	dep	in	1222:1223	arg1	vivo					1225:1228	vivo	1225:1228	vivo	1225:1228	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	0	74	theme	Silicate	17:24	arg1	Patch					34:38	Chitosan/Calcium Silicate Cardiac Patch	0:38	Chitosan/Calcium Silicate Cardiac Patch	0:38	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	4	75	theme	better	1105:1110	arg1	transients					1117:1126	better Ca2+ transients	1105:1126	better Ca2+ transients	1105:1126	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	76	theme	transients	1117:1126	arg1	expressions					1013:1023	upregulated expressions	1001:1023	upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs	1001:1135	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	5	77	theme	infarction	1422:1431	arg1	rats					1433:1436	postmyocardial infarction rats	1407:1436	postmyocardial infarction rats	1407:1436	The in vivo studies further demonstrated that the NRCM-seeded aligned CS/chitosan cardiac patch evidently improved cardiac function via limiting the scar area and promoting angiogenesis in postmyocardial infarction rats.					
30543278	0	78	theme	Cardiomyocyte	51:63	arg1	Activity					65:72	Cardiomyocyte Activity	51:72	Cardiomyocyte Activity	51:72	Chitosan/Calcium Silicate Cardiac Patch Stimulates Cardiomyocyte Activity and Myocardial Performance after Infarction by Synergistic Effect of Bioactive Ions and Aligned Nanostructure.					
30543278	1	79	theme	cell	320:323	arg1	behaviors					325:333	cardiac cell behaviors	312:333	cardiac cell behaviors	312:333	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	4	80	theme	myofilament	1078:1088	arg1	structure					1090:1098	improved myofilament structure	1069:1098	improved myofilament structure	1069:1098	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	81	theme	signals	866:872	arg1	effect					822:827	synergistic effect	810:827	synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology	810:946	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	6	82	theme	nanostructured	1522:1535	arg1	biomaterials					1537:1548	nanostructured biomaterials	1522:1548	nanostructured biomaterials	1522:1548	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	2	83	theme	concentration	547:559	arg1	ranges					561:566	concentration ranges	547:566	concentration ranges of 0.13-10.78 ppm	547:584	In this study, we for the first time demonstrated that Si ions evidently stimulated the expression of cardiac-specific genes and proliferation of neonatal rat cardiomyocytes (NRCMs) at concentration ranges of 0.13-10.78 ppm.					
30543278	3	84	theme	aligned	694:700	arg1	nanofibers					723:732	the controllable aligned chitosan electrospun nanofibers	677:732	the controllable aligned chitosan electrospun nanofibers	677:732	Accordingly, the optimized concentrations of calcium silicate (CS) were incorporated into the controllable aligned chitosan electrospun nanofibers, constructing the composite cardiac patch scaffolds.					
30543278	6	85	theme	promising	1611:1619	arg1	scaffold					1621:1628	a promising scaffold	1609:1628	a promising scaffold for repair of infarcted myocardium	1609:1663	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	6	85	theme	promising	1611:1619	arg1	patch					1596:1600	the CS/chitosan composite cardiac patch	1562:1600	the CS/chitosan composite cardiac patch	1562:1600	Conclusively, our study highlights the potential application of bioactive ions and nanostructured biomaterials in CTE, and the CS/chitosan composite cardiac patch may be a promising scaffold for repair of infarcted myocardium.					
30543278	1	86	theme	tissue	347:352	arg1	repair					354:359	cardiac tissue repair	339:359	cardiac tissue repair	339:359	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
30543278	4	87	theme	bioactive	832:840	arg1	signals					866:872	bioactive chemical and structural signals	832:872	bioactive chemical and structural signals	832:872	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	4	88	theme	free	1166:1169	arg1	scaffolds					1156:1164	the scaffolds	1152:1164	the scaffolds free of CS component or with disordered structures	1152:1215	These scaffolds showed synergistic effect of bioactive chemical and structural signals on both cardiomyocytes and endothelial cells with aligned cell morphology and enhanced viability and function characterized by upregulated expressions of cardiac and angiogenic specific markers, improved myofilament structure, and better Ca2+ transients of NRCMs as compared to the scaffolds free of CS component or with disordered structures.					
30543278	1	89	theme	cell-inductive	259:272	arg1	scaffold					274:281	a cell-inductive scaffold	257:281	a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair	257:359	Cardiac tissue engineering (CTE) remains a great challenge to construct a cell-inductive scaffold that has positive effects on cardiac cell behaviors and cardiac tissue repair.					
31690674	6	0	theme	locus	1159:1163	arg1	PUL					1166:1168	PUL	1166:1168	PUL	1166:1168	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	6	0	theme	locus	1159:1163	arg1	proteins					1116:1123	proteins	1116:1123	proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide	1116:1261	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	4	1	theme	ex	590:591	arg1	assay					606:610	a small intestinal ex vivo culture assay	571:610	a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice	571:662	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	3	2	theme	microbiota	517:526	arg1	antigens					528:535	gut microbiota antigens	513:535	gut microbiota antigens targeted by gut IgA	513:555	This study provides a framework for identifying and characterizing gut microbiota antigens targeted by gut IgA.					
31690674	4	3	theme	culture	598:604	arg1	assay					606:610	a small intestinal ex vivo culture assay	571:610	a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice	571:662	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	9	4	theme	protein	2152:2158	arg1	complex					2160:2166	a protein complex	2150:2166	a protein complex involved in the utilization of an important dietary polysaccharide: fructan	2150:2242	In our present study, we discovered that gut IgA targeted a protein complex involved in the utilization of an important dietary polysaccharide: fructan.					
31690674	6	5	with	membrane	1021:1028	arg1	functionalities					1065:1079	diverse functionalities	1057:1079	diverse functionalities	1057:1079	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	10	6	theme	microbial	2506:2514	arg1	utilization					2516:2526	the microbial utilization	2502:2526	the microbial utilization of such polysaccharides	2502:2550	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	3	7	theme	gut	549:551	arg1	IgA					553:555	gut IgA	549:555	gut IgA	549:555	This study provides a framework for identifying and characterizing gut microbiota antigens targeted by gut IgA.					
31690674	5	8	theme	diverse	884:890	arg1	polysaccharides					921:935	capsular polysaccharides	912:935	capsular polysaccharides	912:935	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	5	8	theme	diverse	884:890	arg1	antigens					892:899	diverse antigens	884:899	diverse antigens	884:899	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	5	8	theme	diverse	884:890	arg1	lipopolysaccharides					938:956	lipopolysaccharides	938:956	lipopolysaccharides	938:956	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	5	8	theme	diverse	884:890	arg1	proteins					963:970	proteins	963:970	proteins	963:970	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	10	9	theme	unrestricted	2316:2327	arg1	growth					2329:2334	unrestricted growth	2316:2334	unrestricted growth of fructan-utilizing bacteria	2316:2364	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	6	10	theme	fructan	1209:1215	arg1	polysaccharide					1248:1261	an important dietary polysaccharide	1227:1261	an important dietary polysaccharide	1227:1261	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	6	10	theme	fructan	1209:1215	arg1	utilization					1194:1204	utilization	1194:1204	utilization	1194:1204	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	10	11	theme	polysaccharides	2536:2550	arg1	utilization					2516:2526	the microbial utilization	2502:2526	the microbial utilization of such polysaccharides	2502:2550	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	7	12	theme	novel	1663:1667	arg1	role					1669:1672	a novel role	1661:1672	a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health	1661:1829	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	10	13	theme	bacteria	2357:2364	arg1	growth					2329:2334	unrestricted growth	2316:2334	unrestricted growth of fructan-utilizing bacteria	2316:2364	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	7	14	theme	B.	1443:1444	arg1	thetaiotaomicron					1446:1461	B. thetaiotaomicron	1443:1461	B. thetaiotaomicron	1443:1461	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	4	15	theme	gnotobiotic	647:657	arg1	mice					659:662	gnotobiotic mice	647:662	gnotobiotic mice	647:662	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	9	16	theme	important	2202:2210	arg1	polysaccharide					2220:2233	an important dietary polysaccharide	2199:2233	an important dietary polysaccharide	2199:2233	In our present study, we discovered that gut IgA targeted a protein complex involved in the utilization of an important dietary polysaccharide: fructan.					
31690674	4	17	dep	ex	590:591	arg1	vivo					593:596	vivo	593:596	vivo	593:596	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	10	18	theme	gut	2387:2389	arg1	IgA					2391:2393	gut IgA	2387:2393	gut IgA	2387:2393	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	7	19	theme	PUL	1436:1438	arg1	expression					1414:1423	the expression	1410:1423	the expression of fructan PUL in B. thetaiotaomicron	1410:1461	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	9	20	theme	polysaccharide	2220:2233	arg1	utilization					2184:2194	the utilization	2180:2194	the utilization of an important dietary polysaccharide	2180:2233	In our present study, we discovered that gut IgA targeted a protein complex involved in the utilization of an important dietary polysaccharide: fructan.					
31690674	1	21	theme	Gut-derived	140:150	arg1	IgA					170:172	IgA	170:172	IgA	170:172	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	1	21	theme	Gut-derived	140:150	arg1	A					167:167	Gut-derived immunoglobulin A	140:167	Gut-derived immunoglobulin A (IgA)	140:173	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	1	21	theme	Gut-derived	140:150	arg1	antibody					196:203	the most abundant antibody	178:203	the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality	178:280	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	9	22	dep	complex	2160:2166	arg1	fructan					2236:2242	fructan	2236:2242	a protein complex involved in the utilization of an important dietary polysaccharide: fructan	2150:2242	In our present study, we discovered that gut IgA targeted a protein complex involved in the utilization of an important dietary polysaccharide: fructan.					
31690674	4	23	theme	model	716:720	arg1	commensal					732:740	a model human gut commensal	714:740	a model human gut commensal	714:740	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	4	23	theme	model	716:720	arg1	thetaiotaomicron					755:770	Bacteroides thetaiotaomicron VPI-5482	743:779	Bacteroides thetaiotaomicron VPI-5482	743:779	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	8	24	theme	diet	1980:1983	arg1	impact					1970:1975	the impact	1966:1975	the impact of diet on gut microbiota	1966:2001	While many studies have highlighted the impact of diet on gut microbiota, little is known about how the host regulates this critical diet-microbiota interaction.					
31690674	7	25	theme	significant	1775:1785	arg1	impact					1787:1792	the significant impact	1771:1792	the significant impact that gut microbes have on our health	1771:1829	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	7	26	theme	fructans	1633:1640	arg1	presence					1613:1620	the presence	1609:1620	the presence of dietary fructans	1609:1640	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	6	27	theme	dietary	1240:1246	arg1	polysaccharide					1248:1261	an important dietary polysaccharide	1227:1261	an important dietary polysaccharide	1227:1261	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	6	27	theme	dietary	1240:1246	arg1	utilization					1194:1204	utilization	1194:1204	utilization	1194:1204	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	8	28	theme	gut	1988:1990	arg1	microbiota					1992:2001	gut microbiota	1988:2001	gut microbiota	1988:2001	While many studies have highlighted the impact of diet on gut microbiota, little is known about how the host regulates this critical diet-microbiota interaction.					
31690674	5	29	theme	capsular	912:919	arg1	polysaccharides					921:935	capsular polysaccharides	912:935	capsular polysaccharides	912:935	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	6	30	theme	diverse	1057:1063	arg1	functionalities					1065:1079	diverse functionalities	1057:1079	diverse functionalities	1057:1079	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	4	31	theme	antigenic	693:701	arg1	targets					703:709	antigenic targets	693:709	antigenic targets	693:709	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	10	32	theme	fructan	2434:2440	arg1	utilization					2442:2452	fructan utilization	2434:2452	fructan utilization	2434:2452	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	7	33	dep	impact	1787:1792	arg1	have					1812:1815	have	1812:1815	have on our health	1812:1829	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	0	34	theme	Gut	96:98	arg1	thetaiotaomicron					122:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	10	35	theme	responsible	2418:2428	arg1	proteins					2409:2416	proteins	2409:2416	proteins responsible for fructan utilization	2409:2452	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	7	36	theme	IgA	1380:1382	arg1	production					1345:1354	the production	1341:1354	the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron,	1341:1462	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	2	37	theme	IgA	389:391	arg1	effects					378:384	the functional effects	363:384	the functional effects of IgA targeting these antigens	363:416	However, most of the microbial antigens targeted by gut IgA remain unknown, and the functional effects of IgA targeting these antigens are currently understudied.					
31690674	0	38	theme	Bacteroides	110:120	arg1	thetaiotaomicron					122:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	7	39	theme	microbial	1700:1708	arg1	colonization					1710:1721	microbial colonization	1700:1721	microbial colonization	1700:1721	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	10	40	theme	dietary	2267:2273	arg1	fructans					2275:2282	dietary fructans	2267:2282	dietary fructans	2267:2282	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	4	41	theme	gut	728:730	arg1	commensal					732:740	a model human gut commensal	714:740	a model human gut commensal	714:740	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	4	41	theme	gut	728:730	arg1	thetaiotaomicron					755:770	Bacteroides thetaiotaomicron VPI-5482	743:779	Bacteroides thetaiotaomicron VPI-5482	743:779	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	0	42	theme	Intestinal	0:9	arg1	IgA					11:13	Intestinal IgA	0:13	Intestinal IgA	0:13	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	5	43	theme	IgA	845:847	arg1	response					849:856	a microbe-specific IgA response	826:856	a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins	826:970	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	5	43	theme	IgA	845:847	arg1	reactive					867:874	reactive	867:874	reactive	867:874	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	7	44	from	gut	1602:1604	arg1	presence					1613:1620	the presence	1609:1620	the presence of dietary fructans	1609:1640	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	7	45	theme	gut	1678:1680	arg1	IgA					1682:1684	gut IgA	1678:1684	gut IgA	1678:1684	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	6	46	theme	specific	1099:1106	arg1	IgA					1095:1097	an IgA	1092:1097	an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide	1092:1261	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	8	47	theme	critical	2054:2061	arg1	interaction					2079:2089	this critical diet-microbiota interaction	2049:2089	this critical diet-microbiota interaction	2049:2089	While many studies have highlighted the impact of diet on gut microbiota, little is known about how the host regulates this critical diet-microbiota interaction.					
31690674	7	48	theme	B.	1566:1567	arg1	thetaiotaomicron					1569:1584	B. thetaiotaomicron	1566:1584	B. thetaiotaomicron	1566:1584	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	7	49	theme	environmental	1873:1885	arg1	factors					1887:1893	key host and environmental factors	1860:1893	factors	1887:1893	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	7	50	theme	fructan	1359:1365	arg1	IgA					1380:1382	fructan PUL-specific IgA	1359:1382	fructan PUL-specific IgA	1359:1382	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	0	51	theme	Utilization	64:74	arg1	Locus					76:80	a Fructan Polysaccharide Utilization Locus	39:80	a Fructan Polysaccharide Utilization Locus	39:80	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	0	52	theme	Fructan	41:47	arg1	Locus					76:80	a Fructan Polysaccharide Utilization Locus	39:80	a Fructan Polysaccharide Utilization Locus	39:80	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	7	53	theme	diverse	1911:1917	arg1	community					1919:1927	this diverse community	1906:1927	this diverse community	1906:1927	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	6	54	theme	important	1230:1238	arg1	polysaccharide					1248:1261	an important dietary polysaccharide	1227:1261	an important dietary polysaccharide	1227:1261	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	6	54	theme	important	1230:1238	arg1	utilization					1194:1204	utilization	1194:1204	utilization	1194:1204	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	2	55	theme	gut	335:337	arg1	IgA					339:341	gut IgA	335:341	gut IgA	335:341	However, most of the microbial antigens targeted by gut IgA remain unknown, and the functional effects of IgA targeting these antigens are currently understudied.					
31690674	1	56	theme	gut	237:239	arg1	composition					252:262	gut microbiota composition	237:262	gut microbiota composition	237:262	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	0	57	theme	Polysaccharide	49:62	arg1	Locus					76:80	a Fructan Polysaccharide Utilization Locus	39:80	a Fructan Polysaccharide Utilization Locus	39:80	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	7	58	theme	dietary	1315:1321	arg1	fructan					1323:1329	dietary fructan	1315:1329	dietary fructan	1315:1329	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	4	59	theme	harvest	615:621	arg1	propria					630:636	harvest lamina propria	615:636	harvest lamina propria IgA from gnotobiotic mice	615:662	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	7	60	theme	Further	1264:1270	arg1	analyses					1272:1279	Further analyses	1264:1279	Further analyses	1264:1279	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	3	61	theme	gut	513:515	arg1	antigens					528:535	gut microbiota antigens	513:535	gut microbiota antigens targeted by gut IgA	513:555	This study provides a framework for identifying and characterizing gut microbiota antigens targeted by gut IgA.					
31690674	7	62	theme	PUL	1522:1524	arg1	expression					1500:1509	the expression	1496:1509	the expression of fructan PUL	1496:1524	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	6	63	theme	utilization	1147:1157	arg1	locus					1159:1163	the polysaccharide utilization locus	1128:1163	the polysaccharide utilization locus	1128:1163	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	4	64	theme	intestinal	579:588	arg1	assay					606:610	a small intestinal ex vivo culture assay	571:610	a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice	571:662	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	8	65	from	impact	1970:1975	arg1	microbiota					1992:2001	gut microbiota	1988:2001	gut microbiota	1988:2001	While many studies have highlighted the impact of diet on gut microbiota, little is known about how the host regulates this critical diet-microbiota interaction.					
31690674	8	66	theme	many	1936:1939	arg1	studies					1941:1947	many studies	1936:1947	many studies	1936:1947	While many studies have highlighted the impact of diet on gut microbiota, little is known about how the host regulates this critical diet-microbiota interaction.					
31690674	4	67	from	mice	659:662	arg1	IgA					638:640	harvest lamina propria IgA	615:640	harvest lamina propria IgA from gnotobiotic mice	615:662	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	1	68	theme	abundant	187:194	arg1	antibody					196:203	the most abundant antibody	178:203	the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality	178:280	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	1	68	theme	abundant	187:194	arg1	A					167:167	Gut-derived immunoglobulin A	140:167	Gut-derived immunoglobulin A (IgA)	140:173	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	2	69	theme	microbial	304:312	arg1	antigens					314:321	the microbial antigens	300:321	the microbial antigens targeted by gut IgA	300:341	However, most of the microbial antigens targeted by gut IgA remain unknown, and the functional effects of IgA targeting these antigens are currently understudied.					
31690674	4	70	theme	propria	630:636	arg1	IgA					638:640	harvest lamina propria IgA	615:640	harvest lamina propria IgA from gnotobiotic mice	615:662	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	6	71	theme	microbial	985:993	arg1	antigens					1003:1010	microbial protein antigens	985:1010	microbial protein antigens	985:1010	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	10	72	theme	fructan-utilizing	2339:2355	arg1	bacteria					2357:2364	fructan-utilizing bacteria	2339:2364	fructan-utilizing bacteria	2339:2364	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	9	73	theme	dietary	2212:2218	arg1	polysaccharide					2220:2233	an important dietary polysaccharide	2199:2233	an important dietary polysaccharide	2199:2233	In our present study, we discovered that gut IgA targeted a protein complex involved in the utilization of an important dietary polysaccharide: fructan.					
31690674	1	74	theme	immunoglobulin	152:165	arg1	IgA					170:172	IgA	170:172	IgA	170:172	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	1	74	theme	immunoglobulin	152:165	arg1	A					167:167	Gut-derived immunoglobulin A	140:167	Gut-derived immunoglobulin A (IgA)	140:173	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	1	74	theme	immunoglobulin	152:165	arg1	antibody					196:203	the most abundant antibody	178:203	the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality	178:280	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	7	75	theme	gut	1799:1801	arg1	microbes					1803:1810	gut microbes	1799:1810	gut microbes	1799:1810	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	7	76	theme	dietary	1625:1631	arg1	fructans					1633:1640	dietary fructans	1625:1640	dietary fructans	1625:1640	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	0	77	theme	Locus	76:80	arg1	Expression					25:34	Expression	25:34	Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron	25:137	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	10	78	with	host	2468:2471	arg1	tools					2478:2482	tools	2478:2482	tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth	2478:2584	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	0	79	from	Expression	25:34	arg1	thetaiotaomicron					122:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	6	80	with	proteins	1043:1050	arg1	functionalities					1065:1079	diverse functionalities	1057:1079	diverse functionalities	1057:1079	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	0	81	theme	Colonizing	85:94	arg1	thetaiotaomicron					122:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	0	82	theme	Commensal	100:108	arg1	thetaiotaomicron					122:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Colonizing Gut Commensal Bacteroides thetaiotaomicron	85:137	Intestinal IgA Regulates Expression of a Fructan Polysaccharide Utilization Locus in Colonizing Gut Commensal Bacteroides thetaiotaomicron.					
31690674	6	83	theme	secreted	1034:1041	arg1	proteins					1043:1050	secreted proteins	1034:1050	secreted proteins	1034:1050	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	6	83	theme	secreted	1034:1041	arg1	IgA					1095:1097	an IgA	1092:1097	an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide	1092:1261	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	4	84	theme	Bacteroides	743:753	arg1	commensal					732:740	a model human gut commensal	714:740	a model human gut commensal	714:740	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	4	84	theme	Bacteroides	743:753	arg1	thetaiotaomicron					755:770	Bacteroides thetaiotaomicron VPI-5482	743:779	Bacteroides thetaiotaomicron VPI-5482	743:779	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	10	85	theme	such	2531:2534	arg1	polysaccharides					2536:2550	such polysaccharides	2531:2550	such polysaccharides	2531:2550	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	7	86	from	expression	1414:1423	arg1	thetaiotaomicron					1446:1461	B. thetaiotaomicron	1443:1461	B. thetaiotaomicron	1443:1461	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	6	87	theme	polysaccharide	1132:1145	arg1	locus					1159:1163	the polysaccharide utilization locus	1128:1163	the polysaccharide utilization locus	1128:1163	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	10	88	theme	fructans	2275:2282	arg1	presence					2255:2262	the presence	2251:2262	the presence of dietary fructans	2251:2282	While the presence of dietary fructans was previously thought to allow unrestricted growth of fructan-utilizing bacteria, our work shows that gut IgA, by targeting proteins responsible for fructan utilization, provides the host with tools that can restrict the microbial utilization of such polysaccharides, thereby controlling their growth.					
31690674	6	89	theme	protein	995:1001	arg1	antigens					1003:1010	microbial protein antigens	985:1010	microbial protein antigens	985:1010	IgA against microbial protein antigens targeted membrane and secreted proteins with diverse functionalities, including an IgA specific against proteins of the polysaccharide utilization locus (PUL) that are necessary for utilization of fructan, which is an important dietary polysaccharide.					
31690674	4	90	theme	human	722:726	arg1	commensal					732:740	a model human gut commensal	714:740	a model human gut commensal	714:740	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	4	90	theme	human	722:726	arg1	thetaiotaomicron					755:770	Bacteroides thetaiotaomicron VPI-5482	743:779	Bacteroides thetaiotaomicron VPI-5482	743:779	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	2	91	theme	functional	367:376	arg1	effects					378:384	the functional effects	363:384	the functional effects of IgA targeting these antigens	363:416	However, most of the microbial antigens targeted by gut IgA remain unknown, and the functional effects of IgA targeting these antigens are currently understudied.					
31690674	5	92	theme	microbe-specific	828:843	arg1	response					849:856	a microbe-specific IgA response	826:856	a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins	826:970	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	5	92	theme	microbe-specific	828:843	arg1	reactive					867:874	reactive	867:874	reactive	867:874	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	7	93	theme	PUL-specific	1367:1378	arg1	IgA					1380:1382	fructan PUL-specific IgA	1359:1382	fructan PUL-specific IgA	1359:1382	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	4	94	theme	small	573:577	arg1	assay					606:610	a small intestinal ex vivo culture assay	571:610	a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice	571:662	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	8	95	theme	diet-microbiota	2063:2077	arg1	interaction					2079:2089	this critical diet-microbiota interaction	2049:2089	this critical diet-microbiota interaction	2049:2089	While many studies have highlighted the impact of diet on gut microbiota, little is known about how the host regulates this critical diet-microbiota interaction.					
31690674	9	96	theme	present	2099:2105	arg1	study					2107:2111	our present study	2095:2111	our present study	2095:2111	In our present study, we discovered that gut IgA targeted a protein complex involved in the utilization of an important dietary polysaccharide: fructan.					
31690674	5	97	theme	B.	798:799	arg1	thetaiotaomicron					801:816	B. thetaiotaomicron	798:816	B. thetaiotaomicron	798:816	Colonization by B. thetaiotaomicron induced a microbe-specific IgA response that was reactive against diverse antigens, including capsular polysaccharides, lipopolysaccharides, and proteins.					
31690674	7	98	theme	thetaiotaomicron	1569:1584	arg1	ability					1555:1561	the ability	1551:1561	the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans	1551:1640	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	7	99	theme	fructan	1323:1329	arg1	presence					1303:1310	the presence	1299:1310	the presence of dietary fructan	1299:1329	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	1	100	link	Gut-derived	140:150	arg1	IgA					170:172	IgA	170:172	IgA	170:172	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	1	100	link	Gut-derived	140:150	arg1	A					167:167	Gut-derived immunoglobulin A	140:167	Gut-derived immunoglobulin A (IgA)	140:173	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	1	100	link	Gut-derived	140:150	arg1	antibody					196:203	the most abundant antibody	178:203	the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality	178:280	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31690674	7	101	theme	key	1860:1862	arg1	host					1864:1867	key host and environmental factors	1860:1893	host	1864:1867	Further analyses demonstrated that the presence of dietary fructan increased the production of fructan PUL-specific IgA, which then downregulated the expression of fructan PUL in B. thetaiotaomicron, both in vivo and in vitro Since the expression of fructan PUL has been associated with the ability of B. thetaiotaomicron to colonize the gut in the presence of dietary fructans, our work suggests a novel role for gut IgA in regulating microbial colonization by modulating their metabolism.IMPORTANCE Given the significant impact that gut microbes have on our health, it is essential to identify key host and environmental factors that shape this diverse community.					
31690674	4	102	theme	lamina	623:628	arg1	propria					630:636	harvest lamina propria	615:636	harvest lamina propria IgA from gnotobiotic mice	615:662	We developed a small intestinal ex vivo culture assay to harvest lamina propria IgA from gnotobiotic mice, with the aim of identifying antigenic targets in a model human gut commensal, Bacteroides thetaiotaomicron VPI-5482.					
31690674	9	103	theme	gut	2133:2135	arg1	IgA					2137:2139	gut IgA	2133:2139	gut IgA	2133:2139	In our present study, we discovered that gut IgA targeted a protein complex involved in the utilization of an important dietary polysaccharide: fructan.					
31690674	1	104	theme	microbiota	241:250	arg1	composition					252:262	gut microbiota composition	237:262	gut microbiota composition	237:262	Gut-derived immunoglobulin A (IgA) is the most abundant antibody secreted in the gut that shapes gut microbiota composition and functionality.					
31490664	8	0	theme	other	1340:1344	arg1	hand					1346:1349	the other hand	1336:1349	the other hand	1336:1349	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	5	1	theme	novel	913:917	arg1	composites					919:928	the novel composites	909:928	the novel composites	909:928	Confirming the cytocompatibility of the novel composites, the viability of encapsulated L929 fibroblasts is >90% in all hydrogels.					
31490664	1	2	theme	biochemical	285:295	arg1	cues					297:300	mechanical and biochemical cues	270:300	mechanical and biochemical cues	270:300	Naturally, cells reside in three-dimensional (3D) microenvironments composed of biopolymers that guide cellular behavior via topographical features as well as through mechanical and biochemical cues.					
31490664	7	3	theme	F-actin/nuclei	1220:1233	arg1	staining					1235:1242	F-actin/nuclei staining	1220:1242	F-actin/nuclei staining	1220:1242	After 4 d of culture, Live/Dead staining and F-actin/nuclei staining show that the encapsulated fibroblasts develop an elongated morphology in the hydrogels.					
31490664	8	4	theme	progenitor	1378:1387	arg1	cells					1395:1399	encapsulated chondrogenic progenitor ATDC5 cells	1352:1399	encapsulated chondrogenic progenitor ATDC5 cells	1352:1399	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	5	5	theme	composites	919:928	arg1	cytocompatibility					888:904	the cytocompatibility	884:904	the cytocompatibility of the novel composites	884:928	Confirming the cytocompatibility of the novel composites, the viability of encapsulated L929 fibroblasts is >90% in all hydrogels.					
31490664	7	6	theme	Live/Dead	1197:1205	arg1	staining					1207:1214	Live/Dead staining	1197:1214	Live/Dead staining	1197:1214	After 4 d of culture, Live/Dead staining and F-actin/nuclei staining show that the encapsulated fibroblasts develop an elongated morphology in the hydrogels.					
31490664	8	7	theme	encapsulated	1352:1363	arg1	cells					1395:1399	encapsulated chondrogenic progenitor ATDC5 cells	1352:1399	encapsulated chondrogenic progenitor ATDC5 cells	1352:1399	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	5	8	from	%	984:984	arg1	hydrogels					993:1001	all hydrogels	989:1001	all hydrogels	989:1001	Confirming the cytocompatibility of the novel composites, the viability of encapsulated L929 fibroblasts is >90% in all hydrogels.					
31490664	4	9	theme	modulus	753:759	arg1	increase					729:736	an increase	726:736	an increase of compressive modulus	726:759	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %) results in an increase of compressive modulus when compared to hydrogels without SNCs, without affecting the swelling ratio, thus providing a tunable system.					
31490664	9	10	theme	powerful	1682:1689	arg1	cues					1640:1643	topographical and mechanical cues	1611:1643	topographical and mechanical cues within 3D microenvironments	1611:1671	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	9	10	theme	powerful	1682:1689	arg1	tool					1691:1694	a powerful tool to instruct cells' behavior	1680:1722	a powerful tool to instruct cells' behavior	1680:1722	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	7	11	theme	culture	1188:1194	arg1	d					1183:1183	4 d	1181:1183	4 d of culture	1181:1194	After 4 d of culture, Live/Dead staining and F-actin/nuclei staining show that the encapsulated fibroblasts develop an elongated morphology in the hydrogels.					
31490664	7	12	theme	elongated	1294:1302	arg1	morphology					1304:1313	an elongated morphology	1291:1313	an elongated morphology	1291:1313	After 4 d of culture, Live/Dead staining and F-actin/nuclei staining show that the encapsulated fibroblasts develop an elongated morphology in the hydrogels.					
31490664	2	13	theme	most	312:315	arg1	studies					317:323	most studies	312:323	most studies describing the influence of topography on cells' behavior	312:381	However, most studies describing the influence of topography on cells' behavior are performed on rigid and synthetic two-dimensional substrates.					
31490664	6	14	theme	cellular	1008:1015	arg1	similar					1056:1062	similar	1056:1062	similar	1056:1062	The cellular metabolic activity and DNA content are similar for all formulations and increase over time, indicating that the fibroblasts proliferate within the hydrogels.					
31490664	6	14	theme	cellular	1008:1015	arg1	activity					1027:1034	The cellular metabolic activity	1004:1034	The cellular metabolic activity	1004:1034	The cellular metabolic activity and DNA content are similar for all formulations and increase over time, indicating that the fibroblasts proliferate within the hydrogels.					
31490664	5	15	theme	encapsulated	948:959	arg1	fibroblasts					966:976	encapsulated L929 fibroblasts	948:976	encapsulated L929 fibroblasts	948:976	Confirming the cytocompatibility of the novel composites, the viability of encapsulated L929 fibroblasts is >90% in all hydrogels.					
31490664	1	16	theme	cellular	206:213	arg1	behavior					215:222	cellular behavior	206:222	cellular behavior	206:222	Naturally, cells reside in three-dimensional (3D) microenvironments composed of biopolymers that guide cellular behavior via topographical features as well as through mechanical and biochemical cues.					
31490664	0	17	theme	Three-Dimensional	11:27	arg1	Microenvironments					29:45	Three-Dimensional Microenvironments	11:45	Three-Dimensional Microenvironments with Starch Nanocrystals as Cell-Instructive Materials	11:100	Engineered Three-Dimensional Microenvironments with Starch Nanocrystals as Cell-Instructive Materials.					
31490664	2	18	theme	topography	353:362	arg1	influence					340:348	the influence	336:348	the influence of topography on cells' behavior	336:381	However, most studies describing the influence of topography on cells' behavior are performed on rigid and synthetic two-dimensional substrates.					
31490664	8	19	theme	potential	1522:1530	arg1	application					1532:1542	a potential application	1520:1542	a potential application of the materials for cartilage tissue engineering	1520:1592	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	2	20	theme	synthetic	410:418	arg1	substrates					436:445	rigid and synthetic two-dimensional substrates	400:445	rigid and synthetic two-dimensional substrates	400:445	However, most studies describing the influence of topography on cells' behavior are performed on rigid and synthetic two-dimensional substrates.					
31490664	6	21	theme	DNA	1040:1042	arg1	content					1044:1050	DNA content	1040:1050	DNA content	1040:1050	The cellular metabolic activity and DNA content are similar for all formulations and increase over time, indicating that the fibroblasts proliferate within the hydrogels.					
31490664	4	22	theme	tunable	857:863	arg1	system					865:870	a tunable system	855:870	a tunable system	855:870	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %) results in an increase of compressive modulus when compared to hydrogels without SNCs, without affecting the swelling ratio, thus providing a tunable system.					
31490664	3	23	theme	gelatin	619:625	arg1	matrix					627:632	a gelatin matrix	617:632	a gelatin matrix	617:632	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	3	24	from	matrix	627:632	arg1	embedded					605:612	embedded	605:612	embedded	605:612	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	8	25	theme	round	1452:1456	arg1	morphology					1458:1467	a round morphology	1450:1467	a round morphology	1450:1467	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	8	26	theme	materials	1551:1559	arg1	application					1532:1542	a potential application	1520:1542	a potential application of the materials for cartilage tissue engineering	1520:1592	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	3	27	theme	embedded	605:612	arg1	SNCs					599:602	SNCs	599:602	SNCs	599:602	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	3	27	theme	embedded	605:612	arg1	nanocrystals					585:596	starch nanocrystals	578:596	starch nanocrystals (SNCs) embedded in a gelatin matrix	578:632	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	1	28	theme	topographical	228:240	arg1	features					242:249	topographical features	228:249	topographical features	228:249	Naturally, cells reside in three-dimensional (3D) microenvironments composed of biopolymers that guide cellular behavior via topographical features as well as through mechanical and biochemical cues.					
31490664	4	29	theme	compressive	741:751	arg1	modulus					753:759	compressive modulus	741:759	compressive modulus	741:759	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %) results in an increase of compressive modulus when compared to hydrogels without SNCs, without affecting the swelling ratio, thus providing a tunable system.					
31490664	8	30	theme	tissue	1575:1580	arg1	engineering					1582:1592	cartilage tissue engineering	1565:1592	cartilage tissue engineering	1565:1592	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	5	31	theme	L929	961:964	arg1	fibroblasts					966:976	encapsulated L929 fibroblasts	948:976	encapsulated L929 fibroblasts	948:976	Confirming the cytocompatibility of the novel composites, the viability of encapsulated L929 fibroblasts is >90% in all hydrogels.					
31490664	1	32	theme	three-dimensional	130:146	arg1	microenvironments					153:169	three-dimensional (3D) microenvironments	130:169	three-dimensional (3D) microenvironments composed of biopolymers that guide cellular behavior via topographical features as well as through mechanical and biochemical cues	130:300	Naturally, cells reside in three-dimensional (3D) microenvironments composed of biopolymers that guide cellular behavior via topographical features as well as through mechanical and biochemical cues.					
31490664	4	33	theme	SNCs	684:687	arg1	concentrations					666:679	different concentrations	656:679	different concentrations of SNCs (0.05, 0.2, and 0.5 wt %)	656:713	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %) results in an increase of compressive modulus when compared to hydrogels without SNCs, without affecting the swelling ratio, thus providing a tunable system.					
31490664	8	34	theme	cartilage	1565:1573	arg1	engineering					1582:1592	cartilage tissue engineering	1565:1592	cartilage tissue engineering	1565:1592	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	7	35	theme	encapsulated	1258:1269	arg1	fibroblasts					1271:1281	the encapsulated fibroblasts	1254:1281	the encapsulated fibroblasts	1254:1281	After 4 d of culture, Live/Dead staining and F-actin/nuclei staining show that the encapsulated fibroblasts develop an elongated morphology in the hydrogels.					
31490664	9	36	theme	topographical	1611:1623	arg1	tool					1691:1694	a powerful tool to instruct cells' behavior	1680:1722	a powerful tool to instruct cells' behavior	1680:1722	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	9	36	theme	topographical	1611:1623	arg1	cues					1640:1643	topographical and mechanical cues	1611:1643	topographical and mechanical cues within 3D microenvironments	1611:1671	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	3	37	theme	native	493:498	arg1	microenvironments					500:516	native microenvironments	493:516	native microenvironments	493:516	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	0	38	theme	Starch	52:57	arg1	Nanocrystals					59:70	Starch Nanocrystals	52:70	Starch Nanocrystals as Cell-Instructive Materials	52:100	Engineered Three-Dimensional Microenvironments with Starch Nanocrystals as Cell-Instructive Materials.					
31490664	4	39	theme	swelling	824:831	arg1	ratio					833:837	the swelling ratio	820:837	the swelling ratio	820:837	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %) results in an increase of compressive modulus when compared to hydrogels without SNCs, without affecting the swelling ratio, thus providing a tunable system.					
31490664	0	40	theme	Cell-Instructive	75:90	arg1	Materials					92:100	Cell-Instructive Materials	75:100	Cell-Instructive Materials	75:100	Engineered Three-Dimensional Microenvironments with Starch Nanocrystals as Cell-Instructive Materials.					
31490664	0	41	with	Microenvironments	29:45	arg1	Nanocrystals					59:70	Starch Nanocrystals	52:70	Starch Nanocrystals as Cell-Instructive Materials	52:100	Engineered Three-Dimensional Microenvironments with Starch Nanocrystals as Cell-Instructive Materials.					
31490664	3	42	theme	3D	537:538	arg1	hydrogels					554:562	3D nanocomposite hydrogels	537:562	3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix	537:632	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	8	43	with	hydrogels	1488:1496	arg1	SNCs					1503:1506	SNCs	1503:1506	SNCs	1503:1506	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	9	44	theme	developed	1737:1745	arg1	nanocomposite					1759:1771	the developed gelatin/SNC nanocomposite	1733:1771	the developed gelatin/SNC nanocomposite	1733:1771	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	9	45	theme	mechanical	1629:1638	arg1	tool					1691:1694	a powerful tool to instruct cells' behavior	1680:1722	a powerful tool to instruct cells' behavior	1680:1722	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	9	45	theme	mechanical	1629:1638	arg1	cues					1640:1643	topographical and mechanical cues	1611:1643	topographical and mechanical cues within 3D microenvironments	1611:1671	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	3	46	theme	nanocomposite	540:552	arg1	hydrogels					554:562	3D nanocomposite hydrogels	537:562	3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix	537:632	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	3	47	from	embedded	605:612	arg1	matrix					627:632	a gelatin matrix	617:632	a gelatin matrix	617:632	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	9	48	theme	gelatin/SNC	1747:1757	arg1	nanocomposite					1759:1771	the developed gelatin/SNC nanocomposite	1733:1771	the developed gelatin/SNC nanocomposite	1733:1771	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	8	49	theme	ATDC5	1389:1393	arg1	cells					1395:1399	encapsulated chondrogenic progenitor ATDC5 cells	1352:1399	encapsulated chondrogenic progenitor ATDC5 cells	1352:1399	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	4	50	theme	wt	709:710	arg1	SNCs					684:687	SNCs	684:687	SNCs (0.05, 0.2, and 0.5 wt %)	684:713	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %) results in an increase of compressive modulus when compared to hydrogels without SNCs, without affecting the swelling ratio, thus providing a tunable system.					
31490664	4	50	theme	wt	709:710	arg1	%					712:712	0.05, 0.2, and 0.5 wt %	690:712	0.05, 0.2, and 0.5 wt %	690:712	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %) results in an increase of compressive modulus when compared to hydrogels without SNCs, without affecting the swelling ratio, thus providing a tunable system.					
31490664	5	51	theme	fibroblasts	966:976	arg1	%					984:984	>90%	981:984	>90% in all hydrogels	981:1001	Confirming the cytocompatibility of the novel composites, the viability of encapsulated L929 fibroblasts is >90% in all hydrogels.					
31490664	5	51	theme	fibroblasts	966:976	arg1	viability					935:943	the viability	931:943	the viability of encapsulated L929 fibroblasts	931:976	Confirming the cytocompatibility of the novel composites, the viability of encapsulated L929 fibroblasts is >90% in all hydrogels.					
31490664	4	52	theme	different	656:664	arg1	concentrations					666:679	different concentrations	656:679	different concentrations of SNCs (0.05, 0.2, and 0.5 wt %)	656:713	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %) results in an increase of compressive modulus when compared to hydrogels without SNCs, without affecting the swelling ratio, thus providing a tunable system.					
31490664	8	53	theme	chondrogenic	1365:1376	arg1	cells					1395:1399	encapsulated chondrogenic progenitor ATDC5 cells	1352:1399	encapsulated chondrogenic progenitor ATDC5 cells	1352:1399	On the other hand, encapsulated chondrogenic progenitor ATDC5 cells also maintain a viability around 90% but display a round morphology, especially in the hydrogels with SNCs, indicating a potential application of the materials for cartilage tissue engineering.					
31490664	1	54	dep	three-dimensional	130:146	arg1	3D					149:150	3D	149:150	3D	149:150	Naturally, cells reside in three-dimensional (3D) microenvironments composed of biopolymers that guide cellular behavior via topographical features as well as through mechanical and biochemical cues.					
31490664	9	55	theme	3D	1652:1653	arg1	microenvironments					1655:1671	3D microenvironments	1652:1671	3D microenvironments	1652:1671	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	2	56	theme	rigid	400:404	arg1	substrates					436:445	rigid and synthetic two-dimensional substrates	400:445	rigid and synthetic two-dimensional substrates	400:445	However, most studies describing the influence of topography on cells' behavior are performed on rigid and synthetic two-dimensional substrates.					
31490664	2	57	theme	two-dimensional	420:434	arg1	substrates					436:445	rigid and synthetic two-dimensional substrates	400:445	rigid and synthetic two-dimensional substrates	400:445	However, most studies describing the influence of topography on cells' behavior are performed on rigid and synthetic two-dimensional substrates.					
31490664	1	58	theme	mechanical	270:279	arg1	cues					297:300	mechanical and biochemical cues	270:300	mechanical and biochemical cues	270:300	Naturally, cells reside in three-dimensional (3D) microenvironments composed of biopolymers that guide cellular behavior via topographical features as well as through mechanical and biochemical cues.					
31490664	9	59	theme	further	1782:1788	arg1	study					1790:1794	further study	1782:1794	further study	1782:1794	We believe that topographical and mechanical cues within 3D microenvironments can be a powerful tool to instruct cells' behavior and that the developed gelatin/SNC nanocomposite warrants further study.					
31490664	6	60	theme	metabolic	1017:1025	arg1	similar					1056:1062	similar	1056:1062	similar	1056:1062	The cellular metabolic activity and DNA content are similar for all formulations and increase over time, indicating that the fibroblasts proliferate within the hydrogels.					
31490664	6	60	theme	metabolic	1017:1025	arg1	activity					1027:1034	The cellular metabolic activity	1004:1034	The cellular metabolic activity	1004:1034	The cellular metabolic activity and DNA content are similar for all formulations and increase over time, indicating that the fibroblasts proliferate within the hydrogels.					
31490664	3	61	theme	starch	578:583	arg1	SNCs					599:602	SNCs	599:602	SNCs	599:602	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	3	61	theme	starch	578:583	arg1	nanocrystals					585:596	starch nanocrystals	578:596	starch nanocrystals (SNCs) embedded in a gelatin matrix	578:632	To design systems that more closely resemble native microenvironments, herein we develop 3D nanocomposite hydrogels consisting of starch nanocrystals (SNCs) embedded in a gelatin matrix.					
31490664	4	62	theme	concentrations	666:679	arg1	incorporation					639:651	The incorporation	635:651	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %)	635:713	The incorporation of different concentrations of SNCs (0.05, 0.2, and 0.5 wt %) results in an increase of compressive modulus when compared to hydrogels without SNCs, without affecting the swelling ratio, thus providing a tunable system.					
29901993	0	0	theme	Films	102:106	arg1	Production					15:24	Singlet Oxygen Production	0:24	Singlet Oxygen Production	0:24	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	0	0	theme	Films	102:106	arg1	Properties					46:55	Tunable Optical Properties	30:55	Tunable Optical Properties	30:55	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	1	1	theme	porphine-5,10,15,20-tetrayl	310:336	arg1	tetrakis					338:345	4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis	303:345	4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP)	303:367	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	1	theme	porphine-5,10,15,20-tetrayl	310:336	arg1	acid					355:358	benzoic acid	347:358	benzoic acid	347:358	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	1	theme	porphine-5,10,15,20-tetrayl	310:336	arg1	mTCPP					362:366	mTCPP	362:366	mTCPP	362:366	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	1	theme	porphine-5,10,15,20-tetrayl	310:336	arg1	4,4					298:300	4,4'	298:301	4,4'	298:301	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	6	2	theme	predictive	1441:1450	arg1	manner					1452:1457	a predictive manner	1439:1457	a predictive manner	1439:1457	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	8	3	theme	optical	1661:1667	arg1	intensities					1711:1721	intensities	1711:1721	intensities	1711:1721	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	8	3	theme	optical	1661:1667	arg1	region					1700:1705	absorption region	1689:1705	absorption region	1689:1705	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	8	3	theme	optical	1661:1667	arg1	properties					1669:1678	optical properties	1661:1678	optical properties	1661:1678	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	3	4	theme	mTCPP	806:810	arg1	groups					796:801	the carboxyl groups	783:801	the carboxyl groups of mTCPP	783:810	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	4	theme	mTCPP	806:810	arg1	mTCPP					806:810	mTCPP	806:810	mTCPP	806:810	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	4	theme	mTCPP	806:810	arg1	DA-chitin					769:777	DA-chitin	769:777	DA-chitin	769:777	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	4	theme	mTCPP	806:810	arg1	groups					759:764	the amino groups	749:764	the amino groups of DA-chitin	749:777	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	5	theme	amino	753:757	arg1	mTCPP					806:810	mTCPP	806:810	mTCPP	806:810	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	5	theme	amino	753:757	arg1	DA-chitin					769:777	DA-chitin	769:777	DA-chitin	769:777	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	5	theme	amino	753:757	arg1	groups					759:764	the amino groups	749:764	the amino groups of DA-chitin	749:777	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	0	6	theme	Crosslinked	90:100	arg1	Films					102:106	Deacetylated Chitin-Porphyrin Crosslinked Films	60:106	Deacetylated Chitin-Porphyrin Crosslinked Films	60:106	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	9	7	from	photosensitizer	1934:1948	arg1	PDT					1953:1955	PDT	1953:1955	PDT	1953:1955	Moreover, the DA-chitin/mTCPP films could generate singlet oxygen under light irradiation and, hence, might serve as a photosensitizer in PDT.					
29901993	2	8	theme	ionic	456:460	arg1	[C2mim					507:512	[C2mim	507:512	[C2mim	507:512	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	2	8	theme	ionic	456:460	arg1	acetate					498:504	the ionic liquid 1-ethyl-3-methyl-imidazolium acetate	452:504	the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc])	452:519	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	1	9	theme	photosensitizer	183:197	arg1	molecules					199:207	photosensitizer molecules	183:207	photosensitizer molecules for photodynamic therapy (PDT)	183:238	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	3	10	theme	carboxyl	787:794	arg1	groups					796:801	the carboxyl groups	783:801	the carboxyl groups of mTCPP	783:810	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	10	theme	carboxyl	787:794	arg1	DA-chitin					769:777	DA-chitin	769:777	DA-chitin	769:777	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	10	theme	carboxyl	787:794	arg1	mTCPP					806:810	mTCPP	806:810	mTCPP	806:810	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	11	theme	deacetylated	633:644	arg1	chitin					646:651	The deacetylated chitin	629:651	The deacetylated chitin (DA-chitin)	629:663	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	11	theme	deacetylated	633:644	arg1	DA-chitin					654:662	DA-chitin	654:662	DA-chitin	654:662	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	2	12	theme	deacetylation	599:611	arg1	degrees					588:594	varying degrees	580:594	varying degrees of deacetylation	580:611	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	8	13	theme	chelating	1639:1647	arg1	ions					1655:1658	chelating metal ions	1639:1658	chelating metal ions	1639:1658	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	6	14	contain	had	1288:1290	arg2	absorbance					1307:1316	strong optical absorbance	1292:1316	strong optical absorbance	1292:1316	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	6	14	contain	had	1288:1290	arg1	films					1282:1286	The DA-chitin/mTCPP films	1262:1286	The DA-chitin/mTCPP films	1262:1286	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	3	15	dep	N-	818:819	arg1	propyl					838:843	propyl	838:843	propyl	838:843	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	9	16	theme	singlet	1866:1872	arg1	oxygen					1874:1879	singlet oxygen	1866:1879	singlet oxygen	1866:1879	Moreover, the DA-chitin/mTCPP films could generate singlet oxygen under light irradiation and, hence, might serve as a photosensitizer in PDT.					
29901993	9	17	theme	DA-chitin/mTCPP	1829:1843	arg1	films					1845:1849	the DA-chitin/mTCPP films	1825:1849	the DA-chitin/mTCPP films	1825:1849	Moreover, the DA-chitin/mTCPP films could generate singlet oxygen under light irradiation and, hence, might serve as a photosensitizer in PDT.					
29901993	1	18	theme	chitin	274:279	arg1	use					267:269	the use	263:269	the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production	263:397	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	19	theme	benzoic	347:353	arg1	tetrakis					338:345	4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis	303:345	4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP)	303:367	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	19	theme	benzoic	347:353	arg1	acid					355:358	benzoic acid	347:358	benzoic acid	347:358	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	2	20	theme	varying	580:586	arg1	degrees					588:594	varying degrees	580:594	varying degrees of deacetylation	580:611	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	4	21	theme	resulting	938:946	arg1	polymers					958:965	The resulting composite polymers	934:965	The resulting composite polymers	934:965	The resulting composite polymers were cast as a film and coagulated with water to remove IL and excess reagents, resulting in homogeneous DA-chitin/mTCPP films.					
29901993	5	22	from	solution	1143:1150	arg1	films					1115:1119	films	1115:1119	films by coagulation from a solution containing chitin	1115:1168	Attempts to prepare films by coagulation from a solution containing chitin and mTCPP to physically entrap the porphyrin, resulted in aggregation of mTCPP in the film.					
29901993	6	23	theme	DA-chitin	1426:1434	arg1	deacetylation					1409:1421	degrees of deacetylation	1398:1421	degrees of deacetylation	1398:1421	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	6	23	theme	DA-chitin	1426:1434	arg1	content					1386:1392	mTCPP content	1380:1392	mTCPP content	1380:1392	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	0	24	theme	Oxygen	8:13	arg1	Production					15:24	Singlet Oxygen Production	0:24	Singlet Oxygen Production	0:24	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	3	25	attach	attached	720:727	arg2	mTCPP					699:703	mTCPP	699:703	mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators	699:931	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	25	attach	attached	720:727	arg3	reaction					732:739	reaction	732:739	reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP	732:810	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	2	26	theme	1-ethyl-3-methyl-imidazolium	469:496	arg1	[C2mim					507:512	[C2mim	507:512	[C2mim	507:512	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	2	26	theme	1-ethyl-3-methyl-imidazolium	469:496	arg1	acetate					498:504	the ionic liquid 1-ethyl-3-methyl-imidazolium acetate	452:504	the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc])	452:519	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	4	27	theme	IL	1023:1024	arg1	reagents					1037:1044	IL and excess reagents	1023:1044	IL and excess reagents	1023:1044	The resulting composite polymers were cast as a film and coagulated with water to remove IL and excess reagents, resulting in homogeneous DA-chitin/mTCPP films.					
29901993	1	28	theme	photodynamic	213:224	arg1	PDT					235:237	PDT	235:237	PDT	235:237	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	28	theme	photodynamic	213:224	arg1	therapy					226:232	photodynamic therapy	213:232	photodynamic therapy (PDT)	213:238	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	0	29	theme	Singlet	0:6	arg1	Production					15:24	Singlet Oxygen Production	0:24	Singlet Oxygen Production	0:24	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	8	30	theme	films	1731:1735	arg1	intensities					1711:1721	intensities	1711:1721	intensities	1711:1721	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	8	30	theme	films	1731:1735	arg1	region					1700:1705	absorption region	1689:1705	absorption region	1689:1705	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	8	30	theme	films	1731:1735	arg1	properties					1669:1678	optical properties	1661:1678	optical properties	1661:1678	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	2	31	theme	M	621:621	arg1	NaOH					623:626	4 M NaOH	619:626	4 M NaOH	619:626	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	8	32	theme	optical	1795:1801	arg1	properties					1803:1812	their optical properties	1789:1812	their optical properties	1789:1812	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	3	33	theme	[OAc	689:692	arg1	[C2mim					682:687	[C2mim	682:687	[C2mim	682:687	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	2	34	theme	4	619:619	arg1	M					621:621	M	621:621	M	621:621	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	1	35	theme	increasing	113:122	arg1	need					124:127	The increasing need	109:127	The increasing need for biocompatible materials	109:155	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	6	36	theme	of	1406:1407	arg1	deacetylation					1409:1421	degrees of deacetylation	1398:1421	degrees of deacetylation	1398:1421	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	10	37	theme	other	2027:2031	arg1	devices					2056:2062	other functional biomaterial devices	2027:2062	other functional biomaterial devices	2027:2062	The methodology used in this study is also applicable for developing other functional biomaterial devices.					
29901993	6	38	theme	optical	1299:1305	arg1	absorbance					1307:1316	strong optical absorbance	1292:1316	strong optical absorbance	1292:1316	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	6	39	theme	degrees	1398:1404	arg1	deacetylation					1409:1421	degrees of deacetylation	1398:1421	degrees of deacetylation	1398:1421	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	0	40	theme	Optical	38:44	arg1	Properties					46:55	Tunable Optical Properties	30:55	Tunable Optical Properties	30:55	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	8	41	theme	metal	1767:1771	arg1	ions					1773:1776	chelating metal ions	1757:1776	chelating metal ions	1757:1776	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	7	42	theme	DA-chitin/mTCPP	1547:1561	arg1	films					1563:1567	the DA-chitin/mTCPP films	1543:1567	the DA-chitin/mTCPP films	1543:1567	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	7	43	theme	salt	1590:1593	arg1	solutions					1595:1603	metal salt solutions	1584:1603	metal salt solutions	1584:1603	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	0	44	theme	Tunable	30:36	arg1	Properties					46:55	Tunable Optical Properties	30:55	Tunable Optical Properties	30:55	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	6	45	theme	DA-chitin/mTCPP	1266:1280	arg1	films					1282:1286	The DA-chitin/mTCPP films	1262:1286	The DA-chitin/mTCPP films	1262:1286	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	2	46	dep	[C2mim	507:512	arg1	[OAc					514:517	[OAc	514:517	[OAc	514:517	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	1	47	dep	led	241:243	arg1	supports					160:167	supports	160:167	supports to immobilize photosensitizer molecules for photodynamic therapy (PDT)	160:238	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	9	48	theme	light	1887:1891	arg1	irradiation					1893:1903	light irradiation	1887:1903	light irradiation	1887:1903	Moreover, the DA-chitin/mTCPP films could generate singlet oxygen under light irradiation and, hence, might serve as a photosensitizer in PDT.					
29901993	3	49	theme	N'-ethylcarbodiimide	847:866	arg1	activators					922:931	activators	922:931	activators	922:931	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	49	theme	N'-ethylcarbodiimide	847:866	arg1	N-hydroxysuccinimide					892:911	N-hydroxysuccinimide	892:911	N-hydroxysuccinimide (NHS)	892:917	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	49	theme	N'-ethylcarbodiimide	847:866	arg1	hydrochloride					868:880	N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride	818:880	hydrochloride	868:880	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	49	theme	N'-ethylcarbodiimide	847:866	arg1	N-					818:819	N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride	818:880	N-	818:819	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	8	50	theme	absorption	1689:1698	arg1	region					1700:1705	absorption region	1689:1705	absorption region	1689:1705	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	2	51	theme	shrimp	432:437	arg1	shells					439:444	shrimp shells	432:444	shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc])	432:519	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	9	52	dep	generate	1857:1864	arg1	serve					1923:1927	serve	1923:1927	might serve as a photosensitizer in PDT	1917:1955	Moreover, the DA-chitin/mTCPP films could generate singlet oxygen under light irradiation and, hence, might serve as a photosensitizer in PDT.					
29901993	2	53	theme	liquid	462:467	arg1	[C2mim					507:512	[C2mim	507:512	[C2mim	507:512	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	2	53	theme	liquid	462:467	arg1	acetate					498:504	the ionic liquid 1-ethyl-3-methyl-imidazolium acetate	452:504	the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc])	452:519	Chitin was first extracted from shrimp shells using the ionic liquid 1-ethyl-3-methyl-imidazolium acetate ([C2mim][OAc]), coagulated as a floc into water, and then deacetylated to varying degrees of deacetylation using 4 M NaOH.					
29901993	0	54	link	Crosslinked	90:100	arg1	Films					102:106	Deacetylated Chitin-Porphyrin Crosslinked Films	60:106	Deacetylated Chitin-Porphyrin Crosslinked Films	60:106	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	6	55	theme	absorbance	1328:1337	arg1	intensity					1339:1347	their absorbance intensity	1322:1347	their absorbance intensity	1322:1347	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	8	56	theme	chelating	1757:1765	arg1	ions					1773:1776	chelating metal ions	1757:1776	chelating metal ions	1757:1776	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	6	57	theme	strong	1292:1297	arg1	absorbance					1307:1316	strong optical absorbance	1292:1316	strong optical absorbance	1292:1316	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	1	58	theme	singlet	373:379	arg1	production					388:397	singlet oxygen production	373:397	singlet oxygen production	373:397	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	59	theme	biocompatible	133:145	arg1	materials					147:155	biocompatible materials	133:155	biocompatible materials	133:155	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	60	theme	4″,4‴-	303:308	arg1	tetrakis					338:345	4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis	303:345	4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP)	303:367	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	60	theme	4″,4‴-	303:308	arg1	acid					355:358	benzoic acid	347:358	benzoic acid	347:358	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	60	theme	4″,4‴-	303:308	arg1	mTCPP					362:366	mTCPP	362:366	mTCPP	362:366	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	60	theme	4″,4‴-	303:308	arg1	4,4					298:300	4,4'	298:301	4,4'	298:301	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	1	61	theme	oxygen	381:386	arg1	production					388:397	singlet oxygen production	373:397	singlet oxygen production	373:397	The increasing need for biocompatible materials as supports to immobilize photosensitizer molecules for photodynamic therapy (PDT), led us to investigate the use of chitin as a support for 4,4',4″,4‴-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (mTCPP) for singlet oxygen production.					
29901993	5	62	from	aggregation	1228:1238	arg1	film					1256:1259	the film	1252:1259	the film	1252:1259	Attempts to prepare films by coagulation from a solution containing chitin and mTCPP to physically entrap the porphyrin, resulted in aggregation of mTCPP in the film.					
29901993	4	63	theme	DA-chitin/mTCPP	1072:1086	arg1	films					1088:1092	homogeneous DA-chitin/mTCPP films	1060:1092	homogeneous DA-chitin/mTCPP films	1060:1092	The resulting composite polymers were cast as a film and coagulated with water to remove IL and excess reagents, resulting in homogeneous DA-chitin/mTCPP films.					
29901993	7	64	dep	ions	1479:1482	arg1	Gd3+					1497:1500	Gd3+	1497:1500	Gd3+	1497:1500	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	7	64	dep	ions	1479:1482	arg1	Fe3+					1507:1510	Fe3+	1507:1510	Fe3+	1507:1510	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	7	64	dep	ions	1479:1482	arg1	Zn2+					1491:1494	Zn2+	1491:1494	Zn2+	1491:1494	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	7	64	dep	ions	1479:1482	arg1	Cu2+					1485:1488	Cu2+	1485:1488	Cu2+	1485:1488	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	7	64	dep	ions	1479:1482	arg1	ions					1479:1482	metal ions	1473:1482	metal ions (Cu2+, Zn2+, Gd3+, and Fe3+)	1473:1511	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	6	65	theme	mTCPP	1380:1384	arg1	content					1386:1392	mTCPP content	1380:1392	mTCPP content	1380:1392	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	7	66	theme	metal	1473:1477	arg1	Fe3+					1507:1510	Fe3+	1507:1510	Fe3+	1507:1510	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	7	66	theme	metal	1473:1477	arg1	Zn2+					1491:1494	Zn2+	1491:1494	Zn2+	1491:1494	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	7	66	theme	metal	1473:1477	arg1	Cu2+					1485:1488	Cu2+	1485:1488	Cu2+	1485:1488	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	7	66	theme	metal	1473:1477	arg1	ions					1479:1482	metal ions	1473:1482	metal ions (Cu2+, Zn2+, Gd3+, and Fe3+)	1473:1511	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	8	67	theme	metal	1649:1653	arg1	ions					1655:1658	chelating metal ions	1639:1658	chelating metal ions	1639:1658	After chelating metal ions, optical properties, such as absorption region and intensities, of the films changed, suggesting chelating metal ions could tune their optical properties.					
29901993	4	68	theme	homogeneous	1060:1070	arg1	films					1088:1092	homogeneous DA-chitin/mTCPP films	1060:1092	homogeneous DA-chitin/mTCPP films	1060:1092	The resulting composite polymers were cast as a film and coagulated with water to remove IL and excess reagents, resulting in homogeneous DA-chitin/mTCPP films.					
29901993	5	69	theme	mTCPP	1243:1247	arg1	aggregation					1228:1238	aggregation	1228:1238	aggregation of mTCPP in the film	1228:1259	Attempts to prepare films by coagulation from a solution containing chitin and mTCPP to physically entrap the porphyrin, resulted in aggregation of mTCPP in the film.					
29901993	7	70	theme	metal	1584:1588	arg1	solutions					1595:1603	metal salt solutions	1584:1603	metal salt solutions	1584:1603	In addition, metal ions (Cu2+, Zn2+, Gd3+, and Fe3+) could be easily chelated into the DA-chitin/mTCPP films through mixing metal salt solutions with the films and heating.					
29901993	10	71	theme	biomaterial	2044:2054	arg1	devices					2056:2062	other functional biomaterial devices	2027:2062	other functional biomaterial devices	2027:2062	The methodology used in this study is also applicable for developing other functional biomaterial devices.					
29901993	0	72	theme	Chitin-Porphyrin	73:88	arg1	Films					102:106	Deacetylated Chitin-Porphyrin Crosslinked Films	60:106	Deacetylated Chitin-Porphyrin Crosslinked Films	60:106	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	5	73	dep	Attempts	1095:1102	arg1	resulted					1216:1223	resulted	1216:1223	resulted in aggregation of mTCPP in the film	1216:1259	Attempts to prepare films by coagulation from a solution containing chitin and mTCPP to physically entrap the porphyrin, resulted in aggregation of mTCPP in the film.					
29901993	4	74	theme	excess	1030:1035	arg1	reagents					1037:1044	IL and excess reagents	1023:1044	IL and excess reagents	1023:1044	The resulting composite polymers were cast as a film and coagulated with water to remove IL and excess reagents, resulting in homogeneous DA-chitin/mTCPP films.					
29901993	3	75	theme	DA-chitin	769:777	arg1	groups					796:801	the carboxyl groups	783:801	the carboxyl groups of mTCPP	783:810	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	75	theme	DA-chitin	769:777	arg1	mTCPP					806:810	mTCPP	806:810	mTCPP	806:810	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	75	theme	DA-chitin	769:777	arg1	DA-chitin					769:777	DA-chitin	769:777	DA-chitin	769:777	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	3	75	theme	DA-chitin	769:777	arg1	groups					759:764	the amino groups	749:764	the amino groups of DA-chitin	749:777	The deacetylated chitin (DA-chitin) was dissolved in [C2mim][OAc] and mTCPP was covalently attached by reaction between the amino groups of DA-chitin and the carboxyl groups of mTCPP using N-(3-(dimethylamino)propyl)- N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) as activators.					
29901993	0	76	theme	Deacetylated	60:71	arg1	Films					102:106	Deacetylated Chitin-Porphyrin Crosslinked Films	60:106	Deacetylated Chitin-Porphyrin Crosslinked Films	60:106	Singlet Oxygen Production and Tunable Optical Properties of Deacetylated Chitin-Porphyrin Crosslinked Films.					
29901993	10	77	theme	functional	2033:2042	arg1	devices					2056:2062	other functional biomaterial devices	2027:2062	other functional biomaterial devices	2027:2062	The methodology used in this study is also applicable for developing other functional biomaterial devices.					
29901993	6	78	dep	content	1386:1392	arg1	the					1376:1378	the	1376:1378	the	1376:1378	The DA-chitin/mTCPP films had strong optical absorbance and their absorbance intensity could be tuned by changing the mTCPP content and degrees of deacetylation of DA-chitin in a predictive manner.					
29901993	4	79	theme	composite	948:956	arg1	polymers					958:965	The resulting composite polymers	934:965	The resulting composite polymers	934:965	The resulting composite polymers were cast as a film and coagulated with water to remove IL and excess reagents, resulting in homogeneous DA-chitin/mTCPP films.					
29901993	5	80	contain	containing	1152:1161	arg2	chitin					1163:1168	chitin	1163:1168	chitin	1163:1168	Attempts to prepare films by coagulation from a solution containing chitin and mTCPP to physically entrap the porphyrin, resulted in aggregation of mTCPP in the film.					
29901993	5	80	contain	containing	1152:1161	arg1	solution					1143:1150	a solution	1141:1150	a solution containing chitin	1141:1168	Attempts to prepare films by coagulation from a solution containing chitin and mTCPP to physically entrap the porphyrin, resulted in aggregation of mTCPP in the film.					
32059880	9	0	theme	PCL/Gel/BCNC	1422:1433	arg1	scaffolds					1445:1453	PCL/Gel/BCNC composite scaffolds	1422:1453	PCL/Gel/BCNC composite scaffolds	1422:1453	Based on these results, PCL/Gel/BCNC composite scaffolds are a good candidate for biomimetic GBM tumor platform.					
32059880	9	0	theme	PCL/Gel/BCNC	1422:1433	arg1	candidate					1466:1474	a good candidate	1459:1474	a good candidate for biomimetic GBM tumor platform	1459:1508	Based on these results, PCL/Gel/BCNC composite scaffolds are a good candidate for biomimetic GBM tumor platform.					
32059880	2	1	theme	cell	340:343	arg1	culture					345:351	standard two-dimensional (2D) cell culture	310:351	standard two-dimensional (2D) cell culture	310:351	In the last decades, it is indicated that standard two-dimensional (2D) cell culture is inadequate to improve new therapeutic strategies and drug development.					
32059880	1	2	theme	recurrence	250:259	arg1	rates					261:265	high recurrence rates	245:265	high recurrence rates	245:265	Glioblastoma (GBM), the most common and extremely lethal type of brain tumor, is resistant to treatment and shows high recurrence rates.					
32059880	0	3	theme	electrospun	107:117	arg1	nanofibers					119:128	polycaprolactone/gelatin electrospun nanofibers	82:128	polycaprolactone/gelatin electrospun nanofibers	82:128	Glioblastoma cell adhesion properties through bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers.					
32059880	1	4	theme	lethal	181:186	arg1	Glioblastoma					131:142	Glioblastoma	131:142	Glioblastoma (GBM)	131:148	Glioblastoma (GBM), the most common and extremely lethal type of brain tumor, is resistant to treatment and shows high recurrence rates.					
32059880	1	4	theme	lethal	181:186	arg1	type					188:191	the most common and extremely lethal type	151:191	the most common and extremely lethal type of brain tumor	151:206	Glioblastoma (GBM), the most common and extremely lethal type of brain tumor, is resistant to treatment and shows high recurrence rates.					
32059880	5	5	theme	increased	866:874	arg1	concentration					876:888	an increased concentration	863:888	an increased concentration of BCNC	863:896	The fiber diameters in the nanofibrous matrix were increased with an increased concentration of BCNC.					
32059880	4	6	theme	extracellular	762:774	arg1	matrix					776:781	the extracellular matrix	758:781	the extracellular matrix of GBM tumor	758:794	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	7	7	theme	U251 MG	1110:1116	arg1	cells					1131:1135	U251 MG glioblastoma cells	1110:1135	U251 MG glioblastoma cells	1110:1135	In-vitro biocompatibilities of nanofibrous scaffolds were tested with U251 MG glioblastoma cells and improved cell adhesion and proliferation was compared with PCL/Gel.					
32059880	3	8	theme	in	524:525	arg1	models					552:557	in vitro and in vivo cancer models	524:557	in vitro and in vivo cancer models	524:557	Hence, well-mimicked three-dimensional (3D) tumor platforms are needed to bridge the gap between in vitro and in vivo cancer models.					
32059880	6	9	theme	beaded	967:972	arg1	formation					974:982	the beaded formation	963:982	the beaded formation	963:982	Moreover, fiber morphology changed from the smooth formation to the beaded formation by increasing the concentration of the BCNC suspension.					
32059880	0	10	from	nanocrystals	66:77	arg1	nanofibers					119:128	polycaprolactone/gelatin electrospun nanofibers	82:128	polycaprolactone/gelatin electrospun nanofibers	82:128	Glioblastoma cell adhesion properties through bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers.					
32059880	7	11	theme	glioblastoma	1118:1129	arg1	cells					1131:1135	U251 MG glioblastoma cells	1110:1135	U251 MG glioblastoma cells	1110:1135	In-vitro biocompatibilities of nanofibrous scaffolds were tested with U251 MG glioblastoma cells and improved cell adhesion and proliferation was compared with PCL/Gel.					
32059880	3	12	theme	tumor	471:475	arg1	platforms					477:485	well-mimicked three-dimensional (3D) tumor platforms	434:485	well-mimicked three-dimensional (3D) tumor platforms	434:485	Hence, well-mimicked three-dimensional (3D) tumor platforms are needed to bridge the gap between in vitro and in vivo cancer models.					
32059880	5	13	theme	BCNC	893:896	arg1	concentration					876:888	an increased concentration	863:888	an increased concentration of BCNC	863:896	The fiber diameters in the nanofibrous matrix were increased with an increased concentration of BCNC.					
32059880	2	14	theme	last	275:278	arg1	decades					280:286	the last decades	271:286	the last decades	271:286	In the last decades, it is indicated that standard two-dimensional (2D) cell culture is inadequate to improve new therapeutic strategies and drug development.					
32059880	9	15	theme	GBM	1491:1493	arg1	platform					1501:1508	biomimetic GBM tumor platform	1480:1508	biomimetic GBM tumor platform	1480:1508	Based on these results, PCL/Gel/BCNC composite scaffolds are a good candidate for biomimetic GBM tumor platform.					
32059880	2	16	theme	new	378:380	arg1	strategies					394:403	new therapeutic strategies	378:403	new therapeutic strategies	378:403	In the last decades, it is indicated that standard two-dimensional (2D) cell culture is inadequate to improve new therapeutic strategies and drug development.					
32059880	7	17	theme	cell	1150:1153	arg1	adhesion					1155:1162	improved cell adhesion	1141:1162	improved cell adhesion	1141:1162	In-vitro biocompatibilities of nanofibrous scaffolds were tested with U251 MG glioblastoma cells and improved cell adhesion and proliferation was compared with PCL/Gel.					
32059880	8	18	theme	recurrence	1386:1395	arg1	process					1369:1375	the process	1365:1375	the process of tumor recurrence	1365:1395	PCL/Gel/BCNC were found suitable for enhancing axon growth and elongation supporting communication between tumor cells and the microenvironment, triggering the process of tumor recurrence.					
32059880	8	19	theme	axon	1256:1259	arg1	growth					1261:1266	axon growth	1256:1266	axon growth	1256:1266	PCL/Gel/BCNC were found suitable for enhancing axon growth and elongation supporting communication between tumor cells and the microenvironment, triggering the process of tumor recurrence.					
32059880	0	20	theme	cell	13:16	arg1	properties					27:36	Glioblastoma cell adhesion properties	0:36	Glioblastoma cell adhesion properties	0:36	Glioblastoma cell adhesion properties through bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers.					
32059880	7	21	theme	scaffolds	1083:1091	arg1	biocompatibilities					1049:1066	In-vitro biocompatibilities	1040:1066	In-vitro biocompatibilities of nanofibrous scaffolds	1040:1091	In-vitro biocompatibilities of nanofibrous scaffolds were tested with U251 MG glioblastoma cells and improved cell adhesion and proliferation was compared with PCL/Gel.					
32059880	2	22	dep	two-dimensional	319:333	arg1	2D					336:337	2D	336:337	2D	336:337	In the last decades, it is indicated that standard two-dimensional (2D) cell culture is inadequate to improve new therapeutic strategies and drug development.					
32059880	1	23	theme	brain	196:200	arg1	tumor					202:206	brain tumor	196:206	brain tumor	196:206	Glioblastoma (GBM), the most common and extremely lethal type of brain tumor, is resistant to treatment and shows high recurrence rates.					
32059880	0	24	theme	Glioblastoma	0:11	arg1	properties					27:36	Glioblastoma cell adhesion properties	0:36	Glioblastoma cell adhesion properties	0:36	Glioblastoma cell adhesion properties through bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers.					
32059880	8	25	theme	tumor	1380:1384	arg1	recurrence					1386:1395	tumor recurrence	1380:1395	tumor recurrence	1380:1395	PCL/Gel/BCNC were found suitable for enhancing axon growth and elongation supporting communication between tumor cells and the microenvironment, triggering the process of tumor recurrence.					
32059880	4	26	theme	composite	676:684	arg1	scaffolds					686:694	polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds	626:694	polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds	626:694	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	3	27	theme	cancer	545:550	arg1	models					552:557	in vitro and in vivo cancer models	524:557	in vitro and in vivo cancer models	524:557	Hence, well-mimicked three-dimensional (3D) tumor platforms are needed to bridge the gap between in vitro and in vivo cancer models.					
32059880	1	28	theme	tumor	202:206	arg1	Glioblastoma					131:142	Glioblastoma	131:142	Glioblastoma (GBM)	131:148	Glioblastoma (GBM), the most common and extremely lethal type of brain tumor, is resistant to treatment and shows high recurrence rates.					
32059880	1	28	theme	tumor	202:206	arg1	type					188:191	the most common and extremely lethal type	151:191	the most common and extremely lethal type of brain tumor	151:206	Glioblastoma (GBM), the most common and extremely lethal type of brain tumor, is resistant to treatment and shows high recurrence rates.					
32059880	2	29	theme	drug	409:412	arg1	development					414:424	drug development	409:424	drug development	409:424	In the last decades, it is indicated that standard two-dimensional (2D) cell culture is inadequate to improve new therapeutic strategies and drug development.					
32059880	0	30	theme	adhesion	18:25	arg1	properties					27:36	Glioblastoma cell adhesion properties	0:36	Glioblastoma cell adhesion properties	0:36	Glioblastoma cell adhesion properties through bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers.					
32059880	6	31	theme	smooth	943:948	arg1	formation					950:958	the smooth formation	939:958	the smooth formation	939:958	Moreover, fiber morphology changed from the smooth formation to the beaded formation by increasing the concentration of the BCNC suspension.					
32059880	7	32	theme	nanofibrous	1071:1081	arg1	scaffolds					1083:1091	nanofibrous scaffolds	1071:1091	nanofibrous scaffolds	1071:1091	In-vitro biocompatibilities of nanofibrous scaffolds were tested with U251 MG glioblastoma cells and improved cell adhesion and proliferation was compared with PCL/Gel.					
32059880	3	33	theme	in	537:538	arg1	models					552:557	in vitro and in vivo cancer models	524:557	in vitro and in vivo cancer models	524:557	Hence, well-mimicked three-dimensional (3D) tumor platforms are needed to bridge the gap between in vitro and in vivo cancer models.					
32059880	6	34	theme	suspension	1028:1037	arg1	concentration					1002:1014	the concentration	998:1014	the concentration of the BCNC suspension	998:1037	Moreover, fiber morphology changed from the smooth formation to the beaded formation by increasing the concentration of the BCNC suspension.					
32059880	0	35	theme	bacterial	46:54	arg1	nanocrystals					66:77	bacterial cellulose nanocrystals	46:77	bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers	46:128	Glioblastoma cell adhesion properties through bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers.					
32059880	7	36	theme	In-vitro	1040:1047	arg1	biocompatibilities					1049:1066	In-vitro biocompatibilities	1040:1066	In-vitro biocompatibilities of nanofibrous scaffolds	1040:1091	In-vitro biocompatibilities of nanofibrous scaffolds were tested with U251 MG glioblastoma cells and improved cell adhesion and proliferation was compared with PCL/Gel.					
32059880	6	37	theme	BCNC	1023:1026	arg1	suspension					1028:1037	the BCNC suspension	1019:1037	the BCNC suspension	1019:1037	Moreover, fiber morphology changed from the smooth formation to the beaded formation by increasing the concentration of the BCNC suspension.					
32059880	9	38	theme	tumor	1495:1499	arg1	platform					1501:1508	biomimetic GBM tumor platform	1480:1508	biomimetic GBM tumor platform	1480:1508	Based on these results, PCL/Gel/BCNC composite scaffolds are a good candidate for biomimetic GBM tumor platform.					
32059880	3	39	theme	well-mimicked	434:446	arg1	platforms					477:485	well-mimicked three-dimensional (3D) tumor platforms	434:485	well-mimicked three-dimensional (3D) tumor platforms	434:485	Hence, well-mimicked three-dimensional (3D) tumor platforms are needed to bridge the gap between in vitro and in vivo cancer models.					
32059880	8	40	theme	tumor	1316:1320	arg1	cells					1322:1326	tumor cells	1316:1326	tumor cells	1316:1326	PCL/Gel/BCNC were found suitable for enhancing axon growth and elongation supporting communication between tumor cells and the microenvironment, triggering the process of tumor recurrence.					
32059880	4	41	theme	tumor	790:794	arg1	matrix					776:781	the extracellular matrix	758:781	the extracellular matrix of GBM tumor	758:794	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	5	42	theme	nanofibrous	824:834	arg1	matrix					836:841	the nanofibrous matrix	820:841	the nanofibrous matrix	820:841	The fiber diameters in the nanofibrous matrix were increased with an increased concentration of BCNC.					
32059880	2	43	theme	therapeutic	382:392	arg1	strategies					394:403	new therapeutic strategies	378:403	new therapeutic strategies	378:403	In the last decades, it is indicated that standard two-dimensional (2D) cell culture is inadequate to improve new therapeutic strategies and drug development.					
32059880	7	44	theme	improved	1141:1148	arg1	adhesion					1155:1162	improved cell adhesion	1141:1162	improved cell adhesion	1141:1162	In-vitro biocompatibilities of nanofibrous scaffolds were tested with U251 MG glioblastoma cells and improved cell adhesion and proliferation was compared with PCL/Gel.					
32059880	5	45	theme	fiber	801:805	arg1	diameters					807:815	The fiber diameters	797:815	The fiber diameters in the nanofibrous matrix	797:841	The fiber diameters in the nanofibrous matrix were increased with an increased concentration of BCNC.					
32059880	4	46	theme	nanofibrous	664:674	arg1	scaffolds					686:694	polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds	626:694	polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds	626:694	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	0	47	theme	cellulose	56:64	arg1	nanocrystals					66:77	bacterial cellulose nanocrystals	46:77	bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers	46:128	Glioblastoma cell adhesion properties through bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers.					
32059880	5	48	from	diameters	807:815	arg1	matrix					836:841	the nanofibrous matrix	820:841	the nanofibrous matrix	820:841	The fiber diameters in the nanofibrous matrix were increased with an increased concentration of BCNC.					
32059880	6	49	theme	fiber	909:913	arg1	morphology					915:924	fiber morphology	909:924	fiber morphology	909:924	Moreover, fiber morphology changed from the smooth formation to the beaded formation by increasing the concentration of the BCNC suspension.					
32059880	0	50	dep	properties	27:36	arg1	nanocrystals					66:77	bacterial cellulose nanocrystals	46:77	bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers	46:128	Glioblastoma cell adhesion properties through bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers.					
32059880	2	51	theme	two-dimensional	319:333	arg1	culture					345:351	standard two-dimensional (2D) cell culture	310:351	standard two-dimensional (2D) cell culture	310:351	In the last decades, it is indicated that standard two-dimensional (2D) cell culture is inadequate to improve new therapeutic strategies and drug development.					
32059880	4	52	contain	containing	615:624	arg2	scaffolds					686:694	polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds	626:694	polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds	626:694	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	4	52	contain	containing	615:624	arg1	BCNC					609:612	BCNC	609:612	BCNC	609:612	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	4	52	contain	containing	615:624	arg1	nano-crystal					595:606	bacterial cellulose nano-crystal	575:606	bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds	575:694	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	3	53	dep	three-dimensional	448:464	arg1	3D					467:468	3D	467:468	3D	467:468	Hence, well-mimicked three-dimensional (3D) tumor platforms are needed to bridge the gap between in vitro and in vivo cancer models.					
32059880	9	54	theme	composite	1435:1443	arg1	scaffolds					1445:1453	PCL/Gel/BCNC composite scaffolds	1422:1453	PCL/Gel/BCNC composite scaffolds	1422:1453	Based on these results, PCL/Gel/BCNC composite scaffolds are a good candidate for biomimetic GBM tumor platform.					
32059880	9	54	theme	composite	1435:1443	arg1	candidate					1466:1474	a good candidate	1459:1474	a good candidate for biomimetic GBM tumor platform	1459:1508	Based on these results, PCL/Gel/BCNC composite scaffolds are a good candidate for biomimetic GBM tumor platform.					
32059880	2	55	theme	standard	310:317	arg1	culture					345:351	standard two-dimensional (2D) cell culture	310:351	standard two-dimensional (2D) cell culture	310:351	In the last decades, it is indicated that standard two-dimensional (2D) cell culture is inadequate to improve new therapeutic strategies and drug development.					
32059880	3	56	dep	in	524:525	arg1	vitro					527:531	vitro	527:531	vitro	527:531	Hence, well-mimicked three-dimensional (3D) tumor platforms are needed to bridge the gap between in vitro and in vivo cancer models.					
32059880	0	57	theme	polycaprolactone/gelatin	82:105	arg1	nanofibers					119:128	polycaprolactone/gelatin electrospun nanofibers	82:128	polycaprolactone/gelatin electrospun nanofibers	82:128	Glioblastoma cell adhesion properties through bacterial cellulose nanocrystals in polycaprolactone/gelatin electrospun nanofibers.					
32059880	3	58	theme	three-dimensional	448:464	arg1	platforms					477:485	well-mimicked three-dimensional (3D) tumor platforms	434:485	well-mimicked three-dimensional (3D) tumor platforms	434:485	Hence, well-mimicked three-dimensional (3D) tumor platforms are needed to bridge the gap between in vitro and in vivo cancer models.					
32059880	4	59	theme	GBM	786:788	arg1	tumor					790:794	GBM tumor	786:794	GBM tumor	786:794	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	1	60	theme	common	160:165	arg1	Glioblastoma					131:142	Glioblastoma	131:142	Glioblastoma (GBM)	131:148	Glioblastoma (GBM), the most common and extremely lethal type of brain tumor, is resistant to treatment and shows high recurrence rates.					
32059880	1	60	theme	common	160:165	arg1	type					188:191	the most common and extremely lethal type	151:191	the most common and extremely lethal type of brain tumor	151:206	Glioblastoma (GBM), the most common and extremely lethal type of brain tumor, is resistant to treatment and shows high recurrence rates.					
32059880	9	61	theme	biomimetic	1480:1489	arg1	platform					1501:1508	biomimetic GBM tumor platform	1480:1508	biomimetic GBM tumor platform	1480:1508	Based on these results, PCL/Gel/BCNC composite scaffolds are a good candidate for biomimetic GBM tumor platform.					
32059880	4	62	theme	cellulose	585:593	arg1	BCNC					609:612	BCNC	609:612	BCNC	609:612	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	4	62	theme	cellulose	585:593	arg1	nano-crystal					595:606	bacterial cellulose nano-crystal	575:606	bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds	575:694	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	9	63	theme	good	1461:1464	arg1	scaffolds					1445:1453	PCL/Gel/BCNC composite scaffolds	1422:1453	PCL/Gel/BCNC composite scaffolds	1422:1453	Based on these results, PCL/Gel/BCNC composite scaffolds are a good candidate for biomimetic GBM tumor platform.					
32059880	9	63	theme	good	1461:1464	arg1	candidate					1466:1474	a good candidate	1459:1474	a good candidate for biomimetic GBM tumor platform	1459:1508	Based on these results, PCL/Gel/BCNC composite scaffolds are a good candidate for biomimetic GBM tumor platform.					
32059880	4	64	theme	bacterial	575:583	arg1	BCNC					609:612	BCNC	609:612	BCNC	609:612	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	4	64	theme	bacterial	575:583	arg1	nano-crystal					595:606	bacterial cellulose nano-crystal	575:606	bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds	575:694	In this study, bacterial cellulose nano-crystal (BCNC) containing polycaprolactone (PCL) /gelatin (Gel) nanofibrous composite scaffolds were successfully fabricated by electrospinning for mimicking the extracellular matrix of GBM tumor.					
32059880	3	65	dep	in	537:538	arg1	vivo					540:543	vivo	540:543	vivo	540:543	Hence, well-mimicked three-dimensional (3D) tumor platforms are needed to bridge the gap between in vitro and in vivo cancer models.					
32059880	1	66	theme	high	245:248	arg1	rates					261:265	high recurrence rates	245:265	high recurrence rates	245:265	Glioblastoma (GBM), the most common and extremely lethal type of brain tumor, is resistant to treatment and shows high recurrence rates.					
30813047	6	0	theme	toxicity	1319:1326	arg1	experiments					1289:1299	The experiments	1285:1299	The experiments of cell and acute toxicity in vivo	1285:1334	The experiments of cell and acute toxicity in vivo showed that GO and its composite hydrogel had good biocompatibility.					
30813047	3	1	theme	squeezing	831:839	arg1	action					841:846	the squeezing action	827:846	the squeezing action	827:846	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	5	2	theme	molecule	1133:1140	arg1	GP					1142:1143	small molecule GP	1127:1143	small molecule GP	1127:1143	GO, PEG and small molecule GP in the slow-release solution through hydrogen-bond interaction might form a particular structure, which led to the good lubricating effect.					
30813047	4	3	theme	experimental	921:932	arg1	results					934:940	The friction experimental results	908:940	The friction experimental results	908:940	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	2	4	theme	joints	566:571	arg1	lubrication					540:550	the lubrication	536:550	the lubrication of artificial joints	536:571	However, the composite hydrogel with slow-release lubrication effect provides a new way for the lubrication of artificial joints.					
30813047	4	5	theme	friction	1008:1015	arg1	coefficient					1017:1027	the average friction coefficient	996:1027	the average friction coefficient of the slow-release solution	996:1056	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	0	6	theme	glycerophosphate	99:114	arg1	hydrogel					116:123	chitosan/sodium glycerophosphate hydrogel	83:123	chitosan/sodium glycerophosphate hydrogel applied on artificial joints	83:152	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	2	7	with	hydrogel	467:474	arg1	effect					506:511	slow-release lubrication effect	481:511	slow-release lubrication effect	481:511	However, the composite hydrogel with slow-release lubrication effect provides a new way for the lubrication of artificial joints.					
30813047	2	8	theme	artificial	555:564	arg1	joints					566:571	artificial joints	555:571	artificial joints	555:571	However, the composite hydrogel with slow-release lubrication effect provides a new way for the lubrication of artificial joints.					
30813047	4	9	contain	had	961:963	arg1	GO/PEG					954:959	GO/PEG	954:959	GO/PEG	954:959	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	4	9	contain	had	961:963	arg2	effect					984:989	better lubrication effect	965:989	better lubrication effect	965:989	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	4	10	theme	average	1000:1006	arg1	coefficient					1017:1027	the average friction coefficient	996:1027	the average friction coefficient of the slow-release solution	996:1056	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	1	11	theme	body	269:272	arg1	fluid					274:278	the body fluid	265:278	the body fluid	265:278	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	0	12	theme	chitosan/sodium	83:97	arg1	hydrogel					116:123	chitosan/sodium glycerophosphate hydrogel	83:123	chitosan/sodium glycerophosphate hydrogel applied on artificial joints	83:152	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	4	13	theme	lubrication	972:982	arg1	effect					984:989	better lubrication effect	965:989	better lubrication effect	965:989	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	5	14	theme	lubricating	1265:1275	arg1	effect					1277:1282	the good lubricating effect	1256:1282	the good lubricating effect	1256:1282	GO, PEG and small molecule GP in the slow-release solution through hydrogen-bond interaction might form a particular structure, which led to the good lubricating effect.					
30813047	1	15	theme	fluid	274:278	arg1	lubrication					250:260	the poor lubrication	241:260	the poor lubrication of the body fluid	241:278	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	3	16	theme	artificial	675:684	arg1	lubrication					692:702	the artificial joint lubrication	671:702	the artificial joint lubrication	671:702	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	4	17	theme	better	965:970	arg1	effect					984:989	better lubrication effect	965:989	better lubrication effect	965:989	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	0	18	theme	lubrication	13:23	arg1	effect					25:30	Slow-release lubrication effect	0:30	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol)	0:70	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	1	19	theme	human	364:368	arg1	tissues					370:376	human tissues	364:376	human tissues	364:376	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	1	20	theme	lubrication	424:434	arg1	effect					436:441	its lubrication effect	420:441	its lubrication effect	420:441	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	1	21	theme	serious	194:200	arg1	surgery					226:232	serious wear after implantation surgery	194:232	serious wear after implantation surgery due to the poor lubrication of the body fluid	194:278	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	0	22	theme	Slow-release	0:11	arg1	effect					25:30	Slow-release lubrication effect	0:30	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol)	0:70	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	3	23	theme	service	869:875	arg1	time					877:880	the service time	865:880	the service time of biomimetic lubricants	865:905	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	6	24	theme	composite	1359:1367	arg1	hydrogel					1369:1376	its composite hydrogel	1355:1376	its composite hydrogel	1355:1376	The experiments of cell and acute toxicity in vivo showed that GO and its composite hydrogel had good biocompatibility.					
30813047	2	25	theme	new	524:526	arg1	way					528:530	a new way	522:530	a new way for the lubrication of artificial joints	522:571	However, the composite hydrogel with slow-release lubrication effect provides a new way for the lubrication of artificial joints.					
30813047	6	26	theme	acute	1313:1317	arg1	toxicity					1319:1326	acute toxicity	1313:1326	acute toxicity	1313:1326	The experiments of cell and acute toxicity in vivo showed that GO and its composite hydrogel had good biocompatibility.					
30813047	4	27	theme	solution	1049:1056	arg1	coefficient					1017:1027	the average friction coefficient	996:1027	the average friction coefficient of the slow-release solution	996:1056	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	3	28	theme	joint	686:690	arg1	lubrication					692:702	the artificial joint lubrication	671:702	the artificial joint lubrication	671:702	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	6	29	theme	cell	1304:1307	arg1	experiments					1289:1299	The experiments	1285:1299	The experiments of cell and acute toxicity in vivo	1285:1334	The experiments of cell and acute toxicity in vivo showed that GO and its composite hydrogel had good biocompatibility.					
30813047	4	30	theme	friction	912:919	arg1	results					934:940	The friction experimental results	908:940	The friction experimental results	908:940	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	0	31	theme	oxide/poly	44:53	arg1	effect					25:30	Slow-release lubrication effect	0:30	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol)	0:70	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	1	32	theme	biomimetic	289:298	arg1	easy					341:344	easy	341:344	easy	341:344	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	1	32	theme	biomimetic	289:298	arg1	lubricants					300:309	the biomimetic lubricants	285:309	the biomimetic lubricants directly injected in vitro	285:336	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	6	33	theme	good	1382:1385	arg1	biocompatibility					1387:1402	good biocompatibility	1382:1402	good biocompatibility	1382:1402	The experiments of cell and acute toxicity in vivo showed that GO and its composite hydrogel had good biocompatibility.					
30813047	3	34	theme	ethylene	609:616	arg1	oxide/Poly					598:607	Graphene oxide/Poly	589:607	Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites	589:644	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	3	34	theme	ethylene	609:616	arg1	glycol					618:623	ethylene glycol	609:623	ethylene glycol	609:623	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	1	35	dep	serious	194:200	arg1	wear					202:205	wear	202:205	wear	202:205	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	5	36	theme	good	1260:1263	arg1	effect					1277:1282	the good lubricating effect	1256:1282	the good lubricating effect	1256:1282	GO, PEG and small molecule GP in the slow-release solution through hydrogen-bond interaction might form a particular structure, which led to the good lubricating effect.					
30813047	0	37	theme	graphene	35:42	arg1	oxide/poly					44:53	graphene oxide/poly	35:53	graphene oxide/poly(ethylene glycol)	35:70	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	0	37	theme	graphene	35:42	arg1	glycol					64:69	ethylene glycol	55:69	ethylene glycol	55:69	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	3	38	theme	Graphene	589:596	arg1	oxide/Poly					598:607	Graphene oxide/Poly	589:607	Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites	589:644	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	3	38	theme	Graphene	589:596	arg1	glycol					618:623	ethylene glycol	609:623	ethylene glycol	609:623	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	3	38	theme	Graphene	589:596	arg1	GO/PEG					627:632	GO/PEG	627:632	GO/PEG	627:632	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	3	39	theme	Chitosan/Sodium	732:746	arg1	hydrogel					773:780	the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel	728:780	the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel	728:780	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	3	40	theme	lubricants	896:905	arg1	time					877:880	the service time	865:880	the service time of biomimetic lubricants	865:905	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	1	41	theme	due	234:236	arg1	surgery					226:232	serious wear after implantation surgery	194:232	serious wear after implantation surgery due to the poor lubrication of the body fluid	194:278	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	0	42	theme	ethylene	55:62	arg1	oxide/poly					44:53	graphene oxide/poly	35:53	graphene oxide/poly(ethylene glycol)	35:70	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	0	42	theme	ethylene	55:62	arg1	glycol					64:69	ethylene glycol	55:69	ethylene glycol	55:69	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	2	43	theme	lubrication	494:504	arg1	effect					506:511	slow-release lubrication effect	481:511	slow-release lubrication effect	481:511	However, the composite hydrogel with slow-release lubrication effect provides a new way for the lubrication of artificial joints.					
30813047	3	44	theme	oxide/Poly	598:607	arg1	composites					635:644	Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites	589:644	Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites	589:644	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	5	45	theme	hydrogen-bond	1182:1194	arg1	interaction					1196:1206	hydrogen-bond interaction	1182:1206	hydrogen-bond interaction	1182:1206	GO, PEG and small molecule GP in the slow-release solution through hydrogen-bond interaction might form a particular structure, which led to the good lubricating effect.					
30813047	5	46	theme	small	1127:1131	arg1	GP					1142:1143	small molecule GP	1127:1143	small molecule GP	1127:1143	GO, PEG and small molecule GP in the slow-release solution through hydrogen-bond interaction might form a particular structure, which led to the good lubricating effect.					
30813047	0	47	theme	artificial	136:145	arg1	joints					147:152	artificial joints	136:152	artificial joints	136:152	Slow-release lubrication effect of graphene oxide/poly(ethylene glycol) wrapped in chitosan/sodium glycerophosphate hydrogel applied on artificial joints.					
30813047	5	48	theme	particular	1221:1230	arg1	structure					1232:1240	a particular structure	1219:1240	a particular structure	1219:1240	GO, PEG and small molecule GP in the slow-release solution through hydrogen-bond interaction might form a particular structure, which led to the good lubricating effect.					
30813047	3	49	theme	biomimetic	885:894	arg1	lubricants					896:905	biomimetic lubricants	885:905	biomimetic lubricants	885:905	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	5	50	theme	slow-release	1152:1163	arg1	solution					1165:1172	the slow-release solution	1148:1172	the slow-release solution	1148:1172	GO, PEG and small molecule GP in the slow-release solution through hydrogen-bond interaction might form a particular structure, which led to the good lubricating effect.					
30813047	6	51	contain	had	1378:1380	arg1	GO					1348:1349	GO	1348:1349	GO	1348:1349	The experiments of cell and acute toxicity in vivo showed that GO and its composite hydrogel had good biocompatibility.					
30813047	6	51	contain	had	1378:1380	arg1	hydrogel					1369:1376	its composite hydrogel	1355:1376	its composite hydrogel	1355:1376	The experiments of cell and acute toxicity in vivo showed that GO and its composite hydrogel had good biocompatibility.					
30813047	6	51	contain	had	1378:1380	arg2	biocompatibility					1387:1402	good biocompatibility	1382:1402	good biocompatibility	1382:1402	The experiments of cell and acute toxicity in vivo showed that GO and its composite hydrogel had good biocompatibility.					
30813047	4	52	theme	increasing	1103:1112	arg1	pressure					1094:1101	the pressure increasing	1090:1112	the pressure increasing	1090:1112	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	4	53	theme	slow-release	1036:1047	arg1	solution					1049:1056	the slow-release solution	1032:1056	the slow-release solution	1032:1056	The friction experimental results showed that GO/PEG had better lubrication effect, and the average friction coefficient of the slow-release solution was below 0.03, especially with the pressure increasing.					
30813047	3	54	theme	CS/GP	766:770	arg1	hydrogel					773:780	the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel	728:780	the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel	728:780	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	2	55	theme	composite	457:465	arg1	hydrogel					467:474	the composite hydrogel	453:474	the composite hydrogel with slow-release lubrication effect	453:511	However, the composite hydrogel with slow-release lubrication effect provides a new way for the lubrication of artificial joints.					
30813047	1	56	theme	time	401:404	arg1	period					391:396	a period	389:396	a period of time	389:404	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	3	57	theme	lubricant	794:802	arg1	GO/PEG					787:792	the GO/PEG lubricant	783:802	the GO/PEG lubricant	783:802	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	1	58	theme	poor	245:248	arg1	lubrication					250:260	the poor lubrication	241:260	the poor lubrication of the body fluid	241:278	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	3	59	theme	glycerophosphate	748:763	arg1	hydrogel					773:780	the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel	728:780	the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel	728:780	In this study, Graphene oxide/Poly(ethylene glycol) (GO/PEG) composites were prepared to improve the artificial joint lubrication, and through wrapped in the Chitosan/Sodium glycerophosphate (CS/GP) hydrogel, the GO/PEG lubricant will be released under the squeezing action, thus to prolong the service time of biomimetic lubricants.					
30813047	1	60	theme	artificial	159:168	arg1	joints					170:175	The artificial joints	155:175	The artificial joints	155:175	The artificial joints would go through serious wear after implantation surgery due to the poor lubrication of the body fluid, and the biomimetic lubricants directly injected in vitro is easy to be absorbed by human tissues, and after a period of time, it will lose its lubrication effect.					
30813047	2	61	theme	slow-release	481:492	arg1	effect					506:511	slow-release lubrication effect	481:511	slow-release lubrication effect	481:511	However, the composite hydrogel with slow-release lubrication effect provides a new way for the lubrication of artificial joints.					
30738162	8	0	theme	2	1001:1001	arg1	%					1002:1002	%	1002:1002	%	1002:1002	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	9	1	theme	nBG	1079:1081	arg1	hydrogel					1083:1090	2%Ch-5%nBG hydrogel	1072:1090	2%Ch-5%nBG hydrogel	1072:1090	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	8	2	theme	%	1002:1002	arg1	hydrogel					1012:1019	2%Ch-5%nBG hydrogel	1001:1019	2%Ch-5%nBG hydrogel	1001:1019	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	5	3	theme	Sol-gel	679:685	arg1	method					687:692	Sol-gel method	679:692	Sol-gel method	679:692	Sol-gel method was followed to synthesize nBG particles and its particle size was found to be 14 ± 3 nm.					
30738162	4	4	theme	factor	469:474	arg1	XII					476:478	coagulation factor XII	457:478	coagulation factor XII	457:478	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	9	5	theme	%	1073:1073	arg1	hydrogel					1083:1090	2%Ch-5%nBG hydrogel	1072:1090	2%Ch-5%nBG hydrogel	1072:1090	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	8	6	theme	%	1007:1007	arg1	hydrogel					1012:1019	2%Ch-5%nBG hydrogel	1001:1019	2%Ch-5%nBG hydrogel	1001:1019	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	9	7	theme	great	1103:1107	arg1	potential					1109:1117	great potential	1103:1117	great potential to achieve effective bleeding control during critical situations	1103:1182	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	8	8	theme	Ch	1052:1053	arg1	hydrogel					1055:1062	2%Ch hydrogel	1050:1062	2%Ch hydrogel	1050:1062	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	8	9	theme	Ch-5	1003:1006	arg1	hydrogel					1012:1019	2%Ch-5%nBG hydrogel	1001:1019	2%Ch-5%nBG hydrogel	1001:1019	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	4	10	theme	coagulation	457:467	arg1	XII					476:478	coagulation factor XII	457:478	coagulation factor XII	457:478	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	4	11	theme	protonated	586:595	arg1	hydrogel					576:583	Ch hydrogel	573:583	Ch hydrogel (protonated NH2 group)	573:606	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	4	11	theme	protonated	586:595	arg1	group					601:605	protonated NH2 group	586:605	protonated NH2 group	586:605	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	2	12	theme	blood	290:294	arg1	cells					296:300	blood cells	290:300	blood cells	290:300	Chitosan (Ch) as hemostatic agent has been widely used and when applied at the site of injury it acts by aggregating blood cells and forming a plug.					
30738162	6	13	theme	Ch-5	786:789	arg1	hydrogel					795:802	2%Ch-5%nBG hydrogel	784:802	2%Ch-5%nBG hydrogel	784:802	2%Ch-5%nBG hydrogel was then prepared and studied using SEM and FTIR.					
30738162	2	14	theme	injury	260:265	arg1	injury					260:265	injury	260:265	injury	260:265	Chitosan (Ch) as hemostatic agent has been widely used and when applied at the site of injury it acts by aggregating blood cells and forming a plug.					
30738162	2	14	theme	injury	260:265	arg1	site					252:255	the site	248:255	the site of injury	248:265	Chitosan (Ch) as hemostatic agent has been widely used and when applied at the site of injury it acts by aggregating blood cells and forming a plug.					
30738162	4	15	theme	clot	663:666	arg1	formation					668:676	rapid blood clot formation	651:676	rapid blood clot formation	651:676	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	4	16	theme	phosphate	523:531	arg1	ions					563:566	calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions	482:566	calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions	482:566	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	4	16	theme	phosphate	523:531	arg1	nanobioglass					416:427	nanobioglass	416:427	nanobioglass (nBG) with silica (activate coagulation factor XII)	416:479	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	8	17	theme	nBG	1008:1010	arg1	hydrogel					1012:1019	2%Ch-5%nBG hydrogel	1001:1019	2%Ch-5%nBG hydrogel	1001:1019	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	4	18	theme	same	625:628	arg1	time					630:633	the same time	621:633	the same time	621:633	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	8	19	theme	injury	987:992	arg1	model					994:998	in vivo major organ injury model	967:998	in vivo major organ injury model	967:998	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	3	20	theme	hydrogel	389:396	arg1	property					374:381	the blood clotting property	355:381	the blood clotting property of Ch hydrogel	355:396	Our prime interest is to improve the blood clotting property of Ch hydrogel.					
30738162	8	21	theme	2	1050:1050	arg1	%					1051:1051	%	1051:1051	%	1051:1051	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	6	22	theme	2	784:784	arg1	%					785:785	%	785:785	%	785:785	2%Ch-5%nBG hydrogel was then prepared and studied using SEM and FTIR.					
30738162	9	23	contain	have	1098:1101	arg1	hydrogel					1083:1090	2%Ch-5%nBG hydrogel	1072:1090	2%Ch-5%nBG hydrogel	1072:1090	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	9	23	contain	have	1098:1101	arg2	potential					1109:1117	great potential	1103:1117	great potential to achieve effective bleeding control during critical situations	1103:1182	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	1	24	theme	Effective	96:104	arg1	control					115:121	Effective bleeding control	96:121	Effective bleeding control	96:121	Effective bleeding control is a major concern in trauma and major surgeries.					
30738162	1	24	theme	Effective	96:104	arg1	concern					134:140	a major concern	126:140	a major concern in trauma and major surgeries	126:170	Effective bleeding control is a major concern in trauma and major surgeries.					
30738162	4	25	with	nanobioglass	416:427	arg1	silica					440:445	silica	440:445	silica (activate coagulation factor XII)	440:479	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	1	26	theme	bleeding	106:113	arg1	control					115:121	Effective bleeding control	96:121	Effective bleeding control	96:121	Effective bleeding control is a major concern in trauma and major surgeries.					
30738162	1	26	theme	bleeding	106:113	arg1	concern					134:140	a major concern	126:140	a major concern in trauma and major surgeries	126:170	Effective bleeding control is a major concern in trauma and major surgeries.					
30738162	6	27	theme	%	785:785	arg1	hydrogel					795:802	2%Ch-5%nBG hydrogel	784:802	2%Ch-5%nBG hydrogel	784:802	2%Ch-5%nBG hydrogel was then prepared and studied using SEM and FTIR.					
30738162	0	28	theme	Injectable	0:9	arg1	chitosan-nano					11:23	Injectable chitosan-nano	0:23	Injectable chitosan-nano	0:23	Injectable chitosan-nano bioglass composite hemostatic hydrogel for effective bleeding control.					
30738162	4	29	dep	silica	440:445	arg1	activate					448:455	activate	448:455	activate coagulation factor XII	448:478	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	8	30	theme	in	933:934	arg1	study					957:961	in vitro blood clotting study	933:961	in vitro blood clotting study	933:961	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	9	31	theme	%	1078:1078	arg1	hydrogel					1083:1090	2%Ch-5%nBG hydrogel	1072:1090	2%Ch-5%nBG hydrogel	1072:1090	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	3	32	theme	Ch	386:387	arg1	hydrogel					389:396	Ch hydrogel	386:396	Ch hydrogel	386:396	Our prime interest is to improve the blood clotting property of Ch hydrogel.					
30738162	0	33	theme	composite	34:42	arg1	hydrogel					55:62	composite hemostatic hydrogel	34:62	composite hemostatic hydrogel for effective bleeding control	34:93	Injectable chitosan-nano bioglass composite hemostatic hydrogel for effective bleeding control.					
30738162	5	34	theme	nBG	721:723	arg1	particles					725:733	nBG particles	721:733	nBG particles	721:733	Sol-gel method was followed to synthesize nBG particles and its particle size was found to be 14 ± 3 nm.					
30738162	8	35	theme	clotting	948:955	arg1	study					957:961	in vitro blood clotting study	933:961	in vitro blood clotting study	933:961	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	1	36	from	concern	134:140	arg1	trauma					145:150	trauma	145:150	trauma	145:150	Effective bleeding control is a major concern in trauma and major surgeries.					
30738162	1	36	from	concern	134:140	arg1	surgeries					162:170	major surgeries	156:170	major surgeries	156:170	Effective bleeding control is a major concern in trauma and major surgeries.					
30738162	4	37	theme	blood	657:661	arg1	formation					668:676	rapid blood clot formation	651:676	rapid blood clot formation	651:676	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	8	38	theme	rapid	1028:1032	arg1	clot					1040:1043	rapid blood clot	1028:1043	rapid blood clot	1028:1043	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	9	39	theme	bleeding	1140:1147	arg1	control					1149:1155	effective bleeding control	1130:1155	effective bleeding control	1130:1155	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	8	40	theme	blood	942:946	arg1	study					957:961	in vitro blood clotting study	933:961	in vitro blood clotting study	933:961	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	4	41	theme	rapid	651:655	arg1	formation					668:676	rapid blood clot formation	651:676	rapid blood clot formation	651:676	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	2	42	used	used	223:226	arg2	Chitosan					173:180	Chitosan	173:180	Chitosan (Ch) as hemostatic agent	173:205	Chitosan (Ch) as hemostatic agent has been widely used and when applied at the site of injury it acts by aggregating blood cells and forming a plug.					
30738162	2	42	used	used	223:226	arg2	Ch					183:184	Ch	183:184	Ch	183:184	Chitosan (Ch) as hemostatic agent has been widely used and when applied at the site of injury it acts by aggregating blood cells and forming a plug.					
30738162	5	43	theme	particle	743:750	arg1	size					752:755	its particle size	739:755	its particle size	739:755	Sol-gel method was followed to synthesize nBG particles and its particle size was found to be 14 ± 3 nm.					
30738162	3	44	theme	clotting	365:372	arg1	property					374:381	the blood clotting property	355:381	the blood clotting property of Ch hydrogel	355:396	Our prime interest is to improve the blood clotting property of Ch hydrogel.					
30738162	4	45	theme	intrinsic	500:508	arg1	pathway					510:516	intrinsic pathway	500:516	intrinsic pathway	500:516	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	8	46	dep	in	933:934	arg1	vitro					936:940	vitro	936:940	vitro	936:940	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	4	47	dep	phosphate	523:531	arg1	initiates					534:542	initiates	534:542	initiates extrinsic pathway	534:560	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	4	48	theme	NH2	597:599	arg1	hydrogel					576:583	Ch hydrogel	573:583	Ch hydrogel (protonated NH2 group)	573:606	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	4	48	theme	NH2	597:599	arg1	group					601:605	protonated NH2 group	586:605	protonated NH2 group	586:605	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	9	49	theme	effective	1130:1138	arg1	control					1149:1155	effective bleeding control	1130:1155	effective bleeding control	1130:1155	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	0	50	theme	hemostatic	44:53	arg1	hydrogel					55:62	composite hemostatic hydrogel	34:62	composite hemostatic hydrogel for effective bleeding control	34:93	Injectable chitosan-nano bioglass composite hemostatic hydrogel for effective bleeding control.					
30738162	8	51	theme	blood	1034:1038	arg1	clot					1040:1043	rapid blood clot	1028:1043	rapid blood clot	1028:1043	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	4	52	theme	Ch	573:574	arg1	hydrogel					576:583	Ch hydrogel	573:583	Ch hydrogel (protonated NH2 group)	573:606	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	4	52	theme	Ch	573:574	arg1	group					601:605	protonated NH2 group	586:605	protonated NH2 group	586:605	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	0	53	theme	effective	68:76	arg1	control					87:93	effective bleeding control	68:93	effective bleeding control	68:93	Injectable chitosan-nano bioglass composite hemostatic hydrogel for effective bleeding control.					
30738162	9	54	theme	critical	1164:1171	arg1	situations					1173:1182	critical situations	1164:1182	critical situations	1164:1182	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	4	55	theme	nanobioglass	416:427	arg1	Incorporation					399:411	Incorporation	399:411	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group)	399:606	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	1	56	theme	major	128:132	arg1	control					115:121	Effective bleeding control	96:121	Effective bleeding control	96:121	Effective bleeding control is a major concern in trauma and major surgeries.					
30738162	1	56	theme	major	128:132	arg1	concern					134:140	a major concern	126:140	a major concern in trauma and major surgeries	126:170	Effective bleeding control is a major concern in trauma and major surgeries.					
30738162	3	57	theme	blood	359:363	arg1	property					374:381	the blood clotting property	355:381	the blood clotting property of Ch hydrogel	355:396	Our prime interest is to improve the blood clotting property of Ch hydrogel.					
30738162	4	58	theme	calcium	482:488	arg1	ions					563:566	calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions	482:566	calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions	482:566	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	4	58	theme	calcium	482:488	arg1	nanobioglass					416:427	nanobioglass	416:427	nanobioglass (nBG) with silica (activate coagulation factor XII)	416:479	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	8	59	theme	in	967:968	arg1	model					994:998	in vivo major organ injury model	967:998	in vivo major organ injury model	967:998	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	6	60	theme	nBG	791:793	arg1	hydrogel					795:802	2%Ch-5%nBG hydrogel	784:802	2%Ch-5%nBG hydrogel	784:802	2%Ch-5%nBG hydrogel was then prepared and studied using SEM and FTIR.					
30738162	8	61	theme	organ	981:985	arg1	model					994:998	in vivo major organ injury model	967:998	in vivo major organ injury model	967:998	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	9	62	theme	2	1072:1072	arg1	%					1073:1073	%	1073:1073	%	1073:1073	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	4	63	theme	extrinsic	544:552	arg1	pathway					554:560	extrinsic pathway	544:560	extrinsic pathway	544:560	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	2	64	theme	hemostatic	190:199	arg1	agent					201:205	hemostatic agent	190:205	hemostatic agent	190:205	Chitosan (Ch) as hemostatic agent has been widely used and when applied at the site of injury it acts by aggregating blood cells and forming a plug.					
30738162	7	65	theme	prepared	858:865	arg1	injecable					880:888	injecable	880:888	injecable	880:888	The prepared hydrogel was injecable and was also cytocompatible with HUVEC.					
30738162	7	65	theme	prepared	858:865	arg1	hydrogel					867:874	The prepared hydrogel	854:874	The prepared hydrogel	854:874	The prepared hydrogel was injecable and was also cytocompatible with HUVEC.					
30738162	7	65	theme	prepared	858:865	arg1	cytocompatible					903:916	cytocompatible	903:916	cytocompatible	903:916	The prepared hydrogel was injecable and was also cytocompatible with HUVEC.					
30738162	8	66	theme	%	1051:1051	arg1	hydrogel					1055:1062	2%Ch hydrogel	1050:1062	2%Ch hydrogel	1050:1062	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	9	67	theme	Ch-5	1074:1077	arg1	hydrogel					1083:1090	2%Ch-5%nBG hydrogel	1072:1090	2%Ch-5%nBG hydrogel	1072:1090	Hence, 2%Ch-5%nBG hydrogel might have great potential to achieve effective bleeding control during critical situations.					
30738162	7	68	with	cytocompatible	903:916	arg1	HUVEC					923:927	HUVEC	923:927	HUVEC	923:927	The prepared hydrogel was injecable and was also cytocompatible with HUVEC.					
30738162	6	69	theme	%	790:790	arg1	hydrogel					795:802	2%Ch-5%nBG hydrogel	784:802	2%Ch-5%nBG hydrogel	784:802	2%Ch-5%nBG hydrogel was then prepared and studied using SEM and FTIR.					
30738162	0	70	theme	bleeding	78:85	arg1	control					87:93	effective bleeding control	68:93	effective bleeding control	68:93	Injectable chitosan-nano bioglass composite hemostatic hydrogel for effective bleeding control.					
30738162	4	71	dep	calcium	482:488	arg1	activate					491:498	activate	491:498	activate intrinsic pathway	491:516	Incorporation of nanobioglass (nBG) with silica (activate coagulation factor XII), calcium (activate intrinsic pathway) and phosphate (initiates extrinsic pathway) ions into Ch hydrogel (protonated NH2 group) would act at the same time and bring about rapid blood clot formation.					
30738162	8	72	dep	in	967:968	arg1	vivo					970:973	vivo	970:973	vivo	970:973	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	8	72	dep	in	967:968	arg1	major					975:979	major	975:979	major	975:979	In in vitro blood clotting study and in vivo major organ injury model, 2%Ch-5%nBG hydrogel formed rapid blood clot than 2%Ch hydrogel.					
30738162	1	73	theme	major	156:160	arg1	surgeries					162:170	major surgeries	156:170	major surgeries	156:170	Effective bleeding control is a major concern in trauma and major surgeries.					
30738162	3	74	theme	prime	326:330	arg1	interest					332:339	Our prime interest	322:339	Our prime interest	322:339	Our prime interest is to improve the blood clotting property of Ch hydrogel.					
29659957	3	0	from	focus	618:622	arg1	barriers					655:662	colonic tight junction (TJ) barriers	627:662	colonic tight junction (TJ) barriers in mice	627:670	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	7	1	theme	inflammatory	1185:1196	arg1	cytokines					1198:1206	renal inflammatory cytokines	1179:1206	renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6	1179:1275	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	3	2	theme	GG	511:512	arg1	effects					411:417	the effects	407:417	the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development	407:597	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	4	3	dep	Methods	673:679	arg1	fed					709:711	fed	709:711	were fed an AIN-93G diet that contained 0.25% adenine for 2 wk	704:765	Methods Male 7-wk-old ICR mice were fed an AIN-93G diet that contained 0.25% adenine for 2 wk to induce CKD.					
29659957	6	4	theme	AIN-93G	966:972	arg1	diet					974:977	an AIN-93G diet	963:977	an AIN-93G diet without adenine	963:993	Control (CON) mice were fed an AIN-93G diet without adenine throughout the 5-wk experiment.					
29659957	1	5	theme	health	161:166	arg1	problem					168:174	a worldwide health problem	149:174	a worldwide health problem	149:174	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	1	5	theme	health	161:166	arg1	disease					132:138	Background Chronic kidney disease	106:138	Background Chronic kidney disease (CKD)	106:144	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	7	6	theme	necrosis	1214:1221	arg1	Tnfa					1233:1236	Tnfa	1233:1236	Tnfa	1233:1236	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	6	theme	necrosis	1214:1221	arg1	α					1230:1230	tumor necrosis factor α	1208:1230	tumor necrosis factor α (Tnfa)	1208:1237	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	7	theme	claudin	1161:1167	arg1	isoforms					1169:1176	claudin isoforms	1161:1176	claudin isoforms	1161:1176	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	2	8	theme	dysregulated	290:301	arg1	microflora					303:312	a dysregulated microflora	288:312	a dysregulated microflora	288:312	Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development.					
29659957	3	9	from	barriers	655:662	arg1	mice					667:670	mice	667:670	mice	667:670	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	10	10	theme	microflora	2153:2162	arg1	composition					2164:2174	microflora composition	2153:2174	microflora composition	2153:2174	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	10	11	theme	Conclusion	1976:1985	arg1	feeding					2000:2006	Conclusion Supplemental feeding	1976:2006	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG,	1976:2049	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	9	12	theme	Lactobacillus	1908:1920	arg1	4.0-fold					1931:1938	4.0-fold	1931:1938	4.0-fold	1931:1938	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	12	theme	Lactobacillus	1908:1920	arg1	concentrations					1941:1954	higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations	1867:1954	concentrations	1941:1954	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	13	dep	3.7-fold	1741:1748	arg1	to					1738:1739	to	1738:1739	to	1738:1739	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	8	14	from	kidney	1541:1546	arg1	2.2-					1430:1433	a 2.2-	1428:1433	a 2.2- to 4.4-fold higher plasma urea concentration	1428:1478	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	14	from	kidney	1541:1546	arg1	expression					1492:1501	greater expression	1484:1501	greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold)	1484:1632	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	9	15	dep	JAMA	1805:1808	arg1	4.4-					1811:1814	4.4-	1811:1814	4.4-	1811:1814	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	8	16	from	expression	1492:1501	arg1	kidney					1541:1546	the kidney	1537:1546	the kidney	1537:1546	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	2	17	theme	CKD	206:208	arg1	unclear					219:225	unclear	219:225	unclear	219:225	Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development.					
29659957	2	17	theme	CKD	206:208	arg1	pathogenesis					190:201	the pathogenesis	186:201	the pathogenesis of CKD	186:208	Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development.					
29659957	1	18	theme	kidney	125:130	arg1	disease					132:138	Background Chronic kidney disease	106:138	Background Chronic kidney disease (CKD)	106:144	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	1	18	theme	kidney	125:130	arg1	problem					168:174	a worldwide health problem	149:174	a worldwide health problem	149:174	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	1	18	theme	kidney	125:130	arg1	CKD					141:143	CKD	141:143	CKD	141:143	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	6	19	theme	5-wk	1010:1013	arg1	experiment					1015:1024	the 5-wk experiment	1006:1024	the 5-wk experiment	1006:1024	Control (CON) mice were fed an AIN-93G diet without adenine throughout the 5-wk experiment.					
29659957	7	20	theme	adhesion	1129:1136	arg1	proteins					1069:1076	the colonic TJ proteins	1054:1076	the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms	1054:1176	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	20	theme	adhesion	1129:1136	arg1	JAMA					1150:1153	JAMA	1150:1153	JAMA	1150:1153	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	20	theme	adhesion	1129:1136	arg1	A					1147:1147	junctional adhesion molecule A	1118:1147	junctional adhesion molecule A (JAMA)	1118:1154	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	10	21	theme	fermentable	2013:2023	arg1	DFs					2025:2027	fermentable DFs	2013:2027	fermentable DFs	2013:2027	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	9	22	theme	higher	1867:1872	arg1	SCFA					1880:1883	higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations	1867:1954	SCFA	1880:1883	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	22	theme	higher	1867:1872	arg1	3.5-fold					1894:1901	3.5-fold	1894:1901	3.5-fold	1894:1901	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	8	23	theme	higher	1447:1452	arg1	concentration					1466:1478	4.4-fold higher plasma urea concentration	1438:1478	4.4-fold higher plasma urea concentration	1438:1478	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	7	24	dep	cytokines	1198:1206	arg1	Il6					1273:1275	Il6	1273:1275	Il6	1273:1275	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	24	dep	cytokines	1198:1206	arg1	Tnfa					1233:1236	Tnfa	1233:1236	Tnfa	1233:1236	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	24	dep	cytokines	1198:1206	arg1	α					1230:1230	tumor necrosis factor α	1208:1230	tumor necrosis factor α (Tnfa)	1208:1237	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	24	dep	cytokines	1198:1206	arg1	Il1b					1262:1265	Il1b	1262:1265	Il1b	1262:1265	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	24	dep	cytokines	1198:1206	arg1	1β					1258:1259	interleukin (Il ) 1β	1240:1259	interleukin (Il ) 1β (Il1b)	1240:1266	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	25	theme	fatty	1300:1304	arg1	SCFAs					1313:1317	SCFAs	1313:1317	SCFAs	1313:1317	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	25	theme	fatty	1300:1304	arg1	acids					1306:1310	cecal short-chain fatty acids	1282:1310	cecal short-chain fatty acids (SCFAs)	1282:1318	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	8	26	from	genes	1528:1532	arg1	kidney					1541:1546	the kidney	1537:1546	the kidney	1537:1546	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	7	27	theme	urea	1034:1037	arg1	concentration					1039:1051	Plasma urea concentration	1027:1051	Plasma urea concentration	1027:1051	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	3	28	theme	viscosity	553:561	arg1	characteristics					563:577	distinct viscosity characteristics	544:577	distinct viscosity characteristics	544:577	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	8	29	theme	urea	1461:1464	arg1	concentration					1466:1478	4.4-fold higher plasma urea concentration	1438:1478	4.4-fold higher plasma urea concentration	1438:1478	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	7	30	theme	cecal	1282:1286	arg1	SCFAs					1313:1317	SCFAs	1313:1317	SCFAs	1313:1317	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	30	theme	cecal	1282:1286	arg1	acids					1306:1310	cecal short-chain fatty acids	1282:1310	cecal short-chain fatty acids (SCFAs)	1282:1318	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	8	31	dep	Tnfa	1559:1562	arg1	48-fold					1573:1579	48-fold	1573:1579	48-fold	1573:1579	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	9	32	dep	4.3-fold	1765:1772	arg1	to					1762:1763	to	1762:1763	to	1762:1763	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	4	33	contain	contained	734:742	arg2	adenine					750:756	0.25% adenine	744:756	0.25% adenine	744:756	Methods Male 7-wk-old ICR mice were fed an AIN-93G diet that contained 0.25% adenine for 2 wk to induce CKD.					
29659957	4	33	contain	contained	734:742	arg1	diet					724:727	an AIN-93G diet	713:727	an AIN-93G diet that contained 0.25% adenine	713:756	Methods Male 7-wk-old ICR mice were fed an AIN-93G diet that contained 0.25% adenine for 2 wk to induce CKD.					
29659957	4	34	theme	7-wk-old	686:693	arg1	mice					699:702	Male 7-wk-old ICR mice	681:702	Male 7-wk-old ICR mice	681:702	Methods Male 7-wk-old ICR mice were fed an AIN-93G diet that contained 0.25% adenine for 2 wk to induce CKD.					
29659957	0	35	theme	Intestinal	86:95	arg1	Barrier					97:103	the Intestinal Barrier	82:103	the Intestinal Barrier	82:103	Dietary Fermentable Fibers Attenuate Chronic Kidney Disease in Mice by Protecting the Intestinal Barrier.					
29659957	9	36	theme	cecal	1874:1878	arg1	SCFA					1880:1883	higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations	1867:1954	SCFA	1880:1883	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	36	theme	cecal	1874:1878	arg1	3.5-fold					1894:1901	3.5-fold	1894:1901	3.5-fold	1894:1901	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	7	37	dep	proteins	1069:1076	arg1	ZO2					1103:1105	ZO2	1103:1105	ZO2	1103:1105	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	37	dep	proteins	1069:1076	arg1	isoforms					1169:1176	claudin isoforms	1161:1176	claudin isoforms	1161:1176	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	37	dep	proteins	1069:1076	arg1	A					1147:1147	junctional adhesion molecule A	1118:1147	junctional adhesion molecule A (JAMA)	1118:1154	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	37	dep	proteins	1069:1076	arg1	occludin					1108:1115	occludin	1108:1115	occludin	1108:1115	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	37	dep	proteins	1069:1076	arg1	JAMA					1150:1153	JAMA	1150:1153	JAMA	1150:1153	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	37	dep	proteins	1069:1076	arg1	ZO					1096:1097	ZO	1096:1097	ZO	1096:1097	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	37	dep	proteins	1069:1076	arg1	proteins					1069:1076	the colonic TJ proteins	1054:1076	the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms	1054:1176	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	37	dep	proteins	1069:1076	arg1	occludens					1085:1093	zonula occludens (ZO) 1	1078:1100	zonula occludens (ZO) 1	1078:1100	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	3	38	dep	GG	511:512	arg1	substances					528:537	substances	528:537	substances with distinct viscosity characteristics	528:577	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	0	39	from	Disease	52:58	arg1	Mice					63:66	Mice	63:66	Mice	63:66	Dietary Fermentable Fibers Attenuate Chronic Kidney Disease in Mice by Protecting the Intestinal Barrier.					
29659957	3	40	theme	fermentable	426:436	arg1	DFs					454:456	DFs	454:456	DFs	454:456	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	3	40	theme	fermentable	426:436	arg1	fibers					446:451	the fermentable dietary fibers	422:451	the fermentable dietary fibers (DFs)	422:457	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	10	41	with	feeding	2000:2006	arg1	DFs					2025:2027	fermentable DFs	2013:2027	fermentable DFs	2013:2027	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	10	41	with	feeding	2000:2006	arg1	GG					2038:2039	GG	2038:2039	GG	2038:2039	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	8	42	theme	inflammatory	1506:1517	arg1	Tnfa					1559:1562	Tnfa	1559:1562	Tnfa (4.4- to 48-fold)	1559:1580	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	42	theme	inflammatory	1506:1517	arg1	Il1b					1583:1586	Il1b	1583:1586	Il1b (4.6- to 56-fold)	1583:1604	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	42	theme	inflammatory	1506:1517	arg1	Il6					1611:1613	Il6	1611:1613	Il6 (8.8- to 115-fold)	1611:1632	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	42	theme	inflammatory	1506:1517	arg1	genes					1528:1532	inflammatory cytokine genes	1506:1532	inflammatory cytokine genes	1506:1532	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	9	43	theme	2.9-	1733:1736	arg1	ZO1					1728:1730	ZO1	1728:1730	ZO1 (2.9- to 3.7-fold)	1728:1749	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	43	theme	2.9-	1733:1736	arg1	3.7-fold					1741:1748	2.9- to 3.7-fold	1733:1748	2.9- to 3.7-fold	1733:1748	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	0	44	theme	Dietary	0:6	arg1	Fibers					20:25	Dietary Fermentable Fibers	0:25	Dietary Fermentable Fibers	0:25	Dietary Fermentable Fibers Attenuate Chronic Kidney Disease in Mice by Protecting the Intestinal Barrier.					
29659957	8	45	theme	genes	1528:1532	arg1	2.2-					1430:1433	a 2.2-	1428:1433	a 2.2- to 4.4-fold higher plasma urea concentration	1428:1478	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	45	theme	genes	1528:1532	arg1	expression					1492:1501	greater expression	1484:1501	greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold)	1484:1632	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	2	46	theme	recent	228:233	arg1	studies					235:241	recent studies	228:241	recent studies	228:241	Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development.					
29659957	1	47	theme	Background	106:115	arg1	disease					132:138	Background Chronic kidney disease	106:138	Background Chronic kidney disease (CKD)	106:144	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	1	47	theme	Background	106:115	arg1	problem					168:174	a worldwide health problem	149:174	a worldwide health problem	149:174	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	1	47	theme	Background	106:115	arg1	CKD					141:143	CKD	141:143	CKD	141:143	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	9	48	theme	CKD+GG	1658:1663	arg1	groups					1665:1670	The CON, CKD+PHGG, and CKD+GG groups	1635:1670	groups	1665:1670	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	10	49	theme	CKD	2106:2108	arg1	management					2092:2101	management	2092:2101	management	2092:2101	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	10	49	theme	CKD	2106:2108	arg1	prevention					2078:2087	prevention	2078:2087	prevention	2078:2087	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	3	50	theme	fibers	446:451	arg1	effects					411:417	the effects	407:417	the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development	407:597	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	0	51	theme	Chronic	37:43	arg1	Disease					52:58	Chronic Kidney Disease	37:58	Chronic Kidney Disease in Mice	37:66	Dietary Fermentable Fibers Attenuate Chronic Kidney Disease in Mice by Protecting the Intestinal Barrier.					
29659957	9	52	dep	4.0-fold	1931:1938	arg1	to					1928:1929	to	1928:1929	to	1928:1929	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	8	53	dep	48-fold	1573:1579	arg1	to					1570:1571	to	1570:1571	to	1570:1571	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	3	54	theme	CKD	583:585	arg1	development					587:597	CKD development	583:597	CKD development	583:597	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	5	55	theme	unsupplemented	843:856	arg1	CKD					864:866	CKD	864:866	CKD	864:866	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	5	55	theme	unsupplemented	843:856	arg1	diet					858:861	the unsupplemented diet	839:861	the unsupplemented diet (CKD)	839:867	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	3	56	theme	guar	471:474	arg1	GG					481:482	GG	481:482	GG	481:482	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	3	56	theme	guar	471:474	arg1	gum					476:478	unmodified guar gum	460:478	unmodified guar gum (GG)	460:483	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	8	57	contain	had	1424:1426	arg2	2.2-					1430:1433	a 2.2-	1428:1433	a 2.2- to 4.4-fold higher plasma urea concentration	1428:1478	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	57	contain	had	1424:1426	arg2	expression					1492:1501	greater expression	1484:1501	greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold)	1484:1632	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	57	contain	had	1424:1426	arg1	group					1418:1422	the CKD group	1410:1422	the CKD group	1410:1422	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	7	58	theme	Il 	1253:1255	arg1	Il1b					1262:1265	Il1b	1262:1265	Il1b	1262:1265	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	58	theme	Il 	1253:1255	arg1	1β					1258:1259	interleukin (Il ) 1β	1240:1259	interleukin (Il ) 1β (Il1b)	1240:1266	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	5	59	theme	%	892:892	arg1	CKD+PHGG					900:907	CKD+PHGG	900:907	CKD+PHGG	900:907	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	5	59	theme	%	892:892	arg1	PHGG					894:897	10% PHGG	890:897	10% PHGG (CKD+PHGG)	890:908	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	9	60	contain	had	1672:1674	arg2	expression					1684:1693	greater expression	1676:1693	greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold)	1676:1861	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	60	contain	had	1672:1674	arg2	2.1-					1845:1848	2.1- to 2.6-fold	1845:1860	2.1- to 2.6-fold	1845:1860	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	60	contain	had	1672:1674	arg1	groups					1665:1670	The CON, CKD+PHGG, and CKD+GG groups	1635:1670	groups	1665:1670	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	60	contain	had	1672:1674	arg2	SCFA					1880:1883	higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations	1867:1954	SCFA	1880:1883	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	60	contain	had	1672:1674	arg1	CKD+PHGG					1644:1651	The CON, CKD+PHGG, and CKD+GG groups	1635:1670	CKD+PHGG	1644:1651	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	60	contain	had	1672:1674	arg2	3.5-fold					1894:1901	3.5-fold	1894:1901	3.5-fold	1894:1901	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	10	61	theme	barrier	2131:2137	arg1	integrity					2139:2147	colonic barrier integrity	2123:2147	colonic barrier integrity	2123:2147	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	10	62	dep	prevention	2078:2087	arg1	the					2074:2076	the	2074:2076	the	2074:2076	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	7	63	theme	interleukin	1240:1250	arg1	Il1b					1262:1265	Il1b	1262:1265	Il1b	1262:1265	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	63	theme	interleukin	1240:1250	arg1	1β					1258:1259	interleukin (Il ) 1β	1240:1259	interleukin (Il ) 1β (Il1b)	1240:1266	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	5	64	contain	containing	879:888	arg2	CKD+GG					917:922	CKD+GG	917:922	CKD+GG	917:922	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	5	64	contain	containing	879:888	arg2	PHGG					894:897	10% PHGG	890:897	10% PHGG (CKD+PHGG)	890:908	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	5	64	contain	containing	879:888	arg2	GG					913:914	GG	913:914	GG (CKD+GG)	913:923	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	5	64	contain	containing	879:888	arg1	diet					874:877	a diet	872:877	a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk	872:932	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	5	64	contain	containing	879:888	arg2	CKD+PHGG					900:907	CKD+PHGG	900:907	CKD+PHGG	900:907	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	9	65	theme	colonic	1698:1704	arg1	JAMA					1805:1808	JAMA	1805:1808	JAMA (4.4- to 5.4-fold)	1805:1827	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	65	theme	colonic	1698:1704	arg1	claudin					1834:1840	claudin 7	1834:1842	claudin 7	1834:1842	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	65	theme	colonic	1698:1704	arg1	proteins					1709:1716	colonic TJ proteins	1698:1716	colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7	1698:1842	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	65	theme	colonic	1698:1704	arg1	ZO1					1728:1730	ZO1	1728:1730	ZO1 (2.9- to 3.7-fold)	1728:1749	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	65	theme	colonic	1698:1704	arg1	ZO2					1752:1754	ZO2	1752:1754	ZO2 (3.4- to 4.3-fold)	1752:1773	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	65	theme	colonic	1698:1704	arg1	occludin					1776:1783	occludin	1776:1783	occludin (3.0- to 3.3-fold)	1776:1802	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	4	66	theme	AIN-93G	716:722	arg1	diet					724:727	an AIN-93G diet	713:727	an AIN-93G diet that contained 0.25% adenine	713:756	Methods Male 7-wk-old ICR mice were fed an AIN-93G diet that contained 0.25% adenine for 2 wk to induce CKD.					
29659957	10	67	dep	such	2030:2033	arg1	as					2035:2036	as	2035:2036	as	2035:2036	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	9	68	dep	ZO2	1752:1754	arg1	4.3-fold					1765:1772	4.3-fold	1765:1772	4.3-fold	1765:1772	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	7	69	theme	colonic	1058:1064	arg1	proteins					1069:1076	the colonic TJ proteins	1054:1076	the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms	1054:1176	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	69	theme	colonic	1058:1064	arg1	occludin					1108:1115	occludin	1108:1115	occludin	1108:1115	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	69	theme	colonic	1058:1064	arg1	occludens					1085:1093	zonula occludens (ZO) 1	1078:1100	zonula occludens (ZO) 1	1078:1100	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	69	theme	colonic	1058:1064	arg1	A					1147:1147	junctional adhesion molecule A	1118:1147	junctional adhesion molecule A (JAMA)	1118:1154	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	69	theme	colonic	1058:1064	arg1	ZO2					1103:1105	ZO2	1103:1105	ZO2	1103:1105	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	3	70	theme	junction	641:648	arg1	barriers					655:662	colonic tight junction (TJ) barriers	627:662	colonic tight junction (TJ) barriers in mice	627:670	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	9	71	theme	proteins	1709:1716	arg1	3.5-fold					1894:1901	3.5-fold	1894:1901	3.5-fold	1894:1901	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	71	theme	proteins	1709:1716	arg1	4.0-fold					1931:1938	4.0-fold	1931:1938	4.0-fold	1931:1938	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	71	theme	proteins	1709:1716	arg1	2.1-					1845:1848	2.1- to 2.6-fold	1845:1860	2.1- to 2.6-fold	1845:1860	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	71	theme	proteins	1709:1716	arg1	concentrations					1941:1954	higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations	1867:1954	concentrations	1941:1954	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	71	theme	proteins	1709:1716	arg1	SCFA					1880:1883	higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations	1867:1954	SCFA	1880:1883	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	71	theme	proteins	1709:1716	arg1	expression					1684:1693	greater expression	1676:1693	greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold)	1676:1861	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	3	72	theme	colonic	627:633	arg1	TJ					651:652	TJ	651:652	TJ	651:652	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	3	72	theme	colonic	627:633	arg1	junction					641:648	colonic tight junction	627:648	colonic tight junction (TJ) barriers in mice	627:670	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	4	73	theme	0.25	744:747	arg1	%					748:748	%	748:748	%	748:748	Methods Male 7-wk-old ICR mice were fed an AIN-93G diet that contained 0.25% adenine for 2 wk to induce CKD.					
29659957	3	74	theme	hydrolyzed	500:509	arg1	PHGG					515:518	PHGG	515:518	PHGG	515:518	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	3	74	theme	hydrolyzed	500:509	arg1	GG					511:512	partially hydrolyzed GG	490:512	partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics)	490:578	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	7	75	theme	factor	1223:1228	arg1	Tnfa					1233:1236	Tnfa	1233:1236	Tnfa	1233:1236	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	75	theme	factor	1223:1228	arg1	α					1230:1230	tumor necrosis factor α	1208:1230	tumor necrosis factor α (Tnfa)	1208:1237	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	9	76	dep	2.1-	1845:1848	arg1	to					1850:1851	to	1850:1851	to	1850:1851	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	2	77	theme	intestinal	330:339	arg1	barrier					341:347	an impaired intestinal barrier	318:347	an impaired intestinal barrier	318:347	Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development.					
29659957	2	78	theme	systemic	256:263	arg1	inflammation					265:276	systemic inflammation	256:276	systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier	256:347	Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development.					
29659957	1	79	theme	worldwide	151:159	arg1	problem					168:174	a worldwide health problem	149:174	a worldwide health problem	149:174	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	1	79	theme	worldwide	151:159	arg1	disease					132:138	Background Chronic kidney disease	106:138	Background Chronic kidney disease (CKD)	106:144	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	7	80	theme	tumor	1208:1212	arg1	Tnfa					1233:1236	Tnfa	1233:1236	Tnfa	1233:1236	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	80	theme	tumor	1208:1212	arg1	α					1230:1230	tumor necrosis factor α	1208:1230	tumor necrosis factor α (Tnfa)	1208:1237	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	4	81	theme	%	748:748	arg1	adenine					750:756	0.25% adenine	744:756	0.25% adenine	744:756	Methods Male 7-wk-old ICR mice were fed an AIN-93G diet that contained 0.25% adenine for 2 wk to induce CKD.					
29659957	7	82	theme	renal	1179:1183	arg1	cytokines					1198:1206	renal inflammatory cytokines	1179:1206	renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6	1179:1275	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	8	83	from	2.2-	1430:1433	arg1	kidney					1541:1546	the kidney	1537:1546	the kidney	1537:1546	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	84	theme	CKD+GG	1395:1400	arg1	groups					1402:1407	the CON, CKD+PHGG, and CKD+GG groups	1372:1407	groups	1402:1407	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	3	85	dep	substances	528:537	arg1	i.e.					522:525	i.e.	522:525	i.e.	522:525	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	9	86	theme	greater	1676:1682	arg1	expression					1684:1693	greater expression	1676:1693	greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold)	1676:1861	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	86	theme	greater	1676:1682	arg1	2.1-					1845:1848	2.1- to 2.6-fold	1845:1860	2.1- to 2.6-fold	1845:1860	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	10	87	theme	Supplemental	1987:1998	arg1	feeding					2000:2006	Conclusion Supplemental feeding	1976:2006	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG,	1976:2049	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	3	88	theme	gum	476:478	arg1	effects					411:417	the effects	407:417	the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development	407:597	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	8	89	theme	CKD	1414:1416	arg1	group					1418:1422	the CKD group	1410:1422	the CKD group	1410:1422	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	1	90	theme	Chronic	117:123	arg1	disease					132:138	Background Chronic kidney disease	106:138	Background Chronic kidney disease (CKD)	106:144	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	1	90	theme	Chronic	117:123	arg1	problem					168:174	a worldwide health problem	149:174	a worldwide health problem	149:174	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	1	90	theme	Chronic	117:123	arg1	CKD					141:143	CKD	141:143	CKD	141:143	Background Chronic kidney disease (CKD) is a worldwide health problem.					
29659957	7	91	theme	short-chain	1288:1298	arg1	SCFAs					1313:1317	SCFAs	1313:1317	SCFAs	1313:1317	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	91	theme	short-chain	1288:1298	arg1	acids					1306:1310	cecal short-chain fatty acids	1282:1310	cecal short-chain fatty acids (SCFAs)	1282:1318	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	8	92	dep	115-fold	1624:1631	arg1	to					1621:1622	to	1621:1622	to	1621:1622	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	7	93	theme	molecule	1138:1145	arg1	proteins					1069:1076	the colonic TJ proteins	1054:1076	the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms	1054:1176	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	93	theme	molecule	1138:1145	arg1	JAMA					1150:1153	JAMA	1150:1153	JAMA	1150:1153	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	93	theme	molecule	1138:1145	arg1	A					1147:1147	junctional adhesion molecule A	1118:1147	junctional adhesion molecule A (JAMA)	1118:1154	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	94	theme	TJ	1066:1067	arg1	proteins					1069:1076	the colonic TJ proteins	1054:1076	the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms	1054:1176	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	94	theme	TJ	1066:1067	arg1	occludin					1108:1115	occludin	1108:1115	occludin	1108:1115	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	94	theme	TJ	1066:1067	arg1	occludens					1085:1093	zonula occludens (ZO) 1	1078:1100	zonula occludens (ZO) 1	1078:1100	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	94	theme	TJ	1066:1067	arg1	A					1147:1147	junctional adhesion molecule A	1118:1147	junctional adhesion molecule A (JAMA)	1118:1154	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	94	theme	TJ	1066:1067	arg1	ZO2					1103:1105	ZO2	1103:1105	ZO2	1103:1105	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	95	theme	Plasma	1027:1032	arg1	concentration					1039:1051	Plasma urea concentration	1027:1051	Plasma urea concentration	1027:1051	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	3	96	theme	distinct	544:551	arg1	characteristics					563:577	distinct viscosity characteristics	544:577	distinct viscosity characteristics	544:577	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	7	97	theme	junctional	1118:1127	arg1	proteins					1069:1076	the colonic TJ proteins	1054:1076	the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms	1054:1176	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	97	theme	junctional	1118:1127	arg1	JAMA					1150:1153	JAMA	1150:1153	JAMA	1150:1153	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	97	theme	junctional	1118:1127	arg1	A					1147:1147	junctional adhesion molecule A	1118:1147	junctional adhesion molecule A (JAMA)	1118:1154	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	8	98	theme	plasma	1454:1459	arg1	concentration					1466:1478	4.4-fold higher plasma urea concentration	1438:1478	4.4-fold higher plasma urea concentration	1438:1478	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	3	99	from	effects	411:417	arg1	development					587:597	CKD development	583:597	CKD development	583:597	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	9	100	theme	CKD	1965:1967	arg1	group					1969:1973	the CKD group	1961:1973	the CKD group	1961:1973	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	3	101	dep	Objective	381:389	arg1	investigated					394:405	investigated	394:405	investigated	394:405	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	4	102	theme	Male	681:684	arg1	mice					699:702	Male 7-wk-old ICR mice	681:702	Male 7-wk-old ICR mice	681:702	Methods Male 7-wk-old ICR mice were fed an AIN-93G diet that contained 0.25% adenine for 2 wk to induce CKD.					
29659957	3	103	with	substances	528:537	arg1	characteristics					563:577	distinct viscosity characteristics	544:577	distinct viscosity characteristics	544:577	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	8	104	theme	greater	1484:1490	arg1	expression					1492:1501	greater expression	1484:1501	greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold)	1484:1632	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	9	105	theme	CON	1639:1641	arg1	CKD+PHGG					1644:1651	The CON, CKD+PHGG, and CKD+GG groups	1635:1670	CKD+PHGG	1644:1651	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	4	106	theme	ICR	695:697	arg1	mice					699:702	Male 7-wk-old ICR mice	681:702	Male 7-wk-old ICR mice	681:702	Methods Male 7-wk-old ICR mice were fed an AIN-93G diet that contained 0.25% adenine for 2 wk to induce CKD.					
29659957	3	107	theme	dietary	438:444	arg1	DFs					454:456	DFs	454:456	DFs	454:456	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	3	107	theme	dietary	438:444	arg1	fibers					446:451	the fermentable dietary fibers	422:451	the fermentable dietary fibers (DFs)	422:457	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	0	108	theme	Fermentable	8:18	arg1	Fibers					20:25	Dietary Fermentable Fibers	0:25	Dietary Fermentable Fibers	0:25	Dietary Fermentable Fibers Attenuate Chronic Kidney Disease in Mice by Protecting the Intestinal Barrier.					
29659957	2	109	theme	CKD	364:366	arg1	development					368:378	CKD development	364:378	CKD development	364:378	Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development.					
29659957	8	110	theme	cytokine	1519:1526	arg1	Tnfa					1559:1562	Tnfa	1559:1562	Tnfa (4.4- to 48-fold)	1559:1580	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	110	theme	cytokine	1519:1526	arg1	Il1b					1583:1586	Il1b	1583:1586	Il1b (4.6- to 56-fold)	1583:1604	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	110	theme	cytokine	1519:1526	arg1	Il6					1611:1613	Il6	1611:1613	Il6 (8.8- to 115-fold)	1611:1632	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	110	theme	cytokine	1519:1526	arg1	genes					1528:1532	inflammatory cytokine genes	1506:1532	inflammatory cytokine genes	1506:1532	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	8	111	dep	Il1b	1583:1586	arg1	4.6-					1589:1592	4.6-	1589:1592	4.6- to 56-fold	1589:1603	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
29659957	0	112	theme	Kidney	45:50	arg1	Disease					52:58	Chronic Kidney Disease	37:58	Chronic Kidney Disease in Mice	37:66	Dietary Fermentable Fibers Attenuate Chronic Kidney Disease in Mice by Protecting the Intestinal Barrier.					
29659957	9	113	dep	3.0-	1786:1789	arg1	to					1791:1792	to	1791:1792	to	1791:1792	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	5	114	theme	10	890:891	arg1	%					892:892	%	892:892	%	892:892	Mice fed adenine were then divided into 3 groups and fed the unsupplemented diet (CKD) or a diet containing 10% PHGG (CKD+PHGG) or GG (CKD+GG) for 3 wk.					
29659957	7	115	theme	zonula	1078:1083	arg1	proteins					1069:1076	the colonic TJ proteins	1054:1076	the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms	1054:1176	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	115	theme	zonula	1078:1083	arg1	ZO					1096:1097	ZO	1096:1097	ZO	1096:1097	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	7	115	theme	zonula	1078:1083	arg1	occludens					1085:1093	zonula occludens (ZO) 1	1078:1100	zonula occludens (ZO) 1	1078:1100	Plasma urea concentration; the colonic TJ proteins zonula occludens (ZO) 1, ZO2, occludin, junctional adhesion molecule A (JAMA), and claudin isoforms; renal inflammatory cytokines tumor necrosis factor α (Tnfa), interleukin (Il ) 1β (Il1b), and Il6; and cecal short-chain fatty acids (SCFAs) and microflora were analyzed.					
29659957	9	116	theme	TJ	1706:1707	arg1	JAMA					1805:1808	JAMA	1805:1808	JAMA (4.4- to 5.4-fold)	1805:1827	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	116	theme	TJ	1706:1707	arg1	claudin					1834:1840	claudin 7	1834:1842	claudin 7	1834:1842	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	116	theme	TJ	1706:1707	arg1	proteins					1709:1716	colonic TJ proteins	1698:1716	colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7	1698:1842	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	116	theme	TJ	1706:1707	arg1	ZO1					1728:1730	ZO1	1728:1730	ZO1 (2.9- to 3.7-fold)	1728:1749	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	116	theme	TJ	1706:1707	arg1	ZO2					1752:1754	ZO2	1752:1754	ZO2 (3.4- to 4.3-fold)	1752:1773	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	116	theme	TJ	1706:1707	arg1	occludin					1776:1783	occludin	1776:1783	occludin (3.0- to 3.3-fold)	1776:1802	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	3	117	theme	particular	607:616	arg1	focus					618:622	a particular focus	605:622	a particular focus on colonic tight junction (TJ) barriers in mice	605:670	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	2	118	theme	impaired	321:328	arg1	barrier					341:347	an impaired intestinal barrier	318:347	an impaired intestinal barrier	318:347	Although the pathogenesis of CKD is still unclear, recent studies suggest that systemic inflammation caused by a dysregulated microflora and an impaired intestinal barrier is involved in CKD development.					
29659957	6	119	theme	Control	935:941	arg1	mice					949:952	Control (CON) mice	935:952	Control (CON) mice	935:952	Control (CON) mice were fed an AIN-93G diet without adenine throughout the 5-wk experiment.					
29659957	10	120	theme	colonic	2123:2129	arg1	integrity					2139:2147	colonic barrier integrity	2123:2147	colonic barrier integrity	2123:2147	Conclusion Supplemental feeding with fermentable DFs, such as GG and PHGG, might be effective for the prevention or management of CKD by restoring colonic barrier integrity and microflora composition, as shown in mice.					
29659957	3	121	theme	unmodified	460:469	arg1	GG					481:482	GG	481:482	GG	481:482	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	3	121	theme	unmodified	460:469	arg1	gum					476:478	unmodified guar gum	460:478	unmodified guar gum (GG)	460:483	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	9	122	dep	3.5-fold	1894:1901	arg1	to					1891:1892	to	1891:1892	to	1891:1892	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	3	123	theme	tight	635:639	arg1	TJ					651:652	TJ	651:652	TJ	651:652	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	3	123	theme	tight	635:639	arg1	junction					641:648	colonic tight junction	627:648	colonic tight junction (TJ) barriers in mice	627:670	Objective We investigated the effects of the fermentable dietary fibers (DFs), unmodified guar gum (GG), and partially hydrolyzed GG (PHGG) (i.e., substances with distinct viscosity characteristics) on CKD development, with a particular focus on colonic tight junction (TJ) barriers in mice.					
29659957	9	124	theme	2.6-fold	1853:1860	arg1	expression					1684:1693	greater expression	1676:1693	greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold)	1676:1861	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	9	124	theme	2.6-fold	1853:1860	arg1	2.1-					1845:1848	2.1- to 2.6-fold	1845:1860	2.1- to 2.6-fold	1845:1860	The CON, CKD+PHGG, and CKD+GG groups had greater expression of colonic TJ proteins including ZO1 (2.9- to 3.7-fold), ZO2 (3.4- to 4.3-fold), occludin (3.0- to 3.3-fold), JAMA (4.4- to 5.4-fold), and claudin 7 (2.1- to 2.6-fold) and higher cecal SCFA (1.8- to 3.5-fold) and Lactobacillus (2.7- to 4.0-fold) concentrations than the CKD group.					
29659957	8	125	theme	CON	1376:1378	arg1	CKD+PHGG					1381:1388	the CON, CKD+PHGG, and CKD+GG groups	1372:1407	CKD+PHGG	1381:1388	Results Compared with the CON, CKD+PHGG, and CKD+GG groups, the CKD group had a 2.2- to 4.4-fold higher plasma urea concentration and greater expression of inflammatory cytokine genes in the kidney, including Tnfa (4.4- to 48-fold), Il1b (4.6- to 56-fold), and Il6 (8.8- to 115-fold).					
31504685	2	0	theme	volatile	491:498	arg1	concentrations					517:530	volatile fatty acid (VFA) concentrations	491:530	volatile fatty acid (VFA) concentrations	491:530	OBJECTIVES We investigated the impact of feeding mode and prebiotics on bacterial colonization and volatile fatty acid (VFA) concentrations.					
31504685	1	1	theme	gut	322:324	arg1	microbiota					326:335	the gut microbiota	318:335	the gut microbiota	318:335	BACKGROUND Many infants consume both human milk and infant formula (combination-fed); however, little is known about how combination-feeding affects the gut microbiota or prebiotic fermentation compared to formula feeding.					
31504685	9	2	theme	pyrosequencing	1257:1270	arg1	data					1272:1275	DGGE and pyrosequencing data	1248:1275	DGGE and pyrosequencing data separated microbiota of FF from CF and SR	1248:1317	RESULTS Distance-based redundancy analysis of DGGE and pyrosequencing data separated microbiota of FF from CF and SR.					
31504685	8	3	theme	gas	1182:1184	arg1	chromatography					1186:1199	gas chromatography	1182:1199	gas chromatography	1182:1199	VFAs were determined by gas chromatography.					
31504685	3	4	with	formula-fed	652:662	arg1	FP					681:682	FP	681:682	FP	681:682	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	3	4	with	formula-fed	652:662	arg1	prebiotics					669:678	prebiotics	669:678	prebiotics (FP)	669:683	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	3	5	dep	formula-fed	634:644	arg1	FF					647:648	FF	647:648	FF	647:648	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	16	6	theme	bacterial	1988:1996	arg1	composition					1998:2008	piglet bacterial composition	1981:2008	piglet bacterial composition	1981:2008	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	15	7	theme	Microbial	1843:1851	arg1	patterns					1866:1873	CONCLUSIONS Microbial colonization patterns	1831:1873	CONCLUSIONS Microbial colonization patterns	1831:1873	CONCLUSIONS Microbial colonization patterns and VFA profiles of CF piglets were more similar to SR piglets than FF piglets.					
31504685	16	8	theme	piglet	1981:1986	arg1	composition					1998:2008	piglet bacterial composition	1981:2008	piglet bacterial composition	1981:2008	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	1	9	dep	formula	228:234	arg1	combination-fed					237:251	combination-fed	237:251	combination-fed	237:251	BACKGROUND Many infants consume both human milk and infant formula (combination-fed); however, little is known about how combination-feeding affects the gut microbiota or prebiotic fermentation compared to formula feeding.					
31504685	0	10	theme	Fatty	86:90	arg1	Concentrations					97:110	Volatile Fatty Acid Concentrations	77:110	Volatile Fatty Acid Concentrations	77:110	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	4	11	theme	sows	796:799	arg1	h/d					804:806	the sows 24 h/d	792:806	the sows 24 h/d	792:806	SR piglets remained with the sows 24 h/d.					
31504685	3	12	theme	Newborn	541:547	arg1	piglets					549:555	Newborn piglets	541:555	Newborn piglets (Large White and Landrace)	541:582	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	9	13	theme	FF	1301:1302	arg1	microbiota					1287:1296	microbiota	1287:1296	microbiota of FF	1287:1302	RESULTS Distance-based redundancy analysis of DGGE and pyrosequencing data separated microbiota of FF from CF and SR.					
31504685	0	14	theme	Volatile	77:84	arg1	Concentrations					97:110	Volatile Fatty Acid Concentrations	77:110	Volatile Fatty Acid Concentrations	77:110	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	1	15	theme	prebiotic	340:348	arg1	fermentation					350:361	prebiotic fermentation	340:361	prebiotic fermentation	340:361	BACKGROUND Many infants consume both human milk and infant formula (combination-fed); however, little is known about how combination-feeding affects the gut microbiota or prebiotic fermentation compared to formula feeding.					
31504685	14	16	theme	prebiotic	1804:1812	arg1	supplementation					1814:1828	prebiotic supplementation	1804:1828	prebiotic supplementation	1804:1828	Neither microbiota nor VFA profile was affected by prebiotic supplementation.					
31504685	3	17	with	formula-fed	634:644	arg1	prebiotics					750:759	prebiotics	750:759	prebiotics (CP)	750:764	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	3	17	with	formula-fed	634:644	arg1	CP					762:763	CP	762:763	CP	762:763	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	7	18	theme	gel	1130:1132	arg1	DGGE					1151:1154	DGGE	1151:1154	DGGE	1151:1154	The microbiota was analyzed by pyrosequencing and denaturing gradient gel electrophoresis (DGGE).					
31504685	7	18	theme	gel	1130:1132	arg1	electrophoresis					1134:1148	denaturing gradient gel electrophoresis	1110:1148	denaturing gradient gel electrophoresis (DGGE)	1110:1155	The microbiota was analyzed by pyrosequencing and denaturing gradient gel electrophoresis (DGGE).					
31504685	16	19	theme	relative	2036:2043	arg1	composition					1998:2008	piglet bacterial composition	1981:2008	piglet bacterial composition	1981:2008	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	3	20	dep	combination-fed	703:717	arg1	CF					720:721	CF	720:721	CF	720:721	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	13	21	dep	greater	1719:1725	arg1	P < 0.05					1728:1735	P < 0.05	1728:1735	P < 0.05	1728:1735	VFA concentrations were similar between CF and SR, while isovalerate and isobutyrate were 2-fold greater (P < 0.05) in CF than FF.					
31504685	0	22	theme	Acid	92:95	arg1	Concentrations					97:110	Volatile Fatty Acid Concentrations	77:110	Volatile Fatty Acid Concentrations	77:110	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	6	23	theme	colon	1019:1023	arg1	contents					1025:1032	formula-feeding every 12 h. Ascending colon contents	981:1032	formula-feeding every 12 h. Ascending colon contents	981:1032	CF and CP were sow-reared for 5 d and then rotated between the sow and formula-feeding every 12 h. Ascending colon contents were collected at day 21.					
31504685	16	24	theme	feeding	2057:2063	arg1	CF					2079:2080	CF	2079:2080	CF	2079:2080	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	16	24	theme	feeding	2057:2063	arg1	FF					2072:2073	FF	2072:2073	FF	2072:2073	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	16	24	theme	feeding	2057:2063	arg1	modes					2065:2069	the main feeding modes	2048:2069	the main feeding modes (FF and CF)	2048:2081	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	1	25	theme	BACKGROUND	169:178	arg1	infants					185:191	BACKGROUND Many infants	169:191	BACKGROUND Many infants	169:191	BACKGROUND Many infants consume both human milk and infant formula (combination-fed); however, little is known about how combination-feeding affects the gut microbiota or prebiotic fermentation compared to formula feeding.					
31504685	2	26	theme	acid	506:509	arg1	concentrations					517:530	volatile fatty acid (VFA) concentrations	491:530	volatile fatty acid (VFA) concentrations	491:530	OBJECTIVES We investigated the impact of feeding mode and prebiotics on bacterial colonization and volatile fatty acid (VFA) concentrations.					
31504685	6	27	theme	Ascending	1009:1017	arg1	colon					1019:1023	every 12 h. Ascending colon	997:1023	formula-feeding every 12 h. Ascending colon contents	981:1032	CF and CP were sow-reared for 5 d and then rotated between the sow and formula-feeding every 12 h. Ascending colon contents were collected at day 21.					
31504685	5	28	from	g/L	889:891	arg1	ratio					902:906	a 4:1 ratio	896:906	a 4:1 ratio	896:906	FF and FP were fed formula or formula with galactooligosaccharide and inulin (4 g/L in a 4:1 ratio).					
31504685	1	29	theme	Many	180:183	arg1	infants					185:191	BACKGROUND Many infants	169:191	BACKGROUND Many infants	169:191	BACKGROUND Many infants consume both human milk and infant formula (combination-fed); however, little is known about how combination-feeding affects the gut microbiota or prebiotic fermentation compared to formula feeding.					
31504685	0	30	theme	Feeding	0:6	arg1	Mode					8:11	Feeding Mode	0:11	Feeding Mode	0:11	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	4	31	theme	SR	767:768	arg1	piglets					770:776	SR piglets	767:776	SR piglets	767:776	SR piglets remained with the sows 24 h/d.					
31504685	6	32	theme	h.	1006:1007	arg1	colon					1019:1023	every 12 h. Ascending colon	997:1023	formula-feeding every 12 h. Ascending colon contents	981:1032	CF and CP were sow-reared for 5 d and then rotated between the sow and formula-feeding every 12 h. Ascending colon contents were collected at day 21.					
31504685	17	33	theme	FF	2170:2171	arg1	neonates					2173:2180	FF neonates	2170:2180	FF neonates	2170:2180	Thus, partial exposure to breast milk can be beneficial for microbiota development of FF neonates.					
31504685	0	34	theme	Sow-Reared	115:124	arg1	Piglets					160:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	11	35	theme	Bacterial	1396:1404	arg1	composition					1406:1416	Bacterial composition	1396:1416	Bacterial composition of CF	1396:1422	Bacterial composition of CF was more similar to SR than FF.					
31504685	14	36	theme	VFA	1776:1778	arg1	profile					1780:1786	VFA profile	1776:1786	VFA profile	1776:1786	Neither microbiota nor VFA profile was affected by prebiotic supplementation.					
31504685	15	37	theme	FF	1943:1944	arg1	piglets					1946:1952	FF piglets	1943:1952	FF piglets	1943:1952	CONCLUSIONS Microbial colonization patterns and VFA profiles of CF piglets were more similar to SR piglets than FF piglets.					
31504685	15	38	theme	colonization	1853:1864	arg1	patterns					1866:1873	CONCLUSIONS Microbial colonization patterns	1831:1873	CONCLUSIONS Microbial colonization patterns	1831:1873	CONCLUSIONS Microbial colonization patterns and VFA profiles of CF piglets were more similar to SR piglets than FF piglets.					
31504685	17	39	theme	neonates	2173:2180	arg1	development					2155:2165	microbiota development	2144:2165	microbiota development of FF neonates	2144:2180	Thus, partial exposure to breast milk can be beneficial for microbiota development of FF neonates.					
31504685	16	40	dep	modes	2065:2069	arg1	CF					2079:2080	CF	2079:2080	CF	2079:2080	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	16	40	dep	modes	2065:2069	arg1	FF					2072:2073	FF	2072:2073	FF	2072:2073	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	16	40	dep	modes	2065:2069	arg1	modes					2065:2069	the main feeding modes	2048:2069	the main feeding modes (FF and CF)	2048:2081	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	1	41	theme	formula	375:381	arg1	feeding					383:389	formula feeding	375:389	formula feeding	375:389	BACKGROUND Many infants consume both human milk and infant formula (combination-fed); however, little is known about how combination-feeding affects the gut microbiota or prebiotic fermentation compared to formula feeding.					
31504685	15	42	theme	CONCLUSIONS	1831:1841	arg1	patterns					1866:1873	CONCLUSIONS Microbial colonization patterns	1831:1873	CONCLUSIONS Microbial colonization patterns	1831:1873	CONCLUSIONS Microbial colonization patterns and VFA profiles of CF piglets were more similar to SR piglets than FF piglets.					
31504685	9	43	theme	Distance-based	1210:1223	arg1	analysis					1236:1243	Distance-based redundancy analysis	1210:1243	Distance-based redundancy analysis of DGGE and pyrosequencing data separated microbiota of FF from CF and SR	1210:1317	RESULTS Distance-based redundancy analysis of DGGE and pyrosequencing data separated microbiota of FF from CF and SR.					
31504685	0	44	theme	Formula-Fed	127:137	arg1	Piglets					160:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	0	45	from	Composition	61:71	arg1	Piglets					160:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	5	46	theme	4:1	898:900	arg1	ratio					902:906	a 4:1 ratio	896:906	a 4:1 ratio	896:906	FF and FP were fed formula or formula with galactooligosaccharide and inulin (4 g/L in a 4:1 ratio).					
31504685	12	47	theme	bacterial	1459:1467	arg1	genera					1469:1474	No bacterial genera	1456:1474	No bacterial genera in CF	1456:1480	No bacterial genera in CF significantly differed from SR; however, 9 genera differed between CF and FF, including Lactobacillus, Clostridium XIVa, and Fusobacterium.					
31504685	1	48	theme	human	206:210	arg1	milk					212:215	human milk	206:215	human milk	206:215	BACKGROUND Many infants consume both human milk and infant formula (combination-fed); however, little is known about how combination-feeding affects the gut microbiota or prebiotic fermentation compared to formula feeding.					
31504685	2	49	theme	bacterial	464:472	arg1	colonization					474:485	bacterial colonization	464:485	bacterial colonization	464:485	OBJECTIVES We investigated the impact of feeding mode and prebiotics on bacterial colonization and volatile fatty acid (VFA) concentrations.					
31504685	9	50	theme	redundancy	1225:1234	arg1	analysis					1236:1243	Distance-based redundancy analysis	1210:1243	Distance-based redundancy analysis of DGGE and pyrosequencing data separated microbiota of FF from CF and SR	1210:1317	RESULTS Distance-based redundancy analysis of DGGE and pyrosequencing data separated microbiota of FF from CF and SR.					
31504685	15	51	theme	SR	1927:1928	arg1	piglets					1930:1936	SR piglets	1927:1936	SR piglets	1927:1936	CONCLUSIONS Microbial colonization patterns and VFA profiles of CF piglets were more similar to SR piglets than FF piglets.					
31504685	16	52	theme	VFA	2017:2019	arg1	concentrations					2021:2034	VFA concentrations	2017:2034	VFA concentrations	2017:2034	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	2	53	theme	fatty	500:504	arg1	VFA					512:514	VFA	512:514	VFA	512:514	OBJECTIVES We investigated the impact of feeding mode and prebiotics on bacterial colonization and volatile fatty acid (VFA) concentrations.					
31504685	2	53	theme	fatty	500:504	arg1	acid					506:509	fatty acid	500:509	volatile fatty acid (VFA) concentrations	491:530	OBJECTIVES We investigated the impact of feeding mode and prebiotics on bacterial colonization and volatile fatty acid (VFA) concentrations.					
31504685	13	54	theme	VFA	1622:1624	arg1	concentrations					1626:1639	VFA concentrations	1622:1639	VFA concentrations	1622:1639	VFA concentrations were similar between CF and SR, while isovalerate and isobutyrate were 2-fold greater (P < 0.05) in CF than FF.					
31504685	6	55	theme	formula-feeding	981:995	arg1	contents					1025:1032	formula-feeding every 12 h. Ascending colon contents	981:1032	formula-feeding every 12 h. Ascending colon contents	981:1032	CF and CP were sow-reared for 5 d and then rotated between the sow and formula-feeding every 12 h. Ascending colon contents were collected at day 21.					
31504685	12	56	from	genera	1469:1474	arg1	CF					1479:1480	CF	1479:1480	CF	1479:1480	No bacterial genera in CF significantly differed from SR; however, 9 genera differed between CF and FF, including Lactobacillus, Clostridium XIVa, and Fusobacterium.					
31504685	16	57	theme	main	2052:2055	arg1	CF					2079:2080	CF	2079:2080	CF	2079:2080	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	16	57	theme	main	2052:2055	arg1	FF					2072:2073	FF	2072:2073	FF	2072:2073	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	16	57	theme	main	2052:2055	arg1	modes					2065:2069	the main feeding modes	2048:2069	the main feeding modes (FF and CF)	2048:2081	Prebiotics did not affect piglet bacterial composition and/or VFA concentrations relative to the main feeding modes (FF and CF).					
31504685	0	58	from	Concentrations	97:110	arg1	Piglets					160:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	11	59	theme	CF	1421:1422	arg1	composition					1406:1416	Bacterial composition	1396:1416	Bacterial composition of CF	1396:1422	Bacterial composition of CF was more similar to SR than FF.					
31504685	3	60	dep	METHODS	533:539	arg1	combination-fed					729:743	combination-fed	729:743	combination-fed	729:743	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	3	60	dep	METHODS	533:539	arg1	combination-fed					703:717	combination-fed	703:717	combination-fed	703:717	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	3	60	dep	METHODS	533:539	arg1	formula-fed					652:662	formula-fed	652:662	formula-fed	652:662	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	3	60	dep	METHODS	533:539	arg1	assigned					598:605	assigned	598:605	were randomly assigned to 5 groups (n = 6/group)	584:631	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	3	60	dep	METHODS	533:539	arg1	sow-reared					686:695	sow-reared	686:695	sow-reared	686:695	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	3	60	dep	METHODS	533:539	arg1	formula-fed					634:644	formula-fed	634:644	formula-fed	634:644	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	9	61	theme	DGGE	1248:1251	arg1	data					1272:1275	DGGE and pyrosequencing data	1248:1275	DGGE and pyrosequencing data separated microbiota of FF from CF and SR	1248:1317	RESULTS Distance-based redundancy analysis of DGGE and pyrosequencing data separated microbiota of FF from CF and SR.					
31504685	17	62	theme	partial	2090:2096	arg1	exposure					2098:2105	partial exposure	2090:2105	partial exposure to breast milk	2090:2120	Thus, partial exposure to breast milk can be beneficial for microbiota development of FF neonates.					
31504685	15	63	theme	piglets	1898:1904	arg1	patterns					1866:1873	CONCLUSIONS Microbial colonization patterns	1831:1873	CONCLUSIONS Microbial colonization patterns	1831:1873	CONCLUSIONS Microbial colonization patterns and VFA profiles of CF piglets were more similar to SR piglets than FF piglets.					
31504685	15	63	theme	piglets	1898:1904	arg1	profiles					1883:1890	VFA profiles	1879:1890	VFA profiles of CF piglets	1879:1904	CONCLUSIONS Microbial colonization patterns and VFA profiles of CF piglets were more similar to SR piglets than FF piglets.					
31504685	5	64	dep	galactooligosaccharide	852:873	arg1	g/L					889:891	4 g/L	887:891	4 g/L in a 4:1 ratio	887:906	FF and FP were fed formula or formula with galactooligosaccharide and inulin (4 g/L in a 4:1 ratio).					
31504685	17	65	theme	breast	2110:2115	arg1	milk					2117:2120	breast milk	2110:2120	breast milk	2110:2120	Thus, partial exposure to breast milk can be beneficial for microbiota development of FF neonates.					
31504685	0	66	theme	Combination-Fed	144:158	arg1	Piglets					160:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Sow-Reared, Formula-Fed, and Combination-Fed Piglets	115:166	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	15	67	theme	VFA	1879:1881	arg1	profiles					1883:1890	VFA profiles	1879:1890	VFA profiles of CF piglets	1879:1904	CONCLUSIONS Microbial colonization patterns and VFA profiles of CF piglets were more similar to SR piglets than FF piglets.					
31504685	17	68	theme	microbiota	2144:2153	arg1	development					2155:2165	microbiota development	2144:2165	microbiota development of FF neonates	2144:2180	Thus, partial exposure to breast milk can be beneficial for microbiota development of FF neonates.					
31504685	3	69	dep	piglets	549:555	arg1	Landrace					574:581	Landrace	574:581	Landrace	574:581	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	3	69	dep	piglets	549:555	arg1	White					564:568	White	564:568	White	564:568	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	1	70	theme	infant	221:226	arg1	formula					228:234	infant formula	221:234	infant formula (combination-fed)	221:252	BACKGROUND Many infants consume both human milk and infant formula (combination-fed); however, little is known about how combination-feeding affects the gut microbiota or prebiotic fermentation compared to formula feeding.					
31504685	0	71	theme	Microbiota	50:59	arg1	Composition					61:71	Colonic Microbiota Composition	42:71	Colonic Microbiota Composition	42:71	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	2	72	dep	OBJECTIVES	392:401	arg1	investigated					406:417	investigated	406:417	investigated the impact of feeding mode and prebiotics on bacterial colonization and volatile fatty acid (VFA) concentrations	406:530	OBJECTIVES We investigated the impact of feeding mode and prebiotics on bacterial colonization and volatile fatty acid (VFA) concentrations.					
31504685	12	73	theme	Clostridium	1585:1595	arg1	XIVa					1597:1600	Clostridium XIVa	1585:1600	Clostridium XIVa	1585:1600	No bacterial genera in CF significantly differed from SR; however, 9 genera differed between CF and FF, including Lactobacillus, Clostridium XIVa, and Fusobacterium.					
31504685	9	74	theme	data	1272:1275	arg1	analysis					1236:1243	Distance-based redundancy analysis	1210:1243	Distance-based redundancy analysis of DGGE and pyrosequencing data separated microbiota of FF from CF and SR	1210:1317	RESULTS Distance-based redundancy analysis of DGGE and pyrosequencing data separated microbiota of FF from CF and SR.					
31504685	0	75	theme	Colonic	42:48	arg1	Composition					61:71	Colonic Microbiota Composition	42:71	Colonic Microbiota Composition	42:71	Feeding Mode, but Not Prebiotics, Affects Colonic Microbiota Composition and Volatile Fatty Acid Concentrations in Sow-Reared, Formula-Fed, and Combination-Fed Piglets.					
31504685	3	76	dep	sow-reared	686:695	arg1	SR					698:699	SR	698:699	SR	698:699	METHODS Newborn piglets (Large White and Landrace) were randomly assigned to 5 groups (n = 6/group): formula-fed (FF), formula-fed with prebiotics (FP), sow-reared (SR), combination-fed (CF), and combination-fed with prebiotics (CP).					
31504685	15	77	theme	CF	1895:1896	arg1	piglets					1898:1904	CF piglets	1895:1904	CF piglets	1895:1904	CONCLUSIONS Microbial colonization patterns and VFA profiles of CF piglets were more similar to SR piglets than FF piglets.					
31504685	7	78	theme	gradient	1121:1128	arg1	DGGE					1151:1154	DGGE	1151:1154	DGGE	1151:1154	The microbiota was analyzed by pyrosequencing and denaturing gradient gel electrophoresis (DGGE).					
31504685	7	78	theme	gradient	1121:1128	arg1	electrophoresis					1134:1148	denaturing gradient gel electrophoresis	1110:1148	denaturing gradient gel electrophoresis (DGGE)	1110:1155	The microbiota was analyzed by pyrosequencing and denaturing gradient gel electrophoresis (DGGE).					
31504685	7	79	theme	denaturing	1110:1119	arg1	DGGE					1151:1154	DGGE	1151:1154	DGGE	1151:1154	The microbiota was analyzed by pyrosequencing and denaturing gradient gel electrophoresis (DGGE).					
31504685	7	79	theme	denaturing	1110:1119	arg1	electrophoresis					1134:1148	denaturing gradient gel electrophoresis	1110:1148	denaturing gradient gel electrophoresis (DGGE)	1110:1155	The microbiota was analyzed by pyrosequencing and denaturing gradient gel electrophoresis (DGGE).					
29717804	6	0	theme	commercial	891:900	arg1	printer					905:911	a commercial 3D printer	889:911	a commercial 3D printer based on fused deposition modelling	889:947	3D printed scaffolds were manufactured with a commercial 3D printer based on fused deposition modelling.					
29717804	10	1	theme	proposed	1457:1464	arg1	scaffolds					1466:1474	the proposed scaffolds	1453:1474	the proposed scaffolds	1453:1474	On the other hand, the proliferation of sheep bone marrow cells on the proposed scaffolds was evaluated over 8 days.					
29717804	7	2	theme	porosity	1090:1097	arg1	values					1099:1104	450 and 500 µm and porosity values	1071:1104	450 and 500 µm and porosity values between 50 and 60%	1071:1123	The operation conditions have been established in order to obtain scaffolds with a 0/90° pattern with pore sizes between 450 and 500 µm and porosity values between 50 and 60%.					
29717804	1	3	theme	biological	270:279	arg1	properties					281:290	improved mechanical and biological properties	246:290	improved mechanical and biological properties	246:290	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	7	4	theme	µm	1083:1084	arg1	values					1099:1104	450 and 500 µm and porosity values	1071:1104	450 and 500 µm and porosity values between 50 and 60%	1071:1123	The operation conditions have been established in order to obtain scaffolds with a 0/90° pattern with pore sizes between 450 and 500 µm and porosity values between 50 and 60%.					
29717804	2	5	theme	mechanical	307:316	arg1	behavior					318:325	the mechanical behavior	303:325	the mechanical behavior	303:325	Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering.					
29717804	9	6	theme	reinforcement	1364:1376	arg1	effect					1378:1383	this reinforcement effect	1359:1383	this reinforcement effect	1359:1383	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	2	7	theme	Improving	293:301	arg1	behavior					318:325	the mechanical behavior	303:325	the mechanical behavior	303:325	Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering.					
29717804	5	8	theme	composites	833:842	arg1	properties					815:824	the thermal properties	803:824	the thermal properties of the composites	803:842	These combinations were subjected to a thermogravimetric analysis in order to evaluate the influence of the additive in the thermal properties of the composites.					
29717804	6	9	theme	printed	848:854	arg1	scaffolds					856:864	3D printed scaffolds	845:864	3D printed scaffolds	845:864	3D printed scaffolds were manufactured with a commercial 3D printer based on fused deposition modelling.					
29717804	8	10	theme	flexural	1209:1216	arg1	modes					1218:1222	flexural modes	1209:1222	flexural modes	1209:1222	The mechanical properties of these structures were measured in the compression and flexural modes.					
29717804	2	11	theme	biological	335:344	arg1	performance					346:356	the biological performance	331:356	the biological performance	331:356	Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering.					
29717804	1	12	theme	three-dimensional	200:216	arg1	scaffolds					231:239	three-dimensional (3D) printed scaffolds	200:239	three-dimensional (3D) printed scaffolds with improved mechanical and biological properties	200:290	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	12	13	contain	have	1663:1666	arg1	PCL					1649:1651	PCL	1649:1651	PCL:MCC 98:2	1649:1660	Therefore, these scaffolds (PCL:MCC 98:2) have suitable properties to be further evaluated for bone tissue engineering applications.					
29717804	12	13	contain	have	1663:1666	arg1	scaffolds					1638:1646	these scaffolds	1632:1646	these scaffolds (PCL:MCC 98:2)	1632:1661	Therefore, these scaffolds (PCL:MCC 98:2) have suitable properties to be further evaluated for bone tissue engineering applications.					
29717804	12	13	contain	have	1663:1666	arg2	properties					1677:1686	suitable properties	1668:1686	suitable properties	1668:1686	Therefore, these scaffolds (PCL:MCC 98:2) have suitable properties to be further evaluated for bone tissue engineering applications.					
29717804	7	14	theme	pore	1052:1055	arg1	sizes					1057:1061	pore sizes	1052:1061	pore sizes between 450 and 500 µm and porosity values between 50 and 60%	1052:1123	The operation conditions have been established in order to obtain scaffolds with a 0/90° pattern with pore sizes between 450 and 500 µm and porosity values between 50 and 60%.					
29717804	3	15	theme	matrix	595:600	arg1	properties					573:582	the properties	569:582	the properties of the PCL matrix	569:600	Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix.					
29717804	5	16	theme	thermal	807:813	arg1	properties					815:824	the thermal properties	803:824	the thermal properties of the composites	803:842	These combinations were subjected to a thermogravimetric analysis in order to evaluate the influence of the additive in the thermal properties of the composites.					
29717804	6	17	theme	deposition	928:937	arg1	modelling					939:947	fused deposition modelling	922:947	fused deposition modelling	922:947	3D printed scaffolds were manufactured with a commercial 3D printer based on fused deposition modelling.					
29717804	1	18	theme	printed	223:229	arg1	scaffolds					231:239	three-dimensional (3D) printed scaffolds	200:239	three-dimensional (3D) printed scaffolds with improved mechanical and biological properties	200:290	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	10	19	theme	bone	1432:1435	arg1	cells					1444:1448	sheep bone marrow cells	1426:1448	sheep bone marrow cells	1426:1448	On the other hand, the proliferation of sheep bone marrow cells on the proposed scaffolds was evaluated over 8 days.					
29717804	16	20	dep	Res	1879:1881	arg1	B					1888:1888	Part B	1883:1888	J Biomed Mater Res Part B: Appl Biomater 107B: 521-528, 2019.	1864:1924	J Biomed Mater Res Part B: Appl Biomater 107B: 521-528, 2019.					
29717804	16	20	dep	Res	1879:1881	arg1	Biomater					1896:1903	Biomater	1896:1903	Biomater	1896:1903	J Biomed Mater Res Part B: Appl Biomater 107B: 521-528, 2019.					
29717804	16	20	dep	Res	1879:1881	arg1	2019					1920:1923	2019	1920:1923	2019	1920:1923	J Biomed Mater Res Part B: Appl Biomater 107B: 521-528, 2019.					
29717804	10	21	from	proliferation	1409:1421	arg1	scaffolds					1466:1474	the proposed scaffolds	1453:1474	the proposed scaffolds	1453:1474	On the other hand, the proliferation of sheep bone marrow cells on the proposed scaffolds was evaluated over 8 days.					
29717804	15	22	dep	©	1833:1833	arg1	Inc.					1859:1862	Inc.	1859:1862	Inc.	1859:1862	© 2018 Wiley Periodicals, Inc.					
29717804	7	23	theme	operation	954:962	arg1	conditions					964:973	The operation conditions	950:973	The operation conditions	950:973	The operation conditions have been established in order to obtain scaffolds with a 0/90° pattern with pore sizes between 450 and 500 µm and porosity values between 50 and 60%.					
29717804	11	24	contain	containing	1602:1611	arg1	samples					1594:1600	samples	1594:1600	samples containing 2% MCC	1594:1618	The results show that the proliferation is significantly better (p < 0.05) on the group of samples containing 2% MCC.					
29717804	11	24	contain	containing	1602:1611	arg2	MCC					1616:1618	2% MCC	1613:1618	2% MCC	1613:1618	The results show that the proliferation is significantly better (p < 0.05) on the group of samples containing 2% MCC.					
29717804	9	25	theme	%	1257:1257	arg1	MCC					1259:1261	2 and 5% MCC	1250:1261	2 and 5% MCC	1250:1261	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	4	26	from	concentrations	607:620	arg1	groups					636:641	the groups	632:641	the groups of samples	632:652	The concentrations of MCC in the groups of samples were 0, 2, 5, and 10% (w/w).					
29717804	4	26	from	concentrations	607:620	arg1	samples					646:652	samples	646:652	samples	646:652	The concentrations of MCC in the groups of samples were 0, 2, 5, and 10% (w/w).					
29717804	1	27	from	additive	146:153	arg1	matrices					181:188	polycaprolactone (PCL) matrices	158:188	polycaprolactone (PCL) matrices	158:188	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	9	28	contain	containing	1239:1248	arg2	MCC					1259:1261	2 and 5% MCC	1250:1261	2 and 5% MCC	1250:1261	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	9	28	contain	containing	1239:1248	arg1	scaffolds					1229:1237	The scaffolds	1225:1237	The scaffolds containing 2 and 5% MCC	1225:1261	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	3	29	theme	Different	476:484	arg1	samples					496:502	samples	496:502	samples	496:502	Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix.					
29717804	3	29	theme	Different	476:484	arg1	groups					486:491	Different groups	476:491	Different groups of samples	476:502	Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix.					
29717804	0	30	theme	Three-dimensional	0:16	arg1	cellulose					60:68	Three-dimensional printed polycaprolactone-microcrystalline cellulose	0:68	Three-dimensional printed polycaprolactone-microcrystalline cellulose	0:68	Three-dimensional printed polycaprolactone-microcrystalline cellulose scaffolds.					
29717804	11	31	dep	better	1560:1565	arg1	p < 0.05					1568:1575	p < 0.05	1568:1575	p < 0.05	1568:1575	The results show that the proliferation is significantly better (p < 0.05) on the group of samples containing 2% MCC.					
29717804	16	32	theme	Part	1883:1886	arg1	B					1888:1888	Part B	1883:1888	J Biomed Mater Res Part B: Appl Biomater 107B: 521-528, 2019.	1864:1924	J Biomed Mater Res Part B: Appl Biomater 107B: 521-528, 2019.					
29717804	9	33	contain	have	1263:1266	arg2	flexural					1275:1282	flexural	1275:1282	flexural	1275:1282	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	9	33	contain	have	1263:1266	arg2	modulus					1308:1314	higher flexural and compression elastic modulus	1268:1314	modulus	1308:1314	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	9	33	contain	have	1263:1266	arg1	scaffolds					1229:1237	The scaffolds	1225:1237	The scaffolds containing 2 and 5% MCC	1225:1261	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	0	34	theme	polycaprolactone-microcrystalline	26:58	arg1	cellulose					60:68	Three-dimensional printed polycaprolactone-microcrystalline cellulose	0:68	Three-dimensional printed polycaprolactone-microcrystalline cellulose	0:68	Three-dimensional printed polycaprolactone-microcrystalline cellulose scaffolds.					
29717804	9	35	theme	compression	1288:1298	arg1	modulus					1308:1314	higher flexural and compression elastic modulus	1268:1314	modulus	1308:1314	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	6	36	theme	3D	845:846	arg1	scaffolds					856:864	3D printed scaffolds	845:864	3D printed scaffolds	845:864	3D printed scaffolds were manufactured with a commercial 3D printer based on fused deposition modelling.					
29717804	7	37	with	pattern	1039:1045	arg1	sizes					1057:1061	pore sizes	1052:1061	pore sizes between 450 and 500 µm and porosity values between 50 and 60%	1052:1123	The operation conditions have been established in order to obtain scaffolds with a 0/90° pattern with pore sizes between 450 and 500 µm and porosity values between 50 and 60%.					
29717804	11	38	theme	%	1614:1614	arg1	MCC					1616:1618	2% MCC	1613:1618	2% MCC	1613:1618	The results show that the proliferation is significantly better (p < 0.05) on the group of samples containing 2% MCC.					
29717804	2	39	theme	tissue	456:461	arg1	engineering					463:473	bone tissue engineering	451:473	bone tissue engineering	451:473	Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering.					
29717804	12	40	theme	bone	1716:1719	arg1	applications					1740:1751	bone tissue engineering applications	1716:1751	bone tissue engineering applications	1716:1751	Therefore, these scaffolds (PCL:MCC 98:2) have suitable properties to be further evaluated for bone tissue engineering applications.					
29717804	10	41	theme	cells	1444:1448	arg1	proliferation					1409:1421	the proliferation	1405:1421	the proliferation of sheep bone marrow cells on the proposed scaffolds	1405:1474	On the other hand, the proliferation of sheep bone marrow cells on the proposed scaffolds was evaluated over 8 days.					
29717804	2	42	theme	polycaprolactone-based	361:382	arg1	scaffolds					384:392	polycaprolactone-based scaffolds	361:392	polycaprolactone-based scaffolds	361:392	Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering.					
29717804	1	43	from	matrices	181:188	arg1	additive					146:153	additive	146:153	additive	146:153	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	1	43	from	matrices	181:188	arg1	cellulose					98:106	Microcrystalline cellulose	81:106	Microcrystalline cellulose (MCC)	81:112	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	12	44	theme	engineering	1728:1738	arg1	applications					1740:1751	bone tissue engineering applications	1716:1751	bone tissue engineering applications	1716:1751	Therefore, these scaffolds (PCL:MCC 98:2) have suitable properties to be further evaluated for bone tissue engineering applications.					
29717804	12	45	theme	suitable	1668:1675	arg1	properties					1677:1686	suitable properties	1668:1686	suitable properties	1668:1686	Therefore, these scaffolds (PCL:MCC 98:2) have suitable properties to be further evaluated for bone tissue engineering applications.					
29717804	2	46	theme	bone	451:454	arg1	engineering					463:473	bone tissue engineering	451:473	bone tissue engineering	451:473	Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering.					
29717804	3	47	theme	additive	557:564	arg1	effect					543:548	the effect	539:548	the effect of the additive in the properties of the PCL matrix	539:600	Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix.					
29717804	1	48	theme	improved	246:253	arg1	properties					281:290	improved mechanical and biological properties	246:290	improved mechanical and biological properties	246:290	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	6	49	theme	3D	902:903	arg1	printer					905:911	a commercial 3D printer	889:911	a commercial 3D printer based on fused deposition modelling	889:947	3D printed scaffolds were manufactured with a commercial 3D printer based on fused deposition modelling.					
29717804	1	50	with	scaffolds	231:239	arg1	properties					281:290	improved mechanical and biological properties	246:290	improved mechanical and biological properties	246:290	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	12	51	theme	MCC	1653:1655	arg1	98:2					1657:1660	MCC 98:2	1653:1660	PCL:MCC 98:2	1649:1660	Therefore, these scaffolds (PCL:MCC 98:2) have suitable properties to be further evaluated for bone tissue engineering applications.					
29717804	7	52	theme	500	1079:1081	arg1	µm					1083:1084	450 and 500 µm	1071:1084	µm	1083:1084	The operation conditions have been established in order to obtain scaffolds with a 0/90° pattern with pore sizes between 450 and 500 µm and porosity values between 50 and 60%.					
29717804	4	53	theme	MCC	625:627	arg1	concentrations					607:620	The concentrations	603:620	The concentrations of MCC in the groups of samples	603:652	The concentrations of MCC in the groups of samples were 0, 2, 5, and 10% (w/w).					
29717804	4	53	theme	MCC	625:627	arg1	%					674:674	0, 2, 5, and 10%	659:674	0, 2, 5, and 10% (w/w)	659:680	The concentrations of MCC in the groups of samples were 0, 2, 5, and 10% (w/w).					
29717804	7	54	theme	450	1071:1073	arg1	µm					1083:1084	450 and 500 µm	1071:1084	µm	1083:1084	The operation conditions have been established in order to obtain scaffolds with a 0/90° pattern with pore sizes between 450 and 500 µm and porosity values between 50 and 60%.					
29717804	9	55	contain	containing	1332:1341	arg2	%					1345:1345	10%	1343:1345	10%	1343:1345	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	9	55	contain	containing	1332:1341	arg1	those					1326:1330	those	1326:1330	those	1326:1330	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	14	56	theme	Part	1804:1807	arg1	B					1809:1809	Part B	1804:1809	J Biomed Mater Res Part B: Appl Biomater, 2018.	1785:1831	J Biomed Mater Res Part B: Appl Biomater, 2018.					
29717804	8	57	theme	mechanical	1130:1139	arg1	properties					1141:1150	The mechanical properties	1126:1150	The mechanical properties of these structures	1126:1170	The mechanical properties of these structures were measured in the compression and flexural modes.					
29717804	3	58	from	effect	543:548	arg1	properties					573:582	the properties	569:582	the properties of the PCL matrix	569:600	Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix.					
29717804	3	59	theme	PCL	591:593	arg1	matrix					595:600	the PCL matrix	587:600	the PCL matrix	587:600	Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix.					
29717804	7	60	theme	0/90°	1033:1037	arg1	pattern					1039:1045	a 0/90° pattern	1031:1045	a 0/90° pattern with pore sizes between 450 and 500 µm and porosity values between 50 and 60%	1031:1123	The operation conditions have been established in order to obtain scaffolds with a 0/90° pattern with pore sizes between 450 and 500 µm and porosity values between 50 and 60%.					
29717804	8	61	theme	structures	1161:1170	arg1	properties					1141:1150	The mechanical properties	1126:1150	The mechanical properties of these structures	1126:1170	The mechanical properties of these structures were measured in the compression and flexural modes.					
29717804	6	62	theme	fused	922:926	arg1	modelling					939:947	fused deposition modelling	922:947	fused deposition modelling	922:947	3D printed scaffolds were manufactured with a commercial 3D printer based on fused deposition modelling.					
29717804	12	63	dep	PCL	1649:1651	arg1	98:2					1657:1660	MCC 98:2	1653:1660	PCL:MCC 98:2	1649:1660	Therefore, these scaffolds (PCL:MCC 98:2) have suitable properties to be further evaluated for bone tissue engineering applications.					
29717804	5	64	theme	thermogravimetric	722:738	arg1	analysis					740:747	a thermogravimetric analysis	720:747	a thermogravimetric analysis	720:747	These combinations were subjected to a thermogravimetric analysis in order to evaluate the influence of the additive in the thermal properties of the composites.					
29717804	1	65	theme	polycaprolactone	158:173	arg1	matrices					181:188	polycaprolactone (PCL) matrices	158:188	polycaprolactone (PCL) matrices	158:188	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	5	66	from	influence	774:782	arg1	properties					815:824	the thermal properties	803:824	the thermal properties of the composites	803:842	These combinations were subjected to a thermogravimetric analysis in order to evaluate the influence of the additive in the thermal properties of the composites.					
29717804	9	67	dep	flexural	1275:1282	arg1	higher					1268:1273	higher	1268:1273	higher	1268:1273	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	2	68	theme	structures	436:445	arg1	potential					417:425	the potential	413:425	the potential of these structures for bone tissue engineering	413:473	Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering.					
29717804	0	69	theme	printed	18:24	arg1	cellulose					60:68	Three-dimensional printed polycaprolactone-microcrystalline cellulose	0:68	Three-dimensional printed polycaprolactone-microcrystalline cellulose	0:68	Three-dimensional printed polycaprolactone-microcrystalline cellulose scaffolds.					
29717804	10	70	theme	other	1393:1397	arg1	hand					1399:1402	the other hand	1389:1402	the other hand	1389:1402	On the other hand, the proliferation of sheep bone marrow cells on the proposed scaffolds was evaluated over 8 days.					
29717804	5	71	theme	additive	791:798	arg1	influence					774:782	the influence	770:782	the influence of the additive in the thermal properties of the composites	770:842	These combinations were subjected to a thermogravimetric analysis in order to evaluate the influence of the additive in the thermal properties of the composites.					
29717804	8	72	dep	compression	1193:1203	arg1	the					1189:1191	the	1189:1191	the	1189:1191	The mechanical properties of these structures were measured in the compression and flexural modes.					
29717804	10	73	theme	sheep	1426:1430	arg1	cells					1444:1448	sheep bone marrow cells	1426:1448	sheep bone marrow cells	1426:1448	On the other hand, the proliferation of sheep bone marrow cells on the proposed scaffolds was evaluated over 8 days.					
29717804	11	74	theme	2	1613:1613	arg1	%					1614:1614	%	1614:1614	%	1614:1614	The results show that the proliferation is significantly better (p < 0.05) on the group of samples containing 2% MCC.					
29717804	9	75	theme	elastic	1300:1306	arg1	modulus					1308:1314	higher flexural and compression elastic modulus	1268:1314	modulus	1308:1314	The scaffolds containing 2 and 5% MCC have higher flexural and compression elastic modulus, although those containing 10% do not show this reinforcement effect.					
29717804	11	76	theme	samples	1594:1600	arg1	samples					1594:1600	samples	1594:1600	samples containing 2% MCC	1594:1618	The results show that the proliferation is significantly better (p < 0.05) on the group of samples containing 2% MCC.					
29717804	11	76	theme	samples	1594:1600	arg1	group					1585:1589	the group	1581:1589	the group of samples containing 2% MCC	1581:1618	The results show that the proliferation is significantly better (p < 0.05) on the group of samples containing 2% MCC.					
29717804	1	77	dep	three-dimensional	200:216	arg1	3D					219:220	3D	219:220	3D	219:220	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	14	78	dep	Res	1800:1802	arg1	2018					1827:1830	2018	1827:1830	2018	1827:1830	J Biomed Mater Res Part B: Appl Biomater, 2018.					
29717804	14	78	dep	Res	1800:1802	arg1	B					1809:1809	Part B	1804:1809	J Biomed Mater Res Part B: Appl Biomater, 2018.	1785:1831	J Biomed Mater Res Part B: Appl Biomater, 2018.					
29717804	14	78	dep	Res	1800:1802	arg1	Biomater					1817:1824	Biomater	1817:1824	Biomater	1817:1824	J Biomed Mater Res Part B: Appl Biomater, 2018.					
29717804	12	79	theme	tissue	1721:1726	arg1	applications					1740:1751	bone tissue engineering applications	1716:1751	bone tissue engineering applications	1716:1751	Therefore, these scaffolds (PCL:MCC 98:2) have suitable properties to be further evaluated for bone tissue engineering applications.					
29717804	2	80	theme	scaffolds	384:392	arg1	behavior					318:325	the mechanical behavior	303:325	the mechanical behavior	303:325	Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering.					
29717804	2	80	theme	scaffolds	384:392	arg1	performance					346:356	the biological performance	331:356	the biological performance	331:356	Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering.					
29717804	13	81	dep	©	1754:1754	arg1	Inc.					1780:1783	Inc.	1780:1783	Inc.	1780:1783	© 2018 Wiley Periodicals, Inc.					
29717804	10	82	theme	marrow	1437:1442	arg1	cells					1444:1448	sheep bone marrow cells	1426:1448	sheep bone marrow cells	1426:1448	On the other hand, the proliferation of sheep bone marrow cells on the proposed scaffolds was evaluated over 8 days.					
29717804	1	83	theme	Microcrystalline	81:96	arg1	additive					146:153	additive	146:153	additive	146:153	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	1	83	theme	Microcrystalline	81:96	arg1	MCC					109:111	MCC	109:111	MCC	109:111	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	1	83	theme	Microcrystalline	81:96	arg1	cellulose					98:106	Microcrystalline cellulose	81:106	Microcrystalline cellulose (MCC)	81:112	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
29717804	3	84	theme	samples	496:502	arg1	samples					496:502	samples	496:502	samples	496:502	Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix.					
29717804	3	84	theme	samples	496:502	arg1	groups					486:491	Different groups	476:491	Different groups of samples	476:502	Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix.					
29717804	4	85	theme	samples	646:652	arg1	groups					636:641	the groups	632:641	the groups of samples	632:652	The concentrations of MCC in the groups of samples were 0, 2, 5, and 10% (w/w).					
29717804	4	85	theme	samples	646:652	arg1	samples					646:652	samples	646:652	samples	646:652	The concentrations of MCC in the groups of samples were 0, 2, 5, and 10% (w/w).					
29717804	1	86	theme	mechanical	255:264	arg1	properties					281:290	improved mechanical and biological properties	246:290	improved mechanical and biological properties	246:290	Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties.					
31277502	10	0	from	caloric	1620:1626	arg1	weeks					1645:1649	the first two weeks	1631:1649	the first two weeks of lactation	1631:1662	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	2	1	theme	human	336:340	arg1	milk					342:345	human milk	336:345	human milk (HM)	336:350	As they have supplementary nutritional needs, very preterm infants may require fortification of human milk (HM).					
31277502	2	1	theme	human	336:340	arg1	HM					348:349	HM	348:349	HM	348:349	As they have supplementary nutritional needs, very preterm infants may require fortification of human milk (HM).					
31277502	7	2	theme	intra-subject	1271:1283	arg1	variations					1285:1294	large inter- and intra-subject variations	1254:1294	large inter- and intra-subject variations in both groups	1254:1309	RESULTS Analysis of 500 HM samples revealed large inter- and intra-subject variations in both groups.					
31277502	4	3	theme	other	756:760	arg1	factors					772:778	other potential factors	756:778	other potential factors of variations	756:792	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	8	4	theme	time	1429:1432	arg1	point					1434:1438	any postnatal time point	1415:1438	any postnatal time point	1415:1438	Proteins decreased from birth to four months in the PT and the T HM without significant differences at any postnatal time point, while it was lower around term equivalent age in PT HM.					
31277502	7	5	theme	inter-	1260:1265	arg1	variations					1285:1294	large inter- and intra-subject variations	1254:1294	large inter- and intra-subject variations in both groups	1254:1309	RESULTS Analysis of 500 HM samples revealed large inter- and intra-subject variations in both groups.					
31277502	4	6	theme	postmenstrual	717:729	arg1	ages					731:734	similar postnatal and postmenstrual ages	695:734	ages	731:734	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	4	7	theme	age	615:617	arg1	weeks					594:598	28 0/7 to 32 6/7 weeks	577:598	28 0/7 to 32 6/7 weeks of gestational age	577:617	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	4	7	theme	age	615:617	arg1	preterm					563:569	preterm	563:569	preterm	563:569	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	8	8	theme	PT	1490:1491	arg1	HM					1493:1494	PT HM	1490:1494	PT HM	1490:1494	Proteins decreased from birth to four months in the PT and the T HM without significant differences at any postnatal time point, while it was lower around term equivalent age in PT HM.					
31277502	4	9	dep	time	681:684	arg1	both					687:690	both	687:690	both	687:690	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	14	10	theme	similar	2157:2163	arg1	ages					2179:2182	similar postmenstrual ages	2157:2182	similar postmenstrual ages	2157:2182	However, numerous differences exist at similar postmenstrual ages.					
31277502	2	11	theme	preterm	291:297	arg1	infants					299:305	very preterm infants	286:305	very preterm infants	286:305	As they have supplementary nutritional needs, very preterm infants may require fortification of human milk (HM).					
31277502	1	12	theme	nutrients	165:173	arg1	milk					135:138	BACKGROUND Mother's own milk	111:138	BACKGROUND Mother's own milk	111:138	BACKGROUND Mother's own milk is the optimal source of nutrients and provides numerous health advantages for mothers and infants.					
31277502	1	12	theme	nutrients	165:173	arg1	source					155:160	the optimal source	143:160	the optimal source of nutrients	143:173	BACKGROUND Mother's own milk is the optimal source of nutrients and provides numerous health advantages for mothers and infants.					
31277502	4	13	theme	6/7	590:592	arg1	weeks					594:598	28 0/7 to 32 6/7 weeks	577:598	28 0/7 to 32 6/7 weeks of gestational age	577:617	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	4	13	theme	6/7	590:592	arg1	preterm					563:569	preterm	563:569	preterm	563:569	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	10	14	theme	higher	1689:1694	arg1	fat					1696:1698	higher fat	1689:1698	higher fat	1689:1698	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	13	15	theme	PT	2025:2026	arg1	compositions					2005:2016	compositions	2005:2016	compositions of the PT	2005:2026	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	1	16	theme	BACKGROUND	111:120	arg1	Mother					122:127	BACKGROUND Mother's	111:129	BACKGROUND Mother's own milk	111:138	BACKGROUND Mother's own milk is the optimal source of nutrients and provides numerous health advantages for mothers and infants.					
31277502	16	17	theme	PT	2446:2447	arg1	infants					2449:2455	PT infants	2446:2455	PT infants	2446:2455	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	8	18	from	HM	1377:1378	arg1	PT					1364:1365	the PT	1360:1365	the PT	1360:1365	Proteins decreased from birth to four months in the PT and the T HM without significant differences at any postnatal time point, while it was lower around term equivalent age in PT HM.					
31277502	5	19	from	months	945:950	arg1	time					986:989	time	986:989	time during two months for the T HM	986:1020	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	1	20	theme	health	197:202	arg1	advantages					204:213	numerous health advantages	188:213	numerous health advantages for mothers and infants	188:237	BACKGROUND Mother's own milk is the optimal source of nutrients and provides numerous health advantages for mothers and infants.					
31277502	7	21	from	variations	1285:1294	arg1	groups					1304:1309	both groups	1299:1309	both groups	1299:1309	RESULTS Analysis of 500 HM samples revealed large inter- and intra-subject variations in both groups.					
31277502	4	22	theme	variations	783:792	arg1	factors					772:778	other potential factors	756:778	other potential factors of variations	756:792	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	10	23	theme	energy	1711:1716	arg1	content					1718:1724	higher energy content	1704:1724	higher energy content	1704:1724	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	6	24	theme	mid-infrared	1111:1122	arg1	analyzer					1129:1136	a mid-infrared milk analyzer	1109:1136	a mid-infrared milk analyzer	1109:1136	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	10	25	theme	PT	1559:1560	arg1	caloric					1620:1626	caloric	1620:1626	caloric	1620:1626	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	10	25	theme	PT	1559:1560	arg1	HM					1562:1563	The PT HM	1555:1563	The PT HM	1555:1563	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	15	26	theme	Male	2185:2188	arg1	gender					2190:2195	Male gender	2185:2195	Male gender	2185:2195	Male gender seems to be associated with a higher content in fat, especially in the PT HM.					
31277502	5	27	theme	METHODS	795:801	arg1	samples					808:814	METHODS Milk samples	795:814	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants	795:880	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	9	28	theme	Lactose	1497:1503	arg1	content					1505:1511	Lactose content	1497:1511	Lactose content	1497:1511	Lactose content remained stable and comparable over time.					
31277502	6	29	dep	composition	1037:1047	arg1	lactose					1069:1075	lactose	1069:1075	lactose	1069:1075	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	6	29	dep	composition	1037:1047	arg1	proteins					1050:1057	proteins	1050:1057	proteins	1050:1057	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	6	29	dep	composition	1037:1047	arg1	fat					1060:1062	fat	1060:1062	fat	1060:1062	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	4	30	dep	0/7	649:651	arg1	to					653:654	to	653:654	to	653:654	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	5	31	from	points	918:923	arg1	time					928:931	time	928:931	time	928:931	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	10	32	theme	more	1589:1592	arg1	fat					1594:1596	significantly more fat	1575:1596	significantly more fat	1575:1596	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	13	33	theme	CONCLUSION	1950:1959	arg1	measurements					1974:1985	CONCLUSION Longitudinal measurements	1950:1985	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM	1950:2039	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	13	34	theme	macronutrients	1990:2003	arg1	measurements					1974:1985	CONCLUSION Longitudinal measurements	1950:1985	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM	1950:2039	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	15	35	theme	higher	2227:2232	arg1	content					2234:2240	a higher content	2225:2240	a higher content	2225:2240	Male gender seems to be associated with a higher content in fat, especially in the PT HM.					
31277502	5	36	theme	PT	839:840	arg1	infants					842:848	the PT infants	835:848	the PT infants	835:848	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	4	37	theme	GA	672:673	arg1	weeks					663:667	37 0/7 to 41 6/7 weeks	646:667	37 0/7 to 41 6/7 weeks of GA	646:673	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	0	38	theme	Cohort	97:102	arg1	Study					104:108	A Prospective Cohort Study	83:108	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.	0:109	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
31277502	3	39	theme	Addressing	353:362	arg1	composition					367:377	Addressing HM composition	353:377	Addressing HM composition	353:377	Addressing HM composition and variations is essential to optimize HM fortification strategies for these vulnerable infants.					
31277502	0	40	theme	Human	71:75	arg1	Milk					77:80	Term Human Milk	66:80	Term Human Milk	66:80	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
31277502	13	41	from	differences	2059:2069	arg1	population					2106:2115	our population	2102:2115	our population	2102:2115	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	0	42	theme	Longitudinal	0:11	arg1	Analysis					13:20	Longitudinal Analysis	0:20	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.	0:109	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
31277502	2	43	contain	have	248:251	arg2	nutritional					267:277	nutritional	267:277	nutritional	267:277	As they have supplementary nutritional needs, very preterm infants may require fortification of human milk (HM).					
31277502	2	43	contain	have	248:251	arg1	they					243:246	they	243:246	they	243:246	As they have supplementary nutritional needs, very preterm infants may require fortification of human milk (HM).					
31277502	4	44	dep	infants	637:643	arg1	weeks					663:667	37 0/7 to 41 6/7 weeks	646:667	37 0/7 to 41 6/7 weeks of GA	646:673	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	10	45	contain	contained	1565:1573	arg2	fat					1594:1596	significantly more fat	1575:1596	significantly more fat	1575:1596	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	10	45	contain	contained	1565:1573	arg1	caloric					1620:1626	caloric	1620:1626	caloric	1620:1626	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	10	45	contain	contained	1565:1573	arg1	HM					1562:1563	The PT HM	1555:1563	The PT HM	1555:1563	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	4	46	theme	41	656:657	arg1	0/7					649:651	0/7	649:651	0/7	649:651	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	13	47	theme	postnatal	2082:2090	arg1	stages					2092:2097	similar postnatal stages	2074:2097	similar postnatal stages	2074:2097	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	13	48	from	stages	2092:2097	arg1	differences					2059:2069	only small differences	2048:2069	only small differences at similar postnatal stages in our population	2048:2115	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	3	49	theme	fortification	422:434	arg1	strategies					436:445	HM fortification strategies	419:445	HM fortification strategies for these vulnerable infants	419:474	Addressing HM composition and variations is essential to optimize HM fortification strategies for these vulnerable infants.					
31277502	0	50	theme	Composition	39:49	arg1	Analysis					13:20	Longitudinal Analysis	0:20	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.	0:109	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
31277502	5	51	from	points	976:981	arg1	time					986:989	time	986:989	time during two months for the T HM	986:1020	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	4	52	dep	weeks	594:598	arg1	GA					620:621	GA	620:621	GA	620:621	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	4	53	theme	macronutrient	505:517	arg1	composition					519:529	macronutrient composition	505:529	macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA)	505:674	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	0	54	theme	Term	66:69	arg1	Milk					77:80	Term Human Milk	66:80	Term Human Milk	66:80	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
31277502	12	55	theme	PT	1893:1894	arg1	group					1896:1900	the PT group	1889:1900	the PT group	1889:1900	The gender association was stronger in the PT group, and it remained significant after adjustments.					
31277502	14	56	theme	postmenstrual	2165:2177	arg1	ages					2179:2182	similar postmenstrual ages	2157:2182	similar postmenstrual ages	2157:2182	However, numerous differences exist at similar postmenstrual ages.					
31277502	6	57	theme	protein	1193:1199	arg1	content					1201:1207	total protein content	1187:1207	total protein content	1187:1207	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	4	58	dep	32	587:588	arg1	to					584:585	to	584:585	to	584:585	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	10	59	theme	first	1635:1639	arg1	weeks					1645:1649	the first two weeks	1631:1649	the first two weeks of lactation	1631:1662	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	12	60	theme	gender	1854:1859	arg1	association					1861:1871	The gender association	1850:1871	The gender association	1850:1871	The gender association was stronger in the PT group, and it remained significant after adjustments.					
31277502	12	60	theme	gender	1854:1859	arg1	stronger					1877:1884	stronger	1877:1884	stronger	1877:1884	The gender association was stronger in the PT group, and it remained significant after adjustments.					
31277502	5	61	theme	infants	874:880	arg1	mothers					824:830	27 mothers	821:830	27 mothers of the PT infants	821:848	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	5	61	theme	infants	874:880	arg1	mothers					857:863	34 mothers	854:863	34 mothers of the T infants	854:880	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	3	62	theme	vulnerable	457:466	arg1	infants					468:474	these vulnerable infants	451:474	these vulnerable infants	451:474	Addressing HM composition and variations is essential to optimize HM fortification strategies for these vulnerable infants.					
31277502	16	63	theme	HM	2363:2364	arg1	variations					2349:2358	variations	2349:2358	variations	2349:2358	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	16	63	theme	HM	2363:2364	arg1	composition					2333:2343	macronutrient composition	2319:2343	macronutrient composition	2319:2343	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	13	64	theme	HM	2038:2039	arg1	measurements					1974:1985	CONCLUSION Longitudinal measurements	1950:1985	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM	1950:2039	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	13	65	theme	similar	2074:2080	arg1	stages					2092:2097	similar postnatal stages	2074:2097	similar postnatal stages	2074:2097	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	8	66	theme	significant	1388:1398	arg1	differences					1400:1410	significant differences	1388:1410	significant differences at any postnatal time point	1388:1438	Proteins decreased from birth to four months in the PT and the T HM without significant differences at any postnatal time point, while it was lower around term equivalent age in PT HM.					
31277502	10	67	theme	lactation	1654:1662	arg1	weeks					1645:1649	the first two weeks	1631:1649	the first two weeks of lactation	1631:1662	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	16	68	from	information	2304:2314	arg1	variations					2349:2358	variations	2349:2358	variations	2349:2358	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	16	68	from	information	2304:2314	arg1	composition					2333:2343	macronutrient composition	2319:2343	macronutrient composition	2319:2343	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	2	69	theme	milk	342:345	arg1	fortification					319:331	fortification	319:331	fortification of human milk (HM)	319:350	As they have supplementary nutritional needs, very preterm infants may require fortification of human milk (HM).					
31277502	8	70	from	months	1350:1355	arg1	PT					1364:1365	the PT	1360:1365	the PT	1360:1365	Proteins decreased from birth to four months in the PT and the T HM without significant differences at any postnatal time point, while it was lower around term equivalent age in PT HM.					
31277502	7	71	theme	large	1254:1258	arg1	variations					1285:1294	large inter- and intra-subject variations	1254:1294	large inter- and intra-subject variations in both groups	1254:1309	RESULTS Analysis of 500 HM samples revealed large inter- and intra-subject variations in both groups.					
31277502	7	72	theme	HM	1234:1235	arg1	samples					1237:1243	500 HM samples	1230:1243	500 HM samples	1230:1243	RESULTS Analysis of 500 HM samples revealed large inter- and intra-subject variations in both groups.					
31277502	8	73	theme	postnatal	1419:1427	arg1	point					1434:1438	any postnatal time point	1415:1438	any postnatal time point	1415:1438	Proteins decreased from birth to four months in the PT and the T HM without significant differences at any postnatal time point, while it was lower around term equivalent age in PT HM.					
31277502	10	74	theme	T	1675:1675	arg1	HM					1677:1678	the T HM	1671:1678	the T HM	1671:1678	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	8	75	theme	equivalent	1472:1481	arg1	age					1483:1485	term equivalent age	1467:1485	term equivalent age	1467:1485	Proteins decreased from birth to four months in the PT and the T HM without significant differences at any postnatal time point, while it was lower around term equivalent age in PT HM.					
31277502	1	76	theme	optimal	147:153	arg1	milk					135:138	BACKGROUND Mother's own milk	111:138	BACKGROUND Mother's own milk	111:138	BACKGROUND Mother's own milk is the optimal source of nutrients and provides numerous health advantages for mothers and infants.					
31277502	1	76	theme	optimal	147:153	arg1	source					155:160	the optimal source	143:160	the optimal source of nutrients	143:173	BACKGROUND Mother's own milk is the optimal source of nutrients and provides numerous health advantages for mothers and infants.					
31277502	7	77	theme	samples	1237:1243	arg1	Analysis					1218:1225	RESULTS Analysis	1210:1225	RESULTS Analysis of 500 HM samples	1210:1243	RESULTS Analysis of 500 HM samples revealed large inter- and intra-subject variations in both groups.					
31277502	4	78	theme	gestational	603:613	arg1	age					615:617	gestational age	603:617	gestational age	603:617	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	13	79	theme	T	2036:2036	arg1	HM					2038:2039	the T HM	2032:2039	the T HM	2032:2039	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	5	80	from	mothers	857:863	arg1	samples					808:814	METHODS Milk samples	795:814	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants	795:880	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	10	81	from	weeks	1645:1649	arg1	caloric					1620:1626	caloric	1620:1626	caloric	1620:1626	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	10	81	from	weeks	1645:1649	arg1	HM					1562:1563	The PT HM	1555:1563	The PT HM	1555:1563	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	11	82	theme	energy	1834:1839	arg1	content					1841:1847	energy content	1834:1847	energy content	1834:1847	In both groups, male gender was associated with more fat and energy content.					
31277502	7	83	theme	RESULTS	1210:1216	arg1	Analysis					1218:1225	RESULTS Analysis	1210:1225	RESULTS Analysis of 500 HM samples	1210:1243	RESULTS Analysis of 500 HM samples revealed large inter- and intra-subject variations in both groups.					
31277502	10	84	dep	eight	1759:1763	arg1	to					1756:1757	to	1756:1757	to	1756:1757	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	2	85	dep	nutritional	267:277	arg1	needs					279:283	needs	279:283	needs	279:283	As they have supplementary nutritional needs, very preterm infants may require fortification of human milk (HM).					
31277502	1	86	theme	numerous	188:195	arg1	advantages					204:213	numerous health advantages	188:213	numerous health advantages for mothers and infants	188:237	BACKGROUND Mother's own milk is the optimal source of nutrients and provides numerous health advantages for mothers and infants.					
31277502	16	87	theme	macronutrient	2319:2331	arg1	composition					2333:2343	macronutrient composition	2319:2343	macronutrient composition	2319:2343	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	14	88	theme	numerous	2127:2134	arg1	differences					2136:2146	numerous differences	2127:2146	numerous differences	2127:2146	However, numerous differences exist at similar postmenstrual ages.					
31277502	6	89	theme	milk	1124:1127	arg1	analyzer					1129:1136	a mid-infrared milk analyzer	1109:1136	a mid-infrared milk analyzer	1109:1136	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	4	90	theme	potential	762:770	arg1	factors					772:778	other potential factors	756:778	other potential factors of variations	756:792	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	16	91	theme	infants	2449:2455	arg1	nutrition					2422:2430	nutrition	2422:2430	nutrition	2422:2430	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	16	91	theme	infants	2449:2455	arg1	growth					2436:2441	growth	2436:2441	growth	2436:2441	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	4	92	theme	T	634:634	arg1	infants					637:643	term (T) infants	628:643	term (T) infants (37 0/7 to 41 6/7 weeks of GA)	628:674	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	10	93	theme	higher	1704:1709	arg1	content					1718:1724	higher energy content	1704:1724	higher energy content	1704:1724	The PT HM contained significantly more fat and tended to be more caloric in the first two weeks of lactation, while the T HM revealed higher fat and higher energy content later during lactation (three to eight weeks).					
31277502	13	94	theme	small	2053:2057	arg1	differences					2059:2069	only small differences	2048:2069	only small differences at similar postnatal stages in our population	2048:2115	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	5	95	theme	Milk	803:806	arg1	samples					808:814	METHODS Milk samples	795:814	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants	795:880	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	11	96	theme	male	1789:1792	arg1	gender					1794:1799	male gender	1789:1799	male gender	1789:1799	In both groups, male gender was associated with more fat and energy content.					
31277502	6	97	theme	Macronutrient	1023:1035	arg1	composition					1037:1047	Macronutrient composition	1023:1047	Macronutrient composition (proteins, fat, and lactose)	1023:1076	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	4	98	theme	infants	637:643	arg1	composition					519:529	macronutrient composition	505:529	macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA)	505:674	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	6	99	theme	total	1187:1191	arg1	content					1201:1207	total protein content	1187:1207	total protein content	1187:1207	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	1	100	theme	own	131:133	arg1	milk					135:138	BACKGROUND Mother's own milk	111:138	BACKGROUND Mother's own milk	111:138	BACKGROUND Mother's own milk is the optimal source of nutrients and provides numerous health advantages for mothers and infants.					
31277502	1	100	theme	own	131:133	arg1	source					155:160	the optimal source	143:160	the optimal source of nutrients	143:173	BACKGROUND Mother's own milk is the optimal source of nutrients and provides numerous health advantages for mothers and infants.					
31277502	15	101	from	content	2234:2240	arg1	HM					2271:2272	the PT HM	2264:2272	the PT HM	2264:2272	Male gender seems to be associated with a higher content in fat, especially in the PT HM.					
31277502	15	101	from	content	2234:2240	arg1	fat					2245:2247	fat	2245:2247	fat	2245:2247	Male gender seems to be associated with a higher content in fat, especially in the PT HM.					
31277502	13	102	theme	Longitudinal	1961:1972	arg1	measurements					1974:1985	CONCLUSION Longitudinal measurements	1950:1985	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM	1950:2039	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	0	103	from	Analysis	13:20	arg1	Milk					77:80	Term Human Milk	66:80	Term Human Milk	66:80	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
31277502	0	103	from	Analysis	13:20	arg1	Preterm					54:60	Preterm	54:60	Preterm	54:60	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
31277502	4	104	theme	term	628:631	arg1	infants					637:643	term (T) infants	628:643	term (T) infants (37 0/7 to 41 6/7 weeks of GA)	628:674	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	0	105	theme	Prospective	85:95	arg1	Study					104:108	A Prospective Cohort Study	83:108	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.	0:109	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
31277502	6	106	theme	acid	1166:1169	arg1	assay					1177:1181	bicinchoninic acid (BCA) assay	1152:1181	bicinchoninic acid (BCA) assay for total protein content	1152:1207	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	5	107	theme	infants	842:848	arg1	mothers					824:830	27 mothers	821:830	27 mothers of the PT infants	821:848	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	5	107	theme	infants	842:848	arg1	mothers					857:863	34 mothers	854:863	34 mothers of the T infants	854:880	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	8	108	from	point	1434:1438	arg1	differences					1400:1410	significant differences	1388:1410	significant differences at any postnatal time point	1388:1438	Proteins decreased from birth to four months in the PT and the T HM without significant differences at any postnatal time point, while it was lower around term equivalent age in PT HM.					
31277502	3	109	theme	HM	419:420	arg1	strategies					436:445	HM fortification strategies	419:445	HM fortification strategies for these vulnerable infants	419:474	Addressing HM composition and variations is essential to optimize HM fortification strategies for these vulnerable infants.					
31277502	5	110	theme	PT	960:961	arg1	HM					963:964	the PT HM	956:964	the PT HM	956:964	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	3	111	theme	HM	364:365	arg1	composition					367:377	Addressing HM composition	353:377	Addressing HM composition	353:377	Addressing HM composition and variations is essential to optimize HM fortification strategies for these vulnerable infants.					
31277502	4	112	theme	mothers	540:546	arg1	composition					519:529	macronutrient composition	505:529	macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA)	505:674	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	4	113	theme	37	646:647	arg1	0/7					649:651	0/7	649:651	0/7	649:651	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	0	114	theme	Macronutrient	25:37	arg1	Composition					39:49	Macronutrient Composition	25:49	Macronutrient Composition	25:49	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
31277502	16	115	theme	nutrition	2422:2430	arg1	optimization					2406:2417	the optimization	2402:2417	the optimization of nutrition and growth of PT infants	2402:2455	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	5	116	theme	T	1017:1017	arg1	HM					1019:1020	the T HM	1013:1020	the T HM	1013:1020	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	5	117	theme	T	872:872	arg1	infants					874:880	the T infants	868:880	the T infants	868:880	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	16	118	theme	growth	2436:2441	arg1	optimization					2406:2417	the optimization	2402:2417	the optimization of nutrition and growth of PT infants	2402:2455	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	6	119	theme	bicinchoninic	1152:1164	arg1	BCA					1172:1174	BCA	1172:1174	BCA	1172:1174	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	6	119	theme	bicinchoninic	1152:1164	arg1	acid					1166:1169	bicinchoninic acid	1152:1169	bicinchoninic acid (BCA) assay for total protein content	1152:1207	Macronutrient composition (proteins, fat, and lactose) and energy were measured using a mid-infrared milk analyzer, corrected by bicinchoninic acid (BCA) assay for total protein content.					
31277502	4	120	from	composition	519:529	arg1	HM					534:535	HM	534:535	HM	534:535	AIMS To analyze and compare macronutrient composition in HM of mothers lactating very preterm (PT) (28 0/7 to 32 6/7 weeks of gestational age, GA) and term (T) infants (37 0/7 to 41 6/7 weeks of GA) over time, both at similar postnatal and postmenstrual ages, and to investigate other potential factors of variations.					
31277502	16	121	theme	original	2295:2302	arg1	information					2304:2314	original information	2295:2314	original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants	2295:2455	This study provides original information on macronutrient composition and variations of HM, which is important to consider for the optimization of nutrition and growth of PT infants.					
31277502	8	122	theme	T	1375:1375	arg1	HM					1377:1378	the T HM	1371:1378	the T HM	1371:1378	Proteins decreased from birth to four months in the PT and the T HM without significant differences at any postnatal time point, while it was lower around term equivalent age in PT HM.					
31277502	5	123	from	mothers	824:830	arg1	samples					808:814	METHODS Milk samples	795:814	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants	795:880	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	5	124	theme	34	854:855	arg1	mothers					857:863	34 mothers	854:863	34 mothers of the T infants	854:880	METHODS Milk samples from 27 mothers of the PT infants and 34 mothers of the T infants were collected longitudinally at 12 points in time during four months for the PT HM and eight points in time during two months for the T HM.					
31277502	13	125	dep	macronutrients	1990:2003	arg1	compositions					2005:2016	compositions	2005:2016	compositions of the PT	2005:2026	CONCLUSION Longitudinal measurements of macronutrients compositions of the PT and the T HM showed only small differences at similar postnatal stages in our population.					
31277502	15	126	theme	PT	2268:2269	arg1	HM					2271:2272	the PT HM	2264:2272	the PT HM	2264:2272	Male gender seems to be associated with a higher content in fat, especially in the PT HM.					
31277502	0	127	dep	Analysis	13:20	arg1	Study					104:108	A Prospective Cohort Study	83:108	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.	0:109	Longitudinal Analysis of Macronutrient Composition in Preterm and Term Human Milk: A Prospective Cohort Study.					
29758540	4	0	theme	anticoagulant	1064:1076	arg1	activity					1078:1085	good anticoagulant activity	1059:1085	good anticoagulant activity	1059:1085	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	1	1	theme	implant-related	207:221	arg1	infections					223:232	avoiding implant-related infections	198:232	avoiding implant-related infections	198:232	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid.					
29758540	0	2	theme	electrospun	113:123	arg1	membranes					125:133	electrospun membranes	113:133	electrospun membranes	113:133	Integrated antibacterial and antifouling surfaces via cross-linking chitosan-g-eugenol/zwitterionic copolymer on electrospun membranes.					
29758540	4	3	theme	good	1059:1062	arg1	activity					1078:1085	good anticoagulant activity	1059:1085	good anticoagulant activity	1059:1085	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	6	4	theme	integrated	1284:1293	arg1	properties					1325:1334	integrated antibacterial and antifouling properties	1284:1334	integrated antibacterial and antifouling properties	1284:1334	Therefore, the membranes with integrated antibacterial and antifouling properties could be potentially applied in promising indwelling devices.					
29758540	6	5	theme	antibacterial	1295:1307	arg1	properties					1325:1334	integrated antibacterial and antifouling properties	1284:1334	integrated antibacterial and antifouling properties	1284:1334	Therefore, the membranes with integrated antibacterial and antifouling properties could be potentially applied in promising indwelling devices.					
29758540	3	6	theme	antibacterial	677:689	arg1	activity					691:698	efficient antibacterial activity	667:698	efficient antibacterial activity	667:698	Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively.					
29758540	6	7	theme	indwelling	1378:1387	arg1	devices					1389:1395	promising indwelling devices	1368:1395	promising indwelling devices	1368:1395	Therefore, the membranes with integrated antibacterial and antifouling properties could be potentially applied in promising indwelling devices.					
29758540	6	8	theme	promising	1368:1376	arg1	devices					1389:1395	promising indwelling devices	1368:1395	promising indwelling devices	1368:1395	Therefore, the membranes with integrated antibacterial and antifouling properties could be potentially applied in promising indwelling devices.					
29758540	4	9	theme	bacterial	939:947	arg1	attachment					949:958	bacterial attachment	939:958	bacterial attachment	939:958	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	2	10	theme	antibacterial	332:344	arg1	membrane					362:369	an antibacterial and antifouling membrane	329:369	an antibacterial and antifouling membrane	329:369	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	4	11	from	investigations	827:840	arg1	activity					857:864	antifouling activity	845:864	antifouling activity	845:864	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	4	11	from	investigations	827:840	arg1	hemocompatibility					870:886	hemocompatibility	870:886	hemocompatibility	870:886	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	3	12	theme	Antibacterial	601:613	arg1	assays					615:620	Antibacterial assays	601:620	Antibacterial assays	601:620	Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively.					
29758540	5	13	theme	cell	1106:1109	arg1	assays					1119:1124	cell culture assays	1106:1124	cell culture assays	1106:1124	Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability.					
29758540	4	14	theme	hemolytic	1036:1044	arg1	activity					1046:1053	lower hemolytic activity	1030:1053	lower hemolytic activity	1030:1053	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	4	15	theme	non-specific	961:972	arg1	adsorption					982:991	non-specific protein adsorption	961:991	non-specific protein adsorption	961:991	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	4	16	theme	lower	1030:1034	arg1	activity					1046:1053	lower hemolytic activity	1030:1053	lower hemolytic activity	1030:1053	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	3	17	theme	efficient	667:675	arg1	activity					691:698	efficient antibacterial activity	667:698	efficient antibacterial activity	667:698	Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively.					
29758540	2	18	theme	methylacrylate-co-2-aminoethyl	471:500	arg1	methacrylate					502:513	sulfobetaine methylacrylate-co-2-aminoethyl methacrylate	458:513	sulfobetaine methylacrylate-co-2-aminoethyl methacrylate	458:513	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	2	18	theme	methylacrylate-co-2-aminoethyl	471:500	arg1	poly					453:456	the zwitterionic copolymer poly	426:456	the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate)	426:514	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	0	19	theme	antibacterial	11:23	arg1	surfaces					41:48	antibacterial and antifouling surfaces	11:48	antibacterial and antifouling surfaces	11:48	Integrated antibacterial and antifouling surfaces via cross-linking chitosan-g-eugenol/zwitterionic copolymer on electrospun membranes.					
29758540	4	20	theme	significant	912:922	arg1	resistances					924:934	significant resistances	912:934	significant resistances	912:934	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	2	21	theme	sulfobetaine	458:469	arg1	methacrylate					502:513	sulfobetaine methylacrylate-co-2-aminoethyl methacrylate	458:513	sulfobetaine methylacrylate-co-2-aminoethyl methacrylate	458:513	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	2	21	theme	sulfobetaine	458:469	arg1	poly					453:456	the zwitterionic copolymer poly	426:456	the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate)	426:514	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	1	22	theme	Integrated	136:145	arg1	surfaces					177:184	Integrated antibacterial and antifouling surfaces	136:184	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections	136:232	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid.					
29758540	2	23	theme	urethane	549:556	arg1	substrate					558:566	the electrospun polycarbonate urethane substrate	519:566	the electrospun polycarbonate urethane substrate using genipin as a cross-linker	519:598	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	1	24	from	surfaces	177:184	arg1	favor					189:193	favor	189:193	favor	189:193	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid.					
29758540	1	25	theme	antibacterial	147:159	arg1	surfaces					177:184	Integrated antibacterial and antifouling surfaces	136:184	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections	136:232	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid.					
29758540	2	26	theme	electrospun	523:533	arg1	substrate					558:566	the electrospun polycarbonate urethane substrate	519:566	the electrospun polycarbonate urethane substrate using genipin as a cross-linker	519:598	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	2	27	theme	polycarbonate	535:547	arg1	substrate					558:566	the electrospun polycarbonate urethane substrate	519:566	the electrospun polycarbonate urethane substrate using genipin as a cross-linker	519:598	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	1	28	with	contact	286:292	arg1	fluid					308:312	the body fluid	299:312	the body fluid	299:312	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid.					
29758540	2	29	theme	antifouling	350:360	arg1	membrane					362:369	an antibacterial and antifouling membrane	329:369	an antibacterial and antifouling membrane	329:369	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	3	30	theme	prepared	644:651	arg1	membranes					653:661	the prepared membranes	640:661	the prepared membranes	640:661	Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively.					
29758540	3	31	theme	growth	733:738	arg1	rates					751:755	growth inhibition rates	733:755	growth inhibition rates	733:755	Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively.					
29758540	4	32	theme	antifouling	845:855	arg1	activity					857:864	antifouling activity	845:864	antifouling activity	845:864	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	0	33	theme	antifouling	29:39	arg1	surfaces					41:48	antibacterial and antifouling surfaces	11:48	antibacterial and antifouling surfaces	11:48	Integrated antibacterial and antifouling surfaces via cross-linking chitosan-g-eugenol/zwitterionic copolymer on electrospun membranes.					
29758540	0	34	theme	chitosan-g-eugenol/zwitterionic	68:98	arg1	copolymer					100:108	chitosan-g-eugenol/zwitterionic copolymer	68:108	chitosan-g-eugenol/zwitterionic copolymer	68:108	Integrated antibacterial and antifouling surfaces via cross-linking chitosan-g-eugenol/zwitterionic copolymer on electrospun membranes.					
29758540	3	35	contain	had	663:665	arg2	activity					691:698	efficient antibacterial activity	667:698	efficient antibacterial activity	667:698	Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively.					
29758540	3	35	contain	had	663:665	arg1	membranes					653:661	the prepared membranes	640:661	the prepared membranes	640:661	Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively.					
29758540	6	36	with	membranes	1269:1277	arg1	properties					1325:1334	integrated antibacterial and antifouling properties	1284:1334	integrated antibacterial and antifouling properties	1284:1334	Therefore, the membranes with integrated antibacterial and antifouling properties could be potentially applied in promising indwelling devices.					
29758540	5	37	theme	relative	1218:1225	arg1	viability					1243:1251	relative L929 fibroblast viability	1218:1251	relative L929 fibroblast viability	1218:1251	Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability.					
29758540	6	38	theme	antifouling	1313:1323	arg1	properties					1325:1334	integrated antibacterial and antifouling properties	1284:1334	integrated antibacterial and antifouling properties	1284:1334	Therefore, the membranes with integrated antibacterial and antifouling properties could be potentially applied in promising indwelling devices.					
29758540	1	39	theme	antifouling	165:175	arg1	surfaces					177:184	Integrated antibacterial and antifouling surfaces	136:184	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections	136:232	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid.					
29758540	5	40	theme	fibroblast	1232:1241	arg1	viability					1243:1251	relative L929 fibroblast viability	1218:1251	relative L929 fibroblast viability	1218:1251	Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability.					
29758540	5	41	theme	L929	1227:1230	arg1	viability					1243:1251	relative L929 fibroblast viability	1218:1251	relative L929 fibroblast viability	1218:1251	Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability.					
29758540	5	42	theme	culture	1111:1117	arg1	assays					1119:1124	cell culture assays	1106:1124	cell culture assays	1106:1124	Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability.					
29758540	5	43	theme	viability	1243:1251	arg1	viability					1243:1251	relative L929 fibroblast viability	1218:1251	relative L929 fibroblast viability	1218:1251	Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability.					
29758540	5	43	theme	viability	1243:1251	arg1	%					1213:1213	more than 80%	1201:1213	more than 80% of relative L929 fibroblast viability	1201:1251	Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability.					
29758540	1	44	dep	they	281:284	arg1	contact					286:292	contact	286:292	contact	286:292	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid.					
29758540	3	45	theme	inhibition	740:749	arg1	rates					751:755	growth inhibition rates	733:755	growth inhibition rates	733:755	Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively.					
29758540	3	46	dep	%	715:715	arg1	rates					751:755	growth inhibition rates	733:755	growth inhibition rates	733:755	Antibacterial assays demonstrated that the prepared membranes had efficient antibacterial activity with 92.8 ± 2.5% and 95.2 ± 1.3% growth inhibition rates against Escherichia coli and Staphylococcus aureus, respectively.					
29758540	5	47	theme	prepared	1145:1152	arg1	membranes					1154:1162	the prepared membranes	1141:1162	the prepared membranes	1141:1162	Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability.					
29758540	4	48	theme	protein	974:980	arg1	adsorption					982:991	non-specific protein adsorption	961:991	non-specific protein adsorption	961:991	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	1	49	theme	avoiding	198:205	arg1	infections					223:232	avoiding implant-related infections	198:232	avoiding implant-related infections	198:232	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid.					
29758540	4	50	theme	membranes	895:903	arg1	activity					857:864	antifouling activity	845:864	antifouling activity	845:864	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	4	50	theme	membranes	895:903	arg1	hemocompatibility					870:886	hemocompatibility	870:886	hemocompatibility	870:886	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	5	51	theme	obvious	1175:1181	arg1	cytotoxicity					1183:1194	no obvious cytotoxicity	1172:1194	no obvious cytotoxicity	1172:1194	Moreover, cell culture assays indicated that the prepared membranes exerted no obvious cytotoxicity with more than 80% of relative L929 fibroblast viability.					
29758540	4	52	theme	platelet	997:1004	arg1	adhesion					1006:1013	platelet adhesion	997:1013	platelet adhesion	997:1013	The investigations on antifouling activity and hemocompatibility of the membranes showed significant resistances to bacterial attachment, non-specific protein adsorption and platelet adhesion, and presented lower hemolytic activity and good anticoagulant activity as well.					
29758540	2	53	theme	zwitterionic	430:441	arg1	methacrylate					502:513	sulfobetaine methylacrylate-co-2-aminoethyl methacrylate	458:513	sulfobetaine methylacrylate-co-2-aminoethyl methacrylate	458:513	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	2	53	theme	zwitterionic	430:441	arg1	poly					453:456	the zwitterionic copolymer poly	426:456	the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate)	426:514	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	1	54	theme	body	303:306	arg1	fluid					308:312	the body fluid	299:312	the body fluid	299:312	Integrated antibacterial and antifouling surfaces in favor of avoiding implant-related infections are necessarily required for biomaterials when they contact with the body fluid.					
29758540	2	55	theme	copolymer	443:451	arg1	methacrylate					502:513	sulfobetaine methylacrylate-co-2-aminoethyl methacrylate	458:513	sulfobetaine methylacrylate-co-2-aminoethyl methacrylate	458:513	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
29758540	2	55	theme	copolymer	443:451	arg1	poly					453:456	the zwitterionic copolymer poly	426:456	the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate)	426:514	In this work, an antibacterial and antifouling membrane was developed via cross-linking chitosan-g-eugenol and the zwitterionic copolymer poly(sulfobetaine methylacrylate-co-2-aminoethyl methacrylate) on the electrospun polycarbonate urethane substrate using genipin as a cross-linker.					
31678385	5	0	theme	Cet-	784:787	arg1	MCPCNPs					789:795	Cet- MCPCNPs	784:795	Cet- MCPCNPs	784:795	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	1	1	theme	modified	174:181	arg1	nanoparticles					206:218	a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles	151:218	a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer	151:305	In the present study, we successfully developed a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer.					
31678385	4	2	from	reduction	707:715	arg1	propagation					732:742	cancer cell propagation	720:742	cancer cell propagation	720:742	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	6	3	theme	colorectal	1012:1021	arg1	cancer					1023:1028	colorectal cancer	1012:1028	colorectal cancer	1012:1028	These data suggest that Cet-MCPCNPs represent a new and promising targeting approach for the treatment of colorectal cancer.					
31678385	1	4	theme	citrus	183:188	arg1	nanoparticles					206:218	a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles	151:218	a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer	151:305	In the present study, we successfully developed a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer.					
31678385	4	5	theme	enhanced	596:603	arg1	internalization					605:619	enhanced internalization	596:619	enhanced internalization of Cet-MCPCNPs	596:634	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	3	6	theme	nanoparticles	547:559	arg1	surface					532:538	the surface	528:538	the surface of the nanoparticles	528:559	61.37 ± 0.70% of cetuximab was adsorbed to the surface of the nanoparticles.					
31678385	1	7	theme	colorectal	289:298	arg1	cancer					300:305	colorectal cancer	289:305	colorectal cancer	289:305	In the present study, we successfully developed a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer.					
31678385	5	8	theme	cell	749:752	arg1	analysis					760:767	The cell cycle analysis	745:767	The cell cycle analysis	745:767	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	1	9	theme	cancer	300:305	arg1	treatment					276:284	the treatment	272:284	the treatment of colorectal cancer	272:305	In the present study, we successfully developed a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer.					
31678385	5	10	theme	cycle	754:758	arg1	analysis					760:767	The cell cycle analysis	745:767	The cell cycle analysis	745:767	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	4	11	theme	cell	727:730	arg1	propagation					732:742	cancer cell propagation	720:742	cancer cell propagation	720:742	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	5	12	theme	enhanced	819:826	arg1	percentage					828:837	enhanced percentage	819:837	enhanced percentage of Caco-2 cells	819:853	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	2	13	theme	In	308:309	arg1	analyses					317:324	In vitro analyses	308:324	In vitro analyses	308:324	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	1	14	theme	pectin-chitosan	190:204	arg1	nanoparticles					206:218	a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles	151:218	a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer	151:305	In the present study, we successfully developed a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer.					
31678385	2	15	theme	drug	463:466	arg1	profile					476:482	a 'smart' drug release profile	453:482	a 'smart' drug release profile	453:482	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	2	16	theme	efficiency	423:432	arg1	size					374:377	size	374:377	size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile	374:482	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	0	17	theme	Cetuximab-conjugated	0:19	arg1	composite					51:59	Cetuximab-conjugated chitosan-pectinate (modified) composite	0:59	Cetuximab-conjugated chitosan-pectinate (modified) composite	0:59	Cetuximab-conjugated chitosan-pectinate (modified) composite nanoparticles for targeting colon cancer.					
31678385	4	18	theme	uptake	571:576	arg1	studies					578:584	Cellular uptake studies	562:584	Cellular uptake studies	562:584	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	5	19	theme	cycle	874:878	arg1	arrest					880:885	cell cycle arrest	869:885	cell cycle arrest	869:885	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	2	20	theme	encapsulation	409:421	arg1	efficiency					423:432	a decent encapsulation efficiency	400:432	a decent encapsulation efficiency (68.43 ± 2.4%)	400:447	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	2	20	theme	encapsulation	409:421	arg1	%					446:446	68.43 ± 2.4%	435:446	68.43 ± 2.4%	435:446	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	0	21	theme	modified	41:48	arg1	composite					51:59	Cetuximab-conjugated chitosan-pectinate (modified) composite	0:59	Cetuximab-conjugated chitosan-pectinate (modified) composite	0:59	Cetuximab-conjugated chitosan-pectinate (modified) composite nanoparticles for targeting colon cancer.					
31678385	4	22	theme	Cellular	562:569	arg1	studies					578:584	Cellular uptake studies	562:584	Cellular uptake studies	562:584	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	1	23	theme	present	110:116	arg1	study					118:122	the present study	106:122	the present study	106:122	In the present study, we successfully developed a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer.					
31678385	5	24	theme	cells	849:853	arg1	percentage					828:837	enhanced percentage	819:837	enhanced percentage of Caco-2 cells	819:853	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	5	25	theme	Caco-2	842:847	arg1	cells					849:853	Caco-2 cells	842:853	Caco-2 cells	842:853	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	6	26	theme	targeting	972:980	arg1	approach					982:989	a new and promising targeting approach	952:989	a new and promising targeting approach for the treatment of colorectal cancer	952:1028	These data suggest that Cet-MCPCNPs represent a new and promising targeting approach for the treatment of colorectal cancer.					
31678385	2	27	theme	249.33 ± 5.15 nm	382:397	arg1	size					374:377	size	374:377	size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile	374:482	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	2	28	dep	In	308:309	arg1	vitro					311:315	vitro	311:315	vitro	311:315	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	6	29	theme	promising	962:970	arg1	approach					982:989	a new and promising targeting approach	952:989	a new and promising targeting approach for the treatment of colorectal cancer	952:1028	These data suggest that Cet-MCPCNPs represent a new and promising targeting approach for the treatment of colorectal cancer.					
31678385	2	30	with	spherical	359:367	arg1	size					374:377	size	374:377	size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile	374:482	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	1	31	theme	targeted	224:231	arg1	delivery					233:240	targeted delivery	224:240	targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer	224:305	In the present study, we successfully developed a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer.					
31678385	1	32	theme	curcumin	245:252	arg1	delivery					233:240	targeted delivery	224:240	targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer	224:305	In the present study, we successfully developed a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer.					
31678385	4	33	theme	significant	695:705	arg1	reduction					707:715	a significant reduction	693:715	a significant reduction in cancer cell propagation	693:742	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	5	34	theme	cell	805:808	arg1	death					810:814	cell death	805:814	cell death	805:814	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	2	35	theme	smart	456:460	arg1	profile					476:482	a 'smart' drug release profile	453:482	a 'smart' drug release profile	453:482	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	5	36	theme	cell	869:872	arg1	arrest					880:885	cell cycle arrest	869:885	cell cycle arrest	869:885	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	2	37	theme	profile	476:482	arg1	size					374:377	size	374:377	size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile	374:482	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	5	38	theme	G2/M	894:897	arg1	phase					899:903	the G2/M phase	890:903	the G2/M phase	890:903	The cell cycle analysis indicated that Cet- MCPCNPs induced cell death in enhanced percentage of Caco-2 cells by undergoing cell cycle arrest in the G2/M phase.					
31678385	3	39	theme	cetuximab	502:510	arg1	cetuximab					502:510	cetuximab	502:510	cetuximab	502:510	61.37 ± 0.70% of cetuximab was adsorbed to the surface of the nanoparticles.					
31678385	3	39	theme	cetuximab	502:510	arg1	%					497:497	61.37 ± 0.70%	485:497	61.37 ± 0.70% of cetuximab	485:510	61.37 ± 0.70% of cetuximab was adsorbed to the surface of the nanoparticles.					
31678385	2	40	theme	release	468:474	arg1	profile					476:482	a 'smart' drug release profile	453:482	a 'smart' drug release profile	453:482	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	4	41	theme	cancer	720:725	arg1	propagation					732:742	cancer cell propagation	720:742	cancer cell propagation	720:742	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	4	42	theme	Caco-2	639:644	arg1	cells					657:661	Caco-2 (EGFR +ve) cells	639:661	Caco-2 (EGFR +ve) cells	639:661	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	1	43	theme	cetuximab-conjugated	153:172	arg1	nanoparticles					206:218	a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles	151:218	a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer	151:305	In the present study, we successfully developed a cetuximab-conjugated modified citrus pectin-chitosan nanoparticles for targeted delivery of curcumin (Cet-MCPCNPs) for the treatment of colorectal cancer.					
31678385	0	44	theme	colon	89:93	arg1	cancer					95:100	colon cancer	89:100	colon cancer	89:100	Cetuximab-conjugated chitosan-pectinate (modified) composite nanoparticles for targeting colon cancer.					
31678385	2	45	theme	decent	402:407	arg1	efficiency					423:432	a decent encapsulation efficiency	400:432	a decent encapsulation efficiency (68.43 ± 2.4%)	400:447	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	2	45	theme	decent	402:407	arg1	%					446:446	68.43 ± 2.4%	435:446	68.43 ± 2.4%	435:446	In vitro analyses revealed that nanoparticles were spherical with size of 249.33 ± 5.15 nm, a decent encapsulation efficiency (68.43 ± 2.4%) and a 'smart' drug release profile.					
31678385	4	46	theme	EGFR	647:650	arg1	Caco-2					639:644	Caco-2	639:644	Caco-2 (EGFR +ve) cells	639:661	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	4	46	theme	EGFR	647:650	arg1	+ve					652:654	EGFR +ve	647:654	EGFR +ve	647:654	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	4	47	theme	Cet-MCPCNPs	624:634	arg1	internalization					605:619	enhanced internalization	596:619	enhanced internalization of Cet-MCPCNPs	596:634	Cellular uptake studies displayed enhanced internalization of Cet-MCPCNPs in Caco-2 (EGFR +ve) cells, which ultimately resulted in a significant reduction in cancer cell propagation.					
31678385	0	48	theme	chitosan-pectinate	21:38	arg1	composite					51:59	Cetuximab-conjugated chitosan-pectinate (modified) composite	0:59	Cetuximab-conjugated chitosan-pectinate (modified) composite	0:59	Cetuximab-conjugated chitosan-pectinate (modified) composite nanoparticles for targeting colon cancer.					
31678385	6	49	theme	new	954:956	arg1	approach					982:989	a new and promising targeting approach	952:989	a new and promising targeting approach for the treatment of colorectal cancer	952:1028	These data suggest that Cet-MCPCNPs represent a new and promising targeting approach for the treatment of colorectal cancer.					
31678385	6	50	theme	cancer	1023:1028	arg1	treatment					999:1007	the treatment	995:1007	the treatment of colorectal cancer	995:1028	These data suggest that Cet-MCPCNPs represent a new and promising targeting approach for the treatment of colorectal cancer.					
30964694	0	0	theme	Antiviral	65:73	arg1	Immunity					75:82	Antiviral Immunity	65:82	Antiviral Immunity	65:82	Glucan Particles Are a Powerful Adjuvant for the HBsAg, Favoring Antiviral Immunity.					
30964694	3	1	theme	particles	298:306	arg1	activity					272:279	The adjuvant activity	259:279	The adjuvant activity of pathogen-like particles	259:306	The adjuvant activity of pathogen-like particles is highly influenced by size and surface composition.					
30964694	9	2	theme	only	1256:1259	arg1	GPs					1261:1263	only GPs	1256:1263	only GPs	1256:1263	Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines.					
30964694	8	3	theme	B	1073:1073	arg1	HBsAg					1092:1096	HBsAg	1092:1096	HBsAg	1092:1096	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	8	3	theme	B	1073:1073	arg1	antigen					1083:1089	the hepatitis B surface antigen	1059:1089	the hepatitis B surface antigen (HBsAg)	1059:1097	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	6	4	theme	RANTES	819:824	arg1	production					789:798	the production	785:798	the production of either TNF-α or RANTES	785:824	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	4	5	theme	nm	559:560	arg1	size					546:549	mean size	541:549	mean size of 1276 nm	541:560	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	6	6	theme	in	837:838	arg1	stimulation					846:856	in vitro stimulation	837:856	in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs)	837:950	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	4	7	theme	μm	635:636	arg1	size					625:628	a mean size	618:628	a mean size of 3 μm	618:636	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	7	8	theme	Human	953:957	arg1	monocytes					959:967	Human monocytes	953:967	Human monocytes	953:967	Human monocytes responded to all particles through TNF-α secretion.					
30964694	10	9	theme	significant	1426:1436	arg1	role					1438:1441	a significant role	1424:1441	a significant role	1424:1441	This study demonstrates, for the first time, that GPs can have a significant role against the hepatitis B virus by favoring antiviral immunity.					
30964694	3	10	theme	surface	341:347	arg1	composition					349:359	surface composition	341:359	surface composition	341:359	The adjuvant activity of pathogen-like particles is highly influenced by size and surface composition.					
30964694	4	11	theme	chitosan/β-glucan	463:479	arg1	particles					481:489	blend chitosan/β-glucan particles	457:489	blend chitosan/β-glucan particles (ChiGluPs)	457:500	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	4	11	theme	chitosan/β-glucan	463:479	arg1	ChiGluPs					492:499	ChiGluPs	492:499	ChiGluPs	492:499	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	10	12	theme	B	1465:1465	arg1	virus					1467:1471	the hepatitis B virus	1451:1471	the hepatitis B virus	1451:1471	This study demonstrates, for the first time, that GPs can have a significant role against the hepatitis B virus by favoring antiviral immunity.					
30964694	6	13	dep	in	837:838	arg1	vitro					840:844	vitro	840:844	vitro	840:844	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	4	14	theme	blend	457:461	arg1	particles					481:489	blend chitosan/β-glucan particles	457:489	blend chitosan/β-glucan particles (ChiGluPs)	457:500	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	4	14	theme	blend	457:461	arg1	ChiGluPs					492:499	ChiGluPs	492:499	ChiGluPs	492:499	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	7	15	theme	TNF-α	1004:1008	arg1	secretion					1010:1018	TNF-α secretion	1004:1018	TNF-α secretion	1004:1018	Human monocytes responded to all particles through TNF-α secretion.					
30964694	8	16	with	vaccination	1034:1044	arg1	HBsAg					1092:1096	HBsAg	1092:1096	HBsAg	1092:1096	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	8	16	with	vaccination	1034:1044	arg1	antigen					1083:1089	the hepatitis B surface antigen	1059:1089	the hepatitis B surface antigen (HBsAg)	1059:1097	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	9	17	theme	HBsAg-specific	1291:1304	arg1	Th1					1306:1308	HBsAg-specific Th1	1291:1308	HBsAg-specific Th1	1291:1308	Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines.					
30964694	9	18	theme	Th2	1311:1313	arg1	secretion					1278:1286	the secretion	1274:1286	the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines	1274:1358	Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines.					
30964694	1	19	theme	robust	139:144	arg1	responses					153:161	robust T cell responses	139:161	robust T cell responses	139:161	The lack of vaccine adjuvants that are able to induce robust T cell responses fosters the search for more powerful options.					
30964694	5	20	theme	addition	727:734	arg1	effect					708:713	the effect	704:713	the effect of β-glucan addition (ChiGluPs)	704:745	Additionally, chitosan particles (ChiPs) were used to understand the effect of β-glucan addition (ChiGluPs).					
30964694	6	21	theme	TNF-α	810:814	arg1	production					789:798	the production	785:798	the production of either TNF-α or RANTES	785:824	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	2	22	theme	promising	239:247	arg1	particles					223:231	Pathogen-like particles	209:231	Pathogen-like particles	209:231	Pathogen-like particles are a promising approach.					
30964694	2	22	theme	promising	239:247	arg1	approach					249:256	a promising approach	237:256	a promising approach	237:256	Pathogen-like particles are a promising approach.					
30964694	1	23	theme	T	146:146	arg1	responses					153:161	robust T cell responses	139:161	robust T cell responses	139:161	The lack of vaccine adjuvants that are able to induce robust T cell responses fosters the search for more powerful options.					
30964694	4	24	theme	particles	481:489	arg1	potential					404:412	the adjuvant potential	391:412	the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs)	391:610	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	8	25	dep	HBsAg	1156:1160	arg1	4500-					1175:1179	4500-	1175:1179	4500-	1175:1179	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	8	25	dep	HBsAg	1156:1160	arg1	435-					1169:1172	435-	1169:1172	435-	1169:1172	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	8	25	dep	HBsAg	1156:1160	arg1	increase					1195:1202	2500-fold increase	1185:1202	2500-fold increase for either ChiPs, ChiGluPs, or GPs	1185:1237	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	6	26	dep	chitosan	922:929	arg1	ChiPs					945:949	ChiPs	945:949	ChiPs	945:949	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	6	26	dep	chitosan	922:929	arg1	ChiGluPs					932:939	ChiGluPs	932:939	ChiGluPs	932:939	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	1	27	theme	cell	148:151	arg1	responses					153:161	robust T cell responses	139:161	robust T cell responses	139:161	The lack of vaccine adjuvants that are able to induce robust T cell responses fosters the search for more powerful options.					
30964694	0	28	theme	Glucan	0:5	arg1	Adjuvant					32:39	Adjuvant	32:39	Adjuvant	32:39	Glucan Particles Are a Powerful Adjuvant for the HBsAg, Favoring Antiviral Immunity.					
30964694	0	28	theme	Glucan	0:5	arg1	Particles					7:15	Glucan Particles	0:15	Glucan Particles	0:15	Glucan Particles Are a Powerful Adjuvant for the HBsAg, Favoring Antiviral Immunity.					
30964694	4	29	theme	mean	541:544	arg1	size					546:549	mean size	541:549	mean size of 1276 nm	541:560	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	5	30	used	used	685:688	arg2	particles					662:670	chitosan particles	653:670	chitosan particles (ChiPs)	653:678	Additionally, chitosan particles (ChiPs) were used to understand the effect of β-glucan addition (ChiGluPs).					
30964694	5	30	used	used	685:688	arg2	ChiPs					673:677	ChiPs	673:677	ChiPs	673:677	Additionally, chitosan particles (ChiPs) were used to understand the effect of β-glucan addition (ChiGluPs).					
30964694	6	31	with	stimulation	846:856	arg1	particles					863:871	particles	863:871	particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs)	863:950	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	8	32	theme	hepatitis	1063:1071	arg1	HBsAg					1092:1096	HBsAg	1092:1096	HBsAg	1092:1096	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	8	32	theme	hepatitis	1063:1071	arg1	antigen					1083:1089	the hepatitis B surface antigen	1059:1089	the hepatitis B surface antigen (HBsAg)	1059:1097	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	4	33	theme	mean	620:623	arg1	size					625:628	a mean size	618:628	a mean size of 3 μm	618:636	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	8	34	theme	increased	1106:1114	arg1	IgG					1122:1124	increased serum IgG	1106:1124	increased serum IgG for all particles	1106:1142	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	8	35	theme	2500-fold	1185:1193	arg1	increase					1195:1202	2500-fold increase	1185:1202	2500-fold increase for either ChiPs, ChiGluPs, or GPs	1185:1237	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	9	36	theme	Th1	1306:1308	arg1	secretion					1278:1286	the secretion	1274:1286	the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines	1274:1358	Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines.					
30964694	4	37	contain	have	536:539	arg1	particles					481:489	blend chitosan/β-glucan particles	457:489	blend chitosan/β-glucan particles (ChiGluPs)	457:500	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	4	37	contain	have	536:539	arg2	size					546:549	mean size	541:549	mean size of 1276 nm	541:560	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	4	37	contain	have	536:539	arg1	ChiGluPs					492:499	ChiGluPs	492:499	ChiGluPs	492:499	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	9	38	theme	Th9	1316:1318	arg1	secretion					1278:1286	the secretion	1274:1286	the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines	1274:1358	Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines.					
30964694	4	39	theme	β-glucan-based	431:444	arg1	particles					446:454	two different β-glucan-based particles	417:454	two different β-glucan-based particles	417:454	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	5	40	theme	chitosan	653:660	arg1	ChiPs					673:677	ChiPs	673:677	ChiPs	673:677	Additionally, chitosan particles (ChiPs) were used to understand the effect of β-glucan addition (ChiGluPs).					
30964694	5	40	theme	chitosan	653:660	arg1	particles					662:670	chitosan particles	653:670	chitosan particles (ChiPs)	653:678	Additionally, chitosan particles (ChiPs) were used to understand the effect of β-glucan addition (ChiGluPs).					
30964694	4	41	theme	different	421:429	arg1	particles					446:454	two different β-glucan-based particles	417:454	two different β-glucan-based particles	417:454	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	8	42	dep	showed	1099:1104	arg1	compared					1144:1151	compared	1144:1151	showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs)	1099:1238	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	10	43	theme	hepatitis	1455:1463	arg1	virus					1467:1471	the hepatitis B virus	1451:1471	the hepatitis B virus	1451:1471	This study demonstrates, for the first time, that GPs can have a significant role against the hepatitis B virus by favoring antiviral immunity.					
30964694	9	44	theme	Th17	1321:1324	arg1	secretion					1278:1286	the secretion	1274:1286	the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines	1274:1358	Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines.					
30964694	8	45	theme	serum	1116:1120	arg1	IgG					1122:1124	increased serum IgG	1106:1124	increased serum IgG for all particles	1106:1142	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	4	46	link	yeast-derived	575:587	arg1	GPs					607:609	GPs	607:609	GPs	607:609	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	4	46	link	yeast-derived	575:587	arg1	particles					596:604	neutral yeast-derived glucan particles	567:604	neutral yeast-derived glucan particles (GPs)	567:610	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	10	47	theme	first	1394:1398	arg1	time					1400:1403	the first time	1390:1403	the first time	1390:1403	This study demonstrates, for the first time, that GPs can have a significant role against the hepatitis B virus by favoring antiviral immunity.					
30964694	5	48	theme	β-glucan	718:725	arg1	ChiGluPs					737:744	ChiGluPs	737:744	ChiGluPs	737:744	Additionally, chitosan particles (ChiPs) were used to understand the effect of β-glucan addition (ChiGluPs).					
30964694	5	48	theme	β-glucan	718:725	arg1	addition					727:734	β-glucan addition	718:734	β-glucan addition (ChiGluPs)	718:745	Additionally, chitosan particles (ChiPs) were used to understand the effect of β-glucan addition (ChiGluPs).					
30964694	8	49	theme	surface	1075:1081	arg1	HBsAg					1092:1096	HBsAg	1092:1096	HBsAg	1092:1096	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	8	49	theme	surface	1075:1081	arg1	antigen					1083:1089	the hepatitis B surface antigen	1059:1089	the hepatitis B surface antigen (HBsAg)	1059:1097	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	8	50	theme	Subcutaneous	1021:1032	arg1	vaccination					1034:1044	Subcutaneous vaccination	1021:1044	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg)	1021:1097	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	4	51	theme	particles	446:454	arg1	potential					404:412	the adjuvant potential	391:412	the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs)	391:610	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	10	52	contain	have	1419:1422	arg1	GPs					1411:1413	GPs	1411:1413	GPs	1411:1413	This study demonstrates, for the first time, that GPs can have a significant role against the hepatitis B virus by favoring antiviral immunity.					
30964694	10	52	contain	have	1419:1422	arg2	role					1438:1441	a significant role	1424:1441	a significant role	1424:1441	This study demonstrates, for the first time, that GPs can have a significant role against the hepatitis B virus by favoring antiviral immunity.					
30964694	9	53	theme	Th22	1327:1330	arg1	secretion					1278:1286	the secretion	1274:1286	the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines	1274:1358	Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines.					
30964694	4	54	theme	neutral	567:573	arg1	GPs					607:609	GPs	607:609	GPs	607:609	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	4	54	theme	neutral	567:573	arg1	particles					596:604	neutral yeast-derived glucan particles	567:604	neutral yeast-derived glucan particles (GPs)	567:610	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	3	55	theme	pathogen-like	284:296	arg1	particles					298:306	pathogen-like particles	284:306	pathogen-like particles	284:306	The adjuvant activity of pathogen-like particles is highly influenced by size and surface composition.					
30964694	6	56	dep	β-glucan	891:898	arg1	GPs					914:916	GPs	914:916	GPs	914:916	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	6	56	dep	β-glucan	891:898	arg1	ChiGluPs					901:908	ChiGluPs	901:908	ChiGluPs	901:908	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	1	57	theme	vaccine	97:103	arg1	adjuvants					105:113	vaccine adjuvants	97:113	vaccine adjuvants that are able to induce robust T cell responses	97:161	The lack of vaccine adjuvants that are able to induce robust T cell responses fosters the search for more powerful options.					
30964694	6	58	contain	containing	873:882	arg1	particles					863:871	particles	863:871	particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs)	863:950	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	6	58	contain	containing	873:882	arg2	β-glucan					891:898	β-glucan	891:898	β-glucan (ChiGluPs and GPs)	891:917	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	6	58	contain	containing	873:882	arg2	chitosan					922:929	chitosan	922:929	chitosan (ChiGluPs and ChiPs)	922:950	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	3	59	theme	adjuvant	263:270	arg1	activity					272:279	The adjuvant activity	259:279	The adjuvant activity of pathogen-like particles	259:306	The adjuvant activity of pathogen-like particles is highly influenced by size and surface composition.					
30964694	4	60	theme	adjuvant	395:402	arg1	potential					404:412	the adjuvant potential	391:412	the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs)	391:610	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	1	61	theme	adjuvants	105:113	arg1	lack					89:92	The lack	85:92	The lack of vaccine adjuvants that are able to induce robust T cell responses	85:161	The lack of vaccine adjuvants that are able to induce robust T cell responses fosters the search for more powerful options.					
30964694	4	62	theme	yeast-derived	575:587	arg1	GPs					607:609	GPs	607:609	GPs	607:609	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	4	62	theme	yeast-derived	575:587	arg1	particles					596:604	neutral yeast-derived glucan particles	567:604	neutral yeast-derived glucan particles (GPs)	567:610	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	10	63	theme	antiviral	1485:1493	arg1	immunity					1495:1502	antiviral immunity	1485:1502	antiviral immunity	1485:1502	This study demonstrates, for the first time, that GPs can have a significant role against the hepatitis B virus by favoring antiviral immunity.					
30964694	6	64	theme	spleen	754:759	arg1	cells					761:765	Mouse spleen cells	748:765	Mouse spleen cells	748:765	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	9	65	theme	Treg-related	1337:1348	arg1	cytokines					1350:1358	Treg-related cytokines	1337:1358	Treg-related cytokines	1337:1358	Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines.					
30964694	1	66	theme	powerful	191:198	arg1	options					200:206	more powerful options	186:206	more powerful options	186:206	The lack of vaccine adjuvants that are able to induce robust T cell responses fosters the search for more powerful options.					
30964694	6	67	theme	Mouse	748:752	arg1	cells					761:765	Mouse spleen cells	748:765	Mouse spleen cells	748:765	Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs).					
30964694	9	68	theme	cytokines	1350:1358	arg1	secretion					1278:1286	the secretion	1274:1286	the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines	1274:1358	Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22, and Treg-related cytokines.					
30964694	4	69	theme	particles	596:604	arg1	potential					404:412	the adjuvant potential	391:412	the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs)	391:610	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	8	70	theme	mice	1049:1052	arg1	vaccination					1034:1044	Subcutaneous vaccination	1021:1044	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg)	1021:1097	Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-, 4500-, or 2500-fold increase for either ChiPs, ChiGluPs, or GPs).					
30964694	2	71	theme	Pathogen-like	209:221	arg1	particles					223:231	Pathogen-like particles	209:231	Pathogen-like particles	209:231	Pathogen-like particles are a promising approach.					
30964694	2	71	theme	Pathogen-like	209:221	arg1	approach					249:256	a promising approach	237:256	a promising approach	237:256	Pathogen-like particles are a promising approach.					
30964694	4	72	theme	glucan	589:594	arg1	GPs					607:609	GPs	607:609	GPs	607:609	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30964694	4	72	theme	glucan	589:594	arg1	particles					596:604	neutral yeast-derived glucan particles	567:604	neutral yeast-derived glucan particles (GPs)	567:610	This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles, blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs), with a mean size of 3 μm.					
30242779	15	0	theme	asymmetric	1617:1626	arg1	udder					1628:1632	asymmetric udder	1617:1632	asymmetric udder	1617:1632	Ewes with asymmetric udder produced less and more concentrated milk than ewes with symmetrical udder.					
30242779	5	1	theme	Fleischmann	622:632	arg1	method					634:639	the Fleischmann method	618:639	the Fleischmann method	618:639	Total productions over lactation (milk, fat, and protein) were estimated using the Fleischmann method.					
30242779	2	2	theme	man	322:324	arg1	ewes					326:329	eighty lactating D'man ewes	303:329	eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia	303:392	Observations were performed in eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia.					
30242779	2	3	theme	intensive	351:359	arg1	system					367:372	an intensive oases system	348:372	an intensive oases system in Southern Tunisia	348:392	Observations were performed in eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia.					
30242779	7	4	theme	tenth	788:792	arg1	week					794:797	the tenth week	784:797	the tenth week	784:797	It increased to a plateau from the third to the fourth week of lactation, and decreased gradually to the tenth week.					
30242779	15	5	theme	concentrated	1657:1668	arg1	milk					1670:1673	less and more concentrated milk	1643:1673	less and more concentrated milk	1643:1673	Ewes with asymmetric udder produced less and more concentrated milk than ewes with symmetrical udder.					
30242779	14	6	theme	older	1502:1506	arg1	ewes					1508:1511	younger and older ewes	1490:1511	younger and older ewes	1490:1511	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	1	7	theme	factors	193:199	arg1	potentiality					142:153	milk potentiality	137:153	milk potentiality	137:153	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	1	7	theme	factors	193:199	arg1	influence					163:171	the influence	159:171	the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep	159:269	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	0	8	from	lactation	54:62	arg1	oases					99:103	Tunisian oases	90:103	Tunisian oases	90:103	Factors affecting the milk yield and composition over lactation of prolific D'man ewes in Tunisian oases.					
30242779	9	9	theme	solids	946:951	arg1	lactose					996:1002	lactose	996:1002	lactose	996:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	%					958:958	7.08%	954:958	7.08% of fat	954:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	proteins					977:984	proteins	977:984	proteins	977:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	%					935:935	16.17%	930:935	16.17% of total solids	930:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	%					1038:1038	0.73%	1034:1038	0.73% of ash	1034:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	%					1009:1009	9.09%	1005:1009	9.09% of solids-not-fat	1005:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	fat					963:965	fat	963:965	fat	963:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	solids					946:951	total solids	940:951	total solids	940:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	solids-not-fat					1014:1027	solids-not-fat	1014:1027	solids-not-fat	1014:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	ash					1043:1045	ash	1043:1045	ash	1043:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	%					991:991	4.32%	987:991	4.32% of lactose	987:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	9	theme	solids	946:951	arg1	%					972:972	4.04%	968:972	4.04% of proteins	968:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	14	10	theme	younger	1490:1496	arg1	ewes					1508:1511	younger and older ewes	1490:1511	younger and older ewes	1490:1511	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	13	11	theme	milk	1383:1386	arg1	proteins					1394:1401	milk total proteins	1383:1401	milk total proteins	1383:1401	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	10	12	theme	milk	1082:1085	arg1	yield					1087:1091	the daily milk yield	1072:1091	the daily milk yield	1072:1091	Lambing season affected the daily milk yield and milk composition, where fat and total proteins were highest in autumn.					
30242779	14	13	theme	more	1468:1471	arg1	milk					1473:1476	more milk	1468:1476	more milk	1468:1476	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	8	14	theme	D'	800:801	arg1	ewes					806:809	D'man ewes	800:809	D'man ewes	800:809	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	0	15	theme	prolific	67:74	arg1	ewes					82:85	prolific D'man ewes	67:85	prolific D'man ewes	67:85	Factors affecting the milk yield and composition over lactation of prolific D'man ewes in Tunisian oases.					
30242779	1	16	theme	milk	137:140	arg1	potentiality					142:153	milk potentiality	137:153	milk potentiality	137:153	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	4	17	theme	milk	493:496	arg1	composition					498:508	Chemical milk composition	484:508	Chemical milk composition	484:508	Chemical milk composition was determined by Lactoscan.					
30242779	17	18	theme	total	1907:1911	arg1	proteins					1913:1920	total proteins	1907:1920	total proteins	1907:1920	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	0	19	theme	man	78:80	arg1	ewes					82:85	prolific D'man ewes	67:85	prolific D'man ewes	67:85	Factors affecting the milk yield and composition over lactation of prolific D'man ewes in Tunisian oases.					
30242779	9	20	theme	lactose	996:1002	arg1	lactose					996:1002	lactose	996:1002	lactose	996:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	%					958:958	7.08%	954:958	7.08% of fat	954:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	proteins					977:984	proteins	977:984	proteins	977:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	%					935:935	16.17%	930:935	16.17% of total solids	930:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	%					1038:1038	0.73%	1034:1038	0.73% of ash	1034:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	%					1009:1009	9.09%	1005:1009	9.09% of solids-not-fat	1005:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	fat					963:965	fat	963:965	fat	963:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	solids					946:951	total solids	940:951	total solids	940:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	solids-not-fat					1014:1027	solids-not-fat	1014:1027	solids-not-fat	1014:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	ash					1043:1045	ash	1043:1045	ash	1043:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	%					991:991	4.32%	987:991	4.32% of lactose	987:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	20	theme	lactose	996:1002	arg1	%					972:972	4.04%	968:972	4.04% of proteins	968:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	lactose					996:1002	lactose	996:1002	lactose	996:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	%					958:958	7.08%	954:958	7.08% of fat	954:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	proteins					977:984	proteins	977:984	proteins	977:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	%					935:935	16.17%	930:935	16.17% of total solids	930:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	%					1038:1038	0.73%	1034:1038	0.73% of ash	1034:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	%					1009:1009	9.09%	1005:1009	9.09% of solids-not-fat	1005:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	fat					963:965	fat	963:965	fat	963:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	solids					946:951	total solids	940:951	total solids	940:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	solids-not-fat					1014:1027	solids-not-fat	1014:1027	solids-not-fat	1014:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	ash					1043:1045	ash	1043:1045	ash	1043:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	%					991:991	4.32%	987:991	4.32% of lactose	987:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	21	theme	proteins	977:984	arg1	%					972:972	4.04%	968:972	4.04% of proteins	968:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	16	22	theme	total	1755:1759	arg1	solids					1761:1766	total solids	1755:1766	total solids	1755:1766	Daily milk yield was negatively correlated to total solids, fat, and total proteins amounts.					
30242779	8	23	theme	milk	839:842	arg1	5.26 ± 0.12 kg					872:885	5.26 ± 0.12 kg	872:885	5.26 ± 0.12 kg of total proteins	872:903	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	8	23	theme	milk	839:842	arg1	9.23 ± 0.26 kg					845:858	9.23 ± 0.26 kg	845:858	9.23 ± 0.26 kg of fat	845:865	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	8	23	theme	milk	839:842	arg1	128.91 ± 3.14 l					820:834	128.91 ± 3.14 l	820:834	128.91 ± 3.14 l of milk	820:842	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	15	24	theme	symmetrical	1690:1700	arg1	udder					1702:1706	symmetrical udder	1690:1706	symmetrical udder	1690:1706	Ewes with asymmetric udder produced less and more concentrated milk than ewes with symmetrical udder.					
30242779	3	25	theme	Daily	395:399	arg1	yield					406:410	Daily milk yield	395:410	Daily milk yield	395:410	Daily milk yield was determined at 7-day intervals using oxytocin + hand milking method.					
30242779	1	26	theme	prolific	250:257	arg1	sheep					265:269	prolific D'man sheep	250:269	prolific D'man sheep	250:269	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	16	27	theme	proteins	1784:1791	arg1	amounts					1793:1799	total proteins amounts	1778:1799	total proteins amounts	1778:1799	Daily milk yield was negatively correlated to total solids, fat, and total proteins amounts.					
30242779	3	28	theme	7-day	430:434	arg1	intervals					436:444	7-day intervals	430:444	7-day intervals using oxytocin + hand milking method	430:481	Daily milk yield was determined at 7-day intervals using oxytocin + hand milking method.					
30242779	1	29	theme	man	261:263	arg1	sheep					265:269	prolific D'man sheep	250:269	prolific D'man sheep	250:269	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	8	30	theme	fat	863:865	arg1	5.26 ± 0.12 kg					872:885	5.26 ± 0.12 kg	872:885	5.26 ± 0.12 kg of total proteins	872:903	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	8	30	theme	fat	863:865	arg1	9.23 ± 0.26 kg					845:858	9.23 ± 0.26 kg	845:858	9.23 ± 0.26 kg of fat	845:865	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	8	30	theme	fat	863:865	arg1	128.91 ± 3.14 l					820:834	128.91 ± 3.14 l	820:834	128.91 ± 3.14 l of milk	820:842	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	6	31	theme	Daily	642:646	arg1	yield					653:657	Daily milk yield	642:657	Daily milk yield	642:657	Daily milk yield averaged 1.64 ± 0.02 l.					
30242779	2	32	theme	Southern	377:384	arg1	Tunisia					386:392	Southern Tunisia	377:392	Southern Tunisia	377:392	Observations were performed in eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia.					
30242779	14	33	theme	ash	1569:1571	arg1	levels					1573:1578	ash levels	1569:1578	ash levels	1569:1578	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	9	34	contain	contained	920:928	arg2	solids					946:951	total solids	940:951	total solids	940:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	fat					963:965	fat	963:965	fat	963:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	%					1009:1009	9.09%	1005:1009	9.09% of solids-not-fat	1005:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	%					972:972	4.04%	968:972	4.04% of proteins	968:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	solids-not-fat					1014:1027	solids-not-fat	1014:1027	solids-not-fat	1014:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	%					991:991	4.32%	987:991	4.32% of lactose	987:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	ash					1043:1045	ash	1043:1045	ash	1043:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	%					935:935	16.17%	930:935	16.17% of total solids	930:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	lactose					996:1002	lactose	996:1002	lactose	996:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	%					1038:1038	0.73%	1034:1038	0.73% of ash	1034:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg1	milk					915:918	milk	915:918	milk	915:918	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	proteins					977:984	proteins	977:984	proteins	977:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	34	contain	contained	920:928	arg2	%					958:958	7.08%	954:958	7.08% of fat	954:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	17	35	theme	total	1886:1890	arg1	milk					1892:1895	total milk	1886:1895	total milk	1886:1895	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	3	36	theme	milking	468:474	arg1	method					476:481	oxytocin + hand milking method	452:481	oxytocin + hand milking method	452:481	Daily milk yield was determined at 7-day intervals using oxytocin + hand milking method.					
30242779	7	37	theme	fourth	731:736	arg1	week					738:741	the fourth week	727:741	the fourth week of lactation	727:754	It increased to a plateau from the third to the fourth week of lactation, and decreased gradually to the tenth week.					
30242779	15	38	with	Ewes	1607:1610	arg1	udder					1628:1632	asymmetric udder	1617:1632	asymmetric udder	1617:1632	Ewes with asymmetric udder produced less and more concentrated milk than ewes with symmetrical udder.					
30242779	2	39	from	system	367:372	arg1	Tunisia					386:392	Southern Tunisia	377:392	Southern Tunisia	377:392	Observations were performed in eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	lactose					996:1002	lactose	996:1002	lactose	996:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	%					958:958	7.08%	954:958	7.08% of fat	954:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	proteins					977:984	proteins	977:984	proteins	977:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	%					935:935	16.17%	930:935	16.17% of total solids	930:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	%					1038:1038	0.73%	1034:1038	0.73% of ash	1034:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	%					1009:1009	9.09%	1005:1009	9.09% of solids-not-fat	1005:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	fat					963:965	fat	963:965	fat	963:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	solids					946:951	total solids	940:951	total solids	940:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	solids-not-fat					1014:1027	solids-not-fat	1014:1027	solids-not-fat	1014:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	ash					1043:1045	ash	1043:1045	ash	1043:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	%					991:991	4.32%	987:991	4.32% of lactose	987:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	40	theme	solids-not-fat	1014:1027	arg1	%					972:972	4.04%	968:972	4.04% of proteins	968:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	2	41	theme	eighty	303:308	arg1	ewes					326:329	eighty lactating D'man ewes	303:329	eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia	303:392	Observations were performed in eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia.					
30242779	10	42	theme	Lambing	1048:1054	arg1	season					1056:1061	Lambing season	1048:1061	Lambing season	1048:1061	Lambing season affected the daily milk yield and milk composition, where fat and total proteins were highest in autumn.					
30242779	16	43	theme	milk	1715:1718	arg1	yield					1720:1724	Daily milk yield	1709:1724	Daily milk yield	1709:1724	Daily milk yield was negatively correlated to total solids, fat, and total proteins amounts.					
30242779	8	44	theme	proteins	896:903	arg1	5.26 ± 0.12 kg					872:885	5.26 ± 0.12 kg	872:885	5.26 ± 0.12 kg of total proteins	872:903	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	8	44	theme	proteins	896:903	arg1	9.23 ± 0.26 kg					845:858	9.23 ± 0.26 kg	845:858	9.23 ± 0.26 kg of fat	845:865	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	8	44	theme	proteins	896:903	arg1	128.91 ± 3.14 l					820:834	128.91 ± 3.14 l	820:834	128.91 ± 3.14 l of milk	820:842	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	13	45	dep	lowest	1412:1417	arg1	levels					1440:1445	fat levels	1436:1445	fat levels	1436:1445	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	13	45	dep	lowest	1412:1417	arg1	solids					1425:1430	total solids	1419:1430	total solids	1419:1430	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	10	46	theme	fat	1121:1123	arg1	proteins					1135:1142	fat and total proteins	1121:1142	fat and total proteins	1121:1142	Lambing season affected the daily milk yield and milk composition, where fat and total proteins were highest in autumn.					
30242779	14	47	theme	total	1546:1550	arg1	solids					1552:1557	higher total solids	1539:1557	higher total solids	1539:1557	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	12	48	theme	multiple	1276:1283	arg1	lambs					1285:1289	multiple lambs	1276:1289	multiple lambs	1276:1289	Ewes reared multiple lambs produced more milk than those reared singles.					
30242779	10	49	theme	daily	1076:1080	arg1	yield					1087:1091	the daily milk yield	1072:1091	the daily milk yield	1072:1091	Lambing season affected the daily milk yield and milk composition, where fat and total proteins were highest in autumn.					
30242779	13	50	dep	proteins	1394:1401	arg1	the					1371:1373	the	1371:1373	the	1371:1373	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	5	51	dep	lactation	562:570	arg1	milk					573:576	milk	573:576	milk	573:576	Total productions over lactation (milk, fat, and protein) were estimated using the Fleischmann method.					
30242779	5	51	dep	lactation	562:570	arg1	fat					579:581	fat	579:581	fat	579:581	Total productions over lactation (milk, fat, and protein) were estimated using the Fleischmann method.					
30242779	5	51	dep	lactation	562:570	arg1	protein					588:594	protein	588:594	protein	588:594	Total productions over lactation (milk, fat, and protein) were estimated using the Fleischmann method.					
30242779	9	52	theme	ash	1043:1045	arg1	lactose					996:1002	lactose	996:1002	lactose	996:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	%					958:958	7.08%	954:958	7.08% of fat	954:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	proteins					977:984	proteins	977:984	proteins	977:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	%					935:935	16.17%	930:935	16.17% of total solids	930:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	%					1038:1038	0.73%	1034:1038	0.73% of ash	1034:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	%					1009:1009	9.09%	1005:1009	9.09% of solids-not-fat	1005:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	fat					963:965	fat	963:965	fat	963:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	solids					946:951	total solids	940:951	total solids	940:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	solids-not-fat					1014:1027	solids-not-fat	1014:1027	solids-not-fat	1014:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	ash					1043:1045	ash	1043:1045	ash	1043:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	%					991:991	4.32%	987:991	4.32% of lactose	987:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	52	theme	ash	1043:1045	arg1	%					972:972	4.04%	968:972	4.04% of proteins	968:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	2	53	theme	D'	320:321	arg1	ewes					326:329	eighty lactating D'man ewes	303:329	eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia	303:392	Observations were performed in eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia.					
30242779	13	54	theme	total	1419:1423	arg1	solids					1425:1430	total solids	1419:1430	total solids	1419:1430	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	13	55	contain	had	1367:1369	arg2	lowest					1412:1417	lowest	1412:1417	lowest	1412:1417	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	13	55	contain	had	1367:1369	arg2	proteins					1394:1401	milk total proteins	1383:1401	milk total proteins	1383:1401	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	13	55	contain	had	1367:1369	arg1	Dams					1337:1340	Dams	1337:1340	Dams nursing triplets or more	1337:1365	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	13	56	theme	total	1388:1392	arg1	proteins					1394:1401	milk total proteins	1383:1401	milk total proteins	1383:1401	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	11	57	theme	Total	1168:1172	arg1	solids					1174:1179	Total solids	1168:1179	Total solids	1168:1179	Total solids, lactose, solids-not-fat, and ash were higher in winter than in autumn and summer.					
30242779	1	58	from	composition	235:245	arg1	sheep					265:269	prolific D'man sheep	250:269	prolific D'man sheep	250:269	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	1	58	from	composition	235:245	arg1	yield					214:218	milk yield	209:218	milk yield	209:218	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	14	59	theme	young	1595:1599	arg1	ewes					1601:1604	young ewes	1595:1604	young ewes	1595:1604	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	1	60	from	production	220:229	arg1	sheep					265:269	prolific D'man sheep	250:269	prolific D'man sheep	250:269	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	1	60	from	production	220:229	arg1	yield					214:218	milk yield	209:218	milk yield	209:218	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	1	61	theme	non-genetic	181:191	arg1	factors					193:199	some non-genetic factors	176:199	some non-genetic factors	176:199	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	9	62	theme	total	940:944	arg1	solids					946:951	total solids	940:951	total solids	940:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	13	63	theme	highest	1375:1381	arg1	proteins					1394:1401	milk total proteins	1383:1401	milk total proteins	1383:1401	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	5	64	theme	Total	539:543	arg1	productions					545:555	Total productions	539:555	Total productions over lactation (milk, fat, and protein)	539:595	Total productions over lactation (milk, fat, and protein) were estimated using the Fleischmann method.					
30242779	10	65	theme	milk	1097:1100	arg1	composition					1102:1112	milk composition	1097:1112	milk composition	1097:1112	Lambing season affected the daily milk yield and milk composition, where fat and total proteins were highest in autumn.					
30242779	1	66	dep	production	220:229	arg1	both					201:204	both	201:204	both	201:204	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	4	67	theme	Chemical	484:491	arg1	composition					498:508	Chemical milk composition	484:508	Chemical milk composition	484:508	Chemical milk composition was determined by Lactoscan.					
30242779	9	68	theme	fat	963:965	arg1	lactose					996:1002	lactose	996:1002	lactose	996:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	%					958:958	7.08%	954:958	7.08% of fat	954:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	proteins					977:984	proteins	977:984	proteins	977:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	%					935:935	16.17%	930:935	16.17% of total solids	930:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	%					1038:1038	0.73%	1034:1038	0.73% of ash	1034:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	%					1009:1009	9.09%	1005:1009	9.09% of solids-not-fat	1005:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	fat					963:965	fat	963:965	fat	963:965	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	solids					946:951	total solids	940:951	total solids	940:951	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	solids-not-fat					1014:1027	solids-not-fat	1014:1027	solids-not-fat	1014:1027	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	ash					1043:1045	ash	1043:1045	ash	1043:1045	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	%					991:991	4.32%	987:991	4.32% of lactose	987:1002	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	9	68	theme	fat	963:965	arg1	%					972:972	4.04%	968:972	4.04% of proteins	968:984	Overall, milk contained 16.17% of total solids, 7.08% of fat, 4.04% of proteins, 4.32% of lactose, 9.09% of solids-not-fat, and 0.73% of ash.					
30242779	0	69	theme	D'	76:77	arg1	ewes					82:85	prolific D'man ewes	67:85	prolific D'man ewes	67:85	Factors affecting the milk yield and composition over lactation of prolific D'man ewes in Tunisian oases.					
30242779	17	70	theme	litter	1815:1820	arg1	survival					1822:1829	litter survival	1815:1829	litter survival at weaning	1815:1840	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	0	71	theme	ewes	82:85	arg1	lactation					54:62	lactation	54:62	lactation of prolific D'man ewes in Tunisian oases	54:103	Factors affecting the milk yield and composition over lactation of prolific D'man ewes in Tunisian oases.					
30242779	14	72	theme	Adult	1448:1452	arg1	ewes					1454:1457	Adult ewes	1448:1457	Adult ewes	1448:1457	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	8	73	theme	man	802:804	arg1	ewes					806:809	D'man ewes	800:809	D'man ewes	800:809	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	17	74	theme	proteins	1913:1920	arg1	fat					1898:1900	fat	1898:1900	fat	1898:1900	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	74	theme	proteins	1913:1920	arg1	amounts					1875:1881	the amounts	1871:1881	the amounts of total milk, fat, and total proteins	1871:1920	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	74	theme	proteins	1913:1920	arg1	milk					1892:1895	total milk	1886:1895	total milk	1886:1895	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	74	theme	proteins	1913:1920	arg1	proteins					1913:1920	total proteins	1907:1920	total proteins	1907:1920	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	0	75	theme	Tunisian	90:97	arg1	oases					99:103	Tunisian oases	90:103	Tunisian oases	90:103	Factors affecting the milk yield and composition over lactation of prolific D'man ewes in Tunisian oases.					
30242779	10	76	theme	total	1129:1133	arg1	proteins					1135:1142	fat and total proteins	1121:1142	fat and total proteins	1121:1142	Lambing season affected the daily milk yield and milk composition, where fat and total proteins were highest in autumn.					
30242779	2	77	theme	oases	361:365	arg1	system					367:372	an intensive oases system	348:372	an intensive oases system in Southern Tunisia	348:392	Observations were performed in eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia.					
30242779	3	78	theme	milk	401:404	arg1	yield					406:410	Daily milk yield	395:410	Daily milk yield	395:410	Daily milk yield was determined at 7-day intervals using oxytocin + hand milking method.					
30242779	0	79	theme	milk	22:25	arg1	yield					27:31	the milk yield	18:31	the milk yield	18:31	Factors affecting the milk yield and composition over lactation of prolific D'man ewes in Tunisian oases.					
30242779	1	80	theme	D'	259:260	arg1	sheep					265:269	prolific D'man sheep	250:269	prolific D'man sheep	250:269	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	15	81	with	ewes	1680:1683	arg1	udder					1702:1706	symmetrical udder	1690:1706	symmetrical udder	1690:1706	Ewes with asymmetric udder produced less and more concentrated milk than ewes with symmetrical udder.					
30242779	16	82	theme	total	1778:1782	arg1	amounts					1793:1799	total proteins amounts	1778:1799	total proteins amounts	1778:1799	Daily milk yield was negatively correlated to total solids, fat, and total proteins amounts.					
30242779	6	83	theme	milk	648:651	arg1	yield					653:657	Daily milk yield	642:657	Daily milk yield	642:657	Daily milk yield averaged 1.64 ± 0.02 l.					
30242779	12	84	theme	reared	1321:1326	arg1	singles					1328:1334	those reared singles	1315:1334	those reared singles	1315:1334	Ewes reared multiple lambs produced more milk than those reared singles.					
30242779	1	85	theme	milk	209:212	arg1	yield					214:218	milk yield	209:218	milk yield	209:218	This work aimed to investigate milk potentiality and the influence of some non-genetic factors both on milk yield production and composition in prolific D'man sheep.					
30242779	14	86	contain	contained	1529:1537	arg1	milk					1524:1527	their milk	1518:1527	their milk	1518:1527	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	14	86	contain	contained	1529:1537	arg2	fat					1560:1562	fat	1560:1562	fat	1560:1562	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	14	86	contain	contained	1529:1537	arg2	solids					1552:1557	higher total solids	1539:1557	higher total solids	1539:1557	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	14	86	contain	contained	1529:1537	arg2	levels					1573:1578	ash levels	1569:1578	ash levels	1569:1578	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	7	87	theme	lactation	746:754	arg1	week					738:741	the fourth week	727:741	the fourth week of lactation	727:754	It increased to a plateau from the third to the fourth week of lactation, and decreased gradually to the tenth week.					
30242779	16	88	theme	Daily	1709:1713	arg1	yield					1720:1724	Daily milk yield	1709:1724	Daily milk yield	1709:1724	Daily milk yield was negatively correlated to total solids, fat, and total proteins amounts.					
30242779	13	89	theme	fat	1436:1438	arg1	levels					1440:1445	fat levels	1436:1445	fat levels	1436:1445	Dams nursing triplets or more had the highest milk total proteins, and the lowest total solids and fat levels.					
30242779	17	90	theme	milk	1892:1895	arg1	fat					1898:1900	fat	1898:1900	fat	1898:1900	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	90	theme	milk	1892:1895	arg1	amounts					1875:1881	the amounts	1871:1881	the amounts of total milk, fat, and total proteins	1871:1920	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	90	theme	milk	1892:1895	arg1	milk					1892:1895	total milk	1886:1895	total milk	1886:1895	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	90	theme	milk	1892:1895	arg1	proteins					1913:1920	total proteins	1907:1920	total proteins	1907:1920	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	2	91	theme	lactating	310:318	arg1	ewes					326:329	eighty lactating D'man ewes	303:329	eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia	303:392	Observations were performed in eighty lactating D'man ewes maintained under an intensive oases system in Southern Tunisia.					
30242779	12	92	theme	more	1300:1303	arg1	milk					1305:1308	more milk	1300:1308	more milk	1300:1308	Ewes reared multiple lambs produced more milk than those reared singles.					
30242779	17	93	theme	fat	1898:1900	arg1	fat					1898:1900	fat	1898:1900	fat	1898:1900	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	93	theme	fat	1898:1900	arg1	amounts					1875:1881	the amounts	1871:1881	the amounts of total milk, fat, and total proteins	1871:1920	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	93	theme	fat	1898:1900	arg1	milk					1892:1895	total milk	1886:1895	total milk	1886:1895	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	93	theme	fat	1898:1900	arg1	proteins					1913:1920	total proteins	1907:1920	total proteins	1907:1920	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	17	94	from	weaning	1834:1840	arg1	survival					1822:1829	litter survival	1815:1829	litter survival at weaning	1815:1840	In contrast, litter survival at weaning was positively correlated to the amounts of total milk, fat, and total proteins.					
30242779	3	95	theme	oxytocin + hand	452:466	arg1	method					476:481	oxytocin + hand milking method	452:481	oxytocin + hand milking method	452:481	Daily milk yield was determined at 7-day intervals using oxytocin + hand milking method.					
30242779	8	96	theme	total	890:894	arg1	proteins					896:903	total proteins	890:903	total proteins	890:903	D'man ewes produced 128.91 ± 3.14 l of milk, 9.23 ± 0.26 kg of fat, and 5.26 ± 0.12 kg of total proteins.					
30242779	14	97	from	ewes	1601:1604	arg1	milk					1585:1588	milk	1585:1588	milk from young ewes	1585:1604	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30242779	14	98	theme	higher	1539:1544	arg1	solids					1552:1557	higher total solids	1539:1557	higher total solids	1539:1557	Adult ewes produced more milk compared to younger and older ewes, and their milk contained higher total solids, fat, and ash levels than milk from young ewes.					
30797808	1	0	dep	scaffold	256:263	arg1	%					268:268	92%	266:268	92% in porosity	266:280	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	1	1	from	%	268:268	arg1	porosity					273:280	porosity	273:280	porosity	273:280	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	1	2	theme	extracellular	156:168	arg1	matrix					170:175	tissue extracellular matrix	149:175	tissue extracellular matrix	149:175	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	1	3	theme	matrix	170:175	arg1	structure					136:144	the natural structure	124:144	the natural structure of tissue extracellular matrix	124:175	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	1	4	theme	/sodium	224:230	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	8	5	theme	wound	1327:1331	arg1	dressing					1333:1340	a wound dressing	1325:1340	a wound dressing for skin repair	1325:1356	Our findings showed that the prepared SF/HA/SA scaffold can provide a potential way as a wound dressing for skin repair.					
30797808	3	6	theme	soft	503:506	arg1	characteristics					517:531	soft, elastic characteristics	503:531	soft, elastic characteristics	503:531	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	1	7	theme	alginate	232:239	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	0	8	theme	skin	102:105	arg1	repair					107:112	skin repair	102:112	skin repair	102:112	Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair.					
30797808	7	9	theme	trichrome	1103:1111	arg1	staining					1113:1120	Masson's trichrome staining	1094:1120	Masson's trichrome staining	1094:1120	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling.					
30797808	4	10	theme	mechanical	729:738	arg1	environments					757:768	mechanical movement-related environments	729:768	mechanical movement-related environments	729:768	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	3	11	theme	electron	436:443	arg1	microscopy					445:454	Scanning electron microscopy	427:454	Scanning electron microscopy	427:454	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	4	12	theme	property	585:592	arg1	results					638:644	Mechanical property, thermogravimetric analyses and degradation results	574:644	Mechanical property, thermogravimetric analyses and degradation results	574:644	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	6	13	theme	rat	1031:1033	arg1	model					1055:1059	a rat full-thickness burn model	1029:1059	a rat full-thickness burn model	1029:1059	We evaluated the wound healing effects in a rat full-thickness burn model.					
30797808	2	14	theme	Raman	358:362	arg1	spectra					364:370	Raman spectra	358:370	Raman spectra	358:370	Fourier-transform infrared spectroscopy and Raman spectra indicated interactions among SF, HA, and SA molecules.					
30797808	5	15	theme	morphological	791:803	arg1	proliferation					776:788	Cell proliferation	771:788	Cell proliferation	771:788	Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds.					
30797808	4	16	contain	had	683:685	arg2	stability					701:709	good physical stability	687:709	good physical stability	687:709	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	4	16	contain	had	683:685	arg1	scaffold					674:681	the SF/HA/SA scaffold	661:681	the SF/HA/SA scaffold	661:681	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	5	17	theme	Cell	771:774	arg1	proliferation					776:788	Cell proliferation	771:788	Cell proliferation	771:788	Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds.					
30797808	6	18	theme	full-thickness	1035:1048	arg1	model					1055:1059	a rat full-thickness burn model	1029:1059	a rat full-thickness burn model	1029:1059	We evaluated the wound healing effects in a rat full-thickness burn model.					
30797808	7	19	theme	SF/HA/SA	1135:1142	arg1	scaffold					1144:1151	SF/HA/SA scaffold	1135:1151	SF/HA/SA scaffold	1135:1151	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling.					
30797808	7	20	from	scaffold	1144:1151	arg1	results					1122:1128	The hematoxylin-eosin (H&E) and Masson's trichrome staining results	1062:1128	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold	1062:1151	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling.					
30797808	6	21	theme	burn	1050:1053	arg1	model					1055:1059	a rat full-thickness burn model	1029:1059	a rat full-thickness burn model	1029:1059	We evaluated the wound healing effects in a rat full-thickness burn model.					
30797808	7	22	theme	extracellular	1205:1217	arg1	remodeling					1226:1235	extracellular matrix remodeling	1205:1235	extracellular matrix remodeling	1205:1235	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling.					
30797808	4	23	theme	body	714:717	arg1	fluid					719:723	body fluid	714:723	body fluid	714:723	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	1	24	theme	novel	180:184	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	2	25	theme	HA	405:406	arg1	molecules					416:424	SF, HA, and SA molecules	401:424	SF, HA, and SA molecules	401:424	Fourier-transform infrared spectroscopy and Raman spectra indicated interactions among SF, HA, and SA molecules.					
30797808	1	26	theme	SA	242:243	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	4	27	theme	physical	692:699	arg1	stability					701:709	good physical stability	687:709	good physical stability	687:709	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	5	28	theme	SF/HA/SA	923:930	arg1	scaffold					932:939	the SF/HA/SA scaffold	919:939	the SF/HA/SA scaffold	919:939	Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds.					
30797808	1	29	theme	silk	186:189	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	5	30	theme	NIH-3T3	841:847	arg1	cells					860:864	NIH-3T3 fibroblast cells	841:864	NIH-3T3 fibroblast cells	841:864	Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds.					
30797808	6	31	theme	healing	1010:1016	arg1	effects					1018:1024	the wound healing effects	1000:1024	the wound healing effects	1000:1024	We evaluated the wound healing effects in a rat full-thickness burn model.					
30797808	7	32	theme	hematoxylin-eosin	1066:1082	arg1	results					1122:1128	The hematoxylin-eosin (H&E) and Masson's trichrome staining results	1062:1128	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold	1062:1151	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling.					
30797808	1	33	theme	fibroin	191:197	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	2	34	theme	SF	401:402	arg1	molecules					416:424	SF, HA, and SA molecules	401:424	SF, HA, and SA molecules	401:424	Fourier-transform infrared spectroscopy and Raman spectra indicated interactions among SF, HA, and SA molecules.					
30797808	1	35	theme	composite	246:254	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	3	36	dep	soft	503:506	arg1	elastic					509:515	elastic	509:515	elastic	509:515	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	4	37	theme	SF/HA/SA	665:672	arg1	scaffold					674:681	the SF/HA/SA scaffold	661:681	the SF/HA/SA scaffold	661:681	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	7	38	theme	staining	1113:1120	arg1	results					1122:1128	The hematoxylin-eosin (H&E) and Masson's trichrome staining results	1062:1128	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold	1062:1151	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling.					
30797808	3	39	theme	prepared	472:479	arg1	scaffold					490:497	the prepared SF/HA/SA scaffold	468:497	the prepared SF/HA/SA scaffold	468:497	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	0	40	theme	novel	38:42	arg1	silk					44:47	a novel silk	36:47	a novel silk	36:47	Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair.					
30797808	4	41	theme	analyses	613:620	arg1	results					638:644	Mechanical property, thermogravimetric analyses and degradation results	574:644	Mechanical property, thermogravimetric analyses and degradation results	574:644	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	3	42	theme	pore	550:553	arg1	diameter					555:562	an average pore diameter	539:562	an average pore diameter of 93 μm	539:571	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	2	43	theme	infrared	332:339	arg1	spectroscopy					341:352	Fourier-transform infrared spectroscopy	314:352	Fourier-transform infrared spectroscopy	314:352	Fourier-transform infrared spectroscopy and Raman spectra indicated interactions among SF, HA, and SA molecules.					
30797808	1	44	theme	SF	200:201	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	4	45	theme	degradation	626:636	arg1	results					638:644	Mechanical property, thermogravimetric analyses and degradation results	574:644	Mechanical property, thermogravimetric analyses and degradation results	574:644	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	3	46	contain	had	499:501	arg1	scaffold					490:497	the prepared SF/HA/SA scaffold	468:497	the prepared SF/HA/SA scaffold	468:497	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	3	46	contain	had	499:501	arg2	characteristics					517:531	soft, elastic characteristics	503:531	soft, elastic characteristics	503:531	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	5	47	theme	proliferation	776:788	arg1	analyses					820:827	Cell proliferation, morphological, and live-dead analyses	771:827	Cell proliferation, morphological, and live-dead analyses	771:827	Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds.					
30797808	8	48	theme	prepared	1267:1274	arg1	scaffold					1285:1292	the prepared SF/HA/SA scaffold	1263:1292	the prepared SF/HA/SA scaffold	1263:1292	Our findings showed that the prepared SF/HA/SA scaffold can provide a potential way as a wound dressing for skin repair.					
30797808	5	49	theme	live-dead	810:818	arg1	analyses					820:827	Cell proliferation, morphological, and live-dead analyses	771:827	Cell proliferation, morphological, and live-dead analyses	771:827	Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds.					
30797808	4	50	theme	thermogravimetric	595:611	arg1	analyses					613:620	thermogravimetric analyses	595:620	thermogravimetric analyses	595:620	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	2	51	theme	SA	413:414	arg1	molecules					416:424	SF, HA, and SA molecules	401:424	SF, HA, and SA molecules	401:424	Fourier-transform infrared spectroscopy and Raman spectra indicated interactions among SF, HA, and SA molecules.					
30797808	6	52	theme	wound	1004:1008	arg1	healing					1010:1016	wound healing	1004:1016	the wound healing effects	1000:1024	We evaluated the wound healing effects in a rat full-thickness burn model.					
30797808	7	53	theme	matrix	1219:1224	arg1	remodeling					1226:1235	extracellular matrix remodeling	1205:1235	extracellular matrix remodeling	1205:1235	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling.					
30797808	8	54	theme	skin	1346:1349	arg1	repair					1351:1356	skin repair	1346:1356	skin repair	1346:1356	Our findings showed that the prepared SF/HA/SA scaffold can provide a potential way as a wound dressing for skin repair.					
30797808	4	55	theme	good	687:690	arg1	stability					701:709	good physical stability	687:709	good physical stability	687:709	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	0	56	theme	silk	44:47	arg1	characterisation					16:31	characterisation	16:31	characterisation	16:31	Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair.					
30797808	0	56	theme	silk	44:47	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair.					
30797808	3	57	theme	Scanning	427:434	arg1	microscopy					445:454	Scanning electron microscopy	427:454	Scanning electron microscopy	427:454	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	3	58	theme	average	542:548	arg1	diameter					555:562	an average pore diameter	539:562	an average pore diameter of 93 μm	539:571	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	8	59	theme	SF/HA/SA	1276:1283	arg1	scaffold					1285:1292	the prepared SF/HA/SA scaffold	1263:1292	the prepared SF/HA/SA scaffold	1263:1292	Our findings showed that the prepared SF/HA/SA scaffold can provide a potential way as a wound dressing for skin repair.					
30797808	8	60	theme	potential	1308:1316	arg1	way					1318:1320	a potential way	1306:1320	a potential way as a wound dressing for skin repair	1306:1356	Our findings showed that the prepared SF/HA/SA scaffold can provide a potential way as a wound dressing for skin repair.					
30797808	7	61	dep	improved	1165:1172	arg1	enhanced					1196:1203	enhanced	1196:1203	enhanced extracellular matrix remodeling	1196:1235	The hematoxylin-eosin (H&E) and Masson's trichrome staining results from SF/HA/SA scaffold showed that improved re-epithelialization, enhanced extracellular matrix remodeling.					
30797808	1	62	theme	natural	128:134	arg1	structure					136:144	the natural structure	124:144	the natural structure of tissue extracellular matrix	124:175	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	1	63	theme	/hyaluronic	203:213	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	4	64	theme	movement-related	740:755	arg1	environments					757:768	mechanical movement-related environments	729:768	mechanical movement-related environments	729:768	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	3	65	theme	SF/HA/SA	481:488	arg1	scaffold					490:497	the prepared SF/HA/SA scaffold	468:497	the prepared SF/HA/SA scaffold	468:497	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	1	66	theme	acid	215:218	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	0	67	theme	alginate	80:87	arg1	scaffold					89:96	acid/sodium alginate scaffold	68:96	acid/sodium alginate scaffold for skin repair	68:112	Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair.					
30797808	5	68	theme	fibroblast	849:858	arg1	cells					860:864	NIH-3T3 fibroblast cells	841:864	NIH-3T3 fibroblast cells	841:864	Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds.					
30797808	5	69	theme	SF/SA	970:974	arg1	scaffolds					976:984	SF/SA scaffolds	970:984	SF/SA scaffolds	970:984	Cell proliferation, morphological, and live-dead analyses showed that NIH-3T3 fibroblast cells were better able to attach, grow, and proliferate on the SF/HA/SA scaffold compared with SF, SF/HA, and SF/SA scaffolds.					
30797808	2	70	theme	Fourier-transform	314:330	arg1	spectroscopy					341:352	Fourier-transform infrared spectroscopy	314:352	Fourier-transform infrared spectroscopy	314:352	Fourier-transform infrared spectroscopy and Raman spectra indicated interactions among SF, HA, and SA molecules.					
30797808	4	71	theme	Mechanical	574:583	arg1	property					585:592	Mechanical property	574:592	Mechanical property	574:592	Mechanical property, thermogravimetric analyses and degradation results indicated that the SF/HA/SA scaffold had good physical stability in body fluid and mechanical movement-related environments.					
30797808	0	72	theme	acid/sodium	68:78	arg1	scaffold					89:96	acid/sodium alginate scaffold	68:96	acid/sodium alginate scaffold for skin repair	68:112	Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair.					
30797808	3	73	theme	93 μm	567:571	arg1	diameter					555:562	an average pore diameter	539:562	an average pore diameter of 93 μm	539:571	Scanning electron microscopy showed that the prepared SF/HA/SA scaffold had soft, elastic characteristics, with an average pore diameter of 93 μm.					
30797808	1	74	theme	tissue	149:154	arg1	matrix					170:175	tissue extracellular matrix	149:175	tissue extracellular matrix	149:175	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
30797808	1	75	theme	HA	221:222	arg1	scaffold					256:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold	178:263	a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity)	178:281	To mimic the natural structure of tissue extracellular matrix, a novel silk fibroin (SF)/hyaluronic acid (HA)/sodium alginate (SA) composite scaffold (92% in porosity) was prepared by freeze-drying.					
29801858	2	0	theme	various	500:506	arg1	measurements					508:519	various measurements	500:519	various measurements	500:519	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films was investigated by various measurements.					
29801858	1	1	theme	cross-linking	343:355	arg1	process					357:363	a simultaneous casting and wet cross-linking process	312:363	process	357:363	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	4	2	from	delamination	790:801	arg1	water					819:823	water	819:823	water	819:823	Cross-linking improved the transparency, reduced the porosity and surface free energy, and prevented the delamination of CNC films in water at a concentration of 10% or higher.					
29801858	5	3	theme	CNC	877:879	arg1	films					881:885	CNC films	877:885	CNC films	877:885	The surface of CNC films is basic in character and has an electron donor property.					
29801858	1	4	theme	cross-linking	261:273	arg1	agent					275:279	an amino-aldehyde based cross-linking agent	237:279	an amino-aldehyde based cross-linking agent in a wide composition range	237:307	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	1	5	theme	transparent	162:172	arg1	films					185:189	transparent and smooth films	162:189	transparent and smooth films	162:189	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	4	6	theme	CNC	806:808	arg1	films					810:814	CNC films	806:814	CNC films	806:814	Cross-linking improved the transparency, reduced the porosity and surface free energy, and prevented the delamination of CNC films in water at a concentration of 10% or higher.					
29801858	5	7	theme	donor	929:933	arg1	property					935:942	an electron donor property	917:942	an electron donor property	917:942	The surface of CNC films is basic in character and has an electron donor property.					
29801858	5	8	theme	electron	920:927	arg1	property					935:942	an electron donor property	917:942	an electron donor property	917:942	The surface of CNC films is basic in character and has an electron donor property.					
29801858	3	9	with	films	541:545	arg1	water					559:563	liquid water	552:563	liquid water	552:563	The interaction of films with liquid water and water vapour was also characterized by water sorption and water contact angle as well as performing a sinking test.					
29801858	3	9	with	films	541:545	arg1	vapour					575:580	water vapour	569:580	water vapour	569:580	The interaction of films with liquid water and water vapour was also characterized by water sorption and water contact angle as well as performing a sinking test.					
29801858	1	10	theme	cellulose	81:89	arg1	nanocrystals					91:102	cellulose nanocrystals	81:102	cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis	81:159	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	1	10	theme	cellulose	81:89	arg1	CNCs					105:108	CNCs	105:108	CNCs	105:108	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	6	11	theme	high	980:983	arg1	45 MPa					1003:1008	45 MPa	1003:1008	45 MPa	1003:1008	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	6	11	theme	high	980:983	arg1	strength					993:1000	a high tensile strength	978:1000	a high tensile strength (45 MPa)	978:1009	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	1	12	theme	nanocrystals	91:102	arg1	suspensions					66:76	the suspensions	62:76	the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis	62:159	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	2	13	theme	optical	414:420	arg1	properties					434:443	the optical and tensile properties	410:443	the optical and tensile properties	410:443	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films was investigated by various measurements.					
29801858	6	14	theme	CNC/amino-aldehyde	949:966	arg1	films					968:972	The CNC/amino-aldehyde films	945:972	The CNC/amino-aldehyde films	945:972	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	5	15	theme	films	881:885	arg1	basic					890:894	basic	890:894	basic	890:894	The surface of CNC films is basic in character and has an electron donor property.					
29801858	5	15	theme	films	881:885	arg1	surface					866:872	The surface	862:872	The surface of CNC films	862:885	The surface of CNC films is basic in character and has an electron donor property.					
29801858	2	16	from	effect	370:375	arg1	morphology					456:465	the morphology	452:465	the morphology of CNC films	452:478	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films was investigated by various measurements.					
29801858	2	16	from	effect	370:375	arg1	properties					434:443	the optical and tensile properties	410:443	the optical and tensile properties	410:443	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films was investigated by various measurements.					
29801858	3	17	theme	liquid	552:557	arg1	water					559:563	liquid water	552:563	liquid water	552:563	The interaction of films with liquid water and water vapour was also characterized by water sorption and water contact angle as well as performing a sinking test.					
29801858	1	18	theme	smooth	178:183	arg1	films					185:189	transparent and smooth films	162:189	transparent and smooth films	162:189	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	4	19	theme	%	849:849	arg1	concentration					830:842	a concentration	828:842	a concentration of 10% or higher	828:859	Cross-linking improved the transparency, reduced the porosity and surface free energy, and prevented the delamination of CNC films in water at a concentration of 10% or higher.					
29801858	5	20	from	character	899:907	arg1	basic					890:894	basic	890:894	basic	890:894	The surface of CNC films is basic in character and has an electron donor property.					
29801858	5	20	from	character	899:907	arg1	surface					866:872	The surface	862:872	The surface of CNC films	862:885	The surface of CNC films is basic in character and has an electron donor property.					
29801858	4	21	theme	surface	751:757	arg1	energy					764:769	surface free energy	751:769	surface free energy	751:769	Cross-linking improved the transparency, reduced the porosity and surface free energy, and prevented the delamination of CNC films in water at a concentration of 10% or higher.					
29801858	1	22	theme	wide	286:289	arg1	range					303:307	a wide composition range	284:307	a wide composition range	284:307	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	2	23	theme	concentration	393:405	arg1	effect					370:375	The effect	366:375	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films	366:478	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films was investigated by various measurements.					
29801858	1	24	theme	composition	291:301	arg1	range					303:307	a wide composition range	284:307	a wide composition range	284:307	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	5	25	from	basic	890:894	arg1	character					899:907	character	899:907	character	899:907	The surface of CNC films is basic in character and has an electron donor property.					
29801858	3	26	theme	water	569:573	arg1	vapour					575:580	water vapour	569:580	water vapour	569:580	The interaction of films with liquid water and water vapour was also characterized by water sorption and water contact angle as well as performing a sinking test.					
29801858	0	27	theme	biocomposite	37:48	arg1	films					50:54	biocomposite films	37:54	biocomposite films	37:54	Cellulose nanocrystal/amino-aldehyde biocomposite films.					
29801858	4	28	theme	free	759:762	arg1	energy					764:769	surface free energy	751:769	surface free energy	751:769	Cross-linking improved the transparency, reduced the porosity and surface free energy, and prevented the delamination of CNC films in water at a concentration of 10% or higher.					
29801858	5	29	contain	has	913:915	arg1	basic					890:894	basic	890:894	basic	890:894	The surface of CNC films is basic in character and has an electron donor property.					
29801858	5	29	contain	has	913:915	arg1	surface					866:872	The surface	862:872	The surface of CNC films	862:885	The surface of CNC films is basic in character and has an electron donor property.					
29801858	5	29	contain	has	913:915	arg2	property					935:942	an electron donor property	917:942	an electron donor property	917:942	The surface of CNC films is basic in character and has an electron donor property.					
29801858	1	30	theme	acidic	143:148	arg1	hydrolysis					150:159	acidic hydrolysis	143:159	acidic hydrolysis	143:159	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	2	31	theme	tensile	426:432	arg1	properties					434:443	the optical and tensile properties	410:443	the optical and tensile properties	410:443	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films was investigated by various measurements.					
29801858	3	32	theme	water	608:612	arg1	sorption					614:621	water sorption	608:621	water sorption	608:621	The interaction of films with liquid water and water vapour was also characterized by water sorption and water contact angle as well as performing a sinking test.					
29801858	1	33	theme	different	210:218	arg1	plasticizers					220:231	different plasticizers	210:231	different plasticizers	210:231	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	1	34	from	agent	275:279	arg1	range					303:307	a wide composition range	284:307	a wide composition range	284:307	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	6	35	theme	tensile	985:991	arg1	45 MPa					1003:1008	45 MPa	1003:1008	45 MPa	1003:1008	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	6	35	theme	tensile	985:991	arg1	strength					993:1000	a high tensile strength	978:1000	a high tensile strength (45 MPa)	978:1009	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	2	36	theme	films	474:478	arg1	morphology					456:465	the morphology	452:465	the morphology of CNC films	452:478	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films was investigated by various measurements.					
29801858	1	37	theme	simultaneous	314:325	arg1	casting					327:333	a simultaneous casting and wet cross-linking process	312:363	casting	327:333	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	1	38	from	plasticizers	220:231	arg1	range					303:307	a wide composition range	284:307	a wide composition range	284:307	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	2	39	theme	CNC	470:472	arg1	films					474:478	CNC films	470:478	CNC films	470:478	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films was investigated by various measurements.					
29801858	1	40	attach	derived	111:117	arg1	flax					135:138	flax	135:138	flax	135:138	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	1	40	attach	derived	111:117	arg1	cotton					124:129	cotton	124:129	cotton	124:129	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	1	40	attach	derived	111:117	arg2	CNCs					105:108	CNCs	105:108	CNCs	105:108	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	1	40	attach	derived	111:117	arg2	nanocrystals					91:102	cellulose nanocrystals	81:102	cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis	81:159	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	3	41	theme	contact	633:639	arg1	angle					641:645	water contact angle	627:645	water contact angle	627:645	The interaction of films with liquid water and water vapour was also characterized by water sorption and water contact angle as well as performing a sinking test.					
29801858	6	42	contain	had	974:976	arg2	45 MPa					1003:1008	45 MPa	1003:1008	45 MPa	1003:1008	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	6	42	contain	had	974:976	arg2	11 GPa					1024:1029	11 GPa	1024:1029	11 GPa	1024:1029	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	6	42	contain	had	974:976	arg2	strength					993:1000	a high tensile strength	978:1000	a high tensile strength (45 MPa)	978:1009	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	6	42	contain	had	974:976	arg2	modulus					1015:1021	modulus	1015:1021	modulus (11 GPa)	1015:1030	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	6	42	contain	had	974:976	arg1	films					968:972	The CNC/amino-aldehyde films	945:972	The CNC/amino-aldehyde films	945:972	The CNC/amino-aldehyde films had a high tensile strength (45 MPa) and modulus (11 GPa).					
29801858	3	43	theme	sinking	671:677	arg1	test					679:682	a sinking test	669:682	a sinking test	669:682	The interaction of films with liquid water and water vapour was also characterized by water sorption and water contact angle as well as performing a sinking test.					
29801858	4	44	theme	films	810:814	arg1	delamination					790:801	the delamination	786:801	the delamination of CNC films in water	786:823	Cross-linking improved the transparency, reduced the porosity and surface free energy, and prevented the delamination of CNC films in water at a concentration of 10% or higher.					
29801858	2	45	theme	cross-linker	380:391	arg1	concentration					393:405	cross-linker concentration	380:405	cross-linker concentration	380:405	The effect of cross-linker concentration on the optical and tensile properties and on the morphology of CNC films was investigated by various measurements.					
29801858	1	46	theme	amino-aldehyde	240:253	arg1	agent					275:279	an amino-aldehyde based cross-linking agent	237:279	an amino-aldehyde based cross-linking agent in a wide composition range	237:307	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	1	47	theme	wet	339:341	arg1	process					357:363	a simultaneous casting and wet cross-linking process	312:363	process	357:363	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
29801858	3	48	theme	water	627:631	arg1	angle					641:645	water contact angle	627:645	water contact angle	627:645	The interaction of films with liquid water and water vapour was also characterized by water sorption and water contact angle as well as performing a sinking test.					
29801858	3	49	theme	films	541:545	arg1	interaction					526:536	The interaction	522:536	The interaction of films with liquid water and water vapour	522:580	The interaction of films with liquid water and water vapour was also characterized by water sorption and water contact angle as well as performing a sinking test.					
29801858	1	50	theme	based	255:259	arg1	agent					275:279	an amino-aldehyde based cross-linking agent	237:279	an amino-aldehyde based cross-linking agent in a wide composition range	237:307	From the suspensions of cellulose nanocrystals (CNCs) derived from cotton and flax by acidic hydrolysis, transparent and smooth films were produced with different plasticizers and an amino-aldehyde based cross-linking agent in a wide composition range by a simultaneous casting and wet cross-linking process.					
31790736	7	0	theme	RBR	1206:1208	arg1	ions					1210:1213	RBR ions	1206:1213	RBR ions in solution	1206:1225	XPS analysis confirmed a potential adsorption mechanism that the N atoms on composite chelated with RBR ions in solution.					
31790736	3	1	theme	initial	454:460	arg1	concentration					466:478	initial dye concentration	454:478	initial dye concentration	454:478	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	1	2	theme	dye	238:240	arg1	RBR					242:244	anionic dye RBR	230:244	anionic dye RBR	230:244	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	4	3	theme	SnO2	804:807	arg1	introduction					788:799	the introduction	784:799	the introduction of SnO2	784:807	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	4	3	theme	SnO2	804:807	arg1	beneficial					813:822	beneficial	813:822	beneficial	813:822	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	7	4	from	ions	1210:1213	arg1	solution					1218:1225	solution	1218:1225	solution	1218:1225	XPS analysis confirmed a potential adsorption mechanism that the N atoms on composite chelated with RBR ions in solution.					
31790736	4	5	theme	CTS	612:614	arg1	Fe3O4					616:620	CTS@Fe3O4	612:620	CTS@Fe3O4	612:620	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	3	6	theme	temperature	484:494	arg1	Effects					400:406	Effects	400:406	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR)	400:544	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	1	7	theme	@	160:160	arg1	Fe3O4					166:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	9	8	theme	SnO2	1381:1384	arg1	Fe3O4					1386:1390	CTS@SnO2@Fe3O4	1377:1390	CTS@SnO2@Fe3O4	1377:1390	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	9	8	theme	SnO2	1381:1384	arg1	adsorbent					1436:1444	adsorbent	1436:1444	adsorbent	1436:1444	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	0	9	theme	dye	99:101	arg1	wastewater					103:112	dye wastewater	99:112	dye wastewater	99:112	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	2	10	theme	physicochemical	271:285	arg1	properties					287:296	The physicochemical properties	267:296	The physicochemical properties of the obtained material	267:321	The physicochemical properties of the obtained material were characterized by FTIR, XRD, VSM, TGA, SEM and N2 adsorption-desorption.					
31790736	4	11	theme	@	631:631	arg1	Fe3O4					632:636	CTS@SnO2@Fe3O4	623:636	CTS@SnO2@Fe3O4	623:636	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	8	12	theme	@	1244:1244	arg1	particles					1256:1264	CTS@SnO2@Fe3O4 particles	1241:1264	CTS@SnO2@Fe3O4 particles	1241:1264	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	8	13	theme	@	1249:1249	arg1	particles					1256:1264	CTS@SnO2@Fe3O4 particles	1241:1264	CTS@SnO2@Fe3O4 particles	1241:1264	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	9	14	theme	higher	1477:1482	arg1	capacity					1495:1502	its higher adsorption capacity	1473:1502	its higher adsorption capacity compared with other adsorbents	1473:1533	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	3	15	theme	batch	568:572	arg1	experiments					585:595	batch adsorption experiments	568:595	batch adsorption experiments	568:595	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	4	16	theme	better	645:650	arg1	performance					663:673	better adsorption performance	645:673	better adsorption performance	645:673	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	9	17	dep	All	1365:1367	arg1	all					1372:1374	all	1372:1374	all	1372:1374	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	4	18	theme	CTS	623:625	arg1	Fe3O4					632:636	CTS@SnO2@Fe3O4	623:636	CTS@SnO2@Fe3O4	623:636	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	0	19	theme	@	60:60	arg1	Fe3O4					66:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	1	20	theme	chitosan-based	123:136	arg1	Fe3O4					166:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	4	21	theme	SnO2	627:630	arg1	Fe3O4					632:636	CTS@SnO2@Fe3O4	623:636	CTS@SnO2@Fe3O4	623:636	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	0	22	theme	@	65:65	arg1	Fe3O4					66:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	1	23	theme	composite	147:155	arg1	Fe3O4					166:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	9	24	theme	dye	1454:1456	arg1	removal					1458:1464	the dye removal	1450:1464	the dye removal due to its higher adsorption capacity compared with other adsorbents	1450:1533	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	8	25	from	easy	1271:1274	arg1	addition					1231:1238	addition	1231:1238	addition	1231:1238	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	9	26	theme	due	1466:1468	arg1	removal					1458:1464	the dye removal	1450:1464	the dye removal due to its higher adsorption capacity compared with other adsorbents	1450:1533	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	6	27	theme	parameters	1025:1034	arg1	Evaluation					993:1002	Evaluation	993:1002	Evaluation of the thermodynamic parameters	993:1034	Evaluation of the thermodynamic parameters revealed that the adsorption process was spontaneous and endothermic.					
31790736	1	28	theme	aqueous	249:255	arg1	solution					257:264	aqueous solution	249:264	aqueous solution	249:264	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	5	29	theme	equilibrium	861:871	arg1	behaviors					884:892	equilibrium adsorption behaviors	861:892	equilibrium adsorption behaviors	861:892	The kinetic data and equilibrium adsorption behaviors were well depicted by pesudo-second-order kinetic model and Langmuir isotherm model, respectively.					
31790736	2	30	theme	N2	374:375	arg1	adsorption-desorption					377:397	N2 adsorption-desorption	374:397	N2 adsorption-desorption	374:397	The physicochemical properties of the obtained material were characterized by FTIR, XRD, VSM, TGA, SEM and N2 adsorption-desorption.					
31790736	7	31	theme	potential	1131:1139	arg1	mechanism					1152:1160	a potential adsorption mechanism	1129:1160	a potential adsorption mechanism that the N atoms on composite chelated with RBR ions in solution	1129:1225	XPS analysis confirmed a potential adsorption mechanism that the N atoms on composite chelated with RBR ions in solution.					
31790736	9	32	theme	adsorption	1484:1493	arg1	capacity					1495:1502	its higher adsorption capacity	1473:1502	its higher adsorption capacity compared with other adsorbents	1473:1533	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	3	33	theme	contact	411:417	arg1	time					419:422	contact time	411:422	contact time	411:422	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	0	34	theme	chitosan-based	23:36	arg1	Fe3O4					66:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	5	35	theme	isotherm	963:970	arg1	model					972:976	Langmuir isotherm model	954:976	Langmuir isotherm model	954:976	The kinetic data and equilibrium adsorption behaviors were well depicted by pesudo-second-order kinetic model and Langmuir isotherm model, respectively.					
31790736	8	36	theme	recycling	1354:1362	arg1	times					1348:1352	five times recycling	1343:1362	five times recycling	1343:1362	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	3	37	theme	ionic	438:442	arg1	strength					444:451	ionic strength	438:451	ionic strength	438:451	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	7	38	theme	XPS	1106:1108	arg1	analysis					1110:1117	XPS analysis	1106:1117	XPS analysis	1106:1117	XPS analysis confirmed a potential adsorption mechanism that the N atoms on composite chelated with RBR ions in solution.					
31790736	9	39	theme	other	1518:1522	arg1	adsorbents					1524:1533	other adsorbents	1518:1533	other adsorbents	1518:1533	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	2	40	theme	material	314:321	arg1	properties					287:296	The physicochemical properties	267:296	The physicochemical properties of the obtained material	267:321	The physicochemical properties of the obtained material were characterized by FTIR, XRD, VSM, TGA, SEM and N2 adsorption-desorption.					
31790736	1	41	theme	water-in-oil	188:199	arg1	emulsification					201:214	water-in-oil emulsification	188:214	water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution	188:264	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	5	42	theme	kinetic	936:942	arg1	model					944:948	pesudo-second-order kinetic model	916:948	pesudo-second-order kinetic model	916:948	The kinetic data and equilibrium adsorption behaviors were well depicted by pesudo-second-order kinetic model and Langmuir isotherm model, respectively.					
31790736	3	43	theme	dye	462:464	arg1	concentration					466:478	initial dye concentration	454:478	initial dye concentration	454:478	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	9	44	theme	CTS	1377:1379	arg1	Fe3O4					1386:1390	CTS@SnO2@Fe3O4	1377:1390	CTS@SnO2@Fe3O4	1377:1390	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	9	44	theme	CTS	1377:1379	arg1	adsorbent					1436:1444	adsorbent	1436:1444	adsorbent	1436:1444	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	6	45	theme	adsorption	1054:1063	arg1	spontaneous					1077:1087	spontaneous	1077:1087	spontaneous	1077:1087	Evaluation of the thermodynamic parameters revealed that the adsorption process was spontaneous and endothermic.					
31790736	6	45	theme	adsorption	1054:1063	arg1	process					1065:1071	the adsorption process	1050:1071	the adsorption process	1050:1071	Evaluation of the thermodynamic parameters revealed that the adsorption process was spontaneous and endothermic.					
31790736	1	46	theme	anionic	230:236	arg1	RBR					242:244	anionic dye RBR	230:244	anionic dye RBR	230:244	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	9	47	theme	@	1380:1380	arg1	Fe3O4					1386:1390	CTS@SnO2@Fe3O4	1377:1390	CTS@SnO2@Fe3O4	1377:1390	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	9	47	theme	@	1380:1380	arg1	adsorbent					1436:1444	adsorbent	1436:1444	adsorbent	1436:1444	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	1	48	theme	CTS	157:159	arg1	Fe3O4					166:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	0	49	theme	adsorbent	47:55	arg1	Fe3O4					66:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	1	50	theme	SnO2	161:164	arg1	Fe3O4					166:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	4	51	theme	@	615:615	arg1	Fe3O4					616:620	CTS@Fe3O4	612:620	CTS@Fe3O4	612:620	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	0	52	theme	wastewater	103:112	arg1	treatment					86:94	effective treatment	76:94	effective treatment of dye wastewater	76:112	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	9	53	theme	@	1385:1385	arg1	Fe3O4					1386:1390	CTS@SnO2@Fe3O4	1377:1390	CTS@SnO2@Fe3O4	1377:1390	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	9	53	theme	@	1385:1385	arg1	adsorbent					1436:1444	adsorbent	1436:1444	adsorbent	1436:1444	All in all, CTS@SnO2@Fe3O4 was proven to be an efficient and promising adsorbent for the dye removal due to its higher adsorption capacity compared with other adsorbents.					
31790736	1	54	theme	@	165:165	arg1	Fe3O4					166:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	3	55	theme	red	536:538	arg1	adsorption					503:512	the adsorption	499:512	the adsorption of reactive brilliant red (RBR)	499:544	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	8	56	theme	CTS	1241:1243	arg1	particles					1256:1264	CTS@SnO2@Fe3O4 particles	1241:1264	CTS@SnO2@Fe3O4 particles	1241:1264	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	5	57	theme	kinetic	844:850	arg1	data					852:855	The kinetic data	840:855	The kinetic data	840:855	The kinetic data and equilibrium adsorption behaviors were well depicted by pesudo-second-order kinetic model and Langmuir isotherm model, respectively.					
31790736	8	58	theme	SnO2	1245:1248	arg1	particles					1256:1264	CTS@SnO2@Fe3O4 particles	1241:1264	CTS@SnO2@Fe3O4 particles	1241:1264	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	3	59	theme	pH	434:435	arg1	Effects					400:406	Effects	400:406	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR)	400:544	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	0	60	theme	SnO2	61:64	arg1	Fe3O4					66:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	8	61	from	addition	1231:1238	arg1	easy					1271:1274	easy	1271:1274	easy	1271:1274	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	1	62	theme	novel	117:121	arg1	Fe3O4					166:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	8	63	theme	Fe3O4	1250:1254	arg1	particles					1256:1264	CTS@SnO2@Fe3O4 particles	1241:1264	CTS@SnO2@Fe3O4 particles	1241:1264	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	0	64	theme	Fe3O4	66:70	arg1	Preparation					0:10	Preparation	0:10	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.	0:113	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	1	65	theme	magnetic	138:145	arg1	Fe3O4					166:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4	115:170	A novel chitosan-based magnetic composite CTS@SnO2@Fe3O4 was prepared by water-in-oil emulsification for adsorbing anionic dye RBR in aqueous solution.					
31790736	8	66	contain	had	1309:1311	arg2	reusability					1325:1335	outstanding reusability	1313:1335	outstanding reusability	1313:1335	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	8	66	contain	had	1309:1311	arg1	particles					1256:1264	CTS@SnO2@Fe3O4 particles	1241:1264	CTS@SnO2@Fe3O4 particles	1241:1264	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	4	67	theme	@	626:626	arg1	Fe3O4					632:636	CTS@SnO2@Fe3O4	623:636	CTS@SnO2@Fe3O4	623:636	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	0	68	theme	effective	76:84	arg1	treatment					86:94	effective treatment	76:94	effective treatment of dye wastewater	76:112	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	7	69	theme	adsorption	1141:1150	arg1	mechanism					1152:1160	a potential adsorption mechanism	1129:1160	a potential adsorption mechanism that the N atoms on composite chelated with RBR ions in solution	1129:1225	XPS analysis confirmed a potential adsorption mechanism that the N atoms on composite chelated with RBR ions in solution.					
31790736	4	70	from	pH 2	760:763	arg1	maximum					734:740	a maximum	732:740	a maximum of 981.23 mg/g at pH 2	732:763	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	6	71	theme	thermodynamic	1011:1023	arg1	parameters					1025:1034	the thermodynamic parameters	1007:1034	the thermodynamic parameters	1007:1034	Evaluation of the thermodynamic parameters revealed that the adsorption process was spontaneous and endothermic.					
31790736	4	72	theme	adsorption	652:661	arg1	performance					663:673	better adsorption performance	645:673	better adsorption performance	645:673	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	0	73	theme	novel	17:21	arg1	Fe3O4					66:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	5	74	theme	adsorption	873:882	arg1	behaviors					884:892	equilibrium adsorption behaviors	861:892	equilibrium adsorption behaviors	861:892	The kinetic data and equilibrium adsorption behaviors were well depicted by pesudo-second-order kinetic model and Langmuir isotherm model, respectively.					
31790736	8	75	theme	outstanding	1313:1323	arg1	reusability					1325:1335	outstanding reusability	1313:1335	outstanding reusability	1313:1335	In addition, CTS@SnO2@Fe3O4 particles were easy to be magnetically separated and had outstanding reusability after five times recycling.					
31790736	3	76	theme	solution	425:432	arg1	pH					434:435	solution pH	425:435	solution pH	425:435	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	0	77	theme	magnetic	38:45	arg1	Fe3O4					66:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	5	78	theme	Langmuir	954:961	arg1	model					972:976	Langmuir isotherm model	954:976	Langmuir isotherm model	954:976	The kinetic data and equilibrium adsorption behaviors were well depicted by pesudo-second-order kinetic model and Langmuir isotherm model, respectively.					
31790736	3	79	theme	adsorption	574:583	arg1	experiments					585:595	batch adsorption experiments	568:595	batch adsorption experiments	568:595	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	3	80	theme	time	419:422	arg1	Effects					400:406	Effects	400:406	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR)	400:544	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	0	81	theme	CTS	57:59	arg1	Fe3O4					66:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4	15:70	Preparation of a novel chitosan-based magnetic adsorbent CTS@SnO2@Fe3O4 for effective treatment of dye wastewater.					
31790736	4	82	theme	981.23 mg/g	745:755	arg1	maximum					734:740	a maximum	732:740	a maximum of 981.23 mg/g at pH 2	732:763	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	3	83	theme	strength	444:451	arg1	Effects					400:406	Effects	400:406	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR)	400:544	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	5	84	theme	pesudo-second-order	916:934	arg1	model					944:948	pesudo-second-order kinetic model	916:948	pesudo-second-order kinetic model	916:948	The kinetic data and equilibrium adsorption behaviors were well depicted by pesudo-second-order kinetic model and Langmuir isotherm model, respectively.					
31790736	4	85	theme	adsorption	703:712	arg1	capacity					714:721	the adsorption capacity	699:721	the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2	699:763	Compared with CTS@Fe3O4, CTS@SnO2@Fe3O4 showed better adsorption performance for RBR, represented by the adsorption capacity reaching a maximum of 981.23 mg/g at pH 2, illustrating that the introduction of SnO2 was beneficial for adsorption.					
31790736	2	86	theme	obtained	305:312	arg1	material					314:321	the obtained material	301:321	the obtained material	301:321	The physicochemical properties of the obtained material were characterized by FTIR, XRD, VSM, TGA, SEM and N2 adsorption-desorption.					
31790736	3	87	from	Effects	400:406	arg1	adsorption					503:512	the adsorption	499:512	the adsorption of reactive brilliant red (RBR)	499:544	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
31790736	3	88	theme	concentration	466:478	arg1	Effects					400:406	Effects	400:406	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR)	400:544	Effects of contact time, solution pH, ionic strength, initial dye concentration and temperature on the adsorption of reactive brilliant red (RBR) were investigated via batch adsorption experiments.					
30848905	3	0	theme	membrane	634:641	arg1	representative					729:742	representative	729:742	representative	729:742	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	0	theme	membrane	634:641	arg1	OmpA					643:646	the E. coli outer membrane OmpA	616:646	the E. coli outer membrane OmpA	616:646	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	1	from	OmpF	667:670	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	1	from	OmpF	667:670	arg1	proteins					602:609	six proteins	598:609	six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	598:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	1	from	OmpF	667:670	arg1	range					687:691	a range	685:691	a range of membrane environments	685:716	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	2	theme	E.	620:621	arg1	representative					729:742	representative	729:742	representative	729:742	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	2	theme	E.	620:621	arg1	OmpA					643:646	the E. coli outer membrane OmpA	616:646	the E. coli outer membrane OmpA	616:646	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	3	theme	in	751:752	arg1	conditions					759:768	the in vivo conditions	747:768	the in vivo conditions for different strains of E. coli	747:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	1	4	theme	phospholipids	225:237	arg1	mixture					214:220	a mixture	212:220	a mixture of phospholipids in the inner leaflet	212:258	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	1	4	theme	phospholipids	225:237	arg1	molecules					308:316	almost exclusively lipopolysaccharide (LPS) molecules	264:316	almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet	264:337	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	3	5	theme	coarse-grained	549:562	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	6	theme	membrane	696:703	arg1	environments					705:716	membrane environments	696:716	membrane environments	696:716	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	1	7	theme	outer	325:329	arg1	leaflet					331:337	the outer leaflet	321:337	the outer leaflet	321:337	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	3	8	from	EstA	677:680	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	8	from	EstA	677:680	arg1	proteins					602:609	six proteins	598:609	six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	598:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	8	from	EstA	677:680	arg1	range					687:691	a range	685:691	a range of membrane environments	685:716	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	4	9	theme	LPS	965:967	arg1	pattern					843:849	a unique pattern	834:849	a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein	834:949	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	9	theme	LPS	965:967	arg1	level					956:960	the level	952:960	the level of LPS in the outer leaflet	952:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	9	theme	LPS	965:967	arg1	mobilities					1009:1018	the differing mobilities	995:1018	the differing mobilities of the lipids	995:1032	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	10	theme	interaction	854:864	arg1	pattern					843:849	a unique pattern	834:849	a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein	834:949	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	10	theme	interaction	854:864	arg1	level					956:960	the level	952:960	the level of LPS in the outer leaflet	952:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	10	theme	interaction	854:864	arg1	mobilities					1009:1018	the differing mobilities	995:1018	the differing mobilities of the lipids	995:1032	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	3	11	theme	environments	705:716	arg1	range					687:691	a range	685:691	a range of membrane environments	685:716	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	12	from	simulations	583:593	arg1	OmpX					649:652	OmpX	649:652	OmpX	649:652	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	12	from	simulations	583:593	arg1	representative					729:742	representative	729:742	representative	729:742	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	12	from	simulations	583:593	arg1	OmpF					667:670	OmpF	667:670	OmpF	667:670	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	12	from	simulations	583:593	arg1	EstA					677:680	EstA	677:680	EstA	677:680	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	12	from	simulations	583:593	arg1	BtuB					655:658	BtuB	655:658	BtuB	655:658	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	12	from	simulations	583:593	arg1	FhuA					661:664	FhuA	661:664	FhuA	661:664	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	12	from	simulations	583:593	arg1	OmpA					643:646	the E. coli outer membrane OmpA	616:646	the E. coli outer membrane OmpA	616:646	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	13	from	BtuB	655:658	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	13	from	BtuB	655:658	arg1	proteins					602:609	six proteins	598:609	six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	598:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	13	from	BtuB	655:658	arg1	range					687:691	a range	685:691	a range of membrane environments	685:716	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	4	14	theme	membrane	1061:1068	arg1	leaflets					1045:1052	the two leaflets	1037:1052	the two leaflets of the membrane	1037:1068	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	2	15	contain	having	471:476	arg1	smallest					462:469	the smallest	458:469	the smallest having eight strands to larger barrels composed of 22 strands	458:531	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	2	15	contain	having	471:476	arg2	strands					484:490	eight strands	478:490	eight strands	478:490	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	1	16	theme	Gram-negative	130:142	arg1	bacteria					144:151	Gram-negative bacteria	130:151	Gram-negative bacteria	130:151	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	4	17	from	leaflet	982:988	arg1	pattern					843:849	a unique pattern	834:849	a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein	834:949	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	17	from	leaflet	982:988	arg1	level					956:960	the level	952:960	the level of LPS in the outer leaflet	952:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	17	from	leaflet	982:988	arg1	mobilities					1009:1018	the differing mobilities	995:1018	the differing mobilities of the lipids	995:1032	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	3	18	dep	in	751:752	arg1	vivo					754:757	vivo	754:757	vivo	754:757	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	2	19	theme	proteins	396:403	arg1	range					387:391	a wide range	380:391	a wide range	380:391	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	3	20	theme	different	774:782	arg1	strains					784:790	different strains	774:790	different strains of E. coli	774:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	1	21	theme	bacteria	144:151	arg1	membrane					118:125	The outer membrane	108:125	The outer membrane of Gram-negative bacteria	108:151	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	0	22	theme	Membrane	6:13	arg1	OmpX					58:61	OmpX	58:61	OmpX	58:61	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	22	theme	Membrane	6:13	arg1	Proteins					15:22	Outer Membrane Proteins	0:22	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX	0:61	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	22	theme	Membrane	6:13	arg1	OmpA					24:27	OmpA	24:27	OmpA	24:27	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	22	theme	Membrane	6:13	arg1	EstA					42:45	EstA	42:45	EstA	42:45	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	22	theme	Membrane	6:13	arg1	BtuB					48:51	BtuB	48:51	BtuB	48:51	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	22	theme	Membrane	6:13	arg1	FhuA					30:33	FhuA	30:33	FhuA	30:33	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	22	theme	Membrane	6:13	arg1	OmpF					36:39	OmpF	36:39	OmpF	36:39	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	3	23	from	FhuA	661:664	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	23	from	FhuA	661:664	arg1	proteins					602:609	six proteins	598:609	six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	598:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	23	from	FhuA	661:664	arg1	range					687:691	a range	685:691	a range of membrane environments	685:716	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	24	dep	E.	620:621	arg1	coli					623:626	coli	623:626	coli	623:626	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	4	25	theme	outer	976:980	arg1	leaflet					982:988	the outer leaflet	972:988	the outer leaflet	972:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	0	26	theme	Outer	0:4	arg1	OmpX					58:61	OmpX	58:61	OmpX	58:61	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	26	theme	Outer	0:4	arg1	Proteins					15:22	Outer Membrane Proteins	0:22	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX	0:61	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	26	theme	Outer	0:4	arg1	OmpA					24:27	OmpA	24:27	OmpA	24:27	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	26	theme	Outer	0:4	arg1	EstA					42:45	EstA	42:45	EstA	42:45	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	26	theme	Outer	0:4	arg1	BtuB					48:51	BtuB	48:51	BtuB	48:51	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	26	theme	Outer	0:4	arg1	FhuA					30:33	FhuA	30:33	FhuA	30:33	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	26	theme	Outer	0:4	arg1	OmpF					36:39	OmpF	36:39	OmpF	36:39	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	1	27	theme	inner	246:250	arg1	leaflet					252:258	the inner leaflet	242:258	the inner leaflet	242:258	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	1	28	from	leaflet	252:258	arg1	mixture					214:220	a mixture	212:220	a mixture of phospholipids in the inner leaflet	212:258	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	1	28	from	leaflet	252:258	arg1	molecules					308:316	almost exclusively lipopolysaccharide (LPS) molecules	264:316	almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet	264:337	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	5	29	from	μs	1113:1114	arg1	analyses					1090:1097	analyses	1090:1097	analyses from over 200 μs of simulation for each protein	1090:1145	Overall we present analyses from over 200 μs of simulation for each protein.					
30848905	3	30	from	OmpX	649:652	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	30	from	OmpX	649:652	arg1	proteins					602:609	six proteins	598:609	six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	598:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	30	from	OmpX	649:652	arg1	range					687:691	a range	685:691	a range of membrane environments	685:716	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	0	31	theme	Lipopolysaccharide	75:92	arg1	Fingerprints					94:105	Unique Lipopolysaccharide Fingerprints	68:105	Unique Lipopolysaccharide Fingerprints	68:105	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	4	32	with	interaction	854:864	arg1	membrane					887:894	the surrounding membrane	871:894	the surrounding membrane	871:894	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	5	33	theme	simulation	1119:1128	arg1	μs					1113:1114	over 200 μs	1104:1114	over 200 μs of simulation for each protein	1104:1145	Overall we present analyses from over 200 μs of simulation for each protein.					
30848905	4	34	from	LPS	965:967	arg1	leaflet					982:988	the outer leaflet	972:988	the outer leaflet	972:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	2	35	theme	wide	382:385	arg1	range					387:391	a wide range	380:391	a wide range	380:391	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	0	36	theme	Unique	68:73	arg1	Fingerprints					94:105	Unique Lipopolysaccharide Fingerprints	68:105	Unique Lipopolysaccharide Fingerprints	68:105	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	3	37	theme	coli	798:801	arg1	strains					784:790	different strains	774:790	different strains of E. coli	774:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	1	38	theme	complex	166:172	arg1	architecture					187:198	a highly complex asymmetrical architecture	157:198	a highly complex asymmetrical architecture	157:198	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	2	39	theme	barrel	414:419	arg1	architecture					421:432	a β barrel architecture	410:432	a β barrel architecture	410:432	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	1	40	contain	has	153:155	arg2	architecture					187:198	a highly complex asymmetrical architecture	157:198	a highly complex asymmetrical architecture	157:198	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	1	40	contain	has	153:155	arg1	membrane					118:125	The outer membrane	108:125	The outer membrane of Gram-negative bacteria	108:151	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	3	41	from	OmpA	643:646	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	41	from	OmpA	643:646	arg1	proteins					602:609	six proteins	598:609	six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	598:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	41	from	OmpA	643:646	arg1	range					687:691	a range	685:691	a range of membrane environments	685:716	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	1	42	theme	asymmetrical	174:185	arg1	architecture					187:198	a highly complex asymmetrical architecture	157:198	a highly complex asymmetrical architecture	157:198	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	2	43	theme	β	412:412	arg1	architecture					421:432	a β barrel architecture	410:432	a β barrel architecture	410:432	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	1	44	from	phospholipids	225:237	arg1	leaflet					252:258	the inner leaflet	242:258	the inner leaflet	242:258	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	3	45	theme	molecular	564:572	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	4	46	from	level	956:960	arg1	leaflet					982:988	the outer leaflet	972:988	the outer leaflet	972:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	47	contain	has	830:832	arg1	protein					822:828	each protein	817:828	each protein	817:828	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	47	contain	has	830:832	arg2	pattern					843:849	a unique pattern	834:849	a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein	834:949	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	47	contain	has	830:832	arg2	mobilities					1009:1018	the differing mobilities	995:1018	the differing mobilities of the lipids	995:1032	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	47	contain	has	830:832	arg2	level					956:960	the level	952:960	the level of LPS in the outer leaflet	952:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	2	48	theme	outer	356:360	arg1	membrane					362:369	the outer membrane	352:369	the outer membrane	352:369	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	4	49	theme	surrounding	875:885	arg1	membrane					887:894	the surrounding membrane	871:894	the surrounding membrane	871:894	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	1	50	theme	lipopolysaccharide	283:300	arg1	molecules					308:316	almost exclusively lipopolysaccharide (LPS) molecules	264:316	almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet	264:337	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	3	51	theme	dynamics	574:581	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	1	52	contain	containing	201:210	arg2	mixture					214:220	a mixture	212:220	a mixture of phospholipids in the inner leaflet	212:258	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	1	52	contain	containing	201:210	arg2	molecules					308:316	almost exclusively lipopolysaccharide (LPS) molecules	264:316	almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet	264:337	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	1	52	contain	containing	201:210	arg1	architecture					187:198	a highly complex asymmetrical architecture	157:198	a highly complex asymmetrical architecture	157:198	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	0	53	contain	Have	63:66	arg1	OmpX					58:61	OmpX	58:61	OmpX	58:61	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	53	contain	Have	63:66	arg1	Proteins					15:22	Outer Membrane Proteins	0:22	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX	0:61	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	53	contain	Have	63:66	arg1	OmpA					24:27	OmpA	24:27	OmpA	24:27	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	53	contain	Have	63:66	arg1	EstA					42:45	EstA	42:45	EstA	42:45	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	53	contain	Have	63:66	arg2	Fingerprints					94:105	Unique Lipopolysaccharide Fingerprints	68:105	Unique Lipopolysaccharide Fingerprints	68:105	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	53	contain	Have	63:66	arg1	BtuB					48:51	BtuB	48:51	BtuB	48:51	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	53	contain	Have	63:66	arg1	FhuA					30:33	FhuA	30:33	FhuA	30:33	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	53	contain	Have	63:66	arg1	OmpF					36:39	OmpF	36:39	OmpF	36:39	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	1	54	from	molecules	308:316	arg1	leaflet					331:337	the outer leaflet	321:337	the outer leaflet	321:337	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	1	54	from	molecules	308:316	arg1	leaflet					252:258	the inner leaflet	242:258	the inner leaflet	242:258	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	1	55	from	mixture	214:220	arg1	leaflet					331:337	the outer leaflet	321:337	the outer leaflet	321:337	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	1	55	from	mixture	214:220	arg1	leaflet					252:258	the inner leaflet	242:258	the inner leaflet	242:258	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	4	56	from	mobilities	1009:1018	arg1	leaflet					982:988	the outer leaflet	972:988	the outer leaflet	972:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	2	57	with	proteins	396:403	arg1	architecture					421:432	a β barrel architecture	410:432	a β barrel architecture	410:432	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	2	58	theme	larger	495:500	arg1	barrels					502:508	larger barrels	495:508	larger barrels composed of 22 strands	495:531	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	4	59	theme	lipids	1027:1032	arg1	pattern					843:849	a unique pattern	834:849	a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein	834:949	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	59	theme	lipids	1027:1032	arg1	level					956:960	the level	952:960	the level of LPS in the outer leaflet	952:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	59	theme	lipids	1027:1032	arg1	mobilities					1009:1018	the differing mobilities	995:1018	the differing mobilities of the lipids	995:1032	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	2	60	contain	contains	371:378	arg1	membrane					362:369	the outer membrane	352:369	the outer membrane	352:369	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	2	60	contain	contains	371:378	arg2	membrane					362:369	the outer membrane	352:369	the outer membrane	352:369	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	2	60	contain	contains	371:378	arg1	coli					346:349	E. coli	343:349	E. coli	343:349	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	2	60	contain	contains	371:378	arg2	range					387:391	a wide range	380:391	a wide range	380:391	In E. coli, the outer membrane contains a wide range of proteins with a β barrel architecture, that vary in size from the smallest having eight strands to larger barrels composed of 22 strands.					
30848905	0	61	dep	Proteins	15:22	arg1	OmpX					58:61	OmpX	58:61	OmpX	58:61	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	61	dep	Proteins	15:22	arg1	Proteins					15:22	Outer Membrane Proteins	0:22	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX	0:61	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	61	dep	Proteins	15:22	arg1	OmpA					24:27	OmpA	24:27	OmpA	24:27	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	61	dep	Proteins	15:22	arg1	EstA					42:45	EstA	42:45	EstA	42:45	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	61	dep	Proteins	15:22	arg1	BtuB					48:51	BtuB	48:51	BtuB	48:51	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	61	dep	Proteins	15:22	arg1	FhuA					30:33	FhuA	30:33	FhuA	30:33	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	0	61	dep	Proteins	15:22	arg1	OmpF					36:39	OmpF	36:39	OmpF	36:39	Outer Membrane Proteins OmpA, FhuA, OmpF, EstA, BtuB, and OmpX Have Unique Lipopolysaccharide Fingerprints.					
30848905	4	62	from	pattern	843:849	arg1	leaflet					982:988	the outer leaflet	972:988	the outer leaflet	972:988	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	4	63	theme	unique	836:841	arg1	pattern					843:849	a unique pattern	834:849	a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein	834:949	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	3	64	theme	proteins	602:609	arg1	simulations					583:593	coarse-grained molecular dynamics simulations	549:593	coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli	549:801	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	4	65	theme	protein	943:949	arg1	composition					924:934	the composition	920:934	the composition of the protein	920:949	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	3	66	theme	outer	628:632	arg1	representative					729:742	representative	729:742	representative	729:742	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	66	theme	outer	628:632	arg1	OmpA					643:646	the E. coli outer membrane OmpA	616:646	the E. coli outer membrane OmpA	616:646	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	4	67	theme	differing	999:1007	arg1	mobilities					1009:1018	the differing mobilities	995:1018	the differing mobilities of the lipids	995:1032	We show that each protein has a unique pattern of interaction with the surrounding membrane, which is influenced by the composition of the protein, the level of LPS in the outer leaflet, and the differing mobilities of the lipids in the two leaflets of the membrane.					
30848905	1	68	theme	outer	112:116	arg1	membrane					118:125	The outer membrane	108:125	The outer membrane of Gram-negative bacteria	108:151	The outer membrane of Gram-negative bacteria has a highly complex asymmetrical architecture, containing a mixture of phospholipids in the inner leaflet and almost exclusively lipopolysaccharide (LPS) molecules in the outer leaflet.					
30848905	3	69	theme	conditions	759:768	arg1	representative					729:742	representative	729:742	representative	729:742	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
30848905	3	69	theme	conditions	759:768	arg1	OmpA					643:646	the E. coli outer membrane OmpA	616:646	the E. coli outer membrane OmpA	616:646	Here we report coarse-grained molecular dynamics simulations of six proteins from the E. coli outer membrane OmpA, OmpX, BtuB, FhuA, OmpF, and EstA in a range of membrane environments, which are representative of the in vivo conditions for different strains of E. coli.					
29728770	5	0	theme	histological	726:737	arg1	composition					746:756	the histological tissue composition	722:756	the histological tissue composition	722:756	To validate the tissue quality, the histological tissue composition was visualized by glycosaminoglycan and collagen staining.					
29728770	7	1	theme	better	994:999	arg1	strategies					1018:1027	better cartilage repair strategies	994:1027	better cartilage repair strategies	994:1027	From this knowledge, better cartilage repair strategies could be tailored in the future.					
29728770	5	2	theme	tissue	739:744	arg1	composition					746:756	the histological tissue composition	722:756	the histological tissue composition	722:756	To validate the tissue quality, the histological tissue composition was visualized by glycosaminoglycan and collagen staining.					
29728770	6	3	theme	structures	926:935	arg1	properties					905:914	the zone-dependent mechanical properties	875:914	the zone-dependent mechanical properties of graded structures, such as human articular cartilage	875:970	This work aims to introduce a new protocol to investigate the zone-dependent mechanical properties of graded structures, such as human articular cartilage.					
29728770	3	4	theme	human	448:452	arg1	cartilage					470:478	human femoral condyle cartilage	448:478	human femoral condyle cartilage	448:478	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	3	5	theme	local	423:427	arg1	modulus					437:443	local elastic modulus	423:443	local elastic modulus of human femoral condyle cartilage	423:478	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	4	6	theme	superficial	547:557	arg1	zone					559:562	the cartilage superficial zone	533:562	the cartilage superficial zone towards the calcified layer	533:590	From the cartilage superficial zone towards the calcified layer, a gradient of elastic modulus values between 0.020 ± 0.003 MPa and 6.44 ± 1.02 MPa was measured.					
29728770	3	7	theme	zone-dependent	398:411	arg1	change					413:418	a zone-dependent change	396:418	a zone-dependent change of local elastic modulus of human femoral condyle cartilage	396:478	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	4	8	theme	cartilage	537:545	arg1	zone					559:562	the cartilage superficial zone	533:562	the cartilage superficial zone towards the calcified layer	533:590	From the cartilage superficial zone towards the calcified layer, a gradient of elastic modulus values between 0.020 ± 0.003 MPa and 6.44 ± 1.02 MPa was measured.					
29728770	5	9	theme	tissue	706:711	arg1	quality					713:719	the tissue quality	702:719	the tissue quality	702:719	To validate the tissue quality, the histological tissue composition was visualized by glycosaminoglycan and collagen staining.					
29728770	3	10	theme	instrumented	492:503	arg1	technique					517:525	an instrumented indentation technique	489:525	an instrumented indentation technique	489:525	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	0	11	theme	cartilage	56:64	arg1	properties					26:35	Zone-dependent mechanical properties	0:35	Zone-dependent mechanical properties of human articular cartilage	0:64	Zone-dependent mechanical properties of human articular cartilage obtained by indentation measurements.					
29728770	3	12	theme	cartilage	470:478	arg1	modulus					437:443	local elastic modulus	423:443	local elastic modulus of human femoral condyle cartilage	423:478	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	6	13	theme	mechanical	894:903	arg1	properties					905:914	the zone-dependent mechanical properties	875:914	the zone-dependent mechanical properties of graded structures, such as human articular cartilage	875:970	This work aims to introduce a new protocol to investigate the zone-dependent mechanical properties of graded structures, such as human articular cartilage.					
29728770	2	14	theme	articular	335:343	arg1	cartilage					345:353	human articular cartilage	329:353	human articular cartilage	329:353	However, information about gradients of mechanical properties in human articular cartilage is limited.					
29728770	3	15	theme	indentation	505:515	arg1	technique					517:525	an instrumented indentation technique	489:525	an instrumented indentation technique	489:525	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	0	16	theme	mechanical	15:24	arg1	properties					26:35	Zone-dependent mechanical properties	0:35	Zone-dependent mechanical properties of human articular cartilage	0:64	Zone-dependent mechanical properties of human articular cartilage obtained by indentation measurements.					
29728770	6	17	theme	zone-dependent	879:892	arg1	properties					905:914	the zone-dependent mechanical properties	875:914	the zone-dependent mechanical properties of graded structures, such as human articular cartilage	875:970	This work aims to introduce a new protocol to investigate the zone-dependent mechanical properties of graded structures, such as human articular cartilage.					
29728770	1	18	theme	Emerging	104:111	arg1	technology					125:134	Emerging 3D printing technology	104:134	Emerging 3D printing technology	104:134	Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation.					
29728770	2	19	from	gradients	291:299	arg1	cartilage					345:353	human articular cartilage	329:353	human articular cartilage	329:353	However, information about gradients of mechanical properties in human articular cartilage is limited.					
29728770	1	20	theme	mechanical	232:241	arg1	adaptation					252:261	layer-by-layer mechanical property adaptation	217:261	layer-by-layer mechanical property adaptation	217:261	Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation.					
29728770	0	21	theme	Zone-dependent	0:13	arg1	properties					26:35	Zone-dependent mechanical properties	0:35	Zone-dependent mechanical properties of human articular cartilage	0:64	Zone-dependent mechanical properties of human articular cartilage obtained by indentation measurements.					
29728770	4	22	theme	values	623:628	arg1	gradient					595:602	a gradient	593:602	a gradient of elastic modulus values between 0.020 ± 0.003 MPa and 6.44 ± 1.02 MPa	593:674	From the cartilage superficial zone towards the calcified layer, a gradient of elastic modulus values between 0.020 ± 0.003 MPa and 6.44 ± 1.02 MPa was measured.					
29728770	1	23	theme	3D	113:114	arg1	technology					125:134	Emerging 3D printing technology	104:134	Emerging 3D printing technology	104:134	Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation.					
29728770	1	24	theme	property	243:250	arg1	adaptation					252:261	layer-by-layer mechanical property adaptation	217:261	layer-by-layer mechanical property adaptation	217:261	Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation.					
29728770	3	25	theme	elastic	429:435	arg1	modulus					437:443	local elastic modulus	423:443	local elastic modulus of human femoral condyle cartilage	423:478	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	4	26	theme	modulus	615:621	arg1	values					623:628	elastic modulus values	607:628	elastic modulus values	607:628	From the cartilage superficial zone towards the calcified layer, a gradient of elastic modulus values between 0.020 ± 0.003 MPa and 6.44 ± 1.02 MPa was measured.					
29728770	5	27	theme	collagen	798:805	arg1	staining					807:814	glycosaminoglycan and collagen staining	776:814	glycosaminoglycan and collagen staining	776:814	To validate the tissue quality, the histological tissue composition was visualized by glycosaminoglycan and collagen staining.					
29728770	1	28	theme	printing	116:123	arg1	technology					125:134	Emerging 3D printing technology	104:134	Emerging 3D printing technology	104:134	Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation.					
29728770	3	29	theme	modulus	437:443	arg1	change					413:418	a zone-dependent change	396:418	a zone-dependent change of local elastic modulus of human femoral condyle cartilage	396:478	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	0	30	theme	articular	46:54	arg1	cartilage					56:64	human articular cartilage	40:64	human articular cartilage	40:64	Zone-dependent mechanical properties of human articular cartilage obtained by indentation measurements.					
29728770	7	31	theme	repair	1011:1016	arg1	strategies					1018:1027	better cartilage repair strategies	994:1027	better cartilage repair strategies	994:1027	From this knowledge, better cartilage repair strategies could be tailored in the future.					
29728770	0	32	theme	human	40:44	arg1	cartilage					56:64	human articular cartilage	40:64	human articular cartilage	40:64	Zone-dependent mechanical properties of human articular cartilage obtained by indentation measurements.					
29728770	3	33	theme	femoral	454:460	arg1	cartilage					470:478	human femoral condyle cartilage	448:478	human femoral condyle cartilage	448:478	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	6	34	theme	articular	952:960	arg1	cartilage					962:970	human articular cartilage	946:970	human articular cartilage	946:970	This work aims to introduce a new protocol to investigate the zone-dependent mechanical properties of graded structures, such as human articular cartilage.					
29728770	3	35	theme	condyle	462:468	arg1	cartilage					470:478	human femoral condyle cartilage	448:478	human femoral condyle cartilage	448:478	In this study, we quantified a zone-dependent change of local elastic modulus of human femoral condyle cartilage by using an instrumented indentation technique.					
29728770	4	36	theme	elastic	607:613	arg1	modulus					615:621	elastic modulus	607:621	elastic modulus values	607:628	From the cartilage superficial zone towards the calcified layer, a gradient of elastic modulus values between 0.020 ± 0.003 MPa and 6.44 ± 1.02 MPa was measured.					
29728770	6	37	theme	human	946:950	arg1	cartilage					962:970	human articular cartilage	946:970	human articular cartilage	946:970	This work aims to introduce a new protocol to investigate the zone-dependent mechanical properties of graded structures, such as human articular cartilage.					
29728770	6	38	theme	new	847:849	arg1	protocol					851:858	a new protocol	845:858	a new protocol to investigate the zone-dependent mechanical properties of graded structures, such as human articular cartilage	845:970	This work aims to introduce a new protocol to investigate the zone-dependent mechanical properties of graded structures, such as human articular cartilage.					
29728770	1	39	theme	innovative	144:153	arg1	approaches					155:164	innovative approaches	144:164	innovative approaches	144:164	Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation.					
29728770	5	40	theme	glycosaminoglycan	776:792	arg1	staining					807:814	glycosaminoglycan and collagen staining	776:814	glycosaminoglycan and collagen staining	776:814	To validate the tissue quality, the histological tissue composition was visualized by glycosaminoglycan and collagen staining.					
29728770	2	41	theme	human	329:333	arg1	cartilage					345:353	human articular cartilage	329:353	human articular cartilage	329:353	However, information about gradients of mechanical properties in human articular cartilage is limited.					
29728770	6	42	theme	graded	919:924	arg1	cartilage					962:970	human articular cartilage	946:970	human articular cartilage	946:970	This work aims to introduce a new protocol to investigate the zone-dependent mechanical properties of graded structures, such as human articular cartilage.					
29728770	6	42	theme	graded	919:924	arg1	structures					926:935	graded structures	919:935	graded structures	919:935	This work aims to introduce a new protocol to investigate the zone-dependent mechanical properties of graded structures, such as human articular cartilage.					
29728770	7	43	theme	cartilage	1001:1009	arg1	strategies					1018:1027	better cartilage repair strategies	994:1027	better cartilage repair strategies	994:1027	From this knowledge, better cartilage repair strategies could be tailored in the future.					
29728770	0	44	theme	indentation	78:88	arg1	measurements					90:101	indentation measurements	78:101	indentation measurements	78:101	Zone-dependent mechanical properties of human articular cartilage obtained by indentation measurements.					
29728770	2	45	theme	properties	315:324	arg1	gradients					291:299	gradients	291:299	gradients of mechanical properties in human articular cartilage	291:353	However, information about gradients of mechanical properties in human articular cartilage is limited.					
29728770	1	46	theme	layer-by-layer	217:230	arg1	adaptation					252:261	layer-by-layer mechanical property adaptation	217:261	layer-by-layer mechanical property adaptation	217:261	Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation.					
29728770	4	47	theme	calcified	576:584	arg1	layer					586:590	the calcified layer	572:590	the calcified layer	572:590	From the cartilage superficial zone towards the calcified layer, a gradient of elastic modulus values between 0.020 ± 0.003 MPa and 6.44 ± 1.02 MPa was measured.					
29728770	2	48	theme	mechanical	304:313	arg1	properties					315:324	mechanical properties	304:324	mechanical properties	304:324	However, information about gradients of mechanical properties in human articular cartilage is limited.					
29728770	1	49	theme	cartilage	181:189	arg1	scaffolds					191:199	cartilage scaffolds	181:199	cartilage scaffolds associated with layer-by-layer mechanical property adaptation	181:261	Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation.					
31431549	0	0	theme	Antibiotic	80:89	arg1	Testing					106:112	Antibiotic Susceptibility Testing	80:112	Antibiotic Susceptibility Testing	80:112	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing.					
31431549	5	1	theme	diversity	812:820	arg1	susceptibility					792:805	the antibiotic susceptibility	777:805	the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods	777:903	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	5	2	from	less	845:848	arg1	susceptibility					792:805	the antibiotic susceptibility	777:805	the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods	777:903	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	2	3	theme	bacterial	316:324	arg1	cells					326:330	bacterial cells	316:330	bacterial cells	316:330	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	4	4	theme	Escherichia	633:643	arg1	cells					650:654	Escherichia coli cells	633:654	Escherichia coli cells	633:654	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	0	5	theme	Susceptibility	91:104	arg1	Testing					106:112	Antibiotic Susceptibility Testing	80:112	Antibiotic Susceptibility Testing	80:112	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing.					
31431549	4	6	theme	deleterious	668:678	arg1	effects					680:686	any deleterious effects	664:686	any deleterious effects on cell physiology	664:705	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	8	7	theme	simple	1412:1417	arg1	implementation					1419:1432	their simple implementation	1406:1432	their simple implementation	1406:1432	Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use.					
31431549	6	8	theme	microfluidic	1052:1063	arg1	techniques					1065:1074	Current microfluidic techniques	1044:1074	Current microfluidic techniques	1044:1074	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	5	9	theme	antibiotic	781:790	arg1	susceptibility					792:805	the antibiotic susceptibility	777:805	the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods	777:903	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	8	10	theme	low	1374:1376	arg1	cost					1378:1381	the low cost	1370:1381	the low cost of chitosan slides	1370:1400	Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use.					
31431549	3	11	theme	chitosan	498:505	arg1	polymers					507:514	chitosan polymers	498:514	chitosan polymers	498:514	In this work, we chemically characterized a class of chitosan polymers for their biocompatibility when adsorbed to glass.					
31431549	7	12	theme	Chitosan	1223:1230	arg1	films					1232:1236	Chitosan films	1223:1236	Chitosan films	1223:1236	Chitosan films are fully biocompatible and could thus be a viable replacement for existing commercial devices that currently use polylysine.					
31431549	2	13	theme	bacterial	423:431	arg1	physiology					433:442	bacterial physiology	423:442	bacterial physiology	423:442	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	6	14	theme	chitosan	915:922	arg1	polymers					924:931	chitosan polymers	915:931	chitosan polymers	915:931	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	6	15	theme	Current	1044:1050	arg1	techniques					1065:1074	Current microfluidic techniques	1044:1074	Current microfluidic techniques	1044:1074	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	6	16	theme	clinical	1011:1018	arg1	significance.IMPORTANCE					1020:1042	clinical significance.IMPORTANCE	1011:1042	clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation	1011:1220	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	4	17	theme	length	592:597	arg1	chains					576:581	Chitosan chains	567:581	Chitosan chains of known length and composition	567:613	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	6	18	theme	antibiotic	1139:1148	arg1	treatment					1150:1158	antibiotic treatment	1139:1158	antibiotic treatment	1139:1158	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	0	19	theme	Microfluidic	19:30	arg1	Studies					32:38	Microfluidic Studies	19:38	Microfluidic Studies of Single Bacteria	19:57	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing.					
31431549	4	20	theme	known	586:590	arg1	length					592:597	known length	586:597	known length	586:597	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	5	21	theme	higher	868:873	arg1	accuracy					875:882	higher accuracy	868:882	higher accuracy than current methods	868:903	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	4	22	theme	composition	603:613	arg1	chains					576:581	Chitosan chains	567:581	Chitosan chains of known length and composition	567:613	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	4	23	dep	Escherichia	633:643	arg1	coli					645:648	coli	645:648	coli	645:648	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	1	24	theme	Single-cell	115:125	arg1	microfluidics					127:139	Single-cell microfluidics	115:139	Single-cell microfluidics	115:139	Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment.					
31431549	1	24	theme	Single-cell	115:125	arg1	method					155:160	a powerful method	144:160	a powerful method to study bacteria and determine their susceptibility to antibiotic treatment	144:237	Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment.					
31431549	4	25	from	effects	680:686	arg1	physiology					696:705	cell physiology	691:705	cell physiology	691:705	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	1	26	theme	antibiotic	218:227	arg1	treatment					229:237	antibiotic treatment	218:237	antibiotic treatment	218:237	Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment.					
31431549	5	27	theme	clinical	825:832	arg1	strains					834:840	clinical strains	825:840	clinical strains	825:840	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	6	28	theme	complex	1201:1207	arg1	manipulation					1209:1220	their complex manipulation	1195:1220	their complex manipulation	1195:1220	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	5	29	from	susceptibility	792:805	arg1	less					845:848	less	845:848	less	845:848	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	2	30	theme	adhesive	259:266	arg1	molecules					268:276	adhesive molecules	259:276	adhesive molecules	259:276	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	4	31	theme	cell	691:694	arg1	physiology					696:705	cell physiology	691:705	cell physiology	691:705	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	5	32	theme	strains	834:840	arg1	diversity					812:820	a diversity	810:820	a diversity of clinical strains in less than 1 h and with higher accuracy than current methods	810:903	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	4	33	theme	Chitosan	567:574	arg1	chains					576:581	Chitosan chains	567:581	Chitosan chains of known length and composition	567:613	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	5	34	theme	current	889:895	arg1	methods					897:903	current methods	889:903	current methods	889:903	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	0	35	theme	Bacteria	50:57	arg1	Perspectives					63:74	Perspectives	63:74	Perspectives for Antibiotic Susceptibility Testing	63:112	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing.					
31431549	0	35	theme	Bacteria	50:57	arg1	Studies					32:38	Microfluidic Studies	19:38	Microfluidic Studies of Single Bacteria	19:57	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing.					
31431549	6	36	theme	bacterial	989:997	arg1	pathogen					999:1006	another bacterial pathogen	981:1006	another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation	981:1220	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	6	36	theme	bacterial	989:997	arg1	pneumoniae					969:978	Klebsiella pneumoniae	958:978	Klebsiella pneumoniae	958:978	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	6	37	theme	significance.IMPORTANCE	1020:1042	arg1	pathogen					999:1006	another bacterial pathogen	981:1006	another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation	981:1220	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	6	37	theme	significance.IMPORTANCE	1020:1042	arg1	pneumoniae					969:978	Klebsiella pneumoniae	958:978	Klebsiella pneumoniae	958:978	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	8	38	theme	clinical	1485:1492	arg1	use					1494:1496	clinical use	1485:1496	research as well as clinical use	1465:1496	Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use.					
31431549	2	39	theme	cationic	372:379	arg1	polymers					381:388	traditional cationic polymers	360:388	traditional cationic polymers such as polylysine	360:407	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	2	39	theme	cationic	372:379	arg1	polylysine					398:407	polylysine	398:407	polylysine	398:407	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	0	40	theme	Single	43:48	arg1	Bacteria					50:57	Single Bacteria	43:57	Single Bacteria	43:57	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing.					
31431549	5	41	theme	machine	724:730	arg1	learning					732:739	a machine learning	722:739	a machine learning approach	722:748	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	5	42	from	diversity	812:820	arg1	less					845:848	less	845:848	less	845:848	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	2	43	theme	Glass	240:244	arg1	solution					293:300	a potential solution	281:300	a potential solution to immobilize bacterial cells and perform microscopy	281:353	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	2	43	theme	Glass	240:244	arg1	treatment					246:254	Glass treatment	240:254	Glass treatment by adhesive molecules	240:276	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	2	44	theme	traditional	360:370	arg1	polymers					381:388	traditional cationic polymers	360:388	traditional cationic polymers such as polylysine	360:407	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	2	44	theme	traditional	360:370	arg1	polylysine					398:407	polylysine	398:407	polylysine	398:407	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	5	45	theme	learning	732:739	arg1	approach					741:748	a machine learning approach	722:748	a machine learning approach	722:748	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	5	46	with	diversity	812:820	arg1	accuracy					875:882	higher accuracy	868:882	higher accuracy than current methods	868:903	Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods.					
31431549	2	47	theme	potential	283:291	arg1	solution					293:300	a potential solution	281:300	a potential solution to immobilize bacterial cells and perform microscopy	281:353	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	2	47	theme	potential	283:291	arg1	treatment					246:254	Glass treatment	240:254	Glass treatment by adhesive molecules	240:276	Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology.					
31431549	3	48	theme	polymers	507:514	arg1	class					489:493	a class	487:493	a class of chitosan polymers for their biocompatibility	487:541	In this work, we chemically characterized a class of chitosan polymers for their biocompatibility when adsorbed to glass.					
31431549	4	49	theme	cells	650:654	arg1	growth					623:628	growth	623:628	growth of Escherichia coli cells	623:654	Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology.					
31431549	1	50	theme	powerful	146:153	arg1	microfluidics					127:139	Single-cell microfluidics	115:139	Single-cell microfluidics	115:139	Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment.					
31431549	1	50	theme	powerful	146:153	arg1	method					155:160	a powerful method	144:160	a powerful method to study bacteria and determine their susceptibility to antibiotic treatment	144:237	Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment.					
31431549	6	51	dep	significance.IMPORTANCE	1020:1042	arg1	powerful					1080:1087	powerful	1080:1087	powerful	1080:1087	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	6	51	dep	significance.IMPORTANCE	1020:1042	arg1	limited					1184:1190	limited	1184:1190	are currently limited by their complex manipulation	1170:1220	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	8	52	theme	slides	1395:1400	arg1	cost					1378:1381	the low cost	1370:1381	the low cost of chitosan slides	1370:1400	Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use.					
31431549	8	52	theme	slides	1395:1400	arg1	implementation					1419:1432	their simple implementation	1406:1432	their simple implementation	1406:1432	Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use.					
31431549	7	53	theme	commercial	1314:1323	arg1	devices					1325:1331	commercial devices	1314:1331	commercial devices that currently use polylysine	1314:1361	Chitosan films are fully biocompatible and could thus be a viable replacement for existing commercial devices that currently use polylysine.					
31431549	6	54	theme	pneumoniae	969:978	arg1	growth					948:953	growth	948:953	growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation	948:1220	Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance.IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation.					
31431549	8	55	theme	chitosan	1386:1393	arg1	slides					1395:1400	chitosan slides	1386:1400	chitosan slides	1386:1400	Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use.					
31431549	7	56	theme	viable	1282:1287	arg1	replacement					1289:1299	a viable replacement	1280:1299	a viable replacement for existing commercial devices that currently use polylysine	1280:1361	Chitosan films are fully biocompatible and could thus be a viable replacement for existing commercial devices that currently use polylysine.					
31843417	15	0	theme	GAG	2261:2263	arg1	content					2265:2271	a lower GAG content	2253:2271	a lower GAG content in patients with TMJ disorders	2253:2302	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	1	1	from	content	356:362	arg1	tissues					378:384	connective tissues	367:384	connective tissues	367:384	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	3	2	theme	MRI	560:562	arg1	dGEMRIC					575:581	dGEMRIC	575:581	dGEMRIC	575:581	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	3	2	theme	MRI	560:562	arg1	technique					564:572	the compositional MRI technique	542:572	the compositional MRI technique	542:572	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	14	3	theme	suitable	2110:2117	arg1	method					2102:2107	a useful, compositional, quantitative method	2064:2107	a useful, compositional, quantitative method	2064:2107	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	14	3	theme	suitable	2110:2117	arg1	dGEMRIC					2044:2050	CONCLUSIONS dGEMRIC	2032:2050	CONCLUSIONS dGEMRIC	2032:2050	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	0	4	theme	preliminary	208:218	arg1	results					220:226	preliminary results	208:226	preliminary results	208:226	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	13	5	theme	pre-contrast	1916:1927	arg1	value					1929:1933	initial pre-contrast value	1908:1933	initial pre-contrast value	1908:1933	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	3	6	theme	dGEMRIC	777:783	arg1	index					785:789	the dGEMRIC index	773:789	the dGEMRIC index of patients to the healthy volunteers	773:827	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	3	7	theme	early	608:612	arg1	diagnosis					598:606	diagnosis early	598:612	diagnosis early	598:612	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	6	8	theme	control	1127:1133	arg1	group					1135:1139	a control group	1125:1139	a control group	1125:1139	Volunteers were used as a control group.					
31843417	6	8	theme	control	1127:1133	arg1	Volunteers					1101:1110	Volunteers	1101:1110	Volunteers	1101:1110	Volunteers were used as a control group.					
31843417	13	9	from	drop	1971:1974	arg1	group					1954:1958	control group	1946:1958	control group	1946:1958	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	0	10	theme	temporomandibular	129:145	arg1	disorders					147:155	temporomandibular disorders	129:155	temporomandibular disorders	129:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	9	11	theme	T1	1440:1441	arg1	maps					1443:1446	T1 maps	1440:1446	T1 maps	1440:1446	T1 maps were calculated offline and ROIs were drawn on TMJ discs by a specialist trained in TMD disorders.					
31843417	15	12	from	results	2185:2191	arg1	disc					2231:2234	the articular disc	2217:2234	the articular disc of the TMJ	2217:2245	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	1	13	dep	cartilage	275:283	arg1	method					313:318	a quantitative method	298:318	a quantitative method for assessment of glycosaminoglycan content in connective tissues	298:384	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	15	14	from	content	2265:2271	arg1	patients					2276:2283	patients	2276:2283	patients with TMJ disorders	2276:2302	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	8	15	theme	dGEMRIC	1422:1428	arg1	protocol					1430:1437	the dGEMRIC protocol	1418:1437	the dGEMRIC protocol	1418:1437	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	8	16	theme	recovery	1271:1278	arg1	sequence					1285:1292	The Inversion recovery (IR) sequence	1257:1292	The Inversion recovery (IR) sequence	1257:1292	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	0	17	theme	gadolinium-enhanced	171:189	arg1	MRI					191:193	delayed gadolinium-enhanced MRI	163:193	delayed gadolinium-enhanced MRI	163:193	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	13	18	theme	initial	2003:2009	arg1	value					2024:2028	initial pre-contrast value	2003:2028	initial pre-contrast value	2003:2028	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	3	19	theme	degenerative	618:629	arg1	changes					631:637	the degenerative changes	614:637	the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD)	614:756	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	15	20	from	index	2208:2212	arg1	disc					2231:2234	the articular disc	2217:2234	the articular disc of the TMJ	2217:2245	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	0	21	from	patients	115:122	arg1	disc					76:79	the articular/fibrocartilaginous disc	43:79	the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders	43:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	11	22	theme	patients	1711:1718	arg1	discs					1698:1702	the TMJ articular discs	1680:1702	the TMJ articular discs of the patients and the volunteers	1680:1737	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	0	23	from	joint	106:110	arg1	patients					115:122	patients	115:122	patients with temporomandibular disorders	115:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	3	24	theme	healthy	810:816	arg1	volunteers					818:827	the healthy volunteers	806:827	the healthy volunteers	806:827	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	11	25	dep	significant	1757:1767	arg1	P = .019					1770:1777	P = .019	1770:1777	P = .019	1770:1777	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	11	25	dep	significant	1757:1767	arg1	significant					1757:1767	significant	1757:1767	significant	1757:1767	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	11	25	dep	significant	1757:1767	arg1	difference					1639:1648	The difference	1635:1648	The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers	1635:1737	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	4	26	dep	men	858:860	arg1	20.8÷28.1 years					875:889	20.8÷28.1 years	875:889	20.8÷28.1 years	875:889	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	6	27	used	used	1117:1120	arg2	Volunteers					1101:1110	Volunteers	1101:1110	Volunteers	1101:1110	Volunteers were used as a control group.					
31843417	6	27	used	used	1117:1120	arg2	group					1135:1139	a control group	1125:1139	a control group	1125:1139	Volunteers were used as a control group.					
31843417	0	28	dep	using	157:161	arg1	results					220:226	preliminary results	208:226	preliminary results	208:226	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	15	29	from	disc	2231:2234	arg1	results					2185:2191	The results	2181:2191	The results of the dGEMRIC index in the articular disc of the TMJ	2181:2245	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	2	30	from	joint	476:480	arg1	diagnosis					417:425	the early diagnosis	407:425	the early diagnosis of degenerative changes in the temporomandibular joint	407:480	We hypothesize that the early diagnosis of degenerative changes in the temporomandibular joint could be diagnosed using dGEMRIC technique.					
31843417	7	31	theme	DESS	1187:1190	arg1	sequence					1193:1200	the 3D GRE (DESS) sequence	1175:1200	the 3D GRE (DESS) sequence	1175:1200	The PD-weighted FSE sequence and the 3D GRE (DESS) sequence protocols were performed for morphological assessment.					
31843417	9	32	theme	TMJ	1495:1497	arg1	discs					1499:1503	TMJ discs	1495:1503	TMJ discs	1495:1503	T1 maps were calculated offline and ROIs were drawn on TMJ discs by a specialist trained in TMD disorders.					
31843417	13	33	dep	stronger	1888:1895	arg1	%					1901:1901	-54%	1898:1901	-54% from initial pre-contrast value	1898:1933	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	14	34	theme	CONCLUSIONS	2032:2042	arg1	method					2102:2107	a useful, compositional, quantitative method	2064:2107	a useful, compositional, quantitative method	2064:2107	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	14	34	theme	CONCLUSIONS	2032:2042	arg1	dGEMRIC					2044:2050	CONCLUSIONS dGEMRIC	2032:2050	CONCLUSIONS dGEMRIC	2032:2050	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	8	35	theme	T1	1312:1313	arg1	measurements					1331:1342	T1 relaxation time measurements	1312:1342	T1 relaxation time measurements	1312:1342	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	0	36	theme	joint	106:110	arg1	disc					76:79	the articular/fibrocartilaginous disc	43:79	the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders	43:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	13	37	theme	T1	1968:1969	arg1	drop					1971:1974	the T1 drop	1964:1974	the T1 drop less pronounced	1964:1990	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	11	38	from	values	1670:1675	arg1	discs					1698:1702	the TMJ articular discs	1680:1702	the TMJ articular discs of the patients and the volunteers	1680:1737	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	8	39	theme	time	1326:1329	arg1	measurements					1331:1342	T1 relaxation time measurements	1312:1342	T1 relaxation time measurements	1312:1342	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	2	40	theme	degenerative	430:441	arg1	changes					443:449	degenerative changes	430:449	degenerative changes in the temporomandibular joint	430:480	We hypothesize that the early diagnosis of degenerative changes in the temporomandibular joint could be diagnosed using dGEMRIC technique.					
31843417	15	41	theme	lower	2255:2259	arg1	content					2265:2271	a lower GAG content	2253:2271	a lower GAG content in patients with TMJ disorders	2253:2302	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	0	42	from	changes	32:38	arg1	disc					76:79	the articular/fibrocartilaginous disc	43:79	the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders	43:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	0	43	theme	Early	0:4	arg1	diagnosis					6:14	Early diagnosis	0:14	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders	0:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	14	44	dep	useful	2066:2071	arg1	compositional					2074:2086	compositional	2074:2086	compositional	2074:2086	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	14	44	dep	useful	2066:2071	arg1	quantitative					2089:2100	quantitative	2089:2100	quantitative	2089:2100	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	2	45	theme	temporomandibular	458:474	arg1	joint					476:480	the temporomandibular joint	454:480	the temporomandibular joint	454:480	We hypothesize that the early diagnosis of degenerative changes in the temporomandibular joint could be diagnosed using dGEMRIC technique.					
31843417	7	46	theme	PD-weighted	1146:1156	arg1	sequence					1162:1169	The PD-weighted FSE sequence	1142:1169	The PD-weighted FSE sequence	1142:1169	The PD-weighted FSE sequence and the 3D GRE (DESS) sequence protocols were performed for morphological assessment.					
31843417	0	47	theme	changes	32:38	arg1	diagnosis					6:14	Early diagnosis	0:14	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders	0:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	11	48	dep	RESULTS	1627:1633	arg1	P = .019					1770:1777	P = .019	1770:1777	P = .019	1770:1777	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	11	48	dep	RESULTS	1627:1633	arg1	significant					1757:1767	significant	1757:1767	significant	1757:1767	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	11	48	dep	RESULTS	1627:1633	arg1	difference					1639:1648	The difference	1635:1648	The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers	1635:1737	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	5	49	theme	Only	1001:1004	arg1	patients					1006:1013	Only patients	1001:1013	Only patients with no morphological abnormality on MRI and without disc dislocations	1001:1084	Only patients with no morphological abnormality on MRI and without disc dislocations were included.					
31843417	2	50	from	diagnosis	417:425	arg1	joint					476:480	the temporomandibular joint	454:480	the temporomandibular joint	454:480	We hypothesize that the early diagnosis of degenerative changes in the temporomandibular joint could be diagnosed using dGEMRIC technique.					
31843417	2	51	theme	dGEMRIC	507:513	arg1	technique					515:523	dGEMRIC technique	507:523	dGEMRIC technique	507:523	We hypothesize that the early diagnosis of degenerative changes in the temporomandibular joint could be diagnosed using dGEMRIC technique.					
31843417	3	52	theme	temporomandibular	677:693	arg1	joint					695:699	the temporomandibular joint	673:699	the temporomandibular joint (TMJ)	673:705	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	3	52	theme	temporomandibular	677:693	arg1	TMJ					702:704	TMJ	702:704	TMJ	702:704	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	8	53	theme	agent	1375:1379	arg1	administration					1381:1394	intra-venous (IV) contrast agent administration	1348:1394	intra-venous (IV) contrast agent administration	1348:1394	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	1	54	theme	glycosaminoglycan	338:354	arg1	content					356:362	glycosaminoglycan content	338:362	glycosaminoglycan content in connective tissues	338:384	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	15	55	theme	TMJ	2290:2292	arg1	disorders					2294:2302	TMJ disorders	2290:2302	TMJ disorders	2290:2302	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	12	56	theme	agent	1796:1800	arg1	administration					1802:1815	contrast agent administration	1787:1815	contrast agent administration	1787:1815	After contrast agent administration the T1 values dropped in both groups.					
31843417	3	57	theme	fibrocartilaginous	646:663	arg1	disc					665:668	the fibrocartilaginous disc	642:668	the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD)	642:756	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	13	58	theme	pre-contrast	2011:2022	arg1	value					2024:2028	initial pre-contrast value	2003:2028	initial pre-contrast value	2003:2028	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	15	59	theme	index	2208:2212	arg1	results					2185:2191	The results	2181:2191	The results of the dGEMRIC index in the articular disc of the TMJ	2181:2245	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	12	60	theme	T1	1821:1822	arg1	values					1824:1829	the T1 values	1817:1829	the T1 values	1817:1829	After contrast agent administration the T1 values dropped in both groups.					
31843417	13	61	from	value	1929:1933	arg1	%					1901:1901	-54%	1898:1901	-54% from initial pre-contrast value	1898:1933	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	13	62	from	value	2024:2028	arg1	%					1996:1996	-46%	1993:1996	-46% from initial pre-contrast value	1993:2028	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	11	63	theme	TMJ	1684:1686	arg1	discs					1698:1702	the TMJ articular discs	1680:1702	the TMJ articular discs of the patients and the volunteers	1680:1737	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	4	64	theme	METHODS	830:836	arg1	volunteers					842:851	METHODS Six volunteers	830:851	METHODS Six volunteers (two men, four women; 20.8÷28.1 years)	830:890	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	8	65	used	used	1400:1403	arg2	administration					1381:1394	intra-venous (IV) contrast agent administration	1348:1394	intra-venous (IV) contrast agent administration	1348:1394	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	14	66	theme	small	2128:2132	arg1	joints					2134:2139	small joints	2128:2139	small joints	2128:2139	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	14	66	theme	small	2128:2132	arg1	disc					2164:2167	the articular disc	2150:2167	the articular disc of the TMJ	2150:2178	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	4	67	dep	patients	903:910	arg1	men					940:942	four men	935:942	four men	935:942	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	4	67	dep	patients	903:910	arg1	women					928:932	22 TMJs, seven women	913:932	women	928:932	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	3	68	theme	compositional	546:558	arg1	dGEMRIC					575:581	dGEMRIC	575:581	dGEMRIC	575:581	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	3	68	theme	compositional	546:558	arg1	technique					564:572	the compositional MRI technique	542:572	the compositional MRI technique	542:572	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	11	69	theme	dGEMRIC	1662:1668	arg1	values					1670:1675	the dGEMRIC values	1658:1675	the dGEMRIC values in the TMJ articular discs of the patients and the volunteers	1658:1737	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	5	70	with	patients	1006:1013	arg1	abnormality					1037:1047	no morphological abnormality	1020:1047	no morphological abnormality on MRI	1020:1054	Only patients with no morphological abnormality on MRI and without disc dislocations were included.					
31843417	3	71	theme	temporomandibular	724:740	arg1	disorders					742:750	temporomandibular disorders	724:750	temporomandibular disorders (TMD)	724:756	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	3	71	theme	temporomandibular	724:740	arg1	TMD					753:755	TMD	753:755	TMD	753:755	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	1	72	from	assessment	324:333	arg1	tissues					378:384	connective tissues	367:384	connective tissues	367:384	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	13	73	theme	initial	1908:1914	arg1	value					1929:1933	initial pre-contrast value	1908:1933	initial pre-contrast value	1908:1933	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	0	74	from	disc	76:79	arg1	patients					115:122	patients	115:122	patients with temporomandibular disorders	115:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	0	74	from	disc	76:79	arg1	diagnosis					6:14	Early diagnosis	0:14	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders	0:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	0	75	with	patients	115:122	arg1	disorders					147:155	temporomandibular disorders	129:155	temporomandibular disorders	129:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	3	76	with	patients	710:717	arg1	disorders					742:750	temporomandibular disorders	724:750	temporomandibular disorders (TMD)	724:756	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	3	76	with	patients	710:717	arg1	TMD					753:755	TMD	753:755	TMD	753:755	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	10	77	theme	Statistical	1547:1557	arg1	evaluation					1559:1568	Statistical evaluation	1547:1568	Statistical evaluation	1547:1568	Statistical evaluation was performed by ANOVA and correlations were calculated.					
31843417	4	78	theme	TMJs	916:919	arg1	men					940:942	four men	935:942	four men	935:942	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	4	78	theme	TMJs	916:919	arg1	women					928:932	22 TMJs, seven women	913:932	women	928:932	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	14	79	theme	useful	2066:2071	arg1	method					2102:2107	a useful, compositional, quantitative method	2064:2107	a useful, compositional, quantitative method	2064:2107	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	14	79	theme	useful	2066:2071	arg1	dGEMRIC					2044:2050	CONCLUSIONS dGEMRIC	2032:2050	CONCLUSIONS dGEMRIC	2032:2050	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	0	80	theme	delayed	163:169	arg1	MRI					191:193	delayed gadolinium-enhanced MRI	163:193	delayed gadolinium-enhanced MRI	163:193	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	1	81	theme	Delayed	240:246	arg1	gadolinium					248:257	BACKGROUND Delayed gadolinium	229:257	BACKGROUND Delayed gadolinium	229:257	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	13	82	dep	drop	1971:1974	arg1	%					1996:1996	-46%	1993:1996	-46% from initial pre-contrast value	1993:2028	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	4	83	dep	women	928:932	arg1	24÷54 years					945:955	24÷54 years	945:955	24÷54 years	945:955	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	5	84	theme	disc	1068:1071	arg1	dislocations					1073:1084	disc dislocations	1068:1084	disc dislocations	1068:1084	Only patients with no morphological abnormality on MRI and without disc dislocations were included.					
31843417	8	85	theme	Inversion	1261:1269	arg1	IR					1281:1282	IR	1281:1282	IR	1281:1282	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	8	85	theme	Inversion	1261:1269	arg1	recovery					1271:1278	Inversion recovery	1261:1278	The Inversion recovery (IR) sequence	1257:1292	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	3	86	from	Tesla	589:593	arg1	dGEMRIC					575:581	dGEMRIC	575:581	dGEMRIC	575:581	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	3	86	from	Tesla	589:593	arg1	technique					564:572	the compositional MRI technique	542:572	the compositional MRI technique	542:572	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	3	87	theme	patients	794:801	arg1	index					785:789	the dGEMRIC index	773:789	the dGEMRIC index of patients to the healthy volunteers	773:827	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	5	88	theme	morphological	1023:1035	arg1	abnormality					1037:1047	no morphological abnormality	1020:1047	no morphological abnormality on MRI	1020:1054	Only patients with no morphological abnormality on MRI and without disc dislocations were included.					
31843417	13	89	theme	T1	1880:1881	arg1	drop					1883:1886	the T1 drop	1876:1886	the T1 drop	1876:1886	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	5	90	from	abnormality	1037:1047	arg1	MRI					1052:1054	MRI	1052:1054	MRI	1052:1054	Only patients with no morphological abnormality on MRI and without disc dislocations were included.					
31843417	15	91	theme	articular	2221:2229	arg1	disc					2231:2234	the articular disc	2217:2234	the articular disc of the TMJ	2217:2245	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	15	92	with	patients	2276:2283	arg1	disorders					2294:2302	TMJ disorders	2290:2302	TMJ disorders	2290:2302	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	7	93	theme	GRE	1182:1184	arg1	sequence					1193:1200	the 3D GRE (DESS) sequence	1175:1200	the 3D GRE (DESS) sequence	1175:1200	The PD-weighted FSE sequence and the 3D GRE (DESS) sequence protocols were performed for morphological assessment.					
31843417	0	94	theme	temporomandibular	88:104	arg1	joint					106:110	the temporomandibular joint	84:110	the temporomandibular joint in patients with temporomandibular disorders	84:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	1	95	theme	connective	367:376	arg1	tissues					378:384	connective tissues	367:384	connective tissues	367:384	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	3	96	dep	diagnosis	598:606	arg1	changes					631:637	the degenerative changes	614:637	the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD)	614:756	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	0	97	from	diagnosis	6:14	arg1	disc					76:79	the articular/fibrocartilaginous disc	43:79	the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders	43:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	13	98	theme	patient	1858:1864	arg1	group					1866:1870	patient group	1858:1870	patient group	1858:1870	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	7	99	theme	3D	1179:1180	arg1	sequence					1193:1200	the 3D GRE (DESS) sequence	1175:1200	the 3D GRE (DESS) sequence	1175:1200	The PD-weighted FSE sequence and the 3D GRE (DESS) sequence protocols were performed for morphological assessment.					
31843417	7	100	dep	sequence	1162:1169	arg1	protocols					1202:1210	protocols	1202:1210	protocols	1202:1210	The PD-weighted FSE sequence and the 3D GRE (DESS) sequence protocols were performed for morphological assessment.					
31843417	14	101	theme	TMJ	2176:2178	arg1	disc					2164:2167	the articular disc	2150:2167	the articular disc of the TMJ	2150:2178	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	11	102	theme	volunteers	1728:1737	arg1	discs					1698:1702	the TMJ articular discs	1680:1702	the TMJ articular discs of the patients and the volunteers	1680:1737	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	1	103	theme	BACKGROUND	229:238	arg1	gadolinium					248:257	BACKGROUND Delayed gadolinium	229:257	BACKGROUND Delayed gadolinium	229:257	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	15	104	theme	TMJ	2243:2245	arg1	disc					2231:2234	the articular disc	2217:2234	the articular disc of the TMJ	2217:2245	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	8	105	theme	relaxation	1315:1324	arg1	measurements					1331:1342	T1 relaxation time measurements	1312:1342	T1 relaxation time measurements	1312:1342	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	3	106	from	disc	665:668	arg1	patients					710:717	patients	710:717	patients with temporomandibular disorders (TMD)	710:756	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	7	107	theme	FSE	1158:1160	arg1	sequence					1162:1169	The PD-weighted FSE sequence	1142:1169	The PD-weighted FSE sequence	1142:1169	The PD-weighted FSE sequence and the 3D GRE (DESS) sequence protocols were performed for morphological assessment.					
31843417	13	108	theme	pronounced	1981:1990	arg1	drop					1971:1974	the T1 drop	1964:1974	the T1 drop less pronounced	1964:1990	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	14	109	theme	articular	2154:2162	arg1	disc					2164:2167	the articular disc	2150:2167	the articular disc of the TMJ	2150:2178	CONCLUSIONS dGEMRIC seems to be a useful, compositional, quantitative method, suitable also for small joints, such as the articular disc of the TMJ.					
31843417	2	110	theme	early	411:415	arg1	diagnosis					417:425	the early diagnosis	407:425	the early diagnosis of degenerative changes in the temporomandibular joint	407:480	We hypothesize that the early diagnosis of degenerative changes in the temporomandibular joint could be diagnosed using dGEMRIC technique.					
31843417	0	111	theme	degenerative	19:30	arg1	changes					32:38	degenerative changes	19:38	degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders	19:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	1	112	theme	quantitative	300:311	arg1	method					313:318	a quantitative method	298:318	a quantitative method for assessment of glycosaminoglycan content in connective tissues	298:384	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	9	113	theme	TMD	1532:1534	arg1	disorders					1536:1544	TMD disorders	1532:1544	TMD disorders	1532:1544	T1 maps were calculated offline and ROIs were drawn on TMJ discs by a specialist trained in TMD disorders.					
31843417	3	114	theme	joint	695:699	arg1	disc					665:668	the fibrocartilaginous disc	642:668	the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD)	642:756	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	8	115	theme	intra-venous	1348:1359	arg1	administration					1381:1394	intra-venous (IV) contrast agent administration	1348:1394	intra-venous (IV) contrast agent administration	1348:1394	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	3	116	from	changes	631:637	arg1	disc					665:668	the fibrocartilaginous disc	642:668	the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD)	642:756	PURPOSE To test the compositional MRI technique, dGEMRIC, at 3 Tesla to diagnosis early the degenerative changes in the fibrocartilaginous disc of the temporomandibular joint (TMJ) in patients with temporomandibular disorders (TMD) and to compare the dGEMRIC index of patients to the healthy volunteers.					
31843417	1	117	from	tissues	378:384	arg1	assessment					324:333	assessment	324:333	assessment of glycosaminoglycan content in connective tissues	324:384	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	4	118	dep	volunteers	842:851	arg1	men					858:860	two men	854:860	two men	854:860	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	4	118	dep	volunteers	842:851	arg1	women					868:872	four women	863:872	four women	863:872	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	13	119	theme	control	1946:1952	arg1	group					1954:1958	control group	1946:1958	control group	1946:1958	In patient group was the T1 drop stronger (-54% from initial pre-contrast value), while in control group was the T1 drop less pronounced (-46% from initial pre-contrast value).					
31843417	2	120	theme	changes	443:449	arg1	diagnosis					417:425	the early diagnosis	407:425	the early diagnosis of degenerative changes in the temporomandibular joint	407:480	We hypothesize that the early diagnosis of degenerative changes in the temporomandibular joint could be diagnosed using dGEMRIC technique.					
31843417	0	121	theme	articular/fibrocartilaginous	47:74	arg1	disc					76:79	the articular/fibrocartilaginous disc	43:79	the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders	43:155	Early diagnosis of degenerative changes in the articular/fibrocartilaginous disc of the temporomandibular joint in patients with temporomandibular disorders using delayed gadolinium-enhanced MRI at 3 Tesla - preliminary results.					
31843417	4	122	theme	prospective	982:992	arg1	trial					994:998	this prospective trial	977:998	this prospective trial	977:998	METHODS Six volunteers (two men, four women; 20.8÷28.1 years) and eleven patients (22 TMJs, seven women, four men; 24÷54 years) were recruited for this prospective trial.					
31843417	11	123	theme	articular	1688:1696	arg1	discs					1698:1702	the TMJ articular discs	1680:1702	the TMJ articular discs of the patients and the volunteers	1680:1737	RESULTS The difference between the dGEMRIC values in the TMJ articular discs of the patients and the volunteers was statistically significant (P = .019).					
31843417	8	124	theme	contrast	1366:1373	arg1	administration					1381:1394	intra-venous (IV) contrast agent administration	1348:1394	intra-venous (IV) contrast agent administration	1348:1394	The Inversion recovery (IR) sequence was performed for T1 relaxation time measurements and intra-venous (IV) contrast agent administration was used according to the dGEMRIC protocol.					
31843417	1	125	theme	content	356:362	arg1	assessment					324:333	assessment	324:333	assessment of glycosaminoglycan content in connective tissues	324:384	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31843417	12	126	theme	contrast	1787:1794	arg1	administration					1802:1815	contrast agent administration	1787:1815	contrast agent administration	1787:1815	After contrast agent administration the T1 values dropped in both groups.					
31843417	7	127	theme	morphological	1231:1243	arg1	assessment					1245:1254	morphological assessment	1231:1254	morphological assessment	1231:1254	The PD-weighted FSE sequence and the 3D GRE (DESS) sequence protocols were performed for morphological assessment.					
31843417	2	128	from	changes	443:449	arg1	joint					476:480	the temporomandibular joint	454:480	the temporomandibular joint	454:480	We hypothesize that the early diagnosis of degenerative changes in the temporomandibular joint could be diagnosed using dGEMRIC technique.					
31843417	15	129	theme	dGEMRIC	2200:2206	arg1	index					2208:2212	the dGEMRIC index	2196:2212	the dGEMRIC index in the articular disc of the TMJ	2196:2245	The results of the dGEMRIC index in the articular disc of the TMJ imply a lower GAG content in patients with TMJ disorders.					
31843417	1	130	theme	cartilage	275:283	arg1	MRI					268:270	MRI	268:270	MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues	268:384	BACKGROUND Delayed gadolinium enhanced MRI of cartilage (dGEMRIC) is a quantitative method for assessment of glycosaminoglycan content in connective tissues.					
31400696	6	0	theme	cryo-scanning	1461:1473	arg1	microscopy					1484:1493	cryo-scanning electron microscopy	1461:1493	cryo-scanning electron microscopy	1461:1493	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	1	1	theme	cellulose	390:398	arg1	network					361:367	the previously formed physical network	330:367	the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution	330:488	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	3	2	theme	mechanical	817:826	arg1	behavior					828:835	their mechanical behavior	811:835	their mechanical behavior	811:835	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	1	3	theme	hydrogels	167:175	arg1	types					124:128	Two types	120:128	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks	120:237	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	1	4	contain	possessing	177:186	arg1	hydrogels					167:175	stiff and high-strength composite hydrogels	133:175	stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks	133:237	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	1	4	contain	possessing	177:186	arg2	structure					192:200	the structure	188:200	the structure of interpenetrating polymer networks	188:237	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	1	5	theme	acrylamide	291:300	arg1	polymerization					273:286	free-radical polymerization	260:286	free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution	260:488	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	2	6	theme	hydrogels	530:538	arg1	behavior					506:513	The mechanical behavior	491:513	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values	491:622	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values was studied.					
31400696	3	7	theme	different	742:750	arg1	conditions					757:766	different test conditions	742:766	different test conditions	742:766	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	1	8	theme	reactive	472:479	arg1	solution					481:488	the reactive solution	468:488	the reactive solution	468:488	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	4	9	theme	BC-based	855:862	arg1	hydrogels					864:872	BC-based hydrogels	855:872	BC-based hydrogels	855:872	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	5	10	theme	BC	1330:1331	arg1	micro-fibrils					1333:1345	BC micro-fibrils	1330:1345	BC micro-fibrils	1330:1345	This effect can be explained by the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils.					
31400696	4	11	located	observed	1138:1145	arg1	region					1166:1171	the deformation region	1150:1171	the deformation region beyond 60%	1150:1182	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	11	located	observed	1138:1145	arg2	PC-					847:849	PC-	847:849	PC-	847:849	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	11	located	observed	1138:1145	arg2	hydrogels					864:872	BC-based hydrogels	855:872	BC-based hydrogels	855:872	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	0	12	theme	cellulose	109:117	arg1	type					101:104	the type	97:104	the type of cellulose	97:117	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	1	13	theme	bacterial	403:411	arg1	cellulose					413:421	bacterial cellulose	403:421	bacterial cellulose (PC and BC respectively)	403:446	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	6	14	theme	Submicron-	1348:1357	arg1	features					1385:1392	Submicron- and micron-scale specific features	1348:1392	Submicron- and micron-scale specific features of structures of composite hydrogels of both types	1348:1443	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	4	15	theme	hydrogels	1124:1132	arg1	stiffness					1098:1106	the stiffness	1094:1106	the stiffness of the BC-based hydrogels	1094:1132	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	16	theme	subsequent	1041:1050	arg1	compression					1052:1062	the subsequent compression	1037:1062	the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels	1037:1132	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	2	17	theme	compressive	567:577	arg1	deformations					579:590	compressive deformations	567:590	compressive deformations with different amplitude values	567:622	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values was studied.					
31400696	1	18	theme	cellulose	413:421	arg1	network					361:367	the previously formed physical network	330:367	the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution	330:488	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	4	19	dep	amplitude	931:939	arg1	%					949:949	%	949:949	%	949:949	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	6	20	theme	types	1439:1443	arg1	hydrogels					1421:1429	composite hydrogels	1411:1429	composite hydrogels of both types	1411:1443	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	6	21	theme	micron-scale	1363:1374	arg1	features					1385:1392	Submicron- and micron-scale specific features	1348:1392	Submicron- and micron-scale specific features of structures of composite hydrogels of both types	1348:1443	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	4	22	theme	dramatic	1073:1080	arg1	increase					1082:1089	the dramatic increase	1069:1089	the dramatic increase in the stiffness of the BC-based hydrogels	1069:1132	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	5	23	theme	intermolecular	1248:1261	arg1	structure					1263:1271	the intermolecular structure	1244:1271	the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils	1244:1345	This effect can be explained by the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils.					
31400696	4	24	with	compression	910:920	arg1	amplitude					931:939	the amplitude	927:939	the amplitude up to 80%	927:949	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	25	theme	tests	1002:1006	arg1	conditions					963:972	the conditions	959:972	the conditions of the multiple compression tests (cyclic compressions)	959:1028	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	0	26	theme	cellulose-polyacrylamide	14:37	arg1	hydrogels					39:47	High-strength cellulose-polyacrylamide hydrogels	0:47	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.	0:118	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	0	26	theme	cellulose-polyacrylamide	14:37	arg1	behavior					61:68	Mechanical behavior	50:68	Mechanical behavior	50:68	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	0	26	theme	cellulose-polyacrylamide	14:37	arg1	structure					74:82	structure	74:82	structure	74:82	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	3	27	theme	substantial	785:795	arg1	difference					797:806	the substantial difference	781:806	the substantial difference in their mechanical behavior	781:835	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	4	28	theme	cyclic	1009:1014	arg1	compressions					1016:1027	cyclic compressions	1009:1027	cyclic compressions	1009:1027	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	28	theme	cyclic	1009:1014	arg1	tests					1002:1006	the multiple compression tests	977:1006	the multiple compression tests (cyclic compressions)	977:1028	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	3	29	theme	test	752:755	arg1	conditions					757:766	different test conditions	742:766	different test conditions	742:766	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	0	30	theme	High-strength	0:12	arg1	hydrogels					39:47	High-strength cellulose-polyacrylamide hydrogels	0:47	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.	0:118	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	0	30	theme	High-strength	0:12	arg1	behavior					61:68	Mechanical behavior	50:68	Mechanical behavior	50:68	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	0	30	theme	High-strength	0:12	arg1	structure					74:82	structure	74:82	structure	74:82	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	5	31	theme	deep	1221:1224	arg1	reorganization					1226:1239	the deep reorganization	1217:1239	the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils	1217:1345	This effect can be explained by the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils.					
31400696	2	32	with	deformations	579:590	arg1	values					617:622	different amplitude values	597:622	different amplitude values	597:622	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values was studied.					
31400696	6	33	theme	hydrogels	1421:1429	arg1	structures					1397:1406	structures	1397:1406	structures of composite hydrogels of both types	1397:1443	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	1	34	theme	interpenetrating	205:220	arg1	networks					230:237	interpenetrating polymer networks	205:237	interpenetrating polymer networks	205:237	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	3	35	from	difference	797:806	arg1	behavior					828:835	their mechanical behavior	811:835	their mechanical behavior	811:835	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	4	36	theme	multiple	981:988	arg1	compressions					1016:1027	cyclic compressions	1009:1027	cyclic compressions	1009:1027	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	36	theme	multiple	981:988	arg1	tests					1002:1006	the multiple compression tests	977:1006	the multiple compression tests (cyclic compressions)	977:1028	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	37	theme	one-shot	901:908	arg1	compression					910:920	the one-shot compression	897:920	the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels	897:1132	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	1	38	theme	polymer	222:228	arg1	networks					230:237	interpenetrating polymer networks	205:237	interpenetrating polymer networks	205:237	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	6	39	theme	effects	1542:1548	arg1	peculiarities					1510:1522	the peculiarities	1506:1522	the peculiarities of the mechanical effects observed	1506:1557	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	2	40	theme	amplitude	607:615	arg1	values					617:622	different amplitude values	597:622	different amplitude values	597:622	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values was studied.					
31400696	3	41	theme	hydrogels	706:714	arg1	tests					693:697	compression tests	681:697	compression tests of the hydrogels of both types obtained in different test conditions	681:766	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	5	42	theme	micro-fibrils	1333:1345	arg1	reorientation					1313:1325	the stress-induced reorientation	1294:1325	the stress-induced reorientation of BC micro-fibrils	1294:1345	This effect can be explained by the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils.					
31400696	1	43	theme	networks	230:237	arg1	structure					192:200	the structure	188:200	the structure of interpenetrating polymer networks	188:237	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	2	44	theme	synthesized	518:528	arg1	hydrogels					530:538	synthesized hydrogels	518:538	synthesized hydrogels subjected to the action of compressive deformations with different amplitude values	518:622	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values was studied.					
31400696	1	45	theme	formed	345:350	arg1	network					361:367	the previously formed physical network	330:367	the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution	330:488	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	2	46	theme	different	597:605	arg1	values					617:622	different amplitude values	597:622	different amplitude values	597:622	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values was studied.					
31400696	4	47	theme	compression	990:1000	arg1	compressions					1016:1027	cyclic compressions	1009:1027	cyclic compressions	1009:1027	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	47	theme	compression	990:1000	arg1	tests					1002:1006	the multiple compression tests	977:1006	the multiple compression tests (cyclic compressions)	977:1028	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	5	48	theme	structure	1263:1271	arg1	reorganization					1226:1239	the deep reorganization	1217:1239	the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils	1217:1345	This effect can be explained by the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils.					
31400696	0	49	theme	Mechanical	50:59	arg1	hydrogels					39:47	High-strength cellulose-polyacrylamide hydrogels	0:47	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.	0:118	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	0	49	theme	Mechanical	50:59	arg1	behavior					61:68	Mechanical behavior	50:68	Mechanical behavior	50:68	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	1	50	theme	physical	352:359	arg1	network					361:367	the previously formed physical network	330:367	the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution	330:488	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	3	51	theme	tests	693:697	arg1	curves					671:676	the stress-strain curves	653:676	the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions	653:766	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	6	52	theme	composite	1411:1419	arg1	hydrogels					1421:1429	composite hydrogels	1411:1429	composite hydrogels of both types	1411:1443	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	3	53	theme	stress-strain	657:669	arg1	curves					671:676	the stress-strain curves	653:676	the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions	653:766	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	4	54	dep	%	949:949	arg1	80					947:948	80	947:948	80	947:948	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	2	55	theme	deformations	579:590	arg1	action					557:562	the action	553:562	the action of compressive deformations with different amplitude values	553:622	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values was studied.					
31400696	4	56	dep	compression	1052:1062	arg1	acts					1064:1067	acts	1064:1067	acts the dramatic increase in the stiffness of the BC-based hydrogels	1064:1132	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	57	from	compression	910:920	arg1	conditions					963:972	the conditions	959:972	the conditions of the multiple compression tests (cyclic compressions)	959:1028	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	6	58	theme	mechanical	1531:1540	arg1	effects					1542:1548	the mechanical effects	1527:1548	the mechanical effects observed	1527:1557	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	2	59	theme	mechanical	495:504	arg1	behavior					506:513	The mechanical behavior	491:513	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values	491:622	The mechanical behavior of synthesized hydrogels subjected to the action of compressive deformations with different amplitude values was studied.					
31400696	1	60	dep	cellulose	413:421	arg1	BC					431:432	BC	431:432	BC	431:432	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	1	60	dep	cellulose	413:421	arg1	PC					424:425	PC	424:425	PC	424:425	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	4	61	from	increase	1082:1089	arg1	stiffness					1098:1106	the stiffness	1094:1106	the stiffness of the BC-based hydrogels	1094:1132	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	1	62	theme	regenerated	372:382	arg1	cellulose					390:398	regenerated plant cellulose	372:398	regenerated plant cellulose	372:398	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	3	63	theme	compression	681:691	arg1	tests					693:697	compression tests	681:697	compression tests of the hydrogels of both types obtained in different test conditions	681:766	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	1	64	theme	stiff	133:137	arg1	hydrogels					167:175	stiff and high-strength composite hydrogels	133:175	stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks	133:237	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	5	65	theme	material	1280:1287	arg1	structure					1263:1271	the intermolecular structure	1244:1271	the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils	1244:1345	This effect can be explained by the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils.					
31400696	6	66	theme	specific	1376:1383	arg1	features					1385:1392	Submicron- and micron-scale specific features	1348:1392	Submicron- and micron-scale specific features of structures of composite hydrogels of both types	1348:1443	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	0	67	dep	hydrogels	39:47	arg1	hydrogels					39:47	High-strength cellulose-polyacrylamide hydrogels	0:47	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.	0:118	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	0	67	dep	hydrogels	39:47	arg1	behavior					61:68	Mechanical behavior	50:68	Mechanical behavior	50:68	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	0	67	dep	hydrogels	39:47	arg1	structure					74:82	structure	74:82	structure	74:82	High-strength cellulose-polyacrylamide hydrogels: Mechanical behavior and structure depending on the type of cellulose.					
31400696	4	68	dep	80	947:948	arg1	to					944:945	to	944:945	to	944:945	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	69	dep	PC-	847:849	arg1	withstand					874:882	withstand	874:882	withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels	874:1132	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	4	70	theme	deformation	1154:1164	arg1	region					1166:1171	the deformation region	1150:1171	the deformation region beyond 60%	1150:1182	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	1	71	theme	free-radical	260:271	arg1	polymerization					273:286	free-radical polymerization	260:286	free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution	260:488	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	3	72	theme	types	724:728	arg1	hydrogels					706:714	the hydrogels	702:714	the hydrogels of both types obtained in different test conditions	702:766	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	3	73	theme	curves	671:676	arg1	analysis					641:648	The analysis	637:648	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions	637:766	The analysis of the stress-strain curves of compression tests of the hydrogels of both types obtained in different test conditions demonstrates the substantial difference in their mechanical behavior.					
31400696	1	74	theme	high-strength	143:155	arg1	hydrogels					167:175	stiff and high-strength composite hydrogels	133:175	stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks	133:237	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	6	75	theme	structures	1397:1406	arg1	features					1385:1392	Submicron- and micron-scale specific features	1348:1392	Submicron- and micron-scale specific features of structures of composite hydrogels of both types	1348:1443	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	6	76	theme	electron	1475:1482	arg1	microscopy					1484:1493	cryo-scanning electron microscopy	1461:1493	cryo-scanning electron microscopy	1461:1493	Submicron- and micron-scale specific features of structures of composite hydrogels of both types were studied by cryo-scanning electron microscopy to explain the peculiarities of the mechanical effects observed.					
31400696	1	77	theme	plant	384:388	arg1	cellulose					390:398	regenerated plant cellulose	372:398	regenerated plant cellulose	372:398	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	5	78	with	material	1280:1287	arg1	reorientation					1313:1325	the stress-induced reorientation	1294:1325	the stress-induced reorientation of BC micro-fibrils	1294:1345	This effect can be explained by the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils.					
31400696	1	79	theme	composite	157:165	arg1	hydrogels					167:175	stiff and high-strength composite hydrogels	133:175	stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks	133:237	Two types of stiff and high-strength composite hydrogels possessing the structure of interpenetrating polymer networks were synthesized via free-radical polymerization of acrylamide carried out straight within the previously formed physical network of regenerated plant cellulose or bacterial cellulose (PC and BC respectively) that was swollen in the reactive solution.					
31400696	4	80	theme	BC-based	1115:1122	arg1	hydrogels					1124:1132	the BC-based hydrogels	1111:1132	the BC-based hydrogels	1111:1132	Both the PC- and BC-based hydrogels withstand successfully the one-shot compression with the amplitude up to 80%, but in the conditions of the multiple compression tests (cyclic compressions) during the subsequent compression acts the dramatic increase in the stiffness of the BC-based hydrogels was observed at the deformation region beyond 60%.					
31400696	5	81	theme	stress-induced	1298:1311	arg1	reorientation					1313:1325	the stress-induced reorientation	1294:1325	the stress-induced reorientation of BC micro-fibrils	1294:1345	This effect can be explained by the deep reorganization of the intermolecular structure of the material with the stress-induced reorientation of BC micro-fibrils.					
29447965	0	0	theme	acid	97:100	arg1	dyes					102:105	acid dyes	97:105	acid dyes	97:105	Simple fabrication of Chitosan/Graphene nanoplates composite spheres for efficient adsorption of acid dyes from aqueous solution.					
29447965	5	1	theme	resulting	693:701	arg1	230.91mgg-1					733:743	230.91mgg-1	733:743	230.91mgg-1	733:743	The resulting adsorption capacity for MO is 230.91mgg-1 and 132.94mgg-1 for AR1, respectively.					
29447965	5	1	theme	resulting	693:701	arg1	capacity					714:721	The resulting adsorption capacity	689:721	The resulting adsorption capacity for MO	689:728	The resulting adsorption capacity for MO is 230.91mgg-1 and 132.94mgg-1 for AR1, respectively.					
29447965	7	2	theme	dye	998:1000	arg1	removal					1002:1008	the dye removal	994:1008	the dye removal	994:1008	Therefore, CS/GNPs is the apromising adsorbent with non-toxic, efficient, low-cost and easy to prepare for the dye removal.					
29447965	0	3	theme	dyes	102:105	arg1	adsorption					83:92	efficient adsorption	73:92	efficient adsorption of acid dyes from aqueous solution	73:127	Simple fabrication of Chitosan/Graphene nanoplates composite spheres for efficient adsorption of acid dyes from aqueous solution.					
29447965	2	4	theme	electron	308:315	arg1	TEM					329:331	TEM	329:331	TEM	329:331	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	2	4	theme	electron	308:315	arg1	microscopy					317:326	transmission electron microscopy	295:326	transmission electron microscopy (TEM)	295:332	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	3	5	theme	methyl	479:484	arg1	orange					486:491	methyl orange	479:491	methyl orange (MO)	479:496	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	3	5	theme	methyl	479:484	arg1	MO					494:495	MO	494:495	MO	494:495	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	2	6	theme	transmission	295:306	arg1	TEM					329:331	TEM	329:331	TEM	329:331	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	2	6	theme	transmission	295:306	arg1	microscopy					317:326	transmission electron microscopy	295:326	transmission electron microscopy (TEM)	295:332	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	1	7	theme	crosslinked	169:179	arg1	CS/GNPs					228:234	CS/GNPs	228:234	CS/GNPs	228:234	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	1	7	theme	crosslinked	169:179	arg1	sphere					220:225	crosslinked chitosan/graphene nanoplates composite sphere	169:225	crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs)	169:235	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	0	8	theme	aqueous	112:118	arg1	solution					120:127	aqueous solution	112:127	aqueous solution	112:127	Simple fabrication of Chitosan/Graphene nanoplates composite spheres for efficient adsorption of acid dyes from aqueous solution.					
29447965	5	9	theme	adsorption	703:712	arg1	230.91mgg-1					733:743	230.91mgg-1	733:743	230.91mgg-1	733:743	The resulting adsorption capacity for MO is 230.91mgg-1 and 132.94mgg-1 for AR1, respectively.					
29447965	5	9	theme	adsorption	703:712	arg1	capacity					714:721	The resulting adsorption capacity	689:721	The resulting adsorption capacity for MO	689:728	The resulting adsorption capacity for MO is 230.91mgg-1 and 132.94mgg-1 for AR1, respectively.					
29447965	1	10	theme	chitosan/graphene	181:197	arg1	CS/GNPs					228:234	CS/GNPs	228:234	CS/GNPs	228:234	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	1	10	theme	chitosan/graphene	181:197	arg1	sphere					220:225	crosslinked chitosan/graphene nanoplates composite sphere	169:225	crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs)	169:235	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	1	11	link	crosslinked	169:179	arg1	CS/GNPs					228:234	CS/GNPs	228:234	CS/GNPs	228:234	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	1	11	link	crosslinked	169:179	arg1	sphere					220:225	crosslinked chitosan/graphene nanoplates composite sphere	169:225	crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs)	169:235	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	4	12	theme	CS/GNPs	664:670	arg1	reusability					649:659	the reusability	645:659	the reusability of CS/GNPs	645:670	In view of practical, the reusability of CS/GNPs was also tested.					
29447965	1	13	theme	nanoplates	199:208	arg1	CS/GNPs					228:234	CS/GNPs	228:234	CS/GNPs	228:234	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	1	13	theme	nanoplates	199:208	arg1	sphere					220:225	crosslinked chitosan/graphene nanoplates composite sphere	169:225	crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs)	169:235	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	0	14	theme	Simple	0:5	arg1	fabrication					7:17	Simple fabrication	0:17	Simple fabrication of Chitosan/Graphene	0:38	Simple fabrication of Chitosan/Graphene nanoplates composite spheres for efficient adsorption of acid dyes from aqueous solution.					
29447965	1	15	theme	composite	210:218	arg1	CS/GNPs					228:234	CS/GNPs	228:234	CS/GNPs	228:234	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	1	15	theme	composite	210:218	arg1	sphere					220:225	crosslinked chitosan/graphene nanoplates composite sphere	169:225	crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs)	169:235	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	0	16	theme	Chitosan/Graphene	22:38	arg1	fabrication					7:17	Simple fabrication	0:17	Simple fabrication of Chitosan/Graphene	0:38	Simple fabrication of Chitosan/Graphene nanoplates composite spheres for efficient adsorption of acid dyes from aqueous solution.					
29447965	1	17	theme	sphere	220:225	arg1	fabrication					154:164	the fabrication	150:164	the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs)	150:235	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	3	18	theme	red	507:509	arg1	AR1					514:516	AR1	514:516	AR1	514:516	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	3	18	theme	red	507:509	arg1	acid					502:505	acid red 1	502:511	acid red 1 (AR1)	502:517	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	3	19	theme	CS/GNPs	467:473	arg1	activities					453:462	The adsorption activities	438:462	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1)	438:517	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	0	20	theme	composite	51:59	arg1	spheres					61:67	composite spheres	51:67	composite spheres	51:67	Simple fabrication of Chitosan/Graphene nanoplates composite spheres for efficient adsorption of acid dyes from aqueous solution.					
29447965	3	21	theme	adsorption	442:451	arg1	activities					453:462	The adsorption activities	438:462	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1)	438:517	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	2	22	theme	thermogravimetric	404:420	arg1	TGA					432:434	TGA	432:434	TGA	432:434	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	2	22	theme	thermogravimetric	404:420	arg1	analysis					422:429	thermogravimetric analysis	404:429	thermogravimetric analysis (TGA)	404:435	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	6	23	theme	uptake	874:879	arg1	loss					881:884	little uptake loss	867:884	little uptake loss	867:884	After saturated adsorption, CS/GNPs can be efficiently regenerated and reused with little uptake loss.					
29447965	6	24	theme	little	867:872	arg1	loss					881:884	little uptake loss	867:884	little uptake loss	867:884	After saturated adsorption, CS/GNPs can be efficiently regenerated and reused with little uptake loss.					
29447965	0	25	from	solution	120:127	arg1	adsorption					83:92	efficient adsorption	73:92	efficient adsorption of acid dyes from aqueous solution	73:127	Simple fabrication of Chitosan/Graphene nanoplates composite spheres for efficient adsorption of acid dyes from aqueous solution.					
29447965	4	26	theme	practical	634:642	arg1	view					626:629	view	626:629	view of practical	626:642	In view of practical, the reusability of CS/GNPs was also tested.					
29447965	3	27	theme	GNPs	563:566	arg1	content					568:574	GNPs content	563:574	GNPs content	563:574	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	1	28	theme	facile	132:137	arg1	method					139:144	A facile method	130:144	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs)	130:235	A facile method for the fabrication of crosslinked chitosan/graphene nanoplates composite sphere (CS/GNPs) was presented.					
29447965	3	29	theme	adsorption	588:597	arg1	kinetics					599:606	adsorption kinetics	588:606	adsorption kinetics	588:606	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	3	30	theme	content	568:574	arg1	effect					546:551	the effect	542:551	the effect of pH and GNPs content	542:574	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	2	31	theme	X-ray	335:339	arg1	XRD					354:356	XRD	354:356	XRD	354:356	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	2	31	theme	X-ray	335:339	arg1	diffraction					341:351	X-ray diffraction	335:351	X-ray diffraction (XRD)	335:357	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	6	32	theme	saturated	790:798	arg1	adsorption					800:809	saturated adsorption	790:809	saturated adsorption	790:809	After saturated adsorption, CS/GNPs can be efficiently regenerated and reused with little uptake loss.					
29447965	3	33	theme	pH	556:557	arg1	effect					546:551	the effect	542:551	the effect of pH and GNPs content	542:574	The adsorption activities of CS/GNPs for methyl orange (MO) and acid red 1 (AR1) were evaluated such as the effect of pH and GNPs content, as well as adsorption kinetics and isotherms.					
29447965	7	34	theme	adsorbent	924:932	arg1	apromising					913:922	the apromising	909:922	the apromising adsorbent with non-toxic, efficient, low-cost and easy	909:977	Therefore, CS/GNPs is the apromising adsorbent with non-toxic, efficient, low-cost and easy to prepare for the dye removal.					
29447965	7	34	theme	adsorbent	924:932	arg1	CS/GNPs					898:904	CS/GNPs	898:904	CS/GNPs	898:904	Therefore, CS/GNPs is the apromising adsorbent with non-toxic, efficient, low-cost and easy to prepare for the dye removal.					
29447965	2	35	theme	photoelectron	366:378	arg1	XPS					395:397	XPS	395:397	XPS	395:397	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	2	35	theme	photoelectron	366:378	arg1	spectroscopic					380:392	X-ray photoelectron spectroscopic	360:392	X-ray photoelectron spectroscopic (XPS)	360:398	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	0	36	theme	efficient	73:81	arg1	adsorption					83:92	efficient adsorption	73:92	efficient adsorption of acid dyes from aqueous solution	73:127	Simple fabrication of Chitosan/Graphene nanoplates composite spheres for efficient adsorption of acid dyes from aqueous solution.					
29447965	2	37	theme	obtained	256:263	arg1	samples					265:271	The obtained samples	252:271	The obtained samples	252:271	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	2	38	theme	X-ray	360:364	arg1	XPS					395:397	XPS	395:397	XPS	395:397	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
29447965	2	38	theme	X-ray	360:364	arg1	spectroscopic					380:392	X-ray photoelectron spectroscopic	360:392	X-ray photoelectron spectroscopic (XPS)	360:398	The obtained samples were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopic (XPS) and thermogravimetric analysis (TGA).					
31550181	8	0	theme	sustained	929:937	arg1	GIR					962:964	GIR	962:964	GIR	962:964	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	8	0	theme	sustained	929:937	arg1	rate					956:959	sustained glucose infusion rate	929:959	sustained glucose infusion rate (GIR)	929:965	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	4	1	theme	high-fat	555:562	arg1	diet					564:567	60% high-fat diet	551:567	60% high-fat diet	551:567	Global CD44-deficient (cd44) mice and wild-type littermates (cd44) were fed a chow diet or 60% high-fat diet for 16 wk.					
31550181	11	2	theme	high-fat-fed	1336:1347	arg1	mice					1354:1357	high-fat-fed cd44 mice	1336:1357	high-fat-fed cd44 mice	1336:1357	Ameliorated muscle IR in high-fat-fed cd44 mice was associated with increased vascularization.					
31550181	12	3	theme	observed	1432:1439	arg1	increases					1441:1449	previously observed increases	1421:1449	previously observed increases in wild-type mice	1421:1467	In contrast to previously observed increases in wild-type mice, PEGPH20 treatment in high-fat-fed cd44 mice did not change GIR or muscle Rg during ICv, suggesting a CD44-dependent action.					
31550181	8	4	theme	infusion	947:954	arg1	GIR					962:964	GIR	962:964	GIR	962:964	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	8	4	theme	infusion	947:954	arg1	rate					956:959	sustained glucose infusion rate	929:959	sustained glucose infusion rate (GIR)	929:965	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	10	5	from	genotypes	1181:1189	arg1	diet					1199:1202	chow diet	1194:1202	chow diet	1194:1202	Moreover, gastrocnemius Rg remained unchanged between genotypes on chow diet but was increased in high-fat-fed cd44 compared with cd44 when normalized to clamp insulin concentrations.					
31550181	11	6	theme	increased	1379:1387	arg1	vascularization					1389:1403	increased vascularization	1379:1403	increased vascularization	1379:1403	Ameliorated muscle IR in high-fat-fed cd44 mice was associated with increased vascularization.					
31550181	10	7	theme	gastrocnemius	1137:1149	arg1	Rg					1151:1152	gastrocnemius Rg	1137:1152	gastrocnemius Rg	1137:1152	Moreover, gastrocnemius Rg remained unchanged between genotypes on chow diet but was increased in high-fat-fed cd44 compared with cd44 when normalized to clamp insulin concentrations.					
31550181	1	8	theme	mice	206:209	arg1	muscle					165:170	skeletal muscle	156:170	skeletal muscle of high-fat-fed insulin-resistant mice	156:209	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	8	9	from	weight	847:852	arg1	absence					816:822	the absence	812:822	the absence of differences in body weight and composition	812:868	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	2	10	theme	hyaluronan	330:339	arg1	CD44					315:318	CD44	315:318	CD44	315:318	CD44, the main hyaluronan receptor, is positively correlated with type 2 diabetes.					
31550181	2	10	theme	hyaluronan	330:339	arg1	receptor					341:348	the main hyaluronan receptor	321:348	the main hyaluronan receptor	321:348	CD44, the main hyaluronan receptor, is positively correlated with type 2 diabetes.					
31550181	12	11	from	treatment	1478:1486	arg1	mice					1509:1512	high-fat-fed cd44 mice	1491:1512	high-fat-fed cd44 mice	1491:1512	In contrast to previously observed increases in wild-type mice, PEGPH20 treatment in high-fat-fed cd44 mice did not change GIR or muscle Rg during ICv, suggesting a CD44-dependent action.					
31550181	12	12	theme	CD44-dependent	1571:1584	arg1	action					1586:1591	a CD44-dependent action	1569:1591	a CD44-dependent action	1569:1591	In contrast to previously observed increases in wild-type mice, PEGPH20 treatment in high-fat-fed cd44 mice did not change GIR or muscle Rg during ICv, suggesting a CD44-dependent action.					
31550181	4	13	theme	CD44-deficient	467:480	arg1	mice					489:492	Global CD44-deficient (cd44) mice	460:492	Global CD44-deficient (cd44) mice	460:492	Global CD44-deficient (cd44) mice and wild-type littermates (cd44) were fed a chow diet or 60% high-fat diet for 16 wk.					
31550181	8	14	from	composition	858:868	arg1	absence					816:822	the absence	812:822	the absence of differences in body weight and composition	812:868	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	11	15	theme	Ameliorated	1311:1321	arg1	IR					1330:1331	Ameliorated muscle IR	1311:1331	Ameliorated muscle IR in high-fat-fed cd44 mice	1311:1357	Ameliorated muscle IR in high-fat-fed cd44 mice was associated with increased vascularization.					
31550181	9	16	theme	High-fat	987:994	arg1	muscle					1009:1014	High-fat diet-induced muscle	987:1014	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg)	987:1070	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	4	17	theme	wild-type	498:506	arg1	littermates					508:518	wild-type littermates	498:518	wild-type littermates (cd44)	498:525	Global CD44-deficient (cd44) mice and wild-type littermates (cd44) were fed a chow diet or 60% high-fat diet for 16 wk.					
31550181	4	17	theme	wild-type	498:506	arg1	cd44					521:524	cd44	521:524	cd44	521:524	Global CD44-deficient (cd44) mice and wild-type littermates (cd44) were fed a chow diet or 60% high-fat diet for 16 wk.					
31550181	9	18	from	mice	1121:1124	arg1	absent					1106:1111	absent	1106:1111	absent	1106:1111	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	0	19	theme	high-fat-fed	81:92	arg1	mice					102:105	high-fat-fed C57BL/6 mice	81:105	high-fat-fed C57BL/6 mice	81:105	CD44 contributes to hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 mice.					
31550181	1	20	theme	high-fat-fed	175:186	arg1	mice					206:209	high-fat-fed insulin-resistant mice	175:209	high-fat-fed insulin-resistant mice	175:209	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	12	21	theme	cd44	1504:1507	arg1	mice					1509:1512	high-fat-fed cd44 mice	1491:1512	high-fat-fed cd44 mice	1491:1512	In contrast to previously observed increases in wild-type mice, PEGPH20 treatment in high-fat-fed cd44 mice did not change GIR or muscle Rg during ICv, suggesting a CD44-dependent action.					
31550181	0	22	theme	mice	102:105	arg1	muscle					71:76	skeletal muscle	62:76	skeletal muscle of high-fat-fed C57BL/6 mice	62:105	CD44 contributes to hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 mice.					
31550181	10	23	dep	increased	1212:1220	arg1	compared					1243:1250	compared	1243:1250	compared with cd44	1243:1260	Moreover, gastrocnemius Rg remained unchanged between genotypes on chow diet but was increased in high-fat-fed cd44 compared with cd44 when normalized to clamp insulin concentrations.					
31550181	4	24	theme	cd44	483:486	arg1	mice					489:492	Global CD44-deficient (cd44) mice	460:492	Global CD44-deficient (cd44) mice	460:492	Global CD44-deficient (cd44) mice and wild-type littermates (cd44) were fed a chow diet or 60% high-fat diet for 16 wk.					
31550181	13	25	theme	muscle	1640:1645	arg1	IR					1647:1648	muscle IR	1640:1648	muscle IR	1640:1648	In conclusion, genetic CD44 deletion improves muscle IR, and the beneficial effects of PEGPH20 are CD44-dependent.					
31550181	9	26	theme	muscle	1045:1050	arg1	Rg					1068:1069	Rg	1068:1069	Rg	1068:1069	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	9	26	theme	muscle	1045:1050	arg1	uptake					1060:1065	decreased muscle glucose uptake	1035:1065	decreased muscle glucose uptake (Rg)	1035:1070	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	14	27	theme	muscle	1788:1793	arg1	IR					1795:1796	hyaluronan-mediated muscle IR	1768:1796	hyaluronan-mediated muscle IR	1768:1796	These results suggest a critical role of CD44 in promoting hyaluronan-mediated muscle IR, therefore representing a potential therapeutic target for diabetes.					
31550181	8	28	theme	clamp	885:889	arg1	insulin					891:897	lower clamp insulin	879:897	lower clamp insulin during ICv	879:908	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	4	29	theme	chow	538:541	arg1	diet					543:546	a chow diet	536:546	a chow diet	536:546	Global CD44-deficient (cd44) mice and wild-type littermates (cd44) were fed a chow diet or 60% high-fat diet for 16 wk.					
31550181	13	30	theme	genetic	1609:1615	arg1	deletion					1622:1629	genetic CD44 deletion	1609:1629	genetic CD44 deletion	1609:1629	In conclusion, genetic CD44 deletion improves muscle IR, and the beneficial effects of PEGPH20 are CD44-dependent.					
31550181	7	31	theme	CD44	784:787	arg1	expression					797:806	muscle CD44 protein expression	777:806	muscle CD44 protein expression	777:806	High-fat feeding increased muscle CD44 protein expression.					
31550181	1	32	theme	matrix	122:127	arg1	hyaluronan					129:138	Extracellular matrix hyaluronan	108:138	Extracellular matrix hyaluronan	108:138	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	0	33	theme	insulin	40:46	arg1	resistance					48:57	hyaluronan-mediated insulin resistance	20:57	hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 mice	20:105	CD44 contributes to hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 mice.					
31550181	14	34	theme	CD44	1750:1753	arg1	role					1742:1745	a critical role	1731:1745	a critical role of CD44 in promoting hyaluronan-mediated muscle IR	1731:1796	These results suggest a critical role of CD44 in promoting hyaluronan-mediated muscle IR, therefore representing a potential therapeutic target for diabetes.					
31550181	12	35	theme	high-fat-fed	1491:1502	arg1	mice					1509:1512	high-fat-fed cd44 mice	1491:1512	high-fat-fed cd44 mice	1491:1512	In contrast to previously observed increases in wild-type mice, PEGPH20 treatment in high-fat-fed cd44 mice did not change GIR or muscle Rg during ICv, suggesting a CD44-dependent action.					
31550181	10	36	theme	insulin	1287:1293	arg1	concentrations					1295:1308	clamp insulin concentrations	1281:1308	clamp insulin concentrations	1281:1308	Moreover, gastrocnemius Rg remained unchanged between genotypes on chow diet but was increased in high-fat-fed cd44 compared with cd44 when normalized to clamp insulin concentrations.					
31550181	7	37	theme	High-fat	750:757	arg1	feeding					759:765	High-fat feeding	750:765	High-fat feeding	750:765	High-fat feeding increased muscle CD44 protein expression.					
31550181	12	38	theme	wild-type	1454:1462	arg1	mice					1464:1467	wild-type mice	1454:1467	wild-type mice	1454:1467	In contrast to previously observed increases in wild-type mice, PEGPH20 treatment in high-fat-fed cd44 mice did not change GIR or muscle Rg during ICv, suggesting a CD44-dependent action.					
31550181	8	39	theme	cd44	915:918	arg1	mice					920:923	the cd44 mice	911:923	the cd44 mice	911:923	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	11	40	theme	cd44	1349:1352	arg1	mice					1354:1357	high-fat-fed cd44 mice	1336:1357	high-fat-fed cd44 mice	1336:1357	Ameliorated muscle IR in high-fat-fed cd44 mice was associated with increased vascularization.					
31550181	6	41	theme	hyperinsulinemic-euglycemic	709:735	arg1	ICv					744:746	ICv	744:746	ICv	744:746	Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp (ICv).					
31550181	6	41	theme	hyperinsulinemic-euglycemic	709:735	arg1	clamp					737:741	hyperinsulinemic-euglycemic clamp	709:741	hyperinsulinemic-euglycemic clamp (ICv)	709:747	Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp (ICv).					
31550181	14	42	dep	potential	1824:1832	arg1	therapeutic					1834:1844	therapeutic	1834:1844	therapeutic	1834:1844	These results suggest a critical role of CD44 in promoting hyaluronan-mediated muscle IR, therefore representing a potential therapeutic target for diabetes.					
31550181	8	43	theme	glucose	939:945	arg1	GIR					962:964	GIR	962:964	GIR	962:964	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	8	43	theme	glucose	939:945	arg1	rate					956:959	sustained glucose infusion rate	929:959	sustained glucose infusion rate (GIR)	929:965	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	9	44	theme	cd44	1116:1119	arg1	mice					1121:1124	cd44 mice	1116:1124	cd44 mice	1116:1124	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	2	45	theme	type	381:384	arg1	diabetes					388:395	type 2 diabetes	381:395	type 2 diabetes	381:395	CD44, the main hyaluronan receptor, is positively correlated with type 2 diabetes.					
31550181	1	46	theme	insulin	290:296	arg1	resistance					298:307	diet-induced insulin resistance	277:307	diet-induced insulin resistance (IR)	277:312	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	1	46	theme	insulin	290:296	arg1	IR					310:311	IR	310:311	IR	310:311	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	4	47	theme	%	553:553	arg1	diet					564:567	60% high-fat diet	551:567	60% high-fat diet	551:567	Global CD44-deficient (cd44) mice and wild-type littermates (cd44) were fed a chow diet or 60% high-fat diet for 16 wk.					
31550181	8	48	from	absence	816:822	arg1	composition					858:868	composition	858:868	composition	858:868	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	8	48	from	absence	816:822	arg1	weight					847:852	body weight	842:852	body weight	842:852	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	5	49	theme	cd44	593:596	arg1	mice					598:601	High-fat-fed cd44 mice	580:601	High-fat-fed cd44 mice	580:601	High-fat-fed cd44 mice were also treated with PEGPH20 to evaluate its CD44-dependent action.					
31550181	1	50	theme	insulin-resistant	188:204	arg1	mice					206:209	high-fat-fed insulin-resistant mice	175:209	high-fat-fed insulin-resistant mice	175:209	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	9	51	from	absent	1106:1111	arg1	mice					1121:1124	cd44 mice	1116:1124	cd44 mice	1116:1124	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	2	52	theme	main	325:328	arg1	CD44					315:318	CD44	315:318	CD44	315:318	CD44, the main hyaluronan receptor, is positively correlated with type 2 diabetes.					
31550181	2	52	theme	main	325:328	arg1	receptor					341:348	the main hyaluronan receptor	321:348	the main hyaluronan receptor	321:348	CD44, the main hyaluronan receptor, is positively correlated with type 2 diabetes.					
31550181	9	53	theme	cd44+/+	1089:1095	arg1	mice					1097:1100	cd44+/+ mice	1089:1100	cd44+/+ mice	1089:1100	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	4	54	theme	Global	460:465	arg1	mice					489:492	Global CD44-deficient (cd44) mice	460:492	Global CD44-deficient (cd44) mice	460:492	Global CD44-deficient (cd44) mice and wild-type littermates (cd44) were fed a chow diet or 60% high-fat diet for 16 wk.					
31550181	8	55	from	differences	827:837	arg1	composition					858:868	composition	858:868	composition	858:868	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	8	55	from	differences	827:837	arg1	weight					847:852	body weight	842:852	body weight	842:852	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	13	56	theme	PEGPH20	1681:1687	arg1	CD44-dependent					1693:1706	CD44-dependent	1693:1706	CD44-dependent	1693:1706	In conclusion, genetic CD44 deletion improves muscle IR, and the beneficial effects of PEGPH20 are CD44-dependent.					
31550181	13	56	theme	PEGPH20	1681:1687	arg1	effects					1670:1676	the beneficial effects	1655:1676	the beneficial effects of PEGPH20	1655:1687	In conclusion, genetic CD44 deletion improves muscle IR, and the beneficial effects of PEGPH20 are CD44-dependent.					
31550181	8	57	contain	had	925:927	arg2	GIR					962:964	GIR	962:964	GIR	962:964	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	8	57	contain	had	925:927	arg2	rate					956:959	sustained glucose infusion rate	929:959	sustained glucose infusion rate (GIR)	929:965	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	8	57	contain	had	925:927	arg1	mice					920:923	the cd44 mice	911:923	the cd44 mice	911:923	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	1	58	theme	hyaluronan	229:238	arg1	reduction					216:224	reduction	216:224	reduction of hyaluronan by PEGPH20 hyaluronidase	216:263	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	10	59	theme	chow	1194:1197	arg1	diet					1199:1202	chow diet	1194:1202	chow diet	1194:1202	Moreover, gastrocnemius Rg remained unchanged between genotypes on chow diet but was increased in high-fat-fed cd44 compared with cd44 when normalized to clamp insulin concentrations.					
31550181	5	60	theme	CD44-dependent	650:663	arg1	action					665:670	its CD44-dependent action	646:670	its CD44-dependent action	646:670	High-fat-fed cd44 mice were also treated with PEGPH20 to evaluate its CD44-dependent action.					
31550181	14	61	theme	potential	1824:1832	arg1	target					1846:1851	a potential therapeutic target	1822:1851	a potential therapeutic target for diabetes	1822:1864	These results suggest a critical role of CD44 in promoting hyaluronan-mediated muscle IR, therefore representing a potential therapeutic target for diabetes.					
31550181	9	62	theme	diet-induced	996:1007	arg1	muscle					1009:1014	High-fat diet-induced muscle	987:1014	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg)	987:1070	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	1	63	theme	skeletal	156:163	arg1	muscle					165:170	skeletal muscle	156:170	skeletal muscle of high-fat-fed insulin-resistant mice	156:209	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	12	64	theme	muscle	1536:1541	arg1	Rg					1543:1544	muscle Rg	1536:1544	muscle Rg	1536:1544	In contrast to previously observed increases in wild-type mice, PEGPH20 treatment in high-fat-fed cd44 mice did not change GIR or muscle Rg during ICv, suggesting a CD44-dependent action.					
31550181	0	65	theme	C57BL/6	94:100	arg1	mice					102:105	high-fat-fed C57BL/6 mice	81:105	high-fat-fed C57BL/6 mice	81:105	CD44 contributes to hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 mice.					
31550181	3	66	from	role	424:427	arg1	muscle					449:454	skeletal muscle IR	440:457	skeletal muscle IR	440:457	This study determines the role of CD44 in skeletal muscle IR.					
31550181	8	67	theme	differences	827:837	arg1	absence					816:822	the absence	812:822	the absence of differences in body weight and composition	812:868	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	13	68	theme	beneficial	1659:1668	arg1	CD44-dependent					1693:1706	CD44-dependent	1693:1706	CD44-dependent	1693:1706	In conclusion, genetic CD44 deletion improves muscle IR, and the beneficial effects of PEGPH20 are CD44-dependent.					
31550181	13	68	theme	beneficial	1659:1668	arg1	effects					1670:1676	the beneficial effects	1655:1676	the beneficial effects of PEGPH20	1655:1687	In conclusion, genetic CD44 deletion improves muscle IR, and the beneficial effects of PEGPH20 are CD44-dependent.					
31550181	11	69	from	IR	1330:1331	arg1	mice					1354:1357	high-fat-fed cd44 mice	1336:1357	high-fat-fed cd44 mice	1336:1357	Ameliorated muscle IR in high-fat-fed cd44 mice was associated with increased vascularization.					
31550181	8	70	theme	body	842:845	arg1	weight					847:852	body weight	842:852	body weight	842:852	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	9	71	theme	decreased	1035:1043	arg1	Rg					1068:1069	Rg	1068:1069	Rg	1068:1069	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	9	71	theme	decreased	1035:1043	arg1	uptake					1060:1065	decreased muscle glucose uptake	1035:1065	decreased muscle glucose uptake (Rg)	1035:1070	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	5	72	theme	High-fat-fed	580:591	arg1	mice					598:601	High-fat-fed cd44 mice	580:601	High-fat-fed cd44 mice	580:601	High-fat-fed cd44 mice were also treated with PEGPH20 to evaluate its CD44-dependent action.					
31550181	0	73	from	resistance	48:57	arg1	muscle					71:76	skeletal muscle	62:76	skeletal muscle of high-fat-fed C57BL/6 mice	62:105	CD44 contributes to hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 mice.					
31550181	11	74	theme	muscle	1323:1328	arg1	IR					1330:1331	Ameliorated muscle IR	1311:1331	Ameliorated muscle IR in high-fat-fed cd44 mice	1311:1357	Ameliorated muscle IR in high-fat-fed cd44 mice was associated with increased vascularization.					
31550181	6	75	theme	Insulin	673:679	arg1	sensitivity					681:691	Insulin sensitivity	673:691	Insulin sensitivity	673:691	Insulin sensitivity was measured by hyperinsulinemic-euglycemic clamp (ICv).					
31550181	9	76	theme	glucose	1052:1058	arg1	Rg					1068:1069	Rg	1068:1069	Rg	1068:1069	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	9	76	theme	glucose	1052:1058	arg1	uptake					1060:1065	decreased muscle glucose uptake	1035:1065	decreased muscle glucose uptake (Rg)	1035:1070	High-fat diet-induced muscle IR as evidenced by decreased muscle glucose uptake (Rg) was exhibited in cd44+/+ mice but absent in cd44 mice.					
31550181	3	77	theme	skeletal	440:447	arg1	muscle					449:454	skeletal muscle IR	440:457	skeletal muscle IR	440:457	This study determines the role of CD44 in skeletal muscle IR.					
31550181	0	78	theme	hyaluronan-mediated	20:38	arg1	resistance					48:57	hyaluronan-mediated insulin resistance	20:57	hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 mice	20:105	CD44 contributes to hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 mice.					
31550181	14	79	theme	hyaluronan-mediated	1768:1786	arg1	IR					1795:1796	hyaluronan-mediated muscle IR	1768:1796	hyaluronan-mediated muscle IR	1768:1796	These results suggest a critical role of CD44 in promoting hyaluronan-mediated muscle IR, therefore representing a potential therapeutic target for diabetes.					
31550181	1	80	theme	Extracellular	108:120	arg1	hyaluronan					129:138	Extracellular matrix hyaluronan	108:138	Extracellular matrix hyaluronan	108:138	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	8	81	theme	lower	879:883	arg1	insulin					891:897	lower clamp insulin	879:897	lower clamp insulin during ICv	879:908	In the absence of differences in body weight and composition, despite lower clamp insulin during ICv, the cd44 mice had sustained glucose infusion rate (GIR) regardless of diet.					
31550181	4	82	theme	60	551:552	arg1	%					553:553	%	553:553	%	553:553	Global CD44-deficient (cd44) mice and wild-type littermates (cd44) were fed a chow diet or 60% high-fat diet for 16 wk.					
31550181	13	83	theme	CD44	1617:1620	arg1	deletion					1622:1629	genetic CD44 deletion	1609:1629	genetic CD44 deletion	1609:1629	In conclusion, genetic CD44 deletion improves muscle IR, and the beneficial effects of PEGPH20 are CD44-dependent.					
31550181	7	84	theme	protein	789:795	arg1	expression					797:806	muscle CD44 protein expression	777:806	muscle CD44 protein expression	777:806	High-fat feeding increased muscle CD44 protein expression.					
31550181	12	85	theme	PEGPH20	1470:1476	arg1	treatment					1478:1486	PEGPH20 treatment	1470:1486	PEGPH20 treatment in high-fat-fed cd44 mice	1470:1512	In contrast to previously observed increases in wild-type mice, PEGPH20 treatment in high-fat-fed cd44 mice did not change GIR or muscle Rg during ICv, suggesting a CD44-dependent action.					
31550181	3	86	theme	CD44	432:435	arg1	role					424:427	the role	420:427	the role of CD44 in skeletal muscle IR	420:457	This study determines the role of CD44 in skeletal muscle IR.					
31550181	0	87	theme	skeletal	62:69	arg1	muscle					71:76	skeletal muscle	62:76	skeletal muscle of high-fat-fed C57BL/6 mice	62:105	CD44 contributes to hyaluronan-mediated insulin resistance in skeletal muscle of high-fat-fed C57BL/6 mice.					
31550181	7	88	theme	muscle	777:782	arg1	expression					797:806	muscle CD44 protein expression	777:806	muscle CD44 protein expression	777:806	High-fat feeding increased muscle CD44 protein expression.					
31550181	10	89	theme	clamp	1281:1285	arg1	concentrations					1295:1308	clamp insulin concentrations	1281:1308	clamp insulin concentrations	1281:1308	Moreover, gastrocnemius Rg remained unchanged between genotypes on chow diet but was increased in high-fat-fed cd44 compared with cd44 when normalized to clamp insulin concentrations.					
31550181	14	90	theme	critical	1733:1740	arg1	role					1742:1745	a critical role	1731:1745	a critical role of CD44 in promoting hyaluronan-mediated muscle IR	1731:1796	These results suggest a critical role of CD44 in promoting hyaluronan-mediated muscle IR, therefore representing a potential therapeutic target for diabetes.					
31550181	10	91	theme	high-fat-fed	1225:1236	arg1	cd44					1238:1241	high-fat-fed cd44	1225:1241	high-fat-fed cd44	1225:1241	Moreover, gastrocnemius Rg remained unchanged between genotypes on chow diet but was increased in high-fat-fed cd44 compared with cd44 when normalized to clamp insulin concentrations.					
31550181	12	92	from	increases	1441:1449	arg1	mice					1464:1467	wild-type mice	1454:1467	wild-type mice	1454:1467	In contrast to previously observed increases in wild-type mice, PEGPH20 treatment in high-fat-fed cd44 mice did not change GIR or muscle Rg during ICv, suggesting a CD44-dependent action.					
31550181	1	93	theme	diet-induced	277:288	arg1	resistance					298:307	diet-induced insulin resistance	277:307	diet-induced insulin resistance (IR)	277:312	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
31550181	1	93	theme	diet-induced	277:288	arg1	IR					310:311	IR	310:311	IR	310:311	Extracellular matrix hyaluronan is increased in skeletal muscle of high-fat-fed insulin-resistant mice, and reduction of hyaluronan by PEGPH20 hyaluronidase ameliorates diet-induced insulin resistance (IR).					
30827670	4	0	theme	relative	700:707	arg1	DPPH					709:712	relative DPPH	700:712	relative DPPH	700:712	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	6	1	theme	chrysanthemum	1157:1169	arg1	tea					1187:1189	a functional tea	1174:1189	a functional tea	1174:1189	The results from this research may be used to promote the consumption of chrysanthemum as a functional tea.					
30827670	6	1	theme	chrysanthemum	1157:1169	arg1	consumption					1142:1152	the consumption	1138:1152	the consumption of chrysanthemum	1138:1169	The results from this research may be used to promote the consumption of chrysanthemum as a functional tea.					
30827670	5	2	from	production	1054:1063	arg1	cells					1077:1081	cultured cells	1068:1081	cultured cells	1068:1081	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	1	3	theme	methanol	257:264	arg1	extracts					266:273	the 75% methanol extracts	249:273	the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis	249:334	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	5	4	theme	cyclooxygenase-2	964:979	arg1	expressions					986:996	IL-1β and cyclooxygenase-2 mRNA expressions	954:996	IL-1β and cyclooxygenase-2 mRNA expressions	954:996	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	4	5	theme	DPPH	709:712	arg1	values					753:758	relative DPPH and oxygen radical absorbance capacity values	700:758	relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g	700:842	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	5	6	theme	interleukin-6	939:951	arg1	production					1054:1063	the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production	908:1063	the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells	908:1081	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	5	7	theme	intracellular	1016:1028	arg1	species					1046:1052	H2O2-induced intracellular reactive oxygen species	1003:1052	H2O2-induced intracellular reactive oxygen species	1003:1052	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	4	8	theme	acid	779:782	arg1	values					753:758	relative DPPH and oxygen radical absorbance capacity values	700:758	relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g	700:842	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	2	9	located	detected	476:483	arg1	HuaiJu					507:512	HuaiJu	507:512	HuaiJu	507:512	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	2	9	located	detected	476:483	arg1	GongJu					496:501	GongJu	496:501	GongJu	496:501	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	2	9	located	detected	476:483	arg1	HangJu					488:493	HangJu	488:493	HangJu	488:493	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	2	9	located	detected	476:483	arg2	acetylmarein					459:470	acetylmarein	459:470	acetylmarein	459:470	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	3	10	theme	radical	557:563	arg1	scavenging					565:574	their radical scavenging	551:574	their radical scavenging	551:574	The extracts were also examined for their radical scavenging and anti-inflammatory activities in vitro.					
30827670	4	11	theme	absorbance	733:742	arg1	capacity					744:751	oxygen radical absorbance capacity	718:751	oxygen radical absorbance capacity	718:751	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	6	12	used	used	1122:1125	arg2	results					1088:1094	The results	1084:1094	The results from this research	1084:1113	The results from this research may be used to promote the consumption of chrysanthemum as a functional tea.					
30827670	4	13	theme	105.48	799:804	arg1	values					753:758	relative DPPH and oxygen radical absorbance capacity values	700:758	relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g	700:842	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	5	14	theme	mRNA	981:984	arg1	expressions					986:996	IL-1β and cyclooxygenase-2 mRNA expressions	954:996	IL-1β and cyclooxygenase-2 mRNA expressions	954:996	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	4	15	theme	phenolic	678:685	arg1	content					687:693	total phenolic content	672:693	the greatest total phenolic content	659:693	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	5	16	theme	IL-1β	954:958	arg1	expressions					986:996	IL-1β and cyclooxygenase-2 mRNA expressions	954:996	IL-1β and cyclooxygenase-2 mRNA expressions	954:996	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	4	17	theme	total	672:676	arg1	content					687:693	total phenolic content	672:693	the greatest total phenolic content	659:693	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	6	18	from	research	1106:1113	arg1	results					1088:1094	The results	1084:1094	The results from this research	1084:1113	The results from this research may be used to promote the consumption of chrysanthemum as a functional tea.					
30827670	3	19	theme	anti-inflammatory	580:596	arg1	activities					598:607	anti-inflammatory activities	580:607	anti-inflammatory activities	580:607	The extracts were also examined for their radical scavenging and anti-inflammatory activities in vitro.					
30827670	0	20	theme	Chemical	0:7	arg1	compositions					9:20	Chemical compositions	0:20	Chemical compositions of chrysanthemum teas	0:42	Chemical compositions of chrysanthemum teas and their anti-inflammatory and antioxidant properties.					
30827670	1	21	theme	chemical	285:292	arg1	compositions					294:305	their chemical compositions	279:305	their chemical compositions using UPLC/Q-TOF-MS analysis	279:334	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	5	22	theme	hot-H2O	880:886	arg1	extracts					888:895	all the hot-H2O extracts	872:895	all the hot-H2O extracts	872:895	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	1	23	theme	commercial	110:119	arg1	morifolium					155:164	Chrysanthemum morifolium	141:164	Chrysanthemum morifolium	141:164	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	23	theme	commercial	110:119	arg1	tinctoria					180:188	Coreopsis tinctoria	170:188	Coreopsis tinctoria	170:188	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	23	theme	commercial	110:119	arg1	teas					135:138	Seventeen commercial chrysanthemum teas	100:138	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria)	100:189	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	0	24	theme	chrysanthemum	25:37	arg1	teas					39:42	chrysanthemum teas	25:42	chrysanthemum teas	25:42	Chemical compositions of chrysanthemum teas and their anti-inflammatory and antioxidant properties.					
30827670	5	25	theme	expressions	986:996	arg1	production					1054:1063	the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production	908:1063	the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells	908:1081	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	1	26	theme	chrysanthemum	121:133	arg1	morifolium					155:164	Chrysanthemum morifolium	141:164	Chrysanthemum morifolium	141:164	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	26	theme	chrysanthemum	121:133	arg1	tinctoria					180:188	Coreopsis tinctoria	170:188	Coreopsis tinctoria	170:188	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	26	theme	chrysanthemum	121:133	arg1	teas					135:138	Seventeen commercial chrysanthemum teas	100:138	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria)	100:189	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	5	27	theme	reactive	1030:1037	arg1	species					1046:1052	H2O2-induced intracellular reactive oxygen species	1003:1052	H2O2-induced intracellular reactive oxygen species	1003:1052	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	6	28	theme	functional	1176:1185	arg1	tea					1187:1189	a functional tea	1174:1189	a functional tea	1174:1189	The results from this research may be used to promote the consumption of chrysanthemum as a functional tea.					
30827670	6	28	theme	functional	1176:1185	arg1	consumption					1142:1152	the consumption	1138:1152	the consumption of chrysanthemum	1138:1169	The results from this research may be used to promote the consumption of chrysanthemum as a functional tea.					
30827670	5	29	theme	species	1046:1052	arg1	production					1054:1063	the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production	908:1063	the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells	908:1081	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	4	30	theme	12.72 mg	763:770	arg1	acid					779:782	12.72 mg gallic acid equivalents/g	763:796	12.72 mg gallic acid equivalents/g	763:796	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	2	31	theme	Snow	435:438	arg1	chrysanthemum					440:452	the Snow chrysanthemum	431:452	the Snow chrysanthemum	431:452	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	1	32	theme	UPLC/Q-TOF-MS	313:325	arg1	analysis					327:334	UPLC/Q-TOF-MS analysis	313:334	UPLC/Q-TOF-MS analysis	313:334	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	4	33	theme	greatest	663:670	arg1	content					687:693	total phenolic content	672:693	the greatest total phenolic content	659:693	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	0	34	theme	teas	39:42	arg1	properties					88:97	their anti-inflammatory and antioxidant properties	48:97	their anti-inflammatory and antioxidant properties	48:97	Chemical compositions of chrysanthemum teas and their anti-inflammatory and antioxidant properties.					
30827670	0	34	theme	teas	39:42	arg1	compositions					9:20	Chemical compositions	0:20	Chemical compositions of chrysanthemum teas	0:42	Chemical compositions of chrysanthemum teas and their anti-inflammatory and antioxidant properties.					
30827670	4	35	theme	oxygen	718:723	arg1	capacity					744:751	oxygen radical absorbance capacity	718:751	oxygen radical absorbance capacity	718:751	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	4	36	theme	Kunlunmiju	642:651	arg1	extract					631:637	The hot-H2O extract	619:637	The hot-H2O extract of Kunlunmiju 1	619:653	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	0	37	theme	anti-inflammatory	54:70	arg1	properties					88:97	their anti-inflammatory and antioxidant properties	48:97	their anti-inflammatory and antioxidant properties	48:97	Chemical compositions of chrysanthemum teas and their anti-inflammatory and antioxidant properties.					
30827670	5	38	theme	lipopolysaccharide-induced	912:937	arg1	production					1054:1063	the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production	908:1063	the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells	908:1081	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	4	39	contain	had	655:657	arg1	extract					631:637	The hot-H2O extract	619:637	The hot-H2O extract of Kunlunmiju 1	619:653	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	4	39	contain	had	655:657	arg2	content					687:693	total phenolic content	672:693	the greatest total phenolic content	659:693	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	5	40	theme	oxygen	1039:1044	arg1	species					1046:1052	H2O2-induced intracellular reactive oxygen species	1003:1052	H2O2-induced intracellular reactive oxygen species	1003:1052	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	4	41	theme	radical	725:731	arg1	capacity					744:751	oxygen radical absorbance capacity	718:751	oxygen radical absorbance capacity	718:751	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	5	42	theme	H2O2-induced	1003:1014	arg1	species					1046:1052	H2O2-induced intracellular reactive oxygen species	1003:1052	H2O2-induced intracellular reactive oxygen species	1003:1052	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	1	43	theme	Chrysanthemum	141:153	arg1	morifolium					155:164	Chrysanthemum morifolium	141:164	Chrysanthemum morifolium	141:164	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	43	theme	Chrysanthemum	141:153	arg1	teas					135:138	Seventeen commercial chrysanthemum teas	100:138	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria)	100:189	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	4	44	dep	acid	779:782	arg1	equivalents/g					830:842	equivalents/g	830:842	equivalents/g	830:842	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	2	45	located	detected	419:426	arg2	C-diglucosylapigenin					364:383	C-diglucosylapigenin	364:383	C-diglucosylapigenin	364:383	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	2	45	located	detected	419:426	arg2	8-C					360:362	8-C	360:362	8-C	360:362	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	2	45	located	detected	419:426	arg2	6					357:357	6	357:357	6	357:357	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	2	45	located	detected	419:426	arg2	eriodicyol-7-O-glucoside					389:412	eriodicyol-7-O-glucoside	389:412	eriodicyol-7-O-glucoside	389:412	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	2	45	located	detected	419:426	arg1	chrysanthemum					440:452	the Snow chrysanthemum	431:452	the Snow chrysanthemum	431:452	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	4	46	theme	1222.50 μmol	810:821	arg1	Trolox					823:828	1222.50 μmol Trolox	810:828	1222.50 μmol Trolox	810:828	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	4	47	theme	capacity	744:751	arg1	values					753:758	relative DPPH and oxygen radical absorbance capacity values	700:758	relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g	700:842	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	4	48	theme	gallic	772:777	arg1	acid					779:782	12.72 mg gallic acid equivalents/g	763:796	12.72 mg gallic acid equivalents/g	763:796	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	5	49	theme	cultured	1068:1075	arg1	cells					1077:1081	cultured cells	1068:1081	cultured cells	1068:1081	In addition, all the hot-H2O extracts suppressed the lipopolysaccharide-induced interleukin-6, IL-1β and cyclooxygenase-2 mRNA expressions, and H2O2-induced intracellular reactive oxygen species production in cultured cells.					
30827670	2	50	theme	first	345:349	arg1	time					351:354	the first time	341:354	the first time	341:354	For the first time, 6, 8-C,C-diglucosylapigenin and eriodicyol-7-O-glucoside were detected in the Snow chrysanthemum, and acetylmarein was detected in HangJu, GongJu and HuaiJu.					
30827670	4	51	theme	hot-H2O	623:629	arg1	extract					631:637	The hot-H2O extract	619:637	The hot-H2O extract of Kunlunmiju 1	619:653	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	0	52	theme	antioxidant	76:86	arg1	properties					88:97	their anti-inflammatory and antioxidant properties	48:97	their anti-inflammatory and antioxidant properties	48:97	Chemical compositions of chrysanthemum teas and their anti-inflammatory and antioxidant properties.					
30827670	1	53	theme	Coreopsis	170:178	arg1	tinctoria					180:188	Coreopsis tinctoria	170:188	Coreopsis tinctoria	170:188	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	53	theme	Coreopsis	170:178	arg1	teas					135:138	Seventeen commercial chrysanthemum teas	100:138	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria)	100:189	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	54	theme	75	253:254	arg1	%					255:255	%	255:255	%	255:255	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	4	55	theme	Trolox	823:828	arg1	values					753:758	relative DPPH and oxygen radical absorbance capacity values	700:758	relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g	700:842	The hot-H2O extract of Kunlunmiju 1 had the greatest total phenolic content, and relative DPPH and oxygen radical absorbance capacity values of 12.72 mg gallic acid equivalents/g, 105.48 and 1222.50 μmol Trolox equivalents/g, respectively.					
30827670	1	56	dep	teas	135:138	arg1	morifolium					155:164	Chrysanthemum morifolium	141:164	Chrysanthemum morifolium	141:164	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	56	dep	teas	135:138	arg1	tinctoria					180:188	Coreopsis tinctoria	170:188	Coreopsis tinctoria	170:188	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	56	dep	teas	135:138	arg1	teas					135:138	Seventeen commercial chrysanthemum teas	100:138	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria)	100:189	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
30827670	1	57	theme	%	255:255	arg1	extracts					266:273	the 75% methanol extracts	249:273	the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis	249:334	Seventeen commercial chrysanthemum teas (Chrysanthemum morifolium and Coreopsis tinctoria) were extracted with hot-H2O, and examined and compared to the 75% methanol extracts for their chemical compositions using UPLC/Q-TOF-MS analysis.					
29914092	0	0	theme	Film	86:89	arg1	Composite					91:99	Bioactive Film Composite	76:99	Bioactive Film Composite	76:99	Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite.					
29914092	4	1	theme	various	568:574	arg1	type					576:579	the various type	564:579	the various type of collagen yield	564:597	Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film.					
29914092	1	2	theme	pepsin	142:147	arg1	PSC					171:173	PSC	171:173	PSC	171:173	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	1	2	theme	pepsin	142:147	arg1	collagen					161:168	pepsin solubilised collagen	142:168	pepsin solubilised collagen (PSC)	142:174	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	0	3	theme	Bioactive	76:84	arg1	Composite					91:99	Bioactive Film Composite	76:99	Bioactive Film Composite	76:99	Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite.					
29914092	1	4	theme	solubilised	149:159	arg1	PSC					171:173	PSC	171:173	PSC	171:173	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	1	4	theme	solubilised	149:159	arg1	collagen					161:168	pepsin solubilised collagen	142:168	pepsin solubilised collagen (PSC)	142:174	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	6	5	theme	interaction	941:951	arg1	occurrence					909:918	the occurrence	905:918	the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen	905:1018	Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen.					
29914092	3	6	dep	Fourier	450:456	arg1	Transform					458:466	Transform	458:466	Transform Infrared (FTIR) spectra analysis	458:499	As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure.					
29914092	6	7	theme	secondary	988:996	arg1	structure					998:1006	the secondary structure	984:1006	the secondary structure of collagen	984:1018	Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen.					
29914092	1	8	theme	cartilaginous	224:236	arg1	fish					238:241	a cartilaginous fish	222:241	a cartilaginous fish (Mustelus mustelus)	222:261	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	6	9	theme	collagen	1011:1018	arg1	structure					998:1006	the secondary structure	984:1006	the secondary structure of collagen	984:1018	Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen.					
29914092	2	10	dep	%	297:297	arg1	&beta					404:408	&beta	404:408	&beta	404:408	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	2	10	dep	%	297:297	arg1	&gamma					415:420	&gamma	415:420	&gamma	415:420	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	2	10	dep	%	297:297	arg1	chains					423:428	chains	423:428	chains	423:428	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	1	11	theme	fish	238:241	arg1	skin					213:216	skin	213:216	skin	213:216	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	1	11	theme	fish	238:241	arg1	by-products					200:210	the by-products	196:210	the by-products (skin) of a cartilaginous fish (Mustelus mustelus)	196:261	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	7	12	theme	green	1143:1147	arg1	films					1159:1163	green bioactive films	1143:1163	green bioactive films	1143:1163	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	12	theme	green	1143:1147	arg1	properties					1083:1092	a potential UV barrier properties	1060:1092	a potential UV barrier properties	1060:1092	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	12	theme	green	1143:1147	arg1	activity					1110:1117	antioxidant activity	1098:1117	antioxidant activity	1098:1117	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	13	theme	Collagen-chitosan-based	1021:1043	arg1	biofilm					1045:1051	Collagen-chitosan-based biofilm	1021:1051	Collagen-chitosan-based biofilm	1021:1051	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	14	theme	nutraceutical	1177:1189	arg1	products					1191:1198	nutraceutical products	1177:1198	nutraceutical products	1177:1198	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	15	used	used	1135:1138	arg2	activity					1110:1117	antioxidant activity	1098:1117	antioxidant activity	1098:1117	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	15	used	used	1135:1138	arg2	films					1159:1163	green bioactive films	1143:1163	green bioactive films	1143:1163	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	15	used	used	1135:1138	arg2	properties					1083:1092	a potential UV barrier properties	1060:1092	a potential UV barrier properties	1060:1092	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	16	theme	potential	1062:1070	arg1	barrier					1075:1081	a potential UV barrier	1060:1081	a potential UV barrier properties	1060:1092	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	6	17	theme	collagen-chitosan	923:939	arg1	interaction					941:951	collagen-chitosan interaction	923:951	collagen-chitosan interaction resulting in a modification of the secondary structure of collagen	923:1018	Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen.					
29914092	4	18	theme	only	600:603	arg1	PSC					605:607	only PSC	600:607	only PSC	600:607	Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film.					
29914092	5	19	theme	higher	717:722	arg1	elongation					724:733	higher elongation	717:733	higher elongation	717:733	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	4	20	theme	collagen	584:591	arg1	yield					593:597	collagen yield	584:597	collagen yield	584:597	Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film.					
29914092	5	21	contain	had	686:688	arg2	elongation					724:733	higher elongation	717:733	higher elongation	717:733	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	5	21	contain	had	686:688	arg1	film					681:684	Such film	676:684	Such film	676:684	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	5	21	contain	had	686:688	arg2	strength					704:711	lower tensile strength	690:711	lower tensile strength	690:711	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	2	22	theme	ASC	268:270	arg1	yields					280:285	The ASC and PSC yields	264:285	yields	280:285	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	2	22	theme	ASC	268:270	arg1	%					297:297	23.07%	292:297	23.07%	292:297	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	2	22	theme	ASC	268:270	arg1	Type					367:370	collagen Type I	358:372	collagen Type I	358:372	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	2	23	theme	dry	310:312	arg1	weight					314:319	35.27% dry weight	303:319	35.27% dry weight	303:319	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	5	24	theme	lower	690:694	arg1	strength					704:711	lower tensile strength	690:711	lower tensile strength	690:711	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	4	25	with	combination	621:631	arg1	chitosan					638:645	chitosan	638:645	chitosan	638:645	Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film.					
29914092	5	26	theme	water	786:790	arg1	solubility					792:801	lower water solubility	780:801	lower water solubility	780:801	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	6	27	theme	composite	888:896	arg1	analysis					871:878	FTIR spectra analysis	858:878	FTIR spectra analysis of film composite	858:896	Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen.					
29914092	2	28	theme	collagen	358:365	arg1	Type					367:370	collagen Type I	358:372	collagen Type I	358:372	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	2	28	theme	collagen	358:365	arg1	yields					280:285	The ASC and PSC yields	264:285	yields	280:285	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	5	29	theme	lower	780:784	arg1	solubility					792:801	lower water solubility	780:801	lower water solubility	780:801	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	1	30	theme	acid	106:109	arg1	ASC					133:135	ASC	133:135	ASC	133:135	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	1	30	theme	acid	106:109	arg1	collagen					123:130	The acid solubilised collagen	102:130	The acid solubilised collagen (ASC)	102:136	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	3	31	dep	Infrared	468:475	arg1	FTIR					478:481	FTIR	478:481	FTIR	478:481	As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure.					
29914092	6	32	theme	film	883:886	arg1	composite					888:896	film composite	883:896	film composite	883:896	Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen.					
29914092	2	33	theme	&alpha	395:400	arg1	presence					383:390	the presence	379:390	the presence of &alpha	379:400	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	7	34	theme	bioactive	1149:1157	arg1	films					1159:1163	green bioactive films	1143:1163	green bioactive films	1143:1163	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	34	theme	bioactive	1149:1157	arg1	properties					1083:1092	a potential UV barrier properties	1060:1092	a potential UV barrier properties	1060:1092	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	34	theme	bioactive	1149:1157	arg1	activity					1110:1117	antioxidant activity	1098:1117	antioxidant activity	1098:1117	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	3	35	theme	PSC	527:529	arg1	structure					544:552	the PSC triple helix structure	523:552	the PSC triple helix structure	523:552	As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure.					
29914092	3	36	theme	Infrared	468:475	arg1	analysis					492:499	Infrared (FTIR) spectra analysis	468:499	Infrared (FTIR) spectra analysis	468:499	As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure.					
29914092	3	37	theme	spectra	484:490	arg1	analysis					492:499	Infrared (FTIR) spectra analysis	468:499	Infrared (FTIR) spectra analysis	468:499	As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure.					
29914092	5	38	theme	tensile	696:702	arg1	strength					704:711	lower tensile strength	690:711	lower tensile strength	690:711	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	0	39	theme	Potential	51:59	arg1	Application					61:71	a Potential Application	49:71	a Potential Application in Bioactive Film Composite	49:99	Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite.					
29914092	7	40	theme	UV	1072:1073	arg1	barrier					1075:1081	a potential UV barrier	1060:1081	a potential UV barrier properties	1060:1092	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	5	41	theme	Such	676:679	arg1	film					681:684	Such film	676:684	Such film	676:684	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	0	42	from	Application	61:71	arg1	Composite					91:99	Bioactive Film Composite	76:99	Bioactive Film Composite	76:99	Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite.					
29914092	1	43	theme	solubilised	111:121	arg1	ASC					133:135	ASC	133:135	ASC	133:135	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	1	43	theme	solubilised	111:121	arg1	collagen					123:130	The acid solubilised collagen	102:130	The acid solubilised collagen (ASC)	102:136	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	5	44	theme	chitosan	761:768	arg1	film					770:773	chitosan film	761:773	chitosan film	761:773	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	1	45	dep	fish	238:241	arg1	mustelus					253:260	Mustelus mustelus	244:260	Mustelus mustelus	244:260	The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (Mustelus mustelus).					
29914092	4	46	theme	composite	660:668	arg1	film					670:673	a composite film	658:673	a composite film	658:673	Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film.					
29914092	4	47	theme	yield	593:597	arg1	type					576:579	the various type	564:579	the various type of collagen yield	564:597	Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film.					
29914092	6	48	theme	structure	998:1006	arg1	modification					968:979	a modification	966:979	a modification of the secondary structure of collagen	966:1018	Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen.					
29914092	6	49	theme	spectra	863:869	arg1	analysis					871:878	FTIR spectra analysis	858:878	FTIR spectra analysis of film composite	858:896	Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen.					
29914092	3	50	theme	triple	531:536	arg1	structure					544:552	the PSC triple helix structure	523:552	the PSC triple helix structure	523:552	As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure.					
29914092	5	51	theme	collagen	834:841	arg1	film					843:846	collagen film	834:846	collagen film	834:846	Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film.					
29914092	2	52	theme	PSC	276:278	arg1	yields					280:285	The ASC and PSC yields	264:285	yields	280:285	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	2	52	theme	PSC	276:278	arg1	%					297:297	23.07%	292:297	23.07%	292:297	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	2	52	theme	PSC	276:278	arg1	Type					367:370	collagen Type I	358:372	collagen Type I	358:372	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	4	53	used	used	613:616	arg2	PSC					605:607	only PSC	600:607	only PSC	600:607	Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film.					
29914092	6	54	theme	FTIR	858:861	arg1	analysis					871:878	FTIR spectra analysis	858:878	FTIR spectra analysis of film composite	858:896	Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen.					
29914092	7	55	theme	antioxidant	1098:1108	arg1	films					1159:1163	green bioactive films	1143:1163	green bioactive films	1143:1163	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	55	theme	antioxidant	1098:1108	arg1	properties					1083:1092	a potential UV barrier properties	1060:1092	a potential UV barrier properties	1060:1092	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	55	theme	antioxidant	1098:1108	arg1	activity					1110:1117	antioxidant activity	1098:1117	antioxidant activity	1098:1117	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	3	56	theme	helix	538:542	arg1	structure					544:552	the PSC triple helix structure	523:552	the PSC triple helix structure	523:552	As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure.					
29914092	2	57	theme	%	308:308	arg1	weight					314:319	35.27% dry weight	303:319	35.27% dry weight	303:319	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	0	58	from	Fish	28:31	arg1	Collagen					0:7	Collagen	0:7	Collagen from Cartilaginous Fish	0:31	Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite.					
29914092	0	59	theme	Cartilaginous	14:26	arg1	Fish					28:31	Cartilaginous Fish	14:31	Cartilaginous Fish	14:31	Collagen from Cartilaginous Fish By-Products for a Potential Application in Bioactive Film Composite.					
29914092	2	60	theme	35.27	303:307	arg1	%					308:308	%	308:308	%	308:308	The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of &alpha;, &beta; and &gamma; chains.					
29914092	7	61	theme	barrier	1075:1081	arg1	films					1159:1163	green bioactive films	1143:1163	green bioactive films	1143:1163	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	61	theme	barrier	1075:1081	arg1	properties					1083:1092	a potential UV barrier properties	1060:1092	a potential UV barrier properties	1060:1092	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
29914092	7	61	theme	barrier	1075:1081	arg1	activity					1110:1117	antioxidant activity	1098:1117	antioxidant activity	1098:1117	Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.					
31830713	0	0	theme	selective	110:118	arg1	adsorption					120:129	highly selective adsorption	103:129	highly selective adsorption with Amberlite XAD-16N	103:152	Investigation on decolorization kinetics and thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N.					
31830713	2	1	theme	intractable	396:406	arg1	contaminations					414:427	intractable color contaminations	396:427	intractable color contaminations derivate from ligoncellulosic degradation	396:469	Selective acidolysis dominates the commercial production of XOS except for intractable color contaminations derivate from ligoncellulosic degradation.					
31830713	6	2	theme	endothermic	1139:1149	arg1	reaction					1151:1158	a spontaneous endothermic reaction	1125:1158	a spontaneous endothermic reaction	1125:1158	The thermodynamics clearly indicated a spontaneous endothermic reaction.					
31830713	1	3	from	booming	186:192	arg1	industries					221:230	food, pharmacy and feed industries	197:230	food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose	197:318	Xylooligosaccharides (XOS) are booming in food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose.					
31830713	0	4	from	Investigation	0:12	arg1	thermodynamics					45:58	thermodynamics	45:58	thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N	45:152	Investigation on decolorization kinetics and thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N.					
31830713	0	4	from	Investigation	0:12	arg1	kinetics					32:39	decolorization kinetics	17:39	decolorization kinetics	17:39	Investigation on decolorization kinetics and thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N.					
31830713	7	5	theme	lignocellulosic	1289:1303	arg1	acidolysis					1305:1314	lignocellulosic acidolysis	1289:1314	lignocellulosic acidolysis	1289:1314	This study provides an important approach for industrial technology development to not only xylooligosaccharides production and lignocellulosic acidolysis, but also Amberlite XAD-16N adsorbent.					
31830713	2	6	theme	Selective	321:329	arg1	acidolysis					331:340	Selective acidolysis	321:340	Selective acidolysis	321:340	Selective acidolysis dominates the commercial production of XOS except for intractable color contaminations derivate from ligoncellulosic degradation.					
31830713	1	7	theme	high-value	279:288	arg1	utilization					290:300	the high-value utilization	275:300	the high-value utilization of lignocellulose	275:318	Xylooligosaccharides (XOS) are booming in food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose.					
31830713	3	8	theme	colored	651:657	arg1	contaminants					659:670	the colored contaminants	647:670	the colored contaminants	647:670	Based on a detailed kinetics and thermodynamics investigation, Amberlite XAD-16N was designated as preferred decolorant because of its high adsorption-selectivity for XOS and the colored contaminants.					
31830713	6	9	theme	spontaneous	1127:1137	arg1	reaction					1151:1158	a spontaneous endothermic reaction	1125:1158	a spontaneous endothermic reaction	1125:1158	The thermodynamics clearly indicated a spontaneous endothermic reaction.					
31830713	5	10	theme	adsorption	983:992	arg1	mechanism					994:1002	The adsorption mechanism	979:1002	The adsorption mechanism	979:1002	The adsorption mechanism was also verified by the adsorption-simulation of three detected typical colorants.					
31830713	3	11	theme	high	607:610	arg1	adsorption-selectivity					612:633	its high adsorption-selectivity	603:633	its high adsorption-selectivity for XOS and the colored contaminants	603:670	Based on a detailed kinetics and thermodynamics investigation, Amberlite XAD-16N was designated as preferred decolorant because of its high adsorption-selectivity for XOS and the colored contaminants.					
31830713	3	12	theme	detailed	483:490	arg1	investigation					520:532	a detailed kinetics and thermodynamics investigation	481:532	a detailed kinetics and thermodynamics investigation	481:532	Based on a detailed kinetics and thermodynamics investigation, Amberlite XAD-16N was designated as preferred decolorant because of its high adsorption-selectivity for XOS and the colored contaminants.					
31830713	1	13	theme	food	197:200	arg1	industries					221:230	food, pharmacy and feed industries	197:230	food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose	197:318	Xylooligosaccharides (XOS) are booming in food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose.					
31830713	0	14	theme	Amberlite	136:144	arg1	XAD-16N					146:152	Amberlite XAD-16N	136:152	Amberlite XAD-16N	136:152	Investigation on decolorization kinetics and thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N.					
31830713	4	15	theme	acidolysis	732:741	arg1	solution					743:750	lignocellulosic acidolysis solution	716:750	lignocellulosic acidolysis solution	716:750	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	5	16	theme	typical	1069:1075	arg1	colorants					1077:1085	three detected typical colorants	1054:1085	three detected typical colorants	1054:1085	The adsorption mechanism was also verified by the adsorption-simulation of three detected typical colorants.					
31830713	2	17	theme	ligoncellulosic	443:457	arg1	degradation					459:469	ligoncellulosic degradation	443:469	ligoncellulosic degradation	443:469	Selective acidolysis dominates the commercial production of XOS except for intractable color contaminations derivate from ligoncellulosic degradation.					
31830713	2	18	theme	commercial	356:365	arg1	production					367:376	the commercial production	352:376	the commercial production of XOS	352:383	Selective acidolysis dominates the commercial production of XOS except for intractable color contaminations derivate from ligoncellulosic degradation.					
31830713	1	19	theme	pharmacy	203:210	arg1	industries					221:230	food, pharmacy and feed industries	197:230	food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose	197:318	Xylooligosaccharides (XOS) are booming in food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose.					
31830713	7	20	theme	xylooligosaccharides	1253:1272	arg1	production					1274:1283	xylooligosaccharides production	1253:1283	xylooligosaccharides production	1253:1283	This study provides an important approach for industrial technology development to not only xylooligosaccharides production and lignocellulosic acidolysis, but also Amberlite XAD-16N adsorbent.					
31830713	4	21	theme	Pseudo-second-order	827:845	arg1	model					847:851	Pseudo-second-order model	827:851	Pseudo-second-order model	827:851	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	2	22	theme	derivate	429:436	arg1	contaminations					414:427	intractable color contaminations	396:427	intractable color contaminations derivate from ligoncellulosic degradation	396:469	Selective acidolysis dominates the commercial production of XOS except for intractable color contaminations derivate from ligoncellulosic degradation.					
31830713	1	23	theme	lignocellulose	305:318	arg1	utilization					290:300	the high-value utilization	275:300	the high-value utilization of lignocellulose	275:318	Xylooligosaccharides (XOS) are booming in food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose.					
31830713	0	24	with	adsorption	120:129	arg1	XAD-16N					146:152	Amberlite XAD-16N	136:152	Amberlite XAD-16N	136:152	Investigation on decolorization kinetics and thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N.					
31830713	4	25	from	solution	743:750	arg1	adsorption					677:686	The adsorption	673:686	The adsorption of the main compositions in lignocellulosic acidolysis solution	673:750	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	1	26	theme	feed	216:219	arg1	industries					221:230	food, pharmacy and feed industries	197:230	food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose	197:318	Xylooligosaccharides (XOS) are booming in food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose.					
31830713	0	27	theme	decolorization	17:30	arg1	kinetics					32:39	decolorization kinetics	17:39	decolorization kinetics	17:39	Investigation on decolorization kinetics and thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N.					
31830713	5	28	theme	colorants	1077:1085	arg1	adsorption-simulation					1029:1049	the adsorption-simulation	1025:1049	the adsorption-simulation of three detected typical colorants	1025:1085	The adsorption mechanism was also verified by the adsorption-simulation of three detected typical colorants.					
31830713	4	29	theme	lignocellulosic	716:730	arg1	solution					743:750	lignocellulosic acidolysis solution	716:750	lignocellulosic acidolysis solution	716:750	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	3	30	theme	preferred	571:579	arg1	decolorant					581:590	preferred decolorant	571:590	preferred decolorant	571:590	Based on a detailed kinetics and thermodynamics investigation, Amberlite XAD-16N was designated as preferred decolorant because of its high adsorption-selectivity for XOS and the colored contaminants.					
31830713	3	30	theme	preferred	571:579	arg1	XAD-16N					545:551	Amberlite XAD-16N	535:551	Amberlite XAD-16N	535:551	Based on a detailed kinetics and thermodynamics investigation, Amberlite XAD-16N was designated as preferred decolorant because of its high adsorption-selectivity for XOS and the colored contaminants.					
31830713	7	31	theme	technology	1218:1227	arg1	development					1229:1239	industrial technology development	1207:1239	industrial technology development	1207:1239	This study provides an important approach for industrial technology development to not only xylooligosaccharides production and lignocellulosic acidolysis, but also Amberlite XAD-16N adsorbent.					
31830713	3	32	theme	kinetics	492:499	arg1	investigation					520:532	a detailed kinetics and thermodynamics investigation	481:532	a detailed kinetics and thermodynamics investigation	481:532	Based on a detailed kinetics and thermodynamics investigation, Amberlite XAD-16N was designated as preferred decolorant because of its high adsorption-selectivity for XOS and the colored contaminants.					
31830713	7	33	theme	adsorbent	1344:1352	arg1	XAD-16N					1336:1342	Amberlite XAD-16N adsorbent	1326:1352	Amberlite XAD-16N adsorbent	1326:1352	This study provides an important approach for industrial technology development to not only xylooligosaccharides production and lignocellulosic acidolysis, but also Amberlite XAD-16N adsorbent.					
31830713	4	34	from	adsorption	677:686	arg1	solution					743:750	lignocellulosic acidolysis solution	716:750	lignocellulosic acidolysis solution	716:750	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	0	35	theme	lignocellulosic	63:77	arg1	xylooligosaccharides					79:98	lignocellulosic xylooligosaccharides	63:98	lignocellulosic xylooligosaccharides	63:98	Investigation on decolorization kinetics and thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N.					
31830713	4	36	theme	Langmuir	774:781	arg1	models					783:788	Langmuir models	774:788	Langmuir models	774:788	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	4	37	from	compositions	700:711	arg1	solution					743:750	lignocellulosic acidolysis solution	716:750	lignocellulosic acidolysis solution	716:750	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	4	38	theme	cross-linking	944:956	arg1	groups					958:963	the resin cross-linking groups	934:963	the resin cross-linking groups	934:963	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	7	39	theme	Amberlite	1326:1334	arg1	XAD-16N					1336:1342	Amberlite XAD-16N adsorbent	1326:1352	Amberlite XAD-16N adsorbent	1326:1352	This study provides an important approach for industrial technology development to not only xylooligosaccharides production and lignocellulosic acidolysis, but also Amberlite XAD-16N adsorbent.					
31830713	7	40	theme	industrial	1207:1216	arg1	development					1229:1239	industrial technology development	1207:1239	industrial technology development	1207:1239	This study provides an important approach for industrial technology development to not only xylooligosaccharides production and lignocellulosic acidolysis, but also Amberlite XAD-16N adsorbent.					
31830713	4	41	theme	resin	938:942	arg1	groups					958:963	the resin cross-linking groups	934:963	the resin cross-linking groups	934:963	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	2	42	from	degradation	459:469	arg1	derivate					429:436	derivate	429:436	derivate	429:436	Selective acidolysis dominates the commercial production of XOS except for intractable color contaminations derivate from ligoncellulosic degradation.					
31830713	2	43	theme	XOS	381:383	arg1	production					367:376	the commercial production	352:376	the commercial production of XOS	352:383	Selective acidolysis dominates the commercial production of XOS except for intractable color contaminations derivate from ligoncellulosic degradation.					
31830713	7	44	theme	important	1184:1192	arg1	approach					1194:1201	an important approach	1181:1201	an important approach for industrial technology development to not only xylooligosaccharides production and lignocellulosic acidolysis	1181:1314	This study provides an important approach for industrial technology development to not only xylooligosaccharides production and lignocellulosic acidolysis, but also Amberlite XAD-16N adsorbent.					
31830713	1	45	from	industries	221:230	arg1	booming					186:192	booming	186:192	booming	186:192	Xylooligosaccharides (XOS) are booming in food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose.					
31830713	4	46	theme	main	695:698	arg1	compositions					700:711	the main compositions	691:711	the main compositions in lignocellulosic acidolysis solution	691:750	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	5	47	theme	detected	1060:1067	arg1	colorants					1077:1085	three detected typical colorants	1054:1085	three detected typical colorants	1054:1085	The adsorption mechanism was also verified by the adsorption-simulation of three detected typical colorants.					
31830713	3	48	theme	Amberlite	535:543	arg1	decolorant					581:590	preferred decolorant	571:590	preferred decolorant	571:590	Based on a detailed kinetics and thermodynamics investigation, Amberlite XAD-16N was designated as preferred decolorant because of its high adsorption-selectivity for XOS and the colored contaminants.					
31830713	3	48	theme	Amberlite	535:543	arg1	XAD-16N					545:551	Amberlite XAD-16N	535:551	Amberlite XAD-16N	535:551	Based on a detailed kinetics and thermodynamics investigation, Amberlite XAD-16N was designated as preferred decolorant because of its high adsorption-selectivity for XOS and the colored contaminants.					
31830713	3	49	theme	thermodynamics	505:518	arg1	investigation					520:532	a detailed kinetics and thermodynamics investigation	481:532	a detailed kinetics and thermodynamics investigation	481:532	Based on a detailed kinetics and thermodynamics investigation, Amberlite XAD-16N was designated as preferred decolorant because of its high adsorption-selectivity for XOS and the colored contaminants.					
31830713	1	50	theme	great	257:261	arg1	interest					263:270	great interest	257:270	great interest	257:270	Xylooligosaccharides (XOS) are booming in food, pharmacy and feed industries that they have attracted great interest in the high-value utilization of lignocellulose.					
31830713	0	51	theme	xylooligosaccharides	79:98	arg1	thermodynamics					45:58	thermodynamics	45:58	thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N	45:152	Investigation on decolorization kinetics and thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N.					
31830713	0	51	theme	xylooligosaccharides	79:98	arg1	kinetics					32:39	decolorization kinetics	17:39	decolorization kinetics	17:39	Investigation on decolorization kinetics and thermodynamics of lignocellulosic xylooligosaccharides by highly selective adsorption with Amberlite XAD-16N.					
31830713	4	52	theme	electron	900:907	arg1	sharing/transfer					909:924	electron sharing/transfer	900:924	electron sharing/transfer between the resin cross-linking groups and colorant	900:976	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
31830713	2	53	theme	color	408:412	arg1	contaminations					414:427	intractable color contaminations	396:427	intractable color contaminations derivate from ligoncellulosic degradation	396:469	Selective acidolysis dominates the commercial production of XOS except for intractable color contaminations derivate from ligoncellulosic degradation.					
31830713	4	54	theme	compositions	700:711	arg1	adsorption					677:686	The adsorption	673:686	The adsorption of the main compositions in lignocellulosic acidolysis solution	673:750	The adsorption of the main compositions in lignocellulosic acidolysis solution was well described by Langmuir models, the kinetics were clearly fitted by Pseudo-second-order model, showing that the adsorption was controlled by electron sharing/transfer between the resin cross-linking groups and colorant.					
29843481	5	0	theme	combined	1062:1069	arg1	pretreatment					1071:1082	the combined pretreatment	1058:1082	the combined pretreatment	1058:1082	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	6	1	theme	crystal	1214:1220	arg1	indices					1222:1228	the cellulose crystal indices	1200:1228	the cellulose crystal indices	1200:1228	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	3	2	theme	ozone	464:468	arg1	treatment					470:478	ozone treatment	464:478	ozone treatment	464:478	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	1	3	theme	corn	253:256	arg1	stover					258:263	corn stover	253:263	corn stover	253:263	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	3	4	theme	hydrolysis	609:618	arg1	efficiency					566:575	the maximum efficiency	554:575	the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis	554:618	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	3	4	theme	hydrolysis	609:618	arg1	%					583:583	91.73%	578:583	91.73%	578:583	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	6	5	theme	cellulose	1204:1212	arg1	indices					1222:1228	the cellulose crystal indices	1200:1228	the cellulose crystal indices	1200:1228	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	3	6	dep	found	516:520	arg1	procedure					540:548	the optimal procedure	528:548	the optimal procedure	528:548	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	6	7	theme	bonds	1369:1373	arg1	disruption					1349:1358	disruption	1349:1358	disruption of ester bonds in hemicellulose	1349:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	7	theme	bonds	1369:1373	arg1	cellulose					1335:1343	cellulose	1335:1343	cellulose	1335:1343	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	1	8	theme	stover	258:263	arg1	characteristics					234:248	structural characteristics	223:248	structural characteristics	223:248	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	1	8	theme	stover	258:263	arg1	hydrolysis					194:203	the enzymatic hydrolysis	180:203	the enzymatic hydrolysis	180:203	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	1	8	theme	stover	258:263	arg1	compositions					206:217	compositions	206:217	compositions	206:217	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	0	9	theme	Combined	87:94	arg1	Pretreatment					96:107	Ozone Combined Pretreatment	81:107	Ozone Combined Pretreatment	81:107	Enhanced Enzymatic Hydrolysis and Structural Features of Corn Stover by NaOH and Ozone Combined Pretreatment.					
29843481	4	10	theme	samples	714:720	arg1	structure					697:705	the surface structure	685:705	the surface structure of the samples	685:720	Furthermore, microscopic observation of changes in the surface structure of the samples showed that holes were formed and lignin and hemicellulose were partially dissolved and removed.					
29843481	6	11	theme	bonds	1405:1409	arg1	linkage					1411:1417	bonds linkage	1405:1417	bonds linkage in lignin-carbohydrate complexes	1405:1450	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	3	12	with	pretreatment	403:414	arg1	NaOH					430:433	2% (w/w) NaOH	421:433	2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min	421:489	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	3	12	with	pretreatment	403:414	arg1	pH					507:508	an initial pH 9	496:510	an initial pH 9	496:510	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	6	13	theme	ester	1363:1367	arg1	bonds					1369:1373	ester bonds	1363:1373	ester bonds in hemicellulose	1363:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	0	14	theme	Ozone	81:85	arg1	Pretreatment					96:107	Ozone Combined Pretreatment	81:107	Ozone Combined Pretreatment	81:107	Enhanced Enzymatic Hydrolysis and Structural Features of Corn Stover by NaOH and Ozone Combined Pretreatment.					
29843481	5	15	theme	chemical	1024:1031	arg1	changes					1044:1050	the chemical structural changes	1020:1050	the chemical structural changes	1020:1050	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	3	16	theme	initial	499:505	arg1	pH					507:508	an initial pH 9	496:510	an initial pH 9	496:510	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	4	17	from	structure	697:705	arg1	observation					659:669	microscopic observation	647:669	microscopic observation of changes in the surface structure of the samples	647:720	Furthermore, microscopic observation of changes in the surface structure of the samples showed that holes were formed and lignin and hemicellulose were partially dissolved and removed.					
29843481	2	18	theme	unpretreated	289:300	arg1	stover					322:327	the unpretreated and pretreated corn stover	285:327	the unpretreated and pretreated corn stover	285:327	Comparison between the unpretreated and pretreated corn stover was also made to illustrate the mechanism of the combined pretreatment.					
29843481	6	19	theme	methoxy	1464:1470	arg1	removal					1453:1459	removal	1453:1459	removal of methoxy in lignin and hemicellulose	1453:1498	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	5	20	theme	structural	1033:1042	arg1	changes					1044:1050	the chemical structural changes	1020:1050	the chemical structural changes	1020:1050	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	6	21	from	removal	1453:1459	arg1	hemicellulose					1486:1498	hemicellulose	1486:1498	hemicellulose	1486:1498	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	21	from	removal	1453:1459	arg1	lignin					1475:1480	lignin	1475:1480	lignin	1475:1480	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	5	22	theme	Cross-Polarization	895:912	arg1	Angle					920:924	Cross-Polarization Magic Angle	895:924	Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR)	895:987	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	6	23	theme	I	1136:1136	arg1	structure					1138:1146	the cellulose I structure	1122:1146	the cellulose I structure	1122:1146	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	5	24	theme	Infrared	862:869	arg1	FTIR					885:888	FTIR	885:888	FTIR	885:888	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	24	theme	Infrared	862:869	arg1	Spectroscopy					871:882	Infrared Spectroscopy	862:882	Infrared Spectroscopy (FTIR)	862:889	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	7	25	theme	subsequent	1594:1603	arg1	hydrolysis					1615:1624	subsequent enzymatic hydrolysis	1594:1624	subsequent enzymatic hydrolysis of cellulose	1594:1637	As a result, all these changes effectively reduced recalcitrance of corn stover and promoted subsequent enzymatic hydrolysis of cellulose.					
29843481	7	26	theme	cellulose	1629:1637	arg1	hydrolysis					1615:1624	subsequent enzymatic hydrolysis	1594:1624	subsequent enzymatic hydrolysis of cellulose	1594:1637	As a result, all these changes effectively reduced recalcitrance of corn stover and promoted subsequent enzymatic hydrolysis of cellulose.					
29843481	0	27	theme	Enzymatic	9:17	arg1	Hydrolysis					19:28	Enzymatic Hydrolysis	9:28	Enzymatic Hydrolysis	9:28	Enhanced Enzymatic Hydrolysis and Structural Features of Corn Stover by NaOH and Ozone Combined Pretreatment.					
29843481	4	28	theme	changes	674:680	arg1	observation					659:669	microscopic observation	647:669	microscopic observation of changes in the surface structure of the samples	647:720	Furthermore, microscopic observation of changes in the surface structure of the samples showed that holes were formed and lignin and hemicellulose were partially dissolved and removed.					
29843481	7	29	theme	enzymatic	1605:1613	arg1	hydrolysis					1615:1624	subsequent enzymatic hydrolysis	1594:1624	subsequent enzymatic hydrolysis of cellulose	1594:1637	As a result, all these changes effectively reduced recalcitrance of corn stover and promoted subsequent enzymatic hydrolysis of cellulose.					
29843481	2	30	theme	pretreatment	387:398	arg1	mechanism					361:369	the mechanism	357:369	the mechanism of the combined pretreatment	357:398	Comparison between the unpretreated and pretreated corn stover was also made to illustrate the mechanism of the combined pretreatment.					
29843481	6	31	from	disruption	1303:1312	arg1	disruption					1349:1358	disruption	1349:1358	disruption of ester bonds in hemicellulose	1349:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	31	from	disruption	1303:1312	arg1	cellulose					1335:1343	cellulose	1335:1343	cellulose	1335:1343	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	32	attach	removal	1453:1459	arg2	methoxy					1464:1470	methoxy	1464:1470	methoxy	1464:1470	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	32	attach	removal	1453:1459	arg3	lignin					1475:1480	lignin	1475:1480	lignin	1475:1480	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	32	attach	removal	1453:1459	arg3	hemicellulose					1486:1498	hemicellulose	1486:1498	hemicellulose	1486:1498	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	4	33	from	changes	674:680	arg1	structure					697:705	the surface structure	685:705	the surface structure of the samples	685:720	Furthermore, microscopic observation of changes in the surface structure of the samples showed that holes were formed and lignin and hemicellulose were partially dissolved and removed.					
29843481	6	34	from	linkage	1411:1417	arg1	complexes					1442:1450	lignin-carbohydrate complexes	1422:1450	lignin-carbohydrate complexes	1422:1450	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	2	35	theme	combined	378:385	arg1	pretreatment					387:398	the combined pretreatment	374:398	the combined pretreatment	374:398	Comparison between the unpretreated and pretreated corn stover was also made to illustrate the mechanism of the combined pretreatment.					
29843481	6	36	theme	cellulose	1126:1134	arg1	structure					1138:1146	the cellulose I structure	1122:1146	the cellulose I structure	1122:1146	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	37	from	complexes	1442:1450	arg1	cleavage					1393:1400	cleavage	1393:1400	cleavage of bonds linkage in lignin-carbohydrate complexes	1393:1450	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	2	38	theme	corn	317:320	arg1	stover					322:327	the unpretreated and pretreated corn stover	285:327	the unpretreated and pretreated corn stover	285:327	Comparison between the unpretreated and pretreated corn stover was also made to illustrate the mechanism of the combined pretreatment.					
29843481	6	39	theme	lignin-carbohydrate	1422:1440	arg1	complexes					1442:1450	lignin-carbohydrate complexes	1422:1450	lignin-carbohydrate complexes	1422:1450	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	1	40	theme	enzymatic	184:192	arg1	characteristics					234:248	structural characteristics	223:248	structural characteristics	223:248	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	1	40	theme	enzymatic	184:192	arg1	hydrolysis					194:203	the enzymatic hydrolysis	180:203	the enzymatic hydrolysis	180:203	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	1	40	theme	enzymatic	184:192	arg1	compositions					206:217	compositions	206:217	compositions	206:217	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	6	41	theme	wider	1251:1255	arg1	space					1257:1261	a wider space	1249:1261	a wider space between the crystal layer	1249:1287	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	42	theme	bonds	1326:1330	arg1	disruption					1303:1312	disruption	1303:1312	disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose	1303:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	2	43	theme	pretreated	306:315	arg1	stover					322:327	the unpretreated and pretreated corn stover	285:327	the unpretreated and pretreated corn stover	285:327	Comparison between the unpretreated and pretreated corn stover was also made to illustrate the mechanism of the combined pretreatment.					
29843481	7	44	theme	corn	1569:1572	arg1	stover					1574:1579	corn stover	1569:1579	corn stover	1569:1579	As a result, all these changes effectively reduced recalcitrance of corn stover and promoted subsequent enzymatic hydrolysis of cellulose.					
29843481	6	45	dep	were	1097:1100	arg1	cleavage					1393:1400	cleavage	1393:1400	cleavage of bonds linkage in lignin-carbohydrate complexes	1393:1450	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	45	dep	were	1097:1100	arg1	removal					1453:1459	removal	1453:1459	removal of methoxy in lignin and hemicellulose	1453:1498	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	45	dep	were	1097:1100	arg1	changed					1240:1246	changed	1240:1246	were also changed	1230:1246	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	45	dep	were	1097:1100	arg1	disruption					1303:1312	disruption	1303:1312	disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose	1303:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	46	from	cellulose	1335:1343	arg1	hemicellulose					1378:1390	hemicellulose	1378:1390	hemicellulose	1378:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	46	from	cellulose	1335:1343	arg1	disruption					1303:1312	disruption	1303:1312	disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose	1303:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	5	47	theme	Magnetic	953:960	arg1	Resonance					962:970	Carbon-13 Nuclear Magnetic Resonance	935:970	Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR)	935:987	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	47	theme	Magnetic	953:960	arg1	13C-NMR					980:986	CP/MAS 13C-NMR	973:986	CP/MAS 13C-NMR	973:986	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	6	48	theme	linkage	1411:1417	arg1	cleavage					1393:1400	cleavage	1393:1400	cleavage of bonds linkage in lignin-carbohydrate complexes	1393:1450	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	0	49	theme	Structural	34:43	arg1	Features					45:52	Structural Features	34:52	Structural Features of Corn Stover	34:67	Enhanced Enzymatic Hydrolysis and Structural Features of Corn Stover by NaOH and Ozone Combined Pretreatment.					
29843481	3	50	theme	%	422:422	arg1	NaOH					430:433	2% (w/w) NaOH	421:433	2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min	421:489	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	4	51	theme	surface	689:695	arg1	structure					697:705	the surface structure	685:705	the surface structure of the samples	685:720	Furthermore, microscopic observation of changes in the surface structure of the samples showed that holes were formed and lignin and hemicellulose were partially dissolved and removed.					
29843481	3	52	theme	optimal	532:538	arg1	procedure					540:548	the optimal procedure	528:548	the optimal procedure	528:548	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	0	53	theme	Corn	57:60	arg1	Stover					62:67	Corn Stover	57:67	Corn Stover	57:67	Enhanced Enzymatic Hydrolysis and Structural Features of Corn Stover by NaOH and Ozone Combined Pretreatment.					
29843481	5	54	dep	Fourier	844:850	arg1	Transform					852:860	Transform	852:860	Transform Infrared Spectroscopy (FTIR)	852:889	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	6	55	from	disruption	1349:1358	arg1	hemicellulose					1378:1390	hemicellulose	1378:1390	hemicellulose	1378:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	55	from	disruption	1349:1358	arg1	disruption					1303:1312	disruption	1303:1312	disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose	1303:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	56	from	bonds	1369:1373	arg1	hemicellulose					1378:1390	hemicellulose	1378:1390	hemicellulose	1378:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	57	theme	hydrogen	1317:1324	arg1	bonds					1326:1330	hydrogen bonds	1317:1330	hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose	1317:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	3	58	theme	cellulose	589:597	arg1	hydrolysis					609:618	cellulose enzymatic hydrolysis	589:618	cellulose enzymatic hydrolysis	589:618	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	5	59	theme	CP/MAS	973:978	arg1	Resonance					962:970	Carbon-13 Nuclear Magnetic Resonance	935:970	Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR)	935:987	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	59	theme	CP/MAS	973:978	arg1	13C-NMR					980:986	CP/MAS 13C-NMR	973:986	CP/MAS 13C-NMR	973:986	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	3	60	from	°C	441:442	arg1	NaOH					430:433	2% (w/w) NaOH	421:433	2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min	421:489	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	1	61	theme	two-step	112:119	arg1	pretreatment					121:132	A two-step pretreatment	110:132	A two-step pretreatment using NaOH and ozone	110:153	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	4	62	from	observation	659:669	arg1	structure					697:705	the surface structure	685:705	the surface structure of the samples	685:720	Furthermore, microscopic observation of changes in the surface structure of the samples showed that holes were formed and lignin and hemicellulose were partially dissolved and removed.					
29843481	5	63	used	used	999:1002	arg2	Angle					920:924	Cross-Polarization Magic Angle	895:924	Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR)	895:987	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	63	used	used	999:1002	arg2	Fourier					844:850	Fourier	844:850	Fourier Transform Infrared Spectroscopy (FTIR)	844:889	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	63	used	used	999:1002	arg2	XRD					838:840	XRD	838:840	XRD	838:840	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	63	used	used	999:1002	arg2	Diffraction					825:835	X-ray Diffraction	819:835	X-ray Diffraction (XRD)	819:841	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	3	64	theme	enzymatic	599:607	arg1	hydrolysis					609:618	cellulose enzymatic hydrolysis	589:618	cellulose enzymatic hydrolysis	589:618	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	6	65	theme	structure	1138:1146	arg1	part					1114:1117	part	1114:1117	part of the cellulose I structure	1114:1146	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	66	from	hemicellulose	1378:1390	arg1	disruption					1349:1358	disruption	1349:1358	disruption of ester bonds in hemicellulose	1349:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	66	from	hemicellulose	1378:1390	arg1	cellulose					1335:1343	cellulose	1335:1343	cellulose	1335:1343	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	5	67	theme	Magic	914:918	arg1	Angle					920:924	Cross-Polarization Magic Angle	895:924	Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR)	895:987	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	3	68	theme	maximum	558:564	arg1	efficiency					566:575	the maximum efficiency	554:575	the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis	554:618	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	3	68	theme	maximum	558:564	arg1	%					583:583	91.73%	578:583	91.73%	578:583	A pretreatment with 2% (w/w) NaOH at 80 °C for 2 h followed by ozone treatment for 25 min with an initial pH 9 was found to be the optimal procedure and the maximum efficiency (91.73%) of cellulose enzymatic hydrolysis was achieved.					
29843481	7	69	theme	stover	1574:1579	arg1	recalcitrance					1552:1564	recalcitrance	1552:1564	recalcitrance of corn stover	1552:1579	As a result, all these changes effectively reduced recalcitrance of corn stover and promoted subsequent enzymatic hydrolysis of cellulose.					
29843481	7	69	theme	stover	1574:1579	arg1	result					1506:1511	a result	1504:1511	a result	1504:1511	As a result, all these changes effectively reduced recalcitrance of corn stover and promoted subsequent enzymatic hydrolysis of cellulose.					
29843481	4	70	theme	microscopic	647:657	arg1	observation					659:669	microscopic observation	647:669	microscopic observation of changes in the surface structure of the samples	647:720	Furthermore, microscopic observation of changes in the surface structure of the samples showed that holes were formed and lignin and hemicellulose were partially dissolved and removed.					
29843481	6	71	from	cleavage	1393:1400	arg1	complexes					1442:1450	lignin-carbohydrate complexes	1422:1450	lignin-carbohydrate complexes	1422:1450	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	1	72	theme	structural	223:232	arg1	characteristics					234:248	structural characteristics	223:248	structural characteristics	223:248	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	1	72	theme	structural	223:232	arg1	hydrolysis					194:203	the enzymatic hydrolysis	180:203	the enzymatic hydrolysis	180:203	A two-step pretreatment using NaOH and ozone was performed to improve the enzymatic hydrolysis, compositions and structural characteristics of corn stover.					
29843481	6	73	from	bonds	1326:1330	arg1	disruption					1349:1358	disruption	1349:1358	disruption of ester bonds in hemicellulose	1349:1390	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	6	73	from	bonds	1326:1330	arg1	cellulose					1335:1343	cellulose	1335:1343	cellulose	1335:1343	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
29843481	5	74	theme	Carbon-13	935:943	arg1	Resonance					962:970	Carbon-13 Nuclear Magnetic Resonance	935:970	Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR)	935:987	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	74	theme	Carbon-13	935:943	arg1	13C-NMR					980:986	CP/MAS 13C-NMR	973:986	CP/MAS 13C-NMR	973:986	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	75	theme	X-ray	819:823	arg1	XRD					838:840	XRD	838:840	XRD	838:840	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	75	theme	X-ray	819:823	arg1	Diffraction					825:835	X-ray Diffraction	819:835	X-ray Diffraction (XRD)	819:841	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	0	76	theme	Stover	62:67	arg1	Hydrolysis					19:28	Enzymatic Hydrolysis	9:28	Enzymatic Hydrolysis	9:28	Enhanced Enzymatic Hydrolysis and Structural Features of Corn Stover by NaOH and Ozone Combined Pretreatment.					
29843481	0	76	theme	Stover	62:67	arg1	Features					45:52	Structural Features	34:52	Structural Features of Corn Stover	34:67	Enhanced Enzymatic Hydrolysis and Structural Features of Corn Stover by NaOH and Ozone Combined Pretreatment.					
29843481	5	77	theme	Nuclear	945:951	arg1	Resonance					962:970	Carbon-13 Nuclear Magnetic Resonance	935:970	Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR)	935:987	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	5	77	theme	Nuclear	945:951	arg1	13C-NMR					980:986	CP/MAS 13C-NMR	973:986	CP/MAS 13C-NMR	973:986	X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Cross-Polarization Magic Angle Spinning Carbon-13 Nuclear Magnetic Resonance (CP/MAS 13C-NMR) were also used to characterize the chemical structural changes after the combined pretreatment.					
29843481	6	78	theme	crystal	1275:1281	arg1	layer					1283:1287	the crystal layer	1271:1287	the crystal layer	1271:1287	The results were as follows: part of the cellulose I structure was destroyed and then reformed into cellulose III, the cellulose crystal indices were also changed; a wider space between the crystal layer was observed; disruption of hydrogen bonds in cellulose and disruption of ester bonds in hemicellulose; cleavage of bonds linkage in lignin-carbohydrate complexes; removal of methoxy in lignin and hemicellulose.					
30797108	2	0	theme	transfer	601:608	arg1	kinetics					610:617	higher electron transfer kinetics	585:617	higher electron transfer kinetics	585:617	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	7	1	theme	considerable	1708:1719	arg1	advantages					1721:1730	considerable advantages	1708:1730	considerable advantages in terms of reproducibility, selectivity	1708:1771	This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface.					
30797108	2	2	theme	higher	585:590	arg1	kinetics					610:617	higher electron transfer kinetics	585:617	higher electron transfer kinetics	585:617	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	3	3	theme	III	980:982	arg1	detection					964:972	the detection	960:972	the detection of As(III)	960:983	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	5	4	theme	optical	1372:1378	arg1	aptasensor					1388:1397	an optical As(III) aptasensor	1369:1397	an optical As(III) aptasensor using the UV-Vis spectroscopic method	1369:1435	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	6	5	theme	fabricated	1475:1484	arg1	aptasensor					1486:1495	the fabricated aptasensor	1471:1495	the fabricated aptasensor	1471:1495	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	1	6	theme	conductive	234:243	arg1	advantages					171:180	the advantages	167:180	the advantages of chitosan-Nafion (Chit-Naf) composite	167:220	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	6	theme	conductive	234:243	arg1	platform					253:260	a highly conductive surface platform	225:260	a highly conductive surface platform	225:260	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	2	7	theme	bare	632:635	arg1	electrodes					660:669	the bare GC, GC/Naf and GC/Chit electrodes	628:669	electrodes	660:669	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	7	8	theme	sensing	1820:1826	arg1	interface					1828:1836	the sensing interface	1816:1836	the sensing interface	1816:1836	This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface.					
30797108	6	9	theme	discrimination	1442:1455	arg1	capability					1457:1466	The discrimination capability	1438:1466	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples	1438:1599	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	7	10	theme	sensitive	1672:1680	arg1	detection					1682:1690	sensitive detection	1672:1690	sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity	1672:1771	This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface.					
30797108	2	11	theme	GC	534:535	arg1	electrode					538:546	the glassy carbon electrode (GC) electrode	505:546	the glassy carbon electrode (GC) electrode modified with Chit-Naf composite	505:579	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	2	12	theme	assays	760:765	arg1	construction					733:744	construction	733:744	construction of biosensing assays	733:765	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	2	13	from	platform	721:728	arg1	construction					733:744	construction	733:744	construction of biosensing assays	733:765	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	3	14	theme	carbon	835:840	arg1	albumin					864:870	carbon nanotube-bovine serum albumin	835:870	carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system	835:894	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	3	14	theme	carbon	835:840	arg1	CNT-BSA					873:879	CNT-BSA	873:879	CNT-BSA	873:879	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	1	15	theme	chitosan-Nafion	185:199	arg1	composite					212:220	chitosan-Nafion (Chit-Naf) composite	185:220	chitosan-Nafion (Chit-Naf) composite	185:220	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	6	16	theme	water	1587:1591	arg1	samples					1593:1599	waste water samples	1581:1599	waste water samples	1581:1599	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	2	17	theme	great	689:693	arg1	potential					695:703	a great potential	687:703	a great potential	687:703	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	2	17	theme	great	689:693	arg1	platform					721:728	an efficient platform	708:728	an efficient platform in construction of biosensing assays	708:765	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	7	18	theme	new	1657:1659	arg1	method					1661:1666	a new method	1655:1666	a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity	1655:1771	This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface.					
30797108	1	19	theme	III	408:410	arg1	detection					392:400	highly sensitive detection	375:400	highly sensitive detection of As(III)	375:411	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	20	theme	present	125:131	arg1	work					133:136	the present work	121:136	the present work	121:136	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	6	21	theme	complex	1563:1569	arg1	matrix					1571:1576	a complex matrix	1561:1576	a complex matrix of waste water samples	1561:1599	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	4	22	theme	limits	1090:1095	arg1	limits					1090:1095	the lowest limits	1079:1095	the lowest limits of detection for As(III)	1079:1120	This protocol provided one of the lowest limits of detection for As(III) on aptamer-based electrodes recently described in the literature.					
30797108	4	22	theme	limits	1090:1095	arg1	one					1072:1074	one	1072:1074	one	1072:1074	This protocol provided one of the lowest limits of detection for As(III) on aptamer-based electrodes recently described in the literature.					
30797108	5	23	theme	optical	1216:1222	arg1	properties					1235:1244	the optical absorptive properties	1212:1244	the optical absorptive properties of CNTs	1212:1252	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	0	24	theme	impedimetric	68:79	arg1	fabrication					36:46	fabrication	36:46	fabrication of highly sensitive impedimetric and colorimetric	36:96	Novel chitosan-Nafion composite for fabrication of highly sensitive impedimetric and colorimetric As(III) aptasensor.					
30797108	4	25	theme	detection	1100:1108	arg1	limits					1090:1095	the lowest limits	1079:1095	the lowest limits of detection for As(III)	1079:1120	This protocol provided one of the lowest limits of detection for As(III) on aptamer-based electrodes recently described in the literature.					
30797108	3	26	theme	amplification	803:815	arg1	strategy					817:824	a signal amplification strategy	794:824	a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively	794:1046	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	0	27	theme	colorimetric	85:96	arg1	fabrication					36:46	fabrication	36:46	fabrication of highly sensitive impedimetric and colorimetric	36:96	Novel chitosan-Nafion composite for fabrication of highly sensitive impedimetric and colorimetric As(III) aptasensor.					
30797108	3	28	theme	serum	858:862	arg1	albumin					864:870	carbon nanotube-bovine serum albumin	835:870	carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system	835:894	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	3	28	theme	serum	858:862	arg1	CNT-BSA					873:879	CNT-BSA	873:879	CNT-BSA	873:879	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	2	29	theme	electrode	523:531	arg1	electrode					538:546	the glassy carbon electrode (GC) electrode	505:546	the glassy carbon electrode (GC) electrode modified with Chit-Naf composite	505:579	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	5	30	theme	UV-Vis	1409:1414	arg1	method					1430:1435	the UV-Vis spectroscopic method	1405:1435	the UV-Vis spectroscopic method	1405:1435	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	2	31	theme	GC/Naf	641:646	arg1	electrodes					660:669	the bare GC, GC/Naf and GC/Chit electrodes	628:669	electrodes	660:669	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	1	32	theme	lable-free	327:336	arg1	sensor					364:369	a lable-free impedimetricaptamer-based sensor	325:369	a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III)	325:411	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	6	33	theme	ions	1552:1555	arg1	presence					1528:1535	the presence	1524:1535	the presence of other metal ions and a complex matrix of waste water samples	1524:1599	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	2	34	theme	glassy	509:514	arg1	electrode					538:546	the glassy carbon electrode (GC) electrode	505:546	the glassy carbon electrode (GC) electrode modified with Chit-Naf composite	505:579	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	2	35	theme	GC	637:638	arg1	electrodes					660:669	the bare GC, GC/Naf and GC/Chit electrodes	628:669	electrodes	660:669	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	5	36	theme	biorecognition	1259:1272	arg1	interactions					1274:1285	biorecognition interactions	1259:1285	biorecognition interactions	1259:1285	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	6	37	theme	other	1540:1544	arg1	ions					1552:1555	other metal ions	1540:1555	other metal ions	1540:1555	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	2	38	theme	impedance	434:442	arg1	EIS					458:460	EIS	458:460	EIS	458:460	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	2	38	theme	impedance	434:442	arg1	spectroscopy					444:455	electrochemical impedance spectroscopy	418:455	The electrochemical impedance spectroscopy (EIS) investigations	414:476	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	0	39	theme	Novel	0:4	arg1	chitosan-Nafion					6:20	Novel chitosan-Nafion	0:20	Novel chitosan-Nafion	0:20	Novel chitosan-Nafion composite for fabrication of highly sensitive impedimetric and colorimetric As(III) aptasensor.					
30797108	1	40	theme	surface	245:251	arg1	advantages					171:180	the advantages	167:180	the advantages of chitosan-Nafion (Chit-Naf) composite	167:220	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	40	theme	surface	245:251	arg1	platform					253:260	a highly conductive surface platform	225:260	a highly conductive surface platform	225:260	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	3	41	theme	74 pM	1028:1032	arg1	a					1023:1023	a	1023:1023	a	1023:1023	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	3	41	theme	74 pM	1028:1032	arg1	100.82 Ω nM-1					1005:1017	100.82 Ω nM-1	1005:1017	100.82 Ω nM-1	1005:1017	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	3	42	theme	sensitivity	906:916	arg1	limit					932:936	sensitivity and detection limit	906:936	limit	932:936	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	2	43	theme	GC/Chit	652:658	arg1	electrodes					660:669	the bare GC, GC/Naf and GC/Chit electrodes	628:669	electrodes	660:669	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	5	44	theme	properties	1235:1244	arg1	change					1202:1207	the change	1198:1207	the change of the optical absorptive properties of CNTs upon biorecognition interactions	1198:1285	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	2	45	theme	efficient	711:719	arg1	potential					695:703	a great potential	687:703	a great potential	687:703	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	2	45	theme	efficient	711:719	arg1	platform					721:728	an efficient platform	708:728	an efficient platform in construction of biosensing assays	708:765	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	5	46	theme	CNTs	1249:1252	arg1	properties					1235:1244	the optical absorptive properties	1212:1244	the optical absorptive properties of CNTs	1212:1252	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	1	47	theme	novel	268:272	arg1	advantages					171:180	the advantages	167:180	the advantages of chitosan-Nafion (Chit-Naf) composite	167:220	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	47	theme	novel	268:272	arg1	strategy					305:312	a novel CNT-based signal amplification strategy	266:312	a novel CNT-based signal amplification strategy	266:312	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	7	48	theme	reproducibility	1744:1758	arg1	terms					1735:1739	terms	1735:1739	terms of reproducibility, selectivity	1735:1771	This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface.					
30797108	5	49	theme	biorecognition	1316:1329	arg1	process					1331:1337	the biorecognition process	1312:1337	the biorecognition process	1312:1337	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	1	50	theme	signal	284:289	arg1	advantages					171:180	the advantages	167:180	the advantages of chitosan-Nafion (Chit-Naf) composite	167:220	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	50	theme	signal	284:289	arg1	strategy					305:312	a novel CNT-based signal amplification strategy	266:312	a novel CNT-based signal amplification strategy	266:312	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	3	51	theme	detection	922:930	arg1	limit					932:936	sensitivity and detection limit	906:936	limit	932:936	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	3	52	theme	aptasensor	945:954	arg1	limit					932:936	sensitivity and detection limit	906:936	limit	932:936	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	2	53	theme	electron	592:599	arg1	kinetics					610:617	higher electron transfer kinetics	585:617	higher electron transfer kinetics	585:617	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	7	54	theme	III	1698:1700	arg1	detection					1682:1690	sensitive detection	1672:1690	sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity	1672:1771	This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface.					
30797108	2	55	theme	biosensing	749:758	arg1	assays					760:765	biosensing assays	749:765	biosensing assays	749:765	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	5	56	theme	As	1380:1381	arg1	aptasensor					1388:1397	an optical As(III) aptasensor	1369:1397	an optical As(III) aptasensor using the UV-Vis spectroscopic method	1369:1435	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	1	57	theme	first	147:151	arg1	time					153:156	the first time	143:156	the first time	143:156	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	4	58	theme	lowest	1083:1088	arg1	limits					1090:1095	the lowest limits	1079:1095	the lowest limits of detection for As(III)	1079:1120	This protocol provided one of the lowest limits of detection for As(III) on aptamer-based electrodes recently described in the literature.					
30797108	1	59	theme	sensitive	382:390	arg1	detection					392:400	highly sensitive detection	375:400	highly sensitive detection of As(III)	375:411	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	6	60	theme	samples	1593:1599	arg1	ions					1552:1555	other metal ions	1540:1555	other metal ions	1540:1555	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	6	60	theme	samples	1593:1599	arg1	matrix					1571:1576	a complex matrix	1561:1576	a complex matrix of waste water samples	1561:1599	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	2	61	theme	Chit-Naf	562:569	arg1	composite					571:579	Chit-Naf composite	562:579	Chit-Naf composite	562:579	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	6	62	theme	waste	1581:1585	arg1	samples					1593:1599	waste water samples	1581:1599	waste water samples	1581:1599	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	3	63	theme	nanotube-bovine	842:856	arg1	albumin					864:870	carbon nanotube-bovine serum albumin	835:870	carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system	835:894	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	3	63	theme	nanotube-bovine	842:856	arg1	CNT-BSA					873:879	CNT-BSA	873:879	CNT-BSA	873:879	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	6	64	theme	matrix	1571:1576	arg1	presence					1528:1535	the presence	1524:1535	the presence of other metal ions and a complex matrix of waste water samples	1524:1599	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	6	65	theme	aptasensor	1486:1495	arg1	capability					1457:1466	The discrimination capability	1438:1466	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples	1438:1599	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	0	66	dep	aptasensor	106:115	arg1	III					101:103	III	101:103	III	101:103	Novel chitosan-Nafion composite for fabrication of highly sensitive impedimetric and colorimetric As(III) aptasensor.					
30797108	3	67	theme	albumin	864:870	arg1	system					889:894	carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system	835:894	carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system	835:894	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	2	68	theme	carbon	516:521	arg1	electrode					538:546	the glassy carbon electrode (GC) electrode	505:546	the glassy carbon electrode (GC) electrode modified with Chit-Naf composite	505:579	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	5	69	theme	spectroscopic	1416:1428	arg1	method					1430:1435	the UV-Vis spectroscopic method	1405:1435	the UV-Vis spectroscopic method	1405:1435	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	3	70	theme	signal	796:801	arg1	amplification					803:815	signal amplification	796:815	a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively	794:1046	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	1	71	theme	impedimetricaptamer-based	338:362	arg1	sensor					364:369	a lable-free impedimetricaptamer-based sensor	325:369	a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III)	325:411	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	7	72	theme	interface	1828:1836	arg1	free					1789:1792	free	1789:1792	free	1789:1792	This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface.					
30797108	6	73	theme	metal	1546:1550	arg1	ions					1552:1555	other metal ions	1540:1555	other metal ions	1540:1555	The discrimination capability of the fabricated aptasensor for recognizing As(III) in the presence of other metal ions and a complex matrix of waste water samples was successfully investigated.					
30797108	1	74	dep	novel	268:272	arg1	CNT-based					274:282	CNT-based	274:282	CNT-based	274:282	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	4	75	theme	aptamer-based	1125:1137	arg1	electrodes					1139:1148	aptamer-based electrodes	1125:1148	aptamer-based electrodes recently described in the literature	1125:1185	This protocol provided one of the lowest limits of detection for As(III) on aptamer-based electrodes recently described in the literature.					
30797108	3	76	theme	hybrid	882:887	arg1	system					889:894	carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system	835:894	carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system	835:894	In this work, we employed a signal amplification strategy based on carbon nanotube-bovine serum albumin (CNT-BSA) hybrid system, by which sensitivity and detection limit of the aptasensor for the detection of As(III) were obtained to be 100.82 Ω nM-1 and a of 74 pM, respectively.					
30797108	7	77	from	advantages	1721:1730	arg1	terms					1735:1739	terms	1735:1739	terms of reproducibility, selectivity	1735:1771	This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface.					
30797108	2	78	theme	electrochemical	418:432	arg1	EIS					458:460	EIS	458:460	EIS	458:460	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	2	78	theme	electrochemical	418:432	arg1	spectroscopy					444:455	electrochemical impedance spectroscopy	418:455	The electrochemical impedance spectroscopy (EIS) investigations	414:476	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	5	79	theme	absorptive	1224:1233	arg1	properties					1235:1244	the optical absorptive properties	1212:1244	the optical absorptive properties of CNTs	1212:1252	Moreover, the change of the optical absorptive properties of CNTs upon biorecognition interactions provides a way to detect the biorecognition process and thus allowed us to design an optical As(III) aptasensor using the UV-Vis spectroscopic method.					
30797108	7	80	with	detection	1682:1690	arg1	advantages					1721:1730	considerable advantages	1708:1730	considerable advantages in terms of reproducibility, selectivity	1708:1771	This protocol provided a new method for sensitive detection of As(III) with considerable advantages in terms of reproducibility, selectivity, being mediator free and regenerability of the sensing interface.					
30797108	0	81	theme	sensitive	58:66	arg1	impedimetric					68:79	highly sensitive impedimetric	51:79	highly sensitive impedimetric	51:79	Novel chitosan-Nafion composite for fabrication of highly sensitive impedimetric and colorimetric As(III) aptasensor.					
30797108	2	82	theme	spectroscopy	444:455	arg1	investigations					463:476	The electrochemical impedance spectroscopy (EIS) investigations	414:476	The electrochemical impedance spectroscopy (EIS) investigations	414:476	The electrochemical impedance spectroscopy (EIS) investigations surprisingly revealed that the glassy carbon electrode (GC) electrode modified with Chit-Naf composite had higher electron transfer kinetics compared the bare GC, GC/Naf and GC/Chit electrodes, which promises a great potential as an efficient platform in construction of biosensing assays.					
30797108	1	83	theme	amplification	291:303	arg1	advantages					171:180	the advantages	167:180	the advantages of chitosan-Nafion (Chit-Naf) composite	167:220	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	83	theme	amplification	291:303	arg1	strategy					305:312	a novel CNT-based signal amplification strategy	266:312	a novel CNT-based signal amplification strategy	266:312	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	84	theme	Chit-Naf	202:209	arg1	composite					212:220	chitosan-Nafion (Chit-Naf) composite	185:220	chitosan-Nafion (Chit-Naf) composite	185:220	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	85	theme	composite	212:220	arg1	advantages					171:180	the advantages	167:180	the advantages of chitosan-Nafion (Chit-Naf) composite	167:220	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	85	theme	composite	212:220	arg1	strategy					305:312	a novel CNT-based signal amplification strategy	266:312	a novel CNT-based signal amplification strategy	266:312	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
30797108	1	85	theme	composite	212:220	arg1	platform					253:260	a highly conductive surface platform	225:260	a highly conductive surface platform	225:260	In the present work, for the first time we takes the advantages of chitosan-Nafion (Chit-Naf) composite as a highly conductive surface platform and a novel CNT-based signal amplification strategy to develop a lable-free impedimetricaptamer-based sensor for highly sensitive detection of As(III).					
31472829	3	0	theme	BSA	433:435	arg1	efficiency					419:428	The encapsulation efficiency	401:428	The encapsulation efficiency of BSA in SDS/Ca-Alg beads	401:455	The encapsulation efficiency of BSA in SDS/Ca-Alg beads was found significantly higher (96.3%) compared to that of beads without SDS.					
31472829	0	1	theme	drug	78:81	arg1	release					59:65	controlled release	48:65	controlled release of protein drug	48:81	Surfactant modified alginate composite gels for controlled release of protein drug.					
31472829	4	2	theme	modified	595:602	arg1	gel					604:606	the modified gel	591:606	the modified gel in the stomach environment	591:633	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	4	3	theme	protein	673:679	arg1	release					681:687	protein release	673:687	protein release from the plain alginate gel	673:715	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	6	4	from	degradation	989:999	arg1	fluid					1019:1023	acidic gastric fluid	1004:1023	acidic gastric fluid	1004:1023	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	3	5	theme	encapsulation	405:417	arg1	efficiency					419:428	The encapsulation efficiency	401:428	The encapsulation efficiency of BSA in SDS/Ca-Alg beads	401:455	The encapsulation efficiency of BSA in SDS/Ca-Alg beads was found significantly higher (96.3%) compared to that of beads without SDS.					
31472829	6	6	theme	suitable	878:885	arg1	carriers					887:894	suitable carriers	878:894	suitable carriers for the passage of orally taken protein-type drugs into the colon medium	878:967	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	6	6	theme	suitable	878:885	arg1	beads					856:860	The SDS-modified alginate beads	830:860	The SDS-modified alginate beads	830:860	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	2	7	theme	model	313:317	arg1	protein					319:325	a model protein	311:325	a model protein	311:325	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	7	theme	model	313:317	arg1	albumin					276:282	Bovine serum albumin	263:282	Bovine serum albumin (BSA)	263:288	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	8	theme	alginate	372:379	arg1	SDS/Ca-Alg					388:397	SDS/Ca-Alg	388:397	SDS/Ca-Alg	388:397	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	8	theme	alginate	372:379	arg1	beads					381:385	surfactant modified calcium alginate beads	344:385	surfactant modified calcium alginate beads (SDS/Ca-Alg)	344:398	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	1	9	with	alginate	110:117	arg1	sulfate					139:145	sodium dodecyl sulfate	124:145	sodium dodecyl sulfate (SDS)	124:151	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	1	9	with	alginate	110:117	arg1	SDS					148:150	SDS	148:150	SDS	148:150	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	3	10	from	efficiency	419:428	arg1	beads					451:455	SDS/Ca-Alg beads	440:455	SDS/Ca-Alg beads	440:455	The encapsulation efficiency of BSA in SDS/Ca-Alg beads was found significantly higher (96.3%) compared to that of beads without SDS.					
31472829	2	11	theme	serum	270:274	arg1	protein					319:325	a model protein	311:325	a model protein	311:325	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	11	theme	serum	270:274	arg1	BSA					285:287	BSA	285:287	BSA	285:287	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	11	theme	serum	270:274	arg1	albumin					276:282	Bovine serum albumin	263:282	Bovine serum albumin (BSA)	263:288	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	4	12	theme	protein	570:576	arg1	release					578:584	protein release	570:584	protein release from the modified gel in the stomach environment	570:633	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	0	13	theme	composite	29:37	arg1	gels					39:42	alginate composite gels	20:42	alginate composite gels for controlled release of protein drug	20:81	Surfactant modified alginate composite gels for controlled release of protein drug.					
31472829	2	14	theme	Bovine	263:268	arg1	protein					319:325	a model protein	311:325	a model protein	311:325	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	14	theme	Bovine	263:268	arg1	BSA					285:287	BSA	285:287	BSA	285:287	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	14	theme	Bovine	263:268	arg1	albumin					276:282	Bovine serum albumin	263:282	Bovine serum albumin (BSA)	263:288	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	15	theme	surfactant	344:353	arg1	SDS/Ca-Alg					388:397	SDS/Ca-Alg	388:397	SDS/Ca-Alg	388:397	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	15	theme	surfactant	344:353	arg1	beads					381:385	surfactant modified calcium alginate beads	344:385	surfactant modified calcium alginate beads (SDS/Ca-Alg)	344:398	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	16	theme	modified	355:362	arg1	SDS/Ca-Alg					388:397	SDS/Ca-Alg	388:397	SDS/Ca-Alg	388:397	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	16	theme	modified	355:362	arg1	beads					381:385	surfactant modified calcium alginate beads	344:385	surfactant modified calcium alginate beads (SDS/Ca-Alg)	344:398	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	1	17	theme	colon	249:253	arg1	medium					255:260	the colon medium	245:260	the colon medium	245:260	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	5	18	from	time	730:733	arg1	time					748:751	the release time	736:751	the release time of the whole drug in the intestinal environment	736:799	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	5	18	from	time	730:733	arg1	prolonged					819:827	prolonged	819:827	prolonged	819:827	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	5	19	theme	intestinal	778:787	arg1	environment					789:799	the intestinal environment	774:799	the intestinal environment	774:799	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	3	20	theme	SDS/Ca-Alg	440:449	arg1	beads					451:455	SDS/Ca-Alg beads	440:455	SDS/Ca-Alg beads	440:455	The encapsulation efficiency of BSA in SDS/Ca-Alg beads was found significantly higher (96.3%) compared to that of beads without SDS.					
31472829	6	21	theme	drugs	941:945	arg1	passage					904:910	the passage	900:910	the passage of orally taken protein-type drugs into the colon medium	900:967	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	5	22	theme	whole	760:764	arg1	drug					766:769	the whole drug	756:769	the whole drug	756:769	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	1	23	theme	oral	178:181	arg1	protein					183:189	oral protein	178:189	oral protein	178:189	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	6	24	theme	alginate	847:854	arg1	carriers					887:894	suitable carriers	878:894	suitable carriers for the passage of orally taken protein-type drugs into the colon medium	878:967	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	6	24	theme	alginate	847:854	arg1	beads					856:860	The SDS-modified alginate beads	830:860	The SDS-modified alginate beads	830:860	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	2	25	theme	calcium	364:370	arg1	SDS/Ca-Alg					388:397	SDS/Ca-Alg	388:397	SDS/Ca-Alg	388:397	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	2	25	theme	calcium	364:370	arg1	beads					381:385	surfactant modified calcium alginate beads	344:385	surfactant modified calcium alginate beads (SDS/Ca-Alg)	344:398	Bovine serum albumin (BSA), which was chosen as a model protein, was loaded into surfactant modified calcium alginate beads (SDS/Ca-Alg).					
31472829	5	26	theme	release	740:746	arg1	time					748:751	the release time	736:751	the release time of the whole drug in the intestinal environment	736:799	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	5	26	theme	release	740:746	arg1	prolonged					819:827	prolonged	819:827	prolonged	819:827	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	1	27	theme	protein	183:189	arg1	release					167:173	the release	163:173	the release of oral protein in the acidic stomach environment	163:223	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	5	28	theme	drug	766:769	arg1	time					748:751	the release time	736:751	the release time of the whole drug in the intestinal environment	736:799	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	5	28	theme	drug	766:769	arg1	prolonged					819:827	prolonged	819:827	prolonged	819:827	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	6	29	theme	protein-type	928:939	arg1	drugs					941:945	orally taken protein-type drugs	915:945	orally taken protein-type drugs	915:945	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	6	30	theme	acidic	1004:1009	arg1	fluid					1019:1023	acidic gastric fluid	1004:1023	acidic gastric fluid	1004:1023	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	0	31	theme	controlled	48:57	arg1	release					59:65	controlled release	48:65	controlled release of protein drug	48:81	Surfactant modified alginate composite gels for controlled release of protein drug.					
31472829	6	32	theme	colon	956:960	arg1	medium					962:967	the colon medium	952:967	the colon medium	952:967	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	1	33	from	release	167:173	arg1	environment					213:223	the acidic stomach environment	194:223	the acidic stomach environment	194:223	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	3	34	dep	found	461:465	arg1	%					493:493	96.3%	489:493	96.3%	489:493	The encapsulation efficiency of BSA in SDS/Ca-Alg beads was found significantly higher (96.3%) compared to that of beads without SDS.					
31472829	4	35	theme	alginate	704:711	arg1	gel					713:715	the plain alginate gel	694:715	the plain alginate gel	694:715	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	4	36	theme	plain	698:702	arg1	gel					713:715	the plain alginate gel	694:715	the plain alginate gel	694:715	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	6	37	theme	gastric	1011:1017	arg1	fluid					1019:1023	acidic gastric fluid	1004:1023	acidic gastric fluid	1004:1023	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	6	38	theme	taken	922:926	arg1	drugs					941:945	orally taken protein-type drugs	915:945	orally taken protein-type drugs	915:945	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	6	39	theme	SDS-modified	834:845	arg1	carriers					887:894	suitable carriers	878:894	suitable carriers for the passage of orally taken protein-type drugs into the colon medium	878:967	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	6	39	theme	SDS-modified	834:845	arg1	beads					856:860	The SDS-modified alginate beads	830:860	The SDS-modified alginate beads	830:860	The SDS-modified alginate beads are proposed as suitable carriers for the passage of orally taken protein-type drugs into the colon medium by preventing their degradation in acidic gastric fluid.					
31472829	5	40	theme	same	725:728	arg1	time					730:733	the same time	721:733	the same time	721:733	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	4	41	from	gel	713:715	arg1	release					681:687	protein release	673:687	protein release from the plain alginate gel	673:715	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	1	42	theme	sodium	124:129	arg1	sulfate					139:145	sodium dodecyl sulfate	124:145	sodium dodecyl sulfate (SDS)	124:151	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	1	42	theme	sodium	124:129	arg1	SDS					148:150	SDS	148:150	SDS	148:150	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	4	43	theme	stomach	615:621	arg1	environment					623:633	the stomach environment	611:633	the stomach environment	611:633	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	0	44	theme	protein	70:76	arg1	drug					78:81	protein drug	70:81	protein drug	70:81	Surfactant modified alginate composite gels for controlled release of protein drug.					
31472829	4	45	theme	remarkable	544:553	arg1	result					555:560	The most remarkable result	535:560	The most remarkable result	535:560	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	1	46	theme	dodecyl	131:137	arg1	sulfate					139:145	sodium dodecyl sulfate	124:145	sodium dodecyl sulfate (SDS)	124:151	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	1	46	theme	dodecyl	131:137	arg1	SDS					148:150	SDS	148:150	SDS	148:150	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	1	47	theme	acidic	198:203	arg1	environment					213:223	the acidic stomach environment	194:223	the acidic stomach environment	194:223	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31472829	0	48	theme	alginate	20:27	arg1	gels					39:42	alginate composite gels	20:42	alginate composite gels for controlled release of protein drug	20:81	Surfactant modified alginate composite gels for controlled release of protein drug.					
31472829	5	49	from	time	748:751	arg1	environment					789:799	the intestinal environment	774:799	the intestinal environment	774:799	At the same time, the release time of the whole drug in the intestinal environment was significantly prolonged.					
31472829	4	50	from	gel	604:606	arg1	environment					623:633	the stomach environment	611:633	the stomach environment	611:633	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	4	50	from	gel	604:606	arg1	release					578:584	protein release	570:584	protein release from the modified gel in the stomach environment	570:633	The most remarkable result is that protein release from the modified gel in the stomach environment was significantly reduced compared to protein release from the plain alginate gel.					
31472829	1	51	theme	stomach	205:211	arg1	environment					213:223	the acidic stomach environment	194:223	the acidic stomach environment	194:223	This study aims to modify alginate with sodium dodecyl sulfate (SDS) to reduce the release of oral protein in the acidic stomach environment and transport it to the colon medium.					
31595890	6	0	theme	silicate	995:1002	arg1	release					1008:1014	silicate ion release	995:1014	silicate ion release	995:1014	The silicate/alginate scaffold could release calcium and silicate ions, and in particular, silicate ion release was highly sustainable for over one week at therapeutically relevant levels.					
31595890	10	1	theme	mineral	1586:1592	arg1	formation					1611:1619	acellular mineral (hydroxyapatite) formation	1576:1619	acellular mineral (hydroxyapatite) formation	1576:1619	The presence of a silicate shell was also shown to accelerate acellular mineral (hydroxyapatite) formation.					
31595890	9	2	theme	silicate-shelled	1450:1465	arg1	scaffolds					1467:1475	the silicate-shelled scaffolds	1446:1475	the silicate-shelled scaffolds	1446:1475	In vivo implantation in subcutaneous tissue revealed more pronounced blood vessel formation around the silicate-shelled scaffolds than around silicate-free scaffolds.					
31595890	12	3	theme	rat	1801:1803	arg1	defect					1815:1820	a rat calvarium defect	1799:1820	a rat calvarium defect	1799:1820	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	5	4	with	layer	863:867	arg1	compositions					890:901	various sol-gel compositions	874:901	various sol-gel compositions	874:901	An alginate hydrogel was injected to form a fiber scaffold with shape-tunability that was then coated with a thin silicate layer with various sol-gel compositions.					
31595890	11	5	theme	osteogenic	1741:1750	arg1	OCN					1775:1777	OCN	1775:1777	OCN	1775:1777	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	11	5	theme	osteogenic	1741:1750	arg1	ALP					1767:1769	ALP	1767:1769	ALP	1767:1769	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	11	5	theme	osteogenic	1741:1750	arg1	Col1a1					1759:1764	Col1a1	1759:1764	Col1a1	1759:1764	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	11	5	theme	osteogenic	1741:1750	arg1	genes					1752:1756	osteogenic genes	1741:1756	osteogenic genes (Col1a1, ALP and OCN)	1741:1778	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	5	6	theme	alginate	743:750	arg1	hydrogel					752:759	An alginate hydrogel	740:759	An alginate hydrogel	740:759	An alginate hydrogel was injected to form a fiber scaffold with shape-tunability that was then coated with a thin silicate layer with various sol-gel compositions.					
31595890	8	7	theme	in	1270:1271	arg1	networking					1287:1296	the in vitro tubular networking	1266:1296	the in vitro tubular networking of cells	1266:1305	Moreover, the in vitro tubular networking of cells was significantly enhanced (1.5 times).					
31595890	9	8	theme	silicate-free	1489:1501	arg1	scaffolds					1503:1511	silicate-free scaffolds	1489:1511	silicate-free scaffolds	1489:1511	In vivo implantation in subcutaneous tissue revealed more pronounced blood vessel formation around the silicate-shelled scaffolds than around silicate-free scaffolds.					
31595890	8	9	theme	tubular	1279:1285	arg1	networking					1287:1296	the in vitro tubular networking	1266:1296	the in vitro tubular networking of cells	1266:1305	Moreover, the in vitro tubular networking of cells was significantly enhanced (1.5 times).					
31595890	7	10	theme	angiogenic	1170:1179	arg1	KDR					1196:1198	KDR	1196:1198	KDR	1196:1198	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	10	theme	angiogenic	1170:1179	arg1	HIF1-α					1217:1222	HIF1-α	1217:1222	HIF1-α	1217:1222	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	10	theme	angiogenic	1170:1179	arg1	markers					1181:1187	angiogenic markers	1170:1187	angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α)	1170:1223	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	10	theme	angiogenic	1170:1179	arg1	eNOS					1201:1204	eNOS	1201:1204	eNOS	1201:1204	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	10	theme	angiogenic	1170:1179	arg1	bFGF					1207:1210	bFGF	1207:1210	bFGF	1207:1210	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	10	theme	angiogenic	1170:1179	arg1	VEGF					1190:1193	VEGF	1190:1193	VEGF	1190:1193	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	4	11	with	scaffold	594:601	arg1	structure					628:636	a hard/soft layered structure	608:636	a hard/soft layered structure	608:636	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	9	12	theme	pronounced	1405:1414	arg1	formation					1429:1437	more pronounced blood vessel formation	1400:1437	more pronounced blood vessel formation	1400:1437	In vivo implantation in subcutaneous tissue revealed more pronounced blood vessel formation around the silicate-shelled scaffolds than around silicate-free scaffolds.					
31595890	4	13	theme	biological	717:726	arg1	properties					728:737	biological properties	717:737	biological properties	717:737	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	13	14	theme	bone	2220:2223	arg1	bioactivity					2225:2235	bone bioactivity	2220:2235	bone bioactivity	2220:2235	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	7	15	theme	ionic	1097:1101	arg1	effective					1122:1130	effective	1122:1130	effective	1122:1130	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	15	theme	ionic	1097:1101	arg1	release					1103:1109	The ionic release	1093:1109	The ionic release	1093:1109	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	13	16	theme	ion-induced	2181:2191	arg1	angiogenesis					2193:2204	ion-induced angiogenesis	2181:2204	ion-induced angiogenesis	2181:2204	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	4	17	from	coating	681:687	arg1	biopolymer					692:701	biopolymer	692:701	biopolymer	692:701	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	1	18	theme	tissues	161:167	arg1	repair					143:148	the repair	139:148	the repair of damaged tissues, including bone	139:183	Promoting angiogenesis is a key strategy for stimulating the repair of damaged tissues, including bone.					
31595890	13	19	theme	hydrogel	2147:2154	arg1	scaffolds					2156:2164	polymeric hydrogel scaffolds	2137:2164	polymeric hydrogel scaffolds	2137:2164	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	12	20	theme	concurrent	1957:1966	arg1	sign					1968:1971	a concurrent sign	1955:1971	a concurrent sign of proangiogenesis	1955:1990	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	6	21	theme	relevant	1076:1083	arg1	levels					1085:1090	therapeutically relevant levels	1060:1090	therapeutically relevant levels	1060:1090	The silicate/alginate scaffold could release calcium and silicate ions, and in particular, silicate ion release was highly sustainable for over one week at therapeutically relevant levels.					
31595890	1	22	theme	Promoting	82:90	arg1	angiogenesis					92:103	Promoting angiogenesis	82:103	Promoting angiogenesis	82:103	Promoting angiogenesis is a key strategy for stimulating the repair of damaged tissues, including bone.					
31595890	1	22	theme	Promoting	82:90	arg1	strategy					114:121	a key strategy	108:121	a key strategy for stimulating the repair of damaged tissues, including bone	108:183	Promoting angiogenesis is a key strategy for stimulating the repair of damaged tissues, including bone.					
31595890	3	23	theme	Silicate-based	356:369	arg1	biomaterials					371:382	Silicate-based biomaterials	356:382	Silicate-based biomaterials	356:382	Silicate-based biomaterials have been reported to induce neovascularization through vascular endothelial growth factor signaling pathway, potentiating acceleration of bone regeneration.					
31595890	13	24	theme	regeneration	2112:2123	arg1	capacity					2125:2132	the bone regeneration capacity	2103:2132	the bone regeneration capacity of polymeric hydrogel scaffolds	2103:2164	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	5	25	theme	thin	849:852	arg1	layer					863:867	a thin silicate layer	847:867	a thin silicate layer with various sol-gel compositions	847:901	An alginate hydrogel was injected to form a fiber scaffold with shape-tunability that was then coated with a thin silicate layer with various sol-gel compositions.					
31595890	6	26	from	levels	1085:1090	arg1	week					1052:1055	one week	1048:1055	one week at therapeutically relevant levels	1048:1090	The silicate/alginate scaffold could release calcium and silicate ions, and in particular, silicate ion release was highly sustainable for over one week at therapeutically relevant levels.					
31595890	3	27	theme	factor	468:473	arg1	pathway					485:491	vascular endothelial growth factor signaling pathway	440:491	vascular endothelial growth factor signaling pathway	440:491	Silicate-based biomaterials have been reported to induce neovascularization through vascular endothelial growth factor signaling pathway, potentiating acceleration of bone regeneration.					
31595890	5	28	theme	sol-gel	882:888	arg1	compositions					890:901	various sol-gel compositions	874:901	various sol-gel compositions	874:901	An alginate hydrogel was injected to form a fiber scaffold with shape-tunability that was then coated with a thin silicate layer with various sol-gel compositions.					
31595890	9	29	from	implantation	1355:1366	arg1	tissue					1384:1389	subcutaneous tissue	1371:1389	subcutaneous tissue	1371:1389	In vivo implantation in subcutaneous tissue revealed more pronounced blood vessel formation around the silicate-shelled scaffolds than around silicate-free scaffolds.					
31595890	1	30	theme	key	110:112	arg1	angiogenesis					92:103	Promoting angiogenesis	82:103	Promoting angiogenesis	82:103	Promoting angiogenesis is a key strategy for stimulating the repair of damaged tissues, including bone.					
31595890	1	30	theme	key	110:112	arg1	strategy					114:121	a key strategy	108:121	a key strategy for stimulating the repair of damaged tissues, including bone	108:183	Promoting angiogenesis is a key strategy for stimulating the repair of damaged tissues, including bone.					
31595890	6	31	theme	silicate	961:968	arg1	ions					970:973	calcium and silicate ions	949:973	calcium and silicate ions	949:973	The silicate/alginate scaffold could release calcium and silicate ions, and in particular, silicate ion release was highly sustainable for over one week at therapeutically relevant levels.					
31595890	3	32	theme	endothelial	449:459	arg1	factor					468:473	vascular endothelial growth factor	440:473	vascular endothelial growth factor signaling pathway	440:491	Silicate-based biomaterials have been reported to induce neovascularization through vascular endothelial growth factor signaling pathway, potentiating acceleration of bone regeneration.					
31595890	4	33	theme	layered	620:626	arg1	structure					628:636	a hard/soft layered structure	608:636	a hard/soft layered structure	608:636	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	10	34	theme	shell	1541:1545	arg1	presence					1518:1525	The presence	1514:1525	The presence of a silicate shell	1514:1545	The presence of a silicate shell was also shown to accelerate acellular mineral (hydroxyapatite) formation.					
31595890	5	35	theme	silicate	854:861	arg1	layer					863:867	a thin silicate layer	847:867	a thin silicate layer with various sol-gel compositions	847:901	An alginate hydrogel was injected to form a fiber scaffold with shape-tunability that was then coated with a thin silicate layer with various sol-gel compositions.					
31595890	0	36	theme	silicate-shelled	39:54	arg1	scaffolds					71:79	silicate-shelled hydrogel fiber scaffolds	39:79	silicate-shelled hydrogel fiber scaffolds	39:79	Angiogenesis-promoted bone repair with silicate-shelled hydrogel fiber scaffolds.					
31595890	6	37	theme	calcium	949:955	arg1	ions					970:973	calcium and silicate ions	949:973	calcium and silicate ions	949:973	The silicate/alginate scaffold could release calcium and silicate ions, and in particular, silicate ion release was highly sustainable for over one week at therapeutically relevant levels.					
31595890	9	38	dep	In	1347:1348	arg1	vivo					1350:1353	vivo	1350:1353	vivo	1350:1353	In vivo implantation in subcutaneous tissue revealed more pronounced blood vessel formation around the silicate-shelled scaffolds than around silicate-free scaffolds.					
31595890	10	39	theme	acellular	1576:1584	arg1	formation					1611:1619	acellular mineral (hydroxyapatite) formation	1576:1619	acellular mineral (hydroxyapatite) formation	1576:1619	The presence of a silicate shell was also shown to accelerate acellular mineral (hydroxyapatite) formation.					
31595890	0	40	theme	fiber	65:69	arg1	scaffolds					71:79	silicate-shelled hydrogel fiber scaffolds	39:79	silicate-shelled hydrogel fiber scaffolds	39:79	Angiogenesis-promoted bone repair with silicate-shelled hydrogel fiber scaffolds.					
31595890	6	41	from	sustainable	1027:1037	arg1	particular					983:992	particular	983:992	particular	983:992	The silicate/alginate scaffold could release calcium and silicate ions, and in particular, silicate ion release was highly sustainable for over one week at therapeutically relevant levels.					
31595890	2	42	theme	therapeutic	337:347	arg1	doses					349:353	therapeutic doses	337:353	therapeutic doses	337:353	Among other proangiogenic factors, ions have recently been considered a potent element that can be incorporated into biomaterials and then released at therapeutic doses.					
31595890	9	43	theme	blood	1416:1420	arg1	formation					1429:1437	more pronounced blood vessel formation	1400:1437	more pronounced blood vessel formation	1400:1437	In vivo implantation in subcutaneous tissue revealed more pronounced blood vessel formation around the silicate-shelled scaffolds than around silicate-free scaffolds.					
31595890	6	44	theme	silicate/alginate	908:924	arg1	scaffold					926:933	The silicate/alginate scaffold	904:933	The silicate/alginate scaffold	904:933	The silicate/alginate scaffold could release calcium and silicate ions, and in particular, silicate ion release was highly sustainable for over one week at therapeutically relevant levels.					
31595890	12	45	theme	silicate-shelled	1827:1842	arg1	scaffold					1844:1851	the silicate-shelled scaffold	1823:1851	the silicate-shelled scaffold	1823:1851	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	2	46	theme	proangiogenic	198:210	arg1	factors					212:218	other proangiogenic factors	192:218	other proangiogenic factors	192:218	Among other proangiogenic factors, ions have recently been considered a potent element that can be incorporated into biomaterials and then released at therapeutic doses.					
31595890	13	47	theme	composition	2052:2062	arg1	approach					2080:2087	an effective approach	2067:2087	an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface	2067:2250	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	13	47	theme	composition	2052:2062	arg1	surface-layering					2023:2038	the surface-layering	2019:2038	the surface-layering of silicate composition	2019:2062	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	6	48	theme	ion	1004:1006	arg1	release					1008:1014	silicate ion release	995:1014	silicate ion release	995:1014	The silicate/alginate scaffold could release calcium and silicate ions, and in particular, silicate ion release was highly sustainable for over one week at therapeutically relevant levels.					
31595890	4	49	theme	hydrogel	579:586	arg1	scaffold					594:601	a silicate-shelled hydrogel fiber scaffold	560:601	a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure	560:636	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	11	50	theme	enhanced	1718:1725	arg1	expression					1727:1736	the enhanced expression	1714:1736	the enhanced expression of osteogenic genes (Col1a1, ALP and OCN)	1714:1778	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	11	51	theme	scaffold	1683:1690	arg1	potential					1648:1656	The cellular osteogenesis potential	1622:1656	The cellular osteogenesis potential of the silicate/alginate scaffold	1622:1690	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	12	52	theme	calvarium	1805:1813	arg1	defect					1815:1820	a rat calvarium defect	1799:1820	a rat calvarium defect	1799:1820	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	7	53	from	expression	1156:1165	arg1	HUVECs					1247:1252	HUVECs	1247:1252	HUVECs	1247:1252	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	53	from	expression	1156:1165	arg1	cells					1240:1244	endothelial cells	1228:1244	endothelial cells (HUVECs)	1228:1253	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	11	54	theme	genes	1752:1756	arg1	expression					1727:1736	the enhanced expression	1714:1736	the enhanced expression of osteogenic genes (Col1a1, ALP and OCN)	1714:1778	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	7	55	theme	markers	1181:1187	arg1	expression					1156:1165	the mRNA expression	1147:1165	the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs)	1147:1253	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	11	56	theme	cellular	1626:1633	arg1	potential					1648:1656	The cellular osteogenesis potential	1622:1656	The cellular osteogenesis potential of the silicate/alginate scaffold	1622:1690	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	7	57	theme	mRNA	1151:1154	arg1	expression					1156:1165	the mRNA expression	1147:1165	the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs)	1147:1253	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	5	58	theme	fiber	784:788	arg1	scaffold					790:797	a fiber scaffold	782:797	a fiber scaffold	782:797	An alginate hydrogel was injected to form a fiber scaffold with shape-tunability that was then coated with a thin silicate layer with various sol-gel compositions.					
31595890	4	59	from	biopolymer	692:701	arg1	possibility					657:667	the possibility	653:667	the possibility of silicate coating on biopolymer for enhancing biological properties	653:737	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	8	60	theme	cells	1301:1305	arg1	networking					1287:1296	the in vitro tubular networking	1266:1296	the in vitro tubular networking of cells	1266:1305	Moreover, the in vitro tubular networking of cells was significantly enhanced (1.5 times).					
31595890	7	61	dep	markers	1181:1187	arg1	KDR					1196:1198	KDR	1196:1198	KDR	1196:1198	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	61	dep	markers	1181:1187	arg1	HIF1-α					1217:1222	HIF1-α	1217:1222	HIF1-α	1217:1222	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	61	dep	markers	1181:1187	arg1	markers					1181:1187	angiogenic markers	1170:1187	angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α)	1170:1223	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	61	dep	markers	1181:1187	arg1	eNOS					1201:1204	eNOS	1201:1204	eNOS	1201:1204	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	61	dep	markers	1181:1187	arg1	bFGF					1207:1210	bFGF	1207:1210	bFGF	1207:1210	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	61	dep	markers	1181:1187	arg1	VEGF					1190:1193	VEGF	1190:1193	VEGF	1190:1193	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	3	62	theme	regeneration	528:539	arg1	acceleration					507:518	acceleration	507:518	acceleration of bone regeneration	507:539	Silicate-based biomaterials have been reported to induce neovascularization through vascular endothelial growth factor signaling pathway, potentiating acceleration of bone regeneration.					
31595890	2	63	theme	potent	258:263	arg1	element					265:271	a potent element	256:271	a potent element that can be incorporated into biomaterials and then released at therapeutic doses	256:353	Among other proangiogenic factors, ions have recently been considered a potent element that can be incorporated into biomaterials and then released at therapeutic doses.					
31595890	1	64	theme	damaged	153:159	arg1	tissues					161:167	damaged tissues	153:167	damaged tissues	153:167	Promoting angiogenesis is a key strategy for stimulating the repair of damaged tissues, including bone.					
31595890	1	64	theme	damaged	153:159	arg1	bone					180:183	bone	180:183	bone	180:183	Promoting angiogenesis is a key strategy for stimulating the repair of damaged tissues, including bone.					
31595890	13	65	theme	polymeric	2137:2145	arg1	scaffolds					2156:2164	polymeric hydrogel scaffolds	2137:2164	polymeric hydrogel scaffolds	2137:2164	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	4	66	theme	silicate-shelled	562:577	arg1	scaffold					594:601	a silicate-shelled hydrogel fiber scaffold	560:601	a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure	560:636	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	4	67	from	possibility	657:667	arg1	biopolymer					692:701	biopolymer	692:701	biopolymer	692:701	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	13	68	theme	scaffolds	2156:2164	arg1	capacity					2125:2132	the bone regeneration capacity	2103:2132	the bone regeneration capacity of polymeric hydrogel scaffolds	2103:2164	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	12	69	theme	proangiogenesis	1976:1990	arg1	sign					1968:1971	a concurrent sign	1955:1971	a concurrent sign of proangiogenesis	1955:1990	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	12	70	dep	demonstrated	1853:1864	arg1	improved					1880:1887	improved	1880:1887	demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis	1853:1990	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	7	71	theme	endothelial	1228:1238	arg1	HUVECs					1247:1252	HUVECs	1247:1252	HUVECs	1247:1252	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	7	71	theme	endothelial	1228:1238	arg1	cells					1240:1244	endothelial cells	1228:1244	endothelial cells (HUVECs)	1228:1253	The ionic release was highly effective in stimulating the mRNA expression of angiogenic markers (VEGF, KDR, eNOS, bFGF, and HIF1-α) in endothelial cells (HUVECs).					
31595890	13	72	theme	bone	2107:2110	arg1	capacity					2125:2132	the bone regeneration capacity	2103:2132	the bone regeneration capacity of polymeric hydrogel scaffolds	2103:2164	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	5	73	theme	various	874:880	arg1	compositions					890:901	various sol-gel compositions	874:901	various sol-gel compositions	874:901	An alginate hydrogel was injected to form a fiber scaffold with shape-tunability that was then coated with a thin silicate layer with various sol-gel compositions.					
31595890	9	74	theme	In	1347:1348	arg1	implantation					1355:1366	In vivo implantation	1347:1366	In vivo implantation in subcutaneous tissue	1347:1389	In vivo implantation in subcutaneous tissue revealed more pronounced blood vessel formation around the silicate-shelled scaffolds than around silicate-free scaffolds.					
31595890	4	75	theme	hard/soft	610:618	arg1	structure					628:636	a hard/soft layered structure	608:636	a hard/soft layered structure	608:636	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	3	76	theme	signaling	475:483	arg1	pathway					485:491	vascular endothelial growth factor signaling pathway	440:491	vascular endothelial growth factor signaling pathway	440:491	Silicate-based biomaterials have been reported to induce neovascularization through vascular endothelial growth factor signaling pathway, potentiating acceleration of bone regeneration.					
31595890	12	77	theme	bone	1925:1928	arg1	volume					1930:1935	bone volume	1925:1935	bone volume	1925:1935	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	8	78	dep	in	1270:1271	arg1	vitro					1273:1277	vitro	1273:1277	vitro	1273:1277	Moreover, the in vitro tubular networking of cells was significantly enhanced (1.5 times).					
31595890	2	79	theme	other	192:196	arg1	factors					212:218	other proangiogenic factors	192:218	other proangiogenic factors	192:218	Among other proangiogenic factors, ions have recently been considered a potent element that can be incorporated into biomaterials and then released at therapeutic doses.					
31595890	12	80	dep	improved	1880:1887	arg1	times					1909:1913	2-3 times	1905:1913	2-3 times higher in bone volume and density	1905:1947	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	3	81	theme	bone	523:526	arg1	regeneration					528:539	bone regeneration	523:539	bone regeneration	523:539	Silicate-based biomaterials have been reported to induce neovascularization through vascular endothelial growth factor signaling pathway, potentiating acceleration of bone regeneration.					
31595890	0	82	theme	hydrogel	56:63	arg1	scaffolds					71:79	silicate-shelled hydrogel fiber scaffolds	39:79	silicate-shelled hydrogel fiber scaffolds	39:79	Angiogenesis-promoted bone repair with silicate-shelled hydrogel fiber scaffolds.					
31595890	9	83	theme	subcutaneous	1371:1382	arg1	tissue					1384:1389	subcutaneous tissue	1371:1389	subcutaneous tissue	1371:1389	In vivo implantation in subcutaneous tissue revealed more pronounced blood vessel formation around the silicate-shelled scaffolds than around silicate-free scaffolds.					
31595890	3	84	theme	growth	461:466	arg1	factor					468:473	vascular endothelial growth factor	440:473	vascular endothelial growth factor signaling pathway	440:491	Silicate-based biomaterials have been reported to induce neovascularization through vascular endothelial growth factor signaling pathway, potentiating acceleration of bone regeneration.					
31595890	4	85	theme	fiber	588:592	arg1	scaffold					594:601	a silicate-shelled hydrogel fiber scaffold	560:601	a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure	560:636	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	10	86	theme	silicate	1532:1539	arg1	shell					1541:1545	a silicate shell	1530:1545	a silicate shell	1530:1545	The presence of a silicate shell was also shown to accelerate acellular mineral (hydroxyapatite) formation.					
31595890	13	87	theme	effective	2070:2078	arg1	approach					2080:2087	an effective approach	2067:2087	an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface	2067:2250	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	13	87	theme	effective	2070:2078	arg1	surface-layering					2023:2038	the surface-layering	2019:2038	the surface-layering of silicate composition	2019:2062	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	12	88	theme	2-3	1905:1907	arg1	times					1909:1913	2-3 times	1905:1913	2-3 times higher in bone volume and density	1905:1947	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	6	89	from	particular	983:992	arg1	sustainable					1027:1037	sustainable	1027:1037	sustainable	1027:1037	The silicate/alginate scaffold could release calcium and silicate ions, and in particular, silicate ion release was highly sustainable for over one week at therapeutically relevant levels.					
31595890	3	90	theme	vascular	440:447	arg1	factor					468:473	vascular endothelial growth factor	440:473	vascular endothelial growth factor signaling pathway	440:491	Silicate-based biomaterials have been reported to induce neovascularization through vascular endothelial growth factor signaling pathway, potentiating acceleration of bone regeneration.					
31595890	11	91	theme	osteogenesis	1635:1646	arg1	potential					1648:1656	The cellular osteogenesis potential	1622:1656	The cellular osteogenesis potential of the silicate/alginate scaffold	1622:1690	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	11	92	dep	genes	1752:1756	arg1	OCN					1775:1777	OCN	1775:1777	OCN	1775:1777	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	11	92	dep	genes	1752:1756	arg1	ALP					1767:1769	ALP	1767:1769	ALP	1767:1769	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	11	92	dep	genes	1752:1756	arg1	Col1a1					1759:1764	Col1a1	1759:1764	Col1a1	1759:1764	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	11	92	dep	genes	1752:1756	arg1	genes					1752:1756	osteogenic genes	1741:1756	osteogenic genes (Col1a1, ALP and OCN)	1741:1778	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	12	93	theme	higher	1915:1920	arg1	times					1909:1913	2-3 times	1905:1913	2-3 times higher in bone volume and density	1905:1947	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	12	94	theme	bone	1889:1892	arg1	formation					1894:1902	bone formation	1889:1902	bone formation	1889:1902	When implanted in a rat calvarium defect, the silicate-shelled scaffold demonstrated significantly improved bone formation (2-3 times higher in bone volume and density) with a concurrent sign of proangiogenesis.					
31595890	4	95	theme	silicate	672:679	arg1	coating					681:687	silicate coating	672:687	silicate coating on biopolymer	672:701	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	13	96	theme	silicate	2043:2050	arg1	composition					2052:2062	silicate composition	2043:2062	silicate composition	2043:2062	This work highlights that the surface-layering of silicate composition is an effective approach for improving the bone regeneration capacity of polymeric hydrogel scaffolds by stimulating ion-induced angiogenesis and providing bone bioactivity to the surface.					
31595890	9	97	theme	vessel	1422:1427	arg1	formation					1429:1437	more pronounced blood vessel formation	1400:1437	more pronounced blood vessel formation	1400:1437	In vivo implantation in subcutaneous tissue revealed more pronounced blood vessel formation around the silicate-shelled scaffolds than around silicate-free scaffolds.					
31595890	8	98	dep	enhanced	1325:1332	arg1	times					1339:1343	1.5 times	1335:1343	1.5 times	1335:1343	Moreover, the in vitro tubular networking of cells was significantly enhanced (1.5 times).					
31595890	10	99	theme	hydroxyapatite	1595:1608	arg1	formation					1611:1619	acellular mineral (hydroxyapatite) formation	1576:1619	acellular mineral (hydroxyapatite) formation	1576:1619	The presence of a silicate shell was also shown to accelerate acellular mineral (hydroxyapatite) formation.					
31595890	4	100	theme	coating	681:687	arg1	possibility					657:667	the possibility	653:667	the possibility of silicate coating on biopolymer for enhancing biological properties	653:737	Here, we designed a silicate-shelled hydrogel fiber scaffold with a hard/soft layered structure to investigate the possibility of silicate coating on biopolymer for enhancing biological properties.					
31595890	11	101	theme	silicate/alginate	1665:1681	arg1	scaffold					1683:1690	the silicate/alginate scaffold	1661:1690	the silicate/alginate scaffold	1661:1690	The cellular osteogenesis potential of the silicate/alginate scaffold was further proven by the enhanced expression of osteogenic genes (Col1a1, ALP and OCN).					
31595890	0	102	theme	bone	22:25	arg1	repair					27:32	bone repair	22:32	bone repair	22:32	Angiogenesis-promoted bone repair with silicate-shelled hydrogel fiber scaffolds.					
30685477	10	0	theme	delivery	1979:1986	arg1	system					1988:1993	a well-established alginate-based delivery system	1945:1993	a well-established alginate-based delivery system	1945:1993	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	13	1	theme	osteoinductive	2408:2421	arg1	factor					2430:2435	an osteoinductive growth factor	2405:2435	an osteoinductive growth factor	2405:2435	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	13	1	theme	osteoinductive	2408:2421	arg1	BMP-2					2438:2442	BMP-2	2438:2442	BMP-2	2438:2442	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	14	2	theme	BMP-2	2524:2528	arg1	vehicle					2539:2545	a BMP-2 delivery vehicle	2522:2545	a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model	2522:2617	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	14	2	theme	BMP-2	2524:2528	arg1	collagen + DCN					2504:2517	collagen + DCN	2504:2517	collagen + DCN	2504:2517	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	7	3	theme	TGF-ß	1481:1485	arg1	factors					1494:1500	TGF-ß growth factors	1481:1500	TGF-ß growth factors	1481:1500	We hypothesized that collagen + DCN would increase BMP-2 retention over collagen alone due to DCN's ability to sequester TGF-ß growth factors.					
30685477	3	4	theme	bone	576:579	arg1	process					589:595	the bone healing process	572:595	the bone healing process	572:595	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	1	5	theme	surrounding	298:308	arg1	tissue					315:320	the surrounding soft tissue	294:320	the surrounding soft tissue	294:320	Traumatic musculoskeletal injuries that result in bone defects or fractures often affect both bone and the surrounding soft tissue.					
30685477	2	6	theme	disability	424:433	arg1	rates					387:391	increased rates	377:391	increased rates of complications and long-term disability	377:433	Clinically, these types of multi-tissue injuries have increased rates of complications and long-term disability.					
30685477	4	7	theme	impaired	700:707	arg1	healing					714:720	impaired bone healing	700:720	impaired bone healing	700:720	Our lab has previously established a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing; however, the vascularization response in this model had not yet been investigated.					
30685477	6	8	theme	morphogenetic	1288:1300	arg1	factor					1352:1357	a potent osteoinductive growth factor	1321:1357	a potent osteoinductive growth factor	1321:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	8	theme	morphogenetic	1288:1300	arg1	BMP-2					1313:1317	BMP-2	1313:1317	BMP-2	1313:1317	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	8	theme	morphogenetic	1288:1300	arg1	protein-2					1302:1310	bone morphogenetic protein-2	1283:1310	bone morphogenetic protein-2 (BMP-2)	1283:1318	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	13	9	theme	delivery	2335:2342	arg1	hydrogel					2285:2292	A decorin-supplemented collagen hydrogel	2253:2292	A decorin-supplemented collagen hydrogel	2253:2292	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	13	9	theme	delivery	2335:2342	arg1	vehicle					2344:2350	a delivery vehicle	2333:2350	a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2	2333:2442	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	4	10	theme	bone-muscle	667:677	arg1	injury					679:684	composite bone-muscle injury	657:684	composite bone-muscle injury	657:684	Our lab has previously established a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing; however, the vascularization response in this model had not yet been investigated.					
30685477	0	11	theme	bone-muscle	135:145	arg1	model					154:158	a composite bone-muscle injury model	123:158	a composite bone-muscle injury model with impaired vascularization	123:188	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	3	12	theme	process	589:595	arg1	step					564:567	a critical early step	547:567	a critical early step of the bone healing process	547:595	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	12	theme	process	589:595	arg1	indicator					473:481	a key clinical indicator	458:481	a key clinical indicator of injury severity	458:500	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	12	theme	process	589:595	arg1	integrity					445:453	Vascular integrity	436:453	Vascular integrity	436:453	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	12	theme	process	589:595	arg1	revascularization					507:523	revascularization	507:523	revascularization of the injury site	507:542	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	15	13	theme	angiogenesis	2651:2662	arg1	support					2633:2639	its support	2629:2639	its support of robust angiogenesis in vitro	2629:2671	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	5	14	theme	composite	867:875	arg1	injury					877:882	composite injury	867:882	composite injury	867:882	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	6	15	theme	bone	1283:1286	arg1	factor					1352:1357	a potent osteoinductive growth factor	1321:1357	a potent osteoinductive growth factor	1321:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	15	theme	bone	1283:1286	arg1	BMP-2					1313:1317	BMP-2	1313:1317	BMP-2	1313:1317	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	15	theme	bone	1283:1286	arg1	protein-2					1302:1310	bone morphogenetic protein-2	1283:1310	bone morphogenetic protein-2 (BMP-2)	1283:1318	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	15	16	with	use	2716:2718	arg1	networks					2781:2788	pre-formed vascular networks	2761:2788	pre-formed vascular networks	2761:2788	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	15	16	with	use	2716:2718	arg1	therapeutics					2740:2751	other vascular therapeutics	2725:2751	other vascular therapeutics such as pre-formed vascular networks	2725:2788	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	0	17	theme	impaired	165:172	arg1	vascularization					174:188	impaired vascularization	165:188	impaired vascularization	165:188	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	6	18	theme	mature	1259:1264	arg1	vasculature					1266:1276	mature vasculature	1259:1276	mature vasculature	1259:1276	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	7	19	theme	BMP-2	1411:1415	arg1	retention					1417:1425	BMP-2 retention	1411:1425	BMP-2 retention	1411:1425	We hypothesized that collagen + DCN would increase BMP-2 retention over collagen alone due to DCN's ability to sequester TGF-ß growth factors.					
30685477	0	20	with	model	154:158	arg1	vascularization					174:188	impaired vascularization	165:188	impaired vascularization	165:188	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	6	21	theme	osteoinductive	1330:1343	arg1	factor					1352:1357	a potent osteoinductive growth factor	1321:1357	a potent osteoinductive growth factor	1321:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	21	theme	osteoinductive	1330:1343	arg1	protein-2					1302:1310	bone morphogenetic protein-2	1283:1310	bone morphogenetic protein-2 (BMP-2)	1283:1318	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	11	22	theme	musculoskeletal	2065:2079	arg1	trauma					2081:2086	musculoskeletal trauma	2065:2086	musculoskeletal trauma	2065:2086	STATEMENT OF SIGNIFICANCE: We have previously established a model of musculoskeletal trauma that exhibits impaired bone healing.					
30685477	5	23	theme	combined	955:962	arg1	vascularization					964:978	combined vascularization	955:978	combined vascularization	955:978	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	12	24	theme	first	2133:2137	arg1	time					2139:2142	the first time	2129:2142	the first time	2129:2142	For the first time, this work shows that the early revascularization response is also significantly, albeit modestly, impaired.					
30685477	0	25	theme	morphogenetic	68:80	arg1	protein-2					82:90	bone morphogenetic protein-2	63:90	bone morphogenetic protein-2	63:90	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	10	26	theme	composite	1881:1889	arg1	model					1898:1902	the challenging composite defect model	1865:1902	the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system	1865:1993	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	10	26	theme	composite	1881:1889	arg1	comparable					1912:1921	comparable	1912:1921	comparable	1912:1921	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	15	27	theme	vascular	2731:2738	arg1	networks					2781:2788	pre-formed vascular networks	2761:2788	pre-formed vascular networks	2761:2788	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	15	27	theme	vascular	2731:2738	arg1	therapeutics					2740:2751	other vascular therapeutics	2725:2751	other vascular therapeutics such as pre-formed vascular networks	2725:2788	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	2	28	contain	have	372:375	arg1	types					341:345	these types	335:345	these types of multi-tissue injuries	335:370	Clinically, these types of multi-tissue injuries have increased rates of complications and long-term disability.					
30685477	2	28	contain	have	372:375	arg2	rates					387:391	increased rates	377:391	increased rates of complications and long-term disability	377:433	Clinically, these types of multi-tissue injuries have increased rates of complications and long-term disability.					
30685477	13	29	used	used	2298:2301	arg2	hydrogel					2285:2292	A decorin-supplemented collagen hydrogel	2253:2292	A decorin-supplemented collagen hydrogel	2253:2292	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	13	29	used	used	2298:2301	arg2	vehicle					2344:2350	a delivery vehicle	2333:2350	a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2	2333:2442	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	1	30	theme	Traumatic	191:199	arg1	injuries					217:224	Traumatic musculoskeletal injuries	191:224	Traumatic musculoskeletal injuries that result in bone defects or fractures	191:265	Traumatic musculoskeletal injuries that result in bone defects or fractures often affect both bone and the surrounding soft tissue.					
30685477	8	31	theme	early	1556:1560	arg1	vascularization					1562:1576	early vascularization	1556:1576	early vascularization	1556:1576	We further hypothesized that MVF would increase both early vascularization and subsequent BMP-2-mediated bone regeneration.					
30685477	0	32	theme	fragments	110:118	arg1	co-delivery					48:58	the co-delivery	44:58	the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization	44:188	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	10	33	theme	BMP-2	1809:1813	arg1	collagen + DCN					1769:1782	collagen + DCN	1769:1782	collagen + DCN	1769:1782	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	10	33	theme	BMP-2	1809:1813	arg1	vehicle					1824:1830	a BMP-2 delivery vehicle	1807:1830	a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system	1807:1993	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	9	34	contain	had	1729:1731	arg2	effect					1736:1741	no effect	1733:1741	no effect	1733:1741	Contrary to our hypothesis, BMP + MVF decreased the number of blood vessels relative to BMP alone and had no effect on bone healing.					
30685477	9	34	contain	had	1729:1731	arg1	BMP + MVF					1655:1663	BMP + MVF	1655:1663	BMP + MVF	1655:1663	Contrary to our hypothesis, BMP + MVF decreased the number of blood vessels relative to BMP alone and had no effect on bone healing.					
30685477	15	35	theme	future	2709:2714	arg1	use					2716:2718	future use	2709:2718	future use with other vascular therapeutics such as pre-formed vascular networks	2709:2788	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	8	36	theme	BMP-2-mediated	1593:1606	arg1	regeneration					1613:1624	subsequent BMP-2-mediated bone regeneration	1582:1624	subsequent BMP-2-mediated bone regeneration	1582:1624	We further hypothesized that MVF would increase both early vascularization and subsequent BMP-2-mediated bone regeneration.					
30685477	3	37	theme	clinical	464:471	arg1	indicator					473:481	a key clinical indicator	458:481	a key clinical indicator of injury severity	458:500	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	37	theme	clinical	464:471	arg1	integrity					445:453	Vascular integrity	436:453	Vascular integrity	436:453	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	0	38	theme	Decorin-supplemented	0:19	arg1	collagen					21:28	Decorin-supplemented collagen	0:28	Decorin-supplemented collagen	0:28	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	1	39	theme	soft	310:313	arg1	tissue					315:320	the surrounding soft tissue	294:320	the surrounding soft tissue	294:320	Traumatic musculoskeletal injuries that result in bone defects or fractures often affect both bone and the surrounding soft tissue.					
30685477	11	40	theme	impaired	2102:2109	arg1	healing					2116:2122	impaired bone healing	2102:2122	impaired bone healing	2102:2122	STATEMENT OF SIGNIFICANCE: We have previously established a model of musculoskeletal trauma that exhibits impaired bone healing.					
30685477	6	41	theme	collagen	1106:1113	arg1	hydrogel					1115:1122	-supplemented collagen hydrogel	1092:1122	-supplemented collagen hydrogel	1092:1122	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	41	theme	collagen	1106:1113	arg1	vehicle					1164:1170	a biomaterial delivery vehicle	1141:1170	a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor	1141:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	41	theme	collagen	1106:1113	arg1	decorin					1079:1085	A decorin	1077:1085	A decorin	1077:1085	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	42	dep	developed	1128:1136	arg1	DCN					1088:1090	DCN	1088:1090	DCN	1088:1090	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	11	43	theme	SIGNIFICANCE	2009:2020	arg1	STATEMENT					1996:2004	STATEMENT	1996:2004	STATEMENT OF SIGNIFICANCE	1996:2020	STATEMENT OF SIGNIFICANCE: We have previously established a model of musculoskeletal trauma that exhibits impaired bone healing.					
30685477	5	44	theme	defect	850:855	arg1	revascularization					822:838	the early revascularization	812:838	the early revascularization of a bone defect following composite injury	812:882	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	5	44	theme	defect	850:855	arg1	impaired					899:906	impaired	899:906	impaired	899:906	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	14	45	theme	defect	2606:2611	arg1	model					2613:2617	the challenging composite defect model	2580:2617	the challenging composite defect model	2580:2617	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	3	46	theme	severity	493:500	arg1	step					564:567	a critical early step	547:567	a critical early step of the bone healing process	547:595	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	46	theme	severity	493:500	arg1	indicator					473:481	a key clinical indicator	458:481	a key clinical indicator of injury severity	458:500	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	46	theme	severity	493:500	arg1	integrity					445:453	Vascular integrity	436:453	Vascular integrity	436:453	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	46	theme	severity	493:500	arg1	revascularization					507:523	revascularization	507:523	revascularization of the injury site	507:542	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	14	47	theme	challenging	2584:2594	arg1	model					2613:2617	the challenging composite defect model	2580:2617	the challenging composite defect model	2580:2617	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	14	48	theme	bone	2490:2493	arg1	healing					2495:2501	bone healing	2490:2501	bone healing	2490:2501	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	3	49	theme	injury	532:537	arg1	site					539:542	the injury site	528:542	the injury site	528:542	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	15	50	theme	pre-formed	2761:2770	arg1	networks					2781:2788	pre-formed vascular networks	2761:2788	pre-formed vascular networks	2761:2788	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	2	51	theme	injuries	363:370	arg1	types					341:345	these types	335:345	these types of multi-tissue injuries	335:370	Clinically, these types of multi-tissue injuries have increased rates of complications and long-term disability.					
30685477	15	52	theme	robust	2644:2649	arg1	angiogenesis					2651:2662	robust angiogenesis	2644:2662	robust angiogenesis	2644:2662	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	9	53	theme	vessels	1695:1701	arg1	number					1679:1684	the number	1675:1684	the number of blood vessels	1675:1701	Contrary to our hypothesis, BMP + MVF decreased the number of blood vessels relative to BMP alone and had no effect on bone healing.					
30685477	6	54	dep	co-delivery	1180:1190	arg1	MVF					1217:1219	MVF	1217:1219	MVF	1217:1219	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	54	dep	co-delivery	1180:1190	arg1	fragments					1206:1214	microvascular fragments	1192:1214	microvascular fragments (MVF)	1192:1220	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	3	55	theme	critical	549:556	arg1	step					564:567	a critical early step	547:567	a critical early step of the bone healing process	547:595	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	55	theme	critical	549:556	arg1	revascularization					507:523	revascularization	507:523	revascularization of the injury site	507:542	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	13	56	theme	decorin-supplemented	2255:2274	arg1	hydrogel					2285:2292	A decorin-supplemented collagen hydrogel	2253:2292	A decorin-supplemented collagen hydrogel	2253:2292	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	13	56	theme	decorin-supplemented	2255:2274	arg1	vehicle					2344:2350	a delivery vehicle	2333:2350	a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2	2333:2442	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	0	57	theme	bone	63:66	arg1	protein-2					82:90	bone morphogenetic protein-2	63:90	bone morphogenetic protein-2	63:90	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	14	58	theme	capable	2547:2553	arg1	vehicle					2539:2545	a BMP-2 delivery vehicle	2522:2545	a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model	2522:2617	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	14	58	theme	capable	2547:2553	arg1	collagen + DCN					2504:2517	collagen + DCN	2504:2517	collagen + DCN	2504:2517	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	5	59	theme	composite	1057:1065	arg1	injuries					1067:1074	composite injuries	1057:1074	composite injuries	1057:1074	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	6	60	theme	biomaterial	1143:1153	arg1	hydrogel					1115:1122	-supplemented collagen hydrogel	1092:1122	-supplemented collagen hydrogel	1092:1122	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	60	theme	biomaterial	1143:1153	arg1	vehicle					1164:1170	a biomaterial delivery vehicle	1141:1170	a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor	1141:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	60	theme	biomaterial	1143:1153	arg1	decorin					1079:1085	A decorin	1077:1085	A decorin	1077:1085	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	15	61	theme	other	2725:2729	arg1	networks					2781:2788	pre-formed vascular networks	2761:2788	pre-formed vascular networks	2761:2788	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	15	61	theme	other	2725:2729	arg1	therapeutics					2740:2751	other vascular therapeutics	2725:2751	other vascular therapeutics such as pre-formed vascular networks	2725:2788	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	6	62	theme	potent	1323:1328	arg1	factor					1352:1357	a potent osteoinductive growth factor	1321:1357	a potent osteoinductive growth factor	1321:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	62	theme	potent	1323:1328	arg1	protein-2					1302:1310	bone morphogenetic protein-2	1283:1310	bone morphogenetic protein-2 (BMP-2)	1283:1318	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	14	63	theme	delivery	2530:2537	arg1	vehicle					2539:2545	a BMP-2 delivery vehicle	2522:2545	a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model	2522:2617	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	14	63	theme	delivery	2530:2537	arg1	collagen + DCN					2504:2517	collagen + DCN	2504:2517	collagen + DCN	2504:2517	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	10	64	theme	alginate-based	1964:1977	arg1	system					1988:1993	a well-established alginate-based delivery system	1945:1993	a well-established alginate-based delivery system	1945:1993	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	10	65	theme	delivery	1815:1822	arg1	collagen + DCN					1769:1782	collagen + DCN	1769:1782	collagen + DCN	1769:1782	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	10	65	theme	delivery	1815:1822	arg1	vehicle					1824:1830	a BMP-2 delivery vehicle	1807:1830	a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system	1807:1993	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	7	66	theme	growth	1487:1492	arg1	factors					1494:1500	TGF-ß growth factors	1481:1500	TGF-ß growth factors	1481:1500	We hypothesized that collagen + DCN would increase BMP-2 retention over collagen alone due to DCN's ability to sequester TGF-ß growth factors.					
30685477	2	67	theme	increased	377:385	arg1	rates					387:391	increased rates	377:391	increased rates of complications and long-term disability	377:433	Clinically, these types of multi-tissue injuries have increased rates of complications and long-term disability.					
30685477	11	68	theme	trauma	2081:2086	arg1	model					2056:2060	a model	2054:2060	a model of musculoskeletal trauma that exhibits impaired bone healing	2054:2122	STATEMENT OF SIGNIFICANCE: We have previously established a model of musculoskeletal trauma that exhibits impaired bone healing.					
30685477	1	69	theme	musculoskeletal	201:215	arg1	injuries					217:224	Traumatic musculoskeletal injuries	191:224	Traumatic musculoskeletal injuries that result in bone defects or fractures	191:265	Traumatic musculoskeletal injuries that result in bone defects or fractures often affect both bone and the surrounding soft tissue.					
30685477	5	70	from	regeneration	1041:1052	arg1	injuries					1067:1074	composite injuries	1057:1074	composite injuries	1057:1074	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	9	71	theme	bone	1746:1749	arg1	healing					1751:1757	bone healing	1746:1757	bone healing	1746:1757	Contrary to our hypothesis, BMP + MVF decreased the number of blood vessels relative to BMP alone and had no effect on bone healing.					
30685477	4	72	theme	bone	709:712	arg1	healing					714:720	impaired bone healing	700:720	impaired bone healing	700:720	Our lab has previously established a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing; however, the vascularization response in this model had not yet been investigated.					
30685477	0	73	theme	injury	147:152	arg1	model					154:158	a composite bone-muscle injury model	123:158	a composite bone-muscle injury model with impaired vascularization	123:188	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	3	74	theme	healing	581:587	arg1	process					589:595	the bone healing process	572:595	the bone healing process	572:595	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	6	75	theme	microvascular	1192:1204	arg1	MVF					1217:1219	MVF	1217:1219	MVF	1217:1219	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	75	theme	microvascular	1192:1204	arg1	fragments					1206:1214	microvascular fragments	1192:1214	microvascular fragments (MVF)	1192:1220	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	4	76	theme	composite	657:665	arg1	injury					679:684	composite bone-muscle injury	657:684	composite bone-muscle injury	657:684	Our lab has previously established a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing; however, the vascularization response in this model had not yet been investigated.					
30685477	10	77	theme	challenging	1869:1879	arg1	model					1898:1902	the challenging composite defect model	1865:1902	the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system	1865:1993	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	10	77	theme	challenging	1869:1879	arg1	comparable					1912:1921	comparable	1912:1921	comparable	1912:1921	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	4	78	theme	injury	679:684	arg1	model					648:652	a pre-clinical model	633:652	a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing	633:720	Our lab has previously established a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing; however, the vascularization response in this model had not yet been investigated.					
30685477	5	79	theme	therapeutic	930:940	arg1	potential					942:950	the therapeutic potential	926:950	the therapeutic potential of combined vascularization and osteoinduction	926:997	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	6	80	theme	vasculature	1266:1276	arg1	factor					1352:1357	a potent osteoinductive growth factor	1321:1357	a potent osteoinductive growth factor	1321:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	80	theme	vasculature	1266:1276	arg1	BMP-2					1313:1317	BMP-2	1313:1317	BMP-2	1313:1317	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	80	theme	vasculature	1266:1276	arg1	which					1223:1227	which	1223:1227	which	1223:1227	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	80	theme	vasculature	1266:1276	arg1	segments					1247:1254	multicellular segments	1233:1254	multicellular segments of mature vasculature	1233:1276	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	80	theme	vasculature	1266:1276	arg1	protein-2					1302:1310	bone morphogenetic protein-2	1283:1310	bone morphogenetic protein-2 (BMP-2)	1283:1318	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	4	81	theme	vascularization	736:750	arg1	response					752:759	the vascularization response	732:759	the vascularization response in this model	732:773	Our lab has previously established a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing; however, the vascularization response in this model had not yet been investigated.					
30685477	6	82	theme	growth	1345:1350	arg1	factor					1352:1357	a potent osteoinductive growth factor	1321:1357	a potent osteoinductive growth factor	1321:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	82	theme	growth	1345:1350	arg1	protein-2					1302:1310	bone morphogenetic protein-2	1283:1310	bone morphogenetic protein-2 (BMP-2)	1283:1318	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	5	83	theme	impaired	1032:1039	arg1	regeneration					1041:1052	the impaired regeneration	1028:1052	the impaired regeneration in composite injuries	1028:1074	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	10	84	theme	capable	1832:1838	arg1	collagen + DCN					1769:1782	collagen + DCN	1769:1782	collagen + DCN	1769:1782	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	10	84	theme	capable	1832:1838	arg1	vehicle					1824:1830	a BMP-2 delivery vehicle	1807:1830	a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system	1807:1993	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	0	85	theme	protein-2	82:90	arg1	co-delivery					48:58	the co-delivery	44:58	the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization	44:188	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	10	86	theme	defect	1891:1896	arg1	model					1898:1902	the challenging composite defect model	1865:1902	the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system	1865:1993	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	10	86	theme	defect	1891:1896	arg1	comparable					1912:1921	comparable	1912:1921	comparable	1912:1921	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	12	87	theme	early	2170:2174	arg1	response					2194:2201	the early revascularization response	2166:2201	the early revascularization response	2166:2201	For the first time, this work shows that the early revascularization response is also significantly, albeit modestly, impaired.					
30685477	11	88	theme	bone	2111:2114	arg1	healing					2116:2122	impaired bone healing	2102:2122	impaired bone healing	2102:2122	STATEMENT OF SIGNIFICANCE: We have previously established a model of musculoskeletal trauma that exhibits impaired bone healing.					
30685477	6	89	theme	multicellular	1233:1245	arg1	which					1223:1227	which	1223:1227	which	1223:1227	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	89	theme	multicellular	1233:1245	arg1	segments					1247:1254	multicellular segments	1233:1254	multicellular segments of mature vasculature	1233:1276	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	0	90	theme	microvascular	96:108	arg1	fragments					110:118	microvascular fragments	96:118	microvascular fragments	96:118	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	13	91	theme	growth	2423:2428	arg1	factor					2430:2435	an osteoinductive growth factor	2405:2435	an osteoinductive growth factor	2405:2435	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	13	91	theme	growth	2423:2428	arg1	BMP-2					2438:2442	BMP-2	2438:2442	BMP-2	2438:2442	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	8	92	theme	subsequent	1582:1591	arg1	regeneration					1613:1624	subsequent BMP-2-mediated bone regeneration	1582:1624	subsequent BMP-2-mediated bone regeneration	1582:1624	We further hypothesized that MVF would increase both early vascularization and subsequent BMP-2-mediated bone regeneration.					
30685477	0	93	theme	composite	125:133	arg1	model					154:158	a composite bone-muscle injury model	123:158	a composite bone-muscle injury model with impaired vascularization	123:188	Decorin-supplemented collagen hydrogels for the co-delivery of bone morphogenetic protein-2 and microvascular fragments to a composite bone-muscle injury model with impaired vascularization.					
30685477	1	94	theme	bone	241:244	arg1	defects					246:252	bone defects	241:252	bone defects	241:252	Traumatic musculoskeletal injuries that result in bone defects or fractures often affect both bone and the surrounding soft tissue.					
30685477	8	95	theme	bone	1608:1611	arg1	regeneration					1613:1624	subsequent BMP-2-mediated bone regeneration	1582:1624	subsequent BMP-2-mediated bone regeneration	1582:1624	We further hypothesized that MVF would increase both early vascularization and subsequent BMP-2-mediated bone regeneration.					
30685477	3	96	theme	injury	486:491	arg1	severity					493:500	injury severity	486:500	injury severity	486:500	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	5	97	theme	vascularization	964:978	arg1	potential					942:950	the therapeutic potential	926:950	the therapeutic potential of combined vascularization and osteoinduction	926:997	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	3	98	theme	Vascular	436:443	arg1	indicator					473:481	a key clinical indicator	458:481	a key clinical indicator of injury severity	458:500	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	98	theme	Vascular	436:443	arg1	integrity					445:453	Vascular integrity	436:453	Vascular integrity	436:453	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	2	99	theme	long-term	414:422	arg1	disability					424:433	long-term disability	414:433	long-term disability	414:433	Clinically, these types of multi-tissue injuries have increased rates of complications and long-term disability.					
30685477	5	100	theme	bone	845:848	arg1	defect					850:855	a bone defect	843:855	a bone defect	843:855	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	13	101	theme	first	2311:2315	arg1	time					2317:2320	the first time	2307:2320	the first time	2307:2320	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	5	102	theme	osteoinduction	984:997	arg1	potential					942:950	the therapeutic potential	926:950	the therapeutic potential of combined vascularization and osteoinduction	926:997	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	6	103	theme	-supplemented	1092:1104	arg1	hydrogel					1115:1122	-supplemented collagen hydrogel	1092:1122	-supplemented collagen hydrogel	1092:1122	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	103	theme	-supplemented	1092:1104	arg1	vehicle					1164:1170	a biomaterial delivery vehicle	1141:1170	a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor	1141:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	103	theme	-supplemented	1092:1104	arg1	decorin					1079:1085	A decorin	1077:1085	A decorin	1077:1085	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	15	104	theme	vascular	2772:2779	arg1	networks					2781:2788	pre-formed vascular networks	2761:2788	pre-formed vascular networks	2761:2788	Based on its support of robust angiogenesis in vitro, collagen + DCN may be extended for future use with other vascular therapeutics such as pre-formed vascular networks.					
30685477	14	105	theme	composite	2596:2604	arg1	model					2613:2617	the challenging composite defect model	2580:2617	the challenging composite defect model	2580:2617	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	3	106	theme	key	460:462	arg1	indicator					473:481	a key clinical indicator	458:481	a key clinical indicator of injury severity	458:500	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	106	theme	key	460:462	arg1	integrity					445:453	Vascular integrity	436:453	Vascular integrity	436:453	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	5	107	theme	early	816:820	arg1	revascularization					822:838	the early revascularization	812:838	the early revascularization of a bone defect following composite injury	812:882	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	5	107	theme	early	816:820	arg1	impaired					899:906	impaired	899:906	impaired	899:906	Here, the early revascularization of a bone defect following composite injury is shown to be impaired, and subsequently the therapeutic potential of combined vascularization and osteoinduction was investigated to overcome the impaired regeneration in composite injuries.					
30685477	2	108	theme	complications	396:408	arg1	rates					387:391	increased rates	377:391	increased rates of complications and long-term disability	377:433	Clinically, these types of multi-tissue injuries have increased rates of complications and long-term disability.					
30685477	10	109	theme	well-established	1947:1962	arg1	system					1988:1993	a well-established alginate-based delivery system	1945:1993	a well-established alginate-based delivery system	1945:1993	However, collagen + DCN was demonstrated to be a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model that is comparable to that achieved with a well-established alginate-based delivery system.					
30685477	3	110	theme	site	539:542	arg1	step					564:567	a critical early step	547:567	a critical early step of the bone healing process	547:595	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	110	theme	site	539:542	arg1	revascularization					507:523	revascularization	507:523	revascularization of the injury site	507:542	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	9	111	theme	blood	1689:1693	arg1	vessels					1695:1701	blood vessels	1689:1701	blood vessels	1689:1701	Contrary to our hypothesis, BMP + MVF decreased the number of blood vessels relative to BMP alone and had no effect on bone healing.					
30685477	4	112	theme	pre-clinical	635:646	arg1	model					648:652	a pre-clinical model	633:652	a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing	633:720	Our lab has previously established a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing; however, the vascularization response in this model had not yet been investigated.					
30685477	14	113	theme	vascular	2471:2478	arg1	volume					2480:2485	vascular volume	2471:2485	vascular volume	2471:2485	While MVF did not improve vascular volume or bone healing, collagen + DCN is a BMP-2 delivery vehicle capable of achieving bridging in the challenging composite defect model.					
30685477	2	114	theme	multi-tissue	350:361	arg1	injuries					363:370	multi-tissue injuries	350:370	multi-tissue injuries	350:370	Clinically, these types of multi-tissue injuries have increased rates of complications and long-term disability.					
30685477	4	115	from	response	752:759	arg1	model					769:773	this model	764:773	this model	764:773	Our lab has previously established a pre-clinical model of composite bone-muscle injury that exhibits impaired bone healing; however, the vascularization response in this model had not yet been investigated.					
30685477	12	116	theme	revascularization	2176:2192	arg1	response					2194:2201	the early revascularization response	2166:2201	the early revascularization response	2166:2201	For the first time, this work shows that the early revascularization response is also significantly, albeit modestly, impaired.					
30685477	6	117	theme	delivery	1155:1162	arg1	hydrogel					1115:1122	-supplemented collagen hydrogel	1092:1122	-supplemented collagen hydrogel	1092:1122	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	117	theme	delivery	1155:1162	arg1	vehicle					1164:1170	a biomaterial delivery vehicle	1141:1170	a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor	1141:1357	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	6	117	theme	delivery	1155:1162	arg1	decorin					1079:1085	A decorin	1077:1085	A decorin	1077:1085	A decorin (DCN)-supplemented collagen hydrogel was developed as a biomaterial delivery vehicle for the co-delivery microvascular fragments (MVF), which are multicellular segments of mature vasculature, and bone morphogenetic protein-2 (BMP-2), a potent osteoinductive growth factor.					
30685477	3	118	theme	early	558:562	arg1	step					564:567	a critical early step	547:567	a critical early step of the bone healing process	547:595	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	3	118	theme	early	558:562	arg1	revascularization					507:523	revascularization	507:523	revascularization of the injury site	507:542	Vascular integrity is a key clinical indicator of injury severity, and revascularization of the injury site is a critical early step of the bone healing process.					
30685477	13	119	theme	collagen	2276:2283	arg1	hydrogel					2285:2292	A decorin-supplemented collagen hydrogel	2253:2292	A decorin-supplemented collagen hydrogel	2253:2292	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
30685477	13	119	theme	collagen	2276:2283	arg1	vehicle					2344:2350	a delivery vehicle	2333:2350	a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2	2333:2442	A decorin-supplemented collagen hydrogel was used for the first time in vivo as a delivery vehicle for both a cell-based vascular therapeutic, MVF, and an osteoinductive growth factor, BMP-2.					
31755264	4	0	theme	food	469:472	arg1	substrates					474:483	food substrates	469:483	food substrates	469:483	However, the composition of viral ligands from food substrates remains unknown.					
31755264	9	1	with	interaction	1033:1043	arg1	contaminated					1067:1078	contaminated	1067:1078	contaminated	1067:1078	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
31755264	7	2	theme	ELISA	847:851	arg1	results					853:859	our ELISA results	843:859	our ELISA results using a panel of monoclonal antibodies against HBGAs	843:912	The composition was consistent with our ELISA results using a panel of monoclonal antibodies against HBGAs.					
31755264	9	3	theme	disease	1143:1149	arg1	outbreaks					1151:1159	disease outbreaks	1143:1159	disease outbreaks	1143:1159	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
31755264	9	4	from	outbreaks	1151:1159	arg1	control					1115:1121	the control	1111:1121	the control of and reduction in disease outbreaks	1111:1159	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
31755264	0	5	theme	Human	102:106	arg1	Attachment					118:127	Human Norovirus Attachment	102:127	Human Norovirus Attachment	102:127	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	0	6	from	Characterization	0:15	arg1	Lettuce					74:80	Romaine Lettuce	66:80	Romaine Lettuce	66:80	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	6	7	theme	H	785:785	arg1	HBGAs					800:804	type A, H, and Lewis a HBGAs	777:804	type A, H, and Lewis a HBGAs	777:804	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	2	8	theme	HuNoV	299:303	arg1	binding					314:320	HuNoV specific binding	299:320	HuNoV specific binding	299:320	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	9	9	theme	interaction	1033:1043	arg1	understanding					1012:1024	Better understanding	1005:1024	Better understanding of the interaction of HuNoVs with easily contaminated produce	1005:1086	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
31755264	9	10	from	control	1115:1121	arg1	outbreaks					1151:1159	disease outbreaks	1143:1159	disease outbreaks	1143:1159	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
31755264	8	11	theme	interaction	947:957	arg1	mechanism					959:967	a possible interaction mechanism	936:967	a possible interaction mechanism between HuNoVs and romaine lettuce	936:1002	Our results revealed a possible interaction mechanism between HuNoVs and romaine lettuce.					
31755264	9	12	theme	HuNoVs	1048:1053	arg1	interaction					1033:1043	the interaction	1029:1043	the interaction of HuNoVs with easily contaminated produce	1029:1086	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
31755264	0	13	from	Substance	53:61	arg1	Lettuce					74:80	Romaine Lettuce	66:80	Romaine Lettuce	66:80	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	5	14	theme	surface	615:621	arg1	system					631:636	a bacterial surface display system	603:636	a bacterial surface display system	603:636	In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system.					
31755264	5	15	theme	bacterial	605:613	arg1	system					631:636	a bacterial surface display system	603:636	a bacterial surface display system	603:636	In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system.					
31755264	6	16	theme	electrospray	645:656	arg1	spectrometry					674:685	electrospray ionization mass spectrometry	645:685	electrospray ionization mass spectrometry	645:685	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	9	17	from	reduction	1130:1138	arg1	outbreaks					1151:1159	disease outbreaks	1143:1159	disease outbreaks	1143:1159	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
31755264	0	18	theme	Norovirus	108:116	arg1	Attachment					118:127	Human Norovirus Attachment	102:127	Human Norovirus Attachment	102:127	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	5	19	theme	display	623:629	arg1	system					631:636	a bacterial surface display system	603:636	a bacterial surface display system	603:636	In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system.					
31755264	6	20	theme	type	777:780	arg1	A					782:782	type A	777:782	type A	777:782	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	2	21	theme	accepted	276:283	arg1	antigens					248:255	Histo-blood group antigens	230:255	Histo-blood group antigens (HBGAs)	230:263	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	2	21	theme	accepted	276:283	arg1	receptors					285:293	widely accepted receptors	269:293	widely accepted receptors for HuNoV specific binding	269:320	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	1	22	theme	main	171:174	arg1	pathogens					176:184	the main pathogens	167:184	the main pathogens causing acute nonbacterial gastroenteritis	167:227	Human noroviruses (HuNoVs) are among the main pathogens causing acute nonbacterial gastroenteritis.					
31755264	7	23	theme	antibodies	889:898	arg1	panel					869:873	a panel	867:873	a panel of monoclonal antibodies against HBGAs	867:912	The composition was consistent with our ELISA results using a panel of monoclonal antibodies against HBGAs.					
31755264	0	24	theme	Histo-Blood	22:32	arg1	Substance					53:61	a Histo-Blood Group Antigen-like Substance	20:61	a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That	20:85	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	5	25	theme	romaine	576:582	arg1	extract					592:598	romaine lettuce extract	576:598	romaine lettuce extract	576:598	In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system.					
31755264	4	26	from	substrates	474:483	arg1	composition					435:445	the composition	431:445	the composition of viral ligands from food substrates	431:483	However, the composition of viral ligands from food substrates remains unknown.					
31755264	0	27	theme	Substance	53:61	arg1	Characterization					0:15	Characterization	0:15	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That	0:85	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	3	28	theme	critical	382:389	arg1	substances					333:342	HBGA-like substances	323:342	HBGA-like substances in produce	323:353	HBGA-like substances in produce are also considered as the critical ligands for capture of HuNoVs.					
31755264	3	28	theme	critical	382:389	arg1	ligands					391:397	the critical ligands	378:397	the critical ligands for capture of HuNoVs	378:419	HBGA-like substances in produce are also considered as the critical ligands for capture of HuNoVs.					
31755264	5	29	theme	lettuce	584:590	arg1	extract					592:598	romaine lettuce extract	576:598	romaine lettuce extract	576:598	In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system.					
31755264	6	30	theme	tandem	691:696	arg1	spectrometry					703:714	tandem mass spectrometry	691:714	tandem mass spectrometry	691:714	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	1	31	theme	acute	194:198	arg1	gastroenteritis					213:227	acute nonbacterial gastroenteritis	194:227	acute nonbacterial gastroenteritis	194:227	Human noroviruses (HuNoVs) are among the main pathogens causing acute nonbacterial gastroenteritis.					
31755264	6	32	theme	HBGAs	800:804	arg1	H2N2F2					735:740	H2N2F2	735:740	H2N2F2	735:740	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	6	32	theme	HBGAs	800:804	arg1	chimera					766:772	a chimera	764:772	a chimera of type A, H, and Lewis a HBGAs	764:804	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	4	33	theme	ligands	456:462	arg1	composition					435:445	the composition	431:445	the composition of viral ligands from food substrates	431:483	However, the composition of viral ligands from food substrates remains unknown.					
31755264	2	34	theme	specific	305:312	arg1	binding					314:320	HuNoV specific binding	299:320	HuNoV specific binding	299:320	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	1	35	theme	nonbacterial	200:211	arg1	gastroenteritis					213:227	acute nonbacterial gastroenteritis	194:227	acute nonbacterial gastroenteritis	194:227	Human noroviruses (HuNoVs) are among the main pathogens causing acute nonbacterial gastroenteritis.					
31755264	0	36	theme	Antigen-like	40:51	arg1	Substance					53:61	a Histo-Blood Group Antigen-like Substance	20:61	a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That	20:85	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	8	37	dep	HuNoVs	977:982	arg1	lettuce					996:1002	lettuce	996:1002	lettuce	996:1002	Our results revealed a possible interaction mechanism between HuNoVs and romaine lettuce.					
31755264	4	38	theme	viral	450:454	arg1	ligands					456:462	viral ligands	450:462	viral ligands	450:462	However, the composition of viral ligands from food substrates remains unknown.					
31755264	0	39	theme	Group	34:38	arg1	Substance					53:61	a Histo-Blood Group Antigen-like Substance	20:61	a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That	20:85	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	5	40	attach	isolated	562:569	arg2	H2N2F2					537:542	H2N2F2	537:542	H2N2F2	537:542	In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system.					
31755264	5	40	attach	isolated	562:569	arg1	extract					592:598	romaine lettuce extract	576:598	romaine lettuce extract	576:598	In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system.					
31755264	5	40	attach	isolated	562:569	arg2	oligosaccharide					520:534	an oligosaccharide	517:534	an oligosaccharide (H2N2F2)	517:543	In this study, an oligosaccharide (H2N2F2) was captured and isolated from romaine lettuce extract by a bacterial surface display system.					
31755264	0	41	from	Lettuce	74:80	arg1	Characterization					0:15	Characterization	0:15	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That	0:85	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	0	42	dep	Substance	53:61	arg1	That					82:85	That	82:85	That	82:85	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	9	43	dep	contaminated	1067:1078	arg1	produce					1080:1086	produce	1080:1086	produce	1080:1086	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
31755264	2	44	theme	group	242:246	arg1	antigens					248:255	Histo-blood group antigens	230:255	Histo-blood group antigens (HBGAs)	230:263	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	2	44	theme	group	242:246	arg1	receptors					285:293	widely accepted receptors	269:293	widely accepted receptors for HuNoV specific binding	269:320	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	2	44	theme	group	242:246	arg1	HBGAs					258:262	HBGAs	258:262	HBGAs	258:262	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	3	45	theme	HuNoVs	414:419	arg1	capture					403:409	capture	403:409	capture of HuNoVs	403:419	HBGA-like substances in produce are also considered as the critical ligands for capture of HuNoVs.					
31755264	3	46	theme	HBGA-like	323:331	arg1	substances					333:342	HBGA-like substances	323:342	HBGA-like substances in produce	323:353	HBGA-like substances in produce are also considered as the critical ligands for capture of HuNoVs.					
31755264	3	46	theme	HBGA-like	323:331	arg1	ligands					391:397	the critical ligands	378:397	the critical ligands for capture of HuNoVs	378:419	HBGA-like substances in produce are also considered as the critical ligands for capture of HuNoVs.					
31755264	3	47	from	substances	333:342	arg1	produce					347:353	produce	347:353	produce	347:353	HBGA-like substances in produce are also considered as the critical ligands for capture of HuNoVs.					
31755264	2	48	theme	Histo-blood	230:240	arg1	antigens					248:255	Histo-blood group antigens	230:255	Histo-blood group antigens (HBGAs)	230:263	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	2	48	theme	Histo-blood	230:240	arg1	receptors					285:293	widely accepted receptors	269:293	widely accepted receptors for HuNoV specific binding	269:320	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	2	48	theme	Histo-blood	230:240	arg1	HBGAs					258:262	HBGAs	258:262	HBGAs	258:262	Histo-blood group antigens (HBGAs) are widely accepted receptors for HuNoV specific binding.					
31755264	1	49	theme	Human	130:134	arg1	noroviruses					136:146	Human noroviruses	130:146	Human noroviruses (HuNoVs)	130:155	Human noroviruses (HuNoVs) are among the main pathogens causing acute nonbacterial gastroenteritis.					
31755264	1	49	theme	Human	130:134	arg1	HuNoVs					149:154	HuNoVs	149:154	HuNoVs	149:154	Human noroviruses (HuNoVs) are among the main pathogens causing acute nonbacterial gastroenteritis.					
31755264	6	50	theme	mass	669:672	arg1	spectrometry					674:685	electrospray ionization mass spectrometry	645:685	electrospray ionization mass spectrometry	645:685	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	7	51	theme	monoclonal	878:887	arg1	antibodies					889:898	monoclonal antibodies	878:898	monoclonal antibodies against HBGAs	878:912	The composition was consistent with our ELISA results using a panel of monoclonal antibodies against HBGAs.					
31755264	6	52	theme	a	798:798	arg1	HBGAs					800:804	type A, H, and Lewis a HBGAs	777:804	type A, H, and Lewis a HBGAs	777:804	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	6	53	theme	mass	698:701	arg1	spectrometry					703:714	tandem mass spectrometry	691:714	tandem mass spectrometry	691:714	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	8	54	theme	possible	938:945	arg1	mechanism					959:967	a possible interaction mechanism	936:967	a possible interaction mechanism between HuNoVs and romaine lettuce	936:1002	Our results revealed a possible interaction mechanism between HuNoVs and romaine lettuce.					
31755264	6	55	theme	ionization	658:667	arg1	spectrometry					674:685	electrospray ionization mass spectrometry	645:685	electrospray ionization mass spectrometry	645:685	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	6	56	theme	Lewis	792:796	arg1	a					798:798	Lewis a	792:798	Lewis a	792:798	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	6	57	theme	A	782:782	arg1	HBGAs					800:804	type A, H, and Lewis a HBGAs	777:804	type A, H, and Lewis a HBGAs	777:804	Using electrospray ionization mass spectrometry and tandem mass spectrometry, it was shown that H2N2F2 was most likely to be a chimera of type A, H, and Lewis a HBGAs.					
31755264	9	58	theme	Better	1005:1010	arg1	understanding					1012:1024	Better understanding	1005:1024	Better understanding of the interaction of HuNoVs with easily contaminated produce	1005:1086	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
31755264	0	59	theme	Romaine	66:72	arg1	Lettuce					74:80	Romaine Lettuce	66:80	Romaine Lettuce	66:80	Characterization of a Histo-Blood Group Antigen-like Substance in Romaine Lettuce That Contributes to Human Norovirus Attachment.					
31755264	7	60	with	consistent	827:836	arg1	results					853:859	our ELISA results	843:859	our ELISA results using a panel of monoclonal antibodies against HBGAs	843:912	The composition was consistent with our ELISA results using a panel of monoclonal antibodies against HBGAs.					
31755264	9	61	theme	reduction	1130:1138	arg1	control					1115:1121	the control	1111:1121	the control of and reduction in disease outbreaks	1111:1159	Better understanding of the interaction of HuNoVs with easily contaminated produce will ultimately aid in the control of and reduction in disease outbreaks.					
30033277	0	0	theme	host-guest	101:110	arg1	interactions					112:123	the host-guest interactions	97:123	the host-guest interactions	97:123	Fabrication and characterization of hyperbranched polyglycerol modified carbon nanotubes through the host-guest interactions.					
30033277	9	1	theme	enormous	1398:1405	arg1	potential					1407:1415	enormous potential	1398:1415	enormous potential	1398:1415	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	10	2	theme	composites	1521:1530	arg1	preparation					1493:1503	the preparation	1489:1503	the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry	1489:1595	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	7	3	theme	reaction	1066:1073	arg1	time					1075:1078	fast reaction time	1061:1078	fast reaction time (30 min)	1061:1087	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	7	3	theme	reaction	1066:1073	arg1	30 min					1081:1086	30 min	1081:1086	30 min	1081:1086	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	4	4	theme	water	735:739	arg1	dispersibility					741:754	excellent water dispersibility	725:754	excellent water dispersibility	725:754	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	1	5	theme	cell	280:283	arg1	capability					306:315	their excellent cell membrane penetration capability	264:315	their excellent cell membrane penetration capability	264:315	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	10	6	theme	biomedical	1629:1638	arg1	applications					1640:1651	biomedical applications	1629:1651	biomedical applications	1629:1651	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	9	7	theme	CNT-β-CD-HPG	1326:1337	arg1	composites					1339:1348	CNT-β-CD-HPG composites	1326:1348	CNT-β-CD-HPG composites	1326:1348	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	10	8	theme	CNT-β-CD-HPG	1508:1519	arg1	composites					1521:1530	CNT-β-CD-HPG composites	1508:1530	CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry	1508:1595	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	2	9	theme	aqueous	392:398	arg1	solution					400:407	aqueous solution	392:407	aqueous solution	392:407	Nevertheless, the poor dispersibility in aqueous solution still perplexes the biomedical applications of CNTs.					
30033277	8	10	theme	characterization	1268:1283	arg1	techniques					1285:1294	characterization techniques	1268:1294	characterization techniques	1268:1294	The successful preparation of CNT-β-CD-HPG composites could be confirmed via a series of characterization techniques.					
30033277	1	11	theme	membrane	285:292	arg1	capability					306:315	their excellent cell membrane penetration capability	264:315	their excellent cell membrane penetration capability	264:315	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	8	12	theme	successful	1183:1192	arg1	preparation					1194:1204	The successful preparation	1179:1204	The successful preparation of CNT-β-CD-HPG composites	1179:1231	The successful preparation of CNT-β-CD-HPG composites could be confirmed via a series of characterization techniques.					
30033277	6	13	theme	hydroxyl	938:945	arg1	group					947:951	the hydroxyl group	934:951	the hydroxyl group of radiant CNTs	934:967	On the other hand, adamantane chloride was first reacted with the hydroxyl group of radiant CNTs through esterification to obtain CNT-Ad.					
30033277	6	13	theme	hydroxyl	938:945	arg1	CNTs					964:967	radiant CNTs	956:967	radiant CNTs	956:967	On the other hand, adamantane chloride was first reacted with the hydroxyl group of radiant CNTs through esterification to obtain CNT-Ad.					
30033277	7	14	theme	inclusion	1121:1129	arg1	complex					1131:1137	an exact 1:1 inclusion complex	1108:1137	an exact 1:1 inclusion complex with CNT-Ad	1108:1149	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	1	15	theme	penetration	294:304	arg1	capability					306:315	their excellent cell membrane penetration capability	264:315	their excellent cell membrane penetration capability	264:315	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	4	16	theme	efficient	657:665	arg1	strategy					678:685	an efficient and facile strategy	654:685	an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility	654:754	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	10	17	from	prospects	1616:1624	arg1	applications					1640:1651	biomedical applications	1629:1651	biomedical applications	1629:1651	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	4	18	theme	excellent	725:733	arg1	dispersibility					741:754	excellent water dispersibility	725:754	excellent water dispersibility	725:754	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	7	19	theme	exact	1111:1115	arg1	complex					1131:1137	an exact 1:1 inclusion complex	1108:1137	an exact 1:1 inclusion complex with CNT-Ad	1108:1149	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	2	20	from	dispersibility	374:387	arg1	solution					400:407	aqueous solution	392:407	aqueous solution	392:407	Nevertheless, the poor dispersibility in aqueous solution still perplexes the biomedical applications of CNTs.					
30033277	2	21	theme	biomedical	429:438	arg1	applications					440:451	the biomedical applications	425:451	the biomedical applications of CNTs	425:459	Nevertheless, the poor dispersibility in aqueous solution still perplexes the biomedical applications of CNTs.					
30033277	4	22	theme	surface	691:697	arg1	modification					699:710	surface modification	691:710	surface modification of CNTs with excellent water dispersibility	691:754	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	9	23	theme	remarkable	1362:1371	arg1	dispersibility					1379:1392	the remarkable water dispersibility	1358:1392	the remarkable water dispersibility	1358:1392	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	7	24	theme	1:1	1117:1119	arg1	complex					1131:1137	an exact 1:1 inclusion complex	1108:1137	an exact 1:1 inclusion complex with CNT-Ad	1108:1149	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	9	25	contain	possess	1350:1356	arg2	dispersibility					1379:1392	the remarkable water dispersibility	1358:1392	the remarkable water dispersibility	1358:1392	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	9	25	contain	possess	1350:1356	arg1	composites					1339:1348	CNT-β-CD-HPG composites	1326:1348	CNT-β-CD-HPG composites	1326:1348	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	9	25	contain	possess	1350:1356	arg2	potential					1407:1415	enormous potential	1398:1415	enormous potential	1398:1415	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	3	26	theme	efficient	572:580	arg1	strategies					582:591	facile and efficient strategies	561:591	facile and efficient strategies	561:591	Although, there are many researched about that modify hydrophilic polymers to the surface of CNTs, facile and efficient strategies are still highly desirable to be developed.					
30033277	10	27	with	composites	1521:1530	arg1	dispersibility					1553:1566	excellent water dispersibility	1537:1566	excellent water dispersibility via supramolecular chemistry	1537:1595	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	8	28	theme	composites	1222:1231	arg1	preparation					1194:1204	The successful preparation	1179:1204	The successful preparation of CNT-β-CD-HPG composites	1179:1231	The successful preparation of CNT-β-CD-HPG composites could be confirmed via a series of characterization techniques.					
30033277	9	29	theme	water	1373:1377	arg1	dispersibility					1379:1392	the remarkable water dispersibility	1358:1392	the remarkable water dispersibility	1358:1392	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	10	30	theme	excellent	1537:1545	arg1	dispersibility					1553:1566	excellent water dispersibility	1537:1566	excellent water dispersibility via supramolecular chemistry	1537:1595	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	1	31	theme	extensive	197:205	arg1	attention					216:224	extensive research attention	197:224	extensive research attention	197:224	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	5	32	theme	anionic	848:854	arg1	polymerization					856:869	anionic polymerization	848:869	anionic polymerization	848:869	On the one hand, we synthesize the β-CD-HPG via anionic polymerization.					
30033277	4	33	dep	this	640:643	arg1	produce					645:651	produce	645:651	produce	645:651	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	4	34	theme	supramolecular	774:787	arg1	chemistry					789:797	supramolecular chemistry	774:797	supramolecular chemistry	774:797	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	4	35	mod	modification	699:710	arg1	CNTs					715:718	CNTs	715:718	CNTs	715:718	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	4	35	mod	modification	699:710	arg3	surface					691:697	surface modification	691:710	surface modification of CNTs with excellent water dispersibility	691:754	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	1	36	theme	research	207:214	arg1	attention					216:224	extensive research attention	197:224	extensive research attention	197:224	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	1	37	theme	large	321:325	arg1	areas					344:348	large specific surface areas	321:348	large specific surface areas	321:348	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	10	38	theme	facile	1469:1474	arg1	strategy					1476:1483	the facile strategy	1465:1483	the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry	1465:1595	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	1	39	theme	Carbon	126:131	arg1	composites					167:176	novel carbon composites	154:176	novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas	154:348	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	1	39	theme	Carbon	126:131	arg1	CNTs					144:147	CNTs	144:147	CNTs	144:147	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	1	39	theme	Carbon	126:131	arg1	nanotubes					133:141	Carbon nanotubes	126:141	Carbon nanotubes (CNTs)	126:148	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	1	40	theme	specific	327:334	arg1	areas					344:348	large specific surface areas	321:348	large specific surface areas	321:348	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	3	41	theme	CNTs	555:558	arg1	surface					544:550	the surface	540:550	the surface of CNTs	540:558	Although, there are many researched about that modify hydrophilic polymers to the surface of CNTs, facile and efficient strategies are still highly desirable to be developed.					
30033277	3	42	theme	hydrophilic	516:526	arg1	polymers					528:535	hydrophilic polymers	516:535	hydrophilic polymers	516:535	Although, there are many researched about that modify hydrophilic polymers to the surface of CNTs, facile and efficient strategies are still highly desirable to be developed.					
30033277	0	43	theme	polyglycerol	50:61	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of hyperbranched polyglycerol modified carbon nanotubes through the host-guest interactions.					
30033277	0	43	theme	polyglycerol	50:61	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of hyperbranched polyglycerol modified carbon nanotubes through the host-guest interactions.					
30033277	1	44	theme	surface	336:342	arg1	areas					344:348	large specific surface areas	321:348	large specific surface areas	321:348	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	10	45	theme	supramolecular	1572:1585	arg1	chemistry					1587:1595	supramolecular chemistry	1572:1595	supramolecular chemistry	1572:1595	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	4	46	with	modification	699:710	arg1	dispersibility					741:754	excellent water dispersibility	725:754	excellent water dispersibility	725:754	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	8	47	theme	techniques	1285:1294	arg1	series					1258:1263	a series	1256:1263	a series of characterization techniques	1256:1294	The successful preparation of CNT-β-CD-HPG composites could be confirmed via a series of characterization techniques.					
30033277	1	48	theme	biomedical	230:239	arg1	applications					241:252	biomedical applications	230:252	biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas	230:348	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	0	49	theme	hyperbranched	36:48	arg1	polyglycerol					50:61	hyperbranched polyglycerol	36:61	hyperbranched polyglycerol	36:61	Fabrication and characterization of hyperbranched polyglycerol modified carbon nanotubes through the host-guest interactions.					
30033277	9	50	theme	delivery	1437:1444	arg1	systems					1446:1452	controlled drug delivery systems	1421:1452	controlled drug delivery systems	1421:1452	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	6	51	theme	adamantane	891:900	arg1	chloride					902:909	adamantane chloride	891:909	adamantane chloride	891:909	On the other hand, adamantane chloride was first reacted with the hydroxyl group of radiant CNTs through esterification to obtain CNT-Ad.					
30033277	2	52	theme	CNTs	456:459	arg1	applications					440:451	the biomedical applications	425:451	the biomedical applications of CNTs	425:459	Nevertheless, the poor dispersibility in aqueous solution still perplexes the biomedical applications of CNTs.					
30033277	0	53	theme	carbon	72:77	arg1	nanotubes					79:87	carbon nanotubes	72:87	carbon nanotubes	72:87	Fabrication and characterization of hyperbranched polyglycerol modified carbon nanotubes through the host-guest interactions.					
30033277	9	54	theme	controlled	1421:1430	arg1	systems					1446:1452	controlled drug delivery systems	1421:1452	controlled drug delivery systems	1421:1452	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	10	55	theme	rosy	1611:1614	arg1	prospects					1616:1624	rosy prospects	1611:1624	rosy prospects in biomedical applications	1611:1651	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	7	56	theme	host-guest	1155:1164	arg1	interaction					1166:1176	host-guest interaction	1155:1176	host-guest interaction	1155:1176	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	9	57	theme	drug	1432:1435	arg1	systems					1446:1452	controlled drug delivery systems	1421:1452	controlled drug delivery systems	1421:1452	Then, we further verify that CNT-β-CD-HPG composites possess the remarkable water dispersibility and enormous potential for controlled drug delivery systems.					
30033277	10	58	theme	water	1547:1551	arg1	dispersibility					1553:1566	excellent water dispersibility	1537:1566	excellent water dispersibility via supramolecular chemistry	1537:1595	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	7	59	theme	mild	1032:1035	arg1	conditions					1046:1055	mild reaction conditions	1032:1055	mild reaction conditions	1032:1055	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	6	60	theme	CNTs	964:967	arg1	group					947:951	the hydroxyl group	934:951	the hydroxyl group of radiant CNTs	934:967	On the other hand, adamantane chloride was first reacted with the hydroxyl group of radiant CNTs through esterification to obtain CNT-Ad.					
30033277	6	60	theme	CNTs	964:967	arg1	CNTs					964:967	radiant CNTs	956:967	radiant CNTs	956:967	On the other hand, adamantane chloride was first reacted with the hydroxyl group of radiant CNTs through esterification to obtain CNT-Ad.					
30033277	7	61	theme	fast	1061:1064	arg1	time					1075:1078	fast reaction time	1061:1078	fast reaction time (30 min)	1061:1087	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	7	61	theme	fast	1061:1064	arg1	30 min					1081:1086	30 min	1081:1086	30 min	1081:1086	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	6	62	theme	other	879:883	arg1	hand					885:888	the other hand	875:888	the other hand	875:888	On the other hand, adamantane chloride was first reacted with the hydroxyl group of radiant CNTs through esterification to obtain CNT-Ad.					
30033277	4	63	theme	CNTs	715:718	arg1	modification					699:710	surface modification	691:710	surface modification of CNTs with excellent water dispersibility	691:754	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	2	64	theme	poor	369:372	arg1	dispersibility					374:387	the poor dispersibility	365:387	the poor dispersibility in aqueous solution	365:407	Nevertheless, the poor dispersibility in aqueous solution still perplexes the biomedical applications of CNTs.					
30033277	1	65	theme	thanks	254:259	arg1	applications					241:252	biomedical applications	230:252	biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas	230:348	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	7	66	theme	reaction	1037:1044	arg1	conditions					1046:1055	mild reaction conditions	1032:1055	mild reaction conditions	1032:1055	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	8	67	theme	CNT-β-CD-HPG	1209:1220	arg1	composites					1222:1231	CNT-β-CD-HPG composites	1209:1231	CNT-β-CD-HPG composites	1209:1231	The successful preparation of CNT-β-CD-HPG composites could be confirmed via a series of characterization techniques.					
30033277	3	68	theme	facile	561:566	arg1	strategies					582:591	facile and efficient strategies	561:591	facile and efficient strategies	561:591	Although, there are many researched about that modify hydrophilic polymers to the surface of CNTs, facile and efficient strategies are still highly desirable to be developed.					
30033277	4	69	theme	facile	671:676	arg1	strategy					678:685	an efficient and facile strategy	654:685	an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility	654:754	In this produce, an efficient and facile strategy for surface modification of CNTs with excellent water dispersibility was developed via supramolecular chemistry.					
30033277	1	70	theme	novel	154:158	arg1	composites					167:176	novel carbon composites	154:176	novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas	154:348	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	1	70	theme	novel	154:158	arg1	nanotubes					133:141	Carbon nanotubes	126:141	Carbon nanotubes (CNTs)	126:148	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	10	71	contain	possess	1603:1609	arg1	strategy					1476:1483	the facile strategy	1465:1483	the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry	1465:1595	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	10	71	contain	possess	1603:1609	arg2	prospects					1616:1624	rosy prospects	1611:1624	rosy prospects in biomedical applications	1611:1651	Therefore the facile strategy for the preparation of CNT-β-CD-HPG composites with excellent water dispersibility via supramolecular chemistry would possess rosy prospects in biomedical applications.					
30033277	6	72	theme	radiant	956:962	arg1	CNTs					964:967	radiant CNTs	956:967	radiant CNTs	956:967	On the other hand, adamantane chloride was first reacted with the hydroxyl group of radiant CNTs through esterification to obtain CNT-Ad.					
30033277	1	73	theme	carbon	160:165	arg1	composites					167:176	novel carbon composites	154:176	novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas	154:348	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	1	73	theme	carbon	160:165	arg1	nanotubes					133:141	Carbon nanotubes	126:141	Carbon nanotubes (CNTs)	126:148	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
30033277	7	74	with	complex	1131:1137	arg1	CNT-Ad					1144:1149	CNT-Ad	1144:1149	CNT-Ad	1144:1149	Finally, it only need mild reaction conditions and fast reaction time (30 min) that β-CD-HPG form an exact 1:1 inclusion complex with CNT-Ad via host-guest interaction.					
30033277	1	75	theme	excellent	270:278	arg1	capability					306:315	their excellent cell membrane penetration capability	264:315	their excellent cell membrane penetration capability	264:315	Carbon nanotubes (CNTs) are novel carbon composites that have received extensive research attention for biomedical applications thanks to their excellent cell membrane penetration capability and large specific surface areas.					
31542584	6	0	theme	-IP	1355:1357	arg1	beads					1359:1363	the Pb(II)-IP beads	1345:1363	the Pb(II)-IP beads	1345:1363	In addition, the studies regarding the regeneration and reuse studies revealed that the Pb(II)-IP beads showed no significant decrease in their adsorption capacities.					
31542584	2	1	with	leaching	473:480	arg1	HNO3					506:509	HNO3	506:509	HNO3	506:509	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	2	1	with	leaching	473:480	arg1	acid					500:503	0.1 M nitric acid	487:503	0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions	487:611	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	1	2	theme	functional	210:219	arg1	monomer					221:227	a functional monomer	208:227	a functional monomer	208:227	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	1	2	theme	functional	210:219	arg1	N'-Methylenebisacrylamide					235:259	N'-Methylenebisacrylamide	235:259	N'-Methylenebisacrylamide (MBA)	235:265	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	1	2	theme	functional	210:219	arg1	chitosan					115:122	A new chitosan	109:122	A new chitosan based imprinted polymer	109:146	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	0	3	theme	aqueous	44:50	arg1	solution					52:59	aqueous solution	44:59	aqueous solution	44:59	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.					
31542584	1	4	from	crosslinker	272:282	arg1	presence					291:298	the presence	287:298	the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions	287:392	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	1	5	theme	imprinted	130:138	arg1	polymer					140:146	imprinted polymer	130:146	imprinted polymer	130:146	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	3	6	theme	Infrared	752:759	arg1	Spectroscopy					761:772	Infrared Spectroscopy	752:772	Infrared Spectroscopy (FT-IR)	752:780	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	3	6	theme	Infrared	752:759	arg1	FT-IR					775:779	FT-IR	775:779	FT-IR	775:779	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	3	7	dep	using	674:678	arg1	Transform					742:750	Transform	742:750	Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA)	742:819	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	0	8	theme	chitosan-lead	64:76	arg1	network					100:106	chitosan-lead ion imprinted polymer network	64:106	chitosan-lead ion imprinted polymer network	64:106	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.					
31542584	5	9	theme	competitive	1010:1020	arg1	studies					1033:1039	The competitive adsorption studies	1006:1039	The competitive adsorption studies	1006:1039	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	3	10	theme	Electron	704:711	arg1	Microscopy					713:722	Field Emission Scanning Electron Microscopy	680:722	Field Emission Scanning Electron Microscopy (FE-SEM)	680:731	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	3	10	theme	Electron	704:711	arg1	FE-SEM					725:730	FE-SEM	725:730	FE-SEM	725:730	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	2	11	theme	polymer	445:451	arg1	particles					460:468	ion imprinted polymer (IIPs) particles	431:468	ion imprinted polymer (IIPs) particles	431:468	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	1	12	theme	Potassium	303:311	arg1	KPS					330:332	Potassium peroxodisulfate (KPS)	303:333	Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions	303:392	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	5	13	theme	adsorption	1093:1102	arg1	capacity					1104:1111	a much higher adsorption capacity	1079:1111	a much higher adsorption capacity for Pb(II)	1079:1122	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	5	14	theme	chemical	1175:1182	arg1	composition					1184:1194	the same chemical composition	1166:1194	the same chemical composition	1166:1194	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	3	15	theme	Thermal	786:792	arg1	TGA					816:818	TGA	816:818	TGA	816:818	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	3	15	theme	Thermal	786:792	arg1	Analysis					806:813	Thermal Gravimetric Analysis	786:813	Thermal Gravimetric Analysis (TGA)	786:819	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	6	16	theme	Pb	1349:1350	arg1	beads					1359:1363	the Pb(II)-IP beads	1345:1363	the Pb(II)-IP beads	1345:1363	In addition, the studies regarding the regeneration and reuse studies revealed that the Pb(II)-IP beads showed no significant decrease in their adsorption capacities.					
31542584	0	17	theme	imprinted	82:90	arg1	network					100:106	chitosan-lead ion imprinted polymer network	64:106	chitosan-lead ion imprinted polymer network	64:106	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.					
31542584	1	18	theme	peroxodisulfate	313:327	arg1	KPS					330:332	Potassium peroxodisulfate (KPS)	303:333	Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions	303:392	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	5	19	contain	has	1075:1077	arg1	-IP					1071:1073	the Pb(II)-IP	1061:1073	the Pb(II)-IP	1061:1073	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	5	19	contain	has	1075:1077	arg2	capacity					1104:1111	a much higher adsorption capacity	1079:1111	a much higher adsorption capacity for Pb(II)	1079:1122	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	1	20	theme	aqueous	376:382	arg1	solutions					384:392	aqueous solutions	376:392	aqueous solutions	376:392	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	3	21	theme	Gravimetric	794:804	arg1	TGA					816:818	TGA	816:818	TGA	816:818	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	3	21	theme	Gravimetric	794:804	arg1	Analysis					806:813	Thermal Gravimetric Analysis	786:813	Thermal Gravimetric Analysis (TGA)	786:819	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	0	22	theme	ion	78:80	arg1	network					100:106	chitosan-lead ion imprinted polymer network	64:106	chitosan-lead ion imprinted polymer network	64:106	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.					
31542584	2	23	theme	template	399:406	arg1	ions					408:411	The template ions	395:411	The template ions	395:411	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	2	24	theme	extraction	583:592	arg1	capability					559:568	the capability	555:568	the capability of selective extraction of the Pb(II) ions	555:611	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	2	25	theme	imprinted	435:443	arg1	particles					460:468	ion imprinted polymer (IIPs) particles	431:468	ion imprinted polymer (IIPs) particles	431:468	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	4	26	theme	Freundlich	902:911	arg1	models					922:927	Freundlich isotherm models	902:927	Freundlich isotherm models	902:927	In addition, the equilibrium adsorption data were examined through Langmuir and Freundlich isotherm models and it was found that adsorption data fit well with the Freundlich isotherm.					
31542584	1	27	theme	KPS	330:332	arg1	presence					291:298	the presence	287:298	the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions	287:392	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	2	28	theme	selective	573:581	arg1	extraction					583:592	selective extraction	573:592	selective extraction of the Pb(II) ions	573:611	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	5	29	theme	same	1170:1173	arg1	composition					1184:1194	the same chemical composition	1166:1194	the same chemical composition	1166:1194	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	0	30	theme	Facile	0:5	arg1	removal					21:27	Facile and efficient removal	0:27	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.	0:107	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.					
31542584	5	31	theme	non-imprinted	1133:1145	arg1	NIP					1156:1158	NIP	1156:1158	NIP	1156:1158	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	5	31	theme	non-imprinted	1133:1145	arg1	polymer					1147:1153	the non-imprinted polymer	1129:1153	the non-imprinted polymer (NIP) with the same chemical composition	1129:1194	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	5	32	theme	targeted	1247:1254	arg1	ion					1256:1258	the targeted ion	1243:1258	the targeted ion	1243:1258	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	6	33	theme	adsorption	1405:1414	arg1	capacities					1416:1425	their adsorption capacities	1399:1425	their adsorption capacities	1399:1425	In addition, the studies regarding the regeneration and reuse studies revealed that the Pb(II)-IP beads showed no significant decrease in their adsorption capacities.					
31542584	0	34	theme	efficient	11:19	arg1	removal					21:27	Facile and efficient removal	0:27	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.	0:107	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.					
31542584	5	35	theme	Pb	1065:1066	arg1	-IP					1071:1073	the Pb(II)-IP	1061:1073	the Pb(II)-IP	1061:1073	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	0	36	theme	polymer	92:98	arg1	network					100:106	chitosan-lead ion imprinted polymer network	64:106	chitosan-lead ion imprinted polymer network	64:106	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.					
31542584	0	37	from	solution	52:59	arg1	removal					21:27	Facile and efficient removal	0:27	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.	0:107	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.					
31542584	4	38	theme	Freundlich	985:994	arg1	isotherm					996:1003	the Freundlich isotherm	981:1003	the Freundlich isotherm	981:1003	In addition, the equilibrium adsorption data were examined through Langmuir and Freundlich isotherm models and it was found that adsorption data fit well with the Freundlich isotherm.					
31542584	4	39	theme	equilibrium	839:849	arg1	data					862:865	the equilibrium adsorption data	835:865	the equilibrium adsorption data	835:865	In addition, the equilibrium adsorption data were examined through Langmuir and Freundlich isotherm models and it was found that adsorption data fit well with the Freundlich isotherm.					
31542584	0	40	theme	Pb	32:33	arg1	removal					21:27	Facile and efficient removal	0:27	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.	0:107	Facile and efficient removal of Pb(II) from aqueous solution by chitosan-lead ion imprinted polymer network.					
31542584	1	41	theme	4-Vinylpyridine	184:198	arg1	copolymerization					164:179	copolymerization	164:179	copolymerization of 4-Vinylpyridine (VP)	164:203	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	6	42	theme	reuse	1317:1321	arg1	studies					1323:1329	the regeneration and reuse studies	1296:1329	the regeneration and reuse studies	1296:1329	In addition, the studies regarding the regeneration and reuse studies revealed that the Pb(II)-IP beads showed no significant decrease in their adsorption capacities.					
31542584	4	43	theme	adsorption	851:860	arg1	data					862:865	the equilibrium adsorption data	835:865	the equilibrium adsorption data	835:865	In addition, the equilibrium adsorption data were examined through Langmuir and Freundlich isotherm models and it was found that adsorption data fit well with the Freundlich isotherm.					
31542584	2	44	theme	IIPs	454:457	arg1	particles					460:468	ion imprinted polymer (IIPs) particles	431:468	ion imprinted polymer (IIPs) particles	431:468	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	2	45	theme	ion	431:433	arg1	particles					460:468	ion imprinted polymer (IIPs) particles	431:468	ion imprinted polymer (IIPs) particles	431:468	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	5	46	theme	adsorption	1022:1031	arg1	studies					1033:1039	The competitive adsorption studies	1006:1039	The competitive adsorption studies	1006:1039	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	2	47	theme	nitric	493:498	arg1	HNO3					506:509	HNO3	506:509	HNO3	506:509	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	2	47	theme	nitric	493:498	arg1	acid					500:503	0.1 M nitric acid	487:503	0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions	487:611	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	5	48	with	polymer	1147:1153	arg1	composition					1184:1194	the same chemical composition	1166:1194	the same chemical composition	1166:1194	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	2	49	theme	Pb	601:602	arg1	ions					608:611	the Pb(II) ions	597:611	the Pb(II) ions	597:611	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	6	50	from	decrease	1387:1394	arg1	capacities					1416:1425	their adsorption capacities	1399:1425	their adsorption capacities	1399:1425	In addition, the studies regarding the regeneration and reuse studies revealed that the Pb(II)-IP beads showed no significant decrease in their adsorption capacities.					
31542584	3	51	theme	bioadsorbent	637:648	arg1	properties					619:628	Some properties	614:628	Some properties of the bioadsorbent	614:648	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	2	52	theme	0.1 M	487:491	arg1	HNO3					506:509	HNO3	506:509	HNO3	506:509	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	2	52	theme	0.1 M	487:491	arg1	acid					500:503	0.1 M nitric acid	487:503	0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions	487:611	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	5	53	contain	has	1213:1215	arg1	it					1210:1211	it	1210:1211	it	1210:1211	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	5	53	contain	has	1213:1215	arg2	selectivity					1227:1237	excellent selectivity	1217:1237	excellent selectivity for the targeted ion	1217:1258	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	4	54	theme	adsorption	951:960	arg1	data					962:965	adsorption data	951:965	adsorption data	951:965	In addition, the equilibrium adsorption data were examined through Langmuir and Freundlich isotherm models and it was found that adsorption data fit well with the Freundlich isotherm.					
31542584	2	55	from	cavities	524:531	arg1	particles					540:548	the particles	536:548	the particles with the capability of selective extraction of the Pb(II) ions	536:611	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	5	56	theme	excellent	1217:1225	arg1	selectivity					1227:1237	excellent selectivity	1217:1237	excellent selectivity for the targeted ion	1217:1258	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	3	57	theme	Emission	686:693	arg1	Microscopy					713:722	Field Emission Scanning Electron Microscopy	680:722	Field Emission Scanning Electron Microscopy (FE-SEM)	680:731	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	3	57	theme	Emission	686:693	arg1	FE-SEM					725:730	FE-SEM	725:730	FE-SEM	725:730	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	6	58	theme	significant	1375:1385	arg1	decrease					1387:1394	no significant decrease	1372:1394	no significant decrease in their adsorption capacities	1372:1425	In addition, the studies regarding the regeneration and reuse studies revealed that the Pb(II)-IP beads showed no significant decrease in their adsorption capacities.					
31542584	2	59	attach	removed	418:424	arg1	particles					460:468	ion imprinted polymer (IIPs) particles	431:468	ion imprinted polymer (IIPs) particles	431:468	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	2	59	attach	removed	418:424	arg2	ions					408:411	The template ions	395:411	The template ions	395:411	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	6	60	theme	regeneration	1300:1311	arg1	studies					1323:1329	the regeneration and reuse studies	1296:1329	the regeneration and reuse studies	1296:1329	In addition, the studies regarding the regeneration and reuse studies revealed that the Pb(II)-IP beads showed no significant decrease in their adsorption capacities.					
31542584	3	61	theme	Scanning	695:702	arg1	Microscopy					713:722	Field Emission Scanning Electron Microscopy	680:722	Field Emission Scanning Electron Microscopy (FE-SEM)	680:731	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	3	61	theme	Scanning	695:702	arg1	FE-SEM					725:730	FE-SEM	725:730	FE-SEM	725:730	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	2	62	with	particles	540:548	arg1	capability					559:568	the capability	555:568	the capability of selective extraction of the Pb(II) ions	555:611	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	2	63	theme	ions	608:611	arg1	extraction					583:592	selective extraction	573:592	selective extraction of the Pb(II) ions	573:611	The template ions were removed from ion imprinted polymer (IIPs) particles by leaching with 0.1 M nitric acid (HNO3) that leaves cavities in the particles with the capability of selective extraction of the Pb(II) ions.					
31542584	1	64	theme	new	111:113	arg1	chitosan					115:122	A new chitosan	109:122	A new chitosan based imprinted polymer	109:146	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	1	64	theme	new	111:113	arg1	N					233:233	N	233:233	N	233:233	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	1	64	theme	new	111:113	arg1	crosslinker					272:282	a crosslinker	270:282	a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions	270:392	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	1	64	theme	new	111:113	arg1	monomer					221:227	a functional monomer	208:227	a functional monomer	208:227	A new chitosan based imprinted polymer was prepared by copolymerization of 4-Vinylpyridine (VP) as a functional monomer and N,N'-Methylenebisacrylamide (MBA) as a crosslinker in the presence of Potassium peroxodisulfate (KPS) as an initiator to eliminate Pb(II) from aqueous solutions.					
31542584	5	65	theme	higher	1086:1091	arg1	capacity					1104:1111	a much higher adsorption capacity	1079:1111	a much higher adsorption capacity for Pb(II)	1079:1122	The competitive adsorption studies clearly showed that the Pb(II)-IP has a much higher adsorption capacity for Pb(II) than the non-imprinted polymer (NIP) with the same chemical composition; furthermore, it has excellent selectivity for the targeted ion.					
31542584	4	66	theme	isotherm	913:920	arg1	models					922:927	Freundlich isotherm models	902:927	Freundlich isotherm models	902:927	In addition, the equilibrium adsorption data were examined through Langmuir and Freundlich isotherm models and it was found that adsorption data fit well with the Freundlich isotherm.					
31542584	3	67	theme	Field	680:684	arg1	Microscopy					713:722	Field Emission Scanning Electron Microscopy	680:722	Field Emission Scanning Electron Microscopy (FE-SEM)	680:731	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
31542584	3	67	theme	Field	680:684	arg1	FE-SEM					725:730	FE-SEM	725:730	FE-SEM	725:730	Some properties of the bioadsorbent were further identified using Field Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Infrared Spectroscopy (FT-IR) and Thermal Gravimetric Analysis (TGA).					
30708008	4	0	theme	dose	617:620	arg1	rate					622:625	dose rate	617:625	dose rate	617:625	The reaction conditions for the adsorption studies were optimized based on initial dye concentration, dose rate, reaction time, pH and temperature.					
30708008	7	1	theme	based	883:887	arg1	composite					889:897	the CPAC based composite	874:897	the CPAC based composite	874:897	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	7	1	theme	based	883:887	arg1	cost					925:928	an effective low cost	908:928	an effective low cost adsorbent for the removal of MG from waste water	908:977	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	1	2	theme	coir	146:149	arg1	CPAC					174:177	CPAC	174:177	CPAC	174:177	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	1	2	theme	coir	146:149	arg1	carbon					166:171	coir pith activated carbon	146:171	coir pith activated carbon (CPAC)	146:178	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	0	3	theme	chitosan	67:74	arg1	composite					76:84	a modified chitosan composite	56:84	a modified chitosan composite	56:84	Removal of malachite green from aqueous solutions using a modified chitosan composite.					
30708008	1	4	theme	pith	151:154	arg1	CPAC					174:177	CPAC	174:177	CPAC	174:177	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	1	4	theme	pith	151:154	arg1	carbon					166:171	coir pith activated carbon	146:171	coir pith activated carbon (CPAC)	146:178	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	2	5	theme	composite	288:296	arg1	characterisation					264:279	The characterisation	260:279	The characterisation of the composite	260:296	The characterisation of the composite was done using SEM, XRD, UV-visible and IR spectroscopy studies.					
30708008	6	6	theme	order	811:815	arg1	kinetics					817:824	pseudo second order kinetics	797:824	pseudo second order kinetics	797:824	The adsorption process was found to follow pseudo second order kinetics.					
30708008	6	7	theme	second	804:809	arg1	kinetics					817:824	pseudo second order kinetics	797:824	pseudo second order kinetics	797:824	The adsorption process was found to follow pseudo second order kinetics.					
30708008	7	8	theme	study	854:858	arg1	results					831:837	The results	827:837	The results of the present study	827:858	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	2	9	theme	SEM	313:315	arg1	studies					354:360	SEM, XRD, UV-visible and IR spectroscopy studies	313:360	SEM, XRD, UV-visible and IR spectroscopy studies	313:360	The characterisation of the composite was done using SEM, XRD, UV-visible and IR spectroscopy studies.					
30708008	1	10	theme	anionic	227:233	arg1	surfactant					235:244	an anionic surfactant	224:244	an anionic surfactant	224:244	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	1	10	theme	anionic	227:233	arg1	SDS					219:221	SDS	219:221	SDS	219:221	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	0	11	from	solutions	40:48	arg1	Removal					0:6	Removal	0:6	Removal of malachite green from aqueous solutions	0:48	Removal of malachite green from aqueous solutions using a modified chitosan composite.					
30708008	4	12	theme	initial	590:596	arg1	concentration					602:614	initial dye concentration	590:614	initial dye concentration	590:614	The reaction conditions for the adsorption studies were optimized based on initial dye concentration, dose rate, reaction time, pH and temperature.					
30708008	1	13	theme	activated	156:164	arg1	CPAC					174:177	CPAC	174:177	CPAC	174:177	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	1	13	theme	activated	156:164	arg1	carbon					166:171	coir pith activated carbon	146:171	coir pith activated carbon (CPAC)	146:178	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	5	14	theme	isotherm	687:694	arg1	models					696:701	Langmuir and Freundlich isotherm models	663:701	Langmuir and Freundlich isotherm models	663:701	Langmuir and Freundlich isotherm models were adopted to study the mechanism of adsorption.					
30708008	3	15	theme	composite	388:396	arg1	effectiveness					367:379	The effectiveness	363:379	The effectiveness of the composite	363:396	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	5	16	theme	Freundlich	676:685	arg1	models					696:701	Langmuir and Freundlich isotherm models	663:701	Langmuir and Freundlich isotherm models	663:701	Langmuir and Freundlich isotherm models were adopted to study the mechanism of adsorption.					
30708008	7	17	theme	waste	967:971	arg1	water					973:977	waste water	967:977	waste water	967:977	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	7	18	theme	present	846:852	arg1	study					854:858	the present study	842:858	the present study	842:858	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	6	19	theme	pseudo	797:802	arg1	kinetics					817:824	pseudo second order kinetics	797:824	pseudo second order kinetics	797:824	The adsorption process was found to follow pseudo second order kinetics.					
30708008	2	20	theme	UV-visible	323:332	arg1	studies					354:360	SEM, XRD, UV-visible and IR spectroscopy studies	313:360	SEM, XRD, UV-visible and IR spectroscopy studies	313:360	The characterisation of the composite was done using SEM, XRD, UV-visible and IR spectroscopy studies.					
30708008	4	21	theme	reaction	628:635	arg1	time					637:640	reaction time	628:640	reaction time	628:640	The reaction conditions for the adsorption studies were optimized based on initial dye concentration, dose rate, reaction time, pH and temperature.					
30708008	0	22	theme	green	21:25	arg1	Removal					0:6	Removal	0:6	Removal of malachite green from aqueous solutions	0:48	Removal of malachite green from aqueous solutions using a modified chitosan composite.					
30708008	7	23	from	water	973:977	arg1	removal					948:954	the removal	944:954	the removal of MG from waste water	944:977	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	7	24	theme	MG	959:960	arg1	removal					948:954	the removal	944:954	the removal of MG from waste water	944:977	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	4	25	theme	adsorption	547:556	arg1	studies					558:564	the adsorption studies	543:564	the adsorption studies	543:564	The reaction conditions for the adsorption studies were optimized based on initial dye concentration, dose rate, reaction time, pH and temperature.					
30708008	0	26	theme	malachite	11:19	arg1	green					21:25	malachite green	11:25	malachite green	11:25	Removal of malachite green from aqueous solutions using a modified chitosan composite.					
30708008	4	27	theme	reaction	519:526	arg1	conditions					528:537	The reaction conditions	515:537	The reaction conditions for the adsorption studies	515:564	The reaction conditions for the adsorption studies were optimized based on initial dye concentration, dose rate, reaction time, pH and temperature.					
30708008	1	28	dep	sulphate	209:216	arg1	surfactant					235:244	an anionic surfactant	224:244	an anionic surfactant	224:244	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	1	28	dep	sulphate	209:216	arg1	SDS					219:221	SDS	219:221	SDS	219:221	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	3	29	theme	waste	474:478	arg1	water					480:484	waste water	474:484	waste water	474:484	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	5	30	theme	adsorption	742:751	arg1	mechanism					729:737	the mechanism	725:737	the mechanism of adsorption	725:751	Langmuir and Freundlich isotherm models were adopted to study the mechanism of adsorption.					
30708008	0	31	theme	aqueous	32:38	arg1	solutions					40:48	aqueous solutions	32:48	aqueous solutions	32:48	Removal of malachite green from aqueous solutions using a modified chitosan composite.					
30708008	3	32	theme	toxic	428:432	arg1	dye					443:445	a toxic cationic dye	426:445	a toxic cationic dye	426:445	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	3	32	theme	toxic	428:432	arg1	green					458:462	malachite green	448:462	malachite green (MG)	448:467	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	2	33	theme	IR	338:339	arg1	spectroscopy					341:352	IR spectroscopy	338:352	IR spectroscopy	338:352	The characterisation of the composite was done using SEM, XRD, UV-visible and IR spectroscopy studies.					
30708008	7	34	theme	effective	911:919	arg1	composite					889:897	the CPAC based composite	874:897	the CPAC based composite	874:897	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	7	34	theme	effective	911:919	arg1	cost					925:928	an effective low cost	908:928	an effective low cost adsorbent for the removal of MG from waste water	908:977	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	2	35	theme	spectroscopy	341:352	arg1	studies					354:360	SEM, XRD, UV-visible and IR spectroscopy studies	313:360	SEM, XRD, UV-visible and IR spectroscopy studies	313:360	The characterisation of the composite was done using SEM, XRD, UV-visible and IR spectroscopy studies.					
30708008	3	36	theme	cationic	434:441	arg1	dye					443:445	a toxic cationic dye	426:445	a toxic cationic dye	426:445	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	3	36	theme	cationic	434:441	arg1	green					458:462	malachite green	448:462	malachite green (MG)	448:467	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	6	37	theme	adsorption	758:767	arg1	process					769:775	The adsorption process	754:775	The adsorption process	754:775	The adsorption process was found to follow pseudo second order kinetics.					
30708008	7	38	theme	low	921:923	arg1	composite					889:897	the CPAC based composite	874:897	the CPAC based composite	874:897	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	7	38	theme	low	921:923	arg1	cost					925:928	an effective low cost	908:928	an effective low cost adsorbent for the removal of MG from waste water	908:977	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	3	39	theme	adsorption	495:504	arg1	studies					506:512	adsorption studies	495:512	adsorption studies	495:512	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	3	40	theme	dye	443:445	arg1	removal					415:421	the removal	411:421	the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies	411:512	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	1	41	theme	novel	106:110	arg1	composite					112:120	a novel composite adsorbent	104:130	a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant)	104:245	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	4	42	theme	dye	598:600	arg1	concentration					602:614	initial dye concentration	590:614	initial dye concentration	590:614	The reaction conditions for the adsorption studies were optimized based on initial dye concentration, dose rate, reaction time, pH and temperature.					
30708008	1	43	theme	composite	112:120	arg1	synthesis					91:99	The synthesis	87:99	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant)	87:245	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	1	44	theme	sodium	194:199	arg1	sulphate					209:216	sodium dodecyl sulphate	194:216	sodium dodecyl sulphate (SDS, an anionic surfactant)	194:245	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	7	45	theme	adsorbent	930:938	arg1	composite					889:897	the CPAC based composite	874:897	the CPAC based composite	874:897	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	7	45	theme	adsorbent	930:938	arg1	cost					925:928	an effective low cost	908:928	an effective low cost adsorbent for the removal of MG from waste water	908:977	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	1	46	theme	adsorbent	122:130	arg1	composite					112:120	a novel composite adsorbent	104:130	a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant)	104:245	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	3	47	from	water	480:484	arg1	removal					415:421	the removal	411:421	the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies	411:512	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	1	48	theme	dodecyl	201:207	arg1	sulphate					209:216	sodium dodecyl sulphate	194:216	sodium dodecyl sulphate (SDS, an anionic surfactant)	194:245	The synthesis of a novel composite adsorbent prepared from coir pith activated carbon (CPAC), chitosan and sodium dodecyl sulphate (SDS, an anionic surfactant) is reported.					
30708008	0	49	theme	modified	58:65	arg1	composite					76:84	a modified chitosan composite	56:84	a modified chitosan composite	56:84	Removal of malachite green from aqueous solutions using a modified chitosan composite.					
30708008	2	50	theme	XRD	318:320	arg1	studies					354:360	SEM, XRD, UV-visible and IR spectroscopy studies	313:360	SEM, XRD, UV-visible and IR spectroscopy studies	313:360	The characterisation of the composite was done using SEM, XRD, UV-visible and IR spectroscopy studies.					
30708008	5	51	theme	Langmuir	663:670	arg1	models					696:701	Langmuir and Freundlich isotherm models	663:701	Langmuir and Freundlich isotherm models	663:701	Langmuir and Freundlich isotherm models were adopted to study the mechanism of adsorption.					
30708008	7	52	theme	CPAC	878:881	arg1	composite					889:897	the CPAC based composite	874:897	the CPAC based composite	874:897	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	7	52	theme	CPAC	878:881	arg1	cost					925:928	an effective low cost	908:928	an effective low cost adsorbent for the removal of MG from waste water	908:977	The results of the present study indicate that the CPAC based composite could be an effective low cost adsorbent for the removal of MG from waste water.					
30708008	3	53	theme	malachite	448:456	arg1	MG					465:466	MG	465:466	MG	465:466	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	3	53	theme	malachite	448:456	arg1	dye					443:445	a toxic cationic dye	426:445	a toxic cationic dye	426:445	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30708008	3	53	theme	malachite	448:456	arg1	green					458:462	malachite green	448:462	malachite green (MG)	448:467	The effectiveness of the composite was made for the removal of a toxic cationic dye, malachite green (MG) from waste water based on adsorption studies.					
30308944	5	0	from	increase	1176:1183	arg1	production					1199:1208	propionate production	1188:1208	propionate production	1188:1208	Beta-glucan induced the growth of Prevotella and Roseburia with a concomitant increase in propionate production.					
30308944	0	1	from	Fermentation	9:20	arg1	Activity					86:93	Activity	86:93	Activity	86:93	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	0	1	from	Fermentation	9:20	arg1	Composition					70:80	Composition	70:80	Composition	70:80	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	3	2	theme	screening	500:508	arg1	platform					510:517	An in vitro fermentation screening platform	475:517	An in vitro fermentation screening platform (i-screen)	475:528	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	2	theme	screening	500:508	arg1	i-screen					520:527	i-screen	520:527	i-screen	520:527	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	4	3	theme	distinct	1007:1014	arg1	effects					1016:1022	more distinct effects	1002:1022	more distinct effects on the microbial composition and metabolism	1002:1066	All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers.					
30308944	3	4	dep	inulin	646:651	arg1	GOS					717:719	beta-linked GOS	705:719	beta-linked GOS	705:719	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	4	dep	inulin	646:651	arg1	xylo-oligosaccharides					722:742	xylo-oligosaccharides	722:742	xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane	722:789	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	4	dep	inulin	646:651	arg1	alpha-GOS					693:701	alpha-GOS	693:701	alpha-GOS	693:701	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	4	dep	inulin	646:651	arg1	beta-glucan					796:806	beta-glucan	796:806	beta-glucan	796:806	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	4	dep	inulin	646:651	arg1	XOS					745:747	XOS	745:747	XOS	745:747	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	4	dep	inulin	646:651	arg1	galacto-oligosaccharides					667:690	alpha-linked galacto-oligosaccharides	654:690	alpha-linked galacto-oligosaccharides (alpha-GOS)	654:702	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	4	5	theme	dietary	932:938	arg1	fibers					940:945	All dietary fibers	928:945	All dietary fibers	928:945	All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers.					
30308944	3	6	from	cobs	760:763	arg1	GOS					717:719	beta-linked GOS	705:719	beta-linked GOS	705:719	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	6	from	cobs	760:763	arg1	xylo-oligosaccharides					722:742	xylo-oligosaccharides	722:742	xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane	722:789	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	6	from	cobs	760:763	arg1	alpha-GOS					693:701	alpha-GOS	693:701	alpha-GOS	693:701	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	6	from	cobs	760:763	arg1	beta-glucan					796:806	beta-glucan	796:806	beta-glucan	796:806	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	6	from	cobs	760:763	arg1	XOS					745:747	XOS	745:747	XOS	745:747	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	6	from	cobs	760:763	arg1	galacto-oligosaccharides					667:690	alpha-linked galacto-oligosaccharides	654:690	alpha-linked galacto-oligosaccharides (alpha-GOS)	654:702	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	7	dep	concentrations	630:643	arg1	inulin					646:651	inulin	646:651	inulin	646:651	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	8	8	theme	study	1420:1424	arg1	findings					1403:1410	The findings	1399:1410	The findings of this study	1399:1424	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	8	9	theme	potential	1646:1654	arg1	benefits					1663:1670	potential health benefits	1646:1670	potential health benefits	1646:1670	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	1	10	theme	additional	334:343	arg1	bifidobacteria					349:362	bifidobacteria	349:362	bifidobacteria	349:362	Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli.					
30308944	6	11	theme	XOS	1244:1246	arg1	Inulin					1211:1216	Inulin	1211:1216	Inulin	1211:1216	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	6	11	theme	XOS	1244:1246	arg1	forms					1227:1231	both forms	1222:1231	both forms of GOS and XOS	1222:1246	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	8	12	theme	due	1518:1520	arg1	prebiotics					1506:1515	novel prebiotics	1500:1515	novel prebiotics	1500:1515	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	3	13	theme	positive	838:845	arg1	control					876:882	a positive fructo-oligosaccharide (FOS) control	836:882	a positive fructo-oligosaccharide (FOS) control	836:882	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	14	dep	in	478:479	arg1	vitro					481:485	vitro	481:485	vitro	481:485	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	15	link	beta-linked	705:715	arg1	GOS					717:719	beta-linked GOS	705:719	beta-linked GOS	705:719	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	1	16	theme	selective	254:262	arg1	stimulation					264:274	selective stimulation	254:274	selective stimulation	254:274	Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli.					
30308944	1	17	dep	other	318:322	arg1	than					324:327	than	324:327	than	324:327	Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli.					
30308944	6	18	theme	GOS	1236:1238	arg1	Inulin					1211:1216	Inulin	1211:1216	Inulin	1211:1216	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	6	18	theme	GOS	1236:1238	arg1	forms					1227:1231	both forms	1222:1231	both forms of GOS and XOS	1222:1246	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	8	19	from	shifts	1561:1566	arg1	production					1621:1630	short-chain fatty acid production	1598:1630	short-chain fatty acid production	1598:1630	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	8	19	from	shifts	1561:1566	arg1	composition					1582:1592	microbiome composition	1571:1592	microbiome composition	1571:1592	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	8	20	theme	health	1656:1661	arg1	benefits					1663:1670	potential health benefits	1646:1670	potential health benefits	1646:1670	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	8	21	theme	positive	1552:1559	arg1	shifts					1561:1566	the positive shifts	1548:1566	the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits	1548:1670	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	5	22	theme	propionate	1188:1197	arg1	production					1199:1208	propionate production	1188:1208	propionate production	1188:1208	Beta-glucan induced the growth of Prevotella and Roseburia with a concomitant increase in propionate production.					
30308944	3	23	theme	beta-linked	705:715	arg1	GOS					717:719	beta-linked GOS	705:719	beta-linked GOS	705:719	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	4	24	theme	prebiotic	957:965	arg1	activity					967:974	prebiotic activity	957:974	prebiotic activity	957:974	All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers.					
30308944	3	25	theme	FOS	871:873	arg1	control					876:882	a positive fructo-oligosaccharide (FOS) control	836:882	a positive fructo-oligosaccharide (FOS) control	836:882	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	1	26	theme	prebiotics	149:158	arg1	concept					138:144	the concept	134:144	the concept of prebiotics	134:158	Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli.					
30308944	2	27	theme	novel	444:448	arg1	prebiotics					450:459	well-known and novel prebiotics	429:459	well-known and novel prebiotics	429:459	Here, the gut microbiota-modulating effects of well-known and novel prebiotics were studied.					
30308944	8	28	theme	novel	1500:1504	arg1	prebiotics					1506:1515	novel prebiotics	1500:1515	novel prebiotics	1500:1515	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	0	29	dep	Composition	70:80	arg1	the					66:68	the	66:68	the	66:68	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	8	30	theme	oat	1472:1474	arg1	beta-glucan					1476:1486	oat beta-glucan	1472:1486	oat beta-glucan	1472:1486	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	0	31	theme	In	0:1	arg1	Fermentation					9:20	In Vitro Fermentation	0:20	In Vitro Fermentation of Selected Prebiotics	0:43	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	5	32	theme	concomitant	1164:1174	arg1	increase					1176:1183	a concomitant increase	1162:1183	a concomitant increase in propionate production	1162:1208	Beta-glucan induced the growth of Prevotella and Roseburia with a concomitant increase in propionate production.					
30308944	3	33	theme	high-fiber	769:778	arg1	cane					786:789	high-fiber sugar cane	769:789	high-fiber sugar cane	769:789	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	8	34	theme	microbiome	1571:1580	arg1	composition					1582:1592	microbiome composition	1571:1592	microbiome composition	1571:1592	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	2	35	theme	well-known	429:438	arg1	prebiotics					450:459	well-known and novel prebiotics	429:459	well-known and novel prebiotics	429:459	Here, the gut microbiota-modulating effects of well-known and novel prebiotics were studied.					
30308944	5	36	theme	Roseburia	1147:1155	arg1	growth					1122:1127	the growth	1118:1127	the growth of Prevotella and Roseburia	1118:1155	Beta-glucan induced the growth of Prevotella and Roseburia with a concomitant increase in propionate production.					
30308944	0	37	theme	Gut	108:110	arg1	Microbiota					112:121	the Adult Gut Microbiota	98:121	the Adult Gut Microbiota	98:121	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	7	38	theme	dose-response	1312:1324	arg1	effect					1326:1331	A dose-response effect	1310:1331	A dose-response effect	1310:1331	A dose-response effect was observed for butyrate when exposed to beta-glucan and inulin.					
30308944	3	39	theme	adult	550:554	arg1	microbiota					562:571	adult fecal microbiota	550:571	adult fecal microbiota	550:571	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	0	40	theme	Adult	102:106	arg1	Microbiota					112:121	the Adult Gut Microbiota	98:121	the Adult Gut Microbiota	98:121	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	3	41	theme	concentrations	630:643	arg1	range					621:625	a range	619:625	a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats)	619:817	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	42	theme	fecal	556:560	arg1	microbiota					562:571	adult fecal microbiota	550:571	adult fecal microbiota	550:571	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	4	43	theme	other	1084:1088	arg1	fibers					1090:1095	the other fibers	1080:1095	the other fibers	1080:1095	All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers.					
30308944	2	44	theme	microbiota-modulating	396:416	arg1	effects					418:424	the gut microbiota-modulating effects	388:424	the gut microbiota-modulating effects of well-known and novel prebiotics	388:459	Here, the gut microbiota-modulating effects of well-known and novel prebiotics were studied.					
30308944	0	45	theme	Prebiotics	34:43	arg1	Fermentation					9:20	In Vitro Fermentation	0:20	In Vitro Fermentation of Selected Prebiotics	0:43	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	0	45	theme	Prebiotics	34:43	arg1	Effects					55:61	Their Effects	49:61	Their Effects on the Composition and Activity of the Adult Gut Microbiota	49:121	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	3	46	from	oats	813:816	arg1	GOS					717:719	beta-linked GOS	705:719	beta-linked GOS	705:719	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	46	from	oats	813:816	arg1	xylo-oligosaccharides					722:742	xylo-oligosaccharides	722:742	xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane	722:789	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	46	from	oats	813:816	arg1	alpha-GOS					693:701	alpha-GOS	693:701	alpha-GOS	693:701	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	46	from	oats	813:816	arg1	beta-glucan					796:806	beta-glucan	796:806	beta-glucan	796:806	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	46	from	oats	813:816	arg1	XOS					745:747	XOS	745:747	XOS	745:747	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	46	from	oats	813:816	arg1	galacto-oligosaccharides					667:690	alpha-linked galacto-oligosaccharides	654:690	alpha-linked galacto-oligosaccharides (alpha-GOS)	654:702	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	8	47	theme	short-chain	1598:1608	arg1	acid					1616:1619	short-chain fatty acid	1598:1619	short-chain fatty acid production	1598:1630	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	3	48	theme	different	585:593	arg1	fibers					603:608	different dietary fibers	585:608	different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats)	585:817	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	49	theme	negative	890:897	arg1	addition					917:924	no fiber addition	908:924	no fiber addition	908:924	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	49	theme	negative	890:897	arg1	control					899:905	a negative control	888:905	a negative control (no fiber addition)	888:925	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	2	50	theme	gut	392:394	arg1	effects					418:424	the gut microbiota-modulating effects	388:424	the gut microbiota-modulating effects of well-known and novel prebiotics	388:459	Here, the gut microbiota-modulating effects of well-known and novel prebiotics were studied.					
30308944	0	51	theme	Selected	25:32	arg1	Prebiotics					34:43	Selected Prebiotics	25:43	Selected Prebiotics	25:43	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	0	52	theme	Microbiota	112:121	arg1	Activity					86:93	Activity	86:93	Activity	86:93	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	0	52	theme	Microbiota	112:121	arg1	Composition					70:80	Composition	70:80	Composition	70:80	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	6	53	theme	microbial	1287:1295	arg1	composition					1297:1307	the microbial composition	1283:1307	the microbial composition	1283:1307	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	3	54	theme	dietary	595:601	arg1	fibers					603:608	different dietary fibers	585:608	different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats)	585:817	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	1	55	theme	microbial	301:309	arg1	groups					311:316	microbial groups	301:316	microbial groups other than, and additional to, bifidobacteria and lactobacilli	301:379	Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli.					
30308944	0	56	from	Effects	55:61	arg1	Activity					86:93	Activity	86:93	Activity	86:93	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	0	56	from	Effects	55:61	arg1	Composition					70:80	Composition	70:80	Composition	70:80	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	2	57	theme	prebiotics	450:459	arg1	effects					418:424	the gut microbiota-modulating effects	388:424	the gut microbiota-modulating effects of well-known and novel prebiotics	388:459	Here, the gut microbiota-modulating effects of well-known and novel prebiotics were studied.					
30308944	3	58	theme	corn	755:758	arg1	cobs					760:763	corn cobs	755:763	corn cobs	755:763	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	59	from	cane	786:789	arg1	GOS					717:719	beta-linked GOS	705:719	beta-linked GOS	705:719	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	59	from	cane	786:789	arg1	xylo-oligosaccharides					722:742	xylo-oligosaccharides	722:742	xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane	722:789	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	59	from	cane	786:789	arg1	alpha-GOS					693:701	alpha-GOS	693:701	alpha-GOS	693:701	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	59	from	cane	786:789	arg1	beta-glucan					796:806	beta-glucan	796:806	beta-glucan	796:806	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	59	from	cane	786:789	arg1	XOS					745:747	XOS	745:747	XOS	745:747	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	59	from	cane	786:789	arg1	galacto-oligosaccharides					667:690	alpha-linked galacto-oligosaccharides	654:690	alpha-linked galacto-oligosaccharides (alpha-GOS)	654:702	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	60	theme	alpha-linked	654:665	arg1	alpha-GOS					693:701	alpha-GOS	693:701	alpha-GOS	693:701	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	60	theme	alpha-linked	654:665	arg1	galacto-oligosaccharides					667:690	alpha-linked galacto-oligosaccharides	654:690	alpha-linked galacto-oligosaccharides (alpha-GOS)	654:702	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	4	61	from	effects	1016:1022	arg1	composition					1041:1051	the microbial composition	1027:1051	the microbial composition	1027:1051	All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers.					
30308944	4	61	from	effects	1016:1022	arg1	metabolism					1057:1066	metabolism	1057:1066	metabolism	1057:1066	All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers.					
30308944	3	62	theme	no	908:909	arg1	addition					917:924	no fiber addition	908:924	no fiber addition	908:924	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	62	theme	no	908:909	arg1	control					899:905	a negative control	888:905	a negative control (no fiber addition)	888:925	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	63	theme	fermentation	487:498	arg1	platform					510:517	An in vitro fermentation screening platform	475:517	An in vitro fermentation screening platform (i-screen)	475:528	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	63	theme	fermentation	487:498	arg1	i-screen					520:527	i-screen	520:527	i-screen	520:527	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	8	64	theme	fatty	1610:1614	arg1	acid					1616:1619	short-chain fatty acid	1598:1619	short-chain fatty acid production	1598:1630	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	5	65	theme	Prevotella	1132:1141	arg1	growth					1122:1127	the growth	1118:1127	the growth of Prevotella and Roseburia	1118:1155	Beta-glucan induced the growth of Prevotella and Roseburia with a concomitant increase in propionate production.					
30308944	6	66	theme	bifidogenic	1261:1271	arg1	effect					1273:1278	a strong bifidogenic effect	1252:1278	a strong bifidogenic effect	1252:1278	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	8	67	with	association	1531:1541	arg1	shifts					1561:1566	the positive shifts	1548:1566	the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits	1548:1670	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	3	68	theme	fiber	911:915	arg1	addition					917:924	no fiber addition	908:924	no fiber addition	908:924	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	68	theme	fiber	911:915	arg1	control					899:905	a negative control	888:905	a negative control (no fiber addition)	888:925	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	4	69	theme	microbial	1031:1039	arg1	composition					1041:1051	the microbial composition	1027:1051	the microbial composition	1027:1051	All dietary fibers displayed prebiotic activity, with beta-glucan showing more distinct effects on the microbial composition and metabolism compared to the other fibers.					
30308944	1	70	theme	non-digestible	196:209	arg1	oligosaccharides					211:226	non-digestible oligosaccharides	196:226	non-digestible oligosaccharides	196:226	Recently, the concept of prebiotics has been revisited to expand beyond non-digestible oligosaccharides, and the requirements for selective stimulation were extended to include microbial groups other than, and additional to, bifidobacteria and lactobacilli.					
30308944	6	71	theme	strong	1254:1259	arg1	effect					1273:1278	a strong bifidogenic effect	1252:1278	a strong bifidogenic effect	1252:1278	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	3	72	theme	in	478:479	arg1	platform					510:517	An in vitro fermentation screening platform	475:517	An in vitro fermentation screening platform (i-screen)	475:528	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	72	theme	in	478:479	arg1	i-screen					520:527	i-screen	520:527	i-screen	520:527	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	73	link	alpha-linked	654:665	arg1	alpha-GOS					693:701	alpha-GOS	693:701	alpha-GOS	693:701	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	73	link	alpha-linked	654:665	arg1	galacto-oligosaccharides					667:690	alpha-linked galacto-oligosaccharides	654:690	alpha-linked galacto-oligosaccharides (alpha-GOS)	654:702	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	6	74	contain	had	1248:1250	arg1	Inulin					1211:1216	Inulin	1211:1216	Inulin	1211:1216	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	6	74	contain	had	1248:1250	arg1	forms					1227:1231	both forms	1222:1231	both forms of GOS and XOS	1222:1246	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	6	74	contain	had	1248:1250	arg2	effect					1273:1278	a strong bifidogenic effect	1252:1278	a strong bifidogenic effect	1252:1278	Inulin and both forms of GOS and XOS had a strong bifidogenic effect on the microbial composition.					
30308944	8	75	theme	acid	1616:1619	arg1	production					1621:1630	short-chain fatty acid production	1598:1630	short-chain fatty acid production	1598:1630	The findings of this study support the potential for alpha-GOS, XOS, and oat beta-glucan to serve as novel prebiotics, due to their association with the positive shifts in microbiome composition and short-chain fatty acid production that point to potential health benefits.					
30308944	3	76	theme	sugar	780:784	arg1	cane					786:789	high-fiber sugar cane	769:789	high-fiber sugar cane	769:789	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	0	77	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Fermentation of Selected Prebiotics and Their Effects on the Composition and Activity of the Adult Gut Microbiota.					
30308944	3	78	theme	fructo-oligosaccharide	847:868	arg1	control					876:882	a positive fructo-oligosaccharide (FOS) control	836:882	a positive fructo-oligosaccharide (FOS) control	836:882	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	79	contain	had	615:617	arg2	range					621:625	a range	619:625	a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats)	619:817	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
30308944	3	79	contain	had	615:617	arg1	fibers					603:608	different dietary fibers	585:608	different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats)	585:817	An in vitro fermentation screening platform (i-screen) was inoculated with adult fecal microbiota, exposed to different dietary fibers that had a range of concentrations (inulin, alpha-linked galacto-oligosaccharides (alpha-GOS), beta-linked GOS, xylo-oligosaccharides (XOS) from corn cobs and high-fiber sugar cane, and beta-glucan from oats), and compared to a positive fructo-oligosaccharide (FOS) control and a negative control (no fiber addition).					
31589660	5	0	from	4b	1021:1022	arg1	loss					915:918	a loss	913:918	a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules	913:992	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	5	0	from	4b	1021:1022	arg1	switch					1001:1006	a switch	999:1006	a switch from serovar 4b to 4d	999:1028	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	9	1	theme	crucial	1732:1738	arg1	antigen					1724:1730	a surface antigen	1714:1730	a surface antigen crucial for both phage adsorption and cellular invasiveness	1714:1790	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	4	2	theme	exact	718:722	arg1	nature					724:729	the exact nature	714:729	the exact nature of the 4b-specific antigen	714:756	Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen.					
31589660	1	3	theme	pathogen	162:169	arg1	monocytogenes					180:192	The intracellular pathogen Listeria monocytogenes	144:192	The intracellular pathogen Listeria monocytogenes	144:192	The intracellular pathogen Listeria monocytogenes is distinguished by its ability to invade and replicate within mammalian cells.					
31589660	9	4	theme	opportunistic	1866:1878	arg1	pathogen					1880:1887	this opportunistic pathogen	1861:1887	this opportunistic pathogen	1861:1887	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	0	5	theme	serovar	66:72	arg1	4b					74:75	serovar 4b	66:75	virulence: Listeria monocytogenes serovar 4b	32:75	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	7	6	theme	reduced	1428:1434	arg1	invasiveness					1445:1456	a drastically reduced cellular invasiveness	1414:1456	a drastically reduced cellular invasiveness	1414:1456	We show that InlB specifically recognizes and attaches to galactosylated teichoic acid polymers, and is secreted upon loss of this modification, leading to a drastically reduced cellular invasiveness.					
31589660	5	7	from	loss	915:918	arg1	molecules					984:992	both wall teichoic acid and lipoteichoic acid molecules	938:992	both wall teichoic acid and lipoteichoic acid molecules	938:992	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	1	8	theme	Listeria	171:178	arg1	monocytogenes					180:192	The intracellular pathogen Listeria monocytogenes	144:192	The intracellular pathogen Listeria monocytogenes	144:192	The intracellular pathogen Listeria monocytogenes is distinguished by its ability to invade and replicate within mammalian cells.					
31589660	8	9	theme	cellular	1594:1601	arg1	uptake					1603:1608	cellular uptake	1594:1608	cellular uptake	1594:1608	Consequently, these phage-insensitive bacteria are unable to interact with cMet and gC1q-R host cell receptors, which normally trigger cellular uptake upon interaction with InlB.					
31589660	0	10	theme	galactosylated	86:99	arg1	acids					110:114	galactosylated teichoic acids	86:114	galactosylated teichoic acids	86:114	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	7	11	theme	teichoic	1331:1338	arg1	polymers					1345:1352	galactosylated teichoic acid polymers	1316:1352	galactosylated teichoic acid polymers	1316:1352	We show that InlB specifically recognizes and attaches to galactosylated teichoic acid polymers, and is secreted upon loss of this modification, leading to a drastically reduced cellular invasiveness.					
31589660	6	12	theme	attenuation	1237:1247	arg1	loss					1139:1142	a complete loss	1128:1142	a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation	1128:1247	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	5	13	theme	acid	979:982	arg1	molecules					984:992	both wall teichoic acid and lipoteichoic acid molecules	938:992	both wall teichoic acid and lipoteichoic acid molecules	938:992	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	5	14	from	molecules	984:992	arg1	loss					915:918	a loss	913:918	a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules	913:992	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	5	14	from	molecules	984:992	arg1	switch					1001:1006	a switch	999:1006	a switch from serovar 4b to 4d	999:1028	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	5	14	from	molecules	984:992	arg1	galactose					923:931	galactose	923:931	galactose from both wall teichoic acid and lipoteichoic acid molecules	923:992	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	8	15	with	interaction	1615:1625	arg1	InlB					1632:1635	InlB	1632:1635	InlB	1632:1635	Consequently, these phage-insensitive bacteria are unable to interact with cMet and gC1q-R host cell receptors, which normally trigger cellular uptake upon interaction with InlB.					
31589660	5	16	from	mutations	842:850	arg1	genes					855:859	genes	855:859	genes involved in teichoic acid glycosylation	855:899	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	6	17	theme	virulence	1227:1235	arg1	attenuation					1237:1247	a virulence attenuation	1225:1247	a virulence attenuation	1225:1247	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	3	18	theme	specific	563:570	arg1	patterns					586:593	their specific glycosylation patterns	557:593	their specific glycosylation patterns	557:593	The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns.					
31589660	5	19	theme	acid	882:885	arg1	glycosylation					887:899	teichoic acid glycosylation	873:899	teichoic acid glycosylation	873:899	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	3	20	theme	peptidoglycan-associated	500:523	arg1	WTAs					546:549	WTAs	546:549	WTAs	546:549	The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns.					
31589660	3	20	theme	peptidoglycan-associated	500:523	arg1	acids					539:543	peptidoglycan-associated wall-teichoic acids	500:543	the peptidoglycan-associated wall-teichoic acids (WTAs)	496:550	The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns.					
31589660	6	21	theme	Internalin	1166:1175	arg1	InlB					1180:1183	InlB	1180:1183	InlB	1180:1183	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	6	21	theme	Internalin	1166:1175	arg1	B					1177:1177	surface-associated Internalin B	1147:1177	surface-associated Internalin B (InlB)	1147:1184	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	2	22	theme	listeriosis	377:387	arg1	cases					398:402	listeriosis clinical cases	377:402	listeriosis clinical cases	377:402	Remarkably, of the 15 serovars within the genus, strains belonging to serovar 4b cause the majority of listeriosis clinical cases and outbreaks.					
31589660	2	23	theme	cases	398:402	arg1	majority					365:372	the majority	361:372	the majority of listeriosis clinical cases and outbreaks	361:416	Remarkably, of the 15 serovars within the genus, strains belonging to serovar 4b cause the majority of listeriosis clinical cases and outbreaks.					
31589660	0	24	theme	teichoic	101:108	arg1	acids					110:114	galactosylated teichoic acids	86:114	galactosylated teichoic acids	86:114	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	6	25	theme	complete	1130:1137	arg1	loss					1139:1142	a complete loss	1128:1142	a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation	1128:1247	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	5	26	theme	feature	834:840	arg1	mutations					842:850	feature mutations	834:850	feature mutations in genes involved in teichoic acid glycosylation	834:899	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	2	27	theme	clinical	389:396	arg1	cases					398:402	listeriosis clinical cases	377:402	listeriosis clinical cases	377:402	Remarkably, of the 15 serovars within the genus, strains belonging to serovar 4b cause the majority of listeriosis clinical cases and outbreaks.					
31589660	9	28	theme	cellular	1770:1777	arg1	invasiveness					1779:1790	cellular invasiveness	1770:1790	cellular invasiveness	1770:1790	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	0	29	from	cost	24:27	arg1	resistance					6:15	Phage resistance	0:15	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b	0:75	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	9	30	theme	surface	1716:1722	arg1	antigen					1724:1730	a surface antigen	1714:1730	a surface antigen crucial for both phage adsorption and cellular invasiveness	1714:1790	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	5	31	theme	teichoic	948:955	arg1	acid					957:960	wall teichoic acid	943:960	wall teichoic acid	943:960	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	5	32	from	switch	1001:1006	arg1	molecules					984:992	both wall teichoic acid and lipoteichoic acid molecules	938:992	both wall teichoic acid and lipoteichoic acid molecules	938:992	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	3	33	theme	Listeria	423:430	arg1	O-antigens					432:441	The Listeria O-antigens	419:441	The Listeria O-antigens	419:441	The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns.					
31589660	0	34	theme	Phage	0:4	arg1	resistance					6:15	Phage resistance	0:15	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b	0:75	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	7	35	attach	attaches	1304:1311	arg1	polymers					1345:1352	galactosylated teichoic acid polymers	1316:1352	galactosylated teichoic acid polymers	1316:1352	We show that InlB specifically recognizes and attaches to galactosylated teichoic acid polymers, and is secreted upon loss of this modification, leading to a drastically reduced cellular invasiveness.					
31589660	7	35	attach	attaches	1304:1311	arg2	InlB					1271:1274	InlB	1271:1274	InlB	1271:1274	We show that InlB specifically recognizes and attaches to galactosylated teichoic acid polymers, and is secreted upon loss of this modification, leading to a drastically reduced cellular invasiveness.					
31589660	4	36	theme	antigen	750:756	arg1	nature					724:729	the exact nature	714:729	the exact nature of the 4b-specific antigen	714:756	Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen.					
31589660	9	37	theme	phage	1749:1753	arg1	adsorption					1755:1764	phage adsorption	1749:1764	phage adsorption	1749:1764	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	0	38	theme	InlB-mediated	120:132	arg1	invasion					134:141	InlB-mediated invasion	120:141	InlB-mediated invasion	120:141	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	3	39	theme	glycosylation	572:584	arg1	patterns					586:593	their specific glycosylation patterns	557:593	their specific glycosylation patterns	557:593	The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns.					
31589660	3	40	theme	structural	465:474	arg1	differences					476:486	subtle structural differences	458:486	subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns	458:593	The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns.					
31589660	5	41	theme	acid	957:960	arg1	molecules					984:992	both wall teichoic acid and lipoteichoic acid molecules	938:992	both wall teichoic acid and lipoteichoic acid molecules	938:992	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	5	42	theme	lipoteichoic	966:977	arg1	acid					979:982	lipoteichoic acid	966:982	lipoteichoic acid	966:982	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	4	43	from	decoration	655:664	arg1	4b					677:678	serovar 4b L. monocytogenes	669:695	serovar 4b L. monocytogenes	669:695	Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen.					
31589660	4	44	theme	WTA	651:653	arg1	decoration					655:664	WTA decoration	651:664	WTA decoration in serovar 4b L. monocytogenes	651:695	Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen.					
31589660	6	45	theme	galactose	1058:1066	arg1	decoration					1068:1077	this galactose decoration	1053:1077	this galactose decoration	1053:1077	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	8	46	theme	cell	1555:1558	arg1	receptors					1560:1568	host cell receptors	1550:1568	host cell receptors	1550:1568	Consequently, these phage-insensitive bacteria are unable to interact with cMet and gC1q-R host cell receptors, which normally trigger cellular uptake upon interaction with InlB.					
31589660	9	47	theme	detailed	1663:1670	arg1	insight					1684:1690	detailed mechanistic insight	1663:1690	detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness	1663:1790	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	4	48	theme	serovar	669:675	arg1	4b					677:678	serovar 4b L. monocytogenes	669:695	serovar 4b L. monocytogenes	669:695	Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen.					
31589660	6	49	theme	actin	1208:1212	arg1	tails					1214:1218	actin tails	1208:1218	actin tails	1208:1218	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	2	50	theme	outbreaks	408:416	arg1	majority					365:372	the majority	361:372	the majority of listeriosis clinical cases and outbreaks	361:416	Remarkably, of the 15 serovars within the genus, strains belonging to serovar 4b cause the majority of listeriosis clinical cases and outbreaks.					
31589660	4	51	theme	L.	680:681	arg1	4b					677:678	serovar 4b L. monocytogenes	669:695	serovar 4b L. monocytogenes	669:695	Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen.					
31589660	8	52	theme	host	1550:1553	arg1	receptors					1560:1568	host cell receptors	1550:1568	host cell receptors	1550:1568	Consequently, these phage-insensitive bacteria are unable to interact with cMet and gC1q-R host cell receptors, which normally trigger cellular uptake upon interaction with InlB.					
31589660	3	53	theme	subtle	458:463	arg1	differences					476:486	subtle structural differences	458:486	subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns	458:593	The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns.					
31589660	5	54	theme	surviving	812:820	arg1	clones					822:827	surviving clones	812:827	surviving clones that feature mutations in genes involved in teichoic acid glycosylation	812:899	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	9	55	theme	dual	1701:1704	arg1	role					1706:1709	the dual role	1697:1709	the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness	1697:1790	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	9	56	theme	antigen	1724:1730	arg1	role					1706:1709	the dual role	1697:1709	the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness	1697:1790	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	7	57	theme	acid	1340:1343	arg1	polymers					1345:1352	galactosylated teichoic acid polymers	1316:1352	galactosylated teichoic acid polymers	1316:1352	We show that InlB specifically recognizes and attaches to galactosylated teichoic acid polymers, and is secreted upon loss of this modification, leading to a drastically reduced cellular invasiveness.					
31589660	6	58	theme	phage	1097:1101	arg1	adsorption					1103:1112	phage adsorption	1097:1112	phage adsorption	1097:1112	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	2	59	theme	serovar	344:350	arg1	4b					352:353	serovar 4b	344:353	serovar 4b	344:353	Remarkably, of the 15 serovars within the genus, strains belonging to serovar 4b cause the majority of listeriosis clinical cases and outbreaks.					
31589660	0	60	theme	virulence	32:40	arg1	monocytogenes					52:64	virulence: Listeria monocytogenes serovar 4b	32:75	virulence: Listeria monocytogenes serovar 4b	32:75	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	6	61	theme	B	1177:1177	arg1	loss					1139:1142	a complete loss	1128:1142	a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation	1128:1247	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	9	62	theme	mechanistic	1672:1682	arg1	insight					1684:1690	detailed mechanistic insight	1663:1690	detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness	1663:1790	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	6	63	theme	inability	1190:1198	arg1	loss					1139:1142	a complete loss	1128:1142	a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation	1128:1247	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	3	64	theme	wall-teichoic	525:537	arg1	WTAs					546:549	WTAs	546:549	WTAs	546:549	The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns.					
31589660	3	64	theme	wall-teichoic	525:537	arg1	acids					539:543	peptidoglycan-associated wall-teichoic acids	500:543	the peptidoglycan-associated wall-teichoic acids (WTAs)	496:550	The Listeria O-antigens are defined by subtle structural differences amongst the peptidoglycan-associated wall-teichoic acids (WTAs), and their specific glycosylation patterns.					
31589660	7	65	theme	cellular	1436:1443	arg1	invasiveness					1445:1456	a drastically reduced cellular invasiveness	1414:1456	a drastically reduced cellular invasiveness	1414:1456	We show that InlB specifically recognizes and attaches to galactosylated teichoic acid polymers, and is secreted upon loss of this modification, leading to a drastically reduced cellular invasiveness.					
31589660	5	66	theme	serovar	1013:1019	arg1	4b					1021:1022	serovar 4b	1013:1022	serovar 4b	1013:1022	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	8	67	dep	cMet	1534:1537	arg1	receptors					1560:1568	host cell receptors	1550:1568	host cell receptors	1550:1568	Consequently, these phage-insensitive bacteria are unable to interact with cMet and gC1q-R host cell receptors, which normally trigger cellular uptake upon interaction with InlB.					
31589660	7	68	theme	galactosylated	1316:1329	arg1	polymers					1345:1352	galactosylated teichoic acid polymers	1316:1352	galactosylated teichoic acid polymers	1316:1352	We show that InlB specifically recognizes and attaches to galactosylated teichoic acid polymers, and is secreted upon loss of this modification, leading to a drastically reduced cellular invasiveness.					
31589660	9	69	theme	phage	1827:1831	arg1	resistance					1833:1842	phage resistance	1827:1842	phage resistance	1827:1842	Collectively, we provide detailed mechanistic insight into the dual role of a surface antigen crucial for both phage adsorption and cellular invasiveness, demonstrating a trade-off between phage resistance and virulence in this opportunistic pathogen.					
31589660	4	70	theme	4b-specific	738:748	arg1	antigen					750:756	the 4b-specific antigen	734:756	the 4b-specific antigen	734:756	Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen.					
31589660	0	71	dep	monocytogenes	52:64	arg1	4b					74:75	serovar 4b	66:75	virulence: Listeria monocytogenes serovar 4b	32:75	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	6	72	theme	surface-associated	1147:1164	arg1	InlB					1180:1183	InlB	1180:1183	InlB	1180:1183	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	6	72	theme	surface-associated	1147:1164	arg1	B					1177:1177	surface-associated Internalin B	1147:1177	surface-associated Internalin B (InlB)	1147:1184	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	5	73	theme	galactose	923:931	arg1	loss					915:918	a loss	913:918	a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules	913:992	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	5	73	theme	galactose	923:931	arg1	switch					1001:1006	a switch	999:1006	a switch from serovar 4b to 4d	999:1028	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	7	74	theme	modification	1389:1400	arg1	loss					1376:1379	loss	1376:1379	loss	1376:1379	We show that InlB specifically recognizes and attaches to galactosylated teichoic acid polymers, and is secreted upon loss of this modification, leading to a drastically reduced cellular invasiveness.					
31589660	5	75	theme	wall	943:946	arg1	acid					957:960	wall teichoic acid	943:960	wall teichoic acid	943:960	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	4	76	theme	genetic	617:623	arg1	determinants					625:636	the genetic determinants	613:636	the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes	613:695	Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen.					
31589660	0	77	theme	monocytogenes	52:64	arg1	cost					24:27	the cost	20:27	the cost of virulence: Listeria monocytogenes serovar 4b	20:75	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	5	78	theme	teichoic	873:880	arg1	acid					882:885	teichoic acid	873:885	teichoic acid glycosylation	873:899	We show that challenge by bacteriophages selects for surviving clones that feature mutations in genes involved in teichoic acid glycosylation, leading to a loss of galactose from both wall teichoic acid and lipoteichoic acid molecules, and a switch from serovar 4b to 4d.					
31589660	6	79	theme	decoration	1068:1077	arg1	loss					1045:1048	loss	1045:1048	loss of this galactose decoration	1045:1077	Surprisingly, loss of this galactose decoration not only prevents phage adsorption, but leads to a complete loss of surface-associated Internalin B (InlB),the inability to form actin tails, and a virulence attenuation in vivo.					
31589660	0	80	theme	Listeria	43:50	arg1	monocytogenes					52:64	virulence: Listeria monocytogenes serovar 4b	32:75	virulence: Listeria monocytogenes serovar 4b	32:75	Phage resistance at the cost of virulence: Listeria monocytogenes serovar 4b requires galactosylated teichoic acids for InlB-mediated invasion.					
31589660	4	81	dep	4b	677:678	arg1	monocytogenes					683:695	serovar 4b L. monocytogenes	669:695	serovar 4b L. monocytogenes	669:695	Here, we outline the genetic determinants required for WTA decoration in serovar 4b L. monocytogenes, and demonstrate the exact nature of the 4b-specific antigen.					
31589660	1	82	theme	intracellular	148:160	arg1	monocytogenes					180:192	The intracellular pathogen Listeria monocytogenes	144:192	The intracellular pathogen Listeria monocytogenes	144:192	The intracellular pathogen Listeria monocytogenes is distinguished by its ability to invade and replicate within mammalian cells.					
31589660	8	83	theme	phage-insensitive	1479:1495	arg1	bacteria					1497:1504	these phage-insensitive bacteria	1473:1504	these phage-insensitive bacteria	1473:1504	Consequently, these phage-insensitive bacteria are unable to interact with cMet and gC1q-R host cell receptors, which normally trigger cellular uptake upon interaction with InlB.					
31589660	1	84	theme	mammalian	257:265	arg1	cells					267:271	mammalian cells	257:271	mammalian cells	257:271	The intracellular pathogen Listeria monocytogenes is distinguished by its ability to invade and replicate within mammalian cells.					
31789000	0	0	theme	thromboembolism	79:93	arg1	treatment					59:67	the long-term treatment	45:67	the long-term treatment of venous thromboembolism in patients with gynecologic cancers	45:130	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	9	1	theme	rivaroxaban	1352:1362	arg1	group					1364:1368	the rivaroxaban group	1348:1368	the rivaroxaban group	1348:1368	In the rivaroxaban group, 44.0% of patients experienced gastrointestinal bleeding and 24.0% experienced urinary tract bleeding.					
31789000	1	2	from	outcomes	189:196	arg1	patients					201:208	patients	201:208	patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE)	201:331	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	11	3	theme	rivaroxaban	1699:1709	arg1	groups					1726:1731	the rivaroxaban and dalteparin groups	1695:1731	groups	1726:1731	CONCLUSION In this study, although there were no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups, rivaroxaban use was associated with a higher rate of clinically relevant bleeding than dalteparin.					
31789000	10	4	theme	injection	1563:1571	arg1	site					1573:1576	the injection site	1559:1576	the injection site (22.2%)	1559:1584	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	10	4	theme	injection	1563:1571	arg1	%					1583:1583	22.2%	1579:1583	22.2%	1579:1583	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	0	5	theme	venous	72:77	arg1	thromboembolism					79:93	venous thromboembolism	72:93	venous thromboembolism in patients with gynecologic cancers	72:130	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	11	6	theme	rivaroxaban	1734:1744	arg1	use					1746:1748	rivaroxaban use	1734:1748	rivaroxaban use	1734:1748	CONCLUSION In this study, although there were no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups, rivaroxaban use was associated with a higher rate of clinically relevant bleeding than dalteparin.					
31789000	8	7	theme	events	1285:1290	arg1	number					1265:1270	the number	1261:1270	the number of composite events	1261:1290	Multivariate analysis using the Cox proportional hazards model also showed no significant difference in the number of composite events and clinically relevant bleeding between the groups.					
31789000	3	8	theme	cancer-associated	545:561	arg1	VTE					563:565	cancer-associated VTE	545:565	cancer-associated VTE	545:565	We compared the outcomes of patients with primary gynecological cancers who were treated for cancer-associated VTE with either rivaroxaban or dalteparin.					
31789000	11	9	dep	CONCLUSION	1587:1596	arg1	study					1606:1610	this study	1601:1610	this study	1601:1610	CONCLUSION In this study, although there were no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups, rivaroxaban use was associated with a higher rate of clinically relevant bleeding than dalteparin.					
31789000	4	10	theme	eligible	622:629	arg1	patients					631:638	The 162 eligible patients	614:638	METHODS The 162 eligible patients with gynecologic cancers who were treated with either dalteparin (n=60) or rivaroxaban (n=102)	606:733	METHODS The 162 eligible patients with gynecologic cancers who were treated with either dalteparin (n=60) or rivaroxaban (n=102) were reviewed.					
31789000	8	11	from	difference	1247:1256	arg1	bleeding					1316:1323	bleeding	1316:1323	bleeding	1316:1323	Multivariate analysis using the Cox proportional hazards model also showed no significant difference in the number of composite events and clinically relevant bleeding between the groups.					
31789000	8	11	from	difference	1247:1256	arg1	number					1265:1270	the number	1261:1270	the number of composite events	1261:1290	Multivariate analysis using the Cox proportional hazards model also showed no significant difference in the number of composite events and clinically relevant bleeding between the groups.					
31789000	1	12	dep	OBJECTIVES	133:142	arg1	studies					171:177	Two randomized, controlled studies	144:177	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE)	133:331	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	7	13	theme	significant	1030:1040	arg1	differences					1042:1052	no significant differences	1027:1052	no significant differences between the groups	1027:1071	RESULTS During the therapeutic period, there were no significant differences between the groups in the proportion of composite events, recurrence, or clinically relevant bleeding.					
31789000	1	14	theme	cancer-associated	286:302	arg1	VTE					328:330	VTE	328:330	VTE	328:330	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	1	14	theme	cancer-associated	286:302	arg1	thromboembolism					311:325	cancer-associated venous thromboembolism	286:325	cancer-associated venous thromboembolism (VTE)	286:331	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	12	15	theme	cancer-associated	1913:1929	arg1	VTE					1931:1933	gynecologic cancer-associated VTE	1901:1933	gynecologic cancer-associated VTE	1901:1933	Therefore, caution should be taken when prescribing rivaroxaban for gynecologic cancer-associated VTE and bleeding events should be carefully monitored.					
31789000	1	16	theme	venous	304:309	arg1	VTE					328:330	VTE	328:330	VTE	328:330	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	1	16	theme	venous	304:309	arg1	thromboembolism					311:325	cancer-associated venous thromboembolism	286:325	cancer-associated venous thromboembolism (VTE)	286:331	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	0	17	theme	gynecologic	112:122	arg1	cancers					124:130	gynecologic cancers	112:130	gynecologic cancers	112:130	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	6	18	theme	bleeding	945:952	arg1	events					954:959	clinically relevant bleeding events	925:959	clinically relevant bleeding events	925:959	Secondary outcomes were recurrence, clinically relevant bleeding events, and mortality.					
31789000	12	19	theme	bleeding	1939:1946	arg1	events					1948:1953	bleeding events	1939:1953	bleeding events	1939:1953	Therefore, caution should be taken when prescribing rivaroxaban for gynecologic cancer-associated VTE and bleeding events should be carefully monitored.					
31789000	8	20	theme	Cox	1189:1191	arg1	model					1214:1218	the Cox proportional hazards model	1185:1218	the Cox proportional hazards model	1185:1218	Multivariate analysis using the Cox proportional hazards model also showed no significant difference in the number of composite events and clinically relevant bleeding between the groups.					
31789000	5	21	theme	composite	776:784	arg1	outcome					762:768	The primary outcome	750:768	The primary outcome	750:768	The primary outcome was a composite event, which included recurrence or clinically relevant bleeding events during the therapeutic period.					
31789000	5	21	theme	composite	776:784	arg1	event					786:790	a composite event	774:790	a composite event	774:790	The primary outcome was a composite event, which included recurrence or clinically relevant bleeding events during the therapeutic period.					
31789000	4	22	dep	METHODS	606:612	arg1	patients					631:638	The 162 eligible patients	614:638	METHODS The 162 eligible patients with gynecologic cancers who were treated with either dalteparin (n=60) or rivaroxaban (n=102)	606:733	METHODS The 162 eligible patients with gynecologic cancers who were treated with either dalteparin (n=60) or rivaroxaban (n=102) were reviewed.					
31789000	10	23	theme	urinary	1530:1536	arg1	%					1549:1549	44.4%	1545:1549	44.4%	1545:1549	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	10	23	theme	urinary	1530:1536	arg1	tract					1538:1542	the urinary tract	1526:1542	the urinary tract (44.4%)	1526:1550	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	11	24	theme	dalteparin	1715:1724	arg1	groups					1726:1731	the rivaroxaban and dalteparin groups	1695:1731	groups	1726:1731	CONCLUSION In this study, although there were no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups, rivaroxaban use was associated with a higher rate of clinically relevant bleeding than dalteparin.					
31789000	11	25	theme	bleeding	1807:1814	arg1	rate					1779:1782	a higher rate	1770:1782	a higher rate of clinically relevant bleeding than dalteparin	1770:1830	CONCLUSION In this study, although there were no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups, rivaroxaban use was associated with a higher rate of clinically relevant bleeding than dalteparin.					
31789000	8	26	theme	significant	1235:1245	arg1	difference					1247:1256	no significant difference	1232:1256	no significant difference in the number of composite events and clinically relevant bleeding between the groups	1232:1342	Multivariate analysis using the Cox proportional hazards model also showed no significant difference in the number of composite events and clinically relevant bleeding between the groups.					
31789000	5	27	theme	bleeding	842:849	arg1	events					851:856	clinically relevant bleeding events	822:856	clinically relevant bleeding events	822:856	The primary outcome was a composite event, which included recurrence or clinically relevant bleeding events during the therapeutic period.					
31789000	8	28	theme	hazards	1206:1212	arg1	model					1214:1218	the Cox proportional hazards model	1185:1218	the Cox proportional hazards model	1185:1218	Multivariate analysis using the Cox proportional hazards model also showed no significant difference in the number of composite events and clinically relevant bleeding between the groups.					
31789000	7	29	theme	therapeutic	996:1006	arg1	period					1008:1013	the therapeutic period	992:1013	the therapeutic period	992:1013	RESULTS During the therapeutic period, there were no significant differences between the groups in the proportion of composite events, recurrence, or clinically relevant bleeding.					
31789000	8	30	theme	Multivariate	1157:1168	arg1	analysis					1170:1177	Multivariate analysis	1157:1177	Multivariate analysis using the Cox proportional hazards model	1157:1218	Multivariate analysis using the Cox proportional hazards model also showed no significant difference in the number of composite events and clinically relevant bleeding between the groups.					
31789000	11	31	from	differences	1648:1658	arg1	effectiveness					1663:1675	effectiveness	1663:1675	effectiveness	1663:1675	CONCLUSION In this study, although there were no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups, rivaroxaban use was associated with a higher rate of clinically relevant bleeding than dalteparin.					
31789000	11	31	from	differences	1648:1658	arg1	safety					1680:1685	safety	1680:1685	safety	1680:1685	CONCLUSION In this study, although there were no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups, rivaroxaban use was associated with a higher rate of clinically relevant bleeding than dalteparin.					
31789000	0	32	with	patients	98:105	arg1	cancers					124:130	gynecologic cancers	112:130	gynecologic cancers	112:130	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	10	33	from	site	1573:1576	arg1	common					1516:1521	common	1516:1521	common	1516:1521	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	4	34	with	patients	631:638	arg1	cancers					657:663	gynecologic cancers	645:663	gynecologic cancers	645:663	METHODS The 162 eligible patients with gynecologic cancers who were treated with either dalteparin (n=60) or rivaroxaban (n=102) were reviewed.					
31789000	3	35	with	patients	480:487	arg1	cancers					516:522	primary gynecological cancers	494:522	primary gynecological cancers	494:522	We compared the outcomes of patients with primary gynecological cancers who were treated for cancer-associated VTE with either rivaroxaban or dalteparin.					
31789000	1	36	dep	randomized	148:157	arg1	controlled					160:169	controlled	160:169	controlled	160:169	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	3	37	theme	primary	494:500	arg1	cancers					516:522	primary gynecological cancers	494:522	primary gynecological cancers	494:522	We compared the outcomes of patients with primary gynecological cancers who were treated for cancer-associated VTE with either rivaroxaban or dalteparin.					
31789000	0	38	theme	rivaroxaban	14:24	arg1	Comparison					0:9	Comparison	0:9	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.	0:131	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	10	39	from	common	1516:1521	arg1	%					1549:1549	44.4%	1545:1549	44.4%	1545:1549	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	10	39	from	common	1516:1521	arg1	group					1491:1495	the dalteparin group	1476:1495	the dalteparin group	1476:1495	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	10	39	from	common	1516:1521	arg1	tract					1538:1542	the urinary tract	1526:1542	the urinary tract (44.4%)	1526:1550	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	0	40	from	thromboembolism	79:93	arg1	patients					98:105	patients	98:105	patients with gynecologic cancers	98:130	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	10	41	from	group	1491:1495	arg1	common					1516:1521	common	1516:1521	common	1516:1521	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	3	42	theme	gynecological	502:514	arg1	cancers					516:522	primary gynecological cancers	494:522	primary gynecological cancers	494:522	We compared the outcomes of patients with primary gynecological cancers who were treated for cancer-associated VTE with either rivaroxaban or dalteparin.					
31789000	8	43	theme	composite	1275:1283	arg1	events					1285:1290	composite events	1275:1290	composite events	1275:1290	Multivariate analysis using the Cox proportional hazards model also showed no significant difference in the number of composite events and clinically relevant bleeding between the groups.					
31789000	2	44	theme	populations	439:449	arg1	populations					439:449	the study populations	429:449	the study populations	429:449	However, gynecologic cancers accounted for approximately 10% of the study populations.					
31789000	2	44	theme	populations	439:449	arg1	%					424:424	approximately 10%	408:424	approximately 10% of the study populations	408:449	However, gynecologic cancers accounted for approximately 10% of the study populations.					
31789000	1	45	theme	direct	223:228	arg1	anticoagulants					235:248	direct oral anticoagulants	223:248	direct oral anticoagulants	223:248	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	0	46	theme	dalteparin	30:39	arg1	Comparison					0:9	Comparison	0:9	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.	0:131	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	5	47	theme	primary	754:760	arg1	outcome					762:768	The primary outcome	750:768	The primary outcome	750:768	The primary outcome was a composite event, which included recurrence or clinically relevant bleeding events during the therapeutic period.					
31789000	5	47	theme	primary	754:760	arg1	event					786:790	a composite event	774:790	a composite event	774:790	The primary outcome was a composite event, which included recurrence or clinically relevant bleeding events during the therapeutic period.					
31789000	1	48	theme	oral	230:233	arg1	anticoagulants					235:248	direct oral anticoagulants	223:248	direct oral anticoagulants	223:248	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	0	49	theme	long-term	49:57	arg1	treatment					59:67	the long-term treatment	45:67	the long-term treatment of venous thromboembolism in patients with gynecologic cancers	45:130	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	7	50	theme	composite	1094:1102	arg1	events					1104:1109	composite events	1094:1109	composite events	1094:1109	RESULTS During the therapeutic period, there were no significant differences between the groups in the proportion of composite events, recurrence, or clinically relevant bleeding.					
31789000	0	51	from	treatment	59:67	arg1	patients					98:105	patients	98:105	patients with gynecologic cancers	98:130	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	6	52	theme	relevant	936:943	arg1	events					954:959	clinically relevant bleeding events	925:959	clinically relevant bleeding events	925:959	Secondary outcomes were recurrence, clinically relevant bleeding events, and mortality.					
31789000	2	53	theme	gynecologic	374:384	arg1	cancers					386:392	gynecologic cancers	374:392	gynecologic cancers	374:392	However, gynecologic cancers accounted for approximately 10% of the study populations.					
31789000	5	54	theme	relevant	833:840	arg1	events					851:856	clinically relevant bleeding events	822:856	clinically relevant bleeding events	822:856	The primary outcome was a composite event, which included recurrence or clinically relevant bleeding events during the therapeutic period.					
31789000	10	55	from	tract	1538:1542	arg1	common					1516:1521	common	1516:1521	common	1516:1521	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	7	56	theme	events	1104:1109	arg1	proportion					1080:1089	the proportion	1076:1089	the proportion of composite events	1076:1109	RESULTS During the therapeutic period, there were no significant differences between the groups in the proportion of composite events, recurrence, or clinically relevant bleeding.					
31789000	6	57	theme	Secondary	889:897	arg1	outcomes					899:906	Secondary outcomes	889:906	Secondary outcomes	889:906	Secondary outcomes were recurrence, clinically relevant bleeding events, and mortality.					
31789000	10	58	theme	dalteparin	1480:1489	arg1	group					1491:1495	the dalteparin group	1476:1495	the dalteparin group	1476:1495	In the dalteparin group, bleeding was most common in the urinary tract (44.4%) and at the injection site (22.2%).					
31789000	11	59	theme	significant	1636:1646	arg1	differences					1648:1658	no significant differences	1633:1658	no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups	1633:1731	CONCLUSION In this study, although there were no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups, rivaroxaban use was associated with a higher rate of clinically relevant bleeding than dalteparin.					
31789000	11	60	theme	higher	1772:1777	arg1	rate					1779:1782	a higher rate	1770:1782	a higher rate of clinically relevant bleeding than dalteparin	1770:1830	CONCLUSION In this study, although there were no significant differences in effectiveness or safety between the rivaroxaban and dalteparin groups, rivaroxaban use was associated with a higher rate of clinically relevant bleeding than dalteparin.					
31789000	0	61	from	patients	98:105	arg1	treatment					59:67	the long-term treatment	45:67	the long-term treatment of venous thromboembolism in patients with gynecologic cancers	45:130	Comparison of rivaroxaban and dalteparin for the long-term treatment of venous thromboembolism in patients with gynecologic cancers.					
31789000	12	62	theme	gynecologic	1901:1911	arg1	VTE					1931:1933	gynecologic cancer-associated VTE	1901:1933	gynecologic cancer-associated VTE	1901:1933	Therefore, caution should be taken when prescribing rivaroxaban for gynecologic cancer-associated VTE and bleeding events should be carefully monitored.					
31789000	8	63	theme	proportional	1193:1204	arg1	model					1214:1218	the Cox proportional hazards model	1185:1218	the Cox proportional hazards model	1185:1218	Multivariate analysis using the Cox proportional hazards model also showed no significant difference in the number of composite events and clinically relevant bleeding between the groups.					
31789000	2	64	theme	study	433:437	arg1	populations					439:449	the study populations	429:449	the study populations	429:449	However, gynecologic cancers accounted for approximately 10% of the study populations.					
31789000	3	65	theme	patients	480:487	arg1	outcomes					468:475	the outcomes	464:475	the outcomes of patients with primary gynecological cancers who were treated for cancer-associated VTE with either rivaroxaban or dalteparin	464:603	We compared the outcomes of patients with primary gynecological cancers who were treated for cancer-associated VTE with either rivaroxaban or dalteparin.					
31789000	5	66	theme	therapeutic	869:879	arg1	period					881:886	the therapeutic period	865:886	the therapeutic period	865:886	The primary outcome was a composite event, which included recurrence or clinically relevant bleeding events during the therapeutic period.					
31789000	1	67	theme	randomized	148:157	arg1	studies					171:177	Two randomized, controlled studies	144:177	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE)	133:331	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	1	68	theme	low-molecular	253:265	arg1	heparin					274:280	low-molecular weight heparin	253:280	low-molecular weight heparin	253:280	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
31789000	4	69	theme	gynecologic	645:655	arg1	cancers					657:663	gynecologic cancers	645:663	gynecologic cancers	645:663	METHODS The 162 eligible patients with gynecologic cancers who were treated with either dalteparin (n=60) or rivaroxaban (n=102) were reviewed.					
31789000	9	70	theme	patients	1380:1387	arg1	%					1375:1375	44.0%	1371:1375	44.0% of patients	1371:1387	In the rivaroxaban group, 44.0% of patients experienced gastrointestinal bleeding and 24.0% experienced urinary tract bleeding.					
31789000	9	70	theme	patients	1380:1387	arg1	patients					1380:1387	patients	1380:1387	patients	1380:1387	In the rivaroxaban group, 44.0% of patients experienced gastrointestinal bleeding and 24.0% experienced urinary tract bleeding.					
31789000	7	71	dep	RESULTS	977:983	arg1	were					1022:1025	were	1022:1025	were	1022:1025	RESULTS During the therapeutic period, there were no significant differences between the groups in the proportion of composite events, recurrence, or clinically relevant bleeding.					
31789000	1	72	theme	weight	267:272	arg1	heparin					274:280	low-molecular weight heparin	253:280	low-molecular weight heparin	253:280	OBJECTIVES Two randomized, controlled studies comparing outcomes in patients treated with direct oral anticoagulants or low-molecular weight heparin for cancer-associated venous thromboembolism (VTE) have previously been performed.					
30927523	0	0	from	Sorbent	4:10	arg1	Basis					19:23	the Basis	15:23	the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids	15:137	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.					
30927523	2	1	theme	developed	372:380	arg1	sorbent					382:388	the developed sorbent	368:388	the developed sorbent	368:388	Experimentally confirmed sorption capacity for the developed sorbent was at least 400 ng of endotoxin per ml sorbent.					
30927523	0	2	theme	Lipopolysaccharide	85:102	arg1	Removal					64:70	Removal	64:70	Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids	64:137	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.					
30927523	0	3	theme	Bacterial	75:83	arg1	Lipopolysaccharide					85:102	Bacterial Lipopolysaccharide	75:102	Bacterial Lipopolysaccharide (Endotoxin)	75:114	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.					
30927523	0	3	theme	Bacterial	75:83	arg1	Endotoxin					105:113	Endotoxin	105:113	Endotoxin	105:113	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.					
30927523	2	4	theme	sorption	346:353	arg1	capacity					355:362	Experimentally confirmed sorption capacity	321:362	Experimentally confirmed sorption capacity for the developed sorbent	321:388	Experimentally confirmed sorption capacity for the developed sorbent was at least 400 ng of endotoxin per ml sorbent.					
30927523	2	5	theme	ml	427:428	arg1	sorbent					430:436	ml sorbent	427:436	ml sorbent	427:436	Experimentally confirmed sorption capacity for the developed sorbent was at least 400 ng of endotoxin per ml sorbent.					
30927523	3	6	theme	new	443:445	arg1	compatible					458:467	compatible	458:467	compatible	458:467	The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.					
30927523	3	6	theme	new	443:445	arg1	sorbent					447:453	The new sorbent	439:453	The new sorbent	439:453	The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.					
30927523	3	7	theme	extracorporeal	527:540	arg1	therapy					542:548	extracorporeal therapy	527:548	extracorporeal therapy	527:548	The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.					
30927523	2	8	theme	confirmed	336:344	arg1	capacity					355:362	Experimentally confirmed sorption capacity	321:362	Experimentally confirmed sorption capacity for the developed sorbent	321:388	Experimentally confirmed sorption capacity for the developed sorbent was at least 400 ng of endotoxin per ml sorbent.					
30927523	1	9	theme	first	168:172	arg1	time					174:177	the first time that immobilized lysozyme can efficiently remove Escherichia coli and Pseudomonas aeruginosa lipopolysaccharides (endotoxins) from solutions	164:318	the first time that immobilized lysozyme can efficiently remove Escherichia coli and Pseudomonas aeruginosa lipopolysaccharides (endotoxins) from solutions	164:318	It was demonstrated for the first time that immobilized lysozyme can efficiently remove Escherichia coli and Pseudomonas aeruginosa lipopolysaccharides (endotoxins) from solutions.					
30927523	0	10	theme	New	0:2	arg1	Sorbent					4:10	New Sorbent	0:10	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.	0:138	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.					
30927523	3	11	theme	sepsis	570:575	arg1	treatment					557:565	the treatment	553:565	the treatment of sepsis	553:575	The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.					
30927523	3	12	with	compatible	458:467	arg1	blood					490:494	the whole human blood	474:494	the whole human blood	474:494	The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.					
30927523	1	13	theme	immobilized	184:194	arg1	lysozyme					196:203	immobilized lysozyme	184:203	immobilized lysozyme	184:203	It was demonstrated for the first time that immobilized lysozyme can efficiently remove Escherichia coli and Pseudomonas aeruginosa lipopolysaccharides (endotoxins) from solutions.					
30927523	1	14	dep	coli	240:243	arg1	lipopolysaccharides					272:290	lipopolysaccharides	272:290	lipopolysaccharides	272:290	It was demonstrated for the first time that immobilized lysozyme can efficiently remove Escherichia coli and Pseudomonas aeruginosa lipopolysaccharides (endotoxins) from solutions.					
30927523	0	15	theme	Biological	121:130	arg1	Fluids					132:137	Biological Fluids	121:137	Biological Fluids	121:137	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.					
30927523	2	16	theme	endotoxin	413:421	arg1	ng					407:408	at least 400 ng	394:408	at least 400 ng of endotoxin per ml sorbent	394:436	Experimentally confirmed sorption capacity for the developed sorbent was at least 400 ng of endotoxin per ml sorbent.					
30927523	0	17	theme	Immobilized	39:49	arg1	Lysozyme					51:58	Covalently Immobilized Lysozyme	28:58	Covalently Immobilized Lysozyme	28:58	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.					
30927523	3	18	theme	whole	478:482	arg1	blood					490:494	the whole human blood	474:494	the whole human blood	474:494	The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.					
30927523	0	19	from	Fluids	132:137	arg1	Removal					64:70	Removal	64:70	Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids	64:137	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.					
30927523	3	20	theme	human	484:488	arg1	blood					490:494	the whole human blood	474:494	the whole human blood	474:494	The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.					
30927523	3	21	used	used	519:522	arg2	compatible					458:467	compatible	458:467	compatible	458:467	The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.					
30927523	3	21	used	used	519:522	arg2	sorbent					447:453	The new sorbent	439:453	The new sorbent	439:453	The new sorbent is compatible with the whole human blood and can be potentially used in extracorporeal therapy in the treatment of sepsis.					
30927523	0	22	theme	Lysozyme	51:58	arg1	Basis					19:23	the Basis	15:23	the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids	15:137	New Sorbent on the Basis of Covalently Immobilized Lysozyme for Removal of Bacterial Lipopolysaccharide (Endotoxin) from Biological Fluids.					
29653498	5	0	theme	PLGA/CNC	749:756	arg1	membranes					768:776	NT-loaded PLGA/CNC nanofiber membranes	739:776	NT-loaded PLGA/CNC nanofiber membranes	739:776	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	6	1	theme	composite	919:927	arg1	membranes					939:947	NT-loaded PLGA/CNC composite nanofiber membranes	900:947	NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks	900:959	The results showed that NT could be sustained released from NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks.					
29653498	10	2	theme	composite	1391:1399	arg1	membranes					1411:1419	NT-loaded PLGA/CNC composite nanofiber membranes	1372:1419	NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT	1372:1448	These results suggest that NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT should effectively promote tissue regeneration for the treatment of DFUs.					
29653498	5	3	theme	nanofiber	758:766	arg1	membranes					768:776	NT-loaded PLGA/CNC nanofiber membranes	739:776	NT-loaded PLGA/CNC nanofiber membranes	739:776	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	6	4	theme	PLGA/CNC	910:917	arg1	membranes					939:947	NT-loaded PLGA/CNC composite nanofiber membranes	900:947	NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks	900:959	The results showed that NT could be sustained released from NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks.					
29653498	10	5	theme	DFUs	1518:1521	arg1	treatment					1505:1513	the treatment	1501:1513	the treatment of DFUs	1501:1521	These results suggest that NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT should effectively promote tissue regeneration for the treatment of DFUs.					
29653498	7	6	theme	composite	981:989	arg1	membranes					1001:1009	NT-loaded PLGA/CNC composite nanofiber membranes	962:1009	NT-loaded PLGA/CNC composite nanofiber membranes	962:1009	NT-loaded PLGA/CNC composite nanofiber membranes induced more rapid healing than other control groups.					
29653498	8	7	theme	area	1180:1183	arg1	scores					1101:1106	the histological scores	1084:1106	the histological scores of the epidermal and dermal regeneration	1084:1147	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	8	7	theme	area	1180:1183	arg1	ratios					1157:1162	the ratios	1153:1162	the ratios of the fibrotic area to the whole area	1153:1201	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	6	8	theme	NT-loaded	900:908	arg1	membranes					939:947	NT-loaded PLGA/CNC composite nanofiber membranes	900:947	NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks	900:959	The results showed that NT could be sustained released from NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks.					
29653498	8	9	theme	NT	1071:1072	arg1	exposure					1074:1081	NT exposure	1071:1081	NT exposure	1071:1081	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	7	10	theme	nanofiber	991:999	arg1	membranes					1001:1009	NT-loaded PLGA/CNC composite nanofiber membranes	962:1009	NT-loaded PLGA/CNC composite nanofiber membranes	962:1009	NT-loaded PLGA/CNC composite nanofiber membranes induced more rapid healing than other control groups.					
29653498	8	11	theme	fibrotic	1171:1178	arg1	area					1180:1183	the fibrotic area	1167:1183	the fibrotic area	1167:1183	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	3	12	dep	polylactide-polyglycolide	350:374	arg1	membranes					438:446	nanofiber membranes	428:446	nanofiber membranes	428:446	As we demonstrated previously, polylactide-polyglycolide (PLGA) and cellulose nanocrystals (CNCs) (PLGA/CNC) nanofiber membranes show good cytocompatibility and facilitate fibroblast adhesion, spreading and proliferation.					
29653498	7	13	theme	NT-loaded	962:970	arg1	membranes					1001:1009	NT-loaded PLGA/CNC composite nanofiber membranes	962:1009	NT-loaded PLGA/CNC composite nanofiber membranes	962:1009	NT-loaded PLGA/CNC composite nanofiber membranes induced more rapid healing than other control groups.					
29653498	4	14	theme	novel	574:578	arg1	membranes					560:568	PLGA/CNC nanofiber membranes	541:568	PLGA/CNC nanofiber membranes	541:568	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	4	14	theme	novel	574:578	arg1	materials					580:588	novel materials	574:588	novel materials that have not been used previously as NT carriers in diabetic wounds	574:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	4	14	theme	novel	574:578	arg1	carriers					631:638	NT carriers	628:638	NT carriers in diabetic wounds	628:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	5	15	theme	possible	716:723	arg1	mechanisms					725:734	possible mechanisms	716:734	possible mechanisms	716:734	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	7	16	theme	PLGA/CNC	972:979	arg1	membranes					1001:1009	NT-loaded PLGA/CNC composite nanofiber membranes	962:1009	NT-loaded PLGA/CNC composite nanofiber membranes	962:1009	NT-loaded PLGA/CNC composite nanofiber membranes induced more rapid healing than other control groups.					
29653498	1	17	theme	Diabetic	93:100	arg1	threat					127:132	a threat	125:132	a threat to human health	125:148	Diabetic foot ulcers (DFUs) are a threat to human health and can lead to amputation and even death.					
29653498	1	17	theme	Diabetic	93:100	arg1	DFUs					115:118	DFUs	115:118	DFUs	115:118	Diabetic foot ulcers (DFUs) are a threat to human health and can lead to amputation and even death.					
29653498	1	17	theme	Diabetic	93:100	arg1	ulcers					107:112	Diabetic foot ulcers	93:112	Diabetic foot ulcers (DFUs)	93:119	Diabetic foot ulcers (DFUs) are a threat to human health and can lead to amputation and even death.					
29653498	5	18	theme	skin	796:799	arg1	wounds					801:806	full-thickness skin wounds	781:806	full-thickness skin wounds in spontaneously diabetic mice	781:837	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	5	19	from	wounds	801:806	arg1	mice					834:837	spontaneously diabetic mice	811:837	spontaneously diabetic mice	811:837	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	10	20	theme	tissue	1477:1482	arg1	regeneration					1484:1495	tissue regeneration	1477:1495	tissue regeneration	1477:1495	These results suggest that NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT should effectively promote tissue regeneration for the treatment of DFUs.					
29653498	1	21	theme	foot	102:105	arg1	threat					127:132	a threat	125:132	a threat to human health	125:148	Diabetic foot ulcers (DFUs) are a threat to human health and can lead to amputation and even death.					
29653498	1	21	theme	foot	102:105	arg1	DFUs					115:118	DFUs	115:118	DFUs	115:118	Diabetic foot ulcers (DFUs) are a threat to human health and can lead to amputation and even death.					
29653498	1	21	theme	foot	102:105	arg1	ulcers					107:112	Diabetic foot ulcers	93:112	Diabetic foot ulcers (DFUs)	93:119	Diabetic foot ulcers (DFUs) are a threat to human health and can lead to amputation and even death.					
29653498	0	22	theme	PLGA/CNC	19:26	arg1	membranes					48:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes	0:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes	0:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes accelerate diabetic wound healing.					
29653498	8	23	theme	whole	1192:1196	arg1	area					1198:1201	the whole area	1188:1201	the whole area	1188:1201	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	8	24	theme	histological	1088:1099	arg1	scores					1101:1106	the histological scores	1084:1106	the histological scores of the epidermal and dermal regeneration	1084:1147	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	9	25	theme	NT-loaded	1219:1227	arg1	membranes					1258:1266	NT-loaded PLGA/CNC composite nanofiber membranes	1219:1266	NT-loaded PLGA/CNC composite nanofiber membranes	1219:1266	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	4	26	from	carriers	631:638	arg1	wounds					652:657	diabetic wounds	643:657	diabetic wounds	643:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	3	27	theme	cellulose	387:395	arg1	CNCs					411:414	CNCs	411:414	CNCs	411:414	As we demonstrated previously, polylactide-polyglycolide (PLGA) and cellulose nanocrystals (CNCs) (PLGA/CNC) nanofiber membranes show good cytocompatibility and facilitate fibroblast adhesion, spreading and proliferation.					
29653498	3	27	theme	cellulose	387:395	arg1	PLGA/CNC					418:425	PLGA/CNC	418:425	PLGA/CNC	418:425	As we demonstrated previously, polylactide-polyglycolide (PLGA) and cellulose nanocrystals (CNCs) (PLGA/CNC) nanofiber membranes show good cytocompatibility and facilitate fibroblast adhesion, spreading and proliferation.					
29653498	3	27	theme	cellulose	387:395	arg1	nanocrystals					397:408	cellulose nanocrystals	387:408	cellulose nanocrystals (CNCs) (PLGA/CNC)	387:426	As we demonstrated previously, polylactide-polyglycolide (PLGA) and cellulose nanocrystals (CNCs) (PLGA/CNC) nanofiber membranes show good cytocompatibility and facilitate fibroblast adhesion, spreading and proliferation.					
29653498	0	28	theme	Neurotensin-loaded	0:17	arg1	membranes					48:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes	0:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes	0:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes accelerate diabetic wound healing.					
29653498	9	29	theme	PLGA/CNC	1229:1236	arg1	membranes					1258:1266	NT-loaded PLGA/CNC composite nanofiber membranes	1219:1266	NT-loaded PLGA/CNC composite nanofiber membranes	1219:1266	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	4	30	theme	diabetic	643:650	arg1	wounds					652:657	diabetic wounds	643:657	diabetic wounds	643:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	0	31	theme	nanofiber	38:46	arg1	membranes					48:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes	0:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes	0:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes accelerate diabetic wound healing.					
29653498	9	32	theme	composite	1238:1246	arg1	membranes					1258:1266	NT-loaded PLGA/CNC composite nanofiber membranes	1219:1266	NT-loaded PLGA/CNC composite nanofiber membranes	1219:1266	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	10	33	theme	NT	1447:1448	arg1	delivery					1435:1442	sustained delivery	1425:1442	sustained delivery of NT	1425:1448	These results suggest that NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT should effectively promote tissue regeneration for the treatment of DFUs.					
29653498	1	34	theme	even	181:184	arg1	death					186:190	even death	181:190	even death	181:190	Diabetic foot ulcers (DFUs) are a threat to human health and can lead to amputation and even death.					
29653498	0	35	theme	composite	28:36	arg1	membranes					48:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes	0:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes	0:56	Neurotensin-loaded PLGA/CNC composite nanofiber membranes accelerate diabetic wound healing.					
29653498	4	36	theme	nanofiber	550:558	arg1	membranes					560:568	PLGA/CNC nanofiber membranes	541:568	PLGA/CNC nanofiber membranes	541:568	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	4	36	theme	nanofiber	550:558	arg1	materials					580:588	novel materials	574:588	novel materials that have not been used previously as NT carriers in diabetic wounds	574:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	2	37	theme	wound	249:253	arg1	healing					255:261	wound healing	249:261	wound healing	249:261	Recently neurotensin (NT), an inflammatory modulator in wound healing, was found to be beneficial for diabetic wound healing.					
29653498	9	38	theme	nanofiber	1248:1256	arg1	membranes					1258:1266	NT-loaded PLGA/CNC composite nanofiber membranes	1219:1266	NT-loaded PLGA/CNC composite nanofiber membranes	1219:1266	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	2	39	from	modulator	236:244	arg1	healing					255:261	wound healing	249:261	wound healing	249:261	Recently neurotensin (NT), an inflammatory modulator in wound healing, was found to be beneficial for diabetic wound healing.					
29653498	5	40	theme	membranes	768:776	arg1	efficacy					703:710	therapeutic efficacy	691:710	therapeutic efficacy	691:710	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	5	40	theme	membranes	768:776	arg1	mechanisms					725:734	possible mechanisms	716:734	possible mechanisms	716:734	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	4	41	theme	PLGA/CNC	541:548	arg1	membranes					560:568	PLGA/CNC nanofiber membranes	541:568	PLGA/CNC nanofiber membranes	541:568	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	4	41	theme	PLGA/CNC	541:548	arg1	materials					580:588	novel materials	574:588	novel materials that have not been used previously as NT carriers in diabetic wounds	574:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	8	42	theme	epidermal	1115:1123	arg1	regeneration					1136:1147	the epidermal and dermal regeneration	1111:1147	the epidermal and dermal regeneration	1111:1147	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	5	43	from	mechanisms	725:734	arg1	wounds					801:806	full-thickness skin wounds	781:806	full-thickness skin wounds in spontaneously diabetic mice	781:837	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	10	44	theme	NT-loaded	1372:1380	arg1	membranes					1411:1419	NT-loaded PLGA/CNC composite nanofiber membranes	1372:1419	NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT	1372:1448	These results suggest that NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT should effectively promote tissue regeneration for the treatment of DFUs.					
29653498	4	45	theme	NT	628:629	arg1	materials					580:588	novel materials	574:588	novel materials that have not been used previously as NT carriers in diabetic wounds	574:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	4	45	theme	NT	628:629	arg1	carriers					631:638	NT carriers	628:638	NT carriers in diabetic wounds	628:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	9	46	dep	cytokines	1319:1327	arg1	IL-1β					1329:1333	IL-1β	1329:1333	IL-1β	1329:1333	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	9	46	dep	cytokines	1319:1327	arg1	cytokines					1319:1327	the inflammatory cytokines	1302:1327	the inflammatory cytokines IL-1β and IL-6	1302:1342	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	9	46	dep	cytokines	1319:1327	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	5	47	theme	therapeutic	691:701	arg1	efficacy					703:710	therapeutic efficacy	691:710	therapeutic efficacy	691:710	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	5	48	from	efficacy	703:710	arg1	wounds					801:806	full-thickness skin wounds	781:806	full-thickness skin wounds in spontaneously diabetic mice	781:837	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	7	49	theme	other	1043:1047	arg1	groups					1057:1062	other control groups	1043:1062	other control groups	1043:1062	NT-loaded PLGA/CNC composite nanofiber membranes induced more rapid healing than other control groups.					
29653498	0	50	theme	wound	78:82	arg1	healing					84:90	diabetic wound healing	69:90	diabetic wound healing	69:90	Neurotensin-loaded PLGA/CNC composite nanofiber membranes accelerate diabetic wound healing.					
29653498	8	51	theme	dermal	1129:1134	arg1	regeneration					1136:1147	the epidermal and dermal regeneration	1111:1147	the epidermal and dermal regeneration	1111:1147	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	5	52	theme	NT-loaded	739:747	arg1	membranes					768:776	NT-loaded PLGA/CNC nanofiber membranes	739:776	NT-loaded PLGA/CNC nanofiber membranes	739:776	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	7	53	theme	control	1049:1055	arg1	groups					1057:1062	other control groups	1043:1062	other control groups	1043:1062	NT-loaded PLGA/CNC composite nanofiber membranes induced more rapid healing than other control groups.					
29653498	2	54	theme	wound	304:308	arg1	healing					310:316	diabetic wound healing	295:316	diabetic wound healing	295:316	Recently neurotensin (NT), an inflammatory modulator in wound healing, was found to be beneficial for diabetic wound healing.					
29653498	0	55	theme	diabetic	69:76	arg1	healing					84:90	diabetic wound healing	69:90	diabetic wound healing	69:90	Neurotensin-loaded PLGA/CNC composite nanofiber membranes accelerate diabetic wound healing.					
29653498	9	56	theme	inflammatory	1306:1317	arg1	IL-1β					1329:1333	IL-1β	1329:1333	IL-1β	1329:1333	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	9	56	theme	inflammatory	1306:1317	arg1	cytokines					1319:1327	the inflammatory cytokines	1302:1327	the inflammatory cytokines IL-1β and IL-6	1302:1342	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	9	56	theme	inflammatory	1306:1317	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	9	57	theme	cytokines	1319:1327	arg1	expressions					1287:1297	the expressions	1283:1297	the expressions of the inflammatory cytokines IL-1β and IL-6	1283:1342	NT-loaded PLGA/CNC composite nanofiber membranes also decreased the expressions of the inflammatory cytokines IL-1β and IL-6.					
29653498	3	58	theme	good	453:456	arg1	cytocompatibility					458:474	good cytocompatibility	453:474	good cytocompatibility	453:474	As we demonstrated previously, polylactide-polyglycolide (PLGA) and cellulose nanocrystals (CNCs) (PLGA/CNC) nanofiber membranes show good cytocompatibility and facilitate fibroblast adhesion, spreading and proliferation.					
29653498	5	59	dep	efficacy	703:710	arg1	the					687:689	the	687:689	the	687:689	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	3	60	theme	nanofiber	428:436	arg1	membranes					438:446	nanofiber membranes	428:446	nanofiber membranes	428:446	As we demonstrated previously, polylactide-polyglycolide (PLGA) and cellulose nanocrystals (CNCs) (PLGA/CNC) nanofiber membranes show good cytocompatibility and facilitate fibroblast adhesion, spreading and proliferation.					
29653498	10	61	theme	nanofiber	1401:1409	arg1	membranes					1411:1419	NT-loaded PLGA/CNC composite nanofiber membranes	1372:1419	NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT	1372:1448	These results suggest that NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT should effectively promote tissue regeneration for the treatment of DFUs.					
29653498	6	62	dep	sustained	876:884	arg1	released					886:893	released	886:893	could be sustained released from NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks	867:959	The results showed that NT could be sustained released from NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks.					
29653498	8	63	theme	regeneration	1136:1147	arg1	scores					1101:1106	the histological scores	1084:1106	the histological scores of the epidermal and dermal regeneration	1084:1147	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	8	63	theme	regeneration	1136:1147	arg1	ratios					1157:1162	the ratios	1153:1162	the ratios of the fibrotic area to the whole area	1153:1201	After NT exposure, the histological scores of the epidermal and dermal regeneration and the ratios of the fibrotic area to the whole area were increased.					
29653498	2	64	theme	inflammatory	223:234	arg1	neurotensin					202:212	neurotensin	202:212	neurotensin (NT)	202:217	Recently neurotensin (NT), an inflammatory modulator in wound healing, was found to be beneficial for diabetic wound healing.					
29653498	2	64	theme	inflammatory	223:234	arg1	modulator					236:244	an inflammatory modulator	220:244	an inflammatory modulator in wound healing	220:261	Recently neurotensin (NT), an inflammatory modulator in wound healing, was found to be beneficial for diabetic wound healing.					
29653498	10	65	theme	PLGA/CNC	1382:1389	arg1	membranes					1411:1419	NT-loaded PLGA/CNC composite nanofiber membranes	1372:1419	NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT	1372:1448	These results suggest that NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT should effectively promote tissue regeneration for the treatment of DFUs.					
29653498	2	66	theme	diabetic	295:302	arg1	healing					310:316	diabetic wound healing	295:316	diabetic wound healing	295:316	Recently neurotensin (NT), an inflammatory modulator in wound healing, was found to be beneficial for diabetic wound healing.					
29653498	7	67	theme	rapid	1024:1028	arg1	healing					1030:1036	more rapid healing	1019:1036	more rapid healing	1019:1036	NT-loaded PLGA/CNC composite nanofiber membranes induced more rapid healing than other control groups.					
29653498	1	68	theme	human	137:141	arg1	health					143:148	human health	137:148	human health	137:148	Diabetic foot ulcers (DFUs) are a threat to human health and can lead to amputation and even death.					
29653498	10	69	theme	sustained	1425:1433	arg1	delivery					1435:1442	sustained delivery	1425:1442	sustained delivery of NT	1425:1448	These results suggest that NT-loaded PLGA/CNC composite nanofiber membranes for sustained delivery of NT should effectively promote tissue regeneration for the treatment of DFUs.					
29653498	5	70	theme	full-thickness	781:794	arg1	wounds					801:806	full-thickness skin wounds	781:806	full-thickness skin wounds in spontaneously diabetic mice	781:837	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	6	71	theme	nanofiber	929:937	arg1	membranes					939:947	NT-loaded PLGA/CNC composite nanofiber membranes	900:947	NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks	900:959	The results showed that NT could be sustained released from NT-loaded PLGA/CNC composite nanofiber membranes for 2 weeks.					
29653498	5	72	theme	diabetic	825:832	arg1	mice					834:837	spontaneously diabetic mice	811:837	spontaneously diabetic mice	811:837	This study aims to explore the therapeutic efficacy and possible mechanisms of NT-loaded PLGA/CNC nanofiber membranes in full-thickness skin wounds in spontaneously diabetic mice.					
29653498	4	73	used	used	609:612	arg2	membranes					560:568	PLGA/CNC nanofiber membranes	541:568	PLGA/CNC nanofiber membranes	541:568	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	4	73	used	used	609:612	arg2	materials					580:588	novel materials	574:588	novel materials that have not been used previously as NT carriers in diabetic wounds	574:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	4	73	used	used	609:612	arg2	carriers					631:638	NT carriers	628:638	NT carriers in diabetic wounds	628:657	PLGA/CNC nanofiber membranes are novel materials that have not been used previously as NT carriers in diabetic wounds.					
29653498	3	74	theme	fibroblast	491:500	arg1	adhesion					502:509	fibroblast adhesion	491:509	fibroblast adhesion	491:509	As we demonstrated previously, polylactide-polyglycolide (PLGA) and cellulose nanocrystals (CNCs) (PLGA/CNC) nanofiber membranes show good cytocompatibility and facilitate fibroblast adhesion, spreading and proliferation.					
31887957	5	0	theme	in	718:719	arg1	test					742:745	The in vitro cell viability test	714:745	The in vitro cell viability test (MTT assay)	714:757	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	5	0	theme	in	718:719	arg1	assay					752:756	MTT assay	748:756	MTT assay	748:756	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	1	1	theme	catalyzed	282:290	arg1	reaction					327:334	catalyzed azide-alkyne cycloaddition (CuAAC) reaction	282:334	catalyzed azide-alkyne cycloaddition (CuAAC) reaction	282:334	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	1	2	theme	type	139:142	arg1	nanofibers					182:191	the novel type of guided bone regeneration composite nanofibers	129:191	the novel type of guided bone regeneration composite nanofibers	129:191	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	8	3	theme	bone	1385:1388	arg1	repair					1390:1395	bone repair	1385:1395	bone repair	1385:1395	These results suggested that this novel scaffold provides an interesting option for bone repair and regeneration.					
31887957	0	4	theme	tissue	77:82	arg1	repair					84:89	bone tissue repair	72:89	bone tissue repair	72:89	Electrospun triazole-based chitosan nanofibers as a novel scaffolds for bone tissue repair and regeneration.					
31887957	2	5	used	used	425:428	arg2	bioactivity					353:363	the bioactivity	349:363	the bioactivity of scaffolds the magnesium-doped hydroxyapatite (Mg-HA)	349:419	For improve the bioactivity of scaffolds the magnesium-doped hydroxyapatite (Mg-HA) was used.					
31887957	7	6	theme	Alkaline	1081:1088	arg1	ALP					1103:1105	ALP	1103:1105	ALP	1103:1105	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	7	6	theme	Alkaline	1081:1088	arg1	phosphatase					1090:1100	the Alkaline phosphatase	1077:1100	the Alkaline phosphatase (ALP) activity	1077:1115	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	6	7	theme	CS/PCL/Mg-HA	1037:1048	arg1	scaffolds					1056:1064	CS/PCL/Mg-HA blend scaffolds	1037:1064	CS/PCL/Mg-HA blend scaffolds	1037:1064	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
31887957	7	8	theme	ARS	1137:1139	arg1	staining					1142:1149	Alizarin red S (ARS) staining	1121:1149	Alizarin red S (ARS) staining	1121:1149	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	1	9	theme	cycloaddition	305:317	arg1	reaction					327:334	catalyzed azide-alkyne cycloaddition (CuAAC) reaction	282:334	catalyzed azide-alkyne cycloaddition (CuAAC) reaction	282:334	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	7	10	theme	copolymer	1236:1244	arg1	structure					1219:1227	the chemical structure	1206:1227	the chemical structure of the copolymer	1206:1244	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	5	11	contain	have	796:799	arg1	scaffolds					780:788	fabricated scaffolds	769:788	fabricated scaffolds	769:788	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	5	11	contain	have	796:799	arg2	cytotoxicity					817:828	any significant cytotoxicity	801:828	any significant cytotoxicity	801:828	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	6	12	theme	blend	1050:1054	arg1	scaffolds					1056:1064	CS/PCL/Mg-HA blend scaffolds	1037:1064	CS/PCL/Mg-HA blend scaffolds	1037:1064	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
31887957	4	13	theme	composite	661:669	arg1	nanofibers					671:680	defect-free uniform composite nanofibers	641:680	defect-free uniform composite nanofibers with about 419-495 nm diameter	641:711	The SEM observations show defect-free uniform composite nanofibers with about 419-495 nm diameter.					
31887957	7	14	theme	S	1134:1134	arg1	staining					1142:1149	Alizarin red S (ARS) staining	1121:1149	Alizarin red S (ARS) staining	1121:1149	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	1	15	theme	CuAAC	320:324	arg1	reaction					327:334	catalyzed azide-alkyne cycloaddition (CuAAC) reaction	282:334	catalyzed azide-alkyne cycloaddition (CuAAC) reaction	282:334	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	5	16	theme	fabricated	769:778	arg1	scaffolds					780:788	fabricated scaffolds	769:788	fabricated scaffolds	769:788	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	5	17	theme	MTT	748:750	arg1	test					742:745	The in vitro cell viability test	714:745	The in vitro cell viability test (MTT assay)	714:757	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	5	17	theme	MTT	748:750	arg1	assay					752:756	MTT assay	748:756	MTT assay	748:756	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	1	18	theme	guided	147:152	arg1	regeneration					159:170	guided bone regeneration	147:170	guided bone regeneration	147:170	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	0	19	theme	Electrospun	0:10	arg1	chitosan					27:34	Electrospun triazole-based chitosan	0:34	Electrospun triazole-based chitosan	0:34	Electrospun triazole-based chitosan nanofibers as a novel scaffolds for bone tissue repair and regeneration.					
31887957	1	20	theme	bone	154:157	arg1	regeneration					159:170	guided bone regeneration	147:170	guided bone regeneration	147:170	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	0	21	theme	triazole-based	12:25	arg1	chitosan					27:34	Electrospun triazole-based chitosan	0:34	Electrospun triazole-based chitosan	0:34	Electrospun triazole-based chitosan nanofibers as a novel scaffolds for bone tissue repair and regeneration.					
31887957	7	22	theme	Alizarin	1121:1128	arg1	staining					1142:1149	Alizarin red S (ARS) staining	1121:1149	Alizarin red S (ARS) staining	1121:1149	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	1	23	theme	regeneration	159:170	arg1	type					139:142	the novel type	129:142	the novel type of guided bone regeneration composite nanofibers	129:191	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	1	24	theme	azide-alkyne	292:303	arg1	reaction					327:334	catalyzed azide-alkyne cycloaddition (CuAAC) reaction	282:334	catalyzed azide-alkyne cycloaddition (CuAAC) reaction	282:334	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	1	25	theme	composite	172:180	arg1	nanofibers					182:191	the novel type of guided bone regeneration composite nanofibers	129:191	the novel type of guided bone regeneration composite nanofibers	129:191	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	6	26	theme	CuAAC	914:918	arg1	CS-g-PCL/Mg-HA					930:943	CS-g-PCL/Mg-HA	930:943	CS-g-PCL/Mg-HA	930:943	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
31887957	6	26	theme	CuAAC	914:918	arg1	reaction					920:927	CuAAC reaction	914:927	CuAAC reaction (CS-g-PCL/Mg-HA)	914:944	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
31887957	5	27	dep	in	718:719	arg1	vitro					721:725	vitro	721:725	vitro	721:725	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	3	28	theme	nuclear	526:532	arg1	resonance					543:551	proton nuclear magnetic resonance	519:551	proton nuclear magnetic resonance (1H NMR)	519:560	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	3	28	theme	nuclear	526:532	arg1	NMR					557:559	1H NMR	554:559	1H NMR	554:559	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	8	29	theme	novel	1335:1339	arg1	scaffold					1341:1348	this novel scaffold	1330:1348	this novel scaffold	1330:1348	These results suggested that this novel scaffold provides an interesting option for bone repair and regeneration.					
31887957	2	30	theme	magnesium-doped	382:396	arg1	Mg-HA					414:418	Mg-HA	414:418	Mg-HA	414:418	For improve the bioactivity of scaffolds the magnesium-doped hydroxyapatite (Mg-HA) was used.					
31887957	2	30	theme	magnesium-doped	382:396	arg1	hydroxyapatite					398:411	the magnesium-doped hydroxyapatite	378:411	scaffolds the magnesium-doped hydroxyapatite (Mg-HA)	368:419	For improve the bioactivity of scaffolds the magnesium-doped hydroxyapatite (Mg-HA) was used.					
31887957	3	31	dep	Fourier	467:473	arg1	transform					475:483	transform	475:483	transform infrared spectroscopy (FTIR)	475:512	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	2	32	theme	scaffolds	368:376	arg1	bioactivity					353:363	the bioactivity	349:363	the bioactivity of scaffolds the magnesium-doped hydroxyapatite (Mg-HA)	349:419	For improve the bioactivity of scaffolds the magnesium-doped hydroxyapatite (Mg-HA) was used.					
31887957	5	33	theme	cell	727:730	arg1	test					742:745	The in vitro cell viability test	714:745	The in vitro cell viability test (MTT assay)	714:757	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	5	33	theme	cell	727:730	arg1	assay					752:756	MTT assay	748:756	MTT assay	748:756	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	4	34	theme	defect-free	641:651	arg1	nanofibers					671:680	defect-free uniform composite nanofibers	641:680	defect-free uniform composite nanofibers with about 419-495 nm diameter	641:711	The SEM observations show defect-free uniform composite nanofibers with about 419-495 nm diameter.					
31887957	7	35	theme	red	1130:1132	arg1	staining					1142:1149	Alizarin red S (ARS) staining	1121:1149	Alizarin red S (ARS) staining	1121:1149	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	3	36	theme	magnetic	534:541	arg1	resonance					543:551	proton nuclear magnetic resonance	519:551	proton nuclear magnetic resonance (1H NMR)	519:560	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	3	36	theme	magnetic	534:541	arg1	NMR					557:559	1H NMR	554:559	1H NMR	554:559	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	5	37	theme	significant	805:815	arg1	cytotoxicity					817:828	any significant cytotoxicity	801:828	any significant cytotoxicity	801:828	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	5	38	theme	viability	732:740	arg1	test					742:745	The in vitro cell viability test	714:745	The in vitro cell viability test (MTT assay)	714:757	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	5	38	theme	viability	732:740	arg1	assay					752:756	MTT assay	748:756	MTT assay	748:756	The in vitro cell viability test (MTT assay) show that fabricated scaffolds don't have any significant cytotoxicity.					
31887957	7	39	theme	mineralization	1260:1273	arg1	ability					1275:1281	bone mineralization ability	1255:1281	bone mineralization ability of the scaffolds	1255:1298	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	6	40	theme	antibacterial	958:970	arg1	activity					972:979	higher antibacterial activity	951:979	higher antibacterial activity	951:979	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
31887957	6	41	theme	mechanical	982:991	arg1	properties					993:1002	mechanical properties	982:1002	mechanical properties	982:1002	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
31887957	7	42	theme	phosphatase	1090:1100	arg1	activity					1108:1115	the Alkaline phosphatase (ALP) activity	1077:1115	the Alkaline phosphatase (ALP) activity	1077:1115	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	3	43	theme	proton	519:524	arg1	resonance					543:551	proton nuclear magnetic resonance	519:551	proton nuclear magnetic resonance (1H NMR)	519:560	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	3	43	theme	proton	519:524	arg1	NMR					557:559	1H NMR	554:559	1H NMR	554:559	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	3	44	dep	transform	475:483	arg1	infrared					485:492	infrared	485:492	transform infrared spectroscopy (FTIR)	475:512	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	0	45	theme	novel	52:56	arg1	scaffolds					58:66	a novel scaffolds	50:66	a novel scaffolds for bone tissue repair and regeneration	50:106	Electrospun triazole-based chitosan nanofibers as a novel scaffolds for bone tissue repair and regeneration.					
31887957	7	46	theme	bone	1255:1258	arg1	ability					1275:1281	bone mineralization ability	1255:1281	bone mineralization ability of the scaffolds	1255:1298	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	6	47	theme	MG63	853:856	arg1	cells					858:862	osteoblast-like MG63 cells	837:862	osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA)	837:944	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
31887957	2	48	dep	scaffolds	368:376	arg1	Mg-HA					414:418	Mg-HA	414:418	Mg-HA	414:418	For improve the bioactivity of scaffolds the magnesium-doped hydroxyapatite (Mg-HA) was used.					
31887957	2	48	dep	scaffolds	368:376	arg1	hydroxyapatite					398:411	the magnesium-doped hydroxyapatite	378:411	scaffolds the magnesium-doped hydroxyapatite (Mg-HA)	368:419	For improve the bioactivity of scaffolds the magnesium-doped hydroxyapatite (Mg-HA) was used.					
31887957	1	49	theme	first	117:121	arg1	time					123:126	the first time	113:126	the first time	113:126	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	7	50	theme	triazole	1187:1194	arg1	ring					1196:1199	the triazole ring	1183:1199	the triazole ring	1183:1199	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	4	51	theme	uniform	653:659	arg1	nanofibers					671:680	defect-free uniform composite nanofibers	641:680	defect-free uniform composite nanofibers with about 419-495 nm diameter	641:711	The SEM observations show defect-free uniform composite nanofibers with about 419-495 nm diameter.					
31887957	8	52	theme	interesting	1362:1372	arg1	option					1374:1379	an interesting option	1359:1379	an interesting option for bone repair and regeneration	1359:1412	These results suggested that this novel scaffold provides an interesting option for bone repair and regeneration.					
31887957	7	53	theme	scaffolds	1290:1298	arg1	ability					1275:1281	bone mineralization ability	1255:1281	bone mineralization ability of the scaffolds	1255:1298	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	3	54	theme	Scanning	431:438	arg1	microscopy					449:458	Scanning electron microscopy	431:458	Scanning electron microscopy (SEM)	431:464	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	3	54	theme	Scanning	431:438	arg1	SEM					461:463	SEM	461:463	SEM	461:463	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	7	55	theme	chemical	1210:1217	arg1	structure					1219:1227	the chemical structure	1206:1227	the chemical structure of the copolymer	1206:1244	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	6	56	theme	cell	1009:1012	arg1	attachment					1014:1023	cell attachment	1009:1023	cell attachment	1009:1023	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
31887957	3	57	theme	electron	440:447	arg1	microscopy					449:458	Scanning electron microscopy	431:458	Scanning electron microscopy (SEM)	431:464	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	3	57	theme	electron	440:447	arg1	SEM					461:463	SEM	461:463	SEM	461:463	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	7	58	theme	ring	1196:1199	arg1	introduction					1167:1178	the introduction	1163:1178	the introduction of the triazole ring into the chemical structure of the copolymer	1163:1244	Moreover, the Alkaline phosphatase (ALP) activity and Alizarin red S (ARS) staining showed that the introduction of the triazole ring into the chemical structure of the copolymer enhanced bone mineralization ability of the scaffolds.					
31887957	0	59	theme	bone	72:75	arg1	repair					84:89	bone tissue repair	72:89	bone tissue repair	72:89	Electrospun triazole-based chitosan nanofibers as a novel scaffolds for bone tissue repair and regeneration.					
31887957	3	60	theme	prepared	595:602	arg1	scaffolds					604:612	the prepared scaffolds	591:612	the prepared scaffolds	591:612	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	4	61	theme	SEM	619:621	arg1	observations					623:634	The SEM observations	615:634	The SEM observations	615:634	The SEM observations show defect-free uniform composite nanofibers with about 419-495 nm diameter.					
31887957	6	62	theme	osteoblast-like	837:851	arg1	cells					858:862	osteoblast-like MG63 cells	837:862	osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA)	837:944	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
31887957	4	63	with	nanofibers	671:680	arg1	diameter					704:711	about 419-495 nm diameter	687:711	about 419-495 nm diameter	687:711	The SEM observations show defect-free uniform composite nanofibers with about 419-495 nm diameter.					
31887957	3	64	theme	1H	554:555	arg1	resonance					543:551	proton nuclear magnetic resonance	519:551	proton nuclear magnetic resonance (1H NMR)	519:560	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	3	64	theme	1H	554:555	arg1	NMR					557:559	1H NMR	554:559	1H NMR	554:559	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and proton nuclear magnetic resonance (1H NMR) were applied to characterize the prepared scaffolds.					
31887957	1	65	theme	novel	133:137	arg1	type					139:142	the novel type	129:142	the novel type of guided bone regeneration composite nanofibers	129:191	For the first time, the novel type of guided bone regeneration composite nanofibers were prepared by grafting polycaprolactone (PCL) to chitosan (CS) using the copper (I) - catalyzed azide-alkyne cycloaddition (CuAAC) reaction.					
31887957	6	66	theme	higher	951:956	arg1	activity					972:979	higher antibacterial activity	951:979	higher antibacterial activity	951:979	Also, osteoblast-like MG63 cells cultured on the nanofibers which prepared through CuAAC reaction (CS-g-PCL/Mg-HA) show higher antibacterial activity, mechanical properties, and cell attachment compared to CS/PCL/Mg-HA blend scaffolds.					
29982026	0	0	theme	supercapacitor	77:90	arg1	applications					92:103	supercapacitor applications	77:103	supercapacitor applications	77:103	Hierarchical porous carbon prepared from biomass through a facile method for supercapacitor applications.					
29982026	10	1	theme	general	1176:1182	arg1	way					1184:1186	a general way	1174:1186	a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors	1174:1335	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	5	2	theme	porous	649:654	arg1	carbon					656:661	porous carbon	649:661	porous carbon (OPC)	649:667	This composite was further pyrolyzed under N2 atmosphere to obtain onion derived porous carbon (OPC).					
29982026	5	2	theme	porous	649:654	arg1	OPC					664:666	OPC	664:666	OPC	664:666	This composite was further pyrolyzed under N2 atmosphere to obtain onion derived porous carbon (OPC).					
29982026	6	3	theme	OPC	709:711	arg1	morphology					674:683	morphology	674:683	morphology	674:683	The morphology and porous structure of OPC were characterized by scanning electron microscope and N2 adsorption/desorption.					
29982026	6	3	theme	OPC	709:711	arg1	structure					696:704	porous structure	689:704	porous structure	689:704	The morphology and porous structure of OPC were characterized by scanning electron microscope and N2 adsorption/desorption.					
29982026	7	4	theme	galvanostatic	919:931	arg1	charge-discharge					933:948	galvanostatic charge-discharge	919:948	galvanostatic charge-discharge	919:948	The supercapacitive performances of OPC were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge-discharge.					
29982026	8	5	theme	carbon	1029:1034	arg1	matrix					1036:1041	carbon matrix	1029:1041	carbon matrix	1029:1041	OPC shows high specific surface area with high-volume angstrom-sized pores in carbon matrix.					
29982026	1	6	theme	supercapacitor	166:179	arg1	applications					181:192	supercapacitor applications	166:192	supercapacitor applications	166:192	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	9	7	theme	specific	1108:1115	arg1	capacitance					1117:1127	high specific capacitance	1103:1127	high specific capacitance	1103:1127	When used as supercapacitor electrode materials, OPC shows high specific capacitance and good cycling stability.					
29982026	3	8	from	biomass	413:419	arg1	research					429:436	this research	424:436	this research	424:436	Onion, as the typical biomass in this research, is used as carbon precursor.					
29982026	2	9	theme	carbon	345:350	arg1	preparation					310:320	the preparation	306:320	the preparation of hierarchical porous carbon with high-volume angstrom-sized pores	306:388	In this paper, a facile method is proposed for the preparation of hierarchical porous carbon with high-volume angstrom-sized pores.					
29982026	6	10	dep	morphology	674:683	arg1	The					670:672	The	670:672	The	670:672	The morphology and porous structure of OPC were characterized by scanning electron microscope and N2 adsorption/desorption.					
29982026	8	11	theme	angstrom-sized	1005:1018	arg1	pores					1020:1024	high-volume angstrom-sized pores	993:1024	high-volume angstrom-sized pores in carbon matrix	993:1041	OPC shows high specific surface area with high-volume angstrom-sized pores in carbon matrix.					
29982026	5	12	theme	N2	611:612	arg1	atmosphere					614:623	N2 atmosphere	611:623	N2 atmosphere	611:623	This composite was further pyrolyzed under N2 atmosphere to obtain onion derived porous carbon (OPC).					
29982026	2	13	theme	facile	276:281	arg1	method					283:288	a facile method	274:288	a facile method	274:288	In this paper, a facile method is proposed for the preparation of hierarchical porous carbon with high-volume angstrom-sized pores.					
29982026	7	14	theme	electrochemical	875:889	arg1	spectroscopy					901:912	electrochemical impedance spectroscopy	875:912	electrochemical impedance spectroscopy	875:912	The supercapacitive performances of OPC were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge-discharge.					
29982026	1	15	from	important	153:161	arg1	applications					181:192	supercapacitor applications	166:192	supercapacitor applications	166:192	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	7	16	theme	impedance	891:899	arg1	spectroscopy					901:912	electrochemical impedance spectroscopy	875:912	electrochemical impedance spectroscopy	875:912	The supercapacitive performances of OPC were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge-discharge.					
29982026	8	17	with	area	983:986	arg1	pores					1020:1024	high-volume angstrom-sized pores	993:1024	high-volume angstrom-sized pores in carbon matrix	993:1041	OPC shows high specific surface area with high-volume angstrom-sized pores in carbon matrix.					
29982026	6	18	theme	porous	689:694	arg1	structure					696:704	porous structure	689:704	porous structure	689:704	The morphology and porous structure of OPC were characterized by scanning electron microscope and N2 adsorption/desorption.					
29982026	0	19	theme	porous	13:18	arg1	carbon					20:25	Hierarchical porous carbon	0:25	Hierarchical porous carbon	0:25	Hierarchical porous carbon prepared from biomass through a facile method for supercapacitor applications.					
29982026	1	20	with	carbon	117:122	arg1	pores					144:148	angstrom-sized pores	129:148	angstrom-sized pores	129:148	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	3	21	used	used	442:445	arg2	precursor					457:465	carbon precursor	450:465	carbon precursor	450:465	Onion, as the typical biomass in this research, is used as carbon precursor.					
29982026	3	21	used	used	442:445	arg2	Onion					391:395	Onion	391:395	Onion	391:395	Onion, as the typical biomass in this research, is used as carbon precursor.					
29982026	10	22	theme	supercapacitors	1321:1335	arg1	preparation					1288:1298	preparation	1288:1298	preparation of porous carbon and supercapacitors	1288:1335	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	10	22	theme	supercapacitors	1321:1335	arg1	development					1252:1262	the development	1248:1262	the development of biomass utilization	1248:1285	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	9	23	theme	high	1103:1106	arg1	capacitance					1117:1127	high specific capacitance	1103:1127	high specific capacitance	1103:1127	When used as supercapacitor electrode materials, OPC shows high specific capacitance and good cycling stability.					
29982026	0	24	theme	Hierarchical	0:11	arg1	carbon					20:25	Hierarchical porous carbon	0:25	Hierarchical porous carbon	0:25	Hierarchical porous carbon prepared from biomass through a facile method for supercapacitor applications.					
29982026	4	25	theme	lignin-potassium-salt/cellulose	525:555	arg1	composite					557:565	a water-soluble lignin-potassium-salt/cellulose composite	509:565	a water-soluble lignin-potassium-salt/cellulose composite	509:565	First, onion was etched by KOH to obtain a water-soluble lignin-potassium-salt/cellulose composite.					
29982026	9	26	theme	cycling	1138:1144	arg1	stability					1146:1154	good cycling stability	1133:1154	good cycling stability	1133:1154	When used as supercapacitor electrode materials, OPC shows high specific capacitance and good cycling stability.					
29982026	3	27	theme	carbon	450:455	arg1	Onion					391:395	Onion	391:395	Onion	391:395	Onion, as the typical biomass in this research, is used as carbon precursor.					
29982026	3	27	theme	carbon	450:455	arg1	precursor					457:465	carbon precursor	450:465	carbon precursor	450:465	Onion, as the typical biomass in this research, is used as carbon precursor.					
29982026	4	28	theme	water-soluble	511:523	arg1	composite					557:565	a water-soluble lignin-potassium-salt/cellulose composite	509:565	a water-soluble lignin-potassium-salt/cellulose composite	509:565	First, onion was etched by KOH to obtain a water-soluble lignin-potassium-salt/cellulose composite.					
29982026	6	29	theme	N2	768:769	arg1	adsorption/desorption					771:791	N2 adsorption/desorption	768:791	N2 adsorption/desorption	768:791	The morphology and porous structure of OPC were characterized by scanning electron microscope and N2 adsorption/desorption.					
29982026	7	30	theme	cyclic	855:860	arg1	voltammetry					862:872	cyclic voltammetry	855:872	cyclic voltammetry	855:872	The supercapacitive performances of OPC were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge-discharge.					
29982026	8	31	from	pores	1020:1024	arg1	matrix					1036:1041	carbon matrix	1029:1041	carbon matrix	1029:1041	OPC shows high specific surface area with high-volume angstrom-sized pores in carbon matrix.					
29982026	7	32	theme	supercapacitive	798:812	arg1	performances					814:825	The supercapacitive performances	794:825	The supercapacitive performances of OPC	794:832	The supercapacitive performances of OPC were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge-discharge.					
29982026	1	33	theme	porous	110:115	arg1	important					153:161	important	153:161	important	153:161	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	1	33	theme	porous	110:115	arg1	carbon					117:122	The porous carbon	106:122	The porous carbon with angstrom-sized pores	106:148	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	1	34	theme	high	210:213	arg1	density					220:226	its high pack density	206:226	its high pack density	206:226	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	3	35	theme	typical	405:411	arg1	biomass					413:419	the typical biomass	401:419	the typical biomass in this research	401:436	Onion, as the typical biomass in this research, is used as carbon precursor.					
29982026	9	36	theme	good	1133:1136	arg1	stability					1146:1154	good cycling stability	1133:1154	good cycling stability	1133:1154	When used as supercapacitor electrode materials, OPC shows high specific capacitance and good cycling stability.					
29982026	10	37	theme	porous	1303:1308	arg1	carbon					1310:1315	porous carbon	1303:1315	porous carbon	1303:1315	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	1	38	theme	pack	215:218	arg1	density					220:226	its high pack density	206:226	its high pack density	206:226	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	10	39	theme	from	1213:1216	arg1	biomasses					1218:1226	porous carbon from biomasses	1199:1226	porous carbon from biomasses	1199:1226	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	10	40	theme	carbon	1310:1315	arg1	preparation					1288:1298	preparation	1288:1298	preparation of porous carbon and supercapacitors	1288:1335	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	10	40	theme	carbon	1310:1315	arg1	development					1252:1262	the development	1248:1262	the development of biomass utilization	1248:1285	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	8	41	theme	specific	966:973	arg1	area					983:986	high specific surface area	961:986	high specific surface area with high-volume angstrom-sized pores in carbon matrix	961:1041	OPC shows high specific surface area with high-volume angstrom-sized pores in carbon matrix.					
29982026	10	42	theme	carbon	1206:1211	arg1	biomasses					1218:1226	porous carbon from biomasses	1199:1226	porous carbon from biomasses	1199:1226	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	7	43	theme	OPC	830:832	arg1	performances					814:825	The supercapacitive performances	794:825	The supercapacitive performances of OPC	794:832	The supercapacitive performances of OPC were investigated by cyclic voltammetry, electrochemical impedance spectroscopy, and galvanostatic charge-discharge.					
29982026	8	44	theme	high	961:964	arg1	area					983:986	high specific surface area	961:986	high specific surface area with high-volume angstrom-sized pores in carbon matrix	961:1041	OPC shows high specific surface area with high-volume angstrom-sized pores in carbon matrix.					
29982026	2	45	theme	porous	338:343	arg1	carbon					345:350	hierarchical porous carbon	325:350	hierarchical porous carbon with high-volume angstrom-sized pores	325:388	In this paper, a facile method is proposed for the preparation of hierarchical porous carbon with high-volume angstrom-sized pores.					
29982026	9	46	theme	supercapacitor	1057:1070	arg1	materials					1082:1090	supercapacitor electrode materials	1057:1090	supercapacitor electrode materials	1057:1090	When used as supercapacitor electrode materials, OPC shows high specific capacitance and good cycling stability.					
29982026	10	47	theme	porous	1199:1204	arg1	biomasses					1218:1226	porous carbon from biomasses	1199:1226	porous carbon from biomasses	1199:1226	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	6	48	theme	electron	744:751	arg1	microscope					753:762	scanning electron microscope	735:762	scanning electron microscope	735:762	The morphology and porous structure of OPC were characterized by scanning electron microscope and N2 adsorption/desorption.					
29982026	2	49	theme	hierarchical	325:336	arg1	carbon					345:350	hierarchical porous carbon	325:350	hierarchical porous carbon with high-volume angstrom-sized pores	325:388	In this paper, a facile method is proposed for the preparation of hierarchical porous carbon with high-volume angstrom-sized pores.					
29982026	9	50	theme	electrode	1072:1080	arg1	materials					1082:1090	supercapacitor electrode materials	1057:1090	supercapacitor electrode materials	1057:1090	When used as supercapacitor electrode materials, OPC shows high specific capacitance and good cycling stability.					
29982026	2	51	with	carbon	345:350	arg1	pores					384:388	high-volume angstrom-sized pores	357:388	high-volume angstrom-sized pores	357:388	In this paper, a facile method is proposed for the preparation of hierarchical porous carbon with high-volume angstrom-sized pores.					
29982026	6	52	theme	scanning	735:742	arg1	microscope					753:762	scanning electron microscope	735:762	scanning electron microscope	735:762	The morphology and porous structure of OPC were characterized by scanning electron microscope and N2 adsorption/desorption.					
29982026	1	53	theme	angstrom-sized	129:142	arg1	pores					144:148	angstrom-sized pores	129:148	angstrom-sized pores	129:148	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	8	54	theme	surface	975:981	arg1	area					983:986	high specific surface area	961:986	high specific surface area with high-volume angstrom-sized pores in carbon matrix	961:1041	OPC shows high specific surface area with high-volume angstrom-sized pores in carbon matrix.					
29982026	8	55	theme	high-volume	993:1003	arg1	pores					1020:1024	high-volume angstrom-sized pores	993:1024	high-volume angstrom-sized pores in carbon matrix	993:1041	OPC shows high specific surface area with high-volume angstrom-sized pores in carbon matrix.					
29982026	10	56	theme	utilization	1275:1285	arg1	preparation					1288:1298	preparation	1288:1298	preparation of porous carbon and supercapacitors	1288:1335	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	10	56	theme	utilization	1275:1285	arg1	development					1252:1262	the development	1248:1262	the development of biomass utilization	1248:1285	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	1	57	theme	high	232:235	arg1	capacitance					246:256	high specific capacitance	232:256	high specific capacitance	232:256	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	2	58	theme	angstrom-sized	369:382	arg1	pores					384:388	high-volume angstrom-sized pores	357:388	high-volume angstrom-sized pores	357:388	In this paper, a facile method is proposed for the preparation of hierarchical porous carbon with high-volume angstrom-sized pores.					
29982026	10	59	theme	biomass	1267:1273	arg1	utilization					1275:1285	biomass utilization	1267:1285	biomass utilization	1267:1285	This paper opens a general way to prepare porous carbon from biomasses, which will promote the development of biomass utilization, preparation of porous carbon and supercapacitors.					
29982026	1	60	theme	specific	237:244	arg1	capacitance					246:256	high specific capacitance	232:256	high specific capacitance	232:256	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	0	61	theme	facile	59:64	arg1	method					66:71	a facile method	57:71	a facile method for supercapacitor applications	57:103	Hierarchical porous carbon prepared from biomass through a facile method for supercapacitor applications.					
29982026	1	62	from	applications	181:192	arg1	important					153:161	important	153:161	important	153:161	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	1	62	from	applications	181:192	arg1	carbon					117:122	The porous carbon	106:122	The porous carbon with angstrom-sized pores	106:148	The porous carbon with angstrom-sized pores is important in supercapacitor applications, because of its high pack density and high specific capacitance.					
29982026	2	63	theme	high-volume	357:367	arg1	pores					384:388	high-volume angstrom-sized pores	357:388	high-volume angstrom-sized pores	357:388	In this paper, a facile method is proposed for the preparation of hierarchical porous carbon with high-volume angstrom-sized pores.					
29180367	12	0	theme	β-xylosidase	2250:2261	arg1	treatment					2263:2271	secondary β-xylosidase treatment	2240:2271	secondary β-xylosidase treatment	2240:2271	Furthermore, limit products other than aldotriouronic acid were observed for these Arg296 mutants upon secondary β-xylosidase treatment.					
29180367	6	1	theme	4-O-methyl-glucuronic	1008:1028	arg1	substitutions					1046:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions	992:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides	992:1097	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	4	2	theme	recognition	641:651	arg1	mechanism					618:626	the mechanism	614:626	the mechanism of substrate recognition	614:651	While the functional differences have been described, the mechanism of substrate recognition is still unknown.					
29180367	4	2	theme	recognition	641:651	arg1	unknown					662:668	unknown	662:668	unknown	662:668	While the functional differences have been described, the mechanism of substrate recognition is still unknown.					
29180367	5	3	theme	GH30	709:712	arg1	endoxylanase					714:725	a putative GH30 endoxylanase	698:725	a putative GH30 endoxylanase	698:725	Therefore, a gene encoding a putative GH30 endoxylanase was cloned from Streptomyces turgidiscabies C56, and the recombinant enzyme was purified and characterized.					
29180367	20	4	theme	mechanism	3810:3818	arg1	understanding					3789:3801	a better understanding	3780:3801	a better understanding of the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases	3780:3892	Our study results in a better understanding of the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases.					
29180367	9	5	theme	fungal	1835:1840	arg1	endoxylanases					1847:1859	bacterial but not fungal GH30 endoxylanases	1817:1859	bacterial but not fungal GH30 endoxylanases	1817:1859	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	6	6	theme	turgidiscabies	891:904	arg1	A					873:873	GH30 glucuronoxylan-specific xylanase A	835:873	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A)	835:915	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	10	7	theme	glucuronic	1936:1945	arg1	substitutions					1952:1964	glucuronic acid substitutions	1936:1964	glucuronic acid substitutions	1936:1964	To help gain an understanding of the mechanism of how StXyn30A recognizes glucuronic acid substitutions, Arg296 mutant enzymes were studied.					
29180367	9	8	theme	StXyn30A	1629:1636	arg1	modeling					1617:1624	molecular modeling	1607:1624	molecular modeling of StXyn30A	1607:1636	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	9	8	theme	StXyn30A	1629:1636	arg1	studies					1569:1575	previous structural studies	1549:1575	previous structural studies of bacterial GH30 enzymes	1549:1601	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	20	9	theme	substrate	3823:3831	arg1	specificity					3833:3843	specificity	3833:3843	specificity	3833:3843	Our study results in a better understanding of the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases.					
29180367	14	10	theme	industry.IMPORTANCE	2716:2734	arg1	Hemicellulases					2736:2749	industry.IMPORTANCE Hemicellulases	2716:2749	industry.IMPORTANCE Hemicellulases	2716:2749	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	9	11	theme	endoxylanases	1847:1859	arg1	endoxylanases					1847:1859	bacterial but not fungal GH30 endoxylanases	1817:1859	bacterial but not fungal GH30 endoxylanases	1817:1859	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	9	11	theme	endoxylanases	1847:1859	arg1	subsite					1803:1809	subsite -2	1803:1812	subsite -2 of bacterial but not fungal GH30 endoxylanases	1803:1859	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	16	12	theme	general	3150:3156	arg1	utility					3158:3164	their general utility	3144:3164	their general utility in xylan degradation	3144:3185	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	14	13	theme	GH30	2607:2610	arg1	endoxylanases					2612:2624	bacterial GH30 endoxylanases	2597:2624	bacterial GH30 endoxylanases	2597:2624	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	0	14	theme	GH30	0:3	arg1	Xylanase					29:36	GH30 Glucuronoxylan-Specific Xylanase	0:36	GH30 Glucuronoxylan-Specific Xylanase from Streptomyces	0:54	GH30 Glucuronoxylan-Specific Xylanase from Streptomyces turgidiscabies C56.					
29180367	15	15	theme	β-1,4-xylose	2948:2959	arg1	chain					2966:2970	the β-1,4-xylose main chain	2944:2970	the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass	2944:3045	These hemicellulases include endoxylanases that cleave the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass.					
29180367	12	16	theme	aldotriouronic	2176:2189	arg1	acid					2191:2194	aldotriouronic acid	2176:2194	limit products other than aldotriouronic acid	2150:2194	Furthermore, limit products other than aldotriouronic acid were observed for these Arg296 mutants upon secondary β-xylosidase treatment.					
29180367	6	17	theme	glucuronic	972:981	arg1	acid					983:986	glucuronic acid	972:986	glucuronic acid	972:986	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	14	18	theme	important	2755:2763	arg1	enzymes					2765:2771	important enzymes	2755:2771	important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism	2755:2886	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	7	19	theme	limit	1213:1217	arg1	structure					1227:1235	The StXyn30A limit product structure	1200:1235	The StXyn30A limit product structure	1200:1235	The StXyn30A limit product structure was analyzed following a secondary β-xylosidase treatment by thin-layer chromatography and mass spectrometry analysis.					
29180367	6	20	theme	xylanase	864:871	arg1	A					873:873	GH30 glucuronoxylan-specific xylanase A	835:873	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A)	835:915	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	2	21	theme	recognition	358:368	arg1	mechanism					335:343	the mechanism	331:343	the mechanism of substrate recognition by these enzymes	331:385	For effective biomass utilization, it is important to understand the mechanism of substrate recognition by these enzymes.					
29180367	19	22	with	comparison	3696:3705	arg1	endoxylanases					3744:3756	previously reported fungal GH30 endoxylanases	3712:3756	previously reported fungal GH30 endoxylanases	3712:3756	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	9	23	theme	conserved	1655:1663	arg1	residue					1674:1680	a conserved arginine residue	1653:1680	a conserved arginine residue (Arg296)	1653:1689	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	9	23	theme	conserved	1655:1663	arg1	Arg296					1683:1688	Arg296	1683:1688	Arg296	1683:1688	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	14	24	dep	allow	2495:2499	arg1	enzymes					2765:2771	important enzymes	2755:2771	important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism	2755:2886	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	18	25	theme	recognition	3570:3580	arg1	mechanisms					3546:3555	the mechanisms	3542:3555	the mechanisms of substrate recognition	3542:3580	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	13	26	theme	Arg296	2339:2344	arg1	interaction					2346:2356	the highly conserved Arg296 interaction	2318:2356	the highly conserved Arg296 interaction	2318:2356	These results indicate that a disruption of the highly conserved Arg296 interaction leads to a decrease of functional specificity in StXyn30A, as indicated by the detection of alternative hydrolysis products.					
29180367	1	27	theme	lignocellulosic	241:255	arg1	biomass					257:263	lignocellulosic biomass	241:263	lignocellulosic biomass	241:263	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	12	28	theme	limit	2150:2154	arg1	products					2156:2163	limit products	2150:2163	limit products other than aldotriouronic acid	2150:2194	Furthermore, limit products other than aldotriouronic acid were observed for these Arg296 mutants upon secondary β-xylosidase treatment.					
29180367	8	29	theme	main-chain	1457:1466	arg1	substitutions					1468:1480	these main-chain substitutions	1451:1480	these main-chain substitutions	1451:1480	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	11	30	theme	hydrolytic	2022:2031	arg1	activities					2033:2042	The glucuronoxylan hydrolytic activities	2003:2042	The glucuronoxylan hydrolytic activities of Arg296 mutants	2003:2060	The glucuronoxylan hydrolytic activities of Arg296 mutants were significantly reduced in comparison to those of the wild-type enzyme.					
29180367	2	31	theme	biomass	280:286	arg1	utilization					288:298	effective biomass utilization	270:298	effective biomass utilization	270:298	For effective biomass utilization, it is important to understand the mechanism of substrate recognition by these enzymes.					
29180367	18	32	theme	glycoside	3468:3476	arg1	family					3488:3493	glycoside hydrolase family 30	3468:3496	glycoside hydrolase family 30 (GH30)	3468:3503	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	18	32	theme	glycoside	3468:3476	arg1	GH30					3499:3502	GH30	3499:3502	GH30	3499:3502	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	13	33	theme	specificity	2392:2402	arg1	decrease					2369:2376	a decrease	2367:2376	a decrease of functional specificity in StXyn30A	2367:2414	These results indicate that a disruption of the highly conserved Arg296 interaction leads to a decrease of functional specificity in StXyn30A, as indicated by the detection of alternative hydrolysis products.					
29180367	6	34	theme	side-chain	1035:1044	arg1	substitutions					1046:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions	992:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides	992:1097	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	13	35	theme	products	2473:2480	arg1	detection					2437:2445	the detection	2433:2445	the detection of alternative hydrolysis products	2433:2480	These results indicate that a disruption of the highly conserved Arg296 interaction leads to a decrease of functional specificity in StXyn30A, as indicated by the detection of alternative hydrolysis products.					
29180367	16	36	theme	biomass	3113:3119	arg1	utilization					3092:3102	the utilization	3088:3102	the utilization of plant biomass	3088:3119	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	6	37	contain	containing	956:965	arg1	xylans					949:954	xylans	949:954	xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides	949:1097	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	6	37	contain	containing	956:965	arg2	substitutions					1046:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions	992:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides	992:1097	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	6	37	contain	containing	956:965	arg2	acid					983:986	glucuronic acid	972:986	glucuronic acid	972:986	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	18	38	from	difference	3528:3537	arg1	mechanisms					3546:3555	the mechanisms	3542:3555	the mechanisms of substrate recognition	3542:3580	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	5	39	theme	turgidiscabies	756:769	arg1	C56					771:773	Streptomyces turgidiscabies C56	743:773	Streptomyces turgidiscabies C56	743:773	Therefore, a gene encoding a putative GH30 endoxylanase was cloned from Streptomyces turgidiscabies C56, and the recombinant enzyme was purified and characterized.					
29180367	17	40	theme	substrate	3323:3331	arg1	recognition					3333:3343	substrate recognition	3323:3343	substrate recognition	3323:3343	For this, it is important to understand the mechanism of substrate recognition.					
29180367	20	41	theme	recognition	3849:3859	arg1	mechanism					3810:3818	the mechanism	3806:3818	the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases	3806:3892	Our study results in a better understanding of the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases.					
29180367	8	42	theme	second	1489:1494	arg1	residue					1510:1516	the second xylopyranosyl residue	1485:1516	the second xylopyranosyl residue from the reducing end	1485:1538	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	16	43	theme	defined	3220:3226	arg1	compositions					3228:3239	defined compositions	3220:3239	defined compositions of xylooligosaccharides	3220:3263	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	20	44	theme	bacterial	3865:3873	arg1	endoxylanases					3880:3892	bacterial GH30 endoxylanases	3865:3892	bacterial GH30 endoxylanases	3865:3892	Our study results in a better understanding of the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases.					
29180367	14	45	theme	glucuronoxylan	2544:2557	arg1	recognition					2559:2569	glucuronoxylan recognition	2544:2569	glucuronoxylan recognition	2544:2569	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	9	46	theme	acid	1721:1724	arg1	group					1746:1750	the glucuronic acid side-chain carboxyl group	1706:1750	the glucuronic acid side-chain carboxyl group	1706:1750	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	12	47	dep	other	2165:2169	arg1	than					2171:2174	than	2171:2174	than	2171:2174	Furthermore, limit products other than aldotriouronic acid were observed for these Arg296 mutants upon secondary β-xylosidase treatment.					
29180367	14	48	theme	mechanism	2531:2539	arg1	understanding					2510:2522	a better understanding	2501:2522	a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases	2501:2624	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	19	49	theme	endoxylanase	3679:3690	arg1	characterization					3613:3628	characterization	3613:3628	characterization	3613:3628	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	19	49	theme	endoxylanase	3679:3690	arg1	analyses					3646:3653	mutagenesis analyses	3634:3653	mutagenesis analyses	3634:3653	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	14	50	theme	specificity	2582:2592	arg1	mechanism					2531:2539	the mechanism	2527:2539	the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases	2527:2624	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	9	51	theme	carboxyl	1737:1744	arg1	group					1746:1750	the glucuronic acid side-chain carboxyl group	1706:1750	the glucuronic acid side-chain carboxyl group	1706:1750	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	19	52	theme	bacterial	3664:3672	arg1	endoxylanase					3679:3690	a new bacterial GH30 endoxylanase	3658:3690	a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases	3658:3756	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	15	53	theme	predominant	2986:2996	arg1	xylan					2975:2979	xylan	2975:2979	xylan	2975:2979	These hemicellulases include endoxylanases that cleave the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass.					
29180367	15	53	theme	predominant	2986:2996	arg1	form					2998:3001	the predominant form	2982:3001	the predominant form of hemicellulose	2982:3018	These hemicellulases include endoxylanases that cleave the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass.					
29180367	17	54	theme	recognition	3333:3343	arg1	mechanism					3310:3318	the mechanism	3306:3318	the mechanism of substrate recognition	3306:3343	For this, it is important to understand the mechanism of substrate recognition.					
29180367	20	55	theme	better	3782:3787	arg1	understanding					3789:3801	a better understanding	3780:3801	a better understanding of the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases	3780:3892	Our study results in a better understanding of the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases.					
29180367	14	56	theme	microbial	2850:2858	arg1	assimilation					2860:2871	eventual microbial assimilation	2841:2871	eventual microbial assimilation	2841:2871	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	14	57	theme	better	2503:2508	arg1	understanding					2510:2522	a better understanding	2501:2522	a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases	2501:2624	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	3	58	theme	glycoside	501:509	arg1	family					521:526	glycoside hydrolase family 30	501:529	glycoside hydrolase family 30 (GH30)	501:536	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	3	58	theme	glycoside	501:509	arg1	GH30					532:535	GH30	532:535	GH30	532:535	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	13	59	theme	functional	2381:2390	arg1	specificity					2392:2402	functional specificity	2381:2402	functional specificity	2381:2402	These results indicate that a disruption of the highly conserved Arg296 interaction leads to a decrease of functional specificity in StXyn30A, as indicated by the detection of alternative hydrolysis products.					
29180367	16	60	dep	utility	3158:3164	arg1	addition					3132:3139	addition	3132:3139	addition	3132:3139	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	0	61	from	Streptomyces	43:54	arg1	Xylanase					29:36	GH30 Glucuronoxylan-Specific Xylanase	0:36	GH30 Glucuronoxylan-Specific Xylanase from Streptomyces	0:54	GH30 Glucuronoxylan-Specific Xylanase from Streptomyces turgidiscabies C56.					
29180367	7	62	theme	mass	1328:1331	arg1	spectrometry					1333:1344	mass spectrometry	1328:1344	mass spectrometry	1328:1344	The StXyn30A limit product structure was analyzed following a secondary β-xylosidase treatment by thin-layer chromatography and mass spectrometry analysis.					
29180367	18	63	theme	Recent	3346:3351	arg1	studies					3353:3359	Recent studies	3346:3359	Recent studies	3346:3359	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	1	64	theme	bioenergy	115:123	arg1	research					125:132	bioenergy research	115:132	bioenergy research	115:132	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	13	65	theme	alternative	2450:2460	arg1	products					2473:2480	alternative hydrolysis products	2450:2480	alternative hydrolysis products	2450:2480	These results indicate that a disruption of the highly conserved Arg296 interaction leads to a decrease of functional specificity in StXyn30A, as indicated by the detection of alternative hydrolysis products.					
29180367	14	66	theme	eventual	2841:2848	arg1	assimilation					2860:2871	eventual microbial assimilation	2841:2871	eventual microbial assimilation	2841:2871	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	6	67	theme	side	1172:1175	arg1	chains					1177:1182	glucuronic acid side chains	1156:1182	glucuronic acid side chains	1156:1182	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	3	68	theme	fungal	464:469	arg1	endoxylanases					471:483	bacterial and fungal endoxylanases	450:483	bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30)	450:536	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	19	69	theme	reported	3723:3730	arg1	endoxylanases					3744:3756	previously reported fungal GH30 endoxylanases	3712:3756	previously reported fungal GH30 endoxylanases	3712:3756	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	21	70	theme	structure	4002:4010	arg1	definition					3984:3993	further definition	3976:3993	further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research	3976:4089	The experimental approach and resulting data support the conclusions and provide further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research.					
29180367	11	71	theme	wild-type	2119:2127	arg1	enzyme					2129:2134	the wild-type enzyme	2115:2134	the wild-type enzyme	2115:2134	The glucuronoxylan hydrolytic activities of Arg296 mutants were significantly reduced in comparison to those of the wild-type enzyme.					
29180367	9	72	theme	previous	1549:1556	arg1	studies					1569:1575	previous structural studies	1549:1575	previous structural studies of bacterial GH30 enzymes	1549:1601	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	6	73	theme	glucuronic	1156:1165	arg1	chains					1177:1182	glucuronic acid side chains	1156:1182	glucuronic acid side chains	1156:1182	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	3	74	theme	bacterial	450:458	arg1	endoxylanases					471:483	bacterial and fungal endoxylanases	450:483	bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30)	450:536	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	21	75	theme	endoxylanases	4033:4045	arg1	function					4016:4023	function	4016:4023	function	4016:4023	The experimental approach and resulting data support the conclusions and provide further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research.					
29180367	21	75	theme	endoxylanases	4033:4045	arg1	structure					4002:4010	structure	4002:4010	structure	4002:4010	The experimental approach and resulting data support the conclusions and provide further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research.					
29180367	11	76	theme	mutants	2054:2060	arg1	activities					2033:2042	The glucuronoxylan hydrolytic activities	2003:2042	The glucuronoxylan hydrolytic activities of Arg296 mutants	2003:2060	The glucuronoxylan hydrolytic activities of Arg296 mutants were significantly reduced in comparison to those of the wild-type enzyme.					
29180367	7	77	theme	β-xylosidase	1272:1283	arg1	treatment					1285:1293	a secondary β-xylosidase treatment	1260:1293	a secondary β-xylosidase treatment	1260:1293	The StXyn30A limit product structure was analyzed following a secondary β-xylosidase treatment by thin-layer chromatography and mass spectrometry analysis.					
29180367	9	78	theme	GH30	1842:1845	arg1	endoxylanases					1847:1859	bacterial but not fungal GH30 endoxylanases	1817:1859	bacterial but not fungal GH30 endoxylanases	1817:1859	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	14	79	theme	enzymes	2673:2679	arg1	definition					2646:2655	further definition	2638:2655	further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases	2638:2749	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	9	80	theme	bacterial	1580:1588	arg1	enzymes					1595:1601	bacterial GH30 enzymes	1580:1601	bacterial GH30 enzymes	1580:1601	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	14	81	theme	hemicellulosic	2788:2801	arg1	polysaccharides					2803:2817	hemicellulosic polysaccharides	2788:2817	hemicellulosic polysaccharides	2788:2817	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	14	82	theme	potential	2691:2699	arg1	application					2701:2711	their potential application	2685:2711	their potential application in industry.IMPORTANCE Hemicellulases	2685:2749	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	18	83	theme	fungal	3422:3427	arg1	endoxylanases					3429:3441	bacterial and fungal endoxylanases	3408:3441	bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30)	3408:3503	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	16	84	theme	important	3070:3078	arg1	role					3080:3083	an important role	3067:3083	an important role	3067:3083	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	15	85	from	chain	2966:2970	arg1	biomass					3039:3045	lignocellulosic biomass	3023:3045	lignocellulosic biomass	3023:3045	These hemicellulases include endoxylanases that cleave the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass.					
29180367	9	86	theme	enzymes	1595:1601	arg1	modeling					1617:1624	molecular modeling	1607:1624	molecular modeling of StXyn30A	1607:1636	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	9	86	theme	enzymes	1595:1601	arg1	studies					1569:1575	previous structural studies	1549:1575	previous structural studies of bacterial GH30 enzymes	1549:1601	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	15	87	theme	lignocellulosic	3023:3037	arg1	biomass					3039:3045	lignocellulosic biomass	3023:3045	lignocellulosic biomass	3023:3045	These hemicellulases include endoxylanases that cleave the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass.					
29180367	6	88	theme	acid	1030:1033	arg1	substitutions					1046:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions	992:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides	992:1097	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	9	89	theme	bacterial	1817:1825	arg1	endoxylanases					1847:1859	bacterial but not fungal GH30 endoxylanases	1817:1859	bacterial but not fungal GH30 endoxylanases	1817:1859	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	5	90	theme	putative	700:707	arg1	endoxylanase					714:725	a putative GH30 endoxylanase	698:725	a putative GH30 endoxylanase	698:725	Therefore, a gene encoding a putative GH30 endoxylanase was cloned from Streptomyces turgidiscabies C56, and the recombinant enzyme was purified and characterized.					
29180367	10	91	theme	mechanism	1899:1907	arg1	understanding					1878:1890	an understanding	1875:1890	an understanding of the mechanism of how StXyn30A recognizes glucuronic acid substitutions	1875:1964	To help gain an understanding of the mechanism of how StXyn30A recognizes glucuronic acid substitutions, Arg296 mutant enzymes were studied.					
29180367	1	92	from	enzymes	104:110	arg1	research					125:132	bioenergy research	115:132	bioenergy research	115:132	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	9	93	theme	molecular	1607:1615	arg1	modeling					1617:1624	molecular modeling	1607:1624	molecular modeling of StXyn30A	1607:1636	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	12	94	theme	secondary	2240:2248	arg1	treatment					2263:2271	secondary β-xylosidase treatment	2240:2271	secondary β-xylosidase treatment	2240:2271	Furthermore, limit products other than aldotriouronic acid were observed for these Arg296 mutants upon secondary β-xylosidase treatment.					
29180367	6	95	theme	common	1001:1006	arg1	substitutions					1046:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions	992:1058	the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides	992:1097	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	4	96	theme	substrate	631:639	arg1	recognition					641:651	substrate recognition	631:651	substrate recognition	631:651	While the functional differences have been described, the mechanism of substrate recognition is still unknown.					
29180367	16	97	theme	xylooligosaccharides	3244:3263	arg1	compositions					3228:3239	defined compositions	3220:3239	defined compositions of xylooligosaccharides	3220:3263	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	21	98	theme	experimental	3899:3910	arg1	approach					3912:3919	The experimental approach	3895:3919	The experimental approach	3895:3919	The experimental approach and resulting data support the conclusions and provide further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research.					
29180367	10	99	theme	acid	1947:1950	arg1	substitutions					1952:1964	glucuronic acid substitutions	1936:1964	glucuronic acid substitutions	1936:1964	To help gain an understanding of the mechanism of how StXyn30A recognizes glucuronic acid substitutions, Arg296 mutant enzymes were studied.					
29180367	16	100	from	utility	3158:3164	arg1	degradation					3175:3185	xylan degradation	3169:3185	xylan degradation	3169:3185	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	8	101	contain	have	1446:1449	arg1	products					1371:1378	The hydrolysis products	1356:1378	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A	1356:1444	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	8	101	contain	have	1446:1449	arg2	substitutions					1468:1480	these main-chain substitutions	1451:1480	these main-chain substitutions	1451:1480	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	9	102	theme	side-chain	1726:1735	arg1	group					1746:1750	the glucuronic acid side-chain carboxyl group	1706:1750	the glucuronic acid side-chain carboxyl group	1706:1750	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	7	103	theme	product	1219:1225	arg1	structure					1227:1235	The StXyn30A limit product structure	1200:1235	The StXyn30A limit product structure	1200:1235	The StXyn30A limit product structure was analyzed following a secondary β-xylosidase treatment by thin-layer chromatography and mass spectrometry analysis.					
29180367	6	104	theme	Streptomyces	878:889	arg1	turgidiscabies					891:904	Streptomyces turgidiscabies	878:904	Streptomyces turgidiscabies (StXyn30A)	878:915	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	6	104	theme	Streptomyces	878:889	arg1	StXyn30A					907:914	StXyn30A	907:914	StXyn30A	907:914	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	7	105	theme	StXyn30A	1204:1211	arg1	structure					1227:1235	The StXyn30A limit product structure	1200:1235	The StXyn30A limit product structure	1200:1235	The StXyn30A limit product structure was analyzed following a secondary β-xylosidase treatment by thin-layer chromatography and mass spectrometry analysis.					
29180367	14	106	theme	bacterial	2597:2605	arg1	endoxylanases					2612:2624	bacterial GH30 endoxylanases	2597:2624	bacterial GH30 endoxylanases	2597:2624	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	10	107	theme	mutant	1974:1979	arg1	enzymes					1981:1987	Arg296 mutant enzymes	1967:1987	Arg296 mutant enzymes	1967:1987	To help gain an understanding of the mechanism of how StXyn30A recognizes glucuronic acid substitutions, Arg296 mutant enzymes were studied.					
29180367	15	108	theme	main	2961:2964	arg1	chain					2966:2970	the β-1,4-xylose main chain	2944:2970	the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass	2944:3045	These hemicellulases include endoxylanases that cleave the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass.					
29180367	1	109	theme	hemicellulose	215:227	arg1	fraction					229:236	the hemicellulose fraction	211:236	the hemicellulose fraction of lignocellulosic biomass	211:263	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	18	110	theme	bacterial	3408:3416	arg1	endoxylanases					3429:3441	bacterial and fungal endoxylanases	3408:3441	bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30)	3408:3503	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	21	111	from	application	4057:4067	arg1	research					4082:4089	bioenergy research	4072:4089	bioenergy research	4072:4089	The experimental approach and resulting data support the conclusions and provide further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research.					
29180367	6	112	theme	glucuronoxylan-specific	840:862	arg1	A					873:873	GH30 glucuronoxylan-specific xylanase A	835:873	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A)	835:915	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	2	113	theme	substrate	348:356	arg1	recognition					358:368	substrate recognition	348:368	substrate recognition by these enzymes	348:385	For effective biomass utilization, it is important to understand the mechanism of substrate recognition by these enzymes.					
29180367	9	114	theme	arginine	1665:1672	arg1	residue					1674:1680	a conserved arginine residue	1653:1680	a conserved arginine residue (Arg296)	1653:1689	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	9	114	theme	arginine	1665:1672	arg1	Arg296					1683:1688	Arg296	1683:1688	Arg296	1683:1688	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	6	115	theme	linear	1071:1076	arg1	xylooligosaccharides					1078:1097	linear xylooligosaccharides	1071:1097	linear xylooligosaccharides	1071:1097	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	21	116	theme	function	4016:4023	arg1	definition					3984:3993	further definition	3976:3993	further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research	3976:4089	The experimental approach and resulting data support the conclusions and provide further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research.					
29180367	16	117	theme	xylan	3169:3173	arg1	degradation					3175:3185	xylan degradation	3169:3185	xylan degradation	3169:3185	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	13	118	theme	interaction	2346:2356	arg1	disruption					2304:2313	a disruption	2302:2313	a disruption of the highly conserved Arg296 interaction	2302:2356	These results indicate that a disruption of the highly conserved Arg296 interaction leads to a decrease of functional specificity in StXyn30A, as indicated by the detection of alternative hydrolysis products.					
29180367	1	119	theme	biomass	257:263	arg1	fraction					229:236	the hemicellulose fraction	211:236	the hemicellulose fraction of lignocellulosic biomass	211:263	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	8	120	theme	hydrolysis	1360:1369	arg1	products					1371:1378	The hydrolysis products	1356:1378	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A	1356:1444	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	19	121	theme	mutagenesis	3634:3644	arg1	analyses					3646:3653	mutagenesis analyses	3634:3653	mutagenesis analyses	3634:3653	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	2	122	theme	effective	270:278	arg1	utilization					288:298	effective biomass utilization	270:298	effective biomass utilization	270:298	For effective biomass utilization, it is important to understand the mechanism of substrate recognition by these enzymes.					
29180367	8	123	from	4-O-methylglucuronoxylan	1409:1432	arg1	products					1371:1378	The hydrolysis products	1356:1378	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A	1356:1444	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	14	124	theme	enzyme	2575:2580	arg1	specificity					2582:2592	enzyme specificity	2575:2592	enzyme specificity	2575:2592	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	11	125	theme	glucuronoxylan	2007:2020	arg1	activities					2033:2042	The glucuronoxylan hydrolytic activities	2003:2042	The glucuronoxylan hydrolytic activities of Arg296 mutants	2003:2060	The glucuronoxylan hydrolytic activities of Arg296 mutants were significantly reduced in comparison to those of the wild-type enzyme.					
29180367	5	126	theme	Streptomyces	743:754	arg1	C56					771:773	Streptomyces turgidiscabies C56	743:773	Streptomyces turgidiscabies C56	743:773	Therefore, a gene encoding a putative GH30 endoxylanase was cloned from Streptomyces turgidiscabies C56, and the recombinant enzyme was purified and characterized.					
29180367	18	127	theme	endoxylanases	3429:3441	arg1	specificities					3391:3403	the substrate specificities	3377:3403	the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30)	3377:3503	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	18	127	theme	endoxylanases	3429:3441	arg1	distinct					3510:3517	distinct	3510:3517	distinct	3510:3517	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	8	128	from	glucuronoxylan	1390:1403	arg1	products					1371:1378	The hydrolysis products	1356:1378	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A	1356:1444	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	18	129	theme	substrate	3381:3389	arg1	specificities					3391:3403	the substrate specificities	3377:3403	the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30)	3377:3503	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	18	129	theme	substrate	3381:3389	arg1	distinct					3510:3517	distinct	3510:3517	distinct	3510:3517	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	16	130	theme	plant	3107:3111	arg1	biomass					3113:3119	plant biomass	3107:3119	plant biomass	3107:3119	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	14	131	theme	recognition	2559:2569	arg1	mechanism					2531:2539	the mechanism	2527:2539	the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases	2527:2624	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	19	132	theme	new	3660:3662	arg1	endoxylanase					3679:3690	a new bacterial GH30 endoxylanase	3658:3690	a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases	3658:3756	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	15	133	theme	hemicellulose	3006:3018	arg1	xylan					2975:2979	xylan	2975:2979	xylan	2975:2979	These hemicellulases include endoxylanases that cleave the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass.					
29180367	15	133	theme	hemicellulose	3006:3018	arg1	form					2998:3001	the predominant form	2982:3001	the predominant form of hemicellulose	2982:3018	These hemicellulases include endoxylanases that cleave the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass.					
29180367	13	134	theme	conserved	2329:2337	arg1	interaction					2346:2356	the highly conserved Arg296 interaction	2318:2356	the highly conserved Arg296 interaction	2318:2356	These results indicate that a disruption of the highly conserved Arg296 interaction leads to a decrease of functional specificity in StXyn30A, as indicated by the detection of alternative hydrolysis products.					
29180367	9	135	theme	glucuronic	1710:1719	arg1	group					1746:1750	the glucuronic acid side-chain carboxyl group	1706:1750	the glucuronic acid side-chain carboxyl group	1706:1750	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	3	136	theme	Recent	388:393	arg1	studies					395:401	Recent studies	388:401	Recent studies	388:401	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	8	137	theme	xylopyranosyl	1496:1508	arg1	residue					1510:1516	the second xylopyranosyl residue	1485:1516	the second xylopyranosyl residue from the reducing end	1485:1538	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	21	138	theme	resulting	3925:3933	arg1	data					3935:3938	resulting data	3925:3938	resulting data	3925:3938	The experimental approach and resulting data support the conclusions and provide further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research.					
29180367	1	139	theme	predominant	181:191	arg1	xylan					170:174	xylan	170:174	xylan	170:174	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	1	139	theme	predominant	181:191	arg1	polysaccharide					193:206	the predominant polysaccharide	177:206	the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass	177:263	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	16	140	used	used	3205:3208	arg2	they					3188:3191	they	3188:3191	they	3188:3191	Endoxylanases play an important role in the utilization of plant biomass because in addition to their general utility in xylan degradation, they can also be used to create defined compositions of xylooligosaccharides.					
29180367	4	141	theme	functional	570:579	arg1	differences					581:591	the functional differences	566:591	the functional differences	566:591	While the functional differences have been described, the mechanism of substrate recognition is still unknown.					
29180367	7	142	dep	chromatography	1309:1322	arg1	analysis					1346:1353	analysis	1346:1353	analysis	1346:1353	The StXyn30A limit product structure was analyzed following a secondary β-xylosidase treatment by thin-layer chromatography and mass spectrometry analysis.					
29180367	5	143	theme	recombinant	784:794	arg1	enzyme					796:801	the recombinant enzyme	780:801	the recombinant enzyme	780:801	Therefore, a gene encoding a putative GH30 endoxylanase was cloned from Streptomyces turgidiscabies C56, and the recombinant enzyme was purified and characterized.					
29180367	1	144	from	polysaccharide	193:206	arg1	fraction					229:236	the hemicellulose fraction	211:236	the hemicellulose fraction of lignocellulosic biomass	211:263	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	8	145	theme	reducing	1527:1534	arg1	end					1536:1538	the reducing end	1523:1538	the reducing end	1523:1538	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	19	146	theme	GH30	3674:3677	arg1	endoxylanase					3679:3690	a new bacterial GH30 endoxylanase	3658:3690	a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases	3658:3756	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	15	147	theme	xylan	2975:2979	arg1	chain					2966:2970	the β-1,4-xylose main chain	2944:2970	the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass	2944:3045	These hemicellulases include endoxylanases that cleave the β-1,4-xylose main chain of xylan, the predominant form of hemicellulose in lignocellulosic biomass.					
29180367	1	148	theme	important	94:102	arg1	Endoxylanases					76:88	Endoxylanases	76:88	Endoxylanases	76:88	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	1	148	theme	important	94:102	arg1	enzymes					104:110	important enzymes	94:110	important enzymes in bioenergy research	94:132	Endoxylanases are important enzymes in bioenergy research because they specifically hydrolyze xylan, the predominant polysaccharide in the hemicellulose fraction of lignocellulosic biomass.					
29180367	14	149	from	application	2701:2711	arg1	Hemicellulases					2736:2749	industry.IMPORTANCE Hemicellulases	2716:2749	industry.IMPORTANCE Hemicellulases	2716:2749	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	20	150	theme	specificity	3833:3843	arg1	mechanism					3810:3818	the mechanism	3806:3818	the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases	3806:3892	Our study results in a better understanding of the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases.					
29180367	3	151	theme	hydrolase	511:519	arg1	family					521:526	glycoside hydrolase family 30	501:529	glycoside hydrolase family 30 (GH30)	501:536	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	3	151	theme	hydrolase	511:519	arg1	GH30					532:535	GH30	532:535	GH30	532:535	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	0	152	theme	Glucuronoxylan-Specific	5:27	arg1	Xylanase					29:36	GH30 Glucuronoxylan-Specific Xylanase	0:36	GH30 Glucuronoxylan-Specific Xylanase from Streptomyces	0:54	GH30 Glucuronoxylan-Specific Xylanase from Streptomyces turgidiscabies C56.					
29180367	19	153	theme	GH30	3739:3742	arg1	endoxylanases					3744:3756	previously reported fungal GH30 endoxylanases	3712:3756	previously reported fungal GH30 endoxylanases	3712:3756	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	7	154	theme	thin-layer	1298:1307	arg1	chromatography					1309:1322	thin-layer chromatography	1298:1322	thin-layer chromatography	1298:1322	The StXyn30A limit product structure was analyzed following a secondary β-xylosidase treatment by thin-layer chromatography and mass spectrometry analysis.					
29180367	21	155	theme	bioenergy	4072:4080	arg1	research					4082:4089	bioenergy research	4072:4089	bioenergy research	4072:4089	The experimental approach and resulting data support the conclusions and provide further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research.					
29180367	18	156	theme	substrate	3560:3568	arg1	recognition					3570:3580	substrate recognition	3560:3580	substrate recognition	3560:3580	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	6	157	theme	chains	1177:1182	arg1	recognition					1141:1151	the recognition	1137:1151	the recognition of glucuronic acid side chains for hydrolysis	1137:1197	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	3	158	theme	substrate	423:431	arg1	specificities					433:445	the substrate specificities	419:445	the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30)	419:536	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	3	158	theme	substrate	423:431	arg1	different					549:557	different	549:557	different	549:557	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	13	159	theme	hydrolysis	2462:2471	arg1	products					2473:2480	alternative hydrolysis products	2450:2480	alternative hydrolysis products	2450:2480	These results indicate that a disruption of the highly conserved Arg296 interaction leads to a decrease of functional specificity in StXyn30A, as indicated by the detection of alternative hydrolysis products.					
29180367	18	160	theme	hydrolase	3478:3486	arg1	family					3488:3493	glycoside hydrolase family 30	3468:3496	glycoside hydrolase family 30 (GH30)	3468:3503	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	18	160	theme	hydrolase	3478:3486	arg1	GH30					3499:3502	GH30	3499:3502	GH30	3499:3502	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases that are classified into glycoside hydrolase family 30 (GH30) were distinct, but the difference in the mechanisms of substrate recognition is still unknown.					
29180367	6	161	theme	acid	1167:1170	arg1	chains					1177:1182	glucuronic acid side chains	1156:1182	glucuronic acid side chains	1156:1182	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	3	162	theme	endoxylanases	471:483	arg1	specificities					433:445	the substrate specificities	419:445	the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30)	419:536	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	3	162	theme	endoxylanases	471:483	arg1	different					549:557	different	549:557	different	549:557	Recent studies have shown that the substrate specificities of bacterial and fungal endoxylanases classified into glycoside hydrolase family 30 (GH30) were quite different.					
29180367	13	163	from	decrease	2369:2376	arg1	StXyn30A					2407:2414	StXyn30A	2407:2414	StXyn30A	2407:2414	These results indicate that a disruption of the highly conserved Arg296 interaction leads to a decrease of functional specificity in StXyn30A, as indicated by the detection of alternative hydrolysis products.					
29180367	6	164	theme	GH30	835:838	arg1	A					873:873	GH30 glucuronoxylan-specific xylanase A	835:873	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A)	835:915	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	7	165	theme	secondary	1262:1270	arg1	treatment					1285:1293	a secondary β-xylosidase treatment	1260:1293	a secondary β-xylosidase treatment	1260:1293	The StXyn30A limit product structure was analyzed following a secondary β-xylosidase treatment by thin-layer chromatography and mass spectrometry analysis.					
29180367	9	166	theme	structural	1558:1567	arg1	studies					1569:1575	previous structural studies	1549:1575	previous structural studies of bacterial GH30 enzymes	1549:1601	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	14	167	theme	smaller	2822:2828	arg1	sugars					2830:2835	smaller sugars	2822:2835	smaller sugars for eventual microbial assimilation and metabolism	2822:2886	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	20	168	theme	GH30	3875:3878	arg1	endoxylanases					3880:3892	bacterial GH30 endoxylanases	3865:3892	bacterial GH30 endoxylanases	3865:3892	Our study results in a better understanding of the mechanism of substrate specificity and recognition for bacterial GH30 endoxylanases.					
29180367	6	169	theme	hydrolytic	924:933	arg1	activity					935:942	hydrolytic activity	924:942	hydrolytic activity	924:942	GH30 glucuronoxylan-specific xylanase A of Streptomyces turgidiscabies (StXyn30A) showed hydrolytic activity with xylans containing both glucuronic acid and the more common 4-O-methyl-glucuronic acid side-chain substitutions but not on linear xylooligosaccharides, suggesting that this enzyme requires the recognition of glucuronic acid side chains for hydrolysis.					
29180367	19	170	theme	fungal	3732:3737	arg1	endoxylanases					3744:3756	previously reported fungal GH30 endoxylanases	3712:3756	previously reported fungal GH30 endoxylanases	3712:3756	We performed characterization and mutagenesis analyses of a new bacterial GH30 endoxylanase for comparison with previously reported fungal GH30 endoxylanases.					
29180367	14	171	theme	unique	2666:2671	arg1	enzymes					2673:2679	these unique enzymes	2660:2679	these unique enzymes	2660:2679	Our studies allow a better understanding of the mechanism of glucuronoxylan recognition and enzyme specificity by bacterial GH30 endoxylanases and provide further definition of these unique enzymes for their potential application in industry.IMPORTANCE Hemicellulases are important enzymes that hydrolyze hemicellulosic polysaccharides to smaller sugars for eventual microbial assimilation and metabolism.					
29180367	11	172	theme	Arg296	2047:2052	arg1	mutants					2054:2060	Arg296 mutants	2047:2060	Arg296 mutants	2047:2060	The glucuronoxylan hydrolytic activities of Arg296 mutants were significantly reduced in comparison to those of the wild-type enzyme.					
29180367	8	173	from	end	1536:1538	arg1	residue					1510:1516	the second xylopyranosyl residue	1485:1516	the second xylopyranosyl residue from the reducing end	1485:1538	The hydrolysis products from both glucuronoxylan and 4-O-methylglucuronoxylan by StXyn30A have these main-chain substitutions on the second xylopyranosyl residue from the reducing end.					
29180367	9	174	theme	GH30	1590:1593	arg1	enzymes					1595:1601	bacterial GH30 enzymes	1580:1601	bacterial GH30 enzymes	1580:1601	Because previous structural studies of bacterial GH30 enzymes and molecular modeling of StXyn30A suggested that a conserved arginine residue (Arg296) interacts with the glucuronic acid side-chain carboxyl group, we focused on this residue, which is conserved at subsite -2 of bacterial but not fungal GH30 endoxylanases.					
29180367	21	175	dep	structure	4002:4010	arg1	the					3998:4000	the	3998:4000	the	3998:4000	The experimental approach and resulting data support the conclusions and provide further definition of the structure and function of GH30 endoxylanases for their application in bioenergy research.					
31260951	0	0	theme	unripe	94:99	arg1	flour					110:114	whole unripe plantain flour	88:114	whole unripe plantain flour	88:114	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.					
31260951	7	1	theme	gluten-free	914:924	arg1	spaghetti					926:934	gluten-free spaghetti	914:934	gluten-free spaghetti having highest content of fiber and lower starch digestion rates	914:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	5	2	theme	swelling	686:693	arg1	reduction					654:662	the reduction	650:662	the reduction of the starch granule swelling in the cooking process	650:716	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	0	3	theme	whole	88:92	arg1	flour					110:114	whole unripe plantain flour	88:114	whole unripe plantain flour	88:114	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.					
31260951	4	4	theme	spaghetti	525:533	arg1	formulations					535:546	spaghetti formulations	525:546	spaghetti formulations	525:546	Chemical composition, cooking quality, texture analysis, and microstructure of spaghetti formulations were analyzed.					
31260951	3	5	dep	ingredient	391:400	arg1	pulp					413:416	pulp	413:416	pulp	413:416	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	3	5	dep	ingredient	391:400	arg1	whole					403:407	whole	403:407	whole	403:407	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	5	6	theme	fiber	618:622	arg1	presence					606:613	the presence	602:613	the presence of fiber in the food matrix	602:641	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	7	7	theme	good	895:898	arg1	potential					900:908	good potential	895:908	good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates	895:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	0	8	theme	flour	110:114	arg1	spaghetti					75:83	gluten-free spaghetti	63:83	gluten-free spaghetti of whole unripe plantain flour	63:114	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.					
31260951	5	9	theme	starch	671:676	arg1	swelling					686:693	the starch granule swelling	667:693	the starch granule swelling	667:693	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	7	10	theme	starch	978:983	arg1	rates					995:999	lower starch digestion rates	972:999	lower starch digestion rates	972:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	2	11	theme	starch	308:313	arg1	fraction					315:322	undigestible starch fraction	295:322	undigestible starch fraction	295:322	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	0	12	theme	plantain	101:108	arg1	flour					110:114	whole unripe plantain flour	88:114	whole unripe plantain flour	88:114	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.					
31260951	4	13	theme	texture	485:491	arg1	analysis					493:500	texture analysis	485:500	texture analysis	485:500	Chemical composition, cooking quality, texture analysis, and microstructure of spaghetti formulations were analyzed.					
31260951	5	14	theme	granule	678:684	arg1	swelling					686:693	the starch granule swelling	667:693	the starch granule swelling	667:693	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	6	15	theme	starch	826:831	arg1	fractions					833:841	increased resistant starch fractions	806:841	increased resistant starch fractions	806:841	Spaghetti made with whole plantain flour exhibited lower rapidly starch fraction, with increased resistant starch fractions.					
31260951	2	16	theme	undigestible	295:306	arg1	fraction					315:322	undigestible starch fraction	295:322	undigestible starch fraction	295:322	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	3	17	from	ingredient	391:400	arg1	formulations					432:443	spaghetti formulations	422:443	spaghetti formulations	422:443	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	7	18	contain	having	936:941	arg2	content					951:957	highest content	943:957	highest content of fiber and lower starch digestion rates	943:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	7	18	contain	having	936:941	arg1	spaghetti					926:934	gluten-free spaghetti	914:934	gluten-free spaghetti having highest content of fiber and lower starch digestion rates	914:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	5	19	theme	food	631:634	arg1	matrix					636:641	the food matrix	627:641	the food matrix	627:641	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	1	20	theme	several	178:184	arg1	regions					195:201	several tropical regions	178:201	several tropical regions	178:201	Plantain is a climacteric fruit having economic relevance in several tropical regions.					
31260951	6	21	theme	increased	806:814	arg1	fractions					833:841	increased resistant starch fractions	806:841	increased resistant starch fractions	806:841	Spaghetti made with whole plantain flour exhibited lower rapidly starch fraction, with increased resistant starch fractions.					
31260951	7	22	theme	unripe	863:868	arg1	flour					879:883	the whole unripe plantain flour	853:883	the whole unripe plantain flour	853:883	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	2	23	dep	carbohydrates	261:273	arg1	fibre					284:288	fibre	284:288	fibre	284:288	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	1	24	contain	having	149:154	arg2	relevance					165:173	economic relevance	156:173	economic relevance	156:173	Plantain is a climacteric fruit having economic relevance in several tropical regions.					
31260951	1	24	contain	having	149:154	arg1	fruit					143:147	a climacteric fruit	129:147	a climacteric fruit having economic relevance in several tropical regions	129:201	Plantain is a climacteric fruit having economic relevance in several tropical regions.					
31260951	1	24	contain	having	149:154	arg1	Plantain					117:124	Plantain	117:124	Plantain	117:124	Plantain is a climacteric fruit having economic relevance in several tropical regions.					
31260951	7	25	theme	lower	972:976	arg1	rates					995:999	lower starch digestion rates	972:999	lower starch digestion rates	972:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	7	26	theme	plantain	870:877	arg1	flour					879:883	the whole unripe plantain flour	853:883	the whole unripe plantain flour	853:883	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	3	27	theme	spaghetti	422:430	arg1	formulations					432:443	spaghetti formulations	422:443	spaghetti formulations	422:443	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	0	28	theme	Physicochemical	0:14	arg1	analysis					51:58	Physicochemical, microstructural and digestibility analysis	0:58	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.	0:115	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.					
31260951	3	29	theme	alternative	379:389	arg1	flour					341:345	Unripe plantain flour	325:345	Unripe plantain flour	325:345	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	3	29	theme	alternative	379:389	arg1	ingredient					391:400	an alternative ingredient	376:400	an alternative ingredient (whole and pulp) in spaghetti formulations	376:443	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	5	30	from	reduction	654:662	arg1	process					710:716	the cooking process	698:716	the cooking process	698:716	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	6	31	theme	resistant	816:824	arg1	fractions					833:841	increased resistant starch fractions	806:841	increased resistant starch fractions	806:841	Spaghetti made with whole plantain flour exhibited lower rapidly starch fraction, with increased resistant starch fractions.					
31260951	7	32	theme	rates	995:999	arg1	content					951:957	highest content	943:957	highest content of fiber and lower starch digestion rates	943:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	0	33	theme	microstructural	17:31	arg1	analysis					51:58	Physicochemical, microstructural and digestibility analysis	0:58	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.	0:115	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.					
31260951	2	34	theme	fraction	315:322	arg1	plantain					211:218	Unripe plantain	204:218	Unripe plantain	204:218	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	2	34	theme	fraction	315:322	arg1	source					238:243	an alternative source	223:243	an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction	223:322	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	7	35	theme	digestion	985:993	arg1	rates					995:999	lower starch digestion rates	972:999	lower starch digestion rates	972:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	2	36	theme	indigestible	248:259	arg1	carbohydrates					261:273	indigestible carbohydrates	248:273	indigestible carbohydrates (dietary fibre)	248:289	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	0	37	theme	digestibility	37:49	arg1	analysis					51:58	Physicochemical, microstructural and digestibility analysis	0:58	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.	0:115	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.					
31260951	6	38	theme	starch	784:789	arg1	fraction					791:798	lower rapidly starch fraction	770:798	lower rapidly starch fraction	770:798	Spaghetti made with whole plantain flour exhibited lower rapidly starch fraction, with increased resistant starch fractions.					
31260951	0	39	theme	gluten-free	63:73	arg1	spaghetti					75:83	gluten-free spaghetti	63:83	gluten-free spaghetti of whole unripe plantain flour	63:114	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.					
31260951	4	40	theme	formulations	535:546	arg1	analysis					493:500	texture analysis	485:500	texture analysis	485:500	Chemical composition, cooking quality, texture analysis, and microstructure of spaghetti formulations were analyzed.					
31260951	4	40	theme	formulations	535:546	arg1	microstructure					507:520	microstructure	507:520	microstructure	507:520	Chemical composition, cooking quality, texture analysis, and microstructure of spaghetti formulations were analyzed.					
31260951	4	40	theme	formulations	535:546	arg1	composition					455:465	Chemical composition	446:465	Chemical composition	446:465	Chemical composition, cooking quality, texture analysis, and microstructure of spaghetti formulations were analyzed.					
31260951	4	40	theme	formulations	535:546	arg1	quality					476:482	cooking quality	468:482	cooking quality	468:482	Chemical composition, cooking quality, texture analysis, and microstructure of spaghetti formulations were analyzed.					
31260951	3	41	theme	Unripe	325:330	arg1	flour					341:345	Unripe plantain flour	325:345	Unripe plantain flour	325:345	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	3	41	theme	Unripe	325:330	arg1	ingredient					391:400	an alternative ingredient	376:400	an alternative ingredient (whole and pulp) in spaghetti formulations	376:443	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	4	42	theme	cooking	468:474	arg1	quality					476:482	cooking quality	468:482	cooking quality	468:482	Chemical composition, cooking quality, texture analysis, and microstructure of spaghetti formulations were analyzed.					
31260951	5	43	theme	microstructure	567:580	arg1	results					582:588	The microstructure results	563:588	The microstructure results	563:588	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	5	44	from	presence	606:613	arg1	matrix					636:641	the food matrix	627:641	the food matrix	627:641	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	5	45	attach	presence	606:613	arg1	matrix					636:641	the food matrix	627:641	the food matrix	627:641	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	5	45	attach	presence	606:613	arg2	fiber					618:622	fiber	618:622	fiber	618:622	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	3	46	theme	plantain	332:339	arg1	flour					341:345	Unripe plantain flour	325:345	Unripe plantain flour	325:345	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	3	46	theme	plantain	332:339	arg1	ingredient					391:400	an alternative ingredient	376:400	an alternative ingredient (whole and pulp) in spaghetti formulations	376:443	Unripe plantain flour was explored in this work as an alternative ingredient (whole and pulp) in spaghetti formulations.					
31260951	2	47	theme	alternative	226:236	arg1	plantain					211:218	Unripe plantain	204:218	Unripe plantain	204:218	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	2	47	theme	alternative	226:236	arg1	source					238:243	an alternative source	223:243	an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction	223:322	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	7	48	theme	highest	943:949	arg1	content					951:957	highest content	943:957	highest content of fiber and lower starch digestion rates	943:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	7	49	theme	fiber	962:966	arg1	content					951:957	highest content	943:957	highest content of fiber and lower starch digestion rates	943:999	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	1	50	theme	climacteric	131:141	arg1	fruit					143:147	a climacteric fruit	129:147	a climacteric fruit having economic relevance in several tropical regions	129:201	Plantain is a climacteric fruit having economic relevance in several tropical regions.					
31260951	1	50	theme	climacteric	131:141	arg1	Plantain					117:124	Plantain	117:124	Plantain	117:124	Plantain is a climacteric fruit having economic relevance in several tropical regions.					
31260951	5	51	theme	cooking	702:708	arg1	process					710:716	the cooking process	698:716	the cooking process	698:716	The microstructure results showed that the presence of fiber in the food matrix helped the reduction of the starch granule swelling in the cooking process.					
31260951	4	52	theme	Chemical	446:453	arg1	composition					455:465	Chemical composition	446:465	Chemical composition	446:465	Chemical composition, cooking quality, texture analysis, and microstructure of spaghetti formulations were analyzed.					
31260951	7	53	theme	whole	857:861	arg1	flour					879:883	the whole unripe plantain flour	853:883	the whole unripe plantain flour	853:883	Overall, the whole unripe plantain flour exhibited good potential for gluten-free spaghetti having highest content of fiber and lower starch digestion rates.					
31260951	2	54	theme	Unripe	204:209	arg1	plantain					211:218	Unripe plantain	204:218	Unripe plantain	204:218	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	2	54	theme	Unripe	204:209	arg1	source					238:243	an alternative source	223:243	an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction	223:322	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	6	55	theme	plantain	745:752	arg1	flour					754:758	whole plantain flour	739:758	whole plantain flour	739:758	Spaghetti made with whole plantain flour exhibited lower rapidly starch fraction, with increased resistant starch fractions.					
31260951	1	56	theme	tropical	186:193	arg1	regions					195:201	several tropical regions	178:201	several tropical regions	178:201	Plantain is a climacteric fruit having economic relevance in several tropical regions.					
31260951	0	57	theme	spaghetti	75:83	arg1	analysis					51:58	Physicochemical, microstructural and digestibility analysis	0:58	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.	0:115	Physicochemical, microstructural and digestibility analysis of gluten-free spaghetti of whole unripe plantain flour.					
31260951	2	58	theme	carbohydrates	261:273	arg1	plantain					211:218	Unripe plantain	204:218	Unripe plantain	204:218	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	2	58	theme	carbohydrates	261:273	arg1	source					238:243	an alternative source	223:243	an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction	223:322	Unripe plantain is an alternative source of indigestible carbohydrates (dietary fibre) and undigestible starch fraction.					
31260951	6	59	theme	whole	739:743	arg1	flour					754:758	whole plantain flour	739:758	whole plantain flour	739:758	Spaghetti made with whole plantain flour exhibited lower rapidly starch fraction, with increased resistant starch fractions.					
31260951	1	60	theme	economic	156:163	arg1	relevance					165:173	economic relevance	156:173	economic relevance	156:173	Plantain is a climacteric fruit having economic relevance in several tropical regions.					
30601085	0	0	theme	lymphatic	88:96	arg1	system					98:103	the lymphatic system	84:103	the lymphatic system	84:103	Engineering polymeric nanocapsules for an efficient drainage and biodistribution in the lymphatic system.					
30601085	7	1	from	accumulation	1162:1173	arg1	node					1198:1201	the popliteal lymph node	1178:1201	the popliteal lymph node	1178:1201	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	4	2	used	used	569:572	arg2	we					566:567	we	566:567	we	566:567	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	1	3	theme	immunological	172:184	arg1	response					186:193	the immunological response	168:193	the immunological response of antigens	168:205	Polymer-based nanocarriers have shown potential for enhancing the immunological response of antigens.					
30601085	9	4	theme	immune	1552:1557	arg1	response					1559:1566	the immune response	1548:1566	the immune response	1548:1566	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	3	5	theme	specific	507:514	arg1	cells					523:527	specific immune cells	507:527	specific immune cells	507:527	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	4	6	from	visualisation	675:687	arg1	levels					737:742	cellular/subcellular levels	716:742	cellular/subcellular levels	716:742	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	9	7	dep	tuning	1364:1369	arg1	properties					1391:1400	the physicochemical properties	1371:1400	the physicochemical properties	1371:1400	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	4	8	theme	powerful	576:583	arg1	technique					593:601	a powerful imaging technique	574:601	a powerful imaging technique	574:601	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	4	8	theme	powerful	576:583	arg1	microscopy					626:635	two-photon intravital microscopy	604:635	two-photon intravital microscopy	604:635	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	5	9	from	accumulation	820:831	arg1	critical					776:783	critical	776:783	critical	776:783	As expected, particle size was critical in the distribution and lymph node accumulation of all nanocapsules.					
30601085	4	10	theme	biological	692:701	arg1	processes					703:711	biological processes	692:711	biological processes at cellular/subcellular levels	692:742	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	8	11	theme	lumbar	1330:1335	arg1	node					1343:1346	the lumbar lymph node	1326:1346	the lumbar lymph node	1326:1346	In contrast, these anionic nanocapsules showed significant accumulation in the lumbar lymph node.					
30601085	3	12	theme	particle	343:350	arg1	size					352:355	particle size	343:355	particle size (≈100 versus 200 nm)	343:376	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	8	13	theme	anionic	1270:1276	arg1	nanocapsules					1278:1289	these anionic nanocapsules	1264:1289	these anionic nanocapsules	1264:1289	In contrast, these anionic nanocapsules showed significant accumulation in the lumbar lymph node.					
30601085	5	14	theme	nanocapsules	840:851	arg1	accumulation					820:831	the distribution and lymph node accumulation	788:831	the distribution and lymph node accumulation of all nanocapsules	788:851	As expected, particle size was critical in the distribution and lymph node accumulation of all nanocapsules.					
30601085	9	15	theme	lymphatic	1469:1477	arg1	uptake					1479:1484	their lymphatic uptake	1463:1484	their lymphatic uptake	1463:1484	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	4	16	from	damage	661:666	arg1	visualisation					675:687	the visualisation	671:687	the visualisation of biological processes at cellular/subcellular levels	671:742	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	4	17	theme	tissue	654:659	arg1	damage					661:666	tissue damage	654:666	tissue damage in the visualisation of biological processes at cellular/subcellular levels	654:742	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	3	18	theme	size	352:355	arg1	influence					330:338	the influence	326:338	the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics	326:545	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	9	19	contain	have	1517:1520	arg1	biodistribution					1490:1504	biodistribution	1490:1504	biodistribution	1490:1504	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	9	19	contain	have	1517:1520	arg2	implications					1532:1543	important implications	1522:1543	important implications	1522:1543	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	9	19	contain	have	1517:1520	arg1	uptake					1479:1484	their lymphatic uptake	1463:1484	their lymphatic uptake	1463:1484	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	9	20	theme	nanocapsules	1425:1436	arg1	composition					1406:1416	composition	1406:1416	composition	1406:1416	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	9	20	theme	nanocapsules	1425:1436	arg1	tuning					1364:1369	tuning	1364:1369	tuning	1364:1369	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	1	21	theme	antigens	198:205	arg1	response					186:193	the immunological response	168:193	the immunological response of antigens	168:205	Polymer-based nanocarriers have shown potential for enhancing the immunological response of antigens.					
30601085	0	22	theme	polymeric	12:20	arg1	nanocapsules					22:33	polymeric nanocapsules	12:33	polymeric nanocapsules for an efficient drainage and biodistribution	12:79	Engineering polymeric nanocapsules for an efficient drainage and biodistribution in the lymphatic system.					
30601085	7	23	theme	cationic	1100:1107	arg1	polyarginine					1136:1147	polyarginine	1136:1147	polyarginine	1136:1147	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	7	23	theme	cationic	1100:1107	arg1	chitosan					1123:1130	chitosan	1123:1130	chitosan	1123:1130	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	7	23	theme	cationic	1100:1107	arg1	nanocapsules					1109:1120	cationic nanocapsules	1100:1120	cationic nanocapsules (chitosan and polyarginine)	1100:1148	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	7	24	theme	different	1058:1066	arg1	shells					1078:1083	different polymeric shells	1058:1083	different polymeric shells	1058:1083	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	3	25	theme	nanocapsules	415:426	arg1	size					352:355	particle size	343:355	particle size (≈100 versus 200 nm)	343:376	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	25	theme	nanocapsules	415:426	arg1	composition					390:400	surface composition	382:400	surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan)	382:478	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	5	26	theme	node	815:818	arg1	accumulation					820:831	the distribution and lymph node accumulation	788:831	the distribution and lymph node accumulation of all nanocapsules	788:851	As expected, particle size was critical in the distribution and lymph node accumulation of all nanocapsules.					
30601085	6	27	theme	larger	980:985	arg1	size					987:990	a larger size	978:990	a larger size	978:990	Chitosan particles with a mean size below 100 nm accumulated significantly more in the popliteal lymph node than those with a larger size.					
30601085	7	28	theme	popliteal	1182:1190	arg1	node					1198:1201	the popliteal lymph node	1178:1201	the popliteal lymph node	1178:1201	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	7	29	with	nanocapsules	1040:1051	arg1	shells					1078:1083	different polymeric shells	1058:1083	different polymeric shells	1058:1083	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	9	30	theme	uptake	1479:1484	arg1	modulation					1449:1458	the modulation	1445:1458	the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response	1445:1566	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	1	31	theme	Polymer-based	106:118	arg1	nanocarriers					120:131	Polymer-based nanocarriers	106:131	Polymer-based nanocarriers	106:131	Polymer-based nanocarriers have shown potential for enhancing the immunological response of antigens.					
30601085	7	32	theme	lymph	1192:1196	arg1	node					1198:1201	the popliteal lymph node	1178:1201	the popliteal lymph node	1178:1201	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	6	33	theme	mean	880:883	arg1	size					885:888	a mean size	878:888	a mean size below 100 nm	878:901	Chitosan particles with a mean size below 100 nm accumulated significantly more in the popliteal lymph node than those with a larger size.					
30601085	7	34	theme	nanocapsules	1040:1051	arg1	analysis					1021:1028	a comparative analysis	1007:1028	a comparative analysis of 100 nm nanocapsules with different polymeric shells	1007:1083	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	4	35	theme	intravital	615:624	arg1	technique					593:601	a powerful imaging technique	574:601	a powerful imaging technique	574:601	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	4	35	theme	intravital	615:624	arg1	microscopy					626:635	two-photon intravital microscopy	604:635	two-photon intravital microscopy	604:635	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	0	36	theme	efficient	42:50	arg1	drainage					52:59	an efficient drainage	39:59	an efficient drainage	39:59	Engineering polymeric nanocapsules for an efficient drainage and biodistribution in the lymphatic system.					
30601085	9	37	theme	physicochemical	1375:1389	arg1	properties					1391:1400	the physicochemical properties	1371:1400	the physicochemical properties	1371:1400	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	7	38	theme	polymeric	1068:1076	arg1	shells					1078:1083	different polymeric shells	1058:1083	different polymeric shells	1058:1083	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	3	39	dep	nanocapsules	415:426	arg1	chitosan					429:436	chitosan	429:436	chitosan	429:436	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	39	dep	nanocapsules	415:426	arg1	nanocapsules					415:426	polymeric nanocapsules	405:426	polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan)	405:478	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	39	dep	nanocapsules	415:426	arg1	carboxymethyl-β-glucan					456:477	carboxymethyl-β-glucan	456:477	carboxymethyl-β-glucan	456:477	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	39	dep	nanocapsules	415:426	arg1	polyarginine					439:450	polyarginine	439:450	polyarginine	439:450	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	7	40	theme	higher	1155:1160	arg1	accumulation					1162:1173	higher accumulation	1155:1173	higher accumulation in the popliteal lymph node	1155:1201	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	3	41	theme	polymeric	405:413	arg1	chitosan					429:436	chitosan	429:436	chitosan	429:436	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	41	theme	polymeric	405:413	arg1	nanocapsules					415:426	polymeric nanocapsules	405:426	polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan)	405:478	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	41	theme	polymeric	405:413	arg1	carboxymethyl-β-glucan					456:477	carboxymethyl-β-glucan	456:477	carboxymethyl-β-glucan	456:477	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	41	theme	polymeric	405:413	arg1	polyarginine					439:450	polyarginine	439:450	polyarginine	439:450	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	7	42	dep	nanocapsules	1109:1120	arg1	polyarginine					1136:1147	polyarginine	1136:1147	polyarginine	1136:1147	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	7	42	dep	nanocapsules	1109:1120	arg1	chitosan					1123:1130	chitosan	1123:1130	chitosan	1123:1130	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	7	42	dep	nanocapsules	1109:1120	arg1	nanocapsules					1109:1120	cationic nanocapsules	1100:1120	cationic nanocapsules (chitosan and polyarginine)	1100:1148	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	9	43	theme	important	1522:1530	arg1	implications					1532:1543	important implications	1522:1543	important implications	1522:1543	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	7	44	theme	anionic	1212:1218	arg1	ones					1220:1223	the anionic ones	1208:1223	the anionic ones (carboxymethyl-β-glucan)	1208:1248	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	7	44	theme	anionic	1212:1218	arg1	carboxymethyl-β-glucan					1226:1247	carboxymethyl-β-glucan	1226:1247	carboxymethyl-β-glucan	1226:1247	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	3	45	theme	surface	382:388	arg1	composition					390:400	surface composition	382:400	surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan)	382:478	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	46	theme	composition	390:400	arg1	influence					330:338	the influence	326:338	the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics	326:545	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	6	47	theme	lymph	951:955	arg1	node					957:960	the popliteal lymph node	937:960	the popliteal lymph node	937:960	Chitosan particles with a mean size below 100 nm accumulated significantly more in the popliteal lymph node than those with a larger size.					
30601085	3	48	dep	size	352:355	arg1	200 nm					370:375	200 nm	370:375	200 nm	370:375	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	48	dep	size	352:355	arg1	≈100					358:361	≈100	358:361	≈100	358:361	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	6	49	theme	Chitosan	854:861	arg1	particles					863:871	Chitosan particles	854:871	Chitosan particles with a mean size below 100 nm	854:901	Chitosan particles with a mean size below 100 nm accumulated significantly more in the popliteal lymph node than those with a larger size.					
30601085	5	50	from	critical	776:783	arg1	accumulation					820:831	the distribution and lymph node accumulation	788:831	the distribution and lymph node accumulation of all nanocapsules	788:851	As expected, particle size was critical in the distribution and lymph node accumulation of all nanocapsules.					
30601085	4	51	theme	processes	703:711	arg1	visualisation					675:687	the visualisation	671:687	the visualisation of biological processes at cellular/subcellular levels	671:742	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	4	52	from	levels	737:742	arg1	visualisation					675:687	the visualisation	671:687	the visualisation of biological processes at cellular/subcellular levels	671:742	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	4	52	from	levels	737:742	arg1	processes					703:711	biological processes	692:711	biological processes at cellular/subcellular levels	692:742	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	6	53	theme	popliteal	941:949	arg1	node					957:960	the popliteal lymph node	937:960	the popliteal lymph node	937:960	Chitosan particles with a mean size below 100 nm accumulated significantly more in the popliteal lymph node than those with a larger size.					
30601085	7	54	theme	100 nm	1033:1038	arg1	nanocapsules					1040:1051	100 nm nanocapsules	1033:1051	100 nm nanocapsules with different polymeric shells	1033:1083	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	2	55	theme	key	221:223	arg1	drivers					225:231	the key drivers	217:231	the key drivers for this response	217:249	However, the key drivers for this response have not been fully elucidated.					
30601085	7	56	theme	comparative	1009:1019	arg1	analysis					1021:1028	a comparative analysis	1007:1028	a comparative analysis of 100 nm nanocapsules with different polymeric shells	1007:1083	Additionally, a comparative analysis of 100 nm nanocapsules with different polymeric shells indicated that cationic nanocapsules (chitosan and polyarginine) show higher accumulation in the popliteal lymph node than the anionic ones (carboxymethyl-β-glucan).					
30601085	8	57	theme	lymph	1337:1341	arg1	node					1343:1346	the lumbar lymph node	1326:1346	the lumbar lymph node	1326:1346	In contrast, these anionic nanocapsules showed significant accumulation in the lumbar lymph node.					
30601085	3	58	theme	work	305:308	arg1	objective					287:295	The objective	283:295	The objective of this work	283:308	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	3	59	from	influence	330:338	arg1	ability					489:495	their ability to target specific immune cells in the lymphatics	483:545	their ability to target specific immune cells in the lymphatics	483:545	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	8	60	from	accumulation	1310:1321	arg1	node					1343:1346	the lumbar lymph node	1326:1346	the lumbar lymph node	1326:1346	In contrast, these anionic nanocapsules showed significant accumulation in the lumbar lymph node.					
30601085	4	61	theme	cellular/subcellular	716:735	arg1	levels					737:742	cellular/subcellular levels	716:742	cellular/subcellular levels	716:742	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	4	62	theme	imaging	585:591	arg1	technique					593:601	a powerful imaging technique	574:601	a powerful imaging technique	574:601	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	4	62	theme	imaging	585:591	arg1	microscopy					626:635	two-photon intravital microscopy	604:635	two-photon intravital microscopy	604:635	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	5	63	theme	particle	758:765	arg1	size					767:770	particle size	758:770	particle size	758:770	As expected, particle size was critical in the distribution and lymph node accumulation of all nanocapsules.					
30601085	8	64	theme	significant	1298:1308	arg1	accumulation					1310:1321	significant accumulation	1298:1321	significant accumulation in the lumbar lymph node	1298:1346	In contrast, these anionic nanocapsules showed significant accumulation in the lumbar lymph node.					
30601085	3	65	theme	immune	516:521	arg1	cells					523:527	specific immune cells	507:527	specific immune cells	507:527	The objective of this work was to evaluate the influence of particle size (≈100 versus 200 nm) and surface composition of polymeric nanocapsules (chitosan, polyarginine and carboxymethyl-β-glucan) on their ability to target specific immune cells in the lymphatics.					
30601085	6	66	with	particles	863:871	arg1	size					885:888	a mean size	878:888	a mean size below 100 nm	878:901	Chitosan particles with a mean size below 100 nm accumulated significantly more in the popliteal lymph node than those with a larger size.					
30601085	4	67	theme	two-photon	604:613	arg1	technique					593:601	a powerful imaging technique	574:601	a powerful imaging technique	574:601	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	4	67	theme	two-photon	604:613	arg1	microscopy					626:635	two-photon intravital microscopy	604:635	two-photon intravital microscopy	604:635	For this purpose, we used a powerful imaging technique, two-photon intravital microscopy, which minimises tissue damage in the visualisation of biological processes at cellular/subcellular levels.					
30601085	5	68	theme	distribution	792:803	arg1	accumulation					820:831	the distribution and lymph node accumulation	788:831	the distribution and lymph node accumulation of all nanocapsules	788:851	As expected, particle size was critical in the distribution and lymph node accumulation of all nanocapsules.					
30601085	9	69	theme	biodistribution	1490:1504	arg1	modulation					1449:1458	the modulation	1445:1458	the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response	1445:1566	In conclusion, tuning the physicochemical properties and composition of the nanocapsules allows the modulation of their lymphatic uptake and biodistribution, which may have important implications in the immune response.					
30601085	5	70	theme	lymph	809:813	arg1	node					815:818	lymph node	809:818	lymph node	809:818	As expected, particle size was critical in the distribution and lymph node accumulation of all nanocapsules.					
29940003	9	0	theme	conditions	1992:2001	arg1	optimization					1959:1970	optimization	1959:1970	optimization	1959:1970	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	9	0	theme	conditions	1992:2001	arg1	design					1948:1953	design	1948:1953	design	1948:1953	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	8	1	theme	initial	1776:1782	arg1	densities					1789:1797	the initial cell densities	1772:1797	the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8	1772:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	2	2	theme	simultaneous	367:378	arg1	fermentation					380:391	simultaneous fermentation	367:391	simultaneous fermentation of cellobiose and xylose	367:416	The present study reports a new approach for simultaneous fermentation of cellobiose and xylose by using the co-culture consisting of recombinant Saccharomyces cerevisiae specialist strains.					
29940003	6	3	theme	inhibition	1299:1308	arg1	concentration					1329:1341	initial substrate concentration	1311:1341	initial substrate concentration	1311:1341	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	6	3	theme	inhibition	1299:1308	arg1	size					1356:1359	inoculum size	1347:1359	inoculum size	1347:1359	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	6	3	theme	inhibition	1299:1308	arg1	effects					1280:1286	the effects	1276:1286	the effects of product inhibition	1276:1308	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	3	4	theme	culture	606:612	arg1	system					614:619	the single culture system	595:619	the single culture system	595:619	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	7	5	theme	batch	1572:1576	arg1	fermentation					1578:1589	batch fermentation	1572:1589	batch fermentation of cellobiose, xylose and their mixtures	1572:1630	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	9	6	dep	design	1948:1953	arg1	the					1944:1946	the	1944:1946	the	1944:1946	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	8	7	theme	g	1844:1844	arg1	weight					1855:1860	EJ2 and 0.9 g dry cell weight /L	1832:1863	EJ2 and 0.9 g dry cell weight /L SR8	1832:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	7	8	theme	xylose	1606:1611	arg1	fermentation					1578:1589	batch fermentation	1572:1589	batch fermentation of cellobiose, xylose and their mixtures	1572:1630	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	9	9	theme	cellobiose	2046:2055	arg1	fermentation					2030:2041	simultaneous fermentation	2017:2041	simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass	2017:2215	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	8	10	theme	cell	1850:1853	arg1	weight					1855:1860	EJ2 and 0.9 g dry cell weight /L	1832:1863	EJ2 and 0.9 g dry cell weight /L SR8	1832:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	9	11	theme	xylose	2061:2066	arg1	fermentation					2030:2041	simultaneous fermentation	2017:2041	simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass	2017:2215	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	7	12	theme	model	1527:1531	arg1	predictions					1533:1543	model predictions	1527:1543	model predictions	1527:1543	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	9	13	theme	improved	2073:2080	arg1	productivity					2090:2101	improved ethanol productivity	2073:2101	improved ethanol productivity	2073:2101	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	5	14	theme	kinetic	943:949	arg1	modeling					951:958	kinetic modeling	943:958	kinetic modeling	943:958	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	6	15	theme	inoculum	1347:1354	arg1	size					1356:1359	inoculum size	1347:1359	inoculum size	1347:1359	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	6	15	theme	inoculum	1347:1354	arg1	effects					1280:1286	the effects	1276:1286	the effects of product inhibition	1276:1308	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	5	16	with	sugars	1069:1074	arg1	biomass					1092:1098	adjustable biomass	1081:1098	adjustable biomass of each specialist strain	1081:1124	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	4	17	theme	recombinant	800:810	arg1	strain					847:852	the recombinant cellobiose-consuming S. cerevisiae strain EJ2	796:856	the recombinant cellobiose-consuming S. cerevisiae strain EJ2	796:856	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	1	18	theme	cellulosic	144:153	arg1	sugars					155:160	cellulosic sugars	144:160	cellulosic sugars	144:160	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	6	19	theme	initial	1311:1317	arg1	concentration					1329:1341	initial substrate concentration	1311:1341	initial substrate concentration	1311:1341	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	6	19	theme	initial	1311:1317	arg1	effects					1280:1286	the effects	1276:1286	the effects of product inhibition	1276:1308	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	4	20	theme	fermentation	771:782	arg1	kinetics					784:791	fermentation kinetics	771:791	fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture	771:921	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	0	21	theme	Saccharomyces	73:85	arg1	cerevisiae					87:96	recombinant Saccharomyces cerevisiae	61:96	recombinant Saccharomyces cerevisiae	61:96	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.					
29940003	6	22	theme	product	1291:1297	arg1	inhibition					1299:1308	product inhibition	1291:1308	product inhibition	1291:1308	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	5	23	theme	sugars	1069:1074	arg1	fermentation					1047:1058	simultaneous fermentation	1034:1058	simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain	1034:1124	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	8	24	theme	model	1668:1672	arg1	prediction					1674:1683	model prediction	1668:1683	model prediction	1668:1683	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	9	25	theme	renewable	2159:2167	arg1	production					2177:2186	robust and efficient renewable biofuel production	2138:2186	production	2177:2186	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	4	26	theme	S.	876:877	arg1	SR8					897:899	xylose-consuming S. cerevisiae strain SR8	859:899	xylose-consuming S. cerevisiae strain SR8	859:899	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	0	27	theme	modeling	110:117	arg1	culture					50:56	mixed culture	44:56	mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling	44:117	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.					
29940003	5	28	theme	adjustable	1081:1090	arg1	biomass					1092:1098	adjustable biomass	1081:1098	adjustable biomass of each specialist strain	1081:1124	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	2	29	theme	specialist	493:502	arg1	strains					504:510	recombinant Saccharomyces cerevisiae specialist strains	456:510	recombinant Saccharomyces cerevisiae specialist strains	456:510	The present study reports a new approach for simultaneous fermentation of cellobiose and xylose by using the co-culture consisting of recombinant Saccharomyces cerevisiae specialist strains.					
29940003	4	30	theme	S.	833:834	arg1	strain					847:852	the recombinant cellobiose-consuming S. cerevisiae strain EJ2	796:856	the recombinant cellobiose-consuming S. cerevisiae strain EJ2	796:856	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	2	31	theme	xylose	411:416	arg1	fermentation					380:391	simultaneous fermentation	367:391	simultaneous fermentation of cellobiose and xylose	367:416	The present study reports a new approach for simultaneous fermentation of cellobiose and xylose by using the co-culture consisting of recombinant Saccharomyces cerevisiae specialist strains.					
29940003	5	32	theme	simultaneous	1034:1045	arg1	fermentation					1047:1058	simultaneous fermentation	1034:1058	simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain	1034:1124	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	7	33	theme	substrate	1468:1476	arg1	conditions					1478:1487	different substrate conditions	1458:1487	different substrate conditions	1458:1487	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	9	34	theme	lignocellulosic	2193:2207	arg1	biomass					2209:2215	lignocellulosic biomass	2193:2215	lignocellulosic biomass	2193:2215	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	4	35	theme	strain	847:852	arg1	kinetics					784:791	fermentation kinetics	771:791	fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture	771:921	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	0	36	theme	cellobiose	19:28	arg1	Co-fermentation					0:14	Co-fermentation	0:14	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.	0:118	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.					
29940003	1	37	theme	sugars	155:160	arg1	conversion					130:139	Efficient conversion	120:139	Efficient conversion of cellulosic sugars in cellulosic hydrolysates	120:187	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	8	38	theme	60	1718:1719	arg1	g/L					1721:1723	g/L	1721:1723	g/L	1721:1723	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	0	39	theme	xylose	34:39	arg1	Co-fermentation					0:14	Co-fermentation	0:14	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.	0:118	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.					
29940003	7	40	theme	fermentation	1427:1438	arg1	experiments					1440:1450	independent fermentation experiments	1415:1450	independent fermentation experiments under different substrate conditions	1415:1487	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	8	41	theme	cellobiose	1725:1734	arg1	co-fermentation					1699:1713	simultaneous co-fermentation	1686:1713	simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose	1686:1752	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	0	42	theme	mixed	44:48	arg1	culture					50:56	mixed culture	44:56	mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling	44:117	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.					
29940003	5	43	theme	substrate	1142:1150	arg1	concentrations					1152:1165	different substrate concentrations	1132:1165	different substrate concentrations	1132:1165	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	7	44	from	experiments	1440:1450	arg1	results					1402:1408	results	1402:1408	results from independent fermentation experiments under different substrate conditions	1402:1487	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	6	45	theme	pure	1239:1242	arg1	models					1252:1257	the pure culture models	1235:1257	the pure culture models	1235:1257	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	8	46	theme	20	1740:1741	arg1	g/L					1743:1745	g/L	1743:1745	g/L	1743:1745	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	4	47	theme	strain	890:895	arg1	SR8					897:899	xylose-consuming S. cerevisiae strain SR8	859:899	xylose-consuming S. cerevisiae strain SR8	859:899	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	2	48	theme	new	350:352	arg1	approach					354:361	a new approach	348:361	a new approach for simultaneous fermentation of cellobiose and xylose	348:416	The present study reports a new approach for simultaneous fermentation of cellobiose and xylose by using the co-culture consisting of recombinant Saccharomyces cerevisiae specialist strains.					
29940003	8	49	theme	xylose	1747:1752	arg1	co-fermentation					1699:1713	simultaneous co-fermentation	1686:1713	simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose	1686:1752	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	3	50	theme	biofuel	726:732	arg1	production					734:743	robust and cost-effective biofuel production	700:743	robust and cost-effective biofuel production	700:743	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	3	51	theme	modularity	572:581	arg1	advantage					559:567	competitive advantage	547:567	competitive advantage of modularity compared to the single culture system	547:619	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	8	52	theme	g	1807:1807	arg1	weight					1818:1823	0.45 g dry cell weight /L	1802:1826	0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8	1802:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	5	53	theme	specialist	1108:1117	arg1	strain					1119:1124	each specialist strain	1103:1124	each specialist strain	1103:1124	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	9	54	theme	co-culture	1981:1990	arg1	conditions					1992:2001	yeast co-culture conditions	1975:2001	yeast co-culture conditions	1975:2001	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	8	55	theme	cell	1813:1816	arg1	weight					1818:1823	0.45 g dry cell weight /L	1802:1826	0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8	1802:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	7	56	theme	mixtures	1623:1630	arg1	fermentation					1578:1589	batch fermentation	1572:1589	batch fermentation of cellobiose, xylose and their mixtures	1572:1630	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	7	57	theme	model	1366:1370	arg1	simulations					1372:1382	The model simulations	1362:1382	The model simulations	1362:1382	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	3	58	theme	single	599:604	arg1	system					614:619	the single culture system	595:619	the single culture system	595:619	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	7	59	theme	cellobiose	1594:1603	arg1	fermentation					1578:1589	batch fermentation	1572:1589	batch fermentation of cellobiose, xylose and their mixtures	1572:1630	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	9	60	theme	simultaneous	2017:2028	arg1	fermentation					2030:2041	simultaneous fermentation	2017:2041	simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass	2017:2215	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	8	61	theme	cell	1784:1787	arg1	densities					1789:1797	the initial cell densities	1772:1797	the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8	1772:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	2	62	theme	present	326:332	arg1	study					334:338	The present study	322:338	The present study	322:338	The present study reports a new approach for simultaneous fermentation of cellobiose and xylose by using the co-culture consisting of recombinant Saccharomyces cerevisiae specialist strains.					
29940003	8	63	theme	dry	1846:1848	arg1	weight					1855:1860	EJ2 and 0.9 g dry cell weight /L	1832:1863	EJ2 and 0.9 g dry cell weight /L SR8	1832:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	1	64	theme	viable	219:224	arg1	production					226:235	economically viable production	206:235	economically viable production of biofuels from lignocellulosic biomass	206:276	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	2	65	theme	cellobiose	396:405	arg1	fermentation					380:391	simultaneous fermentation	367:391	simultaneous fermentation of cellobiose and xylose	367:416	The present study reports a new approach for simultaneous fermentation of cellobiose and xylose by using the co-culture consisting of recombinant Saccharomyces cerevisiae specialist strains.					
29940003	7	66	theme	experimental	1549:1560	arg1	data					1562:1565	experimental data	1549:1565	experimental data from batch fermentation of cellobiose, xylose and their mixtures	1549:1630	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	8	67	theme	weight	1855:1860	arg1	SR8					1865:1867	EJ2 and 0.9 g dry cell weight /L SR8	1832:1867	EJ2 and 0.9 g dry cell weight /L SR8	1832:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	9	68	used	used	1930:1933	arg2	modeling					1912:1919	the kinetic modeling	1900:1919	the kinetic modeling	1900:1919	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	9	69	theme	ethanol	2082:2088	arg1	productivity					2090:2101	improved ethanol productivity	2073:2101	improved ethanol productivity	2073:2101	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	1	70	from	biomass	270:276	arg1	production					226:235	economically viable production	206:235	economically viable production of biofuels from lignocellulosic biomass	206:276	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	1	70	from	biomass	270:276	arg1	biofuels					240:247	biofuels	240:247	biofuels from lignocellulosic biomass	240:276	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	1	71	theme	Efficient	120:128	arg1	conversion					130:139	Efficient conversion	120:139	Efficient conversion of cellulosic sugars in cellulosic hydrolysates	120:187	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	3	72	theme	feedstock	667:675	arg1	composition					677:687	feedstock composition	667:687	feedstock composition	667:687	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	1	73	from	production	226:235	arg1	biomass					270:276	lignocellulosic biomass	254:276	lignocellulosic biomass	254:276	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	5	74	theme	co-culture	1012:1021	arg1	system					1023:1028	the co-culture system	1008:1028	the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain	1008:1124	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	6	75	theme	substrate	1319:1327	arg1	concentration					1329:1341	initial substrate concentration	1311:1341	initial substrate concentration	1311:1341	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	6	75	theme	substrate	1319:1327	arg1	effects					1280:1286	the effects	1276:1286	the effects of product inhibition	1276:1308	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	0	76	theme	recombinant	61:71	arg1	cerevisiae					87:96	recombinant Saccharomyces cerevisiae	61:96	recombinant Saccharomyces cerevisiae	61:96	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.					
29940003	4	77	dep	S.	876:877	arg1	cerevisiae					879:888	cerevisiae	879:888	cerevisiae	879:888	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	7	78	theme	good	1494:1497	arg1	agreement					1499:1507	good agreement	1494:1507	good agreement	1494:1507	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	0	79	theme	cerevisiae	87:96	arg1	culture					50:56	mixed culture	44:56	mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling	44:117	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.					
29940003	1	80	theme	cellulosic	165:174	arg1	hydrolysates					176:187	cellulosic hydrolysates	165:187	cellulosic hydrolysates	165:187	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	7	81	from	fermentation	1578:1589	arg1	data					1562:1565	experimental data	1549:1565	experimental data from batch fermentation of cellobiose, xylose and their mixtures	1549:1630	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	7	81	from	fermentation	1578:1589	arg1	predictions					1533:1543	model predictions	1527:1543	model predictions	1527:1543	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	9	82	theme	robust	2138:2143	arg1	production					2177:2186	robust and efficient renewable biofuel production	2138:2186	production	2177:2186	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	0	83	theme	kinetic	102:108	arg1	modeling					110:117	kinetic modeling	102:117	kinetic modeling	102:117	Co-fermentation of cellobiose and xylose by mixed culture of recombinant Saccharomyces cerevisiae and kinetic modeling.					
29940003	5	84	theme	mixed	1063:1067	arg1	sugars					1069:1074	mixed sugars	1063:1074	mixed sugars with adjustable biomass of each specialist strain	1063:1124	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	8	85	theme	prediction	1674:1683	arg1	guidance					1656:1663	the guidance	1652:1663	the guidance of model prediction	1652:1683	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	9	86	theme	efficient	2149:2157	arg1	production					2177:2186	robust and efficient renewable biofuel production	2138:2186	production	2177:2186	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	3	87	from	fluctuations	651:662	arg1	composition					677:687	feedstock composition	667:687	feedstock composition	667:687	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	3	88	with	deal	641:644	arg1	fluctuations					651:662	fluctuations	651:662	fluctuations in feedstock composition	651:687	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	6	89	theme	co-culture	1194:1203	arg1	system					1205:1210	the co-culture system	1190:1210	the co-culture system	1190:1210	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	9	90	theme	kinetic	1904:1910	arg1	modeling					1912:1919	the kinetic modeling	1900:1919	the kinetic modeling	1900:1919	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	8	91	theme	simultaneous	1686:1697	arg1	co-fermentation					1699:1713	simultaneous co-fermentation	1686:1713	simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose	1686:1752	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	9	92	theme	biofuel	2169:2175	arg1	production					2177:2186	robust and efficient renewable biofuel production	2138:2186	production	2177:2186	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	4	93	theme	xylose-consuming	859:874	arg1	SR8					897:899	xylose-consuming S. cerevisiae strain SR8	859:899	xylose-consuming S. cerevisiae strain SR8	859:899	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	9	94	from	biomass	2209:2215	arg1	production					2177:2186	robust and efficient renewable biofuel production	2138:2186	production	2177:2186	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	7	95	theme	different	1458:1466	arg1	conditions					1478:1487	different substrate conditions	1458:1487	different substrate conditions	1458:1487	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	2	96	theme	cerevisiae	482:491	arg1	strains					504:510	recombinant Saccharomyces cerevisiae specialist strains	456:510	recombinant Saccharomyces cerevisiae specialist strains	456:510	The present study reports a new approach for simultaneous fermentation of cellobiose and xylose by using the co-culture consisting of recombinant Saccharomyces cerevisiae specialist strains.					
29940003	9	97	with	fermentation	2030:2041	arg1	productivity					2090:2101	improved ethanol productivity	2073:2101	improved ethanol productivity	2073:2101	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	4	98	theme	cellobiose-consuming	812:831	arg1	strain					847:852	the recombinant cellobiose-consuming S. cerevisiae strain EJ2	796:856	the recombinant cellobiose-consuming S. cerevisiae strain EJ2	796:856	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	1	99	theme	critical	302:309	arg1	challenge					311:319	a critical challenge	300:319	a critical challenge	300:319	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	1	100	from	conversion	130:139	arg1	hydrolysates					176:187	cellulosic hydrolysates	165:187	cellulosic hydrolysates	165:187	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	6	101	theme	kinetic	1172:1178	arg1	model					1180:1184	The kinetic model	1168:1184	The kinetic model for the co-culture system	1168:1210	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	2	102	theme	recombinant	456:466	arg1	strains					504:510	recombinant Saccharomyces cerevisiae specialist strains	456:510	recombinant Saccharomyces cerevisiae specialist strains	456:510	The present study reports a new approach for simultaneous fermentation of cellobiose and xylose by using the co-culture consisting of recombinant Saccharomyces cerevisiae specialist strains.					
29940003	8	103	theme	g/L	1721:1723	arg1	cellobiose					1725:1734	60 g/L cellobiose	1718:1734	60 g/L cellobiose	1718:1734	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	3	104	theme	robust	700:705	arg1	production					734:743	robust and cost-effective biofuel production	700:743	robust and cost-effective biofuel production	700:743	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	4	105	theme	cerevisiae	836:845	arg1	strain					847:852	the recombinant cellobiose-consuming S. cerevisiae strain EJ2	796:856	the recombinant cellobiose-consuming S. cerevisiae strain EJ2	796:856	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	3	106	theme	co-culture	517:526	arg1	system					528:533	The co-culture system	513:533	The co-culture system	513:533	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	3	106	theme	co-culture	517:526	arg1	tuned					632:636	tuned	632:636	tuned	632:636	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	5	107	theme	strain	1119:1124	arg1	biomass					1092:1098	adjustable biomass	1081:1098	adjustable biomass of each specialist strain	1081:1124	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	7	108	theme	independent	1415:1425	arg1	experiments					1440:1450	independent fermentation experiments	1415:1450	independent fermentation experiments under different substrate conditions	1415:1487	The model simulations were validated by results from independent fermentation experiments under different substrate conditions, and good agreement was found between model predictions and experimental data from batch fermentation of cellobiose, xylose and their mixtures.					
29940003	5	109	theme	different	1132:1140	arg1	concentrations					1152:1165	different substrate concentrations	1132:1165	different substrate concentrations	1132:1165	The motivation for kinetic modeling was to provide guidance and prediction of using the co-culture system for simultaneous fermentation of mixed sugars with adjustable biomass of each specialist strain under different substrate concentrations.					
29940003	6	110	theme	culture	1244:1250	arg1	models					1252:1257	the pure culture models	1235:1257	the pure culture models	1235:1257	The kinetic model for the co-culture system was developed based on the pure culture models and incorporated the effects of product inhibition, initial substrate concentration and inoculum size.					
29940003	8	111	theme	g/L	1743:1745	arg1	xylose					1747:1752	20 g/L xylose	1740:1752	20 g/L xylose	1740:1752	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	4	112	theme	co-culture	912:921	arg1	kinetics					784:791	fermentation kinetics	771:791	fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture	771:921	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	3	113	theme	competitive	547:557	arg1	advantage					559:567	competitive advantage	547:567	competitive advantage of modularity compared to the single culture system	547:619	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	3	114	theme	cost-effective	711:724	arg1	production					734:743	robust and cost-effective biofuel production	700:743	robust and cost-effective biofuel production	700:743	The co-culture system can provide competitive advantage of modularity compared to the single culture system and can be tuned to deal with fluctuations in feedstock composition to achieve robust and cost-effective biofuel production.					
29940003	8	115	theme	dry	1809:1811	arg1	weight					1818:1823	0.45 g dry cell weight /L	1802:1826	0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8	1802:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
29940003	1	116	theme	lignocellulosic	254:268	arg1	biomass					270:276	lignocellulosic biomass	254:276	lignocellulosic biomass	254:276	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	1	117	theme	biofuels	240:247	arg1	production					226:235	economically viable production	206:235	economically viable production of biofuels from lignocellulosic biomass	206:276	Efficient conversion of cellulosic sugars in cellulosic hydrolysates is important for economically viable production of biofuels from lignocellulosic biomass, but the goal remains a critical challenge.					
29940003	9	118	theme	yeast	1975:1979	arg1	conditions					1992:2001	yeast co-culture conditions	1975:2001	yeast co-culture conditions	1975:2001	The results demonstrated that the kinetic modeling could be used to guide the design and optimization of yeast co-culture conditions for achieving simultaneous fermentation of cellobiose and xylose with improved ethanol productivity, which is critically important for robust and efficient renewable biofuel production from lignocellulosic biomass.					
29940003	4	119	theme	SR8	897:899	arg1	kinetics					784:791	fermentation kinetics	771:791	fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture	771:921	This study characterized fermentation kinetics of the recombinant cellobiose-consuming S. cerevisiae strain EJ2, xylose-consuming S. cerevisiae strain SR8, and their co-culture.					
29940003	8	120	theme	weight	1818:1823	arg1	densities					1789:1797	the initial cell densities	1772:1797	the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8	1772:1867	Additionally, with the guidance of model prediction, simultaneous co-fermentation of 60 g/L cellobiose and 20 g/L xylose was achieved with the initial cell densities of 0.45 g dry cell weight /L for EJ2 and 0.9 g dry cell weight /L SR8.					
31857183	5	0	theme	hydrogels	984:992	arg1	system					994:999	hydrogels system	984:999	hydrogels system with nanohydroxyapatite	984:1023	A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite.					
31857183	1	1	theme	bacteriophages-loaded	187:207	arg1	hydrogel					237:244	bacteriophages-loaded alginate-nanohydroxyapatite hydrogel	187:244	bacteriophages-loaded alginate-nanohydroxyapatite hydrogel	187:244	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	1	2	theme	infection	324:332	arg1	prevention					334:343	infection prevention	324:343	infection prevention	324:343	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	5	3	theme	system	994:999	arg1	implantation					968:979	the implantation	964:979	the implantation of hydrogels system with nanohydroxyapatite	964:1023	A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite.					
31857183	0	4	from	bacteriophages	13:26	arg1	hydrogel					59:66	alginate-nanohydroxyapatite hydrogel	31:66	alginate-nanohydroxyapatite hydrogel	31:66	Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections.					
31857183	1	5	theme	alginate-nanohydroxyapatite	209:235	arg1	hydrogel					237:244	bacteriophages-loaded alginate-nanohydroxyapatite hydrogel	187:244	bacteriophages-loaded alginate-nanohydroxyapatite hydrogel	187:244	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	0	6	theme	implant-associated	117:134	arg1	infections					136:145	orthopedic implant-associated infections	106:145	orthopedic implant-associated infections	106:145	Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections.					
31857183	6	7	theme	surrounding	1183:1193	arg1	faecalis					1174:1181	multidrug-resistant E. faecalis surrounding	1151:1193	multidrug-resistant E. faecalis surrounding	1151:1193	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	7	8	theme	local	1232:1236	arg1	approach					1277:1284	a promising approach	1265:1284	a promising approach to prevent and control bacterial contamination during implantation and bone integration	1265:1372	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	7	8	theme	local	1232:1236	arg1	approach					1247:1254	This new local delivery approach	1223:1254	This new local delivery approach	1223:1254	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	0	9	theme	orthopedic	106:115	arg1	infections					136:145	orthopedic implant-associated infections	106:145	orthopedic implant-associated infections	106:145	Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections.					
31857183	6	10	theme	faecalis	1174:1181	arg1	attachment					1120:1129	attachment	1120:1129	attachment	1120:1129	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	6	10	theme	faecalis	1174:1181	arg1	colonization					1135:1146	colonization	1135:1146	colonization	1135:1146	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	6	11	theme	bacteriophages-loaded	1034:1054	arg1	hydrogel					1056:1063	bacteriophages-loaded hydrogel	1034:1063	bacteriophages-loaded hydrogel	1034:1063	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	3	12	theme	pH.	661:663	arg1	response					677:684	environmental pH. Good tissue response	647:684	environmental pH. Good tissue response	647:684	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	6	13	theme	E.	1171:1172	arg1	faecalis					1174:1181	multidrug-resistant E. faecalis surrounding	1151:1193	multidrug-resistant E. faecalis surrounding	1151:1193	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	7	14	theme	delivery	1238:1245	arg1	approach					1277:1284	a promising approach	1265:1284	a promising approach to prevent and control bacterial contamination during implantation and bone integration	1265:1372	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	7	14	theme	delivery	1238:1245	arg1	approach					1247:1254	This new local delivery approach	1223:1254	This new local delivery approach	1223:1254	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	6	15	theme	multidrug-resistant	1151:1169	arg1	faecalis					1174:1181	multidrug-resistant E. faecalis surrounding	1151:1193	multidrug-resistant E. faecalis surrounding	1151:1193	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	2	16	theme	chemical	498:505	arg1	composition					507:517	chemical composition	498:517	chemical composition	498:517	Bacteriophages were efficiently encapsulated, without jeopardizing phage viability and functionality, nor affecting hydrogel morphology and chemical composition.					
31857183	7	17	theme	new	1228:1230	arg1	approach					1277:1284	a promising approach	1265:1284	a promising approach to prevent and control bacterial contamination during implantation and bone integration	1265:1372	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	7	17	theme	new	1228:1230	arg1	approach					1247:1254	This new local delivery approach	1223:1254	This new local delivery approach	1223:1254	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	3	18	theme	bacteriophages-loaded	729:749	arg1	hydrogels					751:759	bacteriophages-loaded hydrogels	729:759	bacteriophages-loaded hydrogels	729:759	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	0	19	theme	Encapsulated	0:11	arg1	bacteriophages					13:26	Encapsulated bacteriophages	0:26	Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system	0:93	Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections.					
31857183	3	20	theme	hydrogels	751:759	arg1	implantation					713:724	the implantation	709:724	the implantation of bacteriophages-loaded hydrogels	709:759	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	3	21	theme	swelling-disintegration-degradation	555:589	arg1	process					591:597	swelling-disintegration-degradation process	555:597	swelling-disintegration-degradation process of the alginate structure	555:623	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	0	22	theme	alginate-nanohydroxyapatite	31:57	arg1	hydrogel					59:66	alginate-nanohydroxyapatite hydrogel	31:66	alginate-nanohydroxyapatite hydrogel	31:66	Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections.					
31857183	5	23	theme	osteogenic	904:913	arg1	response					934:941	A strong osteogenic and mineralization response	895:941	A strong osteogenic and mineralization response	895:941	A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite.					
31857183	3	24	theme	structure	615:623	arg1	process					591:597	swelling-disintegration-degradation process	555:597	swelling-disintegration-degradation process of the alginate structure	555:623	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	2	25	theme	hydrogel	474:481	arg1	morphology					483:492	hydrogel morphology	474:492	hydrogel morphology	474:492	Bacteriophages were efficiently encapsulated, without jeopardizing phage viability and functionality, nor affecting hydrogel morphology and chemical composition.					
31857183	3	26	theme	Good	665:668	arg1	response					677:684	environmental pH. Good tissue response	647:684	environmental pH. Good tissue response	647:684	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	6	27	dep	showed	1065:1070	arg1	inhibiting					1105:1114	inhibiting	1105:1114	showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues	1065:1220	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	1	28	theme	multifunctional	265:279	arg1	system					171:176	An innovative delivery system	148:176	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel	148:244	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	1	28	theme	multifunctional	265:279	arg1	approach					281:288	a multifunctional approach	263:288	a multifunctional approach for local tissue regeneration and infection prevention and control	263:355	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	3	29	theme	environmental	647:659	arg1	response					677:684	environmental pH. Good tissue response	647:684	environmental pH. Good tissue response	647:684	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	5	30	theme	mineralization	919:932	arg1	response					934:941	A strong osteogenic and mineralization response	895:941	A strong osteogenic and mineralization response	895:941	A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite.					
31857183	4	31	theme	osteoblastic	845:856	arg1	morphology					883:892	morphology	883:892	morphology	883:892	Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology.					
31857183	4	31	theme	osteoblastic	845:856	arg1	proliferation					865:877	proliferation	865:877	proliferation	865:877	Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology.					
31857183	4	31	theme	osteoblastic	845:856	arg1	cells					858:862	osteoblastic cells	845:862	osteoblastic cells' proliferation and morphology	845:892	Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology.					
31857183	6	32	dep	attachment	1120:1129	arg1	the					1116:1118	the	1116:1118	the	1116:1118	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	7	33	theme	bone	1357:1360	arg1	integration					1362:1372	bone integration	1357:1372	bone integration	1357:1372	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	3	34	theme	biosafety	779:787	arg1	profile					789:795	their biosafety profile	773:795	their biosafety profile	773:795	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	3	35	theme	alginate	606:613	arg1	structure					615:623	the alginate structure	602:623	the alginate structure	602:623	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	0	36	theme	novel	73:77	arg1	system					88:93	a novel delivery system	71:93	a novel delivery system	71:93	Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections.					
31857183	3	37	theme	Bacteriophage	520:532	arg1	delivery					534:541	Bacteriophage delivery	520:541	Bacteriophage delivery	520:541	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	1	38	theme	innovative	151:160	arg1	system					171:176	An innovative delivery system	148:176	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel	148:244	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	1	38	theme	innovative	151:160	arg1	approach					281:288	a multifunctional approach	263:288	a multifunctional approach for local tissue regeneration and infection prevention and control	263:355	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	7	39	theme	bacterial	1309:1317	arg1	contamination					1319:1331	bacterial contamination	1309:1331	bacterial contamination	1309:1331	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	1	40	theme	delivery	162:169	arg1	system					171:176	An innovative delivery system	148:176	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel	148:244	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	1	40	theme	delivery	162:169	arg1	approach					281:288	a multifunctional approach	263:288	a multifunctional approach for local tissue regeneration and infection prevention and control	263:355	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	1	41	theme	local	294:298	arg1	regeneration					307:318	local tissue regeneration	294:318	local tissue regeneration	294:318	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	5	42	theme	strong	897:902	arg1	response					934:941	A strong osteogenic and mineralization response	895:941	A strong osteogenic and mineralization response	895:941	A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite.					
31857183	6	43	theme	antimicrobial	1082:1094	arg1	activity					1096:1103	excellent antimicrobial activity	1072:1103	excellent antimicrobial activity	1072:1103	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	5	44	with	system	994:999	arg1	nanohydroxyapatite					1006:1023	nanohydroxyapatite	1006:1023	nanohydroxyapatite	1006:1023	A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite.					
31857183	7	45	theme	promising	1267:1275	arg1	approach					1277:1284	a promising approach	1265:1284	a promising approach to prevent and control bacterial contamination during implantation and bone integration	1265:1372	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	7	45	theme	promising	1267:1275	arg1	approach					1247:1254	This new local delivery approach	1223:1254	This new local delivery approach	1223:1254	This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.					
31857183	2	46	theme	phage	425:429	arg1	viability					431:439	phage viability	425:439	phage viability	425:439	Bacteriophages were efficiently encapsulated, without jeopardizing phage viability and functionality, nor affecting hydrogel morphology and chemical composition.					
31857183	1	47	theme	tissue	300:305	arg1	regeneration					307:318	local tissue regeneration	294:318	local tissue regeneration	294:318	An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control.					
31857183	6	48	theme	excellent	1072:1080	arg1	activity					1096:1103	excellent antimicrobial activity	1072:1103	excellent antimicrobial activity	1072:1103	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	3	49	theme	tissue	670:675	arg1	response					677:684	environmental pH. Good tissue response	647:684	environmental pH. Good tissue response	647:684	Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile.					
31857183	0	50	theme	delivery	79:86	arg1	system					88:93	a novel delivery system	71:93	a novel delivery system	71:93	Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections.					
31857183	6	51	theme	femoral	1206:1212	arg1	tissues					1214:1220	femoral tissues	1206:1220	femoral tissues	1206:1220	Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues.					
31857183	4	52	dep	cells	858:862	arg1	morphology					883:892	morphology	883:892	morphology	883:892	Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology.					
31857183	4	52	dep	cells	858:862	arg1	proliferation					865:877	proliferation	865:877	proliferation	865:877	Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology.					
31857183	4	52	dep	cells	858:862	arg1	cells					858:862	osteoblastic cells	845:862	osteoblastic cells' proliferation and morphology	845:892	Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology.					
31857183	4	53	theme	Bacteriophages-loaded	798:818	arg1	hydrogels					820:828	Bacteriophages-loaded hydrogels	798:828	Bacteriophages-loaded hydrogels	798:828	Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology.					
30688188	5	0	dep	resulted	1082:1089	arg1	compared					1342:1349	compared	1342:1349	compared with NC	1342:1357	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	3	1	from	impact	608:613	arg1	composition					762:772	the human faecal microbiota composition	734:772	the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis	734:820	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	0	2	theme	gut	134:136	arg1	microbiota					138:147	the gut microbiota	130:147	the gut microbiota	130:147	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	5	3	theme	sd	1161:1162	arg1	h					1140:1140	24 h	1137:1140	24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1137:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	3	theme	sd	1161:1162	arg1	mm					1167:1168	86 (sd 27) v. 28 (sd 5) mm	1143:1168	86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05	1143:1180	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	8	4	theme	food	1776:1779	arg1	matrix					1781:1786	the complex food matrix	1764:1786	the complex food matrix	1764:1786	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	6	5	theme	Bacteriodetes	1468:1480	arg1	effects					1482:1488	the Bacteriodetes effects	1464:1488	the Bacteriodetes effects of oats	1464:1496	Considering the β-glucan treatment induced an increase in the phylum Bacteroidetes at 24 h, it explains the Bacteriodetes effects of oats as a food matrix.					
30688188	6	5	theme	Bacteriodetes	1468:1480	arg1	matrix					1508:1513	a food matrix	1501:1513	a food matrix	1501:1513	Considering the β-glucan treatment induced an increase in the phylum Bacteroidetes at 24 h, it explains the Bacteriodetes effects of oats as a food matrix.					
30688188	1	6	theme	prebiotic	222:230	arg1	properties					232:241	prebiotic properties	222:241	prebiotic properties	222:241	Wholegrain oats are known to modulate the human gut microbiota and have prebiotic properties (increase the growth of some health-promoting bacterial genera within the colon).					
30688188	5	7	dep	%	1065:1065	arg1	w/v					1068:1070	w/v	1068:1070	w/v	1068:1070	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	1	8	theme	Wholegrain	150:159	arg1	oats					161:164	Wholegrain oats	150:164	Wholegrain oats	150:164	Wholegrain oats are known to modulate the human gut microbiota and have prebiotic properties (increase the growth of some health-promoting bacterial genera within the colon).					
30688188	8	9	theme	main	1805:1808	arg1	β-glucan					1820:1827	its main bioactive β-glucan	1801:1827	its main bioactive β-glucan	1801:1827	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	5	10	theme	P	1315:1315	arg1	&lt					1317:1319	P &lt;0·05	1315:1324	P &lt;0·05	1315:1324	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	10	theme	P	1315:1315	arg1	adolescentis					1301:1312	Bifidobacterium adolescentis	1285:1312	Bifidobacterium adolescentis (P &lt;0·05)	1285:1325	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	3	11	theme	oat	659:661	arg1	concentrations					677:690	matched concentrations	669:690	matched concentrations of β-glucan extract or polyphenol mix	669:728	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	3	11	theme	oat	659:661	arg1	bran					663:666	oat bran	659:666	oat bran	659:666	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	0	12	theme	in	99:100	arg1	model					121:125	an in vitro fermentation model	96:125	an in vitro fermentation model of the gut microbiota	96:147	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	2	13	theme	gut	506:508	arg1	microbiota					510:519	gut microbiota	506:519	gut microbiota	506:519	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	0	14	theme	fermentation	108:119	arg1	model					121:125	an in vitro fermentation model	96:125	an in vitro fermentation model of the gut microbiota	96:147	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	1	15	contain	have	217:220	arg2	properties					232:241	prebiotic properties	222:241	prebiotic properties	222:241	Wholegrain oats are known to modulate the human gut microbiota and have prebiotic properties (increase the growth of some health-promoting bacterial genera within the colon).					
30688188	1	15	contain	have	217:220	arg1	oats					161:164	Wholegrain oats	150:164	Wholegrain oats	150:164	Wholegrain oats are known to modulate the human gut microbiota and have prebiotic properties (increase the growth of some health-promoting bacterial genera within the colon).					
30688188	3	16	theme	mix	726:728	arg1	concentrations					677:690	matched concentrations	669:690	matched concentrations of β-glucan extract or polyphenol mix	669:728	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	3	16	theme	mix	726:728	arg1	bran					663:666	oat bran	659:666	oat bran	659:666	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	6	17	from	increase	1406:1413	arg1	phylum					1422:1427	the phylum	1418:1427	the phylum	1418:1427	Considering the β-glucan treatment induced an increase in the phylum Bacteroidetes at 24 h, it explains the Bacteriodetes effects of oats as a food matrix.					
30688188	5	18	from	increase	1106:1113	arg1	h					1140:1140	24 h	1137:1140	24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1137:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	18	from	increase	1106:1113	arg1	mm					1167:1168	86 (sd 27) v. 28 (sd 5) mm	1143:1168	86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05	1143:1180	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	18	from	increase	1106:1113	arg1	production					1123:1132	SCFA production	1118:1132	SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1118:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	4	19	theme	acetic	970:975	arg1	acid					991:994	acetic and propionic acid	970:994	acetic and propionic acid	970:994	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	5	20	theme	oat	1073:1075	arg1	bran					1077:1080	the 1 % (w/v) oat bran	1059:1080	the 1 % (w/v) oat bran	1059:1080	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	1	21	theme	bacterial	289:297	arg1	genera					299:304	some health-promoting bacterial genera	267:304	some health-promoting bacterial genera within the colon	267:321	Wholegrain oats are known to modulate the human gut microbiota and have prebiotic properties (increase the growth of some health-promoting bacterial genera within the colon).					
30688188	3	22	theme	batch-culture	541:553	arg1	experiments					555:565	In vitro anaerobic batch-culture experiments	522:565	In vitro anaerobic batch-culture experiments	522:565	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	0	23	dep	in	99:100	arg1	vitro					102:106	vitro	102:106	vitro	102:106	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	3	24	theme	microbiota	751:760	arg1	composition					762:772	the human faecal microbiota composition	734:772	the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis	734:820	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	5	25	from	effect	1201:1206	arg1	h					1140:1140	24 h	1137:1140	24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1137:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	25	from	effect	1201:1206	arg1	mm					1167:1168	86 (sd 27) v. 28 (sd 5) mm	1143:1168	86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05	1143:1180	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	25	from	effect	1201:1206	arg1	production					1123:1132	SCFA production	1118:1132	SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1118:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	2	26	theme	rich	419:422	arg1	oat					405:407	oat	405:407	oat	405:407	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	2	26	theme	rich	419:422	arg1	source					432:437	a rich dietary source	417:437	a rich dietary source	417:437	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	4	27	theme	propionic	981:989	arg1	acid					991:994	acetic and propionic acid	970:994	acetic and propionic acid	970:994	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	3	28	theme	human	738:742	arg1	composition					762:772	the human faecal microbiota composition	734:772	the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis	734:820	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	5	29	theme	%	1065:1065	arg1	bran					1077:1080	the 1 % (w/v) oat bran	1059:1080	the 1 % (w/v) oat bran	1059:1080	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	3	30	theme	β-glucan	695:702	arg1	extract					704:710	β-glucan extract	695:710	β-glucan extract	695:710	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	4	31	dep	h	910:910	arg1	Bacteroidetes					913:925	10 h, Bacteroidetes	907:925	Bacteroidetes	913:925	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	3	32	theme	RNA	784:786	arg1	sequencing					793:802	16S RNA gene sequencing	780:802	16S RNA gene sequencing	780:802	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	7	33	from	increase	1546:1553	arg1	family					1577:1582	Enterobacteriaceae family	1558:1582	Enterobacteriaceae family	1558:1582	The polyphenol mix induced an increase in Enterobacteriaceae family at 24 h.					
30688188	2	34	theme	polyphenols	442:452	arg1	oat					405:407	oat	405:407	oat	405:407	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	2	34	theme	polyphenols	442:452	arg1	source					432:437	a rich dietary source	417:437	a rich dietary source	417:437	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	0	35	theme	bifidogenic	74:84	arg1	effect					86:91	a bifidogenic effect	72:91	a bifidogenic effect	72:91	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	8	36	theme	propionic	1684:1692	arg1	bifidobacteria					1652:1665	bifidobacteria	1652:1665	bifidobacteria	1652:1665	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	8	36	theme	propionic	1684:1692	arg1	acid					1694:1697	propionic acid	1684:1697	propionic acid	1684:1697	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	5	37	theme	&lt	1173:1175	arg1	h					1140:1140	24 h	1137:1140	24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1137:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	37	theme	&lt	1173:1175	arg1	mm					1167:1168	86 (sd 27) v. 28 (sd 5) mm	1143:1168	86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05	1143:1180	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	9	38	theme	whole	1861:1865	arg1	food					1867:1870	a whole food	1859:1870	a whole food	1859:1870	Thus, oats as a whole food led to the greatest impact on the microbiota.					
30688188	0	39	theme	Oat	0:2	arg1	bran					4:7	Oat bran	0:7	Oat bran	0:7	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	7	40	theme	Enterobacteriaceae	1558:1575	arg1	family					1577:1582	Enterobacteriaceae family	1558:1582	Enterobacteriaceae family	1558:1582	The polyphenol mix induced an increase in Enterobacteriaceae family at 24 h.					
30688188	9	41	from	impact	1892:1897	arg1	microbiota					1906:1915	the microbiota	1902:1915	the microbiota	1902:1915	Thus, oats as a whole food led to the greatest impact on the microbiota.					
30688188	3	42	theme	SCFA	808:811	arg1	analysis					813:820	SCFA analysis	808:820	SCFA analysis	808:820	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	5	43	theme	significant	1094:1104	arg1	increase					1106:1113	significant increase	1094:1113	significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1094:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	44	theme	bifidogenic	1189:1199	arg1	effect					1201:1206	a bifidogenic effect	1187:1206	a bifidogenic effect	1187:1206	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	0	45	theme	isolated	22:29	arg1	β-glucans					41:49	its isolated bioactive β-glucans	18:49	its isolated bioactive β-glucans	18:49	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	7	46	theme	polyphenol	1520:1529	arg1	mix					1531:1533	The polyphenol mix	1516:1533	The polyphenol mix	1516:1533	The polyphenol mix induced an increase in Enterobacteriaceae family at 24 h.					
30688188	4	47	theme	P	928:928	arg1	&lt					930:932	P &lt;0·05	928:937	P &lt;0·05	928:937	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	4	47	theme	P	928:928	arg1	h					910:910	10 h	907:910	10 h	907:910	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	1	48	theme	human	192:196	arg1	microbiota					202:211	the human gut microbiota	188:211	the human gut microbiota	188:211	Wholegrain oats are known to modulate the human gut microbiota and have prebiotic properties (increase the growth of some health-promoting bacterial genera within the colon).					
30688188	4	49	with	Supplementation	823:837	arg1	oats					844:847	oats	844:847	oats	844:847	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	8	50	theme	oat	1743:1745	arg1	compounds					1747:1755	all oat compounds	1739:1755	all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols	1739:1842	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	6	51	theme	oats	1493:1496	arg1	effects					1482:1488	the Bacteriodetes effects	1464:1488	the Bacteriodetes effects of oats	1464:1496	Considering the β-glucan treatment induced an increase in the phylum Bacteroidetes at 24 h, it explains the Bacteriodetes effects of oats as a food matrix.					
30688188	6	51	theme	oats	1493:1496	arg1	matrix					1508:1513	a food matrix	1501:1513	a food matrix	1501:1513	Considering the β-glucan treatment induced an increase in the phylum Bacteroidetes at 24 h, it explains the Bacteriodetes effects of oats as a food matrix.					
30688188	5	52	theme	Bifidobacterium	1285:1299	arg1	&lt					1317:1319	P &lt;0·05	1315:1324	P &lt;0·05	1315:1324	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	52	theme	Bifidobacterium	1285:1299	arg1	adolescentis					1301:1312	Bifidobacterium adolescentis	1285:1312	Bifidobacterium adolescentis (P &lt;0·05)	1285:1325	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	3	53	theme	doses	632:636	arg1	impact					608:613	the impact	604:613	the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis	604:820	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	0	54	theme	microbiota	138:147	arg1	model					121:125	an in vitro fermentation model	96:125	an in vitro fermentation model of the gut microbiota	96:147	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	5	55	theme	Bifidobacterium	1246:1260	arg1	&lt					1317:1319	P &lt;0·05	1315:1324	P &lt;0·05	1315:1324	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	55	theme	Bifidobacterium	1246:1260	arg1	abundance					1233:1241	the relative abundance	1220:1241	the relative abundance of Bifidobacterium unassigned at 10 h	1220:1279	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	55	theme	Bifidobacterium	1246:1260	arg1	adolescentis					1301:1312	Bifidobacterium adolescentis	1285:1312	Bifidobacterium adolescentis (P &lt;0·05)	1285:1325	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	2	56	theme	microbiota	510:519	arg1	modulation					492:501	the positive modulation	479:501	the positive modulation of gut microbiota	479:519	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	8	57	theme	complex	1768:1774	arg1	matrix					1781:1786	the complex food matrix	1764:1786	the complex food matrix	1764:1786	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	5	58	dep	effect	1201:1206	arg1	increasing					1209:1218	increasing	1209:1218	increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h	1209:1340	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	59	theme	v.	1154:1155	arg1	h					1140:1140	24 h	1137:1140	24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1137:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	59	theme	v.	1154:1155	arg1	mm					1167:1168	86 (sd 27) v. 28 (sd 5) mm	1143:1168	86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05	1143:1180	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	3	60	dep	In	522:523	arg1	vitro					525:529	vitro	525:529	vitro	525:529	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	0	61	contain	have	67:70	arg2	effect					86:91	a bifidogenic effect	72:91	a bifidogenic effect	72:91	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	0	61	contain	have	67:70	arg1	β-glucans					41:49	its isolated bioactive β-glucans	18:49	its isolated bioactive β-glucans	18:49	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	0	61	contain	have	67:70	arg1	bran					4:7	Oat bran	0:7	Oat bran	0:7	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	3	62	theme	In	522:523	arg1	experiments					555:565	In vitro anaerobic batch-culture experiments	522:565	In vitro anaerobic batch-culture experiments	522:565	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	8	63	theme	bioactive	1810:1818	arg1	β-glucan					1820:1827	its main bioactive β-glucan	1801:1827	its main bioactive β-glucan	1801:1827	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	3	64	theme	bran	663:666	arg1	doses					632:636	two different doses	618:636	two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix,	618:729	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	2	65	theme	positive	483:490	arg1	modulation					492:501	the positive modulation	479:501	the positive modulation of gut microbiota	479:519	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	3	66	theme	anaerobic	531:539	arg1	experiments					555:565	In vitro anaerobic batch-culture experiments	522:565	In vitro anaerobic batch-culture experiments	522:565	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	2	67	theme	dietary	424:430	arg1	oat					405:407	oat	405:407	oat	405:407	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	2	67	theme	dietary	424:430	arg1	source					432:437	a rich dietary source	417:437	a rich dietary source	417:437	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	4	68	theme	acid	991:994	arg1	concentrations					952:965	concentrations	952:965	concentrations of acetic and propionic acid	952:994	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	3	69	theme	matched	669:675	arg1	concentrations					677:690	matched concentrations	669:690	matched concentrations of β-glucan extract or polyphenol mix	669:728	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	3	69	theme	matched	669:675	arg1	bran					663:666	oat bran	659:666	oat bran	659:666	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	1	70	theme	genera	299:304	arg1	growth					257:262	the growth	253:262	the growth of some health-promoting bacterial genera within the colon	253:321	Wholegrain oats are known to modulate the human gut microbiota and have prebiotic properties (increase the growth of some health-promoting bacterial genera within the colon).					
30688188	5	71	theme	1	1063:1063	arg1	%					1065:1065	%	1065:1065	%	1065:1065	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	3	72	theme	polyphenol	715:724	arg1	mix					726:728	polyphenol mix	715:728	polyphenol mix	715:728	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	3	73	theme	faecal	744:749	arg1	composition					762:772	the human faecal microbiota composition	734:772	the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis	734:820	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	3	74	theme	extract	704:710	arg1	concentrations					677:690	matched concentrations	669:690	matched concentrations of β-glucan extract or polyphenol mix	669:728	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	3	74	theme	extract	704:710	arg1	bran					663:666	oat bran	659:666	oat bran	659:666	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	3	75	theme	gene	788:791	arg1	sequencing					793:802	16S RNA gene sequencing	780:802	16S RNA gene sequencing	780:802	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	5	76	from	h	1140:1140	arg1	effect					1201:1206	a bifidogenic effect	1187:1206	a bifidogenic effect	1187:1206	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	76	from	h	1140:1140	arg1	increase					1106:1113	significant increase	1094:1113	significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1094:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	76	from	h	1140:1140	arg1	production					1123:1132	SCFA production	1118:1132	SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1118:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	6	77	theme	β-glucan	1376:1383	arg1	treatment					1385:1393	the β-glucan treatment	1372:1393	the β-glucan treatment	1372:1393	Considering the β-glucan treatment induced an increase in the phylum Bacteroidetes at 24 h, it explains the Bacteriodetes effects of oats as a food matrix.					
30688188	5	78	theme	P	1171:1171	arg1	&lt					1173:1175	P &lt	1171:1175	86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05	1143:1180	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	79	theme	SCFA	1118:1121	arg1	production					1123:1132	SCFA production	1118:1132	SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1118:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	3	80	theme	16S	780:782	arg1	sequencing					793:802	16S RNA gene sequencing	780:802	16S RNA gene sequencing	780:802	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	5	81	theme	bran	1077:1080	arg1	Fermentation					1043:1054	Fermentation	1043:1054	Fermentation of the 1 % (w/v) oat bran	1043:1080	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	2	82	theme	fibre	381:385	arg1	content					387:393	the fibre content	377:393	the fibre content	377:393	Research to date mainly attributes these effects to the fibre content; however, oat is also a rich dietary source of polyphenols, which may contribute to the positive modulation of gut microbiota.					
30688188	5	83	theme	relative	1224:1231	arg1	abundance					1233:1241	the relative abundance	1220:1241	the relative abundance of Bifidobacterium unassigned at 10 h	1220:1279	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	8	84	theme	acetic	1668:1673	arg1	bifidobacteria					1652:1665	bifidobacteria	1652:1665	bifidobacteria	1652:1665	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	8	84	theme	acetic	1668:1673	arg1	acid					1675:1678	acetic acid	1668:1678	acetic acid	1668:1678	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	3	85	dep	doses	632:636	arg1	w/v					650:652	w/v	650:652	w/v	650:652	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	3	85	dep	doses	632:636	arg1	%					647:647	1 and 3 %	639:647	1 and 3 % (w/v)	639:653	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	4	86	theme	Proteobacteria	876:889	arg1	abundance					863:871	the abundance	859:871	the abundance of Proteobacteria (P &lt;0·01)	859:902	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	9	87	theme	greatest	1883:1890	arg1	impact					1892:1897	the greatest impact	1879:1897	the greatest impact on the microbiota	1879:1915	Thus, oats as a whole food led to the greatest impact on the microbiota.					
30688188	0	88	theme	bioactive	31:39	arg1	β-glucans					41:49	its isolated bioactive β-glucans	18:49	its isolated bioactive β-glucans	18:49	Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota.					
30688188	4	89	theme	P	892:892	arg1	Proteobacteria					876:889	Proteobacteria	876:889	Proteobacteria (P &lt;0·01)	876:902	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	4	89	theme	P	892:892	arg1	&lt					894:896	P &lt;0·01	892:901	P &lt;0·01	892:901	Supplementation with oats increased the abundance of Proteobacteria (P &lt;0·01) at 10 h, Bacteroidetes (P &lt;0·05) at 24 h and concentrations of acetic and propionic acid increased at 10 and 24 h compared with the NC.					
30688188	5	90	theme	sd	1147:1148	arg1	h					1140:1140	24 h	1137:1140	24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05)	1137:1181	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	5	90	theme	sd	1147:1148	arg1	mm					1167:1168	86 (sd 27) v. 28 (sd 5) mm	1143:1168	86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05	1143:1180	Fermentation of the 1 % (w/v) oat bran resulted in significant increase in SCFA production at 24 h (86 (sd 27) v. 28 (sd 5) mm; P &lt;0·05) and a bifidogenic effect, increasing the relative abundance of Bifidobacterium unassigned at 10 h and Bifidobacterium adolescentis (P &lt;0·05) at 10 and 24 h compared with NC.					
30688188	1	91	theme	health-promoting	272:287	arg1	genera					299:304	some health-promoting bacterial genera	267:304	some health-promoting bacterial genera within the colon	267:321	Wholegrain oats are known to modulate the human gut microbiota and have prebiotic properties (increase the growth of some health-promoting bacterial genera within the colon).					
30688188	6	92	theme	food	1503:1506	arg1	effects					1482:1488	the Bacteriodetes effects	1464:1488	the Bacteriodetes effects of oats	1464:1496	Considering the β-glucan treatment induced an increase in the phylum Bacteroidetes at 24 h, it explains the Bacteriodetes effects of oats as a food matrix.					
30688188	6	92	theme	food	1503:1506	arg1	matrix					1508:1513	a food matrix	1501:1513	a food matrix	1501:1513	Considering the β-glucan treatment induced an increase in the phylum Bacteroidetes at 24 h, it explains the Bacteriodetes effects of oats as a food matrix.					
30688188	8	93	theme	compounds	1747:1755	arg1	synergy					1728:1734	the synergy	1724:1734	the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols	1724:1842	In conclusion, in this study, we found that oats increased bifidobacteria, acetic acid and propionic acid, and this is mediated by the synergy of all oat compounds within the complex food matrix, rather than its main bioactive β-glucan or polyphenols.					
30688188	3	94	theme	different	622:630	arg1	doses					632:636	two different doses	618:636	two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix,	618:729	In vitro anaerobic batch-culture experiments were performed over 24 h to evaluate the impact of two different doses (1 and 3 % (w/v)) of oat bran, matched concentrations of β-glucan extract or polyphenol mix, on the human faecal microbiota composition using 16S RNA gene sequencing and SCFA analysis.					
30688188	1	95	theme	gut	198:200	arg1	microbiota					202:211	the human gut microbiota	188:211	the human gut microbiota	188:211	Wholegrain oats are known to modulate the human gut microbiota and have prebiotic properties (increase the growth of some health-promoting bacterial genera within the colon).					
30496992	9	0	theme	low	1612:1614	arg1	toxicity					1616:1623	low toxicity	1612:1623	low toxicity to test organisms	1612:1641	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	6	1	theme	use	1090:1092	arg1	aspect					1060:1065	the aspect	1056:1065	the aspect of their environmental use	1056:1092	Phytotoxkit and Microtox tests were performed in order to determine the ecotoxicity of the wastes in the aspect of their environmental use.					
30496992	10	2	theme	> cellulose	1772:1782	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	1	3	attach	present	140:146	arg1	production					161:170	cellulose production waste and municipal sewage sludge condition	151:214	production	161:170	Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment.					
30496992	1	3	attach	present	140:146	arg1	condition					206:214	municipal sewage sludge condition	182:214	municipal sewage sludge condition	182:214	Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment.					
30496992	1	3	attach	present	140:146	arg2	contaminants					127:138	Chemical contaminants	118:138	Chemical contaminants	118:138	Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment.					
30496992	3	4	theme	cellulose	528:536	arg1	production					538:547	cellulose production waste and municipal sewage sludge	528:581	production	538:547	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	12	5	theme	municipal	2019:2027	arg1	sludge					2036:2041	municipal sewage sludge	2019:2041	municipal sewage sludge	2019:2041	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	5	6	theme	wastes	777:782	arg1	composition					758:768	the chemical composition	745:768	the chemical composition of the wastes	745:782	The research on the chemical composition of the wastes was conducted under laboratory conditions, determining the content of macroelements, microelements and heavy metals with respect to using these wastes in the environment.					
30496992	10	7	dep	phytotoxicity	1707:1719	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	10	8	theme	cellulose	1745:1753	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	1	9	theme	municipal	182:190	arg1	condition					206:214	municipal sewage sludge condition	182:214	municipal sewage sludge condition	182:214	Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment.					
30496992	12	10	theme	cellulose	1988:1996	arg1	production					1998:2007	cellulose production waste and municipal sewage sludge	1988:2041	production	1998:2007	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	6	11	theme	Microtox	971:978	arg1	tests					980:984	Phytotoxkit and Microtox tests	955:984	Phytotoxkit and Microtox tests	955:984	Phytotoxkit and Microtox tests were performed in order to determine the ecotoxicity of the wastes in the aspect of their environmental use.					
30496992	3	12	theme	sludge	576:581	arg1	ecotoxicity					513:523	ecotoxicity	513:523	ecotoxicity	513:523	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	3	12	theme	sludge	576:581	arg1	composition					497:507	chemical composition	488:507	chemical composition	488:507	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	5	13	from	research	733:740	arg1	composition					758:768	the chemical composition	745:768	the chemical composition of the wastes	745:782	The research on the chemical composition of the wastes was conducted under laboratory conditions, determining the content of macroelements, microelements and heavy metals with respect to using these wastes in the environment.					
30496992	1	14	theme	sludge	199:204	arg1	condition					206:214	municipal sewage sludge condition	182:214	municipal sewage sludge condition	182:214	Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment.					
30496992	6	15	theme	Phytotoxkit	955:965	arg1	tests					980:984	Phytotoxkit and Microtox tests	955:984	Phytotoxkit and Microtox tests	955:984	Phytotoxkit and Microtox tests were performed in order to determine the ecotoxicity of the wastes in the aspect of their environmental use.					
30496992	10	16	theme	production	1784:1793	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	12	17	theme	variable	1955:1962	arg1	composition					1973:1983	the variable chemical composition	1951:1983	the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity	1951:2070	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	10	18	theme	waste	1766:1770	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	3	19	theme	eco-friendly	601:612	arg1	application					614:624	their eco-friendly application	595:624	their eco-friendly application	595:624	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	4	20	theme	established	664:674	arg1	environment					681:691	the established soil environment	660:691	the established soil environment according to regulations in Poland	660:726	The assessment was done based on the established soil environment according to regulations in Poland.					
30496992	10	21	theme	production	1820:1829	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	9	22	theme	cellulose	1483:1491	arg1	waste					1504:1508	the cellulose production waste	1479:1508	the cellulose production waste	1479:1508	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	9	22	theme	cellulose	1483:1491	arg1	toxic					1514:1518	toxic	1514:1518	toxic	1514:1518	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	4	23	theme	soil	676:679	arg1	environment					681:691	the established soil environment	660:691	the established soil environment according to regulations in Poland	660:726	The assessment was done based on the established soil environment according to regulations in Poland.					
30496992	10	24	from	phytotoxicity	1707:1719	arg1	order					1738:1742	the following order	1724:1742	the following order	1724:1742	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	5	25	theme	chemical	749:756	arg1	composition					758:768	the chemical composition	745:768	the chemical composition of the wastes	745:782	The research on the chemical composition of the wastes was conducted under laboratory conditions, determining the content of macroelements, microelements and heavy metals with respect to using these wastes in the environment.					
30496992	10	26	theme	+ soil	1801:1806	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	5	27	theme	metals	893:898	arg1	content					843:849	the content	839:849	the content of macroelements, microelements and heavy metals	839:898	The research on the chemical composition of the wastes was conducted under laboratory conditions, determining the content of macroelements, microelements and heavy metals with respect to using these wastes in the environment.					
30496992	0	28	theme	new	65:67	arg1	material					69:76	new material	65:76	new material for an ecological management of wastes	65:115	A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.					
30496992	1	29	theme	cellulose	151:159	arg1	production					161:170	cellulose production waste and municipal sewage sludge condition	151:214	production	161:170	Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment.					
30496992	3	30	theme	application	614:624	arg1	terms					586:590	terms	586:590	terms of their eco-friendly application	586:624	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	6	31	from	ecotoxicity	1027:1037	arg1	aspect					1060:1065	the aspect	1056:1065	the aspect of their environmental use	1056:1092	Phytotoxkit and Microtox tests were performed in order to determine the ecotoxicity of the wastes in the aspect of their environmental use.					
30496992	1	32	theme	Chemical	118:125	arg1	contaminants					127:138	Chemical contaminants	118:138	Chemical contaminants	118:138	Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment.					
30496992	7	33	theme	sewage	1178:1183	arg1	sludge					1185:1190	the municipal sewage sludge	1164:1190	the municipal sewage sludge used in the incubation experiment	1164:1224	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	2	34	theme	fertilising	434:444	arg1	value					446:450	the considerable fertilising value	417:450	organic substances as well as the considerable fertilising value	387:450	Environmental use of the these wastes appears to be justified owing to the content of organic substances as well as the considerable fertilising value.					
30496992	12	35	dep	composition	1973:1983	arg1	respect					1940:1946	respect	1940:1946	respect	1940:1946	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	8	36	theme	permissible	1343:1353	arg1	content					1355:1361	the permissible content	1339:1361	the permissible content that conditions their use in agriculture and reclamation	1339:1418	The content of heavy metals in the waste did not exceed the permissible content that conditions their use in agriculture and reclamation.					
30496992	11	37	theme	waste	1889:1893	arg1	mixtures					1895:1902	waste mixtures	1889:1902	waste mixtures	1889:1902	The research results indicate that waste mixtures qualify for environmental use.					
30496992	5	38	theme	laboratory	804:813	arg1	conditions					815:824	laboratory conditions	804:824	laboratory conditions	804:824	The research on the chemical composition of the wastes was conducted under laboratory conditions, determining the content of macroelements, microelements and heavy metals with respect to using these wastes in the environment.					
30496992	9	39	theme	cellulose	1548:1556	arg1	production					1558:1567	cellulose production waste and sewage sludge	1548:1591	production	1558:1567	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	10	40	theme	applied	1648:1654	arg1	wastes					1656:1661	The applied wastes	1644:1661	The applied wastes	1644:1661	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	6	41	theme	wastes	1046:1051	arg1	ecotoxicity					1027:1037	the ecotoxicity	1023:1037	the ecotoxicity of the wastes in the aspect of their environmental use	1023:1092	Phytotoxkit and Microtox tests were performed in order to determine the ecotoxicity of the wastes in the aspect of their environmental use.					
30496992	11	42	theme	environmental	1916:1928	arg1	use					1930:1932	environmental use	1916:1932	environmental use	1916:1932	The research results indicate that waste mixtures qualify for environmental use.					
30496992	12	43	theme	ecotoxicological	2182:2197	arg1	tests					2199:2203	ecotoxicological tests	2182:2203	ecotoxicological tests	2182:2203	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	2	44	theme	substances	395:404	arg1	content					376:382	the content	372:382	the content of organic substances as well as the considerable fertilising value	372:450	Environmental use of the these wastes appears to be justified owing to the content of organic substances as well as the considerable fertilising value.					
30496992	7	45	theme	cellulose	1136:1144	arg1	waste					1157:1161	the cellulose production waste	1132:1161	the cellulose production waste	1132:1161	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	0	46	theme	production	23:32	arg1	waste					34:38	cellulose production waste	13:38	cellulose production waste	13:38	A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.					
30496992	9	47	theme	sewage	1579:1584	arg1	sludge					1586:1591	sewage sludge	1579:1591	sewage sludge	1579:1591	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	5	48	theme	heavy	887:891	arg1	metals					893:898	heavy metals	887:898	heavy metals	887:898	The research on the chemical composition of the wastes was conducted under laboratory conditions, determining the content of macroelements, microelements and heavy metals with respect to using these wastes in the environment.					
30496992	10	49	theme	+ sewage	1837:1844	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	1	50	dep	production	161:170	arg1	waste					172:176	waste	172:176	waste	172:176	Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment.					
30496992	3	51	theme	study	468:472	arg1	aim					457:459	The aim	453:459	The aim of the study	453:472	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	7	52	theme	microelements	1268:1280	arg1	content					1239:1245	a higher content	1230:1245	a higher content of macroelements and microelements	1230:1280	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	8	53	theme	metals	1304:1309	arg1	content					1287:1293	The content	1283:1293	The content of heavy metals in the waste	1283:1322	The content of heavy metals in the waste did not exceed the permissible content that conditions their use in agriculture and reclamation.					
30496992	7	54	theme	macroelements	1250:1262	arg1	content					1239:1245	a higher content	1230:1245	a higher content of macroelements and microelements	1230:1280	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	2	55	theme	organic	387:393	arg1	substances					395:404	organic substances	387:404	organic substances as well as the considerable fertilising value	387:450	Environmental use of the these wastes appears to be justified owing to the content of organic substances as well as the considerable fertilising value.					
30496992	3	56	dep	production	538:547	arg1	waste					549:553	waste	549:553	waste	549:553	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	3	57	theme	chemical	488:495	arg1	composition					497:507	chemical composition	488:507	chemical composition	488:507	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	12	58	theme	sewage	2029:2034	arg1	sludge					2036:2041	municipal sewage sludge	2019:2041	municipal sewage sludge	2019:2041	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	11	59	theme	research	1858:1865	arg1	results					1867:1873	The research results	1854:1873	The research results	1854:1873	The research results indicate that waste mixtures qualify for environmental use.					
30496992	6	60	theme	environmental	1076:1088	arg1	use					1090:1092	their environmental use	1070:1092	their environmental use	1070:1092	Phytotoxkit and Microtox tests were performed in order to determine the ecotoxicity of the wastes in the aspect of their environmental use.					
30496992	10	61	theme	following	1728:1736	arg1	order					1738:1742	the following order	1724:1742	the following order	1724:1742	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	12	62	theme	production	1998:2007	arg1	composition					1973:1983	the variable chemical composition	1951:1983	the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity	1951:2070	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	9	63	theme	test	1628:1631	arg1	organisms					1633:1641	test organisms	1628:1641	test organisms	1628:1641	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	8	64	from	use	1385:1387	arg1	reclamation					1408:1418	reclamation	1408:1418	reclamation	1408:1418	The content of heavy metals in the waste did not exceed the permissible content that conditions their use in agriculture and reclamation.					
30496992	8	64	from	use	1385:1387	arg1	agriculture					1392:1402	agriculture	1392:1402	agriculture	1392:1402	The content of heavy metals in the waste did not exceed the permissible content that conditions their use in agriculture and reclamation.					
30496992	5	65	theme	macroelements	854:866	arg1	content					843:849	the content	839:849	the content of macroelements, microelements and heavy metals	839:898	The research on the chemical composition of the wastes was conducted under laboratory conditions, determining the content of macroelements, microelements and heavy metals with respect to using these wastes in the environment.					
30496992	12	66	theme	environmental	2121:2133	arg1	use					2135:2137	environmental use	2121:2137	environmental use	2121:2137	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	10	67	theme	production	1755:1764	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	5	68	theme	microelements	869:881	arg1	content					843:849	the content	839:849	the content of macroelements, microelements and heavy metals	839:898	The research on the chemical composition of the wastes was conducted under laboratory conditions, determining the content of macroelements, microelements and heavy metals with respect to using these wastes in the environment.					
30496992	12	69	theme	chemical	1964:1971	arg1	composition					1973:1983	the variable chemical composition	1951:1983	the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity	1951:2070	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	0	70	theme	wastes	110:115	arg1	management					96:105	an ecological management	82:105	an ecological management of wastes	82:115	A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.					
30496992	2	71	theme	Environmental	301:313	arg1	use					315:317	Environmental use	301:317	Environmental use of the these wastes	301:337	Environmental use of the these wastes appears to be justified owing to the content of organic substances as well as the considerable fertilising value.					
30496992	1	72	theme	sewage	192:197	arg1	condition					206:214	municipal sewage sludge condition	182:214	municipal sewage sludge condition	182:214	Chemical contaminants present in cellulose production waste and municipal sewage sludge condition the necessity to treat these wastes before they are introduced into the environment.					
30496992	3	73	theme	municipal	559:567	arg1	sludge					576:581	municipal sewage sludge	559:581	municipal sewage sludge	559:581	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	10	74	theme	waste	1795:1799	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	3	75	theme	sewage	569:574	arg1	sludge					576:581	municipal sewage sludge	559:581	municipal sewage sludge	559:581	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	7	76	theme	production	1146:1155	arg1	waste					1157:1161	the cellulose production waste	1132:1161	the cellulose production waste	1132:1161	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	10	77	theme	waste	1831:1835	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	3	78	theme	production	538:547	arg1	ecotoxicity					513:523	ecotoxicity	513:523	ecotoxicity	513:523	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	3	78	theme	production	538:547	arg1	composition					497:507	chemical composition	488:507	chemical composition	488:507	The aim of the study was to assess chemical composition and ecotoxicity of cellulose production waste and municipal sewage sludge in terms of their eco-friendly application.					
30496992	7	79	theme	incubation	1204:1213	arg1	experiment					1215:1224	the incubation experiment	1200:1224	the incubation experiment	1200:1224	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	10	80	theme	> cellulose	1808:1818	arg1	sludge					1846:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge	1745:1851	The applied wastes and their mixtures were ranked according to phytotoxicity in the following order: cellulose production waste > cellulose production waste + soil > cellulose production waste + sewage sludge.					
30496992	9	81	theme	production	1493:1502	arg1	waste					1504:1508	the cellulose production waste	1479:1508	the cellulose production waste	1479:1508	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	9	81	theme	production	1493:1502	arg1	toxic					1514:1518	toxic	1514:1518	toxic	1514:1518	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	8	82	theme	heavy	1298:1302	arg1	metals					1304:1309	heavy metals	1298:1309	heavy metals	1298:1309	The content of heavy metals in the waste did not exceed the permissible content that conditions their use in agriculture and reclamation.					
30496992	7	83	contain	had	1226:1228	arg1	sludge					1185:1190	the municipal sewage sludge	1164:1190	the municipal sewage sludge used in the incubation experiment	1164:1224	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	7	83	contain	had	1226:1228	arg2	content					1239:1245	a higher content	1230:1245	a higher content of macroelements and microelements	1230:1280	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	12	84	theme	ecotoxicity	2060:2070	arg1	composition					1973:1983	the variable chemical composition	1951:1983	the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity	1951:2070	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	8	85	from	content	1287:1293	arg1	waste					1318:1322	the waste	1314:1322	the waste	1314:1322	The content of heavy metals in the waste did not exceed the permissible content that conditions their use in agriculture and reclamation.					
30496992	7	86	theme	municipal	1168:1176	arg1	sludge					1185:1190	the municipal sewage sludge	1164:1190	the municipal sewage sludge used in the incubation experiment	1164:1224	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	9	87	theme	bioassays	1445:1453	arg1	results					1434:1440	the results	1430:1440	the results of bioassays	1430:1453	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	2	88	theme	considerable	421:432	arg1	value					446:450	the considerable fertilising value	417:450	organic substances as well as the considerable fertilising value	387:450	Environmental use of the these wastes appears to be justified owing to the content of organic substances as well as the considerable fertilising value.					
30496992	0	89	theme	cellulose	13:21	arg1	waste					34:38	cellulose production waste	13:38	cellulose production waste	13:38	A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.					
30496992	9	90	theme	production	1558:1567	arg1	combination					1533:1543	the combination	1529:1543	the combination of cellulose production waste and sewage sludge	1529:1591	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	0	91	theme	waste	34:38	arg1	mixture					2:8	A mixture	0:8	A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.	0:116	A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.					
30496992	12	92	dep	production	1998:2007	arg1	waste					2009:2013	waste	2009:2013	waste	2009:2013	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	0	93	theme	ecological	85:94	arg1	management					96:105	an ecological management	82:105	an ecological management of wastes	82:115	A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.					
30496992	0	94	theme	municipal	45:53	arg1	sewage					55:60	municipal sewage	45:60	municipal sewage as new material for an ecological management of wastes	45:115	A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.					
30496992	9	95	theme	sludge	1586:1591	arg1	combination					1533:1543	the combination	1529:1543	the combination of cellulose production waste and sewage sludge	1529:1591	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	2	96	theme	value	446:450	arg1	content					376:382	the content	372:382	the content of organic substances as well as the considerable fertilising value	372:450	Environmental use of the these wastes appears to be justified owing to the content of organic substances as well as the considerable fertilising value.					
30496992	9	97	dep	production	1558:1567	arg1	waste					1569:1573	waste	1569:1573	waste	1569:1573	Based on the results of bioassays it was established that the cellulose production waste was toxic, whereas the combination of cellulose production waste and sewage sludge was found to be of low toxicity to test organisms.					
30496992	0	98	with	mixture	2:8	arg1	sewage					55:60	municipal sewage	45:60	municipal sewage as new material for an ecological management of wastes	45:115	A mixture of cellulose production waste with municipal sewage as new material for an ecological management of wastes.					
30496992	2	99	theme	wastes	332:337	arg1	use					315:317	Environmental use	301:317	Environmental use of the these wastes	301:337	Environmental use of the these wastes appears to be justified owing to the content of organic substances as well as the considerable fertilising value.					
30496992	4	100	from	regulations	706:716	arg1	Poland					721:726	Poland	721:726	Poland	721:726	The assessment was done based on the established soil environment according to regulations in Poland.					
30496992	12	101	theme	sludge	2036:2041	arg1	composition					1973:1983	the variable chemical composition	1951:1983	the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity	1951:2070	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
30496992	7	102	theme	higher	1232:1237	arg1	content					1239:1245	a higher content	1230:1245	a higher content of macroelements and microelements	1230:1280	It was established that, compared to the cellulose production waste, the municipal sewage sludge used in the incubation experiment had a higher content of macroelements and microelements.					
30496992	12	103	theme	chemical	2160:2167	arg1	analysis					2169:2176	chemical analysis	2160:2176	chemical analysis	2160:2176	With respect to the variable chemical composition of cellulose production waste and municipal sewage sludge as well as their ecotoxicity, it was established that each batch intended for environmental use must be subjected to chemical analysis and ecotoxicological tests.					
31059977	9	0	theme	immune	1576:1581	arg1	activities					1583:1592	its antioxidant, anti-inflammatory and enhancing immune activities	1527:1592	its antioxidant, anti-inflammatory and enhancing immune activities	1527:1592	In the meanwhile, EP-1 also showed its antioxidant, anti-inflammatory and enhancing immune activities.					
31059977	6	1	theme	intestinal	1058:1067	arg1	structure					1075:1083	the intestinal flora structure	1054:1083	the intestinal flora structure	1054:1083	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	0	2	theme	fatty	178:182	arg1	acids					184:188	short chain fatty acids	166:188	short chain fatty acids levels	166:195	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	3	3	theme	traditional	496:506	arg1	medicine					513:520	traditional folk medicine	496:520	traditional folk medicine	496:520	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	2	4	with	patients	381:388	arg1	disease					409:415	long-standing disease	395:415	long-standing disease	395:415	Risk of colorectal cancer in ulcerative colitis is increased in patients with long-standing disease compared with the general population.					
31059977	2	5	from	Risk	317:320	arg1	colitis					357:363	ulcerative colitis	346:363	ulcerative colitis	346:363	Risk of colorectal cancer in ulcerative colitis is increased in patients with long-standing disease compared with the general population.					
31059977	3	6	with	medicine	513:520	arg1	activities					618:627	anti-gastritis and anti-ulcerative colitis activities	575:627	anti-gastritis and anti-ulcerative colitis activities	575:627	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	10	7	theme	anti-UC	1658:1664	arg1	activity					1666:1673	anti-UC activity	1658:1673	anti-UC activity	1658:1673	Thus, the polysaccharide purified from HE showed potential for anti-UC activity and the complementary and alternative medicine (CAM) herb therapy.					
31059977	0	8	theme	GPR41/43	201:208	arg1	respectors					210:219	the gut microbiota, short chain fatty acids levels and GPR41/43 respectors	146:219	the gut microbiota, short chain fatty acids levels and GPR41/43 respectors	146:219	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	3	9	theme	medicinal	526:534	arg1	cuisine					536:542	medicinal cuisine	526:542	medicinal cuisine	526:542	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	10	10	theme	CAM	1723:1725	arg1	therapy					1733:1739	the complementary and alternative medicine (CAM) herb therapy	1679:1739	therapy	1733:1739	Thus, the polysaccharide purified from HE showed potential for anti-UC activity and the complementary and alternative medicine (CAM) herb therapy.					
31059977	10	11	theme	medicine	1713:1720	arg1	therapy					1733:1739	the complementary and alternative medicine (CAM) herb therapy	1679:1739	therapy	1733:1739	Thus, the polysaccharide purified from HE showed potential for anti-UC activity and the complementary and alternative medicine (CAM) herb therapy.					
31059977	7	12	theme	gut	1269:1271	arg1	community					1284:1292	the gut microbiota community	1265:1292	the gut microbiota community	1265:1292	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	4	13	theme	purified	638:645	arg1	polysaccharide					654:667	a purified unique polysaccharide	636:667	a purified unique polysaccharide isolated from HE mycelium	636:693	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	4	13	theme	purified	638:645	arg1	EP-1					630:633	EP-1	630:633	EP-1	630:633	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	1	14	theme	Ulcerative	222:231	arg1	UC					242:243	UC	242:243	UC	242:243	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	1	14	theme	Ulcerative	222:231	arg1	disorder					285:292	an idiopathic, chronic inflammatory disorder	249:292	disorder	285:292	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	1	14	theme	Ulcerative	222:231	arg1	colitis					233:239	Ulcerative colitis	222:239	Ulcerative colitis (UC)	222:244	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	7	15	theme	fatty	1320:1324	arg1	acids					1326:1330	short chain fatty acids	1308:1330	short chain fatty acids (SCFAs)	1308:1338	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	7	15	theme	fatty	1320:1324	arg1	SCFAs					1333:1337	SCFAs	1333:1337	SCFAs	1333:1337	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	0	16	theme	microbiota	154:163	arg1	respectors					210:219	the gut microbiota, short chain fatty acids levels and GPR41/43 respectors	146:219	the gut microbiota, short chain fatty acids levels and GPR41/43 respectors	146:219	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	10	17	theme	herb	1728:1731	arg1	therapy					1733:1739	the complementary and alternative medicine (CAM) herb therapy	1679:1739	therapy	1733:1739	Thus, the polysaccharide purified from HE showed potential for anti-UC activity and the complementary and alternative medicine (CAM) herb therapy.					
31059977	7	18	theme	short	1308:1312	arg1	acids					1326:1330	short chain fatty acids	1308:1330	short chain fatty acids (SCFAs)	1308:1338	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	7	18	theme	short	1308:1312	arg1	SCFAs					1333:1337	SCFAs	1333:1337	SCFAs	1333:1337	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	0	19	theme	short	166:170	arg1	acids					184:188	short chain fatty acids	166:188	short chain fatty acids levels	166:195	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	6	20	theme	16S	991:993	arg1	sequencing					1000:1009	16S rRNA sequencing	991:1009	16S rRNA sequencing of the rat colonic contents	991:1037	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	6	21	theme	certain	1159:1165	arg1	degree					1167:1172	a certain degree	1157:1172	a certain degree	1157:1172	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	7	22	theme	further	1187:1193	arg1	results					1195:1201	The further results	1183:1201	The further results	1183:1201	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	3	23	theme	colitis	610:616	arg1	activities					618:627	anti-gastritis and anti-ulcerative colitis activities	575:627	anti-gastritis and anti-ulcerative colitis activities	575:627	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	0	24	theme	ulcerative	89:98	arg1	colitis					100:106	acetic acid-induced ulcerative colitis	69:106	acetic acid-induced ulcerative colitis rats	69:111	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	0	25	theme	levels	190:195	arg1	respectors					210:219	the gut microbiota, short chain fatty acids levels and GPR41/43 respectors	146:219	the gut microbiota, short chain fatty acids levels and GPR41/43 respectors	146:219	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	6	26	theme	Illumina	966:973	arg1	platform					981:988	the Illumina MiSeq platform	962:988	the Illumina MiSeq platform	962:988	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	4	27	theme	anti-	765:769	arg1	colitis					782:788	anti- ulcerative colitis	765:788	anti- ulcerative colitis activity	765:797	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	5	28	theme	rats	947:950	arg1	symptoms					912:919	the symptoms	908:919	the symptoms of acetic acid induced UC rats	908:950	In this study, our data shows that EP-1 was effective in relieving the symptoms of acetic acid induced UC rats.					
31059977	0	29	from	mycelium	48:55	arg1	polysaccharide					9:22	A unique polysaccharide	0:22	A unique polysaccharide from Hericium erinaceus mycelium	0:55	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	4	30	theme	colitis	782:788	arg1	activity					790:797	anti- ulcerative colitis activity	765:797	anti- ulcerative colitis activity	765:797	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	7	31	dep	induced	1234:1240	arg1	acid					1229:1232	the acetic acid	1218:1232	the acetic acid	1218:1232	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	4	32	theme	active	732:737	arg1	component					739:747	the active component	728:747	the active component responsible for anti- ulcerative colitis activity	728:797	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	4	32	theme	active	732:737	arg1	EP-1					630:633	EP-1	630:633	EP-1	630:633	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	8	33	from	expression	1446:1455	arg1	tissues					1468:1474	colonic tissues	1460:1474	colonic tissues	1460:1474	And immunoblot analyses showed that after treated by EP-1, GPR41 and GPR43 were significantly suppressed expression in colonic tissues of the UC rats.					
31059977	1	34	theme	chronic	264:270	arg1	disorder					285:292	an idiopathic, chronic inflammatory disorder	249:292	disorder	285:292	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	1	34	theme	chronic	264:270	arg1	colitis					233:239	Ulcerative colitis	222:239	Ulcerative colitis (UC)	222:244	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	5	35	theme	acetic	924:929	arg1	acid					931:934	acetic acid	924:934	acetic acid	924:934	In this study, our data shows that EP-1 was effective in relieving the symptoms of acetic acid induced UC rats.					
31059977	6	36	theme	colonic	1022:1028	arg1	contents					1030:1037	the rat colonic contents	1014:1037	the rat colonic contents	1014:1037	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	2	37	theme	general	435:441	arg1	population					443:452	the general population	431:452	the general population	431:452	Risk of colorectal cancer in ulcerative colitis is increased in patients with long-standing disease compared with the general population.					
31059977	8	38	theme	UC	1483:1484	arg1	rats					1486:1489	the UC rats	1479:1489	the UC rats	1479:1489	And immunoblot analyses showed that after treated by EP-1, GPR41 and GPR43 were significantly suppressed expression in colonic tissues of the UC rats.					
31059977	4	39	theme	responsible	749:759	arg1	component					739:747	the active component	728:747	the active component responsible for anti- ulcerative colitis activity	728:797	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	4	39	theme	responsible	749:759	arg1	EP-1					630:633	EP-1	630:633	EP-1	630:633	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	5	40	theme	induced	936:942	arg1	rats					947:950	acetic acid induced UC rats	924:950	acetic acid induced UC rats	924:950	In this study, our data shows that EP-1 was effective in relieving the symptoms of acetic acid induced UC rats.					
31059977	3	41	theme	Hericium	455:462	arg1	erinaceus					464:472	Hericium erinaceus	455:472	Hericium erinaceus (HE)	455:477	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	3	41	theme	Hericium	455:462	arg1	HE					475:476	HE	475:476	HE	475:476	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	0	42	theme	Hericium	29:36	arg1	mycelium					48:55	Hericium erinaceus mycelium	29:55	Hericium erinaceus mycelium	29:55	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	0	43	theme	acetic	69:74	arg1	colitis					100:106	acetic acid-induced ulcerative colitis	69:106	acetic acid-induced ulcerative colitis rats	69:111	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	8	44	theme	immunoblot	1345:1354	arg1	analyses					1356:1363	And immunoblot analyses	1341:1363	analyses	1356:1363	And immunoblot analyses showed that after treated by EP-1, GPR41 and GPR43 were significantly suppressed expression in colonic tissues of the UC rats.					
31059977	2	45	theme	cancer	336:341	arg1	Risk					317:320	Risk	317:320	Risk of colorectal cancer in ulcerative colitis	317:363	Risk of colorectal cancer in ulcerative colitis is increased in patients with long-standing disease compared with the general population.					
31059977	1	46	theme	mucosa	309:314	arg1	disorder					285:292	an idiopathic, chronic inflammatory disorder	249:292	disorder	285:292	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	1	46	theme	mucosa	309:314	arg1	colitis					233:239	Ulcerative colitis	222:239	Ulcerative colitis (UC)	222:244	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	9	47	theme	antioxidant	1531:1541	arg1	activities					1583:1592	its antioxidant, anti-inflammatory and enhancing immune activities	1527:1592	its antioxidant, anti-inflammatory and enhancing immune activities	1527:1592	In the meanwhile, EP-1 also showed its antioxidant, anti-inflammatory and enhancing immune activities.					
31059977	7	48	theme	induced	1234:1240	arg1	rats					1245:1248	the acetic acid induced UC rats	1218:1248	the acetic acid induced UC rats	1218:1248	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	9	49	theme	anti-inflammatory	1544:1560	arg1	activities					1583:1592	its antioxidant, anti-inflammatory and enhancing immune activities	1527:1592	its antioxidant, anti-inflammatory and enhancing immune activities	1527:1592	In the meanwhile, EP-1 also showed its antioxidant, anti-inflammatory and enhancing immune activities.					
31059977	7	50	theme	acetic	1222:1227	arg1	acid					1229:1232	the acetic acid	1218:1232	the acetic acid	1218:1232	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	3	51	theme	folk	508:511	arg1	medicine					513:520	traditional folk medicine	496:520	traditional folk medicine	496:520	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	9	52	theme	enhancing	1566:1574	arg1	activities					1583:1592	its antioxidant, anti-inflammatory and enhancing immune activities	1527:1592	its antioxidant, anti-inflammatory and enhancing immune activities	1527:1592	In the meanwhile, EP-1 also showed its antioxidant, anti-inflammatory and enhancing immune activities.					
31059977	6	53	theme	flora	1069:1073	arg1	structure					1075:1083	the intestinal flora structure	1054:1083	the intestinal flora structure	1054:1083	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	0	54	theme	acids	184:188	arg1	levels					190:195	short chain fatty acids levels	166:195	short chain fatty acids levels	166:195	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	2	55	theme	ulcerative	346:355	arg1	colitis					357:363	ulcerative colitis	346:363	ulcerative colitis	346:363	Risk of colorectal cancer in ulcerative colitis is increased in patients with long-standing disease compared with the general population.					
31059977	3	56	used	used	488:491	arg2	HE					475:476	HE	475:476	HE	475:476	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	3	56	used	used	488:491	arg2	erinaceus					464:472	Hericium erinaceus	455:472	Hericium erinaceus (HE)	455:477	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	10	57	theme	alternative	1701:1711	arg1	therapy					1733:1739	the complementary and alternative medicine (CAM) herb therapy	1679:1739	therapy	1733:1739	Thus, the polysaccharide purified from HE showed potential for anti-UC activity and the complementary and alternative medicine (CAM) herb therapy.					
31059977	1	58	theme	idiopathic	252:261	arg1	disorder					285:292	an idiopathic, chronic inflammatory disorder	249:292	disorder	285:292	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	1	58	theme	idiopathic	252:261	arg1	colitis					233:239	Ulcerative colitis	222:239	Ulcerative colitis (UC)	222:244	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	0	59	theme	respectors	210:219	arg1	composition					131:141	the composition	127:141	the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors	127:219	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	3	60	from	medicine	513:520	arg1	Japan					564:568	Japan	564:568	Japan	564:568	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	3	60	from	medicine	513:520	arg1	Korea					554:558	Korea	554:558	Korea	554:558	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	3	60	from	medicine	513:520	arg1	China					547:551	China	547:551	China	547:551	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	4	61	theme	unique	647:652	arg1	polysaccharide					654:667	a purified unique polysaccharide	636:667	a purified unique polysaccharide isolated from HE mycelium	636:693	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	4	61	theme	unique	647:652	arg1	EP-1					630:633	EP-1	630:633	EP-1	630:633	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	6	62	theme	model	1111:1115	arg1	rats					1117:1120	the model rats	1107:1120	the model rats	1107:1120	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	0	63	theme	gut	150:152	arg1	microbiota					154:163	gut microbiota	150:163	gut microbiota	150:163	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	3	64	theme	anti-gastritis	575:588	arg1	activities					618:627	anti-gastritis and anti-ulcerative colitis activities	575:627	anti-gastritis and anti-ulcerative colitis activities	575:627	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	0	65	theme	chain	172:176	arg1	acids					184:188	short chain fatty acids	166:188	short chain fatty acids levels	166:195	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	6	66	theme	rRNA	995:998	arg1	sequencing					1000:1009	16S rRNA sequencing	991:1009	16S rRNA sequencing of the rat colonic contents	991:1037	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	7	67	theme	chain	1314:1318	arg1	acids					1326:1330	short chain fatty acids	1308:1330	short chain fatty acids (SCFAs)	1308:1338	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	7	67	theme	chain	1314:1318	arg1	SCFAs					1333:1337	SCFAs	1333:1337	SCFAs	1333:1337	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	0	68	theme	acid-induced	76:87	arg1	colitis					100:106	acetic acid-induced ulcerative colitis	69:106	acetic acid-induced ulcerative colitis rats	69:111	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	3	69	theme	anti-ulcerative	594:608	arg1	colitis					610:616	anti-ulcerative colitis	594:616	anti-ulcerative colitis	594:616	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	5	70	dep	induced	936:942	arg1	acid					931:934	acetic acid	924:934	acetic acid	924:934	In this study, our data shows that EP-1 was effective in relieving the symptoms of acetic acid induced UC rats.					
31059977	6	71	theme	MiSeq	975:979	arg1	platform					981:988	the Illumina MiSeq platform	962:988	the Illumina MiSeq platform	962:988	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	3	72	from	cuisine	536:542	arg1	Japan					564:568	Japan	564:568	Japan	564:568	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	3	72	from	cuisine	536:542	arg1	Korea					554:558	Korea	554:558	Korea	554:558	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	3	72	from	cuisine	536:542	arg1	China					547:551	China	547:551	China	547:551	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	5	73	theme	UC	944:945	arg1	rats					947:950	acetic acid induced UC rats	924:950	acetic acid induced UC rats	924:950	In this study, our data shows that EP-1 was effective in relieving the symptoms of acetic acid induced UC rats.					
31059977	7	74	theme	microbiota	1273:1282	arg1	community					1284:1292	the gut microbiota community	1265:1292	the gut microbiota community	1265:1292	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	4	75	theme	ulcerative	771:780	arg1	colitis					782:788	anti- ulcerative colitis	765:788	anti- ulcerative colitis activity	765:797	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	0	76	theme	unique	2:7	arg1	polysaccharide					9:22	A unique polysaccharide	0:22	A unique polysaccharide from Hericium erinaceus mycelium	0:55	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	0	77	dep	colitis	100:106	arg1	rats					108:111	rats	108:111	acetic acid-induced ulcerative colitis rats	69:111	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	8	78	theme	colonic	1460:1466	arg1	tissues					1468:1474	colonic tissues	1460:1474	colonic tissues	1460:1474	And immunoblot analyses showed that after treated by EP-1, GPR41 and GPR43 were significantly suppressed expression in colonic tissues of the UC rats.					
31059977	6	79	theme	contents	1030:1037	arg1	sequencing					1000:1009	16S rRNA sequencing	991:1009	16S rRNA sequencing of the rat colonic contents	991:1037	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	8	80	theme	rats	1486:1489	arg1	expression					1446:1455	expression	1446:1455	expression in colonic tissues of the UC rats	1446:1489	And immunoblot analyses showed that after treated by EP-1, GPR41 and GPR43 were significantly suppressed expression in colonic tissues of the UC rats.					
31059977	1	81	theme	inflammatory	272:283	arg1	disorder					285:292	an idiopathic, chronic inflammatory disorder	249:292	disorder	285:292	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	1	81	theme	inflammatory	272:283	arg1	colitis					233:239	Ulcerative colitis	222:239	Ulcerative colitis (UC)	222:244	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	6	82	theme	rat	1018:1020	arg1	contents					1030:1037	the rat colonic contents	1014:1037	the rat colonic contents	1014:1037	Based on the Illumina MiSeq platform, 16S rRNA sequencing of the rat colonic contents indicated that the intestinal flora structure remarkably changed in the model rats and the tendency was alleviated to a certain degree by EP-1.					
31059977	0	83	theme	erinaceus	38:46	arg1	mycelium					48:55	Hericium erinaceus mycelium	29:55	Hericium erinaceus mycelium	29:55	A unique polysaccharide from Hericium erinaceus mycelium ameliorates acetic acid-induced ulcerative colitis rats by modulating the composition of the gut microbiota, short chain fatty acids levels and GPR41/43 respectors.					
31059977	7	84	theme	UC	1242:1243	arg1	rats					1245:1248	the acetic acid induced UC rats	1218:1248	the acetic acid induced UC rats	1218:1248	The further results showed that in the acetic acid induced UC rats EP-1 modulated the gut microbiota community and increased short chain fatty acids (SCFAs).					
31059977	4	85	theme	cell	810:813	arg1	model					815:819	a cell model	808:819	a cell model for identification	808:838	EP-1, a purified unique polysaccharide isolated from HE mycelium, has recently been identified as the active component responsible for anti- ulcerative colitis activity by using a cell model for identification.					
31059977	2	86	theme	long-standing	395:407	arg1	disease					409:415	long-standing disease	395:415	long-standing disease	395:415	Risk of colorectal cancer in ulcerative colitis is increased in patients with long-standing disease compared with the general population.					
31059977	3	87	with	cuisine	536:542	arg1	activities					618:627	anti-gastritis and anti-ulcerative colitis activities	575:627	anti-gastritis and anti-ulcerative colitis activities	575:627	Hericium erinaceus (HE) has been used in traditional folk medicine and medicinal cuisine in China, Korea and Japan with anti-gastritis and anti-ulcerative colitis activities.					
31059977	1	88	theme	colonic	301:307	arg1	mucosa					309:314	the colonic mucosa	297:314	the colonic mucosa	297:314	Ulcerative colitis (UC) is an idiopathic, chronic inflammatory disorder of the colonic mucosa.					
31059977	2	89	theme	colorectal	325:334	arg1	cancer					336:341	colorectal cancer	325:341	colorectal cancer	325:341	Risk of colorectal cancer in ulcerative colitis is increased in patients with long-standing disease compared with the general population.					
30786159	2	0	theme	organic/inorganic	480:496	arg1	scaffolds					498:506	organic/inorganic scaffolds	480:506	organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing	480:585	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	0	1	from	architecture	26:37	arg1	glass					61:65	chitosan/bioactive glass	42:65	chitosan/bioactive glass	42:65	Hierarchical microchannel architecture in chitosan/bioactive glass scaffolds via electrophoretic deposition positive-replica.					
30786159	8	2	theme	new	1561:1563	arg1	generation					1565:1574	a new generation	1559:1574	a new generation of bone scaffolds	1559:1592	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	5	3	theme	In	929:930	arg1	analysis					938:945	In vitro analysis	929:945	In vitro analysis in simulated body fluid (SBF)	929:975	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	7	4	theme	scaffold	1364:1371	arg1	colonization					1373:1384	scaffold colonization	1364:1384	scaffold colonization	1364:1384	The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization.					
30786159	7	4	theme	scaffold	1364:1371	arg1	cells					1329:1333	cells proliferation, viability and scaffold colonization	1329:1384	cells proliferation, viability and scaffold colonization	1329:1384	The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization.					
30786159	1	5	theme	native	275:280	arg1	tissue					282:287	the native tissue	271:287	the native tissue	271:287	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	5	6	theme	smaller	1131:1137	arg1	particles					1142:1150	smaller BG particles	1131:1150	smaller BG particles	1131:1150	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	3	7	theme	chitosan	687:694	arg1	matrix					696:701	a chitosan matrix	685:701	a chitosan matrix	685:701	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	1	8	theme	cortical	184:191	arg1	regeneration					198:209	cortical bone regeneration	184:209	cortical bone regeneration	184:209	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	1	9	theme	tissue	282:287	arg1	structure					258:266	the highly oriented, hierarchical structure	224:266	the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way	224:331	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	2	10	theme	Haversian	549:557	arg1	canals					559:564	Haversian canals diameter and spacing	549:585	Haversian canals diameter and spacing	549:585	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	2	10	theme	Haversian	549:557	arg1	spacing					579:585	spacing	579:585	spacing	579:585	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	2	10	theme	Haversian	549:557	arg1	diameter					566:573	diameter	566:573	diameter	566:573	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	8	11	theme	bone	1579:1582	arg1	scaffolds					1584:1592	bone scaffolds	1579:1592	bone scaffolds	1579:1592	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	10	12	dep	A	1650:1650	arg1	Res					1641:1643	Res	1641:1643	Res	1641:1643	J Biomed Mater Res Part A, 2019.					
30786159	6	13	theme	hybrid	1175:1180	arg1	scaffolds					1182:1190	hybrid scaffolds	1175:1190	hybrid scaffolds	1175:1190	Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS-2).					
30786159	6	14	theme	bone	1220:1223	arg1	cells					1238:1242	bone osteosarcoma cells	1220:1242	bone osteosarcoma cells (SAOS-2)	1220:1251	Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS-2).					
30786159	6	14	theme	bone	1220:1223	arg1	SAOS-2					1245:1250	SAOS-2	1245:1250	SAOS-2	1245:1250	Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS-2).					
30786159	6	15	with	interaction	1158:1168	arg1	scaffolds					1182:1190	hybrid scaffolds	1175:1190	hybrid scaffolds	1175:1190	Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS-2).					
30786159	2	16	dep	canals	559:564	arg1	canals					559:564	Haversian canals diameter and spacing	549:585	Haversian canals diameter and spacing	549:585	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	2	16	dep	canals	559:564	arg1	spacing					579:585	spacing	579:585	spacing	579:585	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	2	16	dep	canals	559:564	arg1	diameter					566:573	diameter	566:573	diameter	566:573	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	9	17	dep	©	1595:1595	arg1	Inc.					1621:1624	Inc.	1621:1624	Inc.	1621:1624	© 2019 Wiley Periodicals, Inc.					
30786159	8	18	theme	scaffolds	1584:1592	arg1	generation					1565:1574	a new generation	1559:1574	a new generation of bone scaffolds	1559:1592	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	7	19	theme	EPD	1287:1289	arg1	structures					1291:1300	EPD structures	1287:1300	EPD structures	1287:1300	The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization.					
30786159	2	20	with	scaffolds	498:506	arg1	micro-porosities					522:537	oriented micro-porosities	513:537	oriented micro-porosities mimicking Haversian canals diameter and spacing	513:585	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	1	21	theme	bone	193:196	arg1	regeneration					198:209	cortical bone regeneration	184:209	cortical bone regeneration	184:209	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	2	22	theme	oriented	513:520	arg1	micro-porosities					522:537	oriented micro-porosities	513:537	oriented micro-porosities mimicking Haversian canals diameter and spacing	513:585	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	8	23	theme	generation	1565:1574	arg1	development					1544:1554	the development	1540:1554	the development of a new generation of bone scaffolds	1540:1592	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	4	24	theme	homogeneous	781:791	arg1	dispersion					793:802	a homogeneous dispersion	779:802	a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm)	779:911	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	0	25	theme	microchannel	13:24	arg1	architecture					26:37	Hierarchical microchannel architecture	0:37	Hierarchical microchannel architecture in chitosan/bioactive glass	0:65	Hierarchical microchannel architecture in chitosan/bioactive glass scaffolds via electrophoretic deposition positive-replica.					
30786159	3	26	theme	micro-patterned	714:728	arg1	cathodes					730:737	micro-patterned cathodes	714:737	micro-patterned cathodes	714:737	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	4	27	theme	spacing = 600 ± 40	890:907	arg1	μm					909:910	inter-channel spacing = 600 ± 40 μm	876:910	inter-channel spacing = 600 ± 40 μm	876:910	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	7	28	theme	osteoconductive	1258:1272	arg1	potential					1274:1282	The osteoconductive potential	1254:1282	The osteoconductive potential of EPD structures	1254:1300	The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization.					
30786159	3	29	theme	different	592:600	arg1	inclusions					660:669	inclusions	660:669	inclusions	660:669	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	3	29	theme	different	592:600	arg1	sizes					602:606	Two different sizes	588:606	Two different sizes of 45S5 bioactive glass (BG) powders	588:643	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	4	30	theme	chitosan	754:761	arg1	scaffolds					763:771	Self-standing chitosan scaffolds	740:771	Self-standing chitosan scaffolds	740:771	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	0	31	theme	Hierarchical	0:11	arg1	architecture					26:37	Hierarchical microchannel architecture	0:37	Hierarchical microchannel architecture in chitosan/bioactive glass	0:65	Hierarchical microchannel architecture in chitosan/bioactive glass scaffolds via electrophoretic deposition positive-replica.					
30786159	1	32	theme	efficient	295:303	arg1	way					329:331	an efficient, simple, and consistent way	292:331	an efficient, simple, and consistent way	292:331	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	4	33	theme	inter-channel	876:888	arg1	μm					909:910	inter-channel spacing = 600 ± 40 μm	876:910	inter-channel spacing = 600 ± 40 μm	876:910	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	1	34	from	challenges	142:151	arg1	design					160:165	the design	156:165	the design of scaffolds for cortical bone regeneration	156:209	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	4	35	theme	Self-standing	740:752	arg1	scaffolds					763:771	Self-standing chitosan scaffolds	740:771	Self-standing chitosan scaffolds	740:771	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	5	36	theme	BG	1139:1140	arg1	particles					1142:1150	smaller BG particles	1131:1150	smaller BG particles	1131:1150	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	2	37	theme	positive	376:383	arg1	solution					348:355	a possible solution	337:355	a possible solution to this challenge	337:373	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	2	37	theme	positive	376:383	arg1	technique					459:467	a technique	457:467	a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing	457:585	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	2	37	theme	positive	376:383	arg1	replica					385:391	positive replica	376:391	positive replica based on electrophoretic deposition (EPD)	376:433	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	5	38	from	analysis	938:945	arg1	SBF					972:974	SBF	972:974	SBF	972:974	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	5	38	from	analysis	938:945	arg1	fluid					965:969	simulated body fluid	950:969	simulated body fluid (SBF)	950:975	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	8	39	theme	micro-patterned	1480:1494	arg1	structures					1496:1505	composite micro-patterned structures	1470:1505	composite micro-patterned structures	1470:1505	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	6	40	theme	osteosarcoma	1225:1236	arg1	cells					1238:1242	bone osteosarcoma cells	1220:1242	bone osteosarcoma cells (SAOS-2)	1220:1251	Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS-2).					
30786159	6	40	theme	osteosarcoma	1225:1236	arg1	SAOS-2					1245:1250	SAOS-2	1245:1250	SAOS-2	1245:1250	Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS-2).					
30786159	6	41	theme	Cell	1153:1156	arg1	interaction					1158:1168	Cell interaction	1153:1168	Cell interaction with hybrid scaffolds	1153:1190	Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS-2).					
30786159	4	42	theme	micro-channels	843:856	arg1	dispersion					793:802	a homogeneous dispersion	779:802	a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm)	779:911	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	4	43	dep	= 380 ± 50 μm	861:873	arg1	μm					909:910	inter-channel spacing = 600 ± 40 μm	876:910	inter-channel spacing = 600 ± 40 μm	876:910	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	1	44	theme	simple	306:311	arg1	way					329:331	an efficient, simple, and consistent way	292:331	an efficient, simple, and consistent way	292:331	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	5	45	dep	In	929:930	arg1	vitro					932:936	vitro	932:936	vitro	932:936	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	1	46	theme	main	137:140	arg1	challenges					142:151	the main challenges	133:151	the main challenges in the design of scaffolds for cortical bone regeneration	133:209	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	5	47	dep	appearing	1098:1106	arg1	favored					1120:1126	favored	1120:1126	appearing kinetically favored by smaller BG particles	1098:1150	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	3	48	theme	bioactive	616:624	arg1	powders					637:643	45S5 bioactive glass (BG) powders	611:643	45S5 bioactive glass (BG) powders	611:643	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	1	49	theme	oriented	235:242	arg1	structure					258:266	the highly oriented, hierarchical structure	224:266	the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way	224:331	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	0	50	theme	chitosan/bioactive	42:59	arg1	glass					61:65	chitosan/bioactive glass	42:65	chitosan/bioactive glass	42:65	Hierarchical microchannel architecture in chitosan/bioactive glass scaffolds via electrophoretic deposition positive-replica.					
30786159	10	51	theme	Part	1645:1648	arg1	2019					1653:1656	2019	1653:1656	2019	1653:1656	J Biomed Mater Res Part A, 2019.					
30786159	10	51	theme	Part	1645:1648	arg1	A					1650:1650	Part A	1645:1650	Part A	1645:1650	J Biomed Mater Res Part A, 2019.					
30786159	5	52	theme	Ca/P	1072:1075	arg1	reactions					1088:1096	Ca/P nucleation reactions	1072:1096	Ca/P nucleation reactions appearing kinetically favored by smaller BG particles	1072:1150	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	3	53	theme	glass	626:630	arg1	powders					637:643	45S5 bioactive glass (BG) powders	611:643	45S5 bioactive glass (BG) powders	611:643	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	8	54	theme	composite	1470:1478	arg1	structures					1496:1505	composite micro-patterned structures	1470:1505	composite micro-patterned structures	1470:1505	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	2	55	theme	electrophoretic	402:416	arg1	deposition					418:427	electrophoretic deposition	402:427	electrophoretic deposition (EPD)	402:433	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	2	55	theme	electrophoretic	402:416	arg1	EPD					430:432	EPD	430:432	EPD	430:432	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	1	56	theme	consistent	318:327	arg1	way					329:331	an efficient, simple, and consistent way	292:331	an efficient, simple, and consistent way	292:331	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	5	57	theme	nucleation	1077:1086	arg1	reactions					1088:1096	Ca/P nucleation reactions	1072:1096	Ca/P nucleation reactions appearing kinetically favored by smaller BG particles	1072:1150	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	5	58	theme	layer	1037:1041	arg1	deposition					1010:1019	a deposition	1008:1019	a deposition of a homogenous layer of hydroxyapatite (HA)	1008:1064	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	1	59	theme	challenges	142:151	arg1	challenges					142:151	the main challenges	133:151	the main challenges in the design of scaffolds for cortical bone regeneration	133:209	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	1	59	theme	challenges	142:151	arg1	One					126:128	One	126:128	One	126:128	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	5	60	theme	hydroxyapatite	1046:1059	arg1	layer					1037:1041	a homogenous layer	1024:1041	a homogenous layer of hydroxyapatite (HA)	1024:1064	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	3	61	theme	45S5	611:614	arg1	powders					637:643	45S5 bioactive glass (BG) powders	611:643	45S5 bioactive glass (BG) powders	611:643	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	7	62	dep	cells	1329:1333	arg1	colonization					1373:1384	scaffold colonization	1364:1384	scaffold colonization	1364:1384	The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization.					
30786159	7	62	dep	cells	1329:1333	arg1	proliferation					1335:1347	proliferation	1335:1347	proliferation	1335:1347	The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization.					
30786159	7	62	dep	cells	1329:1333	arg1	cells					1329:1333	cells proliferation, viability and scaffold colonization	1329:1384	cells proliferation, viability and scaffold colonization	1329:1384	The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization.					
30786159	7	62	dep	cells	1329:1333	arg1	viability					1350:1358	viability	1350:1358	viability	1350:1358	The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization.					
30786159	2	63	theme	possible	339:346	arg1	solution					348:355	a possible solution	337:355	a possible solution to this challenge	337:373	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	2	63	theme	possible	339:346	arg1	replica					385:391	positive replica	376:391	positive replica based on electrophoretic deposition (EPD)	376:433	As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing.					
30786159	8	64	theme	effective	1439:1447	arg1	technique					1449:1457	a simple yet extremely effective technique	1416:1457	a simple yet extremely effective technique to prepare composite micro-patterned structures	1416:1505	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	8	64	theme	effective	1439:1447	arg1	EPD					1409:1411	EPD	1409:1411	EPD	1409:1411	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	4	65	theme	particles	810:818	arg1	dispersion					793:802	a homogeneous dispersion	779:802	a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm)	779:911	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	5	66	theme	homogenous	1026:1035	arg1	layer					1037:1041	a homogenous layer	1024:1041	a homogenous layer of hydroxyapatite (HA)	1024:1064	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	4	67	theme	BG	807:808	arg1	particles					810:818	BG particles	807:818	BG particles	807:818	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	4	67	theme	BG	807:808	arg1	= 380 ± 50 μm					861:873	ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm	859:910	= 380 ± 50 μm	861:873	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	1	68	dep	oriented	235:242	arg1	hierarchical					245:256	hierarchical	245:256	hierarchical	245:256	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	4	69	theme	regularly-oriented	824:841	arg1	micro-channels					843:856	regularly-oriented micro-channels	824:856	regularly-oriented micro-channels	824:856	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
30786159	5	70	theme	simulated	950:958	arg1	SBF					972:974	SBF	972:974	SBF	972:974	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	5	70	theme	simulated	950:958	arg1	fluid					965:969	simulated body fluid	950:969	simulated body fluid (SBF)	950:975	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	1	71	from	structure	258:266	arg1	way					329:331	an efficient, simple, and consistent way	292:331	an efficient, simple, and consistent way	292:331	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	7	72	theme	structures	1291:1300	arg1	potential					1274:1282	The osteoconductive potential	1254:1282	The osteoconductive potential of EPD structures	1254:1300	The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization.					
30786159	3	73	theme	powders	637:643	arg1	inclusions					660:669	inclusions	660:669	inclusions	660:669	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	3	73	theme	powders	637:643	arg1	sizes					602:606	Two different sizes	588:606	Two different sizes of 45S5 bioactive glass (BG) powders	588:643	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	5	74	theme	body	960:963	arg1	SBF					972:974	SBF	972:974	SBF	972:974	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	5	74	theme	body	960:963	arg1	fluid					965:969	simulated body fluid	950:969	simulated body fluid (SBF)	950:975	In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles.					
30786159	0	75	theme	deposition	97:106	arg1	positive-replica					108:123	electrophoretic deposition positive-replica	81:123	electrophoretic deposition positive-replica	81:123	Hierarchical microchannel architecture in chitosan/bioactive glass scaffolds via electrophoretic deposition positive-replica.					
30786159	8	76	theme	simple	1418:1423	arg1	technique					1449:1457	a simple yet extremely effective technique	1416:1457	a simple yet extremely effective technique to prepare composite micro-patterned structures	1416:1505	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	8	76	theme	simple	1418:1423	arg1	EPD					1409:1411	EPD	1409:1411	EPD	1409:1411	Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds.					
30786159	0	77	theme	electrophoretic	81:95	arg1	positive-replica					108:123	electrophoretic deposition positive-replica	81:123	electrophoretic deposition positive-replica	81:123	Hierarchical microchannel architecture in chitosan/bioactive glass scaffolds via electrophoretic deposition positive-replica.					
30786159	3	78	theme	BG	633:634	arg1	powders					637:643	45S5 bioactive glass (BG) powders	611:643	45S5 bioactive glass (BG) powders	611:643	Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes.					
30786159	1	79	theme	scaffolds	170:178	arg1	design					160:165	the design	156:165	the design of scaffolds for cortical bone regeneration	156:209	One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way.					
30786159	4	80	with	scaffolds	763:771	arg1	dispersion					793:802	a homogeneous dispersion	779:802	a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm)	779:911	Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained.					
31472861	0	0	theme	nanofibrillar	80:92	arg1	cellulose					104:112	porous 3D nanofibrillar bacterial cellulose	70:112	porous 3D nanofibrillar bacterial cellulose	70:112	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.					
31472861	2	1	theme	nanofibrillar	482:494	arg1	BC					496:497	the nanofibrillar BC	478:497	the nanofibrillar BC	478:497	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
31472861	4	2	theme	resulting	669:677	arg1	aerogels					686:693	the resulting BC-QDs aerogels	665:693	the resulting BC-QDs aerogels	665:693	After supercritical CO2 drying, the resulting BC-QDs aerogels maintain the porous nanofibrillar morphology of BC with ultra-light-weight, the QDs are well-distributed along the BC fiber surfaces without aggregation.					
31472861	1	3	theme	nanofibrillar	151:163	arg1	structure					173:181	a nanofibrillar network structure	149:181	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.	115:314	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	0	4	theme	3D	77:78	arg1	cellulose					104:112	porous 3D nanofibrillar bacterial cellulose	70:112	porous 3D nanofibrillar bacterial cellulose	70:112	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.					
31472861	1	5	theme	network	165:171	arg1	structure					173:181	a nanofibrillar network structure	149:181	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.	115:314	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	0	6	theme	bacterial	94:102	arg1	cellulose					104:112	porous 3D nanofibrillar bacterial cellulose	70:112	porous 3D nanofibrillar bacterial cellulose	70:112	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.					
31472861	4	7	theme	BC	743:744	arg1	morphology					729:738	the porous nanofibrillar morphology	704:738	the porous nanofibrillar morphology of BC with ultra-light-weight	704:768	After supercritical CO2 drying, the resulting BC-QDs aerogels maintain the porous nanofibrillar morphology of BC with ultra-light-weight, the QDs are well-distributed along the BC fiber surfaces without aggregation.					
31472861	2	8	theme	novel	336:340	arg1	aerogels					354:361	novel fluorescent aerogels	336:361	novel fluorescent aerogels	336:361	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
31472861	5	9	theme	wide	891:894	arg1	range					896:900	a wide range	889:900	a wide range from 470 to 750 nm	889:919	The emission wavelength can be tuned in a wide range from 470 to 750 nm by simply adjusting the QDs core component or shell layers.					
31472861	1	10	from	quenching-effect	269:284	arg1	composites					304:313	polymer composites	296:313	polymer composites	296:313	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	2	11	theme	environmentally-friendly	407:430	arg1	dots					467:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
31472861	3	12	theme	3-	516:517	arg1	trimethoxysilane					534:549	3-(mercaptopropyl)trimethoxysilane	516:549	3-(mercaptopropyl)trimethoxysilane	516:549	A new ligand of 3-(mercaptopropyl)trimethoxysilane allows QDs to transfer from toluene to alcohol solvent and stably bind to the BC.					
31472861	4	13	theme	CO2	653:655	arg1	drying					657:662	supercritical CO2 drying	639:662	supercritical CO2 drying	639:662	After supercritical CO2 drying, the resulting BC-QDs aerogels maintain the porous nanofibrillar morphology of BC with ultra-light-weight, the QDs are well-distributed along the BC fiber surfaces without aggregation.					
31472861	5	14	dep	750 nm	914:919	arg1	to					911:912	to	911:912	to	911:912	The emission wavelength can be tuned in a wide range from 470 to 750 nm by simply adjusting the QDs core component or shell layers.					
31472861	5	15	theme	core	949:952	arg1	component					954:962	the QDs core component or shell layers	941:978	component	954:962	The emission wavelength can be tuned in a wide range from 470 to 750 nm by simply adjusting the QDs core component or shell layers.					
31472861	5	16	theme	emission	853:860	arg1	wavelength					862:871	The emission wavelength	849:871	The emission wavelength	849:871	The emission wavelength can be tuned in a wide range from 470 to 750 nm by simply adjusting the QDs core component or shell layers.					
31472861	2	17	theme	/ZnS	443:446	arg1	dots					467:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
31472861	6	18	theme	smart	1131:1135	arg1	sensor					1137:1142	smart sensor	1131:1142	smart sensor	1131:1142	This work provides a new approach for fabricating QDs-polymer hydrogels and aerogels with well distributed QDs via chemical binding that potential as smart sensor, catalysis, and 3D display applications.					
31472861	5	19	theme	shell	967:971	arg1	layers					973:978	the QDs core component or shell layers	941:978	layers	973:978	The emission wavelength can be tuned in a wide range from 470 to 750 nm by simply adjusting the QDs core component or shell layers.					
31472861	4	20	theme	nanofibrillar	715:727	arg1	morphology					729:738	the porous nanofibrillar morphology	704:738	the porous nanofibrillar morphology of BC with ultra-light-weight	704:768	After supercritical CO2 drying, the resulting BC-QDs aerogels maintain the porous nanofibrillar morphology of BC with ultra-light-weight, the QDs are well-distributed along the BC fiber surfaces without aggregation.					
31472861	6	21	with	aerogels	1057:1064	arg1	QDs					1088:1090	well distributed QDs	1071:1090	well distributed QDs	1071:1090	This work provides a new approach for fabricating QDs-polymer hydrogels and aerogels with well distributed QDs via chemical binding that potential as smart sensor, catalysis, and 3D display applications.					
31472861	2	22	theme	tunable	368:374	arg1	emission					376:383	tunable emission	368:383	tunable emission	368:383	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
31472861	0	23	theme	ZCIS/ZnS	0:7	arg1	aerogels					25:32	ZCIS/ZnS QDs fluorescent aerogels	0:32	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.	0:113	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.					
31472861	4	24	theme	porous	708:713	arg1	morphology					729:738	the porous nanofibrillar morphology	704:738	the porous nanofibrillar morphology of BC with ultra-light-weight	704:768	After supercritical CO2 drying, the resulting BC-QDs aerogels maintain the porous nanofibrillar morphology of BC with ultra-light-weight, the QDs are well-distributed along the BC fiber surfaces without aggregation.					
31472861	4	25	theme	supercritical	639:651	arg1	drying					657:662	supercritical CO2 drying	639:662	supercritical CO2 drying	639:662	After supercritical CO2 drying, the resulting BC-QDs aerogels maintain the porous nanofibrillar morphology of BC with ultra-light-weight, the QDs are well-distributed along the BC fiber surfaces without aggregation.					
31472861	2	26	theme	CuInS2	436:441	arg1	dots					467:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
31472861	1	27	theme	fluorescence	256:267	arg1	quenching-effect					269:284	the fluorescence quenching-effect	252:284	the fluorescence quenching-effect of QDs in polymer composites	252:313	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	1	28	dep	features	140:147	arg1	structure					173:181	a nanofibrillar network structure	149:181	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.	115:314	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	2	29	theme	ZnS	432:434	arg1	dots					467:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
31472861	0	30	theme	fluorescent	13:23	arg1	aerogels					25:32	ZCIS/ZnS QDs fluorescent aerogels	0:32	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.	0:113	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.					
31472861	1	31	theme	Bacterial	115:123	arg1	BC					136:137	BC	136:137	BC	136:137	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	1	31	theme	Bacterial	115:123	arg1	cellulose					125:133	Bacterial cellulose	115:133	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.	115:314	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	1	32	theme	good	202:205	arg1	template					207:214	a good template	200:214	a good template for quantum dots (QDs)	200:237	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	0	33	theme	tunable	39:45	arg1	emission					47:54	tunable emission	39:54	tunable emission prepared from porous 3D nanofibrillar bacterial cellulose	39:112	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.					
31472861	6	34	theme	new	1002:1004	arg1	approach					1006:1013	a new approach	1000:1013	a new approach for fabricating QDs-polymer hydrogels and aerogels with well distributed QDs via chemical binding that potential as smart sensor, catalysis, and 3D display applications	1000:1182	This work provides a new approach for fabricating QDs-polymer hydrogels and aerogels with well distributed QDs via chemical binding that potential as smart sensor, catalysis, and 3D display applications.					
31472861	1	35	theme	QDs	289:291	arg1	quenching-effect					269:284	the fluorescence quenching-effect	252:284	the fluorescence quenching-effect of QDs in polymer composites	252:313	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	0	36	with	aerogels	25:32	arg1	emission					47:54	tunable emission	39:54	tunable emission prepared from porous 3D nanofibrillar bacterial cellulose	39:112	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.					
31472861	6	37	theme	QDs-polymer	1031:1041	arg1	hydrogels					1043:1051	QDs-polymer hydrogels	1031:1051	QDs-polymer hydrogels	1031:1051	This work provides a new approach for fabricating QDs-polymer hydrogels and aerogels with well distributed QDs via chemical binding that potential as smart sensor, catalysis, and 3D display applications.					
31472861	2	38	theme	quantum	459:465	arg1	dots					467:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
31472861	4	39	with	morphology	729:738	arg1	ultra-light-weight					751:768	ultra-light-weight	751:768	ultra-light-weight	751:768	After supercritical CO2 drying, the resulting BC-QDs aerogels maintain the porous nanofibrillar morphology of BC with ultra-light-weight, the QDs are well-distributed along the BC fiber surfaces without aggregation.					
31472861	6	40	with	hydrogels	1043:1051	arg1	QDs					1088:1090	well distributed QDs	1071:1090	well distributed QDs	1071:1090	This work provides a new approach for fabricating QDs-polymer hydrogels and aerogels with well distributed QDs via chemical binding that potential as smart sensor, catalysis, and 3D display applications.					
31472861	4	41	theme	fiber	813:817	arg1	surfaces					819:826	the BC fiber surfaces	806:826	the BC fiber surfaces without aggregation	806:846	After supercritical CO2 drying, the resulting BC-QDs aerogels maintain the porous nanofibrillar morphology of BC with ultra-light-weight, the QDs are well-distributed along the BC fiber surfaces without aggregation.					
31472861	1	42	theme	cellulose	125:133	arg1	features					140:147	Bacterial cellulose (BC) features	115:147	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.	115:314	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	2	43	theme	core-shell	448:457	arg1	dots					467:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots	407:470	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
31472861	1	44	theme	polymer	296:302	arg1	composites					304:313	polymer composites	296:313	polymer composites	296:313	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	4	45	theme	BC	810:811	arg1	surfaces					819:826	the BC fiber surfaces	806:826	the BC fiber surfaces without aggregation	806:846	After supercritical CO2 drying, the resulting BC-QDs aerogels maintain the porous nanofibrillar morphology of BC with ultra-light-weight, the QDs are well-distributed along the BC fiber surfaces without aggregation.					
31472861	3	46	theme	new	502:504	arg1	ligand					506:511	A new ligand	500:511	A new ligand of 3-(mercaptopropyl)trimethoxysilane	500:549	A new ligand of 3-(mercaptopropyl)trimethoxysilane allows QDs to transfer from toluene to alcohol solvent and stably bind to the BC.					
31472861	6	47	theme	chemical	1096:1103	arg1	binding					1105:1111	chemical binding	1096:1111	chemical binding	1096:1111	This work provides a new approach for fabricating QDs-polymer hydrogels and aerogels with well distributed QDs via chemical binding that potential as smart sensor, catalysis, and 3D display applications.					
31472861	3	48	theme	trimethoxysilane	534:549	arg1	ligand					506:511	A new ligand	500:511	A new ligand of 3-(mercaptopropyl)trimethoxysilane	500:549	A new ligand of 3-(mercaptopropyl)trimethoxysilane allows QDs to transfer from toluene to alcohol solvent and stably bind to the BC.					
31472861	1	49	theme	quantum	220:226	arg1	QDs					234:236	QDs	234:236	QDs	234:236	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	1	49	theme	quantum	220:226	arg1	dots					228:231	quantum dots	220:231	quantum dots (QDs)	220:237	Bacterial cellulose (BC) features a nanofibrillar network structure that can provide a good template for quantum dots (QDs), to overcome the fluorescence quenching-effect of QDs in polymer composites.					
31472861	0	50	theme	porous	70:75	arg1	cellulose					104:112	porous 3D nanofibrillar bacterial cellulose	70:112	porous 3D nanofibrillar bacterial cellulose	70:112	ZCIS/ZnS QDs fluorescent aerogels with tunable emission prepared from porous 3D nanofibrillar bacterial cellulose.					
31472861	3	51	theme	alcohol	590:596	arg1	solvent					598:604	alcohol solvent	590:604	alcohol solvent	590:604	A new ligand of 3-(mercaptopropyl)trimethoxysilane allows QDs to transfer from toluene to alcohol solvent and stably bind to the BC.					
31472861	6	52	theme	distributed	1076:1086	arg1	QDs					1088:1090	well distributed QDs	1071:1090	well distributed QDs	1071:1090	This work provides a new approach for fabricating QDs-polymer hydrogels and aerogels with well distributed QDs via chemical binding that potential as smart sensor, catalysis, and 3D display applications.					
31472861	5	53	from	750 nm	914:919	arg1	range					896:900	a wide range	889:900	a wide range from 470 to 750 nm	889:919	The emission wavelength can be tuned in a wide range from 470 to 750 nm by simply adjusting the QDs core component or shell layers.					
31472861	2	54	theme	fluorescent	342:352	arg1	aerogels					354:361	novel fluorescent aerogels	336:361	novel fluorescent aerogels	336:361	Here, we fabricated novel fluorescent aerogels with tunable emission by covalently binding environmentally-friendly ZnS(CuInS2)/ZnS core-shell quantum dots along the nanofibrillar BC.					
29763698	5	0	theme	encapsulation	834:846	arg1	efficiency					848:857	68% encapsulation efficiency	830:857	68% encapsulation efficiency	830:857	The zidovudine, an antiretroviral drug was encapsulated in 30 mg of alginate-Cu0.7Zn0.3O with 68% encapsulation efficiency.					
29763698	8	1	theme	devastation	1222:1232	arg1	property					1234:1241	excellent bacterial devastation property	1202:1241	excellent bacterial devastation property	1202:1241	Results herein demonstrated that A-Cu0.7Zn0.3O, A-Cu0.3Zn0.7O and Cu0.5Zn0.5O exhibited excellent bacterial devastation property.					
29763698	2	2	theme	antiretroviral	372:385	arg1	zidovudine					393:402	zidovudine	393:402	zidovudine	393:402	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	2	2	theme	antiretroviral	372:385	arg1	drug					387:390	the antiretroviral drug	368:390	the antiretroviral drug (zidovudine, AZT)	368:408	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	4	3	theme	alginate	636:643	arg1	matrix					645:650	the alginate matrix	632:650	the alginate matrix	632:650	The metal oxide distribution within the alginate matrix was confirmed from the X-ray diffraction and scanning electron microscopy analyses.					
29763698	0	4	theme	sustained	90:98	arg1	release					100:106	sustained release	90:106	sustained release of antiretroviral drug	90:129	Engineered mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug.					
29763698	6	5	theme	AZT	963:965	arg1	release					952:958	a slow and sustained release	931:958	a slow and sustained release of AZT (~96.2%)	931:974	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	6	6	theme	intestinal	892:901	arg1	pH 7.4					910:915	pH 7.4	910:915	pH 7.4	910:915	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	6	6	theme	intestinal	892:901	arg1	fluid					903:907	simulated intestinal fluid	882:907	simulated intestinal fluid (pH 7.4)	882:916	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	9	7	theme	E.	1339:1340	arg1	DH5a					1347:1350	E. coli DH5a	1339:1350	E. coli DH5a	1339:1350	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	0	8	theme	antiretroviral	111:124	arg1	drug					126:129	antiretroviral drug	111:129	antiretroviral drug	111:129	Engineered mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug.					
29763698	9	9	theme	A-Cu0.3Zn0.7O	1289:1301	arg1	sufficient					1307:1316	sufficient	1307:1316	sufficient	1307:1316	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	9	9	theme	A-Cu0.3Zn0.7O	1289:1301	arg1	dose					1246:1249	A dose	1244:1249	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O	1244:1301	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	7	10	theme	release	998:1004	arg1	kinetics					1006:1013	The AZT release kinetics	990:1013	The AZT release kinetics	990:1013	The AZT release kinetics is sufficiently described by the Korsmeyer-Peppas model and follows the Fickian transport profile.					
29763698	5	11	with	30 mg	795:799	arg1	efficiency					848:857	68% encapsulation efficiency	830:857	68% encapsulation efficiency	830:857	The zidovudine, an antiretroviral drug was encapsulated in 30 mg of alginate-Cu0.7Zn0.3O with 68% encapsulation efficiency.					
29763698	4	12	theme	diffraction	681:691	arg1	analyses					726:733	the X-ray diffraction and scanning electron microscopy analyses	671:733	the X-ray diffraction and scanning electron microscopy analyses	671:733	The metal oxide distribution within the alginate matrix was confirmed from the X-ray diffraction and scanning electron microscopy analyses.					
29763698	9	13	theme	A-Cu0.7Zn0.3O	1262:1274	arg1	sufficient					1307:1316	sufficient	1307:1316	sufficient	1307:1316	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	9	13	theme	A-Cu0.7Zn0.3O	1262:1274	arg1	dose					1246:1249	A dose	1244:1249	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O	1244:1301	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	8	14	theme	excellent	1202:1210	arg1	property					1234:1241	excellent bacterial devastation property	1202:1241	excellent bacterial devastation property	1202:1241	Results herein demonstrated that A-Cu0.7Zn0.3O, A-Cu0.3Zn0.7O and Cu0.5Zn0.5O exhibited excellent bacterial devastation property.					
29763698	5	15	theme	alginate-Cu0.7Zn0.3O	804:823	arg1	30 mg					795:799	30 mg	795:799	30 mg of alginate-Cu0.7Zn0.3O with 68% encapsulation efficiency	795:857	The zidovudine, an antiretroviral drug was encapsulated in 30 mg of alginate-Cu0.7Zn0.3O with 68% encapsulation efficiency.					
29763698	6	16	theme	sustained	942:950	arg1	release					952:958	a slow and sustained release	931:958	a slow and sustained release of AZT (~96.2%)	931:974	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	2	17	theme	remarkable	424:433	arg1	properties					449:458	remarkable antibacterial properties	424:458	remarkable antibacterial properties	424:458	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	3	18	theme	Brunauer-Emmett-Teller	563:584	arg1	analysis					586:593	the Brunauer-Emmett-Teller analysis	559:593	the Brunauer-Emmett-Teller analysis	559:593	The resulting polymeric composites had specific surface areas of 185.2-198.6 m2/g as confirmed by the Brunauer-Emmett-Teller analysis.					
29763698	0	19	theme	drug	126:129	arg1	activity					77:84	enhanced antimicrobial activity	54:84	enhanced antimicrobial activity	54:84	Engineered mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug.					
29763698	0	19	theme	drug	126:129	arg1	release					100:106	sustained release	90:106	sustained release of antiretroviral drug	90:129	Engineered mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug.					
29763698	9	20	theme	13 μg/mL	1280:1287	arg1	A-Cu0.3Zn0.7O					1289:1301	13 μg/mL A-Cu0.3Zn0.7O	1280:1301	13 μg/mL A-Cu0.3Zn0.7O	1280:1301	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	9	21	dep	E.	1339:1340	arg1	coli					1342:1345	coli	1342:1345	coli	1342:1345	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	9	22	theme	killed	1332:1337	arg1	NSUHS-151					1366:1374	completely killed E. coli DH5a and S. aureus NSUHS-151	1321:1374	completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h	1321:1386	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	1	23	theme	sodium	205:210	arg1	alginate					212:219	sodium alginate	205:219	sodium alginate	205:219	Here, pH-responsive engineered polymeric composites were fabricated from sodium alginate and mixed Cu/Zn oxides.					
29763698	0	24	theme	mixed	11:15	arg1	composites					39:48	mixed oxide-based polymeric composites	11:48	mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug	11:129	Engineered mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug.					
29763698	6	25	theme	slow	933:936	arg1	release					952:958	a slow and sustained release	931:958	a slow and sustained release of AZT (~96.2%)	931:974	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	4	26	theme	microscopy	715:724	arg1	analyses					726:733	the X-ray diffraction and scanning electron microscopy analyses	671:733	the X-ray diffraction and scanning electron microscopy analyses	671:733	The metal oxide distribution within the alginate matrix was confirmed from the X-ray diffraction and scanning electron microscopy analyses.					
29763698	7	27	theme	Fickian	1087:1093	arg1	profile					1105:1111	the Fickian transport profile	1083:1111	the Fickian transport profile	1083:1111	The AZT release kinetics is sufficiently described by the Korsmeyer-Peppas model and follows the Fickian transport profile.					
29763698	4	28	theme	oxide	606:610	arg1	distribution					612:623	The metal oxide distribution	596:623	The metal oxide distribution within the alginate matrix	596:650	The metal oxide distribution within the alginate matrix was confirmed from the X-ray diffraction and scanning electron microscopy analyses.					
29763698	0	29	theme	polymeric	29:37	arg1	composites					39:48	mixed oxide-based polymeric composites	11:48	mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug	11:129	Engineered mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug.					
29763698	4	30	theme	metal	600:604	arg1	distribution					612:623	The metal oxide distribution	596:623	The metal oxide distribution within the alginate matrix	596:650	The metal oxide distribution within the alginate matrix was confirmed from the X-ray diffraction and scanning electron microscopy analyses.					
29763698	6	31	theme	AZT	875:877	arg1	release					864:870	The release	860:870	The release of AZT in simulated intestinal fluid (pH 7.4)	860:916	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	9	32	theme	8 μg/mL	1254:1260	arg1	A-Cu0.7Zn0.3O					1262:1274	8 μg/mL A-Cu0.7Zn0.3O	1254:1274	8 μg/mL A-Cu0.7Zn0.3O	1254:1274	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	7	33	theme	AZT	994:996	arg1	kinetics					1006:1013	The AZT release kinetics	990:1013	The AZT release kinetics	990:1013	The AZT release kinetics is sufficiently described by the Korsmeyer-Peppas model and follows the Fickian transport profile.					
29763698	2	34	theme	antibacterial	435:447	arg1	properties					449:458	remarkable antibacterial properties	424:458	remarkable antibacterial properties	424:458	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	0	35	theme	oxide-based	17:27	arg1	composites					39:48	mixed oxide-based polymeric composites	11:48	mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug	11:129	Engineered mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug.					
29763698	1	36	theme	Cu/Zn	231:235	arg1	oxides					237:242	mixed Cu/Zn oxides	225:242	mixed Cu/Zn oxides	225:242	Here, pH-responsive engineered polymeric composites were fabricated from sodium alginate and mixed Cu/Zn oxides.					
29763698	7	37	theme	transport	1095:1103	arg1	profile					1105:1111	the Fickian transport profile	1083:1111	the Fickian transport profile	1083:1111	The AZT release kinetics is sufficiently described by the Korsmeyer-Peppas model and follows the Fickian transport profile.					
29763698	4	38	theme	electron	706:713	arg1	microscopy					715:724	scanning electron microscopy	697:724	scanning electron microscopy	697:724	The metal oxide distribution within the alginate matrix was confirmed from the X-ray diffraction and scanning electron microscopy analyses.					
29763698	3	39	theme	polymeric	475:483	arg1	composites					485:494	The resulting polymeric composites	461:494	The resulting polymeric composites	461:494	The resulting polymeric composites had specific surface areas of 185.2-198.6 m2/g as confirmed by the Brunauer-Emmett-Teller analysis.					
29763698	8	40	theme	bacterial	1212:1220	arg1	property					1234:1241	excellent bacterial devastation property	1202:1241	excellent bacterial devastation property	1202:1241	Results herein demonstrated that A-Cu0.7Zn0.3O, A-Cu0.3Zn0.7O and Cu0.5Zn0.5O exhibited excellent bacterial devastation property.					
29763698	7	41	theme	Korsmeyer-Peppas	1048:1063	arg1	model					1065:1069	the Korsmeyer-Peppas model	1044:1069	the Korsmeyer-Peppas model	1044:1069	The AZT release kinetics is sufficiently described by the Korsmeyer-Peppas model and follows the Fickian transport profile.					
29763698	4	42	theme	scanning	697:704	arg1	microscopy					715:724	scanning electron microscopy	697:724	scanning electron microscopy	697:724	The metal oxide distribution within the alginate matrix was confirmed from the X-ray diffraction and scanning electron microscopy analyses.					
29763698	0	43	theme	antimicrobial	63:75	arg1	activity					77:84	enhanced antimicrobial activity	54:84	enhanced antimicrobial activity	54:84	Engineered mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug.					
29763698	3	44	contain	had	496:498	arg1	composites					485:494	The resulting polymeric composites	461:494	The resulting polymeric composites	461:494	The resulting polymeric composites had specific surface areas of 185.2-198.6 m2/g as confirmed by the Brunauer-Emmett-Teller analysis.					
29763698	3	44	contain	had	496:498	arg2	areas					517:521	specific surface areas	500:521	specific surface areas of 185.2-198.6 m2/g	500:541	The resulting polymeric composites had specific surface areas of 185.2-198.6 m2/g as confirmed by the Brunauer-Emmett-Teller analysis.					
29763698	2	45	theme	resulting	249:257	arg1	carrier					356:362	an efficient carrier	343:362	an efficient carrier for the antiretroviral drug (zidovudine, AZT)	343:408	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	2	45	theme	resulting	249:257	arg1	composites					278:287	The resulting alginate-CuxZn1-xO composites	245:287	The resulting alginate-CuxZn1-xO composites	245:287	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	3	46	theme	specific	500:507	arg1	areas					517:521	specific surface areas	500:521	specific surface areas of 185.2-198.6 m2/g	500:541	The resulting polymeric composites had specific surface areas of 185.2-198.6 m2/g as confirmed by the Brunauer-Emmett-Teller analysis.					
29763698	0	47	theme	enhanced	54:61	arg1	activity					77:84	enhanced antimicrobial activity	54:84	enhanced antimicrobial activity	54:84	Engineered mixed oxide-based polymeric composites for enhanced antimicrobial activity and sustained release of antiretroviral drug.					
29763698	3	48	theme	surface	509:515	arg1	areas					517:521	specific surface areas	500:521	specific surface areas of 185.2-198.6 m2/g	500:541	The resulting polymeric composites had specific surface areas of 185.2-198.6 m2/g as confirmed by the Brunauer-Emmett-Teller analysis.					
29763698	3	49	theme	185.2-198.6 m2/g	526:541	arg1	areas					517:521	specific surface areas	500:521	specific surface areas of 185.2-198.6 m2/g	500:541	The resulting polymeric composites had specific surface areas of 185.2-198.6 m2/g as confirmed by the Brunauer-Emmett-Teller analysis.					
29763698	9	50	theme	DH5a	1347:1350	arg1	NSUHS-151					1366:1374	completely killed E. coli DH5a and S. aureus NSUHS-151	1321:1374	completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h	1321:1386	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
29763698	6	51	theme	simulated	882:890	arg1	pH 7.4					910:915	pH 7.4	910:915	pH 7.4	910:915	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	6	51	theme	simulated	882:890	arg1	fluid					903:907	simulated intestinal fluid	882:907	simulated intestinal fluid (pH 7.4)	882:916	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	1	52	theme	pH-responsive	138:150	arg1	composites					173:182	pH-responsive engineered polymeric composites	138:182	pH-responsive engineered polymeric composites	138:182	Here, pH-responsive engineered polymeric composites were fabricated from sodium alginate and mixed Cu/Zn oxides.					
29763698	5	53	theme	antiretroviral	755:768	arg1	zidovudine					740:749	The zidovudine	736:749	The zidovudine	736:749	The zidovudine, an antiretroviral drug was encapsulated in 30 mg of alginate-Cu0.7Zn0.3O with 68% encapsulation efficiency.					
29763698	5	53	theme	antiretroviral	755:768	arg1	drug					770:773	an antiretroviral drug	752:773	an antiretroviral drug	752:773	The zidovudine, an antiretroviral drug was encapsulated in 30 mg of alginate-Cu0.7Zn0.3O with 68% encapsulation efficiency.					
29763698	2	54	dep	zidovudine	393:402	arg1	AZT					405:407	AZT	405:407	AZT	405:407	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	1	55	theme	engineered	152:161	arg1	composites					173:182	pH-responsive engineered polymeric composites	138:182	pH-responsive engineered polymeric composites	138:182	Here, pH-responsive engineered polymeric composites were fabricated from sodium alginate and mixed Cu/Zn oxides.					
29763698	2	56	used	used	335:338	arg2	composites					278:287	The resulting alginate-CuxZn1-xO composites	245:287	The resulting alginate-CuxZn1-xO composites	245:287	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	2	56	used	used	335:338	arg2	carrier					356:362	an efficient carrier	343:362	an efficient carrier for the antiretroviral drug (zidovudine, AZT)	343:408	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	2	57	theme	efficient	346:354	arg1	carrier					356:362	an efficient carrier	343:362	an efficient carrier for the antiretroviral drug (zidovudine, AZT)	343:408	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	2	57	theme	efficient	346:354	arg1	composites					278:287	The resulting alginate-CuxZn1-xO composites	245:287	The resulting alginate-CuxZn1-xO composites	245:287	The resulting alginate-CuxZn1-xO composites were characterized by FTIR, SEM and XRD, then used as an efficient carrier for the antiretroviral drug (zidovudine, AZT) and exhibited remarkable antibacterial properties.					
29763698	5	58	theme	68	830:831	arg1	%					832:832	%	832:832	%	832:832	The zidovudine, an antiretroviral drug was encapsulated in 30 mg of alginate-Cu0.7Zn0.3O with 68% encapsulation efficiency.					
29763698	6	59	from	release	864:870	arg1	pH 7.4					910:915	pH 7.4	910:915	pH 7.4	910:915	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	6	59	from	release	864:870	arg1	fluid					903:907	simulated intestinal fluid	882:907	simulated intestinal fluid (pH 7.4)	882:916	The release of AZT in simulated intestinal fluid (pH 7.4) was studied, a slow and sustained release of AZT (~96.2%) was observed.					
29763698	1	60	theme	mixed	225:229	arg1	oxides					237:242	mixed Cu/Zn oxides	225:242	mixed Cu/Zn oxides	225:242	Here, pH-responsive engineered polymeric composites were fabricated from sodium alginate and mixed Cu/Zn oxides.					
29763698	1	61	theme	polymeric	163:171	arg1	composites					173:182	pH-responsive engineered polymeric composites	138:182	pH-responsive engineered polymeric composites	138:182	Here, pH-responsive engineered polymeric composites were fabricated from sodium alginate and mixed Cu/Zn oxides.					
29763698	3	62	theme	resulting	465:473	arg1	composites					485:494	The resulting polymeric composites	461:494	The resulting polymeric composites	461:494	The resulting polymeric composites had specific surface areas of 185.2-198.6 m2/g as confirmed by the Brunauer-Emmett-Teller analysis.					
29763698	4	63	theme	X-ray	675:679	arg1	diffraction					681:691	X-ray diffraction	675:691	X-ray diffraction	675:691	The metal oxide distribution within the alginate matrix was confirmed from the X-ray diffraction and scanning electron microscopy analyses.					
29763698	5	64	theme	%	832:832	arg1	efficiency					848:857	68% encapsulation efficiency	830:857	68% encapsulation efficiency	830:857	The zidovudine, an antiretroviral drug was encapsulated in 30 mg of alginate-Cu0.7Zn0.3O with 68% encapsulation efficiency.					
29763698	9	65	theme	aureus	1359:1364	arg1	NSUHS-151					1366:1374	completely killed E. coli DH5a and S. aureus NSUHS-151	1321:1374	completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h	1321:1386	A dose of 8 μg/mL A-Cu0.7Zn0.3O and 13 μg/mL A-Cu0.3Zn0.7O are sufficient to completely killed E. coli DH5a and S. aureus NSUHS-151 within 24 h.					
31502406	7	0	theme	Sterilized	1177:1186	arg1	hydrogels					1188:1196	Sterilized hydrogels	1177:1196	Sterilized hydrogels by steam sterilization	1177:1219	Sterilized hydrogels by steam sterilization can be good candidates as scaffolds for tissue engineering applications.					
31502406	2	1	theme	thermal	416:422	arg1	analysis					424:431	simultaneous thermal analysis	403:431	simultaneous thermal analysis	403:431	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	5	2	from	rad/s	1051:1055	arg1	frequency					1062:1070	low frequency	1058:1070	low frequency	1058:1070	Also, hydrogels exhibiting a solid-like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency).					
31502406	5	2	from	rad/s	1051:1055	arg1	Pa					1041:1042	48,705 ± 2,453 Pa	1026:1042	48,705 ± 2,453 Pa at 0.1 rad/s (low frequency)	1026:1071	Also, hydrogels exhibiting a solid-like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency).					
31502406	2	3	theme	simultaneous	403:414	arg1	analysis					424:431	simultaneous thermal analysis	403:431	simultaneous thermal analysis	403:431	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	4	theme	tetrazolium	607:617	arg1	assay					642:646	3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay	565:646	assay	642:646	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	4	theme	tetrazolium	607:617	arg1	assay					653:657	MTT assay	649:657	MTT assay	649:657	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	3	5	theme	decomposition	696:708	arg1	temperature					716:726	a decomposition onset temperature	694:726	a decomposition onset temperature	694:726	The samples showed a decomposition onset temperature below 253.3 ± 4.8°C, a semicrystalline nature, and a highly porous structure.					
31502406	4	6	theme	maximum	828:834	arg1	uptake					842:847	the maximum water uptake	824:847	the maximum water uptake in phosphate-buffered saline	824:876	Hydrogels reached the maximum water uptake in phosphate-buffered saline after 80 min, showing values from nine to twelve times their dry mass.					
31502406	2	7	theme	-2,5-diphenyl	593:605	arg1	assay					642:646	3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay	565:646	assay	642:646	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	7	theme	-2,5-diphenyl	593:605	arg1	assay					653:657	MTT assay	649:657	MTT assay	649:657	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	8	theme	mechanical	328:337	arg1	properties					339:348	mechanical properties	328:348	mechanical properties	328:348	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	9	theme	hydrogels	375:383	arg1	stability					271:279	The thermal stability	259:279	The thermal stability	259:279	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	9	theme	hydrogels	375:383	arg1	biocompatibility					355:370	biocompatibility	355:370	biocompatibility	355:370	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	9	theme	hydrogels	375:383	arg1	area					322:325	surface area	314:325	surface area	314:325	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	9	theme	hydrogels	375:383	arg1	structure					291:299	chemical structure	282:299	chemical structure	282:299	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	9	theme	hydrogels	375:383	arg1	properties					339:348	mechanical properties	328:348	mechanical properties	328:348	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	9	theme	hydrogels	375:383	arg1	morphology					302:311	morphology	302:311	morphology	302:311	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	5	10	theme	low	1058:1060	arg1	frequency					1062:1070	low frequency	1058:1070	low frequency	1058:1070	Also, hydrogels exhibiting a solid-like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency).					
31502406	5	10	theme	low	1058:1060	arg1	Pa					1041:1042	48,705 ± 2,453 Pa	1026:1042	48,705 ± 2,453 Pa at 0.1 rad/s (low frequency)	1026:1071	Also, hydrogels exhibiting a solid-like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency).					
31502406	7	11	theme	good	1228:1231	arg1	candidates					1233:1242	good candidates	1228:1242	good candidates	1228:1242	Sterilized hydrogels by steam sterilization can be good candidates as scaffolds for tissue engineering applications.					
31502406	4	12	from	uptake	842:847	arg1	saline					871:876	phosphate-buffered saline	852:876	phosphate-buffered saline	852:876	Hydrogels reached the maximum water uptake in phosphate-buffered saline after 80 min, showing values from nine to twelve times their dry mass.					
31502406	2	13	theme	[5-dimethylthiazol-2-yl	569:591	arg1	assay					642:646	3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay	565:646	assay	642:646	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	13	theme	[5-dimethylthiazol-2-yl	569:591	arg1	assay					653:657	MTT assay	649:657	MTT assay	649:657	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	3	14	theme	porous	788:793	arg1	structure					795:803	a highly porous structure	779:803	a highly porous structure	779:803	The samples showed a decomposition onset temperature below 253.3 ± 4.8°C, a semicrystalline nature, and a highly porous structure.					
31502406	4	15	theme	phosphate-buffered	852:869	arg1	saline					871:876	phosphate-buffered saline	852:876	phosphate-buffered saline	852:876	Hydrogels reached the maximum water uptake in phosphate-buffered saline after 80 min, showing values from nine to twelve times their dry mass.					
31502406	2	16	theme	infrared	452:459	arg1	spectroscopy					461:472	infrared spectroscopy	452:472	infrared spectroscopy	452:472	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	0	17	theme	chitosan-based	11:24	arg1	cytotoxicity					93:104	in vitro cytotoxicity	84:104	in vitro cytotoxicity	84:104	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	17	theme	chitosan-based	11:24	arg1	hydrogels					36:44	Sterilized chitosan-based composite hydrogels	0:44	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.	0:105	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	17	theme	chitosan-based	11:24	arg1	characterization					63:78	Physicochemical characterization	47:78	Physicochemical characterization	47:78	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	5	18	theme	solid-like	978:987	arg1	behavior					989:996	a solid-like behavior	976:996	a solid-like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency)	976:1071	Also, hydrogels exhibiting a solid-like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency).					
31502406	4	19	theme	dry	939:941	arg1	mass					943:946	their dry mass	933:946	nine to twelve times their dry mass	912:946	Hydrogels reached the maximum water uptake in phosphate-buffered saline after 80 min, showing values from nine to twelve times their dry mass.					
31502406	4	20	dep	twelve	920:925	arg1	to					917:918	to	917:918	to	917:918	Hydrogels reached the maximum water uptake in phosphate-buffered saline after 80 min, showing values from nine to twelve times their dry mass.					
31502406	1	21	theme	Gelatin/chitosan/polyvinyl	107:132	arg1	hydrogels					142:150	Gelatin/chitosan/polyvinyl alcohol hydrogels	107:150	Gelatin/chitosan/polyvinyl alcohol hydrogels	107:150	Gelatin/chitosan/polyvinyl alcohol hydrogels were fabricated at different polymer ratios using the freeze-drying and sterilized by steam sterilization.					
31502406	0	22	theme	Sterilized	0:9	arg1	cytotoxicity					93:104	in vitro cytotoxicity	84:104	in vitro cytotoxicity	84:104	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	22	theme	Sterilized	0:9	arg1	hydrogels					36:44	Sterilized chitosan-based composite hydrogels	0:44	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.	0:105	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	22	theme	Sterilized	0:9	arg1	characterization					63:78	Physicochemical characterization	47:78	Physicochemical characterization	47:78	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	1	23	dep	freeze-drying	206:218	arg1	the					202:204	the	202:204	the	202:204	Gelatin/chitosan/polyvinyl alcohol hydrogels were fabricated at different polymer ratios using the freeze-drying and sterilized by steam sterilization.					
31502406	1	24	theme	steam	238:242	arg1	sterilization					244:256	steam sterilization	238:256	steam sterilization	238:256	Gelatin/chitosan/polyvinyl alcohol hydrogels were fabricated at different polymer ratios using the freeze-drying and sterilized by steam sterilization.					
31502406	4	25	dep	times	927:931	arg1	mass					943:946	their dry mass	933:946	nine to twelve times their dry mass	912:946	Hydrogels reached the maximum water uptake in phosphate-buffered saline after 80 min, showing values from nine to twelve times their dry mass.					
31502406	4	26	theme	water	836:840	arg1	uptake					842:847	the maximum water uptake	824:847	the maximum water uptake in phosphate-buffered saline	824:876	Hydrogels reached the maximum water uptake in phosphate-buffered saline after 80 min, showing values from nine to twelve times their dry mass.					
31502406	1	27	theme	alcohol	134:140	arg1	hydrogels					142:150	Gelatin/chitosan/polyvinyl alcohol hydrogels	107:150	Gelatin/chitosan/polyvinyl alcohol hydrogels	107:150	Gelatin/chitosan/polyvinyl alcohol hydrogels were fabricated at different polymer ratios using the freeze-drying and sterilized by steam sterilization.					
31502406	2	28	theme	viability	632:640	arg1	assay					642:646	3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay	565:646	assay	642:646	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	28	theme	viability	632:640	arg1	assay					653:657	MTT assay	649:657	MTT assay	649:657	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	0	29	dep	in	84:85	arg1	vitro					87:91	vitro	87:91	vitro	87:91	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	30	theme	Physicochemical	47:61	arg1	hydrogels					36:44	Sterilized chitosan-based composite hydrogels	0:44	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.	0:105	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	30	theme	Physicochemical	47:61	arg1	characterization					63:78	Physicochemical characterization	47:78	Physicochemical characterization	47:78	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	2	31	theme	cell	627:630	arg1	assay					642:646	3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay	565:646	assay	642:646	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	31	theme	cell	627:630	arg1	assay					653:657	MTT assay	649:657	MTT assay	649:657	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	6	32	theme	low	1106:1108	arg1	cytotoxicity					1110:1121	low cytotoxicity	1106:1121	low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line	1106:1174	The sterilized hydrogels showed low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line.					
31502406	6	32	theme	low	1106:1108	arg1	%					1143:1143	cell viability > 70%	1124:1143	cell viability > 70%	1124:1143	The sterilized hydrogels showed low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line.					
31502406	6	33	theme	cell	1124:1127	arg1	cytotoxicity					1110:1121	low cytotoxicity	1106:1121	low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line	1106:1174	The sterilized hydrogels showed low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line.					
31502406	6	33	theme	cell	1124:1127	arg1	%					1143:1143	cell viability > 70%	1124:1143	cell viability > 70%	1124:1143	The sterilized hydrogels showed low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line.					
31502406	2	34	theme	X-ray	475:479	arg1	diffraction					481:491	X-ray diffraction	475:491	X-ray diffraction	475:491	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	35	theme	bromide	619:625	arg1	assay					642:646	3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay	565:646	assay	642:646	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	35	theme	bromide	619:625	arg1	assay					653:657	MTT assay	649:657	MTT assay	649:657	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	3	36	theme	onset	710:714	arg1	temperature					716:726	a decomposition onset temperature	694:726	a decomposition onset temperature	694:726	The samples showed a decomposition onset temperature below 253.3 ± 4.8°C, a semicrystalline nature, and a highly porous structure.					
31502406	2	37	theme	chemical	282:289	arg1	structure					291:299	chemical structure	282:299	chemical structure	282:299	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	38	theme	nitrogen	540:547	arg1	microscopy					503:512	confocal microscopy	494:512	confocal microscopy	494:512	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	38	theme	nitrogen	540:547	arg1	assay					653:657	MTT assay	649:657	MTT assay	649:657	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	38	theme	nitrogen	540:547	arg1	adsorption/desorption					515:535	adsorption/desorption	515:535	adsorption/desorption of nitrogen	515:547	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	38	theme	nitrogen	540:547	arg1	analysis					424:431	simultaneous thermal analysis	403:431	simultaneous thermal analysis	403:431	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	38	theme	nitrogen	540:547	arg1	diffraction					481:491	X-ray diffraction	475:491	X-ray diffraction	475:491	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	38	theme	nitrogen	540:547	arg1	Fourier					434:440	Fourier	434:440	Fourier transform infrared spectroscopy	434:472	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	38	theme	nitrogen	540:547	arg1	assay					642:646	3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay	565:646	assay	642:646	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	38	theme	nitrogen	540:547	arg1	rheometry					550:558	rheometry	550:558	rheometry	550:558	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	7	39	theme	tissue	1261:1266	arg1	applications					1280:1291	tissue engineering applications	1261:1291	tissue engineering applications	1261:1291	Sterilized hydrogels by steam sterilization can be good candidates as scaffolds for tissue engineering applications.					
31502406	2	40	theme	3-4	565:567	arg1	assay					642:646	3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay	565:646	assay	642:646	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	40	theme	3-4	565:567	arg1	assay					653:657	MTT assay	649:657	MTT assay	649:657	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	4	41	from	times	927:931	arg1	values					900:905	values	900:905	values from nine to twelve times their dry mass	900:946	Hydrogels reached the maximum water uptake in phosphate-buffered saline after 80 min, showing values from nine to twelve times their dry mass.					
31502406	7	42	theme	steam	1201:1205	arg1	sterilization					1207:1219	steam sterilization	1201:1219	steam sterilization	1201:1219	Sterilized hydrogels by steam sterilization can be good candidates as scaffolds for tissue engineering applications.					
31502406	2	43	theme	thermal	263:269	arg1	stability					271:279	The thermal stability	259:279	The thermal stability	259:279	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	44	theme	MTT	649:651	arg1	assay					642:646	3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay	565:646	assay	642:646	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	44	theme	MTT	649:651	arg1	assay					653:657	MTT assay	649:657	MTT assay	649:657	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	2	45	theme	surface	314:320	arg1	area					322:325	surface area	314:325	surface area	314:325	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	0	46	dep	hydrogels	36:44	arg1	cytotoxicity					93:104	in vitro cytotoxicity	84:104	in vitro cytotoxicity	84:104	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	46	dep	hydrogels	36:44	arg1	hydrogels					36:44	Sterilized chitosan-based composite hydrogels	0:44	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.	0:105	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	46	dep	hydrogels	36:44	arg1	characterization					63:78	Physicochemical characterization	47:78	Physicochemical characterization	47:78	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	1	47	theme	different	171:179	arg1	ratios					189:194	different polymer ratios	171:194	different polymer ratios using the freeze-drying and sterilized by steam sterilization	171:256	Gelatin/chitosan/polyvinyl alcohol hydrogels were fabricated at different polymer ratios using the freeze-drying and sterilized by steam sterilization.					
31502406	5	48	theme	48,705	1026:1031	arg1	frequency					1062:1070	low frequency	1058:1070	low frequency	1058:1070	Also, hydrogels exhibiting a solid-like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency).					
31502406	5	48	theme	48,705	1026:1031	arg1	Pa					1041:1042	48,705 ± 2,453 Pa	1026:1042	48,705 ± 2,453 Pa at 0.1 rad/s (low frequency)	1026:1071	Also, hydrogels exhibiting a solid-like behavior ranging from 2,567 ± 467 to 48,705 ± 2,453 Pa at 0.1 rad/s (low frequency).					
31502406	2	49	dep	Fourier	434:440	arg1	transform					442:450	transform	442:450	transform infrared spectroscopy	442:472	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	1	50	theme	polymer	181:187	arg1	ratios					189:194	different polymer ratios	171:194	different polymer ratios using the freeze-drying and sterilized by steam sterilization	171:256	Gelatin/chitosan/polyvinyl alcohol hydrogels were fabricated at different polymer ratios using the freeze-drying and sterilized by steam sterilization.					
31502406	6	51	theme	sterilized	1078:1087	arg1	hydrogels					1089:1097	The sterilized hydrogels	1074:1097	The sterilized hydrogels	1074:1097	The sterilized hydrogels showed low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line.					
31502406	3	52	theme	a	749:749	arg1	nature					767:772	a semicrystalline nature	749:772	a semicrystalline nature	749:772	The samples showed a decomposition onset temperature below 253.3 ± 4.8°C, a semicrystalline nature, and a highly porous structure.					
31502406	2	53	theme	confocal	494:501	arg1	microscopy					503:512	confocal microscopy	494:512	confocal microscopy	494:512	The thermal stability, chemical structure, morphology, surface area, mechanical properties, and biocompatibility of hydrogels were evaluated by simultaneous thermal analysis, Fourier transform infrared spectroscopy, X-ray diffraction, confocal microscopy, adsorption/desorption of nitrogen, rheometry, and 3-4,[5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide cell viability assay (MTT assay), respectively.					
31502406	6	54	theme	cell	1166:1169	arg1	line					1171:1174	the HT29-MTX-E12 cell line	1149:1174	the HT29-MTX-E12 cell line	1149:1174	The sterilized hydrogels showed low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line.					
31502406	3	55	theme	semicrystalline	751:765	arg1	nature					767:772	a semicrystalline nature	749:772	a semicrystalline nature	749:772	The samples showed a decomposition onset temperature below 253.3 ± 4.8°C, a semicrystalline nature, and a highly porous structure.					
31502406	0	56	theme	in	84:85	arg1	cytotoxicity					93:104	in vitro cytotoxicity	84:104	in vitro cytotoxicity	84:104	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	56	theme	in	84:85	arg1	hydrogels					36:44	Sterilized chitosan-based composite hydrogels	0:44	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.	0:105	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	57	theme	composite	26:34	arg1	cytotoxicity					93:104	in vitro cytotoxicity	84:104	in vitro cytotoxicity	84:104	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	57	theme	composite	26:34	arg1	hydrogels					36:44	Sterilized chitosan-based composite hydrogels	0:44	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.	0:105	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	0	57	theme	composite	26:34	arg1	characterization					63:78	Physicochemical characterization	47:78	Physicochemical characterization	47:78	Sterilized chitosan-based composite hydrogels: Physicochemical characterization and in vitro cytotoxicity.					
31502406	6	58	theme	HT29-MTX-E12	1153:1164	arg1	line					1171:1174	the HT29-MTX-E12 cell line	1149:1174	the HT29-MTX-E12 cell line	1149:1174	The sterilized hydrogels showed low cytotoxicity (cell viability > 70%) to the HT29-MTX-E12 cell line.					
31502406	7	59	theme	engineering	1268:1278	arg1	applications					1280:1291	tissue engineering applications	1261:1291	tissue engineering applications	1261:1291	Sterilized hydrogels by steam sterilization can be good candidates as scaffolds for tissue engineering applications.					
30851844	3	0	gly	glycopeptides	602:614	arg2	glycopeptides					602:614	lowly abundant glycopeptides	587:614	lowly abundant glycopeptides	587:614	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	0	1	theme	exclusion	75:83	arg1	chromatography					85:98	size exclusion chromatography	70:98	size exclusion chromatography	70:98	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.					
30851844	4	2	theme	chromatography	784:797	arg1	effects					829:835	the size exclusion chromatography and hydrophilic (named SELIC) effects	765:835	the size exclusion chromatography and hydrophilic (named SELIC) effects	765:835	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	5	3	theme	paraffin-embedded	1126:1142	arg1	tissues					1151:1157	formalin-fixed and paraffin-embedded (FFPE) tissues	1107:1157	formalin-fixed and paraffin-embedded (FFPE) tissues	1107:1157	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	2	4	theme	associated	324:333	arg1	heterogeneity					335:347	its associated heterogeneity	320:347	its associated heterogeneity	320:347	Specially, site-specific glycosylation and its associated heterogeneity are closely related to the function and activity of the glycoprotein.					
30851844	3	5	gly	non-glycosylated	528:543	arg1	peptides					545:552	highly abundant non-glycosylated peptides	512:552	highly abundant non-glycosylated peptides	512:552	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	4	6	theme	practical	654:662	arg1	method					705:710	a practical intact tryptic N-glycopeptide enrichment method	652:710	a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides	652:958	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	3	7	gly	N-glycopeptide	435:448	arg2	N-glycopeptide					435:448	intact N-glycopeptide analysis	428:457	intact N-glycopeptide analysis	428:457	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	4	8	gly	N-glycopeptide	679:692	arg2	N-glycopeptide					679:692	a practical intact tryptic N-glycopeptide enrichment method	652:710	a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides	652:958	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	5	9	theme	N-glycopeptides	1008:1022	arg1	analysis					1024:1031	the intact N-glycopeptides analysis	997:1031	the intact N-glycopeptides analysis of complex protein mixtures	997:1059	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	4	10	gly	N-glycopeptides	918:932	arg2	N-glycopeptides					918:932	enrich intact N-glycopeptides	904:932	the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides	765:932	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	3	11	theme	abundant	593:600	arg1	glycopeptides					602:614	lowly abundant glycopeptides	587:614	lowly abundant glycopeptides	587:614	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	5	12	theme	intact	1001:1006	arg1	analysis					1024:1031	the intact N-glycopeptides analysis	997:1031	the intact N-glycopeptides analysis of complex protein mixtures	997:1059	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	1	13	theme	Increasing	177:186	arg1	researches					188:197	Increasing researches	177:197	Increasing researches	177:197	Increasing researches proved that abnormal glycosylation is strongly correlated with many diseases.					
30851844	6	14	gly	glycosylation	1283:1295	arg2	sites					1297:1301	261 glycosylation sites	1279:1301	261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues	1279:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	6	14	gly	glycosylation	1283:1295	arg2	261					1279:1281	261	1279:1281	261	1279:1281	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	4	15	theme	intact	911:916	arg1	N-glycopeptides					918:932	enrich intact N-glycopeptides	904:932	the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides	765:932	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	3	16	theme	glycopeptides	602:614	arg1	ionization					573:582	the ionization	569:582	the ionization of lowly abundant glycopeptides	569:614	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	0	17	theme	practical	2:10	arg1	approach					12:19	A practical approach	0:19	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC)	0:125	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.					
30851844	6	18	gly	glycoproteins	1310:1322	arg1	glycoproteins					1310:1322	191 glycoproteins	1306:1322	191 glycoproteins from FFPE thymoma tissues	1306:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	6	19	from	sites	1297:1301	arg1	glycoproteins					1310:1322	191 glycoproteins	1306:1322	191 glycoproteins from FFPE thymoma tissues	1306:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	3	20	theme	great	471:475	arg1	challenges					477:486	great challenges	471:486	great challenges	471:486	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	3	21	theme	intact	428:433	arg1	analysis					450:457	intact N-glycopeptide analysis	428:457	intact N-glycopeptide analysis	428:457	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	4	22	theme	present	624:630	arg1	study					632:636	the present study	620:636	the present study	620:636	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	5	23	dep	paraffin-embedded	1126:1142	arg1	FFPE					1145:1148	FFPE	1145:1148	FFPE	1145:1148	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	4	24	dep	hydrophilic	803:813	arg1	SELIC					822:826	SELIC	822:826	SELIC	822:826	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	5	25	gly	N-glycopeptides	1008:1022	arg2	N-glycopeptides					1008:1022	the intact N-glycopeptides analysis	997:1031	the intact N-glycopeptides analysis of complex protein mixtures	997:1059	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	5	26	theme	complex	1036:1042	arg1	mixtures					1052:1059	complex protein mixtures	1036:1059	complex protein mixtures	1036:1059	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	5	27	theme	protein	1044:1050	arg1	mixtures					1052:1059	complex protein mixtures	1036:1059	complex protein mixtures	1036:1059	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	1	28	theme	abnormal	211:218	arg1	glycosylation					220:232	abnormal glycosylation	211:232	abnormal glycosylation	211:232	Increasing researches proved that abnormal glycosylation is strongly correlated with many diseases.					
30851844	6	29	gly	N-glycopeptides	1246:1260	arg2	N-glycopeptides					1246:1260	700 site-specific intact tryptic N-glycopeptides	1213:1260	700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues	1213:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	2	30	gly	glycoprotein	405:416	arg1	glycoprotein					405:416	the glycoprotein	401:416	the glycoprotein	401:416	Specially, site-specific glycosylation and its associated heterogeneity are closely related to the function and activity of the glycoprotein.					
30851844	2	31	dep	function	376:383	arg1	the					372:374	the	372:374	the	372:374	Specially, site-specific glycosylation and its associated heterogeneity are closely related to the function and activity of the glycoprotein.					
30851844	3	32	theme	abundant	519:526	arg1	peptides					545:552	highly abundant non-glycosylated peptides	512:552	highly abundant non-glycosylated peptides	512:552	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	0	33	theme	intact	31:36	arg1	N-glycopeptides					46:60	intact tryptic N-glycopeptides	31:60	intact tryptic N-glycopeptides	31:60	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.					
30851844	6	34	theme	site-specific	1217:1229	arg1	N-glycopeptides					1246:1260	700 site-specific intact tryptic N-glycopeptides	1213:1260	700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues	1213:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	0	35	theme	acrylamide-agarose	136:153	arg1	system					169:174	an acrylamide-agarose composite gel system	133:174	an acrylamide-agarose composite gel system	133:174	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.					
30851844	5	36	theme	formalin-fixed	1107:1120	arg1	tissues					1151:1157	formalin-fixed and paraffin-embedded (FFPE) tissues	1107:1157	formalin-fixed and paraffin-embedded (FFPE) tissues	1107:1157	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	4	37	theme	composite	737:745	arg1	gel					747:749	acrylamide-agarose composite gel	718:749	acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides	718:958	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	4	38	theme	exclusion	774:782	arg1	chromatography					784:797	the size exclusion chromatography	765:797	the size exclusion chromatography	765:797	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	4	39	theme	enrichment	694:703	arg1	method					705:710	a practical intact tryptic N-glycopeptide enrichment method	652:710	a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides	652:958	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	4	40	theme	acrylamide-agarose	718:735	arg1	gel					747:749	acrylamide-agarose composite gel	718:749	acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides	718:958	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	3	41	theme	N-glycopeptide	435:448	arg1	analysis					450:457	intact N-glycopeptide analysis	428:457	intact N-glycopeptide analysis	428:457	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	0	42	theme	gel	165:167	arg1	system					169:174	an acrylamide-agarose composite gel system	133:174	an acrylamide-agarose composite gel system	133:174	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.					
30851844	4	43	theme	enrich	904:909	arg1	N-glycopeptides					918:932	enrich intact N-glycopeptides	904:932	the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides	765:932	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	6	44	theme	intact	1231:1236	arg1	N-glycopeptides					1246:1260	700 site-specific intact tryptic N-glycopeptides	1213:1260	700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues	1213:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	0	45	theme	tryptic	38:44	arg1	N-glycopeptides					46:60	intact tryptic N-glycopeptides	31:60	intact tryptic N-glycopeptides	31:60	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.					
30851844	0	46	theme	composite	155:163	arg1	system					169:174	an acrylamide-agarose composite gel system	133:174	an acrylamide-agarose composite gel system	133:174	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.					
30851844	6	47	theme	thymoma	1334:1340	arg1	tissues					1342:1348	FFPE thymoma tissues	1329:1348	FFPE thymoma tissues	1329:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	4	48	theme	hydrophilic	803:813	arg1	effects					829:835	the size exclusion chromatography and hydrophilic (named SELIC) effects	765:835	the size exclusion chromatography and hydrophilic (named SELIC) effects	765:835	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	6	49	from	tissues	1342:1348	arg1	glycoproteins					1310:1322	191 glycoproteins	1306:1322	191 glycoproteins from FFPE thymoma tissues	1306:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	6	50	theme	FFPE	1329:1332	arg1	tissues					1342:1348	FFPE thymoma tissues	1329:1348	FFPE thymoma tissues	1329:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	2	51	theme	site-specific	288:300	arg1	glycosylation					302:314	site-specific glycosylation	288:314	site-specific glycosylation	288:314	Specially, site-specific glycosylation and its associated heterogeneity are closely related to the function and activity of the glycoprotein.					
30851844	4	52	theme	size	769:772	arg1	chromatography					784:797	the size exclusion chromatography	765:797	the size exclusion chromatography	765:797	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	3	53	theme	non-glycosylated	528:543	arg1	peptides					545:552	highly abundant non-glycosylated peptides	512:552	highly abundant non-glycosylated peptides	512:552	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	4	54	theme	tryptic	671:677	arg1	method					705:710	a practical intact tryptic N-glycopeptide enrichment method	652:710	a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides	652:958	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	5	55	theme	mixtures	1052:1059	arg1	analysis					1024:1031	the intact N-glycopeptides analysis	997:1031	the intact N-glycopeptides analysis of complex protein mixtures	997:1059	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	3	56	theme	peptides	545:552	arg1	presence					500:507	the presence	496:507	the presence of highly abundant non-glycosylated peptides	496:552	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	4	57	theme	intact	664:669	arg1	method					705:710	a practical intact tryptic N-glycopeptide enrichment method	652:710	a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides	652:958	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
30851844	0	58	theme	size	70:73	arg1	chromatography					85:98	size exclusion chromatography	70:98	size exclusion chromatography	70:98	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.					
30851844	5	59	theme	useful	971:976	arg1	tool					978:981	a useful tool	969:981	a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS	969:1164	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	5	59	theme	useful	971:976	arg1	This					961:964	This	961:964	This	961:964	This is a useful tool to facilitate the intact N-glycopeptides analysis of complex protein mixtures, particularly for samples that extracted from formalin-fixed and paraffin-embedded (FFPE) tissues by SDS.					
30851844	2	60	theme	glycoprotein	405:416	arg1	function					376:383	function	376:383	function	376:383	Specially, site-specific glycosylation and its associated heterogeneity are closely related to the function and activity of the glycoprotein.					
30851844	2	60	theme	glycoprotein	405:416	arg1	activity					389:396	activity	389:396	activity	389:396	Specially, site-specific glycosylation and its associated heterogeneity are closely related to the function and activity of the glycoprotein.					
30851844	6	61	theme	glycosylation	1283:1295	arg1	sites					1297:1301	261 glycosylation sites	1279:1301	261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues	1279:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	1	62	theme	many	262:265	arg1	diseases					267:274	many diseases	262:274	many diseases	262:274	Increasing researches proved that abnormal glycosylation is strongly correlated with many diseases.					
30851844	6	63	theme	tryptic	1238:1244	arg1	N-glycopeptides					1246:1260	700 site-specific intact tryptic N-glycopeptides	1213:1260	700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues	1213:1348	Using this method, we successfully identified 700 site-specific intact tryptic N-glycopeptides corresponding to 261 glycosylation sites on 191 glycoproteins from FFPE thymoma tissues.					
30851844	0	64	gly	N-glycopeptides	46:60	arg2	N-glycopeptides					46:60	intact tryptic N-glycopeptides	31:60	intact tryptic N-glycopeptides	31:60	A practical approach to enrich intact tryptic N-glycopeptides through size exclusion chromatography and hydrophilicity (SELIC) using an acrylamide-agarose composite gel system.					
30851844	3	65	theme	lowly	587:591	arg1	glycopeptides					602:614	lowly abundant glycopeptides	587:614	lowly abundant glycopeptides	587:614	However, intact N-glycopeptide analysis still faces great challenges because the presence of highly abundant non-glycosylated peptides would suppress the ionization of lowly abundant glycopeptides.					
30851844	4	66	theme	N-glycopeptide	679:692	arg1	method					705:710	a practical intact tryptic N-glycopeptide enrichment method	652:710	a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides	652:958	In the present study, we developed a practical intact tryptic N-glycopeptide enrichment method using acrylamide-agarose composite gel that combined the size exclusion chromatography and hydrophilic (named SELIC) effects, aimed to remove the detergent rapidly and effectively, as well as enrich intact N-glycopeptides while extracting peptides.					
31639624	6	0	theme	glucan	1319:1324	arg1	genes					1344:1348	glucan synthase-encoding genes	1319:1348	glucan synthase-encoding genes	1319:1348	The ability of PacC to bind to the promoters of glucan synthase-encoding genes confirms that PacC transcriptionally regulates these genes.					
31639624	5	1	theme	PacC-silenced	1081:1093	arg1	strains					1095:1101	the PacC-silenced strains	1077:1101	the PacC-silenced strains	1077:1101	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	4	2	theme	structure	853:861	arg1	images					829:834	transmission electron microscopy images	796:834	transmission electron microscopy images of the cell wall structure	796:861	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	1	3	theme	environmental	159:171	arg1	adaptation					176:185	environmental pH adaptation	159:185	environmental pH adaptation	159:185	The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear.					
31639624	5	4	theme	WT	1148:1149	arg1	strain					1151:1156	the WT strain	1144:1156	the WT strain	1144:1156	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	0	5	theme	wall	68:71	arg1	components					73:82	cell wall components	63:82	cell wall components	63:82	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	3	6	theme	cell	697:700	arg1	stress					707:712	cell wall stress	697:712	cell wall stress	697:712	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	4	7	theme	wall	848:851	arg1	structure					853:861	the cell wall structure	839:861	the cell wall structure	839:861	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	4	8	dep	thinner	924:930	arg1	%					955:955	approximately 25-30%	936:955	approximately 25-30%	936:955	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	1	9	theme	pH	173:174	arg1	adaptation					176:185	environmental pH adaptation	159:185	environmental pH adaptation	159:185	The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear.					
31639624	5	10	theme	wall	1020:1023	arg1	composition					1025:1035	cell wall composition	1015:1035	cell wall composition	1015:1035	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	0	11	theme	Ganoderma	87:95	arg1	lucidum					97:103	Ganoderma lucidum	87:103	Ganoderma lucidum	87:103	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	0	12	from	Influence	0:8	arg1	components					73:82	cell wall components	63:82	cell wall components	63:82	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	0	12	from	Influence	0:8	arg1	adaptability					46:57	environmental stress adaptability	25:57	environmental stress adaptability	25:57	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	4	13	theme	microscopy	818:827	arg1	images					829:834	transmission electron microscopy images	796:834	transmission electron microscopy images of the cell wall structure	796:861	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	1	14	dep	factor	124:129	arg1	PacC/Rim101					131:141	PacC/Rim101	131:141	The transcription factor PacC/Rim101	106:141	The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear.					
31639624	3	15	theme	G.	557:558	arg1	lucidum					560:566	G. lucidum	557:566	G. lucidum	557:566	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	2	16	theme	homologous	363:372	arg1	gene					374:377	a homologous gene	361:377	a homologous gene of PacC/Rim101	361:392	In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed.					
31639624	1	17	theme	fungal	279:284	arg1	adaptation					286:295	fungal adaptation	279:295	fungal adaptation to environmental stress	279:319	The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear.					
31639624	5	18	theme	β-1,3-glucan	1053:1064	arg1	content					1066:1072	the β-1,3-glucan content	1049:1072	the β-1,3-glucan content in the PacC-silenced strains	1049:1101	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	18	theme	β-1,3-glucan	1053:1064	arg1	that					1136:1139	that	1136:1139	that	1136:1139	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	18	theme	β-1,3-glucan	1053:1064	arg1	%					1131:1131	only approximately 78-80%	1107:1131	only approximately 78-80% of that in the WT strain	1107:1156	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	3	19	from	functions	536:544	arg1	lucidum					560:566	G. lucidum	557:566	G. lucidum	557:566	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	0	20	theme	lucidum	97:103	arg1	components					73:82	cell wall components	63:82	cell wall components	63:82	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	0	20	theme	lucidum	97:103	arg1	adaptability					46:57	environmental stress adaptability	25:57	environmental stress adaptability	25:57	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	3	21	theme	PacC	549:552	arg1	functions					536:544	the functions	532:544	the functions of PacC in G. lucidum	532:566	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	2	22	theme	lucidum	467:473	arg1	strains					428:434	PacC-silenced strains	414:434	PacC-silenced strains of the agaricomycete Ganoderma lucidum	414:473	In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed.					
31639624	4	23	theme	cell	843:846	arg1	structure					853:861	the cell wall structure	839:861	the cell wall structure	839:861	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	6	24	theme	genes	1344:1348	arg1	promoters					1306:1314	the promoters	1302:1314	the promoters of glucan synthase-encoding genes	1302:1348	The ability of PacC to bind to the promoters of glucan synthase-encoding genes confirms that PacC transcriptionally regulates these genes.					
31639624	3	25	theme	wall	702:705	arg1	stress					707:712	cell wall stress	697:712	cell wall stress	697:712	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	2	26	theme	Ganoderma	457:465	arg1	lucidum					467:473	the agaricomycete Ganoderma lucidum	439:473	the agaricomycete Ganoderma lucidum	439:473	In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed.					
31639624	5	27	theme	cell	1015:1018	arg1	composition					1025:1035	cell wall composition	1015:1035	cell wall composition	1015:1035	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	28	theme	gene	1254:1257	arg1	expression					1259:1268	glucan synthase gene expression	1238:1268	glucan synthase gene expression	1238:1268	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	2	29	theme	PacC/Rim101	382:392	arg1	gene					374:377	a homologous gene	361:377	a homologous gene of PacC/Rim101	361:392	In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed.					
31639624	2	30	theme	agaricomycete	443:455	arg1	lucidum					467:473	the agaricomycete Ganoderma lucidum	439:473	the agaricomycete Ganoderma lucidum	439:473	In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed.					
31639624	3	31	theme	empty-vector	748:759	arg1	strains					774:780	empty-vector control (CK) strains	748:780	empty-vector control (CK) strains	748:780	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	4	32	theme	PacC-silenced	897:909	arg1	strains					911:917	the PacC-silenced strains	893:917	the PacC-silenced strains	893:917	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	4	33	theme	transmission	796:807	arg1	microscopy					818:827	transmission electron microscopy	796:827	transmission electron microscopy images of the cell wall structure	796:861	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	1	34	theme	secondary	204:212	arg1	metabolism					214:223	secondary metabolism	204:223	secondary metabolism	204:223	The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear.					
31639624	0	35	theme	PacC	13:16	arg1	Influence					0:8	Influence	0:8	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.	0:104	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	1	36	theme	environmental	300:312	arg1	stress					314:319	environmental stress	300:319	environmental stress	300:319	The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear.					
31639624	6	37	theme	PacC	1286:1289	arg1	ability					1275:1281	The ability	1271:1281	The ability of PacC to bind to the promoters of glucan synthase-encoding genes	1271:1348	The ability of PacC to bind to the promoters of glucan synthase-encoding genes confirms that PacC transcriptionally regulates these genes.					
31639624	3	38	theme	osmotic	660:666	arg1	stress					668:673	osmotic stress	660:673	osmotic stress	660:673	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	0	39	theme	environmental	25:37	arg1	adaptability					46:57	environmental stress adaptability	25:57	environmental stress adaptability	25:57	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	5	40	theme	composition	1025:1035	arg1	analysis					1003:1010	Further analysis	995:1010	Further analysis of cell wall composition	995:1035	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	0	41	dep	adaptability	46:57	arg1	the					21:23	the	21:23	the	21:23	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	2	42	theme	PacC-silenced	414:426	arg1	strains					428:434	PacC-silenced strains	414:434	PacC-silenced strains of the agaricomycete Ganoderma lucidum	414:473	In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed.					
31639624	3	43	theme	CK	770:771	arg1	strains					774:780	empty-vector control (CK) strains	748:780	empty-vector control (CK) strains	748:780	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	4	44	theme	strains	911:917	arg1	walls					884:888	the cell walls	875:888	the cell walls of the PacC-silenced strains	875:917	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	4	44	theme	strains	911:917	arg1	thinner					924:930	thinner	924:930	thinner	924:930	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	1	45	theme	many	228:231	arg1	fungi					233:237	many fungi	228:237	many fungi	228:237	The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear.					
31639624	4	46	theme	WT	976:977	arg1	strains					986:992	the WT and CK strains	972:992	the WT and CK strains	972:992	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	4	47	theme	cell	879:882	arg1	walls					884:888	the cell walls	875:888	the cell walls of the PacC-silenced strains	875:917	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	4	47	theme	cell	879:882	arg1	thinner					924:930	thinner	924:930	thinner	924:930	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	5	48	theme	that	1136:1139	arg1	content					1066:1072	the β-1,3-glucan content	1049:1072	the β-1,3-glucan content in the PacC-silenced strains	1049:1101	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	48	theme	that	1136:1139	arg1	that					1136:1139	that	1136:1139	that	1136:1139	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	48	theme	that	1136:1139	arg1	%					1131:1131	only approximately 78-80%	1107:1131	only approximately 78-80% of that in the WT strain	1107:1156	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	3	49	theme	PacC-silenced	583:595	arg1	strains					597:603	PacC-silenced strains	583:603	PacC-silenced strains	583:603	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	0	50	theme	stress	39:44	arg1	adaptability					46:57	environmental stress adaptability	25:57	environmental stress adaptability	25:57	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	3	51	theme	control	761:767	arg1	strains					774:780	empty-vector control (CK) strains	748:780	empty-vector control (CK) strains	748:780	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	5	52	theme	Further	995:1001	arg1	analysis					1003:1010	Further analysis	995:1010	Further analysis of cell wall composition	995:1035	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	4	53	theme	electron	809:816	arg1	microscopy					818:827	transmission electron microscopy	796:827	transmission electron microscopy images of the cell wall structure	796:861	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	1	54	theme	transcription	110:122	arg1	factor					124:129	transcription factor	110:129	The transcription factor PacC/Rim101	106:141	The transcription factor PacC/Rim101 participates in environmental pH adaptation, development and secondary metabolism in many fungi, but whether PacC/Rim101 contributes to fungal adaptation to environmental stress remains unclear.					
31639624	5	55	from	content	1066:1072	arg1	strains					1095:1101	the PacC-silenced strains	1077:1101	the PacC-silenced strains	1077:1101	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	56	from	%	1131:1131	arg1	strain					1151:1156	the WT strain	1144:1156	the WT strain	1144:1156	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	2	57	theme	previous	345:352	arg1	study					354:358	our previous study	341:358	our previous study	341:358	In our previous study, a homologous gene of PacC/Rim101 was identified, and PacC-silenced strains of the agaricomycete Ganoderma lucidum were constructed.					
31639624	5	58	from	strain	1151:1156	arg1	content					1066:1072	the β-1,3-glucan content	1049:1072	the β-1,3-glucan content in the PacC-silenced strains	1049:1101	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	58	from	strain	1151:1156	arg1	that					1136:1139	that	1136:1139	that	1136:1139	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	58	from	strain	1151:1156	arg1	%					1131:1131	only approximately 78-80%	1107:1131	only approximately 78-80% of that in the WT strain	1107:1156	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	59	from	changes	1167:1173	arg1	content					1191:1197	β-1,3-glucan content	1178:1197	β-1,3-glucan content	1178:1197	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	60	theme	expression	1259:1268	arg1	downregulation					1220:1233	downregulation	1220:1233	downregulation of glucan synthase gene expression	1220:1268	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	5	61	theme	glucan	1238:1243	arg1	synthase					1245:1252	glucan synthase	1238:1252	glucan synthase gene expression	1238:1268	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	0	62	theme	cell	63:66	arg1	components					73:82	cell wall components	63:82	cell wall components	63:82	Influence of PacC on the environmental stress adaptability and cell wall components of Ganoderma lucidum.					
31639624	3	63	theme	oxidative	676:684	arg1	stress					686:691	oxidative stress	676:691	oxidative stress	676:691	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	5	64	theme	synthase	1245:1252	arg1	expression					1259:1268	glucan synthase gene expression	1238:1268	glucan synthase gene expression	1238:1268	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	6	65	theme	synthase-encoding	1326:1342	arg1	genes					1344:1348	glucan synthase-encoding genes	1319:1348	glucan synthase-encoding genes	1319:1348	The ability of PacC to bind to the promoters of glucan synthase-encoding genes confirms that PacC transcriptionally regulates these genes.					
31639624	5	66	with	consistent	1204:1213	arg1	downregulation					1220:1233	downregulation	1220:1233	downregulation of glucan synthase gene expression	1220:1268	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
31639624	3	67	theme	environmental	628:640	arg1	stress					707:712	cell wall stress	697:712	cell wall stress	697:712	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	3	67	theme	environmental	628:640	arg1	stress					686:691	oxidative stress	676:691	oxidative stress	676:691	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	3	67	theme	environmental	628:640	arg1	stress					668:673	osmotic stress	660:673	osmotic stress	660:673	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	3	67	theme	environmental	628:640	arg1	stresses					642:649	environmental stresses	628:649	environmental stresses	628:649	In this study, we further investigated the functions of PacC in G. lucidum and found that PacC-silenced strains were hypersensitive to environmental stresses, such as osmotic stress, oxidative stress and cell wall stress, compared with wild-type (WT) and empty-vector control (CK) strains.					
31639624	4	68	theme	CK	983:984	arg1	strains					986:992	the WT and CK strains	972:992	the WT and CK strains	972:992	In addition, transmission electron microscopy images of the cell wall structure showed that the cell walls of the PacC-silenced strains were thinner (by approximately 25-30%) than those of the WT and CK strains.					
31639624	5	69	theme	β-1,3-glucan	1178:1189	arg1	content					1191:1197	β-1,3-glucan content	1178:1197	β-1,3-glucan content	1178:1197	Further analysis of cell wall composition showed that the β-1,3-glucan content in the PacC-silenced strains was only approximately 78-80% of that in the WT strain, and the changes in β-1,3-glucan content were consistent with downregulation of glucan synthase gene expression.					
30155669	5	0	dep	Fourier	1111:1117	arg1	transform					1119:1127	transform	1119:1127	transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy	1119:1292	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	10	1	theme	multifunctional	2084:2098	arg1	coatings					2100:2107	such biomimetic multifunctional coatings	2068:2107	such biomimetic multifunctional coatings	2068:2107	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	10	2	theme	biomimetic	2073:2082	arg1	coatings					2100:2107	such biomimetic multifunctional coatings	2068:2107	such biomimetic multifunctional coatings	2068:2107	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	6	3	theme	Mg	1350:1351	arg1	alloy					1353:1357	Mg alloy	1350:1357	Mg alloy	1350:1357	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	5	4	theme	AZ31	1280:1283	arg1	Mg					1285:1286	Mg	1285:1286	Mg	1285:1286	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	7	5	theme	substrate	1574:1582	arg1	resistance					1523:1532	an enhanced corrosion resistance	1501:1532	an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate	1501:1582	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	8	6	theme	total	1768:1772	arg1	content					1787:1793	total cellular DNA content	1768:1793	total cellular DNA content	1768:1793	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	10	7	theme	corrosion	2124:2132	arg1	resistance					2134:2143	corrosion resistance	2124:2143	corrosion resistance	2124:2143	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	3	8	theme	coatings	568:575	arg1	fabrication					553:563	The multi-step fabrication	538:563	The multi-step fabrication of coatings	538:575	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	8	9	theme	phosphatase	1838:1848	arg1	activity					1850:1857	alkaline phosphatase activity	1829:1857	alkaline phosphatase activity	1829:1857	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	7	10	theme	coated	1562:1567	arg1	substrate					1574:1582	HA functionalised silane coated AZ31 substrate	1537:1582	HA functionalised silane coated AZ31 substrate	1537:1582	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	7	11	theme	enhanced	1504:1511	arg1	resistance					1523:1532	an enhanced corrosion resistance	1501:1532	an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate	1501:1582	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	1	12	theme	resistant	192:200	arg1	properties					221:230	the corrosion resistant and cytocompatible properties	178:230	the corrosion resistant and cytocompatible properties of the hyaluronic acid-silane coating on AZ31 Mg alloy	178:285	This paper reports the corrosion resistant and cytocompatible properties of the hyaluronic acid-silane coating on AZ31 Mg alloy.					
30155669	7	13	theme	functionalised	1540:1553	arg1	substrate					1574:1582	HA functionalised silane coated AZ31 substrate	1537:1582	HA functionalised silane coated AZ31 substrate	1537:1582	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	6	14	theme	HA-silane	1312:1320	arg1	coating					1322:1328	HA-silane coating	1312:1328	HA-silane coating	1312:1328	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	1	15	theme	cytocompatible	206:219	arg1	properties					221:230	the corrosion resistant and cytocompatible properties	178:230	the corrosion resistant and cytocompatible properties of the hyaluronic acid-silane coating on AZ31 Mg alloy	178:285	This paper reports the corrosion resistant and cytocompatible properties of the hyaluronic acid-silane coating on AZ31 Mg alloy.					
30155669	2	16	theme	osteoinductive	307:320	arg1	properties					322:331	the osteoinductive properties	303:331	the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa)	303:386	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	16	theme	osteoinductive	307:320	arg1	protection					406:415	the corrosion protection	392:415	the corrosion protection	392:415	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	16	theme	osteoinductive	307:320	arg1	coating					469:475	a composite coating	457:475	a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications	457:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	6	17	dep	spectroscopy	1400:1411	arg1	measurements					1445:1456	measurements	1445:1456	measurements	1445:1456	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	6	18	theme	impedance	1390:1398	arg1	spectroscopy					1400:1411	electrical impedance spectroscopy	1379:1411	electrical impedance spectroscopy	1379:1411	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	3	19	theme	AZ31	620:623	arg1	Mg					625:626	Mg	625:626	Mg	625:626	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	1	20	theme	hyaluronic	239:248	arg1	acid-silane					250:260	the hyaluronic acid-silane	235:260	the hyaluronic acid-silane coating on AZ31 Mg alloy	235:285	This paper reports the corrosion resistant and cytocompatible properties of the hyaluronic acid-silane coating on AZ31 Mg alloy.					
30155669	4	21	theme	HA	947:948	arg1	immobilisation					929:942	the immobilisation	925:942	the immobilisation of HA	925:948	The second step was to create an amine-functionalised surface by treating coated alloy with 3-aminopropyl-triethoxy silane (AZ31-MT-A) which facilitated the immobilisation of HA via EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2).					
30155669	10	22	theme	Mg-based	2184:2191	arg1	alloy					2193:2197	biodegradable Mg-based alloy	2170:2197	biodegradable Mg-based alloy for orthopaedic applications	2170:2226	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	4	23	theme	EDC-NHS	954:960	arg1	reactions					971:979	EDC-NHS coupling reactions	954:979	EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2)	954:1071	The second step was to create an amine-functionalised surface by treating coated alloy with 3-aminopropyl-triethoxy silane (AZ31-MT-A) which facilitated the immobilisation of HA via EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2).					
30155669	5	24	theme	Mg	1285:1286	arg1	alloy					1288:1292	AZ31 Mg alloy	1280:1292	AZ31 Mg alloy	1280:1292	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	9	25	theme	concentration-dependent	1882:1904	arg1	improvement					1906:1916	HA concentration-dependent improvement	1879:1916	HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation	1879:2018	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	6	26	from	influence	1299:1307	arg1	corrosion					1337:1345	the corrosion	1333:1345	the corrosion of Mg alloy	1333:1357	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	3	27	with	alloy	628:632	arg1	sol-gel					681:687	a methyltriethoxysilane-tetraethoxysilane sol-gel	639:687	a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	639:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	5	28	theme	contact	1187:1193	arg1	measurements					1201:1212	static contact angle measurements	1180:1212	static contact angle measurements	1180:1212	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	3	29	theme	alloy	628:632	arg1	coating					596:602	dip coating	592:602	dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	592:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	2	30	theme	Mg	499:500	arg1	alloy					502:506	biodegradable AZ31 Mg alloy	480:506	biodegradable AZ31 Mg alloy for orthopaedic applications	480:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	9	31	theme	osteoblast	1921:1930	arg1	response					1941:1948	osteoblast cellular response	1921:1948	osteoblast cellular response	1921:1948	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	0	32	theme	silane	97:102	arg1	coating					104:110	silane coating	97:110	silane coating	97:110	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	9	33	theme	cellular	1932:1939	arg1	response					1941:1948	osteoblast cellular response	1921:1948	osteoblast cellular response	1921:1948	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	5	34	theme	force	1159:1163	arg1	microscopy					1165:1174	atomic force microscopy	1152:1174	atomic force microscopy	1152:1174	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	10	35	dep	resistance	2134:2143	arg1	the					2120:2122	the	2120:2122	the	2120:2122	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	2	36	theme	corrosion	396:404	arg1	coating					469:475	a composite coating	457:475	a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications	457:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	36	theme	corrosion	396:404	arg1	protection					406:415	the corrosion protection	392:415	the corrosion protection	392:415	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	36	theme	corrosion	396:404	arg1	properties					322:331	the osteoinductive properties	303:331	the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa)	303:386	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	0	37	theme	Mg	120:121	arg1	alloy					123:127	AZ31 Mg alloy	115:127	AZ31 Mg alloy for orthopaedic applications	115:156	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	2	38	dep	weight	351:356	arg1	acid					369:372	hyaluronic acid	358:372	high molecular weight hyaluronic acid (HA, 1-4 MDa)	336:386	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	0	39	theme	Enhanced	0:7	arg1	resistance					19:28	Enhanced corrosion resistance	0:28	Enhanced corrosion resistance	0:28	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	9	40	theme	cell	1971:1974	arg1	adhesion					1976:1983	enhanced cell adhesion	1962:1983	enhanced cell adhesion	1962:1983	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	2	41	dep	acid	369:372	arg1	HA					375:376	HA	375:376	HA	375:376	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	41	dep	acid	369:372	arg1	1-4 MDa					379:385	1-4 MDa	379:385	1-4 MDa	379:385	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	42	theme	coatings	427:434	arg1	properties					322:331	the osteoinductive properties	303:331	the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa)	303:386	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	42	theme	coatings	427:434	arg1	protection					406:415	the corrosion protection	392:415	the corrosion protection	392:415	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	42	theme	coatings	427:434	arg1	coating					469:475	a composite coating	457:475	a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications	457:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	5	43	theme	full	1261:1264	arg1	coatings					1266:1273	the full coatings	1257:1273	the full coatings	1257:1273	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	10	44	theme	great	2041:2045	arg1	promise					2047:2053	great promise	2041:2053	great promise	2041:2053	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	2	45	theme	biodegradable	480:492	arg1	alloy					502:506	biodegradable AZ31 Mg alloy	480:506	biodegradable AZ31 Mg alloy for orthopaedic applications	480:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	8	46	theme	osteoblasts	1667:1677	arg1	cytocompatibility					1637:1653	the cytocompatibility	1633:1653	the cytocompatibility of MC3T3-E1 osteoblasts	1633:1677	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	0	47	theme	acid	77:80	arg1	resistance					19:28	Enhanced corrosion resistance	0:28	Enhanced corrosion resistance	0:28	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	0	47	theme	acid	77:80	arg1	cytocompatibility					34:50	cytocompatibility	34:50	cytocompatibility of biomimetic hyaluronic acid	34:80	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	0	48	theme	biomimetic	55:64	arg1	acid					77:80	biomimetic hyaluronic acid	55:80	biomimetic hyaluronic acid	55:80	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	5	49	theme	successful	1234:1243	arg1	assembly					1245:1252	the successful assembly	1230:1252	the successful assembly of the full coatings onto AZ31 Mg alloy	1230:1292	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	8	50	theme	HA-coated	1696:1704	arg1	substrates					1716:1725	HA-coated AZ31-MT-A substrates	1696:1725	HA-coated AZ31-MT-A substrates	1696:1725	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	3	51	theme	cross-linked	709:720	arg1	AZ31-MT					762:768	AZ31-MT	762:768	AZ31-MT	762:768	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	3	51	theme	cross-linked	709:720	arg1	coating					753:759	a dense, cross-linked and corrosion resistant silane coating	700:759	a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	700:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	2	52	theme	weight	351:356	arg1	properties					322:331	the osteoinductive properties	303:331	the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa)	303:386	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	52	theme	weight	351:356	arg1	protection					406:415	the corrosion protection	392:415	the corrosion protection	392:415	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	52	theme	weight	351:356	arg1	coating					469:475	a composite coating	457:475	a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications	457:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	8	53	theme	cellular	1774:1781	arg1	content					1787:1793	total cellular DNA content	1768:1793	total cellular DNA content	1768:1793	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	1	54	theme	acid-silane	250:260	arg1	properties					221:230	the corrosion resistant and cytocompatible properties	178:230	the corrosion resistant and cytocompatible properties of the hyaluronic acid-silane coating on AZ31 Mg alloy	178:285	This paper reports the corrosion resistant and cytocompatible properties of the hyaluronic acid-silane coating on AZ31 Mg alloy.					
30155669	7	55	theme	uncoated	1593:1600	arg1	alloy					1613:1617	the uncoated equivalent alloy	1589:1617	the uncoated equivalent alloy	1589:1617	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	3	56	theme	dense	702:706	arg1	AZ31-MT					762:768	AZ31-MT	762:768	AZ31-MT	762:768	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	3	56	theme	dense	702:706	arg1	coating					753:759	a dense, cross-linked and corrosion resistant silane coating	700:759	a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	700:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	8	57	theme	content	1787:1793	arg1	activity					1850:1857	alkaline phosphatase activity	1829:1857	alkaline phosphatase activity	1829:1857	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	8	57	theme	content	1787:1793	arg1	quantification					1750:1763	quantification	1750:1763	quantification of total cellular DNA content	1750:1793	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	8	57	theme	content	1787:1793	arg1	staining					1740:1747	live-dead staining	1730:1747	live-dead staining	1730:1747	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	8	57	theme	content	1787:1793	arg1	microscope					1814:1823	scanning electron microscope	1796:1823	scanning electron microscope	1796:1823	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	10	58	theme	orthopaedic	2203:2213	arg1	applications					2215:2226	orthopaedic applications	2203:2226	orthopaedic applications	2203:2226	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	8	59	theme	live-dead	1730:1738	arg1	staining					1740:1747	live-dead staining	1730:1747	live-dead staining	1730:1747	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	3	60	theme	silane	746:751	arg1	AZ31-MT					762:768	AZ31-MT	762:768	AZ31-MT	762:768	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	3	60	theme	silane	746:751	arg1	coating					753:759	a dense, cross-linked and corrosion resistant silane coating	700:759	a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	700:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	6	61	theme	alloy	1353:1357	arg1	corrosion					1337:1345	the corrosion	1333:1345	the corrosion of Mg alloy	1333:1357	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	8	62	theme	scanning	1796:1803	arg1	microscope					1814:1823	scanning electron microscope	1796:1823	scanning electron microscope	1796:1823	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	1	63	theme	Mg	278:279	arg1	alloy					281:285	AZ31 Mg alloy	273:285	AZ31 Mg alloy	273:285	This paper reports the corrosion resistant and cytocompatible properties of the hyaluronic acid-silane coating on AZ31 Mg alloy.					
30155669	7	64	theme	AZ31	1569:1572	arg1	substrate					1574:1582	HA functionalised silane coated AZ31 substrate	1537:1582	HA functionalised silane coated AZ31 substrate	1537:1582	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	5	65	dep	transform	1119:1127	arg1	infrared					1129:1136	infrared	1129:1136	transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy	1119:1292	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	10	66	theme	such	2068:2071	arg1	coatings					2100:2107	such biomimetic multifunctional coatings	2068:2107	such biomimetic multifunctional coatings	2068:2107	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	4	67	from	concentrations	998:1011	arg1	reactions					971:979	EDC-NHS coupling reactions	954:979	EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2)	954:1071	The second step was to create an amine-functionalised surface by treating coated alloy with 3-aminopropyl-triethoxy silane (AZ31-MT-A) which facilitated the immobilisation of HA via EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2).					
30155669	3	68	theme	dip	592:594	arg1	coating					596:602	dip coating	592:602	dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	592:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	7	69	theme	silane	1555:1560	arg1	substrate					1574:1582	HA functionalised silane coated AZ31 substrate	1537:1582	HA functionalised silane coated AZ31 substrate	1537:1582	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	3	70	theme	multi-step	542:551	arg1	fabrication					553:563	The multi-step fabrication	538:563	The multi-step fabrication of coatings	538:575	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	8	71	theme	alkaline	1829:1836	arg1	phosphatase					1838:1848	alkaline phosphatase	1829:1848	alkaline phosphatase activity	1829:1857	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	9	72	theme	differentiation	2004:2018	arg1	terms					1953:1957	terms	1953:1957	terms of enhanced cell adhesion, proliferation and differentiation	1953:2018	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	7	73	theme	HA	1537:1538	arg1	substrate					1574:1582	HA functionalised silane coated AZ31 substrate	1537:1582	HA functionalised silane coated AZ31 substrate	1537:1582	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	7	74	theme	corrosion	1513:1521	arg1	resistance					1523:1532	an enhanced corrosion resistance	1501:1532	an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate	1501:1582	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	6	75	theme	HEPES	1461:1465	arg1	DMEM					1476:1479	HEPES buffered DMEM	1461:1479	HEPES buffered DMEM	1461:1479	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	6	76	theme	coating	1322:1328	arg1	influence					1299:1307	The influence	1295:1307	The influence of HA-silane coating on the corrosion of Mg alloy	1295:1357	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	2	77	theme	high	336:339	arg1	weight					351:356	high molecular weight	336:356	high molecular weight hyaluronic acid (HA, 1-4 MDa)	336:386	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	6	78	theme	long-term	1417:1425	arg1	studies					1437:1443	long-term immersion studies	1417:1443	long-term immersion studies	1417:1443	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	4	79	theme	coupling	962:969	arg1	reactions					971:979	EDC-NHS coupling reactions	954:979	EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2)	954:1071	The second step was to create an amine-functionalised surface by treating coated alloy with 3-aminopropyl-triethoxy silane (AZ31-MT-A) which facilitated the immobilisation of HA via EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2).					
30155669	3	80	theme	passivated	609:618	arg1	alloy					628:632	a passivated AZ31 Mg alloy	607:632	a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	607:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	10	81	theme	alloy	2193:2197	arg1	cytocompatibility					2149:2165	cytocompatibility	2149:2165	cytocompatibility	2149:2165	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	10	81	theme	alloy	2193:2197	arg1	resistance					2134:2143	corrosion resistance	2124:2143	corrosion resistance	2124:2143	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	6	82	theme	electrical	1379:1388	arg1	spectroscopy					1400:1411	electrical impedance spectroscopy	1379:1411	electrical impedance spectroscopy	1379:1411	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	5	83	theme	atomic	1152:1157	arg1	microscopy					1165:1174	atomic force microscopy	1152:1174	atomic force microscopy	1152:1174	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	0	84	theme	orthopaedic	133:143	arg1	applications					145:156	orthopaedic applications	133:156	orthopaedic applications	133:156	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	10	85	theme	biodegradable	2170:2182	arg1	alloy					2193:2197	biodegradable Mg-based alloy	2170:2197	biodegradable Mg-based alloy for orthopaedic applications	2170:2226	These findings hold great promise in employing such biomimetic multifunctional coatings to improve the corrosion resistance and cytocompatibility of biodegradable Mg-based alloy for orthopaedic applications.					
30155669	9	86	theme	HA	1879:1880	arg1	improvement					1906:1916	HA concentration-dependent improvement	1879:1916	HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation	1879:2018	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	3	87	theme	Mg	625:626	arg1	alloy					628:632	a passivated AZ31 Mg alloy	607:632	a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	607:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	9	88	theme	response	1941:1948	arg1	improvement					1906:1916	HA concentration-dependent improvement	1879:1916	HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation	1879:2018	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	6	89	theme	buffered	1467:1474	arg1	DMEM					1476:1479	HEPES buffered DMEM	1461:1479	HEPES buffered DMEM	1461:1479	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	5	90	theme	angle	1195:1199	arg1	measurements					1201:1212	static contact angle measurements	1180:1212	static contact angle measurements	1180:1212	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	2	91	theme	AZ31	494:497	arg1	alloy					502:506	biodegradable AZ31 Mg alloy	480:506	biodegradable AZ31 Mg alloy for orthopaedic applications	480:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	0	92	theme	AZ31	115:118	arg1	Mg					120:121	Mg	120:121	Mg	120:121	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	4	93	theme	different	988:996	arg1	concentrations					998:1011	two different concentrations	984:1011	two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2)	984:1071	The second step was to create an amine-functionalised surface by treating coated alloy with 3-aminopropyl-triethoxy silane (AZ31-MT-A) which facilitated the immobilisation of HA via EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2).					
30155669	2	94	from	coating	469:475	arg1	alloy					502:506	biodegradable AZ31 Mg alloy	480:506	biodegradable AZ31 Mg alloy for orthopaedic applications	480:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	95	theme	orthopaedic	512:522	arg1	applications					524:535	orthopaedic applications	512:535	orthopaedic applications	512:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	0	96	theme	corrosion	9:17	arg1	resistance					19:28	Enhanced corrosion resistance	0:28	Enhanced corrosion resistance	0:28	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	5	97	theme	static	1180:1185	arg1	measurements					1201:1212	static contact angle measurements	1180:1212	static contact angle measurements	1180:1212	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	9	98	theme	enhanced	1962:1969	arg1	adhesion					1976:1983	enhanced cell adhesion	1962:1983	enhanced cell adhesion	1962:1983	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	8	99	theme	MC3T3-E1	1658:1665	arg1	osteoblasts					1667:1677	MC3T3-E1 osteoblasts	1658:1677	MC3T3-E1 osteoblasts	1658:1677	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	9	100	from	improvement	1906:1916	arg1	terms					1953:1957	terms	1953:1957	terms of enhanced cell adhesion, proliferation and differentiation	1953:2018	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	9	101	theme	adhesion	1976:1983	arg1	terms					1953:1957	terms	1953:1957	terms of enhanced cell adhesion, proliferation and differentiation	1953:2018	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	4	102	theme	second	776:781	arg1	step					783:786	The second step	772:786	The second step	772:786	The second step was to create an amine-functionalised surface by treating coated alloy with 3-aminopropyl-triethoxy silane (AZ31-MT-A) which facilitated the immobilisation of HA via EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2).					
30155669	2	103	theme	silane	420:425	arg1	coatings					427:434	silane coatings	420:434	silane coatings	420:434	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	5	104	theme	coatings	1266:1273	arg1	assembly					1245:1252	the successful assembly	1230:1252	the successful assembly of the full coatings onto AZ31 Mg alloy	1230:1292	These coatings were characterised by Fourier transform infrared spectroscopy, atomic force microscopy and static contact angle measurements which confirmed the successful assembly of the full coatings onto AZ31 Mg alloy.					
30155669	9	105	theme	proliferation	1986:1998	arg1	terms					1953:1957	terms	1953:1957	terms of enhanced cell adhesion, proliferation and differentiation	1953:2018	The results showed HA concentration-dependent improvement of osteoblast cellular response in terms of enhanced cell adhesion, proliferation and differentiation.					
30155669	3	106	theme	methyltriethoxysilane-tetraethoxysilane	641:679	arg1	sol-gel					681:687	a methyltriethoxysilane-tetraethoxysilane sol-gel	639:687	a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	639:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	4	107	theme	coated	846:851	arg1	alloy					853:857	coated alloy	846:857	coated alloy	846:857	The second step was to create an amine-functionalised surface by treating coated alloy with 3-aminopropyl-triethoxy silane (AZ31-MT-A) which facilitated the immobilisation of HA via EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2).					
30155669	6	108	theme	immersion	1427:1435	arg1	studies					1437:1443	long-term immersion studies	1417:1443	long-term immersion studies	1417:1443	The influence of HA-silane coating on the corrosion of Mg alloy was investigated by electrical impedance spectroscopy and long-term immersion studies measurements in HEPES buffered DMEM.					
30155669	0	109	theme	hyaluronic	66:75	arg1	acid					77:80	biomimetic hyaluronic acid	55:80	biomimetic hyaluronic acid	55:80	Enhanced corrosion resistance and cytocompatibility of biomimetic hyaluronic acid functionalised silane coating on AZ31 Mg alloy for orthopaedic applications.					
30155669	2	110	theme	composite	459:467	arg1	coating					469:475	a composite coating	457:475	a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications	457:535	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	110	theme	composite	459:467	arg1	protection					406:415	the corrosion protection	392:415	the corrosion protection	392:415	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	2	110	theme	composite	459:467	arg1	properties					322:331	the osteoinductive properties	303:331	the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa)	303:386	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	8	111	theme	AZ31-MT-A	1706:1714	arg1	substrates					1716:1725	HA-coated AZ31-MT-A substrates	1696:1725	HA-coated AZ31-MT-A substrates	1696:1725	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	2	112	theme	hyaluronic	358:367	arg1	acid					369:372	hyaluronic acid	358:372	high molecular weight hyaluronic acid (HA, 1-4 MDa)	336:386	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	8	113	theme	DNA	1783:1785	arg1	content					1787:1793	total cellular DNA content	1768:1793	total cellular DNA content	1768:1793	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	4	114	theme	amine-functionalised	805:824	arg1	surface					826:832	an amine-functionalised surface	802:832	an amine-functionalised surface	802:832	The second step was to create an amine-functionalised surface by treating coated alloy with 3-aminopropyl-triethoxy silane (AZ31-MT-A) which facilitated the immobilisation of HA via EDC-NHS coupling reactions at two different concentrations i.e 1 mg.ml-1 (AZ31-MT-A-HA1) and 2 mg.ml-1 (AZ31-MT-A-HA2).					
30155669	2	115	theme	molecular	341:349	arg1	weight					351:356	high molecular weight	336:356	high molecular weight hyaluronic acid (HA, 1-4 MDa)	336:386	In this study, the osteoinductive properties of high molecular weight hyaluronic acid (HA, 1-4 MDa) and the corrosion protection of silane coatings were incorporated as a composite coating on biodegradable AZ31 Mg alloy for orthopaedic applications.					
30155669	7	116	theme	equivalent	1602:1611	arg1	alloy					1613:1617	the uncoated equivalent alloy	1589:1617	the uncoated equivalent alloy	1589:1617	The results showed an enhanced corrosion resistance of HA functionalised silane coated AZ31 substrate over the uncoated equivalent alloy.					
30155669	3	117	link	cross-linked	709:720	arg1	AZ31-MT					762:768	AZ31-MT	762:768	AZ31-MT	762:768	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	3	117	link	cross-linked	709:720	arg1	coating					753:759	a dense, cross-linked and corrosion resistant silane coating	700:759	a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	700:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	3	118	theme	resistant	736:744	arg1	AZ31-MT					762:768	AZ31-MT	762:768	AZ31-MT	762:768	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	3	118	theme	resistant	736:744	arg1	coating					753:759	a dense, cross-linked and corrosion resistant silane coating	700:759	a dense, cross-linked and corrosion resistant silane coating (AZ31-MT)	700:769	The multi-step fabrication of coatings first involved dip coating of a passivated AZ31 Mg alloy with a methyltriethoxysilane-tetraethoxysilane sol-gel to deposit a dense, cross-linked and corrosion resistant silane coating (AZ31-MT).					
30155669	8	119	theme	electron	1805:1812	arg1	microscope					1814:1823	scanning electron microscope	1796:1823	scanning electron microscope	1796:1823	Furthermore, the cytocompatibility of MC3T3-E1 osteoblasts was evaluated on HA-coated AZ31-MT-A substrates by live-dead staining, quantification of total cellular DNA content, scanning electron microscope and alkaline phosphatase activity.					
30155669	1	120	theme	AZ31	273:276	arg1	Mg					278:279	Mg	278:279	Mg	278:279	This paper reports the corrosion resistant and cytocompatible properties of the hyaluronic acid-silane coating on AZ31 Mg alloy.					
30184788	0	0	theme	enhanced	100:107	arg1	properties					120:129	enhanced mechanical properties	100:129	enhanced mechanical properties for tissue engineering	100:152	Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering.					
30184788	1	1	theme	poor	260:263	arg1	properties					276:285	the poor mechanical properties	256:285	the poor mechanical properties of pure gelatin or CS hydrogels	256:317	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	4	2	theme	hydrogel	977:984	arg1	network					986:992	the hydrogel network	973:992	the hydrogel network	973:992	Moreover, the increase of either GelMA or CS concentration can strengthen the hydrogel network.					
30184788	3	3	theme	network	878:884	arg1	CS					886:887	the single network CS	867:887	the single network CS	867:887	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	1	4	theme	mechanical	265:274	arg1	properties					276:285	the poor mechanical properties	256:285	the poor mechanical properties of pure gelatin or CS hydrogels	256:317	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	4	5	theme	CS	941:942	arg1	increase					913:920	the increase	909:920	the increase of either GelMA or CS concentration	909:956	Moreover, the increase of either GelMA or CS concentration can strengthen the hydrogel network.					
30184788	3	6	theme	ultimate	706:713	arg1	stress					723:728	ultimate tensile stress	706:728	ultimate tensile stress	706:728	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	3	6	theme	ultimate	706:713	arg1	moduli					698:703	the compressive and tensile moduli	670:703	the compressive and tensile moduli	670:703	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	6	7	theme	hydrogel	1242:1249	arg1	surfaces					1251:1258	hydrogel surfaces	1242:1258	hydrogel surfaces	1242:1258	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	0	8	theme	mechanical	109:118	arg1	properties					120:129	enhanced mechanical properties	100:129	enhanced mechanical properties for tissue engineering	100:152	Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering.					
30184788	7	9	theme	IPN	1436:1438	arg1	hydrogels					1440:1448	IPN hydrogels	1436:1448	IPN hydrogels	1436:1448	According to this study, the multiple requirements of properties can be fulfilled by carefully selecting the GelMA and CS compositions for IPN hydrogels.					
30184788	0	10	theme	tissue	135:140	arg1	engineering					142:152	tissue engineering	135:152	tissue engineering	135:152	Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering.					
30184788	0	11	with	photocrosslinkable	68:85	arg1	properties					120:129	enhanced mechanical properties	100:129	enhanced mechanical properties for tissue engineering	100:152	Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering.					
30184788	6	12	theme	spreading	1193:1201	arg1	area					1203:1206	large spreading area	1187:1206	large spreading area of bone mesenchymal stem cells on hydrogel surfaces	1187:1258	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	2	13	theme	methacryloyl	504:515	arg1	hydrogels					532:540	CS hydrogels	529:540	CS hydrogels	529:540	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	2	13	theme	methacryloyl	504:515	arg1	GelMA					518:522	GelMA	518:522	GelMA	518:522	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	2	13	theme	methacryloyl	504:515	arg1	IPN					488:490	IPN	488:490	IPN	488:490	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	2	13	theme	methacryloyl	504:515	arg1	network					479:485	interpenetrating network	462:485	interpenetrating network (IPN) of gelatin methacryloyl (GelMA)	462:523	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	3	14	theme	GelMA-CS	836:843	arg1	hydrogels					845:853	GelMA-CS hydrogels	836:853	GelMA-CS hydrogels	836:853	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	6	15	theme	large	1187:1191	arg1	area					1203:1206	large spreading area	1187:1206	large spreading area of bone mesenchymal stem cells on hydrogel surfaces	1187:1258	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	7	16	theme	multiple	1326:1333	arg1	requirements					1335:1346	the multiple requirements	1322:1346	the multiple requirements of properties	1322:1360	According to this study, the multiple requirements of properties can be fulfilled by carefully selecting the GelMA and CS compositions for IPN hydrogels.					
30184788	0	17	theme	polymer	17:23	arg1	hydrogels					33:41	polymer network hydrogels	17:41	polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering	17:152	Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering.					
30184788	7	18	theme	GelMA	1406:1410	arg1	compositions					1419:1430	the GelMA and CS compositions	1402:1430	the GelMA and CS compositions for IPN hydrogels	1402:1448	According to this study, the multiple requirements of properties can be fulfilled by carefully selecting the GelMA and CS compositions for IPN hydrogels.					
30184788	6	19	from	area	1203:1206	arg1	surfaces					1251:1258	hydrogel surfaces	1242:1258	hydrogel surfaces	1242:1258	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	1	20	theme	used	192:195	arg1	Gelatin					155:161	Gelatin	155:161	Gelatin	155:161	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	1	20	theme	used	192:195	arg1	chitosan					167:174	chitosan	167:174	chitosan (CS)	167:179	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	1	20	theme	used	192:195	arg1	biomaterials					205:216	widely used natural biomaterials	185:216	widely used natural biomaterials for tissue engineering scaffolds	185:249	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	4	21	theme	GelMA	932:936	arg1	increase					913:920	the increase	909:920	the increase of either GelMA or CS concentration	909:956	Moreover, the increase of either GelMA or CS concentration can strengthen the hydrogel network.					
30184788	1	22	theme	pure	290:293	arg1	hydrogels					309:317	pure gelatin or CS hydrogels	290:317	pure gelatin or CS hydrogels	290:317	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	6	23	theme	CS	1265:1266	arg1	concentration					1268:1280	CS concentration	1265:1280	CS concentration	1265:1280	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	7	24	theme	properties	1351:1360	arg1	requirements					1335:1346	the multiple requirements	1322:1346	the multiple requirements of properties	1322:1360	According to this study, the multiple requirements of properties can be fulfilled by carefully selecting the GelMA and CS compositions for IPN hydrogels.					
30184788	1	25	theme	natural	197:203	arg1	Gelatin					155:161	Gelatin	155:161	Gelatin	155:161	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	1	25	theme	natural	197:203	arg1	chitosan					167:174	chitosan	167:174	chitosan (CS)	167:179	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	1	25	theme	natural	197:203	arg1	biomaterials					205:216	widely used natural biomaterials	185:216	widely used natural biomaterials for tissue engineering scaffolds	185:249	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	1	26	from	use	359:361	arg1	tissues					404:410	load-bearing tissues	391:410	load-bearing tissues	391:410	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	1	27	theme	gelatin	295:301	arg1	hydrogels					309:317	pure gelatin or CS hydrogels	290:317	pure gelatin or CS hydrogels	290:317	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	0	28	theme	network	25:31	arg1	hydrogels					33:41	polymer network hydrogels	17:41	polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering	17:152	Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering.					
30184788	7	29	theme	CS	1416:1417	arg1	compositions					1419:1430	the GelMA and CS compositions	1402:1430	the GelMA and CS compositions for IPN hydrogels	1402:1448	According to this study, the multiple requirements of properties can be fulfilled by carefully selecting the GelMA and CS compositions for IPN hydrogels.					
30184788	5	30	theme	GelMA-CS	1056:1063	arg1	hydrogels					1065:1073	GelMA-CS hydrogels	1056:1073	GelMA-CS hydrogels	1056:1073	Then, the swelling, enzymatic degradation, and morphology of GelMA-CS hydrogels were also systematically investigated.					
30184788	6	31	theme	hydrogels	1157:1165	arg1	biocompatibility					1128:1143	The excellent biocompatibility	1114:1143	The excellent biocompatibility of GelMA-CS hydrogels	1114:1165	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	3	32	theme	IPN	773:775	arg1	structure					777:785	IPN structure	773:785	IPN structure	773:785	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	1	33	theme	CS	306:307	arg1	hydrogels					309:317	pure gelatin or CS hydrogels	290:317	pure gelatin or CS hydrogels	290:317	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	2	34	theme	CS	529:530	arg1	hydrogels					532:540	CS hydrogels	529:540	CS hydrogels	529:540	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	5	35	theme	hydrogels	1065:1073	arg1	morphology					1042:1051	morphology	1042:1051	morphology	1042:1051	Then, the swelling, enzymatic degradation, and morphology of GelMA-CS hydrogels were also systematically investigated.					
30184788	5	35	theme	hydrogels	1065:1073	arg1	swelling					1005:1012	the swelling	1001:1012	the swelling	1001:1012	Then, the swelling, enzymatic degradation, and morphology of GelMA-CS hydrogels were also systematically investigated.					
30184788	5	35	theme	hydrogels	1065:1073	arg1	degradation					1025:1035	enzymatic degradation	1015:1035	enzymatic degradation	1015:1035	Then, the swelling, enzymatic degradation, and morphology of GelMA-CS hydrogels were also systematically investigated.					
30184788	1	36	theme	tissue	222:227	arg1	scaffolds					241:249	tissue engineering scaffolds	222:249	tissue engineering scaffolds	222:249	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	1	37	theme	hydrogels	309:317	arg1	properties					276:285	the poor mechanical properties	256:285	the poor mechanical properties of pure gelatin or CS hydrogels	256:317	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	3	38	theme	hydrogels	845:853	arg1	properties					822:831	the mechanical properties	807:831	the mechanical properties of GelMA-CS hydrogels	807:853	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	1	39	theme	load-bearing	391:402	arg1	tissues					404:410	load-bearing tissues	391:410	load-bearing tissues	391:410	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	0	40	dep	chitosan	55:62	arg1	gelatin					87:93	gelatin	87:93	gelatin	87:93	Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering.					
30184788	1	41	theme	engineering	229:239	arg1	scaffolds					241:249	tissue engineering scaffolds	222:249	tissue engineering scaffolds	222:249	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	2	42	theme	interpenetrating	462:477	arg1	GelMA					518:522	GelMA	518:522	GelMA	518:522	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	2	42	theme	interpenetrating	462:477	arg1	IPN					488:490	IPN	488:490	IPN	488:490	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	2	42	theme	interpenetrating	462:477	arg1	network					479:485	interpenetrating network	462:485	interpenetrating network (IPN) of gelatin methacryloyl (GelMA)	462:523	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	4	43	dep	GelMA	932:936	arg1	concentration					944:956	concentration	944:956	concentration	944:956	Moreover, the increase of either GelMA or CS concentration can strengthen the hydrogel network.					
30184788	2	44	theme	hydrophobic	564:574	arg1	interactions					576:587	hydrophobic interactions	564:587	hydrophobic interactions	564:587	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	5	45	theme	enzymatic	1015:1023	arg1	degradation					1025:1035	enzymatic degradation	1015:1035	enzymatic degradation	1015:1035	Then, the swelling, enzymatic degradation, and morphology of GelMA-CS hydrogels were also systematically investigated.					
30184788	6	46	theme	GelMA-CS	1148:1155	arg1	hydrogels					1157:1165	GelMA-CS hydrogels	1148:1165	GelMA-CS hydrogels	1148:1165	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	2	47	theme	novel	435:439	arg1	mechanism					441:449	a novel mechanism	433:449	a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively	433:644	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	3	48	theme	tensile	690:696	arg1	strain					734:739	strain	734:739	strain	734:739	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	3	48	theme	tensile	690:696	arg1	stress					723:728	ultimate tensile stress	706:728	ultimate tensile stress	706:728	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	3	48	theme	tensile	690:696	arg1	moduli					698:703	the compressive and tensile moduli	670:703	the compressive and tensile moduli	670:703	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	1	49	theme	big	328:330	arg1	obstacle					332:339	a big obstacle	326:339	a big obstacle that limits their use as scaffolds, especially in load-bearing tissues	326:410	Gelatin and chitosan (CS) are widely used natural biomaterials for tissue engineering scaffolds, but the poor mechanical properties of pure gelatin or CS hydrogels become a big obstacle that limits their use as scaffolds, especially in load-bearing tissues.					
30184788	3	50	theme	moduli	698:703	arg1	characterization					650:665	characterization	650:665	characterization of the compressive and tensile moduli, ultimate tensile stress and strain	650:739	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	6	51	theme	cells	1233:1237	arg1	area					1203:1206	large spreading area	1187:1206	large spreading area of bone mesenchymal stem cells on hydrogel surfaces	1187:1258	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	3	52	theme	single	871:876	arg1	CS					886:887	the single network CS	867:887	the single network CS	867:887	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	2	53	theme	covalent	545:552	arg1	bonds					554:558	covalent bonds	545:558	covalent bonds	545:558	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	3	54	theme	compressive	674:684	arg1	strain					734:739	strain	734:739	strain	734:739	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	3	54	theme	compressive	674:684	arg1	stress					723:728	ultimate tensile stress	706:728	ultimate tensile stress	706:728	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	3	54	theme	compressive	674:684	arg1	moduli					698:703	the compressive and tensile moduli	670:703	the compressive and tensile moduli	670:703	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	6	55	theme	stem	1228:1231	arg1	cells					1233:1237	bone mesenchymal stem cells	1211:1237	bone mesenchymal stem cells	1211:1237	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	6	56	theme	excellent	1118:1126	arg1	biocompatibility					1128:1143	The excellent biocompatibility	1114:1143	The excellent biocompatibility of GelMA-CS hydrogels	1114:1165	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	3	57	theme	mechanical	811:820	arg1	properties					822:831	the mechanical properties	807:831	the mechanical properties of GelMA-CS hydrogels	807:853	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	2	58	theme	gelatin	496:502	arg1	methacryloyl					504:515	gelatin methacryloyl	496:515	gelatin methacryloyl	496:515	This study provided a novel mechanism of forming interpenetrating network (IPN) of gelatin methacryloyl (GelMA) and CS hydrogels by covalent bonds and hydrophobic interactions through photocrosslinking and basification, respectively.					
30184788	6	59	theme	mesenchymal	1216:1226	arg1	cells					1233:1237	bone mesenchymal stem cells	1211:1237	bone mesenchymal stem cells	1211:1237	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
30184788	0	60	with	chitosan	55:62	arg1	properties					120:129	enhanced mechanical properties	100:129	enhanced mechanical properties for tissue engineering	100:152	Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering.					
30184788	3	61	theme	tensile	715:721	arg1	stress					723:728	ultimate tensile stress	706:728	ultimate tensile stress	706:728	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	3	61	theme	tensile	715:721	arg1	moduli					698:703	the compressive and tensile moduli	670:703	the compressive and tensile moduli	670:703	By characterization of the compressive and tensile moduli, ultimate tensile stress and strain, it was found that semi-IPN and IPN structure can greatly enhance the mechanical properties of GelMA-CS hydrogels compared to the single network CS or GelMA.					
30184788	6	62	theme	bone	1211:1214	arg1	cells					1233:1237	bone mesenchymal stem cells	1211:1237	bone mesenchymal stem cells	1211:1237	The excellent biocompatibility of GelMA-CS hydrogels was demonstrated by large spreading area of bone mesenchymal stem cells on hydrogel surfaces when CS concentration was <2% (w/v).					
31449399	1	0	with	nanocarriers	149:160	arg1	drugs					167:171	drugs	167:171	drugs	167:171	Few studies have considered the interaction of nanocarriers with drugs and the implications for their individual efficiency.					
31449399	7	1	theme	obvious	946:952	arg1	change					954:959	no obvious change	943:959	no obvious change	943:959	Heparin induced no obvious change in the protein corona composition; thus, we suggest that heparin itself, through its adsorption on the nanocarrier, was responsible for the change of uptake.					
31449399	1	2	theme	nanocarriers	149:160	arg1	implications					181:192	the implications	177:192	the implications for their individual efficiency	177:224	Few studies have considered the interaction of nanocarriers with drugs and the implications for their individual efficiency.					
31449399	1	2	theme	nanocarriers	149:160	arg1	interaction					134:144	the interaction	130:144	the interaction of nanocarriers with drugs	130:171	Few studies have considered the interaction of nanocarriers with drugs and the implications for their individual efficiency.					
31449399	0	3	theme	Cellular	69:76	arg1	Uptake					78:83	the Cellular Uptake	65:83	the Cellular Uptake of Nanocarriers	65:99	Timing of Heparin Addition to the Biomolecular Corona Influences the Cellular Uptake of Nanocarriers.					
31449399	6	4	theme	primary	872:878	arg1	monocytes					880:888	primary monocytes	872:888	primary monocytes	872:888	We found that HeLa cells, monocytes and macrophages reacted differently to the presence of heparin: the uptake of the precoated nanocarriers decreased for HeLa and primary monocytes, while it increased for macrophages.					
31449399	0	5	theme	Nanocarriers	88:99	arg1	Uptake					78:83	the Cellular Uptake	65:83	the Cellular Uptake of Nanocarriers	65:99	Timing of Heparin Addition to the Biomolecular Corona Influences the Cellular Uptake of Nanocarriers.					
31449399	4	6	theme	cellular	557:564	arg1	uptake					566:571	the cellular uptake	553:571	the cellular uptake extent	553:578	The relation between the timing of the heparin's addition to the nanocarrier and the cellular uptake extent was assessed by flow cytometry.					
31449399	3	7	from	plasma	363:368	arg1	conditions					383:392	different conditions	373:392	different conditions	373:392	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	3	8	theme	precoated	336:344	arg1	nanocarriers					322:333	nanocarriers	322:333	nanocarriers	322:333	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	2	9	theme	common	264:269	arg1	anticoagulant					271:283	a common anticoagulant	262:283	a common anticoagulant	262:283	Here, we demonstrate that heparin, a common anticoagulant, interacts with nanocarriers.					
31449399	2	9	theme	common	264:269	arg1	heparin					253:259	heparin	253:259	heparin	253:259	Here, we demonstrate that heparin, a common anticoagulant, interacts with nanocarriers.					
31449399	3	10	theme	different	373:381	arg1	conditions					383:392	different conditions	373:392	different conditions	373:392	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	6	11	theme	nanocarriers	836:847	arg1	uptake					812:817	the uptake	808:817	the uptake of the precoated nanocarriers	808:847	We found that HeLa cells, monocytes and macrophages reacted differently to the presence of heparin: the uptake of the precoated nanocarriers decreased for HeLa and primary monocytes, while it increased for macrophages.					
31449399	3	12	theme	cancer	415:420	arg1	cells					422:426	cancer cells	415:426	cancer cells	415:426	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	4	13	theme	addition	521:528	arg1	extent					573:578	the cellular uptake extent	553:578	the cellular uptake extent	553:578	The relation between the timing of the heparin's addition to the nanocarrier and the cellular uptake extent was assessed by flow cytometry.					
31449399	4	13	theme	addition	521:528	arg1	timing					497:502	the timing	493:502	the timing of the heparin's addition to the nanocarrier	493:547	The relation between the timing of the heparin's addition to the nanocarrier and the cellular uptake extent was assessed by flow cytometry.					
31449399	3	14	from	blood	465:469	arg1	cells					422:426	cancer cells	415:426	cancer cells	415:426	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	3	14	from	blood	465:469	arg1	cells					448:452	primary cells	440:452	primary cells from human blood	440:469	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	0	15	theme	Addition	18:25	arg1	Timing					0:5	Timing	0:5	Timing of Heparin Addition to the Biomolecular Corona	0:52	Timing of Heparin Addition to the Biomolecular Corona Influences the Cellular Uptake of Nanocarriers.					
31449399	5	16	theme	biomolecular	660:671	arg1	composition					680:690	the biomolecular corona composition	656:690	the biomolecular corona composition	656:690	Through proteomics the effect of heparin on the biomolecular corona composition was determined.					
31449399	4	17	theme	flow	596:599	arg1	cytometry					601:609	flow cytometry	596:609	flow cytometry	596:609	The relation between the timing of the heparin's addition to the nanocarrier and the cellular uptake extent was assessed by flow cytometry.					
31449399	0	18	theme	Heparin	10:16	arg1	Addition					18:25	Heparin Addition	10:25	Heparin Addition	10:25	Timing of Heparin Addition to the Biomolecular Corona Influences the Cellular Uptake of Nanocarriers.					
31449399	5	19	theme	corona	673:678	arg1	composition					680:690	the biomolecular corona composition	656:690	the biomolecular corona composition	656:690	Through proteomics the effect of heparin on the biomolecular corona composition was determined.					
31449399	6	20	theme	precoated	826:834	arg1	nanocarriers					836:847	the precoated nanocarriers	822:847	the precoated nanocarriers	822:847	We found that HeLa cells, monocytes and macrophages reacted differently to the presence of heparin: the uptake of the precoated nanocarriers decreased for HeLa and primary monocytes, while it increased for macrophages.					
31449399	1	21	theme	Few	102:104	arg1	studies					106:112	Few studies	102:112	Few studies	102:112	Few studies have considered the interaction of nanocarriers with drugs and the implications for their individual efficiency.					
31449399	7	22	from	adsorption	1046:1055	arg1	nanocarrier					1064:1074	the nanocarrier	1060:1074	the nanocarrier	1060:1074	Heparin induced no obvious change in the protein corona composition; thus, we suggest that heparin itself, through its adsorption on the nanocarrier, was responsible for the change of uptake.					
31449399	3	23	with	precoated	336:344	arg1	plasma					363:368	plasma	363:368	plasma	363:368	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	3	23	with	precoated	336:344	arg1	heparin					351:357	heparin	351:357	heparin	351:357	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	1	24	theme	individual	204:213	arg1	efficiency					215:224	their individual efficiency	198:224	their individual efficiency	198:224	Few studies have considered the interaction of nanocarriers with drugs and the implications for their individual efficiency.					
31449399	3	25	from	heparin	351:357	arg1	conditions					383:392	different conditions	373:392	different conditions	373:392	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	7	26	theme	corona	976:981	arg1	composition					983:993	the protein corona composition	964:993	the protein corona composition	964:993	Heparin induced no obvious change in the protein corona composition; thus, we suggest that heparin itself, through its adsorption on the nanocarrier, was responsible for the change of uptake.					
31449399	5	27	from	effect	635:640	arg1	composition					680:690	the biomolecular corona composition	656:690	the biomolecular corona composition	656:690	Through proteomics the effect of heparin on the biomolecular corona composition was determined.					
31449399	0	28	theme	Biomolecular	34:45	arg1	Corona					47:52	the Biomolecular Corona	30:52	the Biomolecular Corona	30:52	Timing of Heparin Addition to the Biomolecular Corona Influences the Cellular Uptake of Nanocarriers.					
31449399	5	29	theme	heparin	645:651	arg1	effect					635:640	the effect	631:640	the effect of heparin on the biomolecular corona composition	631:690	Through proteomics the effect of heparin on the biomolecular corona composition was determined.					
31449399	6	30	theme	HeLa	722:725	arg1	macrophages					748:758	macrophages	748:758	macrophages	748:758	We found that HeLa cells, monocytes and macrophages reacted differently to the presence of heparin: the uptake of the precoated nanocarriers decreased for HeLa and primary monocytes, while it increased for macrophages.					
31449399	6	30	theme	HeLa	722:725	arg1	monocytes					734:742	monocytes	734:742	monocytes	734:742	We found that HeLa cells, monocytes and macrophages reacted differently to the presence of heparin: the uptake of the precoated nanocarriers decreased for HeLa and primary monocytes, while it increased for macrophages.					
31449399	6	30	theme	HeLa	722:725	arg1	cells					727:731	HeLa cells	722:731	HeLa cells	722:731	We found that HeLa cells, monocytes and macrophages reacted differently to the presence of heparin: the uptake of the precoated nanocarriers decreased for HeLa and primary monocytes, while it increased for macrophages.					
31449399	7	31	theme	protein	968:974	arg1	composition					983:993	the protein corona composition	964:993	the protein corona composition	964:993	Heparin induced no obvious change in the protein corona composition; thus, we suggest that heparin itself, through its adsorption on the nanocarrier, was responsible for the change of uptake.					
31449399	6	32	theme	heparin	799:805	arg1	presence					787:794	the presence	783:794	the presence of heparin	783:805	We found that HeLa cells, monocytes and macrophages reacted differently to the presence of heparin: the uptake of the precoated nanocarriers decreased for HeLa and primary monocytes, while it increased for macrophages.					
31449399	3	33	theme	human	459:463	arg1	blood					465:469	human blood	459:469	human blood	459:469	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	4	34	theme	uptake	566:571	arg1	extent					573:578	the cellular uptake extent	553:578	the cellular uptake extent	553:578	The relation between the timing of the heparin's addition to the nanocarrier and the cellular uptake extent was assessed by flow cytometry.					
31449399	3	35	theme	primary	440:446	arg1	cells					448:452	primary cells	440:452	primary cells from human blood	440:469	Hence, nanocarriers, precoated with heparin and plasma in different conditions, were incubated with cancer cells, as well as primary cells from human blood.					
31449399	7	36	theme	uptake	1111:1116	arg1	change					1101:1106	the change	1097:1106	the change of uptake	1097:1116	Heparin induced no obvious change in the protein corona composition; thus, we suggest that heparin itself, through its adsorption on the nanocarrier, was responsible for the change of uptake.					
29393589	3	0	theme	serum	361:365	arg1	molecules					437:445	the test molecules	428:445	the test molecules	428:445	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	0	theme	serum	361:365	arg1	BSA					376:378	BSA	376:378	BSA	376:378	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	0	theme	serum	361:365	arg1	albumin					367:373	Bovine serum albumin	354:373	Bovine serum albumin (BSA)	354:379	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	0	theme	serum	361:365	arg1	oligonucleotide					395:409	activated oligonucleotide	385:409	activated oligonucleotide	385:409	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	4	1	theme	BSA	509:511	arg1	PEGylation					525:534	BSA solid-phase PEGylation	509:534	BSA solid-phase PEGylation	509:534	BSA solid-phase PEGylation is performed on Q Sepharose HP.					
29393589	5	2	theme	linear	654:659	arg1	experiments					684:694	linear gradient elution (LGE) experiments	654:694	linear gradient elution (LGE) experiments	654:694	Distribution coefficient of BSA and PEG-BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model.					
29393589	9	3	theme	monolithic	1115:1124	arg1	column					1126:1131	monolithic CIM QA disk monolithic column	1092:1131	monolithic CIM QA disk monolithic column	1092:1131	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	10	4	theme	PEG	1210:1212	arg1	composition					1222:1232	PEG reagent composition	1210:1232	PEG reagent composition	1210:1232	Selectivity of over 2000 is obtained by proper adjustment of PEG reagent composition.					
29393589	2	5	dep	possibility	196:206	arg1	induce					211:216	induce	211:216	induce	211:216	In this work possibility to induce spontaneous desorption of adsorbed macromolecules, once being PEGylated, through adjustment of the reagent composition is investigated.					
29393589	12	6	theme	reaction	1436:1443	arg1	conditions					1445:1454	the reaction conditions	1432:1454	the reaction conditions	1432:1454	Both experiments demonstrates that reaction-mediated desorption of macromolecules is possible when the reaction conditions are properly tuned.					
29393589	5	7	theme	gradient	661:668	arg1	experiments					684:694	linear gradient elution (LGE) experiments	654:694	linear gradient elution (LGE) experiments	654:694	Distribution coefficient of BSA and PEG-BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model.					
29393589	8	8	theme	low	938:940	arg1	selectivity					942:952	a rather low selectivity	929:952	a rather low selectivity	929:952	However, due to a rather low selectivity, also desorption of BSA occurred at high elution volume.					
29393589	7	9	theme	PEG	866:868	arg1	desorption					852:861	Spontaneous desorption	840:861	Spontaneous desorption of PEG - BSA	840:874	Spontaneous desorption of PEG - BSA is detected with no presence of BSA.					
29393589	11	10	theme	High	1235:1238	arg1	selectivity					1240:1250	High selectivity	1235:1250	High selectivity	1235:1250	High selectivity enables spontaneous desorption of PEG-9T without any desorption of activated 9T.					
29393589	3	11	theme	activated	385:393	arg1	albumin					367:373	Bovine serum albumin	354:373	Bovine serum albumin (BSA)	354:379	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	11	theme	activated	385:393	arg1	molecules					437:445	the test molecules	428:445	the test molecules	428:445	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	11	theme	activated	385:393	arg1	9T					412:413	9T	412:413	9T	412:413	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	11	theme	activated	385:393	arg1	oligonucleotide					395:409	activated oligonucleotide	385:409	activated oligonucleotide	385:409	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	7	12	theme	BSA	908:910	arg1	presence					896:903	no presence	893:903	no presence of BSA	893:910	Spontaneous desorption of PEG - BSA is detected with no presence of BSA.					
29393589	5	13	theme	elution	670:676	arg1	experiments					684:694	linear gradient elution (LGE) experiments	654:694	linear gradient elution (LGE) experiments	654:694	Distribution coefficient of BSA and PEG-BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model.					
29393589	2	14	theme	composition	325:335	arg1	adjustment					299:308	adjustment	299:308	adjustment of the reagent composition	299:335	In this work possibility to induce spontaneous desorption of adsorbed macromolecules, once being PEGylated, through adjustment of the reagent composition is investigated.					
29393589	9	15	theme	similar	1013:1019	arg1	procedure					1021:1029	A similar procedure	1011:1029	A similar procedure	1011:1029	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	8	16	theme	high	990:993	arg1	volume					1003:1008	high elution volume	990:1008	high elution volume	990:1008	However, due to a rather low selectivity, also desorption of BSA occurred at high elution volume.					
29393589	2	17	theme	reagent	317:323	arg1	composition					325:335	the reagent composition	313:335	the reagent composition	313:335	In this work possibility to induce spontaneous desorption of adsorbed macromolecules, once being PEGylated, through adjustment of the reagent composition is investigated.					
29393589	7	18	theme	Spontaneous	840:850	arg1	desorption					852:861	Spontaneous desorption	840:861	Spontaneous desorption of PEG - BSA	840:874	Spontaneous desorption of PEG - BSA is detected with no presence of BSA.					
29393589	5	19	theme	NaCl	629:632	arg1	function					617:624	a function	615:624	a function of NaCl	615:632	Distribution coefficient of BSA and PEG-BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model.					
29393589	12	20	theme	macromolecules	1400:1413	arg1	desorption					1386:1395	reaction-mediated desorption	1368:1395	reaction-mediated desorption of macromolecules	1368:1413	Both experiments demonstrates that reaction-mediated desorption of macromolecules is possible when the reaction conditions are properly tuned.					
29393589	3	21	theme	20 kDa	451:456	arg1	PEG					475:477	20 kDa linear activated PEG	451:477	20 kDa linear activated PEG	451:477	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	11	22	theme	9T	1329:1330	arg1	desorption					1305:1314	any desorption	1301:1314	any desorption of activated 9T	1301:1330	High selectivity enables spontaneous desorption of PEG-9T without any desorption of activated 9T.					
29393589	0	23	theme	Reaction-Mediated	0:16	arg1	Desorption					18:27	Reaction-Mediated Desorption	0:27	Reaction-Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation.	0:110	Reaction-Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation.					
29393589	3	24	theme	linear	458:463	arg1	PEG					475:477	20 kDa linear activated PEG	451:477	20 kDa linear activated PEG	451:477	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	5	25	theme	BSA	596:598	arg1	coefficient					581:591	Distribution coefficient	568:591	Distribution coefficient of BSA and PEG-BSA as a function of NaCl	568:632	Distribution coefficient of BSA and PEG-BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model.					
29393589	3	26	theme	Bovine	354:359	arg1	molecules					437:445	the test molecules	428:445	the test molecules	428:445	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	26	theme	Bovine	354:359	arg1	BSA					376:378	BSA	376:378	BSA	376:378	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	26	theme	Bovine	354:359	arg1	albumin					367:373	Bovine serum albumin	354:373	Bovine serum albumin (BSA)	354:379	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	26	theme	Bovine	354:359	arg1	oligonucleotide					395:409	activated oligonucleotide	385:409	activated oligonucleotide	385:409	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	1	27	with	reaction	131:138	arg1	separation					145:154	separation	145:154	separation	145:154	Combining chemical reaction with separation offers several advantages.					
29393589	11	28	theme	spontaneous	1260:1270	arg1	desorption					1272:1281	spontaneous desorption	1260:1281	spontaneous desorption of PEG-9T	1260:1291	High selectivity enables spontaneous desorption of PEG-9T without any desorption of activated 9T.					
29393589	8	29	theme	BSA	974:976	arg1	desorption					960:969	desorption	960:969	desorption of BSA	960:976	However, due to a rather low selectivity, also desorption of BSA occurred at high elution volume.					
29393589	1	30	theme	Combining	112:120	arg1	reaction					131:138	Combining chemical reaction	112:138	Combining chemical reaction with separation	112:154	Combining chemical reaction with separation offers several advantages.					
29393589	9	31	theme	activated	1046:1054	arg1	oligonucleotide					1059:1073	activated 9T oligonucleotide	1046:1073	activated 9T oligonucleotide	1046:1073	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	9	31	theme	activated	1046:1054	arg1	time					1081:1084	this time	1076:1084	this time using monolithic CIM QA disk monolithic column for adsorption	1076:1146	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	0	32	theme	Macromolecules	32:45	arg1	Desorption					18:27	Reaction-Mediated Desorption	0:27	Reaction-Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation.	0:110	Reaction-Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation.					
29393589	12	33	theme	reaction-mediated	1368:1384	arg1	desorption					1386:1395	reaction-mediated desorption	1368:1395	reaction-mediated desorption of macromolecules	1368:1413	Both experiments demonstrates that reaction-mediated desorption of macromolecules is possible when the reaction conditions are properly tuned.					
29393589	5	34	theme	Yamamoto	700:707	arg1	model					709:713	Yamamoto model	700:713	Yamamoto model	700:713	Distribution coefficient of BSA and PEG-BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model.					
29393589	5	35	theme	PEG-BSA	604:610	arg1	coefficient					581:591	Distribution coefficient	568:591	Distribution coefficient of BSA and PEG-BSA as a function of NaCl	568:632	Distribution coefficient of BSA and PEG-BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model.					
29393589	3	36	used	used	482:485	arg2	PEG					475:477	20 kDa linear activated PEG	451:477	20 kDa linear activated PEG	451:477	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	9	37	theme	9T	1056:1057	arg1	oligonucleotide					1059:1073	activated 9T oligonucleotide	1046:1073	activated 9T oligonucleotide	1046:1073	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	9	37	theme	9T	1056:1057	arg1	time					1081:1084	this time	1076:1084	this time using monolithic CIM QA disk monolithic column for adsorption	1076:1146	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	9	38	theme	QA	1107:1108	arg1	column					1126:1131	monolithic CIM QA disk monolithic column	1092:1131	monolithic CIM QA disk monolithic column	1092:1131	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	0	39	theme	Novel	48:52	arg1	Phenomenon					54:63	Novel Phenomenon	48:63	Reaction-Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation.	0:110	Reaction-Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation.					
29393589	10	40	theme	reagent	1214:1220	arg1	composition					1222:1232	PEG reagent composition	1210:1232	PEG reagent composition	1210:1232	Selectivity of over 2000 is obtained by proper adjustment of PEG reagent composition.					
29393589	3	41	theme	activated	465:473	arg1	PEG					475:477	20 kDa linear activated PEG	451:477	20 kDa linear activated PEG	451:477	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	42	theme	test	432:435	arg1	molecules					437:445	the test molecules	428:445	the test molecules	428:445	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	42	theme	test	432:435	arg1	albumin					367:373	Bovine serum albumin	354:373	Bovine serum albumin (BSA)	354:379	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	42	theme	test	432:435	arg1	oligonucleotide					395:409	activated oligonucleotide	385:409	activated oligonucleotide	385:409	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	11	43	theme	PEG-9T	1286:1291	arg1	desorption					1272:1281	spontaneous desorption	1260:1281	spontaneous desorption of PEG-9T	1260:1291	High selectivity enables spontaneous desorption of PEG-9T without any desorption of activated 9T.					
29393589	5	44	theme	LGE	679:681	arg1	experiments					684:694	linear gradient elution (LGE) experiments	654:694	linear gradient elution (LGE) experiments	654:694	Distribution coefficient of BSA and PEG-BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model.					
29393589	11	45	theme	activated	1319:1327	arg1	9T					1329:1330	activated 9T	1319:1330	activated 9T	1319:1330	High selectivity enables spontaneous desorption of PEG-9T without any desorption of activated 9T.					
29393589	4	46	theme	Sepharose	554:562	arg1	HP					564:565	Q Sepharose HP	552:565	Q Sepharose HP	552:565	BSA solid-phase PEGylation is performed on Q Sepharose HP.					
29393589	3	47	used	used	420:423	arg2	oligonucleotide					395:409	activated oligonucleotide	385:409	activated oligonucleotide	385:409	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	47	used	used	420:423	arg2	molecules					437:445	the test molecules	428:445	the test molecules	428:445	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	47	used	used	420:423	arg2	BSA					376:378	BSA	376:378	BSA	376:378	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	47	used	used	420:423	arg2	9T					412:413	9T	412:413	9T	412:413	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	3	47	used	used	420:423	arg2	albumin					367:373	Bovine serum albumin	354:373	Bovine serum albumin (BSA)	354:379	Bovine serum albumin (BSA) and activated oligonucleotide, 9T, are used as the test molecules and 20 kDa linear activated PEG is used for their PEGylation.					
29393589	2	48	theme	adsorbed	244:251	arg1	macromolecules					253:266	adsorbed macromolecules	244:266	adsorbed macromolecules	244:266	In this work possibility to induce spontaneous desorption of adsorbed macromolecules, once being PEGylated, through adjustment of the reagent composition is investigated.					
29393589	2	49	theme	macromolecules	253:266	arg1	desorption					230:239	spontaneous desorption	218:239	spontaneous desorption of adsorbed macromolecules	218:266	In this work possibility to induce spontaneous desorption of adsorbed macromolecules, once being PEGylated, through adjustment of the reagent composition is investigated.					
29393589	5	50	theme	Distribution	568:579	arg1	coefficient					581:591	Distribution coefficient	568:591	Distribution coefficient of BSA and PEG-BSA as a function of NaCl	568:632	Distribution coefficient of BSA and PEG-BSA as a function of NaCl is determined using linear gradient elution (LGE) experiments and Yamamoto model.					
29393589	8	51	theme	elution	995:1001	arg1	volume					1003:1008	high elution volume	990:1008	high elution volume	990:1008	However, due to a rather low selectivity, also desorption of BSA occurred at high elution volume.					
29393589	10	52	theme	proper	1189:1194	arg1	adjustment					1196:1205	proper adjustment	1189:1205	proper adjustment of PEG reagent composition	1189:1232	Selectivity of over 2000 is obtained by proper adjustment of PEG reagent composition.					
29393589	4	53	theme	Q	552:552	arg1	HP					564:565	Q Sepharose HP	552:565	Q Sepharose HP	552:565	BSA solid-phase PEGylation is performed on Q Sepharose HP.					
29393589	9	54	theme	monolithic	1092:1101	arg1	column					1126:1131	monolithic CIM QA disk monolithic column	1092:1131	monolithic CIM QA disk monolithic column	1092:1131	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	1	55	theme	chemical	122:129	arg1	reaction					131:138	Combining chemical reaction	112:138	Combining chemical reaction with separation	112:154	Combining chemical reaction with separation offers several advantages.					
29393589	10	56	theme	composition	1222:1232	arg1	adjustment					1196:1205	proper adjustment	1189:1205	proper adjustment of PEG reagent composition	1189:1232	Selectivity of over 2000 is obtained by proper adjustment of PEG reagent composition.					
29393589	9	57	theme	CIM	1103:1105	arg1	column					1126:1131	monolithic CIM QA disk monolithic column	1092:1131	monolithic CIM QA disk monolithic column	1092:1131	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	1	58	theme	several	163:169	arg1	advantages					171:180	several advantages	163:180	several advantages	163:180	Combining chemical reaction with separation offers several advantages.					
29393589	2	59	theme	spontaneous	218:228	arg1	desorption					230:239	spontaneous desorption	218:239	spontaneous desorption of adsorbed macromolecules	218:266	In this work possibility to induce spontaneous desorption of adsorbed macromolecules, once being PEGylated, through adjustment of the reagent composition is investigated.					
29393589	0	60	dep	Desorption	18:27	arg1	Phenomenon					54:63	Novel Phenomenon	48:63	Reaction-Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation.	0:110	Reaction-Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation.					
29393589	6	61	theme	distribution	733:744	arg1	coefficient					746:756	the distribution coefficient	729:756	the distribution coefficient	729:756	According to the distribution coefficient the selectivity between BSA and PEG - BSA of around 15 is adjusted by using NaCl.					
29393589	0	62	theme	Simultaneous	74:85	arg1	Reaction					87:94	Simultaneous Reaction	74:94	Simultaneous Reaction	74:94	Reaction-Mediated Desorption of Macromolecules: Novel Phenomenon Enabling Simultaneous Reaction and Separation.					
29393589	4	63	theme	solid-phase	513:523	arg1	PEGylation					525:534	BSA solid-phase PEGylation	509:534	BSA solid-phase PEGylation	509:534	BSA solid-phase PEGylation is performed on Q Sepharose HP.					
29393589	9	64	theme	disk	1110:1113	arg1	column					1126:1131	monolithic CIM QA disk monolithic column	1092:1131	monolithic CIM QA disk monolithic column	1092:1131	A similar procedure is applied for activated 9T oligonucleotide, this time using monolithic CIM QA disk monolithic column for adsorption.					
29393589	7	65	theme	BSA	872:874	arg1	desorption					852:861	Spontaneous desorption	840:861	Spontaneous desorption of PEG - BSA	840:874	Spontaneous desorption of PEG - BSA is detected with no presence of BSA.					
30127608	0	0	theme	bone	110:113	arg1	regeneration					115:126	guided bone regeneration	103:126	guided bone regeneration	103:126	Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration.					
30127608	2	1	theme	silver	554:559	arg1	CaP					572:574	silver ion-loaded CaP	554:574	silver ion-loaded CaP	554:574	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	2	2	theme	biomimetic	389:398	arg1	membrane					471:478	biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	389:478	biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	389:478	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	6	3	with	membranes	1241:1249	arg1	properties					1308:1317	strong antimicrobial properties	1287:1317	strong antimicrobial properties	1287:1317	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	6	3	with	membranes	1241:1249	arg1	biocompatibility					1266:1281	excellent biocompatibility	1256:1281	excellent biocompatibility	1256:1281	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	0	4	theme	guided	103:108	arg1	regeneration					115:126	guided bone regeneration	103:126	guided bone regeneration	103:126	Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration.					
30127608	3	5	dep	RESULTS	658:664	arg1	revealed					711:718	revealed	711:718	revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes	711:892	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	6	6	contain	have	1319:1322	arg2	potential					1330:1338	great potential	1324:1338	great potential	1324:1338	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	6	6	contain	have	1319:1322	arg1	membranes					1241:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes	1190:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties	1190:1317	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	2	7	theme	electrospinning	591:605	arg1	method					607:612	a one-step electrospinning method	580:612	a one-step electrospinning method	580:612	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	5	8	theme	MTT	1079:1081	arg1	assay					1083:1087	MTT assay	1079:1087	MTT assay	1079:1087	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	1	9	theme	native	281:286	arg1	matrix					302:307	the native extracellular matrix	277:307	the native extracellular matrix	277:307	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	2	10	theme	one-step	582:589	arg1	method					607:612	a one-step electrospinning method	580:612	a one-step electrospinning method	580:612	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	6	11	theme	great	1324:1328	arg1	potential					1330:1338	great potential	1324:1338	great potential	1324:1338	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	5	12	theme	assay	1083:1087	arg1	results					1050:1056	The results	1046:1056	The results of cell adhesion and MTT assay	1046:1087	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	1	13	theme	extracellular	288:300	arg1	matrix					302:307	the native extracellular matrix	277:307	the native extracellular matrix	277:307	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	5	14	with	cytocompatible	1132:1145	arg1	cells					1172:1176	bone marrow stromal cells	1152:1176	bone marrow stromal cells	1152:1176	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	6	15	theme	Ag-CaP/CS	1221:1229	arg1	membranes					1241:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes	1190:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties	1190:1317	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	6	16	theme	strong	1287:1292	arg1	properties					1308:1317	strong antimicrobial properties	1287:1317	strong antimicrobial properties	1287:1317	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	2	17	theme	ion-loaded	421:430	arg1	Ag-CaP/CS					460:468	Ag-CaP/CS	460:468	Ag-CaP/CS	460:468	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	2	17	theme	ion-loaded	421:430	arg1	phosphate/chitosan					440:457	silver ion-loaded calcium phosphate/chitosan	414:457	biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	389:478	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	1	18	theme	guided	181:186	arg1	membrane					206:213	a guided bone regeneration membrane	179:213	a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties	179:348	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	1	18	theme	guided	181:186	arg1	similar					223:229	similar	223:229	similar	223:229	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	2	19	with	MATERIALS	351:359	arg1	ability					499:505	antibacterial ability	485:505	antibacterial ability	485:505	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	3	20	theme	CaP	822:824	arg1	addition					810:817	the addition	806:817	the addition of CaP	806:824	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	4	21	theme	Ag+	967:969	arg1	release					956:962	a sustainable release	942:962	a sustainable release of Ag+, which in turn inhibited the adhesion and growth of Staphylococcus mutans	942:1043	Importantly, the Ag-CaP/CS membranes exhibited a sustainable release of Ag+, which in turn inhibited the adhesion and growth of Staphylococcus mutans.					
30127608	6	22	theme	nanofiber	1231:1239	arg1	membranes					1241:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes	1190:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties	1190:1317	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	1	23	theme	bone	188:191	arg1	membrane					206:213	a guided bone regeneration membrane	179:213	a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties	179:348	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	1	23	theme	bone	188:191	arg1	similar					223:229	similar	223:229	similar	223:229	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	0	24	theme	silver	12:17	arg1	calcium					30:36	Electrospun silver ion-loaded calcium	0:36	Electrospun silver ion-loaded calcium	0:36	Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration.					
30127608	1	25	theme	regeneration	193:204	arg1	membrane					206:213	a guided bone regeneration membrane	179:213	a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties	179:348	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	1	25	theme	regeneration	193:204	arg1	similar					223:229	similar	223:229	similar	223:229	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	0	26	theme	Electrospun	0:10	arg1	calcium					30:36	Electrospun silver ion-loaded calcium	0:36	Electrospun silver ion-loaded calcium	0:36	Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration.					
30127608	6	27	theme	bone	1362:1365	arg1	regeneration					1367:1378	guided bone regeneration	1355:1378	guided bone regeneration	1355:1378	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	5	28	theme	cell	1061:1064	arg1	adhesion					1066:1073	cell adhesion	1061:1073	cell adhesion	1061:1073	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	2	29	theme	silver	414:419	arg1	Ag-CaP/CS					460:468	Ag-CaP/CS	460:468	Ag-CaP/CS	460:468	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	2	29	theme	silver	414:419	arg1	phosphate/chitosan					440:457	silver ion-loaded calcium phosphate/chitosan	414:457	biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	389:478	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	2	30	theme	antibacterial	485:497	arg1	ability					499:505	antibacterial ability	485:505	antibacterial ability	485:505	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	6	31	theme	electrospinning	1205:1219	arg1	membranes					1241:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes	1190:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties	1190:1317	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	6	32	dep	CONCLUSION	1179:1188	arg1	have					1319:1322	have	1319:1322	have great potential to be used for guided bone regeneration	1319:1378	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	5	33	theme	adhesion	1066:1073	arg1	results					1050:1056	The results	1046:1056	The results of cell adhesion and MTT assay	1046:1087	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	5	34	theme	stromal	1164:1170	arg1	cells					1172:1176	bone marrow stromal cells	1152:1176	bone marrow stromal cells	1152:1176	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	2	35	theme	membrane	471:478	arg1	type					381:384	A novel type	373:384	A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	373:478	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	0	36	theme	ion-loaded	19:28	arg1	calcium					30:36	Electrospun silver ion-loaded calcium	0:36	Electrospun silver ion-loaded calcium	0:36	Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration.					
30127608	1	37	theme	sufficient	314:323	arg1	properties					339:348	sufficient antibacterial properties	314:348	sufficient antibacterial properties	314:348	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	4	38	theme	mutans	1038:1043	arg1	growth					1013:1018	growth	1013:1018	growth	1013:1018	Importantly, the Ag-CaP/CS membranes exhibited a sustainable release of Ag+, which in turn inhibited the adhesion and growth of Staphylococcus mutans.					
30127608	4	38	theme	mutans	1038:1043	arg1	adhesion					1000:1007	adhesion	1000:1007	adhesion	1000:1007	Importantly, the Ag-CaP/CS membranes exhibited a sustainable release of Ag+, which in turn inhibited the adhesion and growth of Staphylococcus mutans.					
30127608	2	39	dep	MATERIALS	351:359	arg1	type					381:384	A novel type	373:384	A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	373:478	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	2	40	theme	bioactive	404:412	arg1	membrane					471:478	biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	389:478	biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	389:478	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	0	41	theme	antibacterial	57:69	arg1	membranes					89:97	antibacterial composite fibrous membranes	57:97	antibacterial composite fibrous membranes for guided bone regeneration	57:126	Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration.					
30127608	1	42	theme	antibacterial	325:337	arg1	properties					339:348	sufficient antibacterial properties	314:348	sufficient antibacterial properties	314:348	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	4	43	dep	adhesion	1000:1007	arg1	the					996:998	the	996:998	the	996:998	Importantly, the Ag-CaP/CS membranes exhibited a sustainable release of Ag+, which in turn inhibited the adhesion and growth of Staphylococcus mutans.					
30127608	2	44	with	METHODS	365:371	arg1	ability					499:505	antibacterial ability	485:505	antibacterial ability	485:505	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	4	45	theme	sustainable	944:954	arg1	release					956:962	a sustainable release	942:962	a sustainable release of Ag+, which in turn inhibited the adhesion and growth of Staphylococcus mutans	942:1043	Importantly, the Ag-CaP/CS membranes exhibited a sustainable release of Ag+, which in turn inhibited the adhesion and growth of Staphylococcus mutans.					
30127608	2	46	theme	phosphate/chitosan	440:457	arg1	membrane					471:478	biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	389:478	biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	389:478	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	3	47	theme	fabricated	729:738	arg1	membranes					748:756	the fabricated fibrous membranes	725:756	the fabricated fibrous membranes	725:756	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	3	48	theme	physicochemical	684:698	arg1	properties					700:709	the physicochemical properties	680:709	the physicochemical properties	680:709	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	6	49	theme	fabricated	1194:1203	arg1	membranes					1241:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes	1190:1249	The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties	1190:1317	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	0	50	theme	fibrous	81:87	arg1	membranes					89:97	antibacterial composite fibrous membranes	57:97	antibacterial composite fibrous membranes for guided bone regeneration	57:126	Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration.					
30127608	3	51	theme	fibrous	740:746	arg1	membranes					748:756	the fabricated fibrous membranes	725:756	the fabricated fibrous membranes	725:756	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	5	52	theme	bone	1152:1155	arg1	cells					1172:1176	bone marrow stromal cells	1152:1176	bone marrow stromal cells	1152:1176	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	6	53	theme	excellent	1256:1264	arg1	biocompatibility					1266:1281	excellent biocompatibility	1256:1281	excellent biocompatibility	1256:1281	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	6	54	theme	antimicrobial	1294:1306	arg1	properties					1308:1317	strong antimicrobial properties	1287:1317	strong antimicrobial properties	1287:1317	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
30127608	0	55	theme	composite	71:79	arg1	membranes					89:97	antibacterial composite fibrous membranes	57:97	antibacterial composite fibrous membranes for guided bone regeneration	57:126	Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration.					
30127608	3	56	theme	membranes	884:892	arg1	ability					869:875	the mineralization ability	850:875	the mineralization ability of the membranes	850:892	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	3	57	theme	matrix	785:790	arg1	structure					792:800	the extracellular matrix structure	767:800	the extracellular matrix structure	767:800	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	5	58	theme	marrow	1157:1162	arg1	cells					1172:1176	bone marrow stromal cells	1152:1176	bone marrow stromal cells	1152:1176	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	2	59	theme	novel	375:379	arg1	type					381:384	A novel type	373:384	A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	373:478	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	4	60	theme	Ag-CaP/CS	912:920	arg1	membranes					922:930	the Ag-CaP/CS membranes	908:930	the Ag-CaP/CS membranes	908:930	Importantly, the Ag-CaP/CS membranes exhibited a sustainable release of Ag+, which in turn inhibited the adhesion and growth of Staphylococcus mutans.					
30127608	3	61	theme	mineralization	854:867	arg1	ability					869:875	the mineralization ability	850:875	the mineralization ability of the membranes	850:892	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	1	62	theme	bone	234:237	arg1	components					239:248	bone components	234:248	bone components	234:248	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	5	63	theme	Ag-CaP/CS	1107:1115	arg1	membranes					1117:1125	the Ag-CaP/CS membranes	1103:1125	the Ag-CaP/CS membranes	1103:1125	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	5	63	theme	Ag-CaP/CS	1107:1115	arg1	cytocompatible					1132:1145	cytocompatible	1132:1145	cytocompatible	1132:1145	The results of cell adhesion and MTT assay revealed that the Ag-CaP/CS membranes were cytocompatible with bone marrow stromal cells.					
30127608	1	64	theme	study	157:161	arg1	purpose					141:147	The purpose	137:147	The purpose of this study	137:161	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	2	65	theme	CaP	572:574	arg1	incorporation					537:549	incorporation	537:549	incorporation of silver ion-loaded CaP via a one-step electrospinning method	537:612	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	3	66	theme	extracellular	771:783	arg1	structure					792:800	the extracellular matrix structure	767:800	the extracellular matrix structure	767:800	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	3	67	theme	properties	700:709	arg1	Evaluation					666:675	Evaluation	666:675	Evaluation of the physicochemical properties	666:709	RESULTS Evaluation of the physicochemical properties revealed that the fabricated fibrous membranes mimicked the extracellular matrix structure and the addition of CaP significantly increased the mineralization ability of the membranes.					
30127608	1	68	dep	PURPOSE	129:135	arg1	is					163:164	is	163:164	is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties	163:348	PURPOSE The purpose of this study is to construct a guided bone regeneration membrane that is similar to bone components and structurally resembles the native extracellular matrix with sufficient antibacterial properties.					
30127608	2	69	theme	calcium	432:438	arg1	Ag-CaP/CS					460:468	Ag-CaP/CS	460:468	Ag-CaP/CS	460:468	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	2	69	theme	calcium	432:438	arg1	phosphate/chitosan					440:457	silver ion-loaded calcium phosphate/chitosan	414:457	biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane	389:478	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	2	70	theme	ion-loaded	561:570	arg1	CaP					572:574	silver ion-loaded CaP	554:574	silver ion-loaded CaP	554:574	MATERIALS AND METHODS A novel type of biomimetic and bioactive silver ion-loaded calcium phosphate/chitosan (Ag-CaP/CS) membrane with antibacterial ability was successfully developed by incorporation of silver ion-loaded CaP via a one-step electrospinning method and subsequently crosslinked with vanillin.					
30127608	6	71	theme	guided	1355:1360	arg1	regeneration					1367:1378	guided bone regeneration	1355:1378	guided bone regeneration	1355:1378	CONCLUSION The fabricated electrospinning Ag-CaP/CS nanofiber membranes with excellent biocompatibility and strong antimicrobial properties have great potential to be used for guided bone regeneration.					
29408201	6	0	with	surface	841:847	arg1	particles					864:872	spherical particles	854:872	spherical particles on the laccase/HP treated chitosan	854:907	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	4	1	theme	X-ray	557:561	arg1	diffraction					563:573	X-ray diffraction	557:573	X-ray diffraction (XRD)	557:579	X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan.					
29408201	4	1	theme	X-ray	557:561	arg1	XRD					576:578	XRD	576:578	XRD	576:578	X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan.					
29408201	10	2	with	composite	1392:1400	arg1	functionalities					1414:1428	double functionalities	1407:1428	double functionalities (i.e., antioxidant and hydrophobic)	1407:1464	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	7	3	theme	thermal	1062:1068	arg1	stability					1070:1078	the thermal stability	1058:1078	the thermal stability of copolymer	1058:1091	The laccase/HP treatment of chitosan improved the thermal stability of copolymer.					
29408201	10	4	theme	packaging	1500:1508	arg1	materials					1510:1518	food packaging materials or coating agents	1495:1536	materials	1510:1518	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	4	theme	packaging	1500:1508	arg1	type					1384:1387	This new type	1375:1387	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic)	1375:1464	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	5	theme	new	1380:1382	arg1	materials					1510:1518	food packaging materials or coating agents	1495:1536	materials	1510:1518	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	5	theme	new	1380:1382	arg1	agents					1531:1536	food packaging materials or coating agents	1495:1536	agents	1531:1536	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	5	theme	new	1380:1382	arg1	type					1384:1387	This new type	1375:1387	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic)	1375:1464	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	4	6	dep	showed	581:586	arg1	compared					662:669	compared	662:669	showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan	581:686	X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan.					
29408201	7	7	theme	chitosan	1040:1047	arg1	treatment					1027:1035	The laccase/HP treatment	1012:1035	The laccase/HP treatment of chitosan	1012:1047	The laccase/HP treatment of chitosan improved the thermal stability of copolymer.					
29408201	2	8	theme	thermal	393:399	arg1	stability					401:409	thermal stability	393:409	thermal stability	393:409	The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength.					
29408201	4	9	theme	crystallinity	616:628	arg1	degree					606:611	the degree	602:611	the degree of crystallinity for laccase/HP treated chitosan	602:660	X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan.					
29408201	8	10	theme	pure	1228:1231	arg1	chitosan					1233:1240	pure chitosan	1228:1240	pure chitosan	1228:1240	More significantly, the HP functionalized chitosan showed greatly improved ABTS+ and DPPH radicals scavenging capacity, compared with pure chitosan.					
29408201	6	11	theme	probe	988:992	arg1	microscopy					994:1003	scanning probe microscopy	979:1003	scanning probe microscopy (SPM)	979:1009	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	6	11	theme	probe	988:992	arg1	SPM					1006:1008	SPM	1006:1008	SPM	1006:1008	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	5	12	theme	laccase/HP-treated	715:732	arg1	chitosan					734:741	laccase/HP-treated chitosan	715:741	laccase/HP-treated chitosan	715:741	The grafted-HP content in laccase/HP-treated chitosan first increased and then declined with increase of the initial HP/chitosan ratio.					
29408201	6	13	theme	treated	892:898	arg1	chitosan					900:907	the laccase/HP treated chitosan	877:907	the laccase/HP treated chitosan	877:907	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	2	14	theme	chemical	333:340	arg1	structure					342:350	chemical structure	333:350	chemical structure	333:350	The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength.					
29408201	4	15	theme	treated	645:651	arg1	chitosan					653:660	laccase/HP treated chitosan	634:660	laccase/HP treated chitosan	634:660	X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan.					
29408201	0	16	theme	copolymer	120:128	arg1	properties					106:115	antioxidant and hydrophobic properties	78:115	antioxidant and hydrophobic properties of copolymer	78:128	Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol enhances antioxidant and hydrophobic properties of copolymer.					
29408201	6	17	theme	scanning	979:986	arg1	microscopy					994:1003	scanning probe microscopy	979:1003	scanning probe microscopy (SPM)	979:1009	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	6	17	theme	scanning	979:986	arg1	SPM					1006:1008	SPM	1006:1008	SPM	1006:1008	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	8	18	theme	HP	1118:1119	arg1	chitosan					1136:1143	the HP functionalized chitosan	1114:1143	the HP functionalized chitosan	1114:1143	More significantly, the HP functionalized chitosan showed greatly improved ABTS+ and DPPH radicals scavenging capacity, compared with pure chitosan.					
29408201	6	19	theme	laccase/HP	881:890	arg1	chitosan					900:907	the laccase/HP treated chitosan	877:907	the laccase/HP treated chitosan	877:907	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	10	20	theme	double	1407:1412	arg1	functionalities					1414:1428	double functionalities	1407:1428	double functionalities (i.e., antioxidant and hydrophobic)	1407:1464	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	4	21	theme	laccase/HP	634:643	arg1	chitosan					653:660	laccase/HP treated chitosan	634:660	laccase/HP treated chitosan	634:660	X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan.					
29408201	5	22	theme	HP/chitosan	806:816	arg1	ratio					818:822	the initial HP/chitosan ratio	794:822	the initial HP/chitosan ratio	794:822	The grafted-HP content in laccase/HP-treated chitosan first increased and then declined with increase of the initial HP/chitosan ratio.					
29408201	9	23	theme	chitosan	1296:1303	arg1	property					1262:1269	The hydrophobicity property	1243:1269	The hydrophobicity property of the HP functionalized chitosan	1243:1303	The hydrophobicity property of the HP functionalized chitosan also significantly increased although its tensile strength decreased.					
29408201	1	24	dep	method	144:149	arg1	functionalize					154:166	functionalize	154:166	to functionalize chitosan with 4-hexyloxyphenol (HP) under homogeneous reaction conditions	151:240	An effective method to functionalize chitosan with 4-hexyloxyphenol (HP) under homogeneous reaction conditions was developed using laccase as the catalyst.					
29408201	3	25	theme	Solid-state	479:489	arg1	NMR					495:497	Solid-state 13C NMR	479:497	Solid-state 13C NMR spectrum	479:506	Solid-state 13C NMR spectrum confirmed the incorporation of HP onto chitosan.					
29408201	3	26	theme	NMR	495:497	arg1	spectrum					499:506	Solid-state 13C NMR spectrum	479:506	Solid-state 13C NMR spectrum	479:506	Solid-state 13C NMR spectrum confirmed the incorporation of HP onto chitosan.					
29408201	5	27	theme	ratio	818:822	arg1	increase					782:789	increase	782:789	increase of the initial HP/chitosan ratio	782:822	The grafted-HP content in laccase/HP-treated chitosan first increased and then declined with increase of the initial HP/chitosan ratio.					
29408201	10	28	theme	composite	1392:1400	arg1	materials					1510:1518	food packaging materials or coating agents	1495:1536	materials	1510:1518	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	28	theme	composite	1392:1400	arg1	agents					1531:1536	food packaging materials or coating agents	1495:1536	agents	1531:1536	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	28	theme	composite	1392:1400	arg1	type					1384:1387	This new type	1375:1387	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic)	1375:1464	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	2	29	theme	hydrophobic	434:444	arg1	properties					446:455	hydrophobic properties	434:455	hydrophobic properties	434:455	The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength.					
29408201	8	30	theme	improved	1160:1167	arg1	ABTS+					1169:1173	greatly improved ABTS+	1152:1173	greatly improved ABTS+	1152:1173	More significantly, the HP functionalized chitosan showed greatly improved ABTS+ and DPPH radicals scavenging capacity, compared with pure chitosan.					
29408201	7	31	theme	laccase/HP	1016:1025	arg1	treatment					1027:1035	The laccase/HP treatment	1012:1035	The laccase/HP treatment of chitosan	1012:1047	The laccase/HP treatment of chitosan improved the thermal stability of copolymer.					
29408201	1	32	theme	homogeneous	210:220	arg1	conditions					231:240	homogeneous reaction conditions	210:240	homogeneous reaction conditions	210:240	An effective method to functionalize chitosan with 4-hexyloxyphenol (HP) under homogeneous reaction conditions was developed using laccase as the catalyst.					
29408201	0	33	theme	Laccase-mediated	0:15	arg1	functionalization					17:33	Laccase-mediated functionalization	0:33	Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol	0:67	Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol enhances antioxidant and hydrophobic properties of copolymer.					
29408201	5	34	theme	grafted-HP	693:702	arg1	content					704:710	The grafted-HP content	689:710	The grafted-HP content in laccase/HP-treated chitosan	689:741	The grafted-HP content in laccase/HP-treated chitosan first increased and then declined with increase of the initial HP/chitosan ratio.					
29408201	6	35	theme	spherical	854:862	arg1	particles					864:872	spherical particles	854:872	spherical particles on the laccase/HP treated chitosan	854:907	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	2	36	theme	grafted-HP	353:362	arg1	content					364:370	grafted-HP content	353:370	grafted-HP content	353:370	The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength.					
29408201	3	37	theme	13C	491:493	arg1	NMR					495:497	Solid-state 13C NMR	479:497	Solid-state 13C NMR spectrum	479:506	Solid-state 13C NMR spectrum confirmed the incorporation of HP onto chitosan.					
29408201	0	38	theme	chitosan	38:45	arg1	functionalization					17:33	Laccase-mediated functionalization	0:33	Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol	0:67	Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol enhances antioxidant and hydrophobic properties of copolymer.					
29408201	10	39	theme	coating	1523:1529	arg1	agents					1531:1536	food packaging materials or coating agents	1495:1536	agents	1531:1536	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	39	theme	coating	1523:1529	arg1	type					1384:1387	This new type	1375:1387	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic)	1375:1464	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	40	dep	antioxidant	1437:1447	arg1	i.e.					1431:1434	i.e.	1431:1434	i.e.	1431:1434	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	7	41	theme	copolymer	1083:1091	arg1	stability					1070:1078	the thermal stability	1058:1078	the thermal stability of copolymer	1058:1091	The laccase/HP treatment of chitosan improved the thermal stability of copolymer.					
29408201	1	42	theme	effective	134:142	arg1	method					144:149	An effective method	131:149	An effective method to functionalize chitosan with 4-hexyloxyphenol (HP) under homogeneous reaction conditions	131:240	An effective method to functionalize chitosan with 4-hexyloxyphenol (HP) under homogeneous reaction conditions was developed using laccase as the catalyst.					
29408201	4	43	from	decrease	590:597	arg1	degree					606:611	the degree	602:611	the degree of crystallinity for laccase/HP treated chitosan	602:660	X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan.					
29408201	9	44	theme	tensile	1347:1353	arg1	strength					1355:1362	its tensile strength	1343:1362	its tensile strength	1343:1362	The hydrophobicity property of the HP functionalized chitosan also significantly increased although its tensile strength decreased.					
29408201	1	45	theme	reaction	222:229	arg1	conditions					231:240	homogeneous reaction conditions	210:240	homogeneous reaction conditions	210:240	An effective method to functionalize chitosan with 4-hexyloxyphenol (HP) under homogeneous reaction conditions was developed using laccase as the catalyst.					
29408201	2	46	theme	tensile	461:467	arg1	strength					469:476	tensile strength	461:476	tensile strength	461:476	The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength.					
29408201	8	47	theme	DPPH	1179:1182	arg1	radicals					1184:1191	DPPH radicals	1179:1191	DPPH radicals	1179:1191	More significantly, the HP functionalized chitosan showed greatly improved ABTS+ and DPPH radicals scavenging capacity, compared with pure chitosan.					
29408201	6	48	theme	electron	948:955	arg1	ESEM					969:972	ESEM	969:972	ESEM	969:972	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	6	48	theme	electron	948:955	arg1	microscopy					957:966	environmental scanning electron microscopy	925:966	environmental scanning electron microscopy (ESEM)	925:973	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	6	49	from	particles	864:872	arg1	chitosan					900:907	the laccase/HP treated chitosan	877:907	the laccase/HP treated chitosan	877:907	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	0	50	theme	antioxidant	78:88	arg1	properties					106:115	antioxidant and hydrophobic properties	78:115	antioxidant and hydrophobic properties of copolymer	78:128	Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol enhances antioxidant and hydrophobic properties of copolymer.					
29408201	9	51	theme	functionalized	1281:1294	arg1	chitosan					1296:1303	the HP functionalized chitosan	1274:1303	the HP functionalized chitosan	1274:1303	The hydrophobicity property of the HP functionalized chitosan also significantly increased although its tensile strength decreased.					
29408201	6	52	theme	scanning	939:946	arg1	ESEM					969:972	ESEM	969:972	ESEM	969:972	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	6	52	theme	scanning	939:946	arg1	microscopy					957:966	environmental scanning electron microscopy	925:966	environmental scanning electron microscopy (ESEM)	925:973	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	6	53	theme	heterogeneous	827:839	arg1	surface					841:847	A heterogeneous surface	825:847	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan	825:907	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	8	54	dep	ABTS+	1169:1173	arg1	capacity					1204:1211	scavenging capacity	1193:1211	scavenging capacity	1193:1211	More significantly, the HP functionalized chitosan showed greatly improved ABTS+ and DPPH radicals scavenging capacity, compared with pure chitosan.					
29408201	5	55	from	content	704:710	arg1	chitosan					734:741	laccase/HP-treated chitosan	715:741	laccase/HP-treated chitosan	715:741	The grafted-HP content in laccase/HP-treated chitosan first increased and then declined with increase of the initial HP/chitosan ratio.					
29408201	8	56	theme	scavenging	1193:1202	arg1	capacity					1204:1211	scavenging capacity	1193:1211	scavenging capacity	1193:1211	More significantly, the HP functionalized chitosan showed greatly improved ABTS+ and DPPH radicals scavenging capacity, compared with pure chitosan.					
29408201	6	57	theme	environmental	925:937	arg1	ESEM					969:972	ESEM	969:972	ESEM	969:972	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	6	57	theme	environmental	925:937	arg1	microscopy					957:966	environmental scanning electron microscopy	925:966	environmental scanning electron microscopy (ESEM)	925:973	A heterogeneous surface with spherical particles on the laccase/HP treated chitosan was observed by environmental scanning electron microscopy (ESEM) and scanning probe microscopy (SPM).					
29408201	0	58	with	functionalization	17:33	arg1	4-hexyloxyphenol					52:67	4-hexyloxyphenol	52:67	4-hexyloxyphenol	52:67	Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol enhances antioxidant and hydrophobic properties of copolymer.					
29408201	8	59	theme	functionalized	1121:1134	arg1	chitosan					1136:1143	the HP functionalized chitosan	1114:1143	the HP functionalized chitosan	1114:1143	More significantly, the HP functionalized chitosan showed greatly improved ABTS+ and DPPH radicals scavenging capacity, compared with pure chitosan.					
29408201	10	60	theme	food	1495:1498	arg1	materials					1510:1518	food packaging materials or coating agents	1495:1536	materials	1510:1518	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	60	theme	food	1495:1498	arg1	type					1384:1387	This new type	1375:1387	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic)	1375:1464	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	9	61	theme	HP	1278:1279	arg1	chitosan					1296:1303	the HP functionalized chitosan	1274:1303	the HP functionalized chitosan	1274:1303	The hydrophobicity property of the HP functionalized chitosan also significantly increased although its tensile strength decreased.					
29408201	2	62	theme	surface	373:379	arg1	morphology					381:390	surface morphology	373:390	surface morphology	373:390	The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength.					
29408201	2	63	theme	resulting	291:299	arg1	copolymer					301:309	The resulting copolymer	287:309	The resulting copolymer	287:309	The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength.					
29408201	3	64	theme	HP	539:540	arg1	incorporation					522:534	the incorporation	518:534	the incorporation of HP onto chitosan	518:554	Solid-state 13C NMR spectrum confirmed the incorporation of HP onto chitosan.					
29408201	0	65	theme	hydrophobic	94:104	arg1	properties					106:115	antioxidant and hydrophobic properties	78:115	antioxidant and hydrophobic properties of copolymer	78:128	Laccase-mediated functionalization of chitosan with 4-hexyloxyphenol enhances antioxidant and hydrophobic properties of copolymer.					
29408201	9	66	theme	hydrophobicity	1247:1260	arg1	property					1262:1269	The hydrophobicity property	1243:1269	The hydrophobicity property of the HP functionalized chitosan	1243:1303	The hydrophobicity property of the HP functionalized chitosan also significantly increased although its tensile strength decreased.					
29408201	2	67	theme	antioxidant	412:422	arg1	capacity					424:431	antioxidant capacity	412:431	antioxidant capacity	412:431	The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength.					
29408201	10	68	used	used	1487:1490	arg2	type					1384:1387	This new type	1375:1387	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic)	1375:1464	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	68	used	used	1487:1490	arg2	agents					1531:1536	food packaging materials or coating agents	1495:1536	agents	1531:1536	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	10	68	used	used	1487:1490	arg2	materials					1510:1518	food packaging materials or coating agents	1495:1536	materials	1510:1518	This new type of composite with double functionalities (i.e., antioxidant and hydrophobic) could potentially be used as food packaging materials or coating agents.					
29408201	4	69	theme	pure	674:677	arg1	chitosan					679:686	pure chitosan	674:686	pure chitosan	674:686	X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan.					
29408201	5	70	theme	initial	798:804	arg1	ratio					818:822	the initial HP/chitosan ratio	794:822	the initial HP/chitosan ratio	794:822	The grafted-HP content in laccase/HP-treated chitosan first increased and then declined with increase of the initial HP/chitosan ratio.					
31056297	6	0	theme	scaffolds	980:988	arg1	extracts					934:941	aqueous extracts	926:941	aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days	926:1005	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	4	1	theme	microscopic	612:622	arg1	micrographs					624:634	scanning electron microscopic micrographs	594:634	scanning electron microscopic micrographs	594:634	Cell growth and adhesion were compared using fluorescein isothiocyanate-phalloidin staining and scanning electron microscopic micrographs.					
31056297	13	2	theme	calcium	1988:1994	arg1	ions					2011:2014	more calcium and phosphorus ions	1983:2014	more calcium and phosphorus ions	1983:2014	3D-printed scaffold aqueous extract contained more calcium and phosphorus ions than the Alg-Gel scaffold.					
31056297	5	3	theme	complete	692:699	arg1	medium					709:714	the complete culture medium	688:714	the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds	688:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	11	4	theme	negative	1629:1636	arg1	group					1646:1650	the negative control group	1625:1650	the negative control group	1625:1650	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	6	5	theme	3D-printed	961:970	arg1	scaffolds					980:988	the Alg-Gel or 3D-printed Alg-Gel scaffolds	946:988	the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7	946:994	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	12	6	theme	genes	1908:1912	arg1	expression					1871:1880	The expression	1867:1880	The expression of mineralization-related genes	1867:1912	The expression of mineralization-related genes was also up-regulated.					
31056297	9	7	dep	RESULTS	1333:1339	arg1	adhered					1367:1373	adhered	1367:1373	adhered	1367:1373	RESULTS More cells were grown and adhered to the 3D-printed Alg-Gel scaffolds than the Alg-Gel scaffolds.					
31056297	9	7	dep	RESULTS	1333:1339	arg1	grown					1357:1361	grown	1357:1361	grown	1357:1361	RESULTS More cells were grown and adhered to the 3D-printed Alg-Gel scaffolds than the Alg-Gel scaffolds.					
31056297	13	8	theme	3D-printed	1937:1946	arg1	extract					1965:1971	3D-printed scaffold aqueous extract	1937:1971	3D-printed scaffold aqueous extract	1937:1971	3D-printed scaffold aqueous extract contained more calcium and phosphorus ions than the Alg-Gel scaffold.					
31056297	7	9	theme	genes	1180:1184	arg1	expression					1143:1152	The messenger RNA and protein expression	1113:1152	The messenger RNA and protein expression of mineralization-related genes	1113:1184	The messenger RNA and protein expression of mineralization-related genes were detected with real-time polymerase chain reaction and Western blotting.					
31056297	10	10	theme	3D-printed	1463:1472	arg1	scaffolds					1474:1482	3D-printed scaffolds	1463:1482	3D-printed scaffolds	1463:1482	The aqueous extracts of 3D-printed scaffolds can promote cell proliferation, and compared with Alg-Gel scaffolds, the extracts of 3D-printed scaffolds were more effective.					
31056297	6	11	theme	Alg-Gel	950:956	arg1	scaffolds					980:988	the Alg-Gel or 3D-printed Alg-Gel scaffolds	946:988	the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7	946:994	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	2	12	from	effect	353:358	arg1	hDPSCs					410:415	hDPSCs	410:415	hDPSCs	410:415	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	2	12	from	effect	353:358	arg1	cells					403:407	human dental pulp stem cells	380:407	human dental pulp stem cells (hDPSCs)	380:416	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	2	13	theme	pulp	393:396	arg1	hDPSCs					410:415	hDPSCs	410:415	hDPSCs	410:415	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	2	13	theme	pulp	393:396	arg1	cells					403:407	human dental pulp stem cells	380:407	human dental pulp stem cells (hDPSCs)	380:416	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	8	14	theme	extract	1312:1318	arg1	composition					1320:1330	the material extract composition	1299:1330	the material extract composition	1299:1330	Elemental analysis was used to test the material extract composition.					
31056297	13	15	theme	aqueous	1957:1963	arg1	extract					1965:1971	3D-printed scaffold aqueous extract	1937:1971	3D-printed scaffold aqueous extract	1937:1971	3D-printed scaffold aqueous extract contained more calcium and phosphorus ions than the Alg-Gel scaffold.					
31056297	11	16	theme	3D-printed	1653:1662	arg1	scaffold					1672:1679	3D-printed Alg-Gel scaffold	1653:1679	3D-printed Alg-Gel scaffold	1653:1679	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	8	17	theme	Elemental	1263:1271	arg1	analysis					1273:1280	Elemental analysis	1263:1280	Elemental analysis	1263:1280	Elemental analysis was used to test the material extract composition.					
31056297	5	18	theme	aqueous	727:733	arg1	extracts					735:742	aqueous extracts	727:742	aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds	727:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	7	19	theme	protein	1135:1141	arg1	expression					1143:1152	The messenger RNA and protein expression	1113:1152	The messenger RNA and protein expression of mineralization-related genes	1113:1184	The messenger RNA and protein expression of mineralization-related genes were detected with real-time polymerase chain reaction and Western blotting.					
31056297	10	20	theme	cell	1496:1499	arg1	proliferation					1501:1513	cell proliferation	1496:1513	cell proliferation	1496:1513	The aqueous extracts of 3D-printed scaffolds can promote cell proliferation, and compared with Alg-Gel scaffolds, the extracts of 3D-printed scaffolds were more effective.					
31056297	5	21	theme	Alg-Gel	751:757	arg1	scaffolds					781:789	the Alg-Gel or 3D-printed Alg-Gel scaffolds	747:789	the Alg-Gel or 3D-printed Alg-Gel scaffolds	747:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	5	22	theme	3D-printed	762:771	arg1	scaffolds					781:789	the Alg-Gel or 3D-printed Alg-Gel scaffolds	747:789	the Alg-Gel or 3D-printed Alg-Gel scaffolds	747:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	6	23	theme	14 days	999:1005	arg1	extracts					934:941	aqueous extracts	926:941	aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days	926:1005	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	14	24	dep	cell	2226:2229	arg1	proliferation					2231:2243	proliferation	2231:2243	proliferation	2231:2243	CONCLUSIONS These findings suggest that compared with the Alg-Gel scaffold, 3D-printed Alg-Gel is more suitable for the growth of hDPSCs, and the scaffold extracts can better promote cell proliferation and differentiation.					
31056297	1	25	from	research	256:263	arg1	application					272:282	its application	268:282	its application in dental tissues	268:300	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold has been applied in tissue engineering, but the research on its application in dental tissues regeneration is still lacking.					
31056297	7	26	theme	mineralization-related	1157:1178	arg1	genes					1180:1184	mineralization-related genes	1157:1184	mineralization-related genes	1157:1184	The messenger RNA and protein expression of mineralization-related genes were detected with real-time polymerase chain reaction and Western blotting.					
31056297	8	27	used	used	1286:1289	arg2	analysis					1273:1280	Elemental analysis	1263:1280	Elemental analysis	1263:1280	Elemental analysis was used to test the material extract composition.					
31056297	7	28	theme	real-time	1205:1213	arg1	reaction					1232:1239	real-time polymerase chain reaction	1205:1239	real-time polymerase chain reaction	1205:1239	The messenger RNA and protein expression of mineralization-related genes were detected with real-time polymerase chain reaction and Western blotting.					
31056297	11	29	theme	control	1638:1644	arg1	group					1646:1650	the negative control group	1625:1650	the negative control group	1625:1650	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	1	30	theme	INTRODUCTION	150:161	arg1	scaffold					199:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold	150:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold	150:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold has been applied in tissue engineering, but the research on its application in dental tissues regeneration is still lacking.					
31056297	6	31	theme	S	1067:1067	arg1	staining					1069:1076	alizarin red S staining	1054:1076	alizarin red S staining	1054:1076	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	5	32	theme	Cell	811:814	arg1	assay					831:835	Cell Counting Kit-8 assay	811:835	Cell Counting Kit-8 assay	811:835	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	5	33	from	proliferation	652:664	arg1	hDPSCs					669:674	hDPSCs	669:674	hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds	669:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	6	34	theme	alizarin	1054:1061	arg1	staining					1069:1076	alizarin red S staining	1054:1076	alizarin red S staining	1054:1076	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	4	35	theme	Cell	498:501	arg1	growth					503:508	Cell growth	498:508	Cell growth	498:508	Cell growth and adhesion were compared using fluorescein isothiocyanate-phalloidin staining and scanning electron microscopic micrographs.					
31056297	10	36	theme	Alg-Gel	1534:1540	arg1	scaffolds					1542:1550	Alg-Gel scaffolds	1534:1550	Alg-Gel scaffolds	1534:1550	The aqueous extracts of 3D-printed scaffolds can promote cell proliferation, and compared with Alg-Gel scaffolds, the extracts of 3D-printed scaffolds were more effective.					
31056297	5	37	theme	Kit-8	825:829	arg1	assay					831:835	Cell Counting Kit-8 assay	811:835	Cell Counting Kit-8 assay	811:835	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	1	38	theme	Alg-Gel	190:196	arg1	scaffold					199:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold	150:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold	150:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold has been applied in tissue engineering, but the research on its application in dental tissues regeneration is still lacking.					
31056297	0	39	from	Effects	0:6	arg1	Differentiation					101:115	Differentiation	101:115	Differentiation	101:115	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	0	39	from	Effects	0:6	arg1	Proliferation					83:95	Proliferation	83:95	Proliferation	83:95	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	5	40	theme	scaffolds	781:789	arg1	extracts					735:742	aqueous extracts	727:742	aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds	727:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	6	41	theme	cells	1035:1039	arg1	differentiation					1016:1030	the differentiation	1012:1030	the differentiation of cells	1012:1039	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	13	42	theme	Alg-Gel	2025:2031	arg1	scaffold					2033:2040	the Alg-Gel scaffold	2021:2040	the Alg-Gel scaffold	2021:2040	3D-printed scaffold aqueous extract contained more calcium and phosphorus ions than the Alg-Gel scaffold.					
31056297	0	43	theme	Dental	126:131	arg1	Cells					143:147	Human Dental Pulp Stem Cells	120:147	Human Dental Pulp Stem Cells	120:147	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	9	44	theme	Alg-Gel	1393:1399	arg1	scaffolds					1401:1409	the 3D-printed Alg-Gel scaffolds	1378:1409	the 3D-printed Alg-Gel scaffolds than the Alg-Gel scaffolds	1378:1436	RESULTS More cells were grown and adhered to the 3D-printed Alg-Gel scaffolds than the Alg-Gel scaffolds.					
31056297	0	45	theme	Hydrogel	54:61	arg1	Extract					72:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	14	46	theme	hDPSCs	2173:2178	arg1	growth					2163:2168	the growth	2159:2168	the growth of hDPSCs	2159:2178	CONCLUSIONS These findings suggest that compared with the Alg-Gel scaffold, 3D-printed Alg-Gel is more suitable for the growth of hDPSCs, and the scaffold extracts can better promote cell proliferation and differentiation.					
31056297	3	47	theme	METHODS	419:425	arg1	hDPSCs					427:432	METHODS hDPSCs	419:432	METHODS hDPSCs	419:432	METHODS hDPSCs were cultured in both Alg-Gel and 3D-printed Alg-Gel scaffolds.					
31056297	1	48	theme	dental	287:292	arg1	tissues					294:300	dental tissues	287:300	dental tissues	287:300	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold has been applied in tissue engineering, but the research on its application in dental tissues regeneration is still lacking.					
31056297	4	49	theme	isothiocyanate-phalloidin	555:579	arg1	staining					581:588	fluorescein isothiocyanate-phalloidin staining	543:588	fluorescein isothiocyanate-phalloidin staining	543:588	Cell growth and adhesion were compared using fluorescein isothiocyanate-phalloidin staining and scanning electron microscopic micrographs.					
31056297	13	50	theme	phosphorus	2000:2009	arg1	ions					2011:2014	more calcium and phosphorus ions	1983:2014	more calcium and phosphorus ions	1983:2014	3D-printed scaffold aqueous extract contained more calcium and phosphorus ions than the Alg-Gel scaffold.					
31056297	3	51	theme	Alg-Gel	456:462	arg1	scaffolds					487:495	both Alg-Gel and 3D-printed Alg-Gel scaffolds	451:495	both Alg-Gel and 3D-printed Alg-Gel scaffolds	451:495	METHODS hDPSCs were cultured in both Alg-Gel and 3D-printed Alg-Gel scaffolds.					
31056297	0	52	theme	3-dimensional	11:23	arg1	Extract					72:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	9	53	theme	Alg-Gel	1420:1426	arg1	scaffolds					1428:1436	the Alg-Gel scaffolds	1416:1436	the Alg-Gel scaffolds	1416:1436	RESULTS More cells were grown and adhered to the 3D-printed Alg-Gel scaffolds than the Alg-Gel scaffolds.					
31056297	6	54	theme	phosphatase	1091:1101	arg1	staining					1103:1110	alkaline phosphatase staining	1082:1110	alkaline phosphatase staining	1082:1110	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	10	55	theme	3D-printed	1569:1578	arg1	scaffolds					1580:1588	3D-printed scaffolds	1569:1588	3D-printed scaffolds	1569:1588	The aqueous extracts of 3D-printed scaffolds can promote cell proliferation, and compared with Alg-Gel scaffolds, the extracts of 3D-printed scaffolds were more effective.					
31056297	0	56	theme	Alginate/Gelatin	37:52	arg1	Extract					72:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	7	57	theme	chain	1226:1230	arg1	reaction					1232:1239	real-time polymerase chain reaction	1205:1239	real-time polymerase chain reaction	1205:1239	The messenger RNA and protein expression of mineralization-related genes were detected with real-time polymerase chain reaction and Western blotting.					
31056297	11	58	theme	nodules	1820:1826	arg1	staining					1857:1864	the alkaline phosphatase staining	1832:1864	the alkaline phosphatase staining	1832:1864	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	11	58	theme	nodules	1820:1826	arg1	formation					1797:1805	the enhanced formation	1784:1805	the enhanced formation of bone-like nodules	1784:1826	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	13	59	contain	contained	1973:1981	arg1	extract					1965:1971	3D-printed scaffold aqueous extract	1937:1971	3D-printed scaffold aqueous extract	1937:1971	3D-printed scaffold aqueous extract contained more calcium and phosphorus ions than the Alg-Gel scaffold.					
31056297	13	59	contain	contained	1973:1981	arg2	ions					2011:2014	more calcium and phosphorus ions	1983:2014	more calcium and phosphorus ions	1983:2014	3D-printed scaffold aqueous extract contained more calcium and phosphorus ions than the Alg-Gel scaffold.					
31056297	11	60	theme	scaffold	1693:1700	arg1	extracts					1710:1717	Alg-Gel scaffold aqueous extracts	1685:1717	Alg-Gel scaffold aqueous extracts	1685:1717	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	5	61	theme	cytometry	846:854	arg1	analysis					856:863	flow cytometry analysis	841:863	flow cytometry analysis	841:863	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	6	62	theme	mineralization	893:906	arg1	medium					908:913	the mineralization medium	889:913	the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days	889:1005	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	5	63	contain	containing	716:725	arg2	extracts					735:742	aqueous extracts	727:742	aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds	727:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	5	63	contain	containing	716:725	arg1	medium					709:714	the complete culture medium	688:714	the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds	688:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	3	64	theme	3D-printed	468:477	arg1	scaffolds					487:495	both Alg-Gel and 3D-printed Alg-Gel scaffolds	451:495	both Alg-Gel and 3D-printed Alg-Gel scaffolds	451:495	METHODS hDPSCs were cultured in both Alg-Gel and 3D-printed Alg-Gel scaffolds.					
31056297	1	65	theme	hydrogel	180:187	arg1	scaffold					199:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold	150:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold	150:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold has been applied in tissue engineering, but the research on its application in dental tissues regeneration is still lacking.					
31056297	11	66	theme	phosphatase	1845:1855	arg1	staining					1857:1864	the alkaline phosphatase staining	1832:1864	the alkaline phosphatase staining	1832:1864	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	10	67	theme	aqueous	1443:1449	arg1	extracts					1451:1458	The aqueous extracts	1439:1458	The aqueous extracts of 3D-printed scaffolds	1439:1482	The aqueous extracts of 3D-printed scaffolds can promote cell proliferation, and compared with Alg-Gel scaffolds, the extracts of 3D-printed scaffolds were more effective.					
31056297	2	68	theme	human	380:384	arg1	hDPSCs					410:415	hDPSCs	410:415	hDPSCs	410:415	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	2	68	theme	human	380:384	arg1	cells					403:407	human dental pulp stem cells	380:407	human dental pulp stem cells (hDPSCs)	380:416	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	0	69	theme	Extract	72:78	arg1	Effects					0:6	Effects	0:6	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells	0:147	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	14	70	theme	Alg-Gel	2101:2107	arg1	scaffold					2109:2116	the Alg-Gel scaffold	2097:2116	the Alg-Gel scaffold	2097:2116	CONCLUSIONS These findings suggest that compared with the Alg-Gel scaffold, 3D-printed Alg-Gel is more suitable for the growth of hDPSCs, and the scaffold extracts can better promote cell proliferation and differentiation.					
31056297	13	71	theme	more	1983:1986	arg1	ions					2011:2014	more calcium and phosphorus ions	1983:2014	more calcium and phosphorus ions	1983:2014	3D-printed scaffold aqueous extract contained more calcium and phosphorus ions than the Alg-Gel scaffold.					
31056297	2	72	theme	scaffold	368:375	arg1	effect					353:358	the effect	349:358	the effect of this scaffold on human dental pulp stem cells (hDPSCs)	349:416	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	6	73	theme	Alg-Gel	972:978	arg1	scaffolds					980:988	the Alg-Gel or 3D-printed Alg-Gel scaffolds	946:988	the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7	946:994	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	4	74	theme	electron	603:610	arg1	micrographs					624:634	scanning electron microscopic micrographs	594:634	scanning electron microscopic micrographs	594:634	Cell growth and adhesion were compared using fluorescein isothiocyanate-phalloidin staining and scanning electron microscopic micrographs.					
31056297	5	75	theme	culture	701:707	arg1	medium					709:714	the complete culture medium	688:714	the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds	688:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	10	76	theme	scaffolds	1474:1482	arg1	extracts					1451:1458	The aqueous extracts	1439:1458	The aqueous extracts of 3D-printed scaffolds	1439:1482	The aqueous extracts of 3D-printed scaffolds can promote cell proliferation, and compared with Alg-Gel scaffolds, the extracts of 3D-printed scaffolds were more effective.					
31056297	13	77	theme	scaffold	1948:1955	arg1	extract					1965:1971	3D-printed scaffold aqueous extract	1937:1971	3D-printed scaffold aqueous extract	1937:1971	3D-printed scaffold aqueous extract contained more calcium and phosphorus ions than the Alg-Gel scaffold.					
31056297	0	78	theme	Scaffold	63:70	arg1	Extract					72:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	8	79	theme	material	1303:1310	arg1	composition					1320:1330	the material extract composition	1299:1330	the material extract composition	1299:1330	Elemental analysis was used to test the material extract composition.					
31056297	11	80	theme	enhanced	1788:1795	arg1	formation					1797:1805	the enhanced formation	1784:1805	the enhanced formation of bone-like nodules	1784:1826	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	11	81	theme	Alg-Gel	1664:1670	arg1	scaffold					1672:1679	3D-printed Alg-Gel scaffold	1653:1679	3D-printed Alg-Gel scaffold	1653:1679	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	12	82	theme	mineralization-related	1885:1906	arg1	genes					1908:1912	mineralization-related genes	1885:1912	mineralization-related genes	1885:1912	The expression of mineralization-related genes was also up-regulated.					
31056297	7	83	theme	messenger	1117:1125	arg1	RNA					1127:1129	messenger RNA	1117:1129	messenger RNA	1117:1129	The messenger RNA and protein expression of mineralization-related genes were detected with real-time polymerase chain reaction and Western blotting.					
31056297	1	84	theme	tissue	228:233	arg1	engineering					235:245	tissue engineering	228:245	tissue engineering	228:245	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold has been applied in tissue engineering, but the research on its application in dental tissues regeneration is still lacking.					
31056297	5	85	theme	Alg-Gel	773:779	arg1	scaffolds					781:789	the Alg-Gel or 3D-printed Alg-Gel scaffolds	747:789	the Alg-Gel or 3D-printed Alg-Gel scaffolds	747:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	0	86	theme	Cells	143:147	arg1	Differentiation					101:115	Differentiation	101:115	Differentiation	101:115	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	0	86	theme	Cells	143:147	arg1	Proliferation					83:95	Proliferation	83:95	Proliferation	83:95	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	1	87	from	application	272:282	arg1	tissues					294:300	dental tissues	287:300	dental tissues	287:300	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold has been applied in tissue engineering, but the research on its application in dental tissues regeneration is still lacking.					
31056297	6	88	contain	containing	915:924	arg2	extracts					934:941	aqueous extracts	926:941	aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days	926:1005	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	6	88	contain	containing	915:924	arg1	medium					908:913	the mineralization medium	889:913	the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days	889:1005	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	9	89	theme	More	1341:1344	arg1	cells					1346:1350	More cells	1341:1350	More cells	1341:1350	RESULTS More cells were grown and adhered to the 3D-printed Alg-Gel scaffolds than the Alg-Gel scaffolds.					
31056297	1	90	theme	Alginate/gelatin	163:178	arg1	scaffold					199:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold	150:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold	150:206	INTRODUCTION Alginate/gelatin hydrogel (Alg-Gel) scaffold has been applied in tissue engineering, but the research on its application in dental tissues regeneration is still lacking.					
31056297	6	91	theme	red	1063:1065	arg1	staining					1069:1076	alizarin red S staining	1054:1076	alizarin red S staining	1054:1076	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	5	92	theme	Counting	816:823	arg1	assay					831:835	Cell Counting Kit-8 assay	811:835	Cell Counting Kit-8 assay	811:835	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	5	93	from	Changes	637:643	arg1	proliferation					652:664	the proliferation	648:664	the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds	648:789	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	7	94	theme	RNA	1127:1129	arg1	expression					1143:1152	The messenger RNA and protein expression	1113:1152	The messenger RNA and protein expression of mineralization-related genes	1113:1184	The messenger RNA and protein expression of mineralization-related genes were detected with real-time polymerase chain reaction and Western blotting.					
31056297	0	95	theme	Human	120:124	arg1	Cells					143:147	Human Dental Pulp Stem Cells	120:147	Human Dental Pulp Stem Cells	120:147	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	0	96	theme	Pulp	133:136	arg1	Cells					143:147	Human Dental Pulp Stem Cells	120:147	Human Dental Pulp Stem Cells	120:147	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	9	97	theme	3D-printed	1382:1391	arg1	scaffolds					1401:1409	the 3D-printed Alg-Gel scaffolds	1378:1409	the 3D-printed Alg-Gel scaffolds than the Alg-Gel scaffolds	1378:1436	RESULTS More cells were grown and adhered to the 3D-printed Alg-Gel scaffolds than the Alg-Gel scaffolds.					
31056297	2	98	theme	stem	398:401	arg1	hDPSCs					410:415	hDPSCs	410:415	hDPSCs	410:415	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	2	98	theme	stem	398:401	arg1	cells					403:407	human dental pulp stem cells	380:407	human dental pulp stem cells (hDPSCs)	380:416	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	7	99	theme	Western	1245:1251	arg1	blotting					1253:1260	Western blotting	1245:1260	Western blotting	1245:1260	The messenger RNA and protein expression of mineralization-related genes were detected with real-time polymerase chain reaction and Western blotting.					
31056297	2	100	theme	dental	386:391	arg1	hDPSCs					410:415	hDPSCs	410:415	hDPSCs	410:415	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	2	100	theme	dental	386:391	arg1	cells					403:407	human dental pulp stem cells	380:407	human dental pulp stem cells (hDPSCs)	380:416	We investigated the effect of this scaffold on human dental pulp stem cells (hDPSCs).					
31056297	0	101	theme	Bioprinting	25:35	arg1	Extract					72:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract	11:78	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	11	102	theme	osteogenic/odontoblastic	1728:1751	arg1	differentiation					1753:1767	osteogenic/odontoblastic differentiation	1728:1767	osteogenic/odontoblastic differentiation of hDPSCs	1728:1777	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	4	103	theme	scanning	594:601	arg1	micrographs					624:634	scanning electron microscopic micrographs	594:634	scanning electron microscopic micrographs	594:634	Cell growth and adhesion were compared using fluorescein isothiocyanate-phalloidin staining and scanning electron microscopic micrographs.					
31056297	10	104	theme	scaffolds	1580:1588	arg1	effective					1600:1608	effective	1600:1608	effective	1600:1608	The aqueous extracts of 3D-printed scaffolds can promote cell proliferation, and compared with Alg-Gel scaffolds, the extracts of 3D-printed scaffolds were more effective.					
31056297	10	104	theme	scaffolds	1580:1588	arg1	extracts					1557:1564	the extracts	1553:1564	the extracts of 3D-printed scaffolds	1553:1588	The aqueous extracts of 3D-printed scaffolds can promote cell proliferation, and compared with Alg-Gel scaffolds, the extracts of 3D-printed scaffolds were more effective.					
31056297	7	105	theme	polymerase	1215:1224	arg1	reaction					1232:1239	real-time polymerase chain reaction	1205:1239	real-time polymerase chain reaction	1205:1239	The messenger RNA and protein expression of mineralization-related genes were detected with real-time polymerase chain reaction and Western blotting.					
31056297	6	106	theme	aqueous	926:932	arg1	extracts					934:941	aqueous extracts	926:941	aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days	926:1005	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	6	107	theme	alkaline	1082:1089	arg1	phosphatase					1091:1101	alkaline phosphatase	1082:1101	alkaline phosphatase staining	1082:1110	Cells were cultured in the mineralization medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds for 7 or 14 days, and the differentiation of cells was shown by alizarin red S staining and alkaline phosphatase staining.					
31056297	11	108	theme	bone-like	1810:1818	arg1	nodules					1820:1826	bone-like nodules	1810:1826	bone-like nodules	1810:1826	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	11	109	theme	Alg-Gel	1685:1691	arg1	extracts					1710:1717	Alg-Gel scaffold aqueous extracts	1685:1717	Alg-Gel scaffold aqueous extracts	1685:1717	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	5	110	theme	flow	841:844	arg1	analysis					856:863	flow cytometry analysis	841:863	flow cytometry analysis	841:863	Changes in the proliferation in hDPSCs cultured in the complete culture medium containing aqueous extracts of the Alg-Gel or 3D-printed Alg-Gel scaffolds were examined using Cell Counting Kit-8 assay and flow cytometry analysis.					
31056297	4	111	theme	fluorescein	543:553	arg1	staining					581:588	fluorescein isothiocyanate-phalloidin staining	543:588	fluorescein isothiocyanate-phalloidin staining	543:588	Cell growth and adhesion were compared using fluorescein isothiocyanate-phalloidin staining and scanning electron microscopic micrographs.					
31056297	14	112	theme	3D-printed	2119:2128	arg1	Alg-Gel					2130:2136	3D-printed Alg-Gel	2119:2136	3D-printed Alg-Gel	2119:2136	CONCLUSIONS These findings suggest that compared with the Alg-Gel scaffold, 3D-printed Alg-Gel is more suitable for the growth of hDPSCs, and the scaffold extracts can better promote cell proliferation and differentiation.					
31056297	14	113	theme	scaffold	2189:2196	arg1	extracts					2198:2205	the scaffold extracts	2185:2205	the scaffold extracts	2185:2205	CONCLUSIONS These findings suggest that compared with the Alg-Gel scaffold, 3D-printed Alg-Gel is more suitable for the growth of hDPSCs, and the scaffold extracts can better promote cell proliferation and differentiation.					
31056297	11	114	theme	alkaline	1836:1843	arg1	phosphatase					1845:1855	the alkaline phosphatase	1832:1855	the alkaline phosphatase staining	1832:1864	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	3	115	theme	Alg-Gel	479:485	arg1	scaffolds					487:495	both Alg-Gel and 3D-printed Alg-Gel scaffolds	451:495	both Alg-Gel and 3D-printed Alg-Gel scaffolds	451:495	METHODS hDPSCs were cultured in both Alg-Gel and 3D-printed Alg-Gel scaffolds.					
31056297	0	116	theme	Stem	138:141	arg1	Cells					143:147	Human Dental Pulp Stem Cells	120:147	Human Dental Pulp Stem Cells	120:147	Effects of 3-dimensional Bioprinting Alginate/Gelatin Hydrogel Scaffold Extract on Proliferation and Differentiation of Human Dental Pulp Stem Cells.					
31056297	14	117	dep	CONCLUSIONS	2043:2053	arg1	suggest					2070:2076	suggest	2070:2076	suggest that compared with the Alg-Gel scaffold, 3D-printed Alg-Gel is more suitable for the growth of hDPSCs, and the scaffold extracts can better promote cell proliferation and differentiation.	2070:2264	CONCLUSIONS These findings suggest that compared with the Alg-Gel scaffold, 3D-printed Alg-Gel is more suitable for the growth of hDPSCs, and the scaffold extracts can better promote cell proliferation and differentiation.					
31056297	11	118	theme	hDPSCs	1772:1777	arg1	differentiation					1753:1767	osteogenic/odontoblastic differentiation	1728:1767	osteogenic/odontoblastic differentiation of hDPSCs	1728:1777	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
31056297	11	119	theme	aqueous	1702:1708	arg1	extracts					1710:1717	Alg-Gel scaffold aqueous extracts	1685:1717	Alg-Gel scaffold aqueous extracts	1685:1717	Compared with the negative control group, 3D-printed Alg-Gel scaffold and Alg-Gel scaffold aqueous extracts promoted osteogenic/odontoblastic differentiation of hDPSCs with the enhanced formation of bone-like nodules and the alkaline phosphatase staining.					
30862178	2	0	from	harvesting	440:449	arg1	hip					474:476	the hip	470:476	the hip	470:476	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	10	1	theme	Col10	1893:1897	arg1	gene-expression					1874:1888	increasing gene-expression	1863:1888	increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123)	1863:1956	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	10	2	theme	FAI	1728:1730	arg1	samples					1732:1738	FAI samples	1728:1738	FAI samples	1728:1738	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	5	3	from	changes	951:957	arg1	cartilage					962:970	cartilage	962:970	cartilage of the hip joint	962:987	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	10	4	theme	increased	1768:1776	arg1	P					1784:1784	P = 0.0050	1784:1793	P = 0.0050	1784:1793	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	10	4	theme	increased	1768:1776	arg1	Col2					1778:1781	increased Col2	1768:1781	increased Col2 (P = 0.0050)	1768:1794	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	12	5	theme	diagnostic	2255:2264	arg1	arthroscopy					2266:2276	a diagnostic arthroscopy	2253:2276	a diagnostic arthroscopy	2253:2276	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	0	6	dep	Transplantation	23:37	arg1	Potential					110:118	Growth and Redifferentiation Potential	81:118	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.	0:184	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	10	7	theme	pellet	1753:1758	arg1	culture					1760:1766	pellet culture	1753:1766	pellet culture	1753:1766	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	1	8	theme	impingement	222:232	arg1	syndrome					240:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
30862178	10	9	theme	=	1786:1786	arg1	P					1784:1784	P = 0.0050	1784:1793	P = 0.0050	1784:1793	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	10	9	theme	=	1786:1786	arg1	Col2					1778:1781	increased Col2	1768:1781	increased Col2 (P = 0.0050)	1768:1794	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	1	10	theme	FAI	235:237	arg1	syndrome					240:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
30862178	11	11	theme	heterogeneous	2041:2053	arg1	composition					2055:2065	sufficient but heterogeneous composition	2026:2065	sufficient but heterogeneous composition relating to histological quality and chondrogenic potential	2026:2125	CONCLUSIONS Cartilage samples of patients with cam-type FAI showed sufficient but heterogeneous composition relating to histological quality and chondrogenic potential.					
30862178	4	12	with	patients	772:779	arg1	FAI					795:797	cam-type FAI	786:797	cam-type FAI	786:797	DESIGN Cartilage samples were gained from 15 patients with cam-type FAI undergoing arthroscopic femoral cam resection.					
30862178	1	13	from	damage	299:304	arg1	hip					313:315	the hip	309:315	the hip	309:315	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
30862178	2	14	theme	Autologous	318:327	arg1	transplantation					341:355	Autologous chondrocyte transplantation	318:355	Autologous chondrocyte transplantation	318:355	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	5	15	theme	hyaline	855:861	arg1	cartilage					863:871	hyaline cartilage	855:871	hyaline cartilage of the hip and knee joint	855:897	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	3	16	with	patients	708:715	arg1	FAI					722:724	FAI	722:724	FAI	722:724	Therefore, the goal of this study was to analyze the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI.					
30862178	11	17	theme	sufficient	2026:2035	arg1	composition					2055:2065	sufficient but heterogeneous composition	2026:2065	sufficient but heterogeneous composition relating to histological quality and chondrogenic potential	2026:2125	CONCLUSIONS Cartilage samples of patients with cam-type FAI showed sufficient but heterogeneous composition relating to histological quality and chondrogenic potential.					
30862178	10	18	theme	Col10	1800:1804	arg1	P					1818:1818	P = 0.0006	1818:1827	P = 0.0006	1818:1827	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	10	18	theme	Col10	1800:1804	arg1	expression					1806:1815	Col10 expression	1800:1815	Col10 expression (P = 0.0006)	1800:1828	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	9	19	theme	Col2	1587:1590	arg1	deposition					1573:1582	an increased deposition	1560:1582	an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261)	1560:1629	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	8	20	theme	Mankin	1370:1375	arg1	score					1377:1381	Mankin score	1370:1381	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10)	1362:1418	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	9	21	theme	P	1593:1593	arg1	0.0002					1597:1602	P = 0.0002	1593:1602	P = 0.0002	1593:1602	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	9	21	theme	P	1593:1593	arg1	Col2					1587:1590	Col2	1587:1590	Col2 (P = 0.0002)	1587:1603	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	4	22	theme	Cartilage	734:742	arg1	samples					744:750	DESIGN Cartilage samples	727:750	DESIGN Cartilage samples	727:750	DESIGN Cartilage samples were gained from 15 patients with cam-type FAI undergoing arthroscopic femoral cam resection.					
30862178	10	23	theme	=	1820:1820	arg1	P					1818:1818	P = 0.0006	1818:1827	P = 0.0006	1818:1827	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	10	23	theme	=	1820:1820	arg1	expression					1806:1815	Col10 expression	1800:1815	Col10 expression (P = 0.0006)	1800:1828	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	3	24	theme	redifferentiation	621:637	arg1	potential					639:647	the growth and redifferentiation potential	606:647	the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI	606:724	Therefore, the goal of this study was to analyze the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI.					
30862178	5	25	theme	arthritic	912:920	arg1	groups					930:935	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups	846:935	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8)	846:995	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	0	26	theme	Growth	81:86	arg1	Potential					110:118	Growth and Redifferentiation Potential	81:118	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.	0:184	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	3	27	theme	growth	610:615	arg1	potential					639:647	the growth and redifferentiation potential	606:647	the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI	606:724	Therefore, the goal of this study was to analyze the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI.					
30862178	0	28	from	Transplantation	23:37	arg1	Syndrome					71:78	Femoroacetabular Impingement Syndrome	42:78	Femoroacetabular Impingement Syndrome	42:78	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	10	29	theme	=	1937:1937	arg1	r					1935:1935	r = 0.8829	1935:1944	r = 0.8829	1935:1944	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	10	29	theme	=	1937:1937	arg1	RunX2					1928:1932	RunX2	1928:1932	RunX2 (r = 0.8829, P = 0.123)	1928:1956	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	0	30	theme	Redifferentiation	92:108	arg1	Potential					110:118	Growth and Redifferentiation Potential	81:118	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.	0:184	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	1	31	theme	Cam-type	196:203	arg1	syndrome					240:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
30862178	2	32	theme	cam	544:546	arg1	lesions					548:554	femoral cam lesions	536:554	femoral cam lesions	536:554	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	9	33	theme	=	1621:1621	arg1	P					1619:1619	P = 0.0261	1619:1628	P = 0.0261	1619:1628	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	9	33	theme	=	1621:1621	arg1	aggrecan					1609:1616	aggrecan	1609:1616	aggrecan (P = 0.0261)	1609:1629	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	8	34	theme	wide	1429:1432	arg1	variation					1434:1442	a wide variation	1427:1442	a wide variation	1427:1442	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	10	35	theme	=	1949:1949	arg1	P					1947:1947	P = 0.123	1947:1955	P = 0.123	1947:1955	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	4	36	theme	cam-type	786:793	arg1	FAI					795:797	cam-type FAI	786:797	cam-type FAI	786:797	DESIGN Cartilage samples were gained from 15 patients with cam-type FAI undergoing arthroscopic femoral cam resection.					
30862178	0	37	theme	Impingement	59:69	arg1	Syndrome					71:78	Femoroacetabular Impingement Syndrome	42:78	Femoroacetabular Impingement Syndrome	42:78	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	12	38	theme	degeneration	2324:2335	arg1	stage					2305:2309	individual preexisting stage	2282:2309	individual preexisting stage	2282:2309	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	12	38	theme	degeneration	2324:2335	arg1	arthroscopy					2266:2276	a diagnostic arthroscopy	2253:2276	a diagnostic arthroscopy	2253:2276	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	2	39	theme	chondral	413:420	arg1	defects					422:428	focal chondral defects	407:428	focal chondral defects	407:428	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	0	40	theme	Autologous	0:9	arg1	Transplantation					23:37	Autologous Chondrocyte Transplantation	0:37	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.	0:184	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	7	41	theme	transcription-polymerase	1278:1301	arg1	qRT-PCR					1319:1325	qRT-PCR	1319:1325	qRT-PCR	1319:1325	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	41	theme	transcription-polymerase	1278:1301	arg1	reaction					1309:1316	quantitative reverse transcription-polymerase chain reaction	1257:1316	quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2)	1257:1359	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	9	42	theme	pellet	1637:1642	arg1	culture					1644:1650	pellet culture	1637:1650	pellet culture	1637:1650	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	12	43	theme	appropriate	2341:2351	arg1	conditions					2370:2379	appropriate pellet-culturing conditions	2341:2379	appropriate pellet-culturing conditions	2341:2379	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	5	44	theme	Healthy	846:852	arg1	groups					930:935	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups	846:935	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8)	846:995	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	5	45	theme	degenerative	938:949	arg1	changes					951:957	degenerative changes	938:957	degenerative changes in cartilage of the hip joint	938:987	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	10	46	dep	r	1935:1935	arg1	P					1947:1947	P = 0.123	1947:1955	P = 0.123	1947:1955	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	7	47	theme	quantitative	1257:1268	arg1	qRT-PCR					1319:1325	qRT-PCR	1319:1325	qRT-PCR	1319:1325	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	47	theme	quantitative	1257:1268	arg1	reaction					1309:1316	quantitative reverse transcription-polymerase chain reaction	1257:1316	quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2)	1257:1359	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	48	dep	immunohistochemistry	1202:1221	arg1	Col2					1236:1239	Col2	1236:1239	Col2	1236:1239	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	48	dep	immunohistochemistry	1202:1221	arg1	Col1					1230:1233	Col1	1230:1233	Col1	1230:1233	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	48	dep	immunohistochemistry	1202:1221	arg1	aggrecan					1242:1249	aggrecan	1242:1249	aggrecan	1242:1249	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	3	49	theme	samples	662:668	arg1	potential					639:647	the growth and redifferentiation potential	606:647	the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI	606:724	Therefore, the goal of this study was to analyze the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI.					
30862178	0	50	theme	Femoroacetabular	42:57	arg1	Syndrome					71:78	Femoroacetabular Impingement Syndrome	42:78	Femoroacetabular Impingement Syndrome	42:78	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	8	51	dep	FAI-samples	1386:1396	arg1	4.1±3.1					1399:1405	4.1±3.1	1399:1405	4.1±3.1	1399:1405	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	8	51	dep	FAI-samples	1386:1396	arg1	Range					1408:1412	Range 0-10	1408:1417	Range 0-10	1408:1417	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	12	52	theme	cam	2173:2175	arg1	lesion					2177:2182	the cam lesion	2169:2182	the cam lesion	2169:2182	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	11	53	with	patients	1992:1999	arg1	FAI					2015:2017	cam-type FAI	2006:2017	cam-type FAI	2006:2017	CONCLUSIONS Cartilage samples of patients with cam-type FAI showed sufficient but heterogeneous composition relating to histological quality and chondrogenic potential.					
30862178	7	54	dep	reaction	1309:1316	arg1	Sox9					1348:1351	Sox9	1348:1351	Sox9	1348:1351	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	54	dep	reaction	1309:1316	arg1	Col10					1341:1345	Col10	1341:1345	Col10	1341:1345	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	54	dep	reaction	1309:1316	arg1	Col2					1335:1338	Col2	1335:1338	Col2	1335:1338	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	54	dep	reaction	1309:1316	arg1	RunX2					1354:1358	RunX2	1354:1358	RunX2	1354:1358	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	54	dep	reaction	1309:1316	arg1	Col1					1329:1332	Col1	1329:1332	Col1	1329:1332	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	6	55	theme	pellet	1094:1099	arg1	conditions					1101:1110	pellet conditions	1094:1110	pellet conditions	1094:1110	Chondrocytes were initially cultured under monolayer, and subsequently under pellet conditions.					
30862178	9	56	theme	monolayer	1683:1691	arg1	culture					1693:1699	monolayer culture	1683:1699	monolayer culture	1683:1699	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	3	57	theme	cam	689:691	arg1	deformities					693:703	the cam deformities	685:703	the cam deformities in patients with FAI	685:724	Therefore, the goal of this study was to analyze the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI.					
30862178	2	58	theme	high	368:371	arg1	rates					381:385	high success rates	368:385	high success rates	368:385	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	5	59	theme	hip	979:981	arg1	joint					983:987	the hip joint	975:987	the hip joint	975:987	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	12	60	theme	cartilage	2314:2322	arg1	degeneration					2324:2335	cartilage degeneration	2314:2335	cartilage degeneration	2314:2335	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	11	61	theme	patients	1992:1999	arg1	samples					1981:1987	CONCLUSIONS Cartilage samples	1959:1987	CONCLUSIONS Cartilage samples of patients with cam-type FAI	1959:2017	CONCLUSIONS Cartilage samples of patients with cam-type FAI showed sufficient but heterogeneous composition relating to histological quality and chondrogenic potential.					
30862178	4	62	theme	femoral	823:829	arg1	resection					835:843	arthroscopic femoral cam resection	810:843	arthroscopic femoral cam resection	810:843	DESIGN Cartilage samples were gained from 15 patients with cam-type FAI undergoing arthroscopic femoral cam resection.					
30862178	5	63	theme	hip	880:882	arg1	cartilage					863:871	hyaline cartilage	855:871	hyaline cartilage of the hip and knee joint	855:897	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	9	64	from	deposition	1666:1675	arg1	groups					1708:1713	all groups	1704:1713	all groups	1704:1713	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	11	65	theme	chondrogenic	2104:2115	arg1	potential					2117:2125	chondrogenic potential	2104:2125	chondrogenic potential	2104:2125	CONCLUSIONS Cartilage samples of patients with cam-type FAI showed sufficient but heterogeneous composition relating to histological quality and chondrogenic potential.					
30862178	0	66	from	Femur	155:159	arg1	Deformities					173:183	Cam-Type Deformities	164:183	Cam-Type Deformities	164:183	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	1	67	theme	reasons	277:283	arg1	reasons					277:283	the most frequent reasons	259:283	the most frequent reasons for cartilage damage in the hip	259:315	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
30862178	1	67	theme	reasons	277:283	arg1	one					252:254	one	252:254	one	252:254	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
30862178	5	68	dep	Healthy	846:852	arg1	=					902:902	=	902:902	=	902:902	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	12	69	theme	individual	2282:2291	arg1	stage					2305:2309	individual preexisting stage	2282:2309	individual preexisting stage	2282:2309	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	1	70	theme	cartilage	289:297	arg1	damage					299:304	cartilage damage	289:304	cartilage damage in the hip	289:315	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
30862178	11	71	theme	cam-type	2006:2013	arg1	FAI					2015:2017	cam-type FAI	2006:2017	cam-type FAI	2006:2017	CONCLUSIONS Cartilage samples of patients with cam-type FAI showed sufficient but heterogeneous composition relating to histological quality and chondrogenic potential.					
30862178	12	72	from	harvesting	2137:2146	arg1	lesion					2177:2182	the cam lesion	2169:2182	the cam lesion	2169:2182	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	1	73	theme	femoroacetabular	205:220	arg1	syndrome					240:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
30862178	10	74	theme	=	1902:1902	arg1	r					1900:1900	r = 0.8108	1900:1909	r = 0.8108	1900:1909	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	10	74	theme	=	1902:1902	arg1	Col10					1893:1897	Col10	1893:1897	Col10 (r = 0.8108, P = 0.0341)	1893:1922	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	5	75	dep	=	992:992	arg1	changes					951:957	degenerative changes	938:957	degenerative changes in cartilage of the hip joint	938:987	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	2	76	theme	chondrocyte	329:339	arg1	transplantation					341:355	Autologous chondrocyte transplantation	318:355	Autologous chondrocyte transplantation	318:355	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	9	77	theme	increased	1563:1571	arg1	deposition					1573:1582	an increased deposition	1560:1582	an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261)	1560:1629	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	10	78	theme	=	1914:1914	arg1	P					1912:1912	P = 0.0341	1912:1921	P = 0.0341	1912:1921	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	3	79	from	deformities	693:703	arg1	patients					708:715	patients	708:715	patients with FAI	708:724	Therefore, the goal of this study was to analyze the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI.					
30862178	0	80	theme	Cam-Type	164:171	arg1	Deformities					173:183	Cam-Type Deformities	164:183	Cam-Type Deformities	164:183	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	5	81	theme	knee	888:891	arg1	cartilage					863:871	hyaline cartilage	855:871	hyaline cartilage of the hip and knee joint	855:897	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	10	82	theme	increasing	1863:1872	arg1	gene-expression					1874:1888	increasing gene-expression	1863:1888	increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123)	1863:1956	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	2	83	theme	lesions	548:554	arg1	region					526:531	the region	522:531	the region of femoral cam lesions	522:554	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	8	84	theme	FAI-samples	1386:1396	arg1	score					1377:1381	Mankin score	1370:1381	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10)	1362:1418	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	7	85	theme	Mankin	1173:1178	arg1	score					1180:1184	Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2)	1173:1359	score	1180:1184	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	3	86	theme	study	585:589	arg1	goal					572:575	the goal	568:575	the goal of this study	568:589	Therefore, the goal of this study was to analyze the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI.					
30862178	8	87	dep	RESULTS	1362:1368	arg1	score					1377:1381	Mankin score	1370:1381	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10)	1362:1418	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	10	88	theme	RunX2	1928:1932	arg1	gene-expression					1874:1888	increasing gene-expression	1863:1888	increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123)	1863:1956	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	12	89	from	lesion	2177:2182	arg1	chondrocytes					2151:2162	chondrocytes	2151:2162	chondrocytes from the cam lesion	2151:2182	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	12	89	from	lesion	2177:2182	arg1	harvesting					2137:2146	harvesting	2137:2146	harvesting of chondrocytes from the cam lesion	2137:2182	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	12	89	from	lesion	2177:2182	arg1	option					2201:2206	a valid option	2193:2206	a valid option	2193:2206	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	9	90	theme	=	1595:1595	arg1	0.0002					1597:1602	P = 0.0002	1593:1602	P = 0.0002	1593:1602	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	9	90	theme	=	1595:1595	arg1	Col2					1587:1590	Col2	1587:1590	Col2 (P = 0.0002)	1587:1603	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	4	91	theme	DESIGN	727:732	arg1	samples					744:750	DESIGN Cartilage samples	727:750	DESIGN Cartilage samples	727:750	DESIGN Cartilage samples were gained from 15 patients with cam-type FAI undergoing arthroscopic femoral cam resection.					
30862178	8	92	dep	significant	1452:1462	arg1	lower					1464:1468	lower	1464:1468	lower	1464:1468	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	8	92	dep	significant	1452:1462	arg1	=					1473:1473	=	1473:1473	=	1473:1473	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	12	93	theme	preexisting	2293:2303	arg1	stage					2305:2309	individual preexisting stage	2282:2309	individual preexisting stage	2282:2309	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	1	94	theme	OBJECTIVE	186:194	arg1	syndrome					240:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome	186:247	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
30862178	9	95	theme	aggrecan	1609:1616	arg1	deposition					1573:1582	an increased deposition	1560:1582	an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261)	1560:1629	IHC showed an increased deposition of Col2 (P = 0.0002) and aggrecan (P = 0.0261) after pellet culture compared with deposition after monolayer culture in all groups.					
30862178	5	96	dep	groups	930:935	arg1	=					992:992	=	992:992	=	992:992	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	0	97	theme	Chondrocytes	123:134	arg1	Potential					110:118	Growth and Redifferentiation Potential	81:118	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.	0:184	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	8	98	dep	control	1516:1522	arg1	range					1536:1540	range 4-12	1536:1545	range 4-12	1536:1545	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	8	98	dep	control	1516:1522	arg1	±					1529:1529	7.5 ± 2.7	1525:1533	7.5 ± 2.7	1525:1533	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	2	99	theme	femoral	536:542	arg1	lesions					548:554	femoral cam lesions	536:554	femoral cam lesions	536:554	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	7	100	theme	chain	1303:1307	arg1	qRT-PCR					1319:1325	qRT-PCR	1319:1325	qRT-PCR	1319:1325	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	100	theme	chain	1303:1307	arg1	reaction					1309:1316	quantitative reverse transcription-polymerase chain reaction	1257:1316	quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2)	1257:1359	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	11	101	theme	Cartilage	1971:1979	arg1	samples					1981:1987	CONCLUSIONS Cartilage samples	1959:1987	CONCLUSIONS Cartilage samples of patients with cam-type FAI	1959:2017	CONCLUSIONS Cartilage samples of patients with cam-type FAI showed sufficient but heterogeneous composition relating to histological quality and chondrogenic potential.					
30862178	2	102	theme	defects	422:428	arg1	treatment					394:402	the treatment	390:402	the treatment of focal chondral defects	390:428	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	0	103	theme	Chondrocyte	11:21	arg1	Transplantation					23:37	Autologous Chondrocyte Transplantation	0:37	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.	0:184	Autologous Chondrocyte Transplantation in Femoroacetabular Impingement Syndrome: Growth and Redifferentiation Potential of Chondrocytes Harvested from the Femur in Cam-Type Deformities.					
30862178	5	104	theme	control	922:928	arg1	groups					930:935	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups	846:935	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8)	846:995	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	7	105	theme	reverse	1270:1276	arg1	qRT-PCR					1319:1325	qRT-PCR	1319:1325	qRT-PCR	1319:1325	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	7	105	theme	reverse	1270:1276	arg1	reaction					1309:1316	quantitative reverse transcription-polymerase chain reaction	1257:1316	quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2)	1257:1359	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	12	106	theme	valid	2195:2199	arg1	harvesting					2137:2146	harvesting	2137:2146	harvesting of chondrocytes from the cam lesion	2137:2182	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	12	106	theme	valid	2195:2199	arg1	option					2201:2206	a valid option	2193:2206	a valid option	2193:2206	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	12	107	theme	pellet-culturing	2353:2368	arg1	conditions					2370:2379	appropriate pellet-culturing conditions	2341:2379	appropriate pellet-culturing conditions	2341:2379	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	5	108	dep	=	902:902	arg1	cartilage					863:871	hyaline cartilage	855:871	hyaline cartilage of the hip and knee joint	855:897	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	2	109	theme	focal	407:411	arg1	defects					422:428	focal chondral defects	407:428	focal chondral defects	407:428	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	2	110	theme	chondrocytes	454:465	arg1	harvesting					440:449	harvesting	440:449	harvesting of chondrocytes in the hip	440:476	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	3	111	theme	cartilage	652:660	arg1	samples					662:668	cartilage samples	652:668	cartilage samples harvested from the cam deformities in patients with FAI	652:724	Therefore, the goal of this study was to analyze the growth and redifferentiation potential of cartilage samples harvested from the cam deformities in patients with FAI.					
30862178	12	112	theme	cartilage	2224:2232	arg1	lesion					2234:2239	a cartilage lesion	2222:2239	a cartilage lesion	2222:2239	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	7	113	theme	comparative	1115:1125	arg1	representation					1127:1140	A comparative representation	1113:1140	A comparative representation of the groups	1113:1154	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	4	114	theme	cam	831:833	arg1	resection					835:843	arthroscopic femoral cam resection	810:843	arthroscopic femoral cam resection	810:843	DESIGN Cartilage samples were gained from 15 patients with cam-type FAI undergoing arthroscopic femoral cam resection.					
30862178	5	115	dep	hip	880:882	arg1	joint					893:897	joint	893:897	joint	893:897	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	5	115	dep	hip	880:882	arg1	the					876:878	the	876:878	the	876:878	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	2	116	theme	success	373:379	arg1	rates					381:385	high success rates	368:385	high success rates	368:385	Autologous chondrocyte transplantation has proven high success rates in the treatment of focal chondral defects; however, harvesting of chondrocytes in the hip has been reported but not specifically from the region of femoral cam lesions.					
30862178	11	117	theme	CONCLUSIONS	1959:1969	arg1	samples					1981:1987	CONCLUSIONS Cartilage samples	1959:1987	CONCLUSIONS Cartilage samples of patients with cam-type FAI	1959:2017	CONCLUSIONS Cartilage samples of patients with cam-type FAI showed sufficient but heterogeneous composition relating to histological quality and chondrogenic potential.					
30862178	11	118	theme	histological	2079:2090	arg1	quality					2092:2098	histological quality	2079:2098	histological quality	2079:2098	CONCLUSIONS Cartilage samples of patients with cam-type FAI showed sufficient but heterogeneous composition relating to histological quality and chondrogenic potential.					
30862178	12	119	theme	chondrocytes	2151:2162	arg1	harvesting					2137:2146	harvesting	2137:2146	harvesting of chondrocytes from the cam lesion	2137:2182	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	12	119	theme	chondrocytes	2151:2162	arg1	option					2201:2206	a valid option	2193:2206	a valid option	2193:2206	However, harvesting of chondrocytes from the cam lesion might be a valid option especially if a cartilage lesion is noted in a diagnostic arthroscopy and individual preexisting stage of cartilage degeneration and appropriate pellet-culturing conditions are considered.					
30862178	7	120	dep	score	1180:1184	arg1	classification					1186:1199	classification	1186:1199	classification	1186:1199	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	5	121	theme	joint	983:987	arg1	cartilage					962:970	cartilage	962:970	cartilage of the hip joint	962:987	Healthy (hyaline cartilage of the hip and knee joint, n = 12) and arthritic control groups (degenerative changes in cartilage of the hip joint, n = 8) were also analyzed.					
30862178	10	122	dep	r	1900:1900	arg1	P					1912:1912	P = 0.0341	1912:1921	P = 0.0341	1912:1921	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	10	123	theme	Mankin	1839:1844	arg1	score					1846:1850	Mankin score	1839:1850	Mankin score	1839:1850	In qRT-PCR, FAI samples showed after pellet culture increased Col2 (P = 0.0050) and Col10 expression (P = 0.0006) and also Mankin score correlated increasing gene-expression of Col10 (r = 0.8108, P = 0.0341) and RunX2 (r = 0.8829, P = 0.123).					
30862178	8	124	theme	arthritic	1506:1514	arg1	control					1516:1522	the arthritic control	1502:1522	the arthritic control (7.5 ± 2.7, range 4-12)	1502:1546	RESULTS Mankin score of FAI-samples (4.1±3.1, Range 0-10) showed a wide variation but was significant lower (P = 0.0244) when compared with the arthritic control (7.5 ± 2.7, range 4-12).					
30862178	4	125	theme	arthroscopic	810:821	arg1	resection					835:843	arthroscopic femoral cam resection	810:843	arthroscopic femoral cam resection	810:843	DESIGN Cartilage samples were gained from 15 patients with cam-type FAI undergoing arthroscopic femoral cam resection.					
30862178	7	126	theme	groups	1149:1154	arg1	representation					1127:1140	A comparative representation	1113:1140	A comparative representation of the groups	1113:1154	A comparative representation of the groups was performed by Mankin score classification, immunohistochemistry (IHC) (Col1, Col2, aggrecan), and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) (Col1, Col2, Col10, Sox9, RunX2).					
30862178	1	127	theme	frequent	268:275	arg1	reasons					277:283	the most frequent reasons	259:283	the most frequent reasons for cartilage damage in the hip	259:315	OBJECTIVE Cam-type femoroacetabular impingement (FAI) syndrome is one of the most frequent reasons for cartilage damage in the hip.					
31739024	0	0	theme	Pb	88:89	arg1	removal					77:83	efficient removal	67:83	efficient removal of Pb(II), Ni(II) and Cu(II) from water	67:123	Alginate modified graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water.					
31739024	4	1	theme	different	628:636	arg1	conditions					638:647	different conditions	628:647	different conditions	628:647	The effects of different conditions were examined such as pH, initial concentration and contact time.					
31739024	8	2	theme	capacity	1453:1460	arg1	loss					1462:1465	obvious adsorption capacity loss	1434:1465	obvious adsorption capacity loss	1434:1465	More significantly, g-C3N4/SA showed excellent regeneration ability and repeatedly utilized at least five cycles without obvious adsorption capacity loss.					
31739024	2	3	theme	composite	282:290	arg1	g-C3N4/SA					303:311	g-C3N4/SA	303:311	g-C3N4/SA	303:311	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	2	3	theme	composite	282:290	arg1	hydrogels					292:300	alginate modified graphitic carbon nitride composite hydrogels	239:300	alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA)	239:312	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	1	4	theme	great	164:168	arg1	significance					170:181	great significance	164:181	great significance	164:181	The removal of heavy metal ions is of great significance to the friendly development of the environment.					
31739024	6	5	theme	adsorption	994:1003	arg1	capacity					1005:1012	The calculated maximum adsorption capacity	971:1012	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II)	971:1041	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	6	5	theme	adsorption	994:1003	arg1	306.3					1055:1059	306.3	1055:1059	306.3	1055:1059	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	2	6	theme	nitride	274:280	arg1	g-C3N4/SA					303:311	g-C3N4/SA	303:311	g-C3N4/SA	303:311	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	2	6	theme	nitride	274:280	arg1	hydrogels					292:300	alginate modified graphitic carbon nitride composite hydrogels	239:300	alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA)	239:312	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	8	7	theme	excellent	1350:1358	arg1	ability					1373:1379	excellent regeneration ability	1350:1379	excellent regeneration ability	1350:1379	More significantly, g-C3N4/SA showed excellent regeneration ability and repeatedly utilized at least five cycles without obvious adsorption capacity loss.					
31739024	5	8	dep	recycling	864:872	arg1	the					860:862	the	860:862	the	860:862	It demonstrated that this integration process significantly improved the adsorption capacity of each component to targeted metal ions and solved the recycling and separation problem of g-C3N4, which is powdery sorbent and hard to recycle after adsorption.					
31739024	6	9	theme	Cu	1036:1037	arg1	capacity					1005:1012	The calculated maximum adsorption capacity	971:1012	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II)	971:1041	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	6	9	theme	Cu	1036:1037	arg1	306.3					1055:1059	306.3	1055:1059	306.3	1055:1059	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	5	10	theme	separation	878:887	arg1	problem					889:895	separation problem	878:895	separation problem	878:895	It demonstrated that this integration process significantly improved the adsorption capacity of each component to targeted metal ions and solved the recycling and separation problem of g-C3N4, which is powdery sorbent and hard to recycle after adsorption.					
31739024	8	11	theme	adsorption	1442:1451	arg1	loss					1462:1465	obvious adsorption capacity loss	1434:1465	obvious adsorption capacity loss	1434:1465	More significantly, g-C3N4/SA showed excellent regeneration ability and repeatedly utilized at least five cycles without obvious adsorption capacity loss.					
31739024	7	12	dep	model	1194:1198	arg1	data					1227:1230	kinetic data	1219:1230	kinetic data	1219:1230	Adsorption isotherms studies showed that the equilibrium adsorption data fitted well to the Langmuir model and the adsorption kinetic data described well with the pseudo-second-order model according to kinetics studies.					
31739024	3	13	theme	scanning	499:506	arg1	microscopy					517:526	scanning electron microscopy	499:526	scanning electron microscopy (SEM)	499:532	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	13	theme	scanning	499:506	arg1	spectroscopy					478:489	spectroscopy	478:489	spectroscopy (FTIR)	478:496	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	13	theme	scanning	499:506	arg1	SEM					529:531	SEM	529:531	SEM	529:531	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	9	14	theme	contaminated	1604:1615	arg1	water					1617:1621	contaminated water	1604:1621	contaminated water	1604:1621	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	8	15	theme	obvious	1434:1440	arg1	loss					1462:1465	obvious adsorption capacity loss	1434:1465	obvious adsorption capacity loss	1434:1465	More significantly, g-C3N4/SA showed excellent regeneration ability and repeatedly utilized at least five cycles without obvious adsorption capacity loss.					
31739024	9	16	theme	Ni	1576:1577	arg1	adsorption					1554:1563	highly effective adsorption	1537:1563	highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water	1537:1621	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	3	17	dep	Fourier	451:457	arg1	transform					459:467	transform	459:467	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS)	459:610	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	6	18	theme	Pb	1017:1018	arg1	capacity					1005:1012	The calculated maximum adsorption capacity	971:1012	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II)	971:1041	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	6	18	theme	Pb	1017:1018	arg1	306.3					1055:1059	306.3	1055:1059	306.3	1055:1059	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	7	19	theme	Adsorption	1093:1102	arg1	studies					1114:1120	Adsorption isotherms studies	1093:1120	Adsorption isotherms studies	1093:1120	Adsorption isotherms studies showed that the equilibrium adsorption data fitted well to the Langmuir model and the adsorption kinetic data described well with the pseudo-second-order model according to kinetics studies.					
31739024	3	20	dep	transform	459:467	arg1	infrared					469:476	infrared	469:476	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS)	459:610	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	5	21	theme	component	816:824	arg1	capacity					799:806	the adsorption capacity	784:806	the adsorption capacity of each component to targeted metal ions	784:847	It demonstrated that this integration process significantly improved the adsorption capacity of each component to targeted metal ions and solved the recycling and separation problem of g-C3N4, which is powdery sorbent and hard to recycle after adsorption.					
31739024	5	22	theme	integration	741:751	arg1	process					753:759	this integration process	736:759	this integration process	736:759	It demonstrated that this integration process significantly improved the adsorption capacity of each component to targeted metal ions and solved the recycling and separation problem of g-C3N4, which is powdery sorbent and hard to recycle after adsorption.					
31739024	1	23	theme	friendly	190:197	arg1	development					199:209	the friendly development	186:209	the friendly development of the environment	186:228	The removal of heavy metal ions is of great significance to the friendly development of the environment.					
31739024	0	24	theme	carbon	28:33	arg1	hydrogels					53:61	graphitic carbon nitride composite hydrogels	18:61	graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water	18:123	Alginate modified graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water.					
31739024	0	25	from	water	119:123	arg1	removal					77:83	efficient removal	67:83	efficient removal of Pb(II), Ni(II) and Cu(II) from water	67:123	Alginate modified graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water.					
31739024	8	26	theme	regeneration	1360:1371	arg1	ability					1373:1379	excellent regeneration ability	1350:1379	excellent regeneration ability	1350:1379	More significantly, g-C3N4/SA showed excellent regeneration ability and repeatedly utilized at least five cycles without obvious adsorption capacity loss.					
31739024	7	27	theme	pseudo-second-order	1256:1274	arg1	model					1276:1280	the pseudo-second-order model	1252:1280	the pseudo-second-order model according to kinetics studies	1252:1310	Adsorption isotherms studies showed that the equilibrium adsorption data fitted well to the Langmuir model and the adsorption kinetic data described well with the pseudo-second-order model according to kinetics studies.					
31739024	3	28	theme	electron	508:515	arg1	microscopy					517:526	scanning electron microscopy	499:526	scanning electron microscopy (SEM)	499:532	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	28	theme	electron	508:515	arg1	spectroscopy					478:489	spectroscopy	478:489	spectroscopy (FTIR)	478:496	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	28	theme	electron	508:515	arg1	SEM					529:531	SEM	529:531	SEM	529:531	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	9	29	theme	potential	1515:1523	arg1	g-C3N4/SA					1482:1490	As-fabricated g-C3N4/SA	1468:1490	As-fabricated g-C3N4/SA	1468:1490	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	9	29	theme	potential	1515:1523	arg1	sorbent					1525:1531	a potential sorbent	1513:1531	a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water	1513:1621	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	2	30	theme	facile	354:359	arg1	method					390:395	a facile cross-linking polymerization method	352:395	a facile cross-linking polymerization method	352:395	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	2	31	theme	cross-linking	361:373	arg1	method					390:395	a facile cross-linking polymerization method	352:395	a facile cross-linking polymerization method	352:395	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	0	32	theme	Ni	96:97	arg1	removal					77:83	efficient removal	67:83	efficient removal of Pb(II), Ni(II) and Cu(II) from water	67:123	Alginate modified graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water.					
31739024	3	33	theme	photoelectron	579:591	arg1	XPS					607:609	XPS	607:609	XPS	607:609	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	33	theme	photoelectron	579:591	arg1	spectroscopy					593:604	X-ray photoelectron spectroscopy	573:604	X-ray photoelectron spectroscopy (XPS)	573:610	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	33	theme	photoelectron	579:591	arg1	spectroscopy					478:489	spectroscopy	478:489	spectroscopy (FTIR)	478:496	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	5	34	theme	g-C3N4	900:905	arg1	recycling					864:872	recycling	864:872	recycling	864:872	It demonstrated that this integration process significantly improved the adsorption capacity of each component to targeted metal ions and solved the recycling and separation problem of g-C3N4, which is powdery sorbent and hard to recycle after adsorption.					
31739024	5	34	theme	g-C3N4	900:905	arg1	problem					889:895	separation problem	878:895	separation problem	878:895	It demonstrated that this integration process significantly improved the adsorption capacity of each component to targeted metal ions and solved the recycling and separation problem of g-C3N4, which is powdery sorbent and hard to recycle after adsorption.					
31739024	6	35	theme	maximum	986:992	arg1	capacity					1005:1012	The calculated maximum adsorption capacity	971:1012	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II)	971:1041	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	6	35	theme	maximum	986:992	arg1	306.3					1055:1059	306.3	1055:1059	306.3	1055:1059	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	0	36	theme	composite	43:51	arg1	hydrogels					53:61	graphitic carbon nitride composite hydrogels	18:61	graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water	18:123	Alginate modified graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water.					
31739024	4	37	theme	conditions	638:647	arg1	effects					617:623	The effects	613:623	The effects of different conditions	613:647	The effects of different conditions were examined such as pH, initial concentration and contact time.					
31739024	6	38	theme	calculated	975:984	arg1	capacity					1005:1012	The calculated maximum adsorption capacity	971:1012	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II)	971:1041	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	6	38	theme	calculated	975:984	arg1	306.3					1055:1059	306.3	1055:1059	306.3	1055:1059	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	3	39	theme	energy	535:540	arg1	spectroscopy					478:489	spectroscopy	478:489	spectroscopy (FTIR)	478:496	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	39	theme	energy	535:540	arg1	EDS					567:569	EDS	567:569	EDS	567:569	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	39	theme	energy	535:540	arg1	spectroscopy					553:564	energy dispersive spectroscopy	535:564	energy dispersive spectroscopy (EDS)	535:570	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	5	40	theme	adsorption	788:797	arg1	capacity					799:806	the adsorption capacity	784:806	the adsorption capacity of each component to targeted metal ions	784:847	It demonstrated that this integration process significantly improved the adsorption capacity of each component to targeted metal ions and solved the recycling and separation problem of g-C3N4, which is powdery sorbent and hard to recycle after adsorption.					
31739024	0	41	theme	nitride	35:41	arg1	hydrogels					53:61	graphitic carbon nitride composite hydrogels	18:61	graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water	18:123	Alginate modified graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water.					
31739024	1	42	theme	environment	218:228	arg1	development					199:209	the friendly development	186:209	the friendly development of the environment	186:228	The removal of heavy metal ions is of great significance to the friendly development of the environment.					
31739024	2	43	theme	polymerization	375:388	arg1	method					390:395	a facile cross-linking polymerization method	352:395	a facile cross-linking polymerization method	352:395	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	1	44	theme	heavy	141:145	arg1	ions					153:156	heavy metal ions	141:156	heavy metal ions	141:156	The removal of heavy metal ions is of great significance to the friendly development of the environment.					
31739024	8	45	used	utilized	1396:1403	arg2	g-C3N4/SA					1333:1341	g-C3N4/SA	1333:1341	g-C3N4/SA	1333:1341	More significantly, g-C3N4/SA showed excellent regeneration ability and repeatedly utilized at least five cycles without obvious adsorption capacity loss.					
31739024	0	46	theme	Cu	107:108	arg1	removal					77:83	efficient removal	67:83	efficient removal of Pb(II), Ni(II) and Cu(II) from water	67:123	Alginate modified graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water.					
31739024	3	47	theme	X-ray	573:577	arg1	XPS					607:609	XPS	607:609	XPS	607:609	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	47	theme	X-ray	573:577	arg1	spectroscopy					593:604	X-ray photoelectron spectroscopy	573:604	X-ray photoelectron spectroscopy (XPS)	573:610	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	47	theme	X-ray	573:577	arg1	spectroscopy					478:489	spectroscopy	478:489	spectroscopy (FTIR)	478:496	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	6	48	theme	Ni	1025:1026	arg1	capacity					1005:1012	The calculated maximum adsorption capacity	971:1012	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II)	971:1041	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	6	48	theme	Ni	1025:1026	arg1	306.3					1055:1059	306.3	1055:1059	306.3	1055:1059	The calculated maximum adsorption capacity of Pb(II), Ni(II) and Cu(II) were 383.4, 306.3 and 168.2 mg g-1, respectively.					
31739024	2	49	theme	carbon	267:272	arg1	g-C3N4/SA					303:311	g-C3N4/SA	303:311	g-C3N4/SA	303:311	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	2	49	theme	carbon	267:272	arg1	hydrogels					292:300	alginate modified graphitic carbon nitride composite hydrogels	239:300	alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA)	239:312	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	9	50	theme	ions	1594:1597	arg1	adsorption					1554:1563	highly effective adsorption	1537:1563	highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water	1537:1621	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	7	51	theme	kinetics	1295:1302	arg1	studies					1304:1310	kinetics studies	1295:1310	kinetics studies	1295:1310	Adsorption isotherms studies showed that the equilibrium adsorption data fitted well to the Langmuir model and the adsorption kinetic data described well with the pseudo-second-order model according to kinetics studies.					
31739024	7	52	theme	kinetic	1219:1225	arg1	data					1227:1230	kinetic data	1219:1230	kinetic data	1219:1230	Adsorption isotherms studies showed that the equilibrium adsorption data fitted well to the Langmuir model and the adsorption kinetic data described well with the pseudo-second-order model according to kinetics studies.					
31739024	2	53	theme	graphitic	257:265	arg1	g-C3N4/SA					303:311	g-C3N4/SA	303:311	g-C3N4/SA	303:311	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	2	53	theme	graphitic	257:265	arg1	hydrogels					292:300	alginate modified graphitic carbon nitride composite hydrogels	239:300	alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA)	239:312	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	7	54	theme	equilibrium	1138:1148	arg1	data					1161:1164	the equilibrium adsorption data	1134:1164	the equilibrium adsorption data	1134:1164	Adsorption isotherms studies showed that the equilibrium adsorption data fitted well to the Langmuir model and the adsorption kinetic data described well with the pseudo-second-order model according to kinetics studies.					
31739024	9	55	theme	effective	1544:1552	arg1	adsorption					1554:1563	highly effective adsorption	1537:1563	highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water	1537:1621	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	3	56	theme	dispersive	542:551	arg1	spectroscopy					478:489	spectroscopy	478:489	spectroscopy (FTIR)	478:496	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	56	theme	dispersive	542:551	arg1	EDS					567:569	EDS	567:569	EDS	567:569	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	56	theme	dispersive	542:551	arg1	spectroscopy					553:564	energy dispersive spectroscopy	535:564	energy dispersive spectroscopy (EDS)	535:570	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	2	57	theme	modified	248:255	arg1	g-C3N4/SA					303:311	g-C3N4/SA	303:311	g-C3N4/SA	303:311	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	2	57	theme	modified	248:255	arg1	hydrogels					292:300	alginate modified graphitic carbon nitride composite hydrogels	239:300	alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA)	239:312	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	4	58	theme	initial	675:681	arg1	concentration					683:695	initial concentration	675:695	initial concentration	675:695	The effects of different conditions were examined such as pH, initial concentration and contact time.					
31739024	1	59	theme	metal	147:151	arg1	ions					153:156	heavy metal ions	141:156	heavy metal ions	141:156	The removal of heavy metal ions is of great significance to the friendly development of the environment.					
31739024	4	60	theme	contact	701:707	arg1	time					709:712	contact time	701:712	contact time	701:712	The effects of different conditions were examined such as pH, initial concentration and contact time.					
31739024	9	61	from	water	1617:1621	arg1	adsorption					1554:1563	highly effective adsorption	1537:1563	highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water	1537:1621	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	2	62	theme	alginate	239:246	arg1	g-C3N4/SA					303:311	g-C3N4/SA	303:311	g-C3N4/SA	303:311	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	2	62	theme	alginate	239:246	arg1	hydrogels					292:300	alginate modified graphitic carbon nitride composite hydrogels	239:300	alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA)	239:312	Herein, alginate modified graphitic carbon nitride composite hydrogels (g-C3N4/SA) were successfully synthesized through a facile cross-linking polymerization method.					
31739024	1	63	theme	ions	153:156	arg1	removal					130:136	The removal	126:136	The removal of heavy metal ions	126:156	The removal of heavy metal ions is of great significance to the friendly development of the environment.					
31739024	7	64	theme	isotherms	1104:1112	arg1	studies					1114:1120	Adsorption isotherms studies	1093:1120	Adsorption isotherms studies	1093:1120	Adsorption isotherms studies showed that the equilibrium adsorption data fitted well to the Langmuir model and the adsorption kinetic data described well with the pseudo-second-order model according to kinetics studies.					
31739024	3	65	theme	novel	403:407	arg1	hydrogels					419:427	This novel composite hydrogels	398:427	This novel composite hydrogels	398:427	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	3	66	theme	composite	409:417	arg1	hydrogels					419:427	This novel composite hydrogels	398:427	This novel composite hydrogels	398:427	This novel composite hydrogels were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS).					
31739024	5	67	theme	targeted	829:836	arg1	ions					844:847	targeted metal ions	829:847	targeted metal ions	829:847	It demonstrated that this integration process significantly improved the adsorption capacity of each component to targeted metal ions and solved the recycling and separation problem of g-C3N4, which is powdery sorbent and hard to recycle after adsorption.					
31739024	7	68	theme	Langmuir	1185:1192	arg1	model					1194:1198	the Langmuir model	1181:1198	the Langmuir model	1181:1198	Adsorption isotherms studies showed that the equilibrium adsorption data fitted well to the Langmuir model and the adsorption kinetic data described well with the pseudo-second-order model according to kinetics studies.					
31739024	9	69	theme	As-fabricated	1468:1480	arg1	g-C3N4/SA					1482:1490	As-fabricated g-C3N4/SA	1468:1490	As-fabricated g-C3N4/SA	1468:1490	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	9	69	theme	As-fabricated	1468:1480	arg1	sorbent					1525:1531	a potential sorbent	1513:1531	a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water	1513:1621	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	0	70	theme	efficient	67:75	arg1	removal					77:83	efficient removal	67:83	efficient removal of Pb(II), Ni(II) and Cu(II) from water	67:123	Alginate modified graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water.					
31739024	0	71	theme	graphitic	18:26	arg1	hydrogels					53:61	graphitic carbon nitride composite hydrogels	18:61	graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water	18:123	Alginate modified graphitic carbon nitride composite hydrogels for efficient removal of Pb(II), Ni(II) and Cu(II) from water.					
31739024	7	72	theme	adsorption	1150:1159	arg1	data					1161:1164	the equilibrium adsorption data	1134:1164	the equilibrium adsorption data	1134:1164	Adsorption isotherms studies showed that the equilibrium adsorption data fitted well to the Langmuir model and the adsorption kinetic data described well with the pseudo-second-order model according to kinetics studies.					
31739024	5	73	theme	metal	838:842	arg1	ions					844:847	targeted metal ions	829:847	targeted metal ions	829:847	It demonstrated that this integration process significantly improved the adsorption capacity of each component to targeted metal ions and solved the recycling and separation problem of g-C3N4, which is powdery sorbent and hard to recycle after adsorption.					
31739024	9	74	theme	Cu	1587:1588	arg1	ions					1594:1597	Cu(II) ions	1587:1597	Cu(II) ions	1587:1597	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
31739024	9	75	theme	Pb	1568:1569	arg1	adsorption					1554:1563	highly effective adsorption	1537:1563	highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water	1537:1621	As-fabricated g-C3N4/SA can be considered as a potential sorbent for highly effective adsorption of Pb(II), Ni(II) and Cu(II) ions from contaminated water.					
29630958	7	0	theme	isotherm	1196:1203	arg1	model					1205:1209	the Langmuir isotherm model	1183:1209	the Langmuir isotherm model	1183:1209	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	7	0	theme	isotherm	1196:1203	arg1	suitable					1220:1227	suitable	1220:1227	suitable	1220:1227	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	9	1	theme	MB	1626:1627	arg1	efficiency					1637:1646	high MB removal efficiency	1621:1646	high MB removal efficiency	1621:1646	The regeneration experimental results revealed that MSH-AB-W could be recycled more than six cycles with high MB removal efficiency.					
29630958	10	2	theme	Remazol	1841:1847	arg1	Brilliant					1849:1857	Remazol Brilliant Blue	1841:1862	Remazol Brilliant Blue	1841:1862	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	3	theme	binary	1723:1728	arg1	mixture					1730:1736	the binary mixture	1719:1736	the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency	1719:1916	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	1	4	theme	mesoporous	196:205	arg1	hectorite					217:225	mesoporous synthetic hectorite	196:225	mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent	196:398	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	1	4	theme	mesoporous	196:205	arg1	MSH					228:230	MSH	228:230	MSH	228:230	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	6	5	theme	powder	1102:1107	arg1	MSH					1109:1111	powder MSH	1102:1111	powder MSH	1102:1111	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	10	6	theme	Blue	1859:1862	arg1	Brilliant					1849:1857	Remazol Brilliant Blue	1841:1862	Remazol Brilliant Blue	1841:1862	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	11	7	theme	MSH	1923:1925	arg1	beads					1937:1941	The MSH composite beads	1919:1941	The MSH composite beads	1919:1941	The MSH composite beads were found potentially suitable as an efficient, selective and recyclable adsorbent for the removal of MB from the aqueous solutions.					
29630958	1	8	theme	hectorite	217:225	arg1	clay					233:236	mesoporous synthetic hectorite (MSH) clay	196:236	mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent	196:398	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	7	9	theme	composite	1338:1346	arg1	beads					1348:1352	organized hydrogel composite beads	1319:1352	organized hydrogel composite beads	1319:1352	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	7	10	theme	organized	1319:1327	arg1	beads					1348:1352	organized hydrogel composite beads	1319:1352	organized hydrogel composite beads	1319:1352	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	10	11	theme	dye	1755:1757	arg1	Orange					1801:1806	Methyl Orange	1794:1806	Methyl Orange (MO)	1794:1811	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	11	theme	dye	1755:1757	arg1	Brilliant					1849:1857	Remazol Brilliant Blue	1841:1862	Remazol Brilliant Blue	1841:1862	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	11	theme	dye	1755:1757	arg1	MR					1789:1790	Methyl Red (MR)	1777:1791	Methyl Red (MR)	1777:1791	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	11	theme	dye	1755:1757	arg1	solutions					1759:1767	other dye solutions	1749:1767	other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency	1749:1916	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	11	theme	dye	1755:1757	arg1	Yellow					1824:1829	Alizarine Yellow	1814:1829	Alizarine Yellow (AY)	1814:1834	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	8	12	dep	observed	1398:1405	arg1	whereas					1438:1444	whereas	1438:1444	whereas	1438:1444	The pseudo-second-order kinetics model was observed best for MB sorption onto MSH, whereas pseudo-first order useful to describe the kinetic behavior of MSH-AB.					
29630958	5	13	theme	various	891:897	arg1	parameters					916:925	various batch adsorption parameters	891:925	various batch adsorption parameters	891:925	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	10	14	theme	adsorption	1896:1905	arg1	efficiency					1907:1916	the selective adsorption efficiency	1882:1916	the selective adsorption efficiency	1882:1916	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	7	15	theme	Langmuir	1187:1194	arg1	model					1205:1209	the Langmuir isotherm model	1183:1209	the Langmuir isotherm model	1183:1209	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	7	15	theme	Langmuir	1187:1194	arg1	suitable					1220:1227	suitable	1220:1227	suitable	1220:1227	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	10	16	theme	Methyl	1777:1782	arg1	RBB					1865:1867	RBB	1865:1867	RBB	1865:1867	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	16	theme	Methyl	1777:1782	arg1	MR					1789:1790	Methyl Red (MR)	1777:1791	Methyl Red (MR)	1777:1791	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	5	17	theme	dye	852:854	arg1	removal					832:838	the removal	828:838	the removal of cationic dye, methylene blue (MB)	828:875	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	4	18	theme	0.34	708:711	arg1	area					666:669	the high surface area 468	649:673	the high surface area 468	649:673	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	4	18	theme	0.34	708:711	arg1	205m2/g					676:682	205m2/g	676:682	205m2/g	676:682	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	4	18	theme	0.34	708:711	arg1	volume					698:703	the pore volume	689:703	the pore volume of 0.34, 0.29cm3/g respectively	689:735	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	0	19	theme	methylene	85:93	arg1	dye					100:102	methylene blue dye	85:102	methylene blue dye	85:102	Porous synthetic hectorite clay-alginate composite beads for effective adsorption of methylene blue dye from aqueous solution.					
29630958	6	20	theme	adsorption	951:960	arg1	capacity					962:969	The Langmuir monolayer adsorption capacity	928:969	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W	928:991	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	0	21	theme	dye	100:102	arg1	adsorption					71:80	effective adsorption	61:80	effective adsorption of methylene blue dye from aqueous solution	61:124	Porous synthetic hectorite clay-alginate composite beads for effective adsorption of methylene blue dye from aqueous solution.					
29630958	6	22	theme	Langmuir	932:939	arg1	adsorption					951:960	The Langmuir monolayer adsorption	928:960	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W	928:991	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	2	23	theme	detail	405:410	arg1	characterization					412:427	The detail characterization	401:427	The detail characterization of MSH and MSH-AB	401:445	The detail characterization of MSH and MSH-AB were carried out by various physicochemical techniques.					
29630958	0	24	theme	aqueous	109:115	arg1	solution					117:124	aqueous solution	109:124	aqueous solution	109:124	Porous synthetic hectorite clay-alginate composite beads for effective adsorption of methylene blue dye from aqueous solution.					
29630958	9	25	theme	regeneration	1520:1531	arg1	results					1546:1552	The regeneration experimental results	1516:1552	The regeneration experimental results	1516:1552	The regeneration experimental results revealed that MSH-AB-W could be recycled more than six cycles with high MB removal efficiency.					
29630958	5	26	theme	dry	796:798	arg1	forms					804:808	wet (W) and dry (D) forms	784:808	wet (W) and dry (D) forms	784:808	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	11	27	theme	MB	2046:2047	arg1	removal					2035:2041	the removal	2031:2041	the removal of MB from the aqueous solutions	2031:2074	The MSH composite beads were found potentially suitable as an efficient, selective and recyclable adsorbent for the removal of MB from the aqueous solutions.					
29630958	8	28	theme	MSH-AB	1508:1513	arg1	behavior					1496:1503	the kinetic behavior	1484:1503	the kinetic behavior of MSH-AB	1484:1513	The pseudo-second-order kinetics model was observed best for MB sorption onto MSH, whereas pseudo-first order useful to describe the kinetic behavior of MSH-AB.					
29630958	6	29	dep	785.45mgMB/g	1044:1055	arg1	i.e.					1038:1041	i.e.	1038:1041	i.e.	1038:1041	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	5	30	theme	batch	899:903	arg1	parameters					916:925	various batch adsorption parameters	891:925	various batch adsorption parameters	891:925	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	6	31	theme	adsorption	1017:1026	arg1	efficacy					1028:1035	significant high adsorption efficacy	1000:1035	significant high adsorption efficacy (i.e., 785.45mgMB/g)	1000:1056	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	6	31	theme	adsorption	1017:1026	arg1	785.45mgMB/g					1044:1055	785.45mgMB/g	1044:1055	785.45mgMB/g	1044:1055	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	4	32	theme	textural	596:603	arg1	properties					605:614	The textural properties	592:614	The textural properties results of MSH and MSH-AB	592:640	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	10	33	theme	Methyl	1794:1799	arg1	MO					1809:1810	MO	1809:1810	MO	1809:1810	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	33	theme	Methyl	1794:1799	arg1	Orange					1801:1806	Methyl Orange	1794:1806	Methyl Orange (MO)	1794:1811	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	0	34	theme	composite	41:49	arg1	beads					51:55	clay-alginate composite beads	27:55	clay-alginate composite beads	27:55	Porous synthetic hectorite clay-alginate composite beads for effective adsorption of methylene blue dye from aqueous solution.					
29630958	1	35	theme	synthetic	316:324	arg1	MSH-AB					352:357	MSH-AB	352:357	MSH-AB	352:357	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	1	35	theme	synthetic	316:324	arg1	beads					345:349	mesoporous synthetic hectorite-alginate beads	305:349	mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent	305:398	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	6	36	theme	significant	1000:1010	arg1	efficacy					1028:1035	significant high adsorption efficacy	1000:1035	significant high adsorption efficacy (i.e., 785.45mgMB/g)	1000:1056	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	6	36	theme	significant	1000:1010	arg1	785.45mgMB/g					1044:1055	785.45mgMB/g	1044:1055	785.45mgMB/g	1044:1055	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	2	37	theme	various	467:473	arg1	techniques					491:500	various physicochemical techniques	467:500	various physicochemical techniques	467:500	The detail characterization of MSH and MSH-AB were carried out by various physicochemical techniques.					
29630958	8	38	theme	kinetics	1379:1386	arg1	model					1388:1392	The pseudo-second-order kinetics model	1355:1392	The pseudo-second-order kinetics model	1355:1392	The pseudo-second-order kinetics model was observed best for MB sorption onto MSH, whereas pseudo-first order useful to describe the kinetic behavior of MSH-AB.					
29630958	3	39	theme	thermogravimetric	507:523	arg1	study					534:538	The thermogravimetric analysis study	503:538	The thermogravimetric analysis study	503:538	The thermogravimetric analysis study showed better thermal stability results for MSH-AB.					
29630958	1	40	theme	beads	345:349	arg1	preparation					290:300	the preparation	286:300	the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent	286:398	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	10	41	theme	adsorption	1666:1675	arg1	property					1677:1684	the adsorption property	1662:1684	the adsorption property of the MSH-AB-W	1662:1700	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	4	42	theme	MSH-AB	635:640	arg1	results					616:622	The textural properties results	592:622	The textural properties results of MSH and MSH-AB	592:640	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	3	43	theme	better	547:552	arg1	results					572:578	better thermal stability results	547:578	better thermal stability results	547:578	The thermogravimetric analysis study showed better thermal stability results for MSH-AB.					
29630958	5	44	theme	MSH-AB	774:779	arg1	applicability					742:754	The applicability	738:754	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms	738:808	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	10	45	theme	MSH-AB-W	1693:1700	arg1	property					1677:1684	the adsorption property	1662:1684	the adsorption property of the MSH-AB-W	1662:1700	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	9	46	theme	high	1621:1624	arg1	efficiency					1637:1646	high MB removal efficiency	1621:1646	high MB removal efficiency	1621:1646	The regeneration experimental results revealed that MSH-AB-W could be recycled more than six cycles with high MB removal efficiency.					
29630958	10	47	theme	Alizarine	1814:1822	arg1	AY					1832:1833	AY	1832:1833	AY	1832:1833	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	47	theme	Alizarine	1814:1822	arg1	Yellow					1824:1829	Alizarine Yellow	1814:1829	Alizarine Yellow (AY)	1814:1834	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	8	48	dep	useful	1465:1470	arg1	order					1459:1463	pseudo-first order	1446:1463	pseudo-first order	1446:1463	The pseudo-second-order kinetics model was observed best for MB sorption onto MSH, whereas pseudo-first order useful to describe the kinetic behavior of MSH-AB.					
29630958	11	49	theme	composite	1927:1935	arg1	beads					1937:1941	The MSH composite beads	1919:1941	The MSH composite beads	1919:1941	The MSH composite beads were found potentially suitable as an efficient, selective and recyclable adsorbent for the removal of MB from the aqueous solutions.					
29630958	9	50	theme	removal	1629:1635	arg1	efficiency					1637:1646	high MB removal efficiency	1621:1646	high MB removal efficiency	1621:1646	The regeneration experimental results revealed that MSH-AB-W could be recycled more than six cycles with high MB removal efficiency.					
29630958	4	51	theme	MSH	627:629	arg1	results					616:622	The textural properties results	592:622	The textural properties results of MSH and MSH-AB	592:640	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	10	52	theme	other	1749:1753	arg1	Orange					1801:1806	Methyl Orange	1794:1806	Methyl Orange (MO)	1794:1811	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	52	theme	other	1749:1753	arg1	Brilliant					1849:1857	Remazol Brilliant Blue	1841:1862	Remazol Brilliant Blue	1841:1862	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	52	theme	other	1749:1753	arg1	MR					1789:1790	Methyl Red (MR)	1777:1791	Methyl Red (MR)	1777:1791	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	52	theme	other	1749:1753	arg1	solutions					1759:1767	other dye solutions	1749:1767	other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency	1749:1916	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	52	theme	other	1749:1753	arg1	Yellow					1824:1829	Alizarine Yellow	1814:1829	Alizarine Yellow (AY)	1814:1834	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	53	theme	MB	1741:1742	arg1	mixture					1730:1736	the binary mixture	1719:1736	the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency	1719:1916	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	1	54	theme	synthetic	207:215	arg1	hectorite					217:225	mesoporous synthetic hectorite	196:225	mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent	196:398	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	1	54	theme	synthetic	207:215	arg1	MSH					228:230	MSH	228:230	MSH	228:230	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	8	55	theme	MB	1416:1417	arg1	sorption					1419:1426	MB sorption	1416:1426	MB sorption onto MSH	1416:1435	The pseudo-second-order kinetics model was observed best for MB sorption onto MSH, whereas pseudo-first order useful to describe the kinetic behavior of MSH-AB.					
29630958	4	56	theme	surface	658:664	arg1	area					666:669	the high surface area 468	649:673	the high surface area 468	649:673	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	4	56	theme	surface	658:664	arg1	205m2/g					676:682	205m2/g	676:682	205m2/g	676:682	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	7	57	theme	beads	1348:1352	arg1	behavior					1307:1314	the adsorption behavior	1292:1314	the adsorption behavior of organized hydrogel composite beads	1292:1352	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	5	58	from	applicability	742:754	arg1	forms					804:808	wet (W) and dry (D) forms	784:808	wet (W) and dry (D) forms	784:808	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	5	59	theme	blue	867:870	arg1	methylene					857:865	methylene blue	857:870	methylene blue (MB)	857:875	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	5	59	theme	blue	867:870	arg1	MB					873:874	MB	873:874	MB	873:874	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	5	59	theme	blue	867:870	arg1	dye					852:854	cationic dye	843:854	cationic dye	843:854	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	0	60	from	solution	117:124	arg1	adsorption					71:80	effective adsorption	61:80	effective adsorption of methylene blue dye from aqueous solution	61:124	Porous synthetic hectorite clay-alginate composite beads for effective adsorption of methylene blue dye from aqueous solution.					
29630958	7	61	theme	hydrogel	1329:1336	arg1	beads					1348:1352	organized hydrogel composite beads	1319:1352	organized hydrogel composite beads	1319:1352	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	1	62	theme	present	131:137	arg1	study					139:143	The present study	127:143	The present study	127:143	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	8	63	theme	pseudo-first	1446:1457	arg1	order					1459:1463	pseudo-first order	1446:1463	pseudo-first order	1446:1463	The pseudo-second-order kinetics model was observed best for MB sorption onto MSH, whereas pseudo-first order useful to describe the kinetic behavior of MSH-AB.					
29630958	5	64	theme	MSH	766:768	arg1	applicability					742:754	The applicability	738:754	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms	738:808	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	10	65	theme	Red	1784:1786	arg1	RBB					1865:1867	RBB	1865:1867	RBB	1865:1867	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	65	theme	Red	1784:1786	arg1	MR					1789:1790	Methyl Red (MR)	1777:1791	Methyl Red (MR)	1777:1791	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	5	66	theme	cationic	843:850	arg1	methylene					857:865	methylene blue	857:870	methylene blue (MB)	857:875	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	5	66	theme	cationic	843:850	arg1	dye					852:854	cationic dye	843:854	cationic dye	843:854	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	10	67	with	mixture	1730:1736	arg1	Orange					1801:1806	Methyl Orange	1794:1806	Methyl Orange (MO)	1794:1811	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	67	with	mixture	1730:1736	arg1	Brilliant					1849:1857	Remazol Brilliant Blue	1841:1862	Remazol Brilliant Blue	1841:1862	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	67	with	mixture	1730:1736	arg1	MR					1789:1790	Methyl Red (MR)	1777:1791	Methyl Red (MR)	1777:1791	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	67	with	mixture	1730:1736	arg1	solutions					1759:1767	other dye solutions	1749:1767	other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency	1749:1916	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	10	67	with	mixture	1730:1736	arg1	Yellow					1824:1829	Alizarine Yellow	1814:1829	Alizarine Yellow (AY)	1814:1834	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	7	68	theme	adsorption	1296:1305	arg1	behavior					1307:1314	the adsorption behavior	1292:1314	the adsorption behavior of organized hydrogel composite beads	1292:1352	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	4	69	theme	pore	693:696	arg1	volume					698:703	the pore volume	689:703	the pore volume of 0.34, 0.29cm3/g respectively	689:735	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	10	70	theme	selective	1886:1894	arg1	efficiency					1907:1916	the selective adsorption efficiency	1882:1916	the selective adsorption efficiency	1882:1916	Furthermore, the adsorption property of the MSH-AB-W was examined for the binary mixture of MB with other dye solutions such as Methyl Red (MR), Methyl Orange (MO), Alizarine Yellow (AY), and Remazol Brilliant Blue (RBB) to evaluate the selective adsorption efficiency.					
29630958	0	71	theme	blue	95:98	arg1	dye					100:102	methylene blue dye	85:102	methylene blue dye	85:102	Porous synthetic hectorite clay-alginate composite beads for effective adsorption of methylene blue dye from aqueous solution.					
29630958	7	72	theme	isotherm	1154:1161	arg1	studies					1163:1169	The adsorption isotherm studies	1139:1169	The adsorption isotherm studies	1139:1169	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	5	73	theme	adsorption	905:914	arg1	parameters					916:925	various batch adsorption parameters	891:925	various batch adsorption parameters	891:925	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	6	74	theme	monolayer	941:949	arg1	adsorption					951:960	The Langmuir monolayer adsorption	928:960	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W	928:991	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	7	75	theme	adsorption	1143:1152	arg1	studies					1163:1169	The adsorption isotherm studies	1139:1169	The adsorption isotherm studies	1139:1169	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	8	76	theme	kinetic	1488:1494	arg1	behavior					1496:1503	the kinetic behavior	1484:1503	the kinetic behavior of MSH-AB	1484:1513	The pseudo-second-order kinetics model was observed best for MB sorption onto MSH, whereas pseudo-first order useful to describe the kinetic behavior of MSH-AB.					
29630958	6	77	dep	showed	993:998	arg1	compared					1058:1065	compared	1058:1065	showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g)	993:1136	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	1	78	theme	cross-linking	380:392	arg1	agent					394:398	a cross-linking agent	378:398	a cross-linking agent	378:398	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	1	78	theme	cross-linking	380:392	arg1	Ca2+					366:369	Ca2+	366:369	Ca2+	366:369	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	9	79	theme	experimental	1533:1544	arg1	results					1546:1552	The regeneration experimental results	1516:1552	The regeneration experimental results	1516:1552	The regeneration experimental results revealed that MSH-AB-W could be recycled more than six cycles with high MB removal efficiency.					
29630958	11	80	theme	aqueous	2058:2064	arg1	solutions					2066:2074	the aqueous solutions	2054:2074	the aqueous solutions	2054:2074	The MSH composite beads were found potentially suitable as an efficient, selective and recyclable adsorbent for the removal of MB from the aqueous solutions.					
29630958	1	81	dep	preparation	160:170	arg1	the					156:158	the	156:158	the	156:158	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	0	82	theme	clay-alginate	27:39	arg1	beads					51:55	clay-alginate composite beads	27:55	clay-alginate composite beads	27:55	Porous synthetic hectorite clay-alginate composite beads for effective adsorption of methylene blue dye from aqueous solution.					
29630958	3	83	dep	better	547:552	arg1	stability					562:570	thermal stability	554:570	thermal stability	554:570	The thermogravimetric analysis study showed better thermal stability results for MSH-AB.					
29630958	7	84	theme	Freundlich	1250:1259	arg1	model					1261:1265	the Freundlich model	1246:1265	the Freundlich model	1246:1265	The adsorption isotherm studies showed that the Langmuir isotherm model was best suitable for MSH, whereas the Freundlich model was utilised to describe the adsorption behavior of organized hydrogel composite beads.					
29630958	2	85	theme	MSH-AB	440:445	arg1	characterization					412:427	The detail characterization	401:427	The detail characterization of MSH and MSH-AB	401:445	The detail characterization of MSH and MSH-AB were carried out by various physicochemical techniques.					
29630958	4	86	theme	properties	605:614	arg1	results					616:622	The textural properties results	592:622	The textural properties results of MSH and MSH-AB	592:640	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	2	87	theme	MSH	432:434	arg1	characterization					412:427	The detail characterization	401:427	The detail characterization of MSH and MSH-AB	401:445	The detail characterization of MSH and MSH-AB were carried out by various physicochemical techniques.					
29630958	5	88	theme	wet	784:786	arg1	forms					804:808	wet (W) and dry (D) forms	784:808	wet (W) and dry (D) forms	784:808	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	1	89	theme	mesoporous	305:314	arg1	MSH-AB					352:357	MSH-AB	352:357	MSH-AB	352:357	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	1	89	theme	mesoporous	305:314	arg1	beads					345:349	mesoporous synthetic hectorite-alginate beads	305:349	mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent	305:398	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	6	90	theme	high	1012:1015	arg1	efficacy					1028:1035	significant high adsorption efficacy	1000:1035	significant high adsorption efficacy (i.e., 785.45mgMB/g)	1000:1056	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	6	90	theme	high	1012:1015	arg1	785.45mgMB/g					1044:1055	785.45mgMB/g	1044:1055	785.45mgMB/g	1044:1055	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	2	91	theme	physicochemical	475:489	arg1	techniques					491:500	various physicochemical techniques	467:500	various physicochemical techniques	467:500	The detail characterization of MSH and MSH-AB were carried out by various physicochemical techniques.					
29630958	5	92	theme	powder	759:764	arg1	MSH					766:768	powder MSH	759:768	powder MSH	759:768	The applicability of powder MSH and MSH-AB in wet (W) and dry (D) forms were assessed for the removal of cationic dye, methylene blue (MB) by optimizing various batch adsorption parameters.					
29630958	0	93	theme	effective	61:69	arg1	adsorption					71:80	effective adsorption	61:80	effective adsorption of methylene blue dye from aqueous solution	61:124	Porous synthetic hectorite clay-alginate composite beads for effective adsorption of methylene blue dye from aqueous solution.					
29630958	1	94	theme	hectorite-alginate	326:343	arg1	MSH-AB					352:357	MSH-AB	352:357	MSH-AB	352:357	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	1	94	theme	hectorite-alginate	326:343	arg1	beads					345:349	mesoporous synthetic hectorite-alginate beads	305:349	mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent	305:398	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	8	95	theme	pseudo-second-order	1359:1377	arg1	model					1388:1392	The pseudo-second-order kinetics model	1355:1392	The pseudo-second-order kinetics model	1355:1392	The pseudo-second-order kinetics model was observed best for MB sorption onto MSH, whereas pseudo-first order useful to describe the kinetic behavior of MSH-AB.					
29630958	3	96	theme	analysis	525:532	arg1	study					534:538	The thermogravimetric analysis study	503:538	The thermogravimetric analysis study	503:538	The thermogravimetric analysis study showed better thermal stability results for MSH-AB.					
29630958	6	97	dep	MSH-AB-D	1074:1081	arg1	materials					1113:1121	materials	1113:1121	materials	1113:1121	The Langmuir monolayer adsorption capacity obtained for MSH-AB-W showed significant high adsorption efficacy (i.e., 785.45mgMB/g) compared to the MSH-AB-D (357.14mgMB/g) and powder MSH materials (196.00mgMB/g).					
29630958	1	98	theme	clay	233:236	arg1	characterization					176:191	characterization	176:191	characterization	176:191	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	1	98	theme	clay	233:236	arg1	preparation					160:170	preparation	160:170	preparation	160:170	The present study deals with the preparation and characterization of mesoporous synthetic hectorite (MSH) clay which further encapsulated with Na-alginate for the preparation of mesoporous synthetic hectorite-alginate beads (MSH-AB) where Ca2+ act as a cross-linking agent.					
29630958	4	99	theme	high	653:656	arg1	area					666:669	the high surface area 468	649:673	the high surface area 468	649:673	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	4	99	theme	high	653:656	arg1	205m2/g					676:682	205m2/g	676:682	205m2/g	676:682	The textural properties results of MSH and MSH-AB showed the high surface area 468, 205m2/g, and the pore volume of 0.34, 0.29cm3/g respectively.					
29630958	11	100	from	solutions	2066:2074	arg1	removal					2035:2041	the removal	2031:2041	the removal of MB from the aqueous solutions	2031:2074	The MSH composite beads were found potentially suitable as an efficient, selective and recyclable adsorbent for the removal of MB from the aqueous solutions.					
29630958	3	101	theme	thermal	554:560	arg1	stability					562:570	thermal stability	554:570	thermal stability	554:570	The thermogravimetric analysis study showed better thermal stability results for MSH-AB.					
31260776	0	0	theme	nanofiber/Fe	64:75	arg1	complex					82:88	nanofiber/Fe(III) complex	64:88	nanofiber/Fe(III) complex	64:88	Effective dye adsorption behavior of poly(vinyl alcohol)/chitin nanofiber/Fe(III) complex.					
31260776	8	1	theme	Freundlich	1112:1121	arg1	model					1132:1136	Freundlich isotherm model	1112:1136	Freundlich isotherm model	1112:1136	The adsorption data were better fitted with the pseudo-second-order kinetics and Freundlich isotherm model.					
31260776	4	2	theme	solution	590:597	arg1	pH					599:600	solution pH	590:600	solution pH	590:600	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	12	3	theme	developed	1506:1514	arg1	bionanocomposites					1516:1532	the developed bionanocomposites	1502:1532	the developed bionanocomposites	1502:1532	The obtained results revealed that the developed bionanocomposites could be considered as promising recyclable adsorbents for wastewater treatment.					
31260776	12	3	theme	developed	1506:1514	arg1	adsorbents					1578:1587	promising recyclable adsorbents	1557:1587	promising recyclable adsorbents for wastewater treatment	1557:1612	The obtained results revealed that the developed bionanocomposites could be considered as promising recyclable adsorbents for wastewater treatment.					
31260776	2	4	theme	EDX	393:395	arg1	analysis					398:405	energy dispersive X-ray (EDX) analysis	368:405	energy dispersive X-ray (EDX) analysis	368:405	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	7	5	theme	dye	978:980	arg1	process					993:999	dye adsorption process	978:999	dye adsorption process	978:999	Kinetics, isotherms and thermodynamics of dye adsorption process were also thoroughly studied.					
31260776	5	6	theme	PVA/Fe	762:767	arg1	adsorbents					774:783	PVA/Fe(III) adsorbents	762:783	PVA/Fe(III) adsorbents	762:783	With loading of chitin NFs, the dye removal efficiency of PVA/Fe(III) adsorbents was noticeable increased.					
31260776	1	7	theme	crosslinked	107:117	arg1	poly					119:122	A novel Fe(III) crosslinked poly	91:122	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	1	7	theme	crosslinked	107:117	arg1	alcohol					131:137	vinyl alcohol	125:137	vinyl alcohol	125:137	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	1	7	theme	crosslinked	107:117	arg1	PVA					141:143	PVA	141:143	PVA	141:143	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	2	8	theme	infrared	274:281	arg1	spectroscopy					283:294	Fourier-transform infrared spectroscopy	256:294	Fourier-transform infrared spectroscopy (FTIR)	256:301	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	2	8	theme	infrared	274:281	arg1	FTIR					297:300	FTIR	297:300	FTIR	297:300	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	9	9	theme	adsorbent	1162:1170	arg1	NFs-Fe					1150:1155	PVA-chitin NFs-Fe(III) adsorbent	1139:1170	PVA-chitin NFs-Fe(III) adsorbent	1139:1170	PVA-chitin NFs-Fe(III) adsorbent exhibited a high maximum adsorption capacity of 810.4 mg/g for MO adsorption.					
31260776	3	10	from	effects	412:418	arg1	efficiency					500:509	the dye removal efficiency	484:509	the dye removal efficiency of adsorbents	484:523	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	4	11	theme	dye	628:630	arg1	concentration					632:644	initial dye concentration	620:644	initial dye concentration	620:644	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	1	12	theme	dye	192:194	arg1	adsorption					196:205	dye adsorption	192:205	dye adsorption from water	192:216	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	4	13	theme	initial	620:626	arg1	concentration					632:644	initial dye concentration	620:644	initial dye concentration	620:644	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	6	14	from	decline	876:882	arg1	solution					896:903	alkaline solution	887:903	alkaline solution	887:903	In addition, the dye removal efficiency did not show significant decline in alkaline solution in the presence of chitin NFs.					
31260776	6	14	from	decline	876:882	arg1	presence					912:919	the presence	908:919	the presence of chitin NFs	908:933	In addition, the dye removal efficiency did not show significant decline in alkaline solution in the presence of chitin NFs.					
31260776	3	15	theme	content	431:437	arg1	effects					412:418	The effects	408:418	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents	408:523	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	2	16	theme	Fourier-transform	256:272	arg1	spectroscopy					283:294	Fourier-transform infrared spectroscopy	256:294	Fourier-transform infrared spectroscopy (FTIR)	256:301	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	2	16	theme	Fourier-transform	256:272	arg1	FTIR					297:300	FTIR	297:300	FTIR	297:300	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	6	17	theme	NFs	931:933	arg1	presence					912:919	the presence	908:919	the presence of chitin NFs	908:933	In addition, the dye removal efficiency did not show significant decline in alkaline solution in the presence of chitin NFs.					
31260776	4	18	theme	maximum	665:671	arg1	adsorption					692:701	maximum methyl orange (MO) adsorption	665:701	maximum methyl orange (MO) adsorption	665:701	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	4	19	theme	influencing	559:569	arg1	parameters					571:580	The adsorption influencing parameters	544:580	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration	544:644	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	4	19	theme	influencing	559:569	arg1	concentration					632:644	initial dye concentration	620:644	initial dye concentration	620:644	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	4	19	theme	influencing	559:569	arg1	pH					599:600	solution pH	590:600	solution pH	590:600	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	4	19	theme	influencing	559:569	arg1	time					611:614	contact time	603:614	contact time	603:614	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	1	20	theme	poly	119:122	arg1	composites					163:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	6	21	theme	removal	832:838	arg1	efficiency					840:849	the dye removal efficiency	824:849	the dye removal efficiency	824:849	In addition, the dye removal efficiency did not show significant decline in alkaline solution in the presence of chitin NFs.					
31260776	9	22	theme	high	1184:1187	arg1	capacity					1208:1215	a high maximum adsorption capacity	1182:1215	a high maximum adsorption capacity of 810.4 mg/g for MO adsorption	1182:1247	PVA-chitin NFs-Fe(III) adsorbent exhibited a high maximum adsorption capacity of 810.4 mg/g for MO adsorption.					
31260776	7	23	theme	adsorption	982:991	arg1	process					993:999	dye adsorption process	978:999	dye adsorption process	978:999	Kinetics, isotherms and thermodynamics of dye adsorption process were also thoroughly studied.					
31260776	8	24	theme	adsorption	1035:1044	arg1	data					1046:1049	The adsorption data	1031:1049	The adsorption data	1031:1049	The adsorption data were better fitted with the pseudo-second-order kinetics and Freundlich isotherm model.					
31260776	10	25	theme	adsorption	1299:1308	arg1	efficiency					1310:1319	the adsorption efficiency	1295:1319	the adsorption efficiency	1295:1319	Furthermore, reuse experiments revealed that the adsorption efficiency remained almost constant during five cycles of adsorption/desorption.					
31260776	6	26	theme	chitin	924:929	arg1	NFs					931:933	chitin NFs	924:933	chitin NFs	924:933	In addition, the dye removal efficiency did not show significant decline in alkaline solution in the presence of chitin NFs.					
31260776	0	27	theme	dye	10:12	arg1	behavior					25:32	Effective dye adsorption behavior	0:32	Effective dye adsorption behavior of poly(vinyl alcohol)	0:55	Effective dye adsorption behavior of poly(vinyl alcohol)/chitin nanofiber/Fe(III) complex.					
31260776	9	28	theme	maximum	1189:1195	arg1	capacity					1208:1215	a high maximum adsorption capacity	1182:1215	a high maximum adsorption capacity of 810.4 mg/g for MO adsorption	1182:1247	PVA-chitin NFs-Fe(III) adsorbent exhibited a high maximum adsorption capacity of 810.4 mg/g for MO adsorption.					
31260776	6	29	theme	dye	828:830	arg1	efficiency					840:849	the dye removal efficiency	824:849	the dye removal efficiency	824:849	In addition, the dye removal efficiency did not show significant decline in alkaline solution in the presence of chitin NFs.					
31260776	3	30	theme	nanofibers	450:459	arg1	effects					412:418	The effects	408:418	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents	408:523	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	12	31	theme	wastewater	1593:1602	arg1	treatment					1604:1612	wastewater treatment	1593:1612	wastewater treatment	1593:1612	The obtained results revealed that the developed bionanocomposites could be considered as promising recyclable adsorbents for wastewater treatment.					
31260776	1	32	theme	vinyl	125:129	arg1	poly					119:122	A novel Fe(III) crosslinked poly	91:122	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	1	32	theme	vinyl	125:129	arg1	alcohol					131:137	vinyl alcohol	125:137	vinyl alcohol	125:137	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	0	33	theme	Effective	0:8	arg1	behavior					25:32	Effective dye adsorption behavior	0:32	Effective dye adsorption behavior of poly(vinyl alcohol)	0:55	Effective dye adsorption behavior of poly(vinyl alcohol)/chitin nanofiber/Fe(III) complex.					
31260776	3	34	dep	content	431:437	arg1	incorporation					467:479	incorporation	467:479	incorporation	467:479	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	1	35	from	water	212:216	arg1	adsorption					196:205	dye adsorption	192:205	dye adsorption from water	192:216	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	4	36	theme	adsorption	548:557	arg1	parameters					571:580	The adsorption influencing parameters	544:580	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration	544:644	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	4	36	theme	adsorption	548:557	arg1	concentration					632:644	initial dye concentration	620:644	initial dye concentration	620:644	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	4	36	theme	adsorption	548:557	arg1	pH					599:600	solution pH	590:600	solution pH	590:600	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	4	36	theme	adsorption	548:557	arg1	time					611:614	contact time	603:614	contact time	603:614	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	10	37	theme	adsorption/desorption	1368:1388	arg1	cycles					1358:1363	five cycles	1353:1363	five cycles of adsorption/desorption	1353:1388	Furthermore, reuse experiments revealed that the adsorption efficiency remained almost constant during five cycles of adsorption/desorption.					
31260776	11	38	theme	dye	1427:1429	arg1	adsorption-desorption					1431:1451	dye adsorption-desorption	1427:1451	dye adsorption-desorption	1427:1451	At last, the possible mechanism for dye adsorption-desorption was proposed.					
31260776	5	39	dep	increased	800:808	arg1	noticeable					789:798	noticeable	789:798	noticeable	789:798	With loading of chitin NFs, the dye removal efficiency of PVA/Fe(III) adsorbents was noticeable increased.					
31260776	4	40	theme	contact	603:609	arg1	time					611:614	contact time	603:614	contact time	603:614	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	0	41	theme	adsorption	14:23	arg1	behavior					25:32	Effective dye adsorption behavior	0:32	Effective dye adsorption behavior of poly(vinyl alcohol)	0:55	Effective dye adsorption behavior of poly(vinyl alcohol)/chitin nanofiber/Fe(III) complex.					
31260776	1	42	link	crosslinked	107:117	arg1	poly					119:122	A novel Fe(III) crosslinked poly	91:122	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	1	42	link	crosslinked	107:117	arg1	alcohol					131:137	vinyl alcohol	125:137	vinyl alcohol	125:137	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	1	42	link	crosslinked	107:117	arg1	PVA					141:143	PVA	141:143	PVA	141:143	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	3	43	theme	chitin	443:448	arg1	NFs					462:464	NFs	462:464	NFs	462:464	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	3	43	theme	chitin	443:448	arg1	nanofibers					450:459	chitin nanofibers	443:459	chitin nanofibers (NFs)	443:465	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	12	44	theme	recyclable	1567:1576	arg1	bionanocomposites					1516:1532	the developed bionanocomposites	1502:1532	the developed bionanocomposites	1502:1532	The obtained results revealed that the developed bionanocomposites could be considered as promising recyclable adsorbents for wastewater treatment.					
31260776	12	44	theme	recyclable	1567:1576	arg1	adsorbents					1578:1587	promising recyclable adsorbents	1557:1587	promising recyclable adsorbents for wastewater treatment	1557:1612	The obtained results revealed that the developed bionanocomposites could be considered as promising recyclable adsorbents for wastewater treatment.					
31260776	2	45	theme	electron	338:345	arg1	SEM					359:361	SEM	359:361	SEM	359:361	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	2	45	theme	electron	338:345	arg1	microscope					347:356	scanning electron microscope	329:356	scanning electron microscope (SEM)	329:362	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	0	46	theme	poly	37:40	arg1	behavior					25:32	Effective dye adsorption behavior	0:32	Effective dye adsorption behavior of poly(vinyl alcohol)	0:55	Effective dye adsorption behavior of poly(vinyl alcohol)/chitin nanofiber/Fe(III) complex.					
31260776	9	47	theme	810.4 mg/g	1220:1229	arg1	capacity					1208:1215	a high maximum adsorption capacity	1182:1215	a high maximum adsorption capacity of 810.4 mg/g for MO adsorption	1182:1247	PVA-chitin NFs-Fe(III) adsorbent exhibited a high maximum adsorption capacity of 810.4 mg/g for MO adsorption.					
31260776	5	48	theme	chitin	720:725	arg1	NFs					727:729	chitin NFs	720:729	chitin NFs	720:729	With loading of chitin NFs, the dye removal efficiency of PVA/Fe(III) adsorbents was noticeable increased.					
31260776	5	49	theme	removal	740:746	arg1	efficiency					748:757	the dye removal efficiency	732:757	the dye removal efficiency of PVA/Fe(III) adsorbents	732:783	With loading of chitin NFs, the dye removal efficiency of PVA/Fe(III) adsorbents was noticeable increased.					
31260776	2	50	theme	scanning	329:336	arg1	SEM					359:361	SEM	359:361	SEM	359:361	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	2	50	theme	scanning	329:336	arg1	microscope					347:356	scanning electron microscope	329:356	scanning electron microscope (SEM)	329:362	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	2	51	theme	X-ray	386:390	arg1	analysis					398:405	energy dispersive X-ray (EDX) analysis	368:405	energy dispersive X-ray (EDX) analysis	368:405	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	9	52	theme	adsorption	1197:1206	arg1	capacity					1208:1215	a high maximum adsorption capacity	1182:1215	a high maximum adsorption capacity of 810.4 mg/g for MO adsorption	1182:1247	PVA-chitin NFs-Fe(III) adsorbent exhibited a high maximum adsorption capacity of 810.4 mg/g for MO adsorption.					
31260776	5	53	theme	dye	736:738	arg1	efficiency					748:757	the dye removal efficiency	732:757	the dye removal efficiency of PVA/Fe(III) adsorbents	732:783	With loading of chitin NFs, the dye removal efficiency of PVA/Fe(III) adsorbents was noticeable increased.					
31260776	12	54	theme	obtained	1471:1478	arg1	results					1480:1486	The obtained results	1467:1486	The obtained results	1467:1486	The obtained results revealed that the developed bionanocomposites could be considered as promising recyclable adsorbents for wastewater treatment.					
31260776	5	55	theme	NFs	727:729	arg1	loading					709:715	loading	709:715	loading of chitin NFs	709:729	With loading of chitin NFs, the dye removal efficiency of PVA/Fe(III) adsorbents was noticeable increased.					
31260776	2	56	theme	dispersive	375:384	arg1	analysis					398:405	energy dispersive X-ray (EDX) analysis	368:405	energy dispersive X-ray (EDX) analysis	368:405	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	9	57	theme	MO	1235:1236	arg1	adsorption					1238:1247	MO adsorption	1235:1247	MO adsorption	1235:1247	PVA-chitin NFs-Fe(III) adsorbent exhibited a high maximum adsorption capacity of 810.4 mg/g for MO adsorption.					
31260776	3	58	theme	adsorbents	514:523	arg1	efficiency					500:509	the dye removal efficiency	484:509	the dye removal efficiency of adsorbents	484:523	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	2	59	theme	energy	368:373	arg1	analysis					398:405	energy dispersive X-ray (EDX) analysis	368:405	energy dispersive X-ray (EDX) analysis	368:405	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	10	60	theme	reuse	1263:1267	arg1	experiments					1269:1279	reuse experiments	1263:1279	reuse experiments	1263:1279	Furthermore, reuse experiments revealed that the adsorption efficiency remained almost constant during five cycles of adsorption/desorption.					
31260776	5	61	theme	adsorbents	774:783	arg1	efficiency					748:757	the dye removal efficiency	732:757	the dye removal efficiency of PVA/Fe(III) adsorbents	732:783	With loading of chitin NFs, the dye removal efficiency of PVA/Fe(III) adsorbents was noticeable increased.					
31260776	9	62	dep	NFs-Fe	1150:1155	arg1	III					1157:1159	III	1157:1159	III	1157:1159	PVA-chitin NFs-Fe(III) adsorbent exhibited a high maximum adsorption capacity of 810.4 mg/g for MO adsorption.					
31260776	7	63	theme	process	993:999	arg1	thermodynamics					960:973	thermodynamics	960:973	thermodynamics	960:973	Kinetics, isotherms and thermodynamics of dye adsorption process were also thoroughly studied.					
31260776	7	63	theme	process	993:999	arg1	isotherms					946:954	isotherms	946:954	isotherms	946:954	Kinetics, isotherms and thermodynamics of dye adsorption process were also thoroughly studied.					
31260776	7	63	theme	process	993:999	arg1	Kinetics					936:943	Kinetics	936:943	Kinetics	936:943	Kinetics, isotherms and thermodynamics of dye adsorption process were also thoroughly studied.					
31260776	4	64	theme	orange	680:685	arg1	adsorption					692:701	maximum methyl orange (MO) adsorption	665:701	maximum methyl orange (MO) adsorption	665:701	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	3	65	theme	removal	492:498	arg1	efficiency					500:509	the dye removal efficiency	484:509	the dye removal efficiency of adsorbents	484:523	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	8	66	theme	isotherm	1123:1130	arg1	model					1132:1136	Freundlich isotherm model	1112:1136	Freundlich isotherm model	1112:1136	The adsorption data were better fitted with the pseudo-second-order kinetics and Freundlich isotherm model.					
31260776	1	67	theme	novel	93:97	arg1	III					102:104	III	102:104	III	102:104	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	1	67	theme	novel	93:97	arg1	Fe					99:100	A novel Fe	91:100	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	1	68	theme	/chitin	145:151	arg1	composites					163:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	9	69	theme	PVA-chitin	1139:1148	arg1	NFs-Fe					1150:1155	PVA-chitin NFs-Fe(III) adsorbent	1139:1170	PVA-chitin NFs-Fe(III) adsorbent	1139:1170	PVA-chitin NFs-Fe(III) adsorbent exhibited a high maximum adsorption capacity of 810.4 mg/g for MO adsorption.					
31260776	4	70	theme	methyl	673:678	arg1	adsorption					692:701	maximum methyl orange (MO) adsorption	665:701	maximum methyl orange (MO) adsorption	665:701	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	12	71	theme	promising	1557:1565	arg1	bionanocomposites					1516:1532	the developed bionanocomposites	1502:1532	the developed bionanocomposites	1502:1532	The obtained results revealed that the developed bionanocomposites could be considered as promising recyclable adsorbents for wastewater treatment.					
31260776	12	71	theme	promising	1557:1565	arg1	adsorbents					1578:1587	promising recyclable adsorbents	1557:1587	promising recyclable adsorbents for wastewater treatment	1557:1612	The obtained results revealed that the developed bionanocomposites could be considered as promising recyclable adsorbents for wastewater treatment.					
31260776	1	72	theme	Fe	99:100	arg1	poly					119:122	A novel Fe(III) crosslinked poly	91:122	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	1	72	theme	Fe	99:100	arg1	alcohol					131:137	vinyl alcohol	125:137	vinyl alcohol	125:137	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	1	72	theme	Fe	99:100	arg1	PVA					141:143	PVA	141:143	PVA	141:143	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	8	73	theme	pseudo-second-order	1079:1097	arg1	kinetics					1099:1106	the pseudo-second-order kinetics	1075:1106	the pseudo-second-order kinetics	1075:1106	The adsorption data were better fitted with the pseudo-second-order kinetics and Freundlich isotherm model.					
31260776	1	74	theme	nanofiber	153:161	arg1	composites					163:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites	91:172	A novel Fe(III) crosslinked poly (vinyl alcohol) (PVA)/chitin nanofiber composites were prepared for dye adsorption from water.					
31260776	6	75	theme	alkaline	887:894	arg1	solution					896:903	alkaline solution	887:903	alkaline solution	887:903	In addition, the dye removal efficiency did not show significant decline in alkaline solution in the presence of chitin NFs.					
31260776	4	76	theme	MO	688:689	arg1	adsorption					692:701	maximum methyl orange (MO) adsorption	665:701	maximum methyl orange (MO) adsorption	665:701	The adsorption influencing parameters such as solution pH, contact time and initial dye concentration were optimized for maximum methyl orange (MO) adsorption.					
31260776	2	77	theme	X-ray	304:308	arg1	XRD					323:325	XRD	323:325	XRD	323:325	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	2	77	theme	X-ray	304:308	arg1	diffraction					310:320	X-ray diffraction	304:320	X-ray diffraction (XRD)	304:326	The adsorbents were characterized by Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analysis.					
31260776	6	78	theme	significant	864:874	arg1	decline					876:882	significant decline	864:882	significant decline in alkaline solution in the presence of chitin NFs	864:933	In addition, the dye removal efficiency did not show significant decline in alkaline solution in the presence of chitin NFs.					
31260776	0	79	theme	vinyl	42:46	arg1	poly					37:40	poly	37:40	poly(vinyl alcohol)	37:55	Effective dye adsorption behavior of poly(vinyl alcohol)/chitin nanofiber/Fe(III) complex.					
31260776	0	79	theme	vinyl	42:46	arg1	alcohol					48:54	vinyl alcohol	42:54	vinyl alcohol	42:54	Effective dye adsorption behavior of poly(vinyl alcohol)/chitin nanofiber/Fe(III) complex.					
31260776	3	80	theme	dye	488:490	arg1	efficiency					500:509	the dye removal efficiency	484:509	the dye removal efficiency of adsorbents	484:523	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	3	81	theme	Fe	423:424	arg1	content					431:437	Fe(III) content	423:437	Fe(III) content	423:437	The effects of Fe(III) content and chitin nanofibers (NFs) incorporation on the dye removal efficiency of adsorbents were investigated.					
31260776	11	82	theme	possible	1404:1411	arg1	mechanism					1413:1421	the possible mechanism	1400:1421	the possible mechanism for dye adsorption-desorption	1400:1451	At last, the possible mechanism for dye adsorption-desorption was proposed.					
30930265	6	0	theme	minimum	993:999	arg1	values					1032:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	9	1	theme	higher	1278:1283	arg1	activities					1285:1294	comparable or even higher activities	1259:1294	comparable or even higher activities	1259:1294	The hydrogel H4 and H1/MWCNT composites have comparable or even higher activities than the reference bactericides or fungicides against some of tested microbes.					
30930265	1	2	theme	Novel	196:200	arg1	isothiocyanate					224:237	Novel trimellitic anhydride isothiocyanate	196:237	Novel trimellitic anhydride isothiocyanate	196:237	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	6	3	theme	lower	987:991	arg1	values					1032:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	5	4	theme	cross	646:650	arg1	contents					667:674	their cross linking moiety contents	640:674	their cross linking moiety contents	640:674	Their swell abilities appreciably depend on their cross linking moiety contents and MWCNTs concentration.					
30930265	1	5	theme	trimellitic	202:212	arg1	isothiocyanate					224:237	Novel trimellitic anhydride isothiocyanate	196:237	Novel trimellitic anhydride isothiocyanate	196:237	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	1	6	theme	novel	322:326	arg1	hydrogels					350:358	four novel cross linked chitosan hydrogels	317:358	four novel cross linked chitosan hydrogels H1-H4	317:364	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	0	7	theme	multi-walled	123:134	arg1	nanotubes					143:151	multi-walled carbon nanotubes	123:151	multi-walled carbon nanotubes for enhancement of antimicrobial activity	123:193	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	1	8	dep	hydrogels	350:358	arg1	H1-H4					360:364	H1-H4	360:364	four novel cross linked chitosan hydrogels H1-H4	317:364	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	7	9	theme	antimicrobial	1046:1058	arg1	activities					1060:1069	Their antimicrobial activities	1040:1069	Their antimicrobial activities	1040:1069	Their antimicrobial activities increased with increasing their cross linking moiety contents.					
30930265	1	10	link	linked	334:339	arg1	hydrogels					350:358	four novel cross linked chitosan hydrogels	317:358	four novel cross linked chitosan hydrogels H1-H4	317:364	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	1	11	theme	cross	328:332	arg1	hydrogels					350:358	four novel cross linked chitosan hydrogels	317:358	four novel cross linked chitosan hydrogels H1-H4	317:364	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	9	12	theme	H1/MWCNT	1234:1241	arg1	composites					1243:1252	The hydrogel H4 and H1/MWCNT composites	1214:1252	The hydrogel H4 and H1/MWCNT composites	1214:1252	The hydrogel H4 and H1/MWCNT composites have comparable or even higher activities than the reference bactericides or fungicides against some of tested microbes.					
30930265	5	13	theme	moiety	660:665	arg1	contents					667:674	their cross linking moiety contents	640:674	their cross linking moiety contents	640:674	Their swell abilities appreciably depend on their cross linking moiety contents and MWCNTs concentration.					
30930265	11	14	theme	good	1561:1564	arg1	way					1566:1568	a good way	1559:1568	a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields	1559:1686	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	11	14	theme	good	1561:1564	arg1	It					1553:1554	It	1553:1554	It	1553:1554	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	1	15	theme	linked	334:339	arg1	hydrogels					350:358	four novel cross linked chitosan hydrogels	317:358	four novel cross linked chitosan hydrogels H1-H4	317:364	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	8	16	theme	better	1148:1153	arg1	potency					1155:1161	a better potency	1146:1161	a better potency against Gram-positive than Gram-negative bacteria	1146:1211	They showed a better potency against Gram-positive than Gram-negative bacteria.					
30930265	9	17	theme	reference	1305:1313	arg1	bactericides					1315:1326	the reference bactericides	1301:1326	the reference bactericides	1301:1326	The hydrogel H4 and H1/MWCNT composites have comparable or even higher activities than the reference bactericides or fungicides against some of tested microbes.					
30930265	11	18	theme	biomedical	1670:1679	arg1	fields					1681:1686	biomedical fields	1670:1686	biomedical fields	1670:1686	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	1	19	theme	anhydride	214:222	arg1	isothiocyanate					224:237	Novel trimellitic anhydride isothiocyanate	196:237	Novel trimellitic anhydride isothiocyanate	196:237	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	0	20	theme	carbon	136:141	arg1	nanotubes					143:151	multi-walled carbon nanotubes	123:151	multi-walled carbon nanotubes for enhancement of antimicrobial activity	123:193	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	6	21	theme	zone	962:965	arg1	diameters					967:975	their greater inhibition zone diameters	937:975	their greater inhibition zone diameters	937:975	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	5	22	theme	MWCNTs	680:685	arg1	concentration					687:699	MWCNTs concentration	680:699	MWCNTs concentration	680:699	Their swell abilities appreciably depend on their cross linking moiety contents and MWCNTs concentration.					
30930265	11	23	from	candidates	1656:1665	arg1	fields					1681:1686	biomedical fields	1670:1686	biomedical fields	1670:1686	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	6	24	theme	inhibition	951:960	arg1	diameters					967:975	their greater inhibition zone diameters	937:975	their greater inhibition zone diameters	937:975	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	9	25	contain	have	1254:1257	arg1	composites					1243:1252	The hydrogel H4 and H1/MWCNT composites	1214:1252	The hydrogel H4 and H1/MWCNT composites	1214:1252	The hydrogel H4 and H1/MWCNT composites have comparable or even higher activities than the reference bactericides or fungicides against some of tested microbes.					
30930265	9	25	contain	have	1254:1257	arg2	activities					1285:1294	comparable or even higher activities	1259:1294	comparable or even higher activities	1259:1294	The hydrogel H4 and H1/MWCNT composites have comparable or even higher activities than the reference bactericides or fungicides against some of tested microbes.					
30930265	2	26	theme	nanotube	393:400	arg1	biocomposites					410:422	Three multi-walled carbon nanotube (MWCNT) biocomposites	367:422	Three multi-walled carbon nanotube (MWCNT) biocomposites based on H1	367:434	Three multi-walled carbon nanotube (MWCNT) biocomposites based on H1 were also prepared.					
30930265	3	27	theme	elemental	488:496	arg1	analysis					498:505	elemental analysis	488:505	elemental analysis	488:505	Their structures were proven by elemental analysis, FTIR, XRD, SEM and TEM.					
30930265	1	28	theme	chitosan	341:348	arg1	hydrogels					350:358	four novel cross linked chitosan hydrogels	317:358	four novel cross linked chitosan hydrogels H1-H4	317:364	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	2	29	theme	carbon	386:391	arg1	biocomposites					410:422	Three multi-walled carbon nanotube (MWCNT) biocomposites	367:422	Three multi-walled carbon nanotube (MWCNT) biocomposites based on H1	367:434	Three multi-walled carbon nanotube (MWCNT) biocomposites based on H1 were also prepared.					
30930265	6	30	theme	Candida	839:845	arg1	albicans					847:854	Candida albicans	839:854	Candida albicans	839:854	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	6	30	theme	Candida	839:845	arg1	Bacillis					731:738	Bacillis	731:738	Bacillis	731:738	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	2	31	theme	multi-walled	373:384	arg1	biocomposites					410:422	Three multi-walled carbon nanotube (MWCNT) biocomposites	367:422	Three multi-walled carbon nanotube (MWCNT) biocomposites based on H1	367:434	Three multi-walled carbon nanotube (MWCNT) biocomposites based on H1 were also prepared.					
30930265	6	32	dep	Bacillis	731:738	arg1	subtilis					740:747	Bacillis subtilis	731:747	Bacillis subtilis	731:747	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	10	33	theme	cross	1460:1464	arg1	linker					1466:1471	the incorporated cross linker	1443:1471	the incorporated cross linker	1443:1471	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	6	34	theme	greater	943:949	arg1	diameters					967:975	their greater inhibition zone diameters	937:975	their greater inhibition zone diameters	937:975	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	10	35	theme	functionalized	1418:1431	arg1	linker					1466:1471	the incorporated cross linker	1443:1471	the incorporated cross linker	1443:1471	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	10	35	theme	functionalized	1418:1431	arg1	groups					1433:1438	the functionalized groups	1414:1438	the functionalized groups of the incorporated cross linker	1414:1471	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	9	36	theme	hydrogel	1218:1225	arg1	H4					1227:1228	hydrogel H4	1218:1228	hydrogel H4	1218:1228	The hydrogel H4 and H1/MWCNT composites have comparable or even higher activities than the reference bactericides or fungicides against some of tested microbes.					
30930265	10	37	theme	chitosan	1534:1541	arg1	features					1543:1550	the chitosan features	1530:1550	the chitosan features	1530:1550	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	0	38	theme	trimellitic	40:50	arg1	isothiocyanate-cross					62:81	novel trimellitic anhydride isothiocyanate-cross	34:81	novel trimellitic anhydride isothiocyanate-cross	34:81	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	0	39	theme	antimicrobial	172:184	arg1	activity					186:193	antimicrobial activity	172:193	antimicrobial activity	172:193	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	10	40	from	chitosan	1401:1408	arg1	system					1498:1503	one system	1494:1503	one system	1494:1503	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	9	41	theme	H4	1227:1228	arg1	composites					1243:1252	The hydrogel H4 and H1/MWCNT composites	1214:1252	The hydrogel H4 and H1/MWCNT composites	1214:1252	The hydrogel H4 and H1/MWCNT composites have comparable or even higher activities than the reference bactericides or fungicides against some of tested microbes.					
30930265	0	42	theme	novel	34:38	arg1	isothiocyanate-cross					62:81	novel trimellitic anhydride isothiocyanate-cross	34:81	novel trimellitic anhydride isothiocyanate-cross	34:81	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	8	43	theme	Gram-negative	1190:1202	arg1	bacteria					1204:1211	Gram-negative bacteria	1190:1211	Gram-negative bacteria	1190:1211	They showed a better potency against Gram-positive than Gram-negative bacteria.					
30930265	9	44	theme	tested	1358:1363	arg1	microbes					1365:1372	tested microbes	1358:1372	tested microbes	1358:1372	The hydrogel H4 and H1/MWCNT composites have comparable or even higher activities than the reference bactericides or fungicides against some of tested microbes.					
30930265	1	45	used	utilized	272:279	arg2	isothiocyanate					224:237	Novel trimellitic anhydride isothiocyanate	196:237	Novel trimellitic anhydride isothiocyanate	196:237	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	6	46	theme	concentration	1012:1024	arg1	values					1032:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	0	47	theme	isothiocyanate-cross	62:81	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	0	47	theme	isothiocyanate-cross	62:81	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	4	48	theme	pH-	554:556	arg1	materials					585:593	pH- and temperature-responsive materials	554:593	pH- and temperature-responsive materials	554:593	They were found to be pH- and temperature-responsive materials.					
30930265	11	49	theme	promising	1646:1654	arg1	candidates					1656:1665	promising candidates	1646:1665	promising candidates in biomedical fields	1646:1686	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	11	49	theme	promising	1646:1654	arg1	agents					1618:1623	antimicrobial agents	1604:1623	antimicrobial agents that can be taken as promising candidates in biomedical fields	1604:1686	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	7	50	theme	moiety	1117:1122	arg1	contents					1124:1131	moiety contents	1117:1131	moiety contents	1117:1131	Their antimicrobial activities increased with increasing their cross linking moiety contents.					
30930265	0	51	theme	anhydride	52:60	arg1	isothiocyanate-cross					62:81	novel trimellitic anhydride isothiocyanate-cross	34:81	novel trimellitic anhydride isothiocyanate-cross	34:81	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	0	52	theme	activity	186:193	arg1	enhancement					157:167	enhancement	157:167	enhancement of antimicrobial activity	157:193	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	5	53	theme	swell	602:606	arg1	abilities					608:616	Their swell abilities	596:616	Their swell abilities	596:616	Their swell abilities appreciably depend on their cross linking moiety contents and MWCNTs concentration.					
30930265	4	54	theme	temperature-responsive	562:583	arg1	materials					585:593	pH- and temperature-responsive materials	554:593	pH- and temperature-responsive materials	554:593	They were found to be pH- and temperature-responsive materials.					
30930265	10	55	theme	linker	1466:1471	arg1	linker					1466:1471	the incorporated cross linker	1443:1471	the incorporated cross linker	1443:1471	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	10	55	theme	linker	1466:1471	arg1	groups					1433:1438	the functionalized groups	1414:1438	the functionalized groups of the incorporated cross linker	1414:1471	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	10	55	theme	linker	1466:1471	arg1	MWCNTs					1484:1489	MWCNTs	1484:1489	MWCNTs in one system	1484:1503	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	10	55	theme	linker	1466:1471	arg1	chitosan					1401:1408	chitosan	1401:1408	chitosan	1401:1408	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	10	56	from	groups	1433:1438	arg1	system					1498:1503	one system	1494:1503	one system	1494:1503	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	5	57	theme	linking	652:658	arg1	contents					667:674	their cross linking moiety contents	640:674	their cross linking moiety contents	640:674	Their swell abilities appreciably depend on their cross linking moiety contents and MWCNTs concentration.					
30930265	2	58	theme	MWCNT	403:407	arg1	biocomposites					410:422	Three multi-walled carbon nanotube (MWCNT) biocomposites	367:422	Three multi-walled carbon nanotube (MWCNT) biocomposites based on H1	367:434	Three multi-walled carbon nanotube (MWCNT) biocomposites based on H1 were also prepared.					
30930265	11	59	theme	antimicrobial	1604:1616	arg1	candidates					1656:1665	promising candidates	1646:1665	promising candidates in biomedical fields	1646:1686	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	11	59	theme	antimicrobial	1604:1616	arg1	systems					1593:1599	adequate systems	1584:1599	adequate systems	1584:1599	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	11	59	theme	antimicrobial	1604:1616	arg1	agents					1618:1623	antimicrobial agents	1604:1623	antimicrobial agents that can be taken as promising candidates in biomedical fields	1604:1686	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	1	60	theme	various	284:290	arg1	concentrations					292:305	various concentrations	284:305	various concentrations	284:305	Novel trimellitic anhydride isothiocyanate was successfully synthesized and utilized in various concentrations to obtain four novel cross linked chitosan hydrogels H1-H4.					
30930265	10	61	theme	incorporated	1447:1458	arg1	linker					1466:1471	the incorporated cross linker	1443:1471	the incorporated cross linker	1443:1471	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	9	62	theme	comparable	1259:1268	arg1	activities					1285:1294	comparable or even higher activities	1259:1294	comparable or even higher activities	1259:1294	The hydrogel H4 and H1/MWCNT composites have comparable or even higher activities than the reference bactericides or fungicides against some of tested microbes.					
30930265	11	63	theme	adequate	1584:1591	arg1	systems					1593:1599	adequate systems	1584:1599	adequate systems	1584:1599	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	11	63	theme	adequate	1584:1591	arg1	agents					1618:1623	antimicrobial agents	1604:1623	antimicrobial agents that can be taken as promising candidates in biomedical fields	1604:1686	It is a good way for attaining adequate systems as antimicrobial agents that can be taken as promising candidates in biomedical fields.					
30930265	0	64	theme	chitosan	90:97	arg1	hydrogels					99:107	chitosan hydrogels	90:107	chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity	90:193	Synthesis and characterization of novel trimellitic anhydride isothiocyanate-cross linked chitosan hydrogels modified with multi-walled carbon nanotubes for enhancement of antimicrobial activity.					
30930265	10	65	from	MWCNTs	1484:1489	arg1	system					1498:1503	one system	1494:1503	one system	1494:1503	Thus, combination between chitosan and the functionalized groups of the incorporated cross linker as well as MWCNTs in one system has efficiently improved the chitosan features.					
30930265	6	66	theme	inhibitory	1001:1010	arg1	values					1032:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	6	67	dep	Geotrichum	818:827	arg1	candidum					829:836	Geotrichum candidum	818:836	Geotrichum candidum	818:836	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30930265	6	68	theme	MIC	1027:1029	arg1	values					1032:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	their lower minimum inhibitory concentration (MIC) values	981:1037	They are more potent against Bacillis subtilis, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Geotrichum candidum, Candida albicans, Aspergillus fumigatus, and Syncephalastrum racemosum than chitosan as judged by their greater inhibition zone diameters and their lower minimum inhibitory concentration (MIC) values.					
30706639	8	0	theme	nerve	1315:1319	arg1	regeneration					1287:1298	the regeneration	1283:1298	the regeneration of rat sciatic nerve	1283:1319	Further on, hematoxylin-eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve.					
30706639	7	1	theme	suitable	1073:1080	arg1	properties					1090:1099	suitable elastic properties	1073:1099	suitable elastic properties	1073:1099	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
30706639	9	2	theme	hydrogel	1372:1379	arg1	conduits					1381:1388	the as prepared hydrogel conduits	1356:1388	the as prepared hydrogel conduits	1356:1388	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	9	2	theme	hydrogel	1372:1379	arg1	beneficial					1399:1408	beneficial	1399:1408	beneficial	1399:1408	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	2	3	theme	sciatic	347:353	arg1	defects					361:367	rat sciatic nerve defects	343:367	rat sciatic nerve defects	343:367	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	11	4	dep	Res	1547:1549	arg1	107A					1559:1562	107A	1559:1562	107A	1559:1562	J Biomed Mater Res Part A: 107A: 1273-1283, 2019.					
30706639	11	4	dep	Res	1547:1549	arg1	A					1556:1556	Part A	1551:1556	J Biomed Mater Res Part A: 107A: 1273-1283, 2019.	1532:1580	J Biomed Mater Res Part A: 107A: 1273-1283, 2019.					
30706639	8	5	theme	rat	1303:1305	arg1	nerve					1315:1319	rat sciatic nerve	1303:1319	rat sciatic nerve	1303:1319	Further on, hematoxylin-eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve.					
30706639	7	6	theme	elastic	1082:1088	arg1	properties					1090:1099	suitable elastic properties	1073:1099	suitable elastic properties	1073:1099	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
30706639	2	7	theme	rat	343:345	arg1	defects					361:367	rat sciatic nerve defects	343:367	rat sciatic nerve defects	343:367	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	8	8	theme	immunohistochemistry	1203:1222	arg1	staining					1224:1231	immunohistochemistry staining	1203:1231	immunohistochemistry staining	1203:1231	Further on, hematoxylin-eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve.					
30706639	8	9	dep	Further	1133:1139	arg1	on					1141:1142	on	1141:1142	on	1141:1142	Further on, hematoxylin-eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve.					
30706639	3	10	theme	hydrogel	384:391	arg1	conduit					393:399	The composite hydrogel conduit	370:399	The composite hydrogel conduit	370:399	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	3	11	theme	self-made	552:560	arg1	mold					562:565	a self-made mold	550:565	a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm	550:626	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	7	12	theme	good	1105:1108	arg1	properties					1121:1130	good mechanical properties	1105:1130	good mechanical properties	1105:1130	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
30706639	2	13	theme	hydrogel	276:283	arg1	conduit					285:291	a bioactive composite hydrogel conduit	254:291	a bioactive composite hydrogel conduit	254:291	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	2	13	theme	hydrogel	276:283	arg1	scaffold					316:323	a three-dimensional scaffold	296:323	a three-dimensional scaffold for the repair of rat sciatic nerve defects	296:367	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	3	14	theme	gelatin	481:487	arg1	volumes					438:444	different volumes	428:444	different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS)	428:529	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	3	15	theme	oxide	474:478	arg1	volumes					438:444	different volumes	428:444	different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS)	428:529	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	2	16	theme	composite	266:274	arg1	conduit					285:291	a bioactive composite hydrogel conduit	254:291	a bioactive composite hydrogel conduit	254:291	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	2	16	theme	composite	266:274	arg1	scaffold					316:323	a three-dimensional scaffold	296:323	a three-dimensional scaffold for the repair of rat sciatic nerve defects	296:367	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	11	17	dep	107A	1559:1562	arg1	1273-1283					1565:1573	1273-1283	1565:1573	1273-1283	1565:1573	J Biomed Mater Res Part A: 107A: 1273-1283, 2019.					
30706639	9	18	contain	have	1445:1448	arg1	conduits					1381:1388	the as prepared hydrogel conduits	1356:1388	the as prepared hydrogel conduits	1356:1388	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	9	18	contain	have	1445:1448	arg2	value					1472:1476	potential application value	1450:1476	potential application value	1450:1476	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	9	18	contain	have	1445:1448	arg1	beneficial					1399:1408	beneficial	1399:1408	beneficial	1399:1408	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	5	19	theme	conduit	709:715	arg1	properties					691:700	The properties	687:700	The properties of the conduit	687:715	The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration.					
30706639	8	20	theme	sciatic	1307:1313	arg1	nerve					1315:1319	rat sciatic nerve	1303:1319	rat sciatic nerve	1303:1319	Further on, hematoxylin-eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve.					
30706639	7	21	theme	hydrogel	1052:1059	arg1	conduit					1061:1067	the hydrogel conduit	1048:1067	the hydrogel conduit	1048:1067	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
30706639	11	22	theme	Part	1551:1554	arg1	A					1556:1556	Part A	1551:1556	J Biomed Mater Res Part A: 107A: 1273-1283, 2019.	1532:1580	J Biomed Mater Res Part A: 107A: 1273-1283, 2019.					
30706639	2	23	theme	bioactive	256:264	arg1	conduit					285:291	a bioactive composite hydrogel conduit	254:291	a bioactive composite hydrogel conduit	254:291	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	2	23	theme	bioactive	256:264	arg1	scaffold					316:323	a three-dimensional scaffold	296:323	a three-dimensional scaffold for the repair of rat sciatic nerve defects	296:367	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	1	24	theme	hot	183:185	arg1	topic					187:191	a hot topic	181:191	a hot topic in clinical research	181:212	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	1	24	theme	hot	183:185	arg1	repair					111:116	effective repair	101:116	effective repair	101:116	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	1	24	theme	hot	183:185	arg1	recovery					133:140	functional recovery	122:140	functional recovery	122:140	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	0	25	theme	composite	14:22	arg1	conduit					33:39	PAM/GO/gel/SA composite hydrogel conduit	0:39	PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury.	0:95	PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury.					
30706639	5	26	theme	nerve	894:898	arg1	regeneration					900:911	nerve regeneration	894:911	nerve regeneration	894:911	The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration.					
30706639	5	27	theme	rat	834:836	arg1	nerve					846:850	rat sciatic nerve	834:850	rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration	834:911	The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration.					
30706639	0	28	theme	PAM/GO/gel/SA	0:12	arg1	conduit					33:39	PAM/GO/gel/SA composite hydrogel conduit	0:39	PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury.	0:95	PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury.					
30706639	6	29	theme	analysis	938:945	arg1	results					918:924	The results	914:924	The results of diameter analysis	914:945	The results of diameter analysis indicated that the wall of hydrogel conduit was uniform.					
30706639	8	30	theme	trichrome	1180:1188	arg1	staining					1190:1197	masson trichrome staining	1173:1197	masson trichrome staining	1173:1197	Further on, hematoxylin-eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve.					
30706639	2	31	theme	three-dimensional	298:314	arg1	conduit					285:291	a bioactive composite hydrogel conduit	254:291	a bioactive composite hydrogel conduit	254:291	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	2	31	theme	three-dimensional	298:314	arg1	scaffold					316:323	a three-dimensional scaffold	296:323	a three-dimensional scaffold for the repair of rat sciatic nerve defects	296:367	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	3	32	theme	different	428:436	arg1	volumes					438:444	different volumes	428:444	different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS)	428:529	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	3	33	with	mold	562:565	arg1	diameter					581:588	an inner diameter	572:588	an inner diameter of 2 mm and an outer diameter of 6 mm	572:626	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	5	34	theme	tensile	779:785	arg1	morphology					747:756	morphology	747:756	morphology	747:756	The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration.					
30706639	5	34	theme	tensile	779:785	arg1	test					787:790	tensile test	779:790	tensile test	779:790	The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration.					
30706639	9	35	theme	nerve	1424:1428	arg1	repair					1430:1435	nerve repair	1424:1435	nerve repair	1424:1435	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	5	36	dep	nerve	846:850	arg1	gap					852:854	gap	852:854	gap to evaluate their effect on promoting nerve regeneration	852:911	The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration.					
30706639	1	37	theme	clinical	196:203	arg1	research					205:212	clinical research	196:212	clinical research	196:212	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	1	38	theme	effective	101:109	arg1	topic					187:191	a hot topic	181:191	a hot topic in clinical research	181:212	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	1	38	theme	effective	101:109	arg1	repair					111:116	effective repair	101:116	effective repair	101:116	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	1	38	theme	effective	101:109	arg1	recovery					133:140	functional recovery	122:140	functional recovery	122:140	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	7	39	theme	conduit	1028:1034	arg1	test					1016:1019	The tensile test	1004:1019	The tensile test of the conduit	1004:1034	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
30706639	3	40	theme	alginate	501:508	arg1	volumes					438:444	different volumes	428:444	different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS)	428:529	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	1	41	dep	repair	111:116	arg1	The					97:99	The	97:99	The	97:99	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	3	42	theme	sodium	494:499	arg1	alginate					501:508	sodium alginate	494:508	sodium alginate (PAM/GO/Gel/SA，PGGS)	494:529	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	3	42	theme	sodium	494:499	arg1	PAM/GO/Gel/SA，PGGS					511:528	PAM/GO/Gel/SA，PGGS	511:528	PAM/GO/Gel/SA，PGGS	511:528	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	9	43	theme	nerve	1481:1485	arg1	regeneration					1487:1498	nerve regeneration	1481:1498	nerve regeneration	1481:1498	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	3	44	theme	mm	625:626	arg1	diameter					611:618	an outer diameter	602:618	an outer diameter of 6 mm	602:626	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	3	44	theme	mm	625:626	arg1	2 mm					593:596	2 mm	593:596	2 mm	593:596	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	2	45	theme	study	231:235	arg1	aim					219:221	The aim	215:221	The aim of this study	215:235	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	5	46	theme	sciatic	838:844	arg1	nerve					846:850	rat sciatic nerve	834:850	rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration	834:911	The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration.					
30706639	3	47	theme	polyacrylamide	449:462	arg1	volumes					438:444	different volumes	428:444	different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS)	428:529	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	7	48	theme	mechanical	1110:1119	arg1	properties					1121:1130	good mechanical properties	1105:1130	good mechanical properties	1105:1130	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
30706639	8	49	theme	masson	1173:1178	arg1	staining					1190:1197	masson trichrome staining	1173:1197	masson trichrome staining	1173:1197	Further on, hematoxylin-eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve.					
30706639	3	50	theme	diameter	611:618	arg1	diameter					581:588	an inner diameter	572:588	an inner diameter of 2 mm and an outer diameter of 6 mm	572:626	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	10	51	dep	©	1501:1501	arg1	Inc.					1527:1530	Inc.	1527:1530	Inc.	1527:1530	© 2019 Wiley Periodicals, Inc.					
30706639	0	52	with	conduit	33:39	arg1	bioactivity					46:56	bioactivity	46:56	bioactivity	46:56	PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury.					
30706639	8	53	theme	hematoxylin-eosin	1145:1161	arg1	staining					1163:1170	hematoxylin-eosin staining	1145:1170	hematoxylin-eosin staining	1145:1170	Further on, hematoxylin-eosin staining, masson trichrome staining and immunohistochemistry staining showed that the conduit could effectively promote the regeneration of rat sciatic nerve.					
30706639	5	54	theme	swelling	759:766	arg1	morphology					747:756	morphology	747:756	morphology	747:756	The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration.					
30706639	5	54	theme	swelling	759:766	arg1	behaviors					768:776	swelling behaviors	759:776	swelling behaviors	759:776	The properties of the conduit were firstly characterized by morphology, swelling behaviors, tensile test and then the conduits were implanted into rat sciatic nerve gap to evaluate their effect on promoting nerve regeneration.					
30706639	6	55	theme	diameter	929:936	arg1	analysis					938:945	diameter analysis	929:945	diameter analysis	929:945	The results of diameter analysis indicated that the wall of hydrogel conduit was uniform.					
30706639	9	56	theme	application	1460:1470	arg1	value					1472:1476	potential application value	1450:1476	potential application value	1450:1476	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	1	57	theme	functional	122:131	arg1	topic					187:191	a hot topic	181:191	a hot topic in clinical research	181:212	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	1	57	theme	functional	122:131	arg1	repair					111:116	effective repair	101:116	effective repair	101:116	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	1	57	theme	functional	122:131	arg1	recovery					133:140	functional recovery	122:140	functional recovery	122:140	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	7	58	theme	tensile	1008:1014	arg1	test					1016:1019	The tensile test	1004:1019	The tensile test of the conduit	1004:1034	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
30706639	3	59	theme	inner	575:579	arg1	diameter					581:588	an inner diameter	572:588	an inner diameter of 2 mm and an outer diameter of 6 mm	572:626	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	9	60	theme	potential	1450:1458	arg1	application					1460:1470	potential application	1450:1470	potential application value	1450:1476	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	4	61	theme	YIGSR	672:676	arg1	peptide					678:684	YIGSR peptide	672:684	YIGSR peptide	672:684	Then the conduit was further modified with YIGSR peptide.					
30706639	4	62	mod	modified	658:665	arg3	peptide					678:684	YIGSR peptide	672:684	YIGSR peptide	672:684	Then the conduit was further modified with YIGSR peptide.					
30706639	4	62	mod	modified	658:665	arg1	conduit					638:644	Then the conduit	629:644	Then the conduit	629:644	Then the conduit was further modified with YIGSR peptide.					
30706639	1	63	theme	nerve	159:163	arg1	injury					165:170	peripheral nerve injury	148:170	peripheral nerve injury	148:170	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	9	64	theme	prepared	1363:1370	arg1	conduits					1381:1388	the as prepared hydrogel conduits	1356:1388	the as prepared hydrogel conduits	1356:1388	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	9	64	theme	prepared	1363:1370	arg1	beneficial					1399:1408	beneficial	1399:1408	beneficial	1399:1408	Thus, the study demonstrates that the as prepared hydrogel conduits here are beneficial for promoting nerve repair and may have potential application value in nerve regeneration.					
30706639	3	65	theme	outer	605:609	arg1	diameter					611:618	an outer diameter	602:618	an outer diameter of 6 mm	602:626	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	1	66	from	topic	187:191	arg1	research					205:212	clinical research	196:212	clinical research	196:212	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	1	67	theme	peripheral	148:157	arg1	injury					165:170	peripheral nerve injury	148:170	peripheral nerve injury	148:170	The effective repair and functional recovery after peripheral nerve injury is still a hot topic in clinical research.					
30706639	6	68	theme	conduit	983:989	arg1	wall					966:969	the wall	962:969	the wall of hydrogel conduit	962:989	The results of diameter analysis indicated that the wall of hydrogel conduit was uniform.					
30706639	0	69	theme	nerve	83:87	arg1	injury					89:94	peripheral nerve injury	72:94	peripheral nerve injury	72:94	PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury.					
30706639	3	70	theme	2 mm	593:596	arg1	diameter					581:588	an inner diameter	572:588	an inner diameter of 2 mm and an outer diameter of 6 mm	572:626	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	3	71	theme	graphene	465:472	arg1	oxide					474:478	graphene oxide	465:478	graphene oxide	465:478	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	2	72	theme	defects	361:367	arg1	repair					333:338	the repair	329:338	the repair of rat sciatic nerve defects	329:367	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	3	73	theme	composite	374:382	arg1	conduit					393:399	The composite hydrogel conduit	370:399	The composite hydrogel conduit	370:399	The composite hydrogel conduit was fabricated by blending different volumes of polyacrylamide, graphene oxide, gelatin, and sodium alginate (PAM/GO/Gel/SA，PGGS) and injecting into a self-made mold with an inner diameter of 2 mm and an outer diameter of 6 mm.					
30706639	6	74	theme	hydrogel	974:981	arg1	conduit					983:989	hydrogel conduit	974:989	hydrogel conduit	974:989	The results of diameter analysis indicated that the wall of hydrogel conduit was uniform.					
30706639	0	75	theme	peripheral	72:81	arg1	injury					89:94	peripheral nerve injury	72:94	peripheral nerve injury	72:94	PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury.					
30706639	0	76	theme	hydrogel	24:31	arg1	conduit					33:39	PAM/GO/gel/SA composite hydrogel conduit	0:39	PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury.	0:95	PAM/GO/gel/SA composite hydrogel conduit with bioactivity for repairing peripheral nerve injury.					
30706639	2	77	theme	nerve	355:359	arg1	defects					361:367	rat sciatic nerve defects	343:367	rat sciatic nerve defects	343:367	The aim of this study was to construct a bioactive composite hydrogel conduit as a three-dimensional scaffold for the repair of rat sciatic nerve defects.					
30706639	7	78	contain	had	1069:1071	arg2	properties					1090:1099	suitable elastic properties	1073:1099	suitable elastic properties	1073:1099	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
30706639	7	78	contain	had	1069:1071	arg2	properties					1121:1130	good mechanical properties	1105:1130	good mechanical properties	1105:1130	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
30706639	7	78	contain	had	1069:1071	arg1	conduit					1061:1067	the hydrogel conduit	1048:1067	the hydrogel conduit	1048:1067	The tensile test of the conduit showed that the hydrogel conduit had suitable elastic properties and good mechanical properties.					
31590880	0	0	theme	whey	78:81	arg1	isolate					91:97	whey protein isolate	78:97	whey protein isolate	78:97	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	1	1	theme	gels	316:319	arg1	rheology					285:292	rheology	285:292	rheology	285:292	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	1	1	theme	gels	316:319	arg1	thermodynamics					269:282	thermodynamics	269:282	thermodynamics	269:282	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	1	1	theme	gels	316:319	arg1	texture					260:266	texture	260:266	texture	260:266	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	1	1	theme	gels	316:319	arg1	microstructure					298:311	microstructure	298:311	microstructure	298:311	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	2	2	dep	pH.	426:428	arg1	employed					481:488	employed	481:488	was accordingly employed to encapsulate vitamin D3	465:514	Results showed that pH at 7.0 induced a more compact and stable gel structure than other pH. The composite gel formed at pH 7.0 was accordingly employed to encapsulate vitamin D3.					
31590880	2	3	theme	vitamin	505:511	arg1	D3					513:514	vitamin D3	505:514	vitamin D3	505:514	Results showed that pH at 7.0 induced a more compact and stable gel structure than other pH. The composite gel formed at pH 7.0 was accordingly employed to encapsulate vitamin D3.					
31590880	3	4	theme	storage	644:650	arg1	stability					652:660	the storage stability	640:660	the storage stability of vitamin D3	640:674	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	0	5	theme	isolate	91:97	arg1	gels					70:73	composite gels	60:73	composite gels of whey protein isolate and lotus root amylopectin	60:124	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	1	6	dep	texture	260:266	arg1	The					256:258	The	256:258	The	256:258	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	6	7	dep	stability	1087:1095	arg1	the					1083:1085	the	1083:1085	the	1083:1085	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	0	8	theme	protein	83:89	arg1	isolate					91:97	whey protein isolate	78:97	whey protein isolate	78:97	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	5	9	theme	composite	954:962	arg1	gel					964:966	the composite gel	950:966	the composite gel	950:966	Animal experiments exhibited that the bioavailability was significantly increased after vitamin D3 was encapsulated by the composite gel.					
31590880	0	10	theme	lotus	103:107	arg1	amylopectin					114:124	lotus root amylopectin	103:124	lotus root amylopectin	103:124	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	3	11	theme	vitamin	688:694	arg1	D3					696:697	vitamin D3	688:697	vitamin D3 from photochemical degradation	688:728	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	4	12	theme	simulated	803:811	arg1	fluid					824:828	simulated intestinal fluid	803:828	simulated intestinal fluid	803:828	Moreover, this encapsulation could control the release of vitamin D3 in simulated intestinal fluid.					
31590880	3	13	theme	D3	673:674	arg1	stability					652:660	the storage stability	640:660	the storage stability of vitamin D3	640:674	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	2	14	theme	composite	434:442	arg1	gel					444:446	The composite gel	430:446	The composite gel formed at pH 7.0	430:463	Results showed that pH at 7.0 induced a more compact and stable gel structure than other pH. The composite gel formed at pH 7.0 was accordingly employed to encapsulate vitamin D3.					
31590880	5	15	theme	Animal	831:836	arg1	experiments					838:848	Animal experiments	831:848	Animal experiments	831:848	Animal experiments exhibited that the bioavailability was significantly increased after vitamin D3 was encapsulated by the composite gel.					
31590880	6	16	theme	good	1051:1054	arg1	gel					1042:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	6	16	theme	good	1051:1054	arg1	system					1065:1070	a good delivery system	1049:1070	a good delivery system to improve the stability and bioavailability of vitamin D3	1049:1129	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	3	17	theme	photochemical	704:716	arg1	degradation					718:728	photochemical degradation	704:728	photochemical degradation	704:728	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	3	18	from	degradation	718:728	arg1	D3					696:697	vitamin D3	688:697	vitamin D3 from photochemical degradation	688:728	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	1	19	theme	present	170:176	arg1	work					178:181	the present work	166:181	the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated	166:334	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	2	20	theme	gel	401:403	arg1	structure					405:413	a more compact and stable gel structure	375:413	a more compact and stable gel structure	375:413	Results showed that pH at 7.0 induced a more compact and stable gel structure than other pH. The composite gel formed at pH 7.0 was accordingly employed to encapsulate vitamin D3.					
31590880	0	21	theme	amylopectin	114:124	arg1	gels					70:73	composite gels	60:73	composite gels of whey protein isolate and lotus root amylopectin	60:124	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	6	22	theme	amylopectin	1030:1040	arg1	gel					1042:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	6	22	theme	amylopectin	1030:1040	arg1	system					1065:1070	a good delivery system	1049:1070	a good delivery system to improve the stability and bioavailability of vitamin D3	1049:1129	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	2	23	theme	stable	394:399	arg1	structure					405:413	a more compact and stable gel structure	375:413	a more compact and stable gel structure	375:413	Results showed that pH at 7.0 induced a more compact and stable gel structure than other pH. The composite gel formed at pH 7.0 was accordingly employed to encapsulate vitamin D3.					
31590880	0	24	theme	root	109:112	arg1	amylopectin					114:124	lotus root amylopectin	103:124	lotus root amylopectin	103:124	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	3	25	theme	lotus	603:607	arg1	amylopectin					614:624	lotus root amylopectin	603:624	lotus root amylopectin	603:624	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	6	26	theme	D3	1128:1129	arg1	bioavailability					1101:1115	bioavailability	1101:1115	bioavailability	1101:1115	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	6	26	theme	D3	1128:1129	arg1	stability					1087:1095	stability	1087:1095	stability	1087:1095	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	2	27	theme	compact	382:388	arg1	structure					405:413	a more compact and stable gel structure	375:413	a more compact and stable gel structure	375:413	Results showed that pH at 7.0 induced a more compact and stable gel structure than other pH. The composite gel formed at pH 7.0 was accordingly employed to encapsulate vitamin D3.					
31590880	0	28	theme	D3	41:42	arg1	bioavailability					14:28	bioavailability	14:28	bioavailability	14:28	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	0	28	theme	D3	41:42	arg1	Stability					0:8	Stability	0:8	Stability	0:8	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	3	29	theme	root	609:612	arg1	amylopectin					614:624	lotus root amylopectin	603:624	lotus root amylopectin	603:624	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	1	30	theme	whey	189:192	arg1	protein					194:200	whey protein isolate and lotus root amylopectin	189:235	protein	194:200	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	0	31	theme	vitamin	33:39	arg1	D3					41:42	vitamin D3	33:42	vitamin D3	33:42	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	3	32	theme	protein	583:589	arg1	encapsulation					544:556	the encapsulation	540:556	the encapsulation of composite gel of whey protein isolate and lotus root amylopectin	540:624	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	3	33	theme	composite	561:569	arg1	gel					571:573	composite gel	561:573	composite gel	561:573	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	3	34	dep	protein	583:589	arg1	isolate					591:597	isolate	591:597	isolate	591:597	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	4	35	theme	D3	797:798	arg1	release					778:784	the release	774:784	the release of vitamin D3 in simulated intestinal fluid	774:828	Moreover, this encapsulation could control the release of vitamin D3 in simulated intestinal fluid.					
31590880	4	36	theme	intestinal	813:822	arg1	fluid					824:828	simulated intestinal fluid	803:828	simulated intestinal fluid	803:828	Moreover, this encapsulation could control the release of vitamin D3 in simulated intestinal fluid.					
31590880	6	37	theme	vitamin	1120:1126	arg1	D3					1128:1129	vitamin D3	1120:1129	vitamin D3	1120:1129	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	4	38	theme	vitamin	789:795	arg1	D3					797:798	vitamin D3	789:798	vitamin D3	789:798	Moreover, this encapsulation could control the release of vitamin D3 in simulated intestinal fluid.					
31590880	6	39	theme	whey	998:1001	arg1	gel					1042:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	6	39	theme	whey	998:1001	arg1	system					1065:1070	a good delivery system	1049:1070	a good delivery system to improve the stability and bioavailability of vitamin D3	1049:1129	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	1	40	dep	pH.	252:254	arg1	evaluated					326:334	evaluated	326:334	were evaluated	321:334	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	3	41	theme	whey	578:581	arg1	protein					583:589	whey protein isolate and lotus root amylopectin	578:624	protein	583:589	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	2	42	from	7.0	363:365	arg1	pH					357:358	pH	357:358	pH at 7.0	357:365	Results showed that pH at 7.0 induced a more compact and stable gel structure than other pH. The composite gel formed at pH 7.0 was accordingly employed to encapsulate vitamin D3.					
31590880	6	43	theme	isolate-lotus	1011:1023	arg1	gel					1042:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	6	43	theme	isolate-lotus	1011:1023	arg1	system					1065:1070	a good delivery system	1049:1070	a good delivery system to improve the stability and bioavailability of vitamin D3	1049:1129	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	4	44	from	release	778:784	arg1	fluid					824:828	simulated intestinal fluid	803:828	simulated intestinal fluid	803:828	Moreover, this encapsulation could control the release of vitamin D3 in simulated intestinal fluid.					
31590880	3	45	theme	amylopectin	614:624	arg1	encapsulation					544:556	the encapsulation	540:556	the encapsulation of composite gel of whey protein isolate and lotus root amylopectin	540:624	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	1	46	dep	protein	194:200	arg1	isolate					202:208	isolate	202:208	isolate	202:208	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	5	47	theme	vitamin	919:925	arg1	D3					927:928	vitamin D3	919:928	vitamin D3	919:928	Animal experiments exhibited that the bioavailability was significantly increased after vitamin D3 was encapsulated by the composite gel.					
31590880	6	48	theme	protein	1003:1009	arg1	gel					1042:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	6	48	theme	protein	1003:1009	arg1	system					1065:1070	a good delivery system	1049:1070	a good delivery system to improve the stability and bioavailability of vitamin D3	1049:1129	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	6	49	theme	root	1025:1028	arg1	gel					1042:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	6	49	theme	root	1025:1028	arg1	system					1065:1070	a good delivery system	1049:1070	a good delivery system to improve the stability and bioavailability of vitamin D3	1049:1129	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	3	50	theme	gel	571:573	arg1	encapsulation					544:556	the encapsulation	540:556	the encapsulation of composite gel of whey protein isolate and lotus root amylopectin	540:624	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	1	51	theme	gel	129:131	arg1	system					142:147	A gel delivery system	127:147	A gel delivery system	127:147	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	6	52	theme	delivery	1056:1063	arg1	gel					1042:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	the whey protein isolate-lotus root amylopectin gel	994:1044	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	6	52	theme	delivery	1056:1063	arg1	system					1065:1070	a good delivery system	1049:1070	a good delivery system to improve the stability and bioavailability of vitamin D3	1049:1129	This work indicated that the whey protein isolate-lotus root amylopectin gel is a good delivery system to improve the stability and bioavailability of vitamin D3.					
31590880	1	53	theme	lotus	214:218	arg1	amylopectin					225:235	lotus root amylopectin	214:235	lotus root amylopectin	214:235	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	0	54	theme	composite	60:68	arg1	gels					70:73	composite gels	60:73	composite gels of whey protein isolate and lotus root amylopectin	60:124	Stability and bioavailability of vitamin D3 encapsulated in composite gels of whey protein isolate and lotus root amylopectin.					
31590880	3	55	theme	vitamin	665:671	arg1	D3					673:674	vitamin D3	665:674	vitamin D3	665:674	Results exhibited that the encapsulation of composite gel of whey protein isolate and lotus root amylopectin could enhance the storage stability of vitamin D3 and protect vitamin D3 from photochemical degradation.					
31590880	1	56	theme	delivery	133:140	arg1	system					142:147	A gel delivery system	127:147	A gel delivery system	127:147	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
31590880	1	57	theme	root	220:223	arg1	amylopectin					225:235	lotus root amylopectin	214:235	lotus root amylopectin	214:235	A gel delivery system was developed in the present work using whey protein isolate and lotus root amylopectin via regulating pH. The texture, thermodynamics, rheology and microstructure of gels were evaluated.					
30940497	7	0	theme	drug	1169:1172	arg1	accumulation					1174:1185	drug accumulation	1169:1185	drug accumulation in lungs and blood	1169:1204	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	13	1	theme	pharmacokinetic	2030:2044	arg1	study					2046:2050	The in-vivo pharmacokinetic study	2018:2050	The in-vivo pharmacokinetic study	2018:2050	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	6	2	theme	Selected	939:946	arg1	formulations					948:959	Selected formulations	939:959	Selected formulations	939:959	Selected formulations were also evaluated for swelling, biodegradation, moisture content, in-vitro aerodynamic performance, and cytotoxicity.					
30940497	12	3	theme	aerodynamic	1841:1851	arg1	properties					1853:1862	The aerodynamic properties	1837:1862	The aerodynamic properties	1837:1862	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	4	4	theme	Vertices	635:642	arg1	design					653:658	the "Extreme Vertices Mixture" design	622:658	the "Extreme Vertices Mixture" design	622:658	A design of experiment using the "Extreme Vertices Mixture" design was executed.					
30940497	5	5	used	used	689:692	arg2	design					678:683	The design	674:683	The design	674:683	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	10	6	theme	microparticulate	1689:1704	arg1	composition					1706:1716	the microparticulate composition	1685:1716	the microparticulate composition	1685:1716	The statistical design showed a significant influence of the microparticulate composition on the physicochemical properties.					
30940497	5	7	theme	efficiency	900:909	arg1	terms					822:826	terms	822:826	terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	822:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	10	8	from	influence	1672:1680	arg1	properties					1741:1750	the physicochemical properties	1721:1750	the physicochemical properties	1721:1750	The statistical design showed a significant influence of the microparticulate composition on the physicochemical properties.					
30940497	14	9	theme	sildenafil	2444:2453	arg1	citrate					2455:2461	sildenafil citrate	2444:2461	sildenafil citrate	2444:2461	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	1	10	theme	oral	210:213	arg1	treatment					215:223	the intravenous and oral treatment	190:223	the intravenous and oral treatment of pulmonary arterial hypertension (PAH)	190:264	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	6	11	theme	moisture	1011:1018	arg1	content					1020:1026	moisture content	1011:1026	moisture content	1011:1026	Selected formulations were also evaluated for swelling, biodegradation, moisture content, in-vitro aerodynamic performance, and cytotoxicity.					
30940497	7	12	theme	pharmacokinetic	1116:1130	arg1	study					1132:1136	pharmacokinetic study	1116:1136	pharmacokinetic study	1116:1136	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	15	13	theme	dried	2474:2478	arg1	alternative					2549:2559	a potential alternative	2537:2559	a potential alternative of oral sildenafil citrate for treatment of PAH	2537:2607	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	15	13	theme	dried	2474:2478	arg1	formulation					2504:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	5	14	theme	product	874:880	arg1	yield					882:886	product yield	874:886	product yield	874:886	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	8	15	contain	had	1396:1398	arg1	microparticles					1381:1394	formulated microparticles	1370:1394	formulated microparticles	1370:1394	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	8	15	contain	had	1396:1398	arg2	size					1426:1429	a mean geometric particle size	1400:1429	a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w	1400:1521	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	5	16	theme	interactions	752:763	arg1	influence					707:715	the influence	703:715	the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	703:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	13	17	theme	residence	2201:2209	arg1	time					2211:2214	mean residence time	2196:2214	mean residence time	2196:2214	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	1	18	theme	arterial	238:245	arg1	PAH					261:263	PAH	261:263	PAH	261:263	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	1	18	theme	arterial	238:245	arg1	hypertension					247:258	pulmonary arterial hypertension	228:258	pulmonary arterial hypertension (PAH)	228:264	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	5	19	from	formulations	806:817	arg1	terms					822:826	terms	822:826	terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	822:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	15	20	theme	citrate	2580:2586	arg1	alternative					2549:2559	a potential alternative	2537:2559	a potential alternative of oral sildenafil citrate for treatment of PAH	2537:2607	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	15	20	theme	citrate	2580:2586	arg1	formulation					2504:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	9	21	theme	drug	1537:1540	arg1	profiles					1550:1557	The in-vitro drug release profiles	1524:1557	The in-vitro drug release profiles	1524:1557	The in-vitro drug release profiles showed a sustained drug release of sildenafil citrate for over 24 h.					
30940497	3	22	contain	containing	445:454	arg2	alginate					509:516	sodium alginate	502:516	sodium alginate	502:516	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	3	22	contain	containing	445:454	arg2	cellulose					491:499	biodegradable sodium carboxymethyl cellulose	456:499	biodegradable sodium carboxymethyl cellulose	456:499	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	3	22	contain	containing	445:454	arg2	polymers					542:549	sodium hyaluronate polymers	523:549	sodium hyaluronate polymers	523:549	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	3	22	contain	containing	445:454	arg1	microparticles					430:443	Spray dried hydrogel microparticles	409:443	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations	409:576	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	14	23	theme	pulmonary	2422:2430	arg1	delivery					2432:2439	the pulmonary delivery	2418:2439	the pulmonary delivery of sildenafil citrate	2418:2461	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	5	24	theme	polymer	720:726	arg1	concentration					728:740	polymer concentration	720:740	polymer concentration	720:740	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	15	25	theme	hydrogel	2480:2487	arg1	alternative					2549:2559	a potential alternative	2537:2559	a potential alternative of oral sildenafil citrate for treatment of PAH	2537:2607	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	15	25	theme	hydrogel	2480:2487	arg1	formulation					2504:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	9	26	theme	release	1542:1548	arg1	profiles					1550:1557	The in-vitro drug release profiles	1524:1557	The in-vitro drug release profiles	1524:1557	The in-vitro drug release profiles showed a sustained drug release of sildenafil citrate for over 24 h.					
30940497	11	27	theme	aerodynamic	1813:1823	arg1	properties					1825:1834	their aerodynamic properties	1807:1834	their aerodynamic properties	1807:1834	Furthermore, selected formulations were evaluated for their aerodynamic properties.					
30940497	13	28	theme	lung/blood	2150:2159	arg1	Cmax					2161:2164	significantly higher lung/blood Cmax	2129:2164	significantly higher lung/blood Cmax	2129:2164	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	5	29	theme	drug	925:928	arg1	release					930:936	in-vitro drug release	916:936	in-vitro drug release	916:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	13	30	theme	mean	2196:2199	arg1	time					2211:2214	mean residence time	2196:2214	mean residence time	2196:2214	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	8	31	theme	geometric	1407:1415	arg1	size					1426:1429	a mean geometric particle size	1400:1429	a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w	1400:1521	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	14	32	theme	in-vivo	2398:2404	arg1	results					2406:2412	promising in-vitro and in-vivo results	2375:2412	promising in-vitro and in-vivo results	2375:2412	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	7	33	theme	hydrogel	1270:1277	arg1	microparticles					1279:1292	the spray dried inhalable hydrogel microparticles	1244:1292	the spray dried inhalable hydrogel microparticles	1244:1292	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	5	34	from	terms	822:826	arg1	properties					788:797	the physicochemical properties	768:797	the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	768:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	12	35	theme	median	1975:1980	arg1	diameter					1994:2001	average mass median aerodynamic diameter	1962:2001	average mass median aerodynamic diameter of 4.6-4.8 μm	1962:2015	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	9	36	theme	drug	1578:1581	arg1	release					1583:1589	a sustained drug release	1566:1589	a sustained drug release of sildenafil citrate	1566:1611	The in-vitro drug release profiles showed a sustained drug release of sildenafil citrate for over 24 h.					
30940497	13	37	contain	had	2125:2127	arg2	AUC					2167:2169	AUC	2167:2169	AUC	2167:2169	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	13	37	contain	had	2125:2127	arg2	time					2211:2214	mean residence time	2196:2214	mean residence time	2196:2214	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	13	37	contain	had	2125:2127	arg1	formulation					2113:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	13	37	contain	had	2125:2127	arg2	Cmax					2161:2164	significantly higher lung/blood Cmax	2129:2164	significantly higher lung/blood Cmax	2129:2164	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	13	37	contain	had	2125:2127	arg2	half-life					2181:2189	extended half-life	2172:2189	extended half-life	2172:2189	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	6	38	theme	aerodynamic	1038:1048	arg1	performance					1050:1060	in-vitro aerodynamic performance	1029:1060	in-vitro aerodynamic performance	1029:1060	Selected formulations were also evaluated for swelling, biodegradation, moisture content, in-vitro aerodynamic performance, and cytotoxicity.					
30940497	7	39	theme	inhalable	1260:1268	arg1	microparticles					1279:1292	the spray dried inhalable hydrogel microparticles	1244:1292	the spray dried inhalable hydrogel microparticles	1244:1292	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	14	40	theme	in-vitro	2385:2392	arg1	results					2406:2412	promising in-vitro and in-vivo results	2375:2412	promising in-vitro and in-vivo results	2375:2412	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	0	41	theme	arterial	101:108	arg1	hypertension					110:121	pulmonary arterial hypertension	91:121	pulmonary arterial hypertension	91:121	Design and evaluation of novel inhalable sildenafil citrate spray-dried microparticles for pulmonary arterial hypertension.					
30940497	3	42	theme	sodium	470:475	arg1	cellulose					491:499	biodegradable sodium carboxymethyl cellulose	456:499	biodegradable sodium carboxymethyl cellulose	456:499	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	15	43	theme	microparticles	2489:2502	arg1	alternative					2549:2559	a potential alternative	2537:2559	a potential alternative of oral sildenafil citrate for treatment of PAH	2537:2607	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	15	43	theme	microparticles	2489:2502	arg1	formulation					2504:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	9	44	theme	citrate	1605:1611	arg1	release					1583:1589	a sustained drug release	1566:1589	a sustained drug release of sildenafil citrate	1566:1611	The in-vitro drug release profiles showed a sustained drug release of sildenafil citrate for over 24 h.					
30940497	7	45	from	administration	1226:1239	arg1	comparison					1297:1306	comparison	1297:1306	comparison to orally administered Viagra®	1297:1337	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	14	46	theme	hydrogel	2344:2351	arg1	microparticles					2353:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	2	47	theme	sildenafil	389:398	arg1	citrate					400:406	sildenafil citrate	389:406	sildenafil citrate	389:406	The aim of this study was to design and evaluate hydrogel microparticles as a carrier for sustained pulmonary delivery of sildenafil citrate.					
30940497	5	48	theme	particle	846:853	arg1	distribution					860:871	particle size distribution	846:871	particle size distribution	846:871	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	12	49	theme	average	1962:1968	arg1	diameter					1994:2001	average mass median aerodynamic diameter	1962:2001	average mass median aerodynamic diameter of 4.6-4.8 μm	1962:2015	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	15	50	theme	PAH	2605:2607	arg1	treatment					2592:2600	treatment	2592:2600	treatment of PAH	2592:2607	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	8	51	theme	entrapment	1451:1460	arg1	%					1479:1479	entrapment efficiency of >80%	1451:1479	entrapment efficiency of >80%	1451:1479	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	14	52	theme	spray	2332:2336	arg1	microparticles					2353:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	3	53	theme	sodium	502:507	arg1	alginate					509:516	sodium alginate	502:516	sodium alginate	502:516	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	5	54	theme	distribution	860:871	arg1	terms					822:826	terms	822:826	terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	822:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	1	55	theme	Pulmonary	124:132	arg1	delivery					134:141	Pulmonary delivery	124:141	Pulmonary delivery of vasodilators	124:157	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	1	55	theme	Pulmonary	124:132	arg1	alternative					174:184	a promising alternative	162:184	a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH)	162:264	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	3	56	theme	hydrogel	421:428	arg1	microparticles					430:443	Spray dried hydrogel microparticles	409:443	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations	409:576	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	4	57	theme	experiment	605:614	arg1	design					595:600	A design	593:600	A design of experiment using the "Extreme Vertices Mixture" design	593:658	A design of experiment using the "Extreme Vertices Mixture" design was executed.					
30940497	8	58	theme	of	1473:1474	arg1	%					1479:1479	entrapment efficiency of >80%	1451:1479	entrapment efficiency of >80%	1451:1479	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	14	59	theme	formulated	2309:2318	arg1	microparticles					2353:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	10	60	theme	physicochemical	1725:1739	arg1	properties					1741:1750	the physicochemical properties	1721:1750	the physicochemical properties	1721:1750	The statistical design showed a significant influence of the microparticulate composition on the physicochemical properties.					
30940497	0	61	theme	novel	25:29	arg1	citrate					52:58	novel inhalable sildenafil citrate	25:58	novel inhalable sildenafil citrate	25:58	Design and evaluation of novel inhalable sildenafil citrate spray-dried microparticles for pulmonary arterial hypertension.					
30940497	3	62	theme	Spray	409:413	arg1	microparticles					430:443	Spray dried hydrogel microparticles	409:443	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations	409:576	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	3	63	theme	sodium	523:528	arg1	polymers					542:549	sodium hyaluronate polymers	523:549	sodium hyaluronate polymers	523:549	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	5	64	theme	size	840:843	arg1	terms					822:826	terms	822:826	terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	822:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	13	65	theme	dose	2284:2287	arg1	comparison					2219:2228	comparison	2219:2228	comparison to orally administered sildenafil citrate of the same dose	2219:2287	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	1	66	theme	hypertension	247:258	arg1	treatment					215:223	the intravenous and oral treatment	190:223	the intravenous and oral treatment of pulmonary arterial hypertension (PAH)	190:264	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	12	67	theme	recovery	1929:1936	arg1	percent					1938:1944	dose recovery percent	1924:1944	dose recovery percent of 68-8 5%	1924:1955	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	1	68	theme	promising	164:172	arg1	delivery					134:141	Pulmonary delivery	124:141	Pulmonary delivery of vasodilators	124:157	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	1	68	theme	promising	164:172	arg1	alternative					174:184	a promising alternative	162:184	a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH)	162:264	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	4	69	theme	Mixture	644:650	arg1	design					653:658	the "Extreme Vertices Mixture" design	622:658	the "Extreme Vertices Mixture" design	622:658	A design of experiment using the "Extreme Vertices Mixture" design was executed.					
30940497	13	70	theme	spray	2072:2076	arg1	formulation					2113:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	3	71	theme	variable	554:561	arg1	concentrations					563:576	variable concentrations	554:576	variable concentrations	554:576	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	1	72	theme	intravenous	194:204	arg1	treatment					215:223	the intravenous and oral treatment	190:223	the intravenous and oral treatment of pulmonary arterial hypertension (PAH)	190:264	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	0	73	theme	citrate	52:58	arg1	evaluation					11:20	evaluation	11:20	evaluation	11:20	Design and evaluation of novel inhalable sildenafil citrate spray-dried microparticles for pulmonary arterial hypertension.					
30940497	0	73	theme	citrate	52:58	arg1	Design					0:5	Design	0:5	Design	0:5	Design and evaluation of novel inhalable sildenafil citrate spray-dried microparticles for pulmonary arterial hypertension.					
30940497	7	74	theme	administered	1318:1329	arg1	Viagra®					1331:1337	orally administered Viagra®	1311:1337	orally administered Viagra®	1311:1337	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	5	75	theme	formulations	806:817	arg1	properties					788:797	the physicochemical properties	768:797	the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	768:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	13	76	theme	hydrogel	2084:2091	arg1	formulation					2113:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	2	77	theme	sustained	357:365	arg1	delivery					377:384	sustained pulmonary delivery	357:384	sustained pulmonary delivery of sildenafil citrate	357:406	The aim of this study was to design and evaluate hydrogel microparticles as a carrier for sustained pulmonary delivery of sildenafil citrate.					
30940497	12	78	theme	fine	1873:1876	arg1	fraction					1887:1894	fine particle fraction	1873:1894	fine particle fraction ranging between 24 and 30%	1873:1921	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	9	79	theme	sustained	1568:1576	arg1	release					1583:1589	a sustained drug release	1566:1589	a sustained drug release of sildenafil citrate	1566:1611	The in-vitro drug release profiles showed a sustained drug release of sildenafil citrate for over 24 h.					
30940497	4	80	theme	Extreme	627:633	arg1	design					653:658	the "Extreme Vertices Mixture" design	622:658	the "Extreme Vertices Mixture" design	622:658	A design of experiment using the "Extreme Vertices Mixture" design was executed.					
30940497	2	81	theme	study	283:287	arg1	aim					271:273	The aim	267:273	The aim of this study	267:287	The aim of this study was to design and evaluate hydrogel microparticles as a carrier for sustained pulmonary delivery of sildenafil citrate.					
30940497	2	81	theme	study	283:287	arg1	design					296:301	design	296:301	design	296:301	The aim of this study was to design and evaluate hydrogel microparticles as a carrier for sustained pulmonary delivery of sildenafil citrate.					
30940497	5	82	theme	entrapment	889:898	arg1	efficiency					900:909	entrapment efficiency	889:909	entrapment efficiency	889:909	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	0	83	theme	sildenafil	41:50	arg1	citrate					52:58	novel inhalable sildenafil citrate	25:58	novel inhalable sildenafil citrate	25:58	Design and evaluation of novel inhalable sildenafil citrate spray-dried microparticles for pulmonary arterial hypertension.					
30940497	14	84	theme	citrate	2455:2461	arg1	delivery					2432:2439	the pulmonary delivery	2418:2439	the pulmonary delivery of sildenafil citrate	2418:2461	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	2	85	theme	hydrogel	316:323	arg1	microparticles					325:338	hydrogel microparticles	316:338	hydrogel microparticles	316:338	The aim of this study was to design and evaluate hydrogel microparticles as a carrier for sustained pulmonary delivery of sildenafil citrate.					
30940497	2	85	theme	hydrogel	316:323	arg1	carrier					345:351	a carrier	343:351	a carrier for sustained pulmonary delivery of sildenafil citrate	343:406	The aim of this study was to design and evaluate hydrogel microparticles as a carrier for sustained pulmonary delivery of sildenafil citrate.					
30940497	10	86	theme	statistical	1632:1642	arg1	design					1644:1649	The statistical design	1628:1649	The statistical design	1628:1649	The statistical design showed a significant influence of the microparticulate composition on the physicochemical properties.					
30940497	5	87	theme	concentration	728:740	arg1	influence					707:715	the influence	703:715	the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	703:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	13	88	theme	in-vivo	2022:2028	arg1	study					2046:2050	The in-vivo pharmacokinetic study	2018:2050	The in-vivo pharmacokinetic study	2018:2050	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	7	89	theme	lung	1096:1099	arg1	deposition					1101:1110	a lung deposition	1094:1110	a lung deposition	1094:1110	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	5	90	from	properties	788:797	arg1	terms					822:826	terms	822:826	terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	822:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	5	91	theme	yield	882:886	arg1	terms					822:826	terms	822:826	terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	822:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	12	92	theme	4.6-4.8 μm	2006:2015	arg1	percent					1938:1944	dose recovery percent	1924:1944	dose recovery percent of 68-8 5%	1924:1955	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	12	92	theme	4.6-4.8 μm	2006:2015	arg1	diameter					1994:2001	average mass median aerodynamic diameter	1962:2001	average mass median aerodynamic diameter of 4.6-4.8 μm	1962:2015	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	12	92	theme	4.6-4.8 μm	2006:2015	arg1	fraction					1887:1894	fine particle fraction	1873:1894	fine particle fraction ranging between 24 and 30%	1873:1921	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	10	93	theme	significant	1660:1670	arg1	influence					1672:1680	a significant influence	1658:1680	a significant influence of the microparticulate composition on the physicochemical properties	1658:1750	The statistical design showed a significant influence of the microparticulate composition on the physicochemical properties.					
30940497	15	94	theme	spray	2468:2472	arg1	alternative					2549:2559	a potential alternative	2537:2559	a potential alternative of oral sildenafil citrate for treatment of PAH	2537:2607	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	15	94	theme	spray	2468:2472	arg1	formulation					2504:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	9	95	theme	in-vitro	1528:1535	arg1	profiles					1550:1557	The in-vitro drug release profiles	1524:1557	The in-vitro drug release profiles	1524:1557	The in-vitro drug release profiles showed a sustained drug release of sildenafil citrate for over 24 h.					
30940497	8	96	theme	w/w	1519:1521	arg1	%					1517:1517	47 and 66% w/w	1508:1521	47 and 66% w/w	1508:1521	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	13	97	theme	extended	2172:2179	arg1	half-life					2181:2189	extended half-life	2172:2189	extended half-life	2172:2189	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	1	98	theme	vasodilators	146:157	arg1	delivery					134:141	Pulmonary delivery	124:141	Pulmonary delivery of vasodilators	124:157	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	1	98	theme	vasodilators	146:157	arg1	alternative					174:184	a promising alternative	162:184	a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH)	162:264	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	8	99	theme	formulated	1370:1379	arg1	microparticles					1381:1394	formulated microparticles	1370:1394	formulated microparticles	1370:1394	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	7	100	theme	microparticles	1279:1292	arg1	administration					1226:1239	intratracheal administration	1212:1239	intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®	1212:1337	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	15	101	theme	sildenafil	2569:2578	arg1	citrate					2580:2586	oral sildenafil citrate	2564:2586	oral sildenafil citrate	2564:2586	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	5	102	theme	in-vitro	916:923	arg1	release					930:936	in-vitro drug release	916:936	in-vitro drug release	916:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	7	103	from	accumulation	1174:1185	arg1	lungs					1190:1194	lungs	1190:1194	lungs	1190:1194	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	7	103	from	accumulation	1174:1185	arg1	blood					1200:1204	blood	1200:1204	blood	1200:1204	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	13	104	theme	administered	2240:2251	arg1	citrate					2264:2270	orally administered sildenafil citrate	2233:2270	orally administered sildenafil citrate	2233:2270	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	7	105	theme	dried	1254:1258	arg1	microparticles					1279:1292	the spray dried inhalable hydrogel microparticles	1244:1292	the spray dried inhalable hydrogel microparticles	1244:1292	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	0	106	theme	pulmonary	91:99	arg1	hypertension					110:121	pulmonary arterial hypertension	91:121	pulmonary arterial hypertension	91:121	Design and evaluation of novel inhalable sildenafil citrate spray-dried microparticles for pulmonary arterial hypertension.					
30940497	15	107	theme	potential	2539:2547	arg1	alternative					2549:2559	a potential alternative	2537:2559	a potential alternative of oral sildenafil citrate for treatment of PAH	2537:2607	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	15	107	theme	potential	2539:2547	arg1	formulation					2504:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation	2464:2514	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	12	108	theme	aerodynamic	1982:1992	arg1	diameter					1994:2001	average mass median aerodynamic diameter	1962:2001	average mass median aerodynamic diameter of 4.6-4.8 μm	1962:2015	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	5	109	theme	release	930:936	arg1	terms					822:826	terms	822:826	terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	822:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	15	110	theme	oral	2564:2567	arg1	citrate					2580:2586	oral sildenafil citrate	2564:2586	oral sildenafil citrate	2564:2586	The spray dried hydrogel microparticles formulation can be considered as a potential alternative of oral sildenafil citrate for treatment of PAH.					
30940497	10	111	theme	composition	1706:1716	arg1	influence					1672:1680	a significant influence	1658:1680	a significant influence of the microparticulate composition on the physicochemical properties	1658:1750	The statistical design showed a significant influence of the microparticulate composition on the physicochemical properties.					
30940497	8	112	theme	mean	1402:1405	arg1	size					1426:1429	a mean geometric particle size	1400:1429	a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w	1400:1521	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	7	113	theme	spray	1248:1252	arg1	microparticles					1279:1292	the spray dried inhalable hydrogel microparticles	1244:1292	the spray dried inhalable hydrogel microparticles	1244:1292	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	6	114	theme	in-vitro	1029:1036	arg1	performance					1050:1060	in-vitro aerodynamic performance	1029:1060	in-vitro aerodynamic performance	1029:1060	Selected formulations were also evaluated for swelling, biodegradation, moisture content, in-vitro aerodynamic performance, and cytotoxicity.					
30940497	8	115	theme	particle	1417:1424	arg1	size					1426:1429	a mean geometric particle size	1400:1429	a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w	1400:1521	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	14	116	theme	promising	2375:2383	arg1	results					2406:2412	promising in-vitro and in-vivo results	2375:2412	promising in-vitro and in-vivo results	2375:2412	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	5	117	theme	physicochemical	772:786	arg1	properties					788:797	the physicochemical properties	768:797	the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	768:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	9	118	theme	sildenafil	1594:1603	arg1	citrate					1605:1611	sildenafil citrate	1594:1611	sildenafil citrate	1594:1611	The in-vitro drug release profiles showed a sustained drug release of sildenafil citrate for over 24 h.					
30940497	2	119	theme	citrate	400:406	arg1	delivery					377:384	sustained pulmonary delivery	357:384	sustained pulmonary delivery of sildenafil citrate	357:406	The aim of this study was to design and evaluate hydrogel microparticles as a carrier for sustained pulmonary delivery of sildenafil citrate.					
30940497	7	120	theme	intratracheal	1212:1224	arg1	administration					1226:1239	intratracheal administration	1212:1239	intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®	1212:1337	In addition, a lung deposition and pharmacokinetic study was conducted in rats to study drug accumulation in lungs and blood after intratracheal administration of the spray dried inhalable hydrogel microparticles in comparison to orally administered Viagra®.					
30940497	13	121	theme	higher	2143:2148	arg1	Cmax					2161:2164	significantly higher lung/blood Cmax	2129:2164	significantly higher lung/blood Cmax	2129:2164	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	12	122	theme	mass	1970:1973	arg1	diameter					1994:2001	average mass median aerodynamic diameter	1962:2001	average mass median aerodynamic diameter of 4.6-4.8 μm	1962:2015	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	14	123	theme	dried	2338:2342	arg1	microparticles					2353:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	3	124	theme	biodegradable	456:468	arg1	cellulose					491:499	biodegradable sodium carboxymethyl cellulose	456:499	biodegradable sodium carboxymethyl cellulose	456:499	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	5	125	theme	size	855:858	arg1	distribution					860:871	particle size distribution	846:871	particle size distribution	846:871	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	12	126	theme	68-8	1949:1952	arg1	%					1955:1955	68-8 5%	1949:1955	68-8 5%	1949:1955	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	11	127	theme	selected	1766:1773	arg1	formulations					1775:1786	selected formulations	1766:1786	selected formulations	1766:1786	Furthermore, selected formulations were evaluated for their aerodynamic properties.					
30940497	14	128	theme	drug-loaded	2320:2330	arg1	microparticles					2353:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	the formulated drug-loaded spray dried hydrogel microparticles	2305:2366	In conclusion, the formulated drug-loaded spray dried hydrogel microparticles showed promising in-vitro and in-vivo results for the pulmonary delivery of sildenafil citrate.					
30940497	0	129	theme	inhalable	31:39	arg1	citrate					52:58	novel inhalable sildenafil citrate	25:58	novel inhalable sildenafil citrate	25:58	Design and evaluation of novel inhalable sildenafil citrate spray-dried microparticles for pulmonary arterial hypertension.					
30940497	12	130	theme	%	1955:1955	arg1	percent					1938:1944	dose recovery percent	1924:1944	dose recovery percent of 68-8 5%	1924:1955	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	12	130	theme	%	1955:1955	arg1	diameter					1994:2001	average mass median aerodynamic diameter	1962:2001	average mass median aerodynamic diameter of 4.6-4.8 μm	1962:2015	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	12	130	theme	%	1955:1955	arg1	fraction					1887:1894	fine particle fraction	1873:1894	fine particle fraction ranging between 24 and 30%	1873:1921	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	1	131	theme	pulmonary	228:236	arg1	PAH					261:263	PAH	261:263	PAH	261:263	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	1	131	theme	pulmonary	228:236	arg1	hypertension					247:258	pulmonary arterial hypertension	228:258	pulmonary arterial hypertension (PAH)	228:264	Pulmonary delivery of vasodilators is a promising alternative for the intravenous and oral treatment of pulmonary arterial hypertension (PAH).					
30940497	8	132	theme	efficiency	1462:1471	arg1	%					1479:1479	entrapment efficiency of >80%	1451:1479	entrapment efficiency of >80%	1451:1479	The results demonstrated that formulated microparticles had a mean geometric particle size between 2 and 5 μm, entrapment efficiency of >80%, and yield ranging between 47 and 66% w/w.					
30940497	13	133	theme	microparticles	2093:2106	arg1	formulation					2113:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	5	134	theme	particle	831:838	arg1	size					840:843	particle size	831:843	particle size	831:843	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
30940497	13	135	theme	same	2279:2282	arg1	dose					2284:2287	the same dose	2275:2287	the same dose	2275:2287	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	12	136	theme	dose	1924:1927	arg1	percent					1938:1944	dose recovery percent	1924:1944	dose recovery percent of 68-8 5%	1924:1955	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	3	137	theme	dried	415:419	arg1	microparticles					430:443	Spray dried hydrogel microparticles	409:443	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations	409:576	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	13	138	theme	M6	2109:2110	arg1	formulation					2113:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	3	139	theme	hyaluronate	530:540	arg1	polymers					542:549	sodium hyaluronate polymers	523:549	sodium hyaluronate polymers	523:549	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	4	140	theme	"	651:651	arg1	design					653:658	the "Extreme Vertices Mixture" design	622:658	the "Extreme Vertices Mixture" design	622:658	A design of experiment using the "Extreme Vertices Mixture" design was executed.					
30940497	13	141	theme	inhaled	2064:2070	arg1	formulation					2113:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	13	142	theme	sildenafil	2253:2262	arg1	citrate					2264:2270	orally administered sildenafil citrate	2233:2270	orally administered sildenafil citrate	2233:2270	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	12	143	theme	particle	1878:1885	arg1	fraction					1887:1894	fine particle fraction	1873:1894	fine particle fraction ranging between 24 and 30%	1873:1921	The aerodynamic properties included fine particle fraction ranging between 24 and 30%, dose recovery percent of 68-8 5%, and average mass median aerodynamic diameter of 4.6-4.8 μm.					
30940497	3	144	theme	carboxymethyl	477:489	arg1	cellulose					491:499	biodegradable sodium carboxymethyl cellulose	456:499	biodegradable sodium carboxymethyl cellulose	456:499	Spray dried hydrogel microparticles containing biodegradable sodium carboxymethyl cellulose, sodium alginate, and sodium hyaluronate polymers at variable concentrations were prepared.					
30940497	13	145	theme	dried	2078:2082	arg1	formulation					2113:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	inhaled spray dried hydrogel microparticles (M6) formulation	2064:2123	The in-vivo pharmacokinetic study showed that inhaled spray dried hydrogel microparticles (M6) formulation had significantly higher lung/blood Cmax, AUC, extended half-life, and mean residence time in comparison to orally administered sildenafil citrate of the same dose.					
30940497	2	146	theme	pulmonary	367:375	arg1	delivery					377:384	sustained pulmonary delivery	357:384	sustained pulmonary delivery of sildenafil citrate	357:406	The aim of this study was to design and evaluate hydrogel microparticles as a carrier for sustained pulmonary delivery of sildenafil citrate.					
30940497	5	147	from	influence	707:715	arg1	properties					788:797	the physicochemical properties	768:797	the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release	768:936	The design was used to study the influence of polymer concentration and their interactions on the physicochemical properties of the formulations in terms of particle size, particle size distribution, product yield, entrapment efficiency, and in-vitro drug release.					
29663371	6	0	dep	collagens	803:811	arg1	fibromodulin					851:862	fibromodulin	851:862	fibromodulin	851:862	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	6	0	dep	collagens	803:811	arg1	biglycan					841:848	biglycan	841:848	biglycan	841:848	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	6	0	dep	collagens	803:811	arg1	collagens					803:811	III collagens	799:811	III collagens	799:811	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	6	0	dep	collagens	803:811	arg1	decorin					832:838	decorin	832:838	decorin	832:838	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	6	0	dep	collagens	803:811	arg1	versican					868:875	versican	868:875	versican	868:875	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	13	1	theme	specific	1991:1998	arg1	role					2000:2003	the specific role	1987:2003	the specific role of each molecule in the human UT	1987:2036	However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.					
29663371	1	2	theme	system	231:236	arg1	tube					159:162	The uterine tube	147:162	The uterine tube (UT)	147:167	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	1	2	theme	system	231:236	arg1	organ					197:201	an important and complex organ	172:201	an important and complex organ of the women's reproductive system	172:236	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	12	3	theme	successful	1824:1833	arg1	fertilization					1835:1847	successful fertilization	1824:1847	successful fertilization	1824:1847	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	7	4	theme	broad	1010:1014	arg1	synthesis					1016:1024	a broad synthesis	1008:1024	a broad synthesis of collagens (I and III) in the stroma of the UT	1008:1073	Our results showed a broad synthesis of collagens (I and III) in the stroma of the UT.					
29663371	13	5	theme	molecule	2013:2020	arg1	role					2000:2003	the specific role	1987:2003	the specific role of each molecule in the human UT	1987:2036	However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.					
29663371	8	6	located	observed	1241:1248	arg2	fibromodulin					1223:1234	fibromodulin	1223:1234	fibromodulin	1223:1234	The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria.					
29663371	8	6	located	observed	1241:1248	arg2	decorin					1211:1217	decorin	1211:1217	decorin	1211:1217	The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria.					
29663371	8	6	located	observed	1241:1248	arg1	propria					1264:1270	the lamina propria	1253:1270	the lamina propria	1253:1270	The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria.					
29663371	11	7	theme	UT	1608:1609	arg1	nerves					1622:1627	the UT peripheral nerves	1604:1627	the UT peripheral nerves	1604:1627	Furthermore, there was an immunoreaction for collagens, decorin, versican and fibromodulin in the UT peripheral nerves.					
29663371	12	8	theme	biomechanical	1764:1776	arg1	functions					1778:1786	specific structural and/or biomechanical functions	1737:1786	specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis	1737:1871	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	5	9	from	growth	645:650	arg1	tissues					689:695	all tissues	685:695	all tissues	685:695	ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues.					
29663371	0	10	from	tubes	64:68	arg1	Distribution					0:11	Distribution	0:11	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle	0:95	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	9	11	theme	layer	1335:1339	arg1	stroma					1312:1317	the stroma	1308:1317	the stroma of the muscular layer	1308:1339	Versican and decorin were found in the stroma of the muscular layer, whereas all studied proteoglycans were identified in the serosa.					
29663371	8	12	theme	versican	1150:1157	arg1	distribution					1134:1145	a specific distribution	1123:1145	a specific distribution of versican and fibromodulin in the epithelial surface	1123:1200	The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria.					
29663371	6	13	theme	follicular	901:910	arg1	phases					923:928	the follicular and luteal phases	897:928	the follicular and luteal phases	897:928	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	8	14	theme	fibromodulin	1163:1174	arg1	distribution					1134:1145	a specific distribution	1123:1145	a specific distribution of versican and fibromodulin in the epithelial surface	1123:1200	The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria.					
29663371	9	15	theme	studied	1354:1360	arg1	proteoglycans					1362:1374	all studied proteoglycans	1350:1374	all studied proteoglycans	1350:1374	Versican and decorin were found in the stroma of the muscular layer, whereas all studied proteoglycans were identified in the serosa.					
29663371	3	16	dep	composition	326:336	arg1	the					322:324	the	322:324	the	322:324	However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood.					
29663371	2	17	dep	anatomy	255:261	arg1	the					251:253	the	251:253	the	251:253	In general, the anatomy and basic histology of this organ are well-known.					
29663371	6	18	theme	human	881:885	arg1	UT					887:888	human UT	881:888	human UT	881:888	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	0	19	from	Distribution	0:11	arg1	tubes					64:68	human uterine tubes	50:68	human uterine tubes	50:68	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	7	20	theme	UT	1072:1073	arg1	stroma					1058:1063	the stroma	1054:1063	the stroma of the UT	1054:1073	Our results showed a broad synthesis of collagens (I and III) in the stroma of the UT.					
29663371	12	21	theme	specific	1737:1744	arg1	functions					1778:1786	specific structural and/or biomechanical functions	1737:1786	specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis	1737:1871	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	8	22	from	distribution	1134:1145	arg1	surface					1194:1200	the epithelial surface	1179:1200	the epithelial surface	1179:1200	The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria.					
29663371	7	23	dep	collagens	1029:1037	arg1	I					1040:1040	I	1040:1040	I	1040:1040	Our results showed a broad synthesis of collagens (I and III) in the stroma of the UT.					
29663371	7	23	dep	collagens	1029:1037	arg1	collagens					1029:1037	collagens	1029:1037	collagens (I and III)	1029:1049	Our results showed a broad synthesis of collagens (I and III) in the stroma of the UT.					
29663371	7	23	dep	collagens	1029:1037	arg1	III					1046:1048	III	1046:1048	III	1046:1048	Our results showed a broad synthesis of collagens (I and III) in the stroma of the UT.					
29663371	10	24	theme	serosa	1482:1487	arg1	vessels					1467:1473	the blood vessels	1457:1473	the blood vessels of the serosa and muscular layers	1457:1507	Curiously, biglycan was restricted to the wall of the blood vessels of the serosa and muscular layers.					
29663371	13	25	theme	molecular	1891:1899	arg1	studies					1901:1907	further molecular studies	1883:1907	further molecular studies under physiological and pathological conditions	1883:1955	However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.					
29663371	3	26	theme	matrix	372:377	arg1	function					342:349	function	342:349	function	342:349	However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood.					
29663371	3	26	theme	matrix	372:377	arg1	composition					326:336	composition	326:336	composition	326:336	However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood.					
29663371	6	27	theme	immunohistochemical	957:975	arg1	techniques					977:986	histochemical and immunohistochemical techniques	939:986	histochemical and immunohistochemical techniques	939:986	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	0	28	from	molecules	37:45	arg1	tubes					64:68	human uterine tubes	50:68	human uterine tubes	50:68	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	6	29	theme	histochemical	939:951	arg1	techniques					977:986	histochemical and immunohistochemical techniques	939:986	histochemical and immunohistochemical techniques	939:986	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	0	30	theme	extracellular	16:28	arg1	molecules					37:45	extracellular matrix molecules	16:45	extracellular matrix molecules in human uterine tubes	16:68	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	5	31	dep	play	579:582	arg1	only					588:591	only	588:591	only	588:591	ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues.					
29663371	13	32	from	role	2000:2003	arg1	UT					2035:2036	the human UT	2025:2036	the human UT	2025:2036	However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.					
29663371	10	33	theme	muscular	1493:1500	arg1	layers					1502:1507	muscular layers	1493:1507	muscular layers	1493:1507	Curiously, biglycan was restricted to the wall of the blood vessels of the serosa and muscular layers.					
29663371	9	34	dep	found	1299:1303	arg1	whereas					1342:1348	whereas	1342:1348	whereas	1342:1348	Versican and decorin were found in the stroma of the muscular layer, whereas all studied proteoglycans were identified in the serosa.					
29663371	3	35	theme	UT	392:393	arg1	ECM					380:382	ECM	380:382	ECM	380:382	However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood.					
29663371	3	35	theme	UT	392:393	arg1	matrix					372:377	the extracellular matrix	354:377	the extracellular matrix (ECM) of the UT	354:393	However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood.					
29663371	0	36	theme	molecules	37:45	arg1	Distribution					0:11	Distribution	0:11	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle	0:95	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	12	37	theme	differential	1634:1645	arg1	distribution					1647:1658	The differential distribution	1630:1658	The differential distribution of these ECM molecules in the different layers of the UT	1630:1715	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	12	37	theme	differential	1634:1645	arg1	related					1726:1732	related	1726:1732	related	1726:1732	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	6	38	theme	luteal	916:921	arg1	phases					923:928	the follicular and luteal phases	897:928	the follicular and luteal phases	897:928	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	8	39	theme	lamina	1257:1262	arg1	propria					1264:1270	the lamina propria	1253:1270	the lamina propria	1253:1270	The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria.					
29663371	0	40	theme	human	50:54	arg1	tubes					64:68	human uterine tubes	50:68	human uterine tubes	50:68	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	12	41	from	distribution	1647:1658	arg1	layers					1700:1705	the different layers	1686:1705	the different layers of the UT	1686:1715	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	2	42	theme	organ	291:295	arg1	anatomy					255:261	anatomy	255:261	anatomy	255:261	In general, the anatomy and basic histology of this organ are well-known.					
29663371	2	42	theme	organ	291:295	arg1	histology					273:281	basic histology	267:281	basic histology	267:281	In general, the anatomy and basic histology of this organ are well-known.					
29663371	12	43	theme	molecules	1673:1681	arg1	distribution					1647:1658	The differential distribution	1630:1658	The differential distribution of these ECM molecules in the different layers of the UT	1630:1715	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	12	43	theme	molecules	1673:1681	arg1	related					1726:1732	related	1726:1732	related	1726:1732	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	0	44	dep	analysis	137:144	arg1	Distribution					0:11	Distribution	0:11	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle	0:95	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	10	45	dep	serosa	1482:1487	arg1	the					1478:1480	the	1478:1480	the	1478:1480	Curiously, biglycan was restricted to the wall of the blood vessels of the serosa and muscular layers.					
29663371	13	46	theme	human	2029:2033	arg1	UT					2035:2036	the human UT	2025:2036	the human UT	2025:2036	However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.					
29663371	12	47	theme	early	1853:1857	arg1	embryogenesis					1859:1871	early embryogenesis	1853:1871	early embryogenesis	1853:1871	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	1	48	theme	reproductive	218:229	arg1	system					231:236	the women's reproductive system	206:236	the women's reproductive system	206:236	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	6	49	theme	collagens	803:811	arg1	deposition					757:766	deposition	757:766	deposition	757:766	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	6	49	theme	collagens	803:811	arg1	distribution					772:783	distribution	772:783	distribution	772:783	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	5	50	theme	structural	595:604	arg1	role					606:609	a structural role	593:609	a structural role	593:609	ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues.					
29663371	9	51	located	found	1299:1303	arg1	stroma					1312:1317	the stroma	1308:1317	the stroma of the muscular layer	1308:1339	Versican and decorin were found in the stroma of the muscular layer, whereas all studied proteoglycans were identified in the serosa.					
29663371	9	51	located	found	1299:1303	arg2	Versican					1273:1280	Versican	1273:1280	Versican	1273:1280	Versican and decorin were found in the stroma of the muscular layer, whereas all studied proteoglycans were identified in the serosa.					
29663371	9	51	located	found	1299:1303	arg2	decorin					1286:1292	decorin	1286:1292	decorin	1286:1292	Versican and decorin were found in the stroma of the muscular layer, whereas all studied proteoglycans were identified in the serosa.					
29663371	2	52	from	well-known	301:310	arg1	general					242:248	general	242:248	general	242:248	In general, the anatomy and basic histology of this organ are well-known.					
29663371	4	53	theme	basement	522:529	arg1	membrane					531:538	the basement membrane	518:538	the basement membrane	518:538	The ECM is a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces.					
29663371	5	54	theme	ECM	565:567	arg1	molecules					569:577	ECM molecules	565:577	ECM molecules	565:577	ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues.					
29663371	6	55	theme	type	788:791	arg1	deposition					757:766	deposition	757:766	deposition	757:766	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	6	55	theme	type	788:791	arg1	distribution					772:783	distribution	772:783	distribution	772:783	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	6	56	from	deposition	757:766	arg1	UT					887:888	human UT	881:888	human UT	881:888	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	6	57	from	distribution	772:783	arg1	UT					887:888	human UT	881:888	human UT	881:888	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	0	58	theme	immunohistochemical	117:135	arg1	analysis					137:144	a histological and immunohistochemical analysis	98:144	a histological and immunohistochemical analysis	98:144	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	2	59	theme	basic	267:271	arg1	histology					273:281	basic histology	267:281	basic histology	267:281	In general, the anatomy and basic histology of this organ are well-known.					
29663371	1	60	theme	important	175:183	arg1	tube					159:162	The uterine tube	147:162	The uterine tube (UT)	147:167	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	1	60	theme	important	175:183	arg1	organ					197:201	an important and complex organ	172:201	an important and complex organ of the women's reproductive system	172:236	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	12	61	theme	oviductal	1803:1811	arg1	transport					1813:1821	the oviductal transport	1799:1821	the oviductal transport	1799:1821	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	11	62	from	fibromodulin	1588:1599	arg1	nerves					1622:1627	the UT peripheral nerves	1604:1627	the UT peripheral nerves	1604:1627	Furthermore, there was an immunoreaction for collagens, decorin, versican and fibromodulin in the UT peripheral nerves.					
29663371	10	63	theme	blood	1461:1465	arg1	vessels					1467:1473	the blood vessels	1457:1473	the blood vessels of the serosa and muscular layers	1457:1507	Curiously, biglycan was restricted to the wall of the blood vessels of the serosa and muscular layers.					
29663371	11	64	theme	peripheral	1611:1620	arg1	nerves					1622:1627	the UT peripheral nerves	1604:1627	the UT peripheral nerves	1604:1627	Furthermore, there was an immunoreaction for collagens, decorin, versican and fibromodulin in the UT peripheral nerves.					
29663371	5	65	theme	cell	640:643	arg1	growth					645:650	cell growth	640:650	cell growth	640:650	ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues.					
29663371	5	66	from	differentiation	666:680	arg1	tissues					689:695	all tissues	685:695	all tissues	685:695	ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues.					
29663371	1	67	theme	complex	189:195	arg1	tube					159:162	The uterine tube	147:162	The uterine tube (UT)	147:167	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	1	67	theme	complex	189:195	arg1	organ					197:201	an important and complex organ	172:201	an important and complex organ of the women's reproductive system	172:236	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	5	68	from	survival	653:660	arg1	tissues					689:695	all tissues	685:695	all tissues	685:695	ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues.					
29663371	9	69	theme	muscular	1326:1333	arg1	layer					1335:1339	the muscular layer	1322:1339	the muscular layer	1322:1339	Versican and decorin were found in the stroma of the muscular layer, whereas all studied proteoglycans were identified in the serosa.					
29663371	4	70	theme	complex	436:442	arg1	material					459:466	a complex supramolecular material	434:466	a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces	434:562	The ECM is a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces.					
29663371	4	70	theme	complex	436:442	arg1	ECM					427:429	The ECM	423:429	The ECM	423:429	The ECM is a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces.					
29663371	11	71	from	decorin	1566:1572	arg1	nerves					1622:1627	the UT peripheral nerves	1604:1627	the UT peripheral nerves	1604:1627	Furthermore, there was an immunoreaction for collagens, decorin, versican and fibromodulin in the UT peripheral nerves.					
29663371	8	72	theme	specific	1125:1132	arg1	distribution					1134:1145	a specific distribution	1123:1145	a specific distribution of versican and fibromodulin in the epithelial surface	1123:1200	The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria.					
29663371	2	73	from	general	242:248	arg1	well-known					301:310	well-known	301:310	well-known	301:310	In general, the anatomy and basic histology of this organ are well-known.					
29663371	11	74	from	versican	1575:1582	arg1	nerves					1622:1627	the UT peripheral nerves	1604:1627	the UT peripheral nerves	1604:1627	Furthermore, there was an immunoreaction for collagens, decorin, versican and fibromodulin in the UT peripheral nerves.					
29663371	0	75	theme	menstrual	81:89	arg1	cycle					91:95	the menstrual cycle	77:95	the menstrual cycle	77:95	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	13	76	theme	physiological	1915:1927	arg1	conditions					1946:1955	physiological and pathological conditions	1915:1955	physiological and pathological conditions	1915:1955	However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.					
29663371	4	77	theme	supramolecular	444:457	arg1	material					459:466	a complex supramolecular material	434:466	a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces	434:562	The ECM is a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces.					
29663371	4	77	theme	supramolecular	444:457	arg1	ECM					427:429	The ECM	423:429	The ECM	423:429	The ECM is a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces.					
29663371	13	78	theme	pathological	1933:1944	arg1	conditions					1946:1955	physiological and pathological conditions	1915:1955	physiological and pathological conditions	1915:1955	However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.					
29663371	12	79	theme	structural	1746:1755	arg1	functions					1778:1786	specific structural and/or biomechanical functions	1737:1786	specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis	1737:1871	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	8	80	theme	epithelial	1183:1192	arg1	surface					1194:1200	the epithelial surface	1179:1200	the epithelial surface	1179:1200	The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria.					
29663371	0	81	theme	histological	100:111	arg1	analysis					137:144	a histological and immunohistochemical analysis	98:144	a histological and immunohistochemical analysis	98:144	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	13	82	theme	further	1883:1889	arg1	studies					1901:1907	further molecular studies	1883:1907	further molecular studies under physiological and pathological conditions	1883:1955	However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.					
29663371	12	83	theme	UT	1714:1715	arg1	layers					1700:1705	the different layers	1686:1705	the different layers of the UT	1686:1715	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	10	84	theme	vessels	1467:1473	arg1	wall					1449:1452	the wall	1445:1452	the wall of the blood vessels of the serosa and muscular layers	1445:1507	Curiously, biglycan was restricted to the wall of the blood vessels of the serosa and muscular layers.					
29663371	0	85	theme	matrix	30:35	arg1	molecules					37:45	extracellular matrix molecules	16:45	extracellular matrix molecules in human uterine tubes	16:68	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	12	86	theme	different	1690:1698	arg1	layers					1700:1705	the different layers	1686:1705	the different layers of the UT	1686:1715	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	10	87	theme	layers	1502:1507	arg1	vessels					1467:1473	the blood vessels	1457:1473	the blood vessels of the serosa and muscular layers	1457:1507	Curiously, biglycan was restricted to the wall of the blood vessels of the serosa and muscular layers.					
29663371	7	88	theme	collagens	1029:1037	arg1	synthesis					1016:1024	a broad synthesis	1008:1024	a broad synthesis of collagens (I and III) in the stroma of the UT	1008:1073	Our results showed a broad synthesis of collagens (I and III) in the stroma of the UT.					
29663371	6	89	dep	deposition	757:766	arg1	the					753:755	the	753:755	the	753:755	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
29663371	0	90	theme	uterine	56:62	arg1	tubes					64:68	human uterine tubes	50:68	human uterine tubes	50:68	Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis.					
29663371	12	91	theme	ECM	1669:1671	arg1	molecules					1673:1681	these ECM molecules	1663:1681	these ECM molecules	1663:1681	The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis.					
29663371	3	92	theme	extracellular	358:370	arg1	ECM					380:382	ECM	380:382	ECM	380:382	However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood.					
29663371	3	92	theme	extracellular	358:370	arg1	matrix					372:377	the extracellular matrix	354:377	the extracellular matrix (ECM) of the UT	354:393	However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood.					
29663371	7	93	from	synthesis	1016:1024	arg1	stroma					1058:1063	the stroma	1054:1063	the stroma of the UT	1054:1073	Our results showed a broad synthesis of collagens (I and III) in the stroma of the UT.					
29663371	1	94	theme	uterine	151:157	arg1	UT					165:166	UT	165:166	UT	165:166	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	1	94	theme	uterine	151:157	arg1	tube					159:162	The uterine tube	147:162	The uterine tube (UT)	147:167	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	1	94	theme	uterine	151:157	arg1	organ					197:201	an important and complex organ	172:201	an important and complex organ of the women's reproductive system	172:236	The uterine tube (UT) is an important and complex organ of the women's reproductive system.					
29663371	4	95	theme	interstitial	544:555	arg1	spaces					557:562	interstitial spaces	544:562	interstitial spaces	544:562	The ECM is a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces.					
29663371	6	96	theme	study	731:735	arg1	aim					719:721	the aim	715:721	the aim of this study	715:735	In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques.					
30348953	7	0	theme	digital	983:989	arg1	densitometry					991:1002	digital densitometry	983:1002	digital densitometry	983:1002	FCD content at different follow-up time points was assessed using digital densitometry.					
30348953	1	1	theme	low-friction	178:189	arg1	properties					191:200	low-friction properties	178:200	low-friction properties	178:200	Cartilage provides low-friction properties and plays an essential role in diarthrodial joints.					
30348953	8	2	theme	shear	1095:1099	arg1	strain					1101:1106	maximum shear strain	1087:1106	maximum shear strain	1087:1106	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	9	3	theme	degeneration	1283:1294	arg1	algorithm					1296:1304	the degeneration algorithm	1279:1304	the degeneration algorithm	1279:1304	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	10	4	theme	algorithm	1470:1478	arg1	predictions					1480:1490	Our degeneration algorithm predictions	1453:1490	Our degeneration algorithm predictions	1453:1490	Our degeneration algorithm predictions fitted well with the FCD content measured from the experiments.					
30348953	3	5	theme	tissue	527:532	arg1	composition					534:544	tissue composition	527:544	tissue composition	527:544	Unfortunately, traumatic joint loading can destroy this complex structure and produce lesions in tissue, leading later to changes in tissue composition and, ultimately, to post-traumatic osteoarthritis (PTOA).					
30348953	8	6	theme	novel	1007:1011	arg1	model					1036:1040	A novel cartilage degeneration model	1005:1040	A novel cartilage degeneration model	1005:1040	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	9	7	theme	discontinuous	1415:1427	arg1	loss					1433:1436	discontinuous FCD loss	1415:1436	discontinuous FCD loss around cracks	1415:1450	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	8	8	theme	FCD	1173:1175	arg1	loss					1177:1180	the FCD loss	1169:1180	the FCD loss	1169:1180	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	8	8	theme	FCD	1173:1175	arg1	function					1187:1194	a function	1185:1194	a function of time	1185:1202	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	7	9	theme	follow-up	942:950	arg1	points					957:962	different follow-up time points	932:962	different follow-up time points	932:962	FCD content at different follow-up time points was assessed using digital densitometry.					
30348953	6	10	theme	bovine	798:803	arg1	calves					805:810	bovine calves	798:810	bovine calves	798:810	Here, knee cartilage disks from bovine calves were injuriously compressed, followed by a physiologically relevant dynamic compression for twelve days.					
30348953	11	11	theme	depletion	1628:1636	arg1	prediction					1610:1619	prediction	1610:1619	prediction of FCD depletion around different cartilage lesions	1610:1671	The developed model could subsequently be applied for prediction of FCD depletion around different cartilage lesions and for suggesting optimal rehabilitation protocols.					
30348953	9	12	theme	FCD	1429:1431	arg1	loss					1433:1436	discontinuous FCD loss	1415:1436	discontinuous FCD loss around cracks	1415:1450	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	0	13	theme	proteoglycan	96:107	arg1	loss					109:112	proteoglycan loss	96:112	proteoglycan loss	96:112	A novel mechanobiological model can predict how physiologically relevant dynamic loading causes proteoglycan loss in mechanically injured articular cartilage.					
30348953	11	14	theme	rehabilitation	1700:1713	arg1	protocols					1715:1723	optimal rehabilitation protocols	1692:1723	optimal rehabilitation protocols	1692:1723	The developed model could subsequently be applied for prediction of FCD depletion around different cartilage lesions and for suggesting optimal rehabilitation protocols.					
30348953	7	15	from	points	957:962	arg1	content					921:927	FCD content	917:927	FCD content at different follow-up time points	917:962	FCD content at different follow-up time points was assessed using digital densitometry.					
30348953	7	16	theme	time	952:955	arg1	points					957:962	different follow-up time points	932:962	different follow-up time points	932:962	FCD content at different follow-up time points was assessed using digital densitometry.					
30348953	6	17	from	calves	805:810	arg1	disks					787:791	knee cartilage disks	772:791	knee cartilage disks from bovine calves	772:810	Here, knee cartilage disks from bovine calves were injuriously compressed, followed by a physiologically relevant dynamic compression for twelve days.					
30348953	5	18	theme	tissue	738:743	arg1	changes					745:751	these tissue changes	732:751	these tissue changes	732:751	However, the underlying mechanisms leading to these tissue changes are unknown.					
30348953	6	19	theme	dynamic	880:886	arg1	compression					888:898	a physiologically relevant dynamic compression	853:898	a physiologically relevant dynamic compression for twelve days	853:914	Here, knee cartilage disks from bovine calves were injuriously compressed, followed by a physiologically relevant dynamic compression for twelve days.					
30348953	9	20	theme	Predicted	1205:1213	arg1	loss					1215:1218	Predicted loss	1205:1218	Predicted loss of FCD	1205:1225	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	6	21	theme	cartilage	777:785	arg1	disks					787:791	knee cartilage disks	772:791	knee cartilage disks from bovine calves	772:810	Here, knee cartilage disks from bovine calves were injuriously compressed, followed by a physiologically relevant dynamic compression for twelve days.					
30348953	4	22	theme	PGs	652:654	arg1	FCD					644:646	FCD	644:646	FCD	644:646	Consequently, the fixed charge density (FCD) of PGs may decrease near the lesion.					
30348953	4	22	theme	PGs	652:654	arg1	density					635:641	the fixed charge density	618:641	the fixed charge density (FCD) of PGs	618:654	Consequently, the fixed charge density (FCD) of PGs may decrease near the lesion.					
30348953	3	23	theme	traumatic	409:417	arg1	loading					425:431	traumatic joint loading	409:431	traumatic joint loading	409:431	Unfortunately, traumatic joint loading can destroy this complex structure and produce lesions in tissue, leading later to changes in tissue composition and, ultimately, to post-traumatic osteoarthritis (PTOA).					
30348953	6	24	theme	relevant	871:878	arg1	compression					888:898	a physiologically relevant dynamic compression	853:898	a physiologically relevant dynamic compression for twelve days	853:914	Here, knee cartilage disks from bovine calves were injuriously compressed, followed by a physiologically relevant dynamic compression for twelve days.					
30348953	8	25	theme	maximum	1087:1093	arg1	strain					1101:1106	maximum shear strain	1087:1106	maximum shear strain	1087:1106	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	6	26	theme	knee	772:775	arg1	disks					787:791	knee cartilage disks	772:791	knee cartilage disks from bovine calves	772:810	Here, knee cartilage disks from bovine calves were injuriously compressed, followed by a physiologically relevant dynamic compression for twelve days.					
30348953	2	27	theme	collagen	337:344	arg1	network					346:352	a fibrillar collagen network	325:352	a fibrillar collagen network	325:352	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	27	theme	collagen	337:344	arg1	substance					272:280	A hydrated ground substance	254:280	A hydrated ground substance composed mainly of proteoglycans (PGs)	254:319	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	27	theme	collagen	337:344	arg1	constituents					367:378	the main constituents	358:378	the main constituents of cartilage	358:391	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	3	28	theme	complex	450:456	arg1	structure					458:466	this complex structure	445:466	this complex structure	445:466	Unfortunately, traumatic joint loading can destroy this complex structure and produce lesions in tissue, leading later to changes in tissue composition and, ultimately, to post-traumatic osteoarthritis (PTOA).					
30348953	3	29	theme	joint	419:423	arg1	loading					425:431	traumatic joint loading	409:431	traumatic joint loading	409:431	Unfortunately, traumatic joint loading can destroy this complex structure and produce lesions in tissue, leading later to changes in tissue composition and, ultimately, to post-traumatic osteoarthritis (PTOA).					
30348953	9	30	theme	fluid	1324:1328	arg1	velocity					1330:1337	the fluid velocity	1320:1337	the fluid velocity	1320:1337	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	10	31	theme	degeneration	1457:1468	arg1	predictions					1480:1490	Our degeneration algorithm predictions	1453:1490	Our degeneration algorithm predictions	1453:1490	Our degeneration algorithm predictions fitted well with the FCD content measured from the experiments.					
30348953	8	32	theme	degeneration	1023:1034	arg1	model					1036:1040	A novel cartilage degeneration model	1005:1040	A novel cartilage degeneration model	1005:1040	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	2	33	theme	fibrillar	327:335	arg1	network					346:352	a fibrillar collagen network	325:352	a fibrillar collagen network	325:352	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	33	theme	fibrillar	327:335	arg1	substance					272:280	A hydrated ground substance	254:280	A hydrated ground substance composed mainly of proteoglycans (PGs)	254:319	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	33	theme	fibrillar	327:335	arg1	constituents					367:378	the main constituents	358:378	the main constituents of cartilage	358:391	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	11	34	theme	cartilage	1655:1663	arg1	lesions					1665:1671	different cartilage lesions	1645:1671	different cartilage lesions	1645:1671	The developed model could subsequently be applied for prediction of FCD depletion around different cartilage lesions and for suggesting optimal rehabilitation protocols.					
30348953	1	35	theme	essential	215:223	arg1	role					225:228	an essential role	212:228	an essential role	212:228	Cartilage provides low-friction properties and plays an essential role in diarthrodial joints.					
30348953	8	36	theme	time	1199:1202	arg1	loss					1177:1180	the FCD loss	1169:1180	the FCD loss	1169:1180	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	8	36	theme	time	1199:1202	arg1	function					1187:1194	a function	1185:1194	a function of time	1185:1202	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	11	37	theme	optimal	1692:1698	arg1	protocols					1715:1723	optimal rehabilitation protocols	1692:1723	optimal rehabilitation protocols	1692:1723	The developed model could subsequently be applied for prediction of FCD depletion around different cartilage lesions and for suggesting optimal rehabilitation protocols.					
30348953	0	38	theme	articular	138:146	arg1	cartilage					148:156	mechanically injured articular cartilage	117:156	mechanically injured articular cartilage	117:156	A novel mechanobiological model can predict how physiologically relevant dynamic loading causes proteoglycan loss in mechanically injured articular cartilage.					
30348953	3	39	from	changes	516:522	arg1	composition					534:544	tissue composition	527:544	tissue composition	527:544	Unfortunately, traumatic joint loading can destroy this complex structure and produce lesions in tissue, leading later to changes in tissue composition and, ultimately, to post-traumatic osteoarthritis (PTOA).					
30348953	3	40	theme	post-traumatic	566:579	arg1	osteoarthritis					581:594	post-traumatic osteoarthritis	566:594	post-traumatic osteoarthritis (PTOA)	566:601	Unfortunately, traumatic joint loading can destroy this complex structure and produce lesions in tissue, leading later to changes in tissue composition and, ultimately, to post-traumatic osteoarthritis (PTOA).					
30348953	3	40	theme	post-traumatic	566:579	arg1	PTOA					597:600	PTOA	597:600	PTOA	597:600	Unfortunately, traumatic joint loading can destroy this complex structure and produce lesions in tissue, leading later to changes in tissue composition and, ultimately, to post-traumatic osteoarthritis (PTOA).					
30348953	0	41	theme	mechanobiological	8:24	arg1	model					26:30	A novel mechanobiological model	0:30	A novel mechanobiological model	0:30	A novel mechanobiological model can predict how physiologically relevant dynamic loading causes proteoglycan loss in mechanically injured articular cartilage.					
30348953	0	42	theme	injured	130:136	arg1	cartilage					148:156	mechanically injured articular cartilage	117:156	mechanically injured articular cartilage	117:156	A novel mechanobiological model can predict how physiologically relevant dynamic loading causes proteoglycan loss in mechanically injured articular cartilage.					
30348953	9	43	theme	FCD	1223:1225	arg1	loss					1215:1218	Predicted loss	1205:1218	Predicted loss of FCD	1205:1225	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	6	44	dep	compressed	829:838	arg1	followed					841:848	followed	841:848	followed by a physiologically relevant dynamic compression for twelve days	841:914	Here, knee cartilage disks from bovine calves were injuriously compressed, followed by a physiologically relevant dynamic compression for twelve days.					
30348953	5	45	theme	underlying	699:708	arg1	unknown					757:763	unknown	757:763	unknown	757:763	However, the underlying mechanisms leading to these tissue changes are unknown.					
30348953	5	45	theme	underlying	699:708	arg1	mechanisms					710:719	the underlying mechanisms	695:719	the underlying mechanisms leading to these tissue changes	695:751	However, the underlying mechanisms leading to these tissue changes are unknown.					
30348953	2	46	theme	ground	265:270	arg1	network					346:352	a fibrillar collagen network	325:352	a fibrillar collagen network	325:352	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	46	theme	ground	265:270	arg1	constituents					367:378	the main constituents	358:378	the main constituents of cartilage	358:391	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	46	theme	ground	265:270	arg1	substance					272:280	A hydrated ground substance	254:280	A hydrated ground substance composed mainly of proteoglycans (PGs)	254:319	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	1	47	theme	diarthrodial	233:244	arg1	joints					246:251	diarthrodial joints	233:251	diarthrodial joints	233:251	Cartilage provides low-friction properties and plays an essential role in diarthrodial joints.					
30348953	2	48	theme	hydrated	256:263	arg1	network					346:352	a fibrillar collagen network	325:352	a fibrillar collagen network	325:352	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	48	theme	hydrated	256:263	arg1	constituents					367:378	the main constituents	358:378	the main constituents of cartilage	358:391	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	48	theme	hydrated	256:263	arg1	substance					272:280	A hydrated ground substance	254:280	A hydrated ground substance composed mainly of proteoglycans (PGs)	254:319	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	3	49	from	lesions	480:486	arg1	tissue					491:496	tissue	491:496	tissue	491:496	Unfortunately, traumatic joint loading can destroy this complex structure and produce lesions in tissue, leading later to changes in tissue composition and, ultimately, to post-traumatic osteoarthritis (PTOA).					
30348953	9	50	theme	shear	1365:1369	arg1	strain					1371:1376	shear strain	1365:1376	shear strain	1365:1376	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	8	51	theme	controlled	1135:1144	arg1	algorithms					1146:1155	controlled algorithms	1135:1155	controlled algorithms	1135:1155	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	2	52	theme	main	362:365	arg1	network					346:352	a fibrillar collagen network	325:352	a fibrillar collagen network	325:352	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	52	theme	main	362:365	arg1	substance					272:280	A hydrated ground substance	254:280	A hydrated ground substance composed mainly of proteoglycans (PGs)	254:319	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	52	theme	main	362:365	arg1	constituents					367:378	the main constituents	358:378	the main constituents of cartilage	358:391	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	10	53	theme	FCD	1513:1515	arg1	content					1517:1523	the FCD content	1509:1523	the FCD content measured from the experiments	1509:1553	Our degeneration algorithm predictions fitted well with the FCD content measured from the experiments.					
30348953	8	54	theme	cartilage	1013:1021	arg1	model					1036:1040	A novel cartilage degeneration model	1005:1040	A novel cartilage degeneration model	1005:1040	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	7	55	theme	different	932:940	arg1	points					957:962	different follow-up time points	932:962	different follow-up time points	932:962	FCD content at different follow-up time points was assessed using digital densitometry.					
30348953	11	56	theme	developed	1560:1568	arg1	model					1570:1574	The developed model	1556:1574	The developed model	1556:1574	The developed model could subsequently be applied for prediction of FCD depletion around different cartilage lesions and for suggesting optimal rehabilitation protocols.					
30348953	7	57	theme	FCD	917:919	arg1	content					921:927	FCD content	917:927	FCD content at different follow-up time points	917:962	FCD content at different follow-up time points was assessed using digital densitometry.					
30348953	4	58	theme	fixed	622:626	arg1	FCD					644:646	FCD	644:646	FCD	644:646	Consequently, the fixed charge density (FCD) of PGs may decrease near the lesion.					
30348953	4	58	theme	fixed	622:626	arg1	density					635:641	the fixed charge density	618:641	the fixed charge density (FCD) of PGs	618:654	Consequently, the fixed charge density (FCD) of PGs may decrease near the lesion.					
30348953	8	59	theme	fluid	1120:1124	arg1	velocity					1126:1133	fluid velocity	1120:1133	fluid velocity	1120:1133	A novel cartilage degeneration model was developed by implementing deviatoric and maximum shear strain, as well as fluid velocity controlled algorithms to simulate the FCD loss as a function of time.					
30348953	9	60	theme	cartilage	1256:1264	arg1	lesions					1266:1272	the cartilage lesions	1252:1272	the cartilage lesions	1252:1272	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	2	61	theme	cartilage	383:391	arg1	network					346:352	a fibrillar collagen network	325:352	a fibrillar collagen network	325:352	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	61	theme	cartilage	383:391	arg1	substance					272:280	A hydrated ground substance	254:280	A hydrated ground substance composed mainly of proteoglycans (PGs)	254:319	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	2	61	theme	cartilage	383:391	arg1	constituents					367:378	the main constituents	358:378	the main constituents of cartilage	358:391	A hydrated ground substance composed mainly of proteoglycans (PGs) and a fibrillar collagen network are the main constituents of cartilage.					
30348953	9	62	theme	driven	1378:1383	arg1	mechanisms					1385:1394	driven mechanisms	1378:1394	driven mechanisms	1378:1394	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	0	63	theme	dynamic	73:79	arg1	loading					81:87	physiologically relevant dynamic loading	48:87	physiologically relevant dynamic loading	48:87	A novel mechanobiological model can predict how physiologically relevant dynamic loading causes proteoglycan loss in mechanically injured articular cartilage.					
30348953	11	64	theme	FCD	1624:1626	arg1	depletion					1628:1636	FCD depletion	1624:1636	FCD depletion	1624:1636	The developed model could subsequently be applied for prediction of FCD depletion around different cartilage lesions and for suggesting optimal rehabilitation protocols.					
30348953	11	65	theme	different	1645:1653	arg1	lesions					1665:1671	different cartilage lesions	1645:1671	different cartilage lesions	1645:1671	The developed model could subsequently be applied for prediction of FCD depletion around different cartilage lesions and for suggesting optimal rehabilitation protocols.					
30348953	0	66	theme	relevant	64:71	arg1	loading					81:87	physiologically relevant dynamic loading	48:87	physiologically relevant dynamic loading	48:87	A novel mechanobiological model can predict how physiologically relevant dynamic loading causes proteoglycan loss in mechanically injured articular cartilage.					
30348953	9	67	dep	deviatoric	1350:1359	arg1	mechanisms					1385:1394	driven mechanisms	1378:1394	driven mechanisms	1378:1394	Predicted loss of FCD was quite uniform around the cartilage lesions when the degeneration algorithm was driven by the fluid velocity, while the deviatoric and shear strain driven mechanisms exhibited slightly discontinuous FCD loss around cracks.					
30348953	0	68	theme	novel	2:6	arg1	model					26:30	A novel mechanobiological model	0:30	A novel mechanobiological model	0:30	A novel mechanobiological model can predict how physiologically relevant dynamic loading causes proteoglycan loss in mechanically injured articular cartilage.					
30348953	4	69	theme	charge	628:633	arg1	FCD					644:646	FCD	644:646	FCD	644:646	Consequently, the fixed charge density (FCD) of PGs may decrease near the lesion.					
30348953	4	69	theme	charge	628:633	arg1	density					635:641	the fixed charge density	618:641	the fixed charge density (FCD) of PGs	618:654	Consequently, the fixed charge density (FCD) of PGs may decrease near the lesion.					
29243576	0	0	theme	Physicochemical	89:103	arg1	Properties					105:114	Topotecan Hydrochloride Physicochemical Properties	65:114	Topotecan Hydrochloride Physicochemical Properties	65:114	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.					
29243576	6	1	theme	microemulsion	912:924	arg1	technique					926:934	a microemulsion technique	910:934	a microemulsion technique	910:934	TPT-PLN was then obtained via a microemulsion technique, and particle size, zeta potential, encapsulation efficiency, drug loading and drug recovery were determined.					
29243576	13	2	theme	TPT	1794:1796	arg1	loading					1798:1804	TPT loading	1794:1804	TPT loading	1794:1804	CONCLUSION PLN has shown to be a more effective nanosystem for TPT nanoencapsulation because TPT loading, stability and release were superior when compared to TPT-NLC.					
29243576	1	3	theme	stability	262:270	arg1	loss					272:275	stability loss	262:275	stability loss	262:275	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	0	4	theme	Hydrochloride	75:87	arg1	Properties					105:114	Topotecan Hydrochloride Physicochemical Properties	65:114	Topotecan Hydrochloride Physicochemical Properties	65:114	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.					
29243576	2	5	theme	hybrid	372:377	arg1	nanoparticles					379:391	polymer-lipid hybrid nanoparticles	358:391	polymer-lipid hybrid nanoparticles (PLN)	358:397	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	2	5	theme	hybrid	372:377	arg1	alternatives					415:426	interesting alternatives	403:426	interesting alternatives	403:426	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	2	5	theme	hybrid	372:377	arg1	PLN					394:396	PLN	394:396	PLN	394:396	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	13	6	theme	effective	1739:1747	arg1	nanosystem					1749:1758	a more effective nanosystem	1732:1758	a more effective nanosystem for TPT nanoencapsulation	1732:1784	CONCLUSION PLN has shown to be a more effective nanosystem for TPT nanoencapsulation because TPT loading, stability and release were superior when compared to TPT-NLC.					
29243576	13	6	theme	effective	1739:1747	arg1	PLN					1712:1714	CONCLUSION PLN	1701:1714	CONCLUSION PLN	1701:1714	CONCLUSION PLN has shown to be a more effective nanosystem for TPT nanoencapsulation because TPT loading, stability and release were superior when compared to TPT-NLC.					
29243576	7	7	dep	in	1082:1083	arg1	vitro					1085:1089	vitro	1085:1089	vitro	1085:1089	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	10	8	theme	encapsulation	1410:1422	arg1	efficiency					1424:1433	high encapsulation efficiency	1405:1433	high encapsulation efficiency (97%)	1405:1439	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	10	8	theme	encapsulation	1410:1422	arg1	%					1438:1438	97%	1436:1438	97%	1436:1438	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	2	9	theme	Lipid	303:307	arg1	nanoencapsulation					309:325	Lipid nanoencapsulation	303:325	Lipid nanoencapsulation	303:325	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	4	10	from	ratio	694:698	arg1	complexation					710:721	DS-TPT complexation	703:721	DS-TPT complexation	703:721	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	0	11	theme	Properties	105:114	arg1	Improvement					50:60	the Improvement	46:60	the Improvement of Topotecan Hydrochloride Physicochemical Properties	46:114	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.					
29243576	13	12	theme	CONCLUSION	1701:1710	arg1	PLN					1712:1714	CONCLUSION PLN	1701:1714	CONCLUSION PLN	1701:1714	CONCLUSION PLN has shown to be a more effective nanosystem for TPT nanoencapsulation because TPT loading, stability and release were superior when compared to TPT-NLC.					
29243576	13	12	theme	CONCLUSION	1701:1710	arg1	nanosystem					1749:1758	a more effective nanosystem	1732:1758	a more effective nanosystem for TPT nanoencapsulation	1732:1784	CONCLUSION PLN has shown to be a more effective nanosystem for TPT nanoencapsulation because TPT loading, stability and release were superior when compared to TPT-NLC.					
29243576	10	13	theme	high	1405:1408	arg1	efficiency					1424:1433	high encapsulation efficiency	1405:1433	high encapsulation efficiency (97%)	1405:1439	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	10	13	theme	high	1405:1408	arg1	%					1438:1438	97%	1436:1438	97%	1436:1438	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	11	14	theme	nm	1568:1569	arg1	diameter					1511:1518	a mean diameter	1504:1518	a mean diameter	1504:1518	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	14	theme	nm	1568:1569	arg1	mV					1584:1585	0.2 and -22 mV	1572:1585	0.2 and -22 mV	1572:1585	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	14	theme	nm	1568:1569	arg1	potential					1551:1559	polidispersivity index e zeta potential	1521:1559	polidispersivity index e zeta potential of 140 nm	1521:1569	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	1	15	theme	poor	281:284	arg1	uptake					295:300	poor cellular uptake	281:300	poor cellular uptake	281:300	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	12	16	theme	chemical	1610:1617	arg1	stability					1619:1627	The TPT chemical stability	1602:1627	The TPT chemical stability	1602:1627	The TPT chemical stability and release from PLN were observed to be superior when compared to NLC.					
29243576	12	16	theme	chemical	1610:1617	arg1	superior					1670:1677	superior	1670:1677	superior	1670:1677	The TPT chemical stability and release from PLN were observed to be superior when compared to NLC.					
29243576	3	17	theme	cationic	522:529	arg1	TPT					531:533	the cationic TPT	518:533	the cationic TPT	518:533	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	4	18	theme	selected	747:754	arg1	complex					756:762	the selected complex	743:762	the selected complex	743:762	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	3	19	theme	dextran	562:568	arg1	DS					579:580	DS	579:580	DS	579:580	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	3	19	theme	dextran	562:568	arg1	sulfate					570:576	the negatively charged dextran sulfate	539:576	the negatively charged dextran sulfate (DS)	539:581	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	13	20	theme	TPT	1764:1766	arg1	nanoencapsulation					1768:1784	TPT nanoencapsulation	1764:1784	TPT nanoencapsulation	1764:1784	CONCLUSION PLN has shown to be a more effective nanosystem for TPT nanoencapsulation because TPT loading, stability and release were superior when compared to TPT-NLC.					
29243576	1	21	theme	camptothecin	175:186	arg1	derivate					163:170	a water-soluble derivate	147:170	a water-soluble derivate	147:170	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	1	21	theme	camptothecin	175:186	arg1	Topotecan					128:136	BACKGROUND Topotecan	117:136	BACKGROUND Topotecan (TPT)	117:142	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	6	22	theme	encapsulation	972:984	arg1	efficiency					986:995	encapsulation efficiency	972:995	encapsulation efficiency	972:995	TPT-PLN was then obtained via a microemulsion technique, and particle size, zeta potential, encapsulation efficiency, drug loading and drug recovery were determined.					
29243576	5	23	theme	TPT	815:817	arg1	solubility					819:828	TPT solubility	815:828	TPT solubility in the free and complexed forms	815:860	TPT solubility in the free and complexed forms was also assayed.					
29243576	4	24	theme	FTIR	785:788	arg1	NMR					810:812	FTIR and solid-state 13C NMR	785:812	NMR	810:812	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	2	25	theme	interesting	403:413	arg1	nanoparticles					379:391	polymer-lipid hybrid nanoparticles	358:391	polymer-lipid hybrid nanoparticles (PLN)	358:397	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	2	25	theme	interesting	403:413	arg1	alternatives					415:426	interesting alternatives	403:426	interesting alternatives	403:426	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	0	26	theme	Novel	2:6	arg1	Nanoparticle					29:40	A Novel Polymer-Lipid Hybrid Nanoparticle	0:40	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.	0:115	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.					
29243576	5	27	from	solubility	819:828	arg1	forms					856:860	the free and complexed forms	833:860	the free and complexed forms	833:860	TPT solubility in the free and complexed forms was also assayed.					
29243576	7	28	theme	lipid	1206:1210	arg1	NLC					1222:1224	NLC	1222:1224	NLC	1222:1224	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	7	28	theme	lipid	1206:1210	arg1	carriers					1212:1219	nanostructured lipid carriers	1191:1219	nanostructured lipid carriers (NLC) of similar composition	1191:1248	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	9	29	theme	TPT	1310:1312	arg1	solubility					1314:1323	TPT solubility	1310:1323	TPT solubility in the complex	1310:1338	TPT solubility in the complex was drastically decreased when compared to free TPT.					
29243576	2	30	theme	TPT	339:341	arg1	stability					343:351	TPT stability	339:351	TPT stability	339:351	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	7	31	theme	TPT-DS	1152:1157	arg1	complex					1159:1165	TPT-DS complex	1152:1165	TPT-DS complex	1152:1165	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	4	32	theme	molar	688:692	arg1	ratio					694:698	The optimum ionic molar ratio	670:698	METHODS The optimum ionic molar ratio in DS-TPT complexation	662:721	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	1	33	theme	cellular	286:293	arg1	uptake					295:300	poor cellular uptake	281:300	poor cellular uptake	281:300	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	7	34	theme	in	1082:1083	arg1	release					1091:1097	in vitro release	1082:1097	in vitro release	1082:1097	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	6	35	theme	drug	998:1001	arg1	loading					1003:1009	drug loading	998:1009	drug loading	998:1009	TPT-PLN was then obtained via a microemulsion technique, and particle size, zeta potential, encapsulation efficiency, drug loading and drug recovery were determined.					
29243576	4	36	theme	ionic	682:686	arg1	ratio					694:698	The optimum ionic molar ratio	670:698	METHODS The optimum ionic molar ratio in DS-TPT complexation	662:721	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	0	37	theme	Hybrid	22:27	arg1	Nanoparticle					29:40	A Novel Polymer-Lipid Hybrid Nanoparticle	0:40	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.	0:115	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.					
29243576	7	38	theme	TPT	1064:1066	arg1	stability					1068:1076	the TPT stability	1060:1076	the TPT stability	1060:1076	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	11	39	theme	mean	1506:1509	arg1	diameter					1511:1518	a mean diameter	1504:1518	a mean diameter	1504:1518	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	39	theme	mean	1506:1509	arg1	potential					1551:1559	polidispersivity index e zeta potential	1521:1559	polidispersivity index e zeta potential of 140 nm	1521:1569	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	1	40	theme	BACKGROUND	117:126	arg1	derivate					163:170	a water-soluble derivate	147:170	a water-soluble derivate	147:170	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	1	40	theme	BACKGROUND	117:126	arg1	TPT					139:141	TPT	139:141	TPT	139:141	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	1	40	theme	BACKGROUND	117:126	arg1	Topotecan					128:136	BACKGROUND Topotecan	117:136	BACKGROUND Topotecan (TPT)	117:142	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	4	41	dep	METHODS	662:668	arg1	ratio					694:698	The optimum ionic molar ratio	670:698	METHODS The optimum ionic molar ratio in DS-TPT complexation	662:721	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	1	42	theme	ring-opening	205:216	arg1	hydrolysis					218:227	ring-opening hydrolysis	205:227	ring-opening hydrolysis in neutral solutions	205:248	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	0	43	theme	Polymer-Lipid	8:20	arg1	Nanoparticle					29:40	A Novel Polymer-Lipid Hybrid Nanoparticle	0:40	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.	0:115	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.					
29243576	6	44	theme	zeta	956:959	arg1	potential					961:969	zeta potential	956:969	zeta potential	956:969	TPT-PLN was then obtained via a microemulsion technique, and particle size, zeta potential, encapsulation efficiency, drug loading and drug recovery were determined.					
29243576	11	45	theme	e	1544:1544	arg1	diameter					1511:1518	a mean diameter	1504:1518	a mean diameter	1504:1518	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	45	theme	e	1544:1544	arg1	mV					1584:1585	0.2 and -22 mV	1572:1585	0.2 and -22 mV	1572:1585	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	45	theme	e	1544:1544	arg1	potential					1551:1559	polidispersivity index e zeta potential	1521:1559	polidispersivity index e zeta potential of 140 nm	1521:1569	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	4	46	theme	DS-TPT	703:708	arg1	complexation					710:721	DS-TPT complexation	703:721	DS-TPT complexation	703:721	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	7	47	theme	free	1142:1145	arg1	TPT					1147:1149	free TPT	1142:1149	free TPT	1142:1149	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	2	48	theme	TPT	439:441	arg1	nanoencapsulation					443:459	TPT nanoencapsulation	439:459	TPT nanoencapsulation	439:459	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	10	49	theme	loading	1450:1456	arg1	capacity					1458:1465	drug loading capacity	1445:1465	drug loading capacity (5.5%)	1445:1472	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	10	49	theme	loading	1450:1456	arg1	%					1471:1471	5.5%	1468:1471	5.5%	1468:1471	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	8	50	theme	TPT-DS	1259:1264	arg1	complexation					1266:1277	RESULTS TPT-DS complexation	1251:1277	RESULTS TPT-DS complexation	1251:1277	RESULTS TPT-DS complexation was confirmed by FTIR and NMR.					
29243576	3	51	theme	drug	608:611	arg1	loading					613:619	drug loading	608:619	drug loading	608:619	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	5	52	theme	complexed	846:854	arg1	forms					856:860	the free and complexed forms	833:860	the free and complexed forms	833:860	TPT solubility in the free and complexed forms was also assayed.					
29243576	4	53	theme	optimum	674:680	arg1	ratio					694:698	The optimum ionic molar ratio	670:698	METHODS The optimum ionic molar ratio in DS-TPT complexation	662:721	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	3	54	with	complexes	500:508	arg1	view					590:593	a view	588:593	a view of improving drug loading, chemical stability and release control	588:659	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	3	55	theme	release	645:651	arg1	control					653:659	release control	645:659	release control	645:659	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	3	56	dep	OBJECTIVE	462:470	arg1	seeks					483:487	seeks	483:487	seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control	483:659	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	9	57	from	solubility	1314:1323	arg1	complex					1332:1338	the complex	1328:1338	the complex	1328:1338	TPT solubility in the complex was drastically decreased when compared to free TPT.					
29243576	1	58	theme	neutral	232:238	arg1	solutions					240:248	neutral solutions	232:248	neutral solutions	232:248	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	6	59	theme	particle	941:948	arg1	size					950:953	particle size	941:953	particle size	941:953	TPT-PLN was then obtained via a microemulsion technique, and particle size, zeta potential, encapsulation efficiency, drug loading and drug recovery were determined.					
29243576	11	60	theme	zeta	1546:1549	arg1	diameter					1511:1518	a mean diameter	1504:1518	a mean diameter	1504:1518	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	60	theme	zeta	1546:1549	arg1	mV					1584:1585	0.2 and -22 mV	1572:1585	0.2 and -22 mV	1572:1585	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	60	theme	zeta	1546:1549	arg1	potential					1551:1559	polidispersivity index e zeta potential	1521:1559	polidispersivity index e zeta potential of 140 nm	1521:1569	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	2	61	theme	polymer-lipid	358:370	arg1	nanoparticles					379:391	polymer-lipid hybrid nanoparticles	358:391	polymer-lipid hybrid nanoparticles (PLN)	358:397	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	2	61	theme	polymer-lipid	358:370	arg1	alternatives					415:426	interesting alternatives	403:426	interesting alternatives	403:426	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	2	61	theme	polymer-lipid	358:370	arg1	PLN					394:396	PLN	394:396	PLN	394:396	Lipid nanoencapsulation can improve TPT stability, and polymer-lipid hybrid nanoparticles (PLN) are interesting alternatives to improve TPT nanoencapsulation.					
29243576	10	62	theme	drug	1445:1448	arg1	capacity					1458:1465	drug loading capacity	1445:1465	drug loading capacity (5.5%)	1445:1472	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	10	62	theme	drug	1445:1448	arg1	%					1471:1471	5.5%	1468:1471	5.5%	1468:1471	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	10	63	contain	had	1401:1403	arg2	%					1438:1438	97%	1436:1438	97%	1436:1438	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	10	63	contain	had	1401:1403	arg2	capacity					1458:1465	drug loading capacity	1445:1465	drug loading capacity (5.5%)	1445:1472	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	10	63	contain	had	1401:1403	arg1	TPT-PLN					1393:1399	TPT-PLN	1393:1399	TPT-PLN	1393:1399	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	10	63	contain	had	1401:1403	arg2	%					1471:1471	5.5%	1468:1471	5.5%	1468:1471	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	10	63	contain	had	1401:1403	arg2	efficiency					1424:1433	high encapsulation efficiency	1405:1433	high encapsulation efficiency (97%)	1405:1439	TPT-PLN had high encapsulation efficiency (97%) and drug loading capacity (5.5%).					
29243576	11	64	theme	polidispersivity	1521:1536	arg1	diameter					1511:1518	a mean diameter	1504:1518	a mean diameter	1504:1518	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	64	theme	polidispersivity	1521:1536	arg1	mV					1584:1585	0.2 and -22 mV	1572:1585	0.2 and -22 mV	1572:1585	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	64	theme	polidispersivity	1521:1536	arg1	potential					1551:1559	polidispersivity index e zeta potential	1521:1559	polidispersivity index e zeta potential of 140 nm	1521:1569	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	7	65	theme	similar	1230:1236	arg1	composition					1238:1248	similar composition	1230:1248	similar composition	1230:1248	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	4	66	theme	13C	806:808	arg1	NMR					810:812	FTIR and solid-state 13C NMR	785:812	NMR	810:812	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	1	67	from	hydrolysis	218:227	arg1	solutions					240:248	neutral solutions	232:248	neutral solutions	232:248	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	7	68	theme	composition	1238:1248	arg1	NLC					1222:1224	NLC	1222:1224	NLC	1222:1224	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	7	68	theme	composition	1238:1248	arg1	carriers					1212:1219	nanostructured lipid carriers	1191:1219	nanostructured lipid carriers (NLC) of similar composition	1191:1248	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	7	69	theme	nanostructured	1191:1204	arg1	NLC					1222:1224	NLC	1222:1224	NLC	1222:1224	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	7	69	theme	nanostructured	1191:1204	arg1	carriers					1212:1219	nanostructured lipid carriers	1191:1219	nanostructured lipid carriers (NLC) of similar composition	1191:1248	Additionally, the TPT stability and in vitro release were determined from PLN and compared with free TPT, TPT-DS complex and TPT encapsulated in nanostructured lipid carriers (NLC) of similar composition.					
29243576	4	70	theme	solid-state	794:804	arg1	NMR					810:812	FTIR and solid-state 13C NMR	785:812	NMR	810:812	METHODS The optimum ionic molar ratio in DS-TPT complexation was determined, and the selected complex was characterized by FTIR and solid-state 13C NMR.					
29243576	3	71	theme	charged	554:560	arg1	DS					579:580	DS	579:580	DS	579:580	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	3	71	theme	charged	554:560	arg1	sulfate					570:576	the negatively charged dextran sulfate	539:576	the negatively charged dextran sulfate (DS)	539:581	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	12	72	from	PLN	1646:1648	arg1	release					1633:1639	release	1633:1639	release from PLN	1633:1648	The TPT chemical stability and release from PLN were observed to be superior when compared to NLC.					
29243576	12	72	from	PLN	1646:1648	arg1	stability					1619:1627	The TPT chemical stability	1602:1627	The TPT chemical stability	1602:1627	The TPT chemical stability and release from PLN were observed to be superior when compared to NLC.					
29243576	12	72	from	PLN	1646:1648	arg1	superior					1670:1677	superior	1670:1677	superior	1670:1677	The TPT chemical stability and release from PLN were observed to be superior when compared to NLC.					
29243576	6	73	theme	drug	1015:1018	arg1	recovery					1020:1027	drug recovery	1015:1027	drug recovery	1015:1027	TPT-PLN was then obtained via a microemulsion technique, and particle size, zeta potential, encapsulation efficiency, drug loading and drug recovery were determined.					
29243576	0	74	theme	Topotecan	65:73	arg1	Properties					105:114	Topotecan Hydrochloride Physicochemical Properties	65:114	Topotecan Hydrochloride Physicochemical Properties	65:114	A Novel Polymer-Lipid Hybrid Nanoparticle for the Improvement of Topotecan Hydrochloride Physicochemical Properties.					
29243576	8	75	theme	RESULTS	1251:1257	arg1	complexation					1266:1277	RESULTS TPT-DS complexation	1251:1277	RESULTS TPT-DS complexation	1251:1277	RESULTS TPT-DS complexation was confirmed by FTIR and NMR.					
29243576	5	76	theme	free	837:840	arg1	forms					856:860	the free and complexed forms	833:860	the free and complexed forms	833:860	TPT solubility in the free and complexed forms was also assayed.					
29243576	11	77	theme	index	1538:1542	arg1	diameter					1511:1518	a mean diameter	1504:1518	a mean diameter	1504:1518	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	77	theme	index	1538:1542	arg1	mV					1584:1585	0.2 and -22 mV	1572:1585	0.2 and -22 mV	1572:1585	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	11	77	theme	index	1538:1542	arg1	potential					1551:1559	polidispersivity index e zeta potential	1521:1559	polidispersivity index e zeta potential of 140 nm	1521:1569	Additionally, TPT-PLN showed a mean diameter, polidispersivity index e zeta potential of 140 nm, 0.2 and -22 mV, respectively.					
29243576	3	78	theme	chemical	622:629	arg1	stability					631:639	chemical stability	622:639	chemical stability	622:639	OBJECTIVE This study seeks to prepare complexes between the cationic TPT and the negatively charged dextran sulfate (DS) with a view of improving drug loading, chemical stability and release control.					
29243576	1	79	theme	water-soluble	149:161	arg1	derivate					163:170	a water-soluble derivate	147:170	a water-soluble derivate	147:170	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	1	79	theme	water-soluble	149:161	arg1	Topotecan					128:136	BACKGROUND Topotecan	117:136	BACKGROUND Topotecan (TPT)	117:142	BACKGROUND Topotecan (TPT) is a water-soluble derivate of camptothecin, which undergoes ring-opening hydrolysis in neutral solutions, leading to stability loss and poor cellular uptake.					
29243576	12	80	theme	TPT	1606:1608	arg1	stability					1619:1627	The TPT chemical stability	1602:1627	The TPT chemical stability	1602:1627	The TPT chemical stability and release from PLN were observed to be superior when compared to NLC.					
29243576	12	80	theme	TPT	1606:1608	arg1	superior					1670:1677	superior	1670:1677	superior	1670:1677	The TPT chemical stability and release from PLN were observed to be superior when compared to NLC.					
29243576	9	81	theme	free	1383:1386	arg1	TPT					1388:1390	free TPT	1383:1390	free TPT	1383:1390	TPT solubility in the complex was drastically decreased when compared to free TPT.					
31681475	8	0	theme	water	2478:2482	arg1	content					2484:2490	higher structural water content	2460:2490	higher structural water content	2460:2490	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	0	theme	water	2478:2482	arg1	surface					2448:2454	its possibly less stable and more diffusive particle surface	2395:2454	its possibly less stable and more diffusive particle surface	2395:2454	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	0	theme	water	2478:2482	arg1	retention					2201:2209	the retention	2197:2209	the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state	2197:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	0	theme	water	2478:2482	arg1	reasons					2521:2527	potential reasons	2511:2527	potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation	2511:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	7	1	theme	polymeric	1877:1885	arg1	components					1887:1896	polymeric components	1877:1896	polymeric components to HAp	1877:1903	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	7	1	theme	polymeric	1877:1885	arg1	addition					1842:1849	the addition	1838:1849	the addition of chitosan or gelatin	1838:1872	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	7	2	theme	gelatin	1866:1872	arg1	components					1887:1896	polymeric components	1877:1896	polymeric components to HAp	1877:1903	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	7	2	theme	gelatin	1866:1872	arg1	addition					1842:1849	the addition	1838:1849	the addition of chitosan or gelatin	1838:1872	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	3	3	theme	different	636:644	arg1	ratios					646:651	different ratios	636:651	different ratios	636:651	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	1	4	theme	release	167:173	arg1	kinetics					150:157	The kinetics	146:157	The kinetics of drug release from hydroxyapatite (HAp)	146:199	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	7	5	theme	chitosan	1854:1861	arg1	components					1887:1896	polymeric components	1877:1896	polymeric components to HAp	1877:1903	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	7	5	theme	chitosan	1854:1861	arg1	addition					1842:1849	the addition	1838:1849	the addition of chitosan or gelatin	1838:1872	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	3	6	theme	release	672:678	arg1	kinetics					680:687	their drug release kinetics	661:687	their drug release kinetics	661:687	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	8	7	theme	concentration	2223:2235	arg1	content					2484:2490	higher structural water content	2460:2490	higher structural water content	2460:2490	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	7	theme	concentration	2223:2235	arg1	surface					2448:2454	its possibly less stable and more diffusive particle surface	2395:2454	its possibly less stable and more diffusive particle surface	2395:2454	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	7	theme	concentration	2223:2235	arg1	retention					2201:2209	the retention	2197:2209	the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state	2197:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	7	theme	concentration	2223:2235	arg1	reasons					2521:2527	potential reasons	2511:2527	potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation	2511:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	6	8	theme	tunable	1731:1737	arg1	release					1739:1745	a universally tunable release	1717:1745	a universally tunable release of drugs based on the passive transport	1717:1785	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	8	9	theme	slit-like	2240:2248	arg1	pores					2250:2254	slit-like pores	2240:2254	slit-like pores	2240:2254	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	0	10	theme	drug-dependent	123:136	arg1	manner					138:143	a drug-dependent manner	121:143	a drug-dependent manner	121:143	Mechanism of formation governs the mechanism of release of antibiotics from calcium phosphate nanopowders and cements in a drug-dependent manner.					
31681475	8	11	theme	amorphous	2263:2271	arg1	precursor					2273:2281	the amorphous precursor	2259:2281	the amorphous precursor in HAp2 and its lower surface energy	2259:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	7	12	theme	Preliminary	1809:1819	arg1	assays					1821:1826	Preliminary assays	1809:1826	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp	1809:1903	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	4	13	theme	amorphous	835:843	arg1	phase					845:849	the amorphous phase	831:849	the amorphous phase during gelation (HAp2)	831:872	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	8	14	from	mechanisms	2563:2572	arg1	powders					2602:2608	the two HAp powders	2590:2608	the two HAp powders with different histories of formation	2590:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	5	15	from	increase	1362:1369	arg1	content					1378:1384	the content	1374:1384	the content of the cement component retaining its crystallinity during gelation	1374:1452	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	4	16	theme	Fickian	1099:1105	arg1	case					1117:1120	Fickian diffusion case	1099:1120	Fickian diffusion case	1099:1120	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	5	17	theme	effect	1196:1201	arg1	got					1203:1205	This effect got	1191:1205	This effect got	1191:1205	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	8	18	theme	binding	2336:2342	arg1	potential					2344:2352	lesser drug binding potential	2324:2352	lesser drug binding potential	2324:2352	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	3	19	theme	release	708:714	arg1	mechanism					716:724	the release mechanism	704:724	the release mechanism	704:724	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	4	20	theme	anomalous	908:916	arg1	mechanism					931:939	a faster, but also more anomalous, non-Fickian mechanism	884:939	a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin	884:964	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	4	20	theme	anomalous	908:916	arg1	component					978:986	the cement component	967:986	the cement component retaining its crystalline state	967:1018	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	1	21	from	kinetics	150:157	arg1	HAp					196:198	HAp	196:198	HAp	196:198	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	1	21	from	kinetics	150:157	arg1	hydroxyapatite					180:193	hydroxyapatite	180:193	hydroxyapatite (HAp)	180:199	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	8	22	theme	lower	2299:2303	arg1	energy					2313:2318	its lower surface energy	2295:2318	its lower surface energy	2295:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	4	23	theme	Korsmeyer-Peppas	1143:1158	arg1	value					1169:1173	the Korsmeyer-Peppas exponent value	1139:1173	the Korsmeyer-Peppas exponent value of 0.45 ± 0.02	1139:1188	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	8	24	theme	gelled	2361:2366	arg1	state					2379:2383	the gelled, amorphous state	2357:2383	the gelled, amorphous state	2357:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	0	25	theme	phosphate	84:92	arg1	nanopowders					94:104	calcium phosphate nanopowders	76:104	calcium phosphate nanopowders	76:104	Mechanism of formation governs the mechanism of release of antibiotics from calcium phosphate nanopowders and cements in a drug-dependent manner.					
31681475	8	26	from	concentration	2223:2235	arg1	energy					2313:2318	its lower surface energy	2295:2318	its lower surface energy	2295:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	26	from	concentration	2223:2235	arg1	HAp2					2286:2289	HAp2	2286:2289	HAp2	2286:2289	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	5	27	theme	0.45	1342:1345	arg1	±					1347:1347	±	1347:1347	±	1347:1347	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	8	28	theme	release	2577:2583	arg1	mechanisms					2563:2572	mechanisms	2563:2572	mechanisms of release from the two HAp powders with different histories of formation	2563:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	28	theme	release	2577:2583	arg1	rates					2553:2557	the distinct rates	2540:2557	the distinct rates	2540:2557	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	2	29	theme	formation	358:366	arg1	history					347:353	this history	342:353	this history of formation	342:366	Here it is shown that this history of formation affects not only the kinetics, but also the mechanism of release.					
31681475	1	30	theme	crystallization	255:269	arg1	kinetics					243:250	the kinetics	239:250	the kinetics of crystallization of their HAp precursor powders during synthesis	239:317	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	6	31	theme	mechanism	1659:1667	arg1	determinant					1640:1650	an essential determinant	1627:1650	an essential determinant of the mechanism of release	1627:1678	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	6	31	theme	mechanism	1659:1667	arg1	structure					1602:1610	the molecular structure	1588:1610	the molecular structure of the drug	1588:1622	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	5	32	theme	component	1400:1408	arg1	content					1378:1384	the content	1374:1384	the content of the cement component retaining its crystallinity during gelation	1374:1452	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	0	33	theme	formation	13:21	arg1	Mechanism					0:8	Mechanism	0:8	Mechanism of formation	0:21	Mechanism of formation governs the mechanism of release of antibiotics from calcium phosphate nanopowders and cements in a drug-dependent manner.					
31681475	4	34	theme	non-Fickian	919:929	arg1	mechanism					931:939	a faster, but also more anomalous, non-Fickian mechanism	884:939	a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin	884:964	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	4	34	theme	non-Fickian	919:929	arg1	component					978:986	the cement component	967:986	the cement component retaining its crystalline state	967:1018	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	8	35	theme	HAp	2598:2600	arg1	powders					2602:2608	the two HAp powders	2590:2608	the two HAp powders with different histories of formation	2590:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	36	from	surface	2448:2454	arg1	state					2379:2383	the gelled, amorphous state	2357:2383	the gelled, amorphous state	2357:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	7	37	theme	diffusional	2046:2056	arg1	mechanisms					2078:2087	less diffusional and more multimodal mechanisms	2041:2087	less diffusional and more multimodal mechanisms	2041:2087	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	8	38	from	powders	2602:2608	arg1	mechanisms					2563:2572	mechanisms	2563:2572	mechanisms of release from the two HAp powders with different histories of formation	2563:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	38	from	powders	2602:2608	arg1	rates					2553:2557	the distinct rates	2540:2557	the distinct rates	2540:2557	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	38	from	powders	2602:2608	arg1	release					2577:2583	release	2577:2583	release from the two HAp powders with different histories of formation	2577:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	1	39	theme	precursor	284:292	arg1	powders					294:300	their HAp precursor powders	274:300	their HAp precursor powders	274:300	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	8	40	from	content	2484:2490	arg1	state					2379:2383	the gelled, amorphous state	2357:2383	the gelled, amorphous state	2357:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	2	41	theme	release	425:431	arg1	mechanism					412:420	the mechanism	408:420	the mechanism of release	408:431	Here it is shown that this history of formation affects not only the kinetics, but also the mechanism of release.					
31681475	2	41	theme	release	425:431	arg1	kinetics					389:396	the kinetics	385:396	the kinetics	385:396	Here it is shown that this history of formation affects not only the kinetics, but also the mechanism of release.					
31681475	6	42	theme	essential	1630:1638	arg1	determinant					1640:1650	an essential determinant	1627:1650	an essential determinant of the mechanism of release	1627:1678	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	6	42	theme	essential	1630:1638	arg1	structure					1602:1610	the molecular structure	1588:1610	the molecular structure of the drug	1588:1622	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	5	43	theme	case	1284:1287	arg1	HAp2					1289:1292	case HAp2	1284:1292	case HAp2	1284:1292	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	8	44	from	energy	2313:2318	arg1	concentration					2223:2235	a finite concentration	2214:2235	a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy	2214:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	44	from	energy	2313:2318	arg1	potential					2344:2352	lesser drug binding potential	2324:2352	lesser drug binding potential	2324:2352	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	4	45	theme	crystalline	1002:1012	arg1	state					1014:1018	its crystalline state	998:1018	its crystalline state	998:1018	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	4	46	theme	0.45	1178:1181	arg1	±					1183:1183	0.45 ± 0.02	1178:1188	0.45 ± 0.02	1178:1188	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	6	47	theme	passive	1769:1775	arg1	transport					1777:1785	the passive transport	1765:1785	the passive transport	1765:1785	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	8	48	theme	formation	2638:2646	arg1	histories					2625:2633	different histories	2615:2633	different histories of formation	2615:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	49	theme	particle	2439:2446	arg1	content					2484:2490	higher structural water content	2460:2490	higher structural water content	2460:2490	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	49	theme	particle	2439:2446	arg1	surface					2448:2454	its possibly less stable and more diffusive particle surface	2395:2454	its possibly less stable and more diffusive particle surface	2395:2454	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	49	theme	particle	2439:2446	arg1	retention					2201:2209	the retention	2197:2209	the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state	2197:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	49	theme	particle	2439:2446	arg1	reasons					2521:2527	potential reasons	2511:2527	potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation	2511:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	3	50	dep	Cements	434:440	arg1	composed					442:449	composed	442:449	Cements composed of two HAp powders precipitated under different conditions	434:508	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	4	51	theme	cement	971:976	arg1	component					978:986	the cement component	967:986	the cement component retaining its crystalline state	967:1018	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	4	51	theme	cement	971:976	arg1	mechanism					931:939	a faster, but also more anomalous, non-Fickian mechanism	884:939	a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin	884:964	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	4	52	theme	cement	800:805	arg1	component					807:815	the cement component	796:815	the cement component converting to the amorphous phase during gelation (HAp2)	796:872	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	8	53	theme	structural	2467:2476	arg1	content					2484:2490	higher structural water content	2460:2490	higher structural water content	2460:2490	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	53	theme	structural	2467:2476	arg1	surface					2448:2454	its possibly less stable and more diffusive particle surface	2395:2454	its possibly less stable and more diffusive particle surface	2395:2454	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	53	theme	structural	2467:2476	arg1	retention					2201:2209	the retention	2197:2209	the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state	2197:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	53	theme	structural	2467:2476	arg1	reasons					2521:2527	potential reasons	2511:2527	potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation	2511:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	54	from	potential	2344:2352	arg1	energy					2313:2318	its lower surface energy	2295:2318	its lower surface energy	2295:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	54	from	potential	2344:2352	arg1	HAp2					2286:2289	HAp2	2286:2289	HAp2	2286:2289	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	55	with	powders	2602:2608	arg1	histories					2625:2633	different histories	2615:2633	different histories of formation	2615:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	56	theme	stable	2413:2418	arg1	content					2484:2490	higher structural water content	2460:2490	higher structural water content	2460:2490	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	56	theme	stable	2413:2418	arg1	surface					2448:2454	its possibly less stable and more diffusive particle surface	2395:2454	its possibly less stable and more diffusive particle surface	2395:2454	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	56	theme	stable	2413:2418	arg1	retention					2201:2209	the retention	2197:2209	the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state	2197:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	56	theme	stable	2413:2418	arg1	reasons					2521:2527	potential reasons	2511:2527	potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation	2511:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	6	57	theme	release	1672:1678	arg1	mechanism					1659:1667	the mechanism	1655:1667	the mechanism of release	1655:1678	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	5	58	theme	=	1340:1340	arg1	n					1338:1338	n	1338:1338	n = 0.45 ± 0.02	1338:1352	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	1	59	theme	drug	162:165	arg1	release					167:173	drug release	162:173	drug release from hydroxyapatite (HAp)	162:199	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	8	60	theme	finite	2216:2221	arg1	concentration					2223:2235	a finite concentration	2214:2235	a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy	2214:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	6	61	theme	drugs	1750:1754	arg1	release					1739:1745	a universally tunable release	1717:1745	a universally tunable release of drugs based on the passive transport	1717:1785	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	8	62	from	rates	2553:2557	arg1	powders					2602:2608	the two HAp powders	2590:2608	the two HAp powders with different histories of formation	2590:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	5	63	theme	release	1518:1524	arg1	mechanism					1505:1513	the mechanism	1501:1513	the mechanism of release	1501:1524	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	5	64	theme	Fickian	1315:1321	arg1	diffusion					1323:1331	the ideal, Fickian diffusion	1304:1331	the ideal, Fickian diffusion	1304:1331	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	8	65	theme	pores	2250:2254	arg1	concentration					2223:2235	a finite concentration	2214:2235	a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy	2214:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	65	theme	pores	2250:2254	arg1	potential					2344:2352	lesser drug binding potential	2324:2352	lesser drug binding potential	2324:2352	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	3	66	theme	drug	667:670	arg1	kinetics					680:687	their drug release kinetics	661:687	their drug release kinetics	661:687	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	8	67	theme	different	2615:2623	arg1	histories					2625:2633	different histories	2615:2633	different histories of formation	2615:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	68	from	retention	2201:2209	arg1	state					2379:2383	the gelled, amorphous state	2357:2383	the gelled, amorphous state	2357:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	69	theme	precursor	2273:2281	arg1	concentration					2223:2235	a finite concentration	2214:2235	a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy	2214:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	69	theme	precursor	2273:2281	arg1	potential					2344:2352	lesser drug binding potential	2324:2352	lesser drug binding potential	2324:2352	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	4	70	theme	diffusion	1107:1115	arg1	case					1117:1120	Fickian diffusion case	1099:1120	Fickian diffusion case	1099:1120	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	7	71	theme	additional	1953:1962	arg1	effects					1964:1970	additional effects	1953:1970	additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms	1953:2087	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	8	72	theme	drug	2331:2334	arg1	potential					2344:2352	lesser drug binding potential	2324:2352	lesser drug binding potential	2324:2352	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	73	theme	potential	2511:2519	arg1	content					2484:2490	higher structural water content	2460:2490	higher structural water content	2460:2490	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	73	theme	potential	2511:2519	arg1	surface					2448:2454	its possibly less stable and more diffusive particle surface	2395:2454	its possibly less stable and more diffusive particle surface	2395:2454	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	73	theme	potential	2511:2519	arg1	retention					2201:2209	the retention	2197:2209	the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state	2197:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	73	theme	potential	2511:2519	arg1	reasons					2521:2527	potential reasons	2511:2527	potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation	2511:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	5	74	dep	=	1340:1340	arg1	0.02					1349:1352	0.02	1349:1352	0.02	1349:1352	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	0	75	from	nanopowders	94:104	arg1	mechanism					35:43	the mechanism	31:43	the mechanism of release of antibiotics from calcium phosphate nanopowders	31:104	Mechanism of formation governs the mechanism of release of antibiotics from calcium phosphate nanopowders and cements in a drug-dependent manner.					
31681475	0	75	from	nanopowders	94:104	arg1	release					48:54	release	48:54	release of antibiotics from calcium phosphate nanopowders	48:104	Mechanism of formation governs the mechanism of release of antibiotics from calcium phosphate nanopowders and cements in a drug-dependent manner.					
31681475	7	76	theme	erosion	1942:1948	arg1	inclusion					1916:1924	the inclusion	1912:1924	the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms	1912:2087	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	8	77	theme	potential	2344:2352	arg1	content					2484:2490	higher structural water content	2460:2490	higher structural water content	2460:2490	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	77	theme	potential	2344:2352	arg1	surface					2448:2454	its possibly less stable and more diffusive particle surface	2395:2454	its possibly less stable and more diffusive particle surface	2395:2454	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	77	theme	potential	2344:2352	arg1	retention					2201:2209	the retention	2197:2209	the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state	2197:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	77	theme	potential	2344:2352	arg1	reasons					2521:2527	potential reasons	2511:2527	potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation	2511:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	0	78	theme	antibiotics	59:69	arg1	release					48:54	release	48:54	release of antibiotics from calcium phosphate nanopowders	48:104	Mechanism of formation governs the mechanism of release of antibiotics from calcium phosphate nanopowders and cements in a drug-dependent manner.					
31681475	1	79	from	hydroxyapatite	180:193	arg1	kinetics					150:157	The kinetics	146:157	The kinetics of drug release from hydroxyapatite (HAp)	146:199	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	1	79	from	hydroxyapatite	180:193	arg1	release					167:173	drug release	162:173	drug release from hydroxyapatite (HAp)	162:199	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	6	80	theme	drug	1619:1622	arg1	determinant					1640:1650	an essential determinant	1627:1650	an essential determinant of the mechanism of release	1627:1678	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	6	80	theme	drug	1619:1622	arg1	structure					1602:1610	the molecular structure	1588:1610	the molecular structure of the drug	1588:1622	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	5	81	dep	anomalous	1534:1542	arg1	non-Fickian					1545:1555	non-Fickian	1545:1555	non-Fickian	1545:1555	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	7	82	theme	swelling	1929:1936	arg1	inclusion					1916:1924	the inclusion	1912:1924	the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms	1912:2087	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	0	83	theme	calcium	76:82	arg1	nanopowders					94:104	calcium phosphate nanopowders	76:104	calcium phosphate nanopowders	76:104	Mechanism of formation governs the mechanism of release of antibiotics from calcium phosphate nanopowders and cements in a drug-dependent manner.					
31681475	3	84	with	conjunction	729:739	arg1	analyses					780:787	compositional and microstructural analyses	746:787	compositional and microstructural analyses	746:787	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	8	85	theme	surface	2305:2311	arg1	energy					2313:2318	its lower surface energy	2295:2318	its lower surface energy	2295:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	4	86	theme	exponent	1160:1167	arg1	value					1169:1173	the Korsmeyer-Peppas exponent value	1139:1173	the Korsmeyer-Peppas exponent value of 0.45 ± 0.02	1139:1188	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	3	87	theme	microstructural	764:778	arg1	analyses					780:787	compositional and microstructural analyses	746:787	compositional and microstructural analyses	746:787	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	6	88	theme	molecular	1592:1600	arg1	determinant					1640:1650	an essential determinant	1627:1650	an essential determinant of the mechanism of release	1627:1678	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	6	88	theme	molecular	1592:1600	arg1	structure					1602:1610	the molecular structure	1588:1610	the molecular structure of the drug	1588:1622	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	8	89	theme	distinct	2544:2551	arg1	rates					2553:2557	the distinct rates	2540:2557	the distinct rates	2540:2557	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	3	90	theme	compositional	746:758	arg1	analyses					780:787	compositional and microstructural analyses	746:787	compositional and microstructural analyses	746:787	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	8	91	from	precursor	2273:2281	arg1	energy					2313:2318	its lower surface energy	2295:2318	its lower surface energy	2295:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	91	from	precursor	2273:2281	arg1	HAp2					2286:2289	HAp2	2286:2289	HAp2	2286:2289	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	4	92	theme	faster	886:891	arg1	mechanism					931:939	a faster, but also more anomalous, non-Fickian mechanism	884:939	a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin	884:964	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	4	92	theme	faster	886:891	arg1	component					978:986	the cement component	967:986	the cement component retaining its crystalline state	967:1018	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	0	93	from	mechanism	35:43	arg1	nanopowders					94:104	calcium phosphate nanopowders	76:104	calcium phosphate nanopowders	76:104	Mechanism of formation governs the mechanism of release of antibiotics from calcium phosphate nanopowders and cements in a drug-dependent manner.					
31681475	4	94	theme	release	944:950	arg1	mechanism					931:939	a faster, but also more anomalous, non-Fickian mechanism	884:939	a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin	884:964	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	4	94	theme	release	944:950	arg1	component					978:986	the cement component	967:986	the cement component retaining its crystalline state	967:1018	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	4	95	theme	vancomycin	955:964	arg1	release					944:950	release	944:950	release of vancomycin	944:964	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	7	96	theme	multimodal	2067:2076	arg1	mechanisms					2078:2087	less diffusional and more multimodal mechanisms	2041:2087	less diffusional and more multimodal mechanisms	2041:2087	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	5	97	theme	anomalous	1534:1542	arg1	types					1557:1561	more anomalous, non-Fickian types	1529:1561	more anomalous, non-Fickian types	1529:1561	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	1	98	theme	HAp	280:282	arg1	powders					294:300	their HAp precursor powders	274:300	their HAp precursor powders	274:300	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	8	99	from	HAp2	2286:2289	arg1	concentration					2223:2235	a finite concentration	2214:2235	a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy	2214:2318	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	99	from	HAp2	2286:2289	arg1	potential					2344:2352	lesser drug binding potential	2324:2352	lesser drug binding potential	2324:2352	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	100	theme	microstructural	2109:2123	arg1	effects					2143:2149	the microstructural and compositional effects	2105:2149	the microstructural and compositional effects governing the release mechanism and kinetics	2105:2194	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	3	101	theme	crystalline	581:591	arg1	state					593:597	the crystalline state	577:597	the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses	577:787	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	0	102	theme	release	48:54	arg1	mechanism					35:43	the mechanism	31:43	the mechanism of release of antibiotics from calcium phosphate nanopowders	31:104	Mechanism of formation governs the mechanism of release of antibiotics from calcium phosphate nanopowders and cements in a drug-dependent manner.					
31681475	1	103	theme	powders	294:300	arg1	crystallization					255:269	crystallization	255:269	crystallization of their HAp precursor powders during synthesis	255:317	The kinetics of drug release from hydroxyapatite (HAp) cements could be tuned by controlling the kinetics of crystallization of their HAp precursor powders during synthesis.					
31681475	8	104	theme	compositional	2129:2141	arg1	effects					2143:2149	the microstructural and compositional effects	2105:2149	the microstructural and compositional effects governing the release mechanism and kinetics	2105:2194	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	105	dep	gelled	2361:2366	arg1	amorphous					2369:2377	amorphous	2369:2377	amorphous	2369:2377	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	4	106	theme	±	1183:1183	arg1	value					1169:1173	the Korsmeyer-Peppas exponent value	1139:1173	the Korsmeyer-Peppas exponent value of 0.45 ± 0.02	1139:1188	While the cement component converting to the amorphous phase during gelation (HAp2) exhibited a faster, but also more anomalous, non-Fickian mechanism of release of vancomycin, the cement component retaining its crystalline state all throughout gelation, setting and hardening (HAp1) stabilized at the ideal, Fickian diffusion case corresponding to the Korsmeyer-Peppas exponent value of 0.45 ± 0.02.					
31681475	8	107	theme	diffusive	2429:2437	arg1	content					2484:2490	higher structural water content	2460:2490	higher structural water content	2460:2490	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	107	theme	diffusive	2429:2437	arg1	surface					2448:2454	its possibly less stable and more diffusive particle surface	2395:2454	its possibly less stable and more diffusive particle surface	2395:2454	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	107	theme	diffusive	2429:2437	arg1	retention					2201:2209	the retention	2197:2209	the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state	2197:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	107	theme	diffusive	2429:2437	arg1	reasons					2521:2527	potential reasons	2511:2527	potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation	2511:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	3	108	theme	HAp	458:460	arg1	powders					462:468	two HAp powders	454:468	two HAp powders precipitated under different conditions	454:508	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31681475	6	109	theme	carrier	1705:1711	arg1	impossible					1797:1806	impossible	1797:1806	impossible	1797:1806	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	6	109	theme	carrier	1705:1711	arg1	design					1693:1698	the design	1689:1698	the design of a carrier for a universally tunable release of drugs based on the passive transport	1689:1785	This has indicated that the molecular structure of the drug is an essential determinant of the mechanism of release and that the design of a carrier for a universally tunable release of drugs based on the passive transport is likely impossible.					
31681475	5	110	theme	cement	1393:1398	arg1	component					1400:1408	the cement component	1389:1408	the cement component retaining its crystallinity during gelation	1389:1452	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	8	111	theme	release	2165:2171	arg1	mechanism					2173:2181	the release mechanism	2161:2181	the release mechanism	2161:2181	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	5	112	from	n	1338:1338	arg1	content					1378:1384	the content	1374:1384	the content of the cement component retaining its crystallinity during gelation	1374:1452	This effect got reversed for the other antibiotic studied as a drug, ciprofloxacin, in which case HAp2 exhibited the ideal, Fickian diffusion with n = 0.45 ± 0.02 and the increase in the content of the cement component retaining its crystallinity during gelation, setting and hardening (HAp1) steadily shifted the mechanism of release to more anomalous, non-Fickian types.					
31681475	8	113	theme	lesser	2324:2329	arg1	potential					2344:2352	lesser drug binding potential	2324:2352	lesser drug binding potential	2324:2352	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	7	114	theme	shifted	2014:2020	arg1	release					2026:2032	the release	2022:2032	shifted the release	2014:2032	Preliminary assays involving the addition of chitosan or gelatin as polymeric components to HAp led to the inclusion of swelling and erosion as additional effects by which the drug escapes the carrier and shifted the release toward less diffusional and more multimodal mechanisms.					
31681475	8	115	theme	higher	2460:2465	arg1	content					2484:2490	higher structural water content	2460:2490	higher structural water content	2460:2490	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	115	theme	higher	2460:2465	arg1	surface					2448:2454	its possibly less stable and more diffusive particle surface	2395:2454	its possibly less stable and more diffusive particle surface	2395:2454	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	115	theme	higher	2460:2465	arg1	retention					2201:2209	the retention	2197:2209	the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state	2197:2383	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	8	115	theme	higher	2460:2465	arg1	reasons					2521:2527	potential reasons	2511:2527	potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation	2511:2646	With regard to the microstructural and compositional effects governing the release mechanism and kinetics, the retention of a finite concentration of slit-like pores of the amorphous precursor in HAp2 and its lower surface energy and lesser drug binding potential in the gelled, amorphous state, but also its possibly less stable and more diffusive particle surface and higher structural water content were elaborated as potential reasons explaining the distinct rates and mechanisms of release from the two HAp powders with different histories of formation.					
31681475	3	116	theme	different	489:497	arg1	conditions					499:508	different conditions	489:508	different conditions	489:508	Cements composed of two HAp powders precipitated under different conditions, one (HAp2) taking twice longer to transform from the amorphous to the crystalline state than the other (HAp1), were mixed at different ratios to tune their drug release kinetics and tested for the release mechanism in conjunction with compositional and microstructural analyses.					
31228462	4	0	theme	eye	548:550	arg1	cornea					529:534	the central cornea	517:534	the central cornea of the left eye	517:550	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	6	1	theme	time	732:735	arg1	point					737:741	each time point	727:741	each time point	727:741	At each time point, the clinical conditions of the burned and control corneas were observed.					
31228462	11	2	theme	different	1364:1372	arg1	types					1374:1378	different types	1364:1378	different types of cells and collagen type III fibers	1364:1416	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	2	3	theme	healing	264:270	arg1	process					272:278	the corneal wound healing process	246:278	the corneal wound healing process	246:278	We monitored the corneal wound healing process after alkali burns in rabbits.					
31228462	14	4	theme	light	1675:1679	arg1	transmittance					1681:1693	increased light transmittance	1665:1693	increased light transmittance	1665:1693	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	4	5	theme	0.5 N	614:618	arg1	NaOH					620:623	0.5 N NaOH	614:623	0.5 N NaOH	614:623	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	2	6	theme	corneal	250:256	arg1	healing					264:270	the corneal wound healing	246:270	the corneal wound healing process	246:278	We monitored the corneal wound healing process after alkali burns in rabbits.					
31228462	1	7	theme	precise	155:161	arg1	organization					163:174	the precise organization	151:174	the precise organization of collagen fibrils	151:194	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	11	8	theme	alkali	1291:1296	arg1	burn					1298:1301	alkali burn	1291:1301	alkali burn	1291:1301	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	4	9	theme	filter	591:596	arg1	paper					598:602	an 8-mm diameter filter paper	574:602	an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s	574:632	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	14	10	theme	increased	1665:1673	arg1	transmittance					1681:1693	increased light transmittance	1665:1693	increased light transmittance	1665:1693	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	13	11	theme	mRNA	1538:1541	arg1	levels					1554:1559	Collagen and proteoglycan mRNA expression levels	1512:1559	Collagen and proteoglycan mRNA expression levels	1512:1559	Collagen and proteoglycan mRNA expression levels were up-regulated.					
31228462	1	12	theme	fibrils	188:194	arg1	organization					163:174	the precise organization	151:174	the precise organization of collagen fibrils	151:194	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	1	12	theme	fibrils	188:194	arg1	fibrils					188:194	collagen fibrils	179:194	collagen fibrils	179:194	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	1	12	theme	fibrils	188:194	arg1	proteoglycans					218:230	proteoglycans	218:230	proteoglycans	218:230	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	1	12	theme	fibrils	188:194	arg1	variety					207:213	a wide variety	200:213	a wide variety of proteoglycans	200:230	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	6	13	located	observed	807:814	arg1	point					737:741	each time point	727:741	each time point	727:741	At each time point, the clinical conditions of the burned and control corneas were observed.					
31228462	6	13	located	observed	807:814	arg2	conditions					757:766	the clinical conditions	744:766	the clinical conditions of the burned and control corneas	744:800	At each time point, the clinical conditions of the burned and control corneas were observed.					
31228462	16	14	theme	ECM	2130:2132	arg1	composition					2134:2144	ECM composition	2130:2144	ECM composition	2130:2144	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	16	14	theme	ECM	2130:2132	arg1	changes					2045:2051	changes	2045:2051	changes in corneal transmittance	2045:2076	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	14	15	theme	opacity	1656:1662	arg1	levels					1766:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	15	16	theme	area	1825:1828	arg1	similar					1849:1855	similar	1849:1855	similar	1849:1855	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	15	16	theme	area	1825:1828	arg1	morphology					1834:1843	the wound area ECM morphology	1815:1843	the wound area ECM morphology	1815:1843	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	7	17	theme	electronic	964:973	arg1	microscopy					975:984	transmission electronic microscopy	951:984	transmission electronic microscopy	951:984	The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy.					
31228462	14	18	theme	reduced	1640:1646	arg1	levels					1766:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	7	19	theme	Picrosirius-red	892:906	arg1	staining					908:915	Picrosirius-red staining	892:915	Picrosirius-red staining	892:915	The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy.					
31228462	9	20	theme	Myofibroblasts	1034:1047	arg1	presence					1049:1056	Myofibroblasts presence	1034:1056	Myofibroblasts presence	1034:1056	Myofibroblasts presence was analyzed by immunohistochemistry.					
31228462	0	21	theme	wound	59:63	arg1	healing					65:71	corneal wound healing	51:71	corneal wound healing	51:71	Dynamic changes of the extracellular matrix during corneal wound healing.					
31228462	12	22	theme	transmittance	1497:1509	arg1	loss					1483:1486	attendant loss	1473:1486	attendant loss of light transmittance	1473:1509	Corneal opacities were present with attendant loss of light transmittance.					
31228462	14	23	theme	wound	1600:1604	arg1	healing					1606:1612	wound healing	1600:1612	wound healing progress	1600:1621	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	14	24	theme	control	1781:1787	arg1	levels					1789:1794	control levels	1781:1794	control levels	1781:1794	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	12	25	theme	Corneal	1437:1443	arg1	opacities					1445:1453	Corneal opacities	1437:1453	Corneal opacities	1437:1453	Corneal opacities were present with attendant loss of light transmittance.					
31228462	17	26	theme	wound	2202:2206	arg1	resolution					2208:2217	wound resolution	2202:2217	wound resolution	2202:2217	Documenting wound resolution during the six-month period provided reliable information that can be used to test new therapies.					
31228462	16	27	theme	corneal	2056:2062	arg1	transmittance					2064:2076	corneal transmittance	2056:2076	corneal transmittance	2056:2076	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	10	28	theme	alpha-smooth	1180:1191	arg1	actin					1200:1204	alpha-smooth muscle actin	1180:1204	alpha-smooth muscle actin	1180:1204	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	28	theme	alpha-smooth	1180:1191	arg1	types					1131:1135	collagen types I and III	1122:1145	collagen types I and III	1122:1145	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	29	theme	chain	1259:1263	arg1	reaction					1265:1272	quantitative real-time polymerase chain reaction	1225:1272	quantitative real-time polymerase chain reaction	1225:1272	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	12	30	theme	attendant	1473:1481	arg1	loss					1483:1486	attendant loss	1473:1486	attendant loss of light transmittance	1473:1509	Corneal opacities were present with attendant loss of light transmittance.					
31228462	15	31	theme	transmittance	1874:1886	arg1	values					1888:1893	transmittance values	1874:1893	transmittance values	1874:1893	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	10	32	theme	quantitative	1225:1236	arg1	reaction					1265:1272	quantitative real-time polymerase chain reaction	1225:1272	quantitative real-time polymerase chain reaction	1225:1272	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	7	33	theme	fibers	845:850	arg1	arrangement					821:831	The arrangement	817:831	The arrangement of collagen fibers in the corneal stroma	817:872	The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy.					
31228462	17	34	theme	six-month	2230:2238	arg1	period					2240:2245	the six-month period	2226:2245	the six-month period	2226:2245	Documenting wound resolution during the six-month period provided reliable information that can be used to test new therapies.					
31228462	0	35	theme	Dynamic	0:6	arg1	changes					8:14	Dynamic changes	0:14	Dynamic changes of the extracellular matrix during corneal wound healing.	0:72	Dynamic changes of the extracellular matrix during corneal wound healing.					
31228462	8	36	theme	Corneal	987:993	arg1	transmittance					1001:1013	Corneal light transmittance	987:1013	Corneal light transmittance	987:1013	Corneal light transmittance was also measured.					
31228462	4	37	theme	central	521:527	arg1	cornea					529:534	the central cornea	517:534	the central cornea of the left eye	517:550	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	11	38	theme	fibers	1411:1416	arg1	types					1374:1378	different types	1364:1378	different types of cells and collagen type III fibers	1364:1416	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	3	39	theme	transparency	432:443	arg1	recovery					412:419	the recovery	408:419	the recovery of optical transparency	408:443	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	3	39	theme	transparency	432:443	arg1	location					327:334	location	327:334	location	327:334	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	3	39	theme	transparency	432:443	arg1	expression					340:349	expression	340:349	expression	340:349	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	3	39	theme	transparency	432:443	arg1	impact					396:401	the clinical impact	383:401	the clinical impact	383:401	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	16	40	from	changes	2045:2051	arg1	transmittance					2064:2076	corneal transmittance	2056:2076	corneal transmittance	2056:2076	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	6	41	theme	burned	775:780	arg1	conditions					757:766	the clinical conditions	744:766	the clinical conditions of the burned and control corneas	744:800	At each time point, the clinical conditions of the burned and control corneas were observed.					
31228462	10	42	theme	polymerase	1248:1257	arg1	reaction					1265:1272	quantitative real-time polymerase chain reaction	1225:1272	quantitative real-time polymerase chain reaction	1225:1272	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	3	43	theme	clinical	387:394	arg1	impact					396:401	the clinical impact	383:401	the clinical impact	383:401	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	15	44	theme	stromal	1948:1954	arg1	architecture					1956:1967	the stromal architecture	1944:1967	the stromal architecture	1944:1967	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	11	45	theme	type	1402:1405	arg1	fibers					1411:1416	collagen type III fibers	1393:1416	collagen type III fibers	1393:1416	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	0	46	theme	extracellular	23:35	arg1	matrix					37:42	the extracellular matrix	19:42	the extracellular matrix	19:42	Dynamic changes of the extracellular matrix during corneal wound healing.					
31228462	4	47	theme	topical	497:503	arg1	anesthesia					505:514	both general and ocular topical anesthesia	473:514	both general and ocular topical anesthesia	473:514	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	16	48	theme	wound	2014:2018	arg1	healing					2020:2026	the stromal wound healing	2002:2026	the stromal wound healing process	2002:2034	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	10	49	theme	expression	1101:1110	arg1	levels					1112:1117	mRNA expression levels	1096:1117	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin	1096:1204	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	50	dep	types	1131:1135	arg1	I					1137:1137	I	1137:1137	I	1137:1137	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	50	dep	types	1131:1135	arg1	types					1131:1135	collagen types I and III	1122:1145	collagen types I and III	1122:1145	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	50	dep	types	1131:1135	arg1	III					1143:1145	III	1143:1145	III	1143:1145	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	15	51	theme	incomplete	1918:1927	arg1	restoration					1929:1939	incomplete restoration	1918:1939	incomplete restoration of the stromal architecture	1918:1967	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	1	52	theme	wide	202:205	arg1	fibrils					188:194	collagen fibrils	179:194	collagen fibrils	179:194	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	1	52	theme	wide	202:205	arg1	proteoglycans					218:230	proteoglycans	218:230	proteoglycans	218:230	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	1	52	theme	wide	202:205	arg1	variety					207:213	a wide variety	200:213	a wide variety of proteoglycans	200:230	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	10	53	theme	types	1131:1135	arg1	levels					1112:1117	mRNA expression levels	1096:1117	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin	1096:1204	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	14	54	theme	reorganization	1696:1709	arg1	levels					1766:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	11	55	dep	month	1279:1283	arg1	burn					1298:1301	alkali burn	1291:1301	alkali burn	1291:1301	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	4	56	theme	left	543:546	arg1	eye					548:550	the left eye	539:550	the left eye	539:550	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	13	57	theme	expression	1543:1552	arg1	levels					1554:1559	Collagen and proteoglycan mRNA expression levels	1512:1559	Collagen and proteoglycan mRNA expression levels	1512:1559	Collagen and proteoglycan mRNA expression levels were up-regulated.					
31228462	14	58	theme	transmittance	1681:1693	arg1	levels					1766:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	16	59	theme	alkali	2174:2179	arg1	burning					2181:2187	alkali burning	2174:2187	alkali burning	2174:2187	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	2	60	theme	wound	258:262	arg1	healing					264:270	the corneal wound healing	246:270	the corneal wound healing process	246:278	We monitored the corneal wound healing process after alkali burns in rabbits.					
31228462	6	61	theme	control	786:792	arg1	conditions					757:766	the clinical conditions	744:766	the clinical conditions of the burned and control corneas	744:800	At each time point, the clinical conditions of the burned and control corneas were observed.					
31228462	11	62	contain	containing	1353:1362	arg1	lacunae					1345:1351	lacunae	1345:1351	lacunae containing different types of cells and collagen type III fibers in the wound area	1345:1434	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	11	62	contain	containing	1353:1362	arg2	types					1374:1378	different types	1364:1378	different types of cells and collagen type III fibers	1364:1416	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	14	63	theme	collagen	1739:1746	arg1	type					1748:1751	only collagen type I	1734:1753	only collagen type I	1734:1753	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	16	64	theme	myofibroblasts	2102:2115	arg1	presence					2117:2124	myofibroblasts presence	2102:2124	myofibroblasts presence	2102:2124	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	16	64	theme	myofibroblasts	2102:2115	arg1	changes					2045:2051	changes	2045:2051	changes in corneal transmittance	2045:2076	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	13	65	theme	Collagen	1512:1519	arg1	levels					1554:1559	Collagen and proteoglycan mRNA expression levels	1512:1559	Collagen and proteoglycan mRNA expression levels	1512:1559	Collagen and proteoglycan mRNA expression levels were up-regulated.					
31228462	16	66	theme	stromal	2006:2012	arg1	healing					2020:2026	the stromal wound healing	2002:2026	the stromal wound healing process	2002:2034	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	6	67	theme	clinical	748:755	arg1	conditions					757:766	the clinical conditions	744:766	the clinical conditions of the burned and control corneas	744:800	At each time point, the clinical conditions of the burned and control corneas were observed.					
31228462	2	68	theme	alkali	286:291	arg1	burns					293:297	alkali burns	286:297	alkali burns in rabbits	286:308	We monitored the corneal wound healing process after alkali burns in rabbits.					
31228462	1	69	theme	collagen	179:186	arg1	fibrils					188:194	collagen fibrils	179:194	collagen fibrils	179:194	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	4	70	theme	diameter	582:589	arg1	paper					598:602	an 8-mm diameter filter paper	574:602	an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s	574:632	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	15	71	theme	ECM	1830:1832	arg1	similar					1849:1855	similar	1849:1855	similar	1849:1855	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	15	71	theme	ECM	1830:1832	arg1	morphology					1834:1843	the wound area ECM morphology	1815:1843	the wound area ECM morphology	1815:1843	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	17	72	theme	reliable	2256:2263	arg1	information					2265:2275	reliable information	2256:2275	reliable information that can be used to test new therapies	2256:2314	Documenting wound resolution during the six-month period provided reliable information that can be used to test new therapies.					
31228462	14	73	theme	only	1734:1737	arg1	type					1748:1751	only collagen type I	1734:1753	only collagen type I	1734:1753	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	15	74	theme	wound	1819:1823	arg1	similar					1849:1855	similar	1849:1855	similar	1849:1855	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	15	74	theme	wound	1819:1823	arg1	morphology					1834:1843	the wound area ECM morphology	1815:1843	the wound area ECM morphology	1815:1843	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	7	75	theme	transmission	951:962	arg1	microscopy					975:984	transmission electronic microscopy	951:984	transmission electronic microscopy	951:984	The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy.					
31228462	14	76	theme	corneal	1648:1654	arg1	opacity					1656:1662	corneal opacity	1648:1662	corneal opacity	1648:1662	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	7	77	theme	silver	927:932	arg1	impregnation					934:945	Gomori's silver impregnation	918:945	Gomori's silver impregnation	918:945	The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy.					
31228462	11	78	theme	wound	1425:1429	arg1	area					1431:1434	the wound area	1421:1434	the wound area	1421:1434	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	16	79	theme	process	2028:2034	arg1	study					1993:1997	This multidisciplinary study	1970:1997	This multidisciplinary study of the stromal wound healing process	1970:2034	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	13	80	theme	proteoglycan	1525:1536	arg1	levels					1554:1559	Collagen and proteoglycan mRNA expression levels	1512:1559	Collagen and proteoglycan mRNA expression levels	1512:1559	Collagen and proteoglycan mRNA expression levels were up-regulated.					
31228462	12	81	theme	light	1491:1495	arg1	transmittance					1497:1509	light transmittance	1491:1509	light transmittance	1491:1509	Corneal opacities were present with attendant loss of light transmittance.					
31228462	12	82	with	present	1460:1466	arg1	loss					1483:1486	attendant loss	1473:1486	attendant loss of light transmittance	1473:1509	Corneal opacities were present with attendant loss of light transmittance.					
31228462	4	83	theme	general	478:484	arg1	anesthesia					505:514	both general and ocular topical anesthesia	473:514	both general and ocular topical anesthesia	473:514	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	14	84	theme	healing	1606:1612	arg1	progress					1614:1621	wound healing progress	1600:1621	wound healing progress	1600:1621	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	17	85	theme	new	2302:2304	arg1	therapies					2306:2314	new therapies	2302:2314	new therapies	2302:2314	Documenting wound resolution during the six-month period provided reliable information that can be used to test new therapies.					
31228462	4	86	theme	ocular	490:495	arg1	anesthesia					505:514	both general and ocular topical anesthesia	473:514	both general and ocular topical anesthesia	473:514	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	10	87	theme	muscle	1193:1198	arg1	actin					1200:1204	alpha-smooth muscle actin	1180:1204	alpha-smooth muscle actin	1180:1204	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	87	theme	muscle	1193:1198	arg1	types					1131:1135	collagen types I and III	1122:1145	collagen types I and III	1122:1145	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	5	88	theme	surgical	667:674	arg1	microscope					676:685	a surgical microscope	665:685	a surgical microscope	665:685	The eyes were evaluated under a surgical microscope at 1, 3, and 6 months after burning.					
31228462	2	89	from	burns	293:297	arg1	rabbits					302:308	rabbits	302:308	rabbits	302:308	We monitored the corneal wound healing process after alkali burns in rabbits.					
31228462	10	90	theme	real-time	1238:1246	arg1	reaction					1265:1272	quantitative real-time polymerase chain reaction	1225:1272	quantitative real-time polymerase chain reaction	1225:1272	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	11	91	theme	cells	1383:1387	arg1	types					1374:1378	different types	1364:1378	different types of cells and collagen type III fibers	1364:1416	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	7	92	theme	corneal	859:865	arg1	stroma					867:872	the corneal stroma	855:872	the corneal stroma	855:872	The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy.					
31228462	8	93	theme	light	995:999	arg1	transmittance					1001:1013	Corneal light transmittance	987:1013	Corneal light transmittance	987:1013	Corneal light transmittance was also measured.					
31228462	1	94	theme	extracellular	78:90	arg1	matrix					92:97	The extracellular matrix	74:97	The extracellular matrix (ECM)	74:103	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	1	94	theme	extracellular	78:90	arg1	ECM					100:102	ECM	100:102	ECM	100:102	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	3	95	theme	proteoglycans	368:380	arg1	recovery					412:419	the recovery	408:419	the recovery of optical transparency	408:443	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	3	95	theme	proteoglycans	368:380	arg1	location					327:334	location	327:334	location	327:334	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	3	95	theme	proteoglycans	368:380	arg1	expression					340:349	expression	340:349	expression	340:349	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	3	95	theme	proteoglycans	368:380	arg1	impact					396:401	the clinical impact	383:401	the clinical impact	383:401	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	16	96	theme	collagen	2079:2086	arg1	changes					2045:2051	changes	2045:2051	changes in corneal transmittance	2045:2076	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	16	96	theme	collagen	2079:2086	arg1	organization					2088:2099	collagen organization	2079:2099	collagen organization	2079:2099	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	7	97	theme	collagen	836:843	arg1	fibers					845:850	collagen fibers	836:850	collagen fibers	836:850	The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy.					
31228462	3	98	theme	collagens	354:362	arg1	recovery					412:419	the recovery	408:419	the recovery of optical transparency	408:443	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	3	98	theme	collagens	354:362	arg1	location					327:334	location	327:334	location	327:334	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	3	98	theme	collagens	354:362	arg1	expression					340:349	expression	340:349	expression	340:349	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	3	98	theme	collagens	354:362	arg1	impact					396:401	the clinical impact	383:401	the clinical impact	383:401	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	16	99	theme	multidisciplinary	1975:1991	arg1	study					1993:1997	This multidisciplinary study	1970:1997	This multidisciplinary study of the stromal wound healing process	1970:2034	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	7	100	from	arrangement	821:831	arg1	stroma					867:872	the corneal stroma	855:872	the corneal stroma	855:872	The arrangement of collagen fibers in the corneal stroma was visualized by Picrosirius-red staining, Gomori's silver impregnation and transmission electronic microscopy.					
31228462	11	101	theme	collagen	1393:1400	arg1	type					1402:1405	collagen type III	1393:1409	collagen type III fibers	1393:1416	One month after alkali burn, the ECM was disorganized and filled with lacunae containing different types of cells and collagen type III fibers in the wound area.					
31228462	0	102	theme	matrix	37:42	arg1	changes					8:14	Dynamic changes	0:14	Dynamic changes of the extracellular matrix during corneal wound healing.	0:72	Dynamic changes of the extracellular matrix during corneal wound healing.					
31228462	14	103	theme	expression	1755:1764	arg1	levels					1766:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	15	104	theme	architecture	1956:1967	arg1	restoration					1929:1939	incomplete restoration	1918:1939	incomplete restoration of the stromal architecture	1918:1967	After six months, the wound area ECM morphology was similar to controls, but transmittance values remained low, denoting incomplete restoration of the stromal architecture.					
31228462	14	105	theme	collagen	1714:1721	arg1	fibers					1723:1728	collagen fibers	1714:1728	collagen fibers	1714:1728	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	3	106	theme	optical	424:430	arg1	transparency					432:443	optical transparency	424:443	optical transparency	424:443	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	0	107	theme	corneal	51:57	arg1	healing					65:71	corneal wound healing	51:71	corneal wound healing	51:71	Dynamic changes of the extracellular matrix during corneal wound healing.					
31228462	14	108	theme	type	1748:1751	arg1	levels					1766:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels	1640:1771	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	6	109	dep	burned	775:780	arg1	corneas					794:800	corneas	794:800	corneas	794:800	At each time point, the clinical conditions of the burned and control corneas were observed.					
31228462	6	109	dep	burned	775:780	arg1	the					771:773	the	771:773	the	771:773	At each time point, the clinical conditions of the burned and control corneas were observed.					
31228462	3	110	dep	location	327:334	arg1	the					323:325	the	323:325	the	323:325	We analyzed the location and expression of collagens and proteoglycans, the clinical impact, and the recovery of optical transparency.					
31228462	10	111	theme	mRNA	1096:1099	arg1	levels					1112:1117	mRNA expression levels	1096:1117	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin	1096:1204	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	17	112	used	used	2289:2292	arg2	information					2265:2275	reliable information	2256:2275	reliable information that can be used to test new therapies	2256:2314	Documenting wound resolution during the six-month period provided reliable information that can be used to test new therapies.					
31228462	16	113	theme	healing	2020:2026	arg1	process					2028:2034	the stromal wound healing process	2002:2034	the stromal wound healing process	2002:2034	This multidisciplinary study of the stromal wound healing process revealed changes in corneal transmittance, collagen organization, myofibroblasts presence and ECM composition at 1, 3, and 6 months after alkali burning.					
31228462	14	114	theme	fibers	1723:1728	arg1	type					1748:1751	only collagen type I	1734:1753	only collagen type I	1734:1753	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	14	114	theme	fibers	1723:1728	arg1	opacity					1656:1662	corneal opacity	1648:1662	corneal opacity	1648:1662	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	14	114	theme	fibers	1723:1728	arg1	reorganization					1696:1709	reorganization	1696:1709	reorganization of collagen fibers	1696:1728	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	14	114	theme	fibers	1723:1728	arg1	transmittance					1681:1693	increased light transmittance	1665:1693	increased light transmittance	1665:1693	After three months, wound healing progress was indicated by reduced corneal opacity, increased light transmittance, reorganization of collagen fibers and only collagen type I expression levels were at control levels.					
31228462	4	115	theme	8-mm	577:580	arg1	paper					598:602	an 8-mm diameter filter paper	574:602	an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s	574:632	After the animals received both general and ocular topical anesthesia, the central cornea of the left eye was burned by placing an 8-mm diameter filter paper soaked in 0.5 N NaOH for 60 s.					
31228462	1	116	theme	proteoglycans	218:230	arg1	organization					163:174	the precise organization	151:174	the precise organization of collagen fibrils	151:194	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	1	116	theme	proteoglycans	218:230	arg1	fibrils					188:194	collagen fibrils	179:194	collagen fibrils	179:194	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	1	116	theme	proteoglycans	218:230	arg1	proteoglycans					218:230	proteoglycans	218:230	proteoglycans	218:230	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	1	116	theme	proteoglycans	218:230	arg1	variety					207:213	a wide variety	200:213	a wide variety of proteoglycans	200:230	The extracellular matrix (ECM) confers transparency to the cornea because of the precise organization of collagen fibrils and a wide variety of proteoglycans.					
31228462	10	117	theme	collagen	1122:1129	arg1	I					1137:1137	I	1137:1137	I	1137:1137	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	117	theme	collagen	1122:1129	arg1	decorin					1157:1163	decorin	1157:1163	decorin	1157:1163	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	117	theme	collagen	1122:1129	arg1	keratocan					1166:1174	keratocan	1166:1174	keratocan	1166:1174	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	117	theme	collagen	1122:1129	arg1	III					1143:1145	III	1143:1145	III	1143:1145	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	117	theme	collagen	1122:1129	arg1	lumican					1148:1154	lumican	1148:1154	lumican	1148:1154	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	117	theme	collagen	1122:1129	arg1	actin					1200:1204	alpha-smooth muscle actin	1180:1204	alpha-smooth muscle actin	1180:1204	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
31228462	10	117	theme	collagen	1122:1129	arg1	types					1131:1135	collagen types I and III	1122:1145	collagen types I and III	1122:1145	mRNA expression levels of collagen types I and III, lumican, decorin, keratocan and alpha-smooth muscle actin were determined by quantitative real-time polymerase chain reaction.					
29452500	13	0	dep	4	1772:1772	arg1	lower					1786:1790	lower	1786:1790	lower	1786:1790	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	6	1	theme	conjugates	1000:1009	arg1	properties					986:995	physicochemical properties	970:995	physicochemical properties of conjugates	970:1009	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	1	2	theme	ciprofloxacin-loaded	281:300	arg1	formulation					302:312	an optimum ciprofloxacin-loaded formulation	270:312	an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa	270:440	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	12	3	theme	particle	1561:1568	arg1	size					1570:1573	particle size	1561:1573	particle size of about 260 nm	1561:1589	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	12	4	theme	high	1529:1532	arg1	%					1552:1552	≈ 19%	1548:1552	≈ 19%	1548:1552	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	12	4	theme	high	1529:1532	arg1	loading					1539:1545	high drug loading	1529:1545	high drug loading (≈ 19%)	1529:1553	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	4	5	theme	adverse	670:676	arg1	ciprofloxacin					724:736	ciprofloxacin	724:736	ciprofloxacin	724:736	By site-specific delivery of drug, the adverse effects of broad-spectrum antibiotics such as ciprofloxacin would be reduced.					
29452500	4	5	theme	adverse	670:676	arg1	effects					678:684	the adverse effects	666:684	the adverse effects of broad-spectrum antibiotics such as ciprofloxacin	666:736	By site-specific delivery of drug, the adverse effects of broad-spectrum antibiotics such as ciprofloxacin would be reduced.					
29452500	6	6	theme	acid	908:911	arg1	type					913:916	fatty acid type	902:916	fatty acid type (stearic acid, palmitic acid, and linoleic acid)	902:965	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	13	7	dep	K.	1745:1746	arg1	pneumoniae					1748:1757	K. pneumoniae	1745:1757	K. pneumoniae	1745:1757	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	3	8	theme	antibiotic	608:617	arg1	resistance					619:628	the antibiotic resistance	604:628	the antibiotic resistance	604:628	Nanocarriers can increase the therapeutic index and consequently reduce the antibiotic resistance.					
29452500	1	9	theme	structure	182:190	arg1	effect					166:171	the effect	162:171	the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles	162:256	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	1	10	from	conjugates	325:334	arg1	formulation					302:312	an optimum ciprofloxacin-loaded formulation	270:312	an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa	270:440	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	1	11	theme	physicochemical	195:209	arg1	properties					211:220	physicochemical properties	195:220	physicochemical properties of chitosan-fatty acid nanomicelles	195:256	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	6	12	dep	type	913:916	arg1	acid					961:964	linoleic acid	952:964	linoleic acid	952:964	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	6	12	dep	type	913:916	arg1	acid					927:930	stearic acid	919:930	stearic acid	919:930	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	6	12	dep	type	913:916	arg1	acid					942:945	palmitic acid	933:945	palmitic acid	933:945	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	13	13	theme	inhibitory	1653:1662	arg1	concentrations					1664:1677	The minimum inhibitory concentrations	1641:1677	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species	1641:1765	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	13	13	theme	inhibitory	1653:1662	arg1	4					1772:1772	4	1772:1772	4	1772:1772	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	1	14	theme	acid	240:243	arg1	nanomicelles					245:256	chitosan-fatty acid nanomicelles	225:256	chitosan-fatty acid nanomicelles	225:256	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	13	15	with	comparison	1795:1804	arg1	drug					1820:1823	the free drug	1811:1823	the free drug	1811:1823	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	1	16	theme	study	140:144	arg1	objective					122:130	The objective	118:130	The objective of this study	118:144	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	7	17	theme	hydration	1090:1098	arg1	method					1100:1105	thin film hydration method	1080:1105	thin film hydration method	1080:1105	Ciprofloxacin was encapsulated in nanomicelles by thin film hydration method.					
29452500	5	18	theme	3-dimethylaminopropyl	850:870	arg1	carbodiimide					873:884	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	839:884	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	839:884	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	7	19	theme	thin	1080:1083	arg1	method					1100:1105	thin film hydration method	1080:1105	thin film hydration method	1080:1105	Ciprofloxacin was encapsulated in nanomicelles by thin film hydration method.					
29452500	1	20	dep	OBJECTIVE	108:116	arg1	was					146:148	was	146:148	was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa	146:440	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	5	21	theme	carbodiimide	873:884	arg1	presence					827:834	the presence	823:834	the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	823:884	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	8	22	theme	preparation	1118:1128	arg1	process					1130:1136	the preparation process	1114:1136	the preparation process	1114:1136	Also, the preparation process was optimized with a central composite design.					
29452500	1	23	from	effect	166:171	arg1	properties					211:220	physicochemical properties	195:220	physicochemical properties of chitosan-fatty acid nanomicelles	195:256	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	6	24	theme	linoleic	952:959	arg1	acid					961:964	linoleic acid	952:964	linoleic acid	952:964	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	5	25	dep	grafted	775:781	arg1	chitosan					783:790	chitosan	783:790	chitosan	783:790	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	5	25	dep	grafted	775:781	arg1	acid					770:773	Fatty acid	764:773	METHODS Fatty acid grafted chitosan conjugates	756:801	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	5	25	dep	grafted	775:781	arg1	conjugates					792:801	conjugates	792:801	conjugates	792:801	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	10	26	theme	high	1358:1361	arg1	values					1369:1374	high yield values	1358:1374	high yield values	1358:1374	RESULTS All conjugates were synthetized with high yield values and the substitution degrees ranged between 2.13 and 35.46%.					
29452500	1	27	theme	important	403:411	arg1	pathogens					413:421	some important pathogens	398:421	some important pathogens like P. aeruginosa	398:440	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	9	28	dep	aeruginosa	1244:1253	arg1	species					1289:1295	species	1289:1295	species	1289:1295	The antibacterial effect of optimum formulation against P. aeruginosa, K. pneumoniae, and S. pneumoniae species was determined.					
29452500	5	29	theme	1-ethyl-3-	839:848	arg1	carbodiimide					873:884	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	839:884	1-ethyl-3-(3-dimethylaminopropyl) carbodiimide	839:884	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	2	30	theme	growing	505:511	arg1	SIGNIFICANCE					443:454	SIGNIFICANCE	443:454	SIGNIFICANCE Nowadays, resistance in infectious diseases	443:498	SIGNIFICANCE Nowadays, resistance in infectious diseases is a growing worldwide concern.					
29452500	2	30	theme	growing	505:511	arg1	concern					523:529	a growing worldwide concern	503:529	a growing worldwide concern	503:529	SIGNIFICANCE Nowadays, resistance in infectious diseases is a growing worldwide concern.					
29452500	9	31	theme	antibacterial	1189:1201	arg1	effect					1203:1208	The antibacterial effect	1185:1208	The antibacterial effect of optimum formulation against P. aeruginosa, K. pneumoniae, and S. pneumoniae species	1185:1295	The antibacterial effect of optimum formulation against P. aeruginosa, K. pneumoniae, and S. pneumoniae species was determined.					
29452500	14	32	theme	chitosan	1935:1942	arg1	nanomicelles					1944:1955	chitosan nanomicelles	1935:1955	chitosan nanomicelles	1935:1955	CONCLUSIONS The antibacterial effect of ciprofloxacin was improved by encapsulation of drug in chitosan nanomicelles.					
29452500	8	33	theme	central	1159:1165	arg1	design					1177:1182	a central composite design	1157:1182	a central composite design	1157:1182	Also, the preparation process was optimized with a central composite design.					
29452500	2	34	theme	infectious	480:489	arg1	diseases					491:498	infectious diseases	480:498	infectious diseases	480:498	SIGNIFICANCE Nowadays, resistance in infectious diseases is a growing worldwide concern.					
29452500	14	35	theme	drug	1927:1930	arg1	encapsulation					1910:1922	encapsulation	1910:1922	encapsulation of drug in chitosan nanomicelles	1910:1955	CONCLUSIONS The antibacterial effect of ciprofloxacin was improved by encapsulation of drug in chitosan nanomicelles.					
29452500	12	36	theme	sustained	1597:1605	arg1	profile					1615:1621	a sustained release profile	1595:1621	a sustained release profile of ciprofloxacin	1595:1638	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	9	37	theme	formulation	1221:1231	arg1	effect					1203:1208	The antibacterial effect	1185:1208	The antibacterial effect of optimum formulation against P. aeruginosa, K. pneumoniae, and S. pneumoniae species	1185:1295	The antibacterial effect of optimum formulation against P. aeruginosa, K. pneumoniae, and S. pneumoniae species was determined.					
29452500	0	38	theme	ciprofloxacin-loaded	28:47	arg1	chitosan					49:56	an optimized ciprofloxacin-loaded chitosan	15:56	an optimized ciprofloxacin-loaded chitosan	15:56	Preparation of an optimized ciprofloxacin-loaded chitosan nanomicelle with enhanced antibacterial activity.					
29452500	6	39	from	effects	891:897	arg1	properties					986:995	physicochemical properties	970:995	physicochemical properties of conjugates	970:1009	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	0	40	theme	enhanced	75:82	arg1	activity					98:105	enhanced antibacterial activity	75:105	enhanced antibacterial activity	75:105	Preparation of an optimized ciprofloxacin-loaded chitosan nanomicelle with enhanced antibacterial activity.					
29452500	1	41	theme	antibacterial	360:372	arg1	effects					374:380	the antibacterial effects	356:380	the antibacterial effects of drug against some important pathogens like P. aeruginosa	356:440	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	6	42	theme	palmitic	933:940	arg1	acid					942:945	palmitic acid	933:945	palmitic acid	933:945	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	1	43	theme	nanomicelles	245:256	arg1	properties					211:220	physicochemical properties	195:220	physicochemical properties of chitosan-fatty acid nanomicelles	195:256	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	2	44	dep	Nowadays	456:463	arg1	resistance					466:475	resistance	466:475	resistance	466:475	SIGNIFICANCE Nowadays, resistance in infectious diseases is a growing worldwide concern.					
29452500	4	45	theme	broad-spectrum	689:702	arg1	antibiotics					704:714	broad-spectrum antibiotics	689:714	broad-spectrum antibiotics	689:714	By site-specific delivery of drug, the adverse effects of broad-spectrum antibiotics such as ciprofloxacin would be reduced.					
29452500	1	46	theme	optimum	273:279	arg1	formulation					302:312	an optimum ciprofloxacin-loaded formulation	270:312	an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa	270:440	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	12	47	theme	release	1607:1613	arg1	profile					1615:1621	a sustained release profile	1595:1621	a sustained release profile of ciprofloxacin	1595:1638	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	4	48	theme	drug	660:663	arg1	delivery					648:655	site-specific delivery	634:655	site-specific delivery of drug	634:663	By site-specific delivery of drug, the adverse effects of broad-spectrum antibiotics such as ciprofloxacin would be reduced.					
29452500	6	49	theme	type	913:916	arg1	effects					891:897	The effects	887:897	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates	887:1009	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	13	50	from	4	1772:1772	arg1	comparison					1795:1804	comparison	1795:1804	comparison with the free drug	1795:1823	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	6	51	theme	physicochemical	970:984	arg1	properties					986:995	physicochemical properties	970:995	physicochemical properties of conjugates	970:1009	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	6	52	theme	fatty	902:906	arg1	type					913:916	fatty acid type	902:916	fatty acid type (stearic acid, palmitic acid, and linoleic acid)	902:965	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	1	53	theme	lipid	176:180	arg1	structure					182:190	lipid structure	176:190	lipid structure	176:190	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	12	54	theme	drug	1534:1537	arg1	%					1552:1552	≈ 19%	1548:1552	≈ 19%	1548:1552	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	12	54	theme	drug	1534:1537	arg1	loading					1539:1545	high drug loading	1529:1545	high drug loading (≈ 19%)	1529:1553	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	13	55	theme	ciprofloxacin	1682:1694	arg1	concentrations					1664:1677	The minimum inhibitory concentrations	1641:1677	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species	1641:1765	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	13	55	theme	ciprofloxacin	1682:1694	arg1	4					1772:1772	4	1772:1772	4	1772:1772	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	13	56	from	concentrations	1664:1677	arg1	formulation					1707:1717	optimum formulation	1699:1717	optimum formulation against P. aeruginosa and K. pneumoniae species	1699:1765	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	13	57	theme	optimum	1699:1705	arg1	formulation					1707:1717	optimum formulation	1699:1717	optimum formulation against P. aeruginosa and K. pneumoniae species	1699:1765	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	12	58	theme	optimum	1502:1508	arg1	formulation					1510:1520	The optimum formulation	1498:1520	The optimum formulation	1498:1520	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	13	59	theme	minimum	1645:1651	arg1	concentrations					1664:1677	The minimum inhibitory concentrations	1641:1677	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species	1641:1765	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	13	59	theme	minimum	1645:1651	arg1	4					1772:1772	4	1772:1772	4	1772:1772	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	1	60	theme	chitosan-fatty	225:238	arg1	nanomicelles					245:256	chitosan-fatty acid nanomicelles	225:256	chitosan-fatty acid nanomicelles	225:256	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	5	61	theme	Fatty	764:768	arg1	chitosan					783:790	chitosan	783:790	chitosan	783:790	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	5	61	theme	Fatty	764:768	arg1	acid					770:773	Fatty acid	764:773	METHODS Fatty acid grafted chitosan conjugates	756:801	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	5	61	theme	Fatty	764:768	arg1	conjugates					792:801	conjugates	792:801	conjugates	792:801	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	0	62	theme	antibacterial	84:96	arg1	activity					98:105	enhanced antibacterial activity	75:105	enhanced antibacterial activity	75:105	Preparation of an optimized ciprofloxacin-loaded chitosan nanomicelle with enhanced antibacterial activity.					
29452500	14	63	theme	ciprofloxacin	1880:1892	arg1	effect					1870:1875	The antibacterial effect	1852:1875	The antibacterial effect of ciprofloxacin	1852:1892	CONCLUSIONS The antibacterial effect of ciprofloxacin was improved by encapsulation of drug in chitosan nanomicelles.					
29452500	14	64	dep	CONCLUSIONS	1840:1850	arg1	improved					1898:1905	improved	1898:1905	was improved by encapsulation of drug in chitosan nanomicelles	1894:1955	CONCLUSIONS The antibacterial effect of ciprofloxacin was improved by encapsulation of drug in chitosan nanomicelles.					
29452500	1	65	theme	drug	385:388	arg1	effects					374:380	the antibacterial effects	356:380	the antibacterial effects of drug against some important pathogens like P. aeruginosa	356:440	OBJECTIVE The objective of this study was to evaluate the effect of lipid structure on physicochemical properties of chitosan-fatty acid nanomicelles and prepare an optimum ciprofloxacin-loaded formulation from these conjugates which could enhance the antibacterial effects of drug against some important pathogens like P. aeruginosa.					
29452500	7	66	theme	film	1085:1088	arg1	method					1100:1105	thin film hydration method	1080:1105	thin film hydration method	1080:1105	Ciprofloxacin was encapsulated in nanomicelles by thin film hydration method.					
29452500	12	67	theme	ciprofloxacin	1626:1638	arg1	profile					1615:1621	a sustained release profile	1595:1621	a sustained release profile of ciprofloxacin	1595:1638	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	12	67	theme	ciprofloxacin	1626:1638	arg1	size					1570:1573	particle size	1561:1573	particle size of about 260 nm	1561:1589	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	13	68	theme	free	1815:1818	arg1	drug					1820:1823	the free drug	1811:1823	the free drug	1811:1823	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	10	69	theme	yield	1363:1367	arg1	values					1369:1374	high yield values	1358:1374	high yield values	1358:1374	RESULTS All conjugates were synthetized with high yield values and the substitution degrees ranged between 2.13 and 35.46%.					
29452500	10	70	theme	substitution	1384:1395	arg1	degrees					1397:1403	the substitution degrees	1380:1403	the substitution degrees	1380:1403	RESULTS All conjugates were synthetized with high yield values and the substitution degrees ranged between 2.13 and 35.46%.					
29452500	8	71	theme	composite	1167:1175	arg1	design					1177:1182	a central composite design	1157:1182	a central composite design	1157:1182	Also, the preparation process was optimized with a central composite design.					
29452500	5	72	dep	METHODS	756:762	arg1	chitosan					783:790	chitosan	783:790	chitosan	783:790	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	5	72	dep	METHODS	756:762	arg1	acid					770:773	Fatty acid	764:773	METHODS Fatty acid grafted chitosan conjugates	756:801	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	5	72	dep	METHODS	756:762	arg1	conjugates					792:801	conjugates	792:801	conjugates	792:801	METHODS Fatty acid grafted chitosan conjugates were synthetized in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide.					
29452500	4	73	theme	site-specific	634:646	arg1	delivery					648:655	site-specific delivery	634:655	site-specific delivery of drug	634:663	By site-specific delivery of drug, the adverse effects of broad-spectrum antibiotics such as ciprofloxacin would be reduced.					
29452500	6	74	theme	stearic	919:925	arg1	acid					927:930	stearic acid	919:930	stearic acid	919:930	The effects of fatty acid type (stearic acid, palmitic acid, and linoleic acid) on physicochemical properties of conjugates were investigated.					
29452500	0	75	theme	optimized	18:26	arg1	chitosan					49:56	an optimized ciprofloxacin-loaded chitosan	15:56	an optimized ciprofloxacin-loaded chitosan	15:56	Preparation of an optimized ciprofloxacin-loaded chitosan nanomicelle with enhanced antibacterial activity.					
29452500	9	76	theme	optimum	1213:1219	arg1	formulation					1221:1231	optimum formulation	1213:1231	optimum formulation	1213:1231	The antibacterial effect of optimum formulation against P. aeruginosa, K. pneumoniae, and S. pneumoniae species was determined.					
29452500	0	77	theme	chitosan	49:56	arg1	Preparation					0:10	Preparation	0:10	Preparation of an optimized ciprofloxacin-loaded chitosan	0:56	Preparation of an optimized ciprofloxacin-loaded chitosan nanomicelle with enhanced antibacterial activity.					
29452500	14	78	from	encapsulation	1910:1922	arg1	nanomicelles					1944:1955	chitosan nanomicelles	1935:1955	chitosan nanomicelles	1935:1955	CONCLUSIONS The antibacterial effect of ciprofloxacin was improved by encapsulation of drug in chitosan nanomicelles.					
29452500	10	79	dep	RESULTS	1313:1319	arg1	synthetized					1341:1351	synthetized	1341:1351	were synthetized with high yield values and the substitution degrees ranged between 2.13 and 35.46%	1336:1434	RESULTS All conjugates were synthetized with high yield values and the substitution degrees ranged between 2.13 and 35.46%.					
29452500	13	80	dep	aeruginosa	1730:1739	arg1	species					1759:1765	species	1759:1765	species	1759:1765	The minimum inhibitory concentrations of ciprofloxacin in optimum formulation against P. aeruginosa and K. pneumoniae species were 4 and 2 times lower in comparison with the free drug, respectively.					
29452500	2	81	theme	worldwide	513:521	arg1	SIGNIFICANCE					443:454	SIGNIFICANCE	443:454	SIGNIFICANCE Nowadays, resistance in infectious diseases	443:498	SIGNIFICANCE Nowadays, resistance in infectious diseases is a growing worldwide concern.					
29452500	2	81	theme	worldwide	513:521	arg1	concern					523:529	a growing worldwide concern	503:529	a growing worldwide concern	503:529	SIGNIFICANCE Nowadays, resistance in infectious diseases is a growing worldwide concern.					
29452500	3	82	theme	therapeutic	562:572	arg1	index					574:578	the therapeutic index	558:578	the therapeutic index	558:578	Nanocarriers can increase the therapeutic index and consequently reduce the antibiotic resistance.					
29452500	2	83	from	SIGNIFICANCE	443:454	arg1	diseases					491:498	infectious diseases	480:498	infectious diseases	480:498	SIGNIFICANCE Nowadays, resistance in infectious diseases is a growing worldwide concern.					
29452500	12	84	theme	260 nm	1584:1589	arg1	profile					1615:1621	a sustained release profile	1595:1621	a sustained release profile of ciprofloxacin	1595:1638	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	12	84	theme	260 nm	1584:1589	arg1	size					1570:1573	particle size	1561:1573	particle size of about 260 nm	1561:1589	The optimum formulation showed high drug loading (≈ 19%), with particle size of about 260 nm and a sustained release profile of ciprofloxacin.					
29452500	4	85	theme	antibiotics	704:714	arg1	ciprofloxacin					724:736	ciprofloxacin	724:736	ciprofloxacin	724:736	By site-specific delivery of drug, the adverse effects of broad-spectrum antibiotics such as ciprofloxacin would be reduced.					
29452500	4	85	theme	antibiotics	704:714	arg1	effects					678:684	the adverse effects	666:684	the adverse effects of broad-spectrum antibiotics such as ciprofloxacin	666:736	By site-specific delivery of drug, the adverse effects of broad-spectrum antibiotics such as ciprofloxacin would be reduced.					
29452500	2	86	dep	SIGNIFICANCE	443:454	arg1	Nowadays					456:463	Nowadays	456:463	Nowadays	456:463	SIGNIFICANCE Nowadays, resistance in infectious diseases is a growing worldwide concern.					
29452500	14	87	theme	antibacterial	1856:1868	arg1	effect					1870:1875	The antibacterial effect	1852:1875	The antibacterial effect of ciprofloxacin	1852:1892	CONCLUSIONS The antibacterial effect of ciprofloxacin was improved by encapsulation of drug in chitosan nanomicelles.					
31544955	6	0	theme	time	977:980	arg1	points					982:987	various time points	969:987	various time points for pharmacokinetic analyses	969:1016	CD-1 mice were intravenously injected with the 23BChE or 26BChE, and residual BChE activities from blood collected at various time points for pharmacokinetic analyses.					
31544955	7	1	theme	lower	1056:1060	arg1	level					1082:1086	a slightly lower initial sialylation level	1045:1086	a slightly lower initial sialylation level	1045:1086	Although 23BChE contained a slightly lower initial sialylation level compared to 26BChE, the molecule exhibited higher residual activity between 5 and 24 hr postinjection.					
31544955	3	2	theme	α-2,6	536:540	arg1	enzyme					569:574	the α-2,6 sialyltransferase (26BChE) enzyme	532:574	the α-2,6 sialyltransferase (26BChE) enzyme	532:574	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	8	3	theme	Pharmacokinetic	1191:1205	arg1	analyses					1207:1214	Pharmacokinetic analyses	1191:1214	Pharmacokinetic analyses	1191:1214	Pharmacokinetic analyses indicated that 23BChE exhibited an increase in area under the curve and a lower volume of distribution at steady state than that of 26BChE.					
31544955	9	4	theme	biotherapeutic	1481:1494	arg1	behavior					1467:1474	the pharmacokinetic behavior	1447:1474	the pharmacokinetic behavior of a biotherapeutic	1447:1494	These findings suggest that the type of sialylation linkage may play a significant role in the pharmacokinetic behavior of a biotherapeutic when tested in in vivo animal models.					
31544955	0	5	theme	butyrylcholinesterases	78:99	arg1	pharmacokinetics					46:61	the pharmacokinetics	42:61	the pharmacokinetics of recombinant butyrylcholinesterases	42:99	The impact of sialylation linkage-type on the pharmacokinetics of recombinant butyrylcholinesterases.					
31544955	0	6	from	impact	4:9	arg1	pharmacokinetics					46:61	the pharmacokinetics	42:61	the pharmacokinetics of recombinant butyrylcholinesterases	42:99	The impact of sialylation linkage-type on the pharmacokinetics of recombinant butyrylcholinesterases.					
31544955	4	7	attach	derived	665:671	arg2	23BChE					657:662	23BChE	657:662	23BChE	657:662	The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.					
31544955	4	7	attach	derived	665:671	arg2	sialylation					644:654	α-2,3 sialylation	638:654	α-2,3 sialylation (23BChE) derived from wild-type CHO cells	638:696	The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.					
31544955	4	7	attach	derived	665:671	arg1	cells					692:696	wild-type CHO cells	678:696	wild-type CHO cells	678:696	The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.					
31544955	3	8	theme	sialyltransferase	542:558	arg1	enzyme					569:574	the α-2,6 sialyltransferase (26BChE) enzyme	532:574	the α-2,6 sialyltransferase (26BChE) enzyme	532:574	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	3	9	theme	human	391:395	arg1	BChE					420:423	BChE	420:423	BChE	420:423	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	3	9	theme	human	391:395	arg1	butyrylcholinesterase					397:417	recombinant human butyrylcholinesterase	379:417	recombinant human butyrylcholinesterase (BChE)	379:424	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	6	10	theme	CD-1	851:854	arg1	mice					856:859	CD-1 mice	851:859	CD-1 mice	851:859	CD-1 mice were intravenously injected with the 23BChE or 26BChE, and residual BChE activities from blood collected at various time points for pharmacokinetic analyses.					
31544955	5	11	theme	bi-antennary	774:785	arg1	glycans					801:807	bi-antennary di-sialylated glycans	774:807	bi-antennary di-sialylated glycans representing the most abundant glycoform	774:848	Both 23BChE and 26BChE exhibited comparable antennarity distributions with bi-antennary di-sialylated glycans representing the most abundant glycoform.					
31544955	5	12	theme	comparable	732:741	arg1	distributions					755:767	comparable antennarity distributions	732:767	comparable antennarity distributions	732:767	Both 23BChE and 26BChE exhibited comparable antennarity distributions with bi-antennary di-sialylated glycans representing the most abundant glycoform.					
31544955	1	13	with	glycoproteins	154:166	arg1	N-glycans					173:181	N-glycans	173:181	N-glycans terminating in α-2,3 sialylation	173:214	Chinese hamster ovary (CHO) cells typically produce glycoproteins with N-glycans terminating in α-2,3 sialylation.					
31544955	5	14	theme	di-sialylated	787:799	arg1	glycans					801:807	bi-antennary di-sialylated glycans	774:807	bi-antennary di-sialylated glycans representing the most abundant glycoform	774:848	Both 23BChE and 26BChE exhibited comparable antennarity distributions with bi-antennary di-sialylated glycans representing the most abundant glycoform.					
31544955	9	15	theme	linkage	1408:1414	arg1	type					1388:1391	the type	1384:1391	the type of sialylation linkage	1384:1414	These findings suggest that the type of sialylation linkage may play a significant role in the pharmacokinetic behavior of a biotherapeutic when tested in in vivo animal models.					
31544955	9	16	theme	animal	1519:1524	arg1	models					1526:1531	in vivo animal models	1511:1531	in vivo animal models	1511:1531	These findings suggest that the type of sialylation linkage may play a significant role in the pharmacokinetic behavior of a biotherapeutic when tested in in vivo animal models.					
31544955	4	17	theme	CHO	688:690	arg1	cells					692:696	wild-type CHO cells	678:696	wild-type CHO cells	678:696	The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.					
31544955	3	18	theme	recombinant	379:389	arg1	BChE					420:423	BChE	420:423	BChE	420:423	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	3	18	theme	recombinant	379:389	arg1	butyrylcholinesterase					397:417	recombinant human butyrylcholinesterase	379:417	recombinant human butyrylcholinesterase (BChE)	379:424	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	8	19	from	volume	1296:1301	arg1	area					1263:1266	area	1263:1266	area under the curve	1263:1282	Pharmacokinetic analyses indicated that 23BChE exhibited an increase in area under the curve and a lower volume of distribution at steady state than that of 26BChE.					
31544955	3	20	theme	enzyme	569:574	arg1	overexpression					514:527	overexpression	514:527	overexpression of the α-2,6 sialyltransferase (26BChE) enzyme	514:574	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	4	21	theme	wild-type	678:686	arg1	cells					692:696	wild-type CHO cells	678:696	wild-type CHO cells	678:696	The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.					
31544955	5	22	theme	abundant	831:838	arg1	glycoform					840:848	the most abundant glycoform	822:848	the most abundant glycoform	822:848	Both 23BChE and 26BChE exhibited comparable antennarity distributions with bi-antennary di-sialylated glycans representing the most abundant glycoform.					
31544955	3	23	theme	pharmacokinetic	351:365	arg1	properties					367:376	pharmacokinetic properties	351:376	pharmacokinetic properties	351:376	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	1	24	theme	Chinese	102:108	arg1	CHO					125:127	CHO	125:127	CHO	125:127	Chinese hamster ovary (CHO) cells typically produce glycoproteins with N-glycans terminating in α-2,3 sialylation.					
31544955	1	24	theme	Chinese	102:108	arg1	ovary					118:122	Chinese hamster ovary	102:122	Chinese hamster ovary (CHO) cells	102:134	Chinese hamster ovary (CHO) cells typically produce glycoproteins with N-glycans terminating in α-2,3 sialylation.					
31544955	1	25	theme	α-2,3	198:202	arg1	sialylation					204:214	α-2,3 sialylation	198:214	α-2,3 sialylation	198:214	Chinese hamster ovary (CHO) cells typically produce glycoproteins with N-glycans terminating in α-2,3 sialylation.					
31544955	6	26	theme	various	969:975	arg1	points					982:987	various time points	969:987	various time points for pharmacokinetic analyses	969:1016	CD-1 mice were intravenously injected with the 23BChE or 26BChE, and residual BChE activities from blood collected at various time points for pharmacokinetic analyses.					
31544955	6	27	dep	23BChE	898:903	arg1	the					894:896	the	894:896	the	894:896	CD-1 mice were intravenously injected with the 23BChE or 26BChE, and residual BChE activities from blood collected at various time points for pharmacokinetic analyses.					
31544955	8	28	theme	steady	1322:1327	arg1	state					1329:1333	steady state	1322:1333	steady state	1322:1333	Pharmacokinetic analyses indicated that 23BChE exhibited an increase in area under the curve and a lower volume of distribution at steady state than that of 26BChE.					
31544955	1	29	theme	hamster	110:116	arg1	CHO					125:127	CHO	125:127	CHO	125:127	Chinese hamster ovary (CHO) cells typically produce glycoproteins with N-glycans terminating in α-2,3 sialylation.					
31544955	1	29	theme	hamster	110:116	arg1	ovary					118:122	Chinese hamster ovary	102:122	Chinese hamster ovary (CHO) cells	102:134	Chinese hamster ovary (CHO) cells typically produce glycoproteins with N-glycans terminating in α-2,3 sialylation.					
31544955	0	30	theme	sialylation	14:24	arg1	impact					4:9	The impact	0:9	The impact of sialylation linkage-type on the pharmacokinetics of recombinant butyrylcholinesterases	0:99	The impact of sialylation linkage-type on the pharmacokinetics of recombinant butyrylcholinesterases.					
31544955	4	31	theme	N-glycan	581:588	arg1	composition					590:600	The N-glycan composition	577:600	The N-glycan composition of 26BChE	577:610	The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.					
31544955	9	32	theme	in	1511:1512	arg1	models					1526:1531	in vivo animal models	1511:1531	in vivo animal models	1511:1531	These findings suggest that the type of sialylation linkage may play a significant role in the pharmacokinetic behavior of a biotherapeutic when tested in in vivo animal models.					
31544955	1	33	theme	ovary	118:122	arg1	cells					130:134	Chinese hamster ovary (CHO) cells	102:134	Chinese hamster ovary (CHO) cells	102:134	Chinese hamster ovary (CHO) cells typically produce glycoproteins with N-glycans terminating in α-2,3 sialylation.					
31544955	4	34	theme	26BChE	605:610	arg1	composition					590:600	The N-glycan composition	577:600	The N-glycan composition of 26BChE	577:610	The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.					
31544955	4	35	theme	α-2,3	638:642	arg1	23BChE					657:662	23BChE	657:662	23BChE	657:662	The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.					
31544955	4	35	theme	α-2,3	638:642	arg1	sialylation					644:654	α-2,3 sialylation	638:654	α-2,3 sialylation (23BChE) derived from wild-type CHO cells	638:696	The N-glycan composition of 26BChE was compared to BChE with α-2,3 sialylation (23BChE) derived from wild-type CHO cells.					
31544955	6	36	theme	BChE	929:932	arg1	activities					934:943	residual BChE activities	920:943	residual BChE activities	920:943	CD-1 mice were intravenously injected with the 23BChE or 26BChE, and residual BChE activities from blood collected at various time points for pharmacokinetic analyses.					
31544955	5	37	gly	di-sialylated	787:799	arg1	glycans					801:807	bi-antennary di-sialylated glycans	774:807	bi-antennary di-sialylated glycans representing the most abundant glycoform	774:848	Both 23BChE and 26BChE exhibited comparable antennarity distributions with bi-antennary di-sialylated glycans representing the most abundant glycoform.					
31544955	3	38	theme	CHO	442:444	arg1	cells					446:450	CHO cells	442:450	CHO cells	442:450	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	5	39	theme	antennarity	743:753	arg1	distributions					755:767	comparable antennarity distributions	732:767	comparable antennarity distributions	732:767	Both 23BChE and 26BChE exhibited comparable antennarity distributions with bi-antennary di-sialylated glycans representing the most abundant glycoform.					
31544955	6	40	theme	residual	920:927	arg1	activities					934:943	residual BChE activities	920:943	residual BChE activities	920:943	CD-1 mice were intravenously injected with the 23BChE or 26BChE, and residual BChE activities from blood collected at various time points for pharmacokinetic analyses.					
31544955	2	41	theme	α-2,3	264:268	arg1	acids					287:291	α-2,3 and α-2,6 sialic acids	264:291	α-2,3 and α-2,6 sialic acids	264:291	Human cells produce glycoproteins that include α-2,3 and α-2,6 sialic acids.					
31544955	3	42	theme	α-2,3	472:476	arg1	genes					496:500	the α-2,3 sialyltransferase genes	468:500	the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme	468:574	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	0	43	theme	linkage-type	26:37	arg1	sialylation					14:24	sialylation linkage-type	14:37	sialylation linkage-type	14:37	The impact of sialylation linkage-type on the pharmacokinetics of recombinant butyrylcholinesterases.					
31544955	9	44	theme	significant	1427:1437	arg1	role					1439:1442	a significant role	1425:1442	a significant role	1425:1442	These findings suggest that the type of sialylation linkage may play a significant role in the pharmacokinetic behavior of a biotherapeutic when tested in in vivo animal models.					
31544955	7	45	theme	residual	1138:1145	arg1	activity					1147:1154	higher residual activity	1131:1154	higher residual activity	1131:1154	Although 23BChE contained a slightly lower initial sialylation level compared to 26BChE, the molecule exhibited higher residual activity between 5 and 24 hr postinjection.					
31544955	3	46	theme	sialyltransferase	478:494	arg1	genes					496:500	the α-2,3 sialyltransferase genes	468:500	the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme	468:574	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	2	47	gly	glycoproteins	237:249	arg1	glycoproteins					237:249	glycoproteins	237:249	glycoproteins that include α-2,3 and α-2,6 sialic acids	237:291	Human cells produce glycoproteins that include α-2,3 and α-2,6 sialic acids.					
31544955	1	48	gly	glycoproteins	154:166	arg1	glycoproteins					154:166	glycoproteins	154:166	glycoproteins with N-glycans terminating in α-2,3 sialylation	154:214	Chinese hamster ovary (CHO) cells typically produce glycoproteins with N-glycans terminating in α-2,3 sialylation.					
31544955	9	49	dep	in	1511:1512	arg1	vivo					1514:1517	vivo	1514:1517	vivo	1514:1517	These findings suggest that the type of sialylation linkage may play a significant role in the pharmacokinetic behavior of a biotherapeutic when tested in in vivo animal models.					
31544955	7	50	contain	contained	1035:1043	arg1	23BChE					1028:1033	23BChE	1028:1033	23BChE	1028:1033	Although 23BChE contained a slightly lower initial sialylation level compared to 26BChE, the molecule exhibited higher residual activity between 5 and 24 hr postinjection.					
31544955	7	50	contain	contained	1035:1043	arg2	level					1082:1086	a slightly lower initial sialylation level	1045:1086	a slightly lower initial sialylation level	1045:1086	Although 23BChE contained a slightly lower initial sialylation level compared to 26BChE, the molecule exhibited higher residual activity between 5 and 24 hr postinjection.					
31544955	6	51	theme	pharmacokinetic	993:1007	arg1	analyses					1009:1016	pharmacokinetic analyses	993:1016	pharmacokinetic analyses	993:1016	CD-1 mice were intravenously injected with the 23BChE or 26BChE, and residual BChE activities from blood collected at various time points for pharmacokinetic analyses.					
31544955	3	52	theme	protein	328:334	arg1	sialylation					336:346	protein sialylation	328:346	protein sialylation	328:346	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	9	53	theme	pharmacokinetic	1451:1465	arg1	behavior					1467:1474	the pharmacokinetic behavior	1447:1474	the pharmacokinetic behavior of a biotherapeutic	1447:1494	These findings suggest that the type of sialylation linkage may play a significant role in the pharmacokinetic behavior of a biotherapeutic when tested in in vivo animal models.					
31544955	3	54	from	impact	309:314	arg1	properties					367:376	pharmacokinetic properties	351:376	pharmacokinetic properties	351:376	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31544955	2	55	theme	Human	217:221	arg1	cells					223:227	Human cells	217:227	Human cells	217:227	Human cells produce glycoproteins that include α-2,3 and α-2,6 sialic acids.					
31544955	2	56	theme	sialic	280:285	arg1	acids					287:291	α-2,3 and α-2,6 sialic acids	264:291	α-2,3 and α-2,6 sialic acids	264:291	Human cells produce glycoproteins that include α-2,3 and α-2,6 sialic acids.					
31544955	7	57	theme	initial	1062:1068	arg1	level					1082:1086	a slightly lower initial sialylation level	1045:1086	a slightly lower initial sialylation level	1045:1086	Although 23BChE contained a slightly lower initial sialylation level compared to 26BChE, the molecule exhibited higher residual activity between 5 and 24 hr postinjection.					
31544955	9	58	theme	sialylation	1396:1406	arg1	linkage					1408:1414	sialylation linkage	1396:1414	sialylation linkage	1396:1414	These findings suggest that the type of sialylation linkage may play a significant role in the pharmacokinetic behavior of a biotherapeutic when tested in in vivo animal models.					
31544955	8	59	theme	lower	1290:1294	arg1	volume					1296:1301	a lower volume	1288:1301	a lower volume of distribution	1288:1317	Pharmacokinetic analyses indicated that 23BChE exhibited an increase in area under the curve and a lower volume of distribution at steady state than that of 26BChE.					
31544955	0	60	theme	recombinant	66:76	arg1	butyrylcholinesterases					78:99	recombinant butyrylcholinesterases	66:99	recombinant butyrylcholinesterases	66:99	The impact of sialylation linkage-type on the pharmacokinetics of recombinant butyrylcholinesterases.					
31544955	8	61	from	increase	1251:1258	arg1	area					1263:1266	area	1263:1266	area under the curve	1263:1282	Pharmacokinetic analyses indicated that 23BChE exhibited an increase in area under the curve and a lower volume of distribution at steady state than that of 26BChE.					
31544955	2	62	theme	α-2,6	274:278	arg1	acids					287:291	α-2,3 and α-2,6 sialic acids	264:291	α-2,3 and α-2,6 sialic acids	264:291	Human cells produce glycoproteins that include α-2,3 and α-2,6 sialic acids.					
31544955	7	63	theme	sialylation	1070:1080	arg1	level					1082:1086	a slightly lower initial sialylation level	1045:1086	a slightly lower initial sialylation level	1045:1086	Although 23BChE contained a slightly lower initial sialylation level compared to 26BChE, the molecule exhibited higher residual activity between 5 and 24 hr postinjection.					
31544955	8	64	theme	distribution	1306:1317	arg1	volume					1296:1301	a lower volume	1288:1301	a lower volume of distribution	1288:1317	Pharmacokinetic analyses indicated that 23BChE exhibited an increase in area under the curve and a lower volume of distribution at steady state than that of 26BChE.					
31544955	8	64	theme	distribution	1306:1317	arg1	increase					1251:1258	an increase	1248:1258	an increase in area under the curve	1248:1282	Pharmacokinetic analyses indicated that 23BChE exhibited an increase in area under the curve and a lower volume of distribution at steady state than that of 26BChE.					
31544955	7	65	theme	higher	1131:1136	arg1	activity					1147:1154	higher residual activity	1131:1154	higher residual activity	1131:1154	Although 23BChE contained a slightly lower initial sialylation level compared to 26BChE, the molecule exhibited higher residual activity between 5 and 24 hr postinjection.					
31544955	3	66	theme	26BChE	561:566	arg1	enzyme					569:574	the α-2,6 sialyltransferase (26BChE) enzyme	532:574	the α-2,6 sialyltransferase (26BChE) enzyme	532:574	To examine the impact of altering protein sialylation on pharmacokinetic properties, recombinant human butyrylcholinesterase (BChE) was produced in CHO cells by knocking out the α-2,3 sialyltransferase genes followed by overexpression of the α-2,6 sialyltransferase (26BChE) enzyme.					
31366552	0	0	theme	Hypertensive	81:92	arg1	Model					98:102	a Hypertensive Rat Model	79:102	a Hypertensive Rat Model	79:102	Preventive Effect of Oral Administration of Pantoea agglomerans-derived LPS in a Hypertensive Rat Model Upon Salt Loading.					
31366552	9	1	theme	preventive	1070:1079	arg1	effect					1081:1086	a preventive effect	1068:1086	a preventive effect on salt-loaded hypertension	1068:1114	CONCLUSION Oral administration of LPSp showed a preventive effect on salt-loaded hypertension.					
31366552	1	2	theme	Pantoea	161:167	arg1	LPSp					209:212	LPSp	209:212	LPSp	209:212	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	1	2	theme	Pantoea	161:167	arg1	lipopolysaccharide					189:206	Pantoea agglomerans-derived lipopolysaccharide	161:206	Pantoea agglomerans-derived lipopolysaccharide (LPSp)	161:213	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	2	3	theme	arm	401:403	arg1	kind					385:388	a kind	383:388	a kind of reserve arm for lifestyle-related diseases	383:434	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	2	3	theme	arm	401:403	arg1	pressure					364:371	high blood pressure	353:371	high blood pressure	353:371	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	1	4	theme	agglomerans-derived	169:187	arg1	LPSp					209:212	LPSp	209:212	LPSp	209:212	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	1	4	theme	agglomerans-derived	169:187	arg1	lipopolysaccharide					189:206	Pantoea agglomerans-derived lipopolysaccharide	161:206	Pantoea agglomerans-derived lipopolysaccharide (LPSp)	161:213	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	2	5	from	effect	343:348	arg1	kind					385:388	a kind	383:388	a kind of reserve arm for lifestyle-related diseases	383:434	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	2	5	from	effect	343:348	arg1	pressure					364:371	high blood pressure	353:371	high blood pressure	353:371	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	1	6	theme	various	269:275	arg1	diseases					295:302	various lifestyle-related diseases	269:302	various lifestyle-related diseases	269:302	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	7	7	theme	no	895:896	arg1	reduction					898:906	almost no reduction	888:906	almost no reduction	888:906	Although there was almost no reduction in blood pressure in SHRs that received LPSp.					
31366552	1	8	theme	lifestyle-related	277:293	arg1	diseases					295:302	various lifestyle-related diseases	269:302	various lifestyle-related diseases	269:302	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	0	9	theme	Rat	94:96	arg1	Model					98:102	a Hypertensive Rat Model	79:102	a Hypertensive Rat Model	79:102	Preventive Effect of Oral Administration of Pantoea agglomerans-derived LPS in a Hypertensive Rat Model Upon Salt Loading.					
31366552	9	10	theme	salt-loaded	1091:1101	arg1	hypertension					1103:1114	salt-loaded hypertension	1091:1114	salt-loaded hypertension	1091:1114	CONCLUSION Oral administration of LPSp showed a preventive effect on salt-loaded hypertension.					
31366552	9	11	from	effect	1081:1086	arg1	hypertension					1103:1114	salt-loaded hypertension	1091:1114	salt-loaded hypertension	1091:1114	CONCLUSION Oral administration of LPSp showed a preventive effect on salt-loaded hypertension.					
31366552	4	12	theme	100	572:574	arg1	μg/kg					576:580	μg/kg	576:580	μg/kg	576:580	SHR were orally administered 100 μg/kg LPSp and 0.1% NaCl, and blood pressure was measured at 6, 10, 13 and 16 weeks.					
31366552	0	13	theme	Salt	109:112	arg1	Loading					114:120	Salt Loading	109:120	Salt Loading	109:120	Preventive Effect of Oral Administration of Pantoea agglomerans-derived LPS in a Hypertensive Rat Model Upon Salt Loading.					
31366552	4	14	theme	blood	606:610	arg1	pressure					612:619	blood pressure	606:619	blood pressure	606:619	SHR were orally administered 100 μg/kg LPSp and 0.1% NaCl, and blood pressure was measured at 6, 10, 13 and 16 weeks.					
31366552	1	15	theme	lipopolysaccharide	189:206	arg1	administration					143:156	BACKGROUND/AIM Oral administration	123:156	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp)	123:213	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	2	16	theme	reserve	393:399	arg1	arm					401:403	reserve arm	393:403	reserve arm	393:403	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	5	17	theme	age	689:691	arg1	weeks					680:684	16 weeks	677:684	16 weeks of age	677:691	Furthermore, at 16 weeks of age, blood biochemical markers were measured and microbial community composition was analyzed.					
31366552	3	18	theme	MATERIALS	437:445	arg1	SHR					489:491	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR) and WKY rat were bred from 6 to 16 weeks of age.					
31366552	0	19	theme	Preventive	0:9	arg1	Effect					11:16	Preventive Effect	0:16	Preventive Effect of Oral Administration of Pantoea	0:50	Preventive Effect of Oral Administration of Pantoea agglomerans-derived LPS in a Hypertensive Rat Model Upon Salt Loading.					
31366552	2	20	theme	preventive	332:341	arg1	effect					343:348	the preventive effect	328:348	the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases	328:434	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	7	21	from	pressure	917:924	arg1	SHRs					929:932	SHRs	929:932	SHRs that received LPSp	929:951	Although there was almost no reduction in blood pressure in SHRs that received LPSp.					
31366552	0	22	theme	Oral	21:24	arg1	Administration					26:39	Oral Administration	21:39	Oral Administration of Pantoea	21:50	Preventive Effect of Oral Administration of Pantoea agglomerans-derived LPS in a Hypertensive Rat Model Upon Salt Loading.					
31366552	9	23	theme	LPSp	1056:1059	arg1	administration					1038:1051	CONCLUSION Oral administration	1022:1051	CONCLUSION Oral administration of LPSp	1022:1059	CONCLUSION Oral administration of LPSp showed a preventive effect on salt-loaded hypertension.					
31366552	8	24	theme	age	990:992	arg1	weeks					981:985	13-16 weeks	975:985	13-16 weeks of age	975:992	It was suppressed at 13-16 weeks of age in those with salt loading.					
31366552	3	25	theme	age	538:540	arg1	weeks					529:533	6 to 16 weeks	521:533	6 to 16 weeks of age	521:540	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR) and WKY rat were bred from 6 to 16 weeks of age.					
31366552	5	26	theme	community	748:756	arg1	composition					758:768	microbial community composition	738:768	microbial community composition	738:768	Furthermore, at 16 weeks of age, blood biochemical markers were measured and microbial community composition was analyzed.					
31366552	5	27	theme	microbial	738:746	arg1	composition					758:768	microbial community composition	738:768	microbial community composition	738:768	Furthermore, at 16 weeks of age, blood biochemical markers were measured and microbial community composition was analyzed.					
31366552	1	28	link	agglomerans-derived	169:187	arg1	LPSp					209:212	LPSp	209:212	LPSp	209:212	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	1	28	link	agglomerans-derived	169:187	arg1	lipopolysaccharide					189:206	Pantoea agglomerans-derived lipopolysaccharide	161:206	Pantoea agglomerans-derived lipopolysaccharide (LPSp)	161:213	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	3	29	dep	16	526:527	arg1	to					523:524	to	523:524	to	523:524	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR) and WKY rat were bred from 6 to 16 weeks of age.					
31366552	3	30	theme	hypertensive	471:482	arg1	SHR					489:491	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR) and WKY rat were bred from 6 to 16 weeks of age.					
31366552	2	31	theme	lifestyle-related	409:425	arg1	diseases					427:434	lifestyle-related diseases	409:434	lifestyle-related diseases	409:434	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	0	32	theme	Administration	26:39	arg1	Effect					11:16	Preventive Effect	0:16	Preventive Effect of Oral Administration of Pantoea	0:50	Preventive Effect of Oral Administration of Pantoea agglomerans-derived LPS in a Hypertensive Rat Model Upon Salt Loading.					
31366552	5	33	theme	blood	694:698	arg1	markers					712:718	blood biochemical markers	694:718	blood biochemical markers	694:718	Furthermore, at 16 weeks of age, blood biochemical markers were measured and microbial community composition was analyzed.					
31366552	6	34	theme	RESULTS	784:790	arg1	SHRs					792:795	RESULTS SHRs	784:795	RESULTS SHRs	784:795	RESULTS SHRs developed hypertension with age, which was exacerbated by salt loading.					
31366552	4	35	theme	%	594:594	arg1	NaCl					596:599	0.1% NaCl	591:599	0.1% NaCl	591:599	SHR were orally administered 100 μg/kg LPSp and 0.1% NaCl, and blood pressure was measured at 6, 10, 13 and 16 weeks.					
31366552	9	36	theme	CONCLUSION	1022:1031	arg1	administration					1038:1051	CONCLUSION Oral administration	1022:1051	CONCLUSION Oral administration of LPSp	1022:1059	CONCLUSION Oral administration of LPSp showed a preventive effect on salt-loaded hypertension.					
31366552	3	37	theme	rat	484:486	arg1	SHR					489:491	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR) and WKY rat were bred from 6 to 16 weeks of age.					
31366552	5	38	theme	biochemical	700:710	arg1	markers					712:718	blood biochemical markers	694:718	blood biochemical markers	694:718	Furthermore, at 16 weeks of age, blood biochemical markers were measured and microbial community composition was analyzed.					
31366552	8	39	theme	salt	1008:1011	arg1	loading					1013:1019	salt loading	1008:1019	salt loading	1008:1019	It was suppressed at 13-16 weeks of age in those with salt loading.					
31366552	3	40	theme	METHODS	451:457	arg1	SHR					489:491	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR) and WKY rat were bred from 6 to 16 weeks of age.					
31366552	9	41	theme	Oral	1033:1036	arg1	administration					1038:1051	CONCLUSION Oral administration	1022:1051	CONCLUSION Oral administration of LPSp	1022:1059	CONCLUSION Oral administration of LPSp showed a preventive effect on salt-loaded hypertension.					
31366552	0	42	theme	Pantoea	44:50	arg1	Administration					26:39	Oral Administration	21:39	Oral Administration of Pantoea	21:50	Preventive Effect of Oral Administration of Pantoea agglomerans-derived LPS in a Hypertensive Rat Model Upon Salt Loading.					
31366552	1	43	contain	have	236:239	arg2	effect					254:259	a preventive effect	241:259	a preventive effect	241:259	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	1	43	contain	have	236:239	arg1	administration					143:156	BACKGROUND/AIM Oral administration	123:156	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp)	123:213	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	2	44	theme	blood	358:362	arg1	kind					385:388	a kind	383:388	a kind of reserve arm for lifestyle-related diseases	383:434	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	2	44	theme	blood	358:362	arg1	pressure					364:371	high blood pressure	353:371	high blood pressure	353:371	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	6	45	theme	salt	855:858	arg1	loading					860:866	salt loading	855:866	salt loading	855:866	RESULTS SHRs developed hypertension with age, which was exacerbated by salt loading.					
31366552	1	46	theme	BACKGROUND/AIM	123:136	arg1	administration					143:156	BACKGROUND/AIM Oral administration	123:156	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp)	123:213	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	7	47	theme	blood	911:915	arg1	pressure					917:924	blood pressure	911:924	blood pressure in SHRs that received LPSp	911:951	Although there was almost no reduction in blood pressure in SHRs that received LPSp.					
31366552	1	48	theme	Oral	138:141	arg1	administration					143:156	BACKGROUND/AIM Oral administration	123:156	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp)	123:213	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	2	49	theme	high	353:356	arg1	kind					385:388	a kind	383:388	a kind of reserve arm for lifestyle-related diseases	383:434	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	2	49	theme	high	353:356	arg1	pressure					364:371	high blood pressure	353:371	high blood pressure	353:371	Therefore, we examined the preventive effect on high blood pressure, which is a kind of reserve arm for lifestyle-related diseases.					
31366552	3	50	theme	Spontaneous	459:469	arg1	SHR					489:491	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR)	437:492	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR) and WKY rat were bred from 6 to 16 weeks of age.					
31366552	1	51	theme	preventive	243:252	arg1	effect					254:259	a preventive effect	241:259	a preventive effect	241:259	BACKGROUND/AIM Oral administration of Pantoea agglomerans-derived lipopolysaccharide (LPSp) has been reported to have a preventive effect against various lifestyle-related diseases.					
31366552	4	52	theme	μg/kg	576:580	arg1	LPSp					582:585	100 μg/kg LPSp	572:585	100 μg/kg LPSp	572:585	SHR were orally administered 100 μg/kg LPSp and 0.1% NaCl, and blood pressure was measured at 6, 10, 13 and 16 weeks.					
31366552	3	53	dep	SHR	489:491	arg1	rat					502:504	rat	502:504	rat	502:504	MATERIALS AND METHODS Spontaneous hypertensive rat (SHR) and WKY rat were bred from 6 to 16 weeks of age.					
31366552	4	54	theme	0.1	591:593	arg1	%					594:594	%	594:594	%	594:594	SHR were orally administered 100 μg/kg LPSp and 0.1% NaCl, and blood pressure was measured at 6, 10, 13 and 16 weeks.					
30902717	7	0	theme	endemic	1179:1185	arg1	area					1187:1190	a fluoride endemic area	1168:1190	a fluoride endemic area of Dindigul district, Tamilnadu, India	1168:1229	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	1	1	theme	sorption	350:357	arg1	studies					359:365	fluoride sorption studies	341:365	fluoride sorption studies in batch mode	341:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	2	from	composite	327:335	arg1	fabrication					241:251	the fabrication	237:251	the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode	237:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	2	3	contain	possesses	524:532	arg1	hydrocalumite					499:511	hydrocalumite	499:511	hydrocalumite (HC) which possesses the DC of 2.4mg/g	499:550	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	2	3	contain	possesses	524:532	arg1	HC					514:515	HC	514:515	HC	514:515	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	2	3	contain	possesses	524:532	arg2	DC					538:539	the DC	534:539	the DC of 2.4mg/g	534:550	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	1	4	theme	oxide	270:274	arg1	particles					284:292	magnetic iron oxide (Fe3O4) particles	256:292	magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode	256:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	0	5	theme	defluoridation	93:106	arg1	studies					108:114	defluoridation studies	93:114	defluoridation studies	93:114	Hydrothermal synthesis of magnetic iron oxide encrusted hydrocalumite-chitosan composite for defluoridation studies.					
30902717	3	6	theme	fluoride	647:654	arg1	concentration					656:668	initial fluoride concentration	639:668	initial fluoride concentration	639:668	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	1	7	theme	Fe3O4	199:203	arg1	hydrocalumite-chitosan					175:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan	117:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite	117:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	7	theme	Fe3O4	199:203	arg1	HCCS					205:208	Fe3O4@HCCS	199:208	Fe3O4@HCCS	199:208	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	4	8	with	SEM	837:839	arg1	techniques					851:860	EDAX techniques	846:860	EDAX techniques	846:860	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR and SEM with EDAX techniques.					
30902717	2	9	theme	6.8mg/g	479:485	arg1	capacity					462:469	an enhanced defluoridation capacity	435:469	an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g	435:550	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	2	9	theme	6.8mg/g	479:485	arg1	DC					472:473	DC	472:473	DC	472:473	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	4	10	theme	sorbent	765:771	arg1	changes					750:756	The structural changes	735:756	The structural changes of the sorbent, before and after fluoride sorption	735:807	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR and SEM with EDAX techniques.					
30902717	1	11	dep	hydrocalumite-chitosan	175:196	arg1	composite					211:219	composite	211:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite	117:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	4	12	theme	structural	739:748	arg1	changes					750:756	The structural changes	735:756	The structural changes of the sorbent, before and after fluoride sorption	735:807	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR and SEM with EDAX techniques.					
30902717	1	13	theme	batch	370:374	arg1	mode					376:379	batch mode	370:379	batch mode	370:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	7	14	theme	India	1225:1229	arg1	area					1187:1190	a fluoride endemic area	1168:1190	a fluoride endemic area of Dindigul district, Tamilnadu, India	1168:1229	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	5	15	theme	Langmuir	919:926	arg1	isotherms					928:936	Langmuir isotherms	919:936	Langmuir isotherms	919:936	The equilibrium data was well modeled by Freundlich and Langmuir isotherms.					
30902717	7	16	theme	composite	1102:1110	arg1	efficiency					1077:1086	efficiency	1077:1086	efficiency	1077:1086	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	7	16	theme	composite	1102:1110	arg1	performance					1061:1071	field performance	1055:1071	field performance	1055:1071	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	1	17	theme	@	204:204	arg1	hydrocalumite-chitosan					175:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan	117:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite	117:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	17	theme	@	204:204	arg1	HCCS					205:208	Fe3O4@HCCS	199:208	Fe3O4@HCCS	199:208	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	7	18	dep	performance	1061:1071	arg1	The					1051:1053	The	1051:1053	The	1051:1053	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	3	19	theme	maximum	709:715	arg1	removal					726:732	the maximum fluoride removal	705:732	the maximum fluoride removal	705:732	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	1	20	from	studies	359:365	arg1	mode					376:379	batch mode	370:379	batch mode	370:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	2	21	theme	Fe3O4	404:408	arg1	composite					415:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	3	22	theme	fluoride	717:724	arg1	removal					726:732	the maximum fluoride removal	705:732	the maximum fluoride removal	705:732	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	1	23	from	particles	284:292	arg1	composite					327:335	hydrocalumite-chitosan (HCCS) composite	297:335	hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode	297:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	3	24	theme	various	557:563	arg1	anions					631:636	co-existing anions	619:636	co-existing anions	619:636	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	24	theme	various	557:563	arg1	time					609:612	contact time	601:612	contact time	601:612	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	24	theme	various	557:563	arg1	pH					615:616	pH	615:616	pH	615:616	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	24	theme	various	557:563	arg1	concentration					656:668	initial fluoride concentration	639:668	initial fluoride concentration	639:668	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	24	theme	various	557:563	arg1	parameters					582:591	The various physico-chemical parameters	553:591	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature	553:684	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	24	theme	various	557:563	arg1	temperature					674:684	temperature	674:684	temperature	674:684	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	2	25	theme	magnetic	395:402	arg1	composite					415:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	0	26	theme	Hydrothermal	0:11	arg1	synthesis					13:21	Hydrothermal synthesis	0:21	Hydrothermal synthesis of magnetic iron oxide	0:44	Hydrothermal synthesis of magnetic iron oxide encrusted hydrocalumite-chitosan composite for defluoridation studies.					
30902717	1	27	theme	particles	284:292	arg1	fabrication					241:251	the fabrication	237:251	the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode	237:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	7	28	theme	HCCS	1097:1100	arg1	composite					1102:1110	Fe3O4@HCCS composite	1091:1110	Fe3O4@HCCS composite	1091:1110	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	3	29	theme	physico-chemical	565:580	arg1	anions					631:636	co-existing anions	619:636	co-existing anions	619:636	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	29	theme	physico-chemical	565:580	arg1	time					609:612	contact time	601:612	contact time	601:612	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	29	theme	physico-chemical	565:580	arg1	pH					615:616	pH	615:616	pH	615:616	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	29	theme	physico-chemical	565:580	arg1	concentration					656:668	initial fluoride concentration	639:668	initial fluoride concentration	639:668	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	29	theme	physico-chemical	565:580	arg1	parameters					582:591	The various physico-chemical parameters	553:591	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature	553:684	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	3	29	theme	physico-chemical	565:580	arg1	temperature					674:684	temperature	674:684	temperature	674:684	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	6	30	theme	endothermic	1010:1020	arg1	nature					1022:1027	endothermic nature	1010:1027	endothermic nature	1010:1027	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.					
30902717	2	31	theme	prepared	386:393	arg1	composite					415:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	0	32	theme	magnetic	26:33	arg1	oxide					40:44	magnetic iron oxide	26:44	magnetic iron oxide	26:44	Hydrothermal synthesis of magnetic iron oxide encrusted hydrocalumite-chitosan composite for defluoridation studies.					
30902717	2	33	theme	2.4mg/g	544:550	arg1	DC					538:539	the DC	534:539	the DC of 2.4mg/g	534:550	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	1	34	theme	magnetic	119:126	arg1	hydrocalumite-chitosan					175:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan	117:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite	117:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	34	theme	magnetic	119:126	arg1	HCCS					205:208	Fe3O4@HCCS	199:208	Fe3O4@HCCS	199:208	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	2	35	theme	enhanced	438:445	arg1	capacity					462:469	an enhanced defluoridation capacity	435:469	an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g	435:550	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	2	35	theme	enhanced	438:445	arg1	DC					472:473	DC	472:473	DC	472:473	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	1	36	theme	hydrocalumite-chitosan	297:318	arg1	composite					327:335	hydrocalumite-chitosan (HCCS) composite	297:335	hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode	297:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	7	37	theme	Fe3O4	1091:1095	arg1	composite					1102:1110	Fe3O4@HCCS composite	1091:1110	Fe3O4@HCCS composite	1091:1110	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	1	38	theme	adsorbent	128:136	arg1	hydrocalumite-chitosan					175:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan	117:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite	117:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	38	theme	adsorbent	128:136	arg1	HCCS					205:208	Fe3O4@HCCS	199:208	Fe3O4@HCCS	199:208	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	4	39	theme	EDAX	846:849	arg1	techniques					851:860	EDAX techniques	846:860	EDAX techniques	846:860	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR and SEM with EDAX techniques.					
30902717	0	40	theme	oxide	40:44	arg1	synthesis					13:21	Hydrothermal synthesis	0:21	Hydrothermal synthesis of magnetic iron oxide	0:44	Hydrothermal synthesis of magnetic iron oxide encrusted hydrocalumite-chitosan composite for defluoridation studies.					
30902717	3	41	theme	initial	639:645	arg1	concentration					656:668	initial fluoride concentration	639:668	initial fluoride concentration	639:668	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	5	42	theme	equilibrium	867:877	arg1	data					879:882	The equilibrium data	863:882	The equilibrium data	863:882	The equilibrium data was well modeled by Freundlich and Langmuir isotherms.					
30902717	0	43	theme	iron	35:38	arg1	oxide					40:44	magnetic iron oxide	26:44	magnetic iron oxide	26:44	Hydrothermal synthesis of magnetic iron oxide encrusted hydrocalumite-chitosan composite for defluoridation studies.					
30902717	1	44	theme	HCCS	321:324	arg1	composite					327:335	hydrocalumite-chitosan (HCCS) composite	297:335	hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode	297:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	3	45	theme	co-existing	619:629	arg1	anions					631:636	co-existing anions	619:636	co-existing anions	619:636	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	2	46	theme	defluoridation	447:460	arg1	capacity					462:469	an enhanced defluoridation capacity	435:469	an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g	435:550	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	2	46	theme	defluoridation	447:460	arg1	DC					472:473	DC	472:473	DC	472:473	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	2	47	theme	HCCS	410:413	arg1	composite					415:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	0	48	theme	hydrocalumite-chitosan	56:77	arg1	composite					79:87	hydrocalumite-chitosan composite	56:87	hydrocalumite-chitosan composite	56:87	Hydrothermal synthesis of magnetic iron oxide encrusted hydrocalumite-chitosan composite for defluoridation studies.					
30902717	6	49	theme	thermodynamic	943:955	arg1	parameters					957:966	The thermodynamic parameters	939:966	The thermodynamic parameters	939:966	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.					
30902717	7	50	theme	field	1055:1059	arg1	performance					1061:1071	field performance	1055:1071	field performance	1055:1071	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	7	51	theme	@	1096:1096	arg1	composite					1102:1110	Fe3O4@HCCS composite	1091:1110	Fe3O4@HCCS composite	1091:1110	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	2	52	theme	@	409:409	arg1	composite					415:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	7	53	theme	waste-water	1134:1144	arg1	sample					1146:1151	the waste-water sample	1130:1151	the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols	1130:1254	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	1	54	theme	magnetic	145:152	arg1	hydrocalumite-chitosan					175:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan	117:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite	117:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	54	theme	magnetic	145:152	arg1	HCCS					205:208	Fe3O4@HCCS	199:208	Fe3O4@HCCS	199:208	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	7	55	theme	fluoride	1170:1177	arg1	area					1187:1190	a fluoride endemic area	1168:1190	a fluoride endemic area of Dindigul district, Tamilnadu, India	1168:1229	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	1	56	from	fabrication	241:251	arg1	composite					327:335	hydrocalumite-chitosan (HCCS) composite	297:335	hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode	297:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	3	57	theme	contact	601:607	arg1	time					609:612	contact time	601:612	contact time	601:612	The various physico-chemical parameters such as contact time, pH, co-existing anions, initial fluoride concentration and temperature were optimized for the maximum fluoride removal.					
30902717	1	58	theme	iron	154:157	arg1	hydrocalumite-chitosan					175:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan	117:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite	117:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	58	theme	iron	154:157	arg1	HCCS					205:208	Fe3O4@HCCS	199:208	Fe3O4@HCCS	199:208	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	4	59	with	FTIR	828:831	arg1	techniques					851:860	EDAX techniques	846:860	EDAX techniques	846:860	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR and SEM with EDAX techniques.					
30902717	2	60	contain	possesses	425:433	arg1	composite					415:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite	382:423	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	2	60	contain	possesses	425:433	arg2	DC					472:473	DC	472:473	DC	472:473	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	2	60	contain	possesses	425:433	arg2	capacity					462:469	an enhanced defluoridation capacity	435:469	an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g	435:550	The prepared magnetic Fe3O4@HCCS composite possesses an enhanced defluoridation capacity (DC) of 6.8mg/g compared to hydrocalumite (HC) which possesses the DC of 2.4mg/g.					
30902717	1	61	theme	oxide	159:163	arg1	hydrocalumite-chitosan					175:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan	117:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite	117:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	61	theme	oxide	159:163	arg1	HCCS					205:208	Fe3O4@HCCS	199:208	Fe3O4@HCCS	199:208	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	6	62	theme	fluoride	1032:1039	arg1	sorption					1041:1048	fluoride sorption	1032:1048	fluoride sorption	1032:1048	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.					
30902717	1	63	theme	encrusted	165:173	arg1	hydrocalumite-chitosan					175:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan	117:196	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite	117:219	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	63	theme	encrusted	165:173	arg1	HCCS					205:208	Fe3O4@HCCS	199:208	Fe3O4@HCCS	199:208	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	64	theme	magnetic	256:263	arg1	Fe3O4					277:281	Fe3O4	277:281	Fe3O4	277:281	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	64	theme	magnetic	256:263	arg1	oxide					270:274	magnetic iron oxide	256:274	magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode	256:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	7	65	theme	standard	1237:1244	arg1	protocols					1246:1254	standard protocols	1237:1254	standard protocols	1237:1254	The field performance and efficiency of Fe3O4@HCCS composite was examined with the waste-water sample collected from a fluoride endemic area of Dindigul district, Tamilnadu, India using standard protocols.					
30902717	1	66	theme	fluoride	341:348	arg1	studies					359:365	fluoride sorption studies	341:365	fluoride sorption studies in batch mode	341:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	4	67	theme	fluoride	791:798	arg1	sorption					800:807	fluoride sorption	791:807	fluoride sorption	791:807	The structural changes of the sorbent, before and after fluoride sorption were studied using FTIR and SEM with EDAX techniques.					
30902717	6	68	theme	sorption	1041:1048	arg1	spontaneity					994:1004	spontaneity	994:1004	spontaneity	994:1004	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.					
30902717	6	68	theme	sorption	1041:1048	arg1	nature					1022:1027	endothermic nature	1010:1027	endothermic nature	1010:1027	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.					
30902717	6	68	theme	sorption	1041:1048	arg1	feasibility					981:991	feasibility	981:991	feasibility	981:991	The thermodynamic parameters revealed the feasibility, spontaneity and endothermic nature of fluoride sorption.					
30902717	1	69	theme	iron	265:268	arg1	Fe3O4					277:281	Fe3O4	277:281	Fe3O4	277:281	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
30902717	1	69	theme	iron	265:268	arg1	oxide					270:274	magnetic iron oxide	256:274	magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode	256:379	A magnetic adsorbent namely magnetic iron oxide encrusted hydrocalumite-chitosan (Fe3O4@HCCS) composite was prepared by the fabrication of magnetic iron oxide (Fe3O4) particles on hydrocalumite-chitosan (HCCS) composite for fluoride sorption studies in batch mode.					
31546343	1	0	theme	body	167:170	arg1	FBR					182:184	FBR	182:184	FBR	182:184	Current bone implants based on new biomaterials may cause a foreign body reaction (FBR) around the implant itself thus prolonging the healing time following bone fractures.					
31546343	1	0	theme	body	167:170	arg1	reaction					172:179	a foreign body reaction	157:179	a foreign body reaction (FBR)	157:185	Current bone implants based on new biomaterials may cause a foreign body reaction (FBR) around the implant itself thus prolonging the healing time following bone fractures.					
31546343	4	1	theme	co-culture	771:780	arg1	model					782:786	in vitro co-culture model	762:786	in vitro co-culture model based on osteoblasts and macrophages stimulated by LPS	762:841	Then, to mimic the inflammation process at osteoporotic site, the effect of scaffolds was evaluated on in vitro co-culture model based on osteoblasts and macrophages stimulated by LPS.					
31546343	5	2	from	generation	1051:1060	arg1	hMSCs					1073:1077	hMSCs	1073:1077	hMSCs	1073:1077	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	3	3	from	potential	439:447	arg1	hMSCs					465:469	hMSCs	465:469	hMSCs stimulated by lipopolysaccharide (LPS)	465:508	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	4	4	theme	osteoporotic	702:713	arg1	site					715:718	osteoporotic site	702:718	osteoporotic site	702:718	Then, to mimic the inflammation process at osteoporotic site, the effect of scaffolds was evaluated on in vitro co-culture model based on osteoblasts and macrophages stimulated by LPS.					
31546343	1	5	theme	bone	256:259	arg1	fractures					261:269	bone fractures	256:269	bone fractures	256:269	Current bone implants based on new biomaterials may cause a foreign body reaction (FBR) around the implant itself thus prolonging the healing time following bone fractures.					
31546343	3	6	dep	metabolites	590:600	arg1	metabolites					590:600	oxidative stress metabolites	573:600	oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response	573:656	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	6	dep	metabolites	590:600	arg1	nitrites					620:627	nitrites	620:627	nitrites	620:627	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	6	dep	metabolites	590:600	arg1	IL-10					610:614	IL-10	610:614	IL-10	610:614	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	6	dep	metabolites	590:600	arg1	IL-1β					603:607	IL-1β	603:607	IL-1β	603:607	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	7	dep	dosing	530:535	arg1	levels					541:546	the levels	537:546	the levels of some interleukins	537:567	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	4	8	theme	in	762:763	arg1	model					782:786	in vitro co-culture model	762:786	in vitro co-culture model based on osteoblasts and macrophages stimulated by LPS	762:841	Then, to mimic the inflammation process at osteoporotic site, the effect of scaffolds was evaluated on in vitro co-culture model based on osteoblasts and macrophages stimulated by LPS.					
31546343	3	9	theme	oxidative	573:581	arg1	metabolites					590:600	oxidative stress metabolites	573:600	oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response	573:656	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	9	theme	oxidative	573:581	arg1	nitrites					620:627	nitrites	620:627	nitrites	620:627	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	9	theme	oxidative	573:581	arg1	IL-10					610:614	IL-10	610:614	IL-10	610:614	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	9	theme	oxidative	573:581	arg1	IL-1β					603:607	IL-1β	603:607	IL-1β	603:607	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	2	10	dep	scaffolds	313:321	arg1	controlling					362:372	controlling	362:372	controlling inflammatory response	362:394	In this paper, biomimetic chitosan-based scaffolds promoting bone tissue regeneration and controlling inflammatory response are proposed.					
31546343	2	10	dep	scaffolds	313:321	arg1	promoting					323:331	promoting	323:331	promoting bone tissue regeneration	323:356	In this paper, biomimetic chitosan-based scaffolds promoting bone tissue regeneration and controlling inflammatory response are proposed.					
31546343	5	11	dep	reduce	971:976	arg1	ii					967:968	ii	967:968	ii	967:968	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	4	12	theme	inflammation	678:689	arg1	process					691:697	the inflammation process	674:697	the inflammation process	674:697	Then, to mimic the inflammation process at osteoporotic site, the effect of scaffolds was evaluated on in vitro co-culture model based on osteoblasts and macrophages stimulated by LPS.					
31546343	5	13	theme	bioactivated	870:881	arg1	scaffolds					883:891	bioactivated scaffolds	870:891	bioactivated scaffolds	870:891	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	3	14	theme	anti-inflammatory	421:437	arg1	potential					439:447	the anti-inflammatory potential	417:447	the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS)	417:508	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	1	15	theme	Current	99:105	arg1	implants					112:119	Current bone implants	99:119	Current bone implants based on new biomaterials	99:145	Current bone implants based on new biomaterials may cause a foreign body reaction (FBR) around the implant itself thus prolonging the healing time following bone fractures.					
31546343	1	16	theme	bone	107:110	arg1	implants					112:119	Current bone implants	99:119	Current bone implants based on new biomaterials	99:145	Current bone implants based on new biomaterials may cause a foreign body reaction (FBR) around the implant itself thus prolonging the healing time following bone fractures.					
31546343	3	17	theme	scaffolds	452:460	arg1	potential					439:447	the anti-inflammatory potential	417:447	the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS)	417:508	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	0	18	theme	Osteoinductive	0:13	arg1	properties					37:46	Osteoinductive and anti-inflammatory properties	0:46	Osteoinductive and anti-inflammatory properties of chitosan-based scaffolds for bone regeneration	0:96	Osteoinductive and anti-inflammatory properties of chitosan-based scaffolds for bone regeneration.					
31546343	4	19	theme	scaffolds	735:743	arg1	effect					725:730	the effect	721:730	the effect of scaffolds	721:743	Then, to mimic the inflammation process at osteoporotic site, the effect of scaffolds was evaluated on in vitro co-culture model based on osteoblasts and macrophages stimulated by LPS.					
31546343	5	20	theme	oxidative	978:986	arg1	metabolites					995:1005	oxidative stress metabolites	978:1005	oxidative stress metabolites	978:1005	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	5	21	theme	mediators	942:950	arg1	synthesis					916:924	synthesis	916:924	synthesis of inflammatory mediators such as IL-1β	916:964	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	3	22	theme	stress	583:588	arg1	metabolites					590:600	oxidative stress metabolites	573:600	oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response	573:656	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	22	theme	stress	583:588	arg1	nitrites					620:627	nitrites	620:627	nitrites	620:627	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	22	theme	stress	583:588	arg1	IL-10					610:614	IL-10	610:614	IL-10	610:614	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	22	theme	stress	583:588	arg1	IL-1β					603:607	IL-1β	603:607	IL-1β	603:607	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	0	23	theme	anti-inflammatory	19:35	arg1	properties					37:46	Osteoinductive and anti-inflammatory properties	0:46	Osteoinductive and anti-inflammatory properties of chitosan-based scaffolds for bone regeneration	0:96	Osteoinductive and anti-inflammatory properties of chitosan-based scaffolds for bone regeneration.					
31546343	2	24	theme	biomimetic	287:296	arg1	scaffolds					313:321	biomimetic chitosan-based scaffolds	287:321	biomimetic chitosan-based scaffolds promoting bone tissue regeneration and controlling inflammatory response	287:394	In this paper, biomimetic chitosan-based scaffolds promoting bone tissue regeneration and controlling inflammatory response are proposed.					
31546343	6	25	theme	in	1155:1156	arg1	co-cultures					1164:1174	in vitro co-cultures	1155:1174	in vitro co-cultures	1155:1174	Finally, bioactivated scaffolds show an anti-inflammatory activity also on in vitro co-cultures, which better mimic in vivo damaged bone microenvironment.					
31546343	0	26	theme	chitosan-based	51:64	arg1	scaffolds					66:74	chitosan-based scaffolds	51:74	chitosan-based scaffolds for bone regeneration	51:96	Osteoinductive and anti-inflammatory properties of chitosan-based scaffolds for bone regeneration.					
31546343	6	27	dep	mimic	1190:1194	arg1	damaged					1204:1210	damaged	1204:1210	mimic in vivo damaged bone microenvironment	1190:1232	Finally, bioactivated scaffolds show an anti-inflammatory activity also on in vitro co-cultures, which better mimic in vivo damaged bone microenvironment.					
31546343	6	28	dep	in	1155:1156	arg1	vitro					1158:1162	vitro	1158:1162	vitro	1158:1162	Finally, bioactivated scaffolds show an anti-inflammatory activity also on in vitro co-cultures, which better mimic in vivo damaged bone microenvironment.					
31546343	5	29	dep	promote	1017:1023	arg1	iii					1012:1014	iii	1012:1014	iii	1012:1014	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	5	30	dep	inhibit	908:914	arg1	i					905:905	i	905:905	i	905:905	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	1	31	theme	new	130:132	arg1	biomaterials					134:145	new biomaterials	130:145	new biomaterials	130:145	Current bone implants based on new biomaterials may cause a foreign body reaction (FBR) around the implant itself thus prolonging the healing time following bone fractures.					
31546343	0	32	theme	scaffolds	66:74	arg1	properties					37:46	Osteoinductive and anti-inflammatory properties	0:46	Osteoinductive and anti-inflammatory properties of chitosan-based scaffolds for bone regeneration	0:96	Osteoinductive and anti-inflammatory properties of chitosan-based scaffolds for bone regeneration.					
31546343	5	33	theme	markers	1043:1049	arg1	IL-10					1063:1067	IL-10	1063:1067	IL-10	1063:1067	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	5	33	theme	markers	1043:1049	arg1	generation					1051:1060	anti-inflammatory markers generation	1025:1060	anti-inflammatory markers generation (IL-10) in hMSCs	1025:1077	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	5	34	theme	anti-inflammatory	1025:1041	arg1	markers					1043:1049	anti-inflammatory markers	1025:1049	anti-inflammatory markers generation (IL-10) in hMSCs	1025:1077	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	6	35	from	activity	1138:1145	arg1	co-cultures					1164:1174	in vitro co-cultures	1155:1174	in vitro co-cultures	1155:1174	Finally, bioactivated scaffolds show an anti-inflammatory activity also on in vitro co-cultures, which better mimic in vivo damaged bone microenvironment.					
31546343	6	36	theme	anti-inflammatory	1120:1136	arg1	activity					1138:1145	an anti-inflammatory activity	1117:1145	an anti-inflammatory activity	1117:1145	Finally, bioactivated scaffolds show an anti-inflammatory activity also on in vitro co-cultures, which better mimic in vivo damaged bone microenvironment.					
31546343	6	37	theme	bone	1212:1215	arg1	microenvironment					1217:1232	bone microenvironment	1212:1232	bone microenvironment	1212:1232	Finally, bioactivated scaffolds show an anti-inflammatory activity also on in vitro co-cultures, which better mimic in vivo damaged bone microenvironment.					
31546343	4	38	dep	in	762:763	arg1	vitro					765:769	vitro	765:769	vitro	765:769	Then, to mimic the inflammation process at osteoporotic site, the effect of scaffolds was evaluated on in vitro co-culture model based on osteoblasts and macrophages stimulated by LPS.					
31546343	5	39	theme	stress	988:993	arg1	metabolites					995:1005	oxidative stress metabolites	978:1005	oxidative stress metabolites	978:1005	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	2	40	theme	tissue	338:343	arg1	regeneration					345:356	bone tissue regeneration	333:356	bone tissue regeneration	333:356	In this paper, biomimetic chitosan-based scaffolds promoting bone tissue regeneration and controlling inflammatory response are proposed.					
31546343	2	41	theme	chitosan-based	298:311	arg1	scaffolds					313:321	biomimetic chitosan-based scaffolds	287:321	biomimetic chitosan-based scaffolds promoting bone tissue regeneration and controlling inflammatory response	287:394	In this paper, biomimetic chitosan-based scaffolds promoting bone tissue regeneration and controlling inflammatory response are proposed.					
31546343	3	42	theme	interleukins	556:567	arg1	levels					541:546	the levels	537:546	the levels of some interleukins	537:567	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	43	theme	immune	642:647	arg1	response					649:656	immune response	642:656	immune response	642:656	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	3	44	from	scaffolds	452:460	arg1	hMSCs					465:469	hMSCs	465:469	hMSCs stimulated by lipopolysaccharide (LPS)	465:508	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	2	45	theme	bone	333:336	arg1	regeneration					345:356	bone tissue regeneration	333:356	bone tissue regeneration	333:356	In this paper, biomimetic chitosan-based scaffolds promoting bone tissue regeneration and controlling inflammatory response are proposed.					
31546343	5	46	theme	inflammatory	929:940	arg1	IL-1β					960:964	IL-1β	960:964	IL-1β	960:964	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	5	46	theme	inflammatory	929:940	arg1	mediators					942:950	inflammatory mediators	929:950	inflammatory mediators such as IL-1β	929:964	Results demonstrated that bioactivated scaffolds are able to i) inhibit synthesis of inflammatory mediators such as IL-1β; ii) reduce oxidative stress metabolites; and iii) promote anti-inflammatory markers generation (IL-10) in hMSCs.					
31546343	6	47	theme	bioactivated	1089:1100	arg1	scaffolds					1102:1110	bioactivated scaffolds	1089:1110	bioactivated scaffolds	1089:1110	Finally, bioactivated scaffolds show an anti-inflammatory activity also on in vitro co-cultures, which better mimic in vivo damaged bone microenvironment.					
31546343	2	48	theme	inflammatory	374:385	arg1	response					387:394	inflammatory response	374:394	inflammatory response	374:394	In this paper, biomimetic chitosan-based scaffolds promoting bone tissue regeneration and controlling inflammatory response are proposed.					
31546343	1	49	theme	healing	233:239	arg1	time					241:244	the healing time	229:244	the healing time following bone fractures	229:269	Current bone implants based on new biomaterials may cause a foreign body reaction (FBR) around the implant itself thus prolonging the healing time following bone fractures.					
31546343	0	50	theme	bone	80:83	arg1	regeneration					85:96	bone regeneration	80:96	bone regeneration	80:96	Osteoinductive and anti-inflammatory properties of chitosan-based scaffolds for bone regeneration.					
31546343	3	51	from	hMSCs	465:469	arg1	potential					439:447	the anti-inflammatory potential	417:447	the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS)	417:508	First, the anti-inflammatory potential of scaffolds on hMSCs stimulated by lipopolysaccharide (LPS) was investigated by dosing the levels of some interleukins and oxidative stress metabolites (IL-1β, IL-10 and nitrites) involved in immune response.					
31546343	1	52	theme	foreign	159:165	arg1	FBR					182:184	FBR	182:184	FBR	182:184	Current bone implants based on new biomaterials may cause a foreign body reaction (FBR) around the implant itself thus prolonging the healing time following bone fractures.					
31546343	1	52	theme	foreign	159:165	arg1	reaction					172:179	a foreign body reaction	157:179	a foreign body reaction (FBR)	157:185	Current bone implants based on new biomaterials may cause a foreign body reaction (FBR) around the implant itself thus prolonging the healing time following bone fractures.					
30794378	7	0	theme	N-glycosylation	1099:1113	arg1	sites					1115:1119	83 N-glycosylation sites	1096:1119	83 N-glycosylation sites	1096:1119	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	3	1	theme	modified	527:534	arg1	phase					536:540	a new modified phase	521:540	a new modified phase of hydrophilic materials in glycoproteomics	521:584	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	3	1	theme	modified	527:534	arg1	Carrageenan					428:438	Carrageenan	428:438	Carrageenan	428:438	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	6	2	theme	bovine	964:969	arg1	BSA					986:988	BSA	986:988	BSA	986:988	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	6	2	theme	bovine	964:969	arg1	albumin					977:983	bovine serum albumin	964:983	bovine serum albumin (BSA) tryptic digest	964:1004	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	9	3	theme	glycopeptides	1647:1659	arg1	enrichment					1661:1670	glycopeptides enrichment	1647:1670	glycopeptides enrichment	1647:1670	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	4	4	gly	glycosylated	713:724	arg1	peptides					726:733	glycosylated peptides	713:733	glycosylated peptides	713:733	The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides.					
30794378	3	5	theme	materials	557:565	arg1	phase					536:540	a new modified phase	521:540	a new modified phase of hydrophilic materials in glycoproteomics	521:584	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	3	5	theme	materials	557:565	arg1	Carrageenan					428:438	Carrageenan	428:438	Carrageenan	428:438	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	5	6	theme	improved	855:862	arg1	ratio					886:890	greatly improved signal-to-noise (S/N) ratio	847:890	greatly improved signal-to-noise (S/N) ratio	847:890	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	6	7	theme	IgG	956:958	arg1	ratio					947:951	the molar ratio	937:951	the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures	937:1013	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	8	8	theme	glycopeptides	1459:1471	arg1	enrichment					1445:1454	enrichment	1445:1454	enrichment of glycopeptides for 20 times	1445:1484	In addition, GO-PEI-Carr exhibited a unique repeatability and stability even after enrichment of glycopeptides for 20 times.					
30794378	4	9	theme	perfect	653:659	arg1	charge					669:674	a perfect surface charge	651:674	the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides	628:733	The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides.					
30794378	7	10	with	glycoproteins	1193:1205	arg1	sites					1232:1236	157 N-glycosylation sites	1212:1236	157 N-glycosylation sites	1212:1236	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	4	11	theme	glycosylated	713:724	arg1	peptides					726:733	glycosylated peptides	713:733	glycosylated peptides	713:733	The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides.					
30794378	4	12	dep	composite	632:640	arg1	the					628:630	the	628:630	the	628:630	The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides.					
30794378	9	13	theme	higher	1507:1512	arg1	sensitivity					1514:1524	a higher sensitivity	1505:1524	a higher sensitivity (1 fmol/μL IgG)	1505:1540	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	9	13	theme	higher	1507:1512	arg1	IgG					1537:1539	1 fmol/μL IgG	1527:1539	1 fmol/μL IgG	1527:1539	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	6	14	dep	IgG	956:958	arg1	mixtures					1006:1013	mixtures	1006:1013	mixtures	1006:1013	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	3	15	from	materials	557:565	arg1	glycoproteomics					570:584	glycoproteomics	570:584	glycoproteomics	570:584	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	1	16	theme	easy	152:155	arg1	route					157:161	an easy route	149:161	an easy route to improve glycopeptides enrichment efficiency	149:208	A hydrophilic nanocomposite was synthesized by an easy route to improve glycopeptides enrichment efficiency.					
30794378	4	17	theme	greater	687:693	arg1	ability					695:701	a greater ability	685:701	the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides	628:733	The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides.					
30794378	3	18	from	glycoproteomics	570:584	arg1	phase					536:540	a new modified phase	521:540	a new modified phase of hydrophilic materials in glycoproteomics	521:584	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	3	18	from	glycoproteomics	570:584	arg1	Carrageenan					428:438	Carrageenan	428:438	Carrageenan	428:438	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	5	19	theme	immunoglobulin	779:792	arg1	IgG					797:799	IgG	797:799	IgG	797:799	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	5	19	theme	immunoglobulin	779:792	arg1	G					794:794	human serum immunoglobulin G	767:794	human serum immunoglobulin G (IgG) tryptic digests	767:816	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	6	20	theme	tryptic	991:997	arg1	digest					999:1004	bovine serum albumin (BSA) tryptic digest	964:1004	bovine serum albumin (BSA) tryptic digest	964:1004	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	0	21	theme	Glycopeptides	87:99	arg1	Enrichment					73:82	the Specific Enrichment	60:82	the Specific Enrichment of Glycopeptides	60:99	Hydrophilic Nanocomposite Functionalized by Carrageenan for the Specific Enrichment of Glycopeptides.					
30794378	1	22	theme	glycopeptides	174:186	arg1	efficiency					199:208	glycopeptides enrichment efficiency	174:208	glycopeptides enrichment efficiency	174:208	A hydrophilic nanocomposite was synthesized by an easy route to improve glycopeptides enrichment efficiency.					
30794378	9	23	theme	research	1740:1747	arg1	application					1710:1720	the application	1706:1720	the application of glycoproteomic research	1706:1747	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	9	24	theme	better	1545:1550	arg1	mg/g					1584:1587	up to ∼300 mg/g	1573:1587	up to ∼300 mg/g	1573:1587	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	9	24	theme	better	1545:1550	arg1	capacity					1563:1570	a better enrichment capacity	1543:1570	a better enrichment capacity (up to ∼300 mg/g)	1543:1588	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	7	25	from	samples	1353:1359	arg1	glycopeptides					1315:1327	glycopeptides	1315:1327	glycopeptides from complex biological samples	1315:1359	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	6	26	theme	albumin	977:983	arg1	digest					999:1004	bovine serum albumin (BSA) tryptic digest	964:1004	bovine serum albumin (BSA) tryptic digest	964:1004	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	5	27	theme	human	767:771	arg1	IgG					797:799	IgG	797:799	IgG	797:799	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	5	27	theme	human	767:771	arg1	G					794:794	human serum immunoglobulin G	767:794	human serum immunoglobulin G (IgG) tryptic digests	767:816	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	7	28	theme	biological	1342:1351	arg1	samples					1353:1359	complex biological samples	1334:1359	complex biological samples	1334:1359	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	5	29	gly	glycopeptides	748:760	arg1	digests					810:816	human serum immunoglobulin G (IgG) tryptic digests	767:816	human serum immunoglobulin G (IgG) tryptic digests	767:816	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	5	29	gly	glycopeptides	748:760	arg2	glycopeptides					748:760	Thirty-four glycopeptides	736:760	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests	736:816	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	0	30	theme	Hydrophilic	0:10	arg1	Nanocomposite					12:24	Hydrophilic Nanocomposite	0:24	Hydrophilic Nanocomposite	0:24	Hydrophilic Nanocomposite Functionalized by Carrageenan for the Specific Enrichment of Glycopeptides.					
30794378	7	31	gly	glycopeptides	1315:1327	arg1	samples					1353:1359	complex biological samples	1334:1359	complex biological samples	1334:1359	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	7	31	gly	glycopeptides	1315:1327	arg2	glycopeptides					1315:1327	glycopeptides	1315:1327	glycopeptides from complex biological samples	1315:1359	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	1	32	theme	hydrophilic	104:114	arg1	nanocomposite					116:128	A hydrophilic nanocomposite	102:128	A hydrophilic nanocomposite	102:128	A hydrophilic nanocomposite was synthesized by an easy route to improve glycopeptides enrichment efficiency.					
30794378	7	33	gly	N-glycosylation	1216:1230	arg2	sites					1232:1236	157 N-glycosylation sites	1212:1236	157 N-glycosylation sites	1212:1236	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	3	34	theme	hydroxyl	469:476	arg1	groups					478:483	hydroxyl groups	469:483	hydroxyl groups	469:483	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	5	35	theme	S/N	881:883	arg1	ratio					886:890	greatly improved signal-to-noise (S/N) ratio	847:890	greatly improved signal-to-noise (S/N) ratio	847:890	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	2	36	theme	oxide/poly	369:378	arg1	support					394:400	graphene oxide/poly(ethylenimine) support	360:400	graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr)	360:425	This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr).					
30794378	9	37	theme	ideal	1598:1602	arg1	recovery					1615:1622	an ideal enrichment recovery	1595:1622	an ideal enrichment recovery (90.8% and 109.5%)	1595:1641	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	9	38	dep	∼300	1579:1582	arg1	to					1576:1577	to	1576:1577	to	1576:1577	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	4	39	theme	carrageenan	607:617	arg1	introduction					591:602	The introduction	587:602	The introduction of carrageenan	587:617	The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides.					
30794378	2	40	theme	new	216:218	arg1	composite					220:228	This new composite	211:228	This new composite	211:228	This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr).					
30794378	7	41	gly	glycoproteins	1077:1089	arg1	glycoproteins					1077:1089	56 glycoproteins	1074:1089	56 glycoproteins with 83 N-glycosylation sites	1074:1119	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	0	42	theme	Specific	64:71	arg1	Enrichment					73:82	the Specific Enrichment	60:82	the Specific Enrichment of Glycopeptides	60:99	Hydrophilic Nanocomposite Functionalized by Carrageenan for the Specific Enrichment of Glycopeptides.					
30794378	7	43	theme	liver	1265:1269	arg1	tissues					1271:1277	mouse liver tissues	1259:1277	mouse liver tissues	1259:1277	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	7	44	theme	157	1212:1214	arg1	sites					1232:1236	157 N-glycosylation sites	1212:1236	157 N-glycosylation sites	1212:1236	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	2	45	theme	ethylenimine	380:391	arg1	support					394:400	graphene oxide/poly(ethylenimine) support	360:400	graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr)	360:425	This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr).					
30794378	3	46	theme	new	523:525	arg1	phase					536:540	a new modified phase	521:540	a new modified phase of hydrophilic materials in glycoproteomics	521:584	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	3	46	theme	new	523:525	arg1	Carrageenan					428:438	Carrageenan	428:438	Carrageenan	428:438	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	9	47	gly	glycopeptides	1647:1659	arg2	glycopeptides					1647:1659	glycopeptides enrichment	1647:1670	glycopeptides enrichment	1647:1670	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	1	48	theme	enrichment	188:197	arg1	efficiency					199:208	glycopeptides enrichment efficiency	174:208	glycopeptides enrichment efficiency	174:208	A hydrophilic nanocomposite was synthesized by an easy route to improve glycopeptides enrichment efficiency.					
30794378	6	49	theme	good	895:898	arg1	selectivity					900:910	A good selectivity	893:910	A good selectivity	893:910	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	4	50	theme	surface	661:667	arg1	charge					669:674	a perfect surface charge	651:674	the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides	628:733	The introduction of carrageenan provided the composite not only a perfect surface charge but also a greater ability to enrich glycosylated peptides.					
30794378	7	51	theme	N-glycosylation	1216:1230	arg1	sites					1232:1236	157 N-glycosylation sites	1212:1236	157 N-glycosylation sites	1212:1236	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	6	52	theme	serum	971:975	arg1	BSA					986:988	BSA	986:988	BSA	986:988	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	6	52	theme	serum	971:975	arg1	albumin					977:983	bovine serum albumin	964:983	bovine serum albumin (BSA) tryptic digest	964:1004	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	7	53	attach	derived	1061:1067	arg1	glycoproteins					1077:1089	56 glycoproteins	1074:1089	56 glycoproteins with 83 N-glycosylation sites	1074:1119	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	7	53	attach	derived	1061:1067	arg2	glycopeptides					1047:1059	76 glycopeptides	1044:1059	76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites	1044:1119	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	7	53	attach	derived	1061:1067	arg2	Meanwhile					1033:1041	Meanwhile	1033:1041	Meanwhile	1033:1041	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	3	54	theme	hydrophilic	545:555	arg1	materials					557:565	hydrophilic materials	545:565	hydrophilic materials in glycoproteomics	545:584	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	9	55	dep	recovery	1615:1622	arg1	%					1629:1629	90.8%	1625:1629	90.8%	1625:1629	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	9	55	dep	recovery	1615:1622	arg1	%					1640:1640	109.5%	1635:1640	109.5%	1635:1640	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	5	56	theme	signal-to-noise	864:878	arg1	ratio					886:890	greatly improved signal-to-noise (S/N) ratio	847:890	greatly improved signal-to-noise (S/N) ratio	847:890	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	6	57	theme	molar	941:945	arg1	ratio					947:951	the molar ratio	937:951	the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures	937:1013	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	9	58	theme	great	1686:1690	arg1	potential					1692:1700	a great potential	1684:1700	a great potential for the application of glycoproteomic research	1684:1747	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	3	59	contain	has	447:449	arg1	phase					536:540	a new modified phase	521:540	a new modified phase of hydrophilic materials in glycoproteomics	521:584	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	3	59	contain	has	447:449	arg1	Carrageenan					428:438	Carrageenan	428:438	Carrageenan	428:438	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	3	59	contain	has	447:449	arg2	number					459:464	a large number	451:464	a large number of hydroxyl groups	451:483	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	1	60	gly	glycopeptides	174:186	arg2	glycopeptides					174:186	glycopeptides enrichment efficiency	174:208	glycopeptides enrichment efficiency	174:208	A hydrophilic nanocomposite was synthesized by an easy route to improve glycopeptides enrichment efficiency.					
30794378	5	61	theme	serum	773:777	arg1	IgG					797:799	IgG	797:799	IgG	797:799	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	5	61	theme	serum	773:777	arg1	G					794:794	human serum immunoglobulin G	767:794	human serum immunoglobulin G (IgG) tryptic digests	767:816	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	2	62	theme	electrostatic	263:275	arg1	interaction					277:287	electrostatic interaction	263:287	electrostatic interaction	263:287	This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr).					
30794378	6	63	theme	digest	999:1004	arg1	ratio					947:951	the molar ratio	937:951	the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures	937:1013	A good selectivity was still kept even when the molar ratio of IgG and bovine serum albumin (BSA) tryptic digest mixtures reached to 1:500.					
30794378	7	64	gly	glycopeptides	1047:1059	arg2	glycopeptides					1047:1059	76 glycopeptides	1044:1059	76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites	1044:1119	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	7	64	gly	glycopeptides	1047:1059	arg2	Meanwhile					1033:1041	Meanwhile	1033:1041	Meanwhile	1033:1041	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	5	65	theme	G	794:794	arg1	digests					810:816	human serum immunoglobulin G (IgG) tryptic digests	767:816	human serum immunoglobulin G (IgG) tryptic digests	767:816	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	5	66	from	digests	810:816	arg1	glycopeptides					748:760	Thirty-four glycopeptides	736:760	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests	736:816	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	9	67	theme	glycoproteomic	1725:1738	arg1	research					1740:1747	glycoproteomic research	1725:1747	glycoproteomic research	1725:1747	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	3	68	from	phase	536:540	arg1	glycoproteomics					570:584	glycoproteomics	570:584	glycoproteomics	570:584	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	5	69	theme	Thirty-four	736:746	arg1	glycopeptides					748:760	Thirty-four glycopeptides	736:760	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests	736:816	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	2	70	from	immobilization	327:340	arg1	support					394:400	graphene oxide/poly(ethylenimine) support	360:400	graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr)	360:425	This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr).					
30794378	7	71	gly	glycoproteins	1193:1205	arg1	glycoproteins					1193:1205	129 glycoproteins	1189:1205	129 glycoproteins with 157 N-glycosylation sites	1189:1236	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	9	72	theme	enrichment	1552:1561	arg1	mg/g					1584:1587	up to ∼300 mg/g	1573:1587	up to ∼300 mg/g	1573:1587	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	9	72	theme	enrichment	1552:1561	arg1	capacity					1563:1570	a better enrichment capacity	1543:1570	a better enrichment capacity (up to ∼300 mg/g)	1543:1588	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	7	73	theme	complex	1334:1340	arg1	samples					1353:1359	complex biological samples	1334:1359	complex biological samples	1334:1359	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	7	74	attach	derived	1176:1182	arg1	glycoproteins					1193:1205	129 glycoproteins	1189:1205	129 glycoproteins with 157 N-glycosylation sites	1189:1236	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	7	74	attach	derived	1176:1182	arg2	glycopeptides					1162:1174	149 glycopeptides	1158:1174	149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites	1158:1236	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	3	75	theme	large	453:457	arg1	number					459:464	a large number	451:464	a large number of hydroxyl groups	451:483	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	3	76	theme	groups	478:483	arg1	number					459:464	a large number	451:464	a large number of hydroxyl groups	451:483	Carrageenan, which has a large number of hydroxyl groups and is fully negatively charged, is a new modified phase of hydrophilic materials in glycoproteomics.					
30794378	7	77	theme	human	1142:1146	arg1	serum					1148:1152	human serum	1142:1152	human serum	1142:1152	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	7	78	gly	N-glycosylation	1099:1113	arg2	sites					1115:1119	83 N-glycosylation sites	1096:1119	83 N-glycosylation sites	1096:1119	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	7	78	gly	N-glycosylation	1099:1113	arg2	83					1096:1097	83	1096:1097	83	1096:1097	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	8	79	theme	unique	1399:1404	arg1	repeatability					1406:1418	a unique repeatability	1397:1418	a unique repeatability	1397:1418	In addition, GO-PEI-Carr exhibited a unique repeatability and stability even after enrichment of glycopeptides for 20 times.					
30794378	5	80	theme	tryptic	802:808	arg1	digests					810:816	human serum immunoglobulin G (IgG) tryptic digests	767:816	human serum immunoglobulin G (IgG) tryptic digests	767:816	Thirty-four glycopeptides from human serum immunoglobulin G (IgG) tryptic digests were obviously observed with greatly improved signal-to-noise (S/N) ratio.					
30794378	2	81	theme	graphene	360:367	arg1	support					394:400	graphene oxide/poly(ethylenimine) support	360:400	graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr)	360:425	This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr).					
30794378	7	82	with	glycoproteins	1077:1089	arg1	sites					1115:1119	83 N-glycosylation sites	1096:1119	83 N-glycosylation sites	1096:1119	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	8	83	gly	glycopeptides	1459:1471	arg2	glycopeptides					1459:1471	glycopeptides	1459:1471	glycopeptides	1459:1471	In addition, GO-PEI-Carr exhibited a unique repeatability and stability even after enrichment of glycopeptides for 20 times.					
30794378	7	84	gly	glycopeptides	1162:1174	arg2	glycopeptides					1162:1174	149 glycopeptides	1158:1174	149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites	1158:1236	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
30794378	2	85	theme	carrageenan	345:355	arg1	immobilization					327:340	immobilization	327:340	immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr)	327:425	This new composite, prepared with a method based on electrostatic interaction, was demonstrated to be efficient for immobilization of carrageenan on graphene oxide/poly(ethylenimine) support (denoted as GO-PEI-Carr).					
30794378	9	86	theme	enrichment	1604:1613	arg1	recovery					1615:1622	an ideal enrichment recovery	1595:1622	an ideal enrichment recovery (90.8% and 109.5%)	1595:1641	It also performed a higher sensitivity (1 fmol/μL IgG), a better enrichment capacity (up to ∼300 mg/g), and an ideal enrichment recovery (90.8% and 109.5%) for glycopeptides enrichment, indicating a great potential for the application of glycoproteomic research.					
30794378	7	87	theme	mouse	1259:1263	arg1	tissues					1271:1277	mouse liver tissues	1259:1277	mouse liver tissues	1259:1277	Meanwhile, 76 glycopeptides derived from 56 glycoproteins with 83 N-glycosylation sites were identified from human serum and 149 glycopeptides derived from 129 glycoproteins with 157 N-glycosylation sites were identified from mouse liver tissues, which showed the ability to enrich glycopeptides from complex biological samples.					
31284011	1	0	theme	magnetic	167:174	arg1	@					215:215	MnFe2O4@GO@	205:215	MnFe2O4@GO@Chitosan/Cu	205:226	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	0	theme	magnetic	167:174	arg1	biocomposite					191:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	0	theme	magnetic	167:174	arg1	catalyst					281:288	an efficient heterogeneous catalyst	254:288	an efficient heterogeneous catalyst for the synthesis of triazoles	254:319	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	2	1	theme	VSM	448:450	arg1	methods					452:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	1	2	theme	chitosan-based	176:189	arg1	@					215:215	MnFe2O4@GO@	205:215	MnFe2O4@GO@Chitosan/Cu	205:226	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	2	theme	chitosan-based	176:189	arg1	biocomposite					191:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	2	theme	chitosan-based	176:189	arg1	catalyst					281:288	an efficient heterogeneous catalyst	254:288	an efficient heterogeneous catalyst for the synthesis of triazoles	254:319	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	0	3	theme	heterogeneous	107:119	arg1	nanocatalyst					121:132	a recyclable heterogeneous nanocatalyst	94:132	a recyclable heterogeneous nanocatalyst in the synthesis of triazoles	94:162	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	0	3	theme	heterogeneous	107:119	arg1	copper					84:89	copper	84:89	copper	84:89	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	1	4	theme	efficient	257:265	arg1	catalyst					281:288	an efficient heterogeneous catalyst	254:288	an efficient heterogeneous catalyst for the synthesis of triazoles	254:319	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	4	theme	efficient	257:265	arg1	biocomposite					191:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	0	5	theme	recyclable	96:105	arg1	nanocatalyst					121:132	a recyclable heterogeneous nanocatalyst	94:132	a recyclable heterogeneous nanocatalyst in the synthesis of triazoles	94:162	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	0	5	theme	recyclable	96:105	arg1	copper					84:89	copper	84:89	copper	84:89	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	2	6	theme	magnetic	359:366	arg1	properties					368:377	magnetic properties	359:377	magnetic properties	359:377	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	1	7	theme	heterogeneous	267:279	arg1	catalyst					281:288	an efficient heterogeneous catalyst	254:288	an efficient heterogeneous catalyst for the synthesis of triazoles	254:319	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	7	theme	heterogeneous	267:279	arg1	biocomposite					191:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	2	8	theme	composite	386:394	arg1	structure					326:334	structure	326:334	structure	326:334	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	2	8	theme	composite	386:394	arg1	stability					345:353	thermal stability	337:353	thermal stability	337:353	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	2	8	theme	composite	386:394	arg1	properties					368:377	magnetic properties	359:377	magnetic properties	359:377	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	2	9	theme	TEM	434:436	arg1	methods					452:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	1	10	theme	MnFe2O4	205:211	arg1	@					215:215	MnFe2O4@GO@	205:215	MnFe2O4@GO@Chitosan/Cu	205:226	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	10	theme	MnFe2O4	205:211	arg1	biocomposite					191:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	10	theme	MnFe2O4	205:211	arg1	Chitosan/Cu					216:226	Chitosan/Cu	216:226	Chitosan/Cu	216:226	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	2	11	dep	structure	326:334	arg1	The					322:324	The	322:324	The	322:324	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	1	12	theme	@	212:212	arg1	@					215:215	MnFe2O4@GO@	205:215	MnFe2O4@GO@Chitosan/Cu	205:226	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	12	theme	@	212:212	arg1	biocomposite					191:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	12	theme	@	212:212	arg1	Chitosan/Cu					216:226	Chitosan/Cu	216:226	Chitosan/Cu	216:226	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	2	13	theme	SEM	429:431	arg1	methods					452:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	3	14	theme	high	509:512	arg1	yields					514:519	high yields	509:519	high yields	509:519	The easy separation, recovery, reusability, and high yields of the products make this protocol environmentally friendly and economically attractive.					
31284011	2	15	theme	XRD	424:426	arg1	methods					452:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	3	16	theme	easy	465:468	arg1	separation					470:479	The easy separation	461:479	The easy separation	461:479	The easy separation, recovery, reusability, and high yields of the products make this protocol environmentally friendly and economically attractive.					
31284011	0	17	theme	chitosan/graphene	40:56	arg1	composite					64:72	magnetic chitosan/graphene oxide composite	31:72	magnetic chitosan/graphene oxide composite	31:72	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	0	18	theme	triazoles	154:162	arg1	synthesis					141:149	the synthesis	137:149	the synthesis of triazoles	137:162	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	2	19	theme	FT-IR	417:421	arg1	methods					452:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	0	20	theme	magnetic	31:38	arg1	composite					64:72	magnetic chitosan/graphene oxide composite	31:72	magnetic chitosan/graphene oxide composite	31:72	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	2	21	theme	TGA	439:441	arg1	methods					452:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	FT-IR, XRD, SEM, TEM, TGA, and VSM methods	417:458	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	1	22	theme	triazoles	311:319	arg1	synthesis					298:306	the synthesis	294:306	the synthesis of triazoles	294:319	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	3	23	theme	products	528:535	arg1	separation					470:479	The easy separation	461:479	The easy separation	461:479	The easy separation, recovery, reusability, and high yields of the products make this protocol environmentally friendly and economically attractive.					
31284011	3	23	theme	products	528:535	arg1	recovery					482:489	recovery	482:489	recovery	482:489	The easy separation, recovery, reusability, and high yields of the products make this protocol environmentally friendly and economically attractive.					
31284011	3	23	theme	products	528:535	arg1	reusability					492:502	reusability	492:502	reusability	492:502	The easy separation, recovery, reusability, and high yields of the products make this protocol environmentally friendly and economically attractive.					
31284011	3	23	theme	products	528:535	arg1	yields					514:519	high yields	509:519	high yields	509:519	The easy separation, recovery, reusability, and high yields of the products make this protocol environmentally friendly and economically attractive.					
31284011	0	24	theme	oxide	58:62	arg1	composite					64:72	magnetic chitosan/graphene oxide composite	31:72	magnetic chitosan/graphene oxide composite	31:72	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	1	25	used	used	246:249	arg2	biocomposite					191:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite	165:202	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	25	used	used	246:249	arg2	@					215:215	MnFe2O4@GO@	205:215	MnFe2O4@GO@Chitosan/Cu	205:226	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	1	25	used	used	246:249	arg2	catalyst					281:288	an efficient heterogeneous catalyst	254:288	an efficient heterogeneous catalyst for the synthesis of triazoles	254:319	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
31284011	2	26	theme	thermal	337:343	arg1	stability					345:353	thermal stability	337:353	thermal stability	337:353	The structure, thermal stability and magnetic properties of the composite were investigated by FT-IR, XRD, SEM, TEM, TGA, and VSM methods.					
31284011	0	27	from	nanocatalyst	121:132	arg1	synthesis					141:149	the synthesis	137:149	the synthesis of triazoles	137:162	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	0	28	theme	composite	64:72	arg1	application					16:26	application	16:26	application	16:26	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	0	28	theme	composite	64:72	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and application of magnetic chitosan/graphene oxide composite supported copper as a recyclable heterogeneous nanocatalyst in the synthesis of triazoles.					
31284011	1	29	dep	@	215:215	arg1	GO					213:214	GO	213:214	GO	213:214	A magnetic chitosan-based biocomposite, MnFe2O4@GO@Chitosan/Cu, was prepared and used as an efficient heterogeneous catalyst for the synthesis of triazoles.					
30797805	2	0	theme	electron	477:484	arg1	microscope					486:495	scanning electron microscope	468:495	scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX)	468:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	0	theme	electron	477:484	arg1	SEM					498:500	SEM	498:500	SEM	498:500	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	1	with	analysis	697:704	arg1	EDX					551:553	EDX	551:553	EDX	551:553	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	1	with	analysis	697:704	arg1	analyzer					541:548	energy dispersive X-ray analyzer	517:548	energy dispersive X-ray analyzer (EDX)	517:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	2	theme	differential	676:687	arg1	DTA					707:709	DTA	707:709	DTA	707:709	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	2	theme	differential	676:687	arg1	analysis					697:704	differential thermal analysis	676:704	differential thermal analysis (DTA)	676:710	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	3	3	theme	Freundlich	843:852	arg1	model					854:858	the Freundlich model	839:858	the Freundlich model	839:858	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	3	theme	Freundlich	843:852	arg1	best					868:871	best	868:871	best	868:871	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	1	4	theme	bio-char	245:252	arg1	Zr-CS-SAC					271:279	Zr-CS-SAC	271:279	Zr-CS-SAC	271:279	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	1	4	theme	bio-char	245:252	arg1	beads					264:268	chitosan-soya bean husk activated bio-char composite beads	211:268	chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	211:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	2	5	theme	dispersive	524:533	arg1	EDX					551:553	EDX	551:553	EDX	551:553	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	5	theme	dispersive	524:533	arg1	analyzer					541:548	energy dispersive X-ray analyzer	517:548	energy dispersive X-ray analyzer (EDX)	517:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	3	6	theme	endothermic	1041:1051	arg1	nature					1069:1074	the endothermic and spontaneous nature	1037:1074	the endothermic and spontaneous nature of the adsorption	1037:1092	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	5	7	theme	non-	1422:1425	arg1	toxicity					1427:1434	non- toxicity	1422:1434	non- toxicity	1422:1434	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	1	8	theme	composite	254:262	arg1	Zr-CS-SAC					271:279	Zr-CS-SAC	271:279	Zr-CS-SAC	271:279	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	1	8	theme	composite	254:262	arg1	beads					264:268	chitosan-soya bean husk activated bio-char composite beads	211:268	chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	211:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	1	9	theme	environmental	328:340	arg1	problems					342:349	the environmental problems	324:349	the environmental problems due to eutrophication	324:371	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	2	10	theme	diffraction	563:573	arg1	analysis					575:582	X-ray diffraction analysis	557:582	X-ray diffraction analysis (XRD)	557:588	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	10	theme	diffraction	563:573	arg1	XRD					585:587	XRD	585:587	XRD	585:587	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	3	11	theme	131.29 mg g-1	968:980	arg1	Studies					992:998	131.29 mg g-1 at 30 °C. Studies	968:998	131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters	968:1026	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	5	12	theme	composite	1261:1269	arg1	beads					1271:1275	composite beads	1261:1275	composite beads	1261:1275	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	3	13	theme	respective	933:942	arg1	capacities					944:953	respective capacities	933:953	respective capacities of 90.09	933:962	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	2	14	theme	X-ray	557:561	arg1	analysis					575:582	X-ray diffraction analysis	557:582	X-ray diffraction analysis (XRD)	557:588	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	14	theme	X-ray	557:561	arg1	XRD					585:587	XRD	585:587	XRD	585:587	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	5	15	theme	ions	1343:1346	arg1	removal					1310:1316	the removal	1306:1316	the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity	1306:1434	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	4	16	theme	kinetic	1105:1111	arg1	models					1113:1118	Different kinetic models	1095:1118	Different kinetic models	1095:1118	Different kinetic models were studied and found that pseudo-second-order kinetic data were well fitted for adsorption process.					
30797805	0	17	from	solution	138:145	arg1	recovery					86:93	the recovery	82:93	the recovery of nitrate and phosphate ions from aqueous solution	82:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	2	18	with	analysis	657:664	arg1	EDX					551:553	EDX	551:553	EDX	551:553	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	18	with	analysis	657:664	arg1	analyzer					541:548	energy dispersive X-ray analyzer	517:548	energy dispersive X-ray analyzer (EDX)	517:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	1	19	theme	due	351:353	arg1	problems					342:349	the environmental problems	324:349	the environmental problems due to eutrophication	324:371	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	2	20	theme	Brunauer-Emmett-Teller	591:612	arg1	BET					632:634	BET	632:634	BET	632:634	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	20	theme	Brunauer-Emmett-Teller	591:612	arg1	analyzer					622:629	Brunauer-Emmett-Teller surface analyzer	591:629	Brunauer-Emmett-Teller surface analyzer (BET)	591:635	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	21	theme	surface	614:620	arg1	BET					632:634	BET	632:634	BET	632:634	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	21	theme	surface	614:620	arg1	analyzer					622:629	Brunauer-Emmett-Teller surface analyzer	591:629	Brunauer-Emmett-Teller surface analyzer (BET)	591:635	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	4	22	theme	Different	1095:1103	arg1	models					1113:1118	Different kinetic models	1095:1118	Different kinetic models	1095:1118	Different kinetic models were studied and found that pseudo-second-order kinetic data were well fitted for adsorption process.					
30797805	1	23	theme	nitrate	159:165	arg1	Removal					148:154	Removal	148:154	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	148:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	0	24	theme	nitrate	98:104	arg1	recovery					86:93	the recovery	82:93	the recovery of nitrate and phosphate ions from aqueous solution	82:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	2	25	dep	Fourier	420:426	arg1	transform					428:436	transform	428:436	transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc	428:714	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	4	26	theme	pseudo-second-order	1148:1166	arg1	data					1176:1179	pseudo-second-order kinetic data	1148:1179	pseudo-second-order kinetic data	1148:1179	Different kinetic models were studied and found that pseudo-second-order kinetic data were well fitted for adsorption process.					
30797805	2	27	theme	infrared	438:445	arg1	FTIR					461:464	FTIR	461:464	FTIR	461:464	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	27	theme	infrared	438:445	arg1	spectroscopy					447:458	infrared spectroscopy	438:458	infrared spectroscopy (FTIR)	438:465	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	3	28	theme	90.09	958:962	arg1	capacities					944:953	respective capacities	933:953	respective capacities of 90.09	933:962	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	2	29	theme	scanning	468:475	arg1	microscope					486:495	scanning electron microscope	468:495	scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX)	468:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	29	theme	scanning	468:475	arg1	SEM					498:500	SEM	498:500	SEM	498:500	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	30	dep	spectroscopy	447:458	arg1	etc					712:714	etc	712:714	etc	712:714	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	31	theme	thermo-gravimetric	638:655	arg1	TGA					667:669	TGA	667:669	TGA	667:669	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	31	theme	thermo-gravimetric	638:655	arg1	analysis					657:664	thermo-gravimetric analysis	638:664	thermo-gravimetric analysis (TGA)	638:670	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	32	with	analysis	575:582	arg1	EDX					551:553	EDX	551:553	EDX	551:553	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	32	with	analysis	575:582	arg1	analyzer					541:548	energy dispersive X-ray analyzer	517:548	energy dispersive X-ray analyzer (EDX)	517:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	1	33	theme	phosphate	171:179	arg1	ions					181:184	phosphate ions	171:184	phosphate ions	171:184	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	0	34	theme	Zr4+	0:3	arg1	ions					5:8	Zr4+ ions	0:8	Zr4+ ions	0:8	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	3	35	theme	at	982:983	arg1	Studies					992:998	131.29 mg g-1 at 30 °C. Studies	968:998	131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters	968:1026	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	36	theme	spontaneous	1057:1067	arg1	nature					1069:1074	the endothermic and spontaneous nature	1037:1074	the endothermic and spontaneous nature of the adsorption	1037:1092	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	1	37	theme	ions	181:184	arg1	Removal					148:154	Removal	148:154	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	148:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	0	38	theme	chitosan-soya	19:31	arg1	beads					72:76	chitosan-soya bean husk activated bio-char composite beads	19:76	chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution	19:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	4	39	theme	adsorption	1202:1211	arg1	process					1213:1219	adsorption process	1202:1219	adsorption process	1202:1219	Different kinetic models were studied and found that pseudo-second-order kinetic data were well fitted for adsorption process.					
30797805	0	40	theme	ions	120:123	arg1	recovery					86:93	the recovery	82:93	the recovery of nitrate and phosphate ions from aqueous solution	82:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	2	41	with	analysis	503:510	arg1	EDX					551:553	EDX	551:553	EDX	551:553	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	41	with	analysis	503:510	arg1	analyzer					541:548	energy dispersive X-ray analyzer	517:548	energy dispersive X-ray analyzer (EDX)	517:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	3	42	theme	D-R	779:781	arg1	isotherms					783:791	Langmuir, Freundlich and D-R isotherms	754:791	Langmuir, Freundlich and D-R isotherms	754:791	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	43	theme	adsorbates	886:895	arg1	ions					923:926	both the adsorbates of nitrate and phosphates ions	877:926	both the adsorbates of nitrate and phosphates ions	877:926	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	5	44	theme	good	1364:1367	arg1	efficiency					1377:1386	good removal efficiency	1364:1386	good removal efficiency	1364:1386	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	0	45	theme	phosphate	110:118	arg1	ions					120:123	phosphate ions	110:123	phosphate ions	110:123	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	5	46	from	water	1353:1357	arg1	removal					1310:1316	the removal	1306:1316	the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity	1306:1434	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	1	47	theme	Zr4+	192:195	arg1	ions					197:200	Zr4+ ions	192:200	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	192:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	0	48	theme	husk	38:41	arg1	beads					72:76	chitosan-soya bean husk activated bio-char composite beads	19:76	chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution	19:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	5	49	theme	nitrate	1321:1327	arg1	removal					1310:1316	the removal	1306:1316	the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity	1306:1434	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	0	50	theme	aqueous	130:136	arg1	solution					138:145	aqueous solution	130:145	aqueous solution	130:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	5	51	theme	removal	1369:1375	arg1	efficiency					1377:1386	good removal efficiency	1364:1386	good removal efficiency	1364:1386	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	0	52	theme	bean	33:36	arg1	beads					72:76	chitosan-soya bean husk activated bio-char composite beads	19:76	chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution	19:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	3	53	theme	equilibrium	732:742	arg1	models					744:749	The adsorption equilibrium models	717:749	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms	717:791	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	2	54	theme	energy	517:522	arg1	EDX					551:553	EDX	551:553	EDX	551:553	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	54	theme	energy	517:522	arg1	analyzer					541:548	energy dispersive X-ray analyzer	517:548	energy dispersive X-ray analyzer (EDX)	517:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	4	55	theme	kinetic	1168:1174	arg1	data					1176:1179	pseudo-second-order kinetic data	1148:1179	pseudo-second-order kinetic data	1148:1179	Different kinetic models were studied and found that pseudo-second-order kinetic data were well fitted for adsorption process.					
30797805	1	56	theme	embedded	202:209	arg1	ions					197:200	Zr4+ ions	192:200	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	192:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	0	57	theme	bio-char	53:60	arg1	beads					72:76	chitosan-soya bean husk activated bio-char composite beads	19:76	chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution	19:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	2	58	theme	microscope	486:495	arg1	analysis					503:510	scanning electron microscope (SEM) analysis	468:510	scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX)	468:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	3	59	theme	phosphates	912:921	arg1	ions					923:926	both the adsorbates of nitrate and phosphates ions	877:926	both the adsorbates of nitrate and phosphates ions	877:926	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	0	60	theme	activated	43:51	arg1	beads					72:76	chitosan-soya bean husk activated bio-char composite beads	19:76	chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution	19:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	1	61	theme	batch	301:305	arg1	mode					307:310	batch mode	301:310	batch mode	301:310	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	3	62	theme	adsorption	1083:1092	arg1	nature					1069:1074	the endothermic and spontaneous nature	1037:1074	the endothermic and spontaneous nature of the adsorption	1037:1092	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	2	63	with	spectroscopy	447:458	arg1	EDX					551:553	EDX	551:553	EDX	551:553	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	63	with	spectroscopy	447:458	arg1	analyzer					541:548	energy dispersive X-ray analyzer	517:548	energy dispersive X-ray analyzer (EDX)	517:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	3	64	theme	thermodynamic	1003:1015	arg1	parameters					1017:1026	thermodynamic parameters	1003:1026	thermodynamic parameters	1003:1026	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	65	theme	30 °C.	985:990	arg1	Studies					992:998	131.29 mg g-1 at 30 °C. Studies	968:998	131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters	968:1026	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	66	theme	Freundlich	764:773	arg1	isotherms					783:791	Langmuir, Freundlich and D-R isotherms	754:791	Langmuir, Freundlich and D-R isotherms	754:791	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	67	theme	nitrate	900:906	arg1	phosphates					912:921	phosphates	912:921	phosphates	912:921	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	67	theme	nitrate	900:906	arg1	adsorbates					886:895	the adsorbates	882:895	the adsorbates of nitrate	882:906	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	68	from	Studies	992:998	arg1	30 °C.					985:990	131.29 mg g-1 at 30 °C. Studies	968:998	131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters	968:1026	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	68	from	Studies	992:998	arg1	131.29 mg g-1					968:980	131.29 mg g-1 at 30 °C. Studies	968:998	131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters	968:1026	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	68	from	Studies	992:998	arg1	parameters					1017:1026	thermodynamic parameters	1003:1026	thermodynamic parameters	1003:1026	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	3	68	from	Studies	992:998	arg1	at					982:983	131.29 mg g-1 at 30 °C. Studies	968:998	131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters	968:1026	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	1	69	theme	chitosan-soya	211:223	arg1	Zr-CS-SAC					271:279	Zr-CS-SAC	271:279	Zr-CS-SAC	271:279	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	1	69	theme	chitosan-soya	211:223	arg1	beads					264:268	chitosan-soya bean husk activated bio-char composite beads	211:268	chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	211:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	3	70	theme	adsorption	721:730	arg1	models					744:749	The adsorption equilibrium models	717:749	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms	717:791	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	1	71	dep	embedded	202:209	arg1	Zr-CS-SAC					271:279	Zr-CS-SAC	271:279	Zr-CS-SAC	271:279	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	1	71	dep	embedded	202:209	arg1	beads					264:268	chitosan-soya bean husk activated bio-char composite beads	211:268	chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	211:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	3	72	theme	isotherms	783:791	arg1	models					744:749	The adsorption equilibrium models	717:749	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms	717:791	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	5	73	theme	phosphate	1333:1341	arg1	ions					1343:1346	phosphate ions	1333:1346	phosphate ions	1333:1346	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	1	74	theme	bean	225:228	arg1	Zr-CS-SAC					271:279	Zr-CS-SAC	271:279	Zr-CS-SAC	271:279	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	1	74	theme	bean	225:228	arg1	beads					264:268	chitosan-soya bean husk activated bio-char composite beads	211:268	chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	211:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	2	75	with	analyzer	622:629	arg1	EDX					551:553	EDX	551:553	EDX	551:553	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	75	with	analyzer	622:629	arg1	analyzer					541:548	energy dispersive X-ray analyzer	517:548	energy dispersive X-ray analyzer (EDX)	517:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	76	theme	X-ray	535:539	arg1	EDX					551:553	EDX	551:553	EDX	551:553	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	76	theme	X-ray	535:539	arg1	analyzer					541:548	energy dispersive X-ray analyzer	517:548	energy dispersive X-ray analyzer (EDX)	517:554	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	1	77	theme	husk	230:233	arg1	Zr-CS-SAC					271:279	Zr-CS-SAC	271:279	Zr-CS-SAC	271:279	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	1	77	theme	husk	230:233	arg1	beads					264:268	chitosan-soya bean husk activated bio-char composite beads	211:268	chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	211:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	0	78	theme	composite	62:70	arg1	beads					72:76	chitosan-soya bean husk activated bio-char composite beads	19:76	chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution	19:145	Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads for the recovery of nitrate and phosphate ions from aqueous solution.					
30797805	3	79	theme	Langmuir	754:761	arg1	isotherms					783:791	Langmuir, Freundlich and D-R isotherms	754:791	Langmuir, Freundlich and D-R isotherms	754:791	The adsorption equilibrium models of Langmuir, Freundlich and D-R isotherms were evaluated and the results described that the Freundlich model was the best for both the adsorbates of nitrate and phosphates ions with respective capacities of 90.09 and 131.29 mg g-1 at 30 °C. Studies on thermodynamic parameters revealed the endothermic and spontaneous nature of the adsorption.					
30797805	2	80	theme	thermal	689:695	arg1	DTA					707:709	DTA	707:709	DTA	707:709	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	2	80	theme	thermal	689:695	arg1	analysis					697:704	differential thermal analysis	676:704	differential thermal analysis (DTA)	676:710	The adsorbent was well characterized by using Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) analysis with energy dispersive X-ray analyzer (EDX), X-ray diffraction analysis (XRD), Brunauer-Emmett-Teller surface analyzer (BET), thermo-gravimetric analysis (TGA) and differential thermal analysis (DTA) etc.					
30797805	5	81	with	water	1353:1357	arg1	recyclability					1404:1416	recyclability	1404:1416	recyclability	1404:1416	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	5	81	with	water	1353:1357	arg1	toxicity					1427:1434	non- toxicity	1422:1434	non- toxicity	1422:1434	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	5	81	with	water	1353:1357	arg1	adsorbability					1389:1401	adsorbability	1389:1401	adsorbability	1389:1401	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	5	81	with	water	1353:1357	arg1	efficiency					1377:1386	good removal efficiency	1364:1386	good removal efficiency	1364:1386	These results suggested that Zr-CS-SAC composite beads as a promising adsorbent for the removal of nitrate and phosphate ions from water with good removal efficiency, adsorbability, recyclability and non- toxicity.					
30797805	1	82	theme	activated	235:243	arg1	Zr-CS-SAC					271:279	Zr-CS-SAC	271:279	Zr-CS-SAC	271:279	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
30797805	1	82	theme	activated	235:243	arg1	beads					264:268	chitosan-soya bean husk activated bio-char composite beads	211:268	chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC)	211:280	Removal of nitrate and phosphate ions using Zr4+ ions embedded chitosan-soya bean husk activated bio-char composite beads (Zr-CS-SAC) was carried out by batch mode to overcome the environmental problems due to eutrophication.					
31125651	2	0	theme	total	500:504	arg1	acidity					506:512	total acidity	500:512	total acidity from 27 to 7 days	500:530	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	4	1	theme	oil	700:702	arg1	solubility					704:713	oil solubility	700:713	oil solubility	700:713	The kind of fermentation did not change the gelatinization parameters, viscosity breakdown and oil solubility.					
31125651	4	1	theme	oil	700:702	arg1	parameters					664:673	the gelatinization parameters	645:673	the gelatinization parameters	645:673	The kind of fermentation did not change the gelatinization parameters, viscosity breakdown and oil solubility.					
31125651	1	2	from	fermentation	184:195	arg1	properties					224:233	waxy maize starch (WMS) properties	200:233	waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics	200:374	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	1	3	theme	spontaneous	155:165	arg1	fermentation					184:195	spontaneous or back-slopping fermentation	155:195	spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics	155:374	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	6	4	theme	Back-slopping	904:916	arg1	fermentation					918:929	Back-slopping fermentation	904:929	Back-slopping fermentation	904:929	Back-slopping fermentation can speed up the process of the modification of waxy maize starch, and change its functional properties, which can extend the WMS industrial applications.					
31125651	5	5	theme	time	803:806	arg1	values					830:835	increasing peak time and paste temperature values	787:835	increasing peak time and paste temperature values	787:835	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	1	6	theme	pasting	250:256	arg1	properties					258:267	thermal and pasting properties	238:267	thermal and pasting properties	238:267	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	6	7	theme	maize	984:988	arg1	starch					990:995	waxy maize starch	979:995	waxy maize starch	979:995	Back-slopping fermentation can speed up the process of the modification of waxy maize starch, and change its functional properties, which can extend the WMS industrial applications.					
31125651	5	8	theme	final	745:749	arg1	viscosity					751:759	final viscosity	745:759	final viscosity	745:759	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	1	9	theme	morphological	346:358	arg1	characteristics					360:374	morphological characteristics	346:374	morphological characteristics	346:374	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	6	10	theme	starch	990:995	arg1	modification					963:974	the modification	959:974	the modification of waxy maize starch	959:995	Back-slopping fermentation can speed up the process of the modification of waxy maize starch, and change its functional properties, which can extend the WMS industrial applications.					
31125651	5	11	theme	more	845:848	arg1	differences					862:872	more statistical differences	845:872	more statistical differences	845:872	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	1	12	theme	back-slopping	170:182	arg1	fermentation					184:195	spontaneous or back-slopping fermentation	155:195	spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics	155:374	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	2	13	from	%	495:495	arg1	7 days					525:530	7 days	525:530	7 days	525:530	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	5	14	theme	temperature	818:828	arg1	values					830:835	increasing peak time and paste temperature values	787:835	increasing peak time and paste temperature values	787:835	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	0	15	theme	maize	5:9	arg1	starch					11:16	Waxy maize starch	0:16	Waxy maize starch	0:16	Waxy maize starch modified by sun-drying after spontaneous or backslopping fermentation.					
31125651	6	16	theme	modification	963:974	arg1	process					948:954	the process	944:954	the process of the modification of waxy maize starch	944:995	Back-slopping fermentation can speed up the process of the modification of waxy maize starch, and change its functional properties, which can extend the WMS industrial applications.					
31125651	5	17	theme	setback	765:771	arg1	values					773:778	setback values	765:778	setback values	765:778	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	6	18	theme	industrial	1061:1070	arg1	applications					1072:1083	the WMS industrial applications	1053:1083	the WMS industrial applications	1053:1083	Back-slopping fermentation can speed up the process of the modification of waxy maize starch, and change its functional properties, which can extend the WMS industrial applications.					
31125651	0	19	theme	Waxy	0:3	arg1	starch					11:16	Waxy maize starch	0:16	Waxy maize starch	0:16	Waxy maize starch modified by sun-drying after spontaneous or backslopping fermentation.					
31125651	1	20	theme	water	270:274	arg1	activity					276:283	water activity	270:283	water activity	270:283	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	1	21	theme	waxy	200:203	arg1	properties					224:233	waxy maize starch (WMS) properties	200:233	waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics	200:374	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	6	22	theme	waxy	979:982	arg1	starch					990:995	waxy maize starch	979:995	waxy maize starch	979:995	Back-slopping fermentation can speed up the process of the modification of waxy maize starch, and change its functional properties, which can extend the WMS industrial applications.					
31125651	3	23	theme	surface	556:562	arg1	degradation					564:574	surface degradation	556:574	surface degradation by the fermentation process	556:602	WMS was susceptible to surface degradation by the fermentation process.					
31125651	2	24	theme	back-slopping	436:448	arg1	fermentation					450:461	back-slopping fermentation	436:461	back-slopping fermentation	436:461	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	1	25	theme	starch	211:216	arg1	properties					224:233	waxy maize starch (WMS) properties	200:233	waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics	200:374	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	5	26	theme	increasing	787:796	arg1	values					830:835	increasing peak time and paste temperature values	787:835	increasing peak time and paste temperature values	787:835	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	1	27	theme	chemical	289:296	arg1	composition					298:308	chemical composition	289:308	chemical composition	289:308	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	5	28	theme	peak	729:732	arg1	viscosity					734:742	peak viscosity	729:742	peak viscosity	729:742	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	5	29	theme	statistical	850:860	arg1	differences					862:872	more statistical differences	845:872	more statistical differences	845:872	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	2	30	theme	microbial	381:389	arg1	metabolism					391:400	The microbial metabolism	377:400	The microbial metabolism	377:400	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	4	31	theme	gelatinization	649:662	arg1	solubility					704:713	oil solubility	700:713	oil solubility	700:713	The kind of fermentation did not change the gelatinization parameters, viscosity breakdown and oil solubility.					
31125651	4	31	theme	gelatinization	649:662	arg1	parameters					664:673	the gelatinization parameters	645:673	the gelatinization parameters	645:673	The kind of fermentation did not change the gelatinization parameters, viscosity breakdown and oil solubility.					
31125651	4	31	theme	gelatinization	649:662	arg1	breakdown					686:694	viscosity breakdown	676:694	viscosity breakdown	676:694	The kind of fermentation did not change the gelatinization parameters, viscosity breakdown and oil solubility.					
31125651	2	32	theme	chemical	411:418	arg1	composition					420:430	chemical composition	411:430	chemical composition	411:430	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	2	33	dep	7 days	525:530	arg1	to					522:523	to	522:523	to	522:523	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	1	34	theme	WMS	219:221	arg1	properties					224:233	waxy maize starch (WMS) properties	200:233	waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics	200:374	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	0	35	theme	spontaneous	47:57	arg1	fermentation					75:86	spontaneous or backslopping fermentation	47:86	spontaneous or backslopping fermentation	47:86	Waxy maize starch modified by sun-drying after spontaneous or backslopping fermentation.					
31125651	3	36	theme	fermentation	583:594	arg1	process					596:602	the fermentation process	579:602	the fermentation process	579:602	WMS was susceptible to surface degradation by the fermentation process.					
31125651	1	37	theme	swelling	311:318	arg1	power					320:324	swelling power	311:324	swelling power	311:324	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	4	38	theme	viscosity	676:684	arg1	parameters					664:673	the gelatinization parameters	645:673	the gelatinization parameters	645:673	The kind of fermentation did not change the gelatinization parameters, viscosity breakdown and oil solubility.					
31125651	4	38	theme	viscosity	676:684	arg1	breakdown					686:694	viscosity breakdown	676:694	viscosity breakdown	676:694	The kind of fermentation did not change the gelatinization parameters, viscosity breakdown and oil solubility.					
31125651	5	39	theme	peak	798:801	arg1	values					830:835	increasing peak time and paste temperature values	787:835	increasing peak time and paste temperature values	787:835	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	6	40	theme	WMS	1057:1059	arg1	applications					1072:1083	the WMS industrial applications	1053:1083	the WMS industrial applications	1053:1083	Back-slopping fermentation can speed up the process of the modification of waxy maize starch, and change its functional properties, which can extend the WMS industrial applications.					
31125651	0	41	theme	backslopping	62:73	arg1	fermentation					75:86	spontaneous or backslopping fermentation	47:86	spontaneous or backslopping fermentation	47:86	Waxy maize starch modified by sun-drying after spontaneous or backslopping fermentation.					
31125651	2	42	from	7 days	525:530	arg1	acidity					506:512	total acidity	500:512	total acidity from 27 to 7 days	500:530	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	2	42	from	7 days	525:530	arg1	%					495:495	4%	494:495	4% of total acidity from 27 to 7 days	494:530	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	1	43	theme	maize	205:209	arg1	properties					224:233	waxy maize starch (WMS) properties	200:233	waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics	200:374	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	1	44	theme	sun-drying	138:147	arg1	influence					125:133	the influence	121:133	the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics	121:374	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
31125651	6	45	theme	functional	1013:1022	arg1	properties					1024:1033	its functional properties	1009:1033	its functional properties	1009:1033	Back-slopping fermentation can speed up the process of the modification of waxy maize starch, and change its functional properties, which can extend the WMS industrial applications.					
31125651	2	46	theme	acidity	506:512	arg1	acidity					506:512	total acidity	500:512	total acidity from 27 to 7 days	500:530	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	2	46	theme	acidity	506:512	arg1	%					495:495	4%	494:495	4% of total acidity from 27 to 7 days	494:530	The microbial metabolism affected chemical composition, as back-slopping fermentation reduced the time for achieving 4% of total acidity from 27 to 7 days.					
31125651	4	47	theme	fermentation	617:628	arg1	kind					609:612	The kind	605:612	The kind of fermentation	605:628	The kind of fermentation did not change the gelatinization parameters, viscosity breakdown and oil solubility.					
31125651	5	48	theme	paste	812:816	arg1	temperature					818:828	paste temperature	812:828	paste temperature	812:828	FS decreased peak viscosity, final viscosity and setback values, while increasing peak time and paste temperature values, showed more statistical differences with respect to WMS than BSF.					
31125651	1	49	theme	thermal	238:244	arg1	properties					258:267	thermal and pasting properties	238:267	thermal and pasting properties	238:267	This study aimed to investigate the influence of sun-drying after spontaneous or back-slopping fermentation on waxy maize starch (WMS) properties by thermal and pasting properties, water activity and chemical composition, swelling power and solubility, and morphological characteristics.					
32066258	8	0	theme	faecal	1142:1147	arg1	samples					1149:1155	faecal samples	1142:1155	faecal samples	1142:1155	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	11	1	theme	relative	1535:1542	arg1	abundance					1544:1552	the relative abundance	1531:1552	the relative abundance of genus Allobaculum (Firmicutes), putative butyrate-producing bacteria	1531:1624	FOS supplementation increased the relative abundance of genus Allobaculum (Firmicutes), putative butyrate-producing bacteria.					
32066258	8	2	theme	sensitised	1169:1178	arg1	mice					1180:1183	sensitised mice	1169:1183	sensitised mice fed the control diet (P=0.03)	1169:1213	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	12	3	theme	sensitised	1809:1818	arg1	controls					1820:1827	sensitised controls	1809:1827	sensitised controls	1809:1827	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	3	4	theme	allergy	680:686	arg1	treatment					662:670	the treatment	658:670	the treatment of food allergy	658:686	It is thus far unknown which microbial alterations are involved in successful outcomes of OIT with prebiotic supplementation for the treatment of food allergy.					
32066258	10	5	theme	relative	1402:1409	arg1	abundance					1411:1419	an increased relative abundance	1389:1419	an increased relative abundance of the dysbiosis-associated phylum Proteobacteria	1389:1469	OIT mice showed an increased relative abundance of the dysbiosis-associated phylum Proteobacteria compared to the OIT+FOS mice.					
32066258	11	6	theme	Allobaculum	1563:1573	arg1	abundance					1544:1552	the relative abundance	1531:1552	the relative abundance of genus Allobaculum (Firmicutes), putative butyrate-producing bacteria	1531:1624	FOS supplementation increased the relative abundance of genus Allobaculum (Firmicutes), putative butyrate-producing bacteria.					
32066258	1	7	theme	immunotherapy	190:202	arg1	efficacy					173:180	enhanced efficacy	164:180	enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation	164:376	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	8	8	theme	control	1193:1199	arg1	P=0.03					1207:1212	P=0.03	1207:1212	P=0.03	1207:1212	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	8	8	theme	control	1193:1199	arg1	diet					1201:1204	the control diet	1189:1204	the control diet (P=0.03)	1189:1213	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	1	9	theme	mast	354:357	arg1	degranulation					364:376	mast cell degranulation	354:376	mast cell degranulation	354:376	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	15	10	theme	microbial	2224:2232	arg1	modulation					2234:2243	microbial modulation	2224:2243	microbial modulation	2224:2243	Future research should confirm whether there is a causal relationship between microbial modulation and the reduction in acute allergic symptoms induced by OIT+FOS.					
32066258	14	11	theme	propionic	2130:2138	arg1	acid					2140:2143	propionic acid	2130:2143	propionic acid	2130:2143	OIT+FOS induced a microbial profile closely linked to non-allergic mice and increased concentrations of butyric acid and propionic acid.					
32066258	9	12	located	observed	1294:1301	arg1	contrast					1219:1226	contrast	1219:1226	contrast	1219:1226	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	9	12	located	observed	1294:1301	arg2	level					1236:1240	a high level	1229:1240	a high level of resemblance in bacterial community structure	1229:1288	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	3	13	theme	microbial	558:566	arg1	alterations					568:578	microbial alterations	558:578	microbial alterations	558:578	It is thus far unknown which microbial alterations are involved in successful outcomes of OIT with prebiotic supplementation for the treatment of food allergy.					
32066258	15	14	from	relationship	2203:2214	arg1	symptoms					2281:2288	acute allergic symptoms	2266:2288	acute allergic symptoms induced by OIT+FOS	2266:2307	Future research should confirm whether there is a causal relationship between microbial modulation and the reduction in acute allergic symptoms induced by OIT+FOS.					
32066258	13	15	theme	butyric	1961:1967	arg1	acid					1969:1972	butyric acid	1961:1972	butyric acid	1961:1972	OIT+FOS mice had increased butyric acid and propionic acid concentrations.					
32066258	12	16	theme	unclassified	1757:1768	arg1	Firmicutes					1785:1794	Firmicutes	1785:1794	Firmicutes	1785:1794	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	12	16	theme	unclassified	1757:1768	arg1	Clostridiales					1770:1782	unclassified Clostridiales	1757:1782	unclassified Clostridiales (Firmicutes)	1757:1795	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	9	17	theme	resemblance	1245:1255	arg1	level					1236:1240	a high level	1229:1240	a high level of resemblance in bacterial community structure	1229:1288	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	8	18	from	supplementation	1054:1068	arg1	mice					1084:1087	sensitised mice	1073:1087	sensitised mice	1073:1087	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	10	19	theme	dysbiosis-associated	1428:1447	arg1	Proteobacteria					1456:1469	the dysbiosis-associated phylum Proteobacteria	1424:1469	the dysbiosis-associated phylum Proteobacteria	1424:1469	OIT mice showed an increased relative abundance of the dysbiosis-associated phylum Proteobacteria compared to the OIT+FOS mice.					
32066258	15	20	from	reduction	2253:2261	arg1	symptoms					2281:2288	acute allergic symptoms	2266:2288	acute allergic symptoms induced by OIT+FOS	2266:2307	Future research should confirm whether there is a causal relationship between microbial modulation and the reduction in acute allergic symptoms induced by OIT+FOS.					
32066258	13	21	theme	OIT+FOS	1934:1940	arg1	mice					1942:1945	OIT+FOS mice	1934:1945	OIT+FOS mice	1934:1945	OIT+FOS mice had increased butyric acid and propionic acid concentrations.					
32066258	0	22	theme	allergic	67:74	arg1	mice					76:79	cow's milk allergic mice	56:79	cow's milk allergic mice	56:79	Altered microbial community structure and metabolism in cow's milk allergic mice treated with oral immunotherapy and fructo-oligosaccharides.					
32066258	9	23	theme	bacterial	1260:1268	arg1	structure					1280:1288	bacterial community structure	1260:1288	bacterial community structure	1260:1288	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	1	24	theme	enhanced	164:171	arg1	efficacy					173:180	enhanced efficacy	164:180	enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation	164:376	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	12	25	theme	pro-inflammatory	1726:1741	arg1	Rikenellaceae					1687:1699	the genera's unclassified Rikenellaceae	1661:1699	the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria)	1661:1751	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	12	25	theme	pro-inflammatory	1726:1741	arg1	bacteria					1743:1750	putative pro-inflammatory bacteria	1717:1750	putative pro-inflammatory bacteria	1717:1750	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	12	26	theme	unclassified	1674:1685	arg1	Rikenellaceae					1687:1699	the genera's unclassified Rikenellaceae	1661:1699	the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria)	1661:1751	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	12	26	theme	unclassified	1674:1685	arg1	bacteria					1743:1750	putative pro-inflammatory bacteria	1717:1750	putative pro-inflammatory bacteria	1717:1750	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	6	27	theme	microbiota	946:955	arg1	composition					957:967	gut microbiota composition	942:967	gut microbiota composition	942:967	After three weeks, faecal samples were collected to analyse gut microbiota composition using 16S rRNA sequencing.					
32066258	2	28	theme	short-chain	496:506	arg1	SCFA					521:524	SCFA	521:524	SCFA	521:524	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	2	28	theme	short-chain	496:506	arg1	acids					514:518	short-chain fatty acids	496:518	short-chain fatty acids (SCFA)	496:525	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	0	29	theme	oral	94:97	arg1	immunotherapy					99:111	oral immunotherapy	94:111	oral immunotherapy	94:111	Altered microbial community structure and metabolism in cow's milk allergic mice treated with oral immunotherapy and fructo-oligosaccharides.					
32066258	14	30	theme	non-allergic	2063:2074	arg1	mice					2076:2079	non-allergic mice	2063:2079	non-allergic mice	2063:2079	OIT+FOS induced a microbial profile closely linked to non-allergic mice and increased concentrations of butyric acid and propionic acid.					
32066258	10	31	theme	Proteobacteria	1456:1469	arg1	abundance					1411:1419	an increased relative abundance	1389:1419	an increased relative abundance of the dysbiosis-associated phylum Proteobacteria	1389:1469	OIT mice showed an increased relative abundance of the dysbiosis-associated phylum Proteobacteria compared to the OIT+FOS mice.					
32066258	0	32	theme	Altered	0:6	arg1	structure					28:36	Altered microbial community structure	0:36	Altered microbial community structure	0:36	Altered microbial community structure and metabolism in cow's milk allergic mice treated with oral immunotherapy and fructo-oligosaccharides.					
32066258	4	33	from	changes	710:716	arg1	composition					736:746	microbiota composition	725:746	microbiota composition	725:746	To explore potential changes in the microbiota composition and availability of SCFA induced by OIT+FOS.					
32066258	4	33	from	changes	710:716	arg1	availability					752:763	availability	752:763	availability	752:763	To explore potential changes in the microbiota composition and availability of SCFA induced by OIT+FOS.					
32066258	0	34	theme	community	18:26	arg1	structure					28:36	Altered microbial community structure	0:36	Altered microbial community structure	0:36	Altered microbial community structure and metabolism in cow's milk allergic mice treated with oral immunotherapy and fructo-oligosaccharides.					
32066258	9	35	theme	control	1330:1336	arg1	mice					1338:1341	the non-sensitised control mice	1311:1341	the non-sensitised control mice	1311:1341	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	10	36	theme	OIT+FOS	1487:1493	arg1	mice					1495:1498	the OIT+FOS mice	1483:1498	the OIT+FOS mice	1483:1498	OIT mice showed an increased relative abundance of the dysbiosis-associated phylum Proteobacteria compared to the OIT+FOS mice.					
32066258	3	37	theme	OIT	619:621	arg1	outcomes					607:614	successful outcomes	596:614	successful outcomes of OIT with prebiotic supplementation for the treatment of food allergy	596:686	It is thus far unknown which microbial alterations are involved in successful outcomes of OIT with prebiotic supplementation for the treatment of food allergy.					
32066258	1	38	from	reduction	319:327	arg1	degranulation					364:376	mast cell degranulation	354:376	mast cell degranulation	354:376	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	1	38	from	reduction	319:327	arg1	symptoms					341:348	clinical symptoms	332:348	clinical symptoms	332:348	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	8	39	theme	sensitised	1073:1082	arg1	mice					1084:1087	sensitised mice	1073:1087	sensitised mice	1073:1087	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	8	40	from	structure	1129:1137	arg1	samples					1149:1155	faecal samples	1142:1155	faecal samples	1142:1155	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	12	41	theme	Lactobacillus	1860:1872	arg1	abundance					1847:1855	the abundance	1843:1855	the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria)	1843:1915	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	2	42	theme	bacterial	436:444	arg1	composition					446:456	bacterial composition	436:456	bacterial composition	436:456	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	14	43	theme	acid	2121:2124	arg1	concentrations					2095:2108	concentrations	2095:2108	concentrations of butyric acid and propionic acid	2095:2143	OIT+FOS induced a microbial profile closely linked to non-allergic mice and increased concentrations of butyric acid and propionic acid.					
32066258	9	44	theme	OIT+FOS	1351:1357	arg1	mice					1367:1370	the OIT+FOS treated mice	1347:1370	the OIT+FOS treated mice	1347:1370	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	14	45	attach	linked	2053:2058	arg2	profile					2037:2043	a microbial profile	2025:2043	a microbial profile closely linked to non-allergic mice	2025:2079	OIT+FOS induced a microbial profile closely linked to non-allergic mice and increased concentrations of butyric acid and propionic acid.					
32066258	14	45	attach	linked	2053:2058	arg1	mice					2076:2079	non-allergic mice	2063:2079	non-allergic mice	2063:2079	OIT+FOS induced a microbial profile closely linked to non-allergic mice and increased concentrations of butyric acid and propionic acid.					
32066258	13	46	theme	propionic	1978:1986	arg1	acid					1988:1991	propionic acid	1978:1991	propionic acid	1978:1991	OIT+FOS mice had increased butyric acid and propionic acid concentrations.					
32066258	4	47	theme	SCFA	768:771	arg1	composition					736:746	microbiota composition	725:746	microbiota composition	725:746	To explore potential changes in the microbiota composition and availability of SCFA induced by OIT+FOS.					
32066258	4	47	theme	SCFA	768:771	arg1	availability					752:763	availability	752:763	availability	752:763	To explore potential changes in the microbiota composition and availability of SCFA induced by OIT+FOS.					
32066258	11	48	theme	putative	1589:1596	arg1	bacteria					1617:1624	putative butyrate-producing bacteria	1589:1624	putative butyrate-producing bacteria	1589:1624	FOS supplementation increased the relative abundance of genus Allobaculum (Firmicutes), putative butyrate-producing bacteria.					
32066258	11	48	theme	putative	1589:1596	arg1	Allobaculum					1563:1573	genus Allobaculum	1557:1573	genus Allobaculum (Firmicutes)	1557:1586	FOS supplementation increased the relative abundance of genus Allobaculum (Firmicutes), putative butyrate-producing bacteria.					
32066258	10	49	dep	showed	1382:1387	arg1	compared					1471:1478	compared	1471:1478	showed an increased relative abundance of the dysbiosis-associated phylum Proteobacteria compared to the OIT+FOS mice	1382:1498	OIT mice showed an increased relative abundance of the dysbiosis-associated phylum Proteobacteria compared to the OIT+FOS mice.					
32066258	8	50	theme	overall	1101:1107	arg1	structure					1129:1137	the overall microbial community structure	1097:1137	the overall microbial community structure in faecal samples	1097:1155	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	3	51	theme	prebiotic	628:636	arg1	supplementation					638:652	prebiotic supplementation	628:652	prebiotic supplementation for the treatment of food allergy	628:686	It is thus far unknown which microbial alterations are involved in successful outcomes of OIT with prebiotic supplementation for the treatment of food allergy.					
32066258	8	52	theme	community	1119:1127	arg1	structure					1129:1137	the overall microbial community structure	1097:1137	the overall microbial community structure in faecal samples	1097:1155	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	6	53	theme	rRNA	979:982	arg1	sequencing					984:993	16S rRNA sequencing	975:993	16S rRNA sequencing	975:993	After three weeks, faecal samples were collected to analyse gut microbiota composition using 16S rRNA sequencing.					
32066258	3	54	theme	food	675:678	arg1	allergy					680:686	food allergy	675:686	food allergy	675:686	It is thus far unknown which microbial alterations are involved in successful outcomes of OIT with prebiotic supplementation for the treatment of food allergy.					
32066258	13	55	theme	acid	1988:1991	arg1	concentrations					1993:2006	increased butyric acid and propionic acid concentrations	1951:2006	increased butyric acid and propionic acid concentrations	1951:2006	OIT+FOS mice had increased butyric acid and propionic acid concentrations.					
32066258	10	56	theme	OIT	1373:1375	arg1	mice					1377:1380	OIT mice	1373:1380	OIT mice	1373:1380	OIT mice showed an increased relative abundance of the dysbiosis-associated phylum Proteobacteria compared to the OIT+FOS mice.					
32066258	1	57	theme	allergic	290:297	arg1	mice					299:302	cow's milk allergic mice	279:302	cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation	279:376	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	10	58	theme	increased	1392:1400	arg1	abundance					1411:1419	an increased relative abundance	1389:1419	an increased relative abundance of the dysbiosis-associated phylum Proteobacteria	1389:1469	OIT mice showed an increased relative abundance of the dysbiosis-associated phylum Proteobacteria compared to the OIT+FOS mice.					
32066258	1	59	theme	oral	185:188	arg1	OIT					205:207	OIT	205:207	OIT	205:207	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	1	59	theme	oral	185:188	arg1	immunotherapy					190:202	oral immunotherapy	185:202	oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation	185:376	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	6	60	theme	gut	942:944	arg1	composition					957:967	gut microbiota composition	942:967	gut microbiota composition	942:967	After three weeks, faecal samples were collected to analyse gut microbiota composition using 16S rRNA sequencing.					
32066258	12	61	dep	bacteria	1743:1750	arg1	Bacteroidetes					1702:1714	Bacteroidetes	1702:1714	Bacteroidetes	1702:1714	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	3	62	with	outcomes	607:614	arg1	supplementation					638:652	prebiotic supplementation	628:652	prebiotic supplementation for the treatment of food allergy	628:686	It is thus far unknown which microbial alterations are involved in successful outcomes of OIT with prebiotic supplementation for the treatment of food allergy.					
32066258	15	63	theme	acute	2266:2270	arg1	symptoms					2281:2288	acute allergic symptoms	2266:2288	acute allergic symptoms induced by OIT+FOS	2266:2307	Future research should confirm whether there is a causal relationship between microbial modulation and the reduction in acute allergic symptoms induced by OIT+FOS.					
32066258	7	64	theme	cecum	1035:1039	arg1	content					1041:1047	cecum content	1035:1047	cecum content	1035:1047	SCFA concentrations were determined in cecum content.					
32066258	1	65	theme	cell	359:362	arg1	degranulation					364:376	mast cell degranulation	354:376	mast cell degranulation	354:376	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	15	66	theme	causal	2196:2201	arg1	relationship					2203:2214	a causal relationship	2194:2214	a causal relationship between microbial modulation	2194:2243	Future research should confirm whether there is a causal relationship between microbial modulation and the reduction in acute allergic symptoms induced by OIT+FOS.					
32066258	1	67	dep	fructo-oligosaccharides	216:238	arg1	FOS					241:243	FOS	241:243	FOS	241:243	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	1	67	dep	fructo-oligosaccharides	216:238	arg1	prebiotics					246:255	prebiotics	246:255	prebiotics	246:255	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	1	68	theme	mice	299:302	arg1	diet					271:274	the diet	267:274	the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation	267:376	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	2	69	dep	metabolites	478:488	arg1	SCFA					521:524	SCFA	521:524	SCFA	521:524	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	2	69	dep	metabolites	478:488	arg1	acids					514:518	short-chain fatty acids	496:518	short-chain fatty acids (SCFA)	496:525	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	7	70	theme	SCFA	996:999	arg1	concentrations					1001:1014	SCFA concentrations	996:1014	SCFA concentrations	996:1014	SCFA concentrations were determined in cecum content.					
32066258	6	71	theme	faecal	901:906	arg1	samples					908:914	faecal samples	901:914	faecal samples	901:914	After three weeks, faecal samples were collected to analyse gut microbiota composition using 16S rRNA sequencing.					
32066258	13	72	theme	increased	1951:1959	arg1	concentrations					1993:2006	increased butyric acid and propionic acid concentrations	1951:2006	increased butyric acid and propionic acid concentrations	1951:2006	OIT+FOS mice had increased butyric acid and propionic acid concentrations.					
32066258	9	73	theme	high	1231:1234	arg1	level					1236:1240	a high level	1229:1240	a high level of resemblance in bacterial community structure	1229:1288	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	13	74	theme	acid	1969:1972	arg1	concentrations					1993:2006	increased butyric acid and propionic acid concentrations	1951:2006	increased butyric acid and propionic acid concentrations	1951:2006	OIT+FOS mice had increased butyric acid and propionic acid concentrations.					
32066258	0	75	theme	milk	62:65	arg1	mice					76:79	cow's milk allergic mice	56:79	cow's milk allergic mice	56:79	Altered microbial community structure and metabolism in cow's milk allergic mice treated with oral immunotherapy and fructo-oligosaccharides.					
32066258	15	76	theme	allergic	2272:2279	arg1	symptoms					2281:2288	acute allergic symptoms	2266:2288	acute allergic symptoms induced by OIT+FOS	2266:2307	Future research should confirm whether there is a causal relationship between microbial modulation and the reduction in acute allergic symptoms induced by OIT+FOS.					
32066258	2	77	theme	metabolites	478:488	arg1	availability					462:473	availability	462:473	availability	462:473	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	2	77	theme	metabolites	478:488	arg1	composition					446:456	bacterial composition	436:456	bacterial composition	436:456	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	0	78	from	structure	28:36	arg1	mice					76:79	cow's milk allergic mice	56:79	cow's milk allergic mice	56:79	Altered microbial community structure and metabolism in cow's milk allergic mice treated with oral immunotherapy and fructo-oligosaccharides.					
32066258	12	79	theme	beneficial	1896:1905	arg1	bacteria					1907:1914	putative beneficial bacteria	1887:1914	putative beneficial bacteria	1887:1914	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	4	80	dep	composition	736:746	arg1	the					721:723	the	721:723	the	721:723	To explore potential changes in the microbiota composition and availability of SCFA induced by OIT+FOS.					
32066258	6	81	theme	16S	975:977	arg1	sequencing					984:993	16S rRNA sequencing	975:993	16S rRNA sequencing	975:993	After three weeks, faecal samples were collected to analyse gut microbiota composition using 16S rRNA sequencing.					
32066258	11	82	theme	FOS	1501:1503	arg1	supplementation					1505:1519	FOS supplementation	1501:1519	FOS supplementation	1501:1519	FOS supplementation increased the relative abundance of genus Allobaculum (Firmicutes), putative butyrate-producing bacteria.					
32066258	9	83	theme	community	1270:1278	arg1	structure					1280:1288	bacterial community structure	1260:1288	bacterial community structure	1260:1288	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	12	84	theme	putative	1717:1724	arg1	Rikenellaceae					1687:1699	the genera's unclassified Rikenellaceae	1661:1699	the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria)	1661:1751	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	12	84	theme	putative	1717:1724	arg1	bacteria					1743:1750	putative pro-inflammatory bacteria	1717:1750	putative pro-inflammatory bacteria	1717:1750	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	2	85	theme	fatty	508:512	arg1	SCFA					521:524	SCFA	521:524	SCFA	521:524	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	2	85	theme	fatty	508:512	arg1	acids					514:518	short-chain fatty acids	496:518	short-chain fatty acids (SCFA)	496:525	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	13	86	contain	had	1947:1949	arg1	mice					1942:1945	OIT+FOS mice	1934:1945	OIT+FOS mice	1934:1945	OIT+FOS mice had increased butyric acid and propionic acid concentrations.					
32066258	13	86	contain	had	1947:1949	arg2	concentrations					1993:2006	increased butyric acid and propionic acid concentrations	1951:2006	increased butyric acid and propionic acid concentrations	1951:2006	OIT+FOS mice had increased butyric acid and propionic acid concentrations.					
32066258	4	87	theme	microbiota	725:734	arg1	composition					736:746	microbiota composition	725:746	microbiota composition	725:746	To explore potential changes in the microbiota composition and availability of SCFA induced by OIT+FOS.					
32066258	1	88	theme	clinical	332:339	arg1	symptoms					341:348	clinical symptoms	332:348	clinical symptoms	332:348	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	0	89	from	metabolism	42:51	arg1	mice					76:79	cow's milk allergic mice	56:79	cow's milk allergic mice	56:79	Altered microbial community structure and metabolism in cow's milk allergic mice treated with oral immunotherapy and fructo-oligosaccharides.					
32066258	0	90	theme	microbial	8:16	arg1	structure					28:36	Altered microbial community structure	0:36	Altered microbial community structure	0:36	Altered microbial community structure and metabolism in cow's milk allergic mice treated with oral immunotherapy and fructo-oligosaccharides.					
32066258	8	91	theme	FOS	1050:1052	arg1	supplementation					1054:1068	FOS supplementation	1050:1068	FOS supplementation in sensitised mice	1050:1087	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	10	92	theme	phylum	1449:1454	arg1	Proteobacteria					1456:1469	the dysbiosis-associated phylum Proteobacteria	1424:1469	the dysbiosis-associated phylum Proteobacteria	1424:1469	OIT mice showed an increased relative abundance of the dysbiosis-associated phylum Proteobacteria compared to the OIT+FOS mice.					
32066258	4	93	theme	potential	700:708	arg1	changes					710:716	potential changes	700:716	potential changes in the microbiota composition and availability of SCFA induced by OIT+FOS	700:790	To explore potential changes in the microbiota composition and availability of SCFA induced by OIT+FOS.					
32066258	12	94	dep	Firmicutes	1875:1884	arg1	bacteria					1907:1914	putative beneficial bacteria	1887:1914	putative beneficial bacteria	1887:1914	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	5	95	theme	C3H/HeOuJ	793:801	arg1	mice					803:806	C3H/HeOuJ mice	793:806	C3H/HeOuJ mice	793:806	C3H/HeOuJ mice were sensitised and received OIT with or without a FOS supplemented diet.					
32066258	9	96	theme	non-sensitised	1315:1328	arg1	mice					1338:1341	the non-sensitised control mice	1311:1341	the non-sensitised control mice	1311:1341	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	2	97	theme	gut	407:409	arg1	bacteria					411:418	gut bacteria	407:418	gut bacteria	407:418	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	14	98	theme	acid	2140:2143	arg1	concentrations					2095:2108	concentrations	2095:2108	concentrations of butyric acid and propionic acid	2095:2143	OIT+FOS induced a microbial profile closely linked to non-allergic mice and increased concentrations of butyric acid and propionic acid.					
32066258	2	99	dep	acids	514:518	arg1	i.e.					491:494	i.e.	491:494	i.e.	491:494	Prebiotics are fermented by gut bacteria, affecting both bacterial composition and availability of metabolites (i.e. short-chain fatty acids (SCFA)).					
32066258	14	100	theme	microbial	2027:2035	arg1	profile					2037:2043	a microbial profile	2025:2043	a microbial profile closely linked to non-allergic mice	2025:2079	OIT+FOS induced a microbial profile closely linked to non-allergic mice and increased concentrations of butyric acid and propionic acid.					
32066258	3	101	theme	successful	596:605	arg1	outcomes					607:614	successful outcomes	596:614	successful outcomes of OIT with prebiotic supplementation for the treatment of food allergy	596:686	It is thus far unknown which microbial alterations are involved in successful outcomes of OIT with prebiotic supplementation for the treatment of food allergy.					
32066258	11	102	theme	butyrate-producing	1598:1615	arg1	bacteria					1617:1624	putative butyrate-producing bacteria	1589:1624	putative butyrate-producing bacteria	1589:1624	FOS supplementation increased the relative abundance of genus Allobaculum (Firmicutes), putative butyrate-producing bacteria.					
32066258	11	102	theme	butyrate-producing	1598:1615	arg1	Allobaculum					1563:1573	genus Allobaculum	1557:1573	genus Allobaculum (Firmicutes)	1557:1586	FOS supplementation increased the relative abundance of genus Allobaculum (Firmicutes), putative butyrate-producing bacteria.					
32066258	1	103	theme	milk	285:288	arg1	mice					299:302	cow's milk allergic mice	279:302	cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation	279:376	Previously, we showed enhanced efficacy of oral immunotherapy (OIT) using fructo-oligosaccharides (FOS, prebiotics) added to the diet of cow's milk allergic mice indicated by a reduction in clinical symptoms and mast cell degranulation.					
32066258	12	104	theme	putative	1887:1894	arg1	bacteria					1907:1914	putative beneficial bacteria	1887:1914	putative beneficial bacteria	1887:1914	OIT+FOS reduced the abundances of the genera's unclassified Rikenellaceae (Bacteroidetes, putative pro-inflammatory bacteria) and unclassified Clostridiales (Firmicutes) compared to sensitised controls and increased the abundance of Lactobacillus (Firmicutes, putative beneficial bacteria) compared to FOS.					
32066258	14	105	theme	butyric	2113:2119	arg1	acid					2121:2124	butyric acid	2113:2124	butyric acid	2113:2124	OIT+FOS induced a microbial profile closely linked to non-allergic mice and increased concentrations of butyric acid and propionic acid.					
32066258	9	106	theme	treated	1359:1365	arg1	mice					1367:1370	the OIT+FOS treated mice	1347:1370	the OIT+FOS treated mice	1347:1370	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	8	107	theme	microbial	1109:1117	arg1	structure					1129:1137	the overall microbial community structure	1097:1137	the overall microbial community structure in faecal samples	1097:1155	FOS supplementation in sensitised mice changed the overall microbial community structure in faecal samples compared to sensitised mice fed the control diet (P=0.03).					
32066258	9	108	from	level	1236:1240	arg1	structure					1280:1288	bacterial community structure	1260:1288	bacterial community structure	1260:1288	In contrast, a high level of resemblance in bacterial community structure was observed between the non-sensitised control mice and the OIT+FOS treated mice.					
32066258	15	109	theme	Future	2146:2151	arg1	research					2153:2160	Future research	2146:2160	Future research	2146:2160	Future research should confirm whether there is a causal relationship between microbial modulation and the reduction in acute allergic symptoms induced by OIT+FOS.					
29459999	2	0	theme	free	342:345	arg1	sugars					347:352	free sugars	342:352	free sugars	342:352	The pretreatment of stalks without the extraction of free sugars is more complicated than typical lignocelluloses, because of the degradation of free sugars during most pretreatment processes.					
29459999	8	1	dep	liquor	1419:1424	arg1	the					1415:1417	the	1415:1417	the	1415:1417	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	9	2	theme	gasoline	1576:1583	arg1	equivalent					1585:1594	the highest gasoline equivalent	1564:1594	the highest gasoline equivalent of 0.249 L/kg	1564:1608	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	7	3	theme	water	1085:1089	arg1	ratio					1091:1095	alcohol to water ratio	1074:1095	alcohol to water ratio	1074:1095	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	7	4	theme	alcohol	1074:1080	arg1	ratio					1091:1095	alcohol to water ratio	1074:1095	alcohol to water ratio	1074:1095	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	1	5	theme	biofuel	177:183	arg1	production					185:194	biofuel production	177:194	biofuel production	177:194	Besides free sugars, sweet sorghum stalks contain cellulose and hemicellulose that can be used for biofuel production.					
29459999	2	6	theme	typical	287:293	arg1	lignocelluloses					295:309	typical lignocelluloses	287:309	typical lignocelluloses	287:309	The pretreatment of stalks without the extraction of free sugars is more complicated than typical lignocelluloses, because of the degradation of free sugars during most pretreatment processes.					
29459999	8	7	theme	0.170	1360:1364	arg1	equivalent					1346:1355	the gasoline equivalent	1333:1355	the gasoline equivalent of 0.170 and 0.241 L/kg, respectively	1333:1393	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	5	8	theme	solid	806:810	arg1	fraction					812:819	a highly degradable solid fraction	786:819	a highly degradable solid fraction	786:819	The process was optimized to obtain a liquor containing free sugars with the least sugar degradations together with a highly degradable solid fraction.					
29459999	7	9	theme	most	1007:1010	arg1	parameters					1037:1046	The most influencing pretreatment parameters	1003:1046	The most influencing pretreatment parameters	1003:1046	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	6	10	theme	ethanol	926:932	arg1	production					934:943	ethanol production	926:943	ethanol production via simultaneous saccharification and fermentation (SSF)	926:1000	The liquor was subjected to anaerobic digestion for biomethane production, while the solid was used for ethanol production via simultaneous saccharification and fermentation (SSF).					
29459999	8	11	theme	0.241 L/kg	1370:1379	arg1	equivalent					1346:1355	the gasoline equivalent	1333:1355	the gasoline equivalent of 0.170 and 0.241 L/kg, respectively	1333:1393	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	2	12	theme	free	250:253	arg1	sugars					255:260	free sugars	250:260	free sugars	250:260	The pretreatment of stalks without the extraction of free sugars is more complicated than typical lignocelluloses, because of the degradation of free sugars during most pretreatment processes.					
29459999	1	13	used	used	168:171	arg2	hemicellulose					142:154	hemicellulose	142:154	hemicellulose	142:154	Besides free sugars, sweet sorghum stalks contain cellulose and hemicellulose that can be used for biofuel production.					
29459999	1	13	used	used	168:171	arg2	cellulose					128:136	cellulose	128:136	cellulose	128:136	Besides free sugars, sweet sorghum stalks contain cellulose and hemicellulose that can be used for biofuel production.					
29459999	8	14	theme	methane	1254:1260	arg1	yields					1285:1290	The maximum methane and ethanol production yields	1242:1290	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively)	1242:1394	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	10	15	theme	pretreatment	1929:1940	arg1	effects					1897:1903	the effects	1893:1903	the effects of this newly developed pretreatment on the substrate	1893:1957	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	0	16	theme	biorefinery	53:63	arg1	perspective					65:75	a biorefinery perspective	51:75	a biorefinery perspective	51:75	Bioenergy production from sweet sorghum stalks via a biorefinery perspective.					
29459999	8	17	theme	ethanol	1266:1272	arg1	production					1274:1283	ethanol production	1266:1283	ethanol production	1266:1283	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	5	18	theme	sugar	753:757	arg1	degradations					759:770	the least sugar degradations	743:770	the least sugar degradations together with a highly degradable solid fraction	743:819	The process was optimized to obtain a liquor containing free sugars with the least sugar degradations together with a highly degradable solid fraction.					
29459999	9	19	theme	60:20	1704:1708	arg1	ratio					1720:1724	60:20 EtOH/IPOH ratio	1704:1724	60:20 EtOH/IPOH ratio	1704:1724	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	8	20	theme	271.2 mL	1295:1302	arg1	CH4/g					1304:1308	271.2 mL CH4/g	1295:1308	271.2 mL CH4/g	1295:1308	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	3	21	theme	sweet	426:430	arg1	stalks					440:445	sweet sorghum stalks	426:445	sweet sorghum stalks	426:445	In this study, the bioconversion of sweet sorghum stalks into biogas and bioethanol was studied using an improved organosolv pretreatment within a biorefinery framework.					
29459999	7	22	theme	sulfuric	1149:1156	arg1	acid					1158:1161	sulfuric acid	1149:1161	sulfuric acid	1149:1161	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	10	23	from	effects	1897:1903	arg1	substrate					1949:1957	the substrate	1945:1957	the substrate	1945:1957	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	5	24	theme	degradable	795:804	arg1	fraction					812:819	a highly degradable solid fraction	786:819	a highly degradable solid fraction	786:819	The process was optimized to obtain a liquor containing free sugars with the least sugar degradations together with a highly degradable solid fraction.					
29459999	1	25	theme	sweet	99:103	arg1	stalks					113:118	sweet sorghum stalks	99:118	sweet sorghum stalks	99:118	Besides free sugars, sweet sorghum stalks contain cellulose and hemicellulose that can be used for biofuel production.					
29459999	9	26	theme	%	1746:1746	arg1	acid					1757:1760	1% sulfuric acid	1745:1760	1% sulfuric acid	1745:1760	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	4	27	theme	organosolv	564:573	arg1	pretreatment					575:586	The organosolv pretreatment	560:586	The organosolv pretreatment	560:586	The organosolv pretreatment was developed using an aqueous solution of ethanol (EtOH) and isopropanol (IPOH).					
29459999	3	28	theme	stalks	440:445	arg1	bioconversion					409:421	the bioconversion	405:421	the bioconversion of sweet sorghum stalks into biogas and bioethanol	405:472	In this study, the bioconversion of sweet sorghum stalks into biogas and bioethanol was studied using an improved organosolv pretreatment within a biorefinery framework.					
29459999	0	29	theme	sorghum	32:38	arg1	stalks					40:45	sweet sorghum stalks	26:45	sweet sorghum stalks	26:45	Bioenergy production from sweet sorghum stalks via a biorefinery perspective.					
29459999	7	30	theme	bioconversion	1227:1239	arg1	yields					1217:1222	the highest yields	1205:1222	the highest yields of bioconversion	1205:1239	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	9	31	theme	acid	1757:1760	arg1	presence					1733:1740	the presence	1729:1740	the presence of 1% sulfuric acid	1729:1760	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	10	32	theme	Further	1763:1769	arg1	FTIR					1848:1851	FTIR	1848:1851	FTIR	1848:1851	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	10	32	theme	Further	1763:1769	arg1	adsorption/desorption					1801:1821	enzymatic adsorption/desorption	1791:1821	enzymatic adsorption/desorption	1791:1821	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	10	32	theme	Further	1763:1769	arg1	analysis					1838:1845	compositional analysis	1824:1845	compositional analysis	1824:1845	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	10	32	theme	Further	1763:1769	arg1	analyses					1771:1778	Further analyses	1763:1778	Further analyses	1763:1778	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	10	32	theme	Further	1763:1769	arg1	SEM					1858:1860	SEM	1858:1860	SEM	1858:1860	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	8	33	theme	gasoline	1337:1344	arg1	equivalent					1346:1355	the gasoline equivalent	1333:1355	the gasoline equivalent of 0.170 and 0.241 L/kg, respectively	1333:1393	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	9	34	from	140 °C	1680:1685	arg1	pretreatment					1664:1675	pretreatment	1664:1675	pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid	1664:1760	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	9	35	theme	biorefinery	1539:1549	arg1	perspective					1551:1561	the biorefinery perspective	1535:1561	the biorefinery perspective	1535:1561	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	8	36	theme	optimum	1503:1509	arg1	conditions					1511:1520	different optimum conditions	1493:1520	different optimum conditions	1493:1520	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	10	37	theme	enzymatic	1791:1799	arg1	adsorption/desorption					1801:1821	enzymatic adsorption/desorption	1791:1821	enzymatic adsorption/desorption	1791:1821	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	3	38	theme	organosolv	504:513	arg1	pretreatment					515:526	an improved organosolv pretreatment	492:526	an improved organosolv pretreatment	492:526	In this study, the bioconversion of sweet sorghum stalks into biogas and bioethanol was studied using an improved organosolv pretreatment within a biorefinery framework.					
29459999	1	39	contain	contain	120:126	arg2	hemicellulose					142:154	hemicellulose	142:154	hemicellulose	142:154	Besides free sugars, sweet sorghum stalks contain cellulose and hemicellulose that can be used for biofuel production.					
29459999	1	39	contain	contain	120:126	arg2	cellulose					128:136	cellulose	128:136	cellulose	128:136	Besides free sugars, sweet sorghum stalks contain cellulose and hemicellulose that can be used for biofuel production.					
29459999	1	39	contain	contain	120:126	arg1	stalks					113:118	sweet sorghum stalks	99:118	sweet sorghum stalks	99:118	Besides free sugars, sweet sorghum stalks contain cellulose and hemicellulose that can be used for biofuel production.					
29459999	7	40	theme	EtOH	1098:1101	arg1	ratio					1111:1115	EtOH to IPOH ratio	1098:1115	EtOH to IPOH ratio	1098:1115	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	2	41	theme	sugars	347:352	arg1	degradation					327:337	the degradation	323:337	the degradation of free sugars during most pretreatment processes	323:387	The pretreatment of stalks without the extraction of free sugars is more complicated than typical lignocelluloses, because of the degradation of free sugars during most pretreatment processes.					
29459999	9	42	theme	highest	1568:1574	arg1	equivalent					1585:1594	the highest gasoline equivalent	1564:1594	the highest gasoline equivalent of 0.249 L/kg	1564:1608	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	3	43	theme	biorefinery	537:547	arg1	framework					549:557	a biorefinery framework	535:557	a biorefinery framework	535:557	In this study, the bioconversion of sweet sorghum stalks into biogas and bioethanol was studied using an improved organosolv pretreatment within a biorefinery framework.					
29459999	6	44	theme	biomethane	874:883	arg1	production					885:894	biomethane production	874:894	biomethane production	874:894	The liquor was subjected to anaerobic digestion for biomethane production, while the solid was used for ethanol production via simultaneous saccharification and fermentation (SSF).					
29459999	9	45	theme	0.249 L/kg	1599:1608	arg1	equivalent					1585:1594	the highest gasoline equivalent	1564:1594	the highest gasoline equivalent of 0.249 L/kg	1564:1608	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	4	46	theme	isopropanol	650:660	arg1	solution					619:626	an aqueous solution	608:626	an aqueous solution of ethanol (EtOH) and isopropanol (IPOH)	608:667	The organosolv pretreatment was developed using an aqueous solution of ethanol (EtOH) and isopropanol (IPOH).					
29459999	7	47	dep	ratio	1091:1095	arg1	to					1082:1083	to	1082:1083	to	1082:1083	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	6	48	used	used	917:920	arg2	solid					907:911	solid	907:911	solid	907:911	The liquor was subjected to anaerobic digestion for biomethane production, while the solid was used for ethanol production via simultaneous saccharification and fermentation (SSF).					
29459999	6	49	theme	simultaneous	949:960	arg1	saccharification					962:977	simultaneous saccharification	949:977	simultaneous saccharification	949:977	The liquor was subjected to anaerobic digestion for biomethane production, while the solid was used for ethanol production via simultaneous saccharification and fermentation (SSF).					
29459999	9	50	theme	whole	1644:1648	arg1	process					1650:1656	the whole process	1640:1656	the whole process	1640:1656	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	2	51	theme	stalks	217:222	arg1	complicated					270:280	complicated	270:280	complicated	270:280	The pretreatment of stalks without the extraction of free sugars is more complicated than typical lignocelluloses, because of the degradation of free sugars during most pretreatment processes.					
29459999	2	51	theme	stalks	217:222	arg1	pretreatment					201:212	The pretreatment	197:212	The pretreatment of stalks without the extraction of free sugars	197:260	The pretreatment of stalks without the extraction of free sugars is more complicated than typical lignocelluloses, because of the degradation of free sugars during most pretreatment processes.					
29459999	7	52	theme	influencing	1012:1022	arg1	parameters					1037:1046	The most influencing pretreatment parameters	1003:1046	The most influencing pretreatment parameters	1003:1046	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	1	53	theme	sorghum	105:111	arg1	stalks					113:118	sweet sorghum stalks	99:118	sweet sorghum stalks	99:118	Besides free sugars, sweet sorghum stalks contain cellulose and hemicellulose that can be used for biofuel production.					
29459999	7	54	theme	pretreatment	1024:1035	arg1	parameters					1037:1046	The most influencing pretreatment parameters	1003:1046	The most influencing pretreatment parameters	1003:1046	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	2	55	theme	sugars	255:260	arg1	extraction					236:245	the extraction	232:245	the extraction of free sugars	232:260	The pretreatment of stalks without the extraction of free sugars is more complicated than typical lignocelluloses, because of the degradation of free sugars during most pretreatment processes.					
29459999	8	56	theme	maximum	1246:1252	arg1	methane					1254:1260	The maximum methane	1242:1260	The maximum methane	1242:1260	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	10	57	theme	developed	1919:1927	arg1	pretreatment					1929:1940	this newly developed pretreatment	1908:1940	this newly developed pretreatment	1908:1940	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	5	58	theme	least	747:751	arg1	degradations					759:770	the least sugar degradations	743:770	the least sugar degradations together with a highly degradable solid fraction	743:819	The process was optimized to obtain a liquor containing free sugars with the least sugar degradations together with a highly degradable solid fraction.					
29459999	7	59	dep	presence	1126:1133	arg1	the					1122:1124	the	1122:1124	the	1122:1124	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	7	60	theme	acid	1158:1161	arg1	presence					1126:1133	presence	1126:1133	presence	1126:1133	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	7	60	theme	acid	1158:1161	arg1	absence					1138:1144	absence	1138:1144	absence	1138:1144	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	7	60	theme	acid	1158:1161	arg1	ratio					1111:1115	EtOH to IPOH ratio	1098:1115	EtOH to IPOH ratio	1098:1115	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	7	60	theme	acid	1158:1161	arg1	temperature					1055:1065	temperature	1055:1065	temperature	1055:1065	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	7	60	theme	acid	1158:1161	arg1	ratio					1091:1095	alcohol to water ratio	1074:1095	alcohol to water ratio	1074:1095	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	7	60	theme	acid	1158:1161	arg1	time					1068:1071	time	1068:1071	time	1068:1071	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	9	61	theme	EtOH/IPOH	1710:1718	arg1	ratio					1720:1724	60:20 EtOH/IPOH ratio	1704:1724	60:20 EtOH/IPOH ratio	1704:1724	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	8	62	theme	production	1274:1283	arg1	yields					1285:1290	The maximum methane and ethanol production yields	1242:1290	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively)	1242:1394	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	5	63	theme	free	726:729	arg1	sugars					731:736	free sugars	726:736	free sugars	726:736	The process was optimized to obtain a liquor containing free sugars with the least sugar degradations together with a highly degradable solid fraction.					
29459999	0	64	from	stalks	40:45	arg1	production					10:19	production	10:19	production from sweet sorghum stalks	10:45	Bioenergy production from sweet sorghum stalks via a biorefinery perspective.					
29459999	7	65	dep	ratio	1111:1115	arg1	to					1103:1104	to	1103:1104	to	1103:1104	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	4	66	theme	aqueous	611:617	arg1	solution					619:626	an aqueous solution	608:626	an aqueous solution of ethanol (EtOH) and isopropanol (IPOH)	608:667	The organosolv pretreatment was developed using an aqueous solution of ethanol (EtOH) and isopropanol (IPOH).					
29459999	3	67	theme	sorghum	432:438	arg1	stalks					440:445	sweet sorghum stalks	426:445	sweet sorghum stalks	426:445	In this study, the bioconversion of sweet sorghum stalks into biogas and bioethanol was studied using an improved organosolv pretreatment within a biorefinery framework.					
29459999	1	68	theme	free	86:89	arg1	sugars					91:96	free sugars	86:96	free sugars	86:96	Besides free sugars, sweet sorghum stalks contain cellulose and hemicellulose that can be used for biofuel production.					
29459999	8	69	theme	CH4/g	1304:1308	arg1	yields					1285:1290	The maximum methane and ethanol production yields	1242:1290	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively)	1242:1394	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	2	70	theme	pretreatment	366:377	arg1	processes					379:387	most pretreatment processes	361:387	most pretreatment processes	361:387	The pretreatment of stalks without the extraction of free sugars is more complicated than typical lignocelluloses, because of the degradation of free sugars during most pretreatment processes.					
29459999	0	71	theme	sweet	26:30	arg1	stalks					40:45	sweet sorghum stalks	26:45	sweet sorghum stalks	26:45	Bioenergy production from sweet sorghum stalks via a biorefinery perspective.					
29459999	9	72	theme	1	1745:1745	arg1	%					1746:1746	%	1746:1746	%	1746:1746	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	2	73	theme	most	361:364	arg1	processes					379:387	most pretreatment processes	361:387	most pretreatment processes	361:387	The pretreatment of stalks without the extraction of free sugars is more complicated than typical lignocelluloses, because of the degradation of free sugars during most pretreatment processes.					
29459999	6	74	theme	anaerobic	850:858	arg1	digestion					860:868	anaerobic digestion	850:868	anaerobic digestion	850:868	The liquor was subjected to anaerobic digestion for biomethane production, while the solid was used for ethanol production via simultaneous saccharification and fermentation (SSF).					
29459999	9	75	theme	sulfuric	1748:1755	arg1	acid					1757:1760	1% sulfuric acid	1745:1760	1% sulfuric acid	1745:1760	Considering the biorefinery perspective, the highest gasoline equivalent of 0.249 L/kg was efficiently obtained from the whole process after pretreatment at 140 °C for 30 min using 60:20 EtOH/IPOH ratio in the presence of 1% sulfuric acid.					
29459999	8	76	theme	%	1321:1321	arg1	yields					1285:1290	The maximum methane and ethanol production yields	1242:1290	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively)	1242:1394	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	7	77	theme	IPOH	1106:1109	arg1	ratio					1111:1115	EtOH to IPOH ratio	1098:1115	EtOH to IPOH ratio	1098:1115	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	5	78	contain	containing	715:724	arg1	liquor					708:713	a liquor	706:713	a liquor containing free sugars with the least sugar degradations together with a highly degradable solid fraction	706:819	The process was optimized to obtain a liquor containing free sugars with the least sugar degradations together with a highly degradable solid fraction.					
29459999	5	78	contain	containing	715:724	arg2	sugars					731:736	free sugars	726:736	free sugars	726:736	The process was optimized to obtain a liquor containing free sugars with the least sugar degradations together with a highly degradable solid fraction.					
29459999	7	79	theme	highest	1209:1215	arg1	yields					1217:1222	the highest yields	1205:1222	the highest yields of bioconversion	1205:1239	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29459999	8	80	theme	different	1493:1501	arg1	conditions					1511:1520	different optimum conditions	1493:1520	different optimum conditions	1493:1520	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	10	81	theme	compositional	1824:1836	arg1	analysis					1838:1845	compositional analysis	1824:1845	compositional analysis	1824:1845	Further analyses, including enzymatic adsorption/desorption, compositional analysis, FTIR, and SEM, were conducted to investigate the effects of this newly developed pretreatment on the substrate.					
29459999	8	82	theme	equal	1324:1328	arg1	CH4/g					1304:1308	271.2 mL CH4/g	1295:1308	271.2 mL CH4/g	1295:1308	The maximum methane and ethanol production yields of 271.2 mL CH4/g VS and 87.8% (equal to the gasoline equivalent of 0.170 and 0.241 L/kg, respectively) were achieved from the liquor and pretreated solid, respectively; however, they were obtained at different optimum conditions.					
29459999	3	83	theme	improved	495:502	arg1	pretreatment					515:526	an improved organosolv pretreatment	492:526	an improved organosolv pretreatment	492:526	In this study, the bioconversion of sweet sorghum stalks into biogas and bioethanol was studied using an improved organosolv pretreatment within a biorefinery framework.					
29459999	4	84	theme	ethanol	631:637	arg1	solution					619:626	an aqueous solution	608:626	an aqueous solution of ethanol (EtOH) and isopropanol (IPOH)	608:667	The organosolv pretreatment was developed using an aqueous solution of ethanol (EtOH) and isopropanol (IPOH).					
29459999	7	85	dep	temperature	1055:1065	arg1	i.e.					1049:1052	i.e.	1049:1052	i.e.	1049:1052	The most influencing pretreatment parameters, i.e., temperature, time, alcohol to water ratio, EtOH to IPOH ratio, and the presence or absence of sulfuric acid (as a catalyst), were adjusted to achieve the highest yields of bioconversion.					
29886256	6	0	theme	monodispersed	1067:1079	arg1	droplets					1081:1088	the generated monodispersed droplets	1053:1088	the generated monodispersed droplets	1053:1088	A novelty of the work reported here is that the size of the generated monodispersed droplets could be precisely controlled in the range 50 μm-200 μm by varying the flow rates of the dispersed and continuous phases.					
29886256	10	1	theme	process	1865:1871	arg1	scale-up					1873:1880	process scale-up	1865:1880	process scale-up	1865:1880	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	2	2	theme	sodium	342:347	arg1	alginate					349:356	the biopolymer sodium alginate	327:356	the biopolymer sodium alginate	327:356	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	11	3	theme	microparticles	2029:2042	arg1	exposure					2006:2013	exposure	2006:2013	exposure of the gelled microparticles to SGF (pH 1 for 2 h)	2006:2064	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	3	4	theme	microfluidic	494:505	arg1	devices					526:532	commercially available microfluidic droplet generation devices	471:532	commercially available microfluidic droplet generation devices	471:532	The microencapsulation process employed commercially available microfluidic droplet generation devices.					
29886256	13	5	theme	gastrointestinal	2573:2588	arg1	tract					2590:2594	the gastrointestinal tract	2569:2594	the gastrointestinal tract	2569:2594	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	9	6	theme	particle	1649:1656	arg1	sizes					1658:1662	similar particle sizes	1641:1662	similar particle sizes	1641:1662	Increasing the alginate composition resulted in improved acid protection of phages for similar particle sizes.					
29886256	4	7	theme	fabricated	622:631	arg1	devices					649:655	bespoke in-house fabricated glass capillary devices	605:655	bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	605:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	1	8	theme	polymer	186:192	arg1	formulation					194:204	a pH responsive polymer formulation	170:204	a pH responsive polymer formulation	170:204	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	7	9	theme	simulated	1392:1400	arg1	SGF					1417:1419	SGF	1417:1419	SGF	1417:1419	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	7	9	theme	simulated	1392:1400	arg1	fluid					1410:1414	simulated gastric fluid	1392:1414	simulated gastric fluid (SGF)	1392:1420	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	4	10	theme	capillary	639:647	arg1	devices					649:655	bespoke in-house fabricated glass capillary devices	605:655	bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	605:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	13	11	theme	available	2398:2406	arg1	chips					2421:2425	readily available microfluidic chips	2390:2425	readily available microfluidic chips	2390:2425	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	13	12	theme	controlled	2547:2556	arg1	release					2558:2564	controlled release	2547:2564	controlled release	2547:2564	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	13	13	from	release	2558:2564	arg1	tract					2590:2594	the gastrointestinal tract	2569:2594	the gastrointestinal tract	2569:2594	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	6	14	theme	range	1127:1131	arg1	50 μm-200 μm					1133:1144	the range 50 μm-200 μm	1123:1144	the range 50 μm-200 μm	1123:1144	A novelty of the work reported here is that the size of the generated monodispersed droplets could be precisely controlled in the range 50 μm-200 μm by varying the flow rates of the dispersed and continuous phases.					
29886256	6	15	theme	generated	1057:1065	arg1	droplets					1081:1088	the generated monodispersed droplets	1053:1088	the generated monodispersed droplets	1053:1088	A novelty of the work reported here is that the size of the generated monodispersed droplets could be precisely controlled in the range 50 μm-200 μm by varying the flow rates of the dispersed and continuous phases.					
29886256	12	16	theme	elevated	2205:2212	arg1	pH					2214:2215	elevated pH	2205:2215	elevated pH	2205:2215	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	4	17	theme	bespoke	605:611	arg1	devices					649:655	bespoke in-house fabricated glass capillary devices	605:655	bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	605:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	2	18	theme	methacrylic	252:262	arg1	S100					289:292	a pH responsive methacrylic acid copolymer Eudragit® S100	236:292	a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v))	236:304	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	12	19	theme	simulated	2220:2228	arg1	SIF					2248:2250	SIF	2248:2250	SIF	2248:2250	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	12	19	theme	simulated	2220:2228	arg1	fluid					2241:2245	simulated intestinal fluid	2220:2245	simulated intestinal fluid (SIF)	2220:2251	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	6	20	theme	phases	1204:1209	arg1	rates					1166:1170	the flow rates	1157:1170	the flow rates of the dispersed and continuous phases	1157:1209	A novelty of the work reported here is that the size of the generated monodispersed droplets could be precisely controlled in the range 50 μm-200 μm by varying the flow rates of the dispersed and continuous phases.					
29886256	7	21	theme	acid	1340:1343	arg1	protection					1345:1354	acid protection	1340:1354	acid protection afforded to phages upon exposure to simulated gastric fluid (SGF)	1340:1420	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	4	22	theme	research	697:704	arg1	facilities					706:715	specialist research facilities	686:715	specialist research facilities	686:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	5	23	theme	phage	796:800	arg1	microparticles					815:828	phage encapsulated microparticles	796:828	phage encapsulated microparticles	796:828	We show that these co-flow microfluidic devices can easily be used to prepare phage encapsulated microparticles making them suitable for use by both the phage research community and industry in order to evaluate and optimise phage compatible formulations for microencapsulation.					
29886256	13	24	theme	targeted	2525:2532	arg1	delivery					2534:2541	delivery	2534:2541	delivery	2534:2541	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	10	25	theme	droplet	1779:1785	arg1	generation					1787:1796	droplet generation	1779:1796	droplet generation	1779:1796	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	13	26	theme	pH	2483:2484	arg1	profile					2504:2510	a rapid pH triggered release profile	2475:2510	a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract	2475:2594	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	8	27	theme	acid	1469:1472	arg1	protection					1474:1483	better acid protection	1462:1483	better acid protection	1462:1483	Bigger microparticles (∼100 μm) showed better acid protection compared with smaller beads (∼50 μm) made from the same formulation.					
29886256	10	28	dep	%	1742:1742	arg1	e.g.					1736:1739	e.g.	1736:1739	e.g.	1736:1739	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	5	29	theme	microfluidic	745:756	arg1	devices					758:764	these co-flow microfluidic devices	731:764	these co-flow microfluidic devices	731:764	We show that these co-flow microfluidic devices can easily be used to prepare phage encapsulated microparticles making them suitable for use by both the phage research community and industry in order to evaluate and optimise phage compatible formulations for microencapsulation.					
29886256	11	30	theme	%	1937:1937	arg1	alginate					1964:1971	10% (w/v) ES100 and 1% (w/v) alginate	1935:1971	10% (w/v) ES100 and 1% (w/v) alginate	1935:1971	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	5	31	theme	research	877:884	arg1	community					886:894	the phage research community	867:894	the phage research community	867:894	We show that these co-flow microfluidic devices can easily be used to prepare phage encapsulated microparticles making them suitable for use by both the phage research community and industry in order to evaluate and optimise phage compatible formulations for microencapsulation.					
29886256	7	32	theme	protection	1345:1354	arg1	profile					1314:1320	the phage release profile	1296:1320	the phage release profile	1296:1320	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	7	32	theme	protection	1345:1354	arg1	degree					1330:1335	the degree	1326:1335	the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF)	1326:1420	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	10	33	theme	higher	1708:1713	arg1	amounts					1715:1721	higher amounts	1708:1721	higher amounts of alginate (e.g. 2% (w/v))	1708:1749	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	10	33	theme	higher	1708:1713	arg1	alginate					1726:1733	alginate	1726:1733	alginate	1726:1733	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	10	34	dep	amounts	1715:1721	arg1	w/v					1745:1747	w/v	1745:1747	w/v	1745:1747	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	10	34	dep	amounts	1715:1721	arg1	%					1742:1742	2%	1741:1742	2% (w/v)	1741:1748	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	13	35	theme	release	2496:2502	arg1	profile					2504:2510	a rapid pH triggered release profile	2475:2510	a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract	2475:2594	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	5	36	theme	phage	943:947	arg1	formulations					960:971	phage compatible formulations	943:971	phage compatible formulations for microencapsulation	943:994	We show that these co-flow microfluidic devices can easily be used to prepare phage encapsulated microparticles making them suitable for use by both the phage research community and industry in order to evaluate and optimise phage compatible formulations for microencapsulation.					
29886256	8	37	theme	smaller	1499:1505	arg1	beads					1507:1511	smaller beads	1499:1511	smaller beads (∼50 μm) made from the same formulation	1499:1551	Bigger microparticles (∼100 μm) showed better acid protection compared with smaller beads (∼50 μm) made from the same formulation.					
29886256	8	37	theme	smaller	1499:1505	arg1	∼50 μm					1514:1519	∼50 μm	1514:1519	∼50 μm	1514:1519	Bigger microparticles (∼100 μm) showed better acid protection compared with smaller beads (∼50 μm) made from the same formulation.					
29886256	13	38	theme	suitable	2512:2519	arg1	profile					2504:2510	a rapid pH triggered release profile	2475:2510	a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract	2475:2594	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	13	39	theme	solid	2438:2442	arg1	forms					2464:2468	solid dosage microcapsule forms	2438:2468	solid dosage microcapsule forms	2438:2468	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	1	40	theme	Salmonella	89:98	arg1	Myoviridae					133:142	Myoviridae	133:142	Myoviridae	133:142	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	1	40	theme	Salmonella	89:98	arg1	O1					129:130	A Salmonella specific bacteriophage Felix O1	87:130	A Salmonella specific bacteriophage Felix O1 (Myoviridae)	87:143	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	0	41	theme	High	0:3	arg1	microencapsulation					28:45	High precision microfluidic microencapsulation	0:45	High precision microfluidic microencapsulation of bacteriophages for enteric delivery.	0:85	High precision microfluidic microencapsulation of bacteriophages for enteric delivery.					
29886256	1	42	theme	bacteriophage	109:121	arg1	Myoviridae					133:142	Myoviridae	133:142	Myoviridae	133:142	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	1	42	theme	bacteriophage	109:121	arg1	O1					129:130	A Salmonella specific bacteriophage Felix O1	87:130	A Salmonella specific bacteriophage Felix O1 (Myoviridae)	87:143	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	10	43	theme	generation	1787:1796	arg1	ease					1771:1774	ease	1771:1774	ease of droplet generation in the microfluidic device	1771:1823	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	11	44	theme	excellent	1980:1988	arg1	protection					1990:1999	excellent protection	1980:1999	excellent protection	1980:1999	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	0	45	theme	microfluidic	15:26	arg1	microencapsulation					28:45	High precision microfluidic microencapsulation	0:45	High precision microfluidic microencapsulation of bacteriophages for enteric delivery.	0:85	High precision microfluidic microencapsulation of bacteriophages for enteric delivery.					
29886256	12	46	dep	SGF	2169:2171	arg1	pH					2174:2175	pH 1	2174:2177	pH 1	2174:2177	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	7	47	theme	phage	1300:1304	arg1	profile					1314:1320	the phage release profile	1296:1320	the phage release profile	1296:1320	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	11	48	theme	antacids	2089:2096	arg1	use					2078:2080	the use	2074:2080	the use of any antacids in the encapsulation matrix	2074:2124	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	12	49	theme	growth	2306:2311	arg1	phase					2313:2317	the log growth phase	2298:2317	the log growth phase	2298:2317	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	11	50	theme	Felix	1883:1887	arg1	O1					1889:1890	Felix O1	1883:1890	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate	1883:1971	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	13	51	theme	dosage	2444:2449	arg1	forms					2464:2468	solid dosage microcapsule forms	2438:2468	solid dosage microcapsule forms	2438:2468	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	9	52	theme	alginate	1569:1576	arg1	composition					1578:1588	the alginate composition	1565:1588	the alginate composition	1565:1588	Increasing the alginate composition resulted in improved acid protection of phages for similar particle sizes.					
29886256	8	53	theme	same	1536:1539	arg1	formulation					1541:1551	the same formulation	1532:1551	the same formulation	1532:1551	Bigger microparticles (∼100 μm) showed better acid protection compared with smaller beads (∼50 μm) made from the same formulation.					
29886256	10	54	theme	microfluidic	1805:1816	arg1	device					1818:1823	the microfluidic device	1801:1823	the microfluidic device	1801:1823	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	2	55	theme	biopolymer	331:340	arg1	alginate					349:356	the biopolymer sodium alginate	327:356	the biopolymer sodium alginate	327:356	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	13	56	from	delivery	2534:2541	arg1	tract					2590:2594	the gastrointestinal tract	2569:2594	the gastrointestinal tract	2569:2594	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	2	57	theme	copolymer	269:277	arg1	S100					289:292	a pH responsive methacrylic acid copolymer Eudragit® S100	236:292	a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v))	236:304	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	10	58	theme	viscosity	1674:1682	arg1	formulations					1684:1695	The high viscosity formulations	1665:1695	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v))	1665:1749	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	5	59	used	used	780:783	arg2	devices					758:764	these co-flow microfluidic devices	731:764	these co-flow microfluidic devices	731:764	We show that these co-flow microfluidic devices can easily be used to prepare phage encapsulated microparticles making them suitable for use by both the phage research community and industry in order to evaluate and optimise phage compatible formulations for microencapsulation.					
29886256	9	60	theme	improved	1602:1609	arg1	protection					1616:1625	improved acid protection	1602:1625	improved acid protection of phages for similar particle sizes	1602:1662	Increasing the alginate composition resulted in improved acid protection of phages for similar particle sizes.					
29886256	4	61	theme	available	556:564	arg1	chips					588:592	readily available low cost microfluidic chips	548:592	readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	548:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	3	62	theme	microencapsulation	435:452	arg1	process					454:460	The microencapsulation process	431:460	The microencapsulation process	431:460	The microencapsulation process employed commercially available microfluidic droplet generation devices.					
29886256	13	63	theme	microcapsule	2451:2462	arg1	forms					2464:2468	solid dosage microcapsule forms	2438:2468	solid dosage microcapsule forms	2438:2468	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	2	64	from	range	402:406	arg1	composition					363:373	the composition	359:373	the composition	359:373	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	64	from	range	402:406	arg1	varied					388:393	varied	388:393	varied	388:393	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	64	from	range	402:406	arg1	%					421:421	0.5% (w/v)-2%	409:421	0.5% (w/v)-2% (w/v)	409:427	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	6	65	theme	droplets	1081:1088	arg1	size					1045:1048	the size	1041:1048	the size of the generated monodispersed droplets	1041:1088	A novelty of the work reported here is that the size of the generated monodispersed droplets could be precisely controlled in the range 50 μm-200 μm by varying the flow rates of the dispersed and continuous phases.					
29886256	4	66	theme	cost	570:573	arg1	chips					588:592	readily available low cost microfluidic chips	548:592	readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	548:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	10	67	theme	scale-up	1873:1880	arg1	terms					1856:1860	terms	1856:1860	terms of process scale-up	1856:1880	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	13	68	theme	microfluidic	2408:2419	arg1	chips					2421:2425	readily available microfluidic chips	2390:2425	readily available microfluidic chips	2390:2425	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	2	69	theme	alginate	349:356	arg1	addition					315:322	the addition	311:322	the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v))	311:428	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	69	theme	alginate	349:356	arg1	composition					363:373	the composition	359:373	the composition	359:373	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	69	theme	alginate	349:356	arg1	varied					388:393	varied	388:393	varied	388:393	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	69	theme	alginate	349:356	arg1	%					421:421	0.5% (w/v)-2%	409:421	0.5% (w/v)-2% (w/v)	409:427	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	9	70	theme	phages	1630:1635	arg1	protection					1616:1625	improved acid protection	1602:1625	improved acid protection of phages for similar particle sizes	1602:1662	Increasing the alginate composition resulted in improved acid protection of phages for similar particle sizes.					
29886256	7	71	theme	alginate	1226:1233	arg1	concentration					1235:1247	alginate concentration	1226:1247	alginate concentration	1226:1247	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	3	72	theme	available	484:492	arg1	devices					526:532	commercially available microfluidic droplet generation devices	471:532	commercially available microfluidic droplet generation devices	471:532	The microencapsulation process employed commercially available microfluidic droplet generation devices.					
29886256	9	73	theme	similar	1641:1647	arg1	sizes					1658:1662	similar particle sizes	1641:1662	similar particle sizes	1641:1662	Increasing the alginate composition resulted in improved acid protection of phages for similar particle sizes.					
29886256	6	74	theme	flow	1161:1164	arg1	rates					1166:1170	the flow rates	1157:1170	the flow rates of the dispersed and continuous phases	1157:1209	A novelty of the work reported here is that the size of the generated monodispersed droplets could be precisely controlled in the range 50 μm-200 μm by varying the flow rates of the dispersed and continuous phases.					
29886256	13	75	theme	phages	2377:2382	arg1	microencapsulation					2355:2372	the microencapsulation	2351:2372	the microencapsulation of phages	2351:2382	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	1	76	theme	responsive	175:184	arg1	formulation					194:204	a pH responsive polymer formulation	170:204	a pH responsive polymer formulation	170:204	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	4	77	theme	in-house	613:620	arg1	devices					649:655	bespoke in-house fabricated glass capillary devices	605:655	bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	605:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	11	78	from	use	2078:2080	arg1	matrix					2119:2124	the encapsulation matrix	2101:2124	the encapsulation matrix	2101:2124	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	7	79	theme	gastric	1402:1408	arg1	SGF					1417:1419	SGF	1417:1419	SGF	1417:1419	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	7	79	theme	gastric	1402:1408	arg1	fluid					1410:1414	simulated gastric fluid	1392:1414	simulated gastric fluid (SGF)	1392:1420	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	2	80	dep	S100	289:292	arg1	w/v					300:302	w/v	300:302	w/v	300:302	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	80	dep	S100	289:292	arg1	%					297:297	10%	295:297	10% (w/v)	295:303	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	11	81	dep	SGF	2047:2049	arg1	pH					2052:2053	pH 1	2052:2055	pH 1	2052:2055	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	3	82	theme	droplet	507:513	arg1	devices					526:532	commercially available microfluidic droplet generation devices	471:532	commercially available microfluidic droplet generation devices	471:532	The microencapsulation process employed commercially available microfluidic droplet generation devices.					
29886256	5	83	theme	encapsulated	802:813	arg1	microparticles					815:828	phage encapsulated microparticles	796:828	phage encapsulated microparticles	796:828	We show that these co-flow microfluidic devices can easily be used to prepare phage encapsulated microparticles making them suitable for use by both the phage research community and industry in order to evaluate and optimise phage compatible formulations for microencapsulation.					
29886256	8	84	theme	Bigger	1423:1428	arg1	microparticles					1430:1443	Bigger microparticles	1423:1443	Bigger microparticles (∼100 μm)	1423:1453	Bigger microparticles (∼100 μm) showed better acid protection compared with smaller beads (∼50 μm) made from the same formulation.					
29886256	8	84	theme	Bigger	1423:1428	arg1	∼100 μm					1446:1452	∼100 μm	1446:1452	∼100 μm	1446:1452	Bigger microparticles (∼100 μm) showed better acid protection compared with smaller beads (∼50 μm) made from the same formulation.					
29886256	2	85	theme	acid	264:267	arg1	S100					289:292	a pH responsive methacrylic acid copolymer Eudragit® S100	236:292	a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v))	236:304	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	4	86	theme	glass	633:637	arg1	devices					649:655	bespoke in-house fabricated glass capillary devices	605:655	bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	605:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	10	87	contain	containing	1697:1706	arg1	formulations					1684:1695	The high viscosity formulations	1665:1695	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v))	1665:1749	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	10	87	contain	containing	1697:1706	arg2	alginate					1726:1733	alginate	1726:1733	alginate	1726:1733	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	10	87	contain	containing	1697:1706	arg2	amounts					1715:1721	higher amounts	1708:1721	higher amounts of alginate (e.g. 2% (w/v))	1708:1749	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	12	88	theme	intestinal	2230:2239	arg1	SIF					2248:2250	SIF	2248:2250	SIF	2248:2250	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	12	88	theme	intestinal	2230:2239	arg1	fluid					2241:2245	simulated intestinal fluid	2220:2245	simulated intestinal fluid (SIF)	2220:2251	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	4	89	used	used	543:546	arg2	We					535:536	We	535:536	We	535:536	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	2	90	theme	responsive	241:250	arg1	S100					289:292	a pH responsive methacrylic acid copolymer Eudragit® S100	236:292	a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v))	236:304	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	11	91	contain	containing	1924:1933	arg2	alginate					1964:1971	10% (w/v) ES100 and 1% (w/v) alginate	1935:1971	10% (w/v) ES100 and 1% (w/v) alginate	1935:1971	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	11	91	contain	containing	1924:1933	arg1	formulation					1912:1922	the formulation	1908:1922	the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate	1908:1971	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	11	92	theme	gelled	2022:2027	arg1	microparticles					2029:2042	the gelled microparticles	2018:2042	the gelled microparticles	2018:2042	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	6	93	theme	continuous	1193:1202	arg1	phases					1204:1209	the dispersed and continuous phases	1175:1209	the dispersed and continuous phases	1175:1209	A novelty of the work reported here is that the size of the generated monodispersed droplets could be precisely controlled in the range 50 μm-200 μm by varying the flow rates of the dispersed and continuous phases.					
29886256	6	94	theme	work	1014:1017	arg1	novelty					999:1005	A novelty	997:1005	A novelty of the work reported here	997:1031	A novelty of the work reported here is that the size of the generated monodispersed droplets could be precisely controlled in the range 50 μm-200 μm by varying the flow rates of the dispersed and continuous phases.					
29886256	8	95	theme	better	1462:1467	arg1	protection					1474:1483	better acid protection	1462:1483	better acid protection	1462:1483	Bigger microparticles (∼100 μm) showed better acid protection compared with smaller beads (∼50 μm) made from the same formulation.					
29886256	6	96	theme	dispersed	1179:1187	arg1	phases					1204:1209	the dispersed and continuous phases	1175:1209	the dispersed and continuous phases	1175:1209	A novelty of the work reported here is that the size of the generated monodispersed droplets could be precisely controlled in the range 50 μm-200 μm by varying the flow rates of the dispersed and continuous phases.					
29886256	4	97	theme	specialist	686:695	arg1	facilities					706:715	specialist research facilities	686:715	specialist research facilities	686:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	2	98	theme	Eudragit®	279:287	arg1	S100					289:292	a pH responsive methacrylic acid copolymer Eudragit® S100	236:292	a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v))	236:304	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	11	99	dep	%	1937:1937	arg1	w/v					1959:1961	w/v	1959:1961	w/v	1959:1961	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	11	99	dep	%	1937:1937	arg1	%					1956:1956	ES100 and 1%	1945:1956	ES100 and 1%	1945:1956	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	11	99	dep	%	1937:1937	arg1	w/v					1940:1942	w/v	1940:1942	w/v	1940:1942	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	4	100	theme	devices	649:655	arg1	chips					588:592	readily available low cost microfluidic chips	548:592	readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	548:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	5	101	theme	co-flow	737:743	arg1	devices					758:764	these co-flow microfluidic devices	731:764	these co-flow microfluidic devices	731:764	We show that these co-flow microfluidic devices can easily be used to prepare phage encapsulated microparticles making them suitable for use by both the phage research community and industry in order to evaluate and optimise phage compatible formulations for microencapsulation.					
29886256	5	102	theme	phage	871:875	arg1	community					886:894	the phage research community	867:894	the phage research community	867:894	We show that these co-flow microfluidic devices can easily be used to prepare phage encapsulated microparticles making them suitable for use by both the phage research community and industry in order to evaluate and optimise phage compatible formulations for microencapsulation.					
29886256	11	103	theme	encapsulation	2105:2117	arg1	matrix					2119:2124	the encapsulation matrix	2101:2124	the encapsulation matrix	2101:2124	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	13	104	theme	triggered	2486:2494	arg1	profile					2504:2510	a rapid pH triggered release profile	2475:2510	a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract	2475:2594	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	3	105	theme	generation	515:524	arg1	devices					526:532	commercially available microfluidic droplet generation devices	471:532	commercially available microfluidic droplet generation devices	471:532	The microencapsulation process employed commercially available microfluidic droplet generation devices.					
29886256	12	106	theme	Encapsulated	2127:2138	arg1	phages					2140:2145	Encapsulated phages	2127:2145	Encapsulated phages previously exposed to SGF (pH 1 for 2 h)	2127:2186	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	5	107	theme	compatible	949:958	arg1	formulations					960:971	phage compatible formulations	943:971	phage compatible formulations for microencapsulation	943:994	We show that these co-flow microfluidic devices can easily be used to prepare phage encapsulated microparticles making them suitable for use by both the phage research community and industry in order to evaluate and optimise phage compatible formulations for microencapsulation.					
29886256	0	108	theme	precision	5:13	arg1	microencapsulation					28:45	High precision microfluidic microencapsulation	0:45	High precision microfluidic microencapsulation of bacteriophages for enteric delivery.	0:85	High precision microfluidic microencapsulation of bacteriophages for enteric delivery.					
29886256	1	109	theme	specific	100:107	arg1	Myoviridae					133:142	Myoviridae	133:142	Myoviridae	133:142	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	1	109	theme	specific	100:107	arg1	O1					129:130	A Salmonella specific bacteriophage Felix O1	87:130	A Salmonella specific bacteriophage Felix O1 (Myoviridae)	87:143	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	10	110	from	ease	1771:1774	arg1	device					1818:1823	the microfluidic device	1801:1823	the microfluidic device	1801:1823	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	7	111	theme	release	1306:1312	arg1	profile					1314:1320	the phage release profile	1296:1320	the phage release profile	1296:1320	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	2	112	theme	%	412:412	arg1	composition					363:373	the composition	359:373	the composition	359:373	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	112	theme	%	412:412	arg1	w/v					424:426	w/v	424:426	w/v	424:426	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	112	theme	%	412:412	arg1	varied					388:393	varied	388:393	varied	388:393	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	112	theme	%	412:412	arg1	%					421:421	0.5% (w/v)-2%	409:421	0.5% (w/v)-2% (w/v)	409:427	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	12	113	theme	log	2302:2304	arg1	phase					2313:2317	the log growth phase	2298:2317	the log growth phase	2298:2317	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	13	114	theme	rapid	2477:2481	arg1	profile					2504:2510	a rapid pH triggered release profile	2475:2510	a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract	2475:2594	We have therefore demonstrated the microencapsulation of phages using readily available microfluidic chips to produce solid dosage microcapsule forms with a rapid pH triggered release profile suitable for targeted delivery and controlled release in the gastrointestinal tract.					
29886256	4	115	from	facilities	706:715	arg1	accessible					667:676	accessible	667:676	accessible	667:676	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	1	116	theme	Felix	123:127	arg1	Myoviridae					133:142	Myoviridae	133:142	Myoviridae	133:142	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	1	116	theme	Felix	123:127	arg1	O1					129:130	A Salmonella specific bacteriophage Felix O1	87:130	A Salmonella specific bacteriophage Felix O1 (Myoviridae)	87:143	A Salmonella specific bacteriophage Felix O1 (Myoviridae) was microencapsulated in a pH responsive polymer formulation.					
29886256	10	117	theme	alginate	1726:1733	arg1	amounts					1715:1721	higher amounts	1708:1721	higher amounts of alginate (e.g. 2% (w/v))	1708:1749	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	10	117	theme	alginate	1726:1733	arg1	alginate					1726:1733	alginate	1726:1733	alginate	1726:1733	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	0	118	theme	bacteriophages	50:63	arg1	microencapsulation					28:45	High precision microfluidic microencapsulation	0:45	High precision microfluidic microencapsulation of bacteriophages for enteric delivery.	0:85	High precision microfluidic microencapsulation of bacteriophages for enteric delivery.					
29886256	12	119	theme	bacterial	2278:2286	arg1	growth					2288:2293	bacterial growth	2278:2293	bacterial growth	2278:2293	Encapsulated phages previously exposed to SGF (pH 1 for 2 h) were released at elevated pH in simulated intestinal fluid (SIF) and were shown to arrest bacterial growth in the log growth phase.					
29886256	2	120	dep	varied	388:393	arg1	composition					363:373	the composition	359:373	the composition	359:373	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	120	dep	varied	388:393	arg1	w/v					424:426	w/v	424:426	w/v	424:426	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	120	dep	varied	388:393	arg1	varied					388:393	varied	388:393	varied	388:393	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	120	dep	varied	388:393	arg1	%					421:421	0.5% (w/v)-2%	409:421	0.5% (w/v)-2% (w/v)	409:427	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	0	121	theme	enteric	69:75	arg1	delivery					77:84	enteric delivery	69:84	enteric delivery	69:84	High precision microfluidic microencapsulation of bacteriophages for enteric delivery.					
29886256	2	122	theme	w/v	415:417	arg1	composition					363:373	the composition	359:373	the composition	359:373	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	122	theme	w/v	415:417	arg1	w/v					424:426	w/v	424:426	w/v	424:426	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	122	theme	w/v	415:417	arg1	varied					388:393	varied	388:393	varied	388:393	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	2	122	theme	w/v	415:417	arg1	%					421:421	0.5% (w/v)-2%	409:421	0.5% (w/v)-2% (w/v)	409:427	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	11	123	theme	10	1935:1936	arg1	%					1937:1937	%	1937:1937	%	1937:1937	Felix O1 encapsulated in the formulation containing 10% (w/v) ES100 and 1% (w/v) alginate showed excellent protection upon exposure of the gelled microparticles to SGF (pH 1 for 2 h) without the use of any antacids in the encapsulation matrix.					
29886256	7	124	theme	microparticle	1253:1265	arg1	size					1267:1270	microparticle size	1253:1270	microparticle size	1253:1270	Consequently, alginate concentration and microparticle size were shown to influence the phage release profile and the degree of acid protection afforded to phages upon exposure to simulated gastric fluid (SGF).					
29886256	4	125	theme	low	566:568	arg1	chips					588:592	readily available low cost microfluidic chips	548:592	readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	548:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	10	126	theme	high	1669:1672	arg1	formulations					1684:1695	The high viscosity formulations	1665:1695	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v))	1665:1749	The high viscosity formulations containing higher amounts of alginate (e.g. 2% (w/v)) negatively affected ease of droplet generation in the microfluidic device thereby posing a limitation in terms of process scale-up.					
29886256	9	127	theme	acid	1611:1614	arg1	protection					1616:1625	improved acid protection	1602:1625	improved acid protection of phages for similar particle sizes	1602:1662	Increasing the alginate composition resulted in improved acid protection of phages for similar particle sizes.					
29886256	2	128	from	varied	388:393	arg1	range					402:406	the range	398:406	the range	398:406	The formulation incorporated a pH responsive methacrylic acid copolymer Eudragit® S100 (10% (w/v)) with the addition of the biopolymer sodium alginate, the composition of which was varied in the range (0.5% (w/v)-2% (w/v)).					
29886256	4	129	theme	microfluidic	575:586	arg1	chips					588:592	readily available low cost microfluidic chips	548:592	readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities	548:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
29886256	4	130	from	accessible	667:676	arg1	facilities					706:715	specialist research facilities	686:715	specialist research facilities	686:715	We have used readily available low cost microfluidic chips instead of bespoke in-house fabricated glass capillary devices which are accessible only in specialist research facilities.					
31061448	2	0	dep	control	452:458	arg1	R					465:465	R	465:465	R	465:465	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	0	dep	control	452:458	arg1	RB					461:462	RB	461:462	RB	461:462	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	1	1	theme	LED	238:240	arg1	light					242:246	red and blue LED light	225:246	red and blue LED light based on the same daily light integral (7.49 μmol·m-2)	225:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	2	2	dep	RB	461:462	arg1	B = 1:1					467:473	B = 1:1	467:473	B = 1:1	467:473	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	3	theme	B	495:495	arg1	R					379:379	R	379:379	R	379:379	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	3	theme	B	495:495	arg1	modes					483:487	mixed modes	477:487	mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B)	477:543	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	3	theme	B	495:495	arg1	light					439:443	simultaneous red and blue light	413:443	simultaneous red and blue light as the control (RB, R:B = 1:1)	413:474	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	3	theme	B	495:495	arg1	light					575:579	alternating red and blue light	550:579	alternating red and blue light	550:579	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	3	theme	B	495:495	arg1	light					372:376	monochromatic red light	354:376	monochromatic red light (R)	354:380	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	3	theme	B	495:495	arg1	light					402:406	monochromatic blue light	383:406	monochromatic blue light (B)	383:410	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	3	theme	B	495:495	arg1	B					409:409	B	409:409	B	409:409	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	4	with	light	575:579	arg1	intervals					598:606	alternating intervals	586:606	alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h)	586:668	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	5	with	modes	483:487	arg1	intervals					598:606	alternating intervals	586:606	alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h)	586:668	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	6	dep	R	492:492	arg1	RB					526:527	4 h R to 4 h RB	513:527	4 h R to 4 h RB	513:527	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	6	dep	R	492:492	arg1	R/RB/B					505:510	R/RB/B	505:510	R/RB/B	505:510	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	6	dep	R	492:492	arg1	B					542:542	then 4 h B	533:542	then 4 h B	533:542	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	7	with	light	372:376	arg1	intervals					598:606	alternating intervals	586:606	alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h)	586:668	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	8	theme	R	492:492	arg1	R					379:379	R	379:379	R	379:379	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	8	theme	R	492:492	arg1	modes					483:487	mixed modes	477:487	mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B)	477:543	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	8	theme	R	492:492	arg1	light					439:443	simultaneous red and blue light	413:443	simultaneous red and blue light as the control (RB, R:B = 1:1)	413:474	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	8	theme	R	492:492	arg1	light					575:579	alternating red and blue light	550:579	alternating red and blue light	550:579	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	8	theme	R	492:492	arg1	light					372:376	monochromatic red light	354:376	monochromatic red light (R)	354:380	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	8	theme	R	492:492	arg1	light					402:406	monochromatic blue light	383:406	monochromatic blue light (B)	383:410	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	8	theme	R	492:492	arg1	B					409:409	B	409:409	B	409:409	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	9	theme	alternating	550:560	arg1	light					575:579	alternating red and blue light	550:579	alternating red and blue light	550:579	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	10	theme	alternating	586:596	arg1	intervals					598:606	alternating intervals	586:606	alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h)	586:668	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	0	11	theme	light	97:101	arg1	modes					71:75	different lighting modes	52:75	different lighting modes of red and blue LED light	52:101	Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light.					
31061448	1	12	theme	lettuce	178:184	arg1	response					143:150	the growth response	132:150	the growth response	132:150	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	1	12	theme	lettuce	178:184	arg1	accumulation					162:173	sugar accumulation	156:173	sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2)	156:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	5	13	theme	sucrose	1303:1309	arg1	activity					1291:1298	the activity	1287:1298	the activity of sucrose synthesizing enzyme (SPS)	1287:1335	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	9	14	theme	optimal	1802:1808	arg1	R/B					1785:1787	R/B	1785:1787	R/B(1 h)	1785:1792	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	9	14	theme	optimal	1802:1808	arg1	strategy					1819:1826	the optimal lighting strategy	1798:1826	the optimal lighting strategy	1798:1826	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	9	15	from	taste	1855:1859	arg1	study					1902:1906	the present study	1890:1906	the present study	1890:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	7	16	located	detected	1447:1454	arg1	plants					1459:1464	plants	1459:1464	plants treated with R/B(1 h)	1459:1486	The highest contents of sucrose and starch accompanied with the strongest activity of SPS were detected in plants treated with R/B(1 h).					
31061448	7	16	located	detected	1447:1454	arg2	contents					1364:1371	The highest contents	1352:1371	The highest contents of sucrose and starch accompanied with the strongest activity of SPS	1352:1440	The highest contents of sucrose and starch accompanied with the strongest activity of SPS were detected in plants treated with R/B(1 h).					
31061448	9	17	from	yield	1848:1852	arg1	study					1902:1906	the present study	1890:1906	the present study	1890:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	1	18	dep	LED	238:240	arg1	blue					233:236	blue	233:236	blue	233:236	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	2	19	with	light	402:406	arg1	intervals					598:606	alternating intervals	586:606	alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h)	586:668	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	1	20	theme	present	108:114	arg1	study					116:120	The present study	104:120	The present study	104:120	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	2	21	dep	4 h	522:524	arg1	to					519:520	to	519:520	to	519:520	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	22	theme	red	426:428	arg1	light					439:443	simultaneous red and blue light	413:443	simultaneous red and blue light as the control (RB, R:B = 1:1)	413:474	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	5	23	dep	treated	951:957	arg1	plants					944:949	plants	944:949	plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment	944:1110	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	23	dep	treated	951:957	arg1	enhanced					1088:1095	enhanced	1088:1095	were significantly enhanced by R treatment	1069:1110	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	9	24	theme	lettuce	1840:1846	arg1	yield					1848:1852	lettuce yield	1840:1852	lettuce yield	1840:1852	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	0	25	theme	different	52:60	arg1	modes					71:75	different lighting modes	52:75	different lighting modes of red and blue LED light	52:101	Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light.					
31061448	5	26	theme	enzymes	1199:1205	arg1	activities					1167:1176	the activities	1163:1176	the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI)	1163:1260	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	1	27	theme	growth	136:141	arg1	response					143:150	the growth response	132:150	the growth response	132:150	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	7	28	theme	starch	1388:1393	arg1	contents					1364:1371	The highest contents	1352:1371	The highest contents of sucrose and starch accompanied with the strongest activity of SPS	1352:1440	The highest contents of sucrose and starch accompanied with the strongest activity of SPS were detected in plants treated with R/B(1 h).					
31061448	0	29	theme	red	80:82	arg1	light					97:101	red and blue LED light	80:101	red and blue LED light	80:101	Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light.					
31061448	7	30	theme	sucrose	1376:1382	arg1	contents					1364:1371	The highest contents	1352:1371	The highest contents of sucrose and starch accompanied with the strongest activity of SPS	1352:1440	The highest contents of sucrose and starch accompanied with the strongest activity of SPS were detected in plants treated with R/B(1 h).					
31061448	2	31	theme	blue	434:437	arg1	light					439:443	simultaneous red and blue light	413:443	simultaneous red and blue light as the control (RB, R:B = 1:1)	413:474	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	7	32	theme	strongest	1416:1424	arg1	activity					1426:1433	the strongest activity	1412:1433	the strongest activity of SPS	1412:1440	The highest contents of sucrose and starch accompanied with the strongest activity of SPS were detected in plants treated with R/B(1 h).					
31061448	9	33	theme	use	1872:1874	arg1	efficiency					1876:1885	energy use efficiency	1865:1885	energy use efficiency	1865:1885	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	0	34	theme	blue	88:91	arg1	light					97:101	red and blue LED light	80:101	red and blue LED light	80:101	Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light.					
31061448	2	35	theme	monochromatic	383:395	arg1	light					402:406	monochromatic blue light	383:406	monochromatic blue light (B)	383:410	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	35	theme	monochromatic	383:395	arg1	B					409:409	B	409:409	B	409:409	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	5	36	theme	sucrose	1181:1187	arg1	invertase					1220:1228	acid invertase	1215:1228	acid invertase (AI)	1215:1233	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	36	theme	sucrose	1181:1187	arg1	invertase					1247:1255	neutral invertase	1239:1255	neutral invertase (NI)	1239:1260	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	36	theme	sucrose	1181:1187	arg1	enzymes					1199:1205	sucrose degrading enzymes	1181:1205	sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI)	1181:1260	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	0	37	theme	Sugar	0:4	arg1	accumulation					6:17	Sugar accumulation	0:17	Sugar accumulation	0:17	Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light.					
31061448	1	38	theme	light	242:246	arg1	modes					216:220	different lighting modes	197:220	different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2)	197:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	3	39	theme	irradiation	705:715	arg1	modes					717:721	different irradiation modes	695:721	different irradiation modes	695:721	The Results showed that different irradiation modes led to obvious morphological changes in lettuce.					
31061448	4	40	theme	fresh	810:814	arg1	weight					824:829	fresh and dry weight	810:829	weight	824:829	Among all the treatments, the highest fresh and dry weight of lettuce shoot were both detected with R/B(1 h), significantly higher than the other treatments.					
31061448	9	41	theme	present	1894:1900	arg1	study					1902:1906	the present study	1890:1906	the present study	1890:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	8	42	theme	different	1647:1655	arg1	environment					1663:1673	different light environment	1647:1673	different light environment	1647:1673	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	5	43	theme	sweetness	1036:1044	arg1	TSI					1053:1055	TSI	1053:1055	TSI	1053:1055	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	43	theme	sweetness	1036:1044	arg1	index					1046:1050	the total sweetness index	1026:1050	the total sweetness index (TSI) of lettuce	1026:1067	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	2	44	theme	red	368:370	arg1	R					379:379	R	379:379	R	379:379	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	44	theme	red	368:370	arg1	light					372:376	monochromatic red light	354:376	monochromatic red light (R)	354:380	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	1	45	theme	same	261:264	arg1	light					272:276	the same daily light integral	257:285	the same daily light integral (7.49 μmol·m-2)	257:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	1	45	theme	same	261:264	arg1	7.49 μmol·m-2					288:300	7.49 μmol·m-2	288:300	7.49 μmol·m-2	288:300	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	3	46	theme	obvious	730:736	arg1	changes					752:758	obvious morphological changes	730:758	obvious morphological changes in lettuce	730:769	The Results showed that different irradiation modes led to obvious morphological changes in lettuce.					
31061448	8	47	theme	sucrose	1743:1749	arg1	enzymes					1762:1768	sucrose metabolism enzymes	1743:1768	sucrose metabolism enzymes	1743:1768	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	8	48	theme	enzymes	1762:1768	arg1	activities					1729:1738	the activities	1725:1738	the activities of sucrose metabolism enzymes	1725:1768	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	4	49	theme	shoot	842:846	arg1	weight					824:829	fresh and dry weight	810:829	weight	824:829	Among all the treatments, the highest fresh and dry weight of lettuce shoot were both detected with R/B(1 h), significantly higher than the other treatments.					
31061448	1	50	theme	different	197:205	arg1	modes					216:220	different lighting modes	197:220	different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2)	197:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	2	51	theme	4 h	611:613	arg1	intervals					598:606	alternating intervals	586:606	alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h)	586:668	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	8	52	dep	treatments	1505:1514	arg1	1 h					1533:1535	1 h	1533:1535	1 h	1533:1535	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	8	52	dep	treatments	1505:1514	arg1	4 h					1520:1522	4 h	1520:1522	4 h	1520:1522	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	8	52	dep	treatments	1505:1514	arg1	R/B					1529:1531	R/B	1529:1531	R/B(1 h)	1529:1536	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	8	52	dep	treatments	1505:1514	arg1	R/B					1516:1518	R/B	1516:1518	R/B(4 h)	1516:1523	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	8	52	dep	treatments	1505:1514	arg1	treatments					1505:1514	The alternating treatments	1489:1514	The alternating treatments R/B(4 h) and R/B(1 h)	1489:1536	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	5	53	theme	lettuce	1061:1067	arg1	TSI					1053:1055	TSI	1053:1055	TSI	1053:1055	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	53	theme	lettuce	1061:1067	arg1	index					1046:1050	the total sweetness index	1026:1050	the total sweetness index (TSI) of lettuce	1026:1067	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	4	54	theme	dry	820:822	arg1	weight					824:829	fresh and dry weight	810:829	weight	824:829	Among all the treatments, the highest fresh and dry weight of lettuce shoot were both detected with R/B(1 h), significantly higher than the other treatments.					
31061448	9	55	from	strategy	1819:1826	arg1	terms					1831:1835	terms	1831:1835	terms of lettuce yield, taste and energy use efficiency in the present study	1831:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	9	55	from	strategy	1819:1826	arg1	whole					1778:1782	whole	1778:1782	whole	1778:1782	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	2	56	theme	lighting	308:315	arg1	treatments					317:326	Six lighting treatments	304:326	Six lighting treatments	304:326	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	1	57	theme	red	225:227	arg1	light					242:246	red and blue LED light	225:246	red and blue LED light based on the same daily light integral (7.49 μmol·m-2)	225:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	2	58	theme	mixed	477:481	arg1	modes					483:487	mixed modes	477:487	mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B)	477:543	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	59	theme	blue	570:573	arg1	light					575:579	alternating red and blue light	550:579	alternating red and blue light	550:579	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	60	theme	red	562:564	arg1	light					575:579	alternating red and blue light	550:579	alternating red and blue light	550:579	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	1	61	theme	sugar	156:160	arg1	accumulation					162:173	sugar accumulation	156:173	sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2)	156:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	5	62	theme	monochromatic	1124:1136	arg1	meanwhile					1113:1121	meanwhile	1113:1121	meanwhile	1113:1121	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	62	theme	monochromatic	1124:1136	arg1	R					1138:1138	monochromatic R	1124:1138	monochromatic R	1124:1138	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	8	63	theme	SS	1564:1565	arg1	activity					1552:1559	the activity	1548:1559	the activity of SS	1548:1565	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	2	64	theme	1 h	619:621	arg1	intervals					598:606	alternating intervals	586:606	alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h)	586:668	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	9	65	theme	lighting	1810:1817	arg1	R/B					1785:1787	R/B	1785:1787	R/B(1 h)	1785:1792	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	9	65	theme	lighting	1810:1817	arg1	strategy					1819:1826	the optimal lighting strategy	1798:1826	the optimal lighting strategy	1798:1826	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	5	66	theme	crude	1003:1007	arg1	fiber					1009:1013	crude fiber	1003:1013	crude fiber	1003:1013	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	66	theme	crude	1003:1007	arg1	RB					964:965	RB	964:965	RB	964:965	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	9	67	from	terms	1831:1835	arg1	study					1902:1906	the present study	1890:1906	the present study	1890:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	9	68	from	study	1902:1906	arg1	terms					1831:1835	terms	1831:1835	terms of lettuce yield, taste and energy use efficiency in the present study	1831:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	7	69	theme	SPS	1438:1440	arg1	activity					1426:1433	the strongest activity	1412:1433	the strongest activity of SPS	1412:1440	The highest contents of sucrose and starch accompanied with the strongest activity of SPS were detected in plants treated with R/B(1 h).					
31061448	0	70	theme	lighting	62:69	arg1	modes					71:75	different lighting modes	52:75	different lighting modes of red and blue LED light	52:101	Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light.					
31061448	2	71	theme	simultaneous	413:424	arg1	light					439:443	simultaneous red and blue light	413:443	simultaneous red and blue light as the control (RB, R:B = 1:1)	413:474	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	4	72	theme	other	912:916	arg1	treatments					918:927	the other treatments	908:927	the other treatments	908:927	Among all the treatments, the highest fresh and dry weight of lettuce shoot were both detected with R/B(1 h), significantly higher than the other treatments.					
31061448	5	73	theme	fructose	984:991	arg1	RB					964:965	RB	964:965	RB	964:965	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	73	theme	fructose	984:991	arg1	contents					972:979	the contents	968:979	the contents of fructose	968:991	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	9	74	theme	yield	1848:1852	arg1	terms					1831:1835	terms	1831:1835	terms of lettuce yield, taste and energy use efficiency in the present study	1831:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	9	75	from	efficiency	1876:1885	arg1	study					1902:1906	the present study	1890:1906	the present study	1890:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	9	76	theme	taste	1855:1859	arg1	terms					1831:1835	terms	1831:1835	terms of lettuce yield, taste and energy use efficiency in the present study	1831:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	8	77	theme	other	1613:1617	arg1	treatments					1619:1628	the other treatments	1609:1628	the other treatments	1609:1628	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	5	78	theme	acid	1215:1218	arg1	AI					1231:1232	AI	1231:1232	AI	1231:1232	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	78	theme	acid	1215:1218	arg1	invertase					1220:1228	acid invertase	1215:1228	acid invertase (AI)	1215:1233	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	2	79	theme	RB	501:502	arg1	R					379:379	R	379:379	R	379:379	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	79	theme	RB	501:502	arg1	modes					483:487	mixed modes	477:487	mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B)	477:543	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	79	theme	RB	501:502	arg1	light					439:443	simultaneous red and blue light	413:443	simultaneous red and blue light as the control (RB, R:B = 1:1)	413:474	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	79	theme	RB	501:502	arg1	light					575:579	alternating red and blue light	550:579	alternating red and blue light	550:579	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	79	theme	RB	501:502	arg1	light					372:376	monochromatic red light	354:376	monochromatic red light (R)	354:380	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	79	theme	RB	501:502	arg1	light					402:406	monochromatic blue light	383:406	monochromatic blue light (B)	383:410	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	79	theme	RB	501:502	arg1	B					409:409	B	409:409	B	409:409	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	9	80	theme	energy	1865:1870	arg1	efficiency					1876:1885	energy use efficiency	1865:1885	energy use efficiency	1865:1885	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	0	81	theme	LED	93:95	arg1	light					97:101	red and blue LED light	80:101	red and blue LED light	80:101	Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light.					
31061448	3	82	from	changes	752:758	arg1	lettuce					763:769	lettuce	763:769	lettuce	763:769	The Results showed that different irradiation modes led to obvious morphological changes in lettuce.					
31061448	9	83	theme	efficiency	1876:1885	arg1	terms					1831:1835	terms	1831:1835	terms of lettuce yield, taste and energy use efficiency in the present study	1831:1906	On the whole, R/B(1 h) was the optimal lighting strategy in terms of lettuce yield, taste and energy use efficiency in the present study.					
31061448	8	84	theme	alternating	1493:1503	arg1	treatments					1505:1514	The alternating treatments	1489:1514	The alternating treatments R/B(4 h) and R/B(1 h)	1489:1536	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	8	84	theme	alternating	1493:1503	arg1	R/B					1529:1531	R/B	1529:1531	R/B(1 h)	1529:1536	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	8	84	theme	alternating	1493:1503	arg1	R/B					1516:1518	R/B	1516:1518	R/B(4 h)	1516:1523	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	8	85	dep	enhanced	1574:1581	arg1	compared					1595:1602	compared	1595:1602	enhanced that of SPS compared with the other treatments	1574:1628	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	5	86	theme	degrading	1189:1197	arg1	invertase					1220:1228	acid invertase	1215:1228	acid invertase (AI)	1215:1233	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	86	theme	degrading	1189:1197	arg1	invertase					1247:1255	neutral invertase	1239:1255	neutral invertase (NI)	1239:1260	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	86	theme	degrading	1189:1197	arg1	enzymes					1199:1205	sucrose degrading enzymes	1181:1205	sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI)	1181:1260	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	8	87	theme	light	1657:1661	arg1	environment					1663:1673	different light environment	1647:1673	different light environment	1647:1673	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	2	88	with	light	439:443	arg1	intervals					598:606	alternating intervals	586:606	alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h)	586:668	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	7	89	theme	highest	1356:1362	arg1	contents					1364:1371	The highest contents	1352:1371	The highest contents of sucrose and starch accompanied with the strongest activity of SPS	1352:1440	The highest contents of sucrose and starch accompanied with the strongest activity of SPS were detected in plants treated with R/B(1 h).					
31061448	4	90	theme	highest	802:808	arg1	weight					824:829	fresh and dry weight	810:829	weight	824:829	Among all the treatments, the highest fresh and dry weight of lettuce shoot were both detected with R/B(1 h), significantly higher than the other treatments.					
31061448	5	91	theme	neutral	1239:1245	arg1	NI					1258:1259	NI	1258:1259	NI	1258:1259	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	91	theme	neutral	1239:1245	arg1	invertase					1247:1255	neutral invertase	1239:1255	neutral invertase (NI)	1239:1260	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	92	theme	total	1030:1034	arg1	TSI					1053:1055	TSI	1053:1055	TSI	1053:1055	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	5	92	theme	total	1030:1034	arg1	index					1046:1050	the total sweetness index	1026:1050	the total sweetness index (TSI) of lettuce	1026:1067	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	0	93	theme	lettuce	33:39	arg1	growth					23:28	growth	23:28	growth	23:28	Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light.					
31061448	0	93	theme	lettuce	33:39	arg1	accumulation					6:17	Sugar accumulation	0:17	Sugar accumulation	0:17	Sugar accumulation and growth of lettuce exposed to different lighting modes of red and blue LED light.					
31061448	3	94	theme	different	695:703	arg1	modes					717:721	different irradiation modes	695:721	different irradiation modes	695:721	The Results showed that different irradiation modes led to obvious morphological changes in lettuce.					
31061448	8	95	theme	metabolism	1751:1760	arg1	enzymes					1762:1768	sucrose metabolism enzymes	1743:1768	sucrose metabolism enzymes	1743:1768	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	2	96	theme	monochromatic	354:366	arg1	R					379:379	R	379:379	R	379:379	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	96	theme	monochromatic	354:366	arg1	light					372:376	monochromatic red light	354:376	monochromatic red light (R)	354:380	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	8	97	theme	sugar	1691:1695	arg1	compositions					1697:1708	sugar compositions	1691:1708	sugar compositions	1691:1708	The alternating treatments R/B(4 h) and R/B(1 h) inhibited the activity of SS, while enhanced that of SPS compared with the other treatments, indicating that different light environment might influence sugar compositions via regulating the activities of sucrose metabolism enzymes.					
31061448	1	98	theme	daily	266:270	arg1	light					272:276	the same daily light integral	257:285	the same daily light integral (7.49 μmol·m-2)	257:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	1	98	theme	daily	266:270	arg1	7.49 μmol·m-2					288:300	7.49 μmol·m-2	288:300	7.49 μmol·m-2	288:300	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	3	99	theme	morphological	738:750	arg1	changes					752:758	obvious morphological changes	730:758	obvious morphological changes in lettuce	730:769	The Results showed that different irradiation modes led to obvious morphological changes in lettuce.					
31061448	2	100	theme	blue	397:400	arg1	light					402:406	monochromatic blue light	383:406	monochromatic blue light (B)	383:410	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	2	100	theme	blue	397:400	arg1	B					409:409	B	409:409	B	409:409	Six lighting treatments were performed, that were monochromatic red light (R), monochromatic blue light (B), simultaneous red and blue light as the control (RB, R:B = 1:1), mixed modes of R, B and RB (R/RB/B, 4 h R to 4 h RB and then 4 h B), and alternating red and blue light with alternating intervals of 4 h and 1 h respectively recorded as R/B(4 h) and R/B(1 h).					
31061448	5	101	theme	R	1100:1100	arg1	treatment					1102:1110	R treatment	1100:1110	R treatment	1100:1110	Compared with plants treated with RB, the contents of fructose, glucose, crude fiber as well as the total sweetness index (TSI) of lettuce were significantly enhanced by R treatment; meanwhile, monochromatic R significantly promoted the activities of sucrose degrading enzymes such as acid invertase (AI) and neutral invertase (NI), while obviously reduced the activity of sucrose synthesizing enzyme (SPS).					
31061448	1	102	theme	integral	278:285	arg1	light					272:276	the same daily light integral	257:285	the same daily light integral (7.49 μmol·m-2)	257:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	1	102	theme	integral	278:285	arg1	7.49 μmol·m-2					288:300	7.49 μmol·m-2	288:300	7.49 μmol·m-2	288:300	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31061448	4	103	theme	lettuce	834:840	arg1	shoot					842:846	lettuce shoot	834:846	lettuce shoot	834:846	Among all the treatments, the highest fresh and dry weight of lettuce shoot were both detected with R/B(1 h), significantly higher than the other treatments.					
31061448	1	104	theme	lighting	207:214	arg1	modes					216:220	different lighting modes	197:220	different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2)	197:301	The present study evaluated the growth response and sugar accumulation of lettuce exposed to different lighting modes of red and blue LED light based on the same daily light integral (7.49 μmol·m-2).					
31499965	2	0	dep	co-incubation	803:815	arg1	the					799:801	the	799:801	the	799:801	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	1	1	theme	pure	495:498	arg1	tetrahydrate					516:527	the analytically pure calcium nitrate tetrahydrate	478:527	the analytically pure calcium nitrate tetrahydrate	478:527	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	10	2	theme	process	1750:1756	arg1	interpretation					1728:1741	An interpretation	1725:1741	An interpretation of the process of Zn ions' embedding into hydroxyapatite and alginate matrix	1725:1818	An interpretation of the process of Zn ions' embedding into hydroxyapatite and alginate matrix is given, as well as their influence on the biomimetic composite properties is discussed in details.					
31499965	1	3	theme	properties	288:297	arg1	studies					242:248	studies	242:248	studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride	242:616	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	7	4	theme	TEM	1462:1464	arg1	analysis					1466:1473	TEM analysis	1462:1473	TEM analysis	1462:1473	The replacement was evidenced by the non-stoichiometric Ca/P ratio < 2.16, OPO lines' shifting on FTIR spectrum and TEM analysis.					
31499965	0	5	from	embedded	179:186	arg1	matrix					200:205	Alginate matrix	191:205	Alginate matrix	191:205	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	14	6	dep	Nowadays	2379:2386	arg1	we					2388:2389	we	2388:2389	we	2388:2389	Nowadays we have lack of information and the study's results about those interactions.					
31499965	1	7	theme	nitrate	508:514	arg1	tetrahydrate					516:527	the analytically pure calcium nitrate tetrahydrate	478:527	the analytically pure calcium nitrate tetrahydrate	478:527	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	1	8	theme	multiphase	302:311	arg1	materials					327:335	multiphase nanocomposite materials	302:335	multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride	302:616	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	14	9	theme	results	2432:2438	arg1	lack					2396:2399	lack	2396:2399	lack of information and the study's results about those interactions	2396:2463	Nowadays we have lack of information and the study's results about those interactions.					
31499965	6	10	theme	partial	1218:1224	arg1	replacement					1226:1236	a partial replacement	1216:1236	a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice	1216:1280	At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation.					
31499965	13	11	theme	phase	2234:2238	arg1	states					2240:2245	the phase states	2230:2245	the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate	2230:2351	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	15	12	theme	composite	2798:2806	arg1	components					2808:2817	another composite components	2790:2817	another composite components	2790:2817	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	13	theme	materials	2629:2637	arg1	cytotoxicity					2573:2584	cytotoxicity	2573:2584	cytotoxicity	2573:2584	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	13	theme	materials	2629:2637	arg1	biocompatibility					2552:2567	biocompatibility	2552:2567	biocompatibility	2552:2567	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	12	14	theme	zinc-doped	2142:2151	arg1	hydroxyapatite					2153:2166	zinc-doped hydroxyapatite	2142:2166	zinc-doped hydroxyapatite	2142:2166	Zinc plays an important role in the creation of new antimicrobial agents, and zinc-doped hydroxyapatite will find further application in biomedicine.					
31499965	11	15	theme	fungi	2057:2061	arg1	development					2023:2033	development	2023:2033	development	2023:2033	STATEMENT OF SIGNIFICANCE: A number of studies have shown that Zn effectively inhibits the growth and development of bacteria and yeast fungi.					
31499965	11	15	theme	fungi	2057:2061	arg1	growth					2012:2017	growth	2012:2017	growth	2012:2017	STATEMENT OF SIGNIFICANCE: A number of studies have shown that Zn effectively inhibits the growth and development of bacteria and yeast fungi.					
31499965	15	16	theme	nanostructured	2604:2617	arg1	such					2640:2643	such	2640:2643	such	2640:2643	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	16	theme	nanostructured	2604:2617	arg1	materials					2629:2637	the biomimetic nanostructured composite materials	2589:2637	the biomimetic nanostructured composite materials	2589:2637	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	16	theme	nanostructured	2604:2617	arg1	hydrogel					2727:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	4	17	theme	synthesized	972:982	arg1	material					998:1005	synthesized nanocomposite material	972:1005	synthesized nanocomposite material	972:1005	It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal.					
31499965	6	18	theme	same	1205:1208	arg1	time					1210:1213	the same time	1201:1213	the same time	1201:1213	At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation.					
31499965	2	19	theme	agar	831:834	arg1	methods					847:853	modified "agar diffusion" methods	821:853	modified "agar diffusion" methods	821:853	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	0	20	theme	hydroxyapatite-biphasic	126:148	arg1	micro-					154:159	hydroxyapatite-biphasic ZnO micro-	126:159	hydroxyapatite-biphasic ZnO micro-	126:159	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	5	21	theme	possible	1129:1136	arg1	explanation					1138:1148	The possible explanation	1125:1148	The possible explanation of ZnO nanocrystals' phase transition	1125:1186	The possible explanation of ZnO nanocrystals' phase transition is given.					
31499965	13	22	theme	calcium	2290:2296	arg1	replacement					2298:2308	calcium replacement	2290:2308	calcium replacement by zinc	2290:2316	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	7	23	theme	OPO	1421:1423	arg1	ratio < 2.16					1407:1418	the non-stoichiometric Ca/P ratio < 2.16	1379:1418	the non-stoichiometric Ca/P ratio < 2.16	1379:1418	The replacement was evidenced by the non-stoichiometric Ca/P ratio < 2.16, OPO lines' shifting on FTIR spectrum and TEM analysis.					
31499965	7	23	theme	OPO	1421:1423	arg1	lines					1425:1429	OPO lines	1421:1429	OPO lines	1421:1429	The replacement was evidenced by the non-stoichiometric Ca/P ratio < 2.16, OPO lines' shifting on FTIR spectrum and TEM analysis.					
31499965	2	24	theme	modified	821:828	arg1	methods					847:853	modified "agar diffusion" methods	821:853	modified "agar diffusion" methods	821:853	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	12	25	theme	antimicrobial	2116:2128	arg1	agents					2130:2135	new antimicrobial agents	2112:2135	new antimicrobial agents	2112:2135	Zinc plays an important role in the creation of new antimicrobial agents, and zinc-doped hydroxyapatite will find further application in biomedicine.					
31499965	8	26	theme	studied	1480:1486	arg1	composite					1488:1496	The studied composite	1476:1496	The studied composite	1476:1496	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	3	27	used	used	889:892	arg2	cells					878:882	The murine fibroblast cells	856:882	The murine fibroblast cells	856:882	The murine fibroblast cells were used for the biocompatibility tests and cytotoxicity evaluation.					
31499965	2	28	theme	antimicrobial	632:644	arg1	assessment					655:664	The samples' antimicrobial activity assessment	619:664	The samples' antimicrobial activity assessment	619:664	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	6	29	theme	Zn2+	1254:1257	arg1	ions					1259:1262	Zn2+ ions	1254:1262	Zn2+ ions	1254:1262	At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation.					
31499965	7	30	theme	non-stoichiometric	1383:1400	arg1	ratio < 2.16					1407:1418	the non-stoichiometric Ca/P ratio < 2.16	1379:1418	the non-stoichiometric Ca/P ratio < 2.16	1379:1418	The replacement was evidenced by the non-stoichiometric Ca/P ratio < 2.16, OPO lines' shifting on FTIR spectrum and TEM analysis.					
31499965	7	30	theme	non-stoichiometric	1383:1400	arg1	lines					1425:1429	OPO lines	1421:1429	OPO lines	1421:1429	The replacement was evidenced by the non-stoichiometric Ca/P ratio < 2.16, OPO lines' shifting on FTIR spectrum and TEM analysis.					
31499965	13	31	theme	processes	2277:2285	arg1	states					2240:2245	the phase states	2230:2245	the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate	2230:2351	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	8	32	theme	pronounced	1512:1521	arg1	activity					1537:1544	a pronounced antibacterial activity	1510:1544	a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite	1510:1646	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	6	33	theme	ions	1246:1249	arg1	replacement					1226:1236	a partial replacement	1216:1236	a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice	1216:1280	At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation.					
31499965	2	34	theme	test	782:785	arg1	cultures					787:794	test cultures	782:794	test cultures	782:794	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	15	35	dep	morphology	2516:2525	arg1	patterns					2776:2783	patterns	2776:2783	patterns	2776:2783	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	0	36	theme	antibacterial	59:71	arg1	properties					73:82	antibacterial properties	59:82	antibacterial properties	59:82	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	1	37	theme	zinc	398:401	arg1	oxide					403:407	hydroxyapatite‑sodium alginate-biphasic zinc oxide	358:407	hydroxyapatite‑sodium alginate-biphasic zinc oxide	358:407	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	1	37	theme	zinc	398:401	arg1	HA-Alg-ZnO					346:355	HA-Alg-ZnO	346:355	HA-Alg-ZnO	346:355	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	13	38	theme	oxide	2255:2259	arg1	states					2240:2245	the phase states	2230:2245	the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate	2230:2351	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	3	39	theme	cytotoxicity	929:940	arg1	evaluation					942:951	cytotoxicity evaluation	929:951	cytotoxicity evaluation	929:951	The murine fibroblast cells were used for the biocompatibility tests and cytotoxicity evaluation.					
31499965	4	40	theme	ZnO	1054:1056	arg1	nanocrystals					1058:1069	ZnO nanocrystals	1054:1069	ZnO nanocrystals	1054:1069	It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal.					
31499965	15	41	theme	hydroxyapatite/ZnO	2702:2719	arg1	materials					2629:2637	the biomimetic nanostructured composite materials	2589:2637	the biomimetic nanostructured composite materials	2589:2637	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	41	theme	hydroxyapatite/ZnO	2702:2719	arg1	hydrogel					2727:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	42	theme	multiphase	2505:2514	arg1	morphology					2516:2525	the multiphase morphology	2501:2525	the multiphase morphology	2501:2525	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	3	43	theme	murine	860:865	arg1	cells					878:882	The murine fibroblast cells	856:882	The murine fibroblast cells	856:882	The murine fibroblast cells were used for the biocompatibility tests and cytotoxicity evaluation.					
31499965	0	44	theme	nanocomposite	93:105	arg1	materials					107:115	nanocomposite materials	93:115	nanocomposite materials	93:115	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	4	45	theme	nanoscale	1024:1032	arg1	architecture					1034:1045	a multiphase nanoscale architecture	1011:1045	a multiphase nanoscale architecture	1011:1045	It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal.					
31499965	13	46	from	states	2240:2245	arg1	alginate					2344:2351	alginate	2344:2351	alginate	2344:2351	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	13	46	from	states	2240:2245	arg1	apatite					2329:2335	calcium apatite	2321:2335	calcium apatite	2321:2335	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	15	47	theme	alginate/hydroxyapatite/ZnO	2655:2681	arg1	granules					2689:2696	sodium alginate/hydroxyapatite/ZnO based granules	2648:2696	sodium alginate/hydroxyapatite/ZnO based granules	2648:2696	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	1	48	theme	hydroxyapatite‑zinc	422:440	arg1	HA-ZnO					414:419	HA-ZnO	414:419	HA-ZnO	414:419	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	1	48	theme	hydroxyapatite‑zinc	422:440	arg1	oxide					442:446	hydroxyapatite‑zinc oxide	422:446	hydroxyapatite‑zinc oxide	422:446	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	8	49	theme	ZnO	1574:1576	arg1	particles					1578:1586	ZnO particles	1574:1586	ZnO particles	1574:1586	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	6	50	from	replacement	1226:1236	arg1	lattice					1274:1280	the HA lattice	1267:1280	the HA lattice	1267:1280	At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation.					
31499965	1	51	theme	nanocomposite	313:325	arg1	materials					327:335	multiphase nanocomposite materials	302:335	multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride	302:616	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	15	52	theme	present	2470:2476	arg1	study					2478:2482	The present study	2466:2482	The present study	2466:2482	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	4	53	theme	cubic	1104:1108	arg1	lattices					1094:1101	two lattices	1090:1101	two lattices: cubic and hexagonal	1090:1122	It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal.					
31499965	0	54	from	matrix	200:205	arg1	embedded					179:186	embedded	179:186	embedded	179:186	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	10	55	theme	composite	1875:1883	arg1	properties					1885:1894	the biomimetic composite properties	1860:1894	the biomimetic composite properties	1860:1894	An interpretation of the process of Zn ions' embedding into hydroxyapatite and alginate matrix is given, as well as their influence on the biomimetic composite properties is discussed in details.					
31499965	0	56	theme	in	29:30	arg1	biocompatibility					38:53	in vitro biocompatibility	29:53	in vitro biocompatibility	29:53	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	15	57	theme	establishing	2745:2756	arg1	ions					2761:2764	the establishing Zn ions	2741:2764	the establishing Zn ions' behavior	2741:2774	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	8	58	theme	moistened	1613:1621	arg1	powder					1623:1628	moistened powder	1613:1628	moistened powder	1613:1628	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	1	59	theme	hydroxyapatite‑sodium	358:378	arg1	oxide					403:407	hydroxyapatite‑sodium alginate-biphasic zinc oxide	358:407	hydroxyapatite‑sodium alginate-biphasic zinc oxide	358:407	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	1	59	theme	hydroxyapatite‑sodium	358:378	arg1	HA-Alg-ZnO					346:355	HA-Alg-ZnO	346:355	HA-Alg-ZnO	346:355	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	0	60	dep	in	29:30	arg1	vitro					32:36	vitro	32:36	vitro	32:36	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	4	61	contain	has	1007:1009	arg2	architecture					1034:1045	a multiphase nanoscale architecture	1011:1045	a multiphase nanoscale architecture	1011:1045	It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal.					
31499965	4	61	contain	has	1007:1009	arg1	material					998:1005	synthesized nanocomposite material	972:1005	synthesized nanocomposite material	972:1005	It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal.					
31499965	1	62	theme	zinc	572:575	arg1	hexahydrate					585:595	zinc nitrate hexahydrate	572:595	zinc nitrate hexahydrate	572:595	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	1	63	theme	studies	242:248	arg1	results					231:237	the results	227:237	the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride	227:616	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	12	64	theme	further	2178:2184	arg1	application					2186:2196	further application	2178:2196	further application in biomedicine	2178:2211	Zinc plays an important role in the creation of new antimicrobial agents, and zinc-doped hydroxyapatite will find further application in biomedicine.					
31499965	13	65	from	apatite	2329:2335	arg1	states					2240:2245	the phase states	2230:2245	the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate	2230:2351	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	10	66	theme	ions	1764:1767	arg1	process					1750:1756	the process	1746:1756	the process of Zn ions' embedding into hydroxyapatite and alginate matrix	1746:1818	An interpretation of the process of Zn ions' embedding into hydroxyapatite and alginate matrix is given, as well as their influence on the biomimetic composite properties is discussed in details.					
31499965	12	67	from	application	2186:2196	arg1	biomedicine					2201:2211	biomedicine	2201:2211	biomedicine	2201:2211	Zinc plays an important role in the creation of new antimicrobial agents, and zinc-doped hydroxyapatite will find further application in biomedicine.					
31499965	1	68	theme	biocompatibility	253:268	arg1	studies					242:248	studies	242:248	studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride	242:616	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	0	69	theme	Alginate	191:198	arg1	matrix					200:205	Alginate matrix	191:205	Alginate matrix	191:205	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	0	70	dep	Synthesis	0:8	arg1	study					84:88	study	84:88	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials	0:115	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	4	71	theme	hexagonal	1114:1122	arg1	lattices					1094:1101	two lattices	1090:1101	two lattices: cubic and hexagonal	1090:1122	It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal.					
31499965	6	72	theme	composite	1319:1327	arg1	processing					1305:1314	processing	1305:1314	processing of composite by US radiation	1305:1343	At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation.					
31499965	1	73	theme	calcium	601:607	arg1	chloride					609:616	calcium chloride	601:616	calcium chloride	601:616	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	1	74	theme	antibacterial	274:286	arg1	properties					288:297	antibacterial properties	274:297	antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride	274:616	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	9	75	theme	HA-Alg-ZnO	1687:1696	arg1	forms					1654:1658	Both forms	1649:1658	Both forms of HA-ZnO (suspension) and HA-Alg-ZnO (beads)	1649:1704	Both forms of HA-ZnO (suspension) and HA-Alg-ZnO (beads) are biocompatible.					
31499965	13	76	from	processes	2277:2285	arg1	alginate					2344:2351	alginate	2344:2351	alginate	2344:2351	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	13	76	from	processes	2277:2285	arg1	apatite					2329:2335	calcium apatite	2321:2335	calcium apatite	2321:2335	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	1	77	theme	calcium	500:506	arg1	tetrahydrate					516:527	the analytically pure calcium nitrate tetrahydrate	478:527	the analytically pure calcium nitrate tetrahydrate	478:527	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	15	78	theme	antimicrobial	2528:2540	arg1	activity					2542:2549	antimicrobial activity	2528:2549	antimicrobial activity	2528:2549	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	13	79	from	oxide	2255:2259	arg1	alginate					2344:2351	alginate	2344:2351	alginate	2344:2351	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	13	79	from	oxide	2255:2259	arg1	apatite					2329:2335	calcium apatite	2321:2335	calcium apatite	2321:2335	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	12	80	theme	agents	2130:2135	arg1	creation					2100:2107	the creation	2096:2107	the creation of new antimicrobial agents	2096:2135	Zinc plays an important role in the creation of new antimicrobial agents, and zinc-doped hydroxyapatite will find further application in biomedicine.					
31499965	1	81	theme	ammonium	530:537	arg1	hydrophosphate					539:552	ammonium hydrophosphate	530:552	ammonium hydrophosphate	530:552	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	11	82	theme	yeast	2051:2055	arg1	fungi					2057:2061	yeast fungi	2051:2061	yeast fungi	2051:2061	STATEMENT OF SIGNIFICANCE: A number of studies have shown that Zn effectively inhibits the growth and development of bacteria and yeast fungi.					
31499965	13	83	theme	calcium	2321:2327	arg1	apatite					2329:2335	calcium apatite	2321:2335	calcium apatite	2321:2335	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	2	84	dep	Gram-negative	685:697	arg1	aeruginosa					712:721	P. aeruginosa	709:721	P. aeruginosa	709:721	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	2	84	dep	Gram-negative	685:697	arg1	cultures					787:794	test cultures	782:794	test cultures	782:794	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	2	84	dep	Gram-negative	685:697	arg1	coli					703:706	E. coli	700:706	E. coli	700:706	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	7	85	theme	FTIR	1444:1447	arg1	spectrum					1449:1456	FTIR spectrum	1444:1456	FTIR spectrum	1444:1456	The replacement was evidenced by the non-stoichiometric Ca/P ratio < 2.16, OPO lines' shifting on FTIR spectrum and TEM analysis.					
31499965	2	86	theme	diffusion	836:844	arg1	methods					847:853	modified "agar diffusion" methods	821:853	modified "agar diffusion" methods	821:853	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	0	87	theme	ZnO	150:152	arg1	micro-					154:159	hydroxyapatite-biphasic ZnO micro-	126:159	hydroxyapatite-biphasic ZnO micro-	126:159	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	10	88	theme	alginate	1804:1811	arg1	matrix					1813:1818	alginate matrix	1804:1818	alginate matrix	1804:1818	An interpretation of the process of Zn ions' embedding into hydroxyapatite and alginate matrix is given, as well as their influence on the biomimetic composite properties is discussed in details.					
31499965	15	89	theme	composite	2619:2627	arg1	such					2640:2643	such	2640:2643	such	2640:2643	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	89	theme	composite	2619:2627	arg1	materials					2629:2637	the biomimetic nanostructured composite materials	2589:2637	the biomimetic nanostructured composite materials	2589:2637	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	89	theme	composite	2619:2627	arg1	hydrogel					2727:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	90	theme	biomimetic	2593:2602	arg1	such					2640:2643	such	2640:2643	such	2640:2643	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	90	theme	biomimetic	2593:2602	arg1	materials					2629:2637	the biomimetic nanostructured composite materials	2589:2637	the biomimetic nanostructured composite materials	2589:2637	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	90	theme	biomimetic	2593:2602	arg1	hydrogel					2727:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	13	91	theme	replacement	2298:2308	arg1	oxide					2255:2259	zinc oxide	2250:2259	zinc oxide	2250:2259	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	13	91	theme	replacement	2298:2308	arg1	processes					2277:2285	the processes	2273:2285	the processes of calcium replacement by zinc in calcium apatite and in alginate	2273:2351	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	4	92	theme	nanocomposite	984:996	arg1	material					998:1005	synthesized nanocomposite material	972:1005	synthesized nanocomposite material	972:1005	It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal.					
31499965	12	93	theme	new	2112:2114	arg1	agents					2130:2135	new antimicrobial agents	2112:2135	new antimicrobial agents	2112:2135	Zinc plays an important role in the creation of new antimicrobial agents, and zinc-doped hydroxyapatite will find further application in biomedicine.					
31499965	15	94	theme	based	2683:2687	arg1	granules					2689:2696	sodium alginate/hydroxyapatite/ZnO based granules	2648:2696	sodium alginate/hydroxyapatite/ZnO based granules	2648:2696	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	5	95	theme	ZnO	1153:1155	arg1	transition					1177:1186	ZnO nanocrystals' phase transition	1153:1186	ZnO nanocrystals' phase transition	1153:1186	The possible explanation of ZnO nanocrystals' phase transition is given.					
31499965	11	96	theme	studies	1960:1966	arg1	number					1950:1955	A number	1948:1955	A number of studies	1948:1966	STATEMENT OF SIGNIFICANCE: A number of studies have shown that Zn effectively inhibits the growth and development of bacteria and yeast fungi.					
31499965	2	97	theme	activity	646:653	arg1	assessment					655:664	The samples' antimicrobial activity assessment	619:664	The samples' antimicrobial activity assessment	619:664	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	12	98	theme	important	2078:2086	arg1	role					2088:2091	an important role	2075:2091	an important role	2075:2091	Zinc plays an important role in the creation of new antimicrobial agents, and zinc-doped hydroxyapatite will find further application in biomedicine.					
31499965	6	99	theme	US	1332:1333	arg1	radiation					1335:1343	US radiation	1332:1343	US radiation	1332:1343	At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation.					
31499965	3	100	theme	biocompatibility	902:917	arg1	tests					919:923	the biocompatibility tests	898:923	the biocompatibility tests	898:923	The murine fibroblast cells were used for the biocompatibility tests and cytotoxicity evaluation.					
31499965	8	101	theme	antibacterial	1523:1535	arg1	activity					1537:1544	a pronounced antibacterial activity	1510:1544	a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite	1510:1646	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	7	102	theme	Ca/P	1402:1405	arg1	ratio < 2.16					1407:1418	the non-stoichiometric Ca/P ratio < 2.16	1379:1418	the non-stoichiometric Ca/P ratio < 2.16	1379:1418	The replacement was evidenced by the non-stoichiometric Ca/P ratio < 2.16, OPO lines' shifting on FTIR spectrum and TEM analysis.					
31499965	7	102	theme	Ca/P	1402:1405	arg1	lines					1425:1429	OPO lines	1421:1429	OPO lines	1421:1429	The replacement was evidenced by the non-stoichiometric Ca/P ratio < 2.16, OPO lines' shifting on FTIR spectrum and TEM analysis.					
31499965	15	103	theme	based	2721:2725	arg1	materials					2629:2637	the biomimetic nanostructured composite materials	2589:2637	the biomimetic nanostructured composite materials	2589:2637	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	15	103	theme	based	2721:2725	arg1	hydrogel					2727:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	hydroxyapatite/ZnO based hydrogel	2702:2734	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	13	104	theme	zinc	2250:2253	arg1	oxide					2255:2259	zinc oxide	2250:2259	zinc oxide	2250:2259	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	2	105	dep	bacteria	742:749	arg1	aureus					755:760	S. aureus	752:760	S. aureus	752:760	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	2	105	dep	bacteria	742:749	arg1	epidermidis					769:779	S. epidermidis	766:779	S. epidermidis	766:779	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	8	106	theme	due	1546:1548	arg1	activity					1537:1544	a pronounced antibacterial activity	1510:1544	a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite	1510:1646	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	6	107	theme	Ca2+	1241:1244	arg1	ions					1246:1249	Ca2+ ions	1241:1249	Ca2+ ions	1241:1249	At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation.					
31499965	0	108	theme	materials	107:115	arg1	study					84:88	study	84:88	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials	0:115	Synthesis, characterization, in vitro biocompatibility and antibacterial properties study of nanocomposite materials based on hydroxyapatite-biphasic ZnO micro- and nanoparticles embedded in Alginate matrix.					
31499965	15	109	theme	morphology	2516:2525	arg1	data					2493:2496	data	2493:2496	data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns	2493:2783	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	13	110	from	alginate	2344:2351	arg1	states					2240:2245	the phase states	2230:2245	the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate	2230:2351	In this regard, the phase states of zinc oxide, as well as the processes of calcium replacement by zinc in calcium apatite and in alginate should be explored fully.					
31499965	3	111	theme	fibroblast	867:876	arg1	cells					878:882	The murine fibroblast cells	856:882	The murine fibroblast cells	856:882	The murine fibroblast cells were used for the biocompatibility tests and cytotoxicity evaluation.					
31499965	15	112	theme	sodium	2648:2653	arg1	granules					2689:2696	sodium alginate/hydroxyapatite/ZnO based granules	2648:2696	sodium alginate/hydroxyapatite/ZnO based granules	2648:2696	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	4	113	theme	multiphase	1013:1022	arg1	architecture					1034:1045	a multiphase nanoscale architecture	1011:1045	a multiphase nanoscale architecture	1011:1045	It was shown that synthesized nanocomposite material has a multiphase nanoscale architecture, where ZnO nanocrystals are represented by two lattices: cubic and hexagonal.					
31499965	8	114	theme	particles	1578:1586	arg1	incorporation					1557:1569	the incorporation	1553:1569	the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite	1553:1646	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	11	115	theme	SIGNIFICANCE	1934:1945	arg1	STATEMENT					1921:1929	STATEMENT	1921:1929	STATEMENT OF SIGNIFICANCE	1921:1945	STATEMENT OF SIGNIFICANCE: A number of studies have shown that Zn effectively inhibits the growth and development of bacteria and yeast fungi.					
31499965	8	116	theme	sodium	1593:1598	arg1	alginate					1600:1607	sodium alginate	1593:1607	sodium alginate	1593:1607	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	2	117	theme	Gram-positive	728:740	arg1	bacteria					742:749	Gram-positive bacteria	728:749	Gram-positive bacteria (S. aureus and S. epidermidis)	728:780	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	11	118	theme	bacteria	2038:2045	arg1	development					2023:2033	development	2023:2033	development	2023:2033	STATEMENT OF SIGNIFICANCE: A number of studies have shown that Zn effectively inhibits the growth and development of bacteria and yeast fungi.					
31499965	11	118	theme	bacteria	2038:2045	arg1	growth					2012:2017	growth	2012:2017	growth	2012:2017	STATEMENT OF SIGNIFICANCE: A number of studies have shown that Zn effectively inhibits the growth and development of bacteria and yeast fungi.					
31499965	15	119	theme	Zn	2758:2759	arg1	ions					2761:2764	the establishing Zn ions	2741:2764	the establishing Zn ions' behavior	2741:2774	The present study provides data of the multiphase morphology, antimicrobial activity, biocompatibility and cytotoxicity of the biomimetic nanostructured composite materials, such as sodium alginate/hydroxyapatite/ZnO based granules and hydroxyapatite/ZnO based hydrogel, and the establishing Zn ions' behavior patterns with another composite components.					
31499965	1	120	theme	materials	327:335	arg1	biocompatibility					253:268	biocompatibility	253:268	biocompatibility	253:268	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	1	120	theme	materials	327:335	arg1	properties					288:297	antibacterial properties	274:297	antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride	274:616	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	6	121	theme	HA	1271:1272	arg1	lattice					1274:1280	the HA lattice	1267:1280	the HA lattice	1267:1280	At the same time, a partial replacement of Ca2+ ions by Zn2+ ions in the HA lattice possibly occurs due to processing of composite by US radiation.					
31499965	10	122	from	influence	1847:1855	arg1	properties					1885:1894	the biomimetic composite properties	1860:1894	the biomimetic composite properties	1860:1894	An interpretation of the process of Zn ions' embedding into hydroxyapatite and alginate matrix is given, as well as their influence on the biomimetic composite properties is discussed in details.					
31499965	10	123	theme	biomimetic	1864:1873	arg1	properties					1885:1894	the biomimetic composite properties	1860:1894	the biomimetic composite properties	1860:1894	An interpretation of the process of Zn ions' embedding into hydroxyapatite and alginate matrix is given, as well as their influence on the biomimetic composite properties is discussed in details.					
31499965	1	124	theme	hydrous	555:561	arg1	ammonia					563:569	hydrous ammonia	555:569	hydrous ammonia	555:569	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	5	125	theme	phase	1171:1175	arg1	transition					1177:1186	ZnO nanocrystals' phase transition	1153:1186	ZnO nanocrystals' phase transition	1153:1186	The possible explanation of ZnO nanocrystals' phase transition is given.					
31499965	8	126	theme	hydroxyapatite	1633:1646	arg1	alginate					1600:1607	sodium alginate	1593:1607	sodium alginate	1593:1607	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	8	126	theme	hydroxyapatite	1633:1646	arg1	powder					1623:1628	moistened powder	1613:1628	moistened powder	1613:1628	The studied composite demonstrate a pronounced antibacterial activity due to the incorporation of ZnO particles into sodium alginate and moistened powder of hydroxyapatite.					
31499965	14	127	theme	information	2404:2414	arg1	lack					2396:2399	lack	2396:2399	lack of information and the study's results about those interactions	2396:2463	Nowadays we have lack of information and the study's results about those interactions.					
31499965	1	128	theme	alginate-biphasic	380:396	arg1	oxide					403:407	hydroxyapatite‑sodium alginate-biphasic zinc oxide	358:407	hydroxyapatite‑sodium alginate-biphasic zinc oxide	358:407	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	1	128	theme	alginate-biphasic	380:396	arg1	HA-Alg-ZnO					346:355	HA-Alg-ZnO	346:355	HA-Alg-ZnO	346:355	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	9	129	theme	HA-ZnO	1663:1668	arg1	forms					1654:1658	Both forms	1649:1658	Both forms of HA-ZnO (suspension) and HA-Alg-ZnO (beads)	1649:1704	Both forms of HA-ZnO (suspension) and HA-Alg-ZnO (beads) are biocompatible.					
31499965	1	130	theme	nitrate	577:583	arg1	hexahydrate					585:595	zinc nitrate hexahydrate	572:595	zinc nitrate hexahydrate	572:595	The paper presents the results of studies of biocompatibility and antibacterial properties of multiphase nanocomposite materials based on HA-Alg-ZnO (hydroxyapatite‑sodium alginate-biphasic zinc oxide) and HA-ZnO (hydroxyapatite‑zinc oxide), which were synthesized from the analytically pure calcium nitrate tetrahydrate, ammonium hydrophosphate, hydrous ammonia, zinc nitrate hexahydrate and calcium chloride.					
31499965	14	131	contain	have	2391:2394	arg1	Nowadays					2379:2386	Nowadays	2379:2386	Nowadays	2379:2386	Nowadays we have lack of information and the study's results about those interactions.					
31499965	14	131	contain	have	2391:2394	arg2	lack					2396:2399	lack	2396:2399	lack of information and the study's results about those interactions	2396:2463	Nowadays we have lack of information and the study's results about those interactions.					
31499965	2	132	theme	"	845:845	arg1	methods					847:853	modified "agar diffusion" methods	821:853	modified "agar diffusion" methods	821:853	The samples' antimicrobial activity assessment was carried out on Gram-negative (E. coli, P. aeruginosa) and Gram-positive bacteria (S. aureus and S. epidermidis) test cultures by the co-incubation and modified "agar diffusion" methods.					
31499965	10	133	theme	Zn	1761:1762	arg1	ions					1764:1767	Zn ions	1761:1767	Zn ions' embedding into hydroxyapatite and alginate matrix	1761:1818	An interpretation of the process of Zn ions' embedding into hydroxyapatite and alginate matrix is given, as well as their influence on the biomimetic composite properties is discussed in details.					
30286133	7	0	from	component	1565:1573	arg1	brain					1616:1620	the brain	1612:1620	the brain	1612:1620	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	2	1	theme	tumor	638:642	arg1	microenvironment					644:659	the harsh tumor microenvironment	628:659	the harsh tumor microenvironment	628:659	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	1	2	theme	biochemical	380:390	arg1	cues					392:395	external biochemical cues	371:395	external biochemical cues in the microenvironment	371:419	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	8	3	theme	multi-scale	1902:1912	arg1	model					1927:1931	a multi-scale mathematical model	1900:1931	a multi-scale mathematical model	1900:1931	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	5	4	theme	glucose	1260:1266	arg1	levels					1268:1273	fluctuating glucose levels	1248:1273	fluctuating glucose levels	1248:1273	The model identifies a key mechanism underlying the molecular switches between proliferative phase and migratory phase in response to metabolic stress in response to fluctuating glucose levels.					
30286133	7	5	theme	astrocytes	1851:1860	arg1	dynamics					1757:1764	the dynamics	1753:1764	the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia	1753:1891	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	5	theme	astrocytes	1851:1860	arg1	status					1832:1837	the spatiotemporal activation status	1802:1837	the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia	1753:1891	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	2	6	from	stress	618:623	arg1	microenvironment					644:659	the harsh tumor microenvironment	628:659	the harsh tumor microenvironment	628:659	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	1	7	from	responses	315:323	arg1	response					359:366	response	359:366	response to external biochemical cues in the microenvironment	359:419	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	1	8	theme	migration	264:272	arg1	patterns					274:281	the migration patterns	260:281	the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment	260:419	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	7	9	from	matrix	1596:1601	arg1	brain					1616:1620	the brain	1612:1620	the brain	1612:1620	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	10	10	theme	main	2397:2400	arg1	lesion					2408:2413	the main tumor lesion	2393:2413	the main tumor lesion	2393:2413	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	7	11	theme	activation	1821:1830	arg1	status					1832:1837	the spatiotemporal activation status	1802:1837	the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia	1753:1891	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	9	12	theme	glycosylated	2233:2244	arg1	CSPGs					2246:2250	glycosylated CSPGs	2233:2250	glycosylated CSPGs	2233:2250	We show that the CSPG-rich microenvironment is associated with non-invasive tumor lesions through LAR-CSGAG binding while the absence of glycosylated CSPGs induce the critical glioma invasion.					
30286133	6	13	from	down-regulation	1295:1309	arg1	pathways					1334:1341	these pathways	1328:1341	these pathways	1328:1341	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	8	14	theme	stromal	2081:2087	arg1	cells					2089:2093	stromal cells	2081:2093	stromal cells	2081:2093	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	7	15	theme	chondroitin	1514:1524	arg1	component					1565:1573	a major component	1557:1573	a major component of the extracellular matrix (ECM) in the brain	1557:1620	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	15	theme	chondroitin	1514:1524	arg1	CSPGs					1549:1553	CSPGs	1549:1553	CSPGs	1549:1553	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	15	theme	chondroitin	1514:1524	arg1	proteoglycans					1534:1546	Glycosylated chondroitin sulfate proteoglycans	1501:1546	Glycosylated chondroitin sulfate proteoglycans (CSPGs)	1501:1554	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	2	16	theme	metabolic	608:616	arg1	stress					618:623	metabolic stress	608:623	metabolic stress in the harsh tumor microenvironment	608:659	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	3	17	theme	cells	789:793	arg1	inaccessibility					754:768	inaccessibility	754:768	inaccessibility of dispersed tumor cells in normal brain tissue	754:816	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	10	18	theme	local	2362:2366	arg1	astrocytes					2377:2386	local reactive astrocytes	2362:2386	local reactive astrocytes from the main tumor lesion	2362:2413	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	7	19	theme	microenvironment	1679:1694	arg1	structure					1650:1658	the physical structure	1637:1658	the physical structure of the local brain microenvironment	1637:1694	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	3	20	theme	dispersed	773:781	arg1	cells					789:793	dispersed tumor cells	773:793	dispersed tumor cells	773:793	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	12	21	theme	therapeutic	2763:2773	arg1	strategies					2775:2784	careful therapeutic strategies	2755:2784	careful therapeutic strategies	2755:2784	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	5	22	theme	key	1105:1107	arg1	mechanism					1109:1117	a key mechanism	1103:1117	a key mechanism underlying the molecular switches between proliferative phase and migratory phase in response to metabolic stress in response to fluctuating glucose levels	1103:1273	The model identifies a key mechanism underlying the molecular switches between proliferative phase and migratory phase in response to metabolic stress in response to fluctuating glucose levels.					
30286133	7	23	theme	local	1667:1671	arg1	microenvironment					1679:1694	the local brain microenvironment	1663:1694	the local brain microenvironment	1663:1694	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	8	24	theme	invasive	1979:1986	arg1	tumors					2005:2010	invasive and non-invasive tumors	1979:2010	invasive and non-invasive tumors	1979:2010	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	6	25	theme	stochastic	1483:1492	arg1	noise					1494:1498	stochastic noise	1483:1498	stochastic noise	1483:1498	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	10	26	theme	tumor	2402:2406	arg1	lesion					2408:2413	the main tumor lesion	2393:2413	the main tumor lesion	2393:2413	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	1	27	theme	primary	216:222	arg1	cancer					230:235	primary brain cancer	216:235	primary brain cancer	216:235	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	10	28	theme	non-invasive	2444:2455	arg1	tumor					2457:2461	non-invasive tumor	2444:2461	non-invasive tumor	2444:2461	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	8	29	from	coordination	2024:2035	arg1	cells					2089:2093	stromal cells	2081:2093	stromal cells	2081:2093	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	3	30	theme	normal	798:803	arg1	tissue					811:816	normal brain tissue	798:816	normal brain tissue	798:816	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	6	31	theme	cellular	1359:1366	arg1	decision					1368:1375	the key cellular decision	1351:1375	the key cellular decision to infiltrate	1351:1389	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	7	32	theme	receptor	1778:1785	arg1	LAR					1787:1789	the CSPG receptor LAR	1769:1789	the CSPG receptor LAR	1769:1789	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	33	theme	physical	1641:1648	arg1	structure					1650:1658	the physical structure	1637:1658	the physical structure of the local brain microenvironment	1637:1694	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	8	34	from	changes	2070:2076	arg1	cells					2089:2093	stromal cells	2081:2093	stromal cells	2081:2093	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	11	35	theme	CSPG	2513:2516	arg1	conditions					2518:2527	These different CSPG conditions	2497:2527	These different CSPG conditions	2497:2527	These different CSPG conditions also change the spatial profiles of ramified and activated microglia.					
30286133	12	36	theme	careful	2755:2761	arg1	strategies					2775:2784	careful therapeutic strategies	2755:2784	careful therapeutic strategies	2755:2784	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	12	37	theme	CSPGs	2627:2631	arg1	distribution					2611:2622	The complex distribution	2599:2622	The complex distribution of CSPGs in the tumor microenvironment	2599:2661	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	9	38	theme	critical	2263:2270	arg1	invasion					2279:2286	the critical glioma invasion	2259:2286	the critical glioma invasion	2259:2286	We show that the CSPG-rich microenvironment is associated with non-invasive tumor lesions through LAR-CSGAG binding while the absence of glycosylated CSPGs induce the critical glioma invasion.					
30286133	1	39	theme	individual	332:341	arg1	cells					350:354	the individual cancer cells	328:354	the individual cancer cells	328:354	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	4	40	dep	cell	863:866	arg1	invasion					886:893	invasion	886:893	invasion	886:893	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	4	40	dep	cell	863:866	arg1	proliferation					868:880	proliferation	868:880	proliferation	868:880	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	1	41	theme	therapeutic	151:161	arg1	control					163:169	therapeutic control	151:169	therapeutic control	151:169	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	4	42	theme	miR-451-AMPK-	1019:1031	arg1	pathway					1059:1065	the miR-451-AMPK- mTOR-cell cycle signaling pathway	1015:1065	the miR-451-AMPK- mTOR-cell cycle signaling pathway	1015:1065	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	11	43	theme	activated	2578:2586	arg1	microglia					2588:2596	ramified and activated microglia	2565:2596	microglia	2588:2596	These different CSPG conditions also change the spatial profiles of ramified and activated microglia.					
30286133	0	44	theme	growth	73:78	arg1	regulation					53:62	regulation	53:62	regulation of tumor growth and LAR-mediated invasion in glioblastoma	53:120	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.					
30286133	1	45	theme	cells	350:354	arg1	surgery					289:295	surgery	289:295	surgery	289:295	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	1	45	theme	cells	350:354	arg1	responses					315:323	intracellular responses	301:323	intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment	301:419	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	2	46	theme	rapid	560:564	arg1	proliferation					566:578	rapid proliferation	560:578	rapid proliferation	560:578	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	0	47	from	Role	0:3	arg1	regulation					53:62	regulation	53:62	regulation of tumor growth and LAR-mediated invasion in glioblastoma	53:120	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.					
30286133	10	48	theme	molecular	2312:2320	arg1	weight					2322:2327	high molecular weight	2307:2327	high molecular weight CSPGs	2307:2333	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	0	49	theme	LAR-mediated	84:95	arg1	invasion					97:104	LAR-mediated invasion	84:104	LAR-mediated invasion	84:104	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.					
30286133	5	50	theme	molecular	1134:1142	arg1	switches					1144:1151	the molecular switches	1130:1151	the molecular switches between proliferative phase and migratory phase	1130:1199	The model identifies a key mechanism underlying the molecular switches between proliferative phase and migratory phase in response to metabolic stress in response to fluctuating glucose levels.					
30286133	8	51	theme	CSPG-induced	1951:1962	arg1	switch					1964:1969	a CSPG-induced switch	1949:1969	a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells	1949:2093	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	4	52	theme	intracellular	989:1001	arg1	dynamics					1003:1010	the intracellular dynamics	985:1010	the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell	985:1079	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	12	53	theme	glioma	2713:2718	arg1	cells					2720:2724	glioma cells	2713:2724	glioma cells	2713:2724	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	1	54	theme	external	371:378	arg1	cues					392:395	external biochemical cues	371:395	external biochemical cues in the microenvironment	371:419	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	0	55	theme	microenvironment	33:48	arg1	Role					0:3	Role	0:3	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.	0:121	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.					
30286133	6	56	theme	noise	1494:1498	arg1	presence					1455:1462	presence	1455:1462	presence	1455:1462	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	6	56	theme	noise	1494:1498	arg1	absence					1443:1449	absence	1443:1449	absence	1443:1449	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	6	57	dep	absence	1443:1449	arg1	the					1439:1441	the	1439:1441	the	1439:1441	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	6	58	theme	components	1314:1323	arg1	down-regulation					1295:1309	down-regulation	1295:1309	down-regulation	1295:1309	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	6	58	theme	components	1314:1323	arg1	up-					1288:1290	up-	1288:1290	up-	1288:1290	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	10	59	from	exodus	2352:2357	arg1	lesion					2408:2413	the main tumor lesion	2393:2413	the main tumor lesion	2393:2413	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	9	60	theme	CSPG-rich	2113:2121	arg1	microenvironment					2123:2138	the CSPG-rich microenvironment	2109:2138	the CSPG-rich microenvironment	2109:2138	We show that the CSPG-rich microenvironment is associated with non-invasive tumor lesions through LAR-CSGAG binding while the absence of glycosylated CSPGs induce the critical glioma invasion.					
30286133	0	61	theme	extracellular	8:20	arg1	matrix					22:27	matrix	22:27	matrix	22:27	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.					
30286133	7	62	theme	matrix	1596:1601	arg1	component					1565:1573	a major component	1557:1573	a major component of the extracellular matrix (ECM) in the brain	1557:1620	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	62	theme	matrix	1596:1601	arg1	proteoglycans					1534:1546	Glycosylated chondroitin sulfate proteoglycans	1501:1546	Glycosylated chondroitin sulfate proteoglycans (CSPGs)	1501:1554	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	4	63	theme	cycle	1043:1047	arg1	pathway					1059:1065	the miR-451-AMPK- mTOR-cell cycle signaling pathway	1015:1065	the miR-451-AMPK- mTOR-cell cycle signaling pathway	1015:1065	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	3	64	theme	tumor	691:695	arg1	removal					671:677	Surgical removal	662:677	Surgical removal of the main tumor	662:695	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	4	65	theme	complex	844:850	arg1	process					852:858	this complex process	839:858	this complex process of cell proliferation and invasion	839:893	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	2	66	theme	Recent	422:427	arg1	studies					429:435	Recent studies	422:435	Recent studies	422:435	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	6	67	theme	time	1467:1470	arg1	delays					1472:1477	time delays	1467:1477	time delays	1467:1477	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	1	68	theme	intracellular	301:313	arg1	responses					315:323	intracellular responses	301:323	intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment	301:419	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	4	69	theme	pathway	1059:1065	arg1	dynamics					1003:1010	the intracellular dynamics	985:1010	the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell	985:1079	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	7	70	theme	microglia	1883:1891	arg1	dynamics					1757:1764	the dynamics	1753:1764	the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia	1753:1891	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	70	theme	microglia	1883:1891	arg1	status					1832:1837	the spatiotemporal activation status	1802:1837	the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia	1753:1891	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	12	71	theme	tumor	2640:2644	arg1	microenvironment					2646:2661	the tumor microenvironment	2636:2661	the tumor microenvironment	2636:2661	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	9	72	theme	tumor	2172:2176	arg1	lesions					2178:2184	non-invasive tumor lesions	2159:2184	non-invasive tumor lesions	2159:2184	We show that the CSPG-rich microenvironment is associated with non-invasive tumor lesions through LAR-CSGAG binding while the absence of glycosylated CSPGs induce the critical glioma invasion.					
30286133	1	73	theme	brain	224:228	arg1	cancer					230:235	primary brain cancer	216:235	primary brain cancer	216:235	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	2	74	from	proliferation	566:578	arg1	response					596:603	response	596:603	response to metabolic stress in the harsh tumor microenvironment	596:659	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	12	75	theme	nonlinear	2681:2689	arg1	behaviors					2700:2708	the nonlinear invasion behaviors	2677:2708	the nonlinear invasion behaviors	2677:2708	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	12	76	from	distribution	2611:2622	arg1	microenvironment					2646:2661	the tumor microenvironment	2636:2661	the tumor microenvironment	2636:2661	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	7	77	theme	resident	1842:1849	arg1	astrocytes					1851:1860	resident astrocytes	1842:1860	resident astrocytes	1842:1860	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	2	78	from	invasion	584:591	arg1	response					596:603	response	596:603	response to metabolic stress in the harsh tumor microenvironment	596:659	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	3	79	theme	tumor	741:745	arg1	recurrence					723:732	recurrence	723:732	recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue	723:816	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	8	80	theme	mathematical	1914:1925	arg1	model					1927:1931	a multi-scale mathematical model	1900:1931	a multi-scale mathematical model	1900:1931	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	9	81	theme	CSPGs	2246:2250	arg1	absence					2222:2228	the absence	2218:2228	the absence of glycosylated CSPGs	2218:2250	We show that the CSPG-rich microenvironment is associated with non-invasive tumor lesions through LAR-CSGAG binding while the absence of glycosylated CSPGs induce the critical glioma invasion.					
30286133	5	82	theme	fluctuating	1248:1258	arg1	levels					1268:1273	fluctuating glucose levels	1248:1273	fluctuating glucose levels	1248:1273	The model identifies a key mechanism underlying the molecular switches between proliferative phase and migratory phase in response to metabolic stress in response to fluctuating glucose levels.					
30286133	2	83	theme	harsh	632:636	arg1	microenvironment					644:659	the harsh tumor microenvironment	628:659	the harsh tumor microenvironment	628:659	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	9	84	gly	glycosylated	2233:2244	arg1	CSPGs					2246:2250	glycosylated CSPGs	2233:2250	glycosylated CSPGs	2233:2250	We show that the CSPG-rich microenvironment is associated with non-invasive tumor lesions through LAR-CSGAG binding while the absence of glycosylated CSPGs induce the critical glioma invasion.					
30286133	9	85	theme	glioma	2272:2277	arg1	invasion					2279:2286	the critical glioma invasion	2259:2286	the critical glioma invasion	2259:2286	We show that the CSPG-rich microenvironment is associated with non-invasive tumor lesions through LAR-CSGAG binding while the absence of glycosylated CSPGs induce the critical glioma invasion.					
30286133	7	86	theme	sulfate	1526:1532	arg1	component					1565:1573	a major component	1557:1573	a major component of the extracellular matrix (ECM) in the brain	1557:1620	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	86	theme	sulfate	1526:1532	arg1	CSPGs					1549:1553	CSPGs	1549:1553	CSPGs	1549:1553	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	86	theme	sulfate	1526:1532	arg1	proteoglycans					1534:1546	Glycosylated chondroitin sulfate proteoglycans	1501:1546	Glycosylated chondroitin sulfate proteoglycans (CSPGs)	1501:1554	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	87	theme	spatiotemporal	1806:1819	arg1	status					1832:1837	the spatiotemporal activation status	1802:1837	the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia	1753:1891	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	1	88	from	surgery	289:295	arg1	response					359:366	response	359:366	response to external biochemical cues in the microenvironment	359:419	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	1	89	theme	glioblastoma	174:185	arg1	dispersion					136:145	cellular dispersion	127:145	cellular dispersion	127:145	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	1	89	theme	glioblastoma	174:185	arg1	control					163:169	therapeutic control	151:169	therapeutic control	151:169	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	2	90	theme	downstream	471:480	arg1	molecules					482:490	downstream molecules	471:490	downstream molecules including AMPK/CAB39/MARK and mTOR	471:525	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	2	90	theme	downstream	471:480	arg1	mTOR					522:525	mTOR	522:525	mTOR	522:525	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	2	90	theme	downstream	471:480	arg1	AMPK/CAB39/MARK					502:516	AMPK/CAB39/MARK	502:516	AMPK/CAB39/MARK	502:516	Recent studies have shown that miR-451 regulates downstream molecules including AMPK/CAB39/MARK and mTOR to determine the balance between rapid proliferation and invasion in response to metabolic stress in the harsh tumor microenvironment.					
30286133	7	91	theme	Glycosylated	1501:1512	arg1	component					1565:1573	a major component	1557:1573	a major component of the extracellular matrix (ECM) in the brain	1557:1620	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	91	theme	Glycosylated	1501:1512	arg1	CSPGs					1549:1553	CSPGs	1549:1553	CSPGs	1549:1553	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	91	theme	Glycosylated	1501:1512	arg1	proteoglycans					1534:1546	Glycosylated chondroitin sulfate proteoglycans	1501:1546	Glycosylated chondroitin sulfate proteoglycans (CSPGs)	1501:1554	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	4	92	theme	cell	863:866	arg1	response					903:910	its response	899:910	its response to conventional treatment	899:936	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	4	92	theme	cell	863:866	arg1	process					852:858	this complex process	839:858	this complex process of cell proliferation and invasion	839:893	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	12	93	theme	complex	2603:2609	arg1	distribution					2611:2622	The complex distribution	2599:2622	The complex distribution of CSPGs in the tumor microenvironment	2599:2661	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	8	94	theme	ECM-cell	2040:2047	arg1	adhesion					2049:2056	ECM-cell adhesion	2040:2056	ECM-cell adhesion	2040:2056	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	5	95	from	stress	1226:1231	arg1	response					1236:1243	response	1236:1243	response to fluctuating glucose levels	1236:1273	The model identifies a key mechanism underlying the molecular switches between proliferative phase and migratory phase in response to metabolic stress in response to fluctuating glucose levels.					
30286133	5	96	theme	migratory	1185:1193	arg1	phase					1195:1199	migratory phase	1185:1199	migratory phase	1185:1199	The model identifies a key mechanism underlying the molecular switches between proliferative phase and migratory phase in response to metabolic stress in response to fluctuating glucose levels.					
30286133	7	97	theme	brain	1673:1677	arg1	microenvironment					1679:1694	the local brain microenvironment	1663:1694	the local brain microenvironment	1663:1694	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	10	98	theme	astrocytes	2377:2386	arg1	exodus					2352:2357	the exodus	2348:2357	the exodus of local reactive astrocytes from the main tumor lesion	2348:2413	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	3	99	from	inaccessibility	754:768	arg1	tissue					811:816	normal brain tissue	798:816	normal brain tissue	798:816	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	1	100	theme	aggressive	197:206	arg1	type					208:211	the most aggressive type	188:211	the most aggressive type of primary brain cancer	188:235	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	1	100	theme	aggressive	197:206	arg1	glioblastoma					174:185	glioblastoma	174:185	glioblastoma	174:185	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	3	101	theme	tumor	783:787	arg1	cells					789:793	dispersed tumor cells	773:793	dispersed tumor cells	773:793	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	4	102	theme	mathematical	952:963	arg1	model					965:969	a mathematical model	950:969	a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell	950:1079	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	8	103	theme	dynamic	2062:2068	arg1	changes					2070:2076	dynamic changes	2062:2076	dynamic changes in stromal cells	2062:2093	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	11	104	theme	ramified	2565:2572	arg1	microglia					2588:2596	ramified and activated microglia	2565:2596	microglia	2588:2596	These different CSPG conditions also change the spatial profiles of ramified and activated microglia.					
30286133	4	105	theme	conventional	915:926	arg1	treatment					928:936	conventional treatment	915:936	conventional treatment	915:936	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	11	106	theme	different	2503:2511	arg1	conditions					2518:2527	These different CSPG conditions	2497:2527	These different CSPG conditions	2497:2527	These different CSPG conditions also change the spatial profiles of ramified and activated microglia.					
30286133	3	107	theme	brain	805:809	arg1	tissue					811:816	normal brain tissue	798:816	normal brain tissue	798:816	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	6	108	theme	key	1355:1357	arg1	decision					1368:1375	the key cellular decision	1351:1375	the key cellular decision to infiltrate	1351:1389	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	3	109	theme	Surgical	662:669	arg1	removal					671:677	Surgical removal	662:677	Surgical removal of the main tumor	662:695	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	1	110	theme	cellular	127:134	arg1	dispersion					136:145	cellular dispersion	127:145	cellular dispersion	127:145	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	7	111	theme	LAR	1787:1789	arg1	dynamics					1757:1764	the dynamics	1753:1764	the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia	1753:1891	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	111	theme	LAR	1787:1789	arg1	status					1832:1837	the spatiotemporal activation status	1802:1837	the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia	1753:1891	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	11	112	theme	spatial	2545:2551	arg1	profiles					2553:2560	the spatial profiles	2541:2560	the spatial profiles of ramified and activated microglia	2541:2596	These different CSPG conditions also change the spatial profiles of ramified and activated microglia.					
30286133	6	113	from	up-	1288:1290	arg1	pathways					1334:1341	these pathways	1328:1341	these pathways	1328:1341	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	0	114	theme	tumor	67:71	arg1	growth					73:78	tumor growth	67:78	tumor growth	67:78	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.					
30286133	7	115	theme	CSPG	1773:1776	arg1	LAR					1787:1789	the CSPG receptor LAR	1769:1789	the CSPG receptor LAR	1769:1789	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	6	116	theme	complex	1411:1417	arg1	microenvironment					1419:1434	a complex microenvironment	1409:1434	a complex microenvironment in the absence and presence of time delays and stochastic noise	1409:1498	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	8	117	theme	adhesion	2049:2056	arg1	coordination					2024:2035	the coordination	2020:2035	the coordination of ECM-cell adhesion	2020:2056	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	8	117	theme	adhesion	2049:2056	arg1	changes					2070:2076	dynamic changes	2062:2076	dynamic changes in stromal cells	2062:2093	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	1	118	theme	cancer	343:348	arg1	cells					350:354	the individual cancer cells	328:354	the individual cancer cells	328:354	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	8	119	theme	non-invasive	1992:2003	arg1	tumors					2005:2010	invasive and non-invasive tumors	1979:2010	invasive and non-invasive tumors	1979:2010	Using a multi-scale mathematical model, we investigate a CSPG-induced switch between invasive and non-invasive tumors through the coordination of ECM-cell adhesion and dynamic changes in stromal cells.					
30286133	10	120	theme	tumor	2457:2461	arg1	inhibition					2467:2476	inhibition	2467:2476	inhibition of tumor invasion	2467:2494	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	10	120	theme	tumor	2457:2461	arg1	encapsulation					2427:2439	encapsulation	2427:2439	encapsulation of non-invasive tumor	2427:2461	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	10	121	theme	weight	2322:2327	arg1	CSPGs					2329:2333	high molecular weight CSPGs	2307:2333	high molecular weight CSPGs	2307:2333	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	0	122	theme	invasion	97:104	arg1	regulation					53:62	regulation	53:62	regulation of tumor growth and LAR-mediated invasion in glioblastoma	53:120	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.					
30286133	12	123	theme	cells	2720:2724	arg1	behaviors					2700:2708	the nonlinear invasion behaviors	2677:2708	the nonlinear invasion behaviors	2677:2708	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	0	124	from	regulation	53:62	arg1	glioblastoma					109:120	glioblastoma	109:120	glioblastoma	109:120	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.					
30286133	10	125	theme	high	2307:2310	arg1	weight					2322:2327	high molecular weight	2307:2327	high molecular weight CSPGs	2307:2333	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	1	126	dep	dispersion	136:145	arg1	The					123:125	The	123:125	The	123:125	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	1	127	from	cues	392:395	arg1	microenvironment					404:419	the microenvironment	400:419	the microenvironment	400:419	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	10	128	theme	tumor	2481:2485	arg1	invasion					2487:2494	tumor invasion	2481:2494	tumor invasion	2481:2494	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	11	129	theme	microglia	2588:2596	arg1	profiles					2553:2560	the spatial profiles	2541:2560	the spatial profiles of ramified and activated microglia	2541:2596	These different CSPG conditions also change the spatial profiles of ramified and activated microglia.					
30286133	10	130	theme	invasion	2487:2494	arg1	inhibition					2467:2476	inhibition	2467:2476	inhibition of tumor invasion	2467:2494	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	10	130	theme	invasion	2487:2494	arg1	encapsulation					2427:2439	encapsulation	2427:2439	encapsulation of non-invasive tumor	2427:2461	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	5	131	theme	proliferative	1161:1173	arg1	phase					1175:1179	proliferative phase	1161:1179	proliferative phase	1161:1179	The model identifies a key mechanism underlying the molecular switches between proliferative phase and migratory phase in response to metabolic stress in response to fluctuating glucose levels.					
30286133	7	132	theme	glioma	1723:1728	arg1	invasion					1730:1737	glioma invasion	1723:1737	glioma invasion	1723:1737	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	10	133	theme	reactive	2368:2375	arg1	astrocytes					2377:2386	local reactive astrocytes	2362:2386	local reactive astrocytes from the main tumor lesion	2362:2413	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	12	134	theme	invasion	2691:2698	arg1	behaviors					2700:2708	the nonlinear invasion behaviors	2677:2708	the nonlinear invasion behaviors	2677:2708	The complex distribution of CSPGs in the tumor microenvironment can determine the nonlinear invasion behaviors of glioma cells, which suggests the need for careful therapeutic strategies.					
30286133	0	135	theme	matrix	22:27	arg1	Role					0:3	Role	0:3	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.	0:121	Role of extracellular matrix and microenvironment in regulation of tumor growth and LAR-mediated invasion in glioblastoma.					
30286133	3	136	theme	main	686:689	arg1	tumor					691:695	the main tumor	682:695	the main tumor	682:695	Surgical removal of the main tumor is inevitably followed by recurrence of the tumor due to inaccessibility of dispersed tumor cells in normal brain tissue.					
30286133	6	137	theme	delays	1472:1477	arg1	presence					1455:1462	presence	1455:1462	presence	1455:1462	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	6	137	theme	delays	1472:1477	arg1	absence					1443:1449	absence	1443:1449	absence	1443:1449	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	4	138	theme	mTOR-cell	1033:1041	arg1	pathway					1059:1065	the miR-451-AMPK- mTOR-cell cycle signaling pathway	1015:1065	the miR-451-AMPK- mTOR-cell cycle signaling pathway	1015:1065	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	7	139	from	brain	1616:1620	arg1	component					1565:1573	a major component	1557:1573	a major component of the extracellular matrix (ECM) in the brain	1557:1620	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	139	from	brain	1616:1620	arg1	proteoglycans					1534:1546	Glycosylated chondroitin sulfate proteoglycans	1501:1546	Glycosylated chondroitin sulfate proteoglycans (CSPGs)	1501:1554	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	140	theme	extracellular	1582:1594	arg1	ECM					1604:1606	ECM	1604:1606	ECM	1604:1606	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	140	theme	extracellular	1582:1594	arg1	matrix					1596:1601	the extracellular matrix	1578:1601	the extracellular matrix (ECM) in the brain	1578:1620	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	6	141	from	microenvironment	1419:1434	arg1	presence					1455:1462	presence	1455:1462	presence	1455:1462	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	6	141	from	microenvironment	1419:1434	arg1	absence					1443:1449	absence	1443:1449	absence	1443:1449	We show how up- or down-regulation of components in these pathways affects the key cellular decision to infiltrate or proliferate in a complex microenvironment in the absence and presence of time delays and stochastic noise.					
30286133	4	142	theme	signaling	1049:1057	arg1	pathway					1059:1065	the miR-451-AMPK- mTOR-cell cycle signaling pathway	1015:1065	the miR-451-AMPK- mTOR-cell cycle signaling pathway	1015:1065	In order to address this complex process of cell proliferation and invasion and its response to conventional treatment, we propose a mathematical model that analyzes the intracellular dynamics of the miR-451-AMPK- mTOR-cell cycle signaling pathway within a cell.					
30286133	9	143	theme	non-invasive	2159:2170	arg1	lesions					2178:2184	non-invasive tumor lesions	2159:2184	non-invasive tumor lesions	2159:2184	We show that the CSPG-rich microenvironment is associated with non-invasive tumor lesions through LAR-CSGAG binding while the absence of glycosylated CSPGs induce the critical glioma invasion.					
30286133	5	144	theme	metabolic	1216:1224	arg1	stress					1226:1231	metabolic stress	1216:1231	metabolic stress in response to fluctuating glucose levels	1216:1273	The model identifies a key mechanism underlying the molecular switches between proliferative phase and migratory phase in response to metabolic stress in response to fluctuating glucose levels.					
30286133	10	145	from	lesion	2408:2413	arg1	exodus					2352:2357	the exodus	2348:2357	the exodus of local reactive astrocytes from the main tumor lesion	2348:2413	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	10	145	from	lesion	2408:2413	arg1	astrocytes					2377:2386	local reactive astrocytes	2362:2386	local reactive astrocytes from the main tumor lesion	2362:2413	We illustrate how high molecular weight CSPGs can regulate the exodus of local reactive astrocytes from the main tumor lesion, leading to encapsulation of non-invasive tumor and inhibition of tumor invasion.					
30286133	1	146	theme	cancer	230:235	arg1	type					208:211	the most aggressive type	188:211	the most aggressive type of primary brain cancer	188:235	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	1	146	theme	cancer	230:235	arg1	glioblastoma					174:185	glioblastoma	174:185	glioblastoma	174:185	The cellular dispersion and therapeutic control of glioblastoma, the most aggressive type of primary brain cancer, depends critically on the migration patterns after surgery and intracellular responses of the individual cancer cells in response to external biochemical cues in the microenvironment.					
30286133	7	147	theme	major	1559:1563	arg1	component					1565:1573	a major component	1557:1573	a major component of the extracellular matrix (ECM) in the brain	1557:1620	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	147	theme	major	1559:1563	arg1	proteoglycans					1534:1546	Glycosylated chondroitin sulfate proteoglycans	1501:1546	Glycosylated chondroitin sulfate proteoglycans (CSPGs)	1501:1554	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	7	148	theme	tumor-associated	1866:1881	arg1	microglia					1883:1891	tumor-associated microglia	1866:1891	tumor-associated microglia	1866:1891	Glycosylated chondroitin sulfate proteoglycans (CSPGs), a major component of the extracellular matrix (ECM) in the brain, contribute to the physical structure of the local brain microenvironment but also induce or inhibit glioma invasion by regulating the dynamics of the CSPG receptor LAR as well as the spatiotemporal activation status of resident astrocytes and tumor-associated microglia.					
30286133	9	149	theme	LAR-CSGAG	2194:2202	arg1	binding					2204:2210	LAR-CSGAG binding	2194:2210	LAR-CSGAG binding	2194:2210	We show that the CSPG-rich microenvironment is associated with non-invasive tumor lesions through LAR-CSGAG binding while the absence of glycosylated CSPGs induce the critical glioma invasion.					
31870866	0	0	theme	vessel	91:96	arg1	constructs					98:107	vessel constructs	91:107	vessel constructs	91:107	Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs.					
31870866	1	1	theme	structural	151:160	arg1	reconstruction					162:175	its high structural reconstruction	142:175	its high structural reconstruction	142:175	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	7	2	theme	nanotubes	1365:1373	arg1	doping					1348:1353	doping	1348:1353	doping of carbon nanotubes	1348:1373	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	3	3	theme	motor	839:843	arg1	module					845:850	stepper motor module	831:850	stepper motor module	831:850	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	5	4	theme	7-10 cm	1133:1139	arg1	length					1123:1128	a length	1121:1128	a length of 7-10 cm	1121:1139	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	5	4	theme	7-10 cm	1133:1139	arg1	thickness					1097:1105	an average wall thickness	1081:1105	an average wall thickness of 0.5 mm	1081:1115	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	6	5	theme	composite	1266:1274	arg1	scaffolds					1276:1284	the composite scaffolds	1262:1284	the composite scaffolds	1262:1284	Results demonstrated that the proper doping of carbon nanotubes could effectively increase the mechanical properties of the composite scaffolds.					
31870866	2	6	theme	large-sized	341:351	arg1	vessels					359:365	large-sized blood vessels	341:365	large-sized blood vessels in the human body	341:383	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	3	7	theme	module	845:850	arg1	nozzle					802:807	printing nozzle	793:807	printing nozzle	793:807	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	3	7	theme	module	845:850	arg1	rotation					819:826	axial rotation	813:826	axial rotation of stepper motor module	813:850	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	6	8	theme	proper	1172:1177	arg1	doping					1179:1184	the proper doping	1168:1184	the proper doping of carbon nanotubes	1168:1204	Results demonstrated that the proper doping of carbon nanotubes could effectively increase the mechanical properties of the composite scaffolds.					
31870866	6	9	theme	carbon	1189:1194	arg1	nanotubes					1196:1204	carbon nanotubes	1189:1204	carbon nanotubes	1189:1204	Results demonstrated that the proper doping of carbon nanotubes could effectively increase the mechanical properties of the composite scaffolds.					
31870866	4	10	theme	engineered	969:978	arg1	vessels					986:992	engineered blood vessels	969:992	engineered blood vessels	969:992	Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels.					
31870866	0	11	theme	constructs	98:107	arg1	printing					79:86	direct 3D printing	69:86	direct 3D printing of vessel constructs	69:107	Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs.					
31870866	2	12	theme	blood	353:357	arg1	vessels					359:365	large-sized blood vessels	341:365	large-sized blood vessels in the human body	341:383	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	5	13	with	3 mm	1071:1074	arg1	length					1123:1128	a length	1121:1128	a length of 7-10 cm	1121:1139	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	5	13	with	3 mm	1071:1074	arg1	thickness					1097:1105	an average wall thickness	1081:1105	an average wall thickness of 0.5 mm	1081:1115	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	3	14	theme	stepper	831:837	arg1	module					845:850	stepper motor module	831:850	stepper motor module	831:850	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	2	15	theme	microvascular	481:493	arg1	simulation					495:504	the unfulfilled microvascular simulation	465:504	the unfulfilled microvascular simulation	465:504	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	5	16	theme	0.5 mm	1110:1115	arg1	length					1123:1128	a length	1121:1128	a length of 7-10 cm	1121:1139	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	5	16	theme	0.5 mm	1110:1115	arg1	thickness					1097:1105	an average wall thickness	1081:1105	an average wall thickness of 0.5 mm	1081:1115	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	4	17	theme	Mouse	853:857	arg1	fibroblasts					869:879	Mouse epidermal fibroblasts	853:879	Mouse epidermal fibroblasts	853:879	Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels.					
31870866	2	18	from	printing	329:336	arg1	body					380:383	the human body	370:383	the human body	370:383	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	7	19	theme	doping	1348:1353	arg1	doping					1348:1353	doping	1348:1353	doping of carbon nanotubes	1348:1373	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	7	19	theme	doping	1348:1353	arg1	amount					1338:1343	a small amount	1330:1343	a small amount of doping of carbon nanotubes	1330:1373	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	2	20	theme	unfulfilled	469:479	arg1	simulation					495:504	the unfulfilled microvascular simulation	465:504	the unfulfilled microvascular simulation	465:504	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	3	21	theme	cylindrical	699:709	arg1	scaffolds					711:719	cylindrical scaffolds	699:719	cylindrical scaffolds	699:719	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	6	22	theme	scaffolds	1276:1284	arg1	properties					1248:1257	the mechanical properties	1233:1257	the mechanical properties of the composite scaffolds	1233:1284	Results demonstrated that the proper doping of carbon nanotubes could effectively increase the mechanical properties of the composite scaffolds.					
31870866	3	23	theme	rotation	819:826	arg1	extrusion					780:788	the vertical directional extrusion	755:788	the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module	755:850	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	1	24	theme	low	181:183	arg1	cost					185:188	low cost	181:188	low cost	181:188	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	0	25	theme	gelatin-based	12:24	arg1	alginate/carbon					26:40	Engineering gelatin-based alginate/carbon	0:40	Engineering gelatin-based alginate/carbon	0:40	Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs.					
31870866	7	26	theme	vascular	1471:1478	arg1	requirements					1444:1455	the requirements	1440:1455	the requirements of biomimetic vascular	1440:1478	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	2	27	dep	problems	425:432	arg1	solved					440:445	solved	440:445	to be solved urgently	434:454	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	4	28	theme	inner	906:910	arg1	walls					922:926	the inner and outer walls	902:926	the inner and outer walls of hollow tubular scaffolds	902:954	Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels.					
31870866	2	29	theme	human	374:378	arg1	body					380:383	the human body	370:383	the human body	370:383	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	0	30	theme	Engineering	0:10	arg1	alginate/carbon					26:40	Engineering gelatin-based alginate/carbon	0:40	Engineering gelatin-based alginate/carbon	0:40	Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs.					
31870866	1	31	theme	vascular	266:273	arg1	diseases					275:282	vascular diseases	266:282	vascular diseases	266:282	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	4	32	theme	epidermal	859:867	arg1	fibroblasts					869:879	Mouse epidermal fibroblasts	853:879	Mouse epidermal fibroblasts	853:879	Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels.					
31870866	5	33	theme	internal	999:1006	arg1	3 mm					1071:1074	3 mm	1071:1074	3 mm	1071:1074	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	5	33	theme	internal	999:1006	arg1	diameters					1008:1016	The internal diameters	995:1016	The internal diameters of the bionic circular tubes printed in batches	995:1064	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	1	34	theme	diseases	275:282	arg1	treatment					253:261	the treatment	249:261	the treatment of vascular diseases	249:282	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	2	35	theme	mechanical	540:549	arg1	strength					551:558	mechanical strength	540:558	mechanical strength	540:558	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	2	36	from	vessels	359:365	arg1	body					380:383	the human body	370:383	the human body	370:383	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	1	37	theme	Nowadays	110:117	arg1	bioprinting					122:132	Nowadays 3D bioprinting	110:132	Nowadays 3D bioprinting	110:132	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	1	37	theme	Nowadays	110:117	arg1	technology					212:221	a promising technology	200:221	a promising technology	200:221	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	1	38	theme	3D	119:120	arg1	bioprinting					122:132	Nowadays 3D bioprinting	110:132	Nowadays 3D bioprinting	110:132	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	1	38	theme	3D	119:120	arg1	technology					212:221	a promising technology	200:221	a promising technology	200:221	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	7	39	theme	small	1332:1336	arg1	doping					1348:1353	doping	1348:1353	doping of carbon nanotubes	1348:1373	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	7	39	theme	small	1332:1336	arg1	amount					1338:1343	a small amount	1330:1343	a small amount of doping of carbon nanotubes	1330:1373	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	4	40	theme	scaffolds	946:954	arg1	walls					922:926	the inner and outer walls	902:926	the inner and outer walls of hollow tubular scaffolds	902:954	Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels.					
31870866	5	41	theme	average	1084:1090	arg1	thickness					1097:1105	an average wall thickness	1081:1105	an average wall thickness of 0.5 mm	1081:1115	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	5	42	theme	circular	1032:1039	arg1	tubes					1041:1045	the bionic circular tubes	1021:1045	the bionic circular tubes printed in batches	1021:1064	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	0	43	theme	blend	52:56	arg1	bioink					58:63	blend bioink	52:63	blend bioink for direct 3D printing of vessel constructs	52:107	Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs.					
31870866	2	44	theme	inferior	510:517	arg1	biocompatibility					519:534	inferior biocompatibility	510:534	inferior biocompatibility	510:534	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	5	45	theme	wall	1092:1095	arg1	thickness					1097:1105	an average wall thickness	1081:1105	an average wall thickness of 0.5 mm	1081:1115	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	5	46	theme	tubes	1041:1045	arg1	3 mm					1071:1074	3 mm	1071:1074	3 mm	1071:1074	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	5	46	theme	tubes	1041:1045	arg1	diameters					1008:1016	The internal diameters	995:1016	The internal diameters of the bionic circular tubes printed in batches	995:1064	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	1	47	theme	promising	202:210	arg1	technology					212:221	a promising technology	200:221	a promising technology	200:221	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	1	47	theme	promising	202:210	arg1	bioprinting					122:132	Nowadays 3D bioprinting	110:132	Nowadays 3D bioprinting	110:132	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	0	48	theme	direct	69:74	arg1	printing					79:86	direct 3D printing	69:86	direct 3D printing of vessel constructs	69:107	Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs.					
31870866	3	49	theme	printing	793:800	arg1	nozzle					802:807	printing nozzle	793:807	printing nozzle	793:807	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	2	50	theme	materials	572:580	arg1	simulation					495:504	the unfulfilled microvascular simulation	465:504	the unfulfilled microvascular simulation	465:504	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	2	50	theme	materials	572:580	arg1	biocompatibility					519:534	inferior biocompatibility	510:534	inferior biocompatibility	510:534	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	2	50	theme	materials	572:580	arg1	strength					551:558	mechanical strength	540:558	mechanical strength	540:558	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	3	51	theme	vertical	759:766	arg1	extrusion					780:788	the vertical directional extrusion	755:788	the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module	755:850	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	6	52	theme	mechanical	1237:1246	arg1	properties					1248:1257	the mechanical properties	1233:1257	the mechanical properties of the composite scaffolds	1233:1284	Results demonstrated that the proper doping of carbon nanotubes could effectively increase the mechanical properties of the composite scaffolds.					
31870866	7	53	theme	quantitative	1293:1304	arg1	experiments					1306:1316	quantitative experiments	1293:1316	quantitative experiments	1293:1316	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	4	54	theme	tubular	938:944	arg1	scaffolds					946:954	hollow tubular scaffolds	931:954	hollow tubular scaffolds	931:954	Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels.					
31870866	3	55	theme	directional	768:778	arg1	extrusion					780:788	the vertical directional extrusion	755:788	the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module	755:850	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	2	56	theme	scaffold	563:570	arg1	materials					572:580	scaffold materials	563:580	scaffold materials	563:580	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	4	57	theme	blood	980:984	arg1	vessels					986:992	engineered blood vessels	969:992	engineered blood vessels	969:992	Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels.					
31870866	6	58	theme	nanotubes	1196:1204	arg1	doping					1179:1184	the proper doping	1168:1184	the proper doping of carbon nanotubes	1168:1204	Results demonstrated that the proper doping of carbon nanotubes could effectively increase the mechanical properties of the composite scaffolds.					
31870866	3	59	theme	carbon	659:664	arg1	nanotubes					666:674	carbon nanotubes	659:674	carbon nanotubes	659:674	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	3	60	theme	hybrid	602:607	arg1	bioink					609:614	the hybrid bioink	598:614	the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes	598:674	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	2	61	from	body	380:383	arg1	printing					329:336	3D printing	326:336	3D printing of large-sized blood vessels in the human body	326:383	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	1	62	theme	due	135:137	arg1	bioprinting					122:132	Nowadays 3D bioprinting	110:132	Nowadays 3D bioprinting	110:132	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	1	62	theme	due	135:137	arg1	technology					212:221	a promising technology	200:221	a promising technology	200:221	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	3	63	theme	axial	813:817	arg1	rotation					819:826	axial rotation	813:826	axial rotation of stepper motor module	813:850	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	4	64	theme	outer	916:920	arg1	walls					922:926	the inner and outer walls	902:926	the inner and outer walls of hollow tubular scaffolds	902:954	Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels.					
31870866	3	65	theme	sodium	639:644	arg1	alginate					646:653	sodium alginate	639:653	sodium alginate	639:653	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	7	66	theme	little	1379:1384	arg1	effect					1386:1391	little effect	1379:1391	little effect	1379:1391	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	1	67	theme	high	146:149	arg1	reconstruction					162:175	its high structural reconstruction	142:175	its high structural reconstruction	142:175	Nowadays 3D bioprinting, due to its high structural reconstruction and low cost, has been a promising technology and gained expectation in the treatment of vascular diseases.					
31870866	2	68	theme	3D	326:327	arg1	printing					329:336	3D printing	326:336	3D printing of large-sized blood vessels in the human body	326:383	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	4	69	theme	hollow	931:936	arg1	scaffolds					946:954	hollow tubular scaffolds	931:954	hollow tubular scaffolds	931:954	Mouse epidermal fibroblasts were inoculated into the inner and outer walls of hollow tubular scaffolds to fabricate engineered blood vessels.					
31870866	7	70	theme	carbon	1358:1363	arg1	nanotubes					1365:1373	carbon nanotubes	1358:1373	carbon nanotubes	1358:1373	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	3	71	theme	nozzle	802:807	arg1	extrusion					780:788	the vertical directional extrusion	755:788	the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module	755:850	In this paper, the hybrid bioink prepared with gelatin, sodium alginate and carbon nanotubes were manufactured into cylindrical scaffolds through the collaboration between the vertical directional extrusion of printing nozzle and axial rotation of stepper motor module.					
31870866	0	72	theme	3D	76:77	arg1	printing					79:86	direct 3D printing	69:86	direct 3D printing of vessel constructs	69:107	Engineering gelatin-based alginate/carbon nanotubes blend bioink for direct 3D printing of vessel constructs.					
31870866	5	73	theme	bionic	1025:1030	arg1	tubes					1041:1045	the bionic circular tubes	1021:1045	the bionic circular tubes printed in batches	1021:1064	The internal diameters of the bionic circular tubes printed in batches were 3 mm with an average wall thickness of 0.5 mm and a length of 7-10 cm.					
31870866	2	74	theme	vessels	359:365	arg1	printing					329:336	3D printing	326:336	3D printing of large-sized blood vessels in the human body	326:383	Although some studies have reported that 3D printing of large-sized blood vessels in the human body has been achieved, there are still some problems to be solved urgently, such as the unfulfilled microvascular simulation and inferior biocompatibility and mechanical strength of scaffold materials.					
31870866	7	75	contain	had	1375:1377	arg1	doping					1348:1353	doping	1348:1353	doping of carbon nanotubes	1348:1373	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	7	75	contain	had	1375:1377	arg2	effect					1386:1391	little effect	1379:1391	little effect	1379:1391	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
31870866	7	75	contain	had	1375:1377	arg1	amount					1338:1343	a small amount	1330:1343	a small amount of doping of carbon nanotubes	1330:1373	Also, quantitative experiments proved that a small amount of doping of carbon nanotubes had little effect on cytotoxicity, and the constructs could meet the requirements of biomimetic vascular.					
30376409	5	0	theme	encapsulation	1019:1031	arg1	process					1033:1039	the encapsulation process	1015:1039	the encapsulation process	1015:1039	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	6	1	from	bioavailability	1102:1116	arg1	CUR-CS-PEC-NPs					1149:1162	CUR-CS-PEC-NPs	1149:1162	CUR-CS-PEC-NPs	1149:1162	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs was enhanced significantly by 4-folds after 6 hours of treatment compared to free curcumin.					
30376409	8	2	theme	potential	1384:1392	arg1	application					1394:1404	the potential application	1380:1404	the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer	1380:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
30376409	6	3	from	curcumin	1137:1144	arg1	CUR-CS-PEC-NPs					1149:1162	CUR-CS-PEC-NPs	1149:1162	CUR-CS-PEC-NPs	1149:1162	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs was enhanced significantly by 4-folds after 6 hours of treatment compared to free curcumin.					
30376409	2	4	from	absorption	330:339	arg1	medium					417:422	acidic medium	410:422	acidic medium	410:422	However, its efficacy as an anticancer agent is restricted due to poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium.					
30376409	4	5	theme	cancer	785:790	arg1	cells					792:796	HT-29 colorectal cancer cells	768:796	HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation	768:876	The CUR-CS-PEC-NPs was taken up by HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation.					
30376409	8	6	from	application	1394:1404	arg1	delivery					1440:1447	the oral delivery	1431:1447	the oral delivery of curcumin in the treatment of colon cancer	1431:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
30376409	6	7	dep	bioavailability	1102:1116	arg1	AUC					1129:1131	AUC	1129:1131	AUC	1129:1131	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs was enhanced significantly by 4-folds after 6 hours of treatment compared to free curcumin.					
30376409	6	7	dep	bioavailability	1102:1116	arg1	Cmax					1119:1122	Cmax	1119:1122	Cmax	1119:1122	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs was enhanced significantly by 4-folds after 6 hours of treatment compared to free curcumin.					
30376409	4	8	theme	colorectal	774:783	arg1	cells					792:796	HT-29 colorectal cancer cells	768:796	HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation	768:876	The CUR-CS-PEC-NPs was taken up by HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation.					
30376409	8	9	from	delivery	1440:1447	arg1	treatment					1468:1476	the treatment	1464:1476	the treatment of colon cancer	1464:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
30376409	8	9	from	delivery	1440:1447	arg1	application					1394:1404	the potential application	1380:1404	the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer	1380:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
30376409	5	10	theme	free	961:964	arg1	curcumin					966:973	free curcumin	961:973	free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin	961:1090	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	7	11	theme	free	1341:1344	arg1	curcumin					1346:1353	free curcumin	1341:1353	free curcumin	1341:1353	Furthermore, the clearance of curcumin from the CUR-CS-PEC-NPs was lower compared to free curcumin.					
30376409	2	12	theme	anticancer	287:296	arg1	agent					298:302	an anticancer agent	284:302	an anticancer agent	284:302	However, its efficacy as an anticancer agent is restricted due to poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium.					
30376409	8	13	theme	CUR-CS-PEC-NPs	1413:1426	arg1	application					1394:1404	the potential application	1380:1404	the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer	1380:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
30376409	2	14	theme	acidic	410:415	arg1	medium					417:422	acidic medium	410:422	acidic medium	410:422	However, its efficacy as an anticancer agent is restricted due to poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium.					
30376409	4	15	theme	cell	861:864	arg1	propagation					866:876	cancer cell propagation	854:876	cancer cell propagation	854:876	The CUR-CS-PEC-NPs was taken up by HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation.					
30376409	2	16	from	tract	367:371	arg1	absorption					330:339	poor absorption	325:339	poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium	325:422	However, its efficacy as an anticancer agent is restricted due to poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium.					
30376409	2	17	theme	poor	325:328	arg1	absorption					330:339	poor absorption	325:339	poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium	325:422	However, its efficacy as an anticancer agent is restricted due to poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium.					
30376409	3	18	theme	present	432:438	arg1	study					440:444	the present study	428:444	the present study	428:444	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	0	19	theme	Pharmacokinetic	0:14	arg1	properties					38:47	Pharmacokinetic and anti-colon cancer properties	0:47	Pharmacokinetic and anti-colon cancer properties of curcumin-containing	0:70	Pharmacokinetic and anti-colon cancer properties of curcumin-containing chitosan-pectinate composite nanoparticles.					
30376409	2	20	from	metabolism	380:389	arg1	absorption					330:339	poor absorption	325:339	poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium	325:422	However, its efficacy as an anticancer agent is restricted due to poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium.					
30376409	1	21	theme	anticancer	212:221	arg1	Curcumin					116:123	Curcumin	116:123	Curcumin	116:123	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	1	21	theme	anticancer	212:221	arg1	agent					223:227	an anticancer agent	209:227	an anticancer agent for various types of tumours	209:256	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	0	22	theme	cancer	31:36	arg1	properties					38:47	Pharmacokinetic and anti-colon cancer properties	0:47	Pharmacokinetic and anti-colon cancer properties of curcumin-containing	0:70	Pharmacokinetic and anti-colon cancer properties of curcumin-containing chitosan-pectinate composite nanoparticles.					
30376409	7	23	from	CUR-CS-PEC-NPs	1304:1317	arg1	lower					1323:1327	lower	1323:1327	lower	1323:1327	Furthermore, the clearance of curcumin from the CUR-CS-PEC-NPs was lower compared to free curcumin.					
30376409	7	23	from	CUR-CS-PEC-NPs	1304:1317	arg1	clearance					1273:1281	the clearance	1269:1281	the clearance of curcumin from the CUR-CS-PEC-NPs	1269:1317	Furthermore, the clearance of curcumin from the CUR-CS-PEC-NPs was lower compared to free curcumin.					
30376409	8	24	theme	oral	1435:1438	arg1	delivery					1440:1447	the oral delivery	1431:1447	the oral delivery of curcumin in the treatment of colon cancer	1431:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
30376409	1	25	theme	active	130:135	arg1	Curcumin					116:123	Curcumin	116:123	Curcumin	116:123	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	1	25	theme	active	130:135	arg1	ingredient					137:146	the active ingredient	126:146	the active ingredient of the rhizome curcuma longa	126:175	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	0	26	theme	anti-colon	20:29	arg1	properties					38:47	Pharmacokinetic and anti-colon cancer properties	0:47	Pharmacokinetic and anti-colon cancer properties of curcumin-containing	0:70	Pharmacokinetic and anti-colon cancer properties of curcumin-containing chitosan-pectinate composite nanoparticles.					
30376409	3	27	theme	curcumin	552:559	arg1	deployment					538:547	deployment	538:547	deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival	538:730	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	5	28	theme	equivalent	978:987	arg1	doses					989:993	equivalent doses	978:993	equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin	978:1090	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	5	29	theme	anticancer	1060:1069	arg1	activity					1071:1078	the anticancer activity	1056:1078	the anticancer activity of curcumin	1056:1090	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	3	30	theme	chitosan-pectinate	475:492	arg1	CUR-CS-PEC-NPs					518:531	CUR-CS-PEC-NPs	518:531	CUR-CS-PEC-NPs	518:531	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	3	30	theme	chitosan-pectinate	475:492	arg1	system					510:515	chitosan-pectinate nanoparticulate system	475:515	chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs)	475:532	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	3	31	theme	degradative	613:623	arg1	effects					625:631	degradative effects	613:631	degradative effects in the upper digestive tract	613:660	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	3	32	from	effects	625:631	arg1	tract					656:660	the upper digestive tract	636:660	the upper digestive tract	636:660	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	1	33	theme	various	233:239	arg1	types					241:245	various types	233:245	various types of tumours	233:256	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	5	34	theme	anti-proliferative	883:900	arg1	effect					902:907	The anti-proliferative effect	879:907	The anti-proliferative effect of the encapsulated curcumin	879:936	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	5	34	theme	anti-proliferative	883:900	arg1	similar					942:948	similar	942:948	similar	942:948	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	6	35	theme	curcumin	1137:1144	arg1	bioavailability					1102:1116	The oral bioavailability	1093:1116	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs	1093:1162	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs was enhanced significantly by 4-folds after 6 hours of treatment compared to free curcumin.					
30376409	8	36	theme	cancer	1487:1492	arg1	treatment					1468:1476	the treatment	1464:1476	the treatment of colon cancer	1464:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
30376409	4	37	theme	significant	829:839	arg1	reduction					841:849	a significant reduction	827:849	a significant reduction in cancer cell propagation	827:876	The CUR-CS-PEC-NPs was taken up by HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation.					
30376409	2	38	from	degradation	395:405	arg1	absorption					330:339	poor absorption	325:339	poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium	325:422	However, its efficacy as an anticancer agent is restricted due to poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium.					
30376409	6	39	theme	oral	1097:1100	arg1	bioavailability					1102:1116	The oral bioavailability	1093:1116	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs	1093:1162	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs was enhanced significantly by 4-folds after 6 hours of treatment compared to free curcumin.					
30376409	5	40	theme	encapsulated	916:927	arg1	curcumin					929:936	the encapsulated curcumin	912:936	the encapsulated curcumin	912:936	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	8	41	theme	colon	1481:1485	arg1	cancer					1487:1492	colon cancer	1481:1492	colon cancer	1481:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
30376409	4	42	theme	cancer	854:859	arg1	propagation					866:876	cancer cell propagation	854:876	cancer cell propagation	854:876	The CUR-CS-PEC-NPs was taken up by HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation.					
30376409	3	43	from	curcumin	463:470	arg1	CUR-CS-PEC-NPs					518:531	CUR-CS-PEC-NPs	518:531	CUR-CS-PEC-NPs	518:531	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	3	43	from	curcumin	463:470	arg1	system					510:515	chitosan-pectinate nanoparticulate system	475:515	chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs)	475:532	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	2	44	theme	gastrointestinal	350:365	arg1	tract					367:371	the gastrointestinal tract	346:371	the gastrointestinal tract	346:371	However, its efficacy as an anticancer agent is restricted due to poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium.					
30376409	0	45	theme	curcumin-containing	52:70	arg1	properties					38:47	Pharmacokinetic and anti-colon cancer properties	0:47	Pharmacokinetic and anti-colon cancer properties of curcumin-containing	0:70	Pharmacokinetic and anti-colon cancer properties of curcumin-containing chitosan-pectinate composite nanoparticles.					
30376409	3	46	theme	nanoparticulate	494:508	arg1	CUR-CS-PEC-NPs					518:531	CUR-CS-PEC-NPs	518:531	CUR-CS-PEC-NPs	518:531	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	3	46	theme	nanoparticulate	494:508	arg1	system					510:515	chitosan-pectinate nanoparticulate system	475:515	chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs)	475:532	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	5	47	theme	curcumin	1083:1090	arg1	activity					1071:1078	the anticancer activity	1056:1078	the anticancer activity of curcumin	1056:1090	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	4	48	from	reduction	841:849	arg1	propagation					866:876	cancer cell propagation	854:876	cancer cell propagation	854:876	The CUR-CS-PEC-NPs was taken up by HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation.					
30376409	6	49	dep	treatment	1219:1227	arg1	6 hours					1208:1214	6 hours	1208:1214	6 hours	1208:1214	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs was enhanced significantly by 4-folds after 6 hours of treatment compared to free curcumin.					
30376409	8	50	theme	curcumin	1452:1459	arg1	delivery					1440:1447	the oral delivery	1431:1447	the oral delivery of curcumin in the treatment of colon cancer	1431:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
30376409	7	51	theme	curcumin	1286:1293	arg1	lower					1323:1327	lower	1323:1327	lower	1323:1327	Furthermore, the clearance of curcumin from the CUR-CS-PEC-NPs was lower compared to free curcumin.					
30376409	7	51	theme	curcumin	1286:1293	arg1	clearance					1273:1281	the clearance	1269:1281	the clearance of curcumin from the CUR-CS-PEC-NPs	1269:1317	Furthermore, the clearance of curcumin from the CUR-CS-PEC-NPs was lower compared to free curcumin.					
30376409	3	52	theme	anticancer	690:699	arg1	properties					701:710	its anticancer properties	686:710	its anticancer properties	686:710	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	3	53	theme	upper	640:644	arg1	tract					656:660	the upper digestive tract	636:660	the upper digestive tract	636:660	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	1	54	theme	rhizome	155:161	arg1	longa					171:175	the rhizome curcuma longa	151:175	the rhizome curcuma longa	151:175	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	4	55	theme	HT-29	768:772	arg1	cells					792:796	HT-29 colorectal cancer cells	768:796	HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation	768:876	The CUR-CS-PEC-NPs was taken up by HT-29 colorectal cancer cells which ultimately resulted in a significant reduction in cancer cell propagation.					
30376409	6	56	theme	free	1241:1244	arg1	curcumin					1246:1253	free curcumin	1241:1253	free curcumin	1241:1253	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs was enhanced significantly by 4-folds after 6 hours of treatment compared to free curcumin.					
30376409	3	57	theme	digestive	646:654	arg1	tract					656:660	the upper digestive tract	636:660	the upper digestive tract	636:660	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	1	58	theme	curcuma	163:169	arg1	longa					171:175	the rhizome curcuma longa	151:175	the rhizome curcuma longa	151:175	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	1	59	theme	tumours	250:256	arg1	types					241:245	various types	233:245	various types of tumours	233:256	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	6	60	from	CUR-CS-PEC-NPs	1149:1162	arg1	bioavailability					1102:1116	The oral bioavailability	1093:1116	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs	1093:1162	The oral bioavailability (Cmax, and AUC) of curcumin in CUR-CS-PEC-NPs was enhanced significantly by 4-folds after 6 hours of treatment compared to free curcumin.					
30376409	5	61	theme	curcumin	929:936	arg1	effect					902:907	The anti-proliferative effect	879:907	The anti-proliferative effect of the encapsulated curcumin	879:936	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	5	61	theme	curcumin	929:936	arg1	similar					942:948	similar	942:948	similar	942:948	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	1	62	theme	longa	171:175	arg1	Curcumin					116:123	Curcumin	116:123	Curcumin	116:123	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	1	62	theme	longa	171:175	arg1	ingredient					137:146	the active ingredient	126:146	the active ingredient of the rhizome curcuma longa	126:175	Curcumin, the active ingredient of the rhizome curcuma longa has been extensively studied as an anticancer agent for various types of tumours.					
30376409	5	63	from	doses	989:993	arg1	curcumin					966:973	free curcumin	961:973	free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin	961:1090	The anti-proliferative effect of the encapsulated curcumin was similar to that of free curcumin at equivalent doses which confirms that the encapsulation process did not impede the anticancer activity of curcumin.					
30376409	0	64	theme	composite	91:99	arg1	nanoparticles					101:113	composite nanoparticles	91:113	composite nanoparticles	91:113	Pharmacokinetic and anti-colon cancer properties of curcumin-containing chitosan-pectinate composite nanoparticles.					
30376409	3	65	theme	colon	718:722	arg1	arrival					724:730	colon arrival	718:730	colon arrival	718:730	In the present study, we encapsulated curcumin in chitosan-pectinate nanoparticulate system (CUR-CS-PEC-NPs) for deployment of curcumin to the colon, whereby curcumin is protected against degradative effects in the upper digestive tract, and hence, maintaining its anticancer properties until colon arrival.					
30376409	2	66	theme	rapid	374:378	arg1	metabolism					380:389	rapid metabolism	374:389	rapid metabolism	374:389	However, its efficacy as an anticancer agent is restricted due to poor absorption from the gastrointestinal tract, rapid metabolism and degradation in acidic medium.					
30376409	8	67	from	CUR-CS-PEC-NPs	1413:1426	arg1	delivery					1440:1447	the oral delivery	1431:1447	the oral delivery of curcumin in the treatment of colon cancer	1431:1492	These findings point to the potential application of the CUR-CS-PEC-NPs in the oral delivery of curcumin in the treatment of colon cancer.					
31830628	4	0	dep	showed	969:974	arg1	proved					992:997	proved	992:997	proved by the 5.5% reduction in photocatalytic capability after five cyclic tests	992:1072	BC/PDA/TiO2 also showed good stability, proved by the 5.5% reduction in photocatalytic capability after five cyclic tests.					
31830628	3	1	theme	BC/PDA/TiO2	753:763	arg1	composite					765:773	the as-prepared BC/PDA/TiO2 composite	737:773	the as-prepared BC/PDA/TiO2 composite	737:773	Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved.					
31830628	0	2	with	membrane	70:77	arg1	adsorption					93:102	enhanced adsorption	84:102	enhanced adsorption	84:102	Three-dimensional bacterial cellulose/polydopamine/TiO2 nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under ultraviolet-visible irradiation.					
31830628	1	3	theme	efficient	224:232	arg1	property					234:241	efficient property	224:241	efficient property	224:241	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31830628	5	4	theme	photo-degradation	1153:1169	arg1	performance					1171:1181	excellent photo-degradation performance	1143:1181	excellent photo-degradation performance	1143:1181	We expect our design could provide a facile and green approach with excellent photo-degradation performance for organic pollutants, providing further applications for photocatalysis and wastewater treatment.					
31830628	1	5	theme	pollutants	320:329	arg1	elimination					297:307	the elimination	293:307	the elimination of organic pollutants in wastewater	293:343	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31830628	2	6	theme	soft	552:555	arg1	template					557:564	a three-dimensional soft template	532:564	a three-dimensional soft template	532:564	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	2	6	theme	soft	552:555	arg1	nanofibers					518:527	BC nanofibers	515:527	BC nanofibers	515:527	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	4	7	from	reduction	1011:1019	arg1	capability					1039:1048	photocatalytic capability	1024:1048	photocatalytic capability	1024:1048	BC/PDA/TiO2 also showed good stability, proved by the 5.5% reduction in photocatalytic capability after five cyclic tests.					
31830628	4	8	theme	%	1009:1009	arg1	reduction					1011:1019	the 5.5% reduction	1002:1019	the 5.5% reduction in photocatalytic capability after five cyclic tests	1002:1072	BC/PDA/TiO2 also showed good stability, proved by the 5.5% reduction in photocatalytic capability after five cyclic tests.					
31830628	5	9	theme	further	1217:1223	arg1	applications					1225:1236	further applications	1217:1236	further applications	1217:1236	We expect our design could provide a facile and green approach with excellent photo-degradation performance for organic pollutants, providing further applications for photocatalysis and wastewater treatment.					
31830628	0	10	with	degradation	123:133	arg1	adsorption					93:102	enhanced adsorption	84:102	enhanced adsorption	84:102	Three-dimensional bacterial cellulose/polydopamine/TiO2 nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under ultraviolet-visible irradiation.					
31830628	3	11	theme	higher	785:790	arg1	capacity					803:810	higher adsorption capacity	785:810	higher adsorption capacity for methyl orange, Rhodamine B and methylene blue	785:860	Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved.					
31830628	2	12	theme	protective	629:638	arg1	agent					640:644	a protective agent	627:644	a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles	627:704	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	3	13	theme	photocatalytic	871:884	arg1	properties					886:895	the photocatalytic properties	867:895	the photocatalytic properties within 30 min after irradiation	867:927	Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved.					
31830628	3	14	theme	adsorption	792:801	arg1	capacity					803:810	higher adsorption capacity	785:810	higher adsorption capacity for methyl orange, Rhodamine B and methylene blue	785:860	Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved.					
31830628	1	15	with	production	191:200	arg1	reusability					254:264	stable reusability	247:264	stable reusability	247:264	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31830628	1	15	with	production	191:200	arg1	property					234:241	efficient property	224:241	efficient property	224:241	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31830628	2	16	theme	UV	492:493	arg1	radiation					495:503	UV radiation	492:503	UV radiation	492:503	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	0	17	theme	ultraviolet-visible	150:168	arg1	irradiation					170:180	ultraviolet-visible irradiation	150:180	ultraviolet-visible irradiation	150:180	Three-dimensional bacterial cellulose/polydopamine/TiO2 nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under ultraviolet-visible irradiation.					
31830628	2	18	theme	dioxide	677:683	arg1	nanoparticles					692:704	titanium dioxide (TiO2) nanoparticles	668:704	titanium dioxide (TiO2) nanoparticles	668:704	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	1	19	theme	stable	247:252	arg1	reusability					254:264	stable reusability	247:264	stable reusability	247:264	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31830628	0	20	theme	bacterial	18:26	arg1	cellulose/polydopamine/TiO2					28:54	Three-dimensional bacterial cellulose/polydopamine/TiO2	0:54	Three-dimensional bacterial cellulose/polydopamine/TiO2	0:54	Three-dimensional bacterial cellulose/polydopamine/TiO2 nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under ultraviolet-visible irradiation.					
31830628	4	21	theme	good	976:979	arg1	stability					981:989	good stability	976:989	good stability	976:989	BC/PDA/TiO2 also showed good stability, proved by the 5.5% reduction in photocatalytic capability after five cyclic tests.					
31830628	2	22	theme	titanium	668:675	arg1	TiO2					686:689	TiO2	686:689	TiO2	686:689	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	2	22	theme	titanium	668:675	arg1	dioxide					677:683	titanium dioxide	668:683	titanium dioxide (TiO2) nanoparticles	668:704	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	5	23	theme	wastewater	1261:1270	arg1	treatment					1272:1280	wastewater treatment	1261:1280	wastewater treatment	1261:1280	We expect our design could provide a facile and green approach with excellent photo-degradation performance for organic pollutants, providing further applications for photocatalysis and wastewater treatment.					
31830628	2	24	theme	photocatalytic	450:463	arg1	degradation					465:475	photocatalytic degradation	450:475	photocatalytic degradation	450:475	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	0	25	theme	Three-dimensional	0:16	arg1	cellulose/polydopamine/TiO2					28:54	Three-dimensional bacterial cellulose/polydopamine/TiO2	0:54	Three-dimensional bacterial cellulose/polydopamine/TiO2	0:54	Three-dimensional bacterial cellulose/polydopamine/TiO2 nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under ultraviolet-visible irradiation.					
31830628	3	26	theme	methyl	816:821	arg1	orange					823:828	methyl orange	816:828	methyl orange	816:828	Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved.					
31830628	2	27	theme	cellulose	377:385	arg1	membrane					412:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	2	28	theme	enhanced	426:433	arg1	adsorption					435:444	enhanced adsorption	426:444	enhanced adsorption	426:444	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	2	29	theme	functional	606:615	arg1	layer					617:621	a functional layer	604:621	a functional layer	604:621	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	2	30	theme	bacterial	367:375	arg1	membrane					412:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	5	31	theme	green	1123:1127	arg1	approach					1129:1136	a facile and green approach	1110:1136	a facile and green approach with excellent photo-degradation performance for organic pollutants	1110:1204	We expect our design could provide a facile and green approach with excellent photo-degradation performance for organic pollutants, providing further applications for photocatalysis and wastewater treatment.					
31830628	1	32	theme	great	272:276	arg1	importance					278:287	great importance	272:287	great importance	272:287	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31830628	2	33	theme	three-dimensional	534:550	arg1	template					557:564	a three-dimensional soft template	532:564	a three-dimensional soft template	532:564	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	2	33	theme	three-dimensional	534:550	arg1	nanofibers					518:527	BC nanofibers	515:527	BC nanofibers	515:527	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	2	34	theme	-based	391:396	arg1	membrane					412:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	5	35	with	approach	1129:1136	arg1	performance					1171:1181	excellent photo-degradation performance	1143:1181	excellent photo-degradation performance	1143:1181	We expect our design could provide a facile and green approach with excellent photo-degradation performance for organic pollutants, providing further applications for photocatalysis and wastewater treatment.					
31830628	4	36	theme	cyclic	1061:1066	arg1	tests					1068:1072	five cyclic tests	1056:1072	five cyclic tests	1056:1072	BC/PDA/TiO2 also showed good stability, proved by the 5.5% reduction in photocatalytic capability after five cyclic tests.					
31830628	5	37	theme	excellent	1143:1151	arg1	performance					1171:1181	excellent photo-degradation performance	1143:1181	excellent photo-degradation performance	1143:1181	We expect our design could provide a facile and green approach with excellent photo-degradation performance for organic pollutants, providing further applications for photocatalysis and wastewater treatment.					
31830628	2	38	theme	nanocomposite	398:410	arg1	membrane					412:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	0	39	theme	enhanced	84:91	arg1	adsorption					93:102	enhanced adsorption	84:102	enhanced adsorption	84:102	Three-dimensional bacterial cellulose/polydopamine/TiO2 nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under ultraviolet-visible irradiation.					
31830628	5	40	theme	organic	1187:1193	arg1	pollutants					1195:1204	organic pollutants	1187:1204	organic pollutants	1187:1204	We expect our design could provide a facile and green approach with excellent photo-degradation performance for organic pollutants, providing further applications for photocatalysis and wastewater treatment.					
31830628	3	41	theme	commercial	721:730	arg1	P25					732:734	commercial P25	721:734	commercial P25	721:734	Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved.					
31830628	1	42	theme	Cleaner	183:189	arg1	production					191:200	Cleaner production	183:200	Cleaner production of photocatalyst with efficient property and stable reusability	183:264	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31830628	3	43	theme	Rhodamine	831:839	arg1	B					841:841	Rhodamine B	831:841	Rhodamine B	831:841	Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved.					
31830628	2	44	theme	nanoparticles	692:704	arg1	immobilization					650:663	immobilization	650:663	immobilization of titanium dioxide (TiO2) nanoparticles	650:704	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	1	45	from	elimination	297:307	arg1	wastewater					334:343	wastewater	334:343	wastewater	334:343	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31830628	5	46	theme	facile	1112:1117	arg1	approach					1129:1136	a facile and green approach	1110:1136	a facile and green approach with excellent photo-degradation performance for organic pollutants	1110:1204	We expect our design could provide a facile and green approach with excellent photo-degradation performance for organic pollutants, providing further applications for photocatalysis and wastewater treatment.					
31830628	2	47	theme	BC	388:389	arg1	membrane					412:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	a bacterial cellulose (BC)-based nanocomposite membrane	365:419	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	0	48	theme	photocatalytic	108:121	arg1	degradation					123:133	photocatalytic degradation	108:133	photocatalytic degradation	108:133	Three-dimensional bacterial cellulose/polydopamine/TiO2 nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under ultraviolet-visible irradiation.					
31830628	1	49	theme	photocatalyst	205:217	arg1	production					191:200	Cleaner production	183:200	Cleaner production of photocatalyst with efficient property and stable reusability	183:264	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31830628	4	50	theme	photocatalytic	1024:1037	arg1	capability					1039:1048	photocatalytic capability	1024:1048	photocatalytic capability	1024:1048	BC/PDA/TiO2 also showed good stability, proved by the 5.5% reduction in photocatalytic capability after five cyclic tests.					
31830628	3	51	theme	as-prepared	741:751	arg1	composite					765:773	the as-prepared BC/PDA/TiO2 composite	737:773	the as-prepared BC/PDA/TiO2 composite	737:773	Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved.					
31830628	3	52	theme	blue	857:860	arg1	methylene					847:855	methylene blue	847:860	methylene blue	847:860	Compared with commercial P25, the as-prepared BC/PDA/TiO2 composite presented higher adsorption capacity for methyl orange, Rhodamine B and methylene blue, and the photocatalytic properties within 30 min after irradiation were further improved.					
31830628	2	53	theme	BC	515:516	arg1	template					557:564	a three-dimensional soft template	532:564	a three-dimensional soft template	532:564	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	2	53	theme	BC	515:516	arg1	nanofibers					518:527	BC nanofibers	515:527	BC nanofibers	515:527	Herein, we present a bacterial cellulose (BC)-based nanocomposite membrane with enhanced adsorption and photocatalytic degradation for dyes under UV radiation, by using BC nanofibers as a three-dimensional soft template, coated with the polydoamine (PDA) as a functional layer and a protective agent for immobilization of titanium dioxide (TiO2) nanoparticles.					
31830628	1	54	theme	organic	312:318	arg1	pollutants					320:329	organic pollutants	312:329	organic pollutants	312:329	Cleaner production of photocatalyst with efficient property and stable reusability is of great importance for the elimination of organic pollutants in wastewater.					
31071402	7	0	theme	release	797:803	arg1	behavior					805:812	The release behavior	793:812	The release behavior at pH 9.0	793:822	The release behavior at pH 9.0 displayed a higher release rate than that at pH 7.0.					
31071402	5	1	theme	emulsion	639:646	arg1	beads					667:671	emulsion filled SA hydrogel beads	639:671	emulsion filled SA hydrogel beads	639:671	For Cur, the EE and LC of emulsion filled SA hydrogel beads were 99.15 ± 0.85% and 7.25 ± 3.16 mg/g respectively.					
31071402	9	2	theme	electron	1085:1092	arg1	FE-SEM					1106:1111	FE-SEM	1106:1111	FE-SEM	1106:1111	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	9	2	theme	electron	1085:1092	arg1	microscope					1094:1103	field emission scanning electron microscope	1061:1103	field emission scanning electron microscope (FE-SEM)	1061:1112	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	0	3	theme	hydrogel	86:93	arg1	beads					95:99	nanoemulsions-filled alginate hydrogel beads	56:99	nanoemulsions-filled alginate hydrogel beads	56:99	Encapsulation and release behavior of curcumin based on nanoemulsions-filled alginate hydrogel beads.					
31071402	10	4	contain	have	1331:1334	arg1	beads					1320:1324	hybrid hydrogel beads	1304:1324	hybrid hydrogel beads that have potential in delivery system for hydrophobic composition	1304:1391	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	10	4	contain	have	1331:1334	arg2	potential					1336:1344	potential	1336:1344	potential	1336:1344	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	5	5	theme	filled	648:653	arg1	beads					667:671	emulsion filled SA hydrogel beads	639:671	emulsion filled SA hydrogel beads	639:671	For Cur, the EE and LC of emulsion filled SA hydrogel beads were 99.15 ± 0.85% and 7.25 ± 3.16 mg/g respectively.					
31071402	10	6	theme	hybrid	1304:1309	arg1	beads					1320:1324	hybrid hydrogel beads	1304:1324	hybrid hydrogel beads that have potential in delivery system for hydrophobic composition	1304:1391	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	5	7	theme	SA	655:656	arg1	beads					667:671	emulsion filled SA hydrogel beads	639:671	emulsion filled SA hydrogel beads	639:671	For Cur, the EE and LC of emulsion filled SA hydrogel beads were 99.15 ± 0.85% and 7.25 ± 3.16 mg/g respectively.					
31071402	10	8	theme	combined	1240:1247	arg1	technique					1249:1257	a practical combined technique	1228:1257	a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition	1228:1391	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	5	9	theme	hydrogel	658:665	arg1	beads					667:671	emulsion filled SA hydrogel beads	639:671	emulsion filled SA hydrogel beads	639:671	For Cur, the EE and LC of emulsion filled SA hydrogel beads were 99.15 ± 0.85% and 7.25 ± 3.16 mg/g respectively.					
31071402	1	10	theme	self-emulsification	274:292	arg1	method					264:269	the method	260:269	the method of self-emulsification and sodium alginate (SA) ionic gelation	260:332	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	8	11	theme	diffusion-controlled	981:1000	arg1	model					1002:1006	a diffusion-controlled model	979:1006	a diffusion-controlled model	979:1006	Cur released behavior well followed the Hixcon-Crowell model which indicated that Cur was released in a diffusion-controlled model.					
31071402	4	12	with	shell	570:574	arg1	diameter					585:592	the diameter	581:592	the diameter of 0.46 ± 0.02 mm	581:610	The nanoemulsions filled SA hydrogel beads were spherical shell with the diameter of 0.46 ± 0.02 mm.					
31071402	1	13	theme	facile	168:173	arg1	approach					175:182	a facile approach	166:182	a facile approach	166:182	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	4	14	theme	0.46 ± 0.02 mm	597:610	arg1	diameter					585:592	the diameter	581:592	the diameter of 0.46 ± 0.02 mm	581:610	The nanoemulsions filled SA hydrogel beads were spherical shell with the diameter of 0.46 ± 0.02 mm.					
31071402	8	15	theme	released	881:888	arg1	behavior					890:897	Cur released behavior	877:897	Cur released behavior	877:897	Cur released behavior well followed the Hixcon-Crowell model which indicated that Cur was released in a diffusion-controlled model.					
31071402	3	16	theme	Cur	446:448	arg1	nanoemulsions					459:471	Cur packaged nanoemulsions	446:471	Cur packaged nanoemulsions	446:471	The results indicated that Cur packaged nanoemulsions were with the size of 24.26 ± 0.22 nm.					
31071402	8	17	theme	Cur	877:879	arg1	behavior					890:897	Cur released behavior	877:897	Cur released behavior	877:897	Cur released behavior well followed the Hixcon-Crowell model which indicated that Cur was released in a diffusion-controlled model.					
31071402	0	18	theme	Encapsulation	0:12	arg1	behavior					26:33	Encapsulation and release behavior	0:33	Encapsulation and release behavior of curcumin	0:45	Encapsulation and release behavior of curcumin based on nanoemulsions-filled alginate hydrogel beads.					
31071402	7	19	from	pH 9.0	817:822	arg1	behavior					805:812	The release behavior	793:812	The release behavior at pH 9.0	793:822	The release behavior at pH 9.0 displayed a higher release rate than that at pH 7.0.					
31071402	7	20	theme	higher	836:841	arg1	rate					851:854	a higher release rate	834:854	a higher release rate	834:854	The release behavior at pH 9.0 displayed a higher release rate than that at pH 7.0.					
31071402	1	21	theme	sodium	298:303	arg1	gelation					325:332	sodium alginate (SA) ionic gelation	298:332	sodium alginate (SA) ionic gelation	298:332	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	6	22	theme	pH	779:780	arg1	condition					782:790	external pH condition	770:790	external pH condition	770:790	The release behavior could be regulated by external pH condition.					
31071402	7	23	theme	release	843:849	arg1	rate					851:854	a higher release rate	834:854	a higher release rate	834:854	The release behavior at pH 9.0 displayed a higher release rate than that at pH 7.0.					
31071402	0	24	theme	release	18:24	arg1	behavior					26:33	Encapsulation and release behavior	0:33	Encapsulation and release behavior of curcumin	0:45	Encapsulation and release behavior of curcumin based on nanoemulsions-filled alginate hydrogel beads.					
31071402	1	25	theme	alginate	305:312	arg1	gelation					325:332	sodium alginate (SA) ionic gelation	298:332	sodium alginate (SA) ionic gelation	298:332	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	9	26	theme	beads	1168:1172	arg1	behavior					1149:1156	the corrosion behavior	1135:1156	the corrosion behavior of SA gel beads during Cur release	1135:1191	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	2	27	dep	encapsulation	339:351	arg1	behavior					365:372	behavior	365:372	behavior	365:372	The encapsulation and release behavior of curcumin (Cur) were further investigated.					
31071402	2	27	dep	encapsulation	339:351	arg1	The					335:337	The	335:337	The	335:337	The encapsulation and release behavior of curcumin (Cur) were further investigated.					
31071402	10	28	theme	delivery	1349:1356	arg1	system					1358:1363	delivery system	1349:1363	delivery system for hydrophobic composition	1349:1391	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	0	29	theme	curcumin	38:45	arg1	behavior					26:33	Encapsulation and release behavior	0:33	Encapsulation and release behavior of curcumin	0:45	Encapsulation and release behavior of curcumin based on nanoemulsions-filled alginate hydrogel beads.					
31071402	10	30	theme	emulsions	1262:1270	arg1	technique					1249:1257	a practical combined technique	1228:1257	a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition	1228:1391	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	3	31	theme	packaged	450:457	arg1	nanoemulsions					459:471	Cur packaged nanoemulsions	446:471	Cur packaged nanoemulsions	446:471	The results indicated that Cur packaged nanoemulsions were with the size of 24.26 ± 0.22 nm.					
31071402	10	32	theme	gelation	1282:1289	arg1	technique					1249:1257	a practical combined technique	1228:1257	a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition	1228:1391	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	1	33	theme	SA	315:316	arg1	gelation					325:332	sodium alginate (SA) ionic gelation	298:332	sodium alginate (SA) ionic gelation	298:332	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	9	34	theme	SA	1161:1162	arg1	beads					1168:1172	SA gel beads	1161:1172	SA gel beads	1161:1172	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	6	35	theme	external	770:777	arg1	condition					782:790	external pH condition	770:790	external pH condition	770:790	The release behavior could be regulated by external pH condition.					
31071402	5	36	dep	EE	626:627	arg1	the					622:624	the	622:624	the	622:624	For Cur, the EE and LC of emulsion filled SA hydrogel beads were 99.15 ± 0.85% and 7.25 ± 3.16 mg/g respectively.					
31071402	4	37	theme	SA	537:538	arg1	beads					549:553	SA hydrogel beads	537:553	SA hydrogel beads	537:553	The nanoemulsions filled SA hydrogel beads were spherical shell with the diameter of 0.46 ± 0.02 mm.					
31071402	5	38	theme	beads	667:671	arg1	LC					633:634	LC	633:634	LC	633:634	For Cur, the EE and LC of emulsion filled SA hydrogel beads were 99.15 ± 0.85% and 7.25 ± 3.16 mg/g respectively.					
31071402	5	38	theme	beads	667:671	arg1	EE					626:627	EE	626:627	EE	626:627	For Cur, the EE and LC of emulsion filled SA hydrogel beads were 99.15 ± 0.85% and 7.25 ± 3.16 mg/g respectively.					
31071402	4	39	theme	spherical	560:568	arg1	shell					570:574	spherical shell	560:574	spherical shell	560:574	The nanoemulsions filled SA hydrogel beads were spherical shell with the diameter of 0.46 ± 0.02 mm.					
31071402	4	39	theme	spherical	560:568	arg1	nanoemulsions					516:528	The nanoemulsions	512:528	The nanoemulsions filled SA hydrogel beads	512:553	The nanoemulsions filled SA hydrogel beads were spherical shell with the diameter of 0.46 ± 0.02 mm.					
31071402	4	40	theme	hydrogel	540:547	arg1	beads					549:553	SA hydrogel beads	537:553	SA hydrogel beads	537:553	The nanoemulsions filled SA hydrogel beads were spherical shell with the diameter of 0.46 ± 0.02 mm.					
31071402	8	41	theme	Hixcon-Crowell	917:930	arg1	model					932:936	the Hixcon-Crowell model	913:936	the Hixcon-Crowell model which indicated that Cur was released in a diffusion-controlled model	913:1006	Cur released behavior well followed the Hixcon-Crowell model which indicated that Cur was released in a diffusion-controlled model.					
31071402	1	42	theme	bilateral	117:125	arg1	advantages					127:136	the bilateral advantages	113:136	the bilateral advantages of emulsions and hydrogels	113:163	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	1	43	theme	ionic	319:323	arg1	gelation					325:332	sodium alginate (SA) ionic gelation	298:332	sodium alginate (SA) ionic gelation	298:332	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	9	44	theme	corrosion	1139:1147	arg1	behavior					1149:1156	the corrosion behavior	1135:1156	the corrosion behavior of SA gel beads during Cur release	1135:1191	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	3	45	theme	24.26 ± 0.22 nm	495:509	arg1	size					487:490	the size	483:490	the size of 24.26 ± 0.22 nm	483:509	The results indicated that Cur packaged nanoemulsions were with the size of 24.26 ± 0.22 nm.					
31071402	9	46	theme	microstructure	1040:1053	arg1	investigation					1023:1035	investigation	1023:1035	investigation of microstructure using field emission scanning electron microscope (FE-SEM)	1023:1112	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	1	47	theme	gelation	325:332	arg1	method					264:269	the method	260:269	the method of self-emulsification and sodium alginate (SA) ionic gelation	260:332	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	1	48	used	used	188:191	arg2	approach					175:182	a facile approach	166:182	a facile approach	166:182	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	10	49	theme	ionic	1276:1280	arg1	gelation					1282:1289	ionic gelation	1276:1289	ionic gelation	1276:1289	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	10	50	theme	practical	1230:1238	arg1	technique					1249:1257	a practical combined technique	1228:1257	a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition	1228:1391	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	9	51	theme	gel	1164:1166	arg1	beads					1168:1172	SA gel beads	1161:1172	SA gel beads	1161:1172	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	10	52	theme	hydrophobic	1369:1379	arg1	composition					1381:1391	hydrophobic composition	1369:1391	hydrophobic composition	1369:1391	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	1	53	dep	filled	220:225	arg1	hydrogel					227:234	hydrogel	227:234	hydrogel	227:234	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	1	53	dep	filled	220:225	arg1	nanoemulsions					206:218	nanoemulsions	206:218	nanoemulsions filled hydrogel beads	206:240	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	1	53	dep	filled	220:225	arg1	beads					236:240	beads	236:240	beads	236:240	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	9	54	theme	Cur	1181:1183	arg1	release					1185:1191	Cur release	1181:1191	Cur release	1181:1191	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	1	55	theme	emulsions	141:149	arg1	advantages					127:136	the bilateral advantages	113:136	the bilateral advantages of emulsions and hydrogels	113:163	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	9	56	theme	field	1061:1065	arg1	FE-SEM					1106:1111	FE-SEM	1106:1111	FE-SEM	1106:1111	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	9	56	theme	field	1061:1065	arg1	microscope					1094:1103	field emission scanning electron microscope	1061:1103	field emission scanning electron microscope (FE-SEM)	1061:1112	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	0	57	theme	alginate	77:84	arg1	beads					95:99	nanoemulsions-filled alginate hydrogel beads	56:99	nanoemulsions-filled alginate hydrogel beads	56:99	Encapsulation and release behavior of curcumin based on nanoemulsions-filled alginate hydrogel beads.					
31071402	10	58	theme	hydrogel	1311:1318	arg1	beads					1320:1324	hybrid hydrogel beads	1304:1324	hybrid hydrogel beads that have potential in delivery system for hydrophobic composition	1304:1391	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
31071402	2	59	theme	curcumin	377:384	arg1	encapsulation					339:351	encapsulation	339:351	encapsulation	339:351	The encapsulation and release behavior of curcumin (Cur) were further investigated.					
31071402	2	59	theme	curcumin	377:384	arg1	release					357:363	release	357:363	release	357:363	The encapsulation and release behavior of curcumin (Cur) were further investigated.					
31071402	9	60	theme	emission	1067:1074	arg1	FE-SEM					1106:1111	FE-SEM	1106:1111	FE-SEM	1106:1111	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	9	60	theme	emission	1067:1074	arg1	microscope					1094:1103	field emission scanning electron microscope	1061:1103	field emission scanning electron microscope (FE-SEM)	1061:1112	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	0	61	theme	nanoemulsions-filled	56:75	arg1	beads					95:99	nanoemulsions-filled alginate hydrogel beads	56:99	nanoemulsions-filled alginate hydrogel beads	56:99	Encapsulation and release behavior of curcumin based on nanoemulsions-filled alginate hydrogel beads.					
31071402	6	62	theme	release	731:737	arg1	behavior					739:746	The release behavior	727:746	The release behavior	727:746	The release behavior could be regulated by external pH condition.					
31071402	1	63	theme	hydrogels	155:163	arg1	advantages					127:136	the bilateral advantages	113:136	the bilateral advantages of emulsions and hydrogels	113:163	To provide the bilateral advantages of emulsions and hydrogels, a facile approach was used to fabricate nanoemulsions filled hydrogel beads through combining the method of self-emulsification and sodium alginate (SA) ionic gelation.					
31071402	9	64	theme	scanning	1076:1083	arg1	FE-SEM					1106:1111	FE-SEM	1106:1111	FE-SEM	1106:1111	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	9	64	theme	scanning	1076:1083	arg1	microscope					1094:1103	field emission scanning electron microscope	1061:1103	field emission scanning electron microscope (FE-SEM)	1061:1112	Comparatively investigation of microstructure using field emission scanning electron microscope (FE-SEM) further investigates the corrosion behavior of SA gel beads during Cur release.					
31071402	10	65	theme	worth-while	1198:1208	arg1	endeavor					1210:1217	The worth-while endeavor	1194:1217	The worth-while endeavor	1194:1217	The worth-while endeavor provided a practical combined technique of emulsions and ionic gelation to fabricate hybrid hydrogel beads that have potential in delivery system for hydrophobic composition.					
30594836	0	0	theme	different	74:82	arg1	elements					90:97	different screw elements	74:97	different screw elements in a twin-screw extruder	74:122	Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder.					
30594836	5	1	theme	Sugar	687:691	arg1	yield					693:697	Sugar yield	687:697	Sugar yield	687:697	Sugar yield, crystallinity index, and the particle size distribution of the material collected at different zones were determined.					
30594836	4	2	theme	residuals	601:609	arg1	samples					611:617	the residuals samples	597:617	the residuals samples	597:617	To examine the influence of shear imparted by the screws, the residuals samples were recovered from different zones along the screws and evaluated.					
30594836	6	3	theme	Glucose	818:824	arg1	yield					826:830	Glucose yield	818:830	Glucose yield	818:830	Glucose yield and xylose/mannose yields of the material along the screws, ranged from 23.25 to 42.88% and from 11.95 to 20.54%, respectively.					
30594836	7	4	theme	design	988:993	arg1	importance					964:973	The importance	960:973	The importance of the screw design	960:993	The importance of the screw design was highlighted.					
30594836	0	5	theme	screw	84:88	arg1	elements					90:97	different screw elements	74:97	different screw elements in a twin-screw extruder	74:122	Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder.					
30594836	3	6	theme	Lower	467:471	arg1	temperatures					473:484	Lower temperatures	467:484	Lower temperatures	467:484	Lower temperatures and screw speeds resulted in increased sugar yields.					
30594836	1	7	theme	cellulose	254:262	arg1	conversion					236:245	the downstream enzymatic conversion	211:245	the downstream enzymatic conversion of the cellulose into fermentable sugars	211:286	Proper screw design is crucial for effectively pre-treating wood fibers, to assist in the downstream enzymatic conversion of the cellulose into fermentable sugars.					
30594836	2	8	theme	optimum	431:437	arg1	conditions					439:448	the optimum conditions	427:448	the optimum conditions for sugar yield	427:464	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	6	9	dep	42.88	913:917	arg1	to					910:911	to	910:911	to	910:911	Glucose yield and xylose/mannose yields of the material along the screws, ranged from 23.25 to 42.88% and from 11.95 to 20.54%, respectively.					
30594836	5	10	theme	material	763:770	arg1	yield					693:697	Sugar yield	687:697	Sugar yield	687:697	Sugar yield, crystallinity index, and the particle size distribution of the material collected at different zones were determined.					
30594836	5	10	theme	material	763:770	arg1	index					714:718	crystallinity index	700:718	crystallinity index	700:718	Sugar yield, crystallinity index, and the particle size distribution of the material collected at different zones were determined.					
30594836	5	10	theme	material	763:770	arg1	distribution					743:754	the particle size distribution	725:754	the particle size distribution of the material collected at different zones	725:799	Sugar yield, crystallinity index, and the particle size distribution of the material collected at different zones were determined.					
30594836	7	11	theme	screw	982:986	arg1	design					988:993	the screw design	978:993	the screw design	978:993	The importance of the screw design was highlighted.					
30594836	6	12	dep	20.54	938:942	arg1	to					935:936	to	935:936	to	935:936	Glucose yield and xylose/mannose yields of the material along the screws, ranged from 23.25 to 42.88% and from 11.95 to 20.54%, respectively.					
30594836	3	13	theme	screw	490:494	arg1	speeds					496:501	screw speeds	490:501	screw speeds	490:501	Lower temperatures and screw speeds resulted in increased sugar yields.					
30594836	1	14	theme	pre-treating	172:183	arg1	fibers					190:195	effectively pre-treating wood fibers	160:195	effectively pre-treating wood fibers	160:195	Proper screw design is crucial for effectively pre-treating wood fibers, to assist in the downstream enzymatic conversion of the cellulose into fermentable sugars.					
30594836	1	15	theme	wood	185:188	arg1	fibers					190:195	effectively pre-treating wood fibers	160:195	effectively pre-treating wood fibers	160:195	Proper screw design is crucial for effectively pre-treating wood fibers, to assist in the downstream enzymatic conversion of the cellulose into fermentable sugars.					
30594836	0	16	theme	biomass	30:36	arg1	Disruption					0:9	Disruption	0:9	Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder.	0:123	Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder.					
30594836	1	17	theme	fermentable	269:279	arg1	sugars					281:286	fermentable sugars	269:286	fermentable sugars	269:286	Proper screw design is crucial for effectively pre-treating wood fibers, to assist in the downstream enzymatic conversion of the cellulose into fermentable sugars.					
30594836	2	18	dep	temperature	330:340	arg1	150 °C					356:361	150 °C	356:361	150 °C	356:361	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	2	18	dep	temperature	330:340	arg1	100					347:349	100	347:349	100	347:349	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	5	19	theme	size	738:741	arg1	distribution					743:754	the particle size distribution	725:754	the particle size distribution of the material collected at different zones	725:799	Sugar yield, crystallinity index, and the particle size distribution of the material collected at different zones were determined.					
30594836	0	20	theme	lignocellulosic	14:28	arg1	biomass					30:36	lignocellulosic biomass	14:36	lignocellulosic biomass	14:36	Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder.					
30594836	0	21	theme	twin-screw	104:113	arg1	extruder					115:122	a twin-screw extruder	102:122	a twin-screw extruder	102:122	Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder.					
30594836	2	22	theme	speed	374:378	arg1	impact					304:309	the impact	300:309	the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm)	300:399	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	4	23	theme	shear	567:571	arg1	influence					554:562	the influence	550:562	the influence of shear imparted by the screws	550:594	To examine the influence of shear imparted by the screws, the residuals samples were recovered from different zones along the screws and evaluated.					
30594836	3	24	theme	increased	515:523	arg1	yields					531:536	increased sugar yields	515:536	increased sugar yields	515:536	Lower temperatures and screw speeds resulted in increased sugar yields.					
30594836	5	25	theme	different	785:793	arg1	zones					795:799	different zones	785:799	different zones	785:799	Sugar yield, crystallinity index, and the particle size distribution of the material collected at different zones were determined.					
30594836	2	26	theme	screw	368:372	arg1	speed					374:378	screw speed	368:378	screw speed (25, 50, and 75 rpm)	368:399	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	6	27	theme	material	865:872	arg1	yield					826:830	Glucose yield	818:830	Glucose yield	818:830	Glucose yield and xylose/mannose yields of the material along the screws, ranged from 23.25 to 42.88% and from 11.95 to 20.54%, respectively.					
30594836	6	27	theme	material	865:872	arg1	yields					851:856	xylose/mannose yields	836:856	xylose/mannose yields	836:856	Glucose yield and xylose/mannose yields of the material along the screws, ranged from 23.25 to 42.88% and from 11.95 to 20.54%, respectively.					
30594836	2	28	theme	sugar	454:458	arg1	yield					460:464	sugar yield	454:464	sugar yield	454:464	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	3	29	theme	sugar	525:529	arg1	yields					531:536	increased sugar yields	515:536	increased sugar yields	515:536	Lower temperatures and screw speeds resulted in increased sugar yields.					
30594836	5	30	theme	crystallinity	700:712	arg1	index					714:718	crystallinity index	700:718	crystallinity index	700:718	Sugar yield, crystallinity index, and the particle size distribution of the material collected at different zones were determined.					
30594836	2	31	theme	temperature	330:340	arg1	impact					304:309	the impact	300:309	the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm)	300:399	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	2	32	dep	speed	374:378	arg1	50					385:386	50	385:386	50	385:386	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	2	32	dep	speed	374:378	arg1	75 rpm					393:398	75 rpm	393:398	75 rpm	393:398	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	2	32	dep	speed	374:378	arg1	25					381:382	25	381:382	25	381:382	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	2	33	theme	barrel	323:328	arg1	temperature					330:340	extruder barrel temperature	314:340	extruder barrel temperature (50, 100, and 150 °C)	314:362	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	2	34	theme	extruder	314:321	arg1	temperature					330:340	extruder barrel temperature	314:340	extruder barrel temperature (50, 100, and 150 °C)	314:362	Initially, the impact of extruder barrel temperature (50, 100, and 150 °C) and screw speed (25, 50, and 75 rpm) were studied to arrive at the optimum conditions for sugar yield.					
30594836	0	35	from	elements	90:97	arg1	extruder					115:122	a twin-screw extruder	102:122	a twin-screw extruder	102:122	Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder.					
30594836	1	36	theme	Proper	125:130	arg1	design					138:143	Proper screw design	125:143	Proper screw design	125:143	Proper screw design is crucial for effectively pre-treating wood fibers, to assist in the downstream enzymatic conversion of the cellulose into fermentable sugars.					
30594836	4	37	theme	different	639:647	arg1	zones					649:653	different zones	639:653	different zones along the screws	639:670	To examine the influence of shear imparted by the screws, the residuals samples were recovered from different zones along the screws and evaluated.					
30594836	0	38	theme	screws	62:67	arg1	length					48:53	the length	44:53	the length of the screws with different screw elements in a twin-screw extruder	44:122	Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder.					
30594836	0	39	with	length	48:53	arg1	elements					90:97	different screw elements	74:97	different screw elements in a twin-screw extruder	74:122	Disruption of lignocellulosic biomass along the length of the screws with different screw elements in a twin-screw extruder.					
30594836	1	40	theme	screw	132:136	arg1	design					138:143	Proper screw design	125:143	Proper screw design	125:143	Proper screw design is crucial for effectively pre-treating wood fibers, to assist in the downstream enzymatic conversion of the cellulose into fermentable sugars.					
30594836	1	41	theme	downstream	215:224	arg1	conversion					236:245	the downstream enzymatic conversion	211:245	the downstream enzymatic conversion of the cellulose into fermentable sugars	211:286	Proper screw design is crucial for effectively pre-treating wood fibers, to assist in the downstream enzymatic conversion of the cellulose into fermentable sugars.					
30594836	6	42	theme	xylose/mannose	836:849	arg1	yields					851:856	xylose/mannose yields	836:856	xylose/mannose yields	836:856	Glucose yield and xylose/mannose yields of the material along the screws, ranged from 23.25 to 42.88% and from 11.95 to 20.54%, respectively.					
30594836	5	43	theme	particle	729:736	arg1	distribution					743:754	the particle size distribution	725:754	the particle size distribution of the material collected at different zones	725:799	Sugar yield, crystallinity index, and the particle size distribution of the material collected at different zones were determined.					
30594836	1	44	theme	enzymatic	226:234	arg1	conversion					236:245	the downstream enzymatic conversion	211:245	the downstream enzymatic conversion of the cellulose into fermentable sugars	211:286	Proper screw design is crucial for effectively pre-treating wood fibers, to assist in the downstream enzymatic conversion of the cellulose into fermentable sugars.					
29699649	1	0	theme	pH-induced	309:318	arg1	perturbations					335:347	the pH-induced conformational perturbations	305:347	the pH-induced conformational perturbations of ovalbumin	305:360	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	1	1	theme	GA	150:151	arg1	coacervates					162:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	1	2	theme	conformational	320:333	arg1	perturbations					335:347	the pH-induced conformational perturbations	305:347	the pH-induced conformational perturbations of ovalbumin	305:360	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	1	3	theme	protein	230:236	arg1	temperature					251:261	the protein denaturation temperature	226:261	the protein denaturation temperature	226:261	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	5	4	theme	OVA	848:850	arg1	ratio					855:859	OVA:GA ratio	848:859	OVA:GA ratio	848:859	Moreover, the salt concentration and OVA:GA ratio influenced the rheological properties by affecting the structure and composition of the complexes.					
29699649	1	5	theme	denaturation	238:249	arg1	temperature					251:261	the protein denaturation temperature	226:261	the protein denaturation temperature	226:261	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	5	6	theme	GA	852:853	arg1	ratio					855:859	OVA:GA ratio	848:859	OVA:GA ratio	848:859	Moreover, the salt concentration and OVA:GA ratio influenced the rheological properties by affecting the structure and composition of the complexes.					
29699649	3	7	dep	showed	541:546	arg1	2.7					643:645	2.7	643:645	2.7	643:645	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	3	8	from	pH	579:580	arg1	value					570:574	the highest viscosity value	548:574	the highest viscosity value at pH 3.7	548:584	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	2	9	theme	random	484:489	arg1	coil					491:494	the random coil	480:494	the random coil	480:494	The presence of GA improved the stability of the α-helix and β-turn regions against pH, but showed less influence on the random coil and β-sheet domains.					
29699649	1	10	theme	ovalbumin	352:360	arg1	perturbations					335:347	the pH-induced conformational perturbations	305:347	the pH-induced conformational perturbations of ovalbumin	305:360	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	1	11	theme	complex	154:160	arg1	coacervates					162:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	1	12	dep	96 °C.	284:289	arg1	to					281:282	to	281:282	to	281:282	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	0	13	theme	Structural	0:9	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and rheological properties of ovalbumin-gum arabic complex coacervates.					
29699649	1	14	theme	coacervates	162:172	arg1	formation					105:113	formation	105:113	formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates	105:172	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	2	15	theme	regions	431:437	arg1	stability					395:403	the stability	391:403	the stability of the α-helix and β-turn regions	391:437	The presence of GA improved the stability of the α-helix and β-turn regions against pH, but showed less influence on the random coil and β-sheet domains.					
29699649	5	16	theme	salt	825:828	arg1	concentration					830:842	the salt concentration	821:842	the salt concentration	821:842	Moreover, the salt concentration and OVA:GA ratio influenced the rheological properties by affecting the structure and composition of the complexes.					
29699649	2	17	theme	β-turn	424:429	arg1	regions					431:437	the α-helix and β-turn regions	408:437	the α-helix and β-turn regions	408:437	The presence of GA improved the stability of the α-helix and β-turn regions against pH, but showed less influence on the random coil and β-sheet domains.					
29699649	0	18	theme	rheological	31:41	arg1	properties					43:52	rheological properties	31:52	rheological properties	31:52	Structural characteristics and rheological properties of ovalbumin-gum arabic complex coacervates.					
29699649	6	19	theme	stronger	962:969	arg1	interaction					971:981	A stronger interaction	960:981	A stronger interaction between OVA and GA	960:1000	A stronger interaction between OVA and GA led to greater viscoelastic properties.					
29699649	3	20	theme	complex	521:527	arg1	coacervates					529:539	The complex coacervates	517:539	The complex coacervates	517:539	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	2	21	theme	α-helix	412:418	arg1	regions					431:437	the α-helix and β-turn regions	408:437	the α-helix and β-turn regions	408:437	The presence of GA improved the stability of the α-helix and β-turn regions against pH, but showed less influence on the random coil and β-sheet domains.					
29699649	3	22	theme	pH	610:611	arg1	values					613:618	the other pH values	600:618	the other pH values tested	600:625	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	1	23	theme	ovalbumin	122:130	arg1	coacervates					162:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	1	24	theme	ordered	182:188	arg1	structure					198:206	a more ordered crystal structure	175:206	a more ordered crystal structure	175:206	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	3	25	theme	stronger	659:666	arg1	interactions					668:679	the stronger interactions	655:679	the stronger interactions of OVA and GA	655:693	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	1	26	theme	crystal	190:196	arg1	structure					198:206	a more ordered crystal structure	175:206	a more ordered crystal structure	175:206	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	5	27	dep	structure	916:924	arg1	the					912:914	the	912:914	the	912:914	Moreover, the salt concentration and OVA:GA ratio influenced the rheological properties by affecting the structure and composition of the complexes.					
29699649	3	28	theme	GA	692:693	arg1	interactions					668:679	the stronger interactions	655:679	the stronger interactions of OVA and GA	655:693	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	2	29	theme	β-sheet	500:506	arg1	domains					508:514	β-sheet domains	500:514	β-sheet domains	500:514	The presence of GA improved the stability of the α-helix and β-turn regions against pH, but showed less influence on the random coil and β-sheet domains.					
29699649	3	30	theme	other	604:608	arg1	values					613:618	the other pH values	600:618	the other pH values tested	600:625	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	3	31	theme	highest	552:558	arg1	value					570:574	the highest viscosity value	548:574	the highest viscosity value at pH 3.7	548:584	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	6	32	theme	viscoelastic	1017:1028	arg1	properties					1030:1039	greater viscoelastic properties	1009:1039	greater viscoelastic properties	1009:1039	A stronger interaction between OVA and GA led to greater viscoelastic properties.					
29699649	0	33	theme	ovalbumin-gum	57:69	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics and rheological properties of ovalbumin-gum arabic complex coacervates.					
29699649	0	33	theme	ovalbumin-gum	57:69	arg1	properties					43:52	rheological properties	31:52	rheological properties	31:52	Structural characteristics and rheological properties of ovalbumin-gum arabic complex coacervates.					
29699649	3	34	theme	OVA	684:686	arg1	interactions					668:679	the stronger interactions	655:679	the stronger interactions of OVA and GA	655:693	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	3	35	theme	viscosity	560:568	arg1	value					570:574	the highest viscosity value	548:574	the highest viscosity value at pH 3.7	548:584	The complex coacervates showed the highest viscosity value at pH 3.7 compared with the other pH values tested (4.0, 3.4, 3.0, 2.7) due to the stronger interactions of OVA and GA.					
29699649	6	36	theme	greater	1009:1015	arg1	properties					1030:1039	greater viscoelastic properties	1009:1039	greater viscoelastic properties	1009:1039	A stronger interaction between OVA and GA led to greater viscoelastic properties.					
29699649	4	37	theme	large	698:702	arg1	loop					716:719	A large thixotropic loop	696:719	A large thixotropic loop	696:719	A large thixotropic loop was observed for the coacervate obtained at pH 3.7 compared with that obtained at pH 4.0.					
29699649	5	38	theme	rheological	876:886	arg1	properties					888:897	the rheological properties	872:897	the rheological properties	872:897	Moreover, the salt concentration and OVA:GA ratio influenced the rheological properties by affecting the structure and composition of the complexes.					
29699649	1	39	theme	-gum	137:140	arg1	coacervates					162:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	5	40	theme	complexes	949:957	arg1	composition					930:940	composition	930:940	composition	930:940	Moreover, the salt concentration and OVA:GA ratio influenced the rheological properties by affecting the structure and composition of the complexes.					
29699649	5	40	theme	complexes	949:957	arg1	structure					916:924	structure	916:924	structure	916:924	Moreover, the salt concentration and OVA:GA ratio influenced the rheological properties by affecting the structure and composition of the complexes.					
29699649	1	41	theme	arabic	142:147	arg1	coacervates					162:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	the ovalbumin (OVA)-gum arabic (GA) complex coacervates	118:172	After formation of the ovalbumin (OVA)-gum arabic (GA) complex coacervates, a more ordered crystal structure was obtained, and the protein denaturation temperature increased from 72 to 96 °C. GA can reduce the pH-induced conformational perturbations of ovalbumin.					
29699649	2	42	theme	GA	379:380	arg1	presence					367:374	The presence	363:374	The presence of GA	363:380	The presence of GA improved the stability of the α-helix and β-turn regions against pH, but showed less influence on the random coil and β-sheet domains.					
29699649	0	43	theme	complex	78:84	arg1	coacervates					86:96	complex coacervates	78:96	complex coacervates	78:96	Structural characteristics and rheological properties of ovalbumin-gum arabic complex coacervates.					
29699649	4	44	theme	thixotropic	704:714	arg1	loop					716:719	A large thixotropic loop	696:719	A large thixotropic loop	696:719	A large thixotropic loop was observed for the coacervate obtained at pH 3.7 compared with that obtained at pH 4.0.					
31028810	0	0	theme	dressing	122:129	arg1	applications					131:142	potential wound dressing applications	106:142	potential wound dressing applications	106:142	Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications.					
31028810	3	1	theme	constituents	710:721	arg1	groups					696:701	functional groups	685:701	functional groups of the constituents	685:721	FTIR results indicated that hydrogen bonding has been formed between functional groups of the constituents.					
31028810	3	1	theme	constituents	710:721	arg1	constituents					710:721	the constituents	706:721	the constituents	706:721	FTIR results indicated that hydrogen bonding has been formed between functional groups of the constituents.					
31028810	2	2	theme	oxygen	577:582	arg1	release					584:590	oxygen release	577:590	oxygen release	577:590	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	8	3	theme	cell	1438:1441	arg1	death					1443:1447	cell death	1438:1447	cell death	1438:1447	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	6	4	theme	antibacterial	1030:1042	arg1	activity					1044:1051	the antibacterial activity	1026:1051	the antibacterial activity of the films against E. coli bacteria	1026:1089	By Adding CP to the film composition, the antibacterial activity of the films against E. coli bacteria increased.					
31028810	4	5	contain	had	794:796	arg2	results					805:811	better results	798:811	better results	798:811	The mechanical results showed that the combination of both CP and NCC had better results in improving the mechanical properties of the films.					
31028810	4	5	contain	had	794:796	arg1	combination					763:773	the combination	759:773	the combination of both CP and NCC	759:792	The mechanical results showed that the combination of both CP and NCC had better results in improving the mechanical properties of the films.					
31028810	0	6	theme	wound	116:120	arg1	applications					131:142	potential wound dressing applications	106:142	potential wound dressing applications	106:142	Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications.					
31028810	4	7	theme	mechanical	830:839	arg1	properties					841:850	the mechanical properties	826:850	the mechanical properties of the films	826:863	The mechanical results showed that the combination of both CP and NCC had better results in improving the mechanical properties of the films.					
31028810	5	8	theme	WVTR	870:873	arg1	results					888:894	The WVTR and swelling results	866:894	The WVTR and swelling results	866:894	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	1	9	theme	calcium	296:302	arg1	CP					314:315	CP	314:315	CP	314:315	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	1	9	theme	calcium	296:302	arg1	peroxide					304:311	calcium peroxide	296:311	calcium peroxide (CP)	296:316	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	2	10	theme	Fourier-transform	470:486	arg1	swelling					514:521	Fourier-transform infrared (FTIR), tensile, swelling	470:521	swelling	514:521	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	5	11	theme	samples	979:985	arg1	swelling					963:970	swelling	963:970	swelling	963:970	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	5	11	theme	samples	979:985	arg1	WVTR					954:957	WVTR	954:957	WVTR	954:957	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	1	12	theme	peroxide	304:311	arg1	particles					318:326	nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles	260:326	nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles	260:326	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	8	13	theme	assay	1248:1252	arg1	results					1254:1260	The MTT assay results	1240:1260	The MTT assay results	1240:1260	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	2	14	theme	culture	601:607	arg1	tests					609:613	cell culture tests	596:613	cell culture tests	596:613	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	6	15	theme	film	1008:1011	arg1	composition					1013:1023	the film composition	1004:1023	the film composition	1004:1023	By Adding CP to the film composition, the antibacterial activity of the films against E. coli bacteria increased.					
31028810	1	16	theme	chitosan-based	214:227	arg1	films					239:243	chitosan-based polymeric films	214:243	chitosan-based polymeric films	214:243	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	3	17	theme	functional	685:694	arg1	groups					696:701	functional groups	685:701	functional groups of the constituents	685:721	FTIR results indicated that hydrogen bonding has been formed between functional groups of the constituents.					
31028810	3	17	theme	functional	685:694	arg1	constituents					710:721	the constituents	706:721	the constituents	706:721	FTIR results indicated that hydrogen bonding has been formed between functional groups of the constituents.					
31028810	2	18	theme	cell	596:599	arg1	tests					609:613	cell culture tests	596:613	cell culture tests	596:613	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	1	19	theme	polymeric	229:237	arg1	films					239:243	chitosan-based polymeric films	214:243	chitosan-based polymeric films	214:243	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	2	20	theme	infrared	488:495	arg1	swelling					514:521	Fourier-transform infrared (FTIR), tensile, swelling	470:521	swelling	514:521	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	7	21	theme	maximum	1157:1163	arg1	value					1165:1169	its maximum value	1153:1169	its maximum value	1153:1169	The oxygen release for the films containing CP has its maximum value during the first day and it approaches a constant value for 10 days.					
31028810	4	22	theme	films	859:863	arg1	properties					841:850	the mechanical properties	826:850	the mechanical properties of the films	826:863	The mechanical results showed that the combination of both CP and NCC had better results in improving the mechanical properties of the films.					
31028810	1	23	theme	films	239:243	arg1	properties					176:185	mechanical properties	165:185	mechanical properties	165:185	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	1	23	theme	films	239:243	arg1	behavior					202:209	biomedical behavior	191:209	biomedical behavior	191:209	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	6	24	dep	E.	1074:1075	arg1	coli					1077:1080	coli	1077:1080	coli	1077:1080	By Adding CP to the film composition, the antibacterial activity of the films against E. coli bacteria increased.					
31028810	2	25	theme	transmission	536:547	arg1	WVTR					555:558	WVTR	555:558	WVTR	555:558	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	2	25	theme	transmission	536:547	arg1	rate					549:552	water vapor transmission rate	524:552	water vapor transmission rate (WVTR)	524:559	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	4	26	theme	mechanical	728:737	arg1	results					739:745	The mechanical results	724:745	The mechanical results	724:745	The mechanical results showed that the combination of both CP and NCC had better results in improving the mechanical properties of the films.					
31028810	8	27	theme	cells	1311:1315	arg1	growth					1280:1285	the growth	1276:1285	the growth of the human fibroblast cells	1276:1315	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	7	28	theme	oxygen	1106:1111	arg1	release					1113:1119	The oxygen release	1102:1119	The oxygen release for the films containing CP	1102:1147	The oxygen release for the films containing CP has its maximum value during the first day and it approaches a constant value for 10 days.					
31028810	1	29	theme	particles	318:326	arg1	effect					250:255	the effect	246:255	the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL)	246:403	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	2	30	theme	vapor	530:534	arg1	WVTR					555:558	WVTR	555:558	WVTR	555:558	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	2	30	theme	vapor	530:534	arg1	rate					549:552	water vapor transmission rate	524:552	water vapor transmission rate (WVTR)	524:559	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	3	31	theme	FTIR	616:619	arg1	results					621:627	FTIR results	616:627	FTIR results	616:627	FTIR results indicated that hydrogen bonding has been formed between functional groups of the constituents.					
31028810	5	32	theme	NCC	918:920	arg1	particles					922:930	NCC particles	918:930	NCC particles	918:930	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	0	33	theme	cellulose/calcium	69:85	arg1	films					96:100	chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films	36:100	chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films	36:100	Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications.					
31028810	8	34	theme	MTT	1244:1246	arg1	results					1254:1260	The MTT assay results	1240:1260	The MTT assay results	1240:1260	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	2	35	theme	water	524:528	arg1	WVTR					555:558	WVTR	555:558	WVTR	555:558	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	2	35	theme	water	524:528	arg1	rate					549:552	water vapor transmission rate	524:552	water vapor transmission rate (WVTR)	524:559	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	4	36	theme	NCC	790:792	arg1	combination					763:773	the combination	759:773	the combination of both CP and NCC	759:792	The mechanical results showed that the combination of both CP and NCC had better results in improving the mechanical properties of the films.					
31028810	7	37	theme	constant	1212:1219	arg1	value					1221:1225	a constant value	1210:1225	a constant value for 10 days	1210:1237	The oxygen release for the films containing CP has its maximum value during the first day and it approaches a constant value for 10 days.					
31028810	0	38	theme	chitosan/gelatin/nanocrystalline	36:67	arg1	films					96:100	chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films	36:100	chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films	36:100	Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications.					
31028810	8	39	theme	fibroblast	1300:1309	arg1	cells					1311:1315	the human fibroblast cells	1290:1315	the human fibroblast cells	1290:1315	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	1	40	theme	nanocrystalline	260:274	arg1	NCC					287:289	NCC	287:289	NCC	287:289	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	1	40	theme	nanocrystalline	260:274	arg1	cellulose					276:284	nanocrystalline cellulose	260:284	nanocrystalline cellulose (NCC)	260:290	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	0	41	theme	films	96:100	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications.					
31028810	0	41	theme	films	96:100	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications.					
31028810	8	42	theme	chitosan-based	1363:1376	arg1	films					1378:1382	the chitosan-based films	1359:1382	the chitosan-based films containing CP and NCC	1359:1404	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	4	43	theme	better	798:803	arg1	results					805:811	better results	798:811	better results	798:811	The mechanical results showed that the combination of both CP and NCC had better results in improving the mechanical properties of the films.					
31028810	6	44	theme	E.	1074:1075	arg1	bacteria					1082:1089	E. coli bacteria	1074:1089	E. coli bacteria	1074:1089	By Adding CP to the film composition, the antibacterial activity of the films against E. coli bacteria increased.					
31028810	0	45	theme	peroxide	87:94	arg1	films					96:100	chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films	36:100	chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films	36:100	Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications.					
31028810	2	46	dep	infrared	488:495	arg1	FTIR					498:501	FTIR	498:501	FTIR	498:501	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	2	46	dep	infrared	488:495	arg1	tensile					505:511	tensile	505:511	tensile	505:511	The films were characterized by Fourier-transform infrared (FTIR), tensile, swelling, water vapor transmission rate (WVTR), antibacterial, oxygen release and cell culture tests.					
31028810	3	47	theme	hydrogen	644:651	arg1	bonding					653:659	hydrogen bonding	644:659	hydrogen bonding	644:659	FTIR results indicated that hydrogen bonding has been formed between functional groups of the constituents.					
31028810	5	48	theme	swelling	879:886	arg1	results					888:894	The WVTR and swelling results	866:894	The WVTR and swelling results	866:894	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	1	49	theme	polymeric	349:357	arg1	films					359:363	polymeric films	349:363	polymeric films based on chitosan (CS) and gelatin (GL)	349:403	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	1	50	from	effect	250:255	arg1	properties					335:344	the properties	331:344	the properties of polymeric films based on chitosan (CS) and gelatin (GL)	331:403	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	1	51	theme	mechanical	165:174	arg1	properties					176:185	mechanical properties	165:185	mechanical properties	165:185	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	8	52	theme	human	1294:1298	arg1	cells					1311:1315	the human fibroblast cells	1290:1315	the human fibroblast cells	1290:1315	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	1	53	theme	cellulose	276:284	arg1	particles					318:326	nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles	260:326	nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles	260:326	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	7	54	contain	containing	1135:1144	arg2	CP					1146:1147	CP	1146:1147	CP	1146:1147	The oxygen release for the films containing CP has its maximum value during the first day and it approaches a constant value for 10 days.					
31028810	7	54	contain	containing	1135:1144	arg1	films					1129:1133	the films	1125:1133	the films containing CP	1125:1147	The oxygen release for the films containing CP has its maximum value during the first day and it approaches a constant value for 10 days.					
31028810	5	55	theme	WVTR	954:957	arg1	swelling					963:970	swelling	963:970	swelling	963:970	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	5	55	theme	WVTR	954:957	arg1	WVTR					954:957	WVTR	954:957	WVTR	954:957	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	5	55	theme	WVTR	954:957	arg1	amount					944:949	the amount	940:949	the amount of WVTR and swelling of the samples	940:985	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	8	56	contain	containing	1384:1393	arg2	NCC					1402:1404	NCC	1402:1404	NCC	1402:1404	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	8	56	contain	containing	1384:1393	arg2	CP					1395:1396	CP	1395:1396	CP	1395:1396	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	8	56	contain	containing	1384:1393	arg1	films					1378:1382	the chitosan-based films	1359:1382	the chitosan-based films containing CP and NCC	1359:1404	The MTT assay results revealed that the growth of the human fibroblast cells was increased during 7 days, showing that the chitosan-based films containing CP and NCC had no toxicity and never cause cell death.					
31028810	6	57	theme	films	1060:1064	arg1	activity					1044:1051	the antibacterial activity	1026:1051	the antibacterial activity of the films against E. coli bacteria	1026:1089	By Adding CP to the film composition, the antibacterial activity of the films against E. coli bacteria increased.					
31028810	0	58	theme	potential	106:114	arg1	applications					131:142	potential wound dressing applications	106:142	potential wound dressing applications	106:142	Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications.					
31028810	4	59	theme	CP	783:784	arg1	combination					763:773	the combination	759:773	the combination of both CP and NCC	759:792	The mechanical results showed that the combination of both CP and NCC had better results in improving the mechanical properties of the films.					
31028810	7	60	contain	has	1149:1151	arg2	value					1165:1169	its maximum value	1153:1169	its maximum value	1153:1169	The oxygen release for the films containing CP has its maximum value during the first day and it approaches a constant value for 10 days.					
31028810	7	60	contain	has	1149:1151	arg1	release					1113:1119	The oxygen release	1102:1119	The oxygen release for the films containing CP	1102:1147	The oxygen release for the films containing CP has its maximum value during the first day and it approaches a constant value for 10 days.					
31028810	7	61	theme	first	1182:1186	arg1	day					1188:1190	the first day	1178:1190	the first day	1178:1190	The oxygen release for the films containing CP has its maximum value during the first day and it approaches a constant value for 10 days.					
31028810	1	62	theme	films	359:363	arg1	properties					335:344	the properties	331:344	the properties of polymeric films based on chitosan (CS) and gelatin (GL)	331:403	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
31028810	5	63	theme	swelling	963:970	arg1	swelling					963:970	swelling	963:970	swelling	963:970	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	5	63	theme	swelling	963:970	arg1	WVTR					954:957	WVTR	954:957	WVTR	954:957	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	5	63	theme	swelling	963:970	arg1	amount					944:949	the amount	940:949	the amount of WVTR and swelling of the samples	940:985	The WVTR and swelling results indicated that CP and NCC particles reduced the amount of WVTR and swelling of the samples.					
31028810	1	64	theme	biomedical	191:200	arg1	behavior					202:209	biomedical behavior	191:209	biomedical behavior	191:209	In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study.					
30635335	5	0	theme	main	761:764	arg1	components					766:775	main components	761:775	main components of the outer membrane	761:797	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	2	1	theme	essential	370:378	arg1	Omp2b					401:405	the essential outer membrane porin Omp2b	366:405	the essential outer membrane porin Omp2b	366:405	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	5	2	theme	components	766:775	arg1	absence					726:732	The absence	722:732	The absence of long-range diffusion of main components of the outer membrane	722:797	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	0	3	from	incorporation	10:22	arg1	abortus					78:84	the pathogen Brucella abortus	56:84	the pathogen Brucella abortus	56:84	Localized incorporation of outer membrane components in the pathogen Brucella abortus.					
30635335	3	4	theme	growth	631:636	arg1	sites					638:642	the expected growth sites	618:642	the expected growth sites	618:642	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.					
30635335	3	5	theme	outer	515:519	arg1	Omp25					538:542	the major outer membrane protein Omp25	505:542	the major outer membrane protein Omp25	505:542	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.					
30635335	5	6	theme	lipopolysaccharide	957:974	arg1	localizations					893:905	unipolar and mid-cell localizations	871:905	unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide	871:974	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	5	6	theme	lipopolysaccharide	957:974	arg1	clusters					850:857	the Omp2b clusters	840:857	the Omp2b clusters	840:857	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	6	7	theme	surface	1150:1156	arg1	antigens					1158:1165	diluting pre-existing surface antigens	1128:1165	diluting pre-existing surface antigens	1128:1165	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	6	8	theme	limited	997:1003	arg1	mobility					1005:1012	limited mobility	997:1012	limited mobility	997:1012	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	2	9	theme	membrane	283:290	arg1	composition					292:302	its outer membrane composition	273:302	its outer membrane composition	273:302	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	5	10	theme	incorporated	916:927	arg1	proteins					944:951	newly incorporated outer membrane proteins	910:951	newly incorporated outer membrane proteins	910:951	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	5	11	theme	localizations	893:905	arg1	immobility					826:835	the apparent immobility	813:835	the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide	813:974	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	5	12	theme	membrane	935:942	arg1	proteins					944:951	newly incorporated outer membrane proteins	910:951	newly incorporated outer membrane proteins	910:951	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	6	13	theme	surface	1058:1064	arg1	variants					1066:1073	new surface variants	1054:1073	new surface variants	1054:1073	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	2	14	theme	outer	277:281	arg1	composition					292:302	its outer membrane composition	273:302	its outer membrane composition	273:302	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	5	15	theme	clusters	850:857	arg1	immobility					826:835	the apparent immobility	813:835	the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide	813:974	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	5	16	theme	long-range	737:746	arg1	diffusion					748:756	long-range diffusion	737:756	long-range diffusion	737:756	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	5	17	theme	mid-cell	884:891	arg1	localizations					893:905	unipolar and mid-cell localizations	871:905	unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide	871:974	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	6	18	theme	few	1092:1094	arg1	generations					1096:1106	a few generations	1090:1106	a few generations	1090:1106	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	3	19	dep	pole	590:593	arg1	sites					638:642	the expected growth sites	618:642	the expected growth sites	618:642	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.					
30635335	2	20	theme	solutes	460:466	arg1	diffusion					447:455	facilitated diffusion	435:455	facilitated diffusion of solutes	435:466	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	3	21	theme	membrane	521:528	arg1	Omp25					538:542	the major outer membrane protein Omp25	505:542	the major outer membrane protein Omp25	505:542	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.					
30635335	5	22	theme	diffusion	748:756	arg1	absence					726:732	The absence	722:732	The absence of long-range diffusion of main components of the outer membrane	722:797	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	5	23	theme	outer	929:933	arg1	proteins					944:951	newly incorporated outer membrane proteins	910:951	newly incorporated outer membrane proteins	910:951	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	0	24	theme	Localized	0:8	arg1	incorporation					10:22	Localized incorporation	0:22	Localized incorporation of outer membrane components in the pathogen Brucella abortus.	0:85	Localized incorporation of outer membrane components in the pathogen Brucella abortus.					
30635335	1	25	theme	zoonotic	91:98	arg1	part					129:132	part	129:132	part	129:132	The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth.					
30635335	1	25	theme	zoonotic	91:98	arg1	pathogen					100:107	The zoonotic pathogen	87:107	The zoonotic pathogen Brucella abortus	87:124	The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth.					
30635335	2	26	theme	porin	395:399	arg1	Omp2b					401:405	the essential outer membrane porin Omp2b	366:405	the essential outer membrane porin Omp2b	366:405	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	0	27	theme	outer	27:31	arg1	components					42:51	outer membrane components	27:51	outer membrane components	27:51	Localized incorporation of outer membrane components in the pathogen Brucella abortus.					
30635335	6	28	theme	Unipolar	977:984	arg1	growth					986:991	Unipolar growth	977:991	Unipolar growth	977:991	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	5	29	theme	proteins	944:951	arg1	localizations					893:905	unipolar and mid-cell localizations	871:905	unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide	871:974	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	5	29	theme	proteins	944:951	arg1	clusters					850:857	the Omp2b clusters	840:857	the Omp2b clusters	840:857	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	2	30	theme	facilitated	435:445	arg1	diffusion					447:455	facilitated diffusion	435:455	facilitated diffusion of solutes	435:466	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	3	31	theme	protein	530:536	arg1	Omp25					538:542	the major outer membrane protein Omp25	505:542	the major outer membrane protein Omp25	505:542	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.					
30635335	2	32	from	heterogeneity	256:268	arg1	composition					292:302	its outer membrane composition	273:302	its outer membrane composition	273:302	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	1	33	dep	pathogen	100:107	arg1	abortus					118:124	Brucella abortus	109:124	The zoonotic pathogen Brucella abortus	87:124	The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth.					
30635335	0	34	theme	components	42:51	arg1	incorporation					10:22	Localized incorporation	0:22	Localized incorporation of outer membrane components in the pathogen Brucella abortus.	0:85	Localized incorporation of outer membrane components in the pathogen Brucella abortus.					
30635335	4	35	theme	same	703:706	arg1	sites					715:719	the same growth sites	699:719	the same growth sites	699:719	Interestingly, lipopolysaccharide is also inserted at the same growth sites.					
30635335	6	36	theme	diluting	1128:1135	arg1	antigens					1158:1165	diluting pre-existing surface antigens	1128:1165	diluting pre-existing surface antigens	1128:1165	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	1	37	theme	unipolar	196:203	arg1	growth					205:210	unipolar growth	196:210	unipolar growth	196:210	The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth.					
30635335	6	38	theme	new	1054:1056	arg1	variants					1066:1073	new surface variants	1054:1073	new surface variants	1054:1073	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	0	39	theme	membrane	33:40	arg1	components					42:51	outer membrane components	27:51	outer membrane components	27:51	Localized incorporation of outer membrane components in the pathogen Brucella abortus.					
30635335	3	40	theme	expected	622:629	arg1	sites					638:642	the expected growth sites	618:642	the expected growth sites	618:642	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.					
30635335	5	41	theme	Omp2b	844:848	arg1	clusters					850:857	the Omp2b clusters	840:857	the Omp2b clusters	840:857	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	5	42	theme	outer	784:788	arg1	membrane					790:797	the outer membrane	780:797	the outer membrane	780:797	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	2	43	with	co-localizing	347:359	arg1	Omp2b					401:405	the essential outer membrane porin Omp2b	366:405	the essential outer membrane porin Omp2b	366:405	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	3	44	theme	new	586:588	arg1	pole					590:593	the new pole	582:593	the new pole	582:593	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.					
30635335	4	45	theme	growth	708:713	arg1	sites					715:719	the same growth sites	699:719	the same growth sites	699:719	Interestingly, lipopolysaccharide is also inserted at the same growth sites.					
30635335	5	46	theme	membrane	790:797	arg1	components					766:775	main components	761:775	main components of the outer membrane	761:797	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	1	47	theme	Rhizobiales	141:151	arg1	part					129:132	part	129:132	part	129:132	The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth.					
30635335	1	47	theme	Rhizobiales	141:151	arg1	pathogen					100:107	The zoonotic pathogen	87:107	The zoonotic pathogen Brucella abortus	87:124	The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth.					
30635335	1	47	theme	Rhizobiales	141:151	arg1	alpha-proteobacteria					164:183	alpha-proteobacteria	164:183	alpha-proteobacteria displaying unipolar growth	164:210	The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth.					
30635335	3	48	theme	major	509:513	arg1	Omp25					538:542	the major outer membrane protein Omp25	505:542	the major outer membrane protein Omp25	505:542	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.					
30635335	2	49	theme	co-localizing	347:359	arg1	clusters					310:317	clusters	310:317	clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin	310:484	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	2	50	theme	lipopolysaccharide	328:345	arg1	clusters					310:317	clusters	310:317	clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin	310:484	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	6	51	theme	antigens	1158:1165	arg1	need					1120:1123	the need	1116:1123	the need of diluting pre-existing surface antigens	1116:1165	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	2	52	theme	rough	322:326	arg1	lipopolysaccharide					328:345	rough lipopolysaccharide	322:345	rough lipopolysaccharide	322:345	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	6	53	theme	pre-existing	1137:1148	arg1	antigens					1158:1165	diluting pre-existing surface antigens	1128:1165	diluting pre-existing surface antigens	1128:1165	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	0	54	theme	Brucella	69:76	arg1	abortus					78:84	the pathogen Brucella abortus	56:84	the pathogen Brucella abortus	56:84	Localized incorporation of outer membrane components in the pathogen Brucella abortus.					
30635335	5	55	theme	unipolar	871:878	arg1	localizations					893:905	unipolar and mid-cell localizations	871:905	unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide	871:974	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	3	56	theme	division	603:610	arg1	site					612:615	the division site	599:615	the division site	599:615	We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites.					
30635335	2	57	theme	membrane	386:393	arg1	Omp2b					401:405	the essential outer membrane porin Omp2b	366:405	the essential outer membrane porin Omp2b	366:405	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	6	58	theme	structures	1025:1034	arg1	mobility					1005:1012	limited mobility	997:1012	limited mobility	997:1012	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	6	58	theme	structures	1025:1034	arg1	growth					986:991	Unipolar growth	977:991	Unipolar growth	977:991	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
30635335	0	59	theme	pathogen	60:67	arg1	abortus					78:84	the pathogen Brucella abortus	56:84	the pathogen Brucella abortus	56:84	Localized incorporation of outer membrane components in the pathogen Brucella abortus.					
30635335	5	60	theme	apparent	817:824	arg1	immobility					826:835	the apparent immobility	813:835	the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide	813:974	The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide.					
30635335	2	61	theme	outer	380:384	arg1	Omp2b					401:405	the essential outer membrane porin Omp2b	366:405	the essential outer membrane porin Omp2b	366:405	Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin.					
30635335	6	62	theme	surface	1017:1023	arg1	structures					1025:1034	surface structures	1017:1034	surface structures	1017:1034	Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.					
29401645	5	0	theme	lesion	722:727	arg1	composition					729:739	lesion composition	722:739	lesion composition	722:739	At week 5, vascular pathology was determined by lesion thickness, vascular remodeling, and lesion composition.					
29401645	8	1	theme	content	1120:1126	arg1	number					1063:1068	increased number	1053:1068	increased number of macrophages, smooth muscle cells, and collagen content	1053:1126	The lesions showed increased number of macrophages, smooth muscle cells, and collagen content.					
29401645	8	2	theme	muscle	1093:1098	arg1	cells					1100:1104	smooth muscle cells	1086:1104	smooth muscle cells	1086:1104	The lesions showed increased number of macrophages, smooth muscle cells, and collagen content.					
29401645	3	3	theme	10	490:491	arg1	%					492:492	%	492:492	%	492:492	Male E3L mice were fed a high-cholesterol (1%) diet, supplemented with or without 10% inulin for 5 weeks.					
29401645	8	4	theme	smooth	1086:1091	arg1	cells					1100:1104	smooth muscle cells	1086:1104	smooth muscle cells	1086:1104	The lesions showed increased number of macrophages, smooth muscle cells, and collagen content.					
29401645	0	5	theme	3-Leiden	89:96	arg1	Mice					98:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	The Prebiotic Inulin Aggravates Accelerated Atherosclerosis in Hypercholesterolemic APOE*3-Leiden Mice.					
29401645	6	6	theme	cytometry	858:866	arg1	analysis					868:875	flow cytometry analysis	853:875	flow cytometry analysis	853:875	Throughout the study, plasma lipids were measured and in week 5, blood monocyte subtypes were determined using flow cytometry analysis.					
29401645	9	7	theme	blood	1143:1147	arg1	composition					1158:1168	blood monocyte composition	1143:1168	blood monocyte composition	1143:1168	No effects on blood monocyte composition were found.					
29401645	2	8	theme	*	386:386	arg1	mice					402:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	4	9	theme	non-constrictive	527:542	arg1	cuff					544:547	a non-constrictive cuff	525:547	a non-constrictive cuff	525:547	At week 3, a non-constrictive cuff was placed around the right femoral artery to induce accelerated atherosclerosis.					
29401645	11	10	theme	E3L	1374:1376	arg1	mice					1378:1381	hypercholesterolemic E3L mice	1353:1381	hypercholesterolemic E3L mice	1353:1381	In conclusion, inulin aggravated accelerated atherosclerosis development in hypercholesterolemic E3L mice, accompanied by adverse lesion composition and outward remodeling.					
29401645	6	11	theme	flow	853:856	arg1	analysis					868:875	flow cytometry analysis	853:875	flow cytometry analysis	853:875	Throughout the study, plasma lipids were measured and in week 5, blood monocyte subtypes were determined using flow cytometry analysis.					
29401645	2	12	from	development	346:356	arg1	mice					402:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	2	13	theme	inulin	304:309	arg1	supplementation					311:325	inulin supplementation	304:325	inulin supplementation	304:325	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	3	14	theme	E3L	413:415	arg1	mice					417:420	Male E3L mice	408:420	Male E3L mice	408:420	Male E3L mice were fed a high-cholesterol (1%) diet, supplemented with or without 10% inulin for 5 weeks.					
29401645	11	15	theme	lesion	1407:1412	arg1	composition					1414:1424	adverse lesion composition	1399:1424	adverse lesion composition	1399:1424	In conclusion, inulin aggravated accelerated atherosclerosis development in hypercholesterolemic E3L mice, accompanied by adverse lesion composition and outward remodeling.					
29401645	6	16	theme	plasma	764:769	arg1	lipids					771:776	plasma lipids	764:776	plasma lipids	764:776	Throughout the study, plasma lipids were measured and in week 5, blood monocyte subtypes were determined using flow cytometry analysis.					
29401645	2	17	theme	3-Leiden	387:394	arg1	mice					402:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	5	18	theme	vascular	697:704	arg1	remodeling					706:715	vascular remodeling	697:715	vascular remodeling	697:715	At week 5, vascular pathology was determined by lesion thickness, vascular remodeling, and lesion composition.					
29401645	10	19	theme	total	1249:1253	arg1	exposure					1267:1274	total cholesterol exposure	1249:1274	total cholesterol exposure	1249:1274	Inulin significantly increased plasma total cholesterol levels and total cholesterol exposure.					
29401645	1	20	theme	lipid	182:186	arg1	levels					188:193	plasma lipid levels	175:193	plasma lipid levels	175:193	The prebiotic inulin has proven effective at lowering inflammation and plasma lipid levels.					
29401645	11	21	theme	outward	1430:1436	arg1	remodeling					1438:1447	outward remodeling	1430:1447	outward remodeling	1430:1447	In conclusion, inulin aggravated accelerated atherosclerosis development in hypercholesterolemic E3L mice, accompanied by adverse lesion composition and outward remodeling.					
29401645	0	22	from	Atherosclerosis	44:58	arg1	Mice					98:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	The Prebiotic Inulin Aggravates Accelerated Atherosclerosis in Hypercholesterolemic APOE*3-Leiden Mice.					
29401645	11	23	theme	hypercholesterolemic	1353:1372	arg1	mice					1378:1381	hypercholesterolemic E3L mice	1353:1381	hypercholesterolemic E3L mice	1353:1381	In conclusion, inulin aggravated accelerated atherosclerosis development in hypercholesterolemic E3L mice, accompanied by adverse lesion composition and outward remodeling.					
29401645	0	24	theme	Prebiotic	4:12	arg1	Inulin					14:19	The Prebiotic Inulin	0:19	The Prebiotic Inulin	0:19	The Prebiotic Inulin Aggravates Accelerated Atherosclerosis in Hypercholesterolemic APOE*3-Leiden Mice.					
29401645	8	25	theme	collagen	1111:1118	arg1	content					1120:1126	collagen content	1111:1126	collagen content	1111:1126	The lesions showed increased number of macrophages, smooth muscle cells, and collagen content.					
29401645	9	26	from	effects	1132:1138	arg1	composition					1158:1168	blood monocyte composition	1143:1168	blood monocyte composition	1143:1168	No effects on blood monocyte composition were found.					
29401645	2	27	theme	atherosclerosis	330:344	arg1	development					346:356	atherosclerosis development	330:356	atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice	330:405	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	12	28	theme	aggravated	1549:1558	arg1	development					1576:1586	the aggravated atherosclerosis development	1545:1586	the aggravated atherosclerosis development	1545:1586	This process was not accompanied by differences in blood monocyte composition, suggesting that the aggravated atherosclerosis development was driven by increased plasma cholesterol.					
29401645	3	29	theme	Male	408:411	arg1	mice					417:420	Male E3L mice	408:420	Male E3L mice	408:420	Male E3L mice were fed a high-cholesterol (1%) diet, supplemented with or without 10% inulin for 5 weeks.					
29401645	9	30	theme	monocyte	1149:1156	arg1	composition					1158:1168	blood monocyte composition	1143:1168	blood monocyte composition	1143:1168	No effects on blood monocyte composition were found.					
29401645	3	31	theme	high-cholesterol	433:448	arg1	diet					455:458	a high-cholesterol (1%) diet	431:458	a high-cholesterol (1%) diet	431:458	Male E3L mice were fed a high-cholesterol (1%) diet, supplemented with or without 10% inulin for 5 weeks.					
29401645	10	32	theme	total	1220:1224	arg1	levels					1238:1243	plasma total cholesterol levels	1213:1243	plasma total cholesterol levels	1213:1243	Inulin significantly increased plasma total cholesterol levels and total cholesterol exposure.					
29401645	2	33	theme	supplementation	311:325	arg1	effect					294:299	the effect	290:299	the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice	290:405	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	7	34	from	exacerbated	916:926	arg1	contrast					881:888	contrast	881:888	contrast to our hypothesis	881:906	In contrast to our hypothesis, inulin exacerbated atherosclerosis development, characterized by increased lesion formation and outward vascular remodeling.					
29401645	8	35	theme	increased	1053:1061	arg1	number					1063:1068	increased number	1053:1068	increased number of macrophages, smooth muscle cells, and collagen content	1053:1126	The lesions showed increased number of macrophages, smooth muscle cells, and collagen content.					
29401645	6	36	theme	monocyte	813:820	arg1	subtypes					822:829	blood monocyte subtypes	807:829	blood monocyte subtypes	807:829	Throughout the study, plasma lipids were measured and in week 5, blood monocyte subtypes were determined using flow cytometry analysis.					
29401645	1	37	theme	plasma	175:180	arg1	levels					188:193	plasma lipid levels	175:193	plasma lipid levels	175:193	The prebiotic inulin has proven effective at lowering inflammation and plasma lipid levels.					
29401645	11	38	theme	atherosclerosis	1322:1336	arg1	development					1338:1348	atherosclerosis development	1322:1348	atherosclerosis development	1322:1348	In conclusion, inulin aggravated accelerated atherosclerosis development in hypercholesterolemic E3L mice, accompanied by adverse lesion composition and outward remodeling.					
29401645	7	39	from	contrast	881:888	arg1	exacerbated					916:926	exacerbated	916:926	exacerbated	916:926	In contrast to our hypothesis, inulin exacerbated atherosclerosis development, characterized by increased lesion formation and outward vascular remodeling.					
29401645	8	40	theme	macrophages	1073:1083	arg1	number					1063:1068	increased number	1053:1068	increased number of macrophages, smooth muscle cells, and collagen content	1053:1126	The lesions showed increased number of macrophages, smooth muscle cells, and collagen content.					
29401645	6	41	theme	blood	807:811	arg1	subtypes					822:829	blood monocyte subtypes	807:829	blood monocyte subtypes	807:829	Throughout the study, plasma lipids were measured and in week 5, blood monocyte subtypes were determined using flow cytometry analysis.					
29401645	4	42	theme	accelerated	602:612	arg1	atherosclerosis					614:628	accelerated atherosclerosis	602:628	accelerated atherosclerosis	602:628	At week 3, a non-constrictive cuff was placed around the right femoral artery to induce accelerated atherosclerosis.					
29401645	0	43	theme	Accelerated	32:42	arg1	Atherosclerosis					44:58	Accelerated Atherosclerosis	32:58	Accelerated Atherosclerosis in Hypercholesterolemic APOE*3-Leiden Mice	32:101	The Prebiotic Inulin Aggravates Accelerated Atherosclerosis in Hypercholesterolemic APOE*3-Leiden Mice.					
29401645	5	44	theme	lesion	679:684	arg1	thickness					686:694	lesion thickness	679:694	lesion thickness	679:694	At week 5, vascular pathology was determined by lesion thickness, vascular remodeling, and lesion composition.					
29401645	12	45	theme	atherosclerosis	1560:1574	arg1	development					1576:1586	the aggravated atherosclerosis development	1545:1586	the aggravated atherosclerosis development	1545:1586	This process was not accompanied by differences in blood monocyte composition, suggesting that the aggravated atherosclerosis development was driven by increased plasma cholesterol.					
29401645	3	46	dep	high-cholesterol	433:448	arg1	%					452:452	1%	451:452	1%	451:452	Male E3L mice were fed a high-cholesterol (1%) diet, supplemented with or without 10% inulin for 5 weeks.					
29401645	7	47	theme	lesion	984:989	arg1	formation					991:999	increased lesion formation	974:999	increased lesion formation	974:999	In contrast to our hypothesis, inulin exacerbated atherosclerosis development, characterized by increased lesion formation and outward vascular remodeling.					
29401645	0	48	theme	Hypercholesterolemic	63:82	arg1	Mice					98:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	The Prebiotic Inulin Aggravates Accelerated Atherosclerosis in Hypercholesterolemic APOE*3-Leiden Mice.					
29401645	7	49	theme	vascular	1013:1020	arg1	remodeling					1022:1031	outward vascular remodeling	1005:1031	outward vascular remodeling	1005:1031	In contrast to our hypothesis, inulin exacerbated atherosclerosis development, characterized by increased lesion formation and outward vascular remodeling.					
29401645	12	50	from	differences	1486:1496	arg1	composition					1516:1526	blood monocyte composition	1501:1526	blood monocyte composition	1501:1526	This process was not accompanied by differences in blood monocyte composition, suggesting that the aggravated atherosclerosis development was driven by increased plasma cholesterol.					
29401645	8	51	theme	cells	1100:1104	arg1	number					1063:1068	increased number	1053:1068	increased number of macrophages, smooth muscle cells, and collagen content	1053:1126	The lesions showed increased number of macrophages, smooth muscle cells, and collagen content.					
29401645	2	52	theme	APOE	382:385	arg1	mice					402:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	12	53	theme	monocyte	1507:1514	arg1	composition					1516:1526	blood monocyte composition	1501:1526	blood monocyte composition	1501:1526	This process was not accompanied by differences in blood monocyte composition, suggesting that the aggravated atherosclerosis development was driven by increased plasma cholesterol.					
29401645	3	54	theme	%	492:492	arg1	inulin					494:499	10% inulin	490:499	10% inulin	490:499	Male E3L mice were fed a high-cholesterol (1%) diet, supplemented with or without 10% inulin for 5 weeks.					
29401645	2	55	theme	hypercholesterolemic	361:380	arg1	mice					402:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	10	56	theme	cholesterol	1226:1236	arg1	levels					1238:1243	plasma total cholesterol levels	1213:1243	plasma total cholesterol levels	1213:1243	Inulin significantly increased plasma total cholesterol levels and total cholesterol exposure.					
29401645	5	57	theme	vascular	642:649	arg1	pathology					651:659	vascular pathology	642:659	vascular pathology	642:659	At week 5, vascular pathology was determined by lesion thickness, vascular remodeling, and lesion composition.					
29401645	12	58	theme	blood	1501:1505	arg1	composition					1516:1526	blood monocyte composition	1501:1526	blood monocyte composition	1501:1526	This process was not accompanied by differences in blood monocyte composition, suggesting that the aggravated atherosclerosis development was driven by increased plasma cholesterol.					
29401645	7	59	theme	increased	974:982	arg1	formation					991:999	increased lesion formation	974:999	increased lesion formation	974:999	In contrast to our hypothesis, inulin exacerbated atherosclerosis development, characterized by increased lesion formation and outward vascular remodeling.					
29401645	11	60	theme	adverse	1399:1405	arg1	composition					1414:1424	adverse lesion composition	1399:1424	adverse lesion composition	1399:1424	In conclusion, inulin aggravated accelerated atherosclerosis development in hypercholesterolemic E3L mice, accompanied by adverse lesion composition and outward remodeling.					
29401645	1	61	theme	prebiotic	108:116	arg1	inulin					118:123	The prebiotic inulin	104:123	The prebiotic inulin	104:123	The prebiotic inulin has proven effective at lowering inflammation and plasma lipid levels.					
29401645	12	62	theme	increased	1602:1610	arg1	cholesterol					1619:1629	increased plasma cholesterol	1602:1629	increased plasma cholesterol	1602:1629	This process was not accompanied by differences in blood monocyte composition, suggesting that the aggravated atherosclerosis development was driven by increased plasma cholesterol.					
29401645	7	63	theme	outward	1005:1011	arg1	remodeling					1022:1031	outward vascular remodeling	1005:1031	outward vascular remodeling	1005:1031	In contrast to our hypothesis, inulin exacerbated atherosclerosis development, characterized by increased lesion formation and outward vascular remodeling.					
29401645	10	64	theme	plasma	1213:1218	arg1	levels					1238:1243	plasma total cholesterol levels	1213:1243	plasma total cholesterol levels	1213:1243	Inulin significantly increased plasma total cholesterol levels and total cholesterol exposure.					
29401645	0	65	theme	*	88:88	arg1	Mice					98:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	The Prebiotic Inulin Aggravates Accelerated Atherosclerosis in Hypercholesterolemic APOE*3-Leiden Mice.					
29401645	2	66	theme	E3L	397:399	arg1	mice					402:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	hypercholesterolemic APOE*3-Leiden (E3L) mice	361:405	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	2	67	from	effect	294:299	arg1	development					346:356	atherosclerosis development	330:356	atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice	330:405	As atherosclerosis is provoked by both inflammation and hyperlipidemia, we aimed to determine the effect of inulin supplementation on atherosclerosis development in hypercholesterolemic APOE*3-Leiden (E3L) mice.					
29401645	4	68	theme	femoral	577:583	arg1	artery					585:590	the right femoral artery	567:590	the right femoral artery	567:590	At week 3, a non-constrictive cuff was placed around the right femoral artery to induce accelerated atherosclerosis.					
29401645	0	69	theme	APOE	84:87	arg1	Mice					98:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	Hypercholesterolemic APOE*3-Leiden Mice	63:101	The Prebiotic Inulin Aggravates Accelerated Atherosclerosis in Hypercholesterolemic APOE*3-Leiden Mice.					
29401645	12	70	theme	plasma	1612:1617	arg1	cholesterol					1619:1629	increased plasma cholesterol	1602:1629	increased plasma cholesterol	1602:1629	This process was not accompanied by differences in blood monocyte composition, suggesting that the aggravated atherosclerosis development was driven by increased plasma cholesterol.					
29401645	7	71	theme	atherosclerosis	928:942	arg1	development					944:954	atherosclerosis development	928:954	atherosclerosis development	928:954	In contrast to our hypothesis, inulin exacerbated atherosclerosis development, characterized by increased lesion formation and outward vascular remodeling.					
29401645	10	72	theme	cholesterol	1255:1265	arg1	exposure					1267:1274	total cholesterol exposure	1249:1274	total cholesterol exposure	1249:1274	Inulin significantly increased plasma total cholesterol levels and total cholesterol exposure.					
29401645	4	73	theme	right	571:575	arg1	artery					585:590	the right femoral artery	567:590	the right femoral artery	567:590	At week 3, a non-constrictive cuff was placed around the right femoral artery to induce accelerated atherosclerosis.					
31473438	9	0	theme	structural	1640:1649	arg1	changes					1651:1657	the compositional and structural changes	1618:1657	the compositional and structural changes of collagen and elastin fibers	1618:1688	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	3	1	theme	dominant	685:692	arg1	role					694:697	a dominant role	683:697	a dominant role	683:697	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	3	2	from	concentration	503:515	arg1	disc					546:549	the disc	542:549	the disc	542:549	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	7	3	theme	collagenase	1429:1439	arg1	discs					1462:1466	collagenase and elastase treated discs	1429:1466	collagenase and elastase treated discs	1429:1466	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	4	theme	tangent	1417:1423	arg1	increase					1396:1403	increase	1396:1403	increase	1396:1403	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	4	theme	tangent	1417:1423	arg1	decrease					1383:1390	decrease	1383:1390	decrease	1383:1390	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	5	theme	treated	1454:1460	arg1	discs					1462:1466	collagenase and elastase treated discs	1429:1466	collagenase and elastase treated discs	1429:1466	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	6	from	proportion	1282:1291	arg1	loss					1319:1322	loss	1319:1322	loss	1319:1322	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	6	from	proportion	1282:1291	arg1	storage					1307:1313	storage	1307:1313	storage	1307:1313	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	4	7	dep	collagen	793:800	arg1	fibers					814:819	fibers	814:819	fibers	814:819	This study attempts to quantify the contribution of collagen and elastin fibers to the viscoelastic properties of the disc.					
31473438	7	8	from	changes	1296:1302	arg1	loss					1319:1322	loss	1319:1322	loss	1319:1322	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	8	from	changes	1296:1302	arg1	storage					1307:1313	storage	1307:1313	storage	1307:1313	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	6	9	theme	regions	1161:1167	arg1	moduli					1145:1150	the storage and loss moduli	1124:1150	moduli	1145:1150	Following both treatments, the storage and loss moduli of these regions were reduced dramatically up to the point that the tissue was no longer mechanically heterogeneous.					
31473438	0	10	theme	elastin	109:115	arg1	fibers					117:122	collagen and elastin fibers	96:122	collagen and elastin fibers from a perspective of polymer dynamics	96:161	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	5	11	theme	disc	1091:1094	arg1	regions					1076:1082	five different regions	1061:1082	five different regions of the disc	1061:1094	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	5	12	theme	different	1066:1074	arg1	regions					1076:1082	five different regions	1061:1082	five different regions of the disc	1061:1094	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	0	13	theme	polymer	146:152	arg1	dynamics					154:161	polymer dynamics	146:161	polymer dynamics	146:161	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	5	14	theme	fibrillar	935:943	arg1	network					945:951	the collagen and elastin fibrillar network	910:951	the collagen and elastin fibrillar network in porcine temporomandibular joint discs	910:992	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	9	15	theme	polymer	1752:1758	arg1	dynamics					1760:1767	polymer dynamics	1752:1767	polymer dynamics	1752:1767	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	0	16	from	role	88:91	arg1	perspective					131:141	a perspective	129:141	a perspective of polymer dynamics	129:161	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	0	17	theme	joint	72:76	arg1	disc					78:81	the temporomandibular joint disc	50:81	the temporomandibular joint disc	50:81	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	3	18	dep	permeability	482:493	arg1	the					473:475	the	473:475	the	473:475	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	9	19	with	consistent	1736:1745	arg1	dynamics					1760:1767	polymer dynamics	1752:1767	polymer dynamics	1752:1767	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	0	20	theme	collagen	96:103	arg1	fibers					117:122	collagen and elastin fibers	96:122	collagen and elastin fibers from a perspective of polymer dynamics	96:161	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	0	21	dep	role	88:91	arg1	properties					36:45	The dynamic mechanical viscoelastic properties	0:45	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc	0:81	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	2	22	theme	biopolymers	384:394	arg1	contribution					361:372	the contribution	357:372	the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc	357:443	Despite a large body of characterization studies, the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc is poorly understood.					
31473438	9	23	dep	collagen	1662:1669	arg1	fibers					1683:1688	fibers	1683:1688	fibers	1683:1688	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	2	24	theme	disc	440:443	arg1	behavior					424:431	the dynamic viscoelastic behavior	399:431	the dynamic viscoelastic behavior of the disc	399:443	Despite a large body of characterization studies, the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc is poorly understood.					
31473438	0	25	theme	mechanical	12:21	arg1	properties					36:45	The dynamic mechanical viscoelastic properties	0:45	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc	0:81	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	1	26	theme	macromolecular	279:292	arg1	biopolymers					294:304	macromolecular biopolymers	279:304	macromolecular biopolymers	279:304	The temporomandibular joint disc is a structure, characterized as heterogeneous fibrocartilage, and is composed of macromolecular biopolymers.					
31473438	5	27	theme	temporomandibular	964:980	arg1	discs					988:992	porcine temporomandibular joint discs	956:992	porcine temporomandibular joint discs	956:992	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	2	28	theme	viscoelastic	411:422	arg1	behavior					424:431	the dynamic viscoelastic behavior	399:431	the dynamic viscoelastic behavior of the disc	399:443	Despite a large body of characterization studies, the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc is poorly understood.					
31473438	5	29	theme	properties	1047:1056	arg1	changes					1015:1021	the changes	1011:1021	the changes of dynamic viscoelastic properties in five different regions of the disc	1011:1094	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	3	30	theme	viscoelastic	641:652	arg1	nature					654:659	the intrinsic viscoelastic nature	627:659	the intrinsic viscoelastic nature of solid matrix	627:675	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	2	31	theme	studies	348:354	arg1	body					323:326	a large body	315:326	a large body of characterization studies	315:354	Despite a large body of characterization studies, the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc is poorly understood.					
31473438	7	32	theme	changes	1296:1302	arg1	proportion					1282:1291	the proportion	1278:1291	the proportion of changes in storage and loss moduli	1278:1329	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	32	theme	changes	1296:1302	arg1	reflected					1366:1374	reflected	1366:1374	reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively	1366:1480	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	32	theme	changes	1296:1302	arg1	different					1336:1344	different	1336:1344	different	1336:1344	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	8	33	theme	length	1577:1582	arg1	decrease					1554:1561	a decrease	1552:1561	a decrease of biopolymer length	1552:1582	The reduction of storage and loss moduli of the disc correlated with a decrease of biopolymer length.					
31473438	9	34	theme	present	1589:1595	arg1	study					1597:1601	The present study	1585:1601	The present study	1585:1601	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	3	35	theme	high	477:480	arg1	permeability					482:493	high permeability	477:493	high permeability	477:493	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	7	36	dep	storage	1307:1313	arg1	moduli					1324:1329	moduli	1324:1329	moduli	1324:1329	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	3	37	theme	matrix	670:675	arg1	nature					654:659	the intrinsic viscoelastic nature	627:659	the intrinsic viscoelastic nature of solid matrix	627:675	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	3	38	theme	glycosaminoglycans	520:537	arg1	permeability					482:493	high permeability	477:493	high permeability	477:493	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	3	38	theme	glycosaminoglycans	520:537	arg1	concentration					503:515	low concentration	499:515	low concentration	499:515	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	5	39	theme	dynamic	1026:1032	arg1	properties					1047:1056	dynamic viscoelastic properties	1026:1056	dynamic viscoelastic properties	1026:1056	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	8	40	dep	storage	1500:1506	arg1	moduli					1517:1522	moduli	1517:1522	moduli	1517:1522	The reduction of storage and loss moduli of the disc correlated with a decrease of biopolymer length.					
31473438	9	41	theme	collagen	1662:1669	arg1	changes					1651:1657	the compositional and structural changes	1618:1657	the compositional and structural changes of collagen and elastin fibers	1618:1688	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	0	42	from	perspective	131:141	arg1	fibers					117:122	collagen and elastin fibers	96:122	collagen and elastin fibers from a perspective of polymer dynamics	96:161	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	0	42	from	perspective	131:141	arg1	role					88:91	The role	84:91	The role of collagen and elastin fibers from a perspective of polymer dynamics	84:161	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	1	43	theme	temporomandibular	168:184	arg1	disc					192:195	The temporomandibular joint disc	164:195	The temporomandibular joint disc	164:195	The temporomandibular joint disc is a structure, characterized as heterogeneous fibrocartilage, and is composed of macromolecular biopolymers.					
31473438	1	43	theme	temporomandibular	168:184	arg1	structure					202:210	a structure	200:210	a structure	200:210	The temporomandibular joint disc is a structure, characterized as heterogeneous fibrocartilage, and is composed of macromolecular biopolymers.					
31473438	7	44	theme	elastase	1445:1452	arg1	discs					1462:1466	collagenase and elastase treated discs	1429:1466	collagenase and elastase treated discs	1429:1466	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	9	45	theme	elastin	1675:1681	arg1	changes					1651:1657	the compositional and structural changes	1618:1657	the compositional and structural changes of collagen and elastin fibers	1618:1688	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	3	46	theme	time-dependent	716:729	arg1	behavior					731:738	its time-dependent behavior	712:738	its time-dependent behavior	712:738	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	3	47	from	permeability	482:493	arg1	disc					546:549	the disc	542:549	the disc	542:549	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	2	48	theme	large	317:321	arg1	body					323:326	a large body	315:326	a large body of characterization studies	315:354	Despite a large body of characterization studies, the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc is poorly understood.					
31473438	5	49	from	changes	1015:1021	arg1	regions					1076:1082	five different regions	1061:1082	five different regions of the disc	1061:1094	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	7	50	dep	decrease	1383:1390	arg1	the					1379:1381	the	1379:1381	the	1379:1381	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	4	51	theme	disc	859:862	arg1	properties					841:850	the viscoelastic properties	824:850	the viscoelastic properties of the disc	824:862	This study attempts to quantify the contribution of collagen and elastin fibers to the viscoelastic properties of the disc.					
31473438	7	52	theme	loss	1412:1415	arg1	tangent					1417:1423	the loss tangent	1408:1423	the loss tangent for collagenase and elastase treated discs	1408:1466	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	0	53	theme	fibers	117:122	arg1	role					88:91	The role	84:91	The role of collagen and elastin fibers from a perspective of polymer dynamics	84:161	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	9	54	theme	viscoelastic	1700:1711	arg1	properties					1713:1722	the viscoelastic properties	1696:1722	the viscoelastic properties of the disc	1696:1734	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	2	55	theme	characterization	331:346	arg1	studies					348:354	characterization studies	331:354	characterization studies	331:354	Despite a large body of characterization studies, the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc is poorly understood.					
31473438	1	56	theme	joint	186:190	arg1	disc					192:195	The temporomandibular joint disc	164:195	The temporomandibular joint disc	164:195	The temporomandibular joint disc is a structure, characterized as heterogeneous fibrocartilage, and is composed of macromolecular biopolymers.					
31473438	1	56	theme	joint	186:190	arg1	structure					202:210	a structure	200:210	a structure	200:210	The temporomandibular joint disc is a structure, characterized as heterogeneous fibrocartilage, and is composed of macromolecular biopolymers.					
31473438	3	57	theme	poro-elastic	579:590	arg1	behavior					592:599	poro-elastic behavior	579:599	poro-elastic behavior	579:599	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	5	58	theme	collagen	914:921	arg1	network					945:951	the collagen and elastin fibrillar network	910:951	the collagen and elastin fibrillar network in porcine temporomandibular joint discs	910:992	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	9	59	theme	disc	1731:1734	arg1	properties					1713:1722	the viscoelastic properties	1696:1722	the viscoelastic properties of the disc	1696:1734	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	2	60	from	contribution	361:372	arg1	behavior					424:431	the dynamic viscoelastic behavior	399:431	the dynamic viscoelastic behavior of the disc	399:443	Despite a large body of characterization studies, the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc is poorly understood.					
31473438	0	61	theme	dynamics	154:161	arg1	perspective					131:141	a perspective	129:141	a perspective of polymer dynamics	129:161	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	5	62	theme	elastin	927:933	arg1	network					945:951	the collagen and elastin fibrillar network	910:951	the collagen and elastin fibrillar network in porcine temporomandibular joint discs	910:992	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	0	63	theme	disc	78:81	arg1	properties					36:45	The dynamic mechanical viscoelastic properties	0:45	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc	0:81	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	8	64	theme	storage	1500:1506	arg1	reduction					1487:1495	The reduction	1483:1495	The reduction of storage and loss moduli of the disc	1483:1534	The reduction of storage and loss moduli of the disc correlated with a decrease of biopolymer length.					
31473438	8	65	theme	loss	1512:1515	arg1	reduction					1487:1495	The reduction	1483:1495	The reduction of storage and loss moduli of the disc	1483:1534	The reduction of storage and loss moduli of the disc correlated with a decrease of biopolymer length.					
31473438	5	66	theme	joint	982:986	arg1	discs					988:992	porcine temporomandibular joint discs	956:992	porcine temporomandibular joint discs	956:992	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	8	67	theme	disc	1531:1534	arg1	loss					1512:1515	loss	1512:1515	loss	1512:1515	The reduction of storage and loss moduli of the disc correlated with a decrease of biopolymer length.					
31473438	8	67	theme	disc	1531:1534	arg1	storage					1500:1506	storage	1500:1506	storage	1500:1506	The reduction of storage and loss moduli of the disc correlated with a decrease of biopolymer length.					
31473438	0	68	theme	dynamic	4:10	arg1	properties					36:45	The dynamic mechanical viscoelastic properties	0:45	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc	0:81	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	2	69	theme	matrix	377:382	arg1	biopolymers					384:394	matrix biopolymers	377:394	matrix biopolymers	377:394	Despite a large body of characterization studies, the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc is poorly understood.					
31473438	0	70	theme	viscoelastic	23:34	arg1	properties					36:45	The dynamic mechanical viscoelastic properties	0:45	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc	0:81	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	5	71	theme	porcine	956:962	arg1	discs					988:992	porcine temporomandibular joint discs	956:992	porcine temporomandibular joint discs	956:992	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	5	72	theme	viscoelastic	1034:1045	arg1	properties					1047:1056	dynamic viscoelastic properties	1026:1056	dynamic viscoelastic properties	1026:1056	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	8	73	theme	biopolymer	1566:1575	arg1	length					1577:1582	biopolymer length	1566:1582	biopolymer length	1566:1582	The reduction of storage and loss moduli of the disc correlated with a decrease of biopolymer length.					
31473438	3	74	theme	intrinsic	631:639	arg1	nature					654:659	the intrinsic viscoelastic nature	627:659	the intrinsic viscoelastic nature of solid matrix	627:675	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	2	75	theme	dynamic	403:409	arg1	behavior					424:431	the dynamic viscoelastic behavior	399:431	the dynamic viscoelastic behavior of the disc	399:443	Despite a large body of characterization studies, the contribution of matrix biopolymers on the dynamic viscoelastic behavior of the disc is poorly understood.					
31473438	0	76	theme	temporomandibular	54:70	arg1	disc					78:81	the temporomandibular joint disc	50:81	the temporomandibular joint disc	50:81	The dynamic mechanical viscoelastic properties of the temporomandibular joint disc: The role of collagen and elastin fibers from a perspective of polymer dynamics.					
31473438	7	77	dep	different	1336:1344	arg1	proportion					1282:1291	the proportion	1278:1291	the proportion of changes in storage and loss moduli	1278:1329	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	77	dep	different	1336:1344	arg1	reflected					1366:1374	reflected	1366:1374	reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively	1366:1480	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	77	dep	different	1336:1344	arg1	different					1336:1344	different	1336:1344	different	1336:1344	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	78	from	storage	1307:1313	arg1	proportion					1282:1291	the proportion	1278:1291	the proportion of changes in storage and loss moduli	1278:1329	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	78	from	storage	1307:1313	arg1	reflected					1366:1374	reflected	1366:1374	reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively	1366:1480	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	78	from	storage	1307:1313	arg1	different					1336:1344	different	1336:1344	different	1336:1344	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	4	79	theme	viscoelastic	828:839	arg1	properties					841:850	the viscoelastic properties	824:850	the viscoelastic properties of the disc	824:862	This study attempts to quantify the contribution of collagen and elastin fibers to the viscoelastic properties of the disc.					
31473438	3	80	theme	low	499:501	arg1	concentration					503:515	low concentration	499:515	low concentration	499:515	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	7	81	from	loss	1319:1322	arg1	proportion					1282:1291	the proportion	1278:1291	the proportion of changes in storage and loss moduli	1278:1329	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	81	from	loss	1319:1322	arg1	reflected					1366:1374	reflected	1366:1374	reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively	1366:1480	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	7	81	from	loss	1319:1322	arg1	different					1336:1344	different	1336:1344	different	1336:1344	However, the proportion of changes in storage and loss moduli were different for each treatment, reflected in the decrease and increase of the loss tangent for collagenase and elastase treated discs, respectively.					
31473438	6	82	theme	loss	1140:1143	arg1	moduli					1145:1150	the storage and loss moduli	1124:1150	moduli	1145:1150	Following both treatments, the storage and loss moduli of these regions were reduced dramatically up to the point that the tissue was no longer mechanically heterogeneous.					
31473438	3	83	theme	solid	664:668	arg1	matrix					670:675	solid matrix	664:675	solid matrix	664:675	Given the high permeability and low concentration of glycosaminoglycans in the disc, it has been suggested that poro-elastic behavior can be neglected and that the intrinsic viscoelastic nature of solid matrix plays a dominant role in governing its time-dependent behavior.					
31473438	4	84	theme	collagen	793:800	arg1	contribution					777:788	the contribution	773:788	the contribution of collagen and elastin fibers to the viscoelastic properties of the disc	773:862	This study attempts to quantify the contribution of collagen and elastin fibers to the viscoelastic properties of the disc.					
31473438	5	85	from	network	945:951	arg1	discs					988:992	porcine temporomandibular joint discs	956:992	porcine temporomandibular joint discs	956:992	Using collagenase and elastase, we perturbed the collagen and elastin fibrillar network in porcine temporomandibular joint discs and investigated the changes of dynamic viscoelastic properties in five different regions of the disc.					
31473438	9	86	theme	compositional	1622:1634	arg1	changes					1651:1657	the compositional and structural changes	1618:1657	the compositional and structural changes of collagen and elastin fibers	1618:1688	The present study indicates that the compositional and structural changes of collagen and elastin fibers alter the viscoelastic properties of the disc consistent with polymer dynamics.					
31473438	6	87	theme	storage	1128:1134	arg1	moduli					1145:1150	the storage and loss moduli	1124:1150	moduli	1145:1150	Following both treatments, the storage and loss moduli of these regions were reduced dramatically up to the point that the tissue was no longer mechanically heterogeneous.					
31473438	4	88	theme	elastin	806:812	arg1	contribution					777:788	the contribution	773:788	the contribution of collagen and elastin fibers to the viscoelastic properties of the disc	773:862	This study attempts to quantify the contribution of collagen and elastin fibers to the viscoelastic properties of the disc.					
31473438	1	89	theme	heterogeneous	230:242	arg1	fibrocartilage					244:257	heterogeneous fibrocartilage	230:257	heterogeneous fibrocartilage	230:257	The temporomandibular joint disc is a structure, characterized as heterogeneous fibrocartilage, and is composed of macromolecular biopolymers.					
30732760	3	0	dep	determined	239:248	arg1	glucose					306:312	glucose	306:312	glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5	306:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	7	1	theme	-positive	995:1003	arg1	%					1016:1016	37.0%	1012:1016	37.0%	1012:1016	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	7	1	theme	-positive	995:1003	arg1	cells					1005:1009	annexin V-positive and propidium iodide (PI)-positive cells	951:1009	annexin V-positive and propidium iodide (PI)-positive cells (37.0%)	951:1017	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	1	2	theme	plant	141:145	arg1	kingdom					147:153	the plant kingdom	137:153	the plant kingdom	137:153	Xylan is one of the most abundant hemicellulose constituents in the plant kingdom.					
30732760	4	3	theme	human	514:518	arg1	cells					551:555	human lung adenocarcinoma epithelial cells	514:555	human lung adenocarcinoma epithelial cells (A549)	514:562	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	4	3	theme	human	514:518	arg1	A549					558:561	A549	558:561	A549	558:561	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	6	4	theme	nuclear	878:884	arg1	condensation					886:897	nuclear condensation	878:897	nuclear condensation	878:897	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	6	4	theme	nuclear	878:884	arg1	bodies					870:875	condensed chromatin bodies	850:875	condensed chromatin bodies (nuclear condensation)	850:898	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	4	5	theme	cells	461:465	arg1	proliferation					426:438	the proliferation	422:438	the proliferation of murine macrophage cells (RAW 264.7)	422:477	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	7	6	theme	cells	1005:1009	arg1	number					941:946	the number	937:946	the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%)	937:1017	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	8	7	theme	Bcl-2	1135:1139	arg1	ratio					1122:1126	the ratio	1118:1126	the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells	1118:1243	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	5	8	theme	Several	626:632	arg1	changes					670:676	Several cell death-associated morphological changes	626:676	Several cell death-associated morphological changes	626:676	Several cell death-associated morphological changes were observed after the exposure of HeLa cells to XCC for 24 h.					
30732760	3	9	from	acid	353:356	arg1	ratio					380:384	a molar percentage ratio	361:384	a molar percentage ratio of 48:21:16:10:2.5:2.5	361:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	8	10	from	ratio	1122:1126	arg1	cells					1239:1243	treated cells	1231:1243	treated cells	1231:1243	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	4	11	theme	adenocarcinoma	525:538	arg1	cells					551:555	human lung adenocarcinoma epithelial cells	514:555	human lung adenocarcinoma epithelial cells (A549)	514:562	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	4	11	theme	adenocarcinoma	525:538	arg1	A549					558:561	A549	558:561	A549	558:561	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	4	12	theme	lung	520:523	arg1	cells					551:555	human lung adenocarcinoma epithelial cells	514:555	human lung adenocarcinoma epithelial cells (A549)	514:562	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	4	12	theme	lung	520:523	arg1	A549					558:561	A549	558:561	A549	558:561	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	3	13	theme	xylose	299:304	arg1	composition					284:294	a composition	282:294	a composition of xylose	282:304	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	3	14	theme	molar	363:367	arg1	ratio					380:384	a molar percentage ratio	361:384	a molar percentage ratio of 48:21:16:10:2.5:2.5	361:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	5	15	theme	HeLa	714:717	arg1	cells					719:723	HeLa cells	714:723	HeLa cells	714:723	Several cell death-associated morphological changes were observed after the exposure of HeLa cells to XCC for 24 h.					
30732760	4	16	dep	inhibited	483:491	arg1	HeLa					612:615	HeLa	612:615	HeLa	612:615	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	7	17	from	increase	925:932	arg1	number					941:946	the number	937:946	the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%)	937:1017	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	3	18	theme	percentage	369:378	arg1	ratio					380:384	a molar percentage ratio	361:384	a molar percentage ratio of 48:21:16:10:2.5:2.5	361:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	5	19	theme	cells	719:723	arg1	exposure					702:709	the exposure	698:709	the exposure of HeLa cells to XCC for 24 h	698:739	Several cell death-associated morphological changes were observed after the exposure of HeLa cells to XCC for 24 h.					
30732760	0	20	from	cobs	34:37	arg1	xylan					18:22	Antiproliferative xylan	0:22	Antiproliferative xylan from corn cobs	0:37	Antiproliferative xylan from corn cobs induces apoptosis in tumor cells.					
30732760	1	21	from	constituents	121:132	arg1	kingdom					147:153	the plant kingdom	137:153	the plant kingdom	137:153	Xylan is one of the most abundant hemicellulose constituents in the plant kingdom.					
30732760	4	22	theme	adenocarcinoma	590:603	arg1	cells					605:609	human cervical adenocarcinoma cells	575:609	human cervical adenocarcinoma cells	575:609	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	0	23	theme	Antiproliferative	0:16	arg1	xylan					18:22	Antiproliferative xylan	0:22	Antiproliferative xylan from corn cobs	0:37	Antiproliferative xylan from corn cobs induces apoptosis in tumor cells.					
30732760	7	24	theme	propidium	974:982	arg1	iodide					984:989	propidium iodide	974:989	propidium iodide	974:989	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	9	25	theme	HeLa	1349:1352	arg1	cells					1354:1358	HeLa cells	1349:1358	HeLa cells	1349:1358	Therefore, the results demonstrated the antiproliferative potential of XCC, which induced apoptosis in HeLa cells.					
30732760	4	26	theme	cervical	581:588	arg1	cells					605:609	human cervical adenocarcinoma cells	575:609	human cervical adenocarcinoma cells	575:609	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	0	27	theme	corn	29:32	arg1	cobs					34:37	corn cobs	29:37	corn cobs	29:37	Antiproliferative xylan from corn cobs induces apoptosis in tumor cells.					
30732760	6	28	theme	chromatin	860:868	arg1	condensation					886:897	nuclear condensation	878:897	nuclear condensation	878:897	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	6	28	theme	chromatin	860:868	arg1	bodies					870:875	condensed chromatin bodies	850:875	condensed chromatin bodies (nuclear condensation)	850:898	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	6	29	with	nuclei	838:843	arg1	condensation					886:897	nuclear condensation	878:897	nuclear condensation	878:897	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	6	29	with	nuclei	838:843	arg1	bodies					870:875	condensed chromatin bodies	850:875	condensed chromatin bodies (nuclear condensation)	850:898	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	3	30	theme	48:21:16:10:2.5:2.5	389:407	arg1	ratio					380:384	a molar percentage ratio	361:384	a molar percentage ratio of 48:21:16:10:2.5:2.5	361:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	6	31	theme	condensed	850:858	arg1	condensation					886:897	nuclear condensation	878:897	nuclear condensation	878:897	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	6	31	theme	condensed	850:858	arg1	bodies					870:875	condensed chromatin bodies	850:875	condensed chromatin bodies (nuclear condensation)	850:898	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	7	32	theme	V-positive	959:968	arg1	%					1016:1016	37.0%	1012:1016	37.0%	1012:1016	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	7	32	theme	V-positive	959:968	arg1	cells					1005:1009	annexin V-positive and propidium iodide (PI)-positive cells	951:1009	annexin V-positive and propidium iodide (PI)-positive cells (37.0%)	951:1017	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	6	33	theme	fluorescent	764:774	arg1	probes					776:781	fluorescent probes	764:781	fluorescent probes	764:781	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	8	34	theme	cytochrome	1167:1176	arg1	c					1178:1178	cytochrome c	1167:1178	cytochrome c	1167:1178	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	9	35	theme	XCC	1317:1319	arg1	potential					1304:1312	the antiproliferative potential	1282:1312	the antiproliferative potential	1282:1312	Therefore, the results demonstrated the antiproliferative potential of XCC, which induced apoptosis in HeLa cells.					
30732760	3	36	theme	glucuronic	342:351	arg1	acid					353:356	glucuronic acid	342:356	glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5	306:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	3	37	theme	XCC	231:233	arg1	structure					218:226	The structure	214:226	The structure of XCC	214:233	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	7	38	theme	XCC	1038:1040	arg1	presence					1026:1033	the presence	1022:1033	the presence of XCC	1022:1040	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	8	39	theme	caspase	1181:1187	arg1	ratio					1122:1126	the ratio	1118:1126	the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells	1118:1243	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	4	40	theme	human	575:579	arg1	cells					605:609	human cervical adenocarcinoma cells	575:609	human cervical adenocarcinoma cells	575:609	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	6	41	theme	nuclei	838:843	arg1	number					805:810	a larger number	796:810	a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation)	796:898	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	8	42	theme	treated	1231:1237	arg1	cells					1239:1243	treated cells	1231:1243	treated cells	1231:1243	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	7	43	dep	-positive	995:1003	arg1	iodide					984:989	propidium iodide	974:989	propidium iodide	974:989	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	5	44	theme	morphological	656:668	arg1	changes					670:676	Several cell death-associated morphological changes	626:676	Several cell death-associated morphological changes	626:676	Several cell death-associated morphological changes were observed after the exposure of HeLa cells to XCC for 24 h.					
30732760	6	45	theme	pyknotic	829:836	arg1	nuclei					838:843	irregular and pyknotic nuclei	815:843	irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation)	815:898	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	7	46	theme	control	1077:1083	arg1	cells					1085:1089	the negative control cells	1064:1089	the negative control cells (5.0%)	1064:1096	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	7	46	theme	control	1077:1083	arg1	%					1095:1095	5.0%	1092:1095	5.0%	1092:1095	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	3	47	dep	glucose	306:312	arg1	galactose					324:332	galactose	324:332	glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5	306:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	3	47	dep	glucose	306:312	arg1	arabinose					314:322	arabinose	314:322	glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5	306:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	3	47	dep	glucose	306:312	arg1	mannose					334:340	mannose	334:340	glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5	306:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	3	47	dep	glucose	306:312	arg1	acid					353:356	glucuronic acid	342:356	glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5	306:407	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	4	48	theme	macrophage	450:459	arg1	RAW					468:470	RAW 264.7	468:476	RAW 264.7	468:476	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	4	48	theme	macrophage	450:459	arg1	cells					461:465	murine macrophage cells	443:465	murine macrophage cells (RAW 264.7)	443:477	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	6	49	theme	larger	798:803	arg1	number					805:810	a larger number	796:810	a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation)	796:898	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	1	50	theme	abundant	98:105	arg1	constituents					121:132	the most abundant hemicellulose constituents	89:132	the most abundant hemicellulose constituents in the plant kingdom	89:153	Xylan is one of the most abundant hemicellulose constituents in the plant kingdom.					
30732760	6	51	theme	irregular	815:823	arg1	nuclei					838:843	irregular and pyknotic nuclei	815:843	irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation)	815:898	In addition, by using fluorescent probes, we observed a larger number of irregular and pyknotic nuclei with condensed chromatin bodies (nuclear condensation).					
30732760	3	52	theme	NMR	253:255	arg1	analysis					257:264	NMR analysis	253:264	NMR analysis	253:264	The structure of XCC was determined by NMR analysis, which revealed a composition of xylose:glucose:arabinose:galactose:mannose:glucuronic acid in a molar percentage ratio of 48:21:16:10:2.5:2.5.					
30732760	8	53	theme	apoptosis-inducing	1196:1213	arg1	AIF					1223:1225	AIF	1223:1225	AIF	1223:1225	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	8	53	theme	apoptosis-inducing	1196:1213	arg1	factor					1215:1220	apoptosis-inducing factor	1196:1220	apoptosis-inducing factor (AIF)	1196:1226	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	7	54	theme	FACS	901:904	arg1	analysis					906:913	FACS analysis	901:913	FACS analysis	901:913	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	1	55	theme	hemicellulose	107:119	arg1	constituents					121:132	the most abundant hemicellulose constituents	89:132	the most abundant hemicellulose constituents in the plant kingdom	89:153	Xylan is one of the most abundant hemicellulose constituents in the plant kingdom.					
30732760	8	56	theme	c	1178:1178	arg1	AIF					1223:1225	AIF	1223:1225	AIF	1223:1225	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	8	56	theme	c	1178:1178	arg1	Bcl-2					1135:1139	Bcl-2	1135:1139	Bcl-2 (3.5:1)	1135:1147	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	8	56	theme	c	1178:1178	arg1	levels					1157:1162	the levels	1153:1162	the levels of cytochrome c	1153:1178	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	8	56	theme	c	1178:1178	arg1	3.5:1					1142:1146	3.5:1	1142:1146	3.5:1	1142:1146	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	8	56	theme	c	1178:1178	arg1	factor					1215:1220	apoptosis-inducing factor	1196:1220	apoptosis-inducing factor (AIF)	1196:1226	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	8	56	theme	c	1178:1178	arg1	caspase					1181:1187	caspase 3	1181:1189	caspase 3	1181:1189	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	8	56	theme	c	1178:1178	arg1	Bax					1131:1133	Bax	1131:1133	Bax	1131:1133	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	4	57	theme	cells	551:555	arg1	proliferation					497:509	the proliferation	493:509	the proliferation of human lung adenocarcinoma epithelial cells (A549)	493:562	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	2	58	theme	corn	180:183	arg1	XCC					191:193	XCC	191:193	XCC	191:193	We extracted xylan from corn cobs (XCC) using ultrasound.					
30732760	2	58	theme	corn	180:183	arg1	cobs					185:188	corn cobs	180:188	corn cobs (XCC)	180:194	We extracted xylan from corn cobs (XCC) using ultrasound.					
30732760	1	59	theme	constituents	121:132	arg1	one					82:84	one	82:84	one	82:84	Xylan is one of the most abundant hemicellulose constituents in the plant kingdom.					
30732760	1	59	theme	constituents	121:132	arg1	constituents					121:132	the most abundant hemicellulose constituents	89:132	the most abundant hemicellulose constituents in the plant kingdom	89:153	Xylan is one of the most abundant hemicellulose constituents in the plant kingdom.					
30732760	4	60	theme	epithelial	540:549	arg1	cells					551:555	human lung adenocarcinoma epithelial cells	514:555	human lung adenocarcinoma epithelial cells (A549)	514:562	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	4	60	theme	epithelial	540:549	arg1	A549					558:561	A549	558:561	A549	558:561	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	7	61	theme	negative	1068:1075	arg1	cells					1085:1089	the negative control cells	1064:1089	the negative control cells (5.0%)	1064:1096	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	7	61	theme	negative	1068:1075	arg1	%					1095:1095	5.0%	1092:1095	5.0%	1092:1095	FACS analysis showed an increase in the number of annexin V-positive and propidium iodide (PI)-positive cells (37.0%) in the presence of XCC compared with that of the negative control cells (5.0%).					
30732760	9	62	theme	antiproliferative	1286:1302	arg1	potential					1304:1312	the antiproliferative potential	1282:1312	the antiproliferative potential	1282:1312	Therefore, the results demonstrated the antiproliferative potential of XCC, which induced apoptosis in HeLa cells.					
30732760	0	63	theme	tumor	60:64	arg1	cells					66:70	tumor cells	60:70	tumor cells	60:70	Antiproliferative xylan from corn cobs induces apoptosis in tumor cells.					
30732760	4	64	theme	murine	443:448	arg1	RAW					468:470	RAW 264.7	468:476	RAW 264.7	468:476	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	4	64	theme	murine	443:448	arg1	cells					461:465	murine macrophage cells	443:465	murine macrophage cells (RAW 264.7)	443:477	XCC induced the proliferation of murine macrophage cells (RAW 264.7) and inhibited the proliferation of human lung adenocarcinoma epithelial cells (A549) by 20% and human cervical adenocarcinoma cells (HeLa) by 60%.					
30732760	8	65	theme	factor	1215:1220	arg1	ratio					1122:1126	the ratio	1118:1126	the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells	1118:1243	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	8	66	theme	Bax	1131:1133	arg1	ratio					1122:1126	the ratio	1118:1126	the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells	1118:1243	XCC also increased the ratio of Bax:Bcl-2 (3.5:1) and the levels of cytochrome c, caspase 3, and apoptosis-inducing factor (AIF) in treated cells.					
30732760	5	67	theme	death-associated	639:654	arg1	changes					670:676	Several cell death-associated morphological changes	626:676	Several cell death-associated morphological changes	626:676	Several cell death-associated morphological changes were observed after the exposure of HeLa cells to XCC for 24 h.					
31552691	0	0	theme	three-dimensional	89:105	arg1	environment					107:117	a three-dimensional environment	87:117	a three-dimensional environment	87:117	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	7	1	theme	confocal	976:983	arg1	microscopy					985:994	confocal microscopy	976:994	confocal microscopy	976:994	After 3, 14, and 28 days, the constructs were analyzed with quantitative polymerase chain reaction and confocal microscopy and sulfated glycosaminoglycans (GAGs) were measured.					
31552691	10	2	theme	highest	1253:1259	arg1	levels					1272:1277	the highest expression levels	1249:1277	the highest expression levels of hypertrophic genes	1249:1299	The CG membrane induced the highest expression levels of hypertrophic genes.					
31552691	0	3	from	differentiation	13:27	arg1	environment					107:117	a three-dimensional environment	87:117	a three-dimensional environment	87:117	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	7	4	theme	chain	957:961	arg1	reaction					963:970	quantitative polymerase chain reaction	933:970	quantitative polymerase chain reaction	933:970	After 3, 14, and 28 days, the constructs were analyzed with quantitative polymerase chain reaction and confocal microscopy and sulfated glycosaminoglycans (GAGs) were measured.					
31552691	13	5	theme	cell	1535:1538	arg1	fate					1540:1543	cell fate	1535:1543	cell fate	1535:1543	Thus, caution for cell fate is required when designing cell-biomaterial constructs for cartilage regeneration.					
31552691	12	6	theme	chondrocyte	1492:1502	arg1	hypertrophy					1504:1514	chondrocyte hypertrophy	1492:1514	chondrocyte hypertrophy	1492:1514	Regardless of the biomaterial scaffold, culturing BM-MSCs in chondrogenic differentiation medium resulted in chondrocyte hypertrophy.					
31552691	7	7	theme	quantitative	933:944	arg1	reaction					963:970	quantitative polymerase chain reaction	933:970	quantitative polymerase chain reaction	933:970	After 3, 14, and 28 days, the constructs were analyzed with quantitative polymerase chain reaction and confocal microscopy and sulfated glycosaminoglycans (GAGs) were measured.					
31552691	4	8	theme	commercial	685:694	arg1	I/III					710:714	commercial collagen type I/III	685:714	commercial collagen type I/III membrane (CG)	685:728	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	3	9	theme	chondrogenic	464:475	arg1	capability					477:486	The chondrogenic capability	460:486	The chondrogenic capability of human BM-MSCs	460:503	The chondrogenic capability of human BM-MSCs was first verified in a pellet culture.					
31552691	12	10	theme	biomaterial	1401:1411	arg1	scaffold					1413:1420	the biomaterial scaffold	1397:1420	the biomaterial scaffold	1397:1420	Regardless of the biomaterial scaffold, culturing BM-MSCs in chondrogenic differentiation medium resulted in chondrocyte hypertrophy.					
31552691	6	11	theme	human	828:832	arg1	ACs					848:850	ACs	848:850	ACs	848:850	Adult human chondrocytes (ACs) served as controls.					
31552691	6	11	theme	human	828:832	arg1	chondrocytes					834:845	Adult human chondrocytes	822:845	Adult human chondrocytes (ACs)	822:851	Adult human chondrocytes (ACs) served as controls.					
31552691	6	11	theme	human	828:832	arg1	controls					863:870	controls	863:870	controls	863:870	Adult human chondrocytes (ACs) served as controls.					
31552691	2	12	theme	biomaterial	329:339	arg1	scaffolds					341:349	three-dimensional biomaterial scaffolds	311:349	three-dimensional biomaterial scaffolds	311:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	10	13	theme	genes	1295:1299	arg1	levels					1272:1277	the highest expression levels	1249:1277	the highest expression levels of hypertrophic genes	1249:1299	The CG membrane induced the highest expression levels of hypertrophic genes.					
31552691	11	14	from	types	1329:1333	arg1	scaffolds					1348:1356	composite scaffolds	1338:1356	composite scaffolds	1338:1356	The two different collagen types in composite scaffolds yielded similar results.					
31552691	8	15	theme	differentiated	1054:1067	arg1	BM-MSCs					1069:1075	The differentiated BM-MSCs	1050:1075	The differentiated BM-MSCs	1050:1075	The differentiated BM-MSCs entered a hypertrophic state by Day 14 of culture.					
31552691	6	16	theme	Adult	822:826	arg1	ACs					848:850	ACs	848:850	ACs	848:850	Adult human chondrocytes (ACs) served as controls.					
31552691	6	16	theme	Adult	822:826	arg1	chondrocytes					834:845	Adult human chondrocytes	822:845	Adult human chondrocytes (ACs)	822:851	Adult human chondrocytes (ACs) served as controls.					
31552691	6	16	theme	Adult	822:826	arg1	controls					863:870	controls	863:870	controls	863:870	Adult human chondrocytes (ACs) served as controls.					
31552691	1	17	used	used	172:175	arg2	therapy					125:131	Cell therapy	120:131	Cell therapy combined with biomaterial scaffolds	120:167	Cell therapy combined with biomaterial scaffolds is used to treat cartilage defects.					
31552691	4	18	theme	type	705:708	arg1	I/III					710:714	commercial collagen type I/III	685:714	commercial collagen type I/III membrane (CG)	685:728	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	1	19	theme	cartilage	186:194	arg1	defects					196:202	cartilage defects	186:202	cartilage defects	186:202	Cell therapy combined with biomaterial scaffolds is used to treat cartilage defects.					
31552691	12	20	from	BM-MSCs	1433:1439	arg1	medium					1473:1478	chondrogenic differentiation medium	1444:1478	chondrogenic differentiation medium	1444:1478	Regardless of the biomaterial scaffold, culturing BM-MSCs in chondrogenic differentiation medium resulted in chondrocyte hypertrophy.					
31552691	12	21	theme	differentiation	1457:1471	arg1	medium					1473:1478	chondrogenic differentiation medium	1444:1478	chondrogenic differentiation medium	1444:1478	Regardless of the biomaterial scaffold, culturing BM-MSCs in chondrogenic differentiation medium resulted in chondrocyte hypertrophy.					
31552691	5	22	theme	chondrogenic	793:804	arg1	medium					814:819	chondrogenic culture medium	793:819	chondrogenic culture medium	793:819	The BM-MSCs were either cultured in a proliferation medium or chondrogenic culture medium.					
31552691	9	23	theme	low	1206:1208	arg1	genes					1196:1200	chondrogenic genes	1183:1200	chondrogenic genes	1183:1200	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	9	23	theme	low	1206:1208	arg1	amount					1210:1215	low amount	1206:1215	low amount of GAG	1206:1222	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	9	23	theme	low	1206:1208	arg1	GAG					1220:1222	GAG	1220:1222	GAG	1220:1222	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	11	24	theme	composite	1338:1346	arg1	scaffolds					1348:1356	composite scaffolds	1338:1356	composite scaffolds	1338:1356	The two different collagen types in composite scaffolds yielded similar results.					
31552691	12	25	theme	chondrogenic	1444:1455	arg1	differentiation					1457:1471	chondrogenic differentiation	1444:1471	chondrogenic differentiation medium	1444:1478	Regardless of the biomaterial scaffold, culturing BM-MSCs in chondrogenic differentiation medium resulted in chondrocyte hypertrophy.					
31552691	2	26	theme	matrix	380:385	arg1	deposition					387:396	cartilaginous matrix deposition	366:396	cartilaginous matrix deposition	366:396	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	3	27	theme	BM-MSCs	497:503	arg1	capability					477:486	The chondrogenic capability	460:486	The chondrogenic capability of human BM-MSCs	460:503	The chondrogenic capability of human BM-MSCs was first verified in a pellet culture.					
31552691	0	28	theme	Chondrogenic	0:11	arg1	differentiation					13:27	Chondrogenic differentiation	0:27	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.	0:118	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	0	29	link	marrow-derived	43:56	arg1	cells					78:82	human bone marrow-derived mesenchymal stromal cells	32:82	human bone marrow-derived mesenchymal stromal cells	32:82	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	2	30	theme	cartilaginous	366:378	arg1	deposition					387:396	cartilaginous matrix deposition	366:396	cartilaginous matrix deposition	366:396	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	4	31	theme	collagen	696:703	arg1	I/III					710:714	commercial collagen type I/III	685:714	commercial collagen type I/III membrane (CG)	685:728	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	0	32	theme	human	32:36	arg1	cells					78:82	human bone marrow-derived mesenchymal stromal cells	32:82	human bone marrow-derived mesenchymal stromal cells	32:82	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	8	33	theme	hypertrophic	1087:1098	arg1	state					1100:1104	a hypertrophic state	1085:1104	a hypertrophic state	1085:1104	The differentiated BM-MSCs entered a hypertrophic state by Day 14 of culture.					
31552691	7	34	theme	polymerase	946:955	arg1	reaction					963:970	quantitative polymerase chain reaction	933:970	quantitative polymerase chain reaction	933:970	After 3, 14, and 28 days, the constructs were analyzed with quantitative polymerase chain reaction and confocal microscopy and sulfated glycosaminoglycans (GAGs) were measured.					
31552691	1	35	theme	Cell	120:123	arg1	therapy					125:131	Cell therapy	120:131	Cell therapy combined with biomaterial scaffolds	120:167	Cell therapy combined with biomaterial scaffolds is used to treat cartilage defects.					
31552691	2	36	from	cells	292:296	arg1	scaffolds					341:349	three-dimensional biomaterial scaffolds	311:349	three-dimensional biomaterial scaffolds	311:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	3	37	theme	pellet	529:534	arg1	culture					536:542	a pellet culture	527:542	a pellet culture	527:542	The chondrogenic capability of human BM-MSCs was first verified in a pellet culture.					
31552691	4	38	theme	collagen	642:649	arg1	type					651:654	collagen type II	642:657	collagen type II (C2)	642:662	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	4	38	theme	collagen	642:649	arg1	C2					660:661	C2	660:661	C2	660:661	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	4	38	theme	collagen	642:649	arg1	C3					677:678	C3	677:678	C3	677:678	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	2	39	theme	three-dimensional	311:327	arg1	scaffolds					341:349	three-dimensional biomaterial scaffolds	311:349	three-dimensional biomaterial scaffolds	311:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	11	40	theme	collagen	1320:1327	arg1	types					1329:1333	The two different collagen types	1302:1333	The two different collagen types in composite scaffolds	1302:1356	The two different collagen types in composite scaffolds yielded similar results.					
31552691	12	41	theme	culturing	1423:1431	arg1	BM-MSCs					1433:1439	culturing BM-MSCs	1423:1439	culturing BM-MSCs in chondrogenic differentiation medium	1423:1478	Regardless of the biomaterial scaffold, culturing BM-MSCs in chondrogenic differentiation medium resulted in chondrocyte hypertrophy.					
31552691	0	42	theme	marrow-derived	43:56	arg1	cells					78:82	human bone marrow-derived mesenchymal stromal cells	32:82	human bone marrow-derived mesenchymal stromal cells	32:82	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	10	43	theme	hypertrophic	1282:1293	arg1	genes					1295:1299	hypertrophic genes	1282:1299	hypertrophic genes	1282:1299	The CG membrane induced the highest expression levels of hypertrophic genes.					
31552691	2	44	theme	stem	287:290	arg1	BM-MSCs					299:305	BM-MSCs	299:305	BM-MSCs	299:305	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	2	44	theme	stem	287:290	arg1	cells					292:296	bone marrow-derived mesenchymal stem cells	255:296	chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds	226:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	13	45	theme	cell-biomaterial	1572:1587	arg1	constructs					1589:1598	cell-biomaterial constructs	1572:1598	cell-biomaterial constructs	1572:1598	Thus, caution for cell fate is required when designing cell-biomaterial constructs for cartilage regeneration.					
31552691	0	46	theme	bone	38:41	arg1	cells					78:82	human bone marrow-derived mesenchymal stromal cells	32:82	human bone marrow-derived mesenchymal stromal cells	32:82	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	4	47	theme	composite	588:596	arg1	rhCo-PLA					607:614	a composite scaffold rhCo-PLA	586:614	a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG)	586:728	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	3	48	theme	human	491:495	arg1	BM-MSCs					497:503	human BM-MSCs	491:503	human BM-MSCs	491:503	The chondrogenic capability of human BM-MSCs was first verified in a pellet culture.					
31552691	2	49	theme	mesenchymal	275:285	arg1	BM-MSCs					299:305	BM-MSCs	299:305	BM-MSCs	299:305	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	2	49	theme	mesenchymal	275:285	arg1	cells					292:296	bone marrow-derived mesenchymal stem cells	255:296	chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds	226:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	0	50	theme	stromal	70:76	arg1	cells					78:82	human bone marrow-derived mesenchymal stromal cells	32:82	human bone marrow-derived mesenchymal stromal cells	32:82	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	9	51	theme	genes	1196:1200	arg1	genes					1196:1200	chondrogenic genes	1183:1200	chondrogenic genes	1183:1200	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	9	51	theme	genes	1196:1200	arg1	amount					1210:1215	low amount	1206:1215	low amount of GAG	1206:1222	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	9	51	theme	genes	1196:1200	arg1	GAG					1220:1222	GAG	1220:1222	GAG	1220:1222	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	9	51	theme	genes	1196:1200	arg1	expression					1169:1178	no expression	1166:1178	no expression of chondrogenic genes	1166:1200	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	5	52	theme	proliferation	769:781	arg1	medium					783:788	a proliferation medium	767:788	a proliferation medium	767:788	The BM-MSCs were either cultured in a proliferation medium or chondrogenic culture medium.					
31552691	2	53	theme	marrow-derived	260:273	arg1	BM-MSCs					299:305	BM-MSCs	299:305	BM-MSCs	299:305	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	2	53	theme	marrow-derived	260:273	arg1	cells					292:296	bone marrow-derived mesenchymal stem cells	255:296	chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds	226:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	9	54	theme	GAG	1220:1222	arg1	genes					1196:1200	chondrogenic genes	1183:1200	chondrogenic genes	1183:1200	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	9	54	theme	GAG	1220:1222	arg1	amount					1210:1215	low amount	1206:1215	low amount of GAG	1206:1222	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	9	54	theme	GAG	1220:1222	arg1	GAG					1220:1222	GAG	1220:1222	GAG	1220:1222	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	9	54	theme	GAG	1220:1222	arg1	expression					1169:1178	no expression	1166:1178	no expression of chondrogenic genes	1166:1200	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	11	55	theme	different	1310:1318	arg1	types					1329:1333	The two different collagen types	1302:1333	The two different collagen types in composite scaffolds	1302:1356	The two different collagen types in composite scaffolds yielded similar results.					
31552691	0	56	theme	mesenchymal	58:68	arg1	cells					78:82	human bone marrow-derived mesenchymal stromal cells	32:82	human bone marrow-derived mesenchymal stromal cells	32:82	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	2	57	theme	bone	255:258	arg1	BM-MSCs					299:305	BM-MSCs	299:305	BM-MSCs	299:305	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	2	57	theme	bone	255:258	arg1	cells					292:296	bone marrow-derived mesenchymal stem cells	255:296	chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds	226:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	7	58	theme	sulfated	1000:1007	arg1	glycosaminoglycans					1009:1026	sulfated glycosaminoglycans	1000:1026	sulfated glycosaminoglycans (GAGs)	1000:1033	After 3, 14, and 28 days, the constructs were analyzed with quantitative polymerase chain reaction and confocal microscopy and sulfated glycosaminoglycans (GAGs) were measured.					
31552691	7	58	theme	sulfated	1000:1007	arg1	GAGs					1029:1032	GAGs	1029:1032	GAGs	1029:1032	After 3, 14, and 28 days, the constructs were analyzed with quantitative polymerase chain reaction and confocal microscopy and sulfated glycosaminoglycans (GAGs) were measured.					
31552691	4	59	theme	scaffold	598:605	arg1	rhCo-PLA					607:614	a composite scaffold rhCo-PLA	586:614	a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG)	586:728	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	8	60	theme	culture	1119:1125	arg1	Day					1109:1111	Day 14	1109:1114	Day 14 of culture	1109:1125	The differentiated BM-MSCs entered a hypertrophic state by Day 14 of culture.					
31552691	2	61	theme	chondrogenic	226:237	arg1	differentiation					239:253	chondrogenic differentiation	226:253	chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds	226:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	13	62	theme	cartilage	1604:1612	arg1	regeneration					1614:1625	cartilage regeneration	1604:1625	cartilage regeneration	1604:1625	Thus, caution for cell fate is required when designing cell-biomaterial constructs for cartilage regeneration.					
31552691	9	63	theme	chondrogenic	1183:1194	arg1	genes					1196:1200	chondrogenic genes	1183:1200	chondrogenic genes	1183:1200	The ACs showed dedifferentiation with no expression of chondrogenic genes and low amount of GAG.					
31552691	1	64	theme	biomaterial	147:157	arg1	scaffolds					159:167	biomaterial scaffolds	147:167	biomaterial scaffolds	147:167	Cell therapy combined with biomaterial scaffolds is used to treat cartilage defects.					
31552691	10	65	theme	CG	1229:1230	arg1	membrane					1232:1239	The CG membrane	1225:1239	The CG membrane	1225:1239	The CG membrane induced the highest expression levels of hypertrophic genes.					
31552691	2	66	link	marrow-derived	260:273	arg1	BM-MSCs					299:305	BM-MSCs	299:305	BM-MSCs	299:305	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	2	66	link	marrow-derived	260:273	arg1	cells					292:296	bone marrow-derived mesenchymal stem cells	255:296	chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds	226:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	4	67	theme	I/III	710:714	arg1	CG					726:727	CG	726:727	CG	726:727	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	4	67	theme	I/III	710:714	arg1	membrane					716:723	commercial collagen type I/III membrane	685:723	commercial collagen type I/III membrane (CG)	685:728	The BM-MSCs were then either seeded onto a composite scaffold rhCo-PLA combining polylactide and collagen type II (C2) or type III (C3), or commercial collagen type I/III membrane (CG).					
31552691	11	68	theme	similar	1366:1372	arg1	results					1374:1380	similar results	1366:1380	similar results	1366:1380	The two different collagen types in composite scaffolds yielded similar results.					
31552691	2	69	dep	differentiation	239:253	arg1	BM-MSCs					299:305	BM-MSCs	299:305	BM-MSCs	299:305	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	2	69	dep	differentiation	239:253	arg1	cells					292:296	bone marrow-derived mesenchymal stem cells	255:296	chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds	226:349	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	0	70	theme	cells	78:82	arg1	differentiation					13:27	Chondrogenic differentiation	0:27	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.	0:118	Chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells in a three-dimensional environment.					
31552691	5	71	theme	culture	806:812	arg1	medium					814:819	chondrogenic culture medium	793:819	chondrogenic culture medium	793:819	The BM-MSCs were either cultured in a proliferation medium or chondrogenic culture medium.					
31552691	2	72	theme	cartilage	428:436	arg1	regeneration					438:449	cartilage regeneration	428:449	cartilage regeneration	428:449	We hypothesized that chondrogenic differentiation bone marrow-derived mesenchymal stem cells (BM-MSCs) in three-dimensional biomaterial scaffolds would initiate cartilaginous matrix deposition and prepare the construct for cartilage regeneration in situ.					
31552691	10	73	theme	expression	1261:1270	arg1	levels					1272:1277	the highest expression levels	1249:1277	the highest expression levels of hypertrophic genes	1249:1299	The CG membrane induced the highest expression levels of hypertrophic genes.					
31739485	5	0	contain	possessed	1040:1048	arg2	effect					1076:1081	an enhanced antimicrobial effect	1050:1081	an enhanced antimicrobial effect	1050:1081	The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513).					
31739485	5	0	contain	possessed	1040:1048	arg1	hybrid					1033:1038	the ZnAl/CS hybrid	1021:1038	the ZnAl/CS hybrid	1021:1038	The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513).					
31739485	5	1	dep	coli	1108:1111	arg1	coli					1117:1120	E. coli	1114:1120	E. coli	1114:1120	The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513).					
31739485	5	1	dep	coli	1108:1111	arg1	MTCC					1123:1126	MTCC 739	1123:1130	MTCC 739	1123:1130	The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513).					
31739485	8	2	theme	Al3+	1794:1797	arg1	ions					1805:1808	Zn2+ and Al3+ metal ions	1785:1808	ions	1805:1808	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	2	3	theme	supra-nano	375:384	arg1	material					386:393	an antimicrobial hybrid supra-nano material	351:393	an antimicrobial hybrid supra-nano material	351:393	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	6	4	theme	composite	1203:1211	arg1	CCD					1221:1223	CCD	1221:1223	CCD	1221:1223	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	6	4	theme	composite	1203:1211	arg1	design					1213:1218	the central composite design	1191:1218	the central composite design (CCD) of the response surface methodology (RSM) tool	1191:1271	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	3	5	theme	ZnAl/CS	733:739	arg1	hybrid					741:746	the ZnAl/CS hybrid	729:746	the ZnAl/CS hybrid	729:746	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	6	6	theme	tool	1268:1271	arg1	CCD					1221:1223	CCD	1221:1223	CCD	1221:1223	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	6	6	theme	tool	1268:1271	arg1	design					1213:1218	the central composite design	1191:1218	the central composite design (CCD) of the response surface methodology (RSM) tool	1191:1271	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	8	7	theme	antibacterial	1649:1661	arg1	activity					1663:1670	The antibacterial activity	1645:1670	The antibacterial activity of ZnAl/CS	1645:1681	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	8	7	theme	antibacterial	1649:1661	arg1	due					1694:1696	due	1694:1696	due	1694:1696	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	1	8	theme	new	189:191	arg1	antibiotics					193:203	new antibiotics	189:203	new antibiotics	189:203	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	1	8	theme	new	189:191	arg1	organic-inorganic					266:282	hybrid organic-inorganic	259:282	hybrid organic-inorganic	259:282	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	4	9	theme	individual	889:898	arg1	ZnAl					911:914	ZnAl	911:914	ZnAl	911:914	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	4	9	theme	individual	889:898	arg1	components					900:909	the individual components ZnAl and CS	885:921	the individual components ZnAl and CS	885:921	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	4	9	theme	individual	889:898	arg1	CS					920:921	CS	920:921	CS	920:921	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	8	10	theme	ZnO	1825:1827	arg1	compounds					1849:1857	ZnO, Al2O3, and ZnAl2O4 compounds	1825:1857	ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation	1825:1898	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	8	11	theme	Al2O3	1830:1834	arg1	compounds					1849:1857	ZnO, Al2O3, and ZnAl2O4 compounds	1825:1857	ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation	1825:1898	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	1	12	theme	alternative	217:227	arg1	sources					243:249	alternative and effective sources	217:249	alternative and effective sources such as hybrid organic-inorganic	217:282	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	1	12	theme	alternative	217:227	arg1	organic-inorganic					266:282	hybrid organic-inorganic	259:282	hybrid organic-inorganic	259:282	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	4	13	theme	ZnAl/CS	967:973	arg1	hybrid					975:980	the ZnAl/CS hybrid	963:980	the ZnAl/CS hybrid	963:980	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	6	14	theme	surface	1242:1248	arg1	RSM					1263:1265	RSM	1263:1265	RSM	1263:1265	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	6	14	theme	surface	1242:1248	arg1	methodology					1250:1260	the response surface methodology	1229:1260	the response surface methodology (RSM) tool	1229:1271	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	6	15	theme	reaction	1313:1320	arg1	parameters					1278:1287	the parameters	1274:1287	the parameters of the hybrid synthesis reaction	1274:1320	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	6	16	theme	Zn/Al	1407:1411	arg1	ratio					1413:1417	reagent Zn/Al ratio	1399:1417	reagent Zn/Al ratio	1399:1417	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	1	17	theme	effective	233:241	arg1	sources					243:249	alternative and effective sources	217:249	alternative and effective sources such as hybrid organic-inorganic	217:282	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	1	17	theme	effective	233:241	arg1	organic-inorganic					266:282	hybrid organic-inorganic	259:282	hybrid organic-inorganic	259:282	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	3	18	theme	high	781:784	arg1	stability					794:802	high thermal stability	781:802	high thermal stability	781:802	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	6	19	theme	hybrid	1296:1301	arg1	reaction					1313:1320	the hybrid synthesis reaction	1292:1320	the hybrid synthesis reaction	1292:1320	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	4	20	theme	successful	937:946	arg1	preparation					948:958	a successful preparation	935:958	a successful preparation of the ZnAl/CS hybrid	935:980	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	8	21	theme	antibacterial	1725:1737	arg1	activity					1739:1746	the antibacterial activity	1721:1746	the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation	1721:1898	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	0	22	theme	Antibacterial	73:85	arg1	Material					87:94	Efficient Antibacterial Material	63:94	Efficient Antibacterial Material	63:94	Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material.					
31739485	0	23	theme	ZnAl/Chitosan	16:28	arg1	Preparation					48:58	ZnAl/Chitosan Supra-Nano Hybrid Preparation	16:58	ZnAl/Chitosan Supra-Nano Hybrid Preparation	16:58	Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material.					
31739485	8	24	theme	preparation	1888:1898	arg1	method					1878:1883	the method	1874:1883	the method of preparation	1874:1898	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	8	25	theme	ions	1805:1808	arg1	release					1774:1780	the release	1770:1780	the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation	1770:1898	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	1	26	theme	resistance	125:134	arg1	menace					101:106	The menace	97:106	The menace of antimicrobial resistance	97:134	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	7	27	theme	%	1642:1642	arg1	zone					1624:1627	the inhibition zone	1609:1627	the inhibition zone of about 41.6%	1609:1642	After optimization, it was found that the antibacterial activity of ZnAl/CS was strengthened against E. coli as evidenced by a widening of the inhibition zone of about 41.6%.					
31739485	7	28	theme	antibacterial	1512:1524	arg1	activity					1526:1533	the antibacterial activity	1508:1533	the antibacterial activity of ZnAl/CS	1508:1544	After optimization, it was found that the antibacterial activity of ZnAl/CS was strengthened against E. coli as evidenced by a widening of the inhibition zone of about 41.6%.					
31739485	2	29	theme	synthesis	428:436	arg1	method					438:443	the bi-titration synthesis method	411:443	the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide	411:494	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	2	30	theme	ZnAl	466:469	arg1	hydroxide					486:494	ZnAl layered double hydroxide	466:494	ZnAl layered double hydroxide	466:494	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	7	31	theme	zone	1624:1627	arg1	widening					1597:1604	a widening	1595:1604	a widening of the inhibition zone of about 41.6%	1595:1642	After optimization, it was found that the antibacterial activity of ZnAl/CS was strengthened against E. coli as evidenced by a widening of the inhibition zone of about 41.6%.					
31739485	6	32	theme	reagent	1399:1405	arg1	ratio					1413:1417	reagent Zn/Al ratio	1399:1417	reagent Zn/Al ratio	1399:1417	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	1	33	theme	other	316:320	arg1	combinations					322:333	other combinations	316:333	other combinations	316:333	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	1	33	theme	other	316:320	arg1	organic-inorganic					266:282	hybrid organic-inorganic	259:282	hybrid organic-inorganic	259:282	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	5	34	theme	antibacterial	987:999	arg1	tests					1001:1005	The antibacterial tests	983:1005	The antibacterial tests	983:1005	The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513).					
31739485	0	35	theme	Hybrid	41:46	arg1	Preparation					48:58	ZnAl/Chitosan Supra-Nano Hybrid Preparation	16:58	ZnAl/Chitosan Supra-Nano Hybrid Preparation	16:58	Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material.					
31739485	5	36	theme	antimicrobial	1062:1074	arg1	effect					1076:1081	an enhanced antimicrobial effect	1050:1081	an enhanced antimicrobial effect	1050:1081	The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513).					
31739485	2	37	theme	double	479:484	arg1	hydroxide					486:494	ZnAl layered double hydroxide	466:494	ZnAl layered double hydroxide	466:494	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	5	38	dep	Penicilliumcyclopium	1137:1156	arg1	cyclopium					1162:1170	P. cyclopium	1159:1170	P. cyclopium	1159:1170	The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513).					
31739485	4	39	theme	characteristic	856:869	arg1	properties					871:880	the characteristic properties	852:880	the characteristic properties of the individual components ZnAl and CS	852:921	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	3	40	theme	spectrometer	524:535	arg1	analyses					705:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	4	41	dep	components	900:909	arg1	ZnAl					911:914	ZnAl	911:914	ZnAl	911:914	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	4	41	dep	components	900:909	arg1	components					900:909	the individual components ZnAl and CS	885:921	the individual components ZnAl and CS	885:921	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	4	41	dep	components	900:909	arg1	CS					920:921	CS	920:921	CS	920:921	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	3	42	theme	X-ray	641:645	arg1	XRD					660:662	XRD	660:662	XRD	660:662	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	42	theme	X-ray	641:645	arg1	diffraction					647:657	X-ray diffraction	641:657	X-ray diffraction (XRD)	641:663	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	43	theme	Fourier-transform	497:513	arg1	spectrometer					524:535	Fourier-transform infrared spectrometer	497:535	Fourier-transform infrared spectrometer (FTIR)	497:542	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	43	theme	Fourier-transform	497:513	arg1	FTIR					538:541	FTIR	538:541	FTIR	538:541	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	44	theme	electron	679:686	arg1	SEM					700:702	SEM	700:702	SEM	700:702	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	44	theme	electron	679:686	arg1	microscopy					688:697	scanning electron microscopy	670:697	scanning electron microscopy (SEM)	670:703	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	4	45	theme	ZnAl/CS	820:826	arg1	characterization					828:843	ZnAl/CS characterization	820:843	ZnAl/CS characterization	820:843	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	8	46	theme	metal	1799:1803	arg1	ions					1805:1808	Zn2+ and Al3+ metal ions	1785:1808	ions	1805:1808	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	4	47	theme	components	900:909	arg1	properties					871:880	the characteristic properties	852:880	the characteristic properties of the individual components ZnAl and CS	852:921	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	1	48	theme	organic-organic	285:299	arg1	organic-inorganic					266:282	hybrid organic-inorganic	259:282	hybrid organic-inorganic	259:282	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	1	48	theme	organic-organic	285:299	arg1	materials					301:309	organic-organic materials	285:309	organic-organic materials	285:309	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	6	49	theme	central	1195:1201	arg1	CCD					1221:1223	CCD	1221:1223	CCD	1221:1223	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	6	49	theme	central	1195:1201	arg1	design					1213:1218	the central composite design	1191:1218	the central composite design (CCD) of the response surface methodology (RSM) tool	1191:1271	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	2	50	theme	hybrid	368:373	arg1	material					386:393	an antimicrobial hybrid supra-nano material	351:393	an antimicrobial hybrid supra-nano material	351:393	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	3	51	dep	ultraviolet-visible	611:629	arg1	UV-Vis					632:637	UV-Vis	632:637	UV-Vis	632:637	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	6	52	theme	methodology	1250:1260	arg1	tool					1268:1271	the response surface methodology (RSM) tool	1229:1271	the response surface methodology (RSM) tool	1229:1271	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	8	53	theme	ZnAl/CS	1675:1681	arg1	activity					1663:1670	The antibacterial activity	1645:1670	The antibacterial activity of ZnAl/CS	1645:1681	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	8	53	theme	ZnAl/CS	1675:1681	arg1	due					1694:1696	due	1694:1696	due	1694:1696	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	3	54	theme	low	758:760	arg1	crystallinity					762:774	low crystallinity	758:774	low crystallinity	758:774	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	8	55	theme	ZnAl2O4	1841:1847	arg1	compounds					1849:1857	ZnO, Al2O3, and ZnAl2O4 compounds	1825:1857	ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation	1825:1898	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	6	56	theme	response	1233:1240	arg1	RSM					1263:1265	RSM	1263:1265	RSM	1263:1265	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	6	56	theme	response	1233:1240	arg1	methodology					1250:1260	the response surface methodology	1229:1260	the response surface methodology (RSM) tool	1229:1271	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	6	57	theme	synthesis	1303:1311	arg1	reaction					1313:1320	the hybrid synthesis reaction	1292:1320	the hybrid synthesis reaction	1292:1320	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	3	58	theme	ultraviolet-visible	611:629	arg1	analyses					705:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	59	theme	thermal	786:792	arg1	stability					794:802	high thermal stability	781:802	high thermal stability	781:802	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	60	theme	diffraction	647:657	arg1	analyses					705:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	6	61	theme	reaction	1377:1384	arg1	pH					1386:1387	reaction pH	1377:1387	reaction pH	1377:1387	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	7	62	theme	ZnAl/CS	1538:1544	arg1	activity					1526:1533	the antibacterial activity	1508:1533	the antibacterial activity of ZnAl/CS	1508:1544	After optimization, it was found that the antibacterial activity of ZnAl/CS was strengthened against E. coli as evidenced by a widening of the inhibition zone of about 41.6%.					
31739485	8	63	theme	activity	1739:1746	arg1	reactivation					1705:1716	the reactivation	1701:1716	the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation	1701:1898	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	8	64	theme	CS	1751:1752	arg1	activity					1739:1746	the antibacterial activity	1721:1746	the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation	1721:1898	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	4	65	theme	hybrid	975:980	arg1	preparation					948:958	a successful preparation	935:958	a successful preparation of the ZnAl/CS hybrid	935:980	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	2	66	theme	bi-titration	415:426	arg1	method					438:443	the bi-titration synthesis method	411:443	the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide	411:494	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	0	67	theme	Supra-Nano	30:39	arg1	Preparation					48:58	ZnAl/Chitosan Supra-Nano Hybrid Preparation	16:58	ZnAl/Chitosan Supra-Nano Hybrid Preparation	16:58	Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material.					
31739485	8	68	theme	Zn2+	1785:1788	arg1	ions					1805:1808	Zn2+ and Al3+ metal ions	1785:1808	ions	1805:1808	The antibacterial activity of ZnAl/CS was mainly due to the reactivation of the antibacterial activity of CS associated with the release of Zn2+ and Al3+ metal ions in addition to ZnO, Al2O3, and ZnAl2O4 compounds resulting from the method of preparation.					
31739485	1	69	theme	antimicrobial	111:123	arg1	resistance					125:134	antimicrobial resistance	111:134	antimicrobial resistance	111:134	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
31739485	2	70	theme	chitosan	448:455	arg1	method					438:443	the bi-titration synthesis method	411:443	the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide	411:494	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	0	71	theme	Preparation	48:58	arg1	Optimization					0:11	Optimization	0:11	Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material.	0:95	Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material.					
31739485	5	72	theme	enhanced	1053:1060	arg1	effect					1076:1081	an enhanced antimicrobial effect	1050:1081	an enhanced antimicrobial effect	1050:1081	The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513).					
31739485	7	73	theme	inhibition	1613:1622	arg1	zone					1624:1627	the inhibition zone	1609:1627	the inhibition zone of about 41.6%	1609:1642	After optimization, it was found that the antibacterial activity of ZnAl/CS was strengthened against E. coli as evidenced by a widening of the inhibition zone of about 41.6%.					
31739485	2	74	theme	hydroxide	486:494	arg1	method					438:443	the bi-titration synthesis method	411:443	the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide	411:494	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	4	75	theme	characterization	828:843	arg1	results					809:815	The results	805:815	The results of ZnAl/CS characterization	805:843	The results of ZnAl/CS characterization showed the characteristic properties of the individual components ZnAl and CS, indicating a successful preparation of the ZnAl/CS hybrid.					
31739485	0	76	theme	Efficient	63:71	arg1	Material					87:94	Efficient Antibacterial Material	63:94	Efficient Antibacterial Material	63:94	Optimization of ZnAl/Chitosan Supra-Nano Hybrid Preparation as Efficient Antibacterial Material.					
31739485	2	77	theme	layered	471:477	arg1	hydroxide					486:494	ZnAl layered double hydroxide	466:494	ZnAl layered double hydroxide	466:494	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	3	78	theme	microscopy	688:697	arg1	analyses					705:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	2	79	theme	antimicrobial	354:366	arg1	material					386:393	an antimicrobial hybrid supra-nano material	351:393	an antimicrobial hybrid supra-nano material	351:393	In this study, an antimicrobial hybrid supra-nano material was prepared by the bi-titration synthesis method of chitosan (CS) and ZnAl layered double hydroxide.					
31739485	5	80	theme	ZnAl/CS	1025:1031	arg1	hybrid					1033:1038	the ZnAl/CS hybrid	1021:1038	the ZnAl/CS hybrid	1021:1038	The antibacterial tests revealed that the ZnAl/CS hybrid possessed an enhanced antimicrobial effect against both Escherichia coli (E. coli, MTCC 739) and Penicilliumcyclopium (P. cyclopium, AS 3.4513).					
31739485	3	81	theme	scanning	670:677	arg1	SEM					700:702	SEM	700:702	SEM	700:702	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	81	theme	scanning	670:677	arg1	microscopy					688:697	scanning electron microscopy	670:697	scanning electron microscopy (SEM)	670:703	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	82	theme	infrared	515:522	arg1	spectrometer					524:535	Fourier-transform infrared spectrometer	497:535	Fourier-transform infrared spectrometer (FTIR)	497:542	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	82	theme	infrared	515:522	arg1	FTIR					538:541	FTIR	538:541	FTIR	538:541	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	3	83	theme	thermogravimetric	545:561	arg1	analyses					705:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses	497:712	Fourier-transform infrared spectrometer (FTIR), thermogravimetric and differential thermal gravimetric (TGA/DTG), ultraviolet-visible (UV-Vis), X-ray diffraction (XRD), and scanning electron microscopy (SEM) analyses indicated that the ZnAl/CS hybrid exhibited low crystallinity with high thermal stability.					
31739485	6	84	theme	chitosan	1433:1440	arg1	concentration					1442:1454	chitosan concentration	1433:1454	chitosan concentration	1433:1454	Under the central composite design (CCD) of the response surface methodology (RSM) tool, the parameters of the hybrid synthesis reaction were optimized and the result obtained was as follows: reaction pH was 11.3, reagent Zn/Al ratio was 3.27, and chitosan concentration was 1.07 g/L.					
31739485	1	85	theme	hybrid	259:264	arg1	organic-inorganic					266:282	hybrid organic-inorganic	259:282	hybrid organic-inorganic	259:282	The menace of antimicrobial resistance continues to increase and hence the need to discover new antibiotics, especially alternative and effective sources such as hybrid organic-inorganic, organic-organic materials, and other combinations.					
30951951	12	0	theme	colonic	1539:1545	arg1	contractions					1550:1561	colonic SM contractions	1539:1561	colonic SM contractions	1539:1561	In conclusion, DFO increased colonic SM contractions without morphological change and acted as a bulk-forming and stimulant laxative to increase fecal output and intestinal motility.					
30951951	10	1	theme	SM	1394:1395	arg1	contractions					1397:1408	colonic SM contractions	1386:1408	colonic SM contractions	1386:1408	DFO also increased the amplitude and duration of colonic SM contractions.					
30951951	6	2	theme	colons	829:834	arg1	contractions					780:791	smooth muscle (SM) contractions	761:791	smooth muscle (SM) contractions	761:791	Gastrointestinal transits were analysed and motility patterns, smooth muscle (SM) contractions and morphological structures of the colons were assessed.					
30951951	6	2	theme	colons	829:834	arg1	structures					811:820	morphological structures	797:820	morphological structures of the colons	797:834	Gastrointestinal transits were analysed and motility patterns, smooth muscle (SM) contractions and morphological structures of the colons were assessed.					
30951951	6	2	theme	colons	829:834	arg1	patterns					751:758	motility patterns	742:758	motility patterns	742:758	Gastrointestinal transits were analysed and motility patterns, smooth muscle (SM) contractions and morphological structures of the colons were assessed.					
30951951	8	3	theme	transit	1019:1025	arg1	time					1027:1030	gut transit time	1015:1030	gut transit time	1015:1030	In mice treated with FOS and bifidobacteria, gut transit time was reduced, while upper gut transit was increased in comparison to DFO groups.					
30951951	1	4	contain	has	109:111	arg1	oligosaccharide					87:101	Dragon fruit oligosaccharide	74:101	Dragon fruit oligosaccharide (DFO)	74:107	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	1	4	contain	has	109:111	arg2	property					125:132	a prebiotic property	113:132	a prebiotic property which improves gut health by selectively stimulating the colonic microbiota	113:208	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	1	4	contain	has	109:111	arg1	DFO					104:106	DFO	104:106	DFO	104:106	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	8	5	theme	gut	1015:1017	arg1	time					1027:1030	gut transit time	1015:1030	gut transit time	1015:1030	In mice treated with FOS and bifidobacteria, gut transit time was reduced, while upper gut transit was increased in comparison to DFO groups.					
30951951	7	6	theme	fecal	921:925	arg1	output					927:932	fecal output	921:932	fecal output	921:932	Administration of FOS, 500 and 1000 mg/kg DFO significantly increased fecal output when compared to the control group.					
30951951	1	7	theme	gut	149:151	arg1	health					153:158	gut health	149:158	gut health	149:158	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	3	8	theme	motor	344:348	arg1	functions					350:358	gut motor functions	340:358	gut motor functions	340:358	However, no study has been done to understand the DFO effects on gut motor functions.					
30951951	4	9	theme	probiotic	491:499	arg1	bifidobacteria					501:514	probiotic bifidobacteria	491:514	probiotic bifidobacteria	491:514	This research thus aimed to investigate the DFO effects on mice colons compared to the prebiotic fructo-oligosaccharide (FOS) and probiotic bifidobacteria.					
30951951	6	10	theme	muscle	768:773	arg1	contractions					780:791	smooth muscle (SM) contractions	761:791	smooth muscle (SM) contractions	761:791	Gastrointestinal transits were analysed and motility patterns, smooth muscle (SM) contractions and morphological structures of the colons were assessed.					
30951951	9	11	theme	pellet	1243:1248	arg1	velocity					1250:1257	fecal pellet velocity	1237:1257	fecal pellet velocity	1237:1257	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	9	12	theme	colonic	1196:1202	arg1	contractions					1220:1231	colonic non-propagation contractions	1196:1231	colonic non-propagation contractions	1196:1231	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	0	13	from	motility	56:63	arg1	mice					68:71	mice	68:71	mice	68:71	Prebiotic oligosaccharides from dragon fruits alter gut motility in mice.					
30951951	5	14	theme	CFU	619:621	arg1	animalis					639:646	109 CFU Bifidobacterium animalis	615:646	109 CFU Bifidobacterium animalis	615:646	The mice in this study received distilled water; 100, 500, and 1000 mg/kg DFO; 1000 mg/kg FOS; or 109 CFU Bifidobacterium animalis daily for 1 week and some treatments for 2 weeks.					
30951951	6	15	theme	smooth	761:766	arg1	SM					776:777	SM	776:777	SM	776:777	Gastrointestinal transits were analysed and motility patterns, smooth muscle (SM) contractions and morphological structures of the colons were assessed.					
30951951	6	15	theme	smooth	761:766	arg1	muscle					768:773	smooth muscle	761:773	smooth muscle (SM) contractions	761:791	Gastrointestinal transits were analysed and motility patterns, smooth muscle (SM) contractions and morphological structures of the colons were assessed.					
30951951	4	16	theme	prebiotic	448:456	arg1	FOS					482:484	FOS	482:484	FOS	482:484	This research thus aimed to investigate the DFO effects on mice colons compared to the prebiotic fructo-oligosaccharide (FOS) and probiotic bifidobacteria.					
30951951	4	16	theme	prebiotic	448:456	arg1	fructo-oligosaccharide					458:479	the prebiotic fructo-oligosaccharide	444:479	the prebiotic fructo-oligosaccharide (FOS)	444:485	This research thus aimed to investigate the DFO effects on mice colons compared to the prebiotic fructo-oligosaccharide (FOS) and probiotic bifidobacteria.					
30951951	12	17	theme	morphological	1571:1583	arg1	change					1585:1590	morphological change	1571:1590	morphological change	1571:1590	In conclusion, DFO increased colonic SM contractions without morphological change and acted as a bulk-forming and stimulant laxative to increase fecal output and intestinal motility.					
30951951	5	18	from	mice	521:524	arg1	study					534:538	this study	529:538	this study	529:538	The mice in this study received distilled water; 100, 500, and 1000 mg/kg DFO; 1000 mg/kg FOS; or 109 CFU Bifidobacterium animalis daily for 1 week and some treatments for 2 weeks.					
30951951	3	19	theme	gut	340:342	arg1	functions					350:358	gut motor functions	340:358	gut motor functions	340:358	However, no study has been done to understand the DFO effects on gut motor functions.					
30951951	9	20	theme	wall	1143:1146	arg1	motions					1148:1154	colonic wall motions	1135:1154	colonic wall motions	1135:1154	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	7	21	theme	1000 mg/kg	882:891	arg1	DFO					893:895	1000 mg/kg DFO	882:895	1000 mg/kg DFO	882:895	Administration of FOS, 500 and 1000 mg/kg DFO significantly increased fecal output when compared to the control group.					
30951951	1	22	theme	Dragon	74:79	arg1	oligosaccharide					87:101	Dragon fruit oligosaccharide	74:101	Dragon fruit oligosaccharide (DFO)	74:107	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	1	22	theme	Dragon	74:79	arg1	DFO					104:106	DFO	104:106	DFO	104:106	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	12	23	theme	bulk-forming	1607:1618	arg1	laxative					1634:1641	a bulk-forming and stimulant laxative	1605:1641	a bulk-forming and stimulant laxative	1605:1641	In conclusion, DFO increased colonic SM contractions without morphological change and acted as a bulk-forming and stimulant laxative to increase fecal output and intestinal motility.					
30951951	12	23	theme	bulk-forming	1607:1618	arg1	DFO					1525:1527	DFO	1525:1527	DFO	1525:1527	In conclusion, DFO increased colonic SM contractions without morphological change and acted as a bulk-forming and stimulant laxative to increase fecal output and intestinal motility.					
30951951	0	24	from	fruits	39:44	arg1	oligosaccharides					10:25	Prebiotic oligosaccharides	0:25	Prebiotic oligosaccharides from dragon fruits	0:44	Prebiotic oligosaccharides from dragon fruits alter gut motility in mice.					
30951951	5	25	theme	1000 mg/kg	596:605	arg1	FOS					607:609	1000 mg/kg FOS	596:609	1000 mg/kg FOS	596:609	The mice in this study received distilled water; 100, 500, and 1000 mg/kg DFO; 1000 mg/kg FOS; or 109 CFU Bifidobacterium animalis daily for 1 week and some treatments for 2 weeks.					
30951951	6	26	theme	motility	742:749	arg1	patterns					751:758	motility patterns	742:758	motility patterns	742:758	Gastrointestinal transits were analysed and motility patterns, smooth muscle (SM) contractions and morphological structures of the colons were assessed.					
30951951	1	27	theme	fruit	81:85	arg1	oligosaccharide					87:101	Dragon fruit oligosaccharide	74:101	Dragon fruit oligosaccharide (DFO)	74:107	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	1	27	theme	fruit	81:85	arg1	DFO					104:106	DFO	104:106	DFO	104:106	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	11	28	theme	normal	1438:1443	arg1	epithelia					1445:1453	normal epithelia	1438:1453	normal epithelia	1438:1453	Histological stains showed normal epithelia, crypts, goblet cells, and SM thickness in all groups.					
30951951	0	29	theme	Prebiotic	0:8	arg1	oligosaccharides					10:25	Prebiotic oligosaccharides	0:25	Prebiotic oligosaccharides from dragon fruits	0:44	Prebiotic oligosaccharides from dragon fruits alter gut motility in mice.					
30951951	11	30	theme	SM	1482:1483	arg1	thickness					1485:1493	SM thickness	1482:1493	SM thickness	1482:1493	Histological stains showed normal epithelia, crypts, goblet cells, and SM thickness in all groups.					
30951951	9	31	theme	non-propagation	1204:1218	arg1	contractions					1220:1231	colonic non-propagation contractions	1196:1231	colonic non-propagation contractions	1196:1231	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	9	32	theme	motions	1148:1154	arg1	maps					1127:1130	Spatiotemporal maps	1112:1130	Spatiotemporal maps of colonic wall motions	1112:1154	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	11	33	theme	Histological	1411:1422	arg1	stains					1424:1429	Histological stains	1411:1429	Histological stains	1411:1429	Histological stains showed normal epithelia, crypts, goblet cells, and SM thickness in all groups.					
30951951	10	34	theme	colonic	1386:1392	arg1	contractions					1397:1408	colonic SM contractions	1386:1408	colonic SM contractions	1386:1408	DFO also increased the amplitude and duration of colonic SM contractions.					
30951951	0	35	theme	dragon	32:37	arg1	fruits					39:44	dragon fruits	32:44	dragon fruits	32:44	Prebiotic oligosaccharides from dragon fruits alter gut motility in mice.					
30951951	9	36	theme	Spatiotemporal	1112:1125	arg1	maps					1127:1130	Spatiotemporal maps	1112:1130	Spatiotemporal maps of colonic wall motions	1112:1154	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	10	37	theme	contractions	1397:1408	arg1	duration					1374:1381	duration	1374:1381	duration	1374:1381	DFO also increased the amplitude and duration of colonic SM contractions.					
30951951	10	37	theme	contractions	1397:1408	arg1	amplitude					1360:1368	amplitude	1360:1368	amplitude	1360:1368	DFO also increased the amplitude and duration of colonic SM contractions.					
30951951	7	38	theme	control	955:961	arg1	group					963:967	the control group	951:967	the control group	951:967	Administration of FOS, 500 and 1000 mg/kg DFO significantly increased fecal output when compared to the control group.					
30951951	12	39	theme	intestinal	1672:1681	arg1	motility					1683:1690	intestinal motility	1672:1690	intestinal motility	1672:1690	In conclusion, DFO increased colonic SM contractions without morphological change and acted as a bulk-forming and stimulant laxative to increase fecal output and intestinal motility.					
30951951	1	40	theme	colonic	191:197	arg1	microbiota					199:208	the colonic microbiota	187:208	the colonic microbiota	187:208	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	12	41	theme	SM	1547:1548	arg1	contractions					1550:1561	colonic SM contractions	1539:1561	colonic SM contractions	1539:1561	In conclusion, DFO increased colonic SM contractions without morphological change and acted as a bulk-forming and stimulant laxative to increase fecal output and intestinal motility.					
30951951	10	42	dep	amplitude	1360:1368	arg1	the					1356:1358	the	1356:1358	the	1356:1358	DFO also increased the amplitude and duration of colonic SM contractions.					
30951951	9	43	with	consistent	1260:1269	arg1	results					1280:1286	the results	1276:1286	the results from groups treated with FOS and bifidobacteria	1276:1334	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	8	44	theme	DFO	1100:1102	arg1	groups					1104:1109	DFO groups	1100:1109	DFO groups	1100:1109	In mice treated with FOS and bifidobacteria, gut transit time was reduced, while upper gut transit was increased in comparison to DFO groups.					
30951951	13	45	theme	dietary	1708:1714	arg1	supplement					1716:1725	a dietary supplement	1706:1725	a dietary supplement	1706:1725	Thus, DFO as a dietary supplement may promote gut health and correct gastrointestinal motility disorders.					
30951951	5	46	theme	Bifidobacterium	623:637	arg1	animalis					639:646	109 CFU Bifidobacterium animalis	615:646	109 CFU Bifidobacterium animalis	615:646	The mice in this study received distilled water; 100, 500, and 1000 mg/kg DFO; 1000 mg/kg FOS; or 109 CFU Bifidobacterium animalis daily for 1 week and some treatments for 2 weeks.					
30951951	6	47	theme	morphological	797:809	arg1	structures					811:820	morphological structures	797:820	morphological structures of the colons	797:834	Gastrointestinal transits were analysed and motility patterns, smooth muscle (SM) contractions and morphological structures of the colons were assessed.					
30951951	2	48	theme	microbiota	220:229	arg1	composition					231:241	microbiota composition	220:241	microbiota composition	220:241	Altering microbiota composition may affect intestinal motility.					
30951951	8	49	theme	gut	1057:1059	arg1	transit					1061:1067	upper gut transit	1051:1067	upper gut transit	1051:1067	In mice treated with FOS and bifidobacteria, gut transit time was reduced, while upper gut transit was increased in comparison to DFO groups.					
30951951	0	50	theme	gut	52:54	arg1	motility					56:63	gut motility	52:63	gut motility in mice	52:71	Prebiotic oligosaccharides from dragon fruits alter gut motility in mice.					
30951951	12	51	theme	fecal	1655:1659	arg1	output					1661:1666	fecal output	1655:1666	fecal output	1655:1666	In conclusion, DFO increased colonic SM contractions without morphological change and acted as a bulk-forming and stimulant laxative to increase fecal output and intestinal motility.					
30951951	9	52	from	groups	1293:1298	arg1	results					1280:1286	the results	1276:1286	the results from groups treated with FOS and bifidobacteria	1276:1334	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	6	53	theme	Gastrointestinal	698:713	arg1	transits					715:722	Gastrointestinal transits	698:722	Gastrointestinal transits	698:722	Gastrointestinal transits were analysed and motility patterns, smooth muscle (SM) contractions and morphological structures of the colons were assessed.					
30951951	7	54	theme	FOS	869:871	arg1	DFO					893:895	1000 mg/kg DFO	882:895	1000 mg/kg DFO	882:895	Administration of FOS, 500 and 1000 mg/kg DFO significantly increased fecal output when compared to the control group.					
30951951	7	54	theme	FOS	869:871	arg1	Administration					851:864	Administration	851:864	Administration of FOS	851:871	Administration of FOS, 500 and 1000 mg/kg DFO significantly increased fecal output when compared to the control group.					
30951951	4	55	from	effects	409:415	arg1	colons					425:430	mice colons	420:430	mice colons	420:430	This research thus aimed to investigate the DFO effects on mice colons compared to the prebiotic fructo-oligosaccharide (FOS) and probiotic bifidobacteria.					
30951951	4	56	theme	DFO	405:407	arg1	effects					409:415	the DFO effects	401:415	the DFO effects on mice colons compared to the prebiotic fructo-oligosaccharide (FOS) and probiotic bifidobacteria	401:514	This research thus aimed to investigate the DFO effects on mice colons compared to the prebiotic fructo-oligosaccharide (FOS) and probiotic bifidobacteria.					
30951951	5	57	theme	distilled	549:557	arg1	water					559:563	distilled water	549:563	distilled water	549:563	The mice in this study received distilled water; 100, 500, and 1000 mg/kg DFO; 1000 mg/kg FOS; or 109 CFU Bifidobacterium animalis daily for 1 week and some treatments for 2 weeks.					
30951951	9	58	theme	colonic	1135:1141	arg1	motions					1148:1154	colonic wall motions	1135:1154	colonic wall motions	1135:1154	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	8	59	theme	upper	1051:1055	arg1	transit					1061:1067	upper gut transit	1051:1067	upper gut transit	1051:1067	In mice treated with FOS and bifidobacteria, gut transit time was reduced, while upper gut transit was increased in comparison to DFO groups.					
30951951	4	60	theme	mice	420:423	arg1	colons					425:430	mice colons	420:430	mice colons	420:430	This research thus aimed to investigate the DFO effects on mice colons compared to the prebiotic fructo-oligosaccharide (FOS) and probiotic bifidobacteria.					
30951951	1	61	theme	prebiotic	115:123	arg1	property					125:132	a prebiotic property	113:132	a prebiotic property which improves gut health by selectively stimulating the colonic microbiota	113:208	Dragon fruit oligosaccharide (DFO) has a prebiotic property which improves gut health by selectively stimulating the colonic microbiota.					
30951951	13	62	theme	motility	1779:1786	arg1	disorders					1788:1796	gastrointestinal motility disorders	1762:1796	gastrointestinal motility disorders	1762:1796	Thus, DFO as a dietary supplement may promote gut health and correct gastrointestinal motility disorders.					
30951951	3	63	theme	DFO	325:327	arg1	effects					329:335	the DFO effects	321:335	the DFO effects on gut motor functions	321:358	However, no study has been done to understand the DFO effects on gut motor functions.					
30951951	3	64	from	effects	329:335	arg1	functions					350:358	gut motor functions	340:358	gut motor functions	340:358	However, no study has been done to understand the DFO effects on gut motor functions.					
30951951	2	65	theme	intestinal	254:263	arg1	motility					265:272	intestinal motility	254:272	intestinal motility	254:272	Altering microbiota composition may affect intestinal motility.					
30951951	12	66	theme	stimulant	1624:1632	arg1	laxative					1634:1641	a bulk-forming and stimulant laxative	1605:1641	a bulk-forming and stimulant laxative	1605:1641	In conclusion, DFO increased colonic SM contractions without morphological change and acted as a bulk-forming and stimulant laxative to increase fecal output and intestinal motility.					
30951951	12	66	theme	stimulant	1624:1632	arg1	DFO					1525:1527	DFO	1525:1527	DFO	1525:1527	In conclusion, DFO increased colonic SM contractions without morphological change and acted as a bulk-forming and stimulant laxative to increase fecal output and intestinal motility.					
30951951	9	67	theme	fecal	1237:1241	arg1	velocity					1250:1257	fecal pellet velocity	1237:1257	fecal pellet velocity	1237:1257	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	13	68	theme	gut	1739:1741	arg1	health					1743:1748	gut health	1739:1748	gut health	1739:1748	Thus, DFO as a dietary supplement may promote gut health and correct gastrointestinal motility disorders.					
30951951	9	69	theme	contractions	1220:1231	arg1	velocity					1250:1257	fecal pellet velocity	1237:1257	fecal pellet velocity	1237:1257	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	9	69	theme	contractions	1220:1231	arg1	number					1186:1191	the number	1182:1191	the number of colonic non-propagation contractions	1182:1231	Spatiotemporal maps of colonic wall motions showed that DFO increased the number of colonic non-propagation contractions and fecal pellet velocity, consistent with the results from groups treated with FOS and bifidobacteria.					
30951951	13	70	theme	gastrointestinal	1762:1777	arg1	disorders					1788:1796	gastrointestinal motility disorders	1762:1796	gastrointestinal motility disorders	1762:1796	Thus, DFO as a dietary supplement may promote gut health and correct gastrointestinal motility disorders.					
30951951	11	71	theme	goblet	1464:1469	arg1	cells					1471:1475	goblet cells	1464:1475	goblet cells	1464:1475	Histological stains showed normal epithelia, crypts, goblet cells, and SM thickness in all groups.					
29969488	6	0	theme	Bacteroidetes	1198:1210	arg1	ratio					1212:1216	Bacteroidetes ratio	1198:1216	Bacteroidetes ratio	1198:1216	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	7	1	theme	protein	1418:1424	arg1	concentration					1426:1438	Milk protein concentration	1413:1438	Milk protein concentration	1413:1438	Milk protein concentration decreased with RS diet and lactose concentration increased within the first weeks of lactation while decreased the week before weaning with the RS diet.					
29969488	4	2	theme	milk	809:812	arg1	composition					814:824	milk composition	809:824	milk composition	809:824	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	11	3	from	Changes	2071:2077	arg1	microbiota					2086:2095	the microbiota	2082:2095	the microbiota transferred from mother to piglets due to the inclusion of RS in the maternal diet	2082:2178	CONCLUSION Changes in the microbiota transferred from mother to piglets due to the inclusion of RS in the maternal diet are rather limited even though milk composition was affected.					
29969488	8	4	theme	dietary	1610:1616	arg1	treatment					1618:1626	the dietary treatment	1606:1626	the dietary treatment	1606:1626	No effect of the dietary treatment, on piglets' bodyweight or diarrhoea frequency post-weaning was observed.					
29969488	6	5	theme	colonic	1376:1382	arg1	microbiota					1384:1393	the colonic microbiota	1372:1393	the colonic microbiota of their progeny	1372:1410	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	9	6	from	cytokines	1803:1811	arg1	intestine					1820:1828	the intestine	1816:1828	the intestine of the piglets	1816:1843	Moreover, the intestinal morphology measured as villus height and crypt depths, and the inflammatory cytokines in the intestine of the piglets were not differentially expressed between maternal treatments.					
29969488	1	7	from	life	242:245	arg1	early					233:237	early	233:237	early	233:237	BACKGROUND Establishment of a beneficial microbiota profile for piglets as early in life as possible is important as it will impact their future health.					
29969488	3	8	theme	starch	691:696	arg1	starch					691:696	pea starch	687:696	pea starch	687:696	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	3	8	theme	starch	691:696	arg1	%					682:682	33%	680:682	33% of pea starch	680:696	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	1	9	theme	beneficial	188:197	arg1	profile					210:216	a beneficial microbiota profile	186:216	a beneficial microbiota profile for piglets as early in life	186:245	BACKGROUND Establishment of a beneficial microbiota profile for piglets as early in life as possible is important as it will impact their future health.					
29969488	4	10	theme	colonic	850:856	arg1	microbiota					858:867	colonic microbiota	850:867	colonic microbiota	850:867	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	10	11	theme	Only	1908:1911	arg1	occludens					1920:1928	Only zonula occludens 1	1908:1930	Only zonula occludens 1 (ZO-1)	1908:1937	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	10	11	theme	Only	1908:1911	arg1	ZO-1					1933:1936	ZO-1	1933:1936	ZO-1	1933:1936	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	6	12	theme	genera	1259:1264	arg1	abundance					1235:1243	the relative abundance	1222:1243	the relative abundance of beneficial genera like Bifidobacterium	1222:1285	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	6	12	theme	genera	1259:1264	arg1	ratio					1212:1216	Bacteroidetes ratio	1198:1216	Bacteroidetes ratio	1198:1216	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	1	13	theme	profile	210:216	arg1	Establishment					169:181	BACKGROUND Establishment	158:181	BACKGROUND Establishment of a beneficial microbiota profile for piglets as early in life as possible	158:257	BACKGROUND Establishment of a beneficial microbiota profile for piglets as early in life as possible is important as it will impact their future health.					
29969488	7	14	theme	RS	1584:1585	arg1	diet					1587:1590	the RS diet	1580:1590	the RS diet	1580:1590	Milk protein concentration decreased with RS diet and lactose concentration increased within the first weeks of lactation while decreased the week before weaning with the RS diet.					
29969488	10	15	theme	tight	2043:2047	arg1	junctions					2049:2057	the mucosa tight junctions	2032:2057	the mucosa tight junctions	2032:2057	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	6	16	theme	relative	1226:1233	arg1	abundance					1235:1243	the relative abundance	1222:1243	the relative abundance of beneficial genera like Bifidobacterium	1222:1285	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	2	17	theme	microbiota	525:534	arg1	composition					536:546	their milk and/or gut microbiota composition	503:546	composition	536:546	In the current study, we hypothesized that resistant starch (RS) provided in the maternal diet during gestation and lactation will be fermented in their hindgut, which would favourably modify their milk and/or gut microbiota composition and that it would in turn affect piglets' microbiota profile and their absorptive and immune abilities.					
29969488	3	18	used	used	702:705	arg2	%					682:682	33%	680:682	33% of pea starch	680:696	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	3	18	used	used	702:705	arg2	starch					691:696	pea starch	687:696	pea starch	687:696	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	1	19	from	early	233:237	arg1	life					242:245	life	242:245	life	242:245	BACKGROUND Establishment of a beneficial microbiota profile for piglets as early in life as possible is important as it will impact their future health.					
29969488	3	20	theme	control	767:773	arg1	sows					775:778	control sows	767:778	control sows	767:778	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	4	21	theme	piglets	953:959	arg1	parameters					935:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters	846:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets	846:959	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	10	22	theme	piglets	1974:1980	arg1	ileum					1965:1969	the ileum	1961:1969	the ileum of piglets born from RS sows	1961:1998	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	0	23	theme	faecal	75:80	arg1	microbiota					82:91	their faecal microbiota and milk composition	69:112	microbiota	82:91	Feeding sows resistant starch during gestation and lactation impacts their faecal microbiota and milk composition but shows limited effects on their progeny.					
29969488	5	24	theme	faecal	1057:1062	arg1	score					1064:1068	post-weaning faecal score	1044:1068	post-weaning faecal score	1044:1068	In addition, their overall performances and post-weaning faecal score were also assessed.					
29969488	1	25	theme	BACKGROUND	158:167	arg1	Establishment					169:181	BACKGROUND Establishment	158:181	BACKGROUND Establishment of a beneficial microbiota profile for piglets as early in life as possible	158:257	BACKGROUND Establishment of a beneficial microbiota profile for piglets as early in life as possible is important as it will impact their future health.					
29969488	4	26	theme	fatty	882:886	arg1	acids					888:892	short-chain fatty acids	870:892	short-chain fatty acids (SCFA) production	870:910	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	4	26	theme	fatty	882:886	arg1	SCFA					895:898	SCFA	895:898	SCFA	895:898	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	3	27	theme	sows	746:749	arg1	diet					714:717	the diet	710:717	the diet of gestating and lactating sows	710:749	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	7	28	theme	first	1510:1514	arg1	weeks					1516:1520	the first weeks	1506:1520	the first weeks of lactation	1506:1533	Milk protein concentration decreased with RS diet and lactose concentration increased within the first weeks of lactation while decreased the week before weaning with the RS diet.					
29969488	10	29	theme	mucosa	2036:2041	arg1	junctions					2049:2057	the mucosa tight junctions	2032:2057	the mucosa tight junctions	2032:2057	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	2	30	theme	microbiota	590:599	arg1	profile					601:607	piglets' microbiota profile	581:607	piglets' microbiota profile	581:607	In the current study, we hypothesized that resistant starch (RS) provided in the maternal diet during gestation and lactation will be fermented in their hindgut, which would favourably modify their milk and/or gut microbiota composition and that it would in turn affect piglets' microbiota profile and their absorptive and immune abilities.					
29969488	5	31	theme	overall	1019:1025	arg1	performances					1027:1038	their overall performances	1013:1038	their overall performances	1013:1038	In addition, their overall performances and post-weaning faecal score were also assessed.					
29969488	4	32	theme	health	920:925	arg1	parameters					935:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters	846:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets	846:959	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	2	33	theme	immune	634:639	arg1	abilities					641:649	their absorptive and immune abilities	613:649	their absorptive and immune abilities	613:649	In the current study, we hypothesized that resistant starch (RS) provided in the maternal diet during gestation and lactation will be fermented in their hindgut, which would favourably modify their milk and/or gut microbiota composition and that it would in turn affect piglets' microbiota profile and their absorptive and immune abilities.					
29969488	0	34	theme	Feeding	0:6	arg1	starch					23:28	Feeding sows resistant starch	0:28	Feeding sows resistant starch during gestation and lactation	0:59	Feeding sows resistant starch during gestation and lactation impacts their faecal microbiota and milk composition but shows limited effects on their progeny.					
29969488	2	35	theme	absorptive	619:628	arg1	abilities					641:649	their absorptive and immune abilities	613:649	their absorptive and immune abilities	613:649	In the current study, we hypothesized that resistant starch (RS) provided in the maternal diet during gestation and lactation will be fermented in their hindgut, which would favourably modify their milk and/or gut microbiota composition and that it would in turn affect piglets' microbiota profile and their absorptive and immune abilities.					
29969488	0	36	theme	resistant	13:21	arg1	starch					23:28	Feeding sows resistant starch	0:28	Feeding sows resistant starch during gestation and lactation	0:59	Feeding sows resistant starch during gestation and lactation impacts their faecal microbiota and milk composition but shows limited effects on their progeny.					
29969488	9	37	from	morphology	1727:1736	arg1	intestine					1820:1828	the intestine	1816:1828	the intestine of the piglets	1816:1843	Moreover, the intestinal morphology measured as villus height and crypt depths, and the inflammatory cytokines in the intestine of the piglets were not differentially expressed between maternal treatments.					
29969488	9	38	theme	crypt	1768:1772	arg1	depths					1774:1779	crypt depths	1768:1779	crypt depths	1768:1779	Moreover, the intestinal morphology measured as villus height and crypt depths, and the inflammatory cytokines in the intestine of the piglets were not differentially expressed between maternal treatments.					
29969488	7	39	theme	lactose	1467:1473	arg1	concentration					1475:1487	lactose concentration	1467:1487	lactose concentration	1467:1487	Milk protein concentration decreased with RS diet and lactose concentration increased within the first weeks of lactation while decreased the week before weaning with the RS diet.					
29969488	6	40	theme	maternal	1342:1349	arg1	diets					1351:1355	maternal diets	1342:1355	maternal diets	1342:1355	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	11	41	theme	milk	2211:2214	arg1	composition					2216:2226	milk composition	2211:2226	milk composition	2211:2226	CONCLUSION Changes in the microbiota transferred from mother to piglets due to the inclusion of RS in the maternal diet are rather limited even though milk composition was affected.					
29969488	0	42	from	effects	132:138	arg1	progeny					149:155	their progeny	143:155	their progeny	143:155	Feeding sows resistant starch during gestation and lactation impacts their faecal microbiota and milk composition but shows limited effects on their progeny.					
29969488	3	43	theme	gestating	722:730	arg1	sows					746:749	gestating and lactating sows	722:749	gestating and lactating sows	722:749	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	10	44	theme	better	2014:2019	arg1	closure					2021:2027	a better closure	2012:2027	a better closure of the mucosa tight junctions	2012:2057	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	6	45	theme	progeny	1404:1410	arg1	microbiota					1384:1393	the colonic microbiota	1372:1393	the colonic microbiota of their progeny	1372:1410	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	7	46	theme	Milk	1413:1416	arg1	concentration					1426:1438	Milk protein concentration	1413:1438	Milk protein concentration	1413:1438	Milk protein concentration decreased with RS diet and lactose concentration increased within the first weeks of lactation while decreased the week before weaning with the RS diet.					
29969488	4	47	theme	faecal	787:792	arg1	microbiota					794:803	Their faecal microbiota	781:803	Their faecal microbiota	781:803	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	6	48	theme	RS	1102:1103	arg1	diet					1105:1108	The RS diet	1098:1108	The RS diet	1098:1108	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	9	49	theme	piglets	1837:1843	arg1	intestine					1820:1828	the intestine	1816:1828	the intestine of the piglets	1816:1843	Moreover, the intestinal morphology measured as villus height and crypt depths, and the inflammatory cytokines in the intestine of the piglets were not differentially expressed between maternal treatments.					
29969488	11	50	theme	maternal	2166:2173	arg1	diet					2175:2178	the maternal diet	2162:2178	the maternal diet	2162:2178	CONCLUSION Changes in the microbiota transferred from mother to piglets due to the inclusion of RS in the maternal diet are rather limited even though milk composition was affected.					
29969488	11	51	theme	CONCLUSION	2060:2069	arg1	Changes					2071:2077	CONCLUSION Changes	2060:2077	CONCLUSION Changes in the microbiota transferred from mother to piglets due to the inclusion of RS in the maternal diet	2060:2178	CONCLUSION Changes in the microbiota transferred from mother to piglets due to the inclusion of RS in the maternal diet are rather limited even though milk composition was affected.					
29969488	10	52	theme	zonula	1913:1918	arg1	occludens					1920:1928	Only zonula occludens 1	1908:1930	Only zonula occludens 1 (ZO-1)	1908:1937	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	10	52	theme	zonula	1913:1918	arg1	ZO-1					1933:1936	ZO-1	1933:1936	ZO-1	1933:1936	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	0	53	theme	limited	124:130	arg1	effects					132:138	limited effects	124:138	limited effects on their progeny	124:155	Feeding sows resistant starch during gestation and lactation impacts their faecal microbiota and milk composition but shows limited effects on their progeny.					
29969488	8	54	theme	diarrhoea	1655:1663	arg1	frequency					1665:1673	diarrhoea frequency post-weaning	1655:1686	diarrhoea frequency post-weaning	1655:1686	No effect of the dietary treatment, on piglets' bodyweight or diarrhoea frequency post-weaning was observed.					
29969488	1	55	theme	microbiota	199:208	arg1	profile					210:216	a beneficial microbiota profile	186:216	a beneficial microbiota profile for piglets as early in life	186:245	BACKGROUND Establishment of a beneficial microbiota profile for piglets as early in life as possible is important as it will impact their future health.					
29969488	10	56	theme	junctions	2049:2057	arg1	closure					2021:2027	a better closure	2012:2027	a better closure of the mucosa tight junctions	2012:2057	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	6	57	dep	RESULTS	1090:1096	arg1	modulated					1110:1118	modulated	1110:1118	modulated	1110:1118	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	3	58	theme	pea	687:689	arg1	starch					691:696	pea starch	687:696	pea starch	687:696	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	6	59	theme	beneficial	1248:1257	arg1	genera					1259:1264	beneficial genera	1248:1264	beneficial genera like Bifidobacterium	1248:1285	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	8	60	theme	post-weaning	1675:1686	arg1	frequency					1665:1673	diarrhoea frequency post-weaning	1655:1686	diarrhoea frequency post-weaning	1655:1686	No effect of the dietary treatment, on piglets' bodyweight or diarrhoea frequency post-weaning was observed.					
29969488	4	61	theme	microbiota	858:867	arg1	parameters					935:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters	846:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets	846:959	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	6	62	theme	sows	1149:1152	arg1	microbiota					1131:1140	the faecal microbiota	1120:1140	the faecal microbiota of the sows during gestation	1120:1169	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	11	63	theme	RS	2156:2157	arg1	inclusion					2143:2151	the inclusion	2139:2151	the inclusion of RS in the maternal diet	2139:2178	CONCLUSION Changes in the microbiota transferred from mother to piglets due to the inclusion of RS in the maternal diet are rather limited even though milk composition was affected.					
29969488	2	64	theme	gut	521:523	arg1	composition					536:546	their milk and/or gut microbiota composition	503:546	composition	536:546	In the current study, we hypothesized that resistant starch (RS) provided in the maternal diet during gestation and lactation will be fermented in their hindgut, which would favourably modify their milk and/or gut microbiota composition and that it would in turn affect piglets' microbiota profile and their absorptive and immune abilities.					
29969488	6	65	theme	faecal	1124:1129	arg1	microbiota					1131:1140	the faecal microbiota	1120:1140	the faecal microbiota of the sows during gestation	1120:1169	RESULTS The RS diet modulated the faecal microbiota of the sows during gestation, increasing the Firmicutes:Bacteroidetes ratio and the relative abundance of beneficial genera like Bifidobacterium but these differences disappeared during lactation and maternal diets did not impact the colonic microbiota of their progeny.					
29969488	3	66	theme	lactating	736:744	arg1	sows					746:749	gestating and lactating sows	722:749	gestating and lactating sows	722:749	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	10	67	theme	RS	1992:1993	arg1	sows					1995:1998	RS sows	1992:1998	RS sows	1992:1998	Only zonula occludens 1 (ZO-1) was more expressed in the ileum of piglets born from RS sows, suggesting a better closure of the mucosa tight junctions.					
29969488	7	68	theme	lactation	1525:1533	arg1	weeks					1516:1520	the first weeks	1506:1520	the first weeks of lactation	1506:1533	Milk protein concentration decreased with RS diet and lactose concentration increased within the first weeks of lactation while decreased the week before weaning with the RS diet.					
29969488	9	69	theme	intestinal	1716:1725	arg1	morphology					1727:1736	the intestinal morphology	1712:1736	the intestinal morphology measured as villus height and crypt depths	1712:1779	Moreover, the intestinal morphology measured as villus height and crypt depths, and the inflammatory cytokines in the intestine of the piglets were not differentially expressed between maternal treatments.					
29969488	5	70	theme	post-weaning	1044:1055	arg1	score					1064:1068	post-weaning faecal score	1044:1068	post-weaning faecal score	1044:1068	In addition, their overall performances and post-weaning faecal score were also assessed.					
29969488	4	71	theme	production	901:910	arg1	parameters					935:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters	846:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets	846:959	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	0	72	theme	milk	97:100	arg1	composition					102:112	their faecal microbiota and milk composition	69:112	composition	102:112	Feeding sows resistant starch during gestation and lactation impacts their faecal microbiota and milk composition but shows limited effects on their progeny.					
29969488	4	73	dep	days	979:982	arg1	weaning					991:997	weaning	991:997	weaning	991:997	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	4	74	theme	short-chain	870:880	arg1	acids					888:892	short-chain fatty acids	870:892	short-chain fatty acids (SCFA) production	870:910	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	4	74	theme	short-chain	870:880	arg1	SCFA					895:898	SCFA	895:898	SCFA	895:898	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	9	75	theme	maternal	1887:1894	arg1	treatments					1896:1905	maternal treatments	1887:1905	maternal treatments	1887:1905	Moreover, the intestinal morphology measured as villus height and crypt depths, and the inflammatory cytokines in the intestine of the piglets were not differentially expressed between maternal treatments.					
29969488	4	76	theme	acids	888:892	arg1	production					901:910	short-chain fatty acids (SCFA) production	870:910	short-chain fatty acids (SCFA) production	870:910	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	0	77	theme	sows	8:11	arg1	starch					23:28	Feeding sows resistant starch	0:28	Feeding sows resistant starch during gestation and lactation	0:59	Feeding sows resistant starch during gestation and lactation impacts their faecal microbiota and milk composition but shows limited effects on their progeny.					
29969488	9	78	theme	villus	1750:1755	arg1	height					1757:1762	villus height	1750:1762	villus height	1750:1762	Moreover, the intestinal morphology measured as villus height and crypt depths, and the inflammatory cytokines in the intestine of the piglets were not differentially expressed between maternal treatments.					
29969488	2	79	theme	resistant	354:362	arg1	RS					372:373	RS	372:373	RS	372:373	In the current study, we hypothesized that resistant starch (RS) provided in the maternal diet during gestation and lactation will be fermented in their hindgut, which would favourably modify their milk and/or gut microbiota composition and that it would in turn affect piglets' microbiota profile and their absorptive and immune abilities.					
29969488	2	79	theme	resistant	354:362	arg1	starch					364:369	resistant starch	354:369	resistant starch (RS) provided in the maternal diet during gestation and lactation	354:435	In the current study, we hypothesized that resistant starch (RS) provided in the maternal diet during gestation and lactation will be fermented in their hindgut, which would favourably modify their milk and/or gut microbiota composition and that it would in turn affect piglets' microbiota profile and their absorptive and immune abilities.					
29969488	3	80	dep	METHODS	652:658	arg1	compared					755:762	compared	755:762	compared to control sows	755:778	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	3	80	dep	METHODS	652:658	arg1	used					702:705	used	702:705	used in the diet of gestating and lactating sows	702:749	METHODS In this experiment, 33% of pea starch was used in the diet of gestating and lactating sows and compared to control sows.					
29969488	2	81	theme	maternal	392:399	arg1	diet					401:404	the maternal diet	388:404	the maternal diet	388:404	In the current study, we hypothesized that resistant starch (RS) provided in the maternal diet during gestation and lactation will be fermented in their hindgut, which would favourably modify their milk and/or gut microbiota composition and that it would in turn affect piglets' microbiota profile and their absorptive and immune abilities.					
29969488	11	82	from	inclusion	2143:2151	arg1	diet					2175:2178	the maternal diet	2162:2178	the maternal diet	2162:2178	CONCLUSION Changes in the microbiota transferred from mother to piglets due to the inclusion of RS in the maternal diet are rather limited even though milk composition was affected.					
29969488	4	83	theme	gut	916:918	arg1	health					920:925	gut health	916:925	gut health	916:925	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	1	84	theme	future	296:301	arg1	health					303:308	their future health	290:308	their future health	290:308	BACKGROUND Establishment of a beneficial microbiota profile for piglets as early in life as possible is important as it will impact their future health.					
29969488	4	85	theme	related	927:933	arg1	parameters					935:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters	846:944	the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets	846:959	Their faecal microbiota and milk composition were determined and the colonic microbiota, short-chain fatty acids (SCFA) production and gut health related parameters of the piglets were measured two days before weaning.					
29969488	2	86	theme	current	318:324	arg1	study					326:330	the current study	314:330	the current study	314:330	In the current study, we hypothesized that resistant starch (RS) provided in the maternal diet during gestation and lactation will be fermented in their hindgut, which would favourably modify their milk and/or gut microbiota composition and that it would in turn affect piglets' microbiota profile and their absorptive and immune abilities.					
29969488	9	87	theme	inflammatory	1790:1801	arg1	cytokines					1803:1811	the inflammatory cytokines	1786:1811	the inflammatory cytokines in the intestine of the piglets	1786:1843	Moreover, the intestinal morphology measured as villus height and crypt depths, and the inflammatory cytokines in the intestine of the piglets were not differentially expressed between maternal treatments.					
29969488	7	88	theme	RS	1455:1456	arg1	diet					1458:1461	RS diet	1455:1461	RS diet	1455:1461	Milk protein concentration decreased with RS diet and lactose concentration increased within the first weeks of lactation while decreased the week before weaning with the RS diet.					
29969488	8	89	theme	treatment	1618:1626	arg1	effect					1596:1601	No effect	1593:1601	No effect	1593:1601	No effect of the dietary treatment, on piglets' bodyweight or diarrhoea frequency post-weaning was observed.					
31883900	6	0	theme	saturated	988:996	arg1	%					1018:1018	55%	1016:1018	55%	1016:1018	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	0	theme	saturated	988:996	arg1	RESULTS					976:982	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde	976:1063	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C	976:1080	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	0	theme	saturated	988:996	arg1	sulfate					1007:1013	The saturated ammonium sulfate	984:1013	The saturated ammonium sulfate (55%)	984:1019	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	8	1	from	applications	1602:1613	arg1	improvement					1547:1557	the improvement	1543:1557	the improvement of bio catalytic systems in the industrial applications	1543:1613	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	3	2	theme	current	537:543	arg1	study					545:549	The current study	533:549	The current study	533:549	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	2	3	theme	expensive	514:522	arg1	method					507:512	this assay method expensive	496:522	this assay method expensive	496:522	The recovery of soluble lipase is not possible which makes this assay method expensive.					
31883900	0	4	from	immobilization	7:20	arg1	composites					112:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites	25:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts	25:141	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	1	5	theme	BACKGROUND	191:200	arg1	Lipase					202:207	BACKGROUND Lipase	191:207	BACKGROUND Lipase	191:207	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	1	5	theme	BACKGROUND	191:200	arg1	enzyme					215:220	an enzyme	212:220	an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids	212:434	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	1	6	theme	organic	276:282	arg1	interactions					284:295	watery and organic interactions	265:295	watery and organic interactions	265:295	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	6	7	theme	%	1189:1189	arg1	recovery					1173:1180	the maximum activity recovery	1152:1180	the maximum activity recovery of 96.5%	1152:1189	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	8	link	cross-linked	1025:1036	arg1	glutaraldehyde					1050:1063	cross-linked with 125 mM glutaraldehyde	1025:1063	cross-linked with 125 mM glutaraldehyde	1025:1063	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	8	link	cross-linked	1025:1036	arg1	RESULTS					976:982	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde	976:1063	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C	976:1080	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	0	9	theme	new	126:128	arg1	biocatalysts					130:141	new biocatalysts	126:141	new biocatalysts	126:141	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	8	10	theme	bio	1562:1564	arg1	systems					1576:1582	bio catalytic systems	1562:1582	bio catalytic systems in the industrial applications	1562:1613	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	4	11	theme	lipase-GO-MNPs-CLEAs	874:893	arg1	activities					895:904	the free lipase and lipase-GO-MNPs-CLEAs activities	854:904	activities	895:904	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	7	12	theme	free	1280:1283	arg1	lipase					1285:1290	the free lipase	1276:1290	the free lipase	1276:1290	Storage stability significantly developed for the lipase-GO-MNPs-CLEAs comparing to the free lipase.					
31883900	8	13	from	performance	1441:1451	arg1	conditions					1456:1465	conditions	1456:1465	conditions	1456:1465	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	6	14	theme	lipase-GO-MNPs-CLEAs	1126:1145	arg1	synthesis					1113:1121	the synthesis	1109:1121	the synthesis of lipase-GO-MNPs-CLEAs	1109:1145	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	0	15	theme	Improved	144:151	arg1	reusability					153:163	Improved reusability	144:163	Improved reusability	144:163	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	6	16	with	optimization	1096:1107	arg1	recovery					1173:1180	the maximum activity recovery	1152:1180	the maximum activity recovery of 96.5%	1152:1189	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	3	17	dep	METHODS	525:531	arg1	efforts					551:557	efforts	551:557	efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs)	551:668	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	4	18	used	used	838:841	arg2	reusability					799:809	reusability	799:809	reusability	799:809	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	18	used	used	838:841	arg2	temperature					763:773	temperature	763:773	temperature	763:773	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	18	used	used	838:841	arg2	conditions					744:753	different conditions	734:753	different conditions such as temperature, pH, storage stability, reusability, and organic solvents	734:831	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	18	used	used	838:841	arg2	pH					776:777	pH	776:777	pH	776:777	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	18	used	used	838:841	arg2	stability					788:796	storage stability	780:796	storage stability	780:796	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	18	used	used	838:841	arg2	solvents					824:831	organic solvents	816:831	organic solvents	816:831	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	19	theme	graphene	680:687	arg1	GO-MNPs					710:716	GO-MNPs	710:716	GO-MNPs	710:716	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	19	theme	graphene	680:687	arg1	nanoparticles					695:707	Then the graphene oxide nanoparticles	671:707	Then the graphene oxide nanoparticles (GO-MNPs)	671:717	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	8	20	theme	systems	1576:1582	arg1	improvement					1547:1557	the improvement	1543:1557	the improvement of bio catalytic systems in the industrial applications	1543:1613	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	8	21	from	systems	1576:1582	arg1	applications					1602:1613	the industrial applications	1587:1613	the industrial applications	1587:1613	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	8	22	theme	first	1331:1335	arg1	time					1337:1340	the first time	1327:1340	the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications	1327:1613	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	4	23	theme	storage	780:786	arg1	stability					788:796	storage stability	780:796	storage stability	780:796	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	7	24	theme	Storage	1192:1198	arg1	stability					1200:1208	Storage stability	1192:1208	Storage stability	1192:1208	Storage stability significantly developed for the lipase-GO-MNPs-CLEAs comparing to the free lipase.					
31883900	8	25	theme	catalytic	1566:1574	arg1	systems					1576:1582	bio catalytic systems	1562:1582	bio catalytic systems in the industrial applications	1562:1613	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	4	26	theme	free	858:861	arg1	lipase					863:868	the free lipase and lipase-GO-MNPs-CLEAs activities	854:904	lipase	863:868	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	5	27	theme	preparation	953:963	arg1	compounds					965:973	the preparation compounds	949:973	the preparation compounds	949:973	Different techniques were used to analyze the preparation compounds.					
31883900	8	28	from	stability	1409:1417	arg1	conditions					1456:1465	conditions	1456:1465	conditions	1456:1465	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	0	29	theme	Lipase	0:5	arg1	immobilization					7:20	Lipase immobilization	0:20	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.	0:189	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	3	30	theme	lipase	600:605	arg1	antarctica					617:626	a lipase B Candida antarctica	598:626	a lipase B Candida antarctica	598:626	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	4	31	theme	oxide	689:693	arg1	GO-MNPs					710:716	GO-MNPs	710:716	GO-MNPs	710:716	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	31	theme	oxide	689:693	arg1	nanoparticles					695:707	Then the graphene oxide nanoparticles	671:707	Then the graphene oxide nanoparticles (GO-MNPs)	671:717	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	0	32	theme	synthesized	25:35	arg1	composites					112:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites	25:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts	25:141	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	3	33	theme	Cross-Linked	631:642	arg1	CLEAs					663:667	CLEAs	663:667	CLEAs	663:667	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	3	33	theme	Cross-Linked	631:642	arg1	Aggregates					651:660	Cross-Linked Enzyme Aggregates	631:660	Cross-Linked Enzyme Aggregates (CLEAs)	631:668	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	1	34	from	interactions	284:295	arg1	hydrolysis					241:250	the hydrolysis	237:250	the hydrolysis of lipids in watery and organic interactions	237:295	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	8	35	theme	nano-biocatalytic	1423:1439	arg1	performance					1441:1451	nano-biocatalytic performance	1423:1451	nano-biocatalytic performance	1423:1451	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	3	36	theme	stability	585:593	arg1	development					566:576	the development	562:576	the development of the stability of a lipase B Candida antarctica	562:626	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	6	37	with	cross-linked	1025:1036	arg1	125 mM					1043:1048	125 mM	1043:1048	125 mM	1043:1048	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	2	38	theme	lipase	461:466	arg1	possible					475:482	possible	475:482	possible	475:482	The recovery of soluble lipase is not possible which makes this assay method expensive.					
31883900	2	38	theme	lipase	461:466	arg1	recovery					441:448	The recovery	437:448	The recovery of soluble lipase	437:466	The recovery of soluble lipase is not possible which makes this assay method expensive.					
31883900	8	39	from	improvement	1547:1557	arg1	applications					1602:1613	the industrial applications	1587:1613	the industrial applications	1587:1613	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	1	40	theme	synthetic	315:323	arg1	reactions					325:333	synthetic reactions	315:333	synthetic reactions	315:333	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	1	41	theme	fatty	424:428	arg1	acids					430:434	fatty acids	424:434	fatty acids	424:434	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	6	42	theme	activity	1164:1171	arg1	recovery					1173:1180	the maximum activity recovery	1152:1180	the maximum activity recovery of 96.5%	1152:1189	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	2	43	theme	soluble	453:459	arg1	lipase					461:466	soluble lipase	453:466	soluble lipase	453:466	The recovery of soluble lipase is not possible which makes this assay method expensive.					
31883900	0	44	theme	acid-coated	48:58	arg1	composites					112:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites	25:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts	25:141	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	8	45	theme	industrial	1591:1600	arg1	applications					1602:1613	the industrial applications	1587:1613	the industrial applications	1587:1613	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	3	46	theme	antarctica	617:626	arg1	stability					585:593	the stability	581:593	the stability of a lipase B Candida antarctica	581:626	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	0	47	theme	hyaluronic	37:46	arg1	composites					112:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites	25:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts	25:141	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	8	48	dep	stability	1409:1417	arg1	the					1405:1407	the	1405:1407	the	1405:1407	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	2	49	theme	assay	501:505	arg1	method					507:512	this assay method expensive	496:522	this assay method expensive	496:522	The recovery of soluble lipase is not possible which makes this assay method expensive.					
31883900	6	50	dep	RESULTS	976:982	arg1	%					1018:1018	55%	1016:1018	55%	1016:1018	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	50	dep	RESULTS	976:982	arg1	glutaraldehyde					1050:1063	cross-linked with 125 mM glutaraldehyde	1025:1063	cross-linked with 125 mM glutaraldehyde	1025:1063	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	50	dep	RESULTS	976:982	arg1	RESULTS					976:982	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde	976:1063	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C	976:1080	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	50	dep	RESULTS	976:982	arg1	sulfate					1007:1013	The saturated ammonium sulfate	984:1013	The saturated ammonium sulfate (55%)	984:1019	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	4	51	theme	organic	816:822	arg1	solvents					824:831	organic solvents	816:831	organic solvents	816:831	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	8	52	dep	CONCLUSIONS	1293:1303	arg1	study					1317:1321	The current study	1305:1321	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.	1293:1614	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	0	53	theme	nanoparticle-functionalized	69:95	arg1	composites					112:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites	25:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts	25:141	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	6	54	theme	ammonium	998:1005	arg1	%					1018:1018	55%	1016:1018	55%	1016:1018	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	54	theme	ammonium	998:1005	arg1	RESULTS					976:982	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde	976:1063	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C	976:1080	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	54	theme	ammonium	998:1005	arg1	sulfate					1007:1013	The saturated ammonium sulfate	984:1013	The saturated ammonium sulfate (55%)	984:1019	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	3	55	theme	B	607:607	arg1	antarctica					617:626	a lipase B Candida antarctica	598:626	a lipase B Candida antarctica	598:626	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	1	56	from	hydrolysis	241:250	arg1	interactions					284:295	watery and organic interactions	265:295	watery and organic interactions	265:295	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	6	57	theme	maximum	1156:1162	arg1	recovery					1173:1180	the maximum activity recovery	1152:1180	the maximum activity recovery of 96.5%	1152:1189	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	0	58	theme	magnetic	60:67	arg1	composites					112:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites	25:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts	25:141	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	1	59	theme	inter	346:350	arg1	esterification					352:365	inter esterification	346:365	inter esterification among triglycerides and alcohols to produce glycerin and fatty acids	346:434	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	8	60	dep	solvent	1476:1482	arg1	the					1472:1474	the	1472:1474	the	1472:1474	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	3	61	theme	Candida	609:615	arg1	antarctica					617:626	a lipase B Candida antarctica	598:626	a lipase B Candida antarctica	598:626	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	3	62	theme	Enzyme	644:649	arg1	CLEAs					663:667	CLEAs	663:667	CLEAs	663:667	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	3	62	theme	Enzyme	644:649	arg1	Aggregates					651:660	Cross-Linked Enzyme Aggregates	631:660	Cross-Linked Enzyme Aggregates (CLEAs)	631:668	METHODS The current study efforts on the development of the stability of a lipase B Candida antarctica as Cross-Linked Enzyme Aggregates (CLEAs).					
31883900	6	63	from	4 °C	1077:1080	arg1	10 h					1069:1072	10 h	1069:1072	10 h at 4 °C	1069:1080	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	1	64	from	lipids	255:260	arg1	interactions					284:295	watery and organic interactions	265:295	watery and organic interactions	265:295	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	8	65	theme	significant	1518:1528	arg1	influence					1530:1538	a significant influence	1516:1538	a significant influence	1516:1538	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	4	66	theme	different	734:742	arg1	pH					776:777	pH	776:777	pH	776:777	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	66	theme	different	734:742	arg1	conditions					744:753	different conditions	734:753	different conditions such as temperature, pH, storage stability, reusability, and organic solvents	734:831	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	66	theme	different	734:742	arg1	stability					788:796	storage stability	780:796	storage stability	780:796	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	66	theme	different	734:742	arg1	solvents					824:831	organic solvents	816:831	organic solvents	816:831	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	66	theme	different	734:742	arg1	temperature					763:773	temperature	763:773	temperature	763:773	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	4	66	theme	different	734:742	arg1	reusability					799:809	reusability	799:809	reusability	799:809	Then the graphene oxide nanoparticles (GO-MNPs) were prepared, different conditions such as temperature, pH, storage stability, reusability, and organic solvents were used to compare the free lipase and lipase-GO-MNPs-CLEAs activities.					
31883900	8	67	theme	hyaluronic	1369:1378	arg1	HA					1386:1387	HA	1386:1387	HA	1386:1387	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	8	67	theme	hyaluronic	1369:1378	arg1	acid					1380:1383	hyaluronic acid	1369:1383	hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications	1369:1613	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	1	68	theme	lipids	255:260	arg1	hydrolysis					241:250	the hydrolysis	237:250	the hydrolysis of lipids in watery and organic interactions	237:295	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	0	69	theme	oxide	106:110	arg1	composites					112:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites	25:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts	25:141	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	5	70	used	used	933:936	arg2	techniques					917:926	Different techniques	907:926	Different techniques	907:926	Different techniques were used to analyze the preparation compounds.					
31883900	0	71	dep	immobilization	7:20	arg1	stability					166:174	stability	166:174	stability	166:174	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	0	71	dep	immobilization	7:20	arg1	activity					181:188	activity	181:188	activity	181:188	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	0	71	dep	immobilization	7:20	arg1	reusability					153:163	Improved reusability	144:163	Improved reusability	144:163	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	8	72	theme	current	1309:1315	arg1	study					1317:1321	The current study	1305:1321	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.	1293:1614	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	8	73	contain	have	1511:1514	arg1	temperature					1488:1498	temperature	1488:1498	temperature	1488:1498	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	8	73	contain	have	1511:1514	arg2	influence					1530:1538	a significant influence	1516:1538	a significant influence	1516:1538	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	8	73	contain	have	1511:1514	arg1	solvent					1476:1482	solvent	1476:1482	solvent	1476:1482	CONCLUSIONS The current study for the first time modified the MNPs by using hyaluronic acid (HA) which improved the stability and nano-biocatalytic performance in conditions into the solvent and temperature which will have a significant influence on the improvement of bio catalytic systems in the industrial applications.					
31883900	6	74	theme	cross-linked	1025:1036	arg1	glutaraldehyde					1050:1063	cross-linked with 125 mM glutaraldehyde	1025:1063	cross-linked with 125 mM glutaraldehyde	1025:1063	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	6	74	theme	cross-linked	1025:1036	arg1	RESULTS					976:982	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde	976:1063	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C	976:1080	RESULTS The saturated ammonium sulfate (55%) and cross-linked with 125 mM glutaraldehyde for 10 h at 4 °C were found to optimization the synthesis of lipase-GO-MNPs-CLEAs with the maximum activity recovery of 96.5%.					
31883900	0	75	theme	graphene	97:104	arg1	composites					112:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites	25:121	synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts	25:141	Lipase immobilization on synthesized hyaluronic acid-coated magnetic nanoparticle-functionalized graphene oxide composites as new biocatalysts: Improved reusability, stability, and activity.					
31883900	1	76	dep	catalyzes	227:235	arg1	catalyze					306:313	catalyze	306:313	catalyze	306:313	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
31883900	5	77	theme	Different	907:915	arg1	techniques					917:926	Different techniques	907:926	Different techniques	907:926	Different techniques were used to analyze the preparation compounds.					
31883900	1	78	theme	watery	265:270	arg1	interactions					284:295	watery and organic interactions	265:295	watery and organic interactions	265:295	BACKGROUND Lipase is an enzyme that catalyzes the hydrolysis of lipids in watery and organic interactions they can catalyze synthetic reactions, involving inter esterification among triglycerides and alcohols to produce glycerin and fatty acids.					
30033312	6	0	theme	zeta	1115:1118	arg1	analysis					1127:1134	zeta seizer analysis	1115:1134	zeta seizer analysis for the same concentration of drug and stabilizer	1115:1184	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	6	1	theme	nanoparticles	1195:1207	arg1	size					1187:1190	size	1187:1190	size of nanoparticles	1187:1207	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	4	2	theme	polymer	846:852	arg1	nanoparticles					885:897	polymer stabilized molecular composite nanoparticles	846:897	polymer stabilized molecular composite nanoparticles	846:897	When compared with pure drug nanocrystals, polymer stabilized molecular composite nanoparticles were of smaller size and spherical shape.					
30033312	10	3	theme	NGP	1702:1704	arg1	formulations					1686:1697	optimized formulations	1676:1697	optimized formulations of NGP (N9)	1676:1709	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	3	4	theme	polymer	718:724	arg1	concentration					692:704	concentration	692:704	concentration of drug and polymer	692:724	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	4	5	theme	molecular	865:873	arg1	nanoparticles					885:897	polymer stabilized molecular composite nanoparticles	846:897	polymer stabilized molecular composite nanoparticles	846:897	When compared with pure drug nanocrystals, polymer stabilized molecular composite nanoparticles were of smaller size and spherical shape.					
30033312	6	6	theme	stabilizer	1175:1184	arg1	concentration					1149:1161	the same concentration	1140:1161	the same concentration of drug and stabilizer	1140:1184	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	6	7	theme	reverse	1263:1269	arg1	case					1271:1274	reverse case	1263:1274	reverse case	1263:1274	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	11	8	dep	polymer	1956:1962	arg1	stabilized					1964:1973	stabilized	1964:1973	stabilized	1964:1973	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
30033312	6	9	theme	drug	1166:1169	arg1	concentration					1149:1161	the same concentration	1140:1161	the same concentration of drug and stabilizer	1140:1184	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	3	10	theme	response	568:575	arg1	methodology					585:595	response surface methodology	568:595	response surface methodology based on 32 factorial design	568:624	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	10	11	theme	optimized	1676:1684	arg1	formulations					1686:1697	optimized formulations	1676:1697	optimized formulations of NGP (N9)	1676:1709	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	3	12	theme	dependent	781:789	arg1	nanoparticles					766:778	nanoparticles	766:778	nanoparticles (dependent variables)	766:800	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	3	12	theme	dependent	781:789	arg1	variables					791:799	dependent variables	781:799	dependent variables	781:799	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	0	13	theme	neem	139:142	arg1	gum					144:146	neem gum	139:146	neem gum	139:146	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	10	14	theme	1°	1808:1809	arg1	order					1811:1815	1° order	1808:1815	1° order	1808:1815	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	11	15	from	pattern	1919:1925	arg1	nanoparticles					1975:1987	three different polymer stabilized nanoparticles	1940:1987	three different polymer stabilized nanoparticles	1940:1987	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
30033312	3	16	theme	nanoparticles	766:778	arg1	efficiency					752:761	entrapment efficiency	741:761	entrapment efficiency	741:761	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	3	16	theme	nanoparticles	766:778	arg1	size					732:735	size	732:735	size	732:735	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	4	17	theme	spherical	924:932	arg1	shape					934:938	spherical shape	924:938	spherical shape	924:938	When compared with pure drug nanocrystals, polymer stabilized molecular composite nanoparticles were of smaller size and spherical shape.					
30033312	0	18	theme	neem	170:173	arg1	gum					175:177	carboxymethylated neem gum	152:177	carboxymethylated neem gum	152:177	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	9	19	theme	nanoparticles	1545:1557	arg1	non-aggregation					1526:1540	non-aggregation	1526:1540	non-aggregation of nanoparticles	1526:1557	SEM revealed spherical shaped and non-aggregation of nanoparticles.					
30033312	7	20	theme	CMNGP	1379:1383	arg1	hydrophilicity					1361:1374	better hydrophilicity	1354:1374	better hydrophilicity of CMNGP than NGP followed by NGP-g-Am	1354:1413	Contact angle determination easily predict better hydrophilicity of CMNGP than NGP followed by NGP-g-Am.					
30033312	0	21	theme	solvent-antisolvent	72:90	arg1	acrylamide					120:129	acrylamide	120:129	acrylamide	120:129	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	0	21	theme	solvent-antisolvent	72:90	arg1	method					92:97	ultrasound assisted solvent-antisolvent method	52:97	ultrasound assisted solvent-antisolvent method	52:97	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	10	22	dep	nanosuspension	1569:1582	arg1	show					1584:1587	show	1584:1587	show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1)	1584:1739	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	2	23	theme	drug	528:531	arg1	nanoprecipitates					533:548	drug nanoprecipitates	528:548	drug nanoprecipitates	528:548	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	24	theme	acrylamide	415:424	arg1	copolymer					432:440	acrylamide graft copolymer	415:440	acrylamide graft copolymer of neem gum	415:452	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	0	25	theme	gum	115:117	arg1	Effect					100:105	Effect	100:105	Effect of neem gum	100:117	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	11	26	theme	factor	1882:1887	arg1	analysis					1889:1896	Similarity factor analysis	1871:1896	Similarity factor analysis	1871:1896	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
30033312	0	27	theme	Stability	0:8	arg1	facilitation					10:21	Stability facilitation	0:21	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide	0:129	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	7	28	theme	better	1354:1359	arg1	hydrophilicity					1361:1374	better hydrophilicity	1354:1374	better hydrophilicity of CMNGP than NGP followed by NGP-g-Am	1354:1413	Contact angle determination easily predict better hydrophilicity of CMNGP than NGP followed by NGP-g-Am.					
30033312	8	29	theme	DSC	1416:1418	arg1	thermogram					1420:1429	DSC thermogram	1416:1429	DSC thermogram	1416:1429	DSC thermogram predict the amorphous nature of CMNGP than NGP and NGP-g-Am.					
30033312	2	30	theme	neem	445:448	arg1	gum					450:452	neem gum	445:452	neem gum	445:452	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	1	31	theme	developed	291:299	arg1	method					346:351	newly developed ultrasonication assisted solvent-antisolvent method	285:351	newly developed ultrasonication assisted solvent-antisolvent method	285:351	In present research, etoricoxib nanoparticles were prepared using newly developed ultrasonication assisted solvent-antisolvent method.					
30033312	5	32	theme	smaller	988:994	arg1	size					996:999	smaller size	988:999	smaller size hence	988:1005	CMNGP stabilized composite nanoparticles shown smaller size hence better performance in terms of solubility and dissolution rate as compared to NGP and NGP-g-Am.					
30033312	6	33	located	found	1214:1218	arg2	size					1187:1190	size	1187:1190	size of nanoparticles	1187:1207	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	6	33	located	found	1214:1218	arg1	range					1227:1231	the range	1223:1231	the range of NGP-g-Am > NGP > CMNGP	1223:1257	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	4	34	theme	pure	822:825	arg1	nanocrystals					832:843	pure drug nanocrystals	822:843	pure drug nanocrystals	822:843	When compared with pure drug nanocrystals, polymer stabilized molecular composite nanoparticles were of smaller size and spherical shape.					
30033312	11	35	theme	release	1911:1917	arg1	pattern					1919:1925	release pattern	1911:1925	release pattern of drug from three different polymer stabilized nanoparticles	1911:1987	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
30033312	1	36	theme	assisted	317:324	arg1	method					346:351	newly developed ultrasonication assisted solvent-antisolvent method	285:351	newly developed ultrasonication assisted solvent-antisolvent method	285:351	In present research, etoricoxib nanoparticles were prepared using newly developed ultrasonication assisted solvent-antisolvent method.					
30033312	5	37	theme	dissolution	1053:1063	arg1	terms					1029:1033	terms	1029:1033	terms of solubility and dissolution rate	1029:1068	CMNGP stabilized composite nanoparticles shown smaller size hence better performance in terms of solubility and dissolution rate as compared to NGP and NGP-g-Am.					
30033312	7	38	theme	angle	1319:1323	arg1	determination					1325:1337	Contact angle determination	1311:1337	Contact angle determination	1311:1337	Contact angle determination easily predict better hydrophilicity of CMNGP than NGP followed by NGP-g-Am.					
30033312	2	39	theme	carboxymethylated	466:482	arg1	CMNGP					459:463	CMNGP	459:463	CMNGP (carboxymethylated neem gum)	459:492	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	39	theme	carboxymethylated	466:482	arg1	gum					489:491	carboxymethylated neem gum	466:491	carboxymethylated neem gum	466:491	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	10	40	theme	order	1833:1837	arg1	kinetics					1839:1846	1° order kinetics	1830:1846	1° order kinetics of drug	1830:1854	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	8	41	theme	amorphous	1443:1451	arg1	nature					1453:1458	the amorphous nature	1439:1458	the amorphous nature of CMNGP than NGP and NGP-g-Am	1439:1489	DSC thermogram predict the amorphous nature of CMNGP than NGP and NGP-g-Am.					
30033312	0	42	theme	ultrasound	52:61	arg1	acrylamide					120:129	acrylamide	120:129	acrylamide	120:129	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	0	42	theme	ultrasound	52:61	arg1	method					92:97	ultrasound assisted solvent-antisolvent method	52:97	ultrasound assisted solvent-antisolvent method	52:97	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	6	43	theme	same	1144:1147	arg1	concentration					1149:1161	the same concentration	1140:1161	the same concentration of drug and stabilizer	1140:1184	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	10	44	theme	Prepared	1560:1567	arg1	nanosuspension					1569:1582	Prepared nanosuspension	1560:1582	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1)	1560:1739	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	2	45	dep	NGP-g-Am	405:412	arg1	copolymer					432:440	acrylamide graft copolymer	415:440	acrylamide graft copolymer of neem gum	415:452	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	3	46	theme	independent	669:679	arg1	variables					681:689	two independent variables	665:689	two independent variables (concentration of drug and polymer)	665:725	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	2	47	theme	semisynthetic	379:391	arg1	derivatives					393:403	their semisynthetic derivatives	373:403	their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum)	373:492	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	47	theme	semisynthetic	379:391	arg1	CMNGP					459:463	CMNGP	459:463	CMNGP (carboxymethylated neem gum)	459:492	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	47	theme	semisynthetic	379:391	arg1	NGP-g-Am					405:412	NGP-g-Am	405:412	NGP-g-Am (acrylamide graft copolymer of neem gum)	405:453	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	5	48	theme	solubility	1038:1047	arg1	terms					1029:1033	terms	1029:1033	terms of solubility and dissolution rate	1029:1068	CMNGP stabilized composite nanoparticles shown smaller size hence better performance in terms of solubility and dissolution rate as compared to NGP and NGP-g-Am.					
30033312	6	49	theme	seizer	1120:1125	arg1	analysis					1127:1134	zeta seizer analysis	1115:1134	zeta seizer analysis for the same concentration of drug and stabilizer	1115:1184	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	1	50	theme	etoricoxib	240:249	arg1	nanoparticles					251:263	etoricoxib nanoparticles	240:263	etoricoxib nanoparticles	240:263	In present research, etoricoxib nanoparticles were prepared using newly developed ultrasonication assisted solvent-antisolvent method.					
30033312	4	51	theme	composite	875:883	arg1	nanoparticles					885:897	polymer stabilized molecular composite nanoparticles	846:897	polymer stabilized molecular composite nanoparticles	846:897	When compared with pure drug nanocrystals, polymer stabilized molecular composite nanoparticles were of smaller size and spherical shape.					
30033312	6	52	theme	dissolution	1293:1303	arg1	rate					1305:1308	dissolution rate	1293:1308	dissolution rate	1293:1308	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	3	53	dep	variables	681:689	arg1	concentration					692:704	concentration	692:704	concentration of drug and polymer	692:724	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	0	54	theme	drug	205:208	arg1	release					210:216	drug release	205:216	drug release	205:216	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	3	55	theme	surface	577:583	arg1	methodology					585:595	response surface methodology	568:595	response surface methodology based on 32 factorial design	568:624	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	4	56	theme	stabilized	854:863	arg1	nanoparticles					885:897	polymer stabilized molecular composite nanoparticles	846:897	polymer stabilized molecular composite nanoparticles	846:897	When compared with pure drug nanocrystals, polymer stabilized molecular composite nanoparticles were of smaller size and spherical shape.					
30033312	5	57	dep	solubility	1038:1047	arg1	rate					1065:1068	rate	1065:1068	rate	1065:1068	CMNGP stabilized composite nanoparticles shown smaller size hence better performance in terms of solubility and dissolution rate as compared to NGP and NGP-g-Am.					
30033312	10	58	theme	formulations	1686:1697	arg1	C1					1737:1738	C1	1737:1738	C1	1737:1738	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	10	58	theme	formulations	1686:1697	arg1	A8					1722:1723	A8	1722:1723	A8	1722:1723	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	10	58	theme	formulations	1686:1697	arg1	%					1671:1671	t90%	1668:1671	t90% of optimized formulations of NGP (N9)	1668:1709	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	10	58	theme	formulations	1686:1697	arg1	CMNGP					1730:1734	CMNGP	1730:1734	CMNGP (C1)	1730:1739	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	10	58	theme	formulations	1686:1697	arg1	formulations					1686:1697	optimized formulations	1676:1697	optimized formulations of NGP (N9)	1676:1709	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	10	58	theme	formulations	1686:1697	arg1	NGP-g-Am					1712:1719	NGP-g-Am	1712:1719	NGP-g-Am (A8)	1712:1724	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	3	59	theme	entrapment	741:750	arg1	efficiency					752:761	entrapment efficiency	741:761	entrapment efficiency	741:761	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	11	60	theme	different	1946:1954	arg1	nanoparticles					1975:1987	three different polymer stabilized nanoparticles	1940:1987	three different polymer stabilized nanoparticles	1940:1987	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
30033312	6	61	theme	NGP-g-Am > NGP > CMNGP	1236:1257	arg1	range					1227:1231	the range	1223:1231	the range of NGP-g-Am > NGP > CMNGP	1223:1257	As shown by zeta seizer analysis for the same concentration of drug and stabilizer, size of nanoparticles were found in the range of NGP-g-Am > NGP > CMNGP and reverse case was observed for dissolution rate.					
30033312	0	62	theme	carboxymethylated	152:168	arg1	gum					175:177	carboxymethylated neem gum	152:177	carboxymethylated neem gum	152:177	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	4	63	theme	smaller	907:913	arg1	size					915:918	smaller size	907:918	smaller size	907:918	When compared with pure drug nanocrystals, polymer stabilized molecular composite nanoparticles were of smaller size and spherical shape.					
30033312	10	64	theme	1°	1830:1831	arg1	kinetics					1839:1846	1° order kinetics	1830:1846	1° order kinetics of drug	1830:1854	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	12	65	theme	solvent	2087:2093	arg1	presence					2075:2082	presence	2075:2082	presence of solvent	2075:2093	Particle size of nanoparticles was studied in presence of solvent and findings showed no change in size after 45 days.					
30033312	12	66	theme	nanoparticles	2046:2058	arg1	size					2038:2041	Particle size	2029:2041	Particle size of nanoparticles	2029:2058	Particle size of nanoparticles was studied in presence of solvent and findings showed no change in size after 45 days.					
30033312	11	67	from	nanoparticles	1975:1987	arg1	pattern					1919:1925	release pattern	1911:1925	release pattern of drug from three different polymer stabilized nanoparticles	1911:1987	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
30033312	11	67	from	nanoparticles	1975:1987	arg1	drug					1930:1933	drug	1930:1933	drug from three different polymer stabilized nanoparticles	1930:1987	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
30033312	11	68	theme	polymer	1956:1962	arg1	nanoparticles					1975:1987	three different polymer stabilized nanoparticles	1940:1987	three different polymer stabilized nanoparticles	1940:1987	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
30033312	3	69	used	utilized	630:637	arg2	methodology					585:595	response surface methodology	568:595	response surface methodology based on 32 factorial design	568:624	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	0	70	theme	neem	110:113	arg1	gum					115:117	neem gum	110:117	neem gum	110:117	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	5	71	theme	better	1007:1012	arg1	performance					1014:1024	smaller size hence better performance	988:1024	smaller size hence better performance	988:1024	CMNGP stabilized composite nanoparticles shown smaller size hence better performance in terms of solubility and dissolution rate as compared to NGP and NGP-g-Am.					
30033312	5	72	theme	composite	958:966	arg1	nanoparticles					968:980	composite nanoparticles	958:980	composite nanoparticles shown smaller size hence better performance in terms of solubility and dissolution rate	958:1068	CMNGP stabilized composite nanoparticles shown smaller size hence better performance in terms of solubility and dissolution rate as compared to NGP and NGP-g-Am.					
30033312	0	73	theme	nanoparticles	26:38	arg1	facilitation					10:21	Stability facilitation	0:21	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide	0:129	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	0	74	dep	method	92:97	arg1	Effect					100:105	Effect	100:105	Effect of neem gum	100:117	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	2	75	theme	gum	450:452	arg1	copolymer					432:440	acrylamide graft copolymer	415:440	acrylamide graft copolymer of neem gum	415:452	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	10	76	theme	drug	1851:1854	arg1	order					1811:1815	1° order	1808:1815	1° order	1808:1815	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	10	76	theme	drug	1851:1854	arg1	kinetics					1839:1846	1° order kinetics	1830:1846	1° order kinetics of drug	1830:1854	Prepared nanosuspension show no aggregation and Ostwald repining after 45 days when ultrasonicated for 5 s. t90% of optimized formulations of NGP (N9), NGP-g-Am (A8) and CMNGP (C1) was found to be 249.46 min, 296.63 min and 223.39 min and followed 1° order, Higuchi and 1° order kinetics of drug, respectively.					
30033312	11	77	theme	drug	1930:1933	arg1	pattern					1919:1925	release pattern	1911:1925	release pattern of drug from three different polymer stabilized nanoparticles	1911:1987	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
30033312	1	78	theme	ultrasonication	301:315	arg1	method					346:351	newly developed ultrasonication assisted solvent-antisolvent method	285:351	newly developed ultrasonication assisted solvent-antisolvent method	285:351	In present research, etoricoxib nanoparticles were prepared using newly developed ultrasonication assisted solvent-antisolvent method.					
30033312	7	79	theme	Contact	1311:1317	arg1	determination					1325:1337	Contact angle determination	1311:1337	Contact angle determination	1311:1337	Contact angle determination easily predict better hydrophilicity of CMNGP than NGP followed by NGP-g-Am.					
30033312	2	80	theme	neem	484:487	arg1	CMNGP					459:463	CMNGP	459:463	CMNGP (carboxymethylated neem gum)	459:492	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	80	theme	neem	484:487	arg1	gum					489:491	carboxymethylated neem gum	466:491	carboxymethylated neem gum	466:491	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	81	theme	graft	426:430	arg1	copolymer					432:440	acrylamide graft copolymer	415:440	acrylamide graft copolymer of neem gum	415:452	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	0	82	theme	assisted	63:70	arg1	acrylamide					120:129	acrylamide	120:129	acrylamide	120:129	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	0	82	theme	assisted	63:70	arg1	method					92:97	ultrasound assisted solvent-antisolvent method	52:97	ultrasound assisted solvent-antisolvent method	52:97	Stability facilitation of nanoparticles prepared by ultrasound assisted solvent-antisolvent method: Effect of neem gum, acrylamide grafted neem gum and carboxymethylated neem gum over size, morphology and drug release.					
30033312	1	83	theme	solvent-antisolvent	326:344	arg1	method					346:351	newly developed ultrasonication assisted solvent-antisolvent method	285:351	newly developed ultrasonication assisted solvent-antisolvent method	285:351	In present research, etoricoxib nanoparticles were prepared using newly developed ultrasonication assisted solvent-antisolvent method.					
30033312	3	84	theme	variables	681:689	arg1	effect					655:660	the effect	651:660	the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables)	651:800	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	4	85	theme	drug	827:830	arg1	nanocrystals					832:843	pure drug nanocrystals	822:843	pure drug nanocrystals	822:843	When compared with pure drug nanocrystals, polymer stabilized molecular composite nanoparticles were of smaller size and spherical shape.					
30033312	3	86	theme	factorial	609:617	arg1	design					619:624	32 factorial design	606:624	32 factorial design	606:624	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	2	87	used	used	499:502	arg2	derivatives					393:403	their semisynthetic derivatives	373:403	their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum)	373:492	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	87	used	used	499:502	arg2	CMNGP					459:463	CMNGP	459:463	CMNGP (carboxymethylated neem gum)	459:492	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	87	used	used	499:502	arg2	NGP					364:366	NGP	364:366	NGP	364:366	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	87	used	used	499:502	arg2	NGP-g-Am					405:412	NGP-g-Am	405:412	NGP-g-Am (acrylamide graft copolymer of neem gum)	405:453	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	87	used	used	499:502	arg2	gum					359:361	Neem gum	354:361	Neem gum (NGP)	354:367	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	88	dep	derivatives	393:403	arg1	derivatives					393:403	their semisynthetic derivatives	373:403	their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum)	373:492	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	88	dep	derivatives	393:403	arg1	CMNGP					459:463	CMNGP	459:463	CMNGP (carboxymethylated neem gum)	459:492	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	88	dep	derivatives	393:403	arg1	NGP-g-Am					405:412	NGP-g-Am	405:412	NGP-g-Am (acrylamide graft copolymer of neem gum)	405:453	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	88	dep	derivatives	393:403	arg1	gum					489:491	carboxymethylated neem gum	466:491	carboxymethylated neem gum	466:491	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	89	theme	Neem	354:357	arg1	NGP					364:366	NGP	364:366	NGP	364:366	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	2	89	theme	Neem	354:357	arg1	gum					359:361	Neem gum	354:361	Neem gum (NGP)	354:367	Neem gum (NGP) and their semisynthetic derivatives NGP-g-Am (acrylamide graft copolymer of neem gum) and CMNGP (carboxymethylated neem gum) were used to provide stability to drug nanoprecipitates.					
30033312	1	90	theme	present	222:228	arg1	research					230:237	present research	222:237	present research	222:237	In present research, etoricoxib nanoparticles were prepared using newly developed ultrasonication assisted solvent-antisolvent method.					
30033312	12	91	theme	Particle	2029:2036	arg1	size					2038:2041	Particle size	2029:2041	Particle size of nanoparticles	2029:2058	Particle size of nanoparticles was studied in presence of solvent and findings showed no change in size after 45 days.					
30033312	3	92	theme	drug	709:712	arg1	concentration					692:704	concentration	692:704	concentration of drug and polymer	692:724	In this research response surface methodology based on 32 factorial design was utilized to evaluate the effect of two independent variables (concentration of drug and polymer) over size and entrapment efficiency of nanoparticles (dependent variables).					
30033312	8	93	theme	CMNGP	1463:1467	arg1	nature					1453:1458	the amorphous nature	1439:1458	the amorphous nature of CMNGP than NGP and NGP-g-Am	1439:1489	DSC thermogram predict the amorphous nature of CMNGP than NGP and NGP-g-Am.					
30033312	11	94	theme	Similarity	1871:1880	arg1	analysis					1889:1896	Similarity factor analysis	1871:1896	Similarity factor analysis	1871:1896	Similarity factor analysis predict that release pattern of drug from three different polymer stabilized nanoparticles are significantly differ to each other.					
29055059	3	0	theme	phosphorylation	695:709	arg1	identification					651:664	the identification	647:664	the identification of protein glycosylation and phosphorylation	647:709	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	7	1	theme	positive	1176:1183	arg1	mode					1189:1192	the positive ion mode	1172:1192	the positive ion mode	1172:1192	The composition of the glycan modification was resolved in the positive ion mode, while the level of phosphorylation was investigated in the negative ion mode.					
29055059	11	2	theme	existing	1905:1912	arg1	toolkit					1914:1920	the existing toolkit	1901:1920	the existing toolkit for PTM discovery	1901:1938	CONCLUSIONS We contend that bbCID is a valuable addition to the existing toolkit for PTM discovery.					
29055059	3	3	dep	protein	669:675	arg1	glycosylation					677:689	glycosylation	677:689	glycosylation	677:689	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	10	4	theme	O-linked	1795:1802	arg1	glycopeptides					1804:1816	O-linked glycopeptides	1795:1816	O-linked glycopeptides	1795:1816	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
29055059	13	5	theme	samples	2219:2225	arg1	characterisation					2165:2180	the characterisation	2161:2180	the characterisation of individual proteins or biological samples of low complexity	2161:2243	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	5	6	theme	low	949:951	arg1	energy					972:977	low and high collision energy	949:977	low and high collision energy	949:977	In bbCID, precursor and product ion spectra were obtained by alternating low and high collision energy.					
29055059	12	7	theme	proteomics	2003:2012	arg1	methods					2014:2020	targeted proteomics methods	1994:2020	targeted proteomics methods	1994:2020	Moreover, this technique could be employed to direct targeted proteomics methods, particularly where there is no a priori information on glycosylation or phosphorylation status.					
29055059	7	8	theme	glycan	1136:1141	arg1	modification					1143:1154	the glycan modification	1132:1154	the glycan modification	1132:1154	The composition of the glycan modification was resolved in the positive ion mode, while the level of phosphorylation was investigated in the negative ion mode.					
29055059	13	9	theme	therapeutic	2312:2322	arg1	protein					2324:2330	a therapeutic protein	2310:2330	a therapeutic protein	2310:2330	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	10	10	theme	positive	1822:1829	arg1	mode					1835:1838	positive ion mode	1822:1838	positive ion mode	1822:1838	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
29055059	0	11	theme	phosphorylation	125:139	arg1	analysis					141:148	phosphorylation analysis	125:148	phosphorylation analysis	125:148	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.					
29055059	3	12	theme	collision-induced	575:591	arg1	method					621:626	a DIA method	615:626	a DIA method	615:626	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	3	12	theme	collision-induced	575:591	arg1	bbCID					607:611	bbCID	607:611	bbCID	607:611	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	3	12	theme	collision-induced	575:591	arg1	dissociation					593:604	broadband collision-induced dissociation	565:604	broadband collision-induced dissociation (bbCID)	565:612	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	9	13	theme	novel	1510:1514	arg1	use					1516:1518	The novel use	1506:1518	The novel use of bbCID in negative ion mode	1506:1548	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	8	14	theme	associated	1479:1488	arg1	ions					1500:1503	specific diagnostic and associated precursor ions	1455:1503	specific diagnostic and associated precursor ions	1455:1503	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	6	15	theme	Precursor	980:988	arg1	ions					990:993	Precursor ions	980:993	Precursor ions	980:993	Precursor ions were assigned manually based on the detection of diagnostic ions specific to either glycosylation or phosphorylation.					
29055059	8	16	theme	ions	1500:1503	arg1	detection					1442:1450	the detection	1438:1450	the detection of specific diagnostic and associated precursor ions	1438:1503	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	5	17	theme	collision	962:970	arg1	energy					972:977	low and high collision energy	949:977	low and high collision energy	949:977	In bbCID, precursor and product ion spectra were obtained by alternating low and high collision energy.					
29055059	5	18	theme	high	957:960	arg1	energy					972:977	low and high collision energy	949:977	low and high collision energy	949:977	In bbCID, precursor and product ion spectra were obtained by alternating low and high collision energy.					
29055059	8	19	theme	bbCID	1342:1346	arg1	approach					1348:1355	a bbCID approach	1340:1355	a bbCID approach	1340:1355	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	13	20	theme	biological	2208:2217	arg1	samples					2219:2225	biological samples	2208:2225	biological samples	2208:2225	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	1	21	theme	data-dependent	342:355	arg1	DDA					370:372	DDA	370:372	DDA	370:372	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	1	21	theme	data-dependent	342:355	arg1	acquisition					357:367	traditional data-dependent acquisition	330:367	traditional data-dependent acquisition (DDA)	330:373	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	6	22	theme	diagnostic	1044:1053	arg1	ions					1055:1058	diagnostic ions	1044:1058	diagnostic ions specific to either glycosylation or phosphorylation	1044:1110	Precursor ions were assigned manually based on the detection of diagnostic ions specific to either glycosylation or phosphorylation.					
29055059	2	23	theme	product	504:510	arg1	ions					512:515	all detectable precursor and product ions	475:515	ions	512:515	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	12	24	theme	phosphorylation	2095:2109	arg1	status					2111:2116	phosphorylation status	2095:2116	phosphorylation status	2095:2116	Moreover, this technique could be employed to direct targeted proteomics methods, particularly where there is no a priori information on glycosylation or phosphorylation status.					
29055059	0	25	theme	spectrometry	73:84	arg1	approach					86:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.					
29055059	11	26	dep	CONCLUSIONS	1841:1851	arg1	contend					1856:1862	contend	1856:1862	contend that bbCID is a valuable addition to the existing toolkit for PTM discovery	1856:1938	CONCLUSIONS We contend that bbCID is a valuable addition to the existing toolkit for PTM discovery.					
29055059	1	27	theme	modified	194:201	arg1	peptides					203:210	post-translationally modified peptides	173:210	post-translationally modified peptides	173:210	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	2	28	theme	intensity	543:551	arg1	independent					518:528	independent	518:528	independent	518:528	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	4	29	theme	standard	799:806	arg1	proteins					808:815	standard proteins	799:815	standard proteins using a high-resolution Bruker maXis 3G mass spectrometer	799:873	METHODS bbCID was applied to identify glycopeptides and phosphopeptides generated from standard proteins using a high-resolution Bruker maXis 3G mass spectrometer.					
29055059	9	30	theme	ion	1541:1543	arg1	mode					1545:1548	negative ion mode	1532:1548	negative ion mode	1532:1548	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	13	31	gly	glycosylation	2286:2298	arg1	protein					2324:2330	a therapeutic protein	2310:2330	a therapeutic protein	2310:2330	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	2	32	theme	data-independent	404:419	arg1	DIA					434:436	DIA	434:436	DIA	434:436	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	2	32	theme	data-independent	404:419	arg1	approach					394:401	An alternative MS approach	376:401	An alternative MS approach	376:401	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	2	32	theme	data-independent	404:419	arg1	acquisition					421:431	data-independent acquisition	404:431	data-independent acquisition (DIA)	404:437	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	6	33	theme	specific	1060:1067	arg1	ions					1055:1058	diagnostic ions	1044:1058	diagnostic ions specific to either glycosylation or phosphorylation	1044:1110	Precursor ions were assigned manually based on the detection of diagnostic ions specific to either glycosylation or phosphorylation.					
29055059	1	34	theme	RATIONALE	151:159	arg1	Analysis					161:168	RATIONALE Analysis	151:168	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS)	151:236	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	8	35	theme	glycopeptides	1395:1407	arg1	identification					1377:1390	the identification	1373:1390	the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions	1373:1503	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	11	36	theme	PTM	1926:1928	arg1	discovery					1930:1938	PTM discovery	1926:1938	PTM discovery	1926:1938	CONCLUSIONS We contend that bbCID is a valuable addition to the existing toolkit for PTM discovery.					
29055059	7	37	theme	phosphorylation	1214:1228	arg1	level					1205:1209	the level	1201:1209	the level of phosphorylation	1201:1228	The composition of the glycan modification was resolved in the positive ion mode, while the level of phosphorylation was investigated in the negative ion mode.					
29055059	9	38	theme	peptides	1615:1622	arg1	discrimination					1562:1575	the discrimination	1558:1575	the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions	1558:1674	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	8	39	theme	phosphopeptides	1413:1427	arg1	identification					1377:1390	the identification	1373:1390	the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions	1373:1503	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	10	40	theme	approach	1726:1733	arg1	ability					1710:1716	the ability	1706:1716	the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides	1706:1816	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
29055059	1	41	theme	peptides	300:307	arg1	sampling					284:291	incomplete sampling	273:291	incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA)	273:373	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	8	42	dep	RESULTS	1273:1279	arg1	demonstrate					1293:1303	demonstrate	1293:1303	demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions	1293:1503	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	2	43	theme	precursor	490:498	arg1	recording					462:470	comprehensive recording	448:470	comprehensive recording of all detectable precursor and product ions	448:515	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	4	44	theme	mass	857:860	arg1	spectrometer					862:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	METHODS bbCID was applied to identify glycopeptides and phosphopeptides generated from standard proteins using a high-resolution Bruker maXis 3G mass spectrometer.					
29055059	0	45	theme	collision-induced	29:45	arg1	approach					86:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.					
29055059	9	46	from	use	1516:1518	arg1	mode					1545:1548	negative ion mode	1532:1548	negative ion mode	1532:1548	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	9	47	theme	phosphate	1650:1658	arg1	ions					1671:1674	phosphate diagnostic ions	1650:1674	phosphate diagnostic ions	1650:1674	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	8	48	theme	specific	1455:1462	arg1	ions					1500:1503	specific diagnostic and associated precursor ions	1455:1503	specific diagnostic and associated precursor ions	1455:1503	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	9	49	theme	ions	1671:1674	arg1	detection					1637:1645	the detection	1633:1645	the detection of phosphate diagnostic ions	1633:1674	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	3	50	theme	protein	669:675	arg1	identification					651:664	the identification	647:664	the identification of protein glycosylation and phosphorylation	647:709	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	4	51	theme	maXis	848:852	arg1	spectrometer					862:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	METHODS bbCID was applied to identify glycopeptides and phosphopeptides generated from standard proteins using a high-resolution Bruker maXis 3G mass spectrometer.					
29055059	2	52	theme	alternative	379:389	arg1	approach					394:401	An alternative MS approach	376:401	An alternative MS approach	376:401	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	2	52	theme	alternative	379:389	arg1	acquisition					421:431	data-independent acquisition	404:431	data-independent acquisition (DIA)	404:437	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	13	53	theme	complexity	2234:2243	arg1	proteins					2196:2203	individual proteins	2185:2203	individual proteins	2185:2203	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	13	53	theme	complexity	2234:2243	arg1	samples					2219:2225	biological samples	2208:2225	biological samples	2208:2225	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	0	54	theme	bbCID	61:65	arg1	approach					86:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.					
29055059	12	55	from	information	2063:2073	arg1	glycosylation					2078:2090	glycosylation	2078:2090	glycosylation	2078:2090	Moreover, this technique could be employed to direct targeted proteomics methods, particularly where there is no a priori information on glycosylation or phosphorylation status.					
29055059	12	55	from	information	2063:2073	arg1	status					2111:2116	phosphorylation status	2095:2116	phosphorylation status	2095:2116	Moreover, this technique could be employed to direct targeted proteomics methods, particularly where there is no a priori information on glycosylation or phosphorylation status.					
29055059	8	56	gly	glycopeptides	1395:1407	arg2	glycopeptides					1395:1407	glycopeptides	1395:1407	glycopeptides	1395:1407	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	7	57	theme	negative	1254:1261	arg1	mode					1267:1270	the negative ion mode	1250:1270	the negative ion mode	1250:1270	The composition of the glycan modification was resolved in the positive ion mode, while the level of phosphorylation was investigated in the negative ion mode.					
29055059	10	58	theme	glycan	1766:1771	arg1	composition					1773:1783	glycan composition	1766:1783	glycan composition	1766:1783	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
29055059	13	59	theme	proteins	2196:2203	arg1	characterisation					2165:2180	the characterisation	2161:2180	the characterisation of individual proteins or biological samples of low complexity	2161:2243	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	12	60	theme	priori	2056:2061	arg1	information					2063:2073	no a priori information	2051:2073	no a priori information on glycosylation or phosphorylation status	2051:2116	Moreover, this technique could be employed to direct targeted proteomics methods, particularly where there is no a priori information on glycosylation or phosphorylation status.					
29055059	1	61	theme	incomplete	273:282	arg1	sampling					284:291	incomplete sampling	273:291	incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA)	273:373	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	3	62	theme	dissociation	593:604	arg1	use					558:560	The use	554:560	The use of broadband collision-induced dissociation (bbCID), a DIA method,	554:627	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	10	63	from	identification	1748:1761	arg1	N-					1788:1789	N-	1788:1789	N-	1788:1789	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
29055059	10	63	from	identification	1748:1761	arg1	glycopeptides					1804:1816	O-linked glycopeptides	1795:1816	O-linked glycopeptides	1795:1816	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
29055059	7	64	theme	modification	1143:1154	arg1	composition					1117:1127	The composition	1113:1127	The composition of the glycan modification	1113:1154	The composition of the glycan modification was resolved in the positive ion mode, while the level of phosphorylation was investigated in the negative ion mode.					
29055059	1	65	theme	mass	215:218	arg1	MS					234:235	MS	234:235	MS	234:235	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	1	65	theme	mass	215:218	arg1	spectrometry					220:231	mass spectrometry	215:231	mass spectrometry (MS)	215:236	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	1	66	theme	peptides	203:210	arg1	Analysis					161:168	RATIONALE Analysis	151:168	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS)	151:236	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	7	67	theme	ion	1263:1265	arg1	mode					1267:1270	the negative ion mode	1250:1270	the negative ion mode	1250:1270	The composition of the glycan modification was resolved in the positive ion mode, while the level of phosphorylation was investigated in the negative ion mode.					
29055059	10	68	theme	ion	1831:1833	arg1	mode					1835:1838	positive ion mode	1822:1838	positive ion mode	1822:1838	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
29055059	5	69	theme	ion	908:910	arg1	spectra					912:918	ion spectra	908:918	ion spectra	908:918	In bbCID, precursor and product ion spectra were obtained by alternating low and high collision energy.					
29055059	12	70	theme	targeted	1994:2001	arg1	methods					2014:2020	targeted proteomics methods	1994:2020	targeted proteomics methods	1994:2020	Moreover, this technique could be employed to direct targeted proteomics methods, particularly where there is no a priori information on glycosylation or phosphorylation status.					
29055059	3	71	theme	broadband	565:573	arg1	method					621:626	a DIA method	615:626	a DIA method	615:626	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	3	71	theme	broadband	565:573	arg1	bbCID					607:611	bbCID	607:611	bbCID	607:611	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	3	71	theme	broadband	565:573	arg1	dissociation					593:604	broadband collision-induced dissociation	565:604	broadband collision-induced dissociation (bbCID)	565:612	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	4	72	theme	Bruker	841:846	arg1	spectrometer					862:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	METHODS bbCID was applied to identify glycopeptides and phosphopeptides generated from standard proteins using a high-resolution Bruker maXis 3G mass spectrometer.					
29055059	8	73	theme	precursor	1490:1498	arg1	ions					1500:1503	specific diagnostic and associated precursor ions	1455:1503	specific diagnostic and associated precursor ions	1455:1503	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	8	74	theme	approach	1348:1355	arg1	use					1333:1335	the use	1329:1335	the use of a bbCID approach	1329:1355	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	8	74	theme	approach	1348:1355	arg1	suitable					1360:1367	suitable	1360:1367	suitable	1360:1367	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	10	75	link	O-linked	1795:1802	arg1	glycopeptides					1804:1816	O-linked glycopeptides	1795:1816	O-linked glycopeptides	1795:1816	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
29055059	8	76	theme	first	1313:1317	arg1	time					1319:1322	the first time	1309:1322	the first time	1309:1322	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	4	77	gly	glycopeptides	750:762	arg2	glycopeptides					750:762	glycopeptides	750:762	glycopeptides	750:762	METHODS bbCID was applied to identify glycopeptides and phosphopeptides generated from standard proteins using a high-resolution Bruker maXis 3G mass spectrometer.					
29055059	13	78	theme	individual	2185:2194	arg1	proteins					2196:2203	individual proteins	2185:2203	individual proteins	2185:2203	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	9	79	theme	bbCID	1523:1527	arg1	use					1516:1518	The novel use	1506:1518	The novel use of bbCID in negative ion mode	1506:1548	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	6	80	dep	assigned	1000:1007	arg1	based					1018:1022	based	1018:1022	were assigned manually based on the detection of diagnostic ions specific to either glycosylation or phosphorylation	995:1110	Precursor ions were assigned manually based on the detection of diagnostic ions specific to either glycosylation or phosphorylation.					
29055059	4	81	theme	high-resolution	825:839	arg1	spectrometer					862:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	METHODS bbCID was applied to identify glycopeptides and phosphopeptides generated from standard proteins using a high-resolution Bruker maXis 3G mass spectrometer.					
29055059	9	82	theme	negative	1532:1539	arg1	mode					1545:1548	negative ion mode	1532:1548	negative ion mode	1532:1548	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	0	83	theme	mass	68:71	arg1	approach					86:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.					
29055059	3	84	theme	DIA	617:619	arg1	method					621:626	a DIA method	615:626	a DIA method	615:626	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	3	84	theme	DIA	617:619	arg1	dissociation					593:604	broadband collision-induced dissociation	565:604	broadband collision-induced dissociation (bbCID)	565:612	The use of broadband collision-induced dissociation (bbCID), a DIA method, was evaluated for the identification of protein glycosylation and phosphorylation.					
29055059	13	85	theme	status	2300:2305	arg1	analysis					2270:2277	the analysis	2266:2277	the analysis of the glycosylation status of a therapeutic protein	2266:2330	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	6	86	theme	ions	1055:1058	arg1	detection					1031:1039	the detection	1027:1039	the detection of diagnostic ions specific to either glycosylation or phosphorylation	1027:1110	Precursor ions were assigned manually based on the detection of diagnostic ions specific to either glycosylation or phosphorylation.					
29055059	2	87	theme	ions	512:515	arg1	recording					462:470	comprehensive recording	448:470	comprehensive recording of all detectable precursor and product ions	448:515	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	0	88	theme	approach	86:93	arg1	Application					0:10	Application	0:10	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.	0:149	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.					
29055059	4	89	theme	METHODS	712:718	arg1	bbCID					720:724	METHODS bbCID	712:724	METHODS bbCID	712:724	METHODS bbCID was applied to identify glycopeptides and phosphopeptides generated from standard proteins using a high-resolution Bruker maXis 3G mass spectrometer.					
29055059	0	90	theme	protein	99:105	arg1	glycosylation					107:119	protein glycosylation	99:119	protein glycosylation	99:119	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.					
29055059	2	91	theme	precursor	533:541	arg1	intensity					543:551	precursor intensity	533:551	precursor intensity	533:551	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	9	92	theme	phosphorylated	1600:1613	arg1	peptides					1615:1622	singly and multiply phosphorylated peptides	1580:1622	singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions	1580:1674	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	2	93	theme	comprehensive	448:460	arg1	recording					462:470	comprehensive recording	448:470	comprehensive recording of all detectable precursor and product ions	448:515	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	2	94	theme	MS	391:392	arg1	approach					394:401	An alternative MS approach	376:401	An alternative MS approach	376:401	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	2	94	theme	MS	391:392	arg1	acquisition					421:431	data-independent acquisition	404:431	data-independent acquisition (DIA)	404:437	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	0	95	theme	broadband	19:27	arg1	approach					86:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.					
29055059	0	96	theme	dissociation	47:58	arg1	approach					86:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	the broadband collision-induced dissociation (bbCID) mass spectrometry approach	15:93	Application of the broadband collision-induced dissociation (bbCID) mass spectrometry approach for protein glycosylation and phosphorylation analysis.					
29055059	13	97	theme	glycosylation	2286:2298	arg1	status					2300:2305	the glycosylation status	2282:2305	the glycosylation status of a therapeutic protein	2282:2330	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	2	98	theme	detectable	479:488	arg1	precursor					490:498	all detectable precursor and product ions	475:515	precursor	490:498	An alternative MS approach, data-independent acquisition (DIA), enables comprehensive recording of all detectable precursor and product ions, independent of precursor intensity.					
29055059	4	99	theme	3G	854:855	arg1	spectrometer					862:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	a high-resolution Bruker maXis 3G mass spectrometer	823:873	METHODS bbCID was applied to identify glycopeptides and phosphopeptides generated from standard proteins using a high-resolution Bruker maXis 3G mass spectrometer.					
29055059	7	100	theme	ion	1185:1187	arg1	mode					1189:1192	the positive ion mode	1172:1192	the positive ion mode	1172:1192	The composition of the glycan modification was resolved in the positive ion mode, while the level of phosphorylation was investigated in the negative ion mode.					
29055059	10	101	gly	glycopeptides	1804:1816	arg2	glycopeptides					1804:1816	O-linked glycopeptides	1795:1816	O-linked glycopeptides	1795:1816	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
29055059	1	102	theme	traditional	330:340	arg1	DDA					370:372	DDA	370:372	DDA	370:372	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	1	102	theme	traditional	330:340	arg1	acquisition					357:367	traditional data-dependent acquisition	330:367	traditional data-dependent acquisition (DDA)	330:373	RATIONALE Analysis of post-translationally modified peptides by mass spectrometry (MS) remains incomplete, in part due to incomplete sampling of all peptides which is inherent to traditional data-dependent acquisition (DDA).					
29055059	9	103	theme	diagnostic	1660:1669	arg1	ions					1671:1674	phosphate diagnostic ions	1650:1674	phosphate diagnostic ions	1650:1674	The novel use of bbCID in negative ion mode allowed the discrimination of singly and multiply phosphorylated peptides based on the detection of phosphate diagnostic ions.					
29055059	5	104	dep	precursor	886:894	arg1	spectra					912:918	ion spectra	908:918	ion spectra	908:918	In bbCID, precursor and product ion spectra were obtained by alternating low and high collision energy.					
29055059	11	105	theme	valuable	1880:1887	arg1	addition					1889:1896	a valuable addition	1878:1896	a valuable addition to the existing toolkit for PTM discovery	1878:1938	CONCLUSIONS We contend that bbCID is a valuable addition to the existing toolkit for PTM discovery.					
29055059	11	105	theme	valuable	1880:1887	arg1	bbCID					1869:1873	bbCID	1869:1873	bbCID	1869:1873	CONCLUSIONS We contend that bbCID is a valuable addition to the existing toolkit for PTM discovery.					
29055059	13	106	theme	low	2230:2232	arg1	complexity					2234:2243	low complexity	2230:2243	low complexity	2230:2243	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	8	107	theme	diagnostic	1464:1473	arg1	ions					1500:1503	specific diagnostic and associated precursor ions	1455:1503	specific diagnostic and associated precursor ions	1455:1503	RESULTS The results demonstrate for the first time that the use of a bbCID approach is suitable for the identification of glycopeptides and phosphopeptides based on the detection of specific diagnostic and associated precursor ions.					
29055059	13	108	theme	protein	2324:2330	arg1	status					2300:2305	the glycosylation status	2282:2305	the glycosylation status of a therapeutic protein	2282:2330	This technique is immediately relevant to the characterisation of individual proteins or biological samples of low complexity, as demonstrated for the analysis of the glycosylation status of a therapeutic protein.					
29055059	10	109	theme	composition	1773:1783	arg1	identification					1748:1761	the identification	1744:1761	the identification of glycan composition in N- and O-linked glycopeptides	1744:1816	The results also demonstrate the ability of this approach to allow the identification of glycan composition in N- and O-linked glycopeptides, in positive ion mode.					
30583452	0	0	theme	moisture	70:77	arg1	stress					79:84	soil moisture stress	65:84	soil moisture stress	65:84	Soybean seed physiology, quality, and chemical composition under soil moisture stress.					
30583452	4	1	theme	oleic	619:623	arg1	acids					639:643	stearic, oleic and linolenic acids	610:643	stearic, oleic and linolenic acids	610:643	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	1	theme	oleic	619:623	arg1	oil					605:607	oil	605:607	oil	605:607	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	0	2	theme	soil	65:68	arg1	stress					79:84	soil moisture stress	65:84	soil moisture stress	65:84	Soybean seed physiology, quality, and chemical composition under soil moisture stress.					
30583452	7	3	theme	seed	1017:1020	arg1	formation					1022:1030	seed formation	1017:1030	seed formation	1017:1030	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
30583452	7	4	theme	seeds	1089:1093	arg1	value					1072:1076	the higher nutritional value	1049:1076	the higher nutritional value of soybean seeds	1049:1093	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
30583452	4	5	theme	Seed	483:486	arg1	acids					519:523	palmitic and linoleic acids	497:523	palmitic and linoleic acids	497:523	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	5	theme	Seed	483:486	arg1	protein					488:494	Seed protein	483:494	Seed protein	483:494	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	2	6	theme	soil	270:273	arg1	stress					284:289	soil moisture stress	270:289	soil moisture stress during reproductive stage	270:315	The objective of this study was to investigate the effects of soil moisture stress during reproductive stage on seed quality and composition.					
30583452	2	7	theme	moisture	275:282	arg1	stress					284:289	soil moisture stress	270:289	soil moisture stress during reproductive stage	270:315	The objective of this study was to investigate the effects of soil moisture stress during reproductive stage on seed quality and composition.					
30583452	7	8	theme	flowering	1003:1011	arg1	stages					1032:1037	flowering and seed formation stages	1003:1037	flowering and seed formation stages	1003:1037	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
30583452	6	9	theme	nutrient	852:859	arg1	accumulation					861:872	nutrient accumulation	852:872	nutrient accumulation	852:872	The changes in seed constituents could be due to changes in nutrient accumulation and partitioning in soybean seeds under water stress.					
30583452	6	10	from	accumulation	861:872	arg1	seeds					902:906	soybean seeds	894:906	soybean seeds under water stress	894:925	The changes in seed constituents could be due to changes in nutrient accumulation and partitioning in soybean seeds under water stress.					
30583452	3	11	theme	quality	441:447	arg1	traits					449:454	yield and quality traits	431:454	yield and quality traits	431:454	Plants were subjected to five levels of soil moisture stresses at flowering, and yield and quality traits were examined at maturity.					
30583452	7	12	theme	soybean	1081:1087	arg1	seeds					1089:1093	soybean seeds	1081:1093	soybean seeds	1081:1093	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
30583452	7	13	theme	moisture	987:994	arg1	requirement					958:968	the requirement	954:968	the requirement of adequate soil moisture	954:994	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
30583452	5	14	theme	seed	744:747	arg1	protein					749:755	seed protein	744:755	seed protein	744:755	The relationship between seed protein and oil was negatively correlated.					
30583452	7	15	theme	nutritional	1060:1070	arg1	value					1072:1076	the higher nutritional value	1049:1076	the higher nutritional value of soybean seeds	1049:1093	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
30583452	6	16	from	changes	796:802	arg1	constituents					812:823	seed constituents	807:823	seed constituents	807:823	The changes in seed constituents could be due to changes in nutrient accumulation and partitioning in soybean seeds under water stress.					
30583452	6	17	from	partitioning	878:889	arg1	seeds					902:906	soybean seeds	894:906	soybean seeds under water stress	894:925	The changes in seed constituents could be due to changes in nutrient accumulation and partitioning in soybean seeds under water stress.					
30583452	2	18	theme	reproductive	298:309	arg1	stage					311:315	reproductive stage	298:315	reproductive stage	298:315	The objective of this study was to investigate the effects of soil moisture stress during reproductive stage on seed quality and composition.					
30583452	3	19	theme	stresses	404:411	arg1	levels					380:385	five levels	375:385	five levels of soil moisture stresses at flowering	375:424	Plants were subjected to five levels of soil moisture stresses at flowering, and yield and quality traits were examined at maturity.					
30583452	7	20	theme	adequate	973:980	arg1	moisture					987:994	adequate soil moisture	973:994	adequate soil moisture	973:994	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
30583452	7	21	theme	soil	982:985	arg1	moisture					987:994	adequate soil moisture	973:994	adequate soil moisture	973:994	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
30583452	2	22	theme	stress	284:289	arg1	effects					259:265	the effects	255:265	the effects of soil moisture stress during reproductive stage on seed quality and composition	255:347	The objective of this study was to investigate the effects of soil moisture stress during reproductive stage on seed quality and composition.					
30583452	1	23	theme	overall	179:185	arg1	aspects					199:205	overall nutritional aspects	179:205	overall nutritional aspects	179:205	Soybean seed quality is often determined by its constituents which are important to sustain overall nutritional aspects.					
30583452	4	24	dep	deficiency	707:716	arg1	response					681:688	response	681:688	response	681:688	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	1	25	theme	seed	95:98	arg1	quality					100:106	Soybean seed quality	87:106	Soybean seed quality	87:106	Soybean seed quality is often determined by its constituents which are important to sustain overall nutritional aspects.					
30583452	1	26	theme	nutritional	187:197	arg1	aspects					199:205	overall nutritional aspects	179:205	overall nutritional aspects	179:205	Soybean seed quality is often determined by its constituents which are important to sustain overall nutritional aspects.					
30583452	3	27	theme	soil	390:393	arg1	stresses					404:411	soil moisture stresses	390:411	soil moisture stresses at flowering	390:424	Plants were subjected to five levels of soil moisture stresses at flowering, and yield and quality traits were examined at maturity.					
30583452	3	28	theme	moisture	395:402	arg1	stresses					404:411	soil moisture stresses	390:411	soil moisture stresses at flowering	390:424	Plants were subjected to five levels of soil moisture stresses at flowering, and yield and quality traits were examined at maturity.					
30583452	4	29	theme	palmitic	497:504	arg1	stachyose					546:554	stachyose	546:554	stachyose	546:554	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	29	theme	palmitic	497:504	arg1	K					563:563	K	563:563	K	563:563	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	29	theme	palmitic	497:504	arg1	acids					519:523	palmitic and linoleic acids	497:523	palmitic and linoleic acids	497:523	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	29	theme	palmitic	497:504	arg1	protein					488:494	Seed protein	483:494	Seed protein	483:494	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	29	theme	palmitic	497:504	arg1	Ca					570:571	Ca	570:571	Ca	570:571	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	29	theme	palmitic	497:504	arg1	sucrose					526:532	sucrose	526:532	sucrose	526:532	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	29	theme	palmitic	497:504	arg1	N					557:557	N	557:557	N	557:557	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	29	theme	palmitic	497:504	arg1	P					560:560	P	560:560	P	560:560	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	29	theme	palmitic	497:504	arg1	raffinose					535:543	raffinose	535:543	raffinose	535:543	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	6	30	from	changes	841:847	arg1	partitioning					878:889	partitioning	878:889	partitioning	878:889	The changes in seed constituents could be due to changes in nutrient accumulation and partitioning in soybean seeds under water stress.					
30583452	6	30	from	changes	841:847	arg1	accumulation					861:872	nutrient accumulation	852:872	nutrient accumulation	852:872	The changes in seed constituents could be due to changes in nutrient accumulation and partitioning in soybean seeds under water stress.					
30583452	3	31	theme	yield	431:435	arg1	traits					449:454	yield and quality traits	431:454	yield and quality traits	431:454	Plants were subjected to five levels of soil moisture stresses at flowering, and yield and quality traits were examined at maturity.					
30583452	0	32	theme	chemical	38:45	arg1	composition					47:57	chemical composition	38:57	chemical composition	38:57	Soybean seed physiology, quality, and chemical composition under soil moisture stress.					
30583452	4	33	theme	moisture	698:705	arg1	deficiency					707:716	soil moisture deficiency	693:716	soil moisture deficiency	693:716	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	34	theme	linolenic	629:637	arg1	acids					639:643	stearic, oleic and linolenic acids	610:643	stearic, oleic and linolenic acids	610:643	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	34	theme	linolenic	629:637	arg1	oil					605:607	oil	605:607	oil	605:607	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	35	theme	linoleic	510:517	arg1	stachyose					546:554	stachyose	546:554	stachyose	546:554	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	35	theme	linoleic	510:517	arg1	K					563:563	K	563:563	K	563:563	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	35	theme	linoleic	510:517	arg1	acids					519:523	palmitic and linoleic acids	497:523	palmitic and linoleic acids	497:523	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	35	theme	linoleic	510:517	arg1	protein					488:494	Seed protein	483:494	Seed protein	483:494	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	35	theme	linoleic	510:517	arg1	Ca					570:571	Ca	570:571	Ca	570:571	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	35	theme	linoleic	510:517	arg1	sucrose					526:532	sucrose	526:532	sucrose	526:532	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	35	theme	linoleic	510:517	arg1	N					557:557	N	557:557	N	557:557	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	35	theme	linoleic	510:517	arg1	P					560:560	P	560:560	P	560:560	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	35	theme	linoleic	510:517	arg1	raffinose					535:543	raffinose	535:543	raffinose	535:543	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	36	theme	soil	693:696	arg1	deficiency					707:716	soil moisture deficiency	693:716	soil moisture deficiency	693:716	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	2	37	theme	seed	320:323	arg1	quality					325:331	seed quality	320:331	seed quality	320:331	The objective of this study was to investigate the effects of soil moisture stress during reproductive stage on seed quality and composition.					
30583452	2	38	from	effects	259:265	arg1	composition					337:347	composition	337:347	composition	337:347	The objective of this study was to investigate the effects of soil moisture stress during reproductive stage on seed quality and composition.					
30583452	2	38	from	effects	259:265	arg1	quality					325:331	seed quality	320:331	seed quality	320:331	The objective of this study was to investigate the effects of soil moisture stress during reproductive stage on seed quality and composition.					
30583452	6	39	theme	seed	807:810	arg1	constituents					812:823	seed constituents	807:823	seed constituents	807:823	The changes in seed constituents could be due to changes in nutrient accumulation and partitioning in soybean seeds under water stress.					
30583452	1	40	theme	Soybean	87:93	arg1	quality					100:106	Soybean seed quality	87:106	Soybean seed quality	87:106	Soybean seed quality is often determined by its constituents which are important to sustain overall nutritional aspects.					
30583452	6	41	theme	water	914:918	arg1	stress					920:925	water stress	914:925	water stress	914:925	The changes in seed constituents could be due to changes in nutrient accumulation and partitioning in soybean seeds under water stress.					
30583452	4	42	theme	stearic	610:616	arg1	acids					639:643	stearic, oleic and linolenic acids	610:643	stearic, oleic and linolenic acids	610:643	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	4	42	theme	stearic	610:616	arg1	oil					605:607	oil	605:607	oil	605:607	Seed protein, palmitic and linoleic acids, sucrose, raffinose, stachyose, N, P, K, and Ca significantly decreased whereas oil, stearic, oleic and linolenic acids, Fe, Mg, Zn, Cu, and B increased in response to soil moisture deficiency.					
30583452	6	43	theme	soybean	894:900	arg1	seeds					902:906	soybean seeds	894:906	soybean seeds under water stress	894:925	The changes in seed constituents could be due to changes in nutrient accumulation and partitioning in soybean seeds under water stress.					
30583452	7	44	theme	formation	1022:1030	arg1	stages					1032:1037	flowering and seed formation stages	1003:1037	flowering and seed formation stages	1003:1037	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
30583452	3	45	from	levels	380:385	arg1	flowering					416:424	flowering	416:424	flowering	416:424	Plants were subjected to five levels of soil moisture stresses at flowering, and yield and quality traits were examined at maturity.					
30583452	2	46	theme	study	230:234	arg1	objective					212:220	The objective	208:220	The objective of this study	208:234	The objective of this study was to investigate the effects of soil moisture stress during reproductive stage on seed quality and composition.					
30583452	3	47	from	flowering	416:424	arg1	stresses					404:411	soil moisture stresses	390:411	soil moisture stresses at flowering	390:424	Plants were subjected to five levels of soil moisture stresses at flowering, and yield and quality traits were examined at maturity.					
30583452	3	47	from	flowering	416:424	arg1	levels					380:385	five levels	375:385	five levels of soil moisture stresses at flowering	375:424	Plants were subjected to five levels of soil moisture stresses at flowering, and yield and quality traits were examined at maturity.					
30583452	7	48	theme	higher	1053:1058	arg1	value					1072:1076	the higher nutritional value	1049:1076	the higher nutritional value of soybean seeds	1049:1093	This information suggests the requirement of adequate soil moisture during flowering and seed formation stages to obtain the higher nutritional value of soybean seeds.					
29809295	2	0	theme	inflamed	491:498	arg1	environment					500:510	an inflamed environment	488:510	an inflamed environment	488:510	However, the inflammatory environment of the damaged joint might compromise outcomes, and this may be impacted by the choice of cell source in terms of their ability to operate anabolically in an inflamed environment.					
29809295	5	1	theme	chondrocyte-derived	1119:1137	arg1	constructs					1139:1148	chondrocyte-derived constructs	1119:1148	chondrocyte-derived constructs	1119:1148	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	4	2	theme	catabolic	849:857	arg1	[MMPs					896:900	matrix metalloproteinases [MMPs	870:900	matrix metalloproteinases [MMPs	870:900	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	4	2	theme	catabolic	849:857	arg1	mediators					859:867	catabolic mediators	849:867	catabolic mediators (matrix metalloproteinases [MMPs]	849:901	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	6	3	theme	functional	1313:1322	arg1	cartilage					1335:1343	functional engineered cartilage	1313:1343	functional engineered cartilage	1313:1343	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	6	4	theme	MSC-derived	1472:1482	arg1	repair					1494:1499	MSC-derived cartilage repair	1472:1499	MSC-derived cartilage repair	1472:1499	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	1	5	theme	tissue	276:281	arg1	properties					283:292	native tissue properties	269:292	native tissue properties	269:292	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	4	6	theme	cell-	950:954	arg1	changes					973:979	cell- and tissue-level changes	950:979	cell- and tissue-level changes	950:979	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	7	7	theme	Orthopaedic	1509:1519	arg1	Research					1521:1528	© 2018 Orthopaedic Research	1502:1528	© 2018 Orthopaedic Research Society.	1502:1537	© 2018 Orthopaedic Research Society.					
29809295	4	8	theme	mediators	859:867	arg1	characteristics					806:820	Compositional (extracellular matrix) and functional (mechanical) characteristics	741:820	Compositional (extracellular matrix) and functional (mechanical) characteristics	741:820	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	4	8	theme	mediators	859:867	arg1	release					838:844	the release	834:844	the release of catabolic mediators (matrix metalloproteinases [MMPs]	834:901	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	4	8	theme	mediators	859:867	arg1	[NO					917:919	nitric oxide [NO	904:919	nitric oxide [NO	904:919	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	5	9	link	MSC-derived	1045:1055	arg1	constructs					1057:1066	MSC-derived constructs	1045:1066	MSC-derived constructs	1045:1066	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	1	10	with	approach	166:173	arg1	constructs					238:247	engineered constructs	227:247	engineered constructs emerging that match native tissue properties	227:292	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	4	11	theme	extracellular	756:768	arg1	matrix					770:775	extracellular matrix	756:775	extracellular matrix	756:775	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	6	12	dep	have	1287:1290	arg1	both					1282:1285	both	1282:1285	both	1282:1285	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	5	13	theme	lower	1223:1227	arg1	concentrations					1238:1251	lower cytokine concentrations	1223:1251	lower cytokine concentrations	1223:1251	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	0	14	theme	pro-inflammatory	104:119	arg1	cytokines					121:129	pro-inflammatory cytokines	104:129	pro-inflammatory cytokines	104:129	Chondrocyte and mesenchymal stem cell derived engineered cartilage exhibits differential sensitivity to pro-inflammatory cytokines.					
29809295	3	15	theme	engineered	547:556	arg1	cartilage					558:566	engineered cartilage	547:566	engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs)	547:633	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	2	16	theme	inflammatory	308:319	arg1	environment					321:331	the inflammatory environment	304:331	the inflammatory environment of the damaged joint	304:352	However, the inflammatory environment of the damaged joint might compromise outcomes, and this may be impacted by the choice of cell source in terms of their ability to operate anabolically in an inflamed environment.					
29809295	3	17	contain	possessed	708:716	arg1	type					703:706	either cell type	691:706	either cell type	691:706	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	3	17	contain	possessed	708:716	arg2	advantage					730:738	an inherent advantage	718:738	an inherent advantage	718:738	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	5	18	theme	cytokine	1229:1236	arg1	concentrations					1238:1251	lower cytokine concentrations	1223:1251	lower cytokine concentrations	1223:1251	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	3	19	theme	cell	698:701	arg1	type					703:706	either cell type	691:706	either cell type	691:706	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	5	20	theme	properties	1209:1218	arg1	loss					1172:1175	a greater loss	1162:1175	a greater loss of proteoglycans and functional properties	1162:1218	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	3	21	attach	derived	568:574	arg1	chondrocytes					588:599	native chondrocytes	581:599	native chondrocytes	581:599	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	3	21	attach	derived	568:574	arg1	MSCs					629:632	MSCs	629:632	MSCs	629:632	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	3	21	attach	derived	568:574	arg2	cartilage					558:566	engineered cartilage	547:566	engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs)	547:633	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	3	21	attach	derived	568:574	arg1	cells					622:626	mesenchymal stem cells	605:626	mesenchymal stem cells (MSCs)	605:633	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	1	22	theme	articular	193:201	arg1	defects					213:219	articular cartilage defects	193:219	articular cartilage defects	193:219	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	2	23	theme	ability	453:459	arg1	terms					438:442	terms	438:442	terms of their ability to operate anabolically in an inflamed environment	438:510	However, the inflammatory environment of the damaged joint might compromise outcomes, and this may be impacted by the choice of cell source in terms of their ability to operate anabolically in an inflamed environment.					
29809295	6	24	theme	environment	1412:1422	arg1	likely					1432:1437	likely	1432:1437	likely	1432:1437	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	6	24	theme	environment	1412:1422	arg1	presence					1384:1391	the presence	1380:1391	the presence of an inflammatory environment	1380:1422	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	2	25	theme	joint	348:352	arg1	environment					321:331	the inflammatory environment	304:331	the inflammatory environment of the damaged joint	304:352	However, the inflammatory environment of the damaged joint might compromise outcomes, and this may be impacted by the choice of cell source in terms of their ability to operate anabolically in an inflamed environment.					
29809295	1	26	theme	cartilage	203:211	arg1	defects					213:219	articular cartilage defects	193:219	articular cartilage defects	193:219	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	6	27	theme	in	1453:1454	arg1	success					1461:1467	the in vivo success	1449:1467	the in vivo success of MSC-derived cartilage repair	1449:1499	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	6	28	dep	in	1453:1454	arg1	vivo					1456:1459	vivo	1456:1459	vivo	1456:1459	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	2	29	theme	damaged	340:346	arg1	joint					348:352	the damaged joint	336:352	the damaged joint	336:352	However, the inflammatory environment of the damaged joint might compromise outcomes, and this may be impacted by the choice of cell source in terms of their ability to operate anabolically in an inflamed environment.					
29809295	1	30	theme	defects	213:219	arg1	repair					183:188	the repair	179:188	the repair of articular cartilage defects	179:219	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	0	31	theme	stem	28:31	arg1	cell					33:36	mesenchymal stem cell	16:36	mesenchymal stem cell	16:36	Chondrocyte and mesenchymal stem cell derived engineered cartilage exhibits differential sensitivity to pro-inflammatory cytokines.					
29809295	5	32	theme	proteoglycans	1180:1192	arg1	loss					1172:1175	a greater loss	1162:1175	a greater loss of proteoglycans and functional properties	1162:1218	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	4	33	theme	nitric	904:909	arg1	oxide					911:915	nitric oxide	904:915	nitric oxide [NO	904:919	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	3	34	theme	cartilage	558:566	arg1	response					535:542	the response	531:542	the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge	531:646	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	0	35	theme	mesenchymal	16:26	arg1	cell					33:36	mesenchymal stem cell	16:36	mesenchymal stem cell	16:36	Chondrocyte and mesenchymal stem cell derived engineered cartilage exhibits differential sensitivity to pro-inflammatory cytokines.					
29809295	3	36	theme	inherent	721:728	arg1	advantage					730:738	an inherent advantage	718:738	an inherent advantage	718:738	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	7	37	theme	2018	1504:1507	arg1	Research					1521:1528	© 2018 Orthopaedic Research	1502:1528	© 2018 Orthopaedic Research Society.	1502:1537	© 2018 Orthopaedic Research Society.					
29809295	5	38	theme	MSC-derived	1045:1055	arg1	constructs					1057:1066	MSC-derived constructs	1045:1066	MSC-derived constructs	1045:1066	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	1	39	theme	Tissue	132:137	arg1	engineering					139:149	Tissue engineering	132:149	Tissue engineering	132:149	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	1	39	theme	Tissue	132:137	arg1	approach					166:173	a promising approach	154:173	a promising approach for the repair of articular cartilage defects	154:219	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	2	40	theme	source	428:433	arg1	choice					413:418	the choice	409:418	the choice of cell source in terms of their ability to operate anabolically in an inflamed environment	409:510	However, the inflammatory environment of the damaged joint might compromise outcomes, and this may be impacted by the choice of cell source in terms of their ability to operate anabolically in an inflamed environment.					
29809295	5	41	theme	functional	1198:1207	arg1	properties					1209:1218	functional properties	1198:1218	functional properties	1198:1218	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	1	42	theme	engineered	227:236	arg1	constructs					238:247	engineered constructs	227:247	engineered constructs emerging that match native tissue properties	227:292	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	3	43	theme	mesenchymal	605:615	arg1	MSCs					629:632	MSCs	629:632	MSCs	629:632	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	3	43	theme	mesenchymal	605:615	arg1	cells					622:626	mesenchymal stem cells	605:626	mesenchymal stem cells (MSCs)	605:633	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	5	44	link	chondrocyte-derived	1119:1137	arg1	constructs					1139:1148	chondrocyte-derived constructs	1119:1148	chondrocyte-derived constructs	1119:1148	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	2	45	theme	cell	423:426	arg1	source					428:433	cell source	423:433	cell source	423:433	However, the inflammatory environment of the damaged joint might compromise outcomes, and this may be impacted by the choice of cell source in terms of their ability to operate anabolically in an inflamed environment.					
29809295	4	46	theme	tissue-level	960:971	arg1	changes					973:979	cell- and tissue-level changes	950:979	cell- and tissue-level changes	950:979	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	4	47	theme	oxide	911:915	arg1	[NO					917:919	nitric oxide [NO	904:919	nitric oxide [NO	904:919	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	3	48	theme	stem	617:620	arg1	MSCs					629:632	MSCs	629:632	MSCs	629:632	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	3	48	theme	stem	617:620	arg1	cells					622:626	mesenchymal stem cells	605:626	mesenchymal stem cells (MSCs)	605:633	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	4	49	theme	metalloproteinases	877:894	arg1	[MMPs					896:900	matrix metalloproteinases [MMPs	870:900	matrix metalloproteinases [MMPs	870:900	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	4	49	theme	metalloproteinases	877:894	arg1	mediators					859:867	catabolic mediators	849:867	catabolic mediators (matrix metalloproteinases [MMPs]	849:901	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	5	50	theme	inflammatory	1091:1102	arg1	mediators					1104:1112	inflammatory mediators	1091:1112	inflammatory mediators	1091:1112	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	4	51	dep	Compositional	741:753	arg1	mechanical					794:803	mechanical	794:803	mechanical	794:803	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	4	51	dep	Compositional	741:753	arg1	matrix					770:775	extracellular matrix	756:775	extracellular matrix	756:775	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	0	52	theme	engineered	46:55	arg1	cartilage					57:65	engineered cartilage	46:65	engineered cartilage	46:65	Chondrocyte and mesenchymal stem cell derived engineered cartilage exhibits differential sensitivity to pro-inflammatory cytokines.					
29809295	5	53	theme	greater	1164:1170	arg1	loss					1172:1175	a greater loss	1162:1175	a greater loss of proteoglycans and functional properties	1162:1218	Results demonstrated that MSC-derived constructs were more sensitive to inflammatory mediators than chondrocyte-derived constructs, exhibiting a greater loss of proteoglycans and functional properties at lower cytokine concentrations.					
29809295	4	54	theme	matrix	870:875	arg1	metalloproteinases					877:894	matrix metalloproteinases	870:894	matrix metalloproteinases [MMPs	870:900	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	2	55	from	choice	413:418	arg1	terms					438:442	terms	438:442	terms of their ability to operate anabolically in an inflamed environment	438:510	However, the inflammatory environment of the damaged joint might compromise outcomes, and this may be impacted by the choice of cell source in terms of their ability to operate anabolically in an inflamed environment.					
29809295	6	56	theme	engineered	1324:1333	arg1	cartilage					1335:1343	functional engineered cartilage	1313:1343	functional engineered cartilage	1313:1343	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	4	57	theme	functional	782:791	arg1	characteristics					806:820	Compositional (extracellular matrix) and functional (mechanical) characteristics	741:820	Compositional (extracellular matrix) and functional (mechanical) characteristics	741:820	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	4	58	theme	Compositional	741:753	arg1	characteristics					806:820	Compositional (extracellular matrix) and functional (mechanical) characteristics	741:820	Compositional (extracellular matrix) and functional (mechanical) characteristics	741:820	Compositional (extracellular matrix) and functional (mechanical) characteristics, as well as the release of catabolic mediators (matrix metalloproteinases [MMPs], nitric oxide [NO]) were assessed to determine cell- and tissue-level changes following exposure to IL-1β or TNF-α.					
29809295	6	59	link	MSC-derived	1472:1482	arg1	repair					1494:1499	MSC-derived cartilage repair	1472:1499	MSC-derived cartilage repair	1472:1499	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	6	60	contain	have	1287:1290	arg2	capacity					1296:1303	the capacity to form functional engineered cartilage in vitro	1292:1352	the capacity to form functional engineered cartilage in vitro	1292:1352	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	6	60	contain	have	1287:1290	arg1	chondrocytes					1269:1280	chondrocytes	1269:1280	chondrocytes	1269:1280	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	6	60	contain	have	1287:1290	arg1	MSCs					1260:1263	MSCs	1260:1263	MSCs	1260:1263	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	1	61	theme	promising	156:164	arg1	engineering					139:149	Tissue engineering	132:149	Tissue engineering	132:149	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	1	61	theme	promising	156:164	arg1	approach					166:173	a promising approach	154:173	a promising approach for the repair of articular cartilage defects	154:219	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
29809295	0	62	theme	differential	76:87	arg1	sensitivity					89:99	differential sensitivity	76:99	differential sensitivity to pro-inflammatory cytokines	76:129	Chondrocyte and mesenchymal stem cell derived engineered cartilage exhibits differential sensitivity to pro-inflammatory cytokines.					
29809295	6	63	theme	repair	1494:1499	arg1	success					1461:1467	the in vivo success	1449:1467	the in vivo success of MSC-derived cartilage repair	1449:1499	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	6	64	theme	inflammatory	1399:1410	arg1	environment					1412:1422	an inflammatory environment	1396:1422	an inflammatory environment	1396:1422	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	7	65	theme	©	1502:1502	arg1	Research					1521:1528	© 2018 Orthopaedic Research	1502:1528	© 2018 Orthopaedic Research Society.	1502:1537	© 2018 Orthopaedic Research Society.					
29809295	3	66	theme	native	581:586	arg1	chondrocytes					588:599	native chondrocytes	581:599	native chondrocytes	581:599	Here, we compared the response of engineered cartilage derived from native chondrocytes and mesenchymal stem cells (MSCs) to challenge by TNFα and IL-1β in order to determine if either cell type possessed an inherent advantage.					
29809295	6	67	theme	cartilage	1484:1492	arg1	repair					1494:1499	MSC-derived cartilage repair	1472:1499	MSC-derived cartilage repair	1472:1499	While MSCs and chondrocytes both have the capacity to form functional engineered cartilage in vitro, this study suggests that the presence of an inflammatory environment is more likely to impair the in vivo success of MSC-derived cartilage repair.					
29809295	1	68	theme	native	269:274	arg1	properties					283:292	native tissue properties	269:292	native tissue properties	269:292	Tissue engineering is a promising approach for the repair of articular cartilage defects, with engineered constructs emerging that match native tissue properties.					
31198130	5	0	theme	lipoproteins	736:747	arg1	diversity					706:714	a dizzying diversity	695:714	a dizzying diversity of abundant surface lipoproteins instead	695:755	Treponema and Borrelia outer membranes lack lipopolysaccharide, with treponemes expressing only a few outer membrane proteins and Borrelia displaying a dizzying diversity of abundant surface lipoproteins instead.					
31198130	11	1	theme	sorting	1669:1675	arg1	studies					1677:1683	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies	1598:1683	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies	1598:1683	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	2	2	theme	Distinctive	160:170	arg1	features					179:186	Distinctive common features	160:186	Distinctive common features of Borrelia, Leptospira, and Treponema	160:225	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	5	3	theme	surface	728:734	arg1	lipoproteins					736:747	abundant surface lipoproteins	719:747	abundant surface lipoproteins instead	719:755	Treponema and Borrelia outer membranes lack lipopolysaccharide, with treponemes expressing only a few outer membrane proteins and Borrelia displaying a dizzying diversity of abundant surface lipoproteins instead.					
31198130	11	4	theme	detailed	1652:1659	arg1	sorting					1669:1675	detailed protein sorting	1652:1675	detailed protein sorting	1652:1675	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	2	5	theme	thin	286:289	arg1	walls					310:314	thin peptidoglycan cell walls	286:314	thin peptidoglycan cell walls	286:314	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	11	6	theme	lipoprotein	1695:1705	arg1	pathway					1717:1723	a lipoprotein secretion pathway	1693:1723	a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether	1693:1800	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	8	7	theme	flagellar	1283:1291	arg1	proteins					1293:1300	flagellar proteins	1283:1300	flagellar proteins only	1283:1305	Classical type I to III secretion systems have been identified, with demonstrated roles in drug efflux and export of flagellar proteins only.					
31198130	7	8	from	variations	1096:1105	arg1	pathways					1120:1127	secretion pathways	1110:1127	secretion pathways in the periplasm	1110:1144	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	7	8	from	variations	1096:1105	arg1	membrane					1156:1163	outer membrane	1150:1163	outer membrane	1150:1163	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	5	9	theme	dizzying	697:704	arg1	diversity					706:714	a dizzying diversity	695:714	a dizzying diversity of abundant surface lipoproteins instead	695:755	Treponema and Borrelia outer membranes lack lipopolysaccharide, with treponemes expressing only a few outer membrane proteins and Borrelia displaying a dizzying diversity of abundant surface lipoproteins instead.					
31198130	10	10	theme	Lol	1528:1530	arg1	pathways					1532:1539	functional Lol pathways	1517:1539	functional Lol pathways that lack the outer membrane lipoprotein insertase LolB	1517:1595	Proper lipoprotein sorting within the periplasm appears to be dependent on functional Lol pathways that lack the outer membrane lipoprotein insertase LolB.					
31198130	9	11	theme	periplasmic	1329:1339	arg1	protease					1375:1382	a C-terminal protease	1362:1382	a C-terminal protease	1362:1382	Unique activities of periplasmic proteases, including a C-terminal protease, are involved in maturation of some periplasmic proteins.					
31198130	9	11	theme	periplasmic	1329:1339	arg1	proteases					1341:1349	periplasmic proteases	1329:1349	periplasmic proteases	1329:1349	Unique activities of periplasmic proteases, including a C-terminal protease, are involved in maturation of some periplasmic proteins.					
31198130	2	12	theme	cell	305:308	arg1	walls					310:314	thin peptidoglycan cell walls	286:314	thin peptidoglycan cell walls	286:314	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	2	13	theme	Treponema	217:225	arg1	features					179:186	Distinctive common features	160:186	Distinctive common features of Borrelia, Leptospira, and Treponema	160:225	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	7	14	theme	secretion	1110:1118	arg1	pathways					1120:1127	secretion pathways	1110:1127	secretion pathways in the periplasm	1110:1144	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	1	15	theme	unusual	114:120	arg1	morphologies					122:133	unusual morphologies	114:133	unusual morphologies	114:133	Spirochetes form a separate phylum of bacteria with two membranes but otherwise unusual morphologies and envelope structures.					
31198130	12	16	theme	outer	1842:1846	arg1	vesicles					1857:1864	outer membrane vesicles	1842:1864	outer membrane vesicles	1842:1864	Proteins can be released from cells in outer membrane vesicles or, rarely, as soluble proteins.					
31198130	11	17	theme	surface	1615:1621	arg1	lipoproteins					1623:1634	surface lipoproteins	1615:1634	surface lipoproteins	1615:1634	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	11	18	theme	abundance	1602:1610	arg1	studies					1677:1683	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies	1598:1683	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies	1598:1683	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	4	19	theme	lipopolysaccharides	524:542	arg1	incorporation					507:519	the incorporation	503:519	the incorporation of lipopolysaccharides	503:542	Leptospira most closely track Gram-negative bacteria due to the incorporation of lipopolysaccharides.					
31198130	10	20	theme	membrane	1561:1568	arg1	LolB					1592:1595	the outer membrane lipoprotein insertase LolB	1551:1595	the outer membrane lipoprotein insertase LolB	1551:1595	Proper lipoprotein sorting within the periplasm appears to be dependent on functional Lol pathways that lack the outer membrane lipoprotein insertase LolB.					
31198130	5	21	theme	outer	647:651	arg1	proteins					662:669	only a few outer membrane proteins	636:669	only a few outer membrane proteins	636:669	Treponema and Borrelia outer membranes lack lipopolysaccharide, with treponemes expressing only a few outer membrane proteins and Borrelia displaying a dizzying diversity of abundant surface lipoproteins instead.					
31198130	9	22	theme	periplasmic	1420:1430	arg1	proteins					1432:1439	some periplasmic proteins	1415:1439	some periplasmic proteins	1415:1439	Unique activities of periplasmic proteases, including a C-terminal protease, are involved in maturation of some periplasmic proteins.					
31198130	7	23	theme	various	1044:1050	arg1	architectures					1061:1073	various envelope architectures	1044:1073	various envelope architectures	1044:1073	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	1	24	theme	bacteria	72:79	arg1	phylum					62:67	a separate phylum	51:67	a separate phylum of bacteria with two membranes but otherwise unusual morphologies and envelope structures	51:157	Spirochetes form a separate phylum of bacteria with two membranes but otherwise unusual morphologies and envelope structures.					
31198130	10	25	theme	insertase	1582:1590	arg1	LolB					1592:1595	the outer membrane lipoprotein insertase LolB	1551:1595	the outer membrane lipoprotein insertase LolB	1551:1595	Proper lipoprotein sorting within the periplasm appears to be dependent on functional Lol pathways that lack the outer membrane lipoprotein insertase LolB.					
31198130	0	26	theme	Protein	0:6	arg1	Secretion					8:16	Protein Secretion	0:16	Protein Secretion in Spirochetes.	0:32	Protein Secretion in Spirochetes.					
31198130	8	27	theme	type	1176:1179	arg1	systems					1200:1206	Classical type I to III secretion systems	1166:1206	Classical type I to III secretion systems	1166:1206	Classical type I to III secretion systems have been identified, with demonstrated roles in drug efflux and export of flagellar proteins only.					
31198130	11	28	theme	secretion	1707:1715	arg1	pathway					1717:1723	a lipoprotein secretion pathway	1693:1723	a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether	1693:1800	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	3	29	theme	Outer	374:378	arg1	compositions					389:400	Outer membrane compositions	374:400	Outer membrane compositions	374:400	Outer membrane compositions differ significantly between the genera.					
31198130	8	30	theme	secretion	1190:1198	arg1	systems					1200:1206	Classical type I to III secretion systems	1166:1206	Classical type I to III secretion systems	1166:1206	Classical type I to III secretion systems have been identified, with demonstrated roles in drug efflux and export of flagellar proteins only.					
31198130	2	31	theme	common	172:177	arg1	features					179:186	Distinctive common features	160:186	Distinctive common features of Borrelia, Leptospira, and Treponema	160:225	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	10	32	theme	lipoprotein	1449:1459	arg1	sorting					1461:1467	Proper lipoprotein sorting	1442:1467	Proper lipoprotein sorting within the periplasm	1442:1488	Proper lipoprotein sorting within the periplasm appears to be dependent on functional Lol pathways that lack the outer membrane lipoprotein insertase LolB.					
31198130	6	33	theme	pathways	887:894	arg1	modules					845:851	various modules	837:851	various modules of bacterial export and secretion pathways	837:894	Phylogenetic and experimental evidence indicates that spirochetes have adapted various modules of bacterial export and secretion pathways to build and maintain their envelopes.					
31198130	6	34	theme	various	837:843	arg1	modules					845:851	various modules	837:851	various modules of bacterial export and secretion pathways	837:894	Phylogenetic and experimental evidence indicates that spirochetes have adapted various modules of bacterial export and secretion pathways to build and maintain their envelopes.					
31198130	7	35	theme	spirochetal	1011:1021	arg1	experimentation					1023:1037	spirochetal experimentation	1011:1037	spirochetal experimentation with various envelope architectures over time	1011:1083	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	7	36	from	pathways	956:963	arg1	membrane					978:985	the inner membrane	968:985	the inner membrane	968:985	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	11	37	theme	outer	1761:1765	arg1	membrane					1767:1774	the outer membrane	1757:1774	the outer membrane	1757:1774	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	7	38	from	membrane	1156:1163	arg1	periplasm					1136:1144	the periplasm	1132:1144	the periplasm	1132:1144	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	2	39	theme	inner	358:362	arg1	membrane					364:371	the inner membrane	354:371	the inner membrane	354:371	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	12	40	theme	soluble	1881:1887	arg1	proteins					1889:1896	soluble proteins	1881:1896	soluble proteins	1881:1896	Proteins can be released from cells in outer membrane vesicles or, rarely, as soluble proteins.					
31198130	6	41	theme	secretion	877:885	arg1	pathways					887:894	bacterial export and secretion pathways	856:894	bacterial export and secretion pathways	856:894	Phylogenetic and experimental evidence indicates that spirochetes have adapted various modules of bacterial export and secretion pathways to build and maintain their envelopes.					
31198130	5	42	theme	abundant	719:726	arg1	lipoproteins					736:747	abundant surface lipoproteins	719:747	abundant surface lipoproteins instead	719:755	Treponema and Borrelia outer membranes lack lipopolysaccharide, with treponemes expressing only a few outer membrane proteins and Borrelia displaying a dizzying diversity of abundant surface lipoproteins instead.					
31198130	2	43	theme	peptidoglycan	291:303	arg1	walls					310:314	thin peptidoglycan cell walls	286:314	thin peptidoglycan cell walls	286:314	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	7	44	theme	inner	972:976	arg1	membrane					978:985	the inner membrane	968:985	the inner membrane	968:985	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	8	45	theme	drug	1257:1260	arg1	efflux					1262:1267	drug efflux	1257:1267	drug efflux	1257:1267	Classical type I to III secretion systems have been identified, with demonstrated roles in drug efflux and export of flagellar proteins only.					
31198130	11	46	theme	protein	1661:1667	arg1	sorting					1669:1675	detailed protein sorting	1652:1675	detailed protein sorting	1652:1675	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	9	47	theme	Unique	1308:1313	arg1	activities					1315:1324	Unique activities	1308:1324	Unique activities of periplasmic proteases, including a C-terminal protease,	1308:1383	Unique activities of periplasmic proteases, including a C-terminal protease, are involved in maturation of some periplasmic proteins.					
31198130	7	48	theme	insertion	946:954	arg1	pathways					956:963	Export and insertion pathways	935:963	Export and insertion pathways in the inner membrane	935:985	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	7	49	with	experimentation	1023:1037	arg1	architectures					1061:1073	various envelope architectures	1044:1073	various envelope architectures	1044:1073	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	6	50	theme	export	866:871	arg1	pathways					887:894	bacterial export and secretion pathways	856:894	bacterial export and secretion pathways	856:894	Phylogenetic and experimental evidence indicates that spirochetes have adapted various modules of bacterial export and secretion pathways to build and maintain their envelopes.					
31198130	2	51	theme	walls	310:314	arg1	sequestration					239:251	the sequestration	235:251	the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane	235:371	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	7	52	theme	Export	935:940	arg1	pathways					956:963	Export and insertion pathways	935:963	Export and insertion pathways in the inner membrane	935:985	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	8	53	theme	proteins	1293:1300	arg1	roles					1248:1252	roles	1248:1252	roles in drug efflux and export of flagellar proteins only	1248:1305	Classical type I to III secretion systems have been identified, with demonstrated roles in drug efflux and export of flagellar proteins only.					
31198130	4	54	theme	Gram-negative	473:485	arg1	bacteria					487:494	Gram-negative bacteria	473:494	Gram-negative bacteria due to the incorporation of lipopolysaccharides	473:542	Leptospira most closely track Gram-negative bacteria due to the incorporation of lipopolysaccharides.					
31198130	7	55	from	pathways	1120:1127	arg1	periplasm					1136:1144	the periplasm	1132:1144	the periplasm	1132:1144	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	9	56	theme	proteases	1341:1349	arg1	activities					1315:1324	Unique activities	1308:1324	Unique activities of periplasmic proteases, including a C-terminal protease,	1308:1383	Unique activities of periplasmic proteases, including a C-terminal protease, are involved in maturation of some periplasmic proteins.					
31198130	4	57	theme	due	496:498	arg1	bacteria					487:494	Gram-negative bacteria	473:494	Gram-negative bacteria due to the incorporation of lipopolysaccharides	473:542	Leptospira most closely track Gram-negative bacteria due to the incorporation of lipopolysaccharides.					
31198130	5	58	dep	Treponema	545:553	arg1	membranes					574:582	outer membranes	568:582	outer membranes	568:582	Treponema and Borrelia outer membranes lack lipopolysaccharide, with treponemes expressing only a few outer membrane proteins and Borrelia displaying a dizzying diversity of abundant surface lipoproteins instead.					
31198130	7	59	theme	outer	1150:1154	arg1	membrane					1156:1163	outer membrane	1150:1163	outer membrane	1150:1163	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	1	60	with	phylum	62:67	arg1	morphologies					122:133	unusual morphologies	114:133	unusual morphologies	114:133	Spirochetes form a separate phylum of bacteria with two membranes but otherwise unusual morphologies and envelope structures.					
31198130	1	60	with	phylum	62:67	arg1	structures					148:157	envelope structures	139:157	envelope structures	139:157	Spirochetes form a separate phylum of bacteria with two membranes but otherwise unusual morphologies and envelope structures.					
31198130	1	60	with	phylum	62:67	arg1	membranes					90:98	two membranes	86:98	two membranes	86:98	Spirochetes form a separate phylum of bacteria with two membranes but otherwise unusual morphologies and envelope structures.					
31198130	12	61	attach	released	1819:1826	arg2	Proteins					1803:1810	Proteins	1803:1810	Proteins	1803:1810	Proteins can be released from cells in outer membrane vesicles or, rarely, as soluble proteins.					
31198130	12	61	attach	released	1819:1826	arg1	cells					1833:1837	cells	1833:1837	cells	1833:1837	Proteins can be released from cells in outer membrane vesicles or, rarely, as soluble proteins.					
31198130	10	62	theme	functional	1517:1526	arg1	pathways					1532:1539	functional Lol pathways	1517:1539	functional Lol pathways that lack the outer membrane lipoprotein insertase LolB	1517:1595	Proper lipoprotein sorting within the periplasm appears to be dependent on functional Lol pathways that lack the outer membrane lipoprotein insertase LolB.					
31198130	9	63	theme	C-terminal	1364:1373	arg1	protease					1375:1382	a C-terminal protease	1362:1382	a C-terminal protease	1362:1382	Unique activities of periplasmic proteases, including a C-terminal protease, are involved in maturation of some periplasmic proteins.					
31198130	2	64	theme	flagella	256:263	arg1	sequestration					239:251	the sequestration	235:251	the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane	235:371	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	5	65	theme	outer	568:572	arg1	membranes					574:582	outer membranes	568:582	outer membranes	568:582	Treponema and Borrelia outer membranes lack lipopolysaccharide, with treponemes expressing only a few outer membrane proteins and Borrelia displaying a dizzying diversity of abundant surface lipoproteins instead.					
31198130	1	66	theme	envelope	139:146	arg1	structures					148:157	envelope structures	139:157	envelope structures	139:157	Spirochetes form a separate phylum of bacteria with two membranes but otherwise unusual morphologies and envelope structures.					
31198130	12	67	theme	membrane	1848:1855	arg1	vesicles					1857:1864	outer membrane vesicles	1842:1864	outer membrane vesicles	1842:1864	Proteins can be released from cells in outer membrane vesicles or, rarely, as soluble proteins.					
31198130	0	68	from	Secretion	8:16	arg1	Spirochetes					21:31	Spirochetes	21:31	Spirochetes	21:31	Protein Secretion in Spirochetes.					
31198130	10	69	theme	outer	1555:1559	arg1	LolB					1592:1595	the outer membrane lipoprotein insertase LolB	1551:1595	the outer membrane lipoprotein insertase LolB	1551:1595	Proper lipoprotein sorting within the periplasm appears to be dependent on functional Lol pathways that lack the outer membrane lipoprotein insertase LolB.					
31198130	11	70	theme	lipoproteins	1623:1634	arg1	sorting					1669:1675	detailed protein sorting	1652:1675	detailed protein sorting	1652:1675	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	11	70	theme	lipoproteins	1623:1634	arg1	abundance					1602:1610	The abundance	1598:1610	The abundance of surface lipoproteins in Borrelia	1598:1646	The abundance of surface lipoproteins in Borrelia and detailed protein sorting studies suggest a lipoprotein secretion pathway that either extends Lol through the outer membrane or bypasses it altogether.					
31198130	5	71	theme	membrane	653:660	arg1	proteins					662:669	only a few outer membrane proteins	636:669	only a few outer membrane proteins	636:669	Treponema and Borrelia outer membranes lack lipopolysaccharide, with treponemes expressing only a few outer membrane proteins and Borrelia displaying a dizzying diversity of abundant surface lipoproteins instead.					
31198130	1	72	theme	separate	53:60	arg1	phylum					62:67	a separate phylum	51:67	a separate phylum of bacteria with two membranes but otherwise unusual morphologies and envelope structures	51:157	Spirochetes form a separate phylum of bacteria with two membranes but otherwise unusual morphologies and envelope structures.					
31198130	8	73	theme	Classical	1166:1174	arg1	systems					1200:1206	Classical type I to III secretion systems	1166:1206	Classical type I to III secretion systems	1166:1206	Classical type I to III secretion systems have been identified, with demonstrated roles in drug efflux and export of flagellar proteins only.					
31198130	7	74	dep	appear	987:992	arg1	conserved					994:1002	conserved	994:1002	conserved	994:1002	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	6	75	theme	experimental	775:786	arg1	evidence					788:795	Phylogenetic and experimental evidence	758:795	Phylogenetic and experimental evidence	758:795	Phylogenetic and experimental evidence indicates that spirochetes have adapted various modules of bacterial export and secretion pathways to build and maintain their envelopes.					
31198130	9	76	theme	proteins	1432:1439	arg1	maturation					1401:1410	maturation	1401:1410	maturation of some periplasmic proteins	1401:1439	Unique activities of periplasmic proteases, including a C-terminal protease, are involved in maturation of some periplasmic proteins.					
31198130	7	77	theme	envelope	1052:1059	arg1	architectures					1061:1073	various envelope architectures	1044:1073	various envelope architectures	1044:1073	Export and insertion pathways in the inner membrane appear conserved, while spirochetal experimentation with various envelope architectures over time has led to variations in secretion pathways in the periplasm and outer membrane.					
31198130	10	78	theme	lipoprotein	1570:1580	arg1	LolB					1592:1595	the outer membrane lipoprotein insertase LolB	1551:1595	the outer membrane lipoprotein insertase LolB	1551:1595	Proper lipoprotein sorting within the periplasm appears to be dependent on functional Lol pathways that lack the outer membrane lipoprotein insertase LolB.					
31198130	6	79	theme	Phylogenetic	758:769	arg1	evidence					788:795	Phylogenetic and experimental evidence	758:795	Phylogenetic and experimental evidence	758:795	Phylogenetic and experimental evidence indicates that spirochetes have adapted various modules of bacterial export and secretion pathways to build and maintain their envelopes.					
31198130	3	80	theme	membrane	380:387	arg1	compositions					389:400	Outer membrane compositions	374:400	Outer membrane compositions	374:400	Outer membrane compositions differ significantly between the genera.					
31198130	2	81	theme	Borrelia	191:198	arg1	features					179:186	Distinctive common features	160:186	Distinctive common features of Borrelia, Leptospira, and Treponema	160:225	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	8	82	from	roles	1248:1252	arg1	efflux					1262:1267	drug efflux	1257:1267	drug efflux	1257:1267	Classical type I to III secretion systems have been identified, with demonstrated roles in drug efflux and export of flagellar proteins only.					
31198130	8	82	from	roles	1248:1252	arg1	export					1273:1278	export	1273:1278	export	1273:1278	Classical type I to III secretion systems have been identified, with demonstrated roles in drug efflux and export of flagellar proteins only.					
31198130	6	83	theme	bacterial	856:864	arg1	pathways					887:894	bacterial export and secretion pathways	856:894	bacterial export and secretion pathways	856:894	Phylogenetic and experimental evidence indicates that spirochetes have adapted various modules of bacterial export and secretion pathways to build and maintain their envelopes.					
31198130	10	84	theme	Proper	1442:1447	arg1	sorting					1461:1467	Proper lipoprotein sorting	1442:1467	Proper lipoprotein sorting within the periplasm	1442:1488	Proper lipoprotein sorting within the periplasm appears to be dependent on functional Lol pathways that lack the outer membrane lipoprotein insertase LolB.					
31198130	2	85	theme	Leptospira	201:210	arg1	features					179:186	Distinctive common features	160:186	Distinctive common features of Borrelia, Leptospira, and Treponema	160:225	Distinctive common features of Borrelia, Leptospira, and Treponema include the sequestration of flagella to the periplasm and thin peptidoglycan cell walls that are more closely associated with the inner membrane.					
31198130	8	86	dep	type	1176:1179	arg1	to					1183:1184	to	1183:1184	to	1183:1184	Classical type I to III secretion systems have been identified, with demonstrated roles in drug efflux and export of flagellar proteins only.					
29556804	9	0	theme	blot	1570:1573	arg1	analysis					1575:1582	Western blot analysis	1562:1582	Western blot analysis	1562:1582	Western blot analysis showed that the MMP-9 and lysozyme levels in the subcutis were higher than those in the spinal cord and brain (P < 0.05).					
29556804	6	1	theme	transplantation	1063:1077	arg1	requirements					1039:1050	the biological requirements	1024:1050	the biological requirements of in vivo transplantation	1024:1077	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	10	2	contain	has	1778:1780	arg2	biocompatibility					1787:1802	good biocompatibility	1782:1802	good biocompatibility	1782:1802	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	10	2	contain	has	1778:1780	arg2	biodegradability					1808:1823	biodegradability	1808:1823	biodegradability	1808:1823	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	10	2	contain	has	1778:1780	arg1	scaffold					1769:1776	the CG-CS scaffold	1759:1776	the CG-CS scaffold	1759:1776	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	5	3	theme	SD	639:640	arg1	rats					642:645	SD rats	639:645	SD rats	639:645	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	2	4	from	tissues	309:315	arg1	different					287:295	different	287:295	different	287:295	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	2	4	from	tissues	309:315	arg1	rate					263:266	the degradation rate	247:266	the degradation rate of biomaterials	247:282	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	10	5	theme	degradation	1844:1854	arg1	different					1874:1882	different	1874:1882	different	1874:1882	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	10	5	theme	degradation	1844:1854	arg1	rate					1836:1839	the rate	1832:1839	the rate of degradation	1832:1854	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	10	6	dep	in	1721:1722	arg1	vivo					1724:1727	vivo	1724:1727	vivo	1724:1727	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	2	7	theme	degradation	251:261	arg1	different					287:295	different	287:295	different	287:295	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	2	7	theme	degradation	251:261	arg1	rate					263:266	the degradation rate	247:266	the degradation rate of biomaterials	247:282	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	1	8	theme	important	160:168	arg1	index					170:174	an important index	157:174	an important index for evaluating biomaterials	157:202	Degradation rate is an important index for evaluating biomaterials.					
29556804	1	8	theme	important	160:168	arg1	rate					149:152	Degradation rate	137:152	Degradation rate	137:152	Degradation rate is an important index for evaluating biomaterials.					
29556804	6	9	theme	Physical	925:932	arg1	testing					956:962	Physical and chemical property testing	925:962	Physical and chemical property testing	925:962	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	11	10	theme	different	1977:1985	arg1	parts					1987:1991	different parts	1977:1991	different parts of the body	1977:2003	Macrophage behavior and vascularization in different parts of the body may result in control over the balance of degradation and reconstruction.					
29556804	0	11	theme	brain	115:119	arg1	tissues					121:127	brain tissues	115:127	brain tissues	115:127	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	11	12	theme	Macrophage	1934:1943	arg1	behavior					1945:1952	Macrophage behavior	1934:1952	Macrophage behavior	1934:1952	Macrophage behavior and vascularization in different parts of the body may result in control over the balance of degradation and reconstruction.					
29556804	5	13	theme	continuous	688:697	arg1	monitoring					699:708	continuous monitoring	688:708	continuous monitoring of weight loss	688:723	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	11	14	theme	body	2000:2003	arg1	parts					1987:1991	different parts	1977:1991	different parts of the body	1977:2003	Macrophage behavior and vascularization in different parts of the body may result in control over the balance of degradation and reconstruction.					
29556804	7	15	theme	in	1084:1085	arg1	results					1105:1111	The in vivo experimental results	1080:1111	The in vivo experimental results	1080:1111	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	5	16	theme	degradation	660:670	arg1	rate					652:655	the rate	648:655	the rate of degradation	648:670	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	6	17	theme	composite	985:993	arg1	components					1004:1013	CG-CS composite scaffold components	979:1013	CG-CS composite scaffold components	979:1013	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	8	18	theme	cord	1419:1422	arg1	groups					1434:1439	the spinal cord and brain groups	1408:1439	the spinal cord and brain groups	1408:1439	HE staining found that compared with the spinal cord and brain groups, macrophages and capillaries appeared earlier in the subcutis group, and the number was significantly higher (P < 0.05).					
29556804	10	19	theme	same	1917:1920	arg1	point					1927:1931	the same time point	1913:1931	the same time point	1913:1931	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	0	20	theme	collagen/chitosan	60:76	arg1	complex					78:84	a collagen/chitosan complex	58:84	a collagen/chitosan complex	58:84	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	1	21	theme	Degradation	137:147	arg1	index					170:174	an important index	157:174	an important index for evaluating biomaterials	157:202	Degradation rate is an important index for evaluating biomaterials.					
29556804	1	21	theme	Degradation	137:147	arg1	rate					149:152	Degradation rate	137:152	Degradation rate	137:152	Degradation rate is an important index for evaluating biomaterials.					
29556804	2	22	from	different	287:295	arg1	tissues					309:315	distinct tissues	300:315	distinct tissues	300:315	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	9	23	theme	spinal	1672:1677	arg1	cord					1679:1682	the spinal cord	1668:1682	the spinal cord	1668:1682	Western blot analysis showed that the MMP-9 and lysozyme levels in the subcutis were higher than those in the spinal cord and brain (P < 0.05).					
29556804	0	24	from	rate	25:28	arg1	subcutis					89:96	subcutis	89:96	subcutis	89:96	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	0	24	from	rate	25:28	arg1	cord					106:109	spinal cord	99:109	spinal cord	99:109	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	0	24	from	rate	25:28	arg1	tissues					121:127	brain tissues	115:127	brain tissues	115:127	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	11	25	from	vascularization	1958:1972	arg1	parts					1987:1991	different parts	1977:1991	different parts of the body	1977:2003	Macrophage behavior and vascularization in different parts of the body may result in control over the balance of degradation and reconstruction.					
29556804	6	26	theme	chemical	938:945	arg1	testing					956:962	Physical and chemical property testing	925:962	Physical and chemical property testing	925:962	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	5	27	theme	loss	720:723	arg1	monitoring					699:708	continuous monitoring	688:708	continuous monitoring of weight loss	688:723	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	5	28	located	detected	864:871	arg2	MMP-9					839:843	MMP-9	839:843	MMP-9	839:843	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	5	28	located	detected	864:871	arg2	metalloproteinase					818:834	matrix metalloproteinase 9	811:836	matrix metalloproteinase 9 (MMP-9)	811:844	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	5	28	located	detected	864:871	arg2	lysozyme					850:857	lysozyme	850:857	lysozyme	850:857	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	5	28	located	detected	864:871	arg1	stage					886:890	the rapid stage	876:890	the rapid stage of degradation of the scaffolds	876:922	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	4	29	theme	water	483:487	arg1	absorption					489:498	water absorption	483:498	water absorption	483:498	The porosity, water absorption and swelling ratio of the scaffolds were tested in vitro.					
29556804	0	30	theme	Differential	0:11	arg1	rate					25:28	Differential degradation rate	0:28	Differential degradation rate	0:28	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	7	31	from	scaffolds	1196:1204	arg1	groups					1235:1240	the spinal cord and brain groups	1209:1240	the spinal cord and brain groups	1209:1240	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	5	32	theme	degradation	895:905	arg1	stage					886:890	the rapid stage	876:890	the rapid stage of degradation of the scaffolds	876:922	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	6	33	theme	biological	1028:1037	arg1	requirements					1039:1050	the biological requirements	1024:1050	the biological requirements of in vivo transplantation	1024:1077	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	6	34	dep	in	1055:1056	arg1	vivo					1058:1061	vivo	1058:1061	vivo	1058:1061	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	8	35	theme	subcutis	1494:1501	arg1	group					1503:1507	the subcutis group	1490:1507	the subcutis group	1490:1507	HE staining found that compared with the spinal cord and brain groups, macrophages and capillaries appeared earlier in the subcutis group, and the number was significantly higher (P < 0.05).					
29556804	3	36	theme	freeze-drying	443:455	arg1	technology					457:466	freeze-drying technology	443:466	freeze-drying technology	443:466	The collagen-chitosan (CG-CS) composite scaffolds were prepared using freeze-drying technology.					
29556804	5	37	theme	target	754:759	arg1	areas					761:765	target areas	754:765	target areas	754:765	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	0	38	theme	underlying	34:43	arg1	mechanism					45:53	underlying mechanism	34:53	underlying mechanism	34:53	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	7	39	theme	mass	1264:1267	arg1	loss					1269:1272	progressive mass loss	1252:1272	progressive mass loss starting from the 3rd week	1252:1299	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	11	40	theme	degradation	2047:2057	arg1	balance					2036:2042	the balance	2032:2042	the balance of degradation and reconstruction	2032:2076	Macrophage behavior and vascularization in different parts of the body may result in control over the balance of degradation and reconstruction.					
29556804	10	41	theme	good	1782:1785	arg1	biocompatibility					1787:1802	good biocompatibility	1782:1802	good biocompatibility	1782:1802	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	7	42	theme	brain	1229:1233	arg1	groups					1235:1240	the spinal cord and brain groups	1209:1240	the spinal cord and brain groups	1209:1240	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	11	43	from	behavior	1945:1952	arg1	parts					1987:1991	different parts	1977:1991	different parts of the body	1977:2003	Macrophage behavior and vascularization in different parts of the body may result in control over the balance of degradation and reconstruction.					
29556804	9	44	theme	Western	1562:1568	arg1	analysis					1575:1582	Western blot analysis	1562:1582	Western blot analysis	1562:1582	Western blot analysis showed that the MMP-9 and lysozyme levels in the subcutis were higher than those in the spinal cord and brain (P < 0.05).					
29556804	7	45	theme	cord	1220:1223	arg1	groups					1235:1240	the spinal cord and brain groups	1209:1240	the spinal cord and brain groups	1209:1240	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	7	46	theme	experimental	1092:1103	arg1	results					1105:1111	The in vivo experimental results	1080:1111	The in vivo experimental results	1080:1111	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	2	47	theme	underlying	336:345	arg1	mechanism					347:355	the underlying mechanism	332:355	the underlying mechanism of degradation	332:370	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	8	48	theme	brain	1428:1432	arg1	groups					1434:1439	the spinal cord and brain groups	1408:1439	the spinal cord and brain groups	1408:1439	HE staining found that compared with the spinal cord and brain groups, macrophages and capillaries appeared earlier in the subcutis group, and the number was significantly higher (P < 0.05).					
29556804	5	49	theme	rats	642:645	arg1	subcutis					596:603	the subcutis	592:603	the subcutis	592:603	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	5	49	theme	rats	642:645	arg1	cord					613:616	spinal cord	606:616	spinal cord	606:616	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	5	49	theme	rats	642:645	arg1	tissues					628:634	brain tissues	622:634	brain tissues	622:634	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	2	50	theme	biomaterials	271:282	arg1	different					287:295	different	287:295	different	287:295	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	2	50	theme	biomaterials	271:282	arg1	rate					263:266	the degradation rate	247:266	the degradation rate of biomaterials	247:282	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	6	51	theme	in	1055:1056	arg1	transplantation					1063:1077	in vivo transplantation	1055:1077	in vivo transplantation	1055:1077	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	9	52	theme	MMP-9	1600:1604	arg1	higher					1647:1652	higher	1647:1652	higher	1647:1652	Western blot analysis showed that the MMP-9 and lysozyme levels in the subcutis were higher than those in the spinal cord and brain (P < 0.05).					
29556804	9	52	theme	MMP-9	1600:1604	arg1	levels					1619:1624	the MMP-9 and lysozyme levels	1596:1624	levels	1619:1624	Western blot analysis showed that the MMP-9 and lysozyme levels in the subcutis were higher than those in the spinal cord and brain (P < 0.05).					
29556804	7	53	dep	in	1084:1085	arg1	vivo					1087:1090	vivo	1087:1090	vivo	1087:1090	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	5	54	theme	brain	622:626	arg1	tissues					628:634	brain tissues	622:634	brain tissues	622:634	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	2	55	theme	distinct	300:307	arg1	tissues					309:315	distinct tissues	300:315	distinct tissues	300:315	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	9	56	theme	lysozyme	1610:1617	arg1	higher					1647:1652	higher	1647:1652	higher	1647:1652	Western blot analysis showed that the MMP-9 and lysozyme levels in the subcutis were higher than those in the spinal cord and brain (P < 0.05).					
29556804	9	56	theme	lysozyme	1610:1617	arg1	levels					1619:1624	the MMP-9 and lysozyme levels	1596:1624	levels	1619:1624	Western blot analysis showed that the MMP-9 and lysozyme levels in the subcutis were higher than those in the spinal cord and brain (P < 0.05).					
29556804	1	57	theme	evaluating	180:189	arg1	biomaterials					191:202	evaluating biomaterials	180:202	evaluating biomaterials	180:202	Degradation rate is an important index for evaluating biomaterials.					
29556804	0	58	theme	rat	132:134	arg1	subcutis					89:96	subcutis	89:96	subcutis	89:96	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	0	58	theme	rat	132:134	arg1	cord					106:109	spinal cord	99:109	spinal cord	99:109	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	0	58	theme	rat	132:134	arg1	tissues					121:127	brain tissues	115:127	brain tissues	115:127	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	5	59	theme	matrix	811:816	arg1	metalloproteinase					818:834	matrix metalloproteinase 9	811:836	matrix metalloproteinase 9 (MMP-9)	811:844	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	5	59	theme	matrix	811:816	arg1	MMP-9					839:843	MMP-9	839:843	MMP-9	839:843	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	7	60	theme	scaffold	1336:1343	arg1	fraction					1320:1327	a substantial fraction	1306:1327	a substantial fraction of the scaffold	1306:1343	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	8	61	theme	spinal	1412:1417	arg1	cord					1419:1422	the spinal cord	1408:1422	the spinal cord	1408:1422	HE staining found that compared with the spinal cord and brain groups, macrophages and capillaries appeared earlier in the subcutis group, and the number was significantly higher (P < 0.05).					
29556804	10	62	theme	experiments	1729:1739	arg1	results					1710:1716	The results	1706:1716	The results of in vivo experiments	1706:1739	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	5	63	theme	rapid	880:884	arg1	stage					886:890	the rapid stage	876:890	the rapid stage of degradation of the scaffolds	876:922	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	6	64	theme	scaffold	995:1002	arg1	components					1004:1013	CG-CS composite scaffold components	979:1013	CG-CS composite scaffold components	979:1013	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	7	65	theme	substantial	1308:1318	arg1	fraction					1320:1327	a substantial fraction	1306:1327	a substantial fraction of the scaffold	1306:1343	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	10	66	theme	time	1922:1925	arg1	point					1927:1931	the same time point	1913:1931	the same time point	1913:1931	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	0	67	theme	complex	78:84	arg1	rate					25:28	Differential degradation rate	0:28	Differential degradation rate	0:28	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	0	67	theme	complex	78:84	arg1	mechanism					45:53	underlying mechanism	34:53	underlying mechanism	34:53	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	10	68	theme	in	1721:1722	arg1	experiments					1729:1739	in vivo experiments	1721:1739	in vivo experiments	1721:1739	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	6	69	theme	CG-CS	979:983	arg1	components					1004:1013	CG-CS composite scaffold components	979:1013	CG-CS composite scaffold components	979:1013	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	9	70	from	levels	1619:1624	arg1	subcutis					1633:1640	the subcutis	1629:1640	the subcutis	1629:1640	Western blot analysis showed that the MMP-9 and lysozyme levels in the subcutis were higher than those in the spinal cord and brain (P < 0.05).					
29556804	4	71	theme	swelling	504:511	arg1	ratio					513:517	swelling ratio	504:517	swelling ratio	504:517	The porosity, water absorption and swelling ratio of the scaffolds were tested in vitro.					
29556804	5	72	theme	pathological	730:741	arg1	changes					743:749	the pathological changes	726:749	the pathological changes of target areas	726:765	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	0	73	theme	spinal	99:104	arg1	cord					106:109	spinal cord	99:109	spinal cord	99:109	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	5	74	theme	scaffolds	914:922	arg1	degradation					895:905	degradation	895:905	degradation of the scaffolds	895:922	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	6	75	theme	property	947:954	arg1	testing					956:962	Physical and chemical property testing	925:962	Physical and chemical property testing	925:962	Physical and chemical property testing confirmed that CG-CS composite scaffold components can meet the biological requirements of in vivo transplantation.					
29556804	5	76	theme	weight	713:718	arg1	loss					720:723	weight loss	713:723	weight loss	713:723	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	8	77	dep	higher	1543:1548	arg1	P < 0.05					1551:1558	P < 0.05	1551:1558	P < 0.05	1551:1558	HE staining found that compared with the spinal cord and brain groups, macrophages and capillaries appeared earlier in the subcutis group, and the number was significantly higher (P < 0.05).					
29556804	5	78	theme	spinal	606:611	arg1	cord					613:616	spinal cord	606:616	spinal cord	606:616	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	7	79	theme	3rd	1292:1294	arg1	week					1296:1299	the 3rd week	1288:1299	the 3rd week	1288:1299	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	0	80	from	mechanism	45:53	arg1	subcutis					89:96	subcutis	89:96	subcutis	89:96	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	0	80	from	mechanism	45:53	arg1	cord					106:109	spinal cord	99:109	spinal cord	99:109	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	0	80	from	mechanism	45:53	arg1	tissues					121:127	brain tissues	115:127	brain tissues	115:127	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	0	81	theme	degradation	13:23	arg1	rate					25:28	Differential degradation rate	0:28	Differential degradation rate	0:28	Differential degradation rate and underlying mechanism of a collagen/chitosan complex in subcutis, spinal cord and brain tissues of rat.					
29556804	3	82	theme	collagen-chitosan	377:393	arg1	scaffolds					413:421	The collagen-chitosan (CG-CS) composite scaffolds	373:421	The collagen-chitosan (CG-CS) composite scaffolds	373:421	The collagen-chitosan (CG-CS) composite scaffolds were prepared using freeze-drying technology.					
29556804	2	83	theme	degradation	360:370	arg1	mechanism					347:355	the underlying mechanism	332:355	the underlying mechanism of degradation	332:370	The authors' aim was to determine whether the degradation rate of biomaterials is different in distinct tissues and to clarify the underlying mechanism of degradation.					
29556804	5	84	theme	histological	784:795	arg1	staining					797:804	histological staining	784:804	histological staining	784:804	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	11	85	theme	reconstruction	2063:2076	arg1	balance					2036:2042	the balance	2032:2042	the balance of degradation and reconstruction	2032:2076	Macrophage behavior and vascularization in different parts of the body may result in control over the balance of degradation and reconstruction.					
29556804	10	86	theme	CG-CS	1763:1767	arg1	scaffold					1769:1776	the CG-CS scaffold	1759:1776	the CG-CS scaffold	1759:1776	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	10	87	from	point	1927:1931	arg1	different					1874:1882	different	1874:1882	different	1874:1882	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	10	87	from	point	1927:1931	arg1	rate					1836:1839	the rate	1832:1839	the rate of degradation	1832:1854	The results of in vivo experiments demonstrated that the CG-CS scaffold has good biocompatibility and biodegradability, while the rate of degradation was significantly different between the three tissues at the same time point.					
29556804	7	88	theme	progressive	1252:1262	arg1	loss					1269:1272	progressive mass loss	1252:1272	progressive mass loss starting from the 3rd week	1252:1299	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	3	89	theme	composite	403:411	arg1	scaffolds					413:421	The collagen-chitosan (CG-CS) composite scaffolds	373:421	The collagen-chitosan (CG-CS) composite scaffolds	373:421	The collagen-chitosan (CG-CS) composite scaffolds were prepared using freeze-drying technology.					
29556804	4	90	theme	scaffolds	526:534	arg1	porosity					473:480	The porosity	469:480	The porosity	469:480	The porosity, water absorption and swelling ratio of the scaffolds were tested in vitro.					
29556804	4	90	theme	scaffolds	526:534	arg1	absorption					489:498	water absorption	483:498	water absorption	483:498	The porosity, water absorption and swelling ratio of the scaffolds were tested in vitro.					
29556804	4	90	theme	scaffolds	526:534	arg1	ratio					513:517	swelling ratio	504:517	swelling ratio	504:517	The porosity, water absorption and swelling ratio of the scaffolds were tested in vitro.					
29556804	5	91	theme	areas	761:765	arg1	changes					743:749	the pathological changes	726:749	the pathological changes of target areas	726:765	The scaffolds were implanted into the subcutis, spinal cord and brain tissues of SD rats, the rate of degradation was assessed by continuous monitoring of weight loss, the pathological changes of target areas were observed by histological staining, and matrix metalloproteinase 9 (MMP-9) and lysozyme were detected at the rapid stage of degradation of the scaffolds.					
29556804	3	92	theme	CG-CS	396:400	arg1	scaffolds					413:421	The collagen-chitosan (CG-CS) composite scaffolds	373:421	The collagen-chitosan (CG-CS) composite scaffolds	373:421	The collagen-chitosan (CG-CS) composite scaffolds were prepared using freeze-drying technology.					
29556804	7	93	theme	spinal	1213:1218	arg1	cord					1220:1223	the spinal cord	1209:1223	the spinal cord	1209:1223	The in vivo experimental results showed that the scaffolds were completely absorbed in the subcutis at 12 days, the scaffolds in the spinal cord and brain groups exhibited progressive mass loss starting from the 3rd week, and a substantial fraction of the scaffold was degraded at 12 weeks.					
29556804	8	94	theme	HE	1371:1372	arg1	staining					1374:1381	HE staining	1371:1381	HE staining	1371:1381	HE staining found that compared with the spinal cord and brain groups, macrophages and capillaries appeared earlier in the subcutis group, and the number was significantly higher (P < 0.05).					
31013736	1	0	theme	tobacco	168:174	arg1	particles					194:202	tobacco mosaic virus-like particles	168:202	tobacco mosaic virus-like particles (TMV VLPs)	168:213	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	1	0	theme	tobacco	168:174	arg1	VLPs					209:212	TMV VLPs	205:212	TMV VLPs	205:212	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	2	1	theme	mixing	448:453	arg1	step					455:458	a simple mixing step	439:458	a simple mixing step	439:458	Moreover, we showed that the TMV VLPs could associate strongly with nanocellulose slurry after a simple mixing step.					
31013736	1	2	theme	mosaic	176:181	arg1	particles					194:202	tobacco mosaic virus-like particles	168:202	tobacco mosaic virus-like particles (TMV VLPs)	168:213	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	1	2	theme	mosaic	176:181	arg1	VLPs					209:212	TMV VLPs	205:212	TMV VLPs	205:212	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	1	3	theme	infectious	250:259	arg1	genomes					261:267	infectious genomes	250:267	infectious genomes	250:267	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	3	4	theme	TMV	532:534	arg1	VLPs					536:539	the TMV VLPs	528:539	the TMV VLPs to calcium and phosphate salts	528:570	We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs.					
31013736	1	5	theme	genomes	261:267	arg1	devoid					240:245	devoid	240:245	devoid	240:245	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	1	6	theme	calcium	330:336	arg1	ions					338:341	calcium ions	330:341	calcium ions	330:341	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	4	7	theme	TMV	727:729	arg1	VLPs					731:734	The TMV VLPs	723:734	The TMV VLPs	723:734	The TMV VLPs confer mineralization properties to the nanocellulose for the generation of new composite materials.					
31013736	0	8	theme	Calcium	123:129	arg1	Precursors					131:140	Calcium Precursors	123:140	Calcium Precursors	123:140	Virus-Like Particle Facilitated Deposition of Hydroxyapatite Bone Mineral on Nanocellulose after Exposure to Phosphate and Calcium Precursors.					
31013736	4	9	theme	composite	816:824	arg1	materials					826:834	new composite materials	812:834	new composite materials	812:834	The TMV VLPs confer mineralization properties to the nanocellulose for the generation of new composite materials.					
31013736	4	10	theme	mineralization	743:756	arg1	properties					758:767	mineralization properties	743:767	mineralization properties	743:767	The TMV VLPs confer mineralization properties to the nanocellulose for the generation of new composite materials.					
31013736	1	11	theme	virus-like	183:192	arg1	particles					194:202	tobacco mosaic virus-like particles	168:202	tobacco mosaic virus-like particles (TMV VLPs)	168:213	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	1	11	theme	virus-like	183:192	arg1	VLPs					209:212	TMV VLPs	205:212	TMV VLPs	205:212	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	0	12	theme	Hydroxyapatite	46:59	arg1	Deposition					32:41	Deposition	32:41	Deposition of Hydroxyapatite Bone Mineral on Nanocellulose	32:89	Virus-Like Particle Facilitated Deposition of Hydroxyapatite Bone Mineral on Nanocellulose after Exposure to Phosphate and Calcium Precursors.					
31013736	2	13	theme	nanocellulose	412:424	arg1	slurry					426:431	nanocellulose slurry	412:431	nanocellulose slurry	412:431	Moreover, we showed that the TMV VLPs could associate strongly with nanocellulose slurry after a simple mixing step.					
31013736	0	14	theme	Virus-Like	0:9	arg1	Particle					11:18	Virus-Like Particle	0:18	Virus-Like Particle	0:18	Virus-Like Particle Facilitated Deposition of Hydroxyapatite Bone Mineral on Nanocellulose after Exposure to Phosphate and Calcium Precursors.					
31013736	4	15	theme	materials	826:834	arg1	generation					798:807	the generation	794:807	the generation of new composite materials	794:834	The TMV VLPs confer mineralization properties to the nanocellulose for the generation of new composite materials.					
31013736	3	16	dep	exposed	477:483	arg1	mixed					517:521	mixed	517:521	mixed with the TMV VLPs to calcium and phosphate salts	517:570	We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs.					
31013736	3	16	dep	exposed	477:483	arg1	utilized					576:583	utilized	576:583	utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs	576:720	We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs.					
31013736	1	17	theme	TMV	205:207	arg1	particles					194:202	tobacco mosaic virus-like particles	168:202	tobacco mosaic virus-like particles (TMV VLPs)	168:213	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	1	17	theme	TMV	205:207	arg1	VLPs					209:212	TMV VLPs	205:212	TMV VLPs	205:212	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	3	18	theme	TMV	713:715	arg1	VLPs					717:720	the TMV VLPs	709:720	the TMV VLPs	709:720	We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs.					
31013736	1	19	contain	have	290:293	arg2	charge					310:315	a net negative charge	295:315	a net negative charge	295:315	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	1	19	contain	have	290:293	arg1	they					285:288	they	285:288	they	285:288	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	2	20	theme	simple	441:446	arg1	step					455:458	a simple mixing step	439:458	a simple mixing step	439:458	Moreover, we showed that the TMV VLPs could associate strongly with nanocellulose slurry after a simple mixing step.					
31013736	3	21	theme	phosphate	556:564	arg1	salts					566:570	calcium and phosphate salts	544:570	calcium and phosphate salts	544:570	We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs.					
31013736	2	22	theme	TMV	373:375	arg1	VLPs					377:380	the TMV VLPs	369:380	the TMV VLPs	369:380	Moreover, we showed that the TMV VLPs could associate strongly with nanocellulose slurry after a simple mixing step.					
31013736	0	23	theme	Mineral	66:72	arg1	Hydroxyapatite					46:59	Hydroxyapatite	46:59	Hydroxyapatite Bone Mineral	46:72	Virus-Like Particle Facilitated Deposition of Hydroxyapatite Bone Mineral on Nanocellulose after Exposure to Phosphate and Calcium Precursors.					
31013736	3	24	theme	calcium	544:550	arg1	salts					566:570	calcium and phosphate salts	544:570	calcium and phosphate salts	544:570	We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs.					
31013736	1	25	theme	net	297:299	arg1	charge					310:315	a net negative charge	295:315	a net negative charge	295:315	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	4	26	theme	new	812:814	arg1	materials					826:834	new composite materials	812:834	new composite materials	812:834	The TMV VLPs confer mineralization properties to the nanocellulose for the generation of new composite materials.					
31013736	1	27	theme	negative	301:308	arg1	charge					310:315	a net negative charge	295:315	a net negative charge	295:315	We produced and isolated tobacco mosaic virus-like particles (TMV VLPs) from bacteria, which are devoid of infectious genomes, and found that they have a net negative charge and can bind calcium ions.					
31013736	3	28	theme	physicochemical	585:599	arg1	approaches					601:610	physicochemical approaches	585:610	physicochemical approaches	585:610	We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs.					
31013736	3	29	theme	mineral	637:643	arg1	bone					632:635	bone mineral	632:643	bone mineral (hydroxyapatite)	632:660	We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs.					
31013736	3	29	theme	mineral	637:643	arg1	hydroxyapatite					646:659	hydroxyapatite	646:659	hydroxyapatite	646:659	We sequentially exposed nanocellulose alone or slurries mixed with the TMV VLPs to calcium and phosphate salts and utilized physicochemical approaches to demonstrate that bone mineral (hydroxyapatite) was deposited only in nanocellulose mixed with the TMV VLPs.					
31013736	0	30	from	Deposition	32:41	arg1	Nanocellulose					77:89	Nanocellulose	77:89	Nanocellulose	77:89	Virus-Like Particle Facilitated Deposition of Hydroxyapatite Bone Mineral on Nanocellulose after Exposure to Phosphate and Calcium Precursors.					
30089435	11	0	theme	mucous	1293:1298	arg1	graft					1309:1313	buccal mucous membrane graft	1286:1313	buccal mucous membrane graft	1286:1313	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	8	1	theme	new	1040:1042	arg1	fornix					1044:1049	the new fornix	1036:1049	the new fornix	1036:1049	The line of union between the two grafts formed the apex of the new fornix.					
30089435	1	2	theme	hard	143:146	arg1	graft					176:180	a hard palate-dermis fat composite graft	141:180	a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket	141:243	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	1	2	theme	hard	143:146	arg1	HPDFG					183:187	HPDFG	183:187	HPDFG	183:187	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	1	3	theme	socket	238:243	arg1	reconstruction					194:207	reconstruction	194:207	reconstruction of a contracted anophthalmic socket	194:243	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	0	4	theme	socket	85:90	arg1	reconstruction					43:56	reconstruction	43:56	reconstruction of contracted anophthalmic socket	43:90	Hard palate-dermis fat composite graft for reconstruction of contracted anophthalmic socket.					
30089435	4	5	theme	hard	507:510	arg1	HPG					526:528	HPG	526:528	HPG	526:528	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	4	5	theme	hard	507:510	arg1	graft					519:523	A harvested hard palate graft	495:523	A harvested hard palate graft (HPG)	495:529	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	13	6	theme	posterior	1620:1628	arg1	lamella					1630:1636	the posterior lamella	1616:1636	the posterior lamella	1616:1636	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	7	7	theme	bulbar	874:879	arg1	conjunctiva					881:891	the bulbar conjunctiva	870:891	the bulbar conjunctiva	870:891	The free edge of the DFG was sutured to the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume.					
30089435	2	8	theme	HPDFG	344:348	arg1	placement					350:358	HPDFG placement	344:358	HPDFG placement	344:358	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.					
30089435	4	9	theme	fat	544:546	arg1	DFG					555:557	DFG	555:557	DFG	555:557	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	4	9	theme	fat	544:546	arg1	graft					548:552	a dermis fat graft	535:552	a dermis fat graft (DFG)	535:558	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	5	10	theme	anterior	671:678	arg1	surface					680:686	the anterior surface	667:686	the anterior surface of the anophthalmic socket	667:713	All adhesions and symblephara between the eyelid and the anterior surface of the anophthalmic socket were lysed.					
30089435	4	11	theme	harvested	497:505	arg1	HPG					526:528	HPG	526:528	HPG	526:528	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	4	11	theme	harvested	497:505	arg1	graft					519:523	A harvested hard palate graft	495:523	A harvested hard palate graft (HPG)	495:529	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	7	12	theme	conjunctiva	881:891	arg1	edge					862:865	the cut edge	854:865	the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume	854:973	The free edge of the DFG was sutured to the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume.					
30089435	13	13	theme	effective	1515:1523	arg1	method					1525:1530	an effective method	1512:1530	an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention	1512:1707	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	13	13	theme	effective	1515:1523	arg1	Conclusions					1480:1490	Conclusions	1480:1490	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.	1480:1708	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	13	13	theme	effective	1515:1523	arg1	HPDFG					1503:1507	Composite HPDFG	1493:1507	Composite HPDFG	1493:1507	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	7	14	theme	free	818:821	arg1	edge					823:826	The free edge	814:826	The free edge of the DFG	814:837	The free edge of the DFG was sutured to the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume.					
30089435	12	15	theme	prosthesis	1464:1473	arg1	fit					1475:1477	excellent cosmesis and comfortable prosthesis fit	1429:1477	fit	1475:1477	All four patients achieved excellent cosmesis and comfortable prosthesis fit.					
30089435	11	16	with	revisions	1271:1279	arg1	graft					1361:1365	full-thickness skin graft	1341:1365	full-thickness skin graft	1341:1365	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	11	16	with	revisions	1271:1279	arg1	flap					1376:1379	pedicle flap	1368:1379	pedicle flap	1368:1379	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	11	16	with	revisions	1271:1279	arg1	graft					1309:1313	buccal mucous membrane graft	1286:1313	buccal mucous membrane graft	1286:1313	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	11	16	with	revisions	1271:1279	arg1	graft					1334:1338	amniotic membrane graft	1316:1338	amniotic membrane graft	1316:1338	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	11	16	with	revisions	1271:1279	arg1	fillers					1393:1399	dermal fillers	1386:1399	dermal fillers	1386:1399	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	10	17	theme	5-fluorouracil	1201:1214	arg1	injections					1223:1232	post-operative 5-fluorouracil (5-FU) injections	1186:1232	post-operative 5-fluorouracil (5-FU) injections	1186:1232	Three patients received post-operative 5-fluorouracil (5-FU) injections.					
30089435	12	18	theme	comfortable	1452:1462	arg1	fit					1475:1477	excellent cosmesis and comfortable prosthesis fit	1429:1477	fit	1475:1477	All four patients achieved excellent cosmesis and comfortable prosthesis fit.					
30089435	5	19	dep	adhesions	618:626	arg1	All					614:616	All	614:616	All	614:616	All adhesions and symblephara between the eyelid and the anterior surface of the anophthalmic socket were lysed.					
30089435	7	20	theme	DFG	835:837	arg1	edge					823:826	The free edge	814:826	The free edge of the DFG	814:837	The free edge of the DFG was sutured to the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume.					
30089435	10	21	theme	post-operative	1186:1199	arg1	5-FU					1217:1220	5-FU	1217:1220	5-FU	1217:1220	Three patients received post-operative 5-fluorouracil (5-FU) injections.					
30089435	10	21	theme	post-operative	1186:1199	arg1	5-fluorouracil					1201:1214	post-operative 5-fluorouracil	1186:1214	post-operative 5-fluorouracil (5-FU) injections	1186:1232	Three patients received post-operative 5-fluorouracil (5-FU) injections.					
30089435	1	22	theme	fat	162:164	arg1	graft					176:180	a hard palate-dermis fat composite graft	141:180	a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket	141:243	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	1	22	theme	fat	162:164	arg1	HPDFG					183:187	HPDFG	183:187	HPDFG	183:187	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	6	23	attach	attached	749:756	arg2	component					735:743	The HPG component	727:743	The HPG component	727:743	The HPG component was attached to the tarsal margin to vertically lengthen the eyelid.					
30089435	6	23	attach	attached	749:756	arg1	margin					772:777	the tarsal margin	761:777	the tarsal margin	761:777	The HPG component was attached to the tarsal margin to vertically lengthen the eyelid.					
30089435	6	24	theme	HPG	731:733	arg1	component					735:743	The HPG component	727:743	The HPG component	727:743	The HPG component was attached to the tarsal margin to vertically lengthen the eyelid.					
30089435	7	25	theme	cut	858:860	arg1	edge					862:865	the cut edge	854:865	the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume	854:973	The free edge of the DFG was sutured to the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume.					
30089435	2	26	theme	Retrospective	255:267	arg1	series					306:311	Retrospective, noncomparative, interventional case series	255:311	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.	246:396	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.					
30089435	11	27	theme	full-thickness	1341:1354	arg1	graft					1361:1365	full-thickness skin graft	1341:1365	full-thickness skin graft	1341:1365	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	1	28	theme	composite	166:174	arg1	graft					176:180	a hard palate-dermis fat composite graft	141:180	a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket	141:243	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	1	28	theme	composite	166:174	arg1	HPDFG					183:187	HPDFG	183:187	HPDFG	183:187	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	0	29	theme	palate-dermis	5:17	arg1	graft					33:37	palate-dermis fat composite graft	5:37	palate-dermis fat composite graft for reconstruction of contracted anophthalmic socket	5:90	Hard palate-dermis fat composite graft for reconstruction of contracted anophthalmic socket.					
30089435	8	30	theme	fornix	1044:1049	arg1	apex					1028:1031	the apex	1024:1031	the apex of the new fornix	1024:1049	The line of union between the two grafts formed the apex of the new fornix.					
30089435	8	31	theme	union	988:992	arg1	line					980:983	The line	976:983	The line of union between the two grafts	976:1015	The line of union between the two grafts formed the apex of the new fornix.					
30089435	5	32	theme	anophthalmic	695:706	arg1	socket					708:713	the anophthalmic socket	691:713	the anophthalmic socket	691:713	All adhesions and symblephara between the eyelid and the anterior surface of the anophthalmic socket were lysed.					
30089435	13	33	theme	Composite	1493:1501	arg1	method					1525:1530	an effective method	1512:1530	an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention	1512:1707	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	13	33	theme	Composite	1493:1501	arg1	Conclusions					1480:1490	Conclusions	1480:1490	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.	1480:1708	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	13	33	theme	Composite	1493:1501	arg1	HPDFG					1503:1507	Composite HPDFG	1493:1507	Composite HPDFG	1493:1507	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	11	34	theme	skin	1356:1359	arg1	graft					1361:1365	full-thickness skin graft	1341:1365	full-thickness skin graft	1341:1365	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	13	35	theme	prosthesis	1688:1697	arg1	retention					1699:1707	successful prosthesis retention	1677:1707	successful prosthesis retention	1677:1707	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	14	36	theme	post-operative	1754:1767	arg1	cicatrization					1769:1781	post-operative cicatrization	1754:1781	post-operative cicatrization	1754:1781	Adjunctive use of 5-FU injections can delay post-operative cicatrization.					
30089435	9	37	theme	lower	1107:1111	arg1	fornices					1117:1124	both the upper and lower lid fornices	1088:1124	fornices	1117:1124	Four HPDFG were used to reconstruct both the upper and lower lid fornices in two severely contracted sockets.					
30089435	11	38	theme	amniotic	1316:1323	arg1	graft					1334:1338	amniotic membrane graft	1316:1338	amniotic membrane graft	1316:1338	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	2	39	dep	Methods	246:252	arg1	series					306:311	Retrospective, noncomparative, interventional case series	255:311	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.	246:396	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.					
30089435	0	40	theme	composite	23:31	arg1	graft					33:37	palate-dermis fat composite graft	5:37	palate-dermis fat composite graft for reconstruction of contracted anophthalmic socket	5:90	Hard palate-dermis fat composite graft for reconstruction of contracted anophthalmic socket.					
30089435	9	41	theme	lid	1113:1115	arg1	fornices					1117:1124	both the upper and lower lid fornices	1088:1124	fornices	1117:1124	Four HPDFG were used to reconstruct both the upper and lower lid fornices in two severely contracted sockets.					
30089435	2	42	theme	case	301:304	arg1	series					306:311	Retrospective, noncomparative, interventional case series	255:311	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.	246:396	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.					
30089435	13	43	theme	successful	1677:1686	arg1	retention					1699:1707	successful prosthesis retention	1677:1707	successful prosthesis retention	1677:1707	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	0	44	theme	fat	19:21	arg1	graft					33:37	palate-dermis fat composite graft	5:37	palate-dermis fat composite graft for reconstruction of contracted anophthalmic socket	5:90	Hard palate-dermis fat composite graft for reconstruction of contracted anophthalmic socket.					
30089435	4	45	theme	dermis	537:542	arg1	DFG					555:557	DFG	555:557	DFG	555:557	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	4	45	theme	dermis	537:542	arg1	graft					548:552	a dermis fat graft	535:552	a dermis fat graft (DFG)	535:558	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	1	46	theme	palate-dermis	148:160	arg1	graft					176:180	a hard palate-dermis fat composite graft	141:180	a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket	141:243	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	1	46	theme	palate-dermis	148:160	arg1	HPDFG					183:187	HPDFG	183:187	HPDFG	183:187	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	11	47	theme	pedicle	1368:1374	arg1	flap					1376:1379	pedicle flap	1368:1379	pedicle flap	1368:1379	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	14	48	theme	injections	1733:1742	arg1	use					1721:1723	Adjunctive use	1710:1723	Adjunctive use of 5-FU injections	1710:1742	Adjunctive use of 5-FU injections can delay post-operative cicatrization.					
30089435	9	49	used	used	1068:1071	arg2	HPDFG					1057:1061	Four HPDFG	1052:1061	Four HPDFG	1052:1061	Four HPDFG were used to reconstruct both the upper and lower lid fornices in two severely contracted sockets.					
30089435	3	50	theme	harvested	411:419	arg1	HPDFGs					421:426	Six harvested HPDFGs	407:426	Six harvested HPDFGs	407:426	Results: Six harvested HPDFGs were placed in four patients with contracted anophthalmic sockets.					
30089435	11	51	theme	membrane	1300:1307	arg1	graft					1309:1313	buccal mucous membrane graft	1286:1313	buccal mucous membrane graft	1286:1313	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	14	52	theme	5-FU	1728:1731	arg1	injections					1733:1742	5-FU injections	1728:1742	5-FU injections	1728:1742	Adjunctive use of 5-FU injections can delay post-operative cicatrization.					
30089435	3	53	with	patients	448:455	arg1	sockets					486:492	contracted anophthalmic sockets	462:492	contracted anophthalmic sockets	462:492	Results: Six harvested HPDFGs were placed in four patients with contracted anophthalmic sockets.					
30089435	12	54	theme	excellent	1429:1437	arg1	cosmesis					1439:1446	excellent cosmesis and comfortable prosthesis fit	1429:1477	cosmesis	1439:1446	All four patients achieved excellent cosmesis and comfortable prosthesis fit.					
30089435	9	55	dep	fornices	1117:1124	arg1	both					1088:1091	both	1088:1091	both	1088:1091	Four HPDFG were used to reconstruct both the upper and lower lid fornices in two severely contracted sockets.					
30089435	7	56	theme	surface	944:950	arg1	area					952:955	the anterior conjunctival surface area	918:955	the anterior conjunctival surface area	918:955	The free edge of the DFG was sutured to the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume.					
30089435	11	57	theme	membrane	1325:1332	arg1	graft					1334:1338	amniotic membrane graft	1316:1338	amniotic membrane graft	1316:1338	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	11	58	theme	additional	1260:1269	arg1	revisions					1271:1279	additional revisions	1260:1279	additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers	1260:1399	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	4	59	theme	palate	512:517	arg1	HPG					526:528	HPG	526:528	HPG	526:528	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	4	59	theme	palate	512:517	arg1	graft					519:523	A harvested hard palate graft	495:523	A harvested hard palate graft (HPG)	495:529	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	5	60	theme	socket	708:713	arg1	symblephara					632:642	symblephara	632:642	symblephara	632:642	All adhesions and symblephara between the eyelid and the anterior surface of the anophthalmic socket were lysed.					
30089435	5	60	theme	socket	708:713	arg1	adhesions					618:626	adhesions	618:626	adhesions	618:626	All adhesions and symblephara between the eyelid and the anterior surface of the anophthalmic socket were lysed.					
30089435	5	60	theme	socket	708:713	arg1	surface					680:686	the anterior surface	667:686	the anterior surface of the anophthalmic socket	667:713	All adhesions and symblephara between the eyelid and the anterior surface of the anophthalmic socket were lysed.					
30089435	3	61	theme	anophthalmic	473:484	arg1	sockets					486:492	contracted anophthalmic sockets	462:492	contracted anophthalmic sockets	462:492	Results: Six harvested HPDFGs were placed in four patients with contracted anophthalmic sockets.					
30089435	13	62	theme	contracted	1552:1561	arg1	socket					1576:1581	a contracted anophthalmic socket	1550:1581	a contracted anophthalmic socket	1550:1581	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	9	63	theme	contracted	1142:1151	arg1	sockets					1153:1159	two severely contracted sockets	1129:1159	two severely contracted sockets	1129:1159	Four HPDFG were used to reconstruct both the upper and lower lid fornices in two severely contracted sockets.					
30089435	7	64	theme	anterior	922:929	arg1	area					952:955	the anterior conjunctival surface area	918:955	the anterior conjunctival surface area	918:955	The free edge of the DFG was sutured to the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume.					
30089435	1	65	theme	novel	116:120	arg1	technique					122:130	a novel technique	114:130	a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket	114:243	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	14	66	theme	Adjunctive	1710:1719	arg1	use					1721:1723	Adjunctive use	1710:1723	Adjunctive use of 5-FU injections	1710:1742	Adjunctive use of 5-FU injections can delay post-operative cicatrization.					
30089435	13	67	theme	anophthalmic	1563:1574	arg1	socket					1576:1581	a contracted anophthalmic socket	1550:1581	a contracted anophthalmic socket	1550:1581	Conclusions: Composite HPDFG is an effective method of reconstructing a contracted anophthalmic socket by restoring volume, lengthening the posterior lamella, and expanding the fornix to allow for successful prosthesis retention.					
30089435	9	68	theme	upper	1097:1101	arg1	fornices					1117:1124	both the upper and lower lid fornices	1088:1124	fornices	1117:1124	Four HPDFG were used to reconstruct both the upper and lower lid fornices in two severely contracted sockets.					
30089435	7	69	theme	conjunctival	931:942	arg1	area					952:955	the anterior conjunctival surface area	918:955	the anterior conjunctival surface area	918:955	The free edge of the DFG was sutured to the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume.					
30089435	2	70	theme	patients	321:328	arg1	series					306:311	Retrospective, noncomparative, interventional case series	255:311	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.	246:396	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.					
30089435	7	71	theme	fornix	961:966	arg1	volume					968:973	fornix volume	961:973	fornix volume	961:973	The free edge of the DFG was sutured to the cut edge of the bulbar conjunctiva to simultaneously expand the anterior conjunctival surface area and fornix volume.					
30089435	11	72	theme	buccal	1286:1291	arg1	graft					1309:1313	buccal mucous membrane graft	1286:1313	buccal mucous membrane graft	1286:1313	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	3	73	theme	contracted	462:471	arg1	sockets					486:492	contracted anophthalmic sockets	462:492	contracted anophthalmic sockets	462:492	Results: Six harvested HPDFGs were placed in four patients with contracted anophthalmic sockets.					
30089435	11	74	theme	dermal	1386:1391	arg1	fillers					1393:1399	dermal fillers	1386:1399	dermal fillers	1386:1399	Three patients underwent additional revisions with buccal mucous membrane graft, amniotic membrane graft, full-thickness skin graft, pedicle flap, and dermal fillers.					
30089435	0	75	theme	anophthalmic	72:83	arg1	socket					85:90	contracted anophthalmic socket	61:90	contracted anophthalmic socket	61:90	Hard palate-dermis fat composite graft for reconstruction of contracted anophthalmic socket.					
30089435	6	76	theme	tarsal	765:770	arg1	margin					772:777	the tarsal margin	761:777	the tarsal margin	761:777	The HPG component was attached to the tarsal margin to vertically lengthen the eyelid.					
30089435	4	77	theme	composite	597:605	arg1	graft					607:611	a composite graft	595:611	a composite graft	595:611	A harvested hard palate graft (HPG) and a dermis fat graft (DFG) were adjoined with sutures to form a composite graft.					
30089435	1	78	theme	contracted	214:223	arg1	socket					238:243	a contracted anophthalmic socket	212:243	a contracted anophthalmic socket	212:243	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
30089435	0	79	theme	contracted	61:70	arg1	socket					85:90	contracted anophthalmic socket	61:90	contracted anophthalmic socket	61:90	Hard palate-dermis fat composite graft for reconstruction of contracted anophthalmic socket.					
30089435	2	80	dep	Retrospective	255:267	arg1	noncomparative					270:283	noncomparative	270:283	noncomparative	270:283	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.					
30089435	2	80	dep	Retrospective	255:267	arg1	interventional					286:299	interventional	286:299	interventional	286:299	Methods: Retrospective, noncomparative, interventional case series of four patients who underwent HPDFG placement by one surgeon between 2010 and 2017.					
30089435	9	81	from	fornices	1117:1124	arg1	sockets					1153:1159	two severely contracted sockets	1129:1159	two severely contracted sockets	1129:1159	Four HPDFG were used to reconstruct both the upper and lower lid fornices in two severely contracted sockets.					
30089435	1	82	theme	anophthalmic	225:236	arg1	socket					238:243	a contracted anophthalmic socket	212:243	a contracted anophthalmic socket	212:243	Purpose: To describe a novel technique of using a hard palate-dermis fat composite graft (HPDFG) for reconstruction of a contracted anophthalmic socket.					
29293865	0	0	theme	walls	77:81	arg1	nature					49:54	the dynamic nature	37:54	the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium	37:114	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	2	1	located	found	372:376	arg1	organs					389:394	growing organs	381:394	growing organs of angiosperms	381:409	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	2	1	located	found	372:376	arg2	tissue					355:360	a strengthening tissue	339:360	a strengthening tissue typically found in growing organs of angiosperms	339:409	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	2	1	located	found	372:376	arg2	collenchyma					324:334	collenchyma	324:334	collenchyma	324:334	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	1	2	theme	key	139:141	arg1	adaptation					154:163	A key structural adaptation	137:163	Aims A key structural adaptation of vascular plants	132:182	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	1	3	theme	tissues	241:247	arg1	Background					117:126	Background	117:126	Background	117:126	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	1	3	theme	tissues	241:247	arg1	evolution					192:200	the evolution	188:200	the evolution of specialized vascular and mechanical tissues	188:247	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	1	3	theme	tissues	241:247	arg1	Aims					132:135	Aims	132:135	Aims A key structural adaptation of vascular plants	132:182	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	0	4	theme	cell	72:75	arg1	walls					77:81	sclerenchyma cell walls	59:81	sclerenchyma cell walls of the fern Asplenium rutifolium	59:114	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	2	5	theme	rutifolium	473:482	arg1	petiole					443:449	the petiole	439:449	the petiole of the fern Asplenium rutifolium	439:482	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	1	6	theme	structural	143:152	arg1	adaptation					154:163	A key structural adaptation	137:163	Aims A key structural adaptation of vascular plants	132:182	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	3	7	theme	ultra	516:520	arg1	Methods					485:491	Methods The in situ cell wall (ultra)structure and composition	485:546	Methods The in situ cell wall (ultra)structure and composition of this tissue	485:561	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	7	theme	ultra	516:520	arg1	structure					522:530	The in situ cell wall (ultra)structure	493:530	The in situ cell wall (ultra)structure	493:530	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	6	8	theme	collenchyma	1186:1196	arg1	properties					1172:1181	the properties	1168:1181	the properties of collenchyma	1168:1196	Conclusions If the term collenchyma is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.					
29293865	6	9	contain	has	1202:1204	arg1	sclerenchyma					1143:1154	sclerenchyma	1143:1154	sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification	1143:1275	Conclusions If the term collenchyma is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.					
29293865	6	9	contain	has	1202:1204	arg2	ability					1210:1216	the ability to modify its mechanical properties through sclerification	1206:1275	the ability to modify its mechanical properties through sclerification	1206:1275	Conclusions If the term collenchyma is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.					
29293865	0	10	theme	Asplenium	95:103	arg1	rutifolium					105:114	the fern Asplenium rutifolium	86:114	the fern Asplenium rutifolium	86:114	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	4	11	theme	angiosperm	877:886	arg1	collenchyma					888:898	angiosperm collenchyma	877:898	angiosperm collenchyma	877:898	Key Results Structurally the mechanical tissue resembles sclerenchyma, while its biomechanical properties and molecular composition both share more characteristics with angiosperm collenchyma.					
29293865	3	12	theme	wall	510:513	arg1	Methods					485:491	Methods The in situ cell wall (ultra)structure and composition	485:546	Methods The in situ cell wall (ultra)structure and composition of this tissue	485:561	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	12	theme	wall	510:513	arg1	structure					522:530	The in situ cell wall (ultra)structure	493:530	The in situ cell wall (ultra)structure	493:530	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	2	13	theme	growing	381:387	arg1	organs					389:394	growing organs	381:394	growing organs of angiosperms	381:409	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	5	14	theme	Cell	901:904	arg1	thickening					911:920	Cell wall thickening	901:920	Cell wall thickening	901:920	Cell wall thickening only occurs late during cell expansion or after cell expansion has ceased.					
29293865	0	15	theme	fern	90:93	arg1	rutifolium					105:114	the fern Asplenium rutifolium	86:114	the fern Asplenium rutifolium	86:114	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	5	16	theme	cell	946:949	arg1	expansion					951:959	cell expansion	946:959	cell expansion	946:959	Cell wall thickening only occurs late during cell expansion or after cell expansion has ceased.					
29293865	3	17	dep	Methods	485:491	arg1	Methods					485:491	Methods The in situ cell wall (ultra)structure and composition	485:546	Methods The in situ cell wall (ultra)structure and composition of this tissue	485:561	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	17	dep	Methods	485:491	arg1	composition					536:546	composition	536:546	composition	536:546	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	17	dep	Methods	485:491	arg1	structure					522:530	The in situ cell wall (ultra)structure	493:530	The in situ cell wall (ultra)structure	493:530	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	5	18	theme	wall	906:909	arg1	thickening					911:920	Cell wall thickening	901:920	Cell wall thickening	901:920	Cell wall thickening only occurs late during cell expansion or after cell expansion has ceased.					
29293865	0	19	theme	in	12:13	arg1	analysis					20:27	Comparative in situ analysis	0:27	Comparative in situ analysis	0:27	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	2	20	theme	Asplenium	463:471	arg1	rutifolium					473:482	the fern Asplenium rutifolium	454:482	the fern Asplenium rutifolium	454:482	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	1	21	theme	vascular	168:175	arg1	plants					177:182	vascular plants	168:182	vascular plants	168:182	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	0	22	theme	Comparative	0:10	arg1	analysis					20:27	Comparative in situ analysis	0:27	Comparative in situ analysis	0:27	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	6	23	theme	term	1016:1019	arg1	collenchyma					1021:1031	the term collenchyma	1012:1031	the term collenchyma	1012:1031	Conclusions If the term collenchyma is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.					
29293865	3	24	dep	in	497:498	arg1	situ					500:503	situ	500:503	situ	500:503	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	1	25	theme	plants	177:182	arg1	adaptation					154:163	A key structural adaptation	137:163	Aims A key structural adaptation of vascular plants	132:182	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	6	26	dep	Conclusions	997:1007	arg1	reserved					1036:1043	reserved	1036:1043	is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.	1033:1276	Conclusions If the term collenchyma is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.					
29293865	4	27	theme	more	851:854	arg1	characteristics					856:870	more characteristics	851:870	more characteristics	851:870	Key Results Structurally the mechanical tissue resembles sclerenchyma, while its biomechanical properties and molecular composition both share more characteristics with angiosperm collenchyma.					
29293865	1	28	dep	Aims	132:135	arg1	adaptation					154:163	A key structural adaptation	137:163	Aims A key structural adaptation of vascular plants	132:182	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	6	29	from	tissue	1108:1113	arg1	rutifolium					1121:1130	A. rutifolium	1118:1130	A. rutifolium	1118:1130	Conclusions If the term collenchyma is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.					
29293865	2	30	theme	similar	414:420	arg1	tissue					422:427	a similar tissue	412:427	a similar tissue	412:427	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	4	31	theme	mechanical	737:746	arg1	tissue					748:753	the mechanical tissue	733:753	the mechanical tissue	733:753	Key Results Structurally the mechanical tissue resembles sclerenchyma, while its biomechanical properties and molecular composition both share more characteristics with angiosperm collenchyma.					
29293865	0	32	theme	rutifolium	105:114	arg1	walls					77:81	sclerenchyma cell walls	59:81	sclerenchyma cell walls of the fern Asplenium rutifolium	59:114	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	2	33	theme	strengthening	341:353	arg1	tissue					355:360	a strengthening tissue	339:360	a strengthening tissue typically found in growing organs of angiosperms	339:409	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	2	33	theme	strengthening	341:353	arg1	collenchyma					324:334	collenchyma	324:334	collenchyma	324:334	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	2	34	theme	fern	458:461	arg1	rutifolium					473:482	the fern Asplenium rutifolium	454:482	the fern Asplenium rutifolium	454:482	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	1	35	theme	novel	287:291	arg1	architectures					303:315	novel cell wall architectures	287:315	novel cell wall architectures	287:315	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	4	36	dep	properties	803:812	arg1	both					840:843	both	840:843	both	840:843	Key Results Structurally the mechanical tissue resembles sclerenchyma, while its biomechanical properties and molecular composition both share more characteristics with angiosperm collenchyma.					
29293865	1	37	theme	cell	293:296	arg1	architectures					303:315	novel cell wall architectures	287:315	novel cell wall architectures	287:315	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	2	38	theme	angiosperms	399:409	arg1	organs					389:394	growing organs	381:394	growing organs of angiosperms	381:409	While collenchyma is a strengthening tissue typically found in growing organs of angiosperms, a similar tissue occurs in the petiole of the fern Asplenium rutifolium.					
29293865	1	39	theme	wall	298:301	arg1	architectures					303:315	novel cell wall architectures	287:315	novel cell wall architectures	287:315	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	0	40	theme	dynamic	41:47	arg1	nature					49:54	the dynamic nature	37:54	the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium	37:114	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	3	41	theme	wide-angle	664:673	arg1	diffraction					681:691	wide-angle X-ray diffraction	664:691	wide-angle X-ray diffraction	664:691	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	42	theme	tissue	556:561	arg1	Methods					485:491	Methods The in situ cell wall (ultra)structure and composition	485:546	Methods The in situ cell wall (ultra)structure and composition of this tissue	485:561	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	42	theme	tissue	556:561	arg1	composition					536:546	composition	536:546	composition	536:546	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	42	theme	tissue	556:561	arg1	structure					522:530	The in situ cell wall (ultra)structure	493:530	The in situ cell wall (ultra)structure	493:530	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	43	theme	in	497:498	arg1	Methods					485:491	Methods The in situ cell wall (ultra)structure and composition	485:546	Methods The in situ cell wall (ultra)structure and composition of this tissue	485:561	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	43	theme	in	497:498	arg1	structure					522:530	The in situ cell wall (ultra)structure	493:530	The in situ cell wall (ultra)structure	493:530	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	4	44	theme	Key	708:710	arg1	Results					712:718	Key Results	708:718	Key Results	708:718	Key Results Structurally the mechanical tissue resembles sclerenchyma, while its biomechanical properties and molecular composition both share more characteristics with angiosperm collenchyma.					
29293865	6	45	theme	mechanical	1232:1241	arg1	properties					1243:1252	its mechanical properties	1228:1252	its mechanical properties	1228:1252	Conclusions If the term collenchyma is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.					
29293865	3	46	theme	X-ray	675:679	arg1	diffraction					681:691	wide-angle X-ray diffraction	664:691	wide-angle X-ray diffraction	664:691	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	1	47	theme	specialized	205:215	arg1	tissues					241:247	specialized vascular and mechanical tissues	205:247	specialized vascular and mechanical tissues	205:247	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	4	48	theme	biomechanical	789:801	arg1	properties					803:812	its biomechanical properties	785:812	its biomechanical properties	785:812	Key Results Structurally the mechanical tissue resembles sclerenchyma, while its biomechanical properties and molecular composition both share more characteristics with angiosperm collenchyma.					
29293865	6	49	theme	mechanical	1097:1106	arg1	tissue					1108:1113	the mechanical tissue	1093:1113	the mechanical tissue in A. rutifolium	1093:1130	Conclusions If the term collenchyma is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.					
29293865	5	50	theme	cell	970:973	arg1	expansion					975:983	cell expansion	970:983	cell expansion	970:983	Cell wall thickening only occurs late during cell expansion or after cell expansion has ceased.					
29293865	1	51	theme	vascular	217:224	arg1	tissues					241:247	specialized vascular and mechanical tissues	205:247	specialized vascular and mechanical tissues	205:247	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
29293865	0	52	theme	sclerenchyma	59:70	arg1	walls					77:81	sclerenchyma cell walls	59:81	sclerenchyma cell walls of the fern Asplenium rutifolium	59:114	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	6	53	theme	expansive	1075:1083	arg1	growth					1085:1090	expansive growth	1075:1090	expansive growth	1075:1090	Conclusions If the term collenchyma is reserved for walls that thicken during expansive growth, the mechanical tissue in A. rutifolium represents sclerenchyma that mimics the properties of collenchyma and has the ability to modify its mechanical properties through sclerification.					
29293865	0	54	dep	in	12:13	arg1	situ					15:18	situ	15:18	situ	15:18	Comparative in situ analysis reveals the dynamic nature of sclerenchyma cell walls of the fern Asplenium rutifolium.					
29293865	4	55	theme	molecular	818:826	arg1	composition					828:838	molecular composition	818:838	molecular composition	818:838	Key Results Structurally the mechanical tissue resembles sclerenchyma, while its biomechanical properties and molecular composition both share more characteristics with angiosperm collenchyma.					
29293865	3	56	theme	cell	505:508	arg1	Methods					485:491	Methods The in situ cell wall (ultra)structure and composition	485:546	Methods The in situ cell wall (ultra)structure and composition of this tissue	485:561	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	3	56	theme	cell	505:508	arg1	structure					522:530	The in situ cell wall (ultra)structure	493:530	The in situ cell wall (ultra)structure	493:530	Methods The in situ cell wall (ultra)structure and composition of this tissue was investigated and characterized mechanically as well as structurally through nano-indentation and wide-angle X-ray diffraction, respectively.					
29293865	1	57	theme	mechanical	230:239	arg1	tissues					241:247	specialized vascular and mechanical tissues	205:247	specialized vascular and mechanical tissues	205:247	Background and Aims A key structural adaptation of vascular plants was the evolution of specialized vascular and mechanical tissues, innovations likely to have generated novel cell wall architectures.					
30913433	1	0	theme	stirred-tank	331:342	arg1	reactors					344:351	continuous stirred-tank reactors	320:351	continuous stirred-tank reactors (CSTR)	320:358	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	0	theme	stirred-tank	331:342	arg1	CSTR					354:357	CSTR	354:357	CSTR	354:357	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	6	1	theme	hydrogen	1027:1034	arg1	production					1036:1045	hydrogen production	1027:1045	hydrogen production from binary hydrolysates of agave bagasse	1027:1087	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	6	1	theme	hydrogen	1027:1034	arg1	alternative					1126:1136	the most promising alternative	1107:1136	the most promising alternative evaluated with scale-up potential for the production of energy biofuels	1107:1208	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	4	2	theme	VHPR	784:787	arg1	differences					769:779	differences	769:779	differences of VHPR between hydrolysates	769:808	Differences on VHPR between reactors could be explained by substrate availability, which is intrinsic to the growth mode of each reactor configuration; while differences of VHPR between hydrolysates were possibly related to the composition of enzymatic hydrolysates.					
30913433	1	3	theme	H2	178:179	arg1	production					182:191	Continuous hydrogen (H2) production	157:191	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse	157:301	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	4	4	from	Differences	611:621	arg1	VHPR					626:629	VHPR	626:629	VHPR	626:629	Differences on VHPR between reactors could be explained by substrate availability, which is intrinsic to the growth mode of each reactor configuration; while differences of VHPR between hydrolysates were possibly related to the composition of enzymatic hydrolysates.					
30913433	0	5	theme	bagasse	106:112	arg1	hydrolysates					84:95	individual and binary enzymatic hydrolysates	52:95	individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors	52:154	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	2	6	from	rates	423:427	arg1	CSTR					439:442	CSTR	439:442	CSTR	439:442	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	6	7	theme	bagasse	1081:1087	arg1	hydrolysates					1059:1070	binary hydrolysates	1052:1070	binary hydrolysates of agave bagasse	1052:1087	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	0	8	theme	agave	100:104	arg1	bagasse					106:112	agave bagasse	100:112	agave bagasse	100:112	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	6	9	theme	promising	1116:1124	arg1	production					1036:1045	hydrogen production	1027:1045	hydrogen production from binary hydrolysates of agave bagasse	1027:1087	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	6	9	theme	promising	1116:1124	arg1	alternative					1126:1136	the most promising alternative	1107:1136	the most promising alternative evaluated with scale-up potential for the production of energy biofuels	1107:1208	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	2	10	theme	production	412:421	arg1	rates					423:427	The volumetric H2 production rates	394:427	The volumetric H2 production rates (VHPR) in CSTR	394:442	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	2	10	theme	production	412:421	arg1	H2/L-d					463:468	13 and 2.25 L H2/L-d	449:468	13 and 2.25 L H2/L-d	449:468	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	2	10	theme	production	412:421	arg1	VHPR					430:433	VHPR	430:433	VHPR	430:433	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	0	11	theme	suspended-culture	117:133	arg1	reactors					147:154	suspended-culture and biofilm reactors	117:154	suspended-culture and biofilm reactors	117:154	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	6	12	theme	hydrolysis	1015:1024	arg1	yields					994:999	H2 yields	991:999	H2 yields	991:999	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	6	12	theme	hydrolysis	1015:1024	arg1	costs					1006:1010	costs	1006:1010	costs of hydrolysis	1006:1024	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	6	12	theme	hydrolysis	1015:1024	arg1	VHPR					985:988	VHPR	985:988	VHPR	985:988	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	6	13	theme	energy	1194:1199	arg1	biofuels					1201:1208	energy biofuels	1194:1208	energy biofuels	1194:1208	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	1	14	theme	trickling	364:372	arg1	TBR					388:390	TBR	388:390	TBR	388:390	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	14	theme	trickling	364:372	arg1	reactors					378:385	trickling bed reactors	364:385	trickling bed reactors (TBR)	364:391	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	6	15	theme	biofuels	1201:1208	arg1	production					1180:1189	the production	1176:1189	the production of energy biofuels	1176:1208	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	2	16	with	H2/L-d	463:468	arg1	IH					482:483	IH	482:483	IH	482:483	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	2	16	with	H2/L-d	463:468	arg1	BH					475:476	BH	475:476	BH	475:476	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	1	17	theme	enzymatic	263:271	arg1	hydrolysates					273:284	individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates	198:284	individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse	198:301	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	18	from	hydrolysates	273:284	arg1	production					182:191	Continuous hydrogen (H2) production	157:191	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse	157:301	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	19	theme	individual	198:207	arg1	hydrolysates					273:284	individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates	198:284	individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse	198:301	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	0	20	theme	hydrogen	26:33	arg1	production					35:44	continuous hydrogen production	15:44	continuous hydrogen production	15:44	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	0	21	theme	biofilm	139:145	arg1	reactors					147:154	suspended-culture and biofilm reactors	117:154	suspended-culture and biofilm reactors	117:154	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	2	22	theme	volumetric	398:407	arg1	rates					423:427	The volumetric H2 production rates	394:427	The volumetric H2 production rates (VHPR) in CSTR	394:442	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	2	22	theme	volumetric	398:407	arg1	H2/L-d					463:468	13 and 2.25 L H2/L-d	449:468	13 and 2.25 L H2/L-d	449:468	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	2	22	theme	volumetric	398:407	arg1	VHPR					430:433	VHPR	430:433	VHPR	430:433	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	0	23	theme	continuous	15:24	arg1	production					35:44	continuous hydrogen production	15:44	continuous hydrogen production	15:44	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	1	24	theme	bed	374:376	arg1	TBR					388:390	TBR	388:390	TBR	388:390	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	24	theme	bed	374:376	arg1	reactors					378:385	trickling bed reactors	364:385	trickling bed reactors (TBR)	364:391	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	4	25	theme	substrate	670:678	arg1	availability					680:691	substrate availability	670:691	substrate availability	670:691	Differences on VHPR between reactors could be explained by substrate availability, which is intrinsic to the growth mode of each reactor configuration; while differences of VHPR between hydrolysates were possibly related to the composition of enzymatic hydrolysates.					
30913433	1	26	theme	agave	289:293	arg1	bagasse					295:301	agave bagasse	289:301	agave bagasse	289:301	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	4	27	theme	hydrolysates	864:875	arg1	composition					839:849	the composition	835:849	the composition of enzymatic hydrolysates	835:875	Differences on VHPR between reactors could be explained by substrate availability, which is intrinsic to the growth mode of each reactor configuration; while differences of VHPR between hydrolysates were possibly related to the composition of enzymatic hydrolysates.					
30913433	0	28	from	hydrolysates	84:95	arg1	reactors					147:154	suspended-culture and biofilm reactors	117:154	suspended-culture and biofilm reactors	117:154	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	5	29	theme	transfer	952:959	arg1	conditions					961:970	substrate transfer conditions	942:970	substrate transfer conditions	942:970	Furthermore, homoacetogenesis was strongly influenced by H2 and substrate transfer conditions.					
30913433	6	30	theme	H2	991:992	arg1	yields					994:999	H2 yields	991:999	H2 yields	991:999	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	0	31	theme	production	35:44	arg1	Improvement					0:10	Improvement	0:10	Improvement of continuous hydrogen production	0:44	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	1	32	theme	bagasse	295:301	arg1	hydrolysates					273:284	individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates	198:284	individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse	198:301	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	4	33	theme	enzymatic	854:862	arg1	hydrolysates					864:875	enzymatic hydrolysates	854:875	enzymatic hydrolysates	854:875	Differences on VHPR between reactors could be explained by substrate availability, which is intrinsic to the growth mode of each reactor configuration; while differences of VHPR between hydrolysates were possibly related to the composition of enzymatic hydrolysates.					
30913433	3	34	theme	TBR	562:564	arg1	configuration					566:578	the TBR configuration	558:578	the TBR configuration	558:578	Meanwhile, VHPR of 5.76 and 2.0 L H2/L-d were obtained in the TBR configuration using BH and IH, respectively.					
30913433	1	35	dep	individual	198:207	arg1	Stonezyme					210:218	Stonezyme	210:218	Stonezyme	210:218	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	35	dep	individual	198:207	arg1	BH					259:260	BH	259:260	BH	259:260	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	35	dep	individual	198:207	arg1	Celluclast-Viscozyme					237:256	Celluclast-Viscozyme	237:256	Celluclast-Viscozyme	237:256	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	6	36	from	hydrolysates	1059:1070	arg1	production					1036:1045	hydrogen production	1027:1045	hydrogen production from binary hydrolysates of agave bagasse	1027:1087	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	6	36	from	hydrolysates	1059:1070	arg1	alternative					1126:1136	the most promising alternative	1107:1136	the most promising alternative evaluated with scale-up potential for the production of energy biofuels	1107:1208	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	4	37	theme	configuration	748:760	arg1	mode					727:730	the growth mode	716:730	the growth mode of each reactor configuration	716:760	Differences on VHPR between reactors could be explained by substrate availability, which is intrinsic to the growth mode of each reactor configuration; while differences of VHPR between hydrolysates were possibly related to the composition of enzymatic hydrolysates.					
30913433	6	38	theme	agave	1075:1079	arg1	bagasse					1081:1087	agave bagasse	1075:1087	agave bagasse	1075:1087	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	0	39	theme	individual	52:61	arg1	hydrolysates					84:95	individual and binary enzymatic hydrolysates	52:95	individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors	52:154	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	4	40	theme	reactor	740:746	arg1	configuration					748:760	each reactor configuration	735:760	each reactor configuration	735:760	Differences on VHPR between reactors could be explained by substrate availability, which is intrinsic to the growth mode of each reactor configuration; while differences of VHPR between hydrolysates were possibly related to the composition of enzymatic hydrolysates.					
30913433	4	41	theme	growth	720:725	arg1	mode					727:730	the growth mode	716:730	the growth mode of each reactor configuration	716:760	Differences on VHPR between reactors could be explained by substrate availability, which is intrinsic to the growth mode of each reactor configuration; while differences of VHPR between hydrolysates were possibly related to the composition of enzymatic hydrolysates.					
30913433	3	42	theme	H2/L-d	534:539	arg1	VHPR					511:514	VHPR	511:514	VHPR of 5.76 and 2.0 L H2/L-d	511:539	Meanwhile, VHPR of 5.76 and 2.0 L H2/L-d were obtained in the TBR configuration using BH and IH, respectively.					
30913433	3	42	theme	H2/L-d	534:539	arg1	Meanwhile					500:508	Meanwhile	500:508	Meanwhile	500:508	Meanwhile, VHPR of 5.76 and 2.0 L H2/L-d were obtained in the TBR configuration using BH and IH, respectively.					
30913433	6	43	theme	scale-up	1153:1160	arg1	potential					1162:1170	scale-up potential	1153:1170	scale-up potential	1153:1170	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	6	44	theme	binary	1052:1057	arg1	hydrolysates					1059:1070	binary hydrolysates	1052:1070	binary hydrolysates of agave bagasse	1052:1087	Considering VHPR, H2 yields, and costs of hydrolysis, hydrogen production from binary hydrolysates of agave bagasse was identified as the most promising alternative evaluated with scale-up potential for the production of energy biofuels.					
30913433	0	45	theme	enzymatic	74:82	arg1	hydrolysates					84:95	individual and binary enzymatic hydrolysates	52:95	individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors	52:154	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	5	46	theme	substrate	942:950	arg1	conditions					961:970	substrate transfer conditions	942:970	substrate transfer conditions	942:970	Furthermore, homoacetogenesis was strongly influenced by H2 and substrate transfer conditions.					
30913433	2	47	theme	H2	409:410	arg1	rates					423:427	The volumetric H2 production rates	394:427	The volumetric H2 production rates (VHPR) in CSTR	394:442	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	2	47	theme	H2	409:410	arg1	H2/L-d					463:468	13 and 2.25 L H2/L-d	449:468	13 and 2.25 L H2/L-d	449:468	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	2	47	theme	H2	409:410	arg1	VHPR					430:433	VHPR	430:433	VHPR	430:433	The volumetric H2 production rates (VHPR) in CSTR were 13 and 2.25 L H2/L-d with BH and IH, respectively.					
30913433	1	48	theme	Continuous	157:166	arg1	production					182:191	Continuous hydrogen (H2) production	157:191	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse	157:301	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	49	theme	binary	229:234	arg1	hydrolysates					273:284	individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates	198:284	individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse	198:301	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	0	50	theme	binary	67:72	arg1	hydrolysates					84:95	individual and binary enzymatic hydrolysates	52:95	individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors	52:154	Improvement of continuous hydrogen production using individual and binary enzymatic hydrolysates of agave bagasse in suspended-culture and biofilm reactors.					
30913433	1	51	theme	continuous	320:329	arg1	reactors					344:351	continuous stirred-tank reactors	320:351	continuous stirred-tank reactors (CSTR)	320:358	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	51	theme	continuous	320:329	arg1	CSTR					354:357	CSTR	354:357	CSTR	354:357	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	52	dep	Stonezyme	210:218	arg1	IH					221:222	IH	221:222	IH	221:222	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
30913433	1	53	theme	hydrogen	168:175	arg1	production					182:191	Continuous hydrogen (H2) production	157:191	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse	157:301	Continuous hydrogen (H2) production from individual (Stonezyme, IH) and binary (Celluclast-Viscozyme, BH) enzymatic hydrolysates of agave bagasse was evaluated in continuous stirred-tank reactors (CSTR) and trickling bed reactors (TBR).					
29653214	5	0	theme	organic	1119:1125	arg1	buffer					1127:1132	organic buffer	1119:1132	organic buffer	1119:1132	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	0	1	theme	pH-	66:68	arg1	manner					89:94	a pH- and salt-dependent manner	64:94	a pH- and salt-dependent manner	64:94	Poly(propyleneimine) glycodendrimers non-covalently bind ATP in a pH- and salt-dependent manner - model studies for adenosine analogue drug delivery.					
29653214	4	2	from	groups	943:948	arg1	nucleotide					957:966	the nucleotide	953:966	the nucleotide	953:966	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	4	2	from	groups	943:948	arg1	dendrimer					976:984	the dendrimer	972:984	the dendrimer	972:984	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	4	3	theme	amino	937:941	arg1	groups					943:948	the ionized phosphate and amino groups	911:948	groups	943:948	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	2	4	theme	direct	364:369	arg1	delivery					371:378	direct delivery	364:378	direct delivery of active triphosphate forms	364:407	Therefore, application of nanocarrier systems for direct delivery of active triphosphate forms has been proposed.					
29653214	5	5	dep	pH	1139:1140	arg1	sites					1156:1160	more binding sites	1143:1160	more binding sites in acidic pH	1143:1173	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	5	6	theme	buffer	1073:1078	arg1	composition					1080:1090	buffer composition	1073:1090	buffer composition (strongest interactions in organic buffer)	1073:1133	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	4	7	theme	nucleoside	818:827	arg1	triphosphates					829:841	nucleoside triphosphates	818:841	nucleoside triphosphates	818:841	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	3	8	theme	poly	606:609	arg1	dendrimers					627:636	4th generation poly(propyleneimine) dendrimers	591:636	4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility)	591:698	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	0	9	theme	salt-dependent	74:87	arg1	manner					89:94	a pH- and salt-dependent manner	64:94	a pH- and salt-dependent manner	64:94	Poly(propyleneimine) glycodendrimers non-covalently bind ATP in a pH- and salt-dependent manner - model studies for adenosine analogue drug delivery.					
29653214	4	10	contain	have	772:775	arg1	glycodendrimers					756:770	glycodendrimers	756:770	glycodendrimers	756:770	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	4	10	contain	have	772:775	arg2	ability					781:787	the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively	777:998	the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively	777:998	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	6	11	theme	adenosine	1280:1288	arg1	analogues					1290:1298	adenosine analogues	1280:1298	adenosine analogues	1280:1298	These properties allow us to consider maltose-modified dendrimers as especially promising carriers for adenosine analogues.					
29653214	3	12	theme	4th	591:593	arg1	dendrimers					627:636	4th generation poly(propyleneimine) dendrimers	591:636	4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility)	591:698	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	1	13	theme	subsequent	262:271	arg1	phosphorylation					273:287	subsequent phosphorylation	262:287	subsequent phosphorylation for anticancer activity	262:311	Adenosine analogue drugs (such as fludarabine or cladribine) require transporter-mediated uptake into cells and subsequent phosphorylation for anticancer activity.					
29653214	0	14	theme	model	98:102	arg1	studies					104:110	model studies	98:110	model studies for adenosine analogue drug delivery	98:147	Poly(propyleneimine) glycodendrimers non-covalently bind ATP in a pH- and salt-dependent manner - model studies for adenosine analogue drug delivery.					
29653214	4	15	from	phosphate	923:931	arg1	nucleotide					957:966	the nucleotide	953:966	the nucleotide	953:966	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	4	15	from	phosphate	923:931	arg1	dendrimer					976:984	the dendrimer	972:984	the dendrimer	972:984	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	4	16	theme	ionized	915:921	arg1	phosphate					923:931	the ionized phosphate and amino groups	911:948	phosphate	923:931	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	3	17	theme	potential	487:495	arg1	titration					497:505	zeta potential titration	482:505	zeta potential titration	482:505	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	1	18	theme	analogue	160:167	arg1	drugs					169:173	Adenosine analogue drugs	150:173	Adenosine analogue drugs (such as fludarabine or cladribine)	150:209	Adenosine analogue drugs (such as fludarabine or cladribine) require transporter-mediated uptake into cells and subsequent phosphorylation for anticancer activity.					
29653214	3	19	theme	interactions	570:581	arg1	nucleotide					729:738	a model adenosine nucleotide	711:738	a model adenosine nucleotide	711:738	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	19	theme	interactions	570:581	arg1	stoichiometry					524:536	the stoichiometry	520:536	the stoichiometry	520:536	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	19	theme	interactions	570:581	arg1	parameters					556:565	thermodynamic parameters	542:565	thermodynamic parameters	542:565	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	20	theme	titration	456:464	arg1	calorimetry					466:476	isothermal titration calorimetry	445:476	isothermal titration calorimetry	445:476	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	6	21	theme	maltose-modified	1215:1230	arg1	carriers					1267:1274	especially promising carriers	1246:1274	especially promising carriers for adenosine analogues	1246:1298	These properties allow us to consider maltose-modified dendrimers as especially promising carriers for adenosine analogues.					
29653214	6	21	theme	maltose-modified	1215:1230	arg1	dendrimers					1232:1241	maltose-modified dendrimers	1215:1241	maltose-modified dendrimers	1215:1241	These properties allow us to consider maltose-modified dendrimers as especially promising carriers for adenosine analogues.					
29653214	5	22	theme	complexation	1005:1016	arg1	enthalpy-driven					1042:1056	enthalpy-driven	1042:1056	enthalpy-driven	1042:1056	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	5	22	theme	complexation	1005:1016	arg1	spontaneous					1029:1039	spontaneous	1029:1039	spontaneous	1029:1039	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	5	22	theme	complexation	1005:1016	arg1	process					1018:1024	The complexation process	1001:1024	The complexation process	1001:1024	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	5	23	theme	acidic	1165:1170	arg1	pH					1172:1173	acidic pH	1165:1173	acidic pH	1165:1173	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	2	24	theme	forms	403:407	arg1	delivery					371:378	direct delivery	364:378	direct delivery of active triphosphate forms	364:407	Therefore, application of nanocarrier systems for direct delivery of active triphosphate forms has been proposed.					
29653214	3	25	theme	propyleneimine	611:624	arg1	dendrimers					627:636	4th generation poly(propyleneimine) dendrimers	591:636	4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility)	591:698	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	2	26	theme	triphosphate	390:401	arg1	forms					403:407	active triphosphate forms	383:407	active triphosphate forms	383:407	Therefore, application of nanocarrier systems for direct delivery of active triphosphate forms has been proposed.					
29653214	0	27	dep	glycodendrimers	21:35	arg1	studies					104:110	model studies	98:110	model studies for adenosine analogue drug delivery	98:147	Poly(propyleneimine) glycodendrimers non-covalently bind ATP in a pH- and salt-dependent manner - model studies for adenosine analogue drug delivery.					
29653214	0	27	dep	glycodendrimers	21:35	arg1	bind					52:55	bind	52:55	glycodendrimers non-covalently bind ATP in a pH- and salt-dependent manner	21:94	Poly(propyleneimine) glycodendrimers non-covalently bind ATP in a pH- and salt-dependent manner - model studies for adenosine analogue drug delivery.					
29653214	3	28	theme	generation	595:604	arg1	dendrimers					627:636	4th generation poly(propyleneimine) dendrimers	591:636	4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility)	591:698	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	5	29	theme	strongest	1093:1101	arg1	interactions					1103:1114	strongest interactions	1093:1114	strongest interactions in organic buffer	1093:1132	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	3	30	theme	zeta	482:485	arg1	titration					497:505	zeta potential titration	482:505	zeta potential titration	482:505	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	1	31	theme	anticancer	293:302	arg1	activity					304:311	anticancer activity	293:311	anticancer activity	293:311	Adenosine analogue drugs (such as fludarabine or cladribine) require transporter-mediated uptake into cells and subsequent phosphorylation for anticancer activity.					
29653214	6	32	theme	promising	1257:1265	arg1	carriers					1267:1274	especially promising carriers	1246:1274	especially promising carriers for adenosine analogues	1246:1298	These properties allow us to consider maltose-modified dendrimers as especially promising carriers for adenosine analogues.					
29653214	6	32	theme	promising	1257:1265	arg1	dendrimers					1232:1241	maltose-modified dendrimers	1215:1241	maltose-modified dendrimers	1215:1241	These properties allow us to consider maltose-modified dendrimers as especially promising carriers for adenosine analogues.					
29653214	1	33	theme	Adenosine	150:158	arg1	drugs					169:173	Adenosine analogue drugs	150:173	Adenosine analogue drugs (such as fludarabine or cladribine)	150:209	Adenosine analogue drugs (such as fludarabine or cladribine) require transporter-mediated uptake into cells and subsequent phosphorylation for anticancer activity.					
29653214	3	34	theme	thermodynamic	542:554	arg1	nucleotide					729:738	a model adenosine nucleotide	711:738	a model adenosine nucleotide	711:738	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	34	theme	thermodynamic	542:554	arg1	stoichiometry					524:536	the stoichiometry	520:536	the stoichiometry	520:536	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	34	theme	thermodynamic	542:554	arg1	parameters					556:565	thermodynamic parameters	542:565	thermodynamic parameters	542:565	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	0	35	theme	analogue	126:133	arg1	delivery					140:147	adenosine analogue drug delivery	116:147	adenosine analogue drug delivery	116:147	Poly(propyleneimine) glycodendrimers non-covalently bind ATP in a pH- and salt-dependent manner - model studies for adenosine analogue drug delivery.					
29653214	5	36	theme	more	1143:1146	arg1	sites					1156:1160	more binding sites	1143:1160	more binding sites in acidic pH	1143:1173	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	4	37	theme	electrostatic	876:888	arg1	interactions					890:901	electrostatic interactions	876:901	electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively	876:998	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	5	38	from	interactions	1103:1114	arg1	buffer					1127:1132	organic buffer	1119:1132	organic buffer	1119:1132	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	3	39	theme	isothermal	445:454	arg1	calorimetry					466:476	isothermal titration calorimetry	445:476	isothermal titration calorimetry	445:476	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	0	40	theme	adenosine	116:124	arg1	delivery					140:147	adenosine analogue drug delivery	116:147	adenosine analogue drug delivery	116:147	Poly(propyleneimine) glycodendrimers non-covalently bind ATP in a pH- and salt-dependent manner - model studies for adenosine analogue drug delivery.					
29653214	5	41	theme	binding	1148:1154	arg1	sites					1156:1160	more binding sites	1143:1160	more binding sites in acidic pH	1143:1173	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	0	42	theme	drug	135:138	arg1	delivery					140:147	adenosine analogue drug delivery	116:147	adenosine analogue drug delivery	116:147	Poly(propyleneimine) glycodendrimers non-covalently bind ATP in a pH- and salt-dependent manner - model studies for adenosine analogue drug delivery.					
29653214	3	43	theme	model	713:717	arg1	nucleotide					729:738	a model adenosine nucleotide	711:738	a model adenosine nucleotide	711:738	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	43	theme	model	713:717	arg1	stoichiometry					524:536	the stoichiometry	520:536	the stoichiometry	520:536	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	43	theme	model	713:717	arg1	parameters					556:565	thermodynamic parameters	542:565	thermodynamic parameters	542:565	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	1	44	dep	drugs	169:173	arg1	such					176:179	such	176:179	such	176:179	Adenosine analogue drugs (such as fludarabine or cladribine) require transporter-mediated uptake into cells and subsequent phosphorylation for anticancer activity.					
29653214	2	45	theme	systems	352:358	arg1	application					325:335	application	325:335	application of nanocarrier systems for direct delivery of active triphosphate forms	325:407	Therefore, application of nanocarrier systems for direct delivery of active triphosphate forms has been proposed.					
29653214	3	46	theme	adenosine	719:727	arg1	nucleotide					729:738	a model adenosine nucleotide	711:738	a model adenosine nucleotide	711:738	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	46	theme	adenosine	719:727	arg1	stoichiometry					524:536	the stoichiometry	520:536	the stoichiometry	520:536	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	46	theme	adenosine	719:727	arg1	parameters					556:565	thermodynamic parameters	542:565	thermodynamic parameters	542:565	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	2	47	theme	nanocarrier	340:350	arg1	systems					352:358	nanocarrier systems	340:358	nanocarrier systems	340:358	Therefore, application of nanocarrier systems for direct delivery of active triphosphate forms has been proposed.					
29653214	3	48	theme	increased	672:680	arg1	biocompatibility					682:697	increased biocompatibility	672:697	increased biocompatibility	672:697	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	4	49	dep	ability	781:787	arg1	interact					804:811	interact	804:811	to efficiently interact with nucleoside triphosphates	789:841	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	4	49	dep	ability	781:787	arg1	form					850:853	form	850:853	to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively	847:998	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	5	50	dep	composition	1080:1090	arg1	interactions					1103:1114	strongest interactions	1093:1114	strongest interactions in organic buffer	1093:1132	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	2	51	theme	active	383:388	arg1	forms					403:407	active triphosphate forms	383:407	active triphosphate forms	383:407	Therefore, application of nanocarrier systems for direct delivery of active triphosphate forms has been proposed.					
29653214	3	52	dep	dendrimers	627:636	arg1	sugar-modified					653:666	sugar-modified	653:666	sugar-modified	653:666	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	3	52	dep	dendrimers	627:636	arg1	unmodified					639:648	unmodified	639:648	unmodified	639:648	Here, we applied isothermal titration calorimetry and zeta potential titration to determine the stoichiometry and thermodynamic parameters of interactions between 4th generation poly(propyleneimine) dendrimers (unmodified or sugar-modified for increased biocompatibility) and ATP as a model adenosine nucleotide.					
29653214	1	53	theme	transporter-mediated	219:238	arg1	uptake					240:245	transporter-mediated uptake	219:245	transporter-mediated uptake into cells	219:256	Adenosine analogue drugs (such as fludarabine or cladribine) require transporter-mediated uptake into cells and subsequent phosphorylation for anticancer activity.					
29653214	4	54	theme	stable	855:860	arg1	complexes					862:870	stable complexes	855:870	stable complexes	855:870	We showed that glycodendrimers have the ability to efficiently interact with nucleoside triphosphates and to form stable complexes via electrostatic interactions between the ionized phosphate and amino groups on the nucleotide and the dendrimer, respectively.					
29653214	5	55	dep	spontaneous	1029:1039	arg1	enthalpy-driven					1042:1056	enthalpy-driven	1042:1056	enthalpy-driven	1042:1056	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	5	55	dep	spontaneous	1029:1039	arg1	spontaneous					1029:1039	spontaneous	1029:1039	spontaneous	1029:1039	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	5	55	dep	spontaneous	1029:1039	arg1	process					1018:1024	The complexation process	1001:1024	The complexation process	1001:1024	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
29653214	5	56	from	sites	1156:1160	arg1	pH					1172:1173	acidic pH	1165:1173	acidic pH	1165:1173	The complexation process is spontaneous, enthalpy-driven and depends on buffer composition (strongest interactions in organic buffer) and pH (more binding sites in acidic pH).					
31162880	5	0	with	complexes	839:847	arg1	EEO					854:856	EEO	854:856	EEO	854:856	Therefore, β-CD can be used to form inclusion complexes with EEO to expand its potential applications in the food and drug industries.					
31162880	3	1	theme	physical	468:475	arg1	mixture					477:483	the physical mixture	464:483	the physical mixture	464:483	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	7	2	theme	constituent	1050:1060	arg1	composition					1062:1072	constituent composition	1050:1072	constituent composition	1050:1072	The optimized extraction, constituent composition, and encapsulation of EEO in β-CD were investigated in this study.					
31162880	7	3	theme	EEO	1096:1098	arg1	extraction					1038:1047	The optimized extraction	1024:1047	The optimized extraction	1024:1047	The optimized extraction, constituent composition, and encapsulation of EEO in β-CD were investigated in this study.					
31162880	7	3	theme	EEO	1096:1098	arg1	composition					1062:1072	constituent composition	1050:1072	constituent composition	1050:1072	The optimized extraction, constituent composition, and encapsulation of EEO in β-CD were investigated in this study.					
31162880	7	3	theme	EEO	1096:1098	arg1	encapsulation					1079:1091	encapsulation	1079:1091	encapsulation of EEO in β-CD	1079:1106	The optimized extraction, constituent composition, and encapsulation of EEO in β-CD were investigated in this study.					
31162880	1	4	dep	β-cyclodextrin	223:236	arg1	oil					284:286	essential oil	274:286	essential oil (EEO)	274:292	The purpose of this study was to produce and characterize an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO), and to evaluate its antioxidant properties.					
31162880	1	4	dep	β-cyclodextrin	223:236	arg1	EEO					289:291	EEO	289:291	EEO	289:291	The purpose of this study was to produce and characterize an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO), and to evaluate its antioxidant properties.					
31162880	7	5	from	extraction	1038:1047	arg1	β-CD					1103:1106	β-CD	1103:1106	β-CD	1103:1106	The optimized extraction, constituent composition, and encapsulation of EEO in β-CD were investigated in this study.					
31162880	8	6	theme	drug	1347:1350	arg1	industries					1352:1361	the food and drug industries	1334:1361	the food and drug industries	1334:1361	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	1	7	theme	antioxidant	315:325	arg1	properties					327:336	its antioxidant properties	311:336	its antioxidant properties	311:336	The purpose of this study was to produce and characterize an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO), and to evaluate its antioxidant properties.					
31162880	8	8	theme	EEO	1327:1329	arg1	application					1312:1322	the application	1308:1322	the application of EEO in the food and drug industries	1308:1361	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	3	9	from	differences	521:531	arg1	morphologies					566:577	morphologies	566:577	morphologies	566:577	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	3	9	from	differences	521:531	arg1	stabilities					550:560	their thermal stabilities	536:560	their thermal stabilities	536:560	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	1	10	theme	Exocarpium	249:258	arg1	Grandis					266:272	Exocarpium Citri Grandis	249:272	Exocarpium Citri Grandis	249:272	The purpose of this study was to produce and characterize an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO), and to evaluate its antioxidant properties.					
31162880	6	11	from	oil	997:999	arg1	rich					979:982	rich	979:982	rich	979:982	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	4	12	theme	inclusion	679:687	arg1	complex					689:695	the β-CD-EEO inclusion complex	666:695	the β-CD-EEO inclusion complex	666:695	Complexed with β-CD, the β-CD-EEO inclusion complex showed a higher stability and antioxidant activity when compared with physical mixture and EEO.					
31162880	4	13	theme	β-CD-EEO	670:677	arg1	complex					689:695	the β-CD-EEO inclusion complex	666:695	the β-CD-EEO inclusion complex	666:695	Complexed with β-CD, the β-CD-EEO inclusion complex showed a higher stability and antioxidant activity when compared with physical mixture and EEO.					
31162880	5	14	used	used	816:819	arg2	β-CD					804:807	β-CD	804:807	β-CD	804:807	Therefore, β-CD can be used to form inclusion complexes with EEO to expand its potential applications in the food and drug industries.					
31162880	3	15	theme	inclusion	494:502	arg1	complex					504:510	the inclusion complex	490:510	the inclusion complex	490:510	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	5	16	theme	inclusion	829:837	arg1	complexes					839:847	inclusion complexes	829:847	inclusion complexes with EEO	829:856	Therefore, β-CD can be used to form inclusion complexes with EEO to expand its potential applications in the food and drug industries.					
31162880	5	17	theme	potential	872:880	arg1	applications					882:893	its potential applications	868:893	its potential applications in the food and drug industries	868:925	Therefore, β-CD can be used to form inclusion complexes with EEO to expand its potential applications in the food and drug industries.					
31162880	6	18	from	ingredients	1011:1021	arg1	rich					979:982	rich	979:982	rich	979:982	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	2	19	theme	EEO	365:367	arg1	compounds					352:360	The volatile compounds	339:360	The volatile compounds of EEO	339:367	The volatile compounds of EEO were characterized by gas chromatography-mass spectrometer.					
31162880	8	20	theme	practical	1284:1292	arg1	knowledge					1294:1302	both fundamental and practical knowledge	1263:1302	both fundamental and practical knowledge	1263:1302	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	7	21	from	encapsulation	1079:1091	arg1	β-CD					1103:1106	β-CD	1103:1106	β-CD	1103:1106	The optimized extraction, constituent composition, and encapsulation of EEO in β-CD were investigated in this study.					
31162880	6	22	from	rich	979:982	arg1	oil					997:999	essential oil	987:999	essential oil	987:999	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	6	22	from	rich	979:982	arg1	ingredients					1011:1021	other ingredients	1005:1021	other ingredients	1005:1021	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	7	23	from	composition	1062:1072	arg1	β-CD					1103:1106	β-CD	1103:1106	β-CD	1103:1106	The optimized extraction, constituent composition, and encapsulation of EEO in β-CD were investigated in this study.					
31162880	3	24	theme	thermal	542:548	arg1	stabilities					550:560	their thermal stabilities	536:560	their thermal stabilities	536:560	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	0	25	theme	Inclusion	0:8	arg1	Complex					10:16	Inclusion Complex	0:16	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.	0:134	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	6	26	theme	Exocarpium	951:960	arg1	Grandis					968:974	Exocarpium Citri Grandis	951:974	Exocarpium Citri Grandis	951:974	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	5	27	from	applications	882:893	arg1	industries					916:925	the food and drug industries	898:925	the food and drug industries	898:925	Therefore, β-CD can be used to form inclusion complexes with EEO to expand its potential applications in the food and drug industries.					
31162880	5	28	theme	drug	911:914	arg1	industries					916:925	the food and drug industries	898:925	the food and drug industries	898:925	Therefore, β-CD can be used to form inclusion complexes with EEO to expand its potential applications in the food and drug industries.					
31162880	8	29	theme	EEO	1243:1245	arg1	stability					1230:1238	stability	1230:1238	stability	1230:1238	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	8	29	theme	EEO	1243:1245	arg1	activity					1217:1224	the antioxidant activity	1201:1224	the antioxidant activity	1201:1224	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	0	30	theme	Exocarpium	21:30	arg1	Oil					56:58	Exocarpium Citri Grandis Essential Oil	21:58	Exocarpium Citri Grandis Essential Oil	21:58	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	1	31	theme	essential	274:282	arg1	oil					284:286	essential oil	274:286	essential oil (EEO)	274:292	The purpose of this study was to produce and characterize an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO), and to evaluate its antioxidant properties.					
31162880	1	31	theme	essential	274:282	arg1	EEO					289:291	EEO	289:291	EEO	289:291	The purpose of this study was to produce and characterize an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO), and to evaluate its antioxidant properties.					
31162880	6	32	dep	APPLICATION	938:948	arg1	rich					979:982	rich	979:982	rich	979:982	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	2	33	theme	chromatography-mass	395:413	arg1	spectrometer					415:426	gas chromatography-mass spectrometer	391:426	gas chromatography-mass spectrometer	391:426	The volatile compounds of EEO were characterized by gas chromatography-mass spectrometer.					
31162880	8	34	theme	food	1338:1341	arg1	industries					1352:1361	the food and drug industries	1334:1361	the food and drug industries	1334:1361	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	0	35	theme	Antioxidant	114:124	arg1	Activity					126:133	Antioxidant Activity	114:133	Antioxidant Activity	114:133	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	3	36	theme	inclusion	626:634	arg1	complex					636:642	the β-CD-EEO inclusion complex	613:642	the β-CD-EEO inclusion complex	613:642	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	1	37	theme	study	156:160	arg1	purpose					140:146	The purpose	136:146	The purpose of this study	136:160	The purpose of this study was to produce and characterize an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO), and to evaluate its antioxidant properties.					
31162880	2	38	theme	gas	391:393	arg1	spectrometer					415:426	gas chromatography-mass spectrometer	391:426	gas chromatography-mass spectrometer	391:426	The volatile compounds of EEO were characterized by gas chromatography-mass spectrometer.					
31162880	0	39	theme	Grandis	38:44	arg1	Oil					56:58	Exocarpium Citri Grandis Essential Oil	21:58	Exocarpium Citri Grandis Essential Oil	21:58	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	6	40	theme	PRACTICAL	928:936	arg1	APPLICATION					938:948	PRACTICAL APPLICATION	928:948	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.	928:1022	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	7	41	theme	optimized	1028:1036	arg1	extraction					1038:1047	The optimized extraction	1024:1047	The optimized extraction	1024:1047	The optimized extraction, constituent composition, and encapsulation of EEO in β-CD were investigated in this study.					
31162880	3	42	theme	complex	636:642	arg1	formation					600:608	the formation	596:608	the formation of the β-CD-EEO inclusion complex	596:642	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	1	43	theme	inclusion	197:205	arg1	complex					207:213	an inclusion complex	194:213	an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO)	194:292	The purpose of this study was to produce and characterize an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO), and to evaluate its antioxidant properties.					
31162880	3	44	dep	β-CD	449:452	arg1	the					445:447	the	445:447	the	445:447	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	8	45	theme	encapsulation	1169:1181	arg1	process					1183:1189	the encapsulation process	1165:1189	the encapsulation process	1165:1189	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	6	46	theme	other	1005:1009	arg1	ingredients					1011:1021	other ingredients	1005:1021	other ingredients	1005:1021	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	0	47	theme	Oil	56:58	arg1	Complex					10:16	Inclusion Complex	0:16	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.	0:134	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	3	48	theme	β-CD-EEO	617:624	arg1	complex					636:642	the β-CD-EEO inclusion complex	613:642	the β-CD-EEO inclusion complex	613:642	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	8	49	theme	fundamental	1268:1278	arg1	knowledge					1294:1302	both fundamental and practical knowledge	1263:1302	both fundamental and practical knowledge	1263:1302	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	0	50	theme	Essential	46:54	arg1	Oil					56:58	Exocarpium Citri Grandis Essential Oil	21:58	Exocarpium Citri Grandis Essential Oil	21:58	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	1	51	dep	Exocarpium	249:258	arg1	Citri					260:264	Citri	260:264	Citri	260:264	The purpose of this study was to produce and characterize an inclusion complex between β-cyclodextrin (β-CD) and Exocarpium Citri Grandis essential oil (EEO), and to evaluate its antioxidant properties.					
31162880	0	52	with	Complex	10:16	arg1	β-Cyclodextrin					65:78	β-Cyclodextrin	65:78	β-Cyclodextrin	65:78	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	3	53	theme	mixture	477:483	arg1	comparison					431:440	A comparison	429:440	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex	429:510	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	0	54	dep	Exocarpium	21:30	arg1	Citri					32:36	Citri	32:36	Citri	32:36	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	3	55	theme	EEO	455:457	arg1	comparison					431:440	A comparison	429:440	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex	429:510	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	3	56	with	comparison	431:440	arg1	complex					504:510	the inclusion complex	490:510	the inclusion complex	490:510	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	6	57	dep	Exocarpium	951:960	arg1	Citri					962:966	Citri	962:966	Citri	962:966	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	4	58	theme	physical	767:774	arg1	mixture					776:782	physical mixture	767:782	physical mixture	767:782	Complexed with β-CD, the β-CD-EEO inclusion complex showed a higher stability and antioxidant activity when compared with physical mixture and EEO.					
31162880	4	59	theme	antioxidant	727:737	arg1	activity					739:746	antioxidant activity	727:746	antioxidant activity	727:746	Complexed with β-CD, the β-CD-EEO inclusion complex showed a higher stability and antioxidant activity when compared with physical mixture and EEO.					
31162880	5	60	theme	food	902:905	arg1	industries					916:925	the food and drug industries	898:925	the food and drug industries	898:925	Therefore, β-CD can be used to form inclusion complexes with EEO to expand its potential applications in the food and drug industries.					
31162880	8	61	from	EEO	1327:1329	arg1	industries					1352:1361	the food and drug industries	1334:1361	the food and drug industries	1334:1361	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	4	62	theme	higher	706:711	arg1	stability					713:721	a higher stability	704:721	a higher stability	704:721	Complexed with β-CD, the β-CD-EEO inclusion complex showed a higher stability and antioxidant activity when compared with physical mixture and EEO.					
31162880	0	63	dep	Complex	10:16	arg1	Stability					99:107	Stability	99:107	Stability	99:107	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	0	63	dep	Complex	10:16	arg1	Characterization					81:96	Characterization	81:96	Characterization	81:96	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	0	63	dep	Complex	10:16	arg1	Activity					126:133	Antioxidant Activity	114:133	Antioxidant Activity	114:133	Inclusion Complex of Exocarpium Citri Grandis Essential Oil with β-Cyclodextrin: Characterization, Stability, and Antioxidant Activity.					
31162880	3	64	theme	β-CD	449:452	arg1	comparison					431:440	A comparison	429:440	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex	429:510	A comparison of the β-CD, EEO, and the physical mixture with the inclusion complex revealed differences in their thermal stabilities and morphologies, which confirmed the formation of the β-CD-EEO inclusion complex.					
31162880	2	65	theme	volatile	343:350	arg1	compounds					352:360	The volatile compounds	339:360	The volatile compounds of EEO	339:367	The volatile compounds of EEO were characterized by gas chromatography-mass spectrometer.					
31162880	6	66	theme	essential	987:995	arg1	oil					997:999	essential oil	987:999	essential oil	987:999	PRACTICAL APPLICATION: Exocarpium Citri Grandis is rich in essential oil and other ingredients.					
31162880	8	67	theme	antioxidant	1205:1215	arg1	activity					1217:1224	the antioxidant activity	1201:1224	the antioxidant activity	1201:1224	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	8	68	from	application	1312:1322	arg1	industries					1352:1361	the food and drug industries	1334:1361	the food and drug industries	1334:1361	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	8	69	dep	increased	1191:1199	arg1	provides					1254:1261	provides	1254:1261	provides both fundamental and practical knowledge for the application of EEO in the food and drug industries	1254:1361	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
31162880	8	70	from	industries	1352:1361	arg1	application					1312:1322	the application	1308:1322	the application of EEO in the food and drug industries	1308:1361	The results showed that the encapsulation process increased the antioxidant activity and stability of EEO, which provides both fundamental and practical knowledge for the application of EEO in the food and drug industries.					
30857962	3	0	theme	MCS-GA	526:531	arg1	properties					508:517	The physicochemical properties	488:517	The physicochemical properties of the MCS-GA	488:531	The physicochemical properties of the MCS-GA were investigated using FTIR, SEM, TEM, XRD, BET, and VSM techniques.					
30857962	7	1	theme	CV	1410:1411	arg1	adsorption					1413:1422	CV adsorption	1410:1422	CV adsorption	1410:1422	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	8	2	theme	real	1579:1582	arg1	terms					1584:1588	real terms	1579:1588	real terms	1579:1588	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	3	3	theme	SEM	563:565	arg1	techniques					591:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	The physicochemical properties of the MCS-GA were investigated using FTIR, SEM, TEM, XRD, BET, and VSM techniques.					
30857962	7	4	from	nature	1468:1473	arg1	feasible					1427:1434	feasible	1427:1434	feasible	1427:1434	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	1	5	theme	Magnetic	122:129	arg1	separation					131:140	Magnetic separation	122:140	Magnetic separation of toxic dyes	122:154	Magnetic separation of toxic dyes has become a potential and effective method in wastewater treatments.					
30857962	2	6	theme	facile	249:254	arg1	approach					300:307	a facile in situ one step co-precipitation synthetic approach	247:307	a facile in situ one step co-precipitation synthetic approach	247:307	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	3	7	theme	FTIR	557:560	arg1	techniques					591:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	The physicochemical properties of the MCS-GA were investigated using FTIR, SEM, TEM, XRD, BET, and VSM techniques.					
30857962	8	8	theme	dyes	1645:1648	arg1	removal					1650:1656	dyes removal	1645:1656	dyes removal from the textile wastewater	1645:1684	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	7	9	dep	ΔH°	1298:1300	arg1	-13.4872 kJ mol-1					1345:1361	-13.4872 kJ mol-1	1345:1361	-13.4872 kJ mol-1	1345:1361	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	7	9	dep	ΔH°	1298:1300	arg1	ΔG°					1329:1331	ΔG°	1329:1331	ΔG°	1329:1331	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	7	9	dep	ΔH°	1298:1300	arg1	690.609-896.006 kJ mol-1					1303:1326	690.609-896.006 kJ mol-1	1303:1326	690.609-896.006 kJ mol-1	1303:1326	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	7	9	dep	ΔH°	1298:1300	arg1	0.168-0.232 kJ mol-1 K-1					1369:1392	0.168-0.232 kJ mol-1 K-1	1369:1392	0.168-0.232 kJ mol-1 K-1	1369:1392	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	7	9	dep	ΔH°	1298:1300	arg1	ΔS°					1364:1366	ΔS°	1364:1366	ΔS°	1364:1366	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	6	10	dep	kinetics	1238:1245	arg1	R2					1248:1249	R2	1248:1249	R2: 0.960	1248:1256	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	4	11	theme	dye	839:841	arg1	concentration					843:855	initial dye concentration	831:855	initial dye concentration	831:855	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	6	12	theme	kinetic	1112:1118	arg1	data					1120:1123	The adsorption equilibrium and kinetic data	1081:1123	data	1120:1123	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	6	13	dep	R2	1248:1249	arg1	0.960					1252:1256	0.960	1252:1256	0.960	1252:1256	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	4	14	theme	3-factors	615:623	arg1	design					643:648	3-factors central composite design	615:648	3-factors central composite design	615:648	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	8	15	from	regeneration	1536:1547	arg1	terms					1584:1588	real terms	1579:1588	real terms	1579:1588	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	8	16	dep	efficiency	1519:1528	arg1	the					1505:1507	the	1505:1507	the	1505:1507	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	3	17	theme	TEM	568:570	arg1	techniques					591:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	The physicochemical properties of the MCS-GA were investigated using FTIR, SEM, TEM, XRD, BET, and VSM techniques.					
30857962	8	18	theme	textile	1667:1673	arg1	wastewater					1675:1684	the textile wastewater	1663:1684	the textile wastewater	1663:1684	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	1	19	theme	wastewater	203:212	arg1	treatments					214:223	wastewater treatments	203:223	wastewater treatments	203:223	Magnetic separation of toxic dyes has become a potential and effective method in wastewater treatments.					
30857962	4	20	theme	composite	633:641	arg1	design					643:648	3-factors central composite design	615:648	3-factors central composite design	615:648	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	5	21	theme	60 mg L-1	1023:1031	arg1	pH					980:981	pH	980:981	pH of 11	980:987	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	21	theme	60 mg L-1	1023:1031	arg1	dosage					1044:1049	MCS-GA dosage	1037:1049	MCS-GA dosage of 0.817 g L-1	1037:1064	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	21	theme	60 mg L-1	1023:1031	arg1	concentration					1006:1018	the initial dye concentration	990:1018	the initial dye concentration of 60 mg L-1	990:1031	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	3	22	theme	BET	578:580	arg1	techniques					591:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	The physicochemical properties of the MCS-GA were investigated using FTIR, SEM, TEM, XRD, BET, and VSM techniques.					
30857962	7	23	dep	-13.4872 kJ mol-1	1345:1361	arg1	to					1342:1343	to	1342:1343	to	1342:1343	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	6	24	theme	isotherm	1165:1172	arg1	model					1174:1178	the Langmuir monolayer isotherm model	1142:1178	the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996)	1142:1212	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	6	25	theme	order	1232:1236	arg1	kinetics					1238:1245	pseudo-second order kinetics	1218:1245	pseudo-second order kinetics (R2: 0.960)	1218:1257	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	5	26	theme	initial	994:1000	arg1	concentration					1006:1018	the initial dye concentration	990:1018	the initial dye concentration of 60 mg L-1	990:1031	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	2	27	theme	in	256:257	arg1	approach					300:307	a facile in situ one step co-precipitation synthetic approach	247:307	a facile in situ one step co-precipitation synthetic approach	247:307	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	1	28	theme	toxic	145:149	arg1	dyes					151:154	toxic dyes	145:154	toxic dyes	145:154	Magnetic separation of toxic dyes has become a potential and effective method in wastewater treatments.					
30857962	7	29	theme	Thermodynamic	1260:1272	arg1	ΔH°					1298:1300	ΔH°	1298:1300	ΔH°	1298:1300	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	7	29	theme	Thermodynamic	1260:1272	arg1	parameters					1274:1283	Thermodynamic parameters	1260:1283	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1)	1260:1393	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	0	30	theme	dyes	56:59	arg1	mechanism					34:42	mechanism	34:42	mechanism	34:42	Experimental design, modeling and mechanism of cationic dyes biosorption on to magnetic chitosan-lutaraldehyde composite.					
30857962	0	30	theme	dyes	56:59	arg1	modeling					21:28	modeling	21:28	modeling	21:28	Experimental design, modeling and mechanism of cationic dyes biosorption on to magnetic chitosan-lutaraldehyde composite.					
30857962	0	30	theme	dyes	56:59	arg1	design					13:18	Experimental design	0:18	Experimental design	0:18	Experimental design, modeling and mechanism of cationic dyes biosorption on to magnetic chitosan-lutaraldehyde composite.					
30857962	4	31	theme	response	674:681	arg1	methodology					691:701	the response surface methodology	670:701	the response surface methodology (RSM)	670:707	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	4	31	theme	response	674:681	arg1	RSM					704:706	RSM	704:706	RSM	704:706	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	0	32	theme	magnetic	79:86	arg1	composite					111:119	magnetic chitosan-lutaraldehyde composite	79:119	magnetic chitosan-lutaraldehyde composite	79:119	Experimental design, modeling and mechanism of cationic dyes biosorption on to magnetic chitosan-lutaraldehyde composite.					
30857962	5	33	theme	MCS-GA	1037:1042	arg1	dosage					1044:1049	MCS-GA dosage	1037:1049	MCS-GA dosage of 0.817 g L-1	1037:1064	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	2	34	theme	co-precipitation	273:288	arg1	approach					300:307	a facile in situ one step co-precipitation synthetic approach	247:307	a facile in situ one step co-precipitation synthetic approach	247:307	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	2	35	from	solution	478:485	arg1	removal					434:440	the removal	430:440	the removal of Crystal Violet (CV) from aqueous solution	430:485	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	0	36	theme	Experimental	0:11	arg1	design					13:18	Experimental design	0:18	Experimental design	0:18	Experimental design, modeling and mechanism of cationic dyes biosorption on to magnetic chitosan-lutaraldehyde composite.					
30857962	6	37	theme	Langmuir	1146:1153	arg1	model					1174:1178	the Langmuir monolayer isotherm model	1142:1178	the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996)	1142:1212	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	2	38	theme	Crystal	445:451	arg1	CV					461:462	CV	461:462	CV	461:462	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	2	38	theme	Crystal	445:451	arg1	Violet					453:458	Crystal Violet	445:458	Crystal Violet (CV)	445:463	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	5	39	theme	best	938:941	arg1	efficiency					943:952	the best efficiency	934:952	the best efficiency (99.99%)	934:961	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	39	theme	best	938:941	arg1	%					960:960	99.99%	955:960	99.99%	955:960	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	2	40	theme	aqueous	470:476	arg1	solution					478:485	aqueous solution	470:485	aqueous solution	470:485	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	4	41	theme	adsorbent	813:821	arg1	dosage					823:828	adsorbent dosage	813:828	adsorbent dosage	813:828	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	5	42	theme	11	986:987	arg1	pH					980:981	pH	980:981	pH of 11	980:987	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	42	theme	11	986:987	arg1	dosage					1044:1049	MCS-GA dosage	1037:1049	MCS-GA dosage of 0.817 g L-1	1037:1064	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	42	theme	11	986:987	arg1	concentration					1006:1018	the initial dye concentration	990:1018	the initial dye concentration of 60 mg L-1	990:1031	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	8	43	theme	excellent	1509:1517	arg1	efficiency					1519:1528	excellent efficiency	1509:1528	excellent efficiency	1509:1528	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	4	44	theme	independent	774:784	arg1	variables					786:794	independent variables	774:794	independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process	774:878	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	2	45	theme	water-dispersible	328:344	arg1	adsorbent					416:424	adsorbent	416:424	adsorbent	416:424	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	2	45	theme	water-dispersible	328:344	arg1	MCS-GA					392:397	MCS-GA	392:397	MCS-GA	392:397	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	2	45	theme	water-dispersible	328:344	arg1	nanocomposites					376:389	water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites	328:389	water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA)	328:398	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	2	46	used	used	312:315	arg2	approach					300:307	a facile in situ one step co-precipitation synthetic approach	247:307	a facile in situ one step co-precipitation synthetic approach	247:307	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	6	47	theme	pseudo-second	1218:1230	arg1	kinetics					1238:1245	pseudo-second order kinetics	1218:1245	pseudo-second order kinetics (R2: 0.960)	1218:1257	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	8	48	theme	acceptable	1553:1562	arg1	performance					1564:1574	acceptable performance	1553:1574	acceptable performance	1553:1574	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	2	49	theme	synthetic	290:298	arg1	approach					300:307	a facile in situ one step co-precipitation synthetic approach	247:307	a facile in situ one step co-precipitation synthetic approach	247:307	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	4	50	theme	adsorption	861:870	arg1	process					872:878	adsorption process	861:878	adsorption process	861:878	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	8	51	from	efficiency	1519:1528	arg1	terms					1584:1588	real terms	1579:1588	real terms	1579:1588	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	2	52	theme	step	268:271	arg1	approach					300:307	a facile in situ one step co-precipitation synthetic approach	247:307	a facile in situ one step co-precipitation synthetic approach	247:307	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	8	53	theme	promising	1621:1629	arg1	MCS-GA					1591:1596	MCS-GA	1591:1596	MCS-GA	1591:1596	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	8	53	theme	promising	1621:1629	arg1	absorbent					1631:1639	a promising absorbent	1619:1639	a promising absorbent for dyes removal from the textile wastewater	1619:1684	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	1	54	theme	potential	169:177	arg1	method					193:198	a potential and effective method	167:198	a potential and effective method in wastewater treatments	167:223	Magnetic separation of toxic dyes has become a potential and effective method in wastewater treatments.					
30857962	1	55	theme	effective	183:191	arg1	method					193:198	a potential and effective method	167:198	a potential and effective method in wastewater treatments	167:223	Magnetic separation of toxic dyes has become a potential and effective method in wastewater treatments.					
30857962	4	56	theme	initial	831:837	arg1	concentration					843:855	initial dye concentration	831:855	initial dye concentration	831:855	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	2	57	dep	in	256:257	arg1	situ					259:262	situ	259:262	situ	259:262	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	6	58	theme	adsorption	1085:1094	arg1	equilibrium					1096:1106	The adsorption equilibrium and kinetic data	1081:1123	equilibrium	1096:1106	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	5	59	dep	follows	971:977	arg1	pH					980:981	pH	980:981	pH of 11	980:987	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	59	dep	follows	971:977	arg1	dosage					1044:1049	MCS-GA dosage	1037:1049	MCS-GA dosage of 0.817 g L-1	1037:1064	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	59	dep	follows	971:977	arg1	concentration					1006:1018	the initial dye concentration	990:1018	the initial dye concentration of 60 mg L-1	990:1031	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	8	60	from	wastewater	1675:1684	arg1	removal					1650:1656	dyes removal	1645:1656	dyes removal from the textile wastewater	1645:1684	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	4	61	dep	pH	809:810	arg1	i.e.					796:799	i.e.	796:799	i.e.	796:799	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	4	62	theme	central	625:631	arg1	design					643:648	3-factors central composite design	615:648	3-factors central composite design	615:648	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	2	63	theme	present	229:235	arg1	research					237:244	present research	229:244	present research	229:244	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	5	64	theme	optimal	897:903	arg1	values					905:910	The optimal values	893:910	The optimal values of the parameters for the best efficiency (99.99%)	893:961	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	4	65	theme	surface	683:689	arg1	methodology					691:701	the response surface methodology	670:701	the response surface methodology (RSM)	670:707	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	4	65	theme	surface	683:689	arg1	RSM					704:706	RSM	704:706	RSM	704:706	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	8	66	from	performance	1564:1574	arg1	terms					1584:1588	real terms	1579:1588	real terms	1579:1588	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	6	67	theme	monolayer	1155:1163	arg1	model					1174:1178	the Langmuir monolayer isotherm model	1142:1178	the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996)	1142:1212	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	5	68	theme	dye	1002:1004	arg1	concentration					1006:1018	the initial dye concentration	990:1018	the initial dye concentration of 60 mg L-1	990:1031	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	3	69	theme	VSM	587:589	arg1	techniques					591:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	FTIR, SEM, TEM, XRD, BET, and VSM techniques	557:600	The physicochemical properties of the MCS-GA were investigated using FTIR, SEM, TEM, XRD, BET, and VSM techniques.					
30857962	1	70	from	method	193:198	arg1	treatments					214:223	wastewater treatments	203:223	wastewater treatments	203:223	Magnetic separation of toxic dyes has become a potential and effective method in wastewater treatments.					
30857962	1	71	theme	dyes	151:154	arg1	separation					131:140	Magnetic separation	122:140	Magnetic separation of toxic dyes	122:154	Magnetic separation of toxic dyes has become a potential and effective method in wastewater treatments.					
30857962	6	72	dep	model	1174:1178	arg1	R2					1203:1204	R2	1203:1204	R2	1203:1204	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	6	72	dep	model	1174:1178	arg1	qmax					1181:1184	qmax	1181:1184	qmax	1181:1184	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	6	73	dep	qmax	1181:1184	arg1	105.467 mg g-1					1187:1200	105.467 mg g-1	1187:1200	105.467 mg g-1	1187:1200	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	6	73	dep	qmax	1181:1184	arg1	0.996					1207:1211	0.996	1207:1211	0.996	1207:1211	The adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 105.467 mg g-1, R2: 0.996) and pseudo-second order kinetics (R2: 0.960).					
30857962	0	74	theme	chitosan-lutaraldehyde	88:109	arg1	composite					111:119	magnetic chitosan-lutaraldehyde composite	79:119	magnetic chitosan-lutaraldehyde composite	79:119	Experimental design, modeling and mechanism of cationic dyes biosorption on to magnetic chitosan-lutaraldehyde composite.					
30857962	4	75	theme	statistical	740:750	arg1	relationships					752:764	the statistical relationships	736:764	the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process	736:878	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	5	76	theme	0.817 g L-1	1054:1064	arg1	pH					980:981	pH	980:981	pH of 11	980:987	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	76	theme	0.817 g L-1	1054:1064	arg1	dosage					1044:1049	MCS-GA dosage	1037:1049	MCS-GA dosage of 0.817 g L-1	1037:1064	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	76	theme	0.817 g L-1	1054:1064	arg1	concentration					1006:1018	the initial dye concentration	990:1018	the initial dye concentration of 60 mg L-1	990:1031	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	5	77	theme	parameters	919:928	arg1	values					905:910	The optimal values	893:910	The optimal values of the parameters for the best efficiency (99.99%)	893:961	The optimal values of the parameters for the best efficiency (99.99%) were as follows: pH of 11, the initial dye concentration of 60 mg L-1 and MCS-GA dosage of 0.817 g L-1, respectively.					
30857962	2	78	theme	Violet	453:458	arg1	removal					434:440	the removal	430:440	the removal of Crystal Violet (CV) from aqueous solution	430:485	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	4	79	theme	initial	801:807	arg1	pH					809:810	initial pH	801:810	initial pH	801:810	5-level and 3-factors central composite design (CCD) combined with the response surface methodology (RSM) was applied to investigate the statistical relationships between independent variables i.e. initial pH, adsorbent dosage, initial dye concentration and adsorption process as response.					
30857962	0	80	theme	cationic	47:54	arg1	dyes					56:59	cationic dyes	47:59	cationic dyes	47:59	Experimental design, modeling and mechanism of cationic dyes biosorption on to magnetic chitosan-lutaraldehyde composite.					
30857962	2	81	theme	Fe3O4/Chitosan/Glutaraldehyde	346:374	arg1	adsorbent					416:424	adsorbent	416:424	adsorbent	416:424	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	2	81	theme	Fe3O4/Chitosan/Glutaraldehyde	346:374	arg1	MCS-GA					392:397	MCS-GA	392:397	MCS-GA	392:397	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	2	81	theme	Fe3O4/Chitosan/Glutaraldehyde	346:374	arg1	nanocomposites					376:389	water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites	328:389	water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA)	328:398	In present research, a facile in situ one step co-precipitation synthetic approach is used to develop water-dispersible Fe3O4/Chitosan/Glutaraldehyde nanocomposites (MCS-GA) as an efficient adsorbent for the removal of Crystal Violet (CV) from aqueous solution.					
30857962	8	82	theme	good	1531:1534	arg1	regeneration					1536:1547	good regeneration	1531:1547	good regeneration	1531:1547	Overall, taking into account the excellent efficiency, good regeneration and acceptable performance in real terms, MCS-GA can be introduced as a promising absorbent for dyes removal from the textile wastewater.					
30857962	3	83	theme	physicochemical	492:506	arg1	properties					508:517	The physicochemical properties	488:517	The physicochemical properties of the MCS-GA	488:531	The physicochemical properties of the MCS-GA were investigated using FTIR, SEM, TEM, XRD, BET, and VSM techniques.					
30857962	7	84	from	feasible	1427:1434	arg1	nature					1468:1473	nature	1468:1473	nature	1468:1473	Thermodynamic parameters (R2 > 0.941, ΔH°: 690.609-896.006 kJ mol-1, ΔG°: -1.6849 to -13.4872 kJ mol-1, ΔS°: 0.168-0.232 kJ mol-1 K-1) also indicated CV adsorption is feasible, spontaneous and endothermic in nature.					
30857962	0	85	dep	design	13:18	arg1	biosorption					61:71	biosorption	61:71	Experimental design, modeling and mechanism of cationic dyes biosorption on to magnetic chitosan-lutaraldehyde composite.	0:120	Experimental design, modeling and mechanism of cationic dyes biosorption on to magnetic chitosan-lutaraldehyde composite.					
31472826	0	0	theme	acid-cellulose	80:93	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	5	1	from	application	918:928	arg1	field					937:941	the field	933:941	the field of smart devices and surface engineering	933:982	These high performance multifunctional hydrogels opens a window for a broad application in the field of smart devices and surface engineering.					
31472826	1	2	theme	composite	160:168	arg1	hydrogel					170:177	a self-healing composite hydrogel	145:177	a self-healing composite hydrogel with antioxidant and antibacterial activities	145:223	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	2	3	theme	ester	496:500	arg1	bonding					502:508	the combined dynamic borate ester bonding	468:508	the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components	468:601	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	1	4	with	hydrogel	170:177	arg1	activities					214:223	antioxidant and antibacterial activities	184:223	activities	214:223	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	5	5	theme	devices	952:958	arg1	field					937:941	the field	933:941	the field of smart devices and surface engineering	933:982	These high performance multifunctional hydrogels opens a window for a broad application in the field of smart devices and surface engineering.					
31472826	1	6	theme	tannic	266:271	arg1	TA					279:280	TA	279:280	TA	279:280	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	1	6	theme	tannic	266:271	arg1	acid					273:276	tannic acid	266:276	tannic acid (TA)	266:281	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	1	6	theme	tannic	266:271	arg1	nanofibrils					244:254	cellulose nanofibrils	234:254	cellulose nanofibrils (CNF)	234:260	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	1	6	theme	tannic	266:271	arg1	additives					297:305	functional additives	286:305	functional additives	286:305	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	0	7	theme	composite	106:114	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	3	8	theme	hydrogels	734:742	arg1	viscoelasticity					715:729	the viscoelasticity	711:729	the viscoelasticity of hydrogels	711:742	The rheological measurements indicated the incorporation of CNF and TA to PB system substantially affected the viscoelasticity of hydrogels.					
31472826	0	9	theme	nanofibril	95:104	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	5	10	theme	smart	946:950	arg1	devices					952:958	smart devices	946:958	smart devices	946:958	These high performance multifunctional hydrogels opens a window for a broad application in the field of smart devices and surface engineering.					
31472826	5	11	theme	broad	912:916	arg1	application					918:928	a broad application	910:928	a broad application in the field of smart devices and surface engineering	910:982	These high performance multifunctional hydrogels opens a window for a broad application in the field of smart devices and surface engineering.					
31472826	5	12	theme	performance	853:863	arg1	hydrogels					881:889	These high performance multifunctional hydrogels	842:889	These high performance multifunctional hydrogels	842:889	These high performance multifunctional hydrogels opens a window for a broad application in the field of smart devices and surface engineering.					
31472826	2	13	theme	external	411:418	arg1	intervention					420:431	any external intervention	407:431	any external intervention	407:431	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	0	14	theme	moldable	33:40	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	0	15	theme	Rapid	0:4	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	1	16	theme	antioxidant	184:194	arg1	activities					214:223	antioxidant and antibacterial activities	184:223	activities	214:223	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	0	17	theme	self-healing	6:17	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	0	18	theme	tannic	73:78	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	2	19	theme	polyvinyl	518:526	arg1	PB					543:544	PB	543:544	PB	543:544	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	2	19	theme	polyvinyl	518:526	arg1	alcohol-borax					528:540	polyvinyl alcohol-borax	518:540	polyvinyl alcohol-borax (PB)	518:545	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	2	20	theme	mechanical	318:327	arg1	stability					329:337	Excellent mechanical stability	308:337	Excellent mechanical stability	308:337	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	2	21	theme	due	461:463	arg1	system					454:459	one system	450:459	one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components	450:601	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	2	22	theme	Excellent	308:316	arg1	stability					329:337	Excellent mechanical stability	308:337	Excellent mechanical stability	308:337	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	1	23	theme	antibacterial	200:212	arg1	activities					214:223	antioxidant and antibacterial activities	184:223	activities	214:223	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	0	24	theme	stretchable	20:30	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	5	25	theme	multifunctional	865:879	arg1	hydrogels					881:889	These high performance multifunctional hydrogels	842:889	These high performance multifunctional hydrogels	842:889	These high performance multifunctional hydrogels opens a window for a broad application in the field of smart devices and surface engineering.					
31472826	3	26	theme	PB	678:679	arg1	system					681:686	PB system	678:686	PB system	678:686	The rheological measurements indicated the incorporation of CNF and TA to PB system substantially affected the viscoelasticity of hydrogels.					
31472826	2	27	theme	borate	489:494	arg1	bonding					502:508	the combined dynamic borate ester bonding	468:508	the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components	468:601	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	1	28	theme	functional	286:295	arg1	nanofibrils					244:254	cellulose nanofibrils	234:254	cellulose nanofibrils (CNF)	234:260	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	1	28	theme	functional	286:295	arg1	acid					273:276	tannic acid	266:276	tannic acid (TA)	266:281	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	1	28	theme	functional	286:295	arg1	additives					297:305	functional additives	286:305	functional additives	286:305	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	2	29	theme	multi-hydrogen	551:564	arg1	bonding					566:572	multi-hydrogen bonding	551:572	multi-hydrogen bonding between different components	551:601	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	2	30	theme	dynamic	481:487	arg1	bonding					502:508	the combined dynamic borate ester bonding	468:508	the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components	468:601	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	3	31	theme	TA	672:673	arg1	incorporation					647:659	the incorporation	643:659	the incorporation of CNF and TA to PB system	643:686	The rheological measurements indicated the incorporation of CNF and TA to PB system substantially affected the viscoelasticity of hydrogels.					
31472826	5	32	theme	surface	964:970	arg1	engineering					972:982	surface engineering	964:982	surface engineering	964:982	These high performance multifunctional hydrogels opens a window for a broad application in the field of smart devices and surface engineering.					
31472826	1	33	theme	cellulose	234:242	arg1	CNF					257:259	CNF	257:259	CNF	257:259	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	1	33	theme	cellulose	234:242	arg1	nanofibrils					244:254	cellulose nanofibrils	234:254	cellulose nanofibrils (CNF)	234:260	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	1	33	theme	cellulose	234:242	arg1	acid					273:276	tannic acid	266:276	tannic acid (TA)	266:281	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	1	33	theme	cellulose	234:242	arg1	additives					297:305	functional additives	286:305	functional additives	286:305	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31472826	0	34	theme	antioxidant	43:53	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	3	35	theme	rheological	608:618	arg1	measurements					620:631	The rheological measurements	604:631	The rheological measurements indicated the incorporation of CNF and TA to PB system	604:686	The rheological measurements indicated the incorporation of CNF and TA to PB system substantially affected the viscoelasticity of hydrogels.					
31472826	4	36	theme	antioxidant	756:766	arg1	properties					786:795	The unique antioxidant and antibacterial properties	745:795	The unique antioxidant and antibacterial properties	745:795	The unique antioxidant and antibacterial properties were achieved due to the complexation of TA.					
31472826	5	37	theme	high	848:851	arg1	hydrogels					881:889	These high performance multifunctional hydrogels	842:889	These high performance multifunctional hydrogels	842:889	These high performance multifunctional hydrogels opens a window for a broad application in the field of smart devices and surface engineering.					
31472826	4	38	theme	unique	749:754	arg1	properties					786:795	The unique antioxidant and antibacterial properties	745:795	The unique antioxidant and antibacterial properties	745:795	The unique antioxidant and antibacterial properties were achieved due to the complexation of TA.					
31472826	3	39	theme	CNF	664:666	arg1	incorporation					647:659	the incorporation	643:659	the incorporation of CNF and TA to PB system	643:686	The rheological measurements indicated the incorporation of CNF and TA to PB system substantially affected the viscoelasticity of hydrogels.					
31472826	4	40	theme	antibacterial	772:784	arg1	properties					786:795	The unique antioxidant and antibacterial properties	745:795	The unique antioxidant and antibacterial properties	745:795	The unique antioxidant and antibacterial properties were achieved due to the complexation of TA.					
31472826	2	41	theme	different	582:590	arg1	components					592:601	different components	582:601	different components	582:601	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	4	42	theme	TA	838:839	arg1	complexation					822:833	the complexation	818:833	the complexation of TA	818:839	The unique antioxidant and antibacterial properties were achieved due to the complexation of TA.					
31472826	2	43	theme	rapid	372:376	arg1	ability					391:397	rapid self-healing ability	372:397	rapid self-healing ability	372:397	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	5	44	theme	engineering	972:982	arg1	field					937:941	the field	933:941	the field of smart devices and surface engineering	933:982	These high performance multifunctional hydrogels opens a window for a broad application in the field of smart devices and surface engineering.					
31472826	0	45	theme	antibacterial	59:71	arg1	hydrogels					116:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels	0:124	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.	0:125	Rapid self-healing, stretchable, moldable, antioxidant and antibacterial tannic acid-cellulose nanofibril composite hydrogels.					
31472826	2	46	theme	self-healing	378:389	arg1	ability					391:397	rapid self-healing ability	372:397	rapid self-healing ability	372:397	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	2	47	theme	combined	472:479	arg1	bonding					502:508	the combined dynamic borate ester bonding	468:508	the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components	468:601	Excellent mechanical stability, moldability, stretchability and rapid self-healing ability without any external intervention were realized in one system due to the combined dynamic borate ester bonding between polyvinyl alcohol-borax (PB) and multi-hydrogen bonding between different components.					
31472826	1	48	theme	self-healing	147:158	arg1	hydrogel					170:177	a self-healing composite hydrogel	145:177	a self-healing composite hydrogel with antioxidant and antibacterial activities	145:223	Here, we designed a self-healing composite hydrogel with antioxidant and antibacterial activities by using cellulose nanofibrils (CNF) and tannic acid (TA) as functional additives.					
31115715	8	0	from	Compatibility	1300:1312	arg1	agglomerates					1357:1368	agglomerates	1357:1368	agglomerates	1357:1368	Compatibility and reduced crystallinity of indapamide in agglomerates were confirmed by spectroscopic and X-ray diffraction studies.					
31115715	7	1	theme	low	1213:1215	arg1	friability					1217:1226	low friability	1213:1226	low friability of agglomerates	1213:1242	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	9	2	theme	drug	1563:1566	arg1	release					1568:1574	the drug release	1559:1574	the drug release (up to 6 h) from agglomerates	1559:1604	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	7	3	theme	crushing	1190:1197	arg1	strength					1199:1206	better crushing strength	1183:1206	better crushing strength	1183:1206	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	5	4	theme	type	966:969	arg1	effect					956:961	the effect	952:961	the effect of type and quantity of polymers on quality attributes of agglomerates	952:1032	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	10	5	used	used	1687:1690	arg2	technique					1655:1663	the developed crystallo-co-agglomeration technique	1614:1663	the developed crystallo-co-agglomeration technique	1614:1663	Hence, the developed crystallo-co-agglomeration technique could be successfully used for the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide.					
31115715	3	6	used	used	655:658	arg2	glycol					638:643	polyethylene glycol 6000	625:648	polyethylene glycol 6000	625:648	Hydroxypropyl methylcellulose 15 cps and polyethylene glycol 6000 were used to impart the desired sphericity, strength, and deformability to agglomerates, respectively.					
31115715	3	6	used	used	655:658	arg2	15 cps					614:619	Hydroxypropyl methylcellulose 15 cps	584:619	Hydroxypropyl methylcellulose 15 cps	584:619	Hydroxypropyl methylcellulose 15 cps and polyethylene glycol 6000 were used to impart the desired sphericity, strength, and deformability to agglomerates, respectively.					
31115715	10	7	theme	extended-release	1751:1766	arg1	agglomerates					1778:1789	directly compressible high-strength extended-release spherical agglomerates	1715:1789	directly compressible high-strength extended-release spherical agglomerates of indapamide	1715:1803	Hence, the developed crystallo-co-agglomeration technique could be successfully used for the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide.					
31115715	3	8	theme	desired	674:680	arg1	sphericity					682:691	the desired sphericity	670:691	the desired sphericity	670:691	Hydroxypropyl methylcellulose 15 cps and polyethylene glycol 6000 were used to impart the desired sphericity, strength, and deformability to agglomerates, respectively.					
31115715	5	9	theme	quantity	975:982	arg1	effect					956:961	the effect	952:961	the effect of type and quantity of polymers on quality attributes of agglomerates	952:1032	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	8	10	theme	spectroscopic	1388:1400	arg1	studies					1424:1430	spectroscopic and X-ray diffraction studies	1388:1430	spectroscopic and X-ray diffraction studies	1388:1430	Compatibility and reduced crystallinity of indapamide in agglomerates were confirmed by spectroscopic and X-ray diffraction studies.					
31115715	10	11	theme	compressible	1724:1735	arg1	agglomerates					1778:1789	directly compressible high-strength extended-release spherical agglomerates	1715:1789	directly compressible high-strength extended-release spherical agglomerates of indapamide	1715:1803	Hence, the developed crystallo-co-agglomeration technique could be successfully used for the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide.					
31115715	4	12	theme	Ethyl	753:757	arg1	10 cps					769:774	Ethyl cellulose 10 cps	753:774	Ethyl cellulose 10 cps	753:774	Ethyl cellulose 10 cps was used to improve the strength of agglomerates and achieve extended release.					
31115715	10	13	theme	indapamide	1794:1803	arg1	agglomerates					1778:1789	directly compressible high-strength extended-release spherical agglomerates	1715:1789	directly compressible high-strength extended-release spherical agglomerates of indapamide	1715:1803	Hence, the developed crystallo-co-agglomeration technique could be successfully used for the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide.					
31115715	7	14	theme	%	1172:1172	arg1	yield					1161:1165	A satisfactory yield	1146:1165	A satisfactory yield (> 97%, wt/wt)	1146:1180	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	7	14	theme	%	1172:1172	arg1	wt/wt					1175:1179	> 97%, wt/wt	1168:1179	wt/wt	1175:1179	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	0	15	theme	Extended-Release	29:44	arg1	System					63:68	High-Strength Extended-Release Multiparticulate System	15:68	High-Strength Extended-Release Multiparticulate System	15:68	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.					
31115715	5	16	theme	effect	956:961	arg1	elucidation					937:947	the elucidation	933:947	the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates	933:1032	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	8	17	theme	indapamide	1343:1352	arg1	Compatibility					1300:1312	Compatibility	1300:1312	Compatibility	1300:1312	Compatibility and reduced crystallinity of indapamide in agglomerates were confirmed by spectroscopic and X-ray diffraction studies.					
31115715	8	17	theme	indapamide	1343:1352	arg1	crystallinity					1326:1338	reduced crystallinity	1318:1338	reduced crystallinity	1318:1338	Compatibility and reduced crystallinity of indapamide in agglomerates were confirmed by spectroscopic and X-ray diffraction studies.					
31115715	4	18	theme	agglomerates	812:823	arg1	strength					800:807	the strength	796:807	the strength of agglomerates	796:823	Ethyl cellulose 10 cps was used to improve the strength of agglomerates and achieve extended release.					
31115715	10	19	theme	agglomerates	1778:1789	arg1	preparation					1700:1710	the preparation	1696:1710	the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide	1696:1803	Hence, the developed crystallo-co-agglomeration technique could be successfully used for the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide.					
31115715	6	20	theme	Prepared	1035:1042	arg1	agglomerates					1044:1055	Prepared agglomerates	1035:1055	Prepared agglomerates	1035:1055	Prepared agglomerates were evaluated for morphological, micromeritic, mechanical, and drug release properties.					
31115715	7	21	theme	satisfactory	1148:1159	arg1	yield					1161:1165	A satisfactory yield	1146:1165	A satisfactory yield (> 97%, wt/wt)	1146:1180	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	7	21	theme	satisfactory	1148:1159	arg1	wt/wt					1175:1179	> 97%, wt/wt	1168:1179	wt/wt	1175:1179	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	1	22	theme	unit	170:173	arg1	operations					175:184	unit operations	170:184	unit operations	170:184	The number of unit operations to be followed in the preparation of tablets was cumbersome and may introduce material as well as process-related critical parameters which may negatively affect the quality of final formulation.					
31115715	5	23	theme	agglomerates	1021:1032	arg1	attributes					1007:1016	quality attributes	999:1016	quality attributes of agglomerates	999:1032	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	5	24	theme	experiment	865:874	arg1	Design					855:860	Design	855:860	Design of experiment (rotatable central composite design)	855:911	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	1	25	theme	final	363:367	arg1	formulation					369:379	final formulation	363:379	final formulation	363:379	The number of unit operations to be followed in the preparation of tablets was cumbersome and may introduce material as well as process-related critical parameters which may negatively affect the quality of final formulation.					
31115715	0	26	theme	High-Strength	15:27	arg1	System					63:68	High-Strength Extended-Release Multiparticulate System	15:68	High-Strength Extended-Release Multiparticulate System	15:68	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.					
31115715	5	27	theme	polymers	987:994	arg1	type					966:969	type	966:969	type	966:969	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	5	27	theme	polymers	987:994	arg1	quantity					975:982	quantity	975:982	quantity	975:982	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	6	28	theme	drug	1121:1124	arg1	properties					1134:1143	drug release properties	1121:1143	drug release properties	1121:1143	Prepared agglomerates were evaluated for morphological, micromeritic, mechanical, and drug release properties.					
31115715	5	29	theme	rotatable	877:885	arg1	design					905:910	rotatable central composite design	877:910	rotatable central composite design	877:910	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	5	29	theme	rotatable	877:885	arg1	experiment					865:874	experiment	865:874	experiment (rotatable central composite design)	865:911	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	5	30	theme	quality	999:1005	arg1	attributes					1007:1016	quality attributes	999:1016	quality attributes of agglomerates	999:1032	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	0	31	theme	Central	130:136	arg1	Design					148:153	Central Composite Design	130:153	Central Composite Design	130:153	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.					
31115715	1	32	theme	critical	300:307	arg1	parameters					309:318	process-related critical parameters	284:318	material as well as process-related critical parameters which may negatively affect the quality of final formulation	264:379	The number of unit operations to be followed in the preparation of tablets was cumbersome and may introduce material as well as process-related critical parameters which may negatively affect the quality of final formulation.					
31115715	9	33	theme	miniscular	1450:1459	arg1	form					1468:1471	the miniscular dosage form	1446:1471	the miniscular dosage form	1446:1471	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	5	34	theme	central	887:893	arg1	design					905:910	rotatable central composite design	877:910	rotatable central composite design	877:910	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	5	34	theme	central	887:893	arg1	experiment					865:874	experiment	865:874	experiment (rotatable central composite design)	865:911	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	7	35	theme	handling	1274:1281	arg1	characteristics					1283:1297	good processing and handling characteristics	1254:1297	good processing and handling characteristics	1254:1297	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	9	36	theme	form	1468:1471	arg1	factors					1513:1519	the key factors	1505:1519	the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates	1505:1604	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	9	36	theme	form	1468:1471	arg1	Formation					1433:1441	Formation	1433:1441	Formation of the miniscular dosage form and hydrophobicity of talc	1433:1498	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	5	37	from	attributes	1007:1016	arg1	type					966:969	type	966:969	type	966:969	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	5	37	from	attributes	1007:1016	arg1	quantity					975:982	quantity	975:982	quantity	975:982	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	2	38	theme	spherical	490:498	arg1	agglomerates					500:511	directly compressible, high-strength extended-release spherical agglomerates	436:511	directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide	436:541	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	7	39	theme	processing	1259:1268	arg1	characteristics					1283:1297	good processing and handling characteristics	1254:1297	good processing and handling characteristics	1254:1297	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	9	40	theme	hydrophobicity	1477:1490	arg1	factors					1513:1519	the key factors	1505:1519	the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates	1505:1604	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	9	40	theme	hydrophobicity	1477:1490	arg1	Formation					1433:1441	Formation	1433:1441	Formation of the miniscular dosage form and hydrophobicity of talc	1433:1498	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	2	41	dep	compressible	445:456	arg1	high-strength					459:471	high-strength	459:471	high-strength	459:471	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	2	42	theme	compressible	445:456	arg1	agglomerates					500:511	directly compressible, high-strength extended-release spherical agglomerates	436:511	directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide	436:541	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	5	43	theme	composite	895:903	arg1	design					905:910	rotatable central composite design	877:910	rotatable central composite design	877:910	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	5	43	theme	composite	895:903	arg1	experiment					865:874	experiment	865:874	experiment (rotatable central composite design)	865:911	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	9	44	theme	talc	1495:1498	arg1	hydrophobicity					1477:1490	hydrophobicity	1477:1490	hydrophobicity	1477:1490	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	9	44	theme	talc	1495:1498	arg1	form					1468:1471	the miniscular dosage form	1446:1471	the miniscular dosage form	1446:1471	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	0	45	theme	Multiparticulate	46:61	arg1	System					63:68	High-Strength Extended-Release Multiparticulate System	15:68	High-Strength Extended-Release Multiparticulate System	15:68	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.					
31115715	2	46	theme	containing	521:530	arg1	indapamide					532:541	talc containing indapamide	516:541	talc containing indapamide	516:541	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	9	47	dep	release	1568:1574	arg1	6 h					1583:1585	6 h	1583:1585	6 h	1583:1585	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	10	48	theme	crystallo-co-agglomeration	1628:1653	arg1	technique					1655:1663	the developed crystallo-co-agglomeration technique	1614:1663	the developed crystallo-co-agglomeration technique	1614:1663	Hence, the developed crystallo-co-agglomeration technique could be successfully used for the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide.					
31115715	9	49	dep	6 h	1583:1585	arg1	to					1580:1581	to	1580:1581	to	1580:1581	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	2	50	theme	present	404:410	arg1	research					412:419	the present research	400:419	the present research	400:419	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	3	51	theme	methylcellulose	598:612	arg1	15 cps					614:619	Hydroxypropyl methylcellulose 15 cps	584:619	Hydroxypropyl methylcellulose 15 cps	584:619	Hydroxypropyl methylcellulose 15 cps and polyethylene glycol 6000 were used to impart the desired sphericity, strength, and deformability to agglomerates, respectively.					
31115715	1	52	theme	process-related	284:298	arg1	parameters					309:318	process-related critical parameters	284:318	material as well as process-related critical parameters which may negatively affect the quality of final formulation	264:379	The number of unit operations to be followed in the preparation of tablets was cumbersome and may introduce material as well as process-related critical parameters which may negatively affect the quality of final formulation.					
31115715	7	53	theme	better	1183:1188	arg1	strength					1199:1206	better crushing strength	1183:1206	better crushing strength	1183:1206	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	0	54	with	Technique	100:108	arg1	Integration					115:125	Integration	115:125	Integration of Central Composite Design	115:153	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.					
31115715	8	55	from	crystallinity	1326:1338	arg1	agglomerates					1357:1368	agglomerates	1357:1368	agglomerates	1357:1368	Compatibility and reduced crystallinity of indapamide in agglomerates were confirmed by spectroscopic and X-ray diffraction studies.					
31115715	4	56	theme	cellulose	759:767	arg1	10 cps					769:774	Ethyl cellulose 10 cps	753:774	Ethyl cellulose 10 cps	753:774	Ethyl cellulose 10 cps was used to improve the strength of agglomerates and achieve extended release.					
31115715	10	57	theme	spherical	1768:1776	arg1	agglomerates					1778:1789	directly compressible high-strength extended-release spherical agglomerates	1715:1789	directly compressible high-strength extended-release spherical agglomerates of indapamide	1715:1803	Hence, the developed crystallo-co-agglomeration technique could be successfully used for the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide.					
31115715	10	58	theme	high-strength	1737:1749	arg1	agglomerates					1778:1789	directly compressible high-strength extended-release spherical agglomerates	1715:1789	directly compressible high-strength extended-release spherical agglomerates of indapamide	1715:1803	Hence, the developed crystallo-co-agglomeration technique could be successfully used for the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide.					
31115715	1	59	theme	tablets	223:229	arg1	preparation					208:218	the preparation	204:218	the preparation of tablets	204:229	The number of unit operations to be followed in the preparation of tablets was cumbersome and may introduce material as well as process-related critical parameters which may negatively affect the quality of final formulation.					
31115715	8	60	theme	reduced	1318:1324	arg1	crystallinity					1326:1338	reduced crystallinity	1318:1338	reduced crystallinity	1318:1338	Compatibility and reduced crystallinity of indapamide in agglomerates were confirmed by spectroscopic and X-ray diffraction studies.					
31115715	4	61	used	used	780:783	arg2	10 cps					769:774	Ethyl cellulose 10 cps	753:774	Ethyl cellulose 10 cps	753:774	Ethyl cellulose 10 cps was used to improve the strength of agglomerates and achieve extended release.					
31115715	8	62	theme	diffraction	1412:1422	arg1	studies					1424:1430	spectroscopic and X-ray diffraction studies	1388:1430	spectroscopic and X-ray diffraction studies	1388:1430	Compatibility and reduced crystallinity of indapamide in agglomerates were confirmed by spectroscopic and X-ray diffraction studies.					
31115715	0	63	theme	System	63:68	arg1	Development					0:10	Development	0:10	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.	0:154	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.					
31115715	5	64	from	quantity	975:982	arg1	attributes					1007:1016	quality attributes	999:1016	quality attributes of agglomerates	999:1032	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	1	65	theme	operations	175:184	arg1	number					160:165	The number	156:165	The number of unit operations to be followed in the preparation of tablets	156:229	The number of unit operations to be followed in the preparation of tablets was cumbersome and may introduce material as well as process-related critical parameters which may negatively affect the quality of final formulation.					
31115715	6	66	theme	release	1126:1132	arg1	properties					1134:1143	drug release properties	1121:1143	drug release properties	1121:1143	Prepared agglomerates were evaluated for morphological, micromeritic, mechanical, and drug release properties.					
31115715	1	67	theme	formulation	369:379	arg1	quality					352:358	the quality	348:358	the quality of final formulation	348:379	The number of unit operations to be followed in the preparation of tablets was cumbersome and may introduce material as well as process-related critical parameters which may negatively affect the quality of final formulation.					
31115715	5	68	from	type	966:969	arg1	attributes					1007:1016	quality attributes	999:1016	quality attributes of agglomerates	999:1032	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	2	69	theme	crystallo-co-agglomeration	546:571	arg1	technique					573:581	crystallo-co-agglomeration technique	546:581	crystallo-co-agglomeration technique	546:581	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	0	70	theme	Composite	138:146	arg1	Design					148:153	Central Composite Design	130:153	Central Composite Design	130:153	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.					
31115715	5	71	from	polymers	987:994	arg1	attributes					1007:1016	quality attributes	999:1016	quality attributes of agglomerates	999:1032	Design of experiment (rotatable central composite design) was implemented for the elucidation of the effect of type and quantity of polymers on quality attributes of agglomerates.					
31115715	9	72	theme	dosage	1461:1466	arg1	form					1468:1471	the miniscular dosage form	1446:1471	the miniscular dosage form	1446:1471	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	4	73	theme	extended	837:844	arg1	release					846:852	extended release	837:852	extended release	837:852	Ethyl cellulose 10 cps was used to improve the strength of agglomerates and achieve extended release.					
31115715	2	74	theme	research	412:419	arg1	hypothesis					386:395	The hypothesis	382:395	The hypothesis of the present research	382:419	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	7	75	theme	good	1254:1257	arg1	characteristics					1283:1297	good processing and handling characteristics	1254:1297	good processing and handling characteristics	1254:1297	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	2	76	theme	extended-release	473:488	arg1	agglomerates					500:511	directly compressible, high-strength extended-release spherical agglomerates	436:511	directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide	436:541	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	7	77	theme	agglomerates	1231:1242	arg1	yield					1161:1165	A satisfactory yield	1146:1165	A satisfactory yield (> 97%, wt/wt)	1146:1180	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	7	77	theme	agglomerates	1231:1242	arg1	friability					1217:1226	low friability	1213:1226	low friability of agglomerates	1213:1242	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	7	77	theme	agglomerates	1231:1242	arg1	strength					1199:1206	better crushing strength	1183:1206	better crushing strength	1183:1206	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	7	77	theme	agglomerates	1231:1242	arg1	wt/wt					1175:1179	> 97%, wt/wt	1168:1179	wt/wt	1175:1179	A satisfactory yield (> 97%, wt/wt), better crushing strength, and low friability of agglomerates indicated good processing and handling characteristics.					
31115715	8	78	theme	X-ray	1406:1410	arg1	diffraction					1412:1422	X-ray diffraction	1406:1422	X-ray diffraction	1406:1422	Compatibility and reduced crystallinity of indapamide in agglomerates were confirmed by spectroscopic and X-ray diffraction studies.					
31115715	2	79	theme	indapamide	532:541	arg1	agglomerates					500:511	directly compressible, high-strength extended-release spherical agglomerates	436:511	directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide	436:541	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	0	80	theme	Design	148:153	arg1	Integration					115:125	Integration	115:125	Integration of Central Composite Design	115:153	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.					
31115715	0	81	theme	Crystallo-co-agglomeration	73:98	arg1	Technique					100:108	Crystallo-co-agglomeration Technique	73:108	Crystallo-co-agglomeration Technique with Integration of Central Composite Design	73:153	Development of High-Strength Extended-Release Multiparticulate System by Crystallo-co-agglomeration Technique with Integration of Central Composite Design.					
31115715	9	82	from	agglomerates	1593:1604	arg1	release					1568:1574	the drug release	1559:1574	the drug release (up to 6 h) from agglomerates	1559:1604	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	2	83	theme	talc	516:519	arg1	indapamide					532:541	talc containing indapamide	516:541	talc containing indapamide	516:541	The hypothesis of the present research was to develop directly compressible, high-strength extended-release spherical agglomerates of talc containing indapamide by crystallo-co-agglomeration technique.					
31115715	3	84	theme	Hydroxypropyl	584:596	arg1	15 cps					614:619	Hydroxypropyl methylcellulose 15 cps	584:619	Hydroxypropyl methylcellulose 15 cps	584:619	Hydroxypropyl methylcellulose 15 cps and polyethylene glycol 6000 were used to impart the desired sphericity, strength, and deformability to agglomerates, respectively.					
31115715	9	85	theme	key	1509:1511	arg1	factors					1513:1519	the key factors	1505:1519	the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates	1505:1604	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	9	85	theme	key	1509:1511	arg1	Formation					1433:1441	Formation	1433:1441	Formation of the miniscular dosage form and hydrophobicity of talc	1433:1498	Formation of the miniscular dosage form and hydrophobicity of talc were the key factors observed in controlling and extending the drug release (up to 6 h) from agglomerates.					
31115715	10	86	theme	developed	1618:1626	arg1	technique					1655:1663	the developed crystallo-co-agglomeration technique	1614:1663	the developed crystallo-co-agglomeration technique	1614:1663	Hence, the developed crystallo-co-agglomeration technique could be successfully used for the preparation of directly compressible high-strength extended-release spherical agglomerates of indapamide.					
31115715	3	87	theme	polyethylene	625:636	arg1	glycol					638:643	polyethylene glycol 6000	625:648	polyethylene glycol 6000	625:648	Hydroxypropyl methylcellulose 15 cps and polyethylene glycol 6000 were used to impart the desired sphericity, strength, and deformability to agglomerates, respectively.					
29990535	2	0	from	composition	419:429	arg1	consequence					381:391	a consequence	379:391	a consequence of small changes in their composition	379:429	Their structure can easily break down to form lamellar phases, micelles or rearrange into multilamellar vesicles, as a consequence of small changes in their composition.					
29990535	4	1	theme	unilamellar	691:701	arg1	structures					703:712	unilamellar structures	691:712	unilamellar structures	691:712	Therefore, improving the stability of the vesicles, as well as the control on unilamellar structures, are prerequisites for their wider application range.					
29990535	1	2	theme	catanionic	148:157	arg1	vesicles					159:166	catanionic vesicles	148:166	catanionic vesicles	148:166	The limited stability of catanionic vesicles has discouraged their wide use for encapsulation and controlled release of active substances.					
29990535	1	3	theme	substances	250:259	arg1	release					232:238	controlled release	221:238	controlled release of active substances	221:259	The limited stability of catanionic vesicles has discouraged their wide use for encapsulation and controlled release of active substances.					
29990535	1	3	theme	substances	250:259	arg1	use					195:197	their wide use	184:197	their wide use for encapsulation	184:215	The limited stability of catanionic vesicles has discouraged their wide use for encapsulation and controlled release of active substances.					
29990535	1	4	theme	vesicles	159:166	arg1	stability					135:143	The limited stability	123:143	The limited stability of catanionic vesicles	123:166	The limited stability of catanionic vesicles has discouraged their wide use for encapsulation and controlled release of active substances.					
29990535	4	5	from	control	680:686	arg1	structures					703:712	unilamellar structures	691:712	unilamellar structures	691:712	Therefore, improving the stability of the vesicles, as well as the control on unilamellar structures, are prerequisites for their wider application range.					
29990535	0	6	theme	tunable	94:100	arg1	depot					116:120	a tunable drug delivery depot	92:120	a tunable drug delivery depot	92:120	Stabilization of unilamellar catanionic vesicles induced by β-cyclodextrins: A strategy for a tunable drug delivery depot.					
29990535	4	7	theme	wider	743:747	arg1	range					761:765	their wider application range	737:765	their wider application range	737:765	Therefore, improving the stability of the vesicles, as well as the control on unilamellar structures, are prerequisites for their wider application range.					
29990535	7	8	theme	spectroscopic	1020:1032	arg1	techniques					1034:1043	spectroscopic techniques	1020:1043	spectroscopic techniques	1020:1043	Diffusion and spectroscopic techniques evidenced that when β-cyclodextrins are added, unilamellar structures are stabilized above the multilamellar-unilamellar vesicles critical temperature.					
29990535	2	9	theme	multilamellar	352:364	arg1	vesicles					366:373	multilamellar vesicles	352:373	multilamellar vesicles	352:373	Their structure can easily break down to form lamellar phases, micelles or rearrange into multilamellar vesicles, as a consequence of small changes in their composition.					
29990535	3	10	theme	catanionic	472:481	arg1	vesicles					483:490	catanionic vesicles	472:490	catanionic vesicles	472:490	However, despite the limited stability, catanionic vesicles possess an attractive architecture, which is able to efficiently encapsulate both hydrophobic and hydrophilic molecules.					
29990535	0	11	theme	delivery	107:114	arg1	depot					116:120	a tunable drug delivery depot	92:120	a tunable drug delivery depot	92:120	Stabilization of unilamellar catanionic vesicles induced by β-cyclodextrins: A strategy for a tunable drug delivery depot.					
29990535	6	12	theme	preparation	898:908	arg1	procedures					910:919	sample preparation procedures	891:919	sample preparation procedures	891:919	Molar ratio and sample preparation procedures have been investigated to evaluate the temperature stability of catanionic vesicles.					
29990535	7	13	theme	vesicles	1166:1173	arg1	temperature					1184:1194	the multilamellar-unilamellar vesicles critical temperature	1136:1194	the multilamellar-unilamellar vesicles critical temperature	1136:1194	Diffusion and spectroscopic techniques evidenced that when β-cyclodextrins are added, unilamellar structures are stabilized above the multilamellar-unilamellar vesicles critical temperature.					
29990535	8	14	theme	drug	1287:1290	arg1	applications					1298:1309	drug depot applications	1287:1309	drug depot applications	1287:1309	The results evidence encouraging perspectives for the use of vesicular nanoreservoirs for drug depot applications.					
29990535	5	15	theme	vesicles	865:872	arg1	stabilization					828:840	the stabilization	824:840	the stabilization of SDS/CTAB catanionic vesicles	824:872	This study focuses on the impact of β-cyclodextrins for the stabilization of SDS/CTAB catanionic vesicles.					
29990535	0	16	theme	drug	102:105	arg1	depot					116:120	a tunable drug delivery depot	92:120	a tunable drug delivery depot	92:120	Stabilization of unilamellar catanionic vesicles induced by β-cyclodextrins: A strategy for a tunable drug delivery depot.					
29990535	3	17	contain	possess	492:498	arg1	vesicles					483:490	catanionic vesicles	472:490	catanionic vesicles	472:490	However, despite the limited stability, catanionic vesicles possess an attractive architecture, which is able to efficiently encapsulate both hydrophobic and hydrophilic molecules.					
29990535	3	17	contain	possess	492:498	arg2	architecture					514:525	an attractive architecture	500:525	an attractive architecture	500:525	However, despite the limited stability, catanionic vesicles possess an attractive architecture, which is able to efficiently encapsulate both hydrophobic and hydrophilic molecules.					
29990535	3	17	contain	possess	492:498	arg2	able					537:540	able	537:540	able	537:540	However, despite the limited stability, catanionic vesicles possess an attractive architecture, which is able to efficiently encapsulate both hydrophobic and hydrophilic molecules.					
29990535	7	18	theme	critical	1175:1182	arg1	temperature					1184:1194	the multilamellar-unilamellar vesicles critical temperature	1136:1194	the multilamellar-unilamellar vesicles critical temperature	1136:1194	Diffusion and spectroscopic techniques evidenced that when β-cyclodextrins are added, unilamellar structures are stabilized above the multilamellar-unilamellar vesicles critical temperature.					
29990535	8	19	theme	nanoreservoirs	1268:1281	arg1	use					1251:1253	the use	1247:1253	the use of vesicular nanoreservoirs for drug depot applications	1247:1309	The results evidence encouraging perspectives for the use of vesicular nanoreservoirs for drug depot applications.					
29990535	8	20	theme	vesicular	1258:1266	arg1	nanoreservoirs					1268:1281	vesicular nanoreservoirs	1258:1281	vesicular nanoreservoirs	1258:1281	The results evidence encouraging perspectives for the use of vesicular nanoreservoirs for drug depot applications.					
29990535	8	21	theme	encouraging	1218:1228	arg1	perspectives					1230:1241	encouraging perspectives	1218:1241	encouraging perspectives	1218:1241	The results evidence encouraging perspectives for the use of vesicular nanoreservoirs for drug depot applications.					
29990535	4	22	dep	prerequisites	719:731	arg1	improving					624:632	improving	624:632	improving the stability of the vesicles	624:662	Therefore, improving the stability of the vesicles, as well as the control on unilamellar structures, are prerequisites for their wider application range.					
29990535	4	22	dep	prerequisites	719:731	arg1	control					680:686	the control	676:686	the control on unilamellar structures	676:712	Therefore, improving the stability of the vesicles, as well as the control on unilamellar structures, are prerequisites for their wider application range.					
29990535	5	23	theme	β-cyclodextrins	804:818	arg1	impact					794:799	the impact	790:799	the impact of β-cyclodextrins for the stabilization of SDS/CTAB catanionic vesicles	790:872	This study focuses on the impact of β-cyclodextrins for the stabilization of SDS/CTAB catanionic vesicles.					
29990535	7	24	theme	multilamellar-unilamellar	1140:1164	arg1	vesicles					1166:1173	multilamellar-unilamellar vesicles	1140:1173	the multilamellar-unilamellar vesicles critical temperature	1136:1194	Diffusion and spectroscopic techniques evidenced that when β-cyclodextrins are added, unilamellar structures are stabilized above the multilamellar-unilamellar vesicles critical temperature.					
29990535	2	25	theme	lamellar	308:315	arg1	micelles					325:332	micelles	325:332	micelles	325:332	Their structure can easily break down to form lamellar phases, micelles or rearrange into multilamellar vesicles, as a consequence of small changes in their composition.					
29990535	2	25	theme	lamellar	308:315	arg1	phases					317:322	lamellar phases	308:322	lamellar phases	308:322	Their structure can easily break down to form lamellar phases, micelles or rearrange into multilamellar vesicles, as a consequence of small changes in their composition.					
29990535	1	26	theme	wide	190:193	arg1	use					195:197	their wide use	184:197	their wide use for encapsulation	184:215	The limited stability of catanionic vesicles has discouraged their wide use for encapsulation and controlled release of active substances.					
29990535	0	27	theme	catanionic	29:38	arg1	vesicles					40:47	unilamellar catanionic vesicles	17:47	unilamellar catanionic vesicles induced by β-cyclodextrins	17:74	Stabilization of unilamellar catanionic vesicles induced by β-cyclodextrins: A strategy for a tunable drug delivery depot.					
29990535	5	28	theme	SDS/CTAB	845:852	arg1	vesicles					865:872	SDS/CTAB catanionic vesicles	845:872	SDS/CTAB catanionic vesicles	845:872	This study focuses on the impact of β-cyclodextrins for the stabilization of SDS/CTAB catanionic vesicles.					
29990535	4	29	theme	application	749:759	arg1	range					761:765	their wider application range	737:765	their wider application range	737:765	Therefore, improving the stability of the vesicles, as well as the control on unilamellar structures, are prerequisites for their wider application range.					
29990535	3	30	theme	limited	453:459	arg1	stability					461:469	the limited stability	449:469	the limited stability	449:469	However, despite the limited stability, catanionic vesicles possess an attractive architecture, which is able to efficiently encapsulate both hydrophobic and hydrophilic molecules.					
29990535	0	31	theme	unilamellar	17:27	arg1	vesicles					40:47	unilamellar catanionic vesicles	17:47	unilamellar catanionic vesicles induced by β-cyclodextrins	17:74	Stabilization of unilamellar catanionic vesicles induced by β-cyclodextrins: A strategy for a tunable drug delivery depot.					
29990535	6	32	theme	catanionic	985:994	arg1	vesicles					996:1003	catanionic vesicles	985:1003	catanionic vesicles	985:1003	Molar ratio and sample preparation procedures have been investigated to evaluate the temperature stability of catanionic vesicles.					
29990535	3	33	theme	attractive	503:512	arg1	able					537:540	able	537:540	able	537:540	However, despite the limited stability, catanionic vesicles possess an attractive architecture, which is able to efficiently encapsulate both hydrophobic and hydrophilic molecules.					
29990535	3	33	theme	attractive	503:512	arg1	architecture					514:525	an attractive architecture	500:525	an attractive architecture	500:525	However, despite the limited stability, catanionic vesicles possess an attractive architecture, which is able to efficiently encapsulate both hydrophobic and hydrophilic molecules.					
29990535	4	34	theme	vesicles	655:662	arg1	stability					638:646	the stability	634:646	the stability of the vesicles	634:662	Therefore, improving the stability of the vesicles, as well as the control on unilamellar structures, are prerequisites for their wider application range.					
29990535	2	35	theme	changes	402:408	arg1	consequence					381:391	a consequence	379:391	a consequence of small changes in their composition	379:429	Their structure can easily break down to form lamellar phases, micelles or rearrange into multilamellar vesicles, as a consequence of small changes in their composition.					
29990535	0	36	theme	vesicles	40:47	arg1	Stabilization					0:12	Stabilization	0:12	Stabilization of unilamellar catanionic vesicles induced by β-cyclodextrins: A strategy for a tunable drug delivery depot.	0:121	Stabilization of unilamellar catanionic vesicles induced by β-cyclodextrins: A strategy for a tunable drug delivery depot.					
29990535	8	37	theme	depot	1292:1296	arg1	applications					1298:1309	drug depot applications	1287:1309	drug depot applications	1287:1309	The results evidence encouraging perspectives for the use of vesicular nanoreservoirs for drug depot applications.					
29990535	2	38	theme	small	396:400	arg1	changes					402:408	small changes	396:408	small changes in their composition	396:429	Their structure can easily break down to form lamellar phases, micelles or rearrange into multilamellar vesicles, as a consequence of small changes in their composition.					
29990535	6	39	theme	temperature	960:970	arg1	stability					972:980	the temperature stability	956:980	the temperature stability of catanionic vesicles	956:1003	Molar ratio and sample preparation procedures have been investigated to evaluate the temperature stability of catanionic vesicles.					
29990535	6	40	theme	vesicles	996:1003	arg1	stability					972:980	the temperature stability	956:980	the temperature stability of catanionic vesicles	956:1003	Molar ratio and sample preparation procedures have been investigated to evaluate the temperature stability of catanionic vesicles.					
29990535	6	41	theme	sample	891:896	arg1	procedures					910:919	sample preparation procedures	891:919	sample preparation procedures	891:919	Molar ratio and sample preparation procedures have been investigated to evaluate the temperature stability of catanionic vesicles.					
29990535	7	42	theme	unilamellar	1092:1102	arg1	structures					1104:1113	unilamellar structures	1092:1113	unilamellar structures	1092:1113	Diffusion and spectroscopic techniques evidenced that when β-cyclodextrins are added, unilamellar structures are stabilized above the multilamellar-unilamellar vesicles critical temperature.					
29990535	3	43	theme	hydrophilic	590:600	arg1	molecules					602:610	both hydrophobic and hydrophilic molecules	569:610	both hydrophobic and hydrophilic molecules	569:610	However, despite the limited stability, catanionic vesicles possess an attractive architecture, which is able to efficiently encapsulate both hydrophobic and hydrophilic molecules.					
29990535	2	44	from	consequence	381:391	arg1	composition					419:429	their composition	413:429	their composition	413:429	Their structure can easily break down to form lamellar phases, micelles or rearrange into multilamellar vesicles, as a consequence of small changes in their composition.					
29990535	1	45	theme	controlled	221:230	arg1	release					232:238	controlled release	221:238	controlled release of active substances	221:259	The limited stability of catanionic vesicles has discouraged their wide use for encapsulation and controlled release of active substances.					
29990535	2	46	from	changes	402:408	arg1	composition					419:429	their composition	413:429	their composition	413:429	Their structure can easily break down to form lamellar phases, micelles or rearrange into multilamellar vesicles, as a consequence of small changes in their composition.					
29990535	1	47	theme	limited	127:133	arg1	stability					135:143	The limited stability	123:143	The limited stability of catanionic vesicles	123:166	The limited stability of catanionic vesicles has discouraged their wide use for encapsulation and controlled release of active substances.					
29990535	6	48	theme	Molar	875:879	arg1	ratio					881:885	Molar ratio	875:885	Molar ratio	875:885	Molar ratio and sample preparation procedures have been investigated to evaluate the temperature stability of catanionic vesicles.					
29990535	5	49	theme	catanionic	854:863	arg1	vesicles					865:872	SDS/CTAB catanionic vesicles	845:872	SDS/CTAB catanionic vesicles	845:872	This study focuses on the impact of β-cyclodextrins for the stabilization of SDS/CTAB catanionic vesicles.					
29990535	0	50	dep	Stabilization	0:12	arg1	strategy					79:86	A strategy	77:86	Stabilization of unilamellar catanionic vesicles induced by β-cyclodextrins: A strategy for a tunable drug delivery depot.	0:121	Stabilization of unilamellar catanionic vesicles induced by β-cyclodextrins: A strategy for a tunable drug delivery depot.					
29990535	3	51	theme	hydrophobic	574:584	arg1	molecules					602:610	both hydrophobic and hydrophilic molecules	569:610	both hydrophobic and hydrophilic molecules	569:610	However, despite the limited stability, catanionic vesicles possess an attractive architecture, which is able to efficiently encapsulate both hydrophobic and hydrophilic molecules.					
29990535	1	52	theme	active	243:248	arg1	substances					250:259	active substances	243:259	active substances	243:259	The limited stability of catanionic vesicles has discouraged their wide use for encapsulation and controlled release of active substances.					
30798154	4	0	theme	P < .05	619:625	arg1	oxidation					634:642	(P < .05) lipid oxidation	618:642	(P < .05) lipid oxidation	618:642	The coatings effectively retarded (P < .05) lipid oxidation and microbial growth (psychrotrophic bacteria, molds, and yeast), extending the shelf-life from 6 to 10 days.					
30798154	4	1	theme	lipid	628:632	arg1	oxidation					634:642	(P < .05) lipid oxidation	618:642	(P < .05) lipid oxidation	618:642	The coatings effectively retarded (P < .05) lipid oxidation and microbial growth (psychrotrophic bacteria, molds, and yeast), extending the shelf-life from 6 to 10 days.					
30798154	0	2	theme	chitosan-gelatin	64:79	arg1	composites					81:90	bilayer chitosan-gelatin composites	56:90	bilayer chitosan-gelatin composites	56:90	Retail display of beef steaks coated with monolayer and bilayer chitosan-gelatin composites.					
30798154	3	3	theme	coated	571:576	arg1	ones					578:581	the coated ones	567:581	the coated ones	567:581	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	3	4	theme	control	494:500	arg1	samples					502:508	the control samples	490:508	the control samples	490:508	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	4	5	theme	psychrotrophic	666:679	arg1	bacteria					681:688	psychrotrophic bacteria	666:688	psychrotrophic bacteria	666:688	The coatings effectively retarded (P < .05) lipid oxidation and microbial growth (psychrotrophic bacteria, molds, and yeast), extending the shelf-life from 6 to 10 days.					
30798154	6	6	theme	beef	1113:1116	arg1	shelf-life					1082:1091	shelf-life	1082:1091	shelf-life	1082:1091	These effects were similar for both the monolayer and bilayer films, suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage.					
30798154	6	6	theme	beef	1113:1116	arg1	display-life					1097:1108	display-life	1097:1108	display-life	1097:1108	These effects were similar for both the monolayer and bilayer films, suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage.					
30798154	5	7	theme	CIE	882:884	arg1	indices					892:898	CIE color indices	882:898	CIE color indices	882:898	The coatings also significantly prevented metmyoglobin accumulation on the beef surface, as reflected in the maintenance of the CIE color indices and sensorial redness perception throughout storage.					
30798154	1	8	theme	beef	253:256	arg1	steaks					258:263	beef steaks	253:263	beef steaks	253:263	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	5	9	theme	color	886:890	arg1	indices					892:898	CIE color indices	882:898	CIE color indices	882:898	The coatings also significantly prevented metmyoglobin accumulation on the beef surface, as reflected in the maintenance of the CIE color indices and sensorial redness perception throughout storage.					
30798154	1	10	theme	weight	170:175	arg1	loss					177:180	weight loss	170:180	weight loss	170:180	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	5	11	theme	beef	829:832	arg1	surface					834:840	the beef surface	825:840	the beef surface	825:840	The coatings also significantly prevented metmyoglobin accumulation on the beef surface, as reflected in the maintenance of the CIE color indices and sensorial redness perception throughout storage.					
30798154	5	12	theme	indices	892:898	arg1	maintenance					863:873	the maintenance	859:873	the maintenance of the CIE color indices and sensorial redness perception throughout storage	859:950	The coatings also significantly prevented metmyoglobin accumulation on the beef surface, as reflected in the maintenance of the CIE color indices and sensorial redness perception throughout storage.					
30798154	6	13	theme	bilayer	1007:1013	arg1	films					1015:1019	both the monolayer and bilayer films	984:1019	films	1015:1019	These effects were similar for both the monolayer and bilayer films, suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage.					
30798154	6	14	theme	monolayer	993:1001	arg1	films					1015:1019	both the monolayer and bilayer films	984:1019	films	1015:1019	These effects were similar for both the monolayer and bilayer films, suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage.					
30798154	2	15	theme	bilayer	407:413	arg1	films					415:419	the bilayer films	403:419	the bilayer films	403:419	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	2	15	theme	bilayer	407:413	arg1	times					431:435	two times	427:435	two times thicker than the monolayer coatings	427:471	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	2	16	theme	thicker	437:443	arg1	films					415:419	the bilayer films	403:419	the bilayer films	403:419	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	2	16	theme	thicker	437:443	arg1	times					431:435	two times	427:435	two times thicker than the monolayer coatings	427:471	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	2	17	theme	monolayer	454:462	arg1	coatings					464:471	the monolayer coatings	450:471	the monolayer coatings	450:471	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	3	18	theme	pH	521:522	arg1	values					524:529	higher pH values	514:529	higher pH values	514:529	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	2	19	theme	edible	344:349	arg1	coatings					351:358	The edible coatings	340:358	The edible coatings	340:358	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	0	20	theme	Retail	0:5	arg1	display					7:13	Retail display	0:13	Retail display of beef steaks	0:28	Retail display of beef steaks coated with monolayer and bilayer chitosan-gelatin composites.					
30798154	1	21	theme	steaks	258:263	arg1	shelf-life					239:248	shelf-life	239:248	shelf-life	239:248	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	21	theme	steaks	258:263	arg1	display-life					220:231	display-life	220:231	display-life	220:231	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	21	theme	steaks	258:263	arg1	loss					177:180	weight loss	170:180	weight loss	170:180	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	21	theme	steaks	258:263	arg1	oxidation					189:197	lipid oxidation	183:197	lipid oxidation	183:197	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	21	theme	steaks	258:263	arg1	preservation					206:217	color preservation	200:217	color preservation (display-life)	200:232	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	4	22	theme	microbial	648:656	arg1	growth					658:663	microbial growth	648:663	microbial growth (psychrotrophic bacteria, molds, and yeast)	648:707	The coatings effectively retarded (P < .05) lipid oxidation and microbial growth (psychrotrophic bacteria, molds, and yeast), extending the shelf-life from 6 to 10 days.					
30798154	1	23	theme	lipid	183:187	arg1	oxidation					189:197	lipid oxidation	183:197	lipid oxidation	183:197	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	0	24	theme	beef	18:21	arg1	steaks					23:28	beef steaks	18:28	beef steaks	18:28	Retail display of beef steaks coated with monolayer and bilayer chitosan-gelatin composites.					
30798154	3	25	contain	had	510:512	arg2	values					524:529	higher pH values	514:529	higher pH values	514:529	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	3	25	contain	had	510:512	arg2	P < .05					553:559	P < .05	553:559	P < .05	553:559	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	3	25	contain	had	510:512	arg1	samples					502:508	the control samples	490:508	the control samples	490:508	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	3	25	contain	had	510:512	arg2	loss					547:550	more weight loss	535:550	more weight loss (P < .05)	535:560	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	3	26	theme	more	535:538	arg1	P < .05					553:559	P < .05	553:559	P < .05	553:559	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	3	26	theme	more	535:538	arg1	loss					547:550	more weight loss	535:550	more weight loss (P < .05)	535:560	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	6	27	dep	films	1015:1019	arg1	both					984:987	both	984:987	both	984:987	These effects were similar for both the monolayer and bilayer films, suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage.					
30798154	3	28	theme	higher	514:519	arg1	values					524:529	higher pH values	514:529	higher pH values	514:529	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	2	29	theme	crack-free	378:387	arg1	surface					389:395	a homogeneous crack-free surface	364:395	a homogeneous crack-free surface	364:395	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	5	30	dep	indices	892:898	arg1	the					878:880	the	878:880	the	878:880	The coatings also significantly prevented metmyoglobin accumulation on the beef surface, as reflected in the maintenance of the CIE color indices and sensorial redness perception throughout storage.					
30798154	1	31	theme	color	200:204	arg1	display-life					220:231	display-life	220:231	display-life	220:231	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	31	theme	color	200:204	arg1	preservation					206:217	color preservation	200:217	color preservation (display-life)	200:232	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	3	32	theme	weight	540:545	arg1	P < .05					553:559	P < .05	553:559	P < .05	553:559	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	3	32	theme	weight	540:545	arg1	loss					547:550	more weight loss	535:550	more weight loss (P < .05)	535:560	During storage, the control samples had higher pH values and more weight loss (P < .05) than the coated ones.					
30798154	5	33	theme	metmyoglobin	796:807	arg1	accumulation					809:820	metmyoglobin accumulation	796:820	metmyoglobin accumulation	796:820	The coatings also significantly prevented metmyoglobin accumulation on the beef surface, as reflected in the maintenance of the CIE color indices and sensorial redness perception throughout storage.					
30798154	2	34	theme	homogeneous	366:376	arg1	surface					389:395	a homogeneous crack-free surface	364:395	a homogeneous crack-free surface	364:395	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	0	35	theme	steaks	23:28	arg1	display					7:13	Retail display	0:13	Retail display of beef steaks	0:28	Retail display of beef steaks coated with monolayer and bilayer chitosan-gelatin composites.					
30798154	1	36	theme	retail	283:288	arg1	display					290:296	retail display	283:296	retail display (at 4 °C; 12 h light/day)	283:322	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	36	theme	retail	283:288	arg1	light/day					313:321	at 4 °C; 12 h light/day	299:321	at 4 °C; 12 h light/day	299:321	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	6	37	dep	shelf-life	1082:1091	arg1	the					1078:1080	the	1078:1080	the	1078:1080	These effects were similar for both the monolayer and bilayer films, suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage.					
30798154	1	38	theme	display	290:296	arg1	10 days					272:278	10 days	272:278	10 days of retail display (at 4 °C; 12 h light/day)	272:322	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	2	39	contain	had	360:362	arg1	coatings					351:358	The edible coatings	340:358	The edible coatings	340:358	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	2	39	contain	had	360:362	arg2	surface					389:395	a homogeneous crack-free surface	364:395	a homogeneous crack-free surface	364:395	The edible coatings had a homogeneous crack-free surface, with the bilayer films being two times thicker than the monolayer coatings.					
30798154	4	40	from	6	740:740	arg1	shelf-life					724:733	the shelf-life	720:733	the shelf-life from 6 to 10 days	720:751	The coatings effectively retarded (P < .05) lipid oxidation and microbial growth (psychrotrophic bacteria, molds, and yeast), extending the shelf-life from 6 to 10 days.					
30798154	1	41	theme	edible	108:113	arg1	monolayer					115:123	edible monolayer	108:123	edible monolayer	108:123	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	42	dep	loss	177:180	arg1	the					166:168	the	166:168	the	166:168	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	43	theme	monolayer	115:123	arg1	effects					97:103	The effects	93:103	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day)	93:322	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	44	from	effects	97:103	arg1	shelf-life					239:248	shelf-life	239:248	shelf-life	239:248	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	44	from	effects	97:103	arg1	display-life					220:231	display-life	220:231	display-life	220:231	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	44	from	effects	97:103	arg1	loss					177:180	weight loss	170:180	weight loss	170:180	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	44	from	effects	97:103	arg1	oxidation					189:197	lipid oxidation	183:197	lipid oxidation	183:197	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	1	44	from	effects	97:103	arg1	preservation					206:217	color preservation	200:217	color preservation (display-life)	200:232	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	6	45	theme	retail	1125:1130	arg1	storage					1132:1138	retail storage	1125:1138	retail storage	1125:1138	These effects were similar for both the monolayer and bilayer films, suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage.					
30798154	4	46	dep	growth	658:663	arg1	bacteria					681:688	psychrotrophic bacteria	666:688	psychrotrophic bacteria	666:688	The coatings effectively retarded (P < .05) lipid oxidation and microbial growth (psychrotrophic bacteria, molds, and yeast), extending the shelf-life from 6 to 10 days.					
30798154	4	46	dep	growth	658:663	arg1	molds					691:695	molds	691:695	molds	691:695	The coatings effectively retarded (P < .05) lipid oxidation and microbial growth (psychrotrophic bacteria, molds, and yeast), extending the shelf-life from 6 to 10 days.					
30798154	4	46	dep	growth	658:663	arg1	yeast					702:706	yeast	702:706	yeast	702:706	The coatings effectively retarded (P < .05) lipid oxidation and microbial growth (psychrotrophic bacteria, molds, and yeast), extending the shelf-life from 6 to 10 days.					
30798154	6	47	theme	chitosan-gelatin	1038:1053	arg1	coatings					1055:1062	chitosan-gelatin coatings	1038:1062	chitosan-gelatin coatings	1038:1062	These effects were similar for both the monolayer and bilayer films, suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage.					
30798154	6	48	dep	similar	972:978	arg1	suggesting					1022:1031	suggesting	1022:1031	suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage	1022:1138	These effects were similar for both the monolayer and bilayer films, suggesting that chitosan-gelatin coatings could improve the shelf-life and display-life of beef during retail storage.					
30798154	5	49	theme	sensorial	904:912	arg1	perception					922:931	sensorial redness perception	904:931	sensorial redness perception	904:931	The coatings also significantly prevented metmyoglobin accumulation on the beef surface, as reflected in the maintenance of the CIE color indices and sensorial redness perception throughout storage.					
30798154	1	50	theme	bilayer	129:135	arg1	coatings					154:161	bilayer chitosan-gelatin coatings	129:161	bilayer chitosan-gelatin coatings	129:161	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	0	51	theme	bilayer	56:62	arg1	composites					81:90	bilayer chitosan-gelatin composites	56:90	bilayer chitosan-gelatin composites	56:90	Retail display of beef steaks coated with monolayer and bilayer chitosan-gelatin composites.					
30798154	5	52	theme	redness	914:920	arg1	perception					922:931	sensorial redness perception	904:931	sensorial redness perception	904:931	The coatings also significantly prevented metmyoglobin accumulation on the beef surface, as reflected in the maintenance of the CIE color indices and sensorial redness perception throughout storage.					
30798154	1	53	theme	chitosan-gelatin	137:152	arg1	coatings					154:161	bilayer chitosan-gelatin coatings	129:161	bilayer chitosan-gelatin coatings	129:161	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30798154	5	54	theme	perception	922:931	arg1	maintenance					863:873	the maintenance	859:873	the maintenance of the CIE color indices and sensorial redness perception throughout storage	859:950	The coatings also significantly prevented metmyoglobin accumulation on the beef surface, as reflected in the maintenance of the CIE color indices and sensorial redness perception throughout storage.					
30798154	1	55	theme	coatings	154:161	arg1	effects					97:103	The effects	93:103	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day)	93:322	The effects of edible monolayer and bilayer chitosan-gelatin coatings on the weight loss, lipid oxidation, color preservation (display-life), and shelf-life of beef steaks during 10 days of retail display (at 4 °C; 12 h light/day) were evaluated.					
30041609	7	0	theme	expression	2262:2271	arg1	genes					2273:2277	The specific and differential expression genes	2232:2277	The specific and differential expression genes revealed in this study	2232:2300	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	3	1	theme	present	674:680	arg1	study					682:686	the present study	670:686	the present study	670:686	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	6	2	theme	Suc	2041:2043	arg1	pathways					2055:2062	the Suc metabolic pathways	2037:2062	the Suc metabolic pathways in C. esculentus tubers	2037:2086	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	4	3	theme	storage	1166:1172	arg1	tissues					1174:1180	other plant storage tissues	1154:1180	other plant storage tissues	1154:1180	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	5	4	theme	SUS1	1470:1473	arg1	isoforms					1507:1514	the main SUS isoforms	1494:1514	the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway	1494:1990	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	4	theme	SUS1	1470:1473	arg1	orthologs					1457:1465	The orthologs	1453:1465	The orthologs of SUS1, SUS3 and SUS4	1453:1488	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	5	theme	sugar	1609:1613	arg1	composition					1615:1625	sugar composition	1609:1625	sugar composition	1609:1625	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	1	6	theme	high	260:263	arg1	yield					271:275	high tuber yield	260:275	high tuber yield	260:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	5	7	theme	SUS3	1476:1479	arg1	isoforms					1507:1514	the main SUS isoforms	1494:1514	the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway	1494:1990	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	7	theme	SUS3	1476:1479	arg1	orthologs					1457:1465	The orthologs	1453:1465	The orthologs of SUS1, SUS3 and SUS4	1453:1488	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	3	8	theme	esculentus	734:743	arg1	tuber					749:753	C. esculentus oil tuber	731:753	C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues	731:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	6	9	theme	distinct	2124:2131	arg1	expressions					2133:2143	several conserved and distinct expressions	2102:2143	several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue	2102:2229	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	5	10	theme	hexose	1643:1648	arg1	phosphorylation					1650:1664	(iv) cytosolic hexose phosphorylation	1628:1664	(iv) cytosolic hexose phosphorylation	1628:1664	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	1	11	theme	oil	197:199	arg1	starch					202:207	starch	202:207	starch	202:207	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	11	theme	oil	197:199	arg1	oil					197:199	oil	197:199	oil	197:199	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	11	theme	oil	197:199	arg1	amounts					186:192	significant amounts	174:192	significant amounts of oil, starch and sugar	174:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	11	theme	oil	197:199	arg1	reserves					236:243	major storage reserves	222:243	major storage reserves in tubers with high tuber yield	222:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	11	theme	oil	197:199	arg1	sugar					213:217	sugar	213:217	sugar	213:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	6	12	theme	conserved	2110:2118	arg1	expressions					2133:2143	several conserved and distinct expressions	2102:2143	several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue	2102:2229	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	2	13	theme	different	516:524	arg1	reserves					534:541	different storage reserves	516:541	different storage reserves	516:541	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	5	14	theme	Suc	1967:1969	arg1	pathway					1984:1990	Suc biosynthesis pathway	1967:1990	Suc biosynthesis pathway	1967:1990	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	3	15	theme	transcriptome	705:717	arg1	analysis					719:726	a comprehensive transcriptome analysis	689:726	a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues	689:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	1	16	theme	starch	202:207	arg1	starch					202:207	starch	202:207	starch	202:207	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	16	theme	starch	202:207	arg1	oil					197:199	oil	197:199	oil	197:199	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	16	theme	starch	202:207	arg1	amounts					186:192	significant amounts	174:192	significant amounts of oil, starch and sugar	174:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	16	theme	starch	202:207	arg1	reserves					236:243	major storage reserves	222:243	major storage reserves in tubers with high tuber yield	222:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	16	theme	starch	202:207	arg1	sugar					213:217	sugar	213:217	sugar	213:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	7	17	theme	specific	2236:2243	arg1	genes					2273:2277	The specific and differential expression genes	2232:2277	The specific and differential expression genes revealed in this study	2232:2300	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	5	18	theme	SUS	1503:1505	arg1	isoforms					1507:1514	the main SUS isoforms	1494:1514	the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway	1494:1990	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	18	theme	SUS	1503:1505	arg1	orthologs					1457:1465	The orthologs	1453:1465	The orthologs of SUS1, SUS3 and SUS4	1453:1488	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	3	19	theme	tuber	749:753	arg1	analysis					719:726	a comprehensive transcriptome analysis	689:726	a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues	689:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	1	20	theme	underground	381:391	arg1	roots					425:429	roots	425:429	roots	425:429	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	20	theme	underground	381:391	arg1	tissues					398:404	underground sink tissues	381:404	underground sink tissues such as tubers and roots	381:429	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	20	theme	underground	381:391	arg1	tubers					414:419	tubers	414:419	tubers	414:419	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	5	21	theme	glucose	1736:1742	arg1	UGP					1763:1765	UGP	1763:1765	UGP	1763:1765	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	21	theme	glucose	1736:1742	arg1	pyrophosphorylase					1744:1760	uridine diphosphate glucose pyrophosphorylase	1716:1760	uridine diphosphate glucose pyrophosphorylase (UGP)	1716:1766	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	1	22	theme	sugar	213:217	arg1	starch					202:207	starch	202:207	starch	202:207	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	22	theme	sugar	213:217	arg1	oil					197:199	oil	197:199	oil	197:199	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	22	theme	sugar	213:217	arg1	amounts					186:192	significant amounts	174:192	significant amounts of oil, starch and sugar	174:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	22	theme	sugar	213:217	arg1	reserves					236:243	major storage reserves	222:243	major storage reserves in tubers with high tuber yield	222:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	22	theme	sugar	213:217	arg1	sugar					213:217	sugar	213:217	sugar	213:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	4	23	theme	dissimilar	1140:1149	arg1	pattern					1132:1138	a pattern	1130:1138	a pattern dissimilar to other plant storage tissues	1130:1180	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	5	24	theme	SPS	1843:1845	arg1	B-					1848:1849	Suc-phosphate synthase (SPS) B- and C-family members	1819:1870	B-	1848:1849	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	1	25	from	reserves	236:243	arg1	tubers					248:253	tubers	248:253	tubers with high tuber yield	248:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	6	26	from	accumulation	2176:2187	arg1	tissue					2224:2229	this unique underground storage tissue	2192:2229	this unique underground storage tissue	2192:2229	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	2	27	theme	reserves	534:541	arg1	biosynthesis					500:511	biosynthesis	500:511	biosynthesis of different storage reserves	500:541	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	4	28	theme	other	1154:1158	arg1	tissues					1174:1180	other plant storage tissues	1154:1180	other plant storage tissues	1154:1180	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	3	29	theme	plant	773:777	arg1	tissues					813:819	other plant oil- or carbohydrate-rich storage tissues	767:819	other plant oil- or carbohydrate-rich storage tissues	767:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	4	30	dep	regulated	1106:1114	arg1	both					1188:1191	both	1188:1191	both	1188:1191	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	30	dep	regulated	1106:1114	arg1	ii					1184:1185	ii	1184:1185	ii	1184:1185	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	6	31	theme	sugar	2170:2174	arg1	accumulation					2176:2187	sugar accumulation	2170:2187	sugar accumulation in this unique underground storage tissue	2170:2229	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	4	32	theme	Suc	1381:1383	arg1	SUS					1395:1397	SUS	1395:1397	SUS	1395:1397	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	32	theme	Suc	1381:1383	arg1	synthase					1385:1392	Suc synthase	1381:1392	Suc synthase (SUS) other than invertase (INV)	1381:1425	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	0	33	theme	esculentus	60:69	arg1	tubers					42:47	oil-rich tubers	33:47	oil-rich tubers of Cyperus esculentus	33:69	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis.					
30041609	4	34	theme	transcripts	966:976	arg1	features					949:956	some species-specific features	927:956	some species-specific features of gene transcripts	927:976	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	5	35	theme	uridine	1716:1722	arg1	UGP					1763:1765	UGP	1763:1765	UGP	1763:1765	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	35	theme	uridine	1716:1722	arg1	pyrophosphorylase					1744:1760	uridine diphosphate glucose pyrophosphorylase	1716:1760	uridine diphosphate glucose pyrophosphorylase (UGP)	1716:1766	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	2	36	theme	central	454:460	arg1	role					462:465	a central role	452:465	a central role	452:465	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	3	37	theme	storage	805:811	arg1	tissues					813:819	other plant oil- or carbohydrate-rich storage tissues	767:819	other plant oil- or carbohydrate-rich storage tissues	767:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	0	38	theme	comparative	72:82	arg1	analysis					98:105	comparative transcriptome analysis	72:105	comparative transcriptome analysis	72:105	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis.					
30041609	3	39	theme	expression	838:847	arg1	patterns					849:856	the expression patterns	834:856	the expression patterns of genes related to the Suc metabolism	834:895	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	2	40	theme	flux	488:491	arg1	control					470:476	control	470:476	control of carbon flux toward biosynthesis of different storage reserves	470:541	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	1	41	dep	unique	141:146	arg1	considered					291:300	considered	291:300	considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots	291:429	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	4	42	dep	RESULTS	898:904	arg1	results					910:916	The results	906:916	RESULTS The results	898:916	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	43	theme	species-specific	932:947	arg1	features					949:956	some species-specific features	927:956	some species-specific features of gene transcripts	927:976	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	7	44	theme	metabolism	2387:2396	arg1	regulation					2369:2378	transcriptional regulation	2353:2378	transcriptional regulation of Suc metabolism	2353:2396	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	1	45	theme	different	351:359	arg1	reserves					369:376	different storage reserves	351:376	different storage reserves	351:376	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	0	46	dep	analysis	98:105	arg1	metabolism					8:17	Sucrose metabolism	0:17	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus	0:69	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis.					
30041609	3	47	theme	related	867:873	arg1	genes					861:865	genes	861:865	genes related to the Suc metabolism	861:895	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	4	48	theme	esculentus	997:1006	arg1	tuber					985:989	oil tuber	981:989	oil tuber of C. esculentus	981:1006	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	2	49	theme	storage	650:656	arg1	tissues					658:664	underground oil-rich storage tissues	629:664	underground oil-rich storage tissues	629:664	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	4	50	theme	major	1250:1254	arg1	expressions					1034:1044	expressions	1034:1044	expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown	1034:1208	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	50	theme	major	1250:1254	arg1	pathways					1256:1263	the major pathways	1246:1263	the major pathways	1246:1263	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	50	theme	major	1250:1254	arg1	processes					1227:1235	biosynthesis processes	1214:1235	biosynthesis processes	1214:1235	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	0	51	theme	Sucrose	0:6	arg1	metabolism					8:17	Sucrose metabolism	0:17	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus	0:69	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis.					
30041609	1	52	theme	tuber	265:269	arg1	yield					271:275	high tuber yield	260:275	high tuber yield	260:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	6	53	theme	storage	2216:2222	arg1	tissue					2224:2229	this unique underground storage tissue	2192:2229	this unique underground storage tissue	2192:2229	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	7	54	theme	transcriptional	2353:2367	arg1	regulation					2369:2378	transcriptional regulation	2353:2378	transcriptional regulation of Suc metabolism	2353:2396	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	2	55	theme	underground	629:639	arg1	tissues					658:664	underground oil-rich storage tissues	629:664	underground oil-rich storage tissues	629:664	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	5	56	theme	vacuolar	1551:1558	arg1	determinant					1585:1595	the leading determinant	1573:1595	the leading determinant controlling sugar composition	1573:1625	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	56	theme	vacuolar	1551:1558	arg1	VIN					1565:1567	VIN	1565:1567	VIN	1565:1567	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	56	theme	vacuolar	1551:1558	arg1	INV					1560:1562	the vacuolar INV	1547:1562	the vacuolar INV (VIN)	1547:1568	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	6	57	theme	unique	2197:2202	arg1	tissue					2224:2229	this unique underground storage tissue	2192:2229	this unique underground storage tissue	2192:2229	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	4	58	theme	oil	981:983	arg1	tuber					985:989	oil tuber	981:989	oil tuber of C. esculentus	981:1006	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	0	59	theme	oil-rich	33:40	arg1	tubers					42:47	oil-rich tubers	33:47	oil-rich tubers of Cyperus esculentus	33:69	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis.					
30041609	7	60	theme	special	2321:2327	arg1	mechanism					2339:2347	the special molecular mechanism	2317:2347	the special molecular mechanism	2317:2347	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	5	61	dep	SPS	1884:1886	arg1	contributors					1913:1924	the principal contributors	1899:1924	the principal contributors to SPS enzymes	1899:1939	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	61	dep	SPS	1884:1886	arg1	A					1888:1888	A	1888:1888	A	1888:1888	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	4	62	theme	Suc	1196:1198	arg1	breakdown					1200:1208	Suc breakdown	1196:1208	Suc breakdown	1196:1208	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	5	63	theme	crucial	1950:1956	arg1	roles					1958:1962	crucial roles	1950:1962	crucial roles	1950:1962	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	1	64	theme	storage	228:234	arg1	starch					202:207	starch	202:207	starch	202:207	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	64	theme	storage	228:234	arg1	reserves					236:243	major storage reserves	222:243	major storage reserves in tubers with high tuber yield	222:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	64	theme	storage	228:234	arg1	oil					197:199	oil	197:199	oil	197:199	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	64	theme	storage	228:234	arg1	amounts					186:192	significant amounts	174:192	significant amounts of oil, starch and sugar	174:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	64	theme	storage	228:234	arg1	sugar					213:217	sugar	213:217	sugar	213:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	4	65	dep	was	1306:1308	arg1	iii					1298:1300	iii	1298:1300	iii	1298:1300	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	5	66	theme	principal	1903:1911	arg1	contributors					1913:1924	the principal contributors	1899:1924	the principal contributors to SPS enzymes	1899:1939	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	66	theme	principal	1903:1911	arg1	A					1888:1888	A	1888:1888	A	1888:1888	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	7	67	theme	esculentus	2427:2436	arg1	tubers					2414:2419	oil tubers	2410:2419	oil tubers of C. esculentus	2410:2436	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	4	68	theme	Suc	1281:1283	arg1	metabolism					1285:1294	Suc metabolism	1281:1294	Suc metabolism	1281:1294	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	69	theme	tuber	1434:1438	arg1	development					1440:1450	tuber development	1434:1450	tuber development	1434:1450	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	6	70	theme	metabolic	2045:2053	arg1	pathways					2055:2062	the Suc metabolic pathways	2037:2062	the Suc metabolic pathways in C. esculentus tubers	2037:2086	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	5	71	dep	B-	1848:1849	arg1	v					1810:1810	v	1810:1810	v	1810:1810	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	72	theme	SPS	1929:1931	arg1	enzymes					1933:1939	SPS enzymes	1929:1939	SPS enzymes	1929:1939	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	73	theme	Suc-phosphate	1819:1831	arg1	B-					1848:1849	Suc-phosphate synthase (SPS) B- and C-family members	1819:1870	B-	1848:1849	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	7	74	theme	oil	2410:2412	arg1	tubers					2414:2419	oil tubers	2410:2419	oil tubers of C. esculentus	2410:2436	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	7	75	theme	differential	2249:2260	arg1	genes					2273:2277	The specific and differential expression genes	2232:2277	The specific and differential expression genes revealed in this study	2232:2300	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	3	76	theme	comprehensive	691:703	arg1	analysis					719:726	a comprehensive transcriptome analysis	689:726	a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues	689:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	1	77	theme	significant	174:184	arg1	starch					202:207	starch	202:207	starch	202:207	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	77	theme	significant	174:184	arg1	oil					197:199	oil	197:199	oil	197:199	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	77	theme	significant	174:184	arg1	amounts					186:192	significant amounts	174:192	significant amounts of oil, starch and sugar	174:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	77	theme	significant	174:184	arg1	reserves					236:243	major storage reserves	222:243	major storage reserves in tubers with high tuber yield	222:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	77	theme	significant	174:184	arg1	sugar					213:217	sugar	213:217	sugar	213:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	2	78	theme	molecular	580:588	arg1	mechanism					590:598	the molecular mechanism	576:598	the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues	576:664	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	5	79	theme	Suc	1527:1529	arg1	breakdown					1531:1539	Suc breakdown	1527:1539	Suc breakdown	1527:1539	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	80	theme	cytosolic	1633:1641	arg1	phosphorylation					1650:1664	(iv) cytosolic hexose phosphorylation	1628:1664	(iv) cytosolic hexose phosphorylation	1628:1664	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	4	81	theme	responsible	1055:1065	arg1	genes					1049:1053	genes	1049:1053	genes responsible for Suc metabolism	1049:1084	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	3	82	theme	C.	731:732	arg1	tuber					749:753	C. esculentus oil tuber	731:753	C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues	731:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	6	83	theme	several	2102:2108	arg1	expressions					2133:2143	several conserved and distinct expressions	2102:2143	several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue	2102:2229	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	4	84	dep	pathways	1256:1263	arg1	associated					1265:1274	associated	1265:1274	associated with Suc metabolism	1265:1294	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	85	theme	Suc	1071:1073	arg1	metabolism					1075:1084	Suc metabolism	1071:1084	Suc metabolism	1071:1084	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	7	86	theme	Suc	2383:2385	arg1	metabolism					2387:2396	Suc metabolism	2383:2396	Suc metabolism	2383:2396	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	5	87	theme	main	1498:1501	arg1	isoforms					1507:1514	the main SUS isoforms	1494:1514	the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway	1494:1990	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	87	theme	main	1498:1501	arg1	orthologs					1457:1465	The orthologs	1453:1465	The orthologs of SUS1, SUS3 and SUS4	1453:1488	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	4	88	theme	Suc	1324:1326	arg1	degradation					1328:1338	Suc degradation	1324:1338	Suc degradation	1324:1338	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	5	89	theme	diphosphate	1724:1734	arg1	UGP					1763:1765	UGP	1763:1765	UGP	1763:1765	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	89	theme	diphosphate	1724:1734	arg1	pyrophosphorylase					1744:1760	uridine diphosphate glucose pyrophosphorylase	1716:1760	uridine diphosphate glucose pyrophosphorylase (UGP)	1716:1766	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	1	90	theme	sink	393:396	arg1	roots					425:429	roots	425:429	roots	425:429	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	90	theme	sink	393:396	arg1	tissues					398:404	underground sink tissues	381:404	underground sink tissues such as tubers and roots	381:429	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	90	theme	sink	393:396	arg1	tubers					414:419	tubers	414:419	tubers	414:419	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	6	91	theme	esculentus	2070:2079	arg1	tubers					2081:2086	C. esculentus tubers	2067:2086	C. esculentus tubers	2067:2086	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	4	92	theme	plant	1160:1164	arg1	tissues					1174:1180	other plant storage tissues	1154:1180	other plant storage tissues	1154:1180	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	3	93	theme	oil	745:747	arg1	tuber					749:753	C. esculentus oil tuber	731:753	C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues	731:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	2	94	theme	storage	526:532	arg1	reserves					534:541	different storage reserves	516:541	different storage reserves	516:541	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	2	95	from	metabolism	615:624	arg1	tissues					658:664	underground oil-rich storage tissues	629:664	underground oil-rich storage tissues	629:664	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	3	96	theme	oil-	779:782	arg1	tissues					813:819	other plant oil- or carbohydrate-rich storage tissues	767:819	other plant oil- or carbohydrate-rich storage tissues	767:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	3	97	theme	other	767:771	arg1	tissues					813:819	other plant oil- or carbohydrate-rich storage tissues	767:819	other plant oil- or carbohydrate-rich storage tissues	767:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	0	98	theme	transcriptome	84:96	arg1	analysis					98:105	comparative transcriptome analysis	72:105	comparative transcriptome analysis	72:105	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis.					
30041609	5	99	theme	C-family	1855:1862	arg1	members					1864:1870	Suc-phosphate synthase (SPS) B- and C-family members	1819:1870	members	1864:1870	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	3	100	theme	genes	861:865	arg1	patterns					849:856	the expression patterns	834:856	the expression patterns of genes related to the Suc metabolism	834:895	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	4	101	theme	gene	961:964	arg1	transcripts					966:976	gene transcripts	961:976	gene transcripts	961:976	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	1	102	theme	carbon	328:333	arg1	allocation					335:344	carbon allocation	328:344	carbon allocation into different storage reserves	328:376	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	2	103	theme	carbon	481:486	arg1	flux					488:491	carbon flux	481:491	carbon flux	481:491	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	2	104	theme	Suc	611:613	arg1	metabolism					615:624	Suc metabolism	611:624	Suc metabolism in underground oil-rich storage tissues	611:664	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	5	105	theme	biosynthesis	1971:1982	arg1	pathway					1984:1990	Suc biosynthesis pathway	1967:1990	Suc biosynthesis pathway	1967:1990	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	3	106	theme	carbohydrate-rich	787:803	arg1	tissues					813:819	other plant oil- or carbohydrate-rich storage tissues	767:819	other plant oil- or carbohydrate-rich storage tissues	767:819	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	3	107	theme	Suc	882:884	arg1	metabolism					886:895	the Suc metabolism	878:895	the Suc metabolism	878:895	In the present study, a comprehensive transcriptome analysis of C. esculentus oil tuber compared to other plant oil- or carbohydrate-rich storage tissues was made for the expression patterns of genes related to the Suc metabolism.					
30041609	4	108	theme	other	1400:1404	arg1	SUS					1395:1397	SUS	1395:1397	SUS	1395:1397	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	108	theme	other	1400:1404	arg1	synthase					1385:1392	Suc synthase	1381:1392	Suc synthase (SUS) other than invertase (INV)	1381:1425	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	1	109	theme	storage	361:367	arg1	reserves					369:376	different storage reserves	351:376	different storage reserves	351:376	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	4	110	dep	expressions	1034:1044	arg1	the					1030:1032	the	1030:1032	the	1030:1032	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	110	dep	expressions	1034:1044	arg1	i					1027:1027	i	1027:1027	i	1027:1027	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	2	111	theme	oil-rich	641:648	arg1	tissues					658:664	underground oil-rich storage tissues	629:664	underground oil-rich storage tissues	629:664	Sucrose (Suc) plays a central role in control of carbon flux toward biosynthesis of different storage reserves; however, it remains unclear for the molecular mechanism underlying Suc metabolism in underground oil-rich storage tissues.					
30041609	5	112	theme	leading	1577:1583	arg1	determinant					1585:1595	the leading determinant	1573:1595	the leading determinant controlling sugar composition	1573:1625	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	112	theme	leading	1577:1583	arg1	INV					1560:1562	the vacuolar INV	1547:1562	the vacuolar INV (VIN)	1547:1568	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	6	113	theme	underground	2204:2214	arg1	tissue					2224:2229	this unique underground storage tissue	2192:2229	this unique underground storage tissue	2192:2229	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	7	114	theme	molecular	2329:2337	arg1	mechanism					2339:2347	the special molecular mechanism	2317:2347	the special molecular mechanism	2317:2347	The specific and differential expression genes revealed in this study might indicate the special molecular mechanism and transcriptional regulation of Suc metabolism occurred in oil tubers of C. esculentus.					
30041609	5	115	theme	important	1777:1785	arg1	role					1787:1790	an important role	1774:1790	an important role	1774:1790	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	4	116	theme	synthase	1385:1392	arg1	action					1371:1376	the action	1367:1376	the action of Suc synthase (SUS) other than invertase (INV)	1367:1425	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	6	117	dep	CONCLUSIONS	1993:2003	arg1	identified					2026:2035	identified	2026:2035	identified the Suc metabolic pathways in C. esculentus tubers	2026:2086	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	0	118	theme	Cyperus	52:58	arg1	esculentus					60:69	Cyperus esculentus	52:69	Cyperus esculentus	52:69	Sucrose metabolism in developing oil-rich tubers of Cyperus esculentus: comparative transcriptome analysis.					
30041609	5	119	theme	synthase	1833:1840	arg1	B-					1848:1849	Suc-phosphate synthase (SPS) B- and C-family members	1819:1870	B-	1848:1849	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	1	120	theme	novel	307:311	arg1	model					313:317	a novel model	305:317	a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots	305:429	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	121	with	tubers	248:253	arg1	yield					271:275	high tuber yield	260:275	high tuber yield	260:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	4	122	theme	biosynthesis	1214:1225	arg1	expressions					1034:1044	expressions	1034:1044	expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown	1034:1208	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	122	theme	biosynthesis	1214:1225	arg1	pathways					1256:1263	the major pathways	1246:1263	the major pathways	1246:1263	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	4	122	theme	biosynthesis	1214:1225	arg1	processes					1227:1235	biosynthesis processes	1214:1235	biosynthesis processes	1214:1235	RESULTS The results revealed some species-specific features of gene transcripts in oil tuber of C. esculentus, indicating that: (i) the expressions of genes responsible for Suc metabolism are developmentally regulated and displayed a pattern dissimilar to other plant storage tissues; (ii) both of Suc breakdown and biosynthesis processes might be the major pathways associated with Suc metabolism; (iii) it was probably that Suc degradation could be primarily through the action of Suc synthase (SUS) other than invertase (INV) during tuber development.					
30041609	1	123	theme	major	222:226	arg1	starch					202:207	starch	202:207	starch	202:207	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	123	theme	major	222:226	arg1	reserves					236:243	major storage reserves	222:243	major storage reserves in tubers with high tuber yield	222:275	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	123	theme	major	222:226	arg1	oil					197:199	oil	197:199	oil	197:199	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	123	theme	major	222:226	arg1	amounts					186:192	significant amounts	174:192	significant amounts of oil, starch and sugar	174:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	1	123	theme	major	222:226	arg1	sugar					213:217	sugar	213:217	sugar	213:217	BACKGROUND Cyperus esculentus is unique in that it can accumulate significant amounts of oil, starch and sugar as major storage reserves in tubers with high tuber yield and therefore considered as a novel model to study carbon allocation into different storage reserves in underground sink tissues such as tubers and roots.					
30041609	6	124	theme	C.	2067:2068	arg1	tubers					2081:2086	C. esculentus tubers	2067:2086	C. esculentus tubers	2067:2086	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
30041609	5	125	dep	phosphorylation	1650:1664	arg1	iv					1629:1630	iv	1629:1630	iv	1629:1630	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	126	theme	SUS4	1485:1488	arg1	isoforms					1507:1514	the main SUS isoforms	1494:1514	the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway	1494:1990	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	5	126	theme	SUS4	1485:1488	arg1	orthologs					1457:1465	The orthologs	1453:1465	The orthologs of SUS1, SUS3 and SUS4	1453:1488	The orthologs of SUS1, SUS3 and SUS4 are the main SUS isoforms catalyzing Suc breakdown while the vacuolar INV (VIN) is the leading determinant controlling sugar composition; (iv) cytosolic hexose phosphorylation possibly relies more on fructose as substrate and uridine diphosphate glucose pyrophosphorylase (UGP) plays an important role in this pathway; (v) it is Suc-phosphate synthase (SPS) B- and C-family members rather than SPS A that are the principal contributors to SPS enzymes and play crucial roles in Suc biosynthesis pathway.					
30041609	6	127	from	pathways	2055:2062	arg1	tubers					2081:2086	C. esculentus tubers	2067:2086	C. esculentus tubers	2067:2086	CONCLUSIONS We have successfully identified the Suc metabolic pathways in C. esculentus tubers, highlighting several conserved and distinct expressions that might contribute to sugar accumulation in this unique underground storage tissue.					
31575381	11	0	theme	epithelium	1795:1804	arg1	hyperplasia					1753:1763	hyperplasia	1753:1763	hyperplasia	1753:1763	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	11	0	theme	epithelium	1795:1804	arg1	resorption					1720:1729	osteoclast resorption	1709:1729	osteoclast resorption of alveolar bone	1709:1746	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	11	0	theme	epithelium	1795:1804	arg1	migration					1769:1777	migration	1769:1777	migration	1769:1777	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	11	0	theme	epithelium	1795:1804	arg1	infiltration					1655:1666	inflammatory cell infiltration	1637:1666	inflammatory cell infiltration (predominantly T cells and macrophages)	1637:1706	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	12	1	theme	noteworthy	1927:1936	arg1	abnormalities					1948:1960	any noteworthy metabolic abnormalities	1923:1960	any noteworthy metabolic abnormalities	1923:1960	HSC-fed Lewis rats developed periodontitis without mechanical intervention in the oral cavity and in the absence of any noteworthy metabolic abnormalities.					
31575381	4	2	from	casein	527:532	arg1	high					507:510	high	507:510	high	507:510	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	6	3	theme	Lewis	776:780	arg1	rats					782:785	Lewis rats	776:785	Lewis rats	776:785	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	11	4	dep	infiltration	1655:1666	arg1	macrophages					1695:1705	macrophages	1695:1705	macrophages	1695:1705	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	11	4	dep	infiltration	1655:1666	arg1	cells					1685:1689	T cells	1683:1689	T cells	1683:1689	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	12	5	theme	abnormalities	1948:1960	arg1	absence					1912:1918	the absence	1908:1918	the absence of any noteworthy metabolic abnormalities	1908:1960	HSC-fed Lewis rats developed periodontitis without mechanical intervention in the oral cavity and in the absence of any noteworthy metabolic abnormalities.					
31575381	6	6	theme	HSC	822:824	arg1	chow					835:838	standard (STD, n = 12) or HSC (n = 20) chow	796:838	chow	835:838	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	6	6	theme	HSC	822:824	arg1	n					827:827	n = 20	827:832	n = 20	827:832	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	4	7	from	sucrose	515:521	arg1	high					507:510	high	507:510	high	507:510	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	10	8	theme	bone	1343:1346	arg1	densitometry					1348:1359	bone densitometry	1343:1359	bone densitometry	1343:1359	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	4	9	from	high	507:510	arg1	sucrose					515:521	sucrose	515:521	sucrose	515:521	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	4	9	from	high	507:510	arg1	casein					527:532	casein	527:532	casein	527:532	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	11	10	theme	Lewis	1481:1485	arg1	rats					1487:1490	Lewis rats	1481:1490	Lewis rats	1481:1490	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	10	11	from	densitometry	1348:1359	arg1	liver					1466:1470	the liver	1462:1470	the liver	1462:1470	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	3	12	theme	exacerbated	301:311	arg1	response					317:324	their exacerbated Th1 response	295:324	their exacerbated Th1 response	295:324	Due to their exacerbated Th1 response and imbalanced Th17 regulatory T-cell responses, Lewis rats are highly susceptible to inducible inflammatory and autoimmune diseases.					
31575381	11	13	theme	osteoclast	1709:1718	arg1	resorption					1720:1729	osteoclast resorption	1709:1729	osteoclast resorption of alveolar bone	1709:1746	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	2	14	from	Rattus	148:153	arg1	rodents					125:131	rodents	125:131	rodents from the genus Rattus	125:153	Because rodents from the genus Rattus are resistant to spontaneous periodontitis, experimental periodontitis must be initiated by mechanical procedures and interventions.					
31575381	4	15	theme	Lewis	489:493	arg1	rats					495:498	feeding Lewis rats a diet high in sucrose and casein (HSC)	481:538	feeding Lewis rats a diet high in sucrose and casein (HSC)	481:538	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	5	16	theme	age	728:730	arg1	wk					734:735	age 6 wk	728:735	age 6 wk	728:735	A baseline group (BSL, n = 8) was euthanized at age 6 wk.					
31575381	9	17	theme	innate	1162:1167	arg1	cytokines					1178:1186	innate immunity cytokines	1162:1186	innate immunity cytokines	1162:1186	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	2	18	theme	mechanical	247:256	arg1	procedures					258:267	mechanical procedures	247:267	mechanical procedures	247:267	Because rodents from the genus Rattus are resistant to spontaneous periodontitis, experimental periodontitis must be initiated by mechanical procedures and interventions.					
31575381	7	19	theme	periodontitis	899:911	arg1	degree					889:894	the degree	885:894	the degree of periodontitis	885:911	We evaluated the degree of periodontitis through histology and μCT of maxillae and mandibles.					
31575381	9	20	theme	Gene	1071:1074	arg1	analysis					1087:1094	Gene expression analysis	1071:1094	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria	1071:1229	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	10	21	from	leptin	1378:1383	arg1	liver					1466:1470	the liver	1462:1470	the liver	1462:1470	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	3	22	theme	imbalanced	330:339	arg1	responses					364:372	imbalanced Th17 regulatory T-cell responses	330:372	imbalanced Th17 regulatory T-cell responses	330:372	Due to their exacerbated Th1 response and imbalanced Th17 regulatory T-cell responses, Lewis rats are highly susceptible to inducible inflammatory and autoimmune diseases.					
31575381	11	23	theme	bone	1743:1746	arg1	hyperplasia					1753:1763	hyperplasia	1753:1763	hyperplasia	1753:1763	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	11	23	theme	bone	1743:1746	arg1	resorption					1720:1729	osteoclast resorption	1709:1729	osteoclast resorption of alveolar bone	1709:1746	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	11	23	theme	bone	1743:1746	arg1	migration					1769:1777	migration	1769:1777	migration	1769:1777	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	11	23	theme	bone	1743:1746	arg1	infiltration					1655:1666	inflammatory cell infiltration	1637:1666	inflammatory cell infiltration (predominantly T cells and macrophages)	1637:1706	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	5	24	theme	=	705:705	arg1	n					703:703	n = 8	703:707	n = 8	703:707	A baseline group (BSL, n = 8) was euthanized at age 6 wk.					
31575381	13	25	theme	promising	2018:2026	arg1	similar					2089:2095	similar	2089:2095	similar	2089:2095	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	13	25	theme	promising	2018:2026	arg1	approach					2028:2035	a promising approach	2016:2035	a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease	2016:2132	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	13	25	theme	promising	2018:2026	arg1	model					1985:1989	the rat model	1977:1989	the rat model we described here	1977:2007	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	9	26	theme	inflammatory	1099:1110	arg1	cytokines					1112:1120	inflammatory cytokines	1099:1120	inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria	1099:1229	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	11	27	theme	T	1683:1683	arg1	cells					1685:1689	T cells	1683:1689	T cells	1683:1689	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	12	28	from	intervention	1869:1880	arg1	cavity					1894:1899	the oral cavity	1885:1899	the oral cavity	1885:1899	HSC-fed Lewis rats developed periodontitis without mechanical intervention in the oral cavity and in the absence of any noteworthy metabolic abnormalities.					
31575381	3	29	theme	regulatory	346:355	arg1	responses					364:372	imbalanced Th17 regulatory T-cell responses	330:372	imbalanced Th17 regulatory T-cell responses	330:372	Due to their exacerbated Th1 response and imbalanced Th17 regulatory T-cell responses, Lewis rats are highly susceptible to inducible inflammatory and autoimmune diseases.					
31575381	0	30	from	Periodontitis	25:37	arg1	Rats					48:51	Lewis Rats	42:51	Lewis Rats	42:51	Diet-induced Generalized Periodontitis in Lewis Rats.					
31575381	10	31	theme	serum	1372:1376	arg1	leptin					1378:1383	serum leptin and insulin concentrations	1372:1410	leptin	1378:1383	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	9	32	theme	cytokines	1112:1120	arg1	analysis					1087:1094	Gene expression analysis	1071:1094	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria	1071:1229	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	13	33	theme	rat	1981:1983	arg1	similar					2089:2095	similar	2089:2095	similar	2089:2095	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	13	33	theme	rat	1981:1983	arg1	model					1985:1989	the rat model	1977:1989	the rat model we described here	1977:2007	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	13	33	theme	rat	1981:1983	arg1	approach					2028:2035	a promising approach	2016:2035	a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease	2016:2132	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	3	34	theme	Lewis	375:379	arg1	rats					381:384	Lewis rats	375:384	Lewis rats	375:384	Due to their exacerbated Th1 response and imbalanced Th17 regulatory T-cell responses, Lewis rats are highly susceptible to inducible inflammatory and autoimmune diseases.					
31575381	4	35	theme	high	507:510	arg1	HSC					535:537	HSC	535:537	HSC	535:537	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	4	35	theme	high	507:510	arg1	diet					502:505	a diet	500:505	feeding Lewis rats a diet high in sucrose and casein (HSC)	481:538	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	12	36	theme	Lewis	1815:1819	arg1	rats					1821:1824	HSC-fed Lewis rats	1807:1824	HSC-fed Lewis rats	1807:1824	HSC-fed Lewis rats developed periodontitis without mechanical intervention in the oral cavity and in the absence of any noteworthy metabolic abnormalities.					
31575381	1	37	theme	public	84:89	arg1	worldwide					106:114	an important public health concern worldwide	71:114	an important public health concern worldwide	71:114	Periodontitis is an important public health concern worldwide.					
31575381	1	37	theme	public	84:89	arg1	Periodontitis					54:66	Periodontitis	54:66	Periodontitis	54:66	Periodontitis is an important public health concern worldwide.					
31575381	5	38	dep	BSL	698:700	arg1	n					703:703	n = 8	703:707	n = 8	703:707	A baseline group (BSL, n = 8) was euthanized at age 6 wk.					
31575381	0	39	theme	Diet-induced	0:11	arg1	Periodontitis					25:37	Diet-induced Generalized Periodontitis	0:37	Diet-induced Generalized Periodontitis in Lewis Rats	0:51	Diet-induced Generalized Periodontitis in Lewis Rats.					
31575381	1	40	theme	concern	98:104	arg1	worldwide					106:114	an important public health concern worldwide	71:114	an important public health concern worldwide	71:114	Periodontitis is an important public health concern worldwide.					
31575381	1	40	theme	concern	98:104	arg1	Periodontitis					54:66	Periodontitis	54:66	Periodontitis	54:66	Periodontitis is an important public health concern worldwide.					
31575381	13	41	from	similar	2089:2095	arg1	presentation					2100:2111	presentation	2100:2111	presentation to the human disease	2100:2132	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	0	42	theme	Lewis	42:46	arg1	Rats					48:51	Lewis Rats	42:51	Lewis Rats	42:51	Diet-induced Generalized Periodontitis in Lewis Rats.					
31575381	8	43	theme	inflammatory	982:993	arg1	response					995:1002	The HSC-induced inflammatory response	966:1002	The HSC-induced inflammatory response of periodontal tissues	966:1025	The HSC-induced inflammatory response of periodontal tissues was assessed by using immunohistochemistry.					
31575381	11	44	theme	generalized	1594:1604	arg1	periodontitis					1606:1618	generalized periodontitis	1594:1618	generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium	1594:1804	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	11	44	theme	generalized	1594:1604	arg1	tissues					1564:1570	periodontal tissues	1552:1570	periodontal tissues	1552:1570	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	10	45	theme	potential	1255:1263	arg1	effects					1274:1280	The potential systemic effects	1251:1280	The potential systemic effects of HSC diet	1251:1292	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	13	46	dep	model	1985:1989	arg1	we					1991:1992	we	1991:1992	we	1991:1992	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	10	47	theme	inflammatory	1436:1447	arg1	cytokines					1449:1457	inflammatory cytokines	1436:1457	inflammatory cytokines	1436:1457	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	8	48	theme	tissues	1019:1025	arg1	response					995:1002	The HSC-induced inflammatory response	966:1002	The HSC-induced inflammatory response of periodontal tissues	966:1025	The HSC-induced inflammatory response of periodontal tissues was assessed by using immunohistochemistry.					
31575381	4	49	theme	periodontitis	625:637	arg1	lesions					639:645	periodontitis lesions	625:645	periodontitis lesions without mechanical intervention	625:677	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	2	50	theme	experimental	199:210	arg1	periodontitis					212:224	experimental periodontitis	199:224	experimental periodontitis	199:224	Because rodents from the genus Rattus are resistant to spontaneous periodontitis, experimental periodontitis must be initiated by mechanical procedures and interventions.					
31575381	11	51	theme	Th1-immune	1529:1538	arg1	response					1540:1547	a host Th1-immune response	1522:1547	a host Th1-immune response	1522:1547	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	10	52	theme	body	1322:1325	arg1	composition					1327:1337	body composition	1322:1337	body composition	1322:1337	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	12	53	theme	oral	1889:1892	arg1	cavity					1894:1899	the oral cavity	1885:1899	the oral cavity	1885:1899	HSC-fed Lewis rats developed periodontitis without mechanical intervention in the oral cavity and in the absence of any noteworthy metabolic abnormalities.					
31575381	11	54	theme	gingival	1786:1793	arg1	epithelium					1795:1804	the gingival epithelium	1782:1804	the gingival epithelium	1782:1804	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	6	55	theme	=	829:829	arg1	chow					835:838	standard (STD, n = 12) or HSC (n = 20) chow	796:838	chow	835:838	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	6	55	theme	=	829:829	arg1	n					827:827	n = 20	827:832	n = 20	827:832	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	9	56	theme	Th1	1138:1140	arg1	responses					1151:1159	Th1 and Th17 responses	1138:1159	Th1 and Th17 responses	1138:1159	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	6	57	theme	rats	782:785	arg1	groups					766:771	2 groups	764:771	2 groups of Lewis rats	764:785	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	6	57	theme	rats	782:785	arg1	rats					782:785	Lewis rats	776:785	Lewis rats	776:785	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	4	58	dep	rats	495:498	arg1	HSC					535:537	HSC	535:537	HSC	535:537	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	4	58	dep	rats	495:498	arg1	diet					502:505	a diet	500:505	feeding Lewis rats a diet high in sucrose and casein (HSC)	481:538	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	12	59	theme	metabolic	1938:1946	arg1	abnormalities					1948:1960	any noteworthy metabolic abnormalities	1923:1960	any noteworthy metabolic abnormalities	1923:1960	HSC-fed Lewis rats developed periodontitis without mechanical intervention in the oral cavity and in the absence of any noteworthy metabolic abnormalities.					
31575381	4	60	theme	oral	556:559	arg1	microenvironment					561:576	the oral microenvironment	552:576	the oral microenvironment	552:576	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	2	61	theme	genus	142:146	arg1	Rattus					148:153	the genus Rattus	138:153	the genus Rattus	138:153	Because rodents from the genus Rattus are resistant to spontaneous periodontitis, experimental periodontitis must be initiated by mechanical procedures and interventions.					
31575381	11	62	theme	HSC	1495:1497	arg1	diet					1499:1502	HSC diet	1495:1502	HSC diet for 24 wk	1495:1512	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	6	63	theme	age	867:869	arg1	wk					861:862	29 wk	858:862	29 wk of age	858:869	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	5	64	theme	baseline	682:689	arg1	BSL					698:700	BSL	698:700	BSL	698:700	A baseline group (BSL, n = 8) was euthanized at age 6 wk.					
31575381	5	64	theme	baseline	682:689	arg1	group					691:695	A baseline group	680:695	A baseline group (BSL, n = 8)	680:708	A baseline group (BSL, n = 8) was euthanized at age 6 wk.					
31575381	11	65	theme	alveolar	1734:1741	arg1	bone					1743:1746	alveolar bone	1734:1746	alveolar bone	1734:1746	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	7	66	theme	mandibles	955:963	arg1	μCT					935:937	μCT	935:937	μCT	935:937	We evaluated the degree of periodontitis through histology and μCT of maxillae and mandibles.					
31575381	7	66	theme	mandibles	955:963	arg1	histology					921:929	histology	921:929	histology	921:929	We evaluated the degree of periodontitis through histology and μCT of maxillae and mandibles.					
31575381	6	67	theme	age	759:761	arg1	wk					753:754	6 wk	751:754	6 wk of age	751:761	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	3	68	theme	Th1	313:315	arg1	response					317:324	their exacerbated Th1 response	295:324	their exacerbated Th1 response	295:324	Due to their exacerbated Th1 response and imbalanced Th17 regulatory T-cell responses, Lewis rats are highly susceptible to inducible inflammatory and autoimmune diseases.					
31575381	7	69	theme	maxillae	942:949	arg1	μCT					935:937	μCT	935:937	μCT	935:937	We evaluated the degree of periodontitis through histology and μCT of maxillae and mandibles.					
31575381	7	69	theme	maxillae	942:949	arg1	histology					921:929	histology	921:929	histology	921:929	We evaluated the degree of periodontitis through histology and μCT of maxillae and mandibles.					
31575381	9	70	theme	immunity	1169:1176	arg1	cytokines					1178:1186	innate immunity cytokines	1162:1186	innate immunity cytokines	1162:1186	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	13	71	from	presentation	2100:2111	arg1	similar					2089:2095	similar	2089:2095	similar	2089:2095	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	13	71	from	presentation	2100:2111	arg1	approach					2028:2035	a promising approach	2016:2035	a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease	2016:2132	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	13	71	from	presentation	2100:2111	arg1	model					1985:1989	the rat model	1977:1989	the rat model we described here	1977:2007	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	10	72	from	concentrations	1397:1410	arg1	liver					1466:1470	the liver	1462:1470	the liver	1462:1470	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	3	73	theme	Th17	341:344	arg1	responses					364:372	imbalanced Th17 regulatory T-cell responses	330:372	imbalanced Th17 regulatory T-cell responses	330:372	Due to their exacerbated Th1 response and imbalanced Th17 regulatory T-cell responses, Lewis rats are highly susceptible to inducible inflammatory and autoimmune diseases.					
31575381	9	74	theme	Th17	1146:1149	arg1	responses					1151:1159	Th1 and Th17 responses	1138:1159	Th1 and Th17 responses	1138:1159	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	4	75	theme	feeding	481:487	arg1	rats					495:498	feeding Lewis rats a diet high in sucrose and casein (HSC)	481:538	feeding Lewis rats a diet high in sucrose and casein (HSC)	481:538	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	9	76	theme	tissue	1193:1198	arg1	damage					1200:1205	tissue damage	1193:1205	tissue damage in response to bacteria	1193:1229	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	10	77	from	expression	1422:1431	arg1	liver					1466:1470	the liver	1462:1470	the liver	1462:1470	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	13	78	dep	moderate	2058:2065	arg1	to					2055:2056	to	2055:2056	to	2055:2056	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	12	79	theme	mechanical	1858:1867	arg1	intervention					1869:1880	mechanical intervention	1858:1880	mechanical intervention in the oral cavity	1858:1899	HSC-fed Lewis rats developed periodontitis without mechanical intervention in the oral cavity and in the absence of any noteworthy metabolic abnormalities.					
31575381	3	80	theme	T-cell	357:362	arg1	responses					364:372	imbalanced Th17 regulatory T-cell responses	330:372	imbalanced Th17 regulatory T-cell responses	330:372	Due to their exacerbated Th1 response and imbalanced Th17 regulatory T-cell responses, Lewis rats are highly susceptible to inducible inflammatory and autoimmune diseases.					
31575381	10	81	theme	diet	1289:1292	arg1	effects					1274:1280	The potential systemic effects	1251:1280	The potential systemic effects of HSC diet	1251:1292	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	1	82	theme	important	74:82	arg1	worldwide					106:114	an important public health concern worldwide	71:114	an important public health concern worldwide	71:114	Periodontitis is an important public health concern worldwide.					
31575381	1	82	theme	important	74:82	arg1	Periodontitis					54:66	Periodontitis	54:66	Periodontitis	54:66	Periodontitis is an important public health concern worldwide.					
31575381	10	83	theme	insulin	1389:1395	arg1	concentrations					1397:1410	serum leptin and insulin concentrations	1372:1410	concentrations	1397:1410	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	0	84	theme	Generalized	13:23	arg1	Periodontitis					25:37	Diet-induced Generalized Periodontitis	0:37	Diet-induced Generalized Periodontitis in Lewis Rats	0:51	Diet-induced Generalized Periodontitis in Lewis Rats.					
31575381	1	85	theme	health	91:96	arg1	worldwide					106:114	an important public health concern worldwide	71:114	an important public health concern worldwide	71:114	Periodontitis is an important public health concern worldwide.					
31575381	1	85	theme	health	91:96	arg1	Periodontitis					54:66	Periodontitis	54:66	Periodontitis	54:66	Periodontitis is an important public health concern worldwide.					
31575381	11	86	theme	inflammatory	1637:1648	arg1	infiltration					1655:1666	inflammatory cell infiltration	1637:1666	inflammatory cell infiltration (predominantly T cells and macrophages)	1637:1706	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	8	87	theme	HSC-induced	970:980	arg1	response					995:1002	The HSC-induced inflammatory response	966:1002	The HSC-induced inflammatory response of periodontal tissues	966:1025	The HSC-induced inflammatory response of periodontal tissues was assessed by using immunohistochemistry.					
31575381	10	88	theme	HSC	1285:1287	arg1	diet					1289:1292	HSC diet	1285:1292	HSC diet	1285:1292	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	6	89	dep	STD	806:808	arg1	n					811:811	n = 12	811:816	n = 12	811:816	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	10	90	theme	gene	1417:1420	arg1	expression					1422:1431	gene expression	1417:1431	gene expression of inflammatory cytokines in the liver	1417:1470	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	12	91	theme	HSC-fed	1807:1813	arg1	rats					1821:1824	HSC-fed Lewis rats	1807:1824	HSC-fed Lewis rats	1807:1824	HSC-fed Lewis rats developed periodontitis without mechanical intervention in the oral cavity and in the absence of any noteworthy metabolic abnormalities.					
31575381	6	92	theme	=	813:813	arg1	n					811:811	n = 12	811:816	n = 12	811:816	Beginning at 6 wk of age, 2 groups of Lewis rats were fed standard (STD, n = 12) or HSC (n = 20) chow and euthanized at 29 wk of age.					
31575381	2	93	theme	spontaneous	172:182	arg1	periodontitis					184:196	spontaneous periodontitis	172:196	spontaneous periodontitis	172:196	Because rodents from the genus Rattus are resistant to spontaneous periodontitis, experimental periodontitis must be initiated by mechanical procedures and interventions.					
31575381	10	94	theme	systemic	1265:1272	arg1	effects					1274:1280	The potential systemic effects	1251:1280	The potential systemic effects of HSC diet	1251:1292	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	8	95	theme	periodontal	1007:1017	arg1	tissues					1019:1025	periodontal tissues	1007:1025	periodontal tissues	1007:1025	The HSC-induced inflammatory response of periodontal tissues was assessed by using immunohistochemistry.					
31575381	3	96	theme	autoimmune	439:448	arg1	diseases					450:457	autoimmune diseases	439:457	autoimmune diseases	439:457	Due to their exacerbated Th1 response and imbalanced Th17 regulatory T-cell responses, Lewis rats are highly susceptible to inducible inflammatory and autoimmune diseases.					
31575381	9	97	dep	bacteria	1222:1229	arg1	response					1210:1217	response	1210:1217	response	1210:1217	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	10	98	theme	cytokines	1449:1457	arg1	composition					1327:1337	body composition	1322:1337	body composition	1322:1337	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	10	98	theme	cytokines	1449:1457	arg1	densitometry					1348:1359	bone densitometry	1343:1359	bone densitometry	1343:1359	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	10	98	theme	cytokines	1449:1457	arg1	expression					1422:1431	gene expression	1417:1431	gene expression of inflammatory cytokines in the liver	1417:1470	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	10	98	theme	cytokines	1449:1457	arg1	leptin					1378:1383	serum leptin and insulin concentrations	1372:1410	leptin	1378:1383	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	10	98	theme	cytokines	1449:1457	arg1	concentrations					1397:1410	serum leptin and insulin concentrations	1372:1410	concentrations	1397:1410	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	11	99	theme	cell	1650:1653	arg1	infiltration					1655:1666	inflammatory cell infiltration	1637:1666	inflammatory cell infiltration (predominantly T cells and macrophages)	1637:1706	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	13	100	theme	moderate	2058:2065	arg1	periodontitis					2067:2079	modeling mild to moderate periodontitis	2041:2079	modeling mild to moderate periodontitis	2041:2079	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
31575381	10	101	dep	composition	1327:1337	arg1	endpoints					1361:1369	endpoints	1361:1369	endpoints	1361:1369	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	11	102	dep	moderate	1584:1591	arg1	to					1581:1582	to	1581:1582	to	1581:1582	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	4	103	theme	lesions	639:645	arg1	development					610:620	the development	606:620	the development of periodontitis lesions without mechanical intervention	606:677	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	4	103	theme	lesions	639:645	arg1	inflammation					589:600	inflammation	589:600	inflammation	589:600	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	11	104	theme	host	1524:1527	arg1	response					1540:1547	a host Th1-immune response	1522:1547	a host Th1-immune response	1522:1547	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	10	105	from	composition	1327:1337	arg1	liver					1466:1470	the liver	1462:1470	the liver	1462:1470	The potential systemic effects of HSC diet were evaluated by assessing body composition and bone densitometry endpoints; serum leptin and insulin concentrations; and gene expression of inflammatory cytokines in the liver.					
31575381	4	106	theme	mechanical	655:664	arg1	intervention					666:677	mechanical intervention	655:677	mechanical intervention	655:677	We hypothesized that feeding Lewis rats a diet high in sucrose and casein (HSC) would alter the oral microenvironment and induce inflammation and the development of periodontitis lesions without mechanical intervention.					
31575381	11	107	theme	periodontal	1552:1562	arg1	periodontitis					1606:1618	generalized periodontitis	1594:1618	generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium	1594:1804	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	11	107	theme	periodontal	1552:1562	arg1	tissues					1564:1570	periodontal tissues	1552:1570	periodontal tissues	1552:1570	Placing Lewis rats on HSC diet for 24 wk induced a host Th1-immune response in periodontal tissues and mild to moderate, generalized periodontitis characterized by inflammatory cell infiltration (predominantly T cells and macrophages), osteoclast resorption of alveolar bone, and hyperplasia and migration of the gingival epithelium.					
31575381	9	108	theme	expression	1076:1085	arg1	analysis					1087:1094	Gene expression analysis	1071:1094	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria	1071:1229	Gene expression analysis of inflammatory cytokines associated with Th1 and Th17 responses, innate immunity cytokines, and tissue damage in response to bacteria were assessed also.					
31575381	13	109	theme	human	2120:2124	arg1	disease					2126:2132	the human disease	2116:2132	the human disease	2116:2132	Consequently, the rat model we described here may be a promising approach for modeling mild to moderate periodontitis that is similar in presentation to the human disease.					
29943280	7	0	theme	different	1210:1218	arg1	composition					1220:1230	different composition	1210:1230	different composition	1210:1230	Organoleptic differences were ascribed to different composition and physical properties of excipients, resulting in dissimilar taste and mouth-feel.					
29943280	3	1	theme	suitable	359:366	arg1	excipient					381:389	the most suitable co-processed excipient	350:389	the most suitable co-processed excipient to use within directly compressible dispersible tablet formulations	350:457	The aim of this study was to identify the most suitable co-processed excipient to use within directly compressible dispersible tablet formulations.					
29943280	4	2	theme	successful	495:504	arg1	manufacturability					506:522	successful manufacturability	495:522	successful manufacturability	495:522	Nine excipients, selected based on successful manufacturability, were investigated in a randomised, preference and acceptability testing in 24 healthy adult volunteers.					
29943280	5	3	theme	preference	668:677	arg1	order					659:663	order	659:663	order of preference	659:677	Excipients were classified in order of preference as follows (from most preferred): SmartEx QD100 > F-Melt Type C > F-Melt Type M > MicroceLac > Ludiflash > CombiLac > Pharmaburst 500 > Avicel HFE-102 > Avicel PH-102.					
29943280	8	4	theme	maximum	1470:1476	arg1	size					1487:1490	maximum particle size	1470:1490	maximum particle size of dispersible formulation	1470:1517	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	6	5	theme	Avicel	1076:1081	arg1	products					1083:1090	Avicel products	1076:1090	Avicel products	1076:1090	Broad differences were identified in terms of acceptability, with SmartEx QD100 being 'very acceptable', F-Melt Type C, F-Melt Type M and MicroceLac being 'acceptable', Ludiflash, CombiLac and Pharmaburst 500 being 'neutral' and Avicel products being 'very unacceptable' based on ratings using five-point hedonic scales.					
29943280	7	6	theme	excipients	1259:1268	arg1	properties					1245:1254	physical properties	1236:1254	physical properties	1236:1254	Organoleptic differences were ascribed to different composition and physical properties of excipients, resulting in dissimilar taste and mouth-feel.					
29943280	7	6	theme	excipients	1259:1268	arg1	composition					1220:1230	different composition	1210:1230	different composition	1210:1230	Organoleptic differences were ascribed to different composition and physical properties of excipients, resulting in dissimilar taste and mouth-feel.					
29943280	8	7	theme	value	1445:1449	arg1	use					1433:1435	the use	1429:1435	the use of this value as a threshold for maximum particle size of dispersible formulation	1429:1517	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	1	8	theme	critical	122:129	arg1	Palatability					79:90	Palatability	79:90	Palatability	79:90	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	1	8	theme	critical	122:129	arg1	acceptability					104:116	patient acceptability	96:116	patient acceptability	96:116	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	1	8	theme	critical	122:129	arg1	attributes					131:140	critical attributes	122:140	critical attributes of dispersible tablet formulation	122:174	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	9	9	theme	most	1524:1527	arg1	excipients					1552:1561	The most promising co-processed excipients	1520:1561	The most promising co-processed excipients for directly compressible dispersible tablets	1520:1607	The most promising co-processed excipients for directly compressible dispersible tablets were successfully identified.					
29943280	6	10	theme	Broad	847:851	arg1	differences					853:863	Broad differences	847:863	Broad differences	847:863	Broad differences were identified in terms of acceptability, with SmartEx QD100 being 'very acceptable', F-Melt Type C, F-Melt Type M and MicroceLac being 'acceptable', Ludiflash, CombiLac and Pharmaburst 500 being 'neutral' and Avicel products being 'very unacceptable' based on ratings using five-point hedonic scales.					
29943280	9	11	theme	promising	1529:1537	arg1	excipients					1552:1561	The most promising co-processed excipients	1520:1561	The most promising co-processed excipients for directly compressible dispersible tablets	1520:1607	The most promising co-processed excipients for directly compressible dispersible tablets were successfully identified.					
29943280	7	12	theme	dissimilar	1284:1293	arg1	taste					1295:1299	dissimilar taste	1284:1299	dissimilar taste	1284:1299	Organoleptic differences were ascribed to different composition and physical properties of excipients, resulting in dissimilar taste and mouth-feel.					
29943280	2	13	theme	Co-processed	177:188	arg1	excipients					190:199	Co-processed excipients	177:199	Co-processed excipients	177:199	Co-processed excipients could provide improved organoleptic profile due to rational choice of excipients and manufacturing techniques.					
29943280	8	14	dep	200-250 μm	1368:1377	arg1	larger					1356:1361	larger	1356:1361	larger	1356:1361	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	8	14	dep	200-250 μm	1368:1377	arg1	than					1363:1366	than	1363:1366	than	1363:1366	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	9	15	theme	co-processed	1539:1550	arg1	excipients					1552:1561	The most promising co-processed excipients	1520:1561	The most promising co-processed excipients for directly compressible dispersible tablets	1520:1607	The most promising co-processed excipients for directly compressible dispersible tablets were successfully identified.					
29943280	9	16	theme	compressible	1576:1587	arg1	tablets					1601:1607	directly compressible dispersible tablets	1567:1607	directly compressible dispersible tablets	1567:1607	The most promising co-processed excipients for directly compressible dispersible tablets were successfully identified.					
29943280	8	17	from	Excipients	1317:1326	arg1	water					1350:1354	water	1350:1354	water larger than 200-250 μm	1350:1377	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	6	18	theme	hedonic	1152:1158	arg1	scales					1160:1165	five-point hedonic scales	1141:1165	five-point hedonic scales	1141:1165	Broad differences were identified in terms of acceptability, with SmartEx QD100 being 'very acceptable', F-Melt Type C, F-Melt Type M and MicroceLac being 'acceptable', Ludiflash, CombiLac and Pharmaburst 500 being 'neutral' and Avicel products being 'very unacceptable' based on ratings using five-point hedonic scales.					
29943280	3	19	theme	tablet	439:444	arg1	formulations					446:457	directly compressible dispersible tablet formulations	405:457	directly compressible dispersible tablet formulations	405:457	The aim of this study was to identify the most suitable co-processed excipient to use within directly compressible dispersible tablet formulations.					
29943280	4	20	theme	randomised	548:557	arg1	preference					560:569	preference	560:569	preference	560:569	Nine excipients, selected based on successful manufacturability, were investigated in a randomised, preference and acceptability testing in 24 healthy adult volunteers.					
29943280	1	21	theme	patient	96:102	arg1	Palatability					79:90	Palatability	79:90	Palatability	79:90	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	1	21	theme	patient	96:102	arg1	acceptability					104:116	patient acceptability	96:116	patient acceptability	96:116	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	1	21	theme	patient	96:102	arg1	attributes					131:140	critical attributes	122:140	critical attributes of dispersible tablet formulation	122:174	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	6	22	theme	five-point	1141:1150	arg1	scales					1160:1165	five-point hedonic scales	1141:1165	five-point hedonic scales	1141:1165	Broad differences were identified in terms of acceptability, with SmartEx QD100 being 'very acceptable', F-Melt Type C, F-Melt Type M and MicroceLac being 'acceptable', Ludiflash, CombiLac and Pharmaburst 500 being 'neutral' and Avicel products being 'very unacceptable' based on ratings using five-point hedonic scales.					
29943280	0	23	theme	Co-Processed	0:11	arg1	Excipients					13:22	Co-Processed Excipients	0:22	Co-Processed Excipients for Dispersible Tablets-Part 2	0:53	Co-Processed Excipients for Dispersible Tablets-Part 2: Patient Acceptability.					
29943280	7	24	theme	Organoleptic	1168:1179	arg1	differences					1181:1191	Organoleptic differences	1168:1191	Organoleptic differences	1168:1191	Organoleptic differences were ascribed to different composition and physical properties of excipients, resulting in dissimilar taste and mouth-feel.					
29943280	6	25	theme	Type	974:977	arg1	M					979:979	F-Melt Type M	967:979	F-Melt Type M	967:979	Broad differences were identified in terms of acceptability, with SmartEx QD100 being 'very acceptable', F-Melt Type C, F-Melt Type M and MicroceLac being 'acceptable', Ludiflash, CombiLac and Pharmaburst 500 being 'neutral' and Avicel products being 'very unacceptable' based on ratings using five-point hedonic scales.					
29943280	3	26	theme	compressible	414:425	arg1	formulations					446:457	directly compressible dispersible tablet formulations	405:457	directly compressible dispersible tablet formulations	405:457	The aim of this study was to identify the most suitable co-processed excipient to use within directly compressible dispersible tablet formulations.					
29943280	8	27	theme	particle	1478:1485	arg1	size					1487:1490	maximum particle size	1470:1490	maximum particle size of dispersible formulation	1470:1517	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	8	28	theme	200-250 μm	1368:1377	arg1	water					1350:1354	water	1350:1354	water larger than 200-250 μm	1350:1377	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	3	29	theme	dispersible	427:437	arg1	formulations					446:457	directly compressible dispersible tablet formulations	405:457	directly compressible dispersible tablet formulations	405:457	The aim of this study was to identify the most suitable co-processed excipient to use within directly compressible dispersible tablet formulations.					
29943280	3	30	theme	study	328:332	arg1	aim					316:318	The aim	312:318	The aim of this study	312:332	The aim of this study was to identify the most suitable co-processed excipient to use within directly compressible dispersible tablet formulations.					
29943280	2	31	theme	organoleptic	224:235	arg1	profile					237:243	improved organoleptic profile	215:243	improved organoleptic profile due to rational choice of excipients and manufacturing techniques	215:309	Co-processed excipients could provide improved organoleptic profile due to rational choice of excipients and manufacturing techniques.					
29943280	0	32	dep	Acceptability	64:76	arg1	Excipients					13:22	Co-Processed Excipients	0:22	Co-Processed Excipients for Dispersible Tablets-Part 2	0:53	Co-Processed Excipients for Dispersible Tablets-Part 2: Patient Acceptability.					
29943280	9	33	theme	dispersible	1589:1599	arg1	tablets					1601:1607	directly compressible dispersible tablets	1567:1607	directly compressible dispersible tablets	1567:1607	The most promising co-processed excipients for directly compressible dispersible tablets were successfully identified.					
29943280	2	34	theme	improved	215:222	arg1	profile					237:243	improved organoleptic profile	215:243	improved organoleptic profile due to rational choice of excipients and manufacturing techniques	215:309	Co-processed excipients could provide improved organoleptic profile due to rational choice of excipients and manufacturing techniques.					
29943280	6	35	theme	F-Melt	967:972	arg1	M					979:979	F-Melt Type M	967:979	F-Melt Type M	967:979	Broad differences were identified in terms of acceptability, with SmartEx QD100 being 'very acceptable', F-Melt Type C, F-Melt Type M and MicroceLac being 'acceptable', Ludiflash, CombiLac and Pharmaburst 500 being 'neutral' and Avicel products being 'very unacceptable' based on ratings using five-point hedonic scales.					
29943280	2	36	theme	excipients	271:280	arg1	choice					261:266	rational choice	252:266	rational choice of excipients and manufacturing techniques	252:309	Co-processed excipients could provide improved organoleptic profile due to rational choice of excipients and manufacturing techniques.					
29943280	0	37	theme	Patient	56:62	arg1	Acceptability					64:76	Patient Acceptability	56:76	Patient Acceptability	56:76	Co-Processed Excipients for Dispersible Tablets-Part 2: Patient Acceptability.					
29943280	8	38	theme	formulation	1507:1517	arg1	size					1487:1490	maximum particle size	1470:1490	maximum particle size of dispersible formulation	1470:1517	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	6	39	theme	acceptability	893:905	arg1	terms					884:888	terms	884:888	terms of acceptability	884:905	Broad differences were identified in terms of acceptability, with SmartEx QD100 being 'very acceptable', F-Melt Type C, F-Melt Type M and MicroceLac being 'acceptable', Ludiflash, CombiLac and Pharmaburst 500 being 'neutral' and Avicel products being 'very unacceptable' based on ratings using five-point hedonic scales.					
29943280	7	40	theme	physical	1236:1243	arg1	properties					1245:1254	physical properties	1236:1254	physical properties	1236:1254	Organoleptic differences were ascribed to different composition and physical properties of excipients, resulting in dissimilar taste and mouth-feel.					
29943280	4	41	theme	adult	611:615	arg1	volunteers					617:626	24 healthy adult volunteers	600:626	24 healthy adult volunteers	600:626	Nine excipients, selected based on successful manufacturability, were investigated in a randomised, preference and acceptability testing in 24 healthy adult volunteers.					
29943280	2	42	theme	rational	252:259	arg1	choice					261:266	rational choice	252:266	rational choice of excipients and manufacturing techniques	252:309	Co-processed excipients could provide improved organoleptic profile due to rational choice of excipients and manufacturing techniques.					
29943280	8	43	theme	dispersible	1495:1505	arg1	formulation					1507:1517	dispersible formulation	1495:1517	dispersible formulation	1495:1517	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	5	44	dep	follows	682:688	arg1	HFE-102 > Avicel					822:837	HFE-102 > Avicel	822:837	HFE-102 > Avicel	822:837	Excipients were classified in order of preference as follows (from most preferred): SmartEx QD100 > F-Melt Type C > F-Melt Type M > MicroceLac > Ludiflash > CombiLac > Pharmaburst 500 > Avicel HFE-102 > Avicel PH-102.					
29943280	4	45	theme	healthy	603:609	arg1	volunteers					617:626	24 healthy adult volunteers	600:626	24 healthy adult volunteers	600:626	Nine excipients, selected based on successful manufacturability, were investigated in a randomised, preference and acceptability testing in 24 healthy adult volunteers.					
29943280	6	46	theme	Type	959:962	arg1	C					964:964	F-Melt Type C	952:964	F-Melt Type C	952:964	Broad differences were identified in terms of acceptability, with SmartEx QD100 being 'very acceptable', F-Melt Type C, F-Melt Type M and MicroceLac being 'acceptable', Ludiflash, CombiLac and Pharmaburst 500 being 'neutral' and Avicel products being 'very unacceptable' based on ratings using five-point hedonic scales.					
29943280	1	47	theme	dispersible	145:155	arg1	formulation					164:174	dispersible tablet formulation	145:174	dispersible tablet formulation	145:174	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	2	48	theme	due	245:247	arg1	profile					237:243	improved organoleptic profile	215:243	improved organoleptic profile due to rational choice of excipients and manufacturing techniques	215:309	Co-processed excipients could provide improved organoleptic profile due to rational choice of excipients and manufacturing techniques.					
29943280	1	49	theme	tablet	157:162	arg1	formulation					164:174	dispersible tablet formulation	145:174	dispersible tablet formulation	145:174	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	3	50	theme	co-processed	368:379	arg1	excipient					381:389	the most suitable co-processed excipient	350:389	the most suitable co-processed excipient to use within directly compressible dispersible tablet formulations	350:457	The aim of this study was to identify the most suitable co-processed excipient to use within directly compressible dispersible tablet formulations.					
29943280	2	51	theme	techniques	300:309	arg1	choice					261:266	rational choice	252:266	rational choice of excipients and manufacturing techniques	252:309	Co-processed excipients could provide improved organoleptic profile due to rational choice of excipients and manufacturing techniques.					
29943280	6	52	theme	F-Melt	952:957	arg1	C					964:964	F-Melt Type C	952:964	F-Melt Type C	952:964	Broad differences were identified in terms of acceptability, with SmartEx QD100 being 'very acceptable', F-Melt Type C, F-Melt Type M and MicroceLac being 'acceptable', Ludiflash, CombiLac and Pharmaburst 500 being 'neutral' and Avicel products being 'very unacceptable' based on ratings using five-point hedonic scales.					
29943280	1	53	theme	formulation	164:174	arg1	Palatability					79:90	Palatability	79:90	Palatability	79:90	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	1	53	theme	formulation	164:174	arg1	acceptability					104:116	patient acceptability	96:116	patient acceptability	96:116	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	1	53	theme	formulation	164:174	arg1	attributes					131:140	critical attributes	122:140	critical attributes of dispersible tablet formulation	122:174	Palatability and patient acceptability are critical attributes of dispersible tablet formulation.					
29943280	8	54	theme	particle	1333:1340	arg1	size					1342:1345	particle size	1333:1345	particle size	1333:1345	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
29943280	2	55	theme	manufacturing	286:298	arg1	techniques					300:309	manufacturing techniques	286:309	manufacturing techniques	286:309	Co-processed excipients could provide improved organoleptic profile due to rational choice of excipients and manufacturing techniques.					
29943280	8	56	with	Excipients	1317:1326	arg1	size					1342:1345	particle size	1333:1345	particle size	1333:1345	Excipients with particle size in water larger than 200-250 μm were considered poorly acceptable, which supports the use of this value as a threshold for maximum particle size of dispersible formulation.					
30419267	9	0	theme	Immunohistochemical	1575:1593	arg1	analysis					1595:1602	Immunohistochemical analysis	1575:1602	Immunohistochemical analysis of the retinal tissues of injected eyes	1575:1642	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	7	1	theme	Intravitreal	1166:1177	arg1	injections					1179:1188	Intravitreal injections	1166:1188	Intravitreal injections of the bioengineered thermogel	1166:1219	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	6	2	theme	protein	1116:1122	arg1	bioactivity					1088:1098	bioactivity	1088:1098	bioactivity	1088:1098	The stability and bioactivity of the released protein were similar to those of commercial CNTF.					
30419267	6	2	theme	protein	1116:1122	arg1	stability					1074:1082	stability	1074:1082	stability	1074:1082	The stability and bioactivity of the released protein were similar to those of commercial CNTF.					
30419267	4	3	theme	binding	794:800	arg1	partners					802:809	its peptide binding partners	782:809	its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides	782:955	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	6	4	dep	stability	1074:1082	arg1	The					1070:1072	The	1070:1072	The	1070:1072	The stability and bioactivity of the released protein were similar to those of commercial CNTF.					
30419267	1	5	theme	overdose	224:231	arg1	risks					204:208	the high risks	195:208	the high risks of local drug overdose	195:231	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	1	5	theme	overdose	224:231	arg1	cataracts					237:245	cataracts	237:245	cataracts	237:245	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	0	6	theme	Controlled	0:9	arg1	strategy					19:26	Controlled release strategy	0:26	Controlled release strategy	0:26	Controlled release strategy designed for intravitreal protein delivery to the retina.					
30419267	1	7	theme	retinal	168:174	arg1	degeneration					176:187	retinal degeneration	168:187	retinal degeneration	168:187	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	3	8	theme	model	558:562	arg1	protein					564:570	a model protein	556:570	a model protein for ocular treatment	556:591	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	3	8	theme	model	558:562	arg1	factor					539:544	ciliary neurotrophic factor	518:544	ciliary neurotrophic factor (CNTF)	518:551	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	9	9	theme	eyes	1639:1642	arg1	tissues					1619:1625	the retinal tissues	1607:1625	the retinal tissues of injected eyes	1607:1642	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	7	10	theme	genes	1341:1345	arg1	upregulation					1398:1409	upregulation	1398:1409	upregulation of STAT1 and STAT3 expression	1398:1439	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	10	theme	genes	1341:1345	arg1	downregulation					1305:1318	expected transient downregulation	1286:1318	expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2)	1286:1395	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	10	theme	genes	1341:1345	arg1	upregulation					1446:1457	upregulation	1446:1457	upregulation of STAT3 phosphorylation	1446:1482	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	1	11	theme	high	199:202	arg1	risks					204:208	the high risks	195:208	the high risks of local drug overdose	195:231	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	4	12	theme	fusion	836:841	arg1	protein					843:849	a fusion protein	834:849	a fusion protein with SH3	834:858	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	4	12	theme	fusion	836:841	arg1	CNTF					812:815	CNTF	812:815	CNTF	812:815	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	6	13	theme	commercial	1149:1158	arg1	CNTF					1160:1163	commercial CNTF	1149:1163	commercial CNTF	1149:1163	The stability and bioactivity of the released protein were similar to those of commercial CNTF.					
30419267	8	14	from	hydrogel	1565:1572	arg1	release					1550:1556	intravitreal protein release	1529:1556	intravitreal protein release from a hydrogel	1529:1572	This constitutes the first demonstration of intravitreal protein release from a hydrogel.					
30419267	8	14	from	hydrogel	1565:1572	arg1	demonstration					1512:1524	the first demonstration	1502:1524	the first demonstration of intravitreal protein release from a hydrogel	1502:1572	This constitutes the first demonstration of intravitreal protein release from a hydrogel.					
30419267	9	15	theme	delivery	1682:1689	arg1	vehicle					1691:1697	the delivery vehicle	1678:1697	the delivery vehicle	1678:1697	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	7	16	theme	transient	1295:1303	arg1	downregulation					1305:1318	expected transient downregulation	1286:1318	expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2)	1286:1395	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	2	17	theme	protein	494:500	arg1	delivery					502:509	sustained intravitreal protein delivery	471:509	sustained intravitreal protein delivery	471:509	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	1	18	theme	promising	140:148	arg1	delivery					106:113	Therapeutic protein delivery	86:113	Therapeutic protein delivery directly to the eye	86:133	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	1	18	theme	promising	140:148	arg1	strategy					150:157	a promising strategy	138:157	a promising strategy to treat retinal degeneration	138:187	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	2	19	theme	sustained	471:479	arg1	delivery					502:509	sustained intravitreal protein delivery	471:509	sustained intravitreal protein delivery	471:509	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	1	20	theme	protein	341:347	arg1	strategies					358:367	sustained protein delivery strategies	331:367	sustained protein delivery strategies	331:367	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	7	21	theme	phosphorylation	1468:1482	arg1	upregulation					1398:1409	upregulation	1398:1409	upregulation of STAT1 and STAT3 expression	1398:1439	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	21	theme	phosphorylation	1468:1482	arg1	downregulation					1305:1318	expected transient downregulation	1286:1318	expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2)	1286:1395	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	21	theme	phosphorylation	1468:1482	arg1	upregulation					1446:1457	upregulation	1446:1457	upregulation of STAT3 phosphorylation	1446:1482	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	9	22	theme	intravitreal	1727:1738	arg1	strategies					1757:1766	new intravitreal protein delivery strategies	1723:1766	new intravitreal protein delivery strategies	1723:1766	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	1	23	theme	strategies	358:367	arg1	development					316:326	the development	312:326	the development of sustained protein delivery strategies	312:367	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	9	24	theme	delivery	1748:1755	arg1	strategies					1757:1766	new intravitreal protein delivery strategies	1723:1766	new intravitreal protein delivery strategies	1723:1766	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	1	25	dep	strategy	150:157	arg1	have					279:282	have	279:282	have	279:282	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	4	26	dep	partners	802:809	arg1	expressed					821:829	expressed	821:829	was expressed as a fusion protein with SH3	817:858	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	4	26	dep	partners	802:809	arg1	modified					922:929	modified	922:929	was modified with SH3 binding peptides	918:955	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	1	27	theme	protein	98:104	arg1	strategy					150:157	a promising strategy	138:157	a promising strategy to treat retinal degeneration	138:187	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	1	27	theme	protein	98:104	arg1	delivery					106:113	Therapeutic protein delivery	86:113	Therapeutic protein delivery directly to the eye	86:133	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	7	28	theme	mouse	1267:1271	arg1	retina					1273:1278	the mouse retina	1263:1278	the mouse retina	1263:1278	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	2	29	theme	hydrogel-based	412:425	arg1	systems					435:441	hydrogel-based release systems	412:441	hydrogel-based release systems	412:441	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	2	29	theme	hydrogel-based	412:425	arg1	solution					458:465	a sensible solution	447:465	a sensible solution for sustained intravitreal protein delivery	447:509	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	3	30	theme	intravitreal	624:635	arg1	system					661:666	an intravitreal, affinity-based release system	621:666	system	661:666	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	1	31	theme	limited	284:290	arg1	progress					292:299	limited progress	284:299	limited progress	284:299	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	4	32	theme	SH3	936:938	arg1	peptides					948:955	SH3 binding peptides	936:955	SH3 binding peptides	936:955	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	2	33	theme	intravitreal	481:492	arg1	delivery					502:509	sustained intravitreal protein delivery	471:509	sustained intravitreal protein delivery	471:509	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	0	34	theme	protein	54:60	arg1	delivery					62:69	intravitreal protein delivery	41:69	intravitreal protein delivery to the retina	41:83	Controlled release strategy designed for intravitreal protein delivery to the retina.					
30419267	7	35	theme	STAT3	1424:1428	arg1	upregulation					1398:1409	upregulation	1398:1409	upregulation of STAT1 and STAT3 expression	1398:1439	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	35	theme	STAT3	1424:1428	arg1	downregulation					1305:1318	expected transient downregulation	1286:1318	expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2)	1286:1395	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	35	theme	STAT3	1424:1428	arg1	upregulation					1446:1457	upregulation	1446:1457	upregulation of STAT3 phosphorylation	1446:1482	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	9	36	theme	tissues	1619:1625	arg1	analysis					1595:1602	Immunohistochemical analysis	1575:1602	Immunohistochemical analysis of the retinal tissues of injected eyes	1575:1642	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	7	37	theme	successful	1228:1237	arg1	delivery					1239:1246	successful delivery	1228:1246	successful delivery of CNTF-SH3 to the mouse retina	1228:1278	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	8	38	theme	first	1506:1510	arg1	demonstration					1512:1524	the first demonstration	1502:1524	the first demonstration of intravitreal protein release from a hydrogel	1502:1572	This constitutes the first demonstration of intravitreal protein release from a hydrogel.					
30419267	3	39	theme	system	661:666	arg1	use					614:616	the use	610:616	the use of an intravitreal, affinity-based release system for protein delivery	610:687	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	7	40	theme	STAT1	1414:1418	arg1	upregulation					1398:1409	upregulation	1398:1409	upregulation of STAT1 and STAT3 expression	1398:1439	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	40	theme	STAT1	1414:1418	arg1	downregulation					1305:1318	expected transient downregulation	1286:1318	expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2)	1286:1395	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	40	theme	STAT1	1414:1418	arg1	upregulation					1446:1457	upregulation	1446:1457	upregulation of STAT3 phosphorylation	1446:1482	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	41	theme	thermogel	1211:1219	arg1	injections					1179:1188	Intravitreal injections	1166:1188	Intravitreal injections of the bioengineered thermogel	1166:1219	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	3	42	theme	affinity-based	638:651	arg1	system					661:666	an intravitreal, affinity-based release system	621:666	system	661:666	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	1	43	theme	local	213:217	arg1	overdose					224:231	local drug overdose	213:231	local drug overdose	213:231	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	8	44	theme	protein	1542:1548	arg1	release					1550:1556	intravitreal protein release	1529:1556	intravitreal protein release from a hydrogel	1529:1572	This constitutes the first demonstration of intravitreal protein release from a hydrogel.					
30419267	7	45	dep	STAT1	1414:1418	arg1	expression					1430:1439	expression	1430:1439	expression	1430:1439	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	3	46	theme	protein	672:678	arg1	delivery					680:687	protein delivery	672:687	protein delivery	672:687	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	1	47	contain	have	279:282	arg1	risks					204:208	the high risks	195:208	the high risks of local drug overdose	195:231	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	1	47	contain	have	279:282	arg1	cataracts					237:245	cataracts	237:245	cataracts	237:245	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	1	47	contain	have	279:282	arg2	progress					292:299	limited progress	284:299	limited progress	284:299	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	6	48	theme	released	1107:1114	arg1	protein					1116:1122	the released protein	1103:1122	the released protein	1103:1122	The stability and bioactivity of the released protein were similar to those of commercial CNTF.					
30419267	8	49	from	demonstration	1512:1524	arg1	hydrogel					1565:1572	a hydrogel	1563:1572	a hydrogel	1563:1572	This constitutes the first demonstration of intravitreal protein release from a hydrogel.					
30419267	3	50	theme	neurotrophic	526:537	arg1	protein					564:570	a model protein	556:570	a model protein for ocular treatment	556:591	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	3	50	theme	neurotrophic	526:537	arg1	CNTF					547:550	CNTF	547:550	CNTF	547:550	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	3	50	theme	neurotrophic	526:537	arg1	factor					539:544	ciliary neurotrophic factor	518:544	ciliary neurotrophic factor (CNTF)	518:551	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	4	51	theme	peptide	786:792	arg1	partners					802:809	its peptide binding partners	782:809	its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides	782:955	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	9	52	theme	injected	1630:1637	arg1	eyes					1639:1642	injected eyes	1630:1642	injected eyes	1630:1642	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	2	53	theme	release	427:433	arg1	systems					435:441	hydrogel-based release systems	412:441	hydrogel-based release systems	412:441	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	2	53	theme	release	427:433	arg1	solution					458:465	a sensible solution	447:465	a sensible solution for sustained intravitreal protein delivery	447:509	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	5	54	theme	hydrogel	990:997	arg1	composition					999:1009	the hydrogel composition	986:1009	the hydrogel composition	986:1009	Using a mathematical model, the hydrogel composition was successfully designed to release CNTF-SH3 over 7 days.					
30419267	3	55	theme	ocular	576:581	arg1	treatment					583:591	ocular treatment	576:591	ocular treatment	576:591	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	7	56	theme	phototransduction	1323:1339	arg1	genes					1341:1345	phototransduction genes	1323:1345	phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2)	1323:1395	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	5	57	theme	mathematical	966:977	arg1	model					979:983	a mathematical model	964:983	a mathematical model	964:983	Using a mathematical model, the hydrogel composition was successfully designed to release CNTF-SH3 over 7 days.					
30419267	9	58	theme	vehicle	1691:1697	arg1	biocompatibility					1658:1673	the biocompatibility	1654:1673	the biocompatibility of the delivery vehicle	1654:1697	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	4	59	theme	CNTF	701:704	arg1	release					706:712	CNTF release	701:712	CNTF release	701:712	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	4	60	theme	methylcellulose	895:909	arg1	thermogel					867:875	a thermogel	865:875	a thermogel of hyaluronan and methylcellulose (HAMC)	865:916	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	9	61	theme	retinal	1611:1617	arg1	tissues					1619:1625	the retinal tissues	1607:1625	the retinal tissues of injected eyes	1607:1642	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	7	62	theme	expected	1286:1293	arg1	downregulation					1305:1318	expected transient downregulation	1286:1318	expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2)	1286:1395	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	7	63	dep	rhodopsin	1354:1362	arg1	e.g.					1348:1351	e.g.	1348:1351	e.g.	1348:1351	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	1	64	theme	sustained	331:339	arg1	strategies					358:367	sustained protein delivery strategies	331:367	sustained protein delivery strategies	331:367	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	7	65	theme	STAT3	1462:1466	arg1	phosphorylation					1468:1482	STAT3 phosphorylation	1462:1482	STAT3 phosphorylation	1462:1482	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	9	66	theme	new	1723:1725	arg1	strategies					1757:1766	new intravitreal protein delivery strategies	1723:1766	new intravitreal protein delivery strategies	1723:1766	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	1	67	theme	delivery	349:356	arg1	strategies					358:367	sustained protein delivery strategies	331:367	sustained protein delivery strategies	331:367	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	4	68	theme	hyaluronan	880:889	arg1	thermogel					867:875	a thermogel	865:875	a thermogel of hyaluronan and methylcellulose (HAMC)	865:916	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	4	69	theme	affinity	740:747	arg1	advantage					723:731	advantage	723:731	advantage of the affinity	723:747	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	2	70	theme	vitreous	380:387	arg1	humor					389:393	the vitreous humor	376:393	the vitreous humor itself	376:400	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	2	70	theme	vitreous	380:387	arg1	gel					407:409	a gel	405:409	a gel	405:409	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	0	71	theme	release	11:17	arg1	strategy					19:26	Controlled release strategy	0:26	Controlled release strategy	0:26	Controlled release strategy designed for intravitreal protein delivery to the retina.					
30419267	1	72	theme	Therapeutic	86:96	arg1	strategy					150:157	a promising strategy	138:157	a promising strategy to treat retinal degeneration	138:187	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	1	72	theme	Therapeutic	86:96	arg1	delivery					106:113	Therapeutic protein delivery	86:113	Therapeutic protein delivery directly to the eye	86:133	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	4	73	theme	binding	940:946	arg1	peptides					948:955	SH3 binding peptides	936:955	SH3 binding peptides	936:955	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	1	74	theme	bolus	263:267	arg1	injection					269:277	bolus injection	263:277	bolus injection	263:277	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30419267	4	75	with	protein	843:849	arg1	SH3					856:858	SH3	856:858	SH3	856:858	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	7	76	theme	CNTF-SH3	1251:1258	arg1	delivery					1239:1246	successful delivery	1228:1246	successful delivery of CNTF-SH3 to the mouse retina	1228:1278	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	0	77	theme	intravitreal	41:52	arg1	delivery					62:69	intravitreal protein delivery	41:69	intravitreal protein delivery to the retina	41:83	Controlled release strategy designed for intravitreal protein delivery to the retina.					
30419267	4	78	mod	modified	922:929	arg1	thermogel					867:875	a thermogel	865:875	a thermogel of hyaluronan and methylcellulose (HAMC)	865:916	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	4	78	mod	modified	922:929	arg3	peptides					948:955	SH3 binding peptides	936:955	SH3 binding peptides	936:955	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	2	79	theme	sensible	449:456	arg1	systems					435:441	hydrogel-based release systems	412:441	hydrogel-based release systems	412:441	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	2	79	theme	sensible	449:456	arg1	solution					458:465	a sensible solution	447:465	a sensible solution for sustained intravitreal protein delivery	447:509	Since the vitreous humor itself is a gel, hydrogel-based release systems are a sensible solution for sustained intravitreal protein delivery.					
30419267	9	80	theme	protein	1740:1746	arg1	strategies					1757:1766	new intravitreal protein delivery strategies	1723:1766	new intravitreal protein delivery strategies	1723:1766	Immunohistochemical analysis of the retinal tissues of injected eyes confirmed the biocompatibility of the delivery vehicle, paving the way towards new intravitreal protein delivery strategies.					
30419267	3	81	theme	release	653:659	arg1	system					661:666	an intravitreal, affinity-based release system	621:666	system	661:666	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	7	82	theme	bioengineered	1197:1209	arg1	thermogel					1211:1219	the bioengineered thermogel	1193:1219	the bioengineered thermogel	1193:1219	Intravitreal injections of the bioengineered thermogel showed successful delivery of CNTF-SH3 to the mouse retina, with expected transient downregulation of phototransduction genes (e.g., rhodopsin, S-opsin, M-opsin, Gnat 1 and 2), upregulation of STAT1 and STAT3 expression, and upregulation of STAT3 phosphorylation.					
30419267	8	83	theme	intravitreal	1529:1540	arg1	release					1550:1556	intravitreal protein release	1529:1556	intravitreal protein release from a hydrogel	1529:1572	This constitutes the first demonstration of intravitreal protein release from a hydrogel.					
30419267	4	84	theme	Src	757:759	arg1	homology					761:768	Src homology 3	757:770	Src homology 3 (SH3)	757:776	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	4	84	theme	Src	757:759	arg1	SH3					773:775	SH3	773:775	SH3	773:775	To sustain CNTF release, we took advantage of the affinity between Src homology 3 (SH3) and its peptide binding partners: CNTF was expressed as a fusion protein with SH3, and a thermogel of hyaluronan and methylcellulose (HAMC) was modified with SH3 binding peptides.					
30419267	8	85	theme	release	1550:1556	arg1	demonstration					1512:1524	the first demonstration	1502:1524	the first demonstration of intravitreal protein release from a hydrogel	1502:1572	This constitutes the first demonstration of intravitreal protein release from a hydrogel.					
30419267	3	86	theme	ciliary	518:524	arg1	protein					564:570	a model protein	556:570	a model protein for ocular treatment	556:591	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	3	86	theme	ciliary	518:524	arg1	CNTF					547:550	CNTF	547:550	CNTF	547:550	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	3	86	theme	ciliary	518:524	arg1	factor					539:544	ciliary neurotrophic factor	518:544	ciliary neurotrophic factor (CNTF)	518:551	Using ciliary neurotrophic factor (CNTF) as a model protein for ocular treatment, we investigated the use of an intravitreal, affinity-based release system for protein delivery.					
30419267	1	87	theme	drug	219:222	arg1	overdose					224:231	local drug overdose	213:231	local drug overdose	213:231	Therapeutic protein delivery directly to the eye is a promising strategy to treat retinal degeneration; yet, the high risks of local drug overdose and cataracts associated with bolus injection have limited progress, requiring the development of sustained protein delivery strategies.					
30347275	4	0	theme	chewing	727:733	arg1	gums					735:738	chewing gums	727:738	chewing gums	727:738	CUR MCGs were prepared by the conventional fusion method for making chewing gums.					
30347275	9	1	theme	CUR/SBE-β-CD	1416:1427	arg1	complex					1439:1445	CUR/SBE-β-CD inclusion complex	1416:1445	CUR/SBE-β-CD inclusion complex	1416:1445	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	9	2	theme	complex	1439:1445	arg1	complex					1439:1445	CUR/SBE-β-CD inclusion complex	1416:1445	CUR/SBE-β-CD inclusion complex	1416:1445	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	9	2	theme	complex	1439:1445	arg1	%					1397:1397	50%	1395:1397	50% of either CUR or CUR/SBE-β-CD inclusion complex	1395:1445	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	9	2	theme	complex	1439:1445	arg1	CUR					1409:1411	CUR	1409:1411	CUR	1409:1411	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	3	3	theme	X1	470:471	arg1	polyisobutene					474:486	(X1) polyisobutene	469:486	(X1) polyisobutene	469:486	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	3	theme	X1	470:471	arg1	acetate					504:510	(X2) polyvinyl acetate	489:510	(X2) polyvinyl acetate	489:510	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	3	theme	X1	470:471	arg1	X3					514:515	X3	514:515	X3	514:515	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	4	theme	MCGs	589:592	arg1	properties					571:580	the mechanical properties	556:580	the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design	556:656	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	5	theme	X2	490:491	arg1	polyisobutene					474:486	(X1) polyisobutene	469:486	(X1) polyisobutene	469:486	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	5	theme	X2	490:491	arg1	acetate					504:510	(X2) polyvinyl acetate	489:510	(X2) polyvinyl acetate	489:510	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	1	6	theme	cancer	248:253	arg1	patients					255:262	head and neck cancer patients	234:262	head and neck cancer patients	234:262	Curcumin (CUR) chewing gums have potential therapeutic benefits to head and neck cancer patients.					
30347275	4	7	theme	fusion	702:707	arg1	method					709:714	the conventional fusion method	685:714	the conventional fusion method for making chewing gums	685:738	CUR MCGs were prepared by the conventional fusion method for making chewing gums.					
30347275	7	8	theme	CUR	1210:1212	arg1	levels					1214:1219	PIB and CUR levels	1202:1219	PIB and CUR levels	1202:1219	Elasticity and stiffness of the gums were found to be readily impacted by PIB and CUR levels.					
30347275	1	9	theme	Curcumin	167:174	arg1	gums					190:193	Curcumin (CUR) chewing gums	167:193	Curcumin (CUR) chewing gums	167:193	Curcumin (CUR) chewing gums have potential therapeutic benefits to head and neck cancer patients.					
30347275	7	10	theme	PIB	1202:1204	arg1	levels					1214:1219	PIB and CUR levels	1202:1219	PIB and CUR levels	1202:1219	Elasticity and stiffness of the gums were found to be readily impacted by PIB and CUR levels.					
30347275	2	11	theme	work	287:290	arg1	objective					269:277	The objective	265:277	The objective of this work	265:290	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	9	12	theme	drug	1471:1474	arg1	release					1476:1482	drug release	1471:1482	drug release	1471:1482	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	9	13	theme	optimized	1365:1373	arg1	MCG					1375:1377	The optimized MCG	1361:1377	The optimized MCG	1361:1377	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	3	14	dep	design	651:656	arg1	five-factor					610:620	a 25-run, five-factor, two-level D-Optimal mixture design	600:656	five-factor	610:620	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	10	15	theme	substituting	1549:1560	arg1	CUR					1562:1564	substituting CUR	1549:1564	substituting CUR with the inclusion complex	1549:1591	Although no differences in mechanical properties were observed, substituting CUR with the inclusion complex was found to significantly enhance drug release.					
30347275	1	16	theme	chewing	182:188	arg1	gums					190:193	Curcumin (CUR) chewing gums	167:193	Curcumin (CUR) chewing gums	167:193	Curcumin (CUR) chewing gums have potential therapeutic benefits to head and neck cancer patients.					
30347275	3	17	theme	D-Optimal	633:641	arg1	design					651:656	a 25-run, five-factor, two-level D-Optimal mixture design	600:656	design	651:656	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	0	18	theme	B	137:137	arg1	elastomer					142:150	oppanol® B 12 elastomer	128:150	oppanol® B 12 elastomer	128:150	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	0	18	theme	B	137:137	arg1	gum-base					157:164	a gum-base	155:164	a gum-base	155:164	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	3	19	theme	wood	518:521	arg1	ingredients					456:466	five gum ingredients	447:466	five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR	447:551	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	19	theme	wood	518:521	arg1	rosin					523:527	(X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin	469:527	(X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin	469:527	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	6	20	theme	Observed	1011:1018	arg1	responses					1020:1028	Observed responses	1011:1028	Observed responses	1011:1028	Observed responses were used to generate polynomial models correlating the independent with the dependent variables.					
30347275	6	21	theme	dependent	1107:1115	arg1	variables					1117:1125	the dependent variables	1103:1125	the dependent variables	1103:1125	Observed responses were used to generate polynomial models correlating the independent with the dependent variables.					
30347275	10	22	theme	inclusion	1575:1583	arg1	complex					1585:1591	the inclusion complex	1571:1591	the inclusion complex	1571:1591	Although no differences in mechanical properties were observed, substituting CUR with the inclusion complex was found to significantly enhance drug release.					
30347275	5	23	theme	two-bites	770:778	arg1	texture					780:786	a two-bites texture	768:786	a two-bites texture	768:786	They were characterized by a two-bites texture and uniaxial tension tests to generate force-displacement curves from which the cohesiveness (Y1), springiness (Y2), chewiness (Y3), compressibility (Y4), resistance to extension (Y5), and extensibility (Y6) were measured.					
30347275	7	24	theme	gums	1160:1163	arg1	Elasticity					1128:1137	Elasticity	1128:1137	Elasticity	1128:1137	Elasticity and stiffness of the gums were found to be readily impacted by PIB and CUR levels.					
30347275	7	24	theme	gums	1160:1163	arg1	stiffness					1143:1151	stiffness	1143:1151	stiffness	1143:1151	Elasticity and stiffness of the gums were found to be readily impacted by PIB and CUR levels.					
30347275	3	25	theme	X4	531:532	arg1	ingredients					456:466	five gum ingredients	447:466	five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR	447:551	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	25	theme	X4	531:532	arg1	wax					535:537	(X4) wax	530:537	(X4) wax	530:537	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	11	26	dep	%	1798:1798	arg1	50					1796:1797	50	1796:1797	50	1796:1797	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	3	27	theme	ingredients	456:466	arg1	effect					437:442	the effect	433:442	the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design	433:656	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	11	28	dep	loading	1805:1811	arg1	%					1798:1798	%	1798:1798	%	1798:1798	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	11	29	theme	chewing	1772:1778	arg1	gums					1780:1783	chewing gums	1772:1783	chewing gums with up to 50% drug loading	1772:1811	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	5	30	theme	force-displacement	827:844	arg1	curves					846:851	force-displacement curves	827:851	force-displacement curves from which the cohesiveness (Y1), springiness (Y2), chewiness (Y3), compressibility (Y4), resistance to extension (Y5), and extensibility (Y6) were measured	827:1008	They were characterized by a two-bites texture and uniaxial tension tests to generate force-displacement curves from which the cohesiveness (Y1), springiness (Y2), chewiness (Y3), compressibility (Y4), resistance to extension (Y5), and extensibility (Y6) were measured.					
30347275	8	31	theme	mechanical	1300:1309	arg1	properties					1311:1320	comparable mechanical properties	1289:1320	comparable mechanical properties	1289:1320	Fitted models were then used to predict a gum composition that has comparable mechanical properties to commercially procured chewing gums.					
30347275	11	32	theme	ingredient	1688:1697	arg1	impact					1669:1674	the impact	1665:1674	the impact of each gum ingredient on the quality of the MCGs	1665:1724	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	0	33	theme	chewing	109:115	arg1	gums					117:120	curcumin chewing gums	100:120	curcumin chewing gums using oppanol® B 12 elastomer as a gum-base	100:164	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	3	34	dep	ingredients	456:466	arg1	ingredients					456:466	five gum ingredients	447:466	five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR	447:551	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	34	dep	ingredients	456:466	arg1	wax					535:537	(X4) wax	530:537	(X4) wax	530:537	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	34	dep	ingredients	456:466	arg1	rosin					523:527	(X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin	469:527	(X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin	469:527	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	34	dep	ingredients	456:466	arg1	CUR					549:551	(X5) CUR	544:551	(X5) CUR	544:551	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	2	35	theme	high	342:345	arg1	loading					351:357	high CUR loading	342:357	high CUR loading	342:357	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	0	36	theme	D-optimal	0:8	arg1	design					18:23	D-optimal mixture design	0:23	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.	0:165	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	5	37	theme	tension	801:807	arg1	tests					809:813	uniaxial tension tests	792:813	uniaxial tension tests	792:813	They were characterized by a two-bites texture and uniaxial tension tests to generate force-displacement curves from which the cohesiveness (Y1), springiness (Y2), chewiness (Y3), compressibility (Y4), resistance to extension (Y5), and extensibility (Y6) were measured.					
30347275	8	38	theme	procured	1338:1345	arg1	gums					1355:1358	commercially procured chewing gums	1325:1358	commercially procured chewing gums	1325:1358	Fitted models were then used to predict a gum composition that has comparable mechanical properties to commercially procured chewing gums.					
30347275	2	39	theme	desirable	363:371	arg1	properties					385:394	desirable mastication properties	363:394	desirable mastication properties	363:394	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	0	40	theme	Formulation	26:36	arg1	development					38:48	Formulation development	26:48	Formulation development	26:48	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	3	41	theme	gum	452:454	arg1	ingredients					456:466	five gum ingredients	447:466	five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR	447:551	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	41	theme	gum	452:454	arg1	wax					535:537	(X4) wax	530:537	(X4) wax	530:537	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	41	theme	gum	452:454	arg1	rosin					523:527	(X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin	469:527	(X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin	469:527	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	41	theme	gum	452:454	arg1	CUR					549:551	(X5) CUR	544:551	(X5) CUR	544:551	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	42	from	effect	437:442	arg1	properties					571:580	the mechanical properties	556:580	the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design	556:656	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	1	43	theme	potential	200:208	arg1	benefits					222:229	potential therapeutic benefits	200:229	potential therapeutic benefits	200:229	Curcumin (CUR) chewing gums have potential therapeutic benefits to head and neck cancer patients.					
30347275	0	44	dep	design	18:23	arg1	development					38:48	Formulation development	26:48	Formulation development	26:48	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	0	44	dep	design	18:23	arg1	characterization					62:77	mechanical characterization	51:77	mechanical characterization	51:77	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	0	44	dep	design	18:23	arg1	optimization					84:95	optimization	84:95	optimization	84:95	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	3	45	theme	X5	545:546	arg1	ingredients					456:466	five gum ingredients	447:466	five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR	447:551	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	45	theme	X5	545:546	arg1	CUR					549:551	(X5) CUR	544:551	(X5) CUR	544:551	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	8	46	theme	Fitted	1222:1227	arg1	models					1229:1234	Fitted models	1222:1234	Fitted models	1222:1234	Fitted models were then used to predict a gum composition that has comparable mechanical properties to commercially procured chewing gums.					
30347275	10	47	theme	mechanical	1512:1521	arg1	properties					1523:1532	mechanical properties	1512:1532	mechanical properties	1512:1532	Although no differences in mechanical properties were observed, substituting CUR with the inclusion complex was found to significantly enhance drug release.					
30347275	1	48	theme	head	234:237	arg1	patients					255:262	head and neck cancer patients	234:262	head and neck cancer patients	234:262	Curcumin (CUR) chewing gums have potential therapeutic benefits to head and neck cancer patients.					
30347275	9	49	theme	inclusion	1429:1437	arg1	complex					1439:1445	CUR/SBE-β-CD inclusion complex	1416:1445	CUR/SBE-β-CD inclusion complex	1416:1445	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	1	50	theme	neck	243:246	arg1	patients					255:262	head and neck cancer patients	234:262	head and neck cancer patients	234:262	Curcumin (CUR) chewing gums have potential therapeutic benefits to head and neck cancer patients.					
30347275	2	51	theme	chewing	317:323	arg1	MCGs					331:334	MCGs	331:334	MCGs	331:334	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	2	51	theme	chewing	317:323	arg1	gums					325:328	medicated chewing gums	307:328	medicated chewing gums (MCGs) with high CUR loading	307:357	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	6	52	theme	polynomial	1052:1061	arg1	models					1063:1068	polynomial models	1052:1068	polynomial models correlating the independent with the dependent variables	1052:1125	Observed responses were used to generate polynomial models correlating the independent with the dependent variables.					
30347275	3	53	theme	polyisobutene	474:486	arg1	ingredients					456:466	five gum ingredients	447:466	five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR	447:551	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	53	theme	polyisobutene	474:486	arg1	rosin					523:527	(X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin	469:527	(X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin	469:527	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	2	54	with	properties	385:394	arg1	loading					351:357	high CUR loading	342:357	high CUR loading	342:357	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	4	55	theme	conventional	689:700	arg1	method					709:714	the conventional fusion method	685:714	the conventional fusion method for making chewing gums	685:738	CUR MCGs were prepared by the conventional fusion method for making chewing gums.					
30347275	10	56	from	differences	1497:1507	arg1	properties					1523:1532	mechanical properties	1512:1532	mechanical properties	1512:1532	Although no differences in mechanical properties were observed, substituting CUR with the inclusion complex was found to significantly enhance drug release.					
30347275	11	57	theme	drug	1800:1803	arg1	loading					1805:1811	up to 50% drug loading	1790:1811	up to 50% drug loading	1790:1811	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	8	58	contain	has	1285:1287	arg1	composition					1268:1278	a gum composition	1262:1278	a gum composition that has comparable mechanical properties to commercially procured chewing gums	1262:1358	Fitted models were then used to predict a gum composition that has comparable mechanical properties to commercially procured chewing gums.					
30347275	8	58	contain	has	1285:1287	arg2	properties					1311:1320	comparable mechanical properties	1289:1320	comparable mechanical properties	1289:1320	Fitted models were then used to predict a gum composition that has comparable mechanical properties to commercially procured chewing gums.					
30347275	8	59	used	used	1246:1249	arg2	models					1229:1234	Fitted models	1222:1234	Fitted models	1222:1234	Fitted models were then used to predict a gum composition that has comparable mechanical properties to commercially procured chewing gums.					
30347275	0	60	theme	oppanol®	128:135	arg1	elastomer					142:150	oppanol® B 12 elastomer	128:150	oppanol® B 12 elastomer	128:150	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	0	60	theme	oppanol®	128:135	arg1	gum-base					157:164	a gum-base	155:164	a gum-base	155:164	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	3	61	theme	25-run	602:607	arg1	design					651:656	a 25-run, five-factor, two-level D-Optimal mixture design	600:656	design	651:656	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	62	theme	mixture	643:649	arg1	design					651:656	a 25-run, five-factor, two-level D-Optimal mixture design	600:656	design	651:656	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	11	63	theme	MCGs	1721:1724	arg1	quality					1706:1712	the quality	1702:1712	the quality of the MCGs	1702:1724	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	3	64	theme	two-level	623:631	arg1	design					651:656	a 25-run, five-factor, two-level D-Optimal mixture design	600:656	design	651:656	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	10	65	theme	drug	1628:1631	arg1	release					1633:1639	drug release	1628:1639	drug release	1628:1639	Although no differences in mechanical properties were observed, substituting CUR with the inclusion complex was found to significantly enhance drug release.					
30347275	6	66	used	used	1035:1038	arg2	responses					1020:1028	Observed responses	1011:1028	Observed responses	1011:1028	Observed responses were used to generate polynomial models correlating the independent with the dependent variables.					
30347275	8	67	theme	gum	1264:1266	arg1	composition					1268:1278	a gum composition	1262:1278	a gum composition that has comparable mechanical properties to commercially procured chewing gums	1262:1358	Fitted models were then used to predict a gum composition that has comparable mechanical properties to commercially procured chewing gums.					
30347275	11	68	dep	50	1796:1797	arg1	to					1793:1794	to	1793:1794	to	1793:1794	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	10	69	with	CUR	1562:1564	arg1	complex					1585:1591	the inclusion complex	1571:1591	the inclusion complex	1571:1591	Although no differences in mechanical properties were observed, substituting CUR with the inclusion complex was found to significantly enhance drug release.					
30347275	8	70	theme	comparable	1289:1298	arg1	properties					1311:1320	comparable mechanical properties	1289:1320	comparable mechanical properties	1289:1320	Fitted models were then used to predict a gum composition that has comparable mechanical properties to commercially procured chewing gums.					
30347275	0	71	theme	curcumin	100:107	arg1	gums					117:120	curcumin chewing gums	100:120	curcumin chewing gums using oppanol® B 12 elastomer as a gum-base	100:164	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	3	72	theme	polyvinyl	494:502	arg1	polyisobutene					474:486	(X1) polyisobutene	469:486	(X1) polyisobutene	469:486	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	3	72	theme	polyvinyl	494:502	arg1	acetate					504:510	(X2) polyvinyl acetate	489:510	(X2) polyvinyl acetate	489:510	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	11	73	from	impact	1669:1674	arg1	quality					1706:1712	the quality	1702:1712	the quality of the MCGs	1702:1724	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	0	74	theme	gums	117:120	arg1	development					38:48	Formulation development	26:48	Formulation development	26:48	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	0	74	theme	gums	117:120	arg1	characterization					62:77	mechanical characterization	51:77	mechanical characterization	51:77	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	0	74	theme	gums	117:120	arg1	optimization					84:95	optimization	84:95	optimization	84:95	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	2	75	theme	CUR	347:349	arg1	loading					351:357	high CUR loading	342:357	high CUR loading	342:357	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	0	76	theme	mixture	10:16	arg1	design					18:23	D-optimal mixture design	0:23	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.	0:165	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	5	77	theme	uniaxial	792:799	arg1	tests					809:813	uniaxial tension tests	792:813	uniaxial tension tests	792:813	They were characterized by a two-bites texture and uniaxial tension tests to generate force-displacement curves from which the cohesiveness (Y1), springiness (Y2), chewiness (Y3), compressibility (Y4), resistance to extension (Y5), and extensibility (Y6) were measured.					
30347275	8	78	theme	chewing	1347:1353	arg1	gums					1355:1358	commercially procured chewing gums	1325:1358	commercially procured chewing gums	1325:1358	Fitted models were then used to predict a gum composition that has comparable mechanical properties to commercially procured chewing gums.					
30347275	11	79	theme	gum	1684:1686	arg1	ingredient					1688:1697	each gum ingredient	1679:1697	each gum ingredient	1679:1697	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	2	80	theme	mastication	373:383	arg1	properties					385:394	desirable mastication properties	363:394	desirable mastication properties	363:394	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	11	81	with	gums	1780:1783	arg1	loading					1805:1811	up to 50% drug loading	1790:1811	up to 50% drug loading	1790:1811	This study highlighted the impact of each gum ingredient on the quality of the MCGs and demonstrated the feasibility of preparing chewing gums with up to 50% drug loading.					
30347275	0	82	theme	mechanical	51:60	arg1	characterization					62:77	mechanical characterization	51:77	mechanical characterization	51:77	D-optimal mixture design: Formulation development, mechanical characterization, and optimization of curcumin chewing gums using oppanol® B 12 elastomer as a gum-base.					
30347275	1	83	contain	have	195:198	arg1	gums					190:193	Curcumin (CUR) chewing gums	167:193	Curcumin (CUR) chewing gums	167:193	Curcumin (CUR) chewing gums have potential therapeutic benefits to head and neck cancer patients.					
30347275	1	83	contain	have	195:198	arg2	benefits					222:229	potential therapeutic benefits	200:229	potential therapeutic benefits	200:229	Curcumin (CUR) chewing gums have potential therapeutic benefits to head and neck cancer patients.					
30347275	2	84	theme	medicated	307:315	arg1	MCGs					331:334	MCGs	331:334	MCGs	331:334	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	2	84	theme	medicated	307:315	arg1	gums					325:328	medicated chewing gums	307:328	medicated chewing gums (MCGs) with high CUR loading	307:357	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	2	85	with	gums	325:328	arg1	loading					351:357	high CUR loading	342:357	high CUR loading	342:357	The objective of this work was to develop medicated chewing gums (MCGs) with high CUR loading and desirable mastication properties.					
30347275	1	86	theme	therapeutic	210:220	arg1	benefits					222:229	potential therapeutic benefits	200:229	potential therapeutic benefits	200:229	Curcumin (CUR) chewing gums have potential therapeutic benefits to head and neck cancer patients.					
30347275	9	87	theme	CUR	1409:1411	arg1	complex					1439:1445	CUR/SBE-β-CD inclusion complex	1416:1445	CUR/SBE-β-CD inclusion complex	1416:1445	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	9	87	theme	CUR	1409:1411	arg1	%					1397:1397	50%	1395:1397	50% of either CUR or CUR/SBE-β-CD inclusion complex	1395:1445	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	9	87	theme	CUR	1409:1411	arg1	CUR					1409:1411	CUR	1409:1411	CUR	1409:1411	The optimized MCG was loaded with 50% of either CUR or CUR/SBE-β-CD inclusion complex and tested in vitro for drug release.					
30347275	3	88	theme	mechanical	560:569	arg1	properties					571:580	the mechanical properties	556:580	the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design	556:656	This was accomplished by evaluating the effect of five gum ingredients: (X1) polyisobutene, (X2) polyvinyl acetate, (X3) wood rosin, (X4) wax, and (X5) CUR on the mechanical properties of the MCGs using a 25-run, five-factor, two-level D-Optimal mixture design.					
30347275	4	89	theme	CUR	659:661	arg1	MCGs					663:666	CUR MCGs	659:666	CUR MCGs	659:666	CUR MCGs were prepared by the conventional fusion method for making chewing gums.					
30539396	0	0	theme	graft	73:77	arg1	polymerization					79:92	graft polymerization	73:92	graft polymerization	73:92	Preparation of ion exchange resin using soluble starch and acrylamide by graft polymerization and hydrolysis.					
30539396	3	1	theme	ion	662:664	arg1	process					675:681	the ion exchange process	658:681	the ion exchange process	658:681	During the determination of heavy metal adsorption, the Na+ concentration diffused by SR-16 into the solution was also analysed to investigate the ion exchange process.					
30539396	4	2	contain	has	819:821	arg2	capacity					847:854	an excellent adsorption capacity	823:854	an excellent adsorption capacity to the removal of heavy metal pollution	823:894	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	2	contain	has	819:821	arg1	SR-16					813:817	SR-16	813:817	SR-16	813:817	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	1	3	theme	exchange	240:247	arg1	resin					249:253	a low-cost ion exchange resin	225:253	a low-cost ion exchange resin	225:253	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	1	4	theme	Ni2+	319:322	arg1	ions					302:305	the heavy metal ions	286:305	the heavy metal ions of Cr3+ and Ni2+	286:322	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	4	5	with	progress	966:973	arg1	-COONa					980:985	-COONa	980:985	-COONa attached on the network structure	980:1019	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	6	theme	metal	880:884	arg1	pollution					886:894	heavy metal pollution	874:894	heavy metal pollution	874:894	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	7	theme	heavy	874:878	arg1	pollution					886:894	heavy metal pollution	874:894	heavy metal pollution	874:894	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	8	theme	SR-16	718:722	arg1	morphology					704:713	morphology	704:713	morphology	704:713	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	8	theme	SR-16	718:722	arg1	composition					688:698	composition	688:698	composition	688:698	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	1	9	theme	graft	163:167	arg1	polymerization					169:182	graft polymerization	163:182	graft polymerization	163:182	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	4	10	theme	pollution	886:894	arg1	removal					863:869	the removal	859:869	the removal of heavy metal pollution	859:894	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	3	11	theme	exchange	666:673	arg1	process					675:681	the ion exchange process	658:681	the ion exchange process	658:681	During the determination of heavy metal adsorption, the Na+ concentration diffused by SR-16 into the solution was also analysed to investigate the ion exchange process.					
30539396	4	12	theme	ion	953:955	arg1	progress					966:973	ion exchange progress	953:973	ion exchange progress with -COONa attached on the network structure	953:1019	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	13	attach	attached	987:994	arg3	structure					1011:1019	the network structure	999:1019	the network structure	999:1019	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	13	attach	attached	987:994	arg2	-COONa					980:985	-COONa	980:985	-COONa attached on the network structure	980:1019	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	14	theme	adsorption	901:910	arg1	mechanism					912:920	the adsorption mechanism	897:920	the adsorption mechanism of SR-16	897:929	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	2	15	theme	adsorption	403:412	arg1	experiments					414:424	the adsorption experiments	399:424	the adsorption experiments	399:424	The hydrolysis progresses by adding NaOH to convert -CONH2 to -COONa, and the adsorption experiments confirmed that the functional group to adsorb heavy metals is -COO-, rather than -CONH2.					
30539396	1	16	theme	aqueous	187:193	arg1	solution					195:202	aqueous solution	187:202	aqueous solution	187:202	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	0	17	theme	exchange	19:26	arg1	resin					28:32	ion exchange resin	15:32	ion exchange resin	15:32	Preparation of ion exchange resin using soluble starch and acrylamide by graft polymerization and hydrolysis.					
30539396	0	18	theme	ion	15:17	arg1	resin					28:32	ion exchange resin	15:32	ion exchange resin	15:32	Preparation of ion exchange resin using soluble starch and acrylamide by graft polymerization and hydrolysis.					
30539396	2	19	theme	functional	445:454	arg1	group					456:460	the functional group	441:460	the functional group to adsorb heavy metals	441:483	The hydrolysis progresses by adding NaOH to convert -CONH2 to -COONa, and the adsorption experiments confirmed that the functional group to adsorb heavy metals is -COO-, rather than -CONH2.					
30539396	2	19	theme	functional	445:454	arg1	-COO-					488:492	-COO-	488:492	-COO-	488:492	The hydrolysis progresses by adding NaOH to convert -CONH2 to -COONa, and the adsorption experiments confirmed that the functional group to adsorb heavy metals is -COO-, rather than -CONH2.					
30539396	3	20	theme	adsorption	555:564	arg1	determination					526:538	the determination	522:538	the determination of heavy metal adsorption	522:564	During the determination of heavy metal adsorption, the Na+ concentration diffused by SR-16 into the solution was also analysed to investigate the ion exchange process.					
30539396	1	21	theme	hydrolysis	208:217	arg1	step					219:222	hydrolysis step	208:222	hydrolysis step	208:222	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	0	22	theme	resin	28:32	arg1	Preparation					0:10	Preparation	0:10	Preparation of ion exchange resin using soluble starch and acrylamide by graft polymerization and hydrolysis.	0:108	Preparation of ion exchange resin using soluble starch and acrylamide by graft polymerization and hydrolysis.					
30539396	1	23	theme	heavy	290:294	arg1	ions					302:305	the heavy metal ions	286:305	the heavy metal ions of Cr3+ and Ni2+	286:322	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	4	24	theme	SR-16	925:929	arg1	mechanism					912:920	the adsorption mechanism	897:920	the adsorption mechanism of SR-16	897:929	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	25	theme	elemental	757:765	arg1	analyser					767:774	elemental analyser	757:774	elemental analyser	757:774	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	1	26	theme	metal	296:300	arg1	ions					302:305	the heavy metal ions	286:305	the heavy metal ions of Cr3+ and Ni2+	286:322	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	4	27	theme	excellent	826:834	arg1	capacity					847:854	an excellent adsorption capacity	823:854	an excellent adsorption capacity to the removal of heavy metal pollution	823:894	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	3	28	theme	heavy	543:547	arg1	adsorption					555:564	heavy metal adsorption	543:564	heavy metal adsorption	543:564	During the determination of heavy metal adsorption, the Na+ concentration diffused by SR-16 into the solution was also analysed to investigate the ion exchange process.					
30539396	0	29	theme	soluble	40:46	arg1	starch					48:53	soluble starch	40:53	soluble starch	40:53	Preparation of ion exchange resin using soluble starch and acrylamide by graft polymerization and hydrolysis.					
30539396	3	30	theme	metal	549:553	arg1	adsorption					555:564	heavy metal adsorption	543:564	heavy metal adsorption	543:564	During the determination of heavy metal adsorption, the Na+ concentration diffused by SR-16 into the solution was also analysed to investigate the ion exchange process.					
30539396	4	31	theme	network	1003:1009	arg1	structure					1011:1019	the network structure	999:1019	the network structure	999:1019	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	32	theme	exchange	957:964	arg1	progress					966:973	ion exchange progress	953:973	ion exchange progress with -COONa attached on the network structure	953:1019	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	1	33	theme	soluble	119:125	arg1	starch					127:132	soluble starch	119:132	soluble starch	119:132	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	4	34	theme	adsorption	836:845	arg1	capacity					847:854	an excellent adsorption capacity	823:854	an excellent adsorption capacity to the removal of heavy metal pollution	823:894	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	4	35	dep	composition	688:698	arg1	The					684:686	The	684:686	The	684:686	The composition and morphology of SR-16 was characterized by FT-IR, SEM, elemental analyser and EDS, and the results showed that SR-16 has an excellent adsorption capacity to the removal of heavy metal pollution; the adsorption mechanism of SR-16 could be explained by ion exchange progress with -COONa attached on the network structure.					
30539396	2	36	theme	heavy	472:476	arg1	metals					478:483	heavy metals	472:483	heavy metals	472:483	The hydrolysis progresses by adding NaOH to convert -CONH2 to -COONa, and the adsorption experiments confirmed that the functional group to adsorb heavy metals is -COO-, rather than -CONH2.					
30539396	1	37	theme	low-cost	227:234	arg1	resin					249:253	a low-cost ion exchange resin	225:253	a low-cost ion exchange resin	225:253	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	1	38	theme	Cr3+	310:313	arg1	ions					302:305	the heavy metal ions	286:305	the heavy metal ions of Cr3+ and Ni2+	286:322	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30539396	3	39	theme	Na+	571:573	arg1	concentration					575:587	the Na+ concentration	567:587	the Na+ concentration diffused by SR-16 into the solution	567:623	During the determination of heavy metal adsorption, the Na+ concentration diffused by SR-16 into the solution was also analysed to investigate the ion exchange process.					
30539396	1	40	theme	ion	236:238	arg1	resin					249:253	a low-cost ion exchange resin	225:253	a low-cost ion exchange resin	225:253	Based on soluble starch and acrylamide by performing graft polymerization in aqueous solution and hydrolysis step, a low-cost ion exchange resin has been synthesized to remove the heavy metal ions of Cr3+ and Ni2+.					
30086947	9	0	theme	time	1665:1668	arg1	repeatability					1638:1650	repeatability	1638:1650	repeatability	1638:1650	Furthermore, the column demonstrated satisfactory stability and repeatability of retention time and enantioselectivity using homatropine as model analyte.					
30086947	9	0	theme	time	1665:1668	arg1	stability					1624:1632	satisfactory stability	1611:1632	satisfactory stability	1611:1632	Furthermore, the column demonstrated satisfactory stability and repeatability of retention time and enantioselectivity using homatropine as model analyte.					
30086947	4	1	theme	preparation	687:697	arg1	composition					733:743	composition	733:743	composition	733:743	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	1	theme	preparation	687:697	arg1	conditions					699:708	The preparation conditions	683:708	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS)	683:867	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	1	theme	preparation	687:697	arg1	type					724:727	type	724:727	type	724:727	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	8	2	theme	optimal	1334:1340	arg1	conditions					1342:1351	the optimal conditions	1330:1351	the optimal conditions	1330:1351	Under the optimal conditions, tropicamide, homatropine, homatropine methylbromide, brompheniramine, chlorpheniramine and clorprenaline were all totally separated with the resolution (Rs) values of 2.84, 4.70, 4.61, 3.01, 2.57 and 2.33, respectively.					
30086947	8	3	theme	2.84	1521:1524	arg1	values					1511:1516	the resolution (Rs) values	1491:1516	the resolution (Rs) values	1491:1516	Under the optimal conditions, tropicamide, homatropine, homatropine methylbromide, brompheniramine, chlorpheniramine and clorprenaline were all totally separated with the resolution (Rs) values of 2.84, 4.70, 4.61, 3.01, 2.57 and 2.33, respectively.					
30086947	7	4	theme	basic	1270:1274	arg1	drugs					1276:1280	six basic drugs	1266:1280	six basic drugs	1266:1280	Then, the monolithic column was applied to enantioseparation of six basic drugs in capillary electrochromatography (CEC).					
30086947	0	5	theme	basic	111:115	arg1	drugs					117:121	basic drugs	111:121	basic drugs	111:121	A novel one-pot strategy to prepare β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs.					
30086947	9	6	theme	enantioselectivity	1674:1691	arg1	repeatability					1638:1650	repeatability	1638:1650	repeatability	1638:1650	Furthermore, the column demonstrated satisfactory stability and repeatability of retention time and enantioselectivity using homatropine as model analyte.					
30086947	9	6	theme	enantioselectivity	1674:1691	arg1	stability					1624:1632	satisfactory stability	1611:1632	satisfactory stability	1611:1632	Furthermore, the column demonstrated satisfactory stability and repeatability of retention time and enantioselectivity using homatropine as model analyte.					
30086947	9	7	theme	satisfactory	1611:1622	arg1	stability					1624:1632	satisfactory stability	1611:1632	satisfactory stability	1611:1632	Furthermore, the column demonstrated satisfactory stability and repeatability of retention time and enantioselectivity using homatropine as model analyte.					
30086947	5	8	theme	scanning	976:983	arg1	SEM					1006:1008	SEM	1006:1008	SEM	1006:1008	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	5	8	theme	scanning	976:983	arg1	microscopy					994:1003	scanning electron microscopy	976:1003	scanning electron microscopy (SEM)	976:1009	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	5	9	theme	monolith	942:949	arg1	area					906:909	The specific surface area	885:909	The specific surface area	885:909	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	5	9	theme	monolith	942:949	arg1	morphology					915:924	morphology	915:924	morphology	915:924	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	5	10	theme	electron	985:992	arg1	SEM					1006:1008	SEM	1006:1008	SEM	1006:1008	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	5	10	theme	electron	985:992	arg1	microscopy					994:1003	scanning electron microscopy	976:1003	scanning electron microscopy (SEM)	976:1009	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	4	11	theme	ratio	761:765	arg1	composition					733:743	composition	733:743	composition	733:743	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	11	theme	ratio	761:765	arg1	type					724:727	type	724:727	type	724:727	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	2	12	theme	polymerization	506:519	arg1	system					521:526	the polymerization system	502:526	the polymerization system for the first time	502:545	Diazabicyclo[5.4.0]undec-7-ene (DBU) as a basic catalyst for the ring opening reaction between β-CD and glycidyl methacrylate (GMA) was introduced into the polymerization system for the first time.					
30086947	7	13	theme	drugs	1276:1280	arg1	enantioseparation					1245:1261	enantioseparation	1245:1261	enantioseparation of six basic drugs in capillary electrochromatography (CEC)	1245:1321	Then, the monolithic column was applied to enantioseparation of six basic drugs in capillary electrochromatography (CEC).					
30086947	4	14	theme	monomer	781:787	arg1	ratio					761:765	porogens，the ratio	748:765	porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS)	748:867	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	3	15	dep	reactions	573:581	arg1	methacrylation					602:615	the in situ methacrylation	590:615	two consecutive reactions namely the in situ methacrylation of β-CD	557:623	Thereby, two consecutive reactions namely the in situ methacrylation of β-CD and copolymerization reaction can be achieved in one pot.					
30086947	0	16	theme	drugs	117:121	arg1	enantioseparation					90:106	enantioseparation	90:106	enantioseparation of basic drugs	90:121	A novel one-pot strategy to prepare β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs.					
30086947	4	17	theme	acid	857:860	arg1	AMPS					863:866	AMPS	863:866	AMPS	863:866	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	17	theme	acid	857:860	arg1	acid					857:860	2-acrylamido-2-methyl propane sulfonic acid	818:860	2-acrylamido-2-methyl propane sulfonic acid (AMPS)	818:867	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	17	theme	acid	857:860	arg1	amount					808:813	amount	808:813	amount	808:813	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	17	theme	acid	857:860	arg1	crosslinker					792:802	crosslinker	792:802	crosslinker	792:802	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	18	theme	functional	770:779	arg1	monomer					781:787	functional monomer	770:787	functional monomer	770:787	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	1	19	theme	polymerization	173:186	arg1	mixture					188:194	the polymerization mixture	169:194	the polymerization mixture	169:194	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	0	20	theme	novel	2:6	arg1	strategy					16:23	A novel one-pot strategy	0:23	A novel one-pot strategy	0:23	A novel one-pot strategy to prepare β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs.					
30086947	9	21	theme	model	1714:1718	arg1	analyte					1720:1726	model analyte	1714:1726	model analyte	1714:1726	Furthermore, the column demonstrated satisfactory stability and repeatability of retention time and enantioselectivity using homatropine as model analyte.					
30086947	4	22	dep	type	724:727	arg1	the					720:722	the	720:722	the	720:722	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	6	23	theme	magnetic	1090:1097	arg1	NMR					1110:1112	NMR	1110:1112	NMR	1110:1112	Raman spectroscopy and nuclear magnetic resonance (NMR) spectroscopy confirmed that β-CD was covalently bonded onto the monolith successfully.					
30086947	6	23	theme	magnetic	1090:1097	arg1	resonance					1099:1107	nuclear magnetic resonance	1082:1107	nuclear magnetic resonance (NMR) spectroscopy	1082:1126	Raman spectroscopy and nuclear magnetic resonance (NMR) spectroscopy confirmed that β-CD was covalently bonded onto the monolith successfully.					
30086947	3	24	theme	consecutive	561:571	arg1	reactions					573:581	two consecutive reactions	557:581	two consecutive reactions namely the in situ methacrylation of β-CD	557:623	Thereby, two consecutive reactions namely the in situ methacrylation of β-CD and copolymerization reaction can be achieved in one pot.					
30086947	3	25	dep	in	594:595	arg1	situ					597:600	situ	597:600	situ	597:600	Thereby, two consecutive reactions namely the in situ methacrylation of β-CD and copolymerization reaction can be achieved in one pot.					
30086947	6	26	theme	resonance	1099:1107	arg1	spectroscopy					1115:1126	nuclear magnetic resonance (NMR) spectroscopy	1082:1126	nuclear magnetic resonance (NMR) spectroscopy	1082:1126	Raman spectroscopy and nuclear magnetic resonance (NMR) spectroscopy confirmed that β-CD was covalently bonded onto the monolith successfully.					
30086947	4	27	theme	porogens，the	748:759	arg1	ratio					761:765	porogens，the ratio	748:765	porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS)	748:867	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	5	28	theme	adsorption	1024:1033	arg1	analysis					1035:1042	nitrogen adsorption analysis	1015:1042	nitrogen adsorption analysis	1015:1042	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	2	29	theme	opening	420:426	arg1	reaction					428:435	the ring opening reaction	411:435	the ring opening reaction between β-CD and glycidyl methacrylate (GMA)	411:480	Diazabicyclo[5.4.0]undec-7-ene (DBU) as a basic catalyst for the ring opening reaction between β-CD and glycidyl methacrylate (GMA) was introduced into the polymerization system for the first time.					
30086947	0	30	theme	one-pot	8:14	arg1	strategy					16:23	A novel one-pot strategy	0:23	A novel one-pot strategy	0:23	A novel one-pot strategy to prepare β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs.					
30086947	1	31	theme	β-CD	285:288	arg1	column					342:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	8	32	theme	homatropine	1380:1390	arg1	methylbromide					1392:1404	homatropine methylbromide	1380:1404	homatropine methylbromide	1380:1404	Under the optimal conditions, tropicamide, homatropine, homatropine methylbromide, brompheniramine, chlorpheniramine and clorprenaline were all totally separated with the resolution (Rs) values of 2.84, 4.70, 4.61, 3.01, 2.57 and 2.33, respectively.					
30086947	8	33	theme	resolution	1495:1504	arg1	values					1511:1516	the resolution (Rs) values	1491:1516	the resolution (Rs) values	1491:1516	Under the optimal conditions, tropicamide, homatropine, homatropine methylbromide, brompheniramine, chlorpheniramine and clorprenaline were all totally separated with the resolution (Rs) values of 2.84, 4.70, 4.61, 3.01, 2.57 and 2.33, respectively.					
30086947	2	34	theme	ring	415:418	arg1	reaction					428:435	the ring opening reaction	411:435	the ring opening reaction between β-CD and glycidyl methacrylate (GMA)	411:480	Diazabicyclo[5.4.0]undec-7-ene (DBU) as a basic catalyst for the ring opening reaction between β-CD and glycidyl methacrylate (GMA) was introduced into the polymerization system for the first time.					
30086947	1	35	theme	functionalized	290:303	arg1	column					342:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	2	36	theme	first	536:540	arg1	time					542:545	the first time	532:545	the first time	532:545	Diazabicyclo[5.4.0]undec-7-ene (DBU) as a basic catalyst for the ring opening reaction between β-CD and glycidyl methacrylate (GMA) was introduced into the polymerization system for the first time.					
30086947	6	37	theme	Raman	1059:1063	arg1	spectroscopy					1065:1076	Raman spectroscopy	1059:1076	Raman spectroscopy	1059:1076	Raman spectroscopy and nuclear magnetic resonance (NMR) spectroscopy confirmed that β-CD was covalently bonded onto the monolith successfully.					
30086947	4	38	theme	2-acrylamido-2-methyl	818:838	arg1	AMPS					863:866	AMPS	863:866	AMPS	863:866	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	38	theme	2-acrylamido-2-methyl	818:838	arg1	acid					857:860	2-acrylamido-2-methyl propane sulfonic acid	818:860	2-acrylamido-2-methyl propane sulfonic acid (AMPS)	818:867	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	6	39	theme	nuclear	1082:1088	arg1	NMR					1110:1112	NMR	1110:1112	NMR	1110:1112	Raman spectroscopy and nuclear magnetic resonance (NMR) spectroscopy confirmed that β-CD was covalently bonded onto the monolith successfully.					
30086947	6	39	theme	nuclear	1082:1088	arg1	resonance					1099:1107	nuclear magnetic resonance	1082:1107	nuclear magnetic resonance (NMR) spectroscopy	1082:1126	Raman spectroscopy and nuclear magnetic resonance (NMR) spectroscopy confirmed that β-CD was covalently bonded onto the monolith successfully.					
30086947	1	40	theme	novel	208:212	arg1	strategy					247:254	a novel, convenient and low-cost one-pot strategy	206:254	a novel, convenient and low-cost one-pot strategy	206:254	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	1	41	theme	organic	305:311	arg1	column					342:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	8	42	theme	Rs	1507:1508	arg1	values					1511:1516	the resolution (Rs) values	1491:1516	the resolution (Rs) values	1491:1516	Under the optimal conditions, tropicamide, homatropine, homatropine methylbromide, brompheniramine, chlorpheniramine and clorprenaline were all totally separated with the resolution (Rs) values of 2.84, 4.70, 4.61, 3.01, 2.57 and 2.33, respectively.					
30086947	3	43	theme	in	594:595	arg1	methacrylation					602:615	the in situ methacrylation	590:615	two consecutive reactions namely the in situ methacrylation of β-CD	557:623	Thereby, two consecutive reactions namely the in situ methacrylation of β-CD and copolymerization reaction can be achieved in one pot.					
30086947	7	44	theme	monolithic	1212:1221	arg1	column					1223:1228	the monolithic column	1208:1228	the monolithic column	1208:1228	Then, the monolithic column was applied to enantioseparation of six basic drugs in capillary electrochromatography (CEC).					
30086947	0	45	theme	functionalized	51:64	arg1	monoliths					76:84	β-cyclodextrin functionalized capillary monoliths	36:84	β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs	36:121	A novel one-pot strategy to prepare β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs.					
30086947	1	46	theme	polymer	313:319	arg1	column					342:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	3	47	theme	copolymerization	629:644	arg1	reaction					646:653	copolymerization reaction	629:653	copolymerization reaction	629:653	Thereby, two consecutive reactions namely the in situ methacrylation of β-CD and copolymerization reaction can be achieved in one pot.					
30086947	4	48	theme	sulfonic	848:855	arg1	AMPS					863:866	AMPS	863:866	AMPS	863:866	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	48	theme	sulfonic	848:855	arg1	acid					857:860	2-acrylamido-2-methyl propane sulfonic acid	818:860	2-acrylamido-2-methyl propane sulfonic acid (AMPS)	818:867	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	0	49	theme	β-cyclodextrin	36:49	arg1	monoliths					76:84	β-cyclodextrin functionalized capillary monoliths	36:84	β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs	36:121	A novel one-pot strategy to prepare β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs.					
30086947	1	50	theme	monolithic	321:330	arg1	column					342:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	2	51	theme	glycidyl	454:461	arg1	methacrylate					463:474	glycidyl methacrylate	454:474	glycidyl methacrylate (GMA)	454:480	Diazabicyclo[5.4.0]undec-7-ene (DBU) as a basic catalyst for the ring opening reaction between β-CD and glycidyl methacrylate (GMA) was introduced into the polymerization system for the first time.					
30086947	2	51	theme	glycidyl	454:461	arg1	GMA					477:479	GMA	477:479	GMA	477:479	Diazabicyclo[5.4.0]undec-7-ene (DBU) as a basic catalyst for the ring opening reaction between β-CD and glycidyl methacrylate (GMA) was introduced into the polymerization system for the first time.					
30086947	4	52	theme	propane	840:846	arg1	AMPS					863:866	AMPS	863:866	AMPS	863:866	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	4	52	theme	propane	840:846	arg1	acid					857:860	2-acrylamido-2-methyl propane sulfonic acid	818:860	2-acrylamido-2-methyl propane sulfonic acid (AMPS)	818:867	The preparation conditions including the type and composition of porogens，the ratio of functional monomer to crosslinker and amount of 2-acrylamido-2-methyl propane sulfonic acid (AMPS) were optimized.					
30086947	1	53	theme	native	129:134	arg1	β-cyclodextrin					136:149	native β-cyclodextrin	129:149	native β-cyclodextrin (β-CD)	129:156	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	1	53	theme	native	129:134	arg1	β-CD					152:155	β-CD	152:155	β-CD	152:155	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	1	54	theme	capillary	332:340	arg1	column					342:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	the β-CD functionalized organic polymer monolithic capillary column	281:347	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	9	55	theme	retention	1655:1663	arg1	time					1665:1668	retention time	1655:1668	retention time	1655:1668	Furthermore, the column demonstrated satisfactory stability and repeatability of retention time and enantioselectivity using homatropine as model analyte.					
30086947	7	56	theme	capillary	1285:1293	arg1	electrochromatography					1295:1315	capillary electrochromatography	1285:1315	capillary electrochromatography (CEC)	1285:1321	Then, the monolithic column was applied to enantioseparation of six basic drugs in capillary electrochromatography (CEC).					
30086947	7	56	theme	capillary	1285:1293	arg1	CEC					1318:1320	CEC	1318:1320	CEC	1318:1320	Then, the monolithic column was applied to enantioseparation of six basic drugs in capillary electrochromatography (CEC).					
30086947	1	57	theme	convenient	215:224	arg1	strategy					247:254	a novel, convenient and low-cost one-pot strategy	206:254	a novel, convenient and low-cost one-pot strategy	206:254	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	7	58	from	enantioseparation	1245:1261	arg1	electrochromatography					1295:1315	capillary electrochromatography	1285:1315	capillary electrochromatography (CEC)	1285:1321	Then, the monolithic column was applied to enantioseparation of six basic drugs in capillary electrochromatography (CEC).					
30086947	7	58	from	enantioseparation	1245:1261	arg1	CEC					1318:1320	CEC	1318:1320	CEC	1318:1320	Then, the monolithic column was applied to enantioseparation of six basic drugs in capillary electrochromatography (CEC).					
30086947	3	59	theme	β-CD	620:623	arg1	methacrylation					602:615	the in situ methacrylation	590:615	two consecutive reactions namely the in situ methacrylation of β-CD	557:623	Thereby, two consecutive reactions namely the in situ methacrylation of β-CD and copolymerization reaction can be achieved in one pot.					
30086947	5	60	theme	prepared	933:940	arg1	monolith					942:949	the prepared monolith	929:949	the prepared monolith	929:949	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	5	61	theme	specific	889:896	arg1	area					906:909	The specific surface area	885:909	The specific surface area	885:909	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	2	62	theme	basic	392:396	arg1	catalyst					398:405	a basic catalyst	390:405	a basic catalyst for the ring opening reaction between β-CD and glycidyl methacrylate (GMA)	390:480	Diazabicyclo[5.4.0]undec-7-ene (DBU) as a basic catalyst for the ring opening reaction between β-CD and glycidyl methacrylate (GMA) was introduced into the polymerization system for the first time.					
30086947	1	63	theme	low-cost	230:237	arg1	strategy					247:254	a novel, convenient and low-cost one-pot strategy	206:254	a novel, convenient and low-cost one-pot strategy	206:254	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
30086947	0	64	theme	capillary	66:74	arg1	monoliths					76:84	β-cyclodextrin functionalized capillary monoliths	36:84	β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs	36:121	A novel one-pot strategy to prepare β-cyclodextrin functionalized capillary monoliths for enantioseparation of basic drugs.					
30086947	5	65	theme	nitrogen	1015:1022	arg1	analysis					1035:1042	nitrogen adsorption analysis	1015:1042	nitrogen adsorption analysis	1015:1042	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	5	66	theme	surface	898:904	arg1	area					906:909	The specific surface area	885:909	The specific surface area	885:909	The specific surface area and morphology of the prepared monolith were characterized using scanning electron microscopy (SEM) and nitrogen adsorption analysis, respectively.					
30086947	1	67	theme	one-pot	239:245	arg1	strategy					247:254	a novel, convenient and low-cost one-pot strategy	206:254	a novel, convenient and low-cost one-pot strategy	206:254	With native β-cyclodextrin (β-CD) added into the polymerization mixture directly, a novel, convenient and low-cost one-pot strategy was developed to prepare the β-CD functionalized organic polymer monolithic capillary column.					
31797708	6	0	theme	chitosan	1241:1248	arg1	hydrogel					1250:1257	the chitosan hydrogel	1237:1257	the chitosan hydrogel prepared without harmful chemical crosslinkers	1237:1304	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	6	1	theme	low	1310:1312	arg1	viscosity					1314:1322	low viscosity	1310:1322	low viscosity	1310:1322	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	5	2	theme	*	930:930	arg1	s					931:931	154.958 ± 0.731 g*s	913:931	154.958 ± 0.731 g*s	913:931	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	2	theme	*	930:930	arg1	cohesiveness					899:910	cohesiveness	899:910	the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s)	838:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	6	3	theme	aspect	1416:1421	arg1	characterization					1372:1387	the preclinical characterization	1356:1387	the preclinical characterization	1356:1387	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	1	4	theme	ionic	176:180	arg1	gelation					182:189	ionic gelation	176:189	ionic gelation in lactic acid solution	176:213	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	6	5	theme	key	1465:1467	arg1	importance					1469:1478	key importance	1465:1478	key importance	1465:1478	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	0	6	theme	application	103:113	arg1	characteristics					115:129	suitable application characteristics	94:129	suitable application characteristics	94:129	Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics.					
31797708	5	7	dep	enabled	830:836	arg1	so					1014:1015	so	1014:1015	so	1014:1015	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	6	8	theme	chitosan	1174:1181	arg1	hydrogels					1183:1191	the composite chitosan hydrogels	1160:1191	the composite chitosan hydrogels with ibuprofen	1160:1206	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	4	9	theme	pH	687:688	arg1	values					690:695	pH values	687:695	pH values of the composite hydrogels	687:722	pH values of the composite hydrogels were in the range of 4.80-5.27, thus physiologically acceptable.					
31797708	0	10	theme	suitable	94:101	arg1	characteristics					115:129	suitable application characteristics	94:129	suitable application characteristics	94:129	Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics.					
31797708	6	11	theme	harmful	1276:1282	arg1	crosslinkers					1293:1304	harmful chemical crosslinkers	1276:1304	harmful chemical crosslinkers	1276:1304	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	6	12	theme	composite	1164:1172	arg1	hydrogels					1183:1191	the composite chitosan hydrogels	1160:1191	the composite chitosan hydrogels with ibuprofen	1160:1206	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	2	13	theme	oil	520:522	arg1	16.21-22.56 nm					538:551	16.21-22.56 nm	538:551	16.21-22.56 nm	538:551	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	2	13	theme	oil	520:522	arg1	nanodroplets					524:535	oil nanodroplets	520:535	oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel	520:600	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	4	14	from	acceptable	777:786	arg1	range					736:740	the range	732:740	the range of 4.80-5.27	732:753	pH values of the composite hydrogels were in the range of 4.80-5.27, thus physiologically acceptable.					
31797708	5	15	theme	-12.042 ± 1.161 g	992:1008	arg1	s					1010:1010	-12.042 ± 1.161 g*s	992:1010	-12.042 ± 1.161 g*s	992:1010	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	15	theme	-12.042 ± 1.161 g	992:1008	arg1	adhesiveness					978:989	adhesiveness	978:989	adhesiveness (-12.042 ± 1.161 g*s)	978:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	2	16	theme	electrical	374:383	arg1	measurement					398:408	The electrical conductivity measurement	370:408	The electrical conductivity measurement	370:408	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	3	17	dep	In	603:604	arg1	vitro					606:610	vitro	606:610	vitro	606:610	In vitro ibuprofen release was sustained for 12 h and followed zero-order kinetics.					
31797708	1	18	theme	drug	364:367	arg1	%					333:333	0.25-1%	327:333	0.25-1% of the polymer	327:348	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	1	18	theme	drug	364:367	arg1	polymer					342:348	the polymer	338:348	the polymer	338:348	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	1	18	theme	drug	364:367	arg1	%					355:355	5%	354:355	5% of the drug	354:367	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	1	18	theme	drug	364:367	arg1	drug					364:367	the drug	360:367	the drug	360:367	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	5	19	theme	*	1009:1009	arg1	s					1010:1010	-12.042 ± 1.161 g*s	992:1010	-12.042 ± 1.161 g*s	992:1010	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	19	theme	*	1009:1009	arg1	adhesiveness					978:989	adhesiveness	978:989	adhesiveness (-12.042 ± 1.161 g*s)	978:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	2	20	theme	conductivity	385:396	arg1	measurement					398:408	The electrical conductivity measurement	370:408	The electrical conductivity measurement	370:408	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	5	21	theme	easy	1113:1116	arg1	removal					1118:1124	easy removal	1113:1124	easy removal	1113:1124	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	6	22	theme	characterization	1436:1451	arg1	characterization					1372:1387	the preclinical characterization	1356:1387	the preclinical characterization	1356:1387	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	1	23	theme	lactic	194:199	arg1	acid					201:204	lactic acid	194:204	lactic acid solution	194:213	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	0	24	theme	Composite	0:8	arg1	chitosan					10:17	Composite chitosan	0:17	Composite chitosan	0:17	Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics.					
31797708	5	25	theme	skin	1073:1076	arg1	surface					1078:1084	skin surface	1073:1084	skin surface	1073:1084	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	4	26	theme	4.80-5.27	745:753	arg1	range					736:740	the range	732:740	the range of 4.80-5.27	732:753	pH values of the composite hydrogels were in the range of 4.80-5.27, thus physiologically acceptable.					
31797708	1	27	theme	acid	201:204	arg1	solution					206:213	lactic acid solution	194:213	lactic acid solution	194:213	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	5	28	theme	maximum	842:848	arg1	rate					863:866	the maximum drug release rate	838:866	the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s)	838:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	1	29	theme	composite	302:310	arg1	hydrogels					312:320	composite hydrogels	302:320	composite hydrogels	302:320	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	6	30	theme	preclinical	1360:1370	arg1	characterization					1372:1387	the preclinical characterization	1356:1387	the preclinical characterization	1356:1387	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	5	31	theme	%	819:819	arg1	chitosan					821:828	0.5% chitosan	816:828	0.5% chitosan	816:828	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	32	theme	textural	1017:1024	arg1	properties					1026:1035	textural properties	1017:1035	textural properties	1017:1035	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	33	theme	release	855:861	arg1	rate					863:866	the maximum drug release rate	838:866	the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s)	838:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	0	34	with	dressings	47:55	arg1	release					82:88	sustained ibuprofen release	62:88	sustained ibuprofen release	62:88	Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics.					
31797708	0	34	with	dressings	47:55	arg1	characteristics					115:129	suitable application characteristics	94:129	suitable application characteristics	94:129	Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics.					
31797708	2	35	theme	embedded	554:561	arg1	16.21-22.56 nm					538:551	16.21-22.56 nm	538:551	16.21-22.56 nm	538:551	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	2	35	theme	embedded	554:561	arg1	nanodroplets					524:535	oil nanodroplets	520:535	oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel	520:600	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	3	36	theme	zero-order	666:675	arg1	kinetics					677:684	zero-order kinetics	666:684	zero-order kinetics	666:684	In vitro ibuprofen release was sustained for 12 h and followed zero-order kinetics.					
31797708	4	37	theme	hydrogels	714:722	arg1	values					690:695	pH values	687:695	pH values of the composite hydrogels	687:722	pH values of the composite hydrogels were in the range of 4.80-5.27, thus physiologically acceptable.					
31797708	2	38	theme	correlation	418:428	arg1	PCS					444:446	PCS	444:446	PCS	444:446	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	2	38	theme	correlation	418:428	arg1	spectroscopy					430:441	photon correlation spectroscopy	411:441	photon correlation spectroscopy (PCS)	411:447	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	0	39	theme	wound	41:45	arg1	dressings					47:55	advanced wound dressings	32:55	advanced wound dressings with sustained ibuprofen release and suitable application characteristics	32:129	Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics.					
31797708	5	40	theme	higher	934:939	arg1	s					931:931	154.958 ± 0.731 g*s	913:931	154.958 ± 0.731 g*s	913:931	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	40	theme	higher	934:939	arg1	cohesiveness					899:910	cohesiveness	899:910	the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s)	838:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	3	41	theme	ibuprofen	612:620	arg1	release					622:628	In vitro ibuprofen release	603:628	In vitro ibuprofen release	603:628	In vitro ibuprofen release was sustained for 12 h and followed zero-order kinetics.					
31797708	2	42	theme	photon	411:416	arg1	PCS					444:446	PCS	444:446	PCS	444:446	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	2	42	theme	photon	411:416	arg1	spectroscopy					430:441	photon correlation spectroscopy	411:441	photon correlation spectroscopy (PCS)	411:447	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	0	43	theme	advanced	32:39	arg1	dressings					47:55	advanced wound dressings	32:55	advanced wound dressings with sustained ibuprofen release and suitable application characteristics	32:129	Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics.					
31797708	1	44	theme	0.25-1	327:332	arg1	%					333:333	0.25-1%	327:333	0.25-1% of the polymer	327:348	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	1	44	theme	0.25-1	327:332	arg1	polymer					342:348	the polymer	338:348	the polymer	338:348	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	1	44	theme	0.25-1	327:332	arg1	drug					364:367	the drug	360:367	the drug	360:367	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	5	45	theme	0.5	816:818	arg1	%					819:819	%	819:819	%	819:819	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	6	46	dep	hydrogel	1250:1257	arg1	microemulsion					1337:1349	oil-in-water microemulsion	1324:1349	oil-in-water microemulsion	1324:1349	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	5	47	theme	239.25 μgh-1cm-2	871:886	arg1	s					931:931	154.958 ± 0.731 g*s	913:931	154.958 ± 0.731 g*s	913:931	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	47	theme	239.25 μgh-1cm-2	871:886	arg1	rate					863:866	the maximum drug release rate	838:866	the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s)	838:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	47	theme	239.25 μgh-1cm-2	871:886	arg1	cohesiveness					899:910	cohesiveness	899:910	the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s)	838:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	2	48	theme	rheological	454:464	arg1	analysis					466:473	rheological analysis	454:473	rheological analysis	454:473	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	6	49	theme	biopharmaceutical	1398:1414	arg1	aspect					1416:1421	their biopharmaceutical aspect	1392:1421	their biopharmaceutical aspect	1392:1421	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	2	50	theme	composite	491:499	arg1	hydrogels					501:509	the composite hydrogels	487:509	the composite hydrogels	487:509	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	3	51	theme	In	603:604	arg1	release					622:628	In vitro ibuprofen release	603:628	In vitro ibuprofen release	603:628	In vitro ibuprofen release was sustained for 12 h and followed zero-order kinetics.					
31797708	6	52	theme	oil-in-water	1324:1335	arg1	microemulsion					1337:1349	oil-in-water microemulsion	1324:1349	oil-in-water microemulsion	1324:1349	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	6	53	theme	first	1139:1143	arg1	study					1145:1149	the first study	1135:1149	the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed	1135:1532	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	6	53	theme	first	1139:1143	arg1	This					1127:1130	This	1127:1130	This	1127:1130	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	1	54	theme	physical	136:143	arg1	hydrogel					154:161	The physical chitosan hydrogel	132:161	The physical chitosan hydrogel	132:161	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	2	55	theme	chitosan	584:591	arg1	hydrogel					593:600	pseudoplastic chitosan hydrogel	570:600	pseudoplastic chitosan hydrogel	570:600	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	1	56	theme	chitosan	145:152	arg1	hydrogel					154:161	The physical chitosan hydrogel	132:161	The physical chitosan hydrogel	132:161	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	4	57	from	range	736:740	arg1	acceptable					777:786	acceptable	777:786	acceptable	777:786	pH values of the composite hydrogels were in the range of 4.80-5.27, thus physiologically acceptable.					
31797708	2	58	theme	pseudoplastic	570:582	arg1	hydrogel					593:600	pseudoplastic chitosan hydrogel	570:600	pseudoplastic chitosan hydrogel	570:600	The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21-22.56 nm) embedded within pseudoplastic chitosan hydrogel.					
31797708	6	59	with	hydrogels	1183:1191	arg1	ibuprofen					1198:1206	ibuprofen	1198:1206	ibuprofen	1198:1206	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	1	60	theme	biocompatible	234:246	arg1	microemulsion					261:273	biocompatible oil-in-water microemulsion	234:273	biocompatible oil-in-water microemulsion with ibuprofen	234:288	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	0	61	theme	ibuprofen	72:80	arg1	release					82:88	sustained ibuprofen release	62:88	sustained ibuprofen release	62:88	Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics.					
31797708	5	62	contain	containing	805:814	arg2	chitosan					821:828	0.5% chitosan	816:828	0.5% chitosan	816:828	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	62	contain	containing	805:814	arg1	formulation					793:803	The formulation	789:803	The formulation containing 0.5% chitosan	789:828	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	6	63	theme	textural	1427:1434	arg1	characterization					1436:1451	textural characterization	1427:1451	textural characterization	1427:1451	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
31797708	1	64	with	microemulsion	261:273	arg1	ibuprofen					280:288	ibuprofen	280:288	ibuprofen	280:288	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	4	65	theme	composite	704:712	arg1	hydrogels					714:722	the composite hydrogels	700:722	the composite hydrogels	700:722	pH values of the composite hydrogels were in the range of 4.80-5.27, thus physiologically acceptable.					
31797708	1	66	theme	oil-in-water	248:259	arg1	microemulsion					261:273	biocompatible oil-in-water microemulsion	234:273	biocompatible oil-in-water microemulsion with ibuprofen	234:288	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	0	67	theme	sustained	62:70	arg1	release					82:88	sustained ibuprofen release	62:88	sustained ibuprofen release	62:88	Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics.					
31797708	1	68	theme	polymer	342:348	arg1	%					333:333	0.25-1%	327:333	0.25-1% of the polymer	327:348	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	1	68	theme	polymer	342:348	arg1	polymer					342:348	the polymer	338:348	the polymer	338:348	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	1	68	theme	polymer	342:348	arg1	%					355:355	5%	354:355	5% of the drug	354:367	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	1	68	theme	polymer	342:348	arg1	drug					364:367	the drug	360:367	the drug	360:367	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	5	69	theme	drug	850:853	arg1	rate					863:866	the maximum drug release rate	838:866	the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s)	838:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	1	70	from	gelation	182:189	arg1	solution					206:213	lactic acid solution	194:213	lactic acid solution	194:213	The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25-1% of the polymer and 5% of the drug.					
31797708	5	71	theme	154.958 ± 0.731 g	913:929	arg1	s					931:931	154.958 ± 0.731 g*s	913:931	154.958 ± 0.731 g*s	913:931	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	5	71	theme	154.958 ± 0.731 g	913:929	arg1	cohesiveness					899:910	cohesiveness	899:910	the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s)	838:1011	The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh-1cm-2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (-12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal.					
31797708	6	72	theme	chemical	1284:1291	arg1	crosslinkers					1293:1304	harmful chemical crosslinkers	1276:1304	harmful chemical crosslinkers	1276:1304	This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient's compliance, were performed.					
30875338	2	0	theme	gadolinium	518:527	arg1	ions					540:543	multiple chelated gadolinium (Gd) (III) ions	500:543	multiple chelated gadolinium (Gd) (III) ions	500:543	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	7	1	theme	in	1128:1129	arg1	experiments					1136:1146	in vitro and in vivo experiments	1115:1146	in vitro and in vivo experiments	1115:1146	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	4	2	theme	Dextran-MBA-polyacrylic	687:709	arg1	NPs					719:721	Dextran-MBA-polyacrylic acid-Gd NPs	687:721	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs	679:721	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	7	3	theme	CONCLUSIONS	1033:1043	arg1	NPs					1077:1079	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs	1033:1079	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs	1033:1079	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	1	4	theme	magnetic	265:272	arg1	resonance					274:282	high-relaxivity T1 magnetic resonance	246:282	high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	246:311	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	0	5	theme	Contrast	95:102	arg1	Agent					104:108	High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent	49:108	High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node	49:144	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	1	6	theme	resonance	274:282	arg1	MRI					293:295	MRI	293:295	MRI	293:295	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	1	6	theme	resonance	274:282	arg1	imaging					284:290	high-relaxivity T1 magnetic resonance imaging	246:290	high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	246:311	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	7	7	theme	accurate	1196:1203	arg1	positioning					1205:1215	accurate positioning	1196:1215	accurate positioning	1196:1215	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	7	8	contain	have	1251:1254	arg1	they					1246:1249	they	1246:1249	they	1246:1249	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	7	8	contain	have	1251:1254	arg2	potential					1262:1270	great potential	1256:1270	great potential for clinical SLN mapping	1256:1295	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	4	9	theme	acid	870:873	arg1	injection					892:900	gadopentetic acid dimeglumine salt injection	857:900	gadopentetic acid dimeglumine salt injection	857:900	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	9	theme	acid	870:873	arg1	eg					853:854	eg	853:854	eg	853:854	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	10	theme	longitudinal	767:778	arg1	r1					792:793	r1	792:793	r1	792:793	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	10	theme	longitudinal	767:778	arg1	relaxivity					780:789	much higher longitudinal relaxivity	755:789	much higher longitudinal relaxivity (r1)	755:794	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	7	11	theme	Dextran-MBA-polyacrylic	1045:1067	arg1	NPs					1077:1079	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs	1033:1079	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs	1033:1079	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	1	12	theme	imaging	284:290	arg1	agent					307:311	high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	246:311	high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	246:311	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	4	13	contain	have	723:726	arg1	RESULTS					679:685	RESULTS	679:685	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs	679:721	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	13	contain	have	723:726	arg2	r1					792:793	r1	792:793	r1	792:793	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	13	contain	have	723:726	arg2	relaxivity					780:789	much higher longitudinal relaxivity	755:789	much higher longitudinal relaxivity (r1)	755:794	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	13	contain	have	723:726	arg2	size					746:749	suitable particle size	728:749	suitable particle size	728:749	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	14	theme	gadopentetic	857:868	arg1	injection					892:900	gadopentetic acid dimeglumine salt injection	857:900	gadopentetic acid dimeglumine salt injection	857:900	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	14	theme	gadopentetic	857:868	arg1	eg					853:854	eg	853:854	eg	853:854	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	15	dep	RESULTS	679:685	arg1	NPs					719:721	Dextran-MBA-polyacrylic acid-Gd NPs	687:721	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs	679:721	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	16	theme	higher	760:765	arg1	r1					792:793	r1	792:793	r1	792:793	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	16	theme	higher	760:765	arg1	relaxivity					780:789	much higher longitudinal relaxivity	755:789	much higher longitudinal relaxivity (r1)	755:794	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	0	17	theme	Sentinel	126:133	arg1	Node					141:144	the Sentinel Lymph Node	122:144	the Sentinel Lymph Node	122:144	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	4	18	dep	agents	845:850	arg1	injection					892:900	gadopentetic acid dimeglumine salt injection	857:900	gadopentetic acid dimeglumine salt injection	857:900	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	18	dep	agents	845:850	arg1	eg					853:854	eg	853:854	eg	853:854	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	19	theme	contrast	836:843	arg1	agents					845:850	commonly used clinical MRI contrast agents	809:850	commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection)	809:901	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	7	20	dep	in	1115:1116	arg1	vitro					1118:1122	vitro	1118:1122	vitro	1118:1122	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	2	21	theme	multiple	500:507	arg1	ions					540:543	multiple chelated gadolinium (Gd) (III) ions	500:543	multiple chelated gadolinium (Gd) (III) ions	500:543	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	2	22	theme	assisted	463:470	arg1	approach					472:479	a self-assembly assisted approach	447:479	a self-assembly assisted approach	447:479	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	5	23	from	SLNs	980:983	arg1	effectiveness					955:967	their effectiveness	949:967	their effectiveness at mapping SLNs	949:983	The in vivo T1-weighted MRI results revealed their effectiveness at mapping SLNs.					
30875338	2	24	theme	Dex-MBA-PAA	405:415	arg1	NPs					418:420	Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs	364:420	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs	356:420	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	0	25	theme	Hybrid	6:11	arg1	Dextran-Gadolinium					13:30	Novel Hybrid Dextran-Gadolinium	0:30	Novel Hybrid Dextran-Gadolinium	0:30	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	2	26	theme	chelated	509:516	arg1	Gd					530:531	Gd	530:531	Gd	530:531	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	2	26	theme	chelated	509:516	arg1	III					535:537	III	535:537	III	535:537	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	2	26	theme	chelated	509:516	arg1	gadolinium					518:527	chelated gadolinium	509:527	multiple chelated gadolinium (Gd) (III) ions	500:543	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	7	27	theme	excellent	1221:1229	arg1	biosafety					1231:1239	excellent biosafety	1221:1239	excellent biosafety	1221:1239	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	7	28	theme	great	1256:1260	arg1	potential					1262:1270	great potential	1256:1270	great potential for clinical SLN mapping	1256:1295	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	5	29	theme	T1-weighted	916:926	arg1	results					932:938	The in vivo T1-weighted MRI results	904:938	The in vivo T1-weighted MRI results	904:938	The in vivo T1-weighted MRI results revealed their effectiveness at mapping SLNs.					
30875338	3	30	from	MRI	624:626	arg1	rats					638:641	Wistar rats	631:641	Wistar rats	631:641	After their characterization was validated, they were used to mapping SLNs by MRI in Wistar rats, and their biosafety was evaluated.					
30875338	1	31	theme	novel	191:195	arg1	dextran-gadolinium					204:221	a novel hybrid dextran-gadolinium	189:221	a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	189:311	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	0	32	theme	Novel	0:4	arg1	Dextran-Gadolinium					13:30	Novel Hybrid Dextran-Gadolinium	0:30	Novel Hybrid Dextran-Gadolinium	0:30	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	4	33	theme	particle	737:744	arg1	size					746:749	suitable particle size	728:749	suitable particle size	728:749	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	1	34	theme	hybrid	197:202	arg1	dextran-gadolinium					204:221	a novel hybrid dextran-gadolinium	189:221	a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	189:311	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	2	35	theme	self-assembly	449:461	arg1	approach					472:479	a self-assembly assisted approach	447:479	a self-assembly assisted approach	447:479	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	4	36	theme	used	818:821	arg1	agents					845:850	commonly used clinical MRI contrast agents	809:850	commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection)	809:901	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	7	37	theme	in	1115:1116	arg1	experiments					1136:1146	in vitro and in vivo experiments	1115:1146	in vitro and in vivo experiments	1115:1146	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	1	38	theme	dextran-gadolinium	204:221	arg1	nanoparticles					223:235	a novel hybrid dextran-gadolinium nanoparticles	189:235	a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	189:311	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	1	38	theme	dextran-gadolinium	204:221	arg1	NPs					238:240	NPs	238:240	NPs	238:240	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	5	39	theme	MRI	928:930	arg1	results					932:938	The in vivo T1-weighted MRI results	904:938	The in vivo T1-weighted MRI results	904:938	The in vivo T1-weighted MRI results revealed their effectiveness at mapping SLNs.					
30875338	1	40	theme	contrast	298:305	arg1	agent					307:311	high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	246:311	high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	246:311	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	0	41	theme	Lymph	135:139	arg1	Node					141:144	the Sentinel Lymph Node	122:144	the Sentinel Lymph Node	122:144	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	1	42	theme	nanoparticles	223:235	arg1	applicability					172:184	the applicability	168:184	the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN)	168:353	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	0	43	theme	High-relaxivity	49:63	arg1	Resonance					77:85	High-relaxivity T1 Magnetic Resonance	49:85	High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node	49:144	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	7	44	theme	SLN	1285:1287	arg1	mapping					1289:1295	clinical SLN mapping	1276:1295	clinical SLN mapping	1276:1295	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	7	45	theme	acid-Gd	1069:1075	arg1	NPs					1077:1079	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs	1033:1079	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs	1033:1079	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	7	46	with	MRI	1187:1189	arg1	positioning					1205:1215	accurate positioning	1196:1215	accurate positioning	1196:1215	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	7	46	with	MRI	1187:1189	arg1	biosafety					1231:1239	excellent biosafety	1221:1239	excellent biosafety	1221:1239	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	2	47	theme	acid	399:402	arg1	NPs					418:420	Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs	364:420	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs	356:420	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	7	48	dep	in	1128:1129	arg1	vivo					1131:1134	vivo	1131:1134	vivo	1131:1134	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	0	49	theme	Magnetic	68:75	arg1	Resonance					77:85	High-relaxivity T1 Magnetic Resonance	49:85	High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node	49:144	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	4	50	theme	salt	887:890	arg1	injection					892:900	gadopentetic acid dimeglumine salt injection	857:900	gadopentetic acid dimeglumine salt injection	857:900	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	50	theme	salt	887:890	arg1	eg					853:854	eg	853:854	eg	853:854	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	3	51	theme	Wistar	631:636	arg1	rats					638:641	Wistar rats	631:641	Wistar rats	631:641	After their characterization was validated, they were used to mapping SLNs by MRI in Wistar rats, and their biosafety was evaluated.					
30875338	4	52	theme	clinical	823:830	arg1	agents					845:850	commonly used clinical MRI contrast agents	809:850	commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection)	809:901	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	7	53	theme	clinical	1276:1283	arg1	mapping					1289:1295	clinical SLN mapping	1276:1295	clinical SLN mapping	1276:1295	CONCLUSIONS Dextran-MBA-polyacrylic acid-Gd NPs were synthesized and validated by in vitro and in vivo experiments for their ability to visualize SLNs by MRI with accurate positioning and excellent biosafety, and they have great potential for clinical SLN mapping.					
30875338	0	54	theme	T1	65:66	arg1	Resonance					77:85	High-relaxivity T1 Magnetic Resonance	49:85	High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node	49:144	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	4	55	theme	dimeglumine	875:885	arg1	injection					892:900	gadopentetic acid dimeglumine salt injection	857:900	gadopentetic acid dimeglumine salt injection	857:900	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	55	theme	dimeglumine	875:885	arg1	eg					853:854	eg	853:854	eg	853:854	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	56	theme	MRI	832:834	arg1	agents					845:850	commonly used clinical MRI contrast agents	809:850	commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection)	809:901	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	4	57	theme	acid-Gd	711:717	arg1	NPs					719:721	Dextran-MBA-polyacrylic acid-Gd NPs	687:721	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs	679:721	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	1	58	theme	sentinel	329:336	arg1	SLN					350:352	SLN	350:352	SLN	350:352	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	1	58	theme	sentinel	329:336	arg1	node					344:347	the sentinel lymph node	325:347	the sentinel lymph node (SLN)	325:353	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	4	59	theme	suitable	728:735	arg1	size					746:749	suitable particle size	728:749	suitable particle size	728:749	RESULTS Dextran-MBA-polyacrylic acid-Gd NPs have suitable particle size and much higher longitudinal relaxivity (r1) than that of commonly used clinical MRI contrast agents (eg, gadopentetic acid dimeglumine salt injection).					
30875338	2	60	dep	METHODS	356:362	arg1	NPs					418:420	Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs	364:420	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs	356:420	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	5	61	dep	in	908:909	arg1	vivo					911:914	vivo	911:914	vivo	911:914	The in vivo T1-weighted MRI results revealed their effectiveness at mapping SLNs.					
30875338	3	62	used	used	600:603	arg2	they					590:593	they	590:593	they	590:593	After their characterization was validated, they were used to mapping SLNs by MRI in Wistar rats, and their biosafety was evaluated.					
30875338	5	63	theme	in	908:909	arg1	results					932:938	The in vivo T1-weighted MRI results	904:938	The in vivo T1-weighted MRI results	904:938	The in vivo T1-weighted MRI results revealed their effectiveness at mapping SLNs.					
30875338	0	64	theme	Imaging	87:93	arg1	Agent					104:108	High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent	49:108	High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node	49:144	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	3	65	theme	mapping	608:614	arg1	SLNs					616:619	mapping SLNs	608:619	mapping SLNs	608:619	After their characterization was validated, they were used to mapping SLNs by MRI in Wistar rats, and their biosafety was evaluated.					
30875338	2	66	theme	Dextran-bis-acrylamide-polyacrylic	364:397	arg1	NPs					418:420	Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs	364:420	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs	356:420	METHODS Dextran-bis-acrylamide-polyacrylic acid (Dex-MBA-PAA) NPs were synthesized through a self-assembly assisted approach and complexed with multiple chelated gadolinium (Gd) (III) ions.					
30875338	1	67	theme	high-relaxivity	246:260	arg1	resonance					274:282	high-relaxivity T1 magnetic resonance	246:282	high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	246:311	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	0	68	theme	Resonance	77:85	arg1	Imaging					87:93	High-relaxivity T1 Magnetic Resonance Imaging	49:93	High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node	49:144	Novel Hybrid Dextran-Gadolinium Nanoparticles as High-relaxivity T1 Magnetic Resonance Imaging Contrast Agent for Mapping the Sentinel Lymph Node.					
30875338	1	69	theme	lymph	338:342	arg1	SLN					350:352	SLN	350:352	SLN	350:352	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	1	69	theme	lymph	338:342	arg1	node					344:347	the sentinel lymph node	325:347	the sentinel lymph node (SLN)	325:353	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30875338	6	70	theme	biological	996:1005	arg1	safety					1007:1012	their biological safety	990:1012	their biological safety	990:1012	And their biological safety was also verified.					
30875338	5	71	theme	mapping	972:978	arg1	SLNs					980:983	mapping SLNs	972:983	mapping SLNs	972:983	The in vivo T1-weighted MRI results revealed their effectiveness at mapping SLNs.					
30875338	1	72	theme	T1	262:263	arg1	resonance					274:282	high-relaxivity T1 magnetic resonance	246:282	high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent	246:311	OBJECTIVES To assess the applicability of a novel hybrid dextran-gadolinium nanoparticles (NPs) as high-relaxivity T1 magnetic resonance imaging (MRI) contrast agent for mapping the sentinel lymph node (SLN).					
30583505	6	0	theme	0.5	1202:1204	arg1	μg					1206:1207	μg	1206:1207	μg	1206:1207	Limits of quantification of 0.5 μg L-1 for estrone and 17β-estradiol, and 1 μg L-1 for testosterone, 17α-ethinylestradiol, progesterone, and estriol were obtained.					
30583505	2	1	theme	phase	614:618	arg1	SPE					632:634	SPE	632:634	SPE	632:634	The developed extraction procedure was based on the solid phase extraction (SPE) technique, but employed hydrogel as the sorbent phase.					
30583505	2	1	theme	phase	614:618	arg1	extraction					620:629	solid phase extraction	608:629	the solid phase extraction (SPE) technique	604:645	The developed extraction procedure was based on the solid phase extraction (SPE) technique, but employed hydrogel as the sorbent phase.					
30583505	1	2	theme	derivatization	530:543	arg1	procedure					545:553	the derivatization procedure	526:553	the derivatization procedure	526:553	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	3	theme	extraction	158:167	arg1	phase					169:173	A new extraction phase	152:173	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin	152:236	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	3	4	theme	extraction	842:851	arg1	efficiency					853:862	the extraction efficiency	838:862	the extraction efficiency	838:862	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	8	5	theme	amphiphilic	1510:1520	arg1	character					1522:1530	an amphiphilic character	1507:1530	an amphiphilic character	1507:1530	Since the sorbent phase has an amphiphilic character, the use of hydrogels is promising for the extraction of medium-to-high polarity compounds.					
30583505	4	6	theme	sorbent	936:942	arg1	Gels					881:884	Gels	881:884	Gels with lower swelling indexes	881:912	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	4	6	theme	sorbent	936:942	arg1	sorbent					936:942	sorbent	936:942	sorbent	936:942	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	4	6	theme	sorbent	936:942	arg1	amounts					925:931	larger amounts	918:931	larger amounts of sorbent	918:942	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	3	7	dep	amount	741:746	arg1	the					737:739	the	737:739	the	737:739	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	2	8	theme	solid	608:612	arg1	SPE					632:634	SPE	632:634	SPE	632:634	The developed extraction procedure was based on the solid phase extraction (SPE) technique, but employed hydrogel as the sorbent phase.					
30583505	2	8	theme	solid	608:612	arg1	extraction					620:629	solid phase extraction	608:629	the solid phase extraction (SPE) technique	604:645	The developed extraction procedure was based on the solid phase extraction (SPE) technique, but employed hydrogel as the sorbent phase.					
30583505	1	9	theme	aqueous	416:422	arg1	samples					424:430	aqueous samples	416:430	aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS)	416:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	10	from	samples	424:430	arg1	extraction					288:297	the extraction	284:297	the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS)	284:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	3	11	theme	flow	805:808	arg1	amount					741:746	amount	741:746	amount	741:746	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	11	theme	flow	805:808	arg1	rate					810:813	flow rate	805:813	flow rate	805:813	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	11	theme	flow	805:808	arg1	parameters					715:724	several parameters	707:724	several parameters	707:724	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	11	theme	flow	805:808	arg1	phase					779:783	the sorbent phase	767:783	the sorbent phase	767:783	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	11	theme	flow	805:808	arg1	composition					752:762	composition	752:762	composition	752:762	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	0	12	theme	Hormones	111:118	arg1	Determination					84:96	Determination	84:96	Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS	84:149	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	1	13	from	extraction	288:297	arg1	samples					424:430	aqueous samples	416:430	aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS)	416:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	3	14	theme	sample	790:795	arg1	amount					741:746	amount	741:746	amount	741:746	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	14	theme	sample	790:795	arg1	volume					797:802	sample volume	790:802	sample volume	790:802	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	14	theme	sample	790:795	arg1	phase					779:783	the sorbent phase	767:783	the sorbent phase	767:783	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	14	theme	sample	790:795	arg1	parameters					715:724	several parameters	707:724	several parameters	707:724	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	14	theme	sample	790:795	arg1	composition					752:762	composition	752:762	composition	752:762	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	0	15	theme	Steroidal	101:109	arg1	Hormones					111:118	Steroidal Hormones	101:118	Steroidal Hormones in Aqueous Samples	101:137	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	3	16	theme	parameters	715:724	arg1	effects					696:702	The effects	692:702	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency,	692:863	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	7	17	theme	110	1373:1375	arg1	%					1368:1368	%	1368:1368	%	1368:1368	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	7	18	theme	0.55	1463:1466	arg1	%					1467:1467	%	1467:1467	%	1467:1467	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	0	19	theme	Aqueous	123:129	arg1	Samples					131:137	Aqueous Samples	123:137	Aqueous Samples	123:137	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	6	20	theme	L-1	1209:1211	arg1	quantification					1184:1197	quantification	1184:1197	quantification of 0.5 μg L-1 for estrone and 17β-estradiol	1184:1241	Limits of quantification of 0.5 μg L-1 for estrone and 17β-estradiol, and 1 μg L-1 for testosterone, 17α-ethinylestradiol, progesterone, and estriol were obtained.					
30583505	1	21	theme	subsequent	437:446	arg1	determination					448:460	subsequent determination	437:460	subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS)	437:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	7	22	theme	80	1366:1367	arg1	%					1368:1368	%	1368:1368	%	1368:1368	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	4	23	theme	extraction	959:968	arg1	yields					970:975	higher extraction yields	952:975	higher extraction yields of analytes	952:987	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	2	24	theme	sorbent	677:683	arg1	phase					685:689	the sorbent phase	673:689	the sorbent phase	673:689	The developed extraction procedure was based on the solid phase extraction (SPE) technique, but employed hydrogel as the sorbent phase.					
30583505	2	24	theme	sorbent	677:683	arg1	hydrogel					661:668	hydrogel	661:668	hydrogel	661:668	The developed extraction procedure was based on the solid phase extraction (SPE) technique, but employed hydrogel as the sorbent phase.					
30583505	4	25	theme	lower	891:895	arg1	indexes					906:912	lower swelling indexes	891:912	lower swelling indexes	891:912	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	4	26	theme	larger	918:923	arg1	sorbent					936:942	sorbent	936:942	sorbent	936:942	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	4	26	theme	larger	918:923	arg1	amounts					925:931	larger amounts	918:931	larger amounts of sorbent	918:942	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	3	27	theme	several	707:713	arg1	pH					786:787	pH	786:787	pH	786:787	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	27	theme	several	707:713	arg1	rate					810:813	flow rate	805:813	flow rate	805:813	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	27	theme	several	707:713	arg1	parameters					715:724	several parameters	707:724	several parameters	707:724	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	27	theme	several	707:713	arg1	composition					752:762	composition	752:762	composition	752:762	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	27	theme	several	707:713	arg1	volume					797:802	sample volume	790:802	sample volume	790:802	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	27	theme	several	707:713	arg1	swelling					824:831	gel swelling	820:831	gel swelling	820:831	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	27	theme	several	707:713	arg1	amount					741:746	amount	741:746	amount	741:746	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	27	theme	several	707:713	arg1	phase					779:783	the sorbent phase	767:783	the sorbent phase	767:783	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	6	28	theme	μg	1206:1207	arg1	L-1					1209:1211	0.5 μg L-1	1202:1211	0.5 μg L-1 for estrone and 17β-estradiol	1202:1241	Limits of quantification of 0.5 μg L-1 for estrone and 17β-estradiol, and 1 μg L-1 for testosterone, 17α-ethinylestradiol, progesterone, and estriol were obtained.					
30583505	8	29	contain	has	1503:1505	arg1	phase					1497:1501	the sorbent phase	1485:1501	the sorbent phase	1485:1501	Since the sorbent phase has an amphiphilic character, the use of hydrogels is promising for the extraction of medium-to-high polarity compounds.					
30583505	8	29	contain	has	1503:1505	arg2	character					1522:1530	an amphiphilic character	1507:1530	an amphiphilic character	1507:1530	Since the sorbent phase has an amphiphilic character, the use of hydrogels is promising for the extraction of medium-to-high polarity compounds.					
30583505	8	30	theme	sorbent	1489:1495	arg1	phase					1497:1501	the sorbent phase	1485:1501	the sorbent phase	1485:1501	Since the sorbent phase has an amphiphilic character, the use of hydrogels is promising for the extraction of medium-to-high polarity compounds.					
30583505	8	31	theme	medium-to-high	1589:1602	arg1	compounds					1613:1621	medium-to-high polarity compounds	1589:1621	medium-to-high polarity compounds	1589:1621	Since the sorbent phase has an amphiphilic character, the use of hydrogels is promising for the extraction of medium-to-high polarity compounds.					
30583505	1	32	theme	hydrogel	184:191	arg1	disks					193:197	hydrogel disks	184:197	hydrogel disks of polyvinyl alcohol (PVOH) and pectin	184:236	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	4	33	theme	analytes	980:987	arg1	yields					970:975	higher extraction yields	952:975	higher extraction yields of analytes	952:987	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	2	34	theme	extraction	620:629	arg1	technique					637:645	the solid phase extraction (SPE) technique	604:645	the solid phase extraction (SPE) technique	604:645	The developed extraction procedure was based on the solid phase extraction (SPE) technique, but employed hydrogel as the sorbent phase.					
30583505	1	35	theme	gas	465:467	arg1	GC-MS/MS					510:517	GC-MS/MS	510:517	GC-MS/MS	510:517	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	35	theme	gas	465:467	arg1	spectrometry					496:507	gas chromatography-tandem mass spectrometry	465:507	gas chromatography-tandem mass spectrometry (GC-MS/MS)	465:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	0	36	theme	Alcohol/Pectin-Based	24:43	arg1	Disks					54:58	Polyvinyl Alcohol/Pectin-Based Hydrogel Disks	14:58	Polyvinyl Alcohol/Pectin-Based Hydrogel Disks	14:58	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	2	37	theme	developed	560:568	arg1	procedure					581:589	The developed extraction procedure	556:589	The developed extraction procedure	556:589	The developed extraction procedure was based on the solid phase extraction (SPE) technique, but employed hydrogel as the sorbent phase.					
30583505	1	38	theme	chromatography-tandem	469:489	arg1	GC-MS/MS					510:517	GC-MS/MS	510:517	GC-MS/MS	510:517	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	38	theme	chromatography-tandem	469:489	arg1	spectrometry					496:507	gas chromatography-tandem mass spectrometry	465:507	gas chromatography-tandem mass spectrometry (GC-MS/MS)	465:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	7	39	theme	Accuracy	1338:1345	arg1	values					1347:1352	Accuracy values	1338:1352	Accuracy values	1338:1352	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	8	40	theme	compounds	1613:1621	arg1	extraction					1575:1584	the extraction	1571:1584	the extraction of medium-to-high polarity compounds	1571:1621	Since the sorbent phase has an amphiphilic character, the use of hydrogels is promising for the extraction of medium-to-high polarity compounds.					
30583505	0	41	theme	Polyvinyl	14:22	arg1	Disks					54:58	Polyvinyl Alcohol/Pectin-Based Hydrogel Disks	14:58	Polyvinyl Alcohol/Pectin-Based Hydrogel Disks	14:58	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	1	42	dep	hormones	316:323	arg1	progesterone					381:392	progesterone	381:392	progesterone	381:392	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	42	dep	hormones	316:323	arg1	estrone					335:341	estrone	335:341	estrone	335:341	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	42	dep	hormones	316:323	arg1	17β-estradiol					344:356	17β-estradiol	344:356	17β-estradiol	344:356	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	42	dep	hormones	316:323	arg1	testosterone					399:410	testosterone	399:410	testosterone	399:410	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	42	dep	hormones	316:323	arg1	hormones					316:323	six steroidal hormones	302:323	six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS)	302:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	42	dep	hormones	316:323	arg1	estriol					326:332	estriol	326:332	estriol	326:332	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	42	dep	hormones	316:323	arg1	17α-ethinylestradiol					359:378	17α-ethinylestradiol	359:378	17α-ethinylestradiol	359:378	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	7	43	dep	%	1467:1467	arg1	to					1469:1470	to	1469:1470	to	1469:1470	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	5	44	theme	PVOH/pectin-based	1021:1037	arg1	hydrogel					1039:1046	the PVOH/pectin-based hydrogel	1017:1046	the PVOH/pectin-based hydrogel as the extraction phase	1017:1070	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	1	45	theme	polyvinyl	202:210	arg1	PVOH					221:224	PVOH	221:224	PVOH	221:224	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	45	theme	polyvinyl	202:210	arg1	alcohol					212:218	polyvinyl alcohol	202:218	polyvinyl alcohol (PVOH)	202:225	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	0	46	theme	Disks	54:58	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.	0:150	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	8	47	theme	polarity	1604:1611	arg1	compounds					1613:1621	medium-to-high polarity compounds	1589:1621	medium-to-high polarity compounds	1589:1621	Since the sorbent phase has an amphiphilic character, the use of hydrogels is promising for the extraction of medium-to-high polarity compounds.					
30583505	4	48	theme	swelling	897:904	arg1	indexes					906:912	lower swelling indexes	891:912	lower swelling indexes	891:912	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	0	49	from	Determination	84:96	arg1	Samples					131:137	Aqueous Samples	123:137	Aqueous Samples	123:137	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	1	50	theme	alcohol	212:218	arg1	disks					193:197	hydrogel disks	184:197	hydrogel disks of polyvinyl alcohol (PVOH) and pectin	184:236	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	0	51	theme	Hydrogel	45:52	arg1	Disks					54:58	Polyvinyl Alcohol/Pectin-Based Hydrogel Disks	14:58	Polyvinyl Alcohol/Pectin-Based Hydrogel Disks	14:58	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	5	52	theme	low-cost	1099:1106	arg1	preparation					1108:1118	low-cost preparation	1099:1118	low-cost preparation	1099:1118	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	4	53	theme	higher	952:957	arg1	yields					970:975	higher extraction yields	952:975	higher extraction yields of analytes	952:987	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	5	54	theme	extraction	1156:1165	arg1	disks					1167:1171	the extraction disks	1152:1171	the extraction disks	1152:1171	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	6	55	theme	quantification	1184:1197	arg1	L-1					1253:1255	1 μg L-1	1248:1255	1 μg L-1 for testosterone, 17α-ethinylestradiol, progesterone, and estriol	1248:1321	Limits of quantification of 0.5 μg L-1 for estrone and 17β-estradiol, and 1 μg L-1 for testosterone, 17α-ethinylestradiol, progesterone, and estriol were obtained.					
30583505	6	55	theme	quantification	1184:1197	arg1	Limits					1174:1179	Limits	1174:1179	Limits of quantification of 0.5 μg L-1 for estrone and 17β-estradiol	1174:1241	Limits of quantification of 0.5 μg L-1 for estrone and 17β-estradiol, and 1 μg L-1 for testosterone, 17α-ethinylestradiol, progesterone, and estriol were obtained.					
30583505	1	56	theme	mass	491:494	arg1	GC-MS/MS					510:517	GC-MS/MS	510:517	GC-MS/MS	510:517	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	56	theme	mass	491:494	arg1	spectrometry					496:507	gas chromatography-tandem mass spectrometry	465:507	gas chromatography-tandem mass spectrometry (GC-MS/MS)	465:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	57	with	samples	424:430	arg1	determination					448:460	subsequent determination	437:460	subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS)	437:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	0	58	theme	Extraction	63:72	arg1	Phase					74:78	Extraction Phase	63:78	Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS	63:149	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	3	59	theme	sorbent	771:777	arg1	phase					779:783	the sorbent phase	767:783	the sorbent phase	767:783	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	5	60	theme	extraction	1055:1064	arg1	phase					1066:1070	the extraction phase	1051:1070	the extraction phase	1051:1070	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	5	61	theme	synthesis	1088:1096	arg1	possibility					1129:1139	the possibility	1125:1139	the possibility of reusing the extraction disks	1125:1171	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	5	61	theme	synthesis	1088:1096	arg1	benefits					999:1006	The main benefits	990:1006	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase	990:1070	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	5	61	theme	synthesis	1088:1096	arg1	preparation					1108:1118	low-cost preparation	1099:1118	low-cost preparation	1099:1118	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	5	61	theme	synthesis	1088:1096	arg1	ease					1080:1083	the ease	1076:1083	the ease of synthesis	1076:1096	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	7	62	theme	%	1427:1427	arg1	%					1436:1436	0.23% to 22.2%	1423:1436	0.23% to 22.2%	1423:1436	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	6	63	theme	μg	1250:1251	arg1	L-1					1253:1255	1 μg L-1	1248:1255	1 μg L-1 for testosterone, 17α-ethinylestradiol, progesterone, and estriol	1248:1321	Limits of quantification of 0.5 μg L-1 for estrone and 17β-estradiol, and 1 μg L-1 for testosterone, 17α-ethinylestradiol, progesterone, and estriol were obtained.					
30583505	5	64	theme	main	994:997	arg1	benefits					999:1006	The main benefits	990:1006	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase	990:1070	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	5	64	theme	main	994:997	arg1	ease					1080:1083	the ease	1076:1083	the ease of synthesis	1076:1096	The main benefits of using the PVOH/pectin-based hydrogel as the extraction phase are the ease of synthesis, low-cost preparation, and the possibility of reusing the extraction disks.					
30583505	7	65	dep	%	1368:1368	arg1	to					1370:1371	to	1370:1371	to	1370:1371	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	1	66	theme	steroidal	306:314	arg1	progesterone					381:392	progesterone	381:392	progesterone	381:392	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	66	theme	steroidal	306:314	arg1	estrone					335:341	estrone	335:341	estrone	335:341	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	66	theme	steroidal	306:314	arg1	17β-estradiol					344:356	17β-estradiol	344:356	17β-estradiol	344:356	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	66	theme	steroidal	306:314	arg1	testosterone					399:410	testosterone	399:410	testosterone	399:410	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	66	theme	steroidal	306:314	arg1	hormones					316:323	six steroidal hormones	302:323	six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS)	302:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	66	theme	steroidal	306:314	arg1	estriol					326:332	estriol	326:332	estriol	326:332	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	66	theme	steroidal	306:314	arg1	17α-ethinylestradiol					359:378	17α-ethinylestradiol	359:378	17α-ethinylestradiol	359:378	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	7	67	theme	12.3	1472:1475	arg1	%					1467:1467	%	1467:1467	%	1467:1467	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	4	68	with	amounts	925:931	arg1	indexes					906:912	lower swelling indexes	891:912	lower swelling indexes	891:912	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	3	69	theme	gel	820:822	arg1	amount					741:746	amount	741:746	amount	741:746	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	69	theme	gel	820:822	arg1	phase					779:783	the sorbent phase	767:783	the sorbent phase	767:783	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	69	theme	gel	820:822	arg1	parameters					715:724	several parameters	707:724	several parameters	707:724	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	69	theme	gel	820:822	arg1	composition					752:762	composition	752:762	composition	752:762	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	69	theme	gel	820:822	arg1	swelling					824:831	gel swelling	820:831	gel swelling	820:831	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	7	70	dep	%	1436:1436	arg1	to					1429:1430	to	1429:1430	to	1429:1430	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	2	71	theme	extraction	570:579	arg1	procedure					581:589	The developed extraction procedure	556:589	The developed extraction procedure	556:589	The developed extraction procedure was based on the solid phase extraction (SPE) technique, but employed hydrogel as the sorbent phase.					
30583505	0	72	from	Hormones	111:118	arg1	Samples					131:137	Aqueous Samples	123:137	Aqueous Samples	123:137	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	8	73	theme	hydrogels	1544:1552	arg1	promising					1557:1565	promising	1557:1565	promising	1557:1565	Since the sorbent phase has an amphiphilic character, the use of hydrogels is promising for the extraction of medium-to-high polarity compounds.					
30583505	8	73	theme	hydrogels	1544:1552	arg1	use					1537:1539	the use	1533:1539	the use of hydrogels	1533:1552	Since the sorbent phase has an amphiphilic character, the use of hydrogels is promising for the extraction of medium-to-high polarity compounds.					
30583505	4	74	with	Gels	881:884	arg1	indexes					906:912	lower swelling indexes	891:912	lower swelling indexes	891:912	Gels with lower swelling indexes and larger amounts of sorbent ensured higher extraction yields of analytes.					
30583505	1	75	dep	proposed	242:249	arg1	evaluated					270:278	evaluated	270:278	evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure	270:553	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	75	dep	proposed	242:249	arg1	characterized					252:264	characterized	252:264	characterized	252:264	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	3	76	theme	phase	779:783	arg1	amount					741:746	amount	741:746	amount	741:746	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	76	theme	phase	779:783	arg1	phase					779:783	the sorbent phase	767:783	the sorbent phase	767:783	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	3	76	theme	phase	779:783	arg1	composition					752:762	composition	752:762	composition	752:762	The effects of several parameters, including the amount and composition of the sorbent phase, pH, sample volume, flow rate, and gel swelling over the extraction efficiency, were evaluated.					
30583505	1	77	from	hormones	316:323	arg1	samples					424:430	aqueous samples	416:430	aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS)	416:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	1	78	theme	hormones	316:323	arg1	extraction					288:297	the extraction	284:297	the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS)	284:518	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	0	79	from	Samples	131:137	arg1	Determination					84:96	Determination	84:96	Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS	84:149	Evaluation of Polyvinyl Alcohol/Pectin-Based Hydrogel Disks as Extraction Phase for Determination of Steroidal Hormones in Aqueous Samples by GC-MS/MS.					
30583505	1	80	theme	new	154:156	arg1	phase					169:173	A new extraction phase	152:173	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin	152:236	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
30583505	7	81	theme	inter-assay	1389:1399	arg1	precision					1401:1409	the inter-assay precision	1385:1409	the inter-assay precision	1385:1409	Accuracy values ranged from 80% to 110%, while the inter-assay precision ranged from 0.23% to 22.2% and the intra-assay from 0.55% to 12.3%.					
30583505	1	82	theme	pectin	231:236	arg1	disks					193:197	hydrogel disks	184:197	hydrogel disks of polyvinyl alcohol (PVOH) and pectin	184:236	A new extraction phase based on hydrogel disks of polyvinyl alcohol (PVOH) and pectin was proposed, characterized and evaluated for the extraction of six steroidal hormones (estriol, estrone, 17β-estradiol, 17α-ethinylestradiol, progesterone, and testosterone) in aqueous samples with subsequent determination by gas chromatography-tandem mass spectrometry (GC-MS/MS) after the derivatization procedure.					
29525166	6	0	theme	unique	871:876	arg1	drug					918:921	long time period drug	901:921	long time period drug releasing property, antibacterial and mechanical properties	901:981	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	6	0	theme	unique	871:876	arg1	responsiveness					885:898	the unique pH/NIR responsiveness	867:898	the unique pH/NIR responsiveness	867:898	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	3	1	theme	calcium	491:497	arg1	ion					499:501	calcium ion	491:501	calcium ion	491:501	Polydopamine (PDA) was introduced into TOCNFs network to fabricate a PDA/TOCNFs hydrogel through ion-crosslinking with calcium ion as a crosslinker.					
29525166	6	2	theme	mechanical	961:970	arg1	properties					972:981	mechanical properties	961:981	mechanical properties	961:981	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	0	3	theme	drug	97:100	arg1	delivery					102:109	drug delivery	97:109	drug delivery	97:109	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.					
29525166	6	4	theme	new	1101:1103	arg1	vehicle					1119:1125	a new drug delivery vehicle	1099:1125	a new drug delivery vehicle	1099:1125	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	0	5	theme	wound	115:119	arg1	healing					121:127	wound healing	115:127	wound healing	115:127	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.					
29525166	0	6	link	crosslinked	13:23	arg1	hydrogel					52:59	A physically crosslinked polydopamine/nanocellulose hydrogel	0:59	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.	0:128	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.					
29525166	6	7	theme	safe	1148:1151	arg1	alternatives					1153:1164	simple and safe alternatives	1137:1164	simple and safe alternatives to the conventional systems based on the petroleum-based polymers	1137:1230	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	6	8	theme	drug	1105:1108	arg1	vehicle					1119:1125	a new drug delivery vehicle	1099:1125	a new drug delivery vehicle	1099:1125	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	3	9	theme	TOCNFs	411:416	arg1	network					418:424	TOCNFs network	411:424	TOCNFs network to fabricate a PDA/TOCNFs hydrogel through ion-crosslinking with calcium ion as a crosslinker	411:518	Polydopamine (PDA) was introduced into TOCNFs network to fabricate a PDA/TOCNFs hydrogel through ion-crosslinking with calcium ion as a crosslinker.					
29525166	6	10	theme	simple	1137:1142	arg1	alternatives					1153:1164	simple and safe alternatives	1137:1164	simple and safe alternatives to the conventional systems based on the petroleum-based polymers	1137:1230	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	6	11	theme	prepared	1047:1054	arg1	hydrogel					1067:1074	the prepared PDA/TOCNFs hydrogel	1043:1074	the prepared PDA/TOCNFs hydrogel	1043:1074	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	6	11	theme	prepared	1047:1054	arg1	promising					1086:1094	promising	1086:1094	promising	1086:1094	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	5	12	theme	skin	720:723	arg1	experiments					732:742	the in vivo skin defect experiments	708:742	the in vivo skin defect experiments	708:742	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	5	13	theme	wound	832:836	arg1	healing					838:844	wound healing	832:844	wound healing	832:844	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	5	14	theme	resultant	765:773	arg1	hydrogel					785:792	the resultant composite hydrogel	761:792	the resultant composite hydrogel	761:792	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	4	15	from	hydrogels	615:623	arg1	fashion					641:647	an on-demand fashion	628:647	an on-demand fashion	628:647	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	2	16	theme	oxidation	354:362	arg1	method					364:369	2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation method	297:369	2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation method	297:369	Cellulose nanofibrils (TOCNFs) were produced by 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation method.					
29525166	0	17	theme	polydopamine/nanocellulose	25:50	arg1	hydrogel					52:59	A physically crosslinked polydopamine/nanocellulose hydrogel	0:59	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.	0:128	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.					
29525166	4	18	theme	tetracycline	527:538	arg1	hydrochloride					540:552	tetracycline hydrochloride	527:552	tetracycline hydrochloride (TH) which loaded on PDA	527:577	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	4	18	theme	tetracycline	527:538	arg1	TH					555:556	TH	555:556	TH	555:556	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	5	19	contain	had	794:796	arg2	effect					812:817	a synergistic effect	798:817	a synergistic effect	798:817	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	5	19	contain	had	794:796	arg1	hydrogel					785:792	the resultant composite hydrogel	761:792	the resultant composite hydrogel	761:792	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	0	20	theme	crosslinked	13:23	arg1	hydrogel					52:59	A physically crosslinked polydopamine/nanocellulose hydrogel	0:59	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.	0:128	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.					
29525166	2	21	theme	-mediated	344:352	arg1	method					364:369	2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation method	297:369	2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation method	297:369	Cellulose nanofibrils (TOCNFs) were produced by 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation method.					
29525166	6	22	theme	delivery	1110:1117	arg1	vehicle					1119:1125	a new drug delivery vehicle	1099:1125	a new drug delivery vehicle	1099:1125	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	3	23	theme	PDA/TOCNFs	441:450	arg1	hydrogel					452:459	a PDA/TOCNFs hydrogel	439:459	a PDA/TOCNFs hydrogel	439:459	Polydopamine (PDA) was introduced into TOCNFs network to fabricate a PDA/TOCNFs hydrogel through ion-crosslinking with calcium ion as a crosslinker.					
29525166	5	24	theme	defect	725:730	arg1	experiments					732:742	the in vivo skin defect experiments	708:742	the in vivo skin defect experiments	708:742	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	6	25	theme	period	911:916	arg1	drug					918:921	long time period drug	901:921	long time period drug releasing property, antibacterial and mechanical properties	901:981	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	6	25	theme	period	911:916	arg1	responsiveness					885:898	the unique pH/NIR responsiveness	867:898	the unique pH/NIR responsiveness	867:898	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	5	26	theme	synergistic	800:810	arg1	effect					812:817	a synergistic effect	798:817	a synergistic effect	798:817	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	6	27	theme	petroleum-based	1207:1221	arg1	polymers					1223:1230	the petroleum-based polymers	1203:1230	the petroleum-based polymers	1203:1230	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	1	28	theme	-responsive	181:191	arg1	hydrogel					193:200	a pH/near-infrared (NIR)-responsive hydrogel	157:200	a pH/near-infrared (NIR)-responsive hydrogel for on-demand drug delivery and wound healing	157:246	In this study, we reported a pH/near-infrared (NIR)-responsive hydrogel for on-demand drug delivery and wound healing.					
29525166	1	29	theme	on-demand	206:214	arg1	delivery					221:228	on-demand drug delivery	206:228	on-demand drug delivery	206:228	In this study, we reported a pH/near-infrared (NIR)-responsive hydrogel for on-demand drug delivery and wound healing.					
29525166	6	30	theme	PDA/TOCNFs	1056:1065	arg1	hydrogel					1067:1074	the prepared PDA/TOCNFs hydrogel	1043:1074	the prepared PDA/TOCNFs hydrogel	1043:1074	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	6	30	theme	PDA/TOCNFs	1056:1065	arg1	promising					1086:1094	promising	1086:1094	promising	1086:1094	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	6	31	theme	time	906:909	arg1	period					911:916	long time period	901:916	long time period drug releasing property, antibacterial and mechanical properties	901:981	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	5	32	dep	in	712:713	arg1	vivo					715:718	vivo	715:718	vivo	715:718	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	5	33	theme	composite	775:783	arg1	hydrogel					785:792	the resultant composite hydrogel	761:792	the resultant composite hydrogel	761:792	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	4	34	theme	prepared	606:613	arg1	hydrogels					615:623	the prepared hydrogels	602:623	the prepared hydrogels in an on-demand fashion	602:647	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	0	35	theme	versatile	74:82	arg1	vehicles					84:91	potential versatile vehicles	64:91	potential versatile vehicles for drug delivery and wound healing	64:127	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.					
29525166	6	36	theme	long	901:904	arg1	period					911:916	long time period	901:916	long time period drug releasing property, antibacterial and mechanical properties	901:981	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	4	37	theme	lower	674:678	arg1	conditions					683:692	lower pH conditions	674:692	lower pH conditions	674:692	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	0	38	theme	potential	64:72	arg1	vehicles					84:91	potential versatile vehicles	64:91	potential versatile vehicles for drug delivery and wound healing	64:127	A physically crosslinked polydopamine/nanocellulose hydrogel as potential versatile vehicles for drug delivery and wound healing.					
29525166	4	39	attach	released	588:595	arg2	TH					555:556	TH	555:556	TH	555:556	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	4	39	attach	released	588:595	arg2	hydrochloride					540:552	tetracycline hydrochloride	527:552	tetracycline hydrochloride (TH) which loaded on PDA	527:577	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	4	39	attach	released	588:595	arg1	hydrogels					615:623	the prepared hydrogels	602:623	the prepared hydrogels in an on-demand fashion	602:647	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	4	40	theme	on-demand	631:639	arg1	fashion					641:647	an on-demand fashion	628:647	an on-demand fashion	628:647	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	6	41	theme	conventional	1173:1184	arg1	systems					1186:1192	the conventional systems	1169:1192	the conventional systems based on the petroleum-based polymers	1169:1230	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	4	42	theme	pH	680:681	arg1	conditions					683:692	lower pH conditions	674:692	lower pH conditions	674:692	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	1	43	theme	drug	216:219	arg1	delivery					221:228	on-demand drug delivery	206:228	on-demand drug delivery	206:228	In this study, we reported a pH/near-infrared (NIR)-responsive hydrogel for on-demand drug delivery and wound healing.					
29525166	2	44	theme	Cellulose	249:257	arg1	TOCNFs					272:277	TOCNFs	272:277	TOCNFs	272:277	Cellulose nanofibrils (TOCNFs) were produced by 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation method.					
29525166	2	44	theme	Cellulose	249:257	arg1	nanofibrils					259:269	Cellulose nanofibrils	249:269	Cellulose nanofibrils (TOCNFs)	249:278	Cellulose nanofibrils (TOCNFs) were produced by 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation method.					
29525166	4	45	theme	NIR	655:657	arg1	exposure					659:666	NIR exposure	655:666	NIR exposure	655:666	Then, tetracycline hydrochloride (TH) which loaded on PDA could be released from the prepared hydrogels in an on-demand fashion under NIR exposure or at lower pH conditions.					
29525166	1	46	theme	wound	234:238	arg1	healing					240:246	wound healing	234:246	wound healing	234:246	In this study, we reported a pH/near-infrared (NIR)-responsive hydrogel for on-demand drug delivery and wound healing.					
29525166	5	47	theme	in	712:713	arg1	experiments					732:742	the in vivo skin defect experiments	708:742	the in vivo skin defect experiments	708:742	In addition, the in vivo skin defect experiments illustrated that the resultant composite hydrogel had a synergistic effect on promoting wound healing.					
29525166	6	48	theme	pH/NIR	878:883	arg1	drug					918:921	long time period drug	901:921	long time period drug releasing property, antibacterial and mechanical properties	901:981	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
29525166	6	48	theme	pH/NIR	878:883	arg1	responsiveness					885:898	the unique pH/NIR responsiveness	867:898	the unique pH/NIR responsiveness	867:898	Therefore, owing to the unique pH/NIR responsiveness, long time period drug releasing property, antibacterial and mechanical properties, together with their biodegradability and biocompatibility, the prepared PDA/TOCNFs hydrogel is highly promising as a new drug delivery vehicle, offering simple and safe alternatives to the conventional systems based on the petroleum-based polymers.					
31483512	11	0	theme	high	1394:1397	arg1	intensity					1399:1407	high intensity	1394:1407	high intensity	1394:1407	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	0	1	with	patients	79:86	arg1	fibrillation					100:111	atrial fibrillation	93:111	atrial fibrillation	93:111	The effect of heparin infusion intensity on outcomes for bridging hospitalized patients with atrial fibrillation.					
31483512	14	2	theme	UFH	1713:1715	arg1	infusions					1717:1725	CONCLUSIONS Low intensity UFH infusions	1687:1725	CONCLUSIONS Low intensity UFH infusions	1687:1725	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	4	3	theme	low	617:619	arg1	regimens					631:638	low intensity regimens	617:638	low intensity regimens for bridging patients with AF	617:668	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31483512	11	4	theme	UFH	1435:1437	arg1	regimens					1439:1446	low intensity UFH regimens	1421:1446	low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90)	1421:1518	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	2	5	theme	bridging	286:293	arg1	practice					274:281	the practice	270:281	the practice of bridging	270:293	Recent guidance cautions the practice of bridging except in high risk patients.					
31483512	14	6	theme	Low	1699:1701	arg1	infusions					1717:1725	CONCLUSIONS Low intensity UFH infusions	1687:1725	CONCLUSIONS Low intensity UFH infusions	1687:1725	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	11	7	theme	low	1421:1423	arg1	regimens					1439:1446	low intensity UFH regimens	1421:1446	low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90)	1421:1518	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	2	8	theme	risk	310:313	arg1	patients					315:322	high risk patients	305:322	high risk patients	305:322	Recent guidance cautions the practice of bridging except in high risk patients.					
31483512	14	9	theme	CONCLUSIONS	1687:1697	arg1	infusions					1717:1725	CONCLUSIONS Low intensity UFH infusions	1687:1725	CONCLUSIONS Low intensity UFH infusions	1687:1725	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	1	10	theme	thromboembolic	199:212	arg1	rates					214:218	low thromboembolic rates	195:218	low thromboembolic rates	195:218	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	14	11	theme	events	1860:1865	arg1	signal					1825:1830	a signal	1823:1830	a signal of increased thromboembolic events in hospitalized AF patients	1823:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	5	12	theme	patients	747:754	arg1	study					729:733	a single center retrospective cohort study	692:733	a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours	692:803	METHODS We conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours.					
31483512	14	13	theme	hospitalized	1870:1881	arg1	patients					1886:1893	hospitalized AF patients	1870:1893	hospitalized AF patients	1870:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	11	14	theme	bleed	1371:1375	arg1	rate					1359:1362	The rate	1355:1362	The rate of any bleed	1355:1375	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	11	14	theme	bleed	1371:1375	arg1	higher					1381:1386	higher	1381:1386	higher	1381:1386	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	5	15	theme	center	701:706	arg1	study					729:733	a single center retrospective cohort study	692:733	a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours	692:803	METHODS We conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours.					
31483512	1	16	theme	Perioperative	125:137	arg1	BACKGROUND					114:123	BACKGROUND Perioperative bridging	114:146	BACKGROUND Perioperative bridging in atrial fibrillation (AF)	114:174	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	5	17	theme	cohort	722:727	arg1	study					729:733	a single center retrospective cohort study	692:733	a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours	692:803	METHODS We conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours.					
31483512	7	18	theme	discontinuation	1018:1032	arg1	24 hours					1006:1013	24 hours	1006:1013	24 hours of discontinuation	1006:1032	The primary endpoint was the rate of bleeding defined by the International Society on Thrombosis and Hemostasis during UFH infusion or within 24 hours of discontinuation.					
31483512	14	19	theme	increased	1835:1843	arg1	events					1860:1865	increased thromboembolic events	1835:1865	increased thromboembolic events in hospitalized AF patients	1835:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	14	20	theme	thromboembolic	1845:1858	arg1	events					1860:1865	increased thromboembolic events	1835:1865	increased thromboembolic events in hospitalized AF patients	1835:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	14	21	from	signal	1825:1830	arg1	patients					1886:1893	hospitalized AF patients	1870:1893	hospitalized AF patients	1870:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	2	22	theme	Recent	245:250	arg1	cautions					261:268	Recent guidance cautions	245:268	Recent guidance cautions	245:268	Recent guidance cautions the practice of bridging except in high risk patients.					
31483512	11	23	theme	confidence	1487:1496	arg1	interval					1498:1505	95% confidence interval	1483:1505	95% confidence interval	1483:1505	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	0	24	theme	hospitalized	66:77	arg1	patients					79:86	bridging hospitalized patients	57:86	bridging hospitalized patients with atrial fibrillation	57:111	The effect of heparin infusion intensity on outcomes for bridging hospitalized patients with atrial fibrillation.					
31483512	3	25	theme	bridging	350:357	arg1	practice					338:345	the practice	334:345	the practice of bridging	334:357	However, the practice of bridging varies widely and little data exist regarding appropriate anticoagulation intensity when using intravenous unfractionated heparin (UFH).					
31483512	4	26	theme	high	523:526	arg1	regimens					551:558	high intensity UFH infusion regimens	523:558	high intensity UFH infusion regimens	523:558	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31483512	11	27	theme	95	1483:1484	arg1	%					1485:1485	%	1485:1485	%	1485:1485	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	5	28	theme	non-valvular	761:772	arg1	AF					774:775	non-valvular AF	761:775	non-valvular AF receiving UFH for ≥24 hours	761:803	METHODS We conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours.					
31483512	7	29	theme	UFH	983:985	arg1	infusion					987:994	UFH infusion	983:994	UFH infusion	983:994	The primary endpoint was the rate of bleeding defined by the International Society on Thrombosis and Hemostasis during UFH infusion or within 24 hours of discontinuation.					
31483512	8	30	theme	events	1092:1097	arg1	infarctions					1161:1171	myocardial infarctions	1150:1171	myocardial infarctions	1150:1171	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	30	theme	events	1092:1097	arg1	composite					1064:1072	a composite	1062:1072	a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death	1062:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	30	theme	events	1092:1097	arg1	death					1177:1181	death	1177:1181	death	1177:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	30	theme	events	1092:1097	arg1	thromboembolism					1133:1147	venous thromboembolism	1126:1147	venous thromboembolism	1126:1147	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	30	theme	events	1092:1097	arg1	endpoint					1049:1056	The secondary endpoint	1035:1056	The secondary endpoint	1035:1056	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	30	theme	events	1092:1097	arg1	events					1092:1097	cardiovascular events	1077:1097	cardiovascular events	1077:1097	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	30	theme	events	1092:1097	arg1	thromboembolism					1109:1123	arterial thromboembolism	1100:1123	arterial thromboembolism	1100:1123	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	3	31	theme	little	377:382	arg1	data					384:387	little data	377:387	little data	377:387	However, the practice of bridging varies widely and little data exist regarding appropriate anticoagulation intensity when using intravenous unfractionated heparin (UFH).					
31483512	13	32	theme	thrombotic	1659:1668	arg1	events					1670:1675	composite thrombotic events	1649:1675	composite thrombotic events	1649:1675	There was no difference in composite thrombotic events or death.					
31483512	10	33	theme	acting	1306:1311	arg1	anticoagulants					1318:1331	direct acting oral anticoagulants	1299:1331	direct acting oral anticoagulants	1299:1331	Warfarin was used in 82.1% and direct acting oral anticoagulants in 14.1% of patients.					
31483512	8	34	theme	arterial	1100:1107	arg1	thromboembolism					1109:1123	arterial thromboembolism	1100:1123	arterial thromboembolism	1100:1123	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	1	35	from	BACKGROUND	114:123	arg1	fibrillation					158:169	atrial fibrillation	151:169	atrial fibrillation (AF)	151:174	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	1	35	from	BACKGROUND	114:123	arg1	AF					172:173	AF	172:173	AF	172:173	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	12	36	theme	UFH	1608:1610	arg1	regimens					1612:1619	low intensity UFH regimens	1594:1619	low intensity UFH regimens	1594:1619	Major bleeding was significantly higher among high intensity compared to low intensity UFH regimens.					
31483512	5	37	dep	METHODS	671:677	arg1	conducted					682:690	conducted	682:690	conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours	682:803	METHODS We conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours.					
31483512	14	38	theme	decreased	1790:1798	arg1	rates					1809:1813	decreased bleeding rates	1790:1813	decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients	1790:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	9	39	dep	RESULTS	1204:1210	arg1	total					1214:1218	A total	1212:1218	RESULTS A total of 497 patients	1204:1234	RESULTS A total of 497 patients were included in this analysis.					
31483512	5	40	with	patients	747:754	arg1	AF					774:775	non-valvular AF	761:775	non-valvular AF receiving UFH for ≥24 hours	761:803	METHODS We conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours.					
31483512	3	41	theme	appropriate	405:415	arg1	intensity					433:441	appropriate anticoagulation intensity	405:441	appropriate anticoagulation intensity	405:441	However, the practice of bridging varies widely and little data exist regarding appropriate anticoagulation intensity when using intravenous unfractionated heparin (UFH).					
31483512	4	42	theme	increased	580:588	arg1	rates					599:603	increased bleeding rates	580:603	increased bleeding rates compared to low intensity regimens for bridging patients with AF	580:668	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31483512	3	43	theme	unfractionated	466:479	arg1	heparin					481:487	intravenous unfractionated heparin	454:487	intravenous unfractionated heparin (UFH)	454:493	However, the practice of bridging varies widely and little data exist regarding appropriate anticoagulation intensity when using intravenous unfractionated heparin (UFH).					
31483512	3	43	theme	unfractionated	466:479	arg1	UFH					490:492	UFH	490:492	UFH	490:492	However, the practice of bridging varies widely and little data exist regarding appropriate anticoagulation intensity when using intravenous unfractionated heparin (UFH).					
31483512	0	44	theme	infusion	22:29	arg1	intensity					31:39	heparin infusion intensity	14:39	heparin infusion intensity	14:39	The effect of heparin infusion intensity on outcomes for bridging hospitalized patients with atrial fibrillation.					
31483512	8	45	theme	thromboembolism	1133:1147	arg1	infarctions					1161:1171	myocardial infarctions	1150:1171	myocardial infarctions	1150:1171	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	45	theme	thromboembolism	1133:1147	arg1	composite					1064:1072	a composite	1062:1072	a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death	1062:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	45	theme	thromboembolism	1133:1147	arg1	death					1177:1181	death	1177:1181	death	1177:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	45	theme	thromboembolism	1133:1147	arg1	thromboembolism					1133:1147	venous thromboembolism	1126:1147	venous thromboembolism	1126:1147	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	45	theme	thromboembolism	1133:1147	arg1	endpoint					1049:1056	The secondary endpoint	1035:1056	The secondary endpoint	1035:1056	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	45	theme	thromboembolism	1133:1147	arg1	events					1092:1097	cardiovascular events	1077:1097	cardiovascular events	1077:1097	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	45	theme	thromboembolism	1133:1147	arg1	thromboembolism					1109:1123	arterial thromboembolism	1100:1123	arterial thromboembolism	1100:1123	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	4	46	theme	UFH	538:540	arg1	regimens					551:558	high intensity UFH infusion regimens	523:558	high intensity UFH infusion regimens	523:558	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31483512	8	47	theme	myocardial	1150:1159	arg1	infarctions					1161:1171	myocardial infarctions	1150:1171	myocardial infarctions	1150:1171	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	1	48	theme	high	224:227	arg1	rates					238:242	high bleeding rates	224:242	high bleeding rates	224:242	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	4	49	theme	intensity	621:629	arg1	regimens					631:638	low intensity regimens	617:638	low intensity regimens for bridging patients with AF	617:668	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31483512	4	50	with	patients	653:660	arg1	AF					667:668	AF	667:668	AF	667:668	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31483512	10	51	theme	direct	1299:1304	arg1	anticoagulants					1318:1331	direct acting oral anticoagulants	1299:1331	direct acting oral anticoagulants	1299:1331	Warfarin was used in 82.1% and direct acting oral anticoagulants in 14.1% of patients.					
31483512	14	52	from	events	1860:1865	arg1	patients					1886:1893	hospitalized AF patients	1870:1893	hospitalized AF patients	1870:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	11	53	theme	intensity	1425:1433	arg1	regimens					1439:1446	low intensity UFH regimens	1421:1446	low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90)	1421:1518	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	9	54	theme	patients	1227:1234	arg1	total					1214:1218	A total	1212:1218	RESULTS A total of 497 patients	1204:1234	RESULTS A total of 497 patients were included in this analysis.					
31483512	7	55	theme	bleeding	901:908	arg1	endpoint					876:883	The primary endpoint	864:883	The primary endpoint	864:883	The primary endpoint was the rate of bleeding defined by the International Society on Thrombosis and Hemostasis during UFH infusion or within 24 hours of discontinuation.					
31483512	7	55	theme	bleeding	901:908	arg1	rate					893:896	the rate	889:896	the rate of bleeding defined by the International Society on Thrombosis and Hemostasis during UFH infusion or within 24 hours of discontinuation	889:1032	The primary endpoint was the rate of bleeding defined by the International Society on Thrombosis and Hemostasis during UFH infusion or within 24 hours of discontinuation.					
31483512	14	56	theme	intensity	1703:1711	arg1	infusions					1717:1725	CONCLUSIONS Low intensity UFH infusions	1687:1725	CONCLUSIONS Low intensity UFH infusions	1687:1725	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	8	57	theme	secondary	1039:1047	arg1	infarctions					1161:1171	myocardial infarctions	1150:1171	myocardial infarctions	1150:1171	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	57	theme	secondary	1039:1047	arg1	composite					1064:1072	a composite	1062:1072	a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death	1062:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	57	theme	secondary	1039:1047	arg1	death					1177:1181	death	1177:1181	death	1177:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	57	theme	secondary	1039:1047	arg1	thromboembolism					1133:1147	venous thromboembolism	1126:1147	venous thromboembolism	1126:1147	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	57	theme	secondary	1039:1047	arg1	endpoint					1049:1056	The secondary endpoint	1035:1056	The secondary endpoint	1035:1056	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	57	theme	secondary	1039:1047	arg1	events					1092:1097	cardiovascular events	1077:1097	cardiovascular events	1077:1097	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	57	theme	secondary	1039:1047	arg1	thromboembolism					1109:1123	arterial thromboembolism	1100:1123	arterial thromboembolism	1100:1123	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	4	58	theme	intensity	528:536	arg1	regimens					551:558	high intensity UFH infusion regimens	523:558	high intensity UFH infusion regimens	523:558	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31483512	6	59	theme	UFH	806:808	arg1	intensities					810:820	UFH intensities	806:820	UFH intensities	806:820	UFH intensities were chosen at the providers' discretion.					
31483512	2	60	theme	high	305:308	arg1	patients					315:322	high risk patients	305:322	high risk patients	305:322	Recent guidance cautions the practice of bridging except in high risk patients.					
31483512	7	61	theme	primary	868:874	arg1	endpoint					876:883	The primary endpoint	864:883	The primary endpoint	864:883	The primary endpoint was the rate of bleeding defined by the International Society on Thrombosis and Hemostasis during UFH infusion or within 24 hours of discontinuation.					
31483512	7	61	theme	primary	868:874	arg1	rate					893:896	the rate	889:896	the rate of bleeding defined by the International Society on Thrombosis and Hemostasis during UFH infusion or within 24 hours of discontinuation	889:1032	The primary endpoint was the rate of bleeding defined by the International Society on Thrombosis and Hemostasis during UFH infusion or within 24 hours of discontinuation.					
31483512	1	62	theme	low	195:197	arg1	rates					214:218	low thromboembolic rates	195:218	low thromboembolic rates	195:218	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	12	63	theme	low	1594:1596	arg1	regimens					1612:1619	low intensity UFH regimens	1594:1619	low intensity UFH regimens	1594:1619	Major bleeding was significantly higher among high intensity compared to low intensity UFH regimens.					
31483512	5	64	theme	admitted	738:745	arg1	patients					747:754	admitted patients	738:754	admitted patients with non-valvular AF receiving UFH for ≥24 hours	738:803	METHODS We conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours.					
31483512	14	65	from	patients	1886:1893	arg1	signal					1825:1830	a signal	1823:1830	a signal of increased thromboembolic events in hospitalized AF patients	1823:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	14	66	theme	AF	1883:1884	arg1	patients					1886:1893	hospitalized AF patients	1870:1893	hospitalized AF patients	1870:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	11	67	dep	regimens	1439:1446	arg1	=					1507:1507	=	1507:1507	=	1507:1507	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	5	68	theme	single	694:699	arg1	study					729:733	a single center retrospective cohort study	692:733	a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours	692:803	METHODS We conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours.					
31483512	12	69	theme	high	1567:1570	arg1	intensity					1572:1580	high intensity	1567:1580	high intensity	1567:1580	Major bleeding was significantly higher among high intensity compared to low intensity UFH regimens.					
31483512	11	70	theme	=	1475:1475	arg1	ratio					1469:1473	odds ratio	1464:1473	odds ratio = 2.29	1464:1480	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	5	71	theme	retrospective	708:720	arg1	study					729:733	a single center retrospective cohort study	692:733	a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours	692:803	METHODS We conducted a single center retrospective cohort study of admitted patients with non-valvular AF receiving UFH for ≥24 hours.					
31483512	1	72	theme	bridging	139:146	arg1	BACKGROUND					114:123	BACKGROUND Perioperative bridging	114:146	BACKGROUND Perioperative bridging in atrial fibrillation (AF)	114:174	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	2	73	from	practice	274:281	arg1	patients					315:322	high risk patients	305:322	high risk patients	305:322	Recent guidance cautions the practice of bridging except in high risk patients.					
31483512	11	74	theme	odds	1464:1467	arg1	ratio					1469:1473	odds ratio	1464:1473	odds ratio = 2.29	1464:1480	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	0	75	theme	bridging	57:64	arg1	patients					79:86	bridging hospitalized patients	57:86	bridging hospitalized patients with atrial fibrillation	57:111	The effect of heparin infusion intensity on outcomes for bridging hospitalized patients with atrial fibrillation.					
31483512	2	76	theme	guidance	252:259	arg1	cautions					261:268	Recent guidance cautions	245:268	Recent guidance cautions	245:268	Recent guidance cautions the practice of bridging except in high risk patients.					
31483512	1	77	theme	atrial	151:156	arg1	fibrillation					158:169	atrial fibrillation	151:169	atrial fibrillation (AF)	151:174	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	1	77	theme	atrial	151:156	arg1	AF					172:173	AF	172:173	AF	172:173	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	10	78	used	used	1281:1284	arg2	Warfarin					1268:1275	Warfarin	1268:1275	Warfarin	1268:1275	Warfarin was used in 82.1% and direct acting oral anticoagulants in 14.1% of patients.					
31483512	14	79	theme	anticoagulation	1744:1758	arg1	targets					1760:1766	lower anticoagulation targets	1738:1766	lower anticoagulation targets	1738:1766	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	11	80	theme	%	1485:1485	arg1	interval					1498:1505	95% confidence interval	1483:1505	95% confidence interval	1483:1505	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	0	81	from	effect	4:9	arg1	outcomes					44:51	outcomes	44:51	outcomes for bridging hospitalized patients with atrial fibrillation	44:111	The effect of heparin infusion intensity on outcomes for bridging hospitalized patients with atrial fibrillation.					
31483512	0	82	theme	atrial	93:98	arg1	fibrillation					100:111	atrial fibrillation	93:111	atrial fibrillation	93:111	The effect of heparin infusion intensity on outcomes for bridging hospitalized patients with atrial fibrillation.					
31483512	8	83	theme	cardiovascular	1077:1090	arg1	events					1092:1097	cardiovascular events	1077:1097	cardiovascular events	1077:1097	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	11	84	dep	=	1475:1475	arg1	2.29					1477:1480	2.29	1477:1480	2.29	1477:1480	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	13	85	theme	composite	1649:1657	arg1	events					1670:1675	composite thrombotic events	1649:1675	composite thrombotic events	1649:1675	There was no difference in composite thrombotic events or death.					
31483512	14	86	theme	lower	1738:1742	arg1	targets					1760:1766	lower anticoagulation targets	1738:1766	lower anticoagulation targets	1738:1766	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	10	87	theme	oral	1313:1316	arg1	anticoagulants					1318:1331	direct acting oral anticoagulants	1299:1331	direct acting oral anticoagulants	1299:1331	Warfarin was used in 82.1% and direct acting oral anticoagulants in 14.1% of patients.					
31483512	3	88	theme	intravenous	454:464	arg1	heparin					481:487	intravenous unfractionated heparin	454:487	intravenous unfractionated heparin (UFH)	454:493	However, the practice of bridging varies widely and little data exist regarding appropriate anticoagulation intensity when using intravenous unfractionated heparin (UFH).					
31483512	3	88	theme	intravenous	454:464	arg1	UFH					490:492	UFH	490:492	UFH	490:492	However, the practice of bridging varies widely and little data exist regarding appropriate anticoagulation intensity when using intravenous unfractionated heparin (UFH).					
31483512	8	89	theme	thromboembolism	1109:1123	arg1	infarctions					1161:1171	myocardial infarctions	1150:1171	myocardial infarctions	1150:1171	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	89	theme	thromboembolism	1109:1123	arg1	composite					1064:1072	a composite	1062:1072	a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death	1062:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	89	theme	thromboembolism	1109:1123	arg1	death					1177:1181	death	1177:1181	death	1177:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	89	theme	thromboembolism	1109:1123	arg1	thromboembolism					1133:1147	venous thromboembolism	1126:1147	venous thromboembolism	1126:1147	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	89	theme	thromboembolism	1109:1123	arg1	endpoint					1049:1056	The secondary endpoint	1035:1056	The secondary endpoint	1035:1056	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	89	theme	thromboembolism	1109:1123	arg1	events					1092:1097	cardiovascular events	1077:1097	cardiovascular events	1077:1097	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	89	theme	thromboembolism	1109:1123	arg1	thromboembolism					1109:1123	arterial thromboembolism	1100:1123	arterial thromboembolism	1100:1123	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	10	90	theme	patients	1345:1352	arg1	%					1340:1340	14.1%	1336:1340	14.1% of patients	1336:1352	Warfarin was used in 82.1% and direct acting oral anticoagulants in 14.1% of patients.					
31483512	10	90	theme	patients	1345:1352	arg1	patients					1345:1352	patients	1345:1352	patients	1345:1352	Warfarin was used in 82.1% and direct acting oral anticoagulants in 14.1% of patients.					
31483512	0	91	theme	heparin	14:20	arg1	intensity					31:39	heparin infusion intensity	14:39	heparin infusion intensity	14:39	The effect of heparin infusion intensity on outcomes for bridging hospitalized patients with atrial fibrillation.					
31483512	14	92	theme	bleeding	1800:1807	arg1	rates					1809:1813	decreased bleeding rates	1790:1813	decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients	1790:1893	CONCLUSIONS Low intensity UFH infusions, targeting lower anticoagulation targets, were associated with decreased bleeding rates without a signal of increased thromboembolic events in hospitalized AF patients.					
31483512	8	93	theme	venous	1126:1131	arg1	thromboembolism					1133:1147	venous thromboembolism	1126:1147	venous thromboembolism	1126:1147	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	12	94	theme	intensity	1598:1606	arg1	regimens					1612:1619	low intensity UFH regimens	1594:1619	low intensity UFH regimens	1594:1619	Major bleeding was significantly higher among high intensity compared to low intensity UFH regimens.					
31483512	4	95	theme	bleeding	590:597	arg1	rates					599:603	increased bleeding rates	580:603	increased bleeding rates compared to low intensity regimens for bridging patients with AF	580:668	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31483512	0	96	theme	intensity	31:39	arg1	effect					4:9	The effect	0:9	The effect of heparin infusion intensity on outcomes for bridging hospitalized patients with atrial fibrillation	0:111	The effect of heparin infusion intensity on outcomes for bridging hospitalized patients with atrial fibrillation.					
31483512	3	97	theme	anticoagulation	417:431	arg1	intensity					433:441	appropriate anticoagulation intensity	405:441	appropriate anticoagulation intensity	405:441	However, the practice of bridging varies widely and little data exist regarding appropriate anticoagulation intensity when using intravenous unfractionated heparin (UFH).					
31483512	4	98	theme	infusion	542:549	arg1	regimens					551:558	high intensity UFH infusion regimens	523:558	high intensity UFH infusion regimens	523:558	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31483512	8	99	theme	infarctions	1161:1171	arg1	infarctions					1161:1171	myocardial infarctions	1150:1171	myocardial infarctions	1150:1171	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	99	theme	infarctions	1161:1171	arg1	composite					1064:1072	a composite	1062:1072	a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death	1062:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	99	theme	infarctions	1161:1171	arg1	death					1177:1181	death	1177:1181	death	1177:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	99	theme	infarctions	1161:1171	arg1	thromboembolism					1133:1147	venous thromboembolism	1126:1147	venous thromboembolism	1126:1147	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	99	theme	infarctions	1161:1171	arg1	endpoint					1049:1056	The secondary endpoint	1035:1056	The secondary endpoint	1035:1056	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	99	theme	infarctions	1161:1171	arg1	events					1092:1097	cardiovascular events	1077:1097	cardiovascular events	1077:1097	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	99	theme	infarctions	1161:1171	arg1	thromboembolism					1109:1123	arterial thromboembolism	1100:1123	arterial thromboembolism	1100:1123	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	100	theme	death	1177:1181	arg1	infarctions					1161:1171	myocardial infarctions	1150:1171	myocardial infarctions	1150:1171	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	100	theme	death	1177:1181	arg1	composite					1064:1072	a composite	1062:1072	a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death	1062:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	100	theme	death	1177:1181	arg1	death					1177:1181	death	1177:1181	death	1177:1181	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	100	theme	death	1177:1181	arg1	thromboembolism					1133:1147	venous thromboembolism	1126:1147	venous thromboembolism	1126:1147	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	100	theme	death	1177:1181	arg1	endpoint					1049:1056	The secondary endpoint	1035:1056	The secondary endpoint	1035:1056	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	100	theme	death	1177:1181	arg1	events					1092:1097	cardiovascular events	1077:1097	cardiovascular events	1077:1097	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	8	100	theme	death	1177:1181	arg1	thromboembolism					1109:1123	arterial thromboembolism	1100:1123	arterial thromboembolism	1100:1123	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	11	101	dep	=	1507:1507	arg1	%					1453:1453	10.5%	1449:1453	10.5%	1449:1453	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	11	101	dep	=	1507:1507	arg1	%					1461:1461	4.9%	1458:1461	4.9%	1458:1461	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	11	101	dep	=	1507:1507	arg1	ratio					1469:1473	odds ratio	1464:1473	odds ratio = 2.29	1464:1480	The rate of any bleed was higher among high intensity compared to low intensity UFH regimens (10.5% vs 4.9%, odds ratio = 2.29, 95% confidence interval = 1.07-4.90).					
31483512	1	102	theme	bleeding	229:236	arg1	rates					238:242	high bleeding rates	224:242	high bleeding rates	224:242	BACKGROUND Perioperative bridging in atrial fibrillation (AF) is associated with low thromboembolic rates but high bleeding rates.					
31483512	8	103	theme	UFH	1190:1192	arg1	infusion					1194:1201	UFH infusion	1190:1201	UFH infusion	1190:1201	The secondary endpoint was a composite of cardiovascular events, arterial thromboembolism, venous thromboembolism, myocardial infarctions and death during UFH infusion.					
31483512	4	104	theme	bridging	644:651	arg1	patients					653:660	bridging patients	644:660	bridging patients with AF	644:668	HYPOTHESIS To determine if high intensity UFH infusion regimens are associated with increased bleeding rates compared to low intensity regimens for bridging patients with AF.					
31757105	5	0	theme	same	715:718	arg1	sugars					720:725	The same sugars	711:725	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose	711:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	1	theme	cultivated	821:830	arg1	lingonberries					832:844	wild and cultivated lingonberries	812:844	wild and cultivated lingonberries	812:844	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	6	2	theme	tree	926:929	arg1	forests					931:937	dry pine tree forests	917:937	dry pine tree forests with lower altitude and latitude of the location	917:986	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	6	2	theme	tree	926:929	arg1	sun					912:914	full sun	907:914	full sun	907:914	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	0	3	theme	vitis-idaea	70:80	arg1	L					82:82	Vaccinium vitis-idaea L.	60:83	Vaccinium vitis-idaea L.	60:83	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	0	3	theme	vitis-idaea	70:80	arg1	Lingonberries					45:57	Wild and Cultivated Lingonberries	25:57	Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.)	25:84	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	6	4	theme	Cultivar	847:854	arg1	lingonberries					878:890	Cultivar 'Erntekrone' and wild lingonberries	847:890	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location,	847:987	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	1	5	theme	human	136:140	arg1	diet					142:145	the human diet	132:145	the human diet	132:145	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	3	6	from	cultivars	490:498	arg1	composition					449:459	the composition	445:459	the composition of sugars in wild clones and cultivars or lower taxa of lingonberries	445:529	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	1	7	theme	cosmetic	220:227	arg1	industries					248:257	the cosmetic and pharmaceutical industries	216:257	the cosmetic and pharmaceutical industries	216:257	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	8	8	theme	dietary	1286:1292	arg1	sugars					1294:1299	dietary sugars	1286:1299	dietary sugars	1286:1299	According to our findings, lingonberries seem to be an appropriate source of dietary sugars.					
31757105	0	9	from	Composition	0:10	arg1	L					82:82	Vaccinium vitis-idaea L.	60:83	Vaccinium vitis-idaea L.	60:83	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	0	9	from	Composition	0:10	arg1	Lingonberries					45:57	Wild and Cultivated Lingonberries	25:57	Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.)	25:84	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	3	10	from	sugars	464:469	arg1	cultivars					490:498	cultivars	490:498	cultivars	490:498	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	3	10	from	sugars	464:469	arg1	clones					479:484	wild clones	474:484	wild clones	474:484	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	3	11	theme	taxa	509:512	arg1	composition					449:459	the composition	445:459	the composition of sugars in wild clones and cultivars or lower taxa of lingonberries	445:529	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	6	12	theme	dry	917:919	arg1	forests					931:937	dry pine tree forests	917:937	dry pine tree forests with lower altitude and latitude of the location	917:986	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	6	12	theme	dry	917:919	arg1	sun					912:914	full sun	907:914	full sun	907:914	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	6	13	theme	full	907:910	arg1	forests					931:937	dry pine tree forests	917:937	dry pine tree forests with lower altitude and latitude of the location	917:986	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	6	13	theme	full	907:910	arg1	sun					912:914	full sun	907:914	full sun	907:914	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	6	14	theme	exclusive	1019:1027	arg1	contents					1034:1041	exclusive high contents	1019:1041	exclusive high contents of sugars	1019:1051	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	1	15	theme	pharmaceutical	233:246	arg1	industries					248:257	the cosmetic and pharmaceutical industries	216:257	the cosmetic and pharmaceutical industries	216:257	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	5	16	theme	low	775:777	arg1	amounts					779:785	low amounts	775:785	low amounts of sucrose	775:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	16	theme	low	775:777	arg1	sucrose					790:796	sucrose	790:796	sucrose	790:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	2	17	theme	high	382:385	arg1	consumption					393:403	high sugar consumption	382:403	high sugar consumption	382:403	It is important to examine the sugar profile of these berries, due to potential deleterious health effects resulting from high sugar consumption.					
31757105	8	18	theme	appropriate	1264:1274	arg1	lingonberries					1236:1248	lingonberries	1236:1248	lingonberries	1236:1248	According to our findings, lingonberries seem to be an appropriate source of dietary sugars.					
31757105	8	18	theme	appropriate	1264:1274	arg1	source					1276:1281	an appropriate source	1261:1281	an appropriate source of dietary sugars	1261:1299	According to our findings, lingonberries seem to be an appropriate source of dietary sugars.					
31757105	5	19	theme	sucrose	790:796	arg1	amounts					779:785	low amounts	775:785	low amounts of sucrose	775:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	19	theme	sucrose	790:796	arg1	glucose					762:768	glucose	762:768	glucose	762:768	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	19	theme	sucrose	790:796	arg1	sucrose					790:796	sucrose	790:796	sucrose	790:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	19	theme	sucrose	790:796	arg1	fructose					752:759	fructose	752:759	fructose	752:759	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	6	20	dep	Cultivar	847:854	arg1	Erntekrone					857:866	Erntekrone	857:866	Erntekrone	857:866	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	1	21	used	used	124:127	arg2	Products					87:94	Products	87:94	Products of lingonberries	87:111	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	2	22	theme	health	352:357	arg1	effects					359:365	potential deleterious health effects	330:365	potential deleterious health effects resulting from high sugar consumption	330:403	It is important to examine the sugar profile of these berries, due to potential deleterious health effects resulting from high sugar consumption.					
31757105	5	23	theme	fructose	752:759	arg1	amounts					741:747	moderate amounts	732:747	moderate amounts of fructose, glucose, and low amounts of sucrose	732:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	23	theme	fructose	752:759	arg1	amounts					779:785	low amounts	775:785	low amounts of sucrose	775:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	23	theme	fructose	752:759	arg1	glucose					762:768	glucose	762:768	glucose	762:768	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	23	theme	fructose	752:759	arg1	sucrose					790:796	sucrose	790:796	sucrose	790:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	23	theme	fructose	752:759	arg1	fructose					752:759	fructose	752:759	fructose	752:759	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	6	24	dep	lower	944:948	arg1	altitude					950:957	altitude	950:957	altitude	950:957	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	4	25	theme	method	689:694	arg1	precision					646:654	precision	646:654	precision	646:654	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	25	theme	method	689:694	arg1	linearity					595:603	linearity	595:603	linearity	595:603	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	25	theme	method	689:694	arg1	suitability					582:592	Acceptable system suitability	564:592	Acceptable system suitability	564:592	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	25	theme	method	689:694	arg1	limits					606:611	limits	606:611	limits of detection and quantification	606:643	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	25	theme	method	689:694	arg1	accuracy					661:668	accuracy	661:668	accuracy	661:668	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	2	26	theme	deleterious	340:350	arg1	effects					359:365	potential deleterious health effects	330:365	potential deleterious health effects resulting from high sugar consumption	330:403	It is important to examine the sugar profile of these berries, due to potential deleterious health effects resulting from high sugar consumption.					
31757105	7	27	theme	growing	1097:1103	arg1	season					1105:1110	the growing season	1093:1110	the growing season	1093:1110	The changes in the sugar levels during the growing season were apparent in lingonberries and the highest amounts accumulated at the end of the vegetation.					
31757105	7	28	theme	sugar	1073:1077	arg1	levels					1079:1084	the sugar levels	1069:1084	the sugar levels	1069:1084	The changes in the sugar levels during the growing season were apparent in lingonberries and the highest amounts accumulated at the end of the vegetation.					
31757105	3	29	theme	lingonberries	517:529	arg1	composition					449:459	the composition	445:459	the composition of sugars in wild clones and cultivars or lower taxa of lingonberries	445:529	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	2	30	theme	potential	330:338	arg1	effects					359:365	potential deleterious health effects	330:365	potential deleterious health effects resulting from high sugar consumption	330:403	It is important to examine the sugar profile of these berries, due to potential deleterious health effects resulting from high sugar consumption.					
31757105	5	31	theme	glucose	762:768	arg1	amounts					741:747	moderate amounts	732:747	moderate amounts of fructose, glucose, and low amounts of sucrose	732:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	31	theme	glucose	762:768	arg1	amounts					779:785	low amounts	775:785	low amounts of sucrose	775:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	31	theme	glucose	762:768	arg1	glucose					762:768	glucose	762:768	glucose	762:768	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	31	theme	glucose	762:768	arg1	sucrose					790:796	sucrose	790:796	sucrose	790:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	31	theme	glucose	762:768	arg1	fructose					752:759	fructose	752:759	fructose	752:759	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	4	32	theme	analytical	678:687	arg1	method					689:694	this analytical method	673:694	this analytical method	673:694	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	3	33	from	composition	449:459	arg1	cultivars					490:498	cultivars	490:498	cultivars	490:498	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	3	33	from	composition	449:459	arg1	clones					479:484	wild clones	474:484	wild clones	474:484	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	3	34	theme	lower	503:507	arg1	taxa					509:512	lower taxa	503:512	lower taxa	503:512	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	2	35	theme	sugar	387:391	arg1	consumption					393:403	high sugar consumption	382:403	high sugar consumption	382:403	It is important to examine the sugar profile of these berries, due to potential deleterious health effects resulting from high sugar consumption.					
31757105	0	36	theme	Sugars	15:20	arg1	Composition					0:10	Composition	0:10	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).	0:85	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	2	37	theme	berries	314:320	arg1	profile					297:303	the sugar profile	287:303	the sugar profile of these berries	287:320	It is important to examine the sugar profile of these berries, due to potential deleterious health effects resulting from high sugar consumption.					
31757105	1	38	theme	promising	162:170	arg1	candidates					202:211	promising beauty and health therapeutic candidates	162:211	promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries	162:257	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	1	38	theme	promising	162:170	arg1	they					148:151	they	148:151	they	148:151	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	7	39	theme	vegetation	1197:1206	arg1	end					1186:1188	the end	1182:1188	the end of the vegetation	1182:1206	The changes in the sugar levels during the growing season were apparent in lingonberries and the highest amounts accumulated at the end of the vegetation.					
31757105	5	40	theme	amounts	779:785	arg1	amounts					741:747	moderate amounts	732:747	moderate amounts of fructose, glucose, and low amounts of sucrose	732:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	40	theme	amounts	779:785	arg1	amounts					779:785	low amounts	775:785	low amounts of sucrose	775:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	40	theme	amounts	779:785	arg1	glucose					762:768	glucose	762:768	glucose	762:768	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	40	theme	amounts	779:785	arg1	sucrose					790:796	sucrose	790:796	sucrose	790:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	40	theme	amounts	779:785	arg1	fructose					752:759	fructose	752:759	fructose	752:759	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	4	41	theme	detection	616:624	arg1	precision					646:654	precision	646:654	precision	646:654	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	41	theme	detection	616:624	arg1	linearity					595:603	linearity	595:603	linearity	595:603	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	41	theme	detection	616:624	arg1	suitability					582:592	Acceptable system suitability	564:592	Acceptable system suitability	564:592	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	41	theme	detection	616:624	arg1	limits					606:611	limits	606:611	limits of detection and quantification	606:643	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	41	theme	detection	616:624	arg1	accuracy					661:668	accuracy	661:668	accuracy	661:668	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	1	42	theme	beauty	172:177	arg1	candidates					202:211	promising beauty and health therapeutic candidates	162:211	promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries	162:257	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	1	42	theme	beauty	172:177	arg1	they					148:151	they	148:151	they	148:151	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	0	43	theme	Wild	25:28	arg1	L					82:82	Vaccinium vitis-idaea L.	60:83	Vaccinium vitis-idaea L.	60:83	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	0	43	theme	Wild	25:28	arg1	Lingonberries					45:57	Wild and Cultivated Lingonberries	25:57	Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.)	25:84	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	5	44	theme	moderate	732:739	arg1	amounts					741:747	moderate amounts	732:747	moderate amounts of fructose, glucose, and low amounts of sucrose	732:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	44	theme	moderate	732:739	arg1	amounts					779:785	low amounts	775:785	low amounts of sucrose	775:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	44	theme	moderate	732:739	arg1	glucose					762:768	glucose	762:768	glucose	762:768	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	44	theme	moderate	732:739	arg1	sucrose					790:796	sucrose	790:796	sucrose	790:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	44	theme	moderate	732:739	arg1	fructose					752:759	fructose	752:759	fructose	752:759	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	6	45	theme	pine	921:924	arg1	forests					931:937	dry pine tree forests	917:937	dry pine tree forests with lower altitude and latitude of the location	917:986	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	6	45	theme	pine	921:924	arg1	sun					912:914	full sun	907:914	full sun	907:914	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	6	46	with	forests	931:937	arg1	lower					944:948	lower	944:948	lower	944:948	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	6	46	with	forests	931:937	arg1	latitude					963:970	latitude	963:970	latitude	963:970	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	6	47	theme	wild	873:876	arg1	lingonberries					878:890	Cultivar 'Erntekrone' and wild lingonberries	847:890	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location,	847:987	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	1	48	from	candidates	202:211	arg1	industries					248:257	the cosmetic and pharmaceutical industries	216:257	the cosmetic and pharmaceutical industries	216:257	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	5	49	located	found	803:807	arg2	sugars					720:725	The same sugars	711:725	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose	711:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	49	located	found	803:807	arg1	lingonberries					832:844	wild and cultivated lingonberries	812:844	wild and cultivated lingonberries	812:844	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	0	50	theme	Cultivated	34:43	arg1	L					82:82	Vaccinium vitis-idaea L.	60:83	Vaccinium vitis-idaea L.	60:83	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	0	50	theme	Cultivated	34:43	arg1	Lingonberries					45:57	Wild and Cultivated Lingonberries	25:57	Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.)	25:84	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	7	51	from	lingonberries	1129:1141	arg1	apparent					1117:1124	apparent	1117:1124	apparent	1117:1124	The changes in the sugar levels during the growing season were apparent in lingonberries and the highest amounts accumulated at the end of the vegetation.					
31757105	7	51	from	lingonberries	1129:1141	arg1	changes					1058:1064	The changes	1054:1064	The changes in the sugar levels during the growing season	1054:1110	The changes in the sugar levels during the growing season were apparent in lingonberries and the highest amounts accumulated at the end of the vegetation.					
31757105	3	52	theme	HPLC-ELSD	534:542	arg1	method					544:549	HPLC-ELSD method	534:549	HPLC-ELSD method of analysis	534:561	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	3	53	theme	sugars	464:469	arg1	composition					449:459	the composition	445:459	the composition of sugars in wild clones and cultivars or lower taxa of lingonberries	445:529	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	1	54	theme	lingonberries	99:111	arg1	Products					87:94	Products	87:94	Products of lingonberries	87:111	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	4	55	theme	quantification	630:643	arg1	precision					646:654	precision	646:654	precision	646:654	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	55	theme	quantification	630:643	arg1	linearity					595:603	linearity	595:603	linearity	595:603	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	55	theme	quantification	630:643	arg1	suitability					582:592	Acceptable system suitability	564:592	Acceptable system suitability	564:592	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	55	theme	quantification	630:643	arg1	limits					606:611	limits	606:611	limits of detection and quantification	606:643	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	4	55	theme	quantification	630:643	arg1	accuracy					661:668	accuracy	661:668	accuracy	661:668	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	6	56	theme	sugars	1046:1051	arg1	contents					1034:1041	exclusive high contents	1019:1041	exclusive high contents of sugars	1019:1051	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	3	57	from	taxa	509:512	arg1	cultivars					490:498	cultivars	490:498	cultivars	490:498	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	3	57	from	taxa	509:512	arg1	clones					479:484	wild clones	474:484	wild clones	474:484	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	1	58	theme	health	183:188	arg1	candidates					202:211	promising beauty and health therapeutic candidates	162:211	promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries	162:257	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	1	58	theme	health	183:188	arg1	they					148:151	they	148:151	they	148:151	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	7	59	from	apparent	1117:1124	arg1	lingonberries					1129:1141	lingonberries	1129:1141	lingonberries	1129:1141	The changes in the sugar levels during the growing season were apparent in lingonberries and the highest amounts accumulated at the end of the vegetation.					
31757105	5	60	with	sugars	720:725	arg1	amounts					741:747	moderate amounts	732:747	moderate amounts of fructose, glucose, and low amounts of sucrose	732:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	60	with	sugars	720:725	arg1	amounts					779:785	low amounts	775:785	low amounts of sucrose	775:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	60	with	sugars	720:725	arg1	glucose					762:768	glucose	762:768	glucose	762:768	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	60	with	sugars	720:725	arg1	sucrose					790:796	sucrose	790:796	sucrose	790:796	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	5	60	with	sugars	720:725	arg1	fructose					752:759	fructose	752:759	fructose	752:759	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	2	61	theme	sugar	291:295	arg1	profile					297:303	the sugar profile	287:303	the sugar profile of these berries	287:320	It is important to examine the sugar profile of these berries, due to potential deleterious health effects resulting from high sugar consumption.					
31757105	1	62	theme	therapeutic	190:200	arg1	candidates					202:211	promising beauty and health therapeutic candidates	162:211	promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries	162:257	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	1	62	theme	therapeutic	190:200	arg1	they					148:151	they	148:151	they	148:151	Products of lingonberries are widely used in the human diet; they are also promising beauty and health therapeutic candidates in the cosmetic and pharmaceutical industries.					
31757105	0	63	theme	Vaccinium	60:68	arg1	L					82:82	Vaccinium vitis-idaea L.	60:83	Vaccinium vitis-idaea L.	60:83	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	0	63	theme	Vaccinium	60:68	arg1	Lingonberries					45:57	Wild and Cultivated Lingonberries	25:57	Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.)	25:84	Composition of Sugars in Wild and Cultivated Lingonberries (Vaccinium vitis-idaea L.).					
31757105	6	64	theme	high	1029:1032	arg1	contents					1034:1041	exclusive high contents	1019:1041	exclusive high contents of sugars	1019:1051	Cultivar 'Erntekrone' and wild lingonberries collected from full sun, dry pine tree forests with lower altitude and latitude of the location, distinguished themselves with exclusive high contents of sugars.					
31757105	3	65	theme	analysis	554:561	arg1	method					544:549	HPLC-ELSD method	534:549	HPLC-ELSD method of analysis	534:561	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	3	66	theme	study	422:426	arg1	aim					410:412	The aim	406:412	The aim of this study	406:426	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	5	67	theme	wild	812:815	arg1	lingonberries					832:844	wild and cultivated lingonberries	812:844	wild and cultivated lingonberries	812:844	The same sugars with moderate amounts of fructose, glucose, and low amounts of sucrose were found in wild and cultivated lingonberries.					
31757105	8	68	theme	sugars	1294:1299	arg1	lingonberries					1236:1248	lingonberries	1236:1248	lingonberries	1236:1248	According to our findings, lingonberries seem to be an appropriate source of dietary sugars.					
31757105	8	68	theme	sugars	1294:1299	arg1	source					1276:1281	an appropriate source	1261:1281	an appropriate source of dietary sugars	1261:1299	According to our findings, lingonberries seem to be an appropriate source of dietary sugars.					
31757105	4	69	theme	system	575:580	arg1	suitability					582:592	Acceptable system suitability	564:592	Acceptable system suitability	564:592	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
31757105	7	70	theme	highest	1151:1157	arg1	amounts					1159:1165	the highest amounts	1147:1165	the highest amounts	1147:1165	The changes in the sugar levels during the growing season were apparent in lingonberries and the highest amounts accumulated at the end of the vegetation.					
31757105	7	71	from	changes	1058:1064	arg1	levels					1079:1084	the sugar levels	1069:1084	the sugar levels	1069:1084	The changes in the sugar levels during the growing season were apparent in lingonberries and the highest amounts accumulated at the end of the vegetation.					
31757105	3	72	theme	wild	474:477	arg1	clones					479:484	wild clones	474:484	wild clones	474:484	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	3	73	from	clones	479:484	arg1	composition					449:459	the composition	445:459	the composition of sugars in wild clones and cultivars or lower taxa of lingonberries	445:529	The aim of this study was to determine the composition of sugars in wild clones and cultivars or lower taxa of lingonberries by HPLC-ELSD method of analysis.					
31757105	4	74	theme	Acceptable	564:573	arg1	suitability					582:592	Acceptable system suitability	564:592	Acceptable system suitability	564:592	Acceptable system suitability, linearity, limits of detection and quantification, precision, and accuracy of this analytical method were achieved.					
29622215	1	0	theme	antinutrients	144:156	arg1	inclusion					131:139	The unplanned inclusion	117:139	The unplanned inclusion of antinutrients in fish food	117:169	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	1	1	theme	acids	237:241	arg1	digestibility					214:226	digestibility	214:226	digestibility of amino acids	214:241	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	1	1	theme	acids	237:241	arg1	conversion					252:261	diet conversion	247:261	diet conversion	247:261	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	0	2	theme	new	70:72	arg1	tool					74:77	a new tool	68:77	a new tool to detect antinutrients in aquafeeds	68:114	Use of fish trypsin immobilized onto magnetic-chitosan composite as a new tool to detect antinutrients in aquafeeds.					
29622215	1	3	from	effects	289:295	arg1	growth					305:310	body growth	300:310	body growth	300:310	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	1	4	theme	diet	247:250	arg1	conversion					252:261	diet conversion	247:261	diet conversion	247:261	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	4	5	theme	magnetic	879:886	arg1	chitosan					888:895	magnetic chitosan	879:895	magnetic chitosan	879:895	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	4	6	from	diets	814:818	arg1	present					785:791	present	785:791	present	785:791	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	1	7	from	inclusion	131:139	arg1	food					166:169	fish food	161:169	fish food	161:169	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	2	8	theme	antinutrients	470:482	arg1	antinutrients					470:482	the most important antinutrients	451:482	the most important antinutrients	451:482	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	2	8	theme	antinutrients	470:482	arg1	one					444:446	one	444:446	one	444:446	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	2	8	theme	antinutrients	470:482	arg1	detection					410:418	the detection	406:418	the detection of protease inhibitors	406:441	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	1	9	theme	fish	161:164	arg1	food					166:169	fish food	161:169	fish food	161:169	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	2	10	theme	important	460:468	arg1	antinutrients					470:482	the most important antinutrients	451:482	the most important antinutrients	451:482	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	5	11	theme	antinutritional	1038:1052	arg1	factors					1054:1060	antinutritional factors	1038:1060	antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry	1038:1155	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	5	12	theme	factors	1054:1060	arg1	detection					1025:1033	the detection	1021:1033	the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry	1021:1155	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	3	13	theme	antinutritional	523:537	arg1	factors					539:545	antinutritional factors	523:545	antinutritional factors	523:545	In order to evaluate the detection of antinutritional factors through the immobilized trypsin, the enzyme was incubated with eight diets developed for commercial fish, and residual activity was measured.					
29622215	4	14	theme	studied	806:812	arg1	diets					814:818	the eight studied diets	796:818	the eight studied diets	796:818	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	3	15	theme	factors	539:545	arg1	detection					510:518	the detection	506:518	the detection of antinutritional factors through the immobilized trypsin	506:577	In order to evaluate the detection of antinutritional factors through the immobilized trypsin, the enzyme was incubated with eight diets developed for commercial fish, and residual activity was measured.					
29622215	1	16	theme	many	179:182	arg1	processes					195:203	many biological processes	179:203	many biological processes	179:203	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	1	16	theme	many	179:182	arg1	digestibility					214:226	digestibility	214:226	digestibility of amino acids	214:241	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	1	16	theme	many	179:182	arg1	conversion					252:261	diet conversion	247:261	diet conversion	247:261	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	0	17	theme	trypsin	12:18	arg1	Use					0:2	Use	0:2	Use of fish trypsin	0:18	Use of fish trypsin immobilized onto magnetic-chitosan composite as a new tool to detect antinutrients in aquafeeds.					
29622215	5	18	theme	specific	976:983	arg1	proteases					985:993	specific proteases	976:993	specific proteases of the target organism	976:1016	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	2	19	theme	fish	388:391	arg1	proteases					393:401	immobilized fish proteases	376:401	immobilized fish proteases	376:401	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	2	20	theme	proteases	393:401	arg1	use					369:371	the use	365:371	the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients	365:482	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	4	21	theme	porcine	848:854	arg1	trypsin					856:862	the porcine trypsin	844:862	the porcine trypsin immobilized in magnetic chitosan	844:895	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	5	22	theme	target	1002:1007	arg1	organism					1009:1016	the target organism	998:1016	the target organism	998:1016	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	4	23	theme	tilapia	708:714	arg1	trypsin					716:722	the tilapia trypsin	704:722	the tilapia trypsin	704:722	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	2	24	theme	immobilized	376:386	arg1	proteases					393:401	immobilized fish proteases	376:401	immobilized fish proteases	376:401	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	4	25	dep	48	827:828	arg1	to					824:825	to	824:825	to	824:825	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	1	26	theme	undesirable	277:287	arg1	effects					289:295	undesirable effects	277:295	undesirable effects on body growth	277:310	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	5	27	theme	organism	1009:1016	arg1	proteases					985:993	specific proteases	976:993	specific proteases of the target organism	976:1016	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	3	28	theme	immobilized	559:569	arg1	trypsin					571:577	the immobilized trypsin	555:577	the immobilized trypsin	555:577	In order to evaluate the detection of antinutritional factors through the immobilized trypsin, the enzyme was incubated with eight diets developed for commercial fish, and residual activity was measured.					
29622215	2	29	from	use	369:371	arg1	antinutrients					470:482	the most important antinutrients	451:482	the most important antinutrients	451:482	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	2	29	from	use	369:371	arg1	one					444:446	one	444:446	one	444:446	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	2	29	from	use	369:371	arg1	detection					410:418	the detection	406:418	the detection of protease inhibitors	406:441	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	2	30	theme	inhibitors	432:441	arg1	antinutrients					470:482	the most important antinutrients	451:482	the most important antinutrients	451:482	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	2	30	theme	inhibitors	432:441	arg1	one					444:446	one	444:446	one	444:446	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	2	30	theme	inhibitors	432:441	arg1	detection					410:418	the detection	406:418	the detection of protease inhibitors	406:441	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	5	31	theme	digestive	1083:1091	arg1	capacity					1093:1100	animal's digestive capacity	1074:1100	animal's digestive capacity	1074:1100	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	5	32	theme	immobilized	941:951	arg1	derivatives					953:963	immobilized derivatives	941:963	immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry	941:1155	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	2	33	theme	protease	423:430	arg1	inhibitors					432:441	protease inhibitors	423:441	protease inhibitors	423:441	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	4	34	theme	greater	831:837	arg1	inhibition					734:743	an inhibition	731:743	an inhibition	731:743	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	5	35	theme	derivatives	953:963	arg1	use					934:936	the use	930:936	the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry	930:1155	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	4	36	from	present	785:791	arg1	diets					814:818	the eight studied diets	796:818	the eight studied diets	796:818	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	0	37	theme	magnetic-chitosan	37:53	arg1	composite					55:63	magnetic-chitosan composite	37:63	magnetic-chitosan composite	37:63	Use of fish trypsin immobilized onto magnetic-chitosan composite as a new tool to detect antinutrients in aquafeeds.					
29622215	1	38	theme	body	300:303	arg1	growth					305:310	body growth	300:310	body growth	300:310	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	3	39	theme	commercial	636:645	arg1	fish					647:650	commercial fish	636:650	commercial fish	636:650	In order to evaluate the detection of antinutritional factors through the immobilized trypsin, the enzyme was incubated with eight diets developed for commercial fish, and residual activity was measured.					
29622215	4	40	theme	present	785:791	arg1	inhibition					734:743	an inhibition	731:743	an inhibition	731:743	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	5	41	contain	containing	965:974	arg1	derivatives					953:963	immobilized derivatives	941:963	immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry	941:1155	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	5	41	contain	containing	965:974	arg2	proteases					985:993	specific proteases	976:993	specific proteases of the target organism	976:1016	Thus, it is possible to suggest the use of immobilized derivatives containing specific proteases of the target organism in the detection of antinutritional factors that reduce animal's digestive capacity and negatively influence their growth during husbandry.					
29622215	4	42	theme	protease	763:770	arg1	antinutrients					748:760	antinutrients	748:760	antinutrients (protease inhibitors)	748:782	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	4	42	theme	protease	763:770	arg1	inhibitors					772:781	protease inhibitors	763:781	protease inhibitors	763:781	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	0	43	from	antinutrients	89:101	arg1	aquafeeds					106:114	aquafeeds	106:114	aquafeeds	106:114	Use of fish trypsin immobilized onto magnetic-chitosan composite as a new tool to detect antinutrients in aquafeeds.					
29622215	4	44	theme	antinutrients	748:760	arg1	inhibition					734:743	an inhibition	731:743	an inhibition	731:743	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	1	45	theme	unplanned	121:129	arg1	inclusion					131:139	The unplanned inclusion	117:139	The unplanned inclusion of antinutrients in fish food	117:169	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	1	46	theme	biological	184:193	arg1	processes					195:203	many biological processes	179:203	many biological processes	179:203	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	1	46	theme	biological	184:193	arg1	digestibility					214:226	digestibility	214:226	digestibility of amino acids	214:241	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	1	46	theme	biological	184:193	arg1	conversion					252:261	diet conversion	247:261	diet conversion	247:261	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
29622215	2	47	theme	research	341:348	arg1	objective					323:331	the objective	319:331	the objective of this research	319:348	Thus, the objective of this research was to propose the use of immobilized fish proteases in the detection of protease inhibitors, one of the most important antinutrients.					
29622215	4	48	attach	present	785:791	arg1	diets					814:818	the eight studied diets	796:818	the eight studied diets	796:818	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	4	48	attach	present	785:791	arg2	inhibition					734:743	an inhibition	731:743	an inhibition	731:743	Comparatively, the tilapia trypsin showed an inhibition of antinutrients (protease inhibitors), present in the eight studied diets, up to 48% greater than the porcine trypsin immobilized in magnetic chitosan.					
29622215	3	49	theme	residual	657:664	arg1	activity					666:673	residual activity	657:673	residual activity	657:673	In order to evaluate the detection of antinutritional factors through the immobilized trypsin, the enzyme was incubated with eight diets developed for commercial fish, and residual activity was measured.					
29622215	0	50	theme	fish	7:10	arg1	trypsin					12:18	fish trypsin	7:18	fish trypsin	7:18	Use of fish trypsin immobilized onto magnetic-chitosan composite as a new tool to detect antinutrients in aquafeeds.					
29622215	1	51	theme	amino	231:235	arg1	acids					237:241	amino acids	231:241	amino acids	231:241	The unplanned inclusion of antinutrients in fish food affects many biological processes, such as digestibility of amino acids and diet conversion, resulting in undesirable effects on body growth.					
30807801	6	0	theme	@	797:797	arg1	composite					803:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	1	1	theme	hydrochloric	200:211	arg1	H					233:233	Nano-CS/Clino@H	219:233	Nano-CS/Clino@H	219:233	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	1	theme	hydrochloric	200:211	arg1	acid					213:216	hydrochloric acid	200:216	hydrochloric acid (Nano-CS/Clino@H)	200:234	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	7	2	theme	Freundlich	979:988	arg1	models					1014:1019	The Dubinin-Radushkevich, Freundlich and Langmuir adsorption models	953:1019	The Dubinin-Radushkevich, Freundlich and Langmuir adsorption models	953:1019	The Dubinin-Radushkevich, Freundlich and Langmuir adsorption models were applied to describe the equilibrium isotherms.					
30807801	1	3	theme	@	313:313	arg1	PEHA					314:317	Nano-CS/Clino@PEHA	300:317	Nano-CS/Clino@PEHA	300:317	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	3	theme	@	313:313	arg1	pentaethylenehexamine					277:297	pentaethylenehexamine	277:297	pentaethylenehexamine (Nano-CS/Clino@PEHA)	277:318	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	4	with	acid	213:216	arg1	PEHA					314:317	Nano-CS/Clino@PEHA	300:317	Nano-CS/Clino@PEHA	300:317	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	4	with	acid	213:216	arg1	pentaethylenehexamine					277:297	pentaethylenehexamine	277:297	pentaethylenehexamine (Nano-CS/Clino@PEHA)	277:318	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	8	5	theme	experimental	1077:1088	arg1	data					1090:1093	The experimental data	1073:1093	The experimental data	1073:1093	The experimental data were also analyzed by first- and second-order and intra particle diffusion.					
30807801	2	6	theme	textural	385:392	arg1	properties					394:403	The textural properties	381:403	The textural properties of the Nano-CS/Clino nanocomposites	381:439	The textural properties of the Nano-CS/Clino nanocomposites were studied by various characterization techniques.					
30807801	4	7	theme	surface	619:625	arg1	0.9774					659:664	0.9774	659:664	0.9774	659:664	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	4	7	theme	surface	619:625	arg1	area					627:630	The BET specific surface area	602:630	The BET specific surface area of chitosan and Nano-CS	602:654	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	4	8	theme	Nano-CS	648:654	arg1	0.9774					659:664	0.9774	659:664	0.9774	659:664	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	4	8	theme	Nano-CS	648:654	arg1	area					627:630	The BET specific surface area	602:630	The BET specific surface area of chitosan and Nano-CS	602:654	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	8	9	theme	intra	1145:1149	arg1	diffusion					1160:1168	intra particle diffusion	1145:1168	intra particle diffusion	1145:1168	The experimental data were also analyzed by first- and second-order and intra particle diffusion.					
30807801	10	10	from	nitrate	1381:1387	arg1	presence					1403:1410	the presence	1399:1410	the presence of CO32-, SO42- and Cl- under competitive conditions,	1399:1464	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	9	11	theme	Thermodynamic	1171:1183	arg1	parameters					1185:1194	Thermodynamic parameters	1171:1194	Thermodynamic parameters studies	1171:1202	Thermodynamic parameters studies revealed that the nature of adsorption nitrate by nanocomposites synthesized is spontaneous and exothermic.					
30807801	10	12	theme	Cl->	1500:1503	arg1	SO42-					1505:1509	NO3- > CO32-> Cl-> SO42-	1486:1509	NO3- > CO32-> Cl-> SO42-	1486:1509	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	5	13	theme	nitrate	738:744	arg1	removal					727:733	the removal	723:733	the removal of nitrate	723:744	The effects of different parameters on the removal of nitrate were measured in detail.					
30807801	6	14	theme	prepared	775:782	arg1	composite					803:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	6	15	theme	Nano-CS/Clino	784:796	arg1	composite					803:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	9	16	theme	parameters	1185:1194	arg1	studies					1196:1202	Thermodynamic parameters studies	1171:1202	Thermodynamic parameters studies	1171:1202	Thermodynamic parameters studies revealed that the nature of adsorption nitrate by nanocomposites synthesized is spontaneous and exothermic.					
30807801	3	17	theme	gelation	531:538	arg1	technique					540:548	ionic gelation technique	525:548	ionic gelation technique that using tripolyphosphate as cross-linking agent	525:599	The Nano-CS was synthesized by ionic gelation technique that using tripolyphosphate as cross-linking agent.					
30807801	1	18	theme	@	232:232	arg1	H					233:233	Nano-CS/Clino@H	219:233	Nano-CS/Clino@H	219:233	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	18	theme	@	232:232	arg1	acid					213:216	hydrochloric acid	200:216	hydrochloric acid (Nano-CS/Clino@H)	200:234	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	0	19	theme	nitrate	78:84	arg1	removal					86:92	nitrate removal	78:92	nitrate removal from aqueous media	78:111	Characterization of functionalized chitosan-clinoptilolite nanocomposites for nitrate removal from aqueous media.					
30807801	1	20	used	used	329:332	arg2	Nano-CS/Clino					173:185	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino	114:185	Nano-CS/Clino	173:185	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	5	21	theme	different	699:707	arg1	parameters					709:718	different parameters	699:718	different parameters	699:718	The effects of different parameters on the removal of nitrate were measured in detail.					
30807801	6	22	theme	Nano-CS/Clino	878:890	arg1	H					892:892	Nano-CS/Clino@H	878:892	Nano-CS/Clino@H	878:892	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	10	23	theme	overall	1316:1322	arg1	tendency					1335:1342	The overall adsorption tendency	1312:1342	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions,	1312:1464	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	1	24	theme	Nano-CS/Clino	240:252	arg1	functionalization					254:270	Nano-CS/Clino functionalization	240:270	Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA)	240:318	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	25	theme	Nano-CS/Clino	219:231	arg1	H					233:233	Nano-CS/Clino@H	219:233	Nano-CS/Clino@H	219:233	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	25	theme	Nano-CS/Clino	219:231	arg1	acid					213:216	hydrochloric acid	200:216	hydrochloric acid (Nano-CS/Clino@H)	200:234	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	5	26	theme	parameters	709:718	arg1	effects					688:694	The effects	684:694	The effects of different parameters on the removal of nitrate	684:744	The effects of different parameters on the removal of nitrate were measured in detail.					
30807801	11	27	theme	Desorption	1512:1521	arg1	process					1523:1529	Desorption process	1512:1529	Desorption process	1512:1529	Desorption process was carried out with NaOH and proven it was an effective agent in the discharge of nitrate.					
30807801	2	28	theme	nanocomposites	426:439	arg1	properties					394:403	The textural properties	381:403	The textural properties of the Nano-CS/Clino nanocomposites	381:439	The textural properties of the Nano-CS/Clino nanocomposites were studied by various characterization techniques.					
30807801	10	29	dep	followed	1466:1473	arg1	SO42-					1505:1509	NO3- > CO32-> Cl-> SO42-	1486:1509	NO3- > CO32-> Cl-> SO42-	1486:1509	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	6	30	theme	277.77 mg/g	861:871	arg1	capacity					849:856	enhanced nitrate adsorption capacity	821:856	enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g	821:950	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	10	31	theme	competitive	1442:1452	arg1	conditions					1454:1463	competitive conditions	1442:1463	competitive conditions	1442:1463	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	2	32	theme	Nano-CS/Clino	412:424	arg1	nanocomposites					426:439	the Nano-CS/Clino nanocomposites	408:439	the Nano-CS/Clino nanocomposites	408:439	The textural properties of the Nano-CS/Clino nanocomposites were studied by various characterization techniques.					
30807801	0	33	theme	chitosan-clinoptilolite	35:57	arg1	Characterization					0:15	Characterization	0:15	Characterization of functionalized chitosan-clinoptilolite	0:57	Characterization of functionalized chitosan-clinoptilolite nanocomposites for nitrate removal from aqueous media.					
30807801	6	34	theme	@	891:891	arg1	H					892:892	Nano-CS/Clino@H	878:892	Nano-CS/Clino@H	878:892	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	3	35	theme	ionic	525:529	arg1	technique					540:548	ionic gelation technique	525:548	ionic gelation technique that using tripolyphosphate as cross-linking agent	525:599	The Nano-CS was synthesized by ionic gelation technique that using tripolyphosphate as cross-linking agent.					
30807801	0	36	theme	functionalized	20:33	arg1	chitosan-clinoptilolite					35:57	functionalized chitosan-clinoptilolite	20:57	functionalized chitosan-clinoptilolite	20:57	Characterization of functionalized chitosan-clinoptilolite nanocomposites for nitrate removal from aqueous media.					
30807801	1	37	with	functionalization	254:270	arg1	PEHA					314:317	Nano-CS/Clino@PEHA	300:317	Nano-CS/Clino@PEHA	300:317	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	37	with	functionalization	254:270	arg1	pentaethylenehexamine					277:297	pentaethylenehexamine	277:297	pentaethylenehexamine (Nano-CS/Clino@PEHA)	277:318	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	3	38	theme	cross-linking	581:593	arg1	agent					595:599	cross-linking agent	581:599	cross-linking agent	581:599	The Nano-CS was synthesized by ionic gelation technique that using tripolyphosphate as cross-linking agent.					
30807801	7	39	theme	equilibrium	1050:1060	arg1	isotherms					1062:1070	the equilibrium isotherms	1046:1070	the equilibrium isotherms	1046:1070	The Dubinin-Radushkevich, Freundlich and Langmuir adsorption models were applied to describe the equilibrium isotherms.					
30807801	2	40	theme	characterization	465:480	arg1	techniques					482:491	various characterization techniques	457:491	various characterization techniques	457:491	The textural properties of the Nano-CS/Clino nanocomposites were studied by various characterization techniques.					
30807801	4	41	theme	chitosan	635:642	arg1	0.9774					659:664	0.9774	659:664	0.9774	659:664	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	4	41	theme	chitosan	635:642	arg1	area					627:630	The BET specific surface area	602:630	The BET specific surface area of chitosan and Nano-CS	602:654	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	0	42	from	media	107:111	arg1	removal					86:92	nitrate removal	78:92	nitrate removal from aqueous media	78:111	Characterization of functionalized chitosan-clinoptilolite nanocomposites for nitrate removal from aqueous media.					
30807801	9	43	theme	adsorption	1232:1241	arg1	nitrate					1243:1249	adsorption nitrate	1232:1249	adsorption nitrate	1232:1249	Thermodynamic parameters studies revealed that the nature of adsorption nitrate by nanocomposites synthesized is spontaneous and exothermic.					
30807801	2	44	theme	various	457:463	arg1	techniques					482:491	various characterization techniques	457:491	various characterization techniques	457:491	The textural properties of the Nano-CS/Clino nanocomposites were studied by various characterization techniques.					
30807801	4	45	theme	BET	606:608	arg1	0.9774					659:664	0.9774	659:664	0.9774	659:664	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	4	45	theme	BET	606:608	arg1	area					627:630	The BET specific surface area	602:630	The BET specific surface area of chitosan and Nano-CS	602:654	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	10	46	theme	synthesized	1347:1357	arg1	nanocomposites					1359:1372	synthesized nanocomposites	1347:1372	synthesized nanocomposites	1347:1372	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	9	47	theme	nitrate	1243:1249	arg1	nature					1222:1227	the nature	1218:1227	the nature of adsorption nitrate by nanocomposites synthesized	1218:1279	Thermodynamic parameters studies revealed that the nature of adsorption nitrate by nanocomposites synthesized is spontaneous and exothermic.					
30807801	9	47	theme	nitrate	1243:1249	arg1	spontaneous					1284:1294	spontaneous	1284:1294	spontaneous	1284:1294	Thermodynamic parameters studies revealed that the nature of adsorption nitrate by nanocomposites synthesized is spontaneous and exothermic.					
30807801	1	48	theme	nitrate	348:354	arg1	ions					356:359	nitrate ions	348:359	nitrate ions	348:359	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	11	49	theme	effective	1578:1586	arg1	it					1568:1569	it	1568:1569	it	1568:1569	Desorption process was carried out with NaOH and proven it was an effective agent in the discharge of nitrate.					
30807801	11	49	theme	effective	1578:1586	arg1	agent					1588:1592	an effective agent	1575:1592	an effective agent in the discharge of nitrate	1575:1620	Desorption process was carried out with NaOH and proven it was an effective agent in the discharge of nitrate.					
30807801	11	50	from	agent	1588:1592	arg1	discharge					1601:1609	the discharge	1597:1609	the discharge of nitrate	1597:1620	Desorption process was carried out with NaOH and proven it was an effective agent in the discharge of nitrate.					
30807801	6	51	theme	adsorption	838:847	arg1	capacity					849:856	enhanced nitrate adsorption capacity	821:856	enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g	821:950	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	11	52	theme	nitrate	1614:1620	arg1	discharge					1601:1609	the discharge	1597:1609	the discharge of nitrate	1597:1620	Desorption process was carried out with NaOH and proven it was an effective agent in the discharge of nitrate.					
30807801	1	53	from	media	374:378	arg1	removal					337:343	removal	337:343	removal of nitrate ions from aqueous media	337:378	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	54	theme	ions	356:359	arg1	removal					337:343	removal	337:343	removal of nitrate ions from aqueous media	337:378	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	8	55	theme	particle	1151:1158	arg1	diffusion					1160:1168	intra particle diffusion	1145:1168	intra particle diffusion	1145:1168	The experimental data were also analyzed by first- and second-order and intra particle diffusion.					
30807801	6	56	theme	nitrate	830:836	arg1	capacity					849:856	enhanced nitrate adsorption capacity	821:856	enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g	821:950	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	1	57	theme	composite	162:170	arg1	Nano-CS/Clino					173:185	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino	114:185	Nano-CS/Clino	173:185	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	7	58	theme	adsorption	1003:1012	arg1	models					1014:1019	The Dubinin-Radushkevich, Freundlich and Langmuir adsorption models	953:1019	The Dubinin-Radushkevich, Freundlich and Langmuir adsorption models	953:1019	The Dubinin-Radushkevich, Freundlich and Langmuir adsorption models were applied to describe the equilibrium isotherms.					
30807801	4	59	theme	specific	610:617	arg1	0.9774					659:664	0.9774	659:664	0.9774	659:664	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	4	59	theme	specific	610:617	arg1	area					627:630	The BET specific surface area	602:630	The BET specific surface area of chitosan and Nano-CS	602:654	The BET specific surface area of chitosan and Nano-CS is 0.9774 and 52.8850 m2/g.					
30807801	6	60	theme	enhanced	821:828	arg1	capacity					849:856	enhanced nitrate adsorption capacity	821:856	enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g	821:950	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	10	61	theme	adsorption	1324:1333	arg1	tendency					1335:1342	The overall adsorption tendency	1312:1342	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions,	1312:1464	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	6	62	contain	possess	813:819	arg1	composite					803:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	6	62	contain	possess	813:819	arg2	capacity					849:856	enhanced nitrate adsorption capacity	821:856	enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g	821:950	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	10	63	theme	nanocomposites	1359:1372	arg1	tendency					1335:1342	The overall adsorption tendency	1312:1342	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions,	1312:1464	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	10	64	theme	CO32-	1415:1419	arg1	presence					1403:1410	the presence	1399:1410	the presence of CO32-, SO42- and Cl- under competitive conditions,	1399:1464	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	0	65	theme	aqueous	99:105	arg1	media					107:111	aqueous media	99:111	aqueous media	99:111	Characterization of functionalized chitosan-clinoptilolite nanocomposites for nitrate removal from aqueous media.					
30807801	10	66	theme	NO3- > CO32->	1486:1498	arg1	SO42-					1505:1509	NO3- > CO32-> Cl-> SO42-	1486:1509	NO3- > CO32-> Cl-> SO42-	1486:1509	The overall adsorption tendency of synthesized nanocomposites toward nitrate (NO3-) in the presence of CO32-, SO42- and Cl- under competitive conditions, followed the order: NO3- > CO32-> Cl-> SO42-.					
30807801	6	67	dep	capacity	849:856	arg1	found					917:921	found	917:921	were found to be 227.27 and 185.18 mg/g	912:950	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	7	68	theme	Langmuir	994:1001	arg1	adsorption					1003:1012	Langmuir adsorption	994:1012	Langmuir adsorption	994:1012	The Dubinin-Radushkevich, Freundlich and Langmuir adsorption models were applied to describe the equilibrium isotherms.					
30807801	1	69	theme	aqueous	366:372	arg1	media					374:378	aqueous media	366:378	aqueous media	366:378	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	6	70	theme	PEHA	798:801	arg1	composite					803:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite	771:811	The prepared Nano-CS/Clino@PEHA composite possess enhanced nitrate adsorption capacity of 277.77 mg/g than Nano-CS/Clino@H and Nano-CS/Clino were found to be 227.27 and 185.18 mg/g.					
30807801	5	71	from	effects	688:694	arg1	removal					727:733	the removal	723:733	the removal of nitrate	723:744	The effects of different parameters on the removal of nitrate were measured in detail.					
30807801	1	72	theme	Nano-CS/Clino	300:312	arg1	PEHA					314:317	Nano-CS/Clino@PEHA	300:317	Nano-CS/Clino@PEHA	300:317	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
30807801	1	72	theme	Nano-CS/Clino	300:312	arg1	pentaethylenehexamine					277:297	pentaethylenehexamine	277:297	pentaethylenehexamine (Nano-CS/Clino@PEHA)	277:318	The nanochitosan/clinoptilolite (Nano-CS/Clino) composite, Nano-CS/Clino activated by hydrochloric acid (Nano-CS/Clino@H) and Nano-CS/Clino functionalization with pentaethylenehexamine (Nano-CS/Clino@PEHA) has been used to removal of nitrate ions from aqueous media.					
31292505	0	0	theme	rice	68:71	arg1	quality					57:63	quality	57:63	quality of rice	57:71	Effect of different shading materials on grain yield and quality of rice.					
31292505	0	0	theme	rice	68:71	arg1	yield					47:51	grain yield	41:51	grain yield	41:51	Effect of different shading materials on grain yield and quality of rice.					
31292505	8	1	theme	light	1418:1422	arg1	environment					1424:1434	the light environment	1414:1434	the light environment	1414:1434	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions will be better than black net.					
31292505	3	2	theme	different	611:619	arg1	effects					621:627	different effects	611:627	different effects	611:627	Yield decreased under Shading (W) (15.3-17.7%) and Shading (B) (16.6-20.0%) compared to under sunny day (CK), and different effects on rice quality, which is mainly affected by changes in light quality, were observed.					
31292505	1	3	theme	different	288:296	arg1	compositions					298:309	different compositions	288:309	different compositions	288:309	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	8	4	theme	rice	1439:1442	arg1	growth					1444:1449	rice growth	1439:1449	rice growth	1439:1449	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions will be better than black net.					
31292505	5	5	theme	cloudy	954:959	arg1	condition					965:973	the cloudy day condition	950:973	the cloudy day condition	950:973	Red light composition under Shading (W) was closer to that of the cloudy day condition.					
31292505	4	6	from	change	719:724	arg1	quality					735:741	light quality	729:741	light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W)	729:811	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	2	7	dep	nylon	438:442	arg1	B					458:458	B	458:458	B	458:458	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	7	dep	nylon	438:442	arg1	Shading					449:455	Shading	449:455	Shading (B)	449:459	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	1	8	theme	compositions	298:309	arg1	quality					229:235	light quality	223:235	light quality	223:235	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	3	9	from	effects	621:627	arg1	quality					637:643	rice quality	632:643	rice quality	632:643	Yield decreased under Shading (W) (15.3-17.7%) and Shading (B) (16.6-20.0%) compared to under sunny day (CK), and different effects on rice quality, which is mainly affected by changes in light quality, were observed.					
31292505	5	10	theme	day	961:963	arg1	condition					965:973	the cloudy day condition	950:973	the cloudy day condition	950:973	Red light composition under Shading (W) was closer to that of the cloudy day condition.					
31292505	4	11	theme	R/FR	765:768	arg1	proportions					770:780	R/FR proportions	765:780	R/FR proportions	765:780	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	8	12	theme	shading	1342:1348	arg1	material					1350:1357	the shading material	1338:1357	the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions	1338:1473	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions will be better than black net.					
31292505	6	13	theme	light	999:1003	arg1	lower					1041:1045	lower	1041:1045	lower	1041:1045	The proportion of blue light under Shading (W) was significantly lower than that under CK conditions; under Shading (B), it was higher than that under all conditions.					
31292505	6	13	theme	light	999:1003	arg1	proportion					980:989	The proportion	976:989	The proportion of blue light under Shading (W)	976:1021	The proportion of blue light under Shading (W) was significantly lower than that under CK conditions; under Shading (B), it was higher than that under all conditions.					
31292505	3	14	theme	light	685:689	arg1	quality					691:697	light quality	685:697	light quality	685:697	Yield decreased under Shading (W) (15.3-17.7%) and Shading (B) (16.6-20.0%) compared to under sunny day (CK), and different effects on rice quality, which is mainly affected by changes in light quality, were observed.					
31292505	8	15	from	influence	1401:1409	arg1	growth					1444:1449	rice growth	1439:1449	rice growth	1439:1449	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions will be better than black net.					
31292505	0	16	theme	grain	41:45	arg1	yield					47:51	grain yield	41:51	grain yield	41:51	Effect of different shading materials on grain yield and quality of rice.					
31292505	2	17	theme	shading	377:383	arg1	materials					385:393	shading materials	377:393	shading materials	377:393	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	18	theme	white	396:400	arg1	yarn					409:412	white cotton yarn	396:412	white cotton yarn (Shading (W))	396:426	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	7	19	from	differences	1147:1157	arg1	quality					1168:1174	light quality	1162:1174	light quality	1162:1174	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	2	20	theme	cloudy	484:489	arg1	days					491:494	cloudy days	484:494	cloudy days	484:494	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	5	21	theme	Red	888:890	arg1	composition					898:908	Red light composition	888:908	Red light composition under Shading (W)	888:926	Red light composition under Shading (W) was closer to that of the cloudy day condition.					
31292505	4	22	theme	Shading	864:870	arg1	conditions					876:885	CK and Shading (B) conditions	857:885	CK and Shading (B) conditions	857:885	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	1	23	theme	light	223:227	arg1	quality					229:235	light quality	223:235	light quality	223:235	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	7	24	theme	rice	1303:1306	arg1	quality					1308:1314	poor rice quality	1298:1314	poor rice quality	1298:1314	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	2	25	theme	rice	330:333	arg1	Oryza					346:350	Oryza	346:350	Oryza	346:350	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	25	theme	rice	330:333	arg1	varieties					335:343	four different rice varieties	315:343	four different rice varieties (Oryza sativa L.)	315:361	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	0	26	theme	shading	20:26	arg1	materials					28:36	different shading materials	10:36	different shading materials	10:36	Effect of different shading materials on grain yield and quality of rice.					
31292505	5	27	theme	light	892:896	arg1	composition					898:908	Red light composition	888:908	Red light composition under Shading (W)	888:926	Red light composition under Shading (W) was closer to that of the cloudy day condition.					
31292505	0	28	theme	different	10:18	arg1	materials					28:36	different shading materials	10:36	different shading materials	10:36	Effect of different shading materials on grain yield and quality of rice.					
31292505	7	29	theme	final	1216:1220	arg1	synthesis					1229:1237	final starch synthesis	1216:1237	final starch synthesis	1216:1237	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	2	30	theme	net	444:446	arg1	nylon					438:442	black nylon net	432:446	black nylon net (Shading (B))	432:460	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	7	31	from	synthesis	1229:1237	arg1	leaves					1205:1210	leaves	1205:1210	leaves	1205:1210	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	4	32	from	that	846:849	arg1	change					719:724	The change	715:724	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W)	715:811	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	4	32	from	that	846:849	arg1	different					831:839	different	831:839	different	831:839	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	2	33	theme	cotton	402:407	arg1	yarn					409:412	white cotton yarn	396:412	white cotton yarn (Shading (W))	396:426	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	4	34	theme	CK	857:858	arg1	conditions					876:885	CK and Shading (B) conditions	857:885	CK and Shading (B) conditions	857:885	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	7	35	from	photosynthesis	1187:1200	arg1	leaves					1205:1210	leaves	1205:1210	leaves	1205:1210	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	7	36	theme	poor	1298:1301	arg1	quality					1308:1314	poor rice quality	1298:1314	poor rice quality	1298:1314	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	0	37	theme	materials	28:36	arg1	Effect					0:5	Effect	0:5	Effect of different shading materials on grain yield and quality of rice.	0:72	Effect of different shading materials on grain yield and quality of rice.					
31292505	7	38	theme	chalky	1263:1268	arg1	rate					1276:1279	increased chalky grain rate	1253:1279	increased chalky grain rate	1253:1279	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	2	39	theme	black	432:436	arg1	nylon					438:442	black nylon net	432:446	black nylon net (Shading (B))	432:460	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	7	40	theme	starch	1222:1227	arg1	synthesis					1229:1237	final starch synthesis	1216:1237	final starch synthesis	1216:1237	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	7	41	theme	increased	1253:1261	arg1	rate					1276:1279	increased chalky grain rate	1253:1279	increased chalky grain rate	1253:1279	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	3	42	theme	sunny	591:595	arg1	CK					602:603	CK	602:603	CK	602:603	Yield decreased under Shading (W) (15.3-17.7%) and Shading (B) (16.6-20.0%) compared to under sunny day (CK), and different effects on rice quality, which is mainly affected by changes in light quality, were observed.					
31292505	3	42	theme	sunny	591:595	arg1	day					597:599	sunny day	591:599	sunny day (CK)	591:604	Yield decreased under Shading (W) (15.3-17.7%) and Shading (B) (16.6-20.0%) compared to under sunny day (CK), and different effects on rice quality, which is mainly affected by changes in light quality, were observed.					
31292505	6	43	theme	CK	1063:1064	arg1	conditions					1066:1075	CK conditions	1063:1075	CK conditions	1063:1075	The proportion of blue light under Shading (W) was significantly lower than that under CK conditions; under Shading (B), it was higher than that under all conditions.					
31292505	6	44	theme	blue	994:997	arg1	light					999:1003	blue light	994:1003	blue light	994:1003	The proportion of blue light under Shading (W) was significantly lower than that under CK conditions; under Shading (B), it was higher than that under all conditions.					
31292505	1	45	theme	basic	85:89	arg1	factor					105:110	a basic environmental factor	83:110	a basic environmental factor required for plant growth and development	83:152	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	1	45	theme	basic	85:89	arg1	Light					74:78	Light	74:78	Light	74:78	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	0	46	from	Effect	0:5	arg1	quality					57:63	quality	57:63	quality of rice	57:71	Effect of different shading materials on grain yield and quality of rice.					
31292505	0	46	from	Effect	0:5	arg1	yield					47:51	grain yield	41:51	grain yield	41:51	Effect of different shading materials on grain yield and quality of rice.					
31292505	2	47	used	used	467:470	arg2	yarn					409:412	white cotton yarn	396:412	white cotton yarn (Shading (W))	396:426	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	47	used	used	467:470	arg2	nylon					438:442	black nylon net	432:446	black nylon net (Shading (B))	432:460	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	47	used	used	467:470	arg2	kinds					368:372	two kinds	364:372	two kinds of shading materials	364:393	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	48	dep	yarn	409:412	arg1	Shading					415:421	Shading	415:421	Shading (W)	415:425	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	48	dep	yarn	409:412	arg1	W					424:424	W	424:424	W	424:424	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	49	theme	materials	385:393	arg1	yarn					409:412	white cotton yarn	396:412	white cotton yarn (Shading (W))	396:426	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	49	theme	materials	385:393	arg1	kinds					368:372	two kinds	364:372	two kinds of shading materials	364:393	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	49	theme	materials	385:393	arg1	nylon					438:442	black nylon net	432:446	black nylon net (Shading (B))	432:460	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	8	50	theme	cloudy	1457:1462	arg1	conditions					1464:1473	cloudy conditions	1457:1473	cloudy conditions	1457:1473	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions will be better than black net.					
31292505	1	51	theme	environmental	91:103	arg1	factor					105:110	a basic environmental factor	83:110	a basic environmental factor required for plant growth and development	83:152	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	1	51	theme	environmental	91:103	arg1	Light					74:78	Light	74:78	Light	74:78	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	1	52	theme	different	262:270	arg1	wavelengths					272:282	different wavelengths	262:282	different wavelengths	262:282	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	4	53	dep	quality	735:741	arg1	Red					757:759	Red	757:759	Red	757:759	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	4	53	dep	quality	735:741	arg1	proportions					770:780	R/FR proportions	765:780	R/FR proportions	765:780	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	4	53	dep	quality	735:741	arg1	Green					750:754	Green	750:754	Green	750:754	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	4	53	dep	quality	735:741	arg1	Blue					744:747	Blue	744:747	Blue	744:747	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	7	54	theme	light	1162:1166	arg1	quality					1168:1174	light quality	1162:1174	light quality	1162:1174	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
31292505	3	55	from	changes	674:680	arg1	quality					691:697	light quality	685:697	light quality	685:697	Yield decreased under Shading (W) (15.3-17.7%) and Shading (B) (16.6-20.0%) compared to under sunny day (CK), and different effects on rice quality, which is mainly affected by changes in light quality, were observed.					
31292505	8	56	theme	cotton	1323:1328	arg1	yarn					1330:1333	White cotton yarn	1317:1333	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions	1317:1473	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions will be better than black net.					
31292505	2	57	dep	Oryza	346:350	arg1	L.					359:360	Oryza sativa L.	346:360	Oryza sativa L.	346:360	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	58	theme	different	320:328	arg1	Oryza					346:350	Oryza	346:350	Oryza	346:350	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	2	58	theme	different	320:328	arg1	varieties					335:343	four different rice varieties	315:343	four different rice varieties (Oryza sativa L.)	315:361	In four different rice varieties (Oryza sativa L.), two kinds of shading materials, white cotton yarn (Shading (W)) and black nylon net (Shading (B)) were used to simulate cloudy days.					
31292505	3	59	theme	rice	632:635	arg1	quality					637:643	rice quality	632:643	rice quality	632:643	Yield decreased under Shading (W) (15.3-17.7%) and Shading (B) (16.6-20.0%) compared to under sunny day (CK), and different effects on rice quality, which is mainly affected by changes in light quality, were observed.					
31292505	1	60	theme	light	195:199	arg1	quantity					201:208	light quantity	195:208	light quantity	195:208	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	4	61	theme	light	729:733	arg1	quality					735:741	light quality	729:741	light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W)	729:811	The change in light quality (Blue, Green, Red and R/FR proportions) represented under Shading (W) was significantly different from that under CK and Shading (B) conditions.					
31292505	1	62	theme	wavelengths	272:282	arg1	quality					229:235	light quality	223:235	light quality	223:235	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	8	63	theme	further	1363:1369	arg1	research					1371:1378	further research	1363:1378	further research used to simulate the influence of the light environment on rice growth under cloudy conditions	1363:1473	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions will be better than black net.					
31292505	8	64	theme	environment	1424:1434	arg1	influence					1401:1409	the influence	1397:1409	the influence of the light environment on rice growth	1397:1449	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions will be better than black net.					
31292505	1	65	theme	plant	125:129	arg1	growth					131:136	plant growth	125:136	plant growth	125:136	Light is a basic environmental factor required for plant growth and development; however, these are not only affected by light quantity, but also by light quality - light and radiation of different wavelengths and different compositions.					
31292505	8	66	theme	White	1317:1321	arg1	yarn					1330:1333	White cotton yarn	1317:1333	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions	1317:1473	White cotton yarn as the shading material for further research used to simulate the influence of the light environment on rice growth under cloudy conditions will be better than black net.					
31292505	7	67	theme	grain	1270:1274	arg1	rate					1276:1279	increased chalky grain rate	1253:1279	increased chalky grain rate	1253:1279	The differences in light quality may affect photosynthesis in leaves and final starch synthesis, resulting in increased chalky grain rate, chalkiness, and poor rice quality.					
29482129	5	0	theme	Porous	786:791	arg1	granules					800:807	Porous silica granules	786:807	Porous silica granules	786:807	Porous silica granules, having surface area of 157 m2/g, were obtained after removal of carbon black by calcination.					
29482129	2	1	theme	stabilizing	415:425	arg1	arabic					403:408	gum arabic	399:408	gum arabic	399:408	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	2	1	theme	stabilizing	415:425	arg1	agent					427:431	a stabilizing agent	413:431	a stabilizing agent	413:431	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	6	2	theme	absorbing	933:941	arg1	coating					943:949	An environment-friendly solar absorbing coating	903:949	An environment-friendly solar absorbing coating	903:949	An environment-friendly solar absorbing coating had been prepared using as synthesized granules.					
29482129	6	3	theme	solar	927:931	arg1	coating					943:949	An environment-friendly solar absorbing coating	903:949	An environment-friendly solar absorbing coating	903:949	An environment-friendly solar absorbing coating had been prepared using as synthesized granules.					
29482129	3	4	from	stability	534:542	arg1	morphology					577:586	the morphology	573:586	the morphology of the spray dried granules	573:614	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	2	5	theme	hydrophilic	305:315	arg1	particles					324:332	hydrophilic silica particles	305:332	hydrophilic silica particles	305:332	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	3	6	from	effect	563:568	arg1	morphology					577:586	the morphology	573:586	the morphology of the spray dried granules	573:614	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	2	7	theme	water	341:345	arg1	droplets					347:354	the water droplets	337:354	the water droplets	337:354	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	1	8	theme	Spray	74:78	arg1	drying					80:85	Spray drying	74:85	Spray drying	74:85	Spray drying had been used to synthesize silica-carbon black nanocomposite micrometric granules with a uniform distribution of the two components.					
29482129	1	9	theme	uniform	177:183	arg1	distribution					185:196	a uniform distribution	175:196	a uniform distribution of the two components	175:218	Spray drying had been used to synthesize silica-carbon black nanocomposite micrometric granules with a uniform distribution of the two components.					
29482129	2	10	theme	hydrophobic	282:292	arg1	carbon					294:299	hydrophobic carbon	282:299	hydrophobic carbon	282:299	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	4	11	theme	electron	665:672	arg1	microscopy					674:683	electron microscopy	665:683	electron microscopy	665:683	X-ray and neutron scattering, complemented with electron microscopy, were used to investigate the correlation and distribution of the nanoparticles within the granules.					
29482129	0	12	theme	assisted	11:18	arg1	synthesis					20:28	Biopolymer assisted synthesis	0:28	Biopolymer assisted synthesis of silica-carbon composite by spray drying.	0:72	Biopolymer assisted synthesis of silica-carbon composite by spray drying.					
29482129	5	13	theme	silica	793:798	arg1	granules					800:807	Porous silica granules	786:807	Porous silica granules	786:807	Porous silica granules, having surface area of 157 m2/g, were obtained after removal of carbon black by calcination.					
29482129	4	14	theme	nanoparticles	751:763	arg1	distribution					731:742	distribution	731:742	distribution	731:742	X-ray and neutron scattering, complemented with electron microscopy, were used to investigate the correlation and distribution of the nanoparticles within the granules.					
29482129	4	14	theme	nanoparticles	751:763	arg1	correlation					715:725	correlation	715:725	correlation	715:725	X-ray and neutron scattering, complemented with electron microscopy, were used to investigate the correlation and distribution of the nanoparticles within the granules.					
29482129	3	15	theme	sol	551:553	arg1	stability					534:542	the stability	530:542	the stability of the sol	530:553	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	3	15	theme	sol	551:553	arg1	effect					563:568	its effect	559:568	its effect on the morphology of the spray dried granules	559:614	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	5	16	theme	black	881:885	arg1	carbon					874:879	carbon black	874:885	carbon black	874:885	Porous silica granules, having surface area of 157 m2/g, were obtained after removal of carbon black by calcination.					
29482129	0	17	theme	Biopolymer	0:9	arg1	synthesis					20:28	Biopolymer assisted synthesis	0:28	Biopolymer assisted synthesis of silica-carbon composite by spray drying.	0:72	Biopolymer assisted synthesis of silica-carbon composite by spray drying.					
29482129	4	18	used	used	691:694	arg2	scattering					635:644	neutron scattering	627:644	neutron scattering	627:644	X-ray and neutron scattering, complemented with electron microscopy, were used to investigate the correlation and distribution of the nanoparticles within the granules.					
29482129	4	18	used	used	691:694	arg2	X-ray					617:621	X-ray	617:621	X-ray	617:621	X-ray and neutron scattering, complemented with electron microscopy, were used to investigate the correlation and distribution of the nanoparticles within the granules.					
29482129	6	19	theme	synthesized	978:988	arg1	granules					990:997	synthesized granules	978:997	synthesized granules	978:997	An environment-friendly solar absorbing coating had been prepared using as synthesized granules.					
29482129	5	20	theme	157 m2/g	833:840	arg1	area					825:828	surface area	817:828	surface area of 157 m2/g	817:840	Porous silica granules, having surface area of 157 m2/g, were obtained after removal of carbon black by calcination.					
29482129	2	21	theme	particles	324:332	arg1	diffusion					269:277	the preferential diffusion	252:277	the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets	252:354	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	3	22	theme	dried	601:605	arg1	granules					607:614	the spray dried granules	591:614	the spray dried granules	591:614	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	2	23	theme	former	445:450	arg1	network					437:443	network former	437:450	network former	437:450	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	2	24	theme	carbon	294:299	arg1	diffusion					269:277	the preferential diffusion	252:277	the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets	252:354	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	2	25	theme	silica	317:322	arg1	particles					324:332	hydrophilic silica particles	305:332	hydrophilic silica particles	305:332	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	1	26	theme	components	209:218	arg1	distribution					185:196	a uniform distribution	175:196	a uniform distribution of the two components	175:218	Spray drying had been used to synthesize silica-carbon black nanocomposite micrometric granules with a uniform distribution of the two components.					
29482129	0	27	theme	composite	47:55	arg1	synthesis					20:28	Biopolymer assisted synthesis	0:28	Biopolymer assisted synthesis of silica-carbon composite by spray drying.	0:72	Biopolymer assisted synthesis of silica-carbon composite by spray drying.					
29482129	1	28	theme	silica-carbon	115:127	arg1	granules					161:168	silica-carbon black nanocomposite micrometric granules	115:168	silica-carbon black nanocomposite micrometric granules	115:168	Spray drying had been used to synthesize silica-carbon black nanocomposite micrometric granules with a uniform distribution of the two components.					
29482129	3	29	used	used	516:519	arg2	nanoparticles					497:509	Both positive and negatively charged silica nanoparticles	453:509	Both positive and negatively charged silica nanoparticles	453:509	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	2	30	from	diffusion	269:277	arg1	droplets					347:354	the water droplets	337:354	the water droplets	337:354	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	0	31	theme	silica-carbon	33:45	arg1	composite					47:55	silica-carbon composite	33:55	silica-carbon composite	33:55	Biopolymer assisted synthesis of silica-carbon composite by spray drying.					
29482129	3	32	theme	granules	607:614	arg1	morphology					577:586	the morphology	573:586	the morphology of the spray dried granules	573:614	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	3	33	theme	charged	482:488	arg1	nanoparticles					497:509	Both positive and negatively charged silica nanoparticles	453:509	Both positive and negatively charged silica nanoparticles	453:509	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	0	34	theme	spray	60:64	arg1	drying					66:71	spray drying	60:71	spray drying	60:71	Biopolymer assisted synthesis of silica-carbon composite by spray drying.					
29482129	3	35	theme	positive	458:465	arg1	nanoparticles					497:509	Both positive and negatively charged silica nanoparticles	453:509	Both positive and negatively charged silica nanoparticles	453:509	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	5	36	contain	having	810:815	arg1	granules					800:807	Porous silica granules	786:807	Porous silica granules	786:807	Porous silica granules, having surface area of 157 m2/g, were obtained after removal of carbon black by calcination.					
29482129	5	36	contain	having	810:815	arg2	area					825:828	surface area	817:828	surface area of 157 m2/g	817:840	Porous silica granules, having surface area of 157 m2/g, were obtained after removal of carbon black by calcination.					
29482129	2	37	theme	evaporative	363:373	arg1	assembly					375:382	evaporative assembly	363:382	evaporative assembly	363:382	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	5	38	theme	surface	817:823	arg1	area					825:828	surface area	817:828	surface area of 157 m2/g	817:840	Porous silica granules, having surface area of 157 m2/g, were obtained after removal of carbon black by calcination.					
29482129	3	39	theme	spray	595:599	arg1	granules					607:614	the spray dried granules	591:614	the spray dried granules	591:614	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	1	40	used	used	96:99	arg2	drying					80:85	Spray drying	74:85	Spray drying	74:85	Spray drying had been used to synthesize silica-carbon black nanocomposite micrometric granules with a uniform distribution of the two components.					
29482129	1	41	theme	micrometric	149:159	arg1	granules					161:168	silica-carbon black nanocomposite micrometric granules	115:168	silica-carbon black nanocomposite micrometric granules	115:168	Spray drying had been used to synthesize silica-carbon black nanocomposite micrometric granules with a uniform distribution of the two components.					
29482129	1	42	theme	black	129:133	arg1	granules					161:168	silica-carbon black nanocomposite micrometric granules	115:168	silica-carbon black nanocomposite micrometric granules	115:168	Spray drying had been used to synthesize silica-carbon black nanocomposite micrometric granules with a uniform distribution of the two components.					
29482129	1	43	theme	nanocomposite	135:147	arg1	granules					161:168	silica-carbon black nanocomposite micrometric granules	115:168	silica-carbon black nanocomposite micrometric granules	115:168	Spray drying had been used to synthesize silica-carbon black nanocomposite micrometric granules with a uniform distribution of the two components.					
29482129	2	44	theme	preferential	256:267	arg1	diffusion					269:277	the preferential diffusion	252:277	the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets	252:354	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	3	45	theme	silica	490:495	arg1	nanoparticles					497:509	Both positive and negatively charged silica nanoparticles	453:509	Both positive and negatively charged silica nanoparticles	453:509	Both positive and negatively charged silica nanoparticles were used to check the stability of the sol and its effect on the morphology of the spray dried granules.					
29482129	2	46	theme	gum	399:401	arg1	arabic					403:408	gum arabic	399:408	gum arabic	399:408	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	2	46	theme	gum	399:401	arg1	agent					427:431	a stabilizing agent	413:431	a stabilizing agent	413:431	This was achieved by hindering the preferential diffusion of hydrophobic carbon and hydrophilic silica particles in the water droplets during evaporative assembly by introducing gum arabic as a stabilizing agent and network former.					
29482129	5	47	theme	carbon	874:879	arg1	removal					863:869	removal	863:869	removal of carbon black by calcination	863:900	Porous silica granules, having surface area of 157 m2/g, were obtained after removal of carbon black by calcination.					
29482129	4	48	theme	neutron	627:633	arg1	scattering					635:644	neutron scattering	627:644	neutron scattering	627:644	X-ray and neutron scattering, complemented with electron microscopy, were used to investigate the correlation and distribution of the nanoparticles within the granules.					
30712592	2	0	theme	solid-phase	458:468	arg1	extraction					470:479	the magnetic solid-phase extraction	445:479	the magnetic solid-phase extraction (MSPE) of MCs	445:493	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	0	theme	solid-phase	458:468	arg1	MSPE					482:485	MSPE	482:485	MSPE	482:485	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	3	1	theme	trace	662:666	arg1	MCs					668:670	trace MCs	662:670	trace MCs in water samples	662:687	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	2	2	theme	Fe3O4	356:360	arg1	polymer					347:353	the magnetic γ-cyclodextrin polymer	319:353	the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite	319:381	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	2	theme	Fe3O4	356:360	arg1	γ-CDP					366:370	Fe3O4@PDA@γ-CDP	356:370	Fe3O4@PDA@γ-CDP	356:370	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	3	theme	magnetic	449:456	arg1	extraction					470:479	the magnetic solid-phase extraction	445:479	the magnetic solid-phase extraction (MSPE) of MCs	445:493	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	3	theme	magnetic	449:456	arg1	MSPE					482:485	MSPE	482:485	MSPE	482:485	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	3	4	theme	extraction	635:644	arg1	performance					646:656	an excellent extraction performance	622:656	an excellent extraction performance for trace MCs in water samples	622:687	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	2	5	theme	MCs	491:493	arg1	extraction					470:479	the magnetic solid-phase extraction	445:479	the magnetic solid-phase extraction (MSPE) of MCs	445:493	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	5	theme	MCs	491:493	arg1	MSPE					482:485	MSPE	482:485	MSPE	482:485	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	4	6	with	repeatability	949:961	arg1	RSDs					1002:1005	RSDs	1002:1005	RSDs	1002:1005	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	6	with	repeatability	949:961	arg1	deviations					990:999	the relative standard deviations	968:999	the relative standard deviations (RSDs) lower than 6.2% (n = 5)	968:1030	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	3	7	theme	prepared	500:507	arg1	microspheres					525:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	4	8	theme	standard	981:988	arg1	RSDs					1002:1005	RSDs	1002:1005	RSDs	1002:1005	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	8	theme	standard	981:988	arg1	deviations					990:999	the relative standard deviations	968:999	the relative standard deviations (RSDs) lower than 6.2% (n = 5)	968:1030	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	5	9	from	potential	1186:1194	arg1	lake					1232:1235	lake	1232:1235	lake	1232:1235	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	9	from	potential	1186:1194	arg1	water					1245:1249	sea water	1241:1249	sea water	1241:1249	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	0	10	theme	microcystins	102:113	arg1	extraction					88:97	the magnetic solid-phase extraction	63:97	the magnetic solid-phase extraction of microcystins in water samples	63:130	Magnetic γ-cyclodextrin polymer with compatible cavity promote the magnetic solid-phase extraction of microcystins in water samples.					
30712592	3	11	theme	water	675:679	arg1	samples					681:687	water samples	675:687	water samples	675:687	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	3	12	theme	Fe3O4	509:513	arg1	microspheres					525:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	2	13	theme	facile	274:279	arg1	strategy					281:288	a facile strategy	272:288	a facile strategy	272:288	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	5	14	theme	trace-level	1199:1209	arg1	determination					1215:1227	trace-level MCs determination	1199:1227	trace-level MCs determination	1199:1227	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	4	15	theme	low	884:886	arg1	1.0-1000 pg mL-1					866:881	1.0-1000 pg mL-1	866:881	1.0-1000 pg mL-1	866:881	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	15	theme	low	884:886	arg1	limits					888:893	low limits	884:893	low limits of detection (0.8-2.0 pg mL-1, S/N = 3)	884:933	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	16	theme	MCs	787:789	arg1	detection					774:782	detection	774:782	detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5)	774:1030	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	5	17	theme	MCs	1211:1213	arg1	determination					1215:1227	trace-level MCs determination	1199:1227	trace-level MCs determination	1199:1227	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	2	18	theme	composite	373:381	arg1	polymer					347:353	the magnetic γ-cyclodextrin polymer	319:353	the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite	319:381	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	18	theme	composite	373:381	arg1	γ-CDP					366:370	Fe3O4@PDA@γ-CDP	356:370	Fe3O4@PDA@γ-CDP	356:370	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	0	19	theme	water	118:122	arg1	samples					124:130	water samples	118:130	water samples	118:130	Magnetic γ-cyclodextrin polymer with compatible cavity promote the magnetic solid-phase extraction of microcystins in water samples.					
30712592	4	20	theme	satisfactory	936:947	arg1	repeatability					949:961	satisfactory repeatability	936:961	satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5)	936:1030	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	20	theme	satisfactory	936:947	arg1	1.0-1000 pg mL-1					866:881	1.0-1000 pg mL-1	866:881	1.0-1000 pg mL-1	866:881	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	21	theme	handy	718:722	arg1	method					749:754	a handy, sensitive and efficient method	716:754	a handy, sensitive and efficient method	716:754	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	5	22	theme	determination	1215:1227	arg1	potential					1186:1194	great potential	1180:1194	great potential of trace-level MCs determination in lake and sea water	1180:1249	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	3	23	dep	exhibit	538:544	arg1	contribute					608:617	contribute	608:617	contribute to an excellent extraction performance for trace MCs in water samples	608:687	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	0	24	theme	γ-cyclodextrin	9:22	arg1	polymer					24:30	Magnetic γ-cyclodextrin polymer	0:30	Magnetic γ-cyclodextrin polymer with compatible cavity	0:53	Magnetic γ-cyclodextrin polymer with compatible cavity promote the magnetic solid-phase extraction of microcystins in water samples.					
30712592	4	25	theme	detection	898:906	arg1	1.0-1000 pg mL-1					866:881	1.0-1000 pg mL-1	866:881	1.0-1000 pg mL-1	866:881	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	25	theme	detection	898:906	arg1	limits					888:893	low limits	884:893	low limits of detection (0.8-2.0 pg mL-1, S/N = 3)	884:933	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	26	theme	water	794:798	arg1	samples					800:806	water samples	794:806	water samples	794:806	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	3	27	theme	@	518:518	arg1	microspheres					525:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	4	28	dep	detection	898:906	arg1	S/N = 3					926:932	S/N = 3	926:932	S/N = 3	926:932	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	28	dep	detection	898:906	arg1	0.8-2.0 pg mL-1					909:923	0.8-2.0 pg mL-1	909:923	0.8-2.0 pg mL-1	909:923	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	1	29	theme	eruptive	175:182	arg1	cyanobacteria					184:196	eruptive cyanobacteria	175:196	eruptive cyanobacteria	175:196	Microcystins (MCs), which are produced by eruptive cyanobacteria, seriously threaten the health of humans for their poisonousness.					
30712592	0	30	theme	Magnetic	0:7	arg1	polymer					24:30	Magnetic γ-cyclodextrin polymer	0:30	Magnetic γ-cyclodextrin polymer with compatible cavity	0:53	Magnetic γ-cyclodextrin polymer with compatible cavity promote the magnetic solid-phase extraction of microcystins in water samples.					
30712592	3	31	theme	γ-CDP	519:523	arg1	microspheres					525:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	2	32	theme	@	365:365	arg1	polymer					347:353	the magnetic γ-cyclodextrin polymer	319:353	the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite	319:381	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	32	theme	@	365:365	arg1	γ-CDP					366:370	Fe3O4@PDA@γ-CDP	356:370	Fe3O4@PDA@γ-CDP	356:370	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	5	33	from	detection	1110:1118	arg1	water					1136:1140	fresh water	1130:1140	fresh water	1130:1140	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	33	from	detection	1110:1118	arg1	water					1150:1154	sea water	1146:1154	sea water	1146:1154	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	34	from	extraction	1095:1104	arg1	water					1136:1140	fresh water	1130:1140	fresh water	1130:1140	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	34	from	extraction	1095:1104	arg1	water					1150:1154	sea water	1146:1154	sea water	1146:1154	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	35	dep	water	1136:1140	arg1	samples					1156:1162	samples	1156:1162	samples	1156:1162	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	3	36	theme	@	514:514	arg1	microspheres					525:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	5	37	theme	great	1180:1184	arg1	potential					1186:1194	great potential	1180:1194	great potential of trace-level MCs determination in lake and sea water	1180:1249	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	3	38	theme	PDA	515:517	arg1	microspheres					525:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres	496:536	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	0	39	from	extraction	88:97	arg1	samples					124:130	water samples	118:130	water samples	118:130	Magnetic γ-cyclodextrin polymer with compatible cavity promote the magnetic solid-phase extraction of microcystins in water samples.					
30712592	3	40	theme	good	546:549	arg1	dispersibility					559:572	good aqueous dispersibility	546:572	good aqueous dispersibility	546:572	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	3	41	from	MCs	668:670	arg1	samples					681:687	water samples	675:687	water samples	675:687	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	0	42	theme	compatible	37:46	arg1	cavity					48:53	compatible cavity	37:53	compatible cavity	37:53	Magnetic γ-cyclodextrin polymer with compatible cavity promote the magnetic solid-phase extraction of microcystins in water samples.					
30712592	3	43	theme	aqueous	551:557	arg1	dispersibility					559:572	good aqueous dispersibility	546:572	good aqueous dispersibility	546:572	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	4	44	from	detection	774:782	arg1	samples					800:806	water samples	794:806	water samples	794:806	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	45	theme	1.0-1000 pg mL-1	866:881	arg1	range					857:861	the range	853:861	the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5)	853:1030	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	5	46	from	MCs	1123:1125	arg1	water					1136:1140	fresh water	1130:1140	fresh water	1130:1140	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	46	from	MCs	1123:1125	arg1	water					1150:1154	sea water	1146:1154	sea water	1146:1154	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	0	47	with	polymer	24:30	arg1	cavity					48:53	compatible cavity	37:53	compatible cavity	37:53	Magnetic γ-cyclodextrin polymer with compatible cavity promote the magnetic solid-phase extraction of microcystins in water samples.					
30712592	2	48	theme	excellent	420:428	arg1	properties					430:439	excellent properties	420:439	excellent properties for the magnetic solid-phase extraction (MSPE) of MCs	420:493	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	4	49	theme	good	821:824	arg1	R2 ≥ 0.9992					837:847	R2 ≥ 0.9992	837:847	R2 ≥ 0.9992	837:847	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	49	theme	good	821:824	arg1	linearity					826:834	good linearity	821:834	good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5)	821:1030	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	5	50	dep	extraction	1095:1104	arg1	the					1091:1093	the	1091:1093	the	1091:1093	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	2	51	theme	γ-cyclodextrin	332:345	arg1	polymer					347:353	the magnetic γ-cyclodextrin polymer	319:353	the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite	319:381	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	51	theme	γ-cyclodextrin	332:345	arg1	γ-CDP					366:370	Fe3O4@PDA@γ-CDP	356:370	Fe3O4@PDA@γ-CDP	356:370	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	5	52	theme	proposed	1046:1053	arg1	method					1055:1060	the proposed method	1042:1060	the proposed method	1042:1060	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	53	theme	MCs	1123:1125	arg1	extraction					1095:1104	extraction	1095:1104	extraction	1095:1104	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	53	theme	MCs	1123:1125	arg1	detection					1110:1118	detection	1110:1118	detection	1110:1118	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	3	54	theme	excellent	625:633	arg1	performance					646:656	an excellent extraction performance	622:656	an excellent extraction performance for trace MCs in water samples	622:687	The prepared Fe3O4@PDA@γ-CDP microspheres exhibit good aqueous dispersibility and highly affinity for MCs, thus contribute to an excellent extraction performance for trace MCs in water samples.					
30712592	2	55	with	adsorbent	405:413	arg1	properties					430:439	excellent properties	420:439	excellent properties for the magnetic solid-phase extraction (MSPE) of MCs	420:493	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	56	theme	magnetic	323:330	arg1	polymer					347:353	the magnetic γ-cyclodextrin polymer	319:353	the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite	319:381	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	56	theme	magnetic	323:330	arg1	γ-CDP					366:370	Fe3O4@PDA@γ-CDP	356:370	Fe3O4@PDA@γ-CDP	356:370	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	5	57	from	water	1150:1154	arg1	extraction					1095:1104	extraction	1095:1104	extraction	1095:1104	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	57	from	water	1150:1154	arg1	detection					1110:1118	detection	1110:1118	detection	1110:1118	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	58	from	water	1136:1140	arg1	extraction					1095:1104	extraction	1095:1104	extraction	1095:1104	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	58	from	water	1136:1140	arg1	detection					1110:1118	detection	1110:1118	detection	1110:1118	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	4	59	from	linearity	826:834	arg1	range					857:861	the range	853:861	the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5)	853:1030	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	60	theme	sensitive	725:733	arg1	method					749:754	a handy, sensitive and efficient method	716:754	a handy, sensitive and efficient method	716:754	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	61	theme	lower	1008:1012	arg1	RSDs					1002:1005	RSDs	1002:1005	RSDs	1002:1005	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	61	theme	lower	1008:1012	arg1	deviations					990:999	the relative standard deviations	968:999	the relative standard deviations (RSDs) lower than 6.2% (n = 5)	968:1030	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	0	62	theme	solid-phase	76:86	arg1	extraction					88:97	the magnetic solid-phase extraction	63:97	the magnetic solid-phase extraction of microcystins in water samples	63:130	Magnetic γ-cyclodextrin polymer with compatible cavity promote the magnetic solid-phase extraction of microcystins in water samples.					
30712592	5	63	theme	sea	1146:1148	arg1	water					1150:1154	sea water	1146:1154	sea water	1146:1154	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	5	64	theme	fresh	1130:1134	arg1	water					1136:1140	fresh water	1130:1140	fresh water	1130:1140	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	2	65	theme	PDA	362:364	arg1	polymer					347:353	the magnetic γ-cyclodextrin polymer	319:353	the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite	319:381	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	65	theme	PDA	362:364	arg1	γ-CDP					366:370	Fe3O4@PDA@γ-CDP	356:370	Fe3O4@PDA@γ-CDP	356:370	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	1	66	theme	humans	232:237	arg1	health					222:227	the health	218:227	the health of humans for their poisonousness	218:261	Microcystins (MCs), which are produced by eruptive cyanobacteria, seriously threaten the health of humans for their poisonousness.					
30712592	0	67	theme	magnetic	67:74	arg1	extraction					88:97	the magnetic solid-phase extraction	63:97	the magnetic solid-phase extraction of microcystins in water samples	63:130	Magnetic γ-cyclodextrin polymer with compatible cavity promote the magnetic solid-phase extraction of microcystins in water samples.					
30712592	4	68	theme	efficient	739:747	arg1	method					749:754	a handy, sensitive and efficient method	716:754	a handy, sensitive and efficient method	716:754	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	5	69	theme	sea	1241:1243	arg1	water					1245:1249	sea water	1241:1249	sea water	1241:1249	At last, the proposed method was successfully applied for the extraction and detection of MCs in fresh water and sea water samples, which provides great potential of trace-level MCs determination in lake and sea water.					
30712592	2	70	theme	@	361:361	arg1	polymer					347:353	the magnetic γ-cyclodextrin polymer	319:353	the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite	319:381	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	2	70	theme	@	361:361	arg1	γ-CDP					366:370	Fe3O4@PDA@γ-CDP	356:370	Fe3O4@PDA@γ-CDP	356:370	Herein, a facile strategy was introduced to synthesize the magnetic γ-cyclodextrin polymer (Fe3O4@PDA@γ-CDP) composite and set it as a novel adsorbent with excellent properties for the magnetic solid-phase extraction (MSPE) of MCs.					
30712592	4	71	theme	relative	972:979	arg1	RSDs					1002:1005	RSDs	1002:1005	RSDs	1002:1005	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30712592	4	71	theme	relative	972:979	arg1	deviations					990:999	the relative standard deviations	968:999	the relative standard deviations (RSDs) lower than 6.2% (n = 5)	968:1030	Combined with HPLC-MS/MS, a handy, sensitive and efficient method was developed for detection of MCs in water samples, which shows good linearity (R2 ≥ 0.9992) in the range of 1.0-1000 pg mL-1, low limits of detection (0.8-2.0 pg mL-1, S/N = 3), satisfactory repeatability with the relative standard deviations (RSDs) lower than 6.2% (n = 5).					
30794900	1	0	from	ions	391:394	arg1	water					399:403	water	399:403	water	399:403	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	5	1	theme	batch	973:977	arg1	scale					979:983	batch scale	973:983	batch scale	973:983	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	1	2	theme	technology	165:174	arg1	potential					139:147	The potential	135:147	The potential of hydrothermal technology	135:174	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	0	3	theme	bead	86:89	arg1	system					91:96	Aegle marmelos admixed chitosan bead system	54:96	Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention	54:132	Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.					
30794900	1	4	theme	excess	347:352	arg1	NO3-					363:366	NO3-	363:366	NO3-	363:366	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	1	4	theme	excess	347:352	arg1	nitrate					354:360	excess nitrate	347:360	excess nitrate (NO3-)	347:367	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	2	5	theme	@	533:533	arg1	CS					534:535	La2O3AM@CS	526:535	La2O3AM@CS	526:535	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	5	theme	@	533:533	arg1	marmelos					512:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos	443:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	6	theme	in	575:576	arg1	precipitation					583:595	in situ precipitation	575:595	in situ precipitation (In situ)	575:605	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	4	7	theme	BET	932:934	arg1	analysis					936:943	BET analysis	932:943	BET analysis	932:943	The characterization studies of the adsorbents such as FTIR, XRD, SEM and BET analysis were explored in detail.					
30794900	2	8	dep	in	575:576	arg1	situ					578:581	situ	578:581	situ	578:581	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	7	9	theme	La2O3AM	1331:1337	arg1	beads					1352:1356	La2O3AM@CS composite beads	1331:1356	La2O3AM@CS composite beads	1331:1356	The field trial investigations and reuse of La2O3AM@CS composite beads were also executed.					
30794900	2	10	theme	La2O3AM	526:532	arg1	CS					534:535	La2O3AM@CS	526:535	La2O3AM@CS	526:535	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	10	theme	La2O3AM	526:532	arg1	marmelos					512:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos	443:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	1	11	theme	methemoglobinemia	270:286	arg1	troubles					258:265	the troubles	254:265	the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water	254:403	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	2	12	theme	oxide	484:488	arg1	CS					534:535	La2O3AM@CS	526:535	La2O3AM@CS	526:535	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	12	theme	oxide	484:488	arg1	AM					521:522	AM	521:522	AM	521:522	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	12	theme	oxide	484:488	arg1	marmelos					512:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos	443:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	5	13	theme	adsorbate	1074:1082	arg1	strength					1084:1091	initial adsorbate strength	1066:1091	initial adsorbate strength	1066:1091	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	5	13	theme	adsorbate	1074:1082	arg1	time					1038:1041	contact time	1030:1041	contact time	1030:1041	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	7	14	theme	field	1291:1295	arg1	investigations					1303:1316	The field trial investigations	1287:1316	The field trial investigations	1287:1316	The field trial investigations and reuse of La2O3AM@CS composite beads were also executed.					
30794900	2	15	theme	PO43-	653:657	arg1	adsorption					659:668	PO43- adsorption	653:668	PO43- adsorption	653:668	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	5	16	theme	adsorbent	1048:1056	arg1	dosage					1058:1063	adsorbent dosage	1048:1063	adsorbent dosage	1048:1063	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	5	16	theme	adsorbent	1048:1056	arg1	time					1038:1041	contact time	1030:1041	contact time	1030:1041	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	0	17	theme	phosphate	114:122	arg1	retention					124:132	phosphate retention	114:132	phosphate retention	114:132	Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.					
30794900	3	18	theme	27.84	783:787	arg1	capacity					766:773	an enhanced nitrate and phosphate sorption capacity	723:773	capacity	766:773	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	3	18	theme	27.84	783:787	arg1	nitrate					735:741	an enhanced nitrate and phosphate sorption capacity	723:773	nitrate	735:741	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	3	18	theme	27.84	783:787	arg1	SC					776:777	SC	776:777	SC	776:777	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	6	19	theme	fitted	1146:1151	arg1	data					1166:1169	The fitted experimental data	1142:1169	The fitted experimental data of various isotherms and thermodynamic parameters	1142:1219	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	6	20	theme	PO43-	1262:1266	arg1	system					1279:1284	PO43- adsorption system	1262:1284	PO43- adsorption system	1262:1284	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	3	21	theme	composite	702:710	arg1	beads					712:716	La2O3AM@CS composite beads	691:716	La2O3AM@CS composite beads	691:716	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	2	22	theme	encapsulated	461:472	arg1	CS					534:535	La2O3AM@CS	526:535	La2O3AM@CS	526:535	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	22	theme	encapsulated	461:472	arg1	AM					521:522	AM	521:522	AM	521:522	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	22	theme	encapsulated	461:472	arg1	marmelos					512:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos	443:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	6	23	theme	thermodynamic	1196:1208	arg1	parameters					1210:1219	thermodynamic parameters	1196:1219	thermodynamic parameters	1196:1219	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	4	24	theme	adsorbents	894:903	arg1	studies					879:885	The characterization studies	858:885	The characterization studies of the adsorbents such as FTIR, XRD, SEM and BET analysis	858:943	The characterization studies of the adsorbents such as FTIR, XRD, SEM and BET analysis were explored in detail.					
30794900	2	25	theme	lanthanum	474:482	arg1	CS					534:535	La2O3AM@CS	526:535	La2O3AM@CS	526:535	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	25	theme	lanthanum	474:482	arg1	AM					521:522	AM	521:522	AM	521:522	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	25	theme	lanthanum	474:482	arg1	marmelos					512:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos	443:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	5	26	theme	competing	1094:1102	arg1	anions					1104:1109	competing anions	1094:1109	competing anions	1094:1109	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	5	26	theme	competing	1094:1102	arg1	time					1038:1041	contact time	1030:1041	contact time	1030:1041	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	2	27	theme	composite	538:546	arg1	beads					548:552	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	3	28	dep	in	842:843	arg1	situ					845:848	situ	845:848	situ	845:848	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	0	29	theme	Hydrothermal	0:11	arg1	encapsulation					13:25	Hydrothermal encapsulation	0:25	Hydrothermal encapsulation of lanthanum oxide	0:44	Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.					
30794900	1	30	theme	blue	289:292	arg1	syndrome					299:306	blue baby syndrome	289:306	blue baby syndrome	289:306	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	1	30	theme	blue	289:292	arg1	methemoglobinemia					270:286	methemoglobinemia	270:286	methemoglobinemia (blue baby syndrome)	270:307	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	7	31	theme	CS	1339:1340	arg1	beads					1352:1356	La2O3AM@CS composite beads	1331:1356	La2O3AM@CS composite beads	1331:1356	The field trial investigations and reuse of La2O3AM@CS composite beads were also executed.					
30794900	6	32	theme	various	1174:1180	arg1	isotherms					1182:1190	various isotherms	1174:1190	various isotherms	1174:1190	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	6	33	theme	parameters	1210:1219	arg1	data					1166:1169	The fitted experimental data	1142:1169	The fitted experimental data of various isotherms and thermodynamic parameters	1142:1219	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	0	34	theme	lanthanum	30:38	arg1	oxide					40:44	lanthanum oxide	30:44	lanthanum oxide	30:44	Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.					
30794900	1	35	theme	baby	294:297	arg1	syndrome					299:306	blue baby syndrome	289:306	blue baby syndrome	289:306	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	1	35	theme	baby	294:297	arg1	methemoglobinemia					270:286	methemoglobinemia	270:286	methemoglobinemia (blue baby syndrome)	270:307	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	2	36	theme	Aegle	506:510	arg1	CS					534:535	La2O3AM@CS	526:535	La2O3AM@CS	526:535	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	36	theme	Aegle	506:510	arg1	AM					521:522	AM	521:522	AM	521:522	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	36	theme	Aegle	506:510	arg1	marmelos					512:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos	443:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	3	37	theme	other	813:817	arg1	adsorbents					819:828	the other adsorbents	809:828	the other adsorbents prepared by in situ method	809:855	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	6	38	theme	system	1279:1284	arg1	nature					1243:1248	the feasible nature	1230:1248	the feasible nature of NO3- and PO43- adsorption system	1230:1284	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	5	39	theme	contact	1030:1036	arg1	strength					1084:1091	initial adsorbate strength	1066:1091	initial adsorbate strength	1066:1091	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	5	39	theme	contact	1030:1036	arg1	anions					1104:1109	competing anions	1094:1109	competing anions	1094:1109	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	5	39	theme	contact	1030:1036	arg1	dosage					1058:1063	adsorbent dosage	1048:1063	adsorbent dosage	1048:1063	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	5	39	theme	contact	1030:1036	arg1	time					1038:1041	contact time	1030:1041	contact time	1030:1041	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	5	39	theme	contact	1030:1036	arg1	temperature					1115:1125	temperature	1115:1125	temperature	1115:1125	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	5	39	theme	contact	1030:1036	arg1	pH					1044:1045	pH	1044:1045	pH	1044:1045	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	2	40	theme	admixed	498:504	arg1	CS					534:535	La2O3AM@CS	526:535	La2O3AM@CS	526:535	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	40	theme	admixed	498:504	arg1	AM					521:522	AM	521:522	AM	521:522	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	40	theme	admixed	498:504	arg1	marmelos					512:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos	443:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	1	41	theme	phosphate	373:381	arg1	ions					391:394	phosphate (PO43-) ions	373:394	phosphate (PO43-) ions in water	373:403	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	3	42	theme	34.91 mg/g	793:802	arg1	capacity					766:773	an enhanced nitrate and phosphate sorption capacity	723:773	capacity	766:773	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	3	42	theme	34.91 mg/g	793:802	arg1	nitrate					735:741	an enhanced nitrate and phosphate sorption capacity	723:773	nitrate	735:741	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	3	42	theme	34.91 mg/g	793:802	arg1	SC					776:777	SC	776:777	SC	776:777	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	1	43	theme	promising	212:220	arg1	adsorbents					222:231	promising adsorbents	212:231	promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water	212:403	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	6	44	theme	adsorption	1268:1277	arg1	system					1279:1284	PO43- adsorption system	1262:1284	PO43- adsorption system	1262:1284	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	7	45	theme	trial	1297:1301	arg1	investigations					1303:1316	The field trial investigations	1287:1316	The field trial investigations	1287:1316	The field trial investigations and reuse of La2O3AM@CS composite beads were also executed.					
30794900	3	46	theme	sorption	757:764	arg1	capacity					766:773	an enhanced nitrate and phosphate sorption capacity	723:773	capacity	766:773	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	1	47	theme	adsorbents	222:231	arg1	preparation					197:207	the preparation	193:207	the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water	193:403	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	0	48	theme	oxide	40:44	arg1	encapsulation					13:25	Hydrothermal encapsulation	0:25	Hydrothermal encapsulation of lanthanum oxide	0:44	Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.					
30794900	1	49	from	nitrate	354:360	arg1	water					399:403	water	399:403	water	399:403	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	1	50	theme	PO43-	384:388	arg1	ions					391:394	phosphate (PO43-) ions	373:394	phosphate (PO43-) ions in water	373:403	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	2	51	theme	La2O3	491:495	arg1	CS					534:535	La2O3AM@CS	526:535	La2O3AM@CS	526:535	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	51	theme	La2O3	491:495	arg1	AM					521:522	AM	521:522	AM	521:522	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	51	theme	La2O3	491:495	arg1	marmelos					512:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos	443:519	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	0	52	theme	marmelos	60:67	arg1	system					91:96	Aegle marmelos admixed chitosan bead system	54:96	Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention	54:132	Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.					
30794900	1	53	theme	eutrophication	313:326	arg1	troubles					258:265	the troubles	254:265	the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water	254:403	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	6	54	theme	experimental	1153:1164	arg1	data					1166:1169	The fitted experimental data	1142:1169	The fitted experimental data of various isotherms and thermodynamic parameters	1142:1219	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	3	55	theme	in	842:843	arg1	method					850:855	in situ method	842:855	in situ method	842:855	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	0	56	theme	Aegle	54:58	arg1	system					91:96	Aegle marmelos admixed chitosan bead system	54:96	Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention	54:132	Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.					
30794900	6	57	theme	isotherms	1182:1190	arg1	data					1166:1169	The fitted experimental data	1142:1169	The fitted experimental data of various isotherms and thermodynamic parameters	1142:1219	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	2	58	theme	present	420:426	arg1	investigation					428:440	the present investigation	416:440	the present investigation	416:440	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	2	59	dep	precipitation	583:595	arg1	situ					601:604	In situ	598:604	In situ	598:604	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	6	60	theme	feasible	1234:1241	arg1	nature					1243:1248	the feasible nature	1230:1248	the feasible nature of NO3- and PO43- adsorption system	1230:1284	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	4	61	theme	characterization	862:877	arg1	studies					879:885	The characterization studies	858:885	The characterization studies of the adsorbents such as FTIR, XRD, SEM and BET analysis	858:943	The characterization studies of the adsorbents such as FTIR, XRD, SEM and BET analysis were explored in detail.					
30794900	3	62	theme	phosphate	747:755	arg1	capacity					766:773	an enhanced nitrate and phosphate sorption capacity	723:773	capacity	766:773	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	3	63	theme	@	698:698	arg1	beads					712:716	La2O3AM@CS composite beads	691:716	La2O3AM@CS composite beads	691:716	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	2	64	theme	marmelos	512:519	arg1	beads					548:552	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads	443:552	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	7	65	theme	composite	1342:1350	arg1	beads					1352:1356	La2O3AM@CS composite beads	1331:1356	La2O3AM@CS composite beads	1331:1356	The field trial investigations and reuse of La2O3AM@CS composite beads were also executed.					
30794900	2	66	theme	hydrothermal	611:622	arg1	methods					632:638	hydrothermal (Hydro) methods	611:638	hydrothermal (Hydro) methods	611:638	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	3	67	theme	enhanced	726:733	arg1	nitrate					735:741	an enhanced nitrate and phosphate sorption capacity	723:773	nitrate	735:741	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	3	67	theme	enhanced	726:733	arg1	SC					776:777	SC	776:777	SC	776:777	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	3	68	theme	CS	699:700	arg1	beads					712:716	La2O3AM@CS composite beads	691:716	La2O3AM@CS composite beads	691:716	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
30794900	0	69	theme	chitosan	77:84	arg1	system					91:96	Aegle marmelos admixed chitosan bead system	54:96	Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention	54:132	Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.					
30794900	7	70	theme	beads	1352:1356	arg1	reuse					1322:1326	reuse	1322:1326	reuse of La2O3AM@CS composite beads	1322:1356	The field trial investigations and reuse of La2O3AM@CS composite beads were also executed.					
30794900	7	70	theme	beads	1352:1356	arg1	investigations					1303:1316	The field trial investigations	1287:1316	The field trial investigations	1287:1316	The field trial investigations and reuse of La2O3AM@CS composite beads were also executed.					
30794900	6	71	theme	NO3-	1253:1256	arg1	nature					1243:1248	the feasible nature	1230:1248	the feasible nature of NO3- and PO43- adsorption system	1230:1284	The fitted experimental data of various isotherms and thermodynamic parameters supports the feasible nature of NO3- and PO43- adsorption system.					
30794900	5	72	theme	initial	1066:1072	arg1	strength					1084:1091	initial adsorbate strength	1066:1091	initial adsorbate strength	1066:1091	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	5	72	theme	initial	1066:1072	arg1	time					1038:1041	contact time	1030:1041	contact time	1030:1041	In batch scale, the adsorption affecting parameters such as contact time, pH, adsorbent dosage, initial adsorbate strength, competing anions and temperature was optimized.					
30794900	0	73	theme	admixed	69:75	arg1	system					91:96	Aegle marmelos admixed chitosan bead system	54:96	Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention	54:132	Hydrothermal encapsulation of lanthanum oxide derived Aegle marmelos admixed chitosan bead system for nitrate and phosphate retention.					
30794900	7	74	theme	@	1338:1338	arg1	beads					1352:1356	La2O3AM@CS composite beads	1331:1356	La2O3AM@CS composite beads	1331:1356	The field trial investigations and reuse of La2O3AM@CS composite beads were also executed.					
30794900	1	75	used	utilized	180:187	arg2	potential					139:147	The potential	135:147	The potential of hydrothermal technology	135:174	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	2	76	theme	Hydro	625:629	arg1	methods					632:638	hydrothermal (Hydro) methods	611:638	hydrothermal (Hydro) methods	611:638	Hence, in the present investigation, the chitosan (CS) encapsulated lanthanum oxide (La2O3) admixed Aegle marmelos(AM) (La2O3AM@CS) composite beads was prepared by both in situ precipitation (In situ) and hydrothermal (Hydro) methods for NO3- and PO43- adsorption.					
30794900	1	77	theme	hydrothermal	152:163	arg1	technology					165:174	hydrothermal technology	152:174	hydrothermal technology	152:174	The potential of hydrothermal technology was utilized for the preparation of promising adsorbents in order to overcome the troubles of methemoglobinemia (blue baby syndrome) and eutrophication which is caused by excess nitrate (NO3-) and phosphate (PO43-) ions in water.					
30794900	3	78	theme	La2O3AM	691:697	arg1	beads					712:716	La2O3AM@CS composite beads	691:716	La2O3AM@CS composite beads	691:716	The hydro supported La2O3AM@CS composite beads hold an enhanced nitrate and phosphate sorption capacity (SC) of 27.84 and 34.91 mg/g than the other adsorbents prepared by in situ method.					
29860339	12	0	dep	greater	2209:2215	arg1	<					2220:2220	P < 0.001	2218:2226	P < 0.001	2218:2226	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	3	1	theme	40	849:850	arg1	%					851:851	%	851:851	%	851:851	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	14	2	theme	serum	2563:2567	arg1	biomarkers					2569:2578	piglet serum biomarkers	2556:2578	piglet serum biomarkers	2556:2578	In conclusion, nutritional management of the developing gilt may affect milk nutrient composition, milk OS profile, and piglet serum biomarkers.					
29860339	2	3	theme	progeny	588:594	arg1	biomarkers					596:605	postnatal progeny biomarkers	578:605	postnatal progeny biomarkers	578:605	Therefore, we hypothesized that energy restriction during gilt development may affect milk nutrient profile, milk oligosaccharides (OS), and postnatal progeny biomarkers.					
29860339	7	4	dep	gilts	1443:1447	arg1	n					1450:1450	n = 7	1450:1454	n = 7	1450:1454	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	3	5	theme	=	657:657	arg1	n					655:655	n = 128	655:661	n = 128	655:661	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	5	theme	=	657:657	arg1	gilts					648:652	batch 14 gilts	639:652	batch 14 gilts (n = 128, 8 gilts/pen)	639:675	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	5	6	theme	daily	1152:1156	arg1	Mcal/d					1187:1192	Mcal/d	1187:1192	Mcal/d	1187:1192	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	5	6	theme	daily	1152:1156	arg1	intake					1179:1184	daily metabolizable energy intake	1152:1184	daily metabolizable energy intake (Mcal/d)	1152:1193	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	8	7	theme	peptide-2	1700:1708	arg1	quantification					1668:1681	quantification	1668:1681	quantification of glucagon-like peptide-2 (GLP-2) and insulin	1668:1728	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	11	8	from	OS	2010:2011	arg1	day					2027:2029	day 0	2027:2031	day 0	2027:2031	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	5	9	theme	energy	1172:1177	arg1	Mcal/d					1187:1192	Mcal/d	1187:1192	Mcal/d	1187:1192	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	5	9	theme	energy	1172:1177	arg1	intake					1179:1184	daily metabolizable energy intake	1152:1184	daily metabolizable energy intake (Mcal/d)	1152:1193	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	8	10	dep	samples	1590:1596	arg1	n					1599:1599	n	1599:1599	n = 6 piglets/gilt	1599:1616	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	7	11	theme	matter	1543:1548	arg1	analysis					1520:1527	compositional analysis	1506:1527	compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS	1506:1574	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	2	12	theme	milk	523:526	arg1	profile					537:543	milk nutrient profile	523:543	milk nutrient profile	523:543	Therefore, we hypothesized that energy restriction during gilt development may affect milk nutrient profile, milk oligosaccharides (OS), and postnatal progeny biomarkers.					
29860339	9	13	theme	P	1853:1853	arg1	<					1855:1855	P < 0.05	1853:1860	P < 0.05	1853:1860	No effects of developmental diet were observed for milk N, CP, DM, or GE; however, N, CP, DM, and insulin were increased (P < 0.05) on day 1 compared with day 14.					
29860339	0	14	theme	progeny	107:113	arg1	biomarkers					115:124	progeny biomarkers	107:124	progeny biomarkers	107:124	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	4	15	dep	days	1080:1083	arg1	to					1089:1090	to	1089:1090	to	1089:1090	All diets were fed ad libitum and applied in a 3-phase feeding regimen during gilt development (days 123 to 230 of age).					
29860339	14	16	theme	OS	2540:2541	arg1	profile					2543:2549	milk OS profile	2535:2549	milk OS profile	2535:2549	In conclusion, nutritional management of the developing gilt may affect milk nutrient composition, milk OS profile, and piglet serum biomarkers.					
29860339	13	17	theme	P	2407:2407	arg1	<					2409:2409	P < 0.03	2407:2414	P < 0.03	2407:2414	For serum insulin, a treatment by day interaction was observed (P < 0.01); specifically, insulin in RESTR progeny was greater (P < 0.03) than CTL on day 1.					
29860339	13	18	theme	RESTR	2380:2384	arg1	progeny					2386:2392	RESTR progeny	2380:2392	RESTR progeny	2380:2392	For serum insulin, a treatment by day interaction was observed (P < 0.01); specifically, insulin in RESTR progeny was greater (P < 0.03) than CTL on day 1.					
29860339	9	19	theme	developmental	1745:1757	arg1	diet					1759:1762	developmental diet	1745:1762	developmental diet	1745:1762	No effects of developmental diet were observed for milk N, CP, DM, or GE; however, N, CP, DM, and insulin were increased (P < 0.05) on day 1 compared with day 14.					
29860339	2	20	theme	milk	546:549	arg1	OS					569:570	OS	569:570	OS	569:570	Therefore, we hypothesized that energy restriction during gilt development may affect milk nutrient profile, milk oligosaccharides (OS), and postnatal progeny biomarkers.					
29860339	2	20	theme	milk	546:549	arg1	oligosaccharides					551:566	milk oligosaccharides	546:566	milk oligosaccharides (OS)	546:571	Therefore, we hypothesized that energy restriction during gilt development may affect milk nutrient profile, milk oligosaccharides (OS), and postnatal progeny biomarkers.					
29860339	10	21	theme	different	1908:1916	arg1	OS					1923:1924	61 different milk OS	1905:1924	61 different milk OS	1905:1924	A total of 61 different milk OS were identified.					
29860339	1	22	theme	progeny	378:384	arg1	health					386:391	progeny health	378:391	progeny health	378:391	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	1	22	theme	progeny	378:384	arg1	progeny					428:434	parity 1 progeny	419:434	parity 1 progeny	419:434	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	8	23	dep	days	1636:1639	arg1	1					1641:1641	1	1641:1641	1	1641:1641	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	8	23	dep	days	1636:1639	arg1	15					1647:1648	15	1647:1648	15	1647:1648	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	13	24	theme	serum	2284:2288	arg1	insulin					2290:2296	serum insulin	2284:2296	serum insulin	2284:2296	For serum insulin, a treatment by day interaction was observed (P < 0.01); specifically, insulin in RESTR progeny was greater (P < 0.03) than CTL on day 1.					
29860339	14	25	theme	nutrient	2513:2520	arg1	composition					2522:2532	milk nutrient composition	2508:2532	milk nutrient composition	2508:2532	In conclusion, nutritional management of the developing gilt may affect milk nutrient composition, milk OS profile, and piglet serum biomarkers.					
29860339	13	26	dep	greater	2398:2404	arg1	<					2409:2409	P < 0.03	2407:2414	P < 0.03	2407:2414	For serum insulin, a treatment by day interaction was observed (P < 0.01); specifically, insulin in RESTR progeny was greater (P < 0.03) than CTL on day 1.					
29860339	0	27	theme	milk	57:60	arg1	profile					71:77	milk nutrient profile	57:77	milk nutrient profile	57:77	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	12	28	theme	day	2120:2122	arg1	interaction					2124:2134	day interaction	2120:2134	day interaction	2120:2134	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	1	29	from	University	151:160	arg1	study					138:142	An ongoing study	127:142	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch)	127:237	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	14	30	theme	gilt	2492:2495	arg1	management					2463:2472	nutritional management	2451:2472	nutritional management of the developing gilt	2451:2495	In conclusion, nutritional management of the developing gilt may affect milk nutrient composition, milk OS profile, and piglet serum biomarkers.					
29860339	10	31	theme	OS	1923:1924	arg1	total					1896:1900	A total	1894:1900	A total of 61 different milk OS	1894:1924	A total of 61 different milk OS were identified.					
29860339	1	32	theme	gilt	313:316	arg1	period					301:306	the developmental period	283:306	the developmental period of a gilt	283:316	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	7	33	theme	N	1532:1532	arg1	analysis					1520:1527	compositional analysis	1506:1527	compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS	1506:1574	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	4	34	dep	development	1067:1077	arg1	days					1080:1083	days 123 to 230	1080:1094	days 123 to 230 of age	1080:1101	All diets were fed ad libitum and applied in a 3-phase feeding regimen during gilt development (days 123 to 230 of age).					
29860339	2	35	theme	gilt	495:498	arg1	development					500:510	gilt development	495:510	gilt development	495:510	Therefore, we hypothesized that energy restriction during gilt development may affect milk nutrient profile, milk oligosaccharides (OS), and postnatal progeny biomarkers.					
29860339	0	36	from	Effects	0:6	arg1	profile					71:77	milk nutrient profile	57:77	milk nutrient profile	57:77	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	0	36	from	Effects	0:6	arg1	oligosaccharides					85:100	milk oligosaccharides	80:100	milk oligosaccharides	80:100	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	0	36	from	Effects	0:6	arg1	biomarkers					115:124	progeny biomarkers	107:124	progeny biomarkers	107:124	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	0	37	theme	milk	80:83	arg1	oligosaccharides					85:100	milk oligosaccharides	80:100	milk oligosaccharides	80:100	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	12	38	theme	P	2150:2150	arg1	<					2152:2152	P < 0.009	2150:2158	P < 0.009	2150:2158	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	9	39	theme	diet	1759:1762	arg1	effects					1734:1740	No effects	1731:1740	No effects of developmental diet	1731:1762	No effects of developmental diet were observed for milk N, CP, DM, or GE; however, N, CP, DM, and insulin were increased (P < 0.05) on day 1 compared with day 14.					
29860339	3	40	theme	acids	923:927	arg1	addition					893:900	addition	893:900	addition of crystalline amino acids equivalent to the SID Lys	893:953	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	40	theme	acids	923:927	arg1	diet					883:886	3) CTL diet	876:886	3) CTL diet	876:886	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	8	41	theme	Piglet	1577:1582	arg1	samples					1590:1596	Piglet blood samples	1577:1596	Piglet blood samples (n = 6 piglets/gilt)	1577:1617	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	6	42	theme	230	1348:1350	arg1	d					1352:1352	230 d	1348:1352	230 d of age	1348:1359	After 230 d of age, gilts were bred and fed a common diet.					
29860339	3	43	dep	diet	883:886	arg1	3					876:876	3	876:876	3	876:876	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	44	theme	crystalline	905:915	arg1	acids					923:927	crystalline amino acids	905:927	crystalline amino acids equivalent to the SID Lys	905:953	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	45	theme	20	810:811	arg1	%					812:812	%	812:812	%	812:812	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	9	46	dep	increased	1842:1850	arg1	<					1855:1855	P < 0.05	1853:1860	P < 0.05	1853:1860	No effects of developmental diet were observed for milk N, CP, DM, or GE; however, N, CP, DM, and insulin were increased (P < 0.05) on day 1 compared with day 14.					
29860339	5	47	theme	Phase	1242:1246	arg1	Phase					1214:1218	Phase 1: 10.13, 6.97, 9.95	1214:1239	Phase	1214:1218	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	5	47	theme	Phase	1242:1246	arg1	phase					1207:1211	each phase	1202:1211	each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07)	1202:1300	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	9	48	theme	milk	1782:1785	arg1	N					1787:1787	milk N	1782:1787	milk N	1782:1787	No effects of developmental diet were observed for milk N, CP, DM, or GE; however, N, CP, DM, and insulin were increased (P < 0.05) on day 1 compared with day 14.					
29860339	0	49	theme	energy	11:16	arg1	restriction					18:28	energy restriction	11:28	energy restriction	11:28	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	1	50	theme	energy	257:262	arg1	restriction					264:274	energy restriction	257:274	energy restriction during the developmental period of a gilt	257:316	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	3	51	theme	SID	947:949	arg1	Lys					951:953	the SID Lys	943:953	the SID Lys	943:953	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	52	theme	Restricted	798:807	arg1	diet					743:746	1) Control diet	732:746	the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+)	717:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	52	theme	Restricted	798:807	arg1	restriction					821:831	20% energy restriction	810:831	20% energy restriction via addition of 40% soy hulls	810:861	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	12	53	theme	GLP	2185:2187	arg1	concentrations					2189:2202	day 1 GLP concentrations	2179:2202	day 1 GLP concentrations	2179:2202	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	1	54	theme	developmental	287:299	arg1	period					301:306	the developmental period	283:306	the developmental period of a gilt	283:316	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	3	55	theme	energy	814:819	arg1	diet					743:746	1) Control diet	732:746	the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+)	717:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	55	theme	energy	814:819	arg1	restriction					821:831	20% energy restriction	810:831	20% energy restriction via addition of 40% soy hulls	810:861	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	11	56	theme	P	2014:2014	arg1	OS					2010:2011	neutral and acidic OS	1991:2011	neutral and acidic OS (P < 0.05) on day 0	1991:2031	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	11	56	theme	P	2014:2014	arg1	<					2016:2016	P < 0.05	2014:2021	P < 0.05	2014:2021	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	3	57	theme	batch	639:643	arg1	n					655:655	n = 128	655:661	n = 128	655:661	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	57	theme	batch	639:643	arg1	gilts					648:652	batch 14 gilts	639:652	batch 14 gilts (n = 128, 8 gilts/pen)	639:675	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	58	theme	%	851:851	arg1	hulls					857:861	40% soy hulls	849:861	40% soy hulls	849:861	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	4	59	theme	feeding	1039:1045	arg1	regimen					1047:1053	a 3-phase feeding regimen	1029:1053	a 3-phase feeding regimen	1029:1053	All diets were fed ad libitum and applied in a 3-phase feeding regimen during gilt development (days 123 to 230 of age).					
29860339	5	60	theme	daily	1113:1117	arg1	intake					1124:1129	Average daily feed intake	1105:1129	Average daily feed intake	1105:1129	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	13	61	from	CTL	2422:2424	arg1	day					2429:2431	day 1	2429:2433	day 1	2429:2433	For serum insulin, a treatment by day interaction was observed (P < 0.01); specifically, insulin in RESTR progeny was greater (P < 0.03) than CTL on day 1.					
29860339	13	62	theme	day	2314:2316	arg1	interaction					2318:2328	day interaction	2314:2328	day interaction	2314:2328	For serum insulin, a treatment by day interaction was observed (P < 0.01); specifically, insulin in RESTR progeny was greater (P < 0.03) than CTL on day 1.					
29860339	11	63	theme	Milk	1943:1946	arg1	profile					1951:1957	Milk OS profile	1943:1957	Milk OS profile	1943:1957	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	5	64	theme	Phase	1275:1279	arg1	Phase					1214:1218	Phase 1: 10.13, 6.97, 9.95	1214:1239	Phase	1214:1218	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	5	64	theme	Phase	1275:1279	arg1	phase					1207:1211	each phase	1202:1211	each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07)	1202:1300	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	12	65	theme	day	2179:2181	arg1	concentrations					2189:2202	day 1 GLP concentrations	2179:2202	day 1 GLP concentrations	2179:2202	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	3	66	theme	RESTR	965:969	arg1	diet					971:974	the RESTR diet	961:974	the RESTR diet (CTL+)	961:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	66	theme	RESTR	965:969	arg1	CTL+					977:980	CTL+	977:980	CTL+	977:980	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	7	67	theme	Milk	1401:1404	arg1	samples					1406:1412	Milk samples	1401:1412	Milk samples	1401:1412	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	5	68	used	used	1135:1138	arg2	intake					1124:1129	Average daily feed intake	1105:1129	Average daily feed intake	1105:1129	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	14	69	theme	piglet	2556:2561	arg1	biomarkers					2569:2578	piglet serum biomarkers	2556:2578	piglet serum biomarkers	2556:2578	In conclusion, nutritional management of the developing gilt may affect milk nutrient composition, milk OS profile, and piglet serum biomarkers.					
29860339	3	70	dep	following	721:729	arg1	ME					955:956	ME	955:956	the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+)	717:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	70	dep	following	721:729	arg1	addition					893:900	addition	893:900	addition of crystalline amino acids equivalent to the SID Lys	893:953	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	70	dep	following	721:729	arg1	diet					743:746	1) Control diet	732:746	the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+)	717:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	70	dep	following	721:729	arg1	diet					883:886	3) CTL diet	876:886	3) CTL diet	876:886	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	70	dep	following	721:729	arg1	restriction					821:831	20% energy restriction	810:831	20% energy restriction via addition of 40% soy hulls	810:861	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	7	71	theme	insulin	1560:1566	arg1	analysis					1520:1527	compositional analysis	1506:1527	compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS	1506:1574	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	3	72	theme	hulls	857:861	arg1	addition					837:844	addition	837:844	addition of 40% soy hulls	837:861	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	1	73	theme	parity	419:424	arg1	progeny					428:434	parity 1 progeny	419:434	parity 1 progeny	419:434	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	1	73	theme	parity	419:424	arg1	health					386:391	progeny health	378:391	progeny health	378:391	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	8	74	dep	=	1601:1601	arg1	piglets/gilt					1605:1616	6 piglets/gilt	1603:1616	6 piglets/gilt	1603:1616	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	3	75	dep	restriction	821:831	arg1	RESTR					864:868	RESTR	864:868	RESTR	864:868	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	7	76	theme	GE	1556:1557	arg1	analysis					1520:1527	compositional analysis	1506:1527	compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS	1506:1574	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	2	77	theme	postnatal	578:586	arg1	biomarkers					596:605	postnatal progeny biomarkers	578:605	postnatal progeny biomarkers	578:605	Therefore, we hypothesized that energy restriction during gilt development may affect milk nutrient profile, milk oligosaccharides (OS), and postnatal progeny biomarkers.					
29860339	5	78	theme	metabolizable	1158:1170	arg1	Mcal/d					1187:1192	Mcal/d	1187:1192	Mcal/d	1187:1192	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	5	78	theme	metabolizable	1158:1170	arg1	intake					1179:1184	daily metabolizable energy intake	1152:1184	daily metabolizable energy intake (Mcal/d)	1152:1193	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	8	79	theme	glucagon-like	1686:1698	arg1	peptide-2					1700:1708	glucagon-like peptide-2	1686:1708	glucagon-like peptide-2 (GLP-2)	1686:1716	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	8	79	theme	glucagon-like	1686:1698	arg1	GLP-2					1711:1715	GLP-2	1711:1715	GLP-2	1711:1715	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	11	80	theme	significant	2052:2062	arg1	differences					2064:2074	no significant differences	2049:2074	no significant differences	2049:2074	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	12	81	dep	observed	2140:2147	arg1	on					2176:2177	on	2176:2177	on	2176:2177	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	12	81	dep	observed	2140:2147	arg1	greater					2209:2215	greater	2209:2215	greater	2209:2215	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	12	81	dep	observed	2140:2147	arg1	<					2152:2152	P < 0.009	2150:2158	P < 0.009	2150:2158	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	1	82	theme	beneficial	356:365	arg1	effects					367:373	beneficial effects	356:373	beneficial effects	356:373	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	7	83	theme	dry	1539:1541	arg1	DM					1551:1552	DM	1551:1552	DM	1551:1552	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	7	83	theme	dry	1539:1541	arg1	matter					1543:1548	dry matter	1539:1548	dry matter (DM)	1539:1553	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	14	84	theme	milk	2535:2538	arg1	profile					2543:2549	milk OS profile	2535:2549	milk OS profile	2535:2549	In conclusion, nutritional management of the developing gilt may affect milk nutrient composition, milk OS profile, and piglet serum biomarkers.					
29860339	7	85	theme	CP	1535:1536	arg1	analysis					1520:1527	compositional analysis	1506:1527	compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS	1506:1574	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	8	86	theme	insulin	1722:1728	arg1	quantification					1668:1681	quantification	1668:1681	quantification of glucagon-like peptide-2 (GLP-2) and insulin	1668:1728	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	7	87	theme	compositional	1506:1518	arg1	analysis					1520:1527	compositional analysis	1506:1527	compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS	1506:1574	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	3	88	theme	dietary	688:694	arg1	treatments					696:705	3 dietary treatments	686:705	3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+)	686:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	88	theme	dietary	688:694	arg1	following					721:729	the following	717:729	the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+)	717:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	89	theme	2012	767:770	arg1	specifications					773:786	NRC (2012) specifications	762:786	NRC (2012) specifications (CTL)	762:792	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	89	theme	2012	767:770	arg1	CTL					789:791	CTL	789:791	CTL	789:791	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	2	90	theme	nutrient	528:535	arg1	profile					537:543	milk nutrient profile	523:543	milk nutrient profile	523:543	Therefore, we hypothesized that energy restriction during gilt development may affect milk nutrient profile, milk oligosaccharides (OS), and postnatal progeny biomarkers.					
29860339	14	91	theme	milk	2508:2511	arg1	composition					2522:2532	milk nutrient composition	2508:2532	milk nutrient composition	2508:2532	In conclusion, nutritional management of the developing gilt may affect milk nutrient composition, milk OS profile, and piglet serum biomarkers.					
29860339	0	92	theme	nutrient	62:69	arg1	profile					71:77	milk nutrient profile	57:77	milk nutrient profile	57:77	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	1	93	theme	ongoing	130:136	arg1	study					138:142	An ongoing study	127:142	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch)	127:237	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	3	94	theme	NRC	762:764	arg1	specifications					773:786	NRC (2012) specifications	762:786	NRC (2012) specifications (CTL)	762:792	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	94	theme	NRC	762:764	arg1	CTL					789:791	CTL	789:791	CTL	789:791	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	13	95	dep	observed	2334:2341	arg1	<					2346:2346	P < 0.01	2344:2351	P < 0.01	2344:2351	For serum insulin, a treatment by day interaction was observed (P < 0.01); specifically, insulin in RESTR progeny was greater (P < 0.03) than CTL on day 1.					
29860339	13	96	from	insulin	2369:2375	arg1	progeny					2386:2392	RESTR progeny	2380:2392	RESTR progeny	2380:2392	For serum insulin, a treatment by day interaction was observed (P < 0.01); specifically, insulin in RESTR progeny was greater (P < 0.03) than CTL on day 1.					
29860339	14	97	theme	developing	2481:2490	arg1	gilt					2492:2495	the developing gilt	2477:2495	the developing gilt	2477:2495	In conclusion, nutritional management of the developing gilt may affect milk nutrient composition, milk OS profile, and piglet serum biomarkers.					
29860339	10	98	theme	milk	1918:1921	arg1	OS					1923:1924	61 different milk OS	1905:1924	61 different milk OS	1905:1924	A total of 61 different milk OS were identified.					
29860339	3	99	theme	CTL	879:881	arg1	diet					883:886	3) CTL diet	876:886	3) CTL diet	876:886	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	6	100	theme	age	1357:1359	arg1	d					1352:1352	230 d	1348:1352	230 d of age	1348:1359	After 230 d of age, gilts were bred and fed a common diet.					
29860339	3	101	theme	Control	735:741	arg1	diet					743:746	1) Control diet	732:746	the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+)	717:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	101	theme	Control	735:741	arg1	restriction					821:831	20% energy restriction	810:831	20% energy restriction via addition of 40% soy hulls	810:861	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	102	theme	amino	917:921	arg1	acids					923:927	crystalline amino acids	905:927	crystalline amino acids equivalent to the SID Lys	905:953	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	8	103	theme	blood	1584:1588	arg1	samples					1590:1596	Piglet blood samples	1577:1596	Piglet blood samples (n = 6 piglets/gilt)	1577:1617	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	2	104	theme	energy	469:474	arg1	restriction					476:486	energy restriction	469:486	energy restriction during gilt development	469:510	Therefore, we hypothesized that energy restriction during gilt development may affect milk nutrient profile, milk oligosaccharides (OS), and postnatal progeny biomarkers.					
29860339	14	105	theme	nutritional	2451:2461	arg1	management					2463:2472	nutritional management	2451:2472	nutritional management of the developing gilt	2451:2495	In conclusion, nutritional management of the developing gilt may affect milk nutrient composition, milk OS profile, and piglet serum biomarkers.					
29860339	7	106	theme	OS	1573:1574	arg1	analysis					1520:1527	compositional analysis	1506:1527	compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS	1506:1574	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	11	107	theme	acidic	2003:2008	arg1	OS					2010:2011	neutral and acidic OS	1991:2011	neutral and acidic OS (P < 0.05) on day 0	1991:2031	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	11	107	theme	acidic	2003:2008	arg1	<					2016:2016	P < 0.05	2014:2021	P < 0.05	2014:2021	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	4	108	theme	ad	1003:1004	arg1	libitum					1006:1012	ad libitum	1003:1012	ad libitum	1003:1012	All diets were fed ad libitum and applied in a 3-phase feeding regimen during gilt development (days 123 to 230 of age).					
29860339	1	109	contain	have	351:354	arg1	restriction					264:274	energy restriction	257:274	energy restriction during the developmental period of a gilt	257:316	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	1	109	contain	have	351:354	arg2	effects					367:373	beneficial effects	356:373	beneficial effects	356:373	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	0	110	theme	restriction	18:28	arg1	Effects					0:6	Effects	0:6	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.	0:125	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	3	111	theme	%	812:812	arg1	diet					743:746	1) Control diet	732:746	the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+)	717:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	111	theme	%	812:812	arg1	restriction					821:831	20% energy restriction	810:831	20% energy restriction via addition of 40% soy hulls	810:861	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	8	112	theme	=	1601:1601	arg1	n					1599:1599	n	1599:1599	n = 6 piglets/gilt	1599:1616	Piglet blood samples (n = 6 piglets/gilt) were obtained on days 1 and 15 postfarrowing for quantification of glucagon-like peptide-2 (GLP-2) and insulin.					
29860339	3	113	theme	soy	853:855	arg1	hulls					857:861	40% soy hulls	849:861	40% soy hulls	849:861	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	11	114	theme	neutral	1991:1997	arg1	OS					2010:2011	neutral and acidic OS	1991:2011	neutral and acidic OS (P < 0.05) on day 0	1991:2031	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	11	114	theme	neutral	1991:1997	arg1	<					2016:2016	P < 0.05	2014:2021	P < 0.05	2014:2021	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	4	115	theme	gilt	1062:1065	arg1	development					1067:1077	gilt development	1062:1077	gilt development (days 123 to 230 of age)	1062:1102	All diets were fed ad libitum and applied in a 3-phase feeding regimen during gilt development (days 123 to 230 of age).					
29860339	0	116	theme	gilt	37:40	arg1	development					42:52	gilt development	37:52	gilt development	37:52	Effects of energy restriction during gilt development on milk nutrient profile, milk oligosaccharides, and progeny biomarkers.					
29860339	3	117	dep	n	655:655	arg1	gilts/pen					666:674	8 gilts/pen	664:674	8 gilts/pen	664:674	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	3	118	theme	equivalent	929:938	arg1	acids					923:927	crystalline amino acids	905:927	crystalline amino acids equivalent to the SID Lys	905:953	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	12	119	theme	piglet	2091:2096	arg1	GLP-2					2098:2102	piglet GLP-2	2091:2102	piglet GLP-2	2091:2102	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	6	120	theme	common	1388:1393	arg1	diet					1395:1398	a common diet	1386:1398	a common diet	1386:1398	After 230 d of age, gilts were bred and fed a common diet.					
29860339	5	121	theme	11.25	1251:1255	arg1	Phase					1214:1218	Phase 1: 10.13, 6.97, 9.95	1214:1239	Phase	1214:1218	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	5	121	theme	11.25	1251:1255	arg1	phase					1207:1211	each phase	1202:1211	each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07)	1202:1300	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	12	122	theme	P	2218:2218	arg1	<					2220:2220	P < 0.001	2218:2226	P < 0.001	2218:2226	For piglet GLP-2, a treatment by day interaction was observed (P < 0.009); specifically, on day 1 GLP concentrations were greater (P < 0.001) in CTL+ compared with RESTR (6.73 vs. 1.21 ng/mL).					
29860339	4	123	theme	3-phase	1031:1037	arg1	regimen					1047:1053	a 3-phase feeding regimen	1029:1053	a 3-phase feeding regimen	1029:1053	All diets were fed ad libitum and applied in a 3-phase feeding regimen during gilt development (days 123 to 230 of age).					
29860339	7	124	theme	=	1452:1452	arg1	n					1450:1450	n = 7	1450:1454	n = 7	1450:1454	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	13	125	theme	P	2344:2344	arg1	<					2346:2346	P < 0.01	2344:2351	P < 0.01	2344:2351	For serum insulin, a treatment by day interaction was observed (P < 0.01); specifically, insulin in RESTR progeny was greater (P < 0.03) than CTL on day 1.					
29860339	11	126	theme	OS	1948:1949	arg1	profile					1951:1957	Milk OS profile	1943:1957	Milk OS profile	1943:1957	Milk OS profile was significantly different for neutral and acidic OS (P < 0.05) on day 0, but there were no significant differences on day 14.					
29860339	3	127	theme	development	619:629	arg1	period					631:636	the development period	615:636	the development period	615:636	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	5	128	theme	Average	1105:1111	arg1	intake					1124:1129	Average daily feed intake	1105:1129	Average daily feed intake	1105:1129	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
29860339	1	129	theme	gilts	212:216	arg1	batches					201:207	14 batches	198:207	14 batches of gilts	198:216	An ongoing study at the University of Nebraska-Lincoln (which included 14 batches of gilts; n = 90 gilts/batch) demonstrated that energy restriction during the developmental period of a gilt increases longevity and may also have beneficial effects on progeny health and growth, particularly, parity 1 progeny.					
29860339	3	130	theme	diet	971:974	arg1	ME					955:956	ME	955:956	the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+)	717:981	During the development period, batch 14 gilts (n = 128, 8 gilts/pen) were fed 3 dietary treatments including the following: 1) Control diet formulated to NRC (2012) specifications (CTL); 2) Restricted (20% energy restriction via addition of 40% soy hulls; RESTR); and 3) CTL diet plus addition of crystalline amino acids equivalent to the SID Lys:ME of the RESTR diet (CTL+).					
29860339	7	131	theme	batch	1434:1438	arg1	gilts					1443:1447	batch 14 gilts	1434:1447	batch 14 gilts (n = 7 per treatment)	1434:1469	Milk samples were collected from batch 14 gilts (n = 7 per treatment) on days 0 and 14 postfarrowing for compositional analysis of N, CP, dry matter (DM), GE, insulin, and OS.					
29860339	4	132	theme	age	1099:1101	arg1	days					1080:1083	days 123 to 230	1080:1094	days 123 to 230 of age	1080:1101	All diets were fed ad libitum and applied in a 3-phase feeding regimen during gilt development (days 123 to 230 of age).					
29860339	5	133	theme	feed	1119:1122	arg1	intake					1124:1129	Average daily feed intake	1105:1129	Average daily feed intake	1105:1129	Average daily feed intake was used to estimate daily metabolizable energy intake (Mcal/d) during each phase (Phase 1: 10.13, 6.97, 9.95; Phase 2: 11.25, 8.05, 10.94; and Phase 3: 9.47, 7.95,11.07) for CTL, RESTR, and CTL+, respectively.					
31760013	0	0	theme	red	82:84	arg1	dye					90:92	reactive red 120 dye	73:92	reactive red 120 dye	73:92	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	2	1	theme	TiO2	320:323	arg1	nanoparticles					400:412	50% TiO2 nanoparticles	391:412	50% TiO2 nanoparticles (CS-GLA/TNC-50)	391:428	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	2	1	theme	TiO2	320:323	arg1	nanoparticles					325:337	TiO2 nanoparticles	320:337	TiO2 nanoparticles	320:337	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	2	1	theme	TiO2	320:323	arg1	nanoparticles					357:369	25% TiO2 nanoparticles	348:369	25% TiO2 nanoparticles (CS-GLA/TNC-25)	348:385	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	8	2	from	mechanism	1192:1200	arg1	surface					1232:1238	the CS-GLA/TNC-25 surface	1214:1238	the CS-GLA/TNC-25 surface	1214:1238	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	0	3	theme	reactive	73:80	arg1	dye					90:92	reactive red 120 dye	73:92	reactive red 120 dye	73:92	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	0	4	link	base-cross-linked	17:33	arg1	composite					44:52	Tunable Schiff's base-cross-linked chitosan composite	0:52	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.	0:125	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	8	5	theme	various	1259:1265	arg1	H-bonding					1333:1341	H-bonding	1333:1341	H-bonding	1333:1341	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	8	5	theme	various	1259:1265	arg1	stacking					1319:1326	n-π stacking	1315:1326	n-π stacking	1315:1326	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	8	5	theme	various	1259:1265	arg1	attraction					1303:1312	electrostatic attraction	1289:1312	electrostatic attraction	1289:1312	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	8	5	theme	various	1259:1265	arg1	interactions					1267:1278	various interactions	1259:1278	various interactions	1259:1278	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	0	6	theme	dye	90:92	arg1	removal					62:68	the removal	58:68	the removal of reactive red 120 dye	58:92	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	6	7	theme	Freundlich	1042:1051	arg1	model					1053:1057	the Freundlich model	1038:1057	the Freundlich model	1038:1057	The adsorption was well described by the Freundlich model and pseudo-second order kinetic model.					
31760013	5	8	theme	dye	879:881	arg1	concentration					883:895	RR120 dye concentration	873:895	RR120 dye concentration (30-400 mg/L)	873:909	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	8	theme	dye	879:881	arg1	30-400 mg/L					898:908	30-400 mg/L	898:908	30-400 mg/L	898:908	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	9	theme	RR120	873:877	arg1	concentration					883:895	RR120 dye concentration	873:895	RR120 dye concentration (30-400 mg/L)	873:909	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	9	theme	RR120	873:877	arg1	30-400 mg/L					898:908	30-400 mg/L	898:908	30-400 mg/L	898:908	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	1	10	theme	reactive	245:252	arg1	dye					270:272	reactive red 120 (RR120) dye	245:272	reactive red 120 (RR120) dye	245:272	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	8	11	theme	CS-GLA/TNC-25	1218:1230	arg1	surface					1232:1238	the CS-GLA/TNC-25 surface	1214:1238	the CS-GLA/TNC-25 surface	1214:1238	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	4	12	theme	best	638:641	arg1	property					654:661	the best adsorptive property	634:661	the best adsorptive property	634:661	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	3	13	from	CS-GLA/TNC-50	538:550	arg1	solution					585:592	the aqueous solution	573:592	the aqueous solution	573:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	1	14	theme	red	254:256	arg1	dye					270:272	reactive red 120 (RR120) dye	245:272	reactive red 120 (RR120) dye	245:272	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	0	15	theme	Adsorption	95:104	arg1	study					120:124	Adsorption and mechanism study	95:124	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.	0:125	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	5	16	theme	batch	978:982	arg1	mode					995:998	batch adsorption mode	978:998	batch adsorption mode	978:998	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	4	17	theme	composite	770:778	arg1	formulation					780:790	the composite formulation	766:790	the composite formulation	766:790	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	4	18	theme	area	725:728	arg1	groups					756:761	the surface area and available amine (NH2) groups	713:761	the surface area and available amine (NH2) groups	713:761	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	1	19	theme	tunable	142:148	arg1	CS-GLA					202:207	CS-GLA	202:207	CS-GLA	202:207	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	1	19	theme	tunable	142:148	arg1	chitosan-glutaraldehyde					177:199	tunable Schiff's base-cross-linked chitosan-glutaraldehyde	142:199	tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA)	142:208	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	5	20	theme	adsorption	984:993	arg1	mode					995:998	batch adsorption mode	978:998	batch adsorption mode	978:998	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	1	21	link	base-cross-linked	159:175	arg1	CS-GLA					202:207	CS-GLA	202:207	CS-GLA	202:207	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	1	21	link	base-cross-linked	159:175	arg1	chitosan-glutaraldehyde					177:199	tunable Schiff's base-cross-linked chitosan-glutaraldehyde	142:199	tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA)	142:208	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	4	22	from	balancing	695:703	arg1	formulation					780:790	the composite formulation	766:790	the composite formulation	766:790	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	2	23	theme	50	391:392	arg1	%					393:393	%	393:393	%	393:393	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	2	24	theme	25	348:349	arg1	%					350:350	%	350:350	%	350:350	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	2	25	theme	%	393:393	arg1	TiO2					395:398	50% TiO2	391:398	50% TiO2 nanoparticles (CS-GLA/TNC-50)	391:428	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	7	26	theme	adsorption	1102:1111	arg1	capacity					1113:1120	The adsorption capacity	1098:1120	The adsorption capacity of CS-GLA/TNC-25 for RR120 dye	1098:1151	The adsorption capacity of CS-GLA/TNC-25 for RR120 dye was 103.1 mg/g at 303K.					
31760013	8	27	theme	n-π	1315:1317	arg1	stacking					1319:1326	n-π stacking	1315:1326	n-π stacking	1315:1326	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	0	28	theme	Tunable	0:6	arg1	composite					44:52	Tunable Schiff's base-cross-linked chitosan composite	0:52	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.	0:125	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	6	29	theme	kinetic	1083:1089	arg1	model					1091:1095	pseudo-second order kinetic model	1063:1095	pseudo-second order kinetic model	1063:1095	The adsorption was well described by the Freundlich model and pseudo-second order kinetic model.					
31760013	8	30	from	RR120	1205:1209	arg1	surface					1232:1238	the CS-GLA/TNC-25 surface	1214:1238	the CS-GLA/TNC-25 surface	1214:1238	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	3	31	theme	CS-GLA	511:516	arg1	properties					497:506	The adsorptive properties	482:506	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution	482:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	1	32	theme	base-cross-linked	159:175	arg1	CS-GLA					202:207	CS-GLA	202:207	CS-GLA	202:207	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	1	32	theme	base-cross-linked	159:175	arg1	chitosan-glutaraldehyde					177:199	tunable Schiff's base-cross-linked chitosan-glutaraldehyde	142:199	tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA)	142:208	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	0	33	theme	base-cross-linked	17:33	arg1	composite					44:52	Tunable Schiff's base-cross-linked chitosan composite	0:52	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.	0:125	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	5	34	theme	solution	912:919	arg1	3-12					925:928	3-12	925:928	3-12	925:928	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	34	theme	solution	912:919	arg1	pH					921:922	solution pH	912:922	solution pH (3-12)	912:929	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	0	35	theme	mechanism	110:118	arg1	study					120:124	Adsorption and mechanism study	95:124	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.	0:125	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	3	36	theme	adsorptive	486:495	arg1	properties					497:506	The adsorptive properties	482:506	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution	482:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	3	37	from	CS-GLA/TNC-25	519:531	arg1	solution					585:592	the aqueous solution	573:592	the aqueous solution	573:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	5	38	theme	adsorbent	842:850	arg1	0.01-1.2 g					860:869	0.01-1.2 g	860:869	0.01-1.2 g	860:869	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	38	theme	adsorbent	842:850	arg1	dosage					852:857	adsorbent dosage	842:857	adsorbent dosage (0.01-1.2 g)	842:870	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	1	39	theme	RR120	263:267	arg1	dye					270:272	reactive red 120 (RR120) dye	245:272	reactive red 120 (RR120) dye	245:272	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	9	40	theme	textile	1464:1470	arg1	dyes					1472:1475	textile dyes	1464:1475	textile dyes	1464:1475	Results indicate the potential application of CS-GLA/TNC-25 as environment-friendly biosorbent for removing acid and/or textile dyes, such as RR120, from aqueous environments.					
31760013	9	40	theme	textile	1464:1470	arg1	RR120					1486:1490	RR120	1486:1490	RR120	1486:1490	Results indicate the potential application of CS-GLA/TNC-25 as environment-friendly biosorbent for removing acid and/or textile dyes, such as RR120, from aqueous environments.					
31760013	6	41	theme	order	1077:1081	arg1	model					1091:1095	pseudo-second order kinetic model	1063:1095	pseudo-second order kinetic model	1063:1095	The adsorption was well described by the Freundlich model and pseudo-second order kinetic model.					
31760013	4	42	theme	perfect	687:693	arg1	balancing					695:703	the perfect balancing	683:703	the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation	683:790	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	8	43	theme	RR120	1205:1209	arg1	mechanism					1192:1200	The adsorption mechanism	1177:1200	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface	1177:1238	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	4	44	theme	adsorptive	643:652	arg1	property					654:661	the best adsorptive property	634:661	the best adsorptive property	634:661	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	3	45	theme	aqueous	577:583	arg1	solution					585:592	the aqueous solution	573:592	the aqueous solution	573:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	5	46	theme	adsorption	807:816	arg1	time					944:947	contact time	936:947	contact time (0-400 min)	936:959	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	46	theme	adsorption	807:816	arg1	concentration					883:895	RR120 dye concentration	873:895	RR120 dye concentration (30-400 mg/L)	873:909	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	46	theme	adsorption	807:816	arg1	pH					921:922	solution pH	912:922	solution pH (3-12)	912:929	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	46	theme	adsorption	807:816	arg1	dosage					852:857	adsorbent dosage	842:857	adsorbent dosage (0.01-1.2 g)	842:870	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	46	theme	adsorption	807:816	arg1	parameters					822:831	adsorption key parameters	807:831	adsorption key parameters	807:831	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	0	47	theme	chitosan	35:42	arg1	composite					44:52	Tunable Schiff's base-cross-linked chitosan composite	0:52	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.	0:125	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	2	48	theme	molecular	461:469	arg1	structure					471:479	the CS-GLA's molecular structure	448:479	the CS-GLA's molecular structure	448:479	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	4	49	theme	NH2	751:753	arg1	groups					756:761	the surface area and available amine (NH2) groups	713:761	the surface area and available amine (NH2) groups	713:761	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	8	50	theme	adsorption	1181:1190	arg1	mechanism					1192:1200	The adsorption mechanism	1177:1200	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface	1177:1238	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	2	51	theme	TiO2	352:355	arg1	nanoparticles					357:369	25% TiO2 nanoparticles	348:369	25% TiO2 nanoparticles (CS-GLA/TNC-25)	348:385	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	2	51	theme	TiO2	352:355	arg1	CS-GLA/TNC-25					372:384	CS-GLA/TNC-25	372:384	CS-GLA/TNC-25	372:384	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	0	52	dep	composite	44:52	arg1	study					120:124	Adsorption and mechanism study	95:124	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.	0:125	Tunable Schiff's base-cross-linked chitosan composite for the removal of reactive red 120 dye: Adsorption and mechanism study.					
31760013	2	53	theme	TiO2	395:398	arg1	nanoparticles					400:412	50% TiO2 nanoparticles	391:412	50% TiO2 nanoparticles (CS-GLA/TNC-50)	391:428	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	2	53	theme	TiO2	395:398	arg1	CS-GLA/TNC-50					415:427	CS-GLA/TNC-50	415:427	CS-GLA/TNC-50	415:427	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	2	54	theme	%	350:350	arg1	nanoparticles					357:369	25% TiO2 nanoparticles	348:369	25% TiO2 nanoparticles (CS-GLA/TNC-25)	348:385	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	2	54	theme	%	350:350	arg1	CS-GLA/TNC-25					372:384	CS-GLA/TNC-25	372:384	CS-GLA/TNC-25	372:384	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	3	55	theme	CS-GLA/TNC-25	519:531	arg1	properties					497:506	The adsorptive properties	482:506	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution	482:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	2	56	theme	Different	300:308	arg1	ratios					310:315	Different ratios	300:315	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50),	300:429	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	1	57	theme	aqueous	282:288	arg1	solution					290:297	an aqueous solution	279:297	an aqueous solution	279:297	In this study, tunable Schiff's base-cross-linked chitosan-glutaraldehyde (CS-GLA) was modified and applied to remove reactive red 120 (RR120) dye from an aqueous solution.					
31760013	9	58	theme	aqueous	1498:1504	arg1	environments					1506:1517	aqueous environments	1498:1517	aqueous environments	1498:1517	Results indicate the potential application of CS-GLA/TNC-25 as environment-friendly biosorbent for removing acid and/or textile dyes, such as RR120, from aqueous environments.					
31760013	5	59	theme	contact	936:942	arg1	time					944:947	contact time	936:947	contact time (0-400 min)	936:959	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	59	theme	contact	936:942	arg1	0-400 min					950:958	0-400 min	950:958	0-400 min	950:958	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	4	60	theme	available	734:742	arg1	amine					744:748	available amine	734:748	available amine	734:748	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	9	61	theme	CS-GLA/TNC-25	1390:1402	arg1	application					1375:1385	the potential application	1361:1385	the potential application of CS-GLA/TNC-25	1361:1402	Results indicate the potential application of CS-GLA/TNC-25 as environment-friendly biosorbent for removing acid and/or textile dyes, such as RR120, from aqueous environments.					
31760013	3	62	from	properties	497:506	arg1	solution					585:592	the aqueous solution	573:592	the aqueous solution	573:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	4	63	theme	surface	717:723	arg1	area					725:728	surface area	717:728	surface area	717:728	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	3	64	theme	RR120	560:564	arg1	dye					566:568	the RR120 dye	556:568	the RR120 dye	556:568	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	5	65	theme	parameters	822:831	arg1	impact					797:802	The impact	793:802	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min)	793:959	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	8	66	from	surface	1232:1238	arg1	mechanism					1192:1200	The adsorption mechanism	1177:1200	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface	1177:1238	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	8	67	theme	electrostatic	1289:1301	arg1	attraction					1303:1312	electrostatic attraction	1289:1312	electrostatic attraction	1289:1312	The adsorption mechanism of RR120 on the CS-GLA/TNC-25 surface can be assigned to various interactions, such as electrostatic attraction, n-π stacking, and H-bonding.					
31760013	3	68	from	solution	585:592	arg1	properties					497:506	The adsorptive properties	482:506	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution	482:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	9	69	theme	potential	1365:1373	arg1	application					1375:1385	the potential application	1361:1385	the potential application of CS-GLA/TNC-25	1361:1402	Results indicate the potential application of CS-GLA/TNC-25 as environment-friendly biosorbent for removing acid and/or textile dyes, such as RR120, from aqueous environments.					
31760013	3	70	theme	CS-GLA/TNC-50	538:550	arg1	properties					497:506	The adsorptive properties	482:506	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution	482:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	5	71	theme	key	818:820	arg1	time					944:947	contact time	936:947	contact time (0-400 min)	936:959	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	71	theme	key	818:820	arg1	concentration					883:895	RR120 dye concentration	873:895	RR120 dye concentration (30-400 mg/L)	873:909	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	71	theme	key	818:820	arg1	pH					921:922	solution pH	912:922	solution pH (3-12)	912:929	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	71	theme	key	818:820	arg1	dosage					852:857	adsorbent dosage	842:857	adsorbent dosage (0.01-1.2 g)	842:870	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	5	71	theme	key	818:820	arg1	parameters					822:831	adsorption key parameters	807:831	adsorption key parameters	807:831	The impact of adsorption key parameters, such as adsorbent dosage (0.01-1.2 g), RR120 dye concentration (30-400 mg/L), solution pH (3-12), and contact time (0-400 min) were explored by batch adsorption mode.					
31760013	3	72	from	CS-GLA	511:516	arg1	solution					585:592	the aqueous solution	573:592	the aqueous solution	573:592	The adsorptive properties of CS-GLA, CS-GLA/TNC-25, and CS-GLA/TNC-50 for the RR120 dye in the aqueous solution were evaluated.					
31760013	4	73	theme	amine	744:748	arg1	groups					756:761	the surface area and available amine (NH2) groups	713:761	the surface area and available amine (NH2) groups	713:761	CS-GLA/TNC-25 exhibited the best adsorptive property possibly because of the perfect balancing between the surface area and available amine (NH2) groups in the composite formulation.					
31760013	6	74	theme	pseudo-second	1063:1075	arg1	model					1091:1095	pseudo-second order kinetic model	1063:1095	pseudo-second order kinetic model	1063:1095	The adsorption was well described by the Freundlich model and pseudo-second order kinetic model.					
31760013	2	75	theme	nanoparticles	325:337	arg1	ratios					310:315	Different ratios	300:315	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50),	300:429	Different ratios of TiO2 nanoparticles, such as 25% TiO2 nanoparticles (CS-GLA/TNC-25) and 50% TiO2 nanoparticles (CS-GLA/TNC-50), were loaded into the CS-GLA's molecular structure.					
31760013	7	76	theme	CS-GLA/TNC-25	1125:1137	arg1	capacity					1113:1120	The adsorption capacity	1098:1120	The adsorption capacity of CS-GLA/TNC-25 for RR120 dye	1098:1151	The adsorption capacity of CS-GLA/TNC-25 for RR120 dye was 103.1 mg/g at 303K.					
31490908	8	0	theme	proliferative	1671:1683	arg1	keratinocytes					1685:1697	proliferative keratinocytes	1671:1697	proliferative keratinocytes	1671:1697	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	6	1	from	RESULTS	1126:1132	arg1	group					1153:1157	the upside-down group	1137:1157	the upside-down group	1137:1157	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	8	2	from	grafting	1511:1518	arg1	membranes					1620:1628	esterified hyaluronic acid membranes	1593:1628	esterified hyaluronic acid membranes	1593:1628	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	4	3	theme	keratinocytes	597:609	arg1	transfer					579:586	the transfer	575:586	the transfer of human keratinocytes directly to the recipient wound bed	575:645	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	3	4	theme	biomaterials	508:519	arg1	use					413:415	use	413:415	use of cell monolayers instead of a fully differentiated epithelium	413:479	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	3	4	theme	biomaterials	508:519	arg1	use					493:495	use	493:495	use of various biomaterials to grow and transport the cultured cells	493:560	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	9	5	from	dish	1905:1908	arg1	cells					1882:1886	cells	1882:1886	cells from the culture dish	1882:1908	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	9	5	from	dish	1905:1908	arg1	detachment					1868:1877	enzymatic detachment	1858:1877	enzymatic detachment of cells from the culture dish	1858:1908	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	6	6	dep	multi-layered	1186:1198	arg1	differentiating					1201:1215	differentiating	1201:1215	differentiating	1201:1215	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	6	7	theme	control	1291:1297	arg1	group					1299:1303	the control group	1287:1303	the control group	1287:1303	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	2	8	theme	cultured	277:284	arg1	autografts					297:306	cultured epithelial autografts	277:306	cultured epithelial autografts	277:306	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	5	9	from	wounds	1047:1052	arg1	controls					1082:1089	controls	1082:1089	controls with the cell-free membrane alone	1082:1123	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	9	from	wounds	1047:1052	arg1	mice					1070:1073	athymic nude mice	1057:1073	athymic nude mice	1057:1073	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	9	10	theme	production	1936:1945	arg1	steps					1947:1951	production steps	1936:1951	production steps required	1936:1960	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	4	11	theme	upside-down	660:670	arg1	technique					673:681	an "upside-down" technique	656:681	an "upside-down" technique	656:681	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	2	12	theme	various	325:331	arg1	efforts					333:339	various efforts	325:339	various efforts	325:339	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	9	13	theme	grafting	1752:1759	arg1	grafts					1813:1818	CEA sheet grafts	1803:1818	CEA sheet grafts	1803:1818	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	9	13	theme	grafting	1752:1759	arg1	protocols					1761:1769	standard grafting protocols	1743:1769	standard grafting protocols	1743:1769	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	6	14	theme	multi-layered	1186:1198	arg1	grafting					1174:1181	grafting	1174:1181	grafting	1174:1181	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	6	14	theme	multi-layered	1186:1198	arg1	epidermis					1217:1225	a multi-layered, differentiating epidermis	1184:1225	a multi-layered, differentiating epidermis	1184:1225	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	5	15	from	monolayers	834:843	arg1	membranes					875:883	esterified hyaluronic acid membranes	848:883	esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	848:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	16	theme	human	815:819	arg1	monolayers					834:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers	766:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	766:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	16	theme	human	815:819	arg1	grafts					984:989	upside-down grafts	972:989	upside-down grafts	972:989	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	16	theme	human	815:819	arg1	grafts					1007:1012	upside-up grafts	997:1012	upside-up grafts	997:1012	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	17	theme	hyaluronic	859:868	arg1	acid					870:873	esterified hyaluronic acid	848:873	esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	848:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	9	18	theme	epithelium	1783:1792	arg1	grafts					1813:1818	CEA sheet grafts	1803:1818	CEA sheet grafts	1803:1818	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	9	18	theme	epithelium	1783:1792	arg1	protocols					1761:1769	standard grafting protocols	1743:1769	standard grafting protocols	1743:1769	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	6	19	theme	upside-up	1264:1272	arg1	group					1274:1278	the upside-up group	1260:1278	the upside-up group	1260:1278	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	4	20	with	wound	726:730	arg1	material					749:756	the carrier material	737:756	the carrier material	737:756	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	2	21	theme	clinical	231:238	arg1	drawbacks					240:248	several clinical drawbacks	223:248	several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts)	223:307	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	4	22	used	used	651:654	arg2	we					648:649	we	648:649	we	648:649	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	5	23	theme	upside-down	972:982	arg1	grafts					984:989	upside-down grafts	972:989	upside-down grafts	972:989	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	23	theme	upside-down	972:982	arg1	monolayers					834:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers	766:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	766:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	9	24	theme	CEA	1803:1805	arg1	grafts					1813:1818	CEA sheet grafts	1803:1818	CEA sheet grafts	1803:1818	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	7	25	theme	keratinocytes	1367:1379	arg1	Persistence					1346:1356	Persistence	1346:1356	Persistence of human keratinocytes	1346:1379	Persistence of human keratinocytes was shown in the upside-down group only, from day 7 until day 35 after grafting.					
31490908	0	26	theme	Upside-Down	57:67	arg1	Grafts					70:75	"Upside-Down" Grafts	56:75	"Upside-Down" Grafts	56:75	Keratinocyte Monolayers on Hyaluronic Acid Membranes as "Upside-Down" Grafts Reconstitute Full-Thickness Wounds.					
31490908	2	27	theme	autografts	259:268	arg1	drawbacks					240:248	several clinical drawbacks	223:248	several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts)	223:307	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	1	28	theme	cultured	153:160	arg1	keratinocytes					173:185	cultured epithelial keratinocytes	153:185	cultured epithelial keratinocytes	153:185	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes is a well-accepted method.					
31490908	8	29	theme	subconfluent	1523:1534	arg1	monolayers					1536:1545	subconfluent monolayers	1523:1545	subconfluent monolayers of serum-free cultured human keratinocytes	1523:1588	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	6	30	from	wounds	1250:1255	arg1	group					1299:1303	the control group	1287:1303	the control group	1287:1303	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	6	30	from	wounds	1250:1255	arg1	group					1274:1278	the upside-up group	1260:1278	the upside-up group	1260:1278	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	0	31	theme	Full-Thickness	90:103	arg1	Wounds					105:110	Full-Thickness Wounds	90:110	Full-Thickness Wounds	90:110	Keratinocyte Monolayers on Hyaluronic Acid Membranes as "Upside-Down" Grafts Reconstitute Full-Thickness Wounds.					
31490908	8	32	dep	means	1644:1648	arg1	transfer					1653:1660	transfer	1653:1660	to transfer actively proliferative keratinocytes	1650:1697	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	8	32	dep	means	1644:1648	arg1	reduces					1704:1710	reduces	1704:1710	reduces wound contraction	1704:1728	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	5	33	theme	full-thickness	1032:1045	arg1	wounds					1047:1052	standardized full-thickness wounds	1019:1052	standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone	1019:1123	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	7	34	theme	human	1361:1365	arg1	keratinocytes					1367:1379	human keratinocytes	1361:1379	human keratinocytes	1361:1379	Persistence of human keratinocytes was shown in the upside-down group only, from day 7 until day 35 after grafting.					
31490908	8	35	theme	cultured	1561:1568	arg1	keratinocytes					1576:1588	serum-free cultured human keratinocytes	1550:1588	serum-free cultured human keratinocytes	1550:1588	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	0	36	theme	Keratinocyte	0:11	arg1	Monolayers					13:22	Keratinocyte Monolayers	0:22	Keratinocyte Monolayers on Hyaluronic Acid Membranes as "Upside-Down" Grafts	0:75	Keratinocyte Monolayers on Hyaluronic Acid Membranes as "Upside-Down" Grafts Reconstitute Full-Thickness Wounds.					
31490908	4	37	from	wound	726:730	arg1	top					761:763	top	761:763	top	761:763	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	3	38	theme	Recent	385:390	arg1	developments					392:403	Recent developments	385:403	Recent developments	385:403	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	8	39	theme	keratinocytes	1576:1588	arg1	monolayers					1536:1545	subconfluent monolayers	1523:1545	subconfluent monolayers of serum-free cultured human keratinocytes	1523:1588	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	5	40	theme	keratinocyte	821:832	arg1	monolayers					834:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers	766:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	766:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	40	theme	keratinocyte	821:832	arg1	grafts					984:989	upside-down grafts	972:989	upside-down grafts	972:989	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	40	theme	keratinocyte	821:832	arg1	grafts					1007:1012	upside-up grafts	997:1012	upside-up grafts	997:1012	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	41	theme	upside-up	997:1005	arg1	monolayers					834:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers	766:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	766:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	41	theme	upside-up	997:1005	arg1	grafts					1007:1012	upside-up grafts	997:1012	upside-up grafts	997:1012	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	3	42	theme	monolayers	425:434	arg1	use					413:415	use	413:415	use of cell monolayers instead of a fully differentiated epithelium	413:479	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	3	42	theme	monolayers	425:434	arg1	use					493:495	use	493:495	use of various biomaterials to grow and transport the cultured cells	493:560	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	8	43	theme	esterified	1593:1602	arg1	acid					1615:1618	esterified hyaluronic acid	1593:1618	esterified hyaluronic acid membranes	1593:1628	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	3	44	theme	epithelium	470:479	arg1	use					413:415	use	413:415	use of cell monolayers instead of a fully differentiated epithelium	413:479	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	3	44	theme	epithelium	470:479	arg1	use					493:495	use	493:495	use of various biomaterials to grow and transport the cultured cells	493:560	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	0	45	theme	Acid	38:41	arg1	Membranes					43:51	Hyaluronic Acid Membranes	27:51	Hyaluronic Acid Membranes as "Upside-Down" Grafts	27:75	Keratinocyte Monolayers on Hyaluronic Acid Membranes as "Upside-Down" Grafts Reconstitute Full-Thickness Wounds.					
31490908	5	46	theme	MATERIAL	766:773	arg1	monolayers					834:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers	766:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	766:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	46	theme	MATERIAL	766:773	arg1	grafts					984:989	upside-down grafts	972:989	upside-down grafts	972:989	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	46	theme	MATERIAL	766:773	arg1	grafts					1007:1012	upside-up grafts	997:1012	upside-up grafts	997:1012	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	8	47	theme	acid	1615:1618	arg1	membranes					1620:1628	esterified hyaluronic acid membranes	1593:1628	esterified hyaluronic acid membranes	1593:1628	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	5	48	theme	METHODS	779:785	arg1	monolayers					834:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers	766:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	766:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	48	theme	METHODS	779:785	arg1	grafts					984:989	upside-down grafts	972:989	upside-down grafts	972:989	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	48	theme	METHODS	779:785	arg1	grafts					1007:1012	upside-up grafts	997:1012	upside-up grafts	997:1012	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	9	49	theme	cells	1882:1886	arg1	detachment					1868:1877	enzymatic detachment	1858:1877	enzymatic detachment of cells from the culture dish	1858:1908	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	8	50	theme	suitable	1635:1642	arg1	grafting					1511:1518	upside-down grafting	1499:1518	upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes	1499:1628	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	8	50	theme	suitable	1635:1642	arg1	means					1644:1648	a suitable means	1633:1648	a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction	1633:1728	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	8	51	theme	wound	1712:1716	arg1	contraction					1718:1728	wound contraction	1712:1728	wound contraction	1712:1728	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	9	52	from	detachment	1868:1877	arg1	dish					1905:1908	the culture dish	1893:1908	the culture dish	1893:1908	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	5	53	theme	nude	1065:1068	arg1	mice					1070:1073	athymic nude mice	1057:1073	athymic nude mice	1057:1073	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	2	54	theme	cell	370:373	arg1	delivery					375:382	cell delivery	370:382	cell delivery	370:382	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	6	55	theme	upside-down	1141:1151	arg1	group					1153:1157	the upside-down group	1137:1157	the upside-down group	1137:1157	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	5	56	with	controls	1082:1089	arg1	membrane					1110:1117	the cell-free membrane	1096:1117	the cell-free membrane alone	1096:1123	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	3	57	theme	cultured	547:554	arg1	cells					556:560	the cultured cells	543:560	the cultured cells	543:560	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	4	58	theme	human	591:595	arg1	keratinocytes					597:609	human keratinocytes	591:609	human keratinocytes	591:609	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	2	59	theme	epithelial	286:295	arg1	autografts					297:306	cultured epithelial autografts	277:306	cultured epithelial autografts	277:306	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	5	60	theme	acid	870:873	arg1	membranes					875:883	esterified hyaluronic acid membranes	848:883	esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	848:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	2	61	dep	autografts	259:268	arg1	autografts					297:306	cultured epithelial autografts	277:306	cultured epithelial autografts	277:306	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	2	61	dep	autografts	259:268	arg1	CEA					271:273	CEA	271:273	CEA	271:273	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	1	62	theme	well-accepted	192:204	arg1	method					206:211	a well-accepted method	190:211	a well-accepted method	190:211	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes is a well-accepted method.					
31490908	1	62	theme	well-accepted	192:204	arg1	replacement					129:139	BACKGROUND Skin replacement	113:139	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes	113:185	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes is a well-accepted method.					
31490908	8	63	theme	monolayers	1536:1545	arg1	grafting					1511:1518	upside-down grafting	1499:1518	upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes	1499:1628	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	8	63	theme	monolayers	1536:1545	arg1	means					1644:1648	a suitable means	1633:1648	a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction	1633:1728	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	9	64	theme	standard	1743:1750	arg1	grafts					1813:1818	CEA sheet grafts	1803:1818	CEA sheet grafts	1803:1818	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	9	64	theme	standard	1743:1750	arg1	protocols					1761:1769	standard grafting protocols	1743:1769	standard grafting protocols	1743:1769	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	9	65	theme	steps	1947:1951	arg1	number					1926:1931	the number	1922:1931	the number of production steps required	1922:1960	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	6	66	dep	day	1338:1340	arg1	up					1332:1333	up	1332:1333	up	1332:1333	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	4	67	theme	wound	637:641	arg1	bed					643:645	the recipient wound bed	623:645	the recipient wound bed	623:645	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	2	68	theme	cell	353:356	arg1	culture					358:364	cell culture	353:364	cell culture	353:364	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	2	69	theme	sheet	253:257	arg1	autografts					259:268	sheet autografts	253:268	sheet autografts (CEA - cultured epithelial autografts)	253:307	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	5	70	theme	esterified	848:857	arg1	acid					870:873	esterified hyaluronic acid	848:873	esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	848:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	9	71	theme	cultured	1774:1781	arg1	epithelium					1783:1792	cultured epithelium	1774:1792	cultured epithelium	1774:1792	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	6	72	dep	found	1231:1235	arg1	whereas					1238:1244	whereas	1238:1244	whereas	1238:1244	RESULTS In the upside-down group, 14 days after grafting, a multi-layered, differentiating epidermis was found, whereas the wounds in the upside-up group and in the control group were not completely closed up to day 21.					
31490908	1	73	theme	Skin	124:127	arg1	method					206:211	a well-accepted method	190:211	a well-accepted method	190:211	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes is a well-accepted method.					
31490908	1	73	theme	Skin	124:127	arg1	replacement					129:139	BACKGROUND Skin replacement	113:139	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes	113:185	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes is a well-accepted method.					
31490908	7	74	theme	upside-down	1398:1408	arg1	group					1410:1414	the upside-down group	1394:1414	the upside-down group only	1394:1419	Persistence of human keratinocytes was shown in the upside-down group only, from day 7 until day 35 after grafting.					
31490908	4	75	theme	cultured	695:702	arg1	cells					704:708	cultured cells	695:708	cultured cells	695:708	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	5	76	theme	cell-free	1100:1108	arg1	membrane					1110:1117	the cell-free membrane	1096:1117	the cell-free membrane alone	1096:1123	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	8	77	theme	upside-down	1499:1509	arg1	grafting					1511:1518	upside-down grafting	1499:1518	upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes	1499:1628	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	8	77	theme	upside-down	1499:1509	arg1	means					1644:1648	a suitable means	1633:1648	a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction	1633:1728	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	0	78	theme	"	68:68	arg1	Grafts					70:75	"Upside-Down" Grafts	56:75	"Upside-Down" Grafts	56:75	Keratinocyte Monolayers on Hyaluronic Acid Membranes as "Upside-Down" Grafts Reconstitute Full-Thickness Wounds.					
31490908	9	79	theme	sheet	1807:1811	arg1	grafts					1813:1818	CEA sheet grafts	1803:1818	CEA sheet grafts	1803:1818	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	4	80	theme	"	671:671	arg1	technique					673:681	an "upside-down" technique	656:681	an "upside-down" technique	656:681	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	1	81	theme	epithelial	162:171	arg1	keratinocytes					173:185	cultured epithelial keratinocytes	153:185	cultured epithelial keratinocytes	153:185	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes is a well-accepted method.					
31490908	0	82	from	Monolayers	13:22	arg1	Membranes					43:51	Hyaluronic Acid Membranes	27:51	Hyaluronic Acid Membranes as "Upside-Down" Grafts	27:75	Keratinocyte Monolayers on Hyaluronic Acid Membranes as "Upside-Down" Grafts Reconstitute Full-Thickness Wounds.					
31490908	8	83	theme	serum-free	1550:1559	arg1	keratinocytes					1576:1588	serum-free cultured human keratinocytes	1550:1588	serum-free cultured human keratinocytes	1550:1588	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	4	84	theme	carrier	741:747	arg1	material					749:756	the carrier material	737:756	the carrier material	737:756	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31490908	5	85	theme	standardized	1019:1030	arg1	wounds					1047:1052	standardized full-thickness wounds	1019:1052	standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone	1019:1123	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	8	86	theme	human	1570:1574	arg1	keratinocytes					1576:1588	serum-free cultured human keratinocytes	1550:1588	serum-free cultured human keratinocytes	1550:1588	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	3	87	theme	differentiated	455:468	arg1	epithelium					470:479	a fully differentiated epithelium	447:479	a fully differentiated epithelium	447:479	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	5	88	theme	Subconfluent	787:798	arg1	monolayers					834:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers	766:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	766:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	88	theme	Subconfluent	787:798	arg1	grafts					984:989	upside-down grafts	972:989	upside-down grafts	972:989	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	88	theme	Subconfluent	787:798	arg1	grafts					1007:1012	upside-up grafts	997:1012	upside-up grafts	997:1012	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	0	89	theme	Hyaluronic	27:36	arg1	Membranes					43:51	Hyaluronic Acid Membranes	27:51	Hyaluronic Acid Membranes as "Upside-Down" Grafts	27:75	Keratinocyte Monolayers on Hyaluronic Acid Membranes as "Upside-Down" Grafts Reconstitute Full-Thickness Wounds.					
31490908	5	90	theme	second-passage	800:813	arg1	monolayers					834:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers	766:843	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites)	766:942	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	90	theme	second-passage	800:813	arg1	grafts					984:989	upside-down grafts	972:989	upside-down grafts	972:989	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	90	theme	second-passage	800:813	arg1	grafts					1007:1012	upside-up grafts	997:1012	upside-up grafts	997:1012	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	8	91	theme	hyaluronic	1604:1613	arg1	acid					1615:1618	esterified hyaluronic acid	1593:1618	esterified hyaluronic acid membranes	1593:1628	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	9	92	theme	enzymatic	1858:1866	arg1	detachment					1868:1877	enzymatic detachment	1858:1877	enzymatic detachment of cells from the culture dish	1858:1908	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	8	93	dep	CONCLUSIONS	1462:1472	arg1	confirms					1485:1492	confirms	1485:1492	confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction	1485:1728	CONCLUSIONS This study confirms that upside-down grafting of subconfluent monolayers of serum-free cultured human keratinocytes on esterified hyaluronic acid membranes is a suitable means to transfer actively proliferative keratinocytes, and reduces wound contraction.					
31490908	3	94	theme	cell	420:423	arg1	monolayers					425:434	cell monolayers	420:434	cell monolayers	420:434	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	2	95	theme	several	223:229	arg1	drawbacks					240:248	several clinical drawbacks	223:248	several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts)	223:307	However, several clinical drawbacks of sheet autografts (CEA - cultured epithelial autografts) have stimulated various efforts to optimize cell culture and cell delivery.					
31490908	5	96	dep	membranes	875:883	arg1	Keratinocyte-Hyaluronic-Acid-Membrane-Composites					894:941	Keratinocyte-Hyaluronic-Acid-Membrane-Composites	894:941	Keratinocyte-Hyaluronic-Acid-Membrane-Composites	894:941	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	5	96	dep	membranes	875:883	arg1	KHAMC					886:890	KHAMC	886:890	KHAMC	886:890	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	3	97	theme	various	500:506	arg1	biomaterials					508:519	various biomaterials	500:519	various biomaterials	500:519	Recent developments include use of cell monolayers instead of a fully differentiated epithelium, as well as use of various biomaterials to grow and transport the cultured cells.					
31490908	1	98	theme	BACKGROUND	113:122	arg1	method					206:211	a well-accepted method	190:211	a well-accepted method	190:211	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes is a well-accepted method.					
31490908	1	98	theme	BACKGROUND	113:122	arg1	replacement					129:139	BACKGROUND Skin replacement	113:139	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes	113:185	BACKGROUND Skin replacement by means of cultured epithelial keratinocytes is a well-accepted method.					
31490908	5	99	theme	athymic	1057:1063	arg1	mice					1070:1073	athymic nude mice	1057:1073	athymic nude mice	1057:1073	MATERIAL AND METHODS Subconfluent second-passage human keratinocyte monolayers on esterified hyaluronic acid membranes (KHAMC - Keratinocyte-Hyaluronic-Acid-Membrane-Composites) were transplanted either as upside-down grafts or as upside-up grafts onto standardized full-thickness wounds in athymic nude mice versus controls with the cell-free membrane alone.					
31490908	9	100	theme	culture	1897:1903	arg1	dish					1905:1908	the culture dish	1893:1908	the culture dish	1893:1908	Compared to standard grafting protocols of cultured epithelium, such as CEA sheet grafts, it is easier to apply, does not need enzymatic detachment of cells from the culture dish, and limits the number of production steps required.					
31490908	4	101	theme	recipient	627:635	arg1	bed					643:645	the recipient wound bed	623:645	the recipient wound bed	623:645	To optimize the transfer of human keratinocytes directly to the recipient wound bed, we used an "upside-down" technique, delivering cultured cells directly to the wound with the carrier material on top.					
31665090	8	0	theme	bone	1015:1018	arg1	marrow					1020:1025	the bone marrow	1011:1025	the bone marrow	1011:1025	By flow cytometry, we demonstrated that upon administration of RD-LPS, the number of bone marrow-derived endothelial progenitor cells increased in the bone marrow and, in particular, in the circulation.					
31665090	11	1	theme	radio-detoxified	1456:1471	arg1	treatment					1477:1485	radio-detoxified LPS treatment	1456:1485	radio-detoxified LPS treatment	1456:1485	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	13	2	theme	in	1954:1955	arg1	experiments					1963:1973	in vitro experiments	1954:1973	in vitro experiments	1954:1973	In contrast to these in vivo results, in vitro experiments did not support the effect of sEVs on EPCs.					
31665090	2	3	theme	variety	394:400	arg1	treatment					379:387	the treatment	375:387	the treatment of a variety of disorders	375:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	13	4	dep	in	1937:1938	arg1	vivo					1940:1943	vivo	1940:1943	vivo	1940:1943	In contrast to these in vivo results, in vitro experiments did not support the effect of sEVs on EPCs.					
31665090	5	5	theme	irradiation	703:713	arg1	effects					686:692	the effects	682:692	the effects of local irradiation	682:713	In a chest-irradiated mouse model of cardiac injury, we investigated the effects of local irradiation.					
31665090	10	6	theme	transmembrane	1271:1283	arg1	protein-3					1285:1293	transmembrane protein-3	1271:1293	interferon-induced transmembrane protein-3 (IFITM3) expression	1252:1313	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	10	6	theme	transmembrane	1271:1283	arg1	IFITM3					1296:1301	IFITM3	1296:1301	IFITM3	1296:1301	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	12	7	theme	biogenesis	1877:1886	arg1	inhibitor					1856:1864	a chemical inhibitor	1845:1864	a chemical inhibitor of exosome biogenesis	1845:1886	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	12	8	dep	GW4896	1837:1842	arg1	inhibitor					1856:1864	a chemical inhibitor	1845:1864	a chemical inhibitor of exosome biogenesis	1845:1886	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	1	9	theme	regenerative	199:210	arg1	medicine					212:219	cardiac regenerative medicine	191:219	cardiac regenerative medicine	191:219	Stem cell-based therapies raise hope for cell replacement and provide opportunity for cardiac regenerative medicine and tumor therapy.					
31665090	0	10	link	marrow-derived	33:46	arg1	vesicles					62:69	bone marrow-derived extracellular vesicles	28:69	bone marrow-derived extracellular vesicles	28:69	Radio-detoxified LPS alters bone marrow-derived extracellular vesicles and endothelial progenitor cells.					
31665090	6	11	theme	increased	728:736	arg1	lethality					738:746	an increased lethality	725:746	an increased lethality	725:746	We found an increased lethality after 16 Gy irradiation.					
31665090	2	12	with	system	310:315	arg1	potential					326:334	the potential to improve the therapeutic efficacy of the treatment of a variety of disorders	322:413	the potential to improve the therapeutic efficacy of the treatment of a variety of disorders	322:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	11	13	theme	reduced	1577:1583	arg1	mortality					1606:1614	a reduced radiotherapy-related mortality	1575:1614	a reduced radiotherapy-related mortality	1575:1614	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	14	14	theme	LPS	2119:2121	arg1	treatment					2123:2131	the radio-detoxified LPS treatment	2098:2131	the radio-detoxified LPS treatment	2098:2131	Our data raise the intriguing possibility that IFITM3 may serve as a marker of the radio-detoxified LPS treatment.					
31665090	13	15	theme	in	1937:1938	arg1	results					1945:1951	these in vivo results	1931:1951	these in vivo results	1931:1951	In contrast to these in vivo results, in vitro experiments did not support the effect of sEVs on EPCs.					
31665090	9	16	theme	mass	1080:1083	arg1	analysis					1098:1105	mass spectrometry analysis	1080:1105	mass spectrometry analysis	1080:1105	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	0	17	theme	endothelial	75:85	arg1	cells					98:102	endothelial progenitor cells	75:102	endothelial progenitor cells	75:102	Radio-detoxified LPS alters bone marrow-derived extracellular vesicles and endothelial progenitor cells.					
31665090	2	18	theme	intercellular	287:299	arg1	vesicles					254:261	Extracellular vesicles	240:261	Extracellular vesicles	240:261	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	2	18	theme	intercellular	287:299	arg1	system					310:315	a membrane-enclosed intercellular delivery system	267:315	a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders	267:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	10	19	dep	marrow	1362:1367	arg1	vesicles					1402:1409	cell-derived small extracellular vesicles	1369:1409	bone marrow cell-derived small extracellular vesicles	1357:1409	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	12	20	theme	extracellular	1663:1675	arg1	vesicles					1677:1684	bone marrow-derived extracellular vesicles	1643:1684	bone marrow-derived extracellular vesicles	1643:1684	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	10	21	theme	marrow	1337:1342	arg1	cells					1344:1348	bone marrow cells	1332:1348	bone marrow cells	1332:1348	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	5	22	theme	cardiac	650:656	arg1	injury					658:663	cardiac injury	650:663	cardiac injury	650:663	In a chest-irradiated mouse model of cardiac injury, we investigated the effects of local irradiation.					
31665090	10	23	theme	small	1382:1386	arg1	vesicles					1402:1409	cell-derived small extracellular vesicles	1369:1409	bone marrow cell-derived small extracellular vesicles	1357:1409	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	8	24	theme	RD-LPS	927:932	arg1	administration					909:922	administration	909:922	administration of RD-LPS	909:932	By flow cytometry, we demonstrated that upon administration of RD-LPS, the number of bone marrow-derived endothelial progenitor cells increased in the bone marrow and, in particular, in the circulation.					
31665090	12	25	link	marrow-derived	1648:1661	arg1	vesicles					1677:1684	bone marrow-derived extracellular vesicles	1643:1684	bone marrow-derived extracellular vesicles	1643:1684	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	10	26	theme	bone	1357:1360	arg1	marrow					1362:1367	bone marrow cell-derived small extracellular vesicles	1357:1409	bone marrow cell-derived small extracellular vesicles	1357:1409	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	12	27	theme	progenitor	1783:1792	arg1	cells					1794:1798	endothelial progenitor cells	1771:1798	endothelial progenitor cells	1771:1798	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	9	28	theme	proteomic	1138:1146	arg1	composition					1148:1158	the proteomic composition	1134:1158	the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs)	1134:1222	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	0	29	theme	Radio-detoxified	0:15	arg1	LPS					17:19	Radio-detoxified LPS	0:19	Radio-detoxified LPS	0:19	Radio-detoxified LPS alters bone marrow-derived extracellular vesicles and endothelial progenitor cells.					
31665090	5	30	theme	chest-irradiated	618:633	arg1	model					641:645	a chest-irradiated mouse model	616:645	a chest-irradiated mouse model of cardiac injury	616:663	In a chest-irradiated mouse model of cardiac injury, we investigated the effects of local irradiation.					
31665090	1	31	theme	cell-based	110:119	arg1	therapies					121:129	Stem cell-based therapies	105:129	Stem cell-based therapies	105:129	Stem cell-based therapies raise hope for cell replacement and provide opportunity for cardiac regenerative medicine and tumor therapy.					
31665090	10	32	theme	extracellular	1388:1400	arg1	vesicles					1402:1409	cell-derived small extracellular vesicles	1369:1409	bone marrow cell-derived small extracellular vesicles	1357:1409	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	9	33	theme	marrow	1168:1173	arg1	composition					1148:1158	the proteomic composition	1134:1158	the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs)	1134:1222	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	8	34	theme	marrow-derived	954:967	arg1	cells					992:996	bone marrow-derived endothelial progenitor cells	949:996	bone marrow-derived endothelial progenitor cells	949:996	By flow cytometry, we demonstrated that upon administration of RD-LPS, the number of bone marrow-derived endothelial progenitor cells increased in the bone marrow and, in particular, in the circulation.					
31665090	4	35	theme	mortality	602:610	arg1	risk					559:562	the risk	555:562	the risk of coronary heart disease and cardiac mortality	555:610	Radiotherapy also increases the risk of coronary heart disease and cardiac mortality.					
31665090	10	36	theme	cell-derived	1369:1380	arg1	vesicles					1402:1409	cell-derived small extracellular vesicles	1369:1409	bone marrow cell-derived small extracellular vesicles	1357:1409	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	0	37	theme	marrow-derived	33:46	arg1	vesicles					62:69	bone marrow-derived extracellular vesicles	28:69	bone marrow-derived extracellular vesicles	28:69	Radio-detoxified LPS alters bone marrow-derived extracellular vesicles and endothelial progenitor cells.					
31665090	14	38	theme	intriguing	2038:2047	arg1	possibility					2049:2059	the intriguing possibility that IFITM3 may serve as a marker of the radio-detoxified LPS treatment	2034:2131	the intriguing possibility that IFITM3 may serve as a marker of the radio-detoxified LPS treatment	2034:2131	Our data raise the intriguing possibility that IFITM3 may serve as a marker of the radio-detoxified LPS treatment.					
31665090	8	39	theme	progenitor	981:990	arg1	cells					992:996	bone marrow-derived endothelial progenitor cells	949:996	bone marrow-derived endothelial progenitor cells	949:996	By flow cytometry, we demonstrated that upon administration of RD-LPS, the number of bone marrow-derived endothelial progenitor cells increased in the bone marrow and, in particular, in the circulation.					
31665090	3	40	theme	breast	436:441	arg1	cancer					443:448	breast cancer	436:448	breast cancer	436:448	As the incidence of breast cancer continues to rise, radiotherapy has emerged as a leading treatment modality.					
31665090	14	41	theme	treatment	2123:2131	arg1	marker					2088:2093	a marker	2086:2093	a marker of the radio-detoxified LPS treatment	2086:2131	Our data raise the intriguing possibility that IFITM3 may serve as a marker of the radio-detoxified LPS treatment.					
31665090	14	41	theme	treatment	2123:2131	arg1	IFITM3					2066:2071	IFITM3	2066:2071	IFITM3	2066:2071	Our data raise the intriguing possibility that IFITM3 may serve as a marker of the radio-detoxified LPS treatment.					
31665090	9	42	theme	small	1188:1192	arg1	sEVs					1218:1221	sEVs	1218:1221	sEVs	1218:1221	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	9	42	theme	small	1188:1192	arg1	vesicles					1208:1215	cell-derived small extracellular vesicles	1175:1215	bone marrow cell-derived small extracellular vesicles (sEVs)	1163:1222	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	4	43	theme	heart	576:580	arg1	disease					582:588	coronary heart disease	567:588	coronary heart disease	567:588	Radiotherapy also increases the risk of coronary heart disease and cardiac mortality.					
31665090	14	44	theme	radio-detoxified	2102:2117	arg1	treatment					2123:2131	the radio-detoxified LPS treatment	2098:2131	the radio-detoxified LPS treatment	2098:2131	Our data raise the intriguing possibility that IFITM3 may serve as a marker of the radio-detoxified LPS treatment.					
31665090	2	45	theme	treatment	379:387	arg1	efficacy					363:370	the therapeutic efficacy	347:370	the therapeutic efficacy of the treatment of a variety of disorders	347:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	10	46	theme	interferon-induced	1252:1269	arg1	expression					1304:1313	interferon-induced transmembrane protein-3 (IFITM3) expression	1252:1313	interferon-induced transmembrane protein-3 (IFITM3) expression	1252:1313	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	11	47	theme	LPS	1473:1475	arg1	treatment					1477:1485	radio-detoxified LPS treatment	1456:1485	radio-detoxified LPS treatment	1456:1485	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	7	48	theme	radio-detoxified	786:801	arg1	treatment					816:824	radio-detoxified LPS (RD-LPS) treatment	786:824	radio-detoxified LPS (RD-LPS) treatment	786:824	Importantly, radio-detoxified LPS (RD-LPS) treatment prolonged the survival significantly.					
31665090	12	49	theme	exosome	1869:1875	arg1	biogenesis					1877:1886	exosome biogenesis	1869:1886	exosome biogenesis	1869:1886	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	5	50	theme	local	697:701	arg1	irradiation					703:713	local irradiation	697:713	local irradiation	697:713	In a chest-irradiated mouse model of cardiac injury, we investigated the effects of local irradiation.					
31665090	10	51	theme	protein-3	1285:1293	arg1	expression					1304:1313	interferon-induced transmembrane protein-3 (IFITM3) expression	1252:1313	interferon-induced transmembrane protein-3 (IFITM3) expression	1252:1313	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	1	52	theme	cardiac	191:197	arg1	medicine					212:219	cardiac regenerative medicine	191:219	cardiac regenerative medicine	191:219	Stem cell-based therapies raise hope for cell replacement and provide opportunity for cardiac regenerative medicine and tumor therapy.					
31665090	9	53	link	cell-derived	1175:1186	arg1	sEVs					1218:1221	sEVs	1218:1221	sEVs	1218:1221	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	9	53	link	cell-derived	1175:1186	arg1	vesicles					1208:1215	cell-derived small extracellular vesicles	1175:1215	bone marrow cell-derived small extracellular vesicles (sEVs)	1163:1222	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	10	54	link	cell-derived	1369:1380	arg1	vesicles					1402:1409	cell-derived small extracellular vesicles	1369:1409	bone marrow cell-derived small extracellular vesicles	1357:1409	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	2	55	theme	therapeutic	351:361	arg1	efficacy					363:370	the therapeutic efficacy	347:370	the therapeutic efficacy of the treatment of a variety of disorders	347:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	1	56	theme	tumor	225:229	arg1	therapy					231:237	tumor therapy	225:237	tumor therapy	225:237	Stem cell-based therapies raise hope for cell replacement and provide opportunity for cardiac regenerative medicine and tumor therapy.					
31665090	11	57	theme	radiotherapy-related	1585:1604	arg1	mortality					1606:1614	a reduced radiotherapy-related mortality	1575:1614	a reduced radiotherapy-related mortality	1575:1614	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	2	58	theme	membrane-enclosed	269:285	arg1	vesicles					254:261	Extracellular vesicles	240:261	Extracellular vesicles	240:261	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	2	58	theme	membrane-enclosed	269:285	arg1	system					310:315	a membrane-enclosed intercellular delivery system	267:315	a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders	267:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	11	59	theme	first	1424:1428	arg1	study					1430:1434	the first study	1420:1434	the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality	1420:1614	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	11	59	theme	first	1424:1428	arg1	This					1412:1415	This	1412:1415	This	1412:1415	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	8	60	link	marrow-derived	954:967	arg1	cells					992:996	bone marrow-derived endothelial progenitor cells	949:996	bone marrow-derived endothelial progenitor cells	949:996	By flow cytometry, we demonstrated that upon administration of RD-LPS, the number of bone marrow-derived endothelial progenitor cells increased in the bone marrow and, in particular, in the circulation.					
31665090	12	61	theme	chemical	1847:1854	arg1	inhibitor					1856:1864	a chemical inhibitor	1845:1864	a chemical inhibitor of exosome biogenesis	1845:1886	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	1	62	theme	cell	146:149	arg1	replacement					151:161	cell replacement	146:161	cell replacement	146:161	Stem cell-based therapies raise hope for cell replacement and provide opportunity for cardiac regenerative medicine and tumor therapy.					
31665090	8	63	theme	flow	867:870	arg1	cytometry					872:880	flow cytometry	867:880	flow cytometry	867:880	By flow cytometry, we demonstrated that upon administration of RD-LPS, the number of bone marrow-derived endothelial progenitor cells increased in the bone marrow and, in particular, in the circulation.					
31665090	12	64	theme	marrow-derived	1648:1661	arg1	vesicles					1677:1684	bone marrow-derived extracellular vesicles	1643:1684	bone marrow-derived extracellular vesicles	1643:1684	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	13	65	dep	in	1954:1955	arg1	vitro					1957:1961	vitro	1957:1961	vitro	1957:1961	In contrast to these in vivo results, in vitro experiments did not support the effect of sEVs on EPCs.					
31665090	13	66	theme	sEVs	2005:2008	arg1	effect					1995:2000	the effect	1991:2000	the effect of sEVs on EPCs	1991:2016	In contrast to these in vivo results, in vitro experiments did not support the effect of sEVs on EPCs.					
31665090	3	67	theme	treatment	507:515	arg1	modality					517:524	a leading treatment modality	497:524	a leading treatment modality	497:524	As the incidence of breast cancer continues to rise, radiotherapy has emerged as a leading treatment modality.					
31665090	0	68	theme	progenitor	87:96	arg1	cells					98:102	endothelial progenitor cells	75:102	endothelial progenitor cells	75:102	Radio-detoxified LPS alters bone marrow-derived extracellular vesicles and endothelial progenitor cells.					
31665090	2	69	theme	delivery	301:308	arg1	vesicles					254:261	Extracellular vesicles	240:261	Extracellular vesicles	240:261	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	2	69	theme	delivery	301:308	arg1	system					310:315	a membrane-enclosed intercellular delivery system	267:315	a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders	267:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	12	70	theme	vesicles	1677:1684	arg1	number					1633:1638	the total number	1623:1638	the total number of bone marrow-derived extracellular vesicles	1623:1684	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	9	71	theme	spectrometry	1085:1096	arg1	analysis					1098:1105	mass spectrometry analysis	1080:1105	mass spectrometry analysis	1080:1105	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	12	72	theme	cells	1794:1798	arg1	number					1761:1766	the number	1757:1766	the number of endothelial progenitor cells	1757:1798	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	12	73	theme	total	1627:1631	arg1	number					1633:1638	the total number	1623:1638	the total number of bone marrow-derived extracellular vesicles	1623:1684	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	10	74	theme	bone	1332:1335	arg1	marrow					1337:1342	bone marrow	1332:1342	bone marrow cells	1332:1348	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
31665090	11	75	with	parallel	1561:1568	arg1	mortality					1606:1614	a reduced radiotherapy-related mortality	1575:1614	a reduced radiotherapy-related mortality	1575:1614	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	11	76	theme	progenitor	1534:1543	arg1	EPCs					1552:1555	EPCs	1552:1555	EPCs	1552:1555	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	11	76	theme	progenitor	1534:1543	arg1	cells					1545:1549	circulating endothelial progenitor cells	1510:1549	circulating endothelial progenitor cells (EPCs)	1510:1556	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	6	77	theme	16 Gy	754:758	arg1	irradiation					760:770	16 Gy irradiation	754:770	16 Gy irradiation	754:770	We found an increased lethality after 16 Gy irradiation.					
31665090	5	78	theme	injury	658:663	arg1	model					641:645	a chest-irradiated mouse model	616:645	a chest-irradiated mouse model of cardiac injury	616:663	In a chest-irradiated mouse model of cardiac injury, we investigated the effects of local irradiation.					
31665090	11	79	theme	circulating	1510:1520	arg1	EPCs					1552:1555	EPCs	1552:1555	EPCs	1552:1555	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	11	79	theme	circulating	1510:1520	arg1	cells					1545:1549	circulating endothelial progenitor cells	1510:1549	circulating endothelial progenitor cells (EPCs)	1510:1556	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	12	80	theme	endothelial	1771:1781	arg1	cells					1794:1798	endothelial progenitor cells	1771:1798	endothelial progenitor cells	1771:1798	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	4	81	theme	cardiac	594:600	arg1	mortality					602:610	cardiac mortality	594:610	cardiac mortality	594:610	Radiotherapy also increases the risk of coronary heart disease and cardiac mortality.					
31665090	5	82	theme	mouse	635:639	arg1	model					641:645	a chest-irradiated mouse model	616:645	a chest-irradiated mouse model of cardiac injury	616:663	In a chest-irradiated mouse model of cardiac injury, we investigated the effects of local irradiation.					
31665090	13	83	from	effect	1995:2000	arg1	EPCs					2013:2016	EPCs	2013:2016	EPCs	2013:2016	In contrast to these in vivo results, in vitro experiments did not support the effect of sEVs on EPCs.					
31665090	12	84	theme	RD-LPS	1742:1747	arg1	groups					1749:1754	the RD-LPS groups	1738:1754	the RD-LPS groups	1738:1754	While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls.					
31665090	13	85	dep	results	1945:1951	arg1	contrast					1919:1926	contrast	1919:1926	contrast	1919:1926	In contrast to these in vivo results, in vitro experiments did not support the effect of sEVs on EPCs.					
31665090	9	86	theme	bone	1163:1166	arg1	marrow					1168:1173	bone marrow cell-derived small extracellular vesicles (sEVs)	1163:1222	bone marrow cell-derived small extracellular vesicles (sEVs)	1163:1222	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	3	87	theme	leading	499:505	arg1	modality					517:524	a leading treatment modality	497:524	a leading treatment modality	497:524	As the incidence of breast cancer continues to rise, radiotherapy has emerged as a leading treatment modality.					
31665090	0	88	theme	extracellular	48:60	arg1	vesicles					62:69	bone marrow-derived extracellular vesicles	28:69	bone marrow-derived extracellular vesicles	28:69	Radio-detoxified LPS alters bone marrow-derived extracellular vesicles and endothelial progenitor cells.					
31665090	11	89	theme	cells	1545:1549	arg1	increase					1498:1505	an increase	1495:1505	an increase of circulating endothelial progenitor cells (EPCs)	1495:1556	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	4	90	theme	coronary	567:574	arg1	disease					582:588	coronary heart disease	567:588	coronary heart disease	567:588	Radiotherapy also increases the risk of coronary heart disease and cardiac mortality.					
31665090	9	91	theme	cell-derived	1175:1186	arg1	sEVs					1218:1221	sEVs	1218:1221	sEVs	1218:1221	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	9	91	theme	cell-derived	1175:1186	arg1	vesicles					1208:1215	cell-derived small extracellular vesicles	1175:1215	bone marrow cell-derived small extracellular vesicles (sEVs)	1163:1222	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	8	92	theme	endothelial	969:979	arg1	cells					992:996	bone marrow-derived endothelial progenitor cells	949:996	bone marrow-derived endothelial progenitor cells	949:996	By flow cytometry, we demonstrated that upon administration of RD-LPS, the number of bone marrow-derived endothelial progenitor cells increased in the bone marrow and, in particular, in the circulation.					
31665090	3	93	theme	cancer	443:448	arg1	incidence					423:431	the incidence	419:431	the incidence of breast cancer	419:448	As the incidence of breast cancer continues to rise, radiotherapy has emerged as a leading treatment modality.					
31665090	4	94	theme	disease	582:588	arg1	risk					559:562	the risk	555:562	the risk of coronary heart disease and cardiac mortality	555:610	Radiotherapy also increases the risk of coronary heart disease and cardiac mortality.					
31665090	11	95	theme	endothelial	1522:1532	arg1	EPCs					1552:1555	EPCs	1552:1555	EPCs	1552:1555	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	11	95	theme	endothelial	1522:1532	arg1	cells					1545:1549	circulating endothelial progenitor cells	1510:1549	circulating endothelial progenitor cells (EPCs)	1510:1556	This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality.					
31665090	2	96	theme	Extracellular	240:252	arg1	vesicles					254:261	Extracellular vesicles	240:261	Extracellular vesicles	240:261	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	2	96	theme	Extracellular	240:252	arg1	system					310:315	a membrane-enclosed intercellular delivery system	267:315	a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders	267:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	9	97	theme	extracellular	1194:1206	arg1	sEVs					1218:1221	sEVs	1218:1221	sEVs	1218:1221	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	9	97	theme	extracellular	1194:1206	arg1	vesicles					1208:1215	cell-derived small extracellular vesicles	1175:1215	bone marrow cell-derived small extracellular vesicles (sEVs)	1163:1222	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	8	98	theme	cells	992:996	arg1	number					939:944	the number	935:944	the number of bone marrow-derived endothelial progenitor cells	935:996	By flow cytometry, we demonstrated that upon administration of RD-LPS, the number of bone marrow-derived endothelial progenitor cells increased in the bone marrow and, in particular, in the circulation.					
31665090	7	99	theme	RD-LPS	808:813	arg1	treatment					816:824	radio-detoxified LPS (RD-LPS) treatment	786:824	radio-detoxified LPS (RD-LPS) treatment	786:824	Importantly, radio-detoxified LPS (RD-LPS) treatment prolonged the survival significantly.					
31665090	9	100	dep	marrow	1168:1173	arg1	sEVs					1218:1221	sEVs	1218:1221	sEVs	1218:1221	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	9	100	dep	marrow	1168:1173	arg1	vesicles					1208:1215	cell-derived small extracellular vesicles	1175:1215	bone marrow cell-derived small extracellular vesicles (sEVs)	1163:1222	Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs).					
31665090	7	101	theme	LPS	803:805	arg1	treatment					816:824	radio-detoxified LPS (RD-LPS) treatment	786:824	radio-detoxified LPS (RD-LPS) treatment	786:824	Importantly, radio-detoxified LPS (RD-LPS) treatment prolonged the survival significantly.					
31665090	2	102	theme	disorders	405:413	arg1	disorders					405:413	disorders	405:413	disorders	405:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	2	102	theme	disorders	405:413	arg1	variety					394:400	a variety	392:400	a variety of disorders	392:413	Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders.					
31665090	10	103	theme	RD-LPS	1225:1230	arg1	treatment					1232:1240	RD-LPS treatment	1225:1240	RD-LPS treatment	1225:1240	RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles.					
30261158	0	0	theme	fibroblasts	63:73	arg1	Culture					0:6	Culture	0:6	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.	0:98	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.					
30261158	5	1	theme	dimentional	1003:1013	arg1	culture					1015:1021	a three dimentional culture	995:1021	a three dimentional culture	995:1021	However, PNPs showed difficulty in attaching to and growing neurites on most of the materials tested, except for matrigel, in which neurite growth was observed in a three dimentional culture.					
30261158	4	2	contain	have	782:785	arg2	potentials					787:796	potentials	787:796	potentials in neural circuit reconstruction	787:829	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	4	2	contain	have	782:785	arg1	neurons					739:745	Pyramidal neurons	729:745	Pyramidal neurons	729:745	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	9	3	theme	many	1415:1418	arg1	indications					1420:1430	many indications	1415:1430	many indications	1415:1430	Fibroblast graft are useful in many indications, such as in skin burns.					
30261158	9	3	theme	many	1415:1418	arg1	burns					1449:1453	skin burns	1444:1453	skin burns	1444:1453	Fibroblast graft are useful in many indications, such as in skin burns.					
30261158	3	4	theme	lactic-co-glycolic	602:619	arg1	collagen-poly					587:599	an aligned collagen-poly	576:599	an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite	576:642	Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only.					
30261158	3	4	theme	lactic-co-glycolic	602:619	arg1	acid					621:624	lactic-co-glycolic acid	602:624	lactic-co-glycolic acid	602:624	Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only.					
30261158	4	5	theme	projection	759:768	arg1	ability					770:776	strong projection ability	752:776	strong projection ability	752:776	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	9	6	from	indications	1420:1430	arg1	useful					1405:1410	useful	1405:1410	useful	1405:1410	Fibroblast graft are useful in many indications, such as in skin burns.					
30261158	2	7	theme	stem	327:330	arg1	cells					332:336	neural stem cells	320:336	neural stem cells (NSCs)	320:343	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	2	7	theme	stem	327:330	arg1	NSCs					339:342	NSCs	339:342	NSCs	339:342	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	0	8	theme	various	78:84	arg1	biomaterials					86:97	various biomaterials	78:97	various biomaterials	78:97	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.					
30261158	0	9	from	Culture	0:6	arg1	biomaterials					86:97	various biomaterials	78:97	various biomaterials	78:97	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.					
30261158	6	10	theme	neurological	1070:1081	arg1	diseases					1083:1090	neurological diseases	1070:1090	neurological diseases	1070:1090	NSCs and derivatives hold promise in treating neurological diseases.					
30261158	8	11	theme	stem	1258:1261	arg1	iNSCs					1270:1274	iNSCs	1270:1274	iNSCs	1270:1274	On aligned collagen-PLGA, induced neural stem cells (iNSCs) could survive and differentiate for at least 2 weeks, but the neurites failed to extend in an aligned way.					
30261158	8	11	theme	stem	1258:1261	arg1	cells					1263:1267	neural stem cells	1251:1267	induced neural stem cells (iNSCs)	1243:1275	On aligned collagen-PLGA, induced neural stem cells (iNSCs) could survive and differentiate for at least 2 weeks, but the neurites failed to extend in an aligned way.					
30261158	3	12	theme	aligned	710:716	arg1	form					718:721	an aligned form	707:721	an aligned form only	707:726	Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only.					
30261158	7	13	theme	aligned	1096:1102	arg1	fibrin					1104:1109	aligned fibrin	1096:1109	aligned fibrin	1096:1109	On aligned fibrin, NSCs could differentiate and grow neurites in a directional manner before fibrin was degraded in 2 days.					
30261158	8	14	theme	aligned	1371:1377	arg1	way					1379:1381	an aligned way	1368:1381	an aligned way	1368:1381	On aligned collagen-PLGA, induced neural stem cells (iNSCs) could survive and differentiate for at least 2 weeks, but the neurites failed to extend in an aligned way.					
30261158	4	15	from	potentials	787:796	arg1	reconstruction					816:829	neural circuit reconstruction	801:829	neural circuit reconstruction	801:829	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	7	16	theme	directional	1160:1170	arg1	manner					1172:1177	a directional manner	1158:1177	a directional manner	1158:1177	On aligned fibrin, NSCs could differentiate and grow neurites in a directional manner before fibrin was degraded in 2 days.					
30261158	9	17	from	useful	1405:1410	arg1	indications					1420:1430	many indications	1415:1430	many indications	1415:1430	Fibroblast graft are useful in many indications, such as in skin burns.					
30261158	9	17	from	useful	1405:1410	arg1	burns					1449:1453	skin burns	1444:1453	skin burns	1444:1453	Fibroblast graft are useful in many indications, such as in skin burns.					
30261158	4	18	theme	Pyramidal	729:737	arg1	neurons					739:745	Pyramidal neurons	729:745	Pyramidal neurons	729:745	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	5	19	from	neurites	892:899	arg1	most					904:907	most	904:907	most	904:907	However, PNPs showed difficulty in attaching to and growing neurites on most of the materials tested, except for matrigel, in which neurite growth was observed in a three dimentional culture.					
30261158	5	19	from	neurites	892:899	arg1	materials					916:924	the materials	912:924	the materials tested	912:931	However, PNPs showed difficulty in attaching to and growing neurites on most of the materials tested, except for matrigel, in which neurite growth was observed in a three dimentional culture.					
30261158	10	20	theme	aligned	1589:1595	arg1	fibrin					1597:1602	the aligned fibrin	1585:1602	the aligned fibrin	1585:1602	Fibroblasts generally grew better on the tested materials than did the neural cells, and fibroblasts could grow directionally on the aligned fibrin and scattered around on the alveolate collagen.					
30261158	2	21	theme	type	538:541	arg1	culture					517:523	culture	517:523	culture of each cell type	517:541	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	11	22	used	used	1696:1699	arg2	information					1671:1681	information	1671:1681	information which may be used to further optimize the materials to support culture of each type of cells	1671:1774	The study provided information which may be used to further optimize the materials to support culture of each type of cells.					
30261158	8	23	theme	neural	1251:1256	arg1	iNSCs					1270:1274	iNSCs	1270:1274	iNSCs	1270:1274	On aligned collagen-PLGA, induced neural stem cells (iNSCs) could survive and differentiate for at least 2 weeks, but the neurites failed to extend in an aligned way.					
30261158	8	23	theme	neural	1251:1256	arg1	cells					1263:1267	neural stem cells	1251:1267	induced neural stem cells (iNSCs)	1243:1275	On aligned collagen-PLGA, induced neural stem cells (iNSCs) could survive and differentiate for at least 2 weeks, but the neurites failed to extend in an aligned way.					
30261158	2	24	theme	cell	533:536	arg1	type					538:541	each cell type	528:541	each cell type	528:541	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	8	25	theme	induced	1243:1249	arg1	iNSCs					1270:1274	iNSCs	1270:1274	iNSCs	1270:1274	On aligned collagen-PLGA, induced neural stem cells (iNSCs) could survive and differentiate for at least 2 weeks, but the neurites failed to extend in an aligned way.					
30261158	8	25	theme	induced	1243:1249	arg1	cells					1263:1267	neural stem cells	1251:1267	induced neural stem cells (iNSCs)	1243:1275	On aligned collagen-PLGA, induced neural stem cells (iNSCs) could survive and differentiate for at least 2 weeks, but the neurites failed to extend in an aligned way.					
30261158	0	26	theme	neural	21:26	arg1	precursors					28:37	pyramidal neural precursors	11:37	pyramidal neural precursors	11:37	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.					
30261158	11	27	theme	cells	1770:1774	arg1	type					1762:1765	each type	1757:1765	each type of cells	1757:1774	The study provided information which may be used to further optimize the materials to support culture of each type of cells.					
30261158	2	28	theme	neural	294:299	arg1	PNPs					313:316	PNPs	313:316	PNPs	313:316	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	2	28	theme	neural	294:299	arg1	precursors					301:310	pyramidal neural precursors	284:310	pyramidal neural precursors (PNPs)	284:317	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	0	29	theme	pyramidal	11:19	arg1	precursors					28:37	pyramidal neural precursors	11:37	pyramidal neural precursors	11:37	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.					
30261158	2	30	theme	pyramidal	284:292	arg1	PNPs					313:316	PNPs	313:316	PNPs	313:316	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	2	30	theme	pyramidal	284:292	arg1	precursors					301:310	pyramidal neural precursors	284:310	pyramidal neural precursors (PNPs)	284:317	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	2	31	theme	hyaluronic	417:426	arg1	HA					434:435	HA	434:435	HA	434:435	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	2	31	theme	hyaluronic	417:426	arg1	acid					428:431	hyaluronic acid	417:431	hyaluronic acid (HA)	417:436	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	1	32	theme	cell	190:193	arg1	therapy					195:201	cell therapy	190:201	cell therapy	190:201	A combinatory approach using biomaterials together with cells may improve the efficacy of cell therapy for treatment of various diseases/indications.					
30261158	3	33	theme	alveolate	651:659	arg1	form					661:664	an alveolate form	648:664	an alveolate form	648:664	Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only.					
30261158	1	34	theme	therapy	195:201	arg1	efficacy					178:185	the efficacy	174:185	the efficacy of cell therapy for treatment of various diseases/indications	174:247	A combinatory approach using biomaterials together with cells may improve the efficacy of cell therapy for treatment of various diseases/indications.					
30261158	10	35	dep	materials	1504:1512	arg1	did					1519:1521	did	1519:1521	did	1519:1521	Fibroblasts generally grew better on the tested materials than did the neural cells, and fibroblasts could grow directionally on the aligned fibrin and scattered around on the alveolate collagen.					
30261158	2	36	theme	different	365:373	arg1	materials					375:383	different materials	365:383	different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel	365:466	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	0	37	theme	precursors	28:37	arg1	Culture					0:6	Culture	0:6	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.	0:98	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.					
30261158	3	38	theme	hyaluronic	678:687	arg1	acid					689:692	hyaluronic acid	678:692	hyaluronic acid	678:692	Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only.					
30261158	5	39	theme	neurite	964:970	arg1	growth					972:977	neurite growth	964:977	neurite growth	964:977	However, PNPs showed difficulty in attaching to and growing neurites on most of the materials tested, except for matrigel, in which neurite growth was observed in a three dimentional culture.					
30261158	10	40	theme	alveolate	1632:1640	arg1	collagen					1642:1649	the alveolate collagen	1628:1649	the alveolate collagen	1628:1649	Fibroblasts generally grew better on the tested materials than did the neural cells, and fibroblasts could grow directionally on the aligned fibrin and scattered around on the alveolate collagen.					
30261158	10	41	theme	tested	1497:1502	arg1	materials					1504:1512	the tested materials	1493:1512	the tested materials than did the neural cells	1493:1538	Fibroblasts generally grew better on the tested materials than did the neural cells, and fibroblasts could grow directionally on the aligned fibrin and scattered around on the alveolate collagen.					
30261158	0	42	theme	stem	47:50	arg1	cells					52:56	neural stem cells	40:56	neural stem cells	40:56	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.					
30261158	10	43	dep	did	1519:1521	arg1	than					1514:1517	than	1514:1517	than	1514:1517	Fibroblasts generally grew better on the tested materials than did the neural cells, and fibroblasts could grow directionally on the aligned fibrin and scattered around on the alveolate collagen.					
30261158	2	44	theme	neural	320:325	arg1	cells					332:336	neural stem cells	320:336	neural stem cells (NSCs)	320:343	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	2	44	theme	neural	320:325	arg1	NSCs					339:342	NSCs	339:342	NSCs	339:342	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	0	45	theme	neural	40:45	arg1	cells					52:56	neural stem cells	40:56	neural stem cells	40:56	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.					
30261158	2	46	theme	suitable	492:499	arg1	biomaterial					501:511	the most suitable biomaterial	483:511	the most suitable biomaterial	483:511	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	3	47	theme	collagen-poly	587:599	arg1	composite					634:642	an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite	576:642	an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite	576:642	Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only.					
30261158	9	48	theme	skin	1444:1447	arg1	burns					1449:1453	skin burns	1444:1453	skin burns	1444:1453	Fibroblast graft are useful in many indications, such as in skin burns.					
30261158	1	49	theme	combinatory	102:112	arg1	approach					114:121	A combinatory approach	100:121	A combinatory approach using biomaterials together with cells	100:160	A combinatory approach using biomaterials together with cells may improve the efficacy of cell therapy for treatment of various diseases/indications.					
30261158	4	50	contain	have	747:750	arg2	ability					770:776	strong projection ability	752:776	strong projection ability	752:776	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	4	50	contain	have	747:750	arg1	neurons					739:745	Pyramidal neurons	729:745	Pyramidal neurons	729:745	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	4	51	theme	circuit	808:814	arg1	reconstruction					816:829	neural circuit reconstruction	801:829	neural circuit reconstruction	801:829	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	2	52	theme	current	257:263	arg1	study					265:269	the current study	253:269	the current study	253:269	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	10	53	theme	neural	1527:1532	arg1	cells					1534:1538	the neural cells	1523:1538	the neural cells	1523:1538	Fibroblasts generally grew better on the tested materials than did the neural cells, and fibroblasts could grow directionally on the aligned fibrin and scattered around on the alveolate collagen.					
30261158	9	54	theme	Fibroblast	1384:1393	arg1	graft					1395:1399	Fibroblast graft	1384:1399	Fibroblast graft	1384:1399	Fibroblast graft are useful in many indications, such as in skin burns.					
30261158	4	55	theme	neural	801:806	arg1	reconstruction					816:829	neural circuit reconstruction	801:829	neural circuit reconstruction	801:829	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	4	56	theme	strong	752:757	arg1	ability					770:776	strong projection ability	752:776	strong projection ability	752:776	Pyramidal neurons have strong projection ability and have potentials in neural circuit reconstruction.					
30261158	8	57	theme	aligned	1220:1226	arg1	collagen-PLGA					1228:1240	aligned collagen-PLGA	1220:1240	aligned collagen-PLGA	1220:1240	On aligned collagen-PLGA, induced neural stem cells (iNSCs) could survive and differentiate for at least 2 weeks, but the neurites failed to extend in an aligned way.					
30261158	2	58	theme	sciatic	439:445	arg1	nerves					447:452	sciatic nerves	439:452	sciatic nerves	439:452	In the current study, we cultured pyramidal neural precursors (PNPs), neural stem cells (NSCs), and fibroblasts on different materials that included fibrin, collagen, hyaluronic acid (HA), sciatic nerves, and matrigel, to search for the most suitable biomaterial for culture of each cell type.					
30261158	3	59	theme	aligned	579:585	arg1	collagen-poly					587:599	an aligned collagen-poly	576:599	an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite	576:642	Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only.					
30261158	3	59	theme	aligned	579:585	arg1	acid					621:624	lactic-co-glycolic acid	602:624	lactic-co-glycolic acid	602:624	Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only.					
30261158	3	59	theme	aligned	579:585	arg1	PLGA					628:631	PLGA	628:631	PLGA	628:631	Collagen was fabricated in both an aligned collagen-poly (lactic-co-glycolic acid) (PLGA) composite and an alveolate form; fibrin and hyaluronic acid were made in an aligned form only.					
30261158	5	60	located	observed	983:990	arg2	growth					972:977	neurite growth	964:977	neurite growth	964:977	However, PNPs showed difficulty in attaching to and growing neurites on most of the materials tested, except for matrigel, in which neurite growth was observed in a three dimentional culture.					
30261158	5	60	located	observed	983:990	arg1	matrigel					945:952	matrigel	945:952	matrigel	945:952	However, PNPs showed difficulty in attaching to and growing neurites on most of the materials tested, except for matrigel, in which neurite growth was observed in a three dimentional culture.					
30261158	5	60	located	observed	983:990	arg1	culture					1015:1021	a three dimentional culture	995:1021	a three dimentional culture	995:1021	However, PNPs showed difficulty in attaching to and growing neurites on most of the materials tested, except for matrigel, in which neurite growth was observed in a three dimentional culture.					
30261158	1	61	theme	various	220:226	arg1	diseases/indications					228:247	various diseases/indications	220:247	various diseases/indications	220:247	A combinatory approach using biomaterials together with cells may improve the efficacy of cell therapy for treatment of various diseases/indications.					
30261158	0	62	theme	cells	52:56	arg1	Culture					0:6	Culture	0:6	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.	0:98	Culture of pyramidal neural precursors, neural stem cells, and fibroblasts on various biomaterials.					
30261158	11	63	theme	type	1762:1765	arg1	culture					1746:1752	culture	1746:1752	culture of each type of cells	1746:1774	The study provided information which may be used to further optimize the materials to support culture of each type of cells.					
30261158	1	64	theme	diseases/indications	228:247	arg1	treatment					207:215	treatment	207:215	treatment of various diseases/indications	207:247	A combinatory approach using biomaterials together with cells may improve the efficacy of cell therapy for treatment of various diseases/indications.					
31684724	5	0	theme	2.52	1149:1152	arg1	GF					1142:1143	GF	1142:1143	GF	1142:1143	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	5	0	theme	2.52	1149:1152	arg1	factor					1134:1139	a gauge factor	1126:1139	a gauge factor (GF)	1126:1144	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	5	0	theme	2.52	1149:1152	arg1	larger					1176:1181	larger	1176:1181	larger	1176:1181	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	3	1	theme	high	732:735	arg1	toughness					737:745	high toughness	732:745	high toughness	732:745	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	2	dep	strength	683:690	arg1	increase					698:705	409% increase	693:705	409% increase compared to pure PVA and high toughness up to 407.1%	693:758	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	1	3	theme	robotic	180:186	arg1	prosthetics					188:198	robotic prosthetics	180:198	robotic prosthetics	180:198	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	4	4	theme	min	1006:1008	arg1	time					995:998	a healing time	985:998	a healing time of 30 min	985:1008	When damaged, the nanocomposite films displayed good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery after a healing time of 30 min.					
31684724	5	5	theme	subtle	1097:1102	arg1	strains					1104:1110	subtle strains	1097:1110	subtle strains (<48.5%)	1097:1119	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	5	5	theme	subtle	1097:1102	arg1	%					1118:1118	<48.5%	1113:1118	<48.5%	1113:1118	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	3	6	theme	pure	719:722	arg1	PVA					724:726	pure PVA	719:726	pure PVA	719:726	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	7	theme	weak	531:534	arg1	H-bonds					536:542	weak H-bonds	531:542	weak H-bonds	531:542	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	6	8	theme	large	1326:1330	arg1	motions					1349:1355	large and subtle human motions	1326:1355	large and subtle human motions (finger bending motions, swallowing, and wrist pulse)	1326:1409	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	3	9	theme	various	828:834	arg1	substrates					836:845	various substrates	828:845	various substrates	828:845	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	5	10	theme	gauge	1128:1132	arg1	GF					1142:1143	GF	1142:1143	GF	1142:1143	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	5	10	theme	gauge	1128:1132	arg1	factor					1134:1139	a gauge factor	1126:1139	a gauge factor (GF)	1126:1144	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	5	10	theme	gauge	1128:1132	arg1	larger					1176:1181	larger	1176:1181	larger	1176:1181	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	3	11	theme	bonds	566:570	arg1	combination					516:526	The combination	512:526	The combination of weak H-bonds and iron coordination bonds	512:570	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	11	theme	bonds	566:570	arg1	effect					592:597	the synergistic effect	576:597	the synergistic effect of these components	576:617	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	12	theme	synergistic	580:590	arg1	effect					592:597	the synergistic effect	576:597	the synergistic effect of these components	576:617	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	4	13	dep	mechanical	918:927	arg1	%					939:939	72.0-76.3%	930:939	72.0-76.3%	930:939	When damaged, the nanocomposite films displayed good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery after a healing time of 30 min.					
31684724	3	14	dep	adhesion	775:782	arg1	times					790:794	9670 times	785:794	9670 times greater than its own weight	785:822	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	6	15	theme	health	1448:1453	arg1	monitoring					1455:1464	health monitoring	1448:1464	health monitoring	1448:1464	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	2	16	theme	iron	422:425	arg1	oxidant					449:455	iron(III) chloride (FeCl3) oxidant	422:455	iron(III) chloride (FeCl3) oxidant	422:455	Polypyrrole-coated cellulose nanocrystals and cellulose nanofibers were prepared using iron(III) chloride (FeCl3) oxidant, which were used to reinforce polyvinyl alcohol (PVA).					
31684724	2	17	theme	cellulose	381:389	arg1	nanofibers					391:400	cellulose nanofibers	381:400	cellulose nanofibers	381:400	Polypyrrole-coated cellulose nanocrystals and cellulose nanofibers were prepared using iron(III) chloride (FeCl3) oxidant, which were used to reinforce polyvinyl alcohol (PVA).					
31684724	2	18	used	used	469:472	arg2	oxidant					449:455	iron(III) chloride (FeCl3) oxidant	422:455	iron(III) chloride (FeCl3) oxidant	422:455	Polypyrrole-coated cellulose nanocrystals and cellulose nanofibers were prepared using iron(III) chloride (FeCl3) oxidant, which were used to reinforce polyvinyl alcohol (PVA).					
31684724	6	19	theme	wrist	1398:1402	arg1	pulse					1404:1408	wrist pulse	1398:1408	wrist pulse	1398:1408	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	3	20	theme	iron	548:551	arg1	bonds					566:570	iron coordination bonds	548:570	iron coordination bonds	548:570	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	1	21	theme	human-machine	205:217	arg1	interactions					219:230	human-machine interactions	205:230	human-machine interactions	205:230	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	2	22	theme	FeCl3	442:446	arg1	oxidant					449:455	iron(III) chloride (FeCl3) oxidant	422:455	iron(III) chloride (FeCl3) oxidant	422:455	Polypyrrole-coated cellulose nanocrystals and cellulose nanofibers were prepared using iron(III) chloride (FeCl3) oxidant, which were used to reinforce polyvinyl alcohol (PVA).					
31684724	0	23	theme	Electronic	72:81	arg1	Systems					95:101	Biocompatible Electronic Skin Sensor Systems	58:101	Biocompatible Electronic Skin Sensor Systems	58:101	Self-Healable Conductive Nanocellulose Nanocomposites for Biocompatible Electronic Skin Sensor Systems.					
31684724	5	24	theme	strain	1072:1077	arg1	sensitivity					1079:1089	high strain sensitivity	1067:1089	high strain sensitivity under subtle strains (<48.5%)	1067:1119	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	6	25	contain	have	1423:1426	arg2	sensors					1487:1493	flexible skin sensors	1473:1493	flexible skin sensors	1473:1493	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	25	contain	have	1423:1426	arg1	they					1418:1421	they	1418:1421	they	1418:1421	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	25	contain	have	1423:1426	arg2	potentials					1434:1443	great potentials	1428:1443	great potentials	1428:1443	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	1	26	theme	Electronic	104:113	arg1	skins					115:119	Electronic skins	104:119	Electronic skins	104:119	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	6	27	theme	finger	1358:1363	arg1	motions					1373:1379	finger bending motions	1358:1379	finger bending motions	1358:1379	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	4	28	theme	conductive	946:955	arg1	recovery					970:977	good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery	913:977	good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery	913:977	When damaged, the nanocomposite films displayed good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery after a healing time of 30 min.					
31684724	0	29	theme	Conductive	14:23	arg1	Nanocellulose					25:37	Self-Healable Conductive Nanocellulose	0:37	Self-Healable Conductive Nanocellulose	0:37	Self-Healable Conductive Nanocellulose Nanocomposites for Biocompatible Electronic Skin Sensor Systems.					
31684724	4	30	theme	nanocomposite	883:895	arg1	films					897:901	the nanocomposite films	879:901	the nanocomposite films	879:901	When damaged, the nanocomposite films displayed good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery after a healing time of 30 min.					
31684724	5	31	theme	high	1067:1070	arg1	sensitivity					1079:1089	high strain sensitivity	1067:1089	high strain sensitivity under subtle strains (<48.5%)	1067:1119	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	2	32	theme	chloride	432:439	arg1	oxidant					449:455	iron(III) chloride (FeCl3) oxidant	422:455	iron(III) chloride (FeCl3) oxidant	422:455	Polypyrrole-coated cellulose nanocrystals and cellulose nanofibers were prepared using iron(III) chloride (FeCl3) oxidant, which were used to reinforce polyvinyl alcohol (PVA).					
31684724	0	33	theme	Self-Healable	0:12	arg1	Nanocellulose					25:37	Self-Healable Conductive Nanocellulose	0:37	Self-Healable Conductive Nanocellulose	0:37	Self-Healable Conductive Nanocellulose Nanocomposites for Biocompatible Electronic Skin Sensor Systems.					
31684724	4	34	theme	healing	987:993	arg1	time					995:998	a healing time	985:998	a healing time of 30 min	985:1008	When damaged, the nanocomposite films displayed good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery after a healing time of 30 min.					
31684724	5	35	theme	sensors	1224:1230	arg1	GF					1192:1193	the GF	1188:1193	the GF of ionic hydrogel-based skin sensors	1188:1230	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	1	36	theme	sensing	315:321	arg1	properties					323:332	sensing properties	315:332	sensing properties	315:332	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	4	37	theme	good	913:916	arg1	recovery					970:977	good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery	913:977	good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery	913:977	When damaged, the nanocomposite films displayed good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery after a healing time of 30 min.					
31684724	2	38	theme	cellulose	354:362	arg1	nanocrystals					364:375	Polypyrrole-coated cellulose nanocrystals	335:375	Polypyrrole-coated cellulose nanocrystals	335:375	Polypyrrole-coated cellulose nanocrystals and cellulose nanofibers were prepared using iron(III) chloride (FeCl3) oxidant, which were used to reinforce polyvinyl alcohol (PVA).					
31684724	6	39	theme	great	1428:1432	arg1	potentials					1434:1443	great potentials	1428:1443	great potentials	1428:1443	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	40	contain	possessed	1259:1267	arg2	performance					1286:1296	superior sensing performance	1269:1296	superior sensing performance	1269:1296	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	40	contain	possessed	1259:1267	arg1	films					1253:1257	These nanocomposite films	1233:1257	These nanocomposite films	1233:1257	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	41	dep	motions	1349:1355	arg1	motions					1373:1379	finger bending motions	1358:1379	finger bending motions	1358:1379	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	41	dep	motions	1349:1355	arg1	pulse					1404:1408	wrist pulse	1398:1408	wrist pulse	1398:1408	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	41	dep	motions	1349:1355	arg1	swallowing					1382:1391	swallowing	1382:1391	swallowing	1382:1391	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	42	theme	human	1343:1347	arg1	motions					1349:1355	large and subtle human motions	1326:1355	large and subtle human motions (finger bending motions, swallowing, and wrist pulse)	1326:1409	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	4	43	theme	mechanical	918:927	arg1	recovery					970:977	good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery	913:977	good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery	913:977	When damaged, the nanocomposite films displayed good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery after a healing time of 30 min.					
31684724	2	44	theme	Polypyrrole-coated	335:352	arg1	nanocrystals					364:375	Polypyrrole-coated cellulose nanocrystals	335:375	Polypyrrole-coated cellulose nanocrystals	335:375	Polypyrrole-coated cellulose nanocrystals and cellulose nanofibers were prepared using iron(III) chloride (FeCl3) oxidant, which were used to reinforce polyvinyl alcohol (PVA).					
31684724	0	45	theme	Biocompatible	58:70	arg1	Systems					95:101	Biocompatible Electronic Skin Sensor Systems	58:101	Biocompatible Electronic Skin Sensor Systems	58:101	Self-Healable Conductive Nanocellulose Nanocomposites for Biocompatible Electronic Skin Sensor Systems.					
31684724	6	46	theme	superior	1269:1276	arg1	performance					1286:1296	superior sensing performance	1269:1296	superior sensing performance	1269:1296	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	3	47	theme	409	693:695	arg1	%					696:696	%	696:696	%	696:696	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	48	dep	increase	698:705	arg1	%					758:758	up to 407.1%	747:758	409% increase compared to pure PVA and high toughness up to 407.1%	693:758	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	49	theme	mechanical	672:681	arg1	strength					683:690	robust mechanical strength	665:690	robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%)	665:759	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	50	theme	greater	796:802	arg1	times					790:794	9670 times	785:794	9670 times greater than its own weight	785:822	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	5	51	contain	possessed	1057:1065	arg2	sensitivity					1079:1089	high strain sensitivity	1067:1089	high strain sensitivity under subtle strains (<48.5%)	1067:1119	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	5	51	contain	possessed	1057:1065	arg1	nanocomposites					1042:1055	the flexible nanocomposites	1029:1055	the flexible nanocomposites	1029:1055	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	0	52	theme	Skin	83:86	arg1	Systems					95:101	Biocompatible Electronic Skin Sensor Systems	58:101	Biocompatible Electronic Skin Sensor Systems	58:101	Self-Healable Conductive Nanocellulose Nanocomposites for Biocompatible Electronic Skin Sensor Systems.					
31684724	7	53	theme	electronic	1509:1518	arg1	devices					1520:1526	wearable electronic devices	1500:1526	wearable electronic devices	1500:1526	and wearable electronic devices.					
31684724	6	54	theme	nanocomposite	1239:1251	arg1	films					1253:1257	These nanocomposite films	1233:1257	These nanocomposite films	1233:1257	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	3	55	theme	excellent	765:773	arg1	adhesion					775:782	excellent adhesion	765:782	excellent adhesion (9670 times greater than its own weight) to various substrates	765:845	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	2	56	theme	polyvinyl	487:495	arg1	alcohol					497:503	polyvinyl alcohol	487:503	polyvinyl alcohol (PVA)	487:509	Polypyrrole-coated cellulose nanocrystals and cellulose nanofibers were prepared using iron(III) chloride (FeCl3) oxidant, which were used to reinforce polyvinyl alcohol (PVA).					
31684724	2	56	theme	polyvinyl	487:495	arg1	PVA					506:508	PVA	506:508	PVA	506:508	Polypyrrole-coated cellulose nanocrystals and cellulose nanofibers were prepared using iron(III) chloride (FeCl3) oxidant, which were used to reinforce polyvinyl alcohol (PVA).					
31684724	6	57	theme	motions	1349:1355	arg1	monitoring					1312:1321	real-time monitoring	1302:1321	real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse)	1302:1409	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	58	theme	sensing	1278:1284	arg1	performance					1286:1296	superior sensing performance	1269:1296	superior sensing performance	1269:1296	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	3	59	theme	components	608:617	arg1	combination					516:526	The combination	512:526	The combination of weak H-bonds and iron coordination bonds	512:570	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	59	theme	components	608:617	arg1	effect					592:597	the synergistic effect	576:597	the synergistic effect of these components	576:617	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	5	60	theme	flexible	1033:1040	arg1	nanocomposites					1042:1055	the flexible nanocomposites	1029:1055	the flexible nanocomposites	1029:1055	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	3	61	dep	407.1	753:757	arg1	to					750:751	to	750:751	to	750:751	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	1	62	from	interest	249:256	arg1	materials					271:279	composite materials	261:279	composite materials that possess both flexibility and sensing properties	261:332	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	3	63	theme	self-healing	627:638	arg1	films					654:658	self-healing nanocomposite films	627:658	self-healing nanocomposite films	627:658	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	64	theme	nanocomposite	640:652	arg1	films					654:658	self-healing nanocomposite films	627:658	self-healing nanocomposite films	627:658	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	7	65	theme	wearable	1500:1507	arg1	devices					1520:1526	wearable electronic devices	1500:1526	wearable electronic devices	1500:1526	and wearable electronic devices.					
31684724	3	66	theme	coordination	553:564	arg1	bonds					566:570	iron coordination bonds	548:570	iron coordination bonds	548:570	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	67	theme	%	696:696	arg1	increase					698:705	409% increase	693:705	409% increase compared to pure PVA and high toughness up to 407.1%	693:758	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	68	theme	robust	665:670	arg1	strength					683:690	robust mechanical strength	665:690	robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%)	665:759	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	5	69	theme	ionic	1198:1202	arg1	sensors					1224:1230	ionic hydrogel-based skin sensors	1198:1230	ionic hydrogel-based skin sensors	1198:1230	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	1	70	theme	biomedical	160:169	arg1	sensors					171:177	biomedical sensors	160:177	biomedical sensors	160:177	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	5	71	theme	hydrogel-based	1204:1217	arg1	sensors					1224:1230	ionic hydrogel-based skin sensors	1198:1230	ionic hydrogel-based skin sensors	1198:1230	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	3	72	theme	own	813:815	arg1	weight					817:822	its own weight	809:822	its own weight	809:822	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	6	73	theme	subtle	1336:1341	arg1	motions					1349:1355	large and subtle human motions	1326:1355	large and subtle human motions (finger bending motions, swallowing, and wrist pulse)	1326:1409	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	4	74	dep	conductive	946:955	arg1	%					967:967	54.9-91.2%	958:967	54.9-91.2%	958:967	When damaged, the nanocomposite films displayed good mechanical (72.0-76.3%) and conductive (54.9-91.2%) recovery after a healing time of 30 min.					
31684724	1	75	contain	possess	286:292	arg2	flexibility					299:309	flexibility	299:309	flexibility	299:309	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	1	75	contain	possess	286:292	arg1	materials					271:279	composite materials	261:279	composite materials that possess both flexibility and sensing properties	261:332	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	1	75	contain	possess	286:292	arg2	properties					323:332	sensing properties	315:332	sensing properties	315:332	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	6	76	theme	skin	1482:1485	arg1	sensors					1487:1493	flexible skin sensors	1473:1493	flexible skin sensors	1473:1493	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	3	77	theme	H-bonds	536:542	arg1	combination					516:526	The combination	512:526	The combination of weak H-bonds and iron coordination bonds	512:570	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	3	77	theme	H-bonds	536:542	arg1	effect					592:597	the synergistic effect	576:597	the synergistic effect of these components	576:617	The combination of weak H-bonds and iron coordination bonds and the synergistic effect of these components yielded self-healing nanocomposite films with robust mechanical strength (409% increase compared to pure PVA and high toughness up to 407.1%) and excellent adhesion (9670 times greater than its own weight) to various substrates in air and water.					
31684724	5	78	theme	skin	1219:1222	arg1	sensors					1224:1230	ionic hydrogel-based skin sensors	1198:1230	ionic hydrogel-based skin sensors	1198:1230	More importantly, the flexible nanocomposites possessed high strain sensitivity under subtle strains (<48.5%) with a gauge factor (GF) of 2.52, which was relatively larger than the GF of ionic hydrogel-based skin sensors.					
31684724	1	79	theme	composite	261:269	arg1	materials					271:279	composite materials	261:279	composite materials that possess both flexibility and sensing properties	261:332	Electronic skins are developed for applications such as biomedical sensors, robotic prosthetics, and human-machine interactions, which raise the interest in composite materials that possess both flexibility and sensing properties.					
31684724	0	80	theme	Sensor	88:93	arg1	Systems					95:101	Biocompatible Electronic Skin Sensor Systems	58:101	Biocompatible Electronic Skin Sensor Systems	58:101	Self-Healable Conductive Nanocellulose Nanocomposites for Biocompatible Electronic Skin Sensor Systems.					
31684724	6	81	theme	bending	1365:1371	arg1	motions					1373:1379	finger bending motions	1358:1379	finger bending motions	1358:1379	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	82	theme	flexible	1473:1480	arg1	sensors					1487:1493	flexible skin sensors	1473:1493	flexible skin sensors	1473:1493	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
31684724	6	83	theme	real-time	1302:1310	arg1	monitoring					1312:1321	real-time monitoring	1302:1321	real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse)	1302:1409	These nanocomposite films possessed superior sensing performance for real-time monitoring of large and subtle human motions (finger bending motions, swallowing, and wrist pulse); thus, they have great potentials in health monitoring, smart flexible skin sensors.					
30901846	8	0	theme	volunteers	1367:1376	arg1	diets					1340:1344	the diets	1336:1344	the diets (3 g/day) of healthy volunteers (n = 17) for 28 days	1336:1397	Alginate was supplemented into the diets (3 g/day) of healthy volunteers (n = 17) for 28 days.					
30901846	8	0	theme	volunteers	1367:1376	arg1	g/day					1349:1353	3 g/day	1347:1353	3 g/day	1347:1353	Alginate was supplemented into the diets (3 g/day) of healthy volunteers (n = 17) for 28 days.					
30901846	5	1	theme	iron	851:854	arg1	excess					856:861	iron excess	851:861	iron excess	851:861	Considering that iron excess is able to negatively alter the microbiome, the use of alginate as a food supplement could be useful in colonic-iron chelation.					
30901846	11	2	theme	enzymatic	1787:1795	arg1	degradation					1797:1807	bacterial or enzymatic degradation	1774:1807	bacterial or enzymatic degradation within the gastro-intestinal tract	1774:1842	The bacterial communities within the SHIME model were also not influenced by iron and or alginate; it is possible that alginate may be susceptible to bacterial or enzymatic degradation within the gastro-intestinal tract.					
30901846	5	3	from	chelation	980:988	arg1	use					911:913	the use	907:913	the use of alginate as a food supplement	907:946	Considering that iron excess is able to negatively alter the microbiome, the use of alginate as a food supplement could be useful in colonic-iron chelation.					
30901846	5	3	from	chelation	980:988	arg1	useful					957:962	useful	957:962	useful	957:962	Considering that iron excess is able to negatively alter the microbiome, the use of alginate as a food supplement could be useful in colonic-iron chelation.					
30901846	3	4	theme	communities	649:659	arg1	viability					626:634	the viability	622:634	the viability of bacterial communities within the colon	622:676	Dietary fibres are commonly used in pre-biotic supplements to beneficially affect the host by improving the viability of bacterial communities within the colon.					
30901846	8	5	theme	healthy	1359:1365	arg1	n					1379:1379	n = 17	1379:1384	n = 17	1379:1384	Alginate was supplemented into the diets (3 g/day) of healthy volunteers (n = 17) for 28 days.					
30901846	8	5	theme	healthy	1359:1365	arg1	volunteers					1367:1376	healthy volunteers	1359:1376	healthy volunteers (n = 17)	1359:1385	Alginate was supplemented into the diets (3 g/day) of healthy volunteers (n = 17) for 28 days.					
30901846	11	6	theme	or	1710:1711	arg1	alginate					1713:1720	or alginate	1710:1720	or alginate	1710:1720	The bacterial communities within the SHIME model were also not influenced by iron and or alginate; it is possible that alginate may be susceptible to bacterial or enzymatic degradation within the gastro-intestinal tract.					
30901846	11	7	theme	bacterial	1628:1636	arg1	communities					1638:1648	The bacterial communities	1624:1648	The bacterial communities within the SHIME model	1624:1671	The bacterial communities within the SHIME model were also not influenced by iron and or alginate; it is possible that alginate may be susceptible to bacterial or enzymatic degradation within the gastro-intestinal tract.					
30901846	2	8	theme	microbial	482:490	arg1	changes					492:498	these microbial changes	476:498	these microbial changes	476:498	A microbial dysbiosis is observed in IBD patients, and intra-luminal colonic-iron is able to support a more pathogenic community of bacteria; whether this is attributed to the development of IBD and how iron could be mediating these microbial changes is still unknown.					
30901846	11	9	theme	gastro-intestinal	1820:1836	arg1	tract					1838:1842	the gastro-intestinal tract	1816:1842	the gastro-intestinal tract	1816:1842	The bacterial communities within the SHIME model were also not influenced by iron and or alginate; it is possible that alginate may be susceptible to bacterial or enzymatic degradation within the gastro-intestinal tract.					
30901846	6	10	theme	iron	1081:1084	arg1	chelator					1086:1093	a dietary iron chelator	1071:1093	a dietary iron chelator	1071:1093	As such, this first-in-man study aimed to assess whether the use of alginate as a dietary iron chelator was both safe and well tolerated.					
30901846	4	11	theme	biopolymers	704:714	arg1	Alginates					679:687	Alginates	679:687	Alginates	679:687	Alginates are a class of biopolymers considered as prebiotics due to their fibre-like composition and are able to bind metal cations, in particular, iron.					
30901846	4	11	theme	biopolymers	704:714	arg1	class					695:699	a class	693:699	a class of biopolymers considered as prebiotics due to their fibre-like composition	693:775	Alginates are a class of biopolymers considered as prebiotics due to their fibre-like composition and are able to bind metal cations, in particular, iron.					
30901846	9	12	theme	minor	1498:1502	arg1	effects					1509:1515	very minor side effects	1493:1515	very minor side effects	1493:1515	Results from this study suggest that daily ingestion of 3 g alginate was well tolerated with very minor side effects.					
30901846	9	13	theme	g	1458:1458	arg1	alginate					1460:1467	3 g alginate	1456:1467	3 g alginate	1456:1467	Results from this study suggest that daily ingestion of 3 g alginate was well tolerated with very minor side effects.					
30901846	0	14	theme	Participant	94:104	arg1	Study					106:110	A Healthy Participant Study	84:110	A Healthy Participant Study	84:110	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	7	15	theme	microbiome	1172:1181	arg1	levels					1192:1197	the microbiome and iron levels	1168:1197	levels	1192:1197	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	9	16	theme	side	1504:1507	arg1	effects					1509:1515	very minor side effects	1493:1515	very minor side effects	1493:1515	Results from this study suggest that daily ingestion of 3 g alginate was well tolerated with very minor side effects.					
30901846	7	17	dep	SHIME	1241:1245	arg1	Simulation					1248:1257	Simulation	1248:1257	an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem)	1221:1302	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	2	18	theme	microbial	251:259	arg1	dysbiosis					261:269	A microbial dysbiosis	249:269	A microbial dysbiosis	249:269	A microbial dysbiosis is observed in IBD patients, and intra-luminal colonic-iron is able to support a more pathogenic community of bacteria; whether this is attributed to the development of IBD and how iron could be mediating these microbial changes is still unknown.					
30901846	7	19	theme	model	1235:1239	arg1	SHIME					1241:1245	an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem)	1221:1302	an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem)	1221:1302	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	1	20	theme	iron	159:162	arg1	nature					149:154	the ferro-toxic nature	133:154	the ferro-toxic nature of iron	133:162	Evidence supporting the ferro-toxic nature of iron in the progression of inflammatory bowel disease (IBD) is becoming well established.					
30901846	0	21	theme	Healthy	86:92	arg1	Study					106:110	A Healthy Participant Study	84:110	A Healthy Participant Study	84:110	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	4	22	theme	metal	798:802	arg1	cations					804:810	metal cations	798:810	metal cations	798:810	Alginates are a class of biopolymers considered as prebiotics due to their fibre-like composition and are able to bind metal cations, in particular, iron.					
30901846	4	23	theme	due	741:743	arg1	prebiotics					730:739	prebiotics	730:739	prebiotics due to their fibre-like composition	730:775	Alginates are a class of biopolymers considered as prebiotics due to their fibre-like composition and are able to bind metal cations, in particular, iron.					
30901846	2	24	theme	pathogenic	357:366	arg1	community					368:376	a more pathogenic community	350:376	a more pathogenic community of bacteria	350:388	A microbial dysbiosis is observed in IBD patients, and intra-luminal colonic-iron is able to support a more pathogenic community of bacteria; whether this is attributed to the development of IBD and how iron could be mediating these microbial changes is still unknown.					
30901846	7	25	theme	iron	1187:1190	arg1	levels					1192:1197	the microbiome and iron levels	1168:1197	levels	1192:1197	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	6	26	theme	dietary	1073:1079	arg1	chelator					1086:1093	a dietary iron chelator	1071:1093	a dietary iron chelator	1071:1093	As such, this first-in-man study aimed to assess whether the use of alginate as a dietary iron chelator was both safe and well tolerated.					
30901846	8	27	theme	=	1381:1381	arg1	n					1379:1379	n = 17	1379:1384	n = 17	1379:1384	Alginate was supplemented into the diets (3 g/day) of healthy volunteers (n = 17) for 28 days.					
30901846	8	27	theme	=	1381:1381	arg1	volunteers					1367:1376	healthy volunteers	1359:1376	healthy volunteers (n = 17)	1359:1385	Alginate was supplemented into the diets (3 g/day) of healthy volunteers (n = 17) for 28 days.					
30901846	2	28	theme	IBD	286:288	arg1	patients					290:297	IBD patients	286:297	IBD patients	286:297	A microbial dysbiosis is observed in IBD patients, and intra-luminal colonic-iron is able to support a more pathogenic community of bacteria; whether this is attributed to the development of IBD and how iron could be mediating these microbial changes is still unknown.					
30901846	5	29	from	useful	957:962	arg1	chelation					980:988	colonic-iron chelation	967:988	colonic-iron chelation	967:988	Considering that iron excess is able to negatively alter the microbiome, the use of alginate as a food supplement could be useful in colonic-iron chelation.					
30901846	5	30	theme	food	932:935	arg1	supplement					937:946	a food supplement	930:946	a food supplement	930:946	Considering that iron excess is able to negatively alter the microbiome, the use of alginate as a food supplement could be useful in colonic-iron chelation.					
30901846	11	31	theme	bacterial	1774:1782	arg1	degradation					1797:1807	bacterial or enzymatic degradation	1774:1807	bacterial or enzymatic degradation within the gastro-intestinal tract	1774:1842	The bacterial communities within the SHIME model were also not influenced by iron and or alginate; it is possible that alginate may be susceptible to bacterial or enzymatic degradation within the gastro-intestinal tract.					
30901846	7	32	theme	Microbial	1283:1291	arg1	Ecosystem					1293:1301	the Human Intestinal Microbial Ecosystem	1262:1301	the Human Intestinal Microbial Ecosystem	1262:1301	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	9	33	theme	daily	1437:1441	arg1	ingestion					1443:1451	daily ingestion	1437:1451	daily ingestion of 3 g alginate	1437:1467	Results from this study suggest that daily ingestion of 3 g alginate was well tolerated with very minor side effects.					
30901846	2	34	located	observed	274:281	arg2	dysbiosis					261:269	A microbial dysbiosis	249:269	A microbial dysbiosis	249:269	A microbial dysbiosis is observed in IBD patients, and intra-luminal colonic-iron is able to support a more pathogenic community of bacteria; whether this is attributed to the development of IBD and how iron could be mediating these microbial changes is still unknown.					
30901846	2	34	located	observed	274:281	arg1	patients					290:297	IBD patients	286:297	IBD patients	286:297	A microbial dysbiosis is observed in IBD patients, and intra-luminal colonic-iron is able to support a more pathogenic community of bacteria; whether this is attributed to the development of IBD and how iron could be mediating these microbial changes is still unknown.					
30901846	0	35	theme	Study	106:110	arg1	Results					73:79	Results	73:79	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.	0:111	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	6	36	dep	both	1099:1102	arg1	safe					1104:1107	safe	1104:1107	safe	1104:1107	As such, this first-in-man study aimed to assess whether the use of alginate as a dietary iron chelator was both safe and well tolerated.					
30901846	6	36	dep	both	1099:1102	arg1	use					1052:1054	the use	1048:1054	the use of alginate as a dietary iron chelator	1048:1093	As such, this first-in-man study aimed to assess whether the use of alginate as a dietary iron chelator was both safe and well tolerated.					
30901846	6	36	dep	both	1099:1102	arg1	both					1099:1102	both	1099:1102	both	1099:1102	As such, this first-in-man study aimed to assess whether the use of alginate as a dietary iron chelator was both safe and well tolerated.					
30901846	3	37	theme	Dietary	518:524	arg1	fibres					526:531	Dietary fibres	518:531	Dietary fibres	518:531	Dietary fibres are commonly used in pre-biotic supplements to beneficially affect the host by improving the viability of bacterial communities within the colon.					
30901846	7	38	from	impact	1146:1151	arg1	levels					1192:1197	the microbiome and iron levels	1168:1197	levels	1192:1197	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	1	39	theme	inflammatory	186:197	arg1	IBD					214:216	IBD	214:216	IBD	214:216	Evidence supporting the ferro-toxic nature of iron in the progression of inflammatory bowel disease (IBD) is becoming well established.					
30901846	1	39	theme	inflammatory	186:197	arg1	disease					205:211	inflammatory bowel disease	186:211	inflammatory bowel disease (IBD)	186:217	Evidence supporting the ferro-toxic nature of iron in the progression of inflammatory bowel disease (IBD) is becoming well established.					
30901846	2	40	theme	IBD	440:442	arg1	development					425:435	the development	421:435	the development of IBD	421:442	A microbial dysbiosis is observed in IBD patients, and intra-luminal colonic-iron is able to support a more pathogenic community of bacteria; whether this is attributed to the development of IBD and how iron could be mediating these microbial changes is still unknown.					
30901846	10	41	theme	detrimental	1532:1542	arg1	changes					1544:1550	no detrimental changes	1529:1550	no detrimental changes	1529:1550	There were no detrimental changes in a variety of haematological parameters or the intestinal microbiome.					
30901846	1	42	theme	bowel	199:203	arg1	IBD					214:216	IBD	214:216	IBD	214:216	Evidence supporting the ferro-toxic nature of iron in the progression of inflammatory bowel disease (IBD) is becoming well established.					
30901846	1	42	theme	bowel	199:203	arg1	disease					205:211	inflammatory bowel disease	186:211	inflammatory bowel disease (IBD)	186:217	Evidence supporting the ferro-toxic nature of iron in the progression of inflammatory bowel disease (IBD) is becoming well established.					
30901846	7	43	theme	Human	1266:1270	arg1	Ecosystem					1293:1301	the Human Intestinal Microbial Ecosystem	1262:1301	the Human Intestinal Microbial Ecosystem	1262:1301	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	9	44	from	study	1418:1422	arg1	Results					1400:1406	Results	1400:1406	Results from this study	1400:1422	Results from this study suggest that daily ingestion of 3 g alginate was well tolerated with very minor side effects.					
30901846	2	45	theme	bacteria	381:388	arg1	community					368:376	a more pathogenic community	350:376	a more pathogenic community of bacteria	350:388	A microbial dysbiosis is observed in IBD patients, and intra-luminal colonic-iron is able to support a more pathogenic community of bacteria; whether this is attributed to the development of IBD and how iron could be mediating these microbial changes is still unknown.					
30901846	6	46	theme	first-in-man	1005:1016	arg1	study					1018:1022	this first-in-man study	1000:1022	this first-in-man study	1000:1022	As such, this first-in-man study aimed to assess whether the use of alginate as a dietary iron chelator was both safe and well tolerated.					
30901846	3	47	theme	pre-biotic	554:563	arg1	supplements					565:575	pre-biotic supplements	554:575	pre-biotic supplements	554:575	Dietary fibres are commonly used in pre-biotic supplements to beneficially affect the host by improving the viability of bacterial communities within the colon.					
30901846	1	48	theme	disease	205:211	arg1	progression					171:181	the progression	167:181	the progression of inflammatory bowel disease (IBD)	167:217	Evidence supporting the ferro-toxic nature of iron in the progression of inflammatory bowel disease (IBD) is becoming well established.					
30901846	0	49	theme	Alginate-Based	43:56	arg1	Chelator					63:70	a Potential Alginate-Based Iron Chelator	31:70	a Potential Alginate-Based Iron Chelator	31:70	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	3	50	used	used	546:549	arg2	fibres					526:531	Dietary fibres	518:531	Dietary fibres	518:531	Dietary fibres are commonly used in pre-biotic supplements to beneficially affect the host by improving the viability of bacterial communities within the colon.					
30901846	0	51	theme	Potential	33:41	arg1	Chelator					63:70	a Potential Alginate-Based Iron Chelator	31:70	a Potential Alginate-Based Iron Chelator	31:70	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	11	52	theme	SHIME	1661:1665	arg1	model					1667:1671	the SHIME model	1657:1671	the SHIME model	1657:1671	The bacterial communities within the SHIME model were also not influenced by iron and or alginate; it is possible that alginate may be susceptible to bacterial or enzymatic degradation within the gastro-intestinal tract.					
30901846	10	53	theme	haematological	1568:1581	arg1	parameters					1583:1592	haematological parameters	1568:1592	haematological parameters	1568:1592	There were no detrimental changes in a variety of haematological parameters or the intestinal microbiome.					
30901846	5	54	theme	colonic-iron	967:978	arg1	chelation					980:988	colonic-iron chelation	967:988	colonic-iron chelation	967:988	Considering that iron excess is able to negatively alter the microbiome, the use of alginate as a food supplement could be useful in colonic-iron chelation.					
30901846	10	55	theme	parameters	1583:1592	arg1	microbiome					1612:1621	the intestinal microbiome	1597:1621	the intestinal microbiome	1597:1621	There were no detrimental changes in a variety of haematological parameters or the intestinal microbiome.					
30901846	10	55	theme	parameters	1583:1592	arg1	variety					1557:1563	a variety	1555:1563	a variety of haematological parameters or the intestinal microbiome	1555:1621	There were no detrimental changes in a variety of haematological parameters or the intestinal microbiome.					
30901846	10	55	theme	parameters	1583:1592	arg1	parameters					1583:1592	haematological parameters	1568:1592	haematological parameters	1568:1592	There were no detrimental changes in a variety of haematological parameters or the intestinal microbiome.					
30901846	2	56	theme	intra-luminal	304:316	arg1	colonic-iron					318:329	intra-luminal colonic-iron	304:329	intra-luminal colonic-iron	304:329	A microbial dysbiosis is observed in IBD patients, and intra-luminal colonic-iron is able to support a more pathogenic community of bacteria; whether this is attributed to the development of IBD and how iron could be mediating these microbial changes is still unknown.					
30901846	10	57	theme	intestinal	1601:1610	arg1	microbiome					1612:1621	the intestinal microbiome	1597:1621	the intestinal microbiome	1597:1621	There were no detrimental changes in a variety of haematological parameters or the intestinal microbiome.					
30901846	3	58	theme	bacterial	639:647	arg1	communities					649:659	bacterial communities	639:659	bacterial communities within the colon	639:676	Dietary fibres are commonly used in pre-biotic supplements to beneficially affect the host by improving the viability of bacterial communities within the colon.					
30901846	4	59	theme	fibre-like	754:763	arg1	composition					765:775	their fibre-like composition	748:775	their fibre-like composition	748:775	Alginates are a class of biopolymers considered as prebiotics due to their fibre-like composition and are able to bind metal cations, in particular, iron.					
30901846	7	60	theme	Intestinal	1272:1281	arg1	Ecosystem					1293:1301	the Human Intestinal Microbial Ecosystem	1262:1301	the Human Intestinal Microbial Ecosystem	1262:1301	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	10	61	theme	microbiome	1612:1621	arg1	microbiome					1612:1621	the intestinal microbiome	1597:1621	the intestinal microbiome	1597:1621	There were no detrimental changes in a variety of haematological parameters or the intestinal microbiome.					
30901846	10	61	theme	microbiome	1612:1621	arg1	variety					1557:1563	a variety	1555:1563	a variety of haematological parameters or the intestinal microbiome	1555:1621	There were no detrimental changes in a variety of haematological parameters or the intestinal microbiome.					
30901846	10	61	theme	microbiome	1612:1621	arg1	parameters					1583:1592	haematological parameters	1568:1592	haematological parameters	1568:1592	There were no detrimental changes in a variety of haematological parameters or the intestinal microbiome.					
30901846	7	62	theme	Ecosystem	1293:1301	arg1	SHIME					1241:1245	an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem)	1221:1302	an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem)	1221:1302	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	5	63	theme	alginate	918:925	arg1	use					911:913	the use	907:913	the use of alginate as a food supplement	907:946	Considering that iron excess is able to negatively alter the microbiome, the use of alginate as a food supplement could be useful in colonic-iron chelation.					
30901846	5	63	theme	alginate	918:925	arg1	useful					957:962	useful	957:962	useful	957:962	Considering that iron excess is able to negatively alter the microbiome, the use of alginate as a food supplement could be useful in colonic-iron chelation.					
30901846	1	64	dep	becoming	222:229	arg1	established					236:246	established	236:246	is becoming well established	219:246	Evidence supporting the ferro-toxic nature of iron in the progression of inflammatory bowel disease (IBD) is becoming well established.					
30901846	9	65	theme	alginate	1460:1467	arg1	ingestion					1443:1451	daily ingestion	1437:1451	daily ingestion of 3 g alginate	1437:1467	Results from this study suggest that daily ingestion of 3 g alginate was well tolerated with very minor side effects.					
30901846	7	66	theme	alginate	1156:1163	arg1	impact					1146:1151	the impact	1142:1151	the impact of alginate on the microbiome and iron levels	1142:1197	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	0	67	theme	Chelator	63:70	arg1	Tolerability					15:26	Tolerability	15:26	Tolerability	15:26	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	0	67	theme	Chelator	63:70	arg1	Safety					4:9	Safety	4:9	Safety	4:9	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	0	68	dep	Safety	4:9	arg1	The					0:2	The	0:2	The	0:2	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	0	68	dep	Safety	4:9	arg1	Results					73:79	Results	73:79	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.	0:111	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	7	69	theme	intestinal	1224:1233	arg1	SHIME					1241:1245	an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem)	1221:1302	an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem)	1221:1302	In addition, the impact of alginate on the microbiome and iron levels was assessed by using an intestinal model SHIME (Simulation of the Human Intestinal Microbial Ecosystem).					
30901846	0	70	theme	Iron	58:61	arg1	Chelator					63:70	a Potential Alginate-Based Iron Chelator	31:70	a Potential Alginate-Based Iron Chelator	31:70	The Safety and Tolerability of a Potential Alginate-Based Iron Chelator; Results of A Healthy Participant Study.					
30901846	6	71	theme	alginate	1059:1066	arg1	safe					1104:1107	safe	1104:1107	safe	1104:1107	As such, this first-in-man study aimed to assess whether the use of alginate as a dietary iron chelator was both safe and well tolerated.					
30901846	6	71	theme	alginate	1059:1066	arg1	use					1052:1054	the use	1048:1054	the use of alginate as a dietary iron chelator	1048:1093	As such, this first-in-man study aimed to assess whether the use of alginate as a dietary iron chelator was both safe and well tolerated.					
30901846	6	71	theme	alginate	1059:1066	arg1	both					1099:1102	both	1099:1102	both	1099:1102	As such, this first-in-man study aimed to assess whether the use of alginate as a dietary iron chelator was both safe and well tolerated.					
30901846	1	72	theme	ferro-toxic	137:147	arg1	nature					149:154	the ferro-toxic nature	133:154	the ferro-toxic nature of iron	133:162	Evidence supporting the ferro-toxic nature of iron in the progression of inflammatory bowel disease (IBD) is becoming well established.					
30776438	6	0	theme	physicochemical	732:746	arg1	properties					748:757	The physicochemical properties	728:757	The physicochemical properties of PHC film	728:769	The physicochemical properties of PHC film were verified, including structure, morphological analysis, degradation, swelling, electrical properties and so on.					
30776438	2	1	theme	trichloride	370:380	arg1	catalysis					350:358	the catalysis	346:358	the catalysis of ferric trichloride	346:380	Heparin was initially modified with pyrrole, which was further polymerized with pyrrole monomer under the catalysis of ferric trichloride.					
30776438	7	2	theme	promotion	905:913	arg1	results					915:921	the promotion results	901:921	the promotion results of pheochromocytoma cells growing	901:955	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
30776438	2	3	theme	ferric	363:368	arg1	trichloride					370:380	ferric trichloride	363:380	ferric trichloride	363:380	Heparin was initially modified with pyrrole, which was further polymerized with pyrrole monomer under the catalysis of ferric trichloride.					
30776438	2	4	with	polymerized	307:317	arg1	monomer					332:338	pyrrole monomer	324:338	pyrrole monomer under the catalysis of ferric trichloride	324:380	Heparin was initially modified with pyrrole, which was further polymerized with pyrrole monomer under the catalysis of ferric trichloride.					
30776438	7	5	theme	promising	992:1000	arg1	alternative					1002:1012	a promising alternative	990:1012	a promising alternative for nerve regeneration	990:1035	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
30776438	7	5	theme	promising	992:1000	arg1	film					967:970	this PHC film	958:970	this PHC film	958:970	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
30776438	0	6	theme	composite	65:73	arg1	film					86:89	collagen-heparin-polypyrrole composite conductive film	36:89	collagen-heparin-polypyrrole composite conductive film	36:89	Fabrication and characterization of collagen-heparin-polypyrrole composite conductive film for neural scaffold.					
30776438	6	7	theme	morphological	807:819	arg1	analysis					821:828	morphological analysis	807:828	morphological analysis	807:828	The physicochemical properties of PHC film were verified, including structure, morphological analysis, degradation, swelling, electrical properties and so on.					
30776438	7	8	theme	PHC	963:965	arg1	alternative					1002:1012	a promising alternative	990:1012	a promising alternative for nerve regeneration	990:1035	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
30776438	7	8	theme	PHC	963:965	arg1	film					967:970	this PHC film	958:970	this PHC film	958:970	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
30776438	7	9	theme	growing	949:955	arg1	cells					943:947	pheochromocytoma cells growing	926:955	pheochromocytoma cells growing	926:955	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
30776438	2	10	theme	pyrrole	324:330	arg1	monomer					332:338	pyrrole monomer	324:338	pyrrole monomer under the catalysis of ferric trichloride	324:380	Heparin was initially modified with pyrrole, which was further polymerized with pyrrole monomer under the catalysis of ferric trichloride.					
30776438	5	11	theme	cells	714:718	arg1	growth					720:725	nerve cells growth	708:725	nerve cells growth	708:725	Additionally, heparin was verified to promote nerve cells growth.					
30776438	4	12	theme	extracellular	640:652	arg1	matrix					654:659	the extracellular matrix	636:659	the extracellular matrix	636:659	In this system, heparin and collagen contributed to improving the biocompatibility, because they were the major component of the extracellular matrix.					
30776438	3	13	with	interaction	463:473	arg1	pyrrole					480:486	pyrrole	480:486	pyrrole	480:486	Then collagen was added and crosslinked through amide bond, as well as physical interaction with pyrrole through hydrogen bond.					
30776438	7	14	theme	nerve	1018:1022	arg1	regeneration					1024:1035	nerve regeneration	1018:1035	nerve regeneration	1018:1035	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
30776438	1	15	theme	PHC	187:189	arg1	polypyrrole-heparin-collagen					157:184	polypyrrole-heparin-collagen	157:184	polypyrrole-heparin-collagen (PHC film)	157:195	In this work, a conductive film consisted of polypyrrole-heparin-collagen (PHC film) was fabricated as a potential neural scaffold.					
30776438	1	15	theme	PHC	187:189	arg1	film					191:194	PHC film	187:194	PHC film	187:194	In this work, a conductive film consisted of polypyrrole-heparin-collagen (PHC film) was fabricated as a potential neural scaffold.					
30776438	3	16	theme	amide	431:435	arg1	bond					437:440	amide bond	431:440	amide bond	431:440	Then collagen was added and crosslinked through amide bond, as well as physical interaction with pyrrole through hydrogen bond.					
30776438	1	17	theme	potential	217:225	arg1	film					139:142	a conductive film	126:142	a conductive film consisted of polypyrrole-heparin-collagen (PHC film)	126:195	In this work, a conductive film consisted of polypyrrole-heparin-collagen (PHC film) was fabricated as a potential neural scaffold.					
30776438	1	17	theme	potential	217:225	arg1	scaffold					234:241	a potential neural scaffold	215:241	a potential neural scaffold	215:241	In this work, a conductive film consisted of polypyrrole-heparin-collagen (PHC film) was fabricated as a potential neural scaffold.					
30776438	3	18	theme	physical	454:461	arg1	interaction					463:473	physical interaction	454:473	physical interaction with pyrrole	454:486	Then collagen was added and crosslinked through amide bond, as well as physical interaction with pyrrole through hydrogen bond.					
30776438	0	19	theme	collagen-heparin-polypyrrole	36:63	arg1	film					86:89	collagen-heparin-polypyrrole composite conductive film	36:89	collagen-heparin-polypyrrole composite conductive film	36:89	Fabrication and characterization of collagen-heparin-polypyrrole composite conductive film for neural scaffold.					
30776438	4	20	theme	matrix	654:659	arg1	they					603:606	they	603:606	they	603:606	In this system, heparin and collagen contributed to improving the biocompatibility, because they were the major component of the extracellular matrix.					
30776438	4	20	theme	matrix	654:659	arg1	component					623:631	the major component	613:631	the major component of the extracellular matrix	613:659	In this system, heparin and collagen contributed to improving the biocompatibility, because they were the major component of the extracellular matrix.					
30776438	0	21	theme	film	86:89	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of collagen-heparin-polypyrrole composite conductive film for neural scaffold.					
30776438	0	21	theme	film	86:89	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of collagen-heparin-polypyrrole composite conductive film for neural scaffold.					
30776438	0	22	theme	conductive	75:84	arg1	film					86:89	collagen-heparin-polypyrrole composite conductive film	36:89	collagen-heparin-polypyrrole composite conductive film	36:89	Fabrication and characterization of collagen-heparin-polypyrrole composite conductive film for neural scaffold.					
30776438	1	23	theme	conductive	128:137	arg1	film					139:142	a conductive film	126:142	a conductive film consisted of polypyrrole-heparin-collagen (PHC film)	126:195	In this work, a conductive film consisted of polypyrrole-heparin-collagen (PHC film) was fabricated as a potential neural scaffold.					
30776438	1	23	theme	conductive	128:137	arg1	scaffold					234:241	a potential neural scaffold	215:241	a potential neural scaffold	215:241	In this work, a conductive film consisted of polypyrrole-heparin-collagen (PHC film) was fabricated as a potential neural scaffold.					
30776438	7	24	theme	pheochromocytoma	926:941	arg1	cells					943:947	pheochromocytoma cells growing	926:955	pheochromocytoma cells growing	926:955	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
30776438	6	25	theme	electrical	854:863	arg1	properties					865:874	electrical properties	854:874	electrical properties	854:874	The physicochemical properties of PHC film were verified, including structure, morphological analysis, degradation, swelling, electrical properties and so on.					
30776438	6	26	theme	film	766:769	arg1	properties					748:757	The physicochemical properties	728:757	The physicochemical properties of PHC film	728:769	The physicochemical properties of PHC film were verified, including structure, morphological analysis, degradation, swelling, electrical properties and so on.					
30776438	7	27	theme	cells	943:947	arg1	results					915:921	the promotion results	901:921	the promotion results of pheochromocytoma cells growing	901:955	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
30776438	4	28	theme	major	617:621	arg1	they					603:606	they	603:606	they	603:606	In this system, heparin and collagen contributed to improving the biocompatibility, because they were the major component of the extracellular matrix.					
30776438	4	28	theme	major	617:621	arg1	component					623:631	the major component	613:631	the major component of the extracellular matrix	613:659	In this system, heparin and collagen contributed to improving the biocompatibility, because they were the major component of the extracellular matrix.					
30776438	5	29	theme	nerve	708:712	arg1	cells					714:718	nerve cells	708:718	nerve cells growth	708:725	Additionally, heparin was verified to promote nerve cells growth.					
30776438	6	30	theme	PHC	762:764	arg1	film					766:769	PHC film	762:769	PHC film	762:769	The physicochemical properties of PHC film were verified, including structure, morphological analysis, degradation, swelling, electrical properties and so on.					
30776438	3	31	theme	hydrogen	496:503	arg1	bond					505:508	hydrogen bond	496:508	hydrogen bond	496:508	Then collagen was added and crosslinked through amide bond, as well as physical interaction with pyrrole through hydrogen bond.					
30776438	0	32	theme	neural	95:100	arg1	scaffold					102:109	neural scaffold	95:109	neural scaffold	95:109	Fabrication and characterization of collagen-heparin-polypyrrole composite conductive film for neural scaffold.					
30776438	1	33	theme	neural	227:232	arg1	film					139:142	a conductive film	126:142	a conductive film consisted of polypyrrole-heparin-collagen (PHC film)	126:195	In this work, a conductive film consisted of polypyrrole-heparin-collagen (PHC film) was fabricated as a potential neural scaffold.					
30776438	1	33	theme	neural	227:232	arg1	scaffold					234:241	a potential neural scaffold	215:241	a potential neural scaffold	215:241	In this work, a conductive film consisted of polypyrrole-heparin-collagen (PHC film) was fabricated as a potential neural scaffold.					
30776438	2	34	mod	modified	266:273	arg1	Heparin					244:250	Heparin	244:250	Heparin	244:250	Heparin was initially modified with pyrrole, which was further polymerized with pyrrole monomer under the catalysis of ferric trichloride.					
30776438	2	34	mod	modified	266:273	arg3	pyrrole					280:286	pyrrole	280:286	pyrrole	280:286	Heparin was initially modified with pyrrole, which was further polymerized with pyrrole monomer under the catalysis of ferric trichloride.					
30776438	7	35	with	Combined	887:894	arg1	results					915:921	the promotion results	901:921	the promotion results of pheochromocytoma cells growing	901:955	Combined with the promotion results of pheochromocytoma cells growing, this PHC film is expected to be a promising alternative for nerve regeneration.					
31576900	2	0	theme	interface	530:538	arg1	layer					540:544	cartilage-bone interface layer	515:544	cartilage-bone interface layer	515:544	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	3	1	theme	scaffold	745:752	arg1	bonding					652:658	bonding	652:658	bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold	652:752	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	3	2	theme	extracellular	794:806	arg1	matrix					808:813	an endogenous fibrotic cartilaginous extracellular matrix	757:813	an endogenous fibrotic cartilaginous extracellular matrix	757:813	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	2	3	from	bone	506:509	arg1	terms					453:457	terms	453:457	terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects	453:576	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	3	from	bone	506:509	arg1	composition					476:486	composition	476:486	composition	476:486	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	3	from	bone	506:509	arg1	structure					462:470	structure	462:470	structure	462:470	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	1	4	theme	integrated	264:273	arg1	graft					275:279	an integrated graft	261:279	an integrated graft	261:279	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	2	5	theme	cartilage-bone	515:528	arg1	layer					540:544	cartilage-bone interface layer	515:544	cartilage-bone interface layer	515:544	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	6	dep	cartilage	495:503	arg1	the					491:493	the	491:493	the	491:493	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	3	7	theme	hydrogel	679:686	arg1	bonding					652:658	bonding	652:658	bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold	652:752	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	4	8	from	layer	1016:1020	arg1	integration					985:995	integration	985:995	integration	985:995	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	4	8	from	layer	1016:1020	arg1	transition					970:979	(i) gradient transition	957:979	(i) gradient transition	957:979	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	0	9	theme	osteochondral	103:115	arg1	repair					124:129	osteochondral defect repair	103:129	osteochondral defect repair	103:129	Engineering a multiphasic, integrated graft with a biologically developed cartilage-bone interface for osteochondral defect repair.					
31576900	5	10	theme	graft	1319:1323	arg1	efficacy					1303:1310	the tissue repair efficacy	1285:1310	the tissue repair efficacy of the graft	1285:1323	Industrialization of the graft remains an unsolved challenge as after decellularization the tissue repair efficacy of the graft decreased.					
31576900	4	11	theme	bone	1041:1044	arg1	layer					1046:1050	the subchondral bone layer	1025:1050	the subchondral bone layer	1025:1050	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	3	12	theme	endogenous	760:769	arg1	matrix					808:813	an endogenous fibrotic cartilaginous extracellular matrix	757:813	an endogenous fibrotic cartilaginous extracellular matrix	757:813	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	4	13	theme	rabbit	1171:1176	arg1	model					1190:1194	a rabbit knee defect model	1169:1194	a rabbit knee defect model	1169:1194	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	4	14	theme	knee	1178:1181	arg1	model					1190:1194	a rabbit knee defect model	1169:1194	a rabbit knee defect model	1169:1194	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	2	15	theme	osteochondral	556:568	arg1	defects					570:576	repair osteochondral defects	549:576	repair osteochondral defects	549:576	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	16	from	cartilage	495:503	arg1	terms					453:457	terms	453:457	terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects	453:576	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	16	from	cartilage	495:503	arg1	composition					476:486	composition	476:486	composition	476:486	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	16	from	cartilage	495:503	arg1	structure					462:470	structure	462:470	structure	462:470	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	1	17	theme	repair	178:183	arg1	defects					199:205	repair osteochondral defects	178:205	repair osteochondral defects	178:205	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	0	18	theme	defect	117:122	arg1	repair					124:129	osteochondral defect repair	103:129	osteochondral defect repair	103:129	Engineering a multiphasic, integrated graft with a biologically developed cartilage-bone interface for osteochondral defect repair.					
31576900	4	19	theme	poly	907:910	arg1	scaffold					937:944	the poly(lactic-co-glycolic acid) scaffold	903:944	the poly(lactic-co-glycolic acid) scaffold	903:944	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	6	20	theme	orthopaedic	1500:1510	arg1	surgery					1512:1518	orthopaedic surgery	1500:1518	orthopaedic surgery	1500:1518	Taken together, the multiphasic osteochondral graft repaired the osteochondral defects successfully and has the potential to be applied clinically as an implant in orthopaedic surgery.					
31576900	4	21	theme	defect	1183:1188	arg1	model					1190:1194	a rabbit knee defect model	1169:1194	a rabbit knee defect model	1169:1194	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	1	22	theme	osteochondral	185:197	arg1	defects					199:205	repair osteochondral defects	178:205	repair osteochondral defects	178:205	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	0	23	theme	cartilage-bone	74:87	arg1	interface					89:97	a biologically developed cartilage-bone interface	49:97	a biologically developed cartilage-bone interface for osteochondral defect repair	49:129	Engineering a multiphasic, integrated graft with a biologically developed cartilage-bone interface for osteochondral defect repair.					
31576900	4	24	theme	subchondral	1029:1039	arg1	layer					1046:1050	the subchondral bone layer	1025:1050	the subchondral bone layer	1025:1050	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	3	25	theme	fibrotic	771:778	arg1	matrix					808:813	an endogenous fibrotic cartilaginous extracellular matrix	757:813	an endogenous fibrotic cartilaginous extracellular matrix	757:813	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	2	26	theme	entire	422:427	arg1	tissue					443:448	the entire osteochondral tissue	418:448	the entire osteochondral tissue	418:448	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	4	27	theme	electron	1076:1083	arg1	microscopy					1085:1094	scanning electron microscopy	1067:1094	scanning electron microscopy	1067:1094	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	5	28	theme	unsolved	1239:1246	arg1	challenge					1248:1256	an unsolved challenge	1236:1256	an unsolved challenge	1236:1256	Industrialization of the graft remains an unsolved challenge as after decellularization the tissue repair efficacy of the graft decreased.					
31576900	4	29	theme	lactic-co-glycolic	912:929	arg1	poly					907:910	the poly	903:910	the poly(lactic-co-glycolic acid) scaffold	903:944	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	4	29	theme	lactic-co-glycolic	912:929	arg1	acid					931:934	lactic-co-glycolic acid	912:934	lactic-co-glycolic acid	912:934	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	2	30	theme	multiphasic	332:342	arg1	graft					388:392	The multiphasic, functionally integrated tissue engineering graft	328:392	The multiphasic, functionally integrated tissue engineering graft described herein	328:409	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	4	31	theme	repair	1150:1155	arg1	efficacy					1157:1164	(ii) superior tissue repair efficacy	1129:1164	(ii) superior tissue repair efficacy	1129:1164	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	2	32	theme	composition	476:486	arg1	terms					453:457	terms	453:457	terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects	453:576	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	5	33	theme	graft	1222:1226	arg1	Industrialization					1197:1213	Industrialization	1197:1213	Industrialization of the graft	1197:1226	Industrialization of the graft remains an unsolved challenge as after decellularization the tissue repair efficacy of the graft decreased.					
31576900	6	34	theme	osteochondral	1401:1413	arg1	defects					1415:1421	the osteochondral defects	1397:1421	the osteochondral defects	1397:1421	Taken together, the multiphasic osteochondral graft repaired the osteochondral defects successfully and has the potential to be applied clinically as an implant in orthopaedic surgery.					
31576900	6	35	contain	has	1440:1442	arg2	potential					1448:1456	the potential to be applied clinically as an implant in orthopaedic surgery	1444:1518	the potential to be applied clinically as an implant in orthopaedic surgery	1444:1518	Taken together, the multiphasic osteochondral graft repaired the osteochondral defects successfully and has the potential to be applied clinically as an implant in orthopaedic surgery.					
31576900	6	35	contain	has	1440:1442	arg1	graft					1382:1386	the multiphasic osteochondral graft	1352:1386	the multiphasic osteochondral graft	1352:1386	Taken together, the multiphasic osteochondral graft repaired the osteochondral defects successfully and has the potential to be applied clinically as an implant in orthopaedic surgery.					
31576900	4	36	theme	tissue	1143:1148	arg1	efficacy					1157:1164	(ii) superior tissue repair efficacy	1129:1164	(ii) superior tissue repair efficacy	1129:1164	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	2	37	theme	structure	462:470	arg1	terms					453:457	terms	453:457	terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects	453:576	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	0	38	theme	multiphasic	14:24	arg1	graft					38:42	a multiphasic, integrated graft	12:42	a multiphasic, integrated graft with a biologically developed cartilage-bone interface for osteochondral defect repair	12:129	Engineering a multiphasic, integrated graft with a biologically developed cartilage-bone interface for osteochondral defect repair.					
31576900	3	39	theme	cartilaginous	780:792	arg1	matrix					808:813	an endogenous fibrotic cartilaginous extracellular matrix	757:813	an endogenous fibrotic cartilaginous extracellular matrix	757:813	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	1	40	theme	successful	212:221	arg1	reconstruction					223:236	successful reconstruction	212:236	successful reconstruction of different layers in an integrated graft, especially the interface	212:305	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	2	41	theme	tissue	369:374	arg1	graft					388:392	The multiphasic, functionally integrated tissue engineering graft	328:392	The multiphasic, functionally integrated tissue engineering graft described herein	328:409	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	6	42	from	implant	1489:1495	arg1	surgery					1512:1518	orthopaedic surgery	1500:1518	orthopaedic surgery	1500:1518	Taken together, the multiphasic osteochondral graft repaired the osteochondral defects successfully and has the potential to be applied clinically as an implant in orthopaedic surgery.					
31576900	3	43	theme	multiphasic	630:640	arg1	graft					642:646	a multiphasic graft	628:646	a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold	628:752	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	1	44	from	graft	275:279	arg1	reconstruction					223:236	successful reconstruction	212:236	successful reconstruction of different layers in an integrated graft, especially the interface	212:305	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	3	45	theme	cartilaginous	665:677	arg1	hydrogel					679:686	a cartilaginous hydrogel	663:686	a cartilaginous hydrogel	663:686	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	3	46	theme	graft	642:646	arg1	fabrication					613:623	the fabrication	609:623	the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix	609:813	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	1	47	from	reconstruction	223:236	arg1	graft					275:279	an integrated graft	261:279	an integrated graft	261:279	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	2	48	theme	repair	549:554	arg1	defects					570:576	repair osteochondral defects	549:576	repair osteochondral defects	549:576	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	4	49	theme	scanning	1067:1074	arg1	microscopy					1085:1094	scanning electron microscopy	1067:1094	scanning electron microscopy	1067:1094	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	4	50	theme	cartilage	1006:1014	arg1	layer					1016:1020	the cartilage layer	1002:1020	the cartilage layer	1002:1020	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	6	51	theme	osteochondral	1368:1380	arg1	graft					1382:1386	the multiphasic osteochondral graft	1352:1386	the multiphasic osteochondral graft	1352:1386	Taken together, the multiphasic osteochondral graft repaired the osteochondral defects successfully and has the potential to be applied clinically as an implant in orthopaedic surgery.					
31576900	2	52	from	layer	540:544	arg1	terms					453:457	terms	453:457	terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects	453:576	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	52	from	layer	540:544	arg1	composition					476:486	composition	476:486	composition	476:486	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	52	from	layer	540:544	arg1	structure					462:470	structure	462:470	structure	462:470	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	4	53	dep	efficacy	1157:1164	arg1	ii					1130:1131	ii	1130:1131	ii	1130:1131	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	2	54	theme	engineering	376:386	arg1	graft					388:392	The multiphasic, functionally integrated tissue engineering graft	328:392	The multiphasic, functionally integrated tissue engineering graft described herein	328:409	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	6	55	theme	multiphasic	1356:1366	arg1	graft					1382:1386	the multiphasic osteochondral graft	1352:1386	the multiphasic osteochondral graft	1352:1386	Taken together, the multiphasic osteochondral graft repaired the osteochondral defects successfully and has the potential to be applied clinically as an implant in orthopaedic surgery.					
31576900	1	56	theme	Tissue	132:137	arg1	engineering					139:149	Tissue engineering	132:149	Tissue engineering	132:149	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	1	56	theme	Tissue	132:137	arg1	approach					166:173	a promising approach	154:173	a promising approach to repair osteochondral defects	154:205	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	4	57	theme	gradient	961:968	arg1	transition					970:979	(i) gradient transition	957:979	(i) gradient transition	957:979	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	3	58	theme	lactic-co-glycolic	708:725	arg1	poly					703:706	sintered poly	694:706	a sintered poly(lactic-co-glycolic acid) microsphere scaffold	692:752	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	3	58	theme	lactic-co-glycolic	708:725	arg1	acid					727:730	lactic-co-glycolic acid	708:730	lactic-co-glycolic acid	708:730	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	4	59	dep	transition	970:979	arg1	i					958:958	i	958:958	i	958:958	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	1	60	theme	different	241:249	arg1	layers					251:256	different layers	241:256	different layers in an integrated graft	241:279	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	1	60	theme	different	241:249	arg1	interface					297:305	the interface	293:305	especially the interface	282:305	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	1	61	from	layers	251:256	arg1	graft					275:279	an integrated graft	261:279	an integrated graft	261:279	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	3	62	theme	sintered	694:701	arg1	poly					703:706	sintered poly	694:706	a sintered poly(lactic-co-glycolic acid) microsphere scaffold	692:752	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	3	62	theme	sintered	694:701	arg1	acid					727:730	lactic-co-glycolic acid	708:730	lactic-co-glycolic acid	708:730	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	0	63	dep	multiphasic	14:24	arg1	integrated					27:36	integrated	27:36	integrated	27:36	Engineering a multiphasic, integrated graft with a biologically developed cartilage-bone interface for osteochondral defect repair.					
31576900	2	64	from	terms	453:457	arg1	layer					540:544	cartilage-bone interface layer	515:544	cartilage-bone interface layer	515:544	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	64	from	terms	453:457	arg1	bone					506:509	bone	506:509	bone	506:509	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	64	from	terms	453:457	arg1	cartilage					495:503	cartilage	495:503	cartilage	495:503	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	2	65	dep	multiphasic	332:342	arg1	integrated					358:367	integrated	358:367	integrated	358:367	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	4	66	theme	superior	1134:1141	arg1	efficacy					1157:1164	(ii) superior tissue repair efficacy	1129:1164	(ii) superior tissue repair efficacy	1129:1164	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	1	67	theme	layers	251:256	arg1	reconstruction					223:236	successful reconstruction	212:236	successful reconstruction of different layers in an integrated graft, especially the interface	212:305	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	0	68	theme	developed	64:72	arg1	interface					89:97	a biologically developed cartilage-bone interface	49:97	a biologically developed cartilage-bone interface for osteochondral defect repair	49:129	Engineering a multiphasic, integrated graft with a biologically developed cartilage-bone interface for osteochondral defect repair.					
31576900	3	69	theme	poly	703:706	arg1	scaffold					745:752	a sintered poly(lactic-co-glycolic acid) microsphere scaffold	692:752	a sintered poly(lactic-co-glycolic acid) microsphere scaffold	692:752	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	4	70	theme	alginate	871:878	arg1	hydrogel					880:887	the alginate hydrogel	867:887	the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold	867:944	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	4	71	dep	culturing	837:845	arg1	allows					946:951	allows	946:951	culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model	837:1194	We demonstrated that culturing chondrocytes within the alginate hydrogel conjugated to the poly(lactic-co-glycolic acid) scaffold allows for (i) gradient transition and integration from the cartilage layer to the subchondral bone layer as assessed by scanning electron microscopy, histology and biochemistry, and (ii) superior tissue repair efficacy in a rabbit knee defect model.					
31576900	2	72	theme	osteochondral	429:441	arg1	tissue					443:448	the entire osteochondral tissue	418:448	the entire osteochondral tissue	418:448	The multiphasic, functionally integrated tissue engineering graft described herein mimics the entire osteochondral tissue in terms of structure and composition at the cartilage, bone and cartilage-bone interface layer to repair osteochondral defects.					
31576900	5	73	theme	tissue	1289:1294	arg1	efficacy					1303:1310	the tissue repair efficacy	1285:1310	the tissue repair efficacy of the graft	1285:1323	Industrialization of the graft remains an unsolved challenge as after decellularization the tissue repair efficacy of the graft decreased.					
31576900	3	74	theme	microsphere	733:743	arg1	scaffold					745:752	a sintered poly(lactic-co-glycolic acid) microsphere scaffold	692:752	a sintered poly(lactic-co-glycolic acid) microsphere scaffold	692:752	In this manuscript, we report the fabrication of a multiphasic graft via bonding of a cartilaginous hydrogel and a sintered poly(lactic-co-glycolic acid) microsphere scaffold by an endogenous fibrotic cartilaginous extracellular matrix.					
31576900	0	75	with	graft	38:42	arg1	interface					89:97	a biologically developed cartilage-bone interface	49:97	a biologically developed cartilage-bone interface for osteochondral defect repair	49:129	Engineering a multiphasic, integrated graft with a biologically developed cartilage-bone interface for osteochondral defect repair.					
31576900	1	76	theme	promising	156:164	arg1	engineering					139:149	Tissue engineering	132:149	Tissue engineering	132:149	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	1	76	theme	promising	156:164	arg1	approach					166:173	a promising approach	154:173	a promising approach to repair osteochondral defects	154:205	Tissue engineering is a promising approach to repair osteochondral defects, yet successful reconstruction of different layers in an integrated graft, especially the interface remains challenging.					
31576900	5	77	theme	repair	1296:1301	arg1	efficacy					1303:1310	the tissue repair efficacy	1285:1310	the tissue repair efficacy of the graft	1285:1323	Industrialization of the graft remains an unsolved challenge as after decellularization the tissue repair efficacy of the graft decreased.					
29785910	9	0	from	records	1370:1376	arg1	total					1381:1385	total	1381:1385	total	1381:1385	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29785910	13	1	theme	SCC	1802:1804	arg1	absence					1791:1797	the absence	1787:1797	the absence of SCC	1787:1804	In the absence of SCC, lactose concentration featured as the most important variable, followed by EC.					
29785910	13	2	theme	lactose	1807:1813	arg1	concentration					1815:1827	lactose concentration	1807:1827	lactose concentration	1807:1827	In the absence of SCC, lactose concentration featured as the most important variable, followed by EC.					
29785910	3	3	theme	longitudinal	530:541	arg1	study					543:547	a large-scale and longitudinal study	512:547	a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM	512:630	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	6	4	theme	Somatic	862:868	arg1	Cell					870:873	Test-day Somatic Cell	853:873	Test-day Somatic Cell Count (SCC)	853:885	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	0	5	theme	conductivity	197:208	arg1	power					165:169	the predictive power	150:169	the predictive power of lactose and electrical conductivity	150:208	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	3	6	theme	large-scale	514:524	arg1	study					543:547	a large-scale and longitudinal study	512:547	a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM	512:630	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	8	7	theme	efficient	1148:1156	arg1	indicators					1158:1167	additional efficient indicators	1137:1167	additional efficient indicators	1137:1167	Consequently, there would be great benefit to identifying additional efficient indicators from large-scale and longitudinal studies.					
29785910	4	8	theme	prevalent	649:657	arg1	disease					659:665	the most prevalent disease	640:665	the most prevalent disease of dairy cattle	640:681	SCM is the most prevalent disease of dairy cattle, causing substantial economic loss for the dairy industry.					
29785910	4	8	theme	prevalent	649:657	arg1	SCM					633:635	SCM	633:635	SCM	633:635	SCM is the most prevalent disease of dairy cattle, causing substantial economic loss for the dairy industry.					
29785910	2	9	theme	large-scale	300:310	arg1	mining					312:317	large-scale mining	300:317	large-scale mining of milk composition features by pattern recognition models	300:376	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	6	10	theme	SCM	919:921	arg1	Count					875:879	Test-day Somatic Cell Count	853:879	Test-day Somatic Cell Count (SCC)	853:885	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	6	10	theme	SCM	919:921	arg1	indicator					906:914	the most common indicator	890:914	the most common indicator of SCM and the primary mastitis surveillance approach worldwide	890:978	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	11	11	theme	New	1666:1668	arg1	Zealand					1670:1676	New Zealand	1666:1676	New Zealand	1666:1676	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	0	12	theme	milk	110:113	arg1	features					127:134	milk composition features	110:134	milk composition features	110:134	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	5	13	theme	herd	813:816	arg1	health					818:823	herd health	813:823	herd health	813:823	Developing new techniques to diagnose SCM in its early stages improves herd health and is of great importance.					
29785910	11	14	used	used	1564:1567	arg2	cut-offs					1550:1557	Three SCC cut-offs	1540:1557	Three SCC cut-offs	1540:1557	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	0	15	from	cattle	68:73	arg1	indicators					23:32	indicators	23:32	indicators of sub-clinical mastitis in dairy cattle	23:73	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	7	16	dep	major	1043:1047	arg1	concerns					1049:1056	concerns	1049:1056	concerns	1049:1056	However, test-day SCC fluctuates widely between days, causing major concerns for its reliability.					
29785910	0	17	theme	features	127:134	arg1	analysis					98:105	attribute weighting analysis	78:105	attribute weighting analysis of milk composition features	78:134	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	3	18	theme	milk	584:587	arg1	parameters					600:609	various milk production parameters	576:609	various milk production parameters	576:609	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	13	19	theme	important	1850:1858	arg1	variable					1860:1867	the most important variable	1841:1867	the most important variable	1841:1867	In the absence of SCC, lactose concentration featured as the most important variable, followed by EC.					
29785910	4	20	theme	economic	704:711	arg1	loss					713:716	substantial economic loss	692:716	substantial economic loss for the dairy industry	692:739	SCM is the most prevalent disease of dairy cattle, causing substantial economic loss for the dairy industry.					
29785910	7	21	theme	test-day	990:997	arg1	SCC					999:1001	test-day SCC	990:1001	test-day SCC	990:1001	However, test-day SCC fluctuates widely between days, causing major concerns for its reliability.					
29785910	9	22	dep	equipment	1351:1359	arg1	records					1370:1376	346 248 records	1362:1376	346 248 records in total	1362:1385	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29785910	11	23	theme	000	1621:1623	arg1	cells/ml					1625:1632	Australian ≥ 250 000 cells/ml	1604:1632	Australian ≥ 250 000 cells/ml	1604:1632	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	15	24	theme	machine-learning	2055:2070	arg1	systems					2079:2085	machine-learning expert systems	2055:2085	machine-learning expert systems using two or more milk variables (such as lactose concentration and EC)	2055:2157	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC) may produce a predictive pattern for early SCM detection.					
29785910	3	25	theme	parameters	600:609	arg1	ability					565:571	the ability	561:571	the ability of various milk production parameters	561:609	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	3	25	theme	parameters	600:609	arg1	indicators					614:623	indicators	614:623	indicators of SCM	614:630	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	6	26	theme	approach	961:968	arg1	Count					875:879	Test-day Somatic Cell Count	853:879	Test-day Somatic Cell Count (SCC)	853:885	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	6	26	theme	approach	961:968	arg1	indicator					906:914	the most common indicator	890:914	the most common indicator of SCM and the primary mastitis surveillance approach worldwide	890:978	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	0	27	theme	predictive	154:163	arg1	power					165:169	the predictive power	150:169	the predictive power of lactose and electrical conductivity	150:208	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	4	28	theme	cattle	676:681	arg1	disease					659:665	the most prevalent disease	640:665	the most prevalent disease of dairy cattle	640:681	SCM is the most prevalent disease of dairy cattle, causing substantial economic loss for the dairy industry.					
29785910	4	28	theme	cattle	676:681	arg1	SCM					633:635	SCM	633:635	SCM	633:635	SCM is the most prevalent disease of dairy cattle, causing substantial economic loss for the dairy industry.					
29785910	5	29	theme	new	753:755	arg1	techniques					757:766	new techniques	753:766	new techniques	753:766	Developing new techniques to diagnose SCM in its early stages improves herd health and is of great importance.					
29785910	6	30	theme	mastitis	939:946	arg1	approach					961:968	the primary mastitis surveillance approach	927:968	the primary mastitis surveillance approach	927:968	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	11	31	theme	≥200	1644:1647	arg1	cells/ml					1653:1660	European ≥200 000 cells/ml	1635:1660	European ≥200 000 cells/ml	1635:1660	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	0	32	theme	attribute	78:86	arg1	analysis					98:105	attribute weighting analysis	78:105	attribute weighting analysis of milk composition features	78:134	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	15	33	theme	early	2196:2200	arg1	detection					2206:2214	early SCM detection	2196:2214	early SCM detection	2196:2214	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC) may produce a predictive pattern for early SCM detection.					
29785910	10	34	theme	following	1393:1401	arg1	data					1403:1406	The following data	1389:1406	The following data	1389:1406	The following data were analysed: milk volume, protein concentration, lactose concentration, electrical conductivity (EC), milking time and peak flow.					
29785910	2	35	theme	best	395:398	arg1	predictors					400:409	the best predictors	391:409	the best predictors of SCM within the milk composition features	391:453	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	2	36	theme	features	446:453	arg1	predictors					400:409	the best predictors	391:409	the best predictors of SCM within the milk composition features	391:453	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	0	37	from	indicators	23:32	arg1	cattle					68:73	dairy cattle	62:73	dairy cattle	62:73	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	14	38	theme	attribute	1912:1920	arg1	modelling					1931:1939	attribute weighted modelling	1912:1939	attribute weighted modelling	1912:1939	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	15	39	theme	milk	2105:2108	arg1	variables					2110:2118	two or more milk variables	2093:2118	two or more milk variables (such as lactose concentration and EC)	2093:2157	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC) may produce a predictive pattern for early SCM detection.					
29785910	6	40	dep	SCM	919:921	arg1	worldwide					970:978	worldwide	970:978	worldwide	970:978	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	2	41	theme	milk	429:432	arg1	composition					434:444	the milk composition	425:444	the milk composition	425:444	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	4	42	theme	dairy	726:730	arg1	industry					732:739	the dairy industry	722:739	the dairy industry	722:739	SCM is the most prevalent disease of dairy cattle, causing substantial economic loss for the dairy industry.					
29785910	15	43	theme	expert	2072:2077	arg1	systems					2079:2085	machine-learning expert systems	2055:2085	machine-learning expert systems using two or more milk variables (such as lactose concentration and EC)	2055:2157	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC) may produce a predictive pattern for early SCM detection.					
29785910	12	44	theme	Attribute	1721:1729	arg1	AWM					1753:1755	AWM	1753:1755	AWM	1753:1755	At first, 10 different Attribute Weighting Algorithms (AWM) were applied to the data.					
29785910	12	44	theme	Attribute	1721:1729	arg1	Algorithms					1741:1750	10 different Attribute Weighting Algorithms	1708:1750	10 different Attribute Weighting Algorithms (AWM)	1708:1756	At first, 10 different Attribute Weighting Algorithms (AWM) were applied to the data.					
29785910	14	45	theme	first	1894:1898	arg1	time					1900:1903	the first time	1890:1903	the first time	1890:1903	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	11	46	theme	SCM	1599:1601	arg1	prevalence					1585:1594	the prevalence	1581:1594	the prevalence of SCM	1581:1601	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	0	47	theme	indicators	23:32	arg1	study					14:18	A large-scale study	0:18	A large-scale study of indicators of sub-clinical mastitis in dairy cattle	0:73	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	10	48	theme	protein	1436:1442	arg1	concentration					1444:1456	protein concentration	1436:1456	protein concentration	1436:1456	The following data were analysed: milk volume, protein concentration, lactose concentration, electrical conductivity (EC), milking time and peak flow.					
29785910	3	49	theme	SCM	628:630	arg1	ability					565:571	the ability	561:571	the ability of various milk production parameters	561:609	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	3	49	theme	SCM	628:630	arg1	indicators					614:623	indicators	614:623	indicators of SCM	614:630	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	3	50	theme	data	475:478	arg1	algorithms					487:496	data mining algorithms	475:496	data mining algorithms	475:496	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	0	51	theme	sub-clinical	37:48	arg1	mastitis					50:57	sub-clinical mastitis	37:57	sub-clinical mastitis in dairy cattle	37:73	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	2	52	theme	milk	322:325	arg1	composition					327:337	milk composition	322:337	milk composition features	322:346	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	9	53	theme	single	1314:1319	arg1	farm					1321:1324	a single farm	1312:1324	a single farm using in-line electronic equipment (346 248 records in total)	1312:1386	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29785910	9	54	from	farm	1321:1324	arg1	period					1288:1293	a period	1286:1293	a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total)	1286:1386	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29785910	0	55	dep	study	14:18	arg1	analysis					98:105	attribute weighting analysis	78:105	attribute weighting analysis of milk composition features	78:134	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	11	56	theme	Australian	1604:1613	arg1	cells/ml					1625:1632	Australian ≥ 250 000 cells/ml	1604:1632	Australian ≥ 250 000 cells/ml	1604:1632	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	11	57	dep	used	1564:1567	arg1	cells/ml					1653:1660	European ≥200 000 cells/ml	1635:1660	European ≥200 000 cells/ml	1635:1660	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	11	57	dep	used	1564:1567	arg1	cells/ml					1625:1632	Australian ≥ 250 000 cells/ml	1604:1632	Australian ≥ 250 000 cells/ml	1604:1632	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	11	57	dep	used	1564:1567	arg1	Zealand					1670:1676	New Zealand	1666:1676	New Zealand	1666:1676	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	0	58	theme	lactose	174:180	arg1	power					165:169	the predictive power	150:169	the predictive power of lactose and electrical conductivity	150:208	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	2	59	theme	pattern	351:357	arg1	models					371:376	pattern recognition models	351:376	pattern recognition models	351:376	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	1	60	theme	milk	247:250	arg1	composition					252:262	milk composition	247:262	milk composition	247:262	Sub-clinical mastitis (SCM) affects milk composition.					
29785910	9	61	dep	collected	1239:1247	arg1	day					1277:1279	day	1277:1279	day	1277:1279	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29785910	14	62	theme	strong	2011:2016	arg1	concentration					1961:1973	the concentration	1957:1973	the concentration of lactose in milk	1957:1992	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	14	62	theme	strong	2011:2016	arg1	indicator					2018:2026	a strong indicator	2009:2026	a strong indicator of SCM	2009:2033	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	0	63	theme	electrical	186:195	arg1	conductivity					197:208	electrical conductivity	186:208	electrical conductivity	186:208	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	8	64	theme	great	1108:1112	arg1	benefit					1114:1120	great benefit	1108:1120	great benefit to identifying additional efficient indicators from large-scale and longitudinal studies	1108:1209	Consequently, there would be great benefit to identifying additional efficient indicators from large-scale and longitudinal studies.					
29785910	6	65	theme	Cell	870:873	arg1	SCC					882:884	SCC	882:884	SCC	882:884	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	6	65	theme	Cell	870:873	arg1	indicator					906:914	the most common indicator	890:914	the most common indicator of SCM and the primary mastitis surveillance approach worldwide	890:978	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	6	65	theme	Cell	870:873	arg1	Count					875:879	Test-day Somatic Cell Count	853:879	Test-day Somatic Cell Count (SCC)	853:885	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	10	66	theme	electrical	1482:1491	arg1	EC					1507:1508	EC	1507:1508	EC	1507:1508	The following data were analysed: milk volume, protein concentration, lactose concentration, electrical conductivity (EC), milking time and peak flow.					
29785910	10	66	theme	electrical	1482:1491	arg1	conductivity					1493:1504	electrical conductivity	1482:1504	electrical conductivity (EC)	1482:1509	The following data were analysed: milk volume, protein concentration, lactose concentration, electrical conductivity (EC), milking time and peak flow.					
29785910	2	67	theme	features	339:346	arg1	mining					312:317	large-scale mining	300:317	large-scale mining of milk composition features by pattern recognition models	300:376	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	9	68	theme	248	1366:1368	arg1	records					1370:1376	346 248 records	1362:1376	346 248 records in total	1362:1385	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29785910	6	69	theme	Test-day	853:860	arg1	Cell					870:873	Test-day Somatic Cell	853:873	Test-day Somatic Cell Count (SCC)	853:885	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	10	70	theme	milking	1512:1518	arg1	time					1520:1523	milking time	1512:1523	milking time	1512:1523	The following data were analysed: milk volume, protein concentration, lactose concentration, electrical conductivity (EC), milking time and peak flow.					
29785910	5	71	theme	great	835:839	arg1	importance					841:850	great importance	835:850	great importance	835:850	Developing new techniques to diagnose SCM in its early stages improves herd health and is of great importance.					
29785910	8	72	theme	additional	1137:1146	arg1	indicators					1158:1167	additional efficient indicators	1137:1167	additional efficient indicators	1137:1167	Consequently, there would be great benefit to identifying additional efficient indicators from large-scale and longitudinal studies.					
29785910	14	73	from	concentration	1961:1973	arg1	milk					1989:1992	milk	1989:1992	milk	1989:1992	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	0	74	theme	composition	115:125	arg1	features					127:134	milk composition features	110:134	milk composition features	110:134	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	14	75	theme	lactose	1978:1984	arg1	concentration					1961:1973	the concentration	1957:1973	the concentration of lactose in milk	1957:1992	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	14	75	theme	lactose	1978:1984	arg1	indicator					2018:2026	a strong indicator	2009:2026	a strong indicator of SCM	2009:2033	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	2	76	theme	composition	327:337	arg1	features					339:346	milk composition features	322:346	milk composition features	322:346	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	15	77	theme	predictive	2173:2182	arg1	pattern					2184:2190	a predictive pattern	2171:2190	a predictive pattern for early SCM detection	2171:2214	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC) may produce a predictive pattern for early SCM detection.					
29785910	3	78	theme	various	576:582	arg1	parameters					600:609	various milk production parameters	576:609	various milk production parameters	576:609	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	1	79	theme	Sub-clinical	211:222	arg1	SCM					234:236	SCM	234:236	SCM	234:236	Sub-clinical mastitis (SCM) affects milk composition.					
29785910	1	79	theme	Sub-clinical	211:222	arg1	mastitis					224:231	Sub-clinical mastitis	211:231	Sub-clinical mastitis (SCM)	211:237	Sub-clinical mastitis (SCM) affects milk composition.					
29785910	8	80	theme	large-scale	1174:1184	arg1	studies					1203:1209	large-scale and longitudinal studies	1174:1209	large-scale and longitudinal studies	1174:1209	Consequently, there would be great benefit to identifying additional efficient indicators from large-scale and longitudinal studies.					
29785910	6	81	theme	common	899:904	arg1	Count					875:879	Test-day Somatic Cell Count	853:879	Test-day Somatic Cell Count (SCC)	853:885	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	6	81	theme	common	899:904	arg1	indicator					906:914	the most common indicator	890:914	the most common indicator of SCM and the primary mastitis surveillance approach worldwide	890:978	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	8	82	theme	longitudinal	1190:1201	arg1	studies					1203:1209	large-scale and longitudinal studies	1174:1209	large-scale and longitudinal studies	1174:1209	Consequently, there would be great benefit to identifying additional efficient indicators from large-scale and longitudinal studies.					
29785910	4	83	theme	substantial	692:702	arg1	loss					713:716	substantial economic loss	692:716	substantial economic loss for the dairy industry	692:739	SCM is the most prevalent disease of dairy cattle, causing substantial economic loss for the dairy industry.					
29785910	11	84	theme	European	1635:1642	arg1	cells/ml					1653:1660	European ≥200 000 cells/ml	1635:1660	European ≥200 000 cells/ml	1635:1660	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	0	85	theme	dairy	62:66	arg1	cattle					68:73	dairy cattle	62:73	dairy cattle	62:73	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	3	86	theme	production	589:598	arg1	parameters					600:609	various milk production parameters	576:609	various milk production parameters	576:609	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	14	87	used	used	2001:2004	arg2	indicator					2018:2026	a strong indicator	2009:2026	a strong indicator of SCM	2009:2033	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	14	87	used	used	2001:2004	arg2	concentration					1961:1973	the concentration	1957:1973	the concentration of lactose in milk	1957:1992	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	5	88	from	SCM	780:782	arg1	stages					797:802	its early stages	787:802	its early stages	787:802	Developing new techniques to diagnose SCM in its early stages improves herd health and is of great importance.					
29785910	6	89	theme	surveillance	948:959	arg1	approach					961:968	the primary mastitis surveillance approach	927:968	the primary mastitis surveillance approach	927:968	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	4	90	theme	dairy	670:674	arg1	cattle					676:681	dairy cattle	670:681	dairy cattle	670:681	SCM is the most prevalent disease of dairy cattle, causing substantial economic loss for the dairy industry.					
29785910	0	91	theme	weighting	88:96	arg1	analysis					98:105	attribute weighting analysis	78:105	attribute weighting analysis of milk composition features	78:134	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	6	92	theme	primary	931:937	arg1	approach					961:968	the primary mastitis surveillance approach	927:968	the primary mastitis surveillance approach	927:968	Test-day Somatic Cell Count (SCC) is the most common indicator of SCM and the primary mastitis surveillance approach worldwide.					
29785910	0	93	from	mastitis	50:57	arg1	cattle					68:73	dairy cattle	62:73	dairy cattle	62:73	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	11	94	theme	000	1649:1651	arg1	cells/ml					1653:1660	European ≥200 000 cells/ml	1635:1660	European ≥200 000 cells/ml	1635:1660	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	15	95	theme	SCM	2202:2204	arg1	detection					2206:2214	early SCM detection	2196:2214	early SCM detection	2196:2214	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC) may produce a predictive pattern for early SCM detection.					
29785910	14	96	theme	weighted	1922:1929	arg1	modelling					1931:1939	attribute weighted modelling	1912:1939	attribute weighted modelling	1912:1939	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	0	97	theme	large-scale	2:12	arg1	study					14:18	A large-scale study	0:18	A large-scale study of indicators of sub-clinical mastitis in dairy cattle	0:73	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	5	98	theme	early	791:795	arg1	stages					797:802	its early stages	787:802	its early stages	787:802	Developing new techniques to diagnose SCM in its early stages improves herd health and is of great importance.					
29785910	12	99	theme	Weighting	1731:1739	arg1	AWM					1753:1755	AWM	1753:1755	AWM	1753:1755	At first, 10 different Attribute Weighting Algorithms (AWM) were applied to the data.					
29785910	12	99	theme	Weighting	1731:1739	arg1	Algorithms					1741:1750	10 different Attribute Weighting Algorithms	1708:1750	10 different Attribute Weighting Algorithms (AWM)	1708:1756	At first, 10 different Attribute Weighting Algorithms (AWM) were applied to the data.					
29785910	2	100	theme	SCM	414:416	arg1	features					446:453	SCM within the milk composition features	414:453	SCM within the milk composition features	414:453	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	15	101	theme	systems	2079:2085	arg1	development					2040:2050	The development	2036:2050	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC)	2036:2157	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC) may produce a predictive pattern for early SCM detection.					
29785910	9	102	dep	day	1277:1279	arg1	twice					1267:1271	twice	1267:1271	twice	1267:1271	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29785910	10	103	theme	peak	1529:1532	arg1	flow					1534:1537	peak flow	1529:1537	peak flow	1529:1537	The following data were analysed: milk volume, protein concentration, lactose concentration, electrical conductivity (EC), milking time and peak flow.					
29785910	9	104	theme	electronic	1340:1349	arg1	equipment					1351:1359	in-line electronic equipment	1332:1359	in-line electronic equipment (346 248 records in total)	1332:1386	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29785910	0	105	theme	mastitis	50:57	arg1	indicators					23:32	indicators	23:32	indicators of sub-clinical mastitis in dairy cattle	23:73	A large-scale study of indicators of sub-clinical mastitis in dairy cattle by attribute weighting analysis of milk composition features: highlighting the predictive power of lactose and electrical conductivity.					
29785910	9	106	theme	months	1300:1305	arg1	period					1288:1293	a period	1286:1293	a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total)	1286:1386	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29785910	14	107	theme	SCM	2031:2033	arg1	concentration					1961:1973	the concentration	1957:1973	the concentration of lactose in milk	1957:1992	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	14	107	theme	SCM	2031:2033	arg1	indicator					2018:2026	a strong indicator	2009:2026	a strong indicator of SCM	2009:2033	For the first time, using attribute weighted modelling, we showed that the concentration of lactose in milk can be used as a strong indicator of SCM.					
29785910	11	108	theme	≥	1615:1615	arg1	cells/ml					1625:1632	Australian ≥ 250 000 cells/ml	1604:1632	Australian ≥ 250 000 cells/ml	1604:1632	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	3	109	theme	mining	480:485	arg1	algorithms					487:496	data mining algorithms	475:496	data mining algorithms	475:496	To this end, using data mining algorithms, we conducted a large-scale and longitudinal study to evaluate the ability of various milk production parameters as indicators of SCM.					
29785910	12	110	theme	different	1711:1719	arg1	AWM					1753:1755	AWM	1753:1755	AWM	1753:1755	At first, 10 different Attribute Weighting Algorithms (AWM) were applied to the data.					
29785910	12	110	theme	different	1711:1719	arg1	Algorithms					1741:1750	10 different Attribute Weighting Algorithms	1708:1750	10 different Attribute Weighting Algorithms (AWM)	1708:1756	At first, 10 different Attribute Weighting Algorithms (AWM) were applied to the data.					
29785910	10	111	theme	milk	1423:1426	arg1	volume					1428:1433	milk volume	1423:1433	milk volume	1423:1433	The following data were analysed: milk volume, protein concentration, lactose concentration, electrical conductivity (EC), milking time and peak flow.					
29785910	15	112	theme	lactose	2129:2135	arg1	concentration					2137:2149	lactose concentration	2129:2149	lactose concentration	2129:2149	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC) may produce a predictive pattern for early SCM detection.					
29785910	15	113	dep	variables	2110:2118	arg1	such					2121:2124	such	2121:2124	such	2121:2124	The development of machine-learning expert systems using two or more milk variables (such as lactose concentration and EC) may produce a predictive pattern for early SCM detection.					
29785910	10	114	theme	lactose	1459:1465	arg1	concentration					1467:1479	lactose concentration	1459:1479	lactose concentration	1459:1479	The following data were analysed: milk volume, protein concentration, lactose concentration, electrical conductivity (EC), milking time and peak flow.					
29785910	11	115	theme	SCC	1546:1548	arg1	cut-offs					1550:1557	Three SCC cut-offs	1540:1557	Three SCC cut-offs	1540:1557	Three SCC cut-offs were used to estimate the prevalence of SCM: Australian ≥ 250 000 cells/ml, European ≥200 000 cells/ml and New Zealand ≥ 150 000 cells/ml.					
29785910	2	116	theme	recognition	359:369	arg1	models					371:376	pattern recognition models	351:376	pattern recognition models	351:376	In this study, we hypothesise that large-scale mining of milk composition features by pattern recognition models can identify the best predictors of SCM within the milk composition features.					
29785910	9	117	theme	in-line	1332:1338	arg1	equipment					1351:1359	in-line electronic equipment	1332:1359	in-line electronic equipment (346 248 records in total)	1332:1386	With this intent, data was collected at every milking (twice per day) for a period of 2 months from a single farm using in-line electronic equipment (346 248 records in total).					
29897525	10	0	theme	Dietary	1437:1443	arg1	treatment					1445:1453	Dietary treatment	1437:1453	Dietary treatment	1437:1453	Dietary treatment resulted in non-significant effect on BWG, but Sal addition resulted in increased FI.					
29897525	9	1	theme	significant	1394:1404	arg1	reduction					1406:1414	a significant reduction	1392:1414	a significant reduction in 0-28 d BW and FI	1392:1434	The coccidiosis challenge main effect resulted in a significant reduction in 0-28 d BW and FI.					
29897525	3	2	from	groups	436:441	arg1	arrangement					497:507	a 2 × 4 factorial arrangement	479:507	a 2 × 4 factorial arrangement	479:507	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	1	3	theme	multiple	175:182	arg1	methods					195:201	multiple prevention methods	175:201	multiple prevention methods being explored to control its impact	175:238	Coccidiosis is a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact.					
29897525	4	4	theme	Eimeria	762:768	arg1	inoculum					770:777	a mixed Eimeria inoculum	754:777	a mixed Eimeria inoculum	754:777	On d15, birds in the challenge groups received a mixed Eimeria inoculum.					
29897525	5	5	theme	feed	870:873	arg1	intake					875:880	feed intake	870:880	feed intake (FI)	870:885	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	5	5	theme	feed	870:873	arg1	FI					883:884	FI	883:884	FI	883:884	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	2	6	theme	β-glucans	285:293	arg1	effects					274:280	the feeding effects	262:280	the feeding effects of β-glucans on performance and responses of chickens	262:334	This study evaluated the feeding effects of β-glucans on performance and responses of chickens during a coccidiosis challenge.					
29897525	9	7	from	reduction	1406:1414	arg1	FI					1433:1434	FI	1433:1434	FI	1433:1434	The coccidiosis challenge main effect resulted in a significant reduction in 0-28 d BW and FI.					
29897525	9	7	from	reduction	1406:1414	arg1	BW					1426:1427	0-28 d BW	1419:1427	0-28 d BW	1419:1427	The coccidiosis challenge main effect resulted in a significant reduction in 0-28 d BW and FI.					
29897525	11	8	theme	challenged	1755:1764	arg1	groups					1779:1784	the challenged Sal and BGZn groups	1751:1784	the challenged Sal and BGZn groups	1751:1784	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	8	9	theme	dietary	1194:1200	arg1	treatments					1202:1211	the dietary treatments	1190:1211	the dietary treatments	1190:1211	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	3	10	theme	Algamune	651:658	arg1	50 Zn					660:664	100 g/MT Algamune 50 Zn	642:664	100 g/MT Algamune 50 Zn	642:664	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	10	theme	Algamune	651:658	arg1	control					626:632	control	626:632	control	626:632	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	12	11	theme	composition	1792:1802	arg1	analysis					1804:1811	Body composition analysis	1787:1811	Body composition analysis at d28	1787:1818	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	1	12	theme	costly	121:126	arg1	disease					138:144	a costly parasitic disease	119:144	a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact	119:238	Coccidiosis is a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact.					
29897525	1	12	theme	costly	121:126	arg1	Coccidiosis					104:114	Coccidiosis	104:114	Coccidiosis	104:114	Coccidiosis is a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact.					
29897525	8	13	theme	infected	1220:1227	arg1	groups					1229:1234	the infected groups	1216:1234	the infected groups	1216:1234	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	5	14	theme	conversion	897:906	arg1	ratios					908:913	feed conversion ratios	892:913	feed conversion ratios (FCR)	892:919	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	5	14	theme	conversion	897:906	arg1	FCR					916:918	FCR	916:918	FCR	916:918	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	11	15	theme	other	1691:1695	arg1	groups					1708:1713	the other challenged groups	1687:1713	the other challenged groups	1687:1713	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	0	16	theme	chickens	63:70	arg1	performance					27:37	performance	27:37	performance	27:37	The impact of β-glucans on performance and response of broiler chickens during a coccidiosis challenge.					
29897525	0	16	theme	chickens	63:70	arg1	response					43:50	response	43:50	response	43:50	The impact of β-glucans on performance and response of broiler chickens during a coccidiosis challenge.					
29897525	4	17	from	birds	715:719	arg1	groups					738:743	the challenge groups	724:743	the challenge groups	724:743	On d15, birds in the challenge groups received a mixed Eimeria inoculum.					
29897525	12	18	contain	had	1933:1935	arg1	birds					1927:1931	the β-glucans and Sal birds	1905:1931	the β-glucans and Sal birds	1905:1931	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	12	18	contain	had	1933:1935	arg2	percent					1947:1953	lower fat percent	1937:1953	lower fat percent	1937:1953	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	8	19	theme	duodenal	1249:1256	arg1	scores					1268:1273	reduced duodenal and cecal scores	1241:1273	reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively	1241:1339	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	3	20	theme	%	686:686	arg1	Salinomycin					688:698	0.01% Salinomycin	682:698	0.01% Salinomycin (Sal)	682:704	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	20	theme	%	686:686	arg1	groups					436:441	1 of 8 treatment groups	419:441	1 of 8 treatment groups (8 replicate pens; 20 birds/pen)	419:474	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	20	theme	%	686:686	arg1	birds					554:558	non-infected and Eimeria-infected birds	520:558	non-infected and Eimeria-infected birds fed for 28 d a control diet	520:586	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	20	theme	%	686:686	arg1	Sal					701:703	Sal	701:703	Sal	701:703	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	1	21	with	disease	138:144	arg1	methods					195:201	multiple prevention methods	175:201	multiple prevention methods being explored to control its impact	175:238	Coccidiosis is a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact.					
29897525	8	22	theme	cecal	1262:1266	arg1	scores					1268:1273	reduced duodenal and cecal scores	1241:1273	reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively	1241:1339	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	11	23	from	FCR	1603:1605	arg1	birds					1633:1637	the Eimeria-challenged birds	1610:1637	the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups	1610:1713	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	12	24	theme	lower	1937:1941	arg1	percent					1947:1953	lower fat percent	1937:1953	lower fat percent	1937:1953	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	11	25	theme	Eimeria-challenged	1614:1631	arg1	birds					1633:1637	the Eimeria-challenged birds	1610:1637	the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups	1610:1713	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	7	26	theme	LS-MEANS	1079:1086	arg1	statement					1088:1096	the LS-MEANS statement	1075:1096	the LS-MEANS statement with significance reported at P ≤ 0.05	1075:1135	Performance data were subjected to ANOVA and differences were established using the LS-MEANS statement with significance reported at P ≤ 0.05.					
29897525	3	27	theme	male	377:380	arg1	n					392:392	n = 1280	392:399	n = 1280	392:399	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	27	theme	male	377:380	arg1	broilers					382:389	Cobb 500 male broilers	368:389	Cobb 500 male broilers (n = 1280)	368:400	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	0	28	theme	broiler	55:61	arg1	chickens					63:70	broiler chickens	55:70	broiler chickens	55:70	The impact of β-glucans on performance and response of broiler chickens during a coccidiosis challenge.					
29897525	3	29	theme	Cobb	368:371	arg1	n					392:392	n = 1280	392:399	n = 1280	392:399	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	29	theme	Cobb	368:371	arg1	broilers					382:389	Cobb 500 male broilers	368:389	Cobb 500 male broilers (n = 1280)	368:400	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	8	30	theme	Sal	1282:1284	arg1	group					1286:1290	the Sal group	1278:1290	the Sal group compared to the BGZn and BG groups, respectively	1278:1339	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	10	31	theme	non-significant	1467:1481	arg1	effect					1483:1488	non-significant effect	1467:1488	non-significant effect on BWG	1467:1495	Dietary treatment resulted in non-significant effect on BWG, but Sal addition resulted in increased FI.					
29897525	3	32	theme	=	394:394	arg1	n					392:392	n = 1280	392:399	n = 1280	392:399	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	32	theme	=	394:394	arg1	broilers					382:389	Cobb 500 male broilers	368:389	Cobb 500 male broilers (n = 1280)	368:400	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	33	theme	control	575:581	arg1	diet					583:586	a control diet	573:586	a control diet	573:586	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	12	34	theme	tissue	1882:1887	arg1	contents					1889:1896	both fat and lean tissue contents	1864:1896	both fat and lean tissue contents	1864:1896	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	2	35	from	effects	274:280	arg1	responses					314:322	responses	314:322	responses	314:322	This study evaluated the feeding effects of β-glucans on performance and responses of chickens during a coccidiosis challenge.					
29897525	2	35	from	effects	274:280	arg1	performance					298:308	performance	298:308	performance	298:308	This study evaluated the feeding effects of β-glucans on performance and responses of chickens during a coccidiosis challenge.					
29897525	5	36	theme	body	846:849	arg1	BWG					864:866	BWG	864:866	BWG	864:866	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	5	36	theme	body	846:849	arg1	gain					858:861	body weight gain	846:861	body weight gain (BWG)	846:867	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	8	37	theme	minor	1149:1153	arg1	differences					1155:1165	minor differences	1149:1165	minor differences in lesion scores among the dietary treatments	1149:1211	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	11	38	theme	challenge	1562:1570	arg1	interaction					1572:1582	A significant diet X challenge interaction	1541:1582	A significant diet X challenge interaction	1541:1582	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	11	39	theme	diet	1555:1558	arg1	interaction					1572:1582	A significant diet X challenge interaction	1541:1582	A significant diet X challenge interaction	1541:1582	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	9	40	theme	coccidiosis	1346:1356	arg1	effect					1373:1378	The coccidiosis challenge main effect	1342:1378	The coccidiosis challenge main effect	1342:1378	The coccidiosis challenge main effect resulted in a significant reduction in 0-28 d BW and FI.					
29897525	11	41	theme	higher	1596:1601	arg1	FCR					1603:1605	higher FCR	1596:1605	higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups	1596:1713	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	9	42	theme	main	1368:1371	arg1	effect					1373:1378	The coccidiosis challenge main effect	1342:1378	The coccidiosis challenge main effect	1342:1378	The coccidiosis challenge main effect resulted in a significant reduction in 0-28 d BW and FI.					
29897525	7	43	theme	Performance	995:1005	arg1	data					1007:1010	Performance data	995:1010	Performance data	995:1010	Performance data were subjected to ANOVA and differences were established using the LS-MEANS statement with significance reported at P ≤ 0.05.					
29897525	2	44	theme	coccidiosis	345:355	arg1	challenge					357:365	a coccidiosis challenge	343:365	a coccidiosis challenge	343:365	This study evaluated the feeding effects of β-glucans on performance and responses of chickens during a coccidiosis challenge.					
29897525	3	45	theme	factorial	487:495	arg1	arrangement					497:507	a 2 × 4 factorial arrangement	479:507	a 2 × 4 factorial arrangement	479:507	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	2	46	theme	feeding	266:272	arg1	effects					274:280	the feeding effects	262:280	the feeding effects of β-glucans on performance and responses of chickens	262:334	This study evaluated the feeding effects of β-glucans on performance and responses of chickens during a coccidiosis challenge.					
29897525	1	47	theme	poultry	153:159	arg1	industry					161:168	the poultry industry	149:168	the poultry industry	149:168	Coccidiosis is a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact.					
29897525	10	48	theme	Sal	1502:1504	arg1	addition					1506:1513	Sal addition	1502:1513	Sal addition	1502:1513	Dietary treatment resulted in non-significant effect on BWG, but Sal addition resulted in increased FI.					
29897525	6	49	theme	Lesion	922:927	arg1	scores					929:934	Lesion scores	922:934	Lesion scores	922:934	Lesion scores were assessed 6 d post infection (d21) on 3 birds per pen.					
29897525	11	50	from	BGZn	1665:1668	arg1	comparison					1673:1682	comparison	1673:1682	comparison to the other challenged groups	1673:1713	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	12	51	theme	Eimeria	1838:1844	arg1	challenge					1846:1854	the Eimeria challenge	1834:1854	the Eimeria challenge	1834:1854	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	3	52	theme	100 g/MT	642:649	arg1	50 Zn					660:664	100 g/MT Algamune 50 Zn	642:664	100 g/MT Algamune 50 Zn	642:664	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	52	theme	100 g/MT	642:649	arg1	control					626:632	control	626:632	control	626:632	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	53	theme	×	483:483	arg1	arrangement					497:507	a 2 × 4 factorial arrangement	479:507	a 2 × 4 factorial arrangement	479:507	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	1	54	theme	prevention	184:193	arg1	methods					195:201	multiple prevention methods	175:201	multiple prevention methods being explored to control its impact	175:238	Coccidiosis is a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact.					
29897525	11	55	theme	BGZn	1774:1777	arg1	groups					1779:1784	the challenged Sal and BGZn groups	1751:1784	the challenged Sal and BGZn groups	1751:1784	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	3	56	theme	non-infected	520:531	arg1	birds					554:558	non-infected and Eimeria-infected birds	520:558	non-infected and Eimeria-infected birds fed for 28 d a control diet	520:586	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	8	57	from	differences	1155:1165	arg1	scores					1177:1182	lesion scores	1170:1182	lesion scores	1170:1182	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	11	58	theme	Sal	1766:1768	arg1	groups					1779:1784	the challenged Sal and BGZn groups	1751:1784	the challenged Sal and BGZn groups	1751:1784	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	11	59	theme	significant	1543:1553	arg1	interaction					1572:1582	A significant diet X challenge interaction	1541:1582	A significant diet X challenge interaction	1541:1582	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	4	60	theme	challenge	728:736	arg1	groups					738:743	the challenge groups	724:743	the challenge groups	724:743	On d15, birds in the challenge groups received a mixed Eimeria inoculum.					
29897525	11	61	from	Sal	1657:1659	arg1	comparison					1673:1682	comparison	1673:1682	comparison to the other challenged groups	1673:1713	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	11	62	theme	challenged	1697:1706	arg1	groups					1708:1713	the other challenged groups	1687:1713	the other challenged groups	1687:1713	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	11	63	theme	reduced	1730:1736	arg1	mortality					1738:1746	reduced mortality	1730:1746	reduced mortality in the challenged Sal and BGZn groups	1730:1784	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	12	64	theme	Body	1787:1790	arg1	analysis					1804:1811	Body composition analysis	1787:1811	Body composition analysis at d28	1787:1818	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	3	65	theme	0.01	682:685	arg1	%					686:686	%	686:686	%	686:686	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	7	66	with	statement	1088:1096	arg1	significance					1103:1114	significance	1103:1114	significance reported at P ≤ 0.05	1103:1135	Performance data were subjected to ANOVA and differences were established using the LS-MEANS statement with significance reported at P ≤ 0.05.					
29897525	3	67	theme	Eimeria-infected	537:552	arg1	birds					554:558	non-infected and Eimeria-infected birds	520:558	non-infected and Eimeria-infected birds fed for 28 d a control diet	520:586	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	12	68	theme	fat	1869:1871	arg1	contents					1889:1896	both fat and lean tissue contents	1864:1896	both fat and lean tissue contents	1864:1896	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	1	69	theme	parasitic	128:136	arg1	disease					138:144	a costly parasitic disease	119:144	a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact	119:238	Coccidiosis is a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact.					
29897525	1	69	theme	parasitic	128:136	arg1	Coccidiosis					104:114	Coccidiosis	104:114	Coccidiosis	104:114	Coccidiosis is a costly parasitic disease to the poultry industry with multiple prevention methods being explored to control its impact.					
29897525	10	70	from	effect	1483:1488	arg1	BWG					1493:1495	BWG	1493:1495	BWG	1493:1495	Dietary treatment resulted in non-significant effect on BWG, but Sal addition resulted in increased FI.					
29897525	8	71	theme	reduced	1241:1247	arg1	scores					1268:1273	reduced duodenal and cecal scores	1241:1273	reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively	1241:1339	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	4	72	theme	mixed	756:760	arg1	inoculum					770:777	a mixed Eimeria inoculum	754:777	a mixed Eimeria inoculum	754:777	On d15, birds in the challenge groups received a mixed Eimeria inoculum.					
29897525	0	73	from	impact	4:9	arg1	performance					27:37	performance	27:37	performance	27:37	The impact of β-glucans on performance and response of broiler chickens during a coccidiosis challenge.					
29897525	0	73	from	impact	4:9	arg1	response					43:50	response	43:50	response	43:50	The impact of β-glucans on performance and response of broiler chickens during a coccidiosis challenge.					
29897525	0	74	theme	coccidiosis	81:91	arg1	challenge					93:101	a coccidiosis challenge	79:101	a coccidiosis challenge	79:101	The impact of β-glucans on performance and response of broiler chickens during a coccidiosis challenge.					
29897525	5	75	theme	pen	824:826	arg1	basis					828:832	a per pen basis	818:832	a per pen basis	818:832	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	3	76	dep	groups	436:441	arg1	pens					456:459	8 replicate pens	444:459	8 replicate pens; 20 birds/pen	444:473	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	12	77	theme	Sal	1923:1925	arg1	birds					1927:1931	the β-glucans and Sal birds	1905:1931	the β-glucans and Sal birds	1905:1931	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	12	78	theme	control	1960:1966	arg1	birds					1968:1972	control birds	1960:1972	control birds	1960:1972	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	5	79	theme	feed	892:895	arg1	ratios					908:913	feed conversion ratios	892:913	feed conversion ratios (FCR)	892:919	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	5	79	theme	feed	892:895	arg1	FCR					916:918	FCR	916:918	FCR	916:918	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	12	80	from	d28	1816:1818	arg1	analysis					1804:1811	Body composition analysis	1787:1811	Body composition analysis at d28	1787:1818	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	3	81	dep	pens	456:459	arg1	replicate					446:454	replicate	446:454	replicate	446:454	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	81	dep	pens	456:459	arg1	birds/pen					465:473	20 birds/pen	462:473	8 replicate pens; 20 birds/pen	444:473	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	12	82	theme	fat	1943:1945	arg1	percent					1947:1953	lower fat percent	1937:1953	lower fat percent	1937:1953	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	0	83	theme	β-glucans	14:22	arg1	impact					4:9	The impact	0:9	The impact of β-glucans on performance and response of broiler chickens during a coccidiosis challenge.	0:102	The impact of β-glucans on performance and response of broiler chickens during a coccidiosis challenge.					
29897525	10	84	theme	increased	1527:1535	arg1	FI					1537:1538	increased FI	1527:1538	increased FI	1527:1538	Dietary treatment resulted in non-significant effect on BWG, but Sal addition resulted in increased FI.					
29897525	12	85	theme	β-glucans	1909:1917	arg1	birds					1927:1931	the β-glucans and Sal birds	1905:1931	the β-glucans and Sal birds	1905:1931	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	3	86	theme	treatment	426:434	arg1	control					626:632	control	626:632	control	626:632	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	86	theme	treatment	426:434	arg1	groups					436:441	1 of 8 treatment groups	419:441	1 of 8 treatment groups (8 replicate pens; 20 birds/pen)	419:474	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	86	theme	treatment	426:434	arg1	control					672:678	control	672:678	control	672:678	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	86	theme	treatment	426:434	arg1	Salinomycin					688:698	0.01% Salinomycin	682:698	0.01% Salinomycin (Sal)	682:704	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	86	theme	treatment	426:434	arg1	birds					554:558	non-infected and Eimeria-infected birds	520:558	non-infected and Eimeria-infected birds fed for 28 d a control diet	520:586	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	86	theme	treatment	426:434	arg1	control					589:595	control	589:595	control	589:595	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	86	theme	treatment	426:434	arg1	BG					599:600	BG	599:600	BG	599:600	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	8	87	theme	BGZn	1308:1311	arg1	groups					1320:1325	the BGZn and BG groups	1304:1325	the BGZn and BG groups	1304:1325	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	12	88	theme	lean	1877:1880	arg1	contents					1889:1896	both fat and lean tissue contents	1864:1896	both fat and lean tissue contents	1864:1896	Body composition analysis at d28 revealed that the Eimeria challenge reduced both fat and lean tissue contents, where the β-glucans and Sal birds had lower fat percent than control birds.					
29897525	8	89	from	scores	1268:1273	arg1	group					1286:1290	the Sal group	1278:1290	the Sal group compared to the BGZn and BG groups, respectively	1278:1339	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	5	90	theme	weight	851:856	arg1	BWG					864:866	BWG	864:866	BWG	864:866	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	5	90	theme	weight	851:856	arg1	gain					858:861	body weight gain	846:861	body weight gain (BWG)	846:867	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	8	91	theme	BG	1317:1318	arg1	groups					1320:1325	the BGZn and BG groups	1304:1325	the BGZn and BG groups	1304:1325	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
29897525	2	92	theme	chickens	327:334	arg1	responses					314:322	responses	314:322	responses	314:322	This study evaluated the feeding effects of β-glucans on performance and responses of chickens during a coccidiosis challenge.					
29897525	2	92	theme	chickens	327:334	arg1	performance					298:308	performance	298:308	performance	298:308	This study evaluated the feeding effects of β-glucans on performance and responses of chickens during a coccidiosis challenge.					
29897525	9	93	theme	d	1424:1424	arg1	BW					1426:1427	0-28 d BW	1419:1427	0-28 d BW	1419:1427	The coccidiosis challenge main effect resulted in a significant reduction in 0-28 d BW and FI.					
29897525	5	94	theme	per	820:822	arg1	basis					828:832	a per pen basis	818:832	a per pen basis	818:832	Birds and feed were weighed weekly on a per pen basis to evaluate body weight gain (BWG), feed intake (FI), and feed conversion ratios (FCR).					
29897525	11	95	from	mortality	1738:1746	arg1	groups					1779:1784	the challenged Sal and BGZn groups	1751:1784	the challenged Sal and BGZn groups	1751:1784	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	11	96	theme	X	1560:1560	arg1	interaction					1572:1582	A significant diet X challenge interaction	1541:1582	A significant diet X challenge interaction	1541:1582	A significant diet X challenge interaction resulted in higher FCR in the Eimeria-challenged birds supplemented with Sal and BGZn in comparison to the other challenged groups, likely due to reduced mortality in the challenged Sal and BGZn groups.					
29897525	9	97	theme	challenge	1358:1366	arg1	effect					1373:1378	The coccidiosis challenge main effect	1342:1378	The coccidiosis challenge main effect	1342:1378	The coccidiosis challenge main effect resulted in a significant reduction in 0-28 d BW and FI.					
29897525	3	98	theme	150 g/MT	603:610	arg1	Algamune					612:619	150 g/MT Algamune 50	603:622	150 g/MT Algamune 50	603:622	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	3	98	theme	150 g/MT	603:610	arg1	control					589:595	control	589:595	control	589:595	Cobb 500 male broilers (n = 1280) were assigned to 1 of 8 treatment groups (8 replicate pens; 20 birds/pen) in a 2 × 4 factorial arrangement, including non-infected and Eimeria-infected birds fed for 28 d a control diet, control + BG (150 g/MT Algamune 50), control + BGZn (100 g/MT Algamune 50 Zn), and control + 0.01% Salinomycin (Sal).					
29897525	8	99	theme	lesion	1170:1175	arg1	scores					1177:1182	lesion scores	1170:1182	lesion scores	1170:1182	There were minor differences in lesion scores among the dietary treatments in the infected groups with reduced duodenal and cecal scores in the Sal group compared to the BGZn and BG groups, respectively.					
30075778	9	0	theme	Lipid	1545:1549	arg1	tails					1551:1555	Lipid tails	1545:1555	Lipid tails	1545:1555	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	6	1	theme	active	1149:1154	arg1	transport					1156:1164	both H+- and Na+-coupled melibiose active transport	1114:1164	both H+- and Na+-coupled melibiose active transport to different extents	1114:1185	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	2	2	theme	melibiose	304:312	arg1	MelB					266:269	Salmonella typhimurium MelB	243:269	Salmonella typhimurium MelB (MelBSt)	243:278	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	2	2	theme	melibiose	304:312	arg1	translocation					314:326	a symporter coupling melibiose translocation	283:326	a symporter coupling melibiose translocation	283:326	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	9	3	theme	of MelBSt	1654:1662	arg1	stability					1627:1635	the stability	1623:1635	the stability of the structure of MelBSt	1623:1662	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	6	4	theme	Na+-coupled	1127:1137	arg1	transport					1156:1164	both H+- and Na+-coupled melibiose active transport	1114:1164	both H+- and Na+-coupled melibiose active transport to different extents	1114:1185	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	1	5	theme	membrane-embedded	211:227	arg1	transporters					229:240	membrane-embedded transporters	211:240	membrane-embedded transporters	211:240	BACKGROUND Membrane lipids play critical roles in the structure and function of membrane-embedded transporters.					
30075778	6	6	theme	cardiolipin	992:1002	arg1	strains					1044:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	6	7	theme	H+-	1119:1121	arg1	transport					1156:1164	both H+- and Na+-coupled melibiose active transport	1114:1164	both H+- and Na+-coupled melibiose active transport to different extents	1114:1185	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	5	8	theme	backbones	852:860	arg1	presence					815:822	the presence	811:822	the presence of lipid tails and glycerol backbones that co-purified with MelBSt	811:889	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	6	9	theme	PE-deficient	978:989	arg1	strains					1044:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	8	10	theme	headgroup	1414:1422	arg1	species					1424:1430	the headgroup species	1410:1430	the headgroup species	1410:1430	Remarkably, the delipidated MelBSt with only 2-3 bound lipids, regardless of the headgroup species, also exhibits unchanged melting temperature values as shown by circular dichroism spectroscopy.					
30075778	4	11	theme	high	712:715	arg1	affinities					717:726	high affinities	712:726	high affinities	712:726	RESULTS Lipidomic analysis and thin-layer chromatography (TLC) experiments reveal that at least one phosphatidylethanolamine (PE) and one phosphatidylglycerol (PG) molecule associate with MelBSt at high affinities.					
30075778	10	12	theme	important	1718:1726	arg1	roles					1728:1732	important roles	1718:1732	important roles	1718:1732	(2) The headgroups of PE and PG, but not of CL, play important roles in melibiose transport; however, lipid headgroups do not modulate the folding and stability of MelBSt.					
30075778	6	13	theme	lipid-engineered	942:957	arg1	strains					1044:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	6	13	theme	lipid-engineered	942:957	arg1	strains					959:965	lipid-engineered strains	942:965	lipid-engineered strains	942:965	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	10	14	dep	play	1713:1716	arg1	2					1666:1666	2	1666:1666	2	1666:1666	(2) The headgroups of PE and PG, but not of CL, play important roles in melibiose transport; however, lipid headgroups do not modulate the folding and stability of MelBSt.					
30075778	2	15	theme	symporter	285:293	arg1	MelB					266:269	Salmonella typhimurium MelB	243:269	Salmonella typhimurium MelB (MelBSt)	243:278	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	2	15	theme	symporter	285:293	arg1	translocation					314:326	a symporter coupling melibiose translocation	283:326	a symporter coupling melibiose translocation	283:326	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	1	16	theme	critical	163:170	arg1	roles					172:176	critical roles	163:176	critical roles	163:176	BACKGROUND Membrane lipids play critical roles in the structure and function of membrane-embedded transporters.					
30075778	8	17	theme	melting	1457:1463	arg1	values					1477:1482	unchanged melting temperature values	1447:1482	unchanged melting temperature values	1447:1482	Remarkably, the delipidated MelBSt with only 2-3 bound lipids, regardless of the headgroup species, also exhibits unchanged melting temperature values as shown by circular dichroism spectroscopy.					
30075778	7	18	theme	co-substrate	1215:1226	arg1	binding					1228:1234	the co-substrate binding	1211:1234	the co-substrate binding (melibiose or Na+)	1211:1253	Interestingly, neither the co-substrate binding (melibiose or Na+) nor MelBSt folding and stability are affected by changing lipid compositions.					
30075778	2	19	with	translocation	314:326	arg1	cation					335:340	a cation	333:340	a cation (Na+, Li+, or H+)	333:358	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	5	20	theme	spectroscopy	776:787	arg1	experiments					789:799	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments	729:799	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments	729:799	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	1	21	theme	BACKGROUND	131:140	arg1	lipids					151:156	BACKGROUND Membrane lipids	131:156	BACKGROUND Membrane lipids	131:156	BACKGROUND Membrane lipids play critical roles in the structure and function of membrane-embedded transporters.					
30075778	0	22	theme	transporter	113:123	arg1	MelB					125:128	the Salmonella typhimurium melibiose transporter MelB	76:128	the Salmonella typhimurium melibiose transporter MelB	76:128	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.					
30075778	8	23	theme	circular	1496:1503	arg1	spectroscopy					1515:1526	circular dichroism spectroscopy	1496:1526	circular dichroism spectroscopy	1496:1526	Remarkably, the delipidated MelBSt with only 2-3 bound lipids, regardless of the headgroup species, also exhibits unchanged melting temperature values as shown by circular dichroism spectroscopy.					
30075778	5	24	theme	resonance	758:766	arg1	spectroscopy					776:787	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy	729:787	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments	729:799	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	3	25	from	study	385:389	arg1	effects					398:404	the effects	394:404	the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein	394:511	We present an extensive study on the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein.					
30075778	6	26	theme	PE	1071:1072	arg1	lack					1063:1066	lack	1063:1066	lack of PE or PG	1063:1078	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	8	27	theme	delipidated	1349:1359	arg1	MelBSt					1361:1366	the delipidated MelBSt	1345:1366	the delipidated MelBSt	1345:1366	Remarkably, the delipidated MelBSt with only 2-3 bound lipids, regardless of the headgroup species, also exhibits unchanged melting temperature values as shown by circular dichroism spectroscopy.					
30075778	3	28	theme	phospholipids	418:430	arg1	effects					398:404	the effects	394:404	the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein	394:511	We present an extensive study on the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein.					
30075778	5	29	theme	lipid	827:831	arg1	tails					833:837	lipid tails	827:837	lipid tails	827:837	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	4	30	theme	chromatography	556:569	arg1	experiments					577:587	thin-layer chromatography (TLC) experiments	545:587	thin-layer chromatography (TLC) experiments	545:587	RESULTS Lipidomic analysis and thin-layer chromatography (TLC) experiments reveal that at least one phosphatidylethanolamine (PE) and one phosphatidylglycerol (PG) molecule associate with MelBSt at high affinities.					
30075778	0	31	theme	functional	15:24	arg1	characterization					26:41	Structural and functional characterization	0:41	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.	0:129	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.					
30075778	9	32	theme	glycerol	1561:1568	arg1	backbones					1570:1578	glycerol backbones	1561:1578	glycerol backbones	1561:1578	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	4	33	dep	RESULTS	514:520	arg1	experiments					577:587	thin-layer chromatography (TLC) experiments	545:587	thin-layer chromatography (TLC) experiments	545:587	RESULTS Lipidomic analysis and thin-layer chromatography (TLC) experiments reveal that at least one phosphatidylethanolamine (PE) and one phosphatidylglycerol (PG) molecule associate with MelBSt at high affinities.					
30075778	4	33	dep	RESULTS	514:520	arg1	analysis					532:539	Lipidomic analysis	522:539	Lipidomic analysis	522:539	RESULTS Lipidomic analysis and thin-layer chromatography (TLC) experiments reveal that at least one phosphatidylethanolamine (PE) and one phosphatidylglycerol (PG) molecule associate with MelBSt at high affinities.					
30075778	4	34	theme	Lipidomic	522:530	arg1	analysis					532:539	Lipidomic analysis	522:539	Lipidomic analysis	522:539	RESULTS Lipidomic analysis and thin-layer chromatography (TLC) experiments reveal that at least one phosphatidylethanolamine (PE) and one phosphatidylglycerol (PG) molecule associate with MelBSt at high affinities.					
30075778	10	35	theme	lipid	1767:1771	arg1	headgroups					1773:1782	lipid headgroups	1767:1782	lipid headgroups	1767:1782	(2) The headgroups of PE and PG, but not of CL, play important roles in melibiose transport; however, lipid headgroups do not modulate the folding and stability of MelBSt.					
30075778	0	36	theme	interactions	60:71	arg1	characterization					26:41	Structural and functional characterization	0:41	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.	0:129	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.					
30075778	9	37	theme	PE	1595:1596	arg1	tails					1551:1555	Lipid tails	1545:1555	Lipid tails	1545:1555	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	9	37	theme	PE	1595:1596	arg1	backbones					1570:1578	glycerol backbones	1561:1578	glycerol backbones	1561:1578	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	7	38	theme	MelBSt	1259:1264	arg1	folding					1266:1272	MelBSt folding	1259:1272	MelBSt folding	1259:1272	Interestingly, neither the co-substrate binding (melibiose or Na+) nor MelBSt folding and stability are affected by changing lipid compositions.					
30075778	2	39	theme	Salmonella	243:252	arg1	MelBSt					272:277	MelBSt	272:277	MelBSt	272:277	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	2	39	theme	Salmonella	243:252	arg1	MelB					266:269	Salmonella typhimurium MelB	243:269	Salmonella typhimurium MelB (MelBSt)	243:278	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	2	39	theme	Salmonella	243:252	arg1	translocation					314:326	a symporter coupling melibiose translocation	283:326	a symporter coupling melibiose translocation	283:326	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	3	40	theme	melibiose	467:475	arg1	transport					477:485	the melibiose transport	463:485	the melibiose transport catalyzed by this protein	463:511	We present an extensive study on the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein.					
30075778	9	41	theme	PG	1602:1603	arg1	tails					1551:1555	Lipid tails	1545:1555	Lipid tails	1545:1555	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	9	41	theme	PG	1602:1603	arg1	backbones					1570:1578	glycerol backbones	1561:1578	glycerol backbones	1561:1578	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	6	42	with	Studies	929:935	arg1	strains					1044:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	6	42	with	Studies	929:935	arg1	strains					959:965	lipid-engineered strains	942:965	lipid-engineered strains	942:965	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	2	43	dep	Salmonella	243:252	arg1	typhimurium					254:264	typhimurium	254:264	typhimurium	254:264	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	10	44	theme	MelBSt	1829:1834	arg1	stability					1816:1824	stability	1816:1824	stability	1816:1824	(2) The headgroups of PE and PG, but not of CL, play important roles in melibiose transport; however, lipid headgroups do not modulate the folding and stability of MelBSt.					
30075778	10	44	theme	MelBSt	1829:1834	arg1	folding					1804:1810	folding	1804:1810	folding	1804:1810	(2) The headgroups of PE and PG, but not of CL, play important roles in melibiose transport; however, lipid headgroups do not modulate the folding and stability of MelBSt.					
30075778	6	45	theme	PG	1077:1078	arg1	lack					1063:1066	lack	1063:1066	lack of PE or PG	1063:1078	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	9	46	theme	structure	1644:1652	arg1	of MelBSt					1654:1662	the structure of MelBSt	1640:1662	the structure of MelBSt	1640:1662	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	6	47	theme	melibiose	1139:1147	arg1	transport					1156:1164	both H+- and Na+-coupled melibiose active transport	1114:1164	both H+- and Na+-coupled melibiose active transport to different extents	1114:1185	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	10	48	theme	CL	1709:1710	arg1	headgroups					1673:1682	The headgroups	1669:1682	The headgroups of PE and PG, but not of CL,	1669:1711	(2) The headgroups of PE and PG, but not of CL, play important roles in melibiose transport; however, lipid headgroups do not modulate the folding and stability of MelBSt.					
30075778	1	49	theme	transporters	229:240	arg1	function					199:206	function	199:206	function	199:206	BACKGROUND Membrane lipids play critical roles in the structure and function of membrane-embedded transporters.					
30075778	1	49	theme	transporters	229:240	arg1	structure					185:193	structure	185:193	structure	185:193	BACKGROUND Membrane lipids play critical roles in the structure and function of membrane-embedded transporters.					
30075778	9	50	dep	contribute	1609:1618	arg1	1					1542:1542	1	1542:1542	1	1542:1542	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	10	51	theme	melibiose	1737:1745	arg1	transport					1747:1755	melibiose transport	1737:1755	melibiose transport	1737:1755	(2) The headgroups of PE and PG, but not of CL, play important roles in melibiose transport; however, lipid headgroups do not modulate the folding and stability of MelBSt.					
30075778	5	52	theme	Solid-state	729:739	arg1	spectroscopy					776:787	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy	729:787	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments	729:799	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	1	53	theme	Membrane	142:149	arg1	lipids					151:156	BACKGROUND Membrane lipids	131:156	BACKGROUND Membrane lipids	131:156	BACKGROUND Membrane lipids play critical roles in the structure and function of membrane-embedded transporters.					
30075778	8	54	theme	unchanged	1447:1455	arg1	values					1477:1482	unchanged melting temperature values	1447:1482	unchanged melting temperature values	1447:1482	Remarkably, the delipidated MelBSt with only 2-3 bound lipids, regardless of the headgroup species, also exhibits unchanged melting temperature values as shown by circular dichroism spectroscopy.					
30075778	2	55	dep	cation	335:340	arg1	Li+					348:350	Li+	348:350	Li+	348:350	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	2	55	dep	cation	335:340	arg1	H+					356:357	H+	356:357	H+	356:357	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	2	55	dep	cation	335:340	arg1	Na+					343:345	Na+	343:345	Na+	343:345	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	4	56	dep	phosphatidylethanolamine	614:637	arg1	molecule					678:685	molecule	678:685	molecule	678:685	RESULTS Lipidomic analysis and thin-layer chromatography (TLC) experiments reveal that at least one phosphatidylethanolamine (PE) and one phosphatidylglycerol (PG) molecule associate with MelBSt at high affinities.					
30075778	2	57	theme	coupling	295:302	arg1	MelB					266:269	Salmonella typhimurium MelB	243:269	Salmonella typhimurium MelB (MelBSt)	243:278	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	2	57	theme	coupling	295:302	arg1	translocation					314:326	a symporter coupling melibiose translocation	283:326	a symporter coupling melibiose translocation	283:326	Salmonella typhimurium MelB (MelBSt) is a symporter coupling melibiose translocation with a cation (Na+, Li+, or H+).					
30075778	6	58	theme	CL-deficient	1031:1042	arg1	strains					1044:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains	978:1050	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	8	59	theme	temperature	1465:1475	arg1	values					1477:1482	unchanged melting temperature values	1447:1482	unchanged melting temperature values	1447:1482	Remarkably, the delipidated MelBSt with only 2-3 bound lipids, regardless of the headgroup species, also exhibits unchanged melting temperature values as shown by circular dichroism spectroscopy.					
30075778	5	60	theme	PG	906:907	arg1	headgroups					892:901	headgroups	892:901	headgroups of PG	892:907	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	0	61	theme	Salmonella	80:89	arg1	MelB					125:128	the Salmonella typhimurium melibiose transporter MelB	76:128	the Salmonella typhimurium melibiose transporter MelB	76:128	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.					
30075778	5	62	theme	glycerol	843:850	arg1	backbones					852:860	glycerol backbones	843:860	glycerol backbones	843:860	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	6	63	theme	different	1169:1177	arg1	extents					1179:1185	different extents	1169:1185	different extents	1169:1185	Studies with lipid-engineered strains, including PE-deficient, cardiolipin (CL)- and PG-deficient, or CL-deficient strains, show that lack of PE or PG, however not CL, largely inhibits both H+- and Na+-coupled melibiose active transport to different extents.					
30075778	5	64	theme	nuclear	741:747	arg1	ssNMR					769:773	ssNMR	769:773	ssNMR	769:773	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	5	64	theme	nuclear	741:747	arg1	resonance					758:766	nuclear magnetic resonance	741:766	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments	729:799	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	0	65	theme	melibiose	103:111	arg1	MelB					125:128	the Salmonella typhimurium melibiose transporter MelB	76:128	the Salmonella typhimurium melibiose transporter MelB	76:128	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.					
30075778	3	66	from	effects	398:404	arg1	structure					439:447	the structure	435:447	the structure of MelBSt and the melibiose transport catalyzed by this protein	435:511	We present an extensive study on the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein.					
30075778	8	67	with	MelBSt	1361:1366	arg1	lipids					1388:1393	only 2-3 bound lipids	1373:1393	only 2-3 bound lipids	1373:1393	Remarkably, the delipidated MelBSt with only 2-3 bound lipids, regardless of the headgroup species, also exhibits unchanged melting temperature values as shown by circular dichroism spectroscopy.					
30075778	0	68	theme	MelB	125:128	arg1	interactions					60:71	protein-lipid interactions	46:71	protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB	46:128	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.					
30075778	8	69	theme	dichroism	1505:1513	arg1	spectroscopy					1515:1526	circular dichroism spectroscopy	1496:1526	circular dichroism spectroscopy	1496:1526	Remarkably, the delipidated MelBSt with only 2-3 bound lipids, regardless of the headgroup species, also exhibits unchanged melting temperature values as shown by circular dichroism spectroscopy.					
30075778	0	70	theme	Structural	0:9	arg1	characterization					26:41	Structural and functional characterization	0:41	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.	0:129	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.					
30075778	5	71	theme	magnetic	749:756	arg1	ssNMR					769:773	ssNMR	769:773	ssNMR	769:773	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	5	71	theme	magnetic	749:756	arg1	resonance					758:766	nuclear magnetic resonance	741:766	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments	729:799	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	7	72	dep	binding	1228:1234	arg1	melibiose					1237:1245	melibiose	1237:1245	melibiose	1237:1245	Interestingly, neither the co-substrate binding (melibiose or Na+) nor MelBSt folding and stability are affected by changing lipid compositions.					
30075778	7	72	dep	binding	1228:1234	arg1	Na+					1250:1252	Na+	1250:1252	Na+	1250:1252	Interestingly, neither the co-substrate binding (melibiose or Na+) nor MelBSt folding and stability are affected by changing lipid compositions.					
30075778	1	73	dep	structure	185:193	arg1	the					181:183	the	181:183	the	181:183	BACKGROUND Membrane lipids play critical roles in the structure and function of membrane-embedded transporters.					
30075778	5	74	theme	tails	833:837	arg1	presence					815:822	the presence	811:822	the presence of lipid tails and glycerol backbones that co-purified with MelBSt	811:889	Solid-state nuclear magnetic resonance (ssNMR) spectroscopy experiments confirmed the presence of lipid tails and glycerol backbones that co-purified with MelBSt; headgroups of PG were also observed.					
30075778	0	75	dep	Salmonella	80:89	arg1	typhimurium					91:101	typhimurium	91:101	typhimurium	91:101	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.					
30075778	3	76	theme	extensive	375:383	arg1	study					385:389	an extensive study	372:389	an extensive study on the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein	372:511	We present an extensive study on the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein.					
30075778	0	77	theme	protein-lipid	46:58	arg1	interactions					60:71	protein-lipid interactions	46:71	protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB	46:128	Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB.					
30075778	3	78	theme	specific	409:416	arg1	phospholipids					418:430	specific phospholipids	409:430	specific phospholipids	409:430	We present an extensive study on the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein.					
30075778	3	79	theme	MelBSt	452:457	arg1	structure					439:447	the structure	435:447	the structure of MelBSt and the melibiose transport catalyzed by this protein	435:511	We present an extensive study on the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein.					
30075778	9	80	theme	interacting	1583:1593	arg1	PE					1595:1596	interacting PE	1583:1596	interacting PE	1583:1596	CONCLUSIONS (1) Lipid tails and glycerol backbones of interacting PE and PG may contribute to the stability of the structure of MelBSt.					
30075778	4	81	theme	thin-layer	545:554	arg1	TLC					572:574	TLC	572:574	TLC	572:574	RESULTS Lipidomic analysis and thin-layer chromatography (TLC) experiments reveal that at least one phosphatidylethanolamine (PE) and one phosphatidylglycerol (PG) molecule associate with MelBSt at high affinities.					
30075778	4	81	theme	thin-layer	545:554	arg1	chromatography					556:569	thin-layer chromatography	545:569	thin-layer chromatography (TLC) experiments	545:587	RESULTS Lipidomic analysis and thin-layer chromatography (TLC) experiments reveal that at least one phosphatidylethanolamine (PE) and one phosphatidylglycerol (PG) molecule associate with MelBSt at high affinities.					
30075778	8	82	theme	bound	1382:1386	arg1	lipids					1388:1393	only 2-3 bound lipids	1373:1393	only 2-3 bound lipids	1373:1393	Remarkably, the delipidated MelBSt with only 2-3 bound lipids, regardless of the headgroup species, also exhibits unchanged melting temperature values as shown by circular dichroism spectroscopy.					
30075778	3	83	theme	transport	477:485	arg1	structure					439:447	the structure	435:447	the structure of MelBSt and the melibiose transport catalyzed by this protein	435:511	We present an extensive study on the effects of specific phospholipids on the structure of MelBSt and the melibiose transport catalyzed by this protein.					
30075778	7	84	theme	lipid	1313:1317	arg1	compositions					1319:1330	lipid compositions	1313:1330	lipid compositions	1313:1330	Interestingly, neither the co-substrate binding (melibiose or Na+) nor MelBSt folding and stability are affected by changing lipid compositions.					
30075778	10	85	dep	folding	1804:1810	arg1	the					1800:1802	the	1800:1802	the	1800:1802	(2) The headgroups of PE and PG, but not of CL, play important roles in melibiose transport; however, lipid headgroups do not modulate the folding and stability of MelBSt.					
30075778	10	86	theme	PE	1687:1688	arg1	headgroups					1673:1682	The headgroups	1669:1682	The headgroups of PE and PG, but not of CL,	1669:1711	(2) The headgroups of PE and PG, but not of CL, play important roles in melibiose transport; however, lipid headgroups do not modulate the folding and stability of MelBSt.					
29752096	3	0	theme	salt	695:698	arg1	solutions					700:708	salt solutions	695:708	salt solutions	695:708	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	1	1	theme	pharmaceutical	163:176	arg1	industry					178:185	pharmaceutical industry	163:185	pharmaceutical industry	163:185	The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification.					
29752096	3	2	theme	gel	678:680	arg1	stability					682:690	an improved gel stability	666:690	an improved gel stability in salt solutions	666:708	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	3	theme	as-prepared	616:626	arg1	Ca-SA/GO					628:635	the as-prepared Ca-SA/GO	612:635	the as-prepared Ca-SA/GO	612:635	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	2	4	theme	sodium	378:383	arg1	alginate					385:392	environmentally benign sodium alginate	355:392	environmentally benign sodium alginate (SA)	355:397	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	4	theme	sodium	378:383	arg1	SA					395:396	SA	395:396	SA	395:396	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	5	5	theme	lysozyme	1016:1023	arg1	capacity					1036:1043	the lysozyme adsorption capacity	1012:1043	the lysozyme adsorption capacity of Ca-SA/GO	1012:1055	Batch adsorption experiments found that the lysozyme adsorption capacity of Ca-SA/GO can reach 278.28 mg g-1 and it can be regenerated and reused at least 4 times.					
29752096	4	6	theme	SA	921:922	arg1	matrix					924:929	SA matrix	921:929	SA matrix	921:929	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	4	7	theme	hydrogen	852:859	arg1	bonding					861:867	hydrogen bonding	852:867	hydrogen bonding	852:867	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	2	8	dep	oxide	322:326	arg1	GO					329:330	GO	329:330	GO	329:330	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	0	9	theme	mechanical	94:103	arg1	property					105:112	mechanical property	94:112	mechanical property	94:112	Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property.					
29752096	5	10	theme	Ca-SA/GO	1048:1055	arg1	capacity					1036:1043	the lysozyme adsorption capacity	1012:1043	the lysozyme adsorption capacity of Ca-SA/GO	1012:1055	Batch adsorption experiments found that the lysozyme adsorption capacity of Ca-SA/GO can reach 278.28 mg g-1 and it can be regenerated and reused at least 4 times.					
29752096	3	11	theme	pure	562:565	arg1	Ca-SA					604:608	Ca-SA	604:608	Ca-SA	604:608	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	11	theme	pure	562:565	arg1	beads					597:601	pure Ca2+ crosslinked alginate gel beads	562:601	pure Ca2+ crosslinked alginate gel beads (Ca-SA)	562:609	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	2	12	theme	graphene	313:320	arg1	oxide					322:326	graphene oxide	313:326	graphene oxide (GO)	313:331	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	13	theme	benign	371:376	arg1	alginate					385:392	environmentally benign sodium alginate	355:392	environmentally benign sodium alginate (SA)	355:397	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	13	theme	benign	371:376	arg1	SA					395:396	SA	395:396	SA	395:396	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	4	14	theme	Ca-SA	818:822	arg1	matrix					824:829	the Ca-SA matrix	814:829	the Ca-SA matrix	814:829	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	2	15	theme	alginate/graphene	429:445	arg1	beads					467:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads	410:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions	410:545	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	15	theme	alginate/graphene	429:445	arg1	Ca-SA/GO					474:481	Ca-SA/GO	474:481	Ca-SA/GO	474:481	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	7	16	theme	regeneration	1273:1284	arg1	ability					1286:1292	regeneration ability	1273:1292	regeneration ability	1273:1292	With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.					
29752096	7	17	with	adsorbent	1329:1337	arg1	stability					1237:1245	good stability	1232:1245	good stability	1232:1245	With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.					
29752096	7	17	with	adsorbent	1329:1337	arg1	capacity					1259:1266	adsorption capacity	1248:1266	adsorption capacity	1248:1266	With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.					
29752096	7	17	with	adsorbent	1329:1337	arg1	ability					1286:1292	regeneration ability	1273:1292	regeneration ability	1273:1292	With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.					
29752096	6	18	theme	dynamic	1198:1204	arg1	property					1217:1224	excellent dynamic adsorption property	1188:1224	excellent dynamic adsorption property	1188:1224	Moreover, in column adsorption, the Ca-SA/GO showed excellent dynamic adsorption property.					
29752096	1	19	theme	food	191:194	arg1	industry					196:203	food industry	191:203	food industry	191:203	The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification.					
29752096	3	20	from	performance	735:745	arg1	solutions					700:708	salt solutions	695:708	salt solutions	695:708	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	21	from	degree	658:663	arg1	solutions					700:708	salt solutions	695:708	salt solutions	695:708	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	22	theme	alginate	584:591	arg1	Ca-SA					604:608	Ca-SA	604:608	Ca-SA	604:608	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	22	theme	alginate	584:591	arg1	beads					597:601	pure Ca2+ crosslinked alginate gel beads	562:601	pure Ca2+ crosslinked alginate gel beads (Ca-SA)	562:609	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	4	23	theme	bonding	861:867	arg1	existence					839:847	the existence	835:847	the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix	835:929	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	4	24	from	distribution	785:796	arg1	matrix					824:829	the Ca-SA matrix	814:829	the Ca-SA matrix	814:829	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	6	25	theme	adsorption	1206:1215	arg1	property					1217:1224	excellent dynamic adsorption property	1188:1224	excellent dynamic adsorption property	1188:1224	Moreover, in column adsorption, the Ca-SA/GO showed excellent dynamic adsorption property.					
29752096	1	26	dep	industry	178:185	arg1	the					159:161	the	159:161	the	159:161	The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification.					
29752096	5	27	theme	adsorption	1025:1034	arg1	capacity					1036:1043	the lysozyme adsorption capacity	1012:1043	the lysozyme adsorption capacity of Ca-SA/GO	1012:1055	Batch adsorption experiments found that the lysozyme adsorption capacity of Ca-SA/GO can reach 278.28 mg g-1 and it can be regenerated and reused at least 4 times.					
29752096	2	28	theme	gel	463:465	arg1	beads					467:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads	410:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions	410:545	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	28	theme	gel	463:465	arg1	Ca-SA/GO					474:481	Ca-SA/GO	474:481	Ca-SA/GO	474:481	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	7	29	theme	aqueous	1357:1363	arg1	solutions					1365:1373	aqueous solutions	1357:1373	aqueous solutions	1357:1373	With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.					
29752096	1	30	from	applications	131:142	arg1	industry					196:203	food industry	191:203	food industry	191:203	The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification.					
29752096	1	30	from	applications	131:142	arg1	industry					178:185	pharmaceutical industry	163:185	pharmaceutical industry	163:185	The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification.					
29752096	3	31	theme	Ca2+	567:570	arg1	Ca-SA					604:608	Ca-SA	604:608	Ca-SA	604:608	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	31	theme	Ca2+	567:570	arg1	beads					597:601	pure Ca2+ crosslinked alginate gel beads	562:601	pure Ca2+ crosslinked alginate gel beads (Ca-SA)	562:609	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	2	32	theme	oxide	447:451	arg1	beads					467:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads	410:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions	410:545	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	32	theme	oxide	447:451	arg1	Ca-SA/GO					474:481	Ca-SA/GO	474:481	Ca-SA/GO	474:481	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	0	33	theme	lysozyme	14:21	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property.	0:113	Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property.					
29752096	3	34	theme	higher	717:722	arg1	performance					735:745	a higher mechanical performance	715:745	a higher mechanical performance	715:745	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	4	35	theme	adhesion	890:897	arg1	existence					839:847	the existence	835:847	the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix	835:929	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	3	36	theme	lower	643:647	arg1	degree					658:663	a lower swelling degree	641:663	a lower swelling degree	641:663	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	37	theme	crosslinked	572:582	arg1	Ca-SA					604:608	Ca-SA	604:608	Ca-SA	604:608	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	37	theme	crosslinked	572:582	arg1	beads					597:601	pure Ca2+ crosslinked alginate gel beads	562:601	pure Ca2+ crosslinked alginate gel beads (Ca-SA)	562:609	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	1	38	theme	efficient	218:226	arg1	techniques					247:256	more efficient and cost-effective techniques	213:256	more efficient and cost-effective techniques for its separation/purification	213:288	The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification.					
29752096	0	39	theme	oxide	44:48	arg1	beads					60:64	alginate/graphene oxide composite beads	26:64	alginate/graphene oxide composite beads with enhanced stability and mechanical property	26:112	Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property.					
29752096	3	40	theme	mechanical	724:733	arg1	performance					735:745	a higher mechanical performance	715:745	a higher mechanical performance	715:745	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	41	theme	swelling	649:656	arg1	degree					658:663	a lower swelling degree	641:663	a lower swelling degree	641:663	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	2	42	dep	crosslinked	417:427	arg1	Ca2+					412:415	Ca2+	412:415	Ca2+	412:415	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	43	theme	crosslinked	417:427	arg1	beads					467:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads	410:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions	410:545	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	43	theme	crosslinked	417:427	arg1	Ca-SA/GO					474:481	Ca-SA/GO	474:481	Ca-SA/GO	474:481	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	0	44	theme	alginate/graphene	26:42	arg1	beads					60:64	alginate/graphene oxide composite beads	26:64	alginate/graphene oxide composite beads with enhanced stability and mechanical property	26:112	Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property.					
29752096	3	45	from	stability	682:690	arg1	solutions					700:708	salt solutions	695:708	salt solutions	695:708	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	1	46	theme	cost-effective	232:245	arg1	techniques					247:256	more efficient and cost-effective techniques	213:256	more efficient and cost-effective techniques for its separation/purification	213:288	The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification.					
29752096	4	47	dep	filler	910:915	arg1	GO					907:908	GO	907:908	GO	907:908	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	4	48	theme	sheets	804:809	arg1	distribution					785:796	the uniform distribution	773:796	the uniform distribution of GO sheets in the Ca-SA matrix	773:829	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	5	49	theme	Batch	972:976	arg1	experiments					989:999	Batch adsorption experiments	972:999	Batch adsorption experiments	972:999	Batch adsorption experiments found that the lysozyme adsorption capacity of Ca-SA/GO can reach 278.28 mg g-1 and it can be regenerated and reused at least 4 times.					
29752096	1	50	theme	large-scale	119:129	arg1	applications					131:142	The large-scale applications	115:142	The large-scale applications of lysozyme in the pharmaceutical industry and food industry	115:203	The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification.					
29752096	0	51	theme	composite	50:58	arg1	beads					60:64	alginate/graphene oxide composite beads	26:64	alginate/graphene oxide composite beads with enhanced stability and mechanical property	26:112	Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property.					
29752096	3	52	contain	has	637:639	arg2	performance					735:745	a higher mechanical performance	715:745	a higher mechanical performance	715:745	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	52	contain	has	637:639	arg2	degree					658:663	a lower swelling degree	641:663	a lower swelling degree	641:663	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	52	contain	has	637:639	arg2	stability					682:690	an improved gel stability	666:690	an improved gel stability in salt solutions	666:708	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	52	contain	has	637:639	arg1	Ca-SA/GO					628:635	the as-prepared Ca-SA/GO	612:635	the as-prepared Ca-SA/GO	612:635	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	2	53	link	crosslinked	417:427	arg1	beads					467:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads	410:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions	410:545	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	53	link	crosslinked	417:427	arg1	Ca-SA/GO					474:481	Ca-SA/GO	474:481	Ca-SA/GO	474:481	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	3	54	theme	improved	669:676	arg1	stability					682:690	an improved gel stability	666:690	an improved gel stability in salt solutions	666:708	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	7	55	theme	good	1232:1235	arg1	stability					1237:1245	good stability	1232:1245	good stability	1232:1245	With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.					
29752096	4	56	theme	uniform	777:783	arg1	distribution					785:796	the uniform distribution	773:796	the uniform distribution of GO sheets in the Ca-SA matrix	773:829	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	4	57	theme	interfacial	878:888	arg1	adhesion					890:897	high interfacial adhesion	873:897	high interfacial adhesion	873:897	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	2	58	used	used	500:503	arg2	Ca-SA/GO					474:481	Ca-SA/GO	474:481	Ca-SA/GO	474:481	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	58	used	used	500:503	arg2	beads					467:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads	410:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions	410:545	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	59	theme	present	298:304	arg1	study					306:310	the present study	294:310	the present study	294:310	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	6	60	theme	excellent	1188:1196	arg1	property					1217:1224	excellent dynamic adsorption property	1188:1224	excellent dynamic adsorption property	1188:1224	Moreover, in column adsorption, the Ca-SA/GO showed excellent dynamic adsorption property.					
29752096	4	61	theme	high	873:876	arg1	adhesion					890:897	high interfacial adhesion	873:897	high interfacial adhesion	873:897	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	6	62	theme	column	1149:1154	arg1	adsorption					1156:1165	column adsorption	1149:1165	column adsorption	1149:1165	Moreover, in column adsorption, the Ca-SA/GO showed excellent dynamic adsorption property.					
29752096	0	63	with	beads	60:64	arg1	stability					80:88	enhanced stability	71:88	enhanced stability	71:88	Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property.					
29752096	0	63	with	beads	60:64	arg1	property					105:112	mechanical property	94:112	mechanical property	94:112	Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property.					
29752096	1	64	theme	lysozyme	147:154	arg1	applications					131:142	The large-scale applications	115:142	The large-scale applications of lysozyme in the pharmaceutical industry and food industry	115:203	The large-scale applications of lysozyme in the pharmaceutical industry and food industry require more efficient and cost-effective techniques for its separation/purification.					
29752096	5	65	theme	adsorption	978:987	arg1	experiments					989:999	Batch adsorption experiments	972:999	Batch adsorption experiments	972:999	Batch adsorption experiments found that the lysozyme adsorption capacity of Ca-SA/GO can reach 278.28 mg g-1 and it can be regenerated and reused at least 4 times.					
29752096	0	66	theme	enhanced	71:78	arg1	stability					80:88	enhanced stability	71:88	enhanced stability	71:88	Adsorption of lysozyme by alginate/graphene oxide composite beads with enhanced stability and mechanical property.					
29752096	7	67	theme	adsorption	1248:1257	arg1	capacity					1259:1266	adsorption capacity	1248:1266	adsorption capacity	1248:1266	With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.					
29752096	4	68	dep	sheets	804:809	arg1	GO					801:802	GO	801:802	GO	801:802	This can be explained by the uniform distribution of GO sheets in the Ca-SA matrix and the existence of hydrogen bonding and high interfacial adhesion between GO filler and SA matrix demonstrated by SEM, FTIR, XRD, and TGA.					
29752096	3	69	link	crosslinked	572:582	arg1	Ca-SA					604:608	Ca-SA	604:608	Ca-SA	604:608	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	69	link	crosslinked	572:582	arg1	beads					597:601	pure Ca2+ crosslinked alginate gel beads	562:601	pure Ca2+ crosslinked alginate gel beads (Ca-SA)	562:609	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	2	70	theme	composite	453:461	arg1	beads					467:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads	410:471	a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions	410:545	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	2	70	theme	composite	453:461	arg1	Ca-SA/GO					474:481	Ca-SA/GO	474:481	Ca-SA/GO	474:481	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29752096	3	71	theme	gel	593:595	arg1	Ca-SA					604:608	Ca-SA	604:608	Ca-SA	604:608	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	3	71	theme	gel	593:595	arg1	beads					597:601	pure Ca2+ crosslinked alginate gel beads	562:601	pure Ca2+ crosslinked alginate gel beads (Ca-SA)	562:609	Compared with pure Ca2+ crosslinked alginate gel beads (Ca-SA), the as-prepared Ca-SA/GO has a lower swelling degree, an improved gel stability in salt solutions, and a higher mechanical performance.					
29752096	7	72	from	solutions	1365:1373	arg1	Ca-SA/GO					1299:1306	the Ca-SA/GO	1295:1306	the Ca-SA/GO	1295:1306	With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.					
29752096	7	72	from	solutions	1365:1373	arg1	adsorbent					1329:1337	adsorbent	1329:1337	adsorbent	1329:1337	With good stability, adsorption capacity, and regeneration ability, the Ca-SA/GO could be a promising adsorbent for lysozyme from aqueous solutions.					
29752096	2	73	theme	aqueous	529:535	arg1	solutions					537:545	aqueous solutions	529:545	aqueous solutions	529:545	In the present study, graphene oxide (GO) was encapsulated into environmentally benign sodium alginate (SA) to prepare a Ca2+ crosslinked alginate/graphene oxide composite gel beads (Ca-SA/GO) which were then used to adsorb lysozyme from aqueous solutions.					
29939754	9	0	from	complexity	1802:1811	arg1	constructs					1830:1839	3D bioprinted constructs	1816:1839	3D bioprinted constructs	1816:1839	Such tailorable bioink platforms will enable increased complexity in 3D bioprinted constructs.					
29939754	7	1	dep	biofunctionality	1523:1538	arg1	shown					1544:1548	shown	1544:1548	shown via RGD attachment	1544:1567	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	1	2	theme	biomaterials	155:166	arg1	deposition					141:150	precise and controlled 3D deposition	115:150	precise and controlled 3D deposition of biomaterials	115:166	Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner.					
29939754	8	3	theme	bioink	1730:1735	arg1	platform					1737:1744	a chemically similar bioink platform	1709:1744	a chemically similar bioink platform	1709:1744	This modularity enables the creation of multizonal and multicellular constructs utilizing a chemically similar bioink platform.					
29939754	7	4	theme	expected	1578:1585	arg1	application					1606:1616	the expected tissue-engineering application	1574:1616	the expected tissue-engineering application	1574:1616	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	9	5	theme	bioink	1763:1768	arg1	platforms					1770:1778	Such tailorable bioink platforms	1747:1778	Such tailorable bioink platforms	1747:1778	Such tailorable bioink platforms will enable increased complexity in 3D bioprinted constructs.					
29939754	2	6	theme	biomaterial	353:363	arg1	compositions					365:376	biomaterial compositions	353:376	biomaterial compositions	353:376	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	5	7	theme	cross-linkers	1022:1034	arg1	length					985:990	length	985:990	length	985:990	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	5	7	theme	cross-linkers	1022:1034	arg1	structure					997:1005	structure	997:1005	structure	997:1005	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	5	7	theme	cross-linkers	1022:1034	arg1	concentration					970:982	concentration	970:982	concentration	970:982	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	6	8	theme	low	1144:1146	arg1	concentrations					1148:1161	The low concentrations	1140:1161	The low concentrations	1140:1161	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	2	9	theme	Cell-laden	225:234	arg1	hydrogel					236:243	Cell-laden hydrogel	225:243	Cell-laden hydrogel ("bioink") bioprinting	225:266	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	2	9	theme	Cell-laden	225:234	arg1	"					253:253	"bioink"	246:253	"bioink"	246:253	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	4	10	theme	rapid	790:794	arg1	mechanism					831:839	a rapid UV-induced thiol-ene cross-linking mechanism	788:839	a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation	788:884	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	6	11	theme	wt	1186:1187	arg1	%					1189:1189	<2 wt %	1183:1189	<2 wt %	1183:1189	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	6	11	theme	wt	1186:1187	arg1	alginate					1166:1173	alginate	1166:1173	alginate needed (<2 wt %)	1166:1190	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	8	12	theme	constructs	1688:1697	arg1	creation					1647:1654	the creation	1643:1654	the creation of multizonal and multicellular constructs utilizing a chemically similar bioink platform	1643:1744	This modularity enables the creation of multizonal and multicellular constructs utilizing a chemically similar bioink platform.					
29939754	7	13	theme	properties	1493:1502	arg1	biofunctionality					1523:1538	the gel's biofunctionality	1513:1538	not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment)	1451:1568	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	7	13	theme	properties	1493:1502	arg1	design					1473:1478	the rational design	1460:1478	not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment)	1451:1568	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	8	14	theme	multicellular	1674:1686	arg1	constructs					1688:1697	multizonal and multicellular constructs	1659:1697	multizonal and multicellular constructs utilizing a chemically similar bioink platform	1659:1744	This modularity enables the creation of multizonal and multicellular constructs utilizing a chemically similar bioink platform.					
29939754	5	15	theme	dithiol	1010:1016	arg1	cross-linkers					1022:1034	dithiol PEG cross-linkers	1010:1034	dithiol PEG cross-linkers	1010:1034	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	5	16	theme	swelling	906:913	arg1	tunable					947:953	tunable	947:953	tunable	947:953	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	5	16	theme	swelling	906:913	arg1	properties					915:924	The mechanical and swelling properties	887:924	The mechanical and swelling properties of the hydrogels	887:941	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	4	17	theme	system	720:725	arg1	bioink					743:748	a cell-laden bioink	730:748	a cell-laden bioink for extrusion-based bioprinting	730:780	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	4	17	theme	system	720:725	arg1	development					668:678	the development	664:678	the development of a norbornene functionalized alginate system	664:725	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	4	18	theme	cell-laden	732:741	arg1	bioink					743:748	a cell-laden bioink	730:748	a cell-laden bioink for extrusion-based bioprinting	730:780	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	4	18	theme	cell-laden	732:741	arg1	development					668:678	the development	664:678	the development of a norbornene functionalized alginate system	664:725	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	6	19	theme	viability	1276:1284	arg1	ability					1294:1300	the ability	1290:1300	the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions	1290:1394	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	6	19	theme	viability	1276:1284	arg1	maintenance					1251:1261	the maintenance	1247:1261	the maintenance of high cell viability	1247:1284	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	4	20	theme	thiol-ene	807:815	arg1	mechanism					831:839	a rapid UV-induced thiol-ene cross-linking mechanism	788:839	a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation	788:884	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	3	21	theme	few	477:479	arg1	systems					490:496	few hydrogel systems	477:496	few hydrogel systems	477:496	Despite this promise, few hydrogel systems are easily available and suitable as bioinks, with even fewer systems allowing for molecular design of mechanical and biological properties.					
29939754	6	22	theme	bioprinting	1373:1383	arg1	conditions					1385:1394	identical bioprinting conditions	1363:1394	identical bioprinting conditions	1363:1394	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	6	23	theme	cell	1271:1274	arg1	viability					1276:1284	high cell viability	1266:1284	high cell viability	1266:1284	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	3	24	theme	fewer	554:558	arg1	systems					560:566	even fewer systems	549:566	even fewer systems	549:566	Despite this promise, few hydrogel systems are easily available and suitable as bioinks, with even fewer systems allowing for molecular design of mechanical and biological properties.					
29939754	5	25	with	cross-linking	1090:1102	arg1	ions					1118:1121	divalent ions	1109:1121	divalent ions such as calcium	1109:1137	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	5	25	with	cross-linking	1090:1102	arg1	calcium					1131:1137	calcium	1131:1137	calcium	1131:1137	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	1	26	theme	predesigned	173:183	arg1	manner					217:222	a predesigned, customizable, and reproducible manner	171:222	a predesigned, customizable, and reproducible manner	171:222	Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner.					
29939754	7	27	theme	materials	1483:1491	arg1	properties					1493:1502	materials properties	1483:1502	materials properties	1483:1502	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	6	28	theme	high	1266:1269	arg1	viability					1276:1284	high cell viability	1266:1284	high cell viability	1266:1284	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	1	29	theme	powerful	84:91	arg1	technique					93:101	a powerful technique	82:101	a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner	82:222	Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner.					
29939754	1	29	theme	powerful	84:91	arg1	Bioprinting					67:77	Bioprinting	67:77	Bioprinting	67:77	Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner.					
29939754	4	30	theme	norbornene	685:694	arg1	system					720:725	a norbornene functionalized alginate system	683:725	a norbornene functionalized alginate system	683:725	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	0	31	theme	Thiol-Ene	0:8	arg1	Alginate					10:17	Thiol-Ene Alginate	0:17	Thiol-Ene Alginate	0:17	Thiol-Ene Alginate Hydrogels as Versatile Bioinks for Bioprinting.					
29939754	7	32	theme	rational	1464:1471	arg1	design					1473:1478	the rational design	1460:1478	not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment)	1451:1568	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	9	33	theme	Such	1747:1750	arg1	platforms					1770:1778	Such tailorable bioink platforms	1747:1778	Such tailorable bioink platforms	1747:1778	Such tailorable bioink platforms will enable increased complexity in 3D bioprinted constructs.					
29939754	2	34	theme	engineering	306:316	arg1	applications					318:329	tissue engineering applications	299:329	tissue engineering applications	299:329	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	1	35	theme	customizable	186:197	arg1	manner					217:222	a predesigned, customizable, and reproducible manner	171:222	a predesigned, customizable, and reproducible manner	171:222	Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner.					
29939754	5	36	theme	mechanical	891:900	arg1	tunable					947:953	tunable	947:953	tunable	947:953	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	5	36	theme	mechanical	891:900	arg1	properties					915:924	The mechanical and swelling properties	887:924	The mechanical and swelling properties of the hydrogels	887:941	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	6	37	theme	identical	1363:1371	arg1	conditions					1385:1394	identical bioprinting conditions	1363:1394	identical bioprinting conditions	1363:1394	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	5	38	theme	secondary	1080:1088	arg1	cross-linking					1090:1102	secondary cross-linking	1080:1102	secondary cross-linking with divalent ions such as calcium	1080:1137	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	2	39	theme	tissue	299:304	arg1	applications					318:329	tissue engineering applications	299:329	tissue engineering applications	299:329	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	4	40	theme	kinetic	862:868	arg1	formation					876:884	acrylate kinetic chain formation	853:884	acrylate kinetic chain formation	853:884	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	4	41	theme	extrusion-based	754:768	arg1	bioprinting					770:780	extrusion-based bioprinting	754:780	extrusion-based bioprinting	754:780	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	7	42	theme	tissue-engineering	1587:1604	arg1	application					1606:1616	the expected tissue-engineering application	1574:1616	the expected tissue-engineering application	1574:1616	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	5	43	theme	PEG	1018:1020	arg1	cross-linkers					1022:1034	dithiol PEG cross-linkers	1010:1034	dithiol PEG cross-linkers	1010:1034	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	6	44	theme	rapid	1212:1216	arg1	gelation					1226:1233	their rapid in situ gelation	1206:1233	their rapid in situ gelation	1206:1233	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	3	45	theme	molecular	581:589	arg1	design					591:596	molecular design	581:596	molecular design of mechanical and biological properties	581:636	Despite this promise, few hydrogel systems are easily available and suitable as bioinks, with even fewer systems allowing for molecular design of mechanical and biological properties.					
29939754	5	46	theme	hydrogels	933:941	arg1	tunable					947:953	tunable	947:953	tunable	947:953	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	5	46	theme	hydrogels	933:941	arg1	properties					915:924	The mechanical and swelling properties	887:924	The mechanical and swelling properties of the hydrogels	887:941	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	8	47	theme	multizonal	1659:1668	arg1	constructs					1688:1697	multizonal and multicellular constructs	1659:1697	multizonal and multicellular constructs utilizing a chemically similar bioink platform	1659:1744	This modularity enables the creation of multizonal and multicellular constructs utilizing a chemically similar bioink platform.					
29939754	1	48	theme	reproducible	204:215	arg1	manner					217:222	a predesigned, customizable, and reproducible manner	171:222	a predesigned, customizable, and reproducible manner	171:222	Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner.					
29939754	3	49	theme	biological	616:625	arg1	properties					627:636	mechanical and biological properties	601:636	mechanical and biological properties	601:636	Despite this promise, few hydrogel systems are easily available and suitable as bioinks, with even fewer systems allowing for molecular design of mechanical and biological properties.					
29939754	7	50	theme	platform	1427:1434	arg1	design					1436:1441	this bioink platform design	1415:1441	this bioink platform design	1415:1441	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	2	51	theme	well-defined	427:438	arg1	architectures					440:452	spatially well-defined architectures	417:452	spatially well-defined architectures	417:452	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	0	52	theme	Versatile	32:40	arg1	Bioinks					42:48	Versatile Bioinks	32:48	Versatile Bioinks for Bioprinting	32:64	Thiol-Ene Alginate Hydrogels as Versatile Bioinks for Bioprinting.					
29939754	9	53	theme	tailorable	1752:1761	arg1	platforms					1770:1778	Such tailorable bioink platforms	1747:1778	Such tailorable bioink platforms	1747:1778	Such tailorable bioink platforms will enable increased complexity in 3D bioprinted constructs.					
29939754	6	54	theme	multibioink	1335:1345	arg1	constructs					1347:1356	large multilayer or multibioink constructs	1315:1356	constructs	1347:1356	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	7	55	dep	shown	1544:1548	arg1	as					1541:1542	as	1541:1542	as	1541:1542	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	3	56	theme	properties	627:636	arg1	design					591:596	molecular design	581:596	molecular design of mechanical and biological properties	581:636	Despite this promise, few hydrogel systems are easily available and suitable as bioinks, with even fewer systems allowing for molecular design of mechanical and biological properties.					
29939754	3	57	theme	hydrogel	481:488	arg1	systems					490:496	few hydrogel systems	477:496	few hydrogel systems	477:496	Despite this promise, few hydrogel systems are easily available and suitable as bioinks, with even fewer systems allowing for molecular design of mechanical and biological properties.					
29939754	5	58	dep	concentration	970:982	arg1	the					966:968	the	966:968	the	966:968	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	7	59	theme	RGD	1554:1556	arg1	attachment					1558:1567	RGD attachment	1554:1567	RGD attachment	1554:1567	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	3	60	theme	mechanical	601:610	arg1	properties					627:636	mechanical and biological properties	601:636	mechanical and biological properties	601:636	Despite this promise, few hydrogel systems are easily available and suitable as bioinks, with even fewer systems allowing for molecular design of mechanical and biological properties.					
29939754	1	61	theme	precise	115:121	arg1	deposition					141:150	precise and controlled 3D deposition	115:150	precise and controlled 3D deposition of biomaterials	115:166	Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner.					
29939754	9	62	theme	increased	1792:1800	arg1	complexity					1802:1811	increased complexity	1792:1811	increased complexity in 3D bioprinted constructs	1792:1839	Such tailorable bioink platforms will enable increased complexity in 3D bioprinted constructs.					
29939754	4	63	theme	chain	870:874	arg1	formation					876:884	acrylate kinetic chain formation	853:884	acrylate kinetic chain formation	853:884	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	6	64	dep	in	1218:1219	arg1	situ					1221:1224	situ	1221:1224	situ	1221:1224	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	4	65	theme	acrylate	853:860	arg1	formation					876:884	acrylate kinetic chain formation	853:884	acrylate kinetic chain formation	853:884	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	6	66	theme	large	1315:1319	arg1	multilayer					1321:1330	large multilayer or multibioink constructs	1315:1356	multilayer	1321:1330	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	6	67	dep	needed	1175:1180	arg1	%					1189:1189	<2 wt %	1183:1189	<2 wt %	1183:1189	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	6	67	dep	needed	1175:1180	arg1	alginate					1166:1173	alginate	1166:1173	alginate needed (<2 wt %)	1166:1190	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	2	68	theme	hydrogel	236:243	arg1	bioprinting					256:266	Cell-laden hydrogel ("bioink") bioprinting	225:266	Cell-laden hydrogel ("bioink") bioprinting	225:266	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	1	69	theme	controlled	127:136	arg1	deposition					141:150	precise and controlled 3D deposition	115:150	precise and controlled 3D deposition of biomaterials	115:166	Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner.					
29939754	6	70	theme	alginate	1166:1173	arg1	concentrations					1148:1161	The low concentrations	1140:1161	The low concentrations	1140:1161	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	7	71	theme	design	1436:1441	arg1	modularity					1401:1410	The modularity	1397:1410	The modularity of this bioink platform design	1397:1441	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
29939754	4	72	theme	UV-induced	796:805	arg1	mechanism					831:839	a rapid UV-induced thiol-ene cross-linking mechanism	788:839	a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation	788:884	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	5	73	theme	divalent	1109:1116	arg1	ions					1118:1121	divalent ions	1109:1121	divalent ions such as calcium	1109:1137	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	5	73	theme	divalent	1109:1116	arg1	calcium					1131:1137	calcium	1131:1137	calcium	1131:1137	The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium.					
29939754	4	74	theme	cross-linking	817:829	arg1	mechanism					831:839	a rapid UV-induced thiol-ene cross-linking mechanism	788:839	a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation	788:884	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	1	75	theme	3D	138:139	arg1	deposition					141:150	precise and controlled 3D deposition	115:150	precise and controlled 3D deposition of biomaterials	115:166	Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner.					
29939754	8	76	theme	similar	1722:1728	arg1	platform					1737:1744	a chemically similar bioink platform	1709:1744	a chemically similar bioink platform	1709:1744	This modularity enables the creation of multizonal and multicellular constructs utilizing a chemically similar bioink platform.					
29939754	2	77	theme	multiple	334:341	arg1	cells					343:347	multiple cells	334:347	multiple cells	334:347	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	9	78	theme	3D	1816:1817	arg1	constructs					1830:1839	3D bioprinted constructs	1816:1839	3D bioprinted constructs	1816:1839	Such tailorable bioink platforms will enable increased complexity in 3D bioprinted constructs.					
29939754	2	79	theme	bioink	247:252	arg1	hydrogel					236:243	Cell-laden hydrogel	225:243	Cell-laden hydrogel ("bioink") bioprinting	225:266	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	2	79	theme	bioink	247:252	arg1	"					253:253	"bioink"	246:253	"bioink"	246:253	Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures.					
29939754	4	80	theme	alginate	711:718	arg1	system					720:725	a norbornene functionalized alginate system	683:725	a norbornene functionalized alginate system	683:725	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	9	81	theme	bioprinted	1819:1828	arg1	constructs					1830:1839	3D bioprinted constructs	1816:1839	3D bioprinted constructs	1816:1839	Such tailorable bioink platforms will enable increased complexity in 3D bioprinted constructs.					
29939754	6	82	theme	in	1218:1219	arg1	gelation					1226:1233	their rapid in situ gelation	1206:1233	their rapid in situ gelation	1206:1233	The low concentrations of alginate needed (<2 wt %), coupled with their rapid in situ gelation, allow both the maintenance of high cell viability and the ability to fabricate large multilayer or multibioink constructs with identical bioprinting conditions.					
29939754	4	83	theme	functionalized	696:709	arg1	system					720:725	a norbornene functionalized alginate system	683:725	a norbornene functionalized alginate system	683:725	In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol-ene cross-linking mechanism that avoids acrylate kinetic chain formation.					
29939754	7	84	theme	bioink	1420:1425	arg1	design					1436:1441	this bioink platform design	1415:1441	this bioink platform design	1415:1441	The modularity of this bioink platform design enables not only the rational design of materials properties but also the gel's biofunctionality (as shown via RGD attachment) for the expected tissue-engineering application.					
31402407	8	0	theme	pectins	1142:1148	arg1	presence					1105:1112	The presence	1101:1112	The presence of highly methyl-esterified pectins in the cortical parenchyma	1101:1175	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	7	1	theme	wall	976:979	arg1	thickenings					981:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	7	1	theme	wall	976:979	arg1	phi					1004:1006	phi	1004:1006	phi	1004:1006	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	8	2	from	presence	1105:1112	arg1	parenchyma					1166:1175	the cortical parenchyma	1153:1175	the cortical parenchyma	1153:1175	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	0	3	theme	Orchidaceae	69:79	arg1	roots					50:54	velamentous roots	38:54	velamentous roots of epiphytic Orchidaceae	38:79	Chemical composition of cell walls in velamentous roots of epiphytic Orchidaceae.					
31402407	0	4	from	composition	9:19	arg1	roots					50:54	velamentous roots	38:54	velamentous roots of epiphytic Orchidaceae	38:79	Chemical composition of cell walls in velamentous roots of epiphytic Orchidaceae.					
31402407	1	5	from	permeability	148:159	arg1	tissues					181:187	root tissues	176:187	root tissues	176:187	The chemical composition of the cell walls strongly affects water permeability and storage in root tissues.					
31402407	3	6	theme	epiphytic	478:486	arg1	species					488:494	18 epiphytic species	475:494	18 epiphytic species belonging to seven subtribes across the Orchidaceae	475:546	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	7	7	theme	desiccation	1088:1098	arg1	periods					1077:1083	periods	1077:1083	periods of desiccation	1077:1098	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	7	8	theme	parenchyma	955:964	arg1	thickenings					981:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	7	8	theme	parenchyma	955:964	arg1	phi					1004:1006	phi	1004:1006	phi	1004:1006	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	4	9	theme	lignin	569:574	arg1	impregnation					553:564	The impregnation	549:564	The impregnation of lignin in the velamen cells	549:595	The impregnation of lignin in the velamen cells reinforces its function as mechanical support and can facilitate apoplastic flow.					
31402407	6	10	contain	possess	826:832	arg2	barriers					909:916	selective barriers	899:916	selective barriers to apoplastic flow	899:935	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	6	10	contain	possess	826:832	arg1	endodermis					815:824	endodermis	815:824	endodermis	815:824	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	6	10	contain	possess	826:832	arg2	walls					876:880	a suberinized lamella and often lignified walls	834:880	a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow	834:935	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	6	10	contain	possess	826:832	arg1	exodermis					801:809	The exodermis	797:809	The exodermis	797:809	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	9	11	from	cells	1339:1343	arg1	parenchyma					1395:1404	the cortical parenchyma	1382:1404	the cortical parenchyma	1382:1404	Finally, cells with lipid or lignin impregnation in the cortical parenchyma could increase the water flow towards the stele.					
31402407	3	12	theme	walls	450:454	arg1	composition					426:436	the composition	422:436	the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae	422:546	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	9	13	theme	water	1425:1429	arg1	flow					1431:1434	the water flow	1421:1434	the water flow	1421:1434	Finally, cells with lipid or lignin impregnation in the cortical parenchyma could increase the water flow towards the stele.					
31402407	2	14	from	live	214:217	arg1	habitat					224:230	a habitat	222:230	a habitat with a highly fluctuating water supply	222:269	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	3	15	theme	present	327:333	arg1	study					335:339	the present study	323:339	the present study	323:339	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	9	16	with	cells	1339:1343	arg1	lipid					1350:1354	lipid	1350:1354	lipid	1350:1354	Finally, cells with lipid or lignin impregnation in the cortical parenchyma could increase the water flow towards the stele.					
31402407	9	16	with	cells	1339:1343	arg1	impregnation					1366:1377	lignin impregnation	1359:1377	lignin impregnation	1359:1377	Finally, cells with lipid or lignin impregnation in the cortical parenchyma could increase the water flow towards the stele.					
31402407	2	17	theme	water	258:262	arg1	supply					264:269	a highly fluctuating water supply	237:269	a highly fluctuating water supply	237:269	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	2	18	from	habitat	224:230	arg1	live					214:217	live	214:217	live	214:217	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	3	19	theme	histochemistry	350:363	arg1	techniques					389:398	histochemistry and immunocytochemistry techniques	350:398	histochemistry and immunocytochemistry techniques	350:398	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	8	20	theme	methyl-esterified	1124:1140	arg1	pectins					1142:1148	highly methyl-esterified pectins	1117:1148	highly methyl-esterified pectins	1117:1148	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	5	21	dep	stability	746:754	arg1	the					742:744	the	742:744	the	742:744	Pectins, as well cellulose and lignins, are also essential for the stability and mechanical support of velamen cells.					
31402407	6	22	theme	lignified	866:874	arg1	barriers					909:916	selective barriers	899:916	selective barriers to apoplastic flow	899:935	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	6	22	theme	lignified	866:874	arg1	walls					876:880	a suberinized lamella and often lignified walls	834:880	a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow	834:935	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	1	23	theme	root	176:179	arg1	tissues					181:187	root tissues	176:187	root tissues	176:187	The chemical composition of the cell walls strongly affects water permeability and storage in root tissues.					
31402407	0	24	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of cell walls in velamentous roots of epiphytic Orchidaceae.	0:80	Chemical composition of cell walls in velamentous roots of epiphytic Orchidaceae.					
31402407	8	25	theme	cortical	1157:1164	arg1	parenchyma					1166:1175	the cortical parenchyma	1153:1175	the cortical parenchyma	1153:1175	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	1	26	theme	chemical	86:93	arg1	composition					95:105	The chemical composition	82:105	The chemical composition of the cell walls	82:123	The chemical composition of the cell walls strongly affects water permeability and storage in root tissues.					
31402407	8	27	theme	increased	1239:1247	arg1	porosity					1249:1256	increased porosity	1239:1256	increased porosity	1239:1256	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	0	28	theme	cell	24:27	arg1	walls					29:33	cell walls	24:33	cell walls	24:33	Chemical composition of cell walls in velamentous roots of epiphytic Orchidaceae.					
31402407	5	29	theme	velamen	782:788	arg1	cells					790:794	velamen cells	782:794	velamen cells	782:794	Pectins, as well cellulose and lignins, are also essential for the stability and mechanical support of velamen cells.					
31402407	3	30	used	used	345:348	arg2	we					342:343	we	342:343	we	342:343	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	7	31	theme	Various	938:944	arg1	thickenings					981:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	7	31	theme	Various	938:944	arg1	phi					1004:1006	phi	1004:1006	phi	1004:1006	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	2	32	theme	cell	281:284	arg1	walls					286:290	the root cell walls	272:290	the root cell walls	272:290	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	2	32	theme	cell	281:284	arg1	important					309:317	important	309:317	important	309:317	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	7	33	theme	cortical	946:953	arg1	thickenings					981:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	7	33	theme	cortical	946:953	arg1	phi					1004:1006	phi	1004:1006	phi	1004:1006	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	3	34	theme	cell	445:448	arg1	walls					450:454	the cell walls	441:454	the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae	441:546	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	2	35	with	habitat	224:230	arg1	supply					264:269	a highly fluctuating water supply	237:269	a highly fluctuating water supply	237:269	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	6	36	theme	lamella	848:854	arg1	barriers					909:916	selective barriers	899:916	selective barriers to apoplastic flow	899:935	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	6	36	theme	lamella	848:854	arg1	walls					876:880	a suberinized lamella and often lignified walls	834:880	a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow	834:935	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	2	37	theme	root	276:279	arg1	walls					286:290	the root cell walls	272:290	the root cell walls	272:290	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	2	37	theme	root	276:279	arg1	important					309:317	important	309:317	important	309:317	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	2	38	dep	live	214:217	arg1	orchids					206:212	epiphytic orchids	196:212	epiphytic orchids	196:212	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	8	39	theme	water	1280:1284	arg1	storage					1286:1292	water storage	1280:1292	water storage	1280:1292	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	6	40	theme	suberinized	836:846	arg1	lamella					848:854	a suberinized lamella	834:854	a suberinized lamella	834:854	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	4	41	theme	mechanical	624:633	arg1	support					635:641	mechanical support	624:641	mechanical support	624:641	The impregnation of lignin in the velamen cells reinforces its function as mechanical support and can facilitate apoplastic flow.					
31402407	0	42	theme	walls	29:33	arg1	composition					9:19	Chemical composition	0:19	Chemical composition of cell walls in velamentous roots of epiphytic Orchidaceae.	0:80	Chemical composition of cell walls in velamentous roots of epiphytic Orchidaceae.					
31402407	8	43	theme	gels	1206:1209	arg1	formation					1193:1201	the formation	1189:1201	the formation of gels	1189:1209	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	5	44	theme	cells	790:794	arg1	stability					746:754	stability	746:754	stability	746:754	Pectins, as well cellulose and lignins, are also essential for the stability and mechanical support of velamen cells.					
31402407	5	44	theme	cells	790:794	arg1	support					771:777	mechanical support	760:777	mechanical support	760:777	Pectins, as well cellulose and lignins, are also essential for the stability and mechanical support of velamen cells.					
31402407	4	45	from	impregnation	553:564	arg1	cells					591:595	the velamen cells	579:595	the velamen cells	579:595	The impregnation of lignin in the velamen cells reinforces its function as mechanical support and can facilitate apoplastic flow.					
31402407	3	46	theme	immunocytochemistry	369:387	arg1	techniques					389:398	histochemistry and immunocytochemistry techniques	350:398	histochemistry and immunocytochemistry techniques	350:398	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	6	47	theme	apoplastic	921:930	arg1	flow					932:935	apoplastic flow	921:935	apoplastic flow	921:935	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	7	48	theme	uniform	1026:1032	arg1	thickenings					981:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	7	48	theme	uniform	1026:1032	arg1	phi					1004:1006	phi	1004:1006	phi	1004:1006	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	7	49	theme	reticulated	1009:1019	arg1	thickenings					981:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	7	49	theme	reticulated	1009:1019	arg1	phi					1004:1006	phi	1004:1006	phi	1004:1006	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	3	50	theme	tissues	464:470	arg1	walls					450:454	the cell walls	441:454	the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae	441:546	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	0	51	theme	velamentous	38:48	arg1	roots					50:54	velamentous roots	38:54	velamentous roots of epiphytic Orchidaceae	38:79	Chemical composition of cell walls in velamentous roots of epiphytic Orchidaceae.					
31402407	9	52	theme	lignin	1359:1364	arg1	impregnation					1366:1377	lignin impregnation	1359:1377	lignin impregnation	1359:1377	Finally, cells with lipid or lignin impregnation in the cortical parenchyma could increase the water flow towards the stele.					
31402407	1	53	theme	cell	114:117	arg1	walls					119:123	the cell walls	110:123	the cell walls	110:123	The chemical composition of the cell walls strongly affects water permeability and storage in root tissues.					
31402407	2	54	theme	epiphytic	196:204	arg1	orchids					206:212	epiphytic orchids	196:212	epiphytic orchids	196:212	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	2	55	theme	fluctuating	246:256	arg1	supply					264:269	a highly fluctuating water supply	237:269	a highly fluctuating water supply	237:269	Since epiphytic orchids live in a habitat with a highly fluctuating water supply, the root cell walls are functionally important.					
31402407	4	56	theme	apoplastic	662:671	arg1	flow					673:676	apoplastic flow	662:676	apoplastic flow	662:676	The impregnation of lignin in the velamen cells reinforces its function as mechanical support and can facilitate apoplastic flow.					
31402407	1	57	theme	walls	119:123	arg1	composition					95:105	The chemical composition	82:105	The chemical composition of the cell walls	82:123	The chemical composition of the cell walls strongly affects water permeability and storage in root tissues.					
31402407	7	58	theme	secondary	966:974	arg1	thickenings					981:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings	938:991	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	7	58	theme	secondary	966:974	arg1	phi					1004:1006	phi	1004:1006	phi	1004:1006	Various cortical parenchyma secondary wall thickenings, including phi, reticulated, and uniform, prevent the cortex from collapsing during periods of desiccation.					
31402407	4	59	theme	velamen	583:589	arg1	cells					591:595	the velamen cells	579:595	the velamen cells	579:595	The impregnation of lignin in the velamen cells reinforces its function as mechanical support and can facilitate apoplastic flow.					
31402407	9	60	theme	cortical	1386:1393	arg1	parenchyma					1395:1404	the cortical parenchyma	1382:1404	the cortical parenchyma	1382:1404	Finally, cells with lipid or lignin impregnation in the cortical parenchyma could increase the water flow towards the stele.					
31402407	3	61	theme	root	459:462	arg1	tissues					464:470	root tissues	459:470	root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae	459:546	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	8	62	theme	wall	1220:1223	arg1	loosening					1225:1233	wall loosening	1220:1233	wall loosening	1220:1233	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	8	63	theme	solute	1298:1303	arg1	transport					1305:1313	solute transport	1298:1313	solute transport	1298:1313	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	0	64	theme	epiphytic	59:67	arg1	Orchidaceae					69:79	epiphytic Orchidaceae	59:79	epiphytic Orchidaceae	59:79	Chemical composition of cell walls in velamentous roots of epiphytic Orchidaceae.					
31402407	3	65	theme	species	488:494	arg1	tissues					464:470	root tissues	459:470	root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae	459:546	In the present study, we used histochemistry and immunocytochemistry techniques in order to determine the composition of the cell walls of root tissues of 18 epiphytic species belonging to seven subtribes across the Orchidaceae.					
31402407	8	66	attach	presence	1105:1112	arg2	pectins					1142:1148	highly methyl-esterified pectins	1117:1148	highly methyl-esterified pectins	1117:1148	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	8	66	attach	presence	1105:1112	arg1	parenchyma					1166:1175	the cortical parenchyma	1153:1175	the cortical parenchyma	1153:1175	The presence of highly methyl-esterified pectins in the cortical parenchyma facilitates the formation of gels, causing wall loosening and increased porosity, which contributes to water storage and solute transport between cells.					
31402407	5	67	theme	mechanical	760:769	arg1	support					771:777	mechanical support	760:777	mechanical support	760:777	Pectins, as well cellulose and lignins, are also essential for the stability and mechanical support of velamen cells.					
31402407	6	68	theme	selective	899:907	arg1	barriers					909:916	selective barriers	899:916	selective barriers to apoplastic flow	899:935	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	6	68	theme	selective	899:907	arg1	walls					876:880	a suberinized lamella and often lignified walls	834:880	a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow	834:935	The exodermis and endodermis possess a suberinized lamella and often lignified walls that function as selective barriers to apoplastic flow.					
31402407	1	69	from	storage	165:171	arg1	tissues					181:187	root tissues	176:187	root tissues	176:187	The chemical composition of the cell walls strongly affects water permeability and storage in root tissues.					
31402407	1	70	theme	water	142:146	arg1	permeability					148:159	water permeability	142:159	water permeability	142:159	The chemical composition of the cell walls strongly affects water permeability and storage in root tissues.					
31556338	8	0	theme	MgSt	1157:1160	arg1	performance					1162:1172	MgSt performance	1157:1172	MgSt performance	1157:1172	It was found that the prediction of MgSt performance from its properties greatly depends on filler type and PAs of interest.					
31556338	6	1	theme	formulation	921:931	arg1	parameters					945:954	The key formulation and process parameters	913:954	The key formulation and process parameters	913:954	The key formulation and process parameters include MgSt concentration, filler type and compression force.					
31556338	2	2	from	measurement	399:409	arg1	prediction					443:452	prediction	443:452	prediction of performance in tablet direct compression	443:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	10	3	theme	contributing	1405:1416	arg1	properties					1433:1442	the contributing physiochemical properties	1401:1442	the contributing physiochemical properties in performance prediction	1401:1468	In the cases that the contributing physiochemical properties in performance prediction are sufficiently captured in the spectra, the spectra can be used as an alternative tool to predict excipient performance.					
31556338	3	4	theme	square	513:518	arg1	models					520:525	Partial least square models	499:525	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra	499:597	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra were developed and validated.					
31556338	4	5	theme	MgSt	659:662	arg1	variables					645:653	The model input variables	629:653	The model input variables	629:653	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	4	5	theme	MgSt	659:662	arg1	properties					679:688	MgSt physiochemical properties	659:688	MgSt physiochemical properties	659:688	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	10	6	theme	performance	1447:1457	arg1	prediction					1459:1468	performance prediction	1447:1468	performance prediction	1447:1468	In the cases that the contributing physiochemical properties in performance prediction are sufficiently captured in the spectra, the spectra can be used as an alternative tool to predict excipient performance.					
31556338	3	7	theme	performance	538:548	arg1	properties					577:586	performance attributes (PAs) from MgSt properties	538:586	performance attributes (PAs) from MgSt properties	538:586	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra were developed and validated.					
31556338	7	8	theme	disintegration	1100:1113	arg1	time					1115:1118	disintegration time	1100:1118	disintegration time	1100:1118	The output variables are PAs including tablet ejection force, breaking force and disintegration time.					
31556338	3	9	theme	MgSt	572:575	arg1	properties					577:586	performance attributes (PAs) from MgSt properties	538:586	performance attributes (PAs) from MgSt properties	538:586	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra were developed and validated.					
31556338	8	10	theme	filler	1213:1218	arg1	type					1220:1223	filler type	1213:1223	filler type	1213:1223	It was found that the prediction of MgSt performance from its properties greatly depends on filler type and PAs of interest.					
31556338	6	11	theme	filler	984:989	arg1	type					991:994	filler type	984:994	filler type	984:994	The key formulation and process parameters include MgSt concentration, filler type and compression force.					
31556338	4	12	theme	model	633:637	arg1	variables					645:653	The model input variables	629:653	The model input variables	629:653	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	4	12	theme	model	633:637	arg1	properties					679:688	MgSt physiochemical properties	659:688	MgSt physiochemical properties	659:688	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	1	13	theme	stearate	204:211	arg1	properties					180:189	the properties	176:189	the properties of magnesium stearate (MgSt)	176:218	The objective of this research is to understand how the properties of magnesium stearate (MgSt) affect product performance in a quantitative manner using a multivariate modeling approach.					
31556338	9	14	theme	NIR	1246:1248	arg1	spectra					1250:1256	NIR spectra	1246:1256	NIR spectra	1246:1256	NIR spectra successfully predicted lubricant performance in lactose tablet; however, predictions from Raman spectra were not acceptable.					
31556338	5	15	theme	specific	862:869	arg1	area					879:882	specific surface area	862:882	specific surface area	862:882	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	7	16	theme	ejection	1065:1072	arg1	force					1074:1078	tablet ejection force	1058:1078	tablet ejection force	1058:1078	The output variables are PAs including tablet ejection force, breaking force and disintegration time.					
31556338	6	17	theme	process	937:943	arg1	parameters					945:954	The key formulation and process parameters	913:954	The key formulation and process parameters	913:954	The key formulation and process parameters include MgSt concentration, filler type and compression force.					
31556338	7	18	theme	output	1023:1028	arg1	PAs					1044:1046	PAs	1044:1046	PAs including tablet ejection force, breaking force and disintegration time	1044:1118	The output variables are PAs including tablet ejection force, breaking force and disintegration time.					
31556338	7	18	theme	output	1023:1028	arg1	variables					1030:1038	The output variables	1019:1038	The output variables	1019:1038	The output variables are PAs including tablet ejection force, breaking force and disintegration time.					
31556338	9	19	theme	lubricant	1281:1289	arg1	performance					1291:1301	lubricant performance	1281:1301	lubricant performance in lactose tablet	1281:1319	NIR spectra successfully predicted lubricant performance in lactose tablet; however, predictions from Raman spectra were not acceptable.					
31556338	5	20	theme	fatty	783:787	arg1	composition					794:804	fatty acid composition	783:804	fatty acid composition	783:804	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	5	21	theme	state	895:899	arg1	properties					901:910	solid state properties	889:910	solid state properties	889:910	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	0	22	from	impact	18:23	arg1	performance					72:82	tableting performance	62:82	tableting performance using a multivariate modeling approach	62:121	Understanding the impact of magnesium stearate variability on tableting performance using a multivariate modeling approach.					
31556338	4	23	theme	key	700:702	arg1	formulation					704:714	key formulation	700:714	key formulation	700:714	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	5	24	from	properties	901:910	arg1	drying					815:820	drying	815:820	drying	815:820	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	0	25	theme	multivariate	92:103	arg1	approach					114:121	a multivariate modeling approach	90:121	a multivariate modeling approach	90:121	Understanding the impact of magnesium stearate variability on tableting performance using a multivariate modeling approach.					
31556338	10	26	used	used	1531:1534	arg2	tool					1554:1557	an alternative tool	1539:1557	an alternative tool to predict excipient performance	1539:1590	In the cases that the contributing physiochemical properties in performance prediction are sufficiently captured in the spectra, the spectra can be used as an alternative tool to predict excipient performance.					
31556338	10	26	used	used	1531:1534	arg2	spectra					1516:1522	the spectra	1512:1522	the spectra	1512:1522	In the cases that the contributing physiochemical properties in performance prediction are sufficiently captured in the spectra, the spectra can be used as an alternative tool to predict excipient performance.					
31556338	2	27	theme	Raman	370:374	arg1	spectra					376:382	NIR and Raman spectra	362:382	spectra	376:382	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	2	27	theme	Raman	370:374	arg1	measurement					399:409	a surrogate measurement	387:409	a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression	387:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	9	28	theme	Raman	1348:1352	arg1	spectra					1354:1360	Raman spectra	1348:1360	Raman spectra	1348:1360	NIR spectra successfully predicted lubricant performance in lactose tablet; however, predictions from Raman spectra were not acceptable.					
31556338	9	29	from	spectra	1354:1360	arg1	predictions					1331:1341	predictions	1331:1341	predictions from Raman spectra	1331:1360	NIR spectra successfully predicted lubricant performance in lactose tablet; however, predictions from Raman spectra were not acceptable.					
31556338	2	30	theme	properties	429:438	arg1	spectra					376:382	NIR and Raman spectra	362:382	spectra	376:382	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	2	30	theme	properties	429:438	arg1	measurement					399:409	a surrogate measurement	387:409	a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression	387:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	5	31	theme	particle	834:841	arg1	distribution					848:859	particle size distribution	834:859	particle size distribution	834:859	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	1	32	theme	multivariate	280:291	arg1	approach					302:309	a multivariate modeling approach	278:309	a multivariate modeling approach	278:309	The objective of this research is to understand how the properties of magnesium stearate (MgSt) affect product performance in a quantitative manner using a multivariate modeling approach.					
31556338	1	33	theme	research	146:153	arg1	objective					128:136	The objective	124:136	The objective of this research	124:153	The objective of this research is to understand how the properties of magnesium stearate (MgSt) affect product performance in a quantitative manner using a multivariate modeling approach.					
31556338	2	34	theme	tablet	472:477	arg1	compression					486:496	tablet direct compression	472:496	tablet direct compression	472:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	2	35	theme	surrogate	389:397	arg1	spectra					376:382	NIR and Raman spectra	362:382	spectra	376:382	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	2	35	theme	surrogate	389:397	arg1	measurement					399:409	a surrogate measurement	387:409	a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression	387:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	0	36	theme	magnesium	28:36	arg1	variability					47:57	magnesium stearate variability	28:57	magnesium stearate variability	28:57	Understanding the impact of magnesium stearate variability on tableting performance using a multivariate modeling approach.					
31556338	2	37	theme	performance	457:467	arg1	prediction					443:452	prediction	443:452	prediction of performance in tablet direct compression	443:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	10	38	theme	alternative	1542:1552	arg1	tool					1554:1557	an alternative tool	1539:1557	an alternative tool to predict excipient performance	1539:1590	In the cases that the contributing physiochemical properties in performance prediction are sufficiently captured in the spectra, the spectra can be used as an alternative tool to predict excipient performance.					
31556338	10	38	theme	alternative	1542:1552	arg1	spectra					1516:1522	the spectra	1512:1522	the spectra	1512:1522	In the cases that the contributing physiochemical properties in performance prediction are sufficiently captured in the spectra, the spectra can be used as an alternative tool to predict excipient performance.					
31556338	0	39	theme	tableting	62:70	arg1	performance					72:82	tableting performance	62:82	tableting performance using a multivariate modeling approach	62:121	Understanding the impact of magnesium stearate variability on tableting performance using a multivariate modeling approach.					
31556338	0	40	theme	variability	47:57	arg1	impact					18:23	the impact	14:23	the impact of magnesium stearate variability on tableting performance using a multivariate modeling approach	14:121	Understanding the impact of magnesium stearate variability on tableting performance using a multivariate modeling approach.					
31556338	8	41	from	properties	1183:1192	arg1	prediction					1143:1152	the prediction	1139:1152	the prediction of MgSt performance from its properties	1139:1192	It was found that the prediction of MgSt performance from its properties greatly depends on filler type and PAs of interest.					
31556338	10	42	theme	excipient	1570:1578	arg1	performance					1580:1590	excipient performance	1570:1590	excipient performance	1570:1590	In the cases that the contributing physiochemical properties in performance prediction are sufficiently captured in the spectra, the spectra can be used as an alternative tool to predict excipient performance.					
31556338	2	43	theme	NIR	362:364	arg1	spectra					376:382	NIR and Raman spectra	362:382	spectra	376:382	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	2	43	theme	NIR	362:364	arg1	measurement					399:409	a surrogate measurement	387:409	a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression	387:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	1	44	theme	product	227:233	arg1	performance					235:245	product performance	227:245	product performance	227:245	The objective of this research is to understand how the properties of magnesium stearate (MgSt) affect product performance in a quantitative manner using a multivariate modeling approach.					
31556338	9	45	from	performance	1291:1301	arg1	tablet					1314:1319	lactose tablet	1306:1319	lactose tablet	1306:1319	NIR spectra successfully predicted lubricant performance in lactose tablet; however, predictions from Raman spectra were not acceptable.					
31556338	6	46	theme	key	917:919	arg1	parameters					945:954	The key formulation and process parameters	913:954	The key formulation and process parameters	913:954	The key formulation and process parameters include MgSt concentration, filler type and compression force.					
31556338	3	47	theme	attributes	550:559	arg1	properties					577:586	performance attributes (PAs) from MgSt properties	538:586	performance attributes (PAs) from MgSt properties	538:586	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra were developed and validated.					
31556338	1	48	theme	quantitative	252:263	arg1	manner					265:270	a quantitative manner	250:270	a quantitative manner using a multivariate modeling approach	250:309	The objective of this research is to understand how the properties of magnesium stearate (MgSt) affect product performance in a quantitative manner using a multivariate modeling approach.					
31556338	8	49	theme	performance	1162:1172	arg1	prediction					1143:1152	the prediction	1139:1152	the prediction of MgSt performance from its properties	1139:1192	It was found that the prediction of MgSt performance from its properties greatly depends on filler type and PAs of interest.					
31556338	5	50	theme	surface	871:877	arg1	area					879:882	specific surface area	862:882	specific surface area	862:882	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	8	51	theme	interest	1236:1243	arg1	PAs					1229:1231	PAs	1229:1231	PAs	1229:1231	It was found that the prediction of MgSt performance from its properties greatly depends on filler type and PAs of interest.					
31556338	8	51	theme	interest	1236:1243	arg1	type					1220:1223	filler type	1213:1223	filler type	1213:1223	It was found that the prediction of MgSt performance from its properties greatly depends on filler type and PAs of interest.					
31556338	3	52	theme	 from	566:570	arg1	properties					577:586	performance attributes (PAs) from MgSt properties	538:586	performance attributes (PAs) from MgSt properties	538:586	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra were developed and validated.					
31556338	4	53	theme	physiochemical	664:677	arg1	variables					645:653	The model input variables	629:653	The model input variables	629:653	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	4	53	theme	physiochemical	664:677	arg1	properties					679:688	MgSt physiochemical properties	659:688	MgSt physiochemical properties	659:688	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	7	54	theme	breaking	1081:1088	arg1	force					1090:1094	breaking force	1081:1094	breaking force	1081:1094	The output variables are PAs including tablet ejection force, breaking force and disintegration time.					
31556338	10	55	theme	physiochemical	1418:1431	arg1	properties					1433:1442	the contributing physiochemical properties	1401:1442	the contributing physiochemical properties in performance prediction	1401:1468	In the cases that the contributing physiochemical properties in performance prediction are sufficiently captured in the spectra, the spectra can be used as an alternative tool to predict excipient performance.					
31556338	3	56	theme	PAs	562:564	arg1	properties					577:586	performance attributes (PAs) from MgSt properties	538:586	performance attributes (PAs) from MgSt properties	538:586	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra were developed and validated.					
31556338	1	57	theme	magnesium	194:202	arg1	stearate					204:211	magnesium stearate	194:211	magnesium stearate (MgSt)	194:218	The objective of this research is to understand how the properties of magnesium stearate (MgSt) affect product performance in a quantitative manner using a multivariate modeling approach.					
31556338	1	57	theme	magnesium	194:202	arg1	MgSt					214:217	MgSt	214:217	MgSt	214:217	The objective of this research is to understand how the properties of magnesium stearate (MgSt) affect product performance in a quantitative manner using a multivariate modeling approach.					
31556338	2	58	from	compression	486:496	arg1	prediction					443:452	prediction	443:452	prediction of performance in tablet direct compression	443:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	6	59	theme	MgSt	964:967	arg1	concentration					969:981	MgSt concentration	964:981	MgSt concentration	964:981	The key formulation and process parameters include MgSt concentration, filler type and compression force.					
31556338	4	60	theme	input	639:643	arg1	variables					645:653	The model input variables	629:653	The model input variables	629:653	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	4	60	theme	input	639:643	arg1	properties					679:688	MgSt physiochemical properties	659:688	MgSt physiochemical properties	659:688	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	7	61	theme	tablet	1058:1063	arg1	force					1074:1078	tablet ejection force	1058:1078	tablet ejection force	1058:1078	The output variables are PAs including tablet ejection force, breaking force and disintegration time.					
31556338	5	62	from	composition	794:804	arg1	drying					815:820	drying	815:820	drying	815:820	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	5	63	from	distribution	848:859	arg1	drying					815:820	drying	815:820	drying	815:820	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	4	64	theme	process	720:726	arg1	parameters					728:737	process parameters	720:737	process parameters	720:737	The model input variables are MgSt physiochemical properties, spectra, key formulation and process parameters.					
31556338	5	65	theme	size	843:846	arg1	distribution					848:859	particle size distribution	834:859	particle size distribution	834:859	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	5	66	theme	solid	889:893	arg1	properties					901:910	solid state properties	889:910	solid state properties	889:910	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	2	67	from	properties	429:438	arg1	prediction					443:452	prediction	443:452	prediction of performance in tablet direct compression	443:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	5	68	theme	Material	740:747	arg1	properties					764:773	Material physiochemical properties	740:773	Material physiochemical properties	740:773	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	5	69	theme	acid	789:792	arg1	composition					794:804	fatty acid composition	783:804	fatty acid composition	783:804	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	9	70	theme	lactose	1306:1312	arg1	tablet					1314:1319	lactose tablet	1306:1319	lactose tablet	1306:1319	NIR spectra successfully predicted lubricant performance in lactose tablet; however, predictions from Raman spectra were not acceptable.					
31556338	5	71	from	area	879:882	arg1	drying					815:820	drying	815:820	drying	815:820	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	2	72	theme	direct	479:484	arg1	compression					486:496	tablet direct compression	472:496	tablet direct compression	472:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	2	73	from	prediction	443:452	arg1	spectra					376:382	NIR and Raman spectra	362:382	spectra	376:382	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	2	73	from	prediction	443:452	arg1	measurement					399:409	a surrogate measurement	387:409	a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression	387:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	2	73	from	prediction	443:452	arg1	compression					486:496	tablet direct compression	472:496	tablet direct compression	472:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	0	74	theme	modeling	105:112	arg1	approach					114:121	a multivariate modeling approach	90:121	a multivariate modeling approach	90:121	Understanding the impact of magnesium stearate variability on tableting performance using a multivariate modeling approach.					
31556338	5	75	from	loss	807:810	arg1	drying					815:820	drying	815:820	drying	815:820	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	6	76	theme	compression	1000:1010	arg1	force					1012:1016	compression force	1000:1016	compression force	1000:1016	The key formulation and process parameters include MgSt concentration, filler type and compression force.					
31556338	2	77	from	performance	457:467	arg1	compression					486:496	tablet direct compression	472:496	tablet direct compression	472:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	5	78	theme	physiochemical	749:762	arg1	properties					764:773	Material physiochemical properties	740:773	Material physiochemical properties	740:773	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	5	79	from	densities	823:831	arg1	drying					815:820	drying	815:820	drying	815:820	Material physiochemical properties include fatty acid composition, loss on drying, densities, particle size distribution, specific surface area, and solid state properties.					
31556338	3	80	dep	models	520:525	arg1	predict					530:536	predict	530:536	to predict performance attributes (PAs) from MgSt properties or spectra	527:597	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra were developed and validated.					
31556338	2	81	theme	physiochemical	414:427	arg1	properties					429:438	physiochemical properties	414:438	physiochemical properties in prediction of performance in tablet direct compression	414:496	In addition, we explored the feasibility of using NIR and Raman spectra as a surrogate measurement of physiochemical properties in prediction of performance in tablet direct compression.					
31556338	0	82	theme	stearate	38:45	arg1	variability					47:57	magnesium stearate variability	28:57	magnesium stearate variability	28:57	Understanding the impact of magnesium stearate variability on tableting performance using a multivariate modeling approach.					
31556338	1	83	theme	modeling	293:300	arg1	approach					302:309	a multivariate modeling approach	278:309	a multivariate modeling approach	278:309	The objective of this research is to understand how the properties of magnesium stearate (MgSt) affect product performance in a quantitative manner using a multivariate modeling approach.					
31556338	3	84	theme	Partial	499:505	arg1	models					520:525	Partial least square models	499:525	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra	499:597	Partial least square models to predict performance attributes (PAs) from MgSt properties or spectra were developed and validated.					
31556338	10	85	from	properties	1433:1442	arg1	prediction					1459:1468	performance prediction	1447:1468	performance prediction	1447:1468	In the cases that the contributing physiochemical properties in performance prediction are sufficiently captured in the spectra, the spectra can be used as an alternative tool to predict excipient performance.					
30566463	0	0	theme	HA/collagen	80:90	arg1	scaffolds					92:100	3D hybrid HA/collagen scaffolds	70:100	3D hybrid HA/collagen scaffolds	70:100	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	5	1	theme	chain	801:805	arg1	conformations					807:819	non-equilibrium chain conformations	785:819	non-equilibrium chain conformations	785:819	Most strikingly, local elastic moduli were significantly lower than bulk moduli presumably due to non-equilibrium chain conformations between crosslinks.					
30566463	7	2	theme	elastic	918:924	arg1	moduli					926:931	Local elastic moduli	912:931	Local elastic moduli	912:931	Local elastic moduli were similar for all gels, irrespective of their different swelling ratio and bulk moduli.					
30566463	4	3	theme	homogeneous	602:612	arg1	networks					614:621	homogeneous networks	602:621	homogeneous networks	602:621	Both one-component gels formed homogeneous networks, whereas hybrid gels were heterogeneous in terms of elasticity.					
30566463	0	4	theme	hybrid	73:78	arg1	scaffolds					92:100	3D hybrid HA/collagen scaffolds	70:100	3D hybrid HA/collagen scaffolds	70:100	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	7	5	theme	different	982:990	arg1	ratio					1001:1005	their different swelling ratio and bulk moduli	976:1021	ratio	1001:1005	Local elastic moduli were similar for all gels, irrespective of their different swelling ratio and bulk moduli.					
30566463	7	6	theme	bulk	1011:1014	arg1	moduli					1016:1021	their different swelling ratio and bulk moduli	976:1021	moduli	1016:1021	Local elastic moduli were similar for all gels, irrespective of their different swelling ratio and bulk moduli.					
30566463	5	7	theme	bulk	755:758	arg1	moduli					760:765	bulk moduli	755:765	bulk moduli	755:765	Most strikingly, local elastic moduli were significantly lower than bulk moduli presumably due to non-equilibrium chain conformations between crosslinks.					
30566463	7	8	theme	swelling	992:999	arg1	ratio					1001:1005	their different swelling ratio and bulk moduli	976:1021	ratio	1001:1005	Local elastic moduli were similar for all gels, irrespective of their different swelling ratio and bulk moduli.					
30566463	0	9	theme	scaffolds	92:100	arg1	Microstructure					0:13	Microstructure	0:13	Microstructure	0:13	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	0	9	theme	scaffolds	92:100	arg1	viscoelasticity					22:36	local viscoelasticity	16:36	local viscoelasticity	16:36	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	0	9	theme	scaffolds	92:100	arg1	suitability					55:65	cell culture suitability	42:65	cell culture suitability	42:65	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	9	10	theme	Coll	1110:1113	arg1	gels					1126:1129	Coll containing gels	1110:1129	Coll containing gels	1110:1129	Coll containing gels enabled cell migration, adhesion and proliferation inside the gels.					
30566463	3	11	theme	acid	521:524	arg1	mixtures					498:505	mixtures	498:505	mixtures of hyaluronic acid (HA) and collagen (Coll)	498:549	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	8	12	theme	investigated	1083:1094	arg1	compositions					1096:1107	all investigated compositions	1079:1107	all investigated compositions	1079:1107	Fibroblast cell culture proved the biocompatibility of all investigated compositions.					
30566463	9	13	theme	containing	1115:1124	arg1	gels					1126:1129	Coll containing gels	1110:1129	Coll containing gels	1110:1129	Coll containing gels enabled cell migration, adhesion and proliferation inside the gels.					
30566463	3	14	theme	three-dimensional	457:473	arg1	macroporous					444:454	macroporous	444:454	macroporous	444:454	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	3	14	theme	three-dimensional	457:473	arg1	scaffolds					483:491	three-dimensional cryogel scaffolds	457:491	three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll)	457:549	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	8	15	theme	Fibroblast	1024:1033	arg1	culture					1040:1046	Fibroblast cell culture	1024:1046	Fibroblast cell culture	1024:1046	Fibroblast cell culture proved the biocompatibility of all investigated compositions.					
30566463	2	16	theme	particle	274:281	arg1	tracking					283:290	multiple particle tracking	265:290	multiple particle tracking	265:290	We used multiple particle tracking microrheology to close the gap between elasticity determined from bulk measurements and elastic properties sensed by cells.					
30566463	2	17	used	used	260:263	arg2	We					257:258	We	257:258	We	257:258	We used multiple particle tracking microrheology to close the gap between elasticity determined from bulk measurements and elastic properties sensed by cells.					
30566463	1	18	theme	mechanical	175:184	arg1	characterization					186:201	mechanical characterization	175:201	mechanical characterization of scaffolds on a relevant length scale	175:241	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	4	19	theme	elasticity	675:684	arg1	terms					666:670	terms	666:670	terms of elasticity	666:684	Both one-component gels formed homogeneous networks, whereas hybrid gels were heterogeneous in terms of elasticity.					
30566463	3	20	theme	macroporous	444:454	arg1	elasticity					430:439	elasticity	430:439	elasticity	430:439	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	3	20	theme	macroporous	444:454	arg1	Structure					416:424	Structure	416:424	Structure	416:424	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	8	21	theme	compositions	1096:1107	arg1	biocompatibility					1059:1074	the biocompatibility	1055:1074	the biocompatibility of all investigated compositions	1055:1107	Fibroblast cell culture proved the biocompatibility of all investigated compositions.					
30566463	9	22	theme	cell	1139:1142	arg1	migration					1144:1152	cell migration	1139:1152	cell migration	1139:1152	Coll containing gels enabled cell migration, adhesion and proliferation inside the gels.					
30566463	6	23	from	pronounced	855:864	arg1	Coll					869:872	Coll	869:872	Coll	869:872	This was more pronounced in Coll and hybrid gels than in pure HA gels.					
30566463	6	23	from	pronounced	855:864	arg1	gels					885:888	hybrid gels	878:888	hybrid gels	878:888	This was more pronounced in Coll and hybrid gels than in pure HA gels.					
30566463	6	23	from	pronounced	855:864	arg1	gels					906:909	pure HA gels	898:909	pure HA gels	898:909	This was more pronounced in Coll and hybrid gels than in pure HA gels.					
30566463	8	24	theme	cell	1035:1038	arg1	culture					1040:1046	Fibroblast cell culture	1024:1046	Fibroblast cell culture	1024:1046	Fibroblast cell culture proved the biocompatibility of all investigated compositions.					
30566463	4	25	from	terms	666:670	arg1	heterogeneous					649:661	heterogeneous	649:661	heterogeneous	649:661	Both one-component gels formed homogeneous networks, whereas hybrid gels were heterogeneous in terms of elasticity.					
30566463	1	26	theme	scaffolds	206:214	arg1	characterization					186:201	mechanical characterization	175:201	mechanical characterization of scaffolds on a relevant length scale	175:241	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	0	27	theme	local	16:20	arg1	Microstructure					0:13	Microstructure	0:13	Microstructure	0:13	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	0	27	theme	local	16:20	arg1	viscoelasticity					22:36	local viscoelasticity	16:36	local viscoelasticity	16:36	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	3	28	from	mixtures	498:505	arg1	macroporous					444:454	macroporous	444:454	macroporous	444:454	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	3	28	from	mixtures	498:505	arg1	scaffolds					483:491	three-dimensional cryogel scaffolds	457:491	three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll)	457:549	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	6	29	theme	HA	903:904	arg1	gels					906:909	pure HA gels	898:909	pure HA gels	898:909	This was more pronounced in Coll and hybrid gels than in pure HA gels.					
30566463	1	30	theme	mechanical	106:115	arg1	properties					117:126	mechanical properties	106:126	mechanical properties of cell culture substrates	106:153	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	3	31	theme	collagen	535:542	arg1	mixtures					498:505	mixtures	498:505	mixtures of hyaluronic acid (HA) and collagen (Coll)	498:549	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	0	32	theme	cell	42:45	arg1	Microstructure					0:13	Microstructure	0:13	Microstructure	0:13	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	0	32	theme	cell	42:45	arg1	suitability					55:65	cell culture suitability	42:65	cell culture suitability	42:65	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	4	33	theme	one-component	576:588	arg1	gels					590:593	Both one-component gels	571:593	Both one-component gels	571:593	Both one-component gels formed homogeneous networks, whereas hybrid gels were heterogeneous in terms of elasticity.					
30566463	6	34	theme	pure	898:901	arg1	gels					906:909	pure HA gels	898:909	pure HA gels	898:909	This was more pronounced in Coll and hybrid gels than in pure HA gels.					
30566463	4	35	from	heterogeneous	649:661	arg1	terms					666:670	terms	666:670	terms of elasticity	666:684	Both one-component gels formed homogeneous networks, whereas hybrid gels were heterogeneous in terms of elasticity.					
30566463	6	36	from	Coll	869:872	arg1	pronounced					855:864	pronounced	855:864	pronounced	855:864	This was more pronounced in Coll and hybrid gels than in pure HA gels.					
30566463	4	37	theme	hybrid	632:637	arg1	gels					639:642	hybrid gels	632:642	hybrid gels	632:642	Both one-component gels formed homogeneous networks, whereas hybrid gels were heterogeneous in terms of elasticity.					
30566463	1	38	theme	relevant	221:228	arg1	scale					237:241	a relevant length scale	219:241	a relevant length scale	219:241	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	6	39	from	gels	885:888	arg1	pronounced					855:864	pronounced	855:864	pronounced	855:864	This was more pronounced in Coll and hybrid gels than in pure HA gels.					
30566463	2	40	theme	bulk	358:361	arg1	measurements					363:374	bulk measurements	358:374	bulk measurements	358:374	We used multiple particle tracking microrheology to close the gap between elasticity determined from bulk measurements and elastic properties sensed by cells.					
30566463	0	41	theme	culture	47:53	arg1	Microstructure					0:13	Microstructure	0:13	Microstructure	0:13	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	0	41	theme	culture	47:53	arg1	suitability					55:65	cell culture suitability	42:65	cell culture suitability	42:65	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	5	42	theme	local	704:708	arg1	moduli					718:723	local elastic moduli	704:723	local elastic moduli	704:723	Most strikingly, local elastic moduli were significantly lower than bulk moduli presumably due to non-equilibrium chain conformations between crosslinks.					
30566463	1	43	from	scaffolds	206:214	arg1	scale					237:241	a relevant length scale	219:241	a relevant length scale	219:241	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	5	44	theme	elastic	710:716	arg1	moduli					718:723	local elastic moduli	704:723	local elastic moduli	704:723	Most strikingly, local elastic moduli were significantly lower than bulk moduli presumably due to non-equilibrium chain conformations between crosslinks.					
30566463	6	45	theme	hybrid	878:883	arg1	gels					885:888	hybrid gels	878:888	hybrid gels	878:888	This was more pronounced in Coll and hybrid gels than in pure HA gels.					
30566463	2	46	theme	multiple	265:272	arg1	tracking					283:290	multiple particle tracking	265:290	multiple particle tracking	265:290	We used multiple particle tracking microrheology to close the gap between elasticity determined from bulk measurements and elastic properties sensed by cells.					
30566463	1	47	theme	cell	131:134	arg1	substrates					144:153	cell culture substrates	131:153	cell culture substrates	131:153	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	1	48	theme	length	230:235	arg1	scale					237:241	a relevant length scale	219:241	a relevant length scale	219:241	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	1	49	from	scale	237:241	arg1	characterization					186:201	mechanical characterization	175:201	mechanical characterization of scaffolds on a relevant length scale	175:241	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	1	50	from	characterization	186:201	arg1	scale					237:241	a relevant length scale	219:241	a relevant length scale	219:241	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	1	51	theme	culture	136:142	arg1	substrates					144:153	cell culture substrates	131:153	cell culture substrates	131:153	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	3	52	theme	hyaluronic	510:519	arg1	HA					527:528	HA	527:528	HA	527:528	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	3	52	theme	hyaluronic	510:519	arg1	acid					521:524	hyaluronic acid	510:524	hyaluronic acid (HA)	510:529	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	0	53	theme	3D	70:71	arg1	scaffolds					92:100	3D hybrid HA/collagen scaffolds	70:100	3D hybrid HA/collagen scaffolds	70:100	Microstructure, local viscoelasticity and cell culture suitability of 3D hybrid HA/collagen scaffolds.					
30566463	1	54	theme	substrates	144:153	arg1	properties					117:126	mechanical properties	106:126	mechanical properties of cell culture substrates	106:153	As mechanical properties of cell culture substrates matter, methods for mechanical characterization of scaffolds on a relevant length scale are required.					
30566463	3	55	theme	cryogel	475:481	arg1	macroporous					444:454	macroporous	444:454	macroporous	444:454	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	3	55	theme	cryogel	475:481	arg1	scaffolds					483:491	three-dimensional cryogel scaffolds	457:491	three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll)	457:549	Structure and elasticity of macroporous, three-dimensional cryogel scaffolds from mixtures of hyaluronic acid (HA) and collagen (Coll) were characterized.					
30566463	5	56	theme	non-equilibrium	785:799	arg1	conformations					807:819	non-equilibrium chain conformations	785:819	non-equilibrium chain conformations	785:819	Most strikingly, local elastic moduli were significantly lower than bulk moduli presumably due to non-equilibrium chain conformations between crosslinks.					
30566463	2	57	theme	elastic	380:386	arg1	properties					388:397	elastic properties	380:397	elastic properties	380:397	We used multiple particle tracking microrheology to close the gap between elasticity determined from bulk measurements and elastic properties sensed by cells.					
30566463	7	58	theme	Local	912:916	arg1	moduli					926:931	Local elastic moduli	912:931	Local elastic moduli	912:931	Local elastic moduli were similar for all gels, irrespective of their different swelling ratio and bulk moduli.					
30566463	6	59	from	gels	906:909	arg1	pronounced					855:864	pronounced	855:864	pronounced	855:864	This was more pronounced in Coll and hybrid gels than in pure HA gels.					
29366892	5	0	theme	ratios	1051:1056	arg1	Linear					914:919	Linear	914:919	Linear	914:919	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	9	1	theme	Cationic	1550:1557	arg1	pectonanoliposomes					1559:1576	Cationic pectonanoliposomes	1550:1576	Cationic pectonanoliposomes	1550:1576	Cationic pectonanoliposomes were superior to plain systems regarding long-term stability.					
29366892	2	2	theme	methoxyl	409:416	arg1	pectin					418:423	low methoxyl pectin	405:423	low methoxyl pectin using an electrostatic deposition approach	405:466	Phloridzin-loaded nanoliposomes were fabricated using a heating-stirring-sonication method, and coated with low methoxyl pectin using an electrostatic deposition approach.					
29366892	6	3	theme	polynomial	1172:1181	arg1	models					1194:1199	Second-order polynomial regression models	1159:1199	Second-order polynomial regression models	1159:1199	Second-order polynomial regression models were generated for intensity-weighted particle size and zeta potential of the designed carriers.					
29366892	7	4	theme	contrast	1320:1327	arg1	images					1329:1334	Topographic and phase contrast images	1298:1334	images	1329:1334	Topographic and phase contrast images showed that pectonanoliposomes exhibited a range of different morphologies.					
29366892	4	5	used	used	804:807	arg2	methodology					788:798	Response surface methodology	771:798	Response surface methodology	771:798	Response surface methodology was used to optimize the composition of the pectonanoliposomes based on particle size and charge characteristics.					
29366892	5	6	theme	pectin/liposome	1035:1049	arg1	ratios					1051:1056	pectin/liposome ratios	1035:1056	pectin/liposome ratios	1035:1056	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	6	7	theme	regression	1183:1192	arg1	models					1194:1199	Second-order polynomial regression models	1159:1199	Second-order polynomial regression models	1159:1199	Second-order polynomial regression models were generated for intensity-weighted particle size and zeta potential of the designed carriers.					
29366892	5	8	theme	pectonanoliposomes	1139:1156	arg1	diameter					1105:1112	mean hydrodynamic diameter	1087:1112	mean hydrodynamic diameter	1087:1112	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	3	9	from	impact	600:605	arg1	stability					665:673	stability	665:673	stability	665:673	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	9	from	impact	600:605	arg1	morphology					650:659	morphology	650:659	morphology	650:659	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	9	from	impact	600:605	arg1	charge					642:647	charge	642:647	charge	642:647	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	9	from	impact	600:605	arg1	size					636:639	size	636:639	size	636:639	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	4	10	theme	charge	890:895	arg1	characteristics					897:911	particle size and charge characteristics	872:911	particle size and charge characteristics	872:911	Response surface methodology was used to optimize the composition of the pectonanoliposomes based on particle size and charge characteristics.					
29366892	5	11	theme	ammonium	984:991	arg1	propane/lecithin					993:1008	1,2-dioleoyl-3-trimethyl ammonium propane/lecithin	959:1008	1,2-dioleoyl-3-trimethyl ammonium propane/lecithin	959:1008	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	1	12	theme	colloidal	128:136	arg1	systems					147:153	Nanostructured colloidal delivery systems	113:153	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes)	113:216	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	1	12	theme	colloidal	128:136	arg1	carriers					236:243	carriers	236:243	carriers for a bioactive polyphenolic compound (phloridzin)	236:294	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	6	13	theme	zeta	1257:1260	arg1	potential					1262:1270	zeta potential	1257:1270	zeta potential	1257:1270	Second-order polynomial regression models were generated for intensity-weighted particle size and zeta potential of the designed carriers.					
29366892	3	14	theme	system	610:615	arg1	composition					617:627	system composition	610:627	system composition	610:627	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	15	theme	atomic	518:523	arg1	microscopy					531:540	atomic force microscopy	518:540	atomic force microscopy	518:540	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	16	theme	light	477:481	arg1	scattering					483:492	Dynamic light scattering	469:492	Dynamic light scattering	469:492	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	7	17	theme	phase	1314:1318	arg1	images					1329:1334	Topographic and phase contrast images	1298:1334	images	1329:1334	Topographic and phase contrast images showed that pectonanoliposomes exhibited a range of different morphologies.					
29366892	3	18	theme	composition	617:627	arg1	efficiencies					731:742	immobilization, adsorption and encapsulation efficiencies	686:742	the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes	596:768	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	18	theme	composition	617:627	arg1	impact					600:605	the impact	596:605	the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes	596:768	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	19	theme	force	525:529	arg1	microscopy					531:540	atomic force microscopy	518:540	atomic force microscopy	518:540	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	7	20	theme	morphologies	1398:1409	arg1	range					1379:1383	a range	1377:1383	a range of different morphologies	1377:1409	Topographic and phase contrast images showed that pectonanoliposomes exhibited a range of different morphologies.					
29366892	9	21	theme	plain	1595:1599	arg1	systems					1601:1607	plain systems	1595:1607	plain systems regarding long-term stability	1595:1637	Cationic pectonanoliposomes were superior to plain systems regarding long-term stability.					
29366892	6	22	theme	carriers	1288:1295	arg1	size					1248:1251	intensity-weighted particle size	1220:1251	intensity-weighted particle size	1220:1251	Second-order polynomial regression models were generated for intensity-weighted particle size and zeta potential of the designed carriers.					
29366892	6	22	theme	carriers	1288:1295	arg1	potential					1262:1270	zeta potential	1257:1270	zeta potential	1257:1270	Second-order polynomial regression models were generated for intensity-weighted particle size and zeta potential of the designed carriers.					
29366892	10	23	theme	polyphenolic	1721:1732	arg1	phloridizin					1758:1768	phloridizin	1758:1768	phloridizin	1758:1768	Our results suggest that pectonanoliposomes may be suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin, in functional food and pharmaceutical applications.					
29366892	10	23	theme	polyphenolic	1721:1732	arg1	nutraceuticals					1734:1747	polyphenolic nutraceuticals	1721:1747	polyphenolic nutraceuticals	1721:1747	Our results suggest that pectonanoliposomes may be suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin, in functional food and pharmaceutical applications.					
29366892	5	24	theme	surface	1121:1127	arg1	charge					1129:1134	surface charge	1121:1134	surface charge	1121:1134	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	2	25	theme	deposition	448:457	arg1	approach					459:466	an electrostatic deposition approach	431:466	an electrostatic deposition approach	431:466	Phloridzin-loaded nanoliposomes were fabricated using a heating-stirring-sonication method, and coated with low methoxyl pectin using an electrostatic deposition approach.					
29366892	6	26	theme	particle	1239:1246	arg1	size					1248:1251	intensity-weighted particle size	1220:1251	intensity-weighted particle size	1220:1251	Second-order polynomial regression models were generated for intensity-weighted particle size and zeta potential of the designed carriers.					
29366892	6	27	theme	Second-order	1159:1170	arg1	models					1194:1199	Second-order polynomial regression models	1159:1199	Second-order polynomial regression models	1159:1199	Second-order polynomial regression models were generated for intensity-weighted particle size and zeta potential of the designed carriers.					
29366892	7	28	theme	Topographic	1298:1308	arg1	images					1329:1334	Topographic and phase contrast images	1298:1334	images	1329:1334	Topographic and phase contrast images showed that pectonanoliposomes exhibited a range of different morphologies.					
29366892	10	29	theme	delivery	1700:1707	arg1	systems					1709:1715	suitable delivery systems	1691:1715	suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin,	1691:1769	Our results suggest that pectonanoliposomes may be suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin, in functional food and pharmaceutical applications.					
29366892	2	30	theme	electrostatic	434:446	arg1	approach					459:466	an electrostatic deposition approach	431:466	an electrostatic deposition approach	431:466	Phloridzin-loaded nanoliposomes were fabricated using a heating-stirring-sonication method, and coated with low methoxyl pectin using an electrostatic deposition approach.					
29366892	10	31	from	systems	1709:1715	arg1	applications					1809:1820	functional food and pharmaceutical applications	1774:1820	functional food and pharmaceutical applications	1774:1820	Our results suggest that pectonanoliposomes may be suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin, in functional food and pharmaceutical applications.					
29366892	6	32	theme	designed	1279:1286	arg1	carriers					1288:1295	the designed carriers	1275:1295	the designed carriers	1275:1295	Second-order polynomial regression models were generated for intensity-weighted particle size and zeta potential of the designed carriers.					
29366892	10	33	theme	pharmaceutical	1794:1807	arg1	applications					1809:1820	functional food and pharmaceutical applications	1774:1820	functional food and pharmaceutical applications	1774:1820	Our results suggest that pectonanoliposomes may be suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin, in functional food and pharmaceutical applications.					
29366892	5	34	theme	hydrodynamic	1092:1103	arg1	diameter					1105:1112	mean hydrodynamic diameter	1087:1112	mean hydrodynamic diameter	1087:1112	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	2	35	theme	heating-stirring-sonication	353:379	arg1	method					381:386	a heating-stirring-sonication method	351:386	a heating-stirring-sonication method	351:386	Phloridzin-loaded nanoliposomes were fabricated using a heating-stirring-sonication method, and coated with low methoxyl pectin using an electrostatic deposition approach.					
29366892	5	36	theme	interaction	936:946	arg1	effects					948:954	interaction effects	936:954	interaction effects	936:954	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	7	37	theme	different	1388:1396	arg1	morphologies					1398:1409	different morphologies	1388:1409	different morphologies	1388:1409	Topographic and phase contrast images showed that pectonanoliposomes exhibited a range of different morphologies.					
29366892	9	38	theme	long-term	1619:1627	arg1	stability					1629:1637	long-term stability	1619:1637	long-term stability	1619:1637	Cationic pectonanoliposomes were superior to plain systems regarding long-term stability.					
29366892	4	39	theme	size	881:884	arg1	characteristics					897:911	particle size and charge characteristics	872:911	particle size and charge characteristics	872:911	Response surface methodology was used to optimize the composition of the pectonanoliposomes based on particle size and charge characteristics.					
29366892	3	40	theme	encapsulation	717:729	arg1	efficiencies					731:742	immobilization, adsorption and encapsulation efficiencies	686:742	the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes	596:768	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	1	41	theme	Nanostructured	113:126	arg1	systems					147:153	Nanostructured colloidal delivery systems	113:153	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes)	113:216	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	1	41	theme	Nanostructured	113:126	arg1	carriers					236:243	carriers	236:243	carriers for a bioactive polyphenolic compound (phloridzin)	236:294	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	3	42	theme	UV-Visible	547:556	arg1	spectroscopy					558:569	UV-Visible spectroscopy	547:569	UV-Visible spectroscopy	547:569	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	0	43	theme	nanoliposomal	40:52	arg1	systems					63:69	pectin-coated nanoliposomal delivery systems	26:69	pectin-coated nanoliposomal delivery systems	26:69	Design and fabrication of pectin-coated nanoliposomal delivery systems for a bioactive polyphenolic: Phloridzin.					
29366892	10	44	theme	food	1785:1788	arg1	applications					1809:1820	functional food and pharmaceutical applications	1774:1820	functional food and pharmaceutical applications	1774:1820	Our results suggest that pectonanoliposomes may be suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin, in functional food and pharmaceutical applications.					
29366892	8	45	theme	storage	1531:1537	arg1	stability					1539:1547	physical storage stability	1522:1547	their immobilization and encapsulation efficiencies as well as physical storage stability	1459:1547	Coating the nanoliposomes with pectin improved their immobilization and encapsulation efficiencies as well as physical storage stability.					
29366892	5	46	theme	phloridzin/lecithin	1011:1029	arg1	Linear					914:919	Linear	914:919	Linear	914:919	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	5	47	dep	diameter	1105:1112	arg1	the					1083:1085	the	1083:1085	the	1083:1085	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	0	48	theme	pectin-coated	26:38	arg1	systems					63:69	pectin-coated nanoliposomal delivery systems	26:69	pectin-coated nanoliposomal delivery systems	26:69	Design and fabrication of pectin-coated nanoliposomal delivery systems for a bioactive polyphenolic: Phloridzin.					
29366892	10	49	theme	functional	1774:1783	arg1	applications					1809:1820	functional food and pharmaceutical applications	1774:1820	functional food and pharmaceutical applications	1774:1820	Our results suggest that pectonanoliposomes may be suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin, in functional food and pharmaceutical applications.					
29366892	3	50	theme	adsorption	702:711	arg1	efficiencies					731:742	immobilization, adsorption and encapsulation efficiencies	686:742	the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes	596:768	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	51	from	efficiencies	731:742	arg1	stability					665:673	stability	665:673	stability	665:673	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	51	from	efficiencies	731:742	arg1	morphology					650:659	morphology	650:659	morphology	650:659	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	51	from	efficiencies	731:742	arg1	charge					642:647	charge	642:647	charge	642:647	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	51	from	efficiencies	731:742	arg1	size					636:639	size	636:639	size	636:639	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	0	52	theme	systems	63:69	arg1	fabrication					11:21	fabrication	11:21	fabrication	11:21	Design and fabrication of pectin-coated nanoliposomal delivery systems for a bioactive polyphenolic: Phloridzin.					
29366892	0	52	theme	systems	63:69	arg1	Design					0:5	Design	0:5	Design	0:5	Design and fabrication of pectin-coated nanoliposomal delivery systems for a bioactive polyphenolic: Phloridzin.					
29366892	8	53	with	nanoliposomes	1424:1436	arg1	pectin					1443:1448	pectin	1443:1448	pectin	1443:1448	Coating the nanoliposomes with pectin improved their immobilization and encapsulation efficiencies as well as physical storage stability.					
29366892	2	54	theme	low	405:407	arg1	pectin					418:423	low methoxyl pectin	405:423	low methoxyl pectin using an electrostatic deposition approach	405:466	Phloridzin-loaded nanoliposomes were fabricated using a heating-stirring-sonication method, and coated with low methoxyl pectin using an electrostatic deposition approach.					
29366892	0	55	theme	delivery	54:61	arg1	systems					63:69	pectin-coated nanoliposomal delivery systems	26:69	pectin-coated nanoliposomal delivery systems	26:69	Design and fabrication of pectin-coated nanoliposomal delivery systems for a bioactive polyphenolic: Phloridzin.					
29366892	3	56	theme	pectonanoliposomes	751:768	arg1	efficiencies					731:742	immobilization, adsorption and encapsulation efficiencies	686:742	the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes	596:768	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	56	theme	pectonanoliposomes	751:768	arg1	impact					600:605	the impact	596:605	the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes	596:768	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	57	used	used	576:579	arg2	micro-electrophoresis					495:515	micro-electrophoresis	495:515	micro-electrophoresis	495:515	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	57	used	used	576:579	arg2	scattering					483:492	Dynamic light scattering	469:492	Dynamic light scattering	469:492	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	57	used	used	576:579	arg2	microscopy					531:540	atomic force microscopy	518:540	atomic force microscopy	518:540	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	3	57	used	used	576:579	arg2	spectroscopy					558:569	UV-Visible spectroscopy	547:569	UV-Visible spectroscopy	547:569	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	1	58	theme	delivery	138:145	arg1	systems					147:153	Nanostructured colloidal delivery systems	113:153	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes)	113:216	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	1	58	theme	delivery	138:145	arg1	carriers					236:243	carriers	236:243	carriers for a bioactive polyphenolic compound (phloridzin)	236:294	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	10	59	theme	suitable	1691:1698	arg1	systems					1709:1715	suitable delivery systems	1691:1715	suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin,	1691:1769	Our results suggest that pectonanoliposomes may be suitable delivery systems for polyphenolic nutraceuticals, such as phloridizin, in functional food and pharmaceutical applications.					
29366892	3	60	theme	immobilization	686:699	arg1	efficiencies					731:742	immobilization, adsorption and encapsulation efficiencies	686:742	the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes	596:768	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	2	61	theme	Phloridzin-loaded	297:313	arg1	nanoliposomes					315:327	Phloridzin-loaded nanoliposomes	297:327	Phloridzin-loaded nanoliposomes	297:327	Phloridzin-loaded nanoliposomes were fabricated using a heating-stirring-sonication method, and coated with low methoxyl pectin using an electrostatic deposition approach.					
29366892	6	62	theme	intensity-weighted	1220:1237	arg1	size					1248:1251	intensity-weighted particle size	1220:1251	intensity-weighted particle size	1220:1251	Second-order polynomial regression models were generated for intensity-weighted particle size and zeta potential of the designed carriers.					
29366892	5	63	theme	mean	1087:1090	arg1	diameter					1105:1112	mean hydrodynamic diameter	1087:1112	mean hydrodynamic diameter	1087:1112	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	4	64	theme	pectonanoliposomes	844:861	arg1	composition					825:835	the composition	821:835	the composition of the pectonanoliposomes based on particle size and charge characteristics	821:911	Response surface methodology was used to optimize the composition of the pectonanoliposomes based on particle size and charge characteristics.					
29366892	1	65	theme	bioactive	251:259	arg1	compound					274:281	a bioactive polyphenolic compound	249:281	a bioactive polyphenolic compound (phloridzin)	249:294	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	1	65	theme	bioactive	251:259	arg1	phloridzin					284:293	phloridzin	284:293	phloridzin	284:293	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	4	66	theme	particle	872:879	arg1	size					881:884	particle size	872:884	particle size	872:884	Response surface methodology was used to optimize the composition of the pectonanoliposomes based on particle size and charge characteristics.					
29366892	8	67	theme	Coating	1412:1418	arg1	nanoliposomes					1424:1436	the nanoliposomes	1420:1436	Coating the nanoliposomes with pectin	1412:1448	Coating the nanoliposomes with pectin improved their immobilization and encapsulation efficiencies as well as physical storage stability.					
29366892	8	68	theme	encapsulation	1484:1496	arg1	efficiencies					1498:1509	their immobilization and encapsulation efficiencies	1459:1509	efficiencies	1498:1509	Coating the nanoliposomes with pectin improved their immobilization and encapsulation efficiencies as well as physical storage stability.					
29366892	4	69	theme	Response	771:778	arg1	methodology					788:798	Response surface methodology	771:798	Response surface methodology	771:798	Response surface methodology was used to optimize the composition of the pectonanoliposomes based on particle size and charge characteristics.					
29366892	1	70	theme	polyphenolic	261:272	arg1	compound					274:281	a bioactive polyphenolic compound	249:281	a bioactive polyphenolic compound (phloridzin)	249:294	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	1	70	theme	polyphenolic	261:272	arg1	phloridzin					284:293	phloridzin	284:293	phloridzin	284:293	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	5	71	theme	1,2-dioleoyl-3-trimethyl	959:982	arg1	propane/lecithin					993:1008	1,2-dioleoyl-3-trimethyl ammonium propane/lecithin	959:1008	1,2-dioleoyl-3-trimethyl ammonium propane/lecithin	959:1008	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	8	72	theme	physical	1522:1529	arg1	stability					1539:1547	physical storage stability	1522:1547	their immobilization and encapsulation efficiencies as well as physical storage stability	1459:1547	Coating the nanoliposomes with pectin improved their immobilization and encapsulation efficiencies as well as physical storage stability.					
29366892	4	73	theme	surface	780:786	arg1	methodology					788:798	Response surface methodology	771:798	Response surface methodology	771:798	Response surface methodology was used to optimize the composition of the pectonanoliposomes based on particle size and charge characteristics.					
29366892	5	74	theme	propane/lecithin	993:1008	arg1	Linear					914:919	Linear	914:919	Linear	914:919	Linear, quadratic and interaction effects of 1,2-dioleoyl-3-trimethyl ammonium propane/lecithin, phloridzin/lecithin and pectin/liposome ratios significantly influenced the mean hydrodynamic diameter and/or surface charge of pectonanoliposomes.					
29366892	3	75	theme	Dynamic	469:475	arg1	scattering					483:492	Dynamic light scattering	469:492	Dynamic light scattering	469:492	Dynamic light scattering, micro-electrophoresis, atomic force microscopy, and UV-Visible spectroscopy were used to investigate the impact of system composition on the size, charge, morphology and stability as well as immobilization, adsorption and encapsulation efficiencies of the pectonanoliposomes.					
29366892	0	76	dep	Design	0:5	arg1	Phloridzin					101:110	Phloridzin	101:110	Design and fabrication of pectin-coated nanoliposomal delivery systems for a bioactive polyphenolic: Phloridzin.	0:111	Design and fabrication of pectin-coated nanoliposomal delivery systems for a bioactive polyphenolic: Phloridzin.					
29366892	1	77	theme	pectin-coated	169:181	arg1	pectonanoliposomes					198:215	pectonanoliposomes	198:215	pectonanoliposomes	198:215	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
29366892	1	77	theme	pectin-coated	169:181	arg1	nanoliposomes					183:195	pectin-coated nanoliposomes	169:195	pectin-coated nanoliposomes (pectonanoliposomes)	169:216	Nanostructured colloidal delivery systems comprising of pectin-coated nanoliposomes (pectonanoliposomes) were developed as carriers for a bioactive polyphenolic compound (phloridzin).					
30007626	0	0	theme	molecular	96:104	arg1	host					106:109	a multi-guest molecular host	82:109	a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells	82:179	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	3	1	with	DMAPA-Amp	650:658	arg1	PpIX					665:668	PpIX	665:668	PpIX	665:668	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	3	1	with	DMAPA-Amp	650:658	arg1	acid					680:683	folic acid	674:683	folic acid	674:683	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	3	2	theme	two-dimensional	744:758	arg1	NMR					801:803	two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR	744:803	two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR	744:803	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	3	3	theme	diffusion	760:768	arg1	NMR					801:803	two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR	744:803	two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR	744:803	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	0	4	theme	multi-guest	84:94	arg1	host					106:109	a multi-guest molecular host	82:109	a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells	82:179	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	5	5	theme	cytometry	1184:1192	arg1	analysis					1194:1201	flow cytometry analysis	1179:1201	flow cytometry analysis	1179:1201	The complex showed high phototoxicity against Panc-1 cells, and its folic-acid-mediated cancer-cell-targeting property was confirmed by laser confocal microscopy and flow cytometry analysis.					
30007626	5	6	theme	cancer-cell-targeting	1101:1121	arg1	property					1123:1130	its folic-acid-mediated cancer-cell-targeting property	1077:1130	its folic-acid-mediated cancer-cell-targeting property	1077:1130	The complex showed high phototoxicity against Panc-1 cells, and its folic-acid-mediated cancer-cell-targeting property was confirmed by laser confocal microscopy and flow cytometry analysis.					
30007626	1	7	theme	cancer	480:485	arg1	cells					496:500	human pancreatic cancer (Panc-1) cells	463:500	human pancreatic cancer (Panc-1) cells	463:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	6	8	dep	provide	1207:1213	arg1	facilitate					1320:1329	facilitate	1320:1329	could facilitate the design of multi-functional complexes in the drug delivery field	1314:1397	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	3	9	theme	folic	674:678	arg1	acid					680:683	folic acid	674:683	folic acid	674:683	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	4	10	theme	folic	964:968	arg1	acid					970:973	folic acid	964:973	folic acid	964:973	The results indicated that the DMAPA-Amp derivative could serve as a host for the encapsulation of two guests, PpIX and folic acid, through intermolecular interactions.					
30007626	4	10	theme	folic	964:968	arg1	guests					947:952	two guests	943:952	two guests	943:952	The results indicated that the DMAPA-Amp derivative could serve as a host for the encapsulation of two guests, PpIX and folic acid, through intermolecular interactions.					
30007626	1	11	theme	multi-guest	386:396	arg1	host					408:411	a multi-guest molecular host	384:411	a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells	384:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	1	11	theme	multi-guest	386:396	arg1	derivative					292:301	a hyperbranched cationic amylopectin derivative	255:301	a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp)	255:361	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	6	12	theme	multi-functional	1345:1360	arg1	complexes					1362:1370	multi-functional complexes	1345:1370	multi-functional complexes in the drug delivery field	1345:1397	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	6	13	theme	hyperbranched	1233:1245	arg1	complexes					1270:1278	hyperbranched cationic polymer-based complexes	1233:1278	hyperbranched cationic polymer-based complexes containing multiple guests	1233:1305	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	6	14	from	design	1335:1340	arg1	field					1393:1397	the drug delivery field	1375:1397	the drug delivery field	1375:1397	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	2	15	theme	folic	544:548	arg1	factor					598:603	a tumor-targeting factor	580:603	a tumor-targeting factor	580:603	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	2	15	theme	folic	544:548	arg1	IX					530:531	protoporphyrin IX	515:531	protoporphyrin IX (PpIX)	515:538	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	2	15	theme	folic	544:548	arg1	acid					550:553	folic acid	544:553	folic acid	544:553	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	1	16	theme	molecular	398:406	arg1	host					408:411	a multi-guest molecular host	384:411	a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells	384:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	1	16	theme	molecular	398:406	arg1	derivative					292:301	a hyperbranched cationic amylopectin derivative	255:301	a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp)	255:361	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	1	17	theme	Panc-1	488:493	arg1	cells					496:500	human pancreatic cancer (Panc-1) cells	463:500	human pancreatic cancer (Panc-1) cells	463:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	1	18	theme	3-	319:320	arg1	DMAPA-Amp					352:360	DMAPA-Amp	352:360	DMAPA-Amp	352:360	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	1	18	theme	3-	319:320	arg1	-1-propylamine					336:349	3-(dimethylamino)-1-propylamine	319:349	3-(dimethylamino)-1-propylamine (DMAPA-Amp)	319:361	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	3	19	theme	ordered	770:776	arg1	spectroscopy					778:789	ordered spectroscopy	770:789	two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR	744:803	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	3	19	theme	ordered	770:776	arg1	DOSY					795:798	2D DOSY	792:798	2D DOSY	792:798	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	4	20	theme	DMAPA-Amp	875:883	arg1	derivative					885:894	the DMAPA-Amp derivative	871:894	the DMAPA-Amp derivative	871:894	The results indicated that the DMAPA-Amp derivative could serve as a host for the encapsulation of two guests, PpIX and folic acid, through intermolecular interactions.					
30007626	4	20	theme	DMAPA-Amp	875:883	arg1	host					913:916	a host	911:916	a host for the encapsulation of two guests, PpIX and folic acid	911:973	The results indicated that the DMAPA-Amp derivative could serve as a host for the encapsulation of two guests, PpIX and folic acid, through intermolecular interactions.					
30007626	6	21	theme	delivery	1384:1391	arg1	field					1393:1397	the drug delivery field	1375:1397	the drug delivery field	1375:1397	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	0	22	theme	targeted	115:122	arg1	delivery					124:131	targeted delivery	115:131	targeted delivery of a photosensitizer to pancreatic cancer cells	115:179	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	5	23	theme	confocal	1155:1162	arg1	microscopy					1164:1173	laser confocal microscopy	1149:1173	laser confocal microscopy	1149:1173	The complex showed high phototoxicity against Panc-1 cells, and its folic-acid-mediated cancer-cell-targeting property was confirmed by laser confocal microscopy and flow cytometry analysis.					
30007626	5	24	theme	flow	1179:1182	arg1	analysis					1194:1201	flow cytometry analysis	1179:1201	flow cytometry analysis	1179:1201	The complex showed high phototoxicity against Panc-1 cells, and its folic-acid-mediated cancer-cell-targeting property was confirmed by laser confocal microscopy and flow cytometry analysis.					
30007626	2	25	theme	protoporphyrin	515:528	arg1	PpIX					534:537	PpIX	534:537	PpIX	534:537	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	2	25	theme	protoporphyrin	515:528	arg1	factor					598:603	a tumor-targeting factor	580:603	a tumor-targeting factor	580:603	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	2	25	theme	protoporphyrin	515:528	arg1	IX					530:531	protoporphyrin IX	515:531	protoporphyrin IX (PpIX)	515:538	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	2	25	theme	protoporphyrin	515:528	arg1	acid					550:553	folic acid	544:553	folic acid	544:553	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	5	26	theme	laser	1149:1153	arg1	microscopy					1164:1173	laser confocal microscopy	1149:1173	laser confocal microscopy	1149:1173	The complex showed high phototoxicity against Panc-1 cells, and its folic-acid-mediated cancer-cell-targeting property was confirmed by laser confocal microscopy and flow cytometry analysis.					
30007626	1	27	theme	pancreatic	194:203	arg1	phototoxicity					220:232	pancreatic tumor-targeted phototoxicity	194:232	pancreatic tumor-targeted phototoxicity of photosensitizers	194:252	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	0	28	theme	Spectroscopic	0:12	arg1	investigation					14:26	Spectroscopic investigation	0:26	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.	0:180	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	5	29	theme	folic-acid-mediated	1081:1099	arg1	property					1123:1130	its folic-acid-mediated cancer-cell-targeting property	1077:1130	its folic-acid-mediated cancer-cell-targeting property	1077:1130	The complex showed high phototoxicity against Panc-1 cells, and its folic-acid-mediated cancer-cell-targeting property was confirmed by laser confocal microscopy and flow cytometry analysis.					
30007626	1	30	theme	tumor-targeted	205:218	arg1	phototoxicity					220:232	pancreatic tumor-targeted phototoxicity	194:232	pancreatic tumor-targeted phototoxicity of photosensitizers	194:252	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	4	31	theme	guests	947:952	arg1	encapsulation					926:938	the encapsulation	922:938	the encapsulation of two guests, PpIX and folic acid	922:973	The results indicated that the DMAPA-Amp derivative could serve as a host for the encapsulation of two guests, PpIX and folic acid, through intermolecular interactions.					
30007626	6	32	theme	complexes	1362:1370	arg1	design					1335:1340	the design	1331:1340	the design of multi-functional complexes in the drug delivery field	1331:1397	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	3	33	theme	spectroscopy	778:789	arg1	NMR					801:803	two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR	744:803	two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR	744:803	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	1	34	theme	targeted	421:428	arg1	delivery					430:437	the targeted delivery	417:437	the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells	417:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	0	35	theme	cationic	47:54	arg1	derivative					68:77	a hyperbranched cationic amylopectin derivative	31:77	a hyperbranched cationic amylopectin derivative	31:77	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	6	36	contain	containing	1280:1289	arg1	complexes					1270:1278	hyperbranched cationic polymer-based complexes	1233:1278	hyperbranched cationic polymer-based complexes containing multiple guests	1233:1305	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	6	36	contain	containing	1280:1289	arg2	guests					1300:1305	multiple guests	1291:1305	multiple guests	1291:1305	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	6	37	theme	cationic	1247:1254	arg1	complexes					1270:1278	hyperbranched cationic polymer-based complexes	1233:1278	hyperbranched cationic polymer-based complexes containing multiple guests	1233:1305	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	1	38	theme	photosensitizers	237:252	arg1	phototoxicity					220:232	pancreatic tumor-targeted phototoxicity	194:232	pancreatic tumor-targeted phototoxicity of photosensitizers	194:252	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	0	39	theme	hyperbranched	33:45	arg1	derivative					68:77	a hyperbranched cationic amylopectin derivative	31:77	a hyperbranched cationic amylopectin derivative	31:77	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	0	40	theme	photosensitizer	138:152	arg1	delivery					124:131	targeted delivery	115:131	targeted delivery of a photosensitizer to pancreatic cancer cells	115:179	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	3	41	theme	complexation	624:635	arg1	mechanism					637:645	The complexation mechanism	620:645	The complexation mechanism of DMAPA-Amp with PpIX and folic acid	620:683	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	2	42	dep	photosensitizer	560:574	arg1	a					558:558	a	558:558	a	558:558	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	0	43	theme	derivative	68:77	arg1	investigation					14:26	Spectroscopic investigation	0:26	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.	0:180	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	0	44	theme	cancer	168:173	arg1	cells					175:179	pancreatic cancer cells	157:179	pancreatic cancer cells	157:179	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	5	45	theme	Panc-1	1059:1064	arg1	cells					1066:1070	Panc-1 cells	1059:1070	Panc-1 cells	1059:1070	The complex showed high phototoxicity against Panc-1 cells, and its folic-acid-mediated cancer-cell-targeting property was confirmed by laser confocal microscopy and flow cytometry analysis.					
30007626	6	46	theme	drug	1379:1382	arg1	field					1393:1397	the drug delivery field	1375:1397	the drug delivery field	1375:1397	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	0	47	theme	amylopectin	56:66	arg1	derivative					68:77	a hyperbranched cationic amylopectin derivative	31:77	a hyperbranched cationic amylopectin derivative	31:77	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	6	48	from	field	1393:1397	arg1	design					1335:1340	the design	1331:1340	the design of multi-functional complexes in the drug delivery field	1331:1397	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	0	49	theme	pancreatic	157:166	arg1	cells					175:179	pancreatic cancer cells	157:179	pancreatic cancer cells	157:179	Spectroscopic investigation of a hyperbranched cationic amylopectin derivative as a multi-guest molecular host for targeted delivery of a photosensitizer to pancreatic cancer cells.					
30007626	1	50	theme	photosensitizer	444:458	arg1	delivery					430:437	the targeted delivery	417:437	the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells	417:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	6	51	theme	multiple	1291:1298	arg1	guests					1300:1305	multiple guests	1291:1305	multiple guests	1291:1305	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	5	52	theme	high	1032:1035	arg1	phototoxicity					1037:1049	high phototoxicity	1032:1049	high phototoxicity against Panc-1 cells	1032:1070	The complex showed high phototoxicity against Panc-1 cells, and its folic-acid-mediated cancer-cell-targeting property was confirmed by laser confocal microscopy and flow cytometry analysis.					
30007626	2	53	theme	tumor-targeting	582:596	arg1	factor					598:603	a tumor-targeting factor	580:603	a tumor-targeting factor	580:603	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	2	53	theme	tumor-targeting	582:596	arg1	IX					530:531	protoporphyrin IX	515:531	protoporphyrin IX (PpIX)	515:538	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	2	53	theme	tumor-targeting	582:596	arg1	acid					550:553	folic acid	544:553	folic acid	544:553	We selected protoporphyrin IX (PpIX) and folic acid as a photosensitizer and a tumor-targeting factor, respectively.					
30007626	3	54	theme	NMR	709:711	arg1	spectroscopy					713:724	NMR spectroscopy	709:724	NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy	709:841	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	3	55	theme	DMAPA-Amp	650:658	arg1	mechanism					637:645	The complexation mechanism	620:645	The complexation mechanism of DMAPA-Amp with PpIX and folic acid	620:683	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	1	56	theme	human	463:467	arg1	cells					496:500	human pancreatic cancer (Panc-1) cells	463:500	human pancreatic cancer (Panc-1) cells	463:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	1	57	theme	hyperbranched	257:269	arg1	host					408:411	a multi-guest molecular host	384:411	a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells	384:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	1	57	theme	hyperbranched	257:269	arg1	derivative					292:301	a hyperbranched cationic amylopectin derivative	255:301	a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp)	255:361	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	4	58	theme	intermolecular	984:997	arg1	interactions					999:1010	intermolecular interactions	984:1010	intermolecular interactions	984:1010	The results indicated that the DMAPA-Amp derivative could serve as a host for the encapsulation of two guests, PpIX and folic acid, through intermolecular interactions.					
30007626	1	59	theme	pancreatic	469:478	arg1	cells					496:500	human pancreatic cancer (Panc-1) cells	463:500	human pancreatic cancer (Panc-1) cells	463:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	6	60	theme	polymer-based	1256:1268	arg1	complexes					1270:1278	hyperbranched cationic polymer-based complexes	1233:1278	hyperbranched cationic polymer-based complexes containing multiple guests	1233:1305	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	6	61	from	complexes	1362:1370	arg1	field					1393:1397	the drug delivery field	1375:1397	the drug delivery field	1375:1397	We provide a method to study hyperbranched cationic polymer-based complexes containing multiple guests, which could facilitate the design of multi-functional complexes in the drug delivery field.					
30007626	1	62	theme	cationic	271:278	arg1	host					408:411	a multi-guest molecular host	384:411	a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells	384:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	1	62	theme	cationic	271:278	arg1	derivative					292:301	a hyperbranched cationic amylopectin derivative	255:301	a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp)	255:361	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	3	63	theme	1H	736:737	arg1	NMR					739:741	1H NMR	736:741	1H NMR	736:741	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	3	64	theme	UV-vis	823:828	arg1	spectroscopy					830:841	UV-vis spectroscopy	823:841	UV-vis spectroscopy	823:841	The complexation mechanism of DMAPA-Amp with PpIX and folic acid was characterized using NMR spectroscopy including 1H NMR, two-dimensional diffusion ordered spectroscopy (2D DOSY) NMR, fluorescence and UV-vis spectroscopy.					
30007626	1	65	theme	amylopectin	280:290	arg1	host					408:411	a multi-guest molecular host	384:411	a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells	384:500	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
30007626	1	65	theme	amylopectin	280:290	arg1	derivative					292:301	a hyperbranched cationic amylopectin derivative	255:301	a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp)	255:361	To increase pancreatic tumor-targeted phototoxicity of photosensitizers, a hyperbranched cationic amylopectin derivative conjugated with 3-(dimethylamino)-1-propylamine (DMAPA-Amp) was invevstigated as a multi-guest molecular host for the targeted delivery of a photosensitizer to human pancreatic cancer (Panc-1) cells.					
29447960	4	0	theme	polydimethylsiloxane	930:949	arg1	substrates					958:967	polydimethylsiloxane (PDMS) substrates	930:967	polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels	930:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	3	1	contain	possessed	611:619	arg1	hydrogel					602:609	The resulting hydrogel	588:609	The resulting hydrogel	588:609	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	3	1	contain	possessed	611:619	arg2	structure					637:645	hierarchical NN structure	621:645	hierarchical NN structure	621:645	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	3	2	theme	comparable	738:747	arg1	elasticity					727:736	high elasticity	722:736	high elasticity comparable to soft tissues	722:763	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	2	3	theme	delicate	377:384	arg1	microstructures					386:400	these delicate microstructures	371:400	these delicate microstructures in nature	371:410	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	8	4	theme	alginate	1810:1817	arg1	counterparts					1819:1830	alginate counterparts	1810:1830	alginate counterparts	1810:1830	Different from most reported stretchable alginate-based hydrogels, the NN hydrogel shows higher compressive strength but retains comparable softness to alginate counterparts.					
29447960	1	5	theme	crucial	277:283	arg1	role					285:288	a crucial role	275:288	a crucial role	275:288	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	4	6	theme	PDMS	952:955	arg1	substrates					958:967	polydimethylsiloxane (PDMS) substrates	930:967	polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels	930:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	6	7	theme	hydrogel	1271:1278	arg1	bonding					1256:1262	Firm bonding	1251:1262	Firm bonding of the hydrogel on silicone substrates	1251:1301	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	8	8	theme	higher	1747:1752	arg1	strength					1766:1773	higher compressive strength	1747:1773	higher compressive strength	1747:1773	Different from most reported stretchable alginate-based hydrogels, the NN hydrogel shows higher compressive strength but retains comparable softness to alginate counterparts.					
29447960	4	9	theme	friction	893:900	arg1	coefficient					902:912	a surface friction coefficient	883:912	a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels	883:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	9	10	theme	chemical	1940:1947	arg1	modification					1949:1960	chemical modification	1940:1960	chemical modification	1940:1960	This work further demonstrated the good integration of the tough hydrogel with silicone substrates through chemical modification and micropillar structures.					
29447960	7	11	theme	HEMA	1546:1549	arg1	monomers					1551:1558	HEMA monomers	1546:1558	HEMA monomers in alginate hydrogels	1546:1580	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	4	12	theme	comparable	914:923	arg1	coefficient					902:912	a surface friction coefficient	883:912	a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels	883:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	6	13	theme	Firm	1251:1254	arg1	bonding					1256:1262	Firm bonding	1251:1262	Firm bonding of the hydrogel on silicone substrates	1251:1301	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	6	14	theme	chemical	1336:1343	arg1	modification					1345:1356	facile chemical modification	1329:1356	facile chemical modification	1329:1356	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	3	15	theme	resulting	592:600	arg1	hydrogel					602:609	The resulting hydrogel	588:609	The resulting hydrogel	588:609	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	1	16	theme	reticular	199:207	arg1	tissue					220:225	reticular connective tissue	199:225	reticular connective tissue	199:225	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	7	17	from	monomers	1551:1558	arg1	hydrogels					1572:1580	alginate hydrogels	1563:1580	alginate hydrogels	1563:1580	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	2	18	theme	alginate	568:575	arg1	hydrogels					577:585	alginate hydrogels	568:585	alginate hydrogels	568:585	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	3	19	theme	strengthening	781:793	arg1	effect					795:800	a mutual strengthening effect	772:800	a mutual strengthening effect between the two networks	772:825	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	5	20	theme	good	1136:1139	arg1	biocompatibility					1141:1156	good biocompatibility	1136:1156	good biocompatibility	1136:1156	Additional properties of this hydrogel include high hydrophilicity, good biocompatibility, tunable cell adhesion and bacterial resistance after incorporation of silver nanoparticles.					
29447960	6	21	theme	facile	1329:1334	arg1	modification					1345:1356	facile chemical modification	1329:1356	facile chemical modification	1329:1356	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	6	22	theme	versatile	1403:1411	arg1	use					1377:1379	the use	1373:1379	the use of this hydrogel	1373:1396	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	6	22	theme	versatile	1403:1411	arg1	material					1421:1428	a versatile coating material	1401:1428	a versatile coating material for biomedical applications	1401:1456	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	3	23	theme	NN	634:635	arg1	structure					637:645	hierarchical NN structure	621:645	hierarchical NN structure	621:645	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	1	24	theme	remarkable	235:244	arg1	strength					257:264	remarkable mechanical strength	235:264	remarkable mechanical strength	235:264	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	10	25	theme	coating	2149:2155	arg1	material					2157:2164	a versatile coating material	2137:2164	a versatile coating material for biomedical applications	2137:2192	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	10	25	theme	coating	2149:2155	arg1	promise					2126:2132	a great promise	2118:2132	a great promise	2118:2132	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	1	26	theme	hierarchical	137:148	arg1	structure					170:178	hierarchical nested-network (NN) structure	137:178	hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue	137:225	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	4	27	theme	other	1030:1034	arg1	hydrogels					1057:1065	other low surface friction hydrogels	1030:1065	other low surface friction hydrogels	1030:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	6	28	theme	silicone	1283:1290	arg1	substrates					1292:1301	silicone substrates	1283:1301	silicone substrates	1283:1301	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	4	29	with	comparable	914:923	arg1	substrates					958:967	polydimethylsiloxane (PDMS) substrates	930:967	polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels	930:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	4	30	theme	surface	1040:1046	arg1	hydrogels					1057:1065	other low surface friction hydrogels	1030:1065	other low surface friction hydrogels	1030:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	9	31	theme	good	1868:1871	arg1	integration					1873:1883	the good integration	1864:1883	the good integration of the tough hydrogel with silicone substrates	1864:1930	This work further demonstrated the good integration of the tough hydrogel with silicone substrates through chemical modification and micropillar structures.					
29447960	9	32	theme	tough	1892:1896	arg1	hydrogel					1898:1905	the tough hydrogel	1888:1905	the tough hydrogel	1888:1905	This work further demonstrated the good integration of the tough hydrogel with silicone substrates through chemical modification and micropillar structures.					
29447960	1	33	from	structure	170:178	arg1	tissue					220:225	reticular connective tissue	199:225	reticular connective tissue	199:225	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	1	33	from	structure	170:178	arg1	tissue					188:193	bone tissue	183:193	bone tissue	183:193	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	4	34	theme	K-Y	1015:1017	arg1	Jelly					1019:1023	K-Y Jelly	1015:1023	K-Y Jelly	1015:1023	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	4	34	theme	K-Y	1015:1017	arg1	lubricant					1004:1012	lubricant	1004:1012	lubricant	1004:1012	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	5	35	theme	cell	1167:1170	arg1	adhesion					1172:1179	tunable cell adhesion	1159:1179	tunable cell adhesion	1159:1179	Additional properties of this hydrogel include high hydrophilicity, good biocompatibility, tunable cell adhesion and bacterial resistance after incorporation of silver nanoparticles.					
29447960	9	36	theme	silicone	1912:1919	arg1	substrates					1921:1930	silicone substrates	1912:1930	silicone substrates	1912:1930	This work further demonstrated the good integration of the tough hydrogel with silicone substrates through chemical modification and micropillar structures.					
29447960	6	37	from	bonding	1256:1262	arg1	substrates					1292:1301	silicone substrates	1283:1301	silicone substrates	1283:1301	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	6	38	theme	biomedical	1434:1443	arg1	applications					1445:1456	biomedical applications	1434:1456	biomedical applications	1434:1456	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	9	39	theme	micropillar	1966:1976	arg1	structures					1978:1987	micropillar structures	1966:1987	micropillar structures	1966:1987	This work further demonstrated the good integration of the tough hydrogel with silicone substrates through chemical modification and micropillar structures.					
29447960	2	40	theme	pHEMA	526:530	arg1	network					533:539	a poly(2-hydroxyethyl methacrylate) (pHEMA) network	489:539	a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels	489:585	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	1	41	theme	composite	108:116	arg1	structure					170:178	hierarchical nested-network (NN) structure	137:178	hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue	137:225	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	1	41	theme	composite	108:116	arg1	formations					118:127	Well-organized composite formations	93:127	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue	93:225	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	2	42	theme	hydrogel	453:460	arg1	scaffold					426:433	an analogous scaffold	413:433	an analogous scaffold of double network hydrogel	413:460	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	7	43	theme	network	1644:1650	arg1	structure					1611:1619	a well-organized structure	1594:1619	a well-organized structure of hierarchical nested network (NN)	1594:1655	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	2	44	theme	2-hydroxyethyl	496:509	arg1	network					533:539	a poly(2-hydroxyethyl methacrylate) (pHEMA) network	489:539	a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels	489:585	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	10	45	theme	surface	2017:2023	arg1	friction					2025:2032	surface friction	2017:2032	surface friction	2017:2032	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	2	46	theme	double	438:443	arg1	hydrogel					453:460	double network hydrogel	438:460	double network hydrogel	438:460	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	5	47	theme	silver	1229:1234	arg1	nanoparticles					1236:1248	silver nanoparticles	1229:1248	silver nanoparticles	1229:1248	Additional properties of this hydrogel include high hydrophilicity, good biocompatibility, tunable cell adhesion and bacterial resistance after incorporation of silver nanoparticles.					
29447960	7	48	theme	hierarchical	1624:1635	arg1	NN					1653:1654	NN	1653:1654	NN	1653:1654	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	7	48	theme	hierarchical	1624:1635	arg1	network					1644:1650	hierarchical nested network	1624:1650	hierarchical nested network (NN)	1624:1655	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	2	49	theme	poly	491:494	arg1	network					533:539	a poly(2-hydroxyethyl methacrylate) (pHEMA) network	489:539	a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels	489:585	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	3	50	theme	mechanical	681:690	arg1	strength					692:699	mechanical strength	681:699	mechanical strength	681:699	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	0	51	theme	coating	52:58	arg1	material					60:67	a coating material	50:67	a coating material for biomedical devices	50:90	Mechanically enhanced nested-network hydrogels as a coating material for biomedical devices.					
29447960	1	52	theme	living	341:346	arg1	organisms					348:356	living organisms	341:356	living organisms	341:356	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	5	53	theme	hydrogel	1098:1105	arg1	properties					1079:1088	Additional properties	1068:1088	Additional properties of this hydrogel	1068:1105	Additional properties of this hydrogel include high hydrophilicity, good biocompatibility, tunable cell adhesion and bacterial resistance after incorporation of silver nanoparticles.					
29447960	8	54	theme	reported	1678:1685	arg1	hydrogels					1714:1722	most reported stretchable alginate-based hydrogels	1673:1722	most reported stretchable alginate-based hydrogels	1673:1722	Different from most reported stretchable alginate-based hydrogels, the NN hydrogel shows higher compressive strength but retains comparable softness to alginate counterparts.					
29447960	8	55	theme	compressive	1754:1764	arg1	strength					1766:1773	higher compressive strength	1747:1773	higher compressive strength	1747:1773	Different from most reported stretchable alginate-based hydrogels, the NN hydrogel shows higher compressive strength but retains comparable softness to alginate counterparts.					
29447960	1	56	theme	mechanical	246:255	arg1	strength					257:264	remarkable mechanical strength	235:264	remarkable mechanical strength	235:264	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	8	57	theme	alginate-based	1699:1712	arg1	hydrogels					1714:1722	most reported stretchable alginate-based hydrogels	1673:1722	most reported stretchable alginate-based hydrogels	1673:1722	Different from most reported stretchable alginate-based hydrogels, the NN hydrogel shows higher compressive strength but retains comparable softness to alginate counterparts.					
29447960	5	58	theme	Additional	1068:1077	arg1	properties					1079:1088	Additional properties	1068:1088	Additional properties of this hydrogel	1068:1105	Additional properties of this hydrogel include high hydrophilicity, good biocompatibility, tunable cell adhesion and bacterial resistance after incorporation of silver nanoparticles.					
29447960	8	59	theme	comparable	1787:1796	arg1	softness					1798:1805	comparable softness	1787:1805	comparable softness to alginate counterparts	1787:1830	Different from most reported stretchable alginate-based hydrogels, the NN hydrogel shows higher compressive strength but retains comparable softness to alginate counterparts.					
29447960	7	60	theme	alginate	1563:1570	arg1	hydrogels					1572:1580	alginate hydrogels	1563:1580	alginate hydrogels	1563:1580	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	8	61	theme	NN	1729:1730	arg1	hydrogel					1732:1739	the NN hydrogel	1725:1739	the NN hydrogel	1725:1739	Different from most reported stretchable alginate-based hydrogels, the NN hydrogel shows higher compressive strength but retains comparable softness to alginate counterparts.					
29447960	3	62	theme	mutual	774:779	arg1	effect					795:800	a mutual strengthening effect	772:800	a mutual strengthening effect between the two networks	772:825	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	1	63	theme	bone	183:186	arg1	tissue					188:193	bone tissue	183:193	bone tissue	183:193	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	4	64	theme	surface	885:891	arg1	coefficient					902:912	a surface friction coefficient	883:912	a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels	883:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	2	65	from	microstructures	386:400	arg1	nature					405:410	nature	405:410	nature	405:410	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	7	66	theme	tough	1515:1519	arg1	hydrogel					1521:1528	a tough hydrogel	1513:1528	a tough hydrogel	1513:1528	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	4	67	theme	low	1036:1038	arg1	hydrogels					1057:1065	other low surface friction hydrogels	1030:1065	other low surface friction hydrogels	1030:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	10	68	theme	bacterial	2056:2064	arg1	resistance					2066:2075	bacterial resistance	2056:2075	bacterial resistance	2056:2075	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	1	69	theme	connective	209:218	arg1	tissue					220:225	reticular connective tissue	199:225	reticular connective tissue	199:225	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	10	70	theme	great	2120:2124	arg1	material					2157:2164	a versatile coating material	2137:2164	a versatile coating material for biomedical applications	2137:2192	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	10	70	theme	great	2120:2124	arg1	promise					2126:2132	a great promise	2118:2132	a great promise	2118:2132	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	6	71	theme	coating	1413:1419	arg1	use					1377:1379	the use	1373:1379	the use of this hydrogel	1373:1396	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	6	71	theme	coating	1413:1419	arg1	material					1421:1428	a versatile coating material	1401:1428	a versatile coating material for biomedical applications	1401:1456	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	3	72	theme	hierarchical	621:632	arg1	structure					637:645	hierarchical NN structure	621:645	hierarchical NN structure	621:645	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	3	73	dep	showed	651:656	arg1	maintained					711:720	maintained	711:720	maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks	711:825	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	3	73	dep	showed	651:656	arg1	improved					672:679	improved	672:679	improved mechanical strength	672:699	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	2	74	theme	porous	548:553	arg1	structure					555:563	the porous structure	544:563	the porous structure of alginate hydrogels	544:585	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	9	75	theme	hydrogel	1898:1905	arg1	integration					1873:1883	the good integration	1864:1883	the good integration of the tough hydrogel with silicone substrates	1864:1930	This work further demonstrated the good integration of the tough hydrogel with silicone substrates through chemical modification and micropillar structures.					
29447960	5	76	theme	tunable	1159:1165	arg1	adhesion					1172:1179	tunable cell adhesion	1159:1179	tunable cell adhesion	1159:1179	Additional properties of this hydrogel include high hydrophilicity, good biocompatibility, tunable cell adhesion and bacterial resistance after incorporation of silver nanoparticles.					
29447960	2	77	from	network	533:539	arg1	structure					555:563	the porous structure	544:563	the porous structure of alginate hydrogels	544:585	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	7	78	theme	SIGNIFICANCE	1472:1483	arg1	STATEMENT					1459:1467	STATEMENT	1459:1467	STATEMENT OF SIGNIFICANCE	1459:1483	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	10	79	theme	versatile	2139:2147	arg1	material					2157:2164	a versatile coating material	2137:2164	a versatile coating material for biomedical applications	2137:2192	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	10	79	theme	versatile	2139:2147	arg1	promise					2126:2132	a great promise	2118:2132	a great promise	2118:2132	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	6	80	theme	hydrogel	1389:1396	arg1	material					1421:1428	a versatile coating material	1401:1428	a versatile coating material for biomedical applications	1401:1456	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	6	80	theme	hydrogel	1389:1396	arg1	use					1377:1379	the use	1373:1379	the use of this hydrogel	1373:1396	Firm bonding of the hydrogel on silicone substrates could be achieved through facile chemical modification, thus enabling the use of this hydrogel as a versatile coating material for biomedical applications.					
29447960	5	81	theme	high	1115:1118	arg1	hydrophilicity					1120:1133	high hydrophilicity	1115:1133	high hydrophilicity	1115:1133	Additional properties of this hydrogel include high hydrophilicity, good biocompatibility, tunable cell adhesion and bacterial resistance after incorporation of silver nanoparticles.					
29447960	10	82	theme	Other	1990:1994	arg1	biocompatibility					2035:2050	biocompatibility	2035:2050	biocompatibility	2035:2050	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	10	82	theme	Other	1990:1994	arg1	properties					1996:2005	Other properties	1990:2005	Other properties including surface friction, biocompatibility and bacterial resistance	1990:2075	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	10	82	theme	Other	1990:1994	arg1	friction					2025:2032	surface friction	2017:2032	surface friction	2017:2032	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	10	82	theme	Other	1990:1994	arg1	resistance					2066:2075	bacterial resistance	2056:2075	bacterial resistance	2056:2075	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	1	83	theme	nested-network	150:163	arg1	structure					170:178	hierarchical nested-network (NN) structure	137:178	hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue	137:225	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	2	84	theme	hydrogels	577:585	arg1	structure					555:563	the porous structure	544:563	the porous structure of alginate hydrogels	544:585	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	0	85	theme	biomedical	73:82	arg1	devices					84:90	biomedical devices	73:90	biomedical devices	73:90	Mechanically enhanced nested-network hydrogels as a coating material for biomedical devices.					
29447960	9	86	with	integration	1873:1883	arg1	substrates					1921:1930	silicone substrates	1912:1930	silicone substrates	1912:1930	This work further demonstrated the good integration of the tough hydrogel with silicone substrates through chemical modification and micropillar structures.					
29447960	10	87	theme	biomedical	2170:2179	arg1	applications					2181:2192	biomedical applications	2170:2192	biomedical applications	2170:2192	Other properties including surface friction, biocompatibility and bacterial resistance were investigated and the hydrogel shows a great promise as a versatile coating material for biomedical applications.					
29447960	2	88	theme	methacrylate	511:522	arg1	network					533:539	a poly(2-hydroxyethyl methacrylate) (pHEMA) network	489:539	a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels	489:585	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	2	89	theme	analogous	416:424	arg1	scaffold					426:433	an analogous scaffold	413:433	an analogous scaffold of double network hydrogel	413:460	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	0	90	theme	enhanced	13:20	arg1	nested-network					22:35	Mechanically enhanced nested-network	0:35	Mechanically enhanced nested-network	0:35	Mechanically enhanced nested-network hydrogels as a coating material for biomedical devices.					
29447960	5	91	theme	bacterial	1185:1193	arg1	resistance					1195:1204	bacterial resistance	1185:1204	bacterial resistance	1185:1204	Additional properties of this hydrogel include high hydrophilicity, good biocompatibility, tunable cell adhesion and bacterial resistance after incorporation of silver nanoparticles.					
29447960	1	92	theme	Well-organized	93:106	arg1	structure					170:178	hierarchical nested-network (NN) structure	137:178	hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue	137:225	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	1	92	theme	Well-organized	93:106	arg1	formations					118:127	Well-organized composite formations	93:127	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue	93:225	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	8	93	from	hydrogels	1714:1722	arg1	Different					1658:1666	Different	1658:1666	Different	1658:1666	Different from most reported stretchable alginate-based hydrogels, the NN hydrogel shows higher compressive strength but retains comparable softness to alginate counterparts.					
29447960	1	94	theme	physical	303:310	arg1	functions					327:335	physical and biological functions	303:335	physical and biological functions for living organisms	303:356	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	2	95	theme	network	445:451	arg1	hydrogel					453:460	double network hydrogel	438:460	double network hydrogel	438:460	Inspired by these delicate microstructures in nature, an analogous scaffold of double network hydrogel was fabricated by creating a poly(2-hydroxyethyl methacrylate) (pHEMA) network in the porous structure of alginate hydrogels.					
29447960	1	96	theme	biological	316:325	arg1	functions					327:335	physical and biological functions	303:335	physical and biological functions for living organisms	303:356	Well-organized composite formations such as hierarchical nested-network (NN) structure in bone tissue and reticular connective tissue present remarkable mechanical strength and play a crucial role in achieving physical and biological functions for living organisms.					
29447960	4	97	theme	friction	1048:1055	arg1	hydrogels					1057:1065	other low surface friction hydrogels	1030:1065	other low surface friction hydrogels	1030:1065	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	5	98	theme	nanoparticles	1236:1248	arg1	incorporation					1212:1224	incorporation	1212:1224	incorporation of silver nanoparticles	1212:1248	Additional properties of this hydrogel include high hydrophilicity, good biocompatibility, tunable cell adhesion and bacterial resistance after incorporation of silver nanoparticles.					
29447960	7	99	theme	nested	1637:1642	arg1	NN					1653:1654	NN	1653:1654	NN	1653:1654	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	7	99	theme	nested	1637:1642	arg1	network					1644:1650	hierarchical nested network	1624:1650	hierarchical nested network (NN)	1624:1655	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	8	100	theme	stretchable	1687:1697	arg1	hydrogels					1714:1722	most reported stretchable alginate-based hydrogels	1673:1722	most reported stretchable alginate-based hydrogels	1673:1722	Different from most reported stretchable alginate-based hydrogels, the NN hydrogel shows higher compressive strength but retains comparable softness to alginate counterparts.					
29447960	3	101	theme	high	722:725	arg1	elasticity					727:736	high elasticity	722:736	high elasticity comparable to soft tissues	722:763	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
29447960	4	102	theme	tough	832:836	arg1	hydrogel					838:845	The tough hydrogel	828:845	The tough hydrogel	828:845	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	4	102	theme	tough	832:836	arg1	self-lubricated					855:869	self-lubricated	855:869	self-lubricated	855:869	The tough hydrogel is also self-lubricated, exhibiting a surface friction coefficient comparable with polydimethylsiloxane (PDMS) substrates lubricated by a commercial aqueous lubricant (K-Y Jelly) and other low surface friction hydrogels.					
29447960	7	103	theme	well-organized	1596:1609	arg1	structure					1611:1619	a well-organized structure	1594:1619	a well-organized structure of hierarchical nested network (NN)	1594:1655	STATEMENT OF SIGNIFICANCE In this study, we developed a tough hydrogel by crosslinking HEMA monomers in alginate hydrogels and forming a well-organized structure of hierarchical nested network (NN).					
29447960	3	104	theme	soft	752:755	arg1	tissues					757:763	soft tissues	752:763	soft tissues	752:763	The resulting hydrogel possessed hierarchical NN structure and showed significantly improved mechanical strength but still maintained high elasticity comparable to soft tissues due to a mutual strengthening effect between the two networks.					
30383847	4	0	theme	sulfated	518:525	arg1	vizantin					527:534	100 μM sulfated vizantin	511:534	100 μM sulfated vizantin	511:534	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	6	1	theme	motility	1303:1310	arg1	vizantin					1255:1262	sulfated vizantin	1246:1262	sulfated vizantin	1246:1262	These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.					
30383847	6	1	theme	motility	1303:1310	arg1	inhibitor					1280:1288	an effective inhibitor	1267:1288	an effective inhibitor of flagellar motility in P. aeruginosa	1267:1327	These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.					
30383847	5	2	theme	mRNA	931:934	arg1	expression					936:945	mRNA expression	931:945	mRNA expression of several flagella-related genes	931:979	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	3	theme	P.	817:818	arg1	PAO1					831:834	P. aeruginosa PAO1	817:834	P. aeruginosa PAO1	817:834	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	4	4	theme	P.	547:548	arg1	PAO1					561:564	P. aeruginosa PAO1	547:564	P. aeruginosa PAO1	547:564	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	6	5	theme	flagellar	1293:1301	arg1	motility					1303:1310	flagellar motility	1293:1310	flagellar motility	1293:1310	These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.					
30383847	5	6	dep	complex	1064:1070	arg1	motA					1079:1082	motA	1079:1082	motA	1079:1082	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	6	dep	complex	1064:1070	arg1	motB					1089:1092	motB	1089:1092	motB	1089:1092	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	6	dep	complex	1064:1070	arg1	fliC					1073:1076	fliC	1073:1076	fliC	1073:1076	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	7	theme	sulfated	762:769	arg1	vizantin					771:778	sulfated vizantin	762:778	sulfated vizantin	762:778	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	3	8	theme	P.	415:416	arg1	motility					439:446	P. aeruginosa flagellar motility	415:446	P. aeruginosa flagellar motility	415:446	In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity.					
30383847	4	9	theme	bacterial	691:699	arg1	activity					710:717	bacterial protease activity	691:717	bacterial protease activity	691:717	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	4	10	theme	flagellar	630:638	arg1	motility					640:647	flagellar motility	630:647	flagellar motility	630:647	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	0	11	theme	Pseudomonas	91:101	arg1	PAO1					114:117	Pseudomonas aeruginosa PAO1	91:117	Pseudomonas aeruginosa PAO1	91:117	Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1.					
30383847	5	12	from	effects	853:859	arg1	expression					936:945	mRNA expression	931:945	mRNA expression of several flagella-related genes	931:979	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	12	from	effects	853:859	arg1	composition					889:899	the composition	885:899	the composition of the flagellar filament	885:925	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	3	13	dep	P.	415:416	arg1	aeruginosa					418:427	aeruginosa	418:427	aeruginosa	418:427	In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity.					
30383847	2	14	theme	virulence	306:314	arg1	factor					316:321	an important virulence factor	293:321	an important virulence factor for P. aeruginosa infection	293:349	Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection.					
30383847	2	14	theme	virulence	306:314	arg1	motility					281:288	flagellar motility	271:288	flagellar motility	271:288	Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection.					
30383847	6	15	from	inhibitor	1280:1288	arg1	aeruginosa					1318:1327	P. aeruginosa	1315:1327	P. aeruginosa	1315:1327	These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.					
30383847	5	16	dep	genes	1188:1192	arg1	genes					1188:1192	the chemotaxis-related genes cheR1, cheW, and cheZ	1165:1214	the chemotaxis-related genes cheR1, cheW, and cheZ	1165:1214	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	16	dep	genes	1188:1192	arg1	cheW					1201:1204	cheW	1201:1204	cheW	1201:1204	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	16	dep	genes	1188:1192	arg1	cheZ					1211:1214	cheZ	1211:1214	cheZ	1211:1214	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	16	dep	genes	1188:1192	arg1	cheR1					1194:1198	cheR1	1194:1198	cheR1	1194:1198	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	6	17	theme	effective	1270:1278	arg1	vizantin					1255:1262	sulfated vizantin	1246:1262	sulfated vizantin	1246:1262	These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.					
30383847	6	17	theme	effective	1270:1278	arg1	inhibitor					1280:1288	an effective inhibitor	1267:1288	an effective inhibitor of flagellar motility in P. aeruginosa	1267:1327	These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.					
30383847	2	18	theme	important	296:304	arg1	factor					316:321	an important virulence factor	293:321	an important virulence factor for P. aeruginosa infection	293:349	Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection.					
30383847	2	18	theme	important	296:304	arg1	motility					281:288	flagellar motility	271:288	flagellar motility	271:288	Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection.					
30383847	1	19	theme	severe	184:189	arg1	pneumonia					211:219	pneumonia	211:219	pneumonia	211:219	Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia.					
30383847	1	19	theme	severe	184:189	arg1	infections					191:200	severe infections	184:200	severe infections	184:200	Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia.					
30383847	1	19	theme	severe	184:189	arg1	bacteremia					225:234	bacteremia	225:234	bacteremia	225:234	Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia.					
30383847	0	20	theme	PAO1	114:117	arg1	motility					79:86	the flagella motility	66:86	the flagella motility of Pseudomonas aeruginosa PAO1	66:117	Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1.					
30383847	4	21	theme	μM	515:516	arg1	vizantin					527:534	100 μM sulfated vizantin	511:534	100 μM sulfated vizantin	511:534	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	0	22	dep	Pseudomonas	91:101	arg1	aeruginosa					103:112	aeruginosa	103:112	aeruginosa	103:112	Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1.					
30383847	3	23	theme	direct	466:471	arg1	activity					487:494	direct antimicrobial activity	466:494	direct antimicrobial activity	466:494	In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity.					
30383847	4	24	theme	100	511:513	arg1	μM					515:516	μM	515:516	μM	515:516	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	5	25	theme	flagellar	795:803	arg1	motility					805:812	the flagellar motility	791:812	the flagellar motility of P. aeruginosa PAO1	791:834	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	26	from	composition	1035:1045	arg1	PAO1					1112:1115	P. aeruginosa PAO1	1098:1115	P. aeruginosa PAO1	1098:1115	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	0	27	theme	Sulfated	0:7	arg1	vizantin					9:16	Sulfated vizantin	0:16	Sulfated vizantin	0:16	Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1.					
30383847	2	28	theme	aeruginosa	330:339	arg1	infection					341:349	P. aeruginosa infection	327:349	P. aeruginosa infection	327:349	Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection.					
30383847	0	29	theme	dependent	53:61	arg1	penetration					41:51	mucin layer penetration	29:51	mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1	29:117	Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1.					
30383847	4	30	theme	protease	701:708	arg1	activity					710:717	bacterial protease activity	691:717	bacterial protease activity	691:717	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	3	31	theme	sulfated	389:396	arg1	vizantin					398:405	sulfated vizantin	389:405	sulfated vizantin	389:405	In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity.					
30383847	2	32	theme	P.	327:328	arg1	infection					341:349	P. aeruginosa infection	327:349	P. aeruginosa infection	327:349	Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection.					
30383847	6	33	theme	sulfated	1246:1253	arg1	vizantin					1255:1262	sulfated vizantin	1246:1262	sulfated vizantin	1246:1262	These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.					
30383847	6	33	theme	sulfated	1246:1253	arg1	inhibitor					1280:1288	an effective inhibitor	1267:1288	an effective inhibitor of flagellar motility in P. aeruginosa	1267:1327	These results indicated that sulfated vizantin is an effective inhibitor of flagellar motility in P. aeruginosa.					
30383847	5	34	theme	sulfated	864:871	arg1	vizantin					873:880	sulfated vizantin	864:880	sulfated vizantin	864:880	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	4	35	theme	mucin	605:609	arg1	layer					611:615	an artificial mucin layer	591:615	an artificial mucin layer	591:615	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	5	36	dep	P.	817:818	arg1	aeruginosa					820:829	aeruginosa	820:829	aeruginosa	820:829	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	37	theme	sulfated	995:1002	arg1	vizantin					1004:1011	sulfated vizantin	995:1011	sulfated vizantin	995:1011	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	38	theme	vizantin	873:880	arg1	effects					853:859	the effects	849:859	the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes	849:979	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	4	39	theme	artificial	594:603	arg1	layer					611:615	an artificial mucin layer	591:615	an artificial mucin layer	591:615	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	5	40	theme	flagellar	1054:1062	arg1	complex					1064:1070	the flagellar complex	1050:1070	the flagellar complex (fliC, motA, and motB)	1050:1093	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	41	theme	flagellar	908:916	arg1	filament					918:925	the flagellar filament	904:925	the flagellar filament	904:925	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	3	42	theme	antimicrobial	473:485	arg1	activity					487:494	direct antimicrobial activity	466:494	direct antimicrobial activity	466:494	In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity.					
30383847	0	43	theme	layer	35:39	arg1	penetration					41:51	mucin layer penetration	29:51	mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1	29:117	Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1.					
30383847	4	44	dep	P.	547:548	arg1	aeruginosa					550:559	aeruginosa	550:559	aeruginosa	550:559	We found that 100 μM sulfated vizantin suppressed P. aeruginosa PAO1 from penetrating through an artificial mucin layer by affecting flagellar motility, although it did not influence growth nor bacterial protease activity.					
30383847	5	45	theme	PAO1	831:834	arg1	motility					805:812	the flagellar motility	791:812	the flagellar motility of P. aeruginosa PAO1	791:834	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	0	46	theme	mucin	29:33	arg1	penetration					41:51	mucin layer penetration	29:51	mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1	29:117	Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1.					
30383847	3	47	theme	activity	487:494	arg1	absence					455:461	the absence	451:461	the absence of direct antimicrobial activity	451:494	In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity.					
30383847	5	48	theme	filament	918:925	arg1	expression					936:945	mRNA expression	931:945	mRNA expression of several flagella-related genes	931:979	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	48	theme	filament	918:925	arg1	composition					889:899	the composition	885:899	the composition of the flagellar filament	885:925	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	49	theme	P.	1098:1099	arg1	PAO1					1112:1115	P. aeruginosa PAO1	1098:1115	P. aeruginosa PAO1	1098:1115	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	2	50	theme	Several	237:243	arg1	studies					245:251	Several studies	237:251	Several studies	237:251	Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection.					
30383847	5	51	theme	mRNA	1146:1149	arg1	expression					1151:1160	mRNA expression	1146:1160	mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ	1146:1214	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	3	52	theme	flagellar	429:437	arg1	motility					439:446	P. aeruginosa flagellar motility	415:446	P. aeruginosa flagellar motility	415:446	In this study, we determined whether sulfated vizantin affects P. aeruginosa flagellar motility in the absence of direct antimicrobial activity.					
30383847	5	53	theme	chemotaxis-related	1169:1186	arg1	genes					1188:1192	the chemotaxis-related genes cheR1, cheW, and cheZ	1165:1214	the chemotaxis-related genes cheR1, cheW, and cheZ	1165:1214	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	53	theme	chemotaxis-related	1169:1186	arg1	cheW					1201:1204	cheW	1201:1204	cheW	1201:1204	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	53	theme	chemotaxis-related	1169:1186	arg1	cheZ					1211:1214	cheZ	1211:1214	cheZ	1211:1214	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	53	theme	chemotaxis-related	1169:1186	arg1	cheR1					1194:1198	cheR1	1194:1198	cheR1	1194:1198	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	54	theme	several	950:956	arg1	genes					975:979	several flagella-related genes	950:979	several flagella-related genes	950:979	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	55	theme	genes	1188:1192	arg1	expression					1151:1160	mRNA expression	1146:1160	mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ	1146:1214	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	56	theme	complex	1064:1070	arg1	composition					1035:1045	the composition	1031:1045	the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1	1031:1115	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	57	theme	flagella-related	958:973	arg1	genes					975:979	several flagella-related genes	950:979	several flagella-related genes	950:979	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	58	theme	genes	975:979	arg1	expression					936:945	mRNA expression	931:945	mRNA expression of several flagella-related genes	931:979	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	58	theme	genes	975:979	arg1	composition					889:899	the composition	885:899	the composition of the flagellar filament	885:925	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	5	59	dep	P.	1098:1099	arg1	aeruginosa					1101:1110	aeruginosa	1101:1110	aeruginosa	1101:1110	To further clarify the mechanism in which sulfated vizantin suppresses the flagellar motility of P. aeruginosa PAO1, we examined the effects of sulfated vizantin on the composition of the flagellar filament and mRNA expression of several flagella-related genes, finding that sulfated vizantin did not influence the composition of the flagellar complex (fliC, motA, and motB) in P. aeruginosa PAO1, but significantly decreased mRNA expression of the chemotaxis-related genes cheR1, cheW, and cheZ.					
30383847	0	60	theme	flagella	70:77	arg1	motility					79:86	the flagella motility	66:86	the flagella motility of Pseudomonas aeruginosa PAO1	66:117	Sulfated vizantin suppresses mucin layer penetration dependent on the flagella motility of Pseudomonas aeruginosa PAO1.					
30383847	2	61	theme	flagellar	271:279	arg1	factor					316:321	an important virulence factor	293:321	an important virulence factor for P. aeruginosa infection	293:349	Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection.					
30383847	2	61	theme	flagellar	271:279	arg1	motility					281:288	flagellar motility	271:288	flagellar motility	271:288	Several studies demonstrated that flagellar motility is an important virulence factor for P. aeruginosa infection.					
30383847	1	62	theme	opportunistic	149:161	arg1	pathogen					163:170	an opportunistic pathogen	146:170	an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia	146:234	Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia.					
30383847	1	62	theme	opportunistic	149:161	arg1	aeruginosa					132:141	Pseudomonas aeruginosa	120:141	Pseudomonas aeruginosa	120:141	Pseudomonas aeruginosa is an opportunistic pathogen that causes severe infections, such as pneumonia and bacteremia.					
30960915	5	0	theme	weight	737:742	arg1	faster					776:781	faster	776:781	faster	776:781	Moreover, the weight loss rate of the composites was faster at 180⁻200 °C than that of CaAlg, after which the rate was less comparatively, suggesting the better thermal stability of the composite.					
30960915	5	0	theme	weight	737:742	arg1	rate					749:752	the weight loss rate	733:752	the weight loss rate of the composites	733:770	Moreover, the weight loss rate of the composites was faster at 180⁻200 °C than that of CaAlg, after which the rate was less comparatively, suggesting the better thermal stability of the composite.					
30960915	1	1	theme	functional	158:167	arg1	material					169:176	biodegradable and eco-friendly functional material	127:176	biodegradable and eco-friendly functional material	127:176	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	4	2	theme	Further	559:565	arg1	study					567:571	Further study	559:571	Further study	559:571	Further study showed that rGO increased the carbon formation rate by 4.1%, indicating that the thermal stability was improved by the promotion of carbon formation.					
30960915	6	3	theme	molecule	1069:1076	arg1	chain					1078:1082	the glycan molecule chain	1058:1082	the glycan molecule chain	1058:1082	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	4	4	theme	carbon	705:710	arg1	formation					712:720	carbon formation	705:720	carbon formation	705:720	Further study showed that rGO increased the carbon formation rate by 4.1%, indicating that the thermal stability was improved by the promotion of carbon formation.					
30960915	3	5	theme	thermal	531:537	arg1	stability					539:547	the thermal stability	527:547	the thermal stability of CaAlg	527:556	The results showed that the introduction of rGO can efficiently improve the thermal stability of CaAlg.					
30960915	4	6	theme	formation	610:618	arg1	rate					620:623	the carbon formation rate	599:623	the carbon formation rate	599:623	Further study showed that rGO increased the carbon formation rate by 4.1%, indicating that the thermal stability was improved by the promotion of carbon formation.					
30960915	5	7	theme	composite	909:917	arg1	stability					892:900	the better thermal stability	873:900	the better thermal stability of the composite	873:917	Moreover, the weight loss rate of the composites was faster at 180⁻200 °C than that of CaAlg, after which the rate was less comparatively, suggesting the better thermal stability of the composite.					
30960915	1	8	theme	material	169:176	arg1	kind					119:122	a kind	117:122	a kind of biodegradable and eco-friendly functional material	117:176	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	8	theme	material	169:176	arg1	alginate					97:104	Calcium alginate	89:104	Calcium alginate (CaAlg)	89:112	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	5	9	theme	loss	744:747	arg1	faster					776:781	faster	776:781	faster	776:781	Moreover, the weight loss rate of the composites was faster at 180⁻200 °C than that of CaAlg, after which the rate was less comparatively, suggesting the better thermal stability of the composite.					
30960915	5	9	theme	loss	744:747	arg1	rate					749:752	the weight loss rate	733:752	the weight loss rate of the composites	733:770	Moreover, the weight loss rate of the composites was faster at 180⁻200 °C than that of CaAlg, after which the rate was less comparatively, suggesting the better thermal stability of the composite.					
30960915	1	10	theme	Calcium	89:95	arg1	kind					119:122	a kind	117:122	a kind of biodegradable and eco-friendly functional material	117:176	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	10	theme	Calcium	89:95	arg1	CaAlg					107:111	CaAlg	107:111	CaAlg	107:111	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	10	theme	Calcium	89:95	arg1	alginate					97:104	Calcium alginate	89:104	Calcium alginate (CaAlg)	89:112	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	5	11	theme	composites	761:770	arg1	faster					776:781	faster	776:781	faster	776:781	Moreover, the weight loss rate of the composites was faster at 180⁻200 °C than that of CaAlg, after which the rate was less comparatively, suggesting the better thermal stability of the composite.					
30960915	5	11	theme	composites	761:770	arg1	rate					749:752	the weight loss rate	733:752	the weight loss rate of the composites	733:770	Moreover, the weight loss rate of the composites was faster at 180⁻200 °C than that of CaAlg, after which the rate was less comparatively, suggesting the better thermal stability of the composite.					
30960915	3	12	theme	CaAlg	552:556	arg1	stability					539:547	the thermal stability	527:547	the thermal stability of CaAlg	527:556	The results showed that the introduction of rGO can efficiently improve the thermal stability of CaAlg.					
30960915	2	13	theme	thermal	422:428	arg1	properties					430:439	their morphological and thermal properties	398:439	their morphological and thermal properties	398:439	In this paper, the CaAlg/rGO composites were prepared by a sol-gel method and their morphological and thermal properties were studied.					
30960915	1	14	theme	new	259:261	arg1	composites					218:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	14	theme	new	259:261	arg1	materials					309:317	new textile, heat-generating, and flame-retardant materials	259:317	new textile, heat-generating, and flame-retardant materials	259:317	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	5	15	theme	better	877:882	arg1	stability					892:900	the better thermal stability	873:900	the better thermal stability of the composite	873:917	Moreover, the weight loss rate of the composites was faster at 180⁻200 °C than that of CaAlg, after which the rate was less comparatively, suggesting the better thermal stability of the composite.					
30960915	1	16	theme	CaAlg/reduced	183:195	arg1	composites					218:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	16	theme	CaAlg/reduced	183:195	arg1	materials					309:317	new textile, heat-generating, and flame-retardant materials	259:317	new textile, heat-generating, and flame-retardant materials	259:317	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	17	theme	textile	263:269	arg1	composites					218:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	17	theme	textile	263:269	arg1	materials					309:317	new textile, heat-generating, and flame-retardant materials	259:317	new textile, heat-generating, and flame-retardant materials	259:317	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	5	18	theme	thermal	884:890	arg1	stability					892:900	the better thermal stability	873:900	the better thermal stability of the composite	873:917	Moreover, the weight loss rate of the composites was faster at 180⁻200 °C than that of CaAlg, after which the rate was less comparatively, suggesting the better thermal stability of the composite.					
30960915	1	19	theme	graphene	197:204	arg1	oxide					206:210	graphene oxide	197:210	CaAlg/reduced graphene oxide (rGO) composites	183:227	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	19	theme	graphene	197:204	arg1	rGO					213:215	rGO	213:215	rGO	213:215	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	2	20	theme	morphological	404:416	arg1	properties					430:439	their morphological and thermal properties	398:439	their morphological and thermal properties	398:439	In this paper, the CaAlg/rGO composites were prepared by a sol-gel method and their morphological and thermal properties were studied.					
30960915	4	21	theme	formation	712:720	arg1	promotion					692:700	the promotion	688:700	the promotion of carbon formation	688:720	Further study showed that rGO increased the carbon formation rate by 4.1%, indicating that the thermal stability was improved by the promotion of carbon formation.					
30960915	1	22	theme	oxide	206:210	arg1	composites					218:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	22	theme	oxide	206:210	arg1	materials					309:317	new textile, heat-generating, and flame-retardant materials	259:317	new textile, heat-generating, and flame-retardant materials	259:317	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	0	23	theme	Calcium	37:43	arg1	Morphology					0:9	Morphology	0:9	Morphology	0:9	Morphology and Thermal Properties of Calcium Alginate/Reduced Graphene Oxide Composites.					
30960915	0	23	theme	Calcium	37:43	arg1	Properties					23:32	Thermal Properties	15:32	Thermal Properties	15:32	Morphology and Thermal Properties of Calcium Alginate/Reduced Graphene Oxide Composites.					
30960915	1	24	theme	heat-generating	272:286	arg1	composites					218:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	24	theme	heat-generating	272:286	arg1	materials					309:317	new textile, heat-generating, and flame-retardant materials	259:317	new textile, heat-generating, and flame-retardant materials	259:317	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	6	25	theme	rGO	976:978	arg1	efficiency					962:971	the high heat transfer efficiency	939:971	the high heat transfer efficiency of rGO	939:978	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	4	26	theme	thermal	654:660	arg1	stability					662:670	the thermal stability	650:670	the thermal stability	650:670	Further study showed that rGO increased the carbon formation rate by 4.1%, indicating that the thermal stability was improved by the promotion of carbon formation.					
30960915	6	27	theme	heat	948:951	arg1	efficiency					962:971	the high heat transfer efficiency	939:971	the high heat transfer efficiency of rGO	939:978	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	2	28	theme	sol-gel	379:385	arg1	method					387:392	a sol-gel method	377:392	a sol-gel method	377:392	In this paper, the CaAlg/rGO composites were prepared by a sol-gel method and their morphological and thermal properties were studied.					
30960915	0	29	theme	Graphene	62:69	arg1	Composites					77:86	Graphene Oxide Composites	62:86	Graphene Oxide Composites	62:86	Morphology and Thermal Properties of Calcium Alginate/Reduced Graphene Oxide Composites.					
30960915	7	30	theme	cracking	1153:1160	arg1	mechanism					1162:1170	The thermal cracking mechanism	1141:1170	The thermal cracking mechanism of the composites	1141:1188	The thermal cracking mechanism of the composites is proposed based on the experimental data.					
30960915	6	31	theme	thermal	1033:1039	arg1	decomposition					1041:1053	the thermal decomposition	1029:1053	the thermal decomposition of the glycan molecule chain	1029:1082	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	6	32	theme	glycan	1062:1067	arg1	chain					1078:1082	the glycan molecule chain	1058:1082	the glycan molecule chain	1058:1082	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	1	33	theme	flame-retardant	293:307	arg1	composites					218:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	CaAlg/reduced graphene oxide (rGO) composites	183:227	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	1	33	theme	flame-retardant	293:307	arg1	materials					309:317	new textile, heat-generating, and flame-retardant materials	259:317	new textile, heat-generating, and flame-retardant materials	259:317	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	7	34	theme	composites	1179:1188	arg1	mechanism					1162:1170	The thermal cracking mechanism	1141:1170	The thermal cracking mechanism of the composites	1141:1188	The thermal cracking mechanism of the composites is proposed based on the experimental data.					
30960915	1	35	theme	biodegradable	127:139	arg1	material					169:176	biodegradable and eco-friendly functional material	127:176	biodegradable and eco-friendly functional material	127:176	Calcium alginate (CaAlg) is a kind of biodegradable and eco-friendly functional material, and CaAlg/reduced graphene oxide (rGO) composites are expected to be applied as new textile, heat-generating, and flame-retardant materials.					
30960915	6	36	theme	chain	1078:1082	arg1	decomposition					1041:1053	the thermal decomposition	1029:1053	the thermal decomposition of the glycan molecule chain	1029:1082	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	6	37	theme	short	1093:1097	arg1	time					1099:1102	a short time	1091:1102	a short time	1091:1102	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	6	38	theme	carbon	1123:1128	arg1	formation					1130:1138	carbon formation	1123:1138	carbon formation	1123:1138	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	6	39	theme	decomposition	1041:1053	arg1	temperature					1014:1024	the temperature	1010:1024	the temperature of the thermal decomposition of the glycan molecule chain	1010:1082	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	6	40	theme	transfer	953:960	arg1	efficiency					962:971	the high heat transfer efficiency	939:971	the high heat transfer efficiency of rGO	939:978	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
30960915	7	41	theme	thermal	1145:1151	arg1	mechanism					1162:1170	The thermal cracking mechanism	1141:1170	The thermal cracking mechanism of the composites	1141:1188	The thermal cracking mechanism of the composites is proposed based on the experimental data.					
30960915	7	42	theme	experimental	1215:1226	arg1	data					1228:1231	the experimental data	1211:1231	the experimental data	1211:1231	The thermal cracking mechanism of the composites is proposed based on the experimental data.					
30960915	3	43	theme	rGO	499:501	arg1	introduction					483:494	the introduction	479:494	the introduction of rGO	479:501	The results showed that the introduction of rGO can efficiently improve the thermal stability of CaAlg.					
30960915	4	44	theme	carbon	603:608	arg1	rate					620:623	the carbon formation rate	599:623	the carbon formation rate	599:623	Further study showed that rGO increased the carbon formation rate by 4.1%, indicating that the thermal stability was improved by the promotion of carbon formation.					
30960915	0	45	theme	Oxide	71:75	arg1	Composites					77:86	Graphene Oxide Composites	62:86	Graphene Oxide Composites	62:86	Morphology and Thermal Properties of Calcium Alginate/Reduced Graphene Oxide Composites.					
30960915	0	46	theme	Thermal	15:21	arg1	Properties					23:32	Thermal Properties	15:32	Thermal Properties	15:32	Morphology and Thermal Properties of Calcium Alginate/Reduced Graphene Oxide Composites.					
30960915	2	47	theme	CaAlg/rGO	339:347	arg1	composites					349:358	the CaAlg/rGO composites	335:358	the CaAlg/rGO composites	335:358	In this paper, the CaAlg/rGO composites were prepared by a sol-gel method and their morphological and thermal properties were studied.					
30960915	6	48	theme	high	943:946	arg1	efficiency					962:971	the high heat transfer efficiency	939:971	the high heat transfer efficiency of rGO	939:978	This maybe because the high heat transfer efficiency of rGO allowed the material to reach the temperature of the thermal decomposition of the glycan molecule chain within a short time, and then promoted carbon formation.					
31368554	0	0	theme	varieties	75:83	arg1	indices					56:62	glycemic indices	47:62	glycemic indices	47:62	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	0	0	theme	varieties	75:83	arg1	L.					123:124	Zea mays L.	114:124	Zea mays L.	114:124	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	0	0	theme	varieties	75:83	arg1	composition					7:17	Starch composition	0:17	Starch composition	0:17	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	0	0	theme	varieties	75:83	arg1	potential					32:40	antioxidant potential	20:40	antioxidant potential	20:40	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	2	1	theme	starch	396:401	arg1	composition					403:413	starch composition	396:413	starch composition	396:413	In this study, 17 wheat and 9 maize varieties were tested for starch composition, glycemic index, radical scavenging activities, and lipid profile.					
31368554	9	2	theme	important	1437:1445	arg1	role					1447:1450	an important role	1434:1450	an important role	1434:1450	Diet and nutrition play an important role in the development and control of diabetes.					
31368554	1	3	theme	Zea	207:209	arg1	L					217:217	Zea maize L	207:217	Zea maize L. (maize)	207:226	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	3	theme	Zea	207:209	arg1	maize					221:225	maize	221:225	maize	221:225	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	0	4	theme	various	67:73	arg1	Triticum					88:95	Triticum	88:95	Triticum	88:95	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	0	4	theme	various	67:73	arg1	varieties					75:83	various varieties	67:83	various varieties of Triticum aesitivum L.	67:108	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	4	5	from	56.45 ± 1.95	829:840	arg1	white					852:856	white	852:856	white	852:856	Glycemic index of wheat varieties varied between 62.83 ± 2.19 and 70.70 ± 2.82 in Hashim10 and Insaf, respectively, while for maize it ranged between 56.45 ± 1.95 in Sarhad white and 70.76 ± 4.40 in CHT1W.					
31368554	10	6	theme	grown	1609:1613	arg1	genotypes					1615:1623	locally grown genotypes	1601:1623	locally grown genotypes	1601:1623	In this research, we attempted to find out the best wheat and maize varieties for diabetic patients from locally grown genotypes.					
31368554	0	7	from	Pakistan	139:146	arg1	available					126:134	available	126:134	available	126:134	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	0	8	theme	Triticum	88:95	arg1	Triticum					88:95	Triticum	88:95	Triticum	88:95	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	0	8	theme	Triticum	88:95	arg1	varieties					75:83	various varieties	67:83	various varieties of Triticum aesitivum L.	67:108	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	5	9	theme	glycemic	953:960	arg1	index					962:966	glycemic index	953:966	glycemic index	953:966	In most of the varieties, a linear correlation was observed between glycemic index and amylose contents.					
31368554	4	10	theme	wheat	697:701	arg1	varieties					703:711	wheat varieties	697:711	wheat varieties	697:711	Glycemic index of wheat varieties varied between 62.83 ± 2.19 and 70.70 ± 2.82 in Hashim10 and Insaf, respectively, while for maize it ranged between 56.45 ± 1.95 in Sarhad white and 70.76 ± 4.40 in CHT1W.					
31368554	7	11	theme	diabetic	1304:1311	arg1	patients					1313:1320	diabetic patients	1304:1320	diabetic patients	1304:1320	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	1	12	theme	L	217:217	arg1	maize					221:225	maize	221:225	maize	221:225	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	12	theme	L	217:217	arg1	L					186:186	Triticum aesitivum L	167:186	Triticum aesitivum L. (Bread wheat)	167:201	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	12	theme	L	217:217	arg1	L					217:217	Zea maize L	207:217	Zea maize L. (maize)	207:226	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	12	theme	L	217:217	arg1	varieties					154:162	Many varieties	149:162	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize)	149:226	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	12	theme	L	217:217	arg1	wheat					196:200	Bread wheat	190:200	Bread wheat	190:200	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	3	13	theme	various	656:662	arg1	samples					670:676	various maize samples	656:676	various maize samples	656:676	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	7	14	dep	APPLICATIONS	1181:1192	arg1	APPLICATIONS					1181:1192	PRACTICAL APPLICATIONS	1171:1192	PRACTICAL APPLICATIONS: Wheat and maize	1171:1209	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	7	14	dep	APPLICATIONS	1181:1192	arg1	maize					1205:1209	maize	1205:1209	maize	1205:1209	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	7	14	dep	APPLICATIONS	1181:1192	arg1	Wheat					1195:1199	Wheat	1195:1199	Wheat	1195:1199	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	0	15	from	available	126:134	arg1	Pakistan					139:146	Pakistan	139:146	Pakistan	139:146	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	3	16	theme	maize	664:668	arg1	samples					670:676	various maize samples	656:676	various maize samples	656:676	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	3	17	contain	have	533:536	arg2	activity					594:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	3	17	contain	have	533:536	arg1	KPK15					511:515	KPK15	511:515	KPK15	511:515	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	0	18	dep	Triticum	88:95	arg1	L.					107:108	Triticum aesitivum L.	88:108	Triticum aesitivum L.	88:108	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	1	19	theme	Many	149:152	arg1	maize					221:225	maize	221:225	maize	221:225	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	19	theme	Many	149:152	arg1	L					186:186	Triticum aesitivum L	167:186	Triticum aesitivum L. (Bread wheat)	167:201	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	19	theme	Many	149:152	arg1	L					217:217	Zea maize L	207:217	Zea maize L. (maize)	207:226	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	19	theme	Many	149:152	arg1	varieties					154:162	Many varieties	149:162	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize)	149:226	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	19	theme	Many	149:152	arg1	wheat					196:200	Bread wheat	190:200	Bread wheat	190:200	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	2	20	theme	maize	364:368	arg1	varieties					370:378	17 wheat and 9 maize varieties	349:378	varieties	370:378	In this study, 17 wheat and 9 maize varieties were tested for starch composition, glycemic index, radical scavenging activities, and lipid profile.					
31368554	1	21	theme	normal	292:297	arg1	individuals					299:309	normal individuals	292:309	normal individuals	292:309	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	10	22	from	genotypes	1615:1623	arg1	maize					1558:1562	maize	1558:1562	maize	1558:1562	In this research, we attempted to find out the best wheat and maize varieties for diabetic patients from locally grown genotypes.					
31368554	10	22	from	genotypes	1615:1623	arg1	wheat					1548:1552	wheat	1548:1552	wheat	1548:1552	In this research, we attempted to find out the best wheat and maize varieties for diabetic patients from locally grown genotypes.					
31368554	4	23	theme	varieties	703:711	arg1	index					688:692	Glycemic index	679:692	Glycemic index of wheat varieties	679:711	Glycemic index of wheat varieties varied between 62.83 ± 2.19 and 70.70 ± 2.82 in Hashim10 and Insaf, respectively, while for maize it ranged between 56.45 ± 1.95 in Sarhad white and 70.76 ± 4.40 in CHT1W.					
31368554	7	24	theme	cereal	1236:1241	arg1	APPLICATIONS					1181:1192	PRACTICAL APPLICATIONS	1171:1192	PRACTICAL APPLICATIONS: Wheat and maize	1171:1209	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	7	24	theme	cereal	1236:1241	arg1	crops					1243:1247	the most widely used cereal crops	1215:1247	the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients	1215:1320	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	3	25	theme	radical	575:581	arg1	activity					594:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	6	26	theme	diabetic	1152:1159	arg1	patients					1161:1168	diabetic patients	1152:1168	diabetic patients	1152:1168	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	6	27	theme	white	1073:1077	arg1	Sarhad					1066:1071	Sarhad white	1066:1077	Sarhad white	1066:1077	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	6	27	theme	white	1073:1077	arg1	best					1086:1089	best	1086:1089	best	1086:1089	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	0	28	theme	Starch	0:5	arg1	composition					7:17	Starch composition	0:17	Starch composition	0:17	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	1	29	theme	Triticum	167:174	arg1	L					186:186	Triticum aesitivum L	167:186	Triticum aesitivum L. (Bread wheat)	167:201	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	29	theme	Triticum	167:174	arg1	wheat					196:200	Bread wheat	190:200	Bread wheat	190:200	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	0	30	theme	antioxidant	20:30	arg1	potential					32:40	antioxidant potential	20:40	antioxidant potential	20:40	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	10	31	dep	wheat	1548:1552	arg1	varieties					1564:1572	varieties	1564:1572	varieties	1564:1572	In this research, we attempted to find out the best wheat and maize varieties for diabetic patients from locally grown genotypes.					
31368554	10	31	dep	wheat	1548:1552	arg1	the					1539:1541	the	1539:1541	the	1539:1541	In this research, we attempted to find out the best wheat and maize varieties for diabetic patients from locally grown genotypes.					
31368554	0	32	theme	Zea	114:116	arg1	L.					123:124	Zea mays L.	114:124	Zea mays L.	114:124	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	1	33	theme	maize	211:215	arg1	L					217:217	Zea maize L	207:217	Zea maize L. (maize)	207:226	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	33	theme	maize	211:215	arg1	maize					221:225	maize	221:225	maize	221:225	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	34	theme	aesitivum	176:184	arg1	L					186:186	Triticum aesitivum L	167:186	Triticum aesitivum L. (Bread wheat)	167:201	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	34	theme	aesitivum	176:184	arg1	wheat					196:200	Bread wheat	190:200	Bread wheat	190:200	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	3	35	theme	2,2-diphenyl-1picrylhydrazyl	546:573	arg1	activity					594:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	4	36	theme	Glycemic	679:686	arg1	index					688:692	Glycemic index	679:692	Glycemic index of wheat varieties	679:711	Glycemic index of wheat varieties varied between 62.83 ± 2.19 and 70.70 ± 2.82 in Hashim10 and Insaf, respectively, while for maize it ranged between 56.45 ± 1.95 in Sarhad white and 70.76 ± 4.40 in CHT1W.					
31368554	1	37	theme	L	186:186	arg1	maize					221:225	maize	221:225	maize	221:225	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	37	theme	L	186:186	arg1	L					186:186	Triticum aesitivum L	167:186	Triticum aesitivum L. (Bread wheat)	167:201	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	37	theme	L	186:186	arg1	L					217:217	Zea maize L	207:217	Zea maize L. (maize)	207:226	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	37	theme	L	186:186	arg1	varieties					154:162	Many varieties	149:162	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize)	149:226	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	37	theme	L	186:186	arg1	wheat					196:200	Bread wheat	190:200	Bread wheat	190:200	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	38	theme	diabetic	315:322	arg1	patients					324:331	diabetic patients	315:331	diabetic patients	315:331	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	7	39	theme	normal	1281:1286	arg1	individuals					1288:1298	normal individuals	1281:1298	normal individuals	1281:1298	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	7	40	dep	individuals	1288:1298	arg1	both					1273:1276	both	1273:1276	both	1273:1276	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	7	41	theme	used	1231:1234	arg1	APPLICATIONS					1181:1192	PRACTICAL APPLICATIONS	1171:1192	PRACTICAL APPLICATIONS: Wheat and maize	1171:1209	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	7	41	theme	used	1231:1234	arg1	crops					1243:1247	the most widely used cereal crops	1215:1247	the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients	1215:1320	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	0	42	theme	mays	118:121	arg1	L.					123:124	Zea mays L.	114:124	Zea mays L.	114:124	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	6	43	theme	normal	1129:1134	arg1	individuals					1136:1146	normal individuals	1129:1146	normal individuals	1129:1146	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	2	44	theme	glycemic	416:423	arg1	index					425:429	glycemic index	416:429	glycemic index	416:429	In this study, 17 wheat and 9 maize varieties were tested for starch composition, glycemic index, radical scavenging activities, and lipid profile.					
31368554	0	45	theme	glycemic	47:54	arg1	indices					56:62	glycemic indices	47:62	glycemic indices	47:62	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	2	46	theme	radical	432:438	arg1	activities					451:460	radical scavenging activities	432:460	radical scavenging activities	432:460	In this study, 17 wheat and 9 maize varieties were tested for starch composition, glycemic index, radical scavenging activities, and lipid profile.					
31368554	3	47	theme	highest	538:544	arg1	activity					594:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	3	48	theme	various	488:494	arg1	samples					502:508	various wheat samples	488:508	various wheat samples	488:508	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	6	49	theme	wheat	1044:1048	arg1	Saleem					1019:1024	Saleem	1019:1024	Saleem	1019:1024	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	6	49	theme	wheat	1044:1048	arg1	varieties					1050:1058	the best wheat varieties	1035:1058	the best wheat varieties	1035:1058	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	6	49	theme	wheat	1044:1048	arg1	KPK15					1009:1013	KPK15	1009:1013	KPK15	1009:1013	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	2	50	theme	scavenging	440:449	arg1	activities					451:460	radical scavenging activities	432:460	radical scavenging activities	432:460	In this study, 17 wheat and 9 maize varieties were tested for starch composition, glycemic index, radical scavenging activities, and lipid profile.					
31368554	0	51	theme	available	126:134	arg1	composition					7:17	Starch composition	0:17	Starch composition	0:17	Starch composition, antioxidant potential, and glycemic indices of various varieties of Triticum aesitivum L. and Zea mays L. available in Pakistan.					
31368554	8	52	theme	high	1387:1390	arg1	stress					1402:1407	high oxidative stress	1387:1407	high oxidative stress	1387:1407	Diabetes is characterized by hyperglycemia, hyperlipidemia, and high oxidative stress.					
31368554	3	53	theme	wheat	496:500	arg1	samples					502:508	various wheat samples	488:508	various wheat samples	488:508	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	6	54	theme	best	1039:1042	arg1	Saleem					1019:1024	Saleem	1019:1024	Saleem	1019:1024	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	6	54	theme	best	1039:1042	arg1	varieties					1050:1058	the best wheat varieties	1035:1058	the best wheat varieties	1035:1058	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	6	54	theme	best	1039:1042	arg1	KPK15					1009:1013	KPK15	1009:1013	KPK15	1009:1013	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	5	55	theme	amylose	972:978	arg1	contents					980:987	amylose contents	972:987	amylose contents	972:987	In most of the varieties, a linear correlation was observed between glycemic index and amylose contents.					
31368554	7	56	theme	PRACTICAL	1171:1179	arg1	APPLICATIONS					1181:1192	PRACTICAL APPLICATIONS	1171:1192	PRACTICAL APPLICATIONS: Wheat and maize	1171:1209	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	7	56	theme	PRACTICAL	1171:1179	arg1	crops					1243:1247	the most widely used cereal crops	1215:1247	the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients	1215:1320	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	7	56	theme	PRACTICAL	1171:1179	arg1	maize					1205:1209	maize	1205:1209	maize	1205:1209	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	7	56	theme	PRACTICAL	1171:1179	arg1	Wheat					1195:1199	Wheat	1195:1199	Wheat	1195:1199	PRACTICAL APPLICATIONS: Wheat and maize are the most widely used cereal crops being consumed randomly both by normal individuals and diabetic patients.					
31368554	9	57	theme	diabetes	1486:1493	arg1	development					1459:1469	development	1459:1469	development	1459:1469	Diet and nutrition play an important role in the development and control of diabetes.					
31368554	9	57	theme	diabetes	1486:1493	arg1	control					1475:1481	control	1475:1481	control	1475:1481	Diet and nutrition play an important role in the development and control of diabetes.					
31368554	1	58	theme	Bread	190:194	arg1	L					186:186	Triticum aesitivum L	167:186	Triticum aesitivum L. (Bread wheat)	167:201	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	1	58	theme	Bread	190:194	arg1	wheat					196:200	Bread wheat	190:200	Bread wheat	190:200	Many varieties of Triticum aesitivum L. (Bread wheat) and Zea maize L. (maize) have been developed in Pakistan, which are randomly consumed by normal individuals and diabetic patients.					
31368554	2	59	theme	lipid	467:471	arg1	profile					473:479	lipid profile	467:479	lipid profile	467:479	In this study, 17 wheat and 9 maize varieties were tested for starch composition, glycemic index, radical scavenging activities, and lipid profile.					
31368554	3	60	theme	scavenging	583:592	arg1	activity					594:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity	538:601	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	5	61	theme	linear	913:918	arg1	correlation					920:930	a linear correlation	911:930	a linear correlation	911:930	In most of the varieties, a linear correlation was observed between glycemic index and amylose contents.					
31368554	4	62	from	70.76 ± 4.40	862:873	arg1	CHT1W					878:882	CHT1W	878:882	CHT1W	878:882	Glycemic index of wheat varieties varied between 62.83 ± 2.19 and 70.70 ± 2.82 in Hashim10 and Insaf, respectively, while for maize it ranged between 56.45 ± 1.95 in Sarhad white and 70.76 ± 4.40 in CHT1W.					
31368554	10	63	theme	diabetic	1578:1585	arg1	patients					1587:1594	diabetic patients	1578:1594	diabetic patients	1578:1594	In this research, we attempted to find out the best wheat and maize varieties for diabetic patients from locally grown genotypes.					
31368554	5	64	located	observed	936:943	arg1	most					888:891	most	888:891	most	888:891	In most of the varieties, a linear correlation was observed between glycemic index and amylose contents.					
31368554	5	64	located	observed	936:943	arg1	varieties					900:908	the varieties	896:908	the varieties	896:908	In most of the varieties, a linear correlation was observed between glycemic index and amylose contents.					
31368554	5	64	located	observed	936:943	arg2	correlation					920:930	a linear correlation	911:930	a linear correlation	911:930	In most of the varieties, a linear correlation was observed between glycemic index and amylose contents.					
31368554	8	65	theme	oxidative	1392:1400	arg1	stress					1402:1407	high oxidative stress	1387:1407	high oxidative stress	1387:1407	Diabetes is characterized by hyperglycemia, hyperlipidemia, and high oxidative stress.					
31368554	9	66	dep	development	1459:1469	arg1	the					1455:1457	the	1455:1457	the	1455:1457	Diet and nutrition play an important role in the development and control of diabetes.					
31368554	10	67	theme	best	1543:1546	arg1	wheat					1548:1552	wheat	1548:1552	wheat	1548:1552	In this research, we attempted to find out the best wheat and maize varieties for diabetic patients from locally grown genotypes.					
31368554	3	68	theme	highest	624:630	arg1	scavenger					640:648	highest radical scavenger	624:648	highest radical scavenger	624:648	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
31368554	6	69	theme	maize	1097:1101	arg1	samples					1103:1109	maize samples	1097:1109	maize samples	1097:1109	The data show that KPK15 and Saleem 2000 are the best wheat varieties while Sarhad white is the best among maize samples to be consumed by normal individuals and diabetic patients.					
31368554	3	70	theme	radical	632:638	arg1	scavenger					640:648	highest radical scavenger	624:648	highest radical scavenger	624:648	Among various wheat samples, KPK15 was observed to have highest 2,2-diphenyl-1picrylhydrazyl radical scavenging activity, while CZP132011 was highest radical scavenger among various maize samples.					
29786838	5	0	with	compatible	934:943	arg1	FG					950:951	FG	950:951	FG	950:951	The results confirmed that gluten is compatible with FG, OPCs, and LA, thereby leading to the formation of a more uniform, dense, and hydrophobic film.					
29786838	5	0	with	compatible	934:943	arg1	OPCs					954:957	OPCs	954:957	OPCs	954:957	The results confirmed that gluten is compatible with FG, OPCs, and LA, thereby leading to the formation of a more uniform, dense, and hydrophobic film.					
29786838	5	0	with	compatible	934:943	arg1	LA					964:965	LA	964:965	LA	964:965	The results confirmed that gluten is compatible with FG, OPCs, and LA, thereby leading to the formation of a more uniform, dense, and hydrophobic film.					
29786838	5	1	theme	dense	1020:1024	arg1	film					1043:1046	a more uniform, dense, and hydrophobic film	1004:1046	a more uniform, dense, and hydrophobic film	1004:1046	The results confirmed that gluten is compatible with FG, OPCs, and LA, thereby leading to the formation of a more uniform, dense, and hydrophobic film.					
29786838	2	2	theme	acid	318:321	arg1	effects					245:251	The effects	241:251	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV)	241:413	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	4	3	theme	gluten	691:696	arg1	film					698:701	the composite gluten film	677:701	the composite gluten film	677:701	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	2	4	theme	water	335:339	arg1	permeability					347:358	the water vapor permeability	331:358	the water vapor permeability (WVP)	331:364	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	4	theme	water	335:339	arg1	WVP					361:363	WVP	361:363	WVP	361:363	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	5	5	theme	hydrophobic	1031:1041	arg1	film					1043:1046	a more uniform, dense, and hydrophobic film	1004:1046	a more uniform, dense, and hydrophobic film	1004:1046	The results confirmed that gluten is compatible with FG, OPCs, and LA, thereby leading to the formation of a more uniform, dense, and hydrophobic film.					
29786838	4	6	theme	hydrophobicity	787:800	arg1	analysis					802:809	surface hydrophobicity analysis	779:809	surface hydrophobicity analysis	779:809	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	6	theme	hydrophobicity	787:800	arg1	microscopy					742:751	scanning electron microscopy	724:751	scanning electron microscopy	724:751	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	2	7	theme	lauric	311:316	arg1	acid					318:321	lauric acid	311:321	lauric acid (LA)	311:326	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	7	theme	lauric	311:316	arg1	LA					324:325	LA	324:325	LA	324:325	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	7	8	from	seasonings	1387:1396	arg1	production					1406:1415	food production	1401:1415	food production	1401:1415	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	5	9	theme	film	1043:1046	arg1	formation					991:999	the formation	987:999	the formation of a more uniform, dense, and hydrophobic film	987:1046	The results confirmed that gluten is compatible with FG, OPCs, and LA, thereby leading to the formation of a more uniform, dense, and hydrophobic film.					
29786838	4	10	theme	atomic	754:759	arg1	microscopy					742:751	scanning electron microscopy	724:751	scanning electron microscopy	724:751	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	10	theme	atomic	754:759	arg1	microscopy					767:776	atomic force microscopy	754:776	atomic force microscopy	754:776	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	11	theme	composite	681:689	arg1	film					698:701	the composite gluten film	677:701	the composite gluten film	677:701	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	7	12	theme	seasoning	1266:1274	arg1	capacity					1286:1293	seasoning packaging capacity	1266:1293	seasoning packaging capacity	1266:1293	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	7	13	theme	packaging	1276:1284	arg1	capacity					1286:1293	seasoning packaging capacity	1266:1293	seasoning packaging capacity	1266:1293	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	6	14	theme	film	1150:1153	arg1	POV					1105:1107	POV	1105:1107	POV	1105:1107	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	14	theme	film	1150:1153	arg1	value					1119:1123	acid value	1114:1123	acid value	1114:1123	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	14	theme	film	1150:1153	arg1	appearance					1093:1102	appearance	1093:1102	appearance	1093:1102	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	14	theme	film	1150:1153	arg1	properties					1081:1090	the preservation properties	1064:1090	the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging	1064:1192	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	3	15	theme	WVP	457:459	arg1	properties					461:470	the WVP properties	453:470	the WVP properties of the gluten films	453:490	FG and OPCs improved the WVP properties of the gluten films, whereas LA significantly improved the oxygen-barrier properties.					
29786838	5	16	theme	uniform	1011:1017	arg1	film					1043:1046	a more uniform, dense, and hydrophobic film	1004:1046	a more uniform, dense, and hydrophobic film	1004:1046	The results confirmed that gluten is compatible with FG, OPCs, and LA, thereby leading to the formation of a more uniform, dense, and hydrophobic film.					
29786838	6	17	theme	gluten	1143:1148	arg1	film					1150:1153	the composite gluten film	1129:1153	the composite gluten film for oil, salt, and vegetable packaging	1129:1192	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	8	18	used	used	1556:1559	arg2	film					1544:1547	the film	1540:1547	the film	1540:1547	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	8	18	used	used	1556:1559	arg2	material					1574:1581	packaging material	1564:1581	packaging material for seasonings in food production	1564:1615	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	4	19	theme	film	698:701	arg1	properties					663:672	the morphological, microstructural, and thermal properties	615:672	the morphological, microstructural, and thermal properties of the composite gluten film	615:701	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	1	20	theme	films	165:169	arg1	properties					144:153	the barrier and mechanical properties	117:153	properties	144:153	The aim of this investigation was to improve the barrier and mechanical properties of gluten films and further explore their application in the packaging of seasonings.					
29786838	6	21	dep	oil	1159:1161	arg1	packaging					1184:1192	packaging	1184:1192	packaging	1184:1192	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	4	22	theme	force	761:765	arg1	microscopy					742:751	scanning electron microscopy	724:751	scanning electron microscopy	724:751	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	22	theme	force	761:765	arg1	microscopy					767:776	atomic force microscopy	754:776	atomic force microscopy	754:776	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	0	23	theme	Edible	0:5	arg1	Gum-Phenolic-Lipid					7:24	Edible Gum-Phenolic-Lipid	0:24	Edible Gum-Phenolic-Lipid	0:24	Edible Gum-Phenolic-Lipid Incorporated Gluten Films for Food Packaging.					
29786838	8	24	theme	packaging	1564:1572	arg1	film					1544:1547	the film	1540:1547	the film	1540:1547	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	8	24	theme	packaging	1564:1572	arg1	material					1574:1581	packaging material	1564:1581	packaging material for seasonings in food production	1564:1615	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	4	25	theme	composite	576:584	arg1	film					586:589	The FG/OPCs/LA/GP composite film	558:589	The FG/OPCs/LA/GP composite film	558:589	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	26	dep	transform	820:828	arg1	infrared					830:837	infrared	830:837	transform infrared spectroscopy	820:850	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	27	theme	surface	779:785	arg1	analysis					802:809	surface hydrophobicity analysis	779:809	surface hydrophobicity analysis	779:809	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	27	theme	surface	779:785	arg1	microscopy					742:751	scanning electron microscopy	724:751	scanning electron microscopy	724:751	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	8	28	theme	PRACTICAL	1418:1426	arg1	APPLICATION					1428:1438	PRACTICAL APPLICATION	1418:1438	PRACTICAL APPLICATION The edible composite film	1418:1464	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	4	29	theme	thermal	655:661	arg1	properties					663:672	the morphological, microstructural, and thermal properties	615:672	the morphological, microstructural, and thermal properties of the composite gluten film	615:701	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	2	30	theme	mechanical	367:376	arg1	properties					378:387	mechanical properties	367:387	mechanical properties	367:387	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	7	31	theme	75-day	1302:1307	arg1	period					1317:1322	a 75-day storage period	1300:1322	a 75-day storage period	1300:1322	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	7	32	theme	packaging	1364:1372	arg1	material					1374:1381	a packaging material	1362:1381	a packaging material	1362:1381	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	1	33	theme	investigation	88:100	arg1	aim					76:78	The aim	72:78	The aim of this investigation	72:100	The aim of this investigation was to improve the barrier and mechanical properties of gluten films and further explore their application in the packaging of seasonings.					
29786838	2	34	from	effects	245:251	arg1	permeability					347:358	the water vapor permeability	331:358	the water vapor permeability (WVP)	331:364	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	34	from	effects	245:251	arg1	properties					378:387	mechanical properties	367:387	mechanical properties	367:387	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	34	from	effects	245:251	arg1	POV					410:412	POV	410:412	POV	410:412	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	34	from	effects	245:251	arg1	WVP					361:363	WVP	361:363	WVP	361:363	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	34	from	effects	245:251	arg1	value					403:407	peroxide value	394:407	peroxide value (POV)	394:413	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	7	35	theme	storage	1309:1315	arg1	period					1317:1322	a 75-day storage period	1300:1322	a 75-day storage period	1300:1322	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	0	36	theme	Gluten	39:44	arg1	Films					46:50	Gluten Films	39:50	Gluten Films	39:50	Edible Gum-Phenolic-Lipid Incorporated Gluten Films for Food Packaging.					
29786838	7	37	theme	capacity	1286:1293	arg1	degree					1256:1261	some degree	1251:1261	some degree of seasoning packaging capacity	1251:1293	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	4	38	theme	gravimetric	861:871	arg1	analysis					873:880	thermal gravimetric analysis	853:880	thermal gravimetric analysis	853:880	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	2	39	theme	oligomeric	275:284	arg1	OPCs					300:303	OPCs	300:303	OPCs	300:303	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	39	theme	oligomeric	275:284	arg1	procyanidins					286:297	oligomeric procyanidins	275:297	oligomeric procyanidins (OPCs)	275:304	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	40	theme	procyanidins	286:297	arg1	effects					245:251	The effects	241:251	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV)	241:413	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	8	41	dep	APPLICATION	1428:1438	arg1	film					1461:1464	The edible composite film	1440:1464	PRACTICAL APPLICATION The edible composite film	1418:1464	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	4	42	theme	thermal	853:859	arg1	analysis					873:880	thermal gravimetric analysis	853:880	thermal gravimetric analysis	853:880	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	7	43	theme	food	1401:1404	arg1	production					1406:1415	food production	1401:1415	food production	1401:1415	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	1	44	theme	gluten	158:163	arg1	films					165:169	gluten films	158:169	gluten films	158:169	The aim of this investigation was to improve the barrier and mechanical properties of gluten films and further explore their application in the packaging of seasonings.					
29786838	7	45	theme	gluten	1228:1233	arg1	film					1235:1238	The composite gluten film	1214:1238	The composite gluten film	1214:1238	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	4	46	dep	Fourier	812:818	arg1	transform					820:828	transform	820:828	transform infrared spectroscopy	820:850	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	6	47	theme	acid	1114:1117	arg1	properties					1081:1090	the preservation properties	1064:1090	the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging	1064:1192	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	47	theme	acid	1114:1117	arg1	value					1119:1123	acid value	1114:1123	acid value	1114:1123	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	4	48	dep	microscopy	742:751	arg1	analysis					873:880	thermal gravimetric analysis	853:880	thermal gravimetric analysis	853:880	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	48	dep	microscopy	742:751	arg1	Fourier					812:818	Fourier	812:818	Fourier transform infrared spectroscopy	812:850	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	6	49	from	changes	1053:1059	arg1	POV					1105:1107	POV	1105:1107	POV	1105:1107	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	49	from	changes	1053:1059	arg1	value					1119:1123	acid value	1114:1123	acid value	1114:1123	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	49	from	changes	1053:1059	arg1	appearance					1093:1102	appearance	1093:1102	appearance	1093:1102	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	49	from	changes	1053:1059	arg1	properties					1081:1090	the preservation properties	1064:1090	the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging	1064:1192	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	4	50	theme	FG/OPCs/LA/GP	562:574	arg1	film					586:589	The FG/OPCs/LA/GP composite film	558:589	The FG/OPCs/LA/GP composite film	558:589	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	2	51	theme	peroxide	394:401	arg1	POV					410:412	POV	410:412	POV	410:412	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	51	theme	peroxide	394:401	arg1	value					403:407	peroxide value	394:407	peroxide value (POV)	394:413	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	8	52	theme	composite	1451:1459	arg1	film					1461:1464	The edible composite film	1440:1464	PRACTICAL APPLICATION The edible composite film	1418:1464	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	4	53	theme	electron	733:740	arg1	microscopy					742:751	scanning electron microscopy	724:751	scanning electron microscopy	724:751	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	53	theme	electron	733:740	arg1	microscopy					767:776	atomic force microscopy	754:776	atomic force microscopy	754:776	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	53	theme	electron	733:740	arg1	analysis					802:809	surface hydrophobicity analysis	779:809	surface hydrophobicity analysis	779:809	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	2	54	theme	vapor	341:345	arg1	permeability					347:358	the water vapor permeability	331:358	the water vapor permeability (WVP)	331:364	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	54	theme	vapor	341:345	arg1	WVP					361:363	WVP	361:363	WVP	361:363	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	8	55	theme	edible	1444:1449	arg1	film					1461:1464	The edible composite film	1440:1464	PRACTICAL APPLICATION The edible composite film	1418:1464	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	3	56	theme	films	486:490	arg1	properties					461:470	the WVP properties	453:470	the WVP properties of the gluten films	453:490	FG and OPCs improved the WVP properties of the gluten films, whereas LA significantly improved the oxygen-barrier properties.					
29786838	8	57	theme	food	1601:1604	arg1	production					1606:1615	food production	1601:1615	food production	1601:1615	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	0	58	theme	Food	56:59	arg1	Packaging					61:69	Food Packaging	56:69	Food Packaging	56:69	Edible Gum-Phenolic-Lipid Incorporated Gluten Films for Food Packaging.					
29786838	6	59	theme	composite	1133:1141	arg1	film					1150:1153	the composite gluten film	1129:1153	the composite gluten film for oil, salt, and vegetable packaging	1129:1192	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	4	60	theme	morphological	619:631	arg1	properties					663:672	the morphological, microstructural, and thermal properties	615:672	the morphological, microstructural, and thermal properties of the composite gluten film	615:701	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	7	61	theme	composite	1218:1226	arg1	film					1235:1238	The composite gluten film	1214:1238	The composite gluten film	1214:1238	The composite gluten film maintained some degree of seasoning packaging capacity over a 75-day storage period, indicating its potential for uses as a packaging material for seasonings in food production.					
29786838	2	62	theme	flaxseed	256:263	arg1	FG					270:271	FG	270:271	FG	270:271	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	2	62	theme	flaxseed	256:263	arg1	gum					265:267	flaxseed gum	256:267	flaxseed gum (FG)	256:272	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	1	63	from	application	197:207	arg1	packaging					216:224	the packaging	212:224	the packaging of seasonings	212:238	The aim of this investigation was to improve the barrier and mechanical properties of gluten films and further explore their application in the packaging of seasonings.					
29786838	1	64	theme	barrier	121:127	arg1	properties					144:153	the barrier and mechanical properties	117:153	properties	144:153	The aim of this investigation was to improve the barrier and mechanical properties of gluten films and further explore their application in the packaging of seasonings.					
29786838	2	65	theme	gum	265:267	arg1	effects					245:251	The effects	241:251	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV)	241:413	The effects of flaxseed gum (FG), oligomeric procyanidins (OPCs), and lauric acid (LA) on the water vapor permeability (WVP), mechanical properties, and peroxide value (POV) were determined.					
29786838	3	66	theme	oxygen-barrier	531:544	arg1	properties					546:555	the oxygen-barrier properties	527:555	the oxygen-barrier properties	527:555	FG and OPCs improved the WVP properties of the gluten films, whereas LA significantly improved the oxygen-barrier properties.					
29786838	4	67	theme	microstructural	634:648	arg1	properties					663:672	the morphological, microstructural, and thermal properties	615:672	the morphological, microstructural, and thermal properties of the composite gluten film	615:701	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	6	68	dep	properties	1081:1090	arg1	POV					1105:1107	POV	1105:1107	POV	1105:1107	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	68	dep	properties	1081:1090	arg1	properties					1081:1090	the preservation properties	1064:1090	the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging	1064:1192	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	68	dep	properties	1081:1090	arg1	appearance					1093:1102	appearance	1093:1102	appearance	1093:1102	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	68	dep	properties	1081:1090	arg1	value					1119:1123	acid value	1114:1123	acid value	1114:1123	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	4	69	theme	scanning	724:731	arg1	microscopy					742:751	scanning electron microscopy	724:751	scanning electron microscopy	724:751	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	69	theme	scanning	724:731	arg1	microscopy					767:776	atomic force microscopy	754:776	atomic force microscopy	754:776	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	4	69	theme	scanning	724:731	arg1	analysis					802:809	surface hydrophobicity analysis	779:809	surface hydrophobicity analysis	779:809	The FG/OPCs/LA/GP composite film was then optimized, and the morphological, microstructural, and thermal properties of the composite gluten film were investigated by scanning electron microscopy, atomic force microscopy, surface hydrophobicity analysis; Fourier transform infrared spectroscopy; thermal gravimetric analysis, respectively.					
29786838	1	70	theme	mechanical	133:142	arg1	properties					144:153	the barrier and mechanical properties	117:153	properties	144:153	The aim of this investigation was to improve the barrier and mechanical properties of gluten films and further explore their application in the packaging of seasonings.					
29786838	6	71	theme	preservation	1068:1079	arg1	POV					1105:1107	POV	1105:1107	POV	1105:1107	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	71	theme	preservation	1068:1079	arg1	value					1119:1123	acid value	1114:1123	acid value	1114:1123	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	71	theme	preservation	1068:1079	arg1	appearance					1093:1102	appearance	1093:1102	appearance	1093:1102	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	6	71	theme	preservation	1068:1079	arg1	properties					1081:1090	the preservation properties	1064:1090	the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging	1064:1192	The changes in the preservation properties (appearance, POV, and acid value) of the composite gluten film for oil, salt, and vegetable packaging were also examined.					
29786838	1	72	theme	seasonings	229:238	arg1	packaging					216:224	the packaging	212:224	the packaging of seasonings	212:238	The aim of this investigation was to improve the barrier and mechanical properties of gluten films and further explore their application in the packaging of seasonings.					
29786838	8	73	from	seasonings	1587:1596	arg1	production					1606:1615	food production	1601:1615	food production	1601:1615	PRACTICAL APPLICATION The edible composite film will be produced in industry according to the data provided in our paper; the film can be used as packaging material for seasonings in food production.					
29786838	3	74	theme	gluten	479:484	arg1	films					486:490	the gluten films	475:490	the gluten films	475:490	FG and OPCs improved the WVP properties of the gluten films, whereas LA significantly improved the oxygen-barrier properties.					
31333125	7	0	theme	scavenging	1425:1434	arg1	evaluations					1436:1446	the radical scavenging evaluations	1413:1446	the radical scavenging evaluations	1413:1446	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	8	1	theme	RES	1615:1617	arg1	combined					1590:1597	combined	1590:1597	combined	1590:1597	Also, the combined uses of ZEO and RES into alginate films had the synergistic effects (P<0.05).					
31333125	4	2	theme	2,2-Diphenyl-1-picrylhydrazyl	851:879	arg1	ABTS					949:952	ABTS	949:952	ABTS	949:952	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	4	2	theme	2,2-Diphenyl-1-picrylhydrazyl	851:879	arg1	assays					955:960	2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays	851:960	2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays	851:960	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	6	3	theme	gas	1182:1184	arg1	analysis					1227:1234	gas chromatography-mass spectrometry (GC/MS) analysis	1182:1234	gas chromatography-mass spectrometry (GC/MS) analysis	1182:1234	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	9	4	theme	appropriate	1795:1805	arg1	option					1807:1812	the appropriate option	1791:1812	the appropriate option to postpone the oxidative reactions	1791:1848	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	9	4	theme	appropriate	1795:1805	arg1	films					1733:1737	alginate films	1724:1737	alginate films incorporated with the combination of ZEO and RES	1724:1786	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	8	5	theme	alginate	1624:1631	arg1	films					1633:1637	alginate films	1624:1637	alginate films	1624:1637	Also, the combined uses of ZEO and RES into alginate films had the synergistic effects (P<0.05).					
31333125	6	6	theme	ZEO	1253:1255	arg1	compounds					1240:1248	35 compounds	1237:1248	35 compounds of ZEO	1237:1255	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	4	7	theme	films	827:831	arg1	activities					813:822	antioxidant activities	801:822	antioxidant activities of films	801:831	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	8	8	theme	synergistic	1647:1657	arg1	P<0.05					1668:1673	P<0.05	1668:1673	P<0.05	1668:1673	Also, the combined uses of ZEO and RES into alginate films had the synergistic effects (P<0.05).					
31333125	8	8	theme	synergistic	1647:1657	arg1	effects					1659:1665	the synergistic effects	1643:1665	the synergistic effects (P<0.05)	1643:1674	Also, the combined uses of ZEO and RES into alginate films had the synergistic effects (P<0.05).					
31333125	1	9	theme	synthetic	206:214	arg1	polymers					216:223	synthetic polymers	206:223	synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants	206:368	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	2	10	theme	essential	558:566	arg1	ZEO					573:575	ZEO	573:575	ZEO	573:575	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	2	10	theme	essential	558:566	arg1	oil					568:570	Zataria multiflora essential oil	539:570	Zataria multiflora essential oil (ZEO)	539:576	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	0	11	theme	Alginate	117:124	arg1	Films					136:140	Sodium Alginate Bioactive Films	110:140	Sodium Alginate Bioactive Films	110:140	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	5	12	theme	sodium	1052:1057	arg1	films					1068:1072	sodium alginate films	1052:1072	sodium alginate films	1052:1072	Finally, the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to films in combination forms to evaluate their synergistic effects.					
31333125	1	13	theme	due	225:227	arg1	polymers					216:223	synthetic polymers	206:223	synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants	206:368	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	2	14	theme	Zataria	539:545	arg1	ZEO					573:575	ZEO	573:575	ZEO	573:575	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	2	14	theme	Zataria	539:545	arg1	oil					568:570	Zataria multiflora essential oil	539:570	Zataria multiflora essential oil (ZEO)	539:576	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	6	15	theme	essential	1369:1377	arg1	oil					1379:1381	essential oil	1369:1381	essential oil	1369:1381	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	5	16	theme	better	1019:1024	arg1	activities					1038:1047	better antioxidant activities	1019:1047	better antioxidant activities	1019:1047	Finally, the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to films in combination forms to evaluate their synergistic effects.					
31333125	7	17	theme	Butylated	1548:1556	arg1	hydroxytoluene					1558:1571	Butylated hydroxytoluene	1548:1571	Butylated hydroxytoluene (BHT)	1548:1577	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	7	17	theme	Butylated	1548:1556	arg1	BHT					1574:1576	BHT	1574:1576	BHT	1574:1576	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	6	18	theme	GC/MS	1220:1224	arg1	analysis					1227:1234	gas chromatography-mass spectrometry (GC/MS) analysis	1182:1234	gas chromatography-mass spectrometry (GC/MS) analysis	1182:1234	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	9	19	theme	natural	1869:1875	arg1	it					1699:1700	it	1699:1700	it	1699:1700	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	9	19	theme	natural	1869:1875	arg1	antioxidant					1877:1887	natural antioxidant	1869:1887	natural antioxidant in the food packaging industry	1869:1918	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	6	20	theme	spectrometry	1206:1217	arg1	analysis					1227:1234	gas chromatography-mass spectrometry (GC/MS) analysis	1182:1234	gas chromatography-mass spectrometry (GC/MS) analysis	1182:1234	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	8	21	dep	combined	1590:1597	arg1	uses					1599:1602	uses	1599:1602	uses	1599:1602	Also, the combined uses of ZEO and RES into alginate films had the synergistic effects (P<0.05).					
31333125	1	22	theme	food	323:326	arg1	additives					328:336	food additives	323:336	food additives	323:336	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	9	23	theme	alginate	1724:1731	arg1	option					1807:1812	the appropriate option	1791:1812	the appropriate option to postpone the oxidative reactions	1791:1848	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	9	23	theme	alginate	1724:1731	arg1	films					1733:1737	alginate films	1724:1737	alginate films incorporated with the combination of ZEO and RES	1724:1786	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	1	24	theme	Biodegradable	154:166	arg1	films					168:172	BACKGROUND Biodegradable films	143:172	BACKGROUND Biodegradable films	143:172	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	1	24	theme	Biodegradable	154:166	arg1	alternatives					190:201	appropriate alternatives	178:201	appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants	178:368	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	9	25	theme	food	1896:1899	arg1	industry					1911:1918	the food packaging industry	1892:1918	the food packaging industry	1892:1918	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	0	26	from	Evaluation	0:9	arg1	Films					136:140	Sodium Alginate Bioactive Films	110:140	Sodium Alginate Bioactive Films	110:140	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	4	27	theme	sodium	727:732	arg1	films					743:747	sodium alginate films	727:747	sodium alginate films	727:747	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	0	28	theme	Essential	93:101	arg1	Oil					103:105	Essential Oil	93:105	Essential Oil	93:105	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	4	29	theme	films	743:747	arg1	amount					695:700	the amount	691:700	the amount of phenolic compounds of sodium alginate films	691:747	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	4	29	theme	films	743:747	arg1	compounds					714:722	phenolic compounds	705:722	phenolic compounds	705:722	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	4	29	theme	films	743:747	arg1	films					743:747	sodium alginate films	727:747	sodium alginate films	727:747	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	2	30	theme	study	397:401	arg1	aim					385:387	The aim	381:387	The aim of this study	381:401	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	6	31	theme	oil	1379:1381	arg1	3-Carene					1315:1322	3-Carene	1315:1322	3-Carene (4.98%)	1315:1330	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	6	31	theme	oil	1379:1381	arg1	compounds					1356:1364	the most important compounds	1337:1364	the most important compounds of essential oil	1337:1381	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	6	31	theme	oil	1379:1381	arg1	Thymol					1277:1282	Thymol	1277:1282	Thymol (37.94%)	1277:1291	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	6	31	theme	oil	1379:1381	arg1	p-Cymene					1294:1301	p-Cymene	1294:1301	p-Cymene (9.50%)	1294:1309	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	2	32	with	combination	522:532	arg1	ZEO					573:575	ZEO	573:575	ZEO	573:575	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	2	32	with	combination	522:532	arg1	oil					568:570	Zataria multiflora essential oil	539:570	Zataria multiflora essential oil (ZEO)	539:576	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	2	33	theme	antioxidant	432:442	arg1	activities					444:453	the potential antioxidant activities	418:453	the potential antioxidant activities of sodium alginate films containing resveratrol (RES)	418:507	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	9	34	used	used	1861:1864	arg2	it					1699:1700	it	1699:1700	it	1699:1700	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	9	34	used	used	1861:1864	arg2	antioxidant					1877:1887	natural antioxidant	1869:1887	natural antioxidant in the food packaging industry	1869:1918	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	4	35	theme	compounds	714:722	arg1	amount					695:700	the amount	691:700	the amount of phenolic compounds of sodium alginate films	691:747	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	4	35	theme	compounds	714:722	arg1	compounds					714:722	phenolic compounds	705:722	phenolic compounds	705:722	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	4	35	theme	compounds	714:722	arg1	films					743:747	sodium alginate films	727:747	sodium alginate films	727:747	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	7	36	contain	containing	1499:1508	arg1	films					1493:1497	the films	1489:1497	the films containing RES	1489:1512	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	7	36	contain	containing	1499:1508	arg2	RES					1510:1512	RES	1510:1512	RES	1510:1512	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	3	37	theme	major	625:629	arg1	compositions					640:651	the major chemical compositions	621:651	the major chemical compositions of ZEO	621:658	METHODS At first, the major chemical compositions of ZEO were evaluated by GC/MS.					
31333125	3	37	theme	major	625:629	arg1	first					614:618	first	614:618	first	614:618	METHODS At first, the major chemical compositions of ZEO were evaluated by GC/MS.					
31333125	4	38	theme	contents	782:789	arg1	assay					791:795	total phenolic contents assay	767:795	total phenolic contents assay	767:795	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	6	39	theme	important	1346:1354	arg1	3-Carene					1315:1322	3-Carene	1315:1322	3-Carene (4.98%)	1315:1330	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	6	39	theme	important	1346:1354	arg1	compounds					1356:1364	the most important compounds	1337:1364	the most important compounds of essential oil	1337:1381	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	6	39	theme	important	1346:1354	arg1	Thymol					1277:1282	Thymol	1277:1282	Thymol (37.94%)	1277:1291	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	6	39	theme	important	1346:1354	arg1	p-Cymene					1294:1301	p-Cymene	1294:1301	p-Cymene (9.50%)	1294:1309	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	1	40	theme	wide	307:310	arg1	range					314:318	wide a range	307:318	wide a range of food additives	307:336	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	0	41	theme	Antioxidant	30:40	arg1	Effect					42:47	the Synergistic Antioxidant Effect	14:47	the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films	14:140	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	7	42	theme	antioxidant	1463:1473	arg1	activities					1475:1484	better antioxidant activities	1456:1484	better antioxidant activities of the films containing RES	1456:1512	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	5	43	theme	RES	1002:1004	arg1	concentrations					976:989	the concentrations	972:989	the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films	972:1072	Finally, the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to films in combination forms to evaluate their synergistic effects.					
31333125	2	44	theme	alginate	465:472	arg1	films					474:478	sodium alginate films	458:478	sodium alginate films containing resveratrol (RES)	458:507	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	3	45	theme	ZEO	656:658	arg1	compositions					640:651	the major chemical compositions	621:651	the major chemical compositions of ZEO	621:658	METHODS At first, the major chemical compositions of ZEO were evaluated by GC/MS.					
31333125	3	45	theme	ZEO	656:658	arg1	first					614:618	first	614:618	first	614:618	METHODS At first, the major chemical compositions of ZEO were evaluated by GC/MS.					
31333125	7	46	theme	evaluations	1436:1446	arg1	results					1402:1408	The results	1398:1408	The results	1398:1408	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	9	47	theme	ZEO	1776:1778	arg1	combination					1761:1771	the combination	1757:1771	the combination of ZEO and RES	1757:1786	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	0	48	from	Films	136:140	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.	0:141	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	4	49	theme	total	767:771	arg1	contents					782:789	total phenolic contents	767:789	total phenolic contents assay	767:795	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	7	50	theme	radical	1417:1423	arg1	evaluations					1436:1446	the radical scavenging evaluations	1413:1446	the radical scavenging evaluations	1413:1446	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	7	51	contain	containing	1529:1538	arg2	ZEO					1540:1542	ZEO	1540:1542	ZEO	1540:1542	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	7	51	contain	containing	1529:1538	arg1	films					1523:1527	the films	1519:1527	the films containing ZEO and Butylated hydroxytoluene (BHT)	1519:1577	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	7	51	contain	containing	1529:1538	arg2	BHT					1574:1576	BHT	1574:1576	BHT	1574:1576	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	7	51	contain	containing	1529:1538	arg2	hydroxytoluene					1558:1571	Butylated hydroxytoluene	1548:1571	Butylated hydroxytoluene (BHT)	1548:1577	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	9	52	theme	RES	1784:1786	arg1	combination					1761:1771	the combination	1757:1771	the combination of ZEO and RES	1757:1786	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	5	53	theme	synergistic	1140:1150	arg1	effects					1152:1158	their synergistic effects	1134:1158	their synergistic effects	1134:1158	Finally, the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to films in combination forms to evaluate their synergistic effects.					
31333125	2	54	theme	in	587:588	arg1	model					596:600	an in vitro model	584:600	an in vitro model	584:600	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	3	55	from	first	614:618	arg1	METHODS					603:609	METHODS	603:609	METHODS At first, the major chemical compositions of ZEO	603:658	METHODS At first, the major chemical compositions of ZEO were evaluated by GC/MS.					
31333125	1	56	theme	appropriate	178:188	arg1	films					168:172	BACKGROUND Biodegradable films	143:172	BACKGROUND Biodegradable films	143:172	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	1	56	theme	appropriate	178:188	arg1	alternatives					190:201	appropriate alternatives	178:201	appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants	178:368	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	4	57	theme	antioxidant	801:811	arg1	activities					813:822	antioxidant activities	801:822	antioxidant activities of films	801:831	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	9	58	theme	oxidative	1830:1838	arg1	reactions					1840:1848	the oxidative reactions	1826:1848	the oxidative reactions	1826:1848	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	0	59	theme	Bioactive	126:134	arg1	Films					136:140	Sodium Alginate Bioactive Films	110:140	Sodium Alginate Bioactive Films	110:140	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	2	60	contain	containing	480:489	arg2	resveratrol					491:501	resveratrol	491:501	resveratrol (RES)	491:507	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	2	60	contain	containing	480:489	arg1	films					474:478	sodium alginate films	458:478	sodium alginate films containing resveratrol (RES)	458:507	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	2	60	contain	containing	480:489	arg2	RES					504:506	RES	504:506	RES	504:506	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	2	61	theme	multiflora	547:556	arg1	ZEO					573:575	ZEO	573:575	ZEO	573:575	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	2	61	theme	multiflora	547:556	arg1	oil					568:570	Zataria multiflora essential oil	539:570	Zataria multiflora essential oil (ZEO)	539:576	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	5	62	theme	alginate	1059:1066	arg1	films					1068:1072	sodium alginate films	1052:1072	sodium alginate films	1052:1072	Finally, the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to films in combination forms to evaluate their synergistic effects.					
31333125	4	63	theme	3-ethylbenzothiazoline-6-sulfonic	908:940	arg1	2,2'-azino-bis					892:905	2,2'-azino-bis	892:905	2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)	892:946	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	4	63	theme	3-ethylbenzothiazoline-6-sulfonic	908:940	arg1	acid					942:945	3-ethylbenzothiazoline-6-sulfonic acid	908:945	3-ethylbenzothiazoline-6-sulfonic acid	908:945	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	9	64	dep	CONCLUSION	1677:1686	arg1	concluded					1709:1717	concluded	1709:1717	can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions	1702:1848	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	9	64	dep	CONCLUSION	1677:1686	arg1	used					1861:1864	used	1861:1864	can be used as natural antioxidant in the food packaging industry	1854:1918	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	2	65	dep	in	587:588	arg1	vitro					590:594	vitro	590:594	vitro	590:594	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	5	66	theme	antioxidant	1026:1036	arg1	activities					1038:1047	better antioxidant activities	1019:1047	better antioxidant activities	1019:1047	Finally, the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to films in combination forms to evaluate their synergistic effects.					
31333125	1	67	theme	BACKGROUND	143:152	arg1	films					168:172	BACKGROUND Biodegradable films	143:172	BACKGROUND Biodegradable films	143:172	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	1	67	theme	BACKGROUND	143:152	arg1	alternatives					190:201	appropriate alternatives	178:201	appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants	178:368	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	6	68	theme	chromatography-mass	1186:1204	arg1	analysis					1227:1234	gas chromatography-mass spectrometry (GC/MS) analysis	1182:1234	gas chromatography-mass spectrometry (GC/MS) analysis	1182:1234	RESULTS According to gas chromatography-mass spectrometry (GC/MS) analysis, 35 compounds of ZEO were identified and Thymol (37.94%), p-Cymene (9.50%) and 3-Carene (4.98%) were the most important compounds of essential oil, respectively.					
31333125	0	69	theme	Boiss	87:91	arg1	Effect					42:47	the Synergistic Antioxidant Effect	14:47	the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films	14:140	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	1	70	theme	additives	328:336	arg1	range					314:318	wide a range	307:318	wide a range of food additives	307:336	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	4	71	theme	2,2'-azino-bis	892:905	arg1	ABTS					949:952	ABTS	949:952	ABTS	949:952	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	4	71	theme	2,2'-azino-bis	892:905	arg1	assays					955:960	2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays	851:960	2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays	851:960	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	9	72	theme	packaging	1901:1909	arg1	industry					1911:1918	the food packaging industry	1892:1918	the food packaging industry	1892:1918	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	1	73	theme	natural	349:355	arg1	antioxidants					357:368	natural antioxidants	349:368	natural antioxidants	349:368	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	7	74	theme	films	1493:1497	arg1	activities					1475:1484	better antioxidant activities	1456:1484	better antioxidant activities of the films containing RES	1456:1512	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	0	75	theme	Sodium	110:115	arg1	Films					136:140	Sodium Alginate Bioactive Films	110:140	Sodium Alginate Bioactive Films	110:140	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	8	76	contain	had	1639:1641	arg1	combined					1590:1597	combined	1590:1597	combined	1590:1597	Also, the combined uses of ZEO and RES into alginate films had the synergistic effects (P<0.05).					
31333125	8	76	contain	had	1639:1641	arg2	P<0.05					1668:1673	P<0.05	1668:1673	P<0.05	1668:1673	Also, the combined uses of ZEO and RES into alginate films had the synergistic effects (P<0.05).					
31333125	8	76	contain	had	1639:1641	arg2	effects					1659:1665	the synergistic effects	1643:1665	the synergistic effects (P<0.05)	1643:1674	Also, the combined uses of ZEO and RES into alginate films had the synergistic effects (P<0.05).					
31333125	0	77	from	Effect	42:47	arg1	Films					136:140	Sodium Alginate Bioactive Films	110:140	Sodium Alginate Bioactive Films	110:140	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	2	78	dep	OBJECTIVE	371:379	arg1	was					403:405	was	403:405	was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model	403:600	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	5	79	from	films	1095:1099	arg1	combination					1104:1114	combination	1104:1114	combination forms to evaluate their synergistic effects	1104:1158	Finally, the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to films in combination forms to evaluate their synergistic effects.					
31333125	9	80	from	antioxidant	1877:1887	arg1	industry					1911:1918	the food packaging industry	1892:1918	the food packaging industry	1892:1918	CONCLUSION Therefore, it can be concluded that alginate films incorporated with the combination of ZEO and RES is the appropriate option to postpone the oxidative reactions and can be used as natural antioxidant in the food packaging industry.					
31333125	0	81	theme	Synergistic	18:28	arg1	Effect					42:47	the Synergistic Antioxidant Effect	14:47	the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films	14:140	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	2	82	theme	potential	422:430	arg1	activities					444:453	the potential antioxidant activities	418:453	the potential antioxidant activities of sodium alginate films containing resveratrol (RES)	418:507	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	5	83	dep	combination	1104:1114	arg1	forms					1116:1120	forms	1116:1120	forms to evaluate their synergistic effects	1116:1158	Finally, the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to films in combination forms to evaluate their synergistic effects.					
31333125	4	84	theme	phenolic	705:712	arg1	compounds					714:722	phenolic compounds	705:722	phenolic compounds	705:722	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	0	85	theme	Effect	42:47	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.	0:141	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	0	86	dep	Resveratrol	52:62	arg1	Oil					103:105	Essential Oil	93:105	Essential Oil	93:105	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	2	87	theme	films	474:478	arg1	activities					444:453	the potential antioxidant activities	418:453	the potential antioxidant activities of sodium alginate films containing resveratrol (RES)	418:507	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	4	88	theme	phenolic	773:780	arg1	contents					782:789	total phenolic contents	767:789	total phenolic contents assay	767:795	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	0	89	theme	Resveratrol	52:62	arg1	Effect					42:47	the Synergistic Antioxidant Effect	14:47	the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films	14:140	Evaluation of the Synergistic Antioxidant Effect of Resveratrol and Zataria multiflora Boiss Essential Oil in Sodium Alginate Bioactive Films.					
31333125	1	90	theme	a	312:312	arg1	range					314:318	wide a range	307:318	wide a range of food additives	307:336	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	2	91	theme	sodium	458:463	arg1	films					474:478	sodium alginate films	458:478	sodium alginate films containing resveratrol (RES)	458:507	OBJECTIVE The aim of this study was to compare the potential antioxidant activities of sodium alginate films containing resveratrol (RES) alone and in combination with Zataria multiflora essential oil (ZEO) using an in vitro model.					
31333125	5	92	theme	ZEO	994:996	arg1	concentrations					976:989	the concentrations	972:989	the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films	972:1072	Finally, the concentrations of ZEO and RES which showed better antioxidant activities in sodium alginate films were incorporated to films in combination forms to evaluate their synergistic effects.					
31333125	7	93	theme	better	1456:1461	arg1	activities					1475:1484	better antioxidant activities	1456:1484	better antioxidant activities of the films containing RES	1456:1512	The results of the radical scavenging evaluations, showed better antioxidant activities of the films containing RES than the films containing ZEO and Butylated hydroxytoluene (BHT).					
31333125	4	94	theme	alginate	734:741	arg1	films					743:747	sodium alginate films	727:747	sodium alginate films	727:747	Then, the amount of phenolic compounds of sodium alginate films were evaluated by total phenolic contents assay and antioxidant activities of films were evaluated by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays.					
31333125	3	95	theme	chemical	631:638	arg1	compositions					640:651	the major chemical compositions	621:651	the major chemical compositions of ZEO	621:658	METHODS At first, the major chemical compositions of ZEO were evaluated by GC/MS.					
31333125	3	95	theme	chemical	631:638	arg1	first					614:618	first	614:618	first	614:618	METHODS At first, the major chemical compositions of ZEO were evaluated by GC/MS.					
31333125	8	96	theme	ZEO	1607:1609	arg1	combined					1590:1597	combined	1590:1597	combined	1590:1597	Also, the combined uses of ZEO and RES into alginate films had the synergistic effects (P<0.05).					
31333125	1	97	theme	food	259:262	arg1	carrier					295:301	a carrier	293:301	a carrier for wide a range of food additives	293:336	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	1	97	theme	food	259:262	arg1	safety					264:269	food safety	259:269	food safety	259:269	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
31333125	1	97	theme	food	259:262	arg1	function					281:288	their function	275:288	their function	275:288	BACKGROUND Biodegradable films are appropriate alternatives to synthetic polymers due to their potential to enhance food safety and their function as a carrier for wide a range of food additives especially natural antioxidants.					
29597877	20	0	theme	pathways	3011:3018	arg1	M					2979:2979	So M	2976:2979	So M	2976:2979	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	20	0	theme	pathways	3011:3018	arg1	measure					3036:3042	a measure	3034:3042	a measure solely of pathologic gut damage	3034:3074	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	20	0	theme	pathways	3011:3018	arg1	measure					2996:3002	a composite measure	2984:3002	a composite measure of all pathways	2984:3018	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	20	0	theme	pathways	3011:3018	arg1	L					3025:3025	L	3025:3025	L	3025:3025	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	10	1	dep	mannitol	1557:1564	arg1	lactulose					1688:1696	lactulose	1688:1696	this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose	1534:1696	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	10	1	dep	mannitol	1557:1564	arg1	lactulose					1566:1574	lactulose	1566:1574	this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose	1534:1696	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	14	2	theme	test	2290:2293	arg1	interpretation					2249:2262	the standard interpretation	2236:2262	the standard interpretation of the lactulose:mannitol test (L:M)	2236:2299	Impact statement Our work integrates the standard interpretation of the lactulose:mannitol test (L:M), with mechanistic insight of intestinal permeability.					
29597877	2	3	theme	mannitol	305:312	arg1	measure					338:344	a measure	336:344	a measure of normal small bowel function	336:375	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	2	3	theme	mannitol	305:312	arg1	absorption					314:323	mannitol absorption	305:323	mannitol absorption	305:323	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	3	4	theme	mannitol	501:508	arg1	test					510:513	the lactulose:mannitol test	487:513	the lactulose:mannitol test	487:513	A underappreciated limitation of this common understanding of the lactulose:mannitol test is that mannitol is not absorbed to any substantial extent by a transcellular process.					
29597877	18	5	theme	pore	2812:2815	arg1	pathways					2817:2824	pore pathways	2812:2824	pore pathways allowing only M to pass	2812:2848	Our observations are consistent with pore pathways allowing only M to pass.					
29597877	14	6	theme	intestinal	2330:2339	arg1	permeability					2341:2352	intestinal permeability	2330:2352	intestinal permeability	2330:2352	Impact statement Our work integrates the standard interpretation of the lactulose:mannitol test (L:M), with mechanistic insight of intestinal permeability.					
29597877	16	7	theme	differential	2573:2584	arg1	flux					2586:2589	the differential flux	2569:2589	the differential flux of L and M through the pathways	2569:2621	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	10	8	theme	leak	1507:1510	arg1	pathways					1512:1519	more leak pathways	1502:1519	more leak pathways	1502:1519	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	3	9	theme	lactulose	491:499	arg1	test					510:513	the lactulose:mannitol test	487:513	the lactulose:mannitol test	487:513	A underappreciated limitation of this common understanding of the lactulose:mannitol test is that mannitol is not absorbed to any substantial extent by a transcellular process.					
29597877	1	10	theme	sugar	153:157	arg1	test					212:215	specifically the lactulose:mannitol test	176:215	specifically the lactulose:mannitol test	176:215	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	1	10	theme	sugar	153:157	arg1	test					170:173	The dual sugar absorption test	144:173	The dual sugar absorption test	144:173	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	6	11	dep	model	1110:1114	arg1	light					1089:1093	light	1089:1093	light	1089:1093	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	10	12	theme	differential	1539:1550	arg1	mannitol					1557:1564	this differential flux mannitol	1534:1564	this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose	1534:1696	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	13	13	from	testing	2158:2164	arg1	inflammation					2185:2196	subclinical gut inflammation	2169:2196	subclinical gut inflammation	2169:2196	We suggest using lactulose alone is as informative as lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation.					
29597877	19	14	theme	normal	2928:2933	arg1	pathways					2909:2916	leak pathways	2904:2916	leak pathways which are normal, regulated, cell-junctional adaptations	2904:2973	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	19	14	theme	normal	2928:2933	arg1	adaptations					2963:2973	normal, regulated, cell-junctional adaptations	2928:2973	normal, regulated, cell-junctional adaptations	2928:2973	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	21	15	theme	more	3113:3116	arg1	information					3118:3128	more information	3113:3128	more information about gut health than L:M	3113:3154	Using L alone as a probe will yield more information about gut health than L:M.					
29597877	3	16	theme	common	463:468	arg1	understanding					470:482	this common understanding	458:482	this common understanding of the lactulose:mannitol test	458:513	A underappreciated limitation of this common understanding of the lactulose:mannitol test is that mannitol is not absorbed to any substantial extent by a transcellular process.					
29597877	15	17	from	pathways	2384:2391	arg1	epithelium					2404:2413	the gut epithelium	2396:2413	the gut epithelium	2396:2413	There are three paracellular pathways in the gut epithelium; pore, leak, and unrestricted.					
29597877	0	18	from	test	58:61	arg1	children					81:88	rural Malawian children	66:88	rural Malawian children	66:88	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	8	19	theme	absorbed	1225:1232	arg1	lactulose					1234:1242	absorbed lactulose	1225:1242	absorbed lactulose	1225:1242	In our population, absorbed lactulose and mannitol are strongly correlated, r = 0.68 P <0.0001, suggesting lactulose and mannitol are traversing the gut barrier via the same pathways.					
29597877	5	20	theme	Pore	792:795	arg1	pathways					806:813	Pore and leak pathways	792:813	Pore and leak pathways	792:813	Pore and leak pathways are regulated biological constructions of the small bowel barrier, and unrestricted pathways represent micropathological damage.					
29597877	12	21	theme	equal	1873:1877	arg1	rate					1859:1862	a rate	1857:1862	a rate at least equal to that of lactulose	1857:1898	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	16	22	theme	measurements	2469:2480	arg1	thousands					2452:2460	thousands	2452:2460	thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability	2452:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	13	23	dep	suggest	2066:2072	arg1	informative					2102:2112	informative	2102:2112	informative	2102:2112	We suggest using lactulose alone is as informative as lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation.					
29597877	0	24	theme	intestinal	119:128	arg1	permeability					130:141	intestinal permeability	119:141	intestinal permeability	119:141	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	5	25	theme	small	861:865	arg1	barrier					873:879	the small bowel barrier	857:879	the small bowel barrier	857:879	Pore and leak pathways are regulated biological constructions of the small bowel barrier, and unrestricted pathways represent micropathological damage.					
29597877	16	26	theme	rural	2487:2491	arg1	children					2502:2509	rural Malawian children	2487:2509	rural Malawian children at risk for increased intestinal permeability	2487:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	5	27	theme	barrier	873:879	arg1	constructions					840:852	biological constructions	829:852	biological constructions of the small bowel barrier	829:879	Pore and leak pathways are regulated biological constructions of the small bowel barrier, and unrestricted pathways represent micropathological damage.					
29597877	13	28	theme	subclinical	2169:2179	arg1	inflammation					2185:2196	subclinical gut inflammation	2169:2196	subclinical gut inflammation	2169:2196	We suggest using lactulose alone is as informative as lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation.					
29597877	2	29	theme	function	368:375	arg1	measure					338:344	a measure	336:344	a measure of normal small bowel function	336:375	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	2	29	theme	function	368:375	arg1	absorption					314:323	mannitol absorption	305:323	mannitol absorption	305:323	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	4	30	theme	lactulose	639:647	arg1	mannitol					649:656	lactulose:mannitol	639:656	lactulose:mannitol	639:656	Additionally, this interpretation of lactulose:mannitol is not consistent with current understanding of paracellular pathways, where three pathway types exist: pore, leak, and unrestricted.					
29597877	3	31	theme	substantial	555:565	arg1	extent					567:572	any substantial extent	551:572	any substantial extent	551:572	A underappreciated limitation of this common understanding of the lactulose:mannitol test is that mannitol is not absorbed to any substantial extent by a transcellular process.					
29597877	5	32	theme	biological	829:838	arg1	constructions					840:852	biological constructions	829:852	biological constructions of the small bowel barrier	829:879	Pore and leak pathways are regulated biological constructions of the small bowel barrier, and unrestricted pathways represent micropathological damage.					
29597877	4	33	theme	mannitol	649:656	arg1	interpretation					621:634	this interpretation	616:634	this interpretation of lactulose:mannitol	616:656	Additionally, this interpretation of lactulose:mannitol is not consistent with current understanding of paracellular pathways, where three pathway types exist: pore, leak, and unrestricted.					
29597877	15	34	dep	pathways	2384:2391	arg1	pathways					2384:2391	three paracellular pathways	2365:2391	three paracellular pathways in the gut epithelium; pore, leak, and unrestricted	2365:2443	There are three paracellular pathways in the gut epithelium; pore, leak, and unrestricted.					
29597877	15	34	dep	pathways	2384:2391	arg1	leak					2422:2425	leak	2422:2425	leak	2422:2425	There are three paracellular pathways in the gut epithelium; pore, leak, and unrestricted.					
29597877	15	34	dep	pathways	2384:2391	arg1	unrestricted					2432:2443	unrestricted	2432:2443	unrestricted	2432:2443	There are three paracellular pathways in the gut epithelium; pore, leak, and unrestricted.					
29597877	15	34	dep	pathways	2384:2391	arg1	pore					2416:2419	pore	2416:2419	pore	2416:2419	There are three paracellular pathways in the gut epithelium; pore, leak, and unrestricted.					
29597877	12	35	theme	given	1840:1844	arg1	pathway					1846:1852	a given pathway	1838:1852	a given pathway	1838:1852	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	13	36	theme	absorption	2147:2156	arg1	testing					2158:2164	a sugar absorption testing	2139:2164	a sugar absorption testing in subclinical gut inflammation	2139:2196	We suggest using lactulose alone is as informative as lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation.					
29597877	17	37	theme	little	2676:2681	arg1	L					2683:2683	little L	2676:2683	little L	2676:2683	Our findings challenge the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L.					
29597877	13	38	dep	lactulose	2117:2125	arg1	mannitol					2127:2134	mannitol	2127:2134	lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation	2117:2196	We suggest using lactulose alone is as informative as lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation.					
29597877	0	39	theme	rural	66:70	arg1	children					81:88	rural Malawian children	66:88	rural Malawian children	66:88	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	15	40	theme	gut	2400:2402	arg1	epithelium					2404:2413	the gut epithelium	2396:2413	the gut epithelium	2396:2413	There are three paracellular pathways in the gut epithelium; pore, leak, and unrestricted.					
29597877	5	41	theme	micropathological	918:934	arg1	damage					936:941	micropathological damage	918:941	micropathological damage	918:941	Pore and leak pathways are regulated biological constructions of the small bowel barrier, and unrestricted pathways represent micropathological damage.					
29597877	13	42	theme	gut	2181:2183	arg1	inflammation					2185:2196	subclinical gut inflammation	2169:2196	subclinical gut inflammation	2169:2196	We suggest using lactulose alone is as informative as lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation.					
29597877	2	43	used	used	328:331	arg2	absorption					314:323	mannitol absorption	305:323	mannitol absorption	305:323	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	2	43	used	used	328:331	arg2	measure					338:344	a measure	336:344	a measure of normal small bowel function	336:375	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	7	44	theme	normalizing	1142:1152	arg1	method					1132:1137	An alternative method	1117:1137	An alternative method of normalizing for gut length utilizing autopsy data	1117:1190	An alternative method of normalizing for gut length utilizing autopsy data is described.					
29597877	17	45	theme	epithelial	2714:2723	arg1	barrier					2725:2731	a normal epithelial barrier	2705:2731	a normal epithelial barrier	2705:2731	Our findings challenge the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L.					
29597877	2	46	theme	normalizing	391:401	arg1	factor					403:408	normalizing factor	391:408	normalizing factor for lactulose	391:422	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	21	47	theme	gut	3136:3138	arg1	health					3140:3145	gut health	3136:3145	gut health than L:M	3136:3154	Using L alone as a probe will yield more information about gut health than L:M.					
29597877	0	48	theme	mannitol	49:56	arg1	test					58:61	mannitol test	49:61	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.	0:142	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	17	49	theme	normalizing	2749:2759	arg1	factor					2761:2766	a normalizing factor	2747:2766	a normalizing factor for L	2747:2772	Our findings challenge the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L.					
29597877	17	49	theme	normalizing	2749:2759	arg1	M					2742:2742	M	2742:2742	M	2742:2742	Our findings challenge the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L.					
29597877	0	50	theme	EB	0:1	arg1	Article					8:14	EB 2017 Article	0:14	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.	0:142	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	1	51	theme	gut	236:238	arg1	health					240:245	gut health	236:245	gut health	236:245	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	15	52	theme	paracellular	2371:2382	arg1	leak					2422:2425	leak	2422:2425	leak	2422:2425	There are three paracellular pathways in the gut epithelium; pore, leak, and unrestricted.					
29597877	15	52	theme	paracellular	2371:2382	arg1	pathways					2384:2391	three paracellular pathways	2365:2391	three paracellular pathways in the gut epithelium; pore, leak, and unrestricted	2365:2443	There are three paracellular pathways in the gut epithelium; pore, leak, and unrestricted.					
29597877	15	52	theme	paracellular	2371:2382	arg1	pore					2416:2419	pore	2416:2419	pore	2416:2419	There are three paracellular pathways in the gut epithelium; pore, leak, and unrestricted.					
29597877	6	53	theme	rural	1029:1033	arg1	children					1044:1051	622 young rural Malawian children	1019:1051	622 young rural Malawian children	1019:1051	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	17	54	dep	notions	2663:2669	arg1	M					2742:2742	M	2742:2742	M	2742:2742	Our findings challenge the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L.					
29597877	17	54	dep	notions	2663:2669	arg1	factor					2761:2766	a normalizing factor	2747:2766	a normalizing factor for L	2747:2772	Our findings challenge the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L.					
29597877	17	54	dep	notions	2663:2669	arg1	absorbed					2688:2695	absorbed	2688:2695	is absorbed through a normal epithelial barrier	2685:2731	Our findings challenge the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L.					
29597877	4	55	theme	paracellular	706:717	arg1	pathways					719:726	paracellular pathways	706:726	paracellular pathways	706:726	Additionally, this interpretation of lactulose:mannitol is not consistent with current understanding of paracellular pathways, where three pathway types exist: pore, leak, and unrestricted.					
29597877	10	56	theme	unrestricted	1619:1630	arg1	pathways					1632:1639	unrestricted pathways	1619:1639	unrestricted pathways	1619:1639	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	20	57	theme	composite	2986:2994	arg1	M					2979:2979	So M	2976:2979	So M	2976:2979	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	20	57	theme	composite	2986:2994	arg1	measure					2996:3002	a composite measure	2984:3002	a composite measure of all pathways	2984:3018	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	6	58	theme	622	1019:1021	arg1	children					1044:1051	622 young rural Malawian children	1019:1051	622 young rural Malawian children	1019:1051	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	2	59	theme	small	356:360	arg1	function					368:375	normal small bowel function	349:375	normal small bowel function	349:375	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	1	60	used	used	221:224	arg2	test					170:173	The dual sugar absorption test	144:173	The dual sugar absorption test	144:173	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	1	60	used	used	221:224	arg2	test					212:215	specifically the lactulose:mannitol test	176:215	specifically the lactulose:mannitol test	176:215	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	20	61	theme	damage	3069:3074	arg1	M					2979:2979	So M	2976:2979	So M	2976:2979	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	20	61	theme	damage	3069:3074	arg1	measure					3036:3042	a measure	3034:3042	a measure solely of pathologic gut damage	3034:3074	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	20	61	theme	damage	3069:3074	arg1	measure					2996:3002	a composite measure	2984:3002	a composite measure of all pathways	2984:3018	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	20	61	theme	damage	3069:3074	arg1	L					3025:3025	L	3025:3025	L	3025:3025	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	16	62	theme	L	2594:2594	arg1	flux					2586:2589	the differential flux	2569:2589	the differential flux of L and M through the pathways	2569:2621	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	19	63	theme	substantial	2860:2870	arg1	M					2889:2889	M	2889:2889	M	2889:2889	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	19	63	theme	substantial	2860:2870	arg1	L					2883:2883	L	2883:2883	L	2883:2883	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	19	63	theme	substantial	2860:2870	arg1	amounts					2872:2878	substantial amounts	2860:2878	substantial amounts of L and M	2860:2889	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	3	64	theme	transcellular	579:591	arg1	process					593:599	a transcellular process	577:599	a transcellular process	577:599	A underappreciated limitation of this common understanding of the lactulose:mannitol test is that mannitol is not absorbed to any substantial extent by a transcellular process.					
29597877	19	65	theme	M	2889:2889	arg1	M					2889:2889	M	2889:2889	M	2889:2889	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	19	65	theme	M	2889:2889	arg1	L					2883:2883	L	2883:2883	L	2883:2883	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	19	65	theme	M	2889:2889	arg1	amounts					2872:2878	substantial amounts	2860:2878	substantial amounts of L and M	2860:2889	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	12	66	theme	mannitol	2048:2055	arg1	test					2057:2060	the lactulose:mannitol test	2034:2060	the lactulose:mannitol test	2034:2060	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	19	67	theme	L	2883:2883	arg1	M					2889:2889	M	2889:2889	M	2889:2889	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	19	67	theme	L	2883:2883	arg1	L					2883:2883	L	2883:2883	L	2883:2883	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	19	67	theme	L	2883:2883	arg1	amounts					2872:2878	substantial amounts	2860:2878	substantial amounts of L and M	2860:2889	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	2	68	theme	gut	290:292	arg1	damage					294:299	gut damage	290:299	gut damage	290:299	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	16	69	theme	intestinal	2533:2542	arg1	permeability					2544:2555	increased intestinal permeability	2523:2555	increased intestinal permeability	2523:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	10	70	theme	mannitol	1679:1686	arg1	flux					1671:1674	the differential flux	1654:1674	the differential flux of mannitol	1654:1686	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	0	71	dep	Article	8:14	arg1	test					58:61	mannitol test	49:61	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.	0:142	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	0	71	dep	Article	8:14	arg1	Interpretation					17:30	Interpretation	17:30	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.	0:142	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	6	72	theme	gut	1075:1077	arg1	health					1079:1084	poor gut health	1070:1084	poor gut health	1070:1084	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	19	73	dep	normal	2928:2933	arg1	cell-junctional					2947:2961	cell-junctional	2947:2961	cell-junctional	2947:2961	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	19	73	dep	normal	2928:2933	arg1	regulated					2936:2944	regulated	2936:2944	regulated	2936:2944	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	0	74	from	perturbations	102:114	arg1	permeability					130:141	intestinal permeability	119:141	intestinal permeability	119:141	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	10	75	theme	more	1502:1505	arg1	pathways					1512:1519	more leak pathways	1502:1519	more leak pathways	1502:1519	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	2	76	theme	normal	349:354	arg1	function					368:375	normal small bowel function	349:375	normal small bowel function	349:375	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	3	77	theme	understanding	470:482	arg1	limitation					444:453	A underappreciated limitation	425:453	A underappreciated limitation of this common understanding of the lactulose:mannitol test	425:513	A underappreciated limitation of this common understanding of the lactulose:mannitol test is that mannitol is not absorbed to any substantial extent by a transcellular process.					
29597877	5	78	theme	leak	801:804	arg1	pathways					806:813	Pore and leak pathways	792:813	Pore and leak pathways	792:813	Pore and leak pathways are regulated biological constructions of the small bowel barrier, and unrestricted pathways represent micropathological damage.					
29597877	13	79	theme	using	2074:2078	arg1	lactulose					2080:2088	using lactulose	2074:2088	using lactulose alone	2074:2094	We suggest using lactulose alone is as informative as lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation.					
29597877	20	80	theme	pathologic	3054:3063	arg1	damage					3069:3074	pathologic gut damage	3054:3074	pathologic gut damage	3054:3074	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	16	81	theme	increased	2523:2531	arg1	permeability					2544:2555	increased intestinal permeability	2523:2555	increased intestinal permeability	2523:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	0	82	from	risk	93:96	arg1	test					58:61	mannitol test	49:61	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.	0:142	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	4	83	with	consistent	665:674	arg1	understanding					689:701	current understanding	681:701	current understanding	681:701	Additionally, this interpretation of lactulose:mannitol is not consistent with current understanding of paracellular pathways, where three pathway types exist: pore, leak, and unrestricted.					
29597877	10	84	theme	differential	1658:1669	arg1	flux					1671:1674	the differential flux	1654:1674	the differential flux of mannitol	1654:1686	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	18	85	theme	only	2835:2838	arg1	M					2840:2840	only M	2835:2840	only M	2835:2840	Our observations are consistent with pore pathways allowing only M to pass.					
29597877	12	86	theme	composite	1931:1939	arg1	absorption					1915:1924	lactulose absorption	1905:1924	lactulose absorption	1905:1924	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	12	86	theme	composite	1931:1939	arg1	measure					1941:1947	a composite measure	1929:1947	a composite measure of flux through both physiologic and pathologic pathways	1929:2004	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	6	87	theme	high	1056:1059	arg1	risk					1061:1064	high risk	1056:1064	high risk for poor gut health	1056:1084	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	14	88	theme	mannitol	2281:2288	arg1	test					2290:2293	the lactulose:mannitol test	2267:2293	the lactulose:mannitol test (L:M)	2267:2299	Impact statement Our work integrates the standard interpretation of the lactulose:mannitol test (L:M), with mechanistic insight of intestinal permeability.					
29597877	2	89	theme	Lactulose	248:256	arg1	absorption					258:267	Lactulose absorption	248:267	Lactulose absorption	248:267	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	14	90	theme	permeability	2341:2352	arg1	insight					2319:2325	mechanistic insight	2307:2325	mechanistic insight of intestinal permeability	2307:2352	Impact statement Our work integrates the standard interpretation of the lactulose:mannitol test (L:M), with mechanistic insight of intestinal permeability.					
29597877	11	91	theme	linear	1778:1783	arg1	growth					1785:1790	linear growth	1778:1790	linear growth	1778:1790	There was no substantial correlation between the lactulose:mannitol and linear growth.					
29597877	8	92	theme	r = 0.68	1282:1289	arg1	P					1291:1291	P	1291:1291	P	1291:1291	In our population, absorbed lactulose and mannitol are strongly correlated, r = 0.68 P <0.0001, suggesting lactulose and mannitol are traversing the gut barrier via the same pathways.					
29597877	14	93	theme	lactulose	2271:2279	arg1	test					2290:2293	the lactulose:mannitol test	2267:2293	the lactulose:mannitol test (L:M)	2267:2299	Impact statement Our work integrates the standard interpretation of the lactulose:mannitol test (L:M), with mechanistic insight of intestinal permeability.					
29597877	12	94	theme	lactulose	2038:2046	arg1	test					2057:2060	the lactulose:mannitol test	2034:2060	the lactulose:mannitol test	2034:2060	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	1	95	theme	dual	148:151	arg1	test					212:215	specifically the lactulose:mannitol test	176:215	specifically the lactulose:mannitol test	176:215	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	1	95	theme	dual	148:151	arg1	test					170:173	The dual sugar absorption test	144:173	The dual sugar absorption test	144:173	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	12	96	theme	lactulose	1905:1913	arg1	absorption					1915:1924	lactulose absorption	1905:1924	lactulose absorption	1905:1924	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	12	96	theme	lactulose	1905:1913	arg1	measure					1941:1947	a composite measure	1929:1947	a composite measure of flux through both physiologic and pathologic pathways	1929:2004	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	10	97	theme	flux	1552:1555	arg1	mannitol					1557:1564	this differential flux mannitol	1534:1564	this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose	1534:1696	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	12	98	theme	flux	1952:1955	arg1	absorption					1915:1924	lactulose absorption	1905:1924	lactulose absorption	1905:1924	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	12	98	theme	flux	1952:1955	arg1	measure					1941:1947	a composite measure	1929:1947	a composite measure of flux through both physiologic and pathologic pathways	1929:2004	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	1	99	theme	absorption	159:168	arg1	test					212:215	specifically the lactulose:mannitol test	176:215	specifically the lactulose:mannitol test	176:215	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	1	99	theme	absorption	159:168	arg1	test					170:173	The dual sugar absorption test	144:173	The dual sugar absorption test	144:173	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	16	100	theme	L	2465:2465	arg1	measurements					2469:2480	L:M measurements	2465:2480	L:M measurements from rural Malawian children at risk for increased intestinal permeability	2465:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	6	101	theme	pathway	1102:1108	arg1	model					1110:1114	the pathway model	1098:1114	the pathway model	1098:1114	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	14	102	theme	standard	2240:2247	arg1	interpretation					2249:2262	the standard interpretation	2236:2262	the standard interpretation of the lactulose:mannitol test (L:M)	2236:2299	Impact statement Our work integrates the standard interpretation of the lactulose:mannitol test (L:M), with mechanistic insight of intestinal permeability.					
29597877	3	103	theme	test	510:513	arg1	understanding					470:482	this common understanding	458:482	this common understanding of the lactulose:mannitol test	458:513	A underappreciated limitation of this common understanding of the lactulose:mannitol test is that mannitol is not absorbed to any substantial extent by a transcellular process.					
29597877	9	104	theme	pore	1421:1424	arg1	pathways					1426:1433	pore pathways	1421:1433	pore pathways	1421:1433	Considering measurements where pore pathways predominate, mannitol flux is about 14 times that of lactulose.					
29597877	21	105	dep	L	3152:3152	arg1	M					3154:3154	M	3154:3154	L:M	3152:3154	Using L alone as a probe will yield more information about gut health than L:M.					
29597877	16	106	theme	M	2467:2467	arg1	measurements					2469:2480	L:M measurements	2465:2480	L:M measurements from rural Malawian children at risk for increased intestinal permeability	2465:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	11	107	theme	substantial	1719:1729	arg1	correlation					1731:1741	no substantial correlation	1716:1741	no substantial correlation between the lactulose:mannitol and linear growth	1716:1790	There was no substantial correlation between the lactulose:mannitol and linear growth.					
29597877	16	108	theme	M	2600:2600	arg1	flux					2586:2589	the differential flux	2569:2589	the differential flux of L and M through the pathways	2569:2621	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	14	109	dep	L	2296:2296	arg1	M					2298:2298	M	2298:2298	L:M	2296:2298	Impact statement Our work integrates the standard interpretation of the lactulose:mannitol test (L:M), with mechanistic insight of intestinal permeability.					
29597877	19	110	dep	And	2851:2853	arg1	pass					2891:2894	pass	2891:2894	pass through leak pathways which are normal, regulated, cell-junctional adaptations	2891:2973	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	16	111	from	thousands	2452:2460	arg1	children					2502:2509	rural Malawian children	2487:2509	rural Malawian children at risk for increased intestinal permeability	2487:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	9	112	theme	mannitol	1448:1455	arg1	flux					1457:1460	mannitol flux	1448:1460	mannitol flux	1448:1460	Considering measurements where pore pathways predominate, mannitol flux is about 14 times that of lactulose.					
29597877	16	113	theme	Malawian	2493:2500	arg1	children					2502:2509	rural Malawian children	2487:2509	rural Malawian children at risk for increased intestinal permeability	2487:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	12	114	theme	physiologic	1970:1980	arg1	pathways					1997:2004	both physiologic and pathologic pathways	1965:2004	both physiologic and pathologic pathways	1965:2004	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	11	115	theme	lactulose	1755:1763	arg1	mannitol					1765:1772	the lactulose:mannitol	1751:1772	the lactulose:mannitol	1751:1772	There was no substantial correlation between the lactulose:mannitol and linear growth.					
29597877	5	116	theme	bowel	867:871	arg1	barrier					873:879	the small bowel barrier	857:879	the small bowel barrier	857:879	Pore and leak pathways are regulated biological constructions of the small bowel barrier, and unrestricted pathways represent micropathological damage.					
29597877	12	117	theme	pathologic	1986:1995	arg1	pathways					1997:2004	both physiologic and pathologic pathways	1965:2004	both physiologic and pathologic pathways	1965:2004	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	17	118	theme	normal	2707:2712	arg1	barrier					2725:2731	a normal epithelial barrier	2705:2731	a normal epithelial barrier	2705:2731	Our findings challenge the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L.					
29597877	7	119	theme	autopsy	1179:1185	arg1	data					1187:1190	autopsy data	1179:1190	autopsy data	1179:1190	An alternative method of normalizing for gut length utilizing autopsy data is described.					
29597877	6	120	theme	mannitol	971:978	arg1	measurements					980:991	2334 lactulose:mannitol measurements	956:991	2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health	956:1084	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	17	121	theme	traditional	2651:2661	arg1	notions					2663:2669	the traditional notions	2647:2669	the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L	2647:2772	Our findings challenge the traditional notions that little L is absorbed through a normal epithelial barrier and that M is a normalizing factor for L.					
29597877	7	122	theme	gut	1158:1160	arg1	length					1162:1167	gut length	1158:1167	gut length utilizing autopsy data	1158:1190	An alternative method of normalizing for gut length utilizing autopsy data is described.					
29597877	13	123	theme	sugar	2141:2145	arg1	testing					2158:2164	a sugar absorption testing	2139:2164	a sugar absorption testing in subclinical gut inflammation	2139:2196	We suggest using lactulose alone is as informative as lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation.					
29597877	6	124	theme	lactulose	961:969	arg1	measurements					980:991	2334 lactulose:mannitol measurements	956:991	2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health	956:1084	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	0	125	theme	Malawian	72:79	arg1	children					81:88	rural Malawian children	66:88	rural Malawian children	66:88	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	16	126	from	risk	2514:2517	arg1	children					2502:2509	rural Malawian children	2487:2509	rural Malawian children at risk for increased intestinal permeability	2487:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	9	127	theme	lactulose	1488:1496	arg1	times					1474:1478	about 14 times	1465:1478	about 14 times that of lactulose	1465:1496	Considering measurements where pore pathways predominate, mannitol flux is about 14 times that of lactulose.					
29597877	8	128	theme	P	1291:1291	arg1	<0.0001					1293:1299	r = 0.68 P <0.0001	1282:1299	r = 0.68 P <0.0001	1282:1299	In our population, absorbed lactulose and mannitol are strongly correlated, r = 0.68 P <0.0001, suggesting lactulose and mannitol are traversing the gut barrier via the same pathways.					
29597877	4	129	theme	pathways	719:726	arg1	understanding					689:701	current understanding	681:701	current understanding	681:701	Additionally, this interpretation of lactulose:mannitol is not consistent with current understanding of paracellular pathways, where three pathway types exist: pore, leak, and unrestricted.					
29597877	3	130	theme	underappreciated	427:442	arg1	limitation					444:453	A underappreciated limitation	425:453	A underappreciated limitation of this common understanding of the lactulose:mannitol test	425:513	A underappreciated limitation of this common understanding of the lactulose:mannitol test is that mannitol is not absorbed to any substantial extent by a transcellular process.					
29597877	16	131	from	children	2502:2509	arg1	measurements					2469:2480	L:M measurements	2465:2480	L:M measurements from rural Malawian children at risk for increased intestinal permeability	2465:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	16	131	from	children	2502:2509	arg1	thousands					2452:2460	thousands	2452:2460	thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability	2452:2555	Using thousands of L:M measurements from rural Malawian children at risk for increased intestinal permeability, we predict the differential flux of L and M through the pathways.					
29597877	0	132	theme	2017	3:6	arg1	Article					8:14	EB 2017 Article	0:14	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.	0:142	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	6	133	theme	Malawian	1035:1042	arg1	children					1044:1051	622 young rural Malawian children	1019:1051	622 young rural Malawian children	1019:1051	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	13	134	from	lactulose	2117:2125	arg1	testing					2158:2164	a sugar absorption testing	2139:2164	a sugar absorption testing in subclinical gut inflammation	2139:2196	We suggest using lactulose alone is as informative as lactulose:mannitol in a sugar absorption testing in subclinical gut inflammation.					
29597877	14	135	dep	test	2290:2293	arg1	L					2296:2296	L	2296:2296	L:M	2296:2298	Impact statement Our work integrates the standard interpretation of the lactulose:mannitol test (L:M), with mechanistic insight of intestinal permeability.					
29597877	4	136	theme	current	681:687	arg1	understanding					689:701	current understanding	681:701	current understanding	681:701	Additionally, this interpretation of lactulose:mannitol is not consistent with current understanding of paracellular pathways, where three pathway types exist: pore, leak, and unrestricted.					
29597877	5	137	theme	unrestricted	886:897	arg1	pathways					899:906	unrestricted pathways	886:906	unrestricted pathways	886:906	Pore and leak pathways are regulated biological constructions of the small bowel barrier, and unrestricted pathways represent micropathological damage.					
29597877	6	138	theme	young	1023:1027	arg1	children					1044:1051	622 young rural Malawian children	1019:1051	622 young rural Malawian children	1019:1051	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	9	139	dep	times	1474:1478	arg1	that					1480:1483	that	1480:1483	that	1480:1483	Considering measurements where pore pathways predominate, mannitol flux is about 14 times that of lactulose.					
29597877	2	140	theme	bowel	362:366	arg1	function					368:375	normal small bowel function	349:375	normal small bowel function	349:375	Lactulose absorption is said to represent gut damage and mannitol absorption is used as a measure of normal small bowel function and serves as normalizing factor for lactulose.					
29597877	14	141	theme	mechanistic	2307:2317	arg1	insight					2319:2325	mechanistic insight	2307:2325	mechanistic insight of intestinal permeability	2307:2352	Impact statement Our work integrates the standard interpretation of the lactulose:mannitol test (L:M), with mechanistic insight of intestinal permeability.					
29597877	10	142	theme	increased	1598:1606	arg1	numbers					1608:1614	increased numbers	1598:1614	increased numbers of unrestricted pathways	1598:1639	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	0	143	theme	lactulose	39:47	arg1	Interpretation					17:30	Interpretation	17:30	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.	0:142	EB 2017 Article: Interpretation of the lactulose:mannitol test in rural Malawian children at risk for perturbations in intestinal permeability.					
29597877	20	144	theme	gut	3065:3067	arg1	damage					3069:3074	pathologic gut damage	3054:3074	pathologic gut damage	3054:3074	So M is a composite measure of all pathways, and L is not a measure solely of pathologic gut damage.					
29597877	8	145	theme	gut	1355:1357	arg1	barrier					1359:1365	the gut barrier	1351:1365	the gut barrier	1351:1365	In our population, absorbed lactulose and mannitol are strongly correlated, r = 0.68 P <0.0001, suggesting lactulose and mannitol are traversing the gut barrier via the same pathways.					
29597877	12	146	theme	test	2057:2060	arg1	utility					2023:2029	the utility	2019:2029	the utility of the lactulose:mannitol test	2019:2060	Given that mannitol will always pass through a given pathway at a rate at least equal to that of lactulose, and lactulose absorption is a composite measure of flux through both physiologic and pathologic pathways, we question the utility of the lactulose:mannitol test.					
29597877	4	147	theme	pathway	741:747	arg1	types					749:753	three pathway types	735:753	three pathway types	735:753	Additionally, this interpretation of lactulose:mannitol is not consistent with current understanding of paracellular pathways, where three pathway types exist: pore, leak, and unrestricted.					
29597877	19	148	theme	leak	2904:2907	arg1	adaptations					2963:2973	normal, regulated, cell-junctional adaptations	2928:2973	normal, regulated, cell-junctional adaptations	2928:2973	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	19	148	theme	leak	2904:2907	arg1	pathways					2909:2916	leak pathways	2904:2916	leak pathways which are normal, regulated, cell-junctional adaptations	2904:2973	And that substantial amounts of L and M pass through leak pathways which are normal, regulated, cell-junctional adaptations.					
29597877	10	149	theme	pathways	1632:1639	arg1	numbers					1608:1614	increased numbers	1598:1614	increased numbers of unrestricted pathways	1598:1639	As more leak pathways are present, this differential flux mannitol:lactulose falls to 8:1 and when increased numbers of unrestricted pathways are present, the differential flux of mannitol:lactulose is 6:1.					
29597877	1	150	theme	mannitol	203:210	arg1	test					212:215	specifically the lactulose:mannitol test	176:215	specifically the lactulose:mannitol test	176:215	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	1	150	theme	mannitol	203:210	arg1	test					170:173	The dual sugar absorption test	144:173	The dual sugar absorption test	144:173	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	1	151	theme	lactulose	193:201	arg1	test					212:215	specifically the lactulose:mannitol test	176:215	specifically the lactulose:mannitol test	176:215	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	1	151	theme	lactulose	193:201	arg1	test					170:173	The dual sugar absorption test	144:173	The dual sugar absorption test	144:173	The dual sugar absorption test, specifically the lactulose:mannitol test, is used to assess gut health.					
29597877	6	152	theme	poor	1070:1073	arg1	health					1079:1084	poor gut health	1070:1084	poor gut health	1070:1084	We analyzed 2334 lactulose:mannitol measurements rigorously collected from 622 young rural Malawian children at high risk for poor gut health in light of the pathway model.					
29597877	18	153	with	consistent	2796:2805	arg1	pathways					2817:2824	pore pathways	2812:2824	pore pathways allowing only M to pass	2812:2848	Our observations are consistent with pore pathways allowing only M to pass.					
29597877	8	154	theme	same	1375:1378	arg1	pathways					1380:1387	the same pathways	1371:1387	the same pathways	1371:1387	In our population, absorbed lactulose and mannitol are strongly correlated, r = 0.68 P <0.0001, suggesting lactulose and mannitol are traversing the gut barrier via the same pathways.					
29597877	7	155	theme	alternative	1120:1130	arg1	method					1132:1137	An alternative method	1117:1137	An alternative method of normalizing for gut length utilizing autopsy data	1117:1190	An alternative method of normalizing for gut length utilizing autopsy data is described.					
33655001	5	0	theme	detailed	759:766	arg1	procedure					768:776	a detailed procedure	757:776	a detailed procedure for collection and preparation of plant material, ultra-thin sectioning, specimen labeling and contrasting	757:883	It provides a detailed procedure for collection and preparation of plant material, ultra-thin sectioning, specimen labeling and contrasting.					
33655001	4	1	theme	epitopes	676:683	arg1	labeling					644:651	immunogold labeling	633:651	immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM)	633:742	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	7	2	theme	cell	1140:1143	arg1	components					1145:1154	other cell components	1134:1154	other cell components in plant and animal tissues	1134:1182	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	4	3	theme	high-resolution	689:703	arg1	TEM					739:741	TEM	739:741	TEM	739:741	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	4	3	theme	high-resolution	689:703	arg1	microscopy					727:736	high-resolution transmission electron microscopy	689:736	high-resolution transmission electron microscopy (TEM)	689:742	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	0	4	theme	Electron	72:79	arg1	Microscopy					81:90	Electron Microscopy	72:90	Electron Microscopy	72:90	Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy.					
33655001	3	5	theme	cell	493:496	arg1	scale					525:529	nanometer scale	515:529	nanometer scale	515:529	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	5	theme	cell	493:496	arg1	polysaccharides					561:575	the wall polysaccharides	552:575	the wall polysaccharides	552:575	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	5	theme	cell	493:496	arg1	composites					503:512	cell wall composites	493:512	cell wall composites (nanometer scale)	493:530	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	2	6	theme	cell	269:272	arg1	wall					241:244	The wall	237:244	The wall	237:244	The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development.					
33655001	2	6	theme	cell	269:272	arg1	composite					274:282	a very dynamic cell composite	254:282	a very dynamic cell composite	254:282	The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development.					
33655001	7	7	theme	other	1134:1138	arg1	components					1145:1154	other cell components	1134:1154	other cell components in plant and animal tissues	1134:1182	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	2	8	theme	dynamic	261:267	arg1	wall					241:244	The wall	237:244	The wall	237:244	The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development.					
33655001	2	8	theme	dynamic	261:267	arg1	composite					274:282	a very dynamic cell composite	254:282	a very dynamic cell composite	254:282	The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development.					
33655001	3	9	theme	small	479:483	arg1	size					485:488	the small size	475:488	the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions	475:606	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	10	theme	wall	424:427	arg1	components					429:438	cell wall components	419:438	cell wall components	419:438	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	11	theme	in	440:441	arg1	visualization					402:414	The visualization	398:414	The visualization of cell wall components in situ	398:446	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	7	12	theme	plant	1159:1163	arg1	tissues					1176:1182	plant and animal tissues	1159:1182	plant and animal tissues	1159:1182	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	6	13	theme	immunolabeling	889:902	arg1	workflow					914:921	An immunolabeling procedure workflow	886:921	An immunolabeling procedure workflow	886:921	An immunolabeling procedure workflow was optimized to obtain high efficiency of carbohydrates labeling for high-resolution TEM.					
33655001	3	14	theme	nanometer	515:523	arg1	scale					525:529	nanometer scale	515:529	nanometer scale	515:529	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	14	theme	nanometer	515:523	arg1	polysaccharides					561:575	the wall polysaccharides	552:575	the wall polysaccharides	552:575	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	14	theme	nanometer	515:523	arg1	composites					503:512	cell wall composites	493:512	cell wall composites (nanometer scale)	493:530	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	7	15	theme	cell	1053:1056	arg1	characteristics					1063:1077	plant cell wall characteristics	1047:1077	plant cell wall characteristics in various plant tissues	1047:1102	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	3	16	theme	composites	503:512	arg1	size					485:488	the small size	475:488	the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions	475:606	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	17	theme	diversity	539:547	arg1	size					485:488	the small size	475:488	the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions	475:606	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	6	18	theme	procedure	904:912	arg1	workflow					914:921	An immunolabeling procedure workflow	886:921	An immunolabeling procedure workflow	886:921	An immunolabeling procedure workflow was optimized to obtain high efficiency of carbohydrates labeling for high-resolution TEM.					
33655001	2	19	theme	cell	380:383	arg1	development					385:395	cell development	380:395	cell development	380:395	The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development.					
33655001	5	20	theme	plant	812:816	arg1	material					818:825	plant material	812:825	plant material	812:825	It provides a detailed procedure for collection and preparation of plant material, ultra-thin sectioning, specimen labeling and contrasting.					
33655001	7	21	theme	animal	1169:1174	arg1	tissues					1176:1182	plant and animal tissues	1159:1182	plant and animal tissues	1159:1182	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	1	22	theme	Plant	93:97	arg1	walls					104:108	Plant cell walls	93:108	Plant cell walls	93:108	Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane.					
33655001	1	23	theme	rigid	185:189	arg1	layer					191:195	a rigid layer	183:195	a rigid layer located outside of the plasma membrane	183:234	Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane.					
33655001	0	24	theme	Cell	23:26	arg1	Epitopes					33:40	Plant Cell Wall Epitopes	17:40	Plant Cell Wall Epitopes	17:40	Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy.					
33655001	6	25	theme	high-resolution	993:1007	arg1	TEM					1009:1011	high-resolution TEM	993:1011	high-resolution TEM	993:1011	An immunolabeling procedure workflow was optimized to obtain high efficiency of carbohydrates labeling for high-resolution TEM.					
33655001	1	26	theme	cell	99:102	arg1	walls					104:108	Plant cell walls	93:108	Plant cell walls	93:108	Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane.					
33655001	5	27	theme	ultra-thin	828:837	arg1	sectioning					839:848	ultra-thin sectioning	828:848	ultra-thin sectioning	828:848	It provides a detailed procedure for collection and preparation of plant material, ultra-thin sectioning, specimen labeling and contrasting.					
33655001	0	28	theme	Plant	17:21	arg1	Epitopes					33:40	Plant Cell Wall Epitopes	17:40	Plant Cell Wall Epitopes	17:40	Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy.					
33655001	3	29	theme	large	533:537	arg1	diversity					539:547	large diversity	533:547	large diversity of the wall polysaccharides	533:575	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	1	30	theme	located	197:203	arg1	layer					191:195	a rigid layer	183:195	a rigid layer located outside of the plasma membrane	183:234	Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane.					
33655001	0	31	theme	Epitopes	33:40	arg1	Visualization					0:12	Visualization	0:12	Visualization of Plant Cell Wall Epitopes	0:40	Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy.					
33655001	4	32	theme	cell	666:669	arg1	epitopes					676:683	different cell wall epitopes	656:683	different cell wall epitopes for high-resolution transmission electron microscopy (TEM)	656:742	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	7	33	theme	various	1082:1088	arg1	tissues					1096:1102	various plant tissues	1082:1102	various plant tissues	1082:1102	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	3	34	theme	polysaccharides	561:575	arg1	scale					525:529	nanometer scale	515:529	nanometer scale	515:529	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	34	theme	polysaccharides	561:575	arg1	interactions					595:606	their complex interactions	581:606	their complex interactions	581:606	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	34	theme	polysaccharides	561:575	arg1	diversity					539:547	large diversity	533:547	large diversity of the wall polysaccharides	533:575	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	34	theme	polysaccharides	561:575	arg1	polysaccharides					561:575	the wall polysaccharides	552:575	the wall polysaccharides	552:575	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	34	theme	polysaccharides	561:575	arg1	composites					503:512	cell wall composites	493:512	cell wall composites (nanometer scale)	493:530	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	35	theme	components	429:438	arg1	visualization					402:414	The visualization	398:414	The visualization of cell wall components in situ	398:446	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	4	36	theme	immunogold	633:642	arg1	labeling					644:651	immunogold labeling	633:651	immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM)	633:742	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	0	37	theme	Wall	28:31	arg1	Epitopes					33:40	Plant Cell Wall Epitopes	17:40	Plant Cell Wall Epitopes	17:40	Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy.					
33655001	6	38	theme	carbohydrates	966:978	arg1	efficiency					952:961	high efficiency	947:961	high efficiency of carbohydrates labeling for high-resolution TEM	947:1011	An immunolabeling procedure workflow was optimized to obtain high efficiency of carbohydrates labeling for high-resolution TEM.					
33655001	3	39	theme	cell	419:422	arg1	components					429:438	cell wall components	419:438	cell wall components	419:438	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	5	40	theme	material	818:825	arg1	preparation					797:807	preparation	797:807	preparation	797:807	It provides a detailed procedure for collection and preparation of plant material, ultra-thin sectioning, specimen labeling and contrasting.					
33655001	5	40	theme	material	818:825	arg1	collection					782:791	collection	782:791	collection	782:791	It provides a detailed procedure for collection and preparation of plant material, ultra-thin sectioning, specimen labeling and contrasting.					
33655001	5	40	theme	material	818:825	arg1	labeling					860:867	specimen labeling	851:867	specimen labeling	851:867	It provides a detailed procedure for collection and preparation of plant material, ultra-thin sectioning, specimen labeling and contrasting.					
33655001	5	40	theme	material	818:825	arg1	sectioning					839:848	ultra-thin sectioning	828:848	ultra-thin sectioning	828:848	It provides a detailed procedure for collection and preparation of plant material, ultra-thin sectioning, specimen labeling and contrasting.					
33655001	5	41	theme	specimen	851:858	arg1	labeling					860:867	specimen labeling	851:867	specimen labeling	851:867	It provides a detailed procedure for collection and preparation of plant material, ultra-thin sectioning, specimen labeling and contrasting.					
33655001	0	42	theme	Immunogold	48:57	arg1	Labeling					59:66	Immunogold Labeling	48:66	Immunogold Labeling for Electron Microscopy	48:90	Visualization of Plant Cell Wall Epitopes Using Immunogold Labeling for Electron Microscopy.					
33655001	7	43	from	components	1145:1154	arg1	tissues					1176:1182	plant and animal tissues	1159:1182	plant and animal tissues	1159:1182	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	3	44	dep	in	440:441	arg1	situ					443:446	situ	443:446	situ	443:446	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	7	45	theme	plant	1047:1051	arg1	characteristics					1063:1077	plant cell wall characteristics	1047:1077	plant cell wall characteristics in various plant tissues	1047:1102	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	2	46	theme	polysaccharide	319:332	arg1	interactions					334:345	complex polysaccharide interactions	311:345	complex polysaccharide interactions	311:345	The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development.					
33655001	7	47	theme	plant	1090:1094	arg1	tissues					1096:1102	various plant tissues	1082:1102	various plant tissues	1082:1102	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	3	48	theme	wall	556:559	arg1	polysaccharides					561:575	the wall polysaccharides	552:575	the wall polysaccharides	552:575	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	2	49	theme	complex	311:317	arg1	interactions					334:345	complex polysaccharide interactions	311:345	complex polysaccharide interactions	311:345	The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development.					
33655001	6	50	theme	high	947:950	arg1	efficiency					952:961	high efficiency	947:961	high efficiency of carbohydrates labeling for high-resolution TEM	947:1011	An immunolabeling procedure workflow was optimized to obtain high efficiency of carbohydrates labeling for high-resolution TEM.					
33655001	4	51	theme	electron	718:725	arg1	TEM					739:741	TEM	739:741	TEM	739:741	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	4	51	theme	electron	718:725	arg1	microscopy					727:736	high-resolution transmission electron microscopy	689:736	high-resolution transmission electron microscopy (TEM)	689:742	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	4	52	theme	different	656:664	arg1	epitopes					676:683	different cell wall epitopes	656:683	different cell wall epitopes for high-resolution transmission electron microscopy (TEM)	656:742	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	3	53	theme	interactions	595:606	arg1	size					485:488	the small size	475:488	the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions	475:606	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	4	54	theme	wall	671:674	arg1	epitopes					676:683	different cell wall epitopes	656:683	different cell wall epitopes for high-resolution transmission electron microscopy (TEM)	656:742	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	1	55	theme	different	121:129	arg1	polysaccharides					131:145	different polysaccharides	121:145	different polysaccharides	121:145	Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane.					
33655001	7	56	theme	wall	1058:1061	arg1	characteristics					1063:1077	plant cell wall characteristics	1047:1077	plant cell wall characteristics in various plant tissues	1047:1102	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	1	57	theme	structural	151:160	arg1	proteins					162:169	structural proteins	151:169	structural proteins	151:169	Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane.					
33655001	1	58	theme	plasma	220:225	arg1	membrane					227:234	the plasma membrane	216:234	the plasma membrane	216:234	Plant cell walls consist of different polysaccharides and structural proteins, which form a rigid layer located outside of the plasma membrane.					
33655001	3	59	theme	wall	498:501	arg1	scale					525:529	nanometer scale	515:529	nanometer scale	515:529	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	59	theme	wall	498:501	arg1	polysaccharides					561:575	the wall polysaccharides	552:575	the wall polysaccharides	552:575	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	3	59	theme	wall	498:501	arg1	composites					503:512	cell wall composites	493:512	cell wall composites (nanometer scale)	493:530	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	7	60	from	characteristics	1063:1077	arg1	tissues					1096:1102	various plant tissues	1082:1102	various plant tissues	1082:1102	This method was applied to study plant cell wall characteristics in various plant tissues but could also be applied for other cell components in plant and animal tissues.					
33655001	3	61	theme	complex	587:593	arg1	interactions					595:606	their complex interactions	581:606	their complex interactions	581:606	The visualization of cell wall components in situ is very challenging due to the small size of cell wall composites (nanometer scale), large diversity of the wall polysaccharides and their complex interactions.					
33655001	2	62	theme	various	351:357	arg1	modifications					359:371	various modifications	351:371	various modifications	351:371	The wall is also a very dynamic cell composite, which is characterized by complex polysaccharide interactions and various modifications during cell development.					
33655001	4	63	theme	transmission	705:716	arg1	TEM					739:741	TEM	739:741	TEM	739:741	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
33655001	4	63	theme	transmission	705:716	arg1	microscopy					727:736	high-resolution transmission electron microscopy	689:736	high-resolution transmission electron microscopy (TEM)	689:742	This protocol describes immunogold labeling of different cell wall epitopes for high-resolution transmission electron microscopy (TEM).					
31899235	0	0	theme	Nano	50:53	arg1	gel					98:100	Nano zero-valent iron/carbon/alginate composite gel	50:100	Nano zero-valent iron/carbon/alginate composite gel	50:100	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	9	1	from	general	1267:1273	arg1	efficient					1292:1300	efficient	1292:1300	efficient	1292:1300	In general, the material is efficient, recyclable and controllable, which provides a new idea for the treatment of chromium-containing wastewater.					
31899235	9	1	from	general	1267:1273	arg1	material					1280:1287	the material	1276:1287	the material	1276:1287	In general, the material is efficient, recyclable and controllable, which provides a new idea for the treatment of chromium-containing wastewater.					
31899235	3	2	dep	removal	521:527	arg1	VI					517:518	VI	517:518	VI	517:518	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	0	3	theme	iron/carbon/alginate	67:86	arg1	gel					98:100	Nano zero-valent iron/carbon/alginate composite gel	50:100	Nano zero-valent iron/carbon/alginate composite gel	50:100	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	5	4	theme	Cr	872:873	arg1	rate					864:867	the rate	860:867	the rate of Cr(VI) removal	860:885	Compared with activated carbon-alginate complex and pure alginate, the introduction of nanoscale zero-valent iron greatly accelerated the rate of Cr(VI) removal.					
31899235	0	5	dep	gel	98:100	arg1	Cr					26:27	An ion release controlled Cr	0:27	An ion release controlled Cr(VI) treatment agent	0:47	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	5	6	theme	iron	835:838	arg1	introduction					797:808	the introduction	793:808	the introduction of nanoscale zero-valent iron	793:838	Compared with activated carbon-alginate complex and pure alginate, the introduction of nanoscale zero-valent iron greatly accelerated the rate of Cr(VI) removal.					
31899235	0	7	theme	zero-valent	55:65	arg1	gel					98:100	Nano zero-valent iron/carbon/alginate composite gel	50:100	Nano zero-valent iron/carbon/alginate composite gel	50:100	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	4	8	theme	solution	650:657	arg1	150 mL					623:628	150 mL	623:628	150 mL of 20 mg L-1 Cr(VI) solution	623:657	For 150 mL of 20 mg L-1 Cr(VI) solution, the removal efficiency could reach 100% in 6 h (dosage: 2 g L-1).					
31899235	3	9	theme	Cr	514:515	arg1	removal					521:527	Cr(VI) removal	514:527	Cr(VI) removal	514:527	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	1	10	theme	iron/carbon	112:122	arg1	composite					124:132	A simple iron/carbon composite	103:132	A simple iron/carbon composite	103:132	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	5	11	theme	nanoscale	813:821	arg1	iron					835:838	nanoscale zero-valent iron	813:838	nanoscale zero-valent iron	813:838	Compared with activated carbon-alginate complex and pure alginate, the introduction of nanoscale zero-valent iron greatly accelerated the rate of Cr(VI) removal.					
31899235	8	12	theme	Cr	1106:1107	arg1	mechanism					1093:1101	The mechanism	1089:1101	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate	1089:1165	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	3	13	theme	optimal	491:497	arg1	value					537:541	pH value	534:541	pH value	534:541	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	3	13	theme	optimal	491:497	arg1	conditions					499:508	the optimal conditions	487:508	the optimal conditions for Cr(VI) removal	487:527	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	1	14	theme	ion	150:152	arg1	exchange					154:161	ion exchange	150:161	ion exchange	150:161	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	0	15	theme	composite	88:96	arg1	gel					98:100	Nano zero-valent iron/carbon/alginate composite gel	50:100	Nano zero-valent iron/carbon/alginate composite gel	50:100	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	5	16	theme	pure	778:781	arg1	alginate					783:790	pure alginate	778:790	pure alginate	778:790	Compared with activated carbon-alginate complex and pure alginate, the introduction of nanoscale zero-valent iron greatly accelerated the rate of Cr(VI) removal.					
31899235	8	17	dep	Cr	1106:1107	arg1	removal					1113:1119	removal	1113:1119	Cr(VI) removal of iron/carbon composite coated with alginate	1106:1165	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	8	17	dep	Cr	1106:1107	arg1	VI					1109:1110	VI	1109:1110	VI	1109:1110	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	4	18	dep	reach	689:693	arg1	dosage					708:713	dosage	708:713	dosage: 2 g L-1	708:722	For 150 mL of 20 mg L-1 Cr(VI) solution, the removal efficiency could reach 100% in 6 h (dosage: 2 g L-1).					
31899235	0	19	theme	ion	3:5	arg1	Cr					26:27	An ion release controlled Cr	0:27	An ion release controlled Cr(VI) treatment agent	0:47	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	8	20	dep	steps	1194:1198	arg1	fixation					1228:1235	fixation	1228:1235	fixation	1228:1235	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	8	20	dep	steps	1194:1198	arg1	adsorption					1213:1222	the initial adsorption	1201:1222	the initial adsorption	1201:1222	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	8	20	dep	steps	1194:1198	arg1	reduction					1253:1261	subsequent reduction	1242:1261	subsequent reduction	1242:1261	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	8	20	dep	steps	1194:1198	arg1	steps					1194:1198	two steps	1190:1198	two steps: the initial adsorption and fixation, and subsequent reduction	1190:1261	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	2	21	dep	Cr	423:424	arg1	VI					426:427	VI	426:427	VI	426:427	Then above material was coated with alginate to form a stable gel, which combined adsorption and reduction to remove Cr(VI) efficiently and controllably.					
31899235	7	22	theme	ion	1023:1025	arg1	release					1007:1013	the release	1003:1013	the release of iron ion	1003:1025	Under pH 2, the release of iron ion increased linearly with time and reached 51.87 mg L-1 in 8 h.					
31899235	2	23	theme	stable	361:366	arg1	gel					368:370	a stable gel	359:370	a stable gel	359:370	Then above material was coated with alginate to form a stable gel, which combined adsorption and reduction to remove Cr(VI) efficiently and controllably.					
31899235	1	24	theme	large	262:266	arg1	iron					300:303	nanoscale zero-valent iron	278:303	nanoscale zero-valent iron	278:303	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	1	24	theme	large	262:266	arg1	amount					268:273	a large amount	260:273	a large amount of nanoscale zero-valent iron	260:303	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	9	25	theme	wastewater	1399:1408	arg1	treatment					1366:1374	the treatment	1362:1374	the treatment of chromium-containing wastewater	1362:1408	In general, the material is efficient, recyclable and controllable, which provides a new idea for the treatment of chromium-containing wastewater.					
31899235	4	26	theme	Cr	643:644	arg1	solution					650:657	20 mg L-1 Cr(VI) solution	633:657	20 mg L-1 Cr(VI) solution	633:657	For 150 mL of 20 mg L-1 Cr(VI) solution, the removal efficiency could reach 100% in 6 h (dosage: 2 g L-1).					
31899235	1	27	theme	carbothermal	167:178	arg1	reduction					180:188	carbothermal reduction	167:188	carbothermal reduction	167:188	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	0	28	theme	controlled	15:24	arg1	Cr					26:27	An ion release controlled Cr	0:27	An ion release controlled Cr(VI) treatment agent	0:47	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	8	29	theme	composite	1136:1144	arg1	Cr					1106:1107	Cr(VI) removal of iron/carbon composite coated with alginate	1106:1165	Cr(VI) removal of iron/carbon composite coated with alginate	1106:1165	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	8	30	theme	iron/carbon	1124:1134	arg1	composite					1136:1144	iron/carbon composite	1124:1144	iron/carbon composite	1124:1144	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	3	31	theme	pH	534:535	arg1	value					537:541	pH value	534:541	pH value	534:541	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	3	31	theme	pH	534:535	arg1	conditions					499:508	the optimal conditions	487:508	the optimal conditions for Cr(VI) removal	487:527	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	0	32	dep	agent	43:47	arg1	VI					29:30	VI	29:30	VI	29:30	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	0	33	theme	release	7:13	arg1	Cr					26:27	An ion release controlled Cr	0:27	An ion release controlled Cr(VI) treatment agent	0:47	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	4	34	theme	removal	664:670	arg1	efficiency					672:681	the removal efficiency	660:681	the removal efficiency	660:681	For 150 mL of 20 mg L-1 Cr(VI) solution, the removal efficiency could reach 100% in 6 h (dosage: 2 g L-1).					
31899235	4	35	dep	solution	650:657	arg1	VI					646:647	VI	646:647	VI	646:647	For 150 mL of 20 mg L-1 Cr(VI) solution, the removal efficiency could reach 100% in 6 h (dosage: 2 g L-1).					
31899235	9	36	theme	new	1349:1351	arg1	idea					1353:1356	a new idea	1347:1356	a new idea for the treatment of chromium-containing wastewater	1347:1408	In general, the material is efficient, recyclable and controllable, which provides a new idea for the treatment of chromium-containing wastewater.					
31899235	6	37	theme	adsorption	901:910	arg1	isotherm					912:919	adsorption isotherm	901:919	adsorption isotherm	901:919	In addition, adsorption isotherm and kinetics conformed to Freundlich and Elovich model, respectively.					
31899235	1	38	theme	nanoscale	278:286	arg1	iron					300:303	nanoscale zero-valent iron	278:303	nanoscale zero-valent iron	278:303	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	5	39	theme	activated	740:748	arg1	complex					766:772	activated carbon-alginate complex	740:772	activated carbon-alginate complex	740:772	Compared with activated carbon-alginate complex and pure alginate, the introduction of nanoscale zero-valent iron greatly accelerated the rate of Cr(VI) removal.					
31899235	3	40	dep	Cr	555:556	arg1	concentration					562:574	concentration	562:574	Cr(VI) concentration of 2	555:579	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	3	40	dep	Cr	555:556	arg1	VI					558:559	VI	558:559	VI	558:559	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	1	41	theme	zero-valent	288:298	arg1	iron					300:303	nanoscale zero-valent iron	278:303	nanoscale zero-valent iron	278:303	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	6	42	dep	Freundlich	947:956	arg1	model					970:974	model	970:974	model	970:974	In addition, adsorption isotherm and kinetics conformed to Freundlich and Elovich model, respectively.					
31899235	9	43	from	efficient	1292:1300	arg1	general					1267:1273	general	1267:1273	general	1267:1273	In general, the material is efficient, recyclable and controllable, which provides a new idea for the treatment of chromium-containing wastewater.					
31899235	5	44	dep	Cr	872:873	arg1	removal					879:885	removal	879:885	Cr(VI) removal	872:885	Compared with activated carbon-alginate complex and pure alginate, the introduction of nanoscale zero-valent iron greatly accelerated the rate of Cr(VI) removal.					
31899235	5	44	dep	Cr	872:873	arg1	VI					875:876	VI	875:876	VI	875:876	Compared with activated carbon-alginate complex and pure alginate, the introduction of nanoscale zero-valent iron greatly accelerated the rate of Cr(VI) removal.					
31899235	1	45	theme	iron	300:303	arg1	iron					300:303	nanoscale zero-valent iron	278:303	nanoscale zero-valent iron	278:303	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	1	45	theme	iron	300:303	arg1	amount					268:273	a large amount	260:273	a large amount of nanoscale zero-valent iron	260:303	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	0	46	dep	Cr	26:27	arg1	agent					43:47	(VI) treatment agent	28:47	An ion release controlled Cr(VI) treatment agent	0:47	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	9	47	theme	chromium-containing	1379:1397	arg1	wastewater					1399:1408	chromium-containing wastewater	1379:1408	chromium-containing wastewater	1379:1408	In general, the material is efficient, recyclable and controllable, which provides a new idea for the treatment of chromium-containing wastewater.					
31899235	8	48	theme	subsequent	1242:1251	arg1	reduction					1253:1261	subsequent reduction	1242:1261	subsequent reduction	1242:1261	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	8	48	theme	subsequent	1242:1251	arg1	steps					1194:1198	two steps	1190:1198	two steps: the initial adsorption and fixation, and subsequent reduction	1190:1261	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	4	49	from	%	698:698	arg1	6 h					703:705	6 h	703:705	6 h	703:705	For 150 mL of 20 mg L-1 Cr(VI) solution, the removal efficiency could reach 100% in 6 h (dosage: 2 g L-1).					
31899235	1	50	theme	simple	105:110	arg1	composite					124:132	A simple iron/carbon composite	103:132	A simple iron/carbon composite	103:132	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	5	51	theme	zero-valent	823:833	arg1	iron					835:838	nanoscale zero-valent iron	813:838	nanoscale zero-valent iron	813:838	Compared with activated carbon-alginate complex and pure alginate, the introduction of nanoscale zero-valent iron greatly accelerated the rate of Cr(VI) removal.					
31899235	1	52	theme	characterization	199:214	arg1	results					216:222	the characterization results	195:222	the characterization results	195:222	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	3	53	theme	2	579:579	arg1	value					537:541	pH value	534:541	pH value	534:541	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	3	53	theme	2	579:579	arg1	20 mg L-1					594:602	20 mg L-1	594:602	20 mg L-1	594:602	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	3	53	theme	2	579:579	arg1	dosage					544:549	dosage	544:549	dosage	544:549	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	3	53	theme	2	579:579	arg1	Cr					555:556	Cr(VI) concentration of 2	555:579	Cr(VI) concentration of 2	555:579	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	3	53	theme	2	579:579	arg1	conditions					499:508	the optimal conditions	487:508	the optimal conditions for Cr(VI) removal	487:527	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	3	53	theme	2	579:579	arg1	2 g L-1					582:588	2 g L-1	582:588	2 g L-1	582:588	The experiment showed that the optimal conditions for Cr(VI) removal were pH value, dosage and Cr(VI) concentration of 2, 2 g L-1 and 20 mg L-1, respectively.					
31899235	1	54	contain	contained	250:258	arg1	material					241:248	this material	236:248	this material	236:248	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	1	54	contain	contained	250:258	arg2	iron					300:303	nanoscale zero-valent iron	278:303	nanoscale zero-valent iron	278:303	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	1	54	contain	contained	250:258	arg2	amount					268:273	a large amount	260:273	a large amount of nanoscale zero-valent iron	260:303	A simple iron/carbon composite was prepared by ion exchange and carbothermal reduction, and the characterization results showed that this material contained a large amount of nanoscale zero-valent iron.					
31899235	2	55	theme	above	311:315	arg1	material					317:324	Then above material	306:324	Then above material	306:324	Then above material was coated with alginate to form a stable gel, which combined adsorption and reduction to remove Cr(VI) efficiently and controllably.					
31899235	4	56	theme	20 mg L-1	633:641	arg1	solution					650:657	20 mg L-1 Cr(VI) solution	633:657	20 mg L-1 Cr(VI) solution	633:657	For 150 mL of 20 mg L-1 Cr(VI) solution, the removal efficiency could reach 100% in 6 h (dosage: 2 g L-1).					
31899235	8	57	theme	initial	1205:1211	arg1	adsorption					1213:1222	the initial adsorption	1201:1222	the initial adsorption	1201:1222	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	8	57	theme	initial	1205:1211	arg1	steps					1194:1198	two steps	1190:1198	two steps: the initial adsorption and fixation, and subsequent reduction	1190:1261	The mechanism of Cr(VI) removal of iron/carbon composite coated with alginate might be controlled by two steps: the initial adsorption and fixation, and subsequent reduction.					
31899235	5	58	theme	carbon-alginate	750:764	arg1	complex					766:772	activated carbon-alginate complex	740:772	activated carbon-alginate complex	740:772	Compared with activated carbon-alginate complex and pure alginate, the introduction of nanoscale zero-valent iron greatly accelerated the rate of Cr(VI) removal.					
31899235	0	59	theme	treatment	33:41	arg1	agent					43:47	(VI) treatment agent	28:47	An ion release controlled Cr(VI) treatment agent	0:47	An ion release controlled Cr(VI) treatment agent: Nano zero-valent iron/carbon/alginate composite gel.					
31899235	4	60	dep	dosage	708:713	arg1	2 g L-1					716:722	2 g L-1	716:722	dosage: 2 g L-1	708:722	For 150 mL of 20 mg L-1 Cr(VI) solution, the removal efficiency could reach 100% in 6 h (dosage: 2 g L-1).					
31899235	7	61	theme	iron	1018:1021	arg1	ion					1023:1025	iron ion	1018:1025	iron ion	1018:1025	Under pH 2, the release of iron ion increased linearly with time and reached 51.87 mg L-1 in 8 h.					
29800672	0	0	theme	galls	69:73	arg1	composite					83:91	oak galls extract composite	65:91	oak galls extract composite	65:91	Skin protectant textiles loaded with fish collagen, chitosan and oak galls extract composite.					
29800672	2	1	theme	infectoria	419:428	arg1	OGE					438:440	OGE	438:440	OGE	438:440	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	2	1	theme	infectoria	419:428	arg1	galls					431:435	oak (Quercus infectoria) galls	406:435	oak (Quercus infectoria) galls (OGE)	406:441	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	3	2	theme	sensitive	644:652	arg1	strains					668:674	both antibiotic sensitive and resistant strains	628:674	both antibiotic sensitive and resistant strains	628:674	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	1	3	theme	pathogens	139:147	arg1	control					114:120	control	114:120	control	114:120	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	1	3	theme	pathogens	139:147	arg1	demands					170:176	highly important demands	153:176	highly important demands	153:176	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	1	3	theme	pathogens	139:147	arg1	Skin					94:97	Skin	94:97	Skin	94:97	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	6	4	theme	microbial	1113:1121	arg1	pathogens					1123:1131	all microbial pathogens	1109:1131	all microbial pathogens	1109:1131	Treated textiles upheld most of their antimicrobial activity after 2 laundering cycles toward all microbial pathogens.					
29800672	0	5	theme	oak	65:67	arg1	composite					83:91	oak galls extract composite	65:91	oak galls extract composite	65:91	Skin protectant textiles loaded with fish collagen, chitosan and oak galls extract composite.					
29800672	4	6	theme	cotton	800:805	arg1	fabrics					807:813	cotton fabrics	800:813	cotton fabrics	800:813	The entire agents were loaded onto cotton fabrics and evaluated for antimicrobial actions and durability.					
29800672	2	7	theme	fish	313:316	arg1	scales					290:295	scales	290:295	scales	290:295	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	2	7	theme	fish	313:316	arg1	skin					281:284	skin	281:284	skin	281:284	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	0	8	theme	extract	75:81	arg1	composite					83:91	oak galls extract composite	65:91	oak galls extract composite	65:91	Skin protectant textiles loaded with fish collagen, chitosan and oak galls extract composite.					
29800672	3	9	theme	antimicrobial	461:473	arg1	OGE					519:521	OGE	519:521	OGE	519:521	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	9	theme	antimicrobial	461:473	arg1	Cts					511:513	Cts	511:513	Cts	511:513	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	9	theme	antimicrobial	461:473	arg1	potentialities					475:488	The antimicrobial potentialities	457:488	The antimicrobial potentialities of extracted agents	457:508	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	10	theme	resistant	658:666	arg1	strains					668:674	both antibiotic sensitive and resistant strains	628:674	both antibiotic sensitive and resistant strains	628:674	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	5	11	with	treatments	972:981	arg1	Cts					996:998	Cts	996:998	Cts	996:998	Loaded textiles with the combined extracts' composite were the most effectual followed by individual treatments with OGE and Cts, respectively.					
29800672	5	11	with	treatments	972:981	arg1	OGE					988:990	OGE	988:990	OGE	988:990	Loaded textiles with the combined extracts' composite were the most effectual followed by individual treatments with OGE and Cts, respectively.					
29800672	3	12	theme	antibiotic	633:642	arg1	strains					668:674	both antibiotic sensitive and resistant strains	628:674	both antibiotic sensitive and resistant strains	628:674	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	5	13	with	textiles	878:885	arg1	composite					915:923	the combined extracts' composite	892:923	the combined extracts' composite	892:923	Loaded textiles with the combined extracts' composite were the most effectual followed by individual treatments with OGE and Cts, respectively.					
29800672	1	14	theme	important	160:168	arg1	demands					170:176	highly important demands	153:176	highly important demands	153:176	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	1	14	theme	important	160:168	arg1	control					114:120	control	114:120	control	114:120	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	1	14	theme	important	160:168	arg1	Skin					94:97	Skin	94:97	Skin	94:97	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	2	15	theme	Quercus	411:417	arg1	OGE					438:440	OGE	438:440	OGE	438:440	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	2	15	theme	Quercus	411:417	arg1	galls					431:435	oak (Quercus infectoria) galls	406:435	oak (Quercus infectoria) galls (OGE)	406:441	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	7	16	theme	fabrics	1226:1232	arg1	production					1183:1192	production	1183:1192	production of skin protectant and hygienic fabrics	1183:1232	This invention could be consequently applied for production of skin protectant and hygienic fabrics.					
29800672	3	17	theme	extracted	493:501	arg1	agents					503:508	extracted agents	493:508	extracted agents	493:508	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	6	18	theme	Treated	1015:1021	arg1	textiles					1023:1030	Treated textiles	1015:1030	Treated textiles	1015:1030	Treated textiles upheld most of their antimicrobial activity after 2 laundering cycles toward all microbial pathogens.					
29800672	0	19	theme	Skin	0:3	arg1	protectant					5:14	Skin protectant	0:14	Skin protectant	0:14	Skin protectant textiles loaded with fish collagen, chitosan and oak galls extract composite.					
29800672	3	20	theme	agents	503:508	arg1	OGE					519:521	OGE	519:521	OGE	519:521	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	20	theme	agents	503:508	arg1	Cts					511:513	Cts	511:513	Cts	511:513	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	20	theme	agents	503:508	arg1	potentialities					475:488	The antimicrobial potentialities	457:488	The antimicrobial potentialities of extracted agents	457:508	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	2	21	theme	shrimp	377:382	arg1	shells					384:389	shrimp shells	377:389	shrimp shells	377:389	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	5	22	theme	combined	896:903	arg1	extracts					905:912	the combined extracts'	892:913	the combined extracts' composite	892:923	Loaded textiles with the combined extracts' composite were the most effectual followed by individual treatments with OGE and Cts, respectively.					
29800672	1	23	theme	natural	179:185	arg1	agents					198:203	natural biological agents	179:203	natural biological agents	179:203	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	1	23	theme	natural	179:185	arg1	ideals					213:218	the ideals	209:218	the ideals for that	209:227	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	1	24	theme	biological	187:196	arg1	agents					198:203	natural biological agents	179:203	natural biological agents	179:203	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	1	24	theme	biological	187:196	arg1	ideals					213:218	the ideals	209:218	the ideals for that	209:227	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	0	25	theme	fish	37:40	arg1	collagen					42:49	fish collagen	37:49	fish collagen	37:49	Skin protectant textiles loaded with fish collagen, chitosan and oak galls extract composite.					
29800672	7	26	theme	hygienic	1217:1224	arg1	fabrics					1226:1232	skin protectant and hygienic fabrics	1197:1232	skin protectant and hygienic fabrics	1197:1232	This invention could be consequently applied for production of skin protectant and hygienic fabrics.					
29800672	5	27	theme	individual	961:970	arg1	treatments					972:981	individual treatments	961:981	individual treatments with OGE and Cts, respectively	961:1012	Loaded textiles with the combined extracts' composite were the most effectual followed by individual treatments with OGE and Cts, respectively.					
29800672	2	28	theme	oak	406:408	arg1	OGE					438:440	OGE	438:440	OGE	438:440	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	2	28	theme	oak	406:408	arg1	galls					431:435	oak (Quercus infectoria) galls	406:435	oak (Quercus infectoria) galls (OGE)	406:441	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	3	29	dep	proved	543:548	arg1	neither					677:683	neither	677:683	neither	677:683	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	4	30	theme	antimicrobial	833:845	arg1	actions					847:853	antimicrobial actions	833:853	antimicrobial actions	833:853	The entire agents were loaded onto cotton fabrics and evaluated for antimicrobial actions and durability.					
29800672	3	31	theme	skin	558:561	arg1	pathogens					563:571	skin pathogens	558:571	skin pathogens	558:571	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	31	theme	skin	558:561	arg1	aureus					589:594	Staphylococcus aureus	574:594	Staphylococcus aureus	574:594	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	31	theme	skin	558:561	arg1	albicans					608:615	Candida albicans	600:615	Candida albicans	600:615	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	31	theme	skin	558:561	arg1	strains					668:674	both antibiotic sensitive and resistant strains	628:674	both antibiotic sensitive and resistant strains	628:674	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	4	32	theme	entire	769:774	arg1	agents					776:781	The entire agents	765:781	The entire agents	765:781	The entire agents were loaded onto cotton fabrics and evaluated for antimicrobial actions and durability.					
29800672	2	33	theme	tilapia	305:311	arg1	fish					313:316	Nile tilapia fish	300:316	Nile tilapia fish (Oreochromis niloticus)	300:340	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	2	33	theme	tilapia	305:311	arg1	niloticus					331:339	Oreochromis niloticus	319:339	Oreochromis niloticus	319:339	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	1	34	theme	microbial	129:137	arg1	pathogens					139:147	its microbial pathogens	125:147	its microbial pathogens	125:147	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	2	35	theme	Nile	300:303	arg1	fish					313:316	Nile tilapia fish	300:316	Nile tilapia fish (Oreochromis niloticus)	300:340	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	2	35	theme	Nile	300:303	arg1	niloticus					331:339	Oreochromis niloticus	319:339	Oreochromis niloticus	319:339	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	3	36	theme	negative	692:699	arg1	control					701:707	negative control	692:707	negative control	692:707	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	37	theme	Candida	600:606	arg1	pathogens					563:571	skin pathogens	558:571	skin pathogens	558:571	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	37	theme	Candida	600:606	arg1	albicans					608:615	Candida albicans	600:615	Candida albicans	600:615	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	3	37	theme	Candida	600:606	arg1	strains					668:674	both antibiotic sensitive and resistant strains	628:674	both antibiotic sensitive and resistant strains	628:674	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	5	38	theme	Loaded	871:876	arg1	effectual					939:947	effectual	939:947	effectual	939:947	Loaded textiles with the combined extracts' composite were the most effectual followed by individual treatments with OGE and Cts, respectively.					
29800672	5	38	theme	Loaded	871:876	arg1	textiles					878:885	Loaded textiles	871:885	Loaded textiles with the combined extracts' composite	871:923	Loaded textiles with the combined extracts' composite were the most effectual followed by individual treatments with OGE and Cts, respectively.					
29800672	0	39	dep	textiles	16:23	arg1	loaded					25:30	loaded	25:30	textiles loaded with fish collagen, chitosan and oak galls extract composite	16:91	Skin protectant textiles loaded with fish collagen, chitosan and oak galls extract composite.					
29800672	2	40	theme	Oreochromis	319:329	arg1	fish					313:316	Nile tilapia fish	300:316	Nile tilapia fish (Oreochromis niloticus)	300:340	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	2	40	theme	Oreochromis	319:329	arg1	niloticus					331:339	Oreochromis niloticus	319:339	Oreochromis niloticus	319:339	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	6	41	theme	laundering	1084:1093	arg1	cycles					1095:1100	2 laundering cycles	1082:1100	2 laundering cycles toward all microbial pathogens	1082:1131	Treated textiles upheld most of their antimicrobial activity after 2 laundering cycles toward all microbial pathogens.					
29800672	3	42	theme	examined	748:755	arg1	strain					757:762	examined strain	748:762	examined strain	748:762	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	2	43	theme	galls	431:435	arg1	extract					395:401	extract	395:401	extract of oak (Quercus infectoria) galls (OGE)	395:441	Collagen (Cg) was extracted and characterized from skin and scales of Nile tilapia fish (Oreochromis niloticus), chitosan (Cts) was extracted from shrimp shells and extract of oak (Quercus infectoria) galls (OGE) was prepared.					
29800672	5	44	dep	effectual	939:947	arg1	followed					949:956	followed	949:956	followed by individual treatments with OGE and Cts, respectively	949:1012	Loaded textiles with the combined extracts' composite were the most effectual followed by individual treatments with OGE and Cts, respectively.					
29800672	6	45	theme	antimicrobial	1053:1065	arg1	activity					1067:1074	their antimicrobial activity	1047:1074	their antimicrobial activity	1047:1074	Treated textiles upheld most of their antimicrobial activity after 2 laundering cycles toward all microbial pathogens.					
29800672	3	46	theme	antimicrobial	719:731	arg1	actions					733:739	antimicrobial actions	719:739	antimicrobial actions toward examined strain	719:762	The antimicrobial potentialities of extracted agents, Cts and OGE, were qualitatively proved against skin pathogens, Staphylococcus aureus and Candida albicans, including both antibiotic sensitive and resistant strains, neither Cg nor negative control exhibited antimicrobial actions toward examined strain.					
29800672	7	47	theme	skin	1197:1200	arg1	fabrics					1226:1232	skin protectant and hygienic fabrics	1197:1232	skin protectant and hygienic fabrics	1197:1232	This invention could be consequently applied for production of skin protectant and hygienic fabrics.					
29800672	1	48	dep	Skin	94:97	arg1	protection					99:108	protection	99:108	protection	99:108	Skin protection and control of its microbial pathogens are highly important demands; natural biological agents are the ideals for that.					
29800672	7	49	theme	protectant	1202:1211	arg1	fabrics					1226:1232	skin protectant and hygienic fabrics	1197:1232	skin protectant and hygienic fabrics	1197:1232	This invention could be consequently applied for production of skin protectant and hygienic fabrics.					
31075334	0	0	theme	In	82:83	arg1	behavior					91:98	In vitro behavior	82:98	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.	0:147	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	10	1	located	observed	968:975	arg2	behavior					938:945	The swelling behavior	925:945	The swelling behavior of the composite	925:962	The swelling behavior of the composite was observed in phosphate buffer saline solution.					
31075334	10	1	located	observed	968:975	arg1	solution					1004:1011	phosphate buffer saline solution	980:1011	phosphate buffer saline solution	980:1011	The swelling behavior of the composite was observed in phosphate buffer saline solution.					
31075334	6	2	theme	thermal	650:656	arg1	stability					658:666	the thermal stability	646:666	the thermal stability of the composite	646:683	A thermogravimetric analysis showed a significant enhancement in the thermal stability of the composite.					
31075334	12	3	theme	Saos-2	1139:1144	arg1	behavior					1127:1134	the in vitro behavior	1114:1134	the in vitro behavior of Saos-2	1114:1144	A preliminary investigation on the in vitro behavior of Saos-2 was also performed.					
31075334	8	4	used	used	799:802	arg2	microscopy					784:793	atomic force microscopy	771:793	atomic force microscopy	771:793	Furthermore, atomic force microscopy was used to investigate the roughness of the surface.					
31075334	7	5	theme	thermal	739:745	arg1	stability					747:755	a higher thermal stability	730:755	a higher thermal stability	730:755	The existence of hydroxyapatite resulted in a higher thermal stability.					
31075334	10	6	theme	buffer	990:995	arg1	solution					1004:1011	phosphate buffer saline solution	980:1011	phosphate buffer saline solution	980:1011	The swelling behavior of the composite was observed in phosphate buffer saline solution.					
31075334	13	7	theme	tissue	1245:1250	arg1	material					1264:1271	a tissue engineering material	1243:1271	a tissue engineering material	1243:1271	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	13	7	theme	tissue	1245:1250	arg1	properties					1229:1238	excellent properties	1219:1238	excellent properties	1219:1238	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	13	7	theme	tissue	1245:1250	arg1	cytotoxicity					1195:1206	good cytotoxicity	1190:1206	good cytotoxicity	1190:1206	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	11	8	from	inferiority	1045:1055	arg1	behavior					1073:1080	the swelling behavior	1060:1080	the swelling behavior	1060:1080	The hydroxyapatite offered the inferiority on the swelling behavior.					
31075334	13	9	theme	good	1190:1193	arg1	material					1264:1271	a tissue engineering material	1243:1271	a tissue engineering material	1243:1271	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	13	9	theme	good	1190:1193	arg1	properties					1229:1238	excellent properties	1219:1238	excellent properties	1219:1238	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	13	9	theme	good	1190:1193	arg1	cytotoxicity					1195:1206	good cytotoxicity	1190:1206	good cytotoxicity	1190:1206	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	10	10	theme	composite	954:962	arg1	behavior					938:945	The swelling behavior	925:945	The swelling behavior of the composite	925:962	The swelling behavior of the composite was observed in phosphate buffer saline solution.					
31075334	5	11	theme	electron	475:482	arg1	microscopy					484:493	Scanning electron microscopy	466:493	Scanning electron microscopy	466:493	Scanning electron microscopy and energy dispersive analyses confirmed the morphological properties and dispersion.					
31075334	0	12	theme	human	103:107	arg1	cultures					139:146	human osteoblast-like cell (Saos-2) cultures	103:146	human osteoblast-like cell (Saos-2) cultures	103:146	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	4	13	theme	chitosan	451:458	arg1	film					460:463	the chitosan film	447:463	the chitosan film	447:463	Then, 10-30 wt% of hydroxyapatite was loaded into the chitosan film.					
31075334	3	14	theme	solution	343:350	arg1	pH					333:334	The pH	329:334	The pH of the solution	329:350	The pH of the solution was adjusted to 10 using ammonium hydroxide.					
31075334	0	15	from	waste	44:48	arg1	Development					0:10	Development	0:10	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.	0:147	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	6	16	theme	significant	619:629	arg1	enhancement					631:641	a significant enhancement	617:641	a significant enhancement	617:641	A thermogravimetric analysis showed a significant enhancement in the thermal stability of the composite.					
31075334	9	17	theme	hydroxyapatite	870:883	arg1	addition					858:865	the addition	854:865	the addition of hydroxyapatite	854:883	With the addition of hydroxyapatite, the roughness significantly increased.					
31075334	0	18	dep	In	82:83	arg1	vitro					85:89	vitro	85:89	vitro	85:89	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	5	19	theme	energy	499:504	arg1	analyses					517:524	energy dispersive analyses	499:524	energy dispersive analyses	499:524	Scanning electron microscopy and energy dispersive analyses confirmed the morphological properties and dispersion.					
31075334	3	20	theme	ammonium	377:384	arg1	hydroxide					386:394	ammonium hydroxide	377:394	ammonium hydroxide	377:394	The pH of the solution was adjusted to 10 using ammonium hydroxide.					
31075334	12	21	from	investigation	1097:1109	arg1	behavior					1127:1134	the in vitro behavior	1114:1134	the in vitro behavior of Saos-2	1114:1144	A preliminary investigation on the in vitro behavior of Saos-2 was also performed.					
31075334	12	22	theme	in	1118:1119	arg1	behavior					1127:1134	the in vitro behavior	1114:1134	the in vitro behavior of Saos-2	1114:1144	A preliminary investigation on the in vitro behavior of Saos-2 was also performed.					
31075334	2	23	theme	ambient	308:314	arg1	temperature					316:326	an ambient temperature	305:326	an ambient temperature	305:326	Eggshell waste and orthophosphoric acid were co-precipitated for 2 h at an ambient temperature.					
31075334	0	24	theme	cell	125:128	arg1	cultures					139:146	human osteoblast-like cell (Saos-2) cultures	103:146	human osteoblast-like cell (Saos-2) cultures	103:146	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	10	25	theme	saline	997:1002	arg1	solution					1004:1011	phosphate buffer saline solution	980:1011	phosphate buffer saline solution	980:1011	The swelling behavior of the composite was observed in phosphate buffer saline solution.					
31075334	1	26	theme	eggshell	199:206	arg1	waste					208:212	eggshell waste	199:212	eggshell waste	199:212	Hydroxyapatite was successfully synthesized using eggshell waste as a raw material.					
31075334	1	26	theme	eggshell	199:206	arg1	material					223:230	a raw material	217:230	a raw material	217:230	Hydroxyapatite was successfully synthesized using eggshell waste as a raw material.					
31075334	0	27	theme	hydroxyapatite	15:28	arg1	Development					0:10	Development	0:10	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.	0:147	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	0	28	theme	osteoblast-like	109:123	arg1	cultures					139:146	human osteoblast-like cell (Saos-2) cultures	103:146	human osteoblast-like cell (Saos-2) cultures	103:146	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	12	29	dep	in	1118:1119	arg1	vitro					1121:1125	vitro	1121:1125	vitro	1121:1125	A preliminary investigation on the in vitro behavior of Saos-2 was also performed.					
31075334	0	30	theme	Saos-2	131:136	arg1	cultures					139:146	human osteoblast-like cell (Saos-2) cultures	103:146	human osteoblast-like cell (Saos-2) cultures	103:146	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	7	31	theme	higher	732:737	arg1	stability					747:755	a higher thermal stability	730:755	a higher thermal stability	730:755	The existence of hydroxyapatite resulted in a higher thermal stability.					
31075334	2	32	theme	Eggshell	233:240	arg1	waste					242:246	Eggshell waste	233:246	Eggshell waste	233:246	Eggshell waste and orthophosphoric acid were co-precipitated for 2 h at an ambient temperature.					
31075334	5	33	theme	Scanning	466:473	arg1	microscopy					484:493	Scanning electron microscopy	466:493	Scanning electron microscopy	466:493	Scanning electron microscopy and energy dispersive analyses confirmed the morphological properties and dispersion.					
31075334	0	34	theme	eggshell	35:42	arg1	waste					44:48	eggshell waste	35:48	eggshell waste	35:48	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	8	35	theme	atomic	771:776	arg1	microscopy					784:793	atomic force microscopy	771:793	atomic force microscopy	771:793	Furthermore, atomic force microscopy was used to investigate the roughness of the surface.					
31075334	6	36	theme	thermogravimetric	583:599	arg1	analysis					601:608	A thermogravimetric analysis	581:608	A thermogravimetric analysis	581:608	A thermogravimetric analysis showed a significant enhancement in the thermal stability of the composite.					
31075334	0	37	from	composite	71:79	arg1	Development					0:10	Development	0:10	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.	0:147	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	5	38	theme	morphological	540:552	arg1	properties					554:563	the morphological properties	536:563	the morphological properties	536:563	Scanning electron microscopy and energy dispersive analyses confirmed the morphological properties and dispersion.					
31075334	0	39	theme	cultures	139:146	arg1	behavior					91:98	In vitro behavior	82:98	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.	0:147	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	13	40	theme	excellent	1219:1227	arg1	material					1264:1271	a tissue engineering material	1243:1271	a tissue engineering material	1243:1271	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	13	40	theme	excellent	1219:1227	arg1	properties					1229:1238	excellent properties	1219:1238	excellent properties	1219:1238	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	13	40	theme	excellent	1219:1227	arg1	cytotoxicity					1195:1206	good cytotoxicity	1190:1206	good cytotoxicity	1190:1206	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	11	41	theme	swelling	1064:1071	arg1	behavior					1073:1080	the swelling behavior	1060:1080	the swelling behavior	1060:1080	The hydroxyapatite offered the inferiority on the swelling behavior.					
31075334	12	42	theme	preliminary	1085:1095	arg1	investigation					1097:1109	A preliminary investigation	1083:1109	A preliminary investigation on the in vitro behavior of Saos-2	1083:1144	A preliminary investigation on the in vitro behavior of Saos-2 was also performed.					
31075334	7	43	theme	hydroxyapatite	703:716	arg1	existence					690:698	The existence	686:698	The existence of hydroxyapatite	686:716	The existence of hydroxyapatite resulted in a higher thermal stability.					
31075334	8	44	theme	force	778:782	arg1	microscopy					784:793	atomic force microscopy	771:793	atomic force microscopy	771:793	Furthermore, atomic force microscopy was used to investigate the roughness of the surface.					
31075334	13	45	theme	engineering	1252:1262	arg1	material					1264:1271	a tissue engineering material	1243:1271	a tissue engineering material	1243:1271	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	13	45	theme	engineering	1252:1262	arg1	properties					1229:1238	excellent properties	1219:1238	excellent properties	1219:1238	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	13	45	theme	engineering	1252:1262	arg1	cytotoxicity					1195:1206	good cytotoxicity	1190:1206	good cytotoxicity	1190:1206	The composite presented good cytotoxicity, and thus, excellent properties as a tissue engineering material.					
31075334	4	46	theme	10-30 wt	403:410	arg1	hydroxyapatite					416:429	hydroxyapatite	416:429	hydroxyapatite	416:429	Then, 10-30 wt% of hydroxyapatite was loaded into the chitosan film.					
31075334	4	46	theme	10-30 wt	403:410	arg1	%					411:411	10-30 wt%	403:411	10-30 wt% of hydroxyapatite	403:429	Then, 10-30 wt% of hydroxyapatite was loaded into the chitosan film.					
31075334	10	47	theme	phosphate	980:988	arg1	solution					1004:1011	phosphate buffer saline solution	980:1011	phosphate buffer saline solution	980:1011	The swelling behavior of the composite was observed in phosphate buffer saline solution.					
31075334	6	48	theme	composite	675:683	arg1	stability					658:666	the thermal stability	646:666	the thermal stability of the composite	646:683	A thermogravimetric analysis showed a significant enhancement in the thermal stability of the composite.					
31075334	1	49	theme	raw	219:221	arg1	waste					208:212	eggshell waste	199:212	eggshell waste	199:212	Hydroxyapatite was successfully synthesized using eggshell waste as a raw material.					
31075334	1	49	theme	raw	219:221	arg1	material					223:230	a raw material	217:230	a raw material	217:230	Hydroxyapatite was successfully synthesized using eggshell waste as a raw material.					
31075334	4	50	theme	hydroxyapatite	416:429	arg1	hydroxyapatite					416:429	hydroxyapatite	416:429	hydroxyapatite	416:429	Then, 10-30 wt% of hydroxyapatite was loaded into the chitosan film.					
31075334	4	50	theme	hydroxyapatite	416:429	arg1	%					411:411	10-30 wt%	403:411	10-30 wt% of hydroxyapatite	403:429	Then, 10-30 wt% of hydroxyapatite was loaded into the chitosan film.					
31075334	2	51	theme	orthophosphoric	252:266	arg1	acid					268:271	orthophosphoric acid	252:271	orthophosphoric acid	252:271	Eggshell waste and orthophosphoric acid were co-precipitated for 2 h at an ambient temperature.					
31075334	5	52	theme	dispersive	506:515	arg1	analyses					517:524	energy dispersive analyses	499:524	energy dispersive analyses	499:524	Scanning electron microscopy and energy dispersive analyses confirmed the morphological properties and dispersion.					
31075334	8	53	theme	surface	840:846	arg1	roughness					823:831	the roughness	819:831	the roughness of the surface	819:846	Furthermore, atomic force microscopy was used to investigate the roughness of the surface.					
31075334	0	54	theme	chitosan-based	56:69	arg1	composite					71:79	a chitosan-based composite	54:79	a chitosan-based composite	54:79	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	0	55	dep	Development	0:10	arg1	behavior					91:98	In vitro behavior	82:98	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.	0:147	Development of hydroxyapatite from eggshell waste and a chitosan-based composite: In vitro behavior of human osteoblast-like cell (Saos-2) cultures.					
31075334	10	56	theme	swelling	929:936	arg1	behavior					938:945	The swelling behavior	925:945	The swelling behavior of the composite	925:962	The swelling behavior of the composite was observed in phosphate buffer saline solution.					
30889648	0	0	theme	oxidized	94:101	arg1	cellulose					115:123	oxidized regenerated cellulose	94:123	oxidized regenerated cellulose	94:123	Polypropylene composite hernia mesh with anti-adhesion layer composed of polycaprolactone and oxidized regenerated cellulose.					
30889648	11	1	theme	ORC	1354:1356	arg1	degradation					1358:1368	ORC degradation	1354:1368	ORC degradation	1354:1368	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	9	2	theme	bio-compatibility	1159:1175	arg1	performances					1177:1188	bio-compatibility performances	1159:1188	bio-compatibility performances	1159:1188	We conducted in vitro cell culture studies to determine bio-compatibility performances.					
30889648	6	3	theme	Oxidized	608:615	arg1	ORC					640:642	ORC	640:642	ORC	640:642	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	6	3	theme	Oxidized	608:615	arg1	cellulose					629:637	Oxidized regenerated cellulose	608:637	Oxidized regenerated cellulose (ORC)	608:643	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	8	4	theme	electro-spinning	992:1007	arg1	micro-particles					1013:1027	electro-spinning ORC micro-particles	992:1027	electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure	992:1100	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	11	5	theme	anti-adhesion	1486:1498	arg1	properties					1500:1509	efficient anti-adhesion properties	1476:1509	efficient anti-adhesion properties	1476:1509	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	1	6	theme	general	165:171	arg1	operations					181:190	the general surgery operations	161:190	the general surgery operations	161:190	Hernia surgeries are at the top of the general surgery operations.					
30889648	3	7	theme	methods	334:340	arg1	methods					334:340	the most preferred methods	315:340	the most preferred methods	315:340	One of the most preferred methods to prevent adhesion is the use of biomaterials.					
30889648	3	7	theme	methods	334:340	arg1	One					308:310	One	308:310	One	308:310	One of the most preferred methods to prevent adhesion is the use of biomaterials.					
30889648	3	7	theme	methods	334:340	arg1	use					369:371	the use	365:371	the use of biomaterials	365:387	One of the most preferred methods to prevent adhesion is the use of biomaterials.					
30889648	11	8	with	coating	1373:1379	arg1	PCL					1386:1388	PCL	1386:1388	PCL	1386:1388	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	0	9	theme	regenerated	103:113	arg1	cellulose					115:123	oxidized regenerated cellulose	94:123	oxidized regenerated cellulose	94:123	Polypropylene composite hernia mesh with anti-adhesion layer composed of polycaprolactone and oxidized regenerated cellulose.					
30889648	8	10	theme	antibacterial	939:951	arg1	mesh					984:987	an antibacterial and anti-adhesive double-sided mesh	936:987	an antibacterial and anti-adhesive double-sided mesh	936:987	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	8	11	theme	mesh	899:902	arg1	structure					904:912	a composite mesh structure	887:912	a composite mesh structure with ORC	887:921	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	10	12	theme	anti-adhesion	1208:1220	arg1	studies					1258:1264	the anti-adhesion and comprehensive bio-compatibility studies	1204:1264	the anti-adhesion and comprehensive bio-compatibility studies	1204:1264	We evaluated the anti-adhesion and comprehensive bio-compatibility studies through in vivo experiments.					
30889648	8	13	from	poly	1034:1037	arg1	mesh					1067:1070	PP mesh	1064:1070	PP mesh	1064:1070	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	3	14	theme	most	319:322	arg1	methods					334:340	the most preferred methods	315:340	the most preferred methods	315:340	One of the most preferred methods to prevent adhesion is the use of biomaterials.					
30889648	8	15	theme	double-sided	971:982	arg1	mesh					984:987	an antibacterial and anti-adhesive double-sided mesh	936:987	an antibacterial and anti-adhesive double-sided mesh	936:987	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	5	16	theme	anti-adhesive	582:594	arg1	properties					596:605	anti-adhesive properties	582:605	anti-adhesive properties	582:605	However, PP meshes do not have anti-adhesive properties.					
30889648	2	17	theme	operations	270:279	arg1	complications					247:259	the worst complications	237:259	the worst complications of these operations	237:279	However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem.					
30889648	1	18	theme	surgery	173:179	arg1	operations					181:190	the general surgery operations	161:190	the general surgery operations	161:190	Hernia surgeries are at the top of the general surgery operations.					
30889648	3	19	theme	preferred	324:332	arg1	methods					334:340	the most preferred methods	315:340	the most preferred methods	315:340	One of the most preferred methods to prevent adhesion is the use of biomaterials.					
30889648	3	20	theme	biomaterials	376:387	arg1	methods					334:340	the most preferred methods	315:340	the most preferred methods	315:340	One of the most preferred methods to prevent adhesion is the use of biomaterials.					
30889648	3	20	theme	biomaterials	376:387	arg1	One					308:310	One	308:310	One	308:310	One of the most preferred methods to prevent adhesion is the use of biomaterials.					
30889648	3	20	theme	biomaterials	376:387	arg1	use					369:371	the use	365:371	the use of biomaterials	365:387	One of the most preferred methods to prevent adhesion is the use of biomaterials.					
30889648	10	21	dep	in	1274:1275	arg1	vivo					1277:1280	vivo	1277:1280	vivo	1277:1280	We evaluated the anti-adhesion and comprehensive bio-compatibility studies through in vivo experiments.					
30889648	7	22	theme	solubility	784:793	arg1	limitations					795:805	solubility limitations	784:805	solubility limitations	784:805	ORC is not easily processable due to solubility limitations; and it must be used externally.					
30889648	1	23	theme	operations	181:190	arg1	top					154:156	the top	150:156	the top of the general surgery operations	150:190	Hernia surgeries are at the top of the general surgery operations.					
30889648	8	24	theme	composite	1082:1090	arg1	structure					1092:1100	a composite structure	1080:1100	a composite structure	1080:1100	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	4	25	theme	clinical	449:456	arg1	applications					458:469	clinical applications	449:469	clinical applications owing to its mechanically robust structure against deformation within the body	449:548	Polypropylene (PP) mesh is frequently preferred product in clinical applications owing to its mechanically robust structure against deformation within the body.					
30889648	6	26	theme	clinical	733:740	arg1	use					742:744	clinical use	733:744	clinical use	733:744	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	4	27	from	product	438:444	arg1	applications					458:469	clinical applications	449:469	clinical applications owing to its mechanically robust structure against deformation within the body	449:548	Polypropylene (PP) mesh is frequently preferred product in clinical applications owing to its mechanically robust structure against deformation within the body.					
30889648	5	28	theme	PP	560:561	arg1	meshes					563:568	PP meshes	560:568	PP meshes	560:568	However, PP meshes do not have anti-adhesive properties.					
30889648	2	29	theme	major	293:297	arg1	adhesion					211:218	visceral adhesion	202:218	visceral adhesion	202:218	However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem.					
30889648	2	29	theme	major	293:297	arg1	problem					299:305	a major problem	291:305	a major problem	291:305	However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem.					
30889648	6	30	theme	other	653:657	arg1	hand					659:662	the other hand	649:662	the other hand	649:662	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	9	31	theme	in	1116:1117	arg1	studies					1138:1144	in vitro cell culture studies	1116:1144	in vitro cell culture studies	1116:1144	We conducted in vitro cell culture studies to determine bio-compatibility performances.					
30889648	8	32	theme	ORC	1009:1011	arg1	micro-particles					1013:1027	electro-spinning ORC micro-particles	992:1027	electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure	992:1100	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	8	33	theme	composite	889:897	arg1	structure					904:912	a composite mesh structure	887:912	a composite mesh structure with ORC	887:921	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	10	34	theme	bio-compatibility	1240:1256	arg1	studies					1258:1264	the anti-adhesion and comprehensive bio-compatibility studies	1204:1264	the anti-adhesion and comprehensive bio-compatibility studies	1204:1264	We evaluated the anti-adhesion and comprehensive bio-compatibility studies through in vivo experiments.					
30889648	0	35	theme	hernia	24:29	arg1	mesh					31:34	hernia mesh	24:34	hernia mesh	24:34	Polypropylene composite hernia mesh with anti-adhesion layer composed of polycaprolactone and oxidized regenerated cellulose.					
30889648	6	36	from	one	668:670	arg1	hand					659:662	the other hand	649:662	the other hand	649:662	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	9	37	theme	cell	1125:1128	arg1	studies					1138:1144	in vitro cell culture studies	1116:1144	in vitro cell culture studies	1116:1144	We conducted in vitro cell culture studies to determine bio-compatibility performances.					
30889648	1	38	theme	Hernia	126:131	arg1	surgeries					133:141	Hernia surgeries	126:141	Hernia surgeries	126:141	Hernia surgeries are at the top of the general surgery operations.					
30889648	10	39	theme	comprehensive	1226:1238	arg1	studies					1258:1264	the anti-adhesion and comprehensive bio-compatibility studies	1204:1264	the anti-adhesion and comprehensive bio-compatibility studies	1204:1264	We evaluated the anti-adhesion and comprehensive bio-compatibility studies through in vivo experiments.					
30889648	6	40	from	adhesion	721:728	arg1	use					742:744	clinical use	733:744	clinical use	733:744	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	0	41	theme	anti-adhesion	41:53	arg1	layer					55:59	anti-adhesion layer	41:59	anti-adhesion layer composed of polycaprolactone and oxidized regenerated cellulose	41:123	Polypropylene composite hernia mesh with anti-adhesion layer composed of polycaprolactone and oxidized regenerated cellulose.					
30889648	9	42	theme	culture	1130:1136	arg1	studies					1138:1144	in vitro cell culture studies	1116:1144	in vitro cell culture studies	1116:1144	We conducted in vitro cell culture studies to determine bio-compatibility performances.					
30889648	11	43	theme	adhesion	1416:1423	arg1	prevention					1425:1434	adhesion prevention	1416:1434	adhesion prevention	1416:1434	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	11	44	theme	efficient	1476:1484	arg1	properties					1500:1509	efficient anti-adhesion properties	1476:1509	efficient anti-adhesion properties	1476:1509	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	8	45	with	structure	904:912	arg1	ORC					919:921	ORC	919:921	ORC	919:921	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	4	46	theme	robust	497:502	arg1	structure					504:512	its mechanically robust structure	480:512	its mechanically robust structure	480:512	Polypropylene (PP) mesh is frequently preferred product in clinical applications owing to its mechanically robust structure against deformation within the body.					
30889648	11	47	theme	important	1398:1406	arg1	role					1408:1411	an important role	1395:1411	an important role	1395:1411	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	11	48	theme	degradation	1358:1368	arg1	presence					1325:1332	ORC presence	1321:1332	ORC presence	1321:1332	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	11	48	theme	degradation	1358:1368	arg1	optimization					1338:1349	optimization	1338:1349	optimization of ORC degradation by coating with PCL	1338:1388	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	10	49	theme	in	1274:1275	arg1	experiments					1282:1292	in vivo experiments	1274:1292	in vivo experiments	1274:1292	We evaluated the anti-adhesion and comprehensive bio-compatibility studies through in vivo experiments.					
30889648	8	50	theme	anti-adhesive	957:969	arg1	mesh					984:987	an antibacterial and anti-adhesive double-sided mesh	936:987	an antibacterial and anti-adhesive double-sided mesh	936:987	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	0	51	with	composite	14:22	arg1	layer					55:59	anti-adhesion layer	41:59	anti-adhesion layer composed of polycaprolactone and oxidized regenerated cellulose	41:123	Polypropylene composite hernia mesh with anti-adhesion layer composed of polycaprolactone and oxidized regenerated cellulose.					
30889648	4	52	theme	Polypropylene	390:402	arg1	mesh					409:412	Polypropylene (PP) mesh	390:412	Polypropylene (PP) mesh	390:412	Polypropylene (PP) mesh is frequently preferred product in clinical applications owing to its mechanically robust structure against deformation within the body.					
30889648	6	53	theme	products	694:701	arg1	products					694:701	the most preferred products	675:701	the most preferred products	675:701	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	6	53	theme	products	694:701	arg1	one					668:670	one	668:670	one	668:670	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	11	54	theme	ORC	1321:1323	arg1	presence					1325:1332	ORC presence	1321:1332	ORC presence	1321:1332	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	8	55	with	micro-particles	1013:1027	arg1	ε‑caprolactone					1039:1052	ε‑caprolactone	1039:1052	ε‑caprolactone	1039:1052	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	8	55	with	micro-particles	1013:1027	arg1	PCL					1056:1058	PCL	1056:1058	PCL	1056:1058	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	8	55	with	micro-particles	1013:1027	arg1	poly					1034:1037	poly	1034:1037	poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure	1034:1100	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	2	56	theme	visceral	202:209	arg1	adhesion					211:218	visceral adhesion	202:218	visceral adhesion	202:218	However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem.					
30889648	2	56	theme	visceral	202:209	arg1	problem					299:305	a major problem	291:305	a major problem	291:305	However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem.					
30889648	2	57	theme	complications	247:259	arg1	one					230:232	one	230:232	one	230:232	However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem.					
30889648	2	57	theme	complications	247:259	arg1	complications					247:259	the worst complications	237:259	the worst complications of these operations	237:279	However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem.					
30889648	6	58	theme	preferred	684:692	arg1	products					694:701	the most preferred products	675:701	the most preferred products	675:701	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	11	59	theme	product	1453:1459	arg1	prototype					1461:1469	a product prototype	1451:1469	a product prototype	1451:1469	The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.					
30889648	4	60	theme	PP	405:406	arg1	mesh					409:412	Polypropylene (PP) mesh	390:412	Polypropylene (PP) mesh	390:412	Polypropylene (PP) mesh is frequently preferred product in clinical applications owing to its mechanically robust structure against deformation within the body.					
30889648	2	61	theme	worst	241:245	arg1	complications					247:259	the worst complications	237:259	the worst complications of these operations	237:279	However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem.					
30889648	8	62	theme	first	863:867	arg1	time					869:872	the first time	859:872	the first time	859:872	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
30889648	5	63	contain	have	577:580	arg2	properties					596:605	anti-adhesive properties	582:605	anti-adhesive properties	582:605	However, PP meshes do not have anti-adhesive properties.					
30889648	5	63	contain	have	577:580	arg1	meshes					563:568	PP meshes	560:568	PP meshes	560:568	However, PP meshes do not have anti-adhesive properties.					
30889648	7	64	used	used	823:826	arg2	it					812:813	it	812:813	it	812:813	ORC is not easily processable due to solubility limitations; and it must be used externally.					
30889648	9	65	dep	in	1116:1117	arg1	vitro					1119:1123	vitro	1119:1123	vitro	1119:1123	We conducted in vitro cell culture studies to determine bio-compatibility performances.					
30889648	6	66	theme	regenerated	617:627	arg1	ORC					640:642	ORC	640:642	ORC	640:642	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	6	66	theme	regenerated	617:627	arg1	cellulose					629:637	Oxidized regenerated cellulose	608:637	Oxidized regenerated cellulose (ORC)	608:643	Oxidized regenerated cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use.					
30889648	8	67	theme	PP	1064:1065	arg1	mesh					1067:1070	PP mesh	1064:1070	PP mesh	1064:1070	In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(ε‑caprolactone) (PCL) on PP mesh to form a composite structure.					
31581359	0	0	theme	effects	118:124	arg1	fabrication					11:21	fabrication	11:21	fabrication	11:21	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	0	0	theme	effects	118:124	arg1	Design					0:5	Design	0:5	Design	0:5	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	0	0	theme	effects	118:124	arg1	evaluation					100:109	evaluation	100:109	evaluation of its effects on bone tissue engineering	100:151	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	0	1	from	fabrication	11:21	arg1	engineering					141:151	bone tissue engineering	129:151	bone tissue engineering	129:151	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	6	2	from	rate	1015:1018	arg1	SBF					1069:1071	SBF	1069:1071	SBF	1069:1071	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	2	from	rate	1015:1018	arg1	solution					1059:1066	simulated body fluid solution	1038:1066	simulated body fluid solution (SBF)	1038:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	1	3	with	clinoptilolite-nanohydroxyapatite/chitosan-gelatin	248:297	arg1	attributes					328:337	enhanced attributes	319:337	enhanced attributes for utilization in the bone tissue engineering	319:384	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	6	4	theme	mechanical	1078:1087	arg1	strength					1089:1096	mechanical strength	1078:1096	mechanical strength	1078:1096	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	5	theme	CLN	930:932	arg1	presence					918:925	the presence	914:925	the presence of CLN and nHA	914:940	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	7	6	theme	CLN-nHA/CS-G	1119:1130	arg1	scaffold					1132:1139	the CLN-nHA/CS-G scaffold	1115:1139	the CLN-nHA/CS-G scaffold	1115:1139	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	0	7	theme	bone	129:132	arg1	engineering					141:151	bone tissue engineering	129:151	bone tissue engineering	129:151	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	0	8	from	Design	0:5	arg1	engineering					141:151	bone tissue engineering	129:151	bone tissue engineering	129:151	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	6	9	from	degradation	1023:1033	arg1	SBF					1069:1071	SBF	1069:1071	SBF	1069:1071	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	9	from	degradation	1023:1033	arg1	solution					1059:1066	simulated body fluid solution	1038:1066	simulated body fluid solution (SBF)	1038:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	10	from	solution	1059:1066	arg1	strength					1089:1096	mechanical strength	1078:1096	mechanical strength	1078:1096	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	10	from	solution	1059:1066	arg1	rate					1015:1018	low rate	1011:1018	low rate of degradation in simulated body fluid solution (SBF)	1011:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	10	from	solution	1059:1066	arg1	area					972:975	an increased surface area	951:975	an increased surface area	951:975	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	10	from	solution	1059:1066	arg1	biomineralization					988:1004	increased biomineralization	978:1004	increased biomineralization	978:1004	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	3	11	theme	XRD	580:582	arg1	techniques					593:602	BET, FTIR, XRD, and SEM techniques	569:602	BET, FTIR, XRD, and SEM techniques	569:602	The fabricated composite scaffolds were studied using BET, FTIR, XRD, and SEM techniques.					
31581359	6	12	from	strength	1089:1096	arg1	SBF					1069:1071	SBF	1069:1071	SBF	1069:1071	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	12	from	strength	1089:1096	arg1	solution					1059:1066	simulated body fluid solution	1038:1066	simulated body fluid solution (SBF)	1038:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	1	13	theme	enhanced	319:326	arg1	attributes					328:337	enhanced attributes	319:337	enhanced attributes for utilization in the bone tissue engineering	319:384	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	7	14	theme	pulp	1182:1185	arg1	h-DPSCs					1199:1205	h-DPSCs	1199:1205	h-DPSCs	1199:1205	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	7	14	theme	pulp	1182:1185	arg1	cells					1192:1196	human dental pulp stem cells	1169:1196	human dental pulp stem cells (h-DPSCs)	1169:1206	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	9	15	theme	bone	1479:1482	arg1	engineering					1491:1501	bone tissue engineering	1479:1501	bone tissue engineering	1479:1501	Results indicated that introducing CLN and nHA to composite improves the scaffold characteristics in a way that makes it suitable for bone tissue engineering.					
31581359	6	16	theme	increased	978:986	arg1	biomineralization					988:1004	increased biomineralization	978:1004	increased biomineralization	978:1004	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	8	17	theme	pore	1314:1317	arg1	surfaces					1319:1326	the pore surfaces	1310:1326	the pore surfaces of the scaffold	1310:1342	The biological response of h-DPSCs showed no toxicity and studied cells proliferated and attached on the pore surfaces of the scaffold.					
31581359	4	18	theme	porous	616:621	arg1	scaffolds					633:641	The highly porous composite scaffolds	605:641	The highly porous composite scaffolds with a pore size of 200 ± 100 μm	605:674	The highly porous composite scaffolds with a pore size of 200 ± 100 μm were synthesized.					
31581359	7	19	theme	dental	1175:1180	arg1	h-DPSCs					1199:1205	h-DPSCs	1199:1205	h-DPSCs	1199:1205	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	7	19	theme	dental	1175:1180	arg1	cells					1192:1196	human dental pulp stem cells	1169:1196	human dental pulp stem cells (h-DPSCs)	1169:1206	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	7	20	from	assay	1160:1164	arg1	h-DPSCs					1199:1205	h-DPSCs	1199:1205	h-DPSCs	1199:1205	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	7	20	from	assay	1160:1164	arg1	cells					1192:1196	human dental pulp stem cells	1169:1196	human dental pulp stem cells (h-DPSCs)	1169:1206	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	6	21	from	biomineralization	988:1004	arg1	SBF					1069:1071	SBF	1069:1071	SBF	1069:1071	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	21	from	biomineralization	988:1004	arg1	solution					1059:1066	simulated body fluid solution	1038:1066	simulated body fluid solution (SBF)	1038:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	22	theme	body	1048:1051	arg1	SBF					1069:1071	SBF	1069:1071	SBF	1069:1071	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	22	theme	body	1048:1051	arg1	solution					1059:1066	simulated body fluid solution	1038:1066	simulated body fluid solution (SBF)	1038:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	3	23	theme	FTIR	574:577	arg1	techniques					593:602	BET, FTIR, XRD, and SEM techniques	569:602	BET, FTIR, XRD, and SEM techniques	569:602	The fabricated composite scaffolds were studied using BET, FTIR, XRD, and SEM techniques.					
31581359	8	24	theme	biological	1213:1222	arg1	response					1224:1231	The biological response	1209:1231	The biological response of h-DPSCs	1209:1242	The biological response of h-DPSCs showed no toxicity and studied cells proliferated and attached on the pore surfaces of the scaffold.					
31581359	0	25	theme	tissue	134:139	arg1	engineering					141:151	bone tissue engineering	129:151	bone tissue engineering	129:151	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	9	26	theme	tissue	1484:1489	arg1	engineering					1491:1501	bone tissue engineering	1479:1501	bone tissue engineering	1479:1501	Results indicated that introducing CLN and nHA to composite improves the scaffold characteristics in a way that makes it suitable for bone tissue engineering.					
31581359	7	27	theme	stem	1187:1190	arg1	h-DPSCs					1199:1205	h-DPSCs	1199:1205	h-DPSCs	1199:1205	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	7	27	theme	stem	1187:1190	arg1	cells					1192:1196	human dental pulp stem cells	1169:1196	human dental pulp stem cells (h-DPSCs)	1169:1206	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	2	28	theme	nHA	445:447	arg1	solution					472:479	the CLN, nHA, chitosan, and gelatin solution	436:479	the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step	436:512	This composite scaffold was prepared by blending the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step.					
31581359	6	29	theme	CS-G	899:902	arg1	scaffold					904:911	CS-G scaffold	899:911	CS-G scaffold	899:911	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	1	30	theme	innovative	197:206	arg1	scaffold					218:225	an innovative composite scaffold	194:225	an innovative composite scaffold	194:225	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	7	31	theme	human	1169:1173	arg1	h-DPSCs					1199:1205	h-DPSCs	1199:1205	h-DPSCs	1199:1205	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	7	31	theme	human	1169:1173	arg1	cells					1192:1196	human dental pulp stem cells	1169:1196	human dental pulp stem cells (h-DPSCs)	1169:1206	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	1	32	from	utilization	343:353	arg1	engineering					374:384	the bone tissue engineering	358:384	the bone tissue engineering	358:384	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	8	33	theme	scaffold	1335:1342	arg1	surfaces					1319:1326	the pore surfaces	1310:1326	the pore surfaces of the scaffold	1310:1342	The biological response of h-DPSCs showed no toxicity and studied cells proliferated and attached on the pore surfaces of the scaffold.					
31581359	5	34	theme	physicochemical	738:752	arg1	ratio					805:809	swelling ratio	796:809	swelling ratio	796:809	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	34	theme	physicochemical	738:752	arg1	behavior					862:869	mechanical behavior	851:869	mechanical behavior	851:869	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	34	theme	physicochemical	738:752	arg1	biomineralization					812:828	biomineralization	812:828	biomineralization	812:828	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	34	theme	physicochemical	738:752	arg1	density					787:793	density	787:793	density	787:793	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	34	theme	physicochemical	738:752	arg1	biodegradation					831:844	biodegradation	831:844	biodegradation	831:844	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	34	theme	physicochemical	738:752	arg1	features					754:761	the physicochemical features	734:761	the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior	734:869	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	1	35	theme	composite	208:216	arg1	scaffold					218:225	an innovative composite scaffold	194:225	an innovative composite scaffold	194:225	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	7	36	theme	MTT	1156:1158	arg1	assay					1160:1164	MTT assay	1156:1164	MTT assay on human dental pulp stem cells (h-DPSCs)	1156:1206	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	2	37	theme	gelatin	464:470	arg1	solution					472:479	the CLN, nHA, chitosan, and gelatin solution	436:479	the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step	436:512	This composite scaffold was prepared by blending the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step.					
31581359	6	38	theme	surface	964:970	arg1	area					972:975	an increased surface area	951:975	an increased surface area	951:975	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	5	39	theme	nHA	727:729	arg1	effects					708:714	the effects	704:714	the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior	704:869	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	0	40	from	effects	118:124	arg1	engineering					141:151	bone tissue engineering	129:151	bone tissue engineering	129:151	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	3	41	theme	SEM	589:591	arg1	techniques					593:602	BET, FTIR, XRD, and SEM techniques	569:602	BET, FTIR, XRD, and SEM techniques	569:602	The fabricated composite scaffolds were studied using BET, FTIR, XRD, and SEM techniques.					
31581359	5	42	theme	mechanical	851:860	arg1	behavior					862:869	mechanical behavior	851:869	mechanical behavior	851:869	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	0	43	theme	composite	77:85	arg1	scaffold					87:94	clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold	26:94	clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold	26:94	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	6	44	theme	increased	954:962	arg1	area					972:975	an increased surface area	951:975	an increased surface area	951:975	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	8	45	theme	h-DPSCs	1236:1242	arg1	response					1224:1231	The biological response	1209:1231	The biological response of h-DPSCs	1209:1242	The biological response of h-DPSCs showed no toxicity and studied cells proliferated and attached on the pore surfaces of the scaffold.					
31581359	2	46	theme	CLN	440:442	arg1	solution					472:479	the CLN, nHA, chitosan, and gelatin solution	436:479	the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step	436:512	This composite scaffold was prepared by blending the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step.					
31581359	6	47	theme	simulated	1038:1046	arg1	SBF					1069:1071	SBF	1069:1071	SBF	1069:1071	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	47	theme	simulated	1038:1046	arg1	solution					1059:1066	simulated body fluid solution	1038:1066	simulated body fluid solution (SBF)	1038:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	0	48	theme	clinoptilolite-nanohydroxyapatite/chitosan-gelatin	26:75	arg1	scaffold					87:94	clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold	26:94	clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold	26:94	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	1	49	theme	bone	362:365	arg1	engineering					374:384	the bone tissue engineering	358:384	the bone tissue engineering	358:384	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	5	50	theme	swelling	796:803	arg1	ratio					805:809	swelling ratio	796:809	swelling ratio	796:809	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	6	51	from	area	972:975	arg1	SBF					1069:1071	SBF	1069:1071	SBF	1069:1071	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	51	from	area	972:975	arg1	solution					1059:1066	simulated body fluid solution	1038:1066	simulated body fluid solution (SBF)	1038:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	2	52	theme	chitosan	450:457	arg1	solution					472:479	the CLN, nHA, chitosan, and gelatin solution	436:479	the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step	436:512	This composite scaffold was prepared by blending the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step.					
31581359	7	53	theme	scaffold	1132:1139	arg1	Cytotoxicity					1099:1110	Cytotoxicity	1099:1110	Cytotoxicity of the CLN-nHA/CS-G scaffold	1099:1139	Cytotoxicity of the CLN-nHA/CS-G scaffold was studied by MTT assay on human dental pulp stem cells (h-DPSCs).					
31581359	1	54	theme	tissue	367:372	arg1	engineering					374:384	the bone tissue engineering	358:384	the bone tissue engineering	358:384	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	0	55	from	evaluation	100:109	arg1	engineering					141:151	bone tissue engineering	129:151	bone tissue engineering	129:151	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	0	56	theme	scaffold	87:94	arg1	fabrication					11:21	fabrication	11:21	fabrication	11:21	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	0	56	theme	scaffold	87:94	arg1	Design					0:5	Design	0:5	Design	0:5	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	0	56	theme	scaffold	87:94	arg1	evaluation					100:109	evaluation	100:109	evaluation of its effects on bone tissue engineering	100:151	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	3	57	theme	fabricated	519:528	arg1	scaffolds					540:548	The fabricated composite scaffolds	515:548	The fabricated composite scaffolds	515:548	The fabricated composite scaffolds were studied using BET, FTIR, XRD, and SEM techniques.					
31581359	0	58	from	engineering	141:151	arg1	fabrication					11:21	fabrication	11:21	fabrication	11:21	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	0	58	from	engineering	141:151	arg1	Design					0:5	Design	0:5	Design	0:5	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	0	58	from	engineering	141:151	arg1	evaluation					100:109	evaluation	100:109	evaluation of its effects on bone tissue engineering	100:151	Design and fabrication of clinoptilolite-nanohydroxyapatite/chitosan-gelatin composite scaffold and evaluation of its effects on bone tissue engineering.					
31581359	4	59	with	scaffolds	633:641	arg1	size					655:658	a pore size	648:658	a pore size of 200 ± 100 μm	648:674	The highly porous composite scaffolds with a pore size of 200 ± 100 μm were synthesized.					
31581359	6	60	theme	nHA	938:940	arg1	presence					918:925	the presence	914:925	the presence of CLN and nHA	914:940	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	2	61	theme	composite	392:400	arg1	scaffold					402:409	This composite scaffold	387:409	This composite scaffold	387:409	This composite scaffold was prepared by blending the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step.					
31581359	6	62	theme	degradation	1023:1033	arg1	strength					1089:1096	mechanical strength	1078:1096	mechanical strength	1078:1096	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	62	theme	degradation	1023:1033	arg1	rate					1015:1018	low rate	1011:1018	low rate of degradation in simulated body fluid solution (SBF)	1011:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	62	theme	degradation	1023:1033	arg1	area					972:975	an increased surface area	951:975	an increased surface area	951:975	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	62	theme	degradation	1023:1033	arg1	biomineralization					988:1004	increased biomineralization	978:1004	increased biomineralization	978:1004	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	5	63	theme	CLN	719:721	arg1	effects					708:714	the effects	704:714	the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior	704:869	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	8	64	dep	cells	1275:1279	arg1	attached					1298:1305	attached	1298:1305	attached on the pore surfaces of the scaffold	1298:1342	The biological response of h-DPSCs showed no toxicity and studied cells proliferated and attached on the pore surfaces of the scaffold.					
31581359	8	64	dep	cells	1275:1279	arg1	proliferated					1281:1292	proliferated	1281:1292	proliferated	1281:1292	The biological response of h-DPSCs showed no toxicity and studied cells proliferated and attached on the pore surfaces of the scaffold.					
31581359	1	65	dep	structured	234:243	arg1	scaffold					218:225	an innovative composite scaffold	194:225	an innovative composite scaffold	194:225	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	9	66	from	characteristics	1427:1441	arg1	way					1448:1450	a way	1446:1450	a way that makes it suitable for bone tissue engineering	1446:1501	Results indicated that introducing CLN and nHA to composite improves the scaffold characteristics in a way that makes it suitable for bone tissue engineering.					
31581359	3	67	theme	composite	530:538	arg1	scaffolds					540:548	The fabricated composite scaffolds	515:548	The fabricated composite scaffolds	515:548	The fabricated composite scaffolds were studied using BET, FTIR, XRD, and SEM techniques.					
31581359	2	68	theme	freeze-drying	495:507	arg1	step					509:512	a freeze-drying step	493:512	a freeze-drying step	493:512	This composite scaffold was prepared by blending the CLN, nHA, chitosan, and gelatin solution followed by a freeze-drying step.					
31581359	1	69	theme	clinoptilolite-nanohydroxyapatite/chitosan-gelatin	248:297	arg1	structured					234:243	structured	234:243	structured	234:243	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	5	70	theme	scaffold	770:777	arg1	ratio					805:809	swelling ratio	796:809	swelling ratio	796:809	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	70	theme	scaffold	770:777	arg1	behavior					862:869	mechanical behavior	851:869	mechanical behavior	851:869	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	70	theme	scaffold	770:777	arg1	biomineralization					812:828	biomineralization	812:828	biomineralization	812:828	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	70	theme	scaffold	770:777	arg1	density					787:793	density	787:793	density	787:793	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	70	theme	scaffold	770:777	arg1	biodegradation					831:844	biodegradation	831:844	biodegradation	831:844	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	70	theme	scaffold	770:777	arg1	features					754:761	the physicochemical features	734:761	the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior	734:869	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	4	71	theme	200 ± 100 μm	663:674	arg1	size					655:658	a pore size	648:658	a pore size of 200 ± 100 μm	648:674	The highly porous composite scaffolds with a pore size of 200 ± 100 μm were synthesized.					
31581359	6	72	theme	fluid	1053:1057	arg1	SBF					1069:1071	SBF	1069:1071	SBF	1069:1071	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	6	72	theme	fluid	1053:1057	arg1	solution					1059:1066	simulated body fluid solution	1038:1066	simulated body fluid solution (SBF)	1038:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	3	73	theme	BET	569:571	arg1	techniques					593:602	BET, FTIR, XRD, and SEM techniques	569:602	BET, FTIR, XRD, and SEM techniques	569:602	The fabricated composite scaffolds were studied using BET, FTIR, XRD, and SEM techniques.					
31581359	9	74	theme	scaffold	1418:1425	arg1	characteristics					1427:1441	the scaffold characteristics	1414:1441	the scaffold characteristics in a way that makes it suitable for bone tissue engineering	1414:1501	Results indicated that introducing CLN and nHA to composite improves the scaffold characteristics in a way that makes it suitable for bone tissue engineering.					
31581359	4	75	theme	pore	650:653	arg1	size					655:658	a pore size	648:658	a pore size of 200 ± 100 μm	648:674	The highly porous composite scaffolds with a pore size of 200 ± 100 μm were synthesized.					
31581359	4	76	theme	composite	623:631	arg1	scaffolds					633:641	The highly porous composite scaffolds	605:641	The highly porous composite scaffolds with a pore size of 200 ± 100 μm	605:674	The highly porous composite scaffolds with a pore size of 200 ± 100 μm were synthesized.					
31581359	6	77	theme	low	1011:1013	arg1	rate					1015:1018	low rate	1011:1018	low rate of degradation in simulated body fluid solution (SBF)	1011:1072	Compared with CS-G scaffold, the presence of CLN and nHA leads to an increased surface area, increased biomineralization, and low rate of degradation in simulated body fluid solution (SBF) and mechanical strength.					
31581359	1	78	theme	study	170:174	arg1	aim					158:160	The aim	154:160	The aim of this study	154:174	The aim of this study was to synthesize an innovative composite scaffold, which structured of clinoptilolite-nanohydroxyapatite/chitosan-gelatin (CLN-nHA/CS-G) with enhanced attributes for utilization in the bone tissue engineering.					
31581359	5	79	from	effects	708:714	arg1	ratio					805:809	swelling ratio	796:809	swelling ratio	796:809	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	79	from	effects	708:714	arg1	behavior					862:869	mechanical behavior	851:869	mechanical behavior	851:869	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	79	from	effects	708:714	arg1	biomineralization					812:828	biomineralization	812:828	biomineralization	812:828	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	79	from	effects	708:714	arg1	density					787:793	density	787:793	density	787:793	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	79	from	effects	708:714	arg1	biodegradation					831:844	biodegradation	831:844	biodegradation	831:844	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31581359	5	79	from	effects	708:714	arg1	features					754:761	the physicochemical features	734:761	the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior	734:869	Moreover, the effects of CLN and nHA on the physicochemical features of the scaffold such as density, swelling ratio, biomineralization, biodegradation, and mechanical behavior were studied.					
31629214	7	0	theme	diseases	1194:1201	arg1	diagnosis					1163:1171	early diagnosis	1157:1171	early diagnosis of neurodegenerative diseases	1157:1201	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	7	1	theme	resorbable	1038:1047	arg1	biostrip					1049:1056	The presented resorbable biostrip	1024:1056	The presented resorbable biostrip	1024:1056	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	2	2	dep	graphene-based	310:323	arg1	interconnects					325:337	interconnects	325:337	interconnects	325:337	The biostrip consists of mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode and graphene-based interconnects.					
31629214	3	3	theme	pore	405:408	arg1	size					410:413	average pore size	397:413	average pore size of 1μmwith slow (2 h) curing process	397:450	Obtained with particulate leaching, the m-chit-GO showed average pore size of 1μmwith slow (2 h) curing process.					
31629214	6	4	theme	bulk	997:1000	arg1	state					1002:1006	their bulk state	991:1006	their bulk state (100 nM) on DA	991:1021	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement revealed significantly higher response for m-Chit-GO (10pM) as compared to their bulk state (100 nM) on DA.					
31629214	6	4	theme	bulk	997:1000	arg1	100 nM					1009:1014	100 nM	1009:1014	100 nM	1009:1014	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement revealed significantly higher response for m-Chit-GO (10pM) as compared to their bulk state (100 nM) on DA.					
31629214	5	5	theme	working	715:721	arg1	electrode					723:731	working electrode	715:731	working electrode	715:731	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	6	6	theme	enhancement	904:914	arg1	analysis					842:849	The quantitative analysis	825:849	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement	825:914	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement revealed significantly higher response for m-Chit-GO (10pM) as compared to their bulk state (100 nM) on DA.					
31629214	5	7	dep	electrodes	799:808	arg1	counter					791:797	counter	791:797	counter	791:797	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	7	8	theme	neurodegenerative	1176:1192	arg1	diseases					1194:1201	neurodegenerative diseases	1176:1201	neurodegenerative diseases	1176:1201	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	6	9	theme	peak-current	891:902	arg1	enhancement					904:914	concentration-dependent voltammetric peak-current enhancement	854:914	concentration-dependent voltammetric peak-current enhancement	854:914	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement revealed significantly higher response for m-Chit-GO (10pM) as compared to their bulk state (100 nM) on DA.					
31629214	5	10	theme	three-electrode	663:677	arg1	set-up					679:684	three-electrode set-up	663:684	three-electrode set-up	663:684	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	6	11	theme	voltammetric	878:889	arg1	enhancement					904:914	concentration-dependent voltammetric peak-current enhancement	854:914	concentration-dependent voltammetric peak-current enhancement	854:914	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement revealed significantly higher response for m-Chit-GO (10pM) as compared to their bulk state (100 nM) on DA.					
31629214	5	12	used	used	767:770	arg2	reference					777:785	reference	777:785	reference	777:785	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	5	12	used	used	767:770	arg2	electrodes					799:808	counter electrodes	791:808	counter electrodes	791:808	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	5	12	used	used	767:770	arg2	Graphene					753:760	Graphene	753:760	Graphene	753:760	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	5	12	used	used	767:770	arg2	Ag/AgCl					741:747	Ag/AgCl	741:747	Ag/AgCl	741:747	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	1	13	theme	dopamine	186:193	arg1	detection					173:181	label-free electrochemical detection	146:181	label-free electrochemical detection of dopamine (DA)	146:198	This work presents a chitosan based resorbable biostrip for label-free electrochemical detection of dopamine (DA).					
31629214	3	14	theme	average	397:403	arg1	size					410:413	average pore size	397:413	average pore size of 1μmwith slow (2 h) curing process	397:450	Obtained with particulate leaching, the m-chit-GO showed average pore size of 1μmwith slow (2 h) curing process.					
31629214	0	15	theme	resorbable	42:51	arg1	biostrip					53:60	resorbable biostrip	42:60	resorbable biostrip	42:60	Mesoporous chitosan based conformable and resorbable biostrip for dopamine detection.					
31629214	6	16	theme	concentration-dependent	854:876	arg1	enhancement					904:914	concentration-dependent voltammetric peak-current enhancement	854:914	concentration-dependent voltammetric peak-current enhancement	854:914	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement revealed significantly higher response for m-Chit-GO (10pM) as compared to their bulk state (100 nM) on DA.					
31629214	3	17	theme	slow	426:429	arg1	process					444:450	1μmwith slow (2 h) curing process	418:450	1μmwith slow (2 h) curing process	418:450	Obtained with particulate leaching, the m-chit-GO showed average pore size of 1μmwith slow (2 h) curing process.					
31629214	7	18	theme	levels	1146:1151	arg1	detection					1130:1138	detection	1130:1138	detection of DA levels	1130:1151	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	4	19	from	response	457:464	arg1	m-Chit-GO					475:483	m-Chit-GO	475:483	m-Chit-GO	475:483	The response of DA on m-Chit-GO was investigated and compared with their bulk counterpart to study the effect of mesoporosity on voltammogram output signals.					
31629214	0	20	theme	Mesoporous	0:9	arg1	chitosan					11:18	Mesoporous chitosan	0:18	Mesoporous chitosan	0:18	Mesoporous chitosan based conformable and resorbable biostrip for dopamine detection.					
31629214	5	21	theme	m-Chit-GO	692:700	arg1	electrode					702:710	m-Chit-GO electrode	692:710	m-Chit-GO electrode	692:710	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	7	22	theme	early	1157:1161	arg1	diagnosis					1163:1171	early diagnosis	1157:1171	early diagnosis of neurodegenerative diseases	1157:1201	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	4	23	theme	mesoporosity	566:577	arg1	effect					556:561	the effect	552:561	the effect of mesoporosity on voltammogram output signals	552:608	The response of DA on m-Chit-GO was investigated and compared with their bulk counterpart to study the effect of mesoporosity on voltammogram output signals.					
31629214	5	24	theme	voltammetric	615:626	arg1	investigations					628:641	The voltammetric investigations	611:641	The voltammetric investigations	611:641	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	3	25	theme	1μmwith	418:424	arg1	process					444:450	1μmwith slow (2 h) curing process	418:450	1μmwith slow (2 h) curing process	418:450	Obtained with particulate leaching, the m-chit-GO showed average pore size of 1μmwith slow (2 h) curing process.					
31629214	4	26	theme	output	595:600	arg1	signals					602:608	voltammogram output signals	582:608	voltammogram output signals	582:608	The response of DA on m-Chit-GO was investigated and compared with their bulk counterpart to study the effect of mesoporosity on voltammogram output signals.					
31629214	7	27	theme	DA	1143:1144	arg1	levels					1146:1151	DA levels	1143:1151	DA levels	1143:1151	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	1	28	theme	chitosan	107:114	arg1	biostrip					133:140	a chitosan based resorbable biostrip	105:140	a chitosan based resorbable biostrip for label-free electrochemical detection of dopamine (DA)	105:198	This work presents a chitosan based resorbable biostrip for label-free electrochemical detection of dopamine (DA).					
31629214	7	29	theme	presented	1028:1036	arg1	biostrip					1049:1056	The presented resorbable biostrip	1024:1056	The presented resorbable biostrip	1024:1056	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	3	30	theme	curing	437:442	arg1	process					444:450	1μmwith slow (2 h) curing process	418:450	1μmwith slow (2 h) curing process	418:450	Obtained with particulate leaching, the m-chit-GO showed average pore size of 1μmwith slow (2 h) curing process.					
31629214	4	31	theme	voltammogram	582:593	arg1	signals					602:608	voltammogram output signals	582:608	voltammogram output signals	582:608	The response of DA on m-Chit-GO was investigated and compared with their bulk counterpart to study the effect of mesoporosity on voltammogram output signals.					
31629214	4	32	theme	DA	469:470	arg1	response					457:464	The response	453:464	The response of DA on m-Chit-GO	453:483	The response of DA on m-Chit-GO was investigated and compared with their bulk counterpart to study the effect of mesoporosity on voltammogram output signals.					
31629214	3	33	theme	process	444:450	arg1	size					410:413	average pore size	397:413	average pore size of 1μmwith slow (2 h) curing process	397:450	Obtained with particulate leaching, the m-chit-GO showed average pore size of 1μmwith slow (2 h) curing process.					
31629214	4	34	from	effect	556:561	arg1	signals					602:608	voltammogram output signals	582:608	voltammogram output signals	582:608	The response of DA on m-Chit-GO was investigated and compared with their bulk counterpart to study the effect of mesoporosity on voltammogram output signals.					
31629214	2	35	theme	oxide	254:258	arg1	electrode					296:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	The biostrip consists of mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode and graphene-based interconnects.					
31629214	7	36	theme	10pM	1089:1092	arg1	detection					1076:1084	detection	1076:1084	detection of 10pM	1076:1092	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	3	37	theme	2 h	432:434	arg1	process					444:450	1μmwith slow (2 h) curing process	418:450	1μmwith slow (2 h) curing process	418:450	Obtained with particulate leaching, the m-chit-GO showed average pore size of 1μmwith slow (2 h) curing process.					
31629214	7	38	theme	great	1112:1116	arg1	promise					1118:1124	great promise	1112:1124	great promise for detection of DA levels for early diagnosis of neurodegenerative diseases	1112:1201	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	2	39	theme	mesoporous-chitosan-graphene	225:252	arg1	electrode					296:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	The biostrip consists of mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode and graphene-based interconnects.					
31629214	1	40	theme	based	116:120	arg1	biostrip					133:140	a chitosan based resorbable biostrip	105:140	a chitosan based resorbable biostrip for label-free electrochemical detection of dopamine (DA)	105:198	This work presents a chitosan based resorbable biostrip for label-free electrochemical detection of dopamine (DA).					
31629214	3	41	theme	particulate	354:364	arg1	leaching					366:373	particulate leaching	354:373	particulate leaching	354:373	Obtained with particulate leaching, the m-chit-GO showed average pore size of 1μmwith slow (2 h) curing process.					
31629214	7	42	theme	detection	1076:1084	arg1	limit					1067:1071	a limit	1065:1071	a limit of detection of 10pM	1065:1092	The presented resorbable biostrip offers a limit of detection of 10pM and thereby shows great promise for detection of DA levels for early diagnosis of neurodegenerative diseases.					
31629214	1	43	theme	resorbable	122:131	arg1	biostrip					133:140	a chitosan based resorbable biostrip	105:140	a chitosan based resorbable biostrip for label-free electrochemical detection of dopamine (DA)	105:198	This work presents a chitosan based resorbable biostrip for label-free electrochemical detection of dopamine (DA).					
31629214	6	44	theme	quantitative	829:840	arg1	analysis					842:849	The quantitative analysis	825:849	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement	825:914	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement revealed significantly higher response for m-Chit-GO (10pM) as compared to their bulk state (100 nM) on DA.					
31629214	5	45	dep	reference	777:785	arg1	a					775:775	a	775:775	a	775:775	The voltammetric investigations were performed with three-electrode set-up using m-Chit-GO electrode as working electrode whereas Ag/AgCl and Graphene were used as a reference and counter electrodes, respectively.					
31629214	2	46	theme	sensing	288:294	arg1	electrode					296:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	The biostrip consists of mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode and graphene-based interconnects.					
31629214	6	47	theme	higher	939:944	arg1	response					946:953	significantly higher response	925:953	significantly higher response for m-Chit-GO (10pM)	925:974	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement revealed significantly higher response for m-Chit-GO (10pM) as compared to their bulk state (100 nM) on DA.					
31629214	2	48	theme	composite-based	272:286	arg1	electrode					296:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	The biostrip consists of mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode and graphene-based interconnects.					
31629214	6	49	from	state	1002:1006	arg1	DA					1020:1021	DA	1020:1021	DA	1020:1021	The quantitative analysis of concentration-dependent voltammetric peak-current enhancement revealed significantly higher response for m-Chit-GO (10pM) as compared to their bulk state (100 nM) on DA.					
31629214	1	50	theme	label-free	146:155	arg1	detection					173:181	label-free electrochemical detection	146:181	label-free electrochemical detection of dopamine (DA)	146:198	This work presents a chitosan based resorbable biostrip for label-free electrochemical detection of dopamine (DA).					
31629214	0	51	theme	dopamine	66:73	arg1	detection					75:83	dopamine detection	66:83	dopamine detection	66:83	Mesoporous chitosan based conformable and resorbable biostrip for dopamine detection.					
31629214	4	52	theme	bulk	526:529	arg1	counterpart					531:541	their bulk counterpart	520:541	their bulk counterpart	520:541	The response of DA on m-Chit-GO was investigated and compared with their bulk counterpart to study the effect of mesoporosity on voltammogram output signals.					
31629214	2	53	theme	m-Chit-GO	261:269	arg1	electrode					296:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode	225:304	The biostrip consists of mesoporous-chitosan-graphene oxide (m-Chit-GO) composite-based sensing electrode and graphene-based interconnects.					
31629214	1	54	theme	electrochemical	157:171	arg1	detection					173:181	label-free electrochemical detection	146:181	label-free electrochemical detection of dopamine (DA)	146:198	This work presents a chitosan based resorbable biostrip for label-free electrochemical detection of dopamine (DA).					
31945552	6	0	theme	higher	1136:1141	arg1	activity					1160:1167	higher electrocatalytic activity	1136:1167	higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2	1136:1222	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	3	1	theme	ultrasensitive	711:724	arg1	detection					742:750	the selective and ultrasensitive electrochemical detection	693:750	the selective and ultrasensitive electrochemical detection of H2O2	693:758	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	2	theme	hemin	564:568	arg1	bio-composites					570:583	The stable hemin bio-composites	553:583	The stable hemin bio-composites	553:583	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	7	3	theme	lower	1476:1480	arg1	limit					1492:1496	a lower detection limit	1474:1496	a lower detection limit of 4.0 nM	1474:1506	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	3	4	theme	selective	697:705	arg1	detection					742:750	the selective and ultrasensitive electrochemical detection	693:750	the selective and ultrasensitive electrochemical detection of H2O2	693:758	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	1	5	theme	oxide	186:190	arg1	nanocomposites					207:220	the metal oxide and biopolymer nanocomposites	176:220	the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT)	176:260	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	3	6	theme	H2O2	755:758	arg1	detection					742:750	the selective and ultrasensitive electrochemical detection	693:750	the selective and ultrasensitive electrochemical detection of H2O2	693:758	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	1	7	theme	biopolymer	196:205	arg1	nanocomposites					207:220	the metal oxide and biopolymer nanocomposites	176:220	the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT)	176:260	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	7	8	theme	H2O2	1440:1443	arg1	concentration					1423:1435	the linear concentration	1412:1435	the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM	1412:1506	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	3	9	theme	sonication	619:628	arg1	method					668:673	the bath sonication (37 kHz of frequency, 150 W of power) method	610:673	the bath sonication (37 kHz of frequency, 150 W of power) method	610:673	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	1	10	theme	carbon	237:242	arg1	MWCNT					255:259	MWCNT	255:259	MWCNT	255:259	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	1	10	theme	carbon	237:242	arg1	nanotubes					244:252	multiwalled carbon nanotubes	225:252	multiwalled carbon nanotubes (MWCNT)	225:260	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	1	11	from	nanocomposites	207:220	arg1	MWCNT					255:259	MWCNT	255:259	MWCNT	255:259	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	1	11	from	nanocomposites	207:220	arg1	nanotubes					244:252	multiwalled carbon nanotubes	225:252	multiwalled carbon nanotubes (MWCNT)	225:260	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	6	12	theme	hemin	1256:1260	arg1	electrodes					1271:1280	other investigated hemin modified electrodes	1237:1280	other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO	1237:1320	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	12	theme	hemin	1256:1260	arg1	hemin/CNC-ZnO					1308:1320	hemin/CNC-ZnO	1308:1320	hemin/CNC-ZnO	1308:1320	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	12	theme	hemin	1256:1260	arg1	hemin/MWCNT					1292:1302	hemin/MWCNT	1292:1302	hemin/MWCNT	1292:1302	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	7	13	theme	linear	1416:1421	arg1	concentration					1423:1435	the linear concentration	1412:1435	the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM	1412:1506	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	3	14	theme	frequency	641:649	arg1	37 kHz					631:636	37 kHz	631:636	37 kHz	631:636	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	14	theme	frequency	641:649	arg1	power					661:665	power	661:665	power	661:665	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	14	theme	frequency	641:649	arg1	150 W					652:656	150 W	652:656	150 W	652:656	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	14	theme	frequency	641:649	arg1	frequency					641:649	frequency	641:649	frequency	641:649	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	6	15	theme	other	1237:1241	arg1	electrodes					1271:1280	other investigated hemin modified electrodes	1237:1280	other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO	1237:1320	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	15	theme	other	1237:1241	arg1	hemin/CNC-ZnO					1308:1320	hemin/CNC-ZnO	1308:1320	hemin/CNC-ZnO	1308:1320	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	15	theme	other	1237:1241	arg1	hemin/MWCNT					1292:1302	hemin/MWCNT	1292:1302	hemin/MWCNT	1292:1302	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	0	16	theme	hemin-mediated	54:67	arg1	nanotubes-cellulose					88:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	7	17	dep	4183.3 µM	1459:1467	arg1	up					1453:1454	up	1453:1454	up	1453:1454	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	0	18	theme	carbon	81:86	arg1	nanotubes-cellulose					88:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	5	19	theme	electrochemical	926:940	arg1	behaviour					942:950	an enhanced redox electrochemical behaviour	908:950	an enhanced redox electrochemical behaviour of hemin	908:959	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	4	20	theme	UV-Vis	765:770	arg1	studies					785:791	The UV-Vis spectroscopy studies	761:791	The UV-Vis spectroscopy studies	761:791	The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite.					
31945552	4	21	attach	presence	807:814	arg2	hemin					826:830	native hemin	819:830	native hemin	819:830	The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite.					
31945552	4	21	attach	presence	807:814	arg1	nanocomposite					852:864	MWCNT-CNC/ZnO NR nanocomposite	835:864	MWCNT-CNC/ZnO NR nanocomposite	835:864	The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite.					
31945552	5	22	theme	NR	1009:1010	arg1	nanocomposite					1012:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite	977:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes	977:1069	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	0	23	theme	ultra-sensitive	126:140	arg1	biosensing					142:151	ultra-sensitive biosensing	126:151	ultra-sensitive biosensing of H2O2	126:159	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	2	24	theme	probe	378:382	arg1	sonication					384:393	probe sonication	378:393	probe sonication (frequency = 20 kHz, amplitude = 50) method	378:437	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	0	25	theme	Sonochemical	0:11	arg1	synthesis					13:21	Sonochemical synthesis	0:21	Sonochemical synthesis	0:21	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	1	26	theme	simple	284:289	arg1	method					304:309	a simple sonochemical method	282:309	a simple sonochemical method	282:309	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	6	27	theme	modified	1105:1112	arg1	SPCE					1114:1117	the MWCNT-CNC/ZnO NR/hemin modified SPCE	1078:1117	the MWCNT-CNC/ZnO NR/hemin modified SPCE	1078:1117	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	28	dep	2.3	1126:1128	arg1	folds					1130:1134	folds	1130:1134	folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO	1130:1320	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	5	29	theme	immobilised	983:993	arg1	nanocomposite					1012:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite	977:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes	977:1069	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	6	30	theme	MWCNT-CNC/ZnO	1082:1094	arg1	SPCE					1114:1117	the MWCNT-CNC/ZnO NR/hemin modified SPCE	1078:1117	the MWCNT-CNC/ZnO NR/hemin modified SPCE	1078:1117	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	2	31	theme	MWCNT-cellulose	492:506	arg1	MWCNT-CNC					522:530	MWCNT-CNC	522:530	MWCNT-CNC	522:530	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	2	31	theme	MWCNT-cellulose	492:506	arg1	nanocrystals					508:519	ultrasonically functionalized MWCNT-cellulose nanocrystals	462:519	ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time	462:550	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	3	32	theme	power	661:665	arg1	37 kHz					631:636	37 kHz	631:636	37 kHz	631:636	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	32	theme	power	661:665	arg1	power					661:665	power	661:665	power	661:665	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	32	theme	power	661:665	arg1	150 W					652:656	150 W	652:656	150 W	652:656	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	32	theme	power	661:665	arg1	frequency					641:649	frequency	641:649	frequency	641:649	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	4	33	theme	hemin	826:830	arg1	presence					807:814	the presence	803:814	the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite	803:864	The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite.					
31945552	1	34	theme	metal	180:184	arg1	oxide					186:190	metal oxide	180:190	metal oxide	180:190	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	7	35	theme	amperometric	1367:1378	arg1	response					1380:1387	a stable amperometric response	1358:1387	a stable amperometric response (-0.2 V vs Ag/AgCl)	1358:1407	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	4	36	theme	MWCNT-CNC/ZnO	835:847	arg1	nanocomposite					852:864	MWCNT-CNC/ZnO NR nanocomposite	835:864	MWCNT-CNC/ZnO NR nanocomposite	835:864	The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite.					
31945552	6	37	with	activity	1160:1167	arg1	-0.2 V					1203:1208	-0.2 V	1203:1208	-0.2 V	1203:1208	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	37	with	activity	1160:1167	arg1	potential					1192:1200	a lower reduction potential	1174:1200	a lower reduction potential (-0.2 V)	1174:1209	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	2	38	theme	ZnO	350:352	arg1	oxide					343:347	zinc oxide	338:347	zinc oxide (ZnO NR)	338:356	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	2	38	theme	ZnO	350:352	arg1	NR					354:355	ZnO NR	350:355	ZnO NR	350:355	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	0	39	theme	oxide	45:49	arg1	anchoring					27:35	anchoring	27:35	anchoring	27:35	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	0	39	theme	oxide	45:49	arg1	synthesis					13:21	Sonochemical synthesis	0:21	Sonochemical synthesis	0:21	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	6	40	theme	lower	1176:1180	arg1	-0.2 V					1203:1208	-0.2 V	1203:1208	-0.2 V	1203:1208	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	40	theme	lower	1176:1180	arg1	potential					1192:1200	a lower reduction potential	1174:1200	a lower reduction potential (-0.2 V)	1174:1209	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	2	41	theme	oxide	343:347	arg1	nanorods					326:333	The hexagonal nanorods	312:333	The hexagonal nanorods of zinc oxide (ZnO NR)	312:356	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	5	42	theme	enhanced	911:918	arg1	behaviour					942:950	an enhanced redox electrochemical behaviour	908:950	an enhanced redox electrochemical behaviour of hemin	908:959	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	5	43	theme	hemin	1045:1049	arg1	electrodes					1060:1069	other hemin modified electrodes	1039:1069	other hemin modified electrodes	1039:1069	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	6	44	theme	electrocatalytic	1143:1158	arg1	activity					1160:1167	higher electrocatalytic activity	1136:1167	higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2	1136:1222	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	3	45	theme	stable	557:562	arg1	bio-composites					570:583	The stable hemin bio-composites	553:583	The stable hemin bio-composites	553:583	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	46	theme	electrochemical	726:740	arg1	detection					742:750	the selective and ultrasensitive electrochemical detection	693:750	the selective and ultrasensitive electrochemical detection of H2O2	693:758	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	5	47	theme	voltammetry	874:884	arg1	studies					886:892	Cyclic voltammetry studies	867:892	Cyclic voltammetry studies	867:892	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	7	48	theme	4.0 nM	1501:1506	arg1	limit					1492:1496	a lower detection limit	1474:1496	a lower detection limit of 4.0 nM	1474:1506	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	4	49	theme	NR	849:850	arg1	nanocomposite					852:864	MWCNT-CNC/ZnO NR nanocomposite	835:864	MWCNT-CNC/ZnO NR nanocomposite	835:864	The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite.					
31945552	7	50	theme	detection	1482:1490	arg1	limit					1492:1496	a lower detection limit	1474:1496	a lower detection limit of 4.0 nM	1474:1506	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	0	51	theme	H2O2	156:159	arg1	biosensing					142:151	ultra-sensitive biosensing	126:151	ultra-sensitive biosensing of H2O2	126:159	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	2	52	theme	hexagonal	316:324	arg1	nanorods					326:333	The hexagonal nanorods	312:333	The hexagonal nanorods of zinc oxide (ZnO NR)	312:356	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	3	53	theme	bath	614:617	arg1	sonication					619:628	bath sonication	614:628	the bath sonication (37 kHz of frequency, 150 W of power) method	610:673	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	54	dep	sonication	619:628	arg1	37 kHz					631:636	37 kHz	631:636	37 kHz	631:636	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	54	dep	sonication	619:628	arg1	power					661:665	power	661:665	power	661:665	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	54	dep	sonication	619:628	arg1	150 W					652:656	150 W	652:656	150 W	652:656	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	3	54	dep	sonication	619:628	arg1	frequency					641:649	frequency	641:649	frequency	641:649	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	1	55	theme	multiwalled	225:235	arg1	MWCNT					255:259	MWCNT	255:259	MWCNT	255:259	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	1	55	theme	multiwalled	225:235	arg1	nanotubes					244:252	multiwalled carbon nanotubes	225:252	multiwalled carbon nanotubes (MWCNT)	225:260	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	6	56	theme	modified	1262:1269	arg1	electrodes					1271:1280	other investigated hemin modified electrodes	1237:1280	other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO	1237:1320	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	56	theme	modified	1262:1269	arg1	hemin/CNC-ZnO					1308:1320	hemin/CNC-ZnO	1308:1320	hemin/CNC-ZnO	1308:1320	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	56	theme	modified	1262:1269	arg1	hemin/MWCNT					1292:1302	hemin/MWCNT	1292:1302	hemin/MWCNT	1292:1302	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	5	57	theme	hemin	955:959	arg1	behaviour					942:950	an enhanced redox electrochemical behaviour	908:950	an enhanced redox electrochemical behaviour of hemin	908:959	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	6	58	theme	investigated	1243:1254	arg1	electrodes					1271:1280	other investigated hemin modified electrodes	1237:1280	other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO	1237:1320	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	58	theme	investigated	1243:1254	arg1	hemin/CNC-ZnO					1308:1320	hemin/CNC-ZnO	1308:1320	hemin/CNC-ZnO	1308:1320	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	58	theme	investigated	1243:1254	arg1	hemin/MWCNT					1292:1302	hemin/MWCNT	1292:1302	hemin/MWCNT	1292:1302	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	0	59	theme	multiwalled	69:79	arg1	nanotubes-cellulose					88:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	4	60	from	presence	807:814	arg1	nanocomposite					852:864	MWCNT-CNC/ZnO NR nanocomposite	835:864	MWCNT-CNC/ZnO NR nanocomposite	835:864	The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite.					
31945552	0	61	from	anchoring	27:35	arg1	nanotubes-cellulose					88:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	5	62	theme	redox	920:924	arg1	behaviour					942:950	an enhanced redox electrochemical behaviour	908:950	an enhanced redox electrochemical behaviour of hemin	908:959	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	0	63	from	synthesis	13:21	arg1	nanotubes-cellulose					88:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	hemin-mediated multiwalled carbon nanotubes-cellulose	54:106	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	5	64	theme	MWCNT-CNC/ZnO	995:1007	arg1	nanocomposite					1012:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite	977:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes	977:1069	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	2	65	theme	first	541:545	arg1	time					547:550	the first time	537:550	the first time	537:550	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	4	66	theme	spectroscopy	772:783	arg1	studies					785:791	The UV-Vis spectroscopy studies	761:791	The UV-Vis spectroscopy studies	761:791	The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite.					
31945552	2	67	theme	sonication	384:393	arg1	method					432:437	probe sonication (frequency = 20 kHz, amplitude = 50) method	378:437	probe sonication (frequency = 20 kHz, amplitude = 50) method	378:437	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	5	68	theme	other	1039:1043	arg1	electrodes					1060:1069	other hemin modified electrodes	1039:1069	other hemin modified electrodes	1039:1069	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	1	69	theme	sonochemical	291:302	arg1	method					304:309	a simple sonochemical method	282:309	a simple sonochemical method	282:309	In this work, the metal oxide and biopolymer nanocomposites on multiwalled carbon nanotubes (MWCNT) were prepared using a simple sonochemical method.					
31945552	5	70	theme	hemin	977:981	arg1	nanocomposite					1012:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite	977:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes	977:1069	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	6	71	theme	NR/hemin	1096:1103	arg1	SPCE					1114:1117	the MWCNT-CNC/ZnO NR/hemin modified SPCE	1078:1117	the MWCNT-CNC/ZnO NR/hemin modified SPCE	1078:1117	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	2	72	theme	amplitude = 50	416:429	arg1	frequency = 20 kHz					396:413	frequency = 20 kHz	396:413	frequency = 20 kHz	396:413	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	0	73	theme	zinc	40:43	arg1	oxide					45:49	zinc oxide	40:49	zinc oxide	40:49	Sonochemical synthesis and anchoring of zinc oxide on hemin-mediated multiwalled carbon nanotubes-cellulose nanocomposite for ultra-sensitive biosensing of H2O2.					
31945552	5	74	theme	modified	1051:1058	arg1	electrodes					1060:1069	other hemin modified electrodes	1039:1069	other hemin modified electrodes	1039:1069	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	7	75	theme	stable	1360:1365	arg1	response					1380:1387	a stable amperometric response	1358:1387	a stable amperometric response (-0.2 V vs Ag/AgCl)	1358:1407	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	2	76	theme	functionalized	477:490	arg1	MWCNT-CNC					522:530	MWCNT-CNC	522:530	MWCNT-CNC	522:530	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	2	76	theme	functionalized	477:490	arg1	nanocrystals					508:519	ultrasonically functionalized MWCNT-cellulose nanocrystals	462:519	ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time	462:550	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	4	77	theme	native	819:824	arg1	hemin					826:830	native hemin	819:830	native hemin	819:830	The UV-Vis spectroscopy studies confirmed the presence of native hemin on MWCNT-CNC/ZnO NR nanocomposite.					
31945552	5	78	located	observed	965:972	arg1	nanocomposite					1012:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite	977:1024	hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes	977:1069	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	5	78	located	observed	965:972	arg2	behaviour					942:950	an enhanced redox electrochemical behaviour	908:950	an enhanced redox electrochemical behaviour of hemin	908:959	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	6	79	theme	reduction	1182:1190	arg1	-0.2 V					1203:1208	-0.2 V	1203:1208	-0.2 V	1203:1208	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	6	79	theme	reduction	1182:1190	arg1	potential					1192:1200	a lower reduction potential	1174:1200	a lower reduction potential (-0.2 V)	1174:1209	Also, the MWCNT-CNC/ZnO NR/hemin modified SPCE showed 2.3 folds higher electrocatalytic activity with a lower reduction potential (-0.2 V) towards H2O2 than that of other investigated hemin modified electrodes including hemin/MWCNT and hemin/CNC-ZnO.					
31945552	7	80	theme	fabricated	1327:1336	arg1	biosensor					1338:1346	The fabricated biosensor	1323:1346	The fabricated biosensor	1323:1346	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	3	81	used	used	684:687	arg2	bio-composites					570:583	The stable hemin bio-composites	553:583	The stable hemin bio-composites	553:583	The stable hemin bio-composites also were prepared using the bath sonication (37 kHz of frequency, 150 W of power) method, and was used for the selective and ultrasensitive electrochemical detection of H2O2.					
31945552	2	82	theme	zinc	338:341	arg1	oxide					343:347	zinc oxide	338:347	zinc oxide (ZnO NR)	338:356	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	2	82	theme	zinc	338:341	arg1	NR					354:355	ZnO NR	350:355	ZnO NR	350:355	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31945552	5	83	theme	Cyclic	867:872	arg1	studies					886:892	Cyclic voltammetry studies	867:892	Cyclic voltammetry studies	867:892	Cyclic voltammetry studies revealed that an enhanced redox electrochemical behaviour of hemin was observed on hemin immobilised MWCNT-CNC/ZnO NR nanocomposite than that of other hemin modified electrodes.					
31945552	7	84	dep	response	1380:1387	arg1	Ag/AgCl					1400:1406	Ag/AgCl	1400:1406	Ag/AgCl	1400:1406	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	7	84	dep	response	1380:1387	arg1	-0.2 V					1390:1395	-0.2 V	1390:1395	-0.2 V	1390:1395	The fabricated biosensor displayed a stable amperometric response (-0.2 V vs Ag/AgCl) in the linear concentration of H2O2 ranging up to 4183.3 µM with a lower detection limit of 4.0 nM.					
31945552	2	85	dep	sonication	384:393	arg1	frequency = 20 kHz					396:413	frequency = 20 kHz	396:413	frequency = 20 kHz	396:413	The hexagonal nanorods of zinc oxide (ZnO NR) were synthesized by probe sonication (frequency = 20 kHz, amplitude = 50) method and were integrated on ultrasonically functionalized MWCNT-cellulose nanocrystals (MWCNT-CNC) for the first time.					
31411620	8	0	theme	3	1076:1076	arg1	mg					1078:1079	mg	1078:1079	mg	1078:1079	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	6	1	theme	mucus	857:861	arg1	retention					831:839	The steric retention	820:839	The steric retention of pathological mucus	820:861	The steric retention of pathological mucus was reproduced by targeting its mesh size (approximately 50 nm) and viscoelastic properties.					
31411620	11	2	theme	step	1689:1692	arg1	forward					1694:1700	a step forward	1687:1700	a step forward to model the mucus layer	1687:1725	The mucus model can be proposed as an in vitro tool for early drug discovery, representing a step forward to model the mucus layer.					
31411620	4	3	theme	modular	655:661	arg1	approach					663:670	a modular approach	653:670	a modular approach	653:670	An airway CF mucus model is herein proposed to disassemble the complexity of the mucus barrier following a modular approach.					
31411620	0	4	theme	screening	65:73	arg1	tool					75:78	a fast screening tool	58:78	a fast screening tool for early drug discovery	58:103	Disassembling the complexity of mucus barriers to develop a fast screening tool for early drug discovery.					
31411620	6	5	theme	pathological	844:855	arg1	mucus					857:861	pathological mucus	844:861	pathological mucus	844:861	The steric retention of pathological mucus was reproduced by targeting its mesh size (approximately 50 nm) and viscoelastic properties.					
31411620	8	6	dep	20	1233:1234	arg1	to					1230:1231	to	1230:1231	to	1230:1231	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	0	7	theme	early	84:88	arg1	discovery					95:103	early drug discovery	84:103	early drug discovery	84:103	Disassembling the complexity of mucus barriers to develop a fast screening tool for early drug discovery.					
31411620	11	8	dep	in	1634:1635	arg1	vitro					1637:1641	vitro	1637:1641	vitro	1637:1641	The mucus model can be proposed as an in vitro tool for early drug discovery, representing a step forward to model the mucus layer.					
31411620	10	9	theme	layer	1410:1414	arg1	barrier					1391:1397	the steric barrier	1380:1397	the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion	1380:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	9	theme	layer	1410:1414	arg1	interaction					1364:1374	the mucin-drug interaction	1349:1374	the mucin-drug interaction	1349:1374	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	10	theme	mucus	1404:1408	arg1	layer					1410:1414	a mucus layer	1402:1414	a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion	1402:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	4	11	theme	CF	558:559	arg1	model					567:571	An airway CF mucus model	548:571	An airway CF mucus model	548:571	An airway CF mucus model is herein proposed to disassemble the complexity of the mucus barrier following a modular approach.					
31411620	8	12	theme	mg	1078:1079	arg1	Alg					1086:1088	3 mg ml-1 Alg	1076:1088	3 mg ml-1 Alg	1076:1088	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	12	13	theme	proposed	1746:1753	arg1	methodology					1755:1765	the proposed methodology	1742:1765	the proposed methodology	1742:1765	Additionally, the proposed methodology allows to easily include other molecules present within mucus, as relevant proteins, lipids and DNA.					
31411620	1	14	with	barrier	125:131	arg1	role					151:154	a protective role	138:154	a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites	138:288	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31411620	10	15	theme	artificial	1445:1454	arg1	PAMPA					1479:1483	PAMPA	1479:1483	PAMPA	1479:1483	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	15	theme	artificial	1445:1454	arg1	permeability					1465:1476	the parallel artificial membrane permeability	1432:1476	the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion	1432:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	4	16	theme	airway	551:556	arg1	model					567:571	An airway CF mucus model	548:571	An airway CF mucus model	548:571	An airway CF mucus model is herein proposed to disassemble the complexity of the mucus barrier following a modular approach.					
31411620	6	17	theme	steric	824:829	arg1	retention					831:839	The steric retention	820:839	The steric retention of pathological mucus	820:861	The steric retention of pathological mucus was reproduced by targeting its mesh size (approximately 50 nm) and viscoelastic properties.					
31411620	7	18	theme	CF	1030:1031	arg1	mucus					1033:1037	the CF mucus	1026:1037	the CF mucus	1026:1037	The interactive barrier was reproduced by a composition inspired from the CF mucus.					
31411620	8	19	theme	frequency	1207:1215	arg1	range					1217:1221	the frequency range	1203:1221	the frequency range of 0.1 to 20 Hz	1203:1237	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	4	20	theme	mucus	629:633	arg1	barrier					635:641	the mucus barrier	625:641	the mucus barrier	625:641	An airway CF mucus model is herein proposed to disassemble the complexity of the mucus barrier following a modular approach.					
31411620	1	21	theme	protective	140:149	arg1	role					151:154	a protective role	138:154	a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites	138:288	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31411620	0	22	theme	drug	90:93	arg1	discovery					95:103	early drug discovery	84:103	early drug discovery	84:103	Disassembling the complexity of mucus barriers to develop a fast screening tool for early drug discovery.					
31411620	10	23	theme	mucus	1304:1308	arg1	able					1320:1323	able	1320:1323	able	1320:1323	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	23	theme	mucus	1304:1308	arg1	model					1310:1314	The proposed mucus model	1291:1314	The proposed mucus model	1291:1314	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	4	24	theme	mucus	561:565	arg1	model					567:571	An airway CF mucus model	548:571	An airway CF mucus model	548:571	An airway CF mucus model is herein proposed to disassemble the complexity of the mucus barrier following a modular approach.					
31411620	9	25	theme	model	1278:1282	arg1	drugs					1284:1288	three model drugs	1272:1288	three model drugs	1272:1288	Drug diffusion was tested using three model drugs.					
31411620	8	26	theme	mg	1097:1098	arg1	mucin					1105:1109	25 mg ml-1 mucin	1094:1109	25 mg ml-1 mucin	1094:1109	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	5	27	theme	alginate	716:723	arg1	network					731:737	an alginate (Alg) network	713:737	an alginate (Alg) network	713:737	A hydrogel, mainly composed of mucin in an alginate (Alg) network, is proposed to specifically model the chemical-physical properties of CF mucus.					
31411620	10	28	theme	proposed	1295:1302	arg1	able					1320:1323	able	1320:1323	able	1320:1323	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	28	theme	proposed	1295:1302	arg1	model					1310:1314	The proposed mucus model	1291:1314	The proposed mucus model	1291:1314	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	12	29	theme	relevant	1833:1840	arg1	proteins					1842:1849	relevant proteins	1833:1849	relevant proteins	1833:1849	Additionally, the proposed methodology allows to easily include other molecules present within mucus, as relevant proteins, lipids and DNA.					
31411620	12	29	theme	relevant	1833:1840	arg1	molecules					1798:1806	other molecules	1792:1806	other molecules present within mucus	1792:1827	Additionally, the proposed methodology allows to easily include other molecules present within mucus, as relevant proteins, lipids and DNA.					
31411620	3	30	theme	unmet	531:535	arg1	challenge					537:545	an unmet challenge	528:545	an unmet challenge	528:545	Effectively modelling the passage through mucus still represents an unmet challenge.					
31411620	10	31	theme	mucin-drug	1353:1362	arg1	interaction					1364:1374	the mucin-drug interaction	1349:1374	the mucin-drug interaction	1349:1374	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	2	32	theme	pulmonary	330:338	arg1	mucus					340:344	pulmonary mucus	330:344	pulmonary mucus	330:344	In diseases like cystic fibrosis (CF), pulmonary mucus exhibits altered features, which hamper clearance mechanisms and drug diffusion, ultimately leading to lung failure.					
31411620	8	33	theme	25	1094:1095	arg1	mg					1097:1098	mg	1097:1098	mg	1097:1098	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	10	34	theme	parallel	1436:1443	arg1	PAMPA					1479:1483	PAMPA	1479:1483	PAMPA	1479:1483	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	34	theme	parallel	1436:1443	arg1	permeability					1465:1476	the parallel artificial membrane permeability	1432:1476	the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion	1432:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	5	35	theme	mucus	813:817	arg1	properties					796:805	the chemical-physical properties	774:805	the chemical-physical properties of CF mucus	774:817	A hydrogel, mainly composed of mucin in an alginate (Alg) network, is proposed to specifically model the chemical-physical properties of CF mucus.					
31411620	2	36	theme	clearance	386:394	arg1	mechanisms					396:405	clearance mechanisms	386:405	clearance mechanisms	386:405	In diseases like cystic fibrosis (CF), pulmonary mucus exhibits altered features, which hamper clearance mechanisms and drug diffusion, ultimately leading to lung failure.					
31411620	1	37	theme	effective	250:258	arg1	delivery					265:272	an effective drug delivery	247:272	an effective drug delivery to target sites	247:288	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31411620	8	38	dep	28.8	1192:1195	arg1	to					1189:1190	to	1189:1190	to	1189:1190	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	1	39	theme	drug	169:172	arg1	diffusion					174:182	drug diffusion	169:182	drug diffusion	169:182	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31411620	8	40	theme	ml-1	1100:1103	arg1	mucin					1105:1109	25 mg ml-1 mucin	1094:1109	25 mg ml-1 mucin	1094:1109	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	1	41	theme	drug	260:263	arg1	delivery					265:272	an effective drug delivery	247:272	an effective drug delivery to target sites	247:288	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31411620	8	42	dep	55.2	1152:1155	arg1	to					1149:1150	to	1149:1150	to	1149:1150	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	11	43	theme	mucus	1600:1604	arg1	model					1606:1610	The mucus model	1596:1610	The mucus model	1596:1610	The mucus model can be proposed as an in vitro tool for early drug discovery, representing a step forward to model the mucus layer.					
31411620	11	43	theme	mucus	1600:1604	arg1	tool					1643:1646	an in vitro tool	1631:1646	an in vitro tool for early drug discovery	1631:1671	The mucus model can be proposed as an in vitro tool for early drug discovery, representing a step forward to model the mucus layer.					
31411620	10	44	theme	screening	1553:1561	arg1	tool					1563:1566	the state-of-the-art screening tool	1532:1566	the state-of-the-art screening tool for passive drug diffusion	1532:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	44	theme	screening	1553:1561	arg1	membrane					1522:1529	the phospholipidic cell membrane	1498:1529	the phospholipidic cell membrane	1498:1529	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	12	45	theme	present	1808:1814	arg1	lipids					1852:1857	lipids	1852:1857	lipids	1852:1857	Additionally, the proposed methodology allows to easily include other molecules present within mucus, as relevant proteins, lipids and DNA.					
31411620	12	45	theme	present	1808:1814	arg1	proteins					1842:1849	relevant proteins	1833:1849	relevant proteins	1833:1849	Additionally, the proposed methodology allows to easily include other molecules present within mucus, as relevant proteins, lipids and DNA.					
31411620	12	45	theme	present	1808:1814	arg1	molecules					1798:1806	other molecules	1792:1806	other molecules present within mucus	1792:1827	Additionally, the proposed methodology allows to easily include other molecules present within mucus, as relevant proteins, lipids and DNA.					
31411620	10	46	theme	phospholipidic	1502:1515	arg1	tool					1563:1566	the state-of-the-art screening tool	1532:1566	the state-of-the-art screening tool for passive drug diffusion	1532:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	46	theme	phospholipidic	1502:1515	arg1	membrane					1522:1529	the phospholipidic cell membrane	1498:1529	the phospholipidic cell membrane	1498:1529	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	0	47	theme	barriers	38:45	arg1	complexity					18:27	the complexity	14:27	the complexity of mucus barriers	14:45	Disassembling the complexity of mucus barriers to develop a fast screening tool for early drug discovery.					
31411620	6	48	theme	mesh	895:898	arg1	size					900:903	its mesh size	891:903	its mesh size (approximately 50 nm)	891:925	The steric retention of pathological mucus was reproduced by targeting its mesh size (approximately 50 nm) and viscoelastic properties.					
31411620	6	48	theme	mesh	895:898	arg1	nm					923:924	approximately 50 nm	906:924	approximately 50 nm	906:924	The steric retention of pathological mucus was reproduced by targeting its mesh size (approximately 50 nm) and viscoelastic properties.					
31411620	8	49	theme	Hz	1236:1237	arg1	range					1217:1221	the frequency range	1203:1221	the frequency range of 0.1 to 20 Hz	1203:1237	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	0	50	theme	mucus	32:36	arg1	barriers					38:45	mucus barriers	32:45	mucus barriers	32:45	Disassembling the complexity of mucus barriers to develop a fast screening tool for early drug discovery.					
31411620	5	51	theme	CF	810:811	arg1	mucus					813:817	CF mucus	810:817	CF mucus	810:817	A hydrogel, mainly composed of mucin in an alginate (Alg) network, is proposed to specifically model the chemical-physical properties of CF mucus.					
31411620	6	52	theme	viscoelastic	931:942	arg1	properties					944:953	viscoelastic properties	931:953	viscoelastic properties	931:953	The steric retention of pathological mucus was reproduced by targeting its mesh size (approximately 50 nm) and viscoelastic properties.					
31411620	2	53	theme	altered	355:361	arg1	features					363:370	altered features	355:370	altered features	355:370	In diseases like cystic fibrosis (CF), pulmonary mucus exhibits altered features, which hamper clearance mechanisms and drug diffusion, ultimately leading to lung failure.					
31411620	8	54	theme	G	1166:1166	arg1	"					-1:-1		1164:-1	a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz	1164:1237	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	1	55	theme	target	277:282	arg1	sites					284:288	target sites	277:288	target sites	277:288	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31411620	8	56	theme	Optimized	1040:1048	arg1	models					1056:1061	Optimized mucus models	1040:1061	Optimized mucus models	1040:1061	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	10	57	theme	steric	1384:1389	arg1	barrier					1391:1397	the steric barrier	1380:1397	the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion	1380:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	58	theme	state-of-the-art	1536:1551	arg1	tool					1563:1566	the state-of-the-art screening tool	1532:1566	the state-of-the-art screening tool for passive drug diffusion	1532:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	58	theme	state-of-the-art	1536:1551	arg1	membrane					1522:1529	the phospholipidic cell membrane	1498:1529	the phospholipidic cell membrane	1498:1529	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	2	59	theme	cystic	308:313	arg1	CF					325:326	CF	325:326	CF	325:326	In diseases like cystic fibrosis (CF), pulmonary mucus exhibits altered features, which hamper clearance mechanisms and drug diffusion, ultimately leading to lung failure.					
31411620	2	59	theme	cystic	308:313	arg1	fibrosis					315:322	cystic fibrosis	308:322	cystic fibrosis (CF)	308:327	In diseases like cystic fibrosis (CF), pulmonary mucus exhibits altered features, which hamper clearance mechanisms and drug diffusion, ultimately leading to lung failure.					
31411620	2	60	theme	drug	411:414	arg1	diffusion					416:424	drug diffusion	411:424	drug diffusion	411:424	In diseases like cystic fibrosis (CF), pulmonary mucus exhibits altered features, which hamper clearance mechanisms and drug diffusion, ultimately leading to lung failure.					
31411620	10	61	theme	drug	1580:1583	arg1	diffusion					1585:1593	passive drug diffusion	1572:1593	passive drug diffusion	1572:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	12	62	theme	other	1792:1796	arg1	lipids					1852:1857	lipids	1852:1857	lipids	1852:1857	Additionally, the proposed methodology allows to easily include other molecules present within mucus, as relevant proteins, lipids and DNA.					
31411620	12	62	theme	other	1792:1796	arg1	proteins					1842:1849	relevant proteins	1833:1849	relevant proteins	1833:1849	Additionally, the proposed methodology allows to easily include other molecules present within mucus, as relevant proteins, lipids and DNA.					
31411620	12	62	theme	other	1792:1796	arg1	molecules					1798:1806	other molecules	1792:1806	other molecules present within mucus	1792:1827	Additionally, the proposed methodology allows to easily include other molecules present within mucus, as relevant proteins, lipids and DNA.					
31411620	7	63	theme	interactive	960:970	arg1	barrier					972:978	The interactive barrier	956:978	The interactive barrier	956:978	The interactive barrier was reproduced by a composition inspired from the CF mucus.					
31411620	11	64	theme	early	1652:1656	arg1	discovery					1663:1671	early drug discovery	1652:1671	early drug discovery	1652:1671	The mucus model can be proposed as an in vitro tool for early drug discovery, representing a step forward to model the mucus layer.					
31411620	5	65	theme	Alg	726:728	arg1	network					731:737	an alginate (Alg) network	713:737	an alginate (Alg) network	713:737	A hydrogel, mainly composed of mucin in an alginate (Alg) network, is proposed to specifically model the chemical-physical properties of CF mucus.					
31411620	11	66	theme	drug	1658:1661	arg1	discovery					1663:1671	early drug discovery	1652:1671	early drug discovery	1652:1671	The mucus model can be proposed as an in vitro tool for early drug discovery, representing a step forward to model the mucus layer.					
31411620	10	67	theme	passive	1572:1578	arg1	diffusion					1585:1593	passive drug diffusion	1572:1593	passive drug diffusion	1572:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	8	68	theme	ml-1	1081:1084	arg1	Alg					1086:1088	3 mg ml-1 Alg	1076:1088	3 mg ml-1 Alg	1076:1088	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	8	69	theme	mucus	1050:1054	arg1	models					1056:1061	Optimized mucus models	1040:1061	Optimized mucus models	1040:1061	Optimized mucus models, composed of 3 mg ml-1 Alg and 25 mg ml-1 mucin, exhibited a G' increasing from ∼21.2 to 55.2 Pa and a G'' ranging from ∼5.26 to 28.8 Pa in the frequency range of 0.1 to 20 Hz.					
31411620	5	70	from	mucin	704:708	arg1	network					731:737	an alginate (Alg) network	713:737	an alginate (Alg) network	713:737	A hydrogel, mainly composed of mucin in an alginate (Alg) network, is proposed to specifically model the chemical-physical properties of CF mucus.					
31411620	11	71	theme	in	1634:1635	arg1	tool					1643:1646	an in vitro tool	1631:1646	an in vitro tool for early drug discovery	1631:1671	The mucus model can be proposed as an in vitro tool for early drug discovery, representing a step forward to model the mucus layer.					
31411620	11	71	theme	in	1634:1635	arg1	model					1606:1610	The mucus model	1596:1610	The mucus model	1596:1610	The mucus model can be proposed as an in vitro tool for early drug discovery, representing a step forward to model the mucus layer.					
31411620	10	72	theme	membrane	1456:1463	arg1	PAMPA					1479:1483	PAMPA	1479:1483	PAMPA	1479:1483	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	72	theme	membrane	1456:1463	arg1	permeability					1465:1476	the parallel artificial membrane permeability	1432:1476	the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion	1432:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	1	73	theme	steric	200:205	arg1	barrier					223:229	a steric and interactive barrier	198:229	a steric and interactive barrier to overcome for an effective drug delivery to target sites	198:288	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31411620	0	74	theme	fast	60:63	arg1	tool					75:78	a fast screening tool	58:78	a fast screening tool for early drug discovery	58:103	Disassembling the complexity of mucus barriers to develop a fast screening tool for early drug discovery.					
31411620	5	75	theme	chemical-physical	778:794	arg1	properties					796:805	the chemical-physical properties	774:805	the chemical-physical properties of CF mucus	774:817	A hydrogel, mainly composed of mucin in an alginate (Alg) network, is proposed to specifically model the chemical-physical properties of CF mucus.					
31411620	10	76	theme	cell	1517:1520	arg1	tool					1563:1566	the state-of-the-art screening tool	1532:1566	the state-of-the-art screening tool for passive drug diffusion	1532:1593	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	10	76	theme	cell	1517:1520	arg1	membrane					1522:1529	the phospholipidic cell membrane	1498:1529	the phospholipidic cell membrane	1498:1529	The proposed mucus model was able to discriminate between the mucin-drug interaction and the steric barrier of a mucus layer with respect to the parallel artificial membrane permeability (PAMPA) that models the phospholipidic cell membrane, the state-of-the-art screening tool for passive drug diffusion.					
31411620	1	77	theme	natural	117:123	arg1	barrier					125:131	a natural barrier	115:131	a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites	115:288	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31411620	1	77	theme	natural	117:123	arg1	Mucus					106:110	Mucus	106:110	Mucus	106:110	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31411620	11	78	theme	mucus	1715:1719	arg1	layer					1721:1725	the mucus layer	1711:1725	the mucus layer	1711:1725	The mucus model can be proposed as an in vitro tool for early drug discovery, representing a step forward to model the mucus layer.					
31411620	2	79	theme	lung	449:452	arg1	failure					454:460	lung failure	449:460	lung failure	449:460	In diseases like cystic fibrosis (CF), pulmonary mucus exhibits altered features, which hamper clearance mechanisms and drug diffusion, ultimately leading to lung failure.					
31411620	0	80	dep	Disassembling	0:12	arg1	develop					50:56	develop	50:56	to develop a fast screening tool for early drug discovery	47:103	Disassembling the complexity of mucus barriers to develop a fast screening tool for early drug discovery.					
31411620	4	81	theme	barrier	635:641	arg1	complexity					611:620	the complexity	607:620	the complexity of the mucus barrier	607:641	An airway CF mucus model is herein proposed to disassemble the complexity of the mucus barrier following a modular approach.					
31411620	9	82	theme	Drug	1240:1243	arg1	diffusion					1245:1253	Drug diffusion	1240:1253	Drug diffusion	1240:1253	Drug diffusion was tested using three model drugs.					
31411620	1	83	theme	interactive	211:221	arg1	barrier					223:229	a steric and interactive barrier	198:229	a steric and interactive barrier to overcome for an effective drug delivery to target sites	198:288	Mucus is a natural barrier with a protective role that hinders drug diffusion, representing a steric and interactive barrier to overcome for an effective drug delivery to target sites.					
31165524	1	0	theme	insulin	200:206	arg1	injections					176:185	multiple daily injections	161:185	multiple daily injections of exogenous insulin	161:206	Insulin-dependent patients with diabetes mellitus require multiple daily injections of exogenous insulin to combat hyperglycemia.					
31165524	5	1	theme	glucose-dependent	839:855	arg1	change					864:869	glucose-dependent volume change	839:869	glucose-dependent volume change to facilitate glucagon release	839:900	The microgels incorporate zwitterionic moieties that stabilize loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release.					
31165524	6	2	theme	Hypoglycemia-triggered	903:924	arg1	release					926:932	Hypoglycemia-triggered release	903:932	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch	903:986	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch is demonstrated in vitro and in a rat model of type 1 diabetes (T1D).					
31165524	7	3	from	hypoglycemia	1121:1132	arg1	rats					1150:1153	the diabetic rats	1137:1153	the diabetic rats	1137:1153	Transdermal application of the patch prevented insulin-induced hypoglycemia in the diabetic rats.					
31165524	5	4	theme	glucagon	785:792	arg1	moieties					817:824	loaded glucagon and phenylboronic acid moieties	778:824	loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release	778:900	The microgels incorporate zwitterionic moieties that stabilize loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release.					
31165524	8	5	contain	has	1286:1288	arg2	potential					1303:1311	a tremendous potential	1290:1311	a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers	1290:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	8	5	contain	has	1286:1288	arg1	hypoglycemia					1266:1277	hypoglycemia	1266:1277	hypoglycemia	1266:1277	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	4	6	theme	methacrylated	619:631	arg1	acid					644:647	methacrylated hyaluronic acid	619:647	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels	599:712	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	4	6	theme	methacrylated	619:631	arg1	MeHA					650:653	MeHA	650:653	MeHA	650:653	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	6	7	from	patch	982:986	arg1	release					926:932	Hypoglycemia-triggered release	903:932	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch	903:986	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch is demonstrated in vitro and in a rat model of type 1 diabetes (T1D).					
31165524	3	8	theme	native	531:536	arg1	glucagon					538:545	native glucagon	531:545	native glucagon	531:545	To prevent hypoglycemia associated with intensive insulin therapy, a "smart" composite microneedle (cMN) patch is developed, which releases native glucagon at low glucose levels.					
31165524	4	9	theme	photo-crosslinked	601:617	arg1	array					668:672	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array	599:672	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels	599:712	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	5	10	theme	phenylboronic	798:810	arg1	acid					812:815	phenylboronic acid	798:815	phenylboronic acid	798:815	The microgels incorporate zwitterionic moieties that stabilize loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release.					
31165524	8	11	theme	hypoglycemia	1266:1277	arg1	prevention					1252:1261	the prevention	1248:1261	the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers	1248:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	5	12	theme	glucagon	885:892	arg1	release					894:900	glucagon release	885:900	glucagon release	885:900	The microgels incorporate zwitterionic moieties that stabilize loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release.					
31165524	3	13	theme	glucose	554:560	arg1	levels					562:567	low glucose levels	550:567	low glucose levels	550:567	To prevent hypoglycemia associated with intensive insulin therapy, a "smart" composite microneedle (cMN) patch is developed, which releases native glucagon at low glucose levels.					
31165524	4	14	theme	multifunctional	688:702	arg1	microgels					704:712	embedded multifunctional microgels	679:712	embedded multifunctional microgels	679:712	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	4	15	with	array	668:672	arg1	microgels					704:712	embedded multifunctional microgels	679:712	embedded multifunctional microgels	679:712	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	5	16	theme	volume	857:862	arg1	change					864:869	glucose-dependent volume change	839:869	glucose-dependent volume change to facilitate glucagon release	839:900	The microgels incorporate zwitterionic moieties that stabilize loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release.					
31165524	1	17	theme	Insulin-dependent	103:119	arg1	patients					121:128	Insulin-dependent patients	103:128	Insulin-dependent patients with diabetes mellitus	103:151	Insulin-dependent patients with diabetes mellitus require multiple daily injections of exogenous insulin to combat hyperglycemia.					
31165524	8	18	theme	first	1173:1177	arg1	work					1161:1164	This work	1156:1164	This work	1156:1164	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	8	18	theme	first	1173:1177	arg1	demonstration					1179:1191	the first demonstration	1169:1191	the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers	1169:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	4	19	theme	embedded	679:686	arg1	microgels					704:712	embedded multifunctional microgels	679:712	embedded multifunctional microgels	679:712	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	0	20	theme	Composite	19:27	arg1	Microneedle					29:39	Glucose-Responsive Composite Microneedle	0:39	Glucose-Responsive Composite Microneedle	0:39	Glucose-Responsive Composite Microneedle Patch for Hypoglycemia-Triggered Delivery of Native Glucagon.					
31165524	8	21	theme	patch	1238:1242	arg1	work					1161:1164	This work	1156:1164	This work	1156:1164	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	8	21	theme	patch	1238:1242	arg1	demonstration					1179:1191	the first demonstration	1169:1191	the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers	1169:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	8	22	theme	MN	1235:1236	arg1	patch					1238:1242	a glucose-responsive glucagon-delivery MN patch	1196:1242	a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers	1196:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	8	23	theme	intensive	1338:1346	arg1	therapy					1356:1362	intensive insulin therapy	1338:1362	intensive insulin therapy	1338:1362	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	0	24	theme	Glucose-Responsive	0:17	arg1	Microneedle					29:39	Glucose-Responsive Composite Microneedle	0:39	Glucose-Responsive Composite Microneedle	0:39	Glucose-Responsive Composite Microneedle Patch for Hypoglycemia-Triggered Delivery of Native Glucagon.					
31165524	2	25	theme	glucose	368:374	arg1	BGLs					384:387	BGLs	384:387	BGLs	384:387	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	2	25	theme	glucose	368:374	arg1	levels					376:381	abnormally low blood glucose levels	347:381	abnormally low blood glucose levels (BGLs)	347:388	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	1	26	with	patients	121:128	arg1	mellitus					144:151	diabetes mellitus	135:151	diabetes mellitus	135:151	Insulin-dependent patients with diabetes mellitus require multiple daily injections of exogenous insulin to combat hyperglycemia.					
31165524	4	27	theme	cMN	574:576	arg1	patch					578:582	The cMN patch	570:582	The cMN patch	570:582	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	2	28	theme	blood	362:366	arg1	BGLs					384:387	BGLs	384:387	BGLs	384:387	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	2	28	theme	blood	362:366	arg1	levels					376:381	abnormally low blood glucose levels	347:381	abnormally low blood glucose levels (BGLs)	347:388	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	3	29	theme	patch	496:500	arg1	"					466:466	a "smart"	458:466	a "smart" composite microneedle (cMN) patch	458:500	To prevent hypoglycemia associated with intensive insulin therapy, a "smart" composite microneedle (cMN) patch is developed, which releases native glucagon at low glucose levels.					
31165524	3	30	theme	intensive	431:439	arg1	therapy					449:455	intensive insulin therapy	431:455	intensive insulin therapy	431:455	To prevent hypoglycemia associated with intensive insulin therapy, a "smart" composite microneedle (cMN) patch is developed, which releases native glucagon at low glucose levels.					
31165524	6	31	theme	diabetes	1042:1049	arg1	model					1026:1030	a rat model	1020:1030	a rat model of type 1 diabetes (T1D)	1020:1055	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch is demonstrated in vitro and in a rat model of type 1 diabetes (T1D).					
31165524	8	32	theme	therapy	1356:1362	arg1	dangers					1327:1333	the dangers	1323:1333	the dangers of intensive insulin therapy	1323:1362	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	6	33	theme	cMN	978:980	arg1	patch					982:986	the cMN patch	974:986	the cMN patch	974:986	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch is demonstrated in vitro and in a rat model of type 1 diabetes (T1D).					
31165524	2	34	theme	low	358:360	arg1	BGLs					384:387	BGLs	384:387	BGLs	384:387	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	2	34	theme	low	358:360	arg1	levels					376:381	abnormally low blood glucose levels	347:381	abnormally low blood glucose levels (BGLs)	347:388	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	8	35	theme	insulin	1348:1354	arg1	therapy					1356:1362	intensive insulin therapy	1338:1362	intensive insulin therapy	1338:1362	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	0	36	theme	Hypoglycemia-Triggered	51:72	arg1	Delivery					74:81	Hypoglycemia-Triggered Delivery	51:81	Hypoglycemia-Triggered Delivery of Native Glucagon	51:100	Glucose-Responsive Composite Microneedle Patch for Hypoglycemia-Triggered Delivery of Native Glucagon.					
31165524	7	37	theme	diabetic	1141:1148	arg1	rats					1150:1153	the diabetic rats	1137:1153	the diabetic rats	1137:1153	Transdermal application of the patch prevented insulin-induced hypoglycemia in the diabetic rats.					
31165524	8	38	theme	life	1391:1394	arg1	quality					1380:1386	the quality	1376:1386	the quality of life of patients with diabetes and their caregivers	1376:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	6	39	theme	unchanged	950:958	arg1	glucagon					960:967	structurally unchanged glucagon	937:967	structurally unchanged glucagon	937:967	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch is demonstrated in vitro and in a rat model of type 1 diabetes (T1D).					
31165524	3	40	theme	smart	461:465	arg1	"					466:466	a "smart"	458:466	a "smart" composite microneedle (cMN) patch	458:500	To prevent hypoglycemia associated with intensive insulin therapy, a "smart" composite microneedle (cMN) patch is developed, which releases native glucagon at low glucose levels.					
31165524	6	41	theme	type	1035:1038	arg1	diabetes					1042:1049	type 1 diabetes	1035:1049	type 1 diabetes (T1D)	1035:1055	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch is demonstrated in vitro and in a rat model of type 1 diabetes (T1D).					
31165524	6	41	theme	type	1035:1038	arg1	T1D					1052:1054	T1D	1052:1054	T1D	1052:1054	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch is demonstrated in vitro and in a rat model of type 1 diabetes (T1D).					
31165524	5	42	theme	loaded	778:783	arg1	moieties					817:824	loaded glucagon and phenylboronic acid moieties	778:824	loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release	778:900	The microgels incorporate zwitterionic moieties that stabilize loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release.					
31165524	7	43	theme	patch	1089:1093	arg1	application					1070:1080	Transdermal application	1058:1080	Transdermal application of the patch	1058:1093	Transdermal application of the patch prevented insulin-induced hypoglycemia in the diabetic rats.					
31165524	5	44	theme	acid	812:815	arg1	moieties					817:824	loaded glucagon and phenylboronic acid moieties	778:824	loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release	778:900	The microgels incorporate zwitterionic moieties that stabilize loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release.					
31165524	8	45	theme	tremendous	1292:1301	arg1	potential					1303:1311	a tremendous potential	1290:1311	a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers	1290:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	6	46	theme	glucagon	960:967	arg1	release					926:932	Hypoglycemia-triggered release	903:932	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch	903:986	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch is demonstrated in vitro and in a rat model of type 1 diabetes (T1D).					
31165524	8	47	theme	glucose-responsive	1198:1215	arg1	patch					1238:1242	a glucose-responsive glucagon-delivery MN patch	1196:1242	a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers	1196:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	2	48	theme	excess	260:265	arg1	insulin					267:273	excess insulin	260:273	excess insulin	260:273	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	2	49	theme	insulin	267:273	arg1	administration					242:255	administration	242:255	administration of excess insulin	242:273	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	1	50	theme	multiple	161:168	arg1	injections					176:185	multiple daily injections	161:185	multiple daily injections of exogenous insulin	161:206	Insulin-dependent patients with diabetes mellitus require multiple daily injections of exogenous insulin to combat hyperglycemia.					
31165524	6	51	theme	rat	1022:1024	arg1	model					1026:1030	a rat model	1020:1030	a rat model of type 1 diabetes (T1D)	1020:1055	Hypoglycemia-triggered release of structurally unchanged glucagon from the cMN patch is demonstrated in vitro and in a rat model of type 1 diabetes (T1D).					
31165524	7	52	theme	insulin-induced	1105:1119	arg1	hypoglycemia					1121:1132	insulin-induced hypoglycemia	1105:1132	insulin-induced hypoglycemia in the diabetic rats	1105:1153	Transdermal application of the patch prevented insulin-induced hypoglycemia in the diabetic rats.					
31165524	4	53	link	photo-crosslinked	601:617	arg1	array					668:672	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array	599:672	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels	599:712	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	2	54	theme	life-threatening	303:318	arg1	condition					320:328	a life-threatening condition	301:328	a life-threatening condition characterized by abnormally low blood glucose levels (BGLs)	301:388	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	2	54	theme	life-threatening	303:318	arg1	hypoglycemia					287:298	hypoglycemia	287:298	hypoglycemia	287:298	However, administration of excess insulin can lead to hypoglycemia, a life-threatening condition characterized by abnormally low blood glucose levels (BGLs).					
31165524	1	55	theme	daily	170:174	arg1	injections					176:185	multiple daily injections	161:185	multiple daily injections of exogenous insulin	161:206	Insulin-dependent patients with diabetes mellitus require multiple daily injections of exogenous insulin to combat hyperglycemia.					
31165524	1	56	theme	diabetes	135:142	arg1	mellitus					144:151	diabetes mellitus	135:151	diabetes mellitus	135:151	Insulin-dependent patients with diabetes mellitus require multiple daily injections of exogenous insulin to combat hyperglycemia.					
31165524	4	57	theme	acid	644:647	arg1	array					668:672	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array	599:672	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels	599:712	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	5	58	theme	zwitterionic	741:752	arg1	moieties					754:761	zwitterionic moieties	741:761	zwitterionic moieties that stabilize loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release	741:900	The microgels incorporate zwitterionic moieties that stabilize loaded glucagon and phenylboronic acid moieties that provide glucose-dependent volume change to facilitate glucagon release.					
31165524	8	59	with	patients	1399:1406	arg1	caregivers					1432:1441	their caregivers	1426:1441	their caregivers	1426:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	8	59	with	patients	1399:1406	arg1	diabetes					1413:1420	diabetes	1413:1420	diabetes	1413:1420	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	7	60	theme	Transdermal	1058:1068	arg1	application					1070:1080	Transdermal application	1058:1080	Transdermal application of the patch	1058:1093	Transdermal application of the patch prevented insulin-induced hypoglycemia in the diabetic rats.					
31165524	1	61	theme	exogenous	190:198	arg1	insulin					200:206	exogenous insulin	190:206	exogenous insulin	190:206	Insulin-dependent patients with diabetes mellitus require multiple daily injections of exogenous insulin to combat hyperglycemia.					
31165524	8	62	theme	glucagon-delivery	1217:1233	arg1	patch					1238:1242	a glucose-responsive glucagon-delivery MN patch	1196:1242	a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers	1196:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	4	63	theme	hyaluronic	633:642	arg1	acid					644:647	methacrylated hyaluronic acid	619:647	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels	599:712	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	4	63	theme	hyaluronic	633:642	arg1	MeHA					650:653	MeHA	650:653	MeHA	650:653	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	0	64	theme	Glucagon	93:100	arg1	Delivery					74:81	Hypoglycemia-Triggered Delivery	51:81	Hypoglycemia-Triggered Delivery of Native Glucagon	51:100	Glucose-Responsive Composite Microneedle Patch for Hypoglycemia-Triggered Delivery of Native Glucagon.					
31165524	4	65	theme	microneedle	656:666	arg1	array					668:672	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array	599:672	a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels	599:712	The cMN patch is composed of a photo-crosslinked methacrylated hyaluronic acid (MeHA) microneedle array with embedded multifunctional microgels.					
31165524	8	66	theme	patients	1399:1406	arg1	quality					1380:1386	the quality	1376:1386	the quality of life of patients with diabetes and their caregivers	1376:1441	This work is the first demonstration of a glucose-responsive glucagon-delivery MN patch for the prevention of hypoglycemia, which has a tremendous potential to reduce the dangers of intensive insulin therapy and improve the quality of life of patients with diabetes and their caregivers.					
31165524	3	67	theme	insulin	441:447	arg1	therapy					449:455	intensive insulin therapy	431:455	intensive insulin therapy	431:455	To prevent hypoglycemia associated with intensive insulin therapy, a "smart" composite microneedle (cMN) patch is developed, which releases native glucagon at low glucose levels.					
31165524	0	68	theme	Native	86:91	arg1	Glucagon					93:100	Native Glucagon	86:100	Native Glucagon	86:100	Glucose-Responsive Composite Microneedle Patch for Hypoglycemia-Triggered Delivery of Native Glucagon.					
31165524	3	69	theme	low	550:552	arg1	levels					562:567	low glucose levels	550:567	low glucose levels	550:567	To prevent hypoglycemia associated with intensive insulin therapy, a "smart" composite microneedle (cMN) patch is developed, which releases native glucagon at low glucose levels.					
30939993	2	0	theme	ratio	473:477	arg1	composition					479:489	a 1:9 ratio composition	467:489	a 1:9 ratio composition with a high water-absorption rate	467:523	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	1	1	theme	sites	353:357	arg1	implantation					325:336	the implantation	321:336	the implantation of bone defect sites or poor bone quality in host bone	321:391	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	7	2	theme	bone	1043:1046	arg1	formation					1048:1056	new bone formation	1039:1056	new bone formation	1039:1056	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	6	3	theme	bone	832:835	arg1	formation					837:845	new bone formation	828:845	new bone formation ratio of the rhBMP-2 loaded composite group	828:889	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	2	4	theme	1:9	469:471	arg1	composition					479:489	a 1:9 ratio composition	467:489	a 1:9 ratio composition with a high water-absorption rate	467:523	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	6	5	theme	composite	875:883	arg1	group					885:889	the rhBMP-2 loaded composite group	856:889	the rhBMP-2 loaded composite group	856:889	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	2	6	dep	in	542:543	arg1	vivo					545:548	vivo	545:548	vivo	545:548	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	4	7	theme	bone	603:606	arg1	formation					608:616	new bone formation	599:616	new bone formation	599:616	The new bone formation and bone quantity and quality of fibular samples were examined.					
30939993	5	8	theme	marrow	800:805	arg1	space					807:811	the marrow space	796:811	the marrow space	796:811	The results showed that the rhBMP-2 powder gel composite improved the new bone formation in the cortical bone and the marrow space.					
30939993	0	9	theme	rhBMP-2	113:119	arg1	injection					121:129	rhBMP-2 injection	113:129	rhBMP-2 injection	113:129	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	1	10	theme	poor	362:365	arg1	quality					372:378	poor bone quality	362:378	poor bone quality	362:378	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	2	11	theme	water-absorption	503:518	arg1	rate					520:523	a high water-absorption rate	496:523	a high water-absorption rate	496:523	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	1	12	theme	bone	367:370	arg1	quality					372:378	poor bone quality	362:378	poor bone quality	362:378	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	1	13	from	implantation	325:336	arg1	bone					388:391	host bone	383:391	host bone	383:391	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	7	14	theme	segmental	1063:1071	arg1	defect					1087:1092	a segmental cortical bone defect	1061:1092	a segmental cortical bone defect after implantation	1061:1111	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	6	15	dep	loaded	868:873	arg1	rhBMP-2					860:866	rhBMP-2	860:866	rhBMP-2	860:866	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	2	16	theme	high	498:501	arg1	rate					520:523	a high water-absorption rate	496:523	a high water-absorption rate	496:523	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	1	17	theme	quality	372:378	arg1	implantation					325:336	the implantation	321:336	the implantation of bone defect sites or poor bone quality in host bone	321:391	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	1	18	from	carriers	309:316	arg1	implantation					325:336	the implantation	321:336	the implantation of bone defect sites or poor bone quality in host bone	321:391	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	2	19	with	composition	479:489	arg1	rate					520:523	a high water-absorption rate	496:523	a high water-absorption rate	496:523	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	2	20	theme	rhBMP-2	398:404	arg1	test					422:425	The rhBMP-2 release pattern test	394:425	The rhBMP-2 release pattern test	394:425	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	5	21	theme	powder	718:723	arg1	composite					729:737	the rhBMP-2 powder gel composite	706:737	the rhBMP-2 powder gel composite	706:737	The results showed that the rhBMP-2 powder gel composite improved the new bone formation in the cortical bone and the marrow space.					
30939993	6	22	theme	formation	837:845	arg1	ratio					847:851	new bone formation ratio	828:851	new bone formation ratio of the rhBMP-2 loaded composite group	828:889	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	5	23	theme	new	752:754	arg1	formation					761:769	the new bone formation	748:769	the new bone formation	748:769	The results showed that the rhBMP-2 powder gel composite improved the new bone formation in the cortical bone and the marrow space.					
30939993	1	24	theme	specific	190:197	arg1	objective					199:207	The specific objective	186:207	The specific objective of this study	186:221	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	1	25	theme	host	383:386	arg1	bone					388:391	host bone	383:391	host bone	383:391	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	7	26	theme	nice	985:988	arg1	composite					947:955	The composite	943:955	The composite of powder gel	943:969	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	7	26	theme	nice	985:988	arg1	gel					967:969	powder gel	960:969	powder gel	960:969	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	7	26	theme	nice	985:988	arg1	carrier					990:996	a nice carrier	983:996	a nice carrier	983:996	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	6	27	theme	gel	932:934	arg1	group					936:940	the powder gel group	921:940	the powder gel group	921:940	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	6	28	theme	ratio	847:851	arg1	length					818:823	The length	814:823	The length of new bone formation ratio of the rhBMP-2 loaded composite group	814:889	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	6	28	theme	ratio	847:851	arg1	higher					909:914	higher	909:914	higher	909:914	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	4	29	theme	bone	622:625	arg1	quantity					627:634	bone quantity	622:634	bone quantity	622:634	The new bone formation and bone quantity and quality of fibular samples were examined.					
30939993	5	30	theme	cortical	778:785	arg1	bone					787:790	the cortical bone	774:790	the cortical bone	774:790	The results showed that the rhBMP-2 powder gel composite improved the new bone formation in the cortical bone and the marrow space.					
30939993	1	31	theme	effective	291:299	arg1	carriers					309:316	effective rhBMP-2 carriers	291:316	effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone	291:391	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	1	31	theme	effective	291:299	arg1	bio-scaffolds					262:274	rhBMP-2-loaded bio-scaffolds	247:274	rhBMP-2-loaded bio-scaffolds	247:274	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	6	32	theme	group	885:889	arg1	ratio					847:851	new bone formation ratio	828:851	new bone formation ratio of the rhBMP-2 loaded composite group	828:889	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	7	33	theme	rhBMP-2	1019:1025	arg1	release					1008:1014	slow release	1003:1014	slow release of rhBMP-2	1003:1025	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	0	34	theme	phosphate	31:39	arg1	composite					86:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite	15:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation	15:144	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	1	35	theme	rhBMP-2	301:307	arg1	carriers					309:316	effective rhBMP-2 carriers	291:316	effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone	291:391	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	1	35	theme	rhBMP-2	301:307	arg1	bio-scaffolds					262:274	rhBMP-2-loaded bio-scaffolds	247:274	rhBMP-2-loaded bio-scaffolds	247:274	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	2	36	theme	in	542:543	arg1	study					550:554	in vivo study	542:554	in vivo study	542:554	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	0	37	theme	beta-tricalcium	15:29	arg1	composite					86:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite	15:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation	15:144	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	7	38	theme	slow	1003:1006	arg1	release					1008:1014	slow release	1003:1014	slow release of rhBMP-2	1003:1025	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	6	39	theme	new	828:830	arg1	formation					837:845	new bone formation	828:845	new bone formation ratio of the rhBMP-2 loaded composite group	828:889	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	6	40	theme	loaded	868:873	arg1	group					885:889	the rhBMP-2 loaded composite group	856:889	the rhBMP-2 loaded composite group	856:889	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	1	41	theme	study	217:221	arg1	objective					199:207	The specific objective	186:207	The specific objective of this study	186:221	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	0	42	theme	acid-based	64:73	arg1	composite					86:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite	15:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation	15:144	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	0	43	theme	bone	157:160	arg1	model					179:183	long bone segmental defect model	152:183	long bone segmental defect model	152:183	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	1	44	used	used	283:286	arg2	bio-scaffolds					262:274	rhBMP-2-loaded bio-scaffolds	247:274	rhBMP-2-loaded bio-scaffolds	247:274	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	1	44	used	used	283:286	arg2	carriers					309:316	effective rhBMP-2 carriers	291:316	effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone	291:391	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	2	45	theme	pattern	414:420	arg1	test					422:425	The rhBMP-2 release pattern test	394:425	The rhBMP-2 release pattern test	394:425	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	0	46	theme	microsphere-hyaluronic	41:62	arg1	composite					86:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite	15:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation	15:144	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	0	47	theme	long	152:155	arg1	model					179:183	long bone segmental defect model	152:183	long bone segmental defect model	152:183	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	4	48	theme	new	599:601	arg1	formation					608:616	new bone formation	599:616	new bone formation	599:616	The new bone formation and bone quantity and quality of fibular samples were examined.					
30939993	7	49	theme	gel	967:969	arg1	composite					947:955	The composite	943:955	The composite of powder gel	943:969	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	7	49	theme	gel	967:969	arg1	gel					967:969	powder gel	960:969	powder gel	960:969	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	7	49	theme	gel	967:969	arg1	carrier					990:996	a nice carrier	983:996	a nice carrier	983:996	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	2	50	theme	release	406:412	arg1	test					422:425	The rhBMP-2 release pattern test	394:425	The rhBMP-2 release pattern test	394:425	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	0	51	theme	gel	82:84	arg1	composite					86:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite	15:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation	15:144	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	0	52	theme	defect	172:177	arg1	model					179:183	long bone segmental defect model	152:183	long bone segmental defect model	152:183	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	6	53	theme	powder	925:930	arg1	group					936:940	the powder gel group	921:940	the powder gel group	921:940	The length of new bone formation ratio of the rhBMP-2 loaded composite group was significantly higher than the powder gel group.					
30939993	4	54	dep	formation	608:616	arg1	The					595:597	The	595:597	The	595:597	The new bone formation and bone quantity and quality of fibular samples were examined.					
30939993	0	55	theme	powder	75:80	arg1	composite					86:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite	15:94	beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation	15:144	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	0	56	theme	segmental	162:170	arg1	model					179:183	long bone segmental defect model	152:183	long bone segmental defect model	152:183	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	1	57	theme	bone	341:344	arg1	sites					353:357	bone defect sites	341:357	bone defect sites	341:357	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	5	58	theme	bone	756:759	arg1	formation					761:769	the new bone formation	748:769	the new bone formation	748:769	The results showed that the rhBMP-2 powder gel composite improved the new bone formation in the cortical bone and the marrow space.					
30939993	7	59	theme	powder	960:965	arg1	gel					967:969	powder gel	960:969	powder gel	960:969	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	5	60	theme	rhBMP-2	710:716	arg1	composite					729:737	the rhBMP-2 powder gel composite	706:737	the rhBMP-2 powder gel composite	706:737	The results showed that the rhBMP-2 powder gel composite improved the new bone formation in the cortical bone and the marrow space.					
30939993	7	61	theme	bone	1082:1085	arg1	defect					1087:1092	a segmental cortical bone defect	1061:1092	a segmental cortical bone defect after implantation	1061:1111	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	5	62	theme	gel	725:727	arg1	composite					729:737	the rhBMP-2 powder gel composite	706:737	the rhBMP-2 powder gel composite	706:737	The results showed that the rhBMP-2 powder gel composite improved the new bone formation in the cortical bone and the marrow space.					
30939993	4	63	theme	samples	659:665	arg1	quality					640:646	quality	640:646	quality	640:646	The new bone formation and bone quantity and quality of fibular samples were examined.					
30939993	4	63	theme	samples	659:665	arg1	formation					608:616	new bone formation	599:616	new bone formation	599:616	The new bone formation and bone quantity and quality of fibular samples were examined.					
30939993	4	63	theme	samples	659:665	arg1	quantity					627:634	bone quantity	622:634	bone quantity	622:634	The new bone formation and bone quantity and quality of fibular samples were examined.					
30939993	7	64	theme	cortical	1073:1080	arg1	defect					1087:1092	a segmental cortical bone defect	1061:1092	a segmental cortical bone defect after implantation	1061:1111	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
30939993	1	65	theme	rhBMP-2-loaded	247:260	arg1	carriers					309:316	effective rhBMP-2 carriers	291:316	effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone	291:391	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	1	65	theme	rhBMP-2-loaded	247:260	arg1	bio-scaffolds					262:274	rhBMP-2-loaded bio-scaffolds	247:274	rhBMP-2-loaded bio-scaffolds	247:274	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	0	66	theme	composite	86:94	arg1	Preparation					0:10	Preparation	0:10	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation	0:144	Preparation of beta-tricalcium phosphate microsphere-hyaluronic acid-based powder gel composite as a carrier for rhBMP-2 injection and evaluation using long bone segmental defect model.					
30939993	1	67	theme	defect	346:351	arg1	sites					353:357	bone defect sites	341:357	bone defect sites	341:357	The specific objective of this study was to evaluate whether rhBMP-2-loaded bio-scaffolds can be used as effective rhBMP-2 carriers in the implantation of bone defect sites or poor bone quality in host bone.					
30939993	2	68	theme	slow	434:437	arg1	results					439:445	slow results	434:445	slow results	434:445	The rhBMP-2 release pattern test showed slow results in both groups, and a 1:9 ratio composition with a high water-absorption rate was selected for in vivo study.					
30939993	4	69	theme	fibular	651:657	arg1	samples					659:665	fibular samples	651:665	fibular samples	651:665	The new bone formation and bone quantity and quality of fibular samples were examined.					
30939993	7	70	theme	new	1039:1041	arg1	formation					1048:1056	new bone formation	1039:1056	new bone formation	1039:1056	The composite of powder gel seems to be a nice carrier, and slow release of rhBMP-2 can promote new bone formation in a segmental cortical bone defect after implantation.					
31401480	0	0	theme	photoacoustic	86:98	arg1	imaging					100:106	photoacoustic imaging	86:106	photoacoustic imaging	86:106	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	2	1	theme	quantum	493:499	arg1	dots					501:504	CuS quantum dots	489:504	CuS quantum dots (QDs)	489:510	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	2	1	theme	quantum	493:499	arg1	QDs					507:509	QDs	507:509	QDs	507:509	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	7	2	theme	photothermal	962:973	arg1	efficiency					975:984	high photothermal efficiency	957:984	high photothermal efficiency	957:984	CuS-CS-FA QDs exhibit strong near-infrared photoabsorption and high photothermal efficiency (47.0%).					
31401480	2	3	theme	CuS	489:491	arg1	dots					501:504	CuS quantum dots	489:504	CuS quantum dots (QDs)	489:510	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	2	3	theme	CuS	489:491	arg1	QDs					507:509	QDs	507:509	QDs	507:509	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	9	4	theme	CuS-CS-FA	1267:1275	arg1	QD					1277:1278	CuS-CS-FA QD	1267:1278	CuS-CS-FA QD	1267:1278	When CuS-CS-FA QD dispersion is injected intravenously into the tumor-bearing mice, the photoacoustic imaging reveals that CuS-CS-FA QD can be efficiently targeted and accumulated in the tumor and reach the peak dose at 60 min.					
31401480	1	5	theme	artificial	176:185	arg1	organic-molecules					187:203	artificial organic-molecules	176:203	artificial organic-molecules	176:203	CuS nanomaterials capped with artificial organic-molecules or polymers have been well demonstrated as efficient photothermal nanoagents for the therapy of tumor, but their biocompatibility and target ability should be improved.					
31401480	5	6	theme	FA	698:699	arg1	process					714:720	FA modification process	698:720	FA modification process	698:720	FA modification process has no apparent influence on the size, phase and composition of the QDs.					
31401480	6	7	theme	efficient	865:873	arg1	conjugation					875:885	the efficient conjugation	861:885	the efficient conjugation of FA	861:891	Furthermore, the zeta potential and infrared spectroscopy confirm the efficient conjugation of FA.					
31401480	10	8	theme	laser	1399:1403	arg1	irradiation					1376:1386	The irradiation	1372:1386	The irradiation of 1064-nm laser (1.0 W cm-2, 10 min)	1372:1424	The irradiation of 1064-nm laser (1.0 W cm-2, 10 min) results in the efficient inhibition of tumor growth, without treatment-induced toxicity.					
31401480	9	9	theme	CuS-CS-FA	1149:1157	arg1	dispersion					1162:1171	CuS-CS-FA QD dispersion	1149:1171	CuS-CS-FA QD dispersion	1149:1171	When CuS-CS-FA QD dispersion is injected intravenously into the tumor-bearing mice, the photoacoustic imaging reveals that CuS-CS-FA QD can be efficiently targeted and accumulated in the tumor and reach the peak dose at 60 min.					
31401480	11	10	theme	biocompatible	1571:1583	arg1	nanoagents					1601:1610	biocompatible multifunctional nanoagents	1571:1610	biocompatible multifunctional nanoagents for imaging guided therapy of tumor	1571:1646	Therefore, CuS-CS-FA QDs have great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor.					
31401480	8	11	theme	cellular	1118:1125	arg1	uptake					1127:1132	cellular uptake	1118:1132	relatively high cellular uptake efficacy	1102:1141	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	5	12	theme	modification	701:712	arg1	process					714:720	FA modification process	698:720	FA modification process	698:720	FA modification process has no apparent influence on the size, phase and composition of the QDs.					
31401480	5	13	contain	has	722:724	arg1	process					714:720	FA modification process	698:720	FA modification process	698:720	FA modification process has no apparent influence on the size, phase and composition of the QDs.					
31401480	5	13	contain	has	722:724	arg2	influence					738:746	no apparent influence	726:746	no apparent influence	726:746	FA modification process has no apparent influence on the size, phase and composition of the QDs.					
31401480	10	14	theme	1064-nm	1391:1397	arg1	laser					1399:1403	1064-nm laser	1391:1403	1064-nm laser (1.0 W cm-2, 10 min)	1391:1424	The irradiation of 1064-nm laser (1.0 W cm-2, 10 min) results in the efficient inhibition of tumor growth, without treatment-induced toxicity.					
31401480	7	15	theme	strong	916:921	arg1	%					991:991	47.0%	987:991	47.0%	987:991	CuS-CS-FA QDs exhibit strong near-infrared photoabsorption and high photothermal efficiency (47.0%).					
31401480	7	15	theme	strong	916:921	arg1	photoabsorption					937:951	strong near-infrared photoabsorption	916:951	strong near-infrared photoabsorption	916:951	CuS-CS-FA QDs exhibit strong near-infrared photoabsorption and high photothermal efficiency (47.0%).					
31401480	10	16	theme	efficient	1441:1449	arg1	inhibition					1451:1460	the efficient inhibition	1437:1460	the efficient inhibition of tumor growth	1437:1476	The irradiation of 1064-nm laser (1.0 W cm-2, 10 min) results in the efficient inhibition of tumor growth, without treatment-induced toxicity.					
31401480	1	17	theme	tumor	301:305	arg1	therapy					290:296	the therapy	286:296	the therapy of tumor	286:305	CuS nanomaterials capped with artificial organic-molecules or polymers have been well demonstrated as efficient photothermal nanoagents for the therapy of tumor, but their biocompatibility and target ability should be improved.					
31401480	8	18	theme	presence	1014:1021	arg1	result					1000:1005	a result	998:1005	a result of the presence of CS ligand and FA modification	998:1054	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	18	theme	presence	1014:1021	arg1	biocompatibility					1081:1096	good biocompatibility	1076:1096	good biocompatibility	1076:1096	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	18	theme	presence	1014:1021	arg1	efficacy					1134:1141	relatively high cellular uptake efficacy	1102:1141	relatively high cellular uptake efficacy	1102:1141	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	7	19	theme	near-infrared	923:935	arg1	%					991:991	47.0%	987:991	47.0%	987:991	CuS-CS-FA QDs exhibit strong near-infrared photoabsorption and high photothermal efficiency (47.0%).					
31401480	7	19	theme	near-infrared	923:935	arg1	photoabsorption					937:951	strong near-infrared photoabsorption	916:951	strong near-infrared photoabsorption	916:951	CuS-CS-FA QDs exhibit strong near-infrared photoabsorption and high photothermal efficiency (47.0%).					
31401480	11	20	theme	guided	1624:1629	arg1	therapy					1631:1637	imaging guided therapy	1616:1637	imaging guided therapy of tumor	1616:1646	Therefore, CuS-CS-FA QDs have great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor.					
31401480	0	21	theme	photothermal	115:126	arg1	therapy					128:134	photothermal therapy	115:134	photothermal therapy of tumor	115:143	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	4	22	with	nanodots	663:670	arg1	sizes					677:681	sizes	677:681	sizes of about 4 nm	677:695	The resulting CuS-CS-FA QDs are composed of hexagonal phase nanodots with sizes of about 4 nm.					
31401480	11	23	theme	CuS-CS-FA	1526:1534	arg1	QDs					1536:1538	CuS-CS-FA QDs	1526:1538	CuS-CS-FA QDs	1526:1538	Therefore, CuS-CS-FA QDs have great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor.					
31401480	9	24	theme	tumor-bearing	1208:1220	arg1	mice					1222:1225	the tumor-bearing mice	1204:1225	the tumor-bearing mice	1204:1225	When CuS-CS-FA QD dispersion is injected intravenously into the tumor-bearing mice, the photoacoustic imaging reveals that CuS-CS-FA QD can be efficiently targeted and accumulated in the tumor and reach the peak dose at 60 min.					
31401480	0	25	theme	green	18:22	arg1	synthesis					24:32	Chitosan-mediated green synthesis	0:32	Chitosan-mediated green synthesis	0:32	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	6	26	theme	infrared	831:838	arg1	spectroscopy					840:851	infrared spectroscopy	831:851	infrared spectroscopy	831:851	Furthermore, the zeta potential and infrared spectroscopy confirm the efficient conjugation of FA.					
31401480	8	27	theme	CS	1026:1027	arg1	ligand					1029:1034	CS ligand and FA modification	1026:1054	ligand	1029:1034	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	4	28	theme	hexagonal	647:655	arg1	nanodots					663:670	hexagonal phase nanodots	647:670	hexagonal phase nanodots with sizes of about 4 nm	647:695	The resulting CuS-CS-FA QDs are composed of hexagonal phase nanodots with sizes of about 4 nm.					
31401480	0	29	theme	Chitosan-mediated	0:16	arg1	synthesis					24:32	Chitosan-mediated green synthesis	0:32	Chitosan-mediated green synthesis	0:32	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	4	30	theme	4 nm	692:695	arg1	sizes					677:681	sizes	677:681	sizes of about 4 nm	677:695	The resulting CuS-CS-FA QDs are composed of hexagonal phase nanodots with sizes of about 4 nm.					
31401480	2	31	theme	biomacromolecule	435:450	arg1	model					452:456	the biomacromolecule model	431:456	the biomacromolecule model	431:456	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	2	31	theme	biomacromolecule	435:450	arg1	chitosan					414:421	chitosan	414:421	chitosan (CS)	414:426	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	8	32	theme	uptake	1127:1132	arg1	result					1000:1005	a result	998:1005	a result of the presence of CS ligand and FA modification	998:1054	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	32	theme	uptake	1127:1132	arg1	biocompatibility					1081:1096	good biocompatibility	1076:1096	good biocompatibility	1076:1096	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	32	theme	uptake	1127:1132	arg1	efficacy					1134:1141	relatively high cellular uptake efficacy	1102:1141	relatively high cellular uptake efficacy	1102:1141	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	0	33	theme	tumor	139:143	arg1	therapy					128:134	photothermal therapy	115:134	photothermal therapy of tumor	115:143	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	7	34	theme	CuS-CS-FA	894:902	arg1	QDs					904:906	CuS-CS-FA QDs	894:906	CuS-CS-FA QDs	894:906	CuS-CS-FA QDs exhibit strong near-infrared photoabsorption and high photothermal efficiency (47.0%).					
31401480	3	35	theme	CuS-CS	550:555	arg1	QDs					557:559	CuS-CS QDs	550:559	CuS-CS QDs	550:559	CuS-CS QDs are then conjugated with folic acid (FA).					
31401480	11	36	theme	tumor	1642:1646	arg1	therapy					1631:1637	imaging guided therapy	1616:1637	imaging guided therapy of tumor	1616:1646	Therefore, CuS-CS-FA QDs have great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor.					
31401480	4	37	theme	resulting	607:615	arg1	QDs					627:629	The resulting CuS-CS-FA QDs	603:629	The resulting CuS-CS-FA QDs	603:629	The resulting CuS-CS-FA QDs are composed of hexagonal phase nanodots with sizes of about 4 nm.					
31401480	9	38	theme	peak	1351:1354	arg1	dose					1356:1359	the peak dose	1347:1359	the peak dose	1347:1359	When CuS-CS-FA QD dispersion is injected intravenously into the tumor-bearing mice, the photoacoustic imaging reveals that CuS-CS-FA QD can be efficiently targeted and accumulated in the tumor and reach the peak dose at 60 min.					
31401480	8	39	theme	ligand	1029:1034	arg1	presence					1014:1021	the presence	1010:1021	the presence of CS ligand and FA modification	1010:1054	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	10	40	dep	laser	1399:1403	arg1	10 min					1418:1423	10 min	1418:1423	10 min	1418:1423	The irradiation of 1064-nm laser (1.0 W cm-2, 10 min) results in the efficient inhibition of tumor growth, without treatment-induced toxicity.					
31401480	10	40	dep	laser	1399:1403	arg1	1.0 W cm-2					1406:1415	1.0 W cm-2	1406:1415	1.0 W cm-2	1406:1415	The irradiation of 1064-nm laser (1.0 W cm-2, 10 min) results in the efficient inhibition of tumor growth, without treatment-induced toxicity.					
31401480	2	41	theme	co-precipitation	525:540	arg1	method					542:547	a simple co-precipitation method	516:547	a simple co-precipitation method	516:547	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	3	42	theme	folic	586:590	arg1	FA					598:599	FA	598:599	FA	598:599	CuS-CS QDs are then conjugated with folic acid (FA).					
31401480	3	42	theme	folic	586:590	arg1	acid					592:595	folic acid	586:595	folic acid (FA)	586:600	CuS-CS QDs are then conjugated with folic acid (FA).					
31401480	0	43	theme	folic-acid	38:47	arg1	modification					49:60	folic-acid modification	38:60	folic-acid modification	38:60	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	2	44	theme	simple	518:523	arg1	method					542:547	a simple co-precipitation method	516:547	a simple co-precipitation method	516:547	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	6	45	theme	zeta	812:815	arg1	potential					817:825	the zeta potential	808:825	the zeta potential	808:825	Furthermore, the zeta potential and infrared spectroscopy confirm the efficient conjugation of FA.					
31401480	4	46	theme	CuS-CS-FA	617:625	arg1	QDs					627:629	The resulting CuS-CS-FA QDs	603:629	The resulting CuS-CS-FA QDs	603:629	The resulting CuS-CS-FA QDs are composed of hexagonal phase nanodots with sizes of about 4 nm.					
31401480	0	47	theme	CuS	65:67	arg1	dots					77:80	CuS quantum dots	65:80	CuS quantum dots	65:80	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	1	48	theme	target	339:344	arg1	ability					346:352	target ability	339:352	target ability	339:352	CuS nanomaterials capped with artificial organic-molecules or polymers have been well demonstrated as efficient photothermal nanoagents for the therapy of tumor, but their biocompatibility and target ability should be improved.					
31401480	8	49	theme	CuS-CS-FA	1057:1065	arg1	QDs					1067:1069	CuS-CS-FA QDs	1057:1069	CuS-CS-FA QDs	1057:1069	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	5	50	theme	apparent	729:736	arg1	influence					738:746	no apparent influence	726:746	no apparent influence	726:746	FA modification process has no apparent influence on the size, phase and composition of the QDs.					
31401480	10	51	theme	tumor	1465:1469	arg1	growth					1471:1476	tumor growth	1465:1476	tumor growth	1465:1476	The irradiation of 1064-nm laser (1.0 W cm-2, 10 min) results in the efficient inhibition of tumor growth, without treatment-induced toxicity.					
31401480	11	52	theme	imaging	1616:1622	arg1	therapy					1631:1637	imaging guided therapy	1616:1637	imaging guided therapy of tumor	1616:1646	Therefore, CuS-CS-FA QDs have great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor.					
31401480	2	53	theme	surface	462:468	arg1	ligands					470:476	surface ligands	462:476	surface ligands	462:476	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	2	53	theme	surface	462:468	arg1	chitosan					414:421	chitosan	414:421	chitosan (CS)	414:426	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	10	54	theme	growth	1471:1476	arg1	inhibition					1451:1460	the efficient inhibition	1437:1460	the efficient inhibition of tumor growth	1437:1476	The irradiation of 1064-nm laser (1.0 W cm-2, 10 min) results in the efficient inhibition of tumor growth, without treatment-induced toxicity.					
31401480	10	55	theme	treatment-induced	1487:1503	arg1	toxicity					1505:1512	treatment-induced toxicity	1487:1512	treatment-induced toxicity	1487:1512	The irradiation of 1064-nm laser (1.0 W cm-2, 10 min) results in the efficient inhibition of tumor growth, without treatment-induced toxicity.					
31401480	8	56	contain	have	1071:1074	arg2	efficacy					1134:1141	relatively high cellular uptake efficacy	1102:1141	relatively high cellular uptake efficacy	1102:1141	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	56	contain	have	1071:1074	arg1	QDs					1067:1069	CuS-CS-FA QDs	1057:1069	CuS-CS-FA QDs	1057:1069	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	56	contain	have	1071:1074	arg2	biocompatibility					1081:1096	good biocompatibility	1076:1096	good biocompatibility	1076:1096	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	56	contain	have	1071:1074	arg2	result					1000:1005	a result	998:1005	a result of the presence of CS ligand and FA modification	998:1054	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	57	theme	modification	1043:1054	arg1	presence					1014:1021	the presence	1010:1021	the presence of CS ligand and FA modification	1010:1054	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	9	58	theme	QD	1159:1160	arg1	dispersion					1162:1171	CuS-CS-FA QD dispersion	1149:1171	CuS-CS-FA QD dispersion	1149:1171	When CuS-CS-FA QD dispersion is injected intravenously into the tumor-bearing mice, the photoacoustic imaging reveals that CuS-CS-FA QD can be efficiently targeted and accumulated in the tumor and reach the peak dose at 60 min.					
31401480	11	59	theme	great	1545:1549	arg1	potential					1551:1559	great potential	1545:1559	great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor	1545:1646	Therefore, CuS-CS-FA QDs have great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor.					
31401480	7	60	theme	high	957:960	arg1	efficiency					975:984	high photothermal efficiency	957:984	high photothermal efficiency	957:984	CuS-CS-FA QDs exhibit strong near-infrared photoabsorption and high photothermal efficiency (47.0%).					
31401480	1	61	theme	efficient	248:256	arg1	nanoagents					271:280	efficient photothermal nanoagents	248:280	efficient photothermal nanoagents for the therapy of tumor	248:305	CuS nanomaterials capped with artificial organic-molecules or polymers have been well demonstrated as efficient photothermal nanoagents for the therapy of tumor, but their biocompatibility and target ability should be improved.					
31401480	1	61	theme	efficient	248:256	arg1	nanomaterials					150:162	CuS nanomaterials	146:162	CuS nanomaterials capped with artificial organic-molecules or polymers	146:215	CuS nanomaterials capped with artificial organic-molecules or polymers have been well demonstrated as efficient photothermal nanoagents for the therapy of tumor, but their biocompatibility and target ability should be improved.					
31401480	11	62	contain	have	1540:1543	arg2	potential					1551:1559	great potential	1545:1559	great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor	1545:1646	Therefore, CuS-CS-FA QDs have great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor.					
31401480	11	62	contain	have	1540:1543	arg1	QDs					1536:1538	CuS-CS-FA QDs	1526:1538	CuS-CS-FA QDs	1526:1538	Therefore, CuS-CS-FA QDs have great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor.					
31401480	9	63	theme	photoacoustic	1232:1244	arg1	imaging					1246:1252	the photoacoustic imaging	1228:1252	the photoacoustic imaging	1228:1252	When CuS-CS-FA QD dispersion is injected intravenously into the tumor-bearing mice, the photoacoustic imaging reveals that CuS-CS-FA QD can be efficiently targeted and accumulated in the tumor and reach the peak dose at 60 min.					
31401480	11	64	theme	multifunctional	1585:1599	arg1	nanoagents					1601:1610	biocompatible multifunctional nanoagents	1571:1610	biocompatible multifunctional nanoagents for imaging guided therapy of tumor	1571:1646	Therefore, CuS-CS-FA QDs have great potential to become biocompatible multifunctional nanoagents for imaging guided therapy of tumor.					
31401480	1	65	theme	CuS	146:148	arg1	nanoagents					271:280	efficient photothermal nanoagents	248:280	efficient photothermal nanoagents for the therapy of tumor	248:305	CuS nanomaterials capped with artificial organic-molecules or polymers have been well demonstrated as efficient photothermal nanoagents for the therapy of tumor, but their biocompatibility and target ability should be improved.					
31401480	1	65	theme	CuS	146:148	arg1	nanomaterials					150:162	CuS nanomaterials	146:162	CuS nanomaterials capped with artificial organic-molecules or polymers	146:215	CuS nanomaterials capped with artificial organic-molecules or polymers have been well demonstrated as efficient photothermal nanoagents for the therapy of tumor, but their biocompatibility and target ability should be improved.					
31401480	8	66	theme	good	1076:1079	arg1	result					1000:1005	a result	998:1005	a result of the presence of CS ligand and FA modification	998:1054	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	66	theme	good	1076:1079	arg1	biocompatibility					1081:1096	good biocompatibility	1076:1096	good biocompatibility	1076:1096	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	66	theme	good	1076:1079	arg1	efficacy					1134:1141	relatively high cellular uptake efficacy	1102:1141	relatively high cellular uptake efficacy	1102:1141	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	1	67	theme	photothermal	258:269	arg1	nanoagents					271:280	efficient photothermal nanoagents	248:280	efficient photothermal nanoagents for the therapy of tumor	248:305	CuS nanomaterials capped with artificial organic-molecules or polymers have been well demonstrated as efficient photothermal nanoagents for the therapy of tumor, but their biocompatibility and target ability should be improved.					
31401480	1	67	theme	photothermal	258:269	arg1	nanomaterials					150:162	CuS nanomaterials	146:162	CuS nanomaterials capped with artificial organic-molecules or polymers	146:215	CuS nanomaterials capped with artificial organic-molecules or polymers have been well demonstrated as efficient photothermal nanoagents for the therapy of tumor, but their biocompatibility and target ability should be improved.					
31401480	0	68	theme	dots	77:80	arg1	synthesis					24:32	Chitosan-mediated green synthesis	0:32	Chitosan-mediated green synthesis	0:32	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	0	68	theme	dots	77:80	arg1	modification					49:60	folic-acid modification	38:60	folic-acid modification	38:60	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	5	69	theme	QDs	790:792	arg1	composition					771:781	composition	771:781	composition	771:781	FA modification process has no apparent influence on the size, phase and composition of the QDs.					
31401480	5	69	theme	QDs	790:792	arg1	phase					761:765	phase	761:765	phase	761:765	FA modification process has no apparent influence on the size, phase and composition of the QDs.					
31401480	5	69	theme	QDs	790:792	arg1	size					755:758	size	755:758	size	755:758	FA modification process has no apparent influence on the size, phase and composition of the QDs.					
31401480	0	70	theme	quantum	69:75	arg1	dots					77:80	CuS quantum dots	65:80	CuS quantum dots	65:80	Chitosan-mediated green synthesis and folic-acid modification of CuS quantum dots for photoacoustic imaging guided photothermal therapy of tumor.					
31401480	6	71	theme	FA	890:891	arg1	conjugation					875:885	the efficient conjugation	861:885	the efficient conjugation of FA	861:891	Furthermore, the zeta potential and infrared spectroscopy confirm the efficient conjugation of FA.					
31401480	2	72	used	used	409:412	arg2	we					401:402	we	401:402	we	401:402	To address these problems, we have used chitosan (CS) as the biomacromolecule model and surface ligands to prepare CuS quantum dots (QDs) via a simple co-precipitation method.					
31401480	4	73	theme	phase	657:661	arg1	nanodots					663:670	hexagonal phase nanodots	647:670	hexagonal phase nanodots with sizes of about 4 nm	647:695	The resulting CuS-CS-FA QDs are composed of hexagonal phase nanodots with sizes of about 4 nm.					
31401480	8	74	theme	FA	1040:1041	arg1	modification					1043:1054	CS ligand and FA modification	1026:1054	modification	1043:1054	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	75	theme	high	1113:1116	arg1	result					1000:1005	a result	998:1005	a result of the presence of CS ligand and FA modification	998:1054	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	75	theme	high	1113:1116	arg1	biocompatibility					1081:1096	good biocompatibility	1076:1096	good biocompatibility	1076:1096	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
31401480	8	75	theme	high	1113:1116	arg1	efficacy					1134:1141	relatively high cellular uptake efficacy	1102:1141	relatively high cellular uptake efficacy	1102:1141	As a result of the presence of CS ligand and FA modification, CuS-CS-FA QDs have good biocompatibility and relatively high cellular uptake efficacy.					
30142905	3	0	theme	maltose	582:588	arg1	concentration					590:602	a maltose concentration	580:602	a maltose concentration of 7.4% (w/v)	580:616	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	6	1	with	treatment	1132:1140	arg1	lipopolysaccharide					1155:1172	1 μg/mL lipopolysaccharide	1147:1172	1 μg/mL lipopolysaccharide	1147:1172	However, nitric oxide production levels were similar to those following treatment with 1 μg/mL lipopolysaccharide.					
30142905	4	2	theme	column	862:867	arg1	chromatography					869:882	Bio-gel P2 gel permeation column chromatography	836:882	Bio-gel P2 gel permeation column chromatography	836:882	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	7	3	theme	inducible	1265:1273	arg1	synthase					1288:1295	inducible nitric oxide synthase	1265:1295	inducible nitric oxide synthase	1265:1295	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	0	4	theme	Surface	84:90	arg1	Methodology					92:102	a Response Surface Methodology	73:102	a Response Surface Methodology	73:102	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	6	5	theme	nitric	1069:1074	arg1	oxide					1076:1080	nitric oxide	1069:1080	nitric oxide production levels	1069:1098	However, nitric oxide production levels were similar to those following treatment with 1 μg/mL lipopolysaccharide.					
30142905	4	6	theme	permeation	851:860	arg1	chromatography					869:882	Bio-gel P2 gel permeation column chromatography	836:882	Bio-gel P2 gel permeation column chromatography	836:882	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	3	7	theme	sucrose	539:545	arg1	concentration					547:559	a sucrose concentration	537:559	a sucrose concentration of 9.6% (w/v)	537:573	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	8	8	theme	immune	1494:1499	arg1	enhancer					1501:1508	an immune enhancer	1491:1508	an immune enhancer of macrophages	1491:1523	These data suggest that oligosaccharides produced by Leuconostoc lactis CCK940 could be used as an immune enhancer of macrophages.					
30142905	8	8	theme	immune	1494:1499	arg1	oligosaccharides					1419:1434	oligosaccharides	1419:1434	oligosaccharides produced by Leuconostoc lactis CCK940	1419:1472	These data suggest that oligosaccharides produced by Leuconostoc lactis CCK940 could be used as an immune enhancer of macrophages.					
30142905	0	9	from	Optimization	0:11	arg1	Leuconostoc					48:58	Leuconostoc	48:58	Leuconostoc	48:58	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	2	10	used	used	402:405	arg2	concentrations					359:372	the concentrations	355:372	the concentrations of sucrose and maltose	355:395	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	10	used	used	402:405	arg2	temperature					339:349	Culture temperature	331:349	Culture temperature	331:349	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	10	used	used	402:405	arg2	factors					419:425	the main factors	410:425	the main factors	410:425	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	11	theme	Culture	331:337	arg1	factors					419:425	the main factors	410:425	the main factors	410:425	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	11	theme	Culture	331:337	arg1	temperature					339:349	Culture temperature	331:349	Culture temperature	331:349	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	11	theme	Culture	331:337	arg1	concentrations					359:372	the concentrations	355:372	the concentrations of sucrose and maltose	355:395	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	7	12	theme	interleukin-1β	1230:1243	arg1	levels					1195:1200	The mRNA expression levels	1175:1200	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase	1175:1295	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	7	13	theme	dose-dependent	1326:1339	arg1	manner					1341:1346	a dose-dependent manner	1324:1346	a dose-dependent manner	1324:1346	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	4	14	theme	resulting	745:753	arg1	oligosaccharides					755:770	the resulting oligosaccharides	741:770	the resulting oligosaccharides with a degree of polymerization greater than 4	741:817	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	1	15	theme	central	305:311	arg1	design					323:328	a central composite design	303:328	a central composite design	303:328	Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design.					
30142905	7	16	theme	necrosis	1211:1218	arg1	factor-α					1220:1227	tumor necrosis factor-α	1205:1227	tumor necrosis factor-α	1205:1227	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	3	17	theme	°C	533:534	arg1	conditions					450:459	The predicted optimum conditions	428:459	The predicted optimum conditions for the production of oligosaccharides	428:498	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	17	theme	°C	533:534	arg1	concentration					547:559	a sucrose concentration	537:559	a sucrose concentration of 9.6% (w/v)	537:573	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	17	theme	°C	533:534	arg1	concentration					590:602	a maltose concentration	580:602	a maltose concentration of 7.4% (w/v)	580:616	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	17	theme	°C	533:534	arg1	temperature					515:525	a culture temperature	505:525	a culture temperature of 30 °C	505:534	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	4	18	theme	Bio-gel	836:842	arg1	chromatography					869:882	Bio-gel P2 gel permeation column chromatography	836:882	Bio-gel P2 gel permeation column chromatography	836:882	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	6	19	theme	production	1082:1091	arg1	levels					1093:1098	nitric oxide production levels	1069:1098	nitric oxide production levels	1069:1098	However, nitric oxide production levels were similar to those following treatment with 1 μg/mL lipopolysaccharide.					
30142905	0	20	theme	Immunostimulating	112:128	arg1	Effects					130:136	the Immunostimulating Effects	108:136	the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells	108:182	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	0	21	from	Leuconostoc	48:58	arg1	Optimization					0:11	Optimization	0:11	Optimization of Oligosaccharide Production from Leuconostoc lactis	0:65	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	0	21	from	Leuconostoc	48:58	arg1	Production					32:41	Oligosaccharide Production	16:41	Oligosaccharide Production from Leuconostoc lactis	16:65	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	3	22	theme	%	567:567	arg1	conditions					450:459	The predicted optimum conditions	428:459	The predicted optimum conditions for the production of oligosaccharides	428:498	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	22	theme	%	567:567	arg1	concentration					547:559	a sucrose concentration	537:559	a sucrose concentration of 9.6% (w/v)	537:573	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	22	theme	%	567:567	arg1	concentration					590:602	a maltose concentration	580:602	a maltose concentration of 7.4% (w/v)	580:616	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	22	theme	%	567:567	arg1	temperature					515:525	a culture temperature	505:525	a culture temperature of 30 °C	505:534	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	1	23	theme	oligosaccharides	199:214	arg1	Production					185:194	Production	185:194	Production of oligosaccharides from Leuconostoc lactis CCK940	185:245	Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design.					
30142905	7	24	theme	interleukin-6	1246:1258	arg1	levels					1195:1200	The mRNA expression levels	1175:1200	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase	1175:1295	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	4	25	theme	gel	847:849	arg1	chromatography					869:882	Bio-gel P2 gel permeation column chromatography	836:882	Bio-gel P2 gel permeation column chromatography	836:882	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	7	26	with	treatment	1358:1366	arg1	oligosaccharides					1377:1392	the oligosaccharides	1373:1392	the oligosaccharides	1373:1392	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	4	27	theme	lactis	663:668	arg1	CCK940					670:675	Leuconostoc lactis CCK940	651:675	Leuconostoc lactis CCK940	651:675	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	7	28	theme	tumor	1205:1209	arg1	factor-α					1220:1227	tumor necrosis factor-α	1205:1227	tumor necrosis factor-α	1205:1227	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	1	29	theme	composite	313:321	arg1	design					323:328	a central composite design	303:328	a central composite design	303:328	Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design.					
30142905	4	30	theme	P2	844:845	arg1	chromatography					869:882	Bio-gel P2 gel permeation column chromatography	836:882	Bio-gel P2 gel permeation column chromatography	836:882	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	5	31	theme	purified	945:952	arg1	oligosaccharides					954:969	the purified oligosaccharides	941:969	the purified oligosaccharides	941:969	When macrophages were treated with the purified oligosaccharides at concentrations of 0.1⁻10 mg/mL, no cytotoxicity towards the macrophages was observed.					
30142905	2	32	theme	main	414:417	arg1	factors					419:425	the main factors	410:425	the main factors	410:425	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	32	theme	main	414:417	arg1	temperature					339:349	Culture temperature	331:349	Culture temperature	331:349	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	32	theme	main	414:417	arg1	concentrations					359:372	the concentrations	355:372	the concentrations of sucrose and maltose	355:395	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	0	33	theme	Production	32:41	arg1	Optimization					0:11	Optimization	0:11	Optimization of Oligosaccharide Production from Leuconostoc lactis	0:65	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	3	34	theme	culture	507:513	arg1	conditions					450:459	The predicted optimum conditions	428:459	The predicted optimum conditions for the production of oligosaccharides	428:498	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	34	theme	culture	507:513	arg1	temperature					515:525	a culture temperature	505:525	a culture temperature of 30 °C	505:534	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	0	35	theme	Oligosaccharide	16:30	arg1	Production					32:41	Oligosaccharide Production	16:41	Oligosaccharide Production from Leuconostoc lactis	16:65	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	4	36	with	oligosaccharides	755:770	arg1	degree					779:784	a degree	777:784	a degree of polymerization greater than 4	777:817	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	0	37	from	Methodology	92:102	arg1	Cells					178:182	Macrophage Cells	167:182	Macrophage Cells	167:182	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	7	38	theme	nitric	1275:1280	arg1	synthase					1288:1295	inducible nitric oxide synthase	1265:1295	inducible nitric oxide synthase	1265:1295	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	8	39	used	used	1483:1486	arg2	oligosaccharides					1419:1434	oligosaccharides	1419:1434	oligosaccharides produced by Leuconostoc lactis CCK940	1419:1472	These data suggest that oligosaccharides produced by Leuconostoc lactis CCK940 could be used as an immune enhancer of macrophages.					
30142905	8	39	used	used	1483:1486	arg2	enhancer					1501:1508	an immune enhancer	1491:1508	an immune enhancer of macrophages	1491:1523	These data suggest that oligosaccharides produced by Leuconostoc lactis CCK940 could be used as an immune enhancer of macrophages.					
30142905	8	40	theme	Leuconostoc	1448:1458	arg1	lactis					1460:1465	Leuconostoc lactis CCK940	1448:1472	Leuconostoc lactis CCK940	1448:1472	These data suggest that oligosaccharides produced by Leuconostoc lactis CCK940 could be used as an immune enhancer of macrophages.					
30142905	4	41	theme	Leuconostoc	651:661	arg1	CCK940					670:675	Leuconostoc lactis CCK940	651:675	Leuconostoc lactis CCK940	651:675	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	4	42	theme	optimal	631:637	arg1	conditions					639:648	these optimal conditions	625:648	these optimal conditions	625:648	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	7	43	theme	mRNA	1179:1182	arg1	levels					1195:1200	The mRNA expression levels	1175:1200	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase	1175:1295	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	0	44	theme	Oligosaccharides	147:162	arg1	Methodology					92:102	a Response Surface Methodology	73:102	a Response Surface Methodology	73:102	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	0	44	theme	Oligosaccharides	147:162	arg1	Effects					130:136	the Immunostimulating Effects	108:136	the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells	108:182	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	1	45	dep	Leuconostoc	221:231	arg1	CCK940					240:245	Leuconostoc lactis CCK940	221:245	Leuconostoc lactis CCK940	221:245	Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design.					
30142905	7	46	theme	factor-α	1220:1227	arg1	levels					1195:1200	The mRNA expression levels	1175:1200	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase	1175:1295	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	3	47	theme	predicted	432:440	arg1	conditions					450:459	The predicted optimum conditions	428:459	The predicted optimum conditions for the production of oligosaccharides	428:498	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	47	theme	predicted	432:440	arg1	temperature					515:525	a culture temperature	505:525	a culture temperature of 30 °C	505:534	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	0	48	dep	Leuconostoc	48:58	arg1	lactis					60:65	Leuconostoc lactis	48:65	Leuconostoc lactis	48:65	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	2	49	theme	maltose	389:395	arg1	factors					419:425	the main factors	410:425	the main factors	410:425	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	49	theme	maltose	389:395	arg1	temperature					339:349	Culture temperature	331:349	Culture temperature	331:349	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	49	theme	maltose	389:395	arg1	concentrations					359:372	the concentrations	355:372	the concentrations of sucrose and maltose	355:395	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	3	50	theme	optimum	442:448	arg1	conditions					450:459	The predicted optimum conditions	428:459	The predicted optimum conditions for the production of oligosaccharides	428:498	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	50	theme	optimum	442:448	arg1	temperature					515:525	a culture temperature	505:525	a culture temperature of 30 °C	505:534	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	0	51	theme	Macrophage	167:176	arg1	Cells					178:182	Macrophage Cells	167:182	Macrophage Cells	167:182	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	0	52	from	Effects	130:136	arg1	Cells					178:182	Macrophage Cells	167:182	Macrophage Cells	167:182	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	7	53	theme	expression	1184:1193	arg1	levels					1195:1200	The mRNA expression levels	1175:1200	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase	1175:1295	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	8	54	theme	macrophages	1513:1523	arg1	enhancer					1501:1508	an immune enhancer	1491:1508	an immune enhancer of macrophages	1491:1523	These data suggest that oligosaccharides produced by Leuconostoc lactis CCK940 could be used as an immune enhancer of macrophages.					
30142905	8	54	theme	macrophages	1513:1523	arg1	oligosaccharides					1419:1434	oligosaccharides	1419:1434	oligosaccharides produced by Leuconostoc lactis CCK940	1419:1472	These data suggest that oligosaccharides produced by Leuconostoc lactis CCK940 could be used as an immune enhancer of macrophages.					
30142905	1	55	from	Production	185:194	arg1	Leuconostoc					221:231	Leuconostoc	221:231	Leuconostoc	221:231	Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design.					
30142905	2	56	theme	sucrose	377:383	arg1	factors					419:425	the main factors	410:425	the main factors	410:425	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	56	theme	sucrose	377:383	arg1	temperature					339:349	Culture temperature	331:349	Culture temperature	331:349	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	2	56	theme	sucrose	377:383	arg1	concentrations					359:372	the concentrations	355:372	the concentrations of sucrose and maltose	355:395	Culture temperature and the concentrations of sucrose and maltose were used as the main factors.					
30142905	5	57	theme	mg/mL	999:1003	arg1	concentrations					974:987	concentrations	974:987	concentrations of 0.1⁻10 mg/mL	974:1003	When macrophages were treated with the purified oligosaccharides at concentrations of 0.1⁻10 mg/mL, no cytotoxicity towards the macrophages was observed.					
30142905	6	58	theme	oxide	1076:1080	arg1	production					1082:1091	nitric oxide production	1069:1091	nitric oxide production levels	1069:1098	However, nitric oxide production levels were similar to those following treatment with 1 μg/mL lipopolysaccharide.					
30142905	6	59	theme	μg/mL	1149:1153	arg1	lipopolysaccharide					1155:1172	1 μg/mL lipopolysaccharide	1147:1172	1 μg/mL lipopolysaccharide	1147:1172	However, nitric oxide production levels were similar to those following treatment with 1 μg/mL lipopolysaccharide.					
30142905	0	60	theme	Response	75:82	arg1	Methodology					92:102	a Response Surface Methodology	73:102	a Response Surface Methodology	73:102	Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells.					
30142905	3	61	theme	%	610:610	arg1	conditions					450:459	The predicted optimum conditions	428:459	The predicted optimum conditions for the production of oligosaccharides	428:498	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	61	theme	%	610:610	arg1	concentration					547:559	a sucrose concentration	537:559	a sucrose concentration of 9.6% (w/v)	537:573	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	61	theme	%	610:610	arg1	concentration					590:602	a maltose concentration	580:602	a maltose concentration of 7.4% (w/v)	580:616	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	3	61	theme	%	610:610	arg1	temperature					515:525	a culture temperature	505:525	a culture temperature of 30 °C	505:534	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	1	62	from	Leuconostoc	221:231	arg1	oligosaccharides					199:214	oligosaccharides	199:214	oligosaccharides from Leuconostoc lactis CCK940	199:245	Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design.					
30142905	1	62	from	Leuconostoc	221:231	arg1	Production					185:194	Production	185:194	Production of oligosaccharides from Leuconostoc lactis CCK940	185:245	Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design.					
30142905	7	63	theme	oxide	1282:1286	arg1	synthase					1288:1295	inducible nitric oxide synthase	1265:1295	inducible nitric oxide synthase	1265:1295	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	3	64	theme	oligosaccharides	483:498	arg1	production					469:478	the production	465:478	the production of oligosaccharides	465:498	The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 °C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v).					
30142905	1	65	theme	response	269:276	arg1	methodology					286:296	a response surface methodology	267:296	a response surface methodology	267:296	Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design.					
30142905	4	66	theme	polymerization	789:802	arg1	degree					779:784	a degree	777:784	a degree of polymerization greater than 4	777:817	Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized.					
30142905	7	67	theme	synthase	1288:1295	arg1	levels					1195:1200	The mRNA expression levels	1175:1200	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase	1175:1295	The mRNA expression levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides.					
30142905	1	68	theme	surface	278:284	arg1	methodology					286:296	a response surface methodology	267:296	a response surface methodology	267:296	Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design.					
29742511	12	0	theme	effective	1682:1690	arg1	procedure					1711:1719	an effective and time-efficient procedure	1679:1719	an effective and time-efficient procedure	1679:1719	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	0	theme	effective	1682:1690	arg1	method					1671:1676	the detergent-based method	1651:1676	the detergent-based method	1651:1676	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	1	theme	engineering-based	1844:1860	arg1	studies					1862:1868	pancreas tissue engineering-based studies	1828:1868	pancreas tissue engineering-based studies	1828:1868	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	8	2	theme	0.05	1089:1092	arg1	%					1093:1093	%	1093:1093	%	1093:1093	In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics.					
29742511	7	3	theme	sodium	915:920	arg1	sulfate					930:936	sodium dodecyl sulfate	915:936	sodium dodecyl sulfate (SDS)	915:942	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	7	3	theme	sodium	915:920	arg1	SDS					939:941	SDS	939:941	SDS	939:941	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	4	4	theme	organ	609:613	arg1	nature					590:595	the compositional, architectural, and biomechanical nature	538:595	the compositional, architectural, and biomechanical nature of a native organ	538:613	The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ.					
29742511	0	5	theme	Arterial	80:87	arg1	Catheterization					89:103	Ductal or Arterial Catheterization	70:103	Ductal or Arterial Catheterization	70:103	Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization.					
29742511	4	6	theme	compositional	542:554	arg1	nature					590:595	the compositional, architectural, and biomechanical nature	538:595	the compositional, architectural, and biomechanical nature of a native organ	538:613	The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ.					
29742511	4	7	theme	perfusion-decellularization	444:470	arg1	technique					472:480	The perfusion-decellularization technique	440:480	The perfusion-decellularization technique	440:480	The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ.					
29742511	8	8	with	bioscaffold	1109:1119	arg1	characteristics					1181:1195	proper biomechanical characteristics	1160:1195	proper biomechanical characteristics	1160:1195	In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics.					
29742511	8	8	with	bioscaffold	1109:1119	arg1	matrices					1147:1154	intact extracellular matrices	1126:1154	intact extracellular matrices	1126:1154	In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics.					
29742511	1	9	theme	great	167:171	arg1	burden					173:178	a great burden	165:178	a great burden on society	165:189	INTRODUCTION Diabetes is known as a worldwide disease with a great burden on society.					
29742511	7	10	theme	High	892:895	arg1	concentrations					897:910	High concentrations	892:910	High concentrations	892:910	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	4	11	theme	native	602:607	arg1	organ					609:613	a native organ	600:613	a native organ	600:613	The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ.					
29742511	3	12	theme	islet	364:368	arg1	cells					370:374	islet cells	364:374	islet cells	364:374	Bioscaffolds along with islet cells would provide bioengineered tissue as a substitute for β-cells.					
29742511	10	13	theme	native	1436:1441	arg1	tissue					1452:1457	native pancreas tissue	1436:1457	native pancreas tissue	1436:1457	Quantitative findings showed that glycosaminoglycan content was slightly different, but hydroxyproline was in the range of native pancreas tissue.					
29742511	1	14	with	disease	152:158	arg1	burden					173:178	a great burden	165:178	a great burden on society	165:189	INTRODUCTION Diabetes is known as a worldwide disease with a great burden on society.					
29742511	9	15	theme	collagen	1262:1269	arg1	concentrations					1277:1290	collagen fiber concentrations	1262:1290	collagen fiber concentrations	1262:1290	Tissue-specific stainings revealed that elastic, reticular, and collagen fiber concentrations were well preserved.					
29742511	12	16	theme	tissue	1837:1842	arg1	studies					1862:1868	pancreas tissue engineering-based studies	1828:1868	pancreas tissue engineering-based studies	1828:1868	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	11	17	theme	homogeneous	1603:1613	arg1	structure					1622:1630	a homogeneous porous structure	1601:1630	a homogeneous porous structure	1601:1630	Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure.					
29742511	4	18	theme	such	506:509	arg1	scaffolds					511:519	such scaffolds	506:519	such scaffolds	506:519	The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ.					
29742511	10	19	theme	tissue	1452:1457	arg1	range					1427:1431	the range	1423:1431	the range of native pancreas tissue	1423:1457	Quantitative findings showed that glycosaminoglycan content was slightly different, but hydroxyproline was in the range of native pancreas tissue.					
29742511	11	20	theme	Dye	1460:1462	arg1	infusion					1464:1471	Dye infusion	1460:1471	Dye infusion through ductal and vascular cannulation	1460:1511	Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure.					
29742511	12	21	theme	extracellular	1739:1751	arg1	matrix					1753:1758	a whole pancreas extracellular matrix	1722:1758	a whole pancreas extracellular matrix	1722:1758	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	21	theme	extracellular	1739:1751	arg1	method					1671:1676	the detergent-based method	1651:1676	the detergent-based method	1651:1676	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	6	22	dep	pancreas	729:736	arg1	rats					758:761	Sprague-Dawley rats	743:761	Sprague-Dawley rats	743:761	METHODS Procured pancreas from Sprague-Dawley rats was exposed to different percentages of detergent for 2, 4, and 6 h after cannulation via the common bile duct or aorta.					
29742511	7	23	dep	%	957:957	arg1	i.e.					945:948	i.e.	945:948	i.e.	945:948	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	8	24	theme	extracellular	1133:1145	arg1	matrices					1147:1154	intact extracellular matrices	1126:1154	intact extracellular matrices	1126:1154	In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics.					
29742511	3	25	dep	a	414:414	arg1	substitute					416:425	substitute	416:425	substitute for β-cells	416:437	Bioscaffolds along with islet cells would provide bioengineered tissue as a substitute for β-cells.					
29742511	10	26	theme	pancreas	1443:1450	arg1	tissue					1452:1457	native pancreas tissue	1436:1457	native pancreas tissue	1436:1457	Quantitative findings showed that glycosaminoglycan content was slightly different, but hydroxyproline was in the range of native pancreas tissue.					
29742511	2	27	theme	therapeutic	263:273	arg1	strategies					275:284	new therapeutic strategies	259:284	new therapeutic strategies in the field of regenerative medicine	259:322	Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered.					
29742511	0	28	theme	Pancreas	15:22	arg1	Scaffolds					31:39	Pancreas Matrix Scaffolds	15:39	Pancreas Matrix Scaffolds	15:39	Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization.					
29742511	5	29	theme	decellularization	649:665	arg1	methods					667:673	2 decellularization methods	647:673	2 decellularization methods preserving tissue microarchitecture	647:709	In this study, we investigated 2 decellularization methods preserving tissue microarchitecture.					
29742511	9	30	theme	Tissue-specific	1198:1212	arg1	stainings					1214:1222	Tissue-specific stainings	1198:1222	Tissue-specific stainings	1198:1222	Tissue-specific stainings revealed that elastic, reticular, and collagen fiber concentrations were well preserved.					
29742511	2	31	theme	medicine	315:322	arg1	field					293:297	the field	289:297	the field of regenerative medicine	289:322	Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered.					
29742511	6	32	theme	Sprague-Dawley	743:756	arg1	rats					758:761	Sprague-Dawley rats	743:761	Sprague-Dawley rats	743:761	METHODS Procured pancreas from Sprague-Dawley rats was exposed to different percentages of detergent for 2, 4, and 6 h after cannulation via the common bile duct or aorta.					
29742511	2	33	theme	regenerative	302:313	arg1	medicine					315:322	regenerative medicine	302:322	regenerative medicine	302:322	Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered.					
29742511	5	34	theme	tissue	686:691	arg1	microarchitecture					693:709	tissue microarchitecture	686:709	tissue microarchitecture	686:709	In this study, we investigated 2 decellularization methods preserving tissue microarchitecture.					
29742511	4	35	theme	biomechanical	576:588	arg1	nature					590:595	the compositional, architectural, and biomechanical nature	538:595	the compositional, architectural, and biomechanical nature of a native organ	538:613	The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ.					
29742511	11	36	theme	vascular	1492:1499	arg1	cannulation					1501:1511	ductal and vascular cannulation	1481:1511	ductal and vascular cannulation	1481:1511	Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure.					
29742511	12	37	theme	3D	1811:1812	arg1	that					1789:1792	that	1789:1792	that	1789:1792	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	37	theme	3D	1811:1812	arg1	structure					1814:1822	a 3D structure	1809:1822	a 3D structure for pancreas tissue engineering-based studies	1809:1868	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	11	38	theme	scanning	1562:1569	arg1	microscopy					1580:1589	scanning electron microscopy	1562:1589	scanning electron microscopy	1562:1589	Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure.					
29742511	1	39	theme	INTRODUCTION	106:117	arg1	Diabetes					119:126	INTRODUCTION Diabetes	106:126	INTRODUCTION Diabetes	106:126	INTRODUCTION Diabetes is known as a worldwide disease with a great burden on society.					
29742511	1	39	theme	INTRODUCTION	106:117	arg1	disease					152:158	a worldwide disease	140:158	a worldwide disease with a great burden on society	140:189	INTRODUCTION Diabetes is known as a worldwide disease with a great burden on society.					
29742511	11	40	theme	porous	1615:1620	arg1	structure					1622:1630	a homogeneous porous structure	1601:1630	a homogeneous porous structure	1601:1630	Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure.					
29742511	11	41	theme	ductal	1481:1486	arg1	cannulation					1501:1511	ductal and vascular cannulation	1481:1511	ductal and vascular cannulation	1481:1511	Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure.					
29742511	10	42	theme	Quantitative	1313:1324	arg1	findings					1326:1333	Quantitative findings	1313:1333	Quantitative findings	1313:1333	Quantitative findings showed that glycosaminoglycan content was slightly different, but hydroxyproline was in the range of native pancreas tissue.					
29742511	0	43	theme	Tissue	45:50	arg1	Engineering					52:62	Tissue Engineering	45:62	Tissue Engineering Using Ductal or Arterial Catheterization	45:103	Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization.					
29742511	12	44	theme	detergent-based	1655:1669	arg1	procedure					1711:1719	an effective and time-efficient procedure	1679:1719	an effective and time-efficient procedure	1679:1719	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	44	theme	detergent-based	1655:1669	arg1	matrix					1753:1758	a whole pancreas extracellular matrix	1722:1758	a whole pancreas extracellular matrix	1722:1758	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	44	theme	detergent-based	1655:1669	arg1	method					1671:1676	the detergent-based method	1651:1676	the detergent-based method	1651:1676	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	8	45	theme	6-h	1073:1075	arg1	exposure					1077:1084	6-h exposure	1073:1084	6-h exposure to 0.05% SDS	1073:1097	In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics.					
29742511	11	46	theme	vascular	1529:1536	arg1	network					1538:1544	the vascular network	1525:1544	the vascular network	1525:1544	Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure.					
29742511	11	46	theme	vascular	1529:1536	arg1	intact					1550:1555	intact	1550:1555	intact	1550:1555	Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure.					
29742511	8	47	theme	biomechanical	1167:1179	arg1	characteristics					1181:1195	proper biomechanical characteristics	1160:1195	proper biomechanical characteristics	1160:1195	In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics.					
29742511	11	48	theme	electron	1571:1578	arg1	microscopy					1580:1589	scanning electron microscopy	1562:1589	scanning electron microscopy	1562:1589	Dye infusion through ductal and vascular cannulation proved that the vascular network was intact, and scanning electron microscopy indicated a homogeneous porous structure.					
29742511	0	49	theme	Matrix	24:29	arg1	Scaffolds					31:39	Pancreas Matrix Scaffolds	15:39	Pancreas Matrix Scaffolds	15:39	Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization.					
29742511	4	50	theme	architectural	557:569	arg1	nature					590:595	the compositional, architectural, and biomechanical nature	538:595	the compositional, architectural, and biomechanical nature of a native organ	538:613	The perfusion-decellularization technique is considered to create such scaffolds since they mimic the compositional, architectural, and biomechanical nature of a native organ.					
29742511	7	51	theme	exposure	1046:1053	arg1	duration					1034:1041	the duration	1030:1041	the duration of exposure	1030:1053	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	12	52	theme	time-efficient	1696:1709	arg1	procedure					1711:1719	an effective and time-efficient procedure	1679:1719	an effective and time-efficient procedure	1679:1719	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	52	theme	time-efficient	1696:1709	arg1	method					1671:1676	the detergent-based method	1651:1676	the detergent-based method	1651:1676	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	7	53	theme	cell	1004:1007	arg1	removal					1009:1015	incomplete cell removal	993:1015	incomplete cell removal	993:1015	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	8	54	theme	proper	1160:1165	arg1	characteristics					1181:1195	proper biomechanical characteristics	1160:1195	proper biomechanical characteristics	1160:1195	In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics.					
29742511	7	55	dep	RESULTS	884:890	arg1	resulted					960:967	resulted	960:967	resulted in tissue disruption or incomplete cell removal depending on the duration of exposure	960:1053	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	12	56	theme	regenerative	1874:1885	arg1	that					1789:1792	that	1789:1792	that	1789:1792	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	56	theme	regenerative	1874:1885	arg1	medicine					1887:1894	regenerative medicine	1874:1894	regenerative medicine	1874:1894	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	2	57	theme	limited	226:232	arg1	number					234:239	a limited number	224:239	a limited number of target points	224:256	Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered.					
29742511	12	58	used	used	1801:1804	arg2	medicine					1887:1894	regenerative medicine	1874:1894	regenerative medicine	1874:1894	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	58	used	used	1801:1804	arg2	that					1789:1792	that	1789:1792	that	1789:1792	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	58	used	used	1801:1804	arg2	structure					1814:1822	a 3D structure	1809:1822	a 3D structure for pancreas tissue engineering-based studies	1809:1868	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	59	dep	structure	1814:1822	arg1	applications					1896:1907	applications	1896:1907	applications	1896:1907	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	10	60	theme	glycosaminoglycan	1347:1363	arg1	content					1365:1371	glycosaminoglycan content	1347:1371	glycosaminoglycan content	1347:1371	Quantitative findings showed that glycosaminoglycan content was slightly different, but hydroxyproline was in the range of native pancreas tissue.					
29742511	2	61	from	strategies	275:284	arg1	field					293:297	the field	289:297	the field of regenerative medicine	289:322	Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered.					
29742511	8	62	theme	%	1093:1093	arg1	SDS					1095:1097	0.05% SDS	1089:1097	0.05% SDS	1089:1097	In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics.					
29742511	6	63	theme	bile	864:867	arg1	duct					869:872	the common bile duct	853:872	the common bile duct	853:872	METHODS Procured pancreas from Sprague-Dawley rats was exposed to different percentages of detergent for 2, 4, and 6 h after cannulation via the common bile duct or aorta.					
29742511	2	64	theme	new	259:261	arg1	strategies					275:284	new therapeutic strategies	259:284	new therapeutic strategies in the field of regenerative medicine	259:322	Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered.					
29742511	6	65	theme	detergent	803:811	arg1	percentages					788:798	different percentages	778:798	different percentages of detergent	778:811	METHODS Procured pancreas from Sprague-Dawley rats was exposed to different percentages of detergent for 2, 4, and 6 h after cannulation via the common bile duct or aorta.					
29742511	12	66	theme	whole	1724:1728	arg1	pancreas					1730:1737	a whole pancreas	1722:1737	a whole pancreas extracellular matrix	1722:1758	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	6	67	theme	different	778:786	arg1	percentages					788:798	different percentages	778:798	different percentages of detergent	778:811	METHODS Procured pancreas from Sprague-Dawley rats was exposed to different percentages of detergent for 2, 4, and 6 h after cannulation via the common bile duct or aorta.					
29742511	8	68	theme	intact	1126:1131	arg1	matrices					1147:1154	intact extracellular matrices	1126:1154	intact extracellular matrices	1126:1154	In both methods, 6-h exposure to 0.05% SDS created a bioscaffold with intact extracellular matrices and proper biomechanical characteristics.					
29742511	7	69	theme	incomplete	993:1002	arg1	removal					1009:1015	incomplete cell removal	993:1015	incomplete cell removal	993:1015	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	2	70	theme	therapeutic	198:208	arg1	options					210:216	therapeutic options	198:216	therapeutic options	198:216	Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered.					
29742511	7	71	theme	tissue	972:977	arg1	disruption					979:988	tissue disruption	972:988	tissue disruption	972:988	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	1	72	theme	worldwide	142:150	arg1	Diabetes					119:126	INTRODUCTION Diabetes	106:126	INTRODUCTION Diabetes	106:126	INTRODUCTION Diabetes is known as a worldwide disease with a great burden on society.					
29742511	1	72	theme	worldwide	142:150	arg1	disease					152:158	a worldwide disease	140:158	a worldwide disease with a great burden on society	140:189	INTRODUCTION Diabetes is known as a worldwide disease with a great burden on society.					
29742511	7	73	theme	dodecyl	922:928	arg1	sulfate					930:936	sodium dodecyl sulfate	915:936	sodium dodecyl sulfate (SDS)	915:942	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	7	73	theme	dodecyl	922:928	arg1	SDS					939:941	SDS	939:941	SDS	939:941	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	12	74	theme	pancreas	1828:1835	arg1	studies					1862:1868	pancreas tissue engineering-based studies	1828:1868	pancreas tissue engineering-based studies	1828:1868	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	9	75	theme	fiber	1271:1275	arg1	concentrations					1277:1290	collagen fiber concentrations	1262:1290	collagen fiber concentrations	1262:1290	Tissue-specific stainings revealed that elastic, reticular, and collagen fiber concentrations were well preserved.					
29742511	2	76	theme	points	251:256	arg1	number					234:239	a limited number	224:239	a limited number of target points	224:256	Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered.					
29742511	6	77	theme	common	857:862	arg1	duct					869:872	the common bile duct	853:872	the common bile duct	853:872	METHODS Procured pancreas from Sprague-Dawley rats was exposed to different percentages of detergent for 2, 4, and 6 h after cannulation via the common bile duct or aorta.					
29742511	7	78	theme	sulfate	930:936	arg1	concentrations					897:910	High concentrations	892:910	High concentrations	892:910	RESULTS High concentrations of sodium dodecyl sulfate (SDS), i.e., > 0.05%, resulted in tissue disruption or incomplete cell removal depending on the duration of exposure.					
29742511	0	79	theme	Ductal	70:75	arg1	Catheterization					89:103	Ductal or Arterial Catheterization	70:103	Ductal or Arterial Catheterization	70:103	Decellularized Pancreas Matrix Scaffolds for Tissue Engineering Using Ductal or Arterial Catheterization.					
29742511	2	80	theme	target	244:249	arg1	points					251:256	target points	244:256	target points	244:256	Since therapeutic options cover a limited number of target points, new therapeutic strategies in the field of regenerative medicine are considered.					
29742511	1	81	from	burden	173:178	arg1	society					183:189	society	183:189	society	183:189	INTRODUCTION Diabetes is known as a worldwide disease with a great burden on society.					
29742511	3	82	theme	bioengineered	390:402	arg1	tissue					404:409	bioengineered tissue	390:409	bioengineered tissue	390:409	Bioscaffolds along with islet cells would provide bioengineered tissue as a substitute for β-cells.					
29742511	12	83	theme	pancreas	1730:1737	arg1	matrix					1753:1758	a whole pancreas extracellular matrix	1722:1758	a whole pancreas extracellular matrix	1722:1758	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
29742511	12	83	theme	pancreas	1730:1737	arg1	method					1671:1676	the detergent-based method	1651:1676	the detergent-based method	1651:1676	CONCLUSIONS Using the detergent-based method, an effective and time-efficient procedure, a whole pancreas extracellular matrix bioscaffold can be developed that can be used as a 3D structure for pancreas tissue engineering-based studies and regenerative medicine applications.					
30827590	0	0	from	lipolysis	12:20	arg1	cheese					62:67	raw sheep milk Tulum cheese	41:67	raw sheep milk Tulum cheese	41:67	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	1	1	from	variations	146:155	arg1	acids					196:200	organic acids	188:200	organic acids (OAs)	188:206	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	1	from	variations	146:155	arg1	acids					248:252	free fatty acids	237:252	free fatty acids	237:252	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	1	from	variations	146:155	arg1	chemical					166:173	chemical	166:173	chemical	166:173	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	1	from	variations	146:155	arg1	acids					220:224	free amino acids	209:224	free amino acids (FAAs)	209:231	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	5	2	theme	propionic	636:644	arg1	acid					646:649	propionic acid	636:649	propionic acid	636:649	Lactic acid was principal organic acid, followed by propionic acid, acetic acid, formic acid, succinic acid and citric acid.					
30827590	1	3	theme	free	209:212	arg1	FAAs					227:230	FAAs	227:230	FAAs	227:230	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	3	theme	free	209:212	arg1	chemical					166:173	chemical	166:173	chemical	166:173	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	3	theme	free	209:212	arg1	acids					220:224	free amino acids	209:224	free amino acids (FAAs)	209:231	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	0	4	from	Glycolysis	0:9	arg1	cheese					62:67	raw sheep milk Tulum cheese	41:67	raw sheep milk Tulum cheese	41:67	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	2	5	theme	cheese	460:465	arg1	compositions					438:449	chemical compositions	429:449	chemical compositions of Tulum cheese	429:465	Ripening in goat's skin caused the differences in chemical compositions of Tulum cheese.					
30827590	1	6	theme	amino	214:218	arg1	FAAs					227:230	FAAs	227:230	FAAs	227:230	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	6	theme	amino	214:218	arg1	chemical					166:173	chemical	166:173	chemical	166:173	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	6	theme	amino	214:218	arg1	acids					220:224	free amino acids	209:224	free amino acids (FAAs)	209:231	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	5	7	theme	principal	600:608	arg1	acid					618:621	principal organic acid	600:621	principal organic acid	600:621	Lactic acid was principal organic acid, followed by propionic acid, acetic acid, formic acid, succinic acid and citric acid.					
30827590	2	8	theme	Tulum	454:458	arg1	cheese					460:465	Tulum cheese	454:465	Tulum cheese	454:465	Ripening in goat's skin caused the differences in chemical compositions of Tulum cheese.					
30827590	5	9	theme	organic	610:616	arg1	acid					618:621	principal organic acid	600:621	principal organic acid	600:621	Lactic acid was principal organic acid, followed by propionic acid, acetic acid, formic acid, succinic acid and citric acid.					
30827590	1	10	theme	raw	280:282	arg1	cheeses					303:309	raw sheep's milk Tulum cheeses	280:309	raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT)	280:358	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	8	11	theme	total	1010:1014	arg1	OA					1016:1017	total OA	1010:1017	total OA	1010:1017	The levels of total FFA and total OA were higher in GST-cheese in comparison to PBT-cheese but total FAAs were lower.					
30827590	8	12	theme	total	996:1000	arg1	FFA					1002:1004	total FFA	996:1004	total FFA	996:1004	The levels of total FFA and total OA were higher in GST-cheese in comparison to PBT-cheese but total FAAs were lower.					
30827590	6	13	theme	amino	725:729	arg1	acids					731:735	amino acids	725:735	amino acids	725:735	The majority of amino acids were composed of Val, Pro, Tyr, Met, Leu, Trp, Phe, Lys and Glu.					
30827590	1	14	theme	plastic	339:345	arg1	barrel					347:352	plastic barrel	339:352	plastic barrel (PBT)	339:358	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	14	theme	plastic	339:345	arg1	PBT					355:357	PBT	355:357	PBT	355:357	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	7	15	theme	trans-9-octadecenoic	954:973	arg1	acids					975:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	7	16	theme	decanoic	941:948	arg1	acids					975:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	0	17	from	proteolysis	26:36	arg1	cheese					62:67	raw sheep milk Tulum cheese	41:67	raw sheep milk Tulum cheese	41:67	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	7	18	from	acid	853:856	arg1	cheeses					865:871	the cheeses	861:871	the cheeses	861:871	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	6	19	theme	acids	731:735	arg1	majority					713:720	The majority	709:720	The majority of amino acids	709:735	The majority of amino acids were composed of Val, Pro, Tyr, Met, Leu, Trp, Phe, Lys and Glu.					
30827590	4	20	theme	residual	540:547	arg1	lactose					549:555	No residual lactose	537:555	No residual lactose	537:555	No residual lactose was observed after 90 day.					
30827590	5	21	theme	formic	665:670	arg1	acid					672:675	formic acid	665:675	formic acid	665:675	Lactic acid was principal organic acid, followed by propionic acid, acetic acid, formic acid, succinic acid and citric acid.					
30827590	5	22	theme	Lactic	584:589	arg1	acid					591:594	Lactic acid	584:594	Lactic acid	584:594	Lactic acid was principal organic acid, followed by propionic acid, acetic acid, formic acid, succinic acid and citric acid.					
30827590	3	23	theme	protein	509:515	arg1	contents					517:524	protein contents	509:524	protein contents	509:524	Moisture loss, salt-in-moisture, fat and protein contents increased.					
30827590	7	24	theme	tetradecanoic	926:938	arg1	acids					975:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	7	25	theme	free	842:845	arg1	acid					853:856	the most abundant free fatty acid	824:856	the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	824:979	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	7	25	theme	free	842:845	arg1	acid					815:818	Hexadecanoic acid	802:818	Hexadecanoic acid	802:818	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	5	26	theme	acetic	652:657	arg1	acid					659:662	acetic acid	652:662	acetic acid	652:662	Lactic acid was principal organic acid, followed by propionic acid, acetic acid, formic acid, succinic acid and citric acid.					
30827590	5	27	theme	succinic	678:685	arg1	acid					687:690	succinic acid	678:690	succinic acid	678:690	Lactic acid was principal organic acid, followed by propionic acid, acetic acid, formic acid, succinic acid and citric acid.					
30827590	7	28	theme	fatty	847:851	arg1	acid					853:856	the most abundant free fatty acid	824:856	the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	824:979	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	7	28	theme	fatty	847:851	arg1	acid					815:818	Hexadecanoic acid	802:818	Hexadecanoic acid	802:818	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	1	29	theme	milk	292:295	arg1	cheeses					303:309	raw sheep's milk Tulum cheeses	280:309	raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT)	280:358	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	7	30	theme	octadecanoic	912:923	arg1	acids					975:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	0	31	theme	materials	120:128	arg1	Effect					101:106	Effect	101:106	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.	0:129	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	1	32	theme	Tulum	297:301	arg1	cheeses					303:309	raw sheep's milk Tulum cheeses	280:309	raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT)	280:358	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	8	33	theme	OA	1016:1017	arg1	higher					1024:1029	higher	1024:1029	higher	1024:1029	The levels of total FFA and total OA were higher in GST-cheese in comparison to PBT-cheese but total FAAs were lower.					
30827590	8	33	theme	OA	1016:1017	arg1	levels					986:991	The levels	982:991	The levels of total FFA and total OA	982:1017	The levels of total FFA and total OA were higher in GST-cheese in comparison to PBT-cheese but total FAAs were lower.					
30827590	7	34	theme	cis-9-octadecenoic	892:909	arg1	acids					975:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	892:979	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	1	35	theme	free	237:240	arg1	acids					248:252	free fatty acids	237:252	free fatty acids	237:252	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	35	theme	free	237:240	arg1	chemical					166:173	chemical	166:173	chemical	166:173	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	0	36	theme	ripening	111:118	arg1	materials					120:128	ripening materials	111:128	ripening materials	111:128	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	3	37	theme	Moisture	468:475	arg1	loss					477:480	Moisture loss	468:480	Moisture loss	468:480	Moisture loss, salt-in-moisture, fat and protein contents increased.					
30827590	1	38	theme	organic	188:194	arg1	OAs					203:205	OAs	203:205	OAs	203:205	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	38	theme	organic	188:194	arg1	acids					196:200	organic acids	188:200	organic acids (OAs)	188:206	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	38	theme	organic	188:194	arg1	FFAs					255:258	FFAs	255:258	FFAs	255:258	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	38	theme	organic	188:194	arg1	chemical					166:173	chemical	166:173	chemical	166:173	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	0	39	theme	sheep	45:49	arg1	milk					51:54	raw sheep milk	41:54	raw sheep milk Tulum cheese	41:67	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	1	40	theme	fatty	242:246	arg1	acids					248:252	free fatty acids	237:252	free fatty acids	237:252	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	40	theme	fatty	242:246	arg1	chemical					166:173	chemical	166:173	chemical	166:173	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	7	41	theme	Hexadecanoic	802:813	arg1	acid					853:856	the most abundant free fatty acid	824:856	the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	824:979	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	7	41	theme	Hexadecanoic	802:813	arg1	acid					815:818	Hexadecanoic acid	802:818	Hexadecanoic acid	802:818	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	7	42	theme	abundant	833:840	arg1	acid					853:856	the most abundant free fatty acid	824:856	the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids	824:979	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	7	42	theme	abundant	833:840	arg1	acid					815:818	Hexadecanoic acid	802:818	Hexadecanoic acid	802:818	Hexadecanoic acid was the most abundant free fatty acid in the cheeses, which followed by cis-9-octadecenoic, octadecanoic, tetradecanoic, decanoic and trans-9-octadecenoic acids.					
30827590	0	43	theme	raw	41:43	arg1	milk					51:54	raw sheep milk	41:54	raw sheep milk Tulum cheese	41:67	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	2	44	theme	chemical	429:436	arg1	compositions					438:449	chemical compositions	429:449	chemical compositions of Tulum cheese	429:465	Ripening in goat's skin caused the differences in chemical compositions of Tulum cheese.					
30827590	2	45	from	Ripening	379:386	arg1	skin					398:401	goat's skin	391:401	goat's skin	391:401	Ripening in goat's skin caused the differences in chemical compositions of Tulum cheese.					
30827590	1	46	theme	skin	321:324	arg1	GST					331:333	GST	331:333	GST	331:333	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	1	46	theme	skin	321:324	arg1	bag					326:328	goat's skin bag	314:328	goat's skin bag (GST)	314:334	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	8	47	theme	FFA	1002:1004	arg1	higher					1024:1029	higher	1024:1029	higher	1024:1029	The levels of total FFA and total OA were higher in GST-cheese in comparison to PBT-cheese but total FAAs were lower.					
30827590	8	47	theme	FFA	1002:1004	arg1	levels					986:991	The levels	982:991	The levels of total FFA and total OA	982:1017	The levels of total FFA and total OA were higher in GST-cheese in comparison to PBT-cheese but total FAAs were lower.					
30827590	0	48	theme	Tulum	56:60	arg1	cheese					62:67	raw sheep milk Tulum cheese	41:67	raw sheep milk Tulum cheese	41:67	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	5	49	theme	citric	696:701	arg1	acid					703:706	citric acid	696:706	citric acid	696:706	Lactic acid was principal organic acid, followed by propionic acid, acetic acid, formic acid, succinic acid and citric acid.					
30827590	1	50	dep	chemical	166:173	arg1	constitutes					175:185	constitutes	175:185	constitutes	175:185	In this study, variations in basic chemical constitutes, organic acids (OAs), free amino acids (FAAs) and free fatty acids (FFAs) during ripening of raw sheep's milk Tulum cheeses in goat's skin bag (GST) or plastic barrel (PBT) were investigated.					
30827590	2	51	from	differences	414:424	arg1	compositions					438:449	chemical compositions	429:449	chemical compositions of Tulum cheese	429:465	Ripening in goat's skin caused the differences in chemical compositions of Tulum cheese.					
30827590	0	52	theme	milk	51:54	arg1	cheese					62:67	raw sheep milk Tulum cheese	41:67	raw sheep milk Tulum cheese	41:67	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	0	53	dep	Glycolysis	0:9	arg1	Effect					101:106	Effect	101:106	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.	0:129	Glycolysis, lipolysis and proteolysis in raw sheep milk Tulum cheese during production and ripening: Effect of ripening materials.					
30827590	8	54	theme	total	1077:1081	arg1	FAAs					1083:1086	total FAAs	1077:1086	total FAAs	1077:1086	The levels of total FFA and total OA were higher in GST-cheese in comparison to PBT-cheese but total FAAs were lower.					
30755095	12	0	theme	histological	1497:1508	arg1	examination					1510:1520	histological examination	1497:1520	histological examination	1497:1520	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	6	1	theme	composite	713:721	arg1	dressing					723:730	the composite dressing	709:730	the composite dressing	709:730	The asymmetric wettability of the composite dressing was demonstrated by water contact angle measurement.					
30755095	1	2	theme	solution	126:133	arg1	scaffold					228:235	the scaffold	224:235	the scaffold	224:235	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	1	2	theme	solution	126:133	arg1	emulsion					152:159	a solution chitosan fibroin emulsion	124:159	a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs)	124:199	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	7	3	theme	physical	916:923	arg1	properties					940:949	the physical and mechanical properties	912:949	the physical and mechanical properties	912:949	Relatively high porosity, favourable moisture retention capability and appropriate tensile strength were observed by measuring the physical and mechanical properties.					
30755095	4	4	theme	electron	561:568	arg1	microscope					570:579	a scanning electron microscope	550:579	a scanning electron microscope	550:579	The morphologies of the composite dressing were examined under a scanning electron microscope.					
30755095	0	5	theme	moisturizing	57:68	arg1	evaluation					21:30	In vitro and in vivo evaluation	0:30	In vitro and in vivo evaluation of new PRP antibacterial moisturizing	0:68	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	1	6	theme	chitosan	135:142	arg1	scaffold					228:235	the scaffold	224:235	the scaffold	224:235	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	1	6	theme	chitosan	135:142	arg1	emulsion					152:159	a solution chitosan fibroin emulsion	124:159	a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs)	124:199	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	9	7	theme	protein	1122:1128	arg1	property					1143:1150	The total protein slow-release property	1112:1150	The total protein slow-release property	1112:1150	The total protein slow-release property was measured using the BCA assay.					
30755095	4	8	theme	scanning	552:559	arg1	microscope					570:579	a scanning electron microscope	550:579	a scanning electron microscope	550:579	The morphologies of the composite dressing were examined under a scanning electron microscope.					
30755095	0	9	theme	infectious	84:93	arg1	repair					101:106	infectious wound repair	84:106	infectious wound repair	84:106	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	5	10	theme	coupled	645:651	arg1	spectrometry					665:676	inductively coupled plasma mass spectrometry	633:676	inductively coupled plasma mass spectrometry	633:676	The silver content of the dressing was measured by inductively coupled plasma mass spectrometry.					
30755095	5	11	theme	silver	586:591	arg1	content					593:599	The silver content	582:599	The silver content of the dressing	582:615	The silver content of the dressing was measured by inductively coupled plasma mass spectrometry.					
30755095	12	12	theme	exudate	1458:1464	arg1	cultures					1440:1447	bacterial cultures	1430:1447	bacterial cultures of wound exudate	1430:1464	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	12	12	theme	exudate	1458:1464	arg1	examination					1510:1520	histological examination	1497:1520	histological examination	1497:1520	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	12	12	theme	exudate	1458:1464	arg1	Analysis					1397:1404	Analysis	1397:1404	Analysis of wound healing rates	1397:1427	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	12	12	theme	exudate	1458:1464	arg1	analysis					1484:1491	whole blood cell analysis	1467:1491	whole blood cell analysis	1467:1491	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	9	13	theme	slow-release	1130:1141	arg1	property					1143:1150	The total protein slow-release property	1112:1150	The total protein slow-release property	1112:1150	The total protein slow-release property was measured using the BCA assay.					
30755095	13	14	theme	wound	1641:1645	arg1	repair					1654:1659	wound tissue repair	1641:1659	wound tissue repair	1641:1659	These results are demonstrated to provide a potential and possible pathway to promote wound tissue repair and regeneration.					
30755095	12	15	theme	cell	1479:1482	arg1	analysis					1484:1491	whole blood cell analysis	1467:1491	whole blood cell analysis	1467:1491	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	5	16	theme	plasma	653:658	arg1	spectrometry					665:676	inductively coupled plasma mass spectrometry	633:676	inductively coupled plasma mass spectrometry	633:676	The silver content of the dressing was measured by inductively coupled plasma mass spectrometry.					
30755095	12	17	theme	wound	1452:1456	arg1	exudate					1458:1464	wound exudate	1452:1464	wound exudate	1452:1464	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	11	18	from	effciency	1298:1306	arg1	wound					1346:1350	infected wound	1337:1350	infected wound	1337:1350	In addition, the healing effciency of the composite dressing on infected wound were examined in mice infected wound models.					
30755095	12	19	theme	blood	1473:1477	arg1	analysis					1484:1491	whole blood cell analysis	1467:1491	whole blood cell analysis	1467:1491	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	9	20	theme	total	1116:1120	arg1	property					1143:1150	The total protein slow-release property	1112:1150	The total protein slow-release property	1112:1150	The total protein slow-release property was measured using the BCA assay.					
30755095	12	21	theme	whole	1467:1471	arg1	analysis					1484:1491	whole blood cell analysis	1467:1491	whole blood cell analysis	1467:1491	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	1	22	theme	fibroin	144:150	arg1	scaffold					228:235	the scaffold	224:235	the scaffold	224:235	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	1	22	theme	fibroin	144:150	arg1	emulsion					152:159	a solution chitosan fibroin emulsion	124:159	a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs)	124:199	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	0	23	theme	wound	95:99	arg1	repair					101:106	infectious wound repair	84:106	infectious wound repair	84:106	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	5	24	theme	dressing	608:615	arg1	content					593:599	The silver content	582:599	The silver content of the dressing	582:615	The silver content of the dressing was measured by inductively coupled plasma mass spectrometry.					
30755095	0	25	theme	In	0:1	arg1	evaluation					21:30	In vitro and in vivo evaluation	0:30	In vitro and in vivo evaluation of new PRP antibacterial moisturizing	0:68	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	7	26	theme	tensile	868:874	arg1	strength					876:883	appropriate tensile strength	856:883	appropriate tensile strength	856:883	Relatively high porosity, favourable moisture retention capability and appropriate tensile strength were observed by measuring the physical and mechanical properties.					
30755095	10	27	theme	lower	1212:1216	arg1	sensitization					1218:1230	lower sensitization	1212:1230	lower sensitization	1212:1230	Good biocompatibility and lower sensitization were examined both in vitro and in vivo.					
30755095	12	28	theme	bacterial	1430:1438	arg1	cultures					1440:1447	bacterial cultures	1430:1447	bacterial cultures of wound exudate	1430:1464	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	6	29	theme	asymmetric	683:692	arg1	wettability					694:704	The asymmetric wettability	679:704	The asymmetric wettability of the composite dressing	679:730	The asymmetric wettability of the composite dressing was demonstrated by water contact angle measurement.					
30755095	11	30	theme	composite	1315:1323	arg1	dressing					1325:1332	the composite dressing	1311:1332	the composite dressing	1311:1332	In addition, the healing effciency of the composite dressing on infected wound were examined in mice infected wound models.					
30755095	2	31	theme	engineering	397:407	arg1	dressings					409:417	the tissue engineering dressings	386:417	the tissue engineering dressings	386:417	PRP was loaded onto the composite scaffold using a secondary lyophilization technology to prepare the tissue engineering dressings.					
30755095	7	32	theme	favourable	811:820	arg1	capability					841:850	favourable moisture retention capability	811:850	favourable moisture retention capability	811:850	Relatively high porosity, favourable moisture retention capability and appropriate tensile strength were observed by measuring the physical and mechanical properties.					
30755095	8	33	theme	effect	1086:1091	arg1	analysis					1093:1100	the antibacterial effect analysis	1068:1100	the antibacterial effect analysis	1068:1100	Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro.					
30755095	11	34	theme	infected	1337:1344	arg1	wound					1346:1350	infected wound	1337:1350	infected wound	1337:1350	In addition, the healing effciency of the composite dressing on infected wound were examined in mice infected wound models.					
30755095	1	35	theme	added	166:170	arg1	Nanoparticles					179:191	added Silver Nanoparticles	166:191	added Silver Nanoparticles (AgNPs)	166:199	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	1	35	theme	added	166:170	arg1	AgNPs					194:198	AgNPs	194:198	AgNPs	194:198	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	2	36	theme	tissue	390:395	arg1	dressings					409:417	the tissue engineering dressings	386:417	the tissue engineering dressings	386:417	PRP was loaded onto the composite scaffold using a secondary lyophilization technology to prepare the tissue engineering dressings.					
30755095	7	37	theme	moisture	822:829	arg1	capability					841:850	favourable moisture retention capability	811:850	favourable moisture retention capability	811:850	Relatively high porosity, favourable moisture retention capability and appropriate tensile strength were observed by measuring the physical and mechanical properties.					
30755095	11	38	theme	healing	1290:1296	arg1	effciency					1298:1306	the healing effciency	1286:1306	the healing effciency of the composite dressing on infected wound	1286:1350	In addition, the healing effciency of the composite dressing on infected wound were examined in mice infected wound models.					
30755095	8	39	theme	antibacterial	1072:1084	arg1	effect					1086:1091	the antibacterial effect	1068:1091	the antibacterial effect analysis	1068:1100	Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro.					
30755095	7	40	theme	appropriate	856:866	arg1	strength					876:883	appropriate tensile strength	856:883	appropriate tensile strength	856:883	Relatively high porosity, favourable moisture retention capability and appropriate tensile strength were observed by measuring the physical and mechanical properties.					
30755095	1	41	theme	Silver	172:177	arg1	Nanoparticles					179:191	added Silver Nanoparticles	166:191	added Silver Nanoparticles (AgNPs)	166:199	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	1	41	theme	Silver	172:177	arg1	AgNPs					194:198	AgNPs	194:198	AgNPs	194:198	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	13	42	theme	tissue	1647:1652	arg1	repair					1654:1659	wound tissue repair	1641:1659	wound tissue repair	1641:1659	These results are demonstrated to provide a potential and possible pathway to promote wound tissue repair and regeneration.					
30755095	1	43	theme	asymmetric	245:254	arg1	coating					256:262	an asymmetric coating	242:262	an asymmetric coating	242:262	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	7	44	theme	retention	831:839	arg1	capability					841:850	favourable moisture retention capability	811:850	favourable moisture retention capability	811:850	Relatively high porosity, favourable moisture retention capability and appropriate tensile strength were observed by measuring the physical and mechanical properties.					
30755095	10	45	theme	Good	1186:1189	arg1	biocompatibility					1191:1206	Good biocompatibility	1186:1206	Good biocompatibility	1186:1206	Good biocompatibility and lower sensitization were examined both in vitro and in vivo.					
30755095	12	46	theme	wound	1409:1413	arg1	healing					1415:1421	wound healing	1409:1421	wound healing rates	1409:1427	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	13	47	theme	possible	1613:1620	arg1	pathway					1622:1628	a potential and possible pathway	1597:1628	a potential and possible pathway to promote wound tissue repair and regeneration	1597:1676	These results are demonstrated to provide a potential and possible pathway to promote wound tissue repair and regeneration.					
30755095	2	48	theme	secondary	339:347	arg1	technology					364:373	a secondary lyophilization technology	337:373	a secondary lyophilization technology to prepare the tissue engineering dressings	337:417	PRP was loaded onto the composite scaffold using a secondary lyophilization technology to prepare the tissue engineering dressings.					
30755095	0	49	theme	in	13:14	arg1	evaluation					21:30	In vitro and in vivo evaluation	0:30	In vitro and in vivo evaluation of new PRP antibacterial moisturizing	0:68	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	6	50	theme	angle	766:770	arg1	measurement					772:782	water contact angle measurement	752:782	water contact angle measurement	752:782	The asymmetric wettability of the composite dressing was demonstrated by water contact angle measurement.					
30755095	2	51	theme	lyophilization	349:362	arg1	technology					364:373	a secondary lyophilization technology	337:373	a secondary lyophilization technology to prepare the tissue engineering dressings	337:417	PRP was loaded onto the composite scaffold using a secondary lyophilization technology to prepare the tissue engineering dressings.					
30755095	0	52	theme	new	35:37	arg1	moisturizing					57:68	new PRP antibacterial moisturizing	35:68	new PRP antibacterial moisturizing	35:68	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	7	53	theme	high	796:799	arg1	porosity					801:808	Relatively high porosity	785:808	Relatively high porosity	785:808	Relatively high porosity, favourable moisture retention capability and appropriate tensile strength were observed by measuring the physical and mechanical properties.					
30755095	5	54	theme	mass	660:663	arg1	spectrometry					665:676	inductively coupled plasma mass spectrometry	633:676	inductively coupled plasma mass spectrometry	633:676	The silver content of the dressing was measured by inductively coupled plasma mass spectrometry.					
30755095	12	55	theme	rates	1423:1427	arg1	cultures					1440:1447	bacterial cultures	1430:1447	bacterial cultures of wound exudate	1430:1464	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	12	55	theme	rates	1423:1427	arg1	examination					1510:1520	histological examination	1497:1520	histological examination	1497:1520	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	12	55	theme	rates	1423:1427	arg1	Analysis					1397:1404	Analysis	1397:1404	Analysis of wound healing rates	1397:1427	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	12	55	theme	rates	1423:1427	arg1	analysis					1484:1491	whole blood cell analysis	1467:1491	whole blood cell analysis	1467:1491	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	6	56	theme	contact	758:764	arg1	measurement					772:782	water contact angle measurement	752:782	water contact angle measurement	752:782	The asymmetric wettability of the composite dressing was demonstrated by water contact angle measurement.					
30755095	3	57	theme	transmission	453:464	arg1	microscope					475:484	a transmission electron microscope	451:484	a transmission electron microscope	451:484	AgNPs were characterized using a transmission electron microscope.					
30755095	11	58	dep	infected	1374:1381	arg1	wound					1383:1387	wound	1383:1387	wound	1383:1387	In addition, the healing effciency of the composite dressing on infected wound were examined in mice infected wound models.					
30755095	11	58	dep	infected	1374:1381	arg1	mice					1369:1372	mice	1369:1372	mice infected wound models	1369:1394	In addition, the healing effciency of the composite dressing on infected wound were examined in mice infected wound models.					
30755095	11	58	dep	infected	1374:1381	arg1	models					1389:1394	models	1389:1394	models	1389:1394	In addition, the healing effciency of the composite dressing on infected wound were examined in mice infected wound models.					
30755095	4	59	theme	dressing	521:528	arg1	morphologies					491:502	The morphologies	487:502	The morphologies of the composite dressing	487:528	The morphologies of the composite dressing were examined under a scanning electron microscope.					
30755095	11	60	theme	dressing	1325:1332	arg1	effciency					1298:1306	the healing effciency	1286:1306	the healing effciency of the composite dressing on infected wound	1286:1350	In addition, the healing effciency of the composite dressing on infected wound were examined in mice infected wound models.					
30755095	12	61	theme	healing	1415:1421	arg1	rates					1423:1427	wound healing rates	1409:1427	wound healing rates	1409:1427	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	6	62	theme	water	752:756	arg1	measurement					772:782	water contact angle measurement	752:782	water contact angle measurement	752:782	The asymmetric wettability of the composite dressing was demonstrated by water contact angle measurement.					
30755095	3	63	theme	electron	466:473	arg1	microscope					475:484	a transmission electron microscope	451:484	a transmission electron microscope	451:484	AgNPs were characterized using a transmission electron microscope.					
30755095	8	64	theme	Satisfactory	952:963	arg1	properties					979:988	Satisfactory antibacterial properties	952:988	Satisfactory antibacterial properties against various bacteria and microbial isolation performance	952:1049	Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro.					
30755095	4	65	theme	composite	511:519	arg1	dressing					521:528	the composite dressing	507:528	the composite dressing	507:528	The morphologies of the composite dressing were examined under a scanning electron microscope.					
30755095	12	66	theme	satisfactory	1533:1544	arg1	results					1546:1552	satisfactory results	1533:1552	satisfactory results	1533:1552	Analysis of wound healing rates, bacterial cultures of wound exudate, whole blood cell analysis and histological examination all showed satisfactory results.					
30755095	0	67	dep	in	13:14	arg1	vivo					16:19	vivo	16:19	vivo	16:19	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	8	68	theme	isolation	1029:1037	arg1	performance					1039:1049	microbial isolation performance	1019:1049	microbial isolation performance	1019:1049	Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro.					
30755095	8	69	theme	microbial	1019:1027	arg1	performance					1039:1049	microbial isolation performance	1019:1049	microbial isolation performance	1019:1049	Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro.					
30755095	1	70	with	emulsion	152:159	arg1	Nanoparticles					179:191	added Silver Nanoparticles	166:191	added Silver Nanoparticles (AgNPs)	166:199	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	1	70	with	emulsion	152:159	arg1	AgNPs					194:198	AgNPs	194:198	AgNPs	194:198	In this study, a solution chitosan fibroin emulsion with added Silver Nanoparticles (AgNPs) was freeze-dried to be the scaffold, and an asymmetric coating was formed on one side.					
30755095	8	71	theme	antibacterial	965:977	arg1	properties					979:988	Satisfactory antibacterial properties	952:988	Satisfactory antibacterial properties against various bacteria and microbial isolation performance	952:1049	Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro.					
30755095	9	72	theme	BCA	1175:1177	arg1	assay					1179:1183	the BCA assay	1171:1183	the BCA assay	1171:1183	The total protein slow-release property was measured using the BCA assay.					
30755095	0	73	theme	antibacterial	43:55	arg1	moisturizing					57:68	new PRP antibacterial moisturizing	35:68	new PRP antibacterial moisturizing	35:68	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	13	74	theme	potential	1599:1607	arg1	pathway					1622:1628	a potential and possible pathway	1597:1628	a potential and possible pathway to promote wound tissue repair and regeneration	1597:1676	These results are demonstrated to provide a potential and possible pathway to promote wound tissue repair and regeneration.					
30755095	8	75	theme	various	998:1004	arg1	bacteria					1006:1013	various bacteria	998:1013	various bacteria	998:1013	Satisfactory antibacterial properties against various bacteria and microbial isolation performance were observed by the antibacterial effect analysis in vitro.					
30755095	2	76	theme	composite	312:320	arg1	scaffold					322:329	the composite scaffold	308:329	the composite scaffold using a secondary lyophilization technology to prepare the tissue engineering dressings	308:417	PRP was loaded onto the composite scaffold using a secondary lyophilization technology to prepare the tissue engineering dressings.					
30755095	7	77	theme	mechanical	929:938	arg1	properties					940:949	the physical and mechanical properties	912:949	the physical and mechanical properties	912:949	Relatively high porosity, favourable moisture retention capability and appropriate tensile strength were observed by measuring the physical and mechanical properties.					
30755095	0	78	theme	PRP	39:41	arg1	moisturizing					57:68	new PRP antibacterial moisturizing	35:68	new PRP antibacterial moisturizing	35:68	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	0	79	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro and in vivo evaluation of new PRP antibacterial moisturizing dressings for infectious wound repair.					
30755095	6	80	theme	dressing	723:730	arg1	wettability					694:704	The asymmetric wettability	679:704	The asymmetric wettability of the composite dressing	679:730	The asymmetric wettability of the composite dressing was demonstrated by water contact angle measurement.					
29408004	8	0	theme	activity	1508:1515	arg1	%					1491:1491	74.7 and 62.5%	1478:1491	%	1491:1491	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	8	0	theme	activity	1508:1515	arg1	11cycles					1534:1541	11cycles	1534:1541	11cycles	1534:1541	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	8	0	theme	activity	1508:1515	arg1	activity					1508:1515	its initial activity	1496:1515	its initial activity	1496:1515	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	3	1	theme	neutral	527:533	arg1	pH					535:536	acidic and neutral pH	516:536	pH	535:536	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	0	2	theme	phospho-silicate	86:101	arg1	support					130:136	a novel inorganic support	112:136	a novel inorganic support	112:136	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	0	2	theme	phospho-silicate	86:101	arg1	glass					103:107	bioactive phospho-silicate glass	76:107	bioactive phospho-silicate glass	76:107	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	5	3	theme	Differential	1044:1055	arg1	DSC					1088:1090	DSC	1088:1090	DSC	1088:1090	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	3	theme	Differential	1044:1055	arg1	analysis					1078:1085	Differential scanning calorimetry analysis	1044:1085	Differential scanning calorimetry analysis (DSC)	1044:1091	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	4	theme	temperature	1014:1024	arg1	range					1026:1030	the temperature range	1010:1030	the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme	1010:1148	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	1	5	theme	bioactive	175:183	arg1	PS-glass					209:216	PS-glass	209:216	PS-glass	209:216	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	1	5	theme	bioactive	175:183	arg1	glass					202:206	bioactive phospho-silicate glass	175:206	bioactive phospho-silicate glass (PS-glass)	175:217	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	7	6	theme	Michaelis	1299:1307	arg1	Km					1319:1320	Km	1319:1320	Km	1319:1320	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	7	6	theme	Michaelis	1299:1307	arg1	constant					1309:1316	lower Michaelis constant	1293:1316	lower Michaelis constant (Km)	1293:1321	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	5	7	theme	higher	962:967	arg1	D-values					993:1000	D-values	993:1000	D-values	993:1000	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	7	theme	higher	962:967	arg1	time					987:990	higher decimal reduction time	962:990	higher decimal reduction time (D-values)	962:1001	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	8	8	dep	stability	1403:1411	arg1	The					1391:1393	The	1391:1393	The	1391:1393	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	7	9	theme	maximum	1324:1330	arg1	velocity					1332:1339	maximum velocity	1324:1339	maximum velocity (Vmax)	1324:1346	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	7	9	theme	maximum	1324:1330	arg1	Vmax					1342:1345	Vmax	1342:1345	Vmax	1342:1345	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	2	10	dep	spectroscopy	385:396	arg1	studies					445:451	studies	445:451	studies	445:451	Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies confirmed the glass-enzyme linkage.					
29408004	4	11	theme	immobilization	692:705	arg1	variables					707:715	Optimized immobilization variables	682:715	Optimized immobilization variables	682:715	Optimized immobilization variables enhanced the immobilization yield (IY) from 27.9 to 79.9% (2.9-fold).					
29408004	1	12	theme	covalent	275:282	arg1	binding					284:290	covalent binding	275:290	covalent binding	275:290	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	7	13	theme	catalytic	1352:1360	arg1	values					1383:1388	catalytic efficiency (Vmax/Km) values	1352:1388	catalytic efficiency (Vmax/Km) values	1352:1388	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	5	14	theme	calorimetry	1066:1076	arg1	DSC					1088:1090	DSC	1088:1090	DSC	1088:1090	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	14	theme	calorimetry	1066:1076	arg1	analysis					1078:1085	Differential scanning calorimetry analysis	1044:1085	Differential scanning calorimetry analysis (DSC)	1044:1091	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	6	15	theme	immobilized	1155:1165	arg1	stable					1178:1183	stable	1178:1183	stable	1178:1183	The immobilized enzyme was stable at a wide pH range (5.0-8.0).					
29408004	6	15	theme	immobilized	1155:1165	arg1	enzyme					1167:1172	The immobilized enzyme	1151:1172	The immobilized enzyme	1151:1172	The immobilized enzyme was stable at a wide pH range (5.0-8.0).					
29408004	5	16	theme	lower	890:894	arg1	Ea					915:916	Ea	915:916	Ea	915:916	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	16	theme	lower	890:894	arg1	energy					907:912	lower activation energy	890:912	lower activation energy (Ea)	890:917	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	17	theme	half-life	867:875	arg1	time					877:880	higher half-life time	860:880	higher half-life time (t1/2)	860:887	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	17	theme	half-life	867:875	arg1	t1/2					883:886	t1/2	883:886	t1/2	883:886	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	0	18	theme	inorganic	120:128	arg1	support					130:136	a novel inorganic support	112:136	a novel inorganic support	112:136	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	0	18	theme	inorganic	120:128	arg1	glass					103:107	bioactive phospho-silicate glass	76:107	bioactive phospho-silicate glass	76:107	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	7	19	theme	starch	1234:1239	arg1	hydrolysis					1241:1250	starch hydrolysis	1234:1250	starch hydrolysis	1234:1250	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	7	20	theme	efficiency	1362:1371	arg1	values					1383:1388	catalytic efficiency (Vmax/Km) values	1352:1388	catalytic efficiency (Vmax/Km) values	1352:1388	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	1	21	theme	phospho-silicate	185:200	arg1	PS-glass					209:216	PS-glass	209:216	PS-glass	209:216	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	1	21	theme	phospho-silicate	185:200	arg1	glass					202:206	bioactive phospho-silicate glass	175:206	bioactive phospho-silicate glass (PS-glass)	175:217	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	0	22	theme	novel	114:118	arg1	support					130:136	a novel inorganic support	112:136	a novel inorganic support	112:136	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	0	22	theme	novel	114:118	arg1	glass					103:107	bioactive phospho-silicate glass	76:107	bioactive phospho-silicate glass	76:107	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	5	23	theme	activation	896:905	arg1	Ea					915:916	Ea	915:916	Ea	915:916	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	23	theme	activation	896:905	arg1	energy					907:912	lower activation energy	890:912	lower activation energy (Ea)	890:917	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	7	24	theme	Vmax/Km	1374:1380	arg1	values					1383:1388	catalytic efficiency (Vmax/Km) values	1352:1388	catalytic efficiency (Vmax/Km) values	1352:1388	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	2	25	theme	scanning	410:417	arg1	SEM					440:442	SEM	440:442	SEM	440:442	Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies confirmed the glass-enzyme linkage.					
29408004	2	25	theme	scanning	410:417	arg1	microscopy					428:437	scanning electron microscopy	410:437	scanning electron microscopy (SEM)	410:443	Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies confirmed the glass-enzyme linkage.					
29408004	2	26	theme	glass-enzyme	467:478	arg1	linkage					480:486	the glass-enzyme linkage	463:486	the glass-enzyme linkage	463:486	Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies confirmed the glass-enzyme linkage.					
29408004	7	27	theme	Kinetic	1215:1221	arg1	studies					1223:1229	Kinetic studies	1215:1229	Kinetic studies of starch hydrolysis	1215:1250	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	3	28	theme	immobilization	579:592	arg1	variables					594:602	Some immobilization variables	574:602	Some immobilization variables	574:602	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	5	29	dep	40-60°C.	1035:1042	arg1	confirmed					1093:1101	confirmed	1093:1101	confirmed the thermalstability of the immobilized enzyme	1093:1148	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	0	30	theme	Enhancement	0:10	arg1	stability					12:20	Enhancement stability	0:20	Enhancement stability	0:20	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	8	31	theme	62.5	1487:1490	arg1	%					1491:1491	74.7 and 62.5%	1478:1491	%	1491:1491	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	8	31	theme	62.5	1487:1490	arg1	activity					1508:1515	its initial activity	1496:1515	its initial activity	1496:1515	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	9	32	theme	industrial	1630:1639	arg1	application					1641:1651	industrial application	1630:1651	industrial application	1630:1651	Enhanced α-amylase stabilities upon immobilization make it suitable for industrial application.					
29408004	5	33	theme	lower	920:924	arg1	kd					954:955	kd	954:955	kd	954:955	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	33	theme	lower	920:924	arg1	rate					948:951	lower deactivation constant rate	920:951	lower deactivation constant rate (kd)	920:956	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	34	theme	40-60°C.	1035:1042	arg1	range					1026:1030	the temperature range	1010:1030	the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme	1010:1148	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	0	35	theme	catalytic	26:34	arg1	activity					36:43	catalytic activity	26:43	catalytic activity	26:43	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	5	36	theme	decimal	969:975	arg1	D-values					993:1000	D-values	993:1000	D-values	993:1000	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	36	theme	decimal	969:975	arg1	time					987:990	higher decimal reduction time	962:990	higher decimal reduction time (D-values)	962:1001	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	8	37	theme	storage	1395:1401	arg1	stability					1403:1411	storage stability	1395:1411	storage stability	1395:1411	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	2	38	dep	transform	366:374	arg1	infrared					376:383	infrared	376:383	transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies	366:451	Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies confirmed the glass-enzyme linkage.					
29408004	5	39	theme	constant	939:946	arg1	kd					954:955	kd	954:955	kd	954:955	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	39	theme	constant	939:946	arg1	rate					948:951	lower deactivation constant rate	920:951	lower deactivation constant rate (kd)	920:956	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	40	theme	optimum	839:845	arg1	temperature					847:857	higher optimum temperature	832:857	higher optimum temperature	832:857	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	41	theme	higher	832:837	arg1	temperature					847:857	higher optimum temperature	832:857	higher optimum temperature	832:857	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	2	42	dep	Fourier	358:364	arg1	transform					366:374	transform	366:374	transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies	366:451	Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies confirmed the glass-enzyme linkage.					
29408004	7	43	theme	hydrolysis	1241:1250	arg1	studies					1223:1229	Kinetic studies	1215:1229	Kinetic studies of starch hydrolysis	1215:1250	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	5	44	contain	had	828:830	arg2	time					877:880	higher half-life time	860:880	higher half-life time (t1/2)	860:887	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	44	contain	had	828:830	arg2	D-values					993:1000	D-values	993:1000	D-values	993:1000	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	44	contain	had	828:830	arg2	t1/2					883:886	t1/2	883:886	t1/2	883:886	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	44	contain	had	828:830	arg2	energy					907:912	lower activation energy	890:912	lower activation energy (Ea)	890:917	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	44	contain	had	828:830	arg1	enzyme					821:826	the immobilized enzyme	805:826	the immobilized enzyme	805:826	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	44	contain	had	828:830	arg2	Ea					915:916	Ea	915:916	Ea	915:916	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	44	contain	had	828:830	arg2	rate					948:951	lower deactivation constant rate	920:951	lower deactivation constant rate (kd)	920:956	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	44	contain	had	828:830	arg2	temperature					847:857	higher optimum temperature	832:857	higher optimum temperature	832:857	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	44	contain	had	828:830	arg2	kd					954:955	kd	954:955	kd	954:955	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	44	contain	had	828:830	arg2	time					987:990	higher decimal reduction time	962:990	higher decimal reduction time (D-values)	962:1001	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	6	45	from	range	1198:1202	arg1	stable					1178:1183	stable	1178:1183	stable	1178:1183	The immobilized enzyme was stable at a wide pH range (5.0-8.0).					
29408004	6	45	from	range	1198:1202	arg1	enzyme					1167:1172	The immobilized enzyme	1151:1172	The immobilized enzyme	1151:1172	The immobilized enzyme was stable at a wide pH range (5.0-8.0).					
29408004	2	46	theme	electron	419:426	arg1	SEM					440:442	SEM	440:442	SEM	440:442	Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies confirmed the glass-enzyme linkage.					
29408004	2	46	theme	electron	419:426	arg1	microscopy					428:437	scanning electron microscopy	410:437	scanning electron microscopy (SEM)	410:443	Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) studies confirmed the glass-enzyme linkage.					
29408004	1	47	theme	poly	325:328	arg1	glutaraldehyde					306:319	glutaraldehyde	306:319	glutaraldehyde	306:319	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	1	47	theme	poly	325:328	arg1	glutaraldehyde					330:343	poly glutaraldehyde	325:343	poly glutaraldehyde	325:343	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	1	47	theme	poly	325:328	arg1	spacer					350:355	a spacer	348:355	a spacer	348:355	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	3	48	theme	Composite	663:671	arg1	alkaline					561:568	alkaline pH.	561:572	alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design)	561:679	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	3	48	theme	Composite	663:671	arg1	Design					673:678	Central Composite Design	655:678	Central Composite Design	655:678	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	3	49	theme	statistical	625:635	arg1	design					647:652	statistical factorial design	625:652	statistical factorial design	625:652	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	9	50	theme	Enhanced	1558:1565	arg1	stabilities					1577:1587	Enhanced α-amylase stabilities	1558:1587	Enhanced α-amylase stabilities upon immobilization	1558:1607	Enhanced α-amylase stabilities upon immobilization make it suitable for industrial application.					
29408004	1	51	theme	novel	224:228	arg1	enzyme					149:154	α-Amylase enzyme	139:154	α-Amylase enzyme	139:154	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	1	51	theme	novel	224:228	arg1	support					240:246	a novel inorganic support	222:246	a novel inorganic support	222:246	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	0	52	theme	α-amylase	60:68	arg1	stability					12:20	Enhancement stability	0:20	Enhancement stability	0:20	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	0	52	theme	α-amylase	60:68	arg1	activity					36:43	catalytic activity	26:43	catalytic activity	26:43	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	8	53	theme	initial	1500:1506	arg1	activity					1508:1515	its initial activity	1496:1515	its initial activity	1496:1515	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	5	54	theme	scanning	1057:1064	arg1	DSC					1088:1090	DSC	1088:1090	DSC	1088:1090	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	54	theme	scanning	1057:1064	arg1	analysis					1078:1085	Differential scanning calorimetry analysis	1044:1085	Differential scanning calorimetry analysis (DSC)	1044:1091	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	6	55	theme	pH	1195:1196	arg1	5.0-8.0					1205:1211	5.0-8.0	1205:1211	5.0-8.0	1205:1211	The immobilized enzyme was stable at a wide pH range (5.0-8.0).					
29408004	6	55	theme	pH	1195:1196	arg1	range					1198:1202	a wide pH range	1188:1202	a wide pH range (5.0-8.0)	1188:1212	The immobilized enzyme was stable at a wide pH range (5.0-8.0).					
29408004	8	56	theme	74.7	1478:1481	arg1	%					1491:1491	74.7 and 62.5%	1478:1491	%	1491:1491	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	8	56	theme	74.7	1478:1481	arg1	activity					1508:1515	its initial activity	1496:1515	its initial activity	1496:1515	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	3	57	theme	acidic	516:521	arg1	pH					535:536	acidic and neutral pH	516:536	pH	535:536	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	0	58	theme	immobilized	48:58	arg1	α-amylase					60:68	immobilized α-amylase	48:68	immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support	48:136	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	5	59	theme	immobilized	1131:1141	arg1	enzyme					1143:1148	the immobilized enzyme	1127:1148	the immobilized enzyme	1127:1148	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	7	60	theme	lower	1293:1297	arg1	Km					1319:1320	Km	1319:1320	Km	1319:1320	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	7	60	theme	lower	1293:1297	arg1	constant					1309:1316	lower Michaelis constant	1293:1316	lower Michaelis constant (Km)	1293:1321	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	6	61	theme	wide	1190:1193	arg1	5.0-8.0					1205:1211	5.0-8.0	1205:1211	5.0-8.0	1205:1211	The immobilized enzyme was stable at a wide pH range (5.0-8.0).					
29408004	6	61	theme	wide	1190:1193	arg1	range					1198:1202	a wide pH range	1188:1202	a wide pH range (5.0-8.0)	1188:1212	The immobilized enzyme was stable at a wide pH range (5.0-8.0).					
29408004	3	62	from	Dissolution	489:499	arg1	pH					535:536	acidic and neutral pH	516:536	pH	535:536	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	4	63	dep	79.9	769:772	arg1	to					766:767	to	766:767	to	766:767	Optimized immobilization variables enhanced the immobilization yield (IY) from 27.9 to 79.9% (2.9-fold).					
29408004	5	64	theme	enzyme	1143:1148	arg1	thermalstability					1107:1122	the thermalstability	1103:1122	the thermalstability of the immobilized enzyme	1103:1148	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	7	65	contain	had	1289:1291	arg2	values					1383:1388	catalytic efficiency (Vmax/Km) values	1352:1388	catalytic efficiency (Vmax/Km) values	1352:1388	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	7	65	contain	had	1289:1291	arg2	Km					1319:1320	Km	1319:1320	Km	1319:1320	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	7	65	contain	had	1289:1291	arg2	constant					1309:1316	lower Michaelis constant	1293:1316	lower Michaelis constant (Km)	1293:1321	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	7	65	contain	had	1289:1291	arg2	velocity					1332:1339	maximum velocity	1324:1339	maximum velocity (Vmax)	1324:1346	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	7	65	contain	had	1289:1291	arg1	enzyme					1282:1287	immobilized enzyme	1270:1287	immobilized enzyme	1270:1287	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	7	65	contain	had	1289:1291	arg2	Vmax					1342:1345	Vmax	1342:1345	Vmax	1342:1345	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	9	66	theme	α-amylase	1567:1575	arg1	stabilities					1577:1587	Enhanced α-amylase stabilities	1558:1587	Enhanced α-amylase stabilities upon immobilization	1558:1607	Enhanced α-amylase stabilities upon immobilization make it suitable for industrial application.					
29408004	1	67	theme	α-Amylase	139:147	arg1	enzyme					149:154	α-Amylase enzyme	139:154	α-Amylase enzyme	139:154	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	1	67	theme	α-Amylase	139:147	arg1	support					240:246	a novel inorganic support	222:246	a novel inorganic support	222:246	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	5	68	theme	deactivation	926:937	arg1	kd					954:955	kd	954:955	kd	954:955	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	68	theme	deactivation	926:937	arg1	rate					948:951	lower deactivation constant rate	920:951	lower deactivation constant rate (kd)	920:956	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	1	69	theme	inorganic	230:238	arg1	enzyme					149:154	α-Amylase enzyme	139:154	α-Amylase enzyme	139:154	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	1	69	theme	inorganic	230:238	arg1	support					240:246	a novel inorganic support	222:246	a novel inorganic support	222:246	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	1	70	dep	adsorption	260:269	arg1	methods					292:298	methods	292:298	methods	292:298	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
29408004	3	71	theme	Central	655:661	arg1	alkaline					561:568	alkaline pH.	561:572	alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design)	561:679	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	3	71	theme	Central	655:661	arg1	Design					673:678	Central Composite Design	655:678	Central Composite Design	655:678	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	8	72	theme	immobilized	1436:1446	arg1	enzyme					1448:1453	the immobilized enzyme	1432:1453	the immobilized enzyme	1432:1453	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	3	73	dep	alkaline	561:568	arg1	optimized					609:617	optimized	609:617	were optimized using statistical factorial design	604:652	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	3	73	dep	alkaline	561:568	arg1	pH.					570:572	alkaline pH.	561:572	alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design)	561:679	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	3	74	theme	PS-glass	504:511	arg1	Dissolution					489:499	Dissolution	489:499	Dissolution of PS-glass in acidic and neutral pH	489:536	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	0	75	theme	bioactive	76:84	arg1	support					130:136	a novel inorganic support	112:136	a novel inorganic support	112:136	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	0	75	theme	bioactive	76:84	arg1	glass					103:107	bioactive phospho-silicate glass	76:107	bioactive phospho-silicate glass	76:107	Enhancement stability and catalytic activity of immobilized α-amylase using bioactive phospho-silicate glass as a novel inorganic support.					
29408004	5	76	theme	higher	860:865	arg1	time					877:880	higher half-life time	860:880	higher half-life time (t1/2)	860:887	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	76	theme	higher	860:865	arg1	t1/2					883:886	t1/2	883:886	t1/2	883:886	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	3	77	theme	factorial	637:645	arg1	design					647:652	statistical factorial design	625:652	statistical factorial design	625:652	Dissolution of PS-glass in acidic and neutral pH is higher than that of alkaline pH. Some immobilization variables were optimized using statistical factorial design (Central Composite Design).					
29408004	7	78	theme	immobilized	1270:1280	arg1	enzyme					1282:1287	immobilized enzyme	1270:1287	immobilized enzyme	1270:1287	Kinetic studies of starch hydrolysis demonstrated that immobilized enzyme had lower Michaelis constant (Km), maximum velocity (Vmax) and catalytic efficiency (Vmax/Km) values.					
29408004	4	79	theme	immobilization	730:743	arg1	yield					745:749	the immobilization yield	726:749	the immobilization yield (IY)	726:754	Optimized immobilization variables enhanced the immobilization yield (IY) from 27.9 to 79.9% (2.9-fold).					
29408004	4	79	theme	immobilization	730:743	arg1	IY					752:753	IY	752:753	IY	752:753	Optimized immobilization variables enhanced the immobilization yield (IY) from 27.9 to 79.9% (2.9-fold).					
29408004	4	80	theme	Optimized	682:690	arg1	variables					707:715	Optimized immobilization variables	682:715	Optimized immobilization variables	682:715	Optimized immobilization variables enhanced the immobilization yield (IY) from 27.9 to 79.9% (2.9-fold).					
29408004	8	81	theme	enzyme	1448:1453	arg1	reusability					1417:1427	reusability	1417:1427	reusability	1417:1427	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	8	81	theme	enzyme	1448:1453	arg1	stability					1403:1411	storage stability	1395:1411	storage stability	1395:1411	The storage stability and reusability of the immobilized enzyme were found to be about 74.7 and 62.5% of its initial activity after 28days and 11cycles, respectively.					
29408004	5	82	theme	reduction	977:985	arg1	D-values					993:1000	D-values	993:1000	D-values	993:1000	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	82	theme	reduction	977:985	arg1	time					987:990	higher decimal reduction time	962:990	higher decimal reduction time (D-values)	962:1001	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	5	83	theme	immobilized	809:819	arg1	enzyme					821:826	the immobilized enzyme	805:826	the immobilized enzyme	805:826	It was found that the immobilized enzyme had higher optimum temperature, higher half-life time (t1/2), lower activation energy (Ea), lower deactivation constant rate (kd) and higher decimal reduction time (D-values) within the temperature range of 40-60°C. Differential scanning calorimetry analysis (DSC) confirmed the thermalstability of the immobilized enzyme.					
29408004	1	84	theme	physical	251:258	arg1	adsorption					260:269	physical adsorption	251:269	physical adsorption	251:269	α-Amylase enzyme was immobilized on bioactive phospho-silicate glass (PS-glass) as a novel inorganic support by physical adsorption and covalent binding methods using glutaraldehyde and poly glutaraldehyde as a spacer.					
31107901	4	0	theme	hindgut	878:884	arg1	tissues					886:892	hindgut tissues	878:892	hindgut tissues from Triatoma infestans	878:916	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	1	theme	attachment	1075:1084	arg1	rates					1086:1090	improved attachment rates	1066:1090	improved attachment rates (~2-3 fold)	1066:1102	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	1	theme	attachment	1075:1084	arg1	fold					1098:1101	~2-3 fold	1093:1101	~2-3 fold	1093:1101	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	2	theme	ampoules	1388:1395	arg1	lining					1358:1363	the inner lining	1348:1363	the inner lining of T. infestans rectal ampoules in ex vivo binding assays	1348:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	3	3	theme	surface	498:504	arg1	glycoproteins					506:518	the major surface glycoproteins	488:518	the major surface glycoproteins from T. cruzi insect-dwelling forms	488:554	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	3	theme	surface	498:504	arg1	mucins					480:485	Gp35/50 kDa mucins	468:485	Gp35/50 kDa mucins	468:485	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	6	4	from	O-glycans	2065:2073	arg1	clone					2117:2121	the former but not the latter parasite clone	2078:2121	the former but not the latter parasite clone	2078:2121	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	6	5	theme	residues	2029:2036	arg1	presence					2012:2019	the presence	2008:2019	the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone	2008:2121	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	4	6	theme	inner	1352:1356	arg1	lining					1358:1363	the inner lining	1348:1363	the inner lining of T. infestans rectal ampoules in ex vivo binding assays	1348:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	1	7	theme	BACKGROUND	106:115	arg1	cruzi					129:133	BACKGROUND Trypanosoma cruzi	106:133	BACKGROUND Trypanosoma cruzi	106:133	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	1	7	theme	BACKGROUND	106:115	arg1	agent					140:144	the agent	136:144	the agent of Chagas disease	136:162	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	1	7	theme	BACKGROUND	106:115	arg1	parasite					180:187	a protozoan parasite	168:187	a protozoan parasite transmitted to humans by blood-sucking triatomine vectors	168:245	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	4	8	theme	appropriate	1135:1145	arg1	controls					1158:1165	appropriate transgenic controls	1135:1165	appropriate transgenic controls	1135:1165	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	3	9	theme	mammal-infective	703:718	arg1	forms					720:724	mammal-infective forms	703:724	mammal-infective forms	703:724	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	4	10	dep	exhibited	1056:1064	arg1	compounds					1238:1246	certain chemically synthesized compounds	1207:1246	certain chemically synthesized compounds	1207:1246	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	11	theme	Brener	1934:1939	arg1	epimastigotes					1941:1953	CL Brener epimastigotes	1931:1953	CL Brener epimastigotes	1931:1953	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	2	12	theme	epidemiological	295:309	arg1	relevance					311:319	its utmost biological and epidemiological relevance	269:319	its utmost biological and epidemiological relevance	269:319	However, and despite its utmost biological and epidemiological relevance, T. cruzi development inside the digestive tract of the insect remains a poorly understood process.					
31107901	4	13	from	assays	1416:1421	arg1	lining					1358:1363	the inner lining	1348:1363	the inner lining of T. infestans rectal ampoules in ex vivo binding assays	1348:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	2	14	theme	biological	280:289	arg1	relevance					311:319	its utmost biological and epidemiological relevance	269:319	its utmost biological and epidemiological relevance	269:319	However, and despite its utmost biological and epidemiological relevance, T. cruzi development inside the digestive tract of the insect remains a poorly understood process.					
31107901	4	15	theme	immunofluorescence	943:960	arg1	assays					962:967	indirect immunofluorescence assays	934:967	indirect immunofluorescence assays	934:967	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	16	theme	ex	1400:1401	arg1	assays					1416:1421	ex vivo binding assays	1400:1421	ex vivo binding assays	1400:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	17	attach	derived	1248:1254	arg2	epimastigotes					985:997	transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds	974:1246	transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins	974:1278	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	17	attach	derived	1248:1254	arg1	mucins					1273:1278	Gp35/50 kDa mucins	1261:1278	Gp35/50 kDa mucins	1261:1278	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	18	theme	binding	1408:1414	arg1	assays					1416:1421	ex vivo binding assays	1400:1421	ex vivo binding assays	1400:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	1	19	theme	Trypanosoma	117:127	arg1	cruzi					129:133	BACKGROUND Trypanosoma cruzi	106:133	BACKGROUND Trypanosoma cruzi	106:133	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	1	19	theme	Trypanosoma	117:127	arg1	agent					140:144	the agent	136:144	the agent of Chagas disease	136:162	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	1	19	theme	Trypanosoma	117:127	arg1	parasite					180:187	a protozoan parasite	168:187	a protozoan parasite transmitted to humans by blood-sucking triatomine vectors	168:245	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	4	20	theme	certain	1207:1213	arg1	compounds					1238:1246	certain chemically synthesized compounds	1207:1246	certain chemically synthesized compounds	1207:1246	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	21	theme	Experimental	727:738	arg1	evidence					740:747	Experimental evidence	727:747	Experimental evidence supporting this conclusion	727:774	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	21	theme	Experimental	727:738	arg1	follows					799:805	follows	799:805	follows	799:805	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	22	from	infestans	908:916	arg1	tissues					886:892	hindgut tissues	878:892	hindgut tissues from Triatoma infestans	878:916	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	3	23	theme	insect-dwelling	534:548	arg1	forms					550:554	T. cruzi insect-dwelling forms	525:554	T. cruzi insect-dwelling forms	525:554	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	8	24	theme	Gp35/50	2489:2495	arg1	mucins					2501:2506	Gp35/50 kDa mucins	2489:2506	Gp35/50 kDa mucins	2489:2506	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	4	25	theme	wild-type	1174:1182	arg1	counterparts					1184:1195	wild-type counterparts	1174:1195	wild-type counterparts	1174:1195	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	26	theme	receptor	1482:1489	arg1	s					1491:1491	insect receptor(s)	1475:1492	insect receptor(s)	1475:1492	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	7	27	gly	O-glycosylation	2296:2310	arg1	mucins					2327:2332	Gp35/50 kDa mucins	2315:2332	Gp35/50 kDa mucins	2315:2332	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	4	28	dep	summarized	785:794	arg1	ii					970:971	ii	970:971	ii	970:971	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	28	dep	summarized	785:794	arg1	i					808:808	i	808:808	i	808:808	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	28	dep	summarized	785:794	arg1	able					1285:1288	able	1285:1288	able	1285:1288	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	28	dep	summarized	785:794	arg1	interacted					862:871	interacted	862:871	interacted	862:871	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	29	theme	Gp35/50	1015:1021	arg1	mucins					1027:1032	over-expressing Gp35/50 kDa mucins	999:1032	over-expressing Gp35/50 kDa mucins on their surface coat	999:1054	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	30	from	ampoules	1388:1395	arg1	assays					1416:1421	ex vivo binding assays	1400:1421	ex vivo binding assays	1400:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	31	theme	hindgut	1969:1975	arg1	tissues					1977:1983	triatomine hindgut tissues	1958:1983	triatomine hindgut tissues	1958:1983	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	1	32	theme	Chagas	149:154	arg1	disease					156:162	Chagas disease	149:162	Chagas disease	149:162	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	3	33	theme	ampoule	642:648	arg1	cuticle					609:615	the internal cuticle	596:615	the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms	596:724	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	4	34	theme	Gp35/50	1261:1267	arg1	mucins					1273:1278	Gp35/50 kDa mucins	1261:1278	Gp35/50 kDa mucins	1261:1278	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	35	theme	kDa	2053:2055	arg1	mucins					2057:2062	Gp35/50 kDa mucins	2045:2062	the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone	2041:2121	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	3	36	from	forms	550:554	arg1	glycoproteins					506:518	the major surface glycoproteins	488:518	the major surface glycoproteins from T. cruzi insect-dwelling forms	488:554	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	36	from	forms	550:554	arg1	mucins					480:485	Gp35/50 kDa mucins	468:485	Gp35/50 kDa mucins	468:485	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	8	37	theme	biosynthetic	2524:2535	arg1	absent					2555:2560	absent	2555:2560	absent	2555:2560	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	8	37	theme	biosynthetic	2524:2535	arg1	pathway					2537:2543	the Galf biosynthetic pathway	2515:2543	the Galf biosynthetic pathway	2515:2543	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	2	38	theme	understood	401:410	arg1	process					412:418	a poorly understood process	392:418	a poorly understood process	392:418	However, and despite its utmost biological and epidemiological relevance, T. cruzi development inside the digestive tract of the insect remains a poorly understood process.					
31107901	7	39	theme	Gp35/50	2315:2321	arg1	kDa					2323:2325	kDa	2323:2325	kDa	2323:2325	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	5	40	theme	main	1704:1707	arg1	peptide					1513:1519	A solvent-exposed peptide	1495:1519	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans	1495:1683	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	40	theme	main	1704:1707	arg1	determinants					1718:1729	main adhesion determinants	1704:1729	main adhesion determinants for these molecules	1704:1749	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	6	41	theme	parasite	2108:2115	arg1	clone					2117:2121	the former but not the latter parasite clone	2078:2121	the former but not the latter parasite clone	2078:2121	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	0	42	dep	infestans	80:88	arg1	ampoule					97:103	rectal ampoule	90:103	the Triatoma infestans rectal ampoule	67:103	Trypanosoma cruzi surface mucins are involved in the attachment to the Triatoma infestans rectal ampoule.					
31107901	4	43	theme	synthesized	1226:1236	arg1	compounds					1238:1246	certain chemically synthesized compounds	1207:1246	certain chemically synthesized compounds	1207:1246	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	2	44	dep	T.	322:323	arg1	cruzi					325:329	cruzi	325:329	cruzi	325:329	However, and despite its utmost biological and epidemiological relevance, T. cruzi development inside the digestive tract of the insect remains a poorly understood process.					
31107901	8	45	theme	appealing	2589:2597	arg1	targets					2599:2605	appealing targets	2589:2605	appealing targets for the development of T. cruzi transmission-blocking strategies	2589:2670	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	0	46	theme	Trypanosoma	0:10	arg1	mucins					26:31	Trypanosoma cruzi surface mucins	0:31	Trypanosoma cruzi surface mucins	0:31	Trypanosoma cruzi surface mucins are involved in the attachment to the Triatoma infestans rectal ampoule.					
31107901	5	47	dep	Galfβ1-4[Galpβ1-6	1630:1646	arg1	GlcNAcα					1648:1654	GlcNAcα	1648:1654	GlcNAcα	1648:1654	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	4	48	theme	improved	1066:1073	arg1	rates					1086:1090	improved attachment rates	1066:1090	improved attachment rates (~2-3 fold)	1066:1102	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	48	theme	improved	1066:1073	arg1	fold					1098:1101	~2-3 fold	1093:1101	~2-3 fold	1093:1101	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	0	49	theme	surface	18:24	arg1	mucins					26:31	Trypanosoma cruzi surface mucins	0:31	Trypanosoma cruzi surface mucins	0:31	Trypanosoma cruzi surface mucins are involved in the attachment to the Triatoma infestans rectal ampoule.					
31107901	6	50	theme	GlcNAcα	1819:1825	arg1	derivative					1827:1836	a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative	1789:1836	a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative	1789:1836	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	5	51	theme	kDa	1554:1556	arg1	scaffolds					1573:1581	the Gp35/50 kDa mucins protein scaffolds	1542:1581	the Gp35/50 kDa mucins protein scaffolds	1542:1581	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	7	52	theme	inter-strain	2265:2276	arg1	variations					2278:2287	inter-strain variations	2265:2287	inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins	2265:2332	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	6	53	theme	Galfβ1-4[Galpβ1-6	1801:1817	arg1	derivative					1827:1836	a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative	1789:1836	a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative	1789:1836	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	5	54	theme	smugS	1522:1526	arg1	peptide					1513:1519	A solvent-exposed peptide	1495:1519	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans	1495:1683	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	54	theme	smugS	1522:1526	arg1	peptide					1528:1534	smugS peptide	1522:1534	smugS peptide	1522:1534	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	3	55	theme	rectal	635:640	arg1	ampoule					642:648	the triatomine rectal ampoule	620:648	the triatomine rectal ampoule	620:648	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	55	theme	rectal	635:640	arg1	step					662:665	a critical step	651:665	a critical step leading to its differentiation into mammal-infective forms	651:724	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	4	56	theme	Gp35/50	834:840	arg1	mucins					846:851	native and recombinant Gp35/50 kDa mucins	811:851	mucins	846:851	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	8	57	theme	T.	2630:2631	arg1	strategies					2661:2670	T. cruzi transmission-blocking strategies	2630:2670	T. cruzi transmission-blocking strategies	2630:2670	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	4	58	from	mucins	1027:1032	arg1	coat					1051:1054	their surface coat	1037:1054	their surface coat	1037:1054	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	5	59	from	glycans	1677:1683	arg1	determinants					1718:1729	main adhesion determinants	1704:1729	main adhesion determinants for these molecules	1704:1749	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	59	from	glycans	1677:1683	arg1	peptide					1513:1519	A solvent-exposed peptide	1495:1519	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans	1495:1683	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	59	from	glycans	1677:1683	arg1	peptide					1528:1534	smugS peptide	1522:1534	smugS peptide	1522:1534	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	8	60	theme	transmission-blocking	2639:2659	arg1	strategies					2661:2670	T. cruzi transmission-blocking strategies	2630:2670	T. cruzi transmission-blocking strategies	2630:2670	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	2	61	theme	T.	322:323	arg1	development					331:341	T. cruzi development	322:341	T. cruzi development inside the digestive tract of the insect	322:382	However, and despite its utmost biological and epidemiological relevance, T. cruzi development inside the digestive tract of the insect remains a poorly understood process.					
31107901	4	62	theme	such	1107:1110	arg1	tissues					1112:1118	such tissues	1107:1118	such tissues	1107:1118	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	5	63	theme	branched	1589:1596	arg1	Galfβ1-4[Galpβ1-6					1630:1646	Galfβ1-4[Galpβ1-6	1630:1646	Galfβ1-4[Galpβ1-6	1630:1646	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	63	theme	branched	1589:1596	arg1	trisaccharide					1615:1627	a branched, Galf-containing trisaccharide	1587:1627	a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα)	1587:1655	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	3	64	theme	critical	653:660	arg1	ampoule					642:648	the triatomine rectal ampoule	620:648	the triatomine rectal ampoule	620:648	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	64	theme	critical	653:660	arg1	step					662:665	a critical step	651:665	a critical step leading to its differentiation into mammal-infective forms	651:724	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	7	65	theme	cruzi-triatomine	2219:2234	arg1	interplay					2236:2244	T. cruzi-triatomine interplay	2216:2244	T. cruzi-triatomine interplay	2216:2244	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	7	66	theme	T.	2216:2217	arg1	interplay					2236:2244	T. cruzi-triatomine interplay	2216:2244	T. cruzi-triatomine interplay	2216:2244	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	1	67	theme	blood-sucking	214:226	arg1	vectors					239:245	blood-sucking triatomine vectors	214:245	blood-sucking triatomine vectors	214:245	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	4	68	theme	T.	1368:1369	arg1	infestans					1371:1379	T. infestans	1368:1379	T. infestans rectal ampoules in ex vivo binding assays	1368:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	3	69	theme	major	492:496	arg1	glycoproteins					506:518	the major surface glycoproteins	488:518	the major surface glycoproteins from T. cruzi insect-dwelling forms	488:554	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	69	theme	major	492:496	arg1	mucins					480:485	Gp35/50 kDa mucins	468:485	Gp35/50 kDa mucins	468:485	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	4	70	dep	ex	1400:1401	arg1	vivo					1403:1406	vivo	1403:1406	vivo	1403:1406	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	8	71	from	absent	2555:2560	arg1	mammals					2565:2571	mammals	2565:2571	mammals	2565:2571	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	8	71	from	absent	2555:2560	arg1	insects					2577:2583	insects	2577:2583	insects	2577:2583	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	5	72	theme	protein	1565:1571	arg1	scaffolds					1573:1581	the Gp35/50 kDa mucins protein scaffolds	1542:1581	the Gp35/50 kDa mucins protein scaffolds	1542:1581	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	4	73	theme	rectal	1381:1386	arg1	ampoules					1388:1395	T. infestans rectal ampoules	1368:1395	T. infestans rectal ampoules in ex vivo binding assays	1368:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	74	theme	former	2082:2087	arg1	clone					2117:2121	the former but not the latter parasite clone	2078:2121	the former but not the latter parasite clone	2078:2121	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	2	75	theme	utmost	273:278	arg1	relevance					311:319	its utmost biological and epidemiological relevance	269:319	its utmost biological and epidemiological relevance	269:319	However, and despite its utmost biological and epidemiological relevance, T. cruzi development inside the digestive tract of the insect remains a poorly understood process.					
31107901	4	76	theme	transgenic	1147:1156	arg1	controls					1158:1165	appropriate transgenic controls	1135:1165	appropriate transgenic controls	1135:1165	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	77	theme	oligosaccharides	1844:1859	arg1	addition					1777:1784	exogenous addition	1767:1784	exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure	1767:1885	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	3	78	theme	T.	525:526	arg1	forms					550:554	T. cruzi insect-dwelling forms	525:554	T. cruzi insect-dwelling forms	525:554	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	1	79	theme	disease	156:162	arg1	cruzi					129:133	BACKGROUND Trypanosoma cruzi	106:133	BACKGROUND Trypanosoma cruzi	106:133	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	1	79	theme	disease	156:162	arg1	agent					140:144	the agent	136:144	the agent of Chagas disease	136:162	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	4	80	theme	kDa	842:844	arg1	mucins					846:851	native and recombinant Gp35/50 kDa mucins	811:851	mucins	846:851	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	81	theme	epimastigotes	1941:1953	arg1	attachment					1900:1909	the attachment	1896:1909	the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues	1896:1983	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	8	82	from	insects	2577:2583	arg1	absent					2555:2560	absent	2555:2560	absent	2555:2560	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	8	82	from	insects	2577:2583	arg1	pathway					2537:2543	the Galf biosynthetic pathway	2515:2543	the Galf biosynthetic pathway	2515:2543	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	7	83	theme	parasite	2361:2368	arg1	differentiation					2370:2384	parasite differentiation	2361:2384	parasite differentiation	2361:2384	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	6	84	contain	containing	1861:1870	arg1	oligosaccharides					1844:1859	oligosaccharides	1844:1859	oligosaccharides containing this structure	1844:1885	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	6	84	contain	containing	1861:1870	arg2	structure					1877:1885	this structure	1872:1885	this structure	1872:1885	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	6	85	theme	CL	1931:1932	arg1	epimastigotes					1941:1953	CL Brener epimastigotes	1931:1953	CL Brener epimastigotes	1931:1953	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	8	86	from	mammals	2565:2571	arg1	absent					2555:2560	absent	2555:2560	absent	2555:2560	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	8	86	from	mammals	2565:2571	arg1	pathway					2537:2543	the Galf biosynthetic pathway	2515:2543	the Galf biosynthetic pathway	2515:2543	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	1	87	theme	protozoan	170:178	arg1	cruzi					129:133	BACKGROUND Trypanosoma cruzi	106:133	BACKGROUND Trypanosoma cruzi	106:133	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	1	87	theme	protozoan	170:178	arg1	parasite					180:187	a protozoan parasite	168:187	a protozoan parasite transmitted to humans by blood-sucking triatomine vectors	168:245	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31107901	7	88	theme	mammalian	2433:2441	arg1	host					2443:2446	the mammalian host	2429:2446	the mammalian host	2429:2446	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	7	89	from	variations	2278:2287	arg1	O-glycosylation					2296:2310	the O-glycosylation	2292:2310	the O-glycosylation of Gp35/50 kDa mucins	2292:2332	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	4	90	theme	indirect	934:941	arg1	immunofluorescence					943:960	indirect immunofluorescence	934:960	indirect immunofluorescence assays	934:967	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	91	theme	Dm28c	1914:1918	arg1	attachment					1900:1909	the attachment	1896:1909	the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues	1896:1983	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	3	92	theme	parasite	573:580	arg1	attachment					582:591	parasite attachment	573:591	parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms	573:724	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	93	dep	T.	525:526	arg1	cruzi					528:532	cruzi	528:532	cruzi	528:532	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	4	94	theme	Triatoma	899:906	arg1	infestans					908:916	Triatoma infestans	899:916	Triatoma infestans	899:916	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	7	95	theme	parasite	2400:2407	arg1	transmissibility					2409:2424	parasite transmissibility	2400:2424	parasite transmissibility to the mammalian host	2400:2446	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	4	96	dep	epimastigotes	985:997	arg1	exhibited					1056:1064	exhibited	1056:1064	exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts	1056:1195	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	4	96	dep	epimastigotes	985:997	arg1	iii					1202:1204	iii	1202:1204	iii	1202:1204	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	8	97	theme	kDa	2497:2499	arg1	mucins					2501:2506	Gp35/50 kDa mucins	2489:2506	Gp35/50 kDa mucins	2489:2506	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	7	98	theme	mucins	2327:2332	arg1	O-glycosylation					2296:2310	the O-glycosylation	2292:2310	the O-glycosylation of Gp35/50 kDa mucins	2292:2332	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	3	99	theme	kDa	476:478	arg1	glycoproteins					506:518	the major surface glycoproteins	488:518	the major surface glycoproteins from T. cruzi insect-dwelling forms	488:554	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	99	theme	kDa	476:478	arg1	mucins					480:485	Gp35/50 kDa mucins	468:485	Gp35/50 kDa mucins	468:485	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	100	theme	internal	600:607	arg1	cuticle					609:615	the internal cuticle	596:615	the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms	596:724	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	4	101	theme	transgenic	974:983	arg1	epimastigotes					985:997	transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds	974:1246	transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins	974:1278	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	5	102	theme	O-linked	1668:1675	arg1	glycans					1677:1683	their O-linked glycans	1662:1683	their O-linked glycans	1662:1683	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	4	103	theme	insect	1475:1480	arg1	s					1491:1491	insect receptor(s)	1475:1492	insect receptor(s)	1475:1492	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	0	104	theme	Triatoma	71:78	arg1	infestans					80:88	the Triatoma infestans rectal ampoule	67:103	the Triatoma infestans rectal ampoule	67:103	Trypanosoma cruzi surface mucins are involved in the attachment to the Triatoma infestans rectal ampoule.					
31107901	8	105	theme	Galf	2519:2522	arg1	absent					2555:2560	absent	2555:2560	absent	2555:2560	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	8	105	theme	Galf	2519:2522	arg1	pathway					2537:2543	the Galf biosynthetic pathway	2515:2543	the Galf biosynthetic pathway	2515:2543	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	4	106	theme	over-expressing	999:1013	arg1	mucins					1027:1032	over-expressing Gp35/50 kDa mucins	999:1032	over-expressing Gp35/50 kDa mucins on their surface coat	999:1054	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	107	theme	exogenous	1767:1775	arg1	addition					1777:1784	exogenous addition	1767:1784	exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure	1767:1885	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	5	108	theme	solvent-exposed	1497:1511	arg1	determinants					1718:1729	main adhesion determinants	1704:1729	main adhesion determinants for these molecules	1704:1749	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	108	theme	solvent-exposed	1497:1511	arg1	peptide					1513:1519	A solvent-exposed peptide	1495:1519	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans	1495:1683	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	108	theme	solvent-exposed	1497:1511	arg1	peptide					1528:1534	smugS peptide	1522:1534	smugS peptide	1522:1534	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	4	109	theme	kDa	1269:1271	arg1	mucins					1273:1278	Gp35/50 kDa mucins	1261:1278	Gp35/50 kDa mucins	1261:1278	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	110	theme	triatomine	1958:1967	arg1	tissues					1977:1983	triatomine hindgut tissues	1958:1983	triatomine hindgut tissues	1958:1983	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	0	111	theme	rectal	90:95	arg1	ampoule					97:103	rectal ampoule	90:103	the Triatoma infestans rectal ampoule	67:103	Trypanosoma cruzi surface mucins are involved in the attachment to the Triatoma infestans rectal ampoule.					
31107901	6	112	theme	Gp35/50	2045:2051	arg1	mucins					2057:2062	Gp35/50 kDa mucins	2045:2062	the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone	2041:2121	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	3	113	gly	glycoproteins	506:518	arg1	glycoproteins					506:518	the major surface glycoproteins	488:518	the major surface glycoproteins from T. cruzi insect-dwelling forms	488:554	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	113	gly	glycoproteins	506:518	arg1	mucins					480:485	Gp35/50 kDa mucins	468:485	Gp35/50 kDa mucins	468:485	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	4	114	from	lining	1358:1363	arg1	assays					1416:1421	ex vivo binding assays	1400:1421	ex vivo binding assays	1400:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	7	115	theme	kDa	2323:2325	arg1	mucins					2327:2332	Gp35/50 kDa mucins	2315:2332	Gp35/50 kDa mucins	2315:2332	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	2	116	theme	insect	377:382	arg1	tract					364:368	the digestive tract	350:368	the digestive tract of the insect	350:382	However, and despite its utmost biological and epidemiological relevance, T. cruzi development inside the digestive tract of the insect remains a poorly understood process.					
31107901	6	117	theme	latter	2101:2106	arg1	clone					2117:2121	the former but not the latter parasite clone	2078:2121	the former but not the latter parasite clone	2078:2121	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	6	118	attach	attachment	1900:1909	arg1	tissues					1977:1983	triatomine hindgut tissues	1958:1983	triatomine hindgut tissues	1958:1983	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	6	118	attach	attachment	1900:1909	arg2	Dm28c					1914:1918	Dm28c	1914:1918	Dm28c	1914:1918	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	4	119	theme	surface	1043:1049	arg1	coat					1051:1054	their surface coat	1037:1054	their surface coat	1037:1054	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	6	120	theme	Galf	2024:2027	arg1	residues					2029:2036	Galf residues	2024:2036	Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone	2024:2121	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	3	121	theme	METHODS/PRINCIPLE	421:437	arg1	FINDINGS					439:446	METHODS/PRINCIPLE FINDINGS	421:446	METHODS/PRINCIPLE FINDINGS	421:446	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	7	122	from	differences	2346:2356	arg1	differentiation					2370:2384	parasite differentiation	2361:2384	parasite differentiation	2361:2384	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	7	122	from	differences	2346:2356	arg1	transmissibility					2409:2424	parasite transmissibility	2400:2424	parasite transmissibility to the mammalian host	2400:2446	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	6	123	theme	derivative	1827:1836	arg1	addition					1777:1784	exogenous addition	1767:1784	exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure	1767:1885	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	5	124	theme	Gp35/50	1546:1552	arg1	kDa					1554:1556	kDa	1554:1556	kDa	1554:1556	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	4	125	theme	epimastigote	1321:1332	arg1	attachment					1334:1343	epimastigote attachment	1321:1343	epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays	1321:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	3	126	theme	Gp35/50	468:474	arg1	glycoproteins					506:518	the major surface glycoproteins	488:518	the major surface glycoproteins from T. cruzi insect-dwelling forms	488:554	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	126	theme	Gp35/50	468:474	arg1	mucins					480:485	Gp35/50 kDa mucins	468:485	Gp35/50 kDa mucins	468:485	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	5	127	dep	branched	1589:1596	arg1	Galf-containing					1599:1613	Galf-containing	1599:1613	Galf-containing	1599:1613	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	4	128	theme	kDa	1023:1025	arg1	mucins					1027:1032	over-expressing Gp35/50 kDa mucins	999:1032	over-expressing Gp35/50 kDa mucins on their surface coat	999:1054	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	5	129	theme	mucins	1558:1563	arg1	scaffolds					1573:1581	the Gp35/50 kDa mucins protein scaffolds	1542:1581	the Gp35/50 kDa mucins protein scaffolds	1542:1581	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	3	130	theme	triatomine	624:633	arg1	ampoule					642:648	the triatomine rectal ampoule	620:648	the triatomine rectal ampoule	620:648	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	3	130	theme	triatomine	624:633	arg1	step					662:665	a critical step	651:665	a critical step leading to its differentiation into mammal-infective forms	651:724	METHODS/PRINCIPLE FINDINGS Here we showed that Gp35/50 kDa mucins, the major surface glycoproteins from T. cruzi insect-dwelling forms, are involved in parasite attachment to the internal cuticle of the triatomine rectal ampoule, a critical step leading to its differentiation into mammal-infective forms.					
31107901	4	131	theme	native	811:816	arg1	mucins					846:851	native and recombinant Gp35/50 kDa mucins	811:851	mucins	846:851	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	8	132	theme	cruzi	2633:2637	arg1	strategies					2661:2670	T. cruzi transmission-blocking strategies	2630:2670	T. cruzi transmission-blocking strategies	2630:2670	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	5	133	from	scaffolds	1573:1581	arg1	determinants					1718:1729	main adhesion determinants	1704:1729	main adhesion determinants for these molecules	1704:1749	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	133	from	scaffolds	1573:1581	arg1	peptide					1513:1519	A solvent-exposed peptide	1495:1519	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans	1495:1683	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	133	from	scaffolds	1573:1581	arg1	peptide					1528:1534	smugS peptide	1522:1534	smugS peptide	1522:1534	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	6	134	theme	synthetic	1791:1799	arg1	derivative					1827:1836	a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative	1789:1836	a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative	1789:1836	Interestingly, exogenous addition of a synthetic Galfβ1-4[Galpβ1-6]GlcNAcα derivative or of oligosaccharides containing this structure impaired the attachment of Dm28c but not of CL Brener epimastigotes to triatomine hindgut tissues; which correlates with the presence of Galf residues on the Gp35/50 kDa mucins' O-glycans on the former but not the latter parasite clone.					
31107901	4	135	theme	recombinant	822:832	arg1	mucins					846:851	native and recombinant Gp35/50 kDa mucins	811:851	mucins	846:851	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	8	136	theme	strategies	2661:2670	arg1	development					2615:2625	the development	2611:2625	the development of T. cruzi transmission-blocking strategies	2611:2670	Most importantly, our findings point to Gp35/50 kDa mucins and/or the Galf biosynthetic pathway, which is absent in mammals and insects, as appealing targets for the development of T. cruzi transmission-blocking strategies.					
31107901	5	137	from	trisaccharide	1615:1627	arg1	determinants					1718:1729	main adhesion determinants	1704:1729	main adhesion determinants for these molecules	1704:1749	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	137	from	trisaccharide	1615:1627	arg1	peptide					1513:1519	A solvent-exposed peptide	1495:1519	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans	1495:1683	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	137	from	trisaccharide	1615:1627	arg1	peptide					1528:1534	smugS peptide	1522:1534	smugS peptide	1522:1534	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	138	link	O-linked	1668:1675	arg1	glycans					1677:1683	their O-linked glycans	1662:1683	their O-linked glycans	1662:1683	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	7	139	theme	novel	2170:2174	arg1	insights					2176:2183	novel insights	2170:2183	novel insights into the mechanisms underlying T. cruzi-triatomine interplay	2170:2244	CONCLUSION/SIGNIFICANCE These results provide novel insights into the mechanisms underlying T. cruzi-triatomine interplay, and indicate that inter-strain variations in the O-glycosylation of Gp35/50 kDa mucins may lead to differences in parasite differentiation and hence, in parasite transmissibility to the mammalian host.					
31107901	5	140	theme	adhesion	1709:1716	arg1	peptide					1513:1519	A solvent-exposed peptide	1495:1519	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans	1495:1683	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	5	140	theme	adhesion	1709:1716	arg1	determinants					1718:1729	main adhesion determinants	1704:1729	main adhesion determinants for these molecules	1704:1749	A solvent-exposed peptide (smugS peptide) from the Gp35/50 kDa mucins protein scaffolds and a branched, Galf-containing trisaccharide (Galfβ1-4[Galpβ1-6]GlcNAcα) from their O-linked glycans were identified as main adhesion determinants for these molecules.					
31107901	4	141	theme	infestans	1371:1379	arg1	ampoules					1388:1395	T. infestans rectal ampoules	1368:1395	T. infestans rectal ampoules in ex vivo binding assays	1368:1421	Experimental evidence supporting this conclusion could be summarized as follows: i) native and recombinant Gp35/50 kDa mucins directly interacted with hindgut tissues from Triatoma infestans, as assessed by indirect immunofluorescence assays; ii) transgenic epimastigotes over-expressing Gp35/50 kDa mucins on their surface coat exhibited improved attachment rates (~2-3 fold) to such tissues as compared to appropriate transgenic controls and/or wild-type counterparts; and iii) certain chemically synthesized compounds derived from Gp35/50 kDa mucins were able to specifically interfere with epimastigote attachment to the inner lining of T. infestans rectal ampoules in ex vivo binding assays, most likely by competing with or directly blocking insect receptor(s).					
31107901	2	142	theme	digestive	354:362	arg1	tract					364:368	the digestive tract	350:368	the digestive tract of the insect	350:382	However, and despite its utmost biological and epidemiological relevance, T. cruzi development inside the digestive tract of the insect remains a poorly understood process.					
31107901	1	143	theme	triatomine	228:237	arg1	vectors					239:245	blood-sucking triatomine vectors	214:245	blood-sucking triatomine vectors	214:245	BACKGROUND Trypanosoma cruzi, the agent of Chagas disease, is a protozoan parasite transmitted to humans by blood-sucking triatomine vectors.					
31987167	10	0	theme	79.4	1630:1633	arg1	%					1634:1634	%	1634:1634	%	1634:1634	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	6	1	theme	Tris-HCl	1058:1065	arg1	100 mmol L-1					1075:1086	100 mmol L-1	1075:1086	100 mmol L-1	1075:1086	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	6	1	theme	Tris-HCl	1058:1065	arg1	buffer					1067:1072	Tris-HCl buffer	1058:1072	Tris-HCl buffer (100 mmol L-1)	1058:1087	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	2	2	theme	protein	283:289	arg1	adsorption					291:300	protein adsorption	283:300	protein adsorption	283:300	For further proteomic studies, however, it is of highly importance to perform protein adsorption in neutral medium.					
31987167	6	3	dep	91	1039:1040	arg1	to					1036:1037	to	1036:1037	to	1036:1037	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	6	4	theme	%	1125:1125	arg1	efficiency					1109:1118	an elution efficiency	1098:1118	an elution efficiency of 82%	1098:1125	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	0	5	theme	Boronic	0:6	arg1	acid					8:11	Boronic acid	0:11	Boronic acid	0:11	Boronic acid modified polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment.					
31987167	1	6	theme	alkaline	184:191	arg1	conditions					193:202	alkaline conditions	184:202	alkaline conditions	184:202	Boronate affinity is widely used for the isolation of glycoproteins at alkaline conditions.					
31987167	10	7	theme	spectrometry	1498:1509	arg1	analysis					1522:1529	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1464:1529	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1464:1529	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	3	8	theme	Ni6PW9	505:510	arg1	hybrid					534:539	a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid	349:539	hybrid	534:539	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	4	9	from	atoms	562:566	arg1	hybrid					575:580	the hybrid	571:580	the hybrid (shortly termed as CPBA-Ni6PW9/SA)	571:615	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	4	10	theme	abundant	546:553	arg1	atoms					562:566	The abundant oxygen atoms	542:566	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA)	542:615	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	10	11	theme	chromatography-tandem	1471:1491	arg1	spectrometry					1498:1509	Liquid chromatography-tandem mass spectrometry	1464:1509	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1464:1529	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	10	11	theme	chromatography-tandem	1471:1491	arg1	LC-MS/MS					1512:1519	LC-MS/MS	1512:1519	LC-MS/MS	1512:1519	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	3	12	theme	•nH2O	498:502	arg1	hybrid					534:539	a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid	349:539	hybrid	534:539	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	9	13	theme	SDS-PAGE	1348:1355	arg1	result					1363:1368	SDS-PAGE assay result	1348:1368	SDS-PAGE assay result	1348:1368	SDS-PAGE assay result indicates that approximately 92% serum albumin is eliminated and high-purity IgG is obtained.					
31987167	8	14	theme	glycoproteins	1316:1328	arg1	enrichment					1302:1311	the enrichment	1298:1311	the enrichment of glycoproteins from human serum	1298:1345	The CPBA-Ni6PW9/SA hybrid is practically applied for the enrichment of glycoproteins from human serum.					
31987167	8	15	theme	human	1335:1339	arg1	serum					1341:1345	human serum	1335:1345	human serum	1335:1345	The CPBA-Ni6PW9/SA hybrid is practically applied for the enrichment of glycoproteins from human serum.					
31987167	7	16	theme	495 mg g-1	1233:1242	arg1	capacity					1221:1228	a maximum adsorption capacity	1200:1228	a maximum adsorption capacity of 495 mg g-1	1200:1242	The adsorption behavior of IgG fits Langmuir adsorption model, offering a maximum adsorption capacity of 495 mg g-1.					
31987167	3	17	theme	-sodium	512:518	arg1	hybrid					534:539	a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid	349:539	hybrid	534:539	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	8	18	theme	CPBA-Ni6PW9/SA	1249:1262	arg1	hybrid					1264:1269	The CPBA-Ni6PW9/SA hybrid	1245:1269	The CPBA-Ni6PW9/SA hybrid	1245:1269	The CPBA-Ni6PW9/SA hybrid is practically applied for the enrichment of glycoproteins from human serum.					
31987167	6	19	theme	adsorption	924:933	arg1	efficiency					935:944	The adsorption efficiency	920:944	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	920:1023	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	0	20	from	environment	100:110	arg1	glycoproteins					75:87	glycoproteins	75:87	glycoproteins at neutral environment	75:110	Boronic acid modified polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment.					
31987167	0	20	from	environment	100:110	arg1	isolation					62:70	the isolation	58:70	the isolation of glycoproteins at neutral environment	58:110	Boronic acid modified polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment.					
31987167	7	21	theme	maximum	1202:1208	arg1	capacity					1221:1228	a maximum adsorption capacity	1200:1228	a maximum adsorption capacity of 495 mg g-1	1200:1242	The adsorption behavior of IgG fits Langmuir adsorption model, offering a maximum adsorption capacity of 495 mg g-1.					
31987167	9	22	theme	serum	1403:1407	arg1	albumin					1409:1415	approximately 92% serum albumin	1385:1415	approximately 92% serum albumin	1385:1415	SDS-PAGE assay result indicates that approximately 92% serum albumin is eliminated and high-purity IgG is obtained.					
31987167	1	23	theme	glycoproteins	167:179	arg1	isolation					154:162	the isolation	150:162	the isolation of glycoproteins	150:179	Boronate affinity is widely used for the isolation of glycoproteins at alkaline conditions.					
31987167	4	24	from	pH	760:761	arg1	environment					744:754	neutral environment	736:754	neutral environment (at pH 7.0)	736:766	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	5	25	from	moiety	773:778	arg1	hybrid					811:816	the hybrid	807:816	the hybrid	807:816	The moiety of sodium alginate (SA) in the hybrid further improves the isolation/enrichment capacity for glycoproteins through hydrophilic interaction.					
31987167	0	26	gly	glycoproteins	75:87	arg1	glycoproteins					75:87	glycoproteins	75:87	glycoproteins at neutral environment	75:110	Boronic acid modified polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment.					
31987167	7	27	theme	adsorption	1173:1182	arg1	model					1184:1188	Langmuir adsorption model	1164:1188	Langmuir adsorption model	1164:1188	The adsorption behavior of IgG fits Langmuir adsorption model, offering a maximum adsorption capacity of 495 mg g-1.					
31987167	3	28	theme	functionalized	418:431	arg1	[Ni6					468:471	i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3	377:476	[Ni6	468:471	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	28	theme	functionalized	418:431	arg1	Tris					478:481	Tris	478:481	Tris	478:481	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	28	theme	functionalized	418:431	arg1	H2O					484:486	H2O	484:486	H2O	484:486	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	10	29	gly	glycoproteins	1597:1609	arg1	glycoproteins					1597:1609	81 glycoproteins	1594:1609	81 glycoproteins	1594:1609	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	0	30	theme	glycoproteins	75:87	arg1	isolation					62:70	the isolation	58:70	the isolation of glycoproteins at neutral environment	58:110	Boronic acid modified polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment.					
31987167	9	31	dep	albumin	1409:1415	arg1	%					1401:1401	%	1401:1401	%	1401:1401	SDS-PAGE assay result indicates that approximately 92% serum albumin is eliminated and high-purity IgG is obtained.					
31987167	3	32	theme	CPBA	412:415	arg1	[Ni6					468:471	i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3	377:476	[Ni6	468:471	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	32	theme	CPBA	412:415	arg1	Tris					478:481	Tris	478:481	Tris	478:481	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	32	theme	CPBA	412:415	arg1	H2O					484:486	H2O	484:486	H2O	484:486	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	4	33	gly	glycoproteins	719:731	arg1	glycoproteins					719:731	glycoproteins	719:731	glycoproteins	719:731	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	4	34	dep	reduce	631:636	arg1	facilitates					679:689	facilitates	679:689	well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0)	674:766	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	3	35	theme	composite	357:365	arg1	hybrid					534:539	a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid	349:539	hybrid	534:539	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	9	36	dep	%	1401:1401	arg1	92					1399:1400	92	1399:1400	92	1399:1400	SDS-PAGE assay result indicates that approximately 92% serum albumin is eliminated and high-purity IgG is obtained.					
31987167	6	37	theme	G	964:964	arg1	hybrid					1018:1023	Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	949:1023	Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	949:1023	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	3	38	theme	acid	406:409	arg1	[Ni6					468:471	i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3	377:476	[Ni6	468:471	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	38	theme	acid	406:409	arg1	Tris					478:481	Tris	478:481	Tris	478:481	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	38	theme	acid	406:409	arg1	H2O					484:486	H2O	484:486	H2O	484:486	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	7	39	theme	IgG	1155:1157	arg1	behavior					1143:1150	The adsorption behavior	1128:1150	The adsorption behavior of IgG	1128:1157	The adsorption behavior of IgG fits Langmuir adsorption model, offering a maximum adsorption capacity of 495 mg g-1.					
31987167	4	40	theme	glycoproteins	719:731	arg1	adsorption					705:714	the selective adsorption	691:714	the selective adsorption of glycoproteins	691:731	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	3	41	theme	polyoxometalate	452:466	arg1	[Ni6					468:471	i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3	377:476	[Ni6	468:471	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	41	theme	polyoxometalate	452:466	arg1	Tris					478:481	Tris	478:481	Tris	478:481	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	41	theme	polyoxometalate	452:466	arg1	H2O					484:486	H2O	484:486	H2O	484:486	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	4	42	theme	neutral	736:742	arg1	environment					744:754	neutral environment	736:754	neutral environment (at pH 7.0)	736:766	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	6	43	theme	buffer	1067:1072	arg1	1.0 mL					1048:1053	1.0 mL	1048:1053	1.0 mL of Tris-HCl buffer (100 mmol L-1)	1048:1087	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	2	44	theme	proteomic	217:225	arg1	studies					227:233	further proteomic studies	209:233	further proteomic studies	209:233	For further proteomic studies, however, it is of highly importance to perform protein adsorption in neutral medium.					
31987167	4	45	dep	hybrid	575:580	arg1	termed					591:596	termed	591:596	termed as CPBA-Ni6PW9/SA	591:614	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	7	46	theme	adsorption	1132:1141	arg1	behavior					1143:1150	The adsorption behavior	1128:1150	The adsorption behavior of IgG	1128:1157	The adsorption behavior of IgG fits Langmuir adsorption model, offering a maximum adsorption capacity of 495 mg g-1.					
31987167	6	47	theme	 by	992:994	arg1	hybrid					1018:1023	Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	949:1023	Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	949:1023	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	10	48	theme	%	1634:1634	arg1	selectivity					1648:1658	79.4% recognition selectivity	1630:1658	79.4% recognition selectivity	1630:1658	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	10	49	theme	Liquid	1464:1469	arg1	spectrometry					1498:1509	Liquid chromatography-tandem mass spectrometry	1464:1509	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1464:1529	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	10	49	theme	Liquid	1464:1469	arg1	LC-MS/MS					1512:1519	LC-MS/MS	1512:1519	LC-MS/MS	1512:1519	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	8	50	gly	glycoproteins	1316:1328	arg1	glycoproteins					1316:1328	glycoproteins	1316:1328	glycoproteins	1316:1328	The CPBA-Ni6PW9/SA hybrid is practically applied for the enrichment of glycoproteins from human serum.					
31987167	1	51	used	used	141:144	arg2	affinity					122:129	Boronate affinity	113:129	Boronate affinity	113:129	Boronate affinity is widely used for the isolation of glycoproteins at alkaline conditions.					
31987167	10	52	theme	mass	1493:1496	arg1	spectrometry					1498:1509	Liquid chromatography-tandem mass spectrometry	1464:1509	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis	1464:1529	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	10	52	theme	mass	1493:1496	arg1	LC-MS/MS					1512:1519	LC-MS/MS	1512:1519	LC-MS/MS	1512:1519	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31987167	2	53	theme	neutral	305:311	arg1	medium					313:318	neutral medium	305:318	neutral medium	305:318	For further proteomic studies, however, it is of highly importance to perform protein adsorption in neutral medium.					
31987167	5	54	theme	alginate	790:797	arg1	moiety					773:778	The moiety	769:778	The moiety of sodium alginate (SA) in the hybrid	769:816	The moiety of sodium alginate (SA) in the hybrid further improves the isolation/enrichment capacity for glycoproteins through hydrophilic interaction.					
31987167	3	55	theme	alginate	520:527	arg1	hybrid					534:539	a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid	349:539	hybrid	534:539	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	9	56	theme	assay	1357:1361	arg1	result					1363:1368	SDS-PAGE assay result	1348:1368	SDS-PAGE assay result	1348:1368	SDS-PAGE assay result indicates that approximately 92% serum albumin is eliminated and high-purity IgG is obtained.					
31987167	6	57	theme	elution	1101:1107	arg1	efficiency					1109:1118	an elution efficiency	1098:1118	an elution efficiency of 82%	1098:1125	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	4	58	theme	oxygen	555:560	arg1	atoms					562:566	The abundant oxygen atoms	542:566	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA)	542:615	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	1	59	gly	glycoproteins	167:179	arg1	glycoproteins					167:179	glycoproteins	167:179	glycoproteins	167:179	Boronate affinity is widely used for the isolation of glycoproteins at alkaline conditions.					
31987167	8	60	from	serum	1341:1345	arg1	enrichment					1302:1311	the enrichment	1298:1311	the enrichment of glycoproteins from human serum	1298:1345	The CPBA-Ni6PW9/SA hybrid is practically applied for the enrichment of glycoproteins from human serum.					
31987167	3	61	theme	SA	530:531	arg1	hybrid					534:539	a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid	349:539	hybrid	534:539	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	7	62	theme	adsorption	1210:1219	arg1	capacity					1221:1228	a maximum adsorption capacity	1200:1228	a maximum adsorption capacity of 495 mg g-1	1200:1242	The adsorption behavior of IgG fits Langmuir adsorption model, offering a maximum adsorption capacity of 495 mg g-1.					
31987167	0	63	theme	neutral	92:98	arg1	environment					100:110	neutral environment	92:110	neutral environment	92:110	Boronic acid modified polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment.					
31987167	5	64	theme	sodium	783:788	arg1	SA					800:801	SA	800:801	SA	800:801	The moiety of sodium alginate (SA) in the hybrid further improves the isolation/enrichment capacity for glycoproteins through hydrophilic interaction.					
31987167	5	64	theme	sodium	783:788	arg1	alginate					790:797	sodium alginate	783:797	sodium alginate (SA)	783:802	The moiety of sodium alginate (SA) in the hybrid further improves the isolation/enrichment capacity for glycoproteins through hydrophilic interaction.					
31987167	3	65	theme	novel	351:355	arg1	hybrid					534:539	a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid	349:539	hybrid	534:539	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	0	66	from	isolation	62:70	arg1	environment					100:110	neutral environment	92:110	neutral environment	92:110	Boronic acid modified polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment.					
31987167	5	67	theme	isolation/enrichment	839:858	arg1	capacity					860:867	the isolation/enrichment capacity	835:867	the isolation/enrichment capacity for glycoproteins	835:885	The moiety of sodium alginate (SA) in the hybrid further improves the isolation/enrichment capacity for glycoproteins through hydrophilic interaction.					
31987167	4	68	theme	moiety	660:665	arg1	value					646:650	the pKa value	638:650	the pKa value of CPBA moiety	638:665	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	6	69	theme	CPBA-Ni6PW9/SA	1003:1016	arg1	hybrid					1018:1023	Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	949:1023	Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	949:1023	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	4	70	dep	termed	591:596	arg1	shortly					583:589	shortly	583:589	shortly	583:589	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	6	71	dep	G	964:964	arg1	100 μg mL-1					980:990	100 μg mL-1	980:990	100 μg mL-1	980:990	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	6	71	dep	G	964:964	arg1	1.0 mL					972:977	1.0 mL	972:977	1.0 mL	972:977	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	6	71	dep	G	964:964	arg1	IgG					967:969	IgG	967:969	IgG	967:969	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	3	72	theme	nickel-substituted	433:450	arg1	[Ni6					468:471	i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3	377:476	[Ni6	468:471	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	72	theme	nickel-substituted	433:450	arg1	Tris					478:481	Tris	478:481	Tris	478:481	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	72	theme	nickel-substituted	433:450	arg1	H2O					484:486	H2O	484:486	H2O	484:486	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	73	theme	i.e.	377:380	arg1	[Ni6					468:471	i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3	377:476	[Ni6	468:471	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	73	theme	i.e.	377:380	arg1	Tris					478:481	Tris	478:481	Tris	478:481	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	73	theme	i.e.	377:380	arg1	H2O					484:486	H2O	484:486	H2O	484:486	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	4	74	theme	pKa	642:644	arg1	value					646:650	the pKa value	638:650	the pKa value of CPBA moiety	638:665	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	0	75	theme	polyoxometalate-alginate	22:45	arg1	hybrid					47:52	polyoxometalate-alginate hybrid	22:52	polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment	22:110	Boronic acid modified polyoxometalate-alginate hybrid for the isolation of glycoproteins at neutral environment.					
31987167	1	76	theme	Boronate	113:120	arg1	affinity					122:129	Boronate affinity	113:129	Boronate affinity	113:129	Boronate affinity is widely used for the isolation of glycoproteins at alkaline conditions.					
31987167	7	77	theme	Langmuir	1164:1171	arg1	adsorption					1173:1182	Langmuir adsorption	1164:1182	Langmuir adsorption model	1164:1188	The adsorption behavior of IgG fits Langmuir adsorption model, offering a maximum adsorption capacity of 495 mg g-1.					
31987167	3	78	theme	material	367:374	arg1	hybrid					534:539	a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid	349:539	hybrid	534:539	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	9	79	theme	high-purity	1435:1445	arg1	IgG					1447:1449	high-purity IgG	1435:1449	high-purity IgG	1435:1449	SDS-PAGE assay result indicates that approximately 92% serum albumin is eliminated and high-purity IgG is obtained.					
31987167	3	80	dep	hybrid	534:539	arg1	[Ni6					468:471	i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3	377:476	[Ni6	468:471	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	80	dep	hybrid	534:539	arg1	Tris					478:481	Tris	478:481	Tris	478:481	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	80	dep	hybrid	534:539	arg1	H2O					484:486	H2O	484:486	H2O	484:486	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	6	81	theme	Immunoglobulin	949:962	arg1	G					964:964	Immunoglobulin G	949:964	Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	949:1023	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	5	82	gly	glycoproteins	873:885	arg1	glycoproteins					873:885	glycoproteins	873:885	glycoproteins	873:885	The moiety of sodium alginate (SA) in the hybrid further improves the isolation/enrichment capacity for glycoproteins through hydrophilic interaction.					
31987167	4	83	theme	CPBA	655:658	arg1	moiety					660:665	CPBA moiety	655:665	CPBA moiety	655:665	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	3	84	theme	4-carboxyphenylboronic	383:404	arg1	[Ni6					468:471	i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3	377:476	[Ni6	468:471	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	84	theme	4-carboxyphenylboronic	383:404	arg1	Tris					478:481	Tris	478:481	Tris	478:481	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	3	84	theme	4-carboxyphenylboronic	383:404	arg1	H2O					484:486	H2O	484:486	H2O	484:486	For this purpose, we report a novel composite material, i.e., 4-carboxyphenylboronic acid (CPBA) functionalized nickel-substituted polyoxometalate [Ni6(en)3(Tris)(H2O)2(PW9O34)]•nH2O (Ni6PW9)-sodium alginate (SA) hybrid.					
31987167	6	85	theme	hybrid	1018:1023	arg1	efficiency					935:944	The adsorption efficiency	920:944	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	920:1023	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	2	86	theme	further	209:215	arg1	studies					227:233	further proteomic studies	209:233	further proteomic studies	209:233	For further proteomic studies, however, it is of highly importance to perform protein adsorption in neutral medium.					
31987167	5	87	theme	hydrophilic	895:905	arg1	interaction					907:917	hydrophilic interaction	895:917	hydrophilic interaction	895:917	The moiety of sodium alginate (SA) in the hybrid further improves the isolation/enrichment capacity for glycoproteins through hydrophilic interaction.					
31987167	6	88	theme	1.0 mg	996:1001	arg1	hybrid					1018:1023	Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	949:1023	Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid	949:1023	The adsorption efficiency of Immunoglobulin G (IgG, 1.0 mL, 100 μg mL-1) by 1.0 mg CPBA-Ni6PW9/SA hybrid reaches up to 91%, and 1.0 mL of Tris-HCl buffer (100 mmol L-1) provides an elution efficiency of 82%.					
31987167	4	89	theme	selective	695:703	arg1	adsorption					705:714	the selective adsorption	691:714	the selective adsorption of glycoproteins	691:731	The abundant oxygen atoms in the hybrid (shortly termed as CPBA-Ni6PW9/SA) significantly reduce the pKa value of CPBA moiety, which well facilitates the selective adsorption of glycoproteins at neutral environment (at pH 7.0).					
31987167	10	90	theme	recognition	1636:1646	arg1	selectivity					1648:1658	79.4% recognition selectivity	1630:1658	79.4% recognition selectivity	1630:1658	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis clearly demonstrated that after enriching with CPBA-Ni6PW9/SA, 81 glycoproteins are identified and 79.4% recognition selectivity is achieved.					
31052187	6	0	theme	fecal	1198:1202	arg1	concentration					1213:1225	fecal butyrate concentration	1198:1225	fecal butyrate concentration	1198:1225	The HAMSB diet also substantially increased fecal butyrate concentration and the pool, compared with the other diets.					
31052187	0	1	theme	Meat	172:175	arg1	Diet					177:180	A High-Protein Meat Diet	157:180	A High-Protein Meat Diet	157:180	High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet.					
31052187	4	2	theme	cecum	851:855	arg1	digesta					857:863	cecum digesta	851:863	cecum digesta	851:863	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	8	3	theme	colonic	1430:1436	arg1	expression					1471:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	0	4	theme	Intestinal	66:75	arg1	Fermentation					77:88	Large Intestinal Fermentation	60:88	Large Intestinal Fermentation	60:88	High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet.					
31052187	1	5	theme	resistant	292:300	arg1	RS					310:311	RS	310:311	RS	310:311	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	1	5	theme	resistant	292:300	arg1	starch					302:307	resistant starch	292:307	resistant starch (RS)	292:312	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	0	6	theme	High-Protein	159:170	arg1	Diet					177:180	A High-Protein Meat Diet	157:180	A High-Protein Meat Diet	157:180	High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet.					
31052187	2	7	theme	negative	565:572	arg1	effects					574:580	the negative effects	561:580	the negative effects of a high-protein meat diet (HPM)	561:614	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	5	8	theme	HAMSB	1057:1061	arg1	diets					1063:1067	The HAMS and HAMSB diets	1044:1067	The HAMS and HAMSB diets	1044:1067	The HAMS and HAMSB diets shifted the fecal fermentation pattern from protein towards carbohydrate metabolism.					
31052187	8	9	theme	reduced	1422:1428	arg1	expression					1471:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	0	10	theme	Microbial	91:99	arg1	Composition					101:111	Microbial Composition	91:111	Microbial Composition	91:111	High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet.					
31052187	5	11	theme	fermentation	1087:1098	arg1	pattern					1100:1106	the fecal fermentation pattern	1077:1106	the fecal fermentation pattern from protein	1077:1119	The HAMS and HAMSB diets shifted the fecal fermentation pattern from protein towards carbohydrate metabolism.					
31052187	9	12	theme	high-meat	1665:1673	arg1	diet					1675:1678	a high-meat diet	1663:1678	a high-meat diet	1663:1678	Overall, HAMSB consumption showed the most potential for limiting the negative effects of a high-meat diet.					
31052187	8	13	theme	O6MeG	1552:1556	arg1	levels					1565:1570	the colonic O6MeG adduct levels	1540:1570	the colonic O6MeG adduct levels	1540:1570	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	2	14	theme	CRC	706:708	arg1	pattern					642:648	fermentation pattern	629:648	fermentation pattern	629:648	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	14	theme	CRC	706:708	arg1	biomarkers					692:701	colonic biomarkers	684:701	colonic biomarkers of CRC	684:708	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	14	theme	CRC	706:708	arg1	composition					667:677	cecal microbial composition	651:677	cecal microbial composition	651:677	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	1	15	theme	colorectal	235:244	arg1	CRC					254:256	CRC	254:256	CRC	254:256	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	1	15	theme	colorectal	235:244	arg1	cancer					246:251	colorectal cancer	235:251	colorectal cancer (CRC)	235:257	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	2	16	theme	maize	440:444	arg1	HAMS					454:457	HAMS	454:457	HAMS	454:457	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	16	theme	maize	440:444	arg1	starch					446:451	high-amylose maize starch	427:451	high-amylose maize starch (HAMS)	427:458	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	8	17	theme	oncogenic	1438:1446	arg1	expression					1471:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	8	18	theme	adduct	1558:1563	arg1	levels					1565:1570	the colonic O6MeG adduct levels	1540:1570	the colonic O6MeG adduct levels	1540:1570	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	4	19	theme	gene	927:930	arg1	expression					932:941	gene expression	927:941	gene expression (oncogenic microRNA)	927:962	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	4	19	theme	gene	927:930	arg1	microRNA					954:961	oncogenic microRNA	944:961	oncogenic microRNA	944:961	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	1	20	theme	cancer	246:251	arg1	risk					227:230	the risk	223:230	the risk of colorectal cancer (CRC)	223:257	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	2	21	theme	high-amylose	427:438	arg1	HAMS					454:457	HAMS	454:457	HAMS	454:457	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	21	theme	high-amylose	427:438	arg1	starch					446:451	high-amylose maize starch	427:451	high-amylose maize starch (HAMS)	427:458	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	5	22	theme	carbohydrate	1129:1140	arg1	metabolism					1142:1151	carbohydrate metabolism	1129:1151	carbohydrate metabolism	1129:1151	The HAMS and HAMSB diets shifted the fecal fermentation pattern from protein towards carbohydrate metabolism.					
31052187	2	23	theme	organocatalytic	526:540	arg1	route-could					542:552	an organocatalytic route-could	523:552	an organocatalytic route-could	523:552	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	5	24	theme	HAMS	1048:1051	arg1	diets					1063:1067	The HAMS and HAMSB diets	1044:1067	The HAMS and HAMSB diets	1044:1067	The HAMS and HAMSB diets shifted the fecal fermentation pattern from protein towards carbohydrate metabolism.					
31052187	2	25	theme	pattern	642:648	arg1	terms					620:624	terms	620:624	terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC	620:708	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	6	26	theme	HAMSB	1158:1162	arg1	diet					1164:1167	The HAMSB diet	1154:1167	The HAMSB diet	1154:1167	The HAMSB diet also substantially increased fecal butyrate concentration and the pool, compared with the other diets.					
31052187	8	27	theme	diet-induced	1512:1523	arg1	differences					1525:1535	no significant diet-induced differences	1497:1535	no significant diet-induced differences in the colonic O6MeG adduct levels	1497:1570	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	2	28	theme	high-protein	587:598	arg1	HPM					611:613	HPM	611:613	HPM	611:613	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	28	theme	high-protein	587:598	arg1	diet					605:608	a high-protein meat diet	585:608	a high-protein meat diet (HPM)	585:614	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	29	theme	fermentation	629:640	arg1	pattern					642:648	fermentation pattern	629:648	fermentation pattern	629:648	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	9	30	theme	HAMSB	1582:1586	arg1	consumption					1588:1598	HAMSB consumption	1582:1598	HAMSB consumption	1582:1598	Overall, HAMSB consumption showed the most potential for limiting the negative effects of a high-meat diet.					
31052187	6	31	theme	other	1259:1263	arg1	diets					1265:1269	the other diets	1255:1269	the other diets	1255:1269	The HAMSB diet also substantially increased fecal butyrate concentration and the pool, compared with the other diets.					
31052187	0	32	theme	High-Amylose	0:11	arg1	Maize					13:17	High-Amylose Maize	0:17	High-Amylose Maize	0:17	High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet.					
31052187	2	33	theme	-produced	510:518	arg1	HAMSB					504:508	HAMSB	504:508	HAMSB	504:508	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	33	theme	-produced	510:518	arg1	starch					496:501	butyrylated high-amylose maize starch	465:501	butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could	465:552	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	3	34	theme	starch	774:779	arg1	%					759:759	10%	757:759	10% of the maize starch	757:779	Rats were fed a HPM diet or an HPM diet where 10% of the maize starch was substituted with either HAPS, HAMS, or HAMSB, for 4 weeks.					
31052187	3	34	theme	starch	774:779	arg1	starch					774:779	the maize starch	764:779	the maize starch	764:779	Rats were fed a HPM diet or an HPM diet where 10% of the maize starch was substituted with either HAPS, HAMS, or HAMSB, for 4 weeks.					
31052187	2	35	theme	study	361:365	arg1	aim					349:351	The aim	345:351	The aim of this study	345:365	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	0	36	theme	Oncogenic	118:126	arg1	Expression					134:143	Oncogenic miRNA Expression	118:143	Oncogenic miRNA Expression	118:143	High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet.					
31052187	4	37	dep	biochemical	903:913	arg1	analysis					1034:1041	analysis	1034:1041	analysis	1034:1041	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	2	38	theme	composition	667:677	arg1	terms					620:624	terms	620:624	terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC	620:708	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	1	39	theme	High	183:186	arg1	intake					197:202	High red meat intake	183:202	High red meat intake	183:202	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	1	40	dep	such	284:287	arg1	as					289:290	as	289:290	as	289:290	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	4	41	theme	O6-methyl-2-deoxyguanosine	991:1016	arg1	immuno-histochemical					969:988	immuno-histochemical	969:988	immuno-histochemical	969:988	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	4	41	theme	O6-methyl-2-deoxyguanosine	991:1016	arg1	adduct					1026:1031	O6-methyl-2-deoxyguanosine (O6MeG) adduct	991:1031	O6-methyl-2-deoxyguanosine (O6MeG) adduct	991:1031	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	7	42	theme	specific	1346:1353	arg1	manner					1355:1360	a diet specific manner	1339:1360	a diet specific manner	1339:1360	All three RS treatments altered the cecal microbial composition in a diet specific manner.					
31052187	3	43	theme	maize	768:772	arg1	starch					774:779	the maize starch	764:779	the maize starch	764:779	Rats were fed a HPM diet or an HPM diet where 10% of the maize starch was substituted with either HAPS, HAMS, or HAMSB, for 4 weeks.					
31052187	2	44	theme	microbial	657:665	arg1	composition					667:677	cecal microbial composition	651:677	cecal microbial composition	651:677	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	1	45	theme	red	188:190	arg1	intake					197:202	High red meat intake	183:202	High red meat intake	183:202	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	2	46	theme	potato	405:410	arg1	HAPS					420:423	HAPS	420:423	HAPS	420:423	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	46	theme	potato	405:410	arg1	starch					412:417	high-amylose potato starch	392:417	high-amylose potato starch (HAPS)	392:424	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	8	47	theme	significant	1500:1510	arg1	differences					1525:1535	no significant diet-induced differences	1497:1535	no significant diet-induced differences in the colonic O6MeG adduct levels	1497:1570	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	8	48	theme	cluster	1457:1463	arg1	expression					1471:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	5	49	from	protein	1113:1119	arg1	pattern					1100:1106	the fecal fermentation pattern	1077:1106	the fecal fermentation pattern from protein	1077:1119	The HAMS and HAMSB diets shifted the fecal fermentation pattern from protein towards carbohydrate metabolism.					
31052187	2	50	theme	cecal	651:655	arg1	composition					667:677	cecal microbial composition	651:677	cecal microbial composition	651:677	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	1	51	theme	meat	192:195	arg1	intake					197:202	High red meat intake	183:202	High red meat intake	183:202	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	2	52	theme	high-amylose	392:403	arg1	HAPS					420:423	HAPS	420:423	HAPS	420:423	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	52	theme	high-amylose	392:403	arg1	starch					412:417	high-amylose potato starch	392:417	high-amylose potato starch (HAPS)	392:424	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	7	53	theme	RS	1282:1283	arg1	treatments					1285:1294	All three RS treatments	1272:1294	All three RS treatments	1272:1294	All three RS treatments altered the cecal microbial composition in a diet specific manner.					
31052187	0	54	theme	miRNA	128:132	arg1	Expression					134:143	Oncogenic miRNA Expression	118:143	Oncogenic miRNA Expression	118:143	High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet.					
31052187	8	55	from	differences	1525:1535	arg1	levels					1565:1570	the colonic O6MeG adduct levels	1540:1570	the colonic O6MeG adduct levels	1540:1570	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	8	56	theme	miRNA	1465:1469	arg1	expression					1471:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	1	57	theme	dietary	268:274	arg1	fibers					276:281	dietary fibers	268:281	dietary fibers	268:281	High red meat intake is associated with the risk of colorectal cancer (CRC), whereas dietary fibers, such as resistant starch (RS) seemed to protect against CRC.					
31052187	2	58	theme	maize	490:494	arg1	HAMSB					504:508	HAMSB	504:508	HAMSB	504:508	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	58	theme	maize	490:494	arg1	starch					496:501	butyrylated high-amylose maize starch	465:501	butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could	465:552	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	7	59	theme	diet	1341:1344	arg1	manner					1355:1360	a diet specific manner	1339:1360	a diet specific manner	1339:1360	All three RS treatments altered the cecal microbial composition in a diet specific manner.					
31052187	8	60	theme	miR17-92	1448:1455	arg1	expression					1471:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	the reduced colonic oncogenic miR17-92 cluster miRNA expression	1418:1480	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	9	61	theme	negative	1643:1650	arg1	effects					1652:1658	the negative effects	1639:1658	the negative effects of a high-meat diet	1639:1678	Overall, HAMSB consumption showed the most potential for limiting the negative effects of a high-meat diet.					
31052187	0	62	theme	Butyrylated	32:42	arg1	Starch					44:49	Butyrylated Starch	32:49	Butyrylated Starch	32:49	High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet.					
31052187	2	63	theme	high-amylose	477:488	arg1	HAMSB					504:508	HAMSB	504:508	HAMSB	504:508	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	63	theme	high-amylose	477:488	arg1	starch					496:501	butyrylated high-amylose maize starch	465:501	butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could	465:552	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	64	theme	diet	605:608	arg1	effects					574:580	the negative effects	561:580	the negative effects of a high-protein meat diet (HPM)	561:614	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	3	65	theme	HPM	727:729	arg1	diet					731:734	a HPM diet	725:734	a HPM diet	725:734	Rats were fed a HPM diet or an HPM diet where 10% of the maize starch was substituted with either HAPS, HAMS, or HAMSB, for 4 weeks.					
31052187	8	66	theme	preventive	1389:1398	arg1	effects					1400:1406	CRC preventive effects	1385:1406	CRC preventive effects	1385:1406	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	2	67	theme	butyrylated	465:475	arg1	HAMSB					504:508	HAMSB	504:508	HAMSB	504:508	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	67	theme	butyrylated	465:475	arg1	starch					496:501	butyrylated high-amylose maize starch	465:501	butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could	465:552	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	4	68	theme	O6MeG	1019:1023	arg1	immuno-histochemical					969:988	immuno-histochemical	969:988	immuno-histochemical	969:988	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	4	68	theme	O6MeG	1019:1023	arg1	adduct					1026:1031	O6-methyl-2-deoxyguanosine (O6MeG) adduct	991:1031	O6-methyl-2-deoxyguanosine (O6MeG) adduct	991:1031	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	2	69	theme	meat	600:603	arg1	HPM					611:613	HPM	611:613	HPM	611:613	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	69	theme	meat	600:603	arg1	diet					605:608	a high-protein meat diet	585:608	a high-protein meat diet (HPM)	585:614	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	2	70	theme	biomarkers	692:701	arg1	terms					620:624	terms	620:624	terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC	620:708	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	7	71	theme	cecal	1308:1312	arg1	composition					1324:1334	the cecal microbial composition	1304:1334	the cecal microbial composition	1304:1334	All three RS treatments altered the cecal microbial composition in a diet specific manner.					
31052187	9	72	theme	diet	1675:1678	arg1	effects					1652:1658	the negative effects	1639:1658	the negative effects of a high-meat diet	1639:1678	Overall, HAMSB consumption showed the most potential for limiting the negative effects of a high-meat diet.					
31052187	2	73	theme	colonic	684:690	arg1	biomarkers					692:701	colonic biomarkers	684:701	colonic biomarkers of CRC	684:708	The aim of this study was to determine whether high-amylose potato starch (HAPS), high-amylose maize starch (HAMS), and butyrylated high-amylose maize starch (HAMSB)-produced by an organocatalytic route-could oppose the negative effects of a high-protein meat diet (HPM), in terms of fermentation pattern, cecal microbial composition, and colonic biomarkers of CRC.					
31052187	0	74	theme	Large	60:64	arg1	Fermentation					77:88	Large Intestinal Fermentation	60:88	Large Intestinal Fermentation	60:88	High-Amylose Maize, Potato, and Butyrylated Starch Modulate Large Intestinal Fermentation, Microbial Composition, and Oncogenic miRNA Expression in Rats Fed A High-Protein Meat Diet.					
31052187	7	75	theme	microbial	1314:1322	arg1	composition					1324:1334	the cecal microbial composition	1304:1334	the cecal microbial composition	1304:1334	All three RS treatments altered the cecal microbial composition in a diet specific manner.					
31052187	4	76	theme	oncogenic	944:952	arg1	expression					932:941	gene expression	927:941	gene expression (oncogenic microRNA)	927:962	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	4	76	theme	oncogenic	944:952	arg1	microRNA					954:961	oncogenic microRNA	944:961	oncogenic microRNA	944:961	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	4	77	theme	colonic	870:876	arg1	tissue					878:883	colonic tissue	870:883	colonic tissue	870:883	Feces, cecum digesta, and colonic tissue were obtained for biochemical, microbial, gene expression (oncogenic microRNA), and immuno-histochemical (O6-methyl-2-deoxyguanosine (O6MeG) adduct) analysis.					
31052187	8	78	theme	CRC	1385:1387	arg1	effects					1400:1406	CRC preventive effects	1385:1406	CRC preventive effects	1385:1406	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	6	79	theme	butyrate	1204:1211	arg1	concentration					1213:1225	fecal butyrate concentration	1198:1225	fecal butyrate concentration	1198:1225	The HAMSB diet also substantially increased fecal butyrate concentration and the pool, compared with the other diets.					
31052187	8	80	theme	colonic	1544:1550	arg1	levels					1565:1570	the colonic O6MeG adduct levels	1540:1570	the colonic O6MeG adduct levels	1540:1570	HAPS and HAMSB showed CRC preventive effects, based on the reduced colonic oncogenic miR17-92 cluster miRNA expression, but there was no significant diet-induced differences in the colonic O6MeG adduct levels.					
31052187	5	81	theme	fecal	1081:1085	arg1	pattern					1100:1106	the fecal fermentation pattern	1077:1106	the fecal fermentation pattern from protein	1077:1119	The HAMS and HAMSB diets shifted the fecal fermentation pattern from protein towards carbohydrate metabolism.					
31052187	9	82	theme	most	1611:1614	arg1	potential					1616:1624	the most potential	1607:1624	the most potential for limiting the negative effects of a high-meat diet	1607:1678	Overall, HAMSB consumption showed the most potential for limiting the negative effects of a high-meat diet.					
31052187	3	83	theme	HPM	742:744	arg1	diet					746:749	an HPM diet	739:749	an HPM diet where 10% of the maize starch was substituted with either HAPS, HAMS, or HAMSB	739:828	Rats were fed a HPM diet or an HPM diet where 10% of the maize starch was substituted with either HAPS, HAMS, or HAMSB, for 4 weeks.					
29575695	1	0	theme	due	282:284	arg1	tissues					234:240	biologic tissues	225:240	biologic tissues	225:240	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	6	1	theme	obvious	1292:1298	arg1	behavior					1312:1319	no obvious degradation behavior	1289:1319	no obvious degradation behavior	1289:1319	The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property.					
29575695	3	2	theme	composite	799:807	arg1	hydrogel					809:816	the prepared composite hydrogel	786:816	the prepared composite hydrogel	786:816	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	3	dep	in	753:754	arg1	vitro					756:760	vitro	756:760	vitro	756:760	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	9	4	theme	scaffolds	1764:1772	arg1	development					1740:1750	development	1740:1750	development	1740:1750	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	9	4	theme	scaffolds	1764:1772	arg1	design					1729:1734	design	1729:1734	design	1729:1734	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	9	5	theme	nerve	1778:1782	arg1	application					1803:1813	nerve tissue engineering application	1778:1813	nerve tissue engineering application	1778:1813	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	6	6	theme	ingredient	1271:1280	arg1	alteration					1252:1261	the concentration alteration	1234:1261	the concentration alteration of each ingredient	1234:1280	The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property.					
29575695	6	7	theme	physicochemical	1361:1375	arg1	property					1377:1384	a controllable physicochemical property	1346:1384	a controllable physicochemical property	1346:1384	The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property.					
29575695	3	8	theme	physicochemical	636:650	arg1	morphology					673:682	morphology	673:682	morphology	673:682	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	8	theme	physicochemical	636:650	arg1	component					715:723	component	715:723	component	715:723	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	8	theme	physicochemical	636:650	arg1	porosity					685:692	porosity	685:692	porosity	685:692	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	8	theme	physicochemical	636:650	arg1	behavior					774:781	in vitro degradation behavior	753:781	in vitro degradation behavior of the prepared composite hydrogel	753:816	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	8	theme	physicochemical	636:650	arg1	properties					737:746	mechanical properties	726:746	mechanical properties	726:746	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	8	theme	physicochemical	636:650	arg1	behaviors					704:712	swelling behaviors	695:712	swelling behaviors	695:712	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	8	theme	physicochemical	636:650	arg1	properties					652:661	physicochemical properties	636:661	physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel	636:816	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	9	9	theme	engineering	1791:1801	arg1	application					1803:1813	nerve tissue engineering application	1778:1813	nerve tissue engineering application	1778:1813	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	2	10	theme	first	612:616	arg1	time					618:621	the first time	608:621	the first time	608:621	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	6	11	theme	controllable	1348:1359	arg1	property					1377:1384	a controllable physicochemical property	1346:1384	a controllable physicochemical property	1346:1384	The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property.					
29575695	1	12	theme	drug	243:246	arg1	delivery					248:255	drug delivery	243:255	drug delivery	243:255	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	8	13	theme	basic	1572:1576	arg1	MBP					1587:1589	MBP	1587:1589	MBP	1587:1589	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	8	13	theme	basic	1572:1576	arg1	protein					1578:1584	myelin basic protein	1565:1584	myelin basic protein (MBP)	1565:1590	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	7	14	theme	Schwann	1502:1508	arg1	cells					1510:1514	Schwann cells	1502:1514	Schwann cells	1502:1514	The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells.					
29575695	11	15	dep	Res	1862:1864	arg1	106A					1874:1877	106A	1874:1877	106A	1874:1877	J Biomed Mater Res Part A: 106A: 1951-1964, 2018.					
29575695	11	15	dep	Res	1862:1864	arg1	A					1871:1871	Part A	1866:1871	J Biomed Mater Res Part A: 106A: 1951-1964, 2018.	1847:1895	J Biomed Mater Res Part A: 106A: 1951-1964, 2018.					
29575695	7	16	theme	cells	1402:1406	arg1	culture					1391:1397	The culture	1387:1397	The culture of cells	1387:1406	The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells.					
29575695	5	17	theme	prepared	1003:1010	arg1	hydrogels					1036:1044	the prepared PAM/GO/Gel/SA composite hydrogels	999:1044	the prepared PAM/GO/Gel/SA composite hydrogels	999:1044	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	5	18	theme	composite	1026:1034	arg1	hydrogels					1036:1044	the prepared PAM/GO/Gel/SA composite hydrogels	999:1044	the prepared PAM/GO/Gel/SA composite hydrogels	999:1044	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	1	19	theme	regenerative	356:367	arg1	medicine					369:376	regenerative medicine	356:376	regenerative medicine	356:376	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	1	20	theme	synthetic	181:189	arg1	polymers					191:198	natural or synthetic polymers	170:198	natural or synthetic polymers	170:198	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	1	21	theme	Various	142:148	arg1	hydrogels					150:158	Various hydrogels	142:158	Various hydrogels made from natural or synthetic polymers	142:198	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	0	22	theme	cells	128:132	arg1	growth					134:139	Schwann cells growth	120:139	Schwann cells growth	120:139	Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth.					
29575695	1	23	theme	natural	170:176	arg1	polymers					191:198	natural or synthetic polymers	170:198	natural or synthetic polymers	170:198	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	2	24	theme	radical	581:587	arg1	polymerization					589:602	in situ free radical polymerization	568:602	in situ free radical polymerization	568:602	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	4	25	theme	composite	857:865	arg1	hydrogel					867:874	the composite hydrogel	853:874	the composite hydrogel	853:874	The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated.					
29575695	2	26	theme	composite	403:411	arg1	hydrogel					413:420	a composite hydrogel	401:420	a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration	401:543	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	1	27	theme	good	295:298	arg1	biocompatibility					300:315	their good biocompatibility	289:315	their good biocompatibility	289:315	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	4	28	theme	related	923:929	arg1	mechanism					941:949	the related molecular mechanism	919:949	the related molecular mechanism	919:949	The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated.					
29575695	10	29	dep	©	1816:1816	arg1	Inc.					1842:1845	Inc.	1842:1845	Inc.	1842:1845	© 2018 Wiley Periodicals, Inc.					
29575695	0	30	theme	polyacrylamide/graphene	16:38	arg1	hydrogel					80:87	polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel	16:87	polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity	16:104	Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth.					
29575695	5	31	theme	color	1066:1070	arg1	appearance					1072:1081	different color appearance	1056:1081	different color appearance	1056:1081	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	5	31	theme	color	1066:1070	arg1	function					1090:1097	the function	1086:1097	the function of component variations	1086:1121	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	6	32	theme	swelling	1160:1167	arg1	ratio					1169:1173	swelling ratio	1160:1173	swelling ratio	1160:1173	The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property.					
29575695	3	33	theme	mechanical	726:735	arg1	properties					737:746	mechanical properties	726:746	mechanical properties	726:746	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	8	34	theme	expression	1526:1535	arg1	levels					1537:1542	The gene expression levels	1517:1542	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5	1517:1621	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	8	34	theme	expression	1526:1535	arg1	higher					1628:1633	higher	1628:1633	higher	1628:1633	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	2	35	theme	oxide/gelatin/sodium	452:471	arg1	PAM/GO/Gel/SA					483:495	PAM/GO/Gel/SA	483:495	PAM/GO/Gel/SA	483:495	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	2	35	theme	oxide/gelatin/sodium	452:471	arg1	alginate					473:480	polyacrylamide/graphene oxide/gelatin/sodium alginate	428:480	polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA)	428:496	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	0	36	theme	alginate	61:68	arg1	hydrogel					80:87	polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel	16:87	polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity	16:104	Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth.					
29575695	9	37	theme	theoretical	1686:1696	arg1	basis					1715:1719	important theoretical and experimental basis	1676:1719	important theoretical and experimental basis for the design and development of hydrogel scaffolds	1676:1772	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	2	38	theme	nerve	526:530	arg1	regeneration					532:543	peripheral nerve regeneration	515:543	peripheral nerve regeneration	515:543	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	0	39	theme	hydrogel	80:87	arg1	Construction					0:11	Construction	0:11	Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth.	0:140	Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth.					
29575695	11	40	dep	106A	1874:1877	arg1	1951-1964					1880:1888	1951-1964	1880:1888	1951-1964	1880:1888	J Biomed Mater Res Part A: 106A: 1951-1964, 2018.					
29575695	9	41	theme	experimental	1702:1713	arg1	basis					1715:1719	important theoretical and experimental basis	1676:1719	important theoretical and experimental basis for the design and development of hydrogel scaffolds	1676:1772	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	3	42	theme	in	753:754	arg1	behavior					774:781	in vitro degradation behavior	753:781	in vitro degradation behavior of the prepared composite hydrogel	753:816	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	2	43	dep	in	568:569	arg1	situ					571:574	situ	571:574	situ	571:574	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	5	44	theme	component	1102:1110	arg1	variations					1112:1121	component variations	1102:1121	component variations	1102:1121	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	4	45	theme	cells	887:891	arg1	growth					893:898	Schwann cells growth	879:898	Schwann cells growth	879:898	The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated.					
29575695	0	46	with	hydrogel	80:87	arg1	bioactivity					94:104	bioactivity	94:104	bioactivity	94:104	Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth.					
29575695	1	47	from	perspective	341:351	arg1	medicine					369:376	regenerative medicine	356:376	regenerative medicine	356:376	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	2	48	theme	present	386:392	arg1	study					394:398	the present study	382:398	the present study	382:398	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	3	49	theme	degradation	762:772	arg1	behavior					774:781	in vitro degradation behavior	753:781	in vitro degradation behavior of the prepared composite hydrogel	753:816	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	9	50	theme	hydrogel	1755:1762	arg1	scaffolds					1764:1772	hydrogel scaffolds	1755:1772	hydrogel scaffolds	1755:1772	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	3	51	theme	hydrogel	809:816	arg1	morphology					673:682	morphology	673:682	morphology	673:682	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	51	theme	hydrogel	809:816	arg1	component					715:723	component	715:723	component	715:723	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	51	theme	hydrogel	809:816	arg1	porosity					685:692	porosity	685:692	porosity	685:692	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	51	theme	hydrogel	809:816	arg1	behavior					774:781	in vitro degradation behavior	753:781	in vitro degradation behavior of the prepared composite hydrogel	753:816	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	51	theme	hydrogel	809:816	arg1	properties					737:746	mechanical properties	726:746	mechanical properties	726:746	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	3	51	theme	hydrogel	809:816	arg1	behaviors					704:712	swelling behaviors	695:712	swelling behaviors	695:712	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	8	52	from	levels	1537:1542	arg1	SYR1					1602:1605	PGG0.5 SYR1	1595:1605	PGG0.5 SYR1	1595:1605	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	8	52	from	levels	1537:1542	arg1	SYR0.5					1616:1621	PGG1 SYR0.5	1611:1621	PGG1 SYR0.5	1611:1621	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	3	53	theme	prepared	790:797	arg1	hydrogel					809:816	the prepared composite hydrogel	786:816	the prepared composite hydrogel	786:816	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	4	54	theme	molecular	931:939	arg1	mechanism					941:949	the related molecular mechanism	919:949	the related molecular mechanism	919:949	The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated.					
29575695	7	55	dep	attachment	1470:1479	arg1	the					1466:1468	the	1466:1468	the	1466:1468	The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells.					
29575695	8	56	theme	myelin	1565:1570	arg1	MBP					1587:1589	MBP	1587:1589	MBP	1587:1589	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	8	56	theme	myelin	1565:1570	arg1	protein					1578:1584	myelin basic protein	1565:1584	myelin basic protein (MBP)	1565:1590	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	9	57	dep	design	1729:1734	arg1	the					1725:1727	the	1725:1727	the	1725:1727	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	9	58	theme	tissue	1784:1789	arg1	application					1803:1813	nerve tissue engineering application	1778:1813	nerve tissue engineering application	1778:1813	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	8	59	theme	protein	1578:1584	arg1	levels					1537:1542	The gene expression levels	1517:1542	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5	1517:1621	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	8	59	theme	protein	1578:1584	arg1	higher					1628:1633	higher	1628:1633	higher	1628:1633	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	3	60	theme	properties	652:661	arg1	series					626:631	A series	624:631	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel	624:816	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	7	61	theme	cells	1510:1514	arg1	proliferation					1485:1497	proliferation	1485:1497	proliferation	1485:1497	The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells.					
29575695	7	61	theme	cells	1510:1514	arg1	attachment					1470:1479	attachment	1470:1479	attachment	1470:1479	The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells.					
29575695	3	62	theme	swelling	695:702	arg1	behaviors					704:712	swelling behaviors	695:712	swelling behaviors	695:712	A series of physicochemical properties including morphology, porosity, swelling behaviors, component, mechanical properties, and in vitro degradation behavior of the prepared composite hydrogel were characterized.					
29575695	5	63	theme	PAM/GO/Gel/SA	1012:1024	arg1	hydrogels					1036:1044	the prepared PAM/GO/Gel/SA composite hydrogels	999:1044	the prepared PAM/GO/Gel/SA composite hydrogels	999:1044	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	1	64	used	used	217:220	arg2	hydrogels					150:158	Various hydrogels	142:158	Various hydrogels made from natural or synthetic polymers	142:198	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	1	65	theme	biologic	225:232	arg1	tissues					234:240	biologic tissues	225:240	biologic tissues	225:240	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	6	66	theme	surface	1128:1134	arg1	morphology					1136:1145	The surface morphology	1124:1145	The surface morphology	1124:1145	The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property.					
29575695	2	67	theme	in	568:569	arg1	polymerization					589:602	in situ free radical polymerization	568:602	in situ free radical polymerization	568:602	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	6	68	theme	degradation	1300:1310	arg1	behavior					1312:1319	no obvious degradation behavior	1289:1319	no obvious degradation behavior	1289:1319	The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property.					
29575695	0	69	theme	Schwann	120:126	arg1	cells					128:132	Schwann cells	120:132	Schwann cells growth	120:139	Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth.					
29575695	8	70	theme	PGG1	1611:1614	arg1	SYR0.5					1616:1621	PGG1 SYR0.5	1611:1621	PGG1 SYR0.5	1611:1621	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	8	71	theme	Sox10	1547:1551	arg1	levels					1537:1542	The gene expression levels	1517:1542	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5	1517:1621	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	8	71	theme	Sox10	1547:1551	arg1	higher					1628:1633	higher	1628:1633	higher	1628:1633	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	11	72	theme	Part	1866:1869	arg1	A					1871:1871	Part A	1866:1871	J Biomed Mater Res Part A: 106A: 1951-1964, 2018.	1847:1895	J Biomed Mater Res Part A: 106A: 1951-1964, 2018.					
29575695	4	73	from	effects	842:848	arg1	growth					893:898	Schwann cells growth	879:898	Schwann cells growth	879:898	The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated.					
29575695	2	74	theme	free	576:579	arg1	polymerization					589:602	in situ free radical polymerization	568:602	in situ free radical polymerization	568:602	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	6	75	theme	mechanical	1176:1185	arg1	properties					1187:1196	mechanical properties	1176:1196	mechanical properties	1176:1196	The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property.					
29575695	0	76	theme	oxide/gelatin/sodium	40:59	arg1	hydrogel					80:87	polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel	16:87	polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity	16:104	Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth.					
29575695	5	77	theme	different	1056:1064	arg1	appearance					1072:1081	different color appearance	1056:1081	different color appearance	1056:1081	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	5	77	theme	different	1056:1064	arg1	function					1090:1097	the function	1086:1097	the function of component variations	1086:1121	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	7	78	theme	composite	1427:1435	arg1	hydrogels					1437:1445	the composite hydrogels	1423:1445	the composite hydrogels	1423:1445	The culture of cells exhibited that the composite hydrogels could well support the attachment and proliferation of Schwann cells.					
29575695	0	79	theme	composite	70:78	arg1	hydrogel					80:87	polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel	16:87	polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity	16:104	Construction of polyacrylamide/graphene oxide/gelatin/sodium alginate composite hydrogel with bioactivity for promoting Schwann cells growth.					
29575695	9	80	theme	important	1676:1684	arg1	basis					1715:1719	important theoretical and experimental basis	1676:1719	important theoretical and experimental basis for the design and development of hydrogel scaffolds	1676:1772	This study may provide important theoretical and experimental basis for the design and development of hydrogel scaffolds for nerve tissue engineering application.					
29575695	8	81	theme	gene	1521:1524	arg1	levels					1537:1542	The gene expression levels	1517:1542	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5	1517:1621	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	8	81	theme	gene	1521:1524	arg1	higher					1628:1633	higher	1628:1633	higher	1628:1633	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	2	82	theme	polyacrylamide/graphene	428:450	arg1	PAM/GO/Gel/SA					483:495	PAM/GO/Gel/SA	483:495	PAM/GO/Gel/SA	483:495	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	2	82	theme	polyacrylamide/graphene	428:450	arg1	alginate					473:480	polyacrylamide/graphene oxide/gelatin/sodium alginate	428:480	polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA)	428:496	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	1	83	theme	promising	331:339	arg1	perspective					341:351	a promising perspective	329:351	a promising perspective in regenerative medicine	329:376	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
29575695	6	84	theme	concentration	1238:1250	arg1	alteration					1252:1261	the concentration alteration	1234:1261	the concentration alteration of each ingredient	1234:1280	The surface morphology, components, swelling ratio, mechanical properties, and porosity were all changed with the concentration alteration of each ingredient, while no obvious degradation behavior was observed, indicating a controllable physicochemical property.					
29575695	2	85	theme	peripheral	515:524	arg1	regeneration					532:543	peripheral nerve regeneration	515:543	peripheral nerve regeneration	515:543	In the present study, a composite hydrogel named polyacrylamide/graphene oxide/gelatin/sodium alginate (PAM/GO/Gel/SA) for accelerating peripheral nerve regeneration was fabricated through in situ free radical polymerization for the first time.					
29575695	4	86	theme	Schwann	879:885	arg1	cells					887:891	Schwann cells	879:891	Schwann cells growth	879:898	The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated.					
29575695	4	87	theme	hydrogel	867:874	arg1	effects					842:848	The effects	838:848	The effects of the composite hydrogel on Schwann cells growth	838:898	The effects of the composite hydrogel on Schwann cells growth were evaluated and the related molecular mechanism was further penetrated.					
29575695	5	88	theme	variations	1112:1121	arg1	appearance					1072:1081	different color appearance	1056:1081	different color appearance	1056:1081	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	5	88	theme	variations	1112:1121	arg1	function					1090:1097	the function	1086:1097	the function of component variations	1086:1121	The results showed that the prepared PAM/GO/Gel/SA composite hydrogels displayed different color appearance as the function of component variations.					
29575695	8	89	theme	GAP43	1554:1558	arg1	levels					1537:1542	The gene expression levels	1517:1542	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5	1517:1621	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	8	89	theme	GAP43	1554:1558	arg1	higher					1628:1633	higher	1628:1633	higher	1628:1633	The gene expression levels of Sox10, GAP43, and myelin basic protein (MBP) in PGG0.5 SYR1 and PGG1 SYR0.5 were higher than those of NC.					
29575695	1	90	theme	artificial	262:271	arg1	implants					273:280	artificial implants	262:280	artificial implants	262:280	Various hydrogels made from natural or synthetic polymers have been widely used in biologic tissues, drug delivery, and artificial implants due to their good biocompatibility, indicating a promising perspective in regenerative medicine.					
31494160	11	0	theme	sonication	1344:1353	arg1	technique					1355:1363	sonication technique	1344:1363	sonication technique with suitable particle size, entrapment efficiency and drug release	1344:1431	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	4	1	theme	sonication	521:530	arg1	technique					532:540	probe sonication technique	515:540	probe sonication technique	515:540	Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using probe sonication technique.					
31494160	5	2	theme	batch	561:565	arg1	NP-7					567:570	batch NP-7	561:570	batch NP-7	561:570	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	9	3	theme	NP-7	1107:1110	arg1	XRD					1100:1102	The XRD	1096:1102	The XRD of NP-7	1096:1110	The XRD of NP-7 displayed the existence of erlotinib in the amorphous pattern.					
31494160	11	4	dep	loaded	1281:1286	arg1	Erlotinib					1271:1279	Erlotinib	1271:1279	Erlotinib	1271:1279	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	7	5	theme	0.1 N	940:944	arg1	acid					959:962	0.1 N hydrochloric acid	940:962	0.1 N hydrochloric acid	940:962	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	1	6	theme	deaths	165:170	arg1	cause					156:160	The most common cause	140:160	The most common cause of deaths due to cancers nowadays	140:194	The most common cause of deaths due to cancers nowadays is lung cancer.					
31494160	1	6	theme	deaths	165:170	arg1	cancer					204:209	lung cancer	199:209	lung cancer	199:209	The most common cause of deaths due to cancers nowadays is lung cancer.					
31494160	7	7	theme	Phosphate	968:976	arg1	PB					986:987	PB	986:987	PB	986:987	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	7	7	theme	Phosphate	968:976	arg1	Buffer					978:983	Phosphate Buffer (PB) pH 6.8	968:995	Phosphate Buffer (PB) pH 6.8	968:995	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	0	8	theme	loaded	100:105	arg1	system					132:137	loaded chitosan nanoparticulate system	100:137	loaded chitosan nanoparticulate system	100:137	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	12	9	theme	formulated	1438:1447	arg1	nanoparticles					1449:1461	The formulated nanoparticles	1434:1461	The formulated nanoparticles	1434:1461	The formulated nanoparticles can be utilized for the treatment of lung cancer.					
31494160	7	10	theme	hydrochloric	946:957	arg1	acid					959:962	0.1 N hydrochloric acid	940:962	0.1 N hydrochloric acid	940:962	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	7	11	theme	batch	877:881	arg1	NP-7					883:886	The batch NP-7	873:886	The batch NP-7	873:886	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	3	12	theme	variables	362:370	arg1	effect					340:345	the effect	336:345	the effect of formulation variables on prepared nanoparticles using central composite design	336:427	To study the effect of formulation variables on prepared nanoparticles using central composite design.					
31494160	2	13	theme	chitosan	272:279	arg1	nanoparticles					281:293	erlotinib loaded chitosan nanoparticles	255:293	erlotinib loaded chitosan nanoparticles for their anticancer potential	255:324	The objective of this study was to prepare erlotinib loaded chitosan nanoparticles for their anticancer potential.					
31494160	11	14	theme	drug	1420:1423	arg1	release					1425:1431	drug release	1420:1431	drug release	1420:1431	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	0	15	theme	nanoparticulate	116:130	arg1	system					132:137	loaded chitosan nanoparticulate system	100:137	loaded chitosan nanoparticulate system	100:137	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	4	16	theme	gelation	493:500	arg1	method					502:507	ionic gelation method	487:507	ionic gelation method using probe sonication technique	487:540	Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using probe sonication technique.					
31494160	5	17	theme	particle	636:643	arg1	138.5 nm					651:658	138.5 nm	651:658	138.5 nm	651:658	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	5	17	theme	particle	636:643	arg1	size					645:648	a particle size	634:648	a particle size (138.5 nm) which is ideal for targeting solid tumors	634:701	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	5	17	theme	particle	636:643	arg1	ideal					670:674	ideal	670:674	ideal	670:674	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	2	18	theme	loaded	265:270	arg1	nanoparticles					281:293	erlotinib loaded chitosan nanoparticles	255:293	erlotinib loaded chitosan nanoparticles for their anticancer potential	255:324	The objective of this study was to prepare erlotinib loaded chitosan nanoparticles for their anticancer potential.					
31494160	6	19	theme	variance	716:723	arg1	Analysis					704:711	Analysis	704:711	Analysis of variance	704:723	Analysis of variance was applied to the particle size, entrapment efficiency and percent cumulative drug release to study the fitting and the significance of the model.					
31494160	0	20	theme	chitosan	107:114	arg1	system					132:137	loaded chitosan nanoparticulate system	100:137	loaded chitosan nanoparticulate system	100:137	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	4	21	theme	probe	515:519	arg1	technique					532:540	probe sonication technique	515:540	probe sonication technique	515:540	Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using probe sonication technique.					
31494160	8	22	theme	IC50	1016:1019	arg1	6.36 μM					1087:1093	6.36 μM	1087:1093	6.36 μM	1087:1093	The IC50 value of NP-7 evaluated on A549 Lung cancer cells was found to be 6.36 μM.					
31494160	8	22	theme	IC50	1016:1019	arg1	value					1021:1025	The IC50 value	1012:1025	The IC50 value of NP-7 evaluated on A549 Lung cancer cells	1012:1069	The IC50 value of NP-7 evaluated on A549 Lung cancer cells was found to be 6.36 μM.					
31494160	5	23	theme	loading	586:592	arg1	capacity					594:601	a maximum loading capacity	576:601	a maximum loading capacity	576:601	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	2	24	theme	erlotinib	255:263	arg1	nanoparticles					281:293	erlotinib loaded chitosan nanoparticles	255:293	erlotinib loaded chitosan nanoparticles for their anticancer potential	255:324	The objective of this study was to prepare erlotinib loaded chitosan nanoparticles for their anticancer potential.					
31494160	11	25	theme	particle	1379:1386	arg1	size					1388:1391	suitable particle size	1370:1391	suitable particle size	1370:1391	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	9	26	theme	erlotinib	1139:1147	arg1	existence					1126:1134	the existence	1122:1134	the existence of erlotinib in the amorphous pattern	1122:1172	The XRD of NP-7 displayed the existence of erlotinib in the amorphous pattern.					
31494160	8	27	theme	NP-7	1030:1033	arg1	6.36 μM					1087:1093	6.36 μM	1087:1093	6.36 μM	1087:1093	The IC50 value of NP-7 evaluated on A549 Lung cancer cells was found to be 6.36 μM.					
31494160	8	27	theme	NP-7	1030:1033	arg1	value					1021:1025	The IC50 value	1012:1025	The IC50 value of NP-7 evaluated on A549 Lung cancer cells	1012:1069	The IC50 value of NP-7 evaluated on A549 Lung cancer cells was found to be 6.36 μM.					
31494160	6	28	theme	cumulative	793:802	arg1	release					809:815	percent cumulative drug release	785:815	percent cumulative drug release	785:815	Analysis of variance was applied to the particle size, entrapment efficiency and percent cumulative drug release to study the fitting and the significance of the model.					
31494160	8	29	theme	cancer	1058:1063	arg1	cells					1065:1069	A549 Lung cancer cells	1048:1069	A549 Lung cancer cells	1048:1069	The IC50 value of NP-7 evaluated on A549 Lung cancer cells was found to be 6.36 μM.					
31494160	0	30	theme	Central	0:6	arg1	composite					8:16	Central composite	0:16	Central composite	0:16	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	6	31	theme	percent	785:791	arg1	release					809:815	percent cumulative drug release	785:815	percent cumulative drug release	785:815	Analysis of variance was applied to the particle size, entrapment efficiency and percent cumulative drug release to study the fitting and the significance of the model.					
31494160	2	32	theme	anticancer	305:314	arg1	potential					316:324	their anticancer potential	299:324	their anticancer potential	299:324	The objective of this study was to prepare erlotinib loaded chitosan nanoparticles for their anticancer potential.					
31494160	11	33	theme	loaded	1281:1286	arg1	nanoparticles					1297:1309	Erlotinib loaded chitosan nanoparticles	1271:1309	Erlotinib loaded chitosan nanoparticles	1271:1309	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	5	34	theme	entrapment	607:616	arg1	efficiency					618:627	entrapment efficiency	607:627	entrapment efficiency	607:627	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	1	35	dep	cancers	179:185	arg1	nowadays					187:194	nowadays	187:194	nowadays	187:194	The most common cause of deaths due to cancers nowadays is lung cancer.					
31494160	3	36	theme	prepared	375:382	arg1	nanoparticles					384:396	prepared nanoparticles	375:396	prepared nanoparticles using central composite design	375:427	To study the effect of formulation variables on prepared nanoparticles using central composite design.					
31494160	12	37	theme	cancer	1505:1510	arg1	treatment					1487:1495	the treatment	1483:1495	the treatment of lung cancer	1483:1510	The formulated nanoparticles can be utilized for the treatment of lung cancer.					
31494160	11	38	theme	entrapment	1394:1403	arg1	efficiency					1405:1414	entrapment efficiency	1394:1414	entrapment efficiency	1394:1414	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	3	39	from	effect	340:345	arg1	nanoparticles					384:396	prepared nanoparticles	375:396	prepared nanoparticles using central composite design	375:427	To study the effect of formulation variables on prepared nanoparticles using central composite design.					
31494160	10	40	theme	tablet	1251:1256	arg1	formulation					1258:1268	the marketed tablet formulation	1238:1268	the marketed tablet formulation	1238:1268	The optimized batch released erlotinib slowly in comparison to the marketed tablet formulation.					
31494160	1	41	theme	lung	199:202	arg1	cause					156:160	The most common cause	140:160	The most common cause of deaths due to cancers nowadays	140:194	The most common cause of deaths due to cancers nowadays is lung cancer.					
31494160	1	41	theme	lung	199:202	arg1	cancer					204:209	lung cancer	199:209	lung cancer	199:209	The most common cause of deaths due to cancers nowadays is lung cancer.					
31494160	8	42	theme	Lung	1053:1056	arg1	cells					1065:1069	A549 Lung cancer cells	1048:1069	A549 Lung cancer cells	1048:1069	The IC50 value of NP-7 evaluated on A549 Lung cancer cells was found to be 6.36 μM.					
31494160	6	43	theme	model	866:870	arg1	fitting					830:836	the fitting	826:836	the fitting	826:836	Analysis of variance was applied to the particle size, entrapment efficiency and percent cumulative drug release to study the fitting and the significance of the model.					
31494160	6	43	theme	model	866:870	arg1	significance					846:857	the significance	842:857	the significance of the model	842:870	Analysis of variance was applied to the particle size, entrapment efficiency and percent cumulative drug release to study the fitting and the significance of the model.					
31494160	0	44	dep	in	61:62	arg1	vitro					64:68	vitro	64:68	vitro	64:68	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	10	45	theme	marketed	1242:1249	arg1	formulation					1258:1268	the marketed tablet formulation	1238:1268	the marketed tablet formulation	1238:1268	The optimized batch released erlotinib slowly in comparison to the marketed tablet formulation.					
31494160	9	46	from	existence	1126:1134	arg1	pattern					1166:1172	the amorphous pattern	1152:1172	the amorphous pattern	1152:1172	The XRD of NP-7 displayed the existence of erlotinib in the amorphous pattern.					
31494160	12	47	used	utilized	1470:1477	arg2	nanoparticles					1449:1461	The formulated nanoparticles	1434:1461	The formulated nanoparticles	1434:1461	The formulated nanoparticles can be utilized for the treatment of lung cancer.					
31494160	8	48	theme	A549	1048:1051	arg1	cells					1065:1069	A549 Lung cancer cells	1048:1069	A549 Lung cancer cells	1048:1069	The IC50 value of NP-7 evaluated on A549 Lung cancer cells was found to be 6.36 μM.					
31494160	4	49	theme	ionic	487:491	arg1	method					502:507	ionic gelation method	487:507	ionic gelation method using probe sonication technique	487:540	Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using probe sonication technique.					
31494160	3	50	theme	central	404:410	arg1	design					422:427	central composite design	404:427	central composite design	404:427	To study the effect of formulation variables on prepared nanoparticles using central composite design.					
31494160	3	51	theme	formulation	350:360	arg1	variables					362:370	formulation variables	350:370	formulation variables	350:370	To study the effect of formulation variables on prepared nanoparticles using central composite design.					
31494160	11	52	theme	suitable	1370:1377	arg1	size					1388:1391	suitable particle size	1370:1391	suitable particle size	1370:1391	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	6	53	theme	entrapment	759:768	arg1	efficiency					770:779	entrapment efficiency	759:779	entrapment efficiency	759:779	Analysis of variance was applied to the particle size, entrapment efficiency and percent cumulative drug release to study the fitting and the significance of the model.					
31494160	3	54	theme	composite	412:420	arg1	design					422:427	central composite design	404:427	central composite design	404:427	To study the effect of formulation variables on prepared nanoparticles using central composite design.					
31494160	5	55	theme	solid	690:694	arg1	tumors					696:701	solid tumors	690:701	solid tumors	690:701	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	4	56	theme	loaded	440:445	arg1	nanoparticles					456:468	Erlotinib loaded chitosan nanoparticles	430:468	Erlotinib loaded chitosan nanoparticles	430:468	Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using probe sonication technique.					
31494160	12	57	theme	lung	1500:1503	arg1	cancer					1505:1510	lung cancer	1500:1510	lung cancer	1500:1510	The formulated nanoparticles can be utilized for the treatment of lung cancer.					
31494160	10	58	theme	optimized	1179:1187	arg1	batch					1189:1193	The optimized batch	1175:1193	The optimized batch	1175:1193	The optimized batch released erlotinib slowly in comparison to the marketed tablet formulation.					
31494160	7	59	theme	drug	913:916	arg1	release					918:924	drug release	913:924	drug release	913:924	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	6	60	theme	drug	804:807	arg1	release					809:815	percent cumulative drug release	785:815	percent cumulative drug release	785:815	Analysis of variance was applied to the particle size, entrapment efficiency and percent cumulative drug release to study the fitting and the significance of the model.					
31494160	0	61	dep	formulation	27:37	arg1	system					132:137	loaded chitosan nanoparticulate system	100:137	loaded chitosan nanoparticulate system	100:137	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	5	62	theme	maximum	578:584	arg1	capacity					594:601	a maximum loading capacity	576:601	a maximum loading capacity	576:601	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	5	63	contain	has	572:574	arg2	capacity					594:601	a maximum loading capacity	576:601	a maximum loading capacity	576:601	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	5	63	contain	has	572:574	arg1	NP-7					567:570	batch NP-7	561:570	batch NP-7	561:570	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	5	63	contain	has	572:574	arg2	efficiency					618:627	entrapment efficiency	607:627	entrapment efficiency	607:627	It was found that batch NP-7 has a maximum loading capacity and entrapment efficiency with a particle size (138.5 nm) which is ideal for targeting solid tumors.					
31494160	11	64	theme	chitosan	1288:1295	arg1	nanoparticles					1297:1309	Erlotinib loaded chitosan nanoparticles	1271:1309	Erlotinib loaded chitosan nanoparticles	1271:1309	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	7	65	dep	%	900:900	arg1	release					918:924	drug release	913:924	drug release	913:924	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	11	66	with	technique	1355:1363	arg1	size					1388:1391	suitable particle size	1370:1391	suitable particle size	1370:1391	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	11	66	with	technique	1355:1363	arg1	efficiency					1405:1414	entrapment efficiency	1394:1414	entrapment efficiency	1394:1414	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	11	66	with	technique	1355:1363	arg1	release					1425:1431	drug release	1420:1431	drug release	1420:1431	Erlotinib loaded chitosan nanoparticles were prepared successfully using sonication technique with suitable particle size, entrapment efficiency and drug release.					
31494160	6	67	theme	particle	744:751	arg1	size					753:756	the particle size	740:756	the particle size	740:756	Analysis of variance was applied to the particle size, entrapment efficiency and percent cumulative drug release to study the fitting and the significance of the model.					
31494160	0	68	theme	cytotoxic	70:78	arg1	effect					80:85	in vitro cytotoxic effect	61:85	in vitro cytotoxic effect of erlotinib	61:98	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	4	69	theme	chitosan	447:454	arg1	nanoparticles					456:468	Erlotinib loaded chitosan nanoparticles	430:468	Erlotinib loaded chitosan nanoparticles	430:468	Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using probe sonication technique.					
31494160	1	70	theme	common	149:154	arg1	cause					156:160	The most common cause	140:160	The most common cause of deaths due to cancers nowadays	140:194	The most common cause of deaths due to cancers nowadays is lung cancer.					
31494160	1	70	theme	common	149:154	arg1	cancer					204:209	lung cancer	199:209	lung cancer	199:209	The most common cause of deaths due to cancers nowadays is lung cancer.					
31494160	9	71	theme	amorphous	1156:1164	arg1	pattern					1166:1172	the amorphous pattern	1152:1172	the amorphous pattern	1152:1172	The XRD of NP-7 displayed the existence of erlotinib in the amorphous pattern.					
31494160	4	72	dep	loaded	440:445	arg1	Erlotinib					430:438	Erlotinib	430:438	Erlotinib	430:438	Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using probe sonication technique.					
31494160	0	73	theme	in	61:62	arg1	effect					80:85	in vitro cytotoxic effect	61:85	in vitro cytotoxic effect of erlotinib	61:98	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	2	74	theme	study	234:238	arg1	objective					216:224	The objective	212:224	The objective of this study	212:238	The objective of this study was to prepare erlotinib loaded chitosan nanoparticles for their anticancer potential.					
31494160	7	75	from	24 h	932:935	arg1	PB					986:987	PB	986:987	PB	986:987	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	7	75	from	24 h	932:935	arg1	acid					959:962	0.1 N hydrochloric acid	940:962	0.1 N hydrochloric acid	940:962	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	7	75	from	24 h	932:935	arg1	Buffer					978:983	Phosphate Buffer (PB) pH 6.8	968:995	Phosphate Buffer (PB) pH 6.8	968:995	The batch NP-7 showed 91.57% and 39.78% drug release after 24 h in 0.1 N hydrochloric acid and Phosphate Buffer (PB) pH 6.8, respectively.					
31494160	0	76	theme	erlotinib	90:98	arg1	effect					80:85	in vitro cytotoxic effect	61:85	in vitro cytotoxic effect of erlotinib	61:98	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	0	76	theme	erlotinib	90:98	arg1	characterization					40:55	characterization	40:55	characterization	40:55	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
31494160	0	76	theme	erlotinib	90:98	arg1	formulation					27:37	formulation	27:37	formulation	27:37	Central composite designed formulation, characterization and in vitro cytotoxic effect of erlotinib loaded chitosan nanoparticulate system.					
30922916	1	0	theme	worldwide	261:269	arg1	focus					242:246	the focus	238:246	the focus of attention worldwide	238:269	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	3	1	theme	dye	713:715	arg1	removal					675:681	efficient removal	665:681	efficient removal (~83%) of methylene blue (MB) dye	665:715	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	3	1	theme	dye	713:715	arg1	%					687:687	~83%	684:687	~83%	684:687	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	9	2	theme	supplies	1791:1798	arg1	decontamination					1766:1780	the decontamination	1762:1780	the decontamination of water supplies	1762:1798	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	6	3	theme	lignin	1229:1234	arg1	composite					1236:1244	chitosan/bulk lignin composite	1215:1244	chitosan/bulk lignin composite	1215:1244	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	1	4	theme	nano-lignin	175:185	arg1	utilization					120:130	The utilization	116:130	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite	116:225	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	3	5	theme	chitosan/nano-lignin	577:596	arg1	material					608:615	a new chitosan/nano-lignin composite material	571:615	a new chitosan/nano-lignin composite material	571:615	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	6	6	theme	native	1195:1200	arg1	chitosan					1202:1209	chitosan	1202:1209	chitosan	1202:1209	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	3	7	theme	natural	723:729	arg1	conditions					734:743	natural pH conditions	723:743	natural pH conditions	723:743	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	1	8	theme	fabricating	205:215	arg1	composite					217:225	fabricating composite	205:225	fabricating composite	205:225	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	1	9	from	composite	217:225	arg1	bio-additve					190:200	bio-additve	190:200	bio-additve	190:200	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	8	10	theme	real	1579:1582	arg1	samples					1590:1596	spiked real water samples	1572:1596	spiked real water samples	1572:1596	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	4	11	with	adsorption	892:901	arg1	capacity					929:936	a maximum adsorption capacity	908:936	a maximum adsorption capacity of 74.07 mg g-1	908:952	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	4	12	theme	adsorption	918:927	arg1	capacity					929:936	a maximum adsorption capacity	908:936	a maximum adsorption capacity of 74.07 mg g-1	908:952	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	0	13	theme	enhanced	58:65	arg1	removal					67:73	enhanced removal	58:73	enhanced removal of dye pollution from aqueous solutions	58:113	Chitosan/nano-lignin based composite as a new sorbent for enhanced removal of dye pollution from aqueous solutions.					
30922916	2	14	theme	acidified	486:494	arg1	system					504:509	an acidified aqueous system	483:509	an acidified aqueous system	483:509	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	1	15	theme	group	160:164	arg1	nano-lignin					175:185	renewable and functional group enriched nano-lignin	135:185	renewable and functional group enriched nano-lignin	135:185	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	2	16	theme	modification	532:543	arg1	steps					545:549	any chemical modification steps	519:549	any chemical modification steps	519:549	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	4	17	theme	adsorption	750:759	arg1	process					761:767	The adsorption process	746:767	The adsorption process	746:767	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	0	18	theme	pollution	82:90	arg1	removal					67:73	enhanced removal	58:73	enhanced removal of dye pollution from aqueous solutions	58:113	Chitosan/nano-lignin based composite as a new sorbent for enhanced removal of dye pollution from aqueous solutions.					
30922916	8	19	theme	repeated	1443:1450	arg1	cycles					1476:1481	four repeated adsorption/regeneration cycles	1438:1481	four repeated adsorption/regeneration cycles	1438:1481	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	0	20	theme	aqueous	97:103	arg1	solutions					105:113	aqueous solutions	97:113	aqueous solutions	97:113	Chitosan/nano-lignin based composite as a new sorbent for enhanced removal of dye pollution from aqueous solutions.					
30922916	6	21	theme	composite	1107:1115	arg1	chitosan					1098:1105	chitosan composite	1098:1115	chitosan composite with nano-lignin	1098:1132	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	2	22	from	tetrahydrofuran	464:478	arg1	system					504:509	an acidified aqueous system	483:509	an acidified aqueous system	483:509	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	2	23	theme	kernel	433:438	arg1	shell					440:444	palm kernel shell	428:444	palm kernel shell	428:444	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	2	23	theme	kernel	433:438	arg1	waste					421:425	agro-industrial waste	405:425	agro-industrial waste (palm kernel shell)	405:445	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	8	24	theme	recyclable	1492:1501	arg1	nature					1503:1508	recyclable nature	1492:1508	recyclable nature of as-prepared composite	1492:1533	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	3	25	dep	blue	703:706	arg1	MB					709:710	MB	709:710	MB	709:710	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	6	26	with	chitosan	1098:1105	arg1	nano-lignin					1122:1132	nano-lignin	1122:1132	nano-lignin	1122:1132	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	7	27	theme	lignin	1383:1388	arg1	nanoparticles					1390:1402	lignin nanoparticles	1383:1402	lignin nanoparticles	1383:1402	This could essentially be attributed to synergistic effects of size particularity (nano-effect) and incorporated functionalities due to lignin nanoparticles.					
30922916	6	28	dep	showed	1134:1139	arg1	compared					1183:1190	compared	1183:1190	showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite	1134:1244	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	2	29	theme	agro-industrial	405:419	arg1	shell					440:444	palm kernel shell	428:444	palm kernel shell	428:444	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	2	29	theme	agro-industrial	405:419	arg1	waste					421:425	agro-industrial waste	405:425	agro-industrial waste (palm kernel shell)	405:445	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	2	30	theme	recyclable	453:462	arg1	tetrahydrofuran					464:478	recyclable tetrahydrofuran	453:478	recyclable tetrahydrofuran in an acidified aqueous system	453:509	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	4	31	theme	adsorption	833:842	arg1	data					844:847	the adsorption data	829:847	the adsorption data using Langmuir model	829:868	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	5	32	theme	process	1059:1065	arg1	nature					1036:1041	spontaneous and endothermic nature	1008:1041	spontaneous and endothermic nature of the sorption process	1008:1065	Moreover, thermodynamic study of the system revealed spontaneous and endothermic nature of the sorption process.					
30922916	9	33	theme	water	1785:1789	arg1	supplies					1791:1798	water supplies	1785:1798	water supplies	1785:1798	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	9	34	theme	adsorptive	1738:1747	arg1	bio-nanocomposite					1668:1684	as-prepared bio-nanocomposite	1656:1684	as-prepared bio-nanocomposite	1656:1684	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	9	34	theme	adsorptive	1738:1747	arg1	platform					1749:1756	an efficient and reusable adsorptive platform	1712:1756	an efficient and reusable adsorptive platform for the decontamination of water supplies	1712:1798	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	4	35	theme	adequate	809:816	arg1	fitting					818:824	adequate fitting	809:824	adequate fitting of the adsorption data using Langmuir model	809:868	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	6	36	theme	better	1141:1146	arg1	performance					1148:1158	better performance	1141:1158	better performance in dye decontamination	1141:1181	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	1	37	theme	attention	251:259	arg1	worldwide					261:269	attention worldwide	251:269	attention worldwide	251:269	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	7	38	theme	size	1310:1313	arg1	particularity					1315:1327	size particularity	1310:1327	size particularity (nano-effect)	1310:1341	This could essentially be attributed to synergistic effects of size particularity (nano-effect) and incorporated functionalities due to lignin nanoparticles.					
30922916	7	38	theme	size	1310:1313	arg1	nano-effect					1330:1340	nano-effect	1330:1340	nano-effect	1330:1340	This could essentially be attributed to synergistic effects of size particularity (nano-effect) and incorporated functionalities due to lignin nanoparticles.					
30922916	8	39	theme	composite	1525:1533	arg1	nature					1503:1508	recyclable nature	1492:1508	recyclable nature of as-prepared composite	1492:1533	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	3	40	theme	new	573:575	arg1	material					608:615	a new chitosan/nano-lignin composite material	571:615	a new chitosan/nano-lignin composite material	571:615	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	4	41	theme	monolayer	882:890	arg1	adsorption					892:901	a monolayer adsorption	880:901	a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1	880:952	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	2	42	theme	lignin	280:285	arg1	nanoparticles					287:299	lignin nanoparticles	280:299	lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm	280:375	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	1	43	theme	enriched	166:173	arg1	nano-lignin					175:185	renewable and functional group enriched nano-lignin	135:185	renewable and functional group enriched nano-lignin	135:185	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	4	44	theme	data	844:847	arg1	fitting					818:824	adequate fitting	809:824	adequate fitting of the adsorption data using Langmuir model	809:868	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	6	45	theme	chitosan/bulk	1215:1227	arg1	composite					1236:1244	chitosan/bulk lignin composite	1215:1244	chitosan/bulk lignin composite	1215:1244	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	2	46	theme	hollow	316:321	arg1	spheres					323:329	hollow spheres	316:329	hollow spheres with the diameter of the order of 138 ± 39 nm	316:375	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	4	47	theme	Langmuir	855:862	arg1	model					864:868	Langmuir model	855:868	Langmuir model	855:868	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	8	48	theme	spiked	1572:1577	arg1	samples					1590:1596	spiked real water samples	1572:1596	spiked real water samples	1572:1596	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	3	49	theme	pH	731:732	arg1	conditions					734:743	natural pH conditions	723:743	natural pH conditions	723:743	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	4	50	theme	74.07 mg g-1	941:952	arg1	capacity					929:936	a maximum adsorption capacity	908:936	a maximum adsorption capacity of 74.07 mg g-1	908:952	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	8	51	theme	water	1584:1588	arg1	samples					1590:1596	spiked real water samples	1572:1596	spiked real water samples	1572:1596	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	3	52	theme	composite	598:606	arg1	material					608:615	a new chitosan/nano-lignin composite material	571:615	a new chitosan/nano-lignin composite material	571:615	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	0	53	from	solutions	105:113	arg1	removal					67:73	enhanced removal	58:73	enhanced removal of dye pollution from aqueous solutions	58:113	Chitosan/nano-lignin based composite as a new sorbent for enhanced removal of dye pollution from aqueous solutions.					
30922916	5	54	theme	spontaneous	1008:1018	arg1	nature					1036:1041	spontaneous and endothermic nature	1008:1041	spontaneous and endothermic nature of the sorption process	1008:1065	Moreover, thermodynamic study of the system revealed spontaneous and endothermic nature of the sorption process.					
30922916	4	55	theme	maximum	910:916	arg1	capacity					929:936	a maximum adsorption capacity	908:936	a maximum adsorption capacity of 74.07 mg g-1	908:952	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	7	56	theme	synergistic	1287:1297	arg1	effects					1299:1305	synergistic effects	1287:1305	synergistic effects of size particularity (nano-effect)	1287:1341	This could essentially be attributed to synergistic effects of size particularity (nano-effect) and incorporated functionalities due to lignin nanoparticles.					
30922916	3	57	theme	efficient	627:635	arg1	sorbent					637:643	highly efficient sorbent	620:643	highly efficient sorbent	620:643	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	1	58	from	bio-additve	190:200	arg1	composite					217:225	fabricating composite	205:225	fabricating composite	205:225	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	1	59	theme	renewable	135:143	arg1	nano-lignin					175:185	renewable and functional group enriched nano-lignin	135:185	renewable and functional group enriched nano-lignin	135:185	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	2	60	from	nanoparticles	287:299	arg1	form					308:311	the form	304:311	the form of hollow spheres with the diameter of the order of 138 ± 39 nm	304:375	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	2	61	theme	aqueous	496:502	arg1	system					504:509	an acidified aqueous system	483:509	an acidified aqueous system	483:509	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	0	62	theme	dye	78:80	arg1	pollution					82:90	dye pollution	78:90	dye pollution	78:90	Chitosan/nano-lignin based composite as a new sorbent for enhanced removal of dye pollution from aqueous solutions.					
30922916	1	63	theme	functional	149:158	arg1	nano-lignin					175:185	renewable and functional group enriched nano-lignin	135:185	renewable and functional group enriched nano-lignin	135:185	The utilization of renewable and functional group enriched nano-lignin as bio-additve in fabricating composite has become the focus of attention worldwide.					
30922916	8	64	theme	Recyclability	1405:1417	arg1	study					1419:1423	Recyclability study	1405:1423	Recyclability study performed in four repeated adsorption/regeneration cycles	1405:1481	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	2	65	theme	chemical	523:530	arg1	steps					545:549	any chemical modification steps	519:549	any chemical modification steps	519:549	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	5	66	theme	system	992:997	arg1	study					979:983	thermodynamic study	965:983	thermodynamic study of the system	965:997	Moreover, thermodynamic study of the system revealed spontaneous and endothermic nature of the sorption process.					
30922916	8	67	theme	adsorption/regeneration	1452:1474	arg1	cycles					1476:1481	four repeated adsorption/regeneration cycles	1438:1481	four repeated adsorption/regeneration cycles	1438:1481	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	2	68	theme	138 ± 39 nm	365:375	arg1	order					356:360	the order	352:360	the order of 138 ± 39 nm	352:375	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	5	69	theme	thermodynamic	965:977	arg1	study					979:983	thermodynamic study	965:983	thermodynamic study of the system	965:997	Moreover, thermodynamic study of the system revealed spontaneous and endothermic nature of the sorption process.					
30922916	2	70	theme	palm	428:431	arg1	shell					440:444	palm kernel shell	428:444	palm kernel shell	428:444	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	2	70	theme	palm	428:431	arg1	waste					421:425	agro-industrial waste	405:425	agro-industrial waste (palm kernel shell)	405:445	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	9	71	theme	as-prepared	1656:1666	arg1	bio-nanocomposite					1668:1684	as-prepared bio-nanocomposite	1656:1684	as-prepared bio-nanocomposite	1656:1684	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	9	71	theme	as-prepared	1656:1666	arg1	platform					1749:1756	an efficient and reusable adsorptive platform	1712:1756	an efficient and reusable adsorptive platform for the decontamination of water supplies	1712:1798	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	3	72	theme	efficient	665:673	arg1	removal					675:681	efficient removal	665:681	efficient removal (~83%) of methylene blue (MB) dye	665:715	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	3	72	theme	efficient	665:673	arg1	%					687:687	~83%	684:687	~83%	684:687	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	0	73	theme	new	42:44	arg1	composite					27:35	composite	27:35	composite	27:35	Chitosan/nano-lignin based composite as a new sorbent for enhanced removal of dye pollution from aqueous solutions.					
30922916	0	73	theme	new	42:44	arg1	sorbent					46:52	a new sorbent	40:52	a new sorbent for enhanced removal of dye pollution from aqueous solutions	40:113	Chitosan/nano-lignin based composite as a new sorbent for enhanced removal of dye pollution from aqueous solutions.					
30922916	2	74	with	spheres	323:329	arg1	diameter					340:347	the diameter	336:347	the diameter of the order of 138 ± 39 nm	336:375	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	6	75	theme	Further	1068:1074	arg1	studies					1076:1082	Further studies	1068:1082	Further studies	1068:1082	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	5	76	theme	sorption	1050:1057	arg1	process					1059:1065	the sorption process	1046:1065	the sorption process	1046:1065	Moreover, thermodynamic study of the system revealed spontaneous and endothermic nature of the sorption process.					
30922916	8	77	theme	as-prepared	1513:1523	arg1	composite					1525:1533	as-prepared composite	1513:1533	as-prepared composite	1513:1533	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	9	78	theme	efficient	1715:1723	arg1	bio-nanocomposite					1668:1684	as-prepared bio-nanocomposite	1656:1684	as-prepared bio-nanocomposite	1656:1684	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	9	78	theme	efficient	1715:1723	arg1	platform					1749:1756	an efficient and reusable adsorptive platform	1712:1756	an efficient and reusable adsorptive platform for the decontamination of water supplies	1712:1798	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	9	79	theme	reusable	1729:1736	arg1	bio-nanocomposite					1668:1684	as-prepared bio-nanocomposite	1656:1684	as-prepared bio-nanocomposite	1656:1684	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	9	79	theme	reusable	1729:1736	arg1	platform					1749:1756	an efficient and reusable adsorptive platform	1712:1756	an efficient and reusable adsorptive platform for the decontamination of water supplies	1712:1798	Based on this study, as-prepared bio-nanocomposite may thus be considered as an efficient and reusable adsorptive platform for the decontamination of water supplies.					
30922916	6	80	theme	dye	1163:1165	arg1	decontamination					1167:1181	dye decontamination	1163:1181	dye decontamination	1163:1181	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	4	81	theme	pseudo-second-order	776:794	arg1	kinetics					796:803	pseudo-second-order kinetics	776:803	pseudo-second-order kinetics	776:803	The adsorption process obeyed pseudo-second-order kinetics and adequate fitting of the adsorption data using Langmuir model suggested a monolayer adsorption with a maximum adsorption capacity of 74.07 mg g-1.					
30922916	2	82	theme	spheres	323:329	arg1	form					308:311	the form	304:311	the form of hollow spheres with the diameter of the order of 138 ± 39 nm	304:375	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
30922916	5	83	theme	endothermic	1024:1034	arg1	nature					1036:1041	spontaneous and endothermic nature	1008:1041	spontaneous and endothermic nature of the sorption process	1008:1065	Moreover, thermodynamic study of the system revealed spontaneous and endothermic nature of the sorption process.					
30922916	7	84	theme	particularity	1315:1327	arg1	effects					1299:1305	synergistic effects	1287:1305	synergistic effects of size particularity (nano-effect)	1287:1341	This could essentially be attributed to synergistic effects of size particularity (nano-effect) and incorporated functionalities due to lignin nanoparticles.					
30922916	3	85	theme	blue	703:706	arg1	dye					713:715	methylene blue (MB) dye	693:715	methylene blue (MB) dye	693:715	We then fabricated a new chitosan/nano-lignin composite material as highly efficient sorbent, as demonstrated by efficient removal (~83%) of methylene blue (MB) dye under natural pH conditions.					
30922916	8	86	theme	adsorption	1543:1552	arg1	experiments					1554:1564	adsorption experiments	1543:1564	adsorption experiments using spiked real water samples	1543:1596	Recyclability study performed in four repeated adsorption/regeneration cycles revealed recyclable nature of as-prepared composite, whilst adsorption experiments using spiked real water samples indicated recoveries as high as 89%.					
30922916	6	87	from	performance	1148:1158	arg1	decontamination					1167:1181	dye decontamination	1163:1181	dye decontamination	1163:1181	Further studies revealed that chitosan composite with nano-lignin showed better performance in dye decontamination compared to native chitosan and chitosan/bulk lignin composite.					
30922916	2	88	theme	order	356:360	arg1	diameter					340:347	the diameter	336:347	the diameter of the order of 138 ± 39 nm	336:375	Herein, lignin nanoparticles in the form of hollow spheres with the diameter of the order of 138 ± 39 nm were directly prepared from agro-industrial waste (palm kernel shell) using recyclable tetrahydrofuran in an acidified aqueous system without any chemical modification steps.					
29649233	7	0	theme	paramylon	1182:1190	arg1	paramylon					1182:1190	paramylon	1182:1190	paramylon	1182:1190	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	7	0	theme	paramylon	1182:1190	arg1	amounts					1171:1177	large amounts	1165:1177	large amounts of paramylon	1165:1190	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	10	1	theme	phototrophic	1596:1607	arg1	cells					1609:1613	phototrophic cells	1596:1613	phototrophic cells	1596:1613	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	13	2	theme	mg	1986:1987	arg1	biomass					1993:1999	1 to 5 mg g-1 biomass	1979:1999	1 to 5 mg g-1 biomass	1979:1999	Eicosapentaenoic and docosahexaenoic acids were detected at 1 to 5 mg g-1 biomass.					
29649233	4	3	theme	heterotrophic	760:772	arg1	conditions					789:798	either heterotrophic or mixotrophic conditions	753:798	either heterotrophic or mixotrophic conditions	753:798	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	4	4	theme	mixotrophic	777:787	arg1	conditions					789:798	either heterotrophic or mixotrophic conditions	753:798	either heterotrophic or mixotrophic conditions	753:798	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	1	5	theme	fatty	189:193	arg1	acids					195:199	unsaturated fatty acids	177:199	unsaturated fatty acids	177:199	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	12	6	theme	light	1856:1860	arg1	presence					1844:1851	the presence	1840:1851	the presence of light	1840:1860	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	5	7	from	cultures	955:962	arg1	rate					921:924	the rate	917:924	the rate of exponential growth in all cultures	917:962	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	4	8	theme	growth	875:880	arg1	longer					886:891	longer	886:891	longer	886:891	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	4	8	theme	growth	875:880	arg1	duration					851:858	the duration	847:858	the duration of exponential growth	847:880	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	10	9	theme	acid	1510:1513	arg1	similar					1568:1574	similar	1568:1574	similar	1568:1574	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	10	9	theme	acid	1510:1513	arg1	lower					1624:1628	lower	1624:1628	lower	1624:1628	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	10	9	theme	acid	1510:1513	arg1	content					1515:1521	The fatty acid content	1500:1521	The fatty acid content of cells grown in mixotrophic conditions	1500:1562	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	7	10	contain	contain	1263:1269	arg2	paramylon					1281:1289	up to 50% paramylon	1271:1289	up to 50% paramylon	1271:1289	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	7	10	contain	contain	1263:1269	arg1	cells					1251:1255	phototrophic cells	1238:1255	phototrophic cells	1238:1255	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	11	11	theme	fatty	1711:1715	arg1	acids					1717:1721	less unsaturated fatty acids	1694:1721	less unsaturated fatty acids	1694:1721	Heterotrophic cells contained less unsaturated fatty acids than phototrophic or mixotrophic cells.					
29649233	13	12	theme	docosahexaenoic	1940:1954	arg1	acids					1956:1960	Eicosapentaenoic and docosahexaenoic acids	1919:1960	Eicosapentaenoic and docosahexaenoic acids	1919:1960	Eicosapentaenoic and docosahexaenoic acids were detected at 1 to 5 mg g-1 biomass.					
29649233	8	13	dep	%	1348:1348	arg1	20					1346:1347	20	1346:1347	20	1346:1347	These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected.					
29649233	9	14	dep	acids	1422:1426	arg1	%					1414:1414	%	1414:1414	%	1414:1414	The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated.					
29649233	9	14	dep	acids	1422:1426	arg1	measured					1429:1436	measured	1429:1436	measured as fatty acid methyl esters	1429:1464	The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated.					
29649233	5	15	theme	growth	984:989	arg1	rate					991:994	the linear growth rate	973:994	the linear growth rate	973:994	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	9	16	theme	fatty	1416:1420	arg1	acids					1422:1426	about 8% fatty acids	1407:1426	about 8% fatty acids (measured as fatty acid methyl esters)	1407:1465	The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated.					
29649233	2	17	theme	cell	470:473	arg1	composition					475:485	cell composition	470:485	cell composition	470:485	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	0	18	theme	temperature	61:71	arg1	conditions					92:101	different temperature, light and trophic conditions	51:101	different temperature, light and trophic conditions	51:101	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	8	19	contain	contained	1304:1312	arg2	compounds					1361:1369	less than 20% lipophilic compounds	1336:1369	less than 20% lipophilic compounds	1336:1369	These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected.					
29649233	8	19	contain	contained	1304:1312	arg1	cells					1298:1302	These cells	1292:1302	These cells	1292:1302	These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected.					
29649233	8	19	contain	contained	1304:1312	arg2	protein					1324:1330	up to 33% protein	1314:1330	up to 33% protein	1314:1330	These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected.					
29649233	0	20	theme	light	74:78	arg1	conditions					92:101	different temperature, light and trophic conditions	51:101	different temperature, light and trophic conditions	51:101	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	12	21	theme	mg	1803:1804	arg1	g-1					1806:1808	5 to 18 mg g-1	1795:1808	5 to 18 mg g-1 dry biomass in cells grown in the presence of light	1795:1860	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	8	22	dep	%	1322:1322	arg1	33					1320:1321	33	1320:1321	33	1320:1321	These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected.					
29649233	9	23	dep	%	1414:1414	arg1	8					1413:1413	8	1413:1413	8	1413:1413	The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated.					
29649233	0	24	dep	temperature	61:71	arg1	different					51:59	different	51:59	different	51:59	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	0	25	theme	trophic	84:90	arg1	conditions					92:101	different temperature, light and trophic conditions	51:101	different temperature, light and trophic conditions	51:101	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	5	26	theme	phototrophic	1027:1038	arg1	conditions					1040:1049	phototrophic conditions	1027:1049	phototrophic conditions	1027:1049	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	12	27	theme	dry	1810:1812	arg1	biomass					1814:1820	5 to 18 mg g-1 dry biomass	1795:1820	5 to 18 mg g-1 dry biomass in cells grown in the presence of light	1795:1860	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	9	28	theme	acid	1447:1450	arg1	esters					1459:1464	fatty acid methyl esters	1441:1464	fatty acid methyl esters	1441:1464	The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated.					
29649233	9	29	contain	contained	1397:1405	arg1	biomass					1389:1395	The biomass	1385:1395	The biomass	1385:1395	The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated.					
29649233	9	29	contain	contained	1397:1405	arg2	acids					1422:1426	about 8% fatty acids	1407:1426	about 8% fatty acids (measured as fatty acid methyl esters)	1407:1465	The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated.					
29649233	2	30	theme	mixo-	414:418	arg1	conditions					438:447	photo-, mixo- and heterotrophic conditions	406:447	photo-, mixo- and heterotrophic conditions	406:447	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	2	30	theme	mixo-	414:418	arg1	carbon					398:403	organic carbon	390:403	organic carbon (photo-, mixo- and heterotrophic conditions)	390:448	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	4	31	theme	GROWTH	663:668	arg1	rates					686:690	CELL GROWTH Specific growth rates	658:690	CELL GROWTH Specific growth rates	658:690	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	10	32	theme	cells	1526:1530	arg1	similar					1568:1574	similar	1568:1574	similar	1568:1574	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	10	32	theme	cells	1526:1530	arg1	lower					1624:1628	lower	1624:1628	lower	1624:1628	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	10	32	theme	cells	1526:1530	arg1	content					1515:1521	The fatty acid content	1500:1521	The fatty acid content of cells grown in mixotrophic conditions	1500:1562	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	0	33	theme	Euglena	0:6	arg1	growth					17:22	growth	17:22	growth	17:22	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	0	33	theme	Euglena	0:6	arg1	composition					33:43	cell composition	28:43	cell composition	28:43	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	0	33	theme	Euglena	0:6	arg1	gracilis					8:15	Euglena gracilis growth and cell composition	0:43	Euglena gracilis growth and cell composition	0:43	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	2	34	theme	photo-	406:411	arg1	conditions					438:447	photo-, mixo- and heterotrophic conditions	406:447	photo-, mixo- and heterotrophic conditions	406:447	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	2	34	theme	photo-	406:411	arg1	carbon					398:403	organic carbon	390:403	organic carbon (photo-, mixo- and heterotrophic conditions)	390:448	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	4	35	theme	growth	679:684	arg1	rates					686:690	CELL GROWTH Specific growth rates	658:690	CELL GROWTH Specific growth rates	658:690	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	1	36	theme	other	269:273	arg1	compounds					284:292	other valuable compounds	269:292	other valuable compounds	269:292	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	12	37	theme	α-Linolenic	1763:1773	arg1	acid					1775:1778	α-Linolenic acid	1763:1778	α-Linolenic acid	1763:1778	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	1	38	theme	BACKGROUND	104:113	arg1	protist					150:156	a photosynthetic protist	133:156	a photosynthetic protist	133:156	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	1	38	theme	BACKGROUND	104:113	arg1	gracilis					123:130	BACKGROUND Euglena gracilis	104:130	BACKGROUND Euglena gracilis	104:130	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	14	39	theme	phytol	2063:2068	arg1	production					2035:2044	the production	2031:2044	the production of vitamin E and phytol	2031:2068	Light was also important for the production of vitamin E and phytol.					
29649233	7	40	theme	CELL	1101:1104	arg1	COMPOSITION					1106:1116	CELL COMPOSITION	1101:1116	CELL COMPOSITION Although E. gracilis	1101:1137	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	0	41	theme	cell	28:31	arg1	composition					33:43	cell composition	28:43	cell composition	28:43	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	0	41	theme	cell	28:31	arg1	gracilis					8:15	Euglena gracilis growth and cell composition	0:43	Euglena gracilis growth and cell composition	0:43	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	2	42	theme	heterotrophic	424:436	arg1	conditions					438:447	photo-, mixo- and heterotrophic conditions	406:447	photo-, mixo- and heterotrophic conditions	406:447	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	2	42	theme	heterotrophic	424:436	arg1	carbon					398:403	organic carbon	390:403	organic carbon (photo-, mixo- and heterotrophic conditions)	390:448	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	12	43	dep	biomass	1883:1889	arg1	mg					1876:1877	mg	1876:1877	mg	1876:1877	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	2	44	theme	gracilis	322:329	arg1	composition					304:314	The cell composition	295:314	The cell composition of E. gracilis	295:329	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	7	45	dep	%	1279:1279	arg1	50					1277:1278	50	1277:1278	50	1277:1278	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	12	46	dep	mg	1876:1877	arg1	0.5					1872:1874	0.5	1872:1874	0.5	1872:1874	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	4	47	theme	E.	717:718	arg1	gracilis					720:727	E. gracilis	717:727	E. gracilis	717:727	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	13	48	located	detected	1967:1974	arg1	biomass					1993:1999	1 to 5 mg g-1 biomass	1979:1999	1 to 5 mg g-1 biomass	1979:1999	Eicosapentaenoic and docosahexaenoic acids were detected at 1 to 5 mg g-1 biomass.					
29649233	13	48	located	detected	1967:1974	arg2	acids					1956:1960	Eicosapentaenoic and docosahexaenoic acids	1919:1960	Eicosapentaenoic and docosahexaenoic acids	1919:1960	Eicosapentaenoic and docosahexaenoic acids were detected at 1 to 5 mg g-1 biomass.					
29649233	5	49	theme	growth	941:946	arg1	rate					921:924	the rate	917:924	the rate of exponential growth in all cultures	917:962	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	11	50	contain	contained	1684:1692	arg1	cells					1678:1682	Heterotrophic cells	1664:1682	Heterotrophic cells	1664:1682	Heterotrophic cells contained less unsaturated fatty acids than phototrophic or mixotrophic cells.					
29649233	11	50	contain	contained	1684:1692	arg2	acids					1717:1721	less unsaturated fatty acids	1694:1721	less unsaturated fatty acids	1694:1721	Heterotrophic cells contained less unsaturated fatty acids than phototrophic or mixotrophic cells.					
29649233	8	51	theme	lipophilic	1350:1359	arg1	compounds					1361:1369	less than 20% lipophilic compounds	1336:1369	less than 20% lipophilic compounds	1336:1369	These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected.					
29649233	12	52	from	biomass	1883:1889	arg1	cells					1894:1898	cells	1894:1898	cells grown in the dark	1894:1916	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	12	52	from	biomass	1883:1889	arg1	present					1784:1790	present	1784:1790	present	1784:1790	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	14	53	theme	vitamin	2049:2055	arg1	E					2057:2057	vitamin E	2049:2057	vitamin E	2049:2057	Light was also important for the production of vitamin E and phytol.					
29649233	12	54	theme	g-1	1879:1881	arg1	biomass					1883:1889	< 0.5 mg g-1 biomass	1870:1889	< 0.5 mg g-1 biomass in cells grown in the dark	1870:1916	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	13	55	theme	g-1	1989:1991	arg1	biomass					1993:1999	1 to 5 mg g-1 biomass	1979:1999	1 to 5 mg g-1 biomass	1979:1999	Eicosapentaenoic and docosahexaenoic acids were detected at 1 to 5 mg g-1 biomass.					
29649233	12	56	dep	g-1	1806:1808	arg1	to					1797:1798	to	1797:1798	to	1797:1798	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	8	57	dep	33	1320:1321	arg1	to					1317:1318	to	1317:1318	to	1317:1318	These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected.					
29649233	7	58	theme	large	1165:1169	arg1	paramylon					1182:1190	paramylon	1182:1190	paramylon	1182:1190	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	7	58	theme	large	1165:1169	arg1	amounts					1171:1177	large amounts	1165:1177	large amounts of paramylon	1165:1190	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	2	59	theme	cell	299:302	arg1	composition					304:314	The cell composition	295:314	The cell composition of E. gracilis	295:329	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	5	60	from	growth	941:946	arg1	cultures					955:962	all cultures	951:962	all cultures	951:962	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	1	61	theme	unsaturated	177:187	arg1	acids					195:199	unsaturated fatty acids	177:199	unsaturated fatty acids	177:199	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	11	62	theme	unsaturated	1699:1709	arg1	acids					1717:1721	less unsaturated fatty acids	1694:1721	less unsaturated fatty acids	1694:1721	Heterotrophic cells contained less unsaturated fatty acids than phototrophic or mixotrophic cells.					
29649233	7	63	dep	paramylon	1281:1289	arg1	%					1279:1279	%	1279:1279	%	1279:1279	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	6	64	contain	had	1064:1066	arg2	effect					1073:1078	less effect	1068:1078	less effect	1068:1078	Temperature had less effect on cell composition.					
29649233	6	64	contain	had	1064:1066	arg1	Temperature					1052:1062	Temperature	1052:1062	Temperature	1052:1062	Temperature had less effect on cell composition.					
29649233	6	65	theme	cell	1083:1086	arg1	composition					1088:1098	cell composition	1083:1098	cell composition	1083:1098	Temperature had less effect on cell composition.					
29649233	5	66	theme	light-limited	1003:1015	arg1	growth					1017:1022	light-limited growth	1003:1022	light-limited growth in phototrophic conditions	1003:1049	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	1	67	theme	wax	202:204	arg1	esters					206:211	wax esters	202:211	wax esters	202:211	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	4	68	theme	exponential	863:873	arg1	growth					875:880	exponential growth	863:880	exponential growth	863:880	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	10	69	theme	fatty	1504:1508	arg1	similar					1568:1574	similar	1568:1574	similar	1568:1574	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	10	69	theme	fatty	1504:1508	arg1	lower					1624:1628	lower	1624:1628	lower	1624:1628	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	10	69	theme	fatty	1504:1508	arg1	content					1515:1521	The fatty acid content	1500:1521	The fatty acid content of cells grown in mixotrophic conditions	1500:1562	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	11	70	theme	phototrophic	1728:1739	arg1	cells					1756:1760	phototrophic or mixotrophic cells	1728:1760	phototrophic or mixotrophic cells	1728:1760	Heterotrophic cells contained less unsaturated fatty acids than phototrophic or mixotrophic cells.					
29649233	10	71	located	observed	1584:1591	arg1	cells					1609:1613	phototrophic cells	1596:1613	phototrophic cells	1596:1613	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	10	71	located	observed	1584:1591	arg2	that					1579:1582	that	1579:1582	that	1579:1582	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	1	72	theme	Euglena	115:121	arg1	protist					150:156	a photosynthetic protist	133:156	a photosynthetic protist	133:156	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	1	72	theme	Euglena	115:121	arg1	gracilis					123:130	BACKGROUND Euglena gracilis	104:130	BACKGROUND Euglena gracilis	104:130	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	5	73	theme	linear	977:982	arg1	rate					991:994	the linear growth rate	973:994	the linear growth rate	973:994	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	12	74	from	biomass	1814:1820	arg1	cells					1825:1829	cells	1825:1829	cells grown in the presence of light	1825:1860	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	12	74	from	biomass	1814:1820	arg1	present					1784:1790	present	1784:1790	present	1784:1790	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	13	75	dep	5	1984:1984	arg1	to					1981:1982	to	1981:1982	to	1981:1982	Eicosapentaenoic and docosahexaenoic acids were detected at 1 to 5 mg g-1 biomass.					
29649233	12	76	located	present	1784:1790	arg2	acid					1775:1778	α-Linolenic acid	1763:1778	α-Linolenic acid	1763:1778	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	12	76	located	present	1784:1790	arg1	biomass					1814:1820	5 to 18 mg g-1 dry biomass	1795:1820	5 to 18 mg g-1 dry biomass in cells grown in the presence of light	1795:1860	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	1	77	theme	photosynthetic	135:148	arg1	protist					150:156	a photosynthetic protist	133:156	a photosynthetic protist	133:156	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	1	77	theme	photosynthetic	135:148	arg1	gracilis					123:130	BACKGROUND Euglena gracilis	104:130	BACKGROUND Euglena gracilis	104:130	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	12	78	theme	g-1	1806:1808	arg1	biomass					1814:1820	5 to 18 mg g-1 dry biomass	1795:1820	5 to 18 mg g-1 dry biomass in cells grown in the presence of light	1795:1860	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	8	79	dep	protein	1324:1330	arg1	%					1322:1322	%	1322:1322	%	1322:1322	These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected.					
29649233	1	80	dep	along	258:262	arg1	with					264:267	with	264:267	with	264:267	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	11	81	theme	Heterotrophic	1664:1676	arg1	cells					1678:1682	Heterotrophic cells	1664:1682	Heterotrophic cells	1664:1682	Heterotrophic cells contained less unsaturated fatty acids than phototrophic or mixotrophic cells.					
29649233	5	82	from	growth	1017:1022	arg1	conditions					1040:1049	phototrophic conditions	1027:1049	phototrophic conditions	1027:1049	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	13	83	theme	Eicosapentaenoic	1919:1934	arg1	acids					1956:1960	Eicosapentaenoic and docosahexaenoic acids	1919:1960	Eicosapentaenoic and docosahexaenoic acids	1919:1960	Eicosapentaenoic and docosahexaenoic acids were detected at 1 to 5 mg g-1 biomass.					
29649233	9	84	theme	fatty	1441:1445	arg1	esters					1459:1464	fatty acid methyl esters	1441:1464	fatty acid methyl esters	1441:1464	The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated.					
29649233	11	85	theme	mixotrophic	1744:1754	arg1	cells					1756:1760	phototrophic or mixotrophic cells	1728:1760	phototrophic or mixotrophic cells	1728:1760	Heterotrophic cells contained less unsaturated fatty acids than phototrophic or mixotrophic cells.					
29649233	9	86	theme	methyl	1452:1457	arg1	esters					1459:1464	fatty acid methyl esters	1441:1464	fatty acid methyl esters	1441:1464	The biomass contained about 8% fatty acids (measured as fatty acid methyl esters), most of which were unsaturated.					
29649233	7	87	theme	E.	1127:1128	arg1	gracilis					1130:1137	E. gracilis	1127:1137	E. gracilis	1127:1137	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	4	88	theme	CELL	658:661	arg1	rates					686:690	CELL GROWTH Specific growth rates	658:690	CELL GROWTH Specific growth rates	658:690	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	3	89	theme	growth	593:598	arg1	effect					583:588	the effect	579:588	the effect of growth at higher temperatures (27 or 30 °C)	579:635	Comparisons were primarily carried out in cultures grown at 23 °C, but the effect of growth at higher temperatures (27 or 30 °C) was also considered.					
29649233	4	90	theme	Specific	670:677	arg1	rates					686:690	CELL GROWTH Specific growth rates	658:690	CELL GROWTH Specific growth rates	658:690	CELL GROWTH Specific growth rates were slightly lower when E. gracilis was grown on glucose in either heterotrophic or mixotrophic conditions than when grown photoautotrophically, although the duration of exponential growth was longer.					
29649233	2	91	theme	organic	390:396	arg1	conditions					438:447	photo-, mixo- and heterotrophic conditions	406:447	photo-, mixo- and heterotrophic conditions	406:447	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	2	91	theme	organic	390:396	arg1	carbon					398:403	organic carbon	390:403	organic carbon (photo-, mixo- and heterotrophic conditions)	390:448	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	1	92	theme	valuable	275:282	arg1	compounds					284:292	other valuable compounds	269:292	other valuable compounds	269:292	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
29649233	7	93	theme	phototrophic	1238:1249	arg1	cells					1251:1255	phototrophic cells	1238:1255	phototrophic cells	1238:1255	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	10	94	theme	mixotrophic	1541:1551	arg1	conditions					1553:1562	mixotrophic conditions	1541:1562	mixotrophic conditions	1541:1562	The fatty acid content of cells grown in mixotrophic conditions was similar to that observed in phototrophic cells, but was lower in cells grown heterotrophically.					
29649233	2	95	dep	growth	459:464	arg1	lipids					499:504	lipids	499:504	growth and cell composition (especially lipids)	459:505	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	14	96	theme	E	2057:2057	arg1	production					2035:2044	the production	2031:2044	the production of vitamin E and phytol	2031:2068	Light was also important for the production of vitamin E and phytol.					
29649233	7	97	dep	50	1277:1278	arg1	to					1274:1275	to	1274:1275	to	1274:1275	CELL COMPOSITION Although E. gracilis was not expected to store large amounts of paramylon when grown phototrophically, we observed that phototrophic cells could contain up to 50% paramylon.					
29649233	2	98	theme	E.	319:320	arg1	gracilis					322:329	E. gracilis	319:329	E. gracilis	319:329	The cell composition of E. gracilis was investigated in this study to understand how light and organic carbon (photo-, mixo- and heterotrophic conditions) affected growth and cell composition (especially lipids).					
29649233	8	99	dep	compounds	1361:1369	arg1	%					1348:1348	%	1348:1348	%	1348:1348	These cells contained up to 33% protein and less than 20% lipophilic compounds, as expected.					
29649233	5	100	theme	exponential	929:939	arg1	growth					941:946	exponential growth	929:946	exponential growth in all cultures	929:962	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	3	101	theme	higher	603:608	arg1	°C					633:634	27 or 30 °C	624:634	27 or 30 °C	624:634	Comparisons were primarily carried out in cultures grown at 23 °C, but the effect of growth at higher temperatures (27 or 30 °C) was also considered.					
29649233	3	101	theme	higher	603:608	arg1	temperatures					610:621	higher temperatures	603:621	higher temperatures (27 or 30 °C)	603:635	Comparisons were primarily carried out in cultures grown at 23 °C, but the effect of growth at higher temperatures (27 or 30 °C) was also considered.					
29649233	12	102	attach	present	1784:1790	arg2	acid					1775:1778	α-Linolenic acid	1763:1778	α-Linolenic acid	1763:1778	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	12	102	attach	present	1784:1790	arg1	biomass					1883:1889	< 0.5 mg g-1 biomass	1870:1889	< 0.5 mg g-1 biomass in cells grown in the dark	1870:1916	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	12	102	attach	present	1784:1790	arg1	biomass					1814:1820	5 to 18 mg g-1 dry biomass	1795:1820	5 to 18 mg g-1 dry biomass in cells grown in the presence of light	1795:1860	α-Linolenic acid was present at 5 to 18 mg g-1 dry biomass in cells grown in the presence of light, but at < 0.5 mg g-1 biomass in cells grown in the dark.					
29649233	0	103	dep	gracilis	8:15	arg1	growth					17:22	growth	17:22	growth	17:22	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	0	103	dep	gracilis	8:15	arg1	composition					33:43	cell composition	28:43	cell composition	28:43	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	0	103	dep	gracilis	8:15	arg1	gracilis					8:15	Euglena gracilis growth and cell composition	0:43	Euglena gracilis growth and cell composition	0:43	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	0	103	dep	gracilis	8:15	arg1	conditions					92:101	different temperature, light and trophic conditions	51:101	different temperature, light and trophic conditions	51:101	Euglena gracilis growth and cell composition under different temperature, light and trophic conditions.					
29649233	3	104	from	temperatures	610:621	arg1	effect					583:588	the effect	579:588	the effect of growth at higher temperatures (27 or 30 °C)	579:635	Comparisons were primarily carried out in cultures grown at 23 °C, but the effect of growth at higher temperatures (27 or 30 °C) was also considered.					
29649233	5	105	from	rate	921:924	arg1	cultures					955:962	all cultures	951:962	all cultures	951:962	Temperature determined the rate of exponential growth in all cultures, but not the linear growth rate during light-limited growth in phototrophic conditions.					
29649233	1	106	theme	unique	220:225	arg1	β-1,3-glucan					227:238	a unique β-1,3-glucan	218:238	a unique β-1,3-glucan called paramylon, along with other valuable compounds	218:292	BACKGROUND Euglena gracilis, a photosynthetic protist, produces protein, unsaturated fatty acids, wax esters, and a unique β-1,3-glucan called paramylon, along with other valuable compounds.					
30201564	2	0	theme	homogenization	463:476	arg1	technique					484:492	hot high-pressure homogenization (HPH) technique	445:492	hot high-pressure homogenization (HPH) technique	445:492	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	1	1	theme	mucoadhesive	293:304	arg1	neuronanoemulsions					306:323	mucoadhesive neuronanoemulsions	293:323	mucoadhesive neuronanoemulsions (mNNEs)	293:331	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	1	1	theme	mucoadhesive	293:304	arg1	mNNEs					326:330	mNNEs	326:330	mNNEs	326:330	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	5	2	theme	nasal	731:735	arg1	mucosa					737:742	the nasal mucosa	727:742	the nasal mucosa	727:742	Here, an objective to provide controlled drug release with prolonged residence on the nasal mucosa for the treatment of Parkinson's disease (PD) is at prime consideration.					
30201564	6	3	theme	brain	826:830	arg1	targeting					832:840	Enhanced brain targeting	817:840	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane,	817:980	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	8	4	theme	intranasal	1231:1240	arg1	route					1242:1246	intranasal route	1231:1246	intranasal route	1231:1246	The mNNE formulation was administered to the Swiss albino mice model via intranasal route and both, the plasma and brain pharmacokinetics were estimated.					
30201564	4	5	theme	modified	564:571	arg1	mNNE					573:576	TMC modified mNNE	560:576	TMC modified mNNE	560:576	TMC modified mNNE have not been prepared yet for direct nose to brain drug delivery.					
30201564	2	6	theme	high-pressure	449:461	arg1	technique					484:492	hot high-pressure homogenization (HPH) technique	445:492	hot high-pressure homogenization (HPH) technique	445:492	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	6	7	theme	drug	861:864	arg1	delivery					866:873	drug delivery	861:873	drug delivery	861:873	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	1	8	theme	flaxseed	144:151	arg1	NNEs					183:186	NNEs	183:186	NNEs	183:186	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	1	8	theme	flaxseed	144:151	arg1	neuronanoemulsions					163:180	flaxseed oil-based neuronanoemulsions	144:180	flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs)	144:331	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	4	9	theme	TMC	560:562	arg1	mNNE					573:576	TMC modified mNNE	560:576	TMC modified mNNE	560:576	TMC modified mNNE have not been prepared yet for direct nose to brain drug delivery.					
30201564	0	10	theme	direct	89:94	arg1	nose					96:99	direct nose	89:99	direct nose to brain drug delivery	89:122	N,N,N‑trimethyl chitosan modified flaxseed oil based mucoadhesive neuronanoemulsions for direct nose to brain drug delivery.					
30201564	4	11	theme	drug	630:633	arg1	delivery					635:642	brain drug delivery	624:642	brain drug delivery	624:642	TMC modified mNNE have not been prepared yet for direct nose to brain drug delivery.					
30201564	9	12	dep	in	1316:1317	arg1	vivo					1319:1322	vivo	1319:1322	vivo	1319:1322	The in vivo studies performed on mice exhibited high brain targeting efficiency of mNNE formulation through nose to brain delivery via olfactory pathway.					
30201564	3	13	theme	Central	521:527	arg1	design					552:557	Central Composite experimental design	521:557	Central Composite experimental design	521:557	NNEs were optimized using Central Composite experimental design.					
30201564	9	14	theme	brain	1428:1432	arg1	delivery					1434:1441	brain delivery	1428:1441	brain delivery via olfactory pathway	1428:1463	The in vivo studies performed on mice exhibited high brain targeting efficiency of mNNE formulation through nose to brain delivery via olfactory pathway.					
30201564	5	15	theme	prime	796:800	arg1	consideration					802:814	prime consideration	796:814	prime consideration	796:814	Here, an objective to provide controlled drug release with prolonged residence on the nasal mucosa for the treatment of Parkinson's disease (PD) is at prime consideration.					
30201564	5	16	theme	prolonged	704:712	arg1	residence					714:722	prolonged residence	704:722	prolonged residence	704:722	Here, an objective to provide controlled drug release with prolonged residence on the nasal mucosa for the treatment of Parkinson's disease (PD) is at prime consideration.					
30201564	9	17	theme	mNNE	1395:1398	arg1	formulation					1400:1410	mNNE formulation	1395:1410	mNNE formulation	1395:1410	The in vivo studies performed on mice exhibited high brain targeting efficiency of mNNE formulation through nose to brain delivery via olfactory pathway.					
30201564	1	18	theme	oil-based	153:161	arg1	NNEs					183:186	NNEs	183:186	NNEs	183:186	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	1	18	theme	oil-based	153:161	arg1	neuronanoemulsions					163:180	flaxseed oil-based neuronanoemulsions	144:180	flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs)	144:331	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	6	19	theme	formulation	941:951	arg1	retention					923:931	enhanced retention	914:931	enhanced retention of mNNE formulation	914:951	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	6	20	theme	mucosal	964:970	arg1	membrane					972:979	nasal mucosal membrane	958:979	nasal mucosal membrane	958:979	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	4	21	theme	brain	624:628	arg1	delivery					635:642	brain drug delivery	624:642	brain drug delivery	624:642	TMC modified mNNE have not been prepared yet for direct nose to brain drug delivery.					
30201564	7	22	theme	stability	1136:1144	arg1	assessment					1146:1155	6 month stability assessment	1128:1155	6 month stability assessment	1128:1155	The mNNE formulation was subjected to 6 month stability assessment.					
30201564	0	23	theme	mucoadhesive	53:64	arg1	neuronanoemulsions					66:83	mucoadhesive neuronanoemulsions	53:83	mucoadhesive neuronanoemulsions for direct nose to brain drug delivery	53:122	N,N,N‑trimethyl chitosan modified flaxseed oil based mucoadhesive neuronanoemulsions for direct nose to brain drug delivery.					
30201564	6	24	theme	mNNE	936:939	arg1	formulation					941:951	mNNE formulation	936:951	mNNE formulation	936:951	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	8	25	theme	mice	1216:1219	arg1	model					1221:1225	the Swiss albino mice model	1199:1225	the Swiss albino mice model	1199:1225	The mNNE formulation was administered to the Swiss albino mice model via intranasal route and both, the plasma and brain pharmacokinetics were estimated.					
30201564	5	26	theme	disease	777:783	arg1	treatment					752:760	the treatment	748:760	the treatment of Parkinson's disease (PD)	748:788	Here, an objective to provide controlled drug release with prolonged residence on the nasal mucosa for the treatment of Parkinson's disease (PD) is at prime consideration.					
30201564	0	27	theme	drug	110:113	arg1	delivery					115:122	brain drug delivery	104:122	brain drug delivery	104:122	N,N,N‑trimethyl chitosan modified flaxseed oil based mucoadhesive neuronanoemulsions for direct nose to brain drug delivery.					
30201564	9	28	theme	formulation	1400:1410	arg1	efficiency					1381:1390	high brain targeting efficiency	1360:1390	high brain targeting efficiency of mNNE formulation	1360:1410	The in vivo studies performed on mice exhibited high brain targeting efficiency of mNNE formulation through nose to brain delivery via olfactory pathway.					
30201564	3	29	theme	Composite	529:537	arg1	design					552:557	Central Composite experimental design	521:557	Central Composite experimental design	521:557	NNEs were optimized using Central Composite experimental design.					
30201564	2	30	theme	sulfate	397:403	arg1	nanoplex					415:422	high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex	360:422	high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex	360:422	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	7	31	theme	6 month	1128:1134	arg1	assessment					1146:1155	6 month stability assessment	1128:1155	6 month stability assessment	1128:1155	The mNNE formulation was subjected to 6 month stability assessment.					
30201564	1	32	theme	N‑trimethyl	258:268	arg1	TMC					280:282	TMC	280:282	TMC	280:282	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	1	32	theme	N‑trimethyl	258:268	arg1	chitosan					270:277	N‑trimethyl chitosan	258:277	N‑trimethyl chitosan (TMC)	258:283	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	1	32	theme	N‑trimethyl	258:268	arg1	N					254:254	N	254:254	N	254:254	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	0	33	theme	brain	104:108	arg1	delivery					115:122	brain drug delivery	104:122	brain drug delivery	104:122	N,N,N‑trimethyl chitosan modified flaxseed oil based mucoadhesive neuronanoemulsions for direct nose to brain drug delivery.					
30201564	6	34	theme	Enhanced	817:824	arg1	targeting					832:840	Enhanced brain targeting	817:840	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane,	817:980	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	3	35	theme	experimental	539:550	arg1	design					552:557	Central Composite experimental design	521:557	Central Composite experimental design	521:557	NNEs were optimized using Central Composite experimental design.					
30201564	2	36	theme	ropinirole-dextran	378:395	arg1	ROPI-DS					406:412	ROPI-DS	406:412	ROPI-DS	406:412	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	2	36	theme	ropinirole-dextran	378:395	arg1	sulfate					397:403	partitioning ropinirole-dextran sulfate	365:403	high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex	360:422	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	2	37	theme	hot	445:447	arg1	technique					484:492	hot high-pressure homogenization (HPH) technique	445:492	hot high-pressure homogenization (HPH) technique	445:492	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	6	38	theme	enhanced	914:921	arg1	retention					923:931	enhanced retention	914:931	enhanced retention of mNNE formulation	914:951	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	5	39	theme	controlled	675:684	arg1	release					691:697	controlled drug release	675:697	controlled drug release with prolonged residence	675:722	Here, an objective to provide controlled drug release with prolonged residence on the nasal mucosa for the treatment of Parkinson's disease (PD) is at prime consideration.					
30201564	2	40	theme	partitioning	365:376	arg1	ROPI-DS					406:412	ROPI-DS	406:412	ROPI-DS	406:412	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	2	40	theme	partitioning	365:376	arg1	sulfate					397:403	partitioning ropinirole-dextran sulfate	365:403	high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex	360:422	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	0	41	theme	N‑trimethyl	4:14	arg1	chitosan					16:23	N‑trimethyl chitosan	4:23	N‑trimethyl chitosan	4:23	N,N,N‑trimethyl chitosan modified flaxseed oil based mucoadhesive neuronanoemulsions for direct nose to brain drug delivery.					
30201564	0	41	theme	N‑trimethyl	4:14	arg1	N					0:0	N	0:0	N	0:0	N,N,N‑trimethyl chitosan modified flaxseed oil based mucoadhesive neuronanoemulsions for direct nose to brain drug delivery.					
30201564	5	42	theme	drug	686:689	arg1	release					691:697	controlled drug release	675:697	controlled drug release with prolonged residence	675:722	Here, an objective to provide controlled drug release with prolonged residence on the nasal mucosa for the treatment of Parkinson's disease (PD) is at prime consideration.					
30201564	6	43	theme	nasal	958:962	arg1	membrane					972:979	nasal mucosal membrane	958:979	nasal mucosal membrane	958:979	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	9	44	theme	high	1360:1363	arg1	efficiency					1381:1390	high brain targeting efficiency	1360:1390	high brain targeting efficiency of mNNE formulation	1360:1410	The in vivo studies performed on mice exhibited high brain targeting efficiency of mNNE formulation through nose to brain delivery via olfactory pathway.					
30201564	9	45	theme	targeting	1371:1379	arg1	efficiency					1381:1390	high brain targeting efficiency	1360:1390	high brain targeting efficiency of mNNE formulation	1360:1410	The in vivo studies performed on mice exhibited high brain targeting efficiency of mNNE formulation through nose to brain delivery via olfactory pathway.					
30201564	2	46	theme	high	360:363	arg1	nanoplex					415:422	high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex	360:422	high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex	360:422	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	0	47	theme	flaxseed	34:41	arg1	oil					43:45	flaxseed oil	34:45	flaxseed oil based mucoadhesive neuronanoemulsions for direct nose to brain drug delivery	34:122	N,N,N‑trimethyl chitosan modified flaxseed oil based mucoadhesive neuronanoemulsions for direct nose to brain drug delivery.					
30201564	5	48	with	release	691:697	arg1	residence					714:722	prolonged residence	704:722	prolonged residence	704:722	Here, an objective to provide controlled drug release with prolonged residence on the nasal mucosa for the treatment of Parkinson's disease (PD) is at prime consideration.					
30201564	10	49	dep	more	1537:1540	arg1	studies					1575:1581	studies	1575:1581	studies	1575:1581	The prepared intranasal mNNEs could be on the clinics, if investigated more for behavioral and neurotoxicity studies.					
30201564	6	50	theme	therapeutic	885:895	arg1	efficacy					897:904	improved therapeutic efficacy	876:904	improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane	876:979	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	6	50	theme	therapeutic	885:895	arg1	BBB					850:852	BBB	850:852	BBB bypass drug delivery	850:873	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	10	51	theme	prepared	1470:1477	arg1	mNNEs					1490:1494	The prepared intranasal mNNEs	1466:1494	The prepared intranasal mNNEs	1466:1494	The prepared intranasal mNNEs could be on the clinics, if investigated more for behavioral and neurotoxicity studies.					
30201564	6	52	theme	improved	876:883	arg1	efficacy					897:904	improved therapeutic efficacy	876:904	improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane	876:979	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	6	52	theme	improved	876:883	arg1	BBB					850:852	BBB	850:852	BBB bypass drug delivery	850:873	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	9	53	theme	in	1316:1317	arg1	studies					1324:1330	The in vivo studies	1312:1330	The in vivo studies performed on mice	1312:1348	The in vivo studies performed on mice exhibited high brain targeting efficiency of mNNE formulation through nose to brain delivery via olfactory pathway.					
30201564	6	54	theme	experiment	1078:1087	arg1	outcomes					1061:1068	outcomes	1061:1068	outcomes of this experiment	1061:1087	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	10	55	theme	intranasal	1479:1488	arg1	mNNEs					1490:1494	The prepared intranasal mNNEs	1466:1494	The prepared intranasal mNNEs	1466:1494	The prepared intranasal mNNEs could be on the clinics, if investigated more for behavioral and neurotoxicity studies.					
30201564	6	56	theme	administration	1012:1025	arg1	frequency					999:1007	frequency	999:1007	frequency	999:1007	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	6	56	theme	administration	1012:1025	arg1	dose					990:993	dose	990:993	dose	990:993	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	8	57	theme	plasma	1262:1267	arg1	both					1252:1255	both	1252:1255	both	1252:1255	The mNNE formulation was administered to the Swiss albino mice model via intranasal route and both, the plasma and brain pharmacokinetics were estimated.					
30201564	8	57	theme	plasma	1262:1267	arg1	pharmacokinetics					1279:1294	the plasma and brain pharmacokinetics	1258:1294	pharmacokinetics	1279:1294	The mNNE formulation was administered to the Swiss albino mice model via intranasal route and both, the plasma and brain pharmacokinetics were estimated.					
30201564	2	58	theme	HPH	479:481	arg1	technique					484:492	hot high-pressure homogenization (HPH) technique	445:492	hot high-pressure homogenization (HPH) technique	445:492	The NNEs were loaded with high partitioning ropinirole-dextran sulfate (ROPI-DS) nanoplex and fabricated using hot high-pressure homogenization (HPH) technique.					
30201564	9	59	theme	olfactory	1447:1455	arg1	pathway					1457:1463	olfactory pathway	1447:1463	olfactory pathway	1447:1463	The in vivo studies performed on mice exhibited high brain targeting efficiency of mNNE formulation through nose to brain delivery via olfactory pathway.					
30201564	4	60	theme	direct	609:614	arg1	nose					616:619	direct nose	609:619	direct nose to brain drug delivery	609:642	TMC modified mNNE have not been prepared yet for direct nose to brain drug delivery.					
30201564	8	61	theme	brain	1273:1277	arg1	both					1252:1255	both	1252:1255	both	1252:1255	The mNNE formulation was administered to the Swiss albino mice model via intranasal route and both, the plasma and brain pharmacokinetics were estimated.					
30201564	8	61	theme	brain	1273:1277	arg1	pharmacokinetics					1279:1294	the plasma and brain pharmacokinetics	1258:1294	pharmacokinetics	1279:1294	The mNNE formulation was administered to the Swiss albino mice model via intranasal route and both, the plasma and brain pharmacokinetics were estimated.					
30201564	8	62	theme	Swiss	1203:1207	arg1	model					1221:1225	the Swiss albino mice model	1199:1225	the Swiss albino mice model	1199:1225	The mNNE formulation was administered to the Swiss albino mice model via intranasal route and both, the plasma and brain pharmacokinetics were estimated.					
30201564	6	63	dep	BBB	850:852	arg1	bypass					854:859	bypass	854:859	bypass drug delivery	854:873	Enhanced brain targeting through BBB bypass drug delivery, improved therapeutic efficacy through enhanced retention of mNNE formulation over nasal mucosal membrane, reduced dose and frequency of administration, and safety were further expected outcomes of this experiment.					
30201564	7	64	theme	mNNE	1094:1097	arg1	formulation					1099:1109	The mNNE formulation	1090:1109	The mNNE formulation	1090:1109	The mNNE formulation was subjected to 6 month stability assessment.					
30201564	8	65	theme	mNNE	1162:1165	arg1	formulation					1167:1177	The mNNE formulation	1158:1177	The mNNE formulation	1158:1177	The mNNE formulation was administered to the Swiss albino mice model via intranasal route and both, the plasma and brain pharmacokinetics were estimated.					
30201564	9	66	theme	brain	1365:1369	arg1	efficiency					1381:1390	high brain targeting efficiency	1360:1390	high brain targeting efficiency of mNNE formulation	1360:1410	The in vivo studies performed on mice exhibited high brain targeting efficiency of mNNE formulation through nose to brain delivery via olfactory pathway.					
30201564	1	67	theme	mucoadhesive	232:243	arg1	N					254:254	N	254:254	N	254:254	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
30201564	1	67	theme	mucoadhesive	232:243	arg1	polymer					245:251	a mucoadhesive polymer	230:251	a mucoadhesive polymer	230:251	Here we fabricated flaxseed oil-based neuronanoemulsions (NNEs) which were further surface-modified with a mucoadhesive polymer, N,N,N‑trimethyl chitosan (TMC) to form mucoadhesive neuronanoemulsions (mNNEs).					
29766500	0	0	theme	lipophilic	65:74	arg1	encapsulation					95:107	lipophilic bioactive nutrient encapsulation	65:107	lipophilic bioactive nutrient encapsulation	65:107	Konjac glucomannan octenyl succinate (KGOS) as an emulsifier for lipophilic bioactive nutrient encapsulation.					
29766500	6	1	theme	0.4	918:920	arg1	mg					922:923	mg	922:923	mg	922:923	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	2	2	theme	in	341:342	arg1	properties					360:369	in vitro digestion properties	341:369	in vitro digestion properties	341:369	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	5	3	theme	representative	789:802	arg1	compound					825:832	a representative lipophilic bioactive compound	787:832	a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS	787:887	β-Carotene was selected as a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS.					
29766500	5	3	theme	representative	789:802	arg1	β-Carotene					760:769	β-Carotene	760:769	β-Carotene	760:769	β-Carotene was selected as a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS.					
29766500	6	4	dep	particles	1041:1049	arg1	self-aggregate					1026:1039	self-aggregate	1026:1039	self-aggregate	1026:1039	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	7	5	theme	0.3	1147:1149	arg1	%					1150:1150	%	1150:1150	%	1150:1150	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	6	6	theme	loading	894:900	arg1	capacity					902:909	The loading capacity	890:909	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene	890:957	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	6	6	theme	loading	894:900	arg1	%					967:967	3.26%	963:967	3.26%	963:967	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	9	7	dep	CONCLUSION	1520:1529	arg1	suggested					1557:1565	suggested	1557:1565	suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation	1557:1669	CONCLUSION The results of this study suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation.					
29766500	10	8	theme	Chemical	1690:1697	arg1	Industry					1699:1706	Chemical Industry	1690:1706	Chemical Industry	1690:1706	© 2018 Society of Chemical Industry.					
29766500	0	9	theme	nutrient	86:93	arg1	encapsulation					95:107	lipophilic bioactive nutrient encapsulation	65:107	lipophilic bioactive nutrient encapsulation	65:107	Konjac glucomannan octenyl succinate (KGOS) as an emulsifier for lipophilic bioactive nutrient encapsulation.					
29766500	6	10	theme	solution	935:942	arg1	capacity					902:909	The loading capacity	890:909	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene	890:957	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	6	10	theme	solution	935:942	arg1	%					967:967	3.26%	963:967	3.26%	963:967	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	7	11	theme	%	1133:1133	arg1	β-carotene					1135:1144	0.03% β-carotene	1129:1144	0.03% β-carotene	1129:1144	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	2	12	dep	in	341:342	arg1	vitro					344:348	vitro	344:348	vitro	344:348	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	0	13	theme	bioactive	76:84	arg1	encapsulation					95:107	lipophilic bioactive nutrient encapsulation	65:107	lipophilic bioactive nutrient encapsulation	65:107	Konjac glucomannan octenyl succinate (KGOS) as an emulsifier for lipophilic bioactive nutrient encapsulation.					
29766500	6	14	theme	KGOS	930:933	arg1	solution					935:942	the 0.4 mg mL-1 KGOS solution	914:942	the 0.4 mg mL-1 KGOS solution	914:942	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	7	15	theme	β-carotene	1135:1144	arg1	composition					1114:1124	a composition	1112:1124	a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides	1112:1190	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	7	16	with	%	1260:1260	arg1	composition					1114:1124	a composition	1112:1124	a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides	1112:1190	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	3	17	theme	laser	566:570	arg1	CSLM					584:587	CSLM	584:587	CSLM	584:587	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	3	17	theme	laser	566:570	arg1	microscopy					572:581	Confocal scanning laser microscopy	548:581	RESULTS Confocal scanning laser microscopy (CSLM)	540:588	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	2	18	theme	bioactive	519:527	arg1	nutrients					529:537	insoluble bioactive nutrients	509:537	insoluble bioactive nutrients	509:537	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	3	19	dep	RESULTS	540:546	arg1	CSLM					584:587	CSLM	584:587	CSLM	584:587	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	3	19	dep	RESULTS	540:546	arg1	microscopy					572:581	Confocal scanning laser microscopy	548:581	RESULTS Confocal scanning laser microscopy (CSLM)	540:588	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	9	20	theme	bioactive	1638:1646	arg1	encapsulation					1657:1669	lipophilic bioactive nutrient encapsulation	1627:1669	lipophilic bioactive nutrient encapsulation	1627:1669	CONCLUSION The results of this study suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation.					
29766500	2	21	with	KGOS	499:502	arg1	nutrients					529:537	insoluble bioactive nutrients	509:537	insoluble bioactive nutrients	509:537	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	8	22	theme	KGOSC	1339:1343	arg1	nanoemulsion					1345:1356	the KGOSC nanoemulsion	1335:1356	the KGOSC nanoemulsion	1335:1356	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	4	23	theme	digestion	679:687	arg1	properties					689:698	In vitro digestion properties	670:698	In vitro digestion properties	670:698	In vitro digestion properties showed that KGOS is effective for colon-targeted transport.					
29766500	4	24	theme	In	670:671	arg1	properties					689:698	In vitro digestion properties	670:698	In vitro digestion properties	670:698	In vitro digestion properties showed that KGOS is effective for colon-targeted transport.					
29766500	2	25	theme	insoluble	509:517	arg1	nutrients					529:537	insoluble bioactive nutrients	509:537	insoluble bioactive nutrients	509:537	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	6	26	theme	mg	922:923	arg1	mL-1					925:928	the 0.4 mg mL-1	914:928	the 0.4 mg mL-1 KGOS solution	914:942	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	5	27	theme	lipophilic	804:813	arg1	compound					825:832	a representative lipophilic bioactive compound	787:832	a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS	787:887	β-Carotene was selected as a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS.					
29766500	5	27	theme	lipophilic	804:813	arg1	β-Carotene					760:769	β-Carotene	760:769	β-Carotene	760:769	β-Carotene was selected as a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS.					
29766500	8	28	theme	storage	1280:1286	arg1	days					1272:1275	30 days	1269:1275	30 days of storage	1269:1286	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	3	29	theme	Confocal	548:555	arg1	CSLM					584:587	CSLM	584:587	CSLM	584:587	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	3	29	theme	Confocal	548:555	arg1	microscopy					572:581	Confocal scanning laser microscopy	548:581	RESULTS Confocal scanning laser microscopy (CSLM)	540:588	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	1	30	theme	excellent	169:177	arg1	properties					194:203	excellent emulsification properties	169:203	excellent emulsification properties	169:203	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	6	31	theme	transmission	974:985	arg1	microscopy					996:1005	transmission electron microscopy	974:1005	transmission electron microscopy	974:1005	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	7	32	theme	KGOSC	1225:1229	arg1	nanoemulsion					1231:1242	the KGOSC nanoemulsion	1221:1242	the KGOSC nanoemulsion	1221:1242	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	6	33	theme	mL-1	925:928	arg1	solution					935:942	the 0.4 mg mL-1 KGOS solution	914:942	the 0.4 mg mL-1 KGOS solution	914:942	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	1	34	theme	emulsification	179:192	arg1	properties					194:203	excellent emulsification properties	169:203	excellent emulsification properties	169:203	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	7	35	dep	β-carotene	1135:1144	arg1	triglycerides					1178:1190	medium-chain triglycerides	1165:1190	medium-chain triglycerides	1165:1190	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	8	36	theme	storage	1447:1453	arg1	conditions					1455:1464	storage conditions	1447:1464	storage conditions	1447:1464	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	8	37	theme	nanoemulsion	1345:1356	arg1	size					1302:1305	the particle size	1289:1305	the particle size	1289:1305	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	8	37	theme	nanoemulsion	1345:1356	arg1	nm					1375:1376	less than 5 nm	1363:1376	less than 5 nm	1363:1376	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	8	37	theme	nanoemulsion	1345:1356	arg1	polydispersity					1311:1324	polydispersity	1311:1324	polydispersity	1311:1324	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	5	38	theme	emulsification	850:863	arg1	characteristics					865:879	the emulsification characteristics	846:879	the emulsification characteristics of KGOS	846:887	β-Carotene was selected as a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS.					
29766500	9	39	theme	potential	1587:1595	arg1	KGOS					1572:1575	KGOS	1572:1575	KGOS	1572:1575	CONCLUSION The results of this study suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation.					
29766500	9	39	theme	potential	1587:1595	arg1	emulsifier					1597:1606	a good potential emulsifier	1580:1606	a good potential emulsifier	1580:1606	CONCLUSION The results of this study suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation.					
29766500	2	40	theme	KGOS	418:421	arg1	stability					405:413	stability	405:413	stability	405:413	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	2	40	theme	KGOS	418:421	arg1	properties					360:369	in vitro digestion properties	341:369	in vitro digestion properties	341:369	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	2	40	theme	KGOS	418:421	arg1	capability					390:399	emulsification capability	375:399	emulsification capability	375:399	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	0	41	theme	octenyl	19:25	arg1	KGOS					38:41	KGOS	38:41	KGOS	38:41	Konjac glucomannan octenyl succinate (KGOS) as an emulsifier for lipophilic bioactive nutrient encapsulation.					
29766500	0	41	theme	octenyl	19:25	arg1	succinate					27:35	octenyl succinate	19:35	octenyl succinate (KGOS)	19:42	Konjac glucomannan octenyl succinate (KGOS) as an emulsifier for lipophilic bioactive nutrient encapsulation.					
29766500	0	41	theme	octenyl	19:25	arg1	emulsifier					50:59	an emulsifier	47:59	an emulsifier for lipophilic bioactive nutrient encapsulation	47:107	Konjac glucomannan octenyl succinate (KGOS) as an emulsifier for lipophilic bioactive nutrient encapsulation.					
29766500	6	42	theme	electron	987:994	arg1	microscopy					996:1005	transmission electron microscopy	974:1005	transmission electron microscopy	974:1005	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	3	43	theme	regular	653:659	arg1	spheres					661:667	regular spheres	653:667	regular spheres	653:667	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	9	44	theme	study	1551:1555	arg1	results					1535:1541	The results	1531:1541	The results of this study	1531:1555	CONCLUSION The results of this study suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation.					
29766500	5	45	theme	KGOS	884:887	arg1	characteristics					865:879	the emulsification characteristics	846:879	the emulsification characteristics of KGOS	846:887	β-Carotene was selected as a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS.					
29766500	2	46	theme	encapsulation	466:478	arg1	characteristics					480:494	the transport and encapsulation characteristics	448:494	characteristics	480:494	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	1	47	theme	microcapsule	287:298	arg1	material					305:312	microcapsule wall material	287:312	microcapsule wall material	287:312	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	47	theme	microcapsule	287:298	arg1	succinate					148:156	BACKGROUND Konjac glucomannan octenyl succinate	110:156	BACKGROUND Konjac glucomannan octenyl succinate (KGOS)	110:163	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	3	48	theme	oil	605:607	arg1	droplets					609:616	oil droplets	605:616	oil droplets	605:616	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	7	49	theme	%	1150:1150	arg1	KGOS					1152:1155	0.3% KGOS	1147:1155	0.3% KGOS	1147:1155	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	5	50	theme	bioactive	815:823	arg1	compound					825:832	a representative lipophilic bioactive compound	787:832	a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS	787:887	β-Carotene was selected as a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS.					
29766500	5	50	theme	bioactive	815:823	arg1	β-Carotene					760:769	β-Carotene	760:769	β-Carotene	760:769	β-Carotene was selected as a representative lipophilic bioactive compound to evaluate the emulsification characteristics of KGOS.					
29766500	1	51	theme	wall	300:303	arg1	material					305:312	microcapsule wall material	287:312	microcapsule wall material	287:312	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	51	theme	wall	300:303	arg1	succinate					148:156	BACKGROUND Konjac glucomannan octenyl succinate	110:156	BACKGROUND Konjac glucomannan octenyl succinate (KGOS)	110:163	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	9	52	theme	good	1582:1585	arg1	KGOS					1572:1575	KGOS	1572:1575	KGOS	1572:1575	CONCLUSION The results of this study suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation.					
29766500	9	52	theme	good	1582:1585	arg1	emulsifier					1597:1606	a good potential emulsifier	1580:1606	a good potential emulsifier	1580:1606	CONCLUSION The results of this study suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation.					
29766500	8	53	theme	KGOSC	1424:1428	arg1	nanoemulsions					1430:1442	KGOSC nanoemulsions	1424:1442	KGOSC nanoemulsions	1424:1442	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	7	54	theme	%	1163:1163	arg1	composition					1114:1124	a composition	1112:1124	a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides	1112:1190	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	7	55	theme	emulsification	1197:1210	arg1	yield					1212:1216	the emulsification yield	1193:1216	the emulsification yield of the KGOSC nanoemulsion	1193:1242	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	7	55	theme	emulsification	1197:1210	arg1	%					1260:1260	more than 95%	1248:1260	more than 95%	1248:1260	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	2	56	theme	transport	452:460	arg1	characteristics					480:494	the transport and encapsulation characteristics	448:494	characteristics	480:494	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	1	57	contain	has	165:167	arg1	material					305:312	microcapsule wall material	287:312	microcapsule wall material	287:312	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	57	contain	has	165:167	arg1	succinate					148:156	BACKGROUND Konjac glucomannan octenyl succinate	110:156	BACKGROUND Konjac glucomannan octenyl succinate (KGOS)	110:163	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	57	contain	has	165:167	arg1	KGOS					159:162	KGOS	159:162	KGOS	159:162	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	57	contain	has	165:167	arg1	stabilizer					272:281	stabilizer	272:281	stabilizer	272:281	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	57	contain	has	165:167	arg2	properties					194:203	excellent emulsification properties	169:203	excellent emulsification properties	169:203	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	57	contain	has	165:167	arg1	emulsifier					260:269	an emulsifier	257:269	an emulsifier	257:269	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	8	58	dep	size	1302:1305	arg1	index					1326:1330	index	1326:1330	index	1326:1330	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	1	59	theme	BACKGROUND	110:119	arg1	material					305:312	microcapsule wall material	287:312	microcapsule wall material	287:312	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	59	theme	BACKGROUND	110:119	arg1	KGOS					159:162	KGOS	159:162	KGOS	159:162	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	59	theme	BACKGROUND	110:119	arg1	stabilizer					272:281	stabilizer	272:281	stabilizer	272:281	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	59	theme	BACKGROUND	110:119	arg1	succinate					148:156	BACKGROUND Konjac glucomannan octenyl succinate	110:156	BACKGROUND Konjac glucomannan octenyl succinate (KGOS)	110:163	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	59	theme	BACKGROUND	110:119	arg1	emulsifier					260:269	an emulsifier	257:269	an emulsifier	257:269	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	7	60	theme	KGOS	1152:1155	arg1	composition					1114:1124	a composition	1112:1124	a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides	1112:1190	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	9	61	theme	nutrient	1648:1655	arg1	encapsulation					1657:1669	lipophilic bioactive nutrient encapsulation	1627:1669	lipophilic bioactive nutrient encapsulation	1627:1669	CONCLUSION The results of this study suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation.					
29766500	7	62	theme	0.03	1129:1132	arg1	%					1133:1133	%	1133:1133	%	1133:1133	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	9	63	theme	lipophilic	1627:1636	arg1	encapsulation					1657:1669	lipophilic bioactive nutrient encapsulation	1627:1669	lipophilic bioactive nutrient encapsulation	1627:1669	CONCLUSION The results of this study suggested that KGOS is a good potential emulsifier and stabilizer for lipophilic bioactive nutrient encapsulation.					
29766500	1	64	theme	Konjac	121:126	arg1	material					305:312	microcapsule wall material	287:312	microcapsule wall material	287:312	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	64	theme	Konjac	121:126	arg1	KGOS					159:162	KGOS	159:162	KGOS	159:162	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	64	theme	Konjac	121:126	arg1	stabilizer					272:281	stabilizer	272:281	stabilizer	272:281	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	64	theme	Konjac	121:126	arg1	succinate					148:156	BACKGROUND Konjac glucomannan octenyl succinate	110:156	BACKGROUND Konjac glucomannan octenyl succinate (KGOS)	110:163	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	64	theme	Konjac	121:126	arg1	emulsifier					260:269	an emulsifier	257:269	an emulsifier	257:269	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	4	65	theme	colon-targeted	734:747	arg1	transport					749:757	colon-targeted transport	734:757	colon-targeted transport	734:757	In vitro digestion properties showed that KGOS is effective for colon-targeted transport.					
29766500	8	66	theme	nanoemulsions	1430:1442	arg1	sensitivity					1409:1419	the sensitivity	1405:1419	the sensitivity of KGOSC nanoemulsions to storage conditions	1405:1464	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	1	67	theme	glucomannan	128:138	arg1	material					305:312	microcapsule wall material	287:312	microcapsule wall material	287:312	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	67	theme	glucomannan	128:138	arg1	KGOS					159:162	KGOS	159:162	KGOS	159:162	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	67	theme	glucomannan	128:138	arg1	stabilizer					272:281	stabilizer	272:281	stabilizer	272:281	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	67	theme	glucomannan	128:138	arg1	succinate					148:156	BACKGROUND Konjac glucomannan octenyl succinate	110:156	BACKGROUND Konjac glucomannan octenyl succinate (KGOS)	110:163	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	67	theme	glucomannan	128:138	arg1	emulsifier					260:269	an emulsifier	257:269	an emulsifier	257:269	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	2	68	dep	properties	360:369	arg1	the					337:339	the	337:339	the	337:339	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	1	69	theme	octenyl	140:146	arg1	material					305:312	microcapsule wall material	287:312	microcapsule wall material	287:312	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	69	theme	octenyl	140:146	arg1	KGOS					159:162	KGOS	159:162	KGOS	159:162	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	69	theme	octenyl	140:146	arg1	stabilizer					272:281	stabilizer	272:281	stabilizer	272:281	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	69	theme	octenyl	140:146	arg1	succinate					148:156	BACKGROUND Konjac glucomannan octenyl succinate	110:156	BACKGROUND Konjac glucomannan octenyl succinate (KGOS)	110:163	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	69	theme	octenyl	140:146	arg1	emulsifier					260:269	an emulsifier	257:269	an emulsifier	257:269	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	2	70	theme	present	322:328	arg1	study					330:334	the present study	318:334	the present study	318:334	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	7	71	theme	nanoemulsion	1231:1242	arg1	yield					1212:1216	the emulsification yield	1193:1216	the emulsification yield of the KGOSC nanoemulsion	1193:1242	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	7	71	theme	nanoemulsion	1231:1242	arg1	%					1260:1260	more than 95%	1248:1260	more than 95%	1248:1260	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	1	72	used	used	228:231	arg2	emulsifier					260:269	an emulsifier	257:269	an emulsifier	257:269	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	72	used	used	228:231	arg2	material					305:312	microcapsule wall material	287:312	microcapsule wall material	287:312	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	72	used	used	228:231	arg2	KGOS					159:162	KGOS	159:162	KGOS	159:162	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	72	used	used	228:231	arg2	succinate					148:156	BACKGROUND Konjac glucomannan octenyl succinate	110:156	BACKGROUND Konjac glucomannan octenyl succinate (KGOS)	110:163	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	1	72	used	used	228:231	arg2	stabilizer					272:281	stabilizer	272:281	stabilizer	272:281	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29766500	4	73	dep	In	670:671	arg1	vitro					673:677	vitro	673:677	vitro	673:677	In vitro digestion properties showed that KGOS is effective for colon-targeted transport.					
29766500	2	74	theme	digestion	350:358	arg1	properties					360:369	in vitro digestion properties	341:369	in vitro digestion properties	341:369	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	2	75	theme	emulsification	375:388	arg1	capability					390:399	emulsification capability	375:399	emulsification capability	375:399	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	6	76	theme	KGOS/β-carotene	1054:1068	arg1	particles					1041:1049	the self-aggregate particles	1022:1049	the self-aggregate particles of KGOS/β-carotene (KGOSC)	1022:1076	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	6	76	theme	KGOS/β-carotene	1054:1068	arg1	uniform					1088:1094	uniform	1088:1094	uniform	1088:1094	The loading capacity of the 0.4 mg mL-1 KGOS solution for β-carotene was 3.26%, and transmission electron microscopy suggested that the self-aggregate particles of KGOS/β-carotene (KGOSC) were more uniform than KGOS.					
29766500	2	77	theme	KGOS	499:502	arg1	characteristics					480:494	the transport and encapsulation characteristics	448:494	characteristics	480:494	In the present study, the in vitro digestion properties and emulsification capability and stability of KGOS were studied to evaluate the transport and encapsulation characteristics of KGOS with insoluble bioactive nutrients.					
29766500	3	78	theme	scanning	557:564	arg1	CSLM					584:587	CSLM	584:587	CSLM	584:587	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	3	78	theme	scanning	557:564	arg1	microscopy					572:581	Confocal scanning laser microscopy	548:581	RESULTS Confocal scanning laser microscopy (CSLM)	540:588	RESULTS Confocal scanning laser microscopy (CSLM) suggested that oil droplets could be encapsulated by KGOS into regular spheres.					
29766500	7	79	theme	medium-chain	1165:1176	arg1	triglycerides					1178:1190	medium-chain triglycerides	1165:1190	medium-chain triglycerides	1165:1190	With a composition of 0.03% β-carotene, 0.3% KGOS and 10% medium-chain triglycerides, the emulsification yield of the KGOSC nanoemulsion was more than 95%.					
29766500	8	80	theme	particle	1293:1300	arg1	size					1302:1305	the particle size	1289:1305	the particle size	1289:1305	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	8	80	theme	particle	1293:1300	arg1	nm					1375:1376	less than 5 nm	1363:1376	less than 5 nm	1363:1376	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	8	81	theme	order	1483:1487	arg1	temperature					1489:1499	the order temperature	1479:1499	the order temperature	1479:1499	After 30 days of storage, the particle size and polydispersity index of the KGOSC nanoemulsion were less than 5 nm and 0.5, respectively, and the sensitivity of KGOSC nanoemulsions to storage conditions decreased in the order temperature, oxygen and light.					
29766500	1	82	theme	food	240:243	arg1	industry					245:252	the food industry	236:252	the food industry	236:252	BACKGROUND Konjac glucomannan octenyl succinate (KGOS) has excellent emulsification properties and can potentially be used in the food industry as an emulsifier, stabilizer and microcapsule wall material.					
29455388	4	0	theme	heterologous	570:581	arg1	expression					583:592	heterologous expression	570:592	heterologous expression of hyaluronidase	570:609	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	4	0	theme	heterologous	570:581	arg1	tool					638:641	a biotechnological tool	619:641	a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies	619:729	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	10	1	theme	PNGase	1531:1536	arg1	F					1538:1538	PNGase F	1531:1538	PNGase F	1531:1538	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	9	2	theme	final	1405:1409	arg1	material					1421:1428	the final expressed material	1401:1428	the final expressed material	1401:1428	rTsHyal-1 partially purified through cation exchange chromatography presented a specific activity of 1097 TRU/mg, against 838 TRU/mg for the final expressed material, representing a 1.31-fold purification.					
29455388	7	3	theme	buffered	1098:1105	arg1	medium					1115:1120	a buffered minimal medium	1096:1120	a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol	1096:1187	rTsHyal-1 was expressed in laboratorial scale in a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol.					
29455388	2	4	theme	extracellular	291:303	arg1	matrix					305:310	the extracellular matrix	287:310	the extracellular matrix	287:310	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	5	5	dep	Tityus	796:801	arg1	serrulatus					803:812	serrulatus	803:812	serrulatus	803:812	Therefore, this study produced a recombinant hyaluronidase from Tityus serrulatus scorpion venom, designated as rTsHyal-1, in the Pichia pastoris system.					
29455388	1	6	theme	potential	168:176	arg1	application					178:188	a broad potential application	160:188	a broad potential application	160:188	In general, hyaluronidases have a broad potential application on medicine and esthetics fields.					
29455388	14	7	dep	P.	2160:2161	arg1	pastoris					2163:2170	pastoris	2163:2170	pastoris	2163:2170	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	4	8	theme	amounts	671:677	arg1	obtainment					650:659	the obtainment	646:659	the obtainment of enough amounts of the enzyme	646:691	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	7	9	theme	laboratorial	1074:1085	arg1	scale					1087:1091	laboratorial scale	1074:1091	laboratorial scale	1074:1091	rTsHyal-1 was expressed in laboratorial scale in a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol.					
29455388	9	10	theme	1.31-fold	1446:1454	arg1	purification					1456:1467	a 1.31-fold purification	1444:1467	a 1.31-fold purification	1444:1467	rTsHyal-1 partially purified through cation exchange chromatography presented a specific activity of 1097 TRU/mg, against 838 TRU/mg for the final expressed material, representing a 1.31-fold purification.					
29455388	7	11	with	96 h	1152:1155	arg1	addition					1168:1175	daily addition	1162:1175	daily addition of methanol	1162:1187	rTsHyal-1 was expressed in laboratorial scale in a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol.					
29455388	14	12	theme	enzyme	2194:2199	arg1	activity					2201:2208	preserved enzyme activity	2184:2208	preserved enzyme activity	2184:2208	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	11	13	theme	sequence	1763:1770	arg1	coverage					1772:1779	protein sequence coverage	1755:1779	protein sequence coverage	1755:1779	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	4	14	from	tool	638:641	arg1	obtainment					650:659	the obtainment	646:659	the obtainment of enough amounts of the enzyme	646:691	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	8	15	theme	0.266 mg/mL	1251:1261	arg1	yield					1242:1246	a total protein yield	1226:1246	a total protein yield of 0.266 mg/mL	1226:1261	Expression of rTsHyal-1 resulted in a total protein yield of 0.266 mg/mL.					
29455388	10	16	contain	has	1480:1482	arg1	rTsHyal-1					1470:1478	rTsHyal-1	1470:1478	rTsHyal-1	1470:1478	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	10	16	contain	has	1480:1482	arg2	mass					1494:1497	molecular mass	1484:1497	molecular mass of 49.5 kDa	1484:1509	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	8	17	theme	rTsHyal-1	1204:1212	arg1	Expression					1190:1199	Expression	1190:1199	Expression of rTsHyal-1	1190:1212	Expression of rTsHyal-1 resulted in a total protein yield of 0.266 mg/mL.					
29455388	10	18	theme	potential	1598:1606	arg1	N-glycosylation					1608:1622	a potential N-glycosylation	1596:1622	a potential N-glycosylation of 4.5 kDa	1596:1633	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	14	19	theme	recombinant	2096:2106	arg1	hyaluronidase					2108:2120	the first recombinant hyaluronidase	2086:2120	the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity	2086:2208	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	14	19	theme	recombinant	2096:2106	arg1	This					2078:2081	This	2078:2081	This	2078:2081	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	2	20	from	matrix	305:310	arg1	present					276:282	present	276:282	present	276:282	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	2	21	attach	present	276:282	arg1	matrix					305:310	the extracellular matrix	287:310	the extracellular matrix	287:310	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	2	21	attach	present	276:282	arg2	hyaluronan					265:274	hyaluronan	265:274	hyaluronan	265:274	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	14	22	theme	P.	2160:2161	arg1	system					2172:2177	the P. pastoris system	2156:2177	the P. pastoris system with preserved enzyme activity	2156:2208	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	0	23	theme	scorpion	77:84	arg1	venom					86:90	scorpion venom	77:90	scorpion venom	77:90	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system.					
29455388	3	24	from	characterization	405:420	arg1	venoms					451:456	animal venoms	444:456	animal venoms	444:456	However, the in-depth characterization of hyaluronidase from animal venoms has been neglected due to its instability and low concentration in the venom, which hamper its isolation.					
29455388	7	25	theme	methanol	1180:1187	arg1	addition					1168:1175	daily addition	1162:1175	daily addition of methanol	1162:1187	rTsHyal-1 was expressed in laboratorial scale in a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol.					
29455388	6	26	theme	pPICZαA	964:970	arg1	vector					972:977	the pPICZαA vector	960:977	the pPICZαA vector (GenScript Corporation) for heterologous expression in P. pastoris	960:1044	|Thus, a gene for TsHyal-1 (gb||KF623285.1) was synthesized and cloned into the pPICZαA vector (GenScript Corporation) for heterologous expression in P. pastoris.|					
29455388	6	26	theme	pPICZαA	964:970	arg1	Corporation					990:1000	Corporation	990:1000	Corporation	990:1000	|Thus, a gene for TsHyal-1 (gb||KF623285.1) was synthesized and cloned into the pPICZαA vector (GenScript Corporation) for heterologous expression in P. pastoris.|					
29455388	10	27	gly	N-glycosylation	1608:1622	arg1	4.5 kDa					1627:1633	4.5 kDa	1627:1633	4.5 kDa	1627:1633	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	3	28	theme	animal	444:449	arg1	venoms					451:456	animal venoms	444:456	animal venoms	444:456	However, the in-depth characterization of hyaluronidase from animal venoms has been neglected due to its instability and low concentration in the venom, which hamper its isolation.					
29455388	0	29	theme	Pichia	104:109	arg1	pastoris					111:118	Pichia pastoris	104:118	Pichia pastoris system	104:125	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system.					
29455388	9	30	theme	exchange	1308:1315	arg1	chromatography					1317:1330	cation exchange chromatography	1301:1330	cation exchange chromatography	1301:1330	rTsHyal-1 partially purified through cation exchange chromatography presented a specific activity of 1097 TRU/mg, against 838 TRU/mg for the final expressed material, representing a 1.31-fold purification.					
29455388	1	31	contain	have	155:158	arg1	hyaluronidases					140:153	hyaluronidases	140:153	hyaluronidases	140:153	In general, hyaluronidases have a broad potential application on medicine and esthetics fields.					
29455388	1	31	contain	have	155:158	arg2	application					178:188	a broad potential application	160:188	a broad potential application	160:188	In general, hyaluronidases have a broad potential application on medicine and esthetics fields.					
29455388	3	32	theme	hyaluronidase	425:437	arg1	characterization					405:420	the in-depth characterization	392:420	the in-depth characterization of hyaluronidase from animal venoms	392:456	However, the in-depth characterization of hyaluronidase from animal venoms has been neglected due to its instability and low concentration in the venom, which hamper its isolation.					
29455388	0	33	theme	Heterologous	0:11	arg1	expression					13:22	Heterologous expression	0:22	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom	0:90	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system.					
29455388	12	34	theme	dermatan	1911:1918	arg1	sulfate					1920:1926	dermatan sulfate	1911:1926	dermatan sulfate	1911:1926	rTsHyal-1 presents the highest substrate specificity to hyaluronan followed by chondroitin-6-sulfate, chondroitin-4-sulfate, and dermatan sulfate and showed an optimum activity at pH 6.0 and 40 °C.					
29455388	10	35	theme	49.5 kDa	1502:1509	arg1	mass					1494:1497	molecular mass	1484:1497	molecular mass of 49.5 kDa	1484:1509	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	9	36	theme	specific	1344:1351	arg1	activity					1353:1360	a specific activity	1342:1360	a specific activity of 1097 TRU/mg	1342:1375	rTsHyal-1 partially purified through cation exchange chromatography presented a specific activity of 1097 TRU/mg, against 838 TRU/mg for the final expressed material, representing a 1.31-fold purification.					
29455388	2	37	from	present	276:282	arg1	matrix					305:310	the extracellular matrix	287:310	the extracellular matrix	287:310	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	11	38	theme	MALDI-TOF	1696:1704	arg1	MS					1730:1731	MALDI-TOF and Q Exactive Orbitrap MS	1696:1731	MS	1730:1731	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	0	39	theme	first	42:46	arg1	hyaluronidase					60:72	the first recombinant hyaluronidase	38:72	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom	0:90	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system.					
29455388	9	40	theme	TRU/mg	1370:1375	arg1	activity					1353:1360	a specific activity	1342:1360	a specific activity of 1097 TRU/mg	1342:1375	rTsHyal-1 partially purified through cation exchange chromatography presented a specific activity of 1097 TRU/mg, against 838 TRU/mg for the final expressed material, representing a 1.31-fold purification.					
29455388	12	41	theme	substrate	1813:1821	arg1	specificity					1823:1833	the highest substrate specificity	1801:1833	the highest substrate specificity to hyaluronan followed by chondroitin-6-sulfate, chondroitin-4-sulfate, and dermatan sulfate	1801:1926	rTsHyal-1 presents the highest substrate specificity to hyaluronan followed by chondroitin-6-sulfate, chondroitin-4-sulfate, and dermatan sulfate and showed an optimum activity at pH 6.0 and 40 °C.					
29455388	11	42	theme	Orbitrap	1721:1728	arg1	MS					1730:1731	MALDI-TOF and Q Exactive Orbitrap MS	1696:1731	MS	1730:1731	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	4	43	theme	hyaluronidase	597:609	arg1	expression					583:592	heterologous expression	570:592	heterologous expression of hyaluronidase	570:609	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	4	43	theme	hyaluronidase	597:609	arg1	tool					638:641	a biotechnological tool	619:641	a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies	619:729	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	11	44	theme	Q	1710:1710	arg1	MS					1730:1731	MALDI-TOF and Q Exactive Orbitrap MS	1696:1731	MS	1730:1731	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	5	45	theme	Pichia	862:867	arg1	pastoris					869:876	the Pichia pastoris	858:876	the Pichia pastoris system	858:883	Therefore, this study produced a recombinant hyaluronidase from Tityus serrulatus scorpion venom, designated as rTsHyal-1, in the Pichia pastoris system.					
29455388	5	46	theme	Tityus	796:801	arg1	venom					823:827	Tityus serrulatus scorpion venom	796:827	Tityus serrulatus scorpion venom	796:827	Therefore, this study produced a recombinant hyaluronidase from Tityus serrulatus scorpion venom, designated as rTsHyal-1, in the Pichia pastoris system.					
29455388	11	47	theme	de	1650:1651	arg1	sequencing					1658:1667	de novo sequencing	1650:1667	de novo sequencing	1650:1667	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	7	48	theme	minimal	1107:1113	arg1	medium					1115:1120	a buffered minimal medium	1096:1120	a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol	1096:1187	rTsHyal-1 was expressed in laboratorial scale in a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol.					
29455388	3	49	from	venoms	451:456	arg1	hyaluronidase					425:437	hyaluronidase	425:437	hyaluronidase from animal venoms	425:456	However, the in-depth characterization of hyaluronidase from animal venoms has been neglected due to its instability and low concentration in the venom, which hamper its isolation.					
29455388	3	49	from	venoms	451:456	arg1	characterization					405:420	the in-depth characterization	392:420	the in-depth characterization of hyaluronidase from animal venoms	392:456	However, the in-depth characterization of hyaluronidase from animal venoms has been neglected due to its instability and low concentration in the venom, which hamper its isolation.					
29455388	3	50	theme	low	504:506	arg1	concentration					508:520	low concentration	504:520	low concentration	504:520	However, the in-depth characterization of hyaluronidase from animal venoms has been neglected due to its instability and low concentration in the venom, which hamper its isolation.					
29455388	7	51	contain	containing	1122:1131	arg2	BMM					1143:1145	BMM	1143:1145	BMM	1143:1145	rTsHyal-1 was expressed in laboratorial scale in a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol.					
29455388	7	51	contain	containing	1122:1131	arg2	methanol					1133:1140	methanol	1133:1140	methanol (BMM) for 96 h with daily addition of methanol	1133:1187	rTsHyal-1 was expressed in laboratorial scale in a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol.					
29455388	7	51	contain	containing	1122:1131	arg1	medium					1115:1120	a buffered minimal medium	1096:1120	a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol	1096:1187	rTsHyal-1 was expressed in laboratorial scale in a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol.					
29455388	9	52	theme	expressed	1411:1419	arg1	material					1421:1428	the final expressed material	1401:1428	the final expressed material	1401:1428	rTsHyal-1 partially purified through cation exchange chromatography presented a specific activity of 1097 TRU/mg, against 838 TRU/mg for the final expressed material, representing a 1.31-fold purification.					
29455388	10	53	with	analysis	1544:1551	arg1	F					1538:1538	PNGase F	1531:1538	PNGase F	1531:1538	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	10	54	theme	mass	1556:1559	arg1	MALDI-TOF					1575:1583	MALDI-TOF	1575:1583	MALDI-TOF	1575:1583	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	10	54	theme	mass	1556:1559	arg1	spectrometry					1561:1572	mass spectrometry	1556:1572	mass spectrometry (MALDI-TOF)	1556:1584	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	11	55	theme	rTsHyal-1	1672:1680	arg1	sequencing					1658:1667	de novo sequencing	1650:1667	de novo sequencing	1650:1667	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	8	56	theme	total	1228:1232	arg1	yield					1242:1246	a total protein yield	1226:1246	a total protein yield of 0.266 mg/mL	1226:1261	Expression of rTsHyal-1 resulted in a total protein yield of 0.266 mg/mL.					
29455388	1	57	theme	broad	162:166	arg1	application					178:188	a broad potential application	160:188	a broad potential application	160:188	In general, hyaluronidases have a broad potential application on medicine and esthetics fields.					
29455388	14	58	from	venoms	2136:2141	arg1	hyaluronidase					2108:2120	the first recombinant hyaluronidase	2086:2120	the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity	2086:2208	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	14	58	from	venoms	2136:2141	arg1	This					2078:2081	This	2078:2081	This	2078:2081	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	5	59	theme	recombinant	765:775	arg1	hyaluronidase					777:789	a recombinant hyaluronidase	763:789	a recombinant hyaluronidase	763:789	Therefore, this study produced a recombinant hyaluronidase from Tityus serrulatus scorpion venom, designated as rTsHyal-1, in the Pichia pastoris system.					
29455388	6	60	from	expression	1020:1029	arg1	pastoris					1037:1044	P. pastoris	1034:1044	P. pastoris	1034:1044	|Thus, a gene for TsHyal-1 (gb||KF623285.1) was synthesized and cloned into the pPICZαA vector (GenScript Corporation) for heterologous expression in P. pastoris.|					
29455388	2	61	theme	spreading	323:331	arg1	factors					333:339	spreading factors	323:339	spreading factors of toxins	323:349	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	5	62	theme	pastoris	869:876	arg1	system					878:883	the Pichia pastoris system	858:883	the Pichia pastoris system	858:883	Therefore, this study produced a recombinant hyaluronidase from Tityus serrulatus scorpion venom, designated as rTsHyal-1, in the Pichia pastoris system.					
29455388	5	63	from	hyaluronidase	777:789	arg1	system					878:883	the Pichia pastoris system	858:883	the Pichia pastoris system	858:883	Therefore, this study produced a recombinant hyaluronidase from Tityus serrulatus scorpion venom, designated as rTsHyal-1, in the Pichia pastoris system.					
29455388	8	64	theme	protein	1234:1240	arg1	yield					1242:1246	a total protein yield	1226:1246	a total protein yield of 0.266 mg/mL	1226:1261	Expression of rTsHyal-1 resulted in a total protein yield of 0.266 mg/mL.					
29455388	10	65	theme	4.5 kDa	1627:1633	arg1	N-glycosylation					1608:1622	a potential N-glycosylation	1596:1622	a potential N-glycosylation of 4.5 kDa	1596:1633	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	11	66	theme	coverage	1772:1779	arg1	%					1750:1750	46.8%	1746:1750	46.8% of protein sequence coverage	1746:1779	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	11	66	theme	coverage	1772:1779	arg1	coverage					1772:1779	protein sequence coverage	1755:1779	protein sequence coverage	1755:1779	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	2	67	theme	animal	244:249	arg1	venoms					251:256	animal venoms	244:256	animal venoms	244:256	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	1	68	theme	esthetics	206:214	arg1	fields					216:221	esthetics fields	206:221	esthetics fields	206:221	In general, hyaluronidases have a broad potential application on medicine and esthetics fields.					
29455388	4	69	theme	structural	697:706	arg1	studies					723:729	structural and functional studies	697:729	structural and functional studies	697:729	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	2	70	theme	present	276:282	arg1	hyaluronan					265:274	hyaluronan	265:274	hyaluronan	265:274	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	6	71	theme	heterologous	1007:1018	arg1	expression					1020:1029	heterologous expression	1007:1029	heterologous expression in P. pastoris	1007:1044	|Thus, a gene for TsHyal-1 (gb||KF623285.1) was synthesized and cloned into the pPICZαA vector (GenScript Corporation) for heterologous expression in P. pastoris.|					
29455388	4	72	theme	functional	712:721	arg1	studies					723:729	structural and functional studies	697:729	structural and functional studies	697:729	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	14	73	theme	preserved	2184:2192	arg1	activity					2201:2208	preserved enzyme activity	2184:2208	preserved enzyme activity	2184:2208	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	0	74	theme	venom	86:90	arg1	hyaluronidase					60:72	the first recombinant hyaluronidase	38:72	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom	0:90	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system.					
29455388	4	75	theme	enzyme	686:691	arg1	enzyme					686:691	the enzyme	682:691	the enzyme	682:691	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	4	75	theme	enzyme	686:691	arg1	amounts					671:677	enough amounts	664:677	enough amounts of the enzyme	664:691	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	11	76	theme	protein	1755:1761	arg1	coverage					1772:1779	protein sequence coverage	1755:1779	protein sequence coverage	1755:1779	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	12	77	theme	optimum	1942:1948	arg1	activity					1950:1957	an optimum activity	1939:1957	an optimum activity	1939:1957	rTsHyal-1 presents the highest substrate specificity to hyaluronan followed by chondroitin-6-sulfate, chondroitin-4-sulfate, and dermatan sulfate and showed an optimum activity at pH 6.0 and 40 °C.					
29455388	10	78	with	treatment	1516:1524	arg1	F					1538:1538	PNGase F	1531:1538	PNGase F	1531:1538	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	0	79	theme	pastoris	111:118	arg1	system					120:125	Pichia pastoris system	104:125	Pichia pastoris system	104:125	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system.					
29455388	9	80	theme	cation	1301:1306	arg1	chromatography					1317:1330	cation exchange chromatography	1301:1330	cation exchange chromatography	1301:1330	rTsHyal-1 partially purified through cation exchange chromatography presented a specific activity of 1097 TRU/mg, against 838 TRU/mg for the final expressed material, representing a 1.31-fold purification.					
29455388	4	81	theme	biotechnological	621:636	arg1	expression					583:592	heterologous expression	570:592	heterologous expression of hyaluronidase	570:609	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	4	81	theme	biotechnological	621:636	arg1	tool					638:641	a biotechnological tool	619:641	a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies	619:729	Thus, heterologous expression of hyaluronidase acts as a biotechnological tool in the obtainment of enough amounts of the enzyme for structural and functional studies.					
29455388	14	82	theme	scorpion	2127:2134	arg1	venoms					2136:2141	scorpion venoms	2127:2141	scorpion venoms expressed in the P. pastoris system with preserved enzyme activity	2127:2208	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	7	83	theme	daily	1162:1166	arg1	addition					1168:1175	daily addition	1162:1175	daily addition of methanol	1162:1187	rTsHyal-1 was expressed in laboratorial scale in a buffered minimal medium containing methanol (BMM) for 96 h with daily addition of methanol.					
29455388	5	84	theme	scorpion	814:821	arg1	venom					823:827	Tityus serrulatus scorpion venom	796:827	Tityus serrulatus scorpion venom	796:827	Therefore, this study produced a recombinant hyaluronidase from Tityus serrulatus scorpion venom, designated as rTsHyal-1, in the Pichia pastoris system.					
29455388	5	85	from	venom	823:827	arg1	hyaluronidase					777:789	a recombinant hyaluronidase	763:789	a recombinant hyaluronidase	763:789	Therefore, this study produced a recombinant hyaluronidase from Tityus serrulatus scorpion venom, designated as rTsHyal-1, in the Pichia pastoris system.					
29455388	13	86	theme	rTsHyal-1	2067:2075	arg1	expression					2053:2062	the heterologous expression	2036:2062	the heterologous expression of rTsHyal-1	2036:2075	These results validate the biotechnological process for the heterologous expression of rTsHyal-1.					
29455388	14	87	with	system	2172:2177	arg1	activity					2201:2208	preserved enzyme activity	2184:2208	preserved enzyme activity	2184:2208	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	2	88	theme	victim	375:380	arg1	tissues					360:366	the tissues	356:366	the tissues of the victim	356:380	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	0	89	theme	rTsHyal-1	27:35	arg1	expression					13:22	Heterologous expression	0:22	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom	0:90	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system.					
29455388	2	90	from	venoms	251:256	arg1	Hyaluronidases					224:237	Hyaluronidases	224:237	Hyaluronidases from animal venoms	224:256	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	14	91	theme	first	2090:2094	arg1	hyaluronidase					2108:2120	the first recombinant hyaluronidase	2086:2120	the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity	2086:2208	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	14	91	theme	first	2090:2094	arg1	This					2078:2081	This	2078:2081	This	2078:2081	This is the first recombinant hyaluronidase from scorpion venoms expressed in the P. pastoris system with preserved enzyme activity.					
29455388	11	92	dep	de	1650:1651	arg1	novo					1653:1656	novo	1653:1656	novo	1653:1656	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	10	93	theme	molecular	1484:1492	arg1	mass					1494:1497	molecular mass	1484:1497	molecular mass of 49.5 kDa	1484:1509	rTsHyal-1 has molecular mass of 49.5 kDa, and treatment with PNGase F and analysis by mass spectrometry (MALDI-TOF) indicated a potential N-glycosylation of 4.5 kDa.					
29455388	0	94	theme	recombinant	48:58	arg1	hyaluronidase					60:72	the first recombinant hyaluronidase	38:72	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom	0:90	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system.					
29455388	3	95	theme	in-depth	396:403	arg1	characterization					405:420	the in-depth characterization	392:420	the in-depth characterization of hyaluronidase from animal venoms	392:456	However, the in-depth characterization of hyaluronidase from animal venoms has been neglected due to its instability and low concentration in the venom, which hamper its isolation.					
29455388	11	96	theme	Exactive	1712:1719	arg1	MS					1730:1731	MALDI-TOF and Q Exactive Orbitrap MS	1696:1731	MS	1730:1731	Additionally, de novo sequencing of rTsHyal-1, performed in MALDI-TOF and Q Exactive Orbitrap MS, resulted in 46.8% of protein sequence coverage.					
29455388	13	97	theme	heterologous	2040:2051	arg1	expression					2053:2062	the heterologous expression	2036:2062	the heterologous expression of rTsHyal-1	2036:2075	These results validate the biotechnological process for the heterologous expression of rTsHyal-1.					
29455388	2	98	theme	toxins	344:349	arg1	factors					333:339	spreading factors	323:339	spreading factors of toxins	323:349	Hyaluronidases from animal venoms cleave hyaluronan present in the extracellular matrix, acting as spreading factors of toxins into the tissues of the victim.					
29455388	12	99	theme	highest	1805:1811	arg1	specificity					1823:1833	the highest substrate specificity	1801:1833	the highest substrate specificity to hyaluronan followed by chondroitin-6-sulfate, chondroitin-4-sulfate, and dermatan sulfate	1801:1926	rTsHyal-1 presents the highest substrate specificity to hyaluronan followed by chondroitin-6-sulfate, chondroitin-4-sulfate, and dermatan sulfate and showed an optimum activity at pH 6.0 and 40 °C.					
29455388	0	100	dep	expression	13:22	arg1	hyaluronidase					60:72	the first recombinant hyaluronidase	38:72	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom	0:90	Heterologous expression of rTsHyal-1: the first recombinant hyaluronidase of scorpion venom produced in Pichia pastoris system.					
29455388	13	101	theme	biotechnological	2007:2022	arg1	process					2024:2030	the biotechnological process	2003:2030	the biotechnological process for the heterologous expression of rTsHyal-1	2003:2075	These results validate the biotechnological process for the heterologous expression of rTsHyal-1.					
31320049	5	0	theme	release	779:785	arg1	profiles					787:794	The release profiles	775:794	The release profiles yielded from the measurements of cumulative drug delivery	775:852	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	1	1	theme	release	139:145	arg1	attributes					147:156	The release attributes	135:156	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	135:236	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	3	2	from	traits	557:562	arg1	conditions					620:629	various physiological pH conditions	595:629	various physiological pH conditions	595:629	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	5	3	with	system	870:875	arg1	ESR					888:890	lower ESR	882:890	lower ESR	882:890	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	0	4	theme	5-Fluorouracil	66:79	arg1	encapsulation					81:93	5-Fluorouracil encapsulation	66:93	5-Fluorouracil encapsulation	66:93	Gum ghatti based hydrogel: Microwave synthesis, characterization, 5-Fluorouracil encapsulation and 'in vitro' drug release evaluation.					
31320049	5	5	theme	release	908:914	arg1	rate					916:919	slower release rate	901:919	slower release rate than that of the system	901:943	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	0	6	dep	in	100:101	arg1	vitro					103:107	vitro	103:107	vitro	103:107	Gum ghatti based hydrogel: Microwave synthesis, characterization, 5-Fluorouracil encapsulation and 'in vitro' drug release evaluation.					
31320049	3	7	theme	synthesized	495:505	arg1	system					507:512	The synthesized system	491:512	The synthesized system	491:512	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	2	8	theme	mesh	415:418	arg1	size					420:423	mesh size	415:423	mesh size	415:423	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	3	9	theme	physicochemical	541:555	arg1	traits					557:562	physicochemical traits	541:562	physicochemical traits	541:562	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	4	10	theme	crosslinking	669:680	arg1	degree					659:664	The degree	655:664	The degree of crosslinking analysed through equilibrium swelling ratio (ESR)	655:730	The degree of crosslinking analysed through equilibrium swelling ratio (ESR) has been correlated with release kinetics.					
31320049	0	11	theme	in	100:101	arg1	evaluation					123:132	'in vitro' drug release evaluation	99:132	'in vitro' drug release evaluation	99:132	Gum ghatti based hydrogel: Microwave synthesis, characterization, 5-Fluorouracil encapsulation and 'in vitro' drug release evaluation.					
31320049	5	12	theme	cumulative	829:838	arg1	delivery					845:852	cumulative drug delivery	829:852	cumulative drug delivery	829:852	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	1	13	theme	anticancer	161:170	arg1	matrices					229:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	2	14	theme	gum	452:454	arg1	copolymerization					432:447	copolymerization	432:447	copolymerization of gum	432:454	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	3	15	theme	drug	579:582	arg1	release					584:590	'in-vitro' drug release	568:590	'in-vitro' drug release in various physiological pH conditions by USP standard method	568:652	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	1	16	theme	therapeutic	172:182	arg1	matrices					229:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	0	17	theme	Gum	0:2	arg1	ghatti					4:9	Gum ghatti	0:9	Gum ghatti	0:9	Gum ghatti based hydrogel: Microwave synthesis, characterization, 5-Fluorouracil encapsulation and 'in vitro' drug release evaluation.					
31320049	2	18	theme	product	311:317	arg1	composition					319:329	the product composition	307:329	the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm))	307:402	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	1	19	theme	embedded	184:191	arg1	matrices					229:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	5	20	theme	drug	840:843	arg1	delivery					845:852	cumulative drug delivery	829:852	cumulative drug delivery	829:852	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	0	21	theme	drug	110:113	arg1	evaluation					123:132	'in vitro' drug release evaluation	99:132	'in vitro' drug release evaluation	99:132	Gum ghatti based hydrogel: Microwave synthesis, characterization, 5-Fluorouracil encapsulation and 'in vitro' drug release evaluation.					
31320049	3	22	theme	in-vitro	569:576	arg1	release					584:590	'in-vitro' drug release	568:590	'in-vitro' drug release in various physiological pH conditions by USP standard method	568:652	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	2	23	dep	composition	319:329	arg1	N					368:368	monomer-acrylamide and crosslinker- N	332:368	monomer-acrylamide and crosslinker- N	332:368	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	2	23	dep	composition	319:329	arg1	Methylenebisacrylamide					373:394	N Methylenebisacrylamide	371:394	N Methylenebisacrylamide (MBAm)	371:401	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	6	24	theme	pH	1054:1055	arg1	milieu					1057:1062	pH milieu	1054:1062	pH milieu	1054:1062	Additionally, the release rate rises with rise in pH milieu.					
31320049	5	25	theme	delivery	845:852	arg1	measurements					813:824	the measurements	809:824	the measurements of cumulative drug delivery	809:852	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	5	26	theme	higher	950:955	arg1	ESR					957:959	higher ESR	950:959	higher ESR	950:959	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	4	27	theme	release	757:763	arg1	kinetics					765:772	release kinetics	757:772	release kinetics	757:772	The degree of crosslinking analysed through equilibrium swelling ratio (ESR) has been correlated with release kinetics.					
31320049	1	28	theme	programmable	196:207	arg1	gum					209:211	programmable gum	196:211	programmable gum	196:211	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	0	29	theme	Microwave	27:35	arg1	synthesis					37:45	Microwave synthesis	27:45	Microwave synthesis	27:45	Gum ghatti based hydrogel: Microwave synthesis, characterization, 5-Fluorouracil encapsulation and 'in vitro' drug release evaluation.					
31320049	3	30	theme	physiological	603:615	arg1	conditions					620:629	various physiological pH conditions	595:629	various physiological pH conditions	595:629	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	2	31	theme	based	474:478	arg1	technique					480:488	microwave based technique	464:488	microwave based technique	464:488	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	5	32	theme	lower	882:886	arg1	ESR					888:890	lower ESR	882:890	lower ESR	882:890	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	0	33	theme	release	115:121	arg1	evaluation					123:132	'in vitro' drug release evaluation	99:132	'in vitro' drug release evaluation	99:132	Gum ghatti based hydrogel: Microwave synthesis, characterization, 5-Fluorouracil encapsulation and 'in vitro' drug release evaluation.					
31320049	3	34	theme	pH	617:618	arg1	conditions					620:629	various physiological pH conditions	595:629	various physiological pH conditions	595:629	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	1	35	from	gum	209:211	arg1	embedded					184:191	embedded	184:191	embedded	184:191	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	1	36	theme	ghatti	213:218	arg1	matrices					229:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	2	37	theme	microwave	464:472	arg1	technique					480:488	microwave based technique	464:488	microwave based technique	464:488	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	5	38	theme	slower	901:906	arg1	rate					916:919	slower release rate	901:919	slower release rate than that of the system	901:943	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	2	39	theme	crosslinker-	355:366	arg1	N					368:368	monomer-acrylamide and crosslinker- N	332:368	monomer-acrylamide and crosslinker- N	332:368	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	2	39	theme	crosslinker-	355:366	arg1	Methylenebisacrylamide					373:394	N Methylenebisacrylamide	371:394	N Methylenebisacrylamide (MBAm)	371:401	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	3	40	theme	various	595:601	arg1	conditions					620:629	various physiological pH conditions	595:629	various physiological pH conditions	595:629	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	5	41	dep	higher	985:990	arg1	values					996:1001	t50 values	992:1001	higher t50 values	985:1001	The release profiles yielded from the measurements of cumulative drug delivery showed that the system with lower ESR included slower release rate than that of the system with higher ESR, consequently generated higher t50 values.					
31320049	3	42	theme	USP	634:636	arg1	method					647:652	USP standard method	634:652	USP standard method	634:652	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	2	43	theme	monomer-acrylamide	332:349	arg1	N					368:368	monomer-acrylamide and crosslinker- N	332:368	monomer-acrylamide and crosslinker- N	332:368	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	2	43	theme	monomer-acrylamide	332:349	arg1	Methylenebisacrylamide					373:394	N Methylenebisacrylamide	371:394	N Methylenebisacrylamide (MBAm)	371:401	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	1	44	theme	hydrogel	220:227	arg1	matrices					229:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	161:236	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	3	45	theme	standard	638:645	arg1	method					647:652	USP standard method	634:652	USP standard method	634:652	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	4	46	theme	equilibrium	699:709	arg1	ratio					720:724	equilibrium swelling ratio	699:724	equilibrium swelling ratio (ESR)	699:730	The degree of crosslinking analysed through equilibrium swelling ratio (ESR) has been correlated with release kinetics.					
31320049	4	46	theme	equilibrium	699:709	arg1	ESR					727:729	ESR	727:729	ESR	727:729	The degree of crosslinking analysed through equilibrium swelling ratio (ESR) has been correlated with release kinetics.					
31320049	1	47	theme	matrices	229:236	arg1	attributes					147:156	The release attributes	135:156	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices	135:236	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	1	48	from	embedded	184:191	arg1	gum					209:211	programmable gum	196:211	programmable gum	196:211	The release attributes of anticancer therapeutic embedded in programmable gum ghatti hydrogel matrices has been evaluated.					
31320049	4	49	theme	swelling	711:718	arg1	ratio					720:724	equilibrium swelling ratio	699:724	equilibrium swelling ratio (ESR)	699:730	The degree of crosslinking analysed through equilibrium swelling ratio (ESR) has been correlated with release kinetics.					
31320049	4	49	theme	swelling	711:718	arg1	ESR					727:729	ESR	727:729	ESR	727:729	The degree of crosslinking analysed through equilibrium swelling ratio (ESR) has been correlated with release kinetics.					
31320049	3	50	from	release	584:590	arg1	conditions					620:629	various physiological pH conditions	595:629	various physiological pH conditions	595:629	The synthesized system has been characterized for physicochemical traits and 'in-vitro' drug release in various physiological pH conditions by USP standard method.					
31320049	6	51	theme	release	1022:1028	arg1	rate					1030:1033	the release rate	1018:1033	the release rate	1018:1033	Additionally, the release rate rises with rise in pH milieu.					
31320049	2	52	theme	N	371:371	arg1	MBAm					397:400	MBAm	397:400	MBAm	397:400	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	2	52	theme	N	371:371	arg1	N					368:368	monomer-acrylamide and crosslinker- N	332:368	monomer-acrylamide and crosslinker- N	332:368	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
31320049	2	52	theme	N	371:371	arg1	Methylenebisacrylamide					373:394	N Methylenebisacrylamide	371:394	N Methylenebisacrylamide (MBAm)	371:401	Hydrogel has been programmed simply by adjusting the product composition (monomer-acrylamide and crosslinker- N, N Methylenebisacrylamide (MBAm)) to control mesh size during copolymerization of gum through microwave based technique.					
30827632	0	0	theme	structural	73:82	arg1	properties					84:93	the structural properties	69:93	the structural properties of lotus seed resistant starch	69:124	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	6	1	theme	hydrated	933:940	arg1	chains					949:954	the hydrated starch chains	929:954	the hydrated starch chains	929:954	Bound water was related to the hydrated starch chains, while channel water in B-type crystal represented the micro-crystalline structure.					
30827632	4	2	theme	resistant	614:622	arg1	starches					624:631	resistant starches	614:631	resistant starches prepared with 70% and 80% water content	614:671	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	3	3	theme	other	477:481	arg1	samples					483:489	other samples	477:489	other samples	477:489	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	7	4	used	used	1095:1098	arg2	model					1073:1077	moisture distribution model	1051:1077	moisture distribution model	1051:1077	Therefore, moisture distribution model can be feasibly used to depict the structural properties of resistant starch.					
30827632	7	5	theme	distribution	1060:1071	arg1	model					1073:1077	moisture distribution model	1051:1077	moisture distribution model	1051:1077	Therefore, moisture distribution model can be feasibly used to depict the structural properties of resistant starch.					
30827632	5	6	from	properties	824:833	arg1	them					885:888	them	885:888	them	885:888	Two corresponding relationships between the structural properties of resistant starch and the water distribution in them were found.					
30827632	1	7	theme	structural	237:246	arg1	properties					248:257	the structural properties	233:257	the structural properties of lotus seed resistant starch	233:288	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	6	8	theme	micro-crystalline	1011:1027	arg1	structure					1029:1037	the micro-crystalline structure	1007:1037	the micro-crystalline structure	1007:1037	Bound water was related to the hydrated starch chains, while channel water in B-type crystal represented the micro-crystalline structure.					
30827632	7	9	theme	starch	1149:1154	arg1	properties					1125:1134	the structural properties	1110:1134	the structural properties of resistant starch	1110:1154	Therefore, moisture distribution model can be feasibly used to depict the structural properties of resistant starch.					
30827632	7	10	theme	resistant	1139:1147	arg1	starch					1149:1154	resistant starch	1139:1154	resistant starch	1139:1154	Therefore, moisture distribution model can be feasibly used to depict the structural properties of resistant starch.					
30827632	4	11	theme	water	659:663	arg1	content					665:671	80% water content	655:671	80% water content	655:671	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	0	12	theme	seed	104:107	arg1	starch					119:124	lotus seed resistant starch	98:124	lotus seed resistant starch	98:124	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	6	13	theme	channel	963:969	arg1	water					971:975	channel water	963:975	channel water in B-type crystal	963:993	Bound water was related to the hydrated starch chains, while channel water in B-type crystal represented the micro-crystalline structure.					
30827632	3	14	theme	flake	503:507	arg1	structure					509:517	a flake structure	501:517	a flake structure	501:517	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	6	15	theme	B-type	980:985	arg1	crystal					987:993	B-type crystal	980:993	B-type crystal	980:993	Bound water was related to the hydrated starch chains, while channel water in B-type crystal represented the micro-crystalline structure.					
30827632	0	16	theme	lotus	98:102	arg1	starch					119:124	lotus seed resistant starch	98:124	lotus seed resistant starch	98:124	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	0	17	from	effect	42:47	arg1	properties					84:93	the structural properties	69:93	the structural properties of lotus seed resistant starch	69:124	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	3	18	theme	compact	378:384	arg1	structure					386:394	The compact structure	374:394	The compact structure	374:394	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	1	19	theme	moisture	158:165	arg1	model					180:184	a moisture distribution model	156:184	a moisture distribution model	156:184	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	0	20	theme	distribution	9:20	arg1	model					22:26	Moisture distribution model	0:26	Moisture distribution model	0:26	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	6	21	theme	Bound	902:906	arg1	water					908:912	Bound water	902:912	Bound water	902:912	Bound water was related to the hydrated starch chains, while channel water in B-type crystal represented the micro-crystalline structure.					
30827632	5	22	theme	water	863:867	arg1	distribution					869:880	the water distribution	859:880	the water distribution in them	859:888	Two corresponding relationships between the structural properties of resistant starch and the water distribution in them were found.					
30827632	1	23	theme	distribution	167:178	arg1	model					180:184	a moisture distribution model	156:184	a moisture distribution model	156:184	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	0	24	theme	Moisture	0:7	arg1	model					22:26	Moisture distribution model	0:26	Moisture distribution model	0:26	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	1	25	theme	lotus	262:266	arg1	starch					283:288	lotus seed resistant starch	262:288	lotus seed resistant starch	262:288	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	6	26	from	water	971:975	arg1	crystal					987:993	B-type crystal	980:993	B-type crystal	980:993	Bound water was related to the hydrated starch chains, while channel water in B-type crystal represented the micro-crystalline structure.					
30827632	5	27	theme	corresponding	773:785	arg1	relationships					787:799	Two corresponding relationships	769:799	Two corresponding relationships between the structural properties of resistant starch and the water distribution in them	769:888	Two corresponding relationships between the structural properties of resistant starch and the water distribution in them were found.					
30827632	2	28	theme	starch	366:371	arg1	composition					341:351	the composition	337:351	the composition of resistant starch	337:371	The result showed that water content affected the composition of resistant starch.					
30827632	1	29	theme	seed	268:271	arg1	starch					283:288	lotus seed resistant starch	262:288	lotus seed resistant starch	262:288	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	0	30	theme	starch	119:124	arg1	properties					84:93	the structural properties	69:93	the structural properties of lotus seed resistant starch	69:124	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	4	31	theme	%	657:657	arg1	content					665:671	80% water content	655:671	80% water content	655:671	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	4	32	theme	excess	586:591	arg1	water					593:597	excess water	586:597	excess water (95% and 90%)	586:611	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	1	33	theme	resistant	273:281	arg1	starch					283:288	lotus seed resistant starch	262:288	lotus seed resistant starch	262:288	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	0	34	theme	resistant	109:117	arg1	starch					119:124	lotus seed resistant starch	98:124	lotus seed resistant starch	98:124	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	4	35	theme	80	655:656	arg1	%					657:657	%	657:657	%	657:657	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	3	36	theme	50	458:459	arg1	%					460:460	%	460:460	%	460:460	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	1	37	theme	starch	283:288	arg1	properties					248:257	the structural properties	233:257	the structural properties of lotus seed resistant starch	233:288	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	3	38	located	observed	400:407	arg1	surface					416:422	the surface	412:422	the surface of resistant starch prepared with 50% water content	412:474	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	3	38	located	observed	400:407	arg2	structure					386:394	The compact structure	374:394	The compact structure	374:394	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	5	39	from	distribution	869:880	arg1	them					885:888	them	885:888	them	885:888	Two corresponding relationships between the structural properties of resistant starch and the water distribution in them were found.					
30827632	3	40	theme	starch	437:442	arg1	surface					416:422	the surface	412:422	the surface of resistant starch prepared with 50% water content	412:474	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	7	41	theme	moisture	1051:1058	arg1	model					1073:1077	moisture distribution model	1051:1077	moisture distribution model	1051:1077	Therefore, moisture distribution model can be feasibly used to depict the structural properties of resistant starch.					
30827632	4	42	dep	water	593:597	arg1	%					602:602	95%	600:602	95%	600:602	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	4	42	dep	water	593:597	arg1	%					610:610	90%	608:610	90%	608:610	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	0	43	theme	water	52:56	arg1	content					58:64	water content	52:64	water content	52:64	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	4	44	dep	insufficient	556:567	arg1	%					572:572	50%	570:572	50%	570:572	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	4	44	dep	insufficient	556:567	arg1	%					580:580	60%	578:580	60%	578:580	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	4	45	theme	double	743:748	arg1	helix					750:754	more double helix	738:754	more double helix	738:754	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	1	46	from	influence	203:211	arg1	properties					248:257	the structural properties	233:257	the structural properties of lotus seed resistant starch	233:288	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	5	47	theme	resistant	838:846	arg1	starch					848:853	resistant starch	838:853	resistant starch	838:853	Two corresponding relationships between the structural properties of resistant starch and the water distribution in them were found.					
30827632	3	48	theme	%	460:460	arg1	content					468:474	50% water content	458:474	50% water content	458:474	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	4	49	theme	more	738:741	arg1	helix					750:754	more double helix	738:754	more double helix	738:754	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	5	50	theme	starch	848:853	arg1	properties					824:833	the structural properties	809:833	the structural properties of resistant starch	809:853	Two corresponding relationships between the structural properties of resistant starch and the water distribution in them were found.					
30827632	5	50	theme	starch	848:853	arg1	distribution					869:880	the water distribution	859:880	the water distribution in them	859:888	Two corresponding relationships between the structural properties of resistant starch and the water distribution in them were found.					
30827632	4	51	theme	higher-molecular-weight	690:712	arg1	chains					714:719	higher-molecular-weight chains	690:719	higher-molecular-weight chains	690:719	Compared with samples prepared with insufficient (50% and 60%) or excess water (95% and 90%), resistant starches prepared with 70% and 80% water content were composed of higher-molecular-weight chains and consisted of more double helix and crystal.					
30827632	6	52	theme	starch	942:947	arg1	chains					949:954	the hydrated starch chains	929:954	the hydrated starch chains	929:954	Bound water was related to the hydrated starch chains, while channel water in B-type crystal represented the micro-crystalline structure.					
30827632	2	53	theme	water	314:318	arg1	content					320:326	water content	314:326	water content	314:326	The result showed that water content affected the composition of resistant starch.					
30827632	3	54	theme	water	462:466	arg1	content					468:474	50% water content	458:474	50% water content	458:474	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	1	55	theme	water	216:220	arg1	content					222:228	water content	216:228	water content	216:228	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	2	56	theme	resistant	356:364	arg1	starch					366:371	resistant starch	356:371	resistant starch	356:371	The result showed that water content affected the composition of resistant starch.					
30827632	3	57	theme	resistant	427:435	arg1	starch					437:442	resistant starch	427:442	resistant starch prepared with 50% water content	427:474	The compact structure was observed on the surface of resistant starch prepared with 50% water content; other samples displayed a flake structure.					
30827632	1	58	theme	content	222:228	arg1	influence					203:211	the influence	199:211	the influence of water content on the structural properties of lotus seed resistant starch	199:288	The objective was to utilize a moisture distribution model to summarize the influence of water content on the structural properties of lotus seed resistant starch.					
30827632	0	59	theme	content	58:64	arg1	effect					42:47	the effect	38:47	the effect of water content on the structural properties of lotus seed resistant starch	38:124	Moisture distribution model describes the effect of water content on the structural properties of lotus seed resistant starch.					
30827632	7	60	theme	structural	1114:1123	arg1	properties					1125:1134	the structural properties	1110:1134	the structural properties of resistant starch	1110:1154	Therefore, moisture distribution model can be feasibly used to depict the structural properties of resistant starch.					
30827632	5	61	theme	structural	813:822	arg1	properties					824:833	the structural properties	809:833	the structural properties of resistant starch	809:853	Two corresponding relationships between the structural properties of resistant starch and the water distribution in them were found.					
31776786	8	0	theme	rhBMP-2-loaded	1152:1165	arg1	microspheres/CS					1177:1191	rhBMP-2-loaded hollow HA microspheres/CS	1152:1191	rhBMP-2-loaded hollow HA microspheres/CS composite group	1152:1207	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	10	1	theme	inhibitor	1386:1394	arg1	group					1396:1400	the p38 inhibitor group	1378:1400	the p38 inhibitor group	1378:1400	In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group.					
31776786	8	2	theme	HA	1174:1175	arg1	microspheres/CS					1177:1191	rhBMP-2-loaded hollow HA microspheres/CS	1152:1191	rhBMP-2-loaded hollow HA microspheres/CS composite group	1152:1207	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	12	3	theme	important	1764:1772	arg1	role					1774:1777	an important role	1761:1777	an important role	1761:1777	CONCLUSION The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.					
31776786	11	4	theme	rhBMP-2	1510:1516	arg1	group					1518:1522	rhBMP-2 group	1510:1522	rhBMP-2 group	1510:1522	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	1	5	theme	signaling	301:309	arg1	pathway					311:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	1	5	theme	signaling	301:309	arg1	pathway					273:279	the non-Smad dependent TAK1 signaling pathway	235:279	the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway)	235:318	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	8	6	theme	composite	1193:1201	arg1	group					1203:1207	rhBMP-2-loaded hollow HA microspheres/CS composite group	1152:1207	rhBMP-2-loaded hollow HA microspheres/CS composite group	1152:1207	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	11	7	theme	TAK1	1654:1657	arg1	group					1669:1673	TAK1 inhibitor group	1654:1673	TAK1 inhibitor group	1654:1673	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	11	8	theme	microspheres/CS	1552:1566	arg1	group					1578:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	1	9	theme	present	195:201	arg1	study					203:207	The present study	191:207	The present study	191:207	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	11	10	theme	hollow	1542:1547	arg1	group					1578:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	3	11	theme	calcium	602:608	arg1	deposition					610:619	calcium deposition	602:619	calcium deposition	602:619	Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed.					
31776786	5	12	theme	bioactive	889:897	arg1	form					899:902	bioactive form	889:902	bioactive form	889:902	RESULTS RhBMP-2 could be loaded and released from the complex in bioactive form.					
31776786	6	13	theme	HA/CS	981:985	arg1	scaffolds					987:995	HA/CS scaffolds	981:995	HA/CS scaffolds	981:995	Additionally, the complex provided a prolonged period of time compared with HA/CS scaffolds.					
31776786	4	14	theme	markers	744:750	arg1	expression					700:709	the mRNAs expression	690:709	the mRNAs expression of TAK1, p38, Osx and osteogenic markers	690:750	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	9	15	theme	load	1243:1246	arg1	values					1248:1253	load values	1243:1253	load values in p38 inhibitor group	1243:1276	In the three-point bending test, load values in p38 inhibitor group decreased.					
31776786	7	16	theme	p38	1077:1079	arg1	group					1091:1095	p38 inhibitor group	1077:1095	p38 inhibitor group	1077:1095	Serum ALP activity was significantly decreased in the TAK1 inhibitor group and p38 inhibitor group.					
31776786	4	17	theme	Osx	725:727	arg1	expression					700:709	the mRNAs expression	690:709	the mRNAs expression of TAK1, p38, Osx and osteogenic markers	690:750	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	0	18	theme	microspheres/chitosan	153:173	arg1	composite					175:183	hollow hydroxyapatite microspheres/chitosan composite	131:183	hollow hydroxyapatite microspheres/chitosan composite	131:183	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	9	19	theme	inhibitor	1262:1270	arg1	group					1272:1276	p38 inhibitor group	1258:1276	p38 inhibitor group	1258:1276	In the three-point bending test, load values in p38 inhibitor group decreased.					
31776786	6	20	theme	prolonged	942:950	arg1	period					952:957	a prolonged period	940:957	a prolonged period of time	940:965	Additionally, the complex provided a prolonged period of time compared with HA/CS scaffolds.					
31776786	11	21	theme	rhBMP-2-loaded	1527:1540	arg1	group					1578:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	4	22	theme	osteogenic	733:742	arg1	markers					744:750	osteogenic markers	733:750	osteogenic markers	733:750	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	7	23	theme	TAK1	1052:1055	arg1	group					1067:1071	the TAK1 inhibitor group	1048:1071	the TAK1 inhibitor group	1048:1071	Serum ALP activity was significantly decreased in the TAK1 inhibitor group and p38 inhibitor group.					
31776786	7	24	theme	ALP	1004:1006	arg1	activity					1008:1015	Serum ALP activity	998:1015	Serum ALP activity	998:1015	Serum ALP activity was significantly decreased in the TAK1 inhibitor group and p38 inhibitor group.					
31776786	10	25	theme	mRNA	1314:1317	arg1	expression					1319:1328	the mRNA expression	1310:1328	the mRNA expression of BSP on day 90	1310:1345	In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group.					
31776786	12	26	theme	signaling	1734:1742	arg1	pathway					1744:1750	The BMP-2-TAK1-p38-OSX signaling pathway	1711:1750	The BMP-2-TAK1-p38-OSX signaling pathway	1711:1750	CONCLUSION The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.					
31776786	10	27	from	expression	1319:1328	arg1	day					1340:1342	day 90	1340:1345	day 90	1340:1345	In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group.					
31776786	4	28	theme	TAK1	714:717	arg1	expression					700:709	the mRNAs expression	690:709	the mRNAs expression of TAK1, p38, Osx and osteogenic markers	690:750	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	0	29	theme	pathway	60:66	arg1	research					14:21	The mechanism research	0:21	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination	0:116	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	3	30	theme	three-point	640:650	arg1	bending					652:658	three-point bending	640:658	three-point bending test	640:663	Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed.					
31776786	0	31	from	research	14:21	arg1	treatment					75:83	the treatment	71:83	the treatment of bone defects by recombination	71:116	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	4	32	theme	p38	720:722	arg1	expression					700:709	the mRNAs expression	690:709	the mRNAs expression of TAK1, p38, Osx and osteogenic markers	690:750	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	2	33	attach	released	489:496	arg2	rhBMP-2					481:487	rhBMP-2	481:487	rhBMP-2 released from the complex	481:513	METHODS Firstly, the biological activity of rhBMP-2 released from the complex was investigated.					
31776786	2	33	attach	released	489:496	arg1	complex					507:513	the complex	503:513	the complex	503:513	METHODS Firstly, the biological activity of rhBMP-2 released from the complex was investigated.					
31776786	10	34	theme	inhibitor	1411:1419	arg1	group					1421:1425	TAK1 inhibitor group	1406:1425	TAK1 inhibitor group	1406:1425	In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group.					
31776786	4	35	theme	transcription-polymerase	774:797	arg1	RT-PCR					815:820	RT-PCR	815:820	RT-PCR	815:820	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	4	35	theme	transcription-polymerase	774:797	arg1	reaction					805:812	reverse transcription-polymerase chain reaction	766:812	reverse transcription-polymerase chain reaction (RT-PCR)	766:821	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	10	36	theme	animal	1296:1301	arg1	model					1303:1307	the animal model	1292:1307	the animal model	1292:1307	In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group.					
31776786	0	37	theme	defects	93:99	arg1	treatment					75:83	the treatment	71:83	the treatment of bone defects by recombination	71:116	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	11	38	theme	mRNA	1451:1454	arg1	expression					1456:1465	the mRNA expression	1447:1465	the mRNA expression of OSX	1447:1472	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	1	39	theme	important	330:338	arg1	role					340:343	an important role	327:343	an important role	327:343	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	12	40	theme	rhBMP-2-loaded	1820:1833	arg1	composite					1861:1869	rhBMP-2-loaded hollow HA microspheres/CS composite	1820:1869	rhBMP-2-loaded hollow HA microspheres/CS composite	1820:1869	CONCLUSION The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.					
31776786	8	41	theme	microspheres/CS	1177:1191	arg1	group					1203:1207	rhBMP-2-loaded hollow HA microspheres/CS composite group	1152:1207	rhBMP-2-loaded hollow HA microspheres/CS composite group	1152:1207	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	2	42	theme	rhBMP-2	481:487	arg1	activity					469:476	the biological activity	454:476	the biological activity of rhBMP-2 released from the complex	454:513	METHODS Firstly, the biological activity of rhBMP-2 released from the complex was investigated.					
31776786	11	43	theme	inhibitor	1683:1691	arg1	group					1693:1697	p38 inhibitor group	1679:1697	p38 inhibitor group	1679:1697	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	0	44	theme	TAK1	45:48	arg1	pathway					60:66	non-Smad dependent TAK1 signaling pathway	26:66	non-Smad dependent TAK1 signaling pathway	26:66	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	12	45	theme	microspheres/CS	1845:1859	arg1	composite					1861:1869	rhBMP-2-loaded hollow HA microspheres/CS composite	1820:1869	rhBMP-2-loaded hollow HA microspheres/CS composite	1820:1869	CONCLUSION The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.					
31776786	12	46	theme	HA	1842:1843	arg1	composite					1861:1869	rhBMP-2-loaded hollow HA microspheres/CS composite	1820:1869	rhBMP-2-loaded hollow HA microspheres/CS composite	1820:1869	CONCLUSION The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.					
31776786	9	47	theme	three-point	1217:1227	arg1	test					1237:1240	the three-point bending test	1213:1240	the three-point bending test	1213:1240	In the three-point bending test, load values in p38 inhibitor group decreased.					
31776786	1	48	theme	non-Smad	239:246	arg1	pathway					311:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	1	48	theme	non-Smad	239:246	arg1	pathway					273:279	the non-Smad dependent TAK1 signaling pathway	235:279	the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway)	235:318	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	8	49	theme	bone	1124:1127	arg1	callus					1129:1134	bone callus	1124:1134	bone callus	1124:1134	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	11	50	theme	OSX	1614:1616	arg1	expression					1600:1609	the mRNA expression	1591:1609	the mRNA expression of OSX	1591:1616	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	1	51	theme	TAK1	258:261	arg1	pathway					311:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	1	51	theme	TAK1	258:261	arg1	pathway					273:279	the non-Smad dependent TAK1 signaling pathway	235:279	the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway)	235:318	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	9	52	theme	bending	1229:1235	arg1	test					1237:1240	the three-point bending test	1213:1240	the three-point bending test	1213:1240	In the three-point bending test, load values in p38 inhibitor group decreased.					
31776786	10	53	theme	p38	1382:1384	arg1	group					1396:1400	the p38 inhibitor group	1378:1400	the p38 inhibitor group	1378:1400	In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group.					
31776786	3	54	theme	osteoblasts	563:573	arg1	test					555:558	differentiation test	539:558	differentiation test of osteoblasts including ALP activity and calcium deposition	539:619	Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed.					
31776786	3	54	theme	osteoblasts	563:573	arg1	scoring					628:634	X-ray scoring	622:634	X-ray scoring	622:634	Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed.					
31776786	3	54	theme	osteoblasts	563:573	arg1	test					660:663	three-point bending test	640:663	three-point bending test	640:663	Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed.					
31776786	1	55	theme	BMP-2-TAK1-p38-Osx	282:299	arg1	pathway					311:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	1	55	theme	BMP-2-TAK1-p38-Osx	282:299	arg1	pathway					273:279	the non-Smad dependent TAK1 signaling pathway	235:279	the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway)	235:318	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	8	56	theme	hollow	1167:1172	arg1	microspheres/CS					1177:1191	rhBMP-2-loaded hollow HA microspheres/CS	1152:1191	rhBMP-2-loaded hollow HA microspheres/CS composite group	1152:1207	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	12	57	theme	bone	1782:1785	arg1	formation					1787:1795	bone formation	1782:1795	bone formation	1782:1795	CONCLUSION The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.					
31776786	9	58	from	values	1248:1253	arg1	group					1272:1276	p38 inhibitor group	1258:1276	p38 inhibitor group	1258:1276	In the three-point bending test, load values in p38 inhibitor group decreased.					
31776786	11	59	theme	inhibitor	1659:1667	arg1	group					1669:1673	TAK1 inhibitor group	1654:1673	TAK1 inhibitor group	1654:1673	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	1	60	theme	composite	426:434	arg1	HA					395:396	HA	395:396	HA	395:396	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	1	60	theme	composite	426:434	arg1	hydroxyapatite					379:392	hollow hydroxyapatite	372:392	hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	372:434	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	11	61	theme	composite	1568:1576	arg1	group					1578:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	4	62	theme	mRNAs	694:698	arg1	expression					700:709	the mRNAs expression	690:709	the mRNAs expression of TAK1, p38, Osx and osteogenic markers	690:750	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	7	63	theme	inhibitor	1081:1089	arg1	group					1091:1095	p38 inhibitor group	1077:1095	p38 inhibitor group	1077:1095	Serum ALP activity was significantly decreased in the TAK1 inhibitor group and p38 inhibitor group.					
31776786	11	64	theme	HA	1549:1550	arg1	group					1578:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	rhBMP-2-loaded hollow HA microspheres/CS composite group	1527:1582	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	3	65	theme	ALP	585:587	arg1	activity					589:596	ALP activity	585:596	ALP activity	585:596	Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed.					
31776786	0	66	theme	hydroxyapatite	138:151	arg1	composite					175:183	hollow hydroxyapatite microspheres/chitosan composite	131:183	hollow hydroxyapatite microspheres/chitosan composite	131:183	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	3	67	theme	X-ray	622:626	arg1	scoring					628:634	X-ray scoring	622:634	X-ray scoring	622:634	Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed.					
31776786	6	68	theme	time	962:965	arg1	period					952:957	a prolonged period	940:957	a prolonged period of time	940:965	Additionally, the complex provided a prolonged period of time compared with HA/CS scaffolds.					
31776786	7	69	theme	inhibitor	1057:1065	arg1	group					1067:1071	the TAK1 inhibitor group	1048:1071	the TAK1 inhibitor group	1048:1071	Serum ALP activity was significantly decreased in the TAK1 inhibitor group and p38 inhibitor group.					
31776786	9	70	theme	p38	1258:1260	arg1	group					1272:1276	p38 inhibitor group	1258:1276	p38 inhibitor group	1258:1276	In the three-point bending test, load values in p38 inhibitor group decreased.					
31776786	11	71	theme	OSX	1470:1472	arg1	expression					1456:1465	the mRNA expression	1447:1465	the mRNA expression of OSX	1447:1472	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	12	72	dep	CONCLUSION	1700:1709	arg1	play					1756:1759	play	1756:1759	may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite	1752:1869	CONCLUSION The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.					
31776786	3	73	theme	bending	652:658	arg1	test					660:663	three-point bending test	640:663	three-point bending test	640:663	Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed.					
31776786	12	74	theme	BMP-2-TAK1-p38-OSX	1715:1732	arg1	pathway					1744:1750	The BMP-2-TAK1-p38-OSX signaling pathway	1711:1750	The BMP-2-TAK1-p38-OSX signaling pathway	1711:1750	CONCLUSION The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.					
31776786	7	75	theme	Serum	998:1002	arg1	activity					1008:1015	Serum ALP activity	998:1015	Serum ALP activity	998:1015	Serum ALP activity was significantly decreased in the TAK1 inhibitor group and p38 inhibitor group.					
31776786	0	76	theme	bone	88:91	arg1	defects					93:99	bone defects	88:99	bone defects	88:99	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	1	77	theme	hollow	372:377	arg1	HA					395:396	HA	395:396	HA	395:396	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	1	77	theme	hollow	372:377	arg1	hydroxyapatite					379:392	hollow hydroxyapatite	372:392	hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	372:434	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	4	78	theme	reverse	766:772	arg1	RT-PCR					815:820	RT-PCR	815:820	RT-PCR	815:820	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	4	78	theme	reverse	766:772	arg1	reaction					805:812	reverse transcription-polymerase chain reaction	766:812	reverse transcription-polymerase chain reaction (RT-PCR)	766:821	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	8	79	located	observed	1140:1147	arg2	callus					1129:1134	bone callus	1124:1134	bone callus	1124:1134	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	8	79	located	observed	1140:1147	arg1	group					1203:1207	rhBMP-2-loaded hollow HA microspheres/CS composite group	1152:1207	rhBMP-2-loaded hollow HA microspheres/CS composite group	1152:1207	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	8	79	located	observed	1140:1147	arg1	radiography					1111:1121	the X-ray radiography	1101:1121	the X-ray radiography	1101:1121	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	4	80	theme	chain	799:803	arg1	RT-PCR					815:820	RT-PCR	815:820	RT-PCR	815:820	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	4	80	theme	chain	799:803	arg1	reaction					805:812	reverse transcription-polymerase chain reaction	766:812	reverse transcription-polymerase chain reaction (RT-PCR)	766:821	Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase chain reaction (RT-PCR).					
31776786	5	81	theme	RESULTS	824:830	arg1	RhBMP-2					832:838	RESULTS RhBMP-2	824:838	RESULTS RhBMP-2	824:838	RESULTS RhBMP-2 could be loaded and released from the complex in bioactive form.					
31776786	2	82	theme	biological	458:467	arg1	activity					469:476	the biological activity	454:476	the biological activity of rhBMP-2 released from the complex	454:513	METHODS Firstly, the biological activity of rhBMP-2 released from the complex was investigated.					
31776786	1	83	dep	AIMS	186:189	arg1	aimed					209:213	aimed	209:213	aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	209:434	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	10	84	theme	TAK1	1406:1409	arg1	group					1421:1425	TAK1 inhibitor group	1406:1425	TAK1 inhibitor group	1406:1425	In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group.					
31776786	0	85	theme	dependent	35:43	arg1	pathway					60:66	non-Smad dependent TAK1 signaling pathway	26:66	non-Smad dependent TAK1 signaling pathway	26:66	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	11	86	theme	MC3T3-E1	1431:1438	arg1	cells					1440:1444	MC3T3-E1 cells	1431:1444	MC3T3-E1 cells	1431:1444	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	8	87	theme	X-ray	1105:1109	arg1	radiography					1111:1121	the X-ray radiography	1101:1121	the X-ray radiography	1101:1121	In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA microspheres/CS composite group.					
31776786	11	88	theme	p38	1679:1681	arg1	group					1693:1697	p38 inhibitor group	1679:1697	p38 inhibitor group	1679:1697	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	0	89	theme	signaling	50:58	arg1	pathway					60:66	non-Smad dependent TAK1 signaling pathway	26:66	non-Smad dependent TAK1 signaling pathway	26:66	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	0	90	theme	hollow	131:136	arg1	composite					175:183	hollow hydroxyapatite microspheres/chitosan composite	131:183	hollow hydroxyapatite microspheres/chitosan composite	131:183	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	10	91	theme	BSP	1333:1335	arg1	expression					1319:1328	the mRNA expression	1310:1328	the mRNA expression of BSP on day 90	1310:1345	In the animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1 inhibitor group.					
31776786	1	92	theme	bone	348:351	arg1	repair					353:358	bone repair	348:358	bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite	348:434	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	12	93	theme	hollow	1835:1840	arg1	composite					1861:1869	rhBMP-2-loaded hollow HA microspheres/CS composite	1820:1869	rhBMP-2-loaded hollow HA microspheres/CS composite	1820:1869	CONCLUSION The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.					
31776786	3	94	theme	differentiation	539:553	arg1	test					555:558	differentiation test	539:558	differentiation test of osteoblasts including ALP activity and calcium deposition	539:619	Then, differentiation test of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed.					
31776786	1	95	theme	dependent	248:256	arg1	pathway					311:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	1	95	theme	dependent	248:256	arg1	pathway					273:279	the non-Smad dependent TAK1 signaling pathway	235:279	the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway)	235:318	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	5	96	attach	released	860:867	arg1	complex					878:884	the complex	874:884	the complex	874:884	RESULTS RhBMP-2 could be loaded and released from the complex in bioactive form.					
31776786	5	96	attach	released	860:867	arg2	RhBMP-2					832:838	RESULTS RhBMP-2	824:838	RESULTS RhBMP-2	824:838	RESULTS RhBMP-2 could be loaded and released from the complex in bioactive form.					
31776786	11	97	theme	mRNA	1595:1598	arg1	expression					1600:1609	the mRNA expression	1591:1609	the mRNA expression of OSX	1591:1616	In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly down-regulated in TAK1 inhibitor group and p38 inhibitor group.					
31776786	0	98	theme	mechanism	4:12	arg1	research					14:21	The mechanism research	0:21	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination	0:116	The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2-loaded hollow hydroxyapatite microspheres/chitosan composite.					
31776786	1	99	theme	signaling	263:271	arg1	pathway					311:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	BMP-2-TAK1-p38-Osx signaling pathway	282:317	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31776786	1	99	theme	signaling	263:271	arg1	pathway					273:279	the non-Smad dependent TAK1 signaling pathway	235:279	the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway)	235:318	AIMS The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/chitosan (CS) composite.					
31546410	6	0	theme	Thermo-Gravimetric	1293:1310	arg1	TGA					1322:1324	TGA	1322:1324	TGA	1322:1324	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	0	theme	Thermo-Gravimetric	1293:1310	arg1	Analysis					1312:1319	Thermo-Gravimetric Analysis	1293:1319	Thermo-Gravimetric Analysis (TGA)	1293:1325	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	0	1	theme	oxazolidin-2-ones	199:215	arg1	synthesis					171:179	regio- and stereoselective synthesis	144:179	regio- and stereoselective synthesis of functionalized oxazolidin-2-ones	144:215	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	2	2	with	compounds	542:550	arg1	activity					568:575	biological activity	557:575	biological activity	557:575	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	6	3	theme	Electron	1365:1372	arg1	TEM					1386:1388	TEM	1386:1388	TEM	1386:1388	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	3	theme	Electron	1365:1372	arg1	Microscopy					1374:1383	Transmission Electron Microscopy	1352:1383	Transmission Electron Microscopy (TEM)	1352:1389	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	4	4	theme	other	966:970	arg1	catalysts					986:994	other heterogeneous catalysts	966:994	other heterogeneous catalysts	966:994	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	2	5	theme	biological	557:566	arg1	activity					568:575	biological activity	557:575	biological activity	557:575	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	0	6	theme	functionalized	184:197	arg1	oxazolidin-2-ones					199:215	functionalized oxazolidin-2-ones	184:215	functionalized oxazolidin-2-ones	184:215	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	1	7	theme	MnFe2O4	309:315	arg1	acid					303:306	MnFe2O4/chitosan/phosphotungstic acid	270:306	MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA)	270:323	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	1	7	theme	MnFe2O4	309:315	arg1	PTA					320:322	MnFe2O4@CS@PTA	309:322	MnFe2O4@CS@PTA	309:322	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	5	8	theme	MnFe2O4	1022:1028	arg1	substance					1060:1068	a hopeful catalytic substance	1040:1068	a hopeful catalytic substance for mass fine chemicals production	1040:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	8	theme	MnFe2O4	1022:1028	arg1	PTA					1033:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	9	theme	catalytic	1050:1058	arg1	substance					1060:1068	a hopeful catalytic substance	1040:1068	a hopeful catalytic substance for mass fine chemicals production	1040:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	9	theme	catalytic	1050:1058	arg1	PTA					1033:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	10	theme	mass	1074:1077	arg1	chemicals					1084:1092	mass fine chemicals	1074:1092	mass fine chemicals production	1074:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	11	theme	chemicals	1084:1092	arg1	production					1094:1103	mass fine chemicals production	1074:1103	mass fine chemicals production	1074:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	12	theme	@	1032:1032	arg1	substance					1060:1068	a hopeful catalytic substance	1040:1068	a hopeful catalytic substance for mass fine chemicals production	1040:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	12	theme	@	1032:1032	arg1	PTA					1033:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	4	13	from	stability	861:869	arg1	solvent					819:825	protic solvent	812:825	protic solvent	812:825	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	1	14	theme	@	316:316	arg1	acid					303:306	MnFe2O4/chitosan/phosphotungstic acid	270:306	MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA)	270:323	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	1	14	theme	@	316:316	arg1	PTA					320:322	MnFe2O4@CS@PTA	309:322	MnFe2O4@CS@PTA	309:322	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	0	15	theme	novel	94:98	arg1	nanocatalyst					127:138	a novel and highly effective green nanocatalyst	92:138	a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones	92:215	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	4	16	theme	useful	944:949	arg1	alternative					951:961	a useful alternative	942:961	a useful alternative to other heterogeneous catalysts	942:994	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	4	17	from	preparation	840:850	arg1	solvent					819:825	protic solvent	812:825	protic solvent	812:825	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	4	18	theme	easy	835:838	arg1	preparation					840:850	the easy preparation	831:850	the easy preparation	831:850	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	1	19	theme	CS	317:318	arg1	acid					303:306	MnFe2O4/chitosan/phosphotungstic acid	270:306	MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA)	270:323	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	1	19	theme	CS	317:318	arg1	PTA					320:322	MnFe2O4@CS@PTA	309:322	MnFe2O4@CS@PTA	309:322	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	0	20	theme	effective	111:119	arg1	nanocatalyst					127:138	a novel and highly effective green nanocatalyst	92:138	a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones	92:215	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	4	21	theme	good	912:915	arg1	system					931:936	a good heterogeneous system	910:936	a good heterogeneous system	910:936	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	4	22	theme	thermal	853:859	arg1	stability					861:869	thermal stability	853:869	thermal stability	853:869	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	6	23	theme	nanocomposite	1143:1155	arg1	structure					1110:1118	The structure	1106:1118	The structure	1106:1118	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	23	theme	nanocomposite	1143:1155	arg1	composition					1124:1134	composition	1124:1134	composition	1124:1134	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	24	theme	Vibrating	1392:1400	arg1	VSM					1423:1425	VSM	1423:1425	VSM	1423:1425	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	24	theme	Vibrating	1392:1400	arg1	Magnetometer					1409:1420	Vibrating Sample Magnetometer	1392:1420	Vibrating Sample Magnetometer (VSM)	1392:1426	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	0	25	theme	magnetic	8:15	arg1	nanoparticles					17:29	MnFe2O4 magnetic nanoparticles	0:29	MnFe2O4 magnetic nanoparticles	0:29	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	5	26	theme	synthesized	1010:1020	arg1	substance					1060:1068	a hopeful catalytic substance	1040:1068	a hopeful catalytic substance for mass fine chemicals production	1040:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	26	theme	synthesized	1010:1020	arg1	PTA					1033:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	1	27	theme	@	319:319	arg1	acid					303:306	MnFe2O4/chitosan/phosphotungstic acid	270:306	MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA)	270:323	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	1	27	theme	@	319:319	arg1	PTA					320:322	MnFe2O4@CS@PTA	309:322	MnFe2O4@CS@PTA	309:322	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	3	28	theme	reaction	745:752	arg1	time					754:757	short reaction time	739:757	short reaction time	739:757	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	4	29	theme	protic	812:817	arg1	solvent					819:825	protic solvent	812:825	protic solvent	812:825	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	6	30	theme	Electron	1263:1270	arg1	Microscopy					1272:1281	Field Emission Scanning Electron Microscopy	1239:1281	Field Emission Scanning Electron Microscopy (FE-SEM)	1239:1290	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	30	theme	Electron	1263:1270	arg1	FE-SEM					1284:1289	FE-SEM	1284:1289	FE-SEM	1284:1289	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	2	31	with	α-epoxyketones	601:614	arg1	thiourea					630:637	thiourea	630:637	thiourea	630:637	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	2	31	with	α-epoxyketones	601:614	arg1	urea					621:624	urea	621:624	urea	621:624	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	5	32	theme	@	1029:1029	arg1	substance					1060:1068	a hopeful catalytic substance	1040:1068	a hopeful catalytic substance for mass fine chemicals production	1040:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	32	theme	@	1029:1029	arg1	PTA					1033:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	33	theme	CS	1030:1031	arg1	substance					1060:1068	a hopeful catalytic substance	1040:1068	a hopeful catalytic substance for mass fine chemicals production	1040:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	33	theme	CS	1030:1031	arg1	PTA					1033:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	2	34	theme	versatile	481:489	arg1	synthons					498:505	versatile chiral synthons	481:505	versatile chiral synthons	481:505	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	2	35	theme	chiral	491:496	arg1	synthons					498:505	versatile chiral synthons	481:505	versatile chiral synthons	481:505	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	3	36	contain	has	659:661	arg2	yields					700:705	excellent yields	690:705	excellent yields	690:705	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	3	36	contain	has	659:661	arg2	time					754:757	short reaction time	739:757	short reaction time	739:757	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	3	36	contain	has	659:661	arg1	procedure					649:657	This new procedure	640:657	This new procedure	640:657	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	3	36	contain	has	659:661	arg2	conditions					723:732	green reaction conditions	708:732	green reaction conditions	708:732	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	3	36	contain	has	659:661	arg2	advantages					671:680	notable advantages	663:680	notable advantages such as excellent yields, green reaction conditions, and short reaction time	663:757	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	0	37	theme	green	121:125	arg1	nanocatalyst					127:138	a novel and highly effective green nanocatalyst	92:138	a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones	92:215	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	6	38	theme	Emission	1245:1252	arg1	Microscopy					1272:1281	Field Emission Scanning Electron Microscopy	1239:1281	Field Emission Scanning Electron Microscopy (FE-SEM)	1239:1290	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	38	theme	Emission	1245:1252	arg1	FE-SEM					1284:1289	FE-SEM	1284:1289	FE-SEM	1284:1289	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	3	39	theme	new	645:647	arg1	procedure					649:657	This new procedure	640:657	This new procedure	640:657	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	6	40	theme	different	1174:1182	arg1	methods					1184:1190	different methods	1174:1190	different methods	1174:1190	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	0	41	theme	regio-	144:149	arg1	synthesis					171:179	regio- and stereoselective synthesis	144:179	regio- and stereoselective synthesis of functionalized oxazolidin-2-ones	144:215	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	4	42	theme	catalyst	893:900	arg1	separation					875:884	separation	875:884	separation	875:884	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	4	42	theme	catalyst	893:900	arg1	preparation					840:850	the easy preparation	831:850	the easy preparation	831:850	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	4	42	theme	catalyst	893:900	arg1	stability					861:869	thermal stability	853:869	thermal stability	853:869	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	4	42	theme	catalyst	893:900	arg1	performance					797:807	the excellent catalytic performance	773:807	the excellent catalytic performance in protic solvent	773:825	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	1	43	theme	recoverable	239:249	arg1	nanocatalyst					256:267	A novel magnetically recoverable acid nanocatalyst	218:267	A novel magnetically recoverable acid nanocatalyst	218:267	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	1	43	theme	recoverable	239:249	arg1	acid					303:306	MnFe2O4/chitosan/phosphotungstic acid	270:306	MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA)	270:323	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	3	44	theme	reaction	714:721	arg1	conditions					723:732	green reaction conditions	708:732	green reaction conditions	708:732	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	5	45	theme	fine	1079:1082	arg1	chemicals					1084:1092	mass fine chemicals	1074:1092	mass fine chemicals production	1074:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	4	46	from	separation	875:884	arg1	solvent					819:825	protic solvent	812:825	protic solvent	812:825	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	2	47	theme	functionalized	445:458	arg1	oxazolidin-2-ones					460:476	the functionalized oxazolidin-2-ones	441:476	the functionalized oxazolidin-2-ones as versatile chiral synthons	441:505	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	0	48	theme	stereoselective	155:169	arg1	synthesis					171:179	regio- and stereoselective synthesis	144:179	regio- and stereoselective synthesis of functionalized oxazolidin-2-ones	144:215	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	3	49	theme	notable	663:669	arg1	yields					700:705	excellent yields	690:705	excellent yields	690:705	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	3	49	theme	notable	663:669	arg1	time					754:757	short reaction time	739:757	short reaction time	739:757	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	3	49	theme	notable	663:669	arg1	advantages					671:680	notable advantages	663:680	notable advantages such as excellent yields, green reaction conditions, and short reaction time	663:757	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	3	49	theme	notable	663:669	arg1	conditions					723:732	green reaction conditions	708:732	green reaction conditions	708:732	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	4	50	theme	heterogeneous	917:929	arg1	system					931:936	a good heterogeneous system	910:936	a good heterogeneous system	910:936	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	4	51	theme	catalytic	787:795	arg1	performance					797:807	the excellent catalytic performance	773:807	the excellent catalytic performance in protic solvent	773:825	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	0	52	theme	polymeric	54:62	arg1	chitosan					45:52	chitosan polymeric	45:62	chitosan polymeric	45:62	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	6	53	theme	Field	1239:1243	arg1	Microscopy					1272:1281	Field Emission Scanning Electron Microscopy	1239:1281	Field Emission Scanning Electron Microscopy (FE-SEM)	1239:1290	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	53	theme	Field	1239:1243	arg1	FE-SEM					1284:1289	FE-SEM	1284:1289	FE-SEM	1284:1289	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	54	theme	Transmission	1352:1363	arg1	TEM					1386:1388	TEM	1386:1388	TEM	1386:1388	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	54	theme	Transmission	1352:1363	arg1	Microscopy					1374:1383	Transmission Electron Microscopy	1352:1383	Transmission Electron Microscopy (TEM)	1352:1389	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	4	55	theme	excellent	777:785	arg1	performance					797:807	the excellent catalytic performance	773:807	the excellent catalytic performance in protic solvent	773:825	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	5	56	theme	hopeful	1042:1048	arg1	substance					1060:1068	a hopeful catalytic substance	1040:1068	a hopeful catalytic substance for mass fine chemicals production	1040:1103	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	5	56	theme	hopeful	1042:1048	arg1	PTA					1033:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	the synthesized MnFe2O4@CS@PTA	1006:1035	Perhaps, the synthesized MnFe2O4@CS@PTA is a hopeful catalytic substance for mass fine chemicals production.					
31546410	2	57	theme	synthesized	360:370	arg1	nanoparticle					372:383	The synthesized nanoparticle	356:383	The synthesized nanoparticle	356:383	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	2	57	theme	synthesized	360:370	arg1	nanocatalyst					417:428	the heterogenous nanocatalyst	400:428	the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea	400:637	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	4	58	from	performance	797:807	arg1	solvent					819:825	protic solvent	812:825	protic solvent	812:825	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	4	59	theme	heterogeneous	972:984	arg1	catalysts					986:994	other heterogeneous catalysts	966:994	other heterogeneous catalysts	966:994	In addition, the excellent catalytic performance in protic solvent and the easy preparation, thermal stability and separation of the catalyst make it a good heterogeneous system and a useful alternative to other heterogeneous catalysts.					
31546410	3	60	theme	short	739:743	arg1	time					754:757	short reaction time	739:757	short reaction time	739:757	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	3	61	theme	green	708:712	arg1	conditions					723:732	green reaction conditions	708:732	green reaction conditions	708:732	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	6	62	theme	Sample	1402:1407	arg1	VSM					1423:1425	VSM	1423:1425	VSM	1423:1425	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	62	theme	Sample	1402:1407	arg1	Magnetometer					1409:1420	Vibrating Sample Magnetometer	1392:1420	Vibrating Sample Magnetometer (VSM)	1392:1426	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	1	63	theme	acid	251:254	arg1	nanocatalyst					256:267	A novel magnetically recoverable acid nanocatalyst	218:267	A novel magnetically recoverable acid nanocatalyst	218:267	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	1	63	theme	acid	251:254	arg1	acid					303:306	MnFe2O4/chitosan/phosphotungstic acid	270:306	MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA)	270:323	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	3	64	theme	excellent	690:698	arg1	yields					700:705	excellent yields	690:705	excellent yields	690:705	This new procedure has notable advantages such as excellent yields, green reaction conditions, and short reaction time.					
31546410	1	65	theme	novel	220:224	arg1	nanocatalyst					256:267	A novel magnetically recoverable acid nanocatalyst	218:267	A novel magnetically recoverable acid nanocatalyst	218:267	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	1	65	theme	novel	220:224	arg1	acid					303:306	MnFe2O4/chitosan/phosphotungstic acid	270:306	MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA)	270:323	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	2	66	theme	heterogenous	404:415	arg1	nanoparticle					372:383	The synthesized nanoparticle	356:383	The synthesized nanoparticle	356:383	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	2	66	theme	heterogenous	404:415	arg1	nanocatalyst					417:428	the heterogenous nanocatalyst	400:428	the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea	400:637	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
31546410	1	67	theme	MnFe2O4/chitosan/phosphotungstic	270:301	arg1	nanocatalyst					256:267	A novel magnetically recoverable acid nanocatalyst	218:267	A novel magnetically recoverable acid nanocatalyst	218:267	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	1	67	theme	MnFe2O4/chitosan/phosphotungstic	270:301	arg1	acid					303:306	MnFe2O4/chitosan/phosphotungstic acid	270:306	MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA)	270:323	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	1	67	theme	MnFe2O4/chitosan/phosphotungstic	270:301	arg1	PTA					320:322	MnFe2O4@CS@PTA	309:322	MnFe2O4@CS@PTA	309:322	A novel magnetically recoverable acid nanocatalyst, MnFe2O4/chitosan/phosphotungstic acid (MnFe2O4@CS@PTA), was successfully synthesized.					
31546410	0	68	theme	phosphotungstic	68:82	arg1	acid					84:87	phosphotungstic acid	68:87	phosphotungstic acid	68:87	MnFe2O4 magnetic nanoparticles modified with chitosan polymeric and phosphotungstic acid as a novel and highly effective green nanocatalyst for regio- and stereoselective synthesis of functionalized oxazolidin-2-ones.					
31546410	6	69	theme	Infrared	1208:1215	arg1	XRD					1347:1349	XRD	1347:1349	XRD	1347:1349	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	69	theme	Infrared	1208:1215	arg1	FT-IR					1231:1235	FT-IR	1231:1235	FT-IR	1231:1235	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	69	theme	Infrared	1208:1215	arg1	Spectroscopy					1217:1228	Infrared Spectroscopy	1208:1228	Infrared Spectroscopy (FT-IR)	1208:1236	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	70	theme	Scanning	1254:1261	arg1	Microscopy					1272:1281	Field Emission Scanning Electron Microscopy	1239:1281	Field Emission Scanning Electron Microscopy (FE-SEM)	1239:1290	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	70	theme	Scanning	1254:1261	arg1	FE-SEM					1284:1289	FE-SEM	1284:1289	FE-SEM	1284:1289	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	6	71	theme	X-ray	1328:1332	arg1	Diffraction					1334:1344	X-ray Diffraction	1328:1344	X-ray Diffraction	1328:1344	The structure and composition of the nanocomposite was performed by different methods and analyzed by Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM), Thermo-Gravimetric Analysis (TGA), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM), Vibrating Sample Magnetometer (VSM) and Brunauere Emmette Teller (BET).					
31546410	2	72	theme	α-epoxyketones	601:614	arg1	reaction					589:596	the reaction	585:596	the reaction of α-epoxyketones with urea and thiourea	585:637	The synthesized nanoparticle was studied as the heterogenous nanocatalyst to prepare the functionalized oxazolidin-2-ones as versatile chiral synthons in asymmetrically synthesizing the compounds with biological activity through the reaction of α-epoxyketones with urea and thiourea.					
30948108	0	0	theme	coated	78:83	arg1	alloy					95:99	a chitosan-bioactive glass coated magnesium alloy	51:99	a chitosan-bioactive glass coated magnesium alloy	51:99	Influence of proteins on the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy.					
30948108	5	1	from	proteins	949:956	arg1	solution					961:968	solution	961:968	solution	961:968	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	0	2	theme	glass	72:76	arg1	alloy					95:99	a chitosan-bioactive glass coated magnesium alloy	51:99	a chitosan-bioactive glass coated magnesium alloy	51:99	Influence of proteins on the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy.					
30948108	3	3	theme	immersion	602:610	arg1	7d					596:597	7d	596:597	7d	596:597	Mass-loss was measured to determine the corrosion rate after 3d and 7d of immersion.					
30948108	3	3	theme	immersion	602:610	arg1	3d					589:590	3d	589:590	3d	589:590	Mass-loss was measured to determine the corrosion rate after 3d and 7d of immersion.					
30948108	5	4	theme	serum	829:833	arg1	addition					835:842	serum addition	829:842	serum addition	829:842	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	6	5	from	immersion	1129:1137	arg1	presence					1158:1165	presence	1158:1165	presence	1158:1165	These results are discussed in correlation to observed changes in surface chemistry taking place upon immersion in the absence and presence of proteins.					
30948108	6	5	from	immersion	1129:1137	arg1	absence					1146:1152	absence	1146:1152	absence	1146:1152	These results are discussed in correlation to observed changes in surface chemistry taking place upon immersion in the absence and presence of proteins.					
30948108	0	6	theme	alloy	95:99	arg1	behavior					39:46	the corrosion behavior	25:46	the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy	25:99	Influence of proteins on the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy.					
30948108	0	7	from	Influence	0:8	arg1	behavior					39:46	the corrosion behavior	25:46	the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy	25:99	Influence of proteins on the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy.					
30948108	1	8	theme	chitosan-bioactive	368:385	arg1	glass					387:391	chitosan-bioactive glass	368:391	chitosan-bioactive glass	368:391	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	0	9	theme	magnesium	85:93	arg1	alloy					95:99	a chitosan-bioactive glass coated magnesium alloy	51:99	a chitosan-bioactive glass coated magnesium alloy	51:99	Influence of proteins on the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy.					
30948108	2	10	theme	degradation	507:517	arg1	process					519:525	the degradation process	503:525	the degradation process	503:525	The immersion tests were conducted with and without addition of serum, to study the influence of proteins on the degradation process.					
30948108	4	11	theme	surface	668:674	arg1	morphology					676:685	their surface morphology	662:685	their surface morphology	662:685	The samples were analyzed by SEM with respect to their surface morphology and the chemical composition was screened by high-resolution XPS.					
30948108	5	12	theme	addition	835:842	arg1	influence					816:824	a significant, time-dependent influence	786:824	a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied	786:893	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	5	13	from	influence	816:824	arg1	behavior					861:868	the corrosion behavior	847:868	the corrosion behavior of the materials studied	847:893	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	5	14	theme	significant	788:798	arg1	influence					816:824	a significant, time-dependent influence	786:824	a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied	786:893	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	1	15	theme	WE43	159:162	arg1	Mg					164:165	Mg	164:165	Mg	164:165	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	6	16	theme	proteins	1170:1177	arg1	presence					1158:1165	presence	1158:1165	presence	1158:1165	These results are discussed in correlation to observed changes in surface chemistry taking place upon immersion in the absence and presence of proteins.					
30948108	6	16	theme	proteins	1170:1177	arg1	absence					1146:1152	absence	1146:1152	absence	1146:1152	These results are discussed in correlation to observed changes in surface chemistry taking place upon immersion in the absence and presence of proteins.					
30948108	1	17	theme	Mg	164:165	arg1	alloy					167:171	a WE43 Mg alloy	157:171	a WE43 Mg alloy	157:171	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	6	18	theme	observed	1073:1080	arg1	changes					1082:1088	observed changes	1073:1088	observed changes in surface chemistry taking place upon immersion in the absence and presence of proteins	1073:1177	These results are discussed in correlation to observed changes in surface chemistry taking place upon immersion in the absence and presence of proteins.					
30948108	1	19	theme	alloy	167:171	arg1	behavior					145:152	the degradation behavior	129:152	the degradation behavior of a WE43 Mg alloy	129:171	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	5	20	dep	significant	788:798	arg1	time-dependent					801:814	time-dependent	801:814	time-dependent	801:814	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	1	21	theme	immersion	180:188	arg1	tests					190:194	immersion tests	180:194	immersion tests	180:194	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	0	22	theme	proteins	13:20	arg1	Influence					0:8	Influence	0:8	Influence of proteins on the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy.	0:100	Influence of proteins on the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy.					
30948108	1	23	theme	surface	309:315	arg1	pre-treatment					317:329	surface pre-treatment	309:329	surface pre-treatment	309:329	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	5	24	theme	materials	877:885	arg1	behavior					861:868	the corrosion behavior	847:868	the corrosion behavior of the materials studied	847:893	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	0	25	theme	corrosion	29:37	arg1	behavior					39:46	the corrosion behavior	25:46	the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy	25:99	Influence of proteins on the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy.					
30948108	6	26	from	changes	1082:1088	arg1	chemistry					1101:1109	surface chemistry	1093:1109	surface chemistry taking place upon immersion in the absence and presence of proteins	1093:1177	These results are discussed in correlation to observed changes in surface chemistry taking place upon immersion in the absence and presence of proteins.					
30948108	4	27	theme	high-resolution	732:746	arg1	XPS					748:750	high-resolution XPS	732:750	high-resolution XPS	732:750	The samples were analyzed by SEM with respect to their surface morphology and the chemical composition was screened by high-resolution XPS.					
30948108	5	28	theme	sample	936:941	arg1	type					943:946	the sample type	932:946	the sample type	932:946	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	2	29	theme	immersion	398:406	arg1	tests					408:412	The immersion tests	394:412	The immersion tests	394:412	The immersion tests were conducted with and without addition of serum, to study the influence of proteins on the degradation process.					
30948108	3	30	theme	corrosion	568:576	arg1	rate					578:581	the corrosion rate	564:581	the corrosion rate	564:581	Mass-loss was measured to determine the corrosion rate after 3d and 7d of immersion.					
30948108	1	31	theme	current	106:112	arg1	study					114:118	The current study	102:118	The current study	102:118	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	6	32	theme	surface	1093:1099	arg1	chemistry					1101:1109	surface chemistry	1093:1109	surface chemistry taking place upon immersion in the absence and presence of proteins	1093:1177	These results are discussed in correlation to observed changes in surface chemistry taking place upon immersion in the absence and presence of proteins.					
30948108	6	33	dep	absence	1146:1152	arg1	the					1142:1144	the	1142:1144	the	1142:1144	These results are discussed in correlation to observed changes in surface chemistry taking place upon immersion in the absence and presence of proteins.					
30948108	1	34	with	samples	296:302	arg1	pre-treatment					317:329	surface pre-treatment	309:329	surface pre-treatment	309:329	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	1	35	theme	bare	262:265	arg1	surface					273:279	a bare alloy surface	260:279	a bare alloy surface	260:279	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	2	36	theme	proteins	491:498	arg1	influence					478:486	the influence	474:486	the influence of proteins on the degradation process	474:525	The immersion tests were conducted with and without addition of serum, to study the influence of proteins on the degradation process.					
30948108	5	37	dep	demonstrate	765:775	arg1	only					781:784	only	781:784	only	781:784	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	4	38	theme	chemical	695:702	arg1	composition					704:714	the chemical composition	691:714	the chemical composition	691:714	The samples were analyzed by SEM with respect to their surface morphology and the chemical composition was screened by high-resolution XPS.					
30948108	5	39	theme	corrosion	851:859	arg1	behavior					861:868	the corrosion behavior	847:868	the corrosion behavior of the materials studied	847:893	The results demonstrate not only a significant, time-dependent influence of serum addition on the corrosion behavior of the materials studied, but noteworthy is that depending on the sample type, proteins in solution were observed to either accelerate or inhibit corrosion.					
30948108	1	40	theme	Modified	210:217	arg1	DMEM					235:238	DMEM	235:238	DMEM	235:238	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	1	40	theme	Modified	210:217	arg1	Medium					227:232	Dulbecco's Modified Eagle's Medium	199:232	Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d	199:253	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	0	41	theme	chitosan-bioactive	53:70	arg1	alloy					95:99	a chitosan-bioactive glass coated magnesium alloy	51:99	a chitosan-bioactive glass coated magnesium alloy	51:99	Influence of proteins on the corrosion behavior of a chitosan-bioactive glass coated magnesium alloy.					
30948108	1	42	theme	degradation	133:143	arg1	behavior					145:152	the degradation behavior	129:152	the degradation behavior of a WE43 Mg alloy	129:171	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	2	43	from	influence	478:486	arg1	process					519:525	the degradation process	503:525	the degradation process	503:525	The immersion tests were conducted with and without addition of serum, to study the influence of proteins on the degradation process.					
30948108	1	44	theme	alloy	267:271	arg1	surface					273:279	a bare alloy surface	260:279	a bare alloy surface	260:279	The current study explored the degradation behavior of a WE43 Mg alloy during immersion tests in Dulbecco's Modified Eagle's Medium (DMEM) for 3d and 7d, for a bare alloy surface as well as for samples with surface pre-treatment, and finally for samples coated with chitosan-bioactive glass.					
30948108	2	45	theme	serum	458:462	arg1	addition					446:453	addition	446:453	addition of serum	446:462	The immersion tests were conducted with and without addition of serum, to study the influence of proteins on the degradation process.					
30556278	3	0	theme	agar	396:399	arg1	microspheres					401:412	The agar microspheres	392:412	The agar microspheres grafted with copolymers	392:436	The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy.					
30556278	4	1	theme	significant	696:706	arg1	effects					708:714	significant effects	696:714	significant effects on the hydrophobicity and adsorption capacity of agar microspheres	696:781	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	6	2	theme	all-aqueous	1082:1092	arg1	bioseparations					1094:1107	all-aqueous bioseparations	1082:1107	all-aqueous bioseparations	1082:1107	Thus, we suggest thermoresponsive agar microspheres as an alternative separation media for all-aqueous bioseparations.					
30556278	5	3	theme	proteins	872:879	arg1	separation					858:867	separation	858:867	separation of proteins	858:879	The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition.					
30556278	6	4	theme	separation	1061:1070	arg1	media					1072:1076	separation media	1061:1076	an alternative separation media for all-aqueous bioseparations	1046:1107	Thus, we suggest thermoresponsive agar microspheres as an alternative separation media for all-aqueous bioseparations.					
30556278	3	5	theme	elemental	484:492	arg1	analysis					494:501	elemental analysis	484:501	elemental analysis	484:501	The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy.					
30556278	2	6	theme	transfer	359:366	arg1	polymerization					376:389	atom transfer radical polymerization	354:389	atom transfer radical polymerization	354:389	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	4	7	from	effects	708:714	arg1	hydrophobicity					723:736	hydrophobicity	723:736	hydrophobicity	723:736	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	4	7	from	effects	708:714	arg1	capacity					753:760	adsorption capacity	742:760	adsorption capacity	742:760	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	2	8	theme	atom	354:357	arg1	polymerization					376:389	atom transfer radical polymerization	354:389	atom transfer radical polymerization	354:389	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	4	9	theme	different	786:794	arg1	temperatures					796:807	different temperatures	786:807	different temperatures	786:807	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	5	10	from	change	955:960	arg1	composition					978:988	mobile phase composition	965:988	mobile phase composition	965:988	The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition.					
30556278	1	11	theme	Agar	100:103	arg1	microspheres					105:116	Agar microspheres	100:116	Agar microspheres	100:116	Agar microspheres were prepared by water-oil emulsification and cross-linked under alkaline condition.					
30556278	3	12	theme	scanning	545:552	arg1	microscopy					563:572	scanning electron microscopy	545:572	scanning electron microscopy	545:572	The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy.					
30556278	0	13	theme	hydrophobic	17:27	arg1	copolymer					29:37	Thermoresponsive hydrophobic copolymer	0:37	Thermoresponsive hydrophobic copolymer	0:37	Thermoresponsive hydrophobic copolymer grafted on agar microspheres for all-aqueous bioseparations.					
30556278	3	14	dep	Fourier	504:510	arg1	transform					512:520	transform	512:520	transform infrared spectroscopy	512:542	The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy.					
30556278	5	15	dep	binding	892:898	arg1	behavior					912:919	behavior	912:919	behavior	912:919	The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition.					
30556278	4	16	theme	microspheres	770:781	arg1	effects					708:714	significant effects	696:714	significant effects on the hydrophobicity and adsorption capacity of agar microspheres	696:781	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	0	17	theme	Thermoresponsive	0:15	arg1	copolymer					29:37	Thermoresponsive hydrophobic copolymer	0:37	Thermoresponsive hydrophobic copolymer	0:37	Thermoresponsive hydrophobic copolymer grafted on agar microspheres for all-aqueous bioseparations.					
30556278	4	18	theme	grafting	668:675	arg1	polymer					684:690	the grafting linear polymer	664:690	the grafting linear polymer	664:690	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	3	19	dep	transform	512:520	arg1	infrared					522:529	infrared	522:529	transform infrared spectroscopy	512:542	The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy.					
30556278	6	20	theme	thermoresponsive	1008:1023	arg1	microspheres					1030:1041	thermoresponsive agar microspheres	1008:1041	thermoresponsive agar microspheres	1008:1041	Thus, we suggest thermoresponsive agar microspheres as an alternative separation media for all-aqueous bioseparations.					
30556278	6	20	theme	thermoresponsive	1008:1023	arg1	alternative					1049:1059	an alternative separation media	1046:1076	an alternative separation media for all-aqueous bioseparations	1046:1107	Thus, we suggest thermoresponsive agar microspheres as an alternative separation media for all-aqueous bioseparations.					
30556278	3	21	theme	light	460:464	arg1	microphotography					466:481	light microphotography	460:481	light microphotography	460:481	The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy.					
30556278	5	22	theme	phase	972:976	arg1	composition					978:988	mobile phase composition	965:988	mobile phase composition	965:988	The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition.					
30556278	4	23	theme	polymer	684:690	arg1	ratio					655:659	hydrophobic monomer ratio	635:659	hydrophobic monomer ratio	635:659	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	4	23	theme	polymer	684:690	arg1	lengths					623:629	chain lengths	617:629	chain lengths	617:629	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	6	24	theme	agar	1025:1028	arg1	microspheres					1030:1041	thermoresponsive agar microspheres	1008:1041	thermoresponsive agar microspheres	1008:1041	Thus, we suggest thermoresponsive agar microspheres as an alternative separation media for all-aqueous bioseparations.					
30556278	6	24	theme	agar	1025:1028	arg1	alternative					1049:1059	an alternative separation media	1046:1076	an alternative separation media for all-aqueous bioseparations	1046:1107	Thus, we suggest thermoresponsive agar microspheres as an alternative separation media for all-aqueous bioseparations.					
30556278	2	25	theme	radical	368:374	arg1	polymerization					376:389	atom transfer radical polymerization	354:389	atom transfer radical polymerization	354:389	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	0	26	theme	agar	50:53	arg1	microspheres					55:66	agar microspheres	50:66	agar microspheres for all-aqueous bioseparations	50:97	Thermoresponsive hydrophobic copolymer grafted on agar microspheres for all-aqueous bioseparations.					
30556278	4	27	theme	linear	677:682	arg1	polymer					684:690	the grafting linear polymer	664:690	the grafting linear polymer	664:690	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	2	28	theme	hydrophobic	224:234	arg1	poly					247:250	poly	247:250	poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide)	247:310	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	2	28	theme	hydrophobic	224:234	arg1	copolymer					236:244	The thermoresponsive hydrophobic copolymer	203:244	The thermoresponsive hydrophobic copolymer	203:244	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	3	29	theme	electron	554:561	arg1	microscopy					563:572	scanning electron microscopy	545:572	scanning electron microscopy	545:572	The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy.					
30556278	4	30	theme	monomer	647:653	arg1	ratio					655:659	hydrophobic monomer ratio	635:659	hydrophobic monomer ratio	635:659	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	2	31	theme	thermoresponsive	207:222	arg1	poly					247:250	poly	247:250	poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide)	247:310	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	2	31	theme	thermoresponsive	207:222	arg1	copolymer					236:244	The thermoresponsive hydrophobic copolymer	203:244	The thermoresponsive hydrophobic copolymer	203:244	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	5	32	theme	mobile	965:970	arg1	composition					978:988	mobile phase composition	965:988	mobile phase composition	965:988	The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition.					
30556278	4	33	theme	agar	765:768	arg1	microspheres					770:781	agar microspheres	765:781	agar microspheres	765:781	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	5	34	theme	temperatures	934:945	arg1	release					904:910	release	904:910	release	904:910	The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition.					
30556278	5	34	theme	temperatures	934:945	arg1	binding					892:898	binding	892:898	binding	892:898	The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition.					
30556278	4	35	theme	hydrophobic	635:645	arg1	ratio					655:659	hydrophobic monomer ratio	635:659	hydrophobic monomer ratio	635:659	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	4	36	contain	had	692:694	arg1	ratio					655:659	hydrophobic monomer ratio	635:659	hydrophobic monomer ratio	635:659	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	4	36	contain	had	692:694	arg1	lengths					623:629	chain lengths	617:629	chain lengths	617:629	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	4	36	contain	had	692:694	arg2	effects					708:714	significant effects	696:714	significant effects on the hydrophobicity and adsorption capacity of agar microspheres	696:781	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	1	37	theme	water-oil	135:143	arg1	emulsification					145:158	water-oil emulsification	135:158	water-oil emulsification	135:158	Agar microspheres were prepared by water-oil emulsification and cross-linked under alkaline condition.					
30556278	5	38	used	used	849:852	arg2	microspheres					831:842	The thermoresponsive microspheres	810:842	The thermoresponsive microspheres	810:842	The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition.					
30556278	5	39	theme	thermoresponsive	814:829	arg1	microspheres					831:842	The thermoresponsive microspheres	810:842	The thermoresponsive microspheres	810:842	The thermoresponsive microspheres were used for separation of proteins and showed binding and release behavior by change of temperatures without change in mobile phase composition.					
30556278	2	40	theme	N-isopropylacrylamide-co-lauryl	252:282	arg1	poly					247:250	poly	247:250	poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide)	247:310	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	2	40	theme	N-isopropylacrylamide-co-lauryl	252:282	arg1	methacrylate-co-acrylamide					284:309	N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide	252:309	N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide	252:309	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	6	41	dep	alternative	1049:1059	arg1	media					1072:1076	separation media	1061:1076	an alternative separation media for all-aqueous bioseparations	1046:1107	Thus, we suggest thermoresponsive agar microspheres as an alternative separation media for all-aqueous bioseparations.					
30556278	2	42	theme	agar	332:335	arg1	microspheres					337:348	the agar microspheres	328:348	the agar microspheres via atom transfer radical polymerization	328:389	The thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-lauryl methacrylate-co-acrylamide), was grafted on the agar microspheres via atom transfer radical polymerization.					
30556278	3	43	theme	X-ray	579:583	arg1	spectroscopy					599:610	X-ray photoelectron spectroscopy	579:610	X-ray photoelectron spectroscopy	579:610	The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy.					
30556278	4	44	dep	hydrophobicity	723:736	arg1	the					719:721	the	719:721	the	719:721	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	3	45	theme	photoelectron	585:597	arg1	spectroscopy					599:610	X-ray photoelectron spectroscopy	579:610	X-ray photoelectron spectroscopy	579:610	The agar microspheres grafted with copolymers were characterized by light microphotography, elemental analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray photoelectron spectroscopy.					
30556278	4	46	theme	adsorption	742:751	arg1	capacity					753:760	adsorption capacity	742:760	adsorption capacity	742:760	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	0	47	theme	all-aqueous	72:82	arg1	bioseparations					84:97	all-aqueous bioseparations	72:97	all-aqueous bioseparations	72:97	Thermoresponsive hydrophobic copolymer grafted on agar microspheres for all-aqueous bioseparations.					
30556278	4	48	theme	chain	617:621	arg1	lengths					623:629	chain lengths	617:629	chain lengths	617:629	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
30556278	1	49	theme	alkaline	183:190	arg1	condition					192:200	alkaline condition	183:200	alkaline condition	183:200	Agar microspheres were prepared by water-oil emulsification and cross-linked under alkaline condition.					
30556278	4	50	dep	lengths	623:629	arg1	The					613:615	The	613:615	The	613:615	The chain lengths and hydrophobic monomer ratio of the grafting linear polymer had significant effects on the hydrophobicity and adsorption capacity of agar microspheres at different temperatures.					
29948664	6	0	theme	sedentary	857:865	arg1	fibers					847:852	muscle fibers	840:852	muscle fibers of sedentary	840:865	Specific force production was ~ 10 to 25% lower in muscle fibers of sedentary compared to active rats, and excitability of skinned fibers was decreased.					
29948664	9	1	from	changes	1230:1236	arg1	content					1241:1247	content	1241:1247	content of the calcium handling proteins calsequestrin and SERCA	1241:1304	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	6	2	theme	fibers	920:925	arg1	excitability					896:907	excitability	896:907	excitability of skinned fibers	896:925	Specific force production was ~ 10 to 25% lower in muscle fibers of sedentary compared to active rats, and excitability of skinned fibers was decreased.					
29948664	9	3	theme	handling	1264:1271	arg1	proteins					1273:1280	calcium handling proteins	1256:1280	the calcium handling proteins calsequestrin and SERCA	1252:1304	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	3	theme	handling	1264:1271	arg1	SERCA					1300:1304	SERCA	1300:1304	SERCA	1300:1304	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	3	theme	handling	1264:1271	arg1	calsequestrin					1282:1294	calsequestrin	1282:1294	calsequestrin	1282:1294	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	0	4	theme	active	83:88	arg1	rats					90:93	sedentary and active rats	69:93	sedentary and active rats	69:93	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats.					
29948664	6	5	theme	Specific	789:796	arg1	production					804:813	Specific force production	789:813	Specific force production	789:813	Specific force production was ~ 10 to 25% lower in muscle fibers of sedentary compared to active rats, and excitability of skinned fibers was decreased.					
29948664	8	6	theme	in	1195:1196	arg1	%					1206:1206	GAPDH content in SOL ~ 35%	1181:1206	GAPDH content in SOL ~ 35%	1181:1206	Na+, K+-ATPase α1 subunit density was ~ 20% lower in both EDL and SOL in sedentary rats, and GAPDH content in SOL ~ 35% higher.					
29948664	9	7	from	%	1380:1380	arg1	EDL					1385:1387	EDL	1385:1387	EDL	1385:1387	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	7	from	%	1380:1380	arg1	SOL					1400:1402	SOL	1400:1402	SOL	1400:1402	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	8	theme	proteins	1273:1280	arg1	content					1241:1247	content	1241:1247	content of the calcium handling proteins calsequestrin and SERCA	1241:1304	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	4	9	theme	calcium	485:491	arg1	sensitivity					493:503	calcium sensitivity	485:503	calcium sensitivity of the contractile apparatus	485:532	When measured within 1-2 h post-running, calcium sensitivity of the contractile apparatus was increased, but only in type II fibers.					
29948664	7	10	theme	sedentary	1035:1043	arg1	rats					1045:1048	sedentary rats	1035:1048	sedentary rats	1035:1048	Muscle glycogen content was ~ 30% lower and glycogen synthase content ~ 50% higher in SOL of sedentary rats, and in EDL glycogenin was 30% lower.					
29948664	2	11	theme	wheel	313:317	arg1	running					319:325	voluntary wheel running	303:325	voluntary wheel running for either 6 or 12 weeks	303:350	Changes in muscle characteristics with chronic inactivity were investigated by comparing sedentary rats with rats undertaking voluntary wheel running for either 6 or 12 weeks.					
29948664	9	12	dep	proteins	1273:1280	arg1	proteins					1273:1280	calcium handling proteins	1256:1280	the calcium handling proteins calsequestrin and SERCA	1252:1304	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	12	dep	proteins	1273:1280	arg1	SERCA					1300:1304	SERCA	1300:1304	SERCA	1300:1304	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	12	dep	proteins	1273:1280	arg1	calsequestrin					1282:1294	calsequestrin	1282:1294	calsequestrin	1282:1294	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	13	dep	increased	1347:1355	arg1	%					1395:1395	60%	1393:1395	60% in SOL	1393:1402	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	13	dep	increased	1347:1355	arg1	%					1380:1380	~ 20%	1376:1380	~ 20% in EDL	1376:1387	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	6	14	theme	muscle	840:845	arg1	fibers					847:852	muscle fibers	840:852	muscle fibers of sedentary	840:865	Specific force production was ~ 10 to 25% lower in muscle fibers of sedentary compared to active rats, and excitability of skinned fibers was decreased.					
29948664	7	15	theme	rats	1045:1048	arg1	SOL					1028:1030	SOL	1028:1030	SOL of sedentary rats	1028:1048	Muscle glycogen content was ~ 30% lower and glycogen synthase content ~ 50% higher in SOL of sedentary rats, and in EDL glycogenin was 30% lower.					
29948664	2	16	theme	voluntary	303:311	arg1	running					319:325	voluntary wheel running	303:325	voluntary wheel running for either 6 or 12 weeks	303:350	Changes in muscle characteristics with chronic inactivity were investigated by comparing sedentary rats with rats undertaking voluntary wheel running for either 6 or 12 weeks.					
29948664	4	17	theme	type	561:564	arg1	fibers					569:574	type II fibers	561:574	type II fibers	561:574	When measured within 1-2 h post-running, calcium sensitivity of the contractile apparatus was increased, but only in type II fibers.					
29948664	5	18	theme	running	754:760	arg1	24 h					762:765	ceased running 24 h	747:765	ceased running 24 h prior to experiments	747:786	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	10	19	theme	Ca2+	1497:1500	arg1	sensitivity					1502:1512	Ca2+ sensitivity	1497:1512	Ca2+ sensitivity	1497:1512	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	10	20	theme	acute	1461:1465	arg1	increase					1485:1492	an acute oxidation-induced increase	1458:1492	an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers	1458:1530	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	3	21	theme	I	419:419	arg1	fibers					421:426	predominantly type I fibers	400:426	predominantly type I fibers	400:426	EDL (type II fibers) and soleus (SOL) muscles (predominantly type I fibers) were examined.					
29948664	3	21	theme	I	419:419	arg1	muscles					391:397	soleus (SOL) muscles	378:397	soleus (SOL) muscles (predominantly type I fibers)	378:427	EDL (type II fibers) and soleus (SOL) muscles (predominantly type I fibers) were examined.					
29948664	3	22	theme	type	358:361	arg1	EDL					353:355	EDL	353:355	EDL (type II fibers)	353:372	EDL (type II fibers) and soleus (SOL) muscles (predominantly type I fibers) were examined.					
29948664	3	22	theme	type	358:361	arg1	fibers					366:371	type II fibers	358:371	type II fibers	358:371	EDL (type II fibers) and soleus (SOL) muscles (predominantly type I fibers) were examined.					
29948664	4	23	theme	1-2 h	465:469	arg1	post-running					471:482	1-2 h post-running	465:482	1-2 h post-running	465:482	When measured within 1-2 h post-running, calcium sensitivity of the contractile apparatus was increased, but only in type II fibers.					
29948664	5	24	theme	prior	767:771	arg1	24 h					762:765	ceased running 24 h	747:765	ceased running 24 h prior to experiments	747:786	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	6	25	theme	active	879:884	arg1	rats					886:889	active rats	879:889	active rats	879:889	Specific force production was ~ 10 to 25% lower in muscle fibers of sedentary compared to active rats, and excitability of skinned fibers was decreased.					
29948664	3	26	theme	soleus	378:383	arg1	fibers					421:426	predominantly type I fibers	400:426	predominantly type I fibers	400:426	EDL (type II fibers) and soleus (SOL) muscles (predominantly type I fibers) were examined.					
29948664	3	26	theme	soleus	378:383	arg1	muscles					391:397	soleus (SOL) muscles	378:397	soleus (SOL) muscles (predominantly type I fibers)	378:427	EDL (type II fibers) and soleus (SOL) muscles (predominantly type I fibers) were examined.					
29948664	2	27	from	Changes	177:183	arg1	characteristics					195:209	muscle characteristics	188:209	muscle characteristics with chronic inactivity	188:233	Changes in muscle characteristics with chronic inactivity were investigated by comparing sedentary rats with rats undertaking voluntary wheel running for either 6 or 12 weeks.					
29948664	0	28	theme	Physiological	0:12	arg1	characteristics					30:44	Physiological and biochemical characteristics	0:44	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats	0:93	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats.					
29948664	6	29	theme	skinned	912:918	arg1	fibers					920:925	skinned fibers	912:925	skinned fibers	912:925	Specific force production was ~ 10 to 25% lower in muscle fibers of sedentary compared to active rats, and excitability of skinned fibers was decreased.					
29948664	5	30	from	fibers	721:726	arg1	absent					711:716	absent	711:716	absent	711:716	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	1	31	theme	Laboratory	96:105	arg1	rats					107:110	Laboratory rats	96:110	Laboratory rats	96:110	Laboratory rats are sedentary if housed in conditions where activity is limited.					
29948664	3	32	theme	type	414:417	arg1	fibers					421:426	predominantly type I fibers	400:426	predominantly type I fibers	400:426	EDL (type II fibers) and soleus (SOL) muscles (predominantly type I fibers) were examined.					
29948664	3	32	theme	type	414:417	arg1	muscles					391:397	soleus (SOL) muscles	378:397	soleus (SOL) muscles (predominantly type I fibers)	378:427	EDL (type II fibers) and soleus (SOL) muscles (predominantly type I fibers) were examined.					
29948664	10	33	theme	skeletal	1580:1587	arg1	muscle					1589:1594	skeletal muscle characteristics and biochemical processes	1580:1636	muscle	1589:1594	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	0	34	theme	biochemical	18:28	arg1	characteristics					30:44	Physiological and biochemical characteristics	0:44	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats	0:93	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats.					
29948664	7	35	theme	glycogen	986:993	arg1	%					1016:1016	glycogen synthase content ~ 50%	986:1016	glycogen synthase content ~ 50%	986:1016	Muscle glycogen content was ~ 30% lower and glycogen synthase content ~ 50% higher in SOL of sedentary rats, and in EDL glycogenin was 30% lower.					
29948664	10	36	theme	substantial	1557:1567	arg1	changes					1569:1575	substantial changes	1557:1575	substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats	1557:1654	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	8	37	theme	content	1187:1193	arg1	%					1206:1206	GAPDH content in SOL ~ 35%	1181:1206	GAPDH content in SOL ~ 35%	1181:1206	Na+, K+-ATPase α1 subunit density was ~ 20% lower in both EDL and SOL in sedentary rats, and GAPDH content in SOL ~ 35% higher.					
29948664	0	38	theme	skeletal	49:56	arg1	muscles					58:64	skeletal muscles	49:64	skeletal muscles	49:64	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats.					
29948664	10	39	dep	muscle	1589:1594	arg1	characteristics					1596:1610	characteristics	1596:1610	characteristics	1596:1610	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	5	40	theme	contractile	680:690	arg1	apparatus					692:700	the contractile apparatus	676:700	the contractile apparatus	676:700	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	5	41	from	absent	711:716	arg1	fibers					721:726	fibers	721:726	fibers from rats that had ceased running 24 h prior to experiments	721:786	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	9	42	theme	CSQ-like	1326:1333	arg1	protein					1335:1341	CSQ-like protein	1326:1341	CSQ-like protein	1326:1341	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	8	43	theme	subunit	1106:1112	arg1	density					1114:1120	subunit density	1106:1120	subunit density	1106:1120	Na+, K+-ATPase α1 subunit density was ~ 20% lower in both EDL and SOL in sedentary rats, and GAPDH content in SOL ~ 35% higher.					
29948664	5	44	theme	apparatus	692:700	arg1	regulation					662:671	oxidative regulation	652:671	oxidative regulation of the contractile apparatus	652:700	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	9	45	theme	protein	1335:1341	arg1	content					1315:1321	the content	1311:1321	the content of CSQ-like protein	1311:1341	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	7	46	theme	in	1055:1056	arg1	glycogenin					1062:1071	in EDL glycogenin	1055:1071	in EDL glycogenin	1055:1071	Muscle glycogen content was ~ 30% lower and glycogen synthase content ~ 50% higher in SOL of sedentary rats, and in EDL glycogenin was 30% lower.					
29948664	5	47	theme	indicative	638:647	arg1	DTT					633:635	DTT	633:635	DTT	633:635	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	10	48	theme	biochemical	1616:1626	arg1	processes					1628:1636	biochemical processes	1616:1636	biochemical processes	1616:1636	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	7	49	theme	synthase	995:1002	arg1	%					1016:1016	glycogen synthase content ~ 50%	986:1016	glycogen synthase content ~ 50%	986:1016	Muscle glycogen content was ~ 30% lower and glycogen synthase content ~ 50% higher in SOL of sedentary rats, and in EDL glycogenin was 30% lower.					
29948664	0	50	theme	muscles	58:64	arg1	characteristics					30:44	Physiological and biochemical characteristics	0:44	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats	0:93	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats.					
29948664	4	51	theme	apparatus	524:532	arg1	sensitivity					493:503	calcium sensitivity	485:503	calcium sensitivity of the contractile apparatus	485:532	When measured within 1-2 h post-running, calcium sensitivity of the contractile apparatus was increased, but only in type II fibers.					
29948664	2	52	theme	chronic	216:222	arg1	inactivity					224:233	chronic inactivity	216:233	chronic inactivity	216:233	Changes in muscle characteristics with chronic inactivity were investigated by comparing sedentary rats with rats undertaking voluntary wheel running for either 6 or 12 weeks.					
29948664	6	53	theme	force	798:802	arg1	production					804:813	Specific force production	789:813	Specific force production	789:813	Specific force production was ~ 10 to 25% lower in muscle fibers of sedentary compared to active rats, and excitability of skinned fibers was decreased.					
29948664	7	54	theme	Muscle	942:947	arg1	content					958:964	Muscle glycogen content	942:964	Muscle glycogen content	942:964	Muscle glycogen content was ~ 30% lower and glycogen synthase content ~ 50% higher in SOL of sedentary rats, and in EDL glycogenin was 30% lower.					
29948664	8	55	theme	sedentary	1161:1169	arg1	rats					1171:1174	sedentary rats	1161:1174	sedentary rats	1161:1174	Na+, K+-ATPase α1 subunit density was ~ 20% lower in both EDL and SOL in sedentary rats, and GAPDH content in SOL ~ 35% higher.					
29948664	4	56	theme	contractile	512:522	arg1	apparatus					524:532	the contractile apparatus	508:532	the contractile apparatus	508:532	When measured within 1-2 h post-running, calcium sensitivity of the contractile apparatus was increased, but only in type II fibers.					
29948664	5	57	theme	oxidative	652:660	arg1	regulation					662:671	oxidative regulation	652:671	oxidative regulation of the contractile apparatus	652:700	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	5	58	from	rats	733:736	arg1	fibers					721:726	fibers	721:726	fibers from rats that had ceased running 24 h prior to experiments	721:786	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	7	59	theme	EDL	1058:1060	arg1	glycogenin					1062:1071	in EDL glycogenin	1055:1071	in EDL glycogenin	1055:1071	Muscle glycogen content was ~ 30% lower and glycogen synthase content ~ 50% higher in SOL of sedentary rats, and in EDL glycogenin was 30% lower.					
29948664	7	60	theme	glycogen	949:956	arg1	content					958:964	Muscle glycogen content	942:964	Muscle glycogen content	942:964	Muscle glycogen content was ~ 30% lower and glycogen synthase content ~ 50% higher in SOL of sedentary rats, and in EDL glycogenin was 30% lower.					
29948664	10	61	theme	type	1517:1520	arg1	fibers					1525:1530	type II fibers	1517:1530	type II fibers	1517:1530	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	5	62	theme	regulation	662:671	arg1	indicative					638:647	indicative	638:647	indicative	638:647	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	5	63	contain	had	743:745	arg2	24 h					762:765	ceased running 24 h	747:765	ceased running 24 h prior to experiments	747:786	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	5	63	contain	had	743:745	arg1	rats					733:736	rats	733:736	rats that had ceased running 24 h prior to experiments	733:786	This increase disappeared when fibers were treated with DTT, indicative of oxidative regulation of the contractile apparatus, and was absent in fibers from rats that had ceased running 24 h prior to experiments.					
29948664	9	64	theme	active	1360:1365	arg1	rats					1367:1370	active rats	1360:1370	active rats	1360:1370	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	10	65	from	changes	1569:1575	arg1	processes					1628:1636	biochemical processes	1616:1636	biochemical processes	1616:1636	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	10	65	from	changes	1569:1575	arg1	rats					1651:1654	sedentary rats	1641:1654	sedentary rats	1641:1654	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	10	65	from	changes	1569:1575	arg1	muscle					1589:1594	skeletal muscle characteristics and biochemical processes	1580:1636	muscle	1589:1594	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	2	66	theme	muscle	188:193	arg1	characteristics					195:209	muscle characteristics	188:209	muscle characteristics with chronic inactivity	188:233	Changes in muscle characteristics with chronic inactivity were investigated by comparing sedentary rats with rats undertaking voluntary wheel running for either 6 or 12 weeks.					
29948664	2	67	theme	sedentary	266:274	arg1	rats					276:279	sedentary rats	266:279	sedentary rats	266:279	Changes in muscle characteristics with chronic inactivity were investigated by comparing sedentary rats with rats undertaking voluntary wheel running for either 6 or 12 weeks.					
29948664	10	68	from	increase	1485:1492	arg1	fibers					1525:1530	type II fibers	1517:1530	type II fibers	1517:1530	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	10	68	from	increase	1485:1492	arg1	sensitivity					1502:1512	Ca2+ sensitivity	1497:1512	Ca2+ sensitivity	1497:1512	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	2	69	with	characteristics	195:209	arg1	inactivity					224:233	chronic inactivity	216:233	chronic inactivity	216:233	Changes in muscle characteristics with chronic inactivity were investigated by comparing sedentary rats with rats undertaking voluntary wheel running for either 6 or 12 weeks.					
29948664	9	70	from	%	1395:1395	arg1	EDL					1385:1387	EDL	1385:1387	EDL	1385:1387	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	70	from	%	1395:1395	arg1	SOL					1400:1402	SOL	1400:1402	SOL	1400:1402	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	10	71	theme	oxidation-induced	1467:1483	arg1	increase					1485:1492	an acute oxidation-induced increase	1458:1492	an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers	1458:1530	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	0	72	theme	sedentary	69:77	arg1	rats					90:93	sedentary and active rats	69:93	sedentary and active rats	69:93	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats.					
29948664	0	73	from	characteristics	30:44	arg1	rats					90:93	sedentary and active rats	69:93	sedentary and active rats	69:93	Physiological and biochemical characteristics of skeletal muscles in sedentary and active rats.					
29948664	10	74	theme	sedentary	1641:1649	arg1	rats					1651:1654	sedentary rats	1641:1654	sedentary rats	1641:1654	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	6	75	dep	25	827:828	arg1	to					824:825	to	824:825	to	824:825	Specific force production was ~ 10 to 25% lower in muscle fibers of sedentary compared to active rats, and excitability of skinned fibers was decreased.					
29948664	8	76	theme	GAPDH	1181:1185	arg1	%					1206:1206	GAPDH content in SOL ~ 35%	1181:1206	GAPDH content in SOL ~ 35%	1181:1206	Na+, K+-ATPase α1 subunit density was ~ 20% lower in both EDL and SOL in sedentary rats, and GAPDH content in SOL ~ 35% higher.					
29948664	10	77	theme	voluntary	1431:1439	arg1	exercise					1441:1448	voluntary exercise	1431:1448	voluntary exercise	1431:1448	These findings show that voluntary exercise elicits an acute oxidation-induced increase in Ca2+ sensitivity in type II fibers, and also that there are substantial changes in skeletal muscle characteristics and biochemical processes in sedentary rats.					
29948664	9	78	theme	calcium	1256:1262	arg1	proteins					1273:1280	calcium handling proteins	1256:1280	the calcium handling proteins calsequestrin and SERCA	1252:1304	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	78	theme	calcium	1256:1262	arg1	SERCA					1300:1304	SERCA	1300:1304	SERCA	1300:1304	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
29948664	9	78	theme	calcium	1256:1262	arg1	calsequestrin					1282:1294	calsequestrin	1282:1294	calsequestrin	1282:1294	There were no changes in content of the calcium handling proteins calsequestrin and SERCA, but the content of CSQ-like protein was increased in active rats (by ~ 20% in EDL and 60% in SOL).					
30064105	3	0	theme	nitrogen	730:737	arg1	assimilation					755:766	nitrogen and phosphorous assimilation	730:766	assimilation	755:766	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	2	1	theme	molecular	359:367	arg1	characterization					369:384	The molecular characterization	355:384	The molecular characterization of initial biomasses and final products	355:424	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	5	2	theme	ligno-cellulose	1264:1278	arg1	additives					1331:1339	composting additives	1320:1339	composting additives	1320:1339	The results confirm that ligno-cellulose residues may be effectively recycled as composting additives in order to enrich mature compost in aromatic and lignin compounds.					
30064105	5	2	theme	ligno-cellulose	1264:1278	arg1	residues					1280:1287	ligno-cellulose residues	1264:1287	ligno-cellulose residues	1264:1287	The results confirm that ligno-cellulose residues may be effectively recycled as composting additives in order to enrich mature compost in aromatic and lignin compounds.					
30064105	4	3	theme	maize	1183:1187	arg1	roots					1189:1193	maize roots	1183:1193	maize roots	1183:1193	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	6	4	theme	molecular	1440:1448	arg1	characteristics					1450:1464	molecular characteristics	1440:1464	molecular characteristics	1440:1464	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	6	5	theme	agricultural	1636:1647	arg1	practices					1649:1657	sustainable agricultural practices	1624:1657	sustainable agricultural practices	1624:1657	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	1	6	theme	woody	198:202	arg1	residues					133:140	The ligno-cellulose residues	113:140	The ligno-cellulose residues from biorefinery production of bio-ethanol	113:183	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	1	6	theme	woody	198:202	arg1	material					216:223	woody structuring material	198:223	woody structuring material within an on-farm composting system	198:259	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	6	7	theme	ligno-cellulose	1598:1612	arg1	wastes					1614:1619	composted ligno-cellulose wastes	1588:1619	composted ligno-cellulose wastes in sustainable agricultural practices	1588:1657	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	3	8	theme	soluble	823:829	arg1	compounds					839:847	water soluble organic compounds	817:847	water soluble organic compounds	817:847	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	0	9	from	compost	56:62	arg1	Bioactivity					0:10	Bioactivity	0:10	Bioactivity of humic substances	0:30	Bioactivity of humic substances and water extracts from compost made by ligno-cellulose wastes from biorefinery.					
30064105	0	9	from	compost	56:62	arg1	extracts					42:49	water extracts	36:49	water extracts from compost	36:62	Bioactivity of humic substances and water extracts from compost made by ligno-cellulose wastes from biorefinery.					
30064105	4	10	theme	P	1229:1229	arg1	uptake					1231:1236	P uptake	1229:1236	P uptake	1229:1236	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	1	11	theme	ligno-cellulose	117:131	arg1	residues					133:140	The ligno-cellulose residues	113:140	The ligno-cellulose residues from biorefinery production of bio-ethanol	113:183	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	1	11	theme	ligno-cellulose	117:131	arg1	material					216:223	woody structuring material	198:223	woody structuring material within an on-farm composting system	198:259	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	2	12	theme	corresponding	616:628	arg1	extracts					630:637	corresponding extracts	616:637	corresponding extracts	616:637	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	0	13	from	biorefinery	100:110	arg1	wastes					88:93	ligno-cellulose wastes	72:93	ligno-cellulose wastes from biorefinery	72:110	Bioactivity of humic substances and water extracts from compost made by ligno-cellulose wastes from biorefinery.					
30064105	3	14	theme	humic	853:857	arg1	substances					859:868	humic substances	853:868	humic substances	853:868	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	3	15	theme	composts	673:680	arg1	use					650:652	the use	646:652	the use of the stable bulk composts as organic fertilizer	646:702	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	3	16	theme	pot	895:897	arg1	experiments					899:909	pot experiments	895:909	pot experiments	895:909	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	2	17	theme	specific	542:549	arg1	derivatives					560:570	specific phenolic derivatives	542:570	specific phenolic derivatives	542:570	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	1	18	used	used	190:193	arg2	material					216:223	woody structuring material	198:223	woody structuring material within an on-farm composting system	198:259	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	1	18	used	used	190:193	arg2	residues					133:140	The ligno-cellulose residues	113:140	The ligno-cellulose residues from biorefinery production of bio-ethanol	113:183	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	1	19	theme	composted	334:342	arg1	materials					344:352	composted materials	334:352	composted materials	334:352	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	0	20	theme	humic	15:19	arg1	substances					21:30	humic substances	15:30	humic substances	15:30	Bioactivity of humic substances and water extracts from compost made by ligno-cellulose wastes from biorefinery.					
30064105	4	21	from	role	1122:1125	arg1	stimulation					1168:1178	the stimulation	1164:1178	the stimulation of maize roots and shoots	1164:1204	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	1	22	theme	bio-ethanol	173:183	arg1	production					159:168	biorefinery production	147:168	biorefinery production of bio-ethanol	147:183	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	2	23	theme	ligno-cellulose	575:589	arg1	incorporation					525:537	the selective incorporation	511:537	the selective incorporation of specific phenolic derivatives of ligno-cellulose	511:589	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	2	24	theme	derivatives	560:570	arg1	incorporation					525:537	the selective incorporation	511:537	the selective incorporation of specific phenolic derivatives of ligno-cellulose	511:589	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	5	25	theme	lignin	1391:1396	arg1	compounds					1398:1406	aromatic and lignin compounds	1378:1406	aromatic and lignin compounds	1378:1406	The results confirm that ligno-cellulose residues may be effectively recycled as composting additives in order to enrich mature compost in aromatic and lignin compounds.					
30064105	2	26	theme	products	417:424	arg1	characterization					369:384	The molecular characterization	355:384	The molecular characterization of initial biomasses and final products	355:424	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	0	27	theme	water	36:40	arg1	extracts					42:49	water extracts	36:49	water extracts from compost	36:62	Bioactivity of humic substances and water extracts from compost made by ligno-cellulose wastes from biorefinery.					
30064105	4	28	theme	substances	1092:1101	arg1	concentration					1038:1050	concentration	1038:1050	concentration	1038:1050	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	28	theme	substances	1092:1101	arg1	composition					1022:1032	molecular composition	1012:1032	molecular composition	1012:1032	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	6	29	dep	characteristics	1450:1464	arg1	the					1436:1438	the	1436:1438	the	1436:1438	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	2	30	theme	stable	465:470	arg1	compounds					472:480	more stable compounds	460:480	more stable compounds	460:480	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	6	31	theme	materials	1505:1513	arg1	characteristics					1450:1464	molecular characteristics	1440:1464	molecular characteristics	1440:1464	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	6	31	theme	materials	1505:1513	arg1	properties					1481:1490	biological properties	1470:1490	biological properties	1470:1490	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	6	32	from	wastes	1614:1619	arg1	practices					1649:1657	sustainable agricultural practices	1624:1657	sustainable agricultural practices	1624:1657	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	6	32	from	wastes	1614:1619	arg1	derivatives					1571:1581	the most suitable derivatives	1553:1581	the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices	1553:1657	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	4	33	theme	water	1067:1071	arg1	extracts					1073:1080	the applied water extracts	1055:1080	the applied water extracts	1055:1080	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	5	34	theme	aromatic	1378:1385	arg1	compounds					1398:1406	aromatic and lignin compounds	1378:1406	aromatic and lignin compounds	1378:1406	The results confirm that ligno-cellulose residues may be effectively recycled as composting additives in order to enrich mature compost in aromatic and lignin compounds.					
30064105	3	35	from	compounds	839:847	arg1	tests					885:889	germination tests	873:889	germination tests	873:889	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	3	35	from	compounds	839:847	arg1	experiments					899:909	pot experiments	895:909	pot experiments	895:909	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	3	36	theme	organic	685:691	arg1	fertilizer					693:702	organic fertilizer	685:702	organic fertilizer	685:702	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	1	37	from	materials	344:352	arg1	fractions					319:327	bioactive water soluble and humic fractions	285:327	bioactive water soluble and humic fractions from composted materials	285:352	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	3	38	theme	stable	661:666	arg1	composts					673:680	the stable bulk composts	657:680	the stable bulk composts	657:680	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	1	39	theme	on-farm	235:241	arg1	system					254:259	an on-farm composting system	232:259	an on-farm composting system	232:259	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	4	40	from	enhancement	1214:1224	arg1	stimulation					1168:1178	the stimulation	1164:1178	the stimulation of maize roots and shoots	1164:1204	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	41	theme	derivatives	1149:1159	arg1	role					1122:1125	a role	1120:1125	a role of phenols and lignin derivatives in the stimulation of maize roots and shoots	1120:1204	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	41	theme	derivatives	1149:1159	arg1	enhancement					1214:1224	the enhancement	1210:1224	the enhancement of P uptake	1210:1236	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	2	42	theme	initial	389:395	arg1	biomasses					397:405	initial biomasses	389:405	initial biomasses	389:405	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	3	43	from	decrease	718:725	arg1	tissues					777:783	maize tissues	771:783	maize tissues	771:783	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	3	44	theme	assimilation	755:766	arg1	decrease					718:725	a decrease	716:725	a decrease of nitrogen and phosphorous assimilation in maize tissues	716:783	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	4	45	theme	differential	930:941	arg1	related					997:1003	related	997:1003	related	997:1003	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	45	theme	differential	930:941	arg1	responses					943:951	The differential responses	926:951	The differential responses obtained in maize seedlings and plants	926:990	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	46	theme	roots	1189:1193	arg1	stimulation					1168:1178	the stimulation	1164:1178	the stimulation of maize roots and shoots	1164:1204	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	6	47	theme	biological	1470:1479	arg1	properties					1481:1490	biological properties	1470:1490	biological properties	1470:1490	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	4	48	theme	shoots	1199:1204	arg1	stimulation					1168:1178	the stimulation	1164:1178	the stimulation of maize roots and shoots	1164:1204	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	3	49	theme	maize	771:775	arg1	tissues					777:783	maize tissues	771:783	maize tissues	771:783	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	6	50	theme	sustainable	1624:1634	arg1	practices					1649:1657	sustainable agricultural practices	1624:1657	sustainable agricultural practices	1624:1657	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	4	51	dep	composition	1022:1032	arg1	the					1008:1010	the	1008:1010	the	1008:1010	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	1	52	theme	structuring	204:214	arg1	residues					133:140	The ligno-cellulose residues	113:140	The ligno-cellulose residues from biorefinery production of bio-ethanol	113:183	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	1	52	theme	structuring	204:214	arg1	material					216:223	woody structuring material	198:223	woody structuring material within an on-farm composting system	198:259	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	2	53	theme	bulk	599:602	arg1	samples					604:610	bulk samples	599:610	bulk samples	599:610	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	6	54	theme	composted	1588:1596	arg1	wastes					1614:1619	composted ligno-cellulose wastes	1588:1619	composted ligno-cellulose wastes in sustainable agricultural practices	1588:1657	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	4	55	theme	uptake	1231:1236	arg1	role					1122:1125	a role	1120:1125	a role of phenols and lignin derivatives in the stimulation of maize roots and shoots	1120:1204	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	55	theme	uptake	1231:1236	arg1	enhancement					1214:1224	the enhancement	1210:1224	the enhancement of P uptake	1210:1236	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	3	56	theme	organic	831:837	arg1	compounds					839:847	water soluble organic compounds	817:847	water soluble organic compounds	817:847	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	5	57	from	compost	1367:1373	arg1	compounds					1398:1406	aromatic and lignin compounds	1378:1406	aromatic and lignin compounds	1378:1406	The results confirm that ligno-cellulose residues may be effectively recycled as composting additives in order to enrich mature compost in aromatic and lignin compounds.					
30064105	3	58	theme	bulk	668:671	arg1	composts					673:680	the stable bulk composts	657:680	the stable bulk composts	657:680	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	4	59	theme	maize	965:969	arg1	seedlings					971:979	maize seedlings	965:979	maize seedlings	965:979	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	1	60	theme	biorefinery	147:157	arg1	production					159:168	biorefinery production	147:168	biorefinery production of bio-ethanol	147:183	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	0	61	theme	ligno-cellulose	72:86	arg1	wastes					88:93	ligno-cellulose wastes	72:93	ligno-cellulose wastes from biorefinery	72:110	Bioactivity of humic substances and water extracts from compost made by ligno-cellulose wastes from biorefinery.					
30064105	2	62	theme	selective	515:523	arg1	incorporation					525:537	the selective incorporation	511:537	the selective incorporation of specific phenolic derivatives of ligno-cellulose	511:589	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	5	63	theme	composting	1320:1329	arg1	additives					1331:1339	composting additives	1320:1339	composting additives	1320:1339	The results confirm that ligno-cellulose residues may be effectively recycled as composting additives in order to enrich mature compost in aromatic and lignin compounds.					
30064105	5	63	theme	composting	1320:1329	arg1	residues					1280:1287	ligno-cellulose residues	1264:1287	ligno-cellulose residues	1264:1287	The results confirm that ligno-cellulose residues may be effectively recycled as composting additives in order to enrich mature compost in aromatic and lignin compounds.					
30064105	3	64	theme	germination	873:883	arg1	tests					885:889	germination tests	873:889	germination tests	873:889	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	6	65	theme	preliminary	1411:1421	arg1	knowledge					1423:1431	A preliminary knowledge	1409:1431	A preliminary knowledge on the molecular characteristics and biological properties of composted materials	1409:1513	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	6	65	theme	preliminary	1411:1421	arg1	requirement					1531:1541	an essential requirement	1518:1541	an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices	1518:1657	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	1	66	from	production	159:168	arg1	residues					133:140	The ligno-cellulose residues	113:140	The ligno-cellulose residues from biorefinery production of bio-ethanol	113:183	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	1	66	from	production	159:168	arg1	material					216:223	woody structuring material	198:223	woody structuring material within an on-farm composting system	198:259	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	2	67	theme	phenolic	551:558	arg1	derivatives					560:570	specific phenolic derivatives	542:570	specific phenolic derivatives	542:570	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	2	68	theme	final	411:415	arg1	products					417:424	final products	411:424	final products	411:424	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	6	69	theme	essential	1521:1529	arg1	knowledge					1423:1431	A preliminary knowledge	1409:1431	A preliminary knowledge on the molecular characteristics and biological properties of composted materials	1409:1513	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	6	69	theme	essential	1521:1529	arg1	requirement					1531:1541	an essential requirement	1518:1541	an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices	1518:1657	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	6	70	from	knowledge	1423:1431	arg1	characteristics					1450:1464	molecular characteristics	1440:1464	molecular characteristics	1440:1464	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	6	70	from	knowledge	1423:1431	arg1	properties					1481:1490	biological properties	1470:1490	biological properties	1470:1490	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	4	71	theme	humic	1086:1090	arg1	substances					1092:1101	humic substances	1086:1101	humic substances	1086:1101	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	5	72	theme	mature	1360:1365	arg1	compost					1367:1373	mature compost	1360:1373	mature compost in aromatic and lignin compounds	1360:1406	The results confirm that ligno-cellulose residues may be effectively recycled as composting additives in order to enrich mature compost in aromatic and lignin compounds.					
30064105	2	73	theme	biomasses	397:405	arg1	characterization					369:384	The molecular characterization	355:384	The molecular characterization of initial biomasses and final products	355:424	The molecular characterization of initial biomasses and final products revealed a transformation towards more stable compounds during composting and showed the selective incorporation of specific phenolic derivatives of ligno-cellulose in both bulk samples and corresponding extracts.					
30064105	0	74	theme	substances	21:30	arg1	Bioactivity					0:10	Bioactivity	0:10	Bioactivity of humic substances	0:30	Bioactivity of humic substances and water extracts from compost made by ligno-cellulose wastes from biorefinery.					
30064105	0	74	theme	substances	21:30	arg1	extracts					42:49	water extracts	36:49	water extracts from compost	36:62	Bioactivity of humic substances and water extracts from compost made by ligno-cellulose wastes from biorefinery.					
30064105	1	75	theme	bioactive	285:293	arg1	fractions					319:327	bioactive water soluble and humic fractions	285:327	bioactive water soluble and humic fractions from composted materials	285:352	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	6	76	theme	composted	1495:1503	arg1	materials					1505:1513	composted materials	1495:1513	composted materials	1495:1513	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	1	77	theme	soluble	301:307	arg1	fractions					319:327	bioactive water soluble and humic fractions	285:327	bioactive water soluble and humic fractions from composted materials	285:352	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	4	78	theme	applied	1059:1065	arg1	extracts					1073:1080	the applied water extracts	1055:1080	the applied water extracts	1055:1080	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	79	theme	molecular	1012:1020	arg1	composition					1022:1032	molecular composition	1012:1032	molecular composition	1012:1032	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	1	80	theme	humic	313:317	arg1	fractions					319:327	bioactive water soluble and humic fractions	285:327	bioactive water soluble and humic fractions from composted materials	285:352	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	4	81	theme	extracts	1073:1080	arg1	concentration					1038:1050	concentration	1038:1050	concentration	1038:1050	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	81	theme	extracts	1073:1080	arg1	composition					1022:1032	molecular composition	1012:1032	molecular composition	1012:1032	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	82	theme	phenols	1130:1136	arg1	role					1122:1125	a role	1120:1125	a role of phenols and lignin derivatives in the stimulation of maize roots and shoots	1120:1204	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	4	82	theme	phenols	1130:1136	arg1	enhancement					1214:1224	the enhancement	1210:1224	the enhancement of P uptake	1210:1236	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	3	83	from	substances	859:868	arg1	tests					885:889	germination tests	873:889	germination tests	873:889	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	3	83	from	substances	859:868	arg1	experiments					899:909	pot experiments	895:909	pot experiments	895:909	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
30064105	6	84	theme	suitable	1562:1569	arg1	derivatives					1571:1581	the most suitable derivatives	1553:1581	the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices	1553:1657	A preliminary knowledge on the molecular characteristics and biological properties of composted materials is an essential requirement to select the most suitable derivatives from composted ligno-cellulose wastes in sustainable agricultural practices.					
30064105	1	85	theme	composting	243:252	arg1	system					254:259	an on-farm composting system	232:259	an on-farm composting system	232:259	The ligno-cellulose residues from biorefinery production of bio-ethanol were used as woody structuring material within an on-farm composting system, with the aim to obtain bioactive water soluble and humic fractions from composted materials.					
30064105	4	86	theme	lignin	1142:1147	arg1	derivatives					1149:1159	lignin derivatives	1142:1159	lignin derivatives	1142:1159	The differential responses obtained in maize seedlings and plants were related to the molecular composition and concentration of the applied water extracts and humic substances, thus suggesting a role of phenols and lignin derivatives in the stimulation of maize roots and shoots and the enhancement of P uptake.					
30064105	3	87	theme	phosphorous	743:753	arg1	assimilation					755:766	nitrogen and phosphorous assimilation	730:766	assimilation	755:766	While the use of the stable bulk composts as organic fertilizer resulted in a decrease of nitrogen and phosphorous assimilation in maize tissues, a bio-stimulation was shown by water soluble organic compounds and humic substances in germination tests and pot experiments, respectively.					
31000276	0	0	dep	Bifidobacterium	75:89	arg1	longum					91:96	longum	91:96	longum	91:96	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	2	1	theme	microbiota	274:283	arg1	modulation					256:265	modulation	256:265	modulation of gut microbiota	256:283	Exploitation of prebiotics and probiotics for modulation of gut microbiota can lead to promising outcomes.					
31000276	10	2	theme	acid-producing	1860:1873	arg1	bacteria					1875:1882	increasing butyric acid-producing bacteria	1841:1882	increasing butyric acid-producing bacteria	1841:1882	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	3	3	from	lemon	462:466	arg1	pectin					450:455	a citric pectin	441:455	a citric pectin from lemon	441:466	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	2	4	theme	promising	297:305	arg1	outcomes					307:314	promising outcomes	297:314	promising outcomes	297:314	Exploitation of prebiotics and probiotics for modulation of gut microbiota can lead to promising outcomes.					
31000276	1	5	theme	high	181:184	arg1	impact					186:191	high impact	181:191	high impact	181:191	The gut microbiota composition and its metabolites have high impact on human health.					
31000276	8	6	theme	B.	1328:1329	arg1	BB-46					1338:1342	B. longum BB-46	1328:1342	B. longum BB-46	1328:1342	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	0	7	theme	citric	110:115	arg1	pectin					117:122	a citric pectin	108:122	a citric pectin	108:122	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	3	8	theme	Intestinal	539:548	arg1	SHIME®					571:576	SHIME®	571:576	SHIME®	571:576	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	3	8	theme	Intestinal	539:548	arg1	Ecosystem					560:568	Human Intestinal Microbial Ecosystem	533:568	Human Intestinal Microbial Ecosystem (SHIME®)	533:577	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	5	9	theme	relative	782:789	arg1	abundances					791:800	the relative abundances	778:800	the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes	778:906	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	6	10	theme	transverse	1190:1199	arg1	colons					1216:1221	the transverse and descending colons	1186:1221	the transverse and descending colons	1186:1221	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	10	11	dep	B.	1747:1748	arg1	longum					1750:1755	longum	1750:1755	longum	1750:1755	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	11	12	theme	human	2021:2025	arg1	health					2027:2032	human health	2021:2032	human health	2021:2032	These findings indicate that B. longum BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health.					
31000276	5	13	dep	B.	943:944	arg1	longum					946:951	longum	946:951	longum	946:951	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	8	14	dep	bacteria	1392:1399	arg1	bacteria					1392:1399	proteolytic bacteria	1380:1399	proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus	1380:1458	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	8	14	dep	bacteria	1392:1399	arg1	Peptoniphilus					1427:1439	Peptoniphilus	1427:1439	Peptoniphilus	1427:1439	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	8	14	dep	bacteria	1392:1399	arg1	Streptococcus					1446:1458	Streptococcus	1446:1458	Streptococcus	1446:1458	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	8	14	dep	bacteria	1392:1399	arg1	Clostridium					1414:1424	Clostridium	1414:1424	Clostridium	1414:1424	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	8	14	dep	bacteria	1392:1399	arg1	Bacteroides					1401:1411	Bacteroides	1401:1411	Bacteroides	1401:1411	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	6	15	theme	Ruminococcaceae	1160:1174	arg1	family					1176:1181	the Ruminococcaceae family	1156:1181	the Ruminococcaceae family	1156:1181	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	4	16	theme	rRNA	665:668	arg1	sequencing					675:684	the 16S rRNA gene sequencing	657:684	the 16S rRNA gene sequencing	657:684	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	1	17	theme	microbiota	133:142	arg1	composition					144:154	The gut microbiota composition	125:154	The gut microbiota composition	125:154	The gut microbiota composition and its metabolites have high impact on human health.					
31000276	9	18	located	observed	1531:1538	arg2	changes					1514:1520	No significant changes	1499:1520	No significant changes	1499:1520	No significant changes could be observed on NH4+ production by treatment with B. longum BB-46, nor did it increase the amount of SCFAs.					
31000276	9	18	located	observed	1531:1538	arg1	production					1548:1557	NH4+ production	1543:1557	NH4+ production by treatment with B. longum BB-46	1543:1591	No significant changes could be observed on NH4+ production by treatment with B. longum BB-46, nor did it increase the amount of SCFAs.					
31000276	4	19	from	Changes	580:586	arg1	activity					631:638	metabolic activity	621:638	metabolic activity	621:638	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	4	19	from	Changes	580:586	arg1	composition					602:612	microbiota composition	591:612	microbiota composition	591:612	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	11	20	theme	B.	1914:1915	arg1	BB-46					1924:1928	B. longum BB-46	1914:1928	B. longum BB-46	1914:1928	These findings indicate that B. longum BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health.					
31000276	9	21	theme	significant	1502:1512	arg1	changes					1514:1520	No significant changes	1499:1520	No significant changes	1499:1520	No significant changes could be observed on NH4+ production by treatment with B. longum BB-46, nor did it increase the amount of SCFAs.					
31000276	8	22	theme	decreased	1472:1480	arg1	production					1487:1496	decreased NH4+ production	1472:1496	decreased NH4+ production	1472:1496	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	4	23	theme	acids	723:727	arg1	analyses					693:700	analyses	693:700	analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+)	693:760	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	5	24	from	increase	766:773	arg1	abundances					791:800	the relative abundances	778:800	the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes	778:906	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	6	25	dep	B.	1014:1015	arg1	longum					1017:1022	longum	1017:1022	longum	1017:1022	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	3	26	theme	probiotic	365:373	arg1	strain					375:380	the probiotic strain	361:380	the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon	361:466	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	4	27	theme	fatty	717:721	arg1	acids					723:727	short-chain fatty acids	705:727	short-chain fatty acids (SCFAs)	705:735	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	4	27	theme	fatty	717:721	arg1	SCFAs					730:734	SCFAs	730:734	SCFAs	730:734	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	0	28	theme	In	0:1	arg1	modulation					9:18	In vitro modulation	0:18	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.	0:123	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	9	29	theme	NH4+	1543:1546	arg1	production					1548:1557	NH4+ production	1543:1557	NH4+ production by treatment with B. longum BB-46	1543:1591	No significant changes could be observed on NH4+ production by treatment with B. longum BB-46, nor did it increase the amount of SCFAs.					
31000276	2	30	theme	probiotics	241:250	arg1	Exploitation					210:221	Exploitation	210:221	Exploitation of prebiotics and probiotics for modulation of gut microbiota	210:283	Exploitation of prebiotics and probiotics for modulation of gut microbiota can lead to promising outcomes.					
31000276	3	31	theme	BB-46	405:409	arg1	effects					350:356	the effects	346:356	the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®)	346:577	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	1	32	contain	have	176:179	arg1	composition					144:154	The gut microbiota composition	125:154	The gut microbiota composition	125:154	The gut microbiota composition and its metabolites have high impact on human health.					
31000276	1	32	contain	have	176:179	arg2	impact					186:191	high impact	181:191	high impact	181:191	The gut microbiota composition and its metabolites have high impact on human health.					
31000276	1	32	contain	have	176:179	arg1	metabolites					164:174	its metabolites	160:174	its metabolites	160:174	The gut microbiota composition and its metabolites have high impact on human health.					
31000276	0	33	theme	human	23:27	arg1	composition					44:54	human gut microbiota composition	23:54	human gut microbiota composition	23:54	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	5	34	dep	Firmicutes	805:814	arg1	members					832:838	especially the members	817:838	especially the members of Lachnospiraceae and Lactobacillaceae families	817:887	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	0	35	theme	microbiota	33:42	arg1	composition					44:54	human gut microbiota composition	23:54	human gut microbiota composition	23:54	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	6	36	with	Treatment	999:1007	arg1	BB-46					1024:1028	B. longum BB-46	1014:1028	B. longum BB-46	1014:1028	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	6	36	with	Treatment	999:1007	arg1	pectin					1034:1039	pectin	1034:1039	pectin	1034:1039	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	5	37	theme	colon	992:996	arg1	compartments					972:983	all compartments	968:983	all compartments of the colon	968:996	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	7	38	theme	butyrate	1242:1249	arg1	levels					1251:1256	the butyrate levels	1238:1256	the butyrate levels	1238:1256	Concurrently, the butyrate levels increased in these two compartments.					
31000276	10	39	theme	intestinal	1811:1820	arg1	levels					1827:1832	the intestinal NH4+ levels	1807:1832	the intestinal NH4+ levels	1807:1832	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	8	40	theme	proteolytic	1380:1390	arg1	Streptococcus					1446:1458	Streptococcus	1446:1458	Streptococcus	1446:1458	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	8	40	theme	proteolytic	1380:1390	arg1	Peptoniphilus					1427:1439	Peptoniphilus	1427:1439	Peptoniphilus	1427:1439	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	8	40	theme	proteolytic	1380:1390	arg1	bacteria					1392:1399	proteolytic bacteria	1380:1399	proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus	1380:1458	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	8	40	theme	proteolytic	1380:1390	arg1	Clostridium					1414:1424	Clostridium	1414:1424	Clostridium	1414:1424	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	8	40	theme	proteolytic	1380:1390	arg1	Bacteroides					1401:1411	Bacteroides	1401:1411	Bacteroides	1401:1411	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	4	41	theme	16S	661:663	arg1	sequencing					675:684	the 16S rRNA gene sequencing	657:684	the 16S rRNA gene sequencing	657:684	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	3	42	theme	healthy	495:501	arg1	adults					503:508	healthy adults	495:508	healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®)	495:577	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	0	43	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	4	44	theme	microbiota	591:600	arg1	composition					602:612	microbiota composition	591:612	microbiota composition	591:612	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	6	45	from	increase	1055:1062	arg1	colons					1216:1221	the transverse and descending colons	1186:1221	the transverse and descending colons	1186:1221	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	6	45	from	increase	1055:1062	arg1	proportions					1071:1081	the proportions	1067:1081	the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus	1067:1139	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	6	45	from	increase	1055:1062	arg1	family					1176:1181	the Ruminococcaceae family	1156:1181	the Ruminococcaceae family	1156:1181	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	11	46	contain	have	1995:1998	arg1	BB-46					1924:1928	B. longum BB-46	1914:1928	B. longum BB-46	1914:1928	These findings indicate that B. longum BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health.					
31000276	11	46	contain	have	1995:1998	arg2	impact					2011:2016	beneficial impact	2000:2016	beneficial impact	2000:2016	These findings indicate that B. longum BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health.					
31000276	11	47	theme	citric	1974:1979	arg1	pectin					1981:1986	the specific citric pectin	1961:1986	the specific citric pectin	1961:1986	These findings indicate that B. longum BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health.					
31000276	9	48	with	treatment	1562:1570	arg1	BB-46					1587:1591	BB-46	1587:1591	BB-46	1587:1591	No significant changes could be observed on NH4+ production by treatment with B. longum BB-46, nor did it increase the amount of SCFAs.					
31000276	3	49	from	adults	503:508	arg1	microbiota					479:488	the gut microbiota	471:488	the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®)	471:577	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	2	50	theme	gut	270:272	arg1	microbiota					274:283	gut microbiota	270:283	gut microbiota	270:283	Exploitation of prebiotics and probiotics for modulation of gut microbiota can lead to promising outcomes.					
31000276	5	51	theme	Firmicutes	805:814	arg1	abundances					791:800	the relative abundances	778:800	the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes	778:906	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	9	52	theme	SCFAs	1628:1632	arg1	SCFAs					1628:1632	SCFAs	1628:1632	SCFAs	1628:1632	No significant changes could be observed on NH4+ production by treatment with B. longum BB-46, nor did it increase the amount of SCFAs.					
31000276	9	52	theme	SCFAs	1628:1632	arg1	amount					1618:1623	the amount	1614:1623	the amount of SCFAs	1614:1632	No significant changes could be observed on NH4+ production by treatment with B. longum BB-46, nor did it increase the amount of SCFAs.					
31000276	6	53	theme	Lactobacillus	1127:1139	arg1	proportions					1071:1081	the proportions	1067:1081	the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus	1067:1139	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	10	54	theme	butyric	1852:1858	arg1	bacteria					1875:1882	increasing butyric acid-producing bacteria	1841:1882	increasing butyric acid-producing bacteria	1841:1882	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	6	55	theme	descending	1205:1214	arg1	colons					1216:1221	the transverse and descending colons	1186:1221	the transverse and descending colons	1186:1221	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	3	56	theme	Microbial	550:558	arg1	SHIME®					571:576	SHIME®	571:576	SHIME®	571:576	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	3	56	theme	Microbial	550:558	arg1	Ecosystem					560:568	Human Intestinal Microbial Ecosystem	533:568	Human Intestinal Microbial Ecosystem (SHIME®)	533:577	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	3	57	theme	Human	533:537	arg1	SHIME®					571:576	SHIME®	571:576	SHIME®	571:576	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	3	57	theme	Human	533:537	arg1	Ecosystem					560:568	Human Intestinal Microbial Ecosystem	533:568	Human Intestinal Microbial Ecosystem (SHIME®)	533:577	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	11	58	dep	B.	1914:1915	arg1	longum					1917:1922	longum	1917:1922	longum	1917:1922	These findings indicate that B. longum BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health.					
31000276	5	59	dep	Lachnospiraceae	843:857	arg1	families					880:887	families	880:887	families	880:887	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	11	60	theme	beneficial	2000:2009	arg1	impact					2011:2016	beneficial impact	2000:2016	beneficial impact	2000:2016	These findings indicate that B. longum BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health.					
31000276	6	61	theme	Faecalibacterium	1093:1108	arg1	proportions					1071:1081	the proportions	1067:1081	the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus	1067:1139	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	2	62	theme	prebiotics	226:235	arg1	Exploitation					210:221	Exploitation	210:221	Exploitation of prebiotics and probiotics for modulation of gut microbiota	210:283	Exploitation of prebiotics and probiotics for modulation of gut microbiota can lead to promising outcomes.					
31000276	8	63	dep	B.	1328:1329	arg1	longum					1331:1336	longum	1331:1336	longum	1331:1336	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	1	64	theme	human	196:200	arg1	health					202:207	human health	196:207	human health	196:207	The gut microbiota composition and its metabolites have high impact on human health.					
31000276	4	65	theme	metabolic	621:629	arg1	activity					631:638	metabolic activity	621:638	metabolic activity	621:638	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	5	66	with	treatment	928:936	arg1	BB-46					953:957	B. longum BB-46	943:957	B. longum BB-46 alone	943:963	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	3	67	from	effects	350:356	arg1	microbiota					479:488	the gut microbiota	471:488	the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®)	471:577	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	5	68	theme	Lactobacillaceae	863:878	arg1	members					832:838	especially the members	817:838	especially the members of Lachnospiraceae and Lactobacillaceae families	817:887	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	3	69	with	combination	424:434	arg1	pectin					450:455	a citric pectin	441:455	a citric pectin from lemon	441:466	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	10	70	theme	pectin	1767:1772	arg1	efficient					1783:1791	efficient	1783:1791	efficient	1783:1791	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	10	70	theme	pectin	1767:1772	arg1	combination					1732:1742	the combination	1728:1742	the combination of B. longum BB-46 and pectin	1728:1772	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	0	71	theme	metabolites	60:70	arg1	modulation					9:18	In vitro modulation	0:18	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.	0:123	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	10	72	theme	BB-46	1757:1761	arg1	efficient					1783:1791	efficient	1783:1791	efficient	1783:1791	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	10	72	theme	BB-46	1757:1761	arg1	combination					1732:1742	the combination	1728:1742	the combination of B. longum BB-46 and pectin	1728:1772	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	1	73	theme	gut	129:131	arg1	composition					144:154	The gut microbiota composition	125:154	The gut microbiota composition	125:154	The gut microbiota composition and its metabolites have high impact on human health.					
31000276	3	74	theme	Ecosystem	560:568	arg1	Simulator					520:528	the Simulator	516:528	the Simulator of Human Intestinal Microbial Ecosystem (SHIME®)	516:577	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	0	75	theme	Bifidobacterium	75:89	arg1	BB-46					98:102	Bifidobacterium longum BB-46	75:102	Bifidobacterium longum BB-46	75:102	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	5	76	theme	Lachnospiraceae	843:857	arg1	members					832:838	especially the members	817:838	especially the members of Lachnospiraceae and Lactobacillaceae families	817:887	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	8	77	theme	NH4+	1482:1485	arg1	production					1487:1496	decreased NH4+ production	1472:1496	decreased NH4+ production	1472:1496	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	4	78	theme	gene	670:673	arg1	sequencing					675:684	the 16S rRNA gene sequencing	657:684	the 16S rRNA gene sequencing	657:684	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	11	79	theme	specific	1965:1972	arg1	pectin					1981:1986	the specific citric pectin	1961:1986	the specific citric pectin	1961:1986	These findings indicate that B. longum BB-46, especially when combined with the specific citric pectin, might have beneficial impact on human health.					
31000276	3	80	dep	strain	375:380	arg1	longum					398:403	Bifidobacterium longum	382:403	the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon	361:466	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	5	81	theme	B.	943:944	arg1	BB-46					953:957	B. longum BB-46	943:957	B. longum BB-46 alone	943:963	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	5	82	theme	Bacteroidetes	894:906	arg1	abundances					791:800	the relative abundances	778:800	the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes	778:906	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	3	83	theme	strain	375:380	arg1	BB-46					405:409	the probiotic strain Bifidobacterium longum BB-46	361:409	the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon	361:466	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	4	84	theme	short-chain	705:715	arg1	acids					723:727	short-chain fatty acids	705:727	short-chain fatty acids (SCFAs)	705:735	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	4	84	theme	short-chain	705:715	arg1	SCFAs					730:734	SCFAs	730:734	SCFAs	730:734	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	10	85	theme	B.	1747:1748	arg1	BB-46					1757:1761	B. longum BB-46	1747:1761	B. longum BB-46	1747:1761	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	6	86	theme	B.	1014:1015	arg1	BB-46					1024:1028	B. longum BB-46	1014:1028	B. longum BB-46	1014:1028	Treatment with B. longum BB-46 and pectin stimulated an increase in the proportions of genera Faecalibacterium, Eubacterium and Lactobacillus, as well as in the Ruminococcaceae family in the transverse and descending colons.					
31000276	4	87	theme	ammonium	741:748	arg1	NH4+					756:759	NH4+	756:759	NH4+	756:759	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	4	87	theme	ammonium	741:748	arg1	ions					750:753	ammonium ions	741:753	ammonium ions (NH4+)	741:760	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	8	88	theme	BB-46	1338:1342	arg1	combination					1313:1323	the combination	1309:1323	the combination of B. longum BB-46 and pectin	1309:1353	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	0	89	theme	gut	29:31	arg1	composition					44:54	human gut microbiota composition	23:54	human gut microbiota composition	23:54	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	8	90	theme	pectin	1348:1353	arg1	combination					1313:1323	the combination	1309:1323	the combination of B. longum BB-46 and pectin	1309:1353	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31000276	3	91	theme	citric	443:448	arg1	pectin					450:455	a citric pectin	441:455	a citric pectin from lemon	441:466	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	0	92	theme	composition	44:54	arg1	modulation					9:18	In vitro modulation	0:18	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.	0:123	In vitro modulation of human gut microbiota composition and metabolites by Bifidobacterium longum BB-46 and a citric pectin.					
31000276	10	93	theme	NH4+	1822:1825	arg1	levels					1827:1832	the intestinal NH4+ levels	1807:1832	the intestinal NH4+ levels	1807:1832	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	3	94	theme	gut	475:477	arg1	microbiota					479:488	the gut microbiota	471:488	the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®)	471:577	This study aimed to evaluate the effects of the probiotic strain Bifidobacterium longum BB-46 alone and in combination with a citric pectin from lemon on the gut microbiota from healthy adults using the Simulator of Human Intestinal Microbial Ecosystem (SHIME®).					
31000276	5	95	located	observed	912:919	arg2	increase					766:773	An increase	763:773	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes	763:906	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	5	95	located	observed	912:919	arg1	compartments					972:983	all compartments	968:983	all compartments of the colon	968:996	An increase in the relative abundances of Firmicutes (especially the members of Lachnospiraceae and Lactobacillaceae families) and Bacteroidetes was observed during treatment with B. longum BB-46 alone in all compartments of the colon.					
31000276	4	96	theme	ions	750:753	arg1	analyses					693:700	analyses	693:700	analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+)	693:760	Changes in microbiota composition and in metabolic activity were assessed by the 16S rRNA gene sequencing and by analyses of short-chain fatty acids (SCFAs) and ammonium ions (NH4+).					
31000276	10	97	theme	increasing	1841:1850	arg1	bacteria					1875:1882	increasing butyric acid-producing bacteria	1841:1882	increasing butyric acid-producing bacteria	1841:1882	In this study, we observed that although each treatment was able to modulate the microbiota, the combination of B. longum BB-46 and pectin was more efficient in decreasing the intestinal NH4+ levels and in increasing butyric acid-producing bacteria.					
31000276	8	98	theme	bacteria	1392:1399	arg1	abundance					1367:1375	the abundance	1363:1375	the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus	1363:1458	Additionally, the combination of B. longum BB-46 and pectin reduced the abundance of proteolytic bacteria Bacteroides, Clostridium, Peptoniphilus, and Streptococcus, along with decreased NH4+ production.					
31129217	0	0	theme	Arctium	84:90	arg1	lappa					92:96	Arctium lappa	84:96	Arctium lappa	84:96	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	6	1	from	production	1060:1069	arg1	gut					1109:1111	gut	1109:1111	gut	1109:1111	Besides, ALP-1 could significantly enhance the production of short chain fatty acids (SCFAs) in gut.					
31129217	1	2	theme	systemic	256:263	arg1	mice					278:281	lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice	205:281	mice	278:281	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	4	3	from	probiotics	810:819	arg1	stools					828:833	the stools	824:833	the stools of ALP-1 treatment group	824:858	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	2	4	theme	cytokines	371:379	arg1	levels					348:353	the levels	344:353	the levels of inflammatory cytokines	344:379	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	1	5	theme	inflammatory	265:276	arg1	mice					278:281	lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice	205:281	mice	278:281	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	3	6	theme	16 s	623:626	arg1	sequencing					644:653	16 s high-throughput sequencing	623:653	16 s high-throughput sequencing	623:653	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	3	7	theme	treatment	757:765	arg1	group					767:771	ALP-1 treatment group	751:771	ALP-1 treatment group	751:771	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	2	8	dep	cytokines	515:523	arg1	interleukin-6					542:554	interleukin-6	542:554	interleukin-6	542:554	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	8	dep	cytokines	515:523	arg1	cytokines					515:523	down-regulated pro-inflammatory cytokines	483:523	down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α)	483:583	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	8	dep	cytokines	515:523	arg1	interleukin-1β					526:539	interleukin-1β	526:539	interleukin-1β	526:539	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	8	dep	cytokines	515:523	arg1	Factor-α					575:582	Tumor Necrosis Factor-α	560:582	Tumor Necrosis Factor-α	560:582	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	9	theme	Necrosis	566:573	arg1	cytokines					515:523	down-regulated pro-inflammatory cytokines	483:523	down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α)	483:583	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	9	theme	Necrosis	566:573	arg1	Factor-α					575:582	Tumor Necrosis Factor-α	560:582	Tumor Necrosis Factor-α	560:582	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	0	10	from	effects	39:45	arg1	lappa					92:96	Arctium lappa	84:96	Arctium lappa	84:96	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	4	11	from	levels	792:797	arg1	stools					828:833	the stools	824:833	the stools of ALP-1 treatment group	824:858	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	4	12	theme	probiotics	810:819	arg1	levels					792:797	higher levels	785:797	higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium	785:933	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	6	13	theme	acids	1092:1096	arg1	production					1060:1069	the production	1056:1069	the production of short chain fatty acids (SCFAs) in gut	1056:1111	Besides, ALP-1 could significantly enhance the production of short chain fatty acids (SCFAs) in gut.					
31129217	2	14	theme	Tumor	560:564	arg1	cytokines					515:523	down-regulated pro-inflammatory cytokines	483:523	down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α)	483:583	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	14	theme	Tumor	560:564	arg1	Factor-α					575:582	Tumor Necrosis Factor-α	560:582	Tumor Necrosis Factor-α	560:582	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	4	15	theme	several	802:808	arg1	Odoribacter					896:906	Odoribacter	896:906	Odoribacter	896:906	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	4	15	theme	several	802:808	arg1	Phascolarctobacterium					913:933	Phascolarctobacterium	913:933	Phascolarctobacterium	913:933	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	4	15	theme	several	802:808	arg1	Alistipes					885:893	Alistipes	885:893	Alistipes	885:893	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	4	15	theme	several	802:808	arg1	probiotics					810:819	several probiotics	802:819	several probiotics	802:819	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	2	16	theme	mice	409:412	arg1	serum					400:404	serum	400:404	serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α)	400:583	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	16	theme	mice	409:412	arg1	macrophages					384:394	macrophages	384:394	macrophages	384:394	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	3	17	theme	ALP-1	751:755	arg1	group					767:771	ALP-1 treatment group	751:771	ALP-1 treatment group	751:771	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	3	18	from	composition	694:704	arg1	mice					727:730	mice	727:730	mice	727:730	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	1	19	theme	Arctium	169:175	arg1	lappa					177:181	Arctium lappa	169:181	Arctium lappa	169:181	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	0	20	theme	In	0:1	arg1	effects					39:45	In vivo and in vitro anti-inflammatory effects	0:45	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.	0:97	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	0	21	theme	in	12:13	arg1	effects					39:45	In vivo and in vitro anti-inflammatory effects	0:45	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.	0:97	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	4	22	theme	group	854:858	arg1	stools					828:833	the stools	824:833	the stools of ALP-1 treatment group	824:858	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	4	23	theme	higher	785:790	arg1	levels					792:797	higher levels	785:797	higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium	785:933	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	2	24	theme	cytokine	453:460	arg1	interleukin-10					463:476	increased anti-inflammatory cytokine (interleukin-10)	425:477	increased anti-inflammatory cytokine (interleukin-10)	425:477	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	4	25	from	stools	828:833	arg1	levels					792:797	higher levels	785:797	higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium	785:933	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	3	26	from	sequencing	644:653	arg1	data					613:616	our data	609:616	our data from 16 s high-throughput sequencing	609:653	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	2	27	theme	anti-inflammatory	435:451	arg1	interleukin-10					463:476	increased anti-inflammatory cytokine (interleukin-10)	425:477	increased anti-inflammatory cytokine (interleukin-10)	425:477	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	0	28	theme	anti-inflammatory	21:37	arg1	effects					39:45	In vivo and in vitro anti-inflammatory effects	0:45	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.	0:97	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	6	29	theme	fatty	1086:1090	arg1	acids					1092:1096	short chain fatty acids	1074:1096	short chain fatty acids (SCFAs)	1074:1104	Besides, ALP-1 could significantly enhance the production of short chain fatty acids (SCFAs) in gut.					
31129217	6	29	theme	fatty	1086:1090	arg1	SCFAs					1099:1103	SCFAs	1099:1103	SCFAs	1099:1103	Besides, ALP-1 could significantly enhance the production of short chain fatty acids (SCFAs) in gut.					
31129217	4	30	theme	treatment	844:852	arg1	group					854:858	ALP-1 treatment group	838:858	ALP-1 treatment group	838:858	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	2	31	theme	increased	425:433	arg1	interleukin-10					463:476	increased anti-inflammatory cytokine (interleukin-10)	425:477	increased anti-inflammatory cytokine (interleukin-10)	425:477	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	3	32	theme	model	677:681	arg1	group					683:687	LPS model group	673:687	LPS model group	673:687	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	4	33	theme	ALP-1	838:842	arg1	group					854:858	ALP-1 treatment group	838:858	ALP-1 treatment group	838:858	There were higher levels of several probiotics in the stools of ALP-1 treatment group, such as Lactobacillius, Alistipes, Odoribacter, and Phascolarctobacterium.					
31129217	6	34	theme	chain	1080:1084	arg1	acids					1092:1096	short chain fatty acids	1074:1096	short chain fatty acids (SCFAs)	1074:1104	Besides, ALP-1 could significantly enhance the production of short chain fatty acids (SCFAs) in gut.					
31129217	6	34	theme	chain	1080:1084	arg1	SCFAs					1099:1103	SCFAs	1099:1103	SCFAs	1099:1103	Besides, ALP-1 could significantly enhance the production of short chain fatty acids (SCFAs) in gut.					
31129217	2	35	theme	pro-inflammatory	498:513	arg1	interleukin-6					542:554	interleukin-6	542:554	interleukin-6	542:554	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	35	theme	pro-inflammatory	498:513	arg1	Factor-α					575:582	Tumor Necrosis Factor-α	560:582	Tumor Necrosis Factor-α	560:582	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	35	theme	pro-inflammatory	498:513	arg1	interleukin-1β					526:539	interleukin-1β	526:539	interleukin-1β	526:539	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	35	theme	pro-inflammatory	498:513	arg1	cytokines					515:523	down-regulated pro-inflammatory cytokines	483:523	down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α)	483:583	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	1	36	used	used	187:190	arg2	ALP-1					157:161	ALP-1	157:161	ALP-1	157:161	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	1	36	used	used	187:190	arg2	polysaccharide					141:154	the purified water-soluble polysaccharide	114:154	the purified water-soluble polysaccharide (ALP-1) from Arctium lappa	114:181	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	2	37	theme	inflammatory	358:369	arg1	cytokines					371:379	inflammatory cytokines	358:379	inflammatory cytokines	358:379	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	38	theme	down-regulated	483:496	arg1	interleukin-6					542:554	interleukin-6	542:554	interleukin-6	542:554	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	38	theme	down-regulated	483:496	arg1	Factor-α					575:582	Tumor Necrosis Factor-α	560:582	Tumor Necrosis Factor-α	560:582	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	38	theme	down-regulated	483:496	arg1	interleukin-1β					526:539	interleukin-1β	526:539	interleukin-1β	526:539	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	2	38	theme	down-regulated	483:496	arg1	cytokines					515:523	down-regulated pro-inflammatory cytokines	483:523	down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α)	483:583	Our results showed that ALP-1 could effectively accommodate the levels of inflammatory cytokines in macrophages and serum of mice, including increased anti-inflammatory cytokine (interleukin-10) and down-regulated pro-inflammatory cytokines (interleukin-1β, interleukin-6 and Tumor Necrosis Factor-α).					
31129217	3	39	theme	gut	709:711	arg1	microbiota					713:722	gut microbiota	709:722	gut microbiota	709:722	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	3	40	theme	high-throughput	628:642	arg1	sequencing					644:653	16 s high-throughput sequencing	623:653	16 s high-throughput sequencing	623:653	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	5	41	theme	symbiotic	952:960	arg1	bacteria					962:969	symbiotic bacteria	952:969	symbiotic bacteria like Bacteroides	952:986	Simultaneously, symbiotic bacteria like Bacteroides were inhibited by ALP-1.					
31129217	3	42	theme	microbiota	713:722	arg1	composition					694:704	the composition	690:704	the composition of gut microbiota in mice	690:730	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	0	43	dep	in	12:13	arg1	vitro					15:19	vitro	15:19	vitro	15:19	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	1	44	theme	lipopolysaccharide-induced	205:230	arg1	macrophage					241:250	lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice	205:281	macrophage	241:250	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	3	45	theme	LPS	673:675	arg1	group					683:687	LPS model group	673:687	LPS model group	673:687	Moreover, according to our data from 16 s high-throughput sequencing, as compared with LPS model group, the composition of gut microbiota in mice was ameliorated in ALP-1 treatment group.					
31129217	1	46	theme	purified	118:125	arg1	polysaccharide					141:154	the purified water-soluble polysaccharide	114:154	the purified water-soluble polysaccharide (ALP-1) from Arctium lappa	114:181	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	1	46	theme	purified	118:125	arg1	ALP-1					157:161	ALP-1	157:161	ALP-1	157:161	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	1	47	theme	RAW264.7	232:239	arg1	macrophage					241:250	lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice	205:281	macrophage	241:250	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	0	48	theme	polysaccharide	64:77	arg1	effects					39:45	In vivo and in vitro anti-inflammatory effects	0:45	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.	0:97	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	0	49	from	lappa	92:96	arg1	effects					39:45	In vivo and in vitro anti-inflammatory effects	0:45	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.	0:97	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	0	49	from	lappa	92:96	arg1	polysaccharide					64:77	water-soluble polysaccharide	50:77	water-soluble polysaccharide from Arctium lappa	50:96	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	1	50	from	lappa	177:181	arg1	polysaccharide					141:154	the purified water-soluble polysaccharide	114:154	the purified water-soluble polysaccharide (ALP-1) from Arctium lappa	114:181	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	1	50	from	lappa	177:181	arg1	ALP-1					157:161	ALP-1	157:161	ALP-1	157:161	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	1	51	theme	water-soluble	127:139	arg1	polysaccharide					141:154	the purified water-soluble polysaccharide	114:154	the purified water-soluble polysaccharide (ALP-1) from Arctium lappa	114:181	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	1	51	theme	water-soluble	127:139	arg1	ALP-1					157:161	ALP-1	157:161	ALP-1	157:161	In this study, the purified water-soluble polysaccharide (ALP-1) from Arctium lappa was used to intervene lipopolysaccharide-induced RAW264.7 macrophage and systemic inflammatory mice.					
31129217	0	52	theme	water-soluble	50:62	arg1	polysaccharide					64:77	water-soluble polysaccharide	50:77	water-soluble polysaccharide from Arctium lappa	50:96	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	0	53	dep	In	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa.					
31129217	6	54	theme	short	1074:1078	arg1	acids					1092:1096	short chain fatty acids	1074:1096	short chain fatty acids (SCFAs)	1074:1104	Besides, ALP-1 could significantly enhance the production of short chain fatty acids (SCFAs) in gut.					
31129217	6	54	theme	short	1074:1078	arg1	SCFAs					1099:1103	SCFAs	1099:1103	SCFAs	1099:1103	Besides, ALP-1 could significantly enhance the production of short chain fatty acids (SCFAs) in gut.					
30265981	0	0	theme	imprinted	79:87	arg1	carbon					130:135	molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	67:135	molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	67:135	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	5	1	theme	netlike	791:797	arg1	morphology					799:808	netlike morphology	791:808	netlike morphology	791:808	The SA-MWCNTs composite with netlike morphology demonstrated high conductivity and electrocatalytic activity.					
30265981	10	2	theme	human	1576:1580	arg1	samples					1589:1595	human plasma samples	1576:1595	human plasma samples	1576:1595	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	4	3	theme	glassy	731:736	arg1	electrode					745:753	glassy carbon electrode	731:753	glassy carbon electrode (GCE)	731:759	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	4	3	theme	glassy	731:736	arg1	GCE					756:758	GCE	756:758	GCE	756:758	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	1	4	theme	imprinted	274:282	arg1	film					294:297	a novel molecularly imprinted composite film	254:297	a novel molecularly imprinted composite film using the mathematical modeling	254:329	This paper reports on the synthesis of a novel molecularly imprinted composite film using the mathematical modeling.					
30265981	6	5	theme	sensitive	975:983	arg1	peak					992:995	a sensitive anodic peak	973:995	a sensitive anodic peak in 1170 mV	973:1006	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	9	6	theme	added	1355:1359	arg1	standards					1361:1369	the added standards	1351:1369	the added standards	1351:1369	Regarding the added standards, the recoveries were values between 93.4-105%.					
30265981	10	7	theme	theophylline	1524:1535	arg1	tablet					1537:1542	theophylline tablet	1524:1542	theophylline tablet	1524:1542	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	6	8	theme	Cyclic	872:877	arg1	voltammogram					879:890	Cyclic voltammogram	872:890	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline	872:964	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	10	9	theme	theophylline	1490:1501	arg1	determination					1473:1485	the determination	1469:1485	the determination of theophylline	1469:1501	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	8	10	theme	0.01-60.0 μM	1296:1307	arg1	range					1287:1291	the range	1283:1291	the range of 0.01-60.0 μM with detection limit of 3.2 nM	1283:1338	Theophylline response was linearly within the range of 0.01-60.0 μM with detection limit of 3.2 nM.					
30265981	1	11	theme	composite	284:292	arg1	film					294:297	a novel molecularly imprinted composite film	254:297	a novel molecularly imprinted composite film using the mathematical modeling	254:329	This paper reports on the synthesis of a novel molecularly imprinted composite film using the mathematical modeling.					
30265981	0	12	theme	alginate-multiwalled	109:128	arg1	carbon					130:135	molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	67:135	molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	67:135	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	7	13	theme	target	1158:1163	arg1	theophylline					1165:1176	target theophylline	1158:1176	target theophylline	1158:1176	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	1	14	theme	film	294:297	arg1	synthesis					241:249	the synthesis	237:249	the synthesis of a novel molecularly imprinted composite film using the mathematical modeling	237:329	This paper reports on the synthesis of a novel molecularly imprinted composite film using the mathematical modeling.					
30265981	0	15	theme	polyarginine-sodium	89:107	arg1	carbon					130:135	molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	67:135	molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	67:135	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	6	16	theme	theophylline	953:964	arg1	presence					941:948	the presence	937:948	the presence of theophylline	937:964	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	7	17	theme	theophylline	1165:1176	arg1	behavior					1146:1153	the response and electrochemical behavior	1113:1153	behavior	1146:1153	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	10	18	theme	electrode	1439:1447	arg1	satisfactory					1453:1464	satisfactory	1453:1464	satisfactory	1453:1464	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	10	18	theme	electrode	1439:1447	arg1	function					1422:1429	The function	1418:1429	The function of this electrode	1418:1447	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	4	19	theme	electrode	526:534	arg1	modification					510:521	The modification	506:521	The modification of electrode	506:534	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	6	20	from	voltammogram	879:890	arg1	presence					941:948	the presence	937:948	the presence of theophylline	937:964	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	8	21	theme	detection	1314:1322	arg1	limit					1324:1328	detection limit	1314:1328	detection limit of 3.2 nM	1314:1338	Theophylline response was linearly within the range of 0.01-60.0 μM with detection limit of 3.2 nM.					
30265981	3	22	theme	optimum	466:472	arg1	condition					474:482	optimum condition	466:482	optimum condition	466:482	The ratio of monomer to template in optimum condition was obtained to be 4.					
30265981	0	23	theme	carbon	130:135	arg1	preparation					52:62	facile preparation	45:62	facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	45:135	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	4	24	theme	arginine	619:626	arg1	electropolymerization					594:614	the electropolymerization	590:614	the electropolymerization of arginine	590:626	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	2	25	used	used	356:359	arg2	composite					337:345	This composite	332:345	This composite	332:345	This composite was then used in the electrode modification for the determination of theophylline.					
30265981	7	26	theme	electrode	1230:1238	arg1	surface					1206:1212	the surface	1202:1212	the surface of the modified electrode	1202:1238	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	7	27	dep	investigation	1054:1066	arg1	The					1050:1052	The	1050:1052	The	1050:1052	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	6	28	theme	anodic	985:990	arg1	peak					992:995	a sensitive anodic peak	973:995	a sensitive anodic peak in 1170 mV	973:1006	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	5	29	theme	electrocatalytic	845:860	arg1	activity					862:869	electrocatalytic activity	845:869	electrocatalytic activity	845:869	The SA-MWCNTs composite with netlike morphology demonstrated high conductivity and electrocatalytic activity.					
30265981	7	30	theme	effective	1092:1100	arg1	factors					1102:1108	the effective factors	1088:1108	the effective factors	1088:1108	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	6	31	from	peak	992:995	arg1	1170 mV					1000:1006	1170 mV	1000:1006	1170 mV	1000:1006	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	2	32	theme	theophylline	416:427	arg1	determination					399:411	the determination	395:411	the determination of theophylline	395:427	This composite was then used in the electrode modification for the determination of theophylline.					
30265981	0	33	theme	Computational	0:12	arg1	design					14:19	Computational design	0:19	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	0:135	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	6	34	theme	phosphate	1030:1038	arg1	solution					1018:1025	buffer solution	1011:1025	buffer solution of phosphate (pH 7.0)	1011:1047	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	6	34	theme	phosphate	1030:1038	arg1	pH					1041:1042	pH 7.0	1041:1046	pH 7.0	1041:1046	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	7	35	theme	electrochemical	1130:1144	arg1	behavior					1146:1153	the response and electrochemical behavior	1113:1153	behavior	1146:1153	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	10	36	theme	real	1506:1509	arg1	samples					1511:1517	real samples	1506:1517	real samples like theophylline tablet, theophylline oral solution and human plasma samples	1506:1595	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	1	37	theme	mathematical	309:320	arg1	modeling					322:329	the mathematical modeling	305:329	the mathematical modeling	305:329	This paper reports on the synthesis of a novel molecularly imprinted composite film using the mathematical modeling.					
30265981	7	38	theme	factors	1102:1108	arg1	optimization					1072:1083	optimization	1072:1083	optimization	1072:1083	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	7	38	theme	factors	1102:1108	arg1	investigation					1054:1066	investigation	1054:1066	investigation	1054:1066	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	0	39	theme	composite	147:155	arg1	film					157:160	composite film	147:160	composite film on glassy carbon electrode for theophylline sensing	147:212	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	10	40	from	determination	1473:1485	arg1	satisfactory					1453:1464	satisfactory	1453:1464	satisfactory	1453:1464	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	10	40	from	determination	1473:1485	arg1	function					1422:1429	The function	1418:1429	The function of this electrode	1418:1447	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	4	41	theme	carbon	676:681	arg1	SA-MWCNTs					694:702	SA-MWCNTs	694:702	SA-MWCNTs	694:702	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	4	41	theme	carbon	676:681	arg1	nanotubes					683:691	sodium alginate/multiwalled carbon nanotubes	648:691	sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs)	648:703	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	3	42	theme	monomer	443:449	arg1	4					503:503	4	503:503	4	503:503	The ratio of monomer to template in optimum condition was obtained to be 4.					
30265981	3	42	theme	monomer	443:449	arg1	ratio					434:438	The ratio	430:438	The ratio of monomer to template in optimum condition	430:482	The ratio of monomer to template in optimum condition was obtained to be 4.					
30265981	6	43	theme	buffer	1011:1016	arg1	solution					1018:1025	buffer solution	1011:1025	buffer solution of phosphate (pH 7.0)	1011:1047	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	6	43	theme	buffer	1011:1016	arg1	pH					1041:1042	pH 7.0	1041:1046	pH 7.0	1041:1046	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	0	44	theme	glassy	165:170	arg1	electrode					179:187	glassy carbon electrode	165:187	glassy carbon electrode for theophylline sensing	165:212	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	10	45	theme	oral	1558:1561	arg1	solution					1563:1570	theophylline oral solution	1545:1570	theophylline oral solution	1545:1570	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	7	46	from	investigation	1054:1066	arg1	behavior					1146:1153	the response and electrochemical behavior	1113:1153	behavior	1146:1153	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	0	47	theme	green	26:30	arg1	approach					32:39	a green approach	24:39	a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	24:135	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	5	48	theme	high	823:826	arg1	conductivity					828:839	high conductivity	823:839	high conductivity	823:839	The SA-MWCNTs composite with netlike morphology demonstrated high conductivity and electrocatalytic activity.					
30265981	0	49	from	film	157:160	arg1	electrode					179:187	glassy carbon electrode	165:187	glassy carbon electrode for theophylline sensing	165:212	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	8	50	theme	Theophylline	1241:1252	arg1	response					1254:1261	Theophylline response	1241:1261	Theophylline response	1241:1261	Theophylline response was linearly within the range of 0.01-60.0 μM with detection limit of 3.2 nM.					
30265981	0	51	theme	facile	45:50	arg1	preparation					52:62	facile preparation	45:62	facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon	45:135	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	6	52	from	presence	941:948	arg1	voltammogram					879:890	Cyclic voltammogram	872:890	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline	872:964	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	4	53	theme	theophylline	569:580	arg1	presence					557:564	the presence	553:564	the presence of theophylline through the electropolymerization of arginine	553:626	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	4	54	dep	nanotubes	683:691	arg1	the					631:633	the	631:633	the	631:633	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	4	54	dep	nanotubes	683:691	arg1	composite					635:643	composite	635:643	composite	635:643	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	10	55	theme	plasma	1582:1587	arg1	samples					1589:1595	human plasma samples	1576:1595	human plasma samples	1576:1595	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	2	56	theme	electrode	368:376	arg1	modification					378:389	the electrode modification	364:389	the electrode modification	364:389	This composite was then used in the electrode modification for the determination of theophylline.					
30265981	10	57	theme	theophylline	1545:1556	arg1	solution					1563:1570	theophylline oral solution	1545:1570	theophylline oral solution	1545:1570	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	0	58	theme	carbon	172:177	arg1	electrode					179:187	glassy carbon electrode	165:187	glassy carbon electrode for theophylline sensing	165:212	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	6	59	theme	electrode	904:912	arg1	voltammogram					879:890	Cyclic voltammogram	872:890	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline	872:964	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	4	60	theme	carbon	738:743	arg1	electrode					745:753	glassy carbon electrode	731:753	glassy carbon electrode (GCE)	731:759	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	4	60	theme	carbon	738:743	arg1	GCE					756:758	GCE	756:758	GCE	756:758	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	0	61	theme	theophylline	193:204	arg1	sensing					206:212	theophylline sensing	193:212	theophylline sensing	193:212	Computational design as a green approach for facile preparation of molecularly imprinted polyarginine-sodium alginate-multiwalled carbon nanotubes composite film on glassy carbon electrode for theophylline sensing.					
30265981	6	62	theme	modified	895:902	arg1	MIP/SA-MWCNTs/GCE					915:931	MIP/SA-MWCNTs/GCE	915:931	MIP/SA-MWCNTs/GCE	915:931	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	6	62	theme	modified	895:902	arg1	electrode					904:912	modified electrode	895:912	modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline	895:964	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	10	63	from	satisfactory	1453:1464	arg1	samples					1511:1517	real samples	1506:1517	real samples like theophylline tablet, theophylline oral solution and human plasma samples	1506:1595	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	10	63	from	satisfactory	1453:1464	arg1	determination					1473:1485	the determination	1469:1485	the determination of theophylline	1469:1501	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	7	64	from	optimization	1072:1083	arg1	behavior					1146:1153	the response and electrochemical behavior	1113:1153	behavior	1146:1153	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	7	65	theme	response	1117:1124	arg1	behavior					1146:1153	the response and electrochemical behavior	1113:1153	behavior	1146:1153	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	8	66	with	range	1287:1291	arg1	limit					1324:1328	detection limit	1314:1328	detection limit of 3.2 nM	1314:1338	Theophylline response was linearly within the range of 0.01-60.0 μM with detection limit of 3.2 nM.					
30265981	5	67	theme	composite	776:784	arg1	SA-MWCNTs					766:774	The SA-MWCNTs	762:774	The SA-MWCNTs composite with netlike morphology	762:808	The SA-MWCNTs composite with netlike morphology demonstrated high conductivity and electrocatalytic activity.					
30265981	8	68	theme	3.2 nM	1333:1338	arg1	limit					1324:1328	detection limit	1314:1328	detection limit of 3.2 nM	1314:1338	Theophylline response was linearly within the range of 0.01-60.0 μM with detection limit of 3.2 nM.					
30265981	5	69	with	composite	776:784	arg1	morphology					799:808	netlike morphology	791:808	netlike morphology	791:808	The SA-MWCNTs composite with netlike morphology demonstrated high conductivity and electrocatalytic activity.					
30265981	4	70	theme	alginate/multiwalled	655:674	arg1	SA-MWCNTs					694:702	SA-MWCNTs	694:702	SA-MWCNTs	694:702	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	4	70	theme	alginate/multiwalled	655:674	arg1	nanotubes					683:691	sodium alginate/multiwalled carbon nanotubes	648:691	sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs)	648:703	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	1	71	theme	novel	256:260	arg1	film					294:297	a novel molecularly imprinted composite film	254:297	a novel molecularly imprinted composite film using the mathematical modeling	254:329	This paper reports on the synthesis of a novel molecularly imprinted composite film using the mathematical modeling.					
30265981	6	72	from	electrode	904:912	arg1	presence					941:948	the presence	937:948	the presence of theophylline	937:964	Cyclic voltammogram of modified electrode (MIP/SA-MWCNTs/GCE) in the presence of theophylline showed a sensitive anodic peak in 1170 mV in buffer solution of phosphate (pH 7.0).					
30265981	3	73	from	ratio	434:438	arg1	condition					474:482	optimum condition	466:482	optimum condition	466:482	The ratio of monomer to template in optimum condition was obtained to be 4.					
30265981	7	74	theme	modified	1221:1228	arg1	electrode					1230:1238	the modified electrode	1217:1238	the modified electrode	1217:1238	The investigation and optimization of the effective factors on the response and electrochemical behavior of target theophylline were accurately done on the surface of the modified electrode.					
30265981	10	75	from	samples	1511:1517	arg1	satisfactory					1453:1464	satisfactory	1453:1464	satisfactory	1453:1464	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	10	75	from	samples	1511:1517	arg1	function					1422:1429	The function	1418:1429	The function of this electrode	1418:1447	The function of this electrode was satisfactory in the determination of theophylline in real samples like theophylline tablet, theophylline oral solution and human plasma samples.					
30265981	4	76	theme	sodium	648:653	arg1	SA-MWCNTs					694:702	SA-MWCNTs	694:702	SA-MWCNTs	694:702	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30265981	4	76	theme	sodium	648:653	arg1	nanotubes					683:691	sodium alginate/multiwalled carbon nanotubes	648:691	sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs)	648:703	The modification of electrode was performed in the presence of theophylline through the electropolymerization of arginine on the composite of sodium alginate/multiwalled carbon nanotubes (SA-MWCNTs), which had been coated on glassy carbon electrode (GCE).					
30372963	0	0	theme	rice	71:74	arg1	powder					76:81	brown rice powder	65:81	brown rice powder	65:81	Effect of cooking methods on solubility and nutrition quality of brown rice powder.					
30372963	2	1	theme	different	320:328	arg1	methods					341:347	different processing methods	320:347	different processing methods	320:347	It showed that, except for flavonoids, different processing methods significantly influenced the indexes of the instant brown rice flour (p < 0.05).					
30372963	6	2	theme	rice	968:971	arg1	powder					973:978	a better quality instant brown rice powder	937:978	a better quality instant brown rice powder	937:978	Comparing with DGBRE, DBRE produced a better quality instant brown rice powder.					
30372963	0	3	theme	brown	65:69	arg1	powder					76:81	brown rice powder	65:81	brown rice powder	65:81	Effect of cooking methods on solubility and nutrition quality of brown rice powder.					
30372963	5	4	theme	agglomeration	866:878	arg1	time					895:898	dispersion time	884:898	dispersion time	884:898	The synergistic treatment of germination and exogenous enzymes significantly increased the digestibility of starch, but it deteriorated the fluidity, the rate of agglomeration and dispersion time.					
30372963	5	4	theme	agglomeration	866:878	arg1	fluidity					844:851	the fluidity	840:851	the fluidity	840:851	The synergistic treatment of germination and exogenous enzymes significantly increased the digestibility of starch, but it deteriorated the fluidity, the rate of agglomeration and dispersion time.					
30372963	5	4	theme	agglomeration	866:878	arg1	rate					858:861	the rate	854:861	the rate of agglomeration	854:878	The synergistic treatment of germination and exogenous enzymes significantly increased the digestibility of starch, but it deteriorated the fluidity, the rate of agglomeration and dispersion time.					
30372963	1	5	theme	aroma	233:237	arg1	constituent					239:249	aroma constituent	233:249	aroma constituent	233:249	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	0	6	from	Effect	0:5	arg1	solubility					29:38	solubility	29:38	solubility	29:38	Effect of cooking methods on solubility and nutrition quality of brown rice powder.					
30372963	0	6	from	Effect	0:5	arg1	quality					54:60	nutrition quality	44:60	nutrition quality	44:60	Effect of cooking methods on solubility and nutrition quality of brown rice powder.					
30372963	4	7	theme	Exogenous	562:570	arg1	treatment					579:587	Exogenous enzyme treatment	562:587	Exogenous enzyme treatment	562:587	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	0	8	theme	powder	76:81	arg1	solubility					29:38	solubility	29:38	solubility	29:38	Effect of cooking methods on solubility and nutrition quality of brown rice powder.					
30372963	0	8	theme	powder	76:81	arg1	quality					54:60	nutrition quality	44:60	nutrition quality	44:60	Effect of cooking methods on solubility and nutrition quality of brown rice powder.					
30372963	4	9	theme	protein	684:690	arg1	solubility					615:624	solubility	615:624	solubility	615:624	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	4	9	theme	protein	684:690	arg1	rate					676:679	the digestion rate	662:679	the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch	598:701	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	4	9	theme	protein	684:690	arg1	fluidity					602:609	fluidity	602:609	fluidity	602:609	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	6	10	theme	quality	946:952	arg1	powder					973:978	a better quality instant brown rice powder	937:978	a better quality instant brown rice powder	937:978	Comparing with DGBRE, DBRE produced a better quality instant brown rice powder.					
30372963	5	11	theme	germination	733:743	arg1	treatment					720:728	The synergistic treatment	704:728	The synergistic treatment of germination and exogenous enzymes	704:765	The synergistic treatment of germination and exogenous enzymes significantly increased the digestibility of starch, but it deteriorated the fluidity, the rate of agglomeration and dispersion time.					
30372963	4	12	theme	starch	696:701	arg1	solubility					615:624	solubility	615:624	solubility	615:624	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	4	12	theme	starch	696:701	arg1	rate					676:679	the digestion rate	662:679	the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch	598:701	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	4	12	theme	starch	696:701	arg1	fluidity					602:609	fluidity	602:609	fluidity	602:609	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	3	13	theme	powder	502:507	arg1	fluidity					464:471	fluidity	464:471	fluidity	464:471	Germination could deteriorate the fluidity and solubility of brown rice powder and reduce the digestibility of protein, and starch.					
30372963	3	13	theme	powder	502:507	arg1	solubility					477:486	solubility	477:486	solubility	477:486	Germination could deteriorate the fluidity and solubility of brown rice powder and reduce the digestibility of protein, and starch.					
30372963	4	14	theme	brown	633:637	arg1	powder					644:649	the brown rice powder	629:649	the brown rice powder	629:649	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	3	15	theme	rice	497:500	arg1	powder					502:507	brown rice powder	491:507	brown rice powder	491:507	Germination could deteriorate the fluidity and solubility of brown rice powder and reduce the digestibility of protein, and starch.					
30372963	6	16	theme	better	939:944	arg1	powder					973:978	a better quality instant brown rice powder	937:978	a better quality instant brown rice powder	937:978	Comparing with DGBRE, DBRE produced a better quality instant brown rice powder.					
30372963	3	17	theme	protein	541:547	arg1	digestibility					524:536	the digestibility	520:536	the digestibility of protein, and starch	520:559	Germination could deteriorate the fluidity and solubility of brown rice powder and reduce the digestibility of protein, and starch.					
30372963	4	18	theme	powder	644:649	arg1	solubility					615:624	solubility	615:624	solubility	615:624	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	4	18	theme	powder	644:649	arg1	rate					676:679	the digestion rate	662:679	the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch	598:701	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	4	18	theme	powder	644:649	arg1	fluidity					602:609	fluidity	602:609	fluidity	602:609	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	1	19	theme	instant	254:260	arg1	powder					273:278	instant brown rice powder	254:278	instant brown rice powder	254:278	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	4	20	theme	rice	639:642	arg1	powder					644:649	the brown rice powder	629:649	the brown rice powder	629:649	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	2	21	theme	flour	412:416	arg1	indexes					378:384	the indexes	374:384	the indexes of the instant brown rice flour (p < 0.05)	374:427	It showed that, except for flavonoids, different processing methods significantly influenced the indexes of the instant brown rice flour (p < 0.05).					
30372963	1	22	theme	brown	262:266	arg1	powder					273:278	instant brown rice powder	254:278	instant brown rice powder	254:278	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	4	23	dep	fluidity	602:609	arg1	the					598:600	the	598:600	the	598:600	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	2	24	theme	brown	401:405	arg1	p < 0.05					419:426	p < 0.05	419:426	p < 0.05	419:426	It showed that, except for flavonoids, different processing methods significantly influenced the indexes of the instant brown rice flour (p < 0.05).					
30372963	2	24	theme	brown	401:405	arg1	flour					412:416	the instant brown rice flour	389:416	the instant brown rice flour (p < 0.05)	389:427	It showed that, except for flavonoids, different processing methods significantly influenced the indexes of the instant brown rice flour (p < 0.05).					
30372963	2	25	theme	rice	407:410	arg1	p < 0.05					419:426	p < 0.05	419:426	p < 0.05	419:426	It showed that, except for flavonoids, different processing methods significantly influenced the indexes of the instant brown rice flour (p < 0.05).					
30372963	2	25	theme	rice	407:410	arg1	flour					412:416	the instant brown rice flour	389:416	the instant brown rice flour (p < 0.05)	389:427	It showed that, except for flavonoids, different processing methods significantly influenced the indexes of the instant brown rice flour (p < 0.05).					
30372963	1	26	theme	rice	268:271	arg1	powder					273:278	instant brown rice powder	254:278	instant brown rice powder	254:278	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	3	27	dep	fluidity	464:471	arg1	the					460:462	the	460:462	the	460:462	Germination could deteriorate the fluidity and solubility of brown rice powder and reduce the digestibility of protein, and starch.					
30372963	2	28	theme	instant	393:399	arg1	p < 0.05					419:426	p < 0.05	419:426	p < 0.05	419:426	It showed that, except for flavonoids, different processing methods significantly influenced the indexes of the instant brown rice flour (p < 0.05).					
30372963	2	28	theme	instant	393:399	arg1	flour					412:416	the instant brown rice flour	389:416	the instant brown rice flour (p < 0.05)	389:427	It showed that, except for flavonoids, different processing methods significantly influenced the indexes of the instant brown rice flour (p < 0.05).					
30372963	1	29	theme	powder	273:278	arg1	fluidity					164:171	fluidity	164:171	fluidity	164:171	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	1	29	theme	powder	273:278	arg1	digestibility					208:220	digestibility	208:220	digestibility	208:220	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	1	29	theme	powder	273:278	arg1	color					223:227	color	223:227	color	223:227	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	1	29	theme	powder	273:278	arg1	solubility					174:183	solubility	174:183	solubility	174:183	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	1	29	theme	powder	273:278	arg1	composition					195:205	nutrient composition	186:205	nutrient composition	186:205	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	1	29	theme	powder	273:278	arg1	constituent					239:249	aroma constituent	233:249	aroma constituent	233:249	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	1	30	dep	fluidity	164:171	arg1	the					160:162	the	160:162	the	160:162	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	1	31	theme	nutrient	186:193	arg1	composition					195:205	nutrient composition	186:205	nutrient composition	186:205	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	5	32	theme	exogenous	749:757	arg1	enzymes					759:765	exogenous enzymes	749:765	exogenous enzymes	749:765	The synergistic treatment of germination and exogenous enzymes significantly increased the digestibility of starch, but it deteriorated the fluidity, the rate of agglomeration and dispersion time.					
30372963	6	33	theme	brown	962:966	arg1	powder					973:978	a better quality instant brown rice powder	937:978	a better quality instant brown rice powder	937:978	Comparing with DGBRE, DBRE produced a better quality instant brown rice powder.					
30372963	0	34	theme	methods	18:24	arg1	Effect					0:5	Effect	0:5	Effect of cooking methods on solubility and nutrition quality of brown rice powder.	0:82	Effect of cooking methods on solubility and nutrition quality of brown rice powder.					
30372963	0	35	theme	nutrition	44:52	arg1	quality					54:60	nutrition quality	44:60	nutrition quality	44:60	Effect of cooking methods on solubility and nutrition quality of brown rice powder.					
30372963	3	36	theme	starch	554:559	arg1	digestibility					524:536	the digestibility	520:536	the digestibility of protein, and starch	520:559	Germination could deteriorate the fluidity and solubility of brown rice powder and reduce the digestibility of protein, and starch.					
30372963	1	37	theme	different	99:107	arg1	methods					120:126	different processing methods	99:126	different processing methods	99:126	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	5	38	theme	dispersion	884:893	arg1	time					895:898	dispersion time	884:898	dispersion time	884:898	The synergistic treatment of germination and exogenous enzymes significantly increased the digestibility of starch, but it deteriorated the fluidity, the rate of agglomeration and dispersion time.					
30372963	1	39	theme	processing	109:118	arg1	methods					120:126	different processing methods	99:126	different processing methods	99:126	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	6	40	theme	instant	954:960	arg1	powder					973:978	a better quality instant brown rice powder	937:978	a better quality instant brown rice powder	937:978	Comparing with DGBRE, DBRE produced a better quality instant brown rice powder.					
30372963	1	41	theme	methods	120:126	arg1	effects					88:94	The effects	84:94	The effects of different processing methods	84:126	The effects of different processing methods were investigated, including on the fluidity, solubility, nutrient composition, digestibility, color and aroma constituent of instant brown rice powder.					
30372963	4	42	theme	enzyme	572:577	arg1	treatment					579:587	Exogenous enzyme treatment	562:587	Exogenous enzyme treatment	562:587	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30372963	5	43	theme	synergistic	708:718	arg1	treatment					720:728	The synergistic treatment	704:728	The synergistic treatment of germination and exogenous enzymes	704:765	The synergistic treatment of germination and exogenous enzymes significantly increased the digestibility of starch, but it deteriorated the fluidity, the rate of agglomeration and dispersion time.					
30372963	5	44	theme	starch	812:817	arg1	digestibility					795:807	the digestibility	791:807	the digestibility of starch	791:817	The synergistic treatment of germination and exogenous enzymes significantly increased the digestibility of starch, but it deteriorated the fluidity, the rate of agglomeration and dispersion time.					
30372963	3	45	theme	brown	491:495	arg1	powder					502:507	brown rice powder	491:507	brown rice powder	491:507	Germination could deteriorate the fluidity and solubility of brown rice powder and reduce the digestibility of protein, and starch.					
30372963	5	46	theme	enzymes	759:765	arg1	treatment					720:728	The synergistic treatment	704:728	The synergistic treatment of germination and exogenous enzymes	704:765	The synergistic treatment of germination and exogenous enzymes significantly increased the digestibility of starch, but it deteriorated the fluidity, the rate of agglomeration and dispersion time.					
30372963	0	47	theme	cooking	10:16	arg1	methods					18:24	cooking methods	10:24	cooking methods	10:24	Effect of cooking methods on solubility and nutrition quality of brown rice powder.					
30372963	2	48	theme	processing	330:339	arg1	methods					341:347	different processing methods	320:347	different processing methods	320:347	It showed that, except for flavonoids, different processing methods significantly influenced the indexes of the instant brown rice flour (p < 0.05).					
30372963	4	49	theme	digestion	666:674	arg1	rate					676:679	the digestion rate	662:679	the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch	598:701	Exogenous enzyme treatment improved the fluidity and solubility of the brown rice powder as well as the digestion rate of protein and starch.					
30026100	2	0	theme	composite	366:374	arg1	films					376:380	PL/CS composite films	360:380	PL/CS composite films	360:380	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	1	1	theme	casting	226:232	arg1	method					234:239	casting method	226:239	casting method	226:239	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	8	2	theme	disease	1126:1132	arg1	incidence					1134:1142	the disease incidence	1122:1142	the disease incidence of citrus	1122:1152	Also the disease incidence of citrus was decreasing with the increase of PL content.					
30026100	1	3	theme	%	191:191	arg1	concentration					169:181	the PL concentration	162:181	the PL concentration of 0%, 1%, 3%, 5% and 7%	162:206	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	2	4	theme	PL/CS	360:364	arg1	films					376:380	PL/CS composite films	360:380	PL/CS composite films	360:380	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	7	5	theme	PL/CS	943:947	arg1	coatings					949:956	the PL/CS coatings	939:956	the PL/CS coatings	939:956	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	2	6	from	films	376:380	arg1	citrus					426:431	citrus	426:431	citrus	426:431	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	0	7	from	Development	0:10	arg1	citrus					82:87	citrus	82:87	citrus	82:87	Development of antibacterial ε-polylysine/chitosan hybrid films and the effect on citrus.					
30026100	4	8	theme	PL	603:604	arg1	addition					591:598	the addition	587:598	the addition of PL to chitosan	587:616	SEM images indicated that the film surface was smooth and the addition of PL to chitosan lead to more holes.					
30026100	9	9	dep	citrus	1283:1288	arg1	the					1272:1274	the	1272:1274	the	1272:1274	However, the weight loss did not show significant differences between the coated citrus and control during the whole storage period.					
30026100	9	10	theme	whole	1313:1317	arg1	period					1327:1332	the whole storage period	1309:1332	the whole storage period	1309:1332	However, the weight loss did not show significant differences between the coated citrus and control during the whole storage period.					
30026100	1	11	theme	%	195:195	arg1	concentration					169:181	the PL concentration	162:181	the PL concentration of 0%, 1%, 3%, 5% and 7%	162:206	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	7	12	theme	solids	1009:1014	arg1	decline					984:990	the decline	980:990	the decline of total soluble solids (TSS)	980:1020	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	7	12	theme	solids	1009:1014	arg1	content					1037:1043	ascorbic acid content	1023:1043	ascorbic acid content	1023:1043	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	7	13	theme	citrus	1082:1087	arg1	incidence					1069:1077	the disease incidence	1057:1077	the disease incidence of citrus	1057:1087	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	2	14	dep	infrared	264:271	arg1	SEM					311:313	SEM	311:313	SEM	311:313	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	2	14	dep	infrared	264:271	arg1	microscope					299:308	scanning electron microscope	281:308	scanning electron microscope (SEM)	281:314	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	2	14	dep	infrared	264:271	arg1	FTIR					274:277	FTIR	274:277	FTIR	274:277	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	9	15	theme	weight	1215:1220	arg1	loss					1222:1225	the weight loss	1211:1225	the weight loss	1211:1225	However, the weight loss did not show significant differences between the coated citrus and control during the whole storage period.					
30026100	2	16	from	citrus	426:431	arg1	properties					346:355	mechanical and antibacterial properties	317:355	mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus	317:431	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	2	17	from	effect	398:403	arg1	citrus					426:431	citrus	426:431	citrus	426:431	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	1	18	theme	composite	141:149	arg1	films					151:155	the ε-polylysine (PL)/chitosan (CS) composite films	105:155	the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7%	105:206	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	1	18	theme	composite	141:149	arg1	CS					137:138	CS	137:138	CS	137:138	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	2	19	theme	mechanical	317:326	arg1	properties					346:355	mechanical and antibacterial properties	317:355	mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus	317:431	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	2	20	from	properties	346:355	arg1	citrus					426:431	citrus	426:431	citrus	426:431	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	7	21	theme	ascorbic	1023:1030	arg1	acid					1032:1035	ascorbic acid	1023:1035	ascorbic acid content	1023:1043	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	5	22	theme	PL	749:750	arg1	content					752:758	PL content	749:758	PL content	749:758	The tensile strength was deceasing and the elongation at break of the films was elevating with the increase of PL content.					
30026100	7	23	theme	total	995:999	arg1	TSS					1017:1019	TSS	1017:1019	TSS	1017:1019	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	7	23	theme	total	995:999	arg1	solids					1009:1014	total soluble solids	995:1014	total soluble solids (TSS)	995:1020	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	6	24	theme	PL	896:897	arg1	content					899:905	PL content	896:905	PL content	896:905	The antibacterial activity against Staphylococcus aureus and Escherichia coli of PL/CS composite films was enhanced with increasing of PL content.					
30026100	7	25	theme	soluble	1001:1007	arg1	TSS					1017:1019	TSS	1017:1019	TSS	1017:1019	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	7	25	theme	soluble	1001:1007	arg1	solids					1009:1014	total soluble solids	995:1014	total soluble solids (TSS)	995:1020	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	5	26	theme	content	752:758	arg1	increase					737:744	the increase	733:744	the increase of PL content	733:758	The tensile strength was deceasing and the elongation at break of the films was elevating with the increase of PL content.					
30026100	0	27	theme	ε-polylysine/chitosan	29:49	arg1	films					58:62	antibacterial ε-polylysine/chitosan hybrid films	15:62	antibacterial ε-polylysine/chitosan hybrid films	15:62	Development of antibacterial ε-polylysine/chitosan hybrid films and the effect on citrus.					
30026100	2	28	theme	coatings	414:421	arg1	films					376:380	PL/CS composite films	360:380	PL/CS composite films	360:380	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	2	28	theme	coatings	414:421	arg1	effect					398:403	the effect	394:403	the effect of PL/CS coatings on citrus	394:431	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	8	29	theme	content	1193:1199	arg1	increase					1178:1185	the increase	1174:1185	the increase of PL content	1174:1199	Also the disease incidence of citrus was decreasing with the increase of PL content.					
30026100	9	30	theme	significant	1240:1250	arg1	differences					1252:1262	significant differences	1240:1262	significant differences between the coated citrus and control during the whole storage period	1240:1332	However, the weight loss did not show significant differences between the coated citrus and control during the whole storage period.					
30026100	0	31	theme	antibacterial	15:27	arg1	films					58:62	antibacterial ε-polylysine/chitosan hybrid films	15:62	antibacterial ε-polylysine/chitosan hybrid films	15:62	Development of antibacterial ε-polylysine/chitosan hybrid films and the effect on citrus.					
30026100	1	32	theme	%	199:199	arg1	concentration					169:181	the PL concentration	162:181	the PL concentration of 0%, 1%, 3%, 5% and 7%	162:206	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	2	33	theme	PL/CS	408:412	arg1	coatings					414:421	PL/CS coatings	408:421	PL/CS coatings	408:421	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	9	34	theme	storage	1319:1325	arg1	period					1327:1332	the whole storage period	1309:1332	the whole storage period	1309:1332	However, the weight loss did not show significant differences between the coated citrus and control during the whole storage period.					
30026100	1	35	theme	PL	166:167	arg1	concentration					169:181	the PL concentration	162:181	the PL concentration of 0%, 1%, 3%, 5% and 7%	162:206	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	0	36	theme	films	58:62	arg1	Development					0:10	Development	0:10	Development of antibacterial ε-polylysine/chitosan hybrid films	0:62	Development of antibacterial ε-polylysine/chitosan hybrid films and the effect on citrus.					
30026100	0	36	theme	films	58:62	arg1	effect					72:77	the effect	68:77	the effect on citrus	68:87	Development of antibacterial ε-polylysine/chitosan hybrid films and the effect on citrus.					
30026100	6	37	theme	antibacterial	765:777	arg1	activity					779:786	The antibacterial activity	761:786	The antibacterial activity against Staphylococcus aureus and Escherichia coli of PL/CS composite films	761:862	The antibacterial activity against Staphylococcus aureus and Escherichia coli of PL/CS composite films was enhanced with increasing of PL content.					
30026100	8	38	theme	PL	1190:1191	arg1	content					1193:1199	PL content	1190:1199	PL content	1190:1199	Also the disease incidence of citrus was decreasing with the increase of PL content.					
30026100	2	39	theme	electron	290:297	arg1	SEM					311:313	SEM	311:313	SEM	311:313	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	2	39	theme	electron	290:297	arg1	microscope					299:308	scanning electron microscope	281:308	scanning electron microscope (SEM)	281:314	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	0	40	theme	hybrid	51:56	arg1	films					58:62	antibacterial ε-polylysine/chitosan hybrid films	15:62	antibacterial ε-polylysine/chitosan hybrid films	15:62	Development of antibacterial ε-polylysine/chitosan hybrid films and the effect on citrus.					
30026100	2	41	theme	effect	398:403	arg1	properties					346:355	mechanical and antibacterial properties	317:355	mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus	317:431	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	3	42	theme	strong	489:494	arg1	bond					505:508	no strong hydrogen bond	486:508	no strong hydrogen bond between CS and PL	486:526	FTIR spectra suggested that there was no strong hydrogen bond between CS and PL.					
30026100	5	43	theme	tensile	642:648	arg1	strength					650:657	The tensile strength	638:657	The tensile strength	638:657	The tensile strength was deceasing and the elongation at break of the films was elevating with the increase of PL content.					
30026100	2	44	theme	scanning	281:288	arg1	SEM					311:313	SEM	311:313	SEM	311:313	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	2	44	theme	scanning	281:288	arg1	microscope					299:308	scanning electron microscope	281:308	scanning electron microscope (SEM)	281:314	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	2	45	theme	antibacterial	332:344	arg1	properties					346:355	mechanical and antibacterial properties	317:355	mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus	317:431	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	1	46	theme	%	206:206	arg1	concentration					169:181	the PL concentration	162:181	the PL concentration of 0%, 1%, 3%, 5% and 7%	162:206	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	1	47	with	films	151:155	arg1	concentration					169:181	the PL concentration	162:181	the PL concentration of 0%, 1%, 3%, 5% and 7%	162:206	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	0	48	from	effect	72:77	arg1	citrus					82:87	citrus	82:87	citrus	82:87	Development of antibacterial ε-polylysine/chitosan hybrid films and the effect on citrus.					
30026100	4	49	theme	film	559:562	arg1	smooth					576:581	smooth	576:581	smooth	576:581	SEM images indicated that the film surface was smooth and the addition of PL to chitosan lead to more holes.					
30026100	4	49	theme	film	559:562	arg1	surface					564:570	the film surface	555:570	the film surface	555:570	SEM images indicated that the film surface was smooth and the addition of PL to chitosan lead to more holes.					
30026100	3	50	theme	hydrogen	496:503	arg1	bond					505:508	no strong hydrogen bond	486:508	no strong hydrogen bond between CS and PL	486:526	FTIR spectra suggested that there was no strong hydrogen bond between CS and PL.					
30026100	3	51	theme	FTIR	448:451	arg1	spectra					453:459	FTIR spectra	448:459	FTIR spectra	448:459	FTIR spectra suggested that there was no strong hydrogen bond between CS and PL.					
30026100	7	52	theme	disease	1061:1067	arg1	incidence					1069:1077	the disease incidence	1057:1077	the disease incidence of citrus	1057:1087	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	6	53	theme	composite	848:856	arg1	films					858:862	PL/CS composite films	842:862	PL/CS composite films	842:862	The antibacterial activity against Staphylococcus aureus and Escherichia coli of PL/CS composite films was enhanced with increasing of PL content.					
30026100	6	54	theme	films	858:862	arg1	activity					779:786	The antibacterial activity	761:786	The antibacterial activity against Staphylococcus aureus and Escherichia coli of PL/CS composite films	761:862	The antibacterial activity against Staphylococcus aureus and Escherichia coli of PL/CS composite films was enhanced with increasing of PL content.					
30026100	8	55	theme	citrus	1147:1152	arg1	incidence					1134:1142	the disease incidence	1122:1142	the disease incidence of citrus	1122:1152	Also the disease incidence of citrus was decreasing with the increase of PL content.					
30026100	5	56	from	break	695:699	arg1	elongation					681:690	the elongation	677:690	the elongation at break of the films	677:712	The tensile strength was deceasing and the elongation at break of the films was elevating with the increase of PL content.					
30026100	4	57	theme	SEM	529:531	arg1	images					533:538	SEM images	529:538	SEM images	529:538	SEM images indicated that the film surface was smooth and the addition of PL to chitosan lead to more holes.					
30026100	1	58	theme	%	187:187	arg1	concentration					169:181	the PL concentration	162:181	the PL concentration of 0%, 1%, 3%, 5% and 7%	162:206	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	7	59	theme	acid	1032:1035	arg1	decline					984:990	the decline	980:990	the decline of total soluble solids (TSS)	980:1020	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	7	59	theme	acid	1032:1035	arg1	content					1037:1043	ascorbic acid content	1023:1043	ascorbic acid content	1023:1043	Furthermore, it was found that the PL/CS coatings inhibited efficiently the decline of total soluble solids (TSS), ascorbic acid content and reduced the disease incidence of citrus, compared with the control.					
30026100	5	60	theme	films	708:712	arg1	elongation					681:690	the elongation	677:690	the elongation at break of the films	677:712	The tensile strength was deceasing and the elongation at break of the films was elevating with the increase of PL content.					
30026100	6	61	theme	PL/CS	842:846	arg1	films					858:862	PL/CS composite films	842:862	PL/CS composite films	842:862	The antibacterial activity against Staphylococcus aureus and Escherichia coli of PL/CS composite films was enhanced with increasing of PL content.					
30026100	2	62	theme	films	376:380	arg1	properties					346:355	mechanical and antibacterial properties	317:355	mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus	317:431	The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of PL/CS coatings on citrus were observed.					
30026100	4	63	theme	more	626:629	arg1	holes					631:635	more holes	626:635	more holes	626:635	SEM images indicated that the film surface was smooth and the addition of PL to chitosan lead to more holes.					
30026100	1	64	theme	/chitosan	126:134	arg1	films					151:155	the ε-polylysine (PL)/chitosan (CS) composite films	105:155	the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7%	105:206	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
30026100	1	64	theme	/chitosan	126:134	arg1	CS					137:138	CS	137:138	CS	137:138	In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method.					
31426033	6	0	theme	structural	838:847	arg1	integrity					849:857	Long-term structural integrity	828:857	Long-term structural integrity of the printed scaffolds	828:882	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	4	1	theme	properties	558:567	arg1	changes					538:544	the changes	534:544	the changes of physical properties affected by the alginate concentration	534:606	The aim was to investigate the changes of physical properties affected by the alginate concentration and the influences on cell behavior.					
31426033	4	1	theme	properties	558:567	arg1	influences					616:625	the influences	612:625	the influences on cell behavior	612:642	The aim was to investigate the changes of physical properties affected by the alginate concentration and the influences on cell behavior.					
31426033	2	2	theme	scaffold	285:292	arg1	properties					294:303	physical scaffold properties	276:303	physical scaffold properties	276:303	However, the influence of physical scaffold properties on cell behavior is still unclear.					
31426033	4	3	theme	cell	630:633	arg1	behavior					635:642	cell behavior	630:642	cell behavior	630:642	The aim was to investigate the changes of physical properties affected by the alginate concentration and the influences on cell behavior.					
31426033	10	4	theme	cell	1485:1488	arg1	viability					1490:1498	higher cell viability	1478:1498	higher cell viability (84% ± 0.7% versus 68% ± 1.3%)	1478:1529	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	4	theme	cell	1485:1488	arg1	%					1503:1503	84% ± 0.7% versus 68% ± 1.3%	1501:1528	%	1503:1503	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	4	theme	cell	1485:1488	arg1	%					1521:1521	68%	1519:1521	68%	1519:1521	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	5	dep	%	1503:1503	arg1	%					1510:1510	 ± 0.7%	1504:1510	 ± 0.7%	1504:1510	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	5	dep	%	1503:1503	arg1	%					1528:1528	 ± 1.3%	1522:1528	 ± 1.3%	1522:1528	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	7	6	theme	higher	1088:1093	arg1	concentration					1104:1116	higher alginate concentration	1088:1116	higher alginate concentration	1088:1116	Scaffold structure, swelling behavior, and compressive moduli decreased with culturing time and higher alginate concentration lead to more stable physical scaffold properties.					
31426033	14	7	theme	alginate	2041:2048	arg1	scaffolds					2064:2072	lower alginate concentration scaffolds	2035:2072	lower alginate concentration scaffolds	2035:2072	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	7	8	theme	Scaffold	992:999	arg1	structure					1001:1009	Scaffold structure	992:1009	Scaffold structure	992:1009	Scaffold structure, swelling behavior, and compressive moduli decreased with culturing time and higher alginate concentration lead to more stable physical scaffold properties.					
31426033	4	9	from	influences	616:625	arg1	behavior					635:642	cell behavior	630:642	cell behavior	630:642	The aim was to investigate the changes of physical properties affected by the alginate concentration and the influences on cell behavior.					
31426033	9	10	theme	cell	1386:1389	arg1	viability					1391:1399	cell viability	1386:1399	cell viability	1386:1399	Bioprinting did not affect cell viability, but alginate concentration had a significant influence on cell viability and cell morphology.					
31426033	12	11	theme	design	1815:1820	arg1	space					1822:1826	the design space	1811:1826	the design space for alginate-based bioinks for 3D bioprinting	1811:1872	In conclusion, this study broadens the design space for alginate-based bioinks for 3D bioprinting.					
31426033	8	12	theme	mesenchymal	1185:1195	arg1	hMSCs					1208:1212	hMSCs	1208:1212	hMSCs	1208:1212	Meanwhile, human mesenchymal stem cell (hMSCs) laden non-printed and bioprinted composite scaffolds were fabricated.					
31426033	8	12	theme	mesenchymal	1185:1195	arg1	cell					1202:1205	mesenchymal stem cell	1185:1205	human mesenchymal stem cell (hMSCs) laden non-printed	1179:1231	Meanwhile, human mesenchymal stem cell (hMSCs) laden non-printed and bioprinted composite scaffolds were fabricated.					
31426033	5	13	theme	different	671:679	arg1	scaffolds					704:712	the different alginate concentration scaffolds	667:712	the different alginate concentration scaffolds	667:712	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	5	14	theme	%	799:799	arg1	alg					800:802	0.8%alg	796:802	0.8%alg	796:802	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	5	14	theme	%	799:799	arg1	kPa					791:793	1.5 kPa	787:793	1.5 kPa (0.8%alg)	787:803	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	8	15	theme	cell	1202:1205	arg1	non-printed					1221:1231	human mesenchymal stem cell (hMSCs) laden non-printed	1179:1231	human mesenchymal stem cell (hMSCs) laden non-printed	1179:1231	Meanwhile, human mesenchymal stem cell (hMSCs) laden non-printed and bioprinted composite scaffolds were fabricated.					
31426033	11	16	theme	3D	1677:1678	arg1	network					1704:1710	a 3D interconnected cellular network	1675:1710	a 3D interconnected cellular network	1675:1710	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	5	17	theme	concentration	690:702	arg1	scaffolds					704:712	the different alginate concentration scaffolds	667:712	the different alginate concentration scaffolds	667:712	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	1	18	theme	tissue	218:223	arg1	repair					225:230	bone tissue repair	213:230	bone tissue repair	213:230	Three-dimensional (3D) cell-laden scaffolds are becoming more prevalent in bone tissue repair and regeneration.					
31426033	6	19	theme	printed	866:872	arg1	scaffolds					874:882	the printed scaffolds	862:882	the printed scaffolds	862:882	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	14	20	theme	spreading	1998:2006	arg1	morphology					2008:2017	cell spreading morphology	1993:2017	cell spreading morphology	1993:2017	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	13	21	theme	mechanical	1944:1953	arg1	properties					1955:1964	mechanical properties	1944:1964	mechanical properties	1944:1964	Higher alginate concentration preserved better scaffold fidelity and mechanical properties.					
31426033	1	22	from	prevalent	200:208	arg1	regeneration					236:247	regeneration	236:247	regeneration	236:247	Three-dimensional (3D) cell-laden scaffolds are becoming more prevalent in bone tissue repair and regeneration.					
31426033	1	22	from	prevalent	200:208	arg1	repair					225:230	bone tissue repair	213:230	bone tissue repair	213:230	Three-dimensional (3D) cell-laden scaffolds are becoming more prevalent in bone tissue repair and regeneration.					
31426033	11	23	from	image	1614:1618	arg1	scaffolds					1635:1643	the 0.8%alg scaffolds	1623:1643	the 0.8%alg scaffolds	1623:1643	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	8	24	theme	laden	1215:1219	arg1	non-printed					1221:1231	human mesenchymal stem cell (hMSCs) laden non-printed	1179:1231	human mesenchymal stem cell (hMSCs) laden non-printed	1179:1231	Meanwhile, human mesenchymal stem cell (hMSCs) laden non-printed and bioprinted composite scaffolds were fabricated.					
31426033	10	25	dep	showed	1471:1476	arg1	compared					1531:1538	compared	1531:1538	showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14	1471:1601	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	1	26	theme	Three-dimensional	138:154	arg1	scaffolds					172:180	Three-dimensional (3D) cell-laden scaffolds	138:180	Three-dimensional (3D) cell-laden scaffolds	138:180	Three-dimensional (3D) cell-laden scaffolds are becoming more prevalent in bone tissue repair and regeneration.					
31426033	11	27	theme	Live	1604:1607	arg1	image					1614:1618	Live cell image	1604:1618	Live cell image in the 0.8%alg scaffolds	1604:1643	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	7	28	theme	scaffold	1147:1154	arg1	properties					1156:1165	more stable physical scaffold properties	1126:1165	more stable physical scaffold properties	1126:1165	Scaffold structure, swelling behavior, and compressive moduli decreased with culturing time and higher alginate concentration lead to more stable physical scaffold properties.					
31426033	13	29	theme	better	1915:1920	arg1	fidelity					1931:1938	better scaffold fidelity	1915:1938	better scaffold fidelity	1915:1938	Higher alginate concentration preserved better scaffold fidelity and mechanical properties.					
31426033	5	30	theme	%	821:821	arg1	alg					822:824	2.3%alg	818:824	2.3%alg	818:824	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	5	30	theme	%	821:821	arg1	kPa					813:815	14.2 kPa	808:815	14.2 kPa (2.3%alg)	808:825	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	0	31	from	changes	19:25	arg1	scaffolds					85:93	3D bioprinted cell-laden porous scaffolds	53:93	3D bioprinted cell-laden porous scaffolds	53:93	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	14	32	theme	cell	1993:1996	arg1	morphology					2008:2017	cell spreading morphology	1993:2017	cell spreading morphology	1993:2017	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	8	33	theme	composite	1248:1256	arg1	scaffolds					1258:1266	bioprinted composite scaffolds	1237:1266	bioprinted composite scaffolds	1237:1266	Meanwhile, human mesenchymal stem cell (hMSCs) laden non-printed and bioprinted composite scaffolds were fabricated.					
31426033	11	34	theme	%	1630:1630	arg1	scaffolds					1635:1643	the 0.8%alg scaffolds	1623:1643	the 0.8%alg scaffolds	1623:1643	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	0	35	theme	porous	78:83	arg1	scaffolds					85:93	3D bioprinted cell-laden porous scaffolds	53:93	3D bioprinted cell-laden porous scaffolds	53:93	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	1	36	theme	cell-laden	161:170	arg1	scaffolds					172:180	Three-dimensional (3D) cell-laden scaffolds	138:180	Three-dimensional (3D) cell-laden scaffolds	138:180	Three-dimensional (3D) cell-laden scaffolds are becoming more prevalent in bone tissue repair and regeneration.					
31426033	6	37	theme	culture	919:925	arg1	media					927:931	cell culture media	914:931	cell culture media	914:931	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	13	38	theme	Higher	1875:1880	arg1	concentration					1891:1903	Higher alginate concentration	1875:1903	Higher alginate concentration	1875:1903	Higher alginate concentration preserved better scaffold fidelity and mechanical properties.					
31426033	14	39	theme	Better	1967:1972	arg1	viability					1979:1987	Better cell viability	1967:1987	Better cell viability	1967:1987	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	10	40	theme	higher	1543:1548	arg1	alg					1588:1590	2.3%alg	1584:1590	2.3%alg	1584:1590	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	40	theme	higher	1543:1548	arg1	scaffolds					1573:1581	higher alginate concentration scaffolds	1543:1581	higher alginate concentration scaffolds (2.3%alg)	1543:1591	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	4	41	from	changes	538:544	arg1	behavior					635:642	cell behavior	630:642	cell behavior	630:642	The aim was to investigate the changes of physical properties affected by the alginate concentration and the influences on cell behavior.					
31426033	3	42	theme	different	374:382	arg1	scaffolds					463:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds	369:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique	369:504	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	3	42	theme	different	374:382	arg1	alg					430:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	11	43	theme	cellular	1695:1702	arg1	network					1704:1710	a 3D interconnected cellular network	1675:1710	a 3D interconnected cellular network	1675:1710	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	0	44	theme	cell-laden	67:76	arg1	scaffolds					85:93	3D bioprinted cell-laden porous scaffolds	53:93	3D bioprinted cell-laden porous scaffolds	53:93	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	0	45	theme	Alginate	0:7	arg1	changes					19:25	Alginate dependent changes	0:25	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds	0:93	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	10	46	theme	concentration	1437:1449	arg1	alg					1466:1468	0.8%alg	1462:1468	0.8%alg	1462:1468	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	46	theme	concentration	1437:1449	arg1	scaffolds					1451:1459	Lower alginate concentration scaffolds	1422:1459	Lower alginate concentration scaffolds (0.8%alg)	1422:1469	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	14	47	theme	tissue	2128:2133	arg1	engineering					2135:2145	bone tissue engineering	2123:2145	bone tissue engineering	2123:2145	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	2	48	from	influence	263:271	arg1	behavior					313:320	cell behavior	308:320	cell behavior	308:320	However, the influence of physical scaffold properties on cell behavior is still unclear.					
31426033	6	49	theme	phosphate	959:967	arg1	PBS					986:988	PBS	986:988	PBS	986:988	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	6	49	theme	phosphate	959:967	arg1	saline					978:983	phosphate buffered saline	959:983	phosphate buffered saline (PBS)	959:989	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	10	50	theme	Lower	1422:1426	arg1	alg					1466:1468	0.8%alg	1462:1468	0.8%alg	1462:1468	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	50	theme	Lower	1422:1426	arg1	scaffolds					1451:1459	Lower alginate concentration scaffolds	1422:1459	Lower alginate concentration scaffolds (0.8%alg)	1422:1469	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	51	theme	concentration	1559:1571	arg1	alg					1588:1590	2.3%alg	1584:1590	2.3%alg	1584:1590	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	51	theme	concentration	1559:1571	arg1	scaffolds					1573:1581	higher alginate concentration scaffolds	1543:1581	higher alginate concentration scaffolds (2.3%alg)	1543:1591	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	0	52	theme	physical	30:37	arg1	properties					39:48	physical properties	30:48	physical properties	30:48	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	5	53	theme	compressive	755:765	arg1	moduli					767:772	compressive moduli	755:772	compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg)	755:825	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	3	54	theme	concentration	393:405	arg1	scaffolds					463:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds	369:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique	369:504	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	3	54	theme	concentration	393:405	arg1	alg					430:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	10	55	theme	%	1465:1465	arg1	alg					1466:1468	0.8%alg	1462:1468	0.8%alg	1462:1468	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	55	theme	%	1465:1465	arg1	scaffolds					1451:1459	Lower alginate concentration scaffolds	1422:1459	Lower alginate concentration scaffolds (0.8%alg)	1422:1469	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	0	56	theme	bioprinted	56:65	arg1	scaffolds					85:93	3D bioprinted cell-laden porous scaffolds	53:93	3D bioprinted cell-laden porous scaffolds	53:93	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	3	57	theme	porous	456:461	arg1	scaffolds					463:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds	369:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique	369:504	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	3	57	theme	porous	456:461	arg1	alg					430:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	10	58	theme	%	1587:1587	arg1	alg					1588:1590	2.3%alg	1584:1590	2.3%alg	1584:1590	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	58	theme	%	1587:1587	arg1	scaffolds					1573:1581	higher alginate concentration scaffolds	1543:1581	higher alginate concentration scaffolds (2.3%alg)	1543:1591	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	9	59	theme	cell	1312:1315	arg1	viability					1317:1325	cell viability	1312:1325	cell viability	1312:1325	Bioprinting did not affect cell viability, but alginate concentration had a significant influence on cell viability and cell morphology.					
31426033	10	60	theme	higher	1478:1483	arg1	viability					1490:1498	higher cell viability	1478:1498	higher cell viability (84% ± 0.7% versus 68% ± 1.3%)	1478:1529	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	60	theme	higher	1478:1483	arg1	%					1503:1503	84% ± 0.7% versus 68% ± 1.3%	1501:1528	%	1503:1503	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	60	theme	higher	1478:1483	arg1	%					1521:1521	68%	1519:1521	68%	1519:1521	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	3	61	theme	%	429:429	arg1	scaffolds					463:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds	369:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique	369:504	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	3	61	theme	%	429:429	arg1	alg					430:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	3	62	theme	3D	481:482	arg1	technique					496:504	a 3D bioprinting technique	479:504	a 3D bioprinting technique	479:504	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	0	63	theme	3D	53:54	arg1	scaffolds					85:93	3D bioprinted cell-laden porous scaffolds	53:93	3D bioprinted cell-laden porous scaffolds	53:93	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	9	64	theme	alginate	1332:1339	arg1	concentration					1341:1353	alginate concentration	1332:1353	alginate concentration	1332:1353	Bioprinting did not affect cell viability, but alginate concentration had a significant influence on cell viability and cell morphology.					
31426033	6	65	theme	Long-term	828:836	arg1	integrity					849:857	Long-term structural integrity	828:857	Long-term structural integrity of the printed scaffolds	828:882	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	7	66	theme	swelling	1012:1019	arg1	behavior					1021:1028	swelling behavior	1012:1028	swelling behavior	1012:1028	Scaffold structure, swelling behavior, and compressive moduli decreased with culturing time and higher alginate concentration lead to more stable physical scaffold properties.					
31426033	2	67	theme	properties	294:303	arg1	unclear					331:337	unclear	331:337	unclear	331:337	However, the influence of physical scaffold properties on cell behavior is still unclear.					
31426033	2	67	theme	properties	294:303	arg1	influence					263:271	the influence	259:271	the influence of physical scaffold properties on cell behavior	259:320	However, the influence of physical scaffold properties on cell behavior is still unclear.					
31426033	11	68	theme	network	1704:1710	arg1	formation					1662:1670	the formation	1658:1670	the formation of a 3D interconnected cellular network	1658:1710	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	4	69	theme	physical	549:556	arg1	properties					558:567	physical properties	549:567	physical properties affected by the alginate concentration	549:606	The aim was to investigate the changes of physical properties affected by the alginate concentration and the influences on cell behavior.					
31426033	2	70	theme	physical	276:283	arg1	properties					294:303	physical scaffold properties	276:303	physical scaffold properties	276:303	However, the influence of physical scaffold properties on cell behavior is still unclear.					
31426033	9	71	theme	significant	1361:1371	arg1	influence					1373:1381	a significant influence	1359:1381	a significant influence	1359:1381	Bioprinting did not affect cell viability, but alginate concentration had a significant influence on cell viability and cell morphology.					
31426033	12	72	theme	alginate-based	1832:1845	arg1	bioinks					1847:1853	alginate-based bioinks	1832:1853	alginate-based bioinks for 3D bioprinting	1832:1872	In conclusion, this study broadens the design space for alginate-based bioinks for 3D bioprinting.					
31426033	14	73	theme	concentration	2050:2062	arg1	scaffolds					2064:2072	lower alginate concentration scaffolds	2035:2072	lower alginate concentration scaffolds	2035:2072	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	7	74	theme	stable	1131:1136	arg1	properties					1156:1165	more stable physical scaffold properties	1126:1165	more stable physical scaffold properties	1126:1165	Scaffold structure, swelling behavior, and compressive moduli decreased with culturing time and higher alginate concentration lead to more stable physical scaffold properties.					
31426033	9	75	contain	had	1355:1357	arg1	concentration					1341:1353	alginate concentration	1332:1353	alginate concentration	1332:1353	Bioprinting did not affect cell viability, but alginate concentration had a significant influence on cell viability and cell morphology.					
31426033	9	75	contain	had	1355:1357	arg2	influence					1373:1381	a significant influence	1359:1381	a significant influence	1359:1381	Bioprinting did not affect cell viability, but alginate concentration had a significant influence on cell viability and cell morphology.					
31426033	14	76	theme	lower	2035:2039	arg1	scaffolds					2064:2072	lower alginate concentration scaffolds	2035:2072	lower alginate concentration scaffolds	2035:2072	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	2	77	theme	cell	308:311	arg1	behavior					313:320	cell behavior	308:320	cell behavior	308:320	However, the influence of physical scaffold properties on cell behavior is still unclear.					
31426033	8	78	theme	human	1179:1183	arg1	non-printed					1221:1231	human mesenchymal stem cell (hMSCs) laden non-printed	1179:1231	human mesenchymal stem cell (hMSCs) laden non-printed	1179:1231	Meanwhile, human mesenchymal stem cell (hMSCs) laden non-printed and bioprinted composite scaffolds were fabricated.					
31426033	5	79	theme	alginate	681:688	arg1	scaffolds					704:712	the different alginate concentration scaffolds	667:712	the different alginate concentration scaffolds	667:712	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	1	80	theme	bone	213:216	arg1	repair					225:230	bone tissue repair	213:230	bone tissue repair	213:230	Three-dimensional (3D) cell-laden scaffolds are becoming more prevalent in bone tissue repair and regeneration.					
31426033	6	81	theme	scaffolds	874:882	arg1	integrity					849:857	Long-term structural integrity	828:857	Long-term structural integrity of the printed scaffolds	828:882	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	8	82	theme	stem	1197:1200	arg1	hMSCs					1208:1212	hMSCs	1208:1212	hMSCs	1208:1212	Meanwhile, human mesenchymal stem cell (hMSCs) laden non-printed and bioprinted composite scaffolds were fabricated.					
31426033	8	82	theme	stem	1197:1200	arg1	cell					1202:1205	mesenchymal stem cell	1185:1205	human mesenchymal stem cell (hMSCs) laden non-printed	1179:1231	Meanwhile, human mesenchymal stem cell (hMSCs) laden non-printed and bioprinted composite scaffolds were fabricated.					
31426033	11	83	theme	interconnected	1680:1693	arg1	network					1704:1710	a 3D interconnected cellular network	1675:1710	a 3D interconnected cellular network	1675:1710	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	1	84	from	regeneration	236:247	arg1	prevalent					200:208	prevalent	200:208	prevalent	200:208	Three-dimensional (3D) cell-laden scaffolds are becoming more prevalent in bone tissue repair and regeneration.					
31426033	1	85	from	repair	225:230	arg1	prevalent					200:208	prevalent	200:208	prevalent	200:208	Three-dimensional (3D) cell-laden scaffolds are becoming more prevalent in bone tissue repair and regeneration.					
31426033	3	86	theme	composite	435:443	arg1	scaffolds					463:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds	369:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique	369:504	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	3	86	theme	composite	435:443	arg1	alg					430:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	7	87	theme	physical	1138:1145	arg1	properties					1156:1165	more stable physical scaffold properties	1126:1165	more stable physical scaffold properties	1126:1165	Scaffold structure, swelling behavior, and compressive moduli decreased with culturing time and higher alginate concentration lead to more stable physical scaffold properties.					
31426033	14	88	from	applications	2107:2118	arg1	engineering					2135:2145	bone tissue engineering	2123:2145	bone tissue engineering	2123:2145	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	11	89	theme	cell	1609:1612	arg1	image					1614:1618	Live cell image	1604:1618	Live cell image in the 0.8%alg scaffolds	1604:1643	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	13	90	theme	scaffold	1922:1929	arg1	fidelity					1931:1938	better scaffold fidelity	1915:1938	better scaffold fidelity	1915:1938	Higher alginate concentration preserved better scaffold fidelity and mechanical properties.					
31426033	0	91	theme	cell	121:124	arg1	morphology					126:135	cell morphology	121:135	cell morphology	121:135	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	11	92	from	cells	1719:1723	arg1	scaffolds					1748:1756	the 1.8 and 2.3%alg scaffolds	1728:1756	the 1.8 and 2.3%alg scaffolds	1728:1756	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	8	93	theme	bioprinted	1237:1246	arg1	scaffolds					1258:1266	bioprinted composite scaffolds	1237:1266	bioprinted composite scaffolds	1237:1266	Meanwhile, human mesenchymal stem cell (hMSCs) laden non-printed and bioprinted composite scaffolds were fabricated.					
31426033	11	94	theme	alg	1631:1633	arg1	scaffolds					1635:1643	the 0.8%alg scaffolds	1623:1643	the 0.8%alg scaffolds	1623:1643	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	0	95	theme	cell	102:105	arg1	viability					107:115	cell viability	102:115	cell viability	102:115	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	6	96	theme	cell	914:917	arg1	media					927:931	cell culture media	914:931	cell culture media	914:931	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	14	97	theme	cell	1974:1977	arg1	viability					1979:1987	Better cell viability	1967:1987	Better cell viability	1967:1987	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	1	98	dep	Three-dimensional	138:154	arg1	3D					157:158	3D	157:158	3D	157:158	Three-dimensional (3D) cell-laden scaffolds are becoming more prevalent in bone tissue repair and regeneration.					
31426033	11	99	theme	0.8	1627:1629	arg1	%					1630:1630	%	1630:1630	%	1630:1630	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	5	100	theme	uniform	718:724	arg1	macropores					726:735	uniform macropores	718:735	uniform macropores (500-600 μm)	718:748	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	5	100	theme	uniform	718:724	arg1	μm					746:747	500-600 μm	738:747	500-600 μm	738:747	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	13	101	theme	alginate	1882:1889	arg1	concentration					1891:1903	Higher alginate concentration	1875:1903	Higher alginate concentration	1875:1903	Higher alginate concentration preserved better scaffold fidelity and mechanical properties.					
31426033	5	102	contain	had	714:716	arg1	scaffolds					704:712	the different alginate concentration scaffolds	667:712	the different alginate concentration scaffolds	667:712	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	5	102	contain	had	714:716	arg2	μm					746:747	500-600 μm	738:747	500-600 μm	738:747	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	5	102	contain	had	714:716	arg2	macropores					726:735	uniform macropores	718:735	uniform macropores (500-600 μm)	718:748	The study showed that the different alginate concentration scaffolds had uniform macropores (500-600 μm) with compressive moduli ranging from 1.5 kPa (0.8%alg) to 14.2 kPa (2.3%alg).					
31426033	7	103	theme	alginate	1095:1102	arg1	concentration					1104:1116	higher alginate concentration	1088:1116	higher alginate concentration	1088:1116	Scaffold structure, swelling behavior, and compressive moduli decreased with culturing time and higher alginate concentration lead to more stable physical scaffold properties.					
31426033	0	104	theme	dependent	9:17	arg1	changes					19:25	Alginate dependent changes	0:25	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds	0:93	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	3	105	theme	alginate	384:391	arg1	scaffolds					463:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds	369:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique	369:504	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	3	105	theme	alginate	384:391	arg1	alg					430:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	7	106	theme	culturing	1069:1077	arg1	time					1079:1082	culturing time	1069:1082	culturing time	1069:1082	Scaffold structure, swelling behavior, and compressive moduli decreased with culturing time and higher alginate concentration lead to more stable physical scaffold properties.					
31426033	6	107	theme	buffered	969:976	arg1	PBS					986:988	PBS	986:988	PBS	986:988	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	6	107	theme	buffered	969:976	arg1	saline					978:983	phosphate buffered saline	959:983	phosphate buffered saline (PBS)	959:989	Long-term structural integrity of the printed scaffolds was achieved when cultured in cell culture media, but not when cultured in phosphate buffered saline (PBS).					
31426033	10	108	theme	alginate	1428:1435	arg1	alg					1466:1468	0.8%alg	1462:1468	0.8%alg	1462:1468	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	108	theme	alginate	1428:1435	arg1	scaffolds					1451:1459	Lower alginate concentration scaffolds	1422:1459	Lower alginate concentration scaffolds (0.8%alg)	1422:1469	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	14	109	theme	bone	2123:2126	arg1	engineering					2135:2145	bone tissue engineering	2123:2145	bone tissue engineering	2123:2145	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	0	110	theme	properties	39:48	arg1	changes					19:25	Alginate dependent changes	0:25	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds	0:93	Alginate dependent changes of physical properties in 3D bioprinted cell-laden porous scaffolds affect cell viability and cell morphology.					
31426033	9	111	theme	cell	1405:1408	arg1	morphology					1410:1419	cell morphology	1405:1419	cell morphology	1405:1419	Bioprinting did not affect cell viability, but alginate concentration had a significant influence on cell viability and cell morphology.					
31426033	10	112	theme	alginate	1550:1557	arg1	alg					1588:1590	2.3%alg	1584:1590	2.3%alg	1584:1590	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	10	112	theme	alginate	1550:1557	arg1	scaffolds					1573:1581	higher alginate concentration scaffolds	1543:1581	higher alginate concentration scaffolds (2.3%alg)	1543:1591	Lower alginate concentration scaffolds (0.8%alg) showed higher cell viability (84% ± 0.7% versus 68% ± 1.3%) compared to higher alginate concentration scaffolds (2.3%alg) at day 14.					
31426033	7	113	theme	compressive	1035:1045	arg1	moduli					1047:1052	compressive moduli	1035:1052	compressive moduli	1035:1052	Scaffold structure, swelling behavior, and compressive moduli decreased with culturing time and higher alginate concentration lead to more stable physical scaffold properties.					
31426033	11	114	dep	%	1743:1743	arg1	2.3					1740:1742	2.3	1740:1742	2.3	1740:1742	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	11	114	dep	%	1743:1743	arg1	1.8					1732:1734	1.8	1732:1734	1.8	1732:1734	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	11	115	dep	scaffolds	1748:1756	arg1	%					1743:1743	%	1743:1743	%	1743:1743	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	3	116	theme	cell-laden	445:454	arg1	scaffolds					463:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds	369:471	four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique	369:504	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	3	116	theme	cell-laden	445:454	arg1	alg					430:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	0.8, 1.3, 1.8 and 2.3%alg	408:432	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31426033	12	117	theme	3D	1859:1860	arg1	bioprinting					1862:1872	3D bioprinting	1859:1872	3D bioprinting	1859:1872	In conclusion, this study broadens the design space for alginate-based bioinks for 3D bioprinting.					
31426033	11	118	theme	alg	1744:1746	arg1	scaffolds					1748:1756	the 1.8 and 2.3%alg scaffolds	1728:1756	the 1.8 and 2.3%alg scaffolds	1728:1756	Live cell image in the 0.8%alg scaffolds demonstrated the formation of a 3D interconnected cellular network, while cells in the 1.8 and 2.3%alg scaffolds formed spheroids.					
31426033	4	119	theme	alginate	585:592	arg1	concentration					594:606	the alginate concentration	581:606	the alginate concentration	581:606	The aim was to investigate the changes of physical properties affected by the alginate concentration and the influences on cell behavior.					
31426033	14	120	theme	potential	2097:2105	arg1	applications					2107:2118	potential applications	2097:2118	potential applications in bone tissue engineering	2097:2145	Better cell viability and cell spreading morphology was achieved in lower alginate concentration scaffolds, which is relevant for potential applications in bone tissue engineering.					
31426033	3	121	theme	bioprinting	484:494	arg1	technique					496:504	a 3D bioprinting technique	479:504	a 3D bioprinting technique	479:504	In this study, we fabricated four different alginate concentration (0.8, 1.3, 1.8 and 2.3%alg) composite cell-laden porous scaffolds using a 3D bioprinting technique.					
31103928	7	0	theme	novel	985:989	arg1	scaffolds					1000:1008	novel bioglass scaffolds	985:1008	novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications	985:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	0	1	theme	plasma	86:91	arg1	sintering					93:101	robocasting and pressureless spark plasma sintering	51:101	robocasting and pressureless spark plasma sintering with graphene oxide	51:121	Reinforcing 13-93 bioglass scaffolds fabricated by robocasting and pressureless spark plasma sintering with graphene oxide.					
31103928	0	2	with	sintering	93:101	arg1	oxide					117:121	graphene oxide	108:121	graphene oxide	108:121	Reinforcing 13-93 bioglass scaffolds fabricated by robocasting and pressureless spark plasma sintering with graphene oxide.					
31103928	0	3	theme	graphene	108:115	arg1	oxide					117:121	graphene oxide	108:121	graphene oxide	108:121	Reinforcing 13-93 bioglass scaffolds fabricated by robocasting and pressureless spark plasma sintering with graphene oxide.					
31103928	7	4	theme	tissue	1049:1054	arg1	applications					1068:1079	load-bearing bone tissue engineering applications	1031:1079	load-bearing bone tissue engineering applications	1031:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	2	5	theme	%	285:285	arg1	content					287:293	0-4 vol% content	278:293	0-4 vol% content of GO platelets	278:309	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	3	6	theme	mechanical	531:540	arg1	performance					542:552	the mechanical performance	527:552	the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness	527:632	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31103928	7	7	from	Implications	935:946	arg1	fabrication					970:980	the fabrication	966:980	the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications	966:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	7	8	theme	engineering	1056:1066	arg1	applications					1068:1079	load-bearing bone tissue engineering applications	1031:1079	load-bearing bone tissue engineering applications	1031:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	2	9	theme	vol	282:284	arg1	content					287:293	0-4 vol% content	278:293	0-4 vol% content of GO platelets	278:309	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	7	10	theme	load-bearing	1031:1042	arg1	applications					1068:1079	load-bearing bone tissue engineering applications	1031:1079	load-bearing bone tissue engineering applications	1031:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	2	11	theme	0-4	278:280	arg1	vol					282:284	vol	282:284	vol	282:284	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	4	12	theme	Best	635:638	arg1	performance					640:650	Best performance	635:650	Best performance	635:650	Best performance was obtained for 2 vol.					
31103928	1	13	theme	graphene	170:177	arg1	oxide					179:183	graphene oxide	170:183	graphene oxide (GO)	170:188	13-93 bioglass (BG) scaffolds reinforced with graphene oxide (GO) were fabricated by robocasting (direct-ink-writing) technique.					
31103928	5	14	theme	strain	697:702	arg1	density					711:717	strain energy density	697:717	strain energy density (toughness) of the scaffolds by ∼894%	697:755	% GO, which increased strain energy density (toughness) of the scaffolds by ∼894%, and their compressive strength by ∼26%.					
31103928	5	14	theme	strain	697:702	arg1	toughness					720:728	toughness	720:728	toughness	720:728	% GO, which increased strain energy density (toughness) of the scaffolds by ∼894%, and their compressive strength by ∼26%.					
31103928	6	15	theme	higher	801:806	arg1	contents					808:815	higher contents	801:815	higher contents	801:815	At higher contents, agglomeration of the nanoplatelets and increased porosity significantly reduced the mechanical enhancement obtained.					
31103928	7	16	theme	bone	1044:1047	arg1	applications					1068:1079	load-bearing bone tissue engineering applications	1031:1079	load-bearing bone tissue engineering applications	1031:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	6	17	theme	increased	857:865	arg1	porosity					867:874	increased porosity	857:874	increased porosity	857:874	At higher contents, agglomeration of the nanoplatelets and increased porosity significantly reduced the mechanical enhancement obtained.					
31103928	0	18	theme	13-93	12:16	arg1	bioglass					18:25	13-93 bioglass	12:25	13-93 bioglass	12:25	Reinforcing 13-93 bioglass scaffolds fabricated by robocasting and pressureless spark plasma sintering with graphene oxide.					
31103928	5	19	theme	energy	704:709	arg1	density					711:717	strain energy density	697:717	strain energy density (toughness) of the scaffolds by ∼894%	697:755	% GO, which increased strain energy density (toughness) of the scaffolds by ∼894%, and their compressive strength by ∼26%.					
31103928	5	19	theme	energy	704:709	arg1	toughness					720:728	toughness	720:728	toughness	720:728	% GO, which increased strain energy density (toughness) of the scaffolds by ∼894%, and their compressive strength by ∼26%.					
31103928	2	20	theme	platelets	301:309	arg1	content					287:293	0-4 vol% content	278:293	0-4 vol% content of GO platelets	278:309	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	7	21	theme	bioglass	991:998	arg1	scaffolds					1000:1008	novel bioglass scaffolds	985:1008	novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications	985:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	2	22	theme	plasma	369:374	arg1	sintering					376:384	pressureless spark plasma sintering	350:384	pressureless spark plasma sintering at 650 °C	350:394	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	7	23	from	use	1024:1026	arg1	applications					1068:1079	load-bearing bone tissue engineering applications	1031:1079	load-bearing bone tissue engineering applications	1031:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	2	24	theme	spark	363:367	arg1	sintering					376:384	pressureless spark plasma sintering	350:384	pressureless spark plasma sintering at 650 °C	350:394	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	6	25	theme	mechanical	902:911	arg1	enhancement					913:923	the mechanical enhancement	898:923	the mechanical enhancement obtained	898:932	At higher contents, agglomeration of the nanoplatelets and increased porosity significantly reduced the mechanical enhancement obtained.					
31103928	3	26	dep	platelets	484:492	arg1	GO					481:482	GO	481:482	GO	481:482	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31103928	3	27	theme	platelets	484:492	arg1	addition					469:476	the addition	465:476	the addition of GO platelets up to a certain content	465:516	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31103928	7	28	theme	scaffolds	1000:1008	arg1	fabrication					970:980	the fabrication	966:980	the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications	966:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	5	29	theme	compressive	768:778	arg1	strength					780:787	their compressive strength	762:787	their compressive strength	762:787	% GO, which increased strain energy density (toughness) of the scaffolds by ∼894%, and their compressive strength by ∼26%.					
31103928	3	30	theme	strength	598:605	arg1	terms					589:593	terms	589:593	terms of strength and, especially, toughness	589:632	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31103928	3	31	theme	bioglass	567:574	arg1	scaffolds					576:584	the 13-93 bioglass scaffolds	557:584	the 13-93 bioglass scaffolds	557:584	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31103928	6	32	theme	porosity	867:874	arg1	agglomeration					818:830	agglomeration	818:830	agglomeration of the nanoplatelets and increased porosity	818:874	At higher contents, agglomeration of the nanoplatelets and increased porosity significantly reduced the mechanical enhancement obtained.					
31103928	5	33	theme	scaffolds	738:746	arg1	density					711:717	strain energy density	697:717	strain energy density (toughness) of the scaffolds by ∼894%	697:755	% GO, which increased strain energy density (toughness) of the scaffolds by ∼894%, and their compressive strength by ∼26%.					
31103928	5	33	theme	scaffolds	738:746	arg1	strength					780:787	their compressive strength	762:787	their compressive strength	762:787	% GO, which increased strain energy density (toughness) of the scaffolds by ∼894%, and their compressive strength by ∼26%.					
31103928	5	33	theme	scaffolds	738:746	arg1	toughness					720:728	toughness	720:728	toughness	720:728	% GO, which increased strain energy density (toughness) of the scaffolds by ∼894%, and their compressive strength by ∼26%.					
31103928	0	34	theme	robocasting	51:61	arg1	sintering					93:101	robocasting and pressureless spark plasma sintering	51:101	robocasting and pressureless spark plasma sintering with graphene oxide	51:121	Reinforcing 13-93 bioglass scaffolds fabricated by robocasting and pressureless spark plasma sintering with graphene oxide.					
31103928	2	35	with	scaffolds	263:271	arg1	content					287:293	0-4 vol% content	278:293	0-4 vol% content of GO platelets	278:309	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	6	36	theme	nanoplatelets	839:851	arg1	agglomeration					818:830	agglomeration	818:830	agglomeration of the nanoplatelets and increased porosity	818:874	At higher contents, agglomeration of the nanoplatelets and increased porosity significantly reduced the mechanical enhancement obtained.					
31103928	1	37	dep	oxide	179:183	arg1	GO					186:187	GO	186:187	GO	186:187	13-93 bioglass (BG) scaffolds reinforced with graphene oxide (GO) were fabricated by robocasting (direct-ink-writing) technique.					
31103928	1	38	theme	bioglass	130:137	arg1	scaffolds					144:152	13-93 bioglass (BG) scaffolds	124:152	13-93 bioglass (BG) scaffolds reinforced with graphene oxide (GO)	124:188	13-93 bioglass (BG) scaffolds reinforced with graphene oxide (GO) were fabricated by robocasting (direct-ink-writing) technique.					
31103928	0	39	dep	scaffolds	27:35	arg1	fabricated					37:46	fabricated	37:46	scaffolds fabricated by robocasting and pressureless spark plasma sintering with graphene oxide	27:121	Reinforcing 13-93 bioglass scaffolds fabricated by robocasting and pressureless spark plasma sintering with graphene oxide.					
31103928	7	40	theme	results	955:961	arg1	Implications					935:946	Implications	935:946	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications	935:1079	Implications of the results on the fabrication of novel bioglass scaffolds that may find use in load-bearing bone tissue engineering applications are discussed.					
31103928	2	41	dep	platelets	301:309	arg1	GO					298:299	GO	298:299	GO	298:299	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	1	42	theme	robocasting	209:219	arg1	technique					242:250	robocasting (direct-ink-writing) technique	209:250	robocasting (direct-ink-writing) technique	209:250	13-93 bioglass (BG) scaffolds reinforced with graphene oxide (GO) were fabricated by robocasting (direct-ink-writing) technique.					
31103928	2	43	theme	Composite	253:261	arg1	scaffolds					263:271	Composite scaffolds	253:271	Composite scaffolds with 0-4 vol% content of GO platelets	253:309	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	3	44	theme	bioglass	455:462	arg1	densification					434:446	densification	434:446	densification of the bioglass	434:462	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31103928	0	45	theme	spark	80:84	arg1	sintering					93:101	robocasting and pressureless spark plasma sintering	51:101	robocasting and pressureless spark plasma sintering with graphene oxide	51:121	Reinforcing 13-93 bioglass scaffolds fabricated by robocasting and pressureless spark plasma sintering with graphene oxide.					
31103928	3	46	theme	toughness	624:632	arg1	terms					589:593	terms	589:593	terms of strength and, especially, toughness	589:632	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31103928	3	47	theme	scaffolds	576:584	arg1	performance					542:552	the mechanical performance	527:552	the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness	527:632	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31103928	2	48	from	650 °C	389:394	arg1	sintering					376:384	pressureless spark plasma sintering	350:384	pressureless spark plasma sintering at 650 °C	350:394	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	2	49	theme	pressureless	350:361	arg1	sintering					376:384	pressureless spark plasma sintering	350:384	pressureless spark plasma sintering at 650 °C	350:394	Composite scaffolds with 0-4 vol% content of GO platelets were printed, and then consolidated by pressureless spark plasma sintering at 650 °C.					
31103928	3	50	from	performance	542:552	arg1	terms					589:593	terms	589:593	terms of strength and, especially, toughness	589:632	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31103928	1	51	theme	direct-ink-writing	222:239	arg1	technique					242:250	robocasting (direct-ink-writing) technique	209:250	robocasting (direct-ink-writing) technique	209:250	13-93 bioglass (BG) scaffolds reinforced with graphene oxide (GO) were fabricated by robocasting (direct-ink-writing) technique.					
31103928	0	52	theme	pressureless	67:78	arg1	sintering					93:101	robocasting and pressureless spark plasma sintering	51:101	robocasting and pressureless spark plasma sintering with graphene oxide	51:121	Reinforcing 13-93 bioglass scaffolds fabricated by robocasting and pressureless spark plasma sintering with graphene oxide.					
31103928	3	53	theme	certain	502:508	arg1	content					510:516	a certain content	500:516	a certain content	500:516	It was found that, despite hampering densification of the bioglass, the addition of GO platelets up to a certain content enhanced the mechanical performance of the 13-93 bioglass scaffolds in terms of strength and, especially, toughness.					
31720227	9	0	theme	metabolites	1600:1610	arg1	levels					1556:1561	elevated levels	1547:1561	elevated levels of both N-linked and O-linked glycan metabolites	1547:1610	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	10	1	theme	brain	1665:1669	arg1	samples					1681:1687	Both brain and urine samples	1660:1687	samples	1681:1687	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	11	2	theme	containing	2081:2090	arg1	glycolipids					2130:2140	glycolipids	2130:2140	glycolipids	2130:2140	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	2	theme	containing	2081:2090	arg1	glycans					2152:2158	N-linked glycans	2143:2158	N-linked glycans	2143:2158	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	2	theme	containing	2081:2090	arg1	glycans					2092:2098	many β-linked galactose containing glycans	2057:2098	many β-linked galactose containing glycans	2057:2098	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	2	theme	containing	2081:2090	arg1	glycans					2182:2188	various O-linked glycans	2165:2188	various O-linked glycans	2165:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	1	3	link	β-linked	260:267	arg1	galactose					269:277	β-linked galactose	260:277	β-linked galactose from the non-reducing end of glycans	260:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	3	4	theme	β-linked	556:563	arg1	galactose					565:573	β-linked galactose	556:573	β-linked galactose whose metabolites are substrates for β-galactosidase	556:626	In addition to GM1 and GA1, there are other glycoconjugates that contain β-linked galactose whose metabolites are substrates for β-galactosidase.					
31720227	5	5	dep	OBJECTIVE	818:826	arg1	study					836:840	this study	831:840	this study	831:840	OBJECTIVE In this study, we attempt to fully characterize the broad array of GLB1 substrates that require GLB1 for their lysosomal turnover.					
31720227	12	6	theme	therapies	2502:2510	arg1	efficacy					2483:2490	the efficacy	2479:2490	the efficacy of future therapies	2479:2510	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	11	7	theme	accumulation	1963:1974	arg1	disorder					1976:1983	a ganglioside accumulation disorder	1949:1983	a ganglioside accumulation disorder	1949:1983	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	7	theme	accumulation	1963:1974	arg1	deficiency					1924:1933	GLB1 deficiency	1919:1933	GLB1 deficiency	1919:1933	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	1	8	theme	GLB1	185:188	arg1	gene					190:193	the GLB1 gene	181:193	the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans	181:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	1	8	theme	GLB1	185:188	arg1	β-galactosidase					208:222	β-galactosidase	208:222	β-galactosidase	208:222	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	9	9	link	O-linked	1584:1591	arg1	metabolites					1600:1610	both N-linked and O-linked glycan metabolites	1566:1610	both N-linked and O-linked glycan metabolites	1566:1610	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	9	10	theme	tissues	1638:1644	arg1	number					1617:1622	a number	1615:1622	a number of peripheral tissues	1615:1644	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	1	11	from	end	301:303	arg1	galactose					269:277	β-linked galactose	260:277	β-linked galactose from the non-reducing end of glycans	260:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	10	12	theme	large	1738:1742	arg1	increases					1744:1752	large increases	1738:1752	large increases in steady state levels for the same glycan metabolites	1738:1807	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	11	13	theme	glycoconjugates	2104:2118	arg1	representatives					2038:2052	representatives	2038:2052	representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans	2038:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	9	14	link	N-linked	1571:1578	arg1	metabolites					1600:1610	both N-linked and O-linked glycan metabolites	1566:1610	both N-linked and O-linked glycan metabolites	1566:1610	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	2	15	from	accumulation	376:387	arg1	patients					455:462	β-galactosidase deficient patients	429:462	β-galactosidase deficient patients	429:462	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	2	15	from	accumulation	376:387	arg1	models					475:480	animal models	468:480	animal models	468:480	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	6	16	theme	terminal	1165:1172	arg1	galactose					1186:1194	terminal beta-linked galactose	1165:1194	terminal beta-linked galactose	1165:1194	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	6	17	theme	tandem	973:978	arg1	spectrometry					985:996	tandem mass spectrometry	973:996	tandem mass spectrometry	973:996	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	8	18	theme	metabolites	1428:1438	arg1	levels					1405:1410	the levels	1401:1410	the levels of these glycan metabolites	1401:1438	In the brain of GLB1 null mice, the levels of these glycan metabolites increased along with those of both GM1 and GA1 as a function of age.					
31720227	11	19	link	N-linked	2143:2150	arg1	glycans					2152:2158	N-linked glycans	2143:2158	N-linked glycans	2143:2158	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	4	20	theme	glycan	663:668	arg1	structures					670:679	N-linked glycan structures	654:679	N-linked glycan structures that have galactose at their non-reducing end	654:725	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	10	21	theme	state	1764:1768	arg1	levels					1770:1775	steady state levels	1757:1775	steady state levels for the same glycan metabolites	1757:1807	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	11	22	theme	β-linked	2062:2069	arg1	glycolipids					2130:2140	glycolipids	2130:2140	glycolipids	2130:2140	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	22	theme	β-linked	2062:2069	arg1	glycans					2152:2158	N-linked glycans	2143:2158	N-linked glycans	2143:2158	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	22	theme	β-linked	2062:2069	arg1	glycans					2092:2098	many β-linked galactose containing glycans	2057:2098	many β-linked galactose containing glycans	2057:2098	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	22	theme	β-linked	2062:2069	arg1	glycans					2182:2188	various O-linked glycans	2165:2188	various O-linked glycans	2165:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	1	23	theme	genetic	136:142	arg1	disorder					144:151	a rare autosomal recessive genetic disorder	109:151	a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans	109:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	1	23	theme	genetic	136:142	arg1	gangliosidosis					91:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	12	24	theme	severe	2319:2324	arg1	disorder					2326:2333	this severe disorder	2314:2333	this severe disorder	2314:2333	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	6	25	dep	glycolipids	1113:1123	arg1	GA1					1134:1136	GA1	1134:1136	GA1	1134:1136	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	6	25	dep	glycolipids	1113:1123	arg1	GM1					1126:1128	GM1	1126:1128	GM1	1126:1128	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	6	25	dep	glycolipids	1113:1123	arg1	glycolipids					1113:1123	glycolipids	1113:1123	glycolipids (GM1 and GA1)	1113:1137	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	10	26	theme	same	1785:1788	arg1	metabolites					1797:1807	the same glycan metabolites	1781:1807	the same glycan metabolites	1781:1807	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	5	27	theme	lysosomal	939:947	arg1	turnover					949:956	their lysosomal turnover	933:956	their lysosomal turnover	933:956	OBJECTIVE In this study, we attempt to fully characterize the broad array of GLB1 substrates that require GLB1 for their lysosomal turnover.					
31720227	6	28	theme	mass	1056:1059	arg1	spectrometry					1061:1072	data-dependent mass spectrometry	1041:1072	data-dependent mass spectrometry	1041:1072	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	6	29	with	labeling	1027:1034	arg1	spectrometry					1061:1072	data-dependent mass spectrometry	1041:1072	data-dependent mass spectrometry	1041:1072	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	5	30	theme	substrates	900:909	arg1	array					886:890	the broad array	876:890	the broad array of GLB1 substrates that require GLB1 for their lysosomal turnover	876:956	OBJECTIVE In this study, we attempt to fully characterize the broad array of GLB1 substrates that require GLB1 for their lysosomal turnover.					
31720227	12	31	theme	informative	2430:2440	arg1	metabolites					2350:2360	metabolites	2350:2360	metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies	2350:2510	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	12	31	theme	informative	2430:2440	arg1	biomarkers					2450:2459	informative disease biomarkers	2430:2459	informative disease biomarkers	2430:2459	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	9	32	dep	tissue	1530:1535	arg1	addition					1512:1519	addition	1512:1519	addition	1512:1519	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	7	33	theme	core	1236:1239	arg1	core					1236:1239	core 1	1236:1241	core 1	1236:1241	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	33	theme	core	1236:1239	arg1	set					1229:1231	a novel set	1221:1231	a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease	1221:1366	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	33	theme	core	1236:1239	arg1	metabolites					1265:1275	2 O-linked glycan metabolites	1247:1275	2 O-linked glycan metabolites	1247:1275	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	10	34	from	increases	1744:1752	arg1	levels					1770:1775	steady state levels	1757:1775	steady state levels for the same glycan metabolites	1757:1807	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	6	35	theme	isotope	1019:1025	arg1	labeling					1027:1034	glycan reductive isotope labeling	1002:1034	glycan reductive isotope labeling	1002:1034	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	12	36	theme	β-galactosidase	2210:2224	arg1	substrates					2226:2235	all β-galactosidase substrates	2206:2235	all β-galactosidase substrates that accumulate when this enzyme is deficient	2206:2281	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	10	37	theme	GM1	1700:1702	arg1	patients					1719:1726	human GM1 gangliosidosis patients	1694:1726	human GM1 gangliosidosis patients	1694:1726	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	1	38	theme	INTRODUCTION	74:85	arg1	disorder					144:151	a rare autosomal recessive genetic disorder	109:151	a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans	109:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	1	38	theme	INTRODUCTION	74:85	arg1	gangliosidosis					91:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	6	39	theme	glycan	1002:1007	arg1	labeling					1027:1034	glycan reductive isotope labeling	1002:1034	glycan reductive isotope labeling	1002:1034	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	2	40	from	models	475:480	arg1	accumulation					376:387	the lysosomal accumulation	362:387	the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models	362:480	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	4	41	theme	biological	799:808	arg1	fluids					810:815	biological fluids	799:815	biological fluids	799:815	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	2	42	theme	asialo	404:409	arg1	GA1					422:424	its asialo derivative GA1	400:424	its asialo derivative GA1	400:424	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	9	43	theme	brain	1524:1528	arg1	tissue					1530:1535	brain tissue	1524:1535	brain tissue	1524:1535	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	2	44	theme	animal	468:473	arg1	models					475:480	animal models	468:480	animal models	468:480	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	8	45	theme	age	1504:1506	arg1	function					1492:1499	a function	1490:1499	a function of age	1490:1506	In the brain of GLB1 null mice, the levels of these glycan metabolites increased along with those of both GM1 and GA1 as a function of age.					
31720227	4	46	theme	gangliosidosis	764:777	arg1	tissues					787:793	GM1 gangliosidosis patient tissues	760:793	GM1 gangliosidosis patient tissues	760:793	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	1	47	theme	non-reducing	288:299	arg1	end					301:303	the non-reducing end	284:303	the non-reducing end of glycans	284:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	7	48	theme	series	1334:1339	arg1	many					1278:1281	many	1278:1281	many	1278:1281	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	48	theme	series	1334:1339	arg1	metabolites					1265:1275	2 O-linked glycan metabolites	1247:1275	2 O-linked glycan metabolites	1247:1275	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	48	theme	series	1334:1339	arg1	part					1296:1299	part	1296:1299	part of structurally-related isobaric series that accumulate in disease	1296:1366	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	8	49	theme	GLB1	1385:1388	arg1	mice					1395:1398	GLB1 null mice	1385:1398	GLB1 null mice	1385:1398	In the brain of GLB1 null mice, the levels of these glycan metabolites increased along with those of both GM1 and GA1 as a function of age.					
31720227	2	50	theme	β-galactosidase	429:443	arg1	patients					455:462	β-galactosidase deficient patients	429:462	β-galactosidase deficient patients	429:462	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	0	51	theme	glycan	20:25	arg1	substrates					27:36	glycan substrates	20:36	glycan substrates	20:36	Characterization of glycan substrates accumulating in GM1 Gangliosidosis.					
31720227	11	52	theme	ganglioside	1951:1961	arg1	disorder					1976:1983	a ganglioside accumulation disorder	1949:1983	a ganglioside accumulation disorder	1949:1983	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	52	theme	ganglioside	1951:1961	arg1	deficiency					1924:1933	GLB1 deficiency	1919:1933	GLB1 deficiency	1919:1933	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	1	53	theme	autosomal	116:124	arg1	disorder					144:151	a rare autosomal recessive genetic disorder	109:151	a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans	109:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	1	53	theme	autosomal	116:124	arg1	gangliosidosis					91:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	7	54	theme	structurally-related	1304:1323	arg1	series					1334:1339	structurally-related isobaric series	1304:1339	structurally-related isobaric series that accumulate in disease	1304:1366	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	8	55	theme	mice	1395:1398	arg1	brain					1376:1380	the brain	1372:1380	the brain of GLB1 null mice	1372:1398	In the brain of GLB1 null mice, the levels of these glycan metabolites increased along with those of both GM1 and GA1 as a function of age.					
31720227	2	56	theme	GA1	422:424	arg1	accumulation					376:387	the lysosomal accumulation	362:387	the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models	362:480	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	6	57	with	glycolipids	1113:1123	arg1	galactose					1186:1194	terminal beta-linked galactose	1165:1194	terminal beta-linked galactose	1165:1194	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	11	58	theme	O-linked	2173:2180	arg1	glycans					2182:2188	various O-linked glycans	2165:2188	various O-linked glycans	2165:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	6	59	link	N-linked	1143:1150	arg1	glycans					1152:1158	N-linked glycans	1143:1158	N-linked glycans with terminal beta-linked galactose	1143:1194	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	0	60	theme	GM1	54:56	arg1	Gangliosidosis					58:71	GM1 Gangliosidosis	54:71	GM1 Gangliosidosis	54:71	Characterization of glycan substrates accumulating in GM1 Gangliosidosis.					
31720227	11	61	theme	N-linked	2143:2150	arg1	glycans					2152:2158	N-linked glycans	2143:2158	N-linked glycans	2143:2158	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	6	62	theme	N-linked	1143:1150	arg1	glycans					1152:1158	N-linked glycans	1143:1158	N-linked glycans with terminal beta-linked galactose	1143:1194	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	3	63	contain	contain	548:554	arg1	glycoconjugates					527:541	other glycoconjugates	521:541	other glycoconjugates that contain β-linked galactose whose metabolites are substrates for β-galactosidase	521:626	In addition to GM1 and GA1, there are other glycoconjugates that contain β-linked galactose whose metabolites are substrates for β-galactosidase.					
31720227	3	63	contain	contain	548:554	arg2	galactose					565:573	β-linked galactose	556:573	β-linked galactose whose metabolites are substrates for β-galactosidase	556:626	In addition to GM1 and GA1, there are other glycoconjugates that contain β-linked galactose whose metabolites are substrates for β-galactosidase.					
31720227	2	64	theme	enzyme	346:351	arg1	Deficiency					317:326	Deficiency	317:326	Deficiency of this catabolic enzyme	317:351	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	1	65	theme	lysosomal	227:235	arg1	β-galactosidase					208:222	β-galactosidase	208:222	β-galactosidase	208:222	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	1	65	theme	lysosomal	227:235	arg1	hydrolase					237:245	a lysosomal hydrolase	225:245	a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans	225:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	11	66	dep	CONCLUSIONS	1879:1889	arg1	illustrate					1903:1912	illustrate	1903:1912	illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans	1903:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	2	67	from	GA1	422:424	arg1	patients					455:462	β-galactosidase deficient patients	429:462	β-galactosidase deficient patients	429:462	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	2	67	from	GA1	422:424	arg1	models					475:480	animal models	468:480	animal models	468:480	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	12	68	theme	disease	2400:2406	arg1	aspects					2385:2391	certain aspects	2377:2391	certain aspects of the disease	2377:2406	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	8	69	theme	glycan	1421:1426	arg1	metabolites					1428:1438	these glycan metabolites	1415:1438	these glycan metabolites	1415:1438	In the brain of GLB1 null mice, the levels of these glycan metabolites increased along with those of both GM1 and GA1 as a function of age.					
31720227	5	70	theme	broad	880:884	arg1	array					886:890	the broad array	876:890	the broad array of GLB1 substrates that require GLB1 for their lysosomal turnover	876:956	OBJECTIVE In this study, we attempt to fully characterize the broad array of GLB1 substrates that require GLB1 for their lysosomal turnover.					
31720227	9	71	theme	glycan	1593:1598	arg1	metabolites					1600:1610	both N-linked and O-linked glycan metabolites	1566:1610	both N-linked and O-linked glycan metabolites	1566:1610	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	7	72	theme	metabolites	1265:1275	arg1	many					1278:1281	many	1278:1281	many	1278:1281	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	72	theme	metabolites	1265:1275	arg1	set					1229:1231	a novel set	1221:1231	a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease	1221:1366	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	72	theme	metabolites	1265:1275	arg1	metabolites					1265:1275	2 O-linked glycan metabolites	1247:1275	2 O-linked glycan metabolites	1247:1275	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	72	theme	metabolites	1265:1275	arg1	part					1296:1299	part	1296:1299	part of structurally-related isobaric series that accumulate in disease	1296:1366	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	72	theme	metabolites	1265:1275	arg1	core					1236:1239	core 1	1236:1241	core 1	1236:1241	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	2	73	theme	lysosomal	366:374	arg1	accumulation					376:387	the lysosomal accumulation	362:387	the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models	362:480	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	1	74	theme	β-linked	260:267	arg1	galactose					269:277	β-linked galactose	260:277	β-linked galactose from the non-reducing end of glycans	260:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	6	75	with	glycans	1152:1158	arg1	galactose					1186:1194	terminal beta-linked galactose	1165:1194	terminal beta-linked galactose	1165:1194	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	10	76	with	correlation	1830:1840	arg1	disease					1852:1858	this disease	1847:1858	this disease	1847:1858	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	7	77	theme	O-linked	1249:1256	arg1	metabolites					1265:1275	2 O-linked glycan metabolites	1247:1275	2 O-linked glycan metabolites	1247:1275	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	12	78	theme	certain	2377:2383	arg1	aspects					2385:2391	certain aspects	2377:2391	certain aspects of the disease	2377:2406	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	10	79	theme	gangliosidosis	1704:1717	arg1	patients					1719:1726	human GM1 gangliosidosis patients	1694:1726	human GM1 gangliosidosis patients	1694:1726	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	11	80	theme	glycans	2092:2098	arg1	representatives					2038:2052	representatives	2038:2052	representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans	2038:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	10	81	theme	human	1694:1698	arg1	patients					1719:1726	human GM1 gangliosidosis patients	1694:1726	human GM1 gangliosidosis patients	1694:1726	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	9	82	theme	peripheral	1627:1636	arg1	tissues					1638:1644	peripheral tissues	1627:1644	peripheral tissues	1627:1644	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	6	83	with	spectrometry	985:996	arg1	spectrometry					1061:1072	data-dependent mass spectrometry	1041:1072	data-dependent mass spectrometry	1041:1072	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	4	84	theme	structures	670:679	arg1	number					644:649	a number	642:649	a number of N-linked glycan structures that have galactose at their non-reducing end	642:725	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	1	85	theme	gene	190:193	arg1	disruption					167:176	the disruption	163:176	the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans	163:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	6	86	theme	beta-linked	1174:1184	arg1	galactose					1186:1194	terminal beta-linked galactose	1165:1194	terminal beta-linked galactose	1165:1194	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	11	87	theme	broad	2000:2004	arg1	oligosaccharidosis					2006:2023	a broad oligosaccharidosis	1998:2023	a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans	1998:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	6	88	theme	mass	980:983	arg1	spectrometry					985:996	tandem mass spectrometry	973:996	tandem mass spectrometry	973:996	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	7	89	link	O-linked	1249:1256	arg1	metabolites					1265:1275	2 O-linked glycan metabolites	1247:1275	2 O-linked glycan metabolites	1247:1275	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	12	90	theme	future	2495:2500	arg1	therapies					2502:2510	future therapies	2495:2510	future therapies	2495:2510	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	11	91	link	O-linked	2173:2180	arg1	glycans					2182:2188	various O-linked glycans	2165:2188	various O-linked glycans	2165:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	4	92	theme	N-linked	654:661	arg1	structures					670:679	N-linked glycan structures	654:679	N-linked glycan structures that have galactose at their non-reducing end	654:725	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	10	93	theme	steady	1757:1762	arg1	levels					1770:1775	steady state levels	1757:1775	steady state levels for the same glycan metabolites	1757:1807	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	1	94	theme	recessive	126:134	arg1	disorder					144:151	a rare autosomal recessive genetic disorder	109:151	a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans	109:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	1	94	theme	recessive	126:134	arg1	gangliosidosis					91:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	10	95	theme	urine	1675:1679	arg1	samples					1681:1687	Both brain and urine samples	1660:1687	samples	1681:1687	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	11	96	theme	galactose	2071:2079	arg1	glycolipids					2130:2140	glycolipids	2130:2140	glycolipids	2130:2140	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	96	theme	galactose	2071:2079	arg1	glycans					2152:2158	N-linked glycans	2143:2158	N-linked glycans	2143:2158	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	96	theme	galactose	2071:2079	arg1	glycans					2092:2098	many β-linked galactose containing glycans	2057:2098	many β-linked galactose containing glycans	2057:2098	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	96	theme	galactose	2071:2079	arg1	glycans					2182:2188	various O-linked glycans	2165:2188	various O-linked glycans	2165:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	12	97	theme	disorder	2326:2333	arg1	understanding					2297:2309	our understanding	2293:2309	our understanding of this severe disorder	2293:2333	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	10	98	theme	glycan	1790:1795	arg1	metabolites					1797:1807	the same glycan metabolites	1781:1807	the same glycan metabolites	1781:1807	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	11	99	theme	many	2057:2060	arg1	glycolipids					2130:2140	glycolipids	2130:2140	glycolipids	2130:2140	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	99	theme	many	2057:2060	arg1	glycans					2152:2158	N-linked glycans	2143:2158	N-linked glycans	2143:2158	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	99	theme	many	2057:2060	arg1	glycans					2092:2098	many β-linked galactose containing glycans	2057:2098	many β-linked galactose containing glycans	2057:2098	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	99	theme	many	2057:2060	arg1	glycans					2182:2188	various O-linked glycans	2165:2188	various O-linked glycans	2165:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	4	100	link	N-linked	654:661	arg1	structures					670:679	N-linked glycan structures	654:679	N-linked glycan structures that have galactose at their non-reducing end	654:725	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	11	101	link	β-linked	2062:2069	arg1	glycolipids					2130:2140	glycolipids	2130:2140	glycolipids	2130:2140	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	101	link	β-linked	2062:2069	arg1	glycans					2152:2158	N-linked glycans	2143:2158	N-linked glycans	2143:2158	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	101	link	β-linked	2062:2069	arg1	glycans					2092:2098	many β-linked galactose containing glycans	2057:2098	many β-linked galactose containing glycans	2057:2098	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	101	link	β-linked	2062:2069	arg1	glycans					2182:2188	various O-linked glycans	2165:2188	various O-linked glycans	2165:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	5	102	theme	GLB1	895:898	arg1	substrates					900:909	GLB1 substrates	895:909	GLB1 substrates that require GLB1 for their lysosomal turnover	895:956	OBJECTIVE In this study, we attempt to fully characterize the broad array of GLB1 substrates that require GLB1 for their lysosomal turnover.					
31720227	6	103	theme	data-dependent	1041:1054	arg1	spectrometry					1061:1072	data-dependent mass spectrometry	1041:1072	data-dependent mass spectrometry	1041:1072	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	2	104	from	patients	455:462	arg1	accumulation					376:387	the lysosomal accumulation	362:387	the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models	362:480	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	10	105	from	patients	1719:1726	arg1	samples					1681:1687	Both brain and urine samples	1660:1687	samples	1681:1687	Both brain and urine samples from human GM1 gangliosidosis patients exhibited large increases in steady state levels for the same glycan metabolites, demonstrating their correlation with this disease in humans as well.					
31720227	4	106	theme	non-reducing	710:721	arg1	end					723:725	their non-reducing end	704:725	their non-reducing end	704:725	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	7	107	theme	novel	1223:1227	arg1	core					1236:1239	core 1	1236:1241	core 1	1236:1241	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	107	theme	novel	1223:1227	arg1	set					1229:1231	a novel set	1221:1231	a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease	1221:1366	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	7	107	theme	novel	1223:1227	arg1	metabolites					1265:1275	2 O-linked glycan metabolites	1247:1275	2 O-linked glycan metabolites	1247:1275	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	6	108	theme	reductive	1009:1017	arg1	labeling					1027:1034	glycan reductive isotope labeling	1002:1034	glycan reductive isotope labeling	1002:1034	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	4	109	theme	patient	779:785	arg1	tissues					787:793	GM1 gangliosidosis patient tissues	760:793	GM1 gangliosidosis patient tissues	760:793	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	6	110	link	beta-linked	1174:1184	arg1	galactose					1186:1194	terminal beta-linked galactose	1165:1194	terminal beta-linked galactose	1165:1194	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	1	111	theme	GM1	87:89	arg1	disorder					144:151	a rare autosomal recessive genetic disorder	109:151	a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans	109:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	1	111	theme	GM1	87:89	arg1	gangliosidosis					91:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	6	112	theme	glycolipids	1113:1123	arg1	accumulation					1097:1108	the accumulation	1093:1108	the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose	1093:1194	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	3	113	link	β-linked	556:563	arg1	galactose					565:573	β-linked galactose	556:573	β-linked galactose whose metabolites are substrates for β-galactosidase	556:626	In addition to GM1 and GA1, there are other glycoconjugates that contain β-linked galactose whose metabolites are substrates for β-galactosidase.					
31720227	7	114	theme	isobaric	1325:1332	arg1	series					1334:1339	structurally-related isobaric series	1304:1339	structurally-related isobaric series that accumulate in disease	1304:1366	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	11	115	theme	various	2165:2171	arg1	glycans					2182:2188	various O-linked glycans	2165:2188	various O-linked glycans	2165:2188	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	2	116	theme	GM1	392:394	arg1	accumulation					376:387	the lysosomal accumulation	362:387	the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models	362:480	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	0	117	theme	substrates	27:36	arg1	Characterization					0:15	Characterization	0:15	Characterization of glycan substrates	0:36	Characterization of glycan substrates accumulating in GM1 Gangliosidosis.					
31720227	1	118	theme	rare	111:114	arg1	disorder					144:151	a rare autosomal recessive genetic disorder	109:151	a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans	109:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	1	118	theme	rare	111:114	arg1	gangliosidosis					91:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis	74:104	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	11	119	theme	GLB1	1919:1922	arg1	disorder					1976:1983	a ganglioside accumulation disorder	1949:1983	a ganglioside accumulation disorder	1949:1983	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	11	119	theme	GLB1	1919:1922	arg1	deficiency					1924:1933	GLB1 deficiency	1919:1933	GLB1 deficiency	1919:1933	CONCLUSIONS Our studies illustrate that GLB1 deficiency is not purely a ganglioside accumulation disorder, but instead a broad oligosaccharidosis that include representatives of many β-linked galactose containing glycans and glycoconjugates including glycolipids, N-linked glycans, and various O-linked glycans.					
31720227	2	120	theme	deficient	445:453	arg1	patients					455:462	β-galactosidase deficient patients	429:462	β-galactosidase deficient patients	429:462	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	4	121	theme	GM1	760:762	arg1	tissues					787:793	GM1 gangliosidosis patient tissues	760:793	GM1 gangliosidosis patient tissues	760:793	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	1	122	theme	glycans	308:314	arg1	end					301:303	the non-reducing end	284:303	the non-reducing end of glycans	284:314	INTRODUCTION GM1 gangliosidosis is a rare autosomal recessive genetic disorder caused by the disruption of the GLB1 gene that encodes β-galactosidase, a lysosomal hydrolase that removes β-linked galactose from the non-reducing end of glycans.					
31720227	9	123	theme	elevated	1547:1554	arg1	levels					1556:1561	elevated levels	1547:1561	elevated levels of both N-linked and O-linked glycan metabolites	1547:1610	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	8	124	theme	null	1390:1393	arg1	mice					1395:1398	GLB1 null mice	1385:1398	GLB1 null mice	1385:1398	In the brain of GLB1 null mice, the levels of these glycan metabolites increased along with those of both GM1 and GA1 as a function of age.					
31720227	2	125	theme	derivative	411:420	arg1	GA1					422:424	its asialo derivative GA1	400:424	its asialo derivative GA1	400:424	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	2	126	from	GM1	392:394	arg1	patients					455:462	β-galactosidase deficient patients	429:462	β-galactosidase deficient patients	429:462	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	2	126	from	GM1	392:394	arg1	models					475:480	animal models	468:480	animal models	468:480	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	6	127	theme	glycans	1152:1158	arg1	accumulation					1097:1108	the accumulation	1093:1108	the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose	1093:1194	RESULTS Using tandem mass spectrometry and glycan reductive isotope labeling with data-dependent mass spectrometry, we have confirmed the accumulation of glycolipids (GM1 and GA1) and N-linked glycans with terminal beta-linked galactose.					
31720227	12	128	theme	disease	2442:2448	arg1	metabolites					2350:2360	metabolites	2350:2360	metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies	2350:2510	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	12	128	theme	disease	2442:2448	arg1	biomarkers					2450:2459	informative disease biomarkers	2430:2459	informative disease biomarkers	2430:2459	Accounting for all β-galactosidase substrates that accumulate when this enzyme is deficient increases our understanding of this severe disorder by identifying metabolites that may drive certain aspects of the disease and may also serve as informative disease biomarkers to fully evaluate the efficacy of future therapies.					
31720227	4	129	contain	have	686:689	arg2	galactose					691:699	galactose	691:699	galactose	691:699	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	4	129	contain	have	686:689	arg1	structures					670:679	N-linked glycan structures	654:679	N-linked glycan structures that have galactose at their non-reducing end	654:725	For example, a number of N-linked glycan structures that have galactose at their non-reducing end have been shown to accumulate in GM1 gangliosidosis patient tissues and biological fluids.					
31720227	9	130	theme	N-linked	1571:1578	arg1	metabolites					1600:1610	both N-linked and O-linked glycan metabolites	1566:1610	both N-linked and O-linked glycan metabolites	1566:1610	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	2	131	theme	catabolic	336:344	arg1	enzyme					346:351	this catabolic enzyme	331:351	this catabolic enzyme	331:351	Deficiency of this catabolic enzyme leads to the lysosomal accumulation of GM1 and its asialo derivative GA1 in β-galactosidase deficient patients and animal models.					
31720227	9	132	theme	O-linked	1584:1591	arg1	metabolites					1600:1610	both N-linked and O-linked glycan metabolites	1566:1610	both N-linked and O-linked glycan metabolites	1566:1610	In addition to brain tissue, we found elevated levels of both N-linked and O-linked glycan metabolites in a number of peripheral tissues and in urine.					
31720227	7	133	theme	glycan	1258:1263	arg1	metabolites					1265:1275	2 O-linked glycan metabolites	1247:1275	2 O-linked glycan metabolites	1247:1275	We have also discovered a novel set of core 1 and 2 O-linked glycan metabolites, many of which are part of structurally-related isobaric series that accumulate in disease.					
31720227	3	134	theme	other	521:525	arg1	glycoconjugates					527:541	other glycoconjugates	521:541	other glycoconjugates that contain β-linked galactose whose metabolites are substrates for β-galactosidase	521:626	In addition to GM1 and GA1, there are other glycoconjugates that contain β-linked galactose whose metabolites are substrates for β-galactosidase.					
31280522	3	0	theme	statistical	262:272	arg1	approach					274:281	a statistical approach	260:281	a statistical approach	260:281	FBL-2 was studied using a statistical approach.					
31280522	6	1	dep	acid	891:894	arg1	g/l					903:905	105.30 g/l	896:905	105.30 g/l	896:905	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	4	2	theme	sources	357:363	arg1	effect					340:345	the effect	336:345	the effect of carbon sources and nitrogen sources on γ-PGA production	336:404	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	7	3	theme	γ-PGA	1015:1019	arg1	production					1021:1030	γ-PGA production	1015:1030	γ-PGA production	1015:1030	The optimized medium was validated experimentally, and γ-PGA production increased significantly from 3.59 g/l (0.33 g/l/h) to 44.04 g/l (3.67 g/l/h) when strain FBL-2 was cultivated under the optimal medium developed by the statistical approach, as compared to non-optimized medium.					
31280522	5	4	theme	key	684:686	arg1	nutrients					688:696	the key nutrients	680:696	the key nutrients	680:696	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	5	theme	surface	542:548	arg1	methodology					550:560	response surface methodology	533:560	response surface methodology with central composite design (CCD)	533:596	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	4	6	theme	carbon	350:355	arg1	sources					357:363	carbon sources	350:363	carbon sources	350:363	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	6	7	theme	extract	914:920	arg1	g/l					928:930	yeast extract 13.25 g/l	908:930	yeast extract 13.25 g/l	908:930	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	7	theme	extract	914:920	arg1	acid					891:894	L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l,	880:931	acid	891:894	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	5	8	theme	nutrients	688:696	arg1	concentrations					662:675	the optimum concentrations	650:675	the optimum concentrations of the key nutrients	650:696	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	6	9	theme	components	835:844	arg1	components					835:844	significant medium components	816:844	significant medium components	816:844	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	9	theme	components	835:844	arg1	g/l					875:877	sucrose 51.73 g/l	861:877	sucrose 51.73 g/l	861:877	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	9	theme	components	835:844	arg1	amount					806:811	The optimum amount	794:811	The optimum amount of significant medium components	794:844	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	5	10	theme	mathematical	619:630	arg1	model					632:636	a mathematical model	617:636	a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production	617:791	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	4	11	theme	nitrogen	369:376	arg1	sources					378:384	nitrogen sources	369:384	nitrogen sources	369:384	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	7	12	theme	optimal	1152:1158	arg1	medium					1160:1165	the optimal medium	1148:1165	the optimal medium developed by the statistical approach	1148:1203	The optimized medium was validated experimentally, and γ-PGA production increased significantly from 3.59 g/l (0.33 g/l/h) to 44.04 g/l (3.67 g/l/h) when strain FBL-2 was cultivated under the optimal medium developed by the statistical approach, as compared to non-optimized medium.					
31280522	6	13	theme	yeast	908:912	arg1	g/l					928:930	yeast extract 13.25 g/l	908:930	yeast extract 13.25 g/l	908:930	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	13	theme	yeast	908:912	arg1	acid					891:894	L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l,	880:931	acid	891:894	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	14	theme	medium	828:833	arg1	components					835:844	significant medium components	816:844	significant medium components	816:844	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	4	15	used	used	316:319	arg2	method					305:310	One-factor-at-a-time method	284:310	One-factor-at-a-time method	284:310	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	4	16	theme	significant	442:452	arg1	nutrients					454:462	the most significant nutrients	433:462	the most significant nutrients affecting the yield of γ-PGA	433:491	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	5	17	theme	yeast	725:729	arg1	extract					731:737	yeast extract	725:737	yeast extract	725:737	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	18	theme	response	533:540	arg1	methodology					550:560	response surface methodology	533:560	response surface methodology with central composite design (CCD)	533:596	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	2	19	theme	Bacillus	223:230	arg1	sp					232:233	Bacillus sp	223:233	Bacillus sp	223:233	In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp.					
31280522	7	20	theme	non-optimized	1221:1233	arg1	medium					1235:1240	non-optimized medium	1221:1240	non-optimized medium	1221:1240	The optimized medium was validated experimentally, and γ-PGA production increased significantly from 3.59 g/l (0.33 g/l/h) to 44.04 g/l (3.67 g/l/h) when strain FBL-2 was cultivated under the optimal medium developed by the statistical approach, as compared to non-optimized medium.					
31280522	5	21	theme	citric	744:749	arg1	acid					751:754	citric acid	744:754	citric acid	744:754	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	6	22	dep	acid	944:947	arg1	g/l					955:957	10.04 g/l	949:957	citric acid 10.04 g/l	937:957	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	0	23	theme	Bacillus	49:56	arg1	sp					58:59	Bacillus sp	49:59	Bacillus sp	49:59	Optimized Production of Poly(γ-Glutamic acid) By Bacillus sp.					
31280522	1	24	theme	FBL-2	62:66	arg1	Methodology					93:103	FBL-2 through Response Surface Methodology	62:103	FBL-2 through Response Surface Methodology	62:103	FBL-2 through Response Surface Methodology Using Central Composite Design.					
31280522	5	25	used	used	602:605	arg2	methodology					550:560	response surface methodology	533:560	response surface methodology with central composite design (CCD)	533:596	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	26	with	methodology	550:560	arg1	CCD					593:595	CCD	593:595	CCD	593:595	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	26	with	methodology	550:560	arg1	design					585:590	central composite design	567:590	central composite design (CCD)	567:596	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	1	27	theme	through	68:74	arg1	Methodology					93:103	FBL-2 through Response Surface Methodology	62:103	FBL-2 through Response Surface Methodology	62:103	FBL-2 through Response Surface Methodology Using Central Composite Design.					
31280522	0	28	theme	Poly	24:27	arg1	Production					10:19	Production	10:19	Production of Poly(γ-Glutamic acid)	10:44	Optimized Production of Poly(γ-Glutamic acid) By Bacillus sp.					
31280522	5	29	theme	optimum	654:660	arg1	concentrations					662:675	the optimum concentrations	650:675	the optimum concentrations of the key nutrients	650:696	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	1	30	theme	Response	76:83	arg1	Methodology					93:103	FBL-2 through Response Surface Methodology	62:103	FBL-2 through Response Surface Methodology	62:103	FBL-2 through Response Surface Methodology Using Central Composite Design.					
31280522	5	31	theme	central	567:573	arg1	CCD					593:595	CCD	593:595	CCD	593:595	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	31	theme	central	567:573	arg1	design					585:590	central composite design	567:590	central composite design (CCD)	567:596	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	4	32	from	effect	340:345	arg1	production					395:404	γ-PGA production	389:404	γ-PGA production	389:404	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	1	33	theme	Surface	85:91	arg1	Methodology					93:103	FBL-2 through Response Surface Methodology	62:103	FBL-2 through Response Surface Methodology	62:103	FBL-2 through Response Surface Methodology Using Central Composite Design.					
31280522	0	34	theme	γ-Glutamic	29:38	arg1	Poly					24:27	Poly	24:27	Poly(γ-Glutamic acid)	24:44	Optimized Production of Poly(γ-Glutamic acid) By Bacillus sp.					
31280522	0	34	theme	γ-Glutamic	29:38	arg1	acid					40:43	γ-Glutamic acid	29:43	γ-Glutamic acid	29:43	Optimized Production of Poly(γ-Glutamic acid) By Bacillus sp.					
31280522	6	35	theme	significant	816:826	arg1	components					835:844	significant medium components	816:844	significant medium components	816:844	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	7	36	theme	optimized	964:972	arg1	medium					974:979	The optimized medium	960:979	The optimized medium	960:979	The optimized medium was validated experimentally, and γ-PGA production increased significantly from 3.59 g/l (0.33 g/l/h) to 44.04 g/l (3.67 g/l/h) when strain FBL-2 was cultivated under the optimal medium developed by the statistical approach, as compared to non-optimized medium.					
31280522	6	37	theme	optimum	798:804	arg1	components					835:844	significant medium components	816:844	significant medium components	816:844	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	37	theme	optimum	798:804	arg1	g/l					875:877	sucrose 51.73 g/l	861:877	sucrose 51.73 g/l	861:877	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	37	theme	optimum	798:804	arg1	amount					806:811	The optimum amount	794:811	The optimum amount of significant medium components	794:844	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	7	38	theme	statistical	1184:1194	arg1	approach					1196:1203	the statistical approach	1180:1203	the statistical approach	1180:1203	The optimized medium was validated experimentally, and γ-PGA production increased significantly from 3.59 g/l (0.33 g/l/h) to 44.04 g/l (3.67 g/l/h) when strain FBL-2 was cultivated under the optimal medium developed by the statistical approach, as compared to non-optimized medium.					
31280522	5	39	theme	γ-PGA	776:780	arg1	production					782:791	γ-PGA production	776:791	γ-PGA production	776:791	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	4	40	theme	γ-PGA	487:491	arg1	yield					478:482	the yield	474:482	the yield of γ-PGA	474:491	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	4	41	theme	sources	378:384	arg1	effect					340:345	the effect	336:345	the effect of carbon sources and nitrogen sources on γ-PGA production	336:404	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	5	42	theme	production	782:791	arg1	improvement					761:771	improvement	761:771	improvement of γ-PGA production	761:791	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	43	dep	identify	641:648	arg1	acid					751:754	citric acid	744:754	citric acid	744:754	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	43	dep	identify	641:648	arg1	sucrose					699:705	sucrose	699:705	sucrose	699:705	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	43	dep	identify	641:648	arg1	acid					719:722	L-glutamic acid	708:722	L-glutamic acid	708:722	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	43	dep	identify	641:648	arg1	extract					731:737	yeast extract	725:737	yeast extract	725:737	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	2	44	theme	present	144:150	arg1	study					152:156	the present study	140:156	the present study	140:156	In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp.					
31280522	5	45	theme	effective	512:520	arg1	nutrients					522:530	effective nutrients	512:530	effective nutrients	512:530	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	7	46	theme	strain	1114:1119	arg1	FBL-2					1121:1125	strain FBL-2	1114:1125	strain FBL-2	1114:1125	The optimized medium was validated experimentally, and γ-PGA production increased significantly from 3.59 g/l (0.33 g/l/h) to 44.04 g/l (3.67 g/l/h) when strain FBL-2 was cultivated under the optimal medium developed by the statistical approach, as compared to non-optimized medium.					
31280522	2	47	theme	γ-glutamic	184:193	arg1	poly					179:182	poly	179:182	poly(γ-glutamic acid) (γ-PGA) production	179:218	In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp.					
31280522	2	47	theme	γ-glutamic	184:193	arg1	acid					195:198	γ-glutamic acid	184:198	γ-glutamic acid	184:198	In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp.					
31280522	6	48	theme	citric	937:942	arg1	g/l					875:877	sucrose 51.73 g/l	861:877	sucrose 51.73 g/l	861:877	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	48	theme	citric	937:942	arg1	acid					944:947	citric acid	937:947	citric acid 10.04 g/l	937:957	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	49	theme	L-glutamic	880:889	arg1	g/l					928:930	yeast extract 13.25 g/l	908:930	yeast extract 13.25 g/l	908:930	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	49	theme	L-glutamic	880:889	arg1	g/l					875:877	sucrose 51.73 g/l	861:877	sucrose 51.73 g/l	861:877	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	49	theme	L-glutamic	880:889	arg1	acid					891:894	L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l,	880:931	acid	891:894	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	2	50	theme	poly	179:182	arg1	production					209:218	poly(γ-glutamic acid) (γ-PGA) production	179:218	poly(γ-glutamic acid) (γ-PGA) production	179:218	In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp.					
31280522	5	51	theme	composite	575:583	arg1	CCD					593:595	CCD	593:595	CCD	593:595	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	5	51	theme	composite	575:583	arg1	design					585:590	central composite design	567:590	central composite design (CCD)	567:596	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	1	52	theme	Central	111:117	arg1	Design					129:134	Central Composite Design	111:134	Central Composite Design	111:134	FBL-2 through Response Surface Methodology Using Central Composite Design.					
31280522	2	53	theme	production	209:218	arg1	optimization					163:174	the optimization	159:174	the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp	159:233	In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp.					
31280522	1	54	theme	Composite	119:127	arg1	Design					129:134	Central Composite Design	111:134	Central Composite Design	111:134	FBL-2 through Response Surface Methodology Using Central Composite Design.					
31280522	2	55	dep	optimization	163:174	arg1	study					152:156	the present study	140:156	the present study	140:156	In the present study, the optimization of poly(γ-glutamic acid) (γ-PGA) production by Bacillus sp.					
31280522	4	56	used	utilized	414:421	arg2	method					305:310	One-factor-at-a-time method	284:310	One-factor-at-a-time method	284:310	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	4	57	theme	γ-PGA	389:393	arg1	production					395:404	γ-PGA production	389:404	γ-PGA production	389:404	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
31280522	5	58	theme	L-glutamic	708:717	arg1	acid					719:722	L-glutamic acid	708:722	L-glutamic acid	708:722	After identifying effective nutrients, response surface methodology with central composite design (CCD) was used to obtain a mathematical model to identify the optimum concentrations of the key nutrients (sucrose, L-glutamic acid, yeast extract, and citric acid) for improvement of γ-PGA production.					
31280522	6	59	theme	sucrose	861:867	arg1	components					835:844	significant medium components	816:844	significant medium components	816:844	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	59	theme	sucrose	861:867	arg1	acid					944:947	citric acid	937:947	citric acid 10.04 g/l	937:957	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	59	theme	sucrose	861:867	arg1	g/l					875:877	sucrose 51.73 g/l	861:877	sucrose 51.73 g/l	861:877	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	59	theme	sucrose	861:867	arg1	acid					891:894	L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l,	880:931	acid	891:894	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	6	59	theme	sucrose	861:867	arg1	amount					806:811	The optimum amount	794:811	The optimum amount of significant medium components	794:844	The optimum amount of significant medium components appeared to be sucrose 51.73 g/l, L-glutamic acid 105.30 g/l, yeast extract 13.25 g/l, and citric acid 10.04 g/l.					
31280522	4	60	theme	One-factor-at-a-time	284:303	arg1	method					305:310	One-factor-at-a-time method	284:310	One-factor-at-a-time method	284:310	One-factor-at-a-time method was used to investigate the effect of carbon sources and nitrogen sources on γ-PGA production and was utilized to select the most significant nutrients affecting the yield of γ-PGA.					
30664246	4	0	theme	DBD	866:868	arg1	treatment					877:885	DBD plasma treatment	866:885	DBD plasma treatment	866:885	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	6	1	theme	thermal	1281:1287	arg1	properties					1289:1298	the mechanical, barrier, and thermal properties	1252:1298	the mechanical, barrier, and thermal properties of WPC/WCS films	1252:1315	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.					
30664246	2	2	theme	protein	368:374	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	4	3	theme	X-ray	836:840	arg1	diffraction					842:852	X-ray diffraction	836:852	X-ray diffraction	836:852	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	7	4	theme	film	1422:1425	arg1	properties					1427:1436	edible film properties	1415:1436	edible film properties	1415:1436	The DBD plasma can be potentially applied in the enhancement of edible film properties.					
30664246	3	5	theme	treatment	543:551	arg1	parameters					553:562	the proper plasma treatment parameters	525:562	the proper plasma treatment parameters (400 W to 60 s)	525:578	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	3	5	theme	treatment	543:551	arg1	s					577:577	400 W to 60 s	565:577	400 W to 60 s	565:577	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	6	6	theme	films	1311:1315	arg1	properties					1289:1298	the mechanical, barrier, and thermal properties	1252:1298	the mechanical, barrier, and thermal properties of WPC/WCS films	1252:1315	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.					
30664246	4	7	theme	oxygen-containing	917:933	arg1	groups					935:940	oxygen-containing groups	917:940	oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure	917:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	0	8	theme	Composite	148:156	arg1	Film					158:161	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	5	9	theme	plasma	1065:1070	arg1	technology					1082:1091	proper DBD plasma treatment technology	1054:1091	proper DBD plasma treatment technology	1054:1091	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	2	10	theme	film	306:309	arg1	structure					319:327	film surface structure	306:327	film surface structure	306:327	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	5	11	theme	technology	1082:1091	arg1	use					1047:1049	use	1047:1049	use of proper DBD plasma treatment technology	1047:1091	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	1	12	theme	polymer	223:229	arg1	technology					252:261	polymer surface modification technology	223:261	polymer surface modification technology	223:261	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	6	13	dep	APPLICATION	1206:1216	arg1	improve					1244:1250	improve	1244:1250	can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution	1240:1348	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.					
30664246	3	14	theme	contact	742:748	arg1	angle					750:754	the water contact angle	732:754	the water contact angle	732:754	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	4	15	dep	damaging	982:989	arg1	structure					1012:1020	the internal crystal structure	991:1020	damaging the internal crystal structure	982:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	4	16	from	content	906:912	arg1	surfaces					962:969	the WPC/WCS film surfaces	945:969	the WPC/WCS film surfaces instead of damaging the internal crystal structure	945:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	6	17	theme	DBD	1219:1221	arg1	treatment					1230:1238	DBD plasma treatment	1219:1238	DBD plasma treatment	1219:1238	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.					
30664246	5	18	theme	proper	1054:1059	arg1	technology					1082:1091	proper DBD plasma treatment technology	1054:1091	proper DBD plasma treatment technology	1054:1091	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	3	19	theme	tensile	616:622	arg1	strength					624:631	tensile strength	616:631	tensile strength	616:631	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	1	20	theme	Dielectric	164:173	arg1	discharge					183:191	Dielectric barrier discharge	164:191	Dielectric barrier discharge (DBD) plasma	164:204	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	1	20	theme	Dielectric	164:173	arg1	DBD					194:196	DBD	194:196	DBD	194:196	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	0	21	theme	Barrier	64:70	arg1	Concentrate/Wheat					110:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat	53:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	1	22	theme	discharge	183:191	arg1	type					215:218	a new type	209:218	a new type of polymer surface modification technology	209:261	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	1	22	theme	discharge	183:191	arg1	plasma					199:204	Dielectric barrier discharge (DBD) plasma	164:204	Dielectric barrier discharge (DBD) plasma	164:204	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	0	23	theme	Plasma-Treated	82:95	arg1	Concentrate/Wheat					110:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat	53:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	4	24	theme	WPC/WCS	949:955	arg1	surfaces					962:969	the WPC/WCS film surfaces	945:969	the WPC/WCS film surfaces instead of damaging the internal crystal structure	945:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	0	25	from	Effects	0:6	arg1	Surface					11:17	Surface	11:17	Surface	11:17	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	0	25	from	Effects	0:6	arg1	Properties					39:48	Physicochemical Properties	23:48	Physicochemical Properties	23:48	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	0	26	theme	Protein	102:108	arg1	Concentrate/Wheat					110:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat	53:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	3	27	from	break	722:726	arg1	angle					750:754	the water contact angle	732:754	the water contact angle	732:754	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	3	27	from	break	722:726	arg1	elongation					708:717	elongation	708:717	elongation at break	708:726	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	4	28	theme	X-ray	757:761	arg1	spectroscopy					777:788	X-ray photoelectron spectroscopy	757:788	X-ray photoelectron spectroscopy	757:788	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	2	29	theme	starch	413:418	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	0	30	theme	Cross-Linked	128:139	arg1	Film					158:161	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	2	31	theme	plasma	484:489	arg1	parameters					491:500	different plasma parameters	474:500	different plasma parameters	474:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	5	32	contain	has	1093:1095	arg1	use					1047:1049	use	1047:1049	use of proper DBD plasma treatment technology	1047:1091	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	5	32	contain	has	1093:1095	arg2	effect					1108:1113	a positive effect	1097:1113	a positive effect	1097:1113	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	3	33	theme	proper	529:534	arg1	parameters					553:562	the proper plasma treatment parameters	525:562	the proper plasma treatment parameters (400 W to 60 s)	525:578	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	3	33	theme	proper	529:534	arg1	s					577:577	400 W to 60 s	565:577	400 W to 60 s	565:577	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	2	34	theme	/wheat	393:398	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	2	35	theme	composite	426:434	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	5	36	theme	films	1189:1193	arg1	properties					1145:1154	mechanical and barrier properties	1122:1154	properties	1145:1154	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	5	36	theme	films	1189:1193	arg1	stability					1168:1176	thermal stability	1160:1176	thermal stability	1160:1176	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	4	37	theme	crystal	1004:1010	arg1	structure					1012:1020	the internal crystal structure	991:1020	damaging the internal crystal structure	982:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	2	38	theme	WCS	421:423	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	0	39	theme	Physicochemical	23:37	arg1	Properties					39:48	Physicochemical Properties	23:48	Physicochemical Properties	23:48	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	2	40	theme	different	474:482	arg1	parameters					491:500	different plasma parameters	474:500	different plasma parameters	474:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	2	41	link	cross-linked	400:411	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	2	42	theme	whey	363:366	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	5	43	theme	thermal	1160:1166	arg1	stability					1168:1176	thermal stability	1160:1176	thermal stability	1160:1176	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	7	44	theme	DBD	1355:1357	arg1	plasma					1359:1364	The DBD plasma	1351:1364	The DBD plasma	1351:1364	The DBD plasma can be potentially applied in the enhancement of edible film properties.					
30664246	1	45	theme	surface	231:237	arg1	technology					252:261	polymer surface modification technology	223:261	polymer surface modification technology	223:261	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	8	46	theme	WPC/WCS	1439:1445	arg1	films					1447:1451	WPC/WCS films	1439:1451	WPC/WCS films	1439:1451	WPC/WCS films are more environmentally friendly than plastics and can be a replacement for traditional plastics.					
30664246	8	46	theme	WPC/WCS	1439:1445	arg1	friendly					1478:1485	more environmentally friendly	1457:1485	more environmentally friendly than plastics	1457:1499	WPC/WCS films are more environmentally friendly than plastics and can be a replacement for traditional plastics.					
30664246	2	47	theme	WPC	389:391	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	1	48	theme	technology	252:261	arg1	type					215:218	a new type	209:218	a new type of polymer surface modification technology	209:261	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	1	48	theme	technology	252:261	arg1	plasma					199:204	Dielectric barrier discharge (DBD) plasma	164:204	Dielectric barrier discharge (DBD) plasma	164:204	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	3	49	theme	film	690:693	arg1	roughness					605:613	surface roughness	597:613	surface roughness	597:613	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	3	49	theme	film	690:693	arg1	strength					624:631	tensile strength	616:631	tensile strength	616:631	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	3	49	theme	film	690:693	arg1	properties					642:651	barrier properties	634:651	barrier properties	634:651	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	3	49	theme	film	690:693	arg1	stability					666:674	thermal stability	658:674	thermal stability	658:674	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	2	50	theme	concentrate	376:386	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	4	51	from	surfaces	962:969	arg1	content					906:912	the content	902:912	the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure	902:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	4	52	theme	plasma	870:875	arg1	treatment					877:885	DBD plasma treatment	866:885	DBD plasma treatment	866:885	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	6	53	theme	barrier	1268:1274	arg1	properties					1289:1298	the mechanical, barrier, and thermal properties	1252:1298	the mechanical, barrier, and thermal properties of WPC/WCS films	1252:1315	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.					
30664246	3	54	theme	plasma	536:541	arg1	parameters					553:562	the proper plasma treatment parameters	525:562	the proper plasma treatment parameters (400 W to 60 s)	525:578	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	3	54	theme	plasma	536:541	arg1	s					577:577	400 W to 60 s	565:577	400 W to 60 s	565:577	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	4	55	dep	Fourier	791:797	arg1	transform					799:807	transform	799:807	transform infrared spectroscopy	799:829	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	7	56	theme	properties	1427:1436	arg1	enhancement					1400:1410	the enhancement	1396:1410	the enhancement of edible film properties	1396:1436	The DBD plasma can be potentially applied in the enhancement of edible film properties.					
30664246	6	57	theme	mechanical	1256:1265	arg1	properties					1289:1298	the mechanical, barrier, and thermal properties	1252:1298	the mechanical, barrier, and thermal properties of WPC/WCS films	1252:1315	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.					
30664246	7	58	theme	edible	1415:1420	arg1	properties					1427:1436	edible film properties	1415:1436	edible film properties	1415:1436	The DBD plasma can be potentially applied in the enhancement of edible film properties.					
30664246	3	59	theme	water	736:740	arg1	angle					750:754	the water contact angle	732:754	the water contact angle	732:754	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	0	60	theme	Film	158:161	arg1	Surface					11:17	Surface	11:17	Surface	11:17	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	0	60	theme	Film	158:161	arg1	Properties					39:48	Physicochemical Properties	23:48	Physicochemical Properties	23:48	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	2	61	theme	surface	311:317	arg1	structure					319:327	film surface structure	306:327	film surface structure	306:327	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	1	62	theme	new	211:213	arg1	type					215:218	a new type	209:218	a new type of polymer surface modification technology	209:261	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	1	62	theme	new	211:213	arg1	plasma					199:204	Dielectric barrier discharge (DBD) plasma	164:204	Dielectric barrier discharge (DBD) plasma	164:204	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	5	63	theme	treatment	1072:1080	arg1	technology					1082:1091	proper DBD plasma treatment technology	1054:1091	proper DBD plasma treatment technology	1054:1091	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	4	64	theme	groups	935:940	arg1	content					906:912	the content	902:912	the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure	902:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	8	65	theme	environmentally	1462:1476	arg1	films					1447:1451	WPC/WCS films	1439:1451	WPC/WCS films	1439:1451	WPC/WCS films are more environmentally friendly than plastics and can be a replacement for traditional plastics.					
30664246	8	65	theme	environmentally	1462:1476	arg1	friendly					1478:1485	more environmentally friendly	1457:1485	more environmentally friendly than plastics	1457:1499	WPC/WCS films are more environmentally friendly than plastics and can be a replacement for traditional plastics.					
30664246	6	66	theme	plasma	1223:1228	arg1	treatment					1230:1238	DBD plasma treatment	1219:1238	DBD plasma treatment	1219:1238	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.					
30664246	6	67	theme	WPC/WCS	1303:1309	arg1	films					1311:1315	WPC/WCS films	1303:1315	WPC/WCS films	1303:1315	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.					
30664246	0	68	theme	Discharge	72:80	arg1	Concentrate/Wheat					110:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat	53:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	5	69	theme	DBD	1061:1063	arg1	technology					1082:1091	proper DBD plasma treatment technology	1054:1091	proper DBD plasma treatment technology	1054:1091	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	3	70	theme	surface	597:603	arg1	roughness					605:613	surface roughness	597:613	surface roughness	597:613	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	2	71	with	treatment	459:467	arg1	parameters					491:500	different plasma parameters	474:500	different plasma parameters	474:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	1	72	theme	barrier	175:181	arg1	discharge					183:191	Dielectric barrier discharge	164:191	Dielectric barrier discharge (DBD) plasma	164:204	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	1	72	theme	barrier	175:181	arg1	DBD					194:196	DBD	194:196	DBD	194:196	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	2	73	from	changes	295:301	arg1	structure					319:327	film surface structure	306:327	film surface structure	306:327	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	2	73	from	changes	295:301	arg1	properties					349:358	physicochemical properties	333:358	physicochemical properties	333:358	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	6	74	theme	PRACTICAL	1196:1204	arg1	APPLICATION					1206:1216	PRACTICAL APPLICATION	1196:1216	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.	1196:1349	PRACTICAL APPLICATION: DBD plasma treatment can improve the mechanical, barrier, and thermal properties of WPC/WCS films without generating any pollution.					
30664246	0	75	theme	Whey	97:100	arg1	Concentrate/Wheat					110:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat	53:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	5	76	theme	mechanical	1122:1131	arg1	properties					1145:1154	mechanical and barrier properties	1122:1154	properties	1145:1154	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	3	77	dep	60	574:575	arg1	to					571:572	to	571:572	to	571:572	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	4	78	theme	damaging	982:989	arg1	surfaces					962:969	the WPC/WCS film surfaces	945:969	the WPC/WCS film surfaces instead of damaging the internal crystal structure	945:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	0	79	theme	Concentrate/Wheat	110:126	arg1	Film					158:161	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	5	80	theme	barrier	1137:1143	arg1	properties					1145:1154	mechanical and barrier properties	1122:1154	properties	1145:1154	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	8	81	theme	traditional	1530:1540	arg1	plastics					1542:1549	traditional plastics	1530:1549	traditional plastics	1530:1549	WPC/WCS films are more environmentally friendly than plastics and can be a replacement for traditional plastics.					
30664246	0	82	theme	Starch	141:146	arg1	Film					158:161	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	5	83	theme	positive	1099:1106	arg1	effect					1108:1113	a positive effect	1097:1113	a positive effect	1097:1113	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	4	84	theme	film	957:960	arg1	surfaces					962:969	the WPC/WCS film surfaces	945:969	the WPC/WCS film surfaces instead of damaging the internal crystal structure	945:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	5	85	dep	properties	1145:1154	arg1	the					1118:1120	the	1118:1120	the	1118:1120	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	2	86	theme	cross-linked	400:411	arg1	films					436:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films	363:440	whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters	363:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	2	87	theme	DBD	448:450	arg1	treatment					459:467	DBD plasma treatment	448:467	DBD plasma treatment with different plasma parameters	448:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	4	88	from	groups	935:940	arg1	surfaces					962:969	the WPC/WCS film surfaces	945:969	the WPC/WCS film surfaces instead of damaging the internal crystal structure	945:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	5	89	theme	WPC/WCS	1181:1187	arg1	films					1189:1193	WPC/WCS films	1181:1193	WPC/WCS films	1181:1193	The results showed that use of proper DBD plasma treatment technology has a positive effect on the mechanical and barrier properties and thermal stability of WPC/WCS films.					
30664246	2	90	theme	films	436:440	arg1	structure					319:327	film surface structure	306:327	film surface structure	306:327	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	2	90	theme	films	436:440	arg1	properties					349:358	physicochemical properties	333:358	physicochemical properties	333:358	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	3	91	theme	barrier	634:640	arg1	properties					642:651	barrier properties	634:651	barrier properties	634:651	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	4	92	theme	internal	995:1002	arg1	structure					1012:1020	the internal crystal structure	991:1020	damaging the internal crystal structure	982:1020	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	0	93	theme	Dielectric	53:62	arg1	Concentrate/Wheat					110:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat	53:126	Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film	53:161	Effects on Surface and Physicochemical Properties of Dielectric Barrier Discharge Plasma-Treated Whey Protein Concentrate/Wheat Cross-Linked Starch Composite Film.					
30664246	3	94	theme	thermal	658:664	arg1	stability					666:674	thermal stability	658:674	thermal stability	658:674	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	4	95	theme	photoelectron	763:775	arg1	spectroscopy					777:788	X-ray photoelectron spectroscopy	757:788	X-ray photoelectron spectroscopy	757:788	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	2	96	theme	physicochemical	333:347	arg1	properties					349:358	physicochemical properties	333:358	physicochemical properties	333:358	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
30664246	1	97	theme	modification	239:250	arg1	technology					252:261	polymer surface modification technology	223:261	polymer surface modification technology	223:261	Dielectric barrier discharge (DBD) plasma is a new type of polymer surface modification technology.					
30664246	4	98	dep	transform	799:807	arg1	infrared					809:816	infrared	809:816	transform infrared spectroscopy	799:829	X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction showed that DBD plasma treatment could increase the content of oxygen-containing groups on the WPC/WCS film surfaces instead of damaging the internal crystal structure.					
30664246	3	99	theme	edible	683:688	arg1	film					690:693	the edible film	679:693	the edible film	679:693	The results show that the proper plasma treatment parameters (400 W to 60 s) can increase the surface roughness, tensile strength, barrier properties, and thermal stability of the edible film and decrease elongation at break and the water contact angle.					
30664246	2	100	theme	plasma	452:457	arg1	treatment					459:467	DBD plasma treatment	448:467	DBD plasma treatment with different plasma parameters	448:500	This study is mainly about the changes in film surface structure and physicochemical properties of whey protein concentrate (WPC)/wheat cross-linked starch (WCS) composite films after DBD plasma treatment with different plasma parameters.					
31849889	8	0	located	found	1324:1328	arg2	capable					1384:1390	capable	1384:1390	capable	1384:1390	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	0	located	found	1324:1328	arg2	core					1319:1322	the N-linked mannan core	1299:1322	the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant	1299:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	0	located	found	1324:1328	arg1	surface					1337:1343	the surface	1333:1343	the surface of C. tropicalis och1Δ null mutant	1333:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	9	1	theme	mouse	1630:1634	arg1	model					1636:1640	the mouse model	1626:1640	the mouse model	1626:1640	Both mutant strains showed virulence attenuation in the Galleria mellonella and the mouse model of systemic candidiasis.					
31849889	2	2	theme	mannan	265:270	arg1	chain					278:282	the N-linked mannan outer chain	252:282	the N-linked mannan outer chain	252:282	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	1	3	theme	wall	111:114	arg1	Mannans					74:80	Mannans	74:80	Mannans	74:80	Mannans are components of the fungal wall attached to proteins via N- or O-linkages.					
31849889	1	3	theme	wall	111:114	arg1	components					86:95	components	86:95	components of the fungal wall attached to proteins via N- or O-linkages	86:156	Mannans are components of the fungal wall attached to proteins via N- or O-linkages.					
31849889	3	4	theme	O-linked	479:486	arg1	mannans					488:494	O-linked mannans	479:494	O-linked mannans	479:494	This cation is the cofactor of Golgi-resident mannosyltransferases, and thus Pmr1 is involved in the synthesis of both N- and O-linked mannans.					
31849889	5	5	theme	mannan	813:818	arg1	content					820:826	the wall mannan content	804:826	the wall mannan content	804:826	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	9	6	theme	candidiasis	1654:1664	arg1	model					1636:1640	the mouse model	1626:1640	the mouse model	1626:1640	Both mutant strains showed virulence attenuation in the Galleria mellonella and the mouse model of systemic candidiasis.					
31849889	9	6	theme	candidiasis	1654:1664	arg1	mellonella					1611:1620	the Galleria mellonella	1598:1620	the Galleria mellonella	1598:1620	Both mutant strains showed virulence attenuation in the Galleria mellonella and the mouse model of systemic candidiasis.					
31849889	2	7	link	N-linked	256:263	arg1	chain					278:282	the N-linked mannan outer chain	252:282	the N-linked mannan outer chain	252:282	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	8	8	from	interaction	1533:1543	arg1	present					1487:1493	present	1487:1493	present	1487:1493	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	8	from	interaction	1533:1543	arg1	pathway					1455:1461	a mannan-independent pathway	1434:1461	a mannan-independent pathway for IL-10 production	1434:1482	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	4	9	contain	have	516:519	arg1	we					503:504	we	503:504	we	503:504	Since we currently have limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery, we disrupted OCH1 and PMR1 in this organism.					
31849889	4	9	contain	have	516:519	arg2	information					529:539	limited information	521:539	limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery	521:627	Since we currently have limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery, we disrupted OCH1 and PMR1 in this organism.					
31849889	4	10	theme	tropicalis	586:595	arg1	machinery					619:627	the Candida tropicalis protein mannosylation machinery	574:627	the Candida tropicalis protein mannosylation machinery	574:627	Since we currently have limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery, we disrupted OCH1 and PMR1 in this organism.					
31849889	8	11	attach	present	1487:1493	arg2	present					1487:1493	present	1487:1493	present	1487:1493	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	11	attach	present	1487:1493	arg1	interaction					1533:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	11	attach	present	1487:1493	arg2	pathway					1455:1461	a mannan-independent pathway	1434:1461	a mannan-independent pathway for IL-10 production	1434:1482	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	4	12	theme	mannosylation	605:617	arg1	machinery					619:627	the Candida tropicalis protein mannosylation machinery	574:627	the Candida tropicalis protein mannosylation machinery	574:627	Since we currently have limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery, we disrupted OCH1 and PMR1 in this organism.					
31849889	5	13	theme	cellular	770:777	arg1	times					743:747	increased doubling times	724:747	increased doubling times	724:747	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	13	theme	cellular	770:777	arg1	morphology					779:788	cellular morphology	770:788	cellular morphology	770:788	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	8	14	theme	cytokine	1404:1411	arg1	production					1413:1422	cytokine production	1404:1422	cytokine production	1404:1422	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	10	15	theme	tropicalis-host	1758:1772	arg1	interaction					1774:1784	the C. tropicalis-host interaction	1751:1784	the C. tropicalis-host interaction	1751:1784	Therefore, mannans are relevant for cell wall composition and organization, and for the C. tropicalis-host interaction.					
31849889	5	16	theme	perturbing	866:875	arg1	agents					877:882	wall perturbing agents	861:882	wall perturbing agents	861:882	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	7	17	theme	human	1097:1101	arg1	cells					1115:1119	human mononuclear cells	1097:1119	human mononuclear cells	1097:1119	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	2	18	theme	outer	272:276	arg1	chain					278:282	the N-linked mannan outer chain	252:282	the N-linked mannan outer chain	252:282	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	6	19	theme	wall	976:979	arg1	surface					981:987	the wall surface	972:987	the wall surface of both mutant strains	972:1010	These changes were accompanied by increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains.					
31849889	7	20	theme	cytokine	1186:1193	arg1	production					1195:1204	cytokine production	1186:1204	cytokine production	1186:1204	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	2	21	theme	first	230:234	arg1	unit					244:247	the first mannose unit	226:247	the first mannose unit to the N-linked mannan outer chain	226:282	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	5	22	theme	increased	833:841	arg1	susceptibility					843:856	increased susceptibility	833:856	increased susceptibility to wall perturbing agents	833:882	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	8	23	theme	mannan-independent	1436:1453	arg1	present					1487:1493	present	1487:1493	present	1487:1493	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	23	theme	mannan-independent	1436:1453	arg1	pathway					1455:1461	a mannan-independent pathway	1434:1461	a mannan-independent pathway for IL-10 production	1434:1482	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	5	24	theme	increased	724:732	arg1	times					743:747	increased doubling times	724:747	increased doubling times	724:747	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	24	theme	increased	724:732	arg1	colony					759:764	aberrant colony	750:764	aberrant colony	750:764	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	24	theme	increased	724:732	arg1	morphology					779:788	cellular morphology	770:788	cellular morphology	770:788	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	25	from	susceptibility	843:856	arg1	content					820:826	the wall mannan content	804:826	the wall mannan content	804:826	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	0	26	from	Role	0:3	arg1	Interaction					61:71	the Candida tropicalis-Host Interaction	33:71	the Candida tropicalis-Host Interaction	33:71	Role of Protein Mannosylation in the Candida tropicalis-Host Interaction.					
31849889	6	27	from	surface	981:987	arg1	exposure					929:936	increased exposure	919:936	increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains	919:1010	These changes were accompanied by increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains.					
31849889	8	28	theme	N-linked	1303:1310	arg1	core					1319:1322	the N-linked mannan core	1299:1322	the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant	1299:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	28	theme	N-linked	1303:1310	arg1	capable					1384:1390	capable	1384:1390	capable	1384:1390	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	9	29	theme	mutant	1551:1556	arg1	strains					1558:1564	Both mutant strains	1546:1564	Both mutant strains	1546:1564	Both mutant strains showed virulence attenuation in the Galleria mellonella and the mouse model of systemic candidiasis.					
31849889	7	30	theme	key	1163:1165	arg1	β1,3-glucan					1147:1157	β1,3-glucan	1147:1157	β1,3-glucan	1147:1157	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	7	30	theme	key	1163:1165	arg1	ligands					1167:1173	key ligands	1163:1173	key ligands	1163:1173	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	7	30	theme	key	1163:1165	arg1	mannans					1135:1141	N-linked mannans	1126:1141	N-linked mannans	1126:1141	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	3	31	theme	Golgi-resident	384:397	arg1	mannosyltransferases					399:418	Golgi-resident mannosyltransferases	384:418	Golgi-resident mannosyltransferases	384:418	This cation is the cofactor of Golgi-resident mannosyltransferases, and thus Pmr1 is involved in the synthesis of both N- and O-linked mannans.					
31849889	5	32	theme	doubling	734:741	arg1	times					743:747	increased doubling times	724:747	increased doubling times	724:747	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	32	theme	doubling	734:741	arg1	colony					759:764	aberrant colony	750:764	aberrant colony	750:764	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	32	theme	doubling	734:741	arg1	morphology					779:788	cellular morphology	770:788	cellular morphology	770:788	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	2	33	theme	Golgi	340:344	arg1	lumen					346:350	the Golgi lumen	336:350	the Golgi lumen	336:350	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	2	34	theme	mannose	236:242	arg1	unit					244:247	the first mannose unit	226:247	the first mannose unit to the N-linked mannan outer chain	226:282	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	0	35	theme	Protein	8:14	arg1	Mannosylation					16:28	Protein Mannosylation	8:28	Protein Mannosylation	8:28	Role of Protein Mannosylation in the Candida tropicalis-Host Interaction.					
31849889	1	36	theme	fungal	104:109	arg1	wall					111:114	the fungal wall	100:114	the fungal wall attached to proteins via N- or O-linkages	100:156	Mannans are components of the fungal wall attached to proteins via N- or O-linkages.					
31849889	8	37	located	present	1487:1493	arg2	present					1487:1493	present	1487:1493	present	1487:1493	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	37	located	present	1487:1493	arg2	pathway					1455:1461	a mannan-independent pathway	1434:1461	a mannan-independent pathway for IL-10 production	1434:1482	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	37	located	present	1487:1493	arg1	interaction					1533:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	6	38	theme	mutant	997:1002	arg1	strains					1004:1010	both mutant strains	992:1010	both mutant strains	992:1010	These changes were accompanied by increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains.					
31849889	8	39	theme	tropicalis-mononuclear	1505:1526	arg1	interaction					1533:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	2	40	theme	Candida	162:168	arg1	albicans					170:177	Candida albicans	162:177	Candida albicans	162:177	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	8	41	theme	C.	1348:1349	arg1	tropicalis					1351:1360	C. tropicalis	1348:1360	C. tropicalis och1Δ null mutant	1348:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	42	from	present	1487:1493	arg1	interaction					1533:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	43	theme	och1Δ	1362:1366	arg1	mutant					1373:1378	C. tropicalis och1Δ null mutant	1348:1378	C. tropicalis och1Δ null mutant	1348:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	5	44	theme	aberrant	750:757	arg1	times					743:747	increased doubling times	724:747	increased doubling times	724:747	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	44	theme	aberrant	750:757	arg1	colony					759:764	aberrant colony	750:764	aberrant colony	750:764	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	8	45	theme	mutant	1373:1378	arg1	surface					1337:1343	the surface	1333:1343	the surface of C. tropicalis och1Δ null mutant	1333:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	4	46	theme	genetic	551:557	arg1	network					559:565	the genetic network	547:565	the genetic network behind the Candida tropicalis protein mannosylation machinery	547:627	Since we currently have limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery, we disrupted OCH1 and PMR1 in this organism.					
31849889	10	47	theme	wall	1708:1711	arg1	composition					1713:1723	cell wall composition	1703:1723	cell wall composition	1703:1723	Therefore, mannans are relevant for cell wall composition and organization, and for the C. tropicalis-host interaction.					
31849889	7	48	theme	co-stimulatory	1211:1224	arg1	pathway					1226:1232	a co-stimulatory pathway	1209:1232	a co-stimulatory pathway involving dectin-1 and mannose receptor	1209:1272	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	7	49	theme	cytokine	1074:1081	arg1	production					1083:1092	cytokine production	1074:1092	cytokine production by human mononuclear cells	1074:1119	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	4	50	theme	limited	521:527	arg1	information					529:539	limited information	521:539	limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery	521:627	Since we currently have limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery, we disrupted OCH1 and PMR1 in this organism.					
31849889	3	51	theme	mannans	488:494	arg1	synthesis					454:462	the synthesis	450:462	the synthesis of both N- and O-linked mannans	450:494	This cation is the cofactor of Golgi-resident mannosyltransferases, and thus Pmr1 is involved in the synthesis of both N- and O-linked mannans.					
31849889	2	52	theme	N-linked	256:263	arg1	chain					278:282	the N-linked mannan outer chain	252:282	the N-linked mannan outer chain	252:282	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	5	53	theme	wall	808:811	arg1	content					820:826	the wall mannan content	804:826	the wall mannan content	804:826	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	0	54	theme	Candida	37:43	arg1	Interaction					61:71	the Candida tropicalis-Host Interaction	33:71	the Candida tropicalis-Host Interaction	33:71	Role of Protein Mannosylation in the Candida tropicalis-Host Interaction.					
31849889	9	55	theme	systemic	1645:1652	arg1	candidiasis					1654:1664	systemic candidiasis	1645:1664	systemic candidiasis	1645:1664	Both mutant strains showed virulence attenuation in the Galleria mellonella and the mouse model of systemic candidiasis.					
31849889	10	56	theme	C.	1755:1756	arg1	interaction					1774:1784	the C. tropicalis-host interaction	1751:1784	the C. tropicalis-host interaction	1751:1784	Therefore, mannans are relevant for cell wall composition and organization, and for the C. tropicalis-host interaction.					
31849889	7	57	theme	O-linked	1037:1044	arg1	mannans					1046:1052	O-linked mannans	1037:1052	O-linked mannans	1037:1052	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	8	58	link	N-linked	1303:1310	arg1	core					1319:1322	the N-linked mannan core	1299:1322	the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant	1299:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	58	link	N-linked	1303:1310	arg1	capable					1384:1390	capable	1384:1390	capable	1384:1390	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	4	59	theme	Candida	578:584	arg1	tropicalis					586:595	Candida tropicalis	578:595	the Candida tropicalis protein mannosylation machinery	574:627	Since we currently have limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery, we disrupted OCH1 and PMR1 in this organism.					
31849889	4	60	theme	protein	597:603	arg1	machinery					619:627	the Candida tropicalis protein mannosylation machinery	574:627	the Candida tropicalis protein mannosylation machinery	574:627	Since we currently have limited information about the genetic network behind the Candida tropicalis protein mannosylation machinery, we disrupted OCH1 and PMR1 in this organism.					
31849889	5	61	theme	wall	861:864	arg1	agents					877:882	wall perturbing agents	861:882	wall perturbing agents	861:882	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	62	from	reduction	791:799	arg1	content					820:826	the wall mannan content	804:826	the wall mannan content	804:826	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	63	theme	och1Δ	703:707	arg1	mutants					709:715	The C. tropicalis pmr1Δ and och1Δ mutants	675:715	mutants	709:715	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	7	64	link	N-linked	1126:1133	arg1	β1,3-glucan					1147:1157	β1,3-glucan	1147:1157	β1,3-glucan	1147:1157	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	7	64	link	N-linked	1126:1133	arg1	ligands					1167:1173	key ligands	1163:1173	key ligands	1163:1173	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	7	64	link	N-linked	1126:1133	arg1	mannans					1135:1141	N-linked mannans	1126:1141	N-linked mannans	1126:1141	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	5	65	theme	tropicalis	682:691	arg1	pmr1Δ					693:697	The C. tropicalis pmr1Δ and och1Δ mutants	675:715	pmr1Δ	693:697	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	6	66	theme	increased	919:927	arg1	exposure					929:936	increased exposure	919:936	increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains	919:1010	These changes were accompanied by increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains.					
31849889	6	67	theme	chitin	962:967	arg1	exposure					929:936	increased exposure	919:936	increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains	919:1010	These changes were accompanied by increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains.					
31849889	8	68	theme	IL-10	1467:1471	arg1	production					1473:1482	IL-10 production	1467:1482	IL-10 production	1467:1482	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	5	69	theme	C.	679:680	arg1	pmr1Δ					693:697	The C. tropicalis pmr1Δ and och1Δ mutants	675:715	pmr1Δ	693:697	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	5	70	from	times	743:747	arg1	content					820:826	the wall mannan content	804:826	the wall mannan content	804:826	The C. tropicalis pmr1Δ and och1Δ mutants showed increased doubling times, aberrant colony and cellular morphology, reduction in the wall mannan content, and increased susceptibility to wall perturbing agents.					
31849889	6	71	theme	β1,3-glucan	946:956	arg1	exposure					929:936	increased exposure	919:936	increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains	919:1010	These changes were accompanied by increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains.					
31849889	3	72	link	O-linked	479:486	arg1	mannans					488:494	O-linked mannans	479:494	O-linked mannans	479:494	This cation is the cofactor of Golgi-resident mannosyltransferases, and thus Pmr1 is involved in the synthesis of both N- and O-linked mannans.					
31849889	2	73	from	α1,6-mannosyltransferase	191:214	arg1	albicans					170:177	Candida albicans	162:177	Candida albicans	162:177	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	8	74	theme	mannan	1312:1317	arg1	core					1319:1322	the N-linked mannan core	1299:1322	the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant	1299:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	8	74	theme	mannan	1312:1317	arg1	capable					1384:1390	capable	1384:1390	capable	1384:1390	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	7	75	link	O-linked	1037:1044	arg1	mannans					1046:1052	O-linked mannans	1037:1052	O-linked mannans	1037:1052	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	7	76	theme	N-linked	1126:1133	arg1	β1,3-glucan					1147:1157	β1,3-glucan	1147:1157	β1,3-glucan	1147:1157	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	7	76	theme	N-linked	1126:1133	arg1	ligands					1167:1173	key ligands	1163:1173	key ligands	1163:1173	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	7	76	theme	N-linked	1126:1133	arg1	mannans					1135:1141	N-linked mannans	1126:1141	N-linked mannans	1126:1141	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	8	77	theme	C.	1502:1503	arg1	interaction					1533:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	0	78	theme	Mannosylation	16:28	arg1	Role					0:3	Role	0:3	Role of Protein Mannosylation in the Candida tropicalis-Host Interaction.	0:72	Role of Protein Mannosylation in the Candida tropicalis-Host Interaction.					
31849889	6	79	theme	strains	1004:1010	arg1	surface					981:987	the wall surface	972:987	the wall surface of both mutant strains	972:1010	These changes were accompanied by increased exposure of both β1,3-glucan and chitin at the wall surface of both mutant strains.					
31849889	8	80	theme	cell	1528:1531	arg1	interaction					1533:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	the C. tropicalis-mononuclear cell interaction	1498:1543	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	7	81	theme	mononuclear	1103:1113	arg1	cells					1115:1119	human mononuclear cells	1097:1119	human mononuclear cells	1097:1119	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	7	82	theme	mannose	1257:1263	arg1	receptor					1265:1272	dectin-1 and mannose receptor	1244:1272	receptor	1265:1272	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	0	83	theme	tropicalis-Host	45:59	arg1	Interaction					61:71	the Candida tropicalis-Host Interaction	33:71	the Candida tropicalis-Host Interaction	33:71	Role of Protein Mannosylation in the Candida tropicalis-Host Interaction.					
31849889	9	84	theme	virulence	1573:1581	arg1	attenuation					1583:1593	virulence attenuation	1573:1593	virulence attenuation	1573:1593	Both mutant strains showed virulence attenuation in the Galleria mellonella and the mouse model of systemic candidiasis.					
31849889	7	85	theme	dectin-1	1244:1251	arg1	receptor					1265:1272	dectin-1 and mannose receptor	1244:1272	receptor	1265:1272	Our results showed that O-linked mannans are dispensable for cytokine production by human mononuclear cells, but N-linked mannans and β1,3-glucan are key ligands to trigger cytokine production in a co-stimulatory pathway involving dectin-1 and mannose receptor.					
31849889	8	86	theme	tropicalis	1351:1360	arg1	mutant					1373:1378	C. tropicalis och1Δ null mutant	1348:1378	C. tropicalis och1Δ null mutant	1348:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	2	87	theme	ion	304:306	arg1	pump					308:311	an ion pump	301:311	an ion pump that imports Mn2+ into the Golgi lumen	301:350	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	2	87	theme	ion	304:306	arg1	Pmr1					293:296	Pmr1	293:296	Pmr1	293:296	In Candida albicans, Och1 is an α1,6-mannosyltransferase that adds the first mannose unit to the N-linked mannan outer chain; whereas Pmr1 is an ion pump that imports Mn2+ into the Golgi lumen.					
31849889	8	88	theme	null	1368:1371	arg1	mutant					1373:1378	C. tropicalis och1Δ null mutant	1348:1378	C. tropicalis och1Δ null mutant	1348:1378	Moreover, we found that the N-linked mannan core found on the surface of C. tropicalis och1Δ null mutant was capable of inducing cytokine production; and that a mannan-independent pathway for IL-10 production is present in the C. tropicalis-mononuclear cell interaction.					
31849889	10	89	theme	cell	1703:1706	arg1	composition					1713:1723	cell wall composition	1703:1723	cell wall composition	1703:1723	Therefore, mannans are relevant for cell wall composition and organization, and for the C. tropicalis-host interaction.					
31849889	9	90	theme	Galleria	1602:1609	arg1	mellonella					1611:1620	the Galleria mellonella	1598:1620	the Galleria mellonella	1598:1620	Both mutant strains showed virulence attenuation in the Galleria mellonella and the mouse model of systemic candidiasis.					
31849889	3	91	theme	mannosyltransferases	399:418	arg1	cation					358:363	This cation	353:363	This cation	353:363	This cation is the cofactor of Golgi-resident mannosyltransferases, and thus Pmr1 is involved in the synthesis of both N- and O-linked mannans.					
31849889	3	91	theme	mannosyltransferases	399:418	arg1	cofactor					372:379	the cofactor	368:379	the cofactor of Golgi-resident mannosyltransferases	368:418	This cation is the cofactor of Golgi-resident mannosyltransferases, and thus Pmr1 is involved in the synthesis of both N- and O-linked mannans.					
31849889	1	92	attach	attached	116:123	arg2	wall					111:114	the fungal wall	100:114	the fungal wall attached to proteins via N- or O-linkages	100:156	Mannans are components of the fungal wall attached to proteins via N- or O-linkages.					
31849889	1	92	attach	attached	116:123	arg1	proteins					128:135	proteins	128:135	proteins	128:135	Mannans are components of the fungal wall attached to proteins via N- or O-linkages.					
31849889	3	93	theme	N-	472:473	arg1	synthesis					454:462	the synthesis	450:462	the synthesis of both N- and O-linked mannans	450:494	This cation is the cofactor of Golgi-resident mannosyltransferases, and thus Pmr1 is involved in the synthesis of both N- and O-linked mannans.					
31295499	5	0	theme	composite	735:743	arg1	hydrogels					756:764	composite HCS-DOPA-2 hydrogels	735:764	composite HCS-DOPA-2 hydrogels	735:764	In vitro antibacterial studies, composite HCS-DOPA-2 hydrogels exhibited lasting inhibition to S. aureus >8 h.					
31295499	6	1	theme	HCS-DOPA-2	895:904	arg1	hydrogels					916:924	HCS-DOPA-2 composite hydrogels	895:924	HCS-DOPA-2 composite hydrogels	895:924	The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis.					
31295499	1	2	theme	temperature-responsive	180:201	arg1	hydrogel					237:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel.					
31295499	5	3	theme	HCS-DOPA-2	745:754	arg1	hydrogels					756:764	composite HCS-DOPA-2 hydrogels	735:764	composite HCS-DOPA-2 hydrogels	735:764	In vitro antibacterial studies, composite HCS-DOPA-2 hydrogels exhibited lasting inhibition to S. aureus >8 h.					
31295499	2	4	theme	different	366:374	arg1	concentrations					376:389	different concentrations	366:389	different concentrations (0, 0.5, 1.0 and 2.0 mg/mL)	366:417	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively.					
31295499	2	4	theme	different	366:374	arg1	2.0 mg/mL					408:416	0, 0.5, 1.0 and 2.0 mg/mL	392:416	0, 0.5, 1.0 and 2.0 mg/mL	392:416	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively.					
31295499	6	5	theme	in	976:977	arg1	hemostasis					985:994	HCS in vitro hemostasis	972:994	HCS in vitro hemostasis	972:994	The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis.					
31295499	6	6	dep	in	976:977	arg1	vitro					979:983	vitro	979:983	vitro	979:983	The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis.					
31295499	2	7	theme	composite	293:301	arg1	hydrogels					303:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels	247:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels	247:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively.					
31295499	6	8	theme	HCS	972:974	arg1	hemostasis					985:994	HCS in vitro hemostasis	972:994	HCS in vitro hemostasis	972:994	The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis.					
31295499	3	9	theme	composite	555:563	arg1	CS					565:566	composite CS	555:566	composite CS	555:566	The gelling characteristic of HBC hydrogel was not influenced by composite CS and DOPA.					
31295499	5	10	theme	vitro	706:710	arg1	studies					726:732	vitro antibacterial studies	706:732	vitro antibacterial studies	706:732	In vitro antibacterial studies, composite HCS-DOPA-2 hydrogels exhibited lasting inhibition to S. aureus >8 h.					
31295499	6	11	theme	clotting	868:875	arg1	time					877:880	blood clotting time	862:880	blood clotting time treated with HCS-DOPA-2 composite hydrogels	862:924	The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis.					
31295499	7	12	theme	promising	1077:1085	arg1	hydrogels					1045:1053	HCS-DOPA-2 composite hydrogels	1024:1053	HCS-DOPA-2 composite hydrogels	1024:1053	The results suggested that HCS-DOPA-2 composite hydrogels could be applied as a promising wound dressing for hemostasis in vitro.					
31295499	7	12	theme	promising	1077:1085	arg1	dressing					1093:1100	a promising wound dressing	1075:1100	a promising wound dressing for hemostasis	1075:1115	The results suggested that HCS-DOPA-2 composite hydrogels could be applied as a promising wound dressing for hemostasis in vitro.					
31295499	3	13	theme	HBC	520:522	arg1	hydrogel					524:531	HBC hydrogel	520:531	HBC hydrogel	520:531	The gelling characteristic of HBC hydrogel was not influenced by composite CS and DOPA.					
31295499	1	14	theme	chitosan	216:223	arg1	hydrogel					237:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel.					
31295499	5	15	theme	antibacterial	712:724	arg1	studies					726:732	vitro antibacterial studies	706:732	vitro antibacterial studies	706:732	In vitro antibacterial studies, composite HCS-DOPA-2 hydrogels exhibited lasting inhibition to S. aureus >8 h.					
31295499	6	16	theme	blood	862:866	arg1	time					877:880	blood clotting time	862:880	blood clotting time treated with HCS-DOPA-2 composite hydrogels	862:924	The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis.					
31295499	7	17	theme	wound	1087:1091	arg1	hydrogels					1045:1053	HCS-DOPA-2 composite hydrogels	1024:1053	HCS-DOPA-2 composite hydrogels	1024:1053	The results suggested that HCS-DOPA-2 composite hydrogels could be applied as a promising wound dressing for hemostasis in vitro.					
31295499	7	17	theme	wound	1087:1091	arg1	dressing					1093:1100	a promising wound dressing	1075:1100	a promising wound dressing for hemostasis	1075:1115	The results suggested that HCS-DOPA-2 composite hydrogels could be applied as a promising wound dressing for hemostasis in vitro.					
31295499	2	18	from	concentrations	376:389	arg1	self-polymerization					343:361	the dopamine self-polymerization	330:361	the dopamine self-polymerization	330:361	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively.					
31295499	3	19	theme	hydrogel	524:531	arg1	characteristic					502:515	The gelling characteristic	490:515	The gelling characteristic of HBC hydrogel	490:531	The gelling characteristic of HBC hydrogel was not influenced by composite CS and DOPA.					
31295499	0	20	theme	antibacterial	16:28	arg1	polydopamine/chitosan/temperature-responsive					30:73	Mussel-inspired antibacterial polydopamine/chitosan/temperature-responsive	0:73	Mussel-inspired antibacterial polydopamine/chitosan/temperature-responsive	0:73	Mussel-inspired antibacterial polydopamine/chitosan/temperature-responsive hydrogels for rapid hemostasis.					
31295499	1	21	theme	study	123:127	arg1	aim					111:113	The aim	107:113	The aim of this study	107:127	The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel.					
31295499	1	22	theme	HBC	226:228	arg1	hydrogel					237:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel.					
31295499	0	23	theme	Mussel-inspired	0:14	arg1	polydopamine/chitosan/temperature-responsive					30:73	Mussel-inspired antibacterial polydopamine/chitosan/temperature-responsive	0:73	Mussel-inspired antibacterial polydopamine/chitosan/temperature-responsive	0:73	Mussel-inspired antibacterial polydopamine/chitosan/temperature-responsive hydrogels for rapid hemostasis.					
31295499	5	24	theme	aureus	801:806	arg1	>8 h					808:811	S. aureus >8 h	798:811	S. aureus >8 h	798:811	In vitro antibacterial studies, composite HCS-DOPA-2 hydrogels exhibited lasting inhibition to S. aureus >8 h.					
31295499	5	25	theme	S.	798:799	arg1	>8 h					808:811	S. aureus >8 h	798:811	S. aureus >8 h	798:811	In vitro antibacterial studies, composite HCS-DOPA-2 hydrogels exhibited lasting inhibition to S. aureus >8 h.					
31295499	1	26	theme	based	231:235	arg1	hydrogel					237:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel.					
31295499	6	27	dep	blood	824:828	arg1	test					830:833	test	830:833	test in vitro	830:842	The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis.					
31295499	1	28	theme	hydroxybutyl	203:214	arg1	hydrogel					237:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	178:244	The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel.					
31295499	2	29	dep	HBC	251:253	arg1	The					247:249	The	247:249	The	247:249	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively.					
31295499	0	30	theme	rapid	89:93	arg1	hemostasis					95:104	rapid hemostasis	89:104	rapid hemostasis	89:104	Mussel-inspired antibacterial polydopamine/chitosan/temperature-responsive hydrogels for rapid hemostasis.					
31295499	4	31	theme	5.0	682:684	arg1	%					685:685	%	685:685	%	685:685	The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate.					
31295499	4	32	theme	fibroblast	639:648	arg1	L929					657:660	L929	657:660	L929	657:660	The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate.					
31295499	4	32	theme	fibroblast	639:648	arg1	cells					650:654	mouse fibroblast cells	633:654	mouse fibroblast cells (L929)	633:661	The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate.					
31295499	2	33	theme	chitosan	257:264	arg1	hydrogels					303:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels	247:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels	247:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively.					
31295499	4	34	theme	mouse	633:637	arg1	L929					657:660	L929	657:660	L929	657:660	The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate.					
31295499	4	34	theme	mouse	633:637	arg1	cells					650:654	mouse fibroblast cells	633:654	mouse fibroblast cells (L929)	633:661	The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate.					
31295499	5	35	theme	lasting	776:782	arg1	inhibition					784:793	lasting inhibition	776:793	lasting inhibition to S. aureus >8 h	776:811	In vitro antibacterial studies, composite HCS-DOPA-2 hydrogels exhibited lasting inhibition to S. aureus >8 h.					
31295499	7	36	theme	HCS-DOPA-2	1024:1033	arg1	dressing					1093:1100	a promising wound dressing	1075:1100	a promising wound dressing for hemostasis	1075:1115	The results suggested that HCS-DOPA-2 composite hydrogels could be applied as a promising wound dressing for hemostasis in vitro.					
31295499	7	36	theme	HCS-DOPA-2	1024:1033	arg1	hydrogels					1045:1053	HCS-DOPA-2 composite hydrogels	1024:1053	HCS-DOPA-2 composite hydrogels	1024:1053	The results suggested that HCS-DOPA-2 composite hydrogels could be applied as a promising wound dressing for hemostasis in vitro.					
31295499	4	37	theme	%	685:685	arg1	rate					697:700	5.0% hemolysis rate	682:700	5.0% hemolysis rate	682:700	The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate.					
31295499	4	38	theme	HCS-DOPA	582:589	arg1	hydrogels					601:609	The HCS-DOPA composite hydrogels	578:609	The HCS-DOPA composite hydrogels	578:609	The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate.					
31295499	7	39	theme	composite	1035:1043	arg1	dressing					1093:1100	a promising wound dressing	1075:1100	a promising wound dressing for hemostasis	1075:1115	The results suggested that HCS-DOPA-2 composite hydrogels could be applied as a promising wound dressing for hemostasis in vitro.					
31295499	7	39	theme	composite	1035:1043	arg1	hydrogels					1045:1053	HCS-DOPA-2 composite hydrogels	1024:1053	HCS-DOPA-2 composite hydrogels	1024:1053	The results suggested that HCS-DOPA-2 composite hydrogels could be applied as a promising wound dressing for hemostasis in vitro.					
31295499	2	40	theme	HBC	251:253	arg1	hydrogels					303:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels	247:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels	247:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively.					
31295499	3	41	theme	gelling	494:500	arg1	characteristic					502:515	The gelling characteristic	490:515	The gelling characteristic of HBC hydrogel	490:531	The gelling characteristic of HBC hydrogel was not influenced by composite CS and DOPA.					
31295499	1	42	theme	effective	147:155	arg1	dressing					163:170	an effective wound dressing	144:170	an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	144:244	The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel.					
31295499	6	43	theme	whole	818:822	arg1	blood					824:828	The whole blood	814:828	The whole blood test in vitro	814:842	The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis.					
31295499	2	44	theme	dopamine	273:280	arg1	hydrogels					303:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels	247:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels	247:311	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively.					
31295499	1	45	theme	wound	157:161	arg1	dressing					163:170	an effective wound dressing	144:170	an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel	144:244	The aim of this study was to develop an effective wound dressing using a temperature-responsive hydroxybutyl chitosan (HBC) based hydrogel.					
31295499	4	46	theme	composite	591:599	arg1	hydrogels					601:609	The HCS-DOPA composite hydrogels	578:609	The HCS-DOPA composite hydrogels	578:609	The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate.					
31295499	4	47	theme	hemolysis	687:695	arg1	rate					697:700	5.0% hemolysis rate	682:700	5.0% hemolysis rate	682:700	The HCS-DOPA composite hydrogels were non-cytotoxic to mouse fibroblast cells (L929), and induced under 5.0% hemolysis rate.					
31295499	2	48	theme	dopamine	334:341	arg1	self-polymerization					343:361	the dopamine self-polymerization	330:361	the dopamine self-polymerization	330:361	The HBC - chitosan (CS) - dopamine (HCS-DOPA) composite hydrogels were prepared by the dopamine self-polymerization at different concentrations (0, 0.5, 1.0 and 2.0 mg/mL), termed as HCS, HCS-DOPA-0.5, HCS-DOPA-1 and HCS-DOPA-2, respectively.					
31295499	6	49	theme	composite	906:914	arg1	hydrogels					916:924	HCS-DOPA-2 composite hydrogels	895:924	HCS-DOPA-2 composite hydrogels	895:924	The whole blood test in vitro demonstrated that blood clotting time treated with HCS-DOPA-2 composite hydrogels was shortened to 95.6 s compared with that of HCS in vitro hemostasis.					
30738001	0	0	theme	zero-valent	85:95	arg1	composite					124:132	a zero-valent nano-silver/TiO2 -chitosan composite	83:132	a zero-valent nano-silver/TiO2 -chitosan composite	83:132	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.					
30738001	5	1	theme	CS-TiO2	718:724	arg1	inhibition					698:707	inhibition	698:707	inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum)	698:844	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	1	2	theme	silver	182:187	arg1	AgNPs					204:208	AgNPs	204:208	AgNPs	204:208	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	2	theme	silver	182:187	arg1	nanoparticles					189:201	zero-valent silver nanoparticles	170:201	zero-valent silver nanoparticles (AgNPs)	170:209	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	4	3	theme	infrared	555:562	arg1	spectroscopy					564:575	Fourier-transform infrared spectroscopy	537:575	Fourier-transform infrared spectroscopy	537:575	Fourier-transform infrared spectroscopy indicated that some AgNPs formed a chelate with CS through coordination of Ag+ with the CS amide II groups.					
30738001	0	4	from	disinfection	10:21	arg1	wards					71:75	hospital wards	62:75	hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite	62:132	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.					
30738001	6	5	theme	optimal	1160:1166	arg1	qF					1184:1185	qF	1184:1185	qF	1184:1185	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	6	5	theme	optimal	1160:1166	arg1	quality					1175:1181	the optimal filter quality	1156:1181	the optimal filter quality (qF)	1156:1186	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	6	6	theme	%	1260:1260	arg1	%					1266:1266	qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%	1227:1266	qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%	1227:1266	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	1	7	theme	nanoparticles	189:201	arg1	chitosan					233:240	chitosan	233:240	chitosan (CS)	233:245	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	7	theme	nanoparticles	189:201	arg1	titania					212:218	titania	212:218	titania (TiO2 )	212:226	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	7	theme	nanoparticles	189:201	arg1	nanoparticles					189:201	zero-valent silver nanoparticles	170:201	zero-valent silver nanoparticles (AgNPs)	170:209	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	7	theme	nanoparticles	189:201	arg1	TiO2					221:224	TiO2	221:224	TiO2	221:224	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	7	theme	nanoparticles	189:201	arg1	CS					243:244	CS	243:244	CS	243:244	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	7	theme	nanoparticles	189:201	arg1	composite					157:165	A novel antimicrobial composite	135:165	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs)	135:209	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	4	8	theme	Fourier-transform	537:553	arg1	spectroscopy					564:575	Fourier-transform infrared spectroscopy	537:575	Fourier-transform infrared spectroscopy	537:575	Fourier-transform infrared spectroscopy indicated that some AgNPs formed a chelate with CS through coordination of Ag+ with the CS amide II groups.					
30738001	0	9	theme	-chitosan	114:122	arg1	composite					124:132	a zero-valent nano-silver/TiO2 -chitosan composite	83:132	a zero-valent nano-silver/TiO2 -chitosan composite	83:132	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.					
30738001	1	10	theme	@	324:324	arg1	matrix					311:316	a CS-TiO2 matrix	301:316	a CS-TiO2 matrix (AgNPs@CS-TiO2 )	301:333	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	10	theme	@	324:324	arg1	CS-TiO2					325:331	AgNPs@CS-TiO2	319:331	AgNPs@CS-TiO2	319:331	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	11	from	AgNPs	292:296	arg1	matrix					311:316	a CS-TiO2 matrix	301:316	a CS-TiO2 matrix (AgNPs@CS-TiO2 )	301:333	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	11	from	AgNPs	292:296	arg1	CS-TiO2					325:331	AgNPs@CS-TiO2	319:331	AgNPs@CS-TiO2	319:331	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	5	12	theme	critical	911:918	arg1	concentrations					921:934	the minimum (critical) concentrations	898:934	the minimum (critical) concentrations of AgNPs	898:943	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	6	13	theme	TiO2	1078:1081	arg1	filter					1091:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	0	14	theme	nano-silver/TiO2	97:112	arg1	composite					124:132	a zero-valent nano-silver/TiO2 -chitosan composite	83:132	a zero-valent nano-silver/TiO2 -chitosan composite	83:132	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.					
30738001	8	15	theme	promising	1467:1475	arg1	potentials					1477:1486	promising potentials	1467:1486	promising potentials	1467:1486	Consequently, AgNPs@TiO2 -CS has promising potentials in bioaerosol purification.					
30738001	4	16	theme	amide	668:672	arg1	groups					677:682	the CS amide II groups	661:682	the CS amide II groups	661:682	Fourier-transform infrared spectroscopy indicated that some AgNPs formed a chelate with CS through coordination of Ag+ with the CS amide II groups.					
30738001	7	17	theme	-CS	1286:1288	arg1	filters					1294:1300	AgNPs@TiO2 -CS bed filters	1275:1300	AgNPs@TiO2 -CS bed filters	1275:1300	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	4	18	theme	CS	665:666	arg1	groups					677:682	the CS amide II groups	661:682	the CS amide II groups	661:682	Fourier-transform infrared spectroscopy indicated that some AgNPs formed a chelate with CS through coordination of Ag+ with the CS amide II groups.					
30738001	6	19	theme	@	1077:1077	arg1	filter					1091:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	1	20	from	deposition	278:287	arg1	matrix					311:316	a CS-TiO2 matrix	301:316	a CS-TiO2 matrix (AgNPs@CS-TiO2 )	301:333	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	20	from	deposition	278:287	arg1	CS-TiO2					325:331	AgNPs@CS-TiO2	319:331	AgNPs@CS-TiO2	319:331	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	8	21	contain	has	1463:1465	arg2	potentials					1477:1486	promising potentials	1467:1486	promising potentials	1467:1486	Consequently, AgNPs@TiO2 -CS has promising potentials in bioaerosol purification.					
30738001	8	21	contain	has	1463:1465	arg1	-CS					1459:1461	AgNPs@TiO2 -CS	1448:1461	AgNPs@TiO2 -CS	1448:1461	Consequently, AgNPs@TiO2 -CS has promising potentials in bioaerosol purification.					
30738001	7	22	theme	fungi	1396:1400	arg1	%					1371:1371	up to 88%	1363:1371	up to 88% of bacteria	1363:1383	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	7	22	theme	fungi	1396:1400	arg1	fungi					1396:1400	fungi	1396:1400	fungi	1396:1400	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	7	22	theme	fungi	1396:1400	arg1	bacteria					1376:1383	bacteria	1376:1383	bacteria	1376:1383	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	7	22	theme	fungi	1396:1400	arg1	%					1391:1391	97%	1389:1391	97% of fungi	1389:1400	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	2	23	theme	6.69-8.84 nm	433:444	arg1	diameters					420:428	diameters	420:428	diameters of 6.69-8.84 nm	420:444	Electron microscopy showed that the AgNPs were well dispersed on the CS-TiO2 , with diameters of 6.69-8.84 nm.					
30738001	0	24	theme	Effective	0:8	arg1	disinfection					10:21	Effective disinfection	0:21	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.	0:133	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.					
30738001	5	25	theme	inhibition	698:707	arg1	6.72-11.08					851:860	6.72-11.08	851:860	6.72-11.08	851:860	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	5	25	theme	inhibition	698:707	arg1	zones					689:693	The zones	685:693	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum)	685:844	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	7	26	theme	ventilation	1324:1334	arg1	systems					1336:1342	the ventilation systems	1320:1342	the ventilation systems of hospital wards	1320:1360	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	5	27	dep	bacteria	730:737	arg1	coli					752:755	Escherichia coli	740:755	Escherichia coli	740:755	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	5	27	dep	bacteria	730:737	arg1	epidermidis					776:786	Staphylococcus epidermidis	761:786	Staphylococcus epidermidis	761:786	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	7	28	dep	88	1369:1370	arg1	to					1366:1367	to	1366:1367	to	1366:1367	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	6	29	theme	filter	1091:1096	arg1	efficiency					1056:1065	The removal efficiency	1044:1065	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η)	1044:1116	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	4	30	theme	Ag+	652:654	arg1	coordination					636:647	coordination	636:647	coordination of Ag+ with the CS amide II groups	636:682	Fourier-transform infrared spectroscopy indicated that some AgNPs formed a chelate with CS through coordination of Ag+ with the CS amide II groups.					
30738001	1	31	theme	AgNPs	319:323	arg1	matrix					311:316	a CS-TiO2 matrix	301:316	a CS-TiO2 matrix (AgNPs@CS-TiO2 )	301:333	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	31	theme	AgNPs	319:323	arg1	CS-TiO2					325:331	AgNPs@CS-TiO2	319:331	AgNPs@CS-TiO2	319:331	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	6	32	theme	bed	1087:1089	arg1	filter					1091:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	0	33	theme	airborne	26:33	arg1	contamination					45:57	airborne microbial contamination	26:57	airborne microbial contamination	26:57	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.					
30738001	1	34	theme	photochemical	264:276	arg1	deposition					278:287	photochemical deposition	264:287	photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 )	264:333	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	6	35	theme	packing	1137:1143	arg1	depth					1145:1149	the packing depth	1133:1149	the packing depth	1133:1149	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	5	36	theme	minimum	902:908	arg1	concentrations					921:934	the minimum (critical) concentrations	898:934	the minimum (critical) concentrations of AgNPs	898:943	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	7	37	theme	bed	1290:1292	arg1	filters					1294:1300	AgNPs@TiO2 -CS bed filters	1275:1300	AgNPs@TiO2 -CS bed filters	1275:1300	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	3	38	theme	metallic	524:531	arg1	Ag					533:534	metallic Ag	524:534	metallic Ag	524:534	X-ray photoelectron spectra indicated that most of the AgNPs were reduced to metallic Ag.					
30738001	0	39	theme	contamination	45:57	arg1	disinfection					10:21	Effective disinfection	0:21	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.	0:133	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.					
30738001	6	40	theme	AgNPs	1072:1076	arg1	filter					1091:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	7	41	theme	@	1280:1280	arg1	filters					1294:1300	AgNPs@TiO2 -CS bed filters	1275:1300	AgNPs@TiO2 -CS bed filters	1275:1300	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	0	42	theme	microbial	35:43	arg1	contamination					45:57	airborne microbial contamination	26:57	airborne microbial contamination	26:57	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.					
30738001	1	43	theme	AgNPs	292:296	arg1	deposition					278:287	photochemical deposition	264:287	photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 )	264:333	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	6	44	theme	2-4 cm	1219:1224	arg1	depths					1209:1214	packing depths	1201:1214	packing depths of 2-4 cm	1201:1224	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	4	45	with	coordination	636:647	arg1	groups					677:682	the CS amide II groups	661:682	the CS amide II groups	661:682	Fourier-transform infrared spectroscopy indicated that some AgNPs formed a chelate with CS through coordination of Ag+ with the CS amide II groups.					
30738001	7	46	theme	hospital	1347:1354	arg1	wards					1356:1360	hospital wards	1347:1360	hospital wards	1347:1360	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	0	47	theme	hospital	62:69	arg1	wards					71:75	hospital wards	62:75	hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite	62:132	Effective disinfection of airborne microbial contamination in hospital wards using a zero-valent nano-silver/TiO2 -chitosan composite.					
30738001	5	48	theme	@	717:717	arg1	CS-TiO2					718:724	AgNPs@CS-TiO2	712:724	AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum)	712:844	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	3	49	theme	photoelectron	453:465	arg1	spectra					467:473	X-ray photoelectron spectra	447:473	X-ray photoelectron spectra	447:473	X-ray photoelectron spectra indicated that most of the AgNPs were reduced to metallic Ag.					
30738001	7	50	theme	wards	1356:1360	arg1	systems					1336:1342	the ventilation systems	1320:1342	the ventilation systems of hospital wards	1320:1360	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	6	51	theme	filter	1168:1173	arg1	qF					1184:1185	qF	1184:1185	qF	1184:1185	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	6	51	theme	filter	1168:1173	arg1	quality					1175:1181	the optimal filter quality	1156:1181	the optimal filter quality (qF)	1156:1186	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	1	52	theme	novel	137:141	arg1	composite					157:165	A novel antimicrobial composite	135:165	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs)	135:209	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	52	theme	novel	137:141	arg1	nanoparticles					189:201	zero-valent silver nanoparticles	170:201	zero-valent silver nanoparticles (AgNPs)	170:209	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	8	53	theme	bioaerosol	1491:1500	arg1	purification					1502:1513	bioaerosol purification	1491:1513	bioaerosol purification	1491:1513	Consequently, AgNPs@TiO2 -CS has promising potentials in bioaerosol purification.					
30738001	1	54	theme	CS-TiO2	303:309	arg1	matrix					311:316	a CS-TiO2 matrix	301:316	a CS-TiO2 matrix (AgNPs@CS-TiO2 )	301:333	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	54	theme	CS-TiO2	303:309	arg1	CS-TiO2					325:331	AgNPs@CS-TiO2	319:331	AgNPs@CS-TiO2	319:331	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	6	55	theme	packing	1201:1207	arg1	depths					1209:1214	packing depths	1201:1214	packing depths of 2-4 cm	1201:1224	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	5	56	theme	AgNPs	939:943	arg1	concentrations					921:934	the minimum (critical) concentrations	898:934	the minimum (critical) concentrations of AgNPs	898:943	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	7	57	theme	AgNPs	1275:1279	arg1	filters					1294:1300	AgNPs@TiO2 -CS bed filters	1275:1300	AgNPs@TiO2 -CS bed filters	1275:1300	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	1	58	theme	antimicrobial	143:155	arg1	composite					157:165	A novel antimicrobial composite	135:165	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs)	135:209	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	58	theme	antimicrobial	143:155	arg1	nanoparticles					189:201	zero-valent silver nanoparticles	170:201	zero-valent silver nanoparticles (AgNPs)	170:209	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	6	59	theme	-CS	1083:1085	arg1	filter					1091:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	a AgNPs@TiO2 -CS bed filter	1070:1096	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	7	60	theme	TiO2	1281:1284	arg1	filters					1294:1300	AgNPs@TiO2 -CS bed filters	1275:1300	AgNPs@TiO2 -CS bed filters	1275:1300	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	4	61	with	chelate	612:618	arg1	CS					625:626	CS	625:626	CS	625:626	Fourier-transform infrared spectroscopy indicated that some AgNPs formed a chelate with CS through coordination of Ag+ with the CS amide II groups.					
30738001	6	62	dep	occurred	1188:1195	arg1	%					1266:1266	qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%	1227:1266	qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%	1227:1266	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	3	63	theme	X-ray	447:451	arg1	spectra					467:473	X-ray photoelectron spectra	447:473	X-ray photoelectron spectra	447:473	X-ray photoelectron spectra indicated that most of the AgNPs were reduced to metallic Ag.					
30738001	8	64	theme	AgNPs	1448:1452	arg1	-CS					1459:1461	AgNPs@TiO2 -CS	1448:1461	AgNPs@TiO2 -CS	1448:1461	Consequently, AgNPs@TiO2 -CS has promising potentials in bioaerosol purification.					
30738001	5	65	theme	AgNPs	712:716	arg1	CS-TiO2					718:724	AgNPs@CS-TiO2	712:724	AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum)	712:844	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	6	66	theme	removal	1048:1054	arg1	efficiency					1056:1065	The removal efficiency	1044:1065	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η)	1044:1116	The removal efficiency of a AgNPs@TiO2 -CS bed filter for bioaerosols (η) increased with the packing depth, and the optimal filter quality (qF) occurred for packing depths of 2-4 cm (qF = 0.0285-0.103 Pa-1 ; η = 57.6%-98.2%).					
30738001	2	67	theme	Electron	336:343	arg1	microscopy					345:354	Electron microscopy	336:354	Electron microscopy	336:354	Electron microscopy showed that the AgNPs were well dispersed on the CS-TiO2 , with diameters of 6.69-8.84 nm.					
30738001	8	68	theme	TiO2	1454:1457	arg1	-CS					1459:1461	AgNPs@TiO2 -CS	1448:1461	AgNPs@TiO2 -CS	1448:1461	Consequently, AgNPs@TiO2 -CS has promising potentials in bioaerosol purification.					
30738001	1	69	from	matrix	311:316	arg1	deposition					278:287	photochemical deposition	264:287	photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 )	264:333	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	5	70	dep	fungi	793:797	arg1	niger					812:816	Aspergillus niger	800:816	Aspergillus niger	800:816	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	5	70	dep	fungi	793:797	arg1	spinulosum					834:843	Penicillium spinulosum	822:843	Penicillium spinulosum	822:843	The zones of inhibition of AgNPs@CS-TiO2 for bacteria (Escherichia coli and Staphylococcus epidermidis) and fungi (Aspergillus niger and Penicillium spinulosum) were 6.72-11.08 and 5.45-5.77 mm, respectively, and the minimum (critical) concentrations of AgNPs required to inhibit the growth of bacteria and fungi were 7.57 and 16.51 µg-Ag/mm2 , respectively.					
30738001	7	71	theme	bacteria	1376:1383	arg1	%					1371:1371	up to 88%	1363:1371	up to 88% of bacteria	1363:1383	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	7	71	theme	bacteria	1376:1383	arg1	fungi					1396:1400	fungi	1396:1400	fungi	1396:1400	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	7	71	theme	bacteria	1376:1383	arg1	bacteria					1376:1383	bacteria	1376:1383	bacteria	1376:1383	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	7	71	theme	bacteria	1376:1383	arg1	%					1391:1391	97%	1389:1391	97% of fungi	1389:1400	When AgNPs@TiO2 -CS bed filters were installed in the ventilation systems of hospital wards, up to 88% of bacteria and 97% of fungi were removed within 30 minutes.					
30738001	1	72	theme	zero-valent	170:180	arg1	AgNPs					204:208	AgNPs	204:208	AgNPs	204:208	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	1	72	theme	zero-valent	170:180	arg1	nanoparticles					189:201	zero-valent silver nanoparticles	170:201	zero-valent silver nanoparticles (AgNPs)	170:209	A novel antimicrobial composite of zero-valent silver nanoparticles (AgNPs), titania (TiO2 ), and chitosan (CS) was prepared via photochemical deposition of AgNPs on a CS-TiO2 matrix (AgNPs@CS-TiO2 ).					
30738001	8	73	theme	@	1453:1453	arg1	-CS					1459:1461	AgNPs@TiO2 -CS	1448:1461	AgNPs@TiO2 -CS	1448:1461	Consequently, AgNPs@TiO2 -CS has promising potentials in bioaerosol purification.					
31010593	7	0	theme	isolated	1222:1229	arg1	samples					1231:1237	freshly isolated samples	1214:1237	freshly isolated samples	1214:1237	Compared to freshly isolated samples, frozen preparation yielded small stiffness increases in each layer to varied degrees, thus inaccurately representing physiological stiffness.					
31010593	1	1	theme	vascular	263:270	arg1	physiology					277:286	local vascular cell physiology	257:286	local vascular cell physiology	257:286	The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology.					
31010593	9	2	theme	stiffness	1786:1794	arg1	increases					1796:1804	stiffness increases	1786:1804	stiffness increases in identical layers among different types of arteries	1786:1858	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	10	3	theme	composition	1870:1880	arg1	analysis					1882:1889	composition analysis	1870:1889	composition analysis	1870:1889	However, composition analysis was insufficient to interpret stiffness variations between layers which had dissimilar microstructure.					
31010593	6	4	theme	increased	1149:1157	arg1	stiffness					1159:1167	increased stiffness	1149:1167	increased stiffness	1149:1167	Adult samples, when compared to neonatal pulmonary arteries, exhibited increased stiffness in all layers except adventitia.					
31010593	3	5	theme	layer-specific	540:553	arg1	stiffness					555:563	layer-specific stiffness	540:563	layer-specific stiffness	540:563	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	3	5	theme	layer-specific	540:553	arg1	artery					652:657	carotid artery	644:657	carotid artery	644:657	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	3	5	theme	layer-specific	540:553	arg1	artery					615:620	the main pulmonary artery	596:620	the main pulmonary artery	596:620	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	3	5	theme	layer-specific	540:553	arg1	aorta					633:637	ascending aorta	623:637	ascending aorta	623:637	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	9	6	theme	identical	1809:1817	arg1	layers					1819:1824	identical layers	1809:1824	identical layers among different types of arteries	1809:1858	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	10	7	theme	stiffness	1921:1929	arg1	variations					1931:1940	stiffness variations	1921:1940	stiffness variations between layers which had dissimilar microstructure	1921:1991	However, composition analysis was insufficient to interpret stiffness variations between layers which had dissimilar microstructure.					
31010593	6	8	theme	pulmonary	1119:1127	arg1	arteries					1129:1136	neonatal pulmonary arteries	1110:1136	neonatal pulmonary arteries	1110:1136	Adult samples, when compared to neonatal pulmonary arteries, exhibited increased stiffness in all layers except adventitia.					
31010593	8	9	theme	structural	1462:1471	arg1	proteins					1480:1487	structural matrix proteins	1462:1487	structural matrix proteins	1462:1487	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	1	10	theme	intimate	230:237	arg1	role					239:242	the intimate role	226:242	the intimate role they play in local vascular cell physiology	226:286	The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology.					
31010593	4	11	theme	adult	883:887	arg1	aorta					889:893	the adult aorta	879:893	the adult aorta	879:893	To compare stiffness with age and frozen processing techniques, neonatal and adult pulmonary arteries were tested, while fresh (vibratomed) and frozen (cryotomed) tissues were tested from the adult aorta.					
31010593	8	12	theme	proteins	1480:1487	arg1	analyses					1450:1457	composition and structure analyses	1424:1457	composition and structure analyses of structural matrix proteins	1424:1487	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	3	13	theme	main	600:603	arg1	artery					615:620	the main pulmonary artery	596:620	the main pulmonary artery	596:620	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	9	14	theme	arteries	1851:1858	arg1	types					1842:1846	different types	1832:1846	different types of arteries	1832:1858	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	4	15	theme	processing	732:741	arg1	techniques					743:752	frozen processing techniques	725:752	frozen processing techniques	725:752	To compare stiffness with age and frozen processing techniques, neonatal and adult pulmonary arteries were tested, while fresh (vibratomed) and frozen (cryotomed) tissues were tested from the adult aorta.					
31010593	10	16	theme	dissimilar	1967:1976	arg1	microstructure					1978:1991	dissimilar microstructure	1967:1991	dissimilar microstructure	1967:1991	However, composition analysis was insufficient to interpret stiffness variations between layers which had dissimilar microstructure.					
31010593	2	17	from	variations	298:307	arg1	micromechanics					312:325	micromechanics	312:325	micromechanics among arterial layers (i.e. intima, media, adventitia)	312:380	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	2	17	from	variations	298:307	arg1	structure					440:448	structure	440:448	structure	440:448	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	2	17	from	variations	298:307	arg1	composition					421:431	local matrix composition	408:431	local matrix composition	408:431	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	8	18	theme	histology	1509:1517	arg1	modalities					1543:1552	histology and multiphoton imaging modalities	1509:1552	histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence	1509:1617	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	8	18	theme	histology	1509:1517	arg1	generation					1580:1589	second harmonic generation	1564:1589	second harmonic generation	1564:1589	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	8	18	theme	histology	1509:1517	arg1	fluorescence					1606:1617	two-photon fluorescence	1595:1617	two-photon fluorescence	1595:1617	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	0	19	theme	age	73:75	arg1	Influences					59:68	Influences	59:68	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.	0:123	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.					
31010593	3	20	theme	ascending	623:631	arg1	aorta					633:637	ascending aorta	623:637	ascending aorta	623:637	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	1	21	theme	micromechanics	150:163	arg1	importance					129:138	The importance	125:138	The importance of matrix micromechanics	125:163	The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology.					
31010593	8	22	theme	multiphoton	1523:1533	arg1	imaging					1535:1541	multiphoton imaging	1523:1541	multiphoton imaging	1523:1541	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	0	23	theme	anatomical	78:87	arg1	location					89:96	anatomical location	78:96	anatomical location	78:96	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.					
31010593	4	24	theme	pulmonary	774:782	arg1	arteries					784:791	neonatal and adult pulmonary arteries	755:791	neonatal and adult pulmonary arteries	755:791	To compare stiffness with age and frozen processing techniques, neonatal and adult pulmonary arteries were tested, while fresh (vibratomed) and frozen (cryotomed) tissues were tested from the adult aorta.					
31010593	8	25	theme	second	1564:1569	arg1	generation					1580:1589	second harmonic generation	1564:1589	second harmonic generation	1564:1589	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	0	26	theme	processing	103:112	arg1	technique					114:122	processing technique	103:122	processing technique	103:122	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.					
31010593	9	27	theme	Composition	1620:1630	arg1	analysis					1632:1639	Composition analysis	1620:1639	Composition analysis of matrix protein area density	1620:1670	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	9	28	from	decrease	1690:1697	arg1	ratios					1763:1768	the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios	1702:1768	the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios	1702:1768	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	2	29	from	location	462:469	arg1	micromechanics					312:325	micromechanics	312:325	micromechanics among arterial layers (i.e. intima, media, adventitia)	312:380	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	2	29	from	location	462:469	arg1	structure					440:448	structure	440:448	structure	440:448	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	2	29	from	location	462:469	arg1	composition					421:431	local matrix composition	408:431	local matrix composition	408:431	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	11	30	theme	complete	2050:2057	arg1	understanding					2059:2071	more complete understanding	2045:2071	more complete understanding of arterial micromechanics	2045:2098	Detailed microstructure analyses may contribute to more complete understanding of arterial micromechanics.					
31010593	0	31	theme	Layer-specific	0:13	arg1	micromechanics					24:37	Layer-specific arterial micromechanics	0:37	Layer-specific arterial micromechanics	0:37	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.					
31010593	5	32	theme	compressive	927:937	arg1	modulus					939:945	the mean compressive modulus	918:945	the mean compressive modulus	918:945	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	7	33	theme	varied	1310:1315	arg1	degrees					1317:1323	varied degrees	1310:1323	varied degrees	1310:1323	Compared to freshly isolated samples, frozen preparation yielded small stiffness increases in each layer to varied degrees, thus inaccurately representing physiological stiffness.					
31010593	8	34	theme	two-photon	1595:1604	arg1	fluorescence					1606:1617	two-photon fluorescence	1595:1617	two-photon fluorescence	1595:1617	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	2	35	dep	intima	355:360	arg1	i.e.					350:353	i.e.	350:353	i.e.	350:353	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	5	36	theme	media	1004:1008	arg1	layers					1026:1031	the intima, sub-luminal media, inner-middle media, and adventitia layers	960:1031	the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries	960:1075	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	2	37	theme	local	408:412	arg1	composition					421:431	local matrix composition	408:431	local matrix composition	408:431	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	9	38	theme	protein	1651:1657	arg1	density					1664:1670	matrix protein area density	1644:1670	matrix protein area density	1644:1670	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	11	39	theme	micromechanics	2085:2098	arg1	understanding					2059:2071	more complete understanding	2045:2071	more complete understanding of arterial micromechanics	2045:2098	Detailed microstructure analyses may contribute to more complete understanding of arterial micromechanics.					
31010593	9	40	theme	density	1664:1670	arg1	analysis					1632:1639	Composition analysis	1620:1639	Composition analysis of matrix protein area density	1620:1670	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	7	41	from	increases	1283:1291	arg1	layer					1301:1305	each layer	1296:1305	each layer to varied degrees	1296:1323	Compared to freshly isolated samples, frozen preparation yielded small stiffness increases in each layer to varied degrees, thus inaccurately representing physiological stiffness.					
31010593	2	42	theme	anatomical	451:460	arg1	location					462:469	anatomical location	451:469	anatomical location	451:469	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	3	43	theme	carotid	644:650	arg1	artery					652:657	carotid artery	644:657	carotid artery	644:657	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	8	44	theme	composition	1424:1434	arg1	analyses					1450:1457	composition and structure analyses	1424:1457	composition and structure analyses of structural matrix proteins	1424:1487	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	11	45	theme	microstructure	2003:2016	arg1	analyses					2018:2025	Detailed microstructure analyses	1994:2025	Detailed microstructure analyses	1994:2025	Detailed microstructure analyses may contribute to more complete understanding of arterial micromechanics.					
31010593	5	46	theme	adventitia	1015:1024	arg1	layers					1026:1031	the intima, sub-luminal media, inner-middle media, and adventitia layers	960:1031	the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries	960:1075	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	7	47	theme	stiffness	1273:1281	arg1	increases					1283:1291	small stiffness increases	1267:1291	small stiffness increases in each layer to varied degrees	1267:1323	Compared to freshly isolated samples, frozen preparation yielded small stiffness increases in each layer to varied degrees, thus inaccurately representing physiological stiffness.					
31010593	8	48	theme	structure	1440:1448	arg1	analyses					1450:1457	composition and structure analyses	1424:1457	composition and structure analyses of structural matrix proteins	1424:1487	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	5	49	theme	media	984:988	arg1	layers					1026:1031	the intima, sub-luminal media, inner-middle media, and adventitia layers	960:1031	the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries	960:1075	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	2	50	from	dependency	394:403	arg1	micromechanics					312:325	micromechanics	312:325	micromechanics among arterial layers (i.e. intima, media, adventitia)	312:380	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	2	50	from	dependency	394:403	arg1	structure					440:448	structure	440:448	structure	440:448	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	2	50	from	dependency	394:403	arg1	composition					421:431	local matrix composition	408:431	local matrix composition	408:431	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	3	51	theme	force	672:676	arg1	indentation					678:688	atomic force indentation	665:688	atomic force indentation	665:688	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	5	52	theme	inner-middle	991:1002	arg1	media					1004:1008	inner-middle media	991:1008	inner-middle media	991:1008	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	6	53	theme	Adult	1078:1082	arg1	samples					1084:1090	Adult samples	1078:1090	Adult samples	1078:1090	Adult samples, when compared to neonatal pulmonary arteries, exhibited increased stiffness in all layers except adventitia.					
31010593	5	54	from	layers	1026:1031	arg1	range					1040:1044	the range	1036:1044	the range of 1-10 kPa for adult arteries	1036:1075	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	1	55	theme	local	257:261	arg1	physiology					277:286	local vascular cell physiology	257:286	local vascular cell physiology	257:286	The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology.					
31010593	9	56	theme	glycosaminoglycan-to-collagen	1733:1761	arg1	ratios					1763:1768	the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios	1702:1768	the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios	1702:1768	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	1	57	theme	cell	272:275	arg1	physiology					277:286	local vascular cell physiology	257:286	local vascular cell physiology	257:286	The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology.					
31010593	4	58	theme	frozen	835:840	arg1	tissues					854:860	fresh (vibratomed) and frozen (cryotomed) tissues	812:860	tissues	854:860	To compare stiffness with age and frozen processing techniques, neonatal and adult pulmonary arteries were tested, while fresh (vibratomed) and frozen (cryotomed) tissues were tested from the adult aorta.					
31010593	2	59	theme	arterial	333:340	arg1	layers					342:347	arterial layers	333:347	arterial layers (i.e. intima, media, adventitia)	333:380	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	1	60	theme	cardiovascular	195:208	arg1	research					210:217	cardiovascular research	195:217	cardiovascular research due to the intimate role they play in local vascular cell physiology	195:286	The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology.					
31010593	9	61	from	increases	1796:1804	arg1	layers					1819:1824	identical layers	1809:1824	identical layers among different types of arteries	1809:1858	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	4	62	theme	cryotomed	843:851	arg1	tissues					854:860	fresh (vibratomed) and frozen (cryotomed) tissues	812:860	tissues	854:860	To compare stiffness with age and frozen processing techniques, neonatal and adult pulmonary arteries were tested, while fresh (vibratomed) and frozen (cryotomed) tissues were tested from the adult aorta.					
31010593	3	63	theme	elastic	568:574	arg1	arteries					576:583	elastic arteries	568:583	elastic arteries	568:583	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	1	64	theme	due	219:221	arg1	research					210:217	cardiovascular research	195:217	cardiovascular research due to the intimate role they play in local vascular cell physiology	195:286	The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology.					
31010593	2	65	from	stage	488:492	arg1	micromechanics					312:325	micromechanics	312:325	micromechanics among arterial layers (i.e. intima, media, adventitia)	312:380	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	2	65	from	stage	488:492	arg1	structure					440:448	structure	440:448	structure	440:448	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	2	65	from	stage	488:492	arg1	composition					421:431	local matrix composition	408:431	local matrix composition	408:431	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	6	66	theme	neonatal	1110:1117	arg1	arteries					1129:1136	neonatal pulmonary arteries	1110:1136	neonatal pulmonary arteries	1110:1136	Adult samples, when compared to neonatal pulmonary arteries, exhibited increased stiffness in all layers except adventitia.					
31010593	5	67	theme	1-10 kPa	1049:1056	arg1	range					1040:1044	the range	1036:1044	the range of 1-10 kPa for adult arteries	1036:1075	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	3	68	theme	pulmonary	605:613	arg1	artery					615:620	the main pulmonary artery	596:620	the main pulmonary artery	596:620	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	9	69	theme	different	1832:1840	arg1	types					1842:1846	different types	1832:1846	different types of arteries	1832:1858	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	1	70	dep	role	239:242	arg1	play					249:252	play	249:252	play in local vascular cell physiology	249:286	The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology.					
31010593	5	71	theme	adult	1062:1066	arg1	arteries					1068:1075	adult arteries	1062:1075	adult arteries	1062:1075	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	7	72	theme	physiological	1357:1369	arg1	stiffness					1371:1379	physiological stiffness	1357:1379	physiological stiffness	1357:1379	Compared to freshly isolated samples, frozen preparation yielded small stiffness increases in each layer to varied degrees, thus inaccurately representing physiological stiffness.					
31010593	4	73	theme	frozen	725:730	arg1	techniques					743:752	frozen processing techniques	725:752	frozen processing techniques	725:752	To compare stiffness with age and frozen processing techniques, neonatal and adult pulmonary arteries were tested, while fresh (vibratomed) and frozen (cryotomed) tissues were tested from the adult aorta.					
31010593	1	74	theme	matrix	143:148	arg1	micromechanics					150:163	matrix micromechanics	143:163	matrix micromechanics	143:163	The importance of matrix micromechanics is increasingly recognized in cardiovascular research due to the intimate role they play in local vascular cell physiology.					
31010593	8	75	theme	imaging	1535:1541	arg1	modalities					1543:1552	histology and multiphoton imaging modalities	1509:1552	histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence	1509:1617	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	8	75	theme	imaging	1535:1541	arg1	generation					1580:1589	second harmonic generation	1564:1589	second harmonic generation	1564:1589	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	8	75	theme	imaging	1535:1541	arg1	fluorescence					1606:1617	two-photon fluorescence	1595:1617	two-photon fluorescence	1595:1617	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	0	76	theme	location	89:96	arg1	Influences					59:68	Influences	59:68	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.	0:123	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.					
31010593	2	77	theme	developmental	474:486	arg1	stage					488:492	developmental stage	474:492	developmental stage	474:492	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	3	78	from	stiffness	555:563	arg1	arteries					576:583	elastic arteries	568:583	elastic arteries	568:583	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	8	79	theme	matrix	1473:1478	arg1	proteins					1480:1487	structural matrix proteins	1462:1487	structural matrix proteins	1462:1487	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	0	80	dep	micromechanics	24:37	arg1	Influences					59:68	Influences	59:68	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.	0:123	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.					
31010593	8	81	theme	harmonic	1571:1578	arg1	generation					1580:1589	second harmonic generation	1564:1589	second harmonic generation	1564:1589	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	4	82	theme	adult	768:772	arg1	arteries					784:791	neonatal and adult pulmonary arteries	755:791	neonatal and adult pulmonary arteries	755:791	To compare stiffness with age and frozen processing techniques, neonatal and adult pulmonary arteries were tested, while fresh (vibratomed) and frozen (cryotomed) tissues were tested from the adult aorta.					
31010593	11	83	theme	Detailed	1994:2001	arg1	analyses					2018:2025	Detailed microstructure analyses	1994:2025	Detailed microstructure analyses	1994:2025	Detailed microstructure analyses may contribute to more complete understanding of arterial micromechanics.					
31010593	0	84	theme	technique	114:122	arg1	Influences					59:68	Influences	59:68	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.	0:123	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.					
31010593	5	85	theme	intima	964:969	arg1	layers					1026:1031	the intima, sub-luminal media, inner-middle media, and adventitia layers	960:1031	the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries	960:1075	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	11	86	theme	arterial	2076:2083	arg1	micromechanics					2085:2098	arterial micromechanics	2076:2098	arterial micromechanics	2076:2098	Detailed microstructure analyses may contribute to more complete understanding of arterial micromechanics.					
31010593	0	87	theme	arterial	15:22	arg1	micromechanics					24:37	Layer-specific arterial micromechanics	0:37	Layer-specific arterial micromechanics	0:37	Layer-specific arterial micromechanics and microstructure: Influences of age, anatomical location, and processing technique.					
31010593	5	88	theme	mean	922:925	arg1	modulus					939:945	the mean compressive modulus	918:945	the mean compressive modulus	918:945	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	9	89	theme	matrix	1644:1649	arg1	density					1664:1670	matrix protein area density	1644:1670	matrix protein area density	1644:1670	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	2	90	theme	matrix	414:419	arg1	composition					421:431	local matrix composition	408:431	local matrix composition	408:431	However, variations in micromechanics among arterial layers (i.e. intima, media, adventitia), as well as dependency on local matrix composition and/or structure, anatomical location or developmental stage remain largely unknown.					
31010593	4	91	theme	neonatal	755:762	arg1	arteries					784:791	neonatal and adult pulmonary arteries	755:791	neonatal and adult pulmonary arteries	755:791	To compare stiffness with age and frozen processing techniques, neonatal and adult pulmonary arteries were tested, while fresh (vibratomed) and frozen (cryotomed) tissues were tested from the adult aorta.					
31010593	9	92	theme	area	1659:1662	arg1	density					1664:1670	matrix protein area density	1644:1670	matrix protein area density	1644:1670	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	10	93	contain	had	1963:1965	arg2	microstructure					1978:1991	dissimilar microstructure	1967:1991	dissimilar microstructure	1967:1991	However, composition analysis was insufficient to interpret stiffness variations between layers which had dissimilar microstructure.					
31010593	10	93	contain	had	1963:1965	arg1	layers					1950:1955	layers	1950:1955	layers which had dissimilar microstructure	1950:1991	However, composition analysis was insufficient to interpret stiffness variations between layers which had dissimilar microstructure.					
31010593	8	94	theme	micromechanics	1395:1408	arg1	measurements					1410:1421	micromechanics measurements	1395:1421	micromechanics measurements	1395:1421	To interpret micromechanics measurements, composition and structure analyses of structural matrix proteins were conducted with histology and multiphoton imaging modalities including second harmonic generation and two-photon fluorescence.					
31010593	7	95	theme	small	1267:1271	arg1	increases					1283:1291	small stiffness increases	1267:1291	small stiffness increases in each layer to varied degrees	1267:1323	Compared to freshly isolated samples, frozen preparation yielded small stiffness increases in each layer to varied degrees, thus inaccurately representing physiological stiffness.					
31010593	9	96	theme	elastin-to-collagen	1706:1724	arg1	ratios					1763:1768	the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios	1702:1768	the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios	1702:1768	Composition analysis of matrix protein area density demonstrated that decrease in the elastin-to-collagen and/or glycosaminoglycan-to-collagen ratios corresponded to stiffness increases in identical layers among different types of arteries.					
31010593	5	97	theme	sub-luminal	972:982	arg1	media					984:988	sub-luminal media	972:988	sub-luminal media	972:988	Results revealed that the mean compressive modulus varied among the intima, sub-luminal media, inner-middle media, and adventitia layers in the range of 1-10 kPa for adult arteries.					
31010593	3	98	theme	atomic	665:670	arg1	indentation					678:688	atomic force indentation	665:688	atomic force indentation	665:688	This study determined layer-specific stiffness in elastic arteries, including the main pulmonary artery, ascending aorta, and carotid artery using atomic force indentation.					
31010593	7	99	theme	frozen	1240:1245	arg1	preparation					1247:1257	frozen preparation	1240:1257	frozen preparation	1240:1257	Compared to freshly isolated samples, frozen preparation yielded small stiffness increases in each layer to varied degrees, thus inaccurately representing physiological stiffness.					
31074612	6	0	theme	useful	1116:1121	arg1	approach					1123:1130	a useful approach	1114:1130	a useful approach for designing multifunctional self-healing protein-based hydrogels	1114:1197	This study not only shows great potential in expanding the role of silk-based devices for various applications but also provides a useful approach for designing multifunctional self-healing protein-based hydrogels.					
31074612	5	1	from	applications	908:919	arg1	possibility					834:844	possibility	834:844	possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials	834:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	1	2	theme	polypyrrole	277:287	arg1	nature					267:272	the conductive nature	252:272	the conductive nature of polypyrrole (PPy)	252:293	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	1	2	theme	polypyrrole	277:287	arg1	nature					213:218	the dynamic assembly/disassembly nature	180:218	the dynamic assembly/disassembly nature of supramolecular complexes	180:246	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	2	3	theme	self-healing	300:311	arg1	properties					313:322	The self-healing properties	296:322	The self-healing properties of the hydrogels	296:339	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	6	4	theme	great	1011:1015	arg1	potential					1017:1025	great potential	1011:1025	great potential	1011:1025	This study not only shows great potential in expanding the role of silk-based devices for various applications but also provides a useful approach for designing multifunctional self-healing protein-based hydrogels.					
31074612	3	5	theme	PPy	497:499	arg1	deposition					501:510	PPy deposition	497:510	PPy deposition	497:510	PPy deposition was achieved via in situ polymerization of pyrrole using ammonium persulfate as an oxidant and laccase as a catalyst.					
31074612	4	6	theme	adjustable	731:740	arg1	conductivities					753:766	adjustable electrical conductivities	731:766	adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1	731:818	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	4	7	theme	PPy-coated	634:643	arg1	hydrogels					645:653	The PPy-coated hydrogels	630:653	The PPy-coated hydrogels	630:653	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	1	8	theme	Self-healing	81:92	arg1	hydrogels					146:154	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels	81:154	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels	81:154	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	1	9	theme	dynamic	184:190	arg1	nature					213:218	the dynamic assembly/disassembly nature	180:218	the dynamic assembly/disassembly nature of supramolecular complexes	180:246	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	3	10	dep	oxidant	595:601	arg1	an					592:593	an	592:593	an	592:593	PPy deposition was achieved via in situ polymerization of pyrrole using ammonium persulfate as an oxidant and laccase as a catalyst.					
31074612	2	11	theme	side	425:428	arg1	tyrosine					438:445	tyrosine	438:445	tyrosine	438:445	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	11	theme	side	425:428	arg1	chains					430:435	amino acid side chains	414:435	amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine)	414:488	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	11	theme	side	425:428	arg1	phenylalanine					460:472	phenylalanine	460:472	phenylalanine	460:472	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	11	theme	side	425:428	arg1	histidine					479:487	histidine	479:487	histidine	479:487	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	11	theme	side	425:428	arg1	tryptophan					448:457	tryptophan	448:457	tryptophan	448:457	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	12	theme	hydrogels	331:339	arg1	properties					313:322	The self-healing properties	296:322	The self-healing properties of the hydrogels	296:339	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	13	theme	acid	420:423	arg1	tyrosine					438:445	tyrosine	438:445	tyrosine	438:445	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	13	theme	acid	420:423	arg1	chains					430:435	amino acid side chains	414:435	amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine)	414:488	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	13	theme	acid	420:423	arg1	phenylalanine					460:472	phenylalanine	460:472	phenylalanine	460:472	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	13	theme	acid	420:423	arg1	histidine					479:487	histidine	479:487	histidine	479:487	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	13	theme	acid	420:423	arg1	tryptophan					448:457	tryptophan	448:457	tryptophan	448:457	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	4	14	theme	electrical	742:751	arg1	conductivities					753:766	adjustable electrical conductivities	731:766	adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1	731:818	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	5	15	theme	utilization	859:869	arg1	possibility					834:844	possibility	834:844	possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials	834:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	1	16	theme	conductive	111:120	arg1	hydrogels					146:154	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels	81:154	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels	81:154	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	1	17	theme	assembly/disassembly	192:211	arg1	nature					213:218	the dynamic assembly/disassembly nature	180:218	the dynamic assembly/disassembly nature of supramolecular complexes	180:246	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	4	18	theme	×	806:806	arg1	S·cm-1					813:818	(1.0 ± 0.3) × 10-3 S·cm-1	794:818	(1.0 ± 0.3) × 10-3 S·cm-1	794:818	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	4	18	theme	×	806:806	arg1	±					799:799	1.0 ± 0.3	795:803	1.0 ± 0.3	795:803	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	5	19	theme	electrode	951:959	arg1	materials					961:969	flexible yet self-healable electrode materials	924:969	flexible yet self-healable electrode materials	924:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	3	20	theme	ammonium	569:576	arg1	persulfate					578:587	ammonium persulfate	569:587	ammonium persulfate	569:587	PPy deposition was achieved via in situ polymerization of pyrrole using ammonium persulfate as an oxidant and laccase as a catalyst.					
31074612	6	21	theme	various	1075:1081	arg1	applications					1083:1094	various applications	1075:1094	various applications	1075:1094	This study not only shows great potential in expanding the role of silk-based devices for various applications but also provides a useful approach for designing multifunctional self-healing protein-based hydrogels.					
31074612	0	22	theme	Self-Healing	10:21	arg1	Hydrogels					70:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Design of Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels.					
31074612	4	23	theme	electrical	703:712	arg1	properties					714:723	excellent electrical properties	693:723	excellent electrical properties	693:723	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	4	23	theme	electrical	703:712	arg1	elastomer					669:677	an elastomer	666:677	an elastomer	666:677	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	0	24	theme	Conductive	40:49	arg1	Hydrogels					70:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Design of Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels.					
31074612	6	25	theme	devices	1063:1069	arg1	role					1044:1047	the role	1040:1047	the role of silk-based devices for various applications	1040:1094	This study not only shows great potential in expanding the role of silk-based devices for various applications but also provides a useful approach for designing multifunctional self-healing protein-based hydrogels.					
31074612	2	26	theme	amino	414:418	arg1	tyrosine					438:445	tyrosine	438:445	tyrosine	438:445	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	26	theme	amino	414:418	arg1	chains					430:435	amino acid side chains	414:435	amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine)	414:488	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	26	theme	amino	414:418	arg1	phenylalanine					460:472	phenylalanine	460:472	phenylalanine	460:472	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	26	theme	amino	414:418	arg1	histidine					479:487	histidine	479:487	histidine	479:487	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	26	theme	amino	414:418	arg1	tryptophan					448:457	tryptophan	448:457	tryptophan	448:457	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	1	27	theme	supramolecular	223:236	arg1	complexes					238:246	supramolecular complexes	223:246	supramolecular complexes	223:246	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	0	28	theme	Electrically	27:38	arg1	Hydrogels					70:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Design of Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels.					
31074612	4	29	theme	10-3	808:811	arg1	S·cm-1					813:818	(1.0 ± 0.3) × 10-3 S·cm-1	794:818	(1.0 ± 0.3) × 10-3 S·cm-1	794:818	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	4	29	theme	10-3	808:811	arg1	±					799:799	1.0 ± 0.3	795:803	1.0 ± 0.3	795:803	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	5	30	theme	potential	849:857	arg1	utilization					859:869	potential utilization	849:869	potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials	849:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	1	31	theme	complexes	238:246	arg1	nature					267:272	the conductive nature	252:272	the conductive nature of polypyrrole (PPy)	252:293	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	1	31	theme	complexes	238:246	arg1	nature					213:218	the dynamic assembly/disassembly nature	180:218	the dynamic assembly/disassembly nature of supramolecular complexes	180:246	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	0	32	theme	Fibroin-Based	56:68	arg1	Hydrogels					70:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Design of Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels.					
31074612	6	33	theme	silk-based	1052:1061	arg1	devices					1063:1069	silk-based devices	1052:1069	silk-based devices	1052:1069	This study not only shows great potential in expanding the role of silk-based devices for various applications but also provides a useful approach for designing multifunctional self-healing protein-based hydrogels.					
31074612	2	34	theme	host-guest	363:372	arg1	interactions					374:385	host-guest interactions	363:385	host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine)	363:488	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	0	35	theme	Silk	51:54	arg1	Hydrogels					70:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels	10:78	Design of Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels.					
31074612	6	36	theme	self-healing	1162:1173	arg1	hydrogels					1189:1197	multifunctional self-healing protein-based hydrogels	1146:1197	multifunctional self-healing protein-based hydrogels	1146:1197	This study not only shows great potential in expanding the role of silk-based devices for various applications but also provides a useful approach for designing multifunctional self-healing protein-based hydrogels.					
31074612	5	37	theme	hydrogels	878:886	arg1	utilization					859:869	potential utilization	849:869	potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials	849:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	5	38	from	possibility	834:844	arg1	applications					908:919	electrochemistry applications	891:919	electrochemistry applications as flexible yet self-healable electrode materials	891:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	5	39	theme	self-healable	937:949	arg1	materials					961:969	flexible yet self-healable electrode materials	924:969	flexible yet self-healable electrode materials	924:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	3	40	dep	in	529:530	arg1	situ					532:535	situ	532:535	situ	532:535	PPy deposition was achieved via in situ polymerization of pyrrole using ammonium persulfate as an oxidant and laccase as a catalyst.					
31074612	5	41	theme	flexible	924:931	arg1	materials					961:969	flexible yet self-healable electrode materials	924:969	flexible yet self-healable electrode materials	924:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	3	42	theme	pyrrole	555:561	arg1	polymerization					537:550	in situ polymerization	529:550	in situ polymerization of pyrrole using ammonium persulfate as an oxidant and laccase as a catalyst	529:627	PPy deposition was achieved via in situ polymerization of pyrrole using ammonium persulfate as an oxidant and laccase as a catalyst.					
31074612	1	43	theme	-based	139:144	arg1	hydrogels					146:154	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels	81:154	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels	81:154	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	6	44	theme	protein-based	1175:1187	arg1	hydrogels					1189:1197	multifunctional self-healing protein-based hydrogels	1146:1197	multifunctional self-healing protein-based hydrogels	1146:1197	This study not only shows great potential in expanding the role of silk-based devices for various applications but also provides a useful approach for designing multifunctional self-healing protein-based hydrogels.					
31074612	6	45	theme	multifunctional	1146:1160	arg1	hydrogels					1189:1197	multifunctional self-healing protein-based hydrogels	1146:1197	multifunctional self-healing protein-based hydrogels	1146:1197	This study not only shows great potential in expanding the role of silk-based devices for various applications but also provides a useful approach for designing multifunctional self-healing protein-based hydrogels.					
31074612	5	46	theme	electrochemistry	891:906	arg1	applications					908:919	electrochemistry applications	891:919	electrochemistry applications as flexible yet self-healable electrode materials	891:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	2	47	dep	chains	430:435	arg1	tyrosine					438:445	tyrosine	438:445	tyrosine	438:445	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	47	dep	chains	430:435	arg1	chains					430:435	amino acid side chains	414:435	amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine)	414:488	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	47	dep	chains	430:435	arg1	phenylalanine					460:472	phenylalanine	460:472	phenylalanine	460:472	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	47	dep	chains	430:435	arg1	histidine					479:487	histidine	479:487	histidine	479:487	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	2	47	dep	chains	430:435	arg1	tryptophan					448:457	tryptophan	448:457	tryptophan	448:457	The self-healing properties of the hydrogels were achieved through host-guest interactions between β-cyclodextrin and amino acid side chains (tyrosine, tryptophan, phenylalanine, and histidine) on SF.					
31074612	3	48	theme	in	529:530	arg1	polymerization					537:550	in situ polymerization	529:550	in situ polymerization of pyrrole using ammonium persulfate as an oxidant and laccase as a catalyst	529:627	PPy deposition was achieved via in situ polymerization of pyrrole using ammonium persulfate as an oxidant and laccase as a catalyst.					
31074612	1	49	theme	conductive	256:265	arg1	nature					267:272	the conductive nature	252:272	the conductive nature of polypyrrole (PPy)	252:293	Self-healing and electrically conductive silk fibroin (SF)-based hydrogels were developed based on the dynamic assembly/disassembly nature of supramolecular complexes and the conductive nature of polypyrrole (PPy).					
31074612	0	50	theme	Hydrogels	70:78	arg1	Design					0:5	Design	0:5	Design of Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels.	0:79	Design of Self-Healing and Electrically Conductive Silk Fibroin-Based Hydrogels.					
31074612	5	51	from	utilization	859:869	arg1	applications					908:919	electrochemistry applications	891:919	electrochemistry applications as flexible yet self-healable electrode materials	891:969	Furthermore, possibility of potential utilization of the hydrogels in electrochemistry applications as flexible yet self-healable electrode materials was explored.					
31074612	4	52	theme	excellent	693:701	arg1	properties					714:723	excellent electrical properties	693:723	excellent electrical properties	693:723	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31074612	4	52	theme	excellent	693:701	arg1	elastomer					669:677	an elastomer	666:677	an elastomer	666:677	The PPy-coated hydrogels behaved as an elastomer and displayed excellent electrical properties, with adjustable electrical conductivities ranging from 0.8 ± 0.2 to (1.0 ± 0.3) × 10-3 S·cm-1.					
31318010	8	0	theme	cycle	1084:1088	arg1	arrest					1090:1095	cell cycle arrest	1079:1095	cell cycle arrest at the G2/M phase	1079:1113	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	3	1	theme	milk	322:325	arg1	oligosaccharides					328:343	oligosaccharides	328:343	oligosaccharides	328:343	As multifunctional components of milk, oligosaccharides have been reported to have the potential to support intestine development.					
31318010	3	1	theme	milk	322:325	arg1	components					308:317	multifunctional components	292:317	multifunctional components of milk	292:325	As multifunctional components of milk, oligosaccharides have been reported to have the potential to support intestine development.					
31318010	9	2	theme	infant	1219:1224	arg1	nutrition					1226:1234	infant nutrition	1219:1234	infant nutrition	1219:1234	Our research will help understand the biological functions of DMOs and assess their potential roles in infant nutrition.					
31318010	1	3	theme	Donkey	160:165	arg1	milk					167:170	Donkey milk	160:170	Donkey milk	160:170	Donkey milk is considered to be a valuable nutritional source.					
31318010	1	3	theme	Donkey	160:165	arg1	source					215:220	a valuable nutritional source	192:220	a valuable nutritional source	192:220	Donkey milk is considered to be a valuable nutritional source.					
31318010	9	4	theme	biological	1154:1163	arg1	functions					1165:1173	the biological functions	1150:1173	the biological functions of DMOs	1150:1181	Our research will help understand the biological functions of DMOs and assess their potential roles in infant nutrition.					
31318010	0	5	theme	growth	110:115	arg1	arrest					117:122	G2/M growth arrest	105:122	G2/M growth arrest	105:122	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	8	6	from	phase	1109:1113	arg1	arrest					1090:1095	cell cycle arrest	1079:1095	cell cycle arrest at the G2/M phase	1079:1113	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	8	6	from	phase	1109:1113	arg1	activation					1045:1054	activation	1045:1054	activation of the p38 pathway	1045:1073	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	7	7	from	proliferation	820:832	arg1	cells					860:864	HT-29, Caco-2 and HIEC cells	837:864	HT-29, Caco-2 and HIEC cells in a concentration-dependent manner	837:900	Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells.					
31318010	6	8	dep	mg	711:712	arg1	L-1					714:716	L-1	714:716	33.1 ± 0.7 mg L-1	700:716	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	0	9	theme	G2/M	105:108	arg1	arrest					117:122	G2/M growth arrest	105:122	G2/M growth arrest	105:122	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	4	10	theme	oligosaccharides	474:489	arg1	content					451:457	content	451:457	content	451:457	We studied the composition and content of donkey milk oligosaccharides (DMOs).					
31318010	4	10	theme	oligosaccharides	474:489	arg1	composition					435:445	composition	435:445	composition	435:445	We studied the composition and content of donkey milk oligosaccharides (DMOs).					
31318010	8	11	theme	G2/M	1104:1107	arg1	phase					1109:1113	the G2/M phase	1100:1113	the G2/M phase	1100:1113	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	4	12	theme	milk	469:472	arg1	DMOs					492:495	DMOs	492:495	DMOs	492:495	We studied the composition and content of donkey milk oligosaccharides (DMOs).					
31318010	4	12	theme	milk	469:472	arg1	oligosaccharides					474:489	donkey milk oligosaccharides	462:489	donkey milk oligosaccharides (DMOs)	462:496	We studied the composition and content of donkey milk oligosaccharides (DMOs).					
31318010	8	13	theme	effects	1002:1008	arg1	mechanism					979:987	The mechanism	975:987	The mechanism of the DMOs' effects on HT-29 cells	975:1023	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	2	14	theme	milk	270:273	arg1	constituents					247:258	the constituents	243:258	the constituents of donkey milk	243:273	Deeper knowledge of the constituents of donkey milk is necessary.					
31318010	6	15	theme	±	683:683	arg1	mg					689:690	18.3 ± 0.7 mg	678:690	18.3 ± 0.7 mg L-1	678:694	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	15	theme	±	683:683	arg1	3'-SL					643:647	3'-SL	643:647	3'-SL	643:647	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	15	theme	±	683:683	arg1	6'-SL					653:657	6'-SL	653:657	6'-SL	653:657	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	15	theme	±	683:683	arg1	amount					633:638	The amount	629:638	The amount of 3'-SL and 6'-SL in donkey milk	629:672	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	7	16	theme	concentration-dependent	871:893	arg1	manner					895:900	a concentration-dependent manner	869:900	a concentration-dependent manner	869:900	Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells.					
31318010	2	17	theme	donkey	263:268	arg1	milk					270:273	donkey milk	263:273	donkey milk	263:273	Deeper knowledge of the constituents of donkey milk is necessary.					
31318010	5	18	theme	Sialylated	499:508	arg1	oligosaccharides					556:571	the primary oligosaccharides	544:571	the primary oligosaccharides	544:571	Sialylated oligosaccharides were found to be the primary oligosaccharides in DMOs, consisting of 3'-sialyllactose (SL) and 6'-SL.					
31318010	5	18	theme	Sialylated	499:508	arg1	oligosaccharides					510:525	Sialylated oligosaccharides	499:525	Sialylated oligosaccharides	499:525	Sialylated oligosaccharides were found to be the primary oligosaccharides in DMOs, consisting of 3'-sialyllactose (SL) and 6'-SL.					
31318010	0	19	theme	milk	7:10	arg1	oligosaccharides					12:27	Donkey milk oligosaccharides	0:27	Donkey milk oligosaccharides	0:27	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	8	20	theme	cell	1079:1082	arg1	arrest					1090:1095	cell cycle arrest	1079:1095	cell cycle arrest at the G2/M phase	1079:1113	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	8	21	theme	HT-29	1013:1017	arg1	cells					1019:1023	HT-29 cells	1013:1023	HT-29 cells	1013:1023	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	3	22	contain	have	367:370	arg1	oligosaccharides					328:343	oligosaccharides	328:343	oligosaccharides	328:343	As multifunctional components of milk, oligosaccharides have been reported to have the potential to support intestine development.					
31318010	3	22	contain	have	367:370	arg1	components					308:317	multifunctional components	292:317	multifunctional components of milk	292:325	As multifunctional components of milk, oligosaccharides have been reported to have the potential to support intestine development.					
31318010	3	22	contain	have	367:370	arg2	potential					376:384	the potential to support intestine development	372:417	the potential to support intestine development	372:417	As multifunctional components of milk, oligosaccharides have been reported to have the potential to support intestine development.					
31318010	0	23	theme	Donkey	0:5	arg1	oligosaccharides					12:27	Donkey milk oligosaccharides	0:27	Donkey milk oligosaccharides	0:27	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	0	24	theme	p38	132:134	arg1	pathway					136:142	the p38 pathway	128:142	the p38 pathway in HT-29 cells	128:157	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	6	25	theme	donkey	662:667	arg1	milk					669:672	donkey milk	662:672	donkey milk	662:672	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	5	26	from	oligosaccharides	556:571	arg1	DMOs					576:579	DMOs	576:579	DMOs	576:579	Sialylated oligosaccharides were found to be the primary oligosaccharides in DMOs, consisting of 3'-sialyllactose (SL) and 6'-SL.					
31318010	9	27	theme	DMOs	1178:1181	arg1	functions					1165:1173	the biological functions	1150:1173	the biological functions of DMOs	1150:1181	Our research will help understand the biological functions of DMOs and assess their potential roles in infant nutrition.					
31318010	7	28	from	cells	860:864	arg1	manner					895:900	a concentration-dependent manner	869:900	a concentration-dependent manner	869:900	Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells.					
31318010	0	29	theme	growth-related	43:56	arg1	characteristics					58:72	the growth-related characteristics	39:72	the growth-related characteristics of intestinal cells	39:92	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	8	30	from	mechanism	979:987	arg1	cells					1019:1023	HT-29 cells	1013:1023	HT-29 cells	1013:1023	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	6	31	theme	6'-SL	653:657	arg1	6'-SL					653:657	6'-SL	653:657	6'-SL	653:657	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	31	theme	6'-SL	653:657	arg1	3'-SL					643:647	3'-SL	643:647	3'-SL	643:647	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	31	theme	6'-SL	653:657	arg1	mg					689:690	18.3 ± 0.7 mg	678:690	18.3 ± 0.7 mg L-1	678:694	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	31	theme	6'-SL	653:657	arg1	amount					633:638	The amount	629:638	The amount of 3'-SL and 6'-SL in donkey milk	629:672	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	1	32	theme	valuable	194:201	arg1	source					215:220	a valuable nutritional source	192:220	a valuable nutritional source	192:220	Donkey milk is considered to be a valuable nutritional source.					
31318010	1	32	theme	valuable	194:201	arg1	milk					167:170	Donkey milk	160:170	Donkey milk	160:170	Donkey milk is considered to be a valuable nutritional source.					
31318010	4	33	theme	donkey	462:467	arg1	DMOs					492:495	DMOs	492:495	DMOs	492:495	We studied the composition and content of donkey milk oligosaccharides (DMOs).					
31318010	4	33	theme	donkey	462:467	arg1	oligosaccharides					474:489	donkey milk oligosaccharides	462:489	donkey milk oligosaccharides (DMOs)	462:496	We studied the composition and content of donkey milk oligosaccharides (DMOs).					
31318010	2	34	theme	Deeper	223:228	arg1	knowledge					230:238	Deeper knowledge	223:238	Deeper knowledge of the constituents of donkey milk	223:273	Deeper knowledge of the constituents of donkey milk is necessary.					
31318010	1	35	theme	nutritional	203:213	arg1	source					215:220	a valuable nutritional source	192:220	a valuable nutritional source	192:220	Donkey milk is considered to be a valuable nutritional source.					
31318010	1	35	theme	nutritional	203:213	arg1	milk					167:170	Donkey milk	160:170	Donkey milk	160:170	Donkey milk is considered to be a valuable nutritional source.					
31318010	9	36	from	roles	1210:1214	arg1	nutrition					1226:1234	infant nutrition	1219:1234	infant nutrition	1219:1234	Our research will help understand the biological functions of DMOs and assess their potential roles in infant nutrition.					
31318010	3	37	theme	multifunctional	292:306	arg1	oligosaccharides					328:343	oligosaccharides	328:343	oligosaccharides	328:343	As multifunctional components of milk, oligosaccharides have been reported to have the potential to support intestine development.					
31318010	3	37	theme	multifunctional	292:306	arg1	components					308:317	multifunctional components	292:317	multifunctional components of milk	292:325	As multifunctional components of milk, oligosaccharides have been reported to have the potential to support intestine development.					
31318010	8	38	theme	pathway	1067:1073	arg1	arrest					1090:1095	cell cycle arrest	1079:1095	cell cycle arrest at the G2/M phase	1079:1113	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	8	38	theme	pathway	1067:1073	arg1	activation					1045:1054	activation	1045:1054	activation of the p38 pathway	1045:1073	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	5	39	theme	primary	548:554	arg1	oligosaccharides					556:571	the primary oligosaccharides	544:571	the primary oligosaccharides	544:571	Sialylated oligosaccharides were found to be the primary oligosaccharides in DMOs, consisting of 3'-sialyllactose (SL) and 6'-SL.					
31318010	5	39	theme	primary	548:554	arg1	oligosaccharides					510:525	Sialylated oligosaccharides	499:525	Sialylated oligosaccharides	499:525	Sialylated oligosaccharides were found to be the primary oligosaccharides in DMOs, consisting of 3'-sialyllactose (SL) and 6'-SL.					
31318010	3	40	theme	intestine	397:405	arg1	development					407:417	intestine development	397:417	intestine development	397:417	As multifunctional components of milk, oligosaccharides have been reported to have the potential to support intestine development.					
31318010	8	41	from	effects	1002:1008	arg1	cells					1019:1023	HT-29 cells	1013:1023	HT-29 cells	1013:1023	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	0	42	theme	HT-29	147:151	arg1	cells					153:157	HT-29 cells	147:157	HT-29 cells	147:157	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	7	43	theme	epithelial	957:966	arg1	cells					968:972	intestinal epithelial cells	946:972	intestinal epithelial cells	946:972	Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells.					
31318010	7	44	theme	HIEC	855:858	arg1	cells					860:864	HT-29, Caco-2 and HIEC cells	837:864	HT-29, Caco-2 and HIEC cells in a concentration-dependent manner	837:900	Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells.					
31318010	8	45	theme	p38	1063:1065	arg1	pathway					1067:1073	the p38 pathway	1059:1073	the p38 pathway	1059:1073	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
31318010	6	46	theme	±	705:705	arg1	mg					711:712	33.1 ± 0.7 mg	700:712	33.1 ± 0.7 mg L-1	700:716	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	47	theme	3'-SL	643:647	arg1	6'-SL					653:657	6'-SL	653:657	6'-SL	653:657	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	47	theme	3'-SL	643:647	arg1	3'-SL					643:647	3'-SL	643:647	3'-SL	643:647	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	47	theme	3'-SL	643:647	arg1	mg					689:690	18.3 ± 0.7 mg	678:690	18.3 ± 0.7 mg L-1	678:694	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	6	47	theme	3'-SL	643:647	arg1	amount					633:638	The amount	629:638	The amount of 3'-SL and 6'-SL in donkey milk	629:672	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	4	48	dep	composition	435:445	arg1	the					431:433	the	431:433	the	431:433	We studied the composition and content of donkey milk oligosaccharides (DMOs).					
31318010	7	49	theme	cells	968:972	arg1	maturation					932:941	maturation	932:941	maturation of intestinal epithelial cells	932:972	Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells.					
31318010	2	50	theme	constituents	247:258	arg1	knowledge					230:238	Deeper knowledge	223:238	Deeper knowledge of the constituents of donkey milk	223:273	Deeper knowledge of the constituents of donkey milk is necessary.					
31318010	6	51	from	amount	633:638	arg1	milk					669:672	donkey milk	662:672	donkey milk	662:672	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	7	52	theme	Caco-2	844:849	arg1	cells					860:864	HT-29, Caco-2 and HIEC cells	837:864	HT-29, Caco-2 and HIEC cells in a concentration-dependent manner	837:900	Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells.					
31318010	0	53	from	pathway	136:142	arg1	cells					153:157	HT-29 cells	147:157	HT-29 cells	147:157	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	0	54	theme	cells	88:92	arg1	characteristics					58:72	the growth-related characteristics	39:72	the growth-related characteristics of intestinal cells	39:92	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	9	55	theme	potential	1200:1208	arg1	roles					1210:1214	their potential roles	1194:1214	their potential roles in infant nutrition	1194:1234	Our research will help understand the biological functions of DMOs and assess their potential roles in infant nutrition.					
31318010	7	56	theme	intestinal	946:955	arg1	cells					968:972	intestinal epithelial cells	946:972	intestinal epithelial cells	946:972	Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells.					
31318010	6	57	dep	mg	689:690	arg1	L-1					692:694	L-1	692:694	18.3 ± 0.7 mg L-1	678:694	The amount of 3'-SL and 6'-SL in donkey milk was 18.3 ± 0.7 mg L-1 and 33.1 ± 0.7 mg L-1, respectively.					
31318010	0	58	theme	intestinal	77:86	arg1	cells					88:92	intestinal cells	77:92	intestinal cells	77:92	Donkey milk oligosaccharides influence the growth-related characteristics of intestinal cells and induce G2/M growth arrest via the p38 pathway in HT-29 cells.					
31318010	7	59	theme	HT-29	837:841	arg1	cells					860:864	HT-29, Caco-2 and HIEC cells	837:864	HT-29, Caco-2 and HIEC cells in a concentration-dependent manner	837:900	Moreover, we found that DMOs induced differentiation, promoted apoptosis and inhibited proliferation in HT-29, Caco-2 and HIEC cells in a concentration-dependent manner, suggesting that DMOs promote maturation of intestinal epithelial cells.					
31318010	8	60	from	cells	1019:1023	arg1	mechanism					979:987	The mechanism	975:987	The mechanism of the DMOs' effects on HT-29 cells	975:1023	The mechanism of the DMOs' effects on HT-29 cells was associated with activation of the p38 pathway and cell cycle arrest at the G2/M phase.					
29280476	3	0	theme	chitosan	329:336	arg1	polymers					338:345	chitosan polymers	329:345	chitosan polymers	329:345	One method to produce paCOS is partial chemical hydrolysis of chitosan polymers, but that leads to poorly defined mixtures of oligosaccharides.					
29280476	4	1	theme	developing	506:515	arg1	applications					517:528	developing applications	506:528	developing applications	506:528	However, the effective production of defined paCOS is crucial for fundamental research and for developing applications.					
29280476	9	2	theme	N-acetyl-D-glucosamine	1104:1125	arg1	units					1127:1131	N-acetyl-D-glucosamine units	1104:1131	N-acetyl-D-glucosamine units	1104:1131	Two muteins with active site substitutions can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wildtype enzyme.					
29280476	3	3	theme	polymers	338:345	arg1	hydrolysis					315:324	partial chemical hydrolysis	298:324	partial chemical hydrolysis of chitosan polymers	298:345	One method to produce paCOS is partial chemical hydrolysis of chitosan polymers, but that leads to poorly defined mixtures of oligosaccharides.					
29280476	2	4	theme	chitosan	113:120	arg1	paCOS					140:144	paCOS	140:144	paCOS	140:144	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	2	4	theme	chitosan	113:120	arg1	oligosaccharides					122:137	Partially acetylated chitosan oligosaccharides	92:137	Partially acetylated chitosan oligosaccharides (paCOS)	92:145	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	9	5	with	muteins	1054:1060	arg1	substitutions					1079:1091	active site substitutions	1067:1091	active site substitutions	1067:1091	Two muteins with active site substitutions can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wildtype enzyme.					
29280476	2	6	theme	potential	160:168	arg1	applications					170:181	various potential applications	152:181	various potential applications in agriculture, biomedicine, and pharmaceutics	152:228	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	6	7	theme	enzymes	760:766	arg1	Protein-engineering					731:749	Protein-engineering	731:749	Protein-engineering of these enzymes to alter their substrate specificity	731:803	Protein-engineering of these enzymes to alter their substrate specificity can overcome the limitations associated with naturally occurring enzymes and expand the spectrum of specific paCOS that can be produced.					
29280476	4	8	theme	fundamental	477:487	arg1	research					489:496	fundamental research	477:496	fundamental research	477:496	However, the effective production of defined paCOS is crucial for fundamental research and for developing applications.					
29280476	5	9	theme	substrate	640:648	arg1	specificity					650:660	the substrate specificity	636:660	the substrate specificity of the enzyme	636:674	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	0	10	from	sp	49:50	arg1	Protein-engineering					0:18	Protein-engineering	0:18	Protein-engineering of chitosanase from Bacillus sp.	0:51	Protein-engineering of chitosanase from Bacillus sp.					
29280476	7	11	theme	sp	998:999	arg1	specificity					974:984	the substrate specificity	960:984	the substrate specificity of Bacillus sp	960:999	Here, engineering the substrate specificity of Bacillus sp.					
29280476	1	12	theme	substrate	69:77	arg1	specificity					79:89	its substrate specificity	65:89	its substrate specificity	65:89	MN to alter its substrate specificity.					
29280476	9	13	theme	active	1067:1072	arg1	substitutions					1079:1091	active site substitutions	1067:1091	active site substitutions	1067:1091	Two muteins with active site substitutions can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wildtype enzyme.					
29280476	2	14	theme	various	152:158	arg1	applications					170:181	various potential applications	152:181	various potential applications in agriculture, biomedicine, and pharmaceutics	152:228	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	3	15	theme	oligosaccharides	393:408	arg1	mixtures					381:388	poorly defined mixtures	366:388	poorly defined mixtures of oligosaccharides	366:408	One method to produce paCOS is partial chemical hydrolysis of chitosan polymers, but that leads to poorly defined mixtures of oligosaccharides.					
29280476	5	16	theme	enzyme	669:674	arg1	specificity					650:660	the substrate specificity	636:660	the substrate specificity of the enzyme	636:674	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	3	17	theme	defined	373:379	arg1	mixtures					381:388	poorly defined mixtures	366:388	poorly defined mixtures of oligosaccharides	366:408	One method to produce paCOS is partial chemical hydrolysis of chitosan polymers, but that leads to poorly defined mixtures of oligosaccharides.					
29280476	6	18	theme	occurring	860:868	arg1	enzymes					870:876	naturally occurring enzymes	850:876	naturally occurring enzymes	850:876	Protein-engineering of these enzymes to alter their substrate specificity can overcome the limitations associated with naturally occurring enzymes and expand the spectrum of specific paCOS that can be produced.					
29280476	0	19	theme	chitosanase	23:33	arg1	Protein-engineering					0:18	Protein-engineering	0:18	Protein-engineering of chitosanase from Bacillus sp.	0:51	Protein-engineering of chitosanase from Bacillus sp.					
29280476	4	20	theme	paCOS	456:460	arg1	crucial					465:471	crucial	465:471	crucial	465:471	However, the effective production of defined paCOS is crucial for fundamental research and for developing applications.					
29280476	4	20	theme	paCOS	456:460	arg1	production					434:443	the effective production	420:443	the effective production of defined paCOS	420:460	However, the effective production of defined paCOS is crucial for fundamental research and for developing applications.					
29280476	2	21	theme	suitable	243:250	arg1	bioactivities					252:264	their suitable bioactivities	237:264	their suitable bioactivities	237:264	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	8	22	theme	MN	1002:1003	arg1	chitosanase					1005:1015	MN chitosanase	1002:1015	MN chitosanase	1002:1015	MN chitosanase is described for the first time.					
29280476	9	23	theme	wildtype	1184:1191	arg1	enzyme					1193:1198	the wildtype enzyme	1180:1198	the wildtype enzyme	1180:1198	Two muteins with active site substitutions can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wildtype enzyme.					
29280476	0	24	theme	Bacillus	40:47	arg1	sp					49:50	Bacillus sp	40:50	Bacillus sp	40:50	Protein-engineering of chitosanase from Bacillus sp.					
29280476	2	25	theme	acetylated	102:111	arg1	paCOS					140:144	paCOS	140:144	paCOS	140:144	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	2	25	theme	acetylated	102:111	arg1	oligosaccharides					122:137	Partially acetylated chitosan oligosaccharides	92:137	Partially acetylated chitosan oligosaccharides (paCOS)	92:145	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	5	26	theme	enzymatic	560:568	arg1	approach					548:555	A more promising approach	531:555	A more promising approach	531:555	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	5	26	theme	enzymatic	560:568	arg1	depolymerization					570:585	enzymatic depolymerization	560:585	enzymatic depolymerization of chitosan using chitinases or chitosanases	560:630	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	6	27	theme	paCOS	914:918	arg1	spectrum					893:900	the spectrum	889:900	the spectrum of specific paCOS that can be produced	889:939	Protein-engineering of these enzymes to alter their substrate specificity can overcome the limitations associated with naturally occurring enzymes and expand the spectrum of specific paCOS that can be produced.					
29280476	5	28	theme	products	721:728	arg1	composition					691:701	the composition	687:701	the composition of the oligomeric products	687:728	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	5	29	theme	oligomeric	710:719	arg1	products					721:728	the oligomeric products	706:728	the oligomeric products	706:728	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	6	30	theme	specific	905:912	arg1	paCOS					914:918	specific paCOS	905:918	specific paCOS that can be produced	905:939	Protein-engineering of these enzymes to alter their substrate specificity can overcome the limitations associated with naturally occurring enzymes and expand the spectrum of specific paCOS that can be produced.					
29280476	7	31	theme	substrate	964:972	arg1	specificity					974:984	the substrate specificity	960:984	the substrate specificity of Bacillus sp	960:999	Here, engineering the substrate specificity of Bacillus sp.					
29280476	7	32	theme	Bacillus	989:996	arg1	sp					998:999	Bacillus sp	989:999	Bacillus sp	989:999	Here, engineering the substrate specificity of Bacillus sp.					
29280476	3	33	theme	partial	298:304	arg1	hydrolysis					315:324	partial chemical hydrolysis	298:324	partial chemical hydrolysis of chitosan polymers	298:345	One method to produce paCOS is partial chemical hydrolysis of chitosan polymers, but that leads to poorly defined mixtures of oligosaccharides.					
29280476	4	34	theme	effective	424:432	arg1	crucial					465:471	crucial	465:471	crucial	465:471	However, the effective production of defined paCOS is crucial for fundamental research and for developing applications.					
29280476	4	34	theme	effective	424:432	arg1	production					434:443	the effective production	420:443	the effective production of defined paCOS	420:460	However, the effective production of defined paCOS is crucial for fundamental research and for developing applications.					
29280476	5	35	theme	chitosan	590:597	arg1	approach					548:555	A more promising approach	531:555	A more promising approach	531:555	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	5	35	theme	chitosan	590:597	arg1	depolymerization					570:585	enzymatic depolymerization	560:585	enzymatic depolymerization of chitosan using chitinases or chitosanases	560:630	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	8	36	theme	first	1038:1042	arg1	time					1044:1047	the first time	1034:1047	the first time	1034:1047	MN chitosanase is described for the first time.					
29280476	9	37	theme	site	1074:1077	arg1	substitutions					1079:1091	active site substitutions	1067:1091	active site substitutions	1067:1091	Two muteins with active site substitutions can accept N-acetyl-D-glucosamine units at their subsite (-2), which is impossible for the wildtype enzyme.					
29280476	3	38	theme	chemical	306:313	arg1	hydrolysis					315:324	partial chemical hydrolysis	298:324	partial chemical hydrolysis of chitosan polymers	298:345	One method to produce paCOS is partial chemical hydrolysis of chitosan polymers, but that leads to poorly defined mixtures of oligosaccharides.					
29280476	4	39	theme	defined	448:454	arg1	paCOS					456:460	defined paCOS	448:460	defined paCOS	448:460	However, the effective production of defined paCOS is crucial for fundamental research and for developing applications.					
29280476	6	40	theme	substrate	783:791	arg1	specificity					793:803	their substrate specificity	777:803	their substrate specificity	777:803	Protein-engineering of these enzymes to alter their substrate specificity can overcome the limitations associated with naturally occurring enzymes and expand the spectrum of specific paCOS that can be produced.					
29280476	5	41	theme	promising	538:546	arg1	approach					548:555	A more promising approach	531:555	A more promising approach	531:555	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	5	41	theme	promising	538:546	arg1	depolymerization					570:585	enzymatic depolymerization	560:585	enzymatic depolymerization of chitosan using chitinases or chitosanases	560:630	A more promising approach is enzymatic depolymerization of chitosan using chitinases or chitosanases, as the substrate specificity of the enzyme determines the composition of the oligomeric products.					
29280476	2	42	from	applications	170:181	arg1	agriculture					186:196	agriculture	186:196	agriculture	186:196	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	2	42	from	applications	170:181	arg1	biomedicine					199:209	biomedicine	199:209	biomedicine	199:209	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	2	42	from	applications	170:181	arg1	pharmaceutics					216:228	pharmaceutics	216:228	pharmaceutics	216:228	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	2	43	contain	have	147:150	arg2	applications					170:181	various potential applications	152:181	various potential applications in agriculture, biomedicine, and pharmaceutics	152:228	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	2	43	contain	have	147:150	arg1	paCOS					140:144	paCOS	140:144	paCOS	140:144	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
29280476	2	43	contain	have	147:150	arg1	oligosaccharides					122:137	Partially acetylated chitosan oligosaccharides	92:137	Partially acetylated chitosan oligosaccharides (paCOS)	92:145	Partially acetylated chitosan oligosaccharides (paCOS) have various potential applications in agriculture, biomedicine, and pharmaceutics due to their suitable bioactivities.					
31518954	0	0	theme	improved	87:94	arg1	properties					125:134	improved mechanical and antibacterial properties	87:134	improved mechanical and antibacterial properties	87:134	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	5	1	theme	mechanical	762:771	arg1	properties					773:782	higher mechanical properties	755:782	higher mechanical properties	755:782	The films showed thermal stability and higher mechanical properties as compared to pure PVA films.					
31518954	1	2	theme	properties	172:181	arg1	combination					141:151	The combination	137:151	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films	137:270	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	8	3	theme	biomedical	1084:1093	arg1	fields					1095:1100	biomedical fields	1084:1100	biomedical fields	1084:1100	We believe that these antibacterial films may find applications in active food packaging and biomedical fields.					
31518954	6	4	theme	3T3	897:899	arg1	cells					918:922	NIH 3T3 mouse fibroblast cells	893:922	NIH 3T3 mouse fibroblast cells	893:922	In addition, the cytotoxicity of the films was evaluated by MTT assay against NIH 3T3 mouse fibroblast cells.					
31518954	6	5	theme	NIH	893:895	arg1	cells					918:922	NIH 3T3 mouse fibroblast cells	893:922	NIH 3T3 mouse fibroblast cells	893:922	In addition, the cytotoxicity of the films was evaluated by MTT assay against NIH 3T3 mouse fibroblast cells.					
31518954	2	6	theme	bacterial	354:362	arg1	cellulose					364:372	bacterial cellulose	354:372	bacterial cellulose (BC)	354:377	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	2	6	theme	bacterial	354:362	arg1	BC					375:376	BC	375:376	BC	375:376	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	1	7	theme	based	260:264	arg1	films					266:270	the polyvinyl alcohol (PVA) based films	232:270	the polyvinyl alcohol (PVA) based films	232:270	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	2	8	contain	containing	343:352	arg1	films					337:341	PVA films	333:341	PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL)	333:407	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	2	8	contain	containing	343:352	arg2	BC					375:376	BC	375:376	BC	375:376	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	2	8	contain	containing	343:352	arg2	cellulose					364:372	bacterial cellulose	354:372	bacterial cellulose (BC)	354:377	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	2	8	contain	containing	343:352	arg2	ε-PL					403:406	ε-PL	403:406	ε-PL	403:406	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	2	8	contain	containing	343:352	arg2	epsilon-polylysine					383:400	epsilon-polylysine	383:400	epsilon-polylysine (ε-PL)	383:407	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	8	9	theme	antibacterial	1013:1025	arg1	films					1027:1031	these antibacterial films	1007:1031	these antibacterial films	1007:1031	We believe that these antibacterial films may find applications in active food packaging and biomedical fields.					
31518954	3	10	theme	antibacterial	495:507	arg1	properties					509:518	more than 99% antibacterial properties	481:518	more than 99% antibacterial properties	481:518	The prepared composite films showed more than 99% antibacterial properties against both Staphylococcus aureus and Escherichia coli bacteria.					
31518954	1	11	theme	films	266:270	arg1	synthesis					219:227	a green synthesis	211:227	a green synthesis of the polyvinyl alcohol (PVA) based films	211:270	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	1	11	theme	films	266:270	arg1	properties					172:181	high mechanical properties	156:181	high mechanical properties	156:181	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	1	11	theme	films	266:270	arg1	activity					198:205	antibacterial activity	184:205	antibacterial activity	184:205	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	0	12	theme	mechanical	96:105	arg1	properties					125:134	improved mechanical and antibacterial properties	87:134	improved mechanical and antibacterial properties	87:134	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	0	13	with	ε-polylysine	69:80	arg1	properties					125:134	improved mechanical and antibacterial properties	87:134	improved mechanical and antibacterial properties	87:134	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	4	14	theme	strong	685:690	arg1	activity					706:713	strong antibacterial activity	685:713	strong antibacterial activity	685:713	Moreover, the films were collected after a single use and were reused twice, which still exhibited strong antibacterial activity.					
31518954	5	15	theme	thermal	733:739	arg1	stability					741:749	thermal stability	733:749	thermal stability	733:749	The films showed thermal stability and higher mechanical properties as compared to pure PVA films.					
31518954	6	16	theme	MTT	875:877	arg1	assay					879:883	MTT assay	875:883	MTT assay	875:883	In addition, the cytotoxicity of the films was evaluated by MTT assay against NIH 3T3 mouse fibroblast cells.					
31518954	3	17	theme	composite	458:466	arg1	films					468:472	The prepared composite films	445:472	The prepared composite films	445:472	The prepared composite films showed more than 99% antibacterial properties against both Staphylococcus aureus and Escherichia coli bacteria.					
31518954	1	18	theme	antibacterial	184:196	arg1	activity					198:205	antibacterial activity	184:205	antibacterial activity	184:205	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	0	19	theme	antibacterial	111:123	arg1	properties					125:134	improved mechanical and antibacterial properties	87:134	improved mechanical and antibacterial properties	87:134	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	5	20	theme	PVA	804:806	arg1	films					808:812	pure PVA films	799:812	pure PVA films	799:812	The films showed thermal stability and higher mechanical properties as compared to pure PVA films.					
31518954	4	21	theme	single	629:634	arg1	use					636:638	a single use	627:638	a single use	627:638	Moreover, the films were collected after a single use and were reused twice, which still exhibited strong antibacterial activity.					
31518954	2	22	theme	casting	429:435	arg1	method					437:442	a green solution casting method	412:442	a green solution casting method	412:442	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	1	23	theme	activity	198:205	arg1	combination					141:151	The combination	137:151	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films	137:270	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	0	24	theme	ternary	9:15	arg1	films					21:25	Reusable ternary PVA films	0:25	Reusable ternary PVA films	0:25	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	0	25	theme	Reusable	0:7	arg1	films					21:25	Reusable ternary PVA films	0:25	Reusable ternary PVA films	0:25	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	2	26	theme	ternary	315:321	arg1	system					323:328	a ternary system	313:328	a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL)	313:407	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	2	27	theme	solution	420:427	arg1	method					437:442	a green solution casting method	412:442	a green solution casting method	412:442	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	8	28	theme	active	1058:1063	arg1	packaging					1070:1078	active food packaging	1058:1078	active food packaging	1058:1078	We believe that these antibacterial films may find applications in active food packaging and biomedical fields.					
31518954	1	29	theme	green	213:217	arg1	synthesis					219:227	a green synthesis	211:227	a green synthesis of the polyvinyl alcohol (PVA) based films	211:270	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	0	30	theme	PVA	17:19	arg1	films					21:25	Reusable ternary PVA films	0:25	Reusable ternary PVA films	0:25	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	3	31	dep	properties	509:518	arg1	%					493:493	%	493:493	%	493:493	The prepared composite films showed more than 99% antibacterial properties against both Staphylococcus aureus and Escherichia coli bacteria.					
31518954	1	32	theme	synthesis	219:227	arg1	combination					141:151	The combination	137:151	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films	137:270	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	0	33	theme	bacterial	38:46	arg1	fibers					58:63	bacterial cellulose fibers	38:63	bacterial cellulose fibers	38:63	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	4	34	theme	antibacterial	692:704	arg1	activity					706:713	strong antibacterial activity	685:713	strong antibacterial activity	685:713	Moreover, the films were collected after a single use and were reused twice, which still exhibited strong antibacterial activity.					
31518954	6	35	theme	films	852:856	arg1	cytotoxicity					832:843	the cytotoxicity	828:843	the cytotoxicity of the films	828:856	In addition, the cytotoxicity of the films was evaluated by MTT assay against NIH 3T3 mouse fibroblast cells.					
31518954	8	36	theme	food	1065:1068	arg1	packaging					1070:1078	active food packaging	1058:1078	active food packaging	1058:1078	We believe that these antibacterial films may find applications in active food packaging and biomedical fields.					
31518954	7	37	theme	films	963:967	arg1	toxicity					947:954	no toxicity	944:954	no toxicity of the films towards tested cells	944:988	The results showed no toxicity of the films towards tested cells.					
31518954	5	38	theme	pure	799:802	arg1	films					808:812	pure PVA films	799:812	pure PVA films	799:812	The films showed thermal stability and higher mechanical properties as compared to pure PVA films.					
31518954	0	39	theme	cellulose	48:56	arg1	fibers					58:63	bacterial cellulose fibers	38:63	bacterial cellulose fibers	38:63	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	3	40	dep	%	493:493	arg1	99					491:492	99	491:492	99	491:492	The prepared composite films showed more than 99% antibacterial properties against both Staphylococcus aureus and Escherichia coli bacteria.					
31518954	7	41	theme	tested	977:982	arg1	cells					984:988	tested cells	977:988	tested cells	977:988	The results showed no toxicity of the films towards tested cells.					
31518954	2	42	theme	films	337:341	arg1	system					323:328	a ternary system	313:328	a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL)	313:407	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	6	43	theme	fibroblast	907:916	arg1	cells					918:922	NIH 3T3 mouse fibroblast cells	893:922	NIH 3T3 mouse fibroblast cells	893:922	In addition, the cytotoxicity of the films was evaluated by MTT assay against NIH 3T3 mouse fibroblast cells.					
31518954	2	44	theme	PVA	333:335	arg1	films					337:341	PVA films	333:341	PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL)	333:407	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	1	45	theme	polyvinyl	236:244	arg1	PVA					255:257	PVA	255:257	PVA	255:257	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	1	45	theme	polyvinyl	236:244	arg1	alcohol					246:252	polyvinyl alcohol	236:252	the polyvinyl alcohol (PVA) based films	232:270	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	0	46	with	fibers	58:63	arg1	properties					125:134	improved mechanical and antibacterial properties	87:134	improved mechanical and antibacterial properties	87:134	Reusable ternary PVA films containing bacterial cellulose fibers and ε-polylysine with improved mechanical and antibacterial properties.					
31518954	3	47	dep	aureus	548:553	arg1	bacteria					576:583	bacteria	576:583	bacteria	576:583	The prepared composite films showed more than 99% antibacterial properties against both Staphylococcus aureus and Escherichia coli bacteria.					
31518954	1	48	theme	alcohol	246:252	arg1	films					266:270	the polyvinyl alcohol (PVA) based films	232:270	the polyvinyl alcohol (PVA) based films	232:270	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	3	49	theme	prepared	449:456	arg1	films					468:472	The prepared composite films	445:472	The prepared composite films	445:472	The prepared composite films showed more than 99% antibacterial properties against both Staphylococcus aureus and Escherichia coli bacteria.					
31518954	1	50	theme	high	156:159	arg1	properties					172:181	high mechanical properties	156:181	high mechanical properties	156:181	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
31518954	5	51	theme	higher	755:760	arg1	properties					773:782	higher mechanical properties	755:782	higher mechanical properties	755:782	The films showed thermal stability and higher mechanical properties as compared to pure PVA films.					
31518954	6	52	theme	mouse	901:905	arg1	cells					918:922	NIH 3T3 mouse fibroblast cells	893:922	NIH 3T3 mouse fibroblast cells	893:922	In addition, the cytotoxicity of the films was evaluated by MTT assay against NIH 3T3 mouse fibroblast cells.					
31518954	2	53	theme	green	414:418	arg1	method					437:442	a green solution casting method	412:442	a green solution casting method	412:442	This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method.					
31518954	1	54	theme	mechanical	161:170	arg1	properties					172:181	high mechanical properties	156:181	high mechanical properties	156:181	The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging.					
29520318	8	0	theme	receptor	1235:1242	arg1	association					1244:1254	receptor association	1235:1254	receptor association	1235:1254	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	10	1	theme	influenza	1623:1631	arg1	virions					1633:1639	influenza virions	1623:1639	influenza virions	1623:1639	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	1	2	theme	cell	163:166	arg1	surface					168:174	the cell surface	159:174	the cell surface	159:174	Influenza virus infects cells by binding to sialylated glycans on the cell surface.					
29520318	8	3	theme	phase	1303:1307	arg1	separation					1309:1318	phase separation	1303:1318	phase separation	1303:1318	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	4	4	theme	membrane	555:562	arg1	composition					529:539	the sterol composition	518:539	the sterol composition of the target membrane	518:562	Using single-virus fluorescence microscopy, we demonstrate that the sterol composition of the target membrane enhances viral binding avidity in a dose-dependent manner.					
29520318	6	5	theme	phase	884:888	arg1	separation					890:899	liquid-liquid phase separation	870:899	liquid-liquid phase separation in model systems	870:916	Surprisingly, the ability of sterols to promote viral binding is independent of their ability to support liquid-liquid phase separation in model systems.					
29520318	5	6	theme	cooperative	639:649	arg1	dependence					651:660	a cooperative dependence	637:660	a cooperative dependence on concentration of receptors for influenza virus	637:710	Binding shows a cooperative dependence on concentration of receptors for influenza virus, as would be expected for a multivalent interaction.					
29520318	5	7	from	dependence	651:660	arg1	concentration					665:677	concentration	665:677	concentration of receptors for influenza virus	665:710	Binding shows a cooperative dependence on concentration of receptors for influenza virus, as would be expected for a multivalent interaction.					
29520318	10	8	theme	binding	1604:1610	arg1	avidity					1612:1618	the binding avidity	1600:1618	the binding avidity of influenza virions	1600:1639	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	4	9	theme	target	548:553	arg1	membrane					555:562	the target membrane	544:562	the target membrane	544:562	Using single-virus fluorescence microscopy, we demonstrate that the sterol composition of the target membrane enhances viral binding avidity in a dose-dependent manner.					
29520318	10	10	theme	finding	1510:1516	arg1	consequence					1490:1500	A natural consequence	1480:1500	A natural consequence of this finding	1480:1516	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	9	11	theme	membrane	1411:1418	arg1	receptors					1420:1428	membrane receptors	1411:1428	membrane receptors	1411:1428	This model explains how cholesterol and other sterols control the spatial organization of membrane receptors for influenza and increase viral binding avidity.					
29520318	8	12	theme	association	1244:1254	arg1	penalty					1224:1230	the entropic penalty	1211:1230	the entropic penalty of receptor association	1211:1254	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	9	13	theme	receptors	1420:1428	arg1	organization					1395:1406	the spatial organization	1383:1406	the spatial organization of membrane receptors for influenza	1383:1442	This model explains how cholesterol and other sterols control the spatial organization of membrane receptors for influenza and increase viral binding avidity.					
29520318	6	14	theme	ability	851:857	arg1	independent					830:840	independent	830:840	independent	830:840	Surprisingly, the ability of sterols to promote viral binding is independent of their ability to support liquid-liquid phase separation in model systems.					
29520318	6	14	theme	ability	851:857	arg1	ability					783:789	the ability	779:789	the ability of sterols to promote viral binding	779:825	Surprisingly, the ability of sterols to promote viral binding is independent of their ability to support liquid-liquid phase separation in model systems.					
29520318	10	15	from	concentration	1544:1556	arg1	membrane					1572:1579	the plasma membrane	1561:1579	the plasma membrane of cells	1561:1588	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	8	16	theme	entropic	1215:1222	arg1	penalty					1224:1230	the entropic penalty	1211:1230	the entropic penalty of receptor association	1211:1254	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	11	17	theme	cell	1808:1811	arg1	heterogeneity					1822:1834	cell membrane heterogeneity	1808:1834	cell membrane heterogeneity	1808:1834	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	5	18	theme	receptors	682:690	arg1	concentration					665:677	concentration	665:677	concentration of receptors for influenza virus	665:710	Binding shows a cooperative dependence on concentration of receptors for influenza virus, as would be expected for a multivalent interaction.					
29520318	1	19	theme	Influenza	93:101	arg1	virus					103:107	Influenza virus	93:107	Influenza virus	93:107	Influenza virus infects cells by binding to sialylated glycans on the cell surface.					
29520318	3	20	theme	spatial	406:412	arg1	organization					414:425	membrane spatial organization	397:425	membrane spatial organization	397:425	Here, we show that membrane spatial organization can control viral binding.					
29520318	4	21	theme	sterol	522:527	arg1	composition					529:539	the sterol composition	518:539	the sterol composition of the target membrane	518:562	Using single-virus fluorescence microscopy, we demonstrate that the sterol composition of the target membrane enhances viral binding avidity in a dose-dependent manner.					
29520318	10	22	theme	cells	1584:1588	arg1	membrane					1572:1579	the plasma membrane	1561:1579	the plasma membrane of cells	1561:1588	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	1	23	from	glycans	148:154	arg1	surface					168:174	the cell surface	159:174	the cell surface	159:174	Influenza virus infects cells by binding to sialylated glycans on the cell surface.					
29520318	11	24	theme	organization	1720:1731	arg1	form					1681:1684	a form	1679:1684	a form of cholesterol-dependent membrane organization that does not involve lipid rafts	1679:1765	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	8	25	theme	multimeric	1274:1283	arg1	complexes					1285:1293	multimeric complexes	1274:1293	multimeric complexes	1274:1293	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	5	26	theme	influenza	696:704	arg1	virus					706:710	influenza virus	696:710	influenza virus	696:710	Binding shows a cooperative dependence on concentration of receptors for influenza virus, as would be expected for a multivalent interaction.					
29520318	7	27	theme	molecular	979:987	arg1	simulations					998:1008	molecular dynamics simulations	979:1008	molecular dynamics simulations	979:1008	We develop a molecular explanation for this observation via molecular dynamics simulations, where we find that cholesterol promotes small-scale clusters of glycosphingolipid receptors.					
29520318	9	28	theme	other	1361:1365	arg1	sterols					1367:1373	other sterols	1361:1373	other sterols	1361:1373	This model explains how cholesterol and other sterols control the spatial organization of membrane receptors for influenza and increase viral binding avidity.					
29520318	10	29	theme	plasma	1565:1570	arg1	membrane					1572:1579	the plasma membrane	1561:1579	the plasma membrane of cells	1561:1588	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	0	30	theme	binding	31:37	arg1	avidity					39:45	influenza binding avidity	21:45	influenza binding avidity	21:45	Cholesterol enhances influenza binding avidity by controlling nanoscale receptor clustering.					
29520318	9	31	theme	viral	1457:1461	arg1	avidity					1471:1477	viral binding avidity	1457:1477	viral binding avidity	1457:1477	This model explains how cholesterol and other sterols control the spatial organization of membrane receptors for influenza and increase viral binding avidity.					
29520318	5	32	theme	multivalent	740:750	arg1	interaction					752:762	a multivalent interaction	738:762	a multivalent interaction	738:762	Binding shows a cooperative dependence on concentration of receptors for influenza virus, as would be expected for a multivalent interaction.					
29520318	7	33	theme	dynamics	989:996	arg1	simulations					998:1008	molecular dynamics simulations	979:1008	molecular dynamics simulations	979:1008	We develop a molecular explanation for this observation via molecular dynamics simulations, where we find that cholesterol promotes small-scale clusters of glycosphingolipid receptors.					
29520318	3	34	theme	membrane	397:404	arg1	organization					414:425	membrane spatial organization	397:425	membrane spatial organization	397:425	Here, we show that membrane spatial organization can control viral binding.					
29520318	9	35	theme	binding	1463:1469	arg1	avidity					1471:1477	viral binding avidity	1457:1477	viral binding avidity	1457:1477	This model explains how cholesterol and other sterols control the spatial organization of membrane receptors for influenza and increase viral binding avidity.					
29520318	8	36	theme	monomeric	1154:1162	arg1	state					1164:1168	the monomeric state	1150:1168	the monomeric state of glycosphingolipid receptors	1150:1199	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	2	37	theme	chemical	187:194	arg1	structure					196:204	the chemical structure	183:204	the chemical structure of these glycans	183:221	While the chemical structure of these glycans determines hemagglutinin-glycan binding affinity, bimolecular affinities are weak, so binding is avidity-dominated and driven by multivalent interactions.					
29520318	10	38	theme	cholesterol	1532:1542	arg1	concentration					1544:1556	local cholesterol concentration	1526:1556	local cholesterol concentration in the plasma membrane of cells	1526:1588	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	3	39	theme	viral	439:443	arg1	binding					445:451	viral binding	439:451	viral binding	439:451	Here, we show that membrane spatial organization can control viral binding.					
29520318	4	40	theme	dose-dependent	600:613	arg1	manner					615:620	a dose-dependent manner	598:620	a dose-dependent manner	598:620	Using single-virus fluorescence microscopy, we demonstrate that the sterol composition of the target membrane enhances viral binding avidity in a dose-dependent manner.					
29520318	8	41	theme	glycosphingolipid	1173:1189	arg1	receptors					1191:1199	glycosphingolipid receptors	1173:1199	glycosphingolipid receptors	1173:1199	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	11	42	theme	lipid	1755:1759	arg1	rafts					1761:1765	lipid rafts	1755:1765	lipid rafts	1755:1765	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	4	43	theme	viral	573:577	arg1	avidity					587:593	viral binding avidity	573:593	viral binding avidity	573:593	Using single-virus fluorescence microscopy, we demonstrate that the sterol composition of the target membrane enhances viral binding avidity in a dose-dependent manner.					
29520318	1	44	gly	sialylated	137:146	arg1	glycans					148:154	sialylated glycans	137:154	sialylated glycans on the cell surface	137:174	Influenza virus infects cells by binding to sialylated glycans on the cell surface.					
29520318	11	45	theme	membrane	1813:1820	arg1	heterogeneity					1822:1834	cell membrane heterogeneity	1808:1834	cell membrane heterogeneity	1808:1834	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	2	46	theme	binding	255:261	arg1	affinity					263:270	hemagglutinin-glycan binding affinity	234:270	hemagglutinin-glycan binding affinity	234:270	While the chemical structure of these glycans determines hemagglutinin-glycan binding affinity, bimolecular affinities are weak, so binding is avidity-dominated and driven by multivalent interactions.					
29520318	6	47	theme	viral	813:817	arg1	binding					819:825	viral binding	813:825	viral binding	813:825	Surprisingly, the ability of sterols to promote viral binding is independent of their ability to support liquid-liquid phase separation in model systems.					
29520318	0	48	theme	nanoscale	62:70	arg1	clustering					81:90	nanoscale receptor clustering	62:90	nanoscale receptor clustering	62:90	Cholesterol enhances influenza binding avidity by controlling nanoscale receptor clustering.					
29520318	6	49	theme	model	904:908	arg1	systems					910:916	model systems	904:916	model systems	904:916	Surprisingly, the ability of sterols to promote viral binding is independent of their ability to support liquid-liquid phase separation in model systems.					
29520318	2	50	theme	hemagglutinin-glycan	234:253	arg1	affinity					263:270	hemagglutinin-glycan binding affinity	234:270	hemagglutinin-glycan binding affinity	234:270	While the chemical structure of these glycans determines hemagglutinin-glycan binding affinity, bimolecular affinities are weak, so binding is avidity-dominated and driven by multivalent interactions.					
29520318	11	51	theme	different	1871:1879	arg1	factors					1881:1887	several different factors	1863:1887	several different factors	1863:1887	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	6	52	from	separation	890:899	arg1	systems					910:916	model systems	904:916	model systems	904:916	Surprisingly, the ability of sterols to promote viral binding is independent of their ability to support liquid-liquid phase separation in model systems.					
29520318	8	53	theme	receptors	1191:1199	arg1	state					1164:1168	the monomeric state	1150:1168	the monomeric state of glycosphingolipid receptors	1150:1199	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	2	54	theme	glycans	215:221	arg1	structure					196:204	the chemical structure	183:204	the chemical structure of these glycans	183:221	While the chemical structure of these glycans determines hemagglutinin-glycan binding affinity, bimolecular affinities are weak, so binding is avidity-dominated and driven by multivalent interactions.					
29520318	10	55	theme	local	1526:1530	arg1	concentration					1544:1556	local cholesterol concentration	1526:1556	local cholesterol concentration in the plasma membrane of cells	1526:1588	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	11	56	theme	membrane	1711:1718	arg1	organization					1720:1731	cholesterol-dependent membrane organization	1689:1731	cholesterol-dependent membrane organization	1689:1731	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	11	57	from	effect	1798:1803	arg1	heterogeneity					1822:1834	cell membrane heterogeneity	1808:1834	cell membrane heterogeneity	1808:1834	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	11	58	theme	cholesterol-dependent	1689:1709	arg1	organization					1720:1731	cholesterol-dependent membrane organization	1689:1731	cholesterol-dependent membrane organization	1689:1731	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	4	59	theme	binding	579:585	arg1	avidity					587:593	viral binding avidity	573:593	viral binding avidity	573:593	Using single-virus fluorescence microscopy, we demonstrate that the sterol composition of the target membrane enhances viral binding avidity in a dose-dependent manner.					
29520318	10	60	theme	virions	1633:1639	arg1	avidity					1612:1618	the binding avidity	1600:1618	the binding avidity of influenza virions	1600:1639	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	11	61	theme	several	1863:1869	arg1	factors					1881:1887	several different factors	1863:1887	several different factors	1863:1887	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	6	62	theme	sterols	794:800	arg1	ability					783:789	the ability	779:789	the ability of sterols to promote viral binding	779:825	Surprisingly, the ability of sterols to promote viral binding is independent of their ability to support liquid-liquid phase separation in model systems.					
29520318	6	62	theme	sterols	794:800	arg1	independent					830:840	independent	830:840	independent	830:840	Surprisingly, the ability of sterols to promote viral binding is independent of their ability to support liquid-liquid phase separation in model systems.					
29520318	1	63	theme	sialylated	137:146	arg1	glycans					148:154	sialylated glycans	137:154	sialylated glycans on the cell surface	137:174	Influenza virus infects cells by binding to sialylated glycans on the cell surface.					
29520318	8	64	dep	orders	1143:1148	arg1	reducing					1202:1209	reducing	1202:1209	reducing the entropic penalty of receptor association	1202:1254	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	8	64	dep	orders	1143:1148	arg1	favoring					1265:1272	favoring	1265:1272	thus favoring multimeric complexes without phase separation	1260:1318	We propose a model whereby cholesterol orders the monomeric state of glycosphingolipid receptors, reducing the entropic penalty of receptor association and thus favoring multimeric complexes without phase separation.					
29520318	7	65	theme	glycosphingolipid	1075:1091	arg1	receptors					1093:1101	glycosphingolipid receptors	1075:1101	glycosphingolipid receptors	1075:1101	We develop a molecular explanation for this observation via molecular dynamics simulations, where we find that cholesterol promotes small-scale clusters of glycosphingolipid receptors.					
29520318	4	66	theme	fluorescence	473:484	arg1	microscopy					486:495	single-virus fluorescence microscopy	460:495	single-virus fluorescence microscopy	460:495	Using single-virus fluorescence microscopy, we demonstrate that the sterol composition of the target membrane enhances viral binding avidity in a dose-dependent manner.					
29520318	7	67	theme	receptors	1093:1101	arg1	clusters					1063:1070	small-scale clusters	1051:1070	small-scale clusters of glycosphingolipid receptors	1051:1101	We develop a molecular explanation for this observation via molecular dynamics simulations, where we find that cholesterol promotes small-scale clusters of glycosphingolipid receptors.					
29520318	7	68	theme	small-scale	1051:1061	arg1	clusters					1063:1070	small-scale clusters	1051:1070	small-scale clusters of glycosphingolipid receptors	1051:1101	We develop a molecular explanation for this observation via molecular dynamics simulations, where we find that cholesterol promotes small-scale clusters of glycosphingolipid receptors.					
29520318	2	69	theme	multivalent	352:362	arg1	interactions					364:375	multivalent interactions	352:375	multivalent interactions	352:375	While the chemical structure of these glycans determines hemagglutinin-glycan binding affinity, bimolecular affinities are weak, so binding is avidity-dominated and driven by multivalent interactions.					
29520318	4	70	theme	single-virus	460:471	arg1	microscopy					486:495	single-virus fluorescence microscopy	460:495	single-virus fluorescence microscopy	460:495	Using single-virus fluorescence microscopy, we demonstrate that the sterol composition of the target membrane enhances viral binding avidity in a dose-dependent manner.					
29520318	2	71	theme	bimolecular	273:283	arg1	affinities					285:294	bimolecular affinities	273:294	bimolecular affinities	273:294	While the chemical structure of these glycans determines hemagglutinin-glycan binding affinity, bimolecular affinities are weak, so binding is avidity-dominated and driven by multivalent interactions.					
29520318	10	72	theme	natural	1482:1488	arg1	consequence					1490:1500	A natural consequence	1480:1500	A natural consequence of this finding	1480:1516	A natural consequence of this finding is that local cholesterol concentration in the plasma membrane of cells may alter the binding avidity of influenza virions.					
29520318	6	73	theme	liquid-liquid	870:882	arg1	separation					890:899	liquid-liquid phase separation	870:899	liquid-liquid phase separation in model systems	870:916	Surprisingly, the ability of sterols to promote viral binding is independent of their ability to support liquid-liquid phase separation in model systems.					
29520318	0	74	theme	receptor	72:79	arg1	clustering					81:90	nanoscale receptor clustering	62:90	nanoscale receptor clustering	62:90	Cholesterol enhances influenza binding avidity by controlling nanoscale receptor clustering.					
29520318	9	75	theme	spatial	1387:1393	arg1	organization					1395:1406	the spatial organization	1383:1406	the spatial organization of membrane receptors for influenza	1383:1442	This model explains how cholesterol and other sterols control the spatial organization of membrane receptors for influenza and increase viral binding avidity.					
29520318	0	76	theme	influenza	21:29	arg1	avidity					39:45	influenza binding avidity	21:45	influenza binding avidity	21:45	Cholesterol enhances influenza binding avidity by controlling nanoscale receptor clustering.					
29520318	7	77	theme	molecular	932:940	arg1	explanation					942:952	a molecular explanation	930:952	a molecular explanation for this observation	930:973	We develop a molecular explanation for this observation via molecular dynamics simulations, where we find that cholesterol promotes small-scale clusters of glycosphingolipid receptors.					
29520318	11	78	theme	factors	1881:1887	arg1	interplay					1850:1858	the interplay	1846:1858	the interplay of several different factors	1846:1887	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
29520318	11	78	theme	factors	1881:1887	arg1	effect					1798:1803	cholesterol's effect	1784:1803	cholesterol's effect on cell membrane heterogeneity	1784:1834	Furthermore, our results demonstrate a form of cholesterol-dependent membrane organization that does not involve lipid rafts, suggesting that cholesterol's effect on cell membrane heterogeneity is likely the interplay of several different factors.					
30476771	3	0	theme	66.25 mg/g	636:645	arg1	Cr					625:626	Cr(III)	625:631	Cr(III) of 66.25 mg/g (150 min)	625:655	The CLCh/MWCNT/Fe film presented a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min) at 25 °C.					
30476771	4	1	theme	aqueous	755:761	arg1	solution					763:770	the aqueous solution	751:770	the aqueous solution	751:770	The CLCh/MWCNT/Fe can be easily removed from the aqueous solution by a mechanical separation or by magnetization due to its magnetic properties.					
30476771	3	2	dep	Cr	665:666	arg1	VI					668:669	VI	668:669	VI	668:669	The CLCh/MWCNT/Fe film presented a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min) at 25 °C.					
30476771	2	3	theme	Raman	538:542	arg1	Spectroscopy					544:555	Raman Spectroscopy	538:555	Raman Spectroscopy	538:555	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	1	4	link	crosslinked	248:258	arg1	CLCh					270:273	CLCh	270:273	CLCh	270:273	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	4	link	crosslinked	248:258	arg1	chitosan					260:267	crosslinked chitosan	248:267	crosslinked chitosan (CLCh)	248:274	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	2	5	theme	Mass	343:346	arg1	Spectrometry					348:359	Inductively Coupled Plasma Mass Spectrometry	316:359	Inductively Coupled Plasma Mass Spectrometry (ICP-MS)	316:368	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	5	theme	Mass	343:346	arg1	ICP-MS					362:367	ICP-MS	362:367	ICP-MS	362:367	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	4	6	attach	removed	738:744	arg2	CLCh/MWCNT/Fe					710:722	The CLCh/MWCNT/Fe	706:722	The CLCh/MWCNT/Fe	706:722	The CLCh/MWCNT/Fe can be easily removed from the aqueous solution by a mechanical separation or by magnetization due to its magnetic properties.					
30476771	4	6	attach	removed	738:744	arg1	solution					763:770	the aqueous solution	751:770	the aqueous solution	751:770	The CLCh/MWCNT/Fe can be easily removed from the aqueous solution by a mechanical separation or by magnetization due to its magnetic properties.					
30476771	5	7	dep	%	963:963	arg1	VI					1006:1007	VI	1006:1007	VI	1006:1007	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	4	8	theme	magnetic	830:837	arg1	properties					839:848	its magnetic properties	826:848	its magnetic properties	826:848	The CLCh/MWCNT/Fe can be easily removed from the aqueous solution by a mechanical separation or by magnetization due to its magnetic properties.					
30476771	1	9	theme	crosslinked	248:258	arg1	CLCh					270:273	CLCh	270:273	CLCh	270:273	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	9	theme	crosslinked	248:258	arg1	chitosan					260:267	crosslinked chitosan	248:267	crosslinked chitosan (CLCh)	248:274	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	2	10	theme	Dispersive	476:485	arg1	EDS					501:503	EDS	501:503	EDS	501:503	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	10	theme	Dispersive	476:485	arg1	Spectrometry					487:498	Energy Dispersive Spectrometry	469:498	Energy Dispersive Spectrometry (EDS)	469:504	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	11	theme	Energy	469:474	arg1	EDS					501:503	EDS	501:503	EDS	501:503	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	11	theme	Energy	469:474	arg1	Spectrometry					487:498	Energy Dispersive Spectrometry	469:498	Energy Dispersive Spectrometry (EDS)	469:504	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	5	12	theme	consecutive	858:868	arg1	cycles					895:900	ten consecutive reutilization adsorption cycles	854:900	ten consecutive reutilization adsorption cycles	854:900	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	1	13	theme	walled	168:173	arg1	nanotubes					182:190	multiple walled carbon nanotubes	159:190	multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh)	159:274	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	13	theme	walled	168:173	arg1	MWCNT					193:197	MWCNT	193:197	MWCNT	193:197	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	5	14	theme	reutilization	870:882	arg1	cycles					895:900	ten consecutive reutilization adsorption cycles	854:900	ten consecutive reutilization adsorption cycles	854:900	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	1	15	theme	carbon	175:180	arg1	nanotubes					182:190	multiple walled carbon nanotubes	159:190	multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh)	159:274	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	15	theme	carbon	175:180	arg1	MWCNT					193:197	MWCNT	193:197	MWCNT	193:197	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	4	16	theme	due	819:821	arg1	magnetization					805:817	magnetization	805:817	magnetization due to its magnetic properties	805:848	The CLCh/MWCNT/Fe can be easily removed from the aqueous solution by a mechanical separation or by magnetization due to its magnetic properties.					
30476771	5	17	theme	Cr	991:992	arg1	removal					980:986	the removal	976:986	the removal of Cr(III) and Cr	976:1004	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	0	18	theme	Iron	0:3	arg1	oxide/carbon					5:16	Iron oxide/carbon	0:16	Iron oxide/carbon	0:16	Iron oxide/carbon nanotubes/chitosan magnetic composite film for chromium species removal.					
30476771	0	19	theme	magnetic	37:44	arg1	film					56:59	magnetic composite film	37:59	magnetic composite film for chromium species removal	37:88	Iron oxide/carbon nanotubes/chitosan magnetic composite film for chromium species removal.					
30476771	2	20	theme	X-ray	408:412	arg1	XRD					427:429	XRD	427:429	XRD	427:429	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	20	theme	X-ray	408:412	arg1	Diffraction					414:424	X-ray Diffraction	408:424	X-ray Diffraction (XRD)	408:430	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	3	21	theme	maximum	593:599	arg1	capacity					612:619	a maximum adsorption capacity	591:619	a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min)	591:694	The CLCh/MWCNT/Fe film presented a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min) at 25 °C.					
30476771	1	22	theme	multiple	159:166	arg1	nanotubes					182:190	multiple walled carbon nanotubes	159:190	multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh)	159:274	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	22	theme	multiple	159:166	arg1	MWCNT					193:197	MWCNT	193:197	MWCNT	193:197	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	2	23	theme	Electron	442:449	arg1	SEM					463:465	SEM	463:465	SEM	463:465	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	23	theme	Electron	442:449	arg1	Microscopy					451:460	Scanning Electron Microscopy	433:460	Scanning Electron Microscopy (SEM)	433:466	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	3	24	theme	adsorption	601:610	arg1	capacity					612:619	a maximum adsorption capacity	591:619	a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min)	591:694	The CLCh/MWCNT/Fe film presented a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min) at 25 °C.					
30476771	1	25	dep	nanotubes	182:190	arg1	doped					200:204	doped	200:204	doped with magnetic iron oxide	200:229	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	25	dep	nanotubes	182:190	arg1	deposited					235:243	deposited	235:243	deposited in crosslinked chitosan (CLCh)	235:274	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	0	26	theme	composite	46:54	arg1	film					56:59	magnetic composite film	37:59	magnetic composite film for chromium species removal	37:88	Iron oxide/carbon nanotubes/chitosan magnetic composite film for chromium species removal.					
30476771	3	27	theme	449.30 mg/g	675:685	arg1	Cr					665:666	Cr(VI) of 449.30 mg/g (60 min)	665:694	Cr(VI) of 449.30 mg/g (60 min)	665:694	The CLCh/MWCNT/Fe film presented a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min) at 25 °C.					
30476771	2	28	theme	Scanning	433:440	arg1	SEM					463:465	SEM	463:465	SEM	463:465	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	28	theme	Scanning	433:440	arg1	Microscopy					451:460	Scanning Electron Microscopy	433:460	Scanning Electron Microscopy (SEM)	433:466	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	5	29	theme	%	970:970	arg1	losses					946:951	efficiency losses	935:951	efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI)	935:1008	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	2	30	theme	N2	381:382	arg1	adsorption/desorption					385:405	nitrogen (N2) adsorption/desorption	371:405	nitrogen (N2) adsorption/desorption	371:405	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	0	31	theme	chromium	65:72	arg1	species					74:80	chromium species	65:80	chromium species removal	65:88	Iron oxide/carbon nanotubes/chitosan magnetic composite film for chromium species removal.					
30476771	5	32	theme	Cr	1003:1004	arg1	removal					980:986	the removal	976:986	the removal of Cr(III) and Cr	976:1004	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	4	33	theme	mechanical	777:786	arg1	separation					788:797	a mechanical separation	775:797	a mechanical separation	775:797	The CLCh/MWCNT/Fe can be easily removed from the aqueous solution by a mechanical separation or by magnetization due to its magnetic properties.					
30476771	5	34	theme	efficiency	935:944	arg1	losses					946:951	efficiency losses	935:951	efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI)	935:1008	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	2	35	theme	Plasma	336:341	arg1	Spectrometry					348:359	Inductively Coupled Plasma Mass Spectrometry	316:359	Inductively Coupled Plasma Mass Spectrometry (ICP-MS)	316:368	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	35	theme	Plasma	336:341	arg1	ICP-MS					362:367	ICP-MS	362:367	ICP-MS	362:367	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	3	36	attach	presented	581:589	arg2	film					576:579	The CLCh/MWCNT/Fe film	558:579	The CLCh/MWCNT/Fe film	558:579	The CLCh/MWCNT/Fe film presented a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min) at 25 °C.					
30476771	3	36	attach	presented	581:589	arg1	25 °C					699:703	25 °C	699:703	25 °C	699:703	The CLCh/MWCNT/Fe film presented a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min) at 25 °C.					
30476771	2	37	theme	Coupled	328:334	arg1	Spectrometry					348:359	Inductively Coupled Plasma Mass Spectrometry	316:359	Inductively Coupled Plasma Mass Spectrometry (ICP-MS)	316:368	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	37	theme	Coupled	328:334	arg1	ICP-MS					362:367	ICP-MS	362:367	ICP-MS	362:367	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	5	38	theme	adsorption	884:893	arg1	cycles					895:900	ten consecutive reutilization adsorption cycles	854:900	ten consecutive reutilization adsorption cycles	854:900	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	3	39	theme	CLCh/MWCNT/Fe	562:574	arg1	film					576:579	The CLCh/MWCNT/Fe film	558:579	The CLCh/MWCNT/Fe film	558:579	The CLCh/MWCNT/Fe film presented a maximum adsorption capacity for Cr(III) of 66.25 mg/g (150 min) and for Cr(VI) of 449.30 mg/g (60 min) at 25 °C.					
30476771	1	40	theme	CLCh/MWCNT/Fe	121:133	arg1	film					135:138	a CLCh/MWCNT/Fe film	119:138	a CLCh/MWCNT/Fe film	119:138	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	41	from	form	111:114	arg1	adsorbent					94:102	adsorbent	94:102	adsorbent	94:102	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	42	theme	magnetic	211:218	arg1	oxide					225:229	magnetic iron oxide	211:229	magnetic iron oxide	211:229	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	2	43	theme	Infrared	507:514	arg1	Spectroscopy					516:527	Infrared Spectroscopy	507:527	Infrared Spectroscopy (IR)	507:532	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	2	43	theme	Infrared	507:514	arg1	IR					530:531	IR	530:531	IR	530:531	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	1	44	theme	film	135:138	arg1	form					111:114	the form	107:114	the form of a CLCh/MWCNT/Fe film	107:138	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	45	from	adsorbent	94:102	arg1	form					111:114	the form	107:114	the form of a CLCh/MWCNT/Fe film	107:138	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	1	46	theme	iron	220:223	arg1	oxide					225:229	magnetic iron oxide	211:229	magnetic iron oxide	211:229	An adsorbent in the form of a CLCh/MWCNT/Fe film was prepared using multiple walled carbon nanotubes (MWCNT) doped with magnetic iron oxide and deposited in crosslinked chitosan (CLCh).					
30476771	5	47	theme	%	963:963	arg1	losses					946:951	efficiency losses	935:951	efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI)	935:1008	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	2	48	theme	nitrogen	371:378	arg1	adsorption/desorption					385:405	nitrogen (N2) adsorption/desorption	371:405	nitrogen (N2) adsorption/desorption	371:405	The CLCh/MWCNT/Fe was characterized by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), nitrogen (N2) adsorption/desorption, X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectrometry (EDS), Infrared Spectroscopy (IR) and Raman Spectroscopy.					
30476771	0	49	theme	species	74:80	arg1	removal					82:88	chromium species removal	65:88	chromium species removal	65:88	Iron oxide/carbon nanotubes/chitosan magnetic composite film for chromium species removal.					
30476771	5	50	theme	CLCh/MWCNT/Fe	906:918	arg1	film					920:923	the CLCh/MWCNT/Fe film	902:923	the CLCh/MWCNT/Fe film	902:923	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	5	51	attach	presented	925:933	arg2	film					920:923	the CLCh/MWCNT/Fe film	902:923	the CLCh/MWCNT/Fe film	902:923	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30476771	5	51	attach	presented	925:933	arg1	cycles					895:900	ten consecutive reutilization adsorption cycles	854:900	ten consecutive reutilization adsorption cycles	854:900	In ten consecutive reutilization adsorption cycles the CLCh/MWCNT/Fe film presented efficiency losses of only 12% and 6% for the removal of Cr(III) and Cr(VI), respectively.					
30584938	6	0	theme	23.06 ± 0.19 MPa	933:948	arg1	maximum					922:928	maximum	922:928	maximum of 23.06 ± 0.19 MPa	922:948	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	1	1	from	bio-sources	237:247	arg1	development					173:183	development	173:183	development of active films from non-toxic and antioxidant rich bio-sources	173:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	1	1	from	bio-sources	237:247	arg1	films					195:199	active films	188:199	active films from non-toxic and antioxidant rich bio-sources	188:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	7	2	theme	radical	1145:1151	arg1	scavenging					1153:1162	ABTS radical scavenging	1140:1162	ABTS radical scavenging	1140:1162	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	3	3	theme	optical	488:494	arg1	nature					496:501	optical nature	488:501	optical nature	488:501	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	5	4	theme	reduced	789:795	arg1	penetrability					828:840	the reduced hydrophilicity and water vapor penetrability	785:840	the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion	785:874	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	4	5	theme	increased	656:664	arg1	thickness					666:674	increased thickness	656:674	increased thickness	656:674	Increase in the MLE concentration resulted in films with increased thickness and decreased moisture content.					
30584938	10	6	theme	promising	1603:1611	arg1	nature					1613:1618	the potential and promising nature	1585:1618	the potential and promising nature of MLE impregnated chitosan films	1585:1652	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	7	7	theme	reducing	1121:1128	arg1	power					1130:1134	ferric reducing power	1114:1134	ferric reducing power	1114:1134	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	7	8	theme	incremental	1210:1220	arg1	MLE					1233:1235	MLE	1233:1235	MLE in the chitosan films	1233:1257	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	7	8	theme	incremental	1210:1220	arg1	amounts					1222:1228	the incremental amounts	1206:1228	the incremental amounts of MLE in the chitosan films	1206:1257	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	9	9	theme	oxidation	1479:1487	arg1	resistance					1489:1498	56% higher oxidation resistance	1468:1498	56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film	1468:1564	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	6	10	theme	better	897:902	arg1	strength					912:919	better tensile strength	897:919	better tensile strength	897:919	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	10	11	theme	films	1648:1652	arg1	nature					1613:1618	the potential and promising nature	1585:1618	the potential and promising nature of MLE impregnated chitosan films	1585:1652	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	3	12	theme	composite	570:578	arg1	films					580:584	the chitosan-MLE composite films	553:584	the chitosan-MLE composite films	553:584	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	1	13	theme	active	188:193	arg1	films					195:199	active films	188:199	active films from non-toxic and antioxidant rich bio-sources	188:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	6	14	theme	pure	990:993	arg1	18.14 ± 0.72 MPa					1010:1025	18.14 ± 0.72 MPa	1010:1025	18.14 ± 0.72 MPa	1010:1025	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	6	14	theme	pure	990:993	arg1	film					1004:1007	the pure chitosan film	986:1007	the pure chitosan film (18.14 ± 0.72 MPa)	986:1026	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	10	15	theme	impregnated	1627:1637	arg1	films					1648:1652	MLE impregnated chitosan films	1623:1652	MLE impregnated chitosan films	1623:1652	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	5	16	theme	angle	716:720	arg1	analysis					763:770	Contact angle, water solubility and vapor permeability analysis	708:770	Contact angle, water solubility and vapor permeability analysis	708:770	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	9	17	theme	5	1508:1508	arg1	%					1509:1509	%	1509:1509	%	1509:1509	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	10	18	theme	packaging	1689:1697	arg1	films					1699:1703	active packaging films	1682:1703	active packaging films for food preservation	1682:1725	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	7	19	from	assessment	1042:1051	arg1	terms					1056:1060	terms	1056:1060	terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging	1056:1162	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	5	20	theme	water	723:727	arg1	solubility					729:738	water solubility	723:738	water solubility	723:738	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	9	21	theme	MLE	1511:1513	arg1	film					1515:1518	the 5% MLE film	1504:1518	the 5% MLE film than a commercial polyamide/polyethylene film	1504:1564	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	6	22	theme	elongation	964:973	arg1	ratio					975:979	reduced elongation ratio	956:979	reduced elongation ratio	956:979	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	10	23	theme	suitable	1657:1664	arg1	alternative					1666:1676	suitable alternative	1657:1676	suitable alternative for active packaging films for food preservation	1657:1725	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	7	24	theme	phenolic	1071:1078	arg1	content					1080:1086	total phenolic content	1065:1086	total phenolic content	1065:1086	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	3	25	theme	mechanical	523:532	arg1	characteristics					534:548	mechanical characteristics	523:548	mechanical characteristics	523:548	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	1	26	theme	films	133:137	arg1	usage					116:120	usage	116:120	usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources	116:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	1	27	theme	plastic	125:131	arg1	films					133:137	plastic films	125:137	plastic films	125:137	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	4	28	theme	moisture	690:697	arg1	content					699:705	decreased moisture content	680:705	decreased moisture content	680:705	Increase in the MLE concentration resulted in films with increased thickness and decreased moisture content.					
30584938	0	29	theme	active	62:67	arg1	packaging					74:82	active food packaging	62:82	active food packaging	62:82	Mango leaf extract incorporated chitosan antioxidant film for active food packaging.					
30584938	1	30	theme	food	143:146	arg1	demands					161:167	food preservation demands	143:167	food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources	143:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	8	31	theme	low	1362:1364	arg1	rates					1383:1387	low oxygen transport rates	1362:1387	low oxygen transport rates	1362:1387	Microscopic studies revealed the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates.					
30584938	4	32	from	Increase	599:606	arg1	concentration					619:631	the MLE concentration	611:631	the MLE concentration	611:631	Increase in the MLE concentration resulted in films with increased thickness and decreased moisture content.					
30584938	8	33	theme	transport	1373:1381	arg1	rates					1383:1387	low oxygen transport rates	1362:1387	low oxygen transport rates	1362:1387	Microscopic studies revealed the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates.					
30584938	9	34	theme	commercial	1527:1536	arg1	film					1561:1564	a commercial polyamide/polyethylene film	1525:1564	a commercial polyamide/polyethylene film	1525:1564	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	3	35	theme	MLE	448:450	arg1	variation					452:460	MLE variation	448:460	MLE variation (1-5%)	448:467	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	3	35	theme	MLE	448:450	arg1	%					466:466	1-5%	463:466	1-5%	463:466	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	5	36	theme	permeability	750:761	arg1	analysis					763:770	Contact angle, water solubility and vapor permeability analysis	708:770	Contact angle, water solubility and vapor permeability analysis	708:770	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	5	37	theme	hydrophilicity	797:810	arg1	penetrability					828:840	the reduced hydrophilicity and water vapor penetrability	785:840	the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion	785:874	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	9	38	dep	studies	1402:1408	arg1	cashew					1413:1418	cashew	1413:1418	to cashew nuts preservation for 28 days storage	1410:1456	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	2	39	theme	leaf	404:407	arg1	extract					409:415	mango leaf extract	398:415	mango leaf extract (MLE) incorporation	398:435	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	2	39	theme	leaf	404:407	arg1	MLE					418:420	MLE	418:420	MLE	418:420	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	0	40	theme	Mango	0:4	arg1	extract					11:17	Mango leaf extract	0:17	Mango leaf extract	0:17	Mango leaf extract incorporated chitosan antioxidant film for active food packaging.					
30584938	5	41	theme	films	849:853	arg1	penetrability					828:840	the reduced hydrophilicity and water vapor penetrability	785:840	the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion	785:874	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	8	42	theme	films	1346:1350	arg1	nature					1319:1324	the smooth, compact and dense nature	1289:1324	the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates	1289:1387	Microscopic studies revealed the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates.					
30584938	7	43	theme	antioxidant	1180:1190	arg1	activity					1192:1199	improved antioxidant activity	1171:1199	improved antioxidant activity	1171:1199	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	9	44	theme	Application	1390:1400	arg1	studies					1402:1408	Application studies	1390:1408	Application studies to cashew nuts preservation for 28 days storage	1390:1456	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	0	45	theme	chitosan	32:39	arg1	film					53:56	chitosan antioxidant film	32:56	chitosan antioxidant film for active food packaging	32:82	Mango leaf extract incorporated chitosan antioxidant film for active food packaging.					
30584938	6	46	theme	MLE	877:879	arg1	films					881:885	MLE films	877:885	MLE films	877:885	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	5	47	theme	vapor	822:826	arg1	penetrability					828:840	the reduced hydrophilicity and water vapor penetrability	785:840	the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion	785:874	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	2	48	theme	films	349:353	arg1	studies					329:335	the development, characterization and application studies	279:335	the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation	279:435	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	1	49	theme	antioxidant	220:230	arg1	bio-sources					237:247	non-toxic and antioxidant rich bio-sources	206:247	non-toxic and antioxidant rich bio-sources	206:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	8	50	theme	MLE-chitosan	1333:1344	arg1	films					1346:1350	the MLE-chitosan films	1329:1350	the MLE-chitosan films favouring low oxygen transport rates	1329:1387	Microscopic studies revealed the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates.					
30584938	5	51	theme	water	816:820	arg1	vapor					822:826	water vapor	816:826	water vapor	816:826	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	7	52	theme	ABTS	1140:1143	arg1	scavenging					1153:1162	ABTS radical scavenging	1140:1162	ABTS radical scavenging	1140:1162	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	2	53	theme	antioxidant	374:384	arg1	activity					386:393	their antioxidant activity	368:393	their antioxidant activity	368:393	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	10	54	theme	MLE	1623:1625	arg1	films					1648:1652	MLE impregnated chitosan films	1623:1652	MLE impregnated chitosan films	1623:1652	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	7	55	theme	power	1130:1134	arg1	terms					1056:1060	terms	1056:1060	terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging	1056:1162	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	2	56	theme	characterization	296:311	arg1	studies					329:335	the development, characterization and application studies	279:335	the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation	279:435	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	7	57	theme	ferric	1114:1119	arg1	power					1130:1134	ferric reducing power	1114:1134	ferric reducing power	1114:1134	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	2	58	theme	development	283:293	arg1	studies					329:335	the development, characterization and application studies	279:335	the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation	279:435	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	9	59	theme	higher	1472:1477	arg1	resistance					1489:1498	56% higher oxidation resistance	1468:1498	56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film	1468:1564	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	6	60	theme	tensile	904:910	arg1	strength					912:919	better tensile strength	897:919	better tensile strength	897:919	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	8	61	theme	Microscopic	1260:1270	arg1	studies					1272:1278	Microscopic studies	1260:1278	Microscopic studies	1260:1278	Microscopic studies revealed the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates.					
30584938	7	62	theme	chitosan	1244:1251	arg1	films					1253:1257	the chitosan films	1240:1257	the chitosan films	1240:1257	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	7	63	theme	scavenging	1102:1111	arg1	terms					1056:1060	terms	1056:1060	terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging	1056:1162	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	3	64	theme	chitosan-MLE	557:568	arg1	films					580:584	the chitosan-MLE composite films	553:584	the chitosan-MLE composite films	553:584	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	6	65	theme	chitosan	995:1002	arg1	18.14 ± 0.72 MPa					1010:1025	18.14 ± 0.72 MPa	1010:1025	18.14 ± 0.72 MPa	1010:1025	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	6	65	theme	chitosan	995:1002	arg1	film					1004:1007	the pure chitosan film	986:1007	the pure chitosan film (18.14 ± 0.72 MPa)	986:1026	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	10	66	theme	chitosan	1639:1646	arg1	films					1648:1652	MLE impregnated chitosan films	1623:1652	MLE impregnated chitosan films	1623:1652	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	5	67	theme	Contact	708:714	arg1	angle					716:720	Contact angle	708:720	Contact angle	708:720	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	2	68	theme	application	317:327	arg1	studies					329:335	the development, characterization and application studies	279:335	the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation	279:435	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	1	69	theme	films	195:199	arg1	development					173:183	development	173:183	development of active films from non-toxic and antioxidant rich bio-sources	173:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	7	70	from	amounts	1222:1228	arg1	films					1253:1257	the chitosan films	1240:1257	the chitosan films	1240:1257	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	8	71	theme	smooth	1293:1298	arg1	nature					1319:1324	the smooth, compact and dense nature	1289:1324	the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates	1289:1387	Microscopic studies revealed the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates.					
30584938	10	72	theme	active	1682:1687	arg1	films					1699:1703	active packaging films	1682:1703	active packaging films for food preservation	1682:1725	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	7	73	theme	content	1080:1086	arg1	terms					1056:1060	terms	1056:1060	terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging	1056:1162	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	5	74	theme	MLE	862:864	arg1	inclusion					866:874	MLE inclusion	862:874	MLE inclusion	862:874	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	7	75	from	MLE	1233:1235	arg1	films					1253:1257	the chitosan films	1240:1257	the chitosan films	1240:1257	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	8	76	theme	compact	1301:1307	arg1	nature					1319:1324	the smooth, compact and dense nature	1289:1324	the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates	1289:1387	Microscopic studies revealed the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates.					
30584938	7	77	theme	MLE	1233:1235	arg1	MLE					1233:1235	MLE	1233:1235	MLE in the chitosan films	1233:1257	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	7	77	theme	MLE	1233:1235	arg1	amounts					1222:1228	the incremental amounts	1206:1228	the incremental amounts of MLE in the chitosan films	1206:1257	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	7	78	theme	total	1065:1069	arg1	content					1080:1086	total phenolic content	1065:1086	total phenolic content	1065:1086	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	3	79	theme	films	580:584	arg1	morphology					476:485	morphology	476:485	morphology	476:485	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	3	79	theme	films	580:584	arg1	nature					496:501	optical nature	488:501	optical nature	488:501	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	3	79	theme	films	580:584	arg1	exposure					510:517	water exposure	504:517	water exposure	504:517	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	3	79	theme	films	580:584	arg1	characteristics					534:548	mechanical characteristics	523:548	mechanical characteristics	523:548	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	6	80	theme	reduced	956:962	arg1	ratio					975:979	reduced elongation ratio	956:979	reduced elongation ratio	956:979	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	7	81	theme	DPPH	1089:1092	arg1	scavenging					1102:1111	DPPH radical scavenging	1089:1111	DPPH radical scavenging	1089:1111	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	8	82	theme	dense	1313:1317	arg1	nature					1319:1324	the smooth, compact and dense nature	1289:1324	the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates	1289:1387	Microscopic studies revealed the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates.					
30584938	7	83	theme	Antioxidants	1029:1040	arg1	assessment					1042:1051	Antioxidants assessment	1029:1051	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging	1029:1162	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	0	84	theme	antioxidant	41:51	arg1	film					53:56	chitosan antioxidant film	32:56	chitosan antioxidant film for active food packaging	32:82	Mango leaf extract incorporated chitosan antioxidant film for active food packaging.					
30584938	9	85	theme	nuts	1420:1423	arg1	preservation					1425:1436	nuts preservation	1420:1436	nuts preservation for 28 days storage	1420:1456	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	7	86	from	films	1253:1257	arg1	MLE					1233:1235	MLE	1233:1235	MLE in the chitosan films	1233:1257	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	7	86	from	films	1253:1257	arg1	amounts					1222:1228	the incremental amounts	1206:1228	the incremental amounts of MLE in the chitosan films	1206:1257	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	6	87	contain	possessed	887:895	arg1	films					881:885	MLE films	877:885	MLE films	877:885	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	6	87	contain	possessed	887:895	arg2	strength					912:919	better tensile strength	897:919	better tensile strength	897:919	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	0	88	theme	food	69:72	arg1	packaging					74:82	active food packaging	62:82	active food packaging	62:82	Mango leaf extract incorporated chitosan antioxidant film for active food packaging.					
30584938	2	89	theme	reported	254:261	arg1	work					263:266	The reported work	250:266	The reported work	250:266	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	9	90	theme	polyamide/polyethylene	1538:1559	arg1	film					1561:1564	a commercial polyamide/polyethylene film	1525:1564	a commercial polyamide/polyethylene film	1525:1564	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	4	91	theme	decreased	680:688	arg1	content					699:705	decreased moisture content	680:705	decreased moisture content	680:705	Increase in the MLE concentration resulted in films with increased thickness and decreased moisture content.					
30584938	1	92	from	development	173:183	arg1	bio-sources					237:247	non-toxic and antioxidant rich bio-sources	206:247	non-toxic and antioxidant rich bio-sources	206:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	1	93	theme	preservation	148:159	arg1	demands					161:167	food preservation demands	143:167	food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources	143:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	7	94	theme	radical	1094:1100	arg1	scavenging					1102:1111	DPPH radical scavenging	1089:1111	DPPH radical scavenging	1089:1111	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	5	95	theme	solubility	729:738	arg1	analysis					763:770	Contact angle, water solubility and vapor permeability analysis	708:770	Contact angle, water solubility and vapor permeability analysis	708:770	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	8	96	theme	oxygen	1366:1371	arg1	rates					1383:1387	low oxygen transport rates	1362:1387	low oxygen transport rates	1362:1387	Microscopic studies revealed the smooth, compact and dense nature of the MLE-chitosan films favouring low oxygen transport rates.					
30584938	5	97	theme	vapor	744:748	arg1	permeability					750:761	vapor permeability	744:761	vapor permeability	744:761	Contact angle, water solubility and vapor permeability analysis demonstrated the reduced hydrophilicity and water vapor penetrability of the films due to MLE inclusion.					
30584938	3	98	dep	morphology	476:485	arg1	the					472:474	the	472:474	the	472:474	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	2	99	theme	extract	409:415	arg1	incorporation					423:435	mango leaf extract (MLE) incorporation	398:435	mango leaf extract (MLE) incorporation	398:435	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	0	100	theme	leaf	6:9	arg1	extract					11:17	Mango leaf extract	0:17	Mango leaf extract	0:17	Mango leaf extract incorporated chitosan antioxidant film for active food packaging.					
30584938	1	101	theme	Health	85:90	arg1	hazards					92:98	Health hazards	85:98	Health hazards	85:98	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	2	102	theme	mango	398:402	arg1	extract					409:415	mango leaf extract	398:415	mango leaf extract (MLE) incorporation	398:435	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	2	102	theme	mango	398:402	arg1	MLE					418:420	MLE	418:420	MLE	418:420	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	7	103	theme	improved	1171:1178	arg1	activity					1192:1199	improved antioxidant activity	1171:1199	improved antioxidant activity	1171:1199	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	1	104	theme	non-toxic	206:214	arg1	bio-sources					237:247	non-toxic and antioxidant rich bio-sources	206:247	non-toxic and antioxidant rich bio-sources	206:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	10	105	theme	food	1709:1712	arg1	preservation					1714:1725	food preservation	1709:1725	food preservation	1709:1725	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	7	106	theme	scavenging	1153:1162	arg1	terms					1056:1060	terms	1056:1060	terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging	1056:1162	Antioxidants assessment in terms of total phenolic content, DPPH radical scavenging, ferric reducing power and ABTS radical scavenging showed improved antioxidant activity with the incremental amounts of MLE in the chitosan films.					
30584938	9	107	theme	%	1509:1509	arg1	film					1515:1518	the 5% MLE film	1504:1518	the 5% MLE film than a commercial polyamide/polyethylene film	1504:1564	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30584938	6	108	dep	possessed	887:895	arg1	maximum					922:928	maximum	922:928	maximum of 23.06 ± 0.19 MPa	922:948	MLE films possessed better tensile strength (maximum of 23.06 ± 0.19 MPa) with reduced elongation ratio than the pure chitosan film (18.14 ± 0.72 MPa).					
30584938	4	109	with	films	645:649	arg1	content					699:705	decreased moisture content	680:705	decreased moisture content	680:705	Increase in the MLE concentration resulted in films with increased thickness and decreased moisture content.					
30584938	4	109	with	films	645:649	arg1	thickness					666:674	increased thickness	656:674	increased thickness	656:674	Increase in the MLE concentration resulted in films with increased thickness and decreased moisture content.					
30584938	3	110	from	Effect	438:443	arg1	morphology					476:485	morphology	476:485	morphology	476:485	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	3	110	from	Effect	438:443	arg1	nature					496:501	optical nature	488:501	optical nature	488:501	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	3	110	from	Effect	438:443	arg1	exposure					510:517	water exposure	504:517	water exposure	504:517	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	3	110	from	Effect	438:443	arg1	characteristics					534:548	mechanical characteristics	523:548	mechanical characteristics	523:548	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	2	111	theme	chitosan	340:347	arg1	films					349:353	chitosan films	340:353	chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation	340:435	The reported work highlights the development, characterization and application studies of chitosan films enhanced for their antioxidant activity by mango leaf extract (MLE) incorporation.					
30584938	1	112	theme	rich	232:235	arg1	bio-sources					237:247	non-toxic and antioxidant rich bio-sources	206:247	non-toxic and antioxidant rich bio-sources	206:247	Health hazards associated with usage of plastic films for food preservation demands for development of active films from non-toxic and antioxidant rich bio-sources.					
30584938	3	113	theme	variation	452:460	arg1	Effect					438:443	Effect	438:443	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films	438:584	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	4	114	theme	MLE	615:617	arg1	concentration					619:631	the MLE concentration	611:631	the MLE concentration	611:631	Increase in the MLE concentration resulted in films with increased thickness and decreased moisture content.					
30584938	10	115	theme	potential	1589:1597	arg1	nature					1613:1618	the potential and promising nature	1585:1618	the potential and promising nature of MLE impregnated chitosan films	1585:1652	Results highlight the potential and promising nature of MLE impregnated chitosan films as suitable alternative for active packaging films for food preservation.					
30584938	3	116	theme	water	504:508	arg1	exposure					510:517	water exposure	504:517	water exposure	504:517	Effect of MLE variation (1-5%) on the morphology, optical nature, water exposure and mechanical characteristics of the chitosan-MLE composite films was studied.					
30584938	9	117	theme	28 days	1442:1448	arg1	storage					1450:1456	28 days storage	1442:1456	28 days storage	1442:1456	Application studies to cashew nuts preservation for 28 days storage indicated 56% higher oxidation resistance for the 5% MLE film than a commercial polyamide/polyethylene film.					
30326284	6	0	theme	API	891:893	arg1	 w/w					910:913	10-30% w/w	904:913	10-30% w/w	904:913	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	6	0	theme	API	891:893	arg1	content					895:901	API content	891:901	API content (10-30% w/w)	891:914	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	15	1	dep	system	2064:2069	arg1	regard					2043:2048	regard	2043:2048	regard	2043:2048	With regard to the binary system of NMD with HPMC E5, surface crystallization was observed in VCM ASDs.					
30326284	13	2	theme	relevant	1828:1835	arg1	temperatures					1815:1826	temperatures	1815:1826	temperatures relevant for processing	1815:1850	The high viscosity and yield point of HPMC contributes to the mechanical robustness of the strand at temperatures relevant for processing.					
30326284	6	3	theme	phase	807:811	arg1	studies					813:819	phase studies	807:819	phase studies	807:819	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	8	4	theme	E	1141:1141	arg1	fraction					1143:1150	the Eudragit E fraction	1128:1150	the Eudragit E fraction of the formulation	1128:1169	A study of phase composition showed that the drug molecules were predominantly solubilized in the Eudragit E fraction of the formulation.					
30326284	6	5	theme	levels	881:886	arg1	range					847:851	a wide range	840:851	a wide range of polymer ratios and three levels of API content (10-30% w/w)	840:914	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	16	6	theme	polymer	2264:2270	arg1	ratios					2272:2277	different polymer ratios	2254:2277	different polymer ratios	2254:2277	For extruded pellets this was not the case, and a steady trend of formulation-intrinsic dissolution rate across different polymer ratios was observed.					
30326284	4	7	theme	physical	580:587	arg1	stability					589:597	physical stability	580:597	physical stability	580:597	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	16	8	theme	steady	2192:2197	arg1	trend					2199:2203	a steady trend	2190:2203	a steady trend of formulation-intrinsic dissolution rate across different polymer ratios	2190:2277	For extruded pellets this was not the case, and a steady trend of formulation-intrinsic dissolution rate across different polymer ratios was observed.					
30326284	1	9	theme	soluble	277:283	arg1	API					291:293	poorly soluble model API	270:293	poorly soluble model API in a dual-polymer carrier system	270:326	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	9	10	theme	miscibility	1176:1186	arg1	study					1188:1192	The miscibility study	1172:1192	The miscibility study	1172:1192	The miscibility study and Fourier-transform infrared spectroscopy indicated hydrogen (H)bond interactions between NMD and Eudragit E.					
30326284	4	11	from	processability	637:650	arg1	pelletization					662:674	strand pelletization	655:674	strand pelletization	655:674	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	8	12	theme	formulation	1159:1169	arg1	fraction					1143:1150	the Eudragit E fraction	1128:1150	the Eudragit E fraction of the formulation	1128:1169	A study of phase composition showed that the drug molecules were predominantly solubilized in the Eudragit E fraction of the formulation.					
30326284	6	13	theme	polymer	856:862	arg1	ratios					864:869	polymer ratios	856:869	polymer ratios	856:869	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	4	14	theme	strand	655:660	arg1	pelletization					662:674	strand pelletization	655:674	strand pelletization	655:674	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	1	15	from	API	291:293	arg1	system					321:326	a dual-polymer carrier system	298:326	a dual-polymer carrier system	298:326	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	11	16	theme	Eudragit	1563:1570	arg1	content					1574:1580	high Eudragit E content	1558:1580	high Eudragit E content	1558:1580	Concerning processability in strand pelletization the formulation is limited at high Eudragit E content.					
30326284	1	17	theme	solid	216:220	arg1	ASD					234:236	ASD	234:236	ASD	234:236	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	1	17	theme	solid	216:220	arg1	dispersion					222:231	an amorphous solid dispersion	203:231	an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system	203:326	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	18	18	theme	feasible	2425:2432	arg1	window					2446:2451	a feasible formulation window	2423:2451	a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC	2423:2501	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	16	19	theme	extruded	2146:2153	arg1	pellets					2155:2161	extruded pellets	2146:2161	extruded pellets	2146:2161	For extruded pellets this was not the case, and a steady trend of formulation-intrinsic dissolution rate across different polymer ratios was observed.					
30326284	0	20	theme	strand	137:142	arg1	pelletization					144:156	strand pelletization	137:156	strand pelletization	137:156	Formulation performance and processability window for manufacturing a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization.					
30326284	9	21	theme	infrared	1216:1223	arg1	spectroscopy					1225:1236	Fourier-transform infrared spectroscopy	1198:1236	Fourier-transform infrared spectroscopy	1198:1236	The miscibility study and Fourier-transform infrared spectroscopy indicated hydrogen (H)bond interactions between NMD and Eudragit E.					
30326284	12	22	theme	mixture	1618:1624	arg1	transition					1632:1641	low mixture glass transition	1614:1641	low mixture glass transition	1614:1641	NMD and EE-rich phases exhibit low mixture glass transition, low melt stability and brittle breaking behavior upon strand cutting.					
30326284	13	23	theme	yield	1737:1741	arg1	point					1743:1747	yield point	1737:1747	yield point	1737:1747	The high viscosity and yield point of HPMC contributes to the mechanical robustness of the strand at temperatures relevant for processing.					
30326284	6	24	theme	%	909:909	arg1	 w/w					910:913	10-30% w/w	904:913	10-30% w/w	904:913	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	6	24	theme	%	909:909	arg1	content					895:901	API content	891:901	API content (10-30% w/w)	891:914	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	18	25	theme	rate	2560:2563	arg1	enhancer					2565:2572	a dissolution rate enhancer and ASD stabilizer	2546:2591	enhancer	2565:2572	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	18	25	theme	rate	2560:2563	arg1	E					2536:2536	Eudragit E	2527:2536	Eudragit E	2527:2536	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	13	26	from	temperatures	1815:1826	arg1	robustness					1787:1796	the mechanical robustness	1772:1796	the mechanical robustness of the strand at temperatures relevant for processing	1772:1850	The high viscosity and yield point of HPMC contributes to the mechanical robustness of the strand at temperatures relevant for processing.					
30326284	17	27	theme	major	2325:2329	arg1	influence					2331:2339	a major influence	2323:2339	a major influence of shear stress during sample preparation on HPMC-based ASD performance	2323:2411	These discrepancies indicated a major influence of shear stress during sample preparation on HPMC-based ASD performance.					
30326284	14	28	theme	dissolution	1875:1885	arg1	rates					1887:1891	Formulation-intrinsic dissolution rates	1853:1891	Formulation-intrinsic dissolution rates in VCM ASDs	1853:1903	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	0	29	theme	dual-polymer	70:81	arg1	dispersion					99:108	a dual-polymer amorphous solid dispersion	68:108	a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization	68:156	Formulation performance and processability window for manufacturing a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization.					
30326284	10	30	theme	intermolecular	1431:1444	arg1	Hbonding					1446:1453	intermolecular Hbonding	1431:1453	intermolecular Hbonding between API molecules	1431:1475	In HPMC, the amorphous API was dispersed in polymeric matrix and stabilized due to anti-plasticization and the disruption of intermolecular Hbonding between API molecules.					
30326284	0	31	theme	solid	93:97	arg1	dispersion					99:108	a dual-polymer amorphous solid dispersion	68:108	a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization	68:156	Formulation performance and processability window for manufacturing a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization.					
30326284	7	32	theme	Ternary	917:923	arg1	formulations					929:940	Ternary ASD formulations	917:940	Ternary ASD formulations	917:940	Ternary ASD formulations were phase-separated, yet physically stable upon exposure to elevated temperature/humidity.					
30326284	13	33	theme	high	1718:1721	arg1	viscosity					1723:1731	high viscosity	1718:1731	high viscosity	1718:1731	The high viscosity and yield point of HPMC contributes to the mechanical robustness of the strand at temperatures relevant for processing.					
30326284	9	34	theme	Eudragit	1294:1301	arg1	E					1303:1303	Eudragit E	1294:1303	Eudragit E	1294:1303	The miscibility study and Fourier-transform infrared spectroscopy indicated hydrogen (H)bond interactions between NMD and Eudragit E.					
30326284	15	35	located	observed	2120:2127	arg2	crystallization					2100:2114	surface crystallization	2092:2114	surface crystallization	2092:2114	With regard to the binary system of NMD with HPMC E5, surface crystallization was observed in VCM ASDs.					
30326284	15	35	located	observed	2120:2127	arg1	ASDs					2136:2139	VCM ASDs	2132:2139	VCM ASDs	2132:2139	With regard to the binary system of NMD with HPMC E5, surface crystallization was observed in VCM ASDs.					
30326284	16	36	theme	dissolution	2230:2240	arg1	rate					2242:2245	formulation-intrinsic dissolution rate	2208:2245	formulation-intrinsic dissolution rate across different polymer ratios	2208:2277	For extruded pellets this was not the case, and a steady trend of formulation-intrinsic dissolution rate across different polymer ratios was observed.					
30326284	3	37	theme	hot-melt	427:434	arg1	HME					447:449	HME	447:449	HME	447:449	The formulation was designed for hot-melt extrusion (HME) and subsequent strand pelletization.					
30326284	3	37	theme	hot-melt	427:434	arg1	extrusion					436:444	hot-melt extrusion	427:444	hot-melt extrusion (HME)	427:450	The formulation was designed for hot-melt extrusion (HME) and subsequent strand pelletization.					
30326284	4	38	from	performance	562:572	arg1	pelletization					662:674	strand pelletization	655:674	strand pelletization	655:674	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	12	39	theme	breaking	1675:1682	arg1	behavior					1684:1691	brittle breaking behavior	1667:1691	brittle breaking behavior	1667:1691	NMD and EE-rich phases exhibit low mixture glass transition, low melt stability and brittle breaking behavior upon strand cutting.					
30326284	0	40	theme	amorphous	83:91	arg1	dispersion					99:108	a dual-polymer amorphous solid dispersion	68:108	a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization	68:156	Formulation performance and processability window for manufacturing a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization.					
30326284	0	41	theme	Formulation	0:10	arg1	performance					12:22	Formulation performance	0:22	Formulation performance	0:22	Formulation performance and processability window for manufacturing a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization.					
30326284	1	42	theme	model	285:289	arg1	API					291:293	poorly soluble model API	270:293	poorly soluble model API in a dual-polymer carrier system	270:326	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	4	43	dep	stability	589:597	arg1	i.e.					575:578	i.e.	575:578	i.e.	575:578	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	15	44	theme	VCM	2132:2134	arg1	ASDs					2136:2139	VCM ASDs	2132:2139	VCM ASDs	2132:2139	With regard to the binary system of NMD with HPMC E5, surface crystallization was observed in VCM ASDs.					
30326284	8	45	theme	phase	1045:1049	arg1	composition					1051:1061	phase composition	1045:1061	phase composition	1045:1061	A study of phase composition showed that the drug molecules were predominantly solubilized in the Eudragit E fraction of the formulation.					
30326284	15	46	theme	binary	2057:2062	arg1	system					2064:2069	the binary system	2053:2069	the binary system of NMD with HPMC E5	2053:2089	With regard to the binary system of NMD with HPMC E5, surface crystallization was observed in VCM ASDs.					
30326284	12	47	theme	melt	1648:1651	arg1	stability					1653:1661	low melt stability	1644:1661	low melt stability	1644:1661	NMD and EE-rich phases exhibit low mixture glass transition, low melt stability and brittle breaking behavior upon strand cutting.					
30326284	4	48	theme	functional	547:556	arg1	performance					562:572	desired functional ASD performance	539:572	desired functional ASD performance	539:572	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	1	49	theme	dual-polymer	300:311	arg1	system					321:326	a dual-polymer carrier system	298:326	a dual-polymer carrier system	298:326	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	6	50	theme	wide	842:845	arg1	range					847:851	a wide range	840:851	a wide range of polymer ratios and three levels of API content (10-30% w/w)	840:914	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	14	51	theme	competitive	1986:1996	arg1	mechanisms					2010:2019	diverse and competitive dissolution mechanisms	1974:2019	diverse and competitive dissolution mechanisms in the polymers	1974:2035	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	18	52	theme	E	2496:2496	arg1	ratio					2470:2474	a polymer ratio	2460:2474	a polymer ratio of 1:2-2:3 Eudragit E	2460:2496	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	2	53	theme	Eudragit	341:348	arg1	E					350:350	Eudragit E	341:350	Eudragit E	341:350	HPMC E5 and Eudragit E were used for the two polymeric carriers.					
30326284	14	54	theme	diverse	1974:1980	arg1	mechanisms					2010:2019	diverse and competitive dissolution mechanisms	1974:2019	diverse and competitive dissolution mechanisms in the polymers	1974:2035	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	4	55	theme	formulation	515:525	arg1	window					527:532	a formulation window	513:532	a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization	513:674	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	18	56	theme	1:2-2:3	2479:2485	arg1	E					2496:2496	1:2-2:3 Eudragit E	2479:2496	1:2-2:3 Eudragit E	2479:2496	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	3	57	theme	subsequent	456:465	arg1	pelletization					474:486	subsequent strand pelletization	456:486	subsequent strand pelletization	456:486	The formulation was designed for hot-melt extrusion (HME) and subsequent strand pelletization.					
30326284	17	58	theme	stress	2350:2355	arg1	influence					2331:2339	a major influence	2323:2339	a major influence of shear stress during sample preparation on HPMC-based ASD performance	2323:2411	These discrepancies indicated a major influence of shear stress during sample preparation on HPMC-based ASD performance.					
30326284	13	59	theme	strand	1805:1810	arg1	robustness					1787:1796	the mechanical robustness	1772:1796	the mechanical robustness of the strand at temperatures relevant for processing	1772:1850	The high viscosity and yield point of HPMC contributes to the mechanical robustness of the strand at temperatures relevant for processing.					
30326284	6	60	theme	content	895:901	arg1	ratios					864:869	polymer ratios	856:869	polymer ratios	856:869	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	6	60	theme	content	895:901	arg1	levels					881:886	three levels	875:886	three levels of API content (10-30% w/w)	875:914	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	14	61	theme	ratio	1951:1955	arg1	function					1931:1938	an irregular function	1918:1938	an irregular function	1918:1938	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	18	62	theme	dissolution	2548:2558	arg1	enhancer					2565:2572	a dissolution rate enhancer and ASD stabilizer	2546:2591	enhancer	2565:2572	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	18	62	theme	dissolution	2548:2558	arg1	E					2536:2536	Eudragit E	2527:2536	Eudragit E	2527:2536	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	4	63	theme	immediate	603:611	arg1	release					617:623	immediate API release	603:623	immediate API release	603:623	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	13	64	theme	mechanical	1776:1785	arg1	robustness					1787:1796	the mechanical robustness	1772:1796	the mechanical robustness of the strand at temperatures relevant for processing	1772:1850	The high viscosity and yield point of HPMC contributes to the mechanical robustness of the strand at temperatures relevant for processing.					
30326284	12	65	dep	NMD	1583:1585	arg1	phases					1599:1604	phases	1599:1604	phases	1599:1604	NMD and EE-rich phases exhibit low mixture glass transition, low melt stability and brittle breaking behavior upon strand cutting.					
30326284	11	66	theme	high	1558:1561	arg1	content					1574:1580	high Eudragit E content	1558:1580	high Eudragit E content	1558:1580	Concerning processability in strand pelletization the formulation is limited at high Eudragit E content.					
30326284	8	67	theme	Eudragit	1132:1139	arg1	fraction					1143:1150	the Eudragit E fraction	1128:1150	the Eudragit E fraction of the formulation	1128:1169	A study of phase composition showed that the drug molecules were predominantly solubilized in the Eudragit E fraction of the formulation.					
30326284	17	68	theme	ASD	2397:2399	arg1	performance					2401:2411	HPMC-based ASD performance	2386:2411	HPMC-based ASD performance	2386:2411	These discrepancies indicated a major influence of shear stress during sample preparation on HPMC-based ASD performance.					
30326284	16	69	theme	formulation-intrinsic	2208:2228	arg1	rate					2242:2245	formulation-intrinsic dissolution rate	2208:2245	formulation-intrinsic dissolution rate across different polymer ratios	2208:2277	For extruded pellets this was not the case, and a steady trend of formulation-intrinsic dissolution rate across different polymer ratios was observed.					
30326284	10	70	theme	polymeric	1350:1358	arg1	matrix					1360:1365	polymeric matrix	1350:1365	polymeric matrix	1350:1365	In HPMC, the amorphous API was dispersed in polymeric matrix and stabilized due to anti-plasticization and the disruption of intermolecular Hbonding between API molecules.					
30326284	14	71	theme	polymer	1943:1949	arg1	ratio					1951:1955	polymer ratio	1943:1955	polymer ratio	1943:1955	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	6	72	theme	ratios	864:869	arg1	range					847:851	a wide range	840:851	a wide range of polymer ratios and three levels of API content (10-30% w/w)	840:914	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	13	73	theme	HPMC	1752:1755	arg1	point					1743:1747	yield point	1737:1747	yield point	1737:1747	The high viscosity and yield point of HPMC contributes to the mechanical robustness of the strand at temperatures relevant for processing.					
30326284	13	73	theme	HPMC	1752:1755	arg1	viscosity					1723:1731	high viscosity	1718:1731	high viscosity	1718:1731	The high viscosity and yield point of HPMC contributes to the mechanical robustness of the strand at temperatures relevant for processing.					
30326284	4	74	with	window	527:532	arg1	processability					637:650	processability	637:650	desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization	539:674	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	4	74	with	window	527:532	arg1	performance					562:572	desired functional ASD performance	539:572	desired functional ASD performance	539:572	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	1	75	theme	amorphous	206:214	arg1	ASD					234:236	ASD	234:236	ASD	234:236	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	1	75	theme	amorphous	206:214	arg1	dispersion					222:231	an amorphous solid dispersion	203:231	an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system	203:326	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	13	76	dep	viscosity	1723:1731	arg1	The					1714:1716	The	1714:1716	The	1714:1716	The high viscosity and yield point of HPMC contributes to the mechanical robustness of the strand at temperatures relevant for processing.					
30326284	12	77	theme	low	1614:1616	arg1	transition					1632:1641	low mixture glass transition	1614:1641	low mixture glass transition	1614:1641	NMD and EE-rich phases exhibit low mixture glass transition, low melt stability and brittle breaking behavior upon strand cutting.					
30326284	18	78	theme	formulation	2434:2444	arg1	window					2446:2451	a feasible formulation window	2423:2451	a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC	2423:2501	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	15	79	with	system	2064:2069	arg1	E5					2088:2089	HPMC E5	2083:2089	HPMC E5	2083:2089	With regard to the binary system of NMD with HPMC E5, surface crystallization was observed in VCM ASDs.					
30326284	9	80	theme	Fourier-transform	1198:1214	arg1	spectroscopy					1225:1236	Fourier-transform infrared spectroscopy	1198:1236	Fourier-transform infrared spectroscopy	1198:1236	The miscibility study and Fourier-transform infrared spectroscopy indicated hydrogen (H)bond interactions between NMD and Eudragit E.					
30326284	1	81	theme	dispersion	222:231	arg1	compositions					187:198	several compositions	179:198	several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system	179:326	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	12	82	theme	glass	1626:1630	arg1	transition					1632:1641	low mixture glass transition	1614:1641	low mixture glass transition	1614:1641	NMD and EE-rich phases exhibit low mixture glass transition, low melt stability and brittle breaking behavior upon strand cutting.					
30326284	18	83	theme	ASD	2578:2580	arg1	stabilizer					2582:2591	a dissolution rate enhancer and ASD stabilizer	2546:2591	stabilizer	2582:2591	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	5	84	theme	small-scale	705:715	arg1	molding					753:759	vacuum compression molding	734:759	vacuum compression molding (VCM)	734:765	Samples were prepared using small-scale methods, such as vacuum compression molding (VCM) and benchtop extrusion.					
30326284	5	84	theme	small-scale	705:715	arg1	extrusion					780:788	benchtop extrusion	771:788	benchtop extrusion	771:788	Samples were prepared using small-scale methods, such as vacuum compression molding (VCM) and benchtop extrusion.					
30326284	5	84	theme	small-scale	705:715	arg1	methods					717:723	small-scale methods	705:723	small-scale methods	705:723	Samples were prepared using small-scale methods, such as vacuum compression molding (VCM) and benchtop extrusion.					
30326284	14	85	theme	irregular	1921:1929	arg1	function					1931:1938	an irregular function	1918:1938	an irregular function	1918:1938	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	7	86	theme	elevated	1003:1010	arg1	temperature/humidity					1012:1031	elevated temperature/humidity	1003:1031	elevated temperature/humidity	1003:1031	Ternary ASD formulations were phase-separated, yet physically stable upon exposure to elevated temperature/humidity.					
30326284	9	87	theme	hydrogen	1248:1255	arg1	interactions					1265:1276	hydrogen (H)bond interactions	1248:1276	hydrogen (H)bond interactions between NMD and Eudragit E	1248:1303	The miscibility study and Fourier-transform infrared spectroscopy indicated hydrogen (H)bond interactions between NMD and Eudragit E.					
30326284	14	88	theme	VCM	1896:1898	arg1	ASDs					1900:1903	VCM ASDs	1896:1903	VCM ASDs	1896:1903	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	6	89	theme	10-30	904:908	arg1	%					909:909	%	909:909	%	909:909	Miscibility and phase studies were performed for a wide range of polymer ratios and three levels of API content (10-30% w/w).					
30326284	11	90	theme	E	1572:1572	arg1	content					1574:1580	high Eudragit E content	1558:1580	high Eudragit E content	1558:1580	Concerning processability in strand pelletization the formulation is limited at high Eudragit E content.					
30326284	9	91	theme	H	1258:1258	arg1	interactions					1265:1276	hydrogen (H)bond interactions	1248:1276	hydrogen (H)bond interactions between NMD and Eudragit E	1248:1303	The miscibility study and Fourier-transform infrared spectroscopy indicated hydrogen (H)bond interactions between NMD and Eudragit E.					
30326284	18	92	dep	window	2446:2451	arg1	HPMC					2498:2501	HPMC	2498:2501	a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC	2423:2501	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	5	93	theme	compression	741:751	arg1	molding					753:759	vacuum compression molding	734:759	vacuum compression molding (VCM)	734:765	Samples were prepared using small-scale methods, such as vacuum compression molding (VCM) and benchtop extrusion.					
30326284	5	93	theme	compression	741:751	arg1	VCM					762:764	VCM	762:764	VCM	762:764	Samples were prepared using small-scale methods, such as vacuum compression molding (VCM) and benchtop extrusion.					
30326284	9	94	theme	bond	1260:1263	arg1	interactions					1265:1276	hydrogen (H)bond interactions	1248:1276	hydrogen (H)bond interactions between NMD and Eudragit E	1248:1303	The miscibility study and Fourier-transform infrared spectroscopy indicated hydrogen (H)bond interactions between NMD and Eudragit E.					
30326284	14	95	theme	Formulation-intrinsic	1853:1873	arg1	rates					1887:1891	Formulation-intrinsic dissolution rates	1853:1891	Formulation-intrinsic dissolution rates in VCM ASDs	1853:1903	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	17	96	from	influence	2331:2339	arg1	performance					2401:2411	HPMC-based ASD performance	2386:2411	HPMC-based ASD performance	2386:2411	These discrepancies indicated a major influence of shear stress during sample preparation on HPMC-based ASD performance.					
30326284	1	97	theme	several	179:185	arg1	compositions					187:198	several compositions	179:198	several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system	179:326	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	11	98	theme	strand	1507:1512	arg1	pelletization					1514:1526	strand pelletization	1507:1526	strand pelletization	1507:1526	Concerning processability in strand pelletization the formulation is limited at high Eudragit E content.					
30326284	0	99	theme	hot-melt	114:121	arg1	extrusion					123:131	hot-melt extrusion	114:131	hot-melt extrusion	114:131	Formulation performance and processability window for manufacturing a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization.					
30326284	7	100	theme	ASD	925:927	arg1	formulations					929:940	Ternary ASD formulations	917:940	Ternary ASD formulations	917:940	Ternary ASD formulations were phase-separated, yet physically stable upon exposure to elevated temperature/humidity.					
30326284	15	101	theme	HPMC	2083:2086	arg1	E5					2088:2089	HPMC E5	2083:2089	HPMC E5	2083:2089	With regard to the binary system of NMD with HPMC E5, surface crystallization was observed in VCM ASDs.					
30326284	2	102	used	used	357:360	arg2	E					350:350	Eudragit E	341:350	Eudragit E	341:350	HPMC E5 and Eudragit E were used for the two polymeric carriers.					
30326284	2	102	used	used	357:360	arg2	E5					334:335	HPMC E5	329:335	HPMC E5	329:335	HPMC E5 and Eudragit E were used for the two polymeric carriers.					
30326284	16	103	theme	rate	2242:2245	arg1	trend					2199:2203	a steady trend	2190:2203	a steady trend of formulation-intrinsic dissolution rate across different polymer ratios	2190:2277	For extruded pellets this was not the case, and a steady trend of formulation-intrinsic dissolution rate across different polymer ratios was observed.					
30326284	3	104	theme	strand	467:472	arg1	pelletization					474:486	subsequent strand pelletization	456:486	subsequent strand pelletization	456:486	The formulation was designed for hot-melt extrusion (HME) and subsequent strand pelletization.					
30326284	15	105	theme	NMD	2074:2076	arg1	system					2064:2069	the binary system	2053:2069	the binary system of NMD with HPMC E5	2053:2089	With regard to the binary system of NMD with HPMC E5, surface crystallization was observed in VCM ASDs.					
30326284	10	106	theme	API	1463:1465	arg1	molecules					1467:1475	API molecules	1463:1475	API molecules	1463:1475	In HPMC, the amorphous API was dispersed in polymeric matrix and stabilized due to anti-plasticization and the disruption of intermolecular Hbonding between API molecules.					
30326284	16	107	theme	different	2254:2262	arg1	ratios					2272:2277	different polymer ratios	2254:2277	different polymer ratios	2254:2277	For extruded pellets this was not the case, and a steady trend of formulation-intrinsic dissolution rate across different polymer ratios was observed.					
30326284	2	108	theme	polymeric	374:382	arg1	carriers					384:391	the two polymeric carriers	366:391	the two polymeric carriers	366:391	HPMC E5 and Eudragit E were used for the two polymeric carriers.					
30326284	14	109	from	mechanisms	2010:2019	arg1	polymers					2028:2035	the polymers	2024:2035	the polymers	2024:2035	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	12	110	theme	brittle	1667:1673	arg1	behavior					1684:1691	brittle breaking behavior	1667:1691	brittle breaking behavior	1667:1691	NMD and EE-rich phases exhibit low mixture glass transition, low melt stability and brittle breaking behavior upon strand cutting.					
30326284	4	111	theme	desired	539:545	arg1	performance					562:572	desired functional ASD performance	539:572	desired functional ASD performance	539:572	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	10	112	theme	Hbonding	1446:1453	arg1	anti-plasticization					1389:1407	anti-plasticization	1389:1407	anti-plasticization	1389:1407	In HPMC, the amorphous API was dispersed in polymeric matrix and stabilized due to anti-plasticization and the disruption of intermolecular Hbonding between API molecules.					
30326284	10	112	theme	Hbonding	1446:1453	arg1	disruption					1417:1426	the disruption	1413:1426	the disruption of intermolecular Hbonding between API molecules	1413:1475	In HPMC, the amorphous API was dispersed in polymeric matrix and stabilized due to anti-plasticization and the disruption of intermolecular Hbonding between API molecules.					
30326284	0	113	theme	processability	28:41	arg1	window					43:48	processability window	28:48	processability window	28:48	Formulation performance and processability window for manufacturing a dual-polymer amorphous solid dispersion via hot-melt extrusion and strand pelletization.					
30326284	8	114	theme	composition	1051:1061	arg1	study					1036:1040	A study	1034:1040	A study of phase composition	1034:1061	A study of phase composition showed that the drug molecules were predominantly solubilized in the Eudragit E fraction of the formulation.					
30326284	11	115	from	processability	1489:1502	arg1	pelletization					1514:1526	strand pelletization	1507:1526	strand pelletization	1507:1526	Concerning processability in strand pelletization the formulation is limited at high Eudragit E content.					
30326284	4	116	theme	ASD	558:560	arg1	performance					562:572	desired functional ASD performance	539:572	desired functional ASD performance	539:572	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	18	117	theme	polymer	2462:2468	arg1	ratio					2470:2474	a polymer ratio	2460:2474	a polymer ratio of 1:2-2:3 Eudragit E	2460:2496	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	14	118	theme	dissolution	1998:2008	arg1	mechanisms					2010:2019	diverse and competitive dissolution mechanisms	1974:2019	diverse and competitive dissolution mechanisms in the polymers	1974:2035	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	12	119	theme	low	1644:1646	arg1	stability					1653:1661	low melt stability	1644:1661	low melt stability	1644:1661	NMD and EE-rich phases exhibit low mixture glass transition, low melt stability and brittle breaking behavior upon strand cutting.					
30326284	10	120	theme	amorphous	1319:1327	arg1	API					1329:1331	the amorphous API	1315:1331	the amorphous API	1315:1331	In HPMC, the amorphous API was dispersed in polymeric matrix and stabilized due to anti-plasticization and the disruption of intermolecular Hbonding between API molecules.					
30326284	5	121	theme	vacuum	734:739	arg1	molding					753:759	vacuum compression molding	734:759	vacuum compression molding (VCM)	734:765	Samples were prepared using small-scale methods, such as vacuum compression molding (VCM) and benchtop extrusion.					
30326284	5	121	theme	vacuum	734:739	arg1	VCM					762:764	VCM	762:764	VCM	762:764	Samples were prepared using small-scale methods, such as vacuum compression molding (VCM) and benchtop extrusion.					
30326284	5	122	theme	benchtop	771:778	arg1	extrusion					780:788	benchtop extrusion	771:788	benchtop extrusion	771:788	Samples were prepared using small-scale methods, such as vacuum compression molding (VCM) and benchtop extrusion.					
30326284	1	123	theme	carrier	313:319	arg1	system					321:326	a dual-polymer carrier system	298:326	a dual-polymer carrier system	298:326	This work evaluates several compositions of an amorphous solid dispersion (ASD) comprising nimodipine (NMD) as poorly soluble model API in a dual-polymer carrier system.					
30326284	8	124	theme	drug	1079:1082	arg1	molecules					1084:1092	the drug molecules	1075:1092	the drug molecules	1075:1092	A study of phase composition showed that the drug molecules were predominantly solubilized in the Eudragit E fraction of the formulation.					
30326284	18	125	theme	Eudragit	2487:2494	arg1	E					2496:2496	1:2-2:3 Eudragit E	2479:2496	1:2-2:3 Eudragit E	2479:2496	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	18	126	theme	Eudragit	2527:2534	arg1	enhancer					2565:2572	a dissolution rate enhancer and ASD stabilizer	2546:2591	enhancer	2565:2572	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	18	126	theme	Eudragit	2527:2534	arg1	E					2536:2536	Eudragit E	2527:2536	Eudragit E	2527:2536	Finally, a feasible formulation window within a polymer ratio of 1:2-2:3 Eudragit E:HPMC was identified in which Eudragit E acts as a dissolution rate enhancer and ASD stabilizer during dissolution.					
30326284	14	127	from	rates	1887:1891	arg1	ASDs					1900:1903	VCM ASDs	1896:1903	VCM ASDs	1896:1903	Formulation-intrinsic dissolution rates in VCM ASDs developed as an irregular function of polymer ratio, associated with diverse and competitive dissolution mechanisms in the polymers.					
30326284	15	128	theme	surface	2092:2098	arg1	crystallization					2100:2114	surface crystallization	2092:2114	surface crystallization	2092:2114	With regard to the binary system of NMD with HPMC E5, surface crystallization was observed in VCM ASDs.					
30326284	11	129	dep	limited	1547:1553	arg1	Concerning					1478:1487	Concerning	1478:1487	Concerning processability in strand pelletization	1478:1526	Concerning processability in strand pelletization the formulation is limited at high Eudragit E content.					
30326284	17	130	theme	shear	2344:2348	arg1	stress					2350:2355	shear stress	2344:2355	shear stress	2344:2355	These discrepancies indicated a major influence of shear stress during sample preparation on HPMC-based ASD performance.					
30326284	17	131	theme	HPMC-based	2386:2395	arg1	performance					2401:2411	HPMC-based ASD performance	2386:2411	HPMC-based ASD performance	2386:2411	These discrepancies indicated a major influence of shear stress during sample preparation on HPMC-based ASD performance.					
30326284	2	132	theme	HPMC	329:332	arg1	E5					334:335	HPMC E5	329:335	HPMC E5	329:335	HPMC E5 and Eudragit E were used for the two polymeric carriers.					
30326284	4	133	theme	API	613:615	arg1	release					617:623	immediate API release	603:623	immediate API release	603:623	The aim was to identify a formulation window with desired functional ASD performance, i.e. physical stability and immediate API release, as well as processability in strand pelletization.					
30326284	17	134	theme	sample	2364:2369	arg1	preparation					2371:2381	sample preparation	2364:2381	sample preparation	2364:2381	These discrepancies indicated a major influence of shear stress during sample preparation on HPMC-based ASD performance.					
31336282	5	0	theme	myocardin	1157:1165	arg1	expression					1143:1152	the expression	1139:1152	the expression of myocardin and α-smooth muscle actin	1139:1191	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	4	1	theme	low	726:728	arg1	cytotoxicity					730:741	low cytotoxicity	726:741	low cytotoxicity	726:741	The TMC-g-PEG-VAPG/miRNA-145 complexes exhibited low cytotoxicity, and TMC-g-PEG-VAPG with relatively higher molecular weight of chitosan (50 kDa) could significantly enhance cellular uptake in SMCs.					
31336282	7	2	theme	small-diameter	1515:1528	arg1	regeneration					1543:1554	small-diameter blood vessel regeneration	1515:1554	small-diameter blood vessel regeneration	1515:1554	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	1	3	theme	vascular	177:184	arg1	regeneration					186:197	small-diameter vascular regeneration	162:197	small-diameter vascular regeneration	162:197	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	6	4	attach	released	1217:1224	arg1	membranes					1247:1255	the electrospun membranes	1231:1255	the electrospun membranes	1231:1255	Furthermore, miRNA-145 released from the electrospun membranes also showed in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration, at least 56 days.					
31336282	6	4	attach	released	1217:1224	arg2	miRNA-145					1207:1215	miRNA-145	1207:1215	miRNA-145 released from the electrospun membranes	1207:1255	Furthermore, miRNA-145 released from the electrospun membranes also showed in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration, at least 56 days.					
31336282	2	5	theme	intimal	469:475	arg1	hyperplasia					477:487	intimal hyperplasia	469:487	intimal hyperplasia	469:487	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	7	6	theme	regeneration	1543:1554	arg1	application					1500:1510	the application	1496:1510	the application of small-diameter blood vessel regeneration	1496:1554	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	6	7	theme	SMCs	1333:1336	arg1	phenotype					1320:1328	the contractile phenotype	1304:1328	the contractile phenotype of SMCs in the prolonged duration	1304:1362	Furthermore, miRNA-145 released from the electrospun membranes also showed in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration, at least 56 days.					
31336282	5	8	theme	electrospun	940:950	arg1	membranes					952:960	the electrospun membranes	936:960	the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone)	936:1021	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	5	8	theme	electrospun	940:950	arg1	complexes					925:933	TMC-g-PEG-VAPG/miRNA-145 complexes	900:933	TMC-g-PEG-VAPG/miRNA-145 complexes	900:933	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	3	9	theme	VAPG	498:501	arg1	chitosan-g-poly					530:544	VAPG peptide-modified trimethyl chitosan-g-poly	498:544	VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG)	498:578	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	3	9	theme	VAPG	498:501	arg1	glycol					555:560	ethylene glycol	546:560	ethylene glycol	546:560	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	3	9	theme	VAPG	498:501	arg1	TMC-g-PEG-VAPG					564:577	TMC-g-PEG-VAPG	564:577	TMC-g-PEG-VAPG	564:577	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	0	10	from	Performance	0:10	arg1	membranes					65:73	electrospun membranes	53:73	electrospun membranes	53:73	Performance of TMC-g-PEG-VAPG/miRNA-145 complexes in electrospun membranes for target-regulating vascular SMCs.					
31336282	2	11	from	microRNAs	320:328	arg1	systems					351:357	vascular systems	342:357	vascular systems	342:357	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	3	12	theme	ethylene	546:553	arg1	chitosan-g-poly					530:544	VAPG peptide-modified trimethyl chitosan-g-poly	498:544	VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG)	498:578	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	3	12	theme	ethylene	546:553	arg1	glycol					555:560	ethylene glycol	546:560	ethylene glycol	546:560	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	7	13	theme	blood	1530:1534	arg1	regeneration					1543:1554	small-diameter blood vessel regeneration	1515:1554	small-diameter blood vessel regeneration	1515:1554	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	2	14	theme	vascular	342:349	arg1	systems					351:357	vascular systems	342:357	vascular systems	342:357	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	1	15	theme	challenges	148:157	arg1	challenges					148:157	the main challenges	139:157	the main challenges in small-diameter vascular regeneration	139:197	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	1	15	theme	challenges	148:157	arg1	one					132:134	one	132:134	one	132:134	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	6	16	theme	prolonged	1345:1353	arg1	duration					1355:1362	the prolonged duration	1341:1362	the prolonged duration	1341:1362	Furthermore, miRNA-145 released from the electrospun membranes also showed in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration, at least 56 days.					
31336282	6	17	theme	contractile	1308:1318	arg1	phenotype					1320:1328	the contractile phenotype	1304:1328	the contractile phenotype of SMCs in the prolonged duration	1304:1362	Furthermore, miRNA-145 released from the electrospun membranes also showed in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration, at least 56 days.					
31336282	1	18	theme	vascular	228:235	arg1	SMCs					258:261	SMCs	258:261	SMCs	258:261	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	1	18	theme	vascular	228:235	arg1	cells					251:255	vascular smooth muscle cells	228:255	vascular smooth muscle cells (SMCs)	228:262	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	7	19	contain	containing	1420:1429	arg2	complexes					1456:1464	TMC-g-PEG-VAPG/miRNA-145 complexes	1431:1464	TMC-g-PEG-VAPG/miRNA-145 complexes	1431:1464	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	7	19	contain	containing	1420:1429	arg1	membranes					1410:1418	The functional electrospun membranes	1383:1418	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes	1383:1464	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	4	20	theme	chitosan	806:813	arg1	weight					796:801	relatively higher molecular weight	768:801	relatively higher molecular weight of chitosan (50 kDa)	768:822	The TMC-g-PEG-VAPG/miRNA-145 complexes exhibited low cytotoxicity, and TMC-g-PEG-VAPG with relatively higher molecular weight of chitosan (50 kDa) could significantly enhance cellular uptake in SMCs.					
31336282	6	21	dep	in	1269:1270	arg1	vitro					1272:1276	vitro	1272:1276	vitro	1272:1276	Furthermore, miRNA-145 released from the electrospun membranes also showed in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration, at least 56 days.					
31336282	7	22	theme	TMC-g-PEG-VAPG/miRNA-145	1431:1454	arg1	complexes					1456:1464	TMC-g-PEG-VAPG/miRNA-145 complexes	1431:1464	TMC-g-PEG-VAPG/miRNA-145 complexes	1431:1464	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	3	23	theme	trimethyl	520:528	arg1	chitosan-g-poly					530:544	VAPG peptide-modified trimethyl chitosan-g-poly	498:544	VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG)	498:578	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	3	23	theme	trimethyl	520:528	arg1	glycol					555:560	ethylene glycol	546:560	ethylene glycol	546:560	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	3	23	theme	trimethyl	520:528	arg1	TMC-g-PEG-VAPG					564:577	TMC-g-PEG-VAPG	564:577	TMC-g-PEG-VAPG	564:577	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	1	24	from	challenges	148:157	arg1	regeneration					186:197	small-diameter vascular regeneration	162:197	small-diameter vascular regeneration	162:197	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	7	25	theme	vessel	1536:1541	arg1	regeneration					1543:1554	small-diameter blood vessel regeneration	1515:1554	small-diameter blood vessel regeneration	1515:1554	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	1	26	theme	effective	204:212	arg1	modulation					214:223	effective modulation	204:223	effective modulation of vascular smooth muscle cells (SMCs)	204:262	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	7	27	contain	have	1470:1473	arg2	potential					1483:1491	a great potential	1475:1491	a great potential	1475:1491	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	7	27	contain	have	1470:1473	arg1	membranes					1410:1418	The functional electrospun membranes	1383:1418	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes	1383:1464	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	7	28	theme	great	1477:1481	arg1	potential					1483:1491	a great potential	1475:1491	a great potential	1475:1491	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	7	29	theme	electrospun	1398:1408	arg1	membranes					1410:1418	The functional electrospun membranes	1383:1418	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes	1383:1464	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	0	30	theme	complexes	40:48	arg1	Performance					0:10	Performance	0:10	Performance of TMC-g-PEG-VAPG/miRNA-145 complexes in electrospun membranes for target-regulating vascular SMCs.	0:110	Performance of TMC-g-PEG-VAPG/miRNA-145 complexes in electrospun membranes for target-regulating vascular SMCs.					
31336282	6	31	theme	electrospun	1235:1245	arg1	membranes					1247:1255	the electrospun membranes	1231:1255	the electrospun membranes	1231:1255	Furthermore, miRNA-145 released from the electrospun membranes also showed in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration, at least 56 days.					
31336282	5	32	theme	ethylene	970:977	arg1	poly					965:968	poly	965:968	poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone)	965:1021	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	5	32	theme	ethylene	970:977	arg1	glycol					979:984	ethylene glycol	970:984	ethylene glycol	970:984	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	4	33	theme	molecular	786:794	arg1	weight					796:801	relatively higher molecular weight	768:801	relatively higher molecular weight of chitosan (50 kDa)	768:822	The TMC-g-PEG-VAPG/miRNA-145 complexes exhibited low cytotoxicity, and TMC-g-PEG-VAPG with relatively higher molecular weight of chitosan (50 kDa) could significantly enhance cellular uptake in SMCs.					
31336282	5	34	theme	gene	1059:1062	arg1	levels					1076:1081	gene and protein levels	1059:1081	levels	1076:1081	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	2	35	theme	contractile	402:412	arg1	phenotype					414:422	the normal contractile phenotype	391:422	the normal contractile phenotype	391:422	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	0	36	theme	TMC-g-PEG-VAPG/miRNA-145	15:38	arg1	complexes					40:48	TMC-g-PEG-VAPG/miRNA-145 complexes	15:48	TMC-g-PEG-VAPG/miRNA-145 complexes	15:48	Performance of TMC-g-PEG-VAPG/miRNA-145 complexes in electrospun membranes for target-regulating vascular SMCs.					
31336282	1	37	theme	related	294:300	arg1	issues					302:307	the related issues	290:307	the related issues	290:307	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	4	38	theme	higher	779:784	arg1	weight					796:801	relatively higher molecular weight	768:801	relatively higher molecular weight of chitosan (50 kDa)	768:822	The TMC-g-PEG-VAPG/miRNA-145 complexes exhibited low cytotoxicity, and TMC-g-PEG-VAPG with relatively higher molecular weight of chitosan (50 kDa) could significantly enhance cellular uptake in SMCs.					
31336282	2	39	theme	normal	395:400	arg1	phenotype					414:422	the normal contractile phenotype	391:422	the normal contractile phenotype	391:422	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	0	40	theme	electrospun	53:63	arg1	membranes					65:73	electrospun membranes	53:73	electrospun membranes	53:73	Performance of TMC-g-PEG-VAPG/miRNA-145 complexes in electrospun membranes for target-regulating vascular SMCs.					
31336282	6	41	theme	in	1269:1270	arg1	bioactivity					1278:1288	in vitro bioactivity	1269:1288	in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration	1269:1362	Furthermore, miRNA-145 released from the electrospun membranes also showed in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration, at least 56 days.					
31336282	5	42	theme	Krüppel-like	1105:1116	arg1	factor					1118:1123	Krüppel-like factor 4	1105:1125	Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin	1105:1191	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	4	43	theme	cellular	852:859	arg1	uptake					861:866	cellular uptake	852:866	cellular uptake in SMCs	852:874	The TMC-g-PEG-VAPG/miRNA-145 complexes exhibited low cytotoxicity, and TMC-g-PEG-VAPG with relatively higher molecular weight of chitosan (50 kDa) could significantly enhance cellular uptake in SMCs.					
31336282	1	44	theme	smooth	237:242	arg1	SMCs					258:261	SMCs	258:261	SMCs	258:261	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	1	44	theme	smooth	237:242	arg1	cells					251:255	vascular smooth muscle cells	228:255	vascular smooth muscle cells (SMCs)	228:262	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	5	45	theme	muscle	1180:1185	arg1	actin					1187:1191	α-smooth muscle actin	1171:1191	α-smooth muscle actin	1171:1191	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	1	46	theme	muscle	244:249	arg1	SMCs					258:261	SMCs	258:261	SMCs	258:261	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	1	46	theme	muscle	244:249	arg1	cells					251:255	vascular smooth muscle cells	228:255	vascular smooth muscle cells (SMCs)	228:262	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	2	47	theme	excessive	441:449	arg1	proliferation					451:463	excessive proliferation	441:463	excessive proliferation	441:463	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	5	48	theme	actin	1187:1191	arg1	expression					1143:1152	the expression	1139:1152	the expression of myocardin and α-smooth muscle actin	1139:1191	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	7	49	theme	functional	1387:1396	arg1	membranes					1410:1418	The functional electrospun membranes	1383:1418	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes	1383:1464	The functional electrospun membranes containing TMC-g-PEG-VAPG/miRNA-145 complexes may have a great potential in the application of small-diameter blood vessel regeneration.					
31336282	5	50	theme	protein	1068:1074	arg1	levels					1076:1081	gene and protein levels	1059:1081	levels	1076:1081	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	4	51	from	uptake	861:866	arg1	SMCs					871:874	SMCs	871:874	SMCs	871:874	The TMC-g-PEG-VAPG/miRNA-145 complexes exhibited low cytotoxicity, and TMC-g-PEG-VAPG with relatively higher molecular weight of chitosan (50 kDa) could significantly enhance cellular uptake in SMCs.					
31336282	5	52	theme	α-smooth	1171:1178	arg1	actin					1187:1191	α-smooth muscle actin	1171:1191	α-smooth muscle actin	1171:1191	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	2	53	from	SMCs	383:386	arg1	phenotype					414:422	the normal contractile phenotype	391:422	the normal contractile phenotype	391:422	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	5	54	theme	poly	965:968	arg1	membranes					952:960	the electrospun membranes	936:960	the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone)	936:1021	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	5	54	theme	poly	965:968	arg1	complexes					925:933	TMC-g-PEG-VAPG/miRNA-145 complexes	900:933	TMC-g-PEG-VAPG/miRNA-145 complexes	900:933	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	5	55	with	loading	887:893	arg1	membranes					952:960	the electrospun membranes	936:960	the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone)	936:1021	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	5	55	with	loading	887:893	arg1	complexes					925:933	TMC-g-PEG-VAPG/miRNA-145 complexes	900:933	TMC-g-PEG-VAPG/miRNA-145 complexes	900:933	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	5	56	from	levels	1076:1081	arg1	day					1086:1088	day 3	1086:1090	day 3	1086:1090	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	5	56	from	levels	1076:1081	arg1	capable					1028:1034	capable	1028:1034	capable	1028:1034	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	3	57	theme	miRNA-145	627:635	arg1	target-delivery					608:622	target-delivery	608:622	target-delivery of miRNA-145 to SMCs	608:643	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	1	58	theme	main	143:146	arg1	challenges					148:157	the main challenges	139:157	the main challenges in small-diameter vascular regeneration	139:197	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	1	59	theme	cells	251:255	arg1	modulation					214:223	effective modulation	204:223	effective modulation of vascular smooth muscle cells (SMCs)	204:262	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
31336282	3	60	theme	proper	660:665	arg1	function					667:674	the proper function	656:674	the proper function	656:674	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	5	61	theme	controlling	1039:1049	arg1	SMCs					1051:1054	controlling SMCs	1039:1054	controlling SMCs	1039:1054	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	2	62	theme	microRNAs	320:328	arg1	miRNAs					331:336	miRNAs	331:336	miRNAs	331:336	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	2	62	theme	microRNAs	320:328	arg1	microRNAs					320:328	microRNAs	320:328	microRNAs (miRNAs) in vascular systems	320:357	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	2	62	theme	microRNAs	320:328	arg1	one					313:315	one	313:315	one	313:315	As one of microRNAs (miRNAs) in vascular systems, miRNA-145 can regulate SMCs in the normal contractile phenotype, and inhibit the excessive proliferation and intimal hyperplasia.					
31336282	6	63	from	phenotype	1320:1328	arg1	duration					1355:1362	the prolonged duration	1341:1362	the prolonged duration	1341:1362	Furthermore, miRNA-145 released from the electrospun membranes also showed in vitro bioactivity of modulating the contractile phenotype of SMCs in the prolonged duration, at least 56 days.					
31336282	0	64	theme	vascular	97:104	arg1	SMCs					106:109	target-regulating vascular SMCs	79:109	target-regulating vascular SMCs	79:109	Performance of TMC-g-PEG-VAPG/miRNA-145 complexes in electrospun membranes for target-regulating vascular SMCs.					
31336282	5	65	theme	SMCs	1051:1054	arg1	capable					1028:1034	capable	1028:1034	capable	1028:1034	Moreover, loading with TMC-g-PEG-VAPG/miRNA-145 complexes, the electrospun membranes of poly(ethylene glycol)-b-poly(L-lactide-co-ε-caprolactone) were capable of controlling SMCs at gene and protein levels on day 3 by targeting Krüppel-like factor 4 to increase the expression of myocardin and α-smooth muscle actin.					
31336282	3	66	theme	peptide-modified	503:518	arg1	chitosan-g-poly					530:544	VAPG peptide-modified trimethyl chitosan-g-poly	498:544	VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG)	498:578	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	3	66	theme	peptide-modified	503:518	arg1	glycol					555:560	ethylene glycol	546:560	ethylene glycol	546:560	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	3	66	theme	peptide-modified	503:518	arg1	TMC-g-PEG-VAPG					564:577	TMC-g-PEG-VAPG	564:577	TMC-g-PEG-VAPG	564:577	Herein, VAPG peptide-modified trimethyl chitosan-g-poly(ethylene glycol) (TMC-g-PEG-VAPG) was developed specially for target-delivery of miRNA-145 to SMCs to fulfill the proper function.					
31336282	0	67	theme	target-regulating	79:95	arg1	SMCs					106:109	target-regulating vascular SMCs	79:109	target-regulating vascular SMCs	79:109	Performance of TMC-g-PEG-VAPG/miRNA-145 complexes in electrospun membranes for target-regulating vascular SMCs.					
31336282	4	68	with	TMC-g-PEG-VAPG	748:761	arg1	weight					796:801	relatively higher molecular weight	768:801	relatively higher molecular weight of chitosan (50 kDa)	768:822	The TMC-g-PEG-VAPG/miRNA-145 complexes exhibited low cytotoxicity, and TMC-g-PEG-VAPG with relatively higher molecular weight of chitosan (50 kDa) could significantly enhance cellular uptake in SMCs.					
31336282	1	69	theme	small-diameter	162:175	arg1	regeneration					186:197	small-diameter vascular regeneration	162:197	small-diameter vascular regeneration	162:197	Restenosis is still one of the main challenges in small-diameter vascular regeneration, and effective modulation of vascular smooth muscle cells (SMCs) is essential to cope with the related issues.					
30382612	0	0	theme	Gastric	76:82	arg1	Glands					84:89	the Rat Fundic Gastric Glands	61:89	the Rat Fundic Gastric Glands	61:89	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.					
30382612	0	1	from	Content	25:31	arg1	Cells					52:56	Mucus Secreting Cells	36:56	Mucus Secreting Cells of the Rat Fundic Gastric Glands	36:89	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.					
30382612	4	2	used	used	462:465	arg2	We					459:460	We	459:460	We	459:460	We used several lectins combined with deglycosylation pretreatments to analyze the glycan composition of SMCs and MNCs.					
30382612	5	3	from	glycosylation	717:729	arg1	mucins					734:739	mucins	734:739	mucins	734:739	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	8	4	theme	endoplasmic	1186:1196	arg1	reticulum					1198:1206	the endoplasmic reticulum	1182:1206	the endoplasmic reticulum	1182:1206	This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus.					
30382612	3	5	from	secretion	437:445	arg1	general					450:456	general	450:456	general	450:456	Very little is known about the glycan composition of these mucins and of gastric secretion in general.					
30382612	9	6	theme	mucus	1279:1283	arg1	secretion					1285:1293	a mucus secretion	1277:1293	a mucus secretion with a different glycoconjugate composition	1277:1337	Our results indicate that SMCs and MNCs produce a mucus secretion with a different glycoconjugate composition.					
30382612	0	7	theme	Fundic	69:74	arg1	Glands					84:89	the Rat Fundic Gastric Glands	61:89	the Rat Fundic Gastric Glands	61:89	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.					
30382612	6	8	theme	mucins	822:827	arg1	expression					808:817	a different expression	796:817	a different expression of mucins in the SMCs	796:839	Our results also support previous reports that showed a different expression of mucins in the SMCs, depending on their superficial or deep location in the pit.					
30382612	3	9	theme	mucins	415:420	arg1	composition					394:404	the glycan composition	383:404	the glycan composition of these mucins	383:420	Very little is known about the glycan composition of these mucins and of gastric secretion in general.					
30382612	10	10	from	SMCs	1372:1375	arg1	varied					1362:1367	varied	1362:1367	varied	1362:1367	The secretion is more varied in SMCs.					
30382612	10	10	from	SMCs	1372:1375	arg1	secretion					1344:1352	The secretion	1340:1352	The secretion	1340:1352	The secretion is more varied in SMCs.					
30382612	1	11	theme	situated	269:276	arg1	MNCs					263:266	MNCs	263:266	MNCs	263:266	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	11	theme	situated	269:276	arg1	cells					256:260	mucous neck cells	244:260	mucous neck cells (MNCs) situated in the neck of the glands	244:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	11	12	from	differences	1527:1537	arg1	glycosylation					1542:1554	glycosylation	1542:1554	glycosylation observed in each cell type	1542:1581	As our results coincide with what we know about glycosylation of mucins, we can conclude that most of the glycans detected belong to mucins, and the differences in glycosylation observed in each cell type may be due, mainly, to the different secreted mucins.					
30382612	4	13	theme	MNCs	573:576	arg1	composition					549:559	the glycan composition	538:559	the glycan composition of SMCs and MNCs	538:576	We used several lectins combined with deglycosylation pretreatments to analyze the glycan composition of SMCs and MNCs.					
30382612	4	14	theme	deglycosylation	497:511	arg1	pretreatments					513:525	deglycosylation pretreatments	497:525	deglycosylation pretreatments	497:525	We used several lectins combined with deglycosylation pretreatments to analyze the glycan composition of SMCs and MNCs.					
30382612	0	15	theme	Glands	84:89	arg1	Cells					52:56	Mucus Secreting Cells	36:56	Mucus Secreting Cells of the Rat Fundic Gastric Glands	36:89	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.					
30382612	1	16	theme	stomach	218:224	arg1	surface					203:209	the surface	199:209	the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands	199:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	5	17	theme	Gal	651:653	arg1	consistent					676:685	consistent	676:685	consistent	676:685	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	5	17	theme	Gal	651:653	arg1	presence					602:609	the presence	598:609	the presence	598:609	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	6	18	theme	different	798:806	arg1	expression					808:817	a different expression	796:817	a different expression of mucins in the SMCs	796:839	Our results also support previous reports that showed a different expression of mucins in the SMCs, depending on their superficial or deep location in the pit.					
30382612	3	19	theme	gastric	429:435	arg1	secretion					437:445	gastric secretion	429:445	gastric secretion in general	429:456	Very little is known about the glycan composition of these mucins and of gastric secretion in general.					
30382612	9	20	theme	different	1302:1310	arg1	composition					1327:1337	a different glycoconjugate composition	1300:1337	a different glycoconjugate composition	1300:1337	Our results indicate that SMCs and MNCs produce a mucus secretion with a different glycoconjugate composition.					
30382612	1	21	theme	mucous	146:151	arg1	cells					153:157	mucous cells	146:157	mucous cells	146:157	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	4	22	theme	several	467:473	arg1	lectins					475:481	several lectins	467:481	several lectins combined with deglycosylation pretreatments	467:525	We used several lectins combined with deglycosylation pretreatments to analyze the glycan composition of SMCs and MNCs.					
30382612	6	23	from	location	881:888	arg1	pit					897:899	the pit	893:899	the pit	893:899	Our results also support previous reports that showed a different expression of mucins in the SMCs, depending on their superficial or deep location in the pit.					
30382612	8	24	theme	Golgi	1212:1216	arg1	apparatus					1218:1226	Golgi apparatus	1212:1226	Golgi apparatus	1212:1226	This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus.					
30382612	11	25	located	observed	1556:1563	arg1	type					1578:1581	each cell type	1568:1581	each cell type	1568:1581	As our results coincide with what we know about glycosylation of mucins, we can conclude that most of the glycans detected belong to mucins, and the differences in glycosylation observed in each cell type may be due, mainly, to the different secreted mucins.					
30382612	11	25	located	observed	1556:1563	arg2	glycosylation					1542:1554	glycosylation	1542:1554	glycosylation observed in each cell type	1542:1581	As our results coincide with what we know about glycosylation of mucins, we can conclude that most of the glycans detected belong to mucins, and the differences in glycosylation observed in each cell type may be due, mainly, to the different secreted mucins.					
30382612	1	26	theme	cells	153:157	arg1	types					137:141	two types	133:141	two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands	133:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	0	27	theme	Glycoconjugate	10:23	arg1	Content					25:31	Different Glycoconjugate Content	0:31	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.	0:90	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.					
30382612	1	28	contain	contain	125:131	arg1	glands					103:108	The fundic glands	92:108	The fundic glands of the stomach	92:123	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	28	contain	contain	125:131	arg2	types					137:141	two types	133:141	two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands	133:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	11	29	gly	glycosylation	1426:1438	arg1	mucins					1443:1448	mucins	1443:1448	mucins	1443:1448	As our results coincide with what we know about glycosylation of mucins, we can conclude that most of the glycans detected belong to mucins, and the differences in glycosylation observed in each cell type may be due, mainly, to the different secreted mucins.					
30382612	1	30	dep	types	137:141	arg1	cells					175:179	surface mucous cells	160:179	two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands	133:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	30	dep	types	137:141	arg1	SMCs					182:185	SMCs	182:185	SMCs	182:185	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	13	31	dep	©	1668:1668	arg1	Inc.					1694:1697	Inc.	1694:1697	Inc.	1694:1697	© 2018 Wiley Periodicals, Inc.					
30382612	5	32	theme	sialic	623:628	arg1	acid					630:633	terminal sialic acid	614:633	terminal sialic acid	614:633	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	0	33	theme	Different	0:8	arg1	Content					25:31	Different Glycoconjugate Content	0:31	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.	0:90	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.					
30382612	6	34	theme	deep	876:879	arg1	location					881:888	their superficial or deep location	855:888	their superficial or deep location in the pit	855:899	Our results also support previous reports that showed a different expression of mucins in the SMCs, depending on their superficial or deep location in the pit.					
30382612	1	35	theme	surface	160:166	arg1	cells					175:179	surface mucous cells	160:179	two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands	133:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	35	theme	surface	160:166	arg1	SMCs					182:185	SMCs	182:185	SMCs	182:185	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	8	36	theme	labeling	1067:1074	arg1	residues					1082:1089	labeling sugar residues	1067:1089	labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus	1067:1226	This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus.					
30382612	8	36	theme	labeling	1067:1074	arg1	lectins					1055:1061	the lectins	1051:1061	the lectins	1051:1061	This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus.					
30382612	11	37	theme	secreted	1620:1627	arg1	mucins					1629:1634	the different secreted mucins	1606:1634	the different secreted mucins	1606:1634	As our results coincide with what we know about glycosylation of mucins, we can conclude that most of the glycans detected belong to mucins, and the differences in glycosylation observed in each cell type may be due, mainly, to the different secreted mucins.					
30382612	6	38	theme	superficial	861:871	arg1	location					881:888	their superficial or deep location	855:888	their superficial or deep location in the pit	855:899	Our results also support previous reports that showed a different expression of mucins in the SMCs, depending on their superficial or deep location in the pit.					
30382612	1	39	theme	mucous	168:173	arg1	cells					175:179	surface mucous cells	160:179	two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands	133:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	39	theme	mucous	168:173	arg1	SMCs					182:185	SMCs	182:185	SMCs	182:185	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	2	40	theme	glycosylated	333:344	arg1	mucins					318:323	mucins	318:323	mucins	318:323	They produce mucins, highly glycosylated proteins.					
30382612	2	40	theme	glycosylated	333:344	arg1	proteins					346:353	highly glycosylated proteins	326:353	highly glycosylated proteins	326:353	They produce mucins, highly glycosylated proteins.					
30382612	1	41	theme	pits	234:237	arg1	surface					203:209	the surface	199:209	the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands	199:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	5	42	theme	terminal	614:621	arg1	acid					630:633	terminal sialic acid	614:633	terminal sialic acid	614:633	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	1	43	theme	fundic	96:101	arg1	glands					103:108	The fundic glands	92:108	The fundic glands of the stomach	92:123	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	4	44	theme	glycan	542:547	arg1	composition					549:559	the glycan composition	538:559	the glycan composition of SMCs and MNCs	538:576	We used several lectins combined with deglycosylation pretreatments to analyze the glycan composition of SMCs and MNCs.					
30382612	0	45	theme	Secreting	42:50	arg1	Cells					52:56	Mucus Secreting Cells	36:56	Mucus Secreting Cells of the Rat Fundic Gastric Glands	36:89	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.					
30382612	12	46	dep	2018	1662:1665	arg1	301:2128-2144					1647:1659	301:2128-2144	1647:1659	301:2128-2144	1647:1659	Anat Rec, 301:2128-2144, 2018.					
30382612	12	46	dep	2018	1662:1665	arg1	Rec					1642:1644	Anat Rec	1637:1644	Anat Rec	1637:1644	Anat Rec, 301:2128-2144, 2018.					
30382612	1	47	from	surface	203:209	arg1	located					188:194	located	188:194	located	188:194	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	48	theme	glands	297:302	arg1	neck					285:288	the neck	281:288	the neck of the glands	281:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	5	49	gly	glycosylation	717:729	arg1	mucins					734:739	mucins	734:739	mucins	734:739	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	9	50	theme	glycoconjugate	1312:1325	arg1	composition					1327:1337	a different glycoconjugate composition	1300:1337	a different glycoconjugate composition	1300:1337	Our results indicate that SMCs and MNCs produce a mucus secretion with a different glycoconjugate composition.					
30382612	4	51	theme	SMCs	564:567	arg1	composition					549:559	the glycan composition	538:559	the glycan composition of SMCs and MNCs	538:576	We used several lectins combined with deglycosylation pretreatments to analyze the glycan composition of SMCs and MNCs.					
30382612	0	52	theme	Mucus	36:40	arg1	Cells					52:56	Mucus Secreting Cells	36:56	Mucus Secreting Cells of the Rat Fundic Gastric Glands	36:89	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.					
30382612	1	53	from	situated	269:276	arg1	neck					285:288	the neck	281:288	the neck of the glands	281:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	6	54	theme	previous	767:774	arg1	reports					776:782	previous reports	767:782	previous reports that showed a different expression of mucins in the SMCs	767:839	Our results also support previous reports that showed a different expression of mucins in the SMCs, depending on their superficial or deep location in the pit.					
30382612	11	55	theme	different	1610:1618	arg1	mucins					1629:1634	the different secreted mucins	1606:1634	the different secreted mucins	1606:1634	As our results coincide with what we know about glycosylation of mucins, we can conclude that most of the glycans detected belong to mucins, and the differences in glycosylation observed in each cell type may be due, mainly, to the different secreted mucins.					
30382612	5	56	theme	previous	692:699	arg1	knowledge					701:709	previous knowledge	692:709	previous knowledge about glycosylation in mucins	692:739	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	1	57	theme	mucous	244:249	arg1	MNCs					263:266	MNCs	263:266	MNCs	263:266	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	57	theme	mucous	244:249	arg1	cells					256:260	mucous neck cells	244:260	mucous neck cells (MNCs) situated in the neck of the glands	244:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	7	58	theme	perinuclear	932:942	arg1	region					944:949	the perinuclear region	928:949	the perinuclear region of the SMCs	928:961	Some lectins labeled only the perinuclear region of the SMCs, but not the apical region, where the secretory granules are stored.					
30382612	9	59	with	secretion	1285:1293	arg1	composition					1327:1337	a different glycoconjugate composition	1300:1337	a different glycoconjugate composition	1300:1337	Our results indicate that SMCs and MNCs produce a mucus secretion with a different glycoconjugate composition.					
30382612	8	60	theme	synthesis	1155:1163	arg1	steps					1139:1143	the first steps	1129:1143	the first steps	1129:1143	This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus.					
30382612	5	61	theme	acid	630:633	arg1	consistent					676:685	consistent	676:685	consistent	676:685	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	5	61	theme	acid	630:633	arg1	presence					602:609	the presence	598:609	the presence	598:609	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	1	62	theme	neck	251:254	arg1	MNCs					263:266	MNCs	263:266	MNCs	263:266	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	62	theme	neck	251:254	arg1	cells					256:260	mucous neck cells	244:260	mucous neck cells (MNCs) situated in the neck of the glands	244:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	7	63	theme	SMCs	958:961	arg1	region					944:949	the perinuclear region	928:949	the perinuclear region of the SMCs	928:961	Some lectins labeled only the perinuclear region of the SMCs, but not the apical region, where the secretory granules are stored.					
30382612	7	64	theme	apical	976:981	arg1	region					983:988	the apical region	972:988	the apical region	972:988	Some lectins labeled only the perinuclear region of the SMCs, but not the apical region, where the secretory granules are stored.					
30382612	8	65	theme	sugar	1076:1080	arg1	residues					1082:1089	labeling sugar residues	1067:1089	labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus	1067:1226	This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus.					
30382612	8	65	theme	sugar	1076:1080	arg1	lectins					1055:1061	the lectins	1051:1061	the lectins	1051:1061	This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus.					
30382612	8	66	theme	glycan	1148:1153	arg1	synthesis					1155:1163	glycan synthesis	1148:1163	glycan synthesis	1148:1163	This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus.					
30382612	1	67	theme	cells	256:260	arg1	surface					203:209	the surface	199:209	the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands	199:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	5	68	with	consistent	676:685	arg1	knowledge					701:709	previous knowledge	692:709	previous knowledge about glycosylation in mucins	692:739	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30382612	6	69	from	expression	808:817	arg1	SMCs					836:839	the SMCs	832:839	the SMCs	832:839	Our results also support previous reports that showed a different expression of mucins in the SMCs, depending on their superficial or deep location in the pit.					
30382612	11	70	theme	mucins	1443:1448	arg1	glycosylation					1426:1438	glycosylation	1426:1438	glycosylation of mucins	1426:1448	As our results coincide with what we know about glycosylation of mucins, we can conclude that most of the glycans detected belong to mucins, and the differences in glycosylation observed in each cell type may be due, mainly, to the different secreted mucins.					
30382612	12	71	theme	Anat	1637:1640	arg1	301:2128-2144					1647:1659	301:2128-2144	1647:1659	301:2128-2144	1647:1659	Anat Rec, 301:2128-2144, 2018.					
30382612	12	71	theme	Anat	1637:1640	arg1	Rec					1642:1644	Anat Rec	1637:1644	Anat Rec	1637:1644	Anat Rec, 301:2128-2144, 2018.					
30382612	1	72	theme	stomach	117:123	arg1	glands					103:108	The fundic glands	92:108	The fundic glands of the stomach	92:123	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	73	theme	located	188:194	arg1	cells					175:179	surface mucous cells	160:179	two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands	133:302	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	1	73	theme	located	188:194	arg1	SMCs					182:185	SMCs	182:185	SMCs	182:185	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	0	74	theme	Rat	65:67	arg1	Glands					84:89	the Rat Fundic Gastric Glands	61:89	the Rat Fundic Gastric Glands	61:89	Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands.					
30382612	2	75	gly	glycosylated	333:344	arg1	mucins					318:323	mucins	318:323	mucins	318:323	They produce mucins, highly glycosylated proteins.					
30382612	2	75	gly	glycosylated	333:344	arg1	proteins					346:353	highly glycosylated proteins	326:353	highly glycosylated proteins	326:353	They produce mucins, highly glycosylated proteins.					
30382612	8	76	theme	first	1133:1137	arg1	steps					1139:1143	the first steps	1129:1143	the first steps	1129:1143	This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus.					
30382612	3	77	theme	glycan	387:392	arg1	composition					394:404	the glycan composition	383:404	the glycan composition of these mucins	383:420	Very little is known about the glycan composition of these mucins and of gastric secretion in general.					
30382612	11	78	theme	cell	1573:1576	arg1	type					1578:1581	each cell type	1568:1581	each cell type	1568:1581	As our results coincide with what we know about glycosylation of mucins, we can conclude that most of the glycans detected belong to mucins, and the differences in glycosylation observed in each cell type may be due, mainly, to the different secreted mucins.					
30382612	7	79	theme	secretory	1001:1009	arg1	granules					1011:1018	the secretory granules	997:1018	the secretory granules	997:1018	Some lectins labeled only the perinuclear region of the SMCs, but not the apical region, where the secretory granules are stored.					
30382612	10	80	from	varied	1362:1367	arg1	SMCs					1372:1375	SMCs	1372:1375	SMCs	1372:1375	The secretion is more varied in SMCs.					
30382612	1	81	from	neck	285:288	arg1	situated					269:276	situated	269:276	situated	269:276	The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands.					
30382612	5	82	theme	subterminal	639:649	arg1	Gal					651:653	subterminal Gal	639:653	subterminal Gal	639:653	The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins.					
30035760	0	0	theme	Biophysical	92:102	arg1	Techniques					104:113	Biophysical Techniques	92:113	Biophysical Techniques	92:113	Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques.					
30035760	6	1	theme	composition	942:952	arg1	effect					916:921	the effect	912:921	the effect of glycan size and composition on ligand binding	912:970	These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.					
30035760	10	2	theme	therapeutics	1661:1672	arg1	action					1651:1656	action	1651:1656	action of therapeutics	1651:1672	The experimental protocols discussed here hold promise for the characterization of mammalian glycoproteins to give insight into their function and investigate the mechanism of action of therapeutics.					
30035760	3	3	theme	B	501:501	arg1	CD22					517:520	human B cell receptor CD22	495:520	human B cell receptor CD22	495:520	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	8	4	theme	due	1217:1219	arg1	crystallization					1201:1215	crystallization	1201:1215	crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment	1201:1312	Recombinant glycoproteins produced in HEK293S cells are amenable to crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment.					
30035760	5	5	theme	suspension	668:677	arg1	HEK					703:705	HEK	703:705	HEK	703:705	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	5	5	theme	suspension	668:677	arg1	kidney					695:700	suspension human embryonic kidney	668:700	Two suspension human embryonic kidney (HEK) cell lines	664:717	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	3	6	theme	cell	503:506	arg1	CD22					517:520	human B cell receptor CD22	495:520	human B cell receptor CD22	495:520	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	10	7	theme	mammalian	1558:1566	arg1	glycoproteins					1568:1580	mammalian glycoproteins	1558:1580	mammalian glycoproteins	1558:1580	The experimental protocols discussed here hold promise for the characterization of mammalian glycoproteins to give insight into their function and investigate the mechanism of action of therapeutics.					
30035760	6	8	from	effect	916:921	arg1	binding					964:970	ligand binding	957:970	ligand binding	957:970	These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.					
30035760	6	9	theme	size	933:936	arg1	effect					916:921	the effect	912:921	the effect of glycan size and composition on ligand binding	912:970	These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.					
30035760	5	10	theme	human	679:683	arg1	HEK					703:705	HEK	703:705	HEK	703:705	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	5	10	theme	human	679:683	arg1	kidney					695:700	suspension human embryonic kidney	668:700	Two suspension human embryonic kidney (HEK) cell lines	664:717	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	10	11	theme	experimental	1479:1490	arg1	protocols					1492:1500	The experimental protocols	1475:1500	The experimental protocols discussed here	1475:1515	The experimental protocols discussed here hold promise for the characterization of mammalian glycoproteins to give insight into their function and investigate the mechanism of action of therapeutics.					
30035760	9	12	theme	small	1389:1393	arg1	molecules					1395:1403	small molecules	1389:1403	small molecules	1389:1403	We present methods for soaking glycoprotein crystals with heavy atoms and small molecules for phase determination and analysis of ligand binding, respectively.					
30035760	8	13	theme	glycan	1224:1229	arg1	homogeneity					1231:1241	glycan homogeneity	1224:1241	glycan homogeneity	1224:1241	Recombinant glycoproteins produced in HEK293S cells are amenable to crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment.					
30035760	6	14	theme	glycan	926:931	arg1	size					933:936	glycan size	926:936	glycan size	926:936	These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.					
30035760	5	15	theme	embryonic	685:693	arg1	HEK					703:705	HEK	703:705	HEK	703:705	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	5	15	theme	embryonic	685:693	arg1	kidney					695:700	suspension human embryonic kidney	668:700	Two suspension human embryonic kidney (HEK) cell lines	664:717	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	4	16	gly	glycoprotein	616:627	arg1	glycoprotein					616:627	mammalian glycoprotein ectodomains	606:639	mammalian glycoprotein ectodomains containing Ig domains	606:661	We propose that these approaches are broadly applicable to the characterization of mammalian glycoprotein ectodomains containing Ig domains.					
30035760	6	17	gly	glycoproteins	852:864	arg1	glycoproteins					852:864	These recombinant glycoproteins	834:864	These recombinant glycoproteins with different glycoforms	834:890	These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.					
30035760	3	18	theme	domain	485:490	arg1	expression					420:429	expression	420:429	expression	420:429	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	3	18	theme	domain	485:490	arg1	characterization					447:462	biophysical characterization	435:462	biophysical characterization	435:462	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	3	18	theme	domain	485:490	arg1	design					412:417	design	412:417	design	412:417	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	2	19	gly	glycoproteins	274:286	arg1	glycoproteins					274:286	these glycoproteins	268:286	these glycoproteins	268:286	On leukocytes, several of these glycoproteins possess immunoglobulin (Ig) folds and are central to immune recognition and regulation.					
30035760	5	20	gly	glycoproteins	761:773	arg1	glycoproteins					761:773	glycoproteins	761:773	glycoproteins harbouring complex and high-mannose glycans	761:817	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	8	21	gly	glycoproteins	1145:1157	arg1	glycoproteins					1145:1157	Recombinant glycoproteins	1133:1157	Recombinant glycoproteins produced in HEK293S cells	1133:1183	Recombinant glycoproteins produced in HEK293S cells are amenable to crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment.					
30035760	1	22	theme	cellular	177:184	arg1	function					186:193	cellular function	177:193	cellular function	177:193	Glycoproteins on the surface of cells play critical roles in cellular function, including signalling, adhesion and transport.					
30035760	9	23	theme	phase	1409:1413	arg1	determination					1415:1427	phase determination	1409:1427	phase determination	1409:1427	We present methods for soaking glycoprotein crystals with heavy atoms and small molecules for phase determination and analysis of ligand binding, respectively.					
30035760	8	24	theme	H	1302:1302	arg1	treatment					1304:1312	endoglycosidase H treatment	1286:1312	endoglycosidase H treatment	1286:1312	Recombinant glycoproteins produced in HEK293S cells are amenable to crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment.					
30035760	2	25	theme	glycoproteins	274:286	arg1	several					257:263	several	257:263	several	257:263	On leukocytes, several of these glycoproteins possess immunoglobulin (Ig) folds and are central to immune recognition and regulation.					
30035760	8	26	theme	reduced	1244:1250	arg1	flexibility					1252:1262	reduced flexibility	1244:1262	reduced flexibility	1244:1262	Recombinant glycoproteins produced in HEK293S cells are amenable to crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment.					
30035760	10	27	theme	action	1651:1656	arg1	mechanism					1638:1646	the mechanism	1634:1646	the mechanism of action of therapeutics	1634:1672	The experimental protocols discussed here hold promise for the characterization of mammalian glycoproteins to give insight into their function and investigate the mechanism of action of therapeutics.					
30035760	5	28	used	used	745:748	arg2	HEK293F					720:726	HEK293F	720:726	HEK293F	720:726	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	5	28	used	used	745:748	arg2	lines					713:717	Two suspension human embryonic kidney (HEK) cell lines	664:717	Two suspension human embryonic kidney (HEK) cell lines	664:717	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	5	28	used	used	745:748	arg2	HEK293S					732:738	HEK293S	732:738	HEK293S	732:738	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	2	29	contain	possess	288:294	arg1	several					257:263	several	257:263	several	257:263	On leukocytes, several of these glycoproteins possess immunoglobulin (Ig) folds and are central to immune recognition and regulation.					
30035760	2	29	contain	possess	288:294	arg2	Ig					312:313	Ig	312:313	Ig	312:313	On leukocytes, several of these glycoproteins possess immunoglobulin (Ig) folds and are central to immune recognition and regulation.					
30035760	2	29	contain	possess	288:294	arg2	immunoglobulin					296:309	immunoglobulin	296:309	immunoglobulin (Ig) folds	296:320	On leukocytes, several of these glycoproteins possess immunoglobulin (Ig) folds and are central to immune recognition and regulation.					
30035760	5	30	theme	cell	708:711	arg1	lines					713:717	Two suspension human embryonic kidney (HEK) cell lines	664:717	Two suspension human embryonic kidney (HEK) cell lines	664:717	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	5	30	theme	cell	708:711	arg1	HEK293S					732:738	HEK293S	732:738	HEK293S	732:738	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	5	30	theme	cell	708:711	arg1	HEK293F					720:726	HEK293F	720:726	HEK293F	720:726	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	7	31	dep	kinetics	1011:1018	arg1	the					1007:1009	the	1007:1009	the	1007:1009	We discuss protocols for studying the kinetics and thermodynamics of glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates.					
30035760	4	32	theme	Ig	652:653	arg1	domains					655:661	Ig domains	652:661	Ig domains	652:661	We propose that these approaches are broadly applicable to the characterization of mammalian glycoprotein ectodomains containing Ig domains.					
30035760	0	33	theme	Glycoproteins	20:32	arg1	Characterization					0:15	Characterization	0:15	Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques.	0:114	Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques.					
30035760	8	34	theme	Recombinant	1133:1143	arg1	glycoproteins					1145:1157	Recombinant glycoproteins	1133:1157	Recombinant glycoproteins produced in HEK293S cells	1133:1183	Recombinant glycoproteins produced in HEK293S cells are amenable to crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment.					
30035760	2	35	dep	immunoglobulin	296:309	arg1	folds					316:320	folds	316:320	folds	316:320	On leukocytes, several of these glycoproteins possess immunoglobulin (Ig) folds and are central to immune recognition and regulation.					
30035760	0	36	theme	Immunoglobulin	43:56	arg1	Fold					58:61	the Immunoglobulin Fold	39:61	the Immunoglobulin Fold	39:61	Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques.					
30035760	9	37	theme	ligand	1445:1450	arg1	binding					1452:1458	ligand binding	1445:1458	ligand binding	1445:1458	We present methods for soaking glycoprotein crystals with heavy atoms and small molecules for phase determination and analysis of ligand binding, respectively.					
30035760	7	38	theme	therapeutic	1100:1110	arg1	candidates					1121:1130	therapeutic antibody candidates	1100:1130	therapeutic antibody candidates	1100:1130	We discuss protocols for studying the kinetics and thermodynamics of glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates.					
30035760	3	39	theme	biophysical	435:445	arg1	characterization					447:462	biophysical characterization	435:462	biophysical characterization	435:462	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	5	40	theme	complex	786:792	arg1	glycans					811:817	complex and high-mannose glycans	786:817	glycans	811:817	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	9	41	theme	binding	1452:1458	arg1	analysis					1433:1440	analysis	1433:1440	analysis of ligand binding	1433:1458	We present methods for soaking glycoprotein crystals with heavy atoms and small molecules for phase determination and analysis of ligand binding, respectively.					
30035760	9	41	theme	binding	1452:1458	arg1	determination					1415:1427	phase determination	1409:1427	phase determination	1409:1427	We present methods for soaking glycoprotein crystals with heavy atoms and small molecules for phase determination and analysis of ligand binding, respectively.					
30035760	7	42	theme	antibody	1112:1119	arg1	candidates					1121:1130	therapeutic antibody candidates	1100:1130	therapeutic antibody candidates	1100:1130	We discuss protocols for studying the kinetics and thermodynamics of glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates.					
30035760	4	43	contain	containing	641:650	arg2	domains					655:661	Ig domains	652:661	Ig domains	652:661	We propose that these approaches are broadly applicable to the characterization of mammalian glycoprotein ectodomains containing Ig domains.					
30035760	4	43	contain	containing	641:650	arg1	ectodomains					629:639	mammalian glycoprotein ectodomains	606:639	mammalian glycoprotein ectodomains containing Ig domains	606:661	We propose that these approaches are broadly applicable to the characterization of mammalian glycoprotein ectodomains containing Ig domains.					
30035760	7	44	theme	binding	1055:1061	arg1	thermodynamics					1024:1037	thermodynamics	1024:1037	thermodynamics	1024:1037	We discuss protocols for studying the kinetics and thermodynamics of glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates.					
30035760	7	44	theme	binding	1055:1061	arg1	kinetics					1011:1018	kinetics	1011:1018	kinetics	1011:1018	We discuss protocols for studying the kinetics and thermodynamics of glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates.					
30035760	6	45	with	glycoproteins	852:864	arg1	glycoforms					881:890	different glycoforms	871:890	different glycoforms	871:890	These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.					
30035760	5	46	theme	kidney	695:700	arg1	lines					713:717	Two suspension human embryonic kidney (HEK) cell lines	664:717	Two suspension human embryonic kidney (HEK) cell lines	664:717	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	5	46	theme	kidney	695:700	arg1	HEK293S					732:738	HEK293S	732:738	HEK293S	732:738	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	5	46	theme	kidney	695:700	arg1	HEK293F					720:726	HEK293F	720:726	HEK293F	720:726	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	6	47	theme	different	871:879	arg1	glycoforms					881:890	different glycoforms	871:890	different glycoforms	871:890	These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.					
30035760	0	48	with	Glycoproteins	20:32	arg1	Fold					58:61	the Immunoglobulin Fold	39:61	the Immunoglobulin Fold	39:61	Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques.					
30035760	2	49	theme	immune	341:346	arg1	recognition					348:358	recognition	348:358	recognition	348:358	On leukocytes, several of these glycoproteins possess immunoglobulin (Ig) folds and are central to immune recognition and regulation.					
30035760	5	50	theme	high-mannose	798:809	arg1	glycans					811:817	complex and high-mannose glycans	786:817	glycans	811:817	Two suspension human embryonic kidney (HEK) cell lines, HEK293F and HEK293S, are used to express glycoproteins harbouring complex and high-mannose glycans, respectively.					
30035760	7	51	gly	glycoprotein	1042:1053	arg1	glycoprotein					1042:1053	glycoprotein binding	1042:1061	glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates	1042:1130	We discuss protocols for studying the kinetics and thermodynamics of glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates.					
30035760	8	52	theme	HEK293S	1171:1177	arg1	cells					1179:1183	HEK293S cells	1171:1183	HEK293S cells	1171:1183	Recombinant glycoproteins produced in HEK293S cells are amenable to crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment.					
30035760	1	53	theme	critical	159:166	arg1	roles					168:172	critical roles	159:172	critical roles	159:172	Glycoproteins on the surface of cells play critical roles in cellular function, including signalling, adhesion and transport.					
30035760	3	54	theme	human	495:499	arg1	CD22					517:520	human B cell receptor CD22	495:520	human B cell receptor CD22	495:520	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	1	55	from	Glycoproteins	116:128	arg1	surface					137:143	the surface	133:143	the surface of cells	133:152	Glycoproteins on the surface of cells play critical roles in cellular function, including signalling, adhesion and transport.					
30035760	6	56	theme	ligand	957:962	arg1	binding					964:970	ligand binding	957:970	ligand binding	957:970	These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.					
30035760	9	57	theme	glycoprotein	1346:1357	arg1	crystals					1359:1366	glycoprotein crystals	1346:1366	glycoprotein crystals	1346:1366	We present methods for soaking glycoprotein crystals with heavy atoms and small molecules for phase determination and analysis of ligand binding, respectively.					
30035760	7	58	theme	glycoprotein	1042:1053	arg1	binding					1055:1061	glycoprotein binding	1042:1061	glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates	1042:1130	We discuss protocols for studying the kinetics and thermodynamics of glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates.					
30035760	3	59	theme	extracellular	471:483	arg1	domain					485:490	the extracellular domain	467:490	the extracellular domain of human B cell receptor CD22	467:520	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	3	59	theme	extracellular	471:483	arg1	CD22					517:520	human B cell receptor CD22	495:520	human B cell receptor CD22	495:520	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	4	60	theme	mammalian	606:614	arg1	ectodomains					629:639	mammalian glycoprotein ectodomains	606:639	mammalian glycoprotein ectodomains containing Ig domains	606:661	We propose that these approaches are broadly applicable to the characterization of mammalian glycoprotein ectodomains containing Ig domains.					
30035760	7	61	theme	relevant	1079:1086	arg1	ligands					1088:1094	biologically relevant ligands	1066:1094	biologically relevant ligands	1066:1094	We discuss protocols for studying the kinetics and thermodynamics of glycoprotein binding to biologically relevant ligands and therapeutic antibody candidates.					
30035760	6	62	theme	recombinant	840:850	arg1	glycoproteins					852:864	These recombinant glycoproteins	834:864	These recombinant glycoproteins with different glycoforms	834:890	These recombinant glycoproteins with different glycoforms allow investigating the effect of glycan size and composition on ligand binding.					
30035760	1	63	theme	cells	148:152	arg1	surface					137:143	the surface	133:143	the surface of cells	133:152	Glycoproteins on the surface of cells play critical roles in cellular function, including signalling, adhesion and transport.					
30035760	10	64	gly	glycoproteins	1568:1580	arg1	glycoproteins					1568:1580	mammalian glycoproteins	1558:1580	mammalian glycoproteins	1558:1580	The experimental protocols discussed here hold promise for the characterization of mammalian glycoproteins to give insight into their function and investigate the mechanism of action of therapeutics.					
30035760	8	65	theme	endoglycosidase	1286:1300	arg1	treatment					1304:1312	endoglycosidase H treatment	1286:1312	endoglycosidase H treatment	1286:1312	Recombinant glycoproteins produced in HEK293S cells are amenable to crystallization due to glycan homogeneity, reduced flexibility and susceptibility to endoglycosidase H treatment.					
30035760	10	66	theme	glycoproteins	1568:1580	arg1	characterization					1538:1553	the characterization	1534:1553	the characterization of mammalian glycoproteins	1534:1580	The experimental protocols discussed here hold promise for the characterization of mammalian glycoproteins to give insight into their function and investigate the mechanism of action of therapeutics.					
30035760	9	67	gly	glycoprotein	1346:1357	arg1	glycoprotein					1346:1357	glycoprotein crystals	1346:1366	glycoprotein crystals	1346:1366	We present methods for soaking glycoprotein crystals with heavy atoms and small molecules for phase determination and analysis of ligand binding, respectively.					
30035760	3	68	theme	receptor	508:515	arg1	CD22					517:520	human B cell receptor CD22	495:520	human B cell receptor CD22	495:520	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	4	69	theme	ectodomains	629:639	arg1	characterization					586:601	the characterization	582:601	the characterization of mammalian glycoprotein ectodomains containing Ig domains	582:661	We propose that these approaches are broadly applicable to the characterization of mammalian glycoprotein ectodomains containing Ig domains.					
30035760	0	70	theme	X-Ray	66:70	arg1	Crystallography					72:86	X-Ray Crystallography	66:86	X-Ray Crystallography	66:86	Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques.					
30035760	9	71	theme	heavy	1373:1377	arg1	atoms					1379:1383	heavy atoms	1373:1383	heavy atoms	1373:1383	We present methods for soaking glycoprotein crystals with heavy atoms and small molecules for phase determination and analysis of ligand binding, respectively.					
30035760	3	72	theme	CD22	517:520	arg1	domain					485:490	the extracellular domain	467:490	the extracellular domain of human B cell receptor CD22	467:520	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	3	72	theme	CD22	517:520	arg1	CD22					517:520	human B cell receptor CD22	495:520	human B cell receptor CD22	495:520	Here, we present a platform for the design, expression and biophysical characterization of the extracellular domain of human B cell receptor CD22.					
30035760	4	73	theme	glycoprotein	616:627	arg1	ectodomains					629:639	mammalian glycoprotein ectodomains	606:639	mammalian glycoprotein ectodomains containing Ig domains	606:661	We propose that these approaches are broadly applicable to the characterization of mammalian glycoprotein ectodomains containing Ig domains.					
29215790	6	0	theme	strong	883:888	arg1	reactivity					890:899	strong reactivity	883:899	strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines	883:976	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	11	1	theme	tumor	2038:2042	arg1	progression					2044:2054	tumor progression	2038:2054	tumor progression	2038:2054	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	7	2	theme	human	1150:1154	arg1	transcriptase					1175:1187	human telomerase reverse transcriptase	1150:1187	human telomerase reverse transcriptase	1150:1187	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	4	3	theme	fucosylated	573:583	arg1	structures					585:594	the fucosylated structures	569:594	the fucosylated structures	569:594	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	3	theme	fucosylated	573:583	arg1	sLeX/A					607:612	sLeX/A	607:612	sLeX/A	607:612	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	10	4	theme	protein	1826:1832	arg1	kinase					1834:1839	p38 mitogen-activated protein kinase	1804:1839	p38 mitogen-activated protein kinase signaling pathways	1804:1858	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	10	5	theme	growth	1668:1673	arg1	factor					1675:1680	vascular endothelial growth factor	1647:1680	vascular endothelial growth factor	1647:1680	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	10	6	theme	vascular	1647:1654	arg1	factor					1675:1680	vascular endothelial growth factor	1647:1680	vascular endothelial growth factor	1647:1680	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	5	7	theme	primary	832:838	arg1	cultures					845:852	established primary cell cultures	820:852	established primary cell cultures	820:852	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	4	8	theme	cell	654:657	arg1	proliferation					659:671	cell proliferation	654:671	cell proliferation in invasive ductal carcinomas (IDC)	654:707	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	10	9	theme	transforming	1687:1698	arg1	beta					1714:1717	transforming growth factor beta	1687:1717	transforming growth factor beta	1687:1717	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	1	10	theme	fucosylated	191:201	arg1	X/A					233:235	sialyl-Lewis X/A	220:235	sialyl-Lewis X/A (sLeX/A )	220:245	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	1	10	theme	fucosylated	191:201	arg1	glycans					203:209	fucosylated glycans	191:209	fucosylated glycans	191:209	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	4	11	from	adhesion	624:631	arg1	IDC					704:706	IDC	704:706	IDC	704:706	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	11	from	adhesion	624:631	arg1	carcinomas					692:701	invasive ductal carcinomas	676:701	invasive ductal carcinomas (IDC)	676:707	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	5	12	theme	IDC	805:807	arg1	tissue					809:814	IDC tissue	805:814	IDC tissue	805:814	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	4	13	theme	cell	634:637	arg1	signaling					639:647	cell signaling	634:647	cell signaling	634:647	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	11	14	theme	licenses	1899:1906	arg1	adhesion					1956:1963	cell adhesion	1951:1963	cell adhesion	1951:1963	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	14	theme	licenses	1899:1906	arg1	features					1926:1933	fucosylation licenses several malignant features	1886:1933	fucosylation licenses several malignant features	1886:1933	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	14	theme	licenses	1899:1906	arg1	proliferation					1977:1989	proliferation	1977:1989	proliferation	1977:1989	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	14	theme	licenses	1899:1906	arg1	expression					2010:2019	growth factor expression	1996:2019	growth factor expression	1996:2019	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	14	theme	licenses	1899:1906	arg1	migration					1966:1974	migration	1966:1974	migration	1966:1974	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	8	15	theme	CF1_T	1367:1371	arg1	cells					1373:1377	CF1_T cells	1367:1377	CF1_T cells	1367:1377	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	0	16	theme	MAPK	139:142	arg1	activation					144:153	ERK1/2 and p38 MAPK activation	124:153	ERK1/2 and p38 MAPK activation	124:153	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	10	17	theme	factor	1707:1712	arg1	beta					1714:1717	transforming growth factor beta	1687:1717	transforming growth factor beta	1687:1717	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	11	18	theme	growth	1996:2001	arg1	expression					2010:2019	growth factor expression	1996:2019	growth factor expression	1996:2019	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	7	19	with	line	1140:1143	arg1	transcriptase					1175:1187	human telomerase reverse transcriptase	1150:1187	human telomerase reverse transcriptase	1150:1187	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	5	20	theme	ligands	794:800	arg1	expression					769:778	expression	769:778	expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue	769:868	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	8	21	theme	flow	1411:1414	arg1	conditions					1416:1425	hemodynamic flow conditions	1399:1425	hemodynamic flow conditions	1399:1425	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	4	22	theme	ductal	685:690	arg1	IDC					704:706	IDC	704:706	IDC	704:706	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	22	theme	ductal	685:690	arg1	carcinomas					692:701	invasive ductal carcinomas	676:701	invasive ductal carcinomas (IDC)	676:707	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	0	23	theme	ligand	81:86	arg1	expression					88:97	E-selectin ligand expression	70:97	E-selectin ligand expression	70:97	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	5	24	from	tissue	863:868	arg1	tissue					809:814	IDC tissue	805:814	IDC tissue	805:814	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	5	24	from	tissue	863:868	arg1	expression					769:778	expression	769:778	expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue	769:868	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	5	24	from	tissue	863:868	arg1	cultures					845:852	established primary cell cultures	820:852	established primary cell cultures	820:852	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	1	25	theme	Breast	156:161	arg1	tissue					170:175	Breast cancer tissue	156:175	Breast cancer tissue	156:175	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	6	26	theme	cell	967:970	arg1	lines					972:976	cell lines	967:976	cell lines	967:976	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	0	27	from	Inhibition	0:9	arg1	carcinoma					52:60	human invasive ductal carcinoma	30:60	human invasive ductal carcinoma	30:60	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	6	28	dep	FUTs	1005:1008	arg1	FUTs					1005:1008	α-1,3/4 FUTs	997:1008	α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11	997:1043	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	6	28	dep	FUTs	1005:1008	arg1	FUT4					1010:1013	FUT4	1010:1013	FUT4	1010:1013	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	6	28	dep	FUTs	1005:1008	arg1	FUT10					1028:1032	FUT10	1028:1032	FUT10	1028:1032	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	6	28	dep	FUTs	1005:1008	arg1	FUT11					1039:1043	FUT11	1039:1043	FUT11	1039:1043	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	6	28	dep	FUTs	1005:1008	arg1	FUT5					1016:1019	FUT5	1016:1019	FUT5	1016:1019	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	6	28	dep	FUTs	1005:1008	arg1	FUT6					1022:1025	FUT6	1022:1025	FUT6	1022:1025	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	7	29	theme	primary	1123:1129	arg1	line					1140:1143	a primary IDC cell line	1121:1143	a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'	1121:1219	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	4	30	theme	cancer	743:748	arg1	signaling					639:647	cell signaling	634:647	cell signaling	634:647	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	30	theme	cancer	743:748	arg1	proliferation					659:671	cell proliferation	654:671	cell proliferation in invasive ductal carcinomas (IDC)	654:707	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	30	theme	cancer	743:748	arg1	adhesion					624:631	cell adhesion	619:631	cell adhesion	619:631	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	30	theme	cancer	743:748	arg1	type					728:731	the most frequent type	710:731	the most frequent type of breast cancer	710:748	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	5	31	from	expression	769:778	arg1	tissue					863:868	the tissue	859:868	the tissue	859:868	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	5	31	from	expression	769:778	arg1	tissue					809:814	IDC tissue	805:814	IDC tissue	805:814	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	5	31	from	expression	769:778	arg1	cultures					845:852	established primary cell cultures	820:852	established primary cell cultures	820:852	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	9	32	theme	reduced	1475:1481	arg1	ability					1493:1499	a reduced migratory ability	1473:1499	a reduced migratory ability	1473:1499	In addition, 2-FF-treated CF1_T cells showed a reduced migratory ability, as well as decreased cell proliferation rate.					
29215790	8	33	theme	fucosylation	1262:1273	arg1	inhibitor					1275:1283	a fucosylation inhibitor	1260:1283	a fucosylation inhibitor	1260:1283	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	8	33	theme	fucosylation	1262:1273	arg1	2-fluorofucose					1237:1250	2-fluorofucose	1237:1250	2-fluorofucose (2-FF)	1237:1257	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	10	34	theme	2-FF	1557:1560	arg1	treatment					1562:1570	2-FF treatment	1557:1570	2-FF treatment	1557:1570	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	10	35	theme	kinases	1784:1790	arg1	activation					1744:1753	activation	1744:1753	activation of signal-regulating protein kinases 1 and 2	1744:1798	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	10	35	theme	kinases	1784:1790	arg1	pathways					1851:1858	p38 mitogen-activated protein kinase signaling pathways	1804:1858	p38 mitogen-activated protein kinase signaling pathways	1804:1858	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	2	36	theme	tumor	440:444	arg1	extravasation					446:458	tumor extravasation	440:458	tumor extravasation	440:458	These glycans in tumor circulating cells mediate binding to vascular E-selectin, initiating tumor extravasation.					
29215790	4	37	theme	frequent	719:726	arg1	type					728:731	the most frequent type	710:731	the most frequent type of breast cancer	710:748	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	0	38	theme	fucosylation	14:25	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of fucosylation in human invasive ductal carcinoma	0:60	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	11	39	theme	IDC	1938:1940	arg1	adhesion					1956:1963	cell adhesion	1951:1963	cell adhesion	1951:1963	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	39	theme	IDC	1938:1940	arg1	features					1926:1933	fucosylation licenses several malignant features	1886:1933	fucosylation licenses several malignant features	1886:1933	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	39	theme	IDC	1938:1940	arg1	proliferation					1977:1989	proliferation	1977:1989	proliferation	1977:1989	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	39	theme	IDC	1938:1940	arg1	expression					2010:2019	growth factor expression	1996:2019	growth factor expression	1996:2019	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	39	theme	IDC	1938:1940	arg1	migration					1966:1974	migration	1966:1974	migration	1966:1974	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	6	40	theme	FUTs	1005:1008	arg1	expression					983:992	expression	983:992	expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11	983:1043	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	6	40	theme	FUTs	1005:1008	arg1	reactivity					890:899	strong reactivity	883:899	strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines	883:976	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	7	41	gly	fucosylation	1076:1087	arg1	IDC					1092:1094	IDC biology	1092:1102	IDC biology	1092:1102	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	2	42	theme	vascular	408:415	arg1	E-selectin					417:426	vascular E-selectin	408:426	vascular E-selectin	408:426	These glycans in tumor circulating cells mediate binding to vascular E-selectin, initiating tumor extravasation.					
29215790	5	43	theme	established	820:830	arg1	cultures					845:852	established primary cell cultures	820:852	established primary cell cultures	820:852	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	0	44	theme	human	30:34	arg1	carcinoma					52:60	human invasive ductal carcinoma	30:60	human invasive ductal carcinoma	30:60	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	7	45	theme	cell	1210:1213	arg1	line					1215:1218	the 'CF1_T cell line'	1199:1219	the 'CF1_T cell line'	1199:1219	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	10	46	theme	signal-regulating	1758:1774	arg1	kinases					1784:1790	signal-regulating protein kinases 1 and 2	1758:1798	signal-regulating protein kinases 1 and 2	1758:1798	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	8	47	theme	sLeX/A	1311:1316	arg1	expression					1318:1327	its sLeX/A expression	1307:1327	its sLeX/A expression	1307:1327	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	0	48	theme	ductal	45:50	arg1	carcinoma					52:60	human invasive ductal carcinoma	30:60	human invasive ductal carcinoma	30:60	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	1	49	theme	increased	302:310	arg1	progression					320:330	increased disease progression	302:330	increased disease progression	302:330	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	9	50	theme	cell	1523:1526	arg1	rate					1542:1545	decreased cell proliferation rate	1513:1545	decreased cell proliferation rate	1513:1545	In addition, 2-FF-treated CF1_T cells showed a reduced migratory ability, as well as decreased cell proliferation rate.					
29215790	11	51	theme	malignant	1916:1924	arg1	adhesion					1956:1963	cell adhesion	1951:1963	cell adhesion	1951:1963	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	51	theme	malignant	1916:1924	arg1	features					1926:1933	fucosylation licenses several malignant features	1886:1933	fucosylation licenses several malignant features	1886:1933	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	51	theme	malignant	1916:1924	arg1	proliferation					1977:1989	proliferation	1977:1989	proliferation	1977:1989	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	51	theme	malignant	1916:1924	arg1	expression					2010:2019	growth factor expression	1996:2019	growth factor expression	1996:2019	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	51	theme	malignant	1916:1924	arg1	migration					1966:1974	migration	1966:1974	migration	1966:1974	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	3	52	from	role	476:479	arg1	carcinogenesis					491:504	breast carcinogenesis	484:504	breast carcinogenesis	484:504	However, their role in breast carcinogenesis is still unknown.					
29215790	10	53	theme	CF1_T	1612:1616	arg1	cells					1618:1622	CF1_T cells	1612:1622	CF1_T cells	1612:1622	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	1	54	theme	sialyl-Lewis	220:231	arg1	sLeX/A					238:243	sLeX/A	238:243	sLeX/A	238:243	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	1	54	theme	sialyl-Lewis	220:231	arg1	X/A					233:235	sialyl-Lewis X/A	220:235	sialyl-Lewis X/A (sLeX/A )	220:245	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	2	55	theme	tumor	365:369	arg1	cells					383:387	tumor circulating cells	365:387	tumor circulating cells	365:387	These glycans in tumor circulating cells mediate binding to vascular E-selectin, initiating tumor extravasation.					
29215790	7	56	theme	fucosylation	1076:1087	arg1	role					1068:1071	the role	1064:1071	the role of fucosylation in IDC biology	1064:1102	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	10	57	theme	kinase	1834:1839	arg1	pathways					1851:1858	p38 mitogen-activated protein kinase signaling pathways	1804:1858	p38 mitogen-activated protein kinase signaling pathways	1804:1858	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	7	58	from	role	1068:1071	arg1	biology					1096:1102	IDC biology	1092:1102	IDC biology	1092:1102	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	7	59	theme	telomerase	1156:1165	arg1	transcriptase					1175:1187	human telomerase reverse transcriptase	1150:1187	human telomerase reverse transcriptase	1150:1187	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	10	60	theme	mitogen-activated	1808:1824	arg1	kinase					1834:1839	p38 mitogen-activated protein kinase	1804:1839	p38 mitogen-activated protein kinase signaling pathways	1804:1858	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	1	61	gly	fucosylated	191:201	arg1	X/A					233:235	sialyl-Lewis X/A	220:235	sialyl-Lewis X/A (sLeX/A )	220:245	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	1	61	gly	fucosylated	191:201	arg1	glycans					203:209	fucosylated glycans	191:209	fucosylated glycans	191:209	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	4	62	from	proliferation	659:671	arg1	IDC					704:706	IDC	704:706	IDC	704:706	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	62	from	proliferation	659:671	arg1	carcinomas					692:701	invasive ductal carcinomas	676:701	invasive ductal carcinomas (IDC)	676:707	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	63	theme	structures	585:594	arg1	contribution					553:564	the contribution	549:564	the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer	549:748	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	10	64	theme	endothelial	1656:1666	arg1	factor					1675:1680	vascular endothelial growth factor	1647:1680	vascular endothelial growth factor	1647:1680	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	1	65	theme	cancer	163:168	arg1	tissue					170:175	Breast cancer tissue	156:175	Breast cancer tissue	156:175	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	10	66	theme	growth	1584:1589	arg1	factor					1591:1596	the growth factor	1580:1596	the growth factor expression of CF1_T cells	1580:1622	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	2	67	from	glycans	354:360	arg1	cells					383:387	tumor circulating cells	365:387	tumor circulating cells	365:387	These glycans in tumor circulating cells mediate binding to vascular E-selectin, initiating tumor extravasation.					
29215790	7	68	theme	cell	1135:1138	arg1	line					1140:1143	a primary IDC cell line	1121:1143	a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'	1121:1219	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	10	69	theme	growth	1700:1705	arg1	beta					1714:1717	transforming growth factor beta	1687:1717	transforming growth factor beta	1687:1717	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	4	70	from	signaling	639:647	arg1	IDC					704:706	IDC	704:706	IDC	704:706	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	70	from	signaling	639:647	arg1	carcinomas					692:701	invasive ductal carcinomas	676:701	invasive ductal carcinomas (IDC)	676:707	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	5	71	theme	cell	840:843	arg1	cultures					845:852	established primary cell cultures	820:852	established primary cell cultures	820:852	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	6	72	theme	IDC	952:954	arg1	tissue					956:961	IDC tissue	952:961	IDC tissue	952:961	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	0	73	theme	ERK1/2	124:129	arg1	activation					144:153	ERK1/2 and p38 MAPK activation	124:153	ERK1/2 and p38 MAPK activation	124:153	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	11	74	theme	cell	1951:1954	arg1	adhesion					1956:1963	cell adhesion	1951:1963	cell adhesion	1951:1963	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	9	75	theme	CF1_T	1454:1458	arg1	cells					1460:1464	2-FF-treated CF1_T cells	1441:1464	2-FF-treated CF1_T cells	1441:1464	In addition, 2-FF-treated CF1_T cells showed a reduced migratory ability, as well as decreased cell proliferation rate.					
29215790	4	76	theme	cell	619:622	arg1	adhesion					624:631	cell adhesion	619:631	cell adhesion	619:631	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	0	77	theme	p38	135:137	arg1	MAPK					139:142	p38 MAPK	135:142	p38 MAPK	135:142	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	11	78	theme	fucosylation	1886:1897	arg1	adhesion					1956:1963	cell adhesion	1951:1963	cell adhesion	1951:1963	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	78	theme	fucosylation	1886:1897	arg1	features					1926:1933	fucosylation licenses several malignant features	1886:1933	fucosylation licenses several malignant features	1886:1933	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	78	theme	fucosylation	1886:1897	arg1	proliferation					1977:1989	proliferation	1977:1989	proliferation	1977:1989	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	78	theme	fucosylation	1886:1897	arg1	expression					2010:2019	growth factor expression	1996:2019	growth factor expression	1996:2019	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	78	theme	fucosylation	1886:1897	arg1	migration					1966:1974	migration	1966:1974	migration	1966:1974	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	6	79	theme	anti-sLeX/A	921:931	arg1	antibodies					933:942	E-selectin and anti-sLeX/A antibodies	906:942	E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines	906:976	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	8	80	theme	cells	1373:1377	arg1	adherence					1354:1362	adherence	1354:1362	adherence of CF1_T cells to E-selectin	1354:1391	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	6	81	theme	E-selectin	906:915	arg1	antibodies					933:942	E-selectin and anti-sLeX/A antibodies	906:942	E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines	906:976	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	6	82	with	expression	983:992	arg1	antibodies					933:942	E-selectin and anti-sLeX/A antibodies	906:942	E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines	906:976	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	11	83	theme	factor	2003:2008	arg1	expression					2010:2019	growth factor expression	1996:2019	growth factor expression	1996:2019	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	8	84	theme	hemodynamic	1399:1409	arg1	conditions					1416:1425	hemodynamic flow conditions	1399:1425	hemodynamic flow conditions	1399:1425	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	0	85	theme	E-selectin	70:79	arg1	expression					88:97	E-selectin ligand expression	70:97	E-selectin ligand expression	70:97	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	11	86	theme	several	1908:1914	arg1	adhesion					1956:1963	cell adhesion	1951:1963	cell adhesion	1951:1963	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	86	theme	several	1908:1914	arg1	features					1926:1933	fucosylation licenses several malignant features	1886:1933	fucosylation licenses several malignant features	1886:1933	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	86	theme	several	1908:1914	arg1	proliferation					1977:1989	proliferation	1977:1989	proliferation	1977:1989	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	86	theme	several	1908:1914	arg1	expression					2010:2019	growth factor expression	1996:2019	growth factor expression	1996:2019	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	11	86	theme	several	1908:1914	arg1	migration					1966:1974	migration	1966:1974	migration	1966:1974	These data indicate that fucosylation licenses several malignant features of IDC, such as cell adhesion, migration, proliferation, and growth factor expression, contributing to tumor progression.					
29215790	7	87	theme	IDC	1131:1133	arg1	line					1140:1143	a primary IDC cell line	1121:1143	a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'	1121:1219	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	9	88	theme	2-FF-treated	1441:1452	arg1	cells					1460:1464	2-FF-treated CF1_T cells	1441:1464	2-FF-treated CF1_T cells	1441:1464	In addition, 2-FF-treated CF1_T cells showed a reduced migratory ability, as well as decreased cell proliferation rate.					
29215790	4	89	theme	invasive	676:683	arg1	IDC					704:706	IDC	704:706	IDC	704:706	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	89	theme	invasive	676:683	arg1	carcinomas					692:701	invasive ductal carcinomas	676:701	invasive ductal carcinomas (IDC)	676:707	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	0	90	theme	cell	100:103	arg1	proliferation					105:117	cell proliferation	100:117	cell proliferation	100:117	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	4	91	from	type	728:731	arg1	IDC					704:706	IDC	704:706	IDC	704:706	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	91	from	type	728:731	arg1	carcinomas					692:701	invasive ductal carcinomas	676:701	invasive ductal carcinomas (IDC)	676:707	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	92	theme	breast	736:741	arg1	cancer					743:748	breast cancer	736:748	breast cancer	736:748	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	93	gly	fucosylated	573:583	arg1	structures					585:594	the fucosylated structures	569:594	the fucosylated structures	569:594	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	4	93	gly	fucosylated	573:583	arg1	sLeX/A					607:612	sLeX/A	607:612	sLeX/A	607:612	Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling, and cell proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer.					
29215790	9	94	theme	migratory	1483:1491	arg1	ability					1493:1499	a reduced migratory ability	1473:1499	a reduced migratory ability	1473:1499	In addition, 2-FF-treated CF1_T cells showed a reduced migratory ability, as well as decreased cell proliferation rate.					
29215790	2	95	theme	circulating	371:381	arg1	cells					383:387	tumor circulating cells	365:387	tumor circulating cells	365:387	These glycans in tumor circulating cells mediate binding to vascular E-selectin, initiating tumor extravasation.					
29215790	5	96	theme	E-selectin	783:792	arg1	ligands					794:800	E-selectin ligands	783:800	E-selectin ligands	783:800	We first analyzed expression of E-selectin ligands in IDC tissue and established primary cell cultures from the tissue.					
29215790	6	97	from	antibodies	933:942	arg1	tissue					956:961	IDC tissue	952:961	IDC tissue	952:961	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	6	97	from	antibodies	933:942	arg1	lines					972:976	cell lines	967:976	cell lines	967:976	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	10	98	theme	factor	1591:1596	arg1	expression					1598:1607	the growth factor expression	1580:1607	the growth factor expression of CF1_T cells	1580:1622	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	0	99	theme	invasive	36:43	arg1	carcinoma					52:60	human invasive ductal carcinoma	30:60	human invasive ductal carcinoma	30:60	Inhibition of fucosylation in human invasive ductal carcinoma reduces E-selectin ligand expression, cell proliferation, and ERK1/2 and p38 MAPK activation.					
29215790	7	100	theme	CF1_T	1204:1208	arg1	line					1215:1218	the 'CF1_T cell line'	1199:1219	the 'CF1_T cell line'	1199:1219	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	10	101	theme	protein	1776:1782	arg1	kinases					1784:1790	signal-regulating protein kinases 1 and 2	1758:1798	signal-regulating protein kinases 1 and 2	1758:1798	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	9	102	theme	decreased	1513:1521	arg1	rate					1542:1545	decreased cell proliferation rate	1513:1545	decreased cell proliferation rate	1513:1545	In addition, 2-FF-treated CF1_T cells showed a reduced migratory ability, as well as decreased cell proliferation rate.					
29215790	10	103	theme	p38	1804:1806	arg1	kinase					1834:1839	p38 mitogen-activated protein kinase	1804:1839	p38 mitogen-activated protein kinase signaling pathways	1804:1858	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	10	104	theme	cells	1618:1622	arg1	expression					1598:1607	the growth factor expression	1580:1607	the growth factor expression of CF1_T cells	1580:1622	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	1	105	theme	disease	312:318	arg1	progression					320:330	increased disease progression	302:330	increased disease progression	302:330	Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases (FUTs) in relation to increased disease progression and metastasis.					
29215790	9	106	theme	proliferation	1528:1540	arg1	rate					1542:1545	decreased cell proliferation rate	1513:1545	decreased cell proliferation rate	1513:1545	In addition, 2-FF-treated CF1_T cells showed a reduced migratory ability, as well as decreased cell proliferation rate.					
29215790	6	107	with	reactivity	890:899	arg1	antibodies					933:942	E-selectin and anti-sLeX/A antibodies	906:942	E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines	906:976	We observed strong reactivity with E-selectin and anti-sLeX/A antibodies in both IDC tissue and cell lines, and expression of α-1,3/4 FUTs FUT4, FUT5, FUT6, FUT10, and FUT11.					
29215790	8	108	with	Treatment	1222:1230	arg1	inhibitor					1275:1283	a fucosylation inhibitor	1260:1283	a fucosylation inhibitor	1260:1283	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	8	108	with	Treatment	1222:1230	arg1	2-FF					1253:1256	2-FF	1253:1256	2-FF	1253:1256	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	8	108	with	Treatment	1222:1230	arg1	2-fluorofucose					1237:1250	2-fluorofucose	1237:1250	2-fluorofucose (2-FF)	1237:1257	Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions.					
29215790	3	109	theme	breast	484:489	arg1	carcinogenesis					491:504	breast carcinogenesis	484:504	breast carcinogenesis	484:504	However, their role in breast carcinogenesis is still unknown.					
29215790	7	110	theme	reverse	1167:1173	arg1	transcriptase					1175:1187	human telomerase reverse transcriptase	1150:1187	human telomerase reverse transcriptase	1150:1187	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
29215790	10	111	theme	signaling	1841:1849	arg1	pathways					1851:1858	p38 mitogen-activated protein kinase signaling pathways	1804:1858	p38 mitogen-activated protein kinase signaling pathways	1804:1858	Notably, 2-FF treatment lowered the growth factor expression of CF1_T cells, prominently for FGF2, vascular endothelial growth factor, and transforming growth factor beta, and negatively affected activation of signal-regulating protein kinases 1 and 2 and p38 mitogen-activated protein kinase signaling pathways.					
29215790	7	112	theme	IDC	1092:1094	arg1	biology					1096:1102	IDC biology	1092:1102	IDC biology	1092:1102	To further assess the role of fucosylation in IDC biology, we immortalized a primary IDC cell line with human telomerase reverse transcriptase to create the 'CF1_T cell line'.					
31132941	4	0	theme	thermal	798:804	arg1	images					806:811	thermal images	798:811	thermal images	798:811	Finally, the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints were studied with support of the photomicrographs and thermal images.					
31132941	1	1	theme	used	154:157	arg1	materials					183:191	the commonly used thermoplastic composite materials	141:191	the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds	141:246	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	5	2	theme	staring	1037:1043	arg1	time					1045:1048	staring time	1037:1048	staring time as 40 s	1037:1056	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	5	3	with	1000 r/min	983:992	arg1	length					999:1004	length	999:1004	length of consumable tool as 4 mm	999:1031	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	0	4	from	Investigations	0:13	arg1	joining					18:24	joining	18:24	joining	18:24	Investigations on joining of orthopaedic scaffold with rapid tooling.					
31132941	2	5	theme	hydroxyapatite-	298:312	arg1	filament					386:393	hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament	298:393	filament	386:393	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	5	6	theme	tool	1020:1023	arg1	length					999:1004	length	999:1004	length of consumable tool as 4 mm	999:1031	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	3	7	theme	welding	616:622	arg1	process					624:630	friction stir welding process	602:630	friction stir welding process	602:630	In this work, joining of orthopaedic scaffolds has been performed with rapid tooling by using friction stir welding process.					
31132941	4	8	theme	images	806:811	arg1	support					762:768	support	762:768	support of the photomicrographs and thermal images	762:811	Finally, the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints were studied with support of the photomicrographs and thermal images.					
31132941	5	9	theme	orthopaedic	893:903	arg1	scaffolds					905:913	orthopaedic scaffolds	893:913	orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan	893:957	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	4	10	theme	joints	737:742	arg1	properties					657:666	the mechanical properties	642:666	the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints	642:742	Finally, the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints were studied with support of the photomicrographs and thermal images.					
31132941	3	11	theme	orthopaedic	533:543	arg1	scaffolds					545:553	orthopaedic scaffolds	533:553	orthopaedic scaffolds	533:553	In this work, joining of orthopaedic scaffolds has been performed with rapid tooling by using friction stir welding process.					
31132941	5	12	theme	micro	877:881	arg1	cracks					883:888	micro cracks	877:888	micro cracks	877:888	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	4	13	theme	photomicrographs	777:792	arg1	support					762:768	support	762:768	support of the photomicrographs and thermal images	762:811	Finally, the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints were studied with support of the photomicrographs and thermal images.					
31132941	1	14	theme	Poly	70:73	arg1	acid					82:85	Poly lactic acid	70:85	Poly lactic acid reinforced with hydroxyapatite and chitosan	70:129	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	2	15	theme	functional	429:438	arg1	prototypes					440:449	functional prototypes	429:449	functional prototypes	429:449	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	1	16	theme	thermoplastic	159:171	arg1	materials					183:191	the commonly used thermoplastic composite materials	141:191	the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds	141:246	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	5	17	theme	rotational	963:972	arg1	settings					853:860	best settings	848:860	best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan	848:957	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	5	17	theme	rotational	963:972	arg1	speed					974:978	rotational speed	963:978	rotational speed of 1000 r/min with length of consumable tool as 4 mm	963:1031	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	1	18	theme	lactic	75:80	arg1	acid					82:85	Poly lactic acid	70:85	Poly lactic acid reinforced with hydroxyapatite and chitosan	70:129	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	1	19	theme	composite	173:181	arg1	materials					183:191	the commonly used thermoplastic composite materials	141:191	the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds	141:246	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	5	20	theme	best	848:851	arg1	settings					853:860	best settings	848:860	best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan	848:957	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	5	20	theme	best	848:851	arg1	speed					974:978	rotational speed	963:978	rotational speed of 1000 r/min with length of consumable tool as 4 mm	963:1031	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	5	21	from	settings	853:860	arg1	scaffolds					905:913	orthopaedic scaffolds	893:913	orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan	893:957	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	2	22	theme	poly	339:342	arg1	filament					386:393	hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament	298:393	filament	386:393	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	1	23	theme	materials	183:191	arg1	materials					183:191	the commonly used thermoplastic composite materials	141:191	the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds	141:246	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	1	23	theme	materials	183:191	arg1	one					134:136	one	134:136	one	134:136	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	2	24	from	printing	417:424	arg1	deposition					480:489	low cost commercial fused deposition	454:489	low cost commercial fused deposition modelling setup	454:505	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	5	25	theme	acid-hydroxyapatite-chitosan	930:957	arg1	scaffolds					905:913	orthopaedic scaffolds	893:913	orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan	893:957	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	5	26	theme	poly	918:921	arg1	acid-hydroxyapatite-chitosan					930:957	poly lactic acid-hydroxyapatite-chitosan	918:957	poly lactic acid-hydroxyapatite-chitosan	918:957	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	2	27	theme	chitosan-reinforced	318:336	arg1	filament					386:393	hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament	298:393	filament	386:393	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	1	28	theme	three-dimensional	196:212	arg1	printing					214:221	three-dimensional printing	196:221	three-dimensional printing of orthopaedic scaffolds	196:246	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	5	29	theme	1000 r/min	983:992	arg1	settings					853:860	best settings	848:860	best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan	848:957	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	5	29	theme	1000 r/min	983:992	arg1	time					1045:1048	staring time	1037:1048	staring time as 40 s	1037:1056	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	5	29	theme	1000 r/min	983:992	arg1	speed					974:978	rotational speed	963:978	rotational speed of 1000 r/min with length of consumable tool as 4 mm	963:1031	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	3	30	theme	rapid	579:583	arg1	tooling					585:591	rapid tooling	579:591	rapid tooling	579:591	In this work, joining of orthopaedic scaffolds has been performed with rapid tooling by using friction stir welding process.					
31132941	3	31	theme	stir	611:614	arg1	process					624:630	friction stir welding process	602:630	friction stir welding process	602:630	In this work, joining of orthopaedic scaffolds has been performed with rapid tooling by using friction stir welding process.					
31132941	2	32	theme	commercial	463:472	arg1	deposition					480:489	low cost commercial fused deposition	454:489	low cost commercial fused deposition modelling setup	454:505	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	2	33	theme	cost	458:461	arg1	deposition					480:489	low cost commercial fused deposition	454:489	low cost commercial fused deposition modelling setup	454:505	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	0	34	theme	orthopaedic	29:39	arg1	scaffold					41:48	orthopaedic scaffold	29:48	orthopaedic scaffold	29:48	Investigations on joining of orthopaedic scaffold with rapid tooling.					
31132941	5	35	theme	study	829:833	arg1	results					818:824	The results	814:824	The results of study	814:833	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	1	36	theme	orthopaedic	226:236	arg1	scaffolds					238:246	orthopaedic scaffolds	226:246	orthopaedic scaffolds	226:246	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	0	37	theme	rapid	55:59	arg1	tooling					61:67	rapid tooling	55:67	rapid tooling	55:67	Investigations on joining of orthopaedic scaffold with rapid tooling.					
31132941	5	38	theme	lactic	923:928	arg1	acid-hydroxyapatite-chitosan					930:957	poly lactic acid-hydroxyapatite-chitosan	918:957	poly lactic acid-hydroxyapatite-chitosan	918:957	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	2	39	theme	feedstock	376:384	arg1	filament					386:393	hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament	298:393	filament	386:393	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	5	40	theme	consumable	1009:1018	arg1	tool					1020:1023	consumable tool	1009:1023	consumable tool as 4 mm	1009:1031	The results of study suggest that best settings for joining of micro cracks in orthopaedic scaffolds of poly lactic acid-hydroxyapatite-chitosan are rotational speed of 1000 r/min with length of consumable tool as 4 mm and staring time as 40 s.					
31132941	2	41	theme	biocompatible	362:374	arg1	filament					386:393	hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament	298:393	filament	386:393	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	4	42	theme	orthopaedic	715:725	arg1	joints					737:742	the orthopaedic scaffolds joints	711:742	the orthopaedic scaffolds joints	711:742	Finally, the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints were studied with support of the photomicrographs and thermal images.					
31132941	2	43	theme	acid-based	351:360	arg1	filament					386:393	hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament	298:393	filament	386:393	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	3	44	theme	friction	602:609	arg1	process					624:630	friction stir welding process	602:630	friction stir welding process	602:630	In this work, joining of orthopaedic scaffolds has been performed with rapid tooling by using friction stir welding process.					
31132941	1	45	theme	scaffolds	238:246	arg1	printing					214:221	three-dimensional printing	196:221	three-dimensional printing of orthopaedic scaffolds	196:246	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	2	46	theme	work	281:284	arg1	article					254:260	This article	249:260	This article	249:260	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	2	46	theme	work	281:284	arg1	extension					268:276	an extension	265:276	an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup	265:505	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	4	47	dep	properties	657:666	arg1	such					669:672	such	669:672	such	669:672	Finally, the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints were studied with support of the photomicrographs and thermal images.					
31132941	1	48	from	materials	183:191	arg1	printing					214:221	three-dimensional printing	196:221	three-dimensional printing of orthopaedic scaffolds	196:246	Poly lactic acid reinforced with hydroxyapatite and chitosan is one of the commonly used thermoplastic composite materials in three-dimensional printing of orthopaedic scaffolds.					
31132941	2	49	theme	lactic	344:349	arg1	filament					386:393	hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament	298:393	filament	386:393	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	4	50	theme	mechanical	646:655	arg1	properties					657:666	the mechanical properties	642:666	the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints	642:742	Finally, the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints were studied with support of the photomicrographs and thermal images.					
31132941	2	51	theme	low	454:456	arg1	deposition					480:489	low cost commercial fused deposition	454:489	low cost commercial fused deposition modelling setup	454:505	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	2	52	theme	fused	474:478	arg1	deposition					480:489	low cost commercial fused deposition	454:489	low cost commercial fused deposition modelling setup	454:505	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	4	53	theme	scaffolds	727:735	arg1	joints					737:742	the orthopaedic scaffolds joints	711:742	the orthopaedic scaffolds joints	711:742	Finally, the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints were studied with support of the photomicrographs and thermal images.					
31132941	2	54	theme	prototypes	440:449	arg1	printing					417:424	three-dimensional printing	399:424	three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup	399:505	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
31132941	4	55	theme	tensile	677:683	arg1	strength					685:692	tensile strength	677:692	tensile strength	677:692	Finally, the mechanical properties (such as tensile strength and hardness) of the orthopaedic scaffolds joints were studied with support of the photomicrographs and thermal images.					
31132941	2	56	theme	three-dimensional	399:415	arg1	printing					417:424	three-dimensional printing	399:424	three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup	399:505	This article is an extension of work reported on hydroxyapatite- and chitosan-reinforced, poly lactic acid-based biocompatible feedstock filament for three-dimensional printing of functional prototypes on low cost commercial fused deposition modelling setup.					
29593568	6	0	theme	MGAT3	966:970	arg1	silencing					989:997	shRNA silencing	983:997	shRNA silencing	983:997	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	0	theme	MGAT3	966:970	arg1	knockdown					972:980	MGAT3 knockdown	966:980	MGAT3 knockdown (shRNA silencing)	966:998	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	1	1	theme	cancer	274:279	arg1	progression					281:291	cancer progression	274:291	cancer progression	274:291	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	7	2	theme	breast	1267:1272	arg1	cancer					1274:1279	breast cancer	1267:1279	breast cancer	1267:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	7	3	theme	structures	1126:1135	arg1	role					1070:1073	the functional role	1055:1073	the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT	1055:1158	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	2	4	theme	tumor	357:361	arg1	microenvironment					363:378	the tumor microenvironment	353:378	the tumor microenvironment	353:378	EMT can be induced by certain factors, notably hypoxia, in the tumor microenvironment.					
29593568	4	5	dep	strategy	479:486	arg1	spectrometry					494:505	mass spectrometry	489:505	mass spectrometry in combination with lectin microarray analysis	489:552	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	6	theme	EMT	612:614	arg1	model					616:620	a hypoxia-induced EMT model	594:620	a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231	594:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	7	7	theme	dysregulation	1091:1103	arg1	effects					1080:1086	effects	1080:1086	effects of dysregulation	1080:1103	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	4	8	theme	microarray	534:543	arg1	analysis					545:552	lectin microarray analysis	527:552	lectin microarray analysis	527:552	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	5	9	theme	reduced	691:697	arg1	levels					699:704	reduced levels	691:704	reduced levels of bisecting GlcNAc structures	691:735	The model showed reduced levels of bisecting GlcNAc structures, and downregulated expression of the corresponding glycosyltransferase MGAT3.					
29593568	4	10	theme	breast	628:633	arg1	lines					647:651	breast cancer cell lines	628:651	breast cancer cell lines MCF7 and MDA-MB-231	628:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	10	theme	breast	628:633	arg1	MDA-MB-231					662:671	MDA-MB-231	662:671	MDA-MB-231	662:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	10	theme	breast	628:633	arg1	MCF7					653:656	MCF7	653:656	MCF7	653:656	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	3	11	theme	Aberrant	381:388	arg1	levels					390:395	Aberrant levels	381:395	Aberrant levels of certain N-glycans	381:416	Aberrant levels of certain N-glycans is associated with cancer progression.					
29593568	6	12	theme	shRNA	983:987	arg1	silencing					989:997	shRNA silencing	983:997	shRNA silencing	983:997	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	12	theme	shRNA	983:987	arg1	knockdown					972:980	MGAT3 knockdown	966:980	MGAT3 knockdown (shRNA silencing)	966:998	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	7	13	theme	GlcNAc	1119:1124	arg1	structures					1126:1135	bisecting GlcNAc structures	1109:1135	bisecting GlcNAc structures	1109:1135	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	6	14	dep	suppressed	844:853	arg1	whereas					958:964	whereas	958:964	whereas	958:964	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	5	15	theme	corresponding	774:786	arg1	MGAT3					808:812	the corresponding glycosyltransferase MGAT3	770:812	the corresponding glycosyltransferase MGAT3	770:812	The model showed reduced levels of bisecting GlcNAc structures, and downregulated expression of the corresponding glycosyltransferase MGAT3.					
29593568	0	16	from	Transition	88:97	arg1	Cells					116:120	Breast Cancer Cells	102:120	Breast Cancer Cells	102:120	Bisecting N-Acetylglucosamine Structures Inhibit Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells.					
29593568	7	17	theme	hypoxia-induced	1140:1154	arg1	EMT					1156:1158	hypoxia-induced EMT	1140:1158	hypoxia-induced EMT	1140:1158	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	1	18	theme	many	195:198	arg1	processes					211:219	many biological processes	195:219	many biological processes	195:219	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	1	18	theme	many	195:198	arg1	progression					281:291	cancer progression	274:291	cancer progression	274:291	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	1	18	theme	many	195:198	arg1	fibrosis					239:246	tissue fibrosis	232:246	tissue fibrosis	232:246	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	1	18	theme	many	195:198	arg1	diseases					260:267	metastatic diseases	249:267	metastatic diseases	249:267	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	5	19	theme	glycosyltransferase	788:806	arg1	MGAT3					808:812	the corresponding glycosyltransferase MGAT3	770:812	the corresponding glycosyltransferase MGAT3	770:812	The model showed reduced levels of bisecting GlcNAc structures, and downregulated expression of the corresponding glycosyltransferase MGAT3.					
29593568	6	20	theme	AKT	935:937	arg1	pathway					949:955	AKT signaling pathway	935:955	AKT signaling pathway	935:955	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	21	theme	cell	855:858	arg1	migration					860:868	cell migration	855:868	cell migration	855:868	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	0	22	theme	Bisecting	0:8	arg1	Structures					30:39	Bisecting N-Acetylglucosamine Structures	0:39	Bisecting N-Acetylglucosamine Structures	0:39	Bisecting N-Acetylglucosamine Structures Inhibit Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells.					
29593568	7	23	dep	role	1070:1073	arg1	effects					1080:1086	effects	1080:1086	effects of dysregulation	1080:1103	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	1	24	theme	biological	200:209	arg1	processes					211:219	many biological processes	195:219	many biological processes	195:219	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	1	24	theme	biological	200:209	arg1	progression					281:291	cancer progression	274:291	cancer progression	274:291	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	1	24	theme	biological	200:209	arg1	fibrosis					239:246	tissue fibrosis	232:246	tissue fibrosis	232:246	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	1	24	theme	biological	200:209	arg1	diseases					260:267	metastatic diseases	249:267	metastatic diseases	249:267	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	0	25	theme	N-Acetylglucosamine	10:28	arg1	Structures					30:39	Bisecting N-Acetylglucosamine Structures	0:39	Bisecting N-Acetylglucosamine Structures	0:39	Bisecting N-Acetylglucosamine Structures Inhibit Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells.					
29593568	4	26	from	spectrometry	494:505	arg1	combination					510:520	combination	510:520	combination with lectin microarray analysis	510:552	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	5	27	theme	MGAT3	808:812	arg1	expression					756:765	expression	756:765	expression of the corresponding glycosyltransferase MGAT3	756:812	The model showed reduced levels of bisecting GlcNAc structures, and downregulated expression of the corresponding glycosyltransferase MGAT3.					
29593568	4	28	theme	mass	489:492	arg1	spectrometry					494:505	mass spectrometry	489:505	mass spectrometry in combination with lectin microarray analysis	489:552	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	6	29	theme	opposite	1004:1011	arg1	effects					1013:1019	opposite effects	1004:1019	opposite effects	1004:1019	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	3	30	theme	N-glycans	408:416	arg1	levels					390:395	Aberrant levels	381:395	Aberrant levels of certain N-glycans	381:416	Aberrant levels of certain N-glycans is associated with cancer progression.					
29593568	7	31	theme	further	1192:1198	arg1	studies					1209:1215	further detailed studies	1192:1215	further detailed studies of physiological functions of these structures in breast cancer	1192:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	1	32	theme	epithelial-mesenchymal	127:148	arg1	EMT					162:164	EMT	162:164	EMT	162:164	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	1	32	theme	epithelial-mesenchymal	127:148	arg1	transition					150:159	epithelial-mesenchymal transition	127:159	The epithelial-mesenchymal transition (EMT) process	123:173	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	7	33	theme	detailed	1200:1207	arg1	studies					1209:1215	further detailed studies	1192:1215	further detailed studies of physiological functions of these structures in breast cancer	1192:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	7	34	theme	bisecting	1109:1117	arg1	structures					1126:1135	bisecting GlcNAc structures	1109:1135	bisecting GlcNAc structures	1109:1135	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	1	35	theme	transition	150:159	arg1	process					167:173	The epithelial-mesenchymal transition (EMT) process	123:173	The epithelial-mesenchymal transition (EMT) process	123:173	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	4	36	theme	hypoxia-induced	596:610	arg1	model					616:620	a hypoxia-induced EMT model	594:620	a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231	594:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	37	theme	cell	642:645	arg1	lines					647:651	breast cancer cell lines	628:651	breast cancer cell lines MCF7 and MDA-MB-231	628:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	37	theme	cell	642:645	arg1	MDA-MB-231					662:671	MDA-MB-231	662:671	MDA-MB-231	662:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	37	theme	cell	642:645	arg1	MCF7					653:656	MCF7	653:656	MCF7	653:656	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	38	theme	integrated	468:477	arg1	strategy					479:486	an integrated strategy	465:486	an integrated strategy (mass spectrometry in combination with lectin microarray analysis)	465:553	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	2	39	theme	certain	316:322	arg1	factors					324:330	certain factors	316:330	certain factors	316:330	EMT can be induced by certain factors, notably hypoxia, in the tumor microenvironment.					
29593568	2	39	theme	certain	316:322	arg1	hypoxia					341:347	hypoxia	341:347	notably hypoxia	333:347	EMT can be induced by certain factors, notably hypoxia, in the tumor microenvironment.					
29593568	1	40	theme	tissue	232:237	arg1	fibrosis					239:246	tissue fibrosis	232:246	tissue fibrosis	232:246	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	0	41	theme	Epithelial-Mesenchymal	65:86	arg1	Transition					88:97	Hypoxia-Induced Epithelial-Mesenchymal Transition	49:97	Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells	49:120	Bisecting N-Acetylglucosamine Structures Inhibit Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells.					
29593568	7	42	theme	functions	1234:1242	arg1	studies					1209:1215	further detailed studies	1192:1215	further detailed studies of physiological functions of these structures in breast cancer	1192:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	6	43	theme	markers	922:928	arg1	pathway					949:955	AKT signaling pathway	935:955	AKT signaling pathway	935:955	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	43	theme	markers	922:928	arg1	expression					904:913	expression	904:913	expression of EMT markers	904:928	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	43	theme	markers	922:928	arg1	proliferation					871:883	proliferation	871:883	proliferation	871:883	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	43	theme	markers	922:928	arg1	formation					893:901	colony formation	886:901	colony formation	886:901	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	43	theme	markers	922:928	arg1	migration					860:868	cell migration	855:868	cell migration	855:868	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	44	contain	had	1000:1002	arg1	silencing					989:997	shRNA silencing	983:997	shRNA silencing	983:997	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	44	contain	had	1000:1002	arg2	effects					1013:1019	opposite effects	1004:1019	opposite effects	1004:1019	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	6	44	contain	had	1000:1002	arg1	knockdown					972:980	MGAT3 knockdown	966:980	MGAT3 knockdown (shRNA silencing)	966:998	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	7	45	from	role	1070:1073	arg1	EMT					1156:1158	hypoxia-induced EMT	1140:1158	hypoxia-induced EMT	1140:1158	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	0	46	theme	Hypoxia-Induced	49:63	arg1	Transition					88:97	Hypoxia-Induced Epithelial-Mesenchymal Transition	49:97	Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells	49:120	Bisecting N-Acetylglucosamine Structures Inhibit Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells.					
29593568	3	47	theme	cancer	437:442	arg1	progression					444:454	cancer progression	437:454	cancer progression	437:454	Aberrant levels of certain N-glycans is associated with cancer progression.					
29593568	6	48	theme	EMT	918:920	arg1	markers					922:928	EMT markers	918:928	EMT markers	918:928	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	4	49	gly	glycosylation	577:589	arg1	model					616:620	a hypoxia-induced EMT model	594:620	a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231	594:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	7	50	theme	useful	1175:1180	arg1	basis					1182:1186	a useful basis	1173:1186	a useful basis for further detailed studies of physiological functions of these structures in breast cancer	1173:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	7	51	from	structures	1253:1262	arg1	cancer					1274:1279	breast cancer	1267:1279	breast cancer	1267:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	6	52	theme	MGAT3	815:819	arg1	overexpression					821:834	MGAT3 overexpression	815:834	MGAT3 overexpression in MCF7	815:842	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	4	53	theme	aberrant	568:575	arg1	glycosylation					577:589	aberrant glycosylation	568:589	aberrant glycosylation	568:589	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	5	54	theme	bisecting	709:717	arg1	structures					726:735	bisecting GlcNAc structures	709:735	bisecting GlcNAc structures	709:735	The model showed reduced levels of bisecting GlcNAc structures, and downregulated expression of the corresponding glycosyltransferase MGAT3.					
29593568	6	55	theme	signaling	939:947	arg1	pathway					949:955	AKT signaling pathway	935:955	AKT signaling pathway	935:955	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	7	56	theme	structures	1253:1262	arg1	functions					1234:1242	physiological functions	1220:1242	physiological functions of these structures in breast cancer	1220:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	5	57	theme	GlcNAc	719:724	arg1	structures					726:735	bisecting GlcNAc structures	709:735	bisecting GlcNAc structures	709:735	The model showed reduced levels of bisecting GlcNAc structures, and downregulated expression of the corresponding glycosyltransferase MGAT3.					
29593568	4	58	dep	lines	647:651	arg1	lines					647:651	breast cancer cell lines	628:651	breast cancer cell lines MCF7 and MDA-MB-231	628:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	58	dep	lines	647:651	arg1	MDA-MB-231					662:671	MDA-MB-231	662:671	MDA-MB-231	662:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	58	dep	lines	647:651	arg1	MCF7					653:656	MCF7	653:656	MCF7	653:656	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	7	59	theme	functional	1059:1068	arg1	role					1070:1073	the functional role	1055:1073	the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT	1055:1158	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	6	60	from	overexpression	821:834	arg1	MCF7					839:842	MCF7	839:842	MCF7	839:842	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	1	61	theme	metastatic	249:258	arg1	diseases					260:267	metastatic diseases	249:267	metastatic diseases	249:267	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	5	62	theme	structures	726:735	arg1	levels					699:704	reduced levels	691:704	reduced levels of bisecting GlcNAc structures	691:735	The model showed reduced levels of bisecting GlcNAc structures, and downregulated expression of the corresponding glycosyltransferase MGAT3.					
29593568	7	63	from	cancer	1274:1279	arg1	functions					1234:1242	physiological functions	1220:1242	physiological functions of these structures in breast cancer	1220:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	4	64	theme	cancer	635:640	arg1	lines					647:651	breast cancer cell lines	628:651	breast cancer cell lines MCF7 and MDA-MB-231	628:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	64	theme	cancer	635:640	arg1	MDA-MB-231					662:671	MDA-MB-231	662:671	MDA-MB-231	662:671	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	4	64	theme	cancer	635:640	arg1	MCF7					653:656	MCF7	653:656	MCF7	653:656	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	0	65	theme	Cancer	109:114	arg1	Cells					116:120	Breast Cancer Cells	102:120	Breast Cancer Cells	102:120	Bisecting N-Acetylglucosamine Structures Inhibit Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells.					
29593568	6	66	theme	colony	886:891	arg1	formation					893:901	colony formation	886:901	colony formation	886:901	MGAT3 overexpression in MCF7 suppressed cell migration, proliferation, colony formation, expression of EMT markers, and AKT signaling pathway, whereas MGAT3 knockdown (shRNA silencing) had opposite effects.					
29593568	4	67	with	combination	510:520	arg1	analysis					545:552	lectin microarray analysis	527:552	lectin microarray analysis	527:552	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	3	68	theme	certain	400:406	arg1	N-glycans					408:416	certain N-glycans	400:416	certain N-glycans	400:416	Aberrant levels of certain N-glycans is associated with cancer progression.					
29593568	0	69	theme	Breast	102:107	arg1	Cells					116:120	Breast Cancer Cells	102:120	Breast Cancer Cells	102:120	Bisecting N-Acetylglucosamine Structures Inhibit Hypoxia-Induced Epithelial-Mesenchymal Transition in Breast Cancer Cells.					
29593568	4	70	theme	lectin	527:532	arg1	analysis					545:552	lectin microarray analysis	527:552	lectin microarray analysis	527:552	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
29593568	7	71	from	functions	1234:1242	arg1	cancer					1274:1279	breast cancer	1267:1279	breast cancer	1267:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	1	72	theme	key	183:185	arg1	role					187:190	a key role	181:190	a key role	181:190	The epithelial-mesenchymal transition (EMT) process plays a key role in many biological processes, including tissue fibrosis, metastatic diseases, and cancer progression.					
29593568	7	73	theme	physiological	1220:1232	arg1	functions					1234:1242	physiological functions	1220:1242	physiological functions of these structures in breast cancer	1220:1279	Our findings clearly demonstrate the functional role (and effects of dysregulation) of bisecting GlcNAc structures in hypoxia-induced EMT, and provide a useful basis for further detailed studies of physiological functions of these structures in breast cancer.					
29593568	4	74	used	used	460:463	arg2	We					457:458	We	457:458	We	457:458	We used an integrated strategy (mass spectrometry in combination with lectin microarray analysis) to elucidate aberrant glycosylation in a hypoxia-induced EMT model using breast cancer cell lines MCF7 and MDA-MB-231.					
30940835	3	0	gly	desialylated	932:943	arg1	Aβ1-15					945:950	desialylated Aβ1-15 (and Aβ1-17) glycopeptides	932:977	Aβ1-15	945:950	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	5	1	theme	Aβ	1682:1683	arg1	glycopeptides					1685:1697	individual Aβ glycopeptides	1671:1697	individual Aβ glycopeptides	1671:1697	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	2	2	theme	relative	538:545	arg1	increase					547:554	a relative increase	536:554	a relative increase of those in AD vs non-AD patients	536:588	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	3	3	theme	double-labeled	650:663	arg1	glycopeptide					672:683	an isotopically double-labeled Aβ1-15 glycopeptide	634:683	an isotopically double-labeled Aβ1-15 glycopeptide	634:683	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	4	4	theme	oxonium	1289:1295	arg1	fragments					1301:1309	both peptide and oxonium ion fragments	1272:1309	fragments	1301:1309	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	5	5	gly	glycopeptide	1473:1484	arg2	glycopeptide					1473:1484	double-labeled glycopeptide standards	1458:1494	double-labeled glycopeptide standards	1458:1494	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	3	6	dep	establish	885:893	arg1	2					882:882	2	882:882	2	882:882	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	7	theme	glycopeptide	672:683	arg1	use					627:629	use	627:629	use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls	627:1092	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	7	theme	glycopeptide	672:683	arg1	synthesis					613:621	the synthesis	609:621	the synthesis	609:621	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	1	8	theme	amyloid	349:355	arg1	protein					367:373	the amyloid precursor protein	345:373	the amyloid precursor protein (APP)	345:379	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	1	8	theme	amyloid	349:355	arg1	APP					376:378	APP	376:378	APP	376:378	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	2	9	theme	cerebrospinal	497:509	arg1	fluid					511:515	human cerebrospinal fluid	491:515	human cerebrospinal fluid (CSF)	491:521	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	2	9	theme	cerebrospinal	497:509	arg1	CSF					518:520	CSF	518:520	CSF	518:520	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	5	10	theme	glycopeptides	1543:1555	arg1	biomarkers					1569:1578	clinical biomarkers	1560:1578	clinical biomarkers	1560:1578	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	5	10	theme	glycopeptides	1543:1555	arg1	studies					1532:1538	future studies	1525:1538	future studies of glycopeptides	1525:1555	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	0	11	theme	patients	159:166	arg1	fluid					130:134	cerebrospinal fluid	116:134	cerebrospinal fluid of Alzheimer's disease patients	116:166	Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients.					
30940835	2	12	from	patients	581:588	arg1	increase					547:554	a relative increase	536:554	a relative increase of those in AD vs non-AD patients	536:588	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	5	13	theme	clinical	1560:1567	arg1	biomarkers					1569:1578	clinical biomarkers	1560:1578	clinical biomarkers	1560:1578	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	5	13	theme	clinical	1560:1567	arg1	studies					1532:1538	future studies	1525:1538	future studies of glycopeptides	1525:1555	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	3	14	theme	core	699:702	arg1	structure					729:737	the core 1 Galβ3GalNAcα1-O-Tyr-10 structure	695:737	the core 1 Galβ3GalNAcα1-O-Tyr-10 structure	695:737	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	15	dep	identify	747:754	arg1	1					744:744	1	744:744	1	744:744	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	16	theme	glycan	785:790	arg1	structure					799:807	the definite glycan core 1 structure	772:807	the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF	772:872	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	4	17	theme	glycopeptides	1349:1361	arg1	differences					1240:1250	any quantitative differences	1223:1250	any quantitative differences	1223:1250	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	0	18	theme	amyloid-beta	75:86	arg1	glycopeptides					88:100	amyloid-beta glycopeptides	75:100	amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients	75:166	Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients.					
30940835	1	19	theme	amino	303:307	arg1	acids					309:313	up to 43 amino acids	294:313	up to 43 amino acids	294:313	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	1	20	theme	early	172:176	arg1	deposits					246:253	amyloid-β (Aβ) deposits	231:253	amyloid-β (Aβ) deposits	231:253	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	1	20	theme	early	172:176	arg1	hallmark					191:198	An early pathological hallmark	169:198	An early pathological hallmark of Alzheimer's disease (AD)	169:226	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	2	21	theme	peptides	455:462	arg1	series					409:414	a series	407:414	a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF)	407:521	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	3	22	theme	HCD	759:761	arg1	LC-MS/MS					763:770	HCD LC-MS/MS	759:770	HCD LC-MS/MS	759:770	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	1	23	theme	long	315:318	arg1	peptides					323:330	long Aβ peptides	315:330	long Aβ peptides derived from the amyloid precursor protein (APP)	315:379	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	5	24	gly	sialylation	1647:1657	arg1	glycopeptides					1685:1697	individual Aβ glycopeptides	1671:1697	individual Aβ glycopeptides	1671:1697	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	4	25	theme	desialylated	1319:1330	arg1	Aβ1-15					1332:1337	the desialylated Aβ1-15 or Aβ1-17 glycopeptides	1315:1361	Aβ1-15	1332:1337	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	0	26	theme	cerebrospinal	116:128	arg1	fluid					130:134	cerebrospinal fluid	116:134	cerebrospinal fluid of Alzheimer's disease patients	116:166	Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients.					
30940835	0	27	gly	glycopeptides	88:100	arg2	glycopeptides					88:100	amyloid-beta glycopeptides	75:100	amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients	75:166	Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients.					
30940835	3	28	theme	Aβ	844:845	arg1	glycopeptides					847:859	immunopurified and desialylated Aβ glycopeptides	812:859	immunopurified and desialylated Aβ glycopeptides	812:859	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	1	29	dep	43	300:301	arg1	to					297:298	to	297:298	to	297:298	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	5	30	gly	sialylated	1604:1613	arg1	standards					1618:1626	sialylated Aβ standards	1604:1626	sialylated Aβ standards	1604:1626	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	2	31	theme	non-AD	574:579	arg1	patients					581:588	AD vs non-AD patients	568:588	patients	581:588	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	3	32	gly	glycopeptide	672:683	arg2	glycopeptide					672:683	an isotopically double-labeled Aβ1-15 glycopeptide	634:683	an isotopically double-labeled Aβ1-15 glycopeptide	634:683	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	5	33	theme	specific	1638:1645	arg1	patterns					1659:1666	specific sialylation patterns	1638:1666	specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls	1638:1725	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	3	34	dep	use	627:629	arg1	identify					747:754	identify	747:754	to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF	740:872	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	34	dep	use	627:629	arg1	establish					885:893	establish	885:893	to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls	878:1092	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	35	theme	Aβ	1026:1027	arg1	glycopeptides					1029:1041	these Aβ glycopeptides	1020:1041	these Aβ glycopeptides	1020:1041	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	2	36	theme	O-glycosylated	437:450	arg1	peptides					455:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	2	37	from	increase	547:554	arg1	patients					581:588	AD vs non-AD patients	568:588	patients	581:588	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	5	38	theme	Aβ	1615:1616	arg1	standards					1618:1626	sialylated Aβ standards	1604:1626	sialylated Aβ standards	1604:1626	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	2	39	theme	sialylated	419:428	arg1	peptides					455:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	5	40	theme	new	1400:1402	arg1	approach					1432:1439	The new quantitative glycoproteomic approach	1396:1439	The new quantitative glycoproteomic approach described	1396:1449	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	4	41	theme	glycopeptides	1189:1201	arg1	sites					1176:1180	Tyr-10 attachment sites	1158:1180	Tyr-10 attachment sites	1158:1180	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	4	41	theme	glycopeptides	1189:1201	arg1	glycopeptides					1189:1201	the glycopeptides	1185:1201	the glycopeptides	1185:1201	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	4	41	theme	glycopeptides	1189:1201	arg1	structures					1143:1152	core 1 structures	1136:1152	core 1 structures	1136:1152	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	5	42	theme	glycopeptides	1685:1697	arg1	patterns					1659:1666	specific sialylation patterns	1638:1666	specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls	1638:1725	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	2	43	gly	sialylated	419:428	arg1	peptides					455:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	5	44	theme	glycoproteomic	1417:1430	arg1	approach					1432:1439	The new quantitative glycoproteomic approach	1396:1439	The new quantitative glycoproteomic approach described	1396:1449	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	5	45	theme	AD	1702:1703	arg1	patients					1705:1712	AD patients	1702:1712	AD patients	1702:1712	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	1	46	theme	amyloid-β	231:239	arg1	deposits					246:253	amyloid-β (Aβ) deposits	231:253	amyloid-β (Aβ) deposits	231:253	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	1	46	theme	amyloid-β	231:239	arg1	hallmark					191:198	An early pathological hallmark	169:198	An early pathological hallmark of Alzheimer's disease (AD)	169:226	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	3	47	theme	LC-MS/MS	897:904	arg1	method					921:926	a LC-MS/MS quantification method	895:926	a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls	895:1092	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	4	48	theme	attachment	1165:1174	arg1	sites					1176:1180	Tyr-10 attachment sites	1158:1180	Tyr-10 attachment sites	1158:1180	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	4	48	theme	attachment	1165:1174	arg1	glycopeptides					1189:1201	the glycopeptides	1185:1201	the glycopeptides	1185:1201	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	1	49	attach	derived	332:338	arg1	protein					367:373	the amyloid precursor protein	345:373	the amyloid precursor protein (APP)	345:379	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	1	49	attach	derived	332:338	arg2	peptides					323:330	long Aβ peptides	315:330	long Aβ peptides derived from the amyloid precursor protein (APP)	315:379	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	1	49	attach	derived	332:338	arg1	APP					376:378	APP	376:378	APP	376:378	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	2	50	from	fluid	511:515	arg1	series					409:414	a series	407:414	a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF)	407:521	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	2	50	from	fluid	511:515	arg1	peptides					455:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	5	51	theme	future	1525:1530	arg1	biomarkers					1569:1578	clinical biomarkers	1560:1578	clinical biomarkers	1560:1578	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	5	51	theme	future	1525:1530	arg1	studies					1532:1538	future studies	1525:1538	future studies of glycopeptides	1525:1555	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	5	52	theme	individual	1671:1680	arg1	glycopeptides					1685:1697	individual Aβ glycopeptides	1671:1697	individual Aβ glycopeptides	1671:1697	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	3	53	theme	AD	1058:1059	arg1	patients					1061:1068	20 AD patients	1055:1068	20 AD patients	1055:1068	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	54	theme	desialylated	932:943	arg1	Aβ1-15					945:950	desialylated Aβ1-15 (and Aβ1-17) glycopeptides	932:977	Aβ1-15	945:950	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	5	55	theme	glycopeptide	1473:1484	arg1	standards					1486:1494	double-labeled glycopeptide standards	1458:1494	double-labeled glycopeptide standards	1458:1494	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	5	56	from	patterns	1659:1666	arg1	controls					1718:1725	controls	1718:1725	controls	1718:1725	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	5	56	from	patterns	1659:1666	arg1	patients					1705:1712	AD patients	1702:1712	AD patients	1702:1712	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	3	57	from	structure	799:807	arg1	CSF					870:872	human CSF	864:872	human CSF	864:872	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	58	theme	Aβ1-15	665:670	arg1	glycopeptide					672:683	an isotopically double-labeled Aβ1-15 glycopeptide	634:683	an isotopically double-labeled Aβ1-15 glycopeptide	634:683	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	4	59	gly	glycopeptides	1349:1361	arg2	glycopeptides					1349:1361	the desialylated Aβ1-15 or Aβ1-17 glycopeptides	1315:1361	glycopeptides	1349:1361	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	3	60	theme	healthy	1077:1083	arg1	controls					1085:1092	20 healthy controls	1074:1092	20 healthy controls	1074:1092	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	2	61	theme	AD	568:569	arg1	patients					581:588	AD vs non-AD patients	568:588	patients	581:588	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	3	62	theme	Aβ1-17	957:962	arg1	glycopeptides					965:977	desialylated Aβ1-15 (and Aβ1-17) glycopeptides	932:977	glycopeptides	965:977	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	63	gly	glycopeptides	1029:1041	arg2	glycopeptides					1029:1041	these Aβ glycopeptides	1020:1041	these Aβ glycopeptides	1020:1041	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	2	64	theme	those	559:563	arg1	increase					547:554	a relative increase	536:554	a relative increase of those in AD vs non-AD patients	536:588	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	0	65	theme	disease	151:157	arg1	patients					159:166	Alzheimer's disease patients	139:166	Alzheimer's disease patients	139:166	Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients.					
30940835	4	66	gly	desialylated	1319:1330	arg1	Aβ1-15					1332:1337	the desialylated Aβ1-15 or Aβ1-17 glycopeptides	1315:1361	Aβ1-15	1332:1337	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	4	67	theme	ion	1297:1299	arg1	fragments					1301:1309	both peptide and oxonium ion fragments	1272:1309	fragments	1301:1309	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	1	68	from	deposits	246:253	arg1	brain					262:266	the brain	258:266	the brain	258:266	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	1	69	theme	precursor	357:365	arg1	protein					367:373	the amyloid precursor protein	345:373	the amyloid precursor protein (APP)	345:379	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	1	69	theme	precursor	357:365	arg1	APP					376:378	APP	376:378	APP	376:378	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	2	70	theme	human	491:495	arg1	fluid					511:515	human cerebrospinal fluid	491:515	human cerebrospinal fluid (CSF)	491:521	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	2	70	theme	human	491:495	arg1	CSF					518:520	CSF	518:520	CSF	518:520	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	3	71	theme	1 Galβ3GalNAcα1-O-Tyr-10	704:727	arg1	structure					729:737	the core 1 Galβ3GalNAcα1-O-Tyr-10 structure	695:737	the core 1 Galβ3GalNAcα1-O-Tyr-10 structure	695:737	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	5	72	gly	glycopeptides	1543:1555	arg2	glycopeptides					1543:1555	glycopeptides	1543:1555	glycopeptides	1543:1555	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	3	73	theme	core	792:795	arg1	structure					799:807	the definite glycan core 1 structure	772:807	the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF	772:872	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	74	gly	glycopeptides	847:859	arg2	glycopeptides					847:859	immunopurified and desialylated Aβ glycopeptides	812:859	immunopurified and desialylated Aβ glycopeptides	812:859	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	4	75	gly	glycopeptides	1189:1201	arg2	glycopeptides					1189:1201	the glycopeptides	1185:1201	the glycopeptides	1185:1201	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	4	76	theme	Aβ1-15	1332:1337	arg1	differences					1240:1250	any quantitative differences	1223:1250	any quantitative differences	1223:1250	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	0	77	theme	glycopeptides	88:100	arg1	quantification					25:38	quantification	25:38	quantification	25:38	Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients.					
30940835	0	77	theme	glycopeptides	88:100	arg1	characterization					55:70	structural characterization	44:70	structural characterization	44:70	Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients.					
30940835	3	78	theme	definite	776:783	arg1	structure					799:807	the definite glycan core 1 structure	772:807	the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF	772:872	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	79	gly	desialylated	831:842	arg1	glycopeptides					847:859	immunopurified and desialylated Aβ glycopeptides	812:859	immunopurified and desialylated Aβ glycopeptides	812:859	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	4	80	theme	Aβ1-17	1342:1347	arg1	glycopeptides					1349:1361	the desialylated Aβ1-15 or Aβ1-17 glycopeptides	1315:1361	glycopeptides	1349:1361	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	1	81	theme	pathological	178:189	arg1	deposits					246:253	amyloid-β (Aβ) deposits	231:253	amyloid-β (Aβ) deposits	231:253	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	1	81	theme	pathological	178:189	arg1	hallmark					191:198	An early pathological hallmark	169:198	An early pathological hallmark of Alzheimer's disease (AD)	169:226	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	3	82	theme	immunopurified	812:825	arg1	glycopeptides					847:859	immunopurified and desialylated Aβ glycopeptides	812:859	immunopurified and desialylated Aβ glycopeptides	812:859	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	83	from	concentrations	1002:1015	arg1	CSF					1046:1048	CSF	1046:1048	CSF	1046:1048	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	2	84	theme	Aβ	452:453	arg1	peptides					455:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	2	85	from	series	409:414	arg1	fluid					511:515	human cerebrospinal fluid	491:515	human cerebrospinal fluid (CSF)	491:521	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	2	85	from	series	409:414	arg1	CSF					518:520	CSF	518:520	CSF	518:520	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	4	86	theme	core	1136:1139	arg1	structures					1143:1152	core 1 structures	1136:1152	core 1 structures	1136:1152	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	1	87	theme	Aβ	320:321	arg1	peptides					323:330	long Aβ peptides	315:330	long Aβ peptides derived from the amyloid precursor protein (APP)	315:379	An early pathological hallmark of Alzheimer's disease (AD) is amyloid-β (Aβ) deposits in the brain, which largely consist of up to 43 amino acids long Aβ peptides derived from the amyloid precursor protein (APP).					
30940835	3	88	theme	glycopeptides	847:859	arg1	structure					799:807	the definite glycan core 1 structure	772:807	the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF	772:872	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	4	89	theme	non-AD	1382:1387	arg1	group					1389:1393	the AD and non-AD group	1371:1393	group	1389:1393	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	3	90	theme	desialylated	831:842	arg1	glycopeptides					847:859	immunopurified and desialylated Aβ glycopeptides	812:859	immunopurified and desialylated Aβ glycopeptides	812:859	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	5	91	theme	sialylation	1647:1657	arg1	patterns					1659:1666	specific sialylation patterns	1638:1666	specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls	1638:1725	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	5	92	gly	glycopeptides	1685:1697	arg2	glycopeptides					1685:1697	individual Aβ glycopeptides	1671:1697	individual Aβ glycopeptides	1671:1697	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	3	93	contain	carrying	686:693	arg1	glycopeptide					672:683	an isotopically double-labeled Aβ1-15 glycopeptide	634:683	an isotopically double-labeled Aβ1-15 glycopeptide	634:683	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	93	contain	carrying	686:693	arg2	structure					729:737	the core 1 Galβ3GalNAcα1-O-Tyr-10 structure	695:737	the core 1 Galβ3GalNAcα1-O-Tyr-10 structure	695:737	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	5	94	theme	sialylated	1604:1613	arg1	standards					1618:1626	sialylated Aβ standards	1604:1626	sialylated Aβ standards	1604:1626	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	0	95	theme	structural	44:53	arg1	characterization					55:70	structural characterization	44:70	structural characterization	44:70	Synthetic standard aided quantification and structural characterization of amyloid-beta glycopeptides enriched from cerebrospinal fluid of Alzheimer's disease patients.					
30940835	3	96	theme	human	864:868	arg1	CSF					870:872	human CSF	864:872	human CSF	864:872	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	2	97	theme	Tyr-10	430:435	arg1	peptides					455:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	sialylated Tyr-10 O-glycosylated Aβ peptides	419:462	We previously identified a series of sialylated Tyr-10 O-glycosylated Aβ peptides, 15-20 residues long, from human cerebrospinal fluid (CSF) and observed a relative increase of those in AD vs non-AD patients.					
30940835	5	98	theme	double-labeled	1458:1471	arg1	standards					1486:1494	double-labeled glycopeptide standards	1458:1494	double-labeled glycopeptide standards	1458:1494	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	4	99	theme	AD	1375:1376	arg1	group					1389:1393	the AD and non-AD group	1371:1393	group	1389:1393	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	3	100	dep	compare	990:996	arg1	3					987:987	3	987:987	3	987:987	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	101	gly	glycopeptides	965:977	arg2	glycopeptides					965:977	desialylated Aβ1-15 (and Aβ1-17) glycopeptides	932:977	glycopeptides	965:977	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	5	102	theme	quantitative	1404:1415	arg1	approach					1432:1439	The new quantitative glycoproteomic approach	1396:1439	The new quantitative glycoproteomic approach described	1396:1449	The new quantitative glycoproteomic approach described, using double-labeled glycopeptide standards, will undoubtedly facilitate future studies of glycopeptides as clinical biomarkers but should also embrace sialylated Aβ standards to reveal specific sialylation patterns of individual Aβ glycopeptides in AD patients and controls.					
30940835	4	103	theme	Tyr-10	1158:1163	arg1	sites					1176:1180	Tyr-10 attachment sites	1158:1180	Tyr-10 attachment sites	1158:1180	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	4	103	theme	Tyr-10	1158:1163	arg1	glycopeptides					1189:1201	the glycopeptides	1185:1201	the glycopeptides	1185:1201	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	3	104	theme	glycopeptides	1029:1041	arg1	concentrations					1002:1015	the concentrations	998:1015	the concentrations of these Aβ glycopeptides in CSF	998:1048	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	3	105	theme	quantification	906:919	arg1	method					921:926	a LC-MS/MS quantification method	895:926	a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls	895:1092	We report here on the synthesis and use of an isotopically double-labeled Aβ1-15 glycopeptide, carrying the core 1 Galβ3GalNAcα1-O-Tyr-10 structure, to (1) identify by HCD LC-MS/MS the definite glycan core 1 structure of immunopurified and desialylated Aβ glycopeptides in human CSF and to (2) establish a LC-MS/MS quantification method for desialylated Aβ1-15 (and Aβ1-17) glycopeptides and to (3) compare the concentrations of these Aβ glycopeptides in CSF from 20 AD patients and 20 healthy controls.					
30940835	4	106	theme	quantitative	1227:1238	arg1	differences					1240:1250	any quantitative differences	1223:1250	any quantitative differences	1223:1250	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
30940835	4	107	dep	structures	1143:1152	arg1	the					1132:1134	the	1132:1134	the	1132:1134	Although we unambiguously identified the core 1 structures and Tyr-10 attachment sites of the glycopeptides, we did not observe any quantitative differences, determined through both peptide and oxonium ion fragments, of the desialylated Aβ1-15 or Aβ1-17 glycopeptides between the AD and non-AD group.					
31227153	0	0	theme	hydrogel	84:91	arg1	scaffold					93:100	3D-printed silk based hydrogel scaffold	62:100	3D-printed silk based hydrogel scaffold	62:100	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	2	1	with	scaffolds	394:402	arg1	properties					427:436	proper mechanical properties	409:436	proper mechanical properties matching the natural tissue	409:464	However, it has been practically difficult to achieve a highly biomimetic hydrogel scaffolds with proper mechanical properties matching the natural tissue.					
31227153	5	2	theme	printed	815:821	arg1	sample					823:828	printed sample	815:828	printed sample	815:828	Results showed that the tensile strength of printed sample increased significantly with the addition of BCNFs in the bioink.					
31227153	0	3	with	stress	39:44	arg1	pores					120:124	hierarchical pores	107:124	hierarchical pores	107:124	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	5	4	theme	tensile	795:801	arg1	strength					803:810	the tensile strength	791:810	the tensile strength of printed sample	791:828	Results showed that the tensile strength of printed sample increased significantly with the addition of BCNFs in the bioink.					
31227153	0	5	theme	based	78:82	arg1	scaffold					93:100	3D-printed silk based hydrogel scaffold	62:100	3D-printed silk based hydrogel scaffold	62:100	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	1	6	theme	tissue	265:270	arg1	regeneration					272:283	tissue regeneration	265:283	tissue regeneration	265:283	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	4	7	theme	hierarchical	692:703	arg1	pores					705:709	hierarchical pores	692:709	hierarchical pores	692:709	The SF-based hydrogel scaffolds with hierarchical pores were fabricated via 3D-printing followed by lyophilization.					
31227153	0	8	theme	scaffold	93:100	arg1	fidelity					50:57	fidelity	50:57	fidelity	50:57	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	0	8	theme	scaffold	93:100	arg1	stress					39:44	stress	39:44	stress	39:44	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	5	9	from	BCNFs	875:879	arg1	bioink					888:893	the bioink	884:893	the bioink	884:893	Results showed that the tensile strength of printed sample increased significantly with the addition of BCNFs in the bioink.					
31227153	3	10	theme	mechanical	574:583	arg1	properties					585:594	mechanical properties	574:594	mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds	574:652	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	8	11	theme	tissue	1376:1381	arg1	applications					1395:1406	further tissue engineering applications	1368:1406	further tissue engineering applications	1368:1406	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	5	12	theme	sample	823:828	arg1	strength					803:810	the tensile strength	791:810	the tensile strength of printed sample	791:828	Results showed that the tensile strength of printed sample increased significantly with the addition of BCNFs in the bioink.					
31227153	7	13	dep	600 μm	1170:1175	arg1	to					1167:1168	to	1167:1168	to	1167:1168	The pores ranging from 10 to 20 μm inside the printed filaments served as host for cellular infiltration, while the pores with a diameter from 300 to 600 μm circled by printed filaments ensured sufficient nutrient supply.					
31227153	1	14	from	trends	145:150	arg1	medicine					172:179	the regenerative medicine	155:179	the regenerative medicine	155:179	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	1	15	theme	regenerative	159:170	arg1	medicine					172:179	the regenerative medicine	155:179	the regenerative medicine	155:179	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	0	16	theme	hierarchical	107:118	arg1	pores					120:124	hierarchical pores	107:124	hierarchical pores	107:124	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	5	17	from	addition	863:870	arg1	bioink					888:893	the bioink	884:893	the bioink	884:893	Results showed that the tensile strength of printed sample increased significantly with the addition of BCNFs in the bioink.					
31227153	4	18	theme	SF-based	659:666	arg1	scaffolds					677:685	The SF-based hydrogel scaffolds	655:685	The SF-based hydrogel scaffolds with hierarchical pores	655:709	The SF-based hydrogel scaffolds with hierarchical pores were fabricated via 3D-printing followed by lyophilization.					
31227153	6	19	from	pores	902:906	arg1	scaffolds					930:938	the scaffolds	926:938	the scaffolds	926:938	Large pores and micropores in the scaffolds were achieved by designing printing pattern and lyophilization after extrusion.					
31227153	2	20	theme	proper	409:414	arg1	properties					427:436	proper mechanical properties	409:436	proper mechanical properties matching the natural tissue	409:464	However, it has been practically difficult to achieve a highly biomimetic hydrogel scaffolds with proper mechanical properties matching the natural tissue.					
31227153	0	21	theme	cellulose	10:18	arg1	nanofibers					20:29	Bacterial cellulose nanofibers	0:29	Bacterial cellulose nanofibers	0:29	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	3	22	theme	structural	540:549	arg1	resolution					551:560	the structural resolution	536:560	the structural resolution	536:560	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	3	23	theme	silk	599:602	arg1	SF					613:614	SF	613:614	SF	613:614	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	3	23	theme	silk	599:602	arg1	fibroin					604:610	silk fibroin	599:610	silk fibroin (SF)/gelatin composite hydrogel scaffolds	599:652	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	7	24	theme	sufficient	1214:1223	arg1	supply					1234:1239	sufficient nutrient supply	1214:1239	sufficient nutrient supply	1214:1239	The pores ranging from 10 to 20 μm inside the printed filaments served as host for cellular infiltration, while the pores with a diameter from 300 to 600 μm circled by printed filaments ensured sufficient nutrient supply.					
31227153	8	25	theme	hierarchical	1321:1332	arg1	structures					1339:1348	hierarchical pore structures	1321:1348	hierarchical pore structures	1321:1348	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	0	26	theme	Bacterial	0:8	arg1	nanofibers					20:29	Bacterial cellulose nanofibers	0:29	Bacterial cellulose nanofibers	0:29	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	1	27	theme	organ	289:293	arg1	reconstruction					295:308	organ reconstruction	289:308	organ reconstruction	289:308	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	3	28	theme	fibroin	604:610	arg1	scaffolds					644:652	silk fibroin (SF)/gelatin composite hydrogel scaffolds	599:652	silk fibroin (SF)/gelatin composite hydrogel scaffolds	599:652	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	8	29	theme	pore	1334:1337	arg1	structures					1339:1348	hierarchical pore structures	1321:1348	hierarchical pore structures	1321:1348	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	5	30	theme	BCNFs	875:879	arg1	addition					863:870	the addition	859:870	the addition of BCNFs in the bioink	859:893	Results showed that the tensile strength of printed sample increased significantly with the addition of BCNFs in the bioink.					
31227153	8	31	theme	3D-printed	1248:1257	arg1	scaffolds					1269:1277	These 3D-printed composite scaffolds	1242:1277	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures	1242:1348	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	2	32	theme	natural	451:457	arg1	tissue					459:464	the natural tissue	447:464	the natural tissue	447:464	However, it has been practically difficult to achieve a highly biomimetic hydrogel scaffolds with proper mechanical properties matching the natural tissue.					
31227153	6	33	from	micropores	912:921	arg1	scaffolds					930:938	the scaffolds	926:938	the scaffolds	926:938	Large pores and micropores in the scaffolds were achieved by designing printing pattern and lyophilization after extrusion.					
31227153	6	34	theme	Large	896:900	arg1	pores					902:906	Large pores	896:906	Large pores	896:906	Large pores and micropores in the scaffolds were achieved by designing printing pattern and lyophilization after extrusion.					
31227153	6	35	theme	printing	967:974	arg1	pattern					976:982	printing pattern	967:982	printing pattern	967:982	Large pores and micropores in the scaffolds were achieved by designing printing pattern and lyophilization after extrusion.					
31227153	1	36	theme	latest	138:143	arg1	trends					145:150	the latest trends	134:150	the latest trends in the regenerative medicine	134:179	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	7	37	theme	cellular	1103:1110	arg1	infiltration					1112:1123	cellular infiltration	1103:1123	cellular infiltration	1103:1123	The pores ranging from 10 to 20 μm inside the printed filaments served as host for cellular infiltration, while the pores with a diameter from 300 to 600 μm circled by printed filaments ensured sufficient nutrient supply.					
31227153	3	38	theme	bacterial	473:481	arg1	BCNFs					505:509	BCNFs	505:509	BCNFs	505:509	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	3	38	theme	bacterial	473:481	arg1	nanofibers					493:502	bacterial cellulose nanofibers	473:502	bacterial cellulose nanofibers (BCNFs)	473:510	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	4	39	theme	hydrogel	668:675	arg1	scaffolds					677:685	The SF-based hydrogel scaffolds	655:685	The SF-based hydrogel scaffolds with hierarchical pores	655:709	The SF-based hydrogel scaffolds with hierarchical pores were fabricated via 3D-printing followed by lyophilization.					
31227153	7	40	dep	20 μm	1049:1053	arg1	to					1046:1047	to	1046:1047	to	1046:1047	The pores ranging from 10 to 20 μm inside the printed filaments served as host for cellular infiltration, while the pores with a diameter from 300 to 600 μm circled by printed filaments ensured sufficient nutrient supply.					
31227153	1	41	theme	3D-printing	203:213	arg1	scaffolds					224:232	3D-printing hydrogel scaffolds	203:232	3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction	203:308	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	2	42	theme	mechanical	416:425	arg1	properties					427:436	proper mechanical properties	409:436	proper mechanical properties matching the natural tissue	409:464	However, it has been practically difficult to achieve a highly biomimetic hydrogel scaffolds with proper mechanical properties matching the natural tissue.					
31227153	7	43	theme	printed	1188:1194	arg1	filaments					1196:1204	printed filaments	1188:1204	printed filaments	1188:1204	The pores ranging from 10 to 20 μm inside the printed filaments served as host for cellular infiltration, while the pores with a diameter from 300 to 600 μm circled by printed filaments ensured sufficient nutrient supply.					
31227153	3	44	theme	/gelatin	616:623	arg1	scaffolds					644:652	silk fibroin (SF)/gelatin composite hydrogel scaffolds	599:652	silk fibroin (SF)/gelatin composite hydrogel scaffolds	599:652	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	7	45	theme	printed	1066:1072	arg1	filaments					1074:1082	the printed filaments	1062:1082	the printed filaments	1062:1082	The pores ranging from 10 to 20 μm inside the printed filaments served as host for cellular infiltration, while the pores with a diameter from 300 to 600 μm circled by printed filaments ensured sufficient nutrient supply.					
31227153	1	46	theme	hydrogel	215:222	arg1	scaffolds					224:232	3D-printing hydrogel scaffolds	203:232	3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction	203:308	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	8	47	theme	remarkable	1284:1293	arg1	properties					1306:1315	remarkable mechanical properties	1284:1315	remarkable mechanical properties	1284:1315	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	8	48	theme	composite	1259:1267	arg1	scaffolds					1269:1277	These 3D-printed composite scaffolds	1242:1277	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures	1242:1348	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	8	49	theme	further	1368:1374	arg1	applications					1395:1406	further tissue engineering applications	1368:1406	further tissue engineering applications	1368:1406	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	4	50	with	scaffolds	677:685	arg1	pores					705:709	hierarchical pores	692:709	hierarchical pores	692:709	The SF-based hydrogel scaffolds with hierarchical pores were fabricated via 3D-printing followed by lyophilization.					
31227153	7	51	with	pores	1136:1140	arg1	diameter					1149:1156	a diameter	1147:1156	a diameter from 300 to 600 μm circled by printed filaments	1147:1204	The pores ranging from 10 to 20 μm inside the printed filaments served as host for cellular infiltration, while the pores with a diameter from 300 to 600 μm circled by printed filaments ensured sufficient nutrient supply.					
31227153	3	52	theme	scaffolds	644:652	arg1	properties					585:594	mechanical properties	574:594	mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds	574:652	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	5	53	from	bioink	888:893	arg1	addition					863:870	the addition	859:870	the addition of BCNFs in the bioink	859:893	Results showed that the tensile strength of printed sample increased significantly with the addition of BCNFs in the bioink.					
31227153	8	54	theme	engineering	1383:1393	arg1	applications					1395:1406	further tissue engineering applications	1368:1406	further tissue engineering applications	1368:1406	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	8	55	with	scaffolds	1269:1277	arg1	structures					1339:1348	hierarchical pore structures	1321:1348	hierarchical pore structures	1321:1348	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	8	55	with	scaffolds	1269:1277	arg1	properties					1306:1315	remarkable mechanical properties	1284:1315	remarkable mechanical properties	1284:1315	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	1	56	theme	scaffolds	224:232	arg1	trends					145:150	the latest trends	134:150	the latest trends in the regenerative medicine	134:179	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	1	56	theme	scaffolds	224:232	arg1	One					127:129	One	127:129	One	127:129	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	1	56	theme	scaffolds	224:232	arg1	development					188:198	the development	184:198	the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction	184:308	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	2	57	theme	biomimetic	374:383	arg1	scaffolds					394:402	a highly biomimetic hydrogel scaffolds	365:402	a highly biomimetic hydrogel scaffolds with proper mechanical properties matching the natural tissue	365:464	However, it has been practically difficult to achieve a highly biomimetic hydrogel scaffolds with proper mechanical properties matching the natural tissue.					
31227153	0	58	theme	silk	73:76	arg1	scaffold					93:100	3D-printed silk based hydrogel scaffold	62:100	3D-printed silk based hydrogel scaffold	62:100	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	3	59	theme	cellulose	483:491	arg1	BCNFs					505:509	BCNFs	505:509	BCNFs	505:509	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	3	59	theme	cellulose	483:491	arg1	nanofibers					493:502	bacterial cellulose nanofibers	473:502	bacterial cellulose nanofibers (BCNFs)	473:510	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	3	60	theme	composite	625:633	arg1	scaffolds					644:652	silk fibroin (SF)/gelatin composite hydrogel scaffolds	599:652	silk fibroin (SF)/gelatin composite hydrogel scaffolds	599:652	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	0	61	with	fidelity	50:57	arg1	pores					120:124	hierarchical pores	107:124	hierarchical pores	107:124	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	2	62	theme	hydrogel	385:392	arg1	scaffolds					394:402	a highly biomimetic hydrogel scaffolds	365:402	a highly biomimetic hydrogel scaffolds with proper mechanical properties matching the natural tissue	365:464	However, it has been practically difficult to achieve a highly biomimetic hydrogel scaffolds with proper mechanical properties matching the natural tissue.					
31227153	8	63	theme	mechanical	1295:1304	arg1	properties					1306:1315	remarkable mechanical properties	1284:1315	remarkable mechanical properties	1284:1315	These 3D-printed composite scaffolds with remarkable mechanical properties and hierarchical pore structures are promising for further tissue engineering applications.					
31227153	1	64	theme	biomimetic	239:248	arg1	structures					250:259	biomimetic structures	239:259	biomimetic structures for tissue regeneration and organ reconstruction	239:308	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	0	65	theme	3D-printed	62:71	arg1	scaffold					93:100	3D-printed silk based hydrogel scaffold	62:100	3D-printed silk based hydrogel scaffold	62:100	Bacterial cellulose nanofibers promote stress and fidelity of 3D-printed silk based hydrogel scaffold with hierarchical pores.					
31227153	7	66	from	600 μm	1170:1175	arg1	diameter					1149:1156	a diameter	1147:1156	a diameter from 300 to 600 μm circled by printed filaments	1147:1204	The pores ranging from 10 to 20 μm inside the printed filaments served as host for cellular infiltration, while the pores with a diameter from 300 to 600 μm circled by printed filaments ensured sufficient nutrient supply.					
31227153	3	67	theme	hydrogel	635:642	arg1	scaffolds					644:652	silk fibroin (SF)/gelatin composite hydrogel scaffolds	599:652	silk fibroin (SF)/gelatin composite hydrogel scaffolds	599:652	Here, bacterial cellulose nanofibers (BCNFs) were applied to improve the structural resolution and enhance mechanical properties of silk fibroin (SF)/gelatin composite hydrogel scaffolds.					
31227153	1	68	with	scaffolds	224:232	arg1	structures					250:259	biomimetic structures	239:259	biomimetic structures for tissue regeneration and organ reconstruction	239:308	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	1	69	theme	trends	145:150	arg1	trends					145:150	the latest trends	134:150	the latest trends in the regenerative medicine	134:179	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	1	69	theme	trends	145:150	arg1	One					127:129	One	127:129	One	127:129	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	1	69	theme	trends	145:150	arg1	development					188:198	the development	184:198	the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction	184:308	One of the latest trends in the regenerative medicine is the development of 3D-printing hydrogel scaffolds with biomimetic structures for tissue regeneration and organ reconstruction.					
31227153	7	70	theme	nutrient	1225:1232	arg1	supply					1234:1239	sufficient nutrient supply	1214:1239	sufficient nutrient supply	1214:1239	The pores ranging from 10 to 20 μm inside the printed filaments served as host for cellular infiltration, while the pores with a diameter from 300 to 600 μm circled by printed filaments ensured sufficient nutrient supply.					
29793112	14	0	theme	key	2450:2452	arg1	day					2420:2422	day 28	2420:2425	day 28 (p = 0.01)	2420:2436	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	14	0	theme	key	2450:2452	arg1	advantage					2454:2462	a key advantage	2448:2462	a key advantage of using the printed membranes over the AM	2448:2505	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	20	1	theme	necessary	3185:3193	arg1	characteristics					3219:3233	the necessary physical and mechanical characteristics	3181:3233	the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction	3181:3303	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	15	2	theme	layers	2620:2625	arg1	terms					2586:2590	terms	2586:2590	terms of cell morphology and cell layers	2586:2625	Histological examination showed similar qualities in the healed epithelium in terms of cell morphology and cell layers.					
29793112	14	3	theme	membrane	2386:2393	arg1	group					2395:2399	the membrane group	2382:2399	the membrane group from day 1	2382:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	1	4	theme	specific	246:253	arg1	design					275:280	a specific 3D-printed membrane design	244:280	a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction	244:377	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	20	5	theme	cell	3418:3421	arg1	density					3423:3429	higher goblet cell density	3404:3429	higher goblet cell density on the healed epithelium	3404:3454	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	20	6	theme	gelatin-based	3151:3163	arg1	membrane					3165:3172	The designed gelatin-based membrane	3138:3172	The designed gelatin-based membrane	3138:3172	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	20	7	theme	defect	3285:3290	arg1	construction					3292:3303	successful ocular surface/conjunctival defect construction	3246:3303	successful ocular surface/conjunctival defect construction	3246:3303	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	10	8	theme	cell	1575:1578	arg1	proliferation					1580:1592	goblet cell proliferation	1568:1592	goblet cell proliferation	1568:1592	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	15	9	theme	morphology	2600:2609	arg1	terms					2586:2590	terms	2586:2590	terms of cell morphology and cell layers	2586:2625	Histological examination showed similar qualities in the healed epithelium in terms of cell morphology and cell layers.					
29793112	1	10	theme	membrane	266:273	arg1	design					275:280	a specific 3D-printed membrane design	244:280	a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction	244:377	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	4	11	theme	printed	639:645	arg1	membranes					647:655	The printed membranes	635:655	The printed membranes	635:655	The printed membranes were then fully characterized in terms of physical and mechanical properties.					
29793112	10	12	theme	specific	1411:1418	arg1	staining					1458:1465	Periodic Acid Schiff staining	1437:1465	Periodic Acid Schiff staining	1437:1465	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	10	12	theme	specific	1411:1418	arg1	stains					1420:1425	specific stains	1411:1425	specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA	1411:1538	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	0	13	theme	3D-Printed	0:9	arg1	membrane					11:18	3D-Printed membrane	0:18	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.	0:141	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.					
29793112	1	14	theme	study	170:174	arg1	aim					158:160	The aim	154:160	The aim of this study	154:174	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	20	15	theme	tissue	3492:3497	arg1	formation					3499:3507	reduced scar tissue formation	3479:3507	reduced scar tissue formation	3479:3507	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	10	16	used	used	1553:1556	arg2	stains					1420:1425	specific stains	1411:1425	specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA	1411:1538	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	10	16	used	used	1553:1556	arg2	staining					1458:1465	Periodic Acid Schiff staining	1437:1465	Periodic Acid Schiff staining	1437:1465	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	14	17	from	levels	2347:2352	arg1	group					2395:2399	the membrane group	2382:2399	the membrane group from day 1	2382:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	10	18	theme	sub-epithelial	1602:1615	arg1	stroma					1617:1622	healed sub-epithelial stroma	1595:1622	healed sub-epithelial stroma	1595:1622	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	20	19	dep	CONCLUSION	3127:3136	arg1	offers					3174:3179	offers	3174:3179	offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction	3174:3303	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	20	19	dep	CONCLUSION	3127:3136	arg1	considered					3316:3325	considered	3316:3325	may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation	3309:3507	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	7	20	theme	induced	1086:1092	arg1	defects					1107:1113	induced conjunctival defects	1086:1113	induced conjunctival defects in rabbits (n = 8)	1086:1132	In vivo evaluation was conducted through implanting the gelatin-based membranes and AM on induced conjunctival defects in rabbits (n = 8).					
29793112	18	21	theme	granulomatous	2862:2874	arg1	reaction					2876:2883	granulomatous reaction	2862:2883	granulomatous reaction	2862:2883	Inflammation and granulomatous reaction was significantly higher in sections containing the AM compared to membrane (p < 0.01 and p = 0.01, respectively).					
29793112	8	22	theme	scar	1210:1213	arg1	formation					1222:1230	scar tissue formation	1210:1230	scar tissue formation	1210:1230	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	6	23	theme	membrane	954:961	arg1	handling					937:944	surgical handling	928:944	surgical handling of each membrane	928:961	Prior to in vivo experiment, surgical handling of each membrane was evaluated by three surgeons.					
29793112	20	24	theme	ocular	3257:3262	arg1	construction					3292:3303	successful ocular surface/conjunctival defect construction	3246:3303	successful ocular surface/conjunctival defect construction	3246:3303	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	11	25	theme	%	1773:1773	arg1	elastin					1779:1785	2% w/v elastin	1772:1785	2% w/v elastin	1772:1785	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	25	theme	%	1773:1773	arg1	compositions					1731:1742	all the examined compositions	1714:1742	all the examined compositions	1714:1742	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	0	26	theme	&	126:126	arg1	study					136:140	An in vitro & in vivo study	114:140	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.	0:141	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.					
29793112	15	27	theme	healed	2565:2570	arg1	epithelium					2572:2581	the healed epithelium	2561:2581	the healed epithelium in terms of cell morphology and cell layers	2561:2625	Histological examination showed similar qualities in the healed epithelium in terms of cell morphology and cell layers.					
29793112	5	28	theme	MTT	835:837	arg1	assay					839:843	MTT assay	835:843	MTT assay	835:843	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	16	29	theme	gelatin-based	2705:2717	arg1	group					2736:2740	the gelatin-based membrane grafted group	2701:2740	the gelatin-based membrane grafted group	2701:2740	However, twice the density of goblet cells per 100 cells was observed in the gelatin-based membrane grafted group.					
29793112	10	30	theme	tissue	1633:1638	arg1	formation					1640:1648	scar tissue formation	1628:1648	scar tissue formation	1628:1648	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	11	31	theme	w/v	1796:1798	arg1	compositions					1731:1742	all the examined compositions	1714:1742	all the examined compositions	1714:1742	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	31	theme	w/v	1796:1798	arg1	hyaluronate					1807:1817	0.5% w/v sodium hyaluronate	1791:1817	0.5% w/v sodium hyaluronate	1791:1817	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	13	32	theme	appropriate	2135:2145	arg1	adhesion					2152:2159	appropriate cell adhesion	2135:2159	appropriate cell adhesion	2135:2159	Epithelial cells cultivated on the membranes indicated suitable viability and proliferation, and SEM images presented appropriate cell adhesion on the surface of the membranes.					
29793112	1	33	theme	amniotic	399:406	arg1	AM					418:419	AM	418:419	AM	418:419	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	1	33	theme	amniotic	399:406	arg1	membrane					408:415	amniotic membrane	399:415	amniotic membrane (AM)	399:420	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	20	34	theme	reduced	3479:3485	arg1	formation					3499:3507	reduced scar tissue formation	3479:3507	reduced scar tissue formation	3479:3507	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	6	35	theme	surgical	928:935	arg1	handling					937:944	surgical handling	928:944	surgical handling of each membrane	928:961	Prior to in vivo experiment, surgical handling of each membrane was evaluated by three surgeons.					
29793112	16	36	theme	grafted	2728:2734	arg1	group					2736:2740	the gelatin-based membrane grafted group	2701:2740	the gelatin-based membrane grafted group	2701:2740	However, twice the density of goblet cells per 100 cells was observed in the gelatin-based membrane grafted group.					
29793112	11	37	theme	0.5	1791:1793	arg1	%					1794:1794	%	1794:1794	%	1794:1794	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	13	38	theme	suitable	2072:2079	arg1	viability					2081:2089	suitable viability	2072:2089	suitable viability	2072:2089	Epithelial cells cultivated on the membranes indicated suitable viability and proliferation, and SEM images presented appropriate cell adhesion on the surface of the membranes.					
29793112	11	39	theme	8	1756:1756	arg1	%					1757:1757	%	1757:1757	%	1757:1757	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	17	40	theme	degraded	2758:2765	arg1	implant					2767:2773	the degraded implant	2754:2773	the degraded implant	2754:2773	Remnant of the degraded implant was seen in only 3 of the membranes, but in 7 of the AM grafted eyes.					
29793112	13	41	theme	cell	2147:2150	arg1	adhesion					2152:2159	appropriate cell adhesion	2135:2159	appropriate cell adhesion	2135:2159	Epithelial cells cultivated on the membranes indicated suitable viability and proliferation, and SEM images presented appropriate cell adhesion on the surface of the membranes.					
29793112	1	42	theme	sodium	322:327	arg1	blend					341:345	sodium hyaluronate blend	322:345	sodium hyaluronate blend	322:345	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	20	43	theme	goblet	3411:3416	arg1	density					3423:3429	higher goblet cell density	3404:3429	higher goblet cell density on the healed epithelium	3404:3454	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	2	44	theme	3D	473:474	arg1	technique					485:493	3D printing technique	473:493	3D printing technique	473:493	METHODS 3D printing technique was employed to fabricate the membrane based on gradient design.					
29793112	10	45	theme	Acid	1446:1449	arg1	staining					1458:1465	Periodic Acid Schiff staining	1437:1465	Periodic Acid Schiff staining	1437:1465	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	0	46	theme	surface/conjunctival	70:89	arg1	reconstruction					98:111	ocular surface/conjunctival defect reconstruction	63:111	ocular surface/conjunctival defect reconstruction	63:111	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.					
29793112	14	47	theme	lower	2341:2345	arg1	levels					2347:2352	significantly lower levels	2327:2352	significantly lower levels of clinical inflammation in the membrane group from day 1	2327:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	20	48	theme	promising	3329:3337	arg1	alternative					3339:3349	a promising alternative	3327:3349	a promising alternative to AM due to a more predictable degradation pattern	3327:3401	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	14	49	dep	suggested	2216:2224	arg1	grafted					2310:2316	grafted	2310:2316	suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM	2216:2505	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	20	50	from	density	3423:3429	arg1	epithelium					3445:3454	the healed epithelium	3434:3454	the healed epithelium	3434:3454	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	19	51	theme	AM	3106:3107	arg1	p = 0.25					3116:3123	p = 0.25	3116:3123	p = 0.25	3116:3123	α-SMA staining was more evident, but not significantly different from the gelatin-based membrane, for the AM group (p = 0.25).					
29793112	19	51	theme	AM	3106:3107	arg1	group					3109:3113	the AM group	3102:3113	the AM group (p = 0.25)	3102:3124	α-SMA staining was more evident, but not significantly different from the gelatin-based membrane, for the AM group (p = 0.25).					
29793112	11	52	theme	w/v	1759:1761	arg1	gelatin					1763:1769	8% w/v gelatin	1756:1769	8% w/v gelatin	1756:1769	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	1	53	theme	conjunctival	351:362	arg1	reconstruction					364:377	conjunctival reconstruction	351:377	conjunctival reconstruction	351:377	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	12	54	theme	optical	1897:1903	arg1	characteristics					1905:1919	favorable optical characteristics	1887:1919	favorable optical characteristics (colorless and transparent)	1887:1947	The printed membranes had favorable optical characteristics (colorless and transparent), and the surgical handling was significantly easier compared to AM.					
29793112	5	55	theme	In vitro	735:742	arg1	viability					744:752	In vitro viability	735:752	In vitro viability	735:752	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	20	56	theme	higher	3404:3409	arg1	density					3423:3429	higher goblet cell density	3404:3429	higher goblet cell density on the healed epithelium	3404:3454	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	4	57	theme	properties	723:732	arg1	terms					690:694	terms	690:694	terms of physical and mechanical properties	690:732	The printed membranes were then fully characterized in terms of physical and mechanical properties.					
29793112	17	58	theme	grafted	2831:2837	arg1	eyes					2839:2842	the AM grafted eyes	2824:2842	the AM grafted eyes	2824:2842	Remnant of the degraded implant was seen in only 3 of the membranes, but in 7 of the AM grafted eyes.					
29793112	5	59	theme	cells	809:813	arg1	adhesion					773:780	adhesion	773:780	adhesion	773:780	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	5	59	theme	cells	809:813	arg1	proliferation					755:767	proliferation	755:767	proliferation	755:767	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	5	59	theme	cells	809:813	arg1	viability					744:752	In vitro viability	735:752	In vitro viability	735:752	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	1	60	theme	design	275:280	arg1	applicability					227:239	clinical applicability	218:239	clinical applicability	218:239	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	1	60	theme	design	275:280	arg1	handling					205:212	the surgical handling	192:212	the surgical handling	192:212	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	12	61	theme	printed	1865:1871	arg1	membranes					1873:1881	The printed membranes	1861:1881	The printed membranes	1861:1881	The printed membranes had favorable optical characteristics (colorless and transparent), and the surgical handling was significantly easier compared to AM.					
29793112	19	62	from	membrane	3088:3095	arg1	different					3055:3063	different	3055:3063	different	3055:3063	α-SMA staining was more evident, but not significantly different from the gelatin-based membrane, for the AM group (p = 0.25).					
29793112	16	63	theme	cells	2665:2669	arg1	density					2647:2653	twice the density	2637:2653	twice the density of goblet cells per 100 cells	2637:2683	However, twice the density of goblet cells per 100 cells was observed in the gelatin-based membrane grafted group.					
29793112	1	64	dep	BACKGROUND	143:152	arg1	was					176:178	was	176:178	was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries	176:462	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	20	65	theme	predictable	3371:3381	arg1	pattern					3395:3401	a more predictable degradation pattern	3364:3401	a more predictable degradation pattern	3364:3401	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	7	66	from	defects	1107:1113	arg1	rabbits					1118:1124	rabbits	1118:1124	rabbits (n = 8)	1118:1132	In vivo evaluation was conducted through implanting the gelatin-based membranes and AM on induced conjunctival defects in rabbits (n = 8).					
29793112	7	66	from	defects	1107:1113	arg1	n = 8					1127:1131	n = 8	1127:1131	n = 8	1127:1131	In vivo evaluation was conducted through implanting the gelatin-based membranes and AM on induced conjunctival defects in rabbits (n = 8).					
29793112	10	67	theme	Trichrome	1477:1485	arg1	staining					1487:1494	Masson's Trichrome staining	1468:1494	Masson's Trichrome staining	1468:1494	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	12	68	contain	had	1883:1885	arg2	characteristics					1905:1919	favorable optical characteristics	1887:1919	favorable optical characteristics (colorless and transparent)	1887:1947	The printed membranes had favorable optical characteristics (colorless and transparent), and the surgical handling was significantly easier compared to AM.					
29793112	12	68	contain	had	1883:1885	arg1	membranes					1873:1881	The printed membranes	1861:1881	The printed membranes	1861:1881	The printed membranes had favorable optical characteristics (colorless and transparent), and the surgical handling was significantly easier compared to AM.					
29793112	8	69	theme	granulation	1248:1258	arg1	tissue					1260:1265	granulation tissue	1248:1265	granulation tissue	1248:1265	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	20	70	theme	mechanical	3208:3217	arg1	characteristics					3219:3233	the necessary physical and mechanical characteristics	3181:3233	the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction	3181:3303	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	3	71	theme	ink	618:620	arg1	composition					622:632	the ink composition	614:632	the ink composition	614:632	Prior to printing, rheometry was employed to optimize the ink composition.					
29793112	5	72	theme	limbal	791:796	arg1	cells					809:813	human limbal epithelial cells	785:813	human limbal epithelial cells	785:813	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	2	73	dep	METHODS	465:471	arg1	employed					499:506	employed	499:506	was employed to fabricate the membrane based on gradient design	495:557	METHODS 3D printing technique was employed to fabricate the membrane based on gradient design.					
29793112	14	74	theme	printed	2477:2483	arg1	membranes					2485:2493	the printed membranes	2473:2493	the printed membranes	2473:2493	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	14	75	dep	membrane	2294:2301	arg1	the					2290:2292	the	2290:2292	the	2290:2292	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	14	76	theme	clinical	2357:2364	arg1	inflammation					2366:2377	clinical inflammation	2357:2377	clinical inflammation in the membrane group from day 1	2357:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	17	77	theme	membranes	2801:2809	arg1	membranes					2801:2809	the membranes	2797:2809	the membranes	2797:2809	Remnant of the degraded implant was seen in only 3 of the membranes, but in 7 of the AM grafted eyes.					
29793112	17	77	theme	membranes	2801:2809	arg1	3					2792:2792	3	2792:2792	3	2792:2792	Remnant of the degraded implant was seen in only 3 of the membranes, but in 7 of the AM grafted eyes.					
29793112	16	78	located	observed	2689:2696	arg2	density					2647:2653	twice the density	2637:2653	twice the density of goblet cells per 100 cells	2637:2683	However, twice the density of goblet cells per 100 cells was observed in the gelatin-based membrane grafted group.					
29793112	16	78	located	observed	2689:2696	arg1	group					2736:2740	the gelatin-based membrane grafted group	2701:2740	the gelatin-based membrane grafted group	2701:2740	However, twice the density of goblet cells per 100 cells was observed in the gelatin-based membrane grafted group.					
29793112	14	79	theme	epithelialization	2234:2250	arg1	time					2252:2255	similar epithelialization time	2226:2255	similar epithelialization time (approximately 3 weeks) for both the membrane and AM	2226:2308	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	14	79	theme	epithelialization	2234:2250	arg1	weeks					2274:2278	approximately 3 weeks	2258:2278	approximately 3 weeks	2258:2278	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	5	80	theme	electron	858:865	arg1	SEM					879:881	SEM	879:881	SEM	879:881	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	5	80	theme	electron	858:865	arg1	microscopy					867:876	scanning electron microscopy	849:876	scanning electron microscopy (SEM)	849:882	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	6	81	theme	in vivo	908:914	arg1	experiment					916:925	in vivo experiment	908:925	in vivo experiment	908:925	Prior to in vivo experiment, surgical handling of each membrane was evaluated by three surgeons.					
29793112	20	82	theme	physical	3195:3202	arg1	characteristics					3219:3233	the necessary physical and mechanical characteristics	3181:3233	the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction	3181:3303	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	17	83	theme	implant	2767:2773	arg1	Remnant					2743:2749	Remnant	2743:2749	Remnant of the degraded implant	2743:2773	Remnant of the degraded implant was seen in only 3 of the membranes, but in 7 of the AM grafted eyes.					
29793112	8	84	theme	Clinical	1135:1142	arg1	formation					1222:1230	scar tissue formation	1210:1230	scar tissue formation	1210:1230	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	8	84	theme	Clinical	1135:1142	arg1	observations					1144:1155	Clinical observations	1135:1155	Clinical observations	1135:1155	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	8	84	theme	Clinical	1135:1142	arg1	epithelialization					1168:1184	epithelialization	1168:1184	epithelialization	1168:1184	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	8	84	theme	Clinical	1135:1142	arg1	severity					1200:1207	inflammation severity	1187:1207	inflammation severity	1187:1207	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	8	84	theme	Clinical	1135:1142	arg1	presence					1236:1243	presence	1236:1243	presence	1236:1243	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	14	85	theme	Clinical	2194:2201	arg1	observations					2203:2214	Clinical observations	2194:2214	Clinical observations	2194:2214	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	13	86	attach	presented	2125:2133	arg2	images					2118:2123	SEM images	2114:2123	SEM images	2114:2123	Epithelial cells cultivated on the membranes indicated suitable viability and proliferation, and SEM images presented appropriate cell adhesion on the surface of the membranes.					
29793112	13	86	attach	presented	2125:2133	arg1	surface					2168:2174	the surface	2164:2174	the surface of the membranes	2164:2191	Epithelial cells cultivated on the membranes indicated suitable viability and proliferation, and SEM images presented appropriate cell adhesion on the surface of the membranes.					
29793112	15	87	theme	Histological	2508:2519	arg1	examination					2521:2531	Histological examination	2508:2531	Histological examination	2508:2531	Histological examination showed similar qualities in the healed epithelium in terms of cell morphology and cell layers.					
29793112	1	88	theme	3D-printed	255:264	arg1	design					275:280	a specific 3D-printed membrane design	244:280	a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction	244:377	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	12	89	theme	surgical	1958:1965	arg1	handling					1967:1974	the surgical handling	1954:1974	the surgical handling	1954:1974	The printed membranes had favorable optical characteristics (colorless and transparent), and the surgical handling was significantly easier compared to AM.					
29793112	12	89	theme	surgical	1958:1965	arg1	easier					1994:1999	easier	1994:1999	easier	1994:1999	The printed membranes had favorable optical characteristics (colorless and transparent), and the surgical handling was significantly easier compared to AM.					
29793112	14	90	from	inflammation	2366:2377	arg1	day					2406:2408	day 1	2406:2410	day 1	2406:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	14	90	from	inflammation	2366:2377	arg1	group					2395:2399	the membrane group	2382:2399	the membrane group from day 1	2382:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	10	91	theme	goblet	1568:1573	arg1	proliferation					1580:1592	goblet cell proliferation	1568:1592	goblet cell proliferation	1568:1592	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	7	92	theme	conjunctival	1094:1105	arg1	defects					1107:1113	induced conjunctival defects	1086:1113	induced conjunctival defects in rabbits (n = 8)	1086:1132	In vivo evaluation was conducted through implanting the gelatin-based membranes and AM on induced conjunctival defects in rabbits (n = 8).					
29793112	14	93	theme	inflammation	2366:2377	arg1	levels					2347:2352	significantly lower levels	2327:2352	significantly lower levels of clinical inflammation in the membrane group from day 1	2327:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	5	94	theme	scanning	849:856	arg1	SEM					879:881	SEM	879:881	SEM	879:881	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	5	94	theme	scanning	849:856	arg1	microscopy					867:876	scanning electron microscopy	849:876	scanning electron microscopy (SEM)	849:882	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	0	95	theme	amniotic	41:48	arg1	membrane					50:57	amniotic membrane	41:57	amniotic membrane	41:57	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.					
29793112	20	96	theme	successful	3246:3255	arg1	construction					3292:3303	successful ocular surface/conjunctival defect construction	3246:3303	successful ocular surface/conjunctival defect construction	3246:3303	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	20	97	theme	scar	3487:3490	arg1	formation					3499:3507	reduced scar tissue formation	3479:3507	reduced scar tissue formation	3479:3507	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	1	98	theme	such	449:452	arg1	surgeries					454:462	such surgeries	449:462	such surgeries	449:462	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	15	99	theme	cell	2595:2598	arg1	morphology					2600:2609	cell morphology	2595:2609	cell morphology	2595:2609	Histological examination showed similar qualities in the healed epithelium in terms of cell morphology and cell layers.					
29793112	8	100	theme	inflammation	1187:1198	arg1	severity					1200:1207	inflammation severity	1187:1207	inflammation severity	1187:1207	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	20	101	theme	surface/conjunctival	3264:3283	arg1	construction					3292:3303	successful ocular surface/conjunctival defect construction	3246:3303	successful ocular surface/conjunctival defect construction	3246:3303	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	8	102	theme	tissue	1260:1265	arg1	formation					1222:1230	scar tissue formation	1210:1230	scar tissue formation	1210:1230	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	8	102	theme	tissue	1260:1265	arg1	epithelialization					1168:1184	epithelialization	1168:1184	epithelialization	1168:1184	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	8	102	theme	tissue	1260:1265	arg1	severity					1200:1207	inflammation severity	1187:1207	inflammation severity	1187:1207	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	8	102	theme	tissue	1260:1265	arg1	presence					1236:1243	presence	1236:1243	presence	1236:1243	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	10	103	theme	healed	1595:1600	arg1	stroma					1617:1622	healed sub-epithelial stroma	1595:1622	healed sub-epithelial stroma	1595:1622	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	0	104	theme	in vivo	128:134	arg1	study					136:140	An in vitro & in vivo study	114:140	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.	0:141	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.					
29793112	10	105	theme	H&E	1397:1399	arg1	staining					1401:1408	H&E staining	1397:1408	H&E staining	1397:1408	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	12	106	dep	characteristics	1905:1919	arg1	transparent					1936:1946	transparent	1936:1946	transparent	1936:1946	The printed membranes had favorable optical characteristics (colorless and transparent), and the surgical handling was significantly easier compared to AM.					
29793112	12	106	dep	characteristics	1905:1919	arg1	colorless					1922:1930	colorless	1922:1930	colorless	1922:1930	The printed membranes had favorable optical characteristics (colorless and transparent), and the surgical handling was significantly easier compared to AM.					
29793112	11	107	theme	w/v	1775:1777	arg1	elastin					1779:1785	2% w/v elastin	1772:1785	2% w/v elastin	1772:1785	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	107	theme	w/v	1775:1777	arg1	compositions					1731:1742	all the examined compositions	1714:1742	all the examined compositions	1714:1742	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	7	108	theme	gelatin-based	1052:1064	arg1	membranes					1066:1074	the gelatin-based membranes	1048:1074	the gelatin-based membranes	1048:1074	In vivo evaluation was conducted through implanting the gelatin-based membranes and AM on induced conjunctival defects in rabbits (n = 8).					
29793112	1	109	theme	surgical	196:203	arg1	handling					205:212	the surgical handling	192:212	the surgical handling	192:212	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	8	110	theme	tissue	1215:1220	arg1	formation					1222:1230	scar tissue formation	1210:1230	scar tissue formation	1210:1230	Clinical observations, including epithelialization, inflammation severity, scar tissue formation and presence of granulation tissue, were recorded from day 1 through day 28.					
29793112	10	111	theme	scar	1628:1631	arg1	formation					1640:1648	scar tissue formation	1628:1648	scar tissue formation	1628:1648	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	11	112	theme	sodium	1800:1805	arg1	compositions					1731:1742	all the examined compositions	1714:1742	all the examined compositions	1714:1742	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	112	theme	sodium	1800:1805	arg1	hyaluronate					1807:1817	0.5% w/v sodium hyaluronate	1791:1817	0.5% w/v sodium hyaluronate	1791:1817	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	16	113	theme	membrane	2719:2726	arg1	group					2736:2740	the gelatin-based membrane grafted group	2701:2740	the gelatin-based membrane grafted group	2701:2740	However, twice the density of goblet cells per 100 cells was observed in the gelatin-based membrane grafted group.					
29793112	11	114	theme	%	1794:1794	arg1	compositions					1731:1742	all the examined compositions	1714:1742	all the examined compositions	1714:1742	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	114	theme	%	1794:1794	arg1	hyaluronate					1807:1817	0.5% w/v sodium hyaluronate	1791:1817	0.5% w/v sodium hyaluronate	1791:1817	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	7	115	theme	In vivo	996:1002	arg1	evaluation					1004:1013	In vivo evaluation	996:1013	In vivo evaluation	996:1013	In vivo evaluation was conducted through implanting the gelatin-based membranes and AM on induced conjunctival defects in rabbits (n = 8).					
29793112	2	116	theme	gradient	543:550	arg1	design					552:557	gradient design	543:557	gradient design	543:557	METHODS 3D printing technique was employed to fabricate the membrane based on gradient design.					
29793112	14	117	from	group	2395:2399	arg1	levels					2347:2352	significantly lower levels	2327:2352	significantly lower levels of clinical inflammation in the membrane group from day 1	2327:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	4	118	theme	physical	699:706	arg1	properties					723:732	physical and mechanical properties	699:732	physical and mechanical properties	699:732	The printed membranes were then fully characterized in terms of physical and mechanical properties.					
29793112	19	119	theme	gelatin-based	3074:3086	arg1	membrane					3088:3095	the gelatin-based membrane	3070:3095	the gelatin-based membrane	3070:3095	α-SMA staining was more evident, but not significantly different from the gelatin-based membrane, for the AM group (p = 0.25).					
29793112	11	120	theme	%	1757:1757	arg1	gelatin					1763:1769	8% w/v gelatin	1756:1769	8% w/v gelatin	1756:1769	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	0	121	theme	ocular	63:68	arg1	reconstruction					98:111	ocular surface/conjunctival defect reconstruction	63:111	ocular surface/conjunctival defect reconstruction	63:111	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.					
29793112	2	122	theme	printing	476:483	arg1	technique					485:493	3D printing technique	473:493	3D printing technique	473:493	METHODS 3D printing technique was employed to fabricate the membrane based on gradient design.					
29793112	14	123	from	day	2406:2408	arg1	group					2395:2399	the membrane group	2382:2399	the membrane group from day 1	2382:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	14	123	from	day	2406:2408	arg1	inflammation					2366:2377	clinical inflammation	2357:2377	clinical inflammation in the membrane group from day 1	2357:2410	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	0	124	theme	defect	91:96	arg1	reconstruction					98:111	ocular surface/conjunctival defect reconstruction	63:111	ocular surface/conjunctival defect reconstruction	63:111	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.					
29793112	1	125	theme	hyaluronate	329:339	arg1	blend					341:345	sodium hyaluronate blend	322:345	sodium hyaluronate blend	322:345	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	12	126	theme	favorable	1887:1895	arg1	characteristics					1905:1919	favorable optical characteristics	1887:1919	favorable optical characteristics (colorless and transparent)	1887:1947	The printed membranes had favorable optical characteristics (colorless and transparent), and the surgical handling was significantly easier compared to AM.					
29793112	10	127	theme	Periodic	1437:1444	arg1	staining					1458:1465	Periodic Acid Schiff staining	1437:1465	Periodic Acid Schiff staining	1437:1465	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	11	128	theme	2	1772:1772	arg1	%					1773:1773	%	1773:1773	%	1773:1773	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	9	129	from	eyes	1366:1369	arg1	day					1374:1376	day 28	1374:1379	day 28	1374:1379	Histological examination was performed on all enucleated eyes on day 28.					
29793112	18	130	dep	compared	2940:2947	arg1	p < 0.01					2962:2969	p < 0.01	2962:2969	p < 0.01	2962:2969	Inflammation and granulomatous reaction was significantly higher in sections containing the AM compared to membrane (p < 0.01 and p = 0.01, respectively).					
29793112	18	130	dep	compared	2940:2947	arg1	AM					2937:2938	the AM	2933:2938	the AM compared to membrane (p < 0.01 and p = 0.01, respectively)	2933:2997	Inflammation and granulomatous reaction was significantly higher in sections containing the AM compared to membrane (p < 0.01 and p = 0.01, respectively).					
29793112	18	130	dep	compared	2940:2947	arg1	p = 0.01					2975:2982	p = 0.01	2975:2982	p = 0.01	2975:2982	Inflammation and granulomatous reaction was significantly higher in sections containing the AM compared to membrane (p < 0.01 and p = 0.01, respectively).					
29793112	11	131	theme	gelatin	1763:1769	arg1	elastin					1779:1785	2% w/v elastin	1772:1785	2% w/v elastin	1772:1785	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	131	theme	gelatin	1763:1769	arg1	compositions					1731:1742	all the examined compositions	1714:1742	all the examined compositions	1714:1742	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	131	theme	gelatin	1763:1769	arg1	blend					1747:1751	a blend	1745:1751	a blend of 8% w/v gelatin	1745:1769	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	131	theme	gelatin	1763:1769	arg1	hyaluronate					1807:1817	0.5% w/v sodium hyaluronate	1791:1817	0.5% w/v sodium hyaluronate	1791:1817	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	0	132	theme	in vitro	117:124	arg1	study					136:140	An in vitro & in vivo study	114:140	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.	0:141	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.					
29793112	9	133	theme	Histological	1309:1320	arg1	examination					1322:1332	Histological examination	1309:1332	Histological examination	1309:1332	Histological examination was performed on all enucleated eyes on day 28.					
29793112	20	134	theme	degradation	3383:3393	arg1	pattern					3395:3401	a more predictable degradation pattern	3364:3401	a more predictable degradation pattern	3364:3401	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	16	135	theme	goblet	2658:2663	arg1	cells					2665:2669	goblet cells	2658:2669	goblet cells	2658:2669	However, twice the density of goblet cells per 100 cells was observed in the gelatin-based membrane grafted group.					
29793112	1	136	dep	gelatin	301:307	arg1	a					299:299	a	299:299	a	299:299	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	15	137	theme	cell	2615:2618	arg1	layers					2620:2625	cell layers	2615:2625	cell layers	2615:2625	Histological examination showed similar qualities in the healed epithelium in terms of cell morphology and cell layers.					
29793112	5	138	theme	epithelial	798:807	arg1	cells					809:813	human limbal epithelial cells	785:813	human limbal epithelial cells	785:813	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	13	139	theme	Epithelial	2017:2026	arg1	cells					2028:2032	Epithelial cells	2017:2032	Epithelial cells cultivated on the membranes	2017:2060	Epithelial cells cultivated on the membranes indicated suitable viability and proliferation, and SEM images presented appropriate cell adhesion on the surface of the membranes.					
29793112	10	140	theme	Schiff	1451:1456	arg1	staining					1458:1465	Periodic Acid Schiff staining	1437:1465	Periodic Acid Schiff staining	1437:1465	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	4	141	theme	mechanical	712:721	arg1	properties					723:732	physical and mechanical properties	699:732	physical and mechanical properties	699:732	The printed membranes were then fully characterized in terms of physical and mechanical properties.					
29793112	17	142	theme	eyes	2839:2842	arg1	eyes					2839:2842	the AM grafted eyes	2824:2842	the AM grafted eyes	2824:2842	Remnant of the degraded implant was seen in only 3 of the membranes, but in 7 of the AM grafted eyes.					
29793112	17	142	theme	eyes	2839:2842	arg1	7					2819:2819	7	2819:2819	7	2819:2819	Remnant of the degraded implant was seen in only 3 of the membranes, but in 7 of the AM grafted eyes.					
29793112	18	143	contain	containing	2922:2931	arg2	p = 0.01					2975:2982	p = 0.01	2975:2982	p = 0.01	2975:2982	Inflammation and granulomatous reaction was significantly higher in sections containing the AM compared to membrane (p < 0.01 and p = 0.01, respectively).					
29793112	18	143	contain	containing	2922:2931	arg1	sections					2913:2920	sections	2913:2920	sections containing the AM compared to membrane (p < 0.01 and p = 0.01, respectively)	2913:2997	Inflammation and granulomatous reaction was significantly higher in sections containing the AM compared to membrane (p < 0.01 and p = 0.01, respectively).					
29793112	18	143	contain	containing	2922:2931	arg2	p < 0.01					2962:2969	p < 0.01	2962:2969	p < 0.01	2962:2969	Inflammation and granulomatous reaction was significantly higher in sections containing the AM compared to membrane (p < 0.01 and p = 0.01, respectively).					
29793112	18	143	contain	containing	2922:2931	arg2	AM					2937:2938	the AM	2933:2938	the AM compared to membrane (p < 0.01 and p = 0.01, respectively)	2933:2997	Inflammation and granulomatous reaction was significantly higher in sections containing the AM compared to membrane (p < 0.01 and p = 0.01, respectively).					
29793112	9	144	theme	enucleated	1355:1364	arg1	eyes					1366:1369	all enucleated eyes	1351:1369	all enucleated eyes on day 28	1351:1379	Histological examination was performed on all enucleated eyes on day 28.					
29793112	15	145	from	epithelium	2572:2581	arg1	terms					2586:2590	terms	2586:2590	terms of cell morphology and cell layers	2586:2625	Histological examination showed similar qualities in the healed epithelium in terms of cell morphology and cell layers.					
29793112	10	146	theme	myofibroblasts	1670:1683	arg1	presence					1658:1665	the presence	1654:1665	the presence of myofibroblasts	1654:1683	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	10	146	theme	myofibroblasts	1670:1683	arg1	stroma					1617:1622	healed sub-epithelial stroma	1595:1622	healed sub-epithelial stroma	1595:1622	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	10	146	theme	myofibroblasts	1670:1683	arg1	formation					1640:1648	scar tissue formation	1628:1648	scar tissue formation	1628:1648	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	10	146	theme	myofibroblasts	1670:1683	arg1	proliferation					1580:1592	goblet cell proliferation	1568:1592	goblet cell proliferation	1568:1592	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	5	147	theme	human	785:789	arg1	cells					809:813	human limbal epithelial cells	785:813	human limbal epithelial cells	785:813	In vitro viability, proliferation and adhesion of human limbal epithelial cells were assessed using MTT assay and scanning electron microscopy (SEM), respectively.					
29793112	13	148	theme	membranes	2183:2191	arg1	surface					2168:2174	the surface	2164:2174	the surface of the membranes	2164:2191	Epithelial cells cultivated on the membranes indicated suitable viability and proliferation, and SEM images presented appropriate cell adhesion on the surface of the membranes.					
29793112	20	149	theme	designed	3142:3149	arg1	membrane					3165:3172	The designed gelatin-based membrane	3138:3172	The designed gelatin-based membrane	3138:3172	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	13	150	theme	SEM	2114:2116	arg1	images					2118:2123	SEM images	2114:2123	SEM images	2114:2123	Epithelial cells cultivated on the membranes indicated suitable viability and proliferation, and SEM images presented appropriate cell adhesion on the surface of the membranes.					
29793112	10	151	theme	immuno-histochemical	1500:1519	arg1	staining					1521:1528	immuno-histochemical staining	1500:1528	immuno-histochemical staining	1500:1528	In addition to H&E staining, specific stains including Periodic Acid Schiff staining, Masson's Trichrome staining and immuno-histochemical staining for α-SMA were further used to assess goblet cell proliferation, healed sub-epithelial stroma and scar tissue formation and the presence of myofibroblasts, respectively.					
29793112	11	152	theme	examined	1722:1729	arg1	elastin					1779:1785	2% w/v elastin	1772:1785	2% w/v elastin	1772:1785	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	152	theme	examined	1722:1729	arg1	compositions					1731:1742	all the examined compositions	1714:1742	all the examined compositions	1714:1742	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	152	theme	examined	1722:1729	arg1	blend					1747:1751	a blend	1745:1751	a blend of 8% w/v gelatin	1745:1769	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	11	152	theme	examined	1722:1729	arg1	hyaluronate					1807:1817	0.5% w/v sodium hyaluronate	1791:1817	0.5% w/v sodium hyaluronate	1791:1817	RESULTS Among all the examined compositions, a blend of 8% w/v gelatin, 2% w/v elastin and 0.5% w/v sodium hyaluronate was found to be appropriate for printing.					
29793112	15	153	theme	similar	2540:2546	arg1	qualities					2548:2556	similar qualities	2540:2556	similar qualities	2540:2556	Histological examination showed similar qualities in the healed epithelium in terms of cell morphology and cell layers.					
29793112	1	154	theme	clinical	218:225	arg1	applicability					227:239	clinical applicability	218:239	clinical applicability	218:239	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	20	155	theme	healed	3438:3443	arg1	epithelium					3445:3454	the healed epithelium	3434:3454	the healed epithelium	3434:3454	CONCLUSION The designed gelatin-based membrane offers the necessary physical and mechanical characteristics needed for successful ocular surface/conjunctival defect construction and may be considered a promising alternative to AM due to a more predictable degradation pattern, higher goblet cell density on the healed epithelium, less inflammation and reduced scar tissue formation.					
29793112	0	156	dep	membrane	11:18	arg1	study					136:140	An in vitro & in vivo study	114:140	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.	0:141	3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study.					
29793112	14	157	theme	similar	2226:2232	arg1	time					2252:2255	similar epithelialization time	2226:2255	similar epithelialization time (approximately 3 weeks) for both the membrane and AM	2226:2308	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	14	157	theme	similar	2226:2232	arg1	weeks					2274:2278	approximately 3 weeks	2258:2278	approximately 3 weeks	2258:2278	Clinical observations suggested similar epithelialization time (approximately 3 weeks) for both the membrane and AM grafted eyes but significantly lower levels of clinical inflammation in the membrane group from day 1 through day 28 (p = 0.01), which is a key advantage of using the printed membranes over the AM.					
29793112	19	158	theme	α-SMA	3000:3004	arg1	staining					3006:3013	α-SMA staining	3000:3013	α-SMA staining	3000:3013	α-SMA staining was more evident, but not significantly different from the gelatin-based membrane, for the AM group (p = 0.25).					
29793112	1	159	used	used	441:444	arg2	AM					418:419	AM	418:419	AM	418:419	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29793112	1	159	used	used	441:444	arg2	membrane					408:415	amniotic membrane	399:415	amniotic membrane (AM)	399:420	BACKGROUND The aim of this study was to evaluate the surgical handling and clinical applicability of a specific 3D-printed membrane design fabricated using a gelatin, elastin and sodium hyaluronate blend for conjunctival reconstruction and compare it with amniotic membrane (AM), which is normally used in such surgeries.					
29391228	4	0	dep	Cht	512:514	arg1	St-AgNPs					516:523	St-AgNPs	516:523	Cht:St-AgNPs	512:523	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	0	1	theme	functional	15:24	arg1	papers					40:45	functional antimicrobial papers	15:45	functional antimicrobial papers	15:45	Development of functional antimicrobial papers using chitosan/starch-silver nanoparticles.					
29391228	4	2	theme	different	492:500	arg1	ratios					502:507	different ratios	492:507	different ratios of Cht:St-AgNPs	492:523	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	3	theme	paper	540:544	arg1	properties					576:585	mechanical properties	565:585	mechanical properties	565:585	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	3	theme	paper	540:544	arg1	activities					632:641	antimicrobial activities	618:641	antimicrobial activities	618:641	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	3	theme	paper	540:544	arg1	properties					546:555	the various paper properties	528:555	the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities	528:641	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	3	theme	paper	540:544	arg1	water					588:592	water	588:592	water	588:592	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	7	4	theme	packaging	1159:1167	arg1	applications					1169:1180	functional antimicrobial packaging applications	1134:1180	functional antimicrobial packaging applications	1134:1180	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	6	5	theme	mechanical	856:865	arg1	properties					867:876	excellent mechanical properties	846:876	excellent mechanical properties	846:876	The Cht:St-AgNPs-coated paper prepared with the ratio of 9:1 showed excellent mechanical properties and good resistance properties against water and oil.					
29391228	4	6	from	influence	479:487	arg1	properties					576:585	mechanical properties	565:585	mechanical properties	565:585	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	6	from	influence	479:487	arg1	activities					632:641	antimicrobial activities	618:641	antimicrobial activities	618:641	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	6	from	influence	479:487	arg1	properties					546:555	the various paper properties	528:555	the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities	528:641	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	6	from	influence	479:487	arg1	water					588:592	water	588:592	water	588:592	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	7	7	theme	antimicrobial	1145:1157	arg1	applications					1169:1180	functional antimicrobial packaging applications	1134:1180	functional antimicrobial packaging applications	1134:1180	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	6	8	theme	excellent	846:854	arg1	properties					867:876	excellent mechanical properties	846:876	excellent mechanical properties	846:876	The Cht:St-AgNPs-coated paper prepared with the ratio of 9:1 showed excellent mechanical properties and good resistance properties against water and oil.					
29391228	7	9	theme	antifungal	1067:1076	arg1	activity					1078:1085	antifungal activity	1067:1085	antifungal activity	1067:1085	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	7	10	theme	coated	949:954	arg1	papers					956:961	St-AgNPs coated papers	940:961	St-AgNPs coated papers	940:961	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	7	11	theme	mechanical	998:1007	arg1	strength					1009:1016	mechanical strength	998:1016	mechanical strength	998:1016	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	5	12	theme	coated	704:709	arg1	papers					711:716	the coated papers	700:716	the coated papers	700:716	It was observed that the properties of the coated papers were strongly dependent on the composition of Cht:St-AgNPs.					
29391228	7	13	theme	potential	1110:1118	arg1	candidate					1120:1128	a potential candidate	1108:1128	a potential candidate for functional antimicrobial packaging applications	1108:1180	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	4	14	theme	antimicrobial	618:630	arg1	activities					632:641	antimicrobial activities	618:641	antimicrobial activities	618:641	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	5	15	theme	papers	711:716	arg1	dependent					732:740	dependent	732:740	dependent	732:740	It was observed that the properties of the coated papers were strongly dependent on the composition of Cht:St-AgNPs.					
29391228	5	15	theme	papers	711:716	arg1	properties					686:695	the properties	682:695	the properties of the coated papers	682:716	It was observed that the properties of the coated papers were strongly dependent on the composition of Cht:St-AgNPs.					
29391228	6	16	theme	9:1	835:837	arg1	ratio					826:830	the ratio	822:830	the ratio of 9:1	822:837	The Cht:St-AgNPs-coated paper prepared with the ratio of 9:1 showed excellent mechanical properties and good resistance properties against water and oil.					
29391228	1	17	theme	present	98:104	arg1	work					106:109	the present work	94:109	the present work	94:109	In the present work, we report the synthesis of chitosan:starch‑silver nanoparticle (Cht:St-AgNPs) coated papers for antimicrobial packaging applications.					
29391228	3	18	theme	7:3	442:444	arg1	ratios					422:427	different ratios	412:427	different ratios of 9:1, 8:2, 7:3, and 5:5 by weight	412:463	Chitosan was mixed into the synthesized St-AgNPs solution with different ratios of 9:1, 8:2, 7:3, and 5:5 by weight.					
29391228	7	19	dep	make	1098:1101	arg1	can					1094:1096	can	1094:1096	can	1094:1096	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	1	20	dep	nanoparticle	162:173	arg1	Cht					176:178	Cht	176:178	Cht:St-AgNPs	176:187	In the present work, we report the synthesis of chitosan:starch‑silver nanoparticle (Cht:St-AgNPs) coated papers for antimicrobial packaging applications.					
29391228	7	21	from	enhancement	983:993	arg1	oil					1019:1021	oil	1019:1021	oil	1019:1021	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	7	21	from	enhancement	983:993	arg1	antibacterial					1049:1061	antibacterial	1049:1061	antibacterial	1049:1061	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	7	21	from	enhancement	983:993	arg1	strength					1009:1016	mechanical strength	998:1016	mechanical strength	998:1016	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	6	22	theme	resistance	887:896	arg1	properties					898:907	good resistance properties	882:907	good resistance properties	882:907	The Cht:St-AgNPs-coated paper prepared with the ratio of 9:1 showed excellent mechanical properties and good resistance properties against water and oil.					
29391228	2	23	theme	particle	326:333	arg1	size					335:338	an average particle size	315:338	an average particle size of 7 nm	315:346	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	7	24	dep	Cht	936:938	arg1	showed					963:968	showed	963:968	showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications	963:1180	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	3	25	theme	8:2	437:439	arg1	ratios					422:427	different ratios	412:427	different ratios of 9:1, 8:2, 7:3, and 5:5 by weight	412:463	Chitosan was mixed into the synthesized St-AgNPs solution with different ratios of 9:1, 8:2, 7:3, and 5:5 by weight.					
29391228	0	26	theme	antimicrobial	26:38	arg1	papers					40:45	functional antimicrobial papers	15:45	functional antimicrobial papers	15:45	Development of functional antimicrobial papers using chitosan/starch-silver nanoparticles.					
29391228	6	27	dep	Cht	782:784	arg1	paper					802:806	St-AgNPs-coated paper	786:806	The Cht:St-AgNPs-coated paper prepared with the ratio of 9:1	778:837	The Cht:St-AgNPs-coated paper prepared with the ratio of 9:1 showed excellent mechanical properties and good resistance properties against water and oil.					
29391228	2	28	theme	average	318:324	arg1	size					335:338	an average particle size	315:338	an average particle size of 7 nm	315:346	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	3	29	theme	5:5	451:453	arg1	ratios					422:427	different ratios	412:427	different ratios of 9:1, 8:2, 7:3, and 5:5 by weight	412:463	Chitosan was mixed into the synthesized St-AgNPs solution with different ratios of 9:1, 8:2, 7:3, and 5:5 by weight.					
29391228	3	30	theme	St-AgNPs	389:396	arg1	solution					398:405	the synthesized St-AgNPs solution	373:405	the synthesized St-AgNPs solution	373:405	Chitosan was mixed into the synthesized St-AgNPs solution with different ratios of 9:1, 8:2, 7:3, and 5:5 by weight.					
29391228	4	31	theme	various	532:538	arg1	properties					576:585	mechanical properties	565:585	mechanical properties	565:585	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	31	theme	various	532:538	arg1	activities					632:641	antimicrobial activities	618:641	antimicrobial activities	618:641	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	31	theme	various	532:538	arg1	properties					546:555	the various paper properties	528:555	the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities	528:641	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	4	31	theme	various	532:538	arg1	water					588:592	water	588:592	water	588:592	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	7	32	theme	water	1027:1031	arg1	resistance					1033:1042	water resistance	1027:1042	water resistance	1027:1042	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	7	33	theme	functional	1134:1143	arg1	applications					1169:1180	functional antimicrobial packaging applications	1134:1180	functional antimicrobial packaging applications	1134:1180	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	0	34	theme	papers	40:45	arg1	Development					0:10	Development	0:10	Development of functional antimicrobial papers	0:45	Development of functional antimicrobial papers using chitosan/starch-silver nanoparticles.					
29391228	3	35	theme	9:1	432:434	arg1	ratios					422:427	different ratios	412:427	different ratios of 9:1, 8:2, 7:3, and 5:5 by weight	412:463	Chitosan was mixed into the synthesized St-AgNPs solution with different ratios of 9:1, 8:2, 7:3, and 5:5 by weight.					
29391228	3	36	theme	synthesized	377:387	arg1	solution					398:405	the synthesized St-AgNPs solution	373:405	the synthesized St-AgNPs solution	373:405	Chitosan was mixed into the synthesized St-AgNPs solution with different ratios of 9:1, 8:2, 7:3, and 5:5 by weight.					
29391228	1	37	theme	antimicrobial	208:220	arg1	applications					232:243	antimicrobial packaging applications	208:243	antimicrobial packaging applications	208:243	In the present work, we report the synthesis of chitosan:starch‑silver nanoparticle (Cht:St-AgNPs) coated papers for antimicrobial packaging applications.					
29391228	3	38	theme	different	412:420	arg1	ratios					422:427	different ratios	412:427	different ratios of 9:1, 8:2, 7:3, and 5:5 by weight	412:463	Chitosan was mixed into the synthesized St-AgNPs solution with different ratios of 9:1, 8:2, 7:3, and 5:5 by weight.					
29391228	4	39	theme	ratios	502:507	arg1	influence					479:487	the influence	475:487	the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities	475:641	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	5	40	theme	Cht	764:766	arg1	composition					749:759	the composition	745:759	the composition of Cht:St-AgNPs	745:775	It was observed that the properties of the coated papers were strongly dependent on the composition of Cht:St-AgNPs.					
29391228	1	41	dep	Cht	176:178	arg1	St-AgNPs					180:187	St-AgNPs	180:187	Cht:St-AgNPs	176:187	In the present work, we report the synthesis of chitosan:starch‑silver nanoparticle (Cht:St-AgNPs) coated papers for antimicrobial packaging applications.					
29391228	7	42	theme	remarkable	972:981	arg1	enhancement					983:993	a remarkable enhancement	970:993	a remarkable enhancement	970:993	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	0	43	theme	chitosan/starch-silver	53:74	arg1	nanoparticles					76:88	chitosan/starch-silver nanoparticles	53:88	chitosan/starch-silver nanoparticles	53:88	Development of functional antimicrobial papers using chitosan/starch-silver nanoparticles.					
29391228	4	44	theme	Cht	512:514	arg1	ratios					502:507	different ratios	492:507	different ratios of Cht:St-AgNPs	492:523	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	6	45	theme	St-AgNPs-coated	786:800	arg1	paper					802:806	St-AgNPs-coated paper	786:806	The Cht:St-AgNPs-coated paper prepared with the ratio of 9:1	778:837	The Cht:St-AgNPs-coated paper prepared with the ratio of 9:1 showed excellent mechanical properties and good resistance properties against water and oil.					
29391228	2	46	theme	7 nm	343:346	arg1	size					335:338	an average particle size	315:338	an average particle size of 7 nm	315:346	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	2	47	from	spherical	291:299	arg1	shape					304:308	shape	304:308	shape	304:308	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	1	48	theme	packaging	222:230	arg1	applications					232:243	antimicrobial packaging applications	208:243	antimicrobial packaging applications	208:243	In the present work, we report the synthesis of chitosan:starch‑silver nanoparticle (Cht:St-AgNPs) coated papers for antimicrobial packaging applications.					
29391228	7	49	theme	St-AgNPs	940:947	arg1	papers					956:961	St-AgNPs coated papers	940:961	St-AgNPs coated papers	940:961	The Cht:St-AgNPs coated papers showed a remarkable enhancement in mechanical strength, oil and water resistance, and antibacterial and antifungal activity, which can make them a potential candidate for functional antimicrobial packaging applications.					
29391228	1	50	theme	chitosan	139:146	arg1	synthesis					126:134	the synthesis	122:134	the synthesis of chitosan	122:146	In the present work, we report the synthesis of chitosan:starch‑silver nanoparticle (Cht:St-AgNPs) coated papers for antimicrobial packaging applications.					
29391228	2	51	with	spherical	291:299	arg1	size					335:338	an average particle size	315:338	an average particle size of 7 nm	315:346	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	2	52	theme	synthesized	266:276	arg1	spherical					291:299	spherical	291:299	spherical	291:299	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	2	52	theme	synthesized	266:276	arg1	St-AgNPs					278:285	The starch-assisted synthesized St-AgNPs	246:285	The starch-assisted synthesized St-AgNPs	246:285	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	4	53	theme	oil	598:600	arg1	resistance					602:611	oil resistance	598:611	oil resistance	598:611	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	2	54	from	shape	304:308	arg1	spherical					291:299	spherical	291:299	spherical	291:299	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	2	54	from	shape	304:308	arg1	St-AgNPs					278:285	The starch-assisted synthesized St-AgNPs	246:285	The starch-assisted synthesized St-AgNPs	246:285	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	6	55	theme	good	882:885	arg1	properties					898:907	good resistance properties	882:907	good resistance properties	882:907	The Cht:St-AgNPs-coated paper prepared with the ratio of 9:1 showed excellent mechanical properties and good resistance properties against water and oil.					
29391228	2	56	theme	starch-assisted	250:264	arg1	spherical					291:299	spherical	291:299	spherical	291:299	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	2	56	theme	starch-assisted	250:264	arg1	St-AgNPs					278:285	The starch-assisted synthesized St-AgNPs	246:285	The starch-assisted synthesized St-AgNPs	246:285	The starch-assisted synthesized St-AgNPs are spherical in shape with an average particle size of 7 nm.					
29391228	4	57	theme	mechanical	565:574	arg1	properties					576:585	mechanical properties	565:585	mechanical properties	565:585	Further, the influence of different ratios of Cht:St-AgNPs on the various paper properties such as mechanical properties, water and oil resistance, and antimicrobial activities was investigated.					
29391228	1	58	theme	starch‑silver	148:160	arg1	nanoparticle					162:173	starch‑silver nanoparticle	148:173	starch‑silver nanoparticle (Cht:St-AgNPs)	148:188	In the present work, we report the synthesis of chitosan:starch‑silver nanoparticle (Cht:St-AgNPs) coated papers for antimicrobial packaging applications.					
30732819	0	0	theme	Euryale	80:86	arg1	Salisb					94:99	Euryale ferox Salisb	80:99	Euryale ferox Salisb	80:99	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	9	1	theme	diabetes	998:1005	arg1	therapy					1007:1013	diabetes therapy	998:1013	diabetes therapy	998:1013	Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.					
30732819	0	2	from	characterization	11:26	arg1	Salisb					94:99	Euryale ferox Salisb	80:99	Euryale ferox Salisb	80:99	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	7	3	theme	EFSP-1	667:672	arg1	effect					657:662	the hypoglycemic effect	640:662	the hypoglycemic effect of EFSP-1	640:672	Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells.					
30732819	6	4	theme	1→4	551:553	arg1	-α-D-Glcp					555:563	(1→4)-α-D-Glcp	550:563	(1→4)-α-D-Glcp with branches substituted at O-6	550:596	The structure of EFSP-1 was characterized by analysis of FT-IR, GC-MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp.					
30732819	7	5	theme	hypoglycemic	644:655	arg1	effect					657:662	the hypoglycemic effect	640:662	the hypoglycemic effect of EFSP-1	640:672	Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells.					
30732819	0	6	theme	ferox	88:92	arg1	Salisb					94:99	Euryale ferox Salisb	80:99	Euryale ferox Salisb	80:99	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	5	7	theme	Monosaccharides	331:345	arg1	analysis					359:366	Monosaccharides composition analysis	331:366	Monosaccharides composition analysis	331:366	Monosaccharides composition analysis indicated that EFSP-1 was a glucan.					
30732819	2	8	theme	polysaccharide	129:142	arg1	EFSP-1					154:159	EFSP-1	154:159	EFSP-1	154:159	In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb.					
30732819	2	8	theme	polysaccharide	129:142	arg1	fraction					144:151	a polysaccharide fraction	127:151	a polysaccharide fraction (EFSP-1)	127:160	In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb.					
30732819	3	9	theme	DEAE	218:221	arg1	FF					233:234	DEAE sepharose FF and Superdex™ 75 gel chromatography	218:270	FF	233:234	by DEAE sepharose FF and Superdex™ 75 gel chromatography.					
30732819	7	10	dep	HepG2	726:730	arg1	cells					743:747	cells	743:747	cells	743:747	Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells.					
30732819	3	11	theme	sepharose	223:231	arg1	FF					233:234	DEAE sepharose FF and Superdex™ 75 gel chromatography	218:270	FF	233:234	by DEAE sepharose FF and Superdex™ 75 gel chromatography.					
30732819	8	12	from	pathway	889:895	arg1	cells					903:907	IR cells	900:907	IR cells	900:907	The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells.					
30732819	0	13	from	activity	54:61	arg1	Salisb					94:99	Euryale ferox Salisb	80:99	Euryale ferox Salisb	80:99	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	0	14	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	6	15	with	-α-D-Glcp	555:563	arg1	branches					570:577	branches	570:577	branches substituted at O-6	570:596	The structure of EFSP-1 was characterized by analysis of FT-IR, GC-MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp.					
30732819	8	16	theme	GLUT-4	851:856	arg1	expression					837:846	the expression	833:846	the expression of GLUT-4	833:856	The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells.					
30732819	0	17	theme	in	32:33	arg1	activity					54:61	in vitro hypoglycemic activity	32:61	in vitro hypoglycemic activity	32:61	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	3	18	theme	Superdex™	240:248	arg1	chromatography					257:270	DEAE sepharose FF and Superdex™ 75 gel chromatography	218:270	chromatography	257:270	by DEAE sepharose FF and Superdex™ 75 gel chromatography.					
30732819	8	19	theme	IR	900:901	arg1	cells					903:907	IR cells	900:907	IR cells	900:907	The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells.					
30732819	6	20	theme	FT-IR	461:465	arg1	analysis					449:456	analysis	449:456	analysis of FT-IR, GC-MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp	449:627	The structure of EFSP-1 was characterized by analysis of FT-IR, GC-MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp.					
30732819	0	21	theme	hypoglycemic	41:52	arg1	activity					54:61	in vitro hypoglycemic activity	32:61	in vitro hypoglycemic activity	32:61	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	2	22	theme	Salisb	207:212	arg1	seeds					184:188	the seeds	180:188	the seeds of Euryale ferox Salisb	180:212	In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb.					
30732819	6	23	theme	EFSP-1	520:525	arg1	backbone					508:515	the backbone	504:515	the backbone of EFSP-1	504:525	The structure of EFSP-1 was characterized by analysis of FT-IR, GC-MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp.					
30732819	9	24	theme	potential	935:943	arg1	EFSP-1					917:922	EFSP-1	917:922	EFSP-1	917:922	Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.					
30732819	9	24	theme	potential	935:943	arg1	food					956:959	a potential functional food	933:959	a potential functional food to ameliorate insulin resistance for diabetes therapy	933:1013	Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.					
30732819	2	25	theme	ferox	201:205	arg1	Salisb					207:212	Euryale ferox Salisb	193:212	Euryale ferox Salisb	193:212	In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb.					
30732819	8	26	theme	signal	882:887	arg1	pathway					889:895	PI3K/Akt signal pathway	873:895	PI3K/Akt signal pathway in IR cells	873:907	The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells.					
30732819	9	27	theme	functional	945:954	arg1	EFSP-1					917:922	EFSP-1	917:922	EFSP-1	917:922	Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.					
30732819	9	27	theme	functional	945:954	arg1	food					956:959	a potential functional food	933:959	a potential functional food to ameliorate insulin resistance for diabetes therapy	933:1013	Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.					
30732819	2	28	theme	Euryale	193:199	arg1	Salisb					207:212	Euryale ferox Salisb	193:212	Euryale ferox Salisb	193:212	In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb.					
30732819	8	29	theme	PI3K/Akt	873:880	arg1	pathway					889:895	PI3K/Akt signal pathway	873:895	PI3K/Akt signal pathway in IR cells	873:907	The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells.					
30732819	7	30	theme	insulin	707:713	arg1	resistance					715:724	insulin resistance HepG2 and 3T3-L1 cells	707:747	insulin resistance HepG2 and 3T3-L1 cells	707:747	Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells.					
30732819	4	31	theme	EFSP-1	310:315	arg1	weight					295:300	The average molecular weight	273:300	The average molecular weight (Mw) of EFSP-1	273:315	The average molecular weight (Mw) of EFSP-1 was 8.75 kDa.					
30732819	4	31	theme	EFSP-1	310:315	arg1	8.75 kDa					321:328	8.75 kDa	321:328	8.75 kDa	321:328	The average molecular weight (Mw) of EFSP-1 was 8.75 kDa.					
30732819	4	31	theme	EFSP-1	310:315	arg1	Mw					303:304	Mw	303:304	Mw	303:304	The average molecular weight (Mw) of EFSP-1 was 8.75 kDa.					
30732819	3	32	theme	gel	253:255	arg1	chromatography					257:270	DEAE sepharose FF and Superdex™ 75 gel chromatography	218:270	chromatography	257:270	by DEAE sepharose FF and Superdex™ 75 gel chromatography.					
30732819	0	33	dep	in	32:33	arg1	vitro					35:39	vitro	35:39	vitro	35:39	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	7	34	dep	resistance	715:724	arg1	HepG2					726:730	HepG2	726:730	HepG2	726:730	Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells.					
30732819	7	34	dep	resistance	715:724	arg1	3T3-L1					736:741	3T3-L1	736:741	3T3-L1	736:741	Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells.					
30732819	5	35	theme	composition	347:357	arg1	analysis					359:366	Monosaccharides composition analysis	331:366	Monosaccharides composition analysis	331:366	Monosaccharides composition analysis indicated that EFSP-1 was a glucan.					
30732819	6	36	theme	EFSP-1	421:426	arg1	structure					408:416	The structure	404:416	The structure of EFSP-1	404:426	The structure of EFSP-1 was characterized by analysis of FT-IR, GC-MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp.					
30732819	0	37	from	Salisb	94:99	arg1	activity					54:61	in vitro hypoglycemic activity	32:61	in vitro hypoglycemic activity	32:61	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	0	37	from	Salisb	94:99	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	0	37	from	Salisb	94:99	arg1	glucan					68:73	a glucan	66:73	a glucan from Euryale ferox Salisb	66:99	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	4	38	theme	molecular	285:293	arg1	weight					295:300	The average molecular weight	273:300	The average molecular weight (Mw) of EFSP-1	273:315	The average molecular weight (Mw) of EFSP-1 was 8.75 kDa.					
30732819	4	38	theme	molecular	285:293	arg1	8.75 kDa					321:328	8.75 kDa	321:328	8.75 kDa	321:328	The average molecular weight (Mw) of EFSP-1 was 8.75 kDa.					
30732819	4	38	theme	molecular	285:293	arg1	Mw					303:304	Mw	303:304	Mw	303:304	The average molecular weight (Mw) of EFSP-1 was 8.75 kDa.					
30732819	9	39	theme	insulin	975:981	arg1	resistance					983:992	insulin resistance	975:992	insulin resistance for diabetes therapy	975:1013	Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.					
30732819	0	40	theme	glucan	68:73	arg1	activity					54:61	in vitro hypoglycemic activity	32:61	in vitro hypoglycemic activity	32:61	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	0	40	theme	glucan	68:73	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and in vitro hypoglycemic activity of a glucan from Euryale ferox Salisb.					
30732819	8	41	theme	glucose	796:802	arg1	consumption					804:814	glucose consumption	796:814	glucose consumption	796:814	The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells.					
30732819	4	42	theme	average	277:283	arg1	weight					295:300	The average molecular weight	273:300	The average molecular weight (Mw) of EFSP-1	273:315	The average molecular weight (Mw) of EFSP-1 was 8.75 kDa.					
30732819	4	42	theme	average	277:283	arg1	8.75 kDa					321:328	8.75 kDa	321:328	8.75 kDa	321:328	The average molecular weight (Mw) of EFSP-1 was 8.75 kDa.					
30732819	4	42	theme	average	277:283	arg1	Mw					303:304	Mw	303:304	Mw	303:304	The average molecular weight (Mw) of EFSP-1 was 8.75 kDa.					
31760030	7	0	theme	mechanical	1375:1384	arg1	integrity					1386:1394	excellent mechanical integrity	1365:1394	excellent mechanical integrity	1365:1394	The excellent mechanical integrity and reusable adsorption performance of GO/CS/PDA promise the adsorbent with satisfactory reusability.					
31760030	4	1	theme	introduced	652:661	arg1	PDA					663:665	the introduced PDA	648:665	the introduced PDA	648:665	GO was cross-linked with CS, forming a reinforced and three-dimensional macroporous structure; the introduced PDA was simultaneously cross-linked with CS and GO, providing more abundant nanopores and active sites for Cr(VI) removal.					
31760030	0	2	dep	removal	123:129	arg1	VI					119:120	VI	119:120	VI	119:120	Biomaterials cross-linked graphene oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal.					
31760030	5	3	with	dependent	984:992	arg1	rate					1020:1023	the highest removal rate	1000:1023	the highest removal rate at pH = 3.0	1000:1035	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	1	4	theme	abundant	245:252	arg1	sites					265:269	abundant adsorption sites	245:269	abundant adsorption sites	245:269	Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites.					
31760030	5	5	theme	optimized	848:856	arg1	1:20:2					870:875	1:20:2	870:875	1:20:2	870:875	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	5	theme	optimized	848:856	arg1	ratio					863:867	an optimized mass ratio	845:867	an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption	845:936	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	7	6	dep	integrity	1386:1394	arg1	The					1361:1363	The	1361:1363	The	1361:1363	The excellent mechanical integrity and reusable adsorption performance of GO/CS/PDA promise the adsorbent with satisfactory reusability.					
31760030	1	7	theme	adsorption	254:263	arg1	sites					265:269	abundant adsorption sites	245:269	abundant adsorption sites	245:269	Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites.					
31760030	0	8	theme	Cr	115:116	arg1	removal					123:129	efficient Cr (VI) removal	105:129	efficient Cr (VI) removal	105:129	Biomaterials cross-linked graphene oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal.					
31760030	5	9	theme	PDA	893:895	arg1	1:20:2					870:875	1:20:2	870:875	1:20:2	870:875	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	9	theme	PDA	893:895	arg1	ratio					863:867	an optimized mass ratio	845:867	an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption	845:936	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	6	10	theme	adsorption	1127:1136	arg1	kinetics					1138:1145	the adsorption kinetics	1123:1145	the adsorption kinetics	1123:1145	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	2	11	from	dissolubility	323:335	arg1	conditions					347:356	acidic conditions	340:356	acidic conditions of CS	340:362	However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application.					
31760030	2	12	theme	weak	285:288	arg1	properties					301:310	weak mechanical properties	285:310	weak mechanical properties	285:310	However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application.					
31760030	5	13	dep	adsorption	927:936	arg1	VI					923:924	VI	923:924	VI	923:924	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	0	14	theme	efficient	105:113	arg1	removal					123:129	efficient Cr (VI) removal	105:129	efficient Cr (VI) removal	105:129	Biomaterials cross-linked graphene oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal.					
31760030	3	15	theme	composite	514:522	arg1	aerogel					524:530	CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel	466:530	CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel	466:530	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	2	16	theme	CS	361:362	arg1	conditions					347:356	acidic conditions	340:356	acidic conditions of CS	340:362	However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application.					
31760030	3	17	theme	cross-linked	469:480	arg1	GO/CS/PDA					503:511	GO/CS/PDA	503:511	GO/CS/PDA	503:511	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	3	17	theme	cross-linked	469:480	arg1	oxide					491:495	cross-linked graphene oxide	469:495	CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel	466:530	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	6	18	theme	exothermic	1341:1350	arg1	adsorption					1308:1317	the adsorption	1304:1317	the adsorption	1304:1317	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	6	18	theme	exothermic	1341:1350	arg1	process					1352:1358	a spontaneous and exothermic process	1323:1358	a spontaneous and exothermic process	1323:1358	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	4	19	theme	abundant	730:737	arg1	nanopores					739:747	more abundant nanopores	725:747	more abundant nanopores	725:747	GO was cross-linked with CS, forming a reinforced and three-dimensional macroporous structure; the introduced PDA was simultaneously cross-linked with CS and GO, providing more abundant nanopores and active sites for Cr(VI) removal.					
31760030	4	20	theme	macroporous	625:635	arg1	structure					637:645	a reinforced and three-dimensional macroporous structure	590:645	a reinforced and three-dimensional macroporous structure	590:645	GO was cross-linked with CS, forming a reinforced and three-dimensional macroporous structure; the introduced PDA was simultaneously cross-linked with CS and GO, providing more abundant nanopores and active sites for Cr(VI) removal.					
31760030	6	21	theme	pseudo-second-order	1042:1060	arg1	models					1085:1090	The pseudo-second-order kinetic and Freundlich models	1038:1090	The pseudo-second-order kinetic and Freundlich models	1038:1090	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	6	21	theme	pseudo-second-order	1042:1060	arg1	suitable					1102:1109	suitable	1102:1109	suitable	1102:1109	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	6	22	theme	adsorption	1203:1212	arg1	312.05 mg/g					1241:1251	312.05 mg/g	1241:1251	312.05 mg/g	1241:1251	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	6	22	theme	adsorption	1203:1212	arg1	capacity					1214:1221	the maximum adsorption capacity	1191:1221	the maximum adsorption capacity for GO/CS/PDA	1191:1235	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	3	23	theme	facile	403:408	arg1	method					410:415	a facile method	401:415	a facile method	401:415	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	1	24	theme	attractive	152:161	arg1	bio-adsorbent					163:175	an attractive bio-adsorbent	149:175	an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites	149:269	Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites.					
31760030	1	24	theme	attractive	152:161	arg1	Chitosan					132:139	Chitosan	132:139	Chitosan (CS)	132:144	Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites.					
31760030	6	25	theme	298 K.	1256:1261	arg1	parameters					1278:1287	298 K. Thermodynamics parameters	1256:1287	298 K. Thermodynamics parameters	1256:1287	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	2	26	theme	strong	316:321	arg1	dissolubility					323:335	strong dissolubility	316:335	strong dissolubility	316:335	However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application.					
31760030	5	27	theme	Cr	970:971	arg1	dependent					984:992	dependent	984:992	dependent	984:992	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	27	theme	Cr	970:971	arg1	VI					973:974	VI	973:974	VI	973:974	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	27	theme	Cr	970:971	arg1	rate					962:965	the adsorption removal rate	939:965	the adsorption removal rate of Cr	939:971	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	3	28	dep	removal	544:550	arg1	VI					540:541	VI	540:541	VI	540:541	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	5	29	theme	removal	1012:1018	arg1	rate					1020:1023	the highest removal rate	1000:1023	the highest removal rate at pH = 3.0	1000:1035	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	2	30	theme	wide	375:378	arg1	application					380:390	its wide application	371:390	its wide application	371:390	However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application.					
31760030	0	31	theme	oxide	35:39	arg1	aerogel					51:57	oxide composite aerogel	35:57	oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal	35:129	Biomaterials cross-linked graphene oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal.					
31760030	5	32	dep	dependent	984:992	arg1	dependent					984:992	dependent	984:992	dependent	984:992	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	32	dep	dependent	984:992	arg1	VI					973:974	VI	973:974	VI	973:974	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	32	dep	dependent	984:992	arg1	rate					962:965	the adsorption removal rate	939:965	the adsorption removal rate of Cr	939:971	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	6	33	theme	maximum	1195:1201	arg1	312.05 mg/g					1241:1251	312.05 mg/g	1241:1251	312.05 mg/g	1241:1251	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	6	33	theme	maximum	1195:1201	arg1	capacity					1214:1221	the maximum adsorption capacity	1191:1221	the maximum adsorption capacity for GO/CS/PDA	1191:1235	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	1	34	theme	pollutant	180:188	arg1	removal					190:196	pollutant removal	180:196	pollutant removal due to its environment-friendly properties and abundant adsorption sites	180:269	Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites.					
31760030	7	35	theme	satisfactory	1472:1483	arg1	reusability					1485:1495	satisfactory reusability	1472:1495	satisfactory reusability	1472:1495	The excellent mechanical integrity and reusable adsorption performance of GO/CS/PDA promise the adsorbent with satisfactory reusability.					
31760030	5	36	theme	CS	885:886	arg1	1:20:2					870:875	1:20:2	870:875	1:20:2	870:875	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	36	theme	CS	885:886	arg1	ratio					863:867	an optimized mass ratio	845:867	an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption	845:936	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	7	37	theme	GO/CS/PDA	1435:1443	arg1	integrity					1386:1394	excellent mechanical integrity	1365:1394	excellent mechanical integrity	1365:1394	The excellent mechanical integrity and reusable adsorption performance of GO/CS/PDA promise the adsorbent with satisfactory reusability.					
31760030	7	37	theme	GO/CS/PDA	1435:1443	arg1	performance					1420:1430	reusable adsorption performance	1400:1430	reusable adsorption performance	1400:1430	The excellent mechanical integrity and reusable adsorption performance of GO/CS/PDA promise the adsorbent with satisfactory reusability.					
31760030	2	38	theme	mechanical	290:299	arg1	properties					301:310	weak mechanical properties	285:310	weak mechanical properties	285:310	However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application.					
31760030	3	39	theme	oxide	491:495	arg1	aerogel					524:530	CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel	466:530	CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel	466:530	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	3	40	theme	graphene	482:489	arg1	GO/CS/PDA					503:511	GO/CS/PDA	503:511	GO/CS/PDA	503:511	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	3	40	theme	graphene	482:489	arg1	oxide					491:495	cross-linked graphene oxide	469:495	CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel	466:530	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	6	41	theme	Freundlich	1074:1083	arg1	models					1085:1090	The pseudo-second-order kinetic and Freundlich models	1038:1090	The pseudo-second-order kinetic and Freundlich models	1038:1090	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	6	41	theme	Freundlich	1074:1083	arg1	suitable					1102:1109	suitable	1102:1109	suitable	1102:1109	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	5	42	theme	adsorption	943:952	arg1	dependent					984:992	dependent	984:992	dependent	984:992	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	42	theme	adsorption	943:952	arg1	VI					973:974	VI	973:974	VI	973:974	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	42	theme	adsorption	943:952	arg1	rate					962:965	the adsorption removal rate	939:965	the adsorption removal rate of Cr	939:971	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	4	43	theme	Cr	770:771	arg1	removal					777:783	Cr(VI) removal	770:783	Cr(VI) removal	770:783	GO was cross-linked with CS, forming a reinforced and three-dimensional macroporous structure; the introduced PDA was simultaneously cross-linked with CS and GO, providing more abundant nanopores and active sites for Cr(VI) removal.					
31760030	4	44	theme	three-dimensional	607:623	arg1	structure					637:645	a reinforced and three-dimensional macroporous structure	590:645	a reinforced and three-dimensional macroporous structure	590:645	GO was cross-linked with CS, forming a reinforced and three-dimensional macroporous structure; the introduced PDA was simultaneously cross-linked with CS and GO, providing more abundant nanopores and active sites for Cr(VI) removal.					
31760030	1	45	theme	due	198:200	arg1	removal					190:196	pollutant removal	180:196	pollutant removal due to its environment-friendly properties and abundant adsorption sites	180:269	Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites.					
31760030	0	46	theme	composite	41:49	arg1	aerogel					51:57	oxide composite aerogel	35:57	oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal	35:129	Biomaterials cross-linked graphene oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal.					
31760030	3	47	dep	oxide	491:495	arg1	GO					498:499	GO	498:499	GO	498:499	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	5	48	theme	batch	799:803	arg1	results					816:822	the batch experiment results	795:822	the batch experiment results	795:822	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	49	theme	removal	954:960	arg1	dependent					984:992	dependent	984:992	dependent	984:992	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	49	theme	removal	954:960	arg1	VI					973:974	VI	973:974	VI	973:974	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	49	theme	removal	954:960	arg1	rate					962:965	the adsorption removal rate	939:965	the adsorption removal rate of Cr	939:971	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	4	50	dep	removal	777:783	arg1	VI					773:774	VI	773:774	VI	773:774	GO was cross-linked with CS, forming a reinforced and three-dimensional macroporous structure; the introduced PDA was simultaneously cross-linked with CS and GO, providing more abundant nanopores and active sites for Cr(VI) removal.					
31760030	5	51	theme	highest	1004:1010	arg1	rate					1020:1023	the highest removal rate	1000:1023	the highest removal rate at pH = 3.0	1000:1035	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	52	theme	Cr	920:921	arg1	adsorption					927:936	the most effective Cr(VI) adsorption	901:936	the most effective Cr(VI) adsorption	901:936	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	2	53	dep	properties	301:310	arg1	the					281:283	the	281:283	the	281:283	However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application.					
31760030	6	54	theme	Thermodynamics	1263:1276	arg1	parameters					1278:1287	298 K. Thermodynamics parameters	1256:1287	298 K. Thermodynamics parameters	1256:1287	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	7	55	theme	reusable	1400:1407	arg1	performance					1420:1430	reusable adsorption performance	1400:1430	reusable adsorption performance	1400:1430	The excellent mechanical integrity and reusable adsorption performance of GO/CS/PDA promise the adsorbent with satisfactory reusability.					
31760030	5	56	from	pH = 3.0	1028:1035	arg1	rate					1020:1023	the highest removal rate	1000:1023	the highest removal rate at pH = 3.0	1000:1035	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	2	57	theme	acidic	340:345	arg1	conditions					347:356	acidic conditions	340:356	acidic conditions of CS	340:362	However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application.					
31760030	3	58	theme	Cr	536:537	arg1	removal					544:550	Cr (VI) removal	536:550	Cr (VI) removal	536:550	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	3	59	link	cross-linked	469:480	arg1	GO/CS/PDA					503:511	GO/CS/PDA	503:511	GO/CS/PDA	503:511	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	3	59	link	cross-linked	469:480	arg1	oxide					491:495	cross-linked graphene oxide	469:495	CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel	466:530	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	7	60	theme	adsorption	1409:1418	arg1	performance					1420:1430	reusable adsorption performance	1400:1430	reusable adsorption performance	1400:1430	The excellent mechanical integrity and reusable adsorption performance of GO/CS/PDA promise the adsorbent with satisfactory reusability.					
31760030	6	61	theme	kinetic	1062:1068	arg1	models					1085:1090	The pseudo-second-order kinetic and Freundlich models	1038:1090	The pseudo-second-order kinetic and Freundlich models	1038:1090	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	6	61	theme	kinetic	1062:1068	arg1	suitable					1102:1109	suitable	1102:1109	suitable	1102:1109	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	5	62	theme	effective	910:918	arg1	adsorption					927:936	the most effective Cr(VI) adsorption	901:936	the most effective Cr(VI) adsorption	901:936	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	63	theme	mass	858:861	arg1	1:20:2					870:875	1:20:2	870:875	1:20:2	870:875	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	63	theme	mass	858:861	arg1	ratio					863:867	an optimized mass ratio	845:867	an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption	845:936	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	64	theme	experiment	805:814	arg1	results					816:822	the batch experiment results	795:822	the batch experiment results	795:822	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	6	65	theme	adsorption	1151:1160	arg1	isotherms					1162:1170	adsorption isotherms	1151:1170	adsorption isotherms	1151:1170	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	5	66	theme	GO	881:882	arg1	1:20:2					870:875	1:20:2	870:875	1:20:2	870:875	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	5	66	theme	GO	881:882	arg1	ratio					863:867	an optimized mass ratio	845:867	an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption	845:936	Based on the batch experiment results, GO/CS/PDA exhibited an optimized mass ratio (1:20:2) of GO, CS, and PDA for the most effective Cr(VI) adsorption; the adsorption removal rate of Cr(VI) was pH dependent, with the highest removal rate at pH = 3.0.					
31760030	1	67	from	bio-adsorbent	163:175	arg1	removal					190:196	pollutant removal	180:196	pollutant removal due to its environment-friendly properties and abundant adsorption sites	180:269	Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites.					
31760030	3	68	theme	CS	466:467	arg1	aerogel					524:530	CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel	466:530	CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel	466:530	Herein, a facile method was proposed to fabricate polydopamine (PDA) and CS cross-linked graphene oxide (GO) (GO/CS/PDA) composite aerogel for Cr (VI) removal.					
31760030	1	69	theme	environment-friendly	209:228	arg1	properties					230:239	its environment-friendly properties	205:239	its environment-friendly properties	205:239	Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites.					
31760030	0	70	theme	network	83:89	arg1	structure					91:99	a macro-nanoporous network structure	64:99	a macro-nanoporous network structure for efficient Cr (VI) removal	64:129	Biomaterials cross-linked graphene oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal.					
31760030	6	71	theme	spontaneous	1325:1335	arg1	adsorption					1308:1317	the adsorption	1304:1317	the adsorption	1304:1317	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	6	71	theme	spontaneous	1325:1335	arg1	process					1352:1358	a spontaneous and exothermic process	1323:1358	a spontaneous and exothermic process	1323:1358	The pseudo-second-order kinetic and Freundlich models were more suitable for fitting the adsorption kinetics and adsorption isotherms, respectively, and the maximum adsorption capacity for GO/CS/PDA was 312.05 mg/g at 298 K. Thermodynamics parameters indicated that the adsorption was a spontaneous and exothermic process.					
31760030	4	72	theme	reinforced	592:601	arg1	structure					637:645	a reinforced and three-dimensional macroporous structure	590:645	a reinforced and three-dimensional macroporous structure	590:645	GO was cross-linked with CS, forming a reinforced and three-dimensional macroporous structure; the introduced PDA was simultaneously cross-linked with CS and GO, providing more abundant nanopores and active sites for Cr(VI) removal.					
31760030	0	73	theme	macro-nanoporous	66:81	arg1	structure					91:99	a macro-nanoporous network structure	64:99	a macro-nanoporous network structure for efficient Cr (VI) removal	64:129	Biomaterials cross-linked graphene oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal.					
31760030	7	74	theme	excellent	1365:1373	arg1	integrity					1386:1394	excellent mechanical integrity	1365:1394	excellent mechanical integrity	1365:1394	The excellent mechanical integrity and reusable adsorption performance of GO/CS/PDA promise the adsorbent with satisfactory reusability.					
31760030	4	75	theme	active	753:758	arg1	sites					760:764	active sites	753:764	active sites	753:764	GO was cross-linked with CS, forming a reinforced and three-dimensional macroporous structure; the introduced PDA was simultaneously cross-linked with CS and GO, providing more abundant nanopores and active sites for Cr(VI) removal.					
31760030	0	76	with	aerogel	51:57	arg1	structure					91:99	a macro-nanoporous network structure	64:99	a macro-nanoporous network structure for efficient Cr (VI) removal	64:129	Biomaterials cross-linked graphene oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal.					
31760030	2	77	from	properties	301:310	arg1	conditions					347:356	acidic conditions	340:356	acidic conditions of CS	340:362	However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application.					
31678524	8	0	theme	large	1119:1123	arg1	number					1125:1130	a large number	1117:1130	a large number of samples	1117:1141	The proposed models lay the foundation of a platform for faster assessment of a large number of samples and without the use of animals.					
31678524	4	1	theme	main	548:551	arg1	barriers					553:560	the main barriers	544:560	the main barriers encountered upon intravitreal injection	544:600	This work aimed to establish a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection.					
31678524	4	1	theme	main	548:551	arg1	vitreous					534:541	the vitreous	530:541	the vitreous	530:541	This work aimed to establish a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection.					
31678524	0	2	theme	barrier	71:77	arg1	model					44:48	a multilayered 3D cellular model	17:48	a multilayered 3D cellular model of the retinal-blood barrier	17:77	Establishment of a multilayered 3D cellular model of the retinal-blood barrier.					
31678524	4	3	dep	BRB	504:506	arg1	the					500:502	the	500:502	the	500:502	This work aimed to establish a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection.					
31678524	5	4	theme	co-culture	644:653	arg1	time					655:658	co-culture time	644:658	co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous	644:767	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	6	5	theme	similar	827:833	arg1	properties					816:825	viscoelastic properties	803:825	viscoelastic properties similar to human vitreous	803:851	The developed vitreous mimic has viscoelastic properties similar to human vitreous.					
31678524	5	6	theme	hydrogels	724:732	arg1	HUVECs					675:680	HUVECs	675:680	HUVECs	675:680	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	5	6	theme	hydrogels	724:732	arg1	ARPE-19					663:669	ARPE-19	663:669	ARPE-19	663:669	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	5	6	theme	hydrogels	724:732	arg1	compositions					708:719	different biomaterial compositions	686:719	different biomaterial compositions	686:719	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	8	7	theme	proposed	1043:1050	arg1	models					1052:1057	The proposed models	1039:1057	The proposed models	1039:1057	The proposed models lay the foundation of a platform for faster assessment of a large number of samples and without the use of animals.					
31678524	8	8	theme	platform	1083:1090	arg1	foundation					1067:1076	the foundation	1063:1076	the foundation of a platform for faster assessment of a large number of samples and without the use of animals	1063:1172	The proposed models lay the foundation of a platform for faster assessment of a large number of samples and without the use of animals.					
31678524	7	9	theme	endothelial	886:896	arg1	cells					898:902	human retinal and endothelial cells	868:902	cells	898:902	Co-culture of human retinal and endothelial cells showed increased transepithelial resistance with longer co-culture times concomitant with reduced permeability to FITC-dextran 40 kDa.					
31678524	4	10	theme	cellular	444:451	arg1	model					453:457	a 3D cellular model	439:457	a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection	439:600	This work aimed to establish a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection.					
31678524	6	11	theme	viscoelastic	803:814	arg1	properties					816:825	viscoelastic properties	803:825	viscoelastic properties similar to human vitreous	803:851	The developed vitreous mimic has viscoelastic properties similar to human vitreous.					
31678524	6	12	dep	vitreous	784:791	arg1	mimic					793:797	mimic	793:797	mimic	793:797	The developed vitreous mimic has viscoelastic properties similar to human vitreous.					
31678524	2	13	theme	treatment	138:146	arg1	strategies					148:157	treatment strategies	138:157	treatment strategies	138:157	Still, treatment strategies are limited and the challenging anatomical barriers of the eye limit the evaluation and development of new therapeutics.					
31678524	5	14	theme	Several	603:609	arg1	parameters					611:620	Several parameters	603:620	Several parameters	603:620	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	7	15	theme	reduced	994:1000	arg1	permeability					1002:1013	reduced permeability	994:1013	reduced permeability to FITC-dextran 40 kDa	994:1036	Co-culture of human retinal and endothelial cells showed increased transepithelial resistance with longer co-culture times concomitant with reduced permeability to FITC-dextran 40 kDa.					
31678524	2	16	theme	therapeutics	266:277	arg1	development					247:257	development	247:257	development	247:257	Still, treatment strategies are limited and the challenging anatomical barriers of the eye limit the evaluation and development of new therapeutics.					
31678524	2	16	theme	therapeutics	266:277	arg1	evaluation					232:241	evaluation	232:241	evaluation	232:241	Still, treatment strategies are limited and the challenging anatomical barriers of the eye limit the evaluation and development of new therapeutics.					
31678524	7	17	theme	retinal	874:880	arg1	Co-culture					854:863	Co-culture	854:863	Co-culture of human retinal and endothelial cells	854:902	Co-culture of human retinal and endothelial cells showed increased transepithelial resistance with longer co-culture times concomitant with reduced permeability to FITC-dextran 40 kDa.					
31678524	2	18	theme	new	262:264	arg1	therapeutics					266:277	new therapeutics	262:277	new therapeutics	262:277	Still, treatment strategies are limited and the challenging anatomical barriers of the eye limit the evaluation and development of new therapeutics.					
31678524	2	19	dep	evaluation	232:241	arg1	the					228:230	the	228:230	the	228:230	Still, treatment strategies are limited and the challenging anatomical barriers of the eye limit the evaluation and development of new therapeutics.					
31678524	3	20	from	choroid	377:383	arg1	retina					361:366	the retina	357:366	the retina from the choroid	357:383	Among these layers of barriers is the blood-retinal barrier, which separates the retina from the choroid by the Bruch's membrane.					
31678524	0	21	theme	retinal-blood	57:69	arg1	barrier					71:77	the retinal-blood barrier	53:77	the retinal-blood barrier	53:77	Establishment of a multilayered 3D cellular model of the retinal-blood barrier.					
31678524	1	22	theme	Retinal	80:86	arg1	disorders					88:96	Retinal disorders	80:96	Retinal disorders	80:96	Retinal disorders are leading causes of blindness.					
31678524	1	22	theme	Retinal	80:86	arg1	causes					110:115	leading causes	102:115	leading causes of blindness	102:128	Retinal disorders are leading causes of blindness.					
31678524	4	23	theme	3D	441:442	arg1	model					453:457	a 3D cellular model	439:457	a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection	439:600	This work aimed to establish a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection.					
31678524	8	24	theme	samples	1135:1141	arg1	number					1125:1130	a large number	1117:1130	a large number of samples	1117:1141	The proposed models lay the foundation of a platform for faster assessment of a large number of samples and without the use of animals.					
31678524	6	25	theme	developed	774:782	arg1	vitreous					784:791	The developed vitreous	770:791	The developed vitreous mimic	770:797	The developed vitreous mimic has viscoelastic properties similar to human vitreous.					
31678524	8	26	theme	faster	1096:1101	arg1	assessment					1103:1112	faster assessment	1096:1112	faster assessment of a large number of samples and without the use of animals	1096:1172	The proposed models lay the foundation of a platform for faster assessment of a large number of samples and without the use of animals.					
31678524	0	27	theme	multilayered	19:30	arg1	model					44:48	a multilayered 3D cellular model	17:48	a multilayered 3D cellular model of the retinal-blood barrier	17:77	Establishment of a multilayered 3D cellular model of the retinal-blood barrier.					
31678524	7	28	theme	longer	953:958	arg1	times					971:975	longer co-culture times	953:975	longer co-culture times	953:975	Co-culture of human retinal and endothelial cells showed increased transepithelial resistance with longer co-culture times concomitant with reduced permeability to FITC-dextran 40 kDa.					
31678524	5	29	theme	different	686:694	arg1	compositions					708:719	different biomaterial compositions	686:719	different biomaterial compositions	686:719	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	7	30	theme	co-culture	960:969	arg1	times					971:975	longer co-culture times	953:975	longer co-culture times	953:975	Co-culture of human retinal and endothelial cells showed increased transepithelial resistance with longer co-culture times concomitant with reduced permeability to FITC-dextran 40 kDa.					
31678524	5	31	theme	biomaterial	696:706	arg1	compositions					708:719	different biomaterial compositions	686:719	different biomaterial compositions	686:719	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	1	32	theme	leading	102:108	arg1	disorders					88:96	Retinal disorders	80:96	Retinal disorders	80:96	Retinal disorders are leading causes of blindness.					
31678524	1	32	theme	leading	102:108	arg1	causes					110:115	leading causes	102:115	leading causes of blindness	102:128	Retinal disorders are leading causes of blindness.					
31678524	0	33	theme	cellular	35:42	arg1	model					44:48	a multilayered 3D cellular model	17:48	a multilayered 3D cellular model of the retinal-blood barrier	17:77	Establishment of a multilayered 3D cellular model of the retinal-blood barrier.					
31678524	7	34	with	concomitant	977:987	arg1	permeability					1002:1013	reduced permeability	994:1013	reduced permeability to FITC-dextran 40 kDa	994:1036	Co-culture of human retinal and endothelial cells showed increased transepithelial resistance with longer co-culture times concomitant with reduced permeability to FITC-dextran 40 kDa.					
31678524	0	35	theme	3D	32:33	arg1	model					44:48	a multilayered 3D cellular model	17:48	a multilayered 3D cellular model of the retinal-blood barrier	17:77	Establishment of a multilayered 3D cellular model of the retinal-blood barrier.					
31678524	4	36	theme	BRB	504:506	arg1	properties					486:495	barrier properties	478:495	barrier properties of the BRB and diffusion	478:520	This work aimed to establish a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection.					
31678524	8	37	theme	number	1125:1130	arg1	assessment					1103:1112	faster assessment	1096:1112	faster assessment of a large number of samples and without the use of animals	1096:1172	The proposed models lay the foundation of a platform for faster assessment of a large number of samples and without the use of animals.					
31678524	8	38	theme	animals	1166:1172	arg1	use					1159:1161	the use	1155:1161	the use of animals	1155:1172	The proposed models lay the foundation of a platform for faster assessment of a large number of samples and without the use of animals.					
31678524	5	39	theme	ARPE-19	663:669	arg1	time					655:658	co-culture time	644:658	co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous	644:767	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	2	40	theme	eye	218:220	arg1	barriers					202:209	the challenging anatomical barriers	175:209	the challenging anatomical barriers of the eye	175:220	Still, treatment strategies are limited and the challenging anatomical barriers of the eye limit the evaluation and development of new therapeutics.					
31678524	0	41	theme	model	44:48	arg1	Establishment					0:12	Establishment	0:12	Establishment of a multilayered 3D cellular model of the retinal-blood barrier.	0:78	Establishment of a multilayered 3D cellular model of the retinal-blood barrier.					
31678524	4	42	theme	diffusion	512:520	arg1	properties					486:495	barrier properties	478:495	barrier properties of the BRB and diffusion	478:520	This work aimed to establish a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection.					
31678524	3	43	theme	barriers	302:309	arg1	layers					292:297	these layers	286:297	these layers of barriers	286:309	Among these layers of barriers is the blood-retinal barrier, which separates the retina from the choroid by the Bruch's membrane.					
31678524	5	44	theme	HUVECs	675:680	arg1	time					655:658	co-culture time	644:658	co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous	644:767	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	6	45	contain	has	799:801	arg1	vitreous					784:791	The developed vitreous	770:791	The developed vitreous mimic	770:797	The developed vitreous mimic has viscoelastic properties similar to human vitreous.					
31678524	6	45	contain	has	799:801	arg2	properties					816:825	viscoelastic properties	803:825	viscoelastic properties similar to human vitreous	803:851	The developed vitreous mimic has viscoelastic properties similar to human vitreous.					
31678524	1	46	theme	blindness	120:128	arg1	disorders					88:96	Retinal disorders	80:96	Retinal disorders	80:96	Retinal disorders are leading causes of blindness.					
31678524	1	46	theme	blindness	120:128	arg1	causes					110:115	leading causes	102:115	leading causes of blindness	102:128	Retinal disorders are leading causes of blindness.					
31678524	4	47	theme	intravitreal	579:590	arg1	injection					592:600	intravitreal injection	579:600	intravitreal injection	579:600	This work aimed to establish a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection.					
31678524	2	48	theme	anatomical	191:200	arg1	barriers					202:209	the challenging anatomical barriers	175:209	the challenging anatomical barriers of the eye	175:220	Still, treatment strategies are limited and the challenging anatomical barriers of the eye limit the evaluation and development of new therapeutics.					
31678524	7	49	theme	increased	911:919	arg1	resistance					937:946	increased transepithelial resistance	911:946	increased transepithelial resistance	911:946	Co-culture of human retinal and endothelial cells showed increased transepithelial resistance with longer co-culture times concomitant with reduced permeability to FITC-dextran 40 kDa.					
31678524	3	50	theme	blood-retinal	318:330	arg1	barrier					332:338	the blood-retinal barrier	314:338	the blood-retinal barrier	314:338	Among these layers of barriers is the blood-retinal barrier, which separates the retina from the choroid by the Bruch's membrane.					
31678524	2	51	theme	challenging	179:189	arg1	barriers					202:209	the challenging anatomical barriers	175:209	the challenging anatomical barriers of the eye	175:220	Still, treatment strategies are limited and the challenging anatomical barriers of the eye limit the evaluation and development of new therapeutics.					
31678524	6	52	theme	human	838:842	arg1	vitreous					844:851	human vitreous	838:851	human vitreous	838:851	The developed vitreous mimic has viscoelastic properties similar to human vitreous.					
31678524	7	53	theme	transepithelial	921:935	arg1	resistance					937:946	increased transepithelial resistance	911:946	increased transepithelial resistance	911:946	Co-culture of human retinal and endothelial cells showed increased transepithelial resistance with longer co-culture times concomitant with reduced permeability to FITC-dextran 40 kDa.					
31678524	5	54	theme	human	754:758	arg1	vitreous					760:767	the human vitreous	750:767	the human vitreous	750:767	Several parameters were evaluated namely co-culture time of ARPE-19 and HUVECs and different biomaterial compositions of hydrogels to better mimic the human vitreous.					
31678524	4	55	theme	barrier	478:484	arg1	properties					486:495	barrier properties	478:495	barrier properties of the BRB and diffusion	478:520	This work aimed to establish a 3D cellular model that recapitulates barrier properties of the BRB and diffusion through the vitreous, the main barriers encountered upon intravitreal injection.					
31678524	7	56	theme	cells	898:902	arg1	Co-culture					854:863	Co-culture	854:863	Co-culture of human retinal and endothelial cells	854:902	Co-culture of human retinal and endothelial cells showed increased transepithelial resistance with longer co-culture times concomitant with reduced permeability to FITC-dextran 40 kDa.					
31328214	9	0	theme	digestion	1423:1431	arg1	kinetics					1433:1440	different starch digestion kinetics	1406:1440	different starch digestion kinetics	1406:1440	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	0	1	with	ways	76:79	arg1	fiber					103:107	fiber	103:107	fiber	103:107	Starch digestion kinetics of extruded reformed rice is changed in different ways with added protein or fiber.					
31328214	0	1	with	ways	76:79	arg1	protein					92:98	protein	92:98	protein	92:98	Starch digestion kinetics of extruded reformed rice is changed in different ways with added protein or fiber.					
31328214	2	2	theme	protein	289:295	arg1	SPI					306:308	SPI	306:308	SPI	306:308	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	2	theme	protein	289:295	arg1	isolate					297:303	added soy protein isolate	279:303	added soy protein isolate (SPI)	279:309	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	2	theme	protein	289:295	arg1	maltodextrin					356:367	resistant maltodextrin	346:367	resistant maltodextrin (RMD)]	346:374	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	2	theme	protein	289:295	arg1	CB					341:342	CB	341:342	CB	341:342	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	9	3	theme	extruded	1351:1358	arg1	compositions					1365:1376	extruded rice compositions	1351:1376	extruded rice compositions	1351:1376	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	6	4	theme	20	939:940	arg1	%					941:941	%	941:941	%	941:941	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	3	5	theme	%	610:610	arg1	rice					634:637	20% supplemented extruded rice	608:637	20% supplemented extruded rice	608:637	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	6	6	theme	single-phase	1000:1011	arg1	digestion					1021:1029	a single-phase kinetic digestion	998:1029	a single-phase kinetic digestion with similar k values	998:1051	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	4	7	theme	extruded	702:709	arg1	20SPI-ER					717:724	20% SPI extruded rice (20SPI-ER)	694:725	20% SPI extruded rice (20SPI-ER)	694:725	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	10	8	theme	half-emptying	1604:1616	arg1	time					1618:1621	lower gastric half-emptying time	1590:1621	lower gastric half-emptying time	1590:1621	Compared to the control, only 20RMD-ER had lower gastric half-emptying time and 20CB-ER had higher subject satisfaction.					
31328214	1	9	theme	index	151:155	arg1	food					162:165	a high glycemic index (GI) food	135:165	a high glycemic index (GI) food	135:165	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	7	10	theme	20SPI-ER	1126:1133	arg1	end-point					1058:1066	The end-point	1054:1066	The end-point of the second-phase digestion (C∞2)	1054:1102	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	7	10	theme	20SPI-ER	1126:1133	arg1	lower					1140:1144	lower	1140:1144	lower	1140:1144	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	7	10	theme	20SPI-ER	1126:1133	arg1	eGI					1112:1114	the eGI	1108:1114	the eGI of cooked 20SPI-ER	1108:1133	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	10	11	theme	subject	1646:1652	arg1	satisfaction					1654:1665	higher subject satisfaction	1639:1665	higher subject satisfaction	1639:1665	Compared to the control, only 20RMD-ER had lower gastric half-emptying time and 20CB-ER had higher subject satisfaction.					
31328214	2	12	theme	gastric	418:424	arg1	emptying					426:433	human gastric emptying rate and satiety scoring	412:458	emptying	426:433	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	3	13	theme	extruded	625:632	arg1	rice					634:637	20% supplemented extruded rice	608:637	20% supplemented extruded rice	608:637	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	4	14	theme	cooked	766:771	arg1	rice					782:785	cooked extruded rice	766:785	cooked extruded rice	766:785	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	3	15	theme	estimated	658:666	arg1	eGI					684:686	eGI	684:686	eGI	684:686	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	3	15	theme	estimated	658:666	arg1	index					677:681	the estimated glycemic index	654:681	the estimated glycemic index (eGI)	654:687	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	1	16	theme	extruded	171:178	arg1	rice					189:192	extruded reformed rice	171:192	extruded reformed rice	171:192	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	4	17	theme	rate	744:747	arg1	constant					749:756	the first-phase rate constant	728:756	the first-phase rate constant (k1) of cooked extruded rice	728:785	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	4	17	theme	rate	744:747	arg1	higher					791:796	higher	791:796	higher	791:796	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	4	17	theme	rate	744:747	arg1	k1					759:760	k1	759:760	k1	759:760	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	9	18	theme	[single	1442:1448	arg1	phase					1450:1454	[single phase	1442:1454	[single phase	1442:1454	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	5	19	theme	corn	846:849	arg1	bran					851:854	20% corn bran	842:854	20% corn bran extruded rice (20CB-ER)	842:878	Conversely, for 20% corn bran extruded rice (20CB-ER), a low value of k1 and high for k2 and C∞2 were found.					
31328214	2	20	theme	satiety	444:450	arg1	scoring					452:458	satiety scoring	444:458	satiety scoring	444:458	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	6	21	theme	rice	956:959	arg1	20RMD-ER					962:969	The 20% RMD extruded rice (20RMD-ER)	935:970	The 20% RMD extruded rice (20RMD-ER)	935:970	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	6	22	theme	similar	1036:1042	arg1	values					1046:1051	similar k values	1036:1051	similar k values	1036:1051	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	7	23	theme	second-phase	1075:1086	arg1	C∞2					1099:1101	C∞2	1099:1101	C∞2	1099:1101	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	7	23	theme	second-phase	1075:1086	arg1	digestion					1088:1096	the second-phase digestion	1071:1096	the second-phase digestion (C∞2)	1071:1102	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	5	24	theme	k1	896:897	arg1	value					887:891	a low value	881:891	a low value of k1 and high for k2 and C∞2	881:921	Conversely, for 20% corn bran extruded rice (20CB-ER), a low value of k1 and high for k2 and C∞2 were found.					
31328214	3	25	theme	simulated	492:500	arg1	system					502:507	a human simulated system	484:507	a human simulated system	484:507	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	0	26	theme	different	66:74	arg1	ways					76:79	different ways	66:79	different ways with added protein or fiber	66:107	Starch digestion kinetics of extruded reformed rice is changed in different ways with added protein or fiber.					
31328214	5	27	theme	high	903:906	arg1	value					887:891	a low value	881:891	a low value of k1 and high for k2 and C∞2	881:921	Conversely, for 20% corn bran extruded rice (20CB-ER), a low value of k1 and high for k2 and C∞2 were found.					
31328214	1	28	theme	high	137:140	arg1	GI					158:159	GI	158:159	GI	158:159	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	1	28	theme	high	137:140	arg1	index					151:155	a high glycemic index	135:155	a high glycemic index (GI) food	135:165	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	4	29	theme	rice	782:785	arg1	constant					749:756	the first-phase rate constant	728:756	the first-phase rate constant (k1) of cooked extruded rice	728:785	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	4	29	theme	rice	782:785	arg1	higher					791:796	higher	791:796	higher	791:796	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	4	29	theme	rice	782:785	arg1	k1					759:760	k1	759:760	k1	759:760	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	2	30	dep	isolate	297:303	arg1	bran					335:338	[corn bran	329:338	[corn bran	329:338	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	31	theme	fibers	322:327	arg1	effect					269:274	the effect	265:274	the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring	265:458	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	0	32	theme	Starch	0:5	arg1	kinetics					17:24	Starch digestion kinetics	0:24	Starch digestion kinetics of extruded reformed rice	0:50	Starch digestion kinetics of extruded reformed rice is changed in different ways with added protein or fiber.					
31328214	1	33	theme	lower	229:233	arg1	product					238:244	a lower GI product	227:244	a lower GI product	227:244	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	9	34	theme	two-phase	1460:1468	arg1	high/low					1478:1485	two-phase (k1/k2) high/low	1460:1485	two-phase (k1/k2) high/low	1460:1485	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	9	34	theme	two-phase	1460:1468	arg1	low/high					1488:1495	low/high	1488:1495	low/high	1488:1495	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	6	35	theme	RMD	943:945	arg1	20RMD-ER					962:969	The 20% RMD extruded rice (20RMD-ER)	935:970	The 20% RMD extruded rice (20RMD-ER)	935:970	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	8	36	theme	incorporated	1263:1274	arg1	kernels					1276:1282	the SPI and CB incorporated kernels	1248:1282	the SPI and CB incorporated kernels	1248:1282	These differences were speculated to be due to the heterogeneous matrices of the SPI and CB incorporated kernels, opposed to the homogeneous matrices of the RMD and control.					
31328214	0	37	theme	extruded	29:36	arg1	rice					47:50	extruded reformed rice	29:50	extruded reformed rice	29:50	Starch digestion kinetics of extruded reformed rice is changed in different ways with added protein or fiber.					
31328214	9	38	theme	added	1523:1527	arg1	protein					1529:1535	added protein	1523:1535	added protein	1523:1535	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	3	39	theme	single-phase	518:529	arg1	pattern					539:545	a single-phase kinetic pattern	516:545	a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI)	516:687	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	4	40	theme	%	696:696	arg1	20SPI-ER					717:724	20% SPI extruded rice (20SPI-ER)	694:725	20% SPI extruded rice (20SPI-ER)	694:725	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	2	41	theme	soy	285:287	arg1	SPI					306:308	SPI	306:308	SPI	306:308	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	41	theme	soy	285:287	arg1	isolate					297:303	added soy protein isolate	279:303	added soy protein isolate (SPI)	279:309	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	41	theme	soy	285:287	arg1	maltodextrin					356:367	resistant maltodextrin	346:367	resistant maltodextrin (RMD)]	346:374	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	41	theme	soy	285:287	arg1	CB					341:342	CB	341:342	CB	341:342	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	0	42	theme	rice	47:50	arg1	kinetics					17:24	Starch digestion kinetics	0:24	Starch digestion kinetics of extruded reformed rice	0:50	Starch digestion kinetics of extruded reformed rice is changed in different ways with added protein or fiber.					
31328214	5	43	theme	%	844:844	arg1	bran					851:854	20% corn bran	842:854	20% corn bran extruded rice (20CB-ER)	842:878	Conversely, for 20% corn bran extruded rice (20CB-ER), a low value of k1 and high for k2 and C∞2 were found.					
31328214	6	44	theme	%	941:941	arg1	20RMD-ER					962:969	The 20% RMD extruded rice (20RMD-ER)	935:970	The 20% RMD extruded rice (20RMD-ER)	935:970	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	1	45	theme	reformed	180:187	arg1	rice					189:192	extruded reformed rice	171:192	extruded reformed rice	171:192	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	2	46	dep	in	385:386	arg1	vitro					388:392	vitro	388:392	vitro	388:392	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	8	47	theme	homogeneous	1300:1310	arg1	matrices					1312:1319	the homogeneous matrices	1296:1319	the homogeneous matrices of the RMD and control	1296:1342	These differences were speculated to be due to the heterogeneous matrices of the SPI and CB incorporated kernels, opposed to the homogeneous matrices of the RMD and control.					
31328214	3	48	theme	regular	551:557	arg1	rice					568:571	regular reformed rice	551:571	regular reformed rice	551:571	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	6	49	with	digestion	1021:1029	arg1	values					1046:1051	similar k values	1036:1051	similar k values	1036:1051	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	10	50	theme	gastric	1596:1602	arg1	time					1618:1621	lower gastric half-emptying time	1590:1621	lower gastric half-emptying time	1590:1621	Compared to the control, only 20RMD-ER had lower gastric half-emptying time and 20CB-ER had higher subject satisfaction.					
31328214	2	51	theme	isolate	297:303	arg1	effect					269:274	the effect	265:274	the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring	265:458	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	8	52	theme	RMD	1328:1330	arg1	matrices					1312:1319	the homogeneous matrices	1296:1319	the homogeneous matrices of the RMD and control	1296:1342	These differences were speculated to be due to the heterogeneous matrices of the SPI and CB incorporated kernels, opposed to the homogeneous matrices of the RMD and control.					
31328214	3	53	theme	two-phase	577:585	arg1	patterns					595:602	two-phase kinetic patterns	577:602	two-phase kinetic patterns	577:602	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	6	54	theme	kinetic	1013:1019	arg1	digestion					1021:1029	a single-phase kinetic digestion	998:1029	a single-phase kinetic digestion with similar k values	998:1051	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	9	55	theme	rice	1360:1363	arg1	compositions					1365:1376	extruded rice compositions	1351:1376	extruded rice compositions	1351:1376	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	9	56	theme	different	1406:1414	arg1	kinetics					1433:1440	different starch digestion kinetics	1406:1440	different starch digestion kinetics	1406:1440	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	7	57	theme	fiber	1155:1159	arg1	addition					1161:1168	fiber addition	1155:1168	fiber addition	1155:1168	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	8	58	theme	control	1336:1342	arg1	matrices					1312:1319	the homogeneous matrices	1296:1319	the homogeneous matrices of the RMD and control	1296:1342	These differences were speculated to be due to the heterogeneous matrices of the SPI and CB incorporated kernels, opposed to the homogeneous matrices of the RMD and control.					
31328214	3	59	theme	supplemented	612:623	arg1	rice					634:637	20% supplemented extruded rice	608:637	20% supplemented extruded rice	608:637	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	9	60	with	eGI	1514:1516	arg1	fiber					1540:1544	fiber	1540:1544	fiber	1540:1544	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	9	60	with	eGI	1514:1516	arg1	protein					1529:1535	added protein	1523:1535	added protein	1523:1535	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	1	61	theme	glycemic	142:149	arg1	GI					158:159	GI	158:159	GI	158:159	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	1	61	theme	glycemic	142:149	arg1	index					151:155	a high glycemic index	135:155	a high glycemic index (GI) food	135:165	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	2	62	dep	emptying	426:433	arg1	rate					435:438	rate	435:438	rate	435:438	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	4	63	theme	SPI	698:700	arg1	20SPI-ER					717:724	20% SPI extruded rice (20SPI-ER)	694:725	20% SPI extruded rice (20SPI-ER)	694:725	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	10	64	contain	had	1586:1588	arg2	time					1618:1621	lower gastric half-emptying time	1590:1621	lower gastric half-emptying time	1590:1621	Compared to the control, only 20RMD-ER had lower gastric half-emptying time and 20CB-ER had higher subject satisfaction.					
31328214	10	64	contain	had	1586:1588	arg1	20RMD-ER					1577:1584	only 20RMD-ER	1572:1584	only 20RMD-ER	1572:1584	Compared to the control, only 20RMD-ER had lower gastric half-emptying time and 20CB-ER had higher subject satisfaction.					
31328214	3	65	theme	20	608:609	arg1	%					610:610	%	610:610	%	610:610	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	4	66	theme	rice	711:714	arg1	20SPI-ER					717:724	20% SPI extruded rice (20SPI-ER)	694:725	20% SPI extruded rice (20SPI-ER)	694:725	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	10	67	theme	higher	1639:1644	arg1	satisfaction					1654:1665	higher subject satisfaction	1639:1665	higher subject satisfaction	1639:1665	Compared to the control, only 20RMD-ER had lower gastric half-emptying time and 20CB-ER had higher subject satisfaction.					
31328214	7	68	theme	cooked	1119:1124	arg1	20SPI-ER					1126:1133	cooked 20SPI-ER	1119:1133	cooked 20SPI-ER	1119:1133	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	10	69	contain	had	1635:1637	arg1	20CB-ER					1627:1633	20CB-ER	1627:1633	20CB-ER	1627:1633	Compared to the control, only 20RMD-ER had lower gastric half-emptying time and 20CB-ER had higher subject satisfaction.					
31328214	10	69	contain	had	1635:1637	arg2	satisfaction					1654:1665	higher subject satisfaction	1639:1665	higher subject satisfaction	1639:1665	Compared to the control, only 20RMD-ER had lower gastric half-emptying time and 20CB-ER had higher subject satisfaction.					
31328214	2	70	theme	human	412:416	arg1	emptying					426:433	human gastric emptying rate and satiety scoring	412:458	emptying	426:433	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	3	71	theme	glycemic	668:675	arg1	eGI					684:686	eGI	684:686	eGI	684:686	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	3	71	theme	glycemic	668:675	arg1	index					677:681	the estimated glycemic index	654:681	the estimated glycemic index (eGI)	654:687	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	3	72	theme	Starch	461:466	arg1	digestion					468:476	Starch digestion	461:476	Starch digestion using a human simulated system	461:507	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	4	73	theme	first-phase	732:742	arg1	constant					749:756	the first-phase rate constant	728:756	the first-phase rate constant (k1) of cooked extruded rice	728:785	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	4	73	theme	first-phase	732:742	arg1	higher					791:796	higher	791:796	higher	791:796	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	4	73	theme	first-phase	732:742	arg1	k1					759:760	k1	759:760	k1	759:760	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	6	74	theme	k	1044:1044	arg1	values					1046:1051	similar k values	1036:1051	similar k values	1036:1051	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	1	75	theme	White	110:114	arg1	rice					116:119	White rice	110:119	White rice	110:119	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	6	76	theme	extruded	947:954	arg1	20RMD-ER					962:969	The 20% RMD extruded rice (20RMD-ER)	935:970	The 20% RMD extruded rice (20RMD-ER)	935:970	The 20% RMD extruded rice (20RMD-ER) and the control exhibited a single-phase kinetic digestion with similar k values.					
31328214	3	77	theme	human	486:490	arg1	system					502:507	a human simulated system	484:507	a human simulated system	484:507	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	2	78	theme	in	385:386	arg1	digestion					394:402	in vitro digestion	385:402	in vitro digestion	385:402	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	7	79	theme	digestion	1088:1096	arg1	end-point					1058:1066	The end-point	1054:1066	The end-point of the second-phase digestion (C∞2)	1054:1102	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	7	79	theme	digestion	1088:1096	arg1	lower					1140:1144	lower	1140:1144	lower	1140:1144	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	7	79	theme	digestion	1088:1096	arg1	eGI					1112:1114	the eGI	1108:1114	the eGI of cooked 20SPI-ER	1108:1133	The end-point of the second-phase digestion (C∞2) and the eGI of cooked 20SPI-ER were lower than for fiber addition.					
31328214	9	80	theme	k1/k2	1471:1475	arg1	high/low					1478:1485	two-phase (k1/k2) high/low	1460:1485	two-phase (k1/k2) high/low	1460:1485	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	9	80	theme	k1/k2	1471:1475	arg1	low/high					1488:1495	low/high	1488:1495	low/high	1488:1495	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	8	81	theme	heterogeneous	1222:1234	arg1	matrices					1236:1243	the heterogeneous matrices	1218:1243	the heterogeneous matrices of the SPI and CB incorporated kernels	1218:1282	These differences were speculated to be due to the heterogeneous matrices of the SPI and CB incorporated kernels, opposed to the homogeneous matrices of the RMD and control.					
31328214	0	82	theme	added	86:90	arg1	protein					92:98	protein	92:98	protein	92:98	Starch digestion kinetics of extruded reformed rice is changed in different ways with added protein or fiber.					
31328214	8	83	dep	RMD	1328:1330	arg1	the					1324:1326	the	1324:1326	the	1324:1326	These differences were speculated to be due to the heterogeneous matrices of the SPI and CB incorporated kernels, opposed to the homogeneous matrices of the RMD and control.					
31328214	4	84	theme	extruded	773:780	arg1	rice					782:785	cooked extruded rice	766:785	cooked extruded rice	766:785	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	5	85	theme	low	883:885	arg1	value					887:891	a low value	881:891	a low value of k1 and high for k2 and C∞2	881:921	Conversely, for 20% corn bran extruded rice (20CB-ER), a low value of k1 and high for k2 and C∞2 were found.					
31328214	8	86	theme	SPI	1252:1254	arg1	kernels					1276:1282	the SPI and CB incorporated kernels	1248:1282	the SPI and CB incorporated kernels	1248:1282	These differences were speculated to be due to the heterogeneous matrices of the SPI and CB incorporated kernels, opposed to the homogeneous matrices of the RMD and control.					
31328214	2	87	theme	dietary	314:320	arg1	fibers					322:327	dietary fibers	314:327	dietary fibers	314:327	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	0	88	theme	digestion	7:15	arg1	kinetics					17:24	Starch digestion kinetics	0:24	Starch digestion kinetics of extruded reformed rice	0:50	Starch digestion kinetics of extruded reformed rice is changed in different ways with added protein or fiber.					
31328214	2	89	theme	resistant	346:354	arg1	RMD					370:372	RMD	370:372	RMD	370:372	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	89	theme	resistant	346:354	arg1	maltodextrin					356:367	resistant maltodextrin	346:367	resistant maltodextrin (RMD)]	346:374	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	89	theme	resistant	346:354	arg1	isolate					297:303	added soy protein isolate	279:303	added soy protein isolate (SPI)	279:309	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	8	90	theme	CB	1260:1261	arg1	kernels					1276:1282	the SPI and CB incorporated kernels	1248:1282	the SPI and CB incorporated kernels	1248:1282	These differences were speculated to be due to the heterogeneous matrices of the SPI and CB incorporated kernels, opposed to the homogeneous matrices of the RMD and control.					
31328214	2	91	dep	effect	269:274	arg1	emptying					426:433	human gastric emptying rate and satiety scoring	412:458	emptying	426:433	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	91	dep	effect	269:274	arg1	delay					379:383	delay	379:383	to delay in vitro digestion	376:402	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	1	92	theme	GI	235:236	arg1	product					238:244	a lower GI product	227:244	a lower GI product	227:244	White rice is considered a high glycemic index (GI) food and extruded reformed rice offers the opportunity to design a lower GI product.					
31328214	9	93	theme	medium	1507:1512	arg1	eGI					1514:1516	medium eGI	1507:1516	medium eGI	1507:1516	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	8	94	theme	kernels	1276:1282	arg1	matrices					1236:1243	the heterogeneous matrices	1218:1243	the heterogeneous matrices of the SPI and CB incorporated kernels	1218:1282	These differences were speculated to be due to the heterogeneous matrices of the SPI and CB incorporated kernels, opposed to the homogeneous matrices of the RMD and control.					
31328214	3	95	theme	kinetic	531:537	arg1	pattern					539:545	a single-phase kinetic pattern	516:545	a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI)	516:687	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	0	96	theme	reformed	38:45	arg1	rice					47:50	extruded reformed rice	29:50	extruded reformed rice	29:50	Starch digestion kinetics of extruded reformed rice is changed in different ways with added protein or fiber.					
31328214	2	97	theme	[corn	329:333	arg1	bran					335:338	[corn bran	329:338	[corn bran	329:338	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	5	98	theme	20	842:843	arg1	%					844:844	%	844:844	%	844:844	Conversely, for 20% corn bran extruded rice (20CB-ER), a low value of k1 and high for k2 and C∞2 were found.					
31328214	9	99	theme	starch	1416:1421	arg1	kinetics					1433:1440	different starch digestion kinetics	1406:1440	different starch digestion kinetics	1406:1440	Thus, extruded rice compositions were found that resulted in different starch digestion kinetics [single phase and two-phase (k1/k2) high/low, low/high] and were medium eGI with added protein or fiber.					
31328214	10	100	theme	lower	1590:1594	arg1	time					1618:1621	lower gastric half-emptying time	1590:1621	lower gastric half-emptying time	1590:1621	Compared to the control, only 20RMD-ER had lower gastric half-emptying time and 20CB-ER had higher subject satisfaction.					
31328214	3	101	theme	reformed	559:566	arg1	rice					568:571	regular reformed rice	551:571	regular reformed rice	551:571	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31328214	4	102	theme	20	694:695	arg1	%					696:696	%	696:696	%	696:696	For 20% SPI extruded rice (20SPI-ER), the first-phase rate constant (k1) of cooked extruded rice was higher than its second-phase (k2).					
31328214	2	103	theme	added	279:283	arg1	SPI					306:308	SPI	306:308	SPI	306:308	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	103	theme	added	279:283	arg1	isolate					297:303	added soy protein isolate	279:303	added soy protein isolate (SPI)	279:309	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	103	theme	added	279:283	arg1	maltodextrin					356:367	resistant maltodextrin	346:367	resistant maltodextrin (RMD)]	346:374	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	2	103	theme	added	279:283	arg1	CB					341:342	CB	341:342	CB	341:342	This study tested the effect of added soy protein isolate (SPI) or dietary fibers [corn bran (CB), resistant maltodextrin (RMD)] to delay in vitro digestion, and on human gastric emptying rate and satiety scoring.					
31328214	3	104	theme	kinetic	587:593	arg1	patterns					595:602	two-phase kinetic patterns	577:602	two-phase kinetic patterns	577:602	Starch digestion using a human simulated system showed a single-phase kinetic pattern for regular reformed rice and two-phase kinetic patterns for 20% supplemented extruded rice, which lowered the estimated glycemic index (eGI).					
31482162	8	0	theme	glycerol-free	1381:1393	arg1	cryopreservation					1395:1410	the glycerol-free cryopreservation	1377:1410	the glycerol-free cryopreservation of cells and tissues in biomedicine	1377:1446	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.					
31482162	5	1	theme	membrane	794:801	arg1	stabilization					803:815	membrane stabilization	794:815	membrane stabilization	794:815	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	7	2	theme	osmotic	1166:1172	arg1	stress					1174:1179	osmotic stress	1166:1179	osmotic stress	1166:1179	Thus, membrane protection for preventing osmotic stress and ice injury could be boosted via synergistic interaction between the glycopeptide and trehalose.					
31482162	1	3	theme	osmotic	173:179	arg1	stress					181:186	osmotic stress	173:186	osmotic stress	173:186	The long-term cryopreservation of cells, tissues and organs is limited by both osmotic stress and ice injury during freezing or thawing.					
31482162	4	4	theme	enhanced	644:651	arg1	cryosurvival					653:664	enhanced cryosurvival	644:664	enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	644:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	3	5	theme	glyco	428:432	arg1	peptides					434:441	trehalose-functional (glyco)peptides	406:441	trehalose-functional (glyco)peptides	406:441	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	4	6	dep	%	728:728	arg1	to					715:716	to	715:716	to	715:716	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	7	theme	trehalose	752:760	arg1	molecules					762:770	trehalose molecules	752:770	trehalose molecules	752:770	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	8	theme	sheep	669:673	arg1	RBCs					692:695	RBCs	692:695	RBCs	692:695	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	8	theme	sheep	669:673	arg1	cells					685:689	sheep red blood cells	669:689	sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	669:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	9	from	cryosurvival	653:664	arg1	%					728:728	49.1 ± 0.9% to 75.0 ± 2.4%	703:728	49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	703:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	8	10	theme	biocompatible	1303:1315	arg1	glycopeptides					1317:1329	biocompatible glycopeptides	1303:1329	biocompatible glycopeptides	1303:1329	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.					
31482162	2	11	theme	cell	300:303	arg1	cryopreservation					305:320	cell cryopreservation	300:320	cell cryopreservation based on membrane stabilization of glycopeptides and trehalose	300:383	Introduction of biocompatible cryoprotective agents is promising for cell cryopreservation based on membrane stabilization of glycopeptides and trehalose.					
31482162	5	12	theme	osmotic	941:947	arg1	injury					949:954	osmotic injury	941:954	osmotic injury	941:954	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	3	13	theme	tethering	476:484	arg1	-l-arginine					508:518	successively tethering Nω-(4-toluenesulfonyl)-l-arginine	463:518	successively tethering Nω-(4-toluenesulfonyl)-l-arginine	463:518	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	3	14	theme	peptides	434:441	arg1	series					396:401	a series	394:401	a series of trehalose-functional (glyco)peptides	394:441	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	5	15	theme	cell	922:925	arg1	membrane					927:934	the cell membrane	918:934	the cell membrane	918:934	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	7	16	theme	membrane	1131:1138	arg1	protection					1140:1149	membrane protection	1131:1149	membrane protection for preventing osmotic stress and ice injury	1131:1194	Thus, membrane protection for preventing osmotic stress and ice injury could be boosted via synergistic interaction between the glycopeptide and trehalose.					
31482162	8	17	from	cryopreservation	1395:1410	arg1	biomedicine					1436:1446	biomedicine	1436:1446	biomedicine	1436:1446	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.					
31482162	1	18	theme	long-term	98:106	arg1	cryopreservation					108:123	The long-term cryopreservation	94:123	The long-term cryopreservation of cells, tissues and organs	94:152	The long-term cryopreservation of cells, tissues and organs is limited by both osmotic stress and ice injury during freezing or thawing.					
31482162	3	19	theme	Nω-	486:488	arg1	-l-arginine					508:518	successively tethering Nω-(4-toluenesulfonyl)-l-arginine	463:518	successively tethering Nω-(4-toluenesulfonyl)-l-arginine	463:518	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	7	20	gly	glycopeptide	1253:1264	arg2	glycopeptide					1253:1264	glycopeptide	1253:1264	glycopeptide	1253:1264	Thus, membrane protection for preventing osmotic stress and ice injury could be boosted via synergistic interaction between the glycopeptide and trehalose.					
31482162	6	21	theme	recrystallization	1086:1102	arg1	inhibition					1104:1113	ice recrystallization inhibition	1082:1113	ice recrystallization inhibition activity	1082:1122	Meanwhile, trehalose could prevent RBCs from ice injury via ice recrystallization inhibition activity.					
31482162	1	22	theme	ice	192:194	arg1	injury					196:201	ice injury	192:201	ice injury	192:201	The long-term cryopreservation of cells, tissues and organs is limited by both osmotic stress and ice injury during freezing or thawing.					
31482162	0	23	theme	Trehalose-functional	0:19	arg1	glycopeptide					21:32	Trehalose-functional glycopeptide	0:32	Trehalose-functional glycopeptide	0:32	Trehalose-functional glycopeptide enhances glycerol-free cryopreservation of red blood cells.					
31482162	4	24	theme	synthesized	606:616	arg1	glycopeptide					618:629	The specifically synthesized glycopeptide	589:629	The specifically synthesized glycopeptide	589:629	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	8	25	theme	combined	1285:1292	arg1	usage					1294:1298	The combined usage	1281:1298	The combined usage of biocompatible glycopeptides and trehalose	1281:1343	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.					
31482162	5	26	theme	electrostatic	959:971	arg1	interactions					973:984	electrostatic interactions	959:984	electrostatic interactions	959:984	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	5	26	theme	electrostatic	959:971	arg1	stacking					991:998	π-π stacking	987:998	π-π stacking	987:998	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	5	26	theme	electrostatic	959:971	arg1	bonding					1013:1019	hydrogen bonding	1004:1019	hydrogen bonding	1004:1019	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	3	27	theme	successively	463:474	arg1	-l-arginine					508:518	successively tethering Nω-(4-toluenesulfonyl)-l-arginine	463:518	successively tethering Nω-(4-toluenesulfonyl)-l-arginine	463:518	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	0	28	theme	glycerol-free	43:55	arg1	cryopreservation					57:72	glycerol-free cryopreservation	43:72	glycerol-free cryopreservation of red blood cells	43:91	Trehalose-functional glycopeptide enhances glycerol-free cryopreservation of red blood cells.					
31482162	5	29	dep	attach	863:868	arg1	such					830:833	such	830:833	such	830:833	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	7	30	dep	glycopeptide	1253:1264	arg1	the					1249:1251	the	1249:1251	the	1249:1251	Thus, membrane protection for preventing osmotic stress and ice injury could be boosted via synergistic interaction between the glycopeptide and trehalose.					
31482162	6	31	from	injury	1071:1076	arg1	RBCs					1057:1060	RBCs	1057:1060	RBCs from ice injury	1057:1076	Meanwhile, trehalose could prevent RBCs from ice injury via ice recrystallization inhibition activity.					
31482162	2	32	theme	membrane	331:338	arg1	stabilization					340:352	membrane stabilization	331:352	membrane stabilization of glycopeptides and trehalose	331:383	Introduction of biocompatible cryoprotective agents is promising for cell cryopreservation based on membrane stabilization of glycopeptides and trehalose.					
31482162	6	33	theme	inhibition	1104:1113	arg1	activity					1115:1122	ice recrystallization inhibition activity	1082:1122	ice recrystallization inhibition activity	1082:1122	Meanwhile, trehalose could prevent RBCs from ice injury via ice recrystallization inhibition activity.					
31482162	8	34	theme	glycopeptides	1317:1329	arg1	usage					1294:1298	The combined usage	1281:1298	The combined usage of biocompatible glycopeptides and trehalose	1281:1343	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.					
31482162	3	35	theme	trehalose-functional	406:425	arg1	peptides					434:441	trehalose-functional (glyco)peptides	406:441	trehalose-functional (glyco)peptides	406:441	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	8	36	theme	tissues	1425:1431	arg1	cryopreservation					1395:1410	the glycerol-free cryopreservation	1377:1410	the glycerol-free cryopreservation of cells and tissues in biomedicine	1377:1446	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.					
31482162	3	37	theme	side	554:557	arg1	ε-polylysine					575:586	ε-polylysine	575:586	ε-polylysine	575:586	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	3	37	theme	side	554:557	arg1	groups					565:570	the side amino groups	550:570	the side amino groups of ε-polylysine	550:586	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	4	38	theme	±	723:723	arg1	%					728:728	49.1 ± 0.9% to 75.0 ± 2.4%	703:728	49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	703:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	7	39	theme	ice	1185:1187	arg1	injury					1189:1194	ice injury	1185:1194	ice injury	1185:1194	Thus, membrane protection for preventing osmotic stress and ice injury could be boosted via synergistic interaction between the glycopeptide and trehalose.					
31482162	8	40	theme	trehalose	1335:1343	arg1	usage					1294:1298	The combined usage	1281:1298	The combined usage of biocompatible glycopeptides and trehalose	1281:1343	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.					
31482162	2	41	theme	biocompatible	247:259	arg1	agents					276:281	biocompatible cryoprotective agents	247:281	biocompatible cryoprotective agents	247:281	Introduction of biocompatible cryoprotective agents is promising for cell cryopreservation based on membrane stabilization of glycopeptides and trehalose.					
31482162	4	42	theme	%	713:713	arg1	%					728:728	49.1 ± 0.9% to 75.0 ± 2.4%	703:728	49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	703:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	6	43	theme	ice	1067:1069	arg1	injury					1071:1076	ice injury	1067:1076	ice injury	1067:1076	Meanwhile, trehalose could prevent RBCs from ice injury via ice recrystallization inhibition activity.					
31482162	5	44	theme	membrane	882:889	arg1	surface					891:897	the cell membrane surface	873:897	the cell membrane surface of RBCs	873:905	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	0	45	gly	glycopeptide	21:32	arg2	glycopeptide					21:32	Trehalose-functional glycopeptide	0:32	Trehalose-functional glycopeptide	0:32	Trehalose-functional glycopeptide enhances glycerol-free cryopreservation of red blood cells.					
31482162	3	46	theme	4-toluenesulfonyl	490:506	arg1	-l-arginine					508:518	successively tethering Nω-(4-toluenesulfonyl)-l-arginine	463:518	successively tethering Nω-(4-toluenesulfonyl)-l-arginine	463:518	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	0	47	theme	blood	81:85	arg1	cells					87:91	red blood cells	77:91	red blood cells	77:91	Trehalose-functional glycopeptide enhances glycerol-free cryopreservation of red blood cells.					
31482162	8	48	theme	cells	1415:1419	arg1	cryopreservation					1395:1410	the glycerol-free cryopreservation	1377:1410	the glycerol-free cryopreservation of cells and tissues in biomedicine	1377:1446	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.					
31482162	1	49	theme	tissues	135:141	arg1	cryopreservation					108:123	The long-term cryopreservation	94:123	The long-term cryopreservation of cells, tissues and organs	94:152	The long-term cryopreservation of cells, tissues and organs is limited by both osmotic stress and ice injury during freezing or thawing.					
31482162	0	50	theme	red	77:79	arg1	cells					87:91	red blood cells	77:91	red blood cells	77:91	Trehalose-functional glycopeptide enhances glycerol-free cryopreservation of red blood cells.					
31482162	6	51	theme	ice	1082:1084	arg1	inhibition					1104:1113	ice recrystallization inhibition	1082:1113	ice recrystallization inhibition activity	1082:1122	Meanwhile, trehalose could prevent RBCs from ice injury via ice recrystallization inhibition activity.					
31482162	5	52	gly	glycopeptide	844:855	arg2	glycopeptide					844:855	the glycopeptide	840:855	the glycopeptide	840:855	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	4	53	gly	glycopeptide	618:629	arg2	glycopeptide					618:629	The specifically synthesized glycopeptide	589:629	The specifically synthesized glycopeptide	589:629	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	2	54	theme	trehalose	375:383	arg1	stabilization					340:352	membrane stabilization	331:352	membrane stabilization of glycopeptides and trehalose	331:383	Introduction of biocompatible cryoprotective agents is promising for cell cryopreservation based on membrane stabilization of glycopeptides and trehalose.					
31482162	2	55	gly	glycopeptides	357:369	arg2	glycopeptides					357:369	glycopeptides	357:369	glycopeptides	357:369	Introduction of biocompatible cryoprotective agents is promising for cell cryopreservation based on membrane stabilization of glycopeptides and trehalose.					
31482162	5	56	theme	RBCs	902:905	arg1	surface					891:897	the cell membrane surface	873:897	the cell membrane surface of RBCs	873:905	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	1	57	theme	organs	147:152	arg1	cryopreservation					108:123	The long-term cryopreservation	94:123	The long-term cryopreservation of cells, tissues and organs	94:152	The long-term cryopreservation of cells, tissues and organs is limited by both osmotic stress and ice injury during freezing or thawing.					
31482162	1	58	theme	cells	128:132	arg1	cryopreservation					108:123	The long-term cryopreservation	94:123	The long-term cryopreservation of cells, tissues and organs	94:152	The long-term cryopreservation of cells, tissues and organs is limited by both osmotic stress and ice injury during freezing or thawing.					
31482162	4	59	theme	blood	679:683	arg1	RBCs					692:695	RBCs	692:695	RBCs	692:695	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	59	theme	blood	679:683	arg1	cells					685:689	sheep red blood cells	669:689	sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	669:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	3	60	theme	ε-polylysine	575:586	arg1	ε-polylysine					575:586	ε-polylysine	575:586	ε-polylysine	575:586	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	3	60	theme	ε-polylysine	575:586	arg1	groups					565:570	the side amino groups	550:570	the side amino groups of ε-polylysine	550:586	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	5	61	theme	π-π	987:989	arg1	interactions					973:984	electrostatic interactions	959:984	electrostatic interactions	959:984	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	5	61	theme	π-π	987:989	arg1	stacking					991:998	π-π stacking	987:998	π-π stacking	987:998	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	2	62	theme	glycopeptides	357:369	arg1	stabilization					340:352	membrane stabilization	331:352	membrane stabilization of glycopeptides and trehalose	331:383	Introduction of biocompatible cryoprotective agents is promising for cell cryopreservation based on membrane stabilization of glycopeptides and trehalose.					
31482162	4	63	from	pH	733:734	arg1	%					728:728	49.1 ± 0.9% to 75.0 ± 2.4%	703:728	49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	703:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	64	theme	red	675:677	arg1	RBCs					692:695	RBCs	692:695	RBCs	692:695	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	64	theme	red	675:677	arg1	cells					685:689	sheep red blood cells	669:689	sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	669:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	8	65	gly	glycopeptides	1317:1329	arg2	glycopeptides					1317:1329	biocompatible glycopeptides	1303:1329	biocompatible glycopeptides	1303:1329	The combined usage of biocompatible glycopeptides and trehalose could potentially be applied in the glycerol-free cryopreservation of cells and tissues in biomedicine.					
31482162	5	66	theme	cell	877:880	arg1	surface					891:897	the cell membrane surface	873:897	the cell membrane surface of RBCs	873:905	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	2	67	theme	agents	276:281	arg1	Introduction					231:242	Introduction	231:242	Introduction of biocompatible cryoprotective agents	231:281	Introduction of biocompatible cryoprotective agents is promising for cell cryopreservation based on membrane stabilization of glycopeptides and trehalose.					
31482162	3	68	theme	amino	559:563	arg1	ε-polylysine					575:586	ε-polylysine	575:586	ε-polylysine	575:586	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	3	68	theme	amino	559:563	arg1	groups					565:570	the side amino groups	550:570	the side amino groups of ε-polylysine	550:586	Herein, a series of trehalose-functional (glyco)peptides were synthesized by successively tethering Nω-(4-toluenesulfonyl)-l-arginine and carboxylated trehalose to the side amino groups of ε-polylysine.					
31482162	4	69	theme	±	708:708	arg1	%					728:728	49.1 ± 0.9% to 75.0 ± 2.4%	703:728	49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	703:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	0	70	theme	cells	87:91	arg1	cryopreservation					57:72	glycerol-free cryopreservation	43:72	glycerol-free cryopreservation of red blood cells	43:91	Trehalose-functional glycopeptide enhances glycerol-free cryopreservation of red blood cells.					
31482162	7	71	theme	synergistic	1217:1227	arg1	interaction					1229:1239	synergistic interaction	1217:1239	synergistic interaction between the glycopeptide and trehalose	1217:1278	Thus, membrane protection for preventing osmotic stress and ice injury could be boosted via synergistic interaction between the glycopeptide and trehalose.					
31482162	2	72	theme	cryoprotective	261:274	arg1	agents					276:281	biocompatible cryoprotective agents	247:281	biocompatible cryoprotective agents	247:281	Introduction of biocompatible cryoprotective agents is promising for cell cryopreservation based on membrane stabilization of glycopeptides and trehalose.					
31482162	5	73	theme	hydrogen	1004:1011	arg1	interactions					973:984	electrostatic interactions	959:984	electrostatic interactions	959:984	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	5	73	theme	hydrogen	1004:1011	arg1	bonding					1013:1019	hydrogen bonding	1004:1019	hydrogen bonding	1004:1019	Cryopreservation via membrane stabilization was proposed such that the glycopeptide could attach on the cell membrane surface of RBCs to protect the cell membrane from osmotic injury by electrostatic interactions, π-π stacking and hydrogen bonding.					
31482162	4	74	from	%	728:728	arg1	RBCs					692:695	RBCs	692:695	RBCs	692:695	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	74	from	%	728:728	arg1	cells					685:689	sheep red blood cells	669:689	sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	669:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	74	from	%	728:728	arg1	cryosurvival					653:664	enhanced cryosurvival	644:664	enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	644:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
31482162	4	75	theme	cells	685:689	arg1	cryosurvival					653:664	enhanced cryosurvival	644:664	enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4	644:738	The specifically synthesized glycopeptide demonstrated enhanced cryosurvival of sheep red blood cells (RBCs) from 49.1 ± 0.9% to 75.0 ± 2.4% at pH 7.4, along with trehalose molecules.					
30822472	5	0	theme	structural	1026:1035	arg1	properties					1037:1046	their physical and structural properties	1007:1046	their physical and structural properties	1007:1046	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	3	1	with	films	647:651	arg1	characteristics					676:690	unique mechanical characteristics	658:690	unique mechanical characteristics	658:690	The results showed a direct relationship between the composition of the film and each physical property, providing films with unique mechanical characteristics and barrier properties.					
30822472	3	1	with	films	647:651	arg1	properties					704:713	barrier properties	696:713	barrier properties	696:713	The results showed a direct relationship between the composition of the film and each physical property, providing films with unique mechanical characteristics and barrier properties.					
30822472	4	2	from	phase	730:734	arg1	reformulated					752:763	reformulated	752:763	reformulated	752:763	In the second phase, the films were reformulated, maintaining the proportions of CP and BCM constant and altering the G content.					
30822472	4	2	from	phase	730:734	arg1	films					741:745	the films	737:745	the films	737:745	In the second phase, the films were reformulated, maintaining the proportions of CP and BCM constant and altering the G content.					
30822472	4	3	theme	second	723:728	arg1	phase					730:734	the second phase	719:734	the second phase	719:734	In the second phase, the films were reformulated, maintaining the proportions of CP and BCM constant and altering the G content.					
30822472	4	4	dep	CP	797:798	arg1	constant					808:815	constant	808:815	constant	808:815	In the second phase, the films were reformulated, maintaining the proportions of CP and BCM constant and altering the G content.					
30822472	2	5	theme	moisture	459:466	arg1	contents					468:475	their moisture contents	453:475	their moisture contents	453:475	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	5	6	theme	physical	1013:1020	arg1	properties					1037:1046	their physical and structural properties	1007:1046	their physical and structural properties	1007:1046	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	4	7	theme	CP	797:798	arg1	proportions					782:792	the proportions	778:792	the proportions of CP and BCM constant	778:815	In the second phase, the films were reformulated, maintaining the proportions of CP and BCM constant and altering the G content.					
30822472	1	8	theme	water	144:148	arg1	sorption					150:157	water sorption	144:157	water sorption	144:157	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	3	9	theme	unique	658:663	arg1	characteristics					676:690	unique mechanical characteristics	658:690	unique mechanical characteristics	658:690	The results showed a direct relationship between the composition of the film and each physical property, providing films with unique mechanical characteristics and barrier properties.					
30822472	3	10	theme	mechanical	665:674	arg1	characteristics					676:690	unique mechanical characteristics	658:690	unique mechanical characteristics	658:690	The results showed a direct relationship between the composition of the film and each physical property, providing films with unique mechanical characteristics and barrier properties.					
30822472	4	11	theme	BCM	804:806	arg1	proportions					782:792	the proportions	778:792	the proportions of CP and BCM constant	778:815	In the second phase, the films were reformulated, maintaining the proportions of CP and BCM constant and altering the G content.					
30822472	5	12	theme	temperatures	934:945	arg1	use					915:917	the use	911:917	the use of high drying temperatures (up to 70 °C) for the confection of films	911:987	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	5	13	theme	permeable	875:883	arg1	structures					885:894	permeable structures	875:894	permeable structures	875:894	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	0	14	theme	Physical	0:7	arg1	properties					9:18	Physical properties	0:18	Physical properties of films	0:27	Physical properties of films based on pectin and babassu coconut mesocarp.					
30822472	1	15	theme	babassu	221:227	arg1	mesocarp					237:244	babassu coconut mesocarp	221:244	babassu coconut mesocarp (BCM)	221:250	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	1	15	theme	babassu	221:227	arg1	BCM					247:249	BCM	247:249	BCM	247:249	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	5	16	dep	70 °C	954:958	arg1	to					951:952	to	951:952	to	951:952	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	5	17	with	films	864:868	arg1	structures					885:894	permeable structures	875:894	permeable structures	875:894	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	0	18	theme	films	23:27	arg1	properties					9:18	Physical properties	0:18	Physical properties of films	0:27	Physical properties of films based on pectin and babassu coconut mesocarp.					
30822472	1	19	theme	coconut	229:235	arg1	mesocarp					237:244	babassu coconut mesocarp	221:244	babassu coconut mesocarp (BCM)	221:250	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	1	19	theme	coconut	229:235	arg1	BCM					247:249	BCM	247:249	BCM	247:249	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	5	20	dep	temperatures	934:945	arg1	70 °C					954:958	70 °C	954:958	70 °C	954:958	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	2	21	theme	different	367:375	arg1	proportions					377:387	different proportions	367:387	different proportions of the materials	367:404	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	3	22	theme	barrier	696:702	arg1	properties					704:713	barrier properties	696:713	barrier properties	696:713	The results showed a direct relationship between the composition of the film and each physical property, providing films with unique mechanical characteristics and barrier properties.					
30822472	5	23	theme	films	983:987	arg1	confection					969:978	the confection	965:978	the confection of films	965:987	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	3	24	theme	film	604:607	arg1	composition					585:595	the composition	581:595	the composition of the film	581:607	The results showed a direct relationship between the composition of the film and each physical property, providing films with unique mechanical characteristics and barrier properties.					
30822472	3	24	theme	film	604:607	arg1	property					627:634	each physical property	613:634	each physical property	613:634	The results showed a direct relationship between the composition of the film and each physical property, providing films with unique mechanical characteristics and barrier properties.					
30822472	2	25	theme	rotational	339:348	arg1	design					350:355	a central compound rotational design	320:355	a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability	320:529	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	2	26	theme	central	322:328	arg1	design					350:355	a central compound rotational design	320:355	a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability	320:529	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	0	27	dep	pectin	38:43	arg1	mesocarp					65:72	coconut mesocarp	57:72	coconut mesocarp	57:72	Physical properties of films based on pectin and babassu coconut mesocarp.					
30822472	1	28	theme	films	185:189	arg1	structure					172:180	structure	172:180	structure	172:180	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	1	28	theme	films	185:189	arg1	properties					132:141	the physical properties	119:141	the physical properties	119:141	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	1	28	theme	films	185:189	arg1	sorption					150:157	water sorption	144:157	water sorption	144:157	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	1	29	theme	research	97:104	arg1	objective					79:87	The objective	75:87	The objective of this research	75:104	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	2	30	theme	materials	396:404	arg1	proportions					377:387	different proportions	367:387	different proportions of the materials	367:404	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	4	31	theme	G	834:834	arg1	content					836:842	the G content	830:842	the G content	830:842	In the second phase, the films were reformulated, maintaining the proportions of CP and BCM constant and altering the G content.					
30822472	3	32	theme	physical	618:625	arg1	property					627:634	each physical property	613:634	each physical property	613:634	The results showed a direct relationship between the composition of the film and each physical property, providing films with unique mechanical characteristics and barrier properties.					
30822472	2	33	dep	design	350:355	arg1	evaluating					410:419	evaluating	410:419	evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability	410:529	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	2	33	dep	design	350:355	arg1	combining					357:365	combining	357:365	combining different proportions of the materials	357:404	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	2	34	theme	vapor	512:516	arg1	permeability					518:529	water vapor permeability	506:529	water vapor permeability	506:529	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	5	35	theme	high	922:925	arg1	temperatures					934:945	high drying temperatures	922:945	high drying temperatures (up to 70 °C)	922:959	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	2	36	theme	water	506:510	arg1	permeability					518:529	water vapor permeability	506:529	water vapor permeability	506:529	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	5	37	theme	drying	927:932	arg1	temperatures					934:945	high drying temperatures	922:945	high drying temperatures (up to 70 °C)	922:959	The results showed films with permeable structures, thus favoring the use of high drying temperatures (up to 70 °C) for the confection of films, without altering their physical and structural properties.					
30822472	4	38	from	reformulated	752:763	arg1	phase					730:734	the second phase	719:734	the second phase	719:734	In the second phase, the films were reformulated, maintaining the proportions of CP and BCM constant and altering the G content.					
30822472	0	39	theme	coconut	57:63	arg1	mesocarp					65:72	coconut mesocarp	57:72	coconut mesocarp	57:72	Physical properties of films based on pectin and babassu coconut mesocarp.					
30822472	2	40	theme	compound	330:337	arg1	design					350:355	a central compound rotational design	320:355	a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability	320:529	Seventeen formulations were prepared according to a central compound rotational design combining different proportions of the materials and evaluating the films produced according to their moisture contents, solubility, thicknesses and water vapor permeability.					
30822472	3	41	theme	direct	553:558	arg1	relationship					560:571	a direct relationship	551:571	a direct relationship between the composition of the film and each physical property	551:634	The results showed a direct relationship between the composition of the film and each physical property, providing films with unique mechanical characteristics and barrier properties.					
30822472	1	42	theme	citrus	201:206	arg1	CP					216:217	CP	216:217	CP	216:217	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	1	42	theme	citrus	201:206	arg1	pectin					208:213	citrus pectin	201:213	citrus pectin (CP)	201:218	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30822472	1	43	theme	physical	123:130	arg1	properties					132:141	the physical properties	119:141	the physical properties	119:141	The objective of this research was to study the physical properties, water sorption, thermal and structure of films made from citrus pectin (CP), babassu coconut mesocarp (BCM) and glycerol (G).					
30600049	0	0	theme	term	86:89	arg1	applications					115:126	long term excellent antibacterial applications	81:126	long term excellent antibacterial applications	81:126	Cellulose acetate nanofibers embedded with AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications.					
30600049	5	1	theme	composite	838:846	arg1	nanofibers					848:857	CA/TiO2/AgNP composite nanofibers	825:857	CA/TiO2/AgNP composite nanofibers	825:857	Subsequently, the TiO2/AgNP nanocomposites were added into CA solution and electrospun to fabricate CA/TiO2/AgNP composite nanofibers.					
30600049	1	2	theme	effective	162:170	arg1	agents					186:191	effective antimicrobial agents	162:191	effective antimicrobial agents	162:191	Silver nanoparticles (AgNPs) are effective antimicrobial agents however excess release of silver causes argyria and argyrosis.					
30600049	1	2	theme	effective	162:170	arg1	nanoparticles					136:148	Silver nanoparticles	129:148	Silver nanoparticles (AgNPs)	129:156	Silver nanoparticles (AgNPs) are effective antimicrobial agents however excess release of silver causes argyria and argyrosis.					
30600049	2	3	from	immobilization	311:324	arg1	substrates					368:377	several organic and inorganic substrates	338:377	several organic and inorganic substrates	338:377	An strategy to avoid these detrimental side effects is immobilization of AgNPs on several organic and inorganic substrates.					
30600049	0	4	theme	long	81:84	arg1	term					86:89	long term	81:89	long term excellent antibacterial applications	81:126	Cellulose acetate nanofibers embedded with AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications.					
30600049	1	5	theme	antimicrobial	172:184	arg1	agents					186:191	effective antimicrobial agents	162:191	effective antimicrobial agents	162:191	Silver nanoparticles (AgNPs) are effective antimicrobial agents however excess release of silver causes argyria and argyrosis.					
30600049	1	5	theme	antimicrobial	172:184	arg1	nanoparticles					136:148	Silver nanoparticles	129:148	Silver nanoparticles (AgNPs)	129:156	Silver nanoparticles (AgNPs) are effective antimicrobial agents however excess release of silver causes argyria and argyrosis.					
30600049	0	6	theme	antibacterial	101:113	arg1	applications					115:126	long term excellent antibacterial applications	81:126	long term excellent antibacterial applications	81:126	Cellulose acetate nanofibers embedded with AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications.					
30600049	9	7	theme	substantial	1248:1258	arg1	inhibition					1277:1286	substantial bacterial growth inhibition	1248:1286	substantial bacterial growth inhibition for 72 h	1248:1295	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	0	8	theme	excellent	91:99	arg1	applications					115:126	long term excellent antibacterial applications	81:126	long term excellent antibacterial applications	81:126	Cellulose acetate nanofibers embedded with AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications.					
30600049	8	9	theme	SEM	1057:1059	arg1	images					1061:1066	SEM images	1057:1066	SEM images	1057:1066	SEM images indicated regular morphology of the nanofibers.					
30600049	7	10	theme	CA	994:995	arg1	nanofibers					997:1006	CA nanofibers	994:1006	CA nanofibers	994:1006	Synthesis of TiO2/AgNP and its loading into CA nanofibers was confirmed by XRD, XPS, TEM and EDX analysis.					
30600049	4	11	theme	TiO2	631:634	arg1	nanoparticles					636:648	TiO2 nanoparticles	631:648	TiO2 nanoparticles	631:648	The TiO2/AgNP nanocomposite particles were prepared by coating TiO2 nanoparticles with polydopamine hydrochloride followed by a treatment in AgNO3 solution.					
30600049	1	12	theme	Silver	129:134	arg1	agents					186:191	effective antimicrobial agents	162:191	effective antimicrobial agents	162:191	Silver nanoparticles (AgNPs) are effective antimicrobial agents however excess release of silver causes argyria and argyrosis.					
30600049	1	12	theme	Silver	129:134	arg1	AgNPs					151:155	AgNPs	151:155	AgNPs	151:155	Silver nanoparticles (AgNPs) are effective antimicrobial agents however excess release of silver causes argyria and argyrosis.					
30600049	1	12	theme	Silver	129:134	arg1	nanoparticles					136:148	Silver nanoparticles	129:148	Silver nanoparticles (AgNPs)	129:156	Silver nanoparticles (AgNPs) are effective antimicrobial agents however excess release of silver causes argyria and argyrosis.					
30600049	8	13	theme	regular	1078:1084	arg1	morphology					1086:1095	regular morphology	1078:1095	regular morphology of the nanofibers	1078:1113	SEM images indicated regular morphology of the nanofibers.					
30600049	9	14	theme	bacterial	1260:1268	arg1	inhibition					1277:1286	substantial bacterial growth inhibition	1248:1286	substantial bacterial growth inhibition for 72 h	1248:1295	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	2	15	theme	side	295:298	arg1	effects					300:306	these detrimental side effects	277:306	these detrimental side effects	277:306	An strategy to avoid these detrimental side effects is immobilization of AgNPs on several organic and inorganic substrates.					
30600049	3	16	theme	environmentally	451:465	arg1	process					473:479	an environmentally green process	448:479	an environmentally green process	448:479	Herein, we propose immobilization of AgNPs on TiO2 nanoparticles by an environmentally green process subsequently incorporating the TiO2/AgNP into cellulose acetate (CA) nanofiber matrix.					
30600049	3	17	from	immobilization	399:412	arg1	nanoparticles					431:443	TiO2 nanoparticles	426:443	TiO2 nanoparticles	426:443	Herein, we propose immobilization of AgNPs on TiO2 nanoparticles by an environmentally green process subsequently incorporating the TiO2/AgNP into cellulose acetate (CA) nanofiber matrix.					
30600049	7	18	theme	loading	981:987	arg1	Synthesis					950:958	Synthesis	950:958	Synthesis of TiO2/AgNP and its loading into CA nanofibers	950:1006	Synthesis of TiO2/AgNP and its loading into CA nanofibers was confirmed by XRD, XPS, TEM and EDX analysis.					
30600049	2	19	theme	detrimental	283:293	arg1	effects					300:306	these detrimental side effects	277:306	these detrimental side effects	277:306	An strategy to avoid these detrimental side effects is immobilization of AgNPs on several organic and inorganic substrates.					
30600049	3	20	theme	cellulose	527:535	arg1	matrix					560:565	cellulose acetate (CA) nanofiber matrix	527:565	cellulose acetate (CA) nanofiber matrix	527:565	Herein, we propose immobilization of AgNPs on TiO2 nanoparticles by an environmentally green process subsequently incorporating the TiO2/AgNP into cellulose acetate (CA) nanofiber matrix.					
30600049	2	21	dep	strategy	259:266	arg1	avoid					271:275	avoid	271:275	to avoid these detrimental side effects	268:306	An strategy to avoid these detrimental side effects is immobilization of AgNPs on several organic and inorganic substrates.					
30600049	4	22	theme	AgNO3	709:713	arg1	solution					715:722	AgNO3 solution	709:722	AgNO3 solution	709:722	The TiO2/AgNP nanocomposite particles were prepared by coating TiO2 nanoparticles with polydopamine hydrochloride followed by a treatment in AgNO3 solution.					
30600049	6	23	theme	antibacterial	928:940	arg1	assays					942:947	antibacterial assays	928:947	antibacterial assays	928:947	The samples were characterized by XRD, TEM, XPS, SEM, EDX, FTIR and antibacterial assays.					
30600049	1	24	theme	excess	201:206	arg1	release					208:214	excess release	201:214	excess release of silver	201:224	Silver nanoparticles (AgNPs) are effective antimicrobial agents however excess release of silver causes argyria and argyrosis.					
30600049	0	25	theme	Cellulose	0:8	arg1	acetate					10:16	Cellulose acetate	0:16	Cellulose acetate	0:16	Cellulose acetate nanofibers embedded with AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications.					
30600049	9	26	contain	having	1191:1196	arg1	nanofibers					1180:1189	CA/TiO2/AgNP composite nanofibers	1157:1189	CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h	1157:1295	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	9	26	contain	having	1191:1196	arg2	performances					1222:1233	excellent antibacterial performances	1198:1233	excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h	1198:1295	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	2	27	theme	AgNPs	329:333	arg1	strategy					259:266	An strategy	256:266	An strategy to avoid these detrimental side effects	256:306	An strategy to avoid these detrimental side effects is immobilization of AgNPs on several organic and inorganic substrates.					
30600049	2	27	theme	AgNPs	329:333	arg1	immobilization					311:324	immobilization	311:324	immobilization of AgNPs on several organic and inorganic substrates	311:377	An strategy to avoid these detrimental side effects is immobilization of AgNPs on several organic and inorganic substrates.					
30600049	9	28	theme	antibacterial	1120:1132	arg1	results					1139:1145	The antibacterial test results	1116:1145	The antibacterial test results	1116:1145	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	4	29	theme	nanocomposite	582:594	arg1	particles					596:604	The TiO2/AgNP nanocomposite particles	568:604	The TiO2/AgNP nanocomposite particles	568:604	The TiO2/AgNP nanocomposite particles were prepared by coating TiO2 nanoparticles with polydopamine hydrochloride followed by a treatment in AgNO3 solution.					
30600049	8	30	theme	nanofibers	1104:1113	arg1	morphology					1086:1095	regular morphology	1078:1095	regular morphology of the nanofibers	1078:1113	SEM images indicated regular morphology of the nanofibers.					
30600049	4	31	from	treatment	696:704	arg1	solution					715:722	AgNO3 solution	709:722	AgNO3 solution	709:722	The TiO2/AgNP nanocomposite particles were prepared by coating TiO2 nanoparticles with polydopamine hydrochloride followed by a treatment in AgNO3 solution.					
30600049	1	32	theme	silver	219:224	arg1	release					208:214	excess release	201:214	excess release of silver	201:224	Silver nanoparticles (AgNPs) are effective antimicrobial agents however excess release of silver causes argyria and argyrosis.					
30600049	3	33	theme	TiO2	426:429	arg1	nanoparticles					431:443	TiO2 nanoparticles	426:443	TiO2 nanoparticles	426:443	Herein, we propose immobilization of AgNPs on TiO2 nanoparticles by an environmentally green process subsequently incorporating the TiO2/AgNP into cellulose acetate (CA) nanofiber matrix.					
30600049	5	34	theme	CA	784:785	arg1	solution					787:794	CA solution	784:794	CA solution	784:794	Subsequently, the TiO2/AgNP nanocomposites were added into CA solution and electrospun to fabricate CA/TiO2/AgNP composite nanofibers.					
30600049	0	35	dep	anchored	49:56	arg1	AgNPs					43:47	AgNPs	43:47	AgNPs	43:47	Cellulose acetate nanofibers embedded with AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications.					
30600049	0	36	with	embedded	29:36	arg1	nanoparticles					63:75	AgNPs anchored TiO2 nanoparticles	43:75	AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications	43:126	Cellulose acetate nanofibers embedded with AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications.					
30600049	0	37	theme	TiO2	58:61	arg1	nanoparticles					63:75	AgNPs anchored TiO2 nanoparticles	43:75	AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications	43:126	Cellulose acetate nanofibers embedded with AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications.					
30600049	5	38	theme	CA/TiO2/AgNP	825:836	arg1	nanofibers					848:857	CA/TiO2/AgNP composite nanofibers	825:857	CA/TiO2/AgNP composite nanofibers	825:857	Subsequently, the TiO2/AgNP nanocomposites were added into CA solution and electrospun to fabricate CA/TiO2/AgNP composite nanofibers.					
30600049	7	39	theme	EDX	1043:1045	arg1	analysis					1047:1054	EDX analysis	1043:1054	EDX analysis	1043:1054	Synthesis of TiO2/AgNP and its loading into CA nanofibers was confirmed by XRD, XPS, TEM and EDX analysis.					
30600049	2	40	theme	inorganic	358:366	arg1	substrates					368:377	several organic and inorganic substrates	338:377	several organic and inorganic substrates	338:377	An strategy to avoid these detrimental side effects is immobilization of AgNPs on several organic and inorganic substrates.					
30600049	0	41	theme	anchored	49:56	arg1	nanoparticles					63:75	AgNPs anchored TiO2 nanoparticles	43:75	AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications	43:126	Cellulose acetate nanofibers embedded with AgNPs anchored TiO2 nanoparticles for long term excellent antibacterial applications.					
30600049	4	42	theme	TiO2/AgNP	572:580	arg1	particles					596:604	The TiO2/AgNP nanocomposite particles	568:604	The TiO2/AgNP nanocomposite particles	568:604	The TiO2/AgNP nanocomposite particles were prepared by coating TiO2 nanoparticles with polydopamine hydrochloride followed by a treatment in AgNO3 solution.					
30600049	9	43	theme	test	1134:1137	arg1	results					1139:1145	The antibacterial test results	1116:1145	The antibacterial test results	1116:1145	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	5	44	theme	TiO2/AgNP	743:751	arg1	nanocomposites					753:766	the TiO2/AgNP nanocomposites	739:766	the TiO2/AgNP nanocomposites	739:766	Subsequently, the TiO2/AgNP nanocomposites were added into CA solution and electrospun to fabricate CA/TiO2/AgNP composite nanofibers.					
30600049	9	45	theme	growth	1270:1275	arg1	inhibition					1277:1286	substantial bacterial growth inhibition	1248:1286	substantial bacterial growth inhibition for 72 h	1248:1295	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	9	46	theme	composite	1170:1178	arg1	nanofibers					1180:1189	CA/TiO2/AgNP composite nanofibers	1157:1189	CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h	1157:1295	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	2	47	theme	organic	346:352	arg1	substrates					368:377	several organic and inorganic substrates	338:377	several organic and inorganic substrates	338:377	An strategy to avoid these detrimental side effects is immobilization of AgNPs on several organic and inorganic substrates.					
30600049	7	48	theme	TiO2/AgNP	963:971	arg1	Synthesis					950:958	Synthesis	950:958	Synthesis of TiO2/AgNP and its loading into CA nanofibers	950:1006	Synthesis of TiO2/AgNP and its loading into CA nanofibers was confirmed by XRD, XPS, TEM and EDX analysis.					
30600049	4	49	theme	polydopamine	655:666	arg1	hydrochloride					668:680	polydopamine hydrochloride	655:680	polydopamine hydrochloride followed by a treatment in AgNO3 solution	655:722	The TiO2/AgNP nanocomposite particles were prepared by coating TiO2 nanoparticles with polydopamine hydrochloride followed by a treatment in AgNO3 solution.					
30600049	9	50	theme	CA/TiO2/AgNP	1157:1168	arg1	nanofibers					1180:1189	CA/TiO2/AgNP composite nanofibers	1157:1189	CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h	1157:1295	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	9	51	theme	excellent	1198:1206	arg1	performances					1222:1233	excellent antibacterial performances	1198:1233	excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h	1198:1295	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	2	52	theme	several	338:344	arg1	substrates					368:377	several organic and inorganic substrates	338:377	several organic and inorganic substrates	338:377	An strategy to avoid these detrimental side effects is immobilization of AgNPs on several organic and inorganic substrates.					
30600049	9	53	theme	antibacterial	1208:1220	arg1	performances					1222:1233	excellent antibacterial performances	1198:1233	excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h	1198:1295	The antibacterial test results confirmed CA/TiO2/AgNP composite nanofibers having excellent antibacterial performances for 36 h and substantial bacterial growth inhibition for 72 h.					
30600049	3	54	theme	green	467:471	arg1	process					473:479	an environmentally green process	448:479	an environmentally green process	448:479	Herein, we propose immobilization of AgNPs on TiO2 nanoparticles by an environmentally green process subsequently incorporating the TiO2/AgNP into cellulose acetate (CA) nanofiber matrix.					
30600049	3	55	theme	AgNPs	417:421	arg1	immobilization					399:412	immobilization	399:412	immobilization of AgNPs on TiO2 nanoparticles	399:443	Herein, we propose immobilization of AgNPs on TiO2 nanoparticles by an environmentally green process subsequently incorporating the TiO2/AgNP into cellulose acetate (CA) nanofiber matrix.					
30600049	3	56	theme	nanofiber	550:558	arg1	matrix					560:565	cellulose acetate (CA) nanofiber matrix	527:565	cellulose acetate (CA) nanofiber matrix	527:565	Herein, we propose immobilization of AgNPs on TiO2 nanoparticles by an environmentally green process subsequently incorporating the TiO2/AgNP into cellulose acetate (CA) nanofiber matrix.					
31690047	8	0	theme	development	1400:1410	arg1	terms					1367:1371	terms	1367:1371	terms of protoplast-derived cell development	1367:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	1	from	walls	1467:1471	arg1	cultures					1508:1515	the PSK-treated cultures	1492:1515	the PSK-treated cultures	1492:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	1	from	walls	1467:1471	arg1	control					1480:1486	control	1480:1486	control	1480:1486	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	2	theme	chemical	1434:1441	arg1	composition					1443:1453	the chemical composition	1430:1453	the chemical composition of the cell walls in the control and the PSK-treated cultures	1430:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	7	3	used	used	1278:1281	arg2	antibodies					1149:1158	Several antibodies	1141:1158	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12)	1141:1271	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	8	4	from	control	1480:1486	arg1	composition					1443:1453	the chemical composition	1430:1453	the chemical composition of the cell walls in the control and the PSK-treated cultures	1430:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	1	5	theme	intercellular	209:221	arg1	Phytosulfokine-α					118:133	Phytosulfokine-α	118:133	Phytosulfokine-α (PSK)	118:139	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	1	5	theme	intercellular	209:221	arg1	molecule					233:240	a promising intercellular signaling molecule	197:240	a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation	197:297	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	0	6	theme	Daucus	74:79	arg1	Cells					65:69	Protoplast-Derived Cells	46:69	Protoplast-Derived Cells of Daucus	46:79	Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus is Affected by Phytosulfokine (PSK).					
31690047	5	7	theme	PSK	1008:1010	arg1	presence					996:1003	the presence	992:1003	the presence of PSK during the protoplast culture	992:1040	Even less is known about the correlation between wall regeneration and the presence of PSK during the protoplast culture.					
31690047	5	7	theme	PSK	1008:1010	arg1	regeneration					975:986	wall regeneration	970:986	wall regeneration	970:986	Even less is known about the correlation between wall regeneration and the presence of PSK during the protoplast culture.					
31690047	1	8	theme	signaling	223:231	arg1	Phytosulfokine-α					118:133	Phytosulfokine-α	118:133	Phytosulfokine-α (PSK)	118:139	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	1	8	theme	signaling	223:231	arg1	molecule					233:240	a promising intercellular signaling molecule	197:240	a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation	197:297	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	4	9	theme	wall	887:890	arg1	reconstruction					865:878	the reconstruction	861:878	the reconstruction of the wall in protoplast-derived cells	861:918	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	0	10	from	Composition	0:10	arg1	Cells					65:69	Protoplast-Derived Cells	46:69	Protoplast-Derived Cells of Daucus	46:79	Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus is Affected by Phytosulfokine (PSK).					
31690047	3	11	theme	somatic	519:525	arg1	embryogenesis					527:539	somatic embryogenesis	519:539	somatic embryogenesis	519:539	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	8	12	theme	obtained	1288:1295	arg1	results					1297:1303	The obtained results	1284:1303	The obtained results	1284:1303	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	4	13	from	wall	887:890	arg1	cells					914:918	protoplast-derived cells	895:918	protoplast-derived cells	895:918	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	4	14	from	cells	914:918	arg1	reconstruction					865:878	the reconstruction	861:878	the reconstruction of the wall in protoplast-derived cells	861:918	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	3	15	from	increase	493:500	arg1	efficiency					505:514	efficiency	505:514	efficiency of somatic embryogenesis	505:539	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	3	16	theme	embryogenesis	527:539	arg1	efficiency					505:514	efficiency	505:514	efficiency of somatic embryogenesis	505:539	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	8	17	from	response	1324:1331	arg1	terms					1367:1371	terms	1367:1371	terms of protoplast-derived cell development	1367:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	17	from	response	1324:1331	arg1	composition					1443:1453	the chemical composition	1430:1453	the chemical composition of the cell walls in the control and the PSK-treated cultures	1430:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	4	18	theme	protoplast-derived	895:912	arg1	cells					914:918	protoplast-derived cells	895:918	protoplast-derived cells	895:918	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	1	19	theme	plant	153:157	arg1	Phytosulfokine-α					118:133	Phytosulfokine-α	118:133	Phytosulfokine-α (PSK)	118:139	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	1	19	theme	plant	153:157	arg1	factor					166:171	a peptidyl plant growth factor	142:171	a peptidyl plant growth factor	142:171	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	7	20	theme	Several	1141:1147	arg1	antibodies					1149:1158	Several antibodies	1141:1158	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12)	1141:1271	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	8	21	from	terms	1367:1371	arg1	diversity					1417:1425	diversity	1417:1425	diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures	1417:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	21	from	terms	1367:1371	arg1	response					1324:1331	a diverse response	1314:1331	a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development	1314:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	4	22	theme	in	735:736	arg1	cultures					744:751	in vitro cultures	735:751	in vitro cultures	735:751	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	8	23	from	composition	1443:1453	arg1	cultures					1508:1515	the PSK-treated cultures	1492:1515	the PSK-treated cultures	1492:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	23	from	composition	1443:1453	arg1	control					1480:1486	control	1480:1486	control	1480:1486	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	1	24	theme	peptidyl	144:151	arg1	Phytosulfokine-α					118:133	Phytosulfokine-α	118:133	Phytosulfokine-α (PSK)	118:139	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	1	24	theme	peptidyl	144:151	arg1	factor					166:171	a peptidyl plant growth factor	142:171	a peptidyl plant growth factor	142:171	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	2	25	theme	mass	440:443	arg1	formation					445:453	proembryogenic mass formation	425:453	proembryogenic mass formation	425:453	It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation.					
31690047	7	26	theme	wall	1177:1180	arg1	components					1182:1191	wall components	1177:1191	wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12)	1177:1271	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	7	27	dep	anti-pectin	1194:1204	arg1	anti-extensin					1251:1263	anti-extensin	1251:1263	anti-extensin	1251:1263	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	7	27	dep	anti-pectin	1194:1204	arg1	LM19					1207:1210	LM19	1207:1210	LM19	1207:1210	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	7	27	dep	anti-pectin	1194:1204	arg1	JIM4					1229:1232	JIM4	1229:1232	JIM4	1229:1232	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	7	27	dep	anti-pectin	1194:1204	arg1	JIM8					1235:1238	JIM8	1235:1238	JIM8	1235:1238	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	7	27	dep	anti-pectin	1194:1204	arg1	JIM12					1266:1270	JIM12	1266:1270	JIM12	1266:1270	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	7	27	dep	anti-pectin	1194:1204	arg1	JIM13					1241:1245	JIM13	1241:1245	JIM13	1241:1245	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	2	28	theme	protoplast	364:373	arg1	cultures					375:382	protoplast cultures	364:382	protoplast cultures of several species leading to callus and proembryogenic mass formation	364:453	It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation.					
31690047	2	29	theme	proembryogenic	425:438	arg1	formation					445:453	proembryogenic mass formation	425:453	proembryogenic mass formation	425:453	It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation.					
31690047	4	30	theme	arabinogalactan	793:807	arg1	AGP					818:820	AGP	818:820	AGP	818:820	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	4	30	theme	arabinogalactan	793:807	arg1	protein					809:815	arabinogalactan protein	793:815	arabinogalactan protein (AGP)	793:821	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	1	31	theme	growth	159:164	arg1	Phytosulfokine-α					118:133	Phytosulfokine-α	118:133	Phytosulfokine-α (PSK)	118:139	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	1	31	theme	growth	159:164	arg1	factor					166:171	a peptidyl plant growth factor	142:171	a peptidyl plant growth factor	142:171	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	0	32	theme	Reconstituted	19:31	arg1	Wall					38:41	the Reconstituted Cell Wall	15:41	the Reconstituted Cell Wall	15:41	Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus is Affected by Phytosulfokine (PSK).					
31690047	1	33	theme	cellular	254:261	arg1	proliferation					263:275	cellular proliferation	254:275	cellular proliferation	254:275	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	4	34	theme	model	717:721	arg1	carrot					707:712	the carrot	703:712	the carrot	703:712	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	4	34	theme	model	717:721	arg1	species					723:729	a model species	715:729	a model species for in vitro cultures	715:751	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	8	35	from	diversity	1417:1425	arg1	terms					1367:1371	terms	1367:1371	terms of protoplast-derived cell development	1367:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	35	from	diversity	1417:1425	arg1	composition					1443:1453	the chemical composition	1430:1453	the chemical composition of the cell walls in the control and the PSK-treated cultures	1430:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	36	theme	walls	1467:1471	arg1	composition					1443:1453	the chemical composition	1430:1453	the chemical composition of the cell walls in the control and the PSK-treated cultures	1430:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	37	from	cultures	1508:1515	arg1	composition					1443:1453	the chemical composition	1430:1453	the chemical composition of the cell walls in the control and the PSK-treated cultures	1430:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	3	38	theme	cell	625:628	arg1	walls					630:634	the cell walls	621:634	the cell walls	621:634	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	6	39	theme	cultivated	1076:1085	arg1	carrot					1087:1092	the cultivated carrot	1072:1092	the cultivated carrot	1072:1092	Three Daucus taxa, including the cultivated carrot, were analyzed during protoplast regeneration.					
31690047	8	40	link	protoplast-derived	1376:1393	arg1	development					1400:1410	protoplast-derived cell development	1376:1410	protoplast-derived cell development	1376:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	0	41	theme	Wall	38:41	arg1	Composition					0:10	Composition	0:10	Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus	0:79	Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus is Affected by Phytosulfokine (PSK).					
31690047	8	42	theme	cell	1462:1465	arg1	walls					1467:1471	the cell walls	1458:1471	the cell walls in the control and the PSK-treated cultures	1458:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	7	43	dep	components	1182:1191	arg1	anti-pectin					1194:1204	anti-pectin	1194:1204	anti-pectin	1194:1204	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	7	43	dep	components	1182:1191	arg1	anti-AGP					1219:1226	anti-AGP	1219:1226	anti-AGP	1219:1226	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	7	43	dep	components	1182:1191	arg1	LM20					1213:1216	LM20	1213:1216	LM20	1213:1216	Several antibodies directed against wall components (anti-pectin: LM19, LM20, anti-AGP: JIM4, JIM8, JIM13 and anti-extensin: JIM12) were used.					
31690047	3	44	theme	walls	630:634	arg1	components					607:616	selected chemical components	589:616	selected chemical components of the cell walls	589:634	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	2	45	theme	species	395:401	arg1	cultures					375:382	protoplast cultures	364:382	protoplast cultures of several species leading to callus and proembryogenic mass formation	364:453	It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation.					
31690047	0	46	theme	Cell	33:36	arg1	Wall					38:41	the Reconstituted Cell Wall	15:41	the Reconstituted Cell Wall	15:41	Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus is Affected by Phytosulfokine (PSK).					
31690047	2	47	theme	several	387:393	arg1	species					395:401	several species	387:401	several species leading to callus and proembryogenic mass formation	387:453	It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation.					
31690047	0	48	theme	Protoplast-Derived	46:63	arg1	Cells					65:69	Protoplast-Derived Cells	46:69	Protoplast-Derived Cells of Daucus	46:79	Composition of the Reconstituted Cell Wall in Protoplast-Derived Cells of Daucus is Affected by Phytosulfokine (PSK).					
31690047	4	49	link	protoplast-derived	895:912	arg1	cells					914:918	protoplast-derived cells	895:918	protoplast-derived cells	895:918	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	5	50	theme	protoplast	1023:1032	arg1	culture					1034:1040	the protoplast culture	1019:1040	the protoplast culture	1019:1040	Even less is known about the correlation between wall regeneration and the presence of PSK during the protoplast culture.					
31690047	5	51	theme	wall	970:973	arg1	regeneration					975:986	wall regeneration	970:986	wall regeneration	970:986	Even less is known about the correlation between wall regeneration and the presence of PSK during the protoplast culture.					
31690047	6	52	theme	protoplast	1116:1125	arg1	regeneration					1127:1138	protoplast regeneration	1116:1138	protoplast regeneration	1116:1138	Three Daucus taxa, including the cultivated carrot, were analyzed during protoplast regeneration.					
31690047	4	53	theme	extensin	827:834	arg1	epitopes					836:843	extensin epitopes	827:843	extensin epitopes	827:843	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	3	54	theme	protoplast	647:656	arg1	process					671:677	the protoplast regeneration process	643:677	the protoplast regeneration process	643:677	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	8	55	theme	diverse	1316:1322	arg1	response					1324:1331	a diverse response	1314:1331	a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development	1314:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	56	theme	taxa	1352:1355	arg1	diversity					1417:1425	diversity	1417:1425	diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures	1417:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	56	theme	taxa	1352:1355	arg1	response					1324:1331	a diverse response	1314:1331	a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development	1314:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	57	theme	used	1340:1343	arg1	taxa					1352:1355	the used Daucus taxa	1336:1355	the used Daucus taxa to PSK in terms of protoplast-derived cell development	1336:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	3	58	theme	regeneration	658:669	arg1	process					671:677	the protoplast regeneration process	643:677	the protoplast regeneration process	643:677	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	3	59	theme	chemical	598:605	arg1	components					607:616	selected chemical components	589:616	selected chemical components of the cell walls	589:634	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	8	60	theme	PSK-treated	1496:1506	arg1	cultures					1508:1515	the PSK-treated cultures	1492:1515	the PSK-treated cultures	1492:1515	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	61	theme	cell	1395:1398	arg1	development					1400:1410	protoplast-derived cell development	1376:1410	protoplast-derived cell development	1376:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	3	62	theme	components	607:616	arg1	distribution					573:584	the distribution	569:584	the distribution of selected chemical components of the cell walls	569:634	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	4	63	from	reconstruction	865:878	arg1	cells					914:918	protoplast-derived cells	895:918	protoplast-derived cells	895:918	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	4	64	dep	in	735:736	arg1	vitro					738:742	vitro	738:742	vitro	738:742	So far, especially for the carrot, a model species for in vitro cultures, it has not been specified what pectic, arabinogalactan protein (AGP) and extensin epitopes are involved in the reconstruction of the wall in protoplast-derived cells.					
31690047	8	65	theme	protoplast-derived	1376:1393	arg1	development					1400:1410	protoplast-derived cell development	1376:1410	protoplast-derived cell development	1376:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	8	66	theme	Daucus	1345:1350	arg1	taxa					1352:1355	the used Daucus taxa	1336:1355	the used Daucus taxa to PSK in terms of protoplast-derived cell development	1336:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	6	67	theme	Daucus	1049:1054	arg1	carrot					1087:1092	the cultivated carrot	1072:1092	the cultivated carrot	1072:1092	Three Daucus taxa, including the cultivated carrot, were analyzed during protoplast regeneration.					
31690047	6	67	theme	Daucus	1049:1054	arg1	taxa					1056:1059	Three Daucus taxa	1043:1059	Three Daucus taxa	1043:1059	Three Daucus taxa, including the cultivated carrot, were analyzed during protoplast regeneration.					
31690047	8	68	from	taxa	1352:1355	arg1	terms					1367:1371	terms	1367:1371	terms of protoplast-derived cell development	1367:1410	The obtained results indicate a diverse response of the used Daucus taxa to PSK in terms of protoplast-derived cell development, and diversity in the chemical composition of the cell walls in the control and the PSK-treated cultures.					
31690047	3	69	theme	selected	589:596	arg1	components					607:616	selected chemical components	589:616	selected chemical components of the cell walls	589:634	Since PSK had been shown to cause an increase in efficiency of somatic embryogenesis, it was reasonable to check the distribution of selected chemical components of the cell walls during the protoplast regeneration process.					
31690047	2	70	theme	cell	346:349	arg1	divisions					351:359	cell divisions	346:359	cell divisions	346:359	It was shown that PSK stimulated and enhanced cell divisions in protoplast cultures of several species leading to callus and proembryogenic mass formation.					
31690047	1	71	theme	promising	199:207	arg1	Phytosulfokine-α					118:133	Phytosulfokine-α	118:133	Phytosulfokine-α (PSK)	118:139	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31690047	1	71	theme	promising	199:207	arg1	molecule					233:240	a promising intercellular signaling molecule	197:240	a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation	197:297	Phytosulfokine-α (PSK), a peptidyl plant growth factor, has been recognized as a promising intercellular signaling molecule involved in cellular proliferation and dedifferentiation.					
31145052	6	0	with	perfusion	1035:1043	arg1	solution					1054:1061	any solution	1050:1061	any solution	1050:1061	RESULTS In experiment 1, no significant cellular injury was shown at up to 3 hours of perfusion with any solution.					
31145052	11	1	theme	CONCLUSIONS	1730:1740	arg1	damage					1750:1755	CONCLUSIONS Tubular damage	1730:1755	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis)	1730:1837	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS.					
31145052	9	2	theme	tubular	1489:1495	arg1	damage					1497:1502	tubular damage	1489:1502	tubular damage	1489:1502	In experiment 2, after ischemia-reperfusion injury, no significant differences were found between HTK and BUPS groups regarding tubular damage.					
31145052	10	3	theme	pig	1613:1615	arg1	group					1629:1633	pig reperfusion group	1613:1633	pig reperfusion group	1613:1633	Although no significant differences were shown regarding tubular cytoskeletal rearrangment and apoptosis in pig reperfusion group with BUPS versus HTK, significant differences were shown with these solutions in other groups.					
31145052	12	4	theme	cost-effective	1875:1888	arg1	solution					1900:1907	a cost-effective perfusion solution	1873:1907	a cost-effective perfusion solution in transplantation	1873:1926	BUPS can be a cost-effective perfusion solution in transplantation.					
31145052	12	4	theme	cost-effective	1875:1888	arg1	BUPS					1861:1864	BUPS	1861:1864	BUPS	1861:1864	BUPS can be a cost-effective perfusion solution in transplantation.					
31145052	1	5	theme	cold	173:176	arg1	time					187:190	long cold ischemia time	168:190	long cold ischemia time	168:190	OBJECTIVES Organ damage due to long cold ischemia time remains a hurdle in transplantation.					
31145052	2	6	theme	Preservation	302:313	arg1	BUPS					325:328	the new Baskent University Preservation Solution (BUPS)	275:329	the new Baskent University Preservation Solution (BUPS)	275:329	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	0	7	theme	Animal	123:128	arg1	Study					130:134	Preliminary Experimental Animal Study	98:134	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.	0:135	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	10	8	theme	tubular	1562:1568	arg1	rearrangment					1583:1594	tubular cytoskeletal rearrangment	1562:1594	tubular cytoskeletal rearrangment	1562:1594	Although no significant differences were shown regarding tubular cytoskeletal rearrangment and apoptosis in pig reperfusion group with BUPS versus HTK, significant differences were shown with these solutions in other groups.					
31145052	4	9	from	rats	618:621	arg1	kidneys					582:588	kidneys	582:588	kidneys from 50 male Sprague-Dawley rats	582:621	In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation.					
31145052	10	10	theme	significant	1657:1667	arg1	differences					1669:1679	significant differences	1657:1679	significant differences	1657:1679	Although no significant differences were shown regarding tubular cytoskeletal rearrangment and apoptosis in pig reperfusion group with BUPS versus HTK, significant differences were shown with these solutions in other groups.					
31145052	1	11	from	hurdle	202:207	arg1	transplantation					212:226	transplantation	212:226	transplantation	212:226	OBJECTIVES Organ damage due to long cold ischemia time remains a hurdle in transplantation.					
31145052	5	12	theme	ischemia-reperfusion	802:821	arg1	injury					823:828	ischemia-reperfusion injury	802:828	ischemia-reperfusion injury	802:828	In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other.					
31145052	4	13	theme	ischemia	681:688	arg1	injury					690:695	cold ischemia injury	676:695	cold ischemia injury	676:695	In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation.					
31145052	10	14	theme	reperfusion	1617:1627	arg1	group					1629:1633	pig reperfusion group	1613:1633	pig reperfusion group	1613:1633	Although no significant differences were shown regarding tubular cytoskeletal rearrangment and apoptosis in pig reperfusion group with BUPS versus HTK, significant differences were shown with these solutions in other groups.					
31145052	1	15	theme	OBJECTIVES	137:146	arg1	damage					154:159	OBJECTIVES Organ damage	137:159	OBJECTIVES Organ damage due to long cold ischemia time	137:190	OBJECTIVES Organ damage due to long cold ischemia time remains a hurdle in transplantation.					
31145052	3	16	theme	METHODS	439:445	arg1	composition					452:462	MATERIALS AND METHODS BUPS composition	425:462	MATERIALS AND METHODS BUPS composition	425:462	MATERIALS AND METHODS BUPS composition included electrolytes, raffinose, mannitol, N-acetylcysteine, taurine, adenosine, and ascorbic acid.					
31145052	0	17	theme	Kidney	63:68	arg1	Graft					70:74	Kidney Graft	63:74	Kidney Graft	63:74	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	2	18	theme	preliminary	237:247	arg1	study					256:260	this preliminary animal study	232:260	this preliminary animal study	232:260	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	5	19	theme	renal	843:847	arg1	donors					860:865	renal transplant donors	843:865	renal transplant donors to 10 rats	843:876	In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other.					
31145052	8	20	theme	BUPS	1197:1200	arg1	group					1202:1206	The BUPS group	1193:1206	The BUPS group	1193:1206	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	7	21	theme	lowest	1123:1128	arg1	injury					1130:1135	lowest injury	1123:1135	lowest injury in BUPS and HTK versus UW and control groups (P < .01)	1123:1190	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	7	22	from	injury	1130:1135	arg1	HTK					1149:1151	HTK	1149:1151	HTK	1149:1151	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	7	22	from	injury	1130:1135	arg1	<					1185:1185	P < .01	1183:1189	P < .01	1183:1189	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	7	22	from	injury	1130:1135	arg1	BUPS					1140:1143	BUPS	1140:1143	BUPS	1140:1143	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	7	22	from	injury	1130:1135	arg1	UW					1160:1161	UW	1160:1161	UW	1160:1161	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	8	23	theme	P	1351:1351	arg1	perfusion					1340:1348	48-hour perfusion	1332:1348	48-hour perfusion (P < .01)	1332:1358	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	8	23	theme	P	1351:1351	arg1	<					1353:1353	P < .01	1351:1357	P < .01	1351:1357	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	5	24	theme	transplant	902:911	arg1	donors-recipients					913:929	transplant donors-recipients	902:929	transplant donors-recipients among each other	902:946	In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other.					
31145052	5	24	theme	transplant	902:911	arg1	pigs					884:887	6 pigs	882:887	6 pigs	882:887	In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other.					
31145052	11	25	theme	increased	1818:1826	arg1	disruption					1806:1815	cytoskeletal disruption	1793:1815	cytoskeletal disruption	1793:1815	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS.					
31145052	11	25	theme	increased	1818:1826	arg1	apoptosis					1828:1836	increased apoptosis	1818:1836	increased apoptosis	1818:1836	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS.					
31145052	4	26	theme	time	731:734	arg1	points					736:741	different time points	721:741	different time points for pathologic evaluation	721:767	In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation.					
31145052	5	27	used	used	894:897	arg2	donors-recipients					913:929	transplant donors-recipients	902:929	transplant donors-recipients among each other	902:946	In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other.					
31145052	5	27	used	used	894:897	arg2	pigs					884:887	6 pigs	882:887	6 pigs	882:887	In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other.					
31145052	0	28	theme	Preliminary	98:108	arg1	Study					130:134	Preliminary Experimental Animal Study	98:134	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.	0:135	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	7	29	dep	6-	1067:1068	arg1	to					1070:1071	to	1070:1071	to	1070:1071	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	2	30	theme	UW	365:366	arg1	solutions					414:422	Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions	354:422	Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions	354:422	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	7	31	theme	tubular	1092:1098	arg1	injury					1100:1105	tubular injury	1092:1105	tubular injury	1092:1105	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	9	32	located	found	1445:1449	arg1	experiment					1364:1373	experiment 2	1364:1375	experiment 2	1364:1375	In experiment 2, after ischemia-reperfusion injury, no significant differences were found between HTK and BUPS groups regarding tubular damage.					
31145052	9	32	located	found	1445:1449	arg2	differences					1428:1438	no significant differences	1413:1438	no significant differences	1413:1438	In experiment 2, after ischemia-reperfusion injury, no significant differences were found between HTK and BUPS groups regarding tubular damage.					
31145052	8	33	theme	apoptosis	1247:1255	arg1	rearrangement					1274:1286	cytoskeletal rearrangement	1261:1286	cytoskeletal rearrangement	1261:1286	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	8	33	theme	apoptosis	1247:1255	arg1	degree					1229:1234	more moderate degree	1215:1234	more moderate degree of tubular apoptosis	1215:1255	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	6	34	dep	3	1024:1024	arg1	to					1021:1022	to	1021:1022	to	1021:1022	RESULTS In experiment 1, no significant cellular injury was shown at up to 3 hours of perfusion with any solution.					
31145052	10	35	theme	significant	1517:1527	arg1	differences					1529:1539	no significant differences	1514:1539	no significant differences	1514:1539	Although no significant differences were shown regarding tubular cytoskeletal rearrangment and apoptosis in pig reperfusion group with BUPS versus HTK, significant differences were shown with these solutions in other groups.					
31145052	0	36	theme	New	14:16	arg1	Solution					50:57	New Baskent University Preservation Solution	14:57	New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia	14:95	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	6	37	theme	cellular	989:996	arg1	injury					998:1003	no significant cellular injury	974:1003	no significant cellular injury	974:1003	RESULTS In experiment 1, no significant cellular injury was shown at up to 3 hours of perfusion with any solution.					
31145052	10	38	theme	other	1716:1720	arg1	groups					1722:1727	other groups	1716:1727	other groups	1716:1727	Although no significant differences were shown regarding tubular cytoskeletal rearrangment and apoptosis in pig reperfusion group with BUPS versus HTK, significant differences were shown with these solutions in other groups.					
31145052	8	39	theme	cytoskeletal	1261:1272	arg1	rearrangement					1274:1286	cytoskeletal rearrangement	1261:1286	cytoskeletal rearrangement	1261:1286	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	11	40	theme	cytoskeletal	1793:1804	arg1	disruption					1806:1815	cytoskeletal disruption	1793:1815	cytoskeletal disruption	1793:1815	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS.					
31145052	11	40	theme	cytoskeletal	1793:1804	arg1	apoptosis					1828:1836	increased apoptosis	1818:1836	increased apoptosis	1818:1836	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS.					
31145052	0	41	theme	University	26:35	arg1	Solution					50:57	New Baskent University Preservation Solution	14:57	New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia	14:95	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	7	42	theme	P	1183:1183	arg1	<					1185:1185	P < .01	1183:1189	P < .01	1183:1189	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	7	42	theme	P	1183:1183	arg1	UW					1160:1161	UW	1160:1161	UW	1160:1161	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	0	43	theme	Solution	50:57	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.	0:135	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	8	44	theme	HTK	1297:1299	arg1	groups					1308:1313	the HTK and UW groups	1293:1313	groups	1308:1313	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	9	45	theme	HTK	1459:1461	arg1	groups					1472:1477	HTK and BUPS groups	1459:1477	HTK and BUPS groups regarding tubular damage	1459:1502	In experiment 2, after ischemia-reperfusion injury, no significant differences were found between HTK and BUPS groups regarding tubular damage.					
31145052	6	46	theme	perfusion	1035:1043	arg1	hours					1026:1030	up to 3 hours	1018:1030	up to 3 hours of perfusion with any solution	1018:1061	RESULTS In experiment 1, no significant cellular injury was shown at up to 3 hours of perfusion with any solution.					
31145052	3	47	theme	BUPS	447:450	arg1	composition					452:462	MATERIALS AND METHODS BUPS composition	425:462	MATERIALS AND METHODS BUPS composition	425:462	MATERIALS AND METHODS BUPS composition included electrolytes, raffinose, mannitol, N-acetylcysteine, taurine, adenosine, and ascorbic acid.					
31145052	8	48	theme	UW	1305:1306	arg1	groups					1308:1313	the HTK and UW groups	1293:1313	groups	1308:1313	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	11	49	theme	Tubular	1742:1748	arg1	damage					1750:1755	CONCLUSIONS Tubular damage	1730:1755	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis)	1730:1837	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS.					
31145052	12	50	theme	perfusion	1890:1898	arg1	solution					1900:1907	a cost-effective perfusion solution	1873:1907	a cost-effective perfusion solution in transplantation	1873:1926	BUPS can be a cost-effective perfusion solution in transplantation.					
31145052	12	50	theme	perfusion	1890:1898	arg1	BUPS					1861:1864	BUPS	1861:1864	BUPS	1861:1864	BUPS can be a cost-effective perfusion solution in transplantation.					
31145052	9	51	theme	BUPS	1467:1470	arg1	groups					1472:1477	HTK and BUPS groups	1459:1477	HTK and BUPS groups regarding tubular damage	1459:1502	In experiment 2, after ischemia-reperfusion injury, no significant differences were found between HTK and BUPS groups regarding tubular damage.					
31145052	10	52	theme	cytoskeletal	1570:1581	arg1	rearrangment					1583:1594	tubular cytoskeletal rearrangment	1562:1594	tubular cytoskeletal rearrangment	1562:1594	Although no significant differences were shown regarding tubular cytoskeletal rearrangment and apoptosis in pig reperfusion group with BUPS versus HTK, significant differences were shown with these solutions in other groups.					
31145052	12	53	from	solution	1900:1907	arg1	transplantation					1912:1926	transplantation	1912:1926	transplantation	1912:1926	BUPS can be a cost-effective perfusion solution in transplantation.					
31145052	2	54	theme	new	279:281	arg1	BUPS					325:328	the new Baskent University Preservation Solution (BUPS)	275:329	the new Baskent University Preservation Solution (BUPS)	275:329	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	1	55	theme	long	168:171	arg1	time					187:190	long cold ischemia time	168:190	long cold ischemia time	168:190	OBJECTIVES Organ damage due to long cold ischemia time remains a hurdle in transplantation.					
31145052	2	56	theme	Solution	315:322	arg1	BUPS					325:328	the new Baskent University Preservation Solution (BUPS)	275:329	the new Baskent University Preservation Solution (BUPS)	275:329	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	1	57	theme	ischemia	178:185	arg1	time					187:190	long cold ischemia time	168:190	long cold ischemia time	168:190	OBJECTIVES Organ damage due to long cold ischemia time remains a hurdle in transplantation.					
31145052	2	58	theme	University	291:300	arg1	BUPS					325:328	the new Baskent University Preservation Solution (BUPS)	275:329	the new Baskent University Preservation Solution (BUPS)	275:329	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	2	59	theme	Wisconsin	354:362	arg1	UW					365:366	Wisconsin (UW)	354:367	Wisconsin (UW)	354:367	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	4	60	theme	Sprague-Dawley	603:616	arg1	rats					618:621	50 male Sprague-Dawley rats	595:621	50 male Sprague-Dawley rats	595:621	In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation.					
31145052	8	61	theme	48-hour	1332:1338	arg1	perfusion					1340:1348	48-hour perfusion	1332:1348	48-hour perfusion (P < .01)	1332:1358	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	8	61	theme	48-hour	1332:1338	arg1	<					1353:1353	P < .01	1351:1357	P < .01	1351:1357	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	4	62	theme	cold	676:679	arg1	ischemia					681:688	cold ischemia	676:688	cold ischemia injury	676:695	In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation.					
31145052	2	63	theme	animal	249:254	arg1	study					256:260	this preliminary animal study	232:260	this preliminary animal study	232:260	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	1	64	theme	Organ	148:152	arg1	damage					154:159	OBJECTIVES Organ damage	137:159	OBJECTIVES Organ damage due to long cold ischemia time	137:190	OBJECTIVES Organ damage due to long cold ischemia time remains a hurdle in transplantation.					
31145052	0	65	theme	Cold	83:86	arg1	Ischemia					88:95	Cold Ischemia	83:95	Cold Ischemia	83:95	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	5	66	theme	transplant	849:858	arg1	donors					860:865	renal transplant donors	843:865	renal transplant donors to 10 rats	843:876	In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other.					
31145052	6	67	dep	RESULTS	949:955	arg1	shown					1009:1013	shown	1009:1013	was shown at up to 3 hours of perfusion with any solution	1005:1061	RESULTS In experiment 1, no significant cellular injury was shown at up to 3 hours of perfusion with any solution.					
31145052	4	68	theme	UW	654:655	arg1	solution					657:664	UW solution	654:664	UW solution	654:664	In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation.					
31145052	5	69	from	donors	860:865	arg1	experiment					773:782	experiment 2	773:784	experiment 2	773:784	In experiment 2, to investigate ischemia-reperfusion injury, 5 rats were renal transplant donors to 10 rats and 6 pigs were used as transplant donors-recipients among each other.					
31145052	3	70	theme	ascorbic	550:557	arg1	electrolytes					473:484	electrolytes	473:484	electrolytes	473:484	MATERIALS AND METHODS BUPS composition included electrolytes, raffinose, mannitol, N-acetylcysteine, taurine, adenosine, and ascorbic acid.					
31145052	3	70	theme	ascorbic	550:557	arg1	acid					559:562	ascorbic acid	550:562	ascorbic acid	550:562	MATERIALS AND METHODS BUPS composition included electrolytes, raffinose, mannitol, N-acetylcysteine, taurine, adenosine, and ascorbic acid.					
31145052	0	71	theme	Experimental	110:121	arg1	Study					130:134	Preliminary Experimental Animal Study	98:134	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.	0:135	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	4	72	theme	different	721:729	arg1	points					736:741	different time points	721:741	different time points for pathologic evaluation	721:767	In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation.					
31145052	9	73	theme	ischemia-reperfusion	1384:1403	arg1	injury					1405:1410	ischemia-reperfusion injury	1384:1410	ischemia-reperfusion injury	1384:1410	In experiment 2, after ischemia-reperfusion injury, no significant differences were found between HTK and BUPS groups regarding tubular damage.					
31145052	11	74	theme	ischemia-reperfusion	1764:1783	arg1	injury					1785:1790	ischemia-reperfusion injury	1764:1790	ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis)	1764:1837	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS.					
31145052	8	75	theme	moderate	1220:1227	arg1	degree					1229:1234	more moderate degree	1215:1234	more moderate degree of tubular apoptosis	1215:1255	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	8	76	theme	tubular	1239:1245	arg1	apoptosis					1247:1255	tubular apoptosis	1239:1255	tubular apoptosis	1239:1255	The BUPS group showed more moderate degree of tubular apoptosis and cytoskeletal rearrangement than the HTK and UW groups at 12-, 24-, and 48-hour perfusion (P < .01).					
31145052	2	77	theme	HTK	409:411	arg1	solutions					414:422	Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions	354:422	Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions	354:422	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	0	78	theme	Preservation	37:48	arg1	Solution					50:57	New Baskent University Preservation Solution	14:57	New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia	14:95	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	9	79	theme	significant	1416:1426	arg1	differences					1428:1438	no significant differences	1413:1438	no significant differences	1413:1438	In experiment 2, after ischemia-reperfusion injury, no significant differences were found between HTK and BUPS groups regarding tubular damage.					
31145052	6	80	theme	significant	977:987	arg1	injury					998:1003	no significant cellular injury	974:1003	no significant cellular injury	974:1003	RESULTS In experiment 1, no significant cellular injury was shown at up to 3 hours of perfusion with any solution.					
31145052	3	81	theme	MATERIALS	425:433	arg1	composition					452:462	MATERIALS AND METHODS BUPS composition	425:462	MATERIALS AND METHODS BUPS composition	425:462	MATERIALS AND METHODS BUPS composition included electrolytes, raffinose, mannitol, N-acetylcysteine, taurine, adenosine, and ascorbic acid.					
31145052	2	82	theme	histidine-tryptophan-ketoglutarate	373:406	arg1	HTK					409:411	histidine-tryptophan-ketoglutarate (HTK)	373:412	histidine-tryptophan-ketoglutarate (HTK)	373:412	In this preliminary animal study, we compared the new Baskent University Preservation Solution (BUPS) with the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) solutions.					
31145052	11	83	dep	injury	1785:1790	arg1	disruption					1806:1815	cytoskeletal disruption	1793:1815	cytoskeletal disruption	1793:1815	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS.					
31145052	11	83	dep	injury	1785:1790	arg1	apoptosis					1828:1836	increased apoptosis	1818:1836	increased apoptosis	1818:1836	CONCLUSIONS Tubular damage during ischemia-reperfusion injury (cytoskeletal disruption, increased apoptosis) were lower with BUPS.					
31145052	7	84	theme	control	1167:1173	arg1	groups					1175:1180	control groups	1167:1180	control groups	1167:1180	At 6- to 48-hour perfusion, tubular injury was shown, with lowest injury in BUPS and HTK versus UW and control groups (P < .01).					
31145052	0	85	dep	Evaluation	0:9	arg1	Study					130:134	Preliminary Experimental Animal Study	98:134	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.	0:135	Evaluation of New Baskent University Preservation Solution for Kidney Graft During Cold Ischemia: Preliminary Experimental Animal Study.					
31145052	4	86	theme	male	598:601	arg1	rats					618:621	50 male Sprague-Dawley rats	595:621	50 male Sprague-Dawley rats	595:621	In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation.					
31145052	4	87	theme	pathologic	747:756	arg1	evaluation					758:767	pathologic evaluation	747:767	pathologic evaluation	747:767	In experiment 1, kidneys from 50 male Sprague-Dawley rats were placed into BUPS, HTK, or UW solution to assess cold ischemia injury, with biopsies taken at different time points for pathologic evaluation.					
30499275	3	0	theme	initial	720:726	arg1	hours					689:693	the 12th, 72nd and 168th hours	664:693	the 12th, 72nd and 168th hours after inoculation	664:711	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	3	0	theme	initial	720:726	arg1	stage					728:732	the initial stage	716:732	the initial stage	716:732	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	7	1	theme	Clostridium	1085:1095	arg1	abundance					1072:1080	The relative abundance	1059:1080	The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella	1059:1131	The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella increased gradually.					
30499275	11	2	theme	initial	1609:1615	arg1	stage					1617:1621	the initial stage	1605:1621	the initial stage	1605:1621	During cellulose degradation by the microbial consortium, aerobic bacteria were dominant functional bacteria in the initial stage.					
30499275	1	3	theme	community	148:156	arg1	composition					158:168	microbial community composition	138:168	microbial community composition	138:168	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	3	4	theme	end	750:752	arg1	stage					754:758	end stage	750:758	end stage	750:758	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	0	5	theme	microbial	77:85	arg1	consortium					87:96	the microbial consortium	73:96	the microbial consortium	73:96	[Change of bacterial community structure during cellulose degradation by the microbial consortium].					
30499275	3	6	theme	72nd	674:677	arg1	hours					689:693	the 12th, 72nd and 168th hours	664:693	the 12th, 72nd and 168th hours after inoculation	664:711	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	3	6	theme	72nd	674:677	arg1	stage					728:732	the initial stage	716:732	the initial stage	716:732	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	2	7	theme	genes	597:601	arg1	amplicons					564:572	amplicons	564:572	amplicons of bacterial 16 S rRNA genes	564:601	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	11	8	theme	microbial	1529:1537	arg1	consortium					1539:1548	the microbial consortium	1525:1548	the microbial consortium	1525:1548	During cellulose degradation by the microbial consortium, aerobic bacteria were dominant functional bacteria in the initial stage.					
30499275	1	9	theme	composition	158:168	arg1	change					128:133	dynamic change	120:133	dynamic change of microbial community composition	120:168	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	7	10	theme	Cohnella	1124:1131	arg1	abundance					1072:1080	The relative abundance	1059:1080	The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella	1059:1131	The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella increased gradually.					
30499275	1	11	theme	microbial	340:348	arg1	consortium					350:359	the microbial consortium	336:359	the microbial consortium	336:359	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	2	12	theme	rRNA	592:595	arg1	genes					597:601	bacterial 16 S rRNA genes	577:601	bacterial 16 S rRNA genes	577:601	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	1	13	theme	fermentation	427:438	arg1	biomass					409:415	microbial biomass	399:415	microbial biomass	399:415	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	1	13	theme	fermentation	427:438	arg1	pH					421:422	pH	421:422	pH of fermentation broth	421:444	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	1	13	theme	fermentation	427:438	arg1	consortium					350:359	the microbial consortium	336:359	the microbial consortium	336:359	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	1	13	theme	fermentation	427:438	arg1	change					366:371	the change	362:371	the change of substrate degradation	362:396	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	5	14	theme	cellulose	900:908	arg1	degradation					910:920	cellulose degradation	900:920	cellulose degradation	900:920	With cellulose degradation, bacteria in the consortium showed different growth trends.					
30499275	1	15	theme	several	262:268	arg1	aspects					270:276	several aspects	262:276	several aspects of the biodegradation of filter papers and rice straws	262:331	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	2	16	theme	degradation	511:521	arg1	stages					523:528	different degradation stages	501:528	different degradation stages	501:528	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	9	17	theme	Paenibacillus	1277:1289	arg1	abundance					1264:1272	The relative abundance	1251:1272	The relative abundance of Paenibacillus and Ruminococcus	1251:1306	The relative abundance of Paenibacillus and Ruminococcus did not change obviously in each stage.					
30499275	2	18	theme	S	590:590	arg1	genes					597:601	bacterial 16 S rRNA genes	577:601	bacterial 16 S rRNA genes	577:601	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	10	19	theme	main	1367:1370	arg1	all					1379:1381	Above-mentioned 11 main genera all	1348:1381	Above-mentioned 11 main genera all	1348:1381	Above-mentioned 11 main genera all belonged to Firmicutes, which are thermophilic, broad pH adaptable and cellulose or hemicellulose degradable.					
30499275	12	20	theme	anaerobic	1659:1667	arg1	bacteria					1669:1676	anaerobic bacteria	1659:1676	anaerobic bacteria	1659:1676	However, the relative abundance of anaerobic bacteria increased gradually in middle and end stage, and replaced aerobic bacteria to become main bacteria to degrade cellulose.					
30499275	2	21	theme	different	501:509	arg1	stages					523:528	different degradation stages	501:528	different degradation stages	501:528	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	5	22	from	bacteria	923:930	arg1	consortium					939:948	the consortium	935:948	the consortium	935:948	With cellulose degradation, bacteria in the consortium showed different growth trends.					
30499275	7	23	dep	Clostridium	1085:1095	arg1	Bacillus					1098:1105	Clostridium, Bacillus, Geobacillus and Cohnella	1085:1131	Bacillus	1098:1105	The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella increased gradually.					
30499275	12	24	theme	aerobic	1736:1742	arg1	bacteria					1744:1751	aerobic bacteria	1736:1751	aerobic bacteria	1736:1751	However, the relative abundance of anaerobic bacteria increased gradually in middle and end stage, and replaced aerobic bacteria to become main bacteria to degrade cellulose.					
30499275	2	25	from	DNA	466:468	arg1	stages					523:528	different degradation stages	501:528	different degradation stages	501:528	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	7	26	theme	relative	1063:1070	arg1	abundance					1072:1080	The relative abundance	1059:1080	The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella	1059:1131	The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella increased gradually.					
30499275	10	27	dep	Above-mentioned	1348:1362	arg1	11					1364:1365	11	1364:1365	11	1364:1365	Above-mentioned 11 main genera all belonged to Firmicutes, which are thermophilic, broad pH adaptable and cellulose or hemicellulose degradable.					
30499275	6	28	theme	Caloramator	1026:1036	arg1	abundance					995:1003	The relative abundance	982:1003	The relative abundance of Brevibacillus and Caloramator	982:1036	The relative abundance of Brevibacillus and Caloramator decreased gradually.					
30499275	8	29	theme	relative	1158:1165	arg1	abundance					1167:1175	The relative abundance	1154:1175	The relative abundance of Ureibacillus, Tissierella, Epulopiscium	1154:1218	The relative abundance of Ureibacillus, Tissierella, Epulopiscium was the highest in peak stage.					
30499275	8	29	theme	relative	1158:1165	arg1	highest					1228:1234	highest	1228:1234	highest	1228:1234	The relative abundance of Ureibacillus, Tissierella, Epulopiscium was the highest in peak stage.					
30499275	11	30	theme	functional	1582:1591	arg1	bacteria					1593:1600	dominant functional bacteria	1573:1600	dominant functional bacteria	1573:1600	During cellulose degradation by the microbial consortium, aerobic bacteria were dominant functional bacteria in the initial stage.					
30499275	3	31	theme	12th	668:671	arg1	hours					689:693	the 12th, 72nd and 168th hours	664:693	the 12th, 72nd and 168th hours after inoculation	664:711	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	3	31	theme	12th	668:671	arg1	stage					728:732	the initial stage	716:732	the initial stage	716:732	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	1	32	theme	key	186:188	arg1	bacteria					201:208	key functional bacteria	186:208	key functional bacteria in the cellulose degradation consortium	186:248	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	0	33	theme	community	21:29	arg1	structure					31:39	bacterial community structure	11:39	bacterial community structure	11:39	[Change of bacterial community structure during cellulose degradation by the microbial consortium].					
30499275	3	34	theme	peak	735:738	arg1	stage					740:744	peak stage	735:744	peak stage	735:744	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	11	35	theme	aerobic	1551:1557	arg1	bacteria					1559:1566	aerobic bacteria	1551:1566	aerobic bacteria	1551:1566	During cellulose degradation by the microbial consortium, aerobic bacteria were dominant functional bacteria in the initial stage.					
30499275	1	36	theme	functional	190:199	arg1	bacteria					201:208	key functional bacteria	186:208	key functional bacteria in the cellulose degradation consortium	186:248	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	0	37	theme	bacterial	11:19	arg1	structure					31:39	bacterial community structure	11:39	bacterial community structure	11:39	[Change of bacterial community structure during cellulose degradation by the microbial consortium].					
30499275	1	38	theme	biodegradation	285:298	arg1	aspects					270:276	several aspects	262:276	several aspects of the biodegradation of filter papers and rice straws	262:331	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	11	39	theme	dominant	1573:1580	arg1	bacteria					1593:1600	dominant functional bacteria	1573:1600	dominant functional bacteria	1573:1600	During cellulose degradation by the microbial consortium, aerobic bacteria were dominant functional bacteria in the initial stage.					
30499275	12	40	theme	relative	1637:1644	arg1	abundance					1646:1654	the relative abundance	1633:1654	the relative abundance of anaerobic bacteria	1633:1676	However, the relative abundance of anaerobic bacteria increased gradually in middle and end stage, and replaced aerobic bacteria to become main bacteria to degrade cellulose.					
30499275	7	41	theme	Geobacillus	1108:1118	arg1	abundance					1072:1080	The relative abundance	1059:1080	The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella	1059:1131	The relative abundance of Clostridium, Bacillus, Geobacillus and Cohnella increased gradually.					
30499275	2	42	theme	high-throughput	534:548	arg1	sequencing					550:559	high-throughput sequencing	534:559	high-throughput sequencing of amplicons of bacterial 16 S rRNA genes	534:601	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	1	43	theme	substrate	376:384	arg1	degradation					386:396	substrate degradation	376:396	substrate degradation	376:396	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	5	44	theme	different	957:965	arg1	trends					974:979	different growth trends	957:979	different growth trends	957:979	With cellulose degradation, bacteria in the consortium showed different growth trends.					
30499275	6	45	theme	Brevibacillus	1008:1020	arg1	abundance					995:1003	The relative abundance	982:1003	The relative abundance of Brevibacillus and Caloramator	982:1036	The relative abundance of Brevibacillus and Caloramator decreased gradually.					
30499275	6	46	theme	relative	986:993	arg1	abundance					995:1003	The relative abundance	982:1003	The relative abundance of Brevibacillus and Caloramator	982:1036	The relative abundance of Brevibacillus and Caloramator decreased gradually.					
30499275	0	47	theme	structure	31:39	arg1	[Change					0:6	[Change	0:6	[Change of bacterial community structure during cellulose degradation by the microbial consortium	0:96	[Change of bacterial community structure during cellulose degradation by the microbial consortium].					
30499275	1	48	theme	filter	303:308	arg1	papers					310:315	filter papers	303:315	filter papers	303:315	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	2	49	theme	total	460:464	arg1	DNA					466:468	total DNA	460:468	total DNA of the microbial consortium in different degradation stages	460:528	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	1	50	theme	degradation	386:396	arg1	biomass					409:415	microbial biomass	399:415	microbial biomass	399:415	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	1	50	theme	degradation	386:396	arg1	pH					421:422	pH	421:422	pH of fermentation broth	421:444	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	1	50	theme	degradation	386:396	arg1	consortium					350:359	the microbial consortium	336:359	the microbial consortium	336:359	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	1	50	theme	degradation	386:396	arg1	change					366:371	the change	362:371	the change of substrate degradation	362:396	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	5	51	theme	growth	967:972	arg1	trends					974:979	different growth trends	957:979	different growth trends	957:979	With cellulose degradation, bacteria in the consortium showed different growth trends.					
30499275	12	52	theme	main	1763:1766	arg1	bacteria					1768:1775	main bacteria	1763:1775	main bacteria	1763:1775	However, the relative abundance of anaerobic bacteria increased gradually in middle and end stage, and replaced aerobic bacteria to become main bacteria to degrade cellulose.					
30499275	1	53	theme	papers	310:315	arg1	biodegradation					285:298	the biodegradation	281:298	the biodegradation of filter papers and rice straws	281:331	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	2	54	theme	16	587:588	arg1	S					590:590	S	590:590	S	590:590	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	12	55	theme	bacteria	1669:1676	arg1	abundance					1646:1654	the relative abundance	1633:1654	the relative abundance of anaerobic bacteria	1633:1676	However, the relative abundance of anaerobic bacteria increased gradually in middle and end stage, and replaced aerobic bacteria to become main bacteria to degrade cellulose.					
30499275	11	56	from	bacteria	1593:1600	arg1	stage					1617:1621	the initial stage	1605:1621	the initial stage	1605:1621	During cellulose degradation by the microbial consortium, aerobic bacteria were dominant functional bacteria in the initial stage.					
30499275	9	57	theme	Ruminococcus	1295:1306	arg1	abundance					1264:1272	The relative abundance	1251:1272	The relative abundance of Paenibacillus and Ruminococcus	1251:1306	The relative abundance of Paenibacillus and Ruminococcus did not change obviously in each stage.					
30499275	10	58	dep	degradable	1481:1490	arg1	cellulose					1454:1462	cellulose	1454:1462	cellulose	1454:1462	Above-mentioned 11 main genera all belonged to Firmicutes, which are thermophilic, broad pH adaptable and cellulose or hemicellulose degradable.					
30499275	0	59	theme	cellulose	48:56	arg1	degradation					58:68	cellulose degradation	48:68	cellulose degradation by the microbial consortium	48:96	[Change of bacterial community structure during cellulose degradation by the microbial consortium].					
30499275	10	60	theme	genera	1372:1377	arg1	all					1379:1381	Above-mentioned 11 main genera all	1348:1381	Above-mentioned 11 main genera all	1348:1381	Above-mentioned 11 main genera all belonged to Firmicutes, which are thermophilic, broad pH adaptable and cellulose or hemicellulose degradable.					
30499275	2	61	theme	bacterial	577:585	arg1	genes					597:601	bacterial 16 S rRNA genes	577:601	bacterial 16 S rRNA genes	577:601	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	1	62	theme	microbial	399:407	arg1	biomass					409:415	microbial biomass	399:415	microbial biomass	399:415	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	4	63	theme	microbial	794:802	arg1	consortium					804:813	The microbial consortium	790:813	The microbial consortium	790:813	The microbial consortium was mainly composed of 1 phylum, 2 classes, 2 orders, 7 families and 11 genera.					
30499275	9	64	theme	relative	1255:1262	arg1	abundance					1264:1272	The relative abundance	1251:1272	The relative abundance of Paenibacillus and Ruminococcus	1251:1306	The relative abundance of Paenibacillus and Ruminococcus did not change obviously in each stage.					
30499275	3	65	theme	168th	683:687	arg1	hours					689:693	the 12th, 72nd and 168th hours	664:693	the 12th, 72nd and 168th hours after inoculation	664:711	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	3	65	theme	168th	683:687	arg1	stage					728:732	the initial stage	716:732	the initial stage	716:732	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	12	66	theme	end	1712:1714	arg1	stage					1716:1720	end stage	1712:1720	end stage	1712:1720	However, the relative abundance of anaerobic bacteria increased gradually in middle and end stage, and replaced aerobic bacteria to become main bacteria to degrade cellulose.					
30499275	1	67	theme	rice	321:324	arg1	straws					326:331	rice straws	321:331	rice straws	321:331	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	1	68	from	bacteria	201:208	arg1	consortium					239:248	the cellulose degradation consortium	213:248	the cellulose degradation consortium	213:248	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	1	69	theme	dynamic	120:126	arg1	change					128:133	dynamic change	120:133	dynamic change of microbial community composition	120:168	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	3	70	dep	characteristics	631:645	arg1	test					647:650	test	647:650	test	647:650	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	1	71	theme	cellulose	217:225	arg1	consortium					239:248	the cellulose degradation consortium	213:248	the cellulose degradation consortium	213:248	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	2	72	theme	amplicons	564:572	arg1	sequencing					550:559	high-throughput sequencing	534:559	high-throughput sequencing of amplicons of bacterial 16 S rRNA genes	534:601	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	3	73	theme	decomposition	617:629	arg1	characteristics					631:645	the decomposition characteristics	613:645	the decomposition characteristics test	613:650	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	10	74	theme	Above-mentioned	1348:1362	arg1	all					1379:1381	Above-mentioned 11 main genera all	1348:1381	Above-mentioned 11 main genera all	1348:1381	Above-mentioned 11 main genera all belonged to Firmicutes, which are thermophilic, broad pH adaptable and cellulose or hemicellulose degradable.					
30499275	1	75	theme	degradation	227:237	arg1	consortium					239:248	the cellulose degradation consortium	213:248	the cellulose degradation consortium	213:248	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	2	76	theme	consortium	487:496	arg1	DNA					466:468	total DNA	460:468	total DNA of the microbial consortium in different degradation stages	460:528	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	8	77	theme	peak	1239:1242	arg1	stage					1244:1248	peak stage	1239:1248	peak stage	1239:1248	The relative abundance of Ureibacillus, Tissierella, Epulopiscium was the highest in peak stage.					
30499275	8	78	theme	Epulopiscium	1207:1218	arg1	abundance					1167:1175	The relative abundance	1154:1175	The relative abundance of Ureibacillus, Tissierella, Epulopiscium	1154:1218	The relative abundance of Ureibacillus, Tissierella, Epulopiscium was the highest in peak stage.					
30499275	8	78	theme	Epulopiscium	1207:1218	arg1	highest					1228:1234	highest	1228:1234	highest	1228:1234	The relative abundance of Ureibacillus, Tissierella, Epulopiscium was the highest in peak stage.					
30499275	2	79	theme	microbial	477:485	arg1	consortium					487:496	the microbial consortium	473:496	the microbial consortium	473:496	We extracted total DNA of the microbial consortium in different degradation stages for high-throughput sequencing of amplicons of bacterial 16 S rRNA genes.					
30499275	1	80	theme	straws	326:331	arg1	biodegradation					285:298	the biodegradation	281:298	the biodegradation of filter papers and rice straws	281:331	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	1	81	theme	microbial	138:146	arg1	composition					158:168	microbial community composition	138:168	microbial community composition	138:168	In order to clarify dynamic change of microbial community composition and to identify key functional bacteria in the cellulose degradation consortium, we studied several aspects of the biodegradation of filter papers and rice straws by the microbial consortium, the change of substrate degradation, microbial biomass and pH of fermentation broth.					
30499275	11	82	theme	cellulose	1500:1508	arg1	degradation					1510:1520	cellulose degradation	1500:1520	cellulose degradation by the microbial consortium	1500:1548	During cellulose degradation by the microbial consortium, aerobic bacteria were dominant functional bacteria in the initial stage.					
30499275	3	83	theme	degradation	763:773	arg1	hours					689:693	the 12th, 72nd and 168th hours	664:693	the 12th, 72nd and 168th hours after inoculation	664:711	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	3	83	theme	degradation	763:773	arg1	stage					728:732	the initial stage	716:732	the initial stage	716:732	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	3	83	theme	degradation	763:773	arg1	stage					740:744	peak stage	735:744	peak stage	735:744	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
30499275	3	83	theme	degradation	763:773	arg1	stage					754:758	end stage	750:758	end stage	750:758	Based on the decomposition characteristics test, we defined the 12th, 72nd and 168th hours after inoculation as the initial stage, peak stage and end stage of degradation, respectively.					
29553005	6	0	theme	rumen	883:887	arg1	pH					889:890	lower (P<0.05) rumen pH	868:890	lower (P<0.05) rumen pH	868:890	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	6	1	dep	rumen	1023:1027	arg1	the					1019:1021	the	1019:1021	the	1019:1021	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	4	2	theme	grain	643:647	arg1	diet					634:637	a hay diet	628:637	a hay diet (0% grain; n=6)	628:653	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	4	2	theme	grain	643:647	arg1	n=6					650:652	0% grain; n=6	640:652	0% grain; n=6	640:652	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	8	3	dep	increased	1225:1233	arg1	P<0.05					1236:1241	P<0.05	1236:1241	P<0.05	1236:1241	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	6	4	theme	lactate	943:949	arg1	levels					1009:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	5	5	theme	treatment	702:710	arg1	day					692:694	day 50	692:697	day 50 of treatment	692:710	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	6	6	theme	acids	933:937	arg1	levels					1009:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	4	7	theme	male	608:611	arg1	goats					613:617	12 male goats	605:617	12 male goats	605:617	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	3	8	theme	cytokines	526:534	arg1	expression					495:504	the expression	491:504	the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue	491:594	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	1	9	theme	intensive	165:173	arg1	system					186:191	the current intensive production system	153:191	the current intensive production system	153:191	In the current intensive production system, ruminants are often fed high-grain (HG) diets.					
29553005	6	10	theme	volatile	918:925	arg1	acids					933:937	volatile fatty acids	918:937	volatile fatty acids	918:937	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	10	11	theme	higher	1704:1709	arg1	levels					1711:1716	higher levels	1704:1716	higher levels of LPS in the peripheral blood	1704:1747	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	2	12	theme	feeding	255:261	arg1	pattern					263:269	this feeding pattern	250:269	this feeding pattern	250:269	However, this feeding pattern often causes rumen metabolic disorders and may further trigger laminitis, the exact mechanism is not clear.					
29553005	9	13	theme	pro-inflammatory	1440:1455	arg1	cytokines					1457:1465	pro-inflammatory cytokines	1440:1465	pro-inflammatory cytokines	1440:1465	Correlation analysis indicated that the expression of pro-inflammatory cytokines were positively correlated with MMP-2 expression in lamellar tissues.					
29553005	10	14	theme	damage	1862:1867	arg1	level					1845:1849	the level	1841:1849	the level of laminar damage	1841:1867	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	10	15	theme	peripheral	1732:1741	arg1	blood					1743:1747	the peripheral blood	1728:1747	the peripheral blood	1728:1747	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	3	16	theme	matrix	540:545	arg1	metalloproteinases					547:564	matrix metalloproteinases	540:564	matrix metalloproteinases (MMPs)	540:571	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	3	16	theme	matrix	540:545	arg1	MMPs					567:570	MMPs	567:570	MMPs	567:570	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	7	17	theme	community	1099:1107	arg1	composition					1068:1078	the composition	1064:1078	the composition of rumen bacterial community	1064:1107	HG diet feeding altered the composition of rumen bacterial community, and correspondingly, the results suggested that their functions in the HG group were also altered.					
29553005	0	18	theme	goats	143:147	arg1	tissues					132:138	the laminar tissues	120:138	the laminar tissues of goats	120:147	High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats.					
29553005	5	19	theme	lamellar	750:757	arg1	tissue					759:764	lamellar tissue	750:764	lamellar tissue	750:764	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	9	20	theme	MMP-2	1499:1503	arg1	expression					1505:1514	MMP-2 expression	1499:1514	MMP-2 expression in lamellar tissues	1499:1534	Correlation analysis indicated that the expression of pro-inflammatory cytokines were positively correlated with MMP-2 expression in lamellar tissues.					
29553005	8	21	theme	diet	1212:1215	arg1	feeding					1217:1223	HG diet feeding	1209:1223	HG diet feeding	1209:1223	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	7	22	theme	rumen	1083:1087	arg1	community					1099:1107	rumen bacterial community	1083:1107	rumen bacterial community	1083:1107	HG diet feeding altered the composition of rumen bacterial community, and correspondingly, the results suggested that their functions in the HG group were also altered.					
29553005	10	23	theme	HG	1574:1575	arg1	feeding					1577:1583	HG feeding	1574:1583	HG feeding	1574:1583	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	5	24	theme	blood	724:728	arg1	samples					713:719	samples	713:719	samples of blood, rumen content, and lamellar tissue of hooves of goats	713:783	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	7	25	from	functions	1164:1172	arg1	group					1184:1188	the HG group	1177:1188	the HG group	1177:1188	HG diet feeding altered the composition of rumen bacterial community, and correspondingly, the results suggested that their functions in the HG group were also altered.					
29553005	0	26	theme	rumen	69:73	arg1	community					85:93	the rumen bacterial community	65:93	the rumen bacterial community	65:93	High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats.					
29553005	5	27	theme	rumen	731:735	arg1	content					737:743	rumen content	731:743	rumen content	731:743	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	0	28	theme	community	85:93	arg1	composition					36:46	composition	36:46	composition	36:46	High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats.					
29553005	0	28	theme	community	85:93	arg1	functions					52:60	functions	52:60	functions	52:60	High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats.					
29553005	10	29	from	levels	1711:1716	arg1	blood					1743:1747	the peripheral blood	1728:1747	the peripheral blood	1728:1747	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	4	30	theme	HG	661:662	arg1	diet					664:667	an HG diet	658:667	an HG diet (56.5% grain; n=6)	658:686	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	4	30	theme	HG	661:662	arg1	n=6					683:685	56.5% grain; n=6	670:685	56.5% grain; n=6	670:685	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	6	31	dep	lower	868:872	arg1	P<0.05					875:880	P<0.05	875:880	P<0.05	875:880	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	0	32	theme	High-grain	0:9	arg1	feeding					16:22	High-grain diet feeding	0:22	High-grain diet feeding	0:22	High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats.					
29553005	3	33	theme	HG	417:418	arg1	feeding					425:431	HG diet feeding	417:431	HG diet feeding	417:431	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	6	34	theme	HG-fed	851:856	arg1	goats					858:862	HG-fed goats	851:862	HG-fed goats	851:862	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	10	35	theme	rumen	1609:1613	arg1	fermentation					1615:1626	rumen fermentation	1609:1626	rumen fermentation	1609:1626	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	7	36	theme	HG	1040:1041	arg1	feeding					1048:1054	HG diet feeding	1040:1054	HG diet feeding	1040:1054	HG diet feeding altered the composition of rumen bacterial community, and correspondingly, the results suggested that their functions in the HG group were also altered.					
29553005	8	37	theme	necrosis	1300:1307	arg1	factor-α					1309:1316	tumour necrosis factor-α	1293:1316	tumour necrosis factor-α	1293:1316	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	3	38	theme	fermentative	436:447	arg1	changes					463:469	fermentative and microbial changes	436:469	fermentative and microbial changes in the rumen	436:482	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	6	39	theme	rumen	958:962	arg1	LPS					1004:1006	LPS	1004:1006	LPS	1004:1006	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	6	39	theme	rumen	958:962	arg1	lipopolysaccharide					984:1001	the rumen and higher (P<0.05) lipopolysaccharide	954:1001	the rumen and higher (P<0.05) lipopolysaccharide (LPS)	954:1007	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	2	40	theme	metabolic	290:298	arg1	disorders					300:308	rumen metabolic disorders	284:308	rumen metabolic disorders	284:308	However, this feeding pattern often causes rumen metabolic disorders and may further trigger laminitis, the exact mechanism is not clear.					
29553005	8	41	theme	hay	1375:1377	arg1	group					1379:1383	the hay group	1371:1383	the hay group	1371:1383	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	6	42	theme	higher	896:901	arg1	levels					1009:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	10	43	theme	lamellar	1801:1808	arg1	tissues					1810:1816	lamellar tissues	1801:1816	lamellar tissues	1801:1816	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	8	44	theme	mRNA	1328:1331	arg1	expression					1248:1257	the expression	1244:1257	the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues	1244:1355	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	4	45	theme	%	674:674	arg1	diet					664:667	an HG diet	658:667	an HG diet (56.5% grain; n=6)	658:686	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	4	45	theme	%	674:674	arg1	n=6					683:685	56.5% grain; n=6	670:685	56.5% grain; n=6	670:685	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	10	46	dep	composition	1636:1646	arg1	the					1632:1634	the	1632:1634	the	1632:1634	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	10	47	theme	rumen	1665:1669	arg1	community					1681:1689	rumen bacterial community	1665:1689	rumen bacterial community	1665:1689	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	3	48	theme	microbial	453:461	arg1	changes					463:469	fermentative and microbial changes	436:469	fermentative and microbial changes in the rumen	436:482	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	3	49	from	changes	463:469	arg1	rumen					478:482	the rumen	474:482	the rumen	474:482	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	9	50	theme	Correlation	1386:1396	arg1	analysis					1398:1405	Correlation analysis	1386:1405	Correlation analysis	1386:1405	Correlation analysis indicated that the expression of pro-inflammatory cytokines were positively correlated with MMP-2 expression in lamellar tissues.					
29553005	7	51	theme	HG	1181:1182	arg1	group					1184:1188	the HG group	1177:1188	the HG group	1177:1188	HG diet feeding altered the composition of rumen bacterial community, and correspondingly, the results suggested that their functions in the HG group were also altered.					
29553005	10	52	theme	laminar	1854:1860	arg1	damage					1862:1867	laminar damage	1854:1867	laminar damage	1854:1867	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	10	53	theme	community	1681:1689	arg1	patterns					1597:1604	the patterns	1593:1604	the patterns of rumen fermentation	1593:1626	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	10	53	theme	community	1681:1689	arg1	composition					1636:1646	composition	1636:1646	composition	1636:1646	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	10	53	theme	community	1681:1689	arg1	functions					1652:1660	functions	1652:1660	functions	1652:1660	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	3	54	theme	pro-inflammatory	509:524	arg1	cytokines					526:534	pro-inflammatory cytokines	509:534	pro-inflammatory cytokines	509:534	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	1	55	theme	current	157:163	arg1	system					186:191	the current intensive production system	153:191	the current intensive production system	153:191	In the current intensive production system, ruminants are often fed high-grain (HG) diets.					
29553005	6	56	theme	fatty	927:931	arg1	acids					933:937	volatile fatty acids	918:937	volatile fatty acids	918:937	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	1	57	theme	production	175:184	arg1	system					186:191	the current intensive production system	153:191	the current intensive production system	153:191	In the current intensive production system, ruminants are often fed high-grain (HG) diets.					
29553005	6	58	theme	total	912:916	arg1	levels					1009:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	0	59	theme	laminar	124:130	arg1	tissues					132:138	the laminar tissues	120:138	the laminar tissues of goats	120:147	High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats.					
29553005	3	60	theme	metalloproteinases	547:564	arg1	expression					495:504	the expression	491:504	the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue	491:594	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	9	61	theme	cytokines	1457:1465	arg1	expression					1426:1435	the expression	1422:1435	the expression of pro-inflammatory cytokines	1422:1465	Correlation analysis indicated that the expression of pro-inflammatory cytokines were positively correlated with MMP-2 expression in lamellar tissues.					
29553005	5	62	theme	content	737:743	arg1	samples					713:719	samples	713:719	samples of blood, rumen content, and lamellar tissue of hooves of goats	713:783	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	8	63	theme	HG	1209:1210	arg1	feeding					1217:1223	HG diet feeding	1209:1223	HG diet feeding	1209:1223	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	4	64	theme	hay	630:632	arg1	diet					634:637	a hay diet	628:637	a hay diet (0% grain; n=6)	628:653	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	4	64	theme	hay	630:632	arg1	n=6					650:652	0% grain; n=6	640:652	0% grain; n=6	640:652	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	3	65	from	expression	495:504	arg1	tissue					589:594	the lamellar tissue	576:594	the lamellar tissue	576:594	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	10	66	theme	LPS	1721:1723	arg1	levels					1711:1716	higher levels	1704:1716	higher levels of LPS in the peripheral blood	1704:1747	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	6	67	theme	hay	840:842	arg1	group					844:848	the hay group	836:848	the hay group	836:848	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	6	68	theme	higher	968:973	arg1	LPS					1004:1006	LPS	1004:1006	LPS	1004:1006	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	6	68	theme	higher	968:973	arg1	lipopolysaccharide					984:1001	the rumen and higher (P<0.05) lipopolysaccharide	954:1001	the rumen and higher (P<0.05) lipopolysaccharide (LPS)	954:1007	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	6	69	dep	higher	968:973	arg1	P<0.05					976:981	P<0.05	976:981	P<0.05	976:981	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	3	70	theme	feeding	425:431	arg1	effect					407:412	the effect	403:412	the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue	403:594	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	6	71	contain	had	864:866	arg1	goats					858:862	HG-fed goats	851:862	HG-fed goats	851:862	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	6	71	contain	had	864:866	arg2	levels					1009:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels	896:1014	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	6	71	contain	had	864:866	arg2	pH					889:890	lower (P<0.05) rumen pH	868:890	lower (P<0.05) rumen pH	868:890	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	7	72	theme	bacterial	1089:1097	arg1	community					1099:1107	rumen bacterial community	1083:1107	rumen bacterial community	1083:1107	HG diet feeding altered the composition of rumen bacterial community, and correspondingly, the results suggested that their functions in the HG group were also altered.					
29553005	0	73	theme	bacterial	75:83	arg1	community					85:93	the rumen bacterial community	65:93	the rumen bacterial community	65:93	High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats.					
29553005	9	74	theme	lamellar	1519:1526	arg1	tissues					1528:1534	lamellar tissues	1519:1534	lamellar tissues	1519:1534	Correlation analysis indicated that the expression of pro-inflammatory cytokines were positively correlated with MMP-2 expression in lamellar tissues.					
29553005	10	75	theme	inflammatory	1776:1787	arg1	response					1789:1796	the inflammatory response	1772:1796	the inflammatory response	1772:1796	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	5	76	theme	goats	779:783	arg1	hooves					769:774	hooves	769:774	hooves of goats	769:783	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	6	77	from	lactate	943:949	arg1	LPS					1004:1006	LPS	1004:1006	LPS	1004:1006	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	6	77	from	lactate	943:949	arg1	lipopolysaccharide					984:1001	the rumen and higher (P<0.05) lipopolysaccharide	954:1001	the rumen and higher (P<0.05) lipopolysaccharide (LPS)	954:1007	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	8	78	theme	interleukin-1β	1262:1275	arg1	expression					1248:1257	the expression	1244:1257	the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues	1244:1355	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	2	79	theme	exact	349:353	arg1	mechanism					355:363	the exact mechanism	345:363	the exact mechanism	345:363	However, this feeding pattern often causes rumen metabolic disorders and may further trigger laminitis, the exact mechanism is not clear.					
29553005	2	79	theme	exact	349:353	arg1	clear					372:376	clear	372:376	clear	372:376	However, this feeding pattern often causes rumen metabolic disorders and may further trigger laminitis, the exact mechanism is not clear.					
29553005	0	80	theme	diet	11:14	arg1	feeding					16:22	High-grain diet feeding	0:22	High-grain diet feeding	0:22	High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats.					
29553005	5	81	theme	tissue	759:764	arg1	samples					713:719	samples	713:719	samples of blood, rumen content, and lamellar tissue of hooves of goats	713:783	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	6	82	theme	lower	868:872	arg1	pH					889:890	lower (P<0.05) rumen pH	868:890	lower (P<0.05) rumen pH	868:890	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	8	83	theme	interleukin-6	1278:1290	arg1	expression					1248:1257	the expression	1244:1257	the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues	1244:1355	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	4	84	theme	%	641:641	arg1	diet					634:637	a hay diet	628:637	a hay diet (0% grain; n=6)	628:653	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	4	84	theme	%	641:641	arg1	n=6					650:652	0% grain; n=6	640:652	0% grain; n=6	640:652	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	5	85	theme	hooves	769:774	arg1	tissue					759:764	lamellar tissue	750:764	lamellar tissue	750:764	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	5	85	theme	hooves	769:774	arg1	blood					724:728	blood	724:728	blood	724:728	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	5	85	theme	hooves	769:774	arg1	content					737:743	rumen content	731:743	rumen content	731:743	On day 50 of treatment, samples of blood, rumen content, and lamellar tissue of hooves of goats were collected.					
29553005	7	86	theme	diet	1043:1046	arg1	feeding					1048:1054	HG diet feeding	1040:1054	HG diet feeding	1040:1054	HG diet feeding altered the composition of rumen bacterial community, and correspondingly, the results suggested that their functions in the HG group were also altered.					
29553005	8	87	theme	tumour	1293:1298	arg1	factor-α					1309:1316	tumour necrosis factor-α	1293:1316	tumour necrosis factor-α	1293:1316	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	10	88	theme	fermentation	1615:1626	arg1	patterns					1597:1604	the patterns	1593:1604	the patterns of rumen fermentation	1593:1626	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	10	88	theme	fermentation	1615:1626	arg1	composition					1636:1646	composition	1636:1646	composition	1636:1646	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	10	88	theme	fermentation	1615:1626	arg1	functions					1652:1660	functions	1652:1660	functions	1652:1660	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	6	89	from	acids	933:937	arg1	LPS					1004:1006	LPS	1004:1006	LPS	1004:1006	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	6	89	from	acids	933:937	arg1	lipopolysaccharide					984:1001	the rumen and higher (P<0.05) lipopolysaccharide	954:1001	the rumen and higher (P<0.05) lipopolysaccharide (LPS)	954:1007	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	3	90	theme	diet	420:423	arg1	feeding					425:431	HG diet feeding	417:431	HG diet feeding	417:431	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	8	91	theme	factor-α	1309:1316	arg1	expression					1248:1257	the expression	1244:1257	the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues	1244:1355	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	3	92	theme	lamellar	580:587	arg1	tissue					589:594	the lamellar tissue	576:594	the lamellar tissue	576:594	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	4	93	theme	grain	676:680	arg1	diet					664:667	an HG diet	658:667	an HG diet (56.5% grain; n=6)	658:686	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	4	93	theme	grain	676:680	arg1	n=6					683:685	56.5% grain; n=6	670:685	56.5% grain; n=6	670:685	In all, 12 male goats were fed a hay diet (0% grain; n=6) or an HG diet (56.5% grain; n=6).					
29553005	3	94	from	effect	407:412	arg1	changes					463:469	fermentative and microbial changes	436:469	fermentative and microbial changes in the rumen	436:482	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	3	94	from	effect	407:412	arg1	expression					495:504	the expression	491:504	the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue	491:594	This study investigated the effect of HG diet feeding on fermentative and microbial changes in the rumen and on the expression of pro-inflammatory cytokines and matrix metalloproteinases (MMPs) in the lamellar tissue.					
29553005	8	95	from	expression	1248:1257	arg1	tissues					1349:1355	the lamellar tissues	1336:1355	the lamellar tissues	1336:1355	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	0	96	dep	composition	36:46	arg1	the					32:34	the	32:34	the	32:34	High-grain diet feeding altered the composition and functions of the rumen bacterial community and caused the damage to the laminar tissues of goats.					
29553005	8	97	theme	MMP-2	1322:1326	arg1	mRNA					1328:1331	MMP-2 mRNA	1322:1331	MMP-2 mRNA	1322:1331	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	6	98	dep	higher	896:901	arg1	P<0.05					904:909	P<0.05	904:909	P<0.05	904:909	The data showed that compared with the hay group, HG-fed goats had lower (P<0.05) rumen pH but higher (P<0.05) total volatile fatty acids and lactate in the rumen and higher (P<0.05) lipopolysaccharide (LPS) levels in the rumen and blood.					
29553005	2	99	theme	rumen	284:288	arg1	disorders					300:308	rumen metabolic disorders	284:308	rumen metabolic disorders	284:308	However, this feeding pattern often causes rumen metabolic disorders and may further trigger laminitis, the exact mechanism is not clear.					
29553005	9	100	from	expression	1505:1514	arg1	tissues					1528:1534	lamellar tissues	1519:1534	lamellar tissues	1519:1534	Correlation analysis indicated that the expression of pro-inflammatory cytokines were positively correlated with MMP-2 expression in lamellar tissues.					
29553005	8	101	theme	lamellar	1340:1347	arg1	tissues					1349:1355	the lamellar tissues	1336:1355	the lamellar tissues	1336:1355	HG diet feeding increased (P<0.05) the expression of interleukin-1β, interleukin-6, tumour necrosis factor-α and MMP-2 mRNA in the lamellar tissues compared with the hay group.					
29553005	10	102	theme	bacterial	1671:1679	arg1	community					1681:1689	rumen bacterial community	1665:1689	rumen bacterial community	1665:1689	Overall, these results revealed that HG feeding altered the patterns of rumen fermentation and the composition and functions of rumen bacterial community, and lead to higher levels of LPS in the peripheral blood, and further activated the inflammatory response in lamellar tissues, which may progress to the level of laminar damage.					
29553005	1	103	theme	high-grain	218:227	arg1	diets					234:238	high-grain (HG) diets	218:238	high-grain (HG) diets	218:238	In the current intensive production system, ruminants are often fed high-grain (HG) diets.					
30711662	14	0	theme	minimal	2564:2570	arg1	effects					2572:2578	minimal effects	2564:2578	minimal effects on primary cell lines	2564:2600	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	3	1	theme	carbon	678:683	arg1	shells					685:690	carbon shells	678:690	carbon shells	678:690	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	11	2	theme	systemic	1952:1959	arg1	effects					1961:1967	no adverse systemic effects	1941:1967	no adverse systemic effects	1941:1967	The passage was noninvasive and caused no adverse systemic effects to the animals.					
30711662	1	3	theme	brain	254:258	arg1	tumors					260:265	brain tumors	254:265	brain tumors	254:265	Despite the advances in molecularly targeted therapies, delivery across the blood-brain barrier (BBB) and the targeting of brain tumors remains a challenge.					
30711662	16	4	theme	barrier	2887:2893	arg1	models					2861:2866	Both in vitro and in vivo models	2835:2866	Both in vitro and in vivo models of the blood-brain barrier	2835:2893	Both in vitro and in vivo models of the blood-brain barrier evidence the ability of nanoparticles to cross the barrier and localize to the brain tissue.					
30711662	3	5	contain	containing	579:588	arg1	ferrofluid					568:577	an aqueous, surfactant-free ferrofluid	540:577	an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells	540:690	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	3	5	contain	containing	579:588	arg2	nanoparticles					619:631	superparamagnetic iron oxide nanoparticles	590:631	superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells	590:690	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	3	5	contain	containing	579:588	arg2	SPIONs					634:639	SPIONs	634:639	SPIONs	634:639	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	3	6	theme	earthicle	515:523	arg1	concept					500:506	the previously proposed concept	476:506	the previously proposed concept of the earthicle	476:523	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	15	7	theme	tumor	2708:2712	arg1	viability					2714:2722	the tumor viability	2704:2722	the tumor viability	2704:2722	In 3D tumor spheroids, nanoparticles greatly reduced the metastatic migration of cancer cells, while the tumor viability was reduced compared to the control group by applying magnetic hyperthermia to nanoparticle-treated spheroids.					
30711662	9	8	theme	MDCK-MDR1	1470:1478	arg1	model					1484:1488	MDCK-MDR1 BBB model	1470:1488	MDCK-MDR1 BBB model	1470:1488	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	13	9	theme	ferrofluid-infused	2314:2331	arg1	media					2333:2337	ferrofluid-infused media	2314:2337	ferrofluid-infused media	2314:2337	They are also effective against solid tumors in vitro, can cross the BBB in Drosophila, and are nontoxic based on the developmental studies of flies raised in ferrofluid-infused media.					
30711662	14	10	theme	composite	2423:2431	arg1	ferrofluid					2452:2461	a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid	2387:2461	a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid	2387:2461	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	14	10	theme	composite	2423:2431	arg1	effective					2466:2474	effective	2466:2474	effective	2466:2474	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	17	11	theme	cancer	3106:3111	arg1	types					3097:3101	different types	3087:3101	different types of cancer	3087:3111	These composite nanoparticles show great promise as an anticancer biomaterial for the treatment of different types of cancer and may serve as an alternative or addendum to traditional chemotherapies.					
30711662	8	12	dep	sufficient	1415:1424	arg1	cause					1434:1438	cause	1434:1438	to cause tumor death	1431:1450	Even in an ultralow alternate magnetic field, nanoparticles generated sufficient heat to cause tumor death.					
30711662	14	13	theme	cells	2535:2539	arg1	viability					2492:2500	the viability	2488:2500	the viability of osteosarcoma and glioblastoma cells	2488:2539	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	4	14	theme	microscopy	865:874	arg1	techniques					876:885	spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques	807:885	spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques	807:885	Nanoparticles were synthesized hydrothermally and characterized using a range of spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques.					
30711662	9	15	from	underexpression	1550:1564	arg1	occludin-1					1591:1600	occludin-1	1591:1600	occludin-1	1591:1600	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	9	15	from	underexpression	1550:1564	arg1	resistance					1622:1631	transepithelial resistance	1606:1631	transepithelial resistance	1606:1631	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	3	16	theme	aqueous	543:549	arg1	ferrofluid					568:577	an aqueous, surfactant-free ferrofluid	540:577	an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells	540:690	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	15	17	theme	cells	2691:2695	arg1	migration					2671:2679	the metastatic migration	2656:2679	the metastatic migration of cancer cells	2656:2695	In 3D tumor spheroids, nanoparticles greatly reduced the metastatic migration of cancer cells, while the tumor viability was reduced compared to the control group by applying magnetic hyperthermia to nanoparticle-treated spheroids.					
30711662	5	18	theme	double	892:897	arg1	coating					899:905	The double coating	888:905	The double coating on SPIONs	888:915	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	9	19	from	junctions	1539:1547	arg1	underexpression					1550:1564	underexpression	1550:1564	underexpression of ZO-1	1550:1572	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	9	19	from	junctions	1539:1547	arg1	effect					1581:1586	no effect	1578:1586	no effect on occludin-1 and transepithelial resistance	1578:1631	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	9	19	from	junctions	1539:1547	arg1	mislocalization					1497:1511	mislocalization	1497:1511	mislocalization of claudin-1 at the tight junctions	1497:1547	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	13	20	theme	solid	2187:2191	arg1	tumors					2193:2198	solid tumors	2187:2198	solid tumors	2187:2198	They are also effective against solid tumors in vitro, can cross the BBB in Drosophila, and are nontoxic based on the developmental studies of flies raised in ferrofluid-infused media.					
30711662	2	21	theme	metastasis	325:334	arg1	site					317:320	a common site	308:320	a common site of metastasis	308:334	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	2	21	theme	metastasis	325:334	arg1	bone					300:303	bone	300:303	bone	300:303	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	2	21	theme	metastasis	325:334	arg1	metastasis					325:334	metastasis	325:334	metastasis	325:334	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	9	22	theme	claudin-1	1516:1524	arg1	underexpression					1550:1564	underexpression	1550:1564	underexpression of ZO-1	1550:1572	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	9	22	theme	claudin-1	1516:1524	arg1	effect					1581:1586	no effect	1578:1586	no effect on occludin-1 and transepithelial resistance	1578:1631	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	9	22	theme	claudin-1	1516:1524	arg1	mislocalization					1497:1511	mislocalization	1497:1511	mislocalization of claudin-1 at the tight junctions	1497:1547	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	17	23	theme	composite	2994:3002	arg1	nanoparticles					3004:3016	These composite nanoparticles	2988:3016	These composite nanoparticles	2988:3016	These composite nanoparticles show great promise as an anticancer biomaterial for the treatment of different types of cancer and may serve as an alternative or addendum to traditional chemotherapies.					
30711662	15	24	theme	nanoparticle-treated	2803:2822	arg1	spheroids					2824:2832	nanoparticle-treated spheroids	2803:2832	nanoparticle-treated spheroids	2803:2832	In 3D tumor spheroids, nanoparticles greatly reduced the metastatic migration of cancer cells, while the tumor viability was reduced compared to the control group by applying magnetic hyperthermia to nanoparticle-treated spheroids.					
30711662	16	25	theme	in	2853:2854	arg1	models					2861:2866	Both in vitro and in vivo models	2835:2866	Both in vitro and in vivo models of the blood-brain barrier	2835:2893	Both in vitro and in vivo models of the blood-brain barrier evidence the ability of nanoparticles to cross the barrier and localize to the brain tissue.					
30711662	10	26	theme	basolateral	1669:1679	arg1	compartments					1681:1692	the basolateral compartments	1665:1692	the basolateral compartments	1665:1692	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	5	27	theme	physicochemical	938:952	arg1	properties					969:978	physicochemical and biological properties	938:978	physicochemical and biological properties	938:978	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	7	28	theme	uptake	1271:1276	arg1	rate					1263:1266	a higher rate	1254:1266	a higher rate of uptake by these cells	1254:1291	They showed a greater preference for cancer cells because of a higher rate of uptake by these cells and a pronounced adherence to cancer cell membrane.					
30711662	7	28	theme	uptake	1271:1276	arg1	adherence					1310:1318	a pronounced adherence	1297:1318	a pronounced adherence to cancer cell membrane	1297:1342	They showed a greater preference for cancer cells because of a higher rate of uptake by these cells and a pronounced adherence to cancer cell membrane.					
30711662	3	29	theme	iron	608:611	arg1	SPIONs					634:639	SPIONs	634:639	SPIONs	634:639	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	3	29	theme	iron	608:611	arg1	nanoparticles					619:631	superparamagnetic iron oxide nanoparticles	590:631	superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells	590:690	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	9	30	theme	ZO-1	1569:1572	arg1	underexpression					1550:1564	underexpression	1550:1564	underexpression of ZO-1	1550:1572	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	9	30	theme	ZO-1	1569:1572	arg1	effect					1581:1586	no effect	1578:1586	no effect on occludin-1 and transepithelial resistance	1578:1631	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	9	30	theme	ZO-1	1569:1572	arg1	mislocalization					1497:1511	mislocalization	1497:1511	mislocalization of claudin-1 at the tight junctions	1497:1547	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	8	31	theme	ultralow	1356:1363	arg1	field					1384:1388	an ultralow alternate magnetic field	1353:1388	an ultralow alternate magnetic field	1353:1388	Even in an ultralow alternate magnetic field, nanoparticles generated sufficient heat to cause tumor death.					
30711662	0	32	theme	superparamagnetic	60:76	arg1	earthicles					118:127	earthicles	118:127	earthicles	118:127	Brain and bone cancer targeting by a ferrofluid composed of superparamagnetic iron-oxide/silica/carbon nanoparticles (earthicles).					
30711662	0	32	theme	superparamagnetic	60:76	arg1	nanoparticles					103:115	superparamagnetic iron-oxide/silica/carbon nanoparticles	60:115	superparamagnetic iron-oxide/silica/carbon nanoparticles (earthicles)	60:128	Brain and bone cancer targeting by a ferrofluid composed of superparamagnetic iron-oxide/silica/carbon nanoparticles (earthicles).					
30711662	15	33	theme	3D	2606:2607	arg1	spheroids					2615:2623	3D tumor spheroids	2606:2623	3D tumor spheroids	2606:2623	In 3D tumor spheroids, nanoparticles greatly reduced the metastatic migration of cancer cells, while the tumor viability was reduced compared to the control group by applying magnetic hyperthermia to nanoparticle-treated spheroids.					
30711662	3	34	dep	aqueous	543:549	arg1	surfactant-free					552:566	surfactant-free	552:566	surfactant-free	552:566	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	9	35	from	effect	1581:1586	arg1	occludin-1					1591:1600	occludin-1	1591:1600	occludin-1	1591:1600	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	9	35	from	effect	1581:1586	arg1	resistance					1622:1631	transepithelial resistance	1606:1631	transepithelial resistance	1606:1631	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	14	36	from	effects	2572:2578	arg1	lines					2596:2600	primary cell lines	2583:2600	primary cell lines	2583:2600	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	8	37	theme	magnetic	1375:1382	arg1	field					1384:1388	an ultralow alternate magnetic field	1353:1388	an ultralow alternate magnetic field	1353:1388	Even in an ultralow alternate magnetic field, nanoparticles generated sufficient heat to cause tumor death.					
30711662	17	38	theme	types	3097:3101	arg1	treatment					3074:3082	the treatment	3070:3082	the treatment of different types of cancer	3070:3111	These composite nanoparticles show great promise as an anticancer biomaterial for the treatment of different types of cancer and may serve as an alternative or addendum to traditional chemotherapies.					
30711662	5	39	from	coating	899:905	arg1	SPIONs					910:915	SPIONs	910:915	SPIONs	910:915	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	5	40	theme	colloidal	991:999	arg1	stability					1001:1009	colloidal stability	991:1009	colloidal stability	991:1009	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	6	41	theme	tumors	1123:1128	arg1	viability					1070:1078	the viability	1066:1078	the viability of glioblastoma and osteosarcoma cells and tumors	1066:1128	Nanoparticles decreased the viability of glioblastoma and osteosarcoma cells and tumors more than that of their primary and non-transformed analogues.					
30711662	10	42	theme	LAMP1	1713:1717	arg1	examination					1698:1708	examination	1698:1708	examination of LAMP1	1698:1717	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	7	43	theme	cancer	1230:1235	arg1	cells					1237:1241	cancer cells	1230:1241	cancer cells	1230:1241	They showed a greater preference for cancer cells because of a higher rate of uptake by these cells and a pronounced adherence to cancer cell membrane.					
30711662	5	44	theme	biological	958:967	arg1	properties					969:978	physicochemical and biological properties	938:978	physicochemical and biological properties	938:978	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	6	45	theme	cells	1113:1117	arg1	viability					1070:1078	the viability	1066:1078	the viability of glioblastoma and osteosarcoma cells and tumors	1066:1128	Nanoparticles decreased the viability of glioblastoma and osteosarcoma cells and tumors more than that of their primary and non-transformed analogues.					
30711662	2	46	theme	healthy	400:406	arg1	milieu					417:422	its healthy cellular milieu	396:422	its healthy cellular milieu	396:422	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	13	47	theme	developmental	2273:2285	arg1	studies					2287:2293	the developmental studies	2269:2293	the developmental studies of flies raised in ferrofluid-infused media	2269:2337	They are also effective against solid tumors in vitro, can cross the BBB in Drosophila, and are nontoxic based on the developmental studies of flies raised in ferrofluid-infused media.					
30711662	6	48	theme	glioblastoma	1083:1094	arg1	cells					1113:1117	glioblastoma and osteosarcoma cells	1083:1117	cells	1113:1117	Nanoparticles decreased the viability of glioblastoma and osteosarcoma cells and tumors more than that of their primary and non-transformed analogues.					
30711662	17	49	theme	great	3023:3027	arg1	biomaterial					3054:3064	biomaterial	3054:3064	biomaterial	3054:3064	These composite nanoparticles show great promise as an anticancer biomaterial for the treatment of different types of cancer and may serve as an alternative or addendum to traditional chemotherapies.					
30711662	17	49	theme	great	3023:3027	arg1	promise					3029:3035	great promise	3023:3035	great promise	3023:3035	These composite nanoparticles show great promise as an anticancer biomaterial for the treatment of different types of cancer and may serve as an alternative or addendum to traditional chemotherapies.					
30711662	9	50	from	Nanoparticles	1453:1465	arg1	model					1484:1488	MDCK-MDR1 BBB model	1470:1488	MDCK-MDR1 BBB model	1470:1488	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	7	51	theme	cell	1330:1333	arg1	membrane					1335:1342	cancer cell membrane	1323:1342	cancer cell membrane	1323:1342	They showed a greater preference for cancer cells because of a higher rate of uptake by these cells and a pronounced adherence to cancer cell membrane.					
30711662	15	52	theme	magnetic	2778:2785	arg1	hyperthermia					2787:2798	magnetic hyperthermia	2778:2798	magnetic hyperthermia	2778:2798	In 3D tumor spheroids, nanoparticles greatly reduced the metastatic migration of cancer cells, while the tumor viability was reduced compared to the control group by applying magnetic hyperthermia to nanoparticle-treated spheroids.					
30711662	6	53	theme	non-transformed	1166:1180	arg1	analogues					1182:1190	their primary and non-transformed analogues	1148:1190	their primary and non-transformed analogues	1148:1190	Nanoparticles decreased the viability of glioblastoma and osteosarcoma cells and tumors more than that of their primary and non-transformed analogues.					
30711662	13	54	from	BBB	2224:2226	arg1	Drosophila					2231:2240	Drosophila	2231:2240	Drosophila	2231:2240	They are also effective against solid tumors in vitro, can cross the BBB in Drosophila, and are nontoxic based on the developmental studies of flies raised in ferrofluid-infused media.					
30711662	2	55	theme	common	310:315	arg1	site					317:320	a common site	308:320	a common site of metastasis	308:334	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	2	55	theme	common	310:315	arg1	bone					300:303	bone	300:303	bone	300:303	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	2	55	theme	common	310:315	arg1	metastasis					325:334	metastasis	325:334	metastasis	325:334	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	14	56	theme	primary	2583:2589	arg1	lines					2596:2600	primary cell lines	2583:2600	primary cell lines	2583:2600	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	5	57	theme	targeting	1022:1030	arg1	efficacy					1032:1039	cancer targeting efficacy	1015:1039	cancer targeting efficacy	1015:1039	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	6	58	theme	primary	1154:1160	arg1	analogues					1182:1190	their primary and non-transformed analogues	1148:1190	their primary and non-transformed analogues	1148:1190	Nanoparticles decreased the viability of glioblastoma and osteosarcoma cells and tumors more than that of their primary and non-transformed analogues.					
30711662	3	59	theme	silicate	654:661	arg1	mesolayers					663:672	silicate mesolayers	654:672	silicate mesolayers	654:672	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	12	60	theme	greater	2092:2098	arg1	toxicity					2100:2107	a greater toxicity	2090:2107	a greater toxicity in these cells compared to the primary cells	2090:2152	In conclusion, these nanoparticulate ferrofluids preferentially bind to cancer cells and, hence, exhibit a greater toxicity in these cells compared to the primary cells.					
30711662	0	61	dep	Brain	0:4	arg1	cancer					15:20	cancer	15:20	cancer	15:20	Brain and bone cancer targeting by a ferrofluid composed of superparamagnetic iron-oxide/silica/carbon nanoparticles (earthicles).					
30711662	13	62	theme	flies	2298:2302	arg1	studies					2287:2293	the developmental studies	2269:2293	the developmental studies of flies raised in ferrofluid-infused media	2269:2337	They are also effective against solid tumors in vitro, can cross the BBB in Drosophila, and are nontoxic based on the developmental studies of flies raised in ferrofluid-infused media.					
30711662	7	63	theme	higher	1256:1261	arg1	rate					1263:1266	a higher rate	1254:1266	a higher rate of uptake by these cells	1254:1291	They showed a greater preference for cancer cells because of a higher rate of uptake by these cells and a pronounced adherence to cancer cell membrane.					
30711662	11	64	theme	adverse	1944:1950	arg1	effects					1961:1967	no adverse systemic effects	1941:1967	no adverse systemic effects	1941:1967	The passage was noninvasive and caused no adverse systemic effects to the animals.					
30711662	12	65	from	toxicity	2100:2107	arg1	cells					2118:2122	these cells	2112:2122	these cells	2112:2122	In conclusion, these nanoparticulate ferrofluids preferentially bind to cancer cells and, hence, exhibit a greater toxicity in these cells compared to the primary cells.					
30711662	4	66	theme	techniques	876:885	arg1	range					798:802	a range	796:802	a range of spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques	796:885	Nanoparticles were synthesized hydrothermally and characterized using a range of spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques.					
30711662	14	67	dep	novel	2389:2393	arg1	synthesized					2411:2421	synthesized	2411:2421	synthesized	2411:2421	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	17	68	theme	different	3087:3095	arg1	types					3097:3101	different types	3087:3101	different types of cancer	3087:3111	These composite nanoparticles show great promise as an anticancer biomaterial for the treatment of different types of cancer and may serve as an alternative or addendum to traditional chemotherapies.					
30711662	1	69	theme	tumors	260:265	arg1	barrier					219:225	the blood-brain barrier	203:225	the blood-brain barrier (BBB)	203:231	Despite the advances in molecularly targeted therapies, delivery across the blood-brain barrier (BBB) and the targeting of brain tumors remains a challenge.					
30711662	1	69	theme	tumors	260:265	arg1	targeting					241:249	the targeting	237:249	the targeting of brain tumors	237:265	Despite the advances in molecularly targeted therapies, delivery across the blood-brain barrier (BBB) and the targeting of brain tumors remains a challenge.					
30711662	1	69	theme	tumors	260:265	arg1	BBB					228:230	BBB	228:230	BBB	228:230	Despite the advances in molecularly targeted therapies, delivery across the blood-brain barrier (BBB) and the targeting of brain tumors remains a challenge.					
30711662	16	70	theme	blood-brain	2875:2885	arg1	barrier					2887:2893	the blood-brain barrier	2871:2893	the blood-brain barrier	2871:2893	Both in vitro and in vivo models of the blood-brain barrier evidence the ability of nanoparticles to cross the barrier and localize to the brain tissue.					
30711662	17	71	theme	traditional	3160:3170	arg1	chemotherapies					3172:3185	traditional chemotherapies	3160:3185	traditional chemotherapies	3160:3185	These composite nanoparticles show great promise as an anticancer biomaterial for the treatment of different types of cancer and may serve as an alternative or addendum to traditional chemotherapies.					
30711662	14	72	theme	nanoparticle-based	2433:2450	arg1	ferrofluid					2452:2461	a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid	2387:2461	a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid	2387:2461	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	14	72	theme	nanoparticle-based	2433:2450	arg1	effective					2466:2474	effective	2466:2474	effective	2466:2474	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	10	73	theme	optic	1828:1832	arg1	lobes					1834:1838	the optic lobes	1824:1838	the optic lobes of the third instar	1824:1858	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	10	73	theme	optic	1828:1832	arg1	instar					1853:1858	the third instar	1843:1858	the third instar	1843:1858	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	9	74	theme	BBB	1480:1482	arg1	model					1484:1488	MDCK-MDR1 BBB model	1470:1488	MDCK-MDR1 BBB model	1470:1488	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	4	75	theme	hydrodynamic	839:850	arg1	techniques					876:885	spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques	807:885	spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques	807:885	Nanoparticles were synthesized hydrothermally and characterized using a range of spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques.					
30711662	3	76	from	13 nm	700:704	arg1	average					717:723	average	717:723	average	717:723	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	3	76	from	13 nm	700:704	arg1	size					709:712	size	709:712	size	709:712	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	15	77	theme	control	2752:2758	arg1	group					2760:2764	the control group	2748:2764	the control group	2748:2764	In 3D tumor spheroids, nanoparticles greatly reduced the metastatic migration of cancer cells, while the tumor viability was reduced compared to the control group by applying magnetic hyperthermia to nanoparticle-treated spheroids.					
30711662	10	78	theme	larval	1860:1865	arg1	brains					1867:1872	larval brains	1860:1872	larval brains of Drosophila melanogaster	1860:1899	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	12	79	theme	cancer	2057:2062	arg1	cells					2064:2068	cancer cells	2057:2068	cancer cells	2057:2068	In conclusion, these nanoparticulate ferrofluids preferentially bind to cancer cells and, hence, exhibit a greater toxicity in these cells compared to the primary cells.					
30711662	4	80	theme	electron	856:863	arg1	microscopy					865:874	electron microscopy	856:874	electron microscopy	856:874	Nanoparticles were synthesized hydrothermally and characterized using a range of spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques.					
30711662	4	81	theme	diffractometric	822:836	arg1	techniques					876:885	spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques	807:885	spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques	807:885	Nanoparticles were synthesized hydrothermally and characterized using a range of spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques.					
30711662	10	82	theme	third	1847:1851	arg1	instar					1853:1858	the third instar	1843:1858	the third instar	1843:1858	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	1	83	theme	blood-brain	207:217	arg1	barrier					219:225	the blood-brain barrier	203:225	the blood-brain barrier (BBB)	203:231	Despite the advances in molecularly targeted therapies, delivery across the blood-brain barrier (BBB) and the targeting of brain tumors remains a challenge.					
30711662	1	83	theme	blood-brain	207:217	arg1	BBB					228:230	BBB	228:230	BBB	228:230	Despite the advances in molecularly targeted therapies, delivery across the blood-brain barrier (BBB) and the targeting of brain tumors remains a challenge.					
30711662	10	84	theme	instar	1853:1858	arg1	lobes					1834:1838	the optic lobes	1824:1838	the optic lobes of the third instar	1824:1858	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	10	84	theme	instar	1853:1858	arg1	instar					1853:1858	the third instar	1843:1858	the third instar	1843:1858	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	16	85	theme	nanoparticles	2919:2931	arg1	ability					2908:2914	the ability	2904:2914	the ability of nanoparticles to cross the barrier and localize to the brain tissue	2904:2985	Both in vitro and in vivo models of the blood-brain barrier evidence the ability of nanoparticles to cross the barrier and localize to the brain tissue.					
30711662	14	86	dep	STATEMENT	2340:2348	arg1	demonstrate					2370:2380	demonstrate	2370:2380	demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines	2370:2600	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	10	87	theme	melanogaster	1888:1899	arg1	brains					1867:1872	larval brains	1860:1872	larval brains of Drosophila melanogaster	1860:1899	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	15	88	theme	cancer	2684:2689	arg1	cells					2691:2695	cancer cells	2684:2695	cancer cells	2684:2695	In 3D tumor spheroids, nanoparticles greatly reduced the metastatic migration of cancer cells, while the tumor viability was reduced compared to the control group by applying magnetic hyperthermia to nanoparticle-treated spheroids.					
30711662	16	89	theme	in	2840:2841	arg1	models					2861:2866	Both in vitro and in vivo models	2835:2866	Both in vitro and in vivo models of the blood-brain barrier	2835:2893	Both in vitro and in vivo models of the blood-brain barrier evidence the ability of nanoparticles to cross the barrier and localize to the brain tissue.					
30711662	9	90	theme	tight	1533:1537	arg1	junctions					1539:1547	the tight junctions	1529:1547	the tight junctions	1529:1547	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	12	91	theme	primary	2140:2146	arg1	cells					2148:2152	the primary cells	2136:2152	the primary cells	2136:2152	In conclusion, these nanoparticulate ferrofluids preferentially bind to cancer cells and, hence, exhibit a greater toxicity in these cells compared to the primary cells.					
30711662	14	92	theme	novel	2389:2393	arg1	ferrofluid					2452:2461	a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid	2387:2461	a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid	2387:2461	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	14	92	theme	novel	2389:2393	arg1	effective					2466:2474	effective	2466:2474	effective	2466:2474	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	16	93	theme	brain	2974:2978	arg1	tissue					2980:2985	the brain tissue	2970:2985	the brain tissue	2970:2985	Both in vitro and in vivo models of the blood-brain barrier evidence the ability of nanoparticles to cross the barrier and localize to the brain tissue.					
30711662	16	94	dep	in	2853:2854	arg1	vivo					2856:2859	vivo	2856:2859	vivo	2856:2859	Both in vitro and in vivo models of the blood-brain barrier evidence the ability of nanoparticles to cross the barrier and localize to the brain tissue.					
30711662	3	95	theme	oxide	613:617	arg1	SPIONs					634:639	SPIONs	634:639	SPIONs	634:639	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	3	95	theme	oxide	613:617	arg1	nanoparticles					619:631	superparamagnetic iron oxide nanoparticles	590:631	superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells	590:690	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	3	96	theme	superparamagnetic	590:606	arg1	SPIONs					634:639	SPIONs	634:639	SPIONs	634:639	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	3	96	theme	superparamagnetic	590:606	arg1	nanoparticles					619:631	superparamagnetic iron oxide nanoparticles	590:631	superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells	590:690	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
30711662	2	97	from	milieu	417:422	arg1	tumor					385:389	the tumor	381:389	the tumor from its healthy cellular milieu	381:422	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	0	98	theme	iron-oxide/silica/carbon	78:101	arg1	earthicles					118:127	earthicles	118:127	earthicles	118:127	Brain and bone cancer targeting by a ferrofluid composed of superparamagnetic iron-oxide/silica/carbon nanoparticles (earthicles).					
30711662	0	98	theme	iron-oxide/silica/carbon	78:101	arg1	nanoparticles					103:115	superparamagnetic iron-oxide/silica/carbon nanoparticles	60:115	superparamagnetic iron-oxide/silica/carbon nanoparticles (earthicles)	60:128	Brain and bone cancer targeting by a ferrofluid composed of superparamagnetic iron-oxide/silica/carbon nanoparticles (earthicles).					
30711662	1	99	theme	targeted	167:174	arg1	therapies					176:184	molecularly targeted therapies	155:184	molecularly targeted therapies	155:184	Despite the advances in molecularly targeted therapies, delivery across the blood-brain barrier (BBB) and the targeting of brain tumors remains a challenge.					
30711662	12	100	dep	bind	2049:2052	arg1	exhibit					2082:2088	exhibit	2082:2088	exhibit a greater toxicity in these cells compared to the primary cells	2082:2152	In conclusion, these nanoparticulate ferrofluids preferentially bind to cancer cells and, hence, exhibit a greater toxicity in these cells compared to the primary cells.					
30711662	14	101	theme	SIGNIFICANCE	2353:2364	arg1	STATEMENT					2340:2348	STATEMENT	2340:2348	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.	2340:2601	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	12	102	theme	nanoparticulate	2006:2020	arg1	ferrofluids					2022:2032	these nanoparticulate ferrofluids	2000:2032	these nanoparticulate ferrofluids	2000:2032	In conclusion, these nanoparticulate ferrofluids preferentially bind to cancer cells and, hence, exhibit a greater toxicity in these cells compared to the primary cells.					
30711662	15	103	theme	tumor	2609:2613	arg1	spheroids					2615:2623	3D tumor spheroids	2606:2623	3D tumor spheroids	2606:2623	In 3D tumor spheroids, nanoparticles greatly reduced the metastatic migration of cancer cells, while the tumor viability was reduced compared to the control group by applying magnetic hyperthermia to nanoparticle-treated spheroids.					
30711662	8	104	theme	alternate	1365:1373	arg1	field					1384:1388	an ultralow alternate magnetic field	1353:1388	an ultralow alternate magnetic field	1353:1388	Even in an ultralow alternate magnetic field, nanoparticles generated sufficient heat to cause tumor death.					
30711662	7	105	theme	pronounced	1299:1308	arg1	adherence					1310:1318	a pronounced adherence	1297:1318	a pronounced adherence to cancer cell membrane	1297:1342	They showed a greater preference for cancer cells because of a higher rate of uptake by these cells and a pronounced adherence to cancer cell membrane.					
30711662	1	106	from	advances	143:150	arg1	therapies					176:184	molecularly targeted therapies	155:184	molecularly targeted therapies	155:184	Despite the advances in molecularly targeted therapies, delivery across the blood-brain barrier (BBB) and the targeting of brain tumors remains a challenge.					
30711662	9	107	theme	transepithelial	1606:1620	arg1	resistance					1622:1631	transepithelial resistance	1606:1631	transepithelial resistance	1606:1631	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	2	108	theme	capable	359:365	arg1	therapies					349:357	therapies	349:357	therapies capable of discerning the tumor from its healthy cellular milieu	349:422	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	7	109	theme	greater	1207:1213	arg1	preference					1215:1224	a greater preference	1205:1224	a greater preference for cancer cells	1205:1241	They showed a greater preference for cancer cells because of a higher rate of uptake by these cells and a pronounced adherence to cancer cell membrane.					
30711662	5	110	theme	properties	969:978	arg1	efficacy					1032:1039	cancer targeting efficacy	1015:1039	cancer targeting efficacy	1015:1039	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	5	110	theme	properties	969:978	arg1	number					928:933	a number	926:933	a number	926:933	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	5	110	theme	properties	969:978	arg1	stability					1001:1009	colloidal stability	991:1009	colloidal stability	991:1009	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	6	111	theme	osteosarcoma	1100:1111	arg1	cells					1113:1117	glioblastoma and osteosarcoma cells	1083:1117	cells	1113:1117	Nanoparticles decreased the viability of glioblastoma and osteosarcoma cells and tumors more than that of their primary and non-transformed analogues.					
30711662	2	112	theme	cellular	408:415	arg1	milieu					417:422	its healthy cellular milieu	396:422	its healthy cellular milieu	396:422	Like brain, bone is a common site of metastasis and requires therapies capable of discerning the tumor from its healthy cellular milieu.					
30711662	17	113	dep	alternative	3133:3143	arg1	an					3130:3131	an	3130:3131	an	3130:3131	These composite nanoparticles show great promise as an anticancer biomaterial for the treatment of different types of cancer and may serve as an alternative or addendum to traditional chemotherapies.					
30711662	7	114	theme	cancer	1323:1328	arg1	membrane					1335:1342	cancer cell membrane	1323:1342	cancer cell membrane	1323:1342	They showed a greater preference for cancer cells because of a higher rate of uptake by these cells and a pronounced adherence to cancer cell membrane.					
30711662	4	115	theme	spectroscopic	807:819	arg1	techniques					876:885	spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques	807:885	spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques	807:885	Nanoparticles were synthesized hydrothermally and characterized using a range of spectroscopic, diffractometric, hydrodynamic and electron microscopy techniques.					
30711662	14	116	theme	glioblastoma	2522:2533	arg1	cells					2535:2539	osteosarcoma and glioblastoma cells	2505:2539	cells	2535:2539	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	8	117	theme	tumor	1440:1444	arg1	death					1446:1450	tumor death	1440:1450	tumor death	1440:1450	Even in an ultralow alternate magnetic field, nanoparticles generated sufficient heat to cause tumor death.					
30711662	14	118	theme	osteosarcoma	2505:2516	arg1	cells					2535:2539	osteosarcoma and glioblastoma cells	2505:2539	cells	2535:2539	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	15	119	theme	metastatic	2660:2669	arg1	migration					2671:2679	the metastatic migration	2656:2679	the metastatic migration of cancer cells	2656:2695	In 3D tumor spheroids, nanoparticles greatly reduced the metastatic migration of cancer cells, while the tumor viability was reduced compared to the control group by applying magnetic hyperthermia to nanoparticle-treated spheroids.					
30711662	16	120	dep	in	2840:2841	arg1	vitro					2843:2847	vitro	2843:2847	vitro	2843:2847	Both in vitro and in vivo models of the blood-brain barrier evidence the ability of nanoparticles to cross the barrier and localize to the brain tissue.					
30711662	14	121	theme	cell	2591:2594	arg1	lines					2596:2600	primary cell lines	2583:2600	primary cell lines	2583:2600	STATEMENT OF SIGNIFICANCE: We demonstrate that a novel, hydrothermally synthesized composite nanoparticle-based ferrofluid is effective in reducing the viability of osteosarcoma and glioblastoma cells in vitro, while having minimal effects on primary cell lines.					
30711662	5	122	theme	cancer	1015:1020	arg1	efficacy					1032:1039	cancer targeting efficacy	1015:1039	cancer targeting efficacy	1015:1039	The double coating on SPIONs affected a number of physicochemical and biological properties, including colloidal stability and cancer targeting efficacy.					
30711662	9	123	from	mislocalization	1497:1511	arg1	occludin-1					1591:1600	occludin-1	1591:1600	occludin-1	1591:1600	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	9	123	from	mislocalization	1497:1511	arg1	resistance					1622:1631	transepithelial resistance	1606:1631	transepithelial resistance	1606:1631	Nanoparticles in MDCK-MDR1 BBB model caused mislocalization of claudin-1 at the tight junctions, underexpression of ZO-1 and no effect on occludin-1 and transepithelial resistance.					
30711662	10	124	located	detected	1653:1660	arg2	Nanoparticles					1634:1646	Nanoparticles	1634:1646	Nanoparticles	1634:1646	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	10	124	located	detected	1653:1660	arg1	compartments					1681:1692	the basolateral compartments	1665:1692	the basolateral compartments	1665:1692	Nanoparticles were detected in the basolateral compartments and examination of LAMP1 demonstrated that nanoparticles escaped the lysosome, traversed the BBB transcellularly and localized to the optic lobes of the third instar larval brains of Drosophila melanogaster.					
30711662	3	125	theme	proposed	491:498	arg1	concept					500:506	the previously proposed concept	476:506	the previously proposed concept of the earthicle	476:523	To tackle these challenges, we made a variation on the previously proposed concept of the earthicle and fabricated an aqueous, surfactant-free ferrofluid containing superparamagnetic iron oxide nanoparticles (SPIONs) coated with silicate mesolayers and carbon shells, having 13 nm in size on average.					
31815604	8	0	theme	many	1065:1068	arg1	surfaces					1070:1077	many surfaces	1065:1077	many surfaces of the substrates	1065:1095	The results showed good adhesion property of the CS-DPA/γPGA-DA adhesive on many surfaces of the substrates.					
31815604	11	1	dep	In	1529:1530	arg1	vitro					1532:1536	vitro	1532:1536	vitro	1532:1536	In vitro experiments demonstrated that the adhesive had good biocompatibility.					
31815604	10	2	theme	catechol	1444:1451	arg1	groups					1453:1458	the catechol groups	1440:1458	the catechol groups	1440:1458	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	11	3	theme	good	1585:1588	arg1	biocompatibility					1590:1605	good biocompatibility	1585:1605	good biocompatibility	1585:1605	In vitro experiments demonstrated that the adhesive had good biocompatibility.					
31815604	10	4	theme	CS	1507:1508	arg1	polymers					1519:1526	the CS and γPGA polymers	1503:1526	the CS and γPGA polymers	1503:1526	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	8	5	theme	good	1008:1011	arg1	property					1022:1029	good adhesion property	1008:1029	good adhesion property of the CS-DPA/γPGA-DA adhesive on many surfaces of the substrates	1008:1095	The results showed good adhesion property of the CS-DPA/γPGA-DA adhesive on many surfaces of the substrates.					
31815604	2	6	theme	adhesive	167:174	arg1	properties					176:185	the adhesive properties	163:185	the adhesive properties	163:185	However, the adhesive properties are still insufficient to meet the practical applications.					
31815604	2	6	theme	adhesive	167:174	arg1	insufficient					197:208	insufficient	197:208	insufficient	197:208	However, the adhesive properties are still insufficient to meet the practical applications.					
31815604	7	7	theme	degrees	899:905	arg1	Effects					872:878	Effects	872:878	Effects of the substituted degrees and substrates on the adhesion strength	872:945	Effects of the substituted degrees and substrates on the adhesion strength were measured by tensile testing machine.					
31815604	10	8	theme	adhesion	1251:1258	arg1	due					1278:1280	due	1278:1280	due	1278:1280	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	10	8	theme	adhesion	1251:1258	arg1	property					1260:1267	The high adhesion property	1242:1267	The high adhesion property	1242:1267	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	7	9	theme	testing	972:978	arg1	machine					980:986	tensile testing machine	964:986	tensile testing machine	964:986	Effects of the substituted degrees and substrates on the adhesion strength were measured by tensile testing machine.					
31815604	9	10	theme	tissue	1224:1229	arg1	adhesives					1231:1239	commercially available tissue adhesives	1201:1239	commercially available tissue adhesives	1201:1239	Especially on the arthrodial cartilage, the adhesive strength reached around 150 kPa, much higher than commercially available tissue adhesives.					
31815604	10	11	theme	high	1246:1249	arg1	due					1278:1280	due	1278:1280	due	1278:1280	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	10	11	theme	high	1246:1249	arg1	property					1260:1267	The high adhesion property	1242:1267	The high adhesion property	1242:1267	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	5	12	theme	catechol	744:751	arg1	compositions					753:764	the catechol compositions	740:764	the catechol compositions	740:764	The substituted degrees of the catechol groups were controlled by the catechol compositions.					
31815604	5	13	theme	substituted	678:688	arg1	degrees					690:696	The substituted degrees	674:696	The substituted degrees of the catechol groups	674:719	The substituted degrees of the catechol groups were controlled by the catechol compositions.					
31815604	9	14	theme	adhesive	1142:1149	arg1	strength					1151:1158	the adhesive strength	1138:1158	the adhesive strength	1138:1158	Especially on the arthrodial cartilage, the adhesive strength reached around 150 kPa, much higher than commercially available tissue adhesives.					
31815604	3	15	theme	efficient	383:391	arg1	way					393:395	an efficient way	380:395	an efficient way to increase cohesion force and further improve the adhesive properties	380:466	Introducing functional groups into the polymer chains that have multi-interactions among inter/intra-molecules and with substrates is an efficient way to increase cohesion force and further improve the adhesive properties.					
31815604	8	16	theme	substrates	1086:1095	arg1	surfaces					1070:1077	many surfaces	1065:1077	many surfaces of the substrates	1065:1095	The results showed good adhesion property of the CS-DPA/γPGA-DA adhesive on many surfaces of the substrates.					
31815604	8	17	theme	adhesion	1013:1020	arg1	property					1022:1029	good adhesion property	1008:1029	good adhesion property of the CS-DPA/γPGA-DA adhesive on many surfaces of the substrates	1008:1095	The results showed good adhesion property of the CS-DPA/γPGA-DA adhesive on many surfaces of the substrates.					
31815604	4	18	contain	containing	543:552	arg1	DPA					520:522	DPA	520:522	DPA	520:522	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	4	18	contain	containing	543:552	arg2	groups					583:588	adhesion functional catechol groups	554:588	adhesion functional catechol groups	554:588	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	4	18	contain	containing	543:552	arg1	acid					514:517	3,4-dihydroxyphenyl propionic acid	484:517	3,4-dihydroxyphenyl propionic acid (DPA)	484:523	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	7	19	theme	substituted	887:897	arg1	degrees					899:905	degrees	899:905	degrees	899:905	Effects of the substituted degrees and substrates on the adhesion strength were measured by tensile testing machine.					
31815604	3	20	with	multi-interactions	310:327	arg1	substrates					366:375	substrates	366:375	substrates	366:375	Introducing functional groups into the polymer chains that have multi-interactions among inter/intra-molecules and with substrates is an efficient way to increase cohesion force and further improve the adhesive properties.					
31815604	7	21	theme	tensile	964:970	arg1	machine					980:986	tensile testing machine	964:986	tensile testing machine	964:986	Effects of the substituted degrees and substrates on the adhesion strength were measured by tensile testing machine.					
31815604	9	22	theme	arthrodial	1116:1125	arg1	cartilage					1127:1135	the arthrodial cartilage	1112:1135	the arthrodial cartilage	1112:1135	Especially on the arthrodial cartilage, the adhesive strength reached around 150 kPa, much higher than commercially available tissue adhesives.					
31815604	12	23	theme	suitable	1670:1677	arg1	biomaterial					1693:1703	a very suitable and promising biomaterial	1663:1703	a very suitable and promising biomaterial in the clinical medicine field	1663:1734	These results suggested the catechol-based adhesive is a very suitable and promising biomaterial in the clinical medicine field.					
31815604	3	24	theme	functional	258:267	arg1	groups					269:274	functional groups	258:274	functional groups	258:274	Introducing functional groups into the polymer chains that have multi-interactions among inter/intra-molecules and with substrates is an efficient way to increase cohesion force and further improve the adhesive properties.					
31815604	6	25	theme	CS-DPA/γPGA-DA	807:820	arg1	adhesives					823:831	chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives	780:831	chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives prepared by mixing CS-DPA and γPGA-DA	780:869	DPA modified chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives prepared by mixing CS-DPA and γPGA-DA.					
31815604	10	26	dep	interactions	1411:1422	arg1	formation					1424:1432	formation	1424:1432	formation among the catechol groups	1424:1458	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	11	27	contain	had	1581:1583	arg1	adhesive					1572:1579	adhesive	1572:1579	adhesive	1572:1579	In vitro experiments demonstrated that the adhesive had good biocompatibility.					
31815604	11	27	contain	had	1581:1583	arg2	biocompatibility					1590:1605	good biocompatibility	1585:1605	good biocompatibility	1585:1605	In vitro experiments demonstrated that the adhesive had good biocompatibility.					
31815604	10	28	theme	γPGA	1514:1517	arg1	polymers					1519:1526	the CS and γPGA polymers	1503:1526	the CS and γPGA polymers	1503:1526	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	8	29	theme	adhesive	1053:1060	arg1	property					1022:1029	good adhesion property	1008:1029	good adhesion property of the CS-DPA/γPGA-DA adhesive on many surfaces of the substrates	1008:1095	The results showed good adhesion property of the CS-DPA/γPGA-DA adhesive on many surfaces of the substrates.					
31815604	10	30	theme	high	1362:1365	arg1	forces					1376:1381	the high cohesion forces	1358:1381	the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers	1358:1526	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	3	31	theme	cohesion	409:416	arg1	force					418:422	cohesion force	409:422	cohesion force	409:422	Introducing functional groups into the polymer chains that have multi-interactions among inter/intra-molecules and with substrates is an efficient way to increase cohesion force and further improve the adhesive properties.					
31815604	5	32	theme	groups	714:719	arg1	degrees					690:696	The substituted degrees	674:696	The substituted degrees of the catechol groups	674:719	The substituted degrees of the catechol groups were controlled by the catechol compositions.					
31815604	6	33	theme	γPGA	801:804	arg1	adhesives					823:831	chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives	780:831	chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives prepared by mixing CS-DPA and γPGA-DA	780:869	DPA modified chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives prepared by mixing CS-DPA and γPGA-DA.					
31815604	9	34	theme	available	1214:1222	arg1	adhesives					1231:1239	commercially available tissue adhesives	1201:1239	commercially available tissue adhesives	1201:1239	Especially on the arthrodial cartilage, the adhesive strength reached around 150 kPa, much higher than commercially available tissue adhesives.					
31815604	12	35	from	biomaterial	1693:1703	arg1	field					1730:1734	the clinical medicine field	1708:1734	the clinical medicine field	1708:1734	These results suggested the catechol-based adhesive is a very suitable and promising biomaterial in the clinical medicine field.					
31815604	4	36	theme	3,4-dihydroxyphenyl	484:502	arg1	DPA					520:522	DPA	520:522	DPA	520:522	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	4	36	theme	3,4-dihydroxyphenyl	484:502	arg1	acid					514:517	3,4-dihydroxyphenyl propionic acid	484:517	3,4-dihydroxyphenyl propionic acid (DPA)	484:523	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	3	37	contain	have	305:308	arg1	chains					293:298	the polymer chains	281:298	the polymer chains that have multi-interactions among inter/intra-molecules and with substrates	281:375	Introducing functional groups into the polymer chains that have multi-interactions among inter/intra-molecules and with substrates is an efficient way to increase cohesion force and further improve the adhesive properties.					
31815604	3	37	contain	have	305:308	arg2	multi-interactions					310:327	multi-interactions	310:327	multi-interactions among inter/intra-molecules and with substrates	310:375	Introducing functional groups into the polymer chains that have multi-interactions among inter/intra-molecules and with substrates is an efficient way to increase cohesion force and further improve the adhesive properties.					
31815604	10	38	theme	electrostatic	1468:1480	arg1	interactions					1482:1493	the electrostatic interactions	1464:1493	the electrostatic interactions between the CS and γPGA polymers	1464:1526	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	3	39	theme	adhesive	448:455	arg1	properties					457:466	the adhesive properties	444:466	the adhesive properties	444:466	Introducing functional groups into the polymer chains that have multi-interactions among inter/intra-molecules and with substrates is an efficient way to increase cohesion force and further improve the adhesive properties.					
31815604	3	40	theme	polymer	285:291	arg1	chains					293:298	the polymer chains	281:298	the polymer chains that have multi-interactions among inter/intra-molecules and with substrates	281:375	Introducing functional groups into the polymer chains that have multi-interactions among inter/intra-molecules and with substrates is an efficient way to increase cohesion force and further improve the adhesive properties.					
31815604	10	41	theme	adhesion	1289:1296	arg1	interactions					1298:1309	the adhesion interactions	1285:1309	the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers	1285:1526	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	12	42	theme	medicine	1721:1728	arg1	field					1730:1734	the clinical medicine field	1708:1734	the clinical medicine field	1708:1734	These results suggested the catechol-based adhesive is a very suitable and promising biomaterial in the clinical medicine field.					
31815604	4	43	theme	adhesion	554:561	arg1	groups					583:588	adhesion functional catechol groups	554:588	adhesion functional catechol groups	554:588	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	6	44	theme	modified	792:799	arg1	adhesives					823:831	chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives	780:831	chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives prepared by mixing CS-DPA and γPGA-DA	780:869	DPA modified chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives prepared by mixing CS-DPA and γPGA-DA.					
31815604	12	45	theme	clinical	1712:1719	arg1	field					1730:1734	the clinical medicine field	1708:1734	the clinical medicine field	1708:1734	These results suggested the catechol-based adhesive is a very suitable and promising biomaterial in the clinical medicine field.					
31815604	4	46	theme	propionic	504:512	arg1	DPA					520:522	DPA	520:522	DPA	520:522	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	4	46	theme	propionic	504:512	arg1	acid					514:517	3,4-dihydroxyphenyl propionic acid	484:517	3,4-dihydroxyphenyl propionic acid (DPA)	484:523	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	0	47	with	bio-adhesive	16:27	arg1	multi-interactions					34:51	multi-interactions	34:51	multi-interactions	34:51	Mussel inspired bio-adhesive with multi-interactions for tissue repair.					
31815604	8	48	from	property	1022:1029	arg1	surfaces					1070:1077	many surfaces	1065:1077	many surfaces of the substrates	1065:1095	The results showed good adhesion property of the CS-DPA/γPGA-DA adhesive on many surfaces of the substrates.					
31815604	0	49	theme	tissue	57:62	arg1	repair					64:69	tissue repair	57:69	tissue repair	57:69	Mussel inspired bio-adhesive with multi-interactions for tissue repair.					
31815604	4	50	theme	catechol	574:581	arg1	groups					583:588	adhesion functional catechol groups	554:588	adhesion functional catechol groups	554:588	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	12	51	dep	adhesive	1651:1658	arg1	biomaterial					1693:1703	a very suitable and promising biomaterial	1663:1703	a very suitable and promising biomaterial in the clinical medicine field	1663:1734	These results suggested the catechol-based adhesive is a very suitable and promising biomaterial in the clinical medicine field.					
31815604	2	52	theme	practical	222:230	arg1	applications					232:243	the practical applications	218:243	the practical applications	218:243	However, the adhesive properties are still insufficient to meet the practical applications.					
31815604	4	53	theme	functional	563:572	arg1	groups					583:588	adhesion functional catechol groups	554:588	adhesion functional catechol groups	554:588	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	7	54	from	Effects	872:878	arg1	strength					938:945	the adhesion strength	925:945	the adhesion strength	925:945	Effects of the substituted degrees and substrates on the adhesion strength were measured by tensile testing machine.					
31815604	10	55	theme	cohesion	1367:1374	arg1	forces					1376:1381	the high cohesion forces	1358:1381	the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers	1358:1526	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	1	56	theme	tissue	139:144	arg1	repair					146:151	tissue repair	139:151	tissue repair	139:151	Bio-adhesives based on biopolymers have been widely researched for tissue repair.					
31815604	6	57	theme	chitosan/DA	780:790	arg1	adhesives					823:831	chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives	780:831	chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives prepared by mixing CS-DPA and γPGA-DA	780:869	DPA modified chitosan/DA modified γPGA (CS-DPA/γPGA-DA) adhesives prepared by mixing CS-DPA and γPGA-DA.					
31815604	5	58	theme	catechol	705:712	arg1	groups					714:719	the catechol groups	701:719	the catechol groups	701:719	The substituted degrees of the catechol groups were controlled by the catechol compositions.					
31815604	11	59	theme	In	1529:1530	arg1	experiments					1538:1548	In vitro experiments	1529:1548	In vitro experiments	1529:1548	In vitro experiments demonstrated that the adhesive had good biocompatibility.					
31815604	10	60	theme	catechol	1323:1330	arg1	groups					1332:1337	the catechol groups	1319:1337	the catechol groups	1319:1337	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	12	61	theme	promising	1683:1691	arg1	biomaterial					1693:1703	a very suitable and promising biomaterial	1663:1703	a very suitable and promising biomaterial in the clinical medicine field	1663:1734	These results suggested the catechol-based adhesive is a very suitable and promising biomaterial in the clinical medicine field.					
31815604	7	62	theme	adhesion	929:936	arg1	strength					938:945	the adhesion strength	925:945	the adhesion strength	925:945	Effects of the substituted degrees and substrates on the adhesion strength were measured by tensile testing machine.					
31815604	10	63	theme	crosslinking	1398:1409	arg1	interactions					1411:1422	the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers	1394:1526	interactions	1411:1422	The high adhesion property might be due to the adhesion interactions between the catechol groups and substrates and the high cohesion forces induced by the crosslinking interactions formation among the catechol groups and the electrostatic interactions between the CS and γPGA polymers.					
31815604	7	64	theme	substrates	911:920	arg1	Effects					872:878	Effects	872:878	Effects of the substituted degrees and substrates on the adhesion strength	872:945	Effects of the substituted degrees and substrates on the adhesion strength were measured by tensile testing machine.					
31815604	4	65	theme	γ-polyglutamic	632:645	arg1	γPGA					653:656	γPGA	653:656	γPGA	653:656	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
31815604	4	65	theme	γ-polyglutamic	632:645	arg1	acid					647:650	γ-polyglutamic acid	632:650	γ-polyglutamic acid (γPGA)	632:657	In this study, 3,4-dihydroxyphenyl propionic acid (DPA) and dopamine (DA) containing adhesion functional catechol groups were employed to modify chitosan (CS) and γ-polyglutamic acid (γPGA), respectively.					
29502820	0	0	theme	sodium	94:99	arg1	beads					135:139	sodium alginate/graphene oxide composite beads	94:139	sodium alginate/graphene oxide composite beads	94:139	Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads.					
29502820	4	1	theme	optimal	589:595	arg1	conditions					597:606	optimal conditions	589:606	optimal conditions	589:606	The activity of the immobilized pectinase prepared under optimal conditions reached 1236.86 ± 40.21 U/g, with an enzyme activity recovery of 83.5%.					
29502820	8	2	theme	initial	1103:1109	arg1	activity					1111:1118	initial activity	1103:1118	initial activity	1103:1118	Reusability studies indicated that the immobilized pectinase retained 73% of initial activity after six times cycles.					
29502820	8	3	theme	Reusability	1026:1036	arg1	studies					1038:1044	Reusability studies	1026:1044	Reusability studies	1026:1044	Reusability studies indicated that the immobilized pectinase retained 73% of initial activity after six times cycles.					
29502820	1	4	theme	alginate/graphene	180:196	arg1	beads					204:208	sodium alginate/graphene oxide beads	173:208	sodium alginate/graphene oxide beads	173:208	Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as the activating agent.					
29502820	7	5	theme	thermal	958:964	arg1	stability					966:974	a superior thermal stability	947:974	a superior thermal stability	947:974	Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase.					
29502820	1	6	theme	activating	293:302	arg1	agent					304:308	the activating agent	289:308	the activating agent	289:308	Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as the activating agent.					
29502820	1	6	theme	activating	293:302	arg1	N					235:235	N	235:235	N	235:235	Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as the activating agent.					
29502820	7	7	contain	possessed	937:945	arg2	stability					988:996	storage stability	980:996	storage stability	980:996	Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase.					
29502820	7	7	contain	possessed	937:945	arg2	stability					966:974	a superior thermal stability	947:974	a superior thermal stability	947:974	Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase.					
29502820	7	7	contain	possessed	937:945	arg1	pectinase					927:935	the immobilized pectinase	911:935	the immobilized pectinase	911:935	Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase.					
29502820	5	8	theme	optimal	684:690	arg1	4.5					717:719	4.5	717:719	4.5	717:719	The optimal pH of free pectinase was 4.5, while that of immobilized pectinase was shifted to 4.0.					
29502820	5	8	theme	optimal	684:690	arg1	pH					692:693	The optimal pH	680:693	The optimal pH of free pectinase	680:711	The optimal pH of free pectinase was 4.5, while that of immobilized pectinase was shifted to 4.0.					
29502820	1	9	theme	oxide	198:202	arg1	beads					204:208	sodium alginate/graphene oxide beads	173:208	sodium alginate/graphene oxide beads	173:208	Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as the activating agent.					
29502820	0	10	theme	oxide	119:123	arg1	beads					135:139	sodium alginate/graphene oxide composite beads	94:139	sodium alginate/graphene oxide composite beads	94:139	Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads.					
29502820	3	11	theme	Immobilization	436:449	arg1	conditions					451:460	Immobilization conditions	436:460	Immobilization conditions	436:460	Immobilization conditions were optimized by Box-Behnken design and the response surface method.					
29502820	4	12	theme	pectinase	564:572	arg1	activity					536:543	The activity	532:543	The activity of the immobilized pectinase prepared under optimal conditions	532:606	The activity of the immobilized pectinase prepared under optimal conditions reached 1236.86 ± 40.21 U/g, with an enzyme activity recovery of 83.5%.					
29502820	5	13	theme	immobilized	736:746	arg1	pectinase					748:756	immobilized pectinase	736:756	immobilized pectinase	736:756	The optimal pH of free pectinase was 4.5, while that of immobilized pectinase was shifted to 4.0.					
29502820	8	14	theme	immobilized	1065:1075	arg1	pectinase					1077:1085	the immobilized pectinase	1061:1085	the immobilized pectinase	1061:1085	Reusability studies indicated that the immobilized pectinase retained 73% of initial activity after six times cycles.					
29502820	6	15	theme	optimal	782:788	arg1	temperature					790:800	The optimal temperature	778:800	The optimal temperature of immobilized pectinase	778:825	The optimal temperature of immobilized pectinase was increased to 60 °C, which was 10 °C higher than that of free form.					
29502820	9	16	theme	good	1157:1160	arg1	properties					1162:1171	these good properties	1151:1171	these good properties	1151:1171	Due to these good properties, such immobilized pectinase may find application in food industry.					
29502820	2	17	theme	electron	406:413	arg1	microscopy					415:424	scanning electron microscopy	397:424	scanning electron microscopy analyses	397:433	The immobilized pectinase was characterized by Fourier transform infrared spectra and scanning electron microscopy analyses.					
29502820	5	18	theme	pectinase	703:711	arg1	4.5					717:719	4.5	717:719	4.5	717:719	The optimal pH of free pectinase was 4.5, while that of immobilized pectinase was shifted to 4.0.					
29502820	5	18	theme	pectinase	703:711	arg1	pH					692:693	The optimal pH	680:693	The optimal pH of free pectinase	680:711	The optimal pH of free pectinase was 4.5, while that of immobilized pectinase was shifted to 4.0.					
29502820	5	19	theme	free	698:701	arg1	pectinase					703:711	free pectinase	698:711	free pectinase	698:711	The optimal pH of free pectinase was 4.5, while that of immobilized pectinase was shifted to 4.0.					
29502820	3	20	theme	response	507:514	arg1	method					524:529	the response surface method	503:529	the response surface method	503:529	Immobilization conditions were optimized by Box-Behnken design and the response surface method.					
29502820	0	21	theme	composite	125:133	arg1	beads					135:139	sodium alginate/graphene oxide composite beads	94:139	sodium alginate/graphene oxide composite beads	94:139	Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads.					
29502820	4	22	theme	%	677:677	arg1	recovery					661:668	an enzyme activity recovery	642:668	an enzyme activity recovery of 83.5%	642:677	The activity of the immobilized pectinase prepared under optimal conditions reached 1236.86 ± 40.21 U/g, with an enzyme activity recovery of 83.5%.					
29502820	2	23	theme	immobilized	315:325	arg1	pectinase					327:335	The immobilized pectinase	311:335	The immobilized pectinase	311:335	The immobilized pectinase was characterized by Fourier transform infrared spectra and scanning electron microscopy analyses.					
29502820	8	24	theme	activity	1111:1118	arg1	activity					1111:1118	initial activity	1103:1118	initial activity	1103:1118	Reusability studies indicated that the immobilized pectinase retained 73% of initial activity after six times cycles.					
29502820	8	24	theme	activity	1111:1118	arg1	%					1098:1098	73%	1096:1098	73% of initial activity	1096:1118	Reusability studies indicated that the immobilized pectinase retained 73% of initial activity after six times cycles.					
29502820	7	25	theme	free	1010:1013	arg1	pectinase					1015:1023	free pectinase	1010:1023	free pectinase	1010:1023	Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase.					
29502820	4	26	theme	immobilized	552:562	arg1	pectinase					564:572	the immobilized pectinase	548:572	the immobilized pectinase prepared under optimal conditions	548:606	The activity of the immobilized pectinase prepared under optimal conditions reached 1236.86 ± 40.21 U/g, with an enzyme activity recovery of 83.5%.					
29502820	1	27	theme	amide	214:218	arg1	bonds					220:224	amide bonds	214:224	amide bonds	214:224	Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as the activating agent.					
29502820	4	28	theme	activity	652:659	arg1	recovery					661:668	an enzyme activity recovery	642:668	an enzyme activity recovery of 83.5%	642:677	The activity of the immobilized pectinase prepared under optimal conditions reached 1236.86 ± 40.21 U/g, with an enzyme activity recovery of 83.5%.					
29502820	8	29	theme	times	1130:1134	arg1	cycles					1136:1141	six times cycles	1126:1141	six times cycles	1126:1141	Reusability studies indicated that the immobilized pectinase retained 73% of initial activity after six times cycles.					
29502820	3	30	theme	surface	516:522	arg1	method					524:529	the response surface method	503:529	the response surface method	503:529	Immobilization conditions were optimized by Box-Behnken design and the response surface method.					
29502820	4	31	theme	enzyme	645:650	arg1	recovery					661:668	an enzyme activity recovery	642:668	an enzyme activity recovery of 83.5%	642:677	The activity of the immobilized pectinase prepared under optimal conditions reached 1236.86 ± 40.21 U/g, with an enzyme activity recovery of 83.5%.					
29502820	9	32	theme	food	1225:1228	arg1	industry					1230:1237	food industry	1225:1237	food industry	1225:1237	Due to these good properties, such immobilized pectinase may find application in food industry.					
29502820	2	33	theme	microscopy	415:424	arg1	analyses					426:433	scanning electron microscopy analyses	397:433	scanning electron microscopy analyses	397:433	The immobilized pectinase was characterized by Fourier transform infrared spectra and scanning electron microscopy analyses.					
29502820	9	34	theme	such	1174:1177	arg1	pectinase					1191:1199	such immobilized pectinase	1174:1199	such immobilized pectinase	1174:1199	Due to these good properties, such immobilized pectinase may find application in food industry.					
29502820	9	35	from	application	1210:1220	arg1	industry					1230:1237	food industry	1225:1237	food industry	1225:1237	Due to these good properties, such immobilized pectinase may find application in food industry.					
29502820	9	36	theme	immobilized	1179:1189	arg1	pectinase					1191:1199	such immobilized pectinase	1174:1199	such immobilized pectinase	1174:1199	Due to these good properties, such immobilized pectinase may find application in food industry.					
29502820	0	37	theme	catalytic	34:42	arg1	behavior					44:51	catalytic behavior	34:51	catalytic behavior	34:51	Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads.					
29502820	7	38	theme	immobilized	915:925	arg1	pectinase					927:935	the immobilized pectinase	911:935	the immobilized pectinase	911:935	Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase.					
29502820	0	39	theme	pectinase	56:64	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads.					
29502820	0	39	theme	pectinase	56:64	arg1	behavior					44:51	catalytic behavior	34:51	catalytic behavior	34:51	Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads.					
29502820	0	39	theme	pectinase	56:64	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads.					
29502820	2	40	dep	Fourier	358:364	arg1	transform					366:374	transform	366:374	transform infrared spectra and scanning electron microscopy analyses	366:433	The immobilized pectinase was characterized by Fourier transform infrared spectra and scanning electron microscopy analyses.					
29502820	2	41	theme	scanning	397:404	arg1	microscopy					415:424	scanning electron microscopy	397:424	scanning electron microscopy analyses	397:433	The immobilized pectinase was characterized by Fourier transform infrared spectra and scanning electron microscopy analyses.					
29502820	7	42	theme	storage	980:986	arg1	stability					988:996	storage stability	980:996	storage stability	980:996	Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase.					
29502820	1	43	theme	N'-dicyclohexylcarbodiimide	237:263	arg1	N					235:235	N	235:235	N	235:235	Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as the activating agent.					
29502820	1	43	theme	N'-dicyclohexylcarbodiimide	237:263	arg1	/N-hydroxysuccinimide					264:284	N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide	237:284	N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide	237:284	Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as the activating agent.					
29502820	6	44	theme	pectinase	817:825	arg1	temperature					790:800	The optimal temperature	778:800	The optimal temperature of immobilized pectinase	778:825	The optimal temperature of immobilized pectinase was increased to 60 °C, which was 10 °C higher than that of free form.					
29502820	2	45	dep	transform	366:374	arg1	infrared					376:383	infrared	376:383	transform infrared spectra and scanning electron microscopy analyses	366:433	The immobilized pectinase was characterized by Fourier transform infrared spectra and scanning electron microscopy analyses.					
29502820	6	46	theme	free	887:890	arg1	form					892:895	free form	887:895	free form	887:895	The optimal temperature of immobilized pectinase was increased to 60 °C, which was 10 °C higher than that of free form.					
29502820	6	47	theme	immobilized	805:815	arg1	pectinase					817:825	immobilized pectinase	805:825	immobilized pectinase	805:825	The optimal temperature of immobilized pectinase was increased to 60 °C, which was 10 °C higher than that of free form.					
29502820	3	48	theme	Box-Behnken	480:490	arg1	design					492:497	Box-Behnken design	480:497	Box-Behnken design	480:497	Immobilization conditions were optimized by Box-Behnken design and the response surface method.					
29502820	1	49	theme	sodium	173:178	arg1	beads					204:208	sodium alginate/graphene oxide beads	173:208	sodium alginate/graphene oxide beads	173:208	Pectinase was immobilized onto sodium alginate/graphene oxide beads via amide bonds by using N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as the activating agent.					
29502820	7	50	theme	superior	949:956	arg1	stability					966:974	a superior thermal stability	947:974	a superior thermal stability	947:974	Furthermore, the immobilized pectinase possessed a superior thermal stability and storage stability to those of free pectinase.					
29502820	0	51	theme	alginate/graphene	101:117	arg1	beads					135:139	sodium alginate/graphene oxide composite beads	94:139	sodium alginate/graphene oxide composite beads	94:139	Preparation, characterization and catalytic behavior of pectinase covalently immobilized onto sodium alginate/graphene oxide composite beads.					
31614751	4	0	from	acids	836:840	arg1	contents					863:870	the cecal contents	853:870	the cecal contents of the mice	853:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	7	1	theme	groEL	1317:1321	arg1	sequencing					1323:1332	groEL sequencing	1317:1332	groEL sequencing	1317:1332	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	3	2	theme	V3-V4	639:643	arg1	region					645:650	the V3-V4 region	635:650	the V3-V4 region of 16S rRNA and groEL gene, respectively	635:691	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	3	3	dep	diet	500:503	arg1	%					475:475	%	475:475	%	475:475	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	6	4	theme	luminal	1286:1292	arg1	contents					1294:1301	the luminal contents	1282:1301	the luminal contents	1282:1301	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	4	5	theme	cecal	857:861	arg1	contents					863:870	the cecal contents	853:870	the cecal contents of the mice	853:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	0	6	theme	Bifidobacterium	156:170	arg1	Composition					172:182	the Bifidobacterium Composition	152:182	Especially the Bifidobacterium Composition	141:182	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	0	6	theme	Bifidobacterium	156:170	arg1	Effects					30:36	the Effects	26:36	the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice	26:138	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	8	7	dep	intestine	1565:1573	arg1	either					1576:1581	either	1576:1581	either	1576:1581	These results indicated that FOS can selectively promote B. pseudolongum proliferation in the intestine, either in the lumen or the mucosa from the cecum to the distal colon.					
31614751	4	8	theme	total	812:816	arg1	SCFAs					843:847	SCFAs	843:847	SCFAs	843:847	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	8	theme	total	812:816	arg1	acids					836:840	total short-chain fatty acids	812:840	total short-chain fatty acids (SCFAs) in the cecal contents of the mice	812:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	5	9	from	increase	939:946	arg1	abundance					964:972	the relative abundance	951:972	the relative abundance of Actinobacteria	951:990	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	5	9	from	increase	939:946	arg1	contents					999:1006	the contents	995:1006	the contents	995:1006	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	9	10	theme	competitive	1689:1699	arg1	advantage					1701:1709	the competitive advantage	1685:1709	the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS	1685:1819	Further studies are required to reveal the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS.					
31614751	7	11	theme	sole	1392:1395	arg1	species					1413:1419	the sole bifidobacterial species	1388:1419	the sole bifidobacterial species	1388:1419	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	7	11	theme	sole	1392:1395	arg1	pseudolongum					1364:1375	Bifidobacterium pseudolongum	1348:1375	Bifidobacterium pseudolongum	1348:1375	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	4	12	theme	acids	836:840	arg1	SCFAs					843:847	SCFAs	843:847	SCFAs	843:847	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	12	theme	acids	836:840	arg1	acids					836:840	total short-chain fatty acids	812:840	total short-chain fatty acids (SCFAs) in the cecal contents of the mice	812:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	12	theme	acids	836:840	arg1	amount					802:807	the amount	798:807	the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice	798:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	12	theme	acids	836:840	arg1	increases					741:749	significant increases	729:749	significant increases in the total and wall weights of the cecum	729:792	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	5	13	from	mucosa	1012:1017	arg1	abundance					964:972	the relative abundance	951:972	the relative abundance of Actinobacteria	951:990	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	5	13	from	mucosa	1012:1017	arg1	contents					999:1006	the contents	995:1006	the contents	995:1006	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	4	14	theme	mice	879:882	arg1	contents					863:870	the cecal contents	853:870	the cecal contents of the mice	853:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	6	15	theme	genus	1196:1200	arg1	level					1202:1206	the genus level	1192:1206	the genus level	1192:1206	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	5	16	theme	relative	955:962	arg1	abundance					964:972	the relative abundance	951:972	the relative abundance of Actinobacteria	951:990	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	1	17	from	growth	288:293	arg1	gut					321:323	the gut	317:323	the gut	317:323	Fructooligosaccharides (FOS) are considered prebiotics and have been proven to selectively promote the growth of Bifidobacterium in the gut.					
31614751	5	18	theme	FOS	1061:1063	arg1	group					1065:1069	the FOS group	1057:1069	the FOS group	1057:1069	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	7	19	from	species	1413:1419	arg1	contents					1436:1443	the luminal contents	1424:1443	the luminal contents (>98%)	1424:1450	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	7	19	from	species	1413:1419	arg1	%					1449:1449	>98%	1446:1449	>98%	1446:1449	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	7	19	from	species	1413:1419	arg1	mucosa					1456:1461	mucosa	1456:1461	mucosa (>89%)	1456:1468	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	7	19	from	species	1413:1419	arg1	%					1467:1467	>89%	1464:1467	>89%	1464:1467	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	0	20	theme	Luminal	92:98	arg1	Composition					77:87	the Composition	73:87	the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice	73:138	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	6	21	theme	significant	1099:1109	arg1	increase					1111:1118	a significant increase	1097:1118	a significant increase	1097:1118	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	8	22	theme	distal	1632:1637	arg1	colon					1639:1643	the distal colon	1628:1643	the distal colon	1628:1643	These results indicated that FOS can selectively promote B. pseudolongum proliferation in the intestine, either in the lumen or the mucosa from the cecum to the distal colon.					
31614751	0	23	theme	Metagenomic	0:10	arg1	Insights					12:19	Metagenomic Insights	0:19	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.	0:183	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	4	24	theme	fatty	830:834	arg1	SCFAs					843:847	SCFAs	843:847	SCFAs	843:847	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	24	theme	fatty	830:834	arg1	acids					836:840	total short-chain fatty acids	812:840	total short-chain fatty acids (SCFAs) in the cecal contents of the mice	812:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	2	25	theme	intake	373:378	arg1	effects					358:364	the effects	354:364	the effects of FOS intake on the composition of luminal and mucosal microbiota in mice	354:439	This study aimed to clarify the effects of FOS intake on the composition of luminal and mucosal microbiota in mice.					
31614751	0	26	from	Effects	30:36	arg1	Composition					77:87	the Composition	73:87	the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice	73:138	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	5	27	from	cecum	1028:1032	arg1	increase					939:946	a significant increase	925:946	a significant increase in the relative abundance of Actinobacteria in the contents	925:1006	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	5	27	from	cecum	1028:1032	arg1	mucosa					1012:1017	mucosa	1012:1017	mucosa from the cecum to the distal colon	1012:1052	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	8	28	dep	B.	1528:1529	arg1	pseudolongum					1531:1542	pseudolongum	1531:1542	pseudolongum	1531:1542	These results indicated that FOS can selectively promote B. pseudolongum proliferation in the intestine, either in the lumen or the mucosa from the cecum to the distal colon.					
31614751	8	29	theme	B.	1528:1529	arg1	proliferation					1544:1556	B. pseudolongum proliferation	1528:1556	B. pseudolongum proliferation	1528:1556	These results indicated that FOS can selectively promote B. pseudolongum proliferation in the intestine, either in the lumen or the mucosa from the cecum to the distal colon.					
31614751	2	30	theme	FOS	369:371	arg1	intake					373:378	FOS intake	369:378	FOS intake	369:378	This study aimed to clarify the effects of FOS intake on the composition of luminal and mucosal microbiota in mice.					
31614751	2	31	theme	microbiota	422:431	arg1	composition					387:397	the composition	383:397	the composition of luminal and mucosal microbiota in mice	383:439	This study aimed to clarify the effects of FOS intake on the composition of luminal and mucosal microbiota in mice.					
31614751	4	32	theme	total	758:762	arg1	weights					773:779	the total and wall weights	754:779	weights	773:779	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	2	33	from	effects	358:364	arg1	composition					387:397	the composition	383:397	the composition of luminal and mucosal microbiota in mice	383:439	This study aimed to clarify the effects of FOS intake on the composition of luminal and mucosal microbiota in mice.					
31614751	4	34	theme	wall	768:771	arg1	weights					773:779	the total and wall weights	754:779	weights	773:779	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	2	35	theme	mucosal	414:420	arg1	microbiota					422:431	luminal and mucosal microbiota	402:431	luminal and mucosal microbiota	402:431	This study aimed to clarify the effects of FOS intake on the composition of luminal and mucosal microbiota in mice.					
31614751	3	36	theme	groEL	668:672	arg1	gene					674:677	groEL gene	668:677	groEL gene	668:677	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	0	37	theme	Microbiota	112:121	arg1	Composition					77:87	the Composition	73:87	the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice	73:138	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	6	38	from	level	1202:1206	arg1	related					1232:1238	related	1232:1238	related	1232:1238	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	6	38	from	level	1202:1206	arg1	abundance					1149:1157	the relative abundance	1136:1157	the relative abundance	1136:1157	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	2	39	theme	luminal	402:408	arg1	microbiota					422:431	luminal and mucosal microbiota	402:431	luminal and mucosal microbiota	402:431	This study aimed to clarify the effects of FOS intake on the composition of luminal and mucosal microbiota in mice.					
31614751	3	40	theme	16S	655:657	arg1	rRNA					659:662	16S rRNA	655:662	16S rRNA	655:662	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	0	41	theme	Mucosal	104:110	arg1	Microbiota					112:121	Mucosal Microbiota	104:121	Mucosal Microbiota	104:121	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	3	42	theme	microbiota	564:573	arg1	composition					529:539	the composition	525:539	the composition of luminal and mucosal microbiota, especially the Bifidobacterium,	525:606	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	3	43	theme	-supplemented	486:498	arg1	w/w					482:484	w/w	482:484	w/w	482:484	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	3	43	theme	-supplemented	486:498	arg1	diet					500:503	a 0% or 25% FOS (w/w)-supplemented diet	465:503	a 0% or 25% FOS (w/w)-supplemented diet for four weeks	465:518	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	9	44	theme	FOS-metabolizing	1741:1756	arg1	bacteria					1758:1765	other FOS-metabolizing bacteria	1735:1765	other FOS-metabolizing bacteria	1735:1765	Further studies are required to reveal the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS.					
31614751	5	45	theme	distal	1041:1046	arg1	colon					1048:1052	the distal colon	1037:1052	the distal colon	1037:1052	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	3	46	theme	rRNA	659:662	arg1	region					645:650	the V3-V4 region	635:650	the V3-V4 region of 16S rRNA and groEL gene, respectively	635:691	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	0	47	theme	C57BL/6J	126:133	arg1	Mice					135:138	C57BL/6J Mice	126:138	C57BL/6J Mice	126:138	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	5	48	theme	significant	927:937	arg1	increase					939:946	a significant increase	925:946	a significant increase in the relative abundance of Actinobacteria in the contents	925:1006	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	6	49	located	observed	1124:1131	arg1	related					1232:1238	related	1232:1238	related	1232:1238	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	6	49	located	observed	1124:1131	arg2	increase					1111:1118	a significant increase	1097:1118	a significant increase	1097:1118	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	6	49	located	observed	1124:1131	arg1	abundance					1149:1157	the relative abundance	1136:1157	the relative abundance	1136:1157	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	1	50	theme	Bifidobacterium	298:312	arg1	growth					288:293	the growth	284:293	the growth of Bifidobacterium in the gut	284:323	Fructooligosaccharides (FOS) are considered prebiotics and have been proven to selectively promote the growth of Bifidobacterium in the gut.					
31614751	4	51	theme	short-chain	818:828	arg1	SCFAs					843:847	SCFAs	843:847	SCFAs	843:847	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	51	theme	short-chain	818:828	arg1	acids					836:840	total short-chain fatty acids	812:840	total short-chain fatty acids (SCFAs) in the cecal contents of the mice	812:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	6	52	from	levels	1272:1277	arg1	contents					1294:1301	the luminal contents	1282:1301	the luminal contents	1282:1301	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	6	53	theme	butyric	1259:1265	arg1	acid					1267:1270	butyric acid	1259:1270	butyric acid levels in the luminal contents	1259:1301	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	4	54	from	increases	741:749	arg1	weights					773:779	the total and wall weights	754:779	weights	773:779	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	54	from	increases	741:749	arg1	contents					863:870	the cecal contents	853:870	the cecal contents of the mice	853:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	6	55	theme	relative	1140:1147	arg1	related					1232:1238	related	1232:1238	related	1232:1238	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	6	55	theme	relative	1140:1147	arg1	abundance					1149:1157	the relative abundance	1136:1157	the relative abundance	1136:1157	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	6	56	from	abundance	1149:1157	arg1	samples					1181:1187	all samples	1177:1187	all samples	1177:1187	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	6	57	theme	Coprococcus	1162:1172	arg1	related					1232:1238	related	1232:1238	related	1232:1238	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	6	57	theme	Coprococcus	1162:1172	arg1	abundance					1149:1157	the relative abundance	1136:1157	the relative abundance	1136:1157	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	3	58	theme	mucosal	556:562	arg1	microbiota					564:573	luminal and mucosal microbiota	544:573	luminal and mucosal microbiota	544:573	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	3	58	theme	mucosal	556:562	arg1	Bifidobacterium					591:605	the Bifidobacterium	587:605	especially the Bifidobacterium	576:605	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	5	59	theme	phylum	892:897	arg1	level					899:903	the phylum level	888:903	the phylum level	888:903	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	6	60	theme	acid	1267:1270	arg1	levels					1272:1277	butyric acid levels	1259:1277	butyric acid levels in the luminal contents	1259:1301	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	3	61	dep	%	475:475	arg1	%					468:468	%	468:468	%	468:468	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	3	61	dep	%	475:475	arg1	25					473:474	25	473:474	25	473:474	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	0	62	theme	Fructooligosaccharides	41:62	arg1	Composition					172:182	the Bifidobacterium Composition	152:182	Especially the Bifidobacterium Composition	141:182	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	0	62	theme	Fructooligosaccharides	41:62	arg1	Effects					30:36	the Effects	26:36	the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice	26:138	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	3	63	theme	gene	674:677	arg1	region					645:650	the V3-V4 region	635:650	the V3-V4 region of 16S rRNA and groEL gene, respectively	635:691	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	4	64	theme	cecum	788:792	arg1	weights					773:779	the total and wall weights	754:779	weights	773:779	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	65	from	amount	802:807	arg1	weights					773:779	the total and wall weights	754:779	weights	773:779	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	65	from	amount	802:807	arg1	contents					863:870	the cecal contents	853:870	the cecal contents of the mice	853:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	66	theme	significant	729:739	arg1	increases					741:749	significant increases	729:749	significant increases in the total and wall weights of the cecum	729:792	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	9	67	theme	response	1775:1782	arg1	mechanisms					1784:1793	the response mechanisms	1771:1793	the response mechanisms of B. pseudolongum to FOS	1771:1819	Further studies are required to reveal the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS.					
31614751	8	68	from	cecum	1619:1623	arg1	lumen					1590:1594	lumen	1590:1594	lumen	1590:1594	These results indicated that FOS can selectively promote B. pseudolongum proliferation in the intestine, either in the lumen or the mucosa from the cecum to the distal colon.					
31614751	8	68	from	cecum	1619:1623	arg1	mucosa					1603:1608	the mucosa	1599:1608	the mucosa	1599:1608	These results indicated that FOS can selectively promote B. pseudolongum proliferation in the intestine, either in the lumen or the mucosa from the cecum to the distal colon.					
31614751	9	69	theme	Further	1646:1652	arg1	studies					1654:1660	Further studies	1646:1660	Further studies	1646:1660	Further studies are required to reveal the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS.					
31614751	7	70	theme	Bifidobacterium	1348:1362	arg1	species					1413:1419	the sole bifidobacterial species	1388:1419	the sole bifidobacterial species	1388:1419	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	7	70	theme	Bifidobacterium	1348:1362	arg1	pseudolongum					1364:1375	Bifidobacterium pseudolongum	1348:1375	Bifidobacterium pseudolongum	1348:1375	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	4	71	theme	FOS	700:702	arg1	intervention					704:715	FOS intervention	700:715	FOS intervention	700:715	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	72	from	contents	863:870	arg1	SCFAs					843:847	SCFAs	843:847	SCFAs	843:847	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	72	from	contents	863:870	arg1	acids					836:840	total short-chain fatty acids	812:840	total short-chain fatty acids (SCFAs) in the cecal contents of the mice	812:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	72	from	contents	863:870	arg1	amount					802:807	the amount	798:807	the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice	798:882	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	4	72	from	contents	863:870	arg1	increases					741:749	significant increases	729:749	significant increases in the total and wall weights of the cecum	729:792	After FOS intervention, there were significant increases in the total and wall weights of the cecum and the amount of total short-chain fatty acids (SCFAs) in the cecal contents of the mice.					
31614751	3	73	theme	FOS	477:479	arg1	w/w					482:484	w/w	482:484	w/w	482:484	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	3	73	theme	FOS	477:479	arg1	diet					500:503	a 0% or 25% FOS (w/w)-supplemented diet	465:503	a 0% or 25% FOS (w/w)-supplemented diet for four weeks	465:518	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	6	74	from	increase	1247:1254	arg1	levels					1272:1277	butyric acid levels	1259:1277	butyric acid levels in the luminal contents	1259:1301	Besides Bifidobacterium, a significant increase was observed in the relative abundance of Coprococcus in all samples at the genus level, which may be partially related to the increase in butyric acid levels in the luminal contents.					
31614751	3	75	theme	luminal	544:550	arg1	microbiota					564:573	luminal and mucosal microbiota	544:573	luminal and mucosal microbiota	544:573	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	3	75	theme	luminal	544:550	arg1	Bifidobacterium					591:605	the Bifidobacterium	587:605	especially the Bifidobacterium	576:605	Briefly, mice were fed a 0% or 25% FOS (w/w)-supplemented diet for four weeks, and the composition of luminal and mucosal microbiota, especially the Bifidobacterium, was analyzed by sequencing the V3-V4 region of 16S rRNA and groEL gene, respectively.					
31614751	2	76	from	composition	387:397	arg1	mice					436:439	mice	436:439	mice	436:439	This study aimed to clarify the effects of FOS intake on the composition of luminal and mucosal microbiota in mice.					
31614751	9	77	theme	other	1735:1739	arg1	bacteria					1758:1765	other FOS-metabolizing bacteria	1735:1765	other FOS-metabolizing bacteria	1735:1765	Further studies are required to reveal the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS.					
31614751	0	78	from	Composition	77:87	arg1	Mice					135:138	C57BL/6J Mice	126:138	C57BL/6J Mice	126:138	Metagenomic Insights into the Effects of Fructooligosaccharides (FOS) on the Composition of Luminal and Mucosal Microbiota in C57BL/6J Mice, Especially the Bifidobacterium Composition.					
31614751	7	79	theme	bifidobacterial	1397:1411	arg1	species					1413:1419	the sole bifidobacterial species	1388:1419	the sole bifidobacterial species	1388:1419	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	7	79	theme	bifidobacterial	1397:1411	arg1	pseudolongum					1364:1375	Bifidobacterium pseudolongum	1348:1375	Bifidobacterium pseudolongum	1348:1375	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	8	80	dep	lumen	1590:1594	arg1	the					1586:1588	the	1586:1588	the	1586:1588	These results indicated that FOS can selectively promote B. pseudolongum proliferation in the intestine, either in the lumen or the mucosa from the cecum to the distal colon.					
31614751	9	81	theme	pseudolongum	1717:1728	arg1	advantage					1701:1709	the competitive advantage	1685:1709	the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS	1685:1819	Further studies are required to reveal the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS.					
31614751	5	82	theme	Actinobacteria	977:990	arg1	abundance					964:972	the relative abundance	951:972	the relative abundance of Actinobacteria	951:990	At the phylum level, the results showed a significant increase in the relative abundance of Actinobacteria in the contents and mucosa from the cecum to the distal colon in the FOS group.					
31614751	9	83	theme	pseudolongum	1801:1812	arg1	mechanisms					1784:1793	the response mechanisms	1771:1793	the response mechanisms of B. pseudolongum to FOS	1771:1819	Further studies are required to reveal the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS.					
31614751	9	83	theme	pseudolongum	1801:1812	arg1	bacteria					1758:1765	other FOS-metabolizing bacteria	1735:1765	other FOS-metabolizing bacteria	1735:1765	Further studies are required to reveal the competitive advantage of B. pseudolongum over other FOS-metabolizing bacteria and the response mechanisms of B. pseudolongum to FOS.					
31614751	7	84	theme	luminal	1428:1434	arg1	contents					1436:1443	the luminal contents	1424:1443	the luminal contents (>98%)	1424:1450	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31614751	7	84	theme	luminal	1428:1434	arg1	%					1449:1449	>98%	1446:1449	>98%	1446:1449	Furthermore, groEL sequencing revealed that Bifidobacterium pseudolongum was almost the sole bifidobacterial species in the luminal contents (>98%) and mucosa (>89%).					
31893551	0	0	theme	compounds	71:79	arg1	composition					46:56	the composition	42:56	the composition of bioactive compounds and antioxidant activities in buckwheat	42:119	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts.					
31893551	2	1	theme	%	425:425	arg1	sucrose					427:433	3% sucrose	424:433	3% sucrose	424:433	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	1	theme	%	425:425	arg1	solutions					366:374	four different solutions	351:374	four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2	351:451	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	1	2	theme	nutritional	213:223	arg1	quality					225:231	nutritional quality	213:231	nutritional quality	213:231	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	2	3	theme	3	424:424	arg1	%					425:425	%	425:425	%	425:425	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	0	4	theme	antioxidant	85:95	arg1	activities					97:106	antioxidant activities	85:106	antioxidant activities	85:106	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts.					
31893551	0	5	from	Effect	0:5	arg1	composition					46:56	the composition	42:56	the composition of bioactive compounds and antioxidant activities in buckwheat	42:119	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts.					
31893551	4	6	theme	γ-aminobutyric	725:738	arg1	acid					740:743	γ-aminobutyric acid	725:743	γ-aminobutyric acid	725:743	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	5	7	theme	cytoprotective	924:937	arg1	effects					939:945	cytoprotective effects	924:945	cytoprotective effects	924:945	Elicitation with both sucrose and CaCl2 not only significantly enhanced the antioxidant activities but also exerted cytoprotective effects against oxidative damage in HepG2 cells and fibroblasts.					
31893551	6	8	theme	potential	1142:1150	arg1	benefits					1159:1166	potential health benefits	1142:1166	potential health benefits	1142:1166	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	4	9	theme	sprout	793:798	arg1	growth					800:805	sprout growth	793:805	sprout growth	793:805	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	4	10	theme	buckwheat	615:623	arg1	sprouts					625:631	buckwheat sprouts	615:631	buckwheat sprouts	615:631	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	0	11	theme	activities	97:106	arg1	composition					46:56	the composition	42:56	the composition of bioactive compounds and antioxidant activities in buckwheat	42:119	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts.					
31893551	5	12	theme	oxidative	955:963	arg1	damage					965:970	oxidative damage	955:970	oxidative damage in HepG2 cells and fibroblasts	955:1001	Elicitation with both sucrose and CaCl2 not only significantly enhanced the antioxidant activities but also exerted cytoprotective effects against oxidative damage in HepG2 cells and fibroblasts.					
31893551	5	13	from	damage	965:970	arg1	fibroblasts					991:1001	fibroblasts	991:1001	fibroblasts	991:1001	Elicitation with both sucrose and CaCl2 not only significantly enhanced the antioxidant activities but also exerted cytoprotective effects against oxidative damage in HepG2 cells and fibroblasts.					
31893551	5	13	from	damage	965:970	arg1	cells					981:985	HepG2 cells	975:985	HepG2 cells	975:985	Elicitation with both sucrose and CaCl2 not only significantly enhanced the antioxidant activities but also exerted cytoprotective effects against oxidative damage in HepG2 cells and fibroblasts.					
31893551	2	14	theme	different	356:364	arg1	sucrose					397:403	3% sucrose	394:403	3% sucrose	394:403	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	14	theme	different	356:364	arg1	CaCl2					413:417	7.5 mM CaCl2	406:417	7.5 mM CaCl2	406:417	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	14	theme	different	356:364	arg1	sucrose					427:433	3% sucrose	424:433	3% sucrose	424:433	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	14	theme	different	356:364	arg1	solutions					366:374	four different solutions	351:374	four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2	351:451	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	14	theme	different	356:364	arg1	water					387:391	distilled water	377:391	distilled water	377:391	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	0	15	theme	bioactive	61:69	arg1	compounds					71:79	bioactive compounds	61:79	bioactive compounds	61:79	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts.					
31893551	3	16	theme	sucrose-elicitation	505:523	arg1	reduction					540:548	sucrose-elicitation induced growth reduction	505:548	sucrose-elicitation induced growth reduction	505:548	Our results showed that CaCl2 effectively improved sucrose-elicitation induced growth reduction in buckwheat sprouts.					
31893551	1	17	theme	antioxidant	238:248	arg1	capacity					250:257	antioxidant capacity	238:257	antioxidant capacity	238:257	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	2	18	theme	Buckwheat	282:290	arg1	seeds					292:296	Buckwheat seeds	282:296	Buckwheat seeds	282:296	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	6	19	with	elicitation	1047:1057	arg1	sucrose					1067:1073	3% sucrose	1064:1073	3% sucrose	1064:1073	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	6	19	with	elicitation	1047:1057	arg1	CaCl2					1086:1090	7.5 mM CaCl2	1079:1090	7.5 mM CaCl2	1079:1090	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	0	20	theme	chloride	18:25	arg1	Effect					0:5	Effect	0:5	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat	0:119	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts.					
31893551	5	21	theme	HepG2	975:979	arg1	cells					981:985	HepG2 cells	975:985	HepG2 cells	975:985	Elicitation with both sucrose and CaCl2 not only significantly enhanced the antioxidant activities but also exerted cytoprotective effects against oxidative damage in HepG2 cells and fibroblasts.					
31893551	1	22	theme	sucrose	172:178	arg1	effect					162:167	the effect	158:167	the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat	158:279	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	2	23	theme	3	394:394	arg1	%					395:395	%	395:395	%	395:395	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	6	24	theme	buckwheat	1171:1179	arg1	sprouts					1181:1187	buckwheat sprouts	1171:1187	buckwheat sprouts	1171:1187	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	6	25	theme	nutritional	1120:1130	arg1	value					1132:1136	the nutritional value	1116:1136	the nutritional value	1116:1136	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	0	26	theme	sucrose	31:37	arg1	Effect					0:5	Effect	0:5	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat	0:119	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts.					
31893551	1	27	theme	sprouted	262:269	arg1	buckwheat					271:279	sprouted buckwheat	262:279	sprouted buckwheat	262:279	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	2	28	theme	distilled	377:385	arg1	solutions					366:374	four different solutions	351:374	four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2	351:451	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	28	theme	distilled	377:385	arg1	water					387:391	distilled water	377:391	distilled water	377:391	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	1	29	theme	CaCl2	184:188	arg1	effect					162:167	the effect	158:167	the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat	158:279	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	6	30	theme	%	1065:1065	arg1	sucrose					1067:1073	3% sucrose	1064:1073	3% sucrose	1064:1073	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	1	31	theme	buckwheat	271:279	arg1	profile					204:210	the growth profile	193:210	the growth profile	193:210	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	1	31	theme	buckwheat	271:279	arg1	quality					225:231	nutritional quality	213:231	nutritional quality	213:231	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	1	31	theme	buckwheat	271:279	arg1	capacity					250:257	antioxidant capacity	238:257	antioxidant capacity	238:257	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	2	32	theme	%	395:395	arg1	sucrose					397:403	3% sucrose	394:403	3% sucrose	394:403	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	32	theme	%	395:395	arg1	solutions					366:374	four different solutions	351:374	four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2	351:451	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	33	theme	7.5 mM	440:445	arg1	CaCl2					447:451	7.5 mM CaCl2	440:451	7.5 mM CaCl2	440:451	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	0	34	from	composition	46:56	arg1	buckwheat					111:119	buckwheat	111:119	buckwheat	111:119	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts.					
31893551	3	35	theme	growth	533:538	arg1	reduction					540:548	sucrose-elicitation induced growth reduction	505:548	sucrose-elicitation induced growth reduction	505:548	Our results showed that CaCl2 effectively improved sucrose-elicitation induced growth reduction in buckwheat sprouts.					
31893551	4	36	theme	compounds	681:689	arg1	accumulation					655:666	the accumulation	651:666	the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E,	651:762	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	2	37	theme	7.5 mM	406:411	arg1	CaCl2					413:417	7.5 mM CaCl2	406:417	7.5 mM CaCl2	406:417	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	37	theme	7.5 mM	406:411	arg1	solutions					366:374	four different solutions	351:374	four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2	351:451	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	4	38	theme	bioactive	671:679	arg1	compounds					681:689	bioactive compounds	671:689	bioactive compounds	671:689	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	4	38	theme	bioactive	671:679	arg1	acid					740:743	γ-aminobutyric acid	725:743	γ-aminobutyric acid	725:743	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	4	38	theme	bioactive	671:679	arg1	E					761:761	E	761:761	E	761:761	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	4	38	theme	bioactive	671:679	arg1	polyphenols					700:710	polyphenols	700:710	polyphenols	700:710	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	4	38	theme	bioactive	671:679	arg1	flavonoids					713:722	flavonoids	713:722	flavonoids	713:722	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	4	38	theme	bioactive	671:679	arg1	C					754:754	vitamin C	746:754	vitamin C	746:754	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	6	39	theme	sprouts	1181:1187	arg1	benefits					1159:1166	potential health benefits	1142:1166	potential health benefits	1142:1166	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	6	39	theme	sprouts	1181:1187	arg1	value					1132:1136	the nutritional value	1116:1136	the nutritional value	1116:1136	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	5	40	with	Elicitation	808:818	arg1	CaCl2					842:846	CaCl2	842:846	CaCl2	842:846	Elicitation with both sucrose and CaCl2 not only significantly enhanced the antioxidant activities but also exerted cytoprotective effects against oxidative damage in HepG2 cells and fibroblasts.					
31893551	5	40	with	Elicitation	808:818	arg1	sucrose					830:836	sucrose	830:836	sucrose	830:836	Elicitation with both sucrose and CaCl2 not only significantly enhanced the antioxidant activities but also exerted cytoprotective effects against oxidative damage in HepG2 cells and fibroblasts.					
31893551	3	41	theme	buckwheat	553:561	arg1	sprouts					563:569	buckwheat sprouts	553:569	buckwheat sprouts	553:569	Our results showed that CaCl2 effectively improved sucrose-elicitation induced growth reduction in buckwheat sprouts.					
31893551	6	42	theme	health	1152:1157	arg1	benefits					1159:1166	potential health benefits	1142:1166	potential health benefits	1142:1166	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	4	43	theme	vitamin	746:752	arg1	C					754:754	vitamin C	746:754	vitamin C	746:754	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	1	44	from	effect	162:167	arg1	profile					204:210	the growth profile	193:210	the growth profile	193:210	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	1	44	from	effect	162:167	arg1	quality					225:231	nutritional quality	213:231	nutritional quality	213:231	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	1	44	from	effect	162:167	arg1	capacity					250:257	antioxidant capacity	238:257	antioxidant capacity	238:257	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	5	45	theme	antioxidant	884:894	arg1	activities					896:905	the antioxidant activities	880:905	the antioxidant activities	880:905	Elicitation with both sucrose and CaCl2 not only significantly enhanced the antioxidant activities but also exerted cytoprotective effects against oxidative damage in HepG2 cells and fibroblasts.					
31893551	1	46	theme	growth	197:202	arg1	profile					204:210	the growth profile	193:210	the growth profile	193:210	In this study, we evaluated the effect of sucrose and CaCl2 on the growth profile, nutritional quality, and antioxidant capacity of sprouted buckwheat.					
31893551	6	47	theme	7.5 mM	1079:1084	arg1	CaCl2					1086:1090	7.5 mM CaCl2	1079:1090	7.5 mM CaCl2	1079:1090	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	2	48	dep	solutions	366:374	arg1	CaCl2					447:451	7.5 mM CaCl2	440:451	7.5 mM CaCl2	440:451	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	48	dep	solutions	366:374	arg1	solutions					366:374	four different solutions	351:374	four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2	351:451	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	48	dep	solutions	366:374	arg1	sucrose					397:403	3% sucrose	394:403	3% sucrose	394:403	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	48	dep	solutions	366:374	arg1	sucrose					427:433	3% sucrose	424:433	3% sucrose	424:433	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	48	dep	solutions	366:374	arg1	water					387:391	distilled water	377:391	distilled water	377:391	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	2	48	dep	solutions	366:374	arg1	CaCl2					413:417	7.5 mM CaCl2	406:417	7.5 mM CaCl2	406:417	Buckwheat seeds were germinated at 25 °C for 8 days and sprayed with four different solutions: distilled water, 3% sucrose, 7.5 mM CaCl2, and 3% sucrose plus 7.5 mM CaCl2.					
31893551	6	49	theme	simultaneous	1034:1045	arg1	elicitation					1047:1057	simultaneous elicitation	1034:1057	simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2	1034:1090	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	4	50	with	Elicitation	572:582	arg1	CaCl2					606:610	CaCl2	606:610	CaCl2	606:610	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	4	50	with	Elicitation	572:582	arg1	sucrose					594:600	sucrose	594:600	sucrose	594:600	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	6	51	theme	3	1064:1064	arg1	%					1065:1065	%	1065:1065	%	1065:1065	These findings suggested that simultaneous elicitation with 3% sucrose and 7.5 mM CaCl2 can potentially improve the nutritional value and potential health benefits of buckwheat sprouts.					
31893551	4	52	from	Elicitation	572:582	arg1	sprouts					625:631	buckwheat sprouts	615:631	buckwheat sprouts	615:631	Elicitation with both sucrose and CaCl2 in buckwheat sprouts markedly enhanced the accumulation of bioactive compounds, such as polyphenols, flavonoids, γ-aminobutyric acid, vitamin C, and E, without negatively affecting sprout growth.					
31893551	0	53	theme	calcium	10:16	arg1	chloride					18:25	calcium chloride	10:25	calcium chloride	10:25	Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts.					
31893551	3	54	theme	induced	525:531	arg1	reduction					540:548	sucrose-elicitation induced growth reduction	505:548	sucrose-elicitation induced growth reduction	505:548	Our results showed that CaCl2 effectively improved sucrose-elicitation induced growth reduction in buckwheat sprouts.					
31479672	7	0	theme	%	1503:1503	arg1	activities					1479:1488	maximum enzyme activities	1464:1488	maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively	1464:1549	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	4	1	theme	sulfate	842:848	arg1	%					856:856	80%	854:856	80%	854:856	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	1	theme	sulfate	842:848	arg1	concentration					806:818	the concentration	802:818	the concentration of saturated ammonium sulfate	802:848	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	1	2	from	9782	243:246	arg1	precipitated					201:212	precipitated	201:212	precipitated	201:212	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	9	3	with	CLEA	1956:1959	arg1	pores					1970:1974	few pores	1966:1974	few pores	1966:1974	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	7	4	from	60 °C	1531:1535	arg1	activities					1479:1488	maximum enzyme activities	1464:1488	maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively	1464:1549	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	7	4	from	60 °C	1531:1535	arg1	%					1495:1495	95%	1493:1495	95%	1493:1495	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	7	4	from	60 °C	1531:1535	arg1	%					1503:1503	80%	1501:1503	80% at temperatures 55 °C and 60 °C	1501:1535	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	9	5	theme	CLEA	1956:1959	arg1	clusters					1944:1951	the amorphous clusters	1930:1951	the amorphous clusters of CLEA with few pores	1930:1974	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	5	6	theme	increased	1169:1177	arg1	activities					1179:1188	increased activities	1169:1188	increased activities	1169:1188	Enzyme molecules covalently cross-linked with chitin beads showed increased activities compared to free enzymes and enzymes cross-linked with glutaraldehyde.					
31479672	9	7	theme	few	1966:1968	arg1	pores					1970:1974	few pores	1966:1974	few pores	1966:1974	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	9	8	theme	chitin-immobilized	1832:1849	arg1	microspheres					1860:1871	chitin-immobilized CLEA-MET microspheres	1832:1871	chitin-immobilized CLEA-MET microspheres	1832:1871	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	4	9	theme	saturated	823:831	arg1	sulfate					842:848	saturated ammonium sulfate	823:848	saturated ammonium sulfate	823:848	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	7	10	theme	cross-linked	1418:1429	arg1	CLEA-METs					1431:1439	chitin cross-linked CLEA-METs	1411:1439	chitin cross-linked CLEA-METs	1411:1439	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	4	11	theme	different	679:687	arg1	runs					692:695	the different 20 runs	675:695	the different 20 runs performed	675:705	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	6	12	theme	CLEA-METs	1329:1337	arg1	CLEA-METs					1363:1371	CLEA-METs and chitin-cross-linked CLEA-METs	1329:1371	CLEA-METs and chitin-cross-linked CLEA-METs	1329:1371	FTIR spectra confirmed the secondary structural alterations between CLEA-METs and chitin-cross-linked CLEA-METs.					
31479672	0	13	theme	efficient	124:132	arg1	formulations					146:157	efficient therapeutic formulations	124:157	efficient therapeutic formulations	124:157	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	9	14	theme	electron	1718:1725	arg1	microscopy					1727:1736	scanning electron microscopy	1709:1736	scanning electron microscopy	1709:1736	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	10	15	theme	A549	2090:2093	arg1	cells					2095:2099	A549 cells	2090:2099	A549 cells	2090:2099	Cytotoxicity analysis demonstrated that chitin-immobilized CLEA-MET significantly inhibited the proliferation of A549 cells up to 96.66% compared to free enzyme (72%) and CLEA-METs (76%).					
31479672	6	16	theme	structural	1298:1307	arg1	alterations					1309:1319	the secondary structural alterations	1284:1319	the secondary structural alterations between CLEA-METs and chitin-cross-linked CLEA-METs	1284:1371	FTIR spectra confirmed the secondary structural alterations between CLEA-METs and chitin-cross-linked CLEA-METs.					
31479672	7	17	theme	Thermal	1374:1380	arg1	assays					1392:1397	Thermal stability assays	1374:1397	Thermal stability assays	1374:1397	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	4	18	theme	highest	712:718	arg1	activity					727:734	the highest enzyme activity	708:734	the highest enzyme activity	708:734	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	9	19	theme	electron	1752:1759	arg1	microscopy					1761:1770	transmission electron microscopy	1739:1770	transmission electron microscopy	1739:1770	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	4	20	theme	enzyme	1086:1091	arg1	activity					1093:1100	enzyme activity	1086:1100	enzyme activity	1086:1100	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	1	21	theme	insoluble	344:352	arg1	enzyme					354:359	a catalytically active insoluble enzyme	321:359	a catalytically active insoluble enzyme	321:359	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	3	22	theme	agent	632:636	arg1	concentration					601:613	concentration	601:613	concentration of cross-linking agent	601:636	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	3	22	theme	agent	632:636	arg1	duration					643:650	duration	643:650	duration of cross linking	643:667	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	3	22	theme	agent	632:636	arg1	role					580:583	the role	576:583	the role of precipitant	576:598	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	7	23	link	cross-linked	1418:1429	arg1	CLEA-METs					1431:1439	chitin cross-linked CLEA-METs	1411:1439	chitin cross-linked CLEA-METs	1411:1439	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	10	24	theme	free	2126:2129	arg1	enzyme					2131:2136	free enzyme	2126:2136	free enzyme (72%)	2126:2142	Cytotoxicity analysis demonstrated that chitin-immobilized CLEA-MET significantly inhibited the proliferation of A549 cells up to 96.66% compared to free enzyme (72%) and CLEA-METs (76%).					
31479672	10	24	theme	free	2126:2129	arg1	%					2141:2141	72%	2139:2141	72%	2139:2141	Cytotoxicity analysis demonstrated that chitin-immobilized CLEA-MET significantly inhibited the proliferation of A549 cells up to 96.66% compared to free enzyme (72%) and CLEA-METs (76%).					
31479672	1	25	from	study	168:172	arg1	precipitated					201:212	precipitated	201:212	precipitated	201:212	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	9	26	theme	screening	1790:1798	arg1	technique					1808:1816	high content screening imaging technique	1777:1816	high content screening imaging technique	1777:1816	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	4	27	theme	R2	919:920	arg1	period					902:907	the incubation period	887:907	the incubation period	887:907	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	27	theme	R2	919:920	arg1	values					922:927	12 h. R2 values	913:927	12 h. R2 values of 0.9754 (enzyme activity)	913:955	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	2	28	theme	precipitating	440:452	arg1	efficiency					460:469	the highest precipitating (80%) efficiency	428:469	the highest precipitating (80%) efficiency	428:469	Among the various precipitants tested, ammonium sulfate displayed the highest precipitating (80%) efficiency.					
31479672	2	29	theme	ammonium	401:408	arg1	sulfate					410:416	ammonium sulfate	401:416	ammonium sulfate	401:416	Among the various precipitants tested, ammonium sulfate displayed the highest precipitating (80%) efficiency.					
31479672	1	30	theme	cross-linking	276:288	arg1	glutaraldehyde					296:309	a cross-linking agent glutaraldehyde	274:309	a cross-linking agent glutaraldehyde	274:309	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	9	31	theme	CLEA-MET	1851:1858	arg1	microspheres					1860:1871	chitin-immobilized CLEA-MET microspheres	1832:1871	chitin-immobilized CLEA-MET microspheres	1832:1871	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	8	32	theme	Storage	1552:1558	arg1	assays					1570:1575	Storage stability assays	1552:1575	Storage stability assays	1552:1575	Storage stability assays showed that CLEA-METs retained 65% of their initial activity and chitin-immobilized CLEAs retained 88% of their activity.					
31479672	3	33	theme	double-response	474:488	arg1	software					511:518	software	511:518	software that provides 20 different runs	511:550	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	3	33	theme	double-response	474:488	arg1	concept					502:508	A double-response statistical concept	472:508	A double-response statistical concept	472:508	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	0	34	theme	chitin	15:20	arg1	aggregates					42:51	chitin cross-linked enzyme aggregates	15:51	chitin cross-linked enzyme aggregates	15:51	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	3	35	theme	linking	661:667	arg1	cross					655:659	cross linking	655:667	cross linking	655:667	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	0	36	theme	enzyme	35:40	arg1	aggregates					42:51	chitin cross-linked enzyme aggregates	15:51	chitin cross-linked enzyme aggregates	15:51	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	9	37	theme	good	1880:1883	arg1	monodispersity					1885:1898	good monodispersity	1880:1898	good monodispersity	1880:1898	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	4	38	located	observed	740:747	arg2	activity					727:734	the highest enzyme activity	708:734	the highest enzyme activity	708:734	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	38	located	observed	740:747	arg1	run					752:754	run 6	752:756	run 6 (88.17 U)	752:766	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	38	located	observed	740:747	arg1	U					765:765	88.17 U	759:765	88.17 U	759:765	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	9	39	theme	mesoporous	1904:1913	arg1	structure					1915:1923	mesoporous structure	1904:1923	mesoporous structure	1904:1923	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	1	40	theme	putida	231:236	arg1	9782					243:246	Pseudomonas putida MTCC 9782	219:246	Pseudomonas putida MTCC 9782	219:246	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	4	41	theme	enhanced	1016:1023	arg1	association					1025:1035	an enhanced association	1013:1035	an enhanced association between the experimental and predicted values of enzyme activity	1013:1100	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	42	theme	aggregate	773:781	arg1	size					783:786	the aggregate size	769:786	the aggregate size	769:786	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	3	43	theme	different	537:545	arg1	runs					547:550	20 different runs	534:550	20 different runs	534:550	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	7	44	from	temperatures	1508:1519	arg1	activities					1479:1488	maximum enzyme activities	1464:1488	maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively	1464:1549	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	7	44	from	temperatures	1508:1519	arg1	%					1495:1495	95%	1493:1495	95%	1493:1495	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	7	44	from	temperatures	1508:1519	arg1	%					1503:1503	80%	1501:1503	80% at temperatures 55 °C and 60 °C	1501:1535	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	9	45	theme	amorphous	1934:1942	arg1	clusters					1944:1951	the amorphous clusters	1930:1951	the amorphous clusters of CLEA with few pores	1930:1974	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	9	46	theme	high	1777:1780	arg1	technique					1808:1816	high content screening imaging technique	1777:1816	high content screening imaging technique	1777:1816	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	6	47	theme	FTIR	1261:1264	arg1	spectra					1266:1272	FTIR spectra	1261:1272	FTIR spectra	1261:1272	FTIR spectra confirmed the secondary structural alterations between CLEA-METs and chitin-cross-linked CLEA-METs.					
31479672	10	48	theme	Cytotoxicity	1977:1988	arg1	analysis					1990:1997	Cytotoxicity analysis	1977:1997	Cytotoxicity analysis	1977:1997	Cytotoxicity analysis demonstrated that chitin-immobilized CLEA-MET significantly inhibited the proliferation of A549 cells up to 96.66% compared to free enzyme (72%) and CLEA-METs (76%).					
31479672	2	49	theme	various	372:378	arg1	precipitants					380:391	the various precipitants	368:391	the various precipitants tested	368:398	Among the various precipitants tested, ammonium sulfate displayed the highest precipitating (80%) efficiency.					
31479672	5	50	theme	chitin	1149:1154	arg1	beads					1156:1160	chitin beads	1149:1160	chitin beads	1149:1160	Enzyme molecules covalently cross-linked with chitin beads showed increased activities compared to free enzymes and enzymes cross-linked with glutaraldehyde.					
31479672	7	51	theme	%	1495:1495	arg1	activities					1479:1488	maximum enzyme activities	1464:1488	maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively	1464:1549	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	4	52	theme	ammonium	833:840	arg1	sulfate					842:848	saturated ammonium sulfate	823:848	saturated ammonium sulfate	823:848	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	0	53	link	cross-linked	22:33	arg1	aggregates					42:51	chitin cross-linked enzyme aggregates	15:51	chitin cross-linked enzyme aggregates	15:51	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	8	54	theme	activity	1629:1636	arg1	%					1610:1610	65%	1608:1610	65% of their initial activity	1608:1636	Storage stability assays showed that CLEA-METs retained 65% of their initial activity and chitin-immobilized CLEAs retained 88% of their activity.					
31479672	8	54	theme	activity	1629:1636	arg1	activity					1629:1636	their initial activity	1615:1636	their initial activity	1615:1636	Storage stability assays showed that CLEA-METs retained 65% of their initial activity and chitin-immobilized CLEAs retained 88% of their activity.					
31479672	7	55	theme	maximum	1464:1470	arg1	activities					1479:1488	maximum enzyme activities	1464:1488	maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively	1464:1549	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	4	56	theme	predicted	1066:1074	arg1	values					1076:1081	the experimental and predicted values	1045:1081	the experimental and predicted values of enzyme activity	1045:1100	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	8	57	theme	chitin-immobilized	1642:1659	arg1	CLEAs					1661:1665	chitin-immobilized CLEAs	1642:1665	chitin-immobilized CLEAs	1642:1665	Storage stability assays showed that CLEA-METs retained 65% of their initial activity and chitin-immobilized CLEAs retained 88% of their activity.					
31479672	7	58	theme	enzyme	1472:1477	arg1	activities					1479:1488	maximum enzyme activities	1464:1488	maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively	1464:1549	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	9	59	theme	content	1782:1788	arg1	technique					1808:1816	high content screening imaging technique	1777:1816	high content screening imaging technique	1777:1816	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	7	60	from	activities	1479:1488	arg1	temperatures					1508:1519	temperatures 55 °C and 60 °C	1508:1535	temperatures	1508:1519	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	7	60	from	activities	1479:1488	arg1	60 °C					1531:1535	60 °C	1531:1535	60 °C	1531:1535	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	0	61	theme	therapeutic	134:144	arg1	formulations					146:157	efficient therapeutic formulations	124:157	efficient therapeutic formulations	124:157	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	9	62	theme	scanning	1709:1716	arg1	microscopy					1727:1736	scanning electron microscopy	1709:1736	scanning electron microscopy	1709:1736	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	7	63	theme	chitin	1411:1416	arg1	CLEA-METs					1431:1439	chitin cross-linked CLEA-METs	1411:1439	chitin cross-linked CLEA-METs	1411:1439	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	4	64	theme	experimental	1049:1060	arg1	values					1076:1081	the experimental and predicted values	1045:1081	the experimental and predicted values of enzyme activity	1045:1100	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	10	65	theme	chitin-immobilized	2017:2034	arg1	CLEA-MET					2036:2043	chitin-immobilized CLEA-MET	2017:2043	chitin-immobilized CLEA-MET	2017:2043	Cytotoxicity analysis demonstrated that chitin-immobilized CLEA-MET significantly inhibited the proliferation of A549 cells up to 96.66% compared to free enzyme (72%) and CLEA-METs (76%).					
31479672	1	66	dep	putida	231:236	arg1	Pseudomonas					219:229	Pseudomonas	219:229	Pseudomonas	219:229	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	1	67	theme	active	337:342	arg1	enzyme					354:359	a catalytically active insoluble enzyme	321:359	a catalytically active insoluble enzyme	321:359	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	6	68	theme	secondary	1288:1296	arg1	alterations					1309:1319	the secondary structural alterations	1284:1319	the secondary structural alterations between CLEA-METs and chitin-cross-linked CLEA-METs	1284:1371	FTIR spectra confirmed the secondary structural alterations between CLEA-METs and chitin-cross-linked CLEA-METs.					
31479672	3	69	theme	precipitant	588:598	arg1	concentration					601:613	concentration	601:613	concentration of cross-linking agent	601:636	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	3	69	theme	precipitant	588:598	arg1	duration					643:650	duration	643:650	duration of cross linking	643:667	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	3	69	theme	precipitant	588:598	arg1	role					580:583	the role	576:583	the role of precipitant	576:598	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	9	70	theme	transmission	1739:1750	arg1	microscopy					1761:1770	transmission electron microscopy	1739:1770	transmission electron microscopy	1739:1770	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	7	71	theme	stability	1382:1390	arg1	assays					1392:1397	Thermal stability assays	1374:1397	Thermal stability assays	1374:1397	Thermal stability assays showed that chitin cross-linked CLEA-METs and CLEA-METs retained maximum enzyme activities of 95% and 80% at temperatures 55 °C and 60 °C, respectively.					
31479672	1	72	from	precipitated	201:212	arg1	study					168:172	this study	163:172	this study	163:172	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	4	73	theme	glutaraldehyde	862:875	arg1	2 mM					877:880	glutaraldehyde 2 mM	862:880	glutaraldehyde 2 mM	862:880	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	74	theme	activity	1093:1100	arg1	values					1076:1081	the experimental and predicted values	1045:1081	the experimental and predicted values of enzyme activity	1045:1100	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	75	theme	enzyme	720:725	arg1	activity					727:734	the highest enzyme activity	708:734	the highest enzyme activity	708:734	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	0	76	theme	upgraded	75:82	arg1	activity					84:91	upgraded activity	75:91	upgraded activity	75:91	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	3	77	theme	cross-linking	618:630	arg1	agent					632:636	cross-linking agent	618:636	cross-linking agent	618:636	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	10	78	theme	cells	2095:2099	arg1	proliferation					2073:2085	the proliferation	2069:2085	the proliferation of A549 cells	2069:2099	Cytotoxicity analysis demonstrated that chitin-immobilized CLEA-MET significantly inhibited the proliferation of A549 cells up to 96.66% compared to free enzyme (72%) and CLEA-METs (76%).					
31479672	4	79	theme	0.9754	932:937	arg1	period					902:907	the incubation period	887:907	the incubation period	887:907	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	79	theme	0.9754	932:937	arg1	values					922:927	12 h. R2 values	913:927	12 h. R2 values of 0.9754 (enzyme activity)	913:955	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	9	80	theme	imaging	1800:1806	arg1	technique					1808:1816	high content screening imaging technique	1777:1816	high content screening imaging technique	1777:1816	Moreover, scanning electron microscopy, transmission electron microscopy, and high content screening imaging technique revealed that chitin-immobilized CLEA-MET microspheres showed good monodispersity and mesoporous structure with the amorphous clusters of CLEA with few pores.					
31479672	6	81	theme	chitin-cross-linked	1343:1361	arg1	CLEA-METs					1363:1371	CLEA-METs and chitin-cross-linked CLEA-METs	1329:1371	CLEA-METs and chitin-cross-linked CLEA-METs	1329:1371	FTIR spectra confirmed the secondary structural alterations between CLEA-METs and chitin-cross-linked CLEA-METs.					
31479672	4	82	theme	12 h.	913:917	arg1	period					902:907	the incubation period	887:907	the incubation period	887:907	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	82	theme	12 h.	913:917	arg1	values					922:927	12 h. R2 values	913:927	12 h. R2 values of 0.9754 (enzyme activity)	913:955	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	6	83	link	chitin-cross-linked	1343:1361	arg1	CLEA-METs					1363:1371	CLEA-METs and chitin-cross-linked CLEA-METs	1329:1371	CLEA-METs and chitin-cross-linked CLEA-METs	1329:1371	FTIR spectra confirmed the secondary structural alterations between CLEA-METs and chitin-cross-linked CLEA-METs.					
31479672	4	84	theme	incubation	891:900	arg1	period					902:907	the incubation period	887:907	the incubation period	887:907	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	84	theme	incubation	891:900	arg1	values					922:927	12 h. R2 values	913:927	12 h. R2 values of 0.9754 (enzyme activity)	913:955	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	8	85	theme	stability	1560:1568	arg1	assays					1570:1575	Storage stability assays	1552:1575	Storage stability assays	1552:1575	Storage stability assays showed that CLEA-METs retained 65% of their initial activity and chitin-immobilized CLEAs retained 88% of their activity.					
31479672	0	86	theme	cross-linked	22:33	arg1	aggregates					42:51	chitin cross-linked enzyme aggregates	15:51	chitin cross-linked enzyme aggregates	15:51	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	1	87	theme	agent	290:294	arg1	glutaraldehyde					296:309	a cross-linking agent glutaraldehyde	274:309	a cross-linking agent glutaraldehyde	274:309	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	4	88	theme	aggregate	969:977	arg1	0.9203					961:966	0.9203	961:966	0.9203	961:966	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	88	theme	aggregate	969:977	arg1	size					979:982	aggregate size	969:982	aggregate size	969:982	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	3	89	theme	cross	655:659	arg1	concentration					601:613	concentration	601:613	concentration of cross-linking agent	601:636	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	3	89	theme	cross	655:659	arg1	duration					643:650	duration	643:650	duration of cross linking	643:667	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	3	89	theme	cross	655:659	arg1	role					580:583	the role	576:583	the role of precipitant	576:598	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	3	90	theme	statistical	490:500	arg1	software					511:518	software	511:518	software that provides 20 different runs	511:550	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	3	90	theme	statistical	490:500	arg1	concept					502:508	A double-response statistical concept	472:508	A double-response statistical concept	472:508	A double-response statistical concept, software that provides 20 different runs, was employed to assess the role of precipitant, concentration of cross-linking agent, and duration of cross linking.					
31479672	0	91	theme	aggregates	42:51	arg1	Development					0:10	Development	0:10	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity	0:91	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	0	91	theme	aggregates	42:51	arg1	application					109:119	application	109:119	application	109:119	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	0	91	theme	aggregates	42:51	arg1	permanence					94:103	permanence	94:103	permanence	94:103	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	4	92	theme	enzyme	940:945	arg1	0.9754					932:937	0.9754	932:937	0.9754 (enzyme activity)	932:955	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	4	92	theme	enzyme	940:945	arg1	activity					947:954	enzyme activity	940:954	enzyme activity	940:954	From the different 20 runs performed, the highest enzyme activity was observed in run 6 (88.17 U): the aggregate size was 11.57 μm, the concentration of saturated ammonium sulfate was 80% and glutaraldehyde 2 mM, and the incubation period was 12 h. R2 values of 0.9754 (enzyme activity) and 0.9203 (aggregate size) were obtained, which showed an enhanced association between the experimental and predicted values of enzyme activity.					
31479672	0	93	theme	L-methioninase	56:69	arg1	Development					0:10	Development	0:10	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity	0:91	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	0	93	theme	L-methioninase	56:69	arg1	application					109:119	application	109:119	application	109:119	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	0	93	theme	L-methioninase	56:69	arg1	permanence					94:103	permanence	94:103	permanence	94:103	Development of chitin cross-linked enzyme aggregates of L-methioninase for upgraded activity, permanence and application as efficient therapeutic formulations.					
31479672	2	94	dep	precipitating	440:452	arg1	highest					432:438	highest	432:438	highest	432:438	Among the various precipitants tested, ammonium sulfate displayed the highest precipitating (80%) efficiency.					
31479672	2	94	dep	precipitating	440:452	arg1	%					457:457	80%	455:457	80%	455:457	Among the various precipitants tested, ammonium sulfate displayed the highest precipitating (80%) efficiency.					
31479672	5	95	theme	Enzyme	1103:1108	arg1	molecules					1110:1118	Enzyme molecules	1103:1118	Enzyme molecules covalently cross-linked with chitin beads	1103:1160	Enzyme molecules covalently cross-linked with chitin beads showed increased activities compared to free enzymes and enzymes cross-linked with glutaraldehyde.					
31479672	1	96	theme	MTCC	238:241	arg1	9782					243:246	Pseudomonas putida MTCC 9782	219:246	Pseudomonas putida MTCC 9782	219:246	In this study, L-methioninase (METs) was precipitated from Pseudomonas putida MTCC 9782 and was cross-linked with a cross-linking agent glutaraldehyde to obtain a catalytically active insoluble enzyme.					
31479672	8	97	theme	initial	1621:1627	arg1	activity					1629:1636	their initial activity	1615:1636	their initial activity	1615:1636	Storage stability assays showed that CLEA-METs retained 65% of their initial activity and chitin-immobilized CLEAs retained 88% of their activity.					
31479672	8	98	theme	activity	1689:1696	arg1	%					1678:1678	88%	1676:1678	88% of their activity	1676:1696	Storage stability assays showed that CLEA-METs retained 65% of their initial activity and chitin-immobilized CLEAs retained 88% of their activity.					
31479672	8	98	theme	activity	1689:1696	arg1	activity					1689:1696	their activity	1683:1696	their activity	1683:1696	Storage stability assays showed that CLEA-METs retained 65% of their initial activity and chitin-immobilized CLEAs retained 88% of their activity.					
31479672	5	99	theme	free	1202:1205	arg1	enzymes					1207:1213	free enzymes	1202:1213	free enzymes	1202:1213	Enzyme molecules covalently cross-linked with chitin beads showed increased activities compared to free enzymes and enzymes cross-linked with glutaraldehyde.					
31381927	4	0	theme	C1-oxidized	711:721	arg1	cello-oligosaccharides					723:744	C1-oxidized cello-oligosaccharides	711:744	C1-oxidized cello-oligosaccharides	711:744	This was confirmed subsequently by the enzymatic assays since MtLPMO9L only acts on cellulose and generates C1-oxidized cello-oligosaccharides.					
31381927	3	1	theme	extra	538:542	arg1	helix					544:548	an extra helix	535:548	an extra helix in the L3 loop	535:563	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	1	2	theme	polymers	285:292	arg1	application					312:322	the potential application	298:322	the potential application in biorefineries	298:339	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	1	2	theme	polymers	285:292	arg1	degradation					257:267	oxidative degradation	247:267	oxidative degradation of carbohydrate polymers	247:292	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	6	3	theme	used	956:959	arg1	MtLPMO9L					961:968	MtLPMO9L	961:968	MtLPMO9L	961:968	In contrast, the inhibitory rather than synergetic effect was observed when combining used MtLPMO9L and CBHI.					
31381927	8	4	theme	different	1120:1128	arg1	detail					1138:1143	a different synergy detail	1118:1143	a different synergy detail	1118:1143	This discovery suggests a different synergy detail between MtLPMO9L and two CBHs, which implies that the composition of cellulase cocktails may need reconsideration.					
31381927	6	5	theme	synergetic	910:919	arg1	effect					921:926	the inhibitory rather than synergetic effect	883:926	the inhibitory rather than synergetic effect	883:926	In contrast, the inhibitory rather than synergetic effect was observed when combining used MtLPMO9L and CBHI.					
31381927	0	6	theme	cellulose	114:122	arg1	hydrolysis					124:133	cellulose hydrolysis	114:133	cellulose hydrolysis	114:133	A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis.					
31381927	7	7	theme	concentration	1013:1025	arg1	ratio					1027:1031	concentration ratio	1013:1031	concentration ratio	1013:1031	Changing the incubation time and concentration ratio of MtLPMO9L and CBHI could attenuate the inhibitory effects.					
31381927	3	8	theme	L2	594:595	arg1	loop					597:600	the L2 loop	590:600	the L2 loop	590:600	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	3	9	contain	has	523:525	arg2	helix					544:548	an extra helix	535:548	an extra helix in the L3 loop	535:563	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	3	9	contain	has	523:525	arg1	LPMO					511:514	the C1-oxidizing LPMO	494:514	the C1-oxidizing LPMO	494:514	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	3	9	contain	has	523:525	arg2	region					580:585	extra loop region	569:585	extra loop region in the L2 loop	569:600	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	0	10	with	synergism	77:85	arg1	cellobiohydrolases					92:109	cellobiohydrolases	92:109	cellobiohydrolases in cellulose hydrolysis	92:133	A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis.					
31381927	3	11	theme	extra	569:573	arg1	region					580:585	extra loop region	569:585	extra loop region in the L2 loop	569:600	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	1	12	theme	potential	302:310	arg1	application					312:322	the potential application	298:322	the potential application in biorefineries	298:339	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	8	13	theme	cocktails	1224:1232	arg1	composition					1199:1209	the composition	1195:1209	the composition of cellulase cocktails	1195:1232	This discovery suggests a different synergy detail between MtLPMO9L and two CBHs, which implies that the composition of cellulase cocktails may need reconsideration.					
31381927	0	14	from	thermophila	57:67	arg1	monooxygenase					23:35	A lytic polysaccharide monooxygenase	0:35	A lytic polysaccharide monooxygenase from Myceliophthora thermophila	0:67	A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis.					
31381927	0	14	from	thermophila	57:67	arg1	synergism					77:85	its synergism	73:85	its synergism with cellobiohydrolases in cellulose hydrolysis	73:133	A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis.					
31381927	1	15	from	biorefineries	327:339	arg1	mechanism					234:242	their unique mechanism	221:242	their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries	221:339	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	3	16	theme	L3	557:558	arg1	loop					560:563	the L3 loop	553:563	the L3 loop	553:563	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	1	17	theme	vast	195:198	arg1	attention					200:208	vast attention	195:208	vast attention	195:208	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	8	18	theme	cellulase	1214:1222	arg1	cocktails					1224:1232	cellulase cocktails	1214:1232	cellulase cocktails	1214:1232	This discovery suggests a different synergy detail between MtLPMO9L and two CBHs, which implies that the composition of cellulase cocktails may need reconsideration.					
31381927	3	19	theme	enzyme	458:463	arg1	model					445:449	The structure model	431:449	The structure model of the enzyme	431:463	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	0	20	theme	lytic	2:6	arg1	monooxygenase					23:35	A lytic polysaccharide monooxygenase	0:35	A lytic polysaccharide monooxygenase from Myceliophthora thermophila	0:67	A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis.					
31381927	1	21	from	application	312:322	arg1	biorefineries					327:339	biorefineries	327:339	biorefineries	327:339	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	2	22	from	thermophila	400:410	arg1	LPMO					375:378	a novel LPMO	367:378	a novel LPMO	367:378	This study characterized a novel LPMO from Myceliophthora thermophila, denoted MtLPMO9L.					
31381927	1	23	theme	application	312:322	arg1	mechanism					234:242	their unique mechanism	221:242	their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries	221:339	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	7	24	theme	incubation	993:1002	arg1	time					1004:1007	incubation time	993:1007	incubation time	993:1007	Changing the incubation time and concentration ratio of MtLPMO9L and CBHI could attenuate the inhibitory effects.					
31381927	3	25	theme	loop	575:578	arg1	region					580:585	extra loop region	569:585	extra loop region in the L2 loop	569:600	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	4	26	theme	enzymatic	642:650	arg1	assays					652:657	the enzymatic assays	638:657	the enzymatic assays	638:657	This was confirmed subsequently by the enzymatic assays since MtLPMO9L only acts on cellulose and generates C1-oxidized cello-oligosaccharides.					
31381927	0	27	theme	polysaccharide	8:21	arg1	monooxygenase					23:35	A lytic polysaccharide monooxygenase	0:35	A lytic polysaccharide monooxygenase from Myceliophthora thermophila	0:67	A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis.					
31381927	8	28	theme	synergy	1130:1136	arg1	detail					1138:1143	a different synergy detail	1118:1143	a different synergy detail	1118:1143	This discovery suggests a different synergy detail between MtLPMO9L and two CBHs, which implies that the composition of cellulase cocktails may need reconsideration.					
31381927	0	29	from	cellobiohydrolases	92:109	arg1	hydrolysis					124:133	cellulose hydrolysis	114:133	cellulose hydrolysis	114:133	A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis.					
31381927	1	30	theme	unique	227:232	arg1	mechanism					234:242	their unique mechanism	221:242	their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries	221:339	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	0	31	theme	Myceliophthora	42:55	arg1	thermophila					57:67	Myceliophthora thermophila	42:67	Myceliophthora thermophila	42:67	A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis.					
31381927	6	32	located	observed	932:939	arg2	effect					921:926	the inhibitory rather than synergetic effect	883:926	the inhibitory rather than synergetic effect	883:926	In contrast, the inhibitory rather than synergetic effect was observed when combining used MtLPMO9L and CBHI.					
31381927	6	32	located	observed	932:939	arg1	contrast					873:880	contrast	873:880	contrast	873:880	In contrast, the inhibitory rather than synergetic effect was observed when combining used MtLPMO9L and CBHI.					
31381927	0	33	with	monooxygenase	23:35	arg1	cellobiohydrolases					92:109	cellobiohydrolases	92:109	cellobiohydrolases in cellulose hydrolysis	92:133	A lytic polysaccharide monooxygenase from Myceliophthora thermophila and its synergism with cellobiohydrolases in cellulose hydrolysis.					
31381927	3	34	theme	C1-oxidizing	498:509	arg1	LPMO					511:514	the C1-oxidizing LPMO	494:514	the C1-oxidizing LPMO	494:514	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	5	35	theme	cellobiohydrolase	842:858	arg1	efficiency					828:837	the efficiency	824:837	the efficiency of cellobiohydrolase (CBH) II	824:867	Moreover, synergetic experiments showed that MtLPMO9L significantly improves the efficiency of cellobiohydrolase (CBH) II.					
31381927	5	36	theme	synergetic	757:766	arg1	experiments					768:778	synergetic experiments	757:778	synergetic experiments	757:778	Moreover, synergetic experiments showed that MtLPMO9L significantly improves the efficiency of cellobiohydrolase (CBH) II.					
31381927	6	37	theme	inhibitory	887:896	arg1	effect					921:926	the inhibitory rather than synergetic effect	883:926	the inhibitory rather than synergetic effect	883:926	In contrast, the inhibitory rather than synergetic effect was observed when combining used MtLPMO9L and CBHI.					
31381927	2	38	theme	Myceliophthora	385:398	arg1	thermophila					400:410	Myceliophthora thermophila	385:410	Myceliophthora thermophila	385:410	This study characterized a novel LPMO from Myceliophthora thermophila, denoted MtLPMO9L.					
31381927	1	39	theme	Lytic	136:140	arg1	monooxygenases					157:170	Lytic polysaccharide monooxygenases	136:170	Lytic polysaccharide monooxygenases (LPMOs)	136:178	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	1	39	theme	Lytic	136:140	arg1	LPMOs					173:177	LPMOs	173:177	LPMOs	173:177	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	7	40	theme	CBHI	1049:1052	arg1	time					1004:1007	incubation time	993:1007	incubation time	993:1007	Changing the incubation time and concentration ratio of MtLPMO9L and CBHI could attenuate the inhibitory effects.					
31381927	7	40	theme	CBHI	1049:1052	arg1	ratio					1027:1031	concentration ratio	1013:1031	concentration ratio	1013:1031	Changing the incubation time and concentration ratio of MtLPMO9L and CBHI could attenuate the inhibitory effects.					
31381927	1	41	theme	polysaccharide	142:155	arg1	monooxygenases					157:170	Lytic polysaccharide monooxygenases	136:170	Lytic polysaccharide monooxygenases (LPMOs)	136:178	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	1	41	theme	polysaccharide	142:155	arg1	LPMOs					173:177	LPMOs	173:177	LPMOs	173:177	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	7	42	theme	inhibitory	1074:1083	arg1	effects					1085:1091	the inhibitory effects	1070:1091	the inhibitory effects	1070:1091	Changing the incubation time and concentration ratio of MtLPMO9L and CBHI could attenuate the inhibitory effects.					
31381927	3	43	from	helix	544:548	arg1	loop					560:563	the L3 loop	553:563	the L3 loop	553:563	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	3	44	from	region	580:585	arg1	loop					597:600	the L2 loop	590:600	the L2 loop	590:600	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	1	45	theme	oxidative	247:255	arg1	degradation					257:267	oxidative degradation	247:267	oxidative degradation of carbohydrate polymers	247:292	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	1	46	from	degradation	257:267	arg1	biorefineries					327:339	biorefineries	327:339	biorefineries	327:339	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	1	47	from	mechanism	234:242	arg1	biorefineries					327:339	biorefineries	327:339	biorefineries	327:339	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	7	48	theme	MtLPMO9L	1036:1043	arg1	time					1004:1007	incubation time	993:1007	incubation time	993:1007	Changing the incubation time and concentration ratio of MtLPMO9L and CBHI could attenuate the inhibitory effects.					
31381927	7	48	theme	MtLPMO9L	1036:1043	arg1	ratio					1027:1031	concentration ratio	1013:1031	concentration ratio	1013:1031	Changing the incubation time and concentration ratio of MtLPMO9L and CBHI could attenuate the inhibitory effects.					
31381927	2	49	theme	novel	369:373	arg1	LPMO					375:378	a novel LPMO	367:378	a novel LPMO	367:378	This study characterized a novel LPMO from Myceliophthora thermophila, denoted MtLPMO9L.					
31381927	1	50	theme	degradation	257:267	arg1	mechanism					234:242	their unique mechanism	221:242	their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries	221:339	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
31381927	7	51	dep	time	1004:1007	arg1	the					989:991	the	989:991	the	989:991	Changing the incubation time and concentration ratio of MtLPMO9L and CBHI could attenuate the inhibitory effects.					
31381927	3	52	theme	structure	435:443	arg1	model					445:449	The structure model	431:449	The structure model of the enzyme	431:463	The structure model of the enzyme indicated that it belongs to the C1-oxidizing LPMO, which has neither an extra helix in the L3 loop nor extra loop region in the L2 loop.					
31381927	1	53	theme	carbohydrate	272:283	arg1	polymers					285:292	carbohydrate polymers	272:292	carbohydrate polymers	272:292	Lytic polysaccharide monooxygenases (LPMOs) have attracted vast attention because of their unique mechanism of oxidative degradation of carbohydrate polymers and the potential application in biorefineries.					
30261267	0	0	theme	method	162:167	arg1	variation					116:124	variation	116:124	variation	116:124	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	0	0	theme	method	162:167	arg1	aspects					140:146	practical aspects	130:146	practical aspects of a pH shift method	130:167	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	0	0	theme	method	162:167	arg1	Application					103:113	Application	103:113	Application	103:113	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	1	1	theme	effective	230:238	arg1	formulation					174:184	The formulation	170:184	The formulation of amorphous solid dispersions (ASDs)	170:222	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	1	1	theme	effective	230:238	arg1	way					240:242	an effective way	227:242	an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs)	227:338	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	4	2	from	change	666:671	arg1	HCl					710:712	0.1 M HCl	704:712	0.1 M HCl	704:712	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	9	3	theme	improved	1456:1463	arg1	behavior					1477:1484	an improved dissolution behavior	1453:1484	an improved dissolution behavior	1453:1484	The combination of the acidic polymers with macrogolglycerol hydroxystearate showed an improved dissolution behavior at higher API contents.					
30261267	8	4	theme	acid-ethyl	1224:1233	arg1	copolymer					1244:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	4	theme	acid-ethyl	1224:1233	arg1	polymers					1203:1210	The acidic polymers	1192:1210	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate	1192:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	3	5	theme	spray	564:568	arg1	drying					570:575	spray drying	564:575	spray drying	564:575	ASDs with ketoconazole and different polymers were manufactured by spray drying and their characteristics as well as performance were analyzed.					
30261267	9	6	theme	higher	1489:1494	arg1	contents					1500:1507	higher API contents	1489:1507	higher API contents	1489:1507	The combination of the acidic polymers with macrogolglycerol hydroxystearate showed an improved dissolution behavior at higher API contents.					
30261267	10	7	theme	oral	1631:1634	arg1	bioavailability					1636:1650	the oral bioavailability	1627:1650	the oral bioavailability of poorly water-soluble drugs	1627:1680	The optimization of such formulations with different release-patterns plays an important role for the enhancement of the oral bioavailability of poorly water-soluble drugs.					
30261267	1	8	theme	amorphous	189:197	arg1	ASDs					218:221	ASDs	218:221	ASDs	218:221	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	1	8	theme	amorphous	189:197	arg1	dispersions					205:215	amorphous solid dispersions	189:215	amorphous solid dispersions (ASDs)	189:222	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	4	9	theme	enteric	898:904	arg1	formulation					906:916	an enteric formulation	895:916	an enteric formulation	895:916	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	4	10	theme	Dissolution	641:651	arg1	tests					653:657	Dissolution tests	641:657	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	641:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	0	11	theme	practical	130:138	arg1	aspects					140:146	practical aspects	130:146	practical aspects of a pH shift method	130:167	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	1	12	theme	dispersions	205:215	arg1	formulation					174:184	The formulation	170:184	The formulation of amorphous solid dispersions (ASDs)	170:222	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	1	12	theme	dispersions	205:215	arg1	way					240:242	an effective way	227:242	an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs)	227:338	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	4	13	theme	0.1 M	704:708	arg1	HCl					710:712	0.1 M HCl	704:712	0.1 M HCl	704:712	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	10	14	theme	water-soluble	1662:1674	arg1	drugs					1676:1680	poorly water-soluble drugs	1655:1680	poorly water-soluble drugs	1655:1680	The optimization of such formulations with different release-patterns plays an important role for the enhancement of the oral bioavailability of poorly water-soluble drugs.					
30261267	0	15	theme	pH	153:154	arg1	method					162:167	a pH shift method	151:167	a pH shift method	151:167	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	8	16	theme	macrogolglycerol	1306:1321	arg1	hydroxystearate					1323:1337	the solubilizer macrogolglycerol hydroxystearate	1290:1337	the solubilizer macrogolglycerol hydroxystearate	1290:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	16	theme	macrogolglycerol	1306:1321	arg1	polymers					1203:1210	The acidic polymers	1192:1210	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate	1192:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	4	17	from	buffer	854:859	arg1	dissolution					817:827	a direct dissolution	808:827	a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation	808:916	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	4	17	from	buffer	854:859	arg1	tests					653:657	Dissolution tests	641:657	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	641:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	2	18	theme	dissolved	472:480	arg1	API					482:484	dissolved API	472:484	dissolved API	472:484	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	4	19	theme	medium	692:697	arg1	change					666:671	a change	664:671	a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	664:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	7	20	theme	tests	1185:1189	arg1	types					1176:1180	both types	1171:1180	both types of tests	1171:1189	It was possible to identify the superior ASD-compositions for both types of tests.					
30261267	4	21	with	dissolution	817:827	arg1	change					666:671	a change	664:671	a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	664:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	8	22	theme	best	1350:1353	arg1	performances					1355:1366	the best performances	1346:1366	the best performances	1346:1366	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	0	23	theme	ketoconazole	89:100	arg1	behavior					77:84	recrystallization behavior	59:84	recrystallization behavior	59:84	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	0	23	theme	ketoconazole	89:100	arg1	dissolution					43:53	dissolution	43:53	dissolution	43:53	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	4	24	theme	pH	756:757	arg1	conditions					759:768	pH conditions	756:768	pH conditions for instant release formulations	756:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	4	25	from	dissolution	817:827	arg1	buffer					854:859	phosphate buffer	844:859	phosphate buffer pH 6.8	844:866	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	4	26	from	pH	737:738	arg1	buffer					727:732	phosphate buffer	717:732	phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	717:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	6	27	theme	Varying	1024:1030	arg1	behaviors					1044:1052	Varying dissolution behaviors	1024:1052	Varying dissolution behaviors between medium change and direct dissolution	1024:1097	Varying dissolution behaviors between medium change and direct dissolution occurred.					
30261267	2	28	theme	polymers	438:445	arg1	presence					399:406	the presence	395:406	the presence of crystallization-inhibiting polymers	395:445	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	2	28	theme	polymers	438:445	arg1	combination					345:355	The combination	341:355	The combination of an amorphous state of the drug	341:389	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	2	29	theme	amorphous	363:371	arg1	state					373:377	an amorphous state	360:377	an amorphous state of the drug	360:389	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	7	30	theme	superior	1141:1148	arg1	ASD-compositions					1150:1165	the superior ASD-compositions	1137:1165	the superior ASD-compositions for both types of tests	1137:1189	It was possible to identify the superior ASD-compositions for both types of tests.					
30261267	10	31	theme	formulations	1535:1546	arg1	optimization					1514:1525	The optimization	1510:1525	The optimization of such formulations with different release-patterns	1510:1578	The optimization of such formulations with different release-patterns plays an important role for the enhancement of the oral bioavailability of poorly water-soluble drugs.					
30261267	2	32	theme	drug	386:389	arg1	state					373:377	an amorphous state	360:377	an amorphous state of the drug	360:389	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	0	33	theme	excipients	25:34	arg1	influence					4:12	The influence	0:12	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.	0:168	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	4	34	with	tests	653:657	arg1	change					666:671	a change	664:671	a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	664:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	1	35	theme	active	299:304	arg1	APIs					334:337	APIs	334:337	APIs	334:337	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	1	35	theme	active	299:304	arg1	ingredients					321:331	active pharmaceutical ingredients	299:331	poorly water-soluble active pharmaceutical ingredients (APIs)	278:338	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	1	36	theme	ingredients	321:331	arg1	bioavailability					259:273	the bioavailability	255:273	the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs)	255:338	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	10	37	theme	different	1553:1561	arg1	release-patterns					1563:1578	different release-patterns	1553:1578	different release-patterns	1553:1578	The optimization of such formulations with different release-patterns plays an important role for the enhancement of the oral bioavailability of poorly water-soluble drugs.					
30261267	4	38	from	HCl	710:712	arg1	medium					692:697	the dissolution medium	676:697	the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	676:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	4	38	from	HCl	710:712	arg1	change					666:671	a change	664:671	a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	664:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	9	39	theme	acidic	1392:1397	arg1	polymers					1399:1406	the acidic polymers	1388:1406	the acidic polymers	1388:1406	The combination of the acidic polymers with macrogolglycerol hydroxystearate showed an improved dissolution behavior at higher API contents.					
30261267	4	40	theme	release	782:788	arg1	formulations					790:801	instant release formulations	774:801	instant release formulations	774:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	6	41	theme	medium	1062:1067	arg1	change					1069:1074	medium change	1062:1074	medium change	1062:1074	Varying dissolution behaviors between medium change and direct dissolution occurred.					
30261267	10	42	theme	important	1589:1597	arg1	role					1599:1602	an important role	1586:1602	an important role	1586:1602	The optimization of such formulations with different release-patterns plays an important role for the enhancement of the oral bioavailability of poorly water-soluble drugs.					
30261267	4	43	theme	ASDs	836:839	arg1	dissolution					817:827	a direct dissolution	808:827	a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation	808:916	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	4	43	theme	ASDs	836:839	arg1	tests					653:657	Dissolution tests	641:657	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	641:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	3	44	with	ASDs	497:500	arg1	ketoconazole					507:518	ketoconazole	507:518	ketoconazole	507:518	ASDs with ketoconazole and different polymers were manufactured by spray drying and their characteristics as well as performance were analyzed.					
30261267	3	44	with	ASDs	497:500	arg1	polymers					534:541	different polymers	524:541	different polymers	524:541	ASDs with ketoconazole and different polymers were manufactured by spray drying and their characteristics as well as performance were analyzed.					
30261267	3	45	theme	different	524:532	arg1	polymers					534:541	different polymers	524:541	different polymers	524:541	ASDs with ketoconazole and different polymers were manufactured by spray drying and their characteristics as well as performance were analyzed.					
30261267	4	46	theme	phosphate	844:852	arg1	buffer					854:859	phosphate buffer	844:859	phosphate buffer pH 6.8	844:866	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	9	47	with	combination	1373:1383	arg1	hydroxystearate					1430:1444	macrogolglycerol hydroxystearate	1413:1444	macrogolglycerol hydroxystearate	1413:1444	The combination of the acidic polymers with macrogolglycerol hydroxystearate showed an improved dissolution behavior at higher API contents.					
30261267	9	48	theme	dissolution	1465:1475	arg1	behavior					1477:1484	an improved dissolution behavior	1453:1484	an improved dissolution behavior	1453:1484	The combination of the acidic polymers with macrogolglycerol hydroxystearate showed an improved dissolution behavior at higher API contents.					
30261267	4	49	theme	direct	810:815	arg1	dissolution					817:827	a direct dissolution	808:827	a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation	808:916	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	8	50	theme	acidic	1196:1201	arg1	copolymer					1244:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	50	theme	acidic	1196:1201	arg1	hydroxystearate					1323:1337	the solubilizer macrogolglycerol hydroxystearate	1290:1337	the solubilizer macrogolglycerol hydroxystearate	1290:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	50	theme	acidic	1196:1201	arg1	polymers					1203:1210	The acidic polymers	1192:1210	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate	1192:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	50	theme	acidic	1196:1201	arg1	succinate					1276:1284	hypromellose acetate succinate	1255:1284	hypromellose acetate succinate	1255:1284	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	4	51	from	tests	653:657	arg1	buffer					854:859	phosphate buffer	844:859	phosphate buffer pH 6.8	844:866	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	8	52	theme	methacrylic	1212:1222	arg1	copolymer					1244:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	52	theme	methacrylic	1212:1222	arg1	polymers					1203:1210	The acidic polymers	1192:1210	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate	1192:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	10	53	theme	bioavailability	1636:1650	arg1	enhancement					1612:1622	the enhancement	1608:1622	the enhancement of the oral bioavailability of poorly water-soluble drugs	1608:1680	The optimization of such formulations with different release-patterns plays an important role for the enhancement of the oral bioavailability of poorly water-soluble drugs.					
30261267	9	54	theme	API	1496:1498	arg1	contents					1500:1507	higher API contents	1489:1507	higher API contents	1489:1507	The combination of the acidic polymers with macrogolglycerol hydroxystearate showed an improved dissolution behavior at higher API contents.					
30261267	8	55	theme	acrylate	1235:1242	arg1	copolymer					1244:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	55	theme	acrylate	1235:1242	arg1	polymers					1203:1210	The acidic polymers	1192:1210	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate	1192:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	5	56	with	ASDs	939:942	arg1	contents					953:960	API contents	949:960	API contents	949:960	All ASDs with API contents between 25 and 50% by weight were completely X-ray amorphous.					
30261267	1	57	theme	solid	199:203	arg1	ASDs					218:221	ASDs	218:221	ASDs	218:221	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	1	57	theme	solid	199:203	arg1	dispersions					205:215	amorphous solid dispersions	189:215	amorphous solid dispersions (ASDs)	189:222	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	10	58	theme	drugs	1676:1680	arg1	bioavailability					1636:1650	the oral bioavailability	1627:1650	the oral bioavailability of poorly water-soluble drugs	1627:1680	The optimization of such formulations with different release-patterns plays an important role for the enhancement of the oral bioavailability of poorly water-soluble drugs.					
30261267	8	59	theme	acetate	1268:1274	arg1	succinate					1276:1284	hypromellose acetate succinate	1255:1284	hypromellose acetate succinate	1255:1284	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	59	theme	acetate	1268:1274	arg1	polymers					1203:1210	The acidic polymers	1192:1210	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate	1192:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	0	60	theme	shift	156:160	arg1	method					162:167	a pH shift method	151:167	a pH shift method	151:167	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	10	61	with	optimization	1514:1525	arg1	release-patterns					1563:1578	different release-patterns	1553:1578	different release-patterns	1553:1578	The optimization of such formulations with different release-patterns plays an important role for the enhancement of the oral bioavailability of poorly water-soluble drugs.					
30261267	0	62	theme	recrystallization	59:75	arg1	behavior					77:84	recrystallization behavior	59:84	recrystallization behavior	59:84	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	2	63	theme	API	482:484	arg1	API					482:484	dissolved API	472:484	dissolved API	472:484	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	2	63	theme	API	482:484	arg1	amount					462:467	a high amount	455:467	a high amount of dissolved API	455:484	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	8	64	theme	solubilizer	1294:1304	arg1	hydroxystearate					1323:1337	the solubilizer macrogolglycerol hydroxystearate	1290:1337	the solubilizer macrogolglycerol hydroxystearate	1290:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	64	theme	solubilizer	1294:1304	arg1	polymers					1203:1210	The acidic polymers	1192:1210	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate	1192:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	0	65	from	influence	4:12	arg1	behavior					77:84	recrystallization behavior	59:84	recrystallization behavior	59:84	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	0	65	from	influence	4:12	arg1	dissolution					43:53	dissolution	43:53	dissolution	43:53	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	4	66	theme	dissolution	680:690	arg1	medium					692:697	the dissolution medium	676:697	the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	676:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	4	67	from	ASDs	836:839	arg1	buffer					854:859	phosphate buffer	844:859	phosphate buffer pH 6.8	844:866	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	6	68	theme	dissolution	1032:1042	arg1	behaviors					1044:1052	Varying dissolution behaviors	1024:1052	Varying dissolution behaviors between medium change and direct dissolution	1024:1097	Varying dissolution behaviors between medium change and direct dissolution occurred.					
30261267	2	69	theme	state	373:377	arg1	presence					399:406	the presence	395:406	the presence of crystallization-inhibiting polymers	395:445	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	2	69	theme	state	373:377	arg1	combination					345:355	The combination	341:355	The combination of an amorphous state of the drug	341:389	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	2	70	theme	crystallization-inhibiting	411:436	arg1	polymers					438:445	crystallization-inhibiting polymers	411:445	crystallization-inhibiting polymers	411:445	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	4	71	theme	phosphate	717:725	arg1	buffer					727:732	phosphate buffer	717:732	phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations	717:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	0	72	theme	polymer	17:23	arg1	excipients					25:34	polymer excipients	17:34	polymer excipients	17:34	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	8	73	dep	polymers	1203:1210	arg1	copolymer					1244:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	methacrylic acid-ethyl acrylate copolymer	1212:1252	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	73	dep	polymers	1203:1210	arg1	hydroxystearate					1323:1337	the solubilizer macrogolglycerol hydroxystearate	1290:1337	the solubilizer macrogolglycerol hydroxystearate	1290:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	73	dep	polymers	1203:1210	arg1	succinate					1276:1284	hypromellose acetate succinate	1255:1284	hypromellose acetate succinate	1255:1284	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	73	dep	polymers	1203:1210	arg1	polymers					1203:1210	The acidic polymers	1192:1210	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate	1192:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	10	74	theme	such	1530:1533	arg1	formulations					1535:1546	such formulations	1530:1546	such formulations	1530:1546	The optimization of such formulations with different release-patterns plays an important role for the enhancement of the oral bioavailability of poorly water-soluble drugs.					
30261267	4	75	dep	buffer	854:859	arg1	pH					861:862	pH 6.8	861:866	phosphate buffer pH 6.8	844:866	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	8	76	theme	hypromellose	1255:1266	arg1	succinate					1276:1284	hypromellose acetate succinate	1255:1284	hypromellose acetate succinate	1255:1284	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	8	76	theme	hypromellose	1255:1266	arg1	polymers					1203:1210	The acidic polymers	1192:1210	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate	1192:1337	The acidic polymers methacrylic acid-ethyl acrylate copolymer, hypromellose acetate succinate and the solubilizer macrogolglycerol hydroxystearate showed the best performances.					
30261267	0	77	dep	dissolution	43:53	arg1	the					39:41	the	39:41	the	39:41	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	2	78	theme	high	457:460	arg1	API					482:484	dissolved API	472:484	dissolved API	472:484	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	2	78	theme	high	457:460	arg1	amount					462:467	a high amount	455:467	a high amount of dissolved API	455:484	The combination of an amorphous state of the drug and the presence of crystallization-inhibiting polymers retains a high amount of dissolved API over time.					
30261267	1	79	theme	water-soluble	285:297	arg1	APIs					334:337	APIs	334:337	APIs	334:337	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	1	79	theme	water-soluble	285:297	arg1	ingredients					321:331	active pharmaceutical ingredients	299:331	poorly water-soluble active pharmaceutical ingredients (APIs)	278:338	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	1	80	theme	pharmaceutical	306:319	arg1	APIs					334:337	APIs	334:337	APIs	334:337	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	1	80	theme	pharmaceutical	306:319	arg1	ingredients					321:331	active pharmaceutical ingredients	299:331	poorly water-soluble active pharmaceutical ingredients (APIs)	278:338	The formulation of amorphous solid dispersions (ASDs) is an effective way to improve the bioavailability of poorly water-soluble active pharmaceutical ingredients (APIs).					
30261267	5	81	theme	API	949:951	arg1	contents					953:960	API contents	949:960	API contents	949:960	All ASDs with API contents between 25 and 50% by weight were completely X-ray amorphous.					
30261267	6	82	theme	direct	1080:1085	arg1	dissolution					1087:1097	direct dissolution	1080:1097	direct dissolution	1080:1097	Varying dissolution behaviors between medium change and direct dissolution occurred.					
30261267	9	83	theme	polymers	1399:1406	arg1	combination					1373:1383	The combination	1369:1383	The combination of the acidic polymers with macrogolglycerol hydroxystearate	1369:1444	The combination of the acidic polymers with macrogolglycerol hydroxystearate showed an improved dissolution behavior at higher API contents.					
30261267	0	84	dep	influence	4:12	arg1	variation					116:124	variation	116:124	variation	116:124	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	0	84	dep	influence	4:12	arg1	aspects					140:146	practical aspects	130:146	practical aspects of a pH shift method	130:167	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	0	84	dep	influence	4:12	arg1	Application					103:113	Application	103:113	Application	103:113	The influence of polymer excipients on the dissolution and recrystallization behavior of ketoconazole: Application, variation and practical aspects of a pH shift method.					
30261267	4	85	theme	instant	774:780	arg1	formulations					790:801	instant release formulations	774:801	instant release formulations	774:801	Dissolution tests with a change of the dissolution medium from 0.1 M HCl to phosphate buffer at pH 6.8 to simulate pH conditions for instant release formulations, and a direct dissolution of the ASDs in phosphate buffer pH 6.8 to simulate conditions for an enteric formulation, were performed.					
30261267	9	86	theme	macrogolglycerol	1413:1428	arg1	hydroxystearate					1430:1444	macrogolglycerol hydroxystearate	1413:1444	macrogolglycerol hydroxystearate	1413:1444	The combination of the acidic polymers with macrogolglycerol hydroxystearate showed an improved dissolution behavior at higher API contents.					
31387210	7	0	dep	in	1110:1111	arg1	vitro					1113:1117	vitro	1113:1117	vitro	1113:1117	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	4	1	dep	in	691:692	arg1	vitro					694:698	vitro	694:698	vitro	694:698	3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model.					
31387210	5	2	theme	cell	800:803	arg1	types					805:809	three different cell types	784:809	three different cell types	784:809	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	3	3	theme	every	471:475	arg1	types					482:486	almost every cell types	464:486	almost every cell types	464:486	Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs.					
31387210	7	4	theme	printed	1050:1056	arg1	shape					1058:1062	the printed shape	1046:1062	the printed shape	1046:1062	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	3	5	theme	pluripotent	357:367	arg1	cells					374:378	Induced pluripotent stem cells	349:378	Induced pluripotent stem cells (iPSCs)	349:386	Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs.					
31387210	3	5	theme	pluripotent	357:367	arg1	iPSCs					381:385	iPSCs	381:385	iPSCs	381:385	Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs.					
31387210	4	6	theme	in	691:692	arg1	model					700:704	a realistic in vitro model	679:704	a realistic in vitro model	679:704	3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model.					
31387210	3	7	theme	stem	369:372	arg1	cells					374:378	Induced pluripotent stem cells	349:378	Induced pluripotent stem cells (iPSCs)	349:386	Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs.					
31387210	3	7	theme	stem	369:372	arg1	iPSCs					381:385	iPSCs	381:385	iPSCs	381:385	Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs.					
31387210	7	8	theme	shape	1058:1062	arg1	maintenance					1031:1041	the maintenance	1027:1041	the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology	1027:1177	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	4	9	theme	realistic	681:689	arg1	model					700:704	a realistic in vitro model	679:704	a realistic in vitro model	679:704	3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model.					
31387210	2	10	theme	NDs	253:255	arg1	study					244:248	the study	240:248	the study of NDs	240:255	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	5	11	theme	cell	828:831	arg1	SH-SY5Y					839:845	SH-SY5Y	839:845	SH-SY5Y	839:845	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	11	theme	cell	828:831	arg1	line					833:836	a neuroblastoma cell line	812:836	a neuroblastoma cell line (SH-SY5Y)	812:846	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	11	theme	cell	828:831	arg1	biomaterials					737:748	two biomaterials	733:748	two biomaterials: sodium alginate and gelatin	733:777	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	7	12	theme	neural	1119:1124	arg1	tissue					1126:1131	a reliable in vitro neural tissue	1099:1131	a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology	1099:1177	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	4	13	theme	model	700:704	arg1	creation					667:674	the creation	663:674	the creation of a realistic in vitro model	663:704	3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model.					
31387210	5	14	theme	neuroblastoma	814:826	arg1	SH-SY5Y					839:845	SH-SY5Y	839:845	SH-SY5Y	839:845	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	14	theme	neuroblastoma	814:826	arg1	line					833:836	a neuroblastoma cell line	812:836	a neuroblastoma cell line (SH-SY5Y)	812:846	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	14	theme	neuroblastoma	814:826	arg1	biomaterials					737:748	two biomaterials	733:748	two biomaterials: sodium alginate and gelatin	733:777	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	1	15	theme	Neurodegenerative	74:90	arg1	class					119:123	a broad class	111:123	a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system	111:217	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	1	15	theme	Neurodegenerative	74:90	arg1	NDs					102:104	NDs	102:104	NDs	102:104	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	1	15	theme	Neurodegenerative	74:90	arg1	diseases					92:99	Neurodegenerative diseases	74:99	Neurodegenerative diseases (NDs)	74:105	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	1	16	theme	progressive	161:171	arg1	loss					173:176	the progressive loss	157:176	the progressive loss of neurons in the central nervous system	157:217	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	3	17	theme	Induced	349:355	arg1	cells					374:378	Induced pluripotent stem cells	349:378	Induced pluripotent stem cells (iPSCs)	349:386	Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs.					
31387210	3	17	theme	Induced	349:355	arg1	iPSCs					381:385	iPSCs	381:385	iPSCs	381:385	Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs.					
31387210	7	18	theme	iPSCs	1162:1166	arg1	technology					1168:1177	3D bioprinting and iPSCs technology	1143:1177	technology	1168:1177	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	0	19	theme	Bioink	0:5	arg1	Composition					7:17	Bioink Composition	0:17	Bioink Composition	0:17	Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue.					
31387210	2	20	from	problem	229:235	arg1	study					244:248	the study	240:248	the study of NDs	240:255	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	0	21	theme	Printing	23:30	arg1	Parameters					32:41	Printing Parameters	23:41	Printing Parameters	23:41	Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue.					
31387210	1	22	theme	neurons	181:187	arg1	loss					173:176	the progressive loss	157:176	the progressive loss of neurons in the central nervous system	157:217	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	6	23	theme	good	993:996	arg1	viability					998:1006	good viability	993:1006	good viability	993:1006	All cells were encapsulated inside the bioink, printed and cultivated for at least seven days; they all presented good viability.					
31387210	2	24	theme	pathogenic	326:335	arg1	mechanisms					337:346	the pathogenic mechanisms	322:346	the pathogenic mechanisms	322:346	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	7	25	theme	3D	1143:1144	arg1	bioprinting					1146:1156	3D bioprinting and iPSCs technology	1143:1177	bioprinting	1146:1156	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	5	26	theme	sodium	751:756	arg1	biomaterials					737:748	two biomaterials	733:748	two biomaterials: sodium alginate and gelatin	733:777	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	26	theme	sodium	751:756	arg1	alginate					758:765	sodium alginate	751:765	sodium alginate	751:765	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	4	27	theme	cells	629:633	arg1	distribution					613:624	the distribution	609:624	the distribution of cells such as neurons	609:649	3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model.					
31387210	1	28	theme	central	196:202	arg1	system					212:217	the central nervous system	192:217	the central nervous system	192:217	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	0	29	theme	Modeling	50:57	arg1	Tissue					66:71	3D Modeling Neural Tissue	47:71	3D Modeling Neural Tissue	47:71	Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue.					
31387210	7	30	theme	degenerative	1208:1219	arg1	unknown					1253:1259	unknown	1253:1259	unknown	1253:1259	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	7	30	theme	degenerative	1208:1219	arg1	processes					1221:1229	the degenerative processes	1204:1229	the degenerative processes that are still widely unknown	1204:1259	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	2	31	theme	model	307:311	arg1	lack					264:267	the lack	260:267	the lack of an adequate realistic experimental model to study the pathogenic mechanisms	260:346	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	2	31	theme	model	307:311	arg1	problem					229:235	The main problem	220:235	The main problem in the study of NDs	220:255	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	0	32	theme	3D	47:48	arg1	Tissue					66:71	3D Modeling Neural Tissue	47:71	3D Modeling Neural Tissue	47:71	Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue.					
31387210	7	33	theme	processes	1221:1229	arg1	study					1195:1199	the study	1191:1199	the study of the degenerative processes that are still widely unknown	1191:1259	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	2	34	theme	experimental	294:305	arg1	model					307:311	an adequate realistic experimental model	272:311	an adequate realistic experimental model to study the pathogenic mechanisms	272:346	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	7	35	theme	reliable	1101:1108	arg1	tissue					1126:1131	a reliable in vitro neural tissue	1099:1131	a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology	1099:1177	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	5	36	theme	neural	860:865	arg1	cells					872:876	neural stem cells	860:876	neural stem cells	860:876	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	1	37	theme	broad	113:117	arg1	class					119:123	a broad class	111:123	a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system	111:217	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	1	37	theme	broad	113:117	arg1	diseases					92:99	Neurodegenerative diseases	74:99	Neurodegenerative diseases (NDs)	74:105	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	1	38	theme	nervous	204:210	arg1	system					212:217	the central nervous system	192:217	the central nervous system	192:217	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	7	39	dep	shape	1058:1062	arg1	opening					1065:1071	opening	1065:1071	opening	1065:1071	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	5	40	theme	stem	867:870	arg1	cells					872:876	neural stem cells	860:876	neural stem cells	860:876	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	1	41	from	loss	173:176	arg1	system					212:217	the central nervous system	192:217	the central nervous system	192:217	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	2	42	theme	adequate	275:282	arg1	model					307:311	an adequate realistic experimental model	272:311	an adequate realistic experimental model to study the pathogenic mechanisms	272:346	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	2	43	theme	realistic	284:292	arg1	model					307:311	an adequate realistic experimental model	272:311	an adequate realistic experimental model to study the pathogenic mechanisms	272:346	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	5	44	theme	different	790:798	arg1	types					805:809	three different cell types	784:809	three different cell types	784:809	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	45	dep	biomaterials	737:748	arg1	cells					872:876	neural stem cells	860:876	neural stem cells	860:876	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	45	dep	biomaterials	737:748	arg1	biomaterials					737:748	two biomaterials	733:748	two biomaterials: sodium alginate and gelatin	733:777	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	45	dep	biomaterials	737:748	arg1	iPSCs					849:853	iPSCs	849:853	iPSCs	849:853	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	45	dep	biomaterials	737:748	arg1	alginate					758:765	sodium alginate	751:765	sodium alginate	751:765	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	45	dep	biomaterials	737:748	arg1	line					833:836	a neuroblastoma cell line	812:836	a neuroblastoma cell line (SH-SY5Y)	812:846	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	45	dep	biomaterials	737:748	arg1	SH-SY5Y					839:845	SH-SY5Y	839:845	SH-SY5Y	839:845	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	5	45	dep	biomaterials	737:748	arg1	gelatin					771:777	gelatin	771:777	gelatin	771:777	In this work, we analyzed two biomaterials: sodium alginate and gelatin, and three different cell types: a neuroblastoma cell line (SH-SY5Y), iPSCs, and neural stem cells.					
31387210	4	46	theme	3D	576:577	arg1	bioprinting					579:589	3D bioprinting	576:589	3D bioprinting	576:589	3D bioprinting allows to control the distribution of cells such as neurons, leading to the creation of a realistic in vitro model.					
31387210	3	47	theme	cell	477:480	arg1	types					482:486	almost every cell types	464:486	almost every cell types	464:486	Induced pluripotent stem cells (iPSCs) partially overcome the problem, with their capability to differentiate into almost every cell types; even so, these cells alone are not sufficient to unveil the mechanisms underlying NDs.					
31387210	0	48	theme	Neural	59:64	arg1	Tissue					66:71	3D Modeling Neural Tissue	47:71	3D Modeling Neural Tissue	47:71	Bioink Composition and Printing Parameters for 3D Modeling Neural Tissue.					
31387210	2	49	theme	main	224:227	arg1	problem					229:235	The main problem	220:235	The main problem in the study of NDs	220:255	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	2	49	theme	main	224:227	arg1	lack					264:267	the lack	260:267	the lack of an adequate realistic experimental model to study the pathogenic mechanisms	260:346	The main problem in the study of NDs is the lack of an adequate realistic experimental model to study the pathogenic mechanisms.					
31387210	7	50	theme	in	1110:1111	arg1	tissue					1126:1131	a reliable in vitro neural tissue	1099:1131	a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology	1099:1177	We also evaluated the maintenance of the printed shape, opening the possibility to obtain a reliable in vitro neural tissue combining 3D bioprinting and iPSCs technology, optimizing the study of the degenerative processes that are still widely unknown.					
31387210	1	51	theme	pathologies	128:138	arg1	class					119:123	a broad class	111:123	a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system	111:217	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31387210	1	51	theme	pathologies	128:138	arg1	diseases					92:99	Neurodegenerative diseases	74:99	Neurodegenerative diseases (NDs)	74:105	Neurodegenerative diseases (NDs) are a broad class of pathologies characterized by the progressive loss of neurons in the central nervous system.					
31079452	8	0	theme	unique	1640:1645	arg1	N-glycopeptides					1647:1661	more than 500 unique N-glycopeptides	1626:1661	more than 500 unique N-glycopeptides	1626:1661	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	4	1	theme	peptide	1113:1119	arg1	backbone					1121:1128	the same peptide backbone	1104:1128	the same peptide backbone	1104:1128	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	3	2	theme	theoretical	746:756	arg1	approach					772:779	a graph theoretical bioinformatic approach	738:779	a graph theoretical bioinformatic approach complementary to the database-dependent method	738:826	To address these challenges, we present GlycopeptideGraphMS, a graph theoretical bioinformatic approach complementary to the database-dependent method.					
31079452	3	2	theme	theoretical	746:756	arg1	GlycopeptideGraphMS					717:735	GlycopeptideGraphMS	717:735	GlycopeptideGraphMS	717:735	To address these challenges, we present GlycopeptideGraphMS, a graph theoretical bioinformatic approach complementary to the database-dependent method.					
31079452	1	3	gly	N-glycosylated	186:199	arg1	peptides					201:208	N-glycosylated peptides	186:208	N-glycosylated peptides	186:208	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	8	4	theme	protease	1796:1803	arg1	cleavage					1805:1812	a nonspecific protease cleavage	1782:1812	a nonspecific protease cleavage	1782:1812	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	4	5	theme	LCMS	954:957	arg1	features					959:966	those LCMS features	948:966	those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences	948:1067	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	7	6	theme	experimental	1531:1542	arg1	rate					1560:1563	the experimental false discovery rate	1527:1563	the experimental false discovery rate of identifications	1527:1582	By validating the identifications with exoglycosidase cocktails and MS/MS fragmentation, we determined the experimental false discovery rate of identifications to be 2.21%.					
31079452	6	7	gly	N-glycopeptide	1323:1336	arg2	N-glycopeptide					1323:1336	just one N-glycopeptide	1314:1336	just one N-glycopeptide	1314:1336	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	3	8	theme	database-dependent	802:819	arg1	method					821:826	the database-dependent method	798:826	the database-dependent method	798:826	To address these challenges, we present GlycopeptideGraphMS, a graph theoretical bioinformatic approach complementary to the database-dependent method.					
31079452	8	9	with	search	1711:1716	arg1	software					1730:1737	Byonic software	1723:1737	Byonic software	1723:1737	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	6	10	from	graph	1345:1349	arg1	sequencing					1262:1271	MS/MS sequencing	1256:1271	MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph	1256:1349	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	7	11	theme	identifications	1568:1582	arg1	rate					1560:1563	the experimental false discovery rate	1527:1563	the experimental false discovery rate of identifications	1527:1582	By validating the identifications with exoglycosidase cocktails and MS/MS fragmentation, we determined the experimental false discovery rate of identifications to be 2.21%.					
31079452	4	12	with	N-glycopeptides	1083:1097	arg1	backbone					1121:1128	the same peptide backbone	1104:1128	the same peptide backbone	1104:1128	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	9	13	theme	comprehensive	1903:1915	arg1	glycoproteomics					1927:1941	comprehensive automated glycoproteomics	1903:1941	comprehensive automated glycoproteomics	1903:1941	This method overcomes some limitations of the database approach and is a step closer to comprehensive automated glycoproteomics.					
31079452	6	14	gly	N-glycopeptides	1394:1408	arg2	N-glycopeptides					1394:1408	the N-glycopeptides	1390:1408	the N-glycopeptides in the graph	1390:1421	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	1	15	theme	liquid	213:218	arg1	method					163:168	The leading proteomic method	141:168	The leading proteomic method for identifying N-glycosylated peptides	141:208	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	1	15	theme	liquid	213:218	arg1	chromatography					220:233	liquid chromatography	213:233	liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations	213:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	2	16	theme	glycan	618:623	arg1	combinations					625:636	some glycan combinations	613:636	some glycan combinations	613:636	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	7	17	theme	MS/MS	1492:1496	arg1	fragmentation					1498:1510	MS/MS fragmentation	1492:1510	MS/MS fragmentation	1492:1510	By validating the identifications with exoglycosidase cocktails and MS/MS fragmentation, we determined the experimental false discovery rate of identifications to be 2.21%.					
31079452	6	18	theme	N-glycopeptide	1323:1336	arg1	composition					1299:1309	the glycan and peptide composition	1276:1309	composition	1299:1309	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	2	19	theme	high-quality	483:494	arg1	spectra					514:520	high-quality informative MS/MS spectra	483:520	high-quality informative MS/MS spectra	483:520	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	1	20	theme	tandem	248:253	arg1	spectrometry					274:285	tandem fragmentation mass spectrometry	248:285	tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations	248:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	1	20	theme	tandem	248:253	arg1	LCMS/MS					288:294	LCMS/MS	288:294	LCMS/MS	288:294	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	6	21	theme	composition	1299:1309	arg1	sequencing					1262:1271	MS/MS sequencing	1256:1271	MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph	1256:1349	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	1	22	theme	leading	145:151	arg1	method					163:168	The leading proteomic method	141:168	The leading proteomic method for identifying N-glycosylated peptides	141:208	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	1	22	theme	leading	145:151	arg1	chromatography					220:233	liquid chromatography	213:233	liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations	213:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	4	23	theme	mass	1033:1036	arg1	differences					1057:1067	glycan mass and retention time differences	1026:1067	glycan mass and retention time differences	1026:1067	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	9	24	theme	database	1861:1868	arg1	approach					1870:1877	the database approach	1857:1877	the database approach	1857:1877	This method overcomes some limitations of the database approach and is a step closer to comprehensive automated glycoproteomics.					
31079452	4	25	with	glycoprotein	885:896	arg1	sites					928:932	multiple N-glycosylation sites	903:932	multiple N-glycosylation sites	903:932	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	26	theme	retention	1042:1050	arg1	time					1052:1055	retention time	1042:1055	retention time	1042:1055	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	0	27	theme	Theoretical	92:102	arg1	Patterns					104:111	Graph Theoretical Patterns	86:111	Graph Theoretical Patterns in Mass and Retention Time	86:138	GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time.					
31079452	1	28	theme	possible	369:376	arg1	glycan					378:383	possible glycan and peptide combinations	369:408	glycan	378:383	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	8	29	gly	N-glycopeptides	1647:1661	arg2	N-glycopeptides					1647:1661	more than 500 unique N-glycopeptides	1626:1661	more than 500 unique N-glycopeptides	1626:1661	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	2	30	theme	incorrect	583:591	arg1	assignments					593:603	incorrect assignments	583:603	incorrect assignments because some glycan combinations are equivalent in mass to amino acids	583:674	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	4	31	theme	tyrosine	852:859	arg1	AXL					869:871	AXL	869:871	AXL	869:871	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	31	theme	tyrosine	852:859	arg1	kinase					861:866	receptor tyrosine kinase	843:866	the AXL receptor tyrosine kinase (AXL)	835:872	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	1	32	theme	combinations	397:408	arg1	database					357:364	a database	355:364	a database of possible glycan and peptide combinations	355:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	2	33	theme	MS/MS	508:512	arg1	spectra					514:520	high-quality informative MS/MS spectra	483:520	high-quality informative MS/MS spectra	483:520	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	1	34	theme	MS/MS	330:334	arg1	masses					345:350	MS/MS fragment masses	330:350	MS/MS fragment masses	330:350	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	2	35	theme	database-dependent	416:433	arg1	approaches					435:444	Such database-dependent approaches	411:444	Such database-dependent approaches	411:444	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	4	36	gly	glycoprotein	885:896	arg1	glycoprotein					885:896	a model glycoprotein	877:896	a model glycoprotein with multiple N-glycosylation sites	877:932	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	36	gly	glycoprotein	885:896	arg1	AXL					839:841	the AXL receptor tyrosine kinase (AXL)	835:872	the AXL receptor tyrosine kinase (AXL)	835:872	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	37	theme	same	1108:1111	arg1	backbone					1121:1128	the same peptide backbone	1104:1128	the same peptide backbone	1104:1128	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	2	38	from	mass	656:659	arg1	equivalent					642:651	equivalent	642:651	equivalent	642:651	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	9	39	theme	automated	1917:1925	arg1	glycoproteomics					1927:1941	comprehensive automated glycoproteomics	1903:1941	comprehensive automated glycoproteomics	1903:1941	This method overcomes some limitations of the database approach and is a step closer to comprehensive automated glycoproteomics.					
31079452	0	40	theme	Improved	21:28	arg1	Detection					43:51	Improved Glycopeptide Detection	21:51	Improved Glycopeptide Detection	21:51	GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time.					
31079452	2	41	theme	unexpected	532:541	arg1	glycan					543:548	unexpected glycan or peptide sequences	532:569	glycan	543:548	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	1	42	theme	fragment	336:343	arg1	masses					345:350	MS/MS fragment masses	330:350	MS/MS fragment masses	330:350	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	2	43	theme	peptide	553:559	arg1	sequences					561:569	unexpected glycan or peptide sequences	532:569	sequences	561:569	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	4	44	theme	multiple	903:910	arg1	sites					928:932	multiple N-glycosylation sites	903:932	multiple N-glycosylation sites	903:932	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	6	45	from	N-glycopeptides	1394:1408	arg1	graph					1417:1421	the graph	1413:1421	the graph	1413:1421	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	3	46	theme	graph	740:744	arg1	approach					772:779	a graph theoretical bioinformatic approach	738:779	a graph theoretical bioinformatic approach complementary to the database-dependent method	738:826	To address these challenges, we present GlycopeptideGraphMS, a graph theoretical bioinformatic approach complementary to the database-dependent method.					
31079452	3	46	theme	graph	740:744	arg1	GlycopeptideGraphMS					717:735	GlycopeptideGraphMS	717:735	GlycopeptideGraphMS	717:735	To address these challenges, we present GlycopeptideGraphMS, a graph theoretical bioinformatic approach complementary to the database-dependent method.					
31079452	1	47	theme	fragmentation	255:267	arg1	spectrometry					274:285	tandem fragmentation mass spectrometry	248:285	tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations	248:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	1	47	theme	fragmentation	255:267	arg1	LCMS/MS					288:294	LCMS/MS	288:294	LCMS/MS	288:294	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	0	48	dep	GlycopeptideGraphMS	0:18	arg1	Identification					57:70	Identification	57:70	Identification	57:70	GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time.					
31079452	0	48	dep	GlycopeptideGraphMS	0:18	arg1	Detection					43:51	Improved Glycopeptide Detection	21:51	Improved Glycopeptide Detection	21:51	GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time.					
31079452	6	49	from	rest	1382:1385	arg1	graph					1417:1421	the graph	1413:1421	the graph	1413:1421	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	3	50	theme	complementary	781:793	arg1	approach					772:779	a graph theoretical bioinformatic approach	738:779	a graph theoretical bioinformatic approach complementary to the database-dependent method	738:826	To address these challenges, we present GlycopeptideGraphMS, a graph theoretical bioinformatic approach complementary to the database-dependent method.					
31079452	3	50	theme	complementary	781:793	arg1	GlycopeptideGraphMS					717:735	GlycopeptideGraphMS	717:735	GlycopeptideGraphMS	717:735	To address these challenges, we present GlycopeptideGraphMS, a graph theoretical bioinformatic approach complementary to the database-dependent method.					
31079452	2	51	theme	amino	664:668	arg1	acids					670:674	amino acids	664:674	amino acids	664:674	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	9	52	theme	approach	1870:1877	arg1	limitations					1842:1852	some limitations	1837:1852	some limitations of the database approach	1837:1877	This method overcomes some limitations of the database approach and is a step closer to comprehensive automated glycoproteomics.					
31079452	4	53	theme	different	1134:1142	arg1	compositions					1153:1164	different N-glycan compositions	1134:1164	different N-glycan compositions	1134:1164	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	8	54	theme	Byonic	1723:1728	arg1	software					1730:1737	Byonic software	1723:1737	Byonic software	1723:1737	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	3	55	theme	bioinformatic	758:770	arg1	approach					772:779	a graph theoretical bioinformatic approach	738:779	a graph theoretical bioinformatic approach complementary to the database-dependent method	738:826	To address these challenges, we present GlycopeptideGraphMS, a graph theoretical bioinformatic approach complementary to the database-dependent method.					
31079452	3	55	theme	bioinformatic	758:770	arg1	GlycopeptideGraphMS					717:735	GlycopeptideGraphMS	717:735	GlycopeptideGraphMS	717:735	To address these challenges, we present GlycopeptideGraphMS, a graph theoretical bioinformatic approach complementary to the database-dependent method.					
31079452	4	56	dep	differences	1057:1067	arg1	basis					1017:1021	basis	1017:1021	basis	1017:1021	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	56	dep	differences	1057:1067	arg1	the					1013:1015	the	1013:1015	the	1013:1015	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	8	57	theme	nonspecific	1784:1794	arg1	cleavage					1805:1812	a nonspecific protease cleavage	1782:1812	a nonspecific protease cleavage	1782:1812	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	2	58	dep	assignments	593:603	arg1	equivalent					642:651	equivalent	642:651	equivalent	642:651	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	7	59	theme	false	1544:1548	arg1	rate					1560:1563	the experimental false discovery rate	1527:1563	the experimental false discovery rate of identifications	1527:1582	By validating the identifications with exoglycosidase cocktails and MS/MS fragmentation, we determined the experimental false discovery rate of identifications to be 2.21%.					
31079452	2	60	from	equivalent	642:651	arg1	mass					656:659	mass	656:659	mass to amino acids	656:674	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	1	61	theme	N-glycosylated	186:199	arg1	peptides					201:208	N-glycosylated peptides	186:208	N-glycosylated peptides	186:208	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	7	62	theme	discovery	1550:1558	arg1	rate					1560:1563	the experimental false discovery rate	1527:1563	the experimental false discovery rate of identifications	1527:1582	By validating the identifications with exoglycosidase cocktails and MS/MS fragmentation, we determined the experimental false discovery rate of identifications to be 2.21%.					
31079452	4	63	theme	N-glycosylation	912:926	arg1	sites					928:932	multiple N-glycosylation sites	903:932	multiple N-glycosylation sites	903:932	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	6	64	theme	MS/MS	1256:1260	arg1	sequencing					1262:1271	MS/MS sequencing	1256:1271	MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph	1256:1349	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	7	65	with	identifications	1442:1456	arg1	cocktails					1478:1486	exoglycosidase cocktails	1463:1486	exoglycosidase cocktails	1463:1486	By validating the identifications with exoglycosidase cocktails and MS/MS fragmentation, we determined the experimental false discovery rate of identifications to be 2.21%.					
31079452	7	65	with	identifications	1442:1456	arg1	fragmentation					1498:1510	MS/MS fragmentation	1492:1510	MS/MS fragmentation	1492:1510	By validating the identifications with exoglycosidase cocktails and MS/MS fragmentation, we determined the experimental false discovery rate of identifications to be 2.21%.					
31079452	5	66	gly	unglycosylated	1179:1192	arg1	peptides					1194:1201	unglycosylated peptides	1179:1201	unglycosylated peptides	1179:1201	Conversely, unglycosylated peptides did not exhibit this grouping behavior.					
31079452	4	67	gly	N-glycosylation	912:926	arg2	sites					928:932	multiple N-glycosylation sites	903:932	multiple N-glycosylation sites	903:932	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	0	68	from	Patterns	104:111	arg1	Mass					116:119	Mass	116:119	Mass	116:119	GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time.					
31079452	0	68	from	Patterns	104:111	arg1	Time					135:138	Retention Time	125:138	Retention Time	125:138	GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time.					
31079452	4	69	theme	graph	995:999	arg1	networks					1001:1008	graph networks	995:1008	graph networks	995:1008	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	6	70	theme	N-glycopeptides	1394:1408	arg1	rest					1382:1385	the rest	1378:1385	the rest of the N-glycopeptides in the graph	1378:1421	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	4	71	dep	AXL	839:841	arg1	AXL					869:871	AXL	869:871	AXL	869:871	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	71	dep	AXL	839:841	arg1	kinase					861:866	receptor tyrosine kinase	843:866	the AXL receptor tyrosine kinase (AXL)	835:872	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	5	72	theme	grouping	1224:1231	arg1	behavior					1233:1240	this grouping behavior	1219:1240	this grouping behavior	1219:1240	Conversely, unglycosylated peptides did not exhibit this grouping behavior.					
31079452	7	73	theme	exoglycosidase	1463:1476	arg1	cocktails					1478:1486	exoglycosidase cocktails	1463:1486	exoglycosidase cocktails	1463:1486	By validating the identifications with exoglycosidase cocktails and MS/MS fragmentation, we determined the experimental false discovery rate of identifications to be 2.21%.					
31079452	6	74	theme	peptide	1291:1297	arg1	composition					1299:1309	the glycan and peptide composition	1276:1309	composition	1299:1309	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	0	75	theme	Graph	86:90	arg1	Patterns					104:111	Graph Theoretical Patterns	86:111	Graph Theoretical Patterns in Mass and Retention Time	86:138	GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time.					
31079452	1	76	theme	proteomic	153:161	arg1	method					163:168	The leading proteomic method	141:168	The leading proteomic method for identifying N-glycosylated peptides	141:208	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	1	76	theme	proteomic	153:161	arg1	chromatography					220:233	liquid chromatography	213:233	liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations	213:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	4	77	theme	glycan	1026:1031	arg1	mass					1033:1036	glycan mass	1026:1036	glycan mass	1026:1036	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	6	78	theme	glycan	1280:1285	arg1	composition					1299:1309	the glycan and peptide composition	1276:1309	composition	1299:1309	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	6	79	from	composition	1299:1309	arg1	graph					1345:1349	the graph	1341:1349	the graph	1341:1349	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	1	80	theme	glycan	378:383	arg1	database					357:364	a database	355:364	a database of possible glycan and peptide combinations	355:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	4	81	theme	receptor	843:850	arg1	AXL					869:871	AXL	869:871	AXL	869:871	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	81	theme	receptor	843:850	arg1	kinase					861:866	receptor tyrosine kinase	843:866	the AXL receptor tyrosine kinase (AXL)	835:872	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	6	82	from	sequencing	1262:1271	arg1	graph					1345:1349	the graph	1341:1349	the graph	1341:1349	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	0	83	theme	Retention	125:133	arg1	Time					135:138	Retention Time	125:138	Retention Time	125:138	GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time.					
31079452	1	84	theme	peptide	389:395	arg1	combinations					397:408	possible glycan and peptide combinations	369:408	combinations	397:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	9	85	theme	closer	1893:1898	arg1	step					1888:1891	a step	1886:1891	a step closer to comprehensive automated glycoproteomics	1886:1941	This method overcomes some limitations of the database approach and is a step closer to comprehensive automated glycoproteomics.					
31079452	5	86	theme	unglycosylated	1179:1192	arg1	peptides					1194:1201	unglycosylated peptides	1179:1201	unglycosylated peptides	1179:1201	Conversely, unglycosylated peptides did not exhibit this grouping behavior.					
31079452	2	87	theme	informative	496:506	arg1	spectra					514:520	high-quality informative MS/MS spectra	483:520	high-quality informative MS/MS spectra	483:520	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	2	88	theme	Such	411:414	arg1	approaches					435:444	Such database-dependent approaches	411:444	Such database-dependent approaches	411:444	Such database-dependent approaches come with challenges such as needing high-quality informative MS/MS spectra, ignoring unexpected glycan or peptide sequences, and making incorrect assignments because some glycan combinations are equivalent in mass to amino acids.					
31079452	0	89	theme	Glycopeptide	30:41	arg1	Detection					43:51	Improved Glycopeptide Detection	21:51	Improved Glycopeptide Detection	21:51	GlycopeptideGraphMS: Improved Glycopeptide Detection and Identification by Exploiting Graph Theoretical Patterns in Mass and Retention Time.					
31079452	1	90	theme	matching	318:325	arg1	spectral					309:316	spectral matching	309:325	spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations	309:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	8	91	theme	triple	1673:1678	arg1	number					1684:1689	triple the number	1673:1689	triple the number found by a database search with Byonic software	1673:1737	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	8	91	theme	triple	1673:1678	arg1	AXL					1668:1670	AXL	1668:1670	AXL	1668:1670	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	6	92	from	graph	1417:1421	arg1	rest					1382:1385	the rest	1378:1385	the rest of the N-glycopeptides in the graph	1378:1421	Furthermore, MS/MS sequencing of the glycan and peptide composition of just one N-glycopeptide in the graph was sufficient to identify the rest of the N-glycopeptides in the graph.					
31079452	4	93	gly	N-glycopeptides	1083:1097	arg2	N-glycopeptides					1083:1097	N-glycopeptides	1083:1097	N-glycopeptides with the same peptide backbone	1083:1128	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	94	theme	time	1052:1055	arg1	differences					1057:1067	glycan mass and retention time differences	1026:1067	glycan mass and retention time differences	1026:1067	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	95	theme	model	879:883	arg1	glycoprotein					885:896	a model glycoprotein	877:896	a model glycoprotein with multiple N-glycosylation sites	877:932	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	4	95	theme	model	879:883	arg1	AXL					839:841	the AXL receptor tyrosine kinase (AXL)	835:872	the AXL receptor tyrosine kinase (AXL)	835:872	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	1	96	theme	masses	345:350	arg1	spectral					309:316	spectral matching	309:325	spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations	309:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	8	97	theme	incorrect	1753:1761	arg1	assignments					1763:1773	incorrect assignments	1753:1773	incorrect assignments due to a nonspecific protease cleavage	1753:1812	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	4	98	theme	N-glycan	1144:1151	arg1	compositions					1153:1164	different N-glycan compositions	1134:1164	different N-glycan compositions	1134:1164	Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions.					
31079452	8	99	theme	database	1702:1709	arg1	search					1711:1716	a database search	1700:1716	a database search with Byonic software	1700:1737	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	8	100	theme	due	1775:1777	arg1	assignments					1763:1773	incorrect assignments	1753:1773	incorrect assignments due to a nonspecific protease cleavage	1753:1812	GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage.					
31079452	1	101	theme	mass	269:272	arg1	spectrometry					274:285	tandem fragmentation mass spectrometry	248:285	tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations	248:408	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31079452	1	101	theme	mass	269:272	arg1	LCMS/MS					288:294	LCMS/MS	288:294	LCMS/MS	288:294	The leading proteomic method for identifying N-glycosylated peptides is liquid chromatography coupled with tandem fragmentation mass spectrometry (LCMS/MS) followed by spectral matching of MS/MS fragment masses to a database of possible glycan and peptide combinations.					
31260766	5	0	theme	activity	947:954	arg1	rate					966:969	its activity retention rate	943:969	its activity retention rate	943:969	Unlike these four oligosaccharides, Chito-oligosaccharides (COS) changed the secondary and tertiary structure of β-Gal, thus decreasing its activity retention rate.					
31260766	0	1	theme	heat	81:84	arg1	treatment					86:94	heat treatment	81:94	heat treatment	81:94	Oligosaccharides act as the high efficiency stabilizer for β-galactosidase under heat treatment.					
31260766	5	2	theme	retention	956:964	arg1	rate					966:969	its activity retention rate	943:969	its activity retention rate	943:969	Unlike these four oligosaccharides, Chito-oligosaccharides (COS) changed the secondary and tertiary structure of β-Gal, thus decreasing its activity retention rate.					
31260766	6	3	dep	composition	1044:1054	arg1	w/v					1066:1068	w/v	1066:1068	w/v	1066:1068	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	6	3	dep	composition	1044:1054	arg1	IMO					1061:1063	30% IMO	1057:1063	30% IMO	1057:1063	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	6	3	dep	composition	1044:1054	arg1	XOS					1078:1080	40% XOS	1074:1080	40% XOS	1074:1080	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	7	4	theme	food	1361:1364	arg1	industry					1366:1373	food industry	1361:1373	food industry	1361:1373	This study provides an insight into the mechanism by which sugar stabilizes protein under heat stress and offers guidance for application of liquid lactase to food industry.					
31260766	6	5	theme	optimal	1036:1042	arg1	composition					1044:1054	optimal composition	1036:1054	optimal composition (30% IMO, w/v and 40% XOS, w/v)	1036:1086	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	2	6	theme	heat	286:289	arg1	stress					291:296	heat stress	286:296	heat stress	286:296	This study investigated the ability of oligosaccharides to protect β-Gal against heat stress.					
31260766	7	7	theme	heat	1292:1295	arg1	stress					1297:1302	heat stress	1292:1302	heat stress	1292:1302	This study provides an insight into the mechanism by which sugar stabilizes protein under heat stress and offers guidance for application of liquid lactase to food industry.					
31260766	6	8	theme	native	1151:1156	arg1	β-Gal					1158:1162	the native β-Gal	1147:1162	the native β-Gal (the activity retention rate of 20%)	1147:1199	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	5	9	theme	β-Gal	920:924	arg1	structure					907:915	the secondary and tertiary structure	880:915	the secondary and tertiary structure of β-Gal	880:924	Unlike these four oligosaccharides, Chito-oligosaccharides (COS) changed the secondary and tertiary structure of β-Gal, thus decreasing its activity retention rate.					
31260766	1	10	contain	has	143:145	arg1	β-Galactosidase					97:111	β-Galactosidase	97:111	β-Galactosidase (β-Gal) as dietary supplement	97:141	β-Galactosidase (β-Gal) as dietary supplement has the ability to alleviate symptoms of lactose intolerance.					
31260766	1	10	contain	has	143:145	arg2	ability					151:157	the ability to alleviate symptoms of lactose intolerance	147:202	the ability to alleviate symptoms of lactose intolerance	147:202	β-Galactosidase (β-Gal) as dietary supplement has the ability to alleviate symptoms of lactose intolerance.					
31260766	1	10	contain	has	143:145	arg1	β-Gal					114:118	β-Gal	114:118	β-Gal	114:118	β-Galactosidase (β-Gal) as dietary supplement has the ability to alleviate symptoms of lactose intolerance.					
31260766	4	11	theme	hydrogen	780:787	arg1	interaction					794:804	hydrogen bond interaction	780:804	hydrogen bond interaction	780:804	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	4	12	theme	secondary	705:713	arg1	structure					728:736	the secondary and tertiary structure	701:736	the secondary and tertiary structure of β-Gal	701:745	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	6	13	theme	β-Gal	1025:1029	arg1	rate					1017:1020	the activity retention rate	994:1020	the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v)	994:1086	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	1	14	theme	lactose	184:190	arg1	intolerance					192:202	lactose intolerance	184:202	lactose intolerance	184:202	β-Galactosidase (β-Gal) as dietary supplement has the ability to alleviate symptoms of lactose intolerance.					
31260766	1	15	theme	intolerance	192:202	arg1	symptoms					172:179	symptoms	172:179	symptoms of lactose intolerance	172:202	β-Galactosidase (β-Gal) as dietary supplement has the ability to alleviate symptoms of lactose intolerance.					
31260766	6	16	with	β-Gal	1025:1029	arg1	composition					1044:1054	optimal composition	1036:1054	optimal composition (30% IMO, w/v and 40% XOS, w/v)	1036:1086	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	4	17	theme	bond	789:792	arg1	interaction					794:804	hydrogen bond interaction	780:804	hydrogen bond interaction	780:804	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	6	18	theme	40	1074:1075	arg1	%					1076:1076	%	1076:1076	%	1076:1076	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	0	19	theme	efficiency	33:42	arg1	Oligosaccharides					0:15	Oligosaccharides	0:15	Oligosaccharides	0:15	Oligosaccharides act as the high efficiency stabilizer for β-galactosidase under heat treatment.					
31260766	0	19	theme	efficiency	33:42	arg1	stabilizer					44:53	the high efficiency stabilizer	24:53	the high efficiency stabilizer for β-galactosidase under heat treatment	24:94	Oligosaccharides act as the high efficiency stabilizer for β-galactosidase under heat treatment.					
31260766	6	20	theme	retention	1007:1015	arg1	rate					1017:1020	the activity retention rate	994:1020	the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v)	994:1086	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	4	21	dep	transform	606:614	arg1	infrared					616:623	infrared	616:623	transform infrared spectroscopy (FTIR)	606:643	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	7	22	theme	liquid	1343:1348	arg1	lactase					1350:1356	liquid lactase	1343:1356	liquid lactase	1343:1356	This study provides an insight into the mechanism by which sugar stabilizes protein under heat stress and offers guidance for application of liquid lactase to food industry.					
31260766	5	23	theme	tertiary	898:905	arg1	structure					907:915	the secondary and tertiary structure	880:915	the secondary and tertiary structure of β-Gal	880:924	Unlike these four oligosaccharides, Chito-oligosaccharides (COS) changed the secondary and tertiary structure of β-Gal, thus decreasing its activity retention rate.					
31260766	0	24	theme	high	28:31	arg1	Oligosaccharides					0:15	Oligosaccharides	0:15	Oligosaccharides	0:15	Oligosaccharides act as the high efficiency stabilizer for β-galactosidase under heat treatment.					
31260766	0	24	theme	high	28:31	arg1	stabilizer					44:53	the high efficiency stabilizer	24:53	the high efficiency stabilizer for β-galactosidase under heat treatment	24:94	Oligosaccharides act as the high efficiency stabilizer for β-galactosidase under heat treatment.					
31260766	4	25	theme	assays	543:548	arg1	results					526:532	The results	522:532	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR)	522:643	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	4	26	dep	Fourier	598:604	arg1	transform					606:614	transform	606:614	transform infrared spectroscopy (FTIR)	606:643	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	7	27	theme	lactase	1350:1356	arg1	application					1328:1338	application	1328:1338	application of liquid lactase to food industry	1328:1373	This study provides an insight into the mechanism by which sugar stabilizes protein under heat stress and offers guidance for application of liquid lactase to food industry.					
31260766	3	28	theme	activity	472:479	arg1	retention					481:489	the activity retention	468:489	the activity retention of β-Gal	468:498	Four kinds of oligosaccharides including Isomalto-oligosaccharides (IMO), Xylo-oligosaccharides (XOS), Konjac-oligosaccharides (KOS), and Mycose significantly increased the activity retention of β-Gal under heat treatment.					
31260766	6	29	theme	activity	998:1005	arg1	rate					1017:1020	the activity retention rate	994:1020	the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v)	994:1086	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	6	30	theme	%	1198:1198	arg1	rate					1188:1191	the activity retention rate	1165:1191	the activity retention rate of 20%	1165:1198	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	3	31	theme	heat	506:509	arg1	treatment					511:519	heat treatment	506:519	heat treatment	506:519	Four kinds of oligosaccharides including Isomalto-oligosaccharides (IMO), Xylo-oligosaccharides (XOS), Konjac-oligosaccharides (KOS), and Mycose significantly increased the activity retention of β-Gal under heat treatment.					
31260766	4	32	theme	circular	560:567	arg1	dichroism					569:577	circular dichroism	560:577	circular dichroism	560:577	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	3	33	theme	oligosaccharides	313:328	arg1	kinds					304:308	Four kinds	299:308	Four kinds of oligosaccharides including Isomalto-oligosaccharides (IMO), Xylo-oligosaccharides (XOS), Konjac-oligosaccharides (KOS), and Mycose	299:442	Four kinds of oligosaccharides including Isomalto-oligosaccharides (IMO), Xylo-oligosaccharides (XOS), Konjac-oligosaccharides (KOS), and Mycose significantly increased the activity retention of β-Gal under heat treatment.					
31260766	4	34	theme	β-Gal	741:745	arg1	structure					728:736	the secondary and tertiary structure	701:736	the secondary and tertiary structure of β-Gal	701:745	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	6	35	theme	activity	1169:1176	arg1	rate					1188:1191	the activity retention rate	1165:1191	the activity retention rate of 20%	1165:1198	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	1	36	theme	dietary	124:130	arg1	supplement					132:141	dietary supplement	124:141	dietary supplement	124:141	β-Galactosidase (β-Gal) as dietary supplement has the ability to alleviate symptoms of lactose intolerance.					
31260766	6	37	dep	β-Gal	1158:1162	arg1	rate					1188:1191	the activity retention rate	1165:1191	the activity retention rate of 20%	1165:1198	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	6	38	theme	%	1076:1076	arg1	IMO					1061:1063	30% IMO	1057:1063	30% IMO	1057:1063	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	6	38	theme	%	1076:1076	arg1	w/v					1083:1085	w/v	1083:1085	w/v	1083:1085	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	6	38	theme	%	1076:1076	arg1	XOS					1078:1080	40% XOS	1074:1080	40% XOS	1074:1080	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	4	39	theme	thermal	753:759	arg1	conditions					761:770	thermal conditions	753:770	thermal conditions	753:770	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	3	40	theme	β-Gal	494:498	arg1	retention					481:489	the activity retention	468:489	the activity retention of β-Gal	468:498	Four kinds of oligosaccharides including Isomalto-oligosaccharides (IMO), Xylo-oligosaccharides (XOS), Konjac-oligosaccharides (KOS), and Mycose significantly increased the activity retention of β-Gal under heat treatment.					
31260766	6	41	theme	retention	1178:1186	arg1	rate					1188:1191	the activity retention rate	1165:1191	the activity retention rate of 20%	1165:1198	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	5	42	theme	secondary	884:892	arg1	structure					907:915	the secondary and tertiary structure	880:915	the secondary and tertiary structure of β-Gal	880:924	Unlike these four oligosaccharides, Chito-oligosaccharides (COS) changed the secondary and tertiary structure of β-Gal, thus decreasing its activity retention rate.					
31260766	4	43	theme	tertiary	719:726	arg1	structure					728:736	the secondary and tertiary structure	701:736	the secondary and tertiary structure of β-Gal	701:745	The results of three assays including circular dichroism, fluorescence, and Fourier transform infrared spectroscopy (FTIR) illustrated that these oligosaccharides could stabilize the secondary and tertiary structure of β-Gal under thermal conditions through hydrogen bond interaction.					
31260766	6	44	theme	%	1059:1059	arg1	w/v					1066:1068	w/v	1066:1068	w/v	1066:1068	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	6	44	theme	%	1059:1059	arg1	IMO					1061:1063	30% IMO	1057:1063	30% IMO	1057:1063	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	6	44	theme	%	1059:1059	arg1	XOS					1078:1080	40% XOS	1074:1080	40% XOS	1074:1080	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	6	45	theme	heat	978:981	arg1	treatment					983:991	heat treatment	978:991	heat treatment	978:991	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
31260766	2	46	theme	oligosaccharides	244:259	arg1	ability					233:239	the ability	229:239	the ability of oligosaccharides to protect β-Gal against heat stress	229:296	This study investigated the ability of oligosaccharides to protect β-Gal against heat stress.					
31260766	6	47	theme	30	1057:1058	arg1	%					1059:1059	%	1059:1059	%	1059:1059	Under heat treatment, the activity retention rate of β-Gal with optimal composition (30% IMO, w/v and 40% XOS, w/v) reached 82.1%, which was significantly higher than that of the native β-Gal (the activity retention rate of 20%).					
29393491	8	0	theme	resistance	1246:1255	arg1	development					1219:1229	the development	1215:1229	the development of oxaliplatin resistance in colon cancer cells	1215:1277	These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.					
29393491	9	1	theme	drug	1394:1397	arg1	resistance					1399:1408	drug resistance	1394:1408	drug resistance in colon cancer	1394:1424	These findings may be valuable for overcoming drug resistance in colon cancer.					
29393491	3	2	theme	oxaliplatin-resistant	544:564	arg1	cells					573:577	oxaliplatin-resistant SW620R cells	544:577	oxaliplatin-resistant SW620R cells	544:577	Using the lectin microarray for glycan composition and FITC-lectin binding for glycan profiling, we found that polylactosamine-type N-glycans were significantly increased in oxaliplatin-resistant SW620R cells.					
29393491	0	3	theme	cancer	87:92	arg1	cells					94:98	colon cancer cells	81:98	colon cancer cells	81:98	β3GnT8 regulates oxaliplatin resistance by altering integrin β1 glycosylation in colon cancer cells.					
29393491	8	4	theme	glycosylation	1318:1330	arg1	alteration					1300:1309	the alteration	1296:1309	the alteration of the glycosylation of integrin β1	1296:1345	These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.					
29393491	3	5	theme	SW620R	566:571	arg1	cells					573:577	oxaliplatin-resistant SW620R cells	544:577	oxaliplatin-resistant SW620R cells	544:577	Using the lectin microarray for glycan composition and FITC-lectin binding for glycan profiling, we found that polylactosamine-type N-glycans were significantly increased in oxaliplatin-resistant SW620R cells.					
29393491	6	6	theme	SW620	940:944	arg1	cells					946:950	SW620 cells	940:950	SW620 cells	940:950	Conversely, the engineered overexpression of β3GnT8 in SW620 cells enhanced resistance to oxaliplatin.					
29393491	8	7	theme	colon	1260:1264	arg1	cells					1273:1277	colon cancer cells	1260:1277	colon cancer cells	1260:1277	These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.					
29393491	7	8	theme	polylactosamine	1057:1071	arg1	chains					1073:1078	polylactosamine chains	1057:1078	polylactosamine chains on integrin β1	1057:1093	Further data revealed that manipulation of β3GnT8 was able to modify polylactosamine chains on integrin β1 and to regulate the integrin β1 downstream signaling pathway.					
29393491	2	9	from	resistance	330:339	arg1	cells					363:367	human colon cancer cells	344:367	human colon cancer cells	344:367	The present study was aimed to investigate the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells.					
29393491	3	10	theme	lectin	380:385	arg1	microarray					387:396	the lectin microarray	376:396	the lectin microarray for glycan composition and FITC-lectin binding for glycan profiling	376:464	Using the lectin microarray for glycan composition and FITC-lectin binding for glycan profiling, we found that polylactosamine-type N-glycans were significantly increased in oxaliplatin-resistant SW620R cells.					
29393491	9	11	theme	colon	1413:1417	arg1	cancer					1419:1424	colon cancer	1413:1424	colon cancer	1413:1424	These findings may be valuable for overcoming drug resistance in colon cancer.					
29393491	5	12	theme	β3GnT8	807:812	arg1	silencing					794:802	the silencing	790:802	the silencing of β3GnT8 in SW620R cells	790:828	Using an RNA interference strategy, we revealed that the silencing of β3GnT8 in SW620R cells resulted in increased chemosensitivity to oxaliplatin.					
29393491	2	13	theme	glycogene	284:292	arg1	alterations					294:304	the glycan and glycogene alterations	269:304	the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells	269:367	The present study was aimed to investigate the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells.					
29393491	7	14	from	chains	1073:1078	arg1	integrin β1					1083:1093	integrin β1	1083:1093	integrin β1	1083:1093	Further data revealed that manipulation of β3GnT8 was able to modify polylactosamine chains on integrin β1 and to regulate the integrin β1 downstream signaling pathway.					
29393491	3	15	theme	FITC-lectin	425:435	arg1	binding					437:443	FITC-lectin binding	425:443	FITC-lectin binding	425:443	Using the lectin microarray for glycan composition and FITC-lectin binding for glycan profiling, we found that polylactosamine-type N-glycans were significantly increased in oxaliplatin-resistant SW620R cells.					
29393491	6	16	from	overexpression	912:925	arg1	cells					946:950	SW620 cells	940:950	SW620 cells	940:950	Conversely, the engineered overexpression of β3GnT8 in SW620 cells enhanced resistance to oxaliplatin.					
29393491	7	17	theme	β3GnT8	1031:1036	arg1	manipulation					1015:1026	manipulation	1015:1026	manipulation of β3GnT8	1015:1036	Further data revealed that manipulation of β3GnT8 was able to modify polylactosamine chains on integrin β1 and to regulate the integrin β1 downstream signaling pathway.					
29393491	8	18	theme	integrin β1	1335:1345	arg1	glycosylation					1318:1330	the glycosylation	1314:1330	the glycosylation of integrin β1	1314:1345	These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.					
29393491	3	19	theme	glycan	402:407	arg1	composition					409:419	glycan composition	402:419	glycan composition	402:419	Using the lectin microarray for glycan composition and FITC-lectin binding for glycan profiling, we found that polylactosamine-type N-glycans were significantly increased in oxaliplatin-resistant SW620R cells.					
29393491	8	20	theme	oxaliplatin	1234:1244	arg1	resistance					1246:1255	oxaliplatin resistance	1234:1255	oxaliplatin resistance	1234:1255	These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.					
29393491	5	21	theme	SW620R	817:822	arg1	cells					824:828	SW620R cells	817:828	SW620R cells	817:828	Using an RNA interference strategy, we revealed that the silencing of β3GnT8 in SW620R cells resulted in increased chemosensitivity to oxaliplatin.					
29393491	8	22	theme	key	1203:1205	arg1	role					1207:1210	a key role	1201:1210	a key role	1201:1210	These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.					
29393491	3	23	theme	glycan	449:454	arg1	profiling					456:464	glycan profiling	449:464	glycan profiling	449:464	Using the lectin microarray for glycan composition and FITC-lectin binding for glycan profiling, we found that polylactosamine-type N-glycans were significantly increased in oxaliplatin-resistant SW620R cells.					
29393491	8	24	gly	glycosylation	1318:1330	arg1	integrin β1					1335:1345	integrin β1	1335:1345	integrin β1	1335:1345	These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.					
29393491	2	25	theme	oxaliplatin	318:328	arg1	resistance					330:339	oxaliplatin resistance	318:339	oxaliplatin resistance in human colon cancer cells	318:367	The present study was aimed to investigate the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells.					
29393491	3	26	theme	polylactosamine-type	481:500	arg1	N-glycans					502:510	polylactosamine-type N-glycans	481:510	polylactosamine-type N-glycans	481:510	Using the lectin microarray for glycan composition and FITC-lectin binding for glycan profiling, we found that polylactosamine-type N-glycans were significantly increased in oxaliplatin-resistant SW620R cells.					
29393491	0	27	theme	oxaliplatin	17:27	arg1	resistance					29:38	oxaliplatin resistance	17:38	oxaliplatin resistance	17:38	β3GnT8 regulates oxaliplatin resistance by altering integrin β1 glycosylation in colon cancer cells.					
29393491	1	28	theme	drug	122:125	arg1	resistance					127:136	drug resistance	122:136	drug resistance	122:136	Correlations between drug resistance and glycosylation changes have been analyzed intensively in the field of tumor biology.					
29393491	9	29	from	resistance	1399:1408	arg1	cancer					1419:1424	colon cancer	1413:1424	colon cancer	1413:1424	These findings may be valuable for overcoming drug resistance in colon cancer.					
29393491	1	30	theme	biology	217:223	arg1	field					202:206	the field	198:206	the field of tumor biology	198:223	Correlations between drug resistance and glycosylation changes have been analyzed intensively in the field of tumor biology.					
29393491	5	31	theme	increased	842:850	arg1	chemosensitivity					852:867	increased chemosensitivity	842:867	increased chemosensitivity to oxaliplatin	842:882	Using an RNA interference strategy, we revealed that the silencing of β3GnT8 in SW620R cells resulted in increased chemosensitivity to oxaliplatin.					
29393491	4	32	theme	glycogenes	622:631	arg1	quantification					604:617	quantification	604:617	quantification of glycogenes	604:631	Using real-time PCR for quantification of glycogenes, we targeted β-1,3-N-acetylglucosaminyltransferase 8 (β3GnT8), which was overexpressed in SW620R cells.					
29393491	4	33	theme	SW620R	723:728	arg1	cells					730:734	SW620R cells	723:734	SW620R cells	723:734	Using real-time PCR for quantification of glycogenes, we targeted β-1,3-N-acetylglucosaminyltransferase 8 (β3GnT8), which was overexpressed in SW620R cells.					
29393491	4	34	theme	real-time	586:594	arg1	PCR					596:598	real-time PCR	586:598	real-time PCR for quantification of glycogenes	586:631	Using real-time PCR for quantification of glycogenes, we targeted β-1,3-N-acetylglucosaminyltransferase 8 (β3GnT8), which was overexpressed in SW620R cells.					
29393491	5	35	from	silencing	794:802	arg1	cells					824:828	SW620R cells	817:828	SW620R cells	817:828	Using an RNA interference strategy, we revealed that the silencing of β3GnT8 in SW620R cells resulted in increased chemosensitivity to oxaliplatin.					
29393491	8	36	theme	cancer	1266:1271	arg1	cells					1273:1277	colon cancer cells	1260:1277	colon cancer cells	1260:1277	These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.					
29393491	5	37	theme	RNA	746:748	arg1	interference					750:761	RNA interference	746:761	an RNA interference strategy	743:770	Using an RNA interference strategy, we revealed that the silencing of β3GnT8 in SW620R cells resulted in increased chemosensitivity to oxaliplatin.					
29393491	8	38	from	development	1219:1229	arg1	cells					1273:1277	colon cancer cells	1260:1277	colon cancer cells	1260:1277	These results revealed that β3GnT8 may play a key role in the development of oxaliplatin resistance in colon cancer cells possibly through the alteration of the glycosylation of integrin β1.					
29393491	7	39	theme	integrin β1	1115:1125	arg1	pathway					1148:1154	the integrin β1 downstream signaling pathway	1111:1154	the integrin β1 downstream signaling pathway	1111:1154	Further data revealed that manipulation of β3GnT8 was able to modify polylactosamine chains on integrin β1 and to regulate the integrin β1 downstream signaling pathway.					
29393491	0	40	theme	integrin β1	52:62	arg1	glycosylation					64:76	integrin β1 glycosylation	52:76	integrin β1 glycosylation	52:76	β3GnT8 regulates oxaliplatin resistance by altering integrin β1 glycosylation in colon cancer cells.					
29393491	5	41	theme	interference	750:761	arg1	strategy					763:770	an RNA interference strategy	743:770	an RNA interference strategy	743:770	Using an RNA interference strategy, we revealed that the silencing of β3GnT8 in SW620R cells resulted in increased chemosensitivity to oxaliplatin.					
29393491	6	42	theme	β3GnT8	930:935	arg1	overexpression					912:925	the engineered overexpression	897:925	the engineered overexpression of β3GnT8 in SW620 cells	897:950	Conversely, the engineered overexpression of β3GnT8 in SW620 cells enhanced resistance to oxaliplatin.					
29393491	2	43	theme	present	230:236	arg1	study					238:242	The present study	226:242	The present study	226:242	The present study was aimed to investigate the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells.					
29393491	2	44	theme	cancer	356:361	arg1	cells					363:367	human colon cancer cells	344:367	human colon cancer cells	344:367	The present study was aimed to investigate the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells.					
29393491	7	45	dep	able	1042:1045	arg1	regulate					1102:1109	regulate	1102:1109	to regulate the integrin β1 downstream signaling pathway	1099:1154	Further data revealed that manipulation of β3GnT8 was able to modify polylactosamine chains on integrin β1 and to regulate the integrin β1 downstream signaling pathway.					
29393491	7	45	dep	able	1042:1045	arg1	modify					1050:1055	modify	1050:1055	to modify polylactosamine chains on integrin β1	1047:1093	Further data revealed that manipulation of β3GnT8 was able to modify polylactosamine chains on integrin β1 and to regulate the integrin β1 downstream signaling pathway.					
29393491	7	46	theme	downstream	1127:1136	arg1	pathway					1148:1154	the integrin β1 downstream signaling pathway	1111:1154	the integrin β1 downstream signaling pathway	1111:1154	Further data revealed that manipulation of β3GnT8 was able to modify polylactosamine chains on integrin β1 and to regulate the integrin β1 downstream signaling pathway.					
29393491	2	47	theme	glycan	273:278	arg1	alterations					294:304	the glycan and glycogene alterations	269:304	the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells	269:367	The present study was aimed to investigate the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells.					
29393491	1	48	theme	glycosylation	142:154	arg1	changes					156:162	glycosylation changes	142:162	glycosylation changes	142:162	Correlations between drug resistance and glycosylation changes have been analyzed intensively in the field of tumor biology.					
29393491	7	49	theme	signaling	1138:1146	arg1	pathway					1148:1154	the integrin β1 downstream signaling pathway	1111:1154	the integrin β1 downstream signaling pathway	1111:1154	Further data revealed that manipulation of β3GnT8 was able to modify polylactosamine chains on integrin β1 and to regulate the integrin β1 downstream signaling pathway.					
29393491	2	50	theme	human	344:348	arg1	cells					363:367	human colon cancer cells	344:367	human colon cancer cells	344:367	The present study was aimed to investigate the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells.					
29393491	7	51	theme	Further	988:994	arg1	data					996:999	Further data	988:999	Further data	988:999	Further data revealed that manipulation of β3GnT8 was able to modify polylactosamine chains on integrin β1 and to regulate the integrin β1 downstream signaling pathway.					
29393491	2	52	theme	colon	350:354	arg1	cells					363:367	human colon cancer cells	344:367	human colon cancer cells	344:367	The present study was aimed to investigate the glycan and glycogene alterations involved in oxaliplatin resistance in human colon cancer cells.					
29393491	0	53	theme	colon	81:85	arg1	cells					94:98	colon cancer cells	81:98	colon cancer cells	81:98	β3GnT8 regulates oxaliplatin resistance by altering integrin β1 glycosylation in colon cancer cells.					
29393491	6	54	theme	engineered	901:910	arg1	overexpression					912:925	the engineered overexpression	897:925	the engineered overexpression of β3GnT8 in SW620 cells	897:950	Conversely, the engineered overexpression of β3GnT8 in SW620 cells enhanced resistance to oxaliplatin.					
29393491	1	55	theme	tumor	211:215	arg1	biology					217:223	tumor biology	211:223	tumor biology	211:223	Correlations between drug resistance and glycosylation changes have been analyzed intensively in the field of tumor biology.					
29393491	0	56	gly	glycosylation	64:76	arg1	cells					94:98	colon cancer cells	81:98	colon cancer cells	81:98	β3GnT8 regulates oxaliplatin resistance by altering integrin β1 glycosylation in colon cancer cells.					
31740752	3	0	from	genes	580:584	arg1	enriched					544:551	enriched	544:551	enriched	544:551	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	7	1	theme	infant	1515:1520	arg1	ecosystem					1522:1530	the infant ecosystem	1511:1530	the infant ecosystem	1511:1530	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	8	2	theme	future	1701:1706	arg1	development					1708:1718	future development	1701:1718	future development of new diet and microbiota-based therapies to promote infant health	1701:1786	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	8	3	theme	infant	1774:1779	arg1	health					1781:1786	infant health	1774:1786	infant health	1774:1786	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	4	4	theme	FL	895:896	arg1	HMOs					907:910	2'FL and LNnT HMOs	893:910	2'FL and LNnT HMOs between strains	893:926	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	6	5	theme	modest	1211:1216	arg1	growth					1218:1223	modest growth	1211:1223	modest growth of non-HMO users on spend media from HMO metabolism	1211:1275	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	4	6	theme	human	727:731	arg1	HMOs					756:759	HMOs	756:759	HMOs	756:759	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	4	6	theme	human	727:731	arg1	oligosaccharides					738:753	human milk oligosaccharides	727:753	human milk oligosaccharides (HMOs)	727:760	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	3	7	from	enriched	544:551	arg1	genes					580:584	carbohydrate metabolism genes	556:584	carbohydrate metabolism genes	556:584	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	4	8	theme	LNnT	902:905	arg1	HMOs					907:910	2'FL and LNnT HMOs	893:910	2'FL and LNnT HMOs between strains	893:926	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	7	9	theme	strains	1461:1467	arg1	role					1479:1482	their role	1473:1482	their role as 'foundation' species in the infant ecosystem	1473:1530	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	7	9	theme	strains	1461:1467	arg1	nature					1435:1440	the cooperative nature	1419:1440	the cooperative nature of bifidobacterial strains	1419:1467	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	4	10	theme	oligosaccharides	738:753	arg1	digestion					714:722	digestion	714:722	digestion of human milk oligosaccharides (HMOs)	714:760	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	2	11	theme	infant	316:321	arg1	diet					323:326	infant diet	316:326	infant diet	316:326	Here, we sought to explore how infant diet drives distinct bifidobacterial community composition and dynamics within individual infant ecosystems.					
31740752	5	12	theme	HMO	975:977	arg1	degraders					979:987	HMO degraders	975:987	HMO degraders	975:987	Cross-feeding experiments were performed with HMO degraders and non-HMO users (using spent or 'conditioned' media and direct co-culture).					
31740752	7	13	theme	cooperative	1423:1433	arg1	nature					1435:1440	the cooperative nature	1419:1440	the cooperative nature of bifidobacterial strains	1419:1467	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	0	14	theme	milk	26:29	arg1	oligosaccharides					31:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem.					
31740752	8	15	dep	diversity	1619:1627	arg1	the					1615:1617	the	1615:1617	the	1615:1617	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	1	16	theme	important	146:154	arg1	role					156:159	an important role	143:159	an important role	143:159	Diet-microbe interactions play an important role in modulating the early-life microbiota, with Bifidobacterium strains and species dominating the gut of breast-fed infants.					
31740752	6	17	theme	users	1236:1240	arg1	growth					1218:1223	modest growth	1211:1223	modest growth of non-HMO users on spend media from HMO metabolism	1211:1275	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	4	18	from	differences	855:865	arg1	ability					874:880	the ability to utilise 2'FL and LNnT HMOs between strains	870:926	the ability to utilise 2'FL and LNnT HMOs between strains	870:926	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	0	19	theme	single	94:99	arg1	ecosystem					101:109	a single ecosystem	92:109	a single ecosystem	92:109	Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem.					
31740752	4	20	theme	single	829:834	arg1	infants					836:842	single infants	829:842	single infants	829:842	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	8	21	theme	bifidobacterial	1561:1575	arg1	behaviour					1587:1595	The intra- and inter-infant bifidobacterial community behaviour	1533:1595	The intra- and inter-infant bifidobacterial community behaviour	1533:1595	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	5	22	theme	conditioned	1024:1034	arg1	media					1037:1041	spent or 'conditioned' media	1014:1041	spent or 'conditioned' media	1014:1041	Cross-feeding experiments were performed with HMO degraders and non-HMO users (using spent or 'conditioned' media and direct co-culture).					
31740752	7	23	from	diet	1399:1402	arg1	consumption					1378:1388	nutrient consumption	1369:1388	nutrient consumption from the diet	1369:1402	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	3	24	theme	Genomic	432:438	arg1	characterisation					440:455	Genomic characterisation	432:455	Genomic characterisation of 19 strains isolated from breast-fed infants	432:502	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	6	25	from	growth	1218:1223	arg1	media					1251:1255	spend media	1245:1255	spend media from HMO metabolism	1245:1275	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	0	26	theme	Breast	0:5	arg1	oligosaccharides					31:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem.					
31740752	1	27	theme	Diet-microbe	112:123	arg1	interactions					125:136	Diet-microbe interactions	112:136	Diet-microbe interactions	112:136	Diet-microbe interactions play an important role in modulating the early-life microbiota, with Bifidobacterium strains and species dominating the gut of breast-fed infants.					
31740752	3	28	theme	genomic	523:529	arg1	architecture					531:542	a diverse genomic architecture	513:542	a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants	513:671	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	0	29	theme	human	20:24	arg1	oligosaccharides					31:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem.					
31740752	3	30	theme	strains	463:469	arg1	characterisation					440:455	Genomic characterisation	432:455	Genomic characterisation of 19 strains isolated from breast-fed infants	432:502	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	6	31	theme	1H-NMR	1075:1080	arg1	analysis					1082:1089	Further 1H-NMR analysis	1067:1089	Further 1H-NMR analysis	1067:1089	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	4	32	theme	2	893:893	arg1	FL					895:896	FL	895:896	FL	895:896	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	3	33	theme	carbohydrate	556:567	arg1	genes					580:584	carbohydrate metabolism genes	556:584	carbohydrate metabolism genes	556:584	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	0	34	theme	Bifidobacterium	56:70	arg1	interactions					72:83	Bifidobacterium interactions	56:83	Bifidobacterium interactions	56:83	Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem.					
31740752	2	35	theme	community	360:368	arg1	composition					370:380	distinct bifidobacterial community composition	335:380	distinct bifidobacterial community composition	335:380	Here, we sought to explore how infant diet drives distinct bifidobacterial community composition and dynamics within individual infant ecosystems.					
31740752	7	36	theme	HMO	1309:1311	arg1	metabolism					1313:1322	HMO metabolism	1309:1322	HMO metabolism	1309:1322	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	2	37	theme	infant	413:418	arg1	ecosystems					420:429	individual infant ecosystems	402:429	individual infant ecosystems	402:429	Here, we sought to explore how infant diet drives distinct bifidobacterial community composition and dynamics within individual infant ecosystems.					
31740752	8	38	theme	new	1723:1725	arg1	diet					1727:1730	new diet	1723:1730	new diet	1723:1730	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	1	39	theme	breast-fed	265:274	arg1	infants					276:282	breast-fed infants	265:282	breast-fed infants	265:282	Diet-microbe interactions play an important role in modulating the early-life microbiota, with Bifidobacterium strains and species dominating the gut of breast-fed infants.					
31740752	5	40	theme	direct	1047:1052	arg1	co-culture					1054:1063	direct co-culture	1047:1063	direct co-culture	1047:1063	Cross-feeding experiments were performed with HMO degraders and non-HMO users (using spent or 'conditioned' media and direct co-culture).					
31740752	8	41	from	diversity	1619:1627	arg1	life					1671:1674	early life	1665:1674	early life	1665:1674	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	8	42	theme	therapies	1753:1761	arg1	development					1708:1718	future development	1701:1718	future development of new diet and microbiota-based therapies to promote infant health	1701:1786	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	0	43	link	milk-derived	7:18	arg1	oligosaccharides					31:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem.					
31740752	7	44	theme	foundation	1488:1497	arg1	species					1500:1506	'foundation' species	1487:1506	'foundation' species	1487:1506	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	8	45	from	dominance	1633:1641	arg1	life					1671:1674	early life	1665:1674	early life	1665:1674	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	2	46	theme	distinct	335:342	arg1	composition					370:380	distinct bifidobacterial community composition	335:380	distinct bifidobacterial community composition	335:380	Here, we sought to explore how infant diet drives distinct bifidobacterial community composition and dynamics within individual infant ecosystems.					
31740752	1	47	theme	Bifidobacterium	207:221	arg1	strains					223:229	Bifidobacterium strains	207:229	Bifidobacterium strains	207:229	Diet-microbe interactions play an important role in modulating the early-life microbiota, with Bifidobacterium strains and species dominating the gut of breast-fed infants.					
31740752	4	48	theme	milk	733:736	arg1	HMOs					756:759	HMOs	756:759	HMOs	756:759	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	4	48	theme	milk	733:736	arg1	oligosaccharides					738:753	human milk oligosaccharides	727:753	human milk oligosaccharides (HMOs)	727:760	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	3	49	attach	isolated	471:478	arg1	infants					496:502	breast-fed infants	485:502	breast-fed infants	485:502	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	3	49	attach	isolated	471:478	arg2	strains					463:469	19 strains	460:469	19 strains isolated from breast-fed infants	460:502	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	7	50	theme	bifidobacterial	1445:1459	arg1	strains					1461:1467	bifidobacterial strains	1445:1467	bifidobacterial strains	1445:1467	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	4	51	theme	clusters	691:698	arg1	Presence					674:681	Presence	674:681	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs)	674:760	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	6	52	theme	HMO	1262:1264	arg1	metabolism					1266:1275	HMO metabolism	1262:1275	HMO metabolism	1262:1275	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	6	53	theme	Further	1067:1073	arg1	analysis					1082:1089	Further 1H-NMR analysis	1067:1089	Further 1H-NMR analysis	1067:1089	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	6	54	from	metabolism	1266:1275	arg1	media					1251:1255	spend media	1245:1255	spend media from HMO metabolism	1245:1275	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	3	55	theme	enriched	544:551	arg1	architecture					531:542	a diverse genomic architecture	513:542	a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants	513:671	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	5	56	theme	Cross-feeding	929:941	arg1	experiments					943:953	Cross-feeding experiments	929:953	Cross-feeding experiments	929:953	Cross-feeding experiments were performed with HMO degraders and non-HMO users (using spent or 'conditioned' media and direct co-culture).					
31740752	8	57	theme	intra-	1537:1542	arg1	behaviour					1587:1595	The intra- and inter-infant bifidobacterial community behaviour	1533:1595	The intra- and inter-infant bifidobacterial community behaviour	1533:1595	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	6	58	theme	non-HMO	1228:1234	arg1	users					1236:1240	non-HMO users	1228:1240	non-HMO users	1228:1240	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	6	59	dep	media	1251:1255	arg1	spend					1245:1249	spend	1245:1249	spend	1245:1249	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	1	60	theme	early-life	179:188	arg1	microbiota					190:199	the early-life microbiota	175:199	the early-life microbiota	175:199	Diet-microbe interactions play an important role in modulating the early-life microbiota, with Bifidobacterium strains and species dominating the gut of breast-fed infants.					
31740752	7	61	from	role	1479:1482	arg1	ecosystem					1522:1530	the infant ecosystem	1511:1530	the infant ecosystem	1511:1530	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	8	62	theme	inter-infant	1548:1559	arg1	behaviour					1587:1595	The intra- and inter-infant bifidobacterial community behaviour	1533:1595	The intra- and inter-infant bifidobacterial community behaviour	1533:1595	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	5	63	dep	users	1001:1005	arg1	using					1008:1012	using	1008:1012	using spent or 'conditioned' media and direct co-culture	1008:1063	Cross-feeding experiments were performed with HMO degraders and non-HMO users (using spent or 'conditioned' media and direct co-culture).					
31740752	5	64	theme	spent	1014:1018	arg1	media					1037:1041	spent or 'conditioned' media	1014:1041	spent or 'conditioned' media	1014:1041	Cross-feeding experiments were performed with HMO degraders and non-HMO users (using spent or 'conditioned' media and direct co-culture).					
31740752	6	65	theme	metabolism	1180:1189	arg1	by-products					1161:1171	key by-products	1157:1171	key by-products of HMO metabolism	1157:1189	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	8	66	theme	community	1577:1585	arg1	behaviour					1587:1595	The intra- and inter-infant bifidobacterial community behaviour	1533:1595	The intra- and inter-infant bifidobacterial community behaviour	1533:1595	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	6	67	theme	key	1157:1159	arg1	by-products					1161:1171	key by-products	1157:1171	key by-products of HMO metabolism	1157:1189	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	7	68	theme	nutrient	1369:1376	arg1	consumption					1378:1388	nutrient consumption	1369:1388	nutrient consumption from the diet	1369:1402	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	0	69	theme	milk-derived	7:18	arg1	oligosaccharides					31:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides	0:46	Breast milk-derived human milk oligosaccharides promote Bifidobacterium interactions within a single ecosystem.					
31740752	5	70	theme	non-HMO	993:999	arg1	users					1001:1005	non-HMO users	993:1005	non-HMO users (using spent or 'conditioned' media and direct co-culture)	993:1064	Cross-feeding experiments were performed with HMO degraders and non-HMO users (using spent or 'conditioned' media and direct co-culture).					
31740752	4	71	theme	growth	791:796	arg1	studies					798:804	growth studies	791:804	growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains	791:926	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	3	72	theme	breast-fed	485:494	arg1	infants					496:502	breast-fed infants	485:502	breast-fed infants	485:502	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	4	73	dep	FL	895:896	arg1	'					894:894	'	894:894	'	894:894	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	7	74	from	nature	1435:1440	arg1	ecosystem					1522:1530	the infant ecosystem	1511:1530	the infant ecosystem	1511:1530	These experiments indicate how HMO metabolism permits the sharing of resources to maximise nutrient consumption from the diet and highlights the cooperative nature of bifidobacterial strains and their role as 'foundation' species in the infant ecosystem.					
31740752	3	75	theme	diverse	515:521	arg1	architecture					531:542	a diverse genomic architecture	513:542	a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants	513:671	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	6	76	theme	HMO	1176:1178	arg1	metabolism					1180:1189	HMO metabolism	1176:1189	HMO metabolism	1176:1189	Further 1H-NMR analysis identified fucose, galactose, acetate, and N-acetylglucosamine as key by-products of HMO metabolism; as demonstrated by modest growth of non-HMO users on spend media from HMO metabolism.					
31740752	8	77	theme	Bifidobacterium	1646:1660	arg1	dominance					1633:1641	dominance	1633:1641	dominance	1633:1641	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	8	77	theme	Bifidobacterium	1646:1660	arg1	diversity					1619:1627	diversity	1619:1627	diversity	1619:1627	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	3	78	theme	metabolism	569:578	arg1	genes					580:584	carbohydrate metabolism genes	556:584	carbohydrate metabolism genes	556:584	Genomic characterisation of 19 strains isolated from breast-fed infants revealed a diverse genomic architecture enriched in carbohydrate metabolism genes, which was distinct to each strain, but collectively formed a pangenome across infants.					
31740752	8	79	theme	early	1665:1669	arg1	life					1671:1674	early life	1665:1674	early life	1665:1674	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	2	80	theme	bifidobacterial	344:358	arg1	composition					370:380	distinct bifidobacterial community composition	335:380	distinct bifidobacterial community composition	335:380	Here, we sought to explore how infant diet drives distinct bifidobacterial community composition and dynamics within individual infant ecosystems.					
31740752	8	81	theme	diet	1727:1730	arg1	development					1708:1718	future development	1701:1718	future development of new diet and microbiota-based therapies to promote infant health	1701:1786	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	4	82	theme	gene	686:689	arg1	clusters					691:698	gene clusters	686:698	gene clusters implicated in digestion of human milk oligosaccharides (HMOs)	686:760	Presence of gene clusters implicated in digestion of human milk oligosaccharides (HMOs) varied between species, with growth studies indicating that within single infants there were differences in the ability to utilise 2'FL and LNnT HMOs between strains.					
31740752	2	83	theme	individual	402:411	arg1	ecosystems					420:429	individual infant ecosystems	402:429	individual infant ecosystems	402:429	Here, we sought to explore how infant diet drives distinct bifidobacterial community composition and dynamics within individual infant ecosystems.					
31740752	8	84	theme	microbiota-based	1736:1751	arg1	therapies					1753:1761	microbiota-based therapies	1736:1761	microbiota-based therapies	1736:1761	The intra- and inter-infant bifidobacterial community behaviour may contribute to the diversity and dominance of Bifidobacterium in early life and suggests avenues for future development of new diet and microbiota-based therapies to promote infant health.					
31740752	1	85	theme	infants	276:282	arg1	gut					258:260	the gut	254:260	the gut of breast-fed infants	254:282	Diet-microbe interactions play an important role in modulating the early-life microbiota, with Bifidobacterium strains and species dominating the gut of breast-fed infants.					
29807078	3	0	theme	different	563:571	arg1	sizes					573:577	different sizes	563:577	different sizes	563:577	The morphology of the alginate gel beads are changed after functionalization and are characterized by aggregates of different sizes and irregular shapes.					
29807078	3	1	theme	sizes	573:577	arg1	aggregates					549:558	aggregates	549:558	aggregates of different sizes and irregular shapes	549:598	The morphology of the alginate gel beads are changed after functionalization and are characterized by aggregates of different sizes and irregular shapes.					
29807078	4	2	theme	Blue	641:644	arg1	Methylene					631:639	Methylene Blue	631:644	Methylene Blue	631:644	The adsorption is checked for Methylene Blue and Acid Blue 25 in batch mode with reference to the change of pH, time, temperature, and adsorbate concentration.					
29807078	0	3	theme	composite	124:132	arg1	beads					138:142	ethoxy-silica] composite gel beads	109:142	ethoxy-silica] composite gel beads	109:142	Improved removal of dyes by [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads.					
29807078	8	4	theme	kinetic	1007:1013	arg1	data					1015:1018	The kinetic data	1003:1018	The kinetic data	1003:1018	The kinetic data complies well with the second order.					
29807078	7	5	theme	possible	913:920	arg1	formation					930:938	A possible complex formation	911:938	A possible complex formation of composite gel beads and the studied adsorbates	911:988	A possible complex formation of composite gel beads and the studied adsorbates is proposed.					
29807078	4	6	theme	Blue	655:658	arg1	Acid					650:653	Acid Blue 25	650:661	Acid Blue 25	650:661	The adsorption is checked for Methylene Blue and Acid Blue 25 in batch mode with reference to the change of pH, time, temperature, and adsorbate concentration.					
29807078	12	7	theme	constants	1518:1526	arg1	decrease					1484:1491	the decrease	1480:1491	the decrease of the adsorption energy constants (B) of Temkin model	1480:1546	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	4	8	theme	concentration	746:758	arg1	change					699:704	the change	695:704	the change of pH, time, temperature, and adsorbate concentration	695:758	The adsorption is checked for Methylene Blue and Acid Blue 25 in batch mode with reference to the change of pH, time, temperature, and adsorbate concentration.					
29807078	4	9	theme	temperature	719:729	arg1	change					699:704	the change	695:704	the change of pH, time, temperature, and adsorbate concentration	695:758	The adsorption is checked for Methylene Blue and Acid Blue 25 in batch mode with reference to the change of pH, time, temperature, and adsorbate concentration.					
29807078	4	10	theme	adsorbate	736:744	arg1	concentration					746:758	adsorbate concentration	736:758	adsorbate concentration	736:758	The adsorption is checked for Methylene Blue and Acid Blue 25 in batch mode with reference to the change of pH, time, temperature, and adsorbate concentration.					
29807078	3	11	theme	shapes	593:598	arg1	aggregates					549:558	aggregates	549:558	aggregates of different sizes and irregular shapes	549:598	The morphology of the alginate gel beads are changed after functionalization and are characterized by aggregates of different sizes and irregular shapes.					
29807078	9	12	theme	successive	1118:1127	arg1	stages					1140:1145	two successive adsorption stages	1114:1145	two successive adsorption stages of mass transport	1114:1163	The intra-particular Diffusion exhibits the existence of two successive adsorption stages of mass transport.					
29807078	0	13	theme	gel	134:136	arg1	beads					138:142	ethoxy-silica] composite gel beads	109:142	ethoxy-silica] composite gel beads	109:142	Improved removal of dyes by [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads.					
29807078	6	14	theme	sorption	828:835	arg1	capacities					837:846	The sorption capacities	824:846	The sorption capacities	824:846	The sorption capacities were improved using composite gel beads compared to gel beads.					
29807078	12	15	theme	model	1542:1546	arg1	B					1529:1529	B	1529:1529	B	1529:1529	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	12	15	theme	model	1542:1546	arg1	constants					1518:1526	the adsorption energy constants	1496:1526	the adsorption energy constants (B) of Temkin model	1496:1546	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	9	16	theme	adsorption	1129:1138	arg1	stages					1140:1145	two successive adsorption stages	1114:1145	two successive adsorption stages of mass transport	1114:1163	The intra-particular Diffusion exhibits the existence of two successive adsorption stages of mass transport.					
29807078	12	17	theme	enthalpy	1380:1387	arg1	value					1371:1375	The negative value	1358:1375	The negative value of enthalpy (-23.7 < ΔH* < -7.14)	1358:1409	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	11	18	theme	dyes	1344:1347	arg1	removal					1329:1335	the removal	1325:1335	the removal of the dyes (n > 1)	1325:1355	The prepared beads are considered moderate for the removal of the dyes (n > 1).					
29807078	7	19	theme	adsorbates	979:988	arg1	formation					930:938	A possible complex formation	911:938	A possible complex formation of composite gel beads and the studied adsorbates	911:988	A possible complex formation of composite gel beads and the studied adsorbates is proposed.					
29807078	9	20	theme	stages	1140:1145	arg1	existence					1101:1109	the existence	1097:1109	the existence of two successive adsorption stages of mass transport	1097:1163	The intra-particular Diffusion exhibits the existence of two successive adsorption stages of mass transport.					
29807078	3	21	theme	gel	478:480	arg1	beads					482:486	the alginate gel beads	465:486	the alginate gel beads	465:486	The morphology of the alginate gel beads are changed after functionalization and are characterized by aggregates of different sizes and irregular shapes.					
29807078	0	22	theme	Improved	0:7	arg1	removal					9:15	Improved removal	0:15	Improved removal of dyes by [sodium alginate/4-methyl-2-(naphthalen-2-yl)	0:72	Improved removal of dyes by [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads.					
29807078	3	23	theme	beads	482:486	arg1	morphology					451:460	The morphology	447:460	The morphology of the alginate gel beads	447:486	The morphology of the alginate gel beads are changed after functionalization and are characterized by aggregates of different sizes and irregular shapes.					
29807078	0	24	theme	dyes	20:23	arg1	removal					9:15	Improved removal	0:15	Improved removal of dyes by [sodium alginate/4-methyl-2-(naphthalen-2-yl)	0:72	Improved removal of dyes by [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads.					
29807078	11	25	theme	prepared	1282:1289	arg1	beads					1291:1295	The prepared beads	1278:1295	The prepared beads	1278:1295	The prepared beads are considered moderate for the removal of the dyes (n > 1).					
29807078	8	26	theme	second	1043:1048	arg1	order					1050:1054	the second order	1039:1054	the second order	1039:1054	The kinetic data complies well with the second order.					
29807078	9	27	theme	mass	1150:1153	arg1	transport					1155:1163	mass transport	1150:1163	mass transport	1150:1163	The intra-particular Diffusion exhibits the existence of two successive adsorption stages of mass transport.					
29807078	12	28	theme	energy	1511:1516	arg1	B					1529:1529	B	1529:1529	B	1529:1529	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	12	28	theme	energy	1511:1516	arg1	constants					1518:1526	the adsorption energy constants	1496:1526	the adsorption energy constants (B) of Temkin model	1496:1546	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	2	29	theme	point	410:414	arg1	charge					421:426	point zero charge	410:426	point zero charge	410:426	The characterizations were carried out using Fourier Transform InfraRed, Scanning Electron Microscopy, point zero charge and water content.					
29807078	9	30	theme	transport	1155:1163	arg1	stages					1140:1145	two successive adsorption stages	1114:1145	two successive adsorption stages of mass transport	1114:1163	The intra-particular Diffusion exhibits the existence of two successive adsorption stages of mass transport.					
29807078	1	31	theme	-N-propylpentanamide-functionalized	222:256	arg1	beads					287:291	[sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads	177:291	[sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads	177:291	In this paper, the synthesis of [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads is described.					
29807078	0	32	theme	[sodium	28:34	arg1	naphthalen-2-yl					57:71	naphthalen-2-yl	57:71	naphthalen-2-yl	57:71	Improved removal of dyes by [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads.					
29807078	0	32	theme	[sodium	28:34	arg1	alginate/4-methyl-2-					36:55	[sodium alginate/4-methyl-2-	28:55	[sodium alginate/4-methyl-2-(naphthalen-2-yl)	28:72	Improved removal of dyes by [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads.					
29807078	12	33	theme	adsorption	1500:1509	arg1	B					1529:1529	B	1529:1529	B	1529:1529	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	12	33	theme	adsorption	1500:1509	arg1	constants					1518:1526	the adsorption energy constants	1496:1526	the adsorption energy constants (B) of Temkin model	1496:1546	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	4	34	with	mode	672:675	arg1	reference					682:690	reference	682:690	reference to the change of pH, time, temperature, and adsorbate concentration	682:758	The adsorption is checked for Methylene Blue and Acid Blue 25 in batch mode with reference to the change of pH, time, temperature, and adsorbate concentration.					
29807078	2	35	theme	water	432:436	arg1	content					438:444	water content	432:444	water content	432:444	The characterizations were carried out using Fourier Transform InfraRed, Scanning Electron Microscopy, point zero charge and water content.					
29807078	1	36	theme	ethoxy-silica	258:270	arg1	beads					287:291	[sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads	177:291	[sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads	177:291	In this paper, the synthesis of [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads is described.					
29807078	3	37	theme	alginate	469:476	arg1	beads					482:486	the alginate gel beads	465:486	the alginate gel beads	465:486	The morphology of the alginate gel beads are changed after functionalization and are characterized by aggregates of different sizes and irregular shapes.					
29807078	10	38	theme	adsorption	1260:1269	arg1	sites					1271:1275	the heterogeneous adsorption sites	1242:1275	the heterogeneous adsorption sites	1242:1275	The data fitted with Freundlich indicate that the adsorption might occur in the heterogeneous adsorption sites.					
29807078	6	39	theme	gel	900:902	arg1	beads					904:908	gel beads	900:908	gel beads	900:908	The sorption capacities were improved using composite gel beads compared to gel beads.					
29807078	5	40	from	reliance	763:770	arg1	conditions					800:809	the studied experimental conditions	775:809	the studied experimental conditions	775:809	A reliance on the studied experimental conditions is observed.					
29807078	12	41	theme	-23.7 < ΔH	1390:1399	arg1	enthalpy					1380:1387	enthalpy	1380:1387	enthalpy (-23.7 < ΔH* < -7.14)	1380:1409	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	12	41	theme	-23.7 < ΔH	1390:1399	arg1	*					1400:1400	-23.7 < ΔH* < -7.14	1390:1408	-23.7 < ΔH* < -7.14	1390:1408	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	10	42	theme	heterogeneous	1246:1258	arg1	sites					1271:1275	the heterogeneous adsorption sites	1242:1275	the heterogeneous adsorption sites	1242:1275	The data fitted with Freundlich indicate that the adsorption might occur in the heterogeneous adsorption sites.					
29807078	2	43	dep	Transform	360:368	arg1	InfraRed					370:377	InfraRed	370:377	Transform InfraRed	360:377	The characterizations were carried out using Fourier Transform InfraRed, Scanning Electron Microscopy, point zero charge and water content.					
29807078	7	44	theme	studied	971:977	arg1	adsorbates					979:988	the studied adsorbates	967:988	the studied adsorbates	967:988	A possible complex formation of composite gel beads and the studied adsorbates is proposed.					
29807078	2	45	dep	Fourier	352:358	arg1	Transform					360:368	Transform	360:368	Transform InfraRed	360:377	The characterizations were carried out using Fourier Transform InfraRed, Scanning Electron Microscopy, point zero charge and water content.					
29807078	7	46	theme	gel	953:955	arg1	beads					957:961	composite gel beads	943:961	composite gel beads	943:961	A possible complex formation of composite gel beads and the studied adsorbates is proposed.					
29807078	4	47	theme	pH	709:710	arg1	change					699:704	the change	695:704	the change of pH, time, temperature, and adsorbate concentration	695:758	The adsorption is checked for Methylene Blue and Acid Blue 25 in batch mode with reference to the change of pH, time, temperature, and adsorbate concentration.					
29807078	12	48	theme	Temkin	1535:1540	arg1	model					1542:1546	Temkin model	1535:1546	Temkin model	1535:1546	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	4	49	theme	time	713:716	arg1	change					699:704	the change	695:704	the change of pH, time, temperature, and adsorbate concentration	695:758	The adsorption is checked for Methylene Blue and Acid Blue 25 in batch mode with reference to the change of pH, time, temperature, and adsorbate concentration.					
29807078	2	50	theme	Electron	389:396	arg1	Microscopy					398:407	Scanning Electron Microscopy	380:407	Scanning Electron Microscopy	380:407	The characterizations were carried out using Fourier Transform InfraRed, Scanning Electron Microscopy, point zero charge and water content.					
29807078	7	51	theme	beads	957:961	arg1	formation					930:938	A possible complex formation	911:938	A possible complex formation of composite gel beads and the studied adsorbates	911:988	A possible complex formation of composite gel beads and the studied adsorbates is proposed.					
29807078	1	52	theme	composite	273:281	arg1	beads					287:291	[sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads	177:291	[sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads	177:291	In this paper, the synthesis of [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads is described.					
29807078	6	53	theme	gel	878:880	arg1	beads					882:886	composite gel beads	868:886	composite gel beads compared to gel beads	868:908	The sorption capacities were improved using composite gel beads compared to gel beads.					
29807078	2	54	theme	Scanning	380:387	arg1	Microscopy					398:407	Scanning Electron Microscopy	380:407	Scanning Electron Microscopy	380:407	The characterizations were carried out using Fourier Transform InfraRed, Scanning Electron Microscopy, point zero charge and water content.					
29807078	9	55	theme	intra-particular	1061:1076	arg1	Diffusion					1078:1086	The intra-particular Diffusion	1057:1086	The intra-particular Diffusion	1057:1086	The intra-particular Diffusion exhibits the existence of two successive adsorption stages of mass transport.					
29807078	1	56	theme	gel	283:285	arg1	beads					287:291	[sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads	177:291	[sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads	177:291	In this paper, the synthesis of [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads is described.					
29807078	5	57	theme	studied	779:785	arg1	conditions					800:809	the studied experimental conditions	775:809	the studied experimental conditions	775:809	A reliance on the studied experimental conditions is observed.					
29807078	6	58	theme	composite	868:876	arg1	beads					882:886	composite gel beads	868:886	composite gel beads compared to gel beads	868:908	The sorption capacities were improved using composite gel beads compared to gel beads.					
29807078	7	59	theme	composite	943:951	arg1	beads					957:961	composite gel beads	943:961	composite gel beads	943:961	A possible complex formation of composite gel beads and the studied adsorbates is proposed.					
29807078	1	60	theme	beads	287:291	arg1	synthesis					164:172	the synthesis	160:172	the synthesis of [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads	160:291	In this paper, the synthesis of [sodium alginate/4-methyl-2-(naphthalen-2-yl)-N-propylpentanamide-functionalized ethoxy-silica] composite gel beads is described.					
29807078	5	61	theme	experimental	787:798	arg1	conditions					800:809	the studied experimental conditions	775:809	the studied experimental conditions	775:809	A reliance on the studied experimental conditions is observed.					
29807078	12	62	theme	negative	1362:1369	arg1	value					1371:1375	The negative value	1358:1375	The negative value of enthalpy (-23.7 < ΔH* < -7.14)	1358:1409	The negative value of enthalpy (-23.7 < ΔH* < -7.14) confirms that the process is exothermic which is, also, supported by the decrease of the adsorption energy constants (B) of Temkin model.					
29807078	3	63	theme	irregular	583:591	arg1	shapes					593:598	irregular shapes	583:598	irregular shapes	583:598	The morphology of the alginate gel beads are changed after functionalization and are characterized by aggregates of different sizes and irregular shapes.					
29807078	7	64	theme	complex	922:928	arg1	formation					930:938	A possible complex formation	911:938	A possible complex formation of composite gel beads and the studied adsorbates	911:988	A possible complex formation of composite gel beads and the studied adsorbates is proposed.					
29807078	4	65	theme	batch	666:670	arg1	mode					672:675	batch mode	666:675	batch mode with reference to the change of pH, time, temperature, and adsorbate concentration	666:758	The adsorption is checked for Methylene Blue and Acid Blue 25 in batch mode with reference to the change of pH, time, temperature, and adsorbate concentration.					
30539399	5	0	theme	optimum	746:752	arg1	conditions					754:763	the optimum conditions	742:763	the optimum conditions (pH = 6, 55 °C, 48 h)	742:785	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	2	1	contain	possess	425:431	arg1	CS/LDHs					411:417	CS/LDHs	411:417	CS/LDHs	411:417	The structure of CS/LDHs was characterized by SEM, FTIR, and XRD, which proved that chitosan (CS) was successfully induced into hydrotalcite and CS/LDHs still possess the structural characteristics of hydrotalcite.					
30539399	2	1	contain	possess	425:431	arg2	characteristics					448:462	the structural characteristics	433:462	the structural characteristics of hydrotalcite	433:478	The structure of CS/LDHs was characterized by SEM, FTIR, and XRD, which proved that chitosan (CS) was successfully induced into hydrotalcite and CS/LDHs still possess the structural characteristics of hydrotalcite.					
30539399	2	1	contain	possess	425:431	arg1	hydrotalcite					394:405	hydrotalcite	394:405	hydrotalcite	394:405	The structure of CS/LDHs was characterized by SEM, FTIR, and XRD, which proved that chitosan (CS) was successfully induced into hydrotalcite and CS/LDHs still possess the structural characteristics of hydrotalcite.					
30539399	7	2	theme	removal	1218:1224	arg1	L-LDHs					1194:1199	L-LDHs	1194:1199	L-LDHs	1194:1199	After 5 cycles, it maintained 67% (L-CS/LDHs) and 54% (L-LDHs) of the original removal.					
30539399	7	2	theme	removal	1218:1224	arg1	L-CS/LDHs					1174:1182	L-CS/LDHs	1174:1182	L-CS/LDHs	1174:1182	After 5 cycles, it maintained 67% (L-CS/LDHs) and 54% (L-LDHs) of the original removal.					
30539399	7	2	theme	removal	1218:1224	arg1	removal					1218:1224	the original removal	1205:1224	the original removal	1205:1224	After 5 cycles, it maintained 67% (L-CS/LDHs) and 54% (L-LDHs) of the original removal.					
30539399	7	2	theme	removal	1218:1224	arg1	%					1171:1171	67%	1169:1171	67% (L-CS/LDHs)	1169:1183	After 5 cycles, it maintained 67% (L-CS/LDHs) and 54% (L-LDHs) of the original removal.					
30539399	7	2	theme	removal	1218:1224	arg1	%					1191:1191	54%	1189:1191	54% (L-LDHs) of the original removal	1189:1224	After 5 cycles, it maintained 67% (L-CS/LDHs) and 54% (L-LDHs) of the original removal.					
30539399	4	3	theme	immobilized	603:613	arg1	L-CS/LDHs					624:632	L-CS/LDHs	624:632	L-CS/LDHs	624:632	The activity of immobilized laccase (L-CS/LDHs) with CS/LDHs as carrier is significantly better than that of the one (L-LDHs) using LDHs as carrier.					
30539399	4	3	theme	immobilized	603:613	arg1	laccase					615:621	immobilized laccase	603:621	immobilized laccase (L-CS/LDHs)	603:633	The activity of immobilized laccase (L-CS/LDHs) with CS/LDHs as carrier is significantly better than that of the one (L-LDHs) using LDHs as carrier.					
30539399	6	4	theme	Co2+	1069:1072	arg1	laccase					1030:1036	laccase	1030:1036	laccase	1030:1036	It includes the catalytic action of laccase and dechlorination of Fe3+ and Co2+, and the adsorption can be ignored under the optimal conditions.					
30539399	6	4	theme	Co2+	1069:1072	arg1	dechlorination					1042:1055	dechlorination	1042:1055	dechlorination	1042:1055	It includes the catalytic action of laccase and dechlorination of Fe3+ and Co2+, and the adsorption can be ignored under the optimal conditions.					
30539399	6	5	theme	dechlorination	1042:1055	arg1	action					1020:1025	the catalytic action	1006:1025	the catalytic action of laccase and dechlorination of Fe3+ and Co2+	1006:1072	It includes the catalytic action of laccase and dechlorination of Fe3+ and Co2+, and the adsorption can be ignored under the optimal conditions.					
30539399	5	6	theme	2,4-DCP	901:907	arg1	removal					890:896	the removal	886:896	the removal of 2,4-DCP by L-CS/LDHs	886:920	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	5	6	theme	2,4-DCP	901:907	arg1	excellent					925:933	excellent	925:933	excellent	925:933	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	1	7	theme	Chitosan/Co-Fe-layered	109:130	arg1	CS/LDHs					151:157	CS/LDHs	151:157	CS/LDHs	151:157	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	1	7	theme	Chitosan/Co-Fe-layered	109:130	arg1	hydroxides					139:148	Chitosan/Co-Fe-layered double hydroxides	109:148	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs)	109:158	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	2	8	theme	structural	437:446	arg1	characteristics					448:462	the structural characteristics	433:462	the structural characteristics of hydrotalcite	433:478	The structure of CS/LDHs was characterized by SEM, FTIR, and XRD, which proved that chitosan (CS) was successfully induced into hydrotalcite and CS/LDHs still possess the structural characteristics of hydrotalcite.					
30539399	1	9	theme	double	132:137	arg1	CS/LDHs					151:157	CS/LDHs	151:157	CS/LDHs	151:157	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	1	9	theme	double	132:137	arg1	hydroxides					139:148	Chitosan/Co-Fe-layered double hydroxides	109:148	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs)	109:158	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	0	10	theme	double	38:43	arg1	hydroxides					45:54	chitosan/Co-Fe-layered double hydroxides	15:54	chitosan/Co-Fe-layered double hydroxides	15:54	Preparation of chitosan/Co-Fe-layered double hydroxides and its performance for removing 2,4-dichlorophenol.					
30539399	1	11	with	material	230:237	arg1	properties					254:263	excellent properties	244:263	excellent properties	244:263	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	6	12	theme	Fe3+	1060:1063	arg1	laccase					1030:1036	laccase	1030:1036	laccase	1030:1036	It includes the catalytic action of laccase and dechlorination of Fe3+ and Co2+, and the adsorption can be ignored under the optimal conditions.					
30539399	6	12	theme	Fe3+	1060:1063	arg1	dechlorination					1042:1055	dechlorination	1042:1055	dechlorination	1042:1055	It includes the catalytic action of laccase and dechlorination of Fe3+ and Co2+, and the adsorption can be ignored under the optimal conditions.					
30539399	0	13	theme	chitosan/Co-Fe-layered	15:36	arg1	hydroxides					45:54	chitosan/Co-Fe-layered double hydroxides	15:54	chitosan/Co-Fe-layered double hydroxides	15:54	Preparation of chitosan/Co-Fe-layered double hydroxides and its performance for removing 2,4-dichlorophenol.					
30539399	5	14	dep	conditions	754:763	arg1	48 h					781:784	48 h	781:784	48 h	781:784	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	5	14	dep	conditions	754:763	arg1	55 °C					774:778	55 °C	774:778	55 °C	774:778	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	5	14	dep	conditions	754:763	arg1	pH = 6					766:771	pH = 6	766:771	pH = 6	766:771	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	3	15	theme	adsorbent	565:573	arg1	CS/LDHs					545:551	CS/LDHs	545:551	CS/LDHs	545:551	The adsorption of 2,4-dichlorophenol (2,4-DCP) was studied with CS/LDHs and LDHs as adsorbent separately.					
30539399	1	16	theme	composite	220:228	arg1	material					230:237	composite material	220:237	composite material with excellent properties	220:263	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	5	17	theme	better	808:813	arg1	performance					823:833	better removal performance	808:833	better removal performance for 2,4-DCP	808:845	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	1	18	theme	material	230:237	arg1	kind					212:215	a kind	210:215	a kind of composite material with excellent properties	210:263	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	1	18	theme	material	230:237	arg1	method					193:198	coprecipitation method	177:198	coprecipitation method	177:198	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	0	19	theme	hydroxides	45:54	arg1	Preparation					0:10	Preparation	0:10	Preparation of chitosan/Co-Fe-layered double hydroxides	0:54	Preparation of chitosan/Co-Fe-layered double hydroxides and its performance for removing 2,4-dichlorophenol.					
30539399	0	19	theme	hydroxides	45:54	arg1	performance					64:74	its performance	60:74	its performance for removing 2,4-dichlorophenol	60:106	Preparation of chitosan/Co-Fe-layered double hydroxides and its performance for removing 2,4-dichlorophenol.					
30539399	5	20	theme	removal	815:821	arg1	performance					823:833	better removal performance	808:833	better removal performance for 2,4-DCP	808:845	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	2	21	theme	hydrotalcite	467:478	arg1	characteristics					448:462	the structural characteristics	433:462	the structural characteristics of hydrotalcite	433:478	The structure of CS/LDHs was characterized by SEM, FTIR, and XRD, which proved that chitosan (CS) was successfully induced into hydrotalcite and CS/LDHs still possess the structural characteristics of hydrotalcite.					
30539399	6	22	theme	optimal	1119:1125	arg1	conditions					1127:1136	the optimal conditions	1115:1136	the optimal conditions	1115:1136	It includes the catalytic action of laccase and dechlorination of Fe3+ and Co2+, and the adsorption can be ignored under the optimal conditions.					
30539399	3	23	theme	2,4-dichlorophenol	499:516	arg1	adsorption					485:494	The adsorption	481:494	The adsorption of 2,4-dichlorophenol (2,4-DCP)	481:526	The adsorption of 2,4-dichlorophenol (2,4-DCP) was studied with CS/LDHs and LDHs as adsorbent separately.					
30539399	7	24	theme	original	1209:1216	arg1	removal					1218:1224	the original removal	1205:1224	the original removal	1205:1224	After 5 cycles, it maintained 67% (L-CS/LDHs) and 54% (L-LDHs) of the original removal.					
30539399	6	25	theme	laccase	1030:1036	arg1	action					1020:1025	the catalytic action	1006:1025	the catalytic action of laccase and dechlorination of Fe3+ and Co2+	1006:1072	It includes the catalytic action of laccase and dechlorination of Fe3+ and Co2+, and the adsorption can be ignored under the optimal conditions.					
30539399	1	26	theme	excellent	244:252	arg1	properties					254:263	excellent properties	244:263	excellent properties	244:263	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	5	27	theme	initial	957:963	arg1	concentration					965:977	its initial concentration	953:977	its initial concentration below 60 mg/L	953:991	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	2	28	theme	CS/LDHs	283:289	arg1	structure					270:278	The structure	266:278	The structure of CS/LDHs	266:289	The structure of CS/LDHs was characterized by SEM, FTIR, and XRD, which proved that chitosan (CS) was successfully induced into hydrotalcite and CS/LDHs still possess the structural characteristics of hydrotalcite.					
30539399	6	29	theme	catalytic	1010:1018	arg1	action					1020:1025	the catalytic action	1006:1025	the catalytic action of laccase and dechlorination of Fe3+ and Co2+	1006:1072	It includes the catalytic action of laccase and dechlorination of Fe3+ and Co2+, and the adsorption can be ignored under the optimal conditions.					
30539399	4	30	theme	laccase	615:621	arg1	better					676:681	better	676:681	better	676:681	The activity of immobilized laccase (L-CS/LDHs) with CS/LDHs as carrier is significantly better than that of the one (L-LDHs) using LDHs as carrier.					
30539399	4	30	theme	laccase	615:621	arg1	activity					591:598	The activity	587:598	The activity of immobilized laccase (L-CS/LDHs) with CS/LDHs as carrier	587:657	The activity of immobilized laccase (L-CS/LDHs) with CS/LDHs as carrier is significantly better than that of the one (L-LDHs) using LDHs as carrier.					
30539399	4	31	with	activity	591:598	arg1	CS/LDHs					640:646	CS/LDHs	640:646	CS/LDHs as carrier	640:657	The activity of immobilized laccase (L-CS/LDHs) with CS/LDHs as carrier is significantly better than that of the one (L-LDHs) using LDHs as carrier.					
30539399	5	32	dep	exhibited	798:806	arg1	100 mg/L					856:863	100 mg/L	856:863	100 mg/L	856:863	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	5	32	dep	exhibited	798:806	arg1	%					853:853	81.53%	848:853	81.53%	848:853	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	5	32	dep	exhibited	798:806	arg1	%					882:882	63.55%	877:882	63.55%	877:882	Under the optimum conditions (pH = 6, 55 °C, 48 h), L-CS/LDHs exhibited better removal performance for 2,4-DCP (81.53%, 100 mg/L) than LDHs (63.55%); the removal of 2,4-DCP by L-CS/LDHs is excellent, exceeding 97% as its initial concentration below 60 mg/L.					
30539399	1	33	theme	coprecipitation	177:191	arg1	kind					212:215	a kind	210:215	a kind of composite material with excellent properties	210:263	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
30539399	1	33	theme	coprecipitation	177:191	arg1	method					193:198	coprecipitation method	177:198	coprecipitation method	177:198	Chitosan/Co-Fe-layered double hydroxides (CS/LDHs) were prepared by coprecipitation method, which is a kind of composite material with excellent properties.					
29659664	6	0	dep	Results	698:704	arg1	found					709:713	found	709:713	found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period	709:820	Key Results We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period.					
29659664	14	1	theme	tube	1986:1989	arg1	growth					1991:1996	pollen tube growth	1979:1996	pollen tube growth	1979:1996	The evidence that different aspects of pollen tube growth are affected is an indication that pollen tubes adapt to metabolic stress.					
29659664	8	2	theme	different	1095:1103	arg1	distribution					1105:1116	a different distribution	1093:1116	a different distribution in pollen tubes grown in sucrose-depleted media	1093:1164	The intracellular pH and ROS levels also showed a different distribution in pollen tubes grown in sucrose-depleted media.					
29659664	12	3	theme	Pollen	1679:1684	arg1	tubes					1686:1690	Conclusion Pollen tubes	1668:1690	Conclusion Pollen tubes	1668:1690	Conclusion Pollen tubes represent an ideal model system for studying metabolic pathways during the growth of plant cells.					
29659664	10	4	theme	oscillatory	1491:1501	arg1	growth					1503:1508	oscillatory growth	1491:1508	oscillatory growth	1491:1508	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	11	5	theme	generative	1600:1609	arg1	cells					1611:1615	generative cells	1600:1615	generative cells	1600:1615	Growth in glycerol-based media also temporarily affected the movement of generative cells and, in parallel, the deposition of callose plugs.					
29659664	5	6	theme	related	619:625	arg1	composition					657:667	metabolite composition	646:667	metabolite composition	646:667	We focused on cell growth and related processes, such as metabolite composition and cell wall synthesis.					
29659664	5	6	theme	related	619:625	arg1	processes					627:635	related processes	619:635	related processes	619:635	We focused on cell growth and related processes, such as metabolite composition and cell wall synthesis.					
29659664	5	6	theme	related	619:625	arg1	synthesis					683:691	cell wall synthesis	673:691	cell wall synthesis	673:691	We focused on cell growth and related processes, such as metabolite composition and cell wall synthesis.					
29659664	6	7	theme	pollen	748:753	arg1	germination					755:765	pollen germination	748:765	pollen germination	748:765	Key Results We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period.					
29659664	6	8	theme	sucrose	732:738	arg1	lack					724:727	the lack	720:727	the lack of sucrose	720:738	Key Results We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period.					
29659664	13	9	theme	energetic	1844:1852	arg1	source					1854:1859	a less energetic source	1837:1859	a less energetic source for cell growth than sucrose	1837:1888	In our study, we found evidence that glycerol, a less energetic source for cell growth than sucrose, causes critical changes in cell wall deposition.					
29659664	13	9	theme	energetic	1844:1852	arg1	glycerol					1827:1834	glycerol	1827:1834	glycerol	1827:1834	In our study, we found evidence that glycerol, a less energetic source for cell growth than sucrose, causes critical changes in cell wall deposition.					
29659664	2	10	theme	storage	273:279	arg1	sources					281:287	internal and external storage sources	251:287	internal and external storage sources	251:287	Energy can derive from internal and external storage sources.					
29659664	1	11	theme	high	188:191	arg1	rates					193:197	high rates	188:197	high rates of energy	188:207	Background and Aims Pollen tubes are rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth.					
29659664	5	12	theme	cell	673:676	arg1	synthesis					683:691	cell wall synthesis	673:691	cell wall synthesis	673:691	We focused on cell growth and related processes, such as metabolite composition and cell wall synthesis.					
29659664	9	13	theme	wall	1206:1209	arg1	level					1211:1215	the cell wall level	1197:1215	the cell wall level	1197:1215	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	7	14	theme	glycerol-based	933:946	arg1	media					948:952	glycerol-based media	933:952	glycerol-based media	933:952	Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose.					
29659664	3	15	theme	carbon	302:307	arg1	sources					309:315	carbon sources	302:315	carbon sources	302:315	The lack of carbon sources can cause various problems during pollen tube growth, which in turn could affect the reproduction of plants.					
29659664	11	16	theme	callose	1653:1659	arg1	plugs					1661:1665	callose plugs	1653:1665	callose plugs	1653:1665	Growth in glycerol-based media also temporarily affected the movement of generative cells and, in parallel, the deposition of callose plugs.					
29659664	0	17	theme	growth	48:53	arg1	mechanism					55:63	the tip growth mechanism	40:63	the tip growth mechanism of tobacco pollen tubes	40:87	Depletion of sucrose induces changes in the tip growth mechanism of tobacco pollen tubes.					
29659664	3	18	theme	various	327:333	arg1	problems					335:342	various problems	327:342	various problems	327:342	The lack of carbon sources can cause various problems during pollen tube growth, which in turn could affect the reproduction of plants.					
29659664	4	19	theme	deficiency	468:477	arg1	effects					450:456	the effects	446:456	the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes	446:530	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	0	20	theme	pollen	76:81	arg1	tubes					83:87	tobacco pollen tubes	68:87	tobacco pollen tubes	68:87	Depletion of sucrose induces changes in the tip growth mechanism of tobacco pollen tubes.					
29659664	10	21	theme	wall	1400:1403	arg1	material					1405:1412	newly secreted cell wall material	1380:1412	newly secreted cell wall material (mainly pectins)	1380:1429	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	7	22	theme	sucrose	884:890	arg1	shortage					892:899	sucrose shortage	884:899	sucrose shortage	884:899	Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose.					
29659664	3	23	theme	tube	358:361	arg1	growth					363:368	pollen tube growth	351:368	pollen tube growth	351:368	The lack of carbon sources can cause various problems during pollen tube growth, which in turn could affect the reproduction of plants.					
29659664	10	24	theme	secreted	1386:1393	arg1	material					1405:1412	newly secreted cell wall material	1380:1412	newly secreted cell wall material (mainly pectins)	1380:1429	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	1	25	dep	growing	135:141	arg1	inactive					163:170	inactive	163:170	inactive	163:170	Background and Aims Pollen tubes are rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth.					
29659664	7	26	theme	UDP-glucose	1032:1042	arg1	concentrations					992:1005	the concentrations	988:1005	the concentrations of glucose, fructose and UDP-glucose	988:1042	Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose.					
29659664	5	27	theme	wall	678:681	arg1	synthesis					683:691	cell wall synthesis	673:691	cell wall synthesis	673:691	We focused on cell growth and related processes, such as metabolite composition and cell wall synthesis.					
29659664	4	28	theme	tabacum	511:517	arg1	tubes					526:530	Nicotiana tabacum pollen tubes	501:530	Nicotiana tabacum pollen tubes	501:530	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	0	29	theme	sucrose	13:19	arg1	Depletion					0:8	Depletion	0:8	Depletion of sucrose	0:19	Depletion of sucrose induces changes in the tip growth mechanism of tobacco pollen tubes.					
29659664	9	30	theme	related	1278:1284	arg1	enzymes					1270:1276	two enzymes	1266:1276	two enzymes related to cell wall synthesis (sucrose synthase and callose synthase)	1266:1347	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	12	31	theme	cells	1783:1787	arg1	growth					1767:1772	the growth	1763:1772	the growth of plant cells	1763:1787	Conclusion Pollen tubes represent an ideal model system for studying metabolic pathways during the growth of plant cells.					
29659664	4	32	theme	tubes	526:530	arg1	development					486:496	the development	482:496	the development of Nicotiana tabacum pollen tubes	482:530	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	14	33	theme	different	1958:1966	arg1	aspects					1968:1974	different aspects	1958:1974	different aspects of pollen tube growth	1958:1996	The evidence that different aspects of pollen tube growth are affected is an indication that pollen tubes adapt to metabolic stress.					
29659664	9	34	theme	cell	1289:1292	arg1	synthesis					1299:1307	cell wall synthesis	1289:1307	cell wall synthesis (sucrose synthase and callose synthase)	1289:1347	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	6	35	theme	specific	799:806	arg1	period					815:820	a specific growth period	797:820	a specific growth period	797:820	Key Results We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period.					
29659664	12	36	theme	metabolic	1737:1745	arg1	pathways					1747:1754	metabolic pathways	1737:1754	metabolic pathways	1737:1754	Conclusion Pollen tubes represent an ideal model system for studying metabolic pathways during the growth of plant cells.					
29659664	10	37	theme	altered	1441:1447	arg1	distribution					1449:1460	an altered distribution	1438:1460	an altered distribution corresponding to the lack of oscillatory growth in pollen tubes	1438:1524	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	13	38	theme	wall	1923:1926	arg1	deposition					1928:1937	cell wall deposition	1918:1937	cell wall deposition	1918:1937	In our study, we found evidence that glycerol, a less energetic source for cell growth than sucrose, causes critical changes in cell wall deposition.					
29659664	9	39	theme	sucrose	1310:1316	arg1	synthase					1318:1325	sucrose synthase	1310:1325	sucrose synthase	1310:1325	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	6	40	theme	tube	778:781	arg1	length					783:788	pollen tube length	771:788	pollen tube length	771:788	Key Results We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period.					
29659664	12	41	theme	model	1711:1715	arg1	system					1717:1722	an ideal model system	1702:1722	an ideal model system for studying metabolic pathways during the growth of plant cells	1702:1787	Conclusion Pollen tubes represent an ideal model system for studying metabolic pathways during the growth of plant cells.					
29659664	1	42	dep	Background	90:99	arg1	tubes					117:121	Pollen tubes	110:121	Pollen tubes	110:121	Background and Aims Pollen tubes are rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth.					
29659664	7	43	from	decreases	975:983	arg1	concentrations					992:1005	the concentrations	988:1005	the concentrations of glucose, fructose and UDP-glucose	988:1042	Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose.					
29659664	7	44	theme	ATP	849:851	arg1	concentration					853:865	ATP concentration	849:865	ATP concentration	849:865	Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose.					
29659664	8	45	theme	ROS	1070:1072	arg1	levels					1074:1079	ROS levels	1070:1079	ROS levels	1070:1079	The intracellular pH and ROS levels also showed a different distribution in pollen tubes grown in sucrose-depleted media.					
29659664	11	46	theme	plugs	1661:1665	arg1	deposition					1639:1648	the deposition	1635:1648	the deposition of callose plugs	1635:1665	Growth in glycerol-based media also temporarily affected the movement of generative cells and, in parallel, the deposition of callose plugs.					
29659664	4	47	theme	growth	574:579	arg1	medium					581:586	the growth medium	570:586	the growth medium	570:586	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	5	48	theme	cell	603:606	arg1	growth					608:613	cell growth	603:613	cell growth	603:613	We focused on cell growth and related processes, such as metabolite composition and cell wall synthesis.					
29659664	13	49	theme	critical	1898:1905	arg1	changes					1907:1913	critical changes	1898:1913	critical changes	1898:1913	In our study, we found evidence that glycerol, a less energetic source for cell growth than sucrose, causes critical changes in cell wall deposition.					
29659664	4	50	dep	Methods	426:432	arg1	analysed					437:444	analysed	437:444	analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium	437:586	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	10	51	theme	growth	1503:1508	arg1	lack					1483:1486	the lack	1479:1486	the lack of oscillatory growth in pollen tubes	1479:1524	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	13	52	theme	cell	1865:1868	arg1	growth					1870:1875	cell growth	1865:1875	cell growth than sucrose	1865:1888	In our study, we found evidence that glycerol, a less energetic source for cell growth than sucrose, causes critical changes in cell wall deposition.					
29659664	2	53	theme	external	264:271	arg1	sources					281:287	internal and external storage sources	251:287	internal and external storage sources	251:287	Energy can derive from internal and external storage sources.					
29659664	11	54	theme	cells	1611:1615	arg1	movement					1588:1595	the movement	1584:1595	the movement of generative cells	1584:1615	Growth in glycerol-based media also temporarily affected the movement of generative cells and, in parallel, the deposition of callose plugs.					
29659664	4	55	from	effects	450:456	arg1	development					486:496	the development	482:496	the development of Nicotiana tabacum pollen tubes	482:530	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	2	56	theme	internal	251:258	arg1	sources					281:287	internal and external storage sources	251:287	internal and external storage sources	251:287	Energy can derive from internal and external storage sources.					
29659664	11	57	from	Growth	1527:1532	arg1	media					1552:1556	glycerol-based media	1537:1556	glycerol-based media	1537:1556	Growth in glycerol-based media also temporarily affected the movement of generative cells and, in parallel, the deposition of callose plugs.					
29659664	4	58	theme	Nicotiana	501:509	arg1	tabacum					511:517	Nicotiana tabacum	501:517	Nicotiana tabacum pollen tubes	501:530	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	7	59	theme	fructose	1019:1026	arg1	concentrations					992:1005	the concentrations	988:1005	the concentrations of glucose, fructose and UDP-glucose	988:1042	Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose.					
29659664	8	60	theme	pollen	1121:1126	arg1	tubes					1128:1132	pollen tubes	1121:1132	pollen tubes grown in sucrose-depleted media	1121:1164	The intracellular pH and ROS levels also showed a different distribution in pollen tubes grown in sucrose-depleted media.					
29659664	12	61	theme	Conclusion	1668:1677	arg1	tubes					1686:1690	Conclusion Pollen tubes	1668:1690	Conclusion Pollen tubes	1668:1690	Conclusion Pollen tubes represent an ideal model system for studying metabolic pathways during the growth of plant cells.					
29659664	10	62	theme	pollen	1513:1518	arg1	tubes					1520:1524	pollen tubes	1513:1524	pollen tubes	1513:1524	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	5	63	theme	metabolite	646:655	arg1	composition					657:667	metabolite composition	646:667	metabolite composition	646:667	We focused on cell growth and related processes, such as metabolite composition and cell wall synthesis.					
29659664	8	64	theme	sucrose-depleted	1143:1158	arg1	media					1160:1164	sucrose-depleted media	1143:1164	sucrose-depleted media	1143:1164	The intracellular pH and ROS levels also showed a different distribution in pollen tubes grown in sucrose-depleted media.					
29659664	14	65	theme	metabolic	2055:2063	arg1	stress					2065:2070	metabolic stress	2055:2070	metabolic stress	2055:2070	The evidence that different aspects of pollen tube growth are affected is an indication that pollen tubes adapt to metabolic stress.					
29659664	14	66	theme	pollen	2033:2038	arg1	tubes					2040:2044	pollen tubes	2033:2044	pollen tubes	2033:2044	The evidence that different aspects of pollen tube growth are affected is an indication that pollen tubes adapt to metabolic stress.					
29659664	4	67	from	glycerol	558:565	arg1	medium					581:586	the growth medium	570:586	the growth medium	570:586	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	7	68	theme	glucose	1010:1016	arg1	concentrations					992:1005	the concentrations	988:1005	the concentrations of glucose, fructose and UDP-glucose	988:1042	Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose.					
29659664	10	69	from	lack	1483:1486	arg1	tubes					1520:1524	pollen tubes	1513:1524	pollen tubes	1513:1524	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	9	70	theme	cell	1201:1204	arg1	level					1211:1215	the cell wall level	1197:1215	the cell wall level	1197:1215	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	1	71	theme	Pollen	110:115	arg1	tubes					117:121	Pollen tubes	110:121	Pollen tubes	110:121	Background and Aims Pollen tubes are rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth.					
29659664	3	72	theme	sources	309:315	arg1	lack					294:297	The lack	290:297	The lack of carbon sources	290:315	The lack of carbon sources can cause various problems during pollen tube growth, which in turn could affect the reproduction of plants.					
29659664	4	73	theme	energy	461:466	arg1	deficiency					468:477	energy deficiency	461:477	energy deficiency	461:477	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	1	74	theme	energy	202:207	arg1	rates					193:197	high rates	188:197	high rates of energy	188:207	Background and Aims Pollen tubes are rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth.					
29659664	10	75	dep	material	1405:1412	arg1	pectins					1422:1428	pectins	1422:1428	pectins	1422:1428	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	0	76	theme	tobacco	68:74	arg1	tubes					83:87	tobacco pollen tubes	68:87	tobacco pollen tubes	68:87	Depletion of sucrose induces changes in the tip growth mechanism of tobacco pollen tubes.					
29659664	1	77	theme	growing	135:141	arg1	cells					172:176	rapidly growing, photosynthetically inactive cells	127:176	rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth	127:225	Background and Aims Pollen tubes are rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth.					
29659664	1	77	theme	growing	135:141	arg1	Aims					105:108	Aims	105:108	Aims	105:108	Background and Aims Pollen tubes are rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth.					
29659664	1	77	theme	growing	135:141	arg1	Background					90:99	Background	90:99	Background	90:99	Background and Aims Pollen tubes are rapidly growing, photosynthetically inactive cells that need high rates of energy to support growth.					
29659664	0	78	theme	tubes	83:87	arg1	mechanism					55:63	the tip growth mechanism	40:63	the tip growth mechanism of tobacco pollen tubes	40:87	Depletion of sucrose induces changes in the tip growth mechanism of tobacco pollen tubes.					
29659664	6	79	theme	Key	694:696	arg1	Results					698:704	Key Results	694:704	Key Results We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period.	694:821	Key Results We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period.					
29659664	9	80	dep	synthesis	1299:1307	arg1	synthase					1318:1325	sucrose synthase	1310:1325	sucrose synthase	1310:1325	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	9	80	dep	synthesis	1299:1307	arg1	synthase					1339:1346	callose synthase	1331:1346	callose synthase	1331:1346	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	10	81	dep	pectins	1422:1428	arg1	mainly					1415:1420	mainly	1415:1420	mainly	1415:1420	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	7	82	theme	pollen	906:911	arg1	tubes					913:917	pollen tubes	906:917	pollen tubes	906:917	Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose.					
29659664	10	83	theme	cell	1395:1398	arg1	material					1405:1412	newly secreted cell wall material	1380:1412	newly secreted cell wall material (mainly pectins)	1380:1429	Furthermore, both callose and newly secreted cell wall material (mainly pectins) showed an altered distribution corresponding to the lack of oscillatory growth in pollen tubes.					
29659664	4	84	theme	pollen	519:524	arg1	tubes					526:530	Nicotiana tabacum pollen tubes	501:530	Nicotiana tabacum pollen tubes	501:530	Methods We analysed the effects of energy deficiency on the development of Nicotiana tabacum pollen tubes by replacing sucrose with glycerol in the growth medium.					
29659664	14	85	theme	pollen	1979:1984	arg1	growth					1991:1996	pollen tube growth	1979:1996	pollen tube growth	1979:1996	The evidence that different aspects of pollen tube growth are affected is an indication that pollen tubes adapt to metabolic stress.					
29659664	9	86	theme	enzymes	1270:1276	arg1	distribution					1250:1261	distribution	1250:1261	distribution	1250:1261	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	9	86	theme	enzymes	1270:1276	arg1	content					1238:1244	content	1238:1244	content	1238:1244	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	3	87	theme	pollen	351:356	arg1	growth					363:368	pollen tube growth	351:368	pollen tube growth	351:368	The lack of carbon sources can cause various problems during pollen tube growth, which in turn could affect the reproduction of plants.					
29659664	6	88	theme	growth	808:813	arg1	period					815:820	a specific growth period	797:820	a specific growth period	797:820	Key Results We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period.					
29659664	3	89	theme	plants	418:423	arg1	reproduction					402:413	the reproduction	398:413	the reproduction of plants	398:423	The lack of carbon sources can cause various problems during pollen tube growth, which in turn could affect the reproduction of plants.					
29659664	0	90	theme	tip	44:46	arg1	mechanism					55:63	the tip growth mechanism	40:63	the tip growth mechanism of tobacco pollen tubes	40:87	Depletion of sucrose induces changes in the tip growth mechanism of tobacco pollen tubes.					
29659664	9	91	theme	wall	1294:1297	arg1	synthesis					1299:1307	cell wall synthesis	1289:1307	cell wall synthesis (sucrose synthase and callose synthase)	1289:1347	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	8	92	theme	intracellular	1049:1061	arg1	pH					1063:1064	The intracellular pH	1045:1064	The intracellular pH	1045:1064	The intracellular pH and ROS levels also showed a different distribution in pollen tubes grown in sucrose-depleted media.					
29659664	9	93	dep	content	1238:1244	arg1	the					1234:1236	the	1234:1236	the	1234:1236	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	14	94	theme	growth	1991:1996	arg1	aspects					1968:1974	different aspects	1958:1974	different aspects of pollen tube growth	1958:1996	The evidence that different aspects of pollen tube growth are affected is an indication that pollen tubes adapt to metabolic stress.					
29659664	13	95	theme	cell	1918:1921	arg1	deposition					1928:1937	cell wall deposition	1918:1937	cell wall deposition	1918:1937	In our study, we found evidence that glycerol, a less energetic source for cell growth than sucrose, causes critical changes in cell wall deposition.					
29659664	12	96	theme	plant	1777:1781	arg1	cells					1783:1787	plant cells	1777:1787	plant cells	1777:1787	Conclusion Pollen tubes represent an ideal model system for studying metabolic pathways during the growth of plant cells.					
29659664	8	97	from	distribution	1105:1116	arg1	tubes					1128:1132	pollen tubes	1121:1132	pollen tubes grown in sucrose-depleted media	1121:1164	The intracellular pH and ROS levels also showed a different distribution in pollen tubes grown in sucrose-depleted media.					
29659664	11	98	theme	glycerol-based	1537:1550	arg1	media					1552:1556	glycerol-based media	1537:1556	glycerol-based media	1537:1556	Growth in glycerol-based media also temporarily affected the movement of generative cells and, in parallel, the deposition of callose plugs.					
29659664	6	99	theme	pollen	771:776	arg1	length					783:788	pollen tube length	771:788	pollen tube length	771:788	Key Results We found that the lack of sucrose affects pollen germination and pollen tube length during a specific growth period.					
29659664	12	100	theme	ideal	1705:1709	arg1	system					1717:1722	an ideal model system	1702:1722	an ideal model system for studying metabolic pathways during the growth of plant cells	1702:1787	Conclusion Pollen tubes represent an ideal model system for studying metabolic pathways during the growth of plant cells.					
29659664	9	101	located	observed	1185:1192	arg2	Changes					1167:1173	Changes	1167:1173	Changes	1167:1173	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	9	101	located	observed	1185:1192	arg1	distribution					1250:1261	distribution	1250:1261	distribution	1250:1261	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	9	101	located	observed	1185:1192	arg1	content					1238:1244	content	1238:1244	content	1238:1244	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	9	101	located	observed	1185:1192	arg1	level					1211:1215	the cell wall level	1197:1215	the cell wall level	1197:1215	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
29659664	7	102	theme	sugar	828:832	arg1	metabolism					834:843	sugar metabolism	828:843	sugar metabolism	828:843	Both sugar metabolism and ATP concentration were affected by sucrose shortage when pollen tubes were grown in glycerol-based media; this was related to decreases in the concentrations of glucose, fructose and UDP-glucose.					
29659664	9	103	theme	callose	1331:1337	arg1	synthase					1339:1346	callose synthase	1331:1346	callose synthase	1331:1346	Changes were also observed at the cell wall level, particularly in the content and distribution of two enzymes related to cell wall synthesis (sucrose synthase and callose synthase).					
30767229	0	0	theme	films	96:100	arg1	properties					41:50	physicochemical properties	25:50	physicochemical properties	25:50	Effect of lactoferrin on physicochemical properties and microstructure of pullulan-based edible films.					
30767229	0	0	theme	films	96:100	arg1	microstructure					56:69	microstructure	56:69	microstructure	56:69	Effect of lactoferrin on physicochemical properties and microstructure of pullulan-based edible films.					
30767229	5	1	theme	concentrations	630:643	arg1	presence					614:621	The presence	610:621	The presence of low concentrations of LF (< 0.03%)	610:659	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	2	2	dep	both	282:285	arg1	food					287:290	food	287:290	food	287:290	However, pure pullulan film is usually brittle and very hydrophilic, which limit its use in both food and pharmaceutical fields.					
30767229	12	3	theme	Chemical	1510:1517	arg1	Industry					1519:1526	Chemical Industry	1510:1526	Chemical Industry	1510:1526	© 2019 Society of Chemical Industry.					
30767229	0	4	theme	edible	89:94	arg1	films					96:100	pullulan-based edible films	74:100	pullulan-based edible films	74:100	Effect of lactoferrin on physicochemical properties and microstructure of pullulan-based edible films.					
30767229	9	5	theme	molecules	1238:1246	arg1	structure					1222:1230	the secondary structure	1208:1230	the secondary structure of LF molecules	1208:1246	Analysis from Fourier transform infrared spectroscopy indicated that the secondary structure of LF molecules was modified during the drying process.					
30767229	1	6	theme	edible	176:181	arg1	films					183:187	edible films	176:187	edible films	176:187	BACKGROUND Pullulan is a polysaccharide polymer commonly used to produce edible films.					
30767229	7	7	from	observation	987:997	arg1	particles					968:976	spherical particles	958:976	spherical particles from the observation of microscopy	958:1011	LF molecules aggregated with each other during the film-producing process, which presented as spherical particles from the observation of microscopy.					
30767229	0	8	from	Effect	0:5	arg1	properties					41:50	physicochemical properties	25:50	physicochemical properties	25:50	Effect of lactoferrin on physicochemical properties and microstructure of pullulan-based edible films.					
30767229	0	8	from	Effect	0:5	arg1	microstructure					56:69	microstructure	56:69	microstructure	56:69	Effect of lactoferrin on physicochemical properties and microstructure of pullulan-based edible films.					
30767229	5	9	theme	LF	648:649	arg1	concentrations					630:643	low concentrations	626:643	low concentrations of LF (< 0.03%)	626:659	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	10	from	break	733:737	arg1	strength					701:708	tensile strength	693:708	tensile strength (TS)	693:713	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	10	from	break	733:737	arg1	EAB					740:742	EAB	740:742	EAB	740:742	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	10	from	break	733:737	arg1	TS					711:712	TS	711:712	TS	711:712	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	10	from	break	733:737	arg1	elongation					719:728	elongation	719:728	elongation at break	719:737	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	11	theme	tensile	693:699	arg1	strength					701:708	tensile strength	693:708	tensile strength (TS)	693:713	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	11	theme	tensile	693:699	arg1	EAB					740:742	EAB	740:742	EAB	740:742	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	11	theme	tensile	693:699	arg1	TS					711:712	TS	711:712	TS	711:712	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	11	12	used	used	1455:1458	arg2	film					1431:1434	The produced composite film	1408:1434	The produced composite film	1408:1434	The produced composite film can be potentially used for food and medical packaging.					
30767229	10	13	theme	film	1358:1361	arg1	hydrophobicity					1331:1344	the hydrophobicity	1327:1344	the hydrophobicity of pullulan film	1327:1361	CONCLUSIONS It is possible to increase the hydrophobicity of pullulan film while maintaining its mechanical properties.					
30767229	3	14	theme	research	341:348	arg1	objective					323:331	The objective	319:331	The objective of this research	319:348	The objective of this research is to improve the structural and mechanical properties of pullulan film by incorporating globular protein lactoferrin (LF).					
30767229	9	15	theme	drying	1272:1277	arg1	process					1279:1285	the drying process	1268:1285	the drying process	1268:1285	Analysis from Fourier transform infrared spectroscopy indicated that the secondary structure of LF molecules was modified during the drying process.					
30767229	9	16	theme	infrared	1171:1178	arg1	spectroscopy					1180:1191	infrared spectroscopy	1171:1191	infrared spectroscopy	1171:1191	Analysis from Fourier transform infrared spectroscopy indicated that the secondary structure of LF molecules was modified during the drying process.					
30767229	11	17	theme	produced	1412:1419	arg1	film					1431:1434	The produced composite film	1408:1434	The produced composite film	1408:1434	The produced composite film can be potentially used for food and medical packaging.					
30767229	3	18	theme	globular	439:446	arg1	lactoferrin					456:466	globular protein lactoferrin	439:466	globular protein lactoferrin (LF)	439:471	The objective of this research is to improve the structural and mechanical properties of pullulan film by incorporating globular protein lactoferrin (LF).					
30767229	3	18	theme	globular	439:446	arg1	LF					469:470	LF	469:470	LF	469:470	The objective of this research is to improve the structural and mechanical properties of pullulan film by incorporating globular protein lactoferrin (LF).					
30767229	3	19	theme	structural	368:377	arg1	properties					394:403	the structural and mechanical properties	364:403	the structural and mechanical properties of pullulan film	364:420	The objective of this research is to improve the structural and mechanical properties of pullulan film by incorporating globular protein lactoferrin (LF).					
30767229	5	20	contain	has	661:663	arg2	influence					680:688	no significant influence	665:688	no significant influence	665:688	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	20	contain	has	661:663	arg1	presence					614:621	The presence	610:621	The presence of low concentrations of LF (< 0.03%)	610:659	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	7	21	theme	LF	864:865	arg1	molecules					867:875	LF molecules	864:875	LF molecules	864:875	LF molecules aggregated with each other during the film-producing process, which presented as spherical particles from the observation of microscopy.					
30767229	11	22	theme	composite	1421:1429	arg1	film					1431:1434	The produced composite film	1408:1434	The produced composite film	1408:1434	The produced composite film can be potentially used for food and medical packaging.					
30767229	3	23	theme	film	417:420	arg1	properties					394:403	the structural and mechanical properties	364:403	the structural and mechanical properties of pullulan film	364:420	The objective of this research is to improve the structural and mechanical properties of pullulan film by incorporating globular protein lactoferrin (LF).					
30767229	5	24	theme	low	626:628	arg1	concentrations					630:643	low concentrations	626:643	low concentrations of LF (< 0.03%)	626:659	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	1	25	theme	BACKGROUND	103:112	arg1	polymer					143:149	a polysaccharide polymer	126:149	a polysaccharide polymer commonly used to produce edible films	126:187	BACKGROUND Pullulan is a polysaccharide polymer commonly used to produce edible films.					
30767229	1	25	theme	BACKGROUND	103:112	arg1	Pullulan					114:121	BACKGROUND Pullulan	103:121	BACKGROUND Pullulan	103:121	BACKGROUND Pullulan is a polysaccharide polymer commonly used to produce edible films.					
30767229	5	26	theme	pullulan	748:755	arg1	film					757:760	pullulan film	748:760	pullulan film	748:760	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	4	27	dep	RESULTS	474:480	arg1	decreased					547:555	decreased	547:555	decreased the water vapor permeability (WVP) of pullulan film	547:607	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	4	27	dep	RESULTS	474:480	arg1	increased					506:514	increased	506:514	increased the surface hydrophobicity	506:541	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	10	28	theme	mechanical	1385:1394	arg1	properties					1396:1405	its mechanical properties	1381:1405	its mechanical properties	1381:1405	CONCLUSIONS It is possible to increase the hydrophobicity of pullulan film while maintaining its mechanical properties.					
30767229	11	29	theme	medical	1473:1479	arg1	packaging					1481:1489	medical packaging	1473:1489	medical packaging	1473:1489	The produced composite film can be potentially used for food and medical packaging.					
30767229	2	30	dep	brittle	229:235	arg1	hydrophilic					246:256	hydrophilic	246:256	hydrophilic	246:256	However, pure pullulan film is usually brittle and very hydrophilic, which limit its use in both food and pharmaceutical fields.					
30767229	0	31	theme	lactoferrin	10:20	arg1	Effect					0:5	Effect	0:5	Effect of lactoferrin on physicochemical properties and microstructure of pullulan-based edible films.	0:101	Effect of lactoferrin on physicochemical properties and microstructure of pullulan-based edible films.					
30767229	4	32	theme	vapor	567:571	arg1	WVP					587:589	WVP	587:589	WVP	587:589	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	4	32	theme	vapor	567:571	arg1	permeability					573:584	the water vapor permeability	557:584	the water vapor permeability (WVP) of pullulan film	557:607	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	7	33	theme	microscopy	1002:1011	arg1	observation					987:997	the observation	983:997	the observation of microscopy	983:1011	LF molecules aggregated with each other during the film-producing process, which presented as spherical particles from the observation of microscopy.					
30767229	6	34	theme	concentration	795:807	arg1	increase					780:787	further increase	772:787	further increase of LF concentration to levels > 0.03%	772:825	However, further increase of LF concentration to levels > 0.03% resulted in a film-weakening effect.					
30767229	4	35	theme	water	561:565	arg1	WVP					587:589	WVP	587:589	WVP	587:589	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	4	35	theme	water	561:565	arg1	permeability					573:584	the water vapor permeability	557:584	the water vapor permeability (WVP) of pullulan film	557:607	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	5	36	theme	significant	668:678	arg1	influence					680:688	no significant influence	665:688	no significant influence	665:688	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	6	37	theme	LF	792:793	arg1	concentration					795:807	LF concentration	792:807	LF concentration	792:807	However, further increase of LF concentration to levels > 0.03% resulted in a film-weakening effect.					
30767229	3	38	theme	protein	448:454	arg1	lactoferrin					456:466	globular protein lactoferrin	439:466	globular protein lactoferrin (LF)	439:471	The objective of this research is to improve the structural and mechanical properties of pullulan film by incorporating globular protein lactoferrin (LF).					
30767229	3	38	theme	protein	448:454	arg1	LF					469:470	LF	469:470	LF	469:470	The objective of this research is to improve the structural and mechanical properties of pullulan film by incorporating globular protein lactoferrin (LF).					
30767229	0	39	theme	physicochemical	25:39	arg1	properties					41:50	physicochemical properties	25:50	physicochemical properties	25:50	Effect of lactoferrin on physicochemical properties and microstructure of pullulan-based edible films.					
30767229	4	40	theme	surface	520:526	arg1	hydrophobicity					528:541	the surface hydrophobicity	516:541	the surface hydrophobicity	516:541	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	3	41	theme	pullulan	408:415	arg1	film					417:420	pullulan film	408:420	pullulan film	408:420	The objective of this research is to improve the structural and mechanical properties of pullulan film by incorporating globular protein lactoferrin (LF).					
30767229	9	42	theme	secondary	1212:1220	arg1	structure					1222:1230	the secondary structure	1208:1230	the secondary structure of LF molecules	1208:1246	Analysis from Fourier transform infrared spectroscopy indicated that the secondary structure of LF molecules was modified during the drying process.					
30767229	3	43	theme	mechanical	383:392	arg1	properties					394:403	the structural and mechanical properties	364:403	the structural and mechanical properties of pullulan film	364:420	The objective of this research is to improve the structural and mechanical properties of pullulan film by incorporating globular protein lactoferrin (LF).					
30767229	5	44	theme	<	652:652	arg1	%					658:658	< 0.03%	652:658	< 0.03%	652:658	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	44	theme	<	652:652	arg1	LF					648:649	LF	648:649	LF (< 0.03%)	648:659	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	10	45	theme	pullulan	1349:1356	arg1	film					1358:1361	pullulan film	1349:1361	pullulan film	1349:1361	CONCLUSIONS It is possible to increase the hydrophobicity of pullulan film while maintaining its mechanical properties.					
30767229	4	46	theme	LF	503:504	arg1	incorporation					486:498	The incorporation	482:498	The incorporation of LF	482:504	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	6	47	theme	further	772:778	arg1	increase					780:787	further increase	772:787	further increase of LF concentration to levels > 0.03%	772:825	However, further increase of LF concentration to levels > 0.03% resulted in a film-weakening effect.					
30767229	1	48	theme	polysaccharide	128:141	arg1	polymer					143:149	a polysaccharide polymer	126:149	a polysaccharide polymer commonly used to produce edible films	126:187	BACKGROUND Pullulan is a polysaccharide polymer commonly used to produce edible films.					
30767229	1	48	theme	polysaccharide	128:141	arg1	Pullulan					114:121	BACKGROUND Pullulan	103:121	BACKGROUND Pullulan	103:121	BACKGROUND Pullulan is a polysaccharide polymer commonly used to produce edible films.					
30767229	2	49	from	use	275:277	arg1	fields					311:316	both food and pharmaceutical fields	282:316	both food and pharmaceutical fields	282:316	However, pure pullulan film is usually brittle and very hydrophilic, which limit its use in both food and pharmaceutical fields.					
30767229	6	50	theme	film-weakening	841:854	arg1	effect					856:861	a film-weakening effect	839:861	a film-weakening effect	839:861	However, further increase of LF concentration to levels > 0.03% resulted in a film-weakening effect.					
30767229	8	51	theme	pullulan	1067:1074	arg1	matrix					1076:1081	the pullulan matrix	1063:1081	the pullulan matrix	1063:1081	LF aggregates dispersed homogeneously throughout the pullulan matrix and their size increased with increasing concentration.					
30767229	9	52	theme	LF	1235:1236	arg1	molecules					1238:1246	LF molecules	1235:1246	LF molecules	1235:1246	Analysis from Fourier transform infrared spectroscopy indicated that the secondary structure of LF molecules was modified during the drying process.					
30767229	10	53	dep	CONCLUSIONS	1288:1298	arg1	possible					1306:1313	possible	1306:1313	possible	1306:1313	CONCLUSIONS It is possible to increase the hydrophobicity of pullulan film while maintaining its mechanical properties.					
30767229	2	54	dep	fields	311:316	arg1	both					282:285	both	282:285	both	282:285	However, pure pullulan film is usually brittle and very hydrophilic, which limit its use in both food and pharmaceutical fields.					
30767229	2	54	dep	fields	311:316	arg1	pharmaceutical					296:309	pharmaceutical	296:309	pharmaceutical	296:309	However, pure pullulan film is usually brittle and very hydrophilic, which limit its use in both food and pharmaceutical fields.					
30767229	9	55	dep	Fourier	1153:1159	arg1	transform					1161:1169	transform	1161:1169	transform infrared spectroscopy	1161:1191	Analysis from Fourier transform infrared spectroscopy indicated that the secondary structure of LF molecules was modified during the drying process.					
30767229	4	56	theme	pullulan	595:602	arg1	film					604:607	pullulan film	595:607	pullulan film	595:607	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	7	57	theme	spherical	958:966	arg1	particles					968:976	spherical particles	958:976	spherical particles from the observation of microscopy	958:1011	LF molecules aggregated with each other during the film-producing process, which presented as spherical particles from the observation of microscopy.					
30767229	2	58	theme	pullulan	204:211	arg1	film					213:216	pure pullulan film	199:216	pure pullulan film	199:216	However, pure pullulan film is usually brittle and very hydrophilic, which limit its use in both food and pharmaceutical fields.					
30767229	8	59	dep	aggregates	1017:1026	arg1	dispersed					1028:1036	dispersed	1028:1036	aggregates dispersed homogeneously throughout the pullulan matrix	1017:1081	LF aggregates dispersed homogeneously throughout the pullulan matrix and their size increased with increasing concentration.					
30767229	0	60	theme	pullulan-based	74:87	arg1	films					96:100	pullulan-based edible films	74:100	pullulan-based edible films	74:100	Effect of lactoferrin on physicochemical properties and microstructure of pullulan-based edible films.					
30767229	2	61	theme	pure	199:202	arg1	film					213:216	pure pullulan film	199:216	pure pullulan film	199:216	However, pure pullulan film is usually brittle and very hydrophilic, which limit its use in both food and pharmaceutical fields.					
30767229	7	62	theme	film-producing	915:928	arg1	process					930:936	the film-producing process	911:936	the film-producing process	911:936	LF molecules aggregated with each other during the film-producing process, which presented as spherical particles from the observation of microscopy.					
30767229	5	63	theme	film	757:760	arg1	strength					701:708	tensile strength	693:708	tensile strength (TS)	693:713	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	63	theme	film	757:760	arg1	EAB					740:742	EAB	740:742	EAB	740:742	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	63	theme	film	757:760	arg1	TS					711:712	TS	711:712	TS	711:712	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	5	63	theme	film	757:760	arg1	elongation					719:728	elongation	719:728	elongation at break	719:737	The presence of low concentrations of LF (< 0.03%) has no significant influence on tensile strength (TS) and elongation at break (EAB) of pullulan film.					
30767229	9	64	from	Fourier	1153:1159	arg1	Analysis					1139:1146	Analysis	1139:1146	Analysis from Fourier transform infrared spectroscopy	1139:1191	Analysis from Fourier transform infrared spectroscopy indicated that the secondary structure of LF molecules was modified during the drying process.					
30767229	4	65	theme	film	604:607	arg1	WVP					587:589	WVP	587:589	WVP	587:589	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
30767229	4	65	theme	film	604:607	arg1	permeability					573:584	the water vapor permeability	557:584	the water vapor permeability (WVP) of pullulan film	557:607	RESULTS The incorporation of LF increased the surface hydrophobicity and decreased the water vapor permeability (WVP) of pullulan film.					
29783150	6	0	theme	inducible	954:962	arg1	iNOS					987:990	iNOS	987:990	iNOS	987:990	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	6	0	theme	inducible	954:962	arg1	synthase					977:984	inducible nitric oxide synthase	954:984	inducible nitric oxide synthase (iNOS)	954:991	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	7	1	theme	MC3T3-E1	1024:1031	arg1	osteoblasts					1033:1043	MC3T3-E1 osteoblasts	1024:1043	MC3T3-E1 osteoblasts	1024:1043	Osteogenic differentiation of MC3T3-E1 osteoblasts on DEX-loaded coatings was investigated by osteogenic gene expression and mineralization.					
29783150	1	2	theme	bioactive	196:204	arg1	coatings					224:231	bioactive and biodegradable coatings	196:231	bioactive and biodegradable coatings	196:231	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	4	3	theme	stable	557:562	arg1	coatings					613:620	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings	541:620	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings	541:620	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings were formed by electrophoretic deposition (EPD).					
29783150	7	4	theme	Osteogenic	994:1003	arg1	differentiation					1005:1019	Osteogenic differentiation	994:1019	Osteogenic differentiation of MC3T3-E1 osteoblasts on DEX-loaded coatings	994:1066	Osteogenic differentiation of MC3T3-E1 osteoblasts on DEX-loaded coatings was investigated by osteogenic gene expression and mineralization.					
29783150	7	5	from	differentiation	1005:1019	arg1	coatings					1059:1066	DEX-loaded coatings	1048:1066	DEX-loaded coatings	1048:1066	Osteogenic differentiation of MC3T3-E1 osteoblasts on DEX-loaded coatings was investigated by osteogenic gene expression and mineralization.					
29783150	5	6	theme	liquid	776:781	arg1	HPLC					799:802	HPLC	799:802	HPLC	799:802	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	5	6	theme	liquid	776:781	arg1	chromatography					783:796	high performance liquid chromatography	759:796	high performance liquid chromatography (HPLC)	759:803	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	5	7	theme	in	714:715	arg1	release					723:729	in vitro release	714:729	in vitro release of DEX	714:736	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	6	8	theme	RAW	872:874	arg1	cells					882:886	macrophage RAW 264.7 cells	861:886	macrophage RAW 264.7 cells	861:886	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	2	9	theme	chitosan/gelatin	329:344	arg1	coating					374:380	chitosan/gelatin nanospheres (GNs) composite coating	329:380	chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX)	329:420	The objective of this study was to prepare chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX).					
29783150	3	10	with	GNs	423:425	arg1	charge					478:483	negative surface charge	461:483	negative surface charge	461:483	GNs with narrow size distribution and negative surface charge were firstly prepared by a two-step desolvation method.					
29783150	3	10	with	GNs	423:425	arg1	distribution					444:455	narrow size distribution	432:455	narrow size distribution	432:455	GNs with narrow size distribution and negative surface charge were firstly prepared by a two-step desolvation method.					
29783150	6	11	theme	macrophage	861:870	arg1	cells					882:886	macrophage RAW 264.7 cells	861:886	macrophage RAW 264.7 cells	861:886	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	1	12	theme	metallic	173:180	arg1	implants					182:189	metallic implants	173:189	metallic implants	173:189	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	1	13	theme	Surface	149:155	arg1	approach					254:261	a promising approach	242:261	a promising approach for bone regeneration	242:283	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	1	13	theme	Surface	149:155	arg1	modification					157:168	Surface modification	149:168	Surface modification of metallic implants with bioactive and biodegradable coatings	149:231	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	5	14	theme	Drug	671:674	arg1	loading					676:682	Drug loading	671:682	Drug loading	671:682	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	6	15	from	effect	828:833	arg1	cells					882:886	macrophage RAW 264.7 cells	861:886	macrophage RAW 264.7 cells	861:886	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	1	16	theme	biodegradable	210:222	arg1	coatings					224:231	bioactive and biodegradable coatings	196:231	bioactive and biodegradable coatings	196:231	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	4	17	theme	electrophoretic	637:651	arg1	EPD					665:667	EPD	665:667	EPD	665:667	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings were formed by electrophoretic deposition (EPD).					
29783150	4	17	theme	electrophoretic	637:651	arg1	deposition					653:662	electrophoretic deposition	637:662	electrophoretic deposition (EPD)	637:668	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings were formed by electrophoretic deposition (EPD).					
29783150	6	18	theme	factor	937:942	arg1	secretion					908:916	the secretion	904:916	the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS)	904:991	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	6	19	theme	coatings	849:856	arg1	effect					828:833	The anti-inflammatory effect	806:833	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells	806:886	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	8	20	theme	composite	1154:1162	arg1	coating					1164:1170	composite coating	1154:1170	composite coating	1154:1170	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
29783150	8	21	theme	osteogenic	1294:1303	arg1	differentiation					1305:1319	osteogenic differentiation	1294:1319	osteogenic differentiation	1294:1319	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
29783150	5	22	dep	in	714:715	arg1	vitro					717:721	vitro	717:721	vitro	717:721	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	6	23	theme	necrosis	928:935	arg1	TNF					945:947	TNF	945:947	TNF	945:947	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	6	23	theme	necrosis	928:935	arg1	factor					937:942	tumour necrosis factor	921:942	tumour necrosis factor (TNF)	921:948	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	6	24	theme	DEX-loaded	838:847	arg1	coatings					849:856	DEX-loaded coatings	838:856	DEX-loaded coatings	838:856	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	0	25	from	coating	80:86	arg1	osteogenesis					135:146	osteogenesis	135:146	osteogenesis	135:146	Electrophoretic deposition of dexamethasone-loaded gelatin nanospheres/chitosan coating and its dual function in anti-inflammation and osteogenesis.					
29783150	0	25	from	coating	80:86	arg1	anti-inflammation					113:129	anti-inflammation	113:129	anti-inflammation	113:129	Electrophoretic deposition of dexamethasone-loaded gelatin nanospheres/chitosan coating and its dual function in anti-inflammation and osteogenesis.					
29783150	1	26	mod	modification	157:168	arg3	Surface					149:155	Surface modification	149:168	Surface modification of metallic implants with bioactive and biodegradable coatings	149:231	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	1	26	mod	modification	157:168	arg1	implants					182:189	metallic implants	173:189	metallic implants	173:189	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	0	27	theme	Electrophoretic	0:14	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of dexamethasone-loaded gelatin	0:57	Electrophoretic deposition of dexamethasone-loaded gelatin nanospheres/chitosan coating and its dual function in anti-inflammation and osteogenesis.					
29783150	4	28	theme	composite	603:611	arg1	coatings					613:620	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings	541:620	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings	541:620	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings were formed by electrophoretic deposition (EPD).					
29783150	1	29	with	modification	157:168	arg1	coatings					224:231	bioactive and biodegradable coatings	196:231	bioactive and biodegradable coatings	196:231	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	2	30	theme	GNs	359:361	arg1	coating					374:380	chitosan/gelatin nanospheres (GNs) composite coating	329:380	chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX)	329:420	The objective of this study was to prepare chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX).					
29783150	8	31	theme	sustained	1328:1336	arg1	period					1346:1351	the sustained release period	1324:1351	the sustained release period	1324:1351	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
29783150	3	32	theme	negative	461:468	arg1	charge					478:483	negative surface charge	461:483	negative surface charge	461:483	GNs with narrow size distribution and negative surface charge were firstly prepared by a two-step desolvation method.					
29783150	0	33	theme	dexamethasone-loaded	30:49	arg1	gelatin					51:57	dexamethasone-loaded gelatin	30:57	dexamethasone-loaded gelatin	30:57	Electrophoretic deposition of dexamethasone-loaded gelatin nanospheres/chitosan coating and its dual function in anti-inflammation and osteogenesis.					
29783150	8	34	dep	GNs/CTS	1146:1152	arg1	showed					1172:1177	showed	1172:1177	showed a two-stage release pattern	1172:1205	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
29783150	8	35	theme	release	1338:1344	arg1	period					1346:1351	the sustained release period	1324:1351	the sustained release period	1324:1351	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
29783150	8	36	theme	release	1261:1267	arg1	period					1269:1274	the burst release period	1251:1274	the burst release period	1251:1274	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
29783150	6	37	theme	tumour	921:926	arg1	TNF					945:947	TNF	945:947	TNF	945:947	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	6	37	theme	tumour	921:926	arg1	factor					937:942	tumour necrosis factor	921:942	tumour necrosis factor (TNF)	921:948	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	2	38	theme	nanospheres	346:356	arg1	coating					374:380	chitosan/gelatin nanospheres (GNs) composite coating	329:380	chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX)	329:420	The objective of this study was to prepare chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX).					
29783150	1	39	theme	promising	244:252	arg1	approach					254:261	a promising approach	242:261	a promising approach for bone regeneration	242:283	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	1	39	theme	promising	244:252	arg1	modification					157:168	Surface modification	149:168	Surface modification of metallic implants with bioactive and biodegradable coatings	149:231	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	3	40	theme	size	439:442	arg1	distribution					444:455	narrow size distribution	432:455	narrow size distribution	432:455	GNs with narrow size distribution and negative surface charge were firstly prepared by a two-step desolvation method.					
29783150	5	41	theme	high	759:762	arg1	HPLC					799:802	HPLC	799:802	HPLC	799:802	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	5	41	theme	high	759:762	arg1	chromatography					783:796	high performance liquid chromatography	759:796	high performance liquid chromatography (HPLC)	759:803	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	0	42	from	function	101:108	arg1	osteogenesis					135:146	osteogenesis	135:146	osteogenesis	135:146	Electrophoretic deposition of dexamethasone-loaded gelatin nanospheres/chitosan coating and its dual function in anti-inflammation and osteogenesis.					
29783150	0	42	from	function	101:108	arg1	anti-inflammation					113:129	anti-inflammation	113:129	anti-inflammation	113:129	Electrophoretic deposition of dexamethasone-loaded gelatin nanospheres/chitosan coating and its dual function in anti-inflammation and osteogenesis.					
29783150	9	43	theme	bone	1445:1448	arg1	regeneration					1450:1461	bone regeneration	1445:1461	bone regeneration	1445:1461	This study might offer a feasible method for modifying the surface of metallic implants in bone regeneration.					
29783150	0	44	theme	gelatin	51:57	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of dexamethasone-loaded gelatin	0:57	Electrophoretic deposition of dexamethasone-loaded gelatin nanospheres/chitosan coating and its dual function in anti-inflammation and osteogenesis.					
29783150	9	45	theme	metallic	1424:1431	arg1	implants					1433:1440	metallic implants	1424:1440	metallic implants	1424:1440	This study might offer a feasible method for modifying the surface of metallic implants in bone regeneration.					
29783150	5	46	theme	performance	764:774	arg1	HPLC					799:802	HPLC	799:802	HPLC	799:802	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	5	46	theme	performance	764:774	arg1	chromatography					783:796	high performance liquid chromatography	759:796	high performance liquid chromatography (HPLC)	759:803	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	6	47	theme	anti-inflammatory	810:826	arg1	effect					828:833	The anti-inflammatory effect	806:833	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells	806:886	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	8	48	theme	burst	1255:1259	arg1	period					1269:1274	the burst release period	1251:1274	the burst release period	1251:1274	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
29783150	9	49	theme	implants	1433:1440	arg1	surface					1413:1419	the surface	1409:1419	the surface of metallic implants in bone regeneration	1409:1461	This study might offer a feasible method for modifying the surface of metallic implants in bone regeneration.					
29783150	7	50	theme	gene	1099:1102	arg1	expression					1104:1113	osteogenic gene expression	1088:1113	osteogenic gene expression	1088:1113	Osteogenic differentiation of MC3T3-E1 osteoblasts on DEX-loaded coatings was investigated by osteogenic gene expression and mineralization.					
29783150	3	51	theme	surface	470:476	arg1	charge					478:483	negative surface charge	461:483	negative surface charge	461:483	GNs with narrow size distribution and negative surface charge were firstly prepared by a two-step desolvation method.					
29783150	8	52	theme	two-stage	1181:1189	arg1	pattern					1199:1205	a two-stage release pattern	1179:1205	a two-stage release pattern	1179:1205	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
29783150	3	53	theme	desolvation	521:531	arg1	method					533:538	a two-step desolvation method	510:538	a two-step desolvation method	510:538	GNs with narrow size distribution and negative surface charge were firstly prepared by a two-step desolvation method.					
29783150	3	54	theme	narrow	432:437	arg1	distribution					444:455	narrow size distribution	432:455	narrow size distribution	432:455	GNs with narrow size distribution and negative surface charge were firstly prepared by a two-step desolvation method.					
29783150	6	55	theme	synthase	977:984	arg1	secretion					908:916	the secretion	904:916	the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS)	904:991	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	6	56	theme	nitric	964:969	arg1	iNOS					987:990	iNOS	987:990	iNOS	987:990	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	6	56	theme	nitric	964:969	arg1	synthase					977:984	inducible nitric oxide synthase	954:984	inducible nitric oxide synthase (iNOS)	954:991	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	7	57	theme	DEX-loaded	1048:1057	arg1	coatings					1059:1066	DEX-loaded coatings	1048:1066	DEX-loaded coatings	1048:1066	Osteogenic differentiation of MC3T3-E1 osteoblasts on DEX-loaded coatings was investigated by osteogenic gene expression and mineralization.					
29783150	5	58	theme	encapsulation	685:697	arg1	efficiency					699:708	encapsulation efficiency	685:708	encapsulation efficiency	685:708	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	6	59	theme	oxide	971:975	arg1	iNOS					987:990	iNOS	987:990	iNOS	987:990	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	6	59	theme	oxide	971:975	arg1	synthase					977:984	inducible nitric oxide synthase	954:984	inducible nitric oxide synthase (iNOS)	954:991	The anti-inflammatory effect of DEX-loaded coatings on macrophage RAW 264.7 cells was assessed by the secretion of tumour necrosis factor (TNF) and inducible nitric oxide synthase (iNOS).					
29783150	5	60	theme	DEX	734:736	arg1	release					723:729	in vitro release	714:729	in vitro release of DEX	714:736	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	5	60	theme	DEX	734:736	arg1	loading					676:682	Drug loading	671:682	Drug loading	671:682	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	5	60	theme	DEX	734:736	arg1	efficiency					699:708	encapsulation efficiency	685:708	encapsulation efficiency	685:708	Drug loading, encapsulation efficiency and in vitro release of DEX were estimated using high performance liquid chromatography (HPLC).					
29783150	2	61	theme	study	308:312	arg1	objective					290:298	The objective	286:298	The objective of this study	286:312	The objective of this study was to prepare chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX).					
29783150	9	62	theme	feasible	1379:1386	arg1	method					1388:1393	a feasible method	1377:1393	a feasible method for modifying the surface of metallic implants in bone regeneration	1377:1461	This study might offer a feasible method for modifying the surface of metallic implants in bone regeneration.					
29783150	1	63	theme	bone	267:270	arg1	regeneration					272:283	bone regeneration	267:283	bone regeneration	267:283	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	7	64	theme	osteogenic	1088:1097	arg1	expression					1104:1113	osteogenic gene expression	1088:1113	osteogenic gene expression	1088:1113	Osteogenic differentiation of MC3T3-E1 osteoblasts on DEX-loaded coatings was investigated by osteogenic gene expression and mineralization.					
29783150	7	65	theme	osteoblasts	1033:1043	arg1	differentiation					1005:1019	Osteogenic differentiation	994:1019	Osteogenic differentiation of MC3T3-E1 osteoblasts on DEX-loaded coatings	994:1066	Osteogenic differentiation of MC3T3-E1 osteoblasts on DEX-loaded coatings was investigated by osteogenic gene expression and mineralization.					
29783150	2	66	theme	composite	364:372	arg1	coating					374:380	chitosan/gelatin nanospheres (GNs) composite coating	329:380	chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX)	329:420	The objective of this study was to prepare chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX).					
29783150	0	67	theme	dual	96:99	arg1	function					101:108	its dual function	92:108	its dual function in anti-inflammation and osteogenesis	92:146	Electrophoretic deposition of dexamethasone-loaded gelatin nanospheres/chitosan coating and its dual function in anti-inflammation and osteogenesis.					
29783150	9	68	from	surface	1413:1419	arg1	regeneration					1450:1461	bone regeneration	1445:1461	bone regeneration	1445:1461	This study might offer a feasible method for modifying the surface of metallic implants in bone regeneration.					
29783150	1	69	theme	implants	182:189	arg1	approach					254:261	a promising approach	242:261	a promising approach for bone regeneration	242:283	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	1	69	theme	implants	182:189	arg1	modification					157:168	Surface modification	149:168	Surface modification of metallic implants with bioactive and biodegradable coatings	149:231	Surface modification of metallic implants with bioactive and biodegradable coatings could be a promising approach for bone regeneration.					
29783150	3	70	theme	two-step	512:519	arg1	method					533:538	a two-step desolvation method	510:538	a two-step desolvation method	510:538	GNs with narrow size distribution and negative surface charge were firstly prepared by a two-step desolvation method.					
29783150	8	71	theme	release	1191:1197	arg1	pattern					1199:1205	a two-stage release pattern	1179:1205	a two-stage release pattern	1179:1205	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
29783150	4	72	theme	Homogeneous	541:551	arg1	coatings					613:620	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings	541:620	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings	541:620	Homogeneous and stable gelatin nanospheres/chitosan (GNs/CTS) composite coatings were formed by electrophoretic deposition (EPD).					
29783150	2	73	theme	dexamethasone	402:414	arg1	delivery					390:397	the delivery	386:397	the delivery of dexamethasone (DEX)	386:420	The objective of this study was to prepare chitosan/gelatin nanospheres (GNs) composite coating for the delivery of dexamethasone (DEX).					
29783150	8	74	from	DEX	1139:1141	arg1	GNs/CTS					1146:1152	GNs/CTS	1146:1152	GNs/CTS composite coating showed a two-stage release pattern	1146:1205	The DEX in GNs/CTS composite coating showed a two-stage release pattern could not only suppress inflammation during the burst release period, but also promote osteogenic differentiation in the sustained release period.					
31995891	9	0	theme	Further	1613:1619	arg1	results					1621:1627	Further results	1613:1627	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	1613:1687	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	8	1	theme	YE	1439:1440	arg1	higher					1489:1494	higher	1489:1494	higher	1489:1494	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	1	theme	YE	1439:1440	arg1	FRR					1385:1387	FRR	1385:1387	FRR	1385:1387	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	1	theme	YE	1439:1440	arg1	rate					1379:1382	a flux recovery rate	1363:1382	a flux recovery rate (FRR)	1363:1388	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	5	2	dep	MWCNTs	903:908	arg1	headed					910:915	headed	910:915	MWCNTs headed out of the PES-Ni@MWCNTs membrane surface	903:957	The morphology measurements confirmed that the Ni@MWCNTs headed out of the PES-Ni@MWCNTs membrane surface.					
31995891	6	3	theme	membrane	1107:1114	arg1	flux					1086:1089	threefold flux	1076:1089	threefold flux of the pristine membrane	1076:1114	Because the MWCNTs played a role of free channels for water molecules, the composite membrane water flux reached to threefold flux of the pristine membrane.					
31995891	8	4	theme	HA	1446:1447	arg1	higher					1489:1494	higher	1489:1494	higher	1489:1494	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	4	theme	HA	1446:1447	arg1	FRR					1385:1387	FRR	1385:1387	FRR	1385:1387	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	4	theme	HA	1446:1447	arg1	rate					1379:1382	a flux recovery rate	1363:1382	a flux recovery rate (FRR)	1363:1388	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	1	5	theme	composite	264:272	arg1	membranes					274:282	polymer-MWCNTs composite membranes	249:282	polymer-MWCNTs composite membranes	249:282	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	7	6	theme	HA	1288:1289	arg1	solutions					1291:1299	HA solutions	1288:1299	HA solutions	1288:1299	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	6	7	theme	water	1014:1018	arg1	molecules					1020:1028	water molecules	1014:1028	water molecules	1014:1028	Because the MWCNTs played a role of free channels for water molecules, the composite membrane water flux reached to threefold flux of the pristine membrane.					
31995891	9	8	theme	Derjaguin-Landau-Verwey-Overbeek	1641:1672	arg1	theory					1682:1687	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	1632:1687	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	1632:1687	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	3	9	theme	in-situ	603:609	arg1	reaction					621:628	in-situ reduction reaction	603:628	in-situ reduction reaction	603:628	In this study, the Ni@MWCNTs was generated by immersing MWCNTs into Ni2+ solution where in-situ reduction reaction was launched by the adsorbed Ag+ on MWCNTs.					
31995891	7	10	theme	YE	1281:1282	arg1	cycles					1252:1257	all the three alternative filtration cycles	1215:1257	all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions	1215:1299	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	4	11	theme	@	729:729	arg1	MWCNTs					730:735	the Ni@MWCNTs	723:735	the Ni@MWCNTs	723:735	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	6	12	theme	channels	1001:1008	arg1	role					988:991	a role	986:991	a role of free channels for water molecules	986:1028	Because the MWCNTs played a role of free channels for water molecules, the composite membrane water flux reached to threefold flux of the pristine membrane.					
31995891	9	13	theme	XDLVO	1675:1679	arg1	theory					1682:1687	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	1632:1687	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	1632:1687	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	9	14	contain	possessed	1726:1734	arg1	membrane					1717:1724	the modified membrane	1704:1724	the modified membrane	1704:1724	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	9	14	contain	possessed	1726:1734	arg2	energies					1761:1768	advantageous interaction energies	1736:1768	advantageous interaction energies	1736:1768	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	7	15	theme	filtration	1241:1250	arg1	cycles					1252:1257	all the three alternative filtration cycles	1215:1257	all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions	1215:1299	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	9	16	theme	theory	1682:1687	arg1	results					1621:1627	Further results	1613:1627	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	1613:1687	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	2	17	theme	few	294:296	arg1	reports					298:304	few reports	294:304	few reports	294:304	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	4	18	theme	external	793:800	arg1	field					811:815	an external magnetic field	790:815	an external magnetic field during the phase inversion process	790:850	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	6	19	theme	threefold	1076:1084	arg1	flux					1086:1089	threefold flux	1076:1089	threefold flux of the pristine membrane	1076:1114	Because the MWCNTs played a role of free channels for water molecules, the composite membrane water flux reached to threefold flux of the pristine membrane.					
31995891	5	20	theme	@	934:934	arg1	surface					951:957	the PES-Ni@MWCNTs membrane surface	924:957	the PES-Ni@MWCNTs membrane surface	924:957	The morphology measurements confirmed that the Ni@MWCNTs headed out of the PES-Ni@MWCNTs membrane surface.					
31995891	2	21	theme	YE	500:501	arg1	solutions					504:512	yeast (YE) solutions	493:512	yeast (YE) solutions	493:512	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	2	22	theme	yeast	493:497	arg1	solutions					504:512	yeast (YE) solutions	493:512	yeast (YE) solutions	493:512	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	1	23	theme	wall	127:130	arg1	MWCNTs					150:155	MWCNTs	150:155	MWCNTs	150:155	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	1	23	theme	wall	127:130	arg1	nanotubes					139:147	Multiple wall carbon nanotubes	118:147	Multiple wall carbon nanotubes (MWCNTs)	118:156	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	4	24	theme	membrane	770:777	arg1	surface					779:785	the membrane surface	766:785	the membrane surface	766:785	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	6	25	theme	water	1054:1058	arg1	flux					1060:1063	the composite membrane water flux	1031:1063	the composite membrane water flux	1031:1063	Because the MWCNTs played a role of free channels for water molecules, the composite membrane water flux reached to threefold flux of the pristine membrane.					
31995891	4	26	theme	inversion	834:842	arg1	process					844:850	the phase inversion process	824:850	the phase inversion process	824:850	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	6	27	theme	composite	1035:1043	arg1	flux					1060:1063	the composite membrane water flux	1031:1063	the composite membrane water flux	1031:1063	Because the MWCNTs played a role of free channels for water molecules, the composite membrane water flux reached to threefold flux of the pristine membrane.					
31995891	9	28	theme	interaction	1749:1759	arg1	energies					1761:1768	advantageous interaction energies	1736:1768	advantageous interaction energies	1736:1768	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	0	29	theme	membrane	53:60	arg1	permeability					76:87	membrane with enhanced permeability	53:87	membrane with enhanced permeability	53:87	Magnetic field assisted preparation of PES-Ni@MWCNTs membrane with enhanced permeability and antifouling performance.					
31995891	8	30	theme	pristine	1513:1520	arg1	membrane					1522:1529	the pristine membrane	1509:1529	the pristine membrane	1509:1529	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	3	31	from	Ag+	659:661	arg1	MWCNTs					666:671	MWCNTs	666:671	MWCNTs	666:671	In this study, the Ni@MWCNTs was generated by immersing MWCNTs into Ni2+ solution where in-situ reduction reaction was launched by the adsorbed Ag+ on MWCNTs.					
31995891	0	32	theme	enhanced	67:74	arg1	permeability					76:87	membrane with enhanced permeability	53:87	membrane with enhanced permeability	53:87	Magnetic field assisted preparation of PES-Ni@MWCNTs membrane with enhanced permeability and antifouling performance.					
31995891	8	33	theme	previous	1595:1602	arg1	studies					1604:1610	the previous studies	1591:1610	the previous studies	1591:1610	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	2	34	theme	serum	469:473	arg1	BSA					484:486	BSA	484:486	BSA	484:486	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	2	34	theme	serum	469:473	arg1	albumin					475:481	bovine serum albumin	462:481	bovine serum albumin (BSA)	462:487	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	8	35	theme	optimal	1319:1325	arg1	membrane					1341:1348	the optimal PES-Ni@MWCNTs membrane	1315:1348	the optimal PES-Ni@MWCNTs membrane	1315:1348	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	5	36	theme	membrane	942:949	arg1	surface					951:957	the PES-Ni@MWCNTs membrane surface	924:957	the PES-Ni@MWCNTs membrane surface	924:957	The morphology measurements confirmed that the Ni@MWCNTs headed out of the PES-Ni@MWCNTs membrane surface.					
31995891	8	37	theme	@	1333:1333	arg1	membrane					1341:1348	the optimal PES-Ni@MWCNTs membrane	1315:1348	the optimal PES-Ni@MWCNTs membrane	1315:1348	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	9	38	theme	modified	1708:1715	arg1	membrane					1717:1724	the modified membrane	1704:1724	the modified membrane	1704:1724	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	8	39	theme	significant	1551:1561	arg1	improvements					1563:1574	significant improvements	1551:1574	significant improvements comparing with the previous studies	1551:1610	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	2	40	theme	PES	367:369	arg1	membrane					372:379	polyether sulfone (PES) membrane	348:379	polyether sulfone (PES) membrane	348:379	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	0	41	theme	Magnetic	0:7	arg1	preparation					24:34	Magnetic field assisted preparation	0:34	Magnetic field assisted preparation of PES-Ni@	0:45	Magnetic field assisted preparation of PES-Ni@MWCNTs membrane with enhanced permeability and antifouling performance.					
31995891	1	42	theme	membranes	274:282	arg1	preparation					234:244	preparation	234:244	preparation of polymer-MWCNTs composite membranes	234:282	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	7	43	theme	enhanced	1179:1186	arg1	ability					1200:1206	the obviously enhanced antifouling ability	1165:1206	the obviously enhanced antifouling ability	1165:1206	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	2	44	theme	antifouling	396:406	arg1	performance					408:418	its antifouling performance	392:418	its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions	392:512	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	0	45	theme	assisted	15:22	arg1	preparation					24:34	Magnetic field assisted preparation	0:34	Magnetic field assisted preparation of PES-Ni@	0:45	Magnetic field assisted preparation of PES-Ni@MWCNTs membrane with enhanced permeability and antifouling performance.					
31995891	7	46	theme	MWCNTs	1138:1143	arg1	membranes					1145:1153	the PES-Ni@MWCNTs membranes	1127:1153	the PES-Ni@MWCNTs membranes	1127:1153	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	8	47	theme	recovery	1370:1377	arg1	higher					1489:1494	higher	1489:1494	higher	1489:1494	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	47	theme	recovery	1370:1377	arg1	FRR					1385:1387	FRR	1385:1387	FRR	1385:1387	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	47	theme	recovery	1370:1377	arg1	rate					1379:1382	a flux recovery rate	1363:1382	a flux recovery rate (FRR)	1363:1388	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	7	48	theme	PES-Ni	1131:1136	arg1	membranes					1145:1153	the PES-Ni@MWCNTs membranes	1127:1153	the PES-Ni@MWCNTs membranes	1127:1153	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	3	49	theme	@	536:536	arg1	MWCNTs					537:542	the Ni@MWCNTs	530:542	the Ni@MWCNTs	530:542	In this study, the Ni@MWCNTs was generated by immersing MWCNTs into Ni2+ solution where in-situ reduction reaction was launched by the adsorbed Ag+ on MWCNTs.					
31995891	2	50	theme	@	335:335	arg1	MWCNTs					336:341	the magnetic Ni@MWCNTs	320:341	the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane	320:379	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	0	51	theme	@	45:45	arg1	preparation					24:34	Magnetic field assisted preparation	0:34	Magnetic field assisted preparation of PES-Ni@	0:45	Magnetic field assisted preparation of PES-Ni@MWCNTs membrane with enhanced permeability and antifouling performance.					
31995891	2	52	theme	magnetic	324:331	arg1	MWCNTs					336:341	the magnetic Ni@MWCNTs	320:341	the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane	320:379	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	5	53	theme	morphology	857:866	arg1	measurements					868:879	The morphology measurements	853:879	The morphology measurements	853:879	The morphology measurements confirmed that the Ni@MWCNTs headed out of the PES-Ni@MWCNTs membrane surface.					
31995891	4	54	theme	Ni	691:692	arg1	magnetism					702:710	the loaded Ni endowed magnetism	680:710	the loaded Ni endowed magnetism to MWCNTs	680:720	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	2	55	theme	sulfone	358:364	arg1	membrane					372:379	polyether sulfone (PES) membrane	348:379	polyether sulfone (PES) membrane	348:379	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	7	56	theme	solutions	1291:1299	arg1	cycles					1252:1257	all the three alternative filtration cycles	1215:1257	all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions	1215:1299	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	8	57	theme	%	1406:1406	arg1	higher					1489:1494	higher	1489:1494	higher	1489:1494	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	57	theme	%	1406:1406	arg1	FRR					1385:1387	FRR	1385:1387	FRR	1385:1387	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	57	theme	%	1406:1406	arg1	rate					1379:1382	a flux recovery rate	1363:1382	a flux recovery rate (FRR)	1363:1388	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	3	58	theme	Ni2+	583:586	arg1	solution					588:595	Ni2+ solution	583:595	Ni2+ solution where in-situ reduction reaction was launched by the adsorbed Ag+ on MWCNTs	583:671	In this study, the Ni@MWCNTs was generated by immersing MWCNTs into Ni2+ solution where in-situ reduction reaction was launched by the adsorbed Ag+ on MWCNTs.					
31995891	1	59	theme	polymer-MWCNTs	249:262	arg1	membranes					274:282	polymer-MWCNTs composite membranes	249:282	polymer-MWCNTs composite membranes	249:282	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	9	60	theme	extended	1632:1639	arg1	theory					1682:1687	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	1632:1687	extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory	1632:1687	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	6	61	theme	pristine	1098:1105	arg1	membrane					1107:1114	the pristine membrane	1094:1114	the pristine membrane	1094:1114	Because the MWCNTs played a role of free channels for water molecules, the composite membrane water flux reached to threefold flux of the pristine membrane.					
31995891	7	62	theme	BSA	1272:1274	arg1	cycles					1252:1257	all the three alternative filtration cycles	1215:1257	all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions	1215:1299	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	3	63	theme	reduction	611:619	arg1	reaction					621:628	in-situ reduction reaction	603:628	in-situ reduction reaction	603:628	In this study, the Ni@MWCNTs was generated by immersing MWCNTs into Ni2+ solution where in-situ reduction reaction was launched by the adsorbed Ag+ on MWCNTs.					
31995891	1	64	theme	excellent	165:173	arg1	material					175:182	an excellent material	162:182	an excellent material	162:182	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	4	65	theme	Ni	727:728	arg1	MWCNTs					730:735	the Ni@MWCNTs	723:735	the Ni@MWCNTs	723:735	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	7	66	theme	water	1262:1266	arg1	cycles					1252:1257	all the three alternative filtration cycles	1215:1257	all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions	1215:1299	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	3	67	theme	adsorbed	650:657	arg1	Ag+					659:661	the adsorbed Ag+	646:661	the adsorbed Ag+ on MWCNTs	646:671	In this study, the Ni@MWCNTs was generated by immersing MWCNTs into Ni2+ solution where in-situ reduction reaction was launched by the adsorbed Ag+ on MWCNTs.					
31995891	6	68	theme	free	996:999	arg1	channels					1001:1008	free channels	996:1008	free channels	996:1008	Because the MWCNTs played a role of free channels for water molecules, the composite membrane water flux reached to threefold flux of the pristine membrane.					
31995891	5	69	theme	PES-Ni	928:933	arg1	surface					951:957	the PES-Ni@MWCNTs membrane surface	924:957	the PES-Ni@MWCNTs membrane surface	924:957	The morphology measurements confirmed that the Ni@MWCNTs headed out of the PES-Ni@MWCNTs membrane surface.					
31995891	7	70	theme	alternative	1229:1239	arg1	cycles					1252:1257	all the three alternative filtration cycles	1215:1257	all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions	1215:1299	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	5	71	theme	MWCNTs	935:940	arg1	surface					951:957	the PES-Ni@MWCNTs membrane surface	924:957	the PES-Ni@MWCNTs membrane surface	924:957	The morphology measurements confirmed that the Ni@MWCNTs headed out of the PES-Ni@MWCNTs membrane surface.					
31995891	1	72	theme	Multiple	118:125	arg1	MWCNTs					150:155	MWCNTs	150:155	MWCNTs	150:155	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	1	72	theme	Multiple	118:125	arg1	nanotubes					139:147	Multiple wall carbon nanotubes	118:147	Multiple wall carbon nanotubes (MWCNTs)	118:156	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	1	73	theme	carbon	132:137	arg1	MWCNTs					150:155	MWCNTs	150:155	MWCNTs	150:155	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	1	73	theme	carbon	132:137	arg1	nanotubes					139:147	Multiple wall carbon nanotubes	118:147	Multiple wall carbon nanotubes (MWCNTs)	118:156	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	6	74	theme	membrane	1045:1052	arg1	flux					1060:1063	the composite membrane water flux	1031:1063	the composite membrane water flux	1031:1063	Because the MWCNTs played a role of free channels for water molecules, the composite membrane water flux reached to threefold flux of the pristine membrane.					
31995891	9	75	theme	advantageous	1736:1747	arg1	energies					1761:1768	advantageous interaction energies	1736:1768	advantageous interaction energies	1736:1768	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	0	76	theme	with	62:65	arg1	permeability					76:87	membrane with enhanced permeability	53:87	membrane with enhanced permeability	53:87	Magnetic field assisted preparation of PES-Ni@MWCNTs membrane with enhanced permeability and antifouling performance.					
31995891	4	77	theme	phase	828:832	arg1	process					844:850	the phase inversion process	824:850	the phase inversion process	824:850	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	7	78	theme	antifouling	1188:1198	arg1	ability					1200:1206	the obviously enhanced antifouling ability	1165:1206	the obviously enhanced antifouling ability	1165:1206	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	4	79	theme	magnetic	802:809	arg1	field					811:815	an external magnetic field	790:815	an external magnetic field during the phase inversion process	790:850	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	2	80	theme	sodium	440:445	arg1	SA					457:458	SA	457:458	SA	457:458	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	2	80	theme	sodium	440:445	arg1	alginate					447:454	sodium alginate	440:454	sodium alginate (SA)	440:459	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	8	81	theme	PES-Ni	1327:1332	arg1	membrane					1341:1348	the optimal PES-Ni@MWCNTs membrane	1315:1348	the optimal PES-Ni@MWCNTs membrane	1315:1348	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	9	82	theme	contaminant	1775:1785	arg1	molecules					1787:1795	contaminant molecules	1775:1795	contaminant molecules over the pristine membrane	1775:1822	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	0	83	theme	antifouling	93:103	arg1	performance					105:115	antifouling performance	93:115	antifouling performance	93:115	Magnetic field assisted preparation of PES-Ni@MWCNTs membrane with enhanced permeability and antifouling performance.					
31995891	2	84	theme	bovine	462:467	arg1	BSA					484:486	BSA	484:486	BSA	484:486	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	2	84	theme	bovine	462:467	arg1	albumin					475:481	bovine serum albumin	462:481	bovine serum albumin (BSA)	462:487	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	8	85	theme	MWCNTs	1334:1339	arg1	membrane					1341:1348	the optimal PES-Ni@MWCNTs membrane	1315:1348	the optimal PES-Ni@MWCNTs membrane	1315:1348	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	9	86	theme	pristine	1806:1813	arg1	membrane					1815:1822	the pristine membrane	1802:1822	the pristine membrane	1802:1822	Further results of extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory indicated that the modified membrane possessed advantageous interaction energies with contaminant molecules over the pristine membrane.					
31995891	0	87	theme	field	9:13	arg1	preparation					24:34	Magnetic field assisted preparation	0:34	Magnetic field assisted preparation of PES-Ni@	0:45	Magnetic field assisted preparation of PES-Ni@MWCNTs membrane with enhanced permeability and antifouling performance.					
31995891	2	88	theme	humic	423:427	arg1	HA					435:436	HA	435:436	HA	435:436	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	2	88	theme	humic	423:427	arg1	acid					429:432	humic acid	423:432	humic acid (HA)	423:437	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	8	89	theme	flux	1365:1368	arg1	higher					1489:1494	higher	1489:1494	higher	1489:1494	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	89	theme	flux	1365:1368	arg1	FRR					1385:1387	FRR	1385:1387	FRR	1385:1387	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	89	theme	flux	1365:1368	arg1	rate					1379:1382	a flux recovery rate	1363:1382	a flux recovery rate (FRR)	1363:1388	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	1	90	used	used	195:198	arg2	MWCNTs					150:155	MWCNTs	150:155	MWCNTs	150:155	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	1	90	used	used	195:198	arg2	nanotubes					139:147	Multiple wall carbon nanotubes	118:147	Multiple wall carbon nanotubes (MWCNTs)	118:156	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	7	91	theme	@	1137:1137	arg1	membranes					1145:1153	the PES-Ni@MWCNTs membranes	1127:1153	the PES-Ni@MWCNTs membranes	1127:1153	Moreover, the PES-Ni@MWCNTs membranes displayed the obviously enhanced antifouling ability during all the three alternative filtration cycles of water and BSA, SA, YE and HA solutions.					
31995891	0	92	theme	PES-Ni	39:44	arg1	@					45:45	PES-Ni@	39:45	PES-Ni@	39:45	Magnetic field assisted preparation of PES-Ni@MWCNTs membrane with enhanced permeability and antifouling performance.					
31995891	3	93	theme	Ni	534:535	arg1	MWCNTs					537:542	the Ni@MWCNTs	530:542	the Ni@MWCNTs	530:542	In this study, the Ni@MWCNTs was generated by immersing MWCNTs into Ni2+ solution where in-situ reduction reaction was launched by the adsorbed Ag+ on MWCNTs.					
31995891	4	94	theme	endowed	694:700	arg1	magnetism					702:710	the loaded Ni endowed magnetism	680:710	the loaded Ni endowed magnetism to MWCNTs	680:720	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	5	95	theme	Ni	900:901	arg1	@					902:902	the Ni@	896:902	the Ni@	896:902	The morphology measurements confirmed that the Ni@MWCNTs headed out of the PES-Ni@MWCNTs membrane surface.					
31995891	2	96	theme	Ni	333:334	arg1	MWCNTs					336:341	the magnetic Ni@MWCNTs	320:341	the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane	320:379	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	8	97	theme	%	1424:1424	arg1	higher					1489:1494	higher	1489:1494	higher	1489:1494	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	97	theme	%	1424:1424	arg1	FRR					1385:1387	FRR	1385:1387	FRR	1385:1387	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	97	theme	%	1424:1424	arg1	rate					1379:1382	a flux recovery rate	1363:1382	a flux recovery rate (FRR)	1363:1388	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	2	98	with	MWCNTs	336:341	arg1	membrane					372:379	polyether sulfone (PES) membrane	348:379	polyether sulfone (PES) membrane	348:379	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	8	99	theme	%	1398:1398	arg1	higher					1489:1494	higher	1489:1494	higher	1489:1494	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	99	theme	%	1398:1398	arg1	FRR					1385:1387	FRR	1385:1387	FRR	1385:1387	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	99	theme	%	1398:1398	arg1	rate					1379:1382	a flux recovery rate	1363:1382	a flux recovery rate (FRR)	1363:1388	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	1	100	theme	various	203:209	arg1	applications					211:222	various applications	203:222	various applications including preparation of polymer-MWCNTs composite membranes	203:282	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	1	100	theme	various	203:209	arg1	preparation					234:244	preparation	234:244	preparation of polymer-MWCNTs composite membranes	234:282	Multiple wall carbon nanotubes (MWCNTs), as an excellent material, have been used in various applications including preparation of polymer-MWCNTs composite membranes.					
31995891	4	101	theme	loaded	684:689	arg1	magnetism					702:710	the loaded Ni endowed magnetism	680:710	the loaded Ni endowed magnetism to MWCNTs	680:720	Since the loaded Ni endowed magnetism to MWCNTs, the Ni@MWCNTs can be easily attracted onto the membrane surface by an external magnetic field during the phase inversion process.					
31995891	2	102	theme	polyether	348:356	arg1	membrane					372:379	polyether sulfone (PES) membrane	348:379	polyether sulfone (PES) membrane	348:379	However, few reports have combined the magnetic Ni@MWCNTs with polyether sulfone (PES) membrane to improve its antifouling performance to humic acid (HA), sodium alginate (SA), bovine serum albumin (BSA) and yeast (YE) solutions.					
31995891	8	103	theme	SA	1435:1436	arg1	higher					1489:1494	higher	1489:1494	higher	1489:1494	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	103	theme	SA	1435:1436	arg1	FRR					1385:1387	FRR	1385:1387	FRR	1385:1387	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31995891	8	103	theme	SA	1435:1436	arg1	rate					1379:1382	a flux recovery rate	1363:1382	a flux recovery rate (FRR)	1363:1388	In addition, the optimal PES-Ni@MWCNTs membrane demonstrated a flux recovery rate (FRR) of 67.89%, 85.53%, 60.28 and 90.12% for BSA, SA, YE and HA, respectively, which were not only much higher than that of the pristine membrane, but also exhibited significant improvements comparing with the previous studies.					
31712156	8	0	contain	containing	1246:1255	arg2	HEC					1257:1259	HEC	1257:1259	HEC	1257:1259	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	8	0	contain	containing	1246:1255	arg1	formulations					1233:1244	two selected formulations	1220:1244	two selected formulations containing HEC (F7)	1220:1264	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	8	0	contain	containing	1246:1255	arg2	F7					1262:1263	F7	1262:1263	F7	1262:1263	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	7	1	theme	mechanical	1103:1112	arg1	characters					1114:1123	bioadhesive and mechanical characters	1087:1123	bioadhesive and mechanical characters	1087:1123	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	12	2	theme	healing	1891:1897	arg1	dressing					1899:1906	an efficient wound healing dressing	1872:1906	an efficient wound healing dressing with antimicrobial and anti-inflammatory activities	1872:1958	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	6	3	theme	swelling	892:899	arg1	fraction					882:889	gel fraction	878:889	gel fraction	878:889	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	6	3	theme	swelling	892:899	arg1	ratio					901:905	swelling ratio	892:905	swelling ratio	892:905	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	4	4	theme	freezing-thawing	609:624	arg1	method					626:631	the freezing-thawing method	605:631	the freezing-thawing method	605:631	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc were developed using the freezing-thawing method.					
31712156	7	5	theme	bioadhesive	1087:1097	arg1	characters					1114:1123	bioadhesive and mechanical characters	1087:1123	bioadhesive and mechanical characters	1087:1123	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	6	6	theme	fraction	882:889	arg1	determination					861:873	determination	861:873	determination of gel fraction, swelling ratio, mechanical and bioadhesive properties	861:944	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	2	7	theme	prepared	279:286	arg1	polysaccharide					288:301	The prepared polysaccharide	275:301	The prepared polysaccharide	275:301	The prepared polysaccharide showed high β-glucan content which accelerates wound healing.					
31712156	1	8	theme	healing	170:176	arg1	hydrogel					203:210	an efficient wound healing PVA-biopolymer composite hydrogel	151:210	an efficient wound healing PVA-biopolymer composite hydrogel	151:210	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	7	9	theme	better	1064:1069	arg1	ratio					1080:1084	better swelling ratio	1064:1084	better swelling ratio	1064:1084	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	1	10	theme	composite	193:201	arg1	hydrogel					203:210	an efficient wound healing PVA-biopolymer composite hydrogel	151:210	an efficient wound healing PVA-biopolymer composite hydrogel	151:210	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	12	11	theme	efficient	1875:1883	arg1	healing					1891:1897	an efficient wound healing	1872:1897	an efficient wound healing dressing with antimicrobial and anti-inflammatory activities	1872:1958	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	10	12	theme	higher	1549:1554	arg1	activity					1574:1581	higher anti-inflammatory activity	1549:1581	higher anti-inflammatory activity of F7 (21.4% edema reduction)	1549:1611	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	11	13	theme	size	1711:1714	arg1	healing					1683:1689	better healing	1676:1689	better healing (99% relative wound size reduction)	1676:1725	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	11	13	theme	size	1711:1714	arg1	reduction					1716:1724	99% relative wound size reduction	1692:1724	99% relative wound size reduction	1692:1724	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	2	14	theme	β-glucan	315:322	arg1	content					324:330	high β-glucan content	310:330	high β-glucan content which accelerates wound healing	310:362	The prepared polysaccharide showed high β-glucan content which accelerates wound healing.					
31712156	1	15	dep	Avena	259:263	arg1	L					272:272	L	272:272	Egyptian Avena sativa L	250:272	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	1	15	dep	Avena	259:263	arg1	sativa					265:270	Avena sativa L	259:272	Egyptian Avena sativa L	250:272	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	10	16	theme	edema	1596:1600	arg1	reduction					1602:1610	21.4% edema reduction	1590:1610	21.4% edema reduction	1590:1610	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	10	16	theme	edema	1596:1600	arg1	F7					1586:1587	F7	1586:1587	F7 (21.4% edema reduction)	1586:1611	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	1	17	attach	derived	237:243	arg2	polysaccharide					222:235	the polysaccharide	218:235	the polysaccharide derived from Egyptian Avena sativa L	218:272	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	1	17	attach	derived	237:243	arg1	Avena					259:263	Avena	259:263	Avena	259:263	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	9	18	theme	anti-inflammatory	1425:1441	arg1	evaluation					1407:1416	In vivo evaluation	1399:1416	In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products	1399:1504	In addition, In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products were performed on rats.					
31712156	10	19	theme	F7	1586:1587	arg1	activity					1574:1581	higher anti-inflammatory activity	1549:1581	higher anti-inflammatory activity of F7 (21.4% edema reduction)	1549:1611	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	7	20	theme	hydrophilic	1030:1040	arg1	polymers					1048:1055	anionic (NaCMC and CP) and more hydrophilic (HEC) polymers	998:1055	anionic (NaCMC and CP) and more hydrophilic (HEC) polymers	998:1055	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	9	21	theme	wound	1447:1451	arg1	healing					1453:1459	wound healing	1447:1459	wound healing	1447:1459	In addition, In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products were performed on rats.					
31712156	9	22	theme	healing	1453:1459	arg1	evaluation					1407:1416	In vivo evaluation	1399:1416	In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products	1399:1504	In addition, In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products were performed on rats.					
31712156	11	23	theme	relative	1696:1703	arg1	healing					1683:1689	better healing	1676:1689	better healing (99% relative wound size reduction)	1676:1725	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	11	23	theme	relative	1696:1703	arg1	reduction					1716:1724	99% relative wound size reduction	1692:1724	99% relative wound size reduction	1692:1724	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	12	24	theme	Egyptian	1805:1812	arg1	polysaccharide					1830:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	1	25	theme	efficient	154:162	arg1	healing					170:176	an efficient wound healing	151:176	an efficient wound healing PVA-biopolymer composite hydrogel	151:210	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	4	26	theme	different	528:536	arg1	polymers					538:545	different polymers	528:545	different polymers	528:545	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc were developed using the freezing-thawing method.					
31712156	7	27	dep	cationic	1164:1171	arg1	PVP					1174:1176	PVP	1174:1176	PVP	1174:1176	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	7	27	dep	cationic	1164:1171	arg1	HPMC					1200:1203	HPMC	1200:1203	HPMC	1200:1203	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	8	28	theme	disk	1282:1285	arg1	method					1297:1302	disk diffusion method	1282:1302	disk diffusion method	1282:1302	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	12	29	theme	sativa	1820:1825	arg1	polysaccharide					1830:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	3	30	theme	β-glucan	369:376	arg1	content					378:384	The β-glucan content	365:384	The β-glucan content	365:384	The β-glucan content was 13.28% and GC analysis revealed that glucose was the major sugar component (71.19%).					
31712156	3	30	theme	β-glucan	369:376	arg1	%					395:395	13.28%	390:395	13.28%	390:395	The β-glucan content was 13.28% and GC analysis revealed that glucose was the major sugar component (71.19%).					
31712156	3	31	theme	sugar	449:453	arg1	glucose					427:433	glucose	427:433	glucose	427:433	The β-glucan content was 13.28% and GC analysis revealed that glucose was the major sugar component (71.19%).					
31712156	3	31	theme	sugar	449:453	arg1	component					455:463	the major sugar component	439:463	the major sugar component (71.19%)	439:472	The β-glucan content was 13.28% and GC analysis revealed that glucose was the major sugar component (71.19%).					
31712156	3	31	theme	sugar	449:453	arg1	%					471:471	71.19%	466:471	71.19%	466:471	The β-glucan content was 13.28% and GC analysis revealed that glucose was the major sugar component (71.19%).					
31712156	5	32	theme	hydroxypropyl	734:746	arg1	HPMC					765:768	HPMC	765:768	HPMC	765:768	The used polymers were; polyvinylpyrrolidone (PVP), Carbopol 940 (CP), hydroxyethylcellulose (HEC), hydroxypropyl methylcellulose (HPMC), and sodium carboxymethylcellulose (Na CMC).					
31712156	5	32	theme	hydroxypropyl	734:746	arg1	methylcellulose					748:762	hydroxypropyl methylcellulose	734:762	hydroxypropyl methylcellulose (HPMC)	734:769	The used polymers were; polyvinylpyrrolidone (PVP), Carbopol 940 (CP), hydroxyethylcellulose (HEC), hydroxypropyl methylcellulose (HPMC), and sodium carboxymethylcellulose (Na CMC).					
31712156	2	33	theme	wound	350:354	arg1	healing					356:362	wound healing	350:362	wound healing	350:362	The prepared polysaccharide showed high β-glucan content which accelerates wound healing.					
31712156	3	34	theme	GC	401:402	arg1	analysis					404:411	GC analysis	401:411	GC analysis	401:411	The β-glucan content was 13.28% and GC analysis revealed that glucose was the major sugar component (71.19%).					
31712156	4	35	theme	PVA-polysaccharide	485:502	arg1	hydrogels					504:512	Different PVA-polysaccharide hydrogels	475:512	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc	475:582	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc were developed using the freezing-thawing method.					
31712156	8	36	theme	In	1308:1309	arg1	penetration					1327:1337	In vitro microbial penetration	1308:1337	In vitro microbial penetration	1308:1337	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	0	37	theme	Efficient	0:8	arg1	healing					16:22	Efficient wound healing	0:22	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide	0:87	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide containing β-glucan.					
31712156	4	38	dep	hydrogels	504:512	arg1	loaded					551:556	loaded	551:556	loaded with 0.3% bacitracin zinc	551:582	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc were developed using the freezing-thawing method.					
31712156	4	38	dep	hydrogels	504:512	arg1	combined					514:521	combined	514:521	combined with different polymers	514:545	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc were developed using the freezing-thawing method.					
31712156	4	39	theme	%	566:566	arg1	zinc					579:582	0.3% bacitracin zinc	563:582	0.3% bacitracin zinc	563:582	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc were developed using the freezing-thawing method.					
31712156	8	40	theme	microbial	1317:1325	arg1	penetration					1327:1337	In vitro microbial penetration	1308:1337	In vitro microbial penetration	1308:1337	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	0	41	theme	healing	16:22	arg1	hydrogel					34:41	Efficient wound healing composite hydrogel	0:41	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide	0:87	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide containing β-glucan.					
31712156	9	42	dep	In	1399:1400	arg1	vivo					1402:1405	vivo	1402:1405	vivo	1402:1405	In addition, In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products were performed on rats.					
31712156	7	43	dep	anionic	998:1004	arg1	HEC					1043:1045	HEC	1043:1045	HEC	1043:1045	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	7	43	dep	anionic	998:1004	arg1	CP					1017:1018	CP	1017:1018	CP	1017:1018	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	7	43	dep	anionic	998:1004	arg1	NaCMC					1007:1011	NaCMC	1007:1011	NaCMC	1007:1011	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	0	44	theme	Egyptian	49:56	arg1	polysaccharide					74:87	Egyptian Avena sativa L. polysaccharide	49:87	Egyptian Avena sativa L. polysaccharide	49:87	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide containing β-glucan.					
31712156	10	45	theme	edema	1637:1641	arg1	reduction					1643:1651	19.8% edema reduction	1631:1651	19.8% edema reduction	1631:1651	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	10	45	theme	edema	1637:1641	arg1	F9					1627:1628	F9	1627:1628	F9 (19.8% edema reduction)	1627:1652	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	5	46	theme	sodium	776:781	arg1	carboxymethylcellulose					783:804	sodium carboxymethylcellulose	776:804	sodium carboxymethylcellulose (Na CMC)	776:813	The used polymers were; polyvinylpyrrolidone (PVP), Carbopol 940 (CP), hydroxyethylcellulose (HEC), hydroxypropyl methylcellulose (HPMC), and sodium carboxymethylcellulose (Na CMC).					
31712156	5	46	theme	sodium	776:781	arg1	CMC					810:812	Na CMC	807:812	Na CMC	807:812	The used polymers were; polyvinylpyrrolidone (PVP), Carbopol 940 (CP), hydroxyethylcellulose (HEC), hydroxypropyl methylcellulose (HPMC), and sodium carboxymethylcellulose (Na CMC).					
31712156	6	47	theme	mechanical	908:917	arg1	properties					935:944	mechanical and bioadhesive properties	908:944	mechanical and bioadhesive properties	908:944	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	6	47	theme	mechanical	908:917	arg1	fraction					882:889	gel fraction	878:889	gel fraction	878:889	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	12	48	theme	anti-inflammatory	1931:1947	arg1	activities					1949:1958	anti-inflammatory activities	1931:1958	anti-inflammatory activities	1931:1958	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	2	49	theme	high	310:313	arg1	content					324:330	high β-glucan content	310:330	high β-glucan content which accelerates wound healing	310:362	The prepared polysaccharide showed high β-glucan content which accelerates wound healing.					
31712156	12	50	theme	wound	1885:1889	arg1	healing					1891:1897	an efficient wound healing	1872:1897	an efficient wound healing dressing with antimicrobial and anti-inflammatory activities	1872:1958	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	7	51	theme	swelling	1071:1078	arg1	ratio					1080:1084	better swelling ratio	1064:1084	better swelling ratio	1064:1084	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	1	52	theme	PVA-biopolymer	178:191	arg1	hydrogel					203:210	an efficient wound healing PVA-biopolymer composite hydrogel	151:210	an efficient wound healing PVA-biopolymer composite hydrogel	151:210	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	1	53	theme	wound	164:168	arg1	healing					170:176	an efficient wound healing	151:176	an efficient wound healing PVA-biopolymer composite hydrogel	151:210	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	12	54	theme	dressing	1899:1906	arg1	development					1857:1867	development	1857:1867	development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities	1857:1958	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	6	55	theme	gel	878:880	arg1	fraction					882:889	gel fraction	878:889	gel fraction	878:889	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	6	55	theme	gel	878:880	arg1	properties					935:944	mechanical and bioadhesive properties	908:944	mechanical and bioadhesive properties	908:944	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	6	55	theme	gel	878:880	arg1	ratio					901:905	swelling ratio	892:905	swelling ratio	892:905	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	8	56	dep	In	1308:1309	arg1	vitro					1311:1315	vitro	1311:1315	vitro	1311:1315	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	10	57	theme	anti-inflammatory	1556:1572	arg1	activity					1574:1581	higher anti-inflammatory activity	1549:1581	higher anti-inflammatory activity of F7 (21.4% edema reduction)	1549:1611	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	8	58	theme	selected	1224:1231	arg1	formulations					1233:1244	two selected formulations	1220:1244	two selected formulations containing HEC (F7)	1220:1264	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	10	59	theme	%	1594:1594	arg1	reduction					1602:1610	21.4% edema reduction	1590:1610	21.4% edema reduction	1590:1610	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	10	59	theme	%	1594:1594	arg1	F7					1586:1587	F7	1586:1587	F7 (21.4% edema reduction)	1586:1611	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	6	60	theme	bioadhesive	923:933	arg1	properties					935:944	mechanical and bioadhesive properties	908:944	mechanical and bioadhesive properties	908:944	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	6	60	theme	bioadhesive	923:933	arg1	fraction					882:889	gel fraction	878:889	gel fraction	878:889	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	12	61	with	dressing	1899:1906	arg1	antimicrobial					1913:1925	antimicrobial	1913:1925	antimicrobial	1913:1925	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	12	61	with	dressing	1899:1906	arg1	activities					1949:1958	anti-inflammatory activities	1931:1958	anti-inflammatory activities	1931:1958	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	1	62	theme	study	130:134	arg1	purpose					114:120	The purpose	110:120	The purpose of this study	110:134	The purpose of this study was to develop an efficient wound healing PVA-biopolymer composite hydrogel using the polysaccharide derived from Egyptian Avena sativa L.					
31712156	9	63	theme	In	1399:1400	arg1	evaluation					1407:1416	In vivo evaluation	1399:1416	In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products	1399:1504	In addition, In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products were performed on rats.					
31712156	7	64	theme	anionic	998:1004	arg1	polymers					1048:1055	anionic (NaCMC and CP) and more hydrophilic (HEC) polymers	998:1055	anionic (NaCMC and CP) and more hydrophilic (HEC) polymers	998:1055	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	11	65	theme	better	1676:1681	arg1	healing					1683:1689	better healing	1676:1689	better healing (99% relative wound size reduction)	1676:1725	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	11	65	theme	better	1676:1681	arg1	reduction					1716:1724	99% relative wound size reduction	1692:1724	99% relative wound size reduction	1692:1724	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	7	66	theme	hydrophilic	1187:1197	arg1	polymers					1206:1213	cationic (PVP) or less hydrophilic (HPMC) polymers	1164:1213	cationic (PVP) or less hydrophilic (HPMC) polymers	1164:1213	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	12	67	theme	Avena	1814:1818	arg1	polysaccharide					1830:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	9	68	theme	conventional	1484:1495	arg1	products					1497:1504	conventional products	1484:1504	conventional products	1484:1504	In addition, In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products were performed on rats.					
31712156	11	69	theme	wound	1705:1709	arg1	healing					1683:1689	better healing	1676:1689	better healing (99% relative wound size reduction)	1676:1725	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	11	69	theme	wound	1705:1709	arg1	reduction					1716:1724	99% relative wound size reduction	1692:1724	99% relative wound size reduction	1692:1724	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	8	70	theme	microbiological	1358:1372	arg1	assessment					1374:1383	microbiological assessment	1358:1383	microbiological assessment	1358:1383	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	12	71	theme	L.	1827:1828	arg1	polysaccharide					1830:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	4	72	theme	Different	475:483	arg1	hydrogels					504:512	Different PVA-polysaccharide hydrogels	475:512	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc	475:582	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc were developed using the freezing-thawing method.					
31712156	11	73	theme	%	1694:1694	arg1	healing					1683:1689	better healing	1676:1689	better healing (99% relative wound size reduction)	1676:1725	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	11	73	theme	%	1694:1694	arg1	reduction					1716:1724	99% relative wound size reduction	1692:1724	99% relative wound size reduction	1692:1724	Similarly, F7 showed better healing (99% relative wound size reduction) than F9 (75%).					
31712156	5	74	theme	used	638:641	arg1	polymers					643:650	The used polymers	634:650	The used polymers	634:650	The used polymers were; polyvinylpyrrolidone (PVP), Carbopol 940 (CP), hydroxyethylcellulose (HEC), hydroxypropyl methylcellulose (HPMC), and sodium carboxymethylcellulose (Na CMC).					
31712156	5	74	theme	used	638:641	arg1	polyvinylpyrrolidone					658:677	polyvinylpyrrolidone	658:677	polyvinylpyrrolidone (PVP)	658:683	The used polymers were; polyvinylpyrrolidone (PVP), Carbopol 940 (CP), hydroxyethylcellulose (HEC), hydroxypropyl methylcellulose (HPMC), and sodium carboxymethylcellulose (Na CMC).					
31712156	8	75	theme	diffusion	1287:1295	arg1	method					1297:1302	disk diffusion method	1282:1302	disk diffusion method	1282:1302	For two selected formulations containing HEC (F7) and NaCMC (F9), disk diffusion method and In vitro microbial penetration were performed for microbiological assessment.					
31712156	7	76	theme	cationic	1164:1171	arg1	polymers					1206:1213	cationic (PVP) or less hydrophilic (HPMC) polymers	1164:1213	cationic (PVP) or less hydrophilic (HPMC) polymers	1164:1213	The results revealed that hydrogels prepared using anionic (NaCMC and CP) and more hydrophilic (HEC) polymers showed better swelling ratio, bioadhesive and mechanical characters compared with hydrogels prepared using cationic (PVP) or less hydrophilic (HPMC) polymers.					
31712156	12	77	theme	prepared	1796:1803	arg1	polysaccharide					1830:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	the prepared Egyptian Avena sativa L. polysaccharide	1792:1843	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	4	78	theme	bacitracin	568:577	arg1	zinc					579:582	0.3% bacitracin zinc	563:582	0.3% bacitracin zinc	563:582	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc were developed using the freezing-thawing method.					
31712156	3	79	theme	major	443:447	arg1	glucose					427:433	glucose	427:433	glucose	427:433	The β-glucan content was 13.28% and GC analysis revealed that glucose was the major sugar component (71.19%).					
31712156	3	79	theme	major	443:447	arg1	component					455:463	the major sugar component	439:463	the major sugar component (71.19%)	439:472	The β-glucan content was 13.28% and GC analysis revealed that glucose was the major sugar component (71.19%).					
31712156	3	79	theme	major	443:447	arg1	%					471:471	71.19%	466:471	71.19%	466:471	The β-glucan content was 13.28% and GC analysis revealed that glucose was the major sugar component (71.19%).					
31712156	0	80	theme	composite	24:32	arg1	hydrogel					34:41	Efficient wound healing composite hydrogel	0:41	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide	0:87	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide containing β-glucan.					
31712156	5	81	theme	Na	807:808	arg1	carboxymethylcellulose					783:804	sodium carboxymethylcellulose	776:804	sodium carboxymethylcellulose (Na CMC)	776:813	The used polymers were; polyvinylpyrrolidone (PVP), Carbopol 940 (CP), hydroxyethylcellulose (HEC), hydroxypropyl methylcellulose (HPMC), and sodium carboxymethylcellulose (Na CMC).					
31712156	5	81	theme	Na	807:808	arg1	CMC					810:812	Na CMC	807:812	Na CMC	807:812	The used polymers were; polyvinylpyrrolidone (PVP), Carbopol 940 (CP), hydroxyethylcellulose (HEC), hydroxypropyl methylcellulose (HPMC), and sodium carboxymethylcellulose (Na CMC).					
31712156	0	82	dep	Avena	58:62	arg1	L.					71:72	Avena sativa L.	58:72	Egyptian Avena sativa L. polysaccharide	49:87	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide containing β-glucan.					
31712156	0	83	theme	Avena	58:62	arg1	polysaccharide					74:87	Egyptian Avena sativa L. polysaccharide	49:87	Egyptian Avena sativa L. polysaccharide	49:87	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide containing β-glucan.					
31712156	4	84	theme	0.3	563:565	arg1	%					566:566	%	566:566	%	566:566	Different PVA-polysaccharide hydrogels combined with different polymers and loaded with 0.3% bacitracin zinc were developed using the freezing-thawing method.					
31712156	12	85	theme	current	1746:1752	arg1	study					1754:1758	The current study	1742:1758	The current study	1742:1758	The current study revealed the potential of using the prepared Egyptian Avena sativa L. polysaccharide and HEC for development of an efficient wound healing dressing with antimicrobial and anti-inflammatory activities.					
31712156	6	86	theme	prepared	820:827	arg1	hydrogels					829:837	The prepared hydrogels	816:837	The prepared hydrogels	816:837	The prepared hydrogels were characterized by determination of gel fraction, swelling ratio, mechanical and bioadhesive properties.					
31712156	9	87	dep	anti-inflammatory	1425:1441	arg1	activity					1461:1468	activity	1461:1468	activity	1461:1468	In addition, In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products were performed on rats.					
31712156	9	87	dep	anti-inflammatory	1425:1441	arg1	the					1421:1423	the	1421:1423	the	1421:1423	In addition, In vivo evaluation of the anti-inflammatory and wound healing activity compared with conventional products were performed on rats.					
31712156	10	88	theme	%	1635:1635	arg1	reduction					1643:1651	19.8% edema reduction	1631:1651	19.8% edema reduction	1631:1651	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	10	88	theme	%	1635:1635	arg1	F9					1627:1628	F9	1627:1628	F9 (19.8% edema reduction)	1627:1652	The results showed higher anti-inflammatory activity of F7 (21.4% edema reduction) compared with F9 (19.8% edema reduction).					
31712156	0	89	theme	wound	10:14	arg1	healing					16:22	Efficient wound healing	0:22	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide	0:87	Efficient wound healing composite hydrogel using Egyptian Avena sativa L. polysaccharide containing β-glucan.					
31887375	2	0	theme	structural	344:353	arg1	properties					400:409	the structural, mechanical, biodegradable and antimicrobial properties	340:409	the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films	340:425	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	5	1	with	overloading	850:860	arg1	DL					867:868	DL	867:868	DL	867:868	However, the overloading with DL may weaken hydrogen bonds between polymer chains, resulting in the negative effects on the physical performances of the film.					
31887375	6	2	theme	mango	1148:1152	arg1	fruits					1154:1159	packaged mango fruits	1139:1159	packaged mango fruits	1139:1159	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	7	3	theme	packaging	1402:1410	arg1	material					1412:1419	a promising eco-friendly packaging material	1377:1419	a promising eco-friendly packaging material for food preservation	1377:1441	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	7	3	theme	packaging	1402:1410	arg1	films					1364:1368	DL/PVA/CS composite films	1344:1368	DL/PVA/CS composite films	1344:1368	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	2	4	theme	PVA/CS	414:419	arg1	films					421:425	PVA/CS films	414:425	PVA/CS films	414:425	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	5	5	theme	film	990:993	arg1	performances					970:981	the physical performances	957:981	the physical performances of the film	957:993	However, the overloading with DL may weaken hydrogen bonds between polymer chains, resulting in the negative effects on the physical performances of the film.					
31887375	6	6	theme	packaged	1139:1146	arg1	fruits					1154:1159	packaged mango fruits	1139:1159	packaged mango fruits	1139:1159	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	1	7	theme	Active	146:151	arg1	films					177:181	Active biodegradable packaging films	146:181	Active biodegradable packaging films	146:181	Active biodegradable packaging films were developed with polyvinyl alcohol (PVA), chitosan (CS) and d-Limonene (DL).					
31887375	2	8	from	effect	267:272	arg1	properties					400:409	the structural, mechanical, biodegradable and antimicrobial properties	340:409	the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films	340:425	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	2	9	theme	antimicrobial	386:398	arg1	properties					400:409	the structural, mechanical, biodegradable and antimicrobial properties	340:409	the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films	340:425	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	4	10	theme	antibacterial	688:700	arg1	properties					726:735	their antibacterial, mechanical and barrier properties	682:735	properties	726:735	PVA/CS films greatly improved their antibacterial, mechanical and barrier properties, which are associated with the considerable biodegradability, after they were incorporated with DL.					
31887375	7	11	theme	promising	1379:1387	arg1	material					1412:1419	a promising eco-friendly packaging material	1377:1419	a promising eco-friendly packaging material for food preservation	1377:1441	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	7	11	theme	promising	1379:1387	arg1	films					1364:1368	DL/PVA/CS composite films	1344:1368	DL/PVA/CS composite films	1344:1368	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	5	12	from	effects	946:952	arg1	performances					970:981	the physical performances	957:981	the physical performances of the film	957:993	However, the overloading with DL may weaken hydrogen bonds between polymer chains, resulting in the negative effects on the physical performances of the film.					
31887375	6	13	dep	fruits	1233:1238	arg1	rate					1259:1262	decay rate	1253:1262	decay rate	1253:1262	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	13	dep	fruits	1233:1238	arg1	solids					1303:1308	soluble solids	1295:1308	soluble solids	1295:1308	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	13	dep	fruits	1233:1238	arg1	fruits					1233:1238	fruits	1233:1238	fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid	1233:1327	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	13	dep	fruits	1233:1238	arg1	firmness					1265:1272	firmness	1265:1272	firmness	1265:1272	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	13	dep	fruits	1233:1238	arg1	loss					1247:1250	weight loss	1240:1250	weight loss	1240:1250	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	13	dep	fruits	1233:1238	arg1	acidity					1286:1292	titratable acidity	1275:1292	titratable acidity	1275:1292	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	13	dep	fruits	1233:1238	arg1	acid					1324:1327	ascorbic acid	1315:1327	ascorbic acid	1315:1327	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	2	14	dep	levels	296:301	arg1	%					311:311	2.5%	308:311	2.5%	308:311	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	2	14	dep	levels	296:301	arg1	%					315:315	5%	314:315	5%	314:315	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	2	14	dep	levels	296:301	arg1	%					321:321	7.5%	318:321	7.5%	318:321	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	2	14	dep	levels	296:301	arg1	%					330:330	10% w/w	328:334	10% w/w	328:334	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	2	14	dep	levels	296:301	arg1	%					305:305	0%	304:305	0%	304:305	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	7	15	theme	eco-friendly	1389:1400	arg1	material					1412:1419	a promising eco-friendly packaging material	1377:1419	a promising eco-friendly packaging material for food preservation	1377:1441	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	7	15	theme	eco-friendly	1389:1400	arg1	films					1364:1368	DL/PVA/CS composite films	1344:1368	DL/PVA/CS composite films	1344:1368	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	2	16	theme	biodegradable	368:380	arg1	properties					400:409	the structural, mechanical, biodegradable and antimicrobial properties	340:409	the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films	340:425	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	4	17	theme	mechanical	703:712	arg1	properties					726:735	their antibacterial, mechanical and barrier properties	682:735	properties	726:735	PVA/CS films greatly improved their antibacterial, mechanical and barrier properties, which are associated with the considerable biodegradability, after they were incorporated with DL.					
31887375	1	18	theme	biodegradable	153:165	arg1	films					177:181	Active biodegradable packaging films	146:181	Active biodegradable packaging films	146:181	Active biodegradable packaging films were developed with polyvinyl alcohol (PVA), chitosan (CS) and d-Limonene (DL).					
31887375	0	19	theme	structural	84:93	arg1	properties					134:143	structural, antibacterial, and fruit preservation properties	84:143	structural, antibacterial, and fruit preservation properties	84:143	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	5	20	theme	physical	961:968	arg1	performances					970:981	the physical performances	957:981	the physical performances of the film	957:993	However, the overloading with DL may weaken hydrogen bonds between polymer chains, resulting in the negative effects on the physical performances of the film.					
31887375	6	21	from	20 ± 2 °C	1189:1197	arg1	10 day					1168:1173	10 day	1168:1173	10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid	1168:1327	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	22	theme	titratable	1275:1284	arg1	fruits					1233:1238	fruits	1233:1238	fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid	1233:1327	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	22	theme	titratable	1275:1284	arg1	acidity					1286:1292	titratable acidity	1275:1292	titratable acidity	1275:1292	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	1	23	theme	packaging	167:175	arg1	films					177:181	Active biodegradable packaging films	146:181	Active biodegradable packaging films	146:181	Active biodegradable packaging films were developed with polyvinyl alcohol (PVA), chitosan (CS) and d-Limonene (DL).					
31887375	0	24	theme	poly	11:14	arg1	films					40:44	Developing poly(vinyl alcohol)/chitosan films	0:44	Developing poly(vinyl alcohol)/chitosan films	0:44	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	3	25	theme	infrared	583:590	arg1	spectroscopy					599:610	infrared (FTIR) spectroscopy	583:610	infrared (FTIR) spectroscopy	583:610	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	4	26	theme	barrier	718:724	arg1	properties					726:735	their antibacterial, mechanical and barrier properties	682:735	properties	726:735	PVA/CS films greatly improved their antibacterial, mechanical and barrier properties, which are associated with the considerable biodegradability, after they were incorporated with DL.					
31887375	6	27	theme	PVA/CS/DL-5	1014:1024	arg1	%					1025:1025	%	1025:1025	%	1025:1025	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	28	theme	decay	1253:1257	arg1	rate					1259:1262	decay rate	1253:1262	decay rate	1253:1262	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	28	theme	decay	1253:1257	arg1	fruits					1233:1238	fruits	1233:1238	fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid	1233:1327	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	0	29	theme	Developing	0:9	arg1	poly					11:14	Developing poly	0:14	Developing poly(vinyl alcohol)/chitosan films	0:44	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	0	29	theme	Developing	0:9	arg1	alcohol					22:28	vinyl alcohol	16:28	vinyl alcohol	16:28	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	6	30	theme	effective	1112:1120	arg1	preservations					1122:1134	effective preservations	1112:1134	effective preservations of packaged mango fruits	1112:1159	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	0	31	dep	d-limonene	63:72	arg1	Study					75:79	Study	75:79	d-limonene: Study of structural, antibacterial, and fruit preservation properties	63:143	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	0	32	theme	vinyl	16:20	arg1	poly					11:14	Developing poly	0:14	Developing poly(vinyl alcohol)/chitosan films	0:44	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	0	32	theme	vinyl	16:20	arg1	alcohol					22:28	vinyl alcohol	16:28	vinyl alcohol	16:28	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	3	33	theme	scanning	616:623	arg1	microscopy					634:643	scanning electron microscopy	616:643	scanning electron microscopy (SEM)	616:649	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	3	33	theme	scanning	616:623	arg1	SEM					646:648	SEM	646:648	SEM	646:648	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	2	34	theme	films	421:425	arg1	properties					400:409	the structural, mechanical, biodegradable and antimicrobial properties	340:409	the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films	340:425	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	5	35	theme	polymer	904:910	arg1	chains					912:917	polymer chains	904:917	polymer chains	904:917	However, the overloading with DL may weaken hydrogen bonds between polymer chains, resulting in the negative effects on the physical performances of the film.					
31887375	3	36	from	compatibility	460:472	arg1	matrix					537:542	the PVA/CS matrix	526:542	the PVA/CS matrix	526:542	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	0	37	theme	antibacterial	96:108	arg1	properties					134:143	structural, antibacterial, and fruit preservation properties	84:143	structural, antibacterial, and fruit preservation properties	84:143	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	3	38	theme	of	517:518	arg1	DL					520:521	good dispersion of DL	501:521	good dispersion of DL	501:521	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	2	39	theme	levels	296:301	arg1	effect					267:272	The effect	263:272	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films	263:425	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	3	40	dep	Fourier	565:571	arg1	transform					573:581	transform	573:581	transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM)	573:649	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	0	41	theme	fruit	115:119	arg1	properties					134:143	structural, antibacterial, and fruit preservation properties	84:143	structural, antibacterial, and fruit preservation properties	84:143	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	6	42	theme	soluble	1295:1301	arg1	solids					1303:1308	soluble solids	1295:1308	soluble solids	1295:1308	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	1	43	theme	polyvinyl	203:211	arg1	PVA					222:224	PVA	222:224	PVA	222:224	Active biodegradable packaging films were developed with polyvinyl alcohol (PVA), chitosan (CS) and d-Limonene (DL).					
31887375	1	43	theme	polyvinyl	203:211	arg1	alcohol					213:219	polyvinyl alcohol	203:219	polyvinyl alcohol (PVA)	203:225	Active biodegradable packaging films were developed with polyvinyl alcohol (PVA), chitosan (CS) and d-Limonene (DL).					
31887375	6	44	theme	storage	1178:1184	arg1	10 day					1168:1173	10 day	1168:1173	10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid	1168:1327	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	3	45	theme	good	501:504	arg1	DL					520:521	good dispersion of DL	501:521	good dispersion of DL	501:521	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	5	46	theme	hydrogen	881:888	arg1	bonds					890:894	hydrogen bonds	881:894	hydrogen bonds between polymer chains	881:917	However, the overloading with DL may weaken hydrogen bonds between polymer chains, resulting in the negative effects on the physical performances of the film.					
31887375	2	47	theme	w/w	332:334	arg1	%					330:330	10% w/w	328:334	10% w/w	328:334	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	3	48	dep	infrared	583:590	arg1	FTIR					593:596	FTIR	593:596	FTIR	593:596	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	0	49	theme	properties	134:143	arg1	Study					75:79	Study	75:79	d-limonene: Study of structural, antibacterial, and fruit preservation properties	63:143	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	3	50	theme	dispersion	506:515	arg1	DL					520:521	good dispersion of DL	501:521	good dispersion of DL	501:521	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	3	51	theme	Good	455:458	arg1	compatibility					460:472	Good compatibility	455:472	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix	455:542	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	2	52	theme	content	288:294	arg1	levels					296:301	various DL content levels	277:301	various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w)	277:335	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	0	53	theme	/chitosan	30:38	arg1	films					40:44	Developing poly(vinyl alcohol)/chitosan films	0:44	Developing poly(vinyl alcohol)/chitosan films	0:44	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	3	54	theme	electron	625:632	arg1	microscopy					634:643	scanning electron microscopy	616:643	scanning electron microscopy (SEM)	616:649	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	3	54	theme	electron	625:632	arg1	SEM					646:648	SEM	646:648	SEM	646:648	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	0	55	theme	preservation	121:132	arg1	properties					134:143	structural, antibacterial, and fruit preservation properties	84:143	structural, antibacterial, and fruit preservation properties	84:143	Developing poly(vinyl alcohol)/chitosan films incorporate with d-limonene: Study of structural, antibacterial, and fruit preservation properties.					
31887375	3	56	theme	PVA/CS	530:535	arg1	matrix					537:542	the PVA/CS matrix	526:542	the PVA/CS matrix	526:542	Good compatibility between DL and PVA/CS, and good dispersion of DL in the PVA/CS matrix were demonstrated by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM).					
31887375	2	57	theme	DL	285:286	arg1	levels					296:301	various DL content levels	277:301	various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w)	277:335	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	6	58	theme	highest	1043:1049	arg1	angle					1065:1069	water contact angle	1051:1069	water contact angle	1051:1069	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	4	59	theme	considerable	768:779	arg1	biodegradability					781:796	the considerable biodegradability	764:796	the considerable biodegradability	764:796	PVA/CS films greatly improved their antibacterial, mechanical and barrier properties, which are associated with the considerable biodegradability, after they were incorporated with DL.					
31887375	7	60	theme	composite	1354:1362	arg1	material					1412:1419	a promising eco-friendly packaging material	1377:1419	a promising eco-friendly packaging material for food preservation	1377:1441	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	7	60	theme	composite	1354:1362	arg1	films					1364:1368	DL/PVA/CS composite films	1344:1368	DL/PVA/CS composite films	1344:1368	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	7	61	theme	DL/PVA/CS	1344:1352	arg1	material					1412:1419	a promising eco-friendly packaging material	1377:1419	a promising eco-friendly packaging material for food preservation	1377:1441	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	7	61	theme	DL/PVA/CS	1344:1352	arg1	films					1364:1368	DL/PVA/CS composite films	1344:1368	DL/PVA/CS composite films	1344:1368	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31887375	5	62	theme	negative	937:944	arg1	effects					946:952	the negative effects	933:952	the negative effects on the physical performances of the film	933:993	However, the overloading with DL may weaken hydrogen bonds between polymer chains, resulting in the negative effects on the physical performances of the film.					
31887375	2	63	theme	various	277:283	arg1	levels					296:301	various DL content levels	277:301	various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w)	277:335	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	6	64	theme	fruits	1154:1159	arg1	preservations					1122:1134	effective preservations	1112:1134	effective preservations of packaged mango fruits	1112:1159	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	65	theme	transmittance	1075:1087	arg1	value					1089:1093	transmittance value	1075:1093	transmittance value	1075:1093	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	66	theme	contact	1057:1063	arg1	angle					1065:1069	water contact angle	1051:1069	water contact angle	1051:1069	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	67	theme	ascorbic	1315:1322	arg1	fruits					1233:1238	fruits	1233:1238	fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid	1233:1327	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	67	theme	ascorbic	1315:1322	arg1	acid					1324:1327	ascorbic acid	1315:1327	ascorbic acid	1315:1327	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	68	dep	angle	1065:1069	arg1	the					1039:1041	the	1039:1041	the	1039:1041	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	2	69	theme	mechanical	356:365	arg1	properties					400:409	the structural, mechanical, biodegradable and antimicrobial properties	340:409	the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films	340:425	The effect of various DL content levels (0%, 2.5%, 5%, 7.5%, and 10% w/w) on the structural, mechanical, biodegradable and antimicrobial properties of PVA/CS films was systematically studied.					
31887375	6	70	theme	water	1051:1055	arg1	angle					1065:1069	water contact angle	1051:1069	water contact angle	1051:1069	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	71	theme	%	1025:1025	arg1	film					1027:1030	the PVA/CS/DL-5% film	1010:1030	the PVA/CS/DL-5% film	1010:1030	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	72	theme	weight	1240:1245	arg1	fruits					1233:1238	fruits	1233:1238	fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid	1233:1327	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	6	72	theme	weight	1240:1245	arg1	loss					1247:1250	weight loss	1240:1250	weight loss	1240:1250	Particularly, the PVA/CS/DL-5% film showed the highest water contact angle and transmittance value, and also showed effective preservations of packaged mango fruits during 10 day of storage at 20 ± 2 °C, based on the characterization by fruits weight loss, decay rate, firmness, titratable acidity, soluble solids, and ascorbic acid.					
31887375	4	73	theme	PVA/CS	652:657	arg1	films					659:663	PVA/CS films	652:663	PVA/CS films	652:663	PVA/CS films greatly improved their antibacterial, mechanical and barrier properties, which are associated with the considerable biodegradability, after they were incorporated with DL.					
31887375	7	74	theme	food	1425:1428	arg1	preservation					1430:1441	food preservation	1425:1441	food preservation	1425:1441	Consequently, DL/PVA/CS composite films may be a promising eco-friendly packaging material for food preservation.					
31007094	0	0	theme	tendon-bone	87:97	arg1	repair					108:113	tendon-bone junction repair	87:113	tendon-bone junction repair	87:113	The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair.					
31007094	5	1	theme	divergent	815:823	arg1	content					833:839	divergent mineral content	815:839	divergent mineral content	815:839	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	6	2	theme	strain	1120:1125	arg1	profiles					1127:1134	non-uniform strain profiles	1108:1134	non-uniform strain profiles across the spatially-graded scaffold	1108:1171	Results: Here, we report application of cyclic tensile strain (CTS) to the scaffold via a bioreactor leads to non-uniform strain profiles across the spatially-graded scaffold.					
31007094	4	3	theme	spatially-graded	600:615	arg1	matrix					631:636	spatially-graded extracellular matrix	600:636	spatially-graded extracellular matrix	600:636	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	3	4	theme	TBJ	314:316	arg1	injuries					318:325	TBJ injuries	314:325	TBJ injuries	314:325	Current clinical strategies for TBJ injuries prioritize mechanical reattachment over regeneration of the native interface, resulting in poor outcomes.					
31007094	0	5	from	influence	4:12	arg1	collagen-GAG					60:71	multi-compartment collagen-GAG	42:71	multi-compartment collagen-GAG	42:71	The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair.					
31007094	9	6	theme	tensile	1611:1617	arg1	stimuli					1619:1625	cyclic tensile stimuli	1604:1625	cyclic tensile stimuli	1604:1625	This work demonstrates a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces.					
31007094	5	7	theme	compositional	956:968	arg1	features					970:977	structural and compositional features	941:977	structural and compositional features of the native TBJ	941:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	6	8	theme	cyclic	1038:1043	arg1	CTS					1061:1063	CTS	1061:1063	CTS	1061:1063	Results: Here, we report application of cyclic tensile strain (CTS) to the scaffold via a bioreactor leads to non-uniform strain profiles across the spatially-graded scaffold.					
31007094	6	8	theme	cyclic	1038:1043	arg1	strain					1053:1058	cyclic tensile strain	1038:1058	cyclic tensile strain (CTS)	1038:1064	Results: Here, we report application of cyclic tensile strain (CTS) to the scaffold via a bioreactor leads to non-uniform strain profiles across the spatially-graded scaffold.					
31007094	7	9	theme	CTS	1199:1201	arg1	combinations					1183:1194	combinations	1183:1194	combinations of CTS and matrix structural features	1183:1232	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	6	10	dep	bioreactor	1088:1097	arg1	leads					1099:1103	leads	1099:1103	leads to non-uniform strain profiles across the spatially-graded scaffold	1099:1171	Results: Here, we report application of cyclic tensile strain (CTS) to the scaffold via a bioreactor leads to non-uniform strain profiles across the spatially-graded scaffold.					
31007094	8	11	theme	MSC	1422:1424	arg1	activity					1426:1433	MSC activity	1422:1433	MSC activity	1422:1433	CTS preferentially upregulates MSC activity and tenogenic differentiation in the anisotropic region of the scaffold.					
31007094	2	12	theme	interface	271:279	arg1	junction					224:231	The tendon-bone junction	208:231	The tendon-bone junction (TBJ)	208:237	The tendon-bone junction (TBJ) is a classic example of such an interface.					
31007094	2	12	theme	interface	271:279	arg1	example					252:258	a classic example	242:258	a classic example of such an interface	242:279	The tendon-bone junction (TBJ) is a classic example of such an interface.					
31007094	4	13	theme	matrix	631:636	arg1	features					584:591	features	584:591	features of the spatially-graded extracellular matrix and strain profiles across the native TBJ	584:678	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	0	14	theme	junction	99:106	arg1	repair					108:113	tendon-bone junction repair	87:113	tendon-bone junction repair	87:113	The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair.					
31007094	4	15	theme	spatially-graded	477:492	arg1	tissues					494:500	spatially-graded tissues	477:500	spatially-graded tissues	477:500	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	7	16	dep	cells	1339:1343	arg1	lineages					1381:1388	multiple osteotendinous lineages	1357:1388	multiple osteotendinous lineages	1357:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	17	theme	bone	1302:1305	arg1	cells					1339:1343	human bone marrow-derived mesenchymal stem cells	1296:1343	human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages	1296:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	17	theme	bone	1302:1305	arg1	MSCs					1346:1349	MSCs	1346:1349	MSCs	1346:1349	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	18	theme	human	1296:1300	arg1	cells					1339:1343	human bone marrow-derived mesenchymal stem cells	1296:1343	human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages	1296:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	18	theme	human	1296:1300	arg1	MSCs					1346:1349	MSCs	1346:1349	MSCs	1346:1349	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	4	19	theme	extracellular	617:629	arg1	matrix					631:636	spatially-graded extracellular matrix	600:636	spatially-graded extracellular matrix	600:636	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	3	20	theme	mechanical	338:347	arg1	reattachment					349:360	mechanical reattachment	338:360	mechanical reattachment	338:360	Current clinical strategies for TBJ injuries prioritize mechanical reattachment over regeneration of the native interface, resulting in poor outcomes.					
31007094	5	21	contain	containing	778:787	arg1	scaffold					769:776	a biphasic collagen-glycosaminoglycan (CG) scaffold	726:776	a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ	726:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	5	21	contain	containing	778:787	arg2	compartment					798:808	distinct compartment	789:808	distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ	789:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	9	22	theme	orthopedic	1662:1671	arg1	interfaces					1673:1682	orthopedic interfaces	1662:1682	orthopedic interfaces	1662:1682	This work demonstrates a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces.					
31007094	5	23	theme	continuous	906:915	arg1	zone					927:930	a continuous interface zone	904:930	a continuous interface zone to mimic structural and compositional features of the native TBJ	904:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	5	24	theme	distinct	789:796	arg1	compartment					798:808	distinct compartment	789:808	distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ	789:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	5	25	dep	alignment	856:864	arg1	isotropic					867:875	isotropic	867:875	isotropic	867:875	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	5	25	dep	alignment	856:864	arg1	anisotropic					881:891	anisotropic	881:891	anisotropic	881:891	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	7	26	theme	rapid	1242:1246	arg1	profiles					1284:1291	rapid, spatially-distinct differentiation profiles	1242:1291	rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages	1242:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	1	27	theme	soft	185:188	arg1	tissues					190:196	soft tissues	185:196	soft tissues	185:196	Background: Orthopedic injuries often occur at the interface between soft tissues and bone.					
31007094	3	28	theme	native	387:392	arg1	interface					394:402	the native interface	383:402	the native interface	383:402	Current clinical strategies for TBJ injuries prioritize mechanical reattachment over regeneration of the native interface, resulting in poor outcomes.					
31007094	4	29	theme	tissues	494:500	arg1	healing					466:472	regenerative healing	453:472	regenerative healing of spatially-graded tissues	453:500	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	7	30	theme	differentiation	1268:1282	arg1	profiles					1284:1291	rapid, spatially-distinct differentiation profiles	1242:1291	rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages	1242:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	31	theme	mesenchymal	1322:1332	arg1	cells					1339:1343	human bone marrow-derived mesenchymal stem cells	1296:1343	human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages	1296:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	31	theme	mesenchymal	1322:1332	arg1	MSCs					1346:1349	MSCs	1346:1349	MSCs	1346:1349	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	5	32	theme	TBJ	993:995	arg1	features					970:977	structural and compositional features	941:977	structural and compositional features of the native TBJ	941:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	7	33	theme	cells	1339:1343	arg1	profiles					1284:1291	rapid, spatially-distinct differentiation profiles	1242:1291	rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages	1242:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	9	34	theme	tissue	1533:1538	arg1	approach					1552:1559	a tissue engineering approach	1531:1559	a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces	1531:1682	This work demonstrates a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces.					
31007094	3	35	theme	interface	394:402	arg1	regeneration					367:378	regeneration	367:378	regeneration of the native interface	367:402	Current clinical strategies for TBJ injuries prioritize mechanical reattachment over regeneration of the native interface, resulting in poor outcomes.					
31007094	8	36	theme	tenogenic	1439:1447	arg1	differentiation					1449:1463	tenogenic differentiation	1439:1463	tenogenic differentiation	1439:1463	CTS preferentially upregulates MSC activity and tenogenic differentiation in the anisotropic region of the scaffold.					
31007094	6	37	theme	tensile	1045:1051	arg1	CTS					1061:1063	CTS	1061:1063	CTS	1061:1063	Results: Here, we report application of cyclic tensile strain (CTS) to the scaffold via a bioreactor leads to non-uniform strain profiles across the spatially-graded scaffold.					
31007094	6	37	theme	tensile	1045:1051	arg1	strain					1053:1058	cyclic tensile strain	1038:1058	cyclic tensile strain (CTS)	1038:1064	Results: Here, we report application of cyclic tensile strain (CTS) to the scaffold via a bioreactor leads to non-uniform strain profiles across the spatially-graded scaffold.					
31007094	9	38	theme	engineering	1540:1550	arg1	approach					1552:1559	a tissue engineering approach	1531:1559	a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces	1531:1682	This work demonstrates a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces.					
31007094	0	39	theme	cyclic	17:22	arg1	strain					32:37	cyclic tensile strain	17:37	cyclic tensile strain	17:37	The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair.					
31007094	5	40	theme	biphasic	728:735	arg1	scaffold					769:776	a biphasic collagen-glycosaminoglycan (CG) scaffold	726:776	a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ	726:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	4	41	theme	native	669:674	arg1	TBJ					676:678	the native TBJ	665:678	the native TBJ	665:678	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	5	42	theme	collagen-glycosaminoglycan	737:762	arg1	scaffold					769:776	a biphasic collagen-glycosaminoglycan (CG) scaffold	726:776	a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ	726:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	4	43	dep	matrix	631:636	arg1	the					596:598	the	596:598	the	596:598	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	6	44	theme	non-uniform	1108:1118	arg1	profiles					1127:1134	non-uniform strain profiles	1108:1134	non-uniform strain profiles across the spatially-graded scaffold	1108:1171	Results: Here, we report application of cyclic tensile strain (CTS) to the scaffold via a bioreactor leads to non-uniform strain profiles across the spatially-graded scaffold.					
31007094	9	45	theme	regenerative	1638:1649	arg1	healing					1651:1657	regenerative healing	1638:1657	regenerative healing of orthopedic interfaces	1638:1682	This work demonstrates a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces.					
31007094	7	46	theme	osteotendinous	1366:1379	arg1	lineages					1381:1388	multiple osteotendinous lineages	1357:1388	multiple osteotendinous lineages	1357:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	0	47	theme	strain	32:37	arg1	influence					4:12	The influence	0:12	The influence of cyclic tensile strain on multi-compartment collagen-GAG	0:71	The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair.					
31007094	7	48	link	marrow-derived	1307:1320	arg1	cells					1339:1343	human bone marrow-derived mesenchymal stem cells	1296:1343	human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages	1296:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	48	link	marrow-derived	1307:1320	arg1	MSCs					1346:1349	MSCs	1346:1349	MSCs	1346:1349	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	49	theme	structural	1214:1223	arg1	features					1225:1232	matrix structural features	1207:1232	matrix structural features	1207:1232	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	0	50	theme	tensile	24:30	arg1	strain					32:37	cyclic tensile strain	17:37	cyclic tensile strain	17:37	The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair.					
31007094	4	51	theme	tissue	537:542	arg1	technologies					556:567	new tissue engineering technologies	533:567	new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ	533:678	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	3	52	theme	poor	418:421	arg1	outcomes					423:430	poor outcomes	418:430	poor outcomes	418:430	Current clinical strategies for TBJ injuries prioritize mechanical reattachment over regeneration of the native interface, resulting in poor outcomes.					
31007094	5	53	dep	Materials	681:689	arg1	described					716:724	described	716:724	described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ	716:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	9	54	theme	instructive	1574:1584	arg1	biomaterials					1586:1597	instructive biomaterials	1574:1597	instructive biomaterials	1574:1597	This work demonstrates a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces.					
31007094	0	55	theme	multi-compartment	42:58	arg1	collagen-GAG					60:71	multi-compartment collagen-GAG	42:71	multi-compartment collagen-GAG	42:71	The influence of cyclic tensile strain on multi-compartment collagen-GAG scaffolds for tendon-bone junction repair.					
31007094	4	56	theme	new	533:535	arg1	technologies					556:567	new tissue engineering technologies	533:567	new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ	533:678	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	3	57	theme	clinical	290:297	arg1	strategies					299:308	Current clinical strategies	282:308	Current clinical strategies for TBJ injuries	282:325	Current clinical strategies for TBJ injuries prioritize mechanical reattachment over regeneration of the native interface, resulting in poor outcomes.					
31007094	4	58	theme	regenerative	453:464	arg1	healing					466:472	regenerative healing	453:472	regenerative healing of spatially-graded tissues	453:500	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	5	59	theme	CG	765:766	arg1	scaffold					769:776	a biphasic collagen-glycosaminoglycan (CG) scaffold	726:776	a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ	726:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	9	60	theme	cyclic	1604:1609	arg1	stimuli					1619:1625	cyclic tensile stimuli	1604:1625	cyclic tensile stimuli	1604:1625	This work demonstrates a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces.					
31007094	2	61	theme	tendon-bone	212:222	arg1	TBJ					234:236	TBJ	234:236	TBJ	234:236	The tendon-bone junction (TBJ) is a classic example of such an interface.					
31007094	2	61	theme	tendon-bone	212:222	arg1	junction					224:231	The tendon-bone junction	208:231	The tendon-bone junction (TBJ)	208:237	The tendon-bone junction (TBJ) is a classic example of such an interface.					
31007094	2	61	theme	tendon-bone	212:222	arg1	example					252:258	a classic example	242:258	a classic example of such an interface	242:279	The tendon-bone junction (TBJ) is a classic example of such an interface.					
31007094	1	62	theme	Orthopedic	128:137	arg1	injuries					139:146	Orthopedic injuries	128:146	Orthopedic injuries	128:146	Background: Orthopedic injuries often occur at the interface between soft tissues and bone.					
31007094	4	63	theme	engineering	544:554	arg1	technologies					556:567	new tissue engineering technologies	533:567	new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ	533:678	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	7	64	dep	rapid	1242:1246	arg1	spatially-distinct					1249:1266	spatially-distinct	1249:1266	spatially-distinct	1249:1266	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	65	theme	matrix	1207:1212	arg1	features					1225:1232	matrix structural features	1207:1232	matrix structural features	1207:1232	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	8	66	theme	anisotropic	1472:1482	arg1	region					1484:1489	the anisotropic region	1468:1489	the anisotropic region of the scaffold	1468:1505	CTS preferentially upregulates MSC activity and tenogenic differentiation in the anisotropic region of the scaffold.					
31007094	6	67	theme	spatially-graded	1147:1162	arg1	scaffold					1164:1171	the spatially-graded scaffold	1143:1171	the spatially-graded scaffold	1143:1171	Results: Here, we report application of cyclic tensile strain (CTS) to the scaffold via a bioreactor leads to non-uniform strain profiles across the spatially-graded scaffold.					
31007094	4	68	theme	strain	642:647	arg1	profiles					649:656	strain profiles	642:656	strain profiles	642:656	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31007094	3	69	theme	Current	282:288	arg1	strategies					299:308	Current clinical strategies	282:308	Current clinical strategies for TBJ injuries	282:325	Current clinical strategies for TBJ injuries prioritize mechanical reattachment over regeneration of the native interface, resulting in poor outcomes.					
31007094	9	70	theme	interfaces	1673:1682	arg1	healing					1651:1657	regenerative healing	1638:1657	regenerative healing of orthopedic interfaces	1638:1682	This work demonstrates a tissue engineering approach that couples instructive biomaterials with cyclic tensile stimuli to promote regenerative healing of orthopedic interfaces.					
31007094	2	71	theme	classic	244:250	arg1	junction					224:231	The tendon-bone junction	208:231	The tendon-bone junction (TBJ)	208:237	The tendon-bone junction (TBJ) is a classic example of such an interface.					
31007094	2	71	theme	classic	244:250	arg1	example					252:258	a classic example	242:258	a classic example of such an interface	242:279	The tendon-bone junction (TBJ) is a classic example of such an interface.					
31007094	5	72	theme	mineral	825:831	arg1	content					833:839	divergent mineral content	815:839	divergent mineral content	815:839	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	5	73	theme	native	986:991	arg1	TBJ					993:995	the native TBJ	982:995	the native TBJ	982:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	7	74	theme	stem	1334:1337	arg1	cells					1339:1343	human bone marrow-derived mesenchymal stem cells	1296:1343	human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages	1296:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	74	theme	stem	1334:1337	arg1	MSCs					1346:1349	MSCs	1346:1349	MSCs	1346:1349	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	8	75	theme	scaffold	1498:1505	arg1	region					1484:1489	the anisotropic region	1468:1489	the anisotropic region of the scaffold	1468:1505	CTS preferentially upregulates MSC activity and tenogenic differentiation in the anisotropic region of the scaffold.					
31007094	5	76	with	compartment	798:808	arg1	content					833:839	divergent mineral content	815:839	divergent mineral content	815:839	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	5	76	with	compartment	798:808	arg1	alignment					856:864	structural alignment	845:864	structural alignment (isotropic vs. anisotropic)	845:892	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	7	77	theme	multiple	1357:1364	arg1	lineages					1381:1388	multiple osteotendinous lineages	1357:1388	multiple osteotendinous lineages	1357:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	78	theme	marrow-derived	1307:1320	arg1	cells					1339:1343	human bone marrow-derived mesenchymal stem cells	1296:1343	human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages	1296:1388	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	7	78	theme	marrow-derived	1307:1320	arg1	MSCs					1346:1349	MSCs	1346:1349	MSCs	1346:1349	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	5	79	theme	interface	917:925	arg1	zone					927:930	a continuous interface zone	904:930	a continuous interface zone to mimic structural and compositional features of the native TBJ	904:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	5	80	theme	structural	845:854	arg1	alignment					856:864	structural alignment	845:864	structural alignment (isotropic vs. anisotropic)	845:892	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	7	81	theme	features	1225:1232	arg1	combinations					1183:1194	combinations	1183:1194	combinations of CTS and matrix structural features	1183:1232	Further, combinations of CTS and matrix structural features promote rapid, spatially-distinct differentiation profiles of human bone marrow-derived mesenchymal stem cells (MSCs) down multiple osteotendinous lineages.					
31007094	6	82	theme	strain	1053:1058	arg1	application					1023:1033	application	1023:1033	application of cyclic tensile strain (CTS) to the scaffold	1023:1080	Results: Here, we report application of cyclic tensile strain (CTS) to the scaffold via a bioreactor leads to non-uniform strain profiles across the spatially-graded scaffold.					
31007094	5	83	theme	structural	941:950	arg1	features					970:977	structural and compositional features	941:977	structural and compositional features of the native TBJ	941:995	Materials and Methods: We recently described a biphasic collagen-glycosaminoglycan (CG) scaffold containing distinct compartment with divergent mineral content and structural alignment (isotropic vs. anisotropic) linked by a continuous interface zone to mimic structural and compositional features of the native TBJ.					
31007094	4	84	theme	profiles	649:656	arg1	features					584:591	features	584:591	features of the spatially-graded extracellular matrix and strain profiles across the native TBJ	584:678	The need to promote regenerative healing of spatially-graded tissues inspires our effort to develop new tissue engineering technologies that replicate features of the spatially-graded extracellular matrix and strain profiles across the native TBJ.					
31334644	2	0	from	oligosaccharides	438:453	arg1	milk					466:469	milk	466:469	milk	466:469	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	6	1	theme	activity	1290:1297	arg1	characterization					1252:1267	further characterization	1244:1267	further characterization of the structure and activity of HMOs	1244:1305	These findings provide the groundwork for further characterization of the structure and activity of HMOs.					
31334644	5	2	link	α-2,6-linked	1082:1093	arg1	acid					1106:1109	α-2,6-linked monosialic acid	1082:1109	α-2,6-linked monosialic acid	1082:1109	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	5	3	theme	disialic	1187:1194	arg1	acid					1196:1199	both α-2,3- and α-2,6-linked disialic acid	1158:1199	both α-2,3- and α-2,6-linked disialic acid	1158:1199	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	0	4	theme	Neutral	100:106	arg1	Oligosaccharides					128:143	Human Milk Neutral and Sialylated Free Oligosaccharides	89:143	Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization	89:193	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	5	5	theme	disialic	1132:1139	arg1	acid					1141:1144	α-2,6-linked disialic acid	1119:1144	α-2,6-linked disialic acid	1119:1144	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	2	6	gly	sialylated	427:436	arg1	SHMOs					456:460	SHMOs	456:460	SHMOs	456:460	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	2	6	gly	sialylated	427:436	arg1	oligosaccharides					438:453	sialylated oligosaccharides	427:453	sialylated oligosaccharides (SHMOs) in milk	427:469	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	0	7	theme	Free	123:126	arg1	Oligosaccharides					128:143	Human Milk Neutral and Sialylated Free Oligosaccharides	89:143	Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization	89:193	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	6	8	theme	structure	1276:1284	arg1	characterization					1252:1267	further characterization	1244:1267	further characterization of the structure and activity of HMOs	1244:1305	These findings provide the groundwork for further characterization of the structure and activity of HMOs.					
31334644	4	9	theme	sialic	859:864	arg1	acid					866:869	selective sialic acid	849:869	selective sialic acid derivatization	849:884	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	10	theme	time-of-flight	690:703	arg1	MALDI-TOF-MS					724:735	MALDI-TOF-MS	724:735	MALDI-TOF-MS	724:735	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	10	theme	time-of-flight	690:703	arg1	spectrometry					710:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	646:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	646:736	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	0	11	theme	Sialylated	112:121	arg1	Oligosaccharides					128:143	Human Milk Neutral and Sialylated Free Oligosaccharides	89:143	Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization	89:193	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	5	12	theme	monosialic	1095:1104	arg1	acid					1106:1109	α-2,6-linked monosialic acid	1082:1109	α-2,6-linked monosialic acid	1082:1109	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	5	13	theme	SHMOs	986:990	arg1	SHMOs					986:990	28 SHMOs	983:990	28 SHMOs	983:990	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	5	13	theme	SHMOs	986:990	arg1	8					1002:1002	8	1002:1002	8	1002:1002	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	5	14	theme	Sixty-four	896:905	arg1	oligosaccharides					907:922	Sixty-four oligosaccharides	896:922	Sixty-four oligosaccharides	896:922	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	3	15	theme	anion	521:525	arg1	chromatography					527:540	DEAE-52 anion chromatography	513:540	DEAE-52 anion chromatography	513:540	Here, NHMOs were separated from SHMOs by DEAE-52 anion chromatography, and lactose was removed by graphite carbon solid-phase extraction.					
31334644	1	16	theme	functional	200:209	arg1	role					211:214	The functional role	196:214	The functional role of human milk oligosaccharides (HMOs)	196:252	The functional role of human milk oligosaccharides (HMOs) is closely associated with their type, composition, and structure.					
31334644	0	17	theme	Oligosaccharides	128:143	arg1	Analysis					77:84	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis	0:84	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.	0:194	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	4	18	theme	acid	866:869	arg1	derivatization					871:884	selective sialic acid derivatization	849:884	selective sialic acid derivatization	849:884	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	5	19	link	α-2,3-linked	1009:1020	arg1	acid					1033:1036	α-2,3-linked monosialic acid	1009:1036	α-2,3-linked monosialic acid	1009:1036	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	4	20	theme	desorption/ionization	668:688	arg1	MALDI-TOF-MS					724:735	MALDI-TOF-MS	724:735	MALDI-TOF-MS	724:735	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	20	theme	desorption/ionization	668:688	arg1	spectrometry					710:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	646:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	646:736	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	0	21	theme	Laser	16:20	arg1	Spectrometry					64:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry	0:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.	0:194	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	6	22	theme	further	1244:1250	arg1	characterization					1252:1267	further characterization	1244:1267	further characterization of the structure and activity of HMOs	1244:1305	These findings provide the groundwork for further characterization of the structure and activity of HMOs.					
31334644	4	23	theme	on-target	766:774	arg1	derivatization					776:789	reagent P on-target derivatization	756:789	Girard's reagent P on-target derivatization (GPOD)	747:796	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	23	theme	on-target	766:774	arg1	GPOD					792:795	GPOD	792:795	GPOD	792:795	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	0	24	theme	Matrix-Assisted	0:14	arg1	Spectrometry					64:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry	0:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.	0:194	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	4	25	theme	P	764:764	arg1	derivatization					776:789	reagent P on-target derivatization	756:789	Girard's reagent P on-target derivatization (GPOD)	747:796	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	25	theme	P	764:764	arg1	GPOD					792:795	GPOD	792:795	GPOD	792:795	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	1	26	theme	human	219:223	arg1	oligosaccharides					230:245	human milk oligosaccharides	219:245	human milk oligosaccharides (HMOs)	219:252	The functional role of human milk oligosaccharides (HMOs) is closely associated with their type, composition, and structure.					
31334644	1	26	theme	human	219:223	arg1	HMOs					248:251	HMOs	248:251	HMOs	248:251	The functional role of human milk oligosaccharides (HMOs) is closely associated with their type, composition, and structure.					
31334644	0	27	theme	Time-of-Flight	44:57	arg1	Spectrometry					64:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry	0:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.	0:194	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	5	28	theme	α-2,3-	1163:1168	arg1	acid					1196:1199	both α-2,3- and α-2,6-linked disialic acid	1158:1199	both α-2,3- and α-2,6-linked disialic acid	1158:1199	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	6	29	dep	structure	1276:1284	arg1	the					1272:1274	the	1272:1274	the	1272:1274	These findings provide the groundwork for further characterization of the structure and activity of HMOs.					
31334644	4	30	theme	selective	849:857	arg1	acid					866:869	selective sialic acid	849:869	selective sialic acid derivatization	849:884	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	31	dep	Girard	747:752	arg1	derivatization					776:789	reagent P on-target derivatization	756:789	Girard's reagent P on-target derivatization (GPOD)	747:796	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	31	dep	Girard	747:752	arg1	GPOD					792:795	GPOD	792:795	GPOD	792:795	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	5	32	theme	monosialic	1022:1031	arg1	acid					1033:1036	α-2,3-linked monosialic acid	1009:1036	α-2,3-linked monosialic acid	1009:1036	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	1	33	theme	milk	225:228	arg1	oligosaccharides					230:245	human milk oligosaccharides	219:245	human milk oligosaccharides (HMOs)	219:252	The functional role of human milk oligosaccharides (HMOs) is closely associated with their type, composition, and structure.					
31334644	1	33	theme	milk	225:228	arg1	HMOs					248:251	HMOs	248:251	HMOs	248:251	The functional role of human milk oligosaccharides (HMOs) is closely associated with their type, composition, and structure.					
31334644	0	34	theme	Desorption/Ionization	22:42	arg1	Spectrometry					64:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry	0:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.	0:194	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	5	35	theme	α-2,6-linked	1119:1130	arg1	acid					1141:1144	α-2,6-linked disialic acid	1119:1144	α-2,6-linked disialic acid	1119:1144	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	5	36	link	α-2,6-linked	1119:1130	arg1	acid					1141:1144	α-2,6-linked disialic acid	1119:1144	α-2,6-linked disialic acid	1119:1144	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	1	37	theme	oligosaccharides	230:245	arg1	role					211:214	The functional role	196:214	The functional role of human milk oligosaccharides (HMOs)	196:252	The functional role of human milk oligosaccharides (HMOs) is closely associated with their type, composition, and structure.					
31334644	0	38	theme	Spectrometry	64:75	arg1	Analysis					77:84	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis	0:84	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.	0:194	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	5	39	theme	α-2,6-linked	1174:1185	arg1	acid					1196:1199	both α-2,3- and α-2,6-linked disialic acid	1158:1199	both α-2,3- and α-2,6-linked disialic acid	1158:1199	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	0	40	theme	P	168:168	arg1	Derivatization					180:193	Girard's Reagent P On-Target Derivatization	151:193	Girard's Reagent P On-Target Derivatization	151:193	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	3	41	theme	solid-phase	586:596	arg1	extraction					598:607	graphite carbon solid-phase extraction	570:607	graphite carbon solid-phase extraction	570:607	Here, NHMOs were separated from SHMOs by DEAE-52 anion chromatography, and lactose was removed by graphite carbon solid-phase extraction.					
31334644	4	42	theme	mass	705:708	arg1	MALDI-TOF-MS					724:735	MALDI-TOF-MS	724:735	MALDI-TOF-MS	724:735	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	42	theme	mass	705:708	arg1	spectrometry					710:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	646:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	646:736	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	43	theme	laser	662:666	arg1	MALDI-TOF-MS					724:735	MALDI-TOF-MS	724:735	MALDI-TOF-MS	724:735	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	43	theme	laser	662:666	arg1	spectrometry					710:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	646:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	646:736	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	0	44	theme	Mass	59:62	arg1	Spectrometry					64:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry	0:75	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.	0:194	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	0	45	theme	Reagent	160:166	arg1	Derivatization					180:193	Girard's Reagent P On-Target Derivatization	151:193	Girard's Reagent P On-Target Derivatization	151:193	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	4	46	theme	matrix-assisted	646:660	arg1	MALDI-TOF-MS					724:735	MALDI-TOF-MS	724:735	MALDI-TOF-MS	724:735	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	46	theme	matrix-assisted	646:660	arg1	spectrometry					710:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry	646:721	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)	646:736	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	5	47	gly	fucosylated	966:976	arg1	28					958:959	28	958:959	28	958:959	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	5	48	theme	α-2,6-linked	1082:1093	arg1	acid					1106:1109	α-2,6-linked monosialic acid	1082:1109	α-2,6-linked monosialic acid	1082:1109	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	3	49	theme	graphite	570:577	arg1	extraction					598:607	graphite carbon solid-phase extraction	570:607	graphite carbon solid-phase extraction	570:607	Here, NHMOs were separated from SHMOs by DEAE-52 anion chromatography, and lactose was removed by graphite carbon solid-phase extraction.					
31334644	2	50	theme	HMOs	353:356	arg1	difficult					361:369	difficult	361:369	difficult	361:369	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	2	50	theme	HMOs	353:356	arg1	analysis					341:348	a detailed analysis	330:348	a detailed analysis of HMOs	330:356	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	0	51	theme	On-Target	170:178	arg1	Derivatization					180:193	Girard's Reagent P On-Target Derivatization	151:193	Girard's Reagent P On-Target Derivatization	151:193	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	3	52	theme	carbon	579:584	arg1	extraction					598:607	graphite carbon solid-phase extraction	570:607	graphite carbon solid-phase extraction	570:607	Here, NHMOs were separated from SHMOs by DEAE-52 anion chromatography, and lactose was removed by graphite carbon solid-phase extraction.					
31334644	6	53	theme	HMOs	1302:1305	arg1	structure					1276:1284	structure	1276:1284	structure	1276:1284	These findings provide the groundwork for further characterization of the structure and activity of HMOs.					
31334644	6	53	theme	HMOs	1302:1305	arg1	activity					1290:1297	activity	1290:1297	activity	1290:1297	These findings provide the groundwork for further characterization of the structure and activity of HMOs.					
31334644	5	54	theme	α-2,3-linked	1046:1057	arg1	acid					1068:1071	α-2,3-linked disialic acid	1046:1071	α-2,3-linked disialic acid	1046:1071	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	4	55	theme	reagent	756:762	arg1	derivatization					776:789	reagent P on-target derivatization	756:789	Girard's reagent P on-target derivatization (GPOD)	747:796	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	55	theme	reagent	756:762	arg1	GPOD					792:795	GPOD	792:795	GPOD	792:795	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	4	56	theme	Lactose-free	610:621	arg1	NHMOs					623:627	Lactose-free NHMOs	610:627	Lactose-free NHMOs	610:627	Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard's reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD.					
31334644	5	57	link	α-2,6-linked	1174:1185	arg1	acid					1196:1199	both α-2,3- and α-2,6-linked disialic acid	1158:1199	both α-2,3- and α-2,6-linked disialic acid	1158:1199	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	5	58	theme	disialic	1059:1066	arg1	acid					1068:1071	α-2,3-linked disialic acid	1046:1071	α-2,3-linked disialic acid	1046:1071	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	2	59	theme	detailed	332:339	arg1	difficult					361:369	difficult	361:369	difficult	361:369	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	2	59	theme	detailed	332:339	arg1	analysis					341:348	a detailed analysis	330:348	a detailed analysis of HMOs	330:356	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	0	60	theme	Milk	95:98	arg1	Oligosaccharides					128:143	Human Milk Neutral and Sialylated Free Oligosaccharides	89:143	Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization	89:193	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	2	61	theme	sialylated	427:436	arg1	SHMOs					456:460	SHMOs	456:460	SHMOs	456:460	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	2	61	theme	sialylated	427:436	arg1	oligosaccharides					438:453	sialylated oligosaccharides	427:453	sialylated oligosaccharides (SHMOs) in milk	427:469	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	2	62	theme	neutral	379:385	arg1	NHMOs					405:409	NHMOs	405:409	NHMOs	405:409	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	2	62	theme	neutral	379:385	arg1	oligosaccharides					387:402	neutral oligosaccharides	379:402	neutral oligosaccharides (NHMOs)	379:410	However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk.					
31334644	5	63	link	α-2,3-linked	1046:1057	arg1	acid					1068:1071	α-2,3-linked disialic acid	1046:1071	α-2,3-linked disialic acid	1046:1071	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	0	64	theme	Human	89:93	arg1	Oligosaccharides					128:143	Human Milk Neutral and Sialylated Free Oligosaccharides	89:143	Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization	89:193	Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis of Human Milk Neutral and Sialylated Free Oligosaccharides Using Girard's Reagent P On-Target Derivatization.					
31334644	5	65	theme	α-2,3-linked	1009:1020	arg1	acid					1033:1036	α-2,3-linked monosialic acid	1009:1036	α-2,3-linked monosialic acid	1009:1036	Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid.					
31334644	3	66	theme	DEAE-52	513:519	arg1	chromatography					527:540	DEAE-52 anion chromatography	513:540	DEAE-52 anion chromatography	513:540	Here, NHMOs were separated from SHMOs by DEAE-52 anion chromatography, and lactose was removed by graphite carbon solid-phase extraction.					
30138706	3	0	theme	administered	654:665	arg1	molecules					683:691	administered ophthalmic drug molecules	654:691	administered ophthalmic drug molecules	654:691	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	3	1	theme	drug	678:681	arg1	molecules					683:691	administered ophthalmic drug molecules	654:691	administered ophthalmic drug molecules	654:691	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	8	2	theme	first-generation	1450:1465	arg1	vitreous					1467:1474	a first-generation vitreous mimic	1448:1480	a first-generation vitreous mimic	1448:1480	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	8	2	theme	first-generation	1450:1465	arg1	better					1609:1614	better	1609:1614	better	1609:1614	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	0	3	theme	porcine	53:59	arg1	models					72:77	Bovine, porcine, and ovine models	45:77	Bovine, porcine, and ovine models	45:77	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models are poor surrogates for the human vitreous.					
30138706	7	4	theme	flux	1403:1406	arg1	values					1380:1385	values	1380:1385	values of steady-state flux and diffusion coefficient	1380:1432	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	8	5	theme	hyaluronic	1505:1514	arg1	acid					1516:1519	4.5 mg/mL hyaluronic acid	1495:1519	4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa	1495:1559	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	0	6	theme	ovine	66:70	arg1	models					72:77	Bovine, porcine, and ovine models	45:77	Bovine, porcine, and ovine models	45:77	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models are poor surrogates for the human vitreous.					
30138706	5	7	theme	physical	924:931	arg1	properties					949:958	the mechanical, physical and rheological properties	908:958	the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates	908:999	In this study, the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates were investigated and compared to human vitreous.					
30138706	5	8	theme	ovine	963:967	arg1	properties					949:958	the mechanical, physical and rheological properties	908:958	the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates	908:999	In this study, the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates were investigated and compared to human vitreous.					
30138706	7	9	theme	great	1359:1363	arg1	differences					1365:1375	great differences	1359:1375	great differences in values of steady-state flux and diffusion coefficient	1359:1432	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	7	10	from	differences	1365:1375	arg1	values					1380:1385	values	1380:1385	values of steady-state flux and diffusion coefficient	1380:1432	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	7	11	theme	different	1323:1331	arg1	samples					1342:1348	the different vitreous samples	1319:1348	the different vitreous samples	1319:1348	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	3	12	theme	molecules	683:691	arg1	transport					641:649	transport	641:649	transport	641:649	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	3	12	theme	molecules	683:691	arg1	diffusion					627:635	diffusion	627:635	diffusion	627:635	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	8	13	theme	vitreous	1635:1642	arg1	samples					1663:1669	the human vitreous than the mammalian samples	1625:1669	the human vitreous than the mammalian samples investigated	1625:1682	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	8	14	theme	than	1644:1647	arg1	samples					1663:1669	the human vitreous than the mammalian samples	1625:1669	the human vitreous than the mammalian samples investigated	1625:1682	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	7	15	theme	steady-state	1390:1401	arg1	flux					1403:1406	steady-state flux	1390:1406	steady-state flux	1390:1406	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	6	16	theme	vitreous	1167:1174	arg1	samples					1176:1182	vitreous samples	1167:1182	vitreous samples	1167:1182	In addition, a bespoke Franz cell construct was used to compare the diffusion of a model drug (fluorescein) through vitreous samples.					
30138706	4	17	theme	surrogates	734:743	arg1	means					834:838	a means	832:838	a means of studying ophthalmic drug transport and diffusion	832:890	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	4	17	theme	surrogates	734:743	arg1	number					714:719	A number	712:719	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours	712:794	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	8	18	theme	human	1629:1633	arg1	samples					1663:1669	the human vitreous than the mammalian samples	1625:1669	the human vitreous than the mammalian samples investigated	1625:1682	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	1	19	theme	human	127:131	arg1	humour					142:147	The human vitreous humour	123:147	The human vitreous humour	123:147	The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age.					
30138706	1	19	theme	human	127:131	arg1	structure					166:174	a complex gel structure	152:174	a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age	152:285	The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age.					
30138706	3	20	theme	ophthalmic	667:676	arg1	molecules					683:691	administered ophthalmic drug molecules	654:691	administered ophthalmic drug molecules	654:691	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	2	21	theme	intensive	414:422	arg1	research					424:431	intensive research	414:431	intensive research	414:431	To date, the viscoelastic properties of the human vitreous gel has not been thoroughly investigated and despite many years of intensive research, an ideal vitreous substitute remains a challenge.					
30138706	4	22	theme	mammalian	724:732	arg1	bovine					753:758	bovine	753:758	bovine	753:758	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	4	22	theme	mammalian	724:732	arg1	humours					788:794	ovine vitreous humours	773:794	ovine vitreous humours	773:794	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	4	22	theme	mammalian	724:732	arg1	porcine					761:767	porcine	761:767	porcine	761:767	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	4	22	theme	mammalian	724:732	arg1	surrogates					734:743	mammalian surrogates	724:743	mammalian surrogates	724:743	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	1	23	theme	vitreous	133:140	arg1	humour					142:147	The human vitreous humour	123:147	The human vitreous humour	123:147	The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age.					
30138706	1	23	theme	vitreous	133:140	arg1	structure					166:174	a complex gel structure	152:174	a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age	152:285	The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age.					
30138706	2	24	theme	research	424:431	arg1	years					405:409	many years	400:409	many years of intensive research	400:431	To date, the viscoelastic properties of the human vitreous gel has not been thoroughly investigated and despite many years of intensive research, an ideal vitreous substitute remains a challenge.					
30138706	6	25	theme	cell	1080:1083	arg1	construct					1085:1093	a bespoke Franz cell construct	1064:1093	a bespoke Franz cell construct	1064:1093	In addition, a bespoke Franz cell construct was used to compare the diffusion of a model drug (fluorescein) through vitreous samples.					
30138706	8	26	theme	0.3 ± 0.01 Pa	1547:1559	arg1	viscosity					1534:1542	complex viscosity	1526:1542	complex viscosity of 0.3 ± 0.01 Pa	1526:1559	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	5	27	theme	bovine	983:988	arg1	surrogates					990:999	bovine surrogates	983:999	bovine surrogates	983:999	In this study, the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates were investigated and compared to human vitreous.					
30138706	7	28	theme	coefficient	1422:1432	arg1	values					1380:1385	values	1380:1385	values of steady-state flux and diffusion coefficient	1380:1432	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	6	29	theme	Franz	1074:1078	arg1	construct					1085:1093	a bespoke Franz cell construct	1064:1093	a bespoke Franz cell construct	1064:1093	In addition, a bespoke Franz cell construct was used to compare the diffusion of a model drug (fluorescein) through vitreous samples.					
30138706	7	30	theme	bovine	1242:1247	arg1	samples					1277:1283	bovine, porcine and human vitreous samples	1242:1283	bovine, porcine and human vitreous samples	1242:1283	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	0	31	theme	ex	22:23	arg1	body					38:41	the ex vivo vitreal body	18:41	the ex vivo vitreal body	18:41	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models are poor surrogates for the human vitreous.					
30138706	2	32	dep	vitreous	443:450	arg1	substitute					452:461	substitute	452:461	substitute	452:461	To date, the viscoelastic properties of the human vitreous gel has not been thoroughly investigated and despite many years of intensive research, an ideal vitreous substitute remains a challenge.					
30138706	7	33	theme	human	1262:1266	arg1	samples					1277:1283	bovine, porcine and human vitreous samples	1242:1283	bovine, porcine and human vitreous samples	1242:1283	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	3	34	theme	physical	502:509	arg1	structure					511:519	physical structure	502:519	physical structure	502:519	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	8	35	theme	mammalian	1653:1661	arg1	samples					1663:1669	the human vitreous than the mammalian samples	1625:1669	the human vitreous than the mammalian samples investigated	1625:1682	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	7	36	theme	rheological	1211:1221	arg1	properties					1223:1232	rheological properties	1211:1232	rheological properties	1211:1232	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	7	37	theme	vitreous	1333:1340	arg1	samples					1342:1348	the different vitreous samples	1319:1348	the different vitreous samples	1319:1348	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	8	38	theme	4.5 mg/mL	1495:1503	arg1	acid					1516:1519	4.5 mg/mL hyaluronic acid	1495:1519	4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa	1495:1559	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	0	39	theme	poor	83:86	arg1	Diffusion					0:8	Diffusion	0:8	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models	0:77	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models are poor surrogates for the human vitreous.					
30138706	0	39	theme	poor	83:86	arg1	surrogates					88:97	poor surrogates	83:97	poor surrogates for the human vitreous	83:120	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models are poor surrogates for the human vitreous.					
30138706	5	40	theme	surrogates	990:999	arg1	properties					949:958	the mechanical, physical and rheological properties	908:958	the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates	908:999	In this study, the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates were investigated and compared to human vitreous.					
30138706	6	41	used	used	1099:1102	arg2	construct					1085:1093	a bespoke Franz cell construct	1064:1093	a bespoke Franz cell construct	1064:1093	In addition, a bespoke Franz cell construct was used to compare the diffusion of a model drug (fluorescein) through vitreous samples.					
30138706	1	42	theme	complex	154:160	arg1	humour					142:147	The human vitreous humour	123:147	The human vitreous humour	123:147	The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age.					
30138706	1	42	theme	complex	154:160	arg1	structure					166:174	a complex gel structure	152:174	a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age	152:285	The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age.					
30138706	0	43	theme	vitreal	30:36	arg1	body					38:41	the ex vivo vitreal body	18:41	the ex vivo vitreal body	18:41	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models are poor surrogates for the human vitreous.					
30138706	6	44	theme	model	1134:1138	arg1	fluorescein					1146:1156	fluorescein	1146:1156	fluorescein	1146:1156	In addition, a bespoke Franz cell construct was used to compare the diffusion of a model drug (fluorescein) through vitreous samples.					
30138706	6	44	theme	model	1134:1138	arg1	drug					1140:1143	a model drug	1132:1143	a model drug (fluorescein)	1132:1157	In addition, a bespoke Franz cell construct was used to compare the diffusion of a model drug (fluorescein) through vitreous samples.					
30138706	3	45	theme	vitreous	543:550	arg1	structure					511:519	physical structure	502:519	physical structure	502:519	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	3	45	theme	vitreous	543:550	arg1	properties					525:534	properties	525:534	properties	525:534	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	1	46	theme	gel	162:164	arg1	humour					142:147	The human vitreous humour	123:147	The human vitreous humour	123:147	The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age.					
30138706	1	46	theme	gel	162:164	arg1	structure					166:174	a complex gel structure	152:174	a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age	152:285	The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age.					
30138706	2	47	theme	vitreous	338:345	arg1	gel					347:349	the human vitreous gel	328:349	the human vitreous gel	328:349	To date, the viscoelastic properties of the human vitreous gel has not been thoroughly investigated and despite many years of intensive research, an ideal vitreous substitute remains a challenge.					
30138706	8	48	dep	better	1609:1614	arg1	mimic					1616:1620	mimic	1616:1620	mimic	1616:1620	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	6	49	theme	drug	1140:1143	arg1	diffusion					1119:1127	the diffusion	1115:1127	the diffusion of a model drug (fluorescein) through vitreous samples	1115:1182	In addition, a bespoke Franz cell construct was used to compare the diffusion of a model drug (fluorescein) through vitreous samples.					
30138706	7	50	from	similarity	1197:1206	arg1	properties					1223:1232	rheological properties	1211:1232	rheological properties	1211:1232	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	2	51	theme	gel	347:349	arg1	properties					314:323	the viscoelastic properties	297:323	the viscoelastic properties of the human vitreous gel	297:349	To date, the viscoelastic properties of the human vitreous gel has not been thoroughly investigated and despite many years of intensive research, an ideal vitreous substitute remains a challenge.					
30138706	4	52	used	used	806:809	arg2	number					714:719	A number	712:719	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours	712:794	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	4	52	used	used	806:809	arg2	means					834:838	a means	832:838	a means of studying ophthalmic drug transport and diffusion	832:890	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	4	53	theme	vitreous	779:786	arg1	humours					788:794	ovine vitreous humours	773:794	ovine vitreous humours	773:794	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	4	53	theme	vitreous	779:786	arg1	surrogates					734:743	mammalian surrogates	724:743	mammalian surrogates	724:743	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	5	54	theme	human	1035:1039	arg1	vitreous					1041:1048	human vitreous	1035:1048	human vitreous	1035:1048	In this study, the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates were investigated and compared to human vitreous.					
30138706	2	55	theme	many	400:403	arg1	years					405:409	many years	400:409	many years of intensive research	400:431	To date, the viscoelastic properties of the human vitreous gel has not been thoroughly investigated and despite many years of intensive research, an ideal vitreous substitute remains a challenge.					
30138706	8	56	theme	complex	1526:1532	arg1	viscosity					1534:1542	complex viscosity	1526:1542	complex viscosity of 0.3 ± 0.01 Pa	1526:1559	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	0	57	theme	human	107:111	arg1	vitreous					113:120	the human vitreous	103:120	the human vitreous	103:120	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models are poor surrogates for the human vitreous.					
30138706	5	58	theme	porcine	970:976	arg1	properties					949:958	the mechanical, physical and rheological properties	908:958	the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates	908:999	In this study, the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates were investigated and compared to human vitreous.					
30138706	4	59	theme	ovine	773:777	arg1	humours					788:794	ovine vitreous humours	773:794	ovine vitreous humours	773:794	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	4	59	theme	ovine	773:777	arg1	surrogates					734:743	mammalian surrogates	724:743	mammalian surrogates	724:743	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	7	60	theme	porcine	1250:1256	arg1	samples					1277:1283	bovine, porcine and human vitreous samples	1242:1283	bovine, porcine and human vitreous samples	1242:1283	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	5	61	theme	rheological	937:947	arg1	properties					949:958	the mechanical, physical and rheological properties	908:958	the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates	908:999	In this study, the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates were investigated and compared to human vitreous.					
30138706	7	62	theme	vitreous	1268:1275	arg1	samples					1277:1283	bovine, porcine and human vitreous samples	1242:1283	bovine, porcine and human vitreous samples	1242:1283	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	0	63	dep	ex	22:23	arg1	vivo					25:28	vivo	25:28	vivo	25:28	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models are poor surrogates for the human vitreous.					
30138706	7	64	theme	fluorescein	1299:1309	arg1	diffusion					1286:1294	diffusion	1286:1294	diffusion of fluorescein through the different vitreous samples	1286:1348	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	6	65	theme	bespoke	1066:1072	arg1	construct					1085:1093	a bespoke Franz cell construct	1064:1093	a bespoke Franz cell construct	1064:1093	In addition, a bespoke Franz cell construct was used to compare the diffusion of a model drug (fluorescein) through vitreous samples.					
30138706	5	66	theme	mechanical	912:921	arg1	properties					949:958	the mechanical, physical and rheological properties	908:958	the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates	908:999	In this study, the mechanical, physical and rheological properties of ovine, porcine, and bovine surrogates were investigated and compared to human vitreous.					
30138706	3	67	theme	clear	608:612	arg1	insight					614:620	a clear insight	606:620	a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous	606:709	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	3	68	theme	therapeutic	574:584	arg1	interest					586:593	fundamental and therapeutic interest	558:593	fundamental and therapeutic interest	558:593	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	3	69	dep	structure	511:519	arg1	the					498:500	the	498:500	the	498:500	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	4	70	theme	ophthalmic	852:861	arg1	transport					868:876	ophthalmic drug transport	852:876	ophthalmic drug transport	852:876	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	2	71	theme	ideal	437:441	arg1	vitreous					443:450	an ideal vitreous	434:450	an ideal vitreous substitute	434:461	To date, the viscoelastic properties of the human vitreous gel has not been thoroughly investigated and despite many years of intensive research, an ideal vitreous substitute remains a challenge.					
30138706	4	72	theme	drug	863:866	arg1	transport					868:876	ophthalmic drug transport	852:876	ophthalmic drug transport	852:876	A number of mammalian surrogates, mainly bovine, porcine and ovine vitreous humours have been used in the literature as a means of studying ophthalmic drug transport and diffusion.					
30138706	8	73	with	acid	1516:1519	arg1	viscosity					1534:1542	complex viscosity	1526:1542	complex viscosity of 0.3 ± 0.01 Pa	1526:1559	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	2	74	theme	human	332:336	arg1	gel					347:349	the human vitreous gel	328:349	the human vitreous gel	328:349	To date, the viscoelastic properties of the human vitreous gel has not been thoroughly investigated and despite many years of intensive research, an ideal vitreous substitute remains a challenge.					
30138706	0	75	theme	Bovine	45:50	arg1	models					72:77	Bovine, porcine, and ovine models	45:77	Bovine, porcine, and ovine models	45:77	Diffusion through the ex vivo vitreal body - Bovine, porcine, and ovine models are poor surrogates for the human vitreous.					
30138706	7	76	theme	diffusion	1412:1420	arg1	coefficient					1422:1432	diffusion coefficient	1412:1432	diffusion coefficient	1412:1432	Despite the similarity in rheological properties between bovine, porcine and human vitreous samples, diffusion of fluorescein through the different vitreous samples revealed great differences in values of steady-state flux and diffusion coefficient.					
30138706	3	77	theme	fundamental	558:568	arg1	interest					586:593	fundamental and therapeutic interest	558:593	fundamental and therapeutic interest	558:593	Understanding the physical structure and properties of the vitreous is of fundamental and therapeutic interest, providing a clear insight into diffusion and transport of administered ophthalmic drug molecules into the vitreous.					
30138706	2	78	theme	viscoelastic	301:312	arg1	properties					314:323	the viscoelastic properties	297:323	the viscoelastic properties of the human vitreous gel	297:349	To date, the viscoelastic properties of the human vitreous gel has not been thoroughly investigated and despite many years of intensive research, an ideal vitreous substitute remains a challenge.					
30138706	8	79	dep	vitreous	1467:1474	arg1	mimic					1476:1480	mimic	1476:1480	mimic	1476:1480	In addition, a first-generation vitreous mimic, composed of 4.5 mg/mL hyaluronic acid with complex viscosity of 0.3 ± 0.01 Pa has been evaluated and was demonstrated to be a better mimic of the human vitreous than the mammalian samples investigated.					
30138706	1	80	theme	physical	198:205	arg1	properties					207:216	physical properties	198:216	physical properties	198:216	The human vitreous humour is a complex gel structure whose composition and physical properties can vary considerably from person to person and also change with age.					
30738167	6	0	theme	purification	1020:1031	arg1	purpose					1033:1039	water purification purpose	1014:1039	water purification purpose	1014:1039	Excellent recyclability and reusability of the adsorbent materials suggested their applicability towards textile industry and water purification purpose.					
30738167	3	1	theme	Maximum	513:519	arg1	percentage					533:542	Maximum dye removal percentage	513:542	Maximum dye removal percentage (97.37%)	513:551	Maximum dye removal percentage (97.37%) was observed with ZnS nanocomposite for the removal of biebrich scarlet.					
30738167	3	1	theme	Maximum	513:519	arg1	%					550:550	97.37%	545:550	97.37%	545:550	Maximum dye removal percentage (97.37%) was observed with ZnS nanocomposite for the removal of biebrich scarlet.					
30738167	6	2	theme	water	1014:1018	arg1	purpose					1033:1039	water purification purpose	1014:1039	water purification purpose	1014:1039	Excellent recyclability and reusability of the adsorbent materials suggested their applicability towards textile industry and water purification purpose.					
30738167	1	3	theme	RSM-CCD	174:180	arg1	scheme					269:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme	174:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	174:396	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	1	4	theme	semi-IPN	279:286	arg1	NaAla-Gel-cl-polyAAm					320:339	semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm	279:339	semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm	279:339	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	0	5	theme	scarlet	113:119	arg1	dyes					140:143	biebrich scarlet and crystal violet dyes	104:143	biebrich scarlet and crystal violet dyes	104:143	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	6	6	theme	Excellent	888:896	arg1	recyclability					898:910	Excellent recyclability	888:910	Excellent recyclability	888:910	Excellent recyclability and reusability of the adsorbent materials suggested their applicability towards textile industry and water purification purpose.					
30738167	3	7	theme	scarlet	617:623	arg1	removal					597:603	the removal	593:603	the removal of biebrich scarlet	593:623	Maximum dye removal percentage (97.37%) was observed with ZnS nanocomposite for the removal of biebrich scarlet.					
30738167	0	8	theme	biebrich	104:111	arg1	scarlet					113:119	biebrich scarlet	104:119	biebrich scarlet	104:119	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	5	9	theme	ΔS°	820:822	arg1	values					824:829	ΔG°, ΔH° and ΔS° values	807:829	ΔG°, ΔH° and ΔS° values	807:829	ΔG°, ΔH° and ΔS° values indicated the thermodynamic feasibility of the reaction.					
30738167	1	10	theme	semi	289:292	arg1	network					311:317	semi interpenetrating network	289:317	semi interpenetrating network	289:317	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	0	11	theme	crystal	125:131	arg1	violet					133:138	crystal violet	125:138	crystal violet	125:138	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	6	12	theme	textile	993:999	arg1	industry					1001:1008	textile industry	993:1008	textile industry	993:1008	Excellent recyclability and reusability of the adsorbent materials suggested their applicability towards textile industry and water purification purpose.					
30738167	2	13	theme	%	510:510	arg1	percentage					489:498	maximum swelling percentage	472:498	maximum swelling percentage of 3191.73%	472:510	Under optimized reaction parameters semi-IPN NaAla-Gel-cl-polyAAm showed maximum swelling percentage of 3191.73%.					
30738167	2	14	theme	maximum	472:478	arg1	percentage					489:498	maximum swelling percentage	472:498	maximum swelling percentage of 3191.73%	472:510	Under optimized reaction parameters semi-IPN NaAla-Gel-cl-polyAAm showed maximum swelling percentage of 3191.73%.					
30738167	3	15	theme	removal	525:531	arg1	percentage					533:542	Maximum dye removal percentage	513:542	Maximum dye removal percentage (97.37%)	513:551	Maximum dye removal percentage (97.37%) was observed with ZnS nanocomposite for the removal of biebrich scarlet.					
30738167	3	15	theme	removal	525:531	arg1	%					550:550	97.37%	545:550	97.37%	545:550	Maximum dye removal percentage (97.37%) was observed with ZnS nanocomposite for the removal of biebrich scarlet.					
30738167	4	16	theme	Langmuir	670:677	arg1	adsorption					694:703	Langmuir and Freundlich adsorption	670:703	adsorption	694:703	The adsorption isotherm data indicated that Langmuir and Freundlich adsorption isotherm fitted well for biebrich scarlet (R2 = 0.964) and crystal violet (R2 = 0.960), respectively.					
30738167	5	17	theme	thermodynamic	845:857	arg1	feasibility					859:869	the thermodynamic feasibility	841:869	the thermodynamic feasibility of the reaction	841:885	ΔG°, ΔH° and ΔS° values indicated the thermodynamic feasibility of the reaction.					
30738167	3	18	theme	biebrich	608:615	arg1	scarlet					617:623	biebrich scarlet	608:623	biebrich scarlet	608:623	Maximum dye removal percentage (97.37%) was observed with ZnS nanocomposite for the removal of biebrich scarlet.					
30738167	1	19	theme	Response	183:190	arg1	Methodology					200:210	Response Surface Methodology	183:210	Response Surface Methodology integrated Central composite Design	183:246	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	5	20	theme	reaction	878:885	arg1	feasibility					859:869	the thermodynamic feasibility	841:869	the thermodynamic feasibility of the reaction	841:885	ΔG°, ΔH° and ΔS° values indicated the thermodynamic feasibility of the reaction.					
30738167	4	21	theme	isotherm	641:648	arg1	data					650:653	The adsorption isotherm data	626:653	The adsorption isotherm data	626:653	The adsorption isotherm data indicated that Langmuir and Freundlich adsorption isotherm fitted well for biebrich scarlet (R2 = 0.964) and crystal violet (R2 = 0.960), respectively.					
30738167	4	22	theme	adsorption	630:639	arg1	data					650:653	The adsorption isotherm data	626:653	The adsorption isotherm data	626:653	The adsorption isotherm data indicated that Langmuir and Freundlich adsorption isotherm fitted well for biebrich scarlet (R2 = 0.964) and crystal violet (R2 = 0.960), respectively.					
30738167	1	23	theme	Surface	192:198	arg1	Methodology					200:210	Response Surface Methodology	183:210	Response Surface Methodology integrated Central composite Design	183:246	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	1	24	theme	interpenetrating	294:309	arg1	network					311:317	semi interpenetrating network	289:317	semi interpenetrating network	289:317	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	1	25	dep	RSM-CCD	174:180	arg1	Methodology					200:210	Response Surface Methodology	183:210	Response Surface Methodology integrated Central composite Design	183:246	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	5	26	theme	ΔG°	807:809	arg1	values					824:829	ΔG°, ΔH° and ΔS° values	807:829	ΔG°, ΔH° and ΔS° values	807:829	ΔG°, ΔH° and ΔS° values indicated the thermodynamic feasibility of the reaction.					
30738167	3	27	theme	dye	521:523	arg1	percentage					533:542	Maximum dye removal percentage	513:542	Maximum dye removal percentage (97.37%)	513:551	Maximum dye removal percentage (97.37%) was observed with ZnS nanocomposite for the removal of biebrich scarlet.					
30738167	3	27	theme	dye	521:523	arg1	%					550:550	97.37%	545:550	97.37%	545:550	Maximum dye removal percentage (97.37%) was observed with ZnS nanocomposite for the removal of biebrich scarlet.					
30738167	0	28	theme	alginate/gelatin	25:40	arg1	hydrogel					66:73	sodium alginate/gelatin based ZnS-nanocomposite hydrogel	18:73	sodium alginate/gelatin based ZnS-nanocomposite hydrogel	18:73	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	0	29	theme	dyes	140:143	arg1	removal					93:99	the effective removal	79:99	the effective removal of biebrich scarlet and crystal violet dyes	79:143	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	1	30	theme	integrated	212:221	arg1	Design					241:246	integrated Central composite Design	212:246	Response Surface Methodology integrated Central composite Design	183:246	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	0	31	theme	sodium	18:23	arg1	hydrogel					66:73	sodium alginate/gelatin based ZnS-nanocomposite hydrogel	18:73	sodium alginate/gelatin based ZnS-nanocomposite hydrogel	18:73	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	0	32	theme	violet	133:138	arg1	dyes					140:143	biebrich scarlet and crystal violet dyes	104:143	biebrich scarlet and crystal violet dyes	104:143	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	2	33	theme	reaction	415:422	arg1	parameters					424:433	optimized reaction parameters	405:433	optimized reaction parameters	405:433	Under optimized reaction parameters semi-IPN NaAla-Gel-cl-polyAAm showed maximum swelling percentage of 3191.73%.					
30738167	1	34	theme	Central	223:229	arg1	Design					241:246	integrated Central composite Design	212:246	Response Surface Methodology integrated Central composite Design	183:246	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	0	35	theme	ZnS-nanocomposite	48:64	arg1	hydrogel					66:73	sodium alginate/gelatin based ZnS-nanocomposite hydrogel	18:73	sodium alginate/gelatin based ZnS-nanocomposite hydrogel	18:73	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	1	36	theme	NaAla-Gel-cl-polyAAm	320:339	arg1	scheme					269:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme	174:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	174:396	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	1	37	theme	composite	231:239	arg1	Design					241:246	integrated Central composite Design	212:246	Response Surface Methodology integrated Central composite Design	183:246	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	0	38	theme	based	42:46	arg1	hydrogel					66:73	sodium alginate/gelatin based ZnS-nanocomposite hydrogel	18:73	sodium alginate/gelatin based ZnS-nanocomposite hydrogel	18:73	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	6	39	theme	materials	945:953	arg1	reusability					916:926	reusability	916:926	reusability	916:926	Excellent recyclability and reusability of the adsorbent materials suggested their applicability towards textile industry and water purification purpose.					
30738167	6	39	theme	materials	945:953	arg1	recyclability					898:910	Excellent recyclability	888:910	Excellent recyclability	888:910	Excellent recyclability and reusability of the adsorbent materials suggested their applicability towards textile industry and water purification purpose.					
30738167	1	40	dep	Methodology	200:210	arg1	Design					241:246	integrated Central composite Design	212:246	Response Surface Methodology integrated Central composite Design	183:246	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	4	41	theme	biebrich	730:737	arg1	R2 = 0.964					748:757	R2 = 0.964	748:757	R2 = 0.964	748:757	The adsorption isotherm data indicated that Langmuir and Freundlich adsorption isotherm fitted well for biebrich scarlet (R2 = 0.964) and crystal violet (R2 = 0.960), respectively.					
30738167	4	41	theme	biebrich	730:737	arg1	scarlet					739:745	biebrich scarlet	730:745	biebrich scarlet (R2 = 0.964)	730:758	The adsorption isotherm data indicated that Langmuir and Freundlich adsorption isotherm fitted well for biebrich scarlet (R2 = 0.964) and crystal violet (R2 = 0.960), respectively.					
30738167	1	42	theme	ZnS	345:347	arg1	NaAla-Gel-cl-polyAAm/ZnS					373:396	ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	345:396	ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	345:396	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	6	43	theme	adsorbent	935:943	arg1	materials					945:953	the adsorbent materials	931:953	the adsorbent materials	931:953	Excellent recyclability and reusability of the adsorbent materials suggested their applicability towards textile industry and water purification purpose.					
30738167	2	44	theme	optimized	405:413	arg1	parameters					424:433	optimized reaction parameters	405:433	optimized reaction parameters	405:433	Under optimized reaction parameters semi-IPN NaAla-Gel-cl-polyAAm showed maximum swelling percentage of 3191.73%.					
30738167	4	45	theme	Freundlich	683:692	arg1	adsorption					694:703	Langmuir and Freundlich adsorption	670:703	adsorption	694:703	The adsorption isotherm data indicated that Langmuir and Freundlich adsorption isotherm fitted well for biebrich scarlet (R2 = 0.964) and crystal violet (R2 = 0.960), respectively.					
30738167	1	46	theme	nanocomposite	349:361	arg1	NaAla-Gel-cl-polyAAm/ZnS					373:396	ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	345:396	ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	345:396	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	3	47	theme	ZnS	571:573	arg1	nanocomposite					575:587	ZnS nanocomposite	571:587	ZnS nanocomposite	571:587	Maximum dye removal percentage (97.37%) was observed with ZnS nanocomposite for the removal of biebrich scarlet.					
30738167	1	48	theme	adsorbent	363:371	arg1	NaAla-Gel-cl-polyAAm/ZnS					373:396	ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	345:396	ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	345:396	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	4	49	theme	adsorption	694:703	arg1	isotherm					705:712	Langmuir and Freundlich adsorption isotherm	670:712	Langmuir and Freundlich adsorption isotherm	670:712	The adsorption isotherm data indicated that Langmuir and Freundlich adsorption isotherm fitted well for biebrich scarlet (R2 = 0.964) and crystal violet (R2 = 0.960), respectively.					
30738167	4	50	theme	crystal	764:770	arg1	R2 = 0.960					780:789	R2 = 0.960	780:789	R2 = 0.960	780:789	The adsorption isotherm data indicated that Langmuir and Freundlich adsorption isotherm fitted well for biebrich scarlet (R2 = 0.964) and crystal violet (R2 = 0.960), respectively.					
30738167	4	50	theme	crystal	764:770	arg1	violet					772:777	crystal violet	764:777	crystal violet (R2 = 0.960)	764:790	The adsorption isotherm data indicated that Langmuir and Freundlich adsorption isotherm fitted well for biebrich scarlet (R2 = 0.964) and crystal violet (R2 = 0.960), respectively.					
30738167	1	51	theme	optimized	249:257	arg1	scheme					269:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme	174:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	174:396	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	0	52	theme	effective	83:91	arg1	removal					93:99	the effective removal	79:99	the effective removal of biebrich scarlet and crystal violet dyes	79:143	RSM-CCD optimized sodium alginate/gelatin based ZnS-nanocomposite hydrogel for the effective removal of biebrich scarlet and crystal violet dyes.					
30738167	1	53	dep	semi-IPN	279:286	arg1	network					311:317	semi interpenetrating network	289:317	semi interpenetrating network	289:317	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	1	54	theme	present	150:156	arg1	work					158:161	The present work	146:161	The present work	146:161	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	2	55	theme	swelling	480:487	arg1	percentage					489:498	maximum swelling percentage	472:498	maximum swelling percentage of 3191.73%	472:510	Under optimized reaction parameters semi-IPN NaAla-Gel-cl-polyAAm showed maximum swelling percentage of 3191.73%.					
30738167	1	56	theme	synthesis	259:267	arg1	scheme					269:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme	174:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	174:396	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	1	57	theme	NaAla-Gel-cl-polyAAm/ZnS	373:396	arg1	scheme					269:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme	174:274	RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS	174:396	The present work represents RSM-CCD (Response Surface Methodology integrated Central composite Design) optimized synthesis scheme of semi-IPN (semi interpenetrating network) NaAla-Gel-cl-polyAAm and ZnS nanocomposite adsorbent NaAla-Gel-cl-polyAAm/ZnS.					
30738167	2	58	theme	semi-IPN	435:442	arg1	NaAla-Gel-cl-polyAAm					444:463	semi-IPN NaAla-Gel-cl-polyAAm	435:463	semi-IPN NaAla-Gel-cl-polyAAm	435:463	Under optimized reaction parameters semi-IPN NaAla-Gel-cl-polyAAm showed maximum swelling percentage of 3191.73%.					
30738167	5	59	theme	ΔH°	812:814	arg1	values					824:829	ΔG°, ΔH° and ΔS° values	807:829	ΔG°, ΔH° and ΔS° values	807:829	ΔG°, ΔH° and ΔS° values indicated the thermodynamic feasibility of the reaction.					
29542825	10	0	theme	gut	1422:1424	arg1	infections					1404:1413	infections	1404:1413	infections of the gut in carp	1404:1432	This might be protective against pathogenic bacteria and, therefore, feeding with β-glucan may provide protection against infections of the gut in carp.					
29542825	0	1	dep	Aeromonas	97:105	arg1	hydrophila					107:116	hydrophila	107:116	hydrophila	107:116	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	1	2	theme	Aeromonas	307:315	arg1	hydrophila					317:326	Aeromonas hydrophila	307:326	Aeromonas hydrophila	307:326	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	3	3	from	group	490:494	arg1	half					463:466	half	463:466	half of the carp from each group	463:494	Fourteen days after feeding, half of the carp from each group were intubated with 109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila.					
29542825	10	4	from	gut	1422:1424	arg1	carp					1429:1432	carp	1429:1432	carp	1429:1432	This might be protective against pathogenic bacteria and, therefore, feeding with β-glucan may provide protection against infections of the gut in carp.					
29542825	1	5	theme	hydrophila	317:326	arg1	application					292:302	oral application	287:302	oral application of Aeromonas hydrophila	287:326	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	10	6	theme	pathogenic	1315:1324	arg1	bacteria					1326:1333	pathogenic bacteria	1315:1333	pathogenic bacteria	1315:1333	This might be protective against pathogenic bacteria and, therefore, feeding with β-glucan may provide protection against infections of the gut in carp.					
29542825	3	7	dep	days	443:446	arg1	feeding					454:460	feeding	454:460	feeding	454:460	Fourteen days after feeding, half of the carp from each group were intubated with 109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila.					
29542825	5	8	theme	control	874:880	arg1	group					882:886	the control group	870:886	the control group	870:886	The reaction of the mucosa and the microbiota to an A. hydrophila intubation differed in carp fed with β-glucan compared to carp from the control group.					
29542825	1	9	theme	common	238:243	arg1	carpio					260:265	Cyprinus carpio	251:265	Cyprinus carpio	251:265	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	1	9	theme	common	238:243	arg1	carp					245:248	common carp	238:248	common carp (Cyprinus carpio)	238:266	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	6	10	theme	fed	901:903	arg1	carp					905:908	β-glucan fed carp	892:908	β-glucan fed carp	892:908	In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher.					
29542825	1	11	theme	carp	245:248	arg1	gut					231:233	the gut	227:233	the gut of common carp (Cyprinus carpio)	227:266	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	7	12	from	groups	1061:1066	arg1	different					1022:1030	different	1022:1030	different	1022:1030	Bacterial composition was different for carp from both treatment groups.					
29542825	9	13	theme	bacteria	1272:1279	arg1	numbers					1261:1267	high numbers	1256:1267	high numbers of bacteria	1256:1279	Mucus was obviously released from the goblet cells and was probably washed out of the gut together with high numbers of bacteria.					
29542825	7	14	theme	Bacterial	996:1004	arg1	composition					1006:1016	Bacterial composition	996:1016	Bacterial composition	996:1016	Bacterial composition was different for carp from both treatment groups.					
29542825	8	15	theme	goblet	1096:1101	arg1	cells					1103:1107	mucin filled goblet cells	1083:1107	mucin filled goblet cells	1083:1107	The number of mucin filled goblet cells was reduced in carp fed the β-glucan diet.					
29542825	2	16	theme	1	373:373	arg1	%					374:374	%	374:374	%	374:374	Carp received either feed supplemented with 1% MacroGard® , a β-1,3/1,6-glucan, or a β-glucan-free diet.					
29542825	0	17	theme	bacterial	74:82	arg1	challenge					84:92	a bacterial challenge	72:92	a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan	72:163	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	9	18	theme	goblet	1190:1195	arg1	cells					1197:1201	the goblet cells	1186:1201	the goblet cells	1186:1201	Mucus was obviously released from the goblet cells and was probably washed out of the gut together with high numbers of bacteria.					
29542825	7	19	theme	treatment	1051:1059	arg1	groups					1061:1066	both treatment groups	1046:1066	both treatment groups	1046:1066	Bacterial composition was different for carp from both treatment groups.					
29542825	4	20	dep	12 hr	611:615	arg1	application					633:643	application	633:643	application	633:643	Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE).					
29542825	4	21	theme	Gut	588:590	arg1	samples					592:598	Gut samples	588:598	Gut samples	588:598	Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE).					
29542825	8	22	theme	filled	1089:1094	arg1	cells					1103:1107	mucin filled goblet cells	1083:1107	mucin filled goblet cells	1083:1107	The number of mucin filled goblet cells was reduced in carp fed the β-glucan diet.					
29542825	6	23	theme	bacterial	966:974	arg1	species					976:982	bacterial species	966:982	bacterial species	966:982	In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher.					
29542825	8	24	theme	mucin	1083:1087	arg1	cells					1103:1107	mucin filled goblet cells	1083:1107	mucin filled goblet cells	1083:1107	The number of mucin filled goblet cells was reduced in carp fed the β-glucan diet.					
29542825	6	25	theme	β-glucan	892:899	arg1	carp					905:908	β-glucan fed carp	892:908	β-glucan fed carp	892:908	In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher.					
29542825	3	26	theme	colony-forming	520:533	arg1	CFU					542:544	CFU	542:544	CFU	542:544	Fourteen days after feeding, half of the carp from each group were intubated with 109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila.					
29542825	3	26	theme	colony-forming	520:533	arg1	units					535:539	109 colony-forming units	516:539	109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila	516:585	Fourteen days after feeding, half of the carp from each group were intubated with 109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila.					
29542825	8	27	theme	β-glucan	1137:1144	arg1	diet					1146:1149	the β-glucan diet	1133:1149	the β-glucan diet	1133:1149	The number of mucin filled goblet cells was reduced in carp fed the β-glucan diet.					
29542825	0	28	theme	intestinal	16:25	arg1	barrier					35:41	the intestinal mucosal barrier	12:41	the intestinal mucosal barrier of carp (Cyprinus carpio)	12:67	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	1	29	theme	Cyprinus	251:258	arg1	carpio					260:265	Cyprinus carpio	251:265	Cyprinus carpio	251:265	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	1	29	theme	Cyprinus	251:258	arg1	carp					245:248	common carp	238:248	common carp (Cyprinus carpio)	238:266	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	0	30	theme	Aeromonas	97:105	arg1	intubation					118:127	Aeromonas hydrophila intubation	97:127	Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan	97:163	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	4	31	theme	biological	694:703	arg1	RT-PCR-DGGE					722:732	RT-PCR-DGGE	722:732	RT-PCR-DGGE	722:732	Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE).					
29542825	4	31	theme	biological	694:703	arg1	techniques					705:714	microbiological and molecular biological techniques	664:714	techniques	705:714	Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE).					
29542825	6	32	theme	species	976:982	arg1	higher					988:993	higher	988:993	higher	988:993	In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher.					
29542825	6	32	theme	species	976:982	arg1	number					956:961	the number	952:961	the number of bacterial species	952:982	In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher.					
29542825	1	33	theme	dietary	180:186	arg1	β-glucan					188:195	dietary β-glucan	180:195	dietary β-glucan	180:195	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	4	34	theme	microbiological	664:678	arg1	RT-PCR-DGGE					722:732	RT-PCR-DGGE	722:732	RT-PCR-DGGE	722:732	Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE).					
29542825	4	34	theme	microbiological	664:678	arg1	techniques					705:714	microbiological and molecular biological techniques	664:714	techniques	705:714	Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE).					
29542825	3	35	theme	hydrophila	576:585	arg1	strain					563:568	a pathogenic strain	550:568	a pathogenic strain of A. hydrophila	550:585	Fourteen days after feeding, half of the carp from each group were intubated with 109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila.					
29542825	4	36	theme	molecular	684:692	arg1	RT-PCR-DGGE					722:732	RT-PCR-DGGE	722:732	RT-PCR-DGGE	722:732	Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE).					
29542825	4	36	theme	molecular	684:692	arg1	techniques					705:714	microbiological and molecular biological techniques	664:714	techniques	705:714	Gut samples were taken 12 hr to 7 days after application and analysed using microbiological and molecular biological techniques (NGS, RT-PCR-DGGE).					
29542825	10	37	with	feeding	1351:1357	arg1	β-glucan					1364:1371	β-glucan	1364:1371	β-glucan	1364:1371	This might be protective against pathogenic bacteria and, therefore, feeding with β-glucan may provide protection against infections of the gut in carp.					
29542825	1	38	theme	β-glucan	188:195	arg1	effect					170:175	The effect	166:175	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio)	166:266	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	0	39	theme	barrier	35:41	arg1	Response					0:7	Response	0:7	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.	0:164	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	9	40	attach	released	1172:1179	arg2	Mucus					1152:1156	Mucus	1152:1156	Mucus	1152:1156	Mucus was obviously released from the goblet cells and was probably washed out of the gut together with high numbers of bacteria.					
29542825	9	40	attach	released	1172:1179	arg1	cells					1197:1201	the goblet cells	1186:1201	the goblet cells	1186:1201	Mucus was obviously released from the goblet cells and was probably washed out of the gut together with high numbers of bacteria.					
29542825	0	41	theme	mucosal	27:33	arg1	barrier					35:41	the intestinal mucosal barrier	12:41	the intestinal mucosal barrier of carp (Cyprinus carpio)	12:67	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	9	42	theme	high	1256:1259	arg1	numbers					1261:1267	high numbers	1256:1267	high numbers of bacteria	1256:1279	Mucus was obviously released from the goblet cells and was probably washed out of the gut together with high numbers of bacteria.					
29542825	10	43	from	carp	1429:1432	arg1	infections					1404:1413	infections	1404:1413	infections of the gut in carp	1404:1432	This might be protective against pathogenic bacteria and, therefore, feeding with β-glucan may provide protection against infections of the gut in carp.					
29542825	8	44	theme	cells	1103:1107	arg1	number					1073:1078	The number	1069:1078	The number of mucin filled goblet cells	1069:1107	The number of mucin filled goblet cells was reduced in carp fed the β-glucan diet.					
29542825	2	45	theme	%	374:374	arg1	MacroGard®					376:385	1% MacroGard®	373:385	1% MacroGard®	373:385	Carp received either feed supplemented with 1% MacroGard® , a β-1,3/1,6-glucan, or a β-glucan-free diet.					
29542825	0	46	theme	carp	46:49	arg1	barrier					35:41	the intestinal mucosal barrier	12:41	the intestinal mucosal barrier of carp (Cyprinus carpio)	12:67	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	2	47	theme	β-glucan-free	414:426	arg1	diet					428:431	a β-glucan-free diet	412:431	a β-glucan-free diet	412:431	Carp received either feed supplemented with 1% MacroGard® , a β-1,3/1,6-glucan, or a β-glucan-free diet.					
29542825	10	48	from	infections	1404:1413	arg1	carp					1429:1432	carp	1429:1432	carp	1429:1432	This might be protective against pathogenic bacteria and, therefore, feeding with β-glucan may provide protection against infections of the gut in carp.					
29542825	5	49	dep	A.	788:789	arg1	hydrophila					791:800	hydrophila	791:800	hydrophila	791:800	The reaction of the mucosa and the microbiota to an A. hydrophila intubation differed in carp fed with β-glucan compared to carp from the control group.					
29542825	0	50	with	intubation	118:127	arg1	β-1,3/1,6-glucan					148:163	β-1,3/1,6-glucan	148:163	β-1,3/1,6-glucan	148:163	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	1	51	theme	oral	287:290	arg1	application					292:302	oral application	287:302	oral application of Aeromonas hydrophila	287:326	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	3	52	theme	pathogenic	552:561	arg1	strain					563:568	a pathogenic strain	550:568	a pathogenic strain of A. hydrophila	550:585	Fourteen days after feeding, half of the carp from each group were intubated with 109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila.					
29542825	1	53	theme	bacterial	204:212	arg1	community					214:222	the bacterial community	200:222	the bacterial community in the gut of common carp (Cyprinus carpio)	200:266	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	1	54	from	community	214:222	arg1	gut					231:233	the gut	227:233	the gut of common carp (Cyprinus carpio)	227:266	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	3	55	theme	strain	563:568	arg1	CFU					542:544	CFU	542:544	CFU	542:544	Fourteen days after feeding, half of the carp from each group were intubated with 109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila.					
29542825	3	55	theme	strain	563:568	arg1	units					535:539	109 colony-forming units	516:539	109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila	516:585	Fourteen days after feeding, half of the carp from each group were intubated with 109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila.					
29542825	1	56	from	effect	170:175	arg1	community					214:222	the bacterial community	200:222	the bacterial community in the gut of common carp (Cyprinus carpio)	200:266	The effect of dietary β-glucan on the bacterial community in the gut of common carp (Cyprinus carpio) was examined after oral application of Aeromonas hydrophila.					
29542825	0	57	theme	Cyprinus	52:59	arg1	carpio					61:66	Cyprinus carpio	52:66	Cyprinus carpio	52:66	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	0	57	theme	Cyprinus	52:59	arg1	carp					46:49	carp	46:49	carp (Cyprinus carpio)	46:67	Response of the intestinal mucosal barrier of carp (Cyprinus carpio) to a bacterial challenge by Aeromonas hydrophila intubation after feeding with β-1,3/1,6-glucan.					
29542825	5	58	theme	A.	788:789	arg1	intubation					802:811	an A. hydrophila intubation	785:811	an A. hydrophila intubation	785:811	The reaction of the mucosa and the microbiota to an A. hydrophila intubation differed in carp fed with β-glucan compared to carp from the control group.					
29542825	6	59	theme	bacterial	921:929	arg1	lower					942:946	lower	942:946	lower	942:946	In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher.					
29542825	6	59	theme	bacterial	921:929	arg1	amount					931:936	the total bacterial amount	911:936	the total bacterial amount	911:936	In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher.					
29542825	5	60	theme	mucosa	756:761	arg1	microbiota					771:780	the microbiota	767:780	the microbiota to an A. hydrophila intubation	767:811	The reaction of the mucosa and the microbiota to an A. hydrophila intubation differed in carp fed with β-glucan compared to carp from the control group.					
29542825	5	60	theme	mucosa	756:761	arg1	reaction					740:747	The reaction	736:747	The reaction of the mucosa	736:761	The reaction of the mucosa and the microbiota to an A. hydrophila intubation differed in carp fed with β-glucan compared to carp from the control group.					
29542825	3	61	theme	carp	475:478	arg1	half					463:466	half	463:466	half of the carp from each group	463:494	Fourteen days after feeding, half of the carp from each group were intubated with 109 colony-forming units (CFU) of a pathogenic strain of A. hydrophila.					
29542825	6	62	theme	total	915:919	arg1	lower					942:946	lower	942:946	lower	942:946	In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher.					
29542825	6	62	theme	total	915:919	arg1	amount					931:936	the total bacterial amount	911:936	the total bacterial amount	911:936	In β-glucan fed carp, the total bacterial amount was lower but the number of bacterial species was higher.					
29790251	0	0	theme	bone	111:114	arg1	regeneration					116:127	bone regeneration	111:127	bone regeneration	111:127	Chitosan/biphasic calcium phosphate scaffolds functionalized with BMP-2-encapsulated nanoparticles and RGD for bone regeneration.					
29790251	6	1	theme	desolvation	946:956	arg1	method					958:963	a desolvation method	944:963	a desolvation method	944:963	The BMP-2-encapsulated BSA nanoparticles were prepared via a desolvation method and then coated with CS and oxidized alginate to achieve sustained release of BMP-2.					
29790251	1	2	theme	porous	287:292	arg1	structures					294:303	highly porous structures	280:303	highly porous structures	280:303	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	7	3	theme	integrin	1200:1207	arg1	surface					1217:1223	integrin binding surface	1200:1223	integrin binding surface	1200:1223	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	1	4	theme	tissue	197:202	arg1	scaffolds					204:212	tissue scaffolds	197:212	tissue scaffolds	197:212	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	1	5	theme	tissue	389:394	arg1	regeneration					396:407	tissue regeneration	389:407	tissue regeneration	389:407	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	7	6	theme	In	1050:1051	arg1	culture					1064:1070	In vitro cell culture	1050:1070	In vitro cell culture	1050:1070	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	9	7	dep	©	1506:1506	arg1	Inc.					1532:1535	Inc.	1532:1535	Inc.	1532:1535	© 2018 Wiley Periodicals, Inc.					
29790251	1	8	contain	carry	327:331	arg1	which					215:219	which	215:219	which	215:219	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	1	8	contain	carry	327:331	arg2	molecules					343:351	signaling molecules	333:351	signaling molecules that can mediate bone formation and tissue regeneration	333:407	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	1	9	dep	require	170:176	arg1	carry					327:331	carry	327:331	effectively carry signaling molecules that can mediate bone formation and tissue regeneration	315:407	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	1	9	dep	require	170:176	arg1	exhibit					237:243	exhibit	237:243	exhibit suitable mechanical properties and highly porous structures	237:303	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	4	10	theme	freeze-drying	774:786	arg1	method					788:793	the freeze-drying method	770:793	the freeze-drying method	770:793	First, we synthesized CS/BCP composite bionic scaffolds via the freeze-drying method.					
29790251	4	11	theme	bionic	749:754	arg1	scaffolds					756:764	CS/BCP composite bionic scaffolds	732:764	CS/BCP composite bionic scaffolds	732:764	First, we synthesized CS/BCP composite bionic scaffolds via the freeze-drying method.					
29790251	8	12	theme	favorable	1385:1393	arg1	microenvironment					1395:1410	a favorable microenvironment	1383:1410	a favorable microenvironment for bone formation	1383:1429	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	8	13	theme	tissue	1486:1491	arg1	engineering					1493:1503	bone tissue engineering	1481:1503	bone tissue engineering	1481:1503	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	1	14	theme	scaffolds	204:212	arg1	improvement					182:192	the improvement	178:192	the improvement of tissue scaffolds	178:212	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	5	15	theme	RGD	802:804	arg1	peptides					806:813	RGD peptides	802:813	RGD peptides	802:813	Then, RGD peptides were covalently conjugated onto the scaffolds via the EDC/NHS method.					
29790251	5	16	theme	EDC/NHS	869:875	arg1	method					877:882	the EDC/NHS method	865:882	the EDC/NHS method	865:882	Then, RGD peptides were covalently conjugated onto the scaffolds via the EDC/NHS method.					
29790251	7	17	dep	in	1076:1077	arg1	vivo					1079:1082	vivo	1079:1082	vivo	1079:1082	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	0	18	theme	calcium	18:24	arg1	phosphate					26:34	Chitosan/biphasic calcium phosphate	0:34	Chitosan/biphasic calcium phosphate	0:34	Chitosan/biphasic calcium phosphate scaffolds functionalized with BMP-2-encapsulated nanoparticles and RGD for bone regeneration.					
29790251	6	19	theme	sustained	1022:1030	arg1	release					1032:1038	sustained release	1022:1038	sustained release of BMP-2	1022:1047	The BMP-2-encapsulated BSA nanoparticles were prepared via a desolvation method and then coated with CS and oxidized alginate to achieve sustained release of BMP-2.					
29790251	10	20	dep	106A	1564:1567	arg1	2613-2624					1570:1578	2613-2624	1570:1578	2613-2624	1570:1578	J Biomed Mater Res Part A: 106A: 2613-2624, 2018.					
29790251	10	21	theme	Part	1556:1559	arg1	A					1561:1561	Part A	1556:1561	J Biomed Mater Res Part A: 106A: 2613-2624, 2018.	1537:1585	J Biomed Mater Res Part A: 106A: 2613-2624, 2018.					
29790251	3	22	theme	bone	627:630	arg1	ECM					654:656	ECM	654:656	ECM	654:656	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	3	22	theme	bone	627:630	arg1	matrix					646:651	natural bone extracellular matrix	619:651	natural bone extracellular matrix (ECM) in terms of composition and structural properties	619:707	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	0	23	theme	Chitosan/biphasic	0:16	arg1	phosphate					26:34	Chitosan/biphasic calcium phosphate	0:34	Chitosan/biphasic calcium phosphate	0:34	Chitosan/biphasic calcium phosphate scaffolds functionalized with BMP-2-encapsulated nanoparticles and RGD for bone regeneration.					
29790251	6	24	theme	BMP-2	1043:1047	arg1	release					1032:1038	sustained release	1022:1038	sustained release of BMP-2	1022:1047	The BMP-2-encapsulated BSA nanoparticles were prepared via a desolvation method and then coated with CS and oxidized alginate to achieve sustained release of BMP-2.					
29790251	7	25	theme	cell	1157:1160	arg1	attachment					1162:1171	cell attachment	1157:1171	cell attachment	1157:1171	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	3	26	theme	extracellular	632:644	arg1	ECM					654:656	ECM	654:656	ECM	654:656	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	3	26	theme	extracellular	632:644	arg1	matrix					646:651	natural bone extracellular matrix	619:651	natural bone extracellular matrix (ECM) in terms of composition and structural properties	619:707	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	3	27	from	matrix	646:651	arg1	terms					662:666	terms	662:666	terms of composition and structural properties	662:707	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	2	28	theme	present	417:423	arg1	study					425:429	the present study	413:429	the present study	413:429	In the present study, we established chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles.					
29790251	7	29	theme	osteogenic	1242:1251	arg1	differentiation					1253:1267	osteogenic differentiation	1242:1267	osteogenic differentiation	1242:1267	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	7	30	dep	In	1050:1051	arg1	vitro					1053:1057	vitro	1053:1057	vitro	1053:1057	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	2	31	theme	phosphate	473:481	arg1	scaffolds					492:500	chitosan/biphasic calcium phosphate (CS/BCP) scaffolds	447:500	chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles	447:569	In the present study, we established chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles.					
29790251	3	32	theme	properties	698:707	arg1	terms					662:666	terms	662:666	terms of composition and structural properties	662:707	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	3	33	theme	natural	619:625	arg1	ECM					654:656	ECM	654:656	ECM	654:656	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	3	33	theme	natural	619:625	arg1	matrix					646:651	natural bone extracellular matrix	619:651	natural bone extracellular matrix (ECM) in terms of composition and structural properties	619:707	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	2	34	theme	calcium	465:471	arg1	CS/BCP					484:489	CS/BCP	484:489	CS/BCP	484:489	In the present study, we established chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles.					
29790251	2	34	theme	calcium	465:471	arg1	phosphate					473:481	chitosan/biphasic calcium phosphate	447:481	chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles	447:569	In the present study, we established chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles.					
29790251	1	35	theme	signaling	333:341	arg1	molecules					343:351	signaling molecules	333:351	signaling molecules that can mediate bone formation and tissue regeneration	333:407	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	8	36	theme	bone	1416:1419	arg1	formation					1421:1429	bone formation	1416:1429	bone formation	1416:1429	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	8	37	theme	potential	1449:1457	arg1	candidates					1459:1468	potential candidates	1449:1468	potential candidates for use in bone tissue engineering	1449:1503	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	8	37	theme	potential	1449:1457	arg1	scaffolds					1308:1316	the bioceramic/biopolymer scaffolds	1282:1316	the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules	1282:1359	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	2	38	theme	chitosan/biphasic	447:463	arg1	CS/BCP					484:489	CS/BCP	484:489	CS/BCP	484:489	In the present study, we established chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles.					
29790251	2	38	theme	chitosan/biphasic	447:463	arg1	phosphate					473:481	chitosan/biphasic calcium phosphate	447:481	chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles	447:569	In the present study, we established chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles.					
29790251	8	39	theme	bioceramic/biopolymer	1286:1306	arg1	candidates					1459:1468	potential candidates	1449:1468	potential candidates for use in bone tissue engineering	1449:1503	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	8	39	theme	bioceramic/biopolymer	1286:1306	arg1	scaffolds					1308:1316	the bioceramic/biopolymer scaffolds	1282:1316	the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules	1282:1359	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	7	40	theme	in	1076:1077	arg1	tests					1097:1101	in vivo implantation tests	1076:1101	in vivo implantation tests	1076:1101	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	1	41	theme	suitable	245:252	arg1	properties					265:274	suitable mechanical properties	245:274	suitable mechanical properties	245:274	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	7	42	theme	binding	1209:1215	arg1	surface					1217:1223	integrin binding surface	1200:1223	integrin binding surface	1200:1223	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	3	43	theme	structural	687:696	arg1	properties					698:707	structural properties	687:707	structural properties	687:707	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	1	44	theme	bone	146:149	arg1	engineering					158:168	bone tissue engineering	146:168	bone tissue engineering	146:168	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	0	45	theme	BMP-2-encapsulated	66:83	arg1	nanoparticles					85:97	BMP-2-encapsulated nanoparticles	66:97	BMP-2-encapsulated nanoparticles	66:97	Chitosan/biphasic calcium phosphate scaffolds functionalized with BMP-2-encapsulated nanoparticles and RGD for bone regeneration.					
29790251	6	46	theme	BSA	908:910	arg1	nanoparticles					912:924	The BMP-2-encapsulated BSA nanoparticles	885:924	The BMP-2-encapsulated BSA nanoparticles	885:924	The BMP-2-encapsulated BSA nanoparticles were prepared via a desolvation method and then coated with CS and oxidized alginate to achieve sustained release of BMP-2.					
29790251	1	47	theme	tissue	151:156	arg1	engineering					158:168	bone tissue engineering	146:168	bone tissue engineering	146:168	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	1	48	from	Advancements	130:141	arg1	engineering					158:168	bone tissue engineering	146:168	bone tissue engineering	146:168	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	6	49	theme	oxidized	993:1000	arg1	alginate					1002:1009	oxidized alginate	993:1009	oxidized alginate	993:1009	The BMP-2-encapsulated BSA nanoparticles were prepared via a desolvation method and then coated with CS and oxidized alginate to achieve sustained release of BMP-2.					
29790251	7	50	theme	implantation	1084:1095	arg1	tests					1097:1101	in vivo implantation tests	1076:1101	in vivo implantation tests	1076:1101	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	0	51	dep	scaffolds	36:44	arg1	functionalized					46:59	functionalized	46:59	scaffolds functionalized with BMP-2-encapsulated nanoparticles and RGD for bone regeneration	36:127	Chitosan/biphasic calcium phosphate scaffolds functionalized with BMP-2-encapsulated nanoparticles and RGD for bone regeneration.					
29790251	1	52	dep	exhibit	237:243	arg1	should					221:226	should	221:226	should	221:226	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	1	53	theme	mechanical	254:263	arg1	properties					265:274	suitable mechanical properties	245:274	suitable mechanical properties	245:274	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	8	54	theme	bone	1481:1484	arg1	engineering					1493:1503	bone tissue engineering	1481:1503	bone tissue engineering	1481:1503	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	6	55	theme	BMP-2-encapsulated	889:906	arg1	nanoparticles					912:924	The BMP-2-encapsulated BSA nanoparticles	885:924	The BMP-2-encapsulated BSA nanoparticles	885:924	The BMP-2-encapsulated BSA nanoparticles were prepared via a desolvation method and then coated with CS and oxidized alginate to achieve sustained release of BMP-2.					
29790251	3	56	theme	composition	671:681	arg1	terms					662:666	terms	662:666	terms of composition and structural properties	662:707	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	4	57	theme	CS/BCP	732:737	arg1	scaffolds					756:764	CS/BCP composite bionic scaffolds	732:764	CS/BCP composite bionic scaffolds	732:764	First, we synthesized CS/BCP composite bionic scaffolds via the freeze-drying method.					
29790251	10	58	dep	Res	1552:1554	arg1	106A					1564:1567	106A	1564:1567	106A	1564:1567	J Biomed Mater Res Part A: 106A: 2613-2624, 2018.					
29790251	10	58	dep	Res	1552:1554	arg1	A					1561:1561	Part A	1556:1561	J Biomed Mater Res Part A: 106A: 2613-2624, 2018.	1537:1585	J Biomed Mater Res Part A: 106A: 2613-2624, 2018.					
29790251	2	59	theme	BMP-2-loaded	544:555	arg1	nanoparticles					557:569	BMP-2-loaded nanoparticles	544:569	BMP-2-loaded nanoparticles	544:569	In the present study, we established chitosan/biphasic calcium phosphate (CS/BCP) scaffolds functionalized with Arg-Gly-Asp (RGD) and BMP-2-loaded nanoparticles.					
29790251	7	60	theme	cell	1059:1062	arg1	culture					1064:1070	In vitro cell culture	1050:1070	In vitro cell culture	1050:1070	In vitro cell culture and in vivo implantation tests confirmed that RGD and BMP-2 synergistically enhanced cell attachment and spreading by providing integrin binding surface and facilitating osteogenic differentiation.					
29790251	4	61	theme	composite	739:747	arg1	scaffolds					756:764	CS/BCP composite bionic scaffolds	732:764	CS/BCP composite bionic scaffolds	732:764	First, we synthesized CS/BCP composite bionic scaffolds via the freeze-drying method.					
29790251	8	62	from	use	1474:1476	arg1	engineering					1493:1503	bone tissue engineering	1481:1503	bone tissue engineering	1481:1503	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	8	63	theme	signaling	1338:1346	arg1	biomolecules					1348:1359	signaling biomolecules	1338:1359	signaling biomolecules	1338:1359	In summary, the bioceramic/biopolymer scaffolds functionalized with signaling biomolecules successfully provided a favorable microenvironment for bone formation and thus serve as potential candidates for use in bone tissue engineering.					
29790251	1	64	theme	bone	370:373	arg1	formation					375:383	bone formation	370:383	bone formation	370:383	Advancements in bone tissue engineering require the improvement of tissue scaffolds, which should not only exhibit suitable mechanical properties and highly porous structures, but also effectively carry signaling molecules that can mediate bone formation and tissue regeneration.					
29790251	3	65	theme	resulting	576:584	arg1	scaffolds					586:594	The resulting scaffolds	572:594	The resulting scaffolds	572:594	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
29790251	3	65	theme	resulting	576:584	arg1	similar					608:614	similar	608:614	similar	608:614	The resulting scaffolds were highly similar to natural bone extracellular matrix (ECM) in terms of composition and structural properties.					
30782517	6	0	theme	obtained	991:998	arg1	multi-functionality					1000:1018	The obtained multi-functionality	987:1018	The obtained multi-functionality on the CL	987:1028	The obtained multi-functionality on the CL did not affect their geometry and refractive properties.					
30782517	1	1	theme	conditions	257:266	arg1	causative					229:237	causative	229:237	causative	229:237	Contact lenses (CL) provide visual correction but their use may also induce several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use.					
30782517	0	2	theme	associated	91:100	arg1	conditions					102:111	associated conditions	91:111	associated conditions	91:111	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact lenses associated conditions and discomfort.					
30782517	2	3	theme	wetting	356:362	arg1	impairment					365:374	insufficient wetting, impairment	343:374	impairment	365:374	Discomfort is mainly caused by insufficient wetting, impairment of the antioxidant defence system and eye infections.					
30782517	4	4	with	CL	742:743	arg1	properties					764:773	antimicrobial properties	750:773	antimicrobial properties	750:773	GA and CS are expected to improve the comfort of CL by imparting respectively antioxidant properties and enhanced wettability, while their combination with ZnO NPs provides the CL with antimicrobial properties.					
30782517	3	5	theme	gallic	547:552	arg1	GA					560:561	GA	560:561	GA	560:561	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	3	5	theme	gallic	547:552	arg1	acid					554:557	gallic acid	547:557	gallic acid (GA)	547:562	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	5	6	theme	logs	851:854	arg1	reduction					856:864	>4.5 logs reduction	846:864	>4.5 logs reduction	846:864	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	5	6	theme	logs	851:854	arg1	efficiency					834:843	high antibacterial efficiency	815:843	high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions	815:918	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	2	7	dep	wetting	356:362	arg1	insufficient					343:354	insufficient	343:354	insufficient	343:354	Discomfort is mainly caused by insufficient wetting, impairment of the antioxidant defence system and eye infections.					
30782517	5	8	theme	high	815:818	arg1	reduction					856:864	>4.5 logs reduction	846:864	>4.5 logs reduction	846:864	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	5	8	theme	high	815:818	arg1	efficiency					834:843	high antibacterial efficiency	815:843	high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions	815:918	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	5	9	theme	composite	788:796	arg1	coating					798:804	The ternary composite coating	776:804	The ternary composite coating	776:804	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	2	10	theme	infections	418:427	arg1	impairment					365:374	insufficient wetting, impairment	343:374	impairment	365:374	Discomfort is mainly caused by insufficient wetting, impairment of the antioxidant defence system and eye infections.					
30782517	5	11	theme	good	935:938	arg1	%					961:961	>72%	958:961	>72%	958:961	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	5	11	theme	good	935:938	arg1	biocompatibility					940:955	good biocompatibility	935:955	good biocompatibility (>72%) with human cell lines	935:984	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	2	12	theme	system	403:408	arg1	impairment					365:374	insufficient wetting, impairment	343:374	impairment	365:374	Discomfort is mainly caused by insufficient wetting, impairment of the antioxidant defence system and eye infections.					
30782517	4	13	theme	ZnO	721:723	arg1	NPs					725:727	ZnO NPs	721:727	ZnO NPs	721:727	GA and CS are expected to improve the comfort of CL by imparting respectively antioxidant properties and enhanced wettability, while their combination with ZnO NPs provides the CL with antimicrobial properties.					
30782517	2	14	theme	defence	395:401	arg1	system					403:408	the antioxidant defence system	379:408	the antioxidant defence system	379:408	Discomfort is mainly caused by insufficient wetting, impairment of the antioxidant defence system and eye infections.					
30782517	0	15	theme	ZnO	16:18	arg1	nanocoating					52:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating	0:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact	0:82	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact lenses associated conditions and discomfort.					
30782517	4	16	with	combination	704:714	arg1	NPs					725:727	ZnO NPs	721:727	ZnO NPs	721:727	GA and CS are expected to improve the comfort of CL by imparting respectively antioxidant properties and enhanced wettability, while their combination with ZnO NPs provides the CL with antimicrobial properties.					
30782517	2	17	theme	antioxidant	383:393	arg1	system					403:408	the antioxidant defence system	379:408	the antioxidant defence system	379:408	Discomfort is mainly caused by insufficient wetting, impairment of the antioxidant defence system and eye infections.					
30782517	0	18	theme	Multifunctional	0:14	arg1	nanocoating					52:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating	0:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact	0:82	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact lenses associated conditions and discomfort.					
30782517	3	19	with	CL	496:497	arg1	GA					560:561	GA	560:561	GA	560:561	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	3	19	with	CL	496:497	arg1	nanoparticles					508:520	ZnO nanoparticles	504:520	ZnO nanoparticles (NPs)	504:526	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	3	19	with	CL	496:497	arg1	CS					539:540	CS	539:540	CS	539:540	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	3	19	with	CL	496:497	arg1	NPs					523:525	NPs	523:525	NPs	523:525	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	3	19	with	CL	496:497	arg1	chitosan					529:536	chitosan	529:536	chitosan (CS)	529:541	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	3	19	with	CL	496:497	arg1	acid					554:557	gallic acid	547:557	gallic acid (GA)	547:562	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	6	20	from	multi-functionality	1000:1018	arg1	CL					1027:1028	the CL	1023:1028	the CL	1023:1028	The obtained multi-functionality on the CL did not affect their geometry and refractive properties.					
30782517	5	21	theme	CL-related	898:907	arg1	conditions					909:918	CL-related conditions	898:918	CL-related conditions	898:918	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	3	22	theme	current	434:440	arg1	work					442:445	The current work	430:445	The current work	430:445	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	0	23	theme	acid	40:43	arg1	nanocoating					52:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating	0:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact	0:82	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact lenses associated conditions and discomfort.					
30782517	5	24	theme	conditions	909:918	arg1	causative					885:893	causative	885:893	causative	885:893	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	3	25	theme	single	460:465	arg1	coating					485:491	a single step sonochemical coating	458:491	a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA)	458:562	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	0	26	theme	NPs-chitosan-gallic	20:38	arg1	nanocoating					52:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating	0:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact	0:82	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact lenses associated conditions and discomfort.					
30782517	1	27	theme	Contact	129:135	arg1	CL					145:146	CL	145:146	CL	145:146	Contact lenses (CL) provide visual correction but their use may also induce several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use.					
30782517	1	27	theme	Contact	129:135	arg1	lenses					137:142	Contact lenses	129:142	Contact lenses (CL)	129:147	Contact lenses (CL) provide visual correction but their use may also induce several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use.					
30782517	5	28	theme	cell	975:978	arg1	lines					980:984	human cell lines	969:984	human cell lines	969:984	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	1	29	theme	several	205:211	arg1	effects					221:227	several adverse effects	205:227	several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use	205:309	Contact lenses (CL) provide visual correction but their use may also induce several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use.					
30782517	4	30	theme	antioxidant	643:653	arg1	properties					655:664	antioxidant properties	643:664	antioxidant properties	643:664	GA and CS are expected to improve the comfort of CL by imparting respectively antioxidant properties and enhanced wettability, while their combination with ZnO NPs provides the CL with antimicrobial properties.					
30782517	1	31	theme	adverse	213:219	arg1	effects					221:227	several adverse effects	205:227	several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use	205:309	Contact lenses (CL) provide visual correction but their use may also induce several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use.					
30782517	2	32	theme	eye	414:416	arg1	infections					418:427	eye infections	414:427	eye infections	414:427	Discomfort is mainly caused by insufficient wetting, impairment of the antioxidant defence system and eye infections.					
30782517	0	33	theme	hybrid	45:50	arg1	nanocoating					52:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating	0:62	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact	0:82	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact lenses associated conditions and discomfort.					
30782517	5	34	with	biocompatibility	940:955	arg1	lines					980:984	human cell lines	969:984	human cell lines	969:984	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	4	35	theme	antimicrobial	750:762	arg1	properties					764:773	antimicrobial properties	750:773	antimicrobial properties	750:773	GA and CS are expected to improve the comfort of CL by imparting respectively antioxidant properties and enhanced wettability, while their combination with ZnO NPs provides the CL with antimicrobial properties.					
30782517	0	36	dep	nanocoating	52:62	arg1	overcome					67:74	overcome	67:74	to overcome contact	64:82	Multifunctional ZnO NPs-chitosan-gallic acid hybrid nanocoating to overcome contact lenses associated conditions and discomfort.					
30782517	3	37	theme	step	467:470	arg1	coating					485:491	a single step sonochemical coating	458:491	a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA)	458:562	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	3	38	theme	ZnO	504:506	arg1	NPs					523:525	NPs	523:525	NPs	523:525	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	3	38	theme	ZnO	504:506	arg1	nanoparticles					508:520	ZnO nanoparticles	504:520	ZnO nanoparticles (NPs)	504:526	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	3	39	theme	CL	496:497	arg1	coating					485:491	a single step sonochemical coating	458:491	a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA)	458:562	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	5	40	theme	human	969:973	arg1	lines					980:984	human cell lines	969:984	human cell lines	969:984	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	3	41	theme	sonochemical	472:483	arg1	coating					485:491	a single step sonochemical coating	458:491	a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA)	458:562	The current work reports on a single step sonochemical coating of CL with ZnO nanoparticles (NPs), chitosan (CS) and gallic acid (GA).					
30782517	1	42	theme	causative	229:237	arg1	effects					221:227	several adverse effects	205:227	several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use	205:309	Contact lenses (CL) provide visual correction but their use may also induce several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use.					
30782517	4	43	theme	CL	614:615	arg1	comfort					603:609	the comfort	599:609	the comfort of CL	599:615	GA and CS are expected to improve the comfort of CL by imparting respectively antioxidant properties and enhanced wettability, while their combination with ZnO NPs provides the CL with antimicrobial properties.					
30782517	6	44	theme	refractive	1064:1073	arg1	properties					1075:1084	their geometry and refractive properties	1045:1084	their geometry and refractive properties	1045:1084	The obtained multi-functionality on the CL did not affect their geometry and refractive properties.					
30782517	5	45	theme	ternary	780:786	arg1	coating					798:804	The ternary composite coating	776:804	The ternary composite coating	776:804	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	6	46	theme	geometry	1051:1058	arg1	properties					1075:1084	their geometry and refractive properties	1045:1084	their geometry and refractive properties	1045:1084	The obtained multi-functionality on the CL did not affect their geometry and refractive properties.					
30782517	5	47	theme	antibacterial	820:832	arg1	reduction					856:864	>4.5 logs reduction	846:864	>4.5 logs reduction	846:864	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	5	47	theme	antibacterial	820:832	arg1	efficiency					834:843	high antibacterial efficiency	815:843	high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions	815:918	The ternary composite coating presents high antibacterial efficiency (>4.5 logs reduction) against S. aureus causative of CL-related conditions, and maintains good biocompatibility (>72%) with human cell lines.					
30782517	1	48	theme	discomfort	242:251	arg1	causative					229:237	causative	229:237	causative	229:237	Contact lenses (CL) provide visual correction but their use may also induce several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use.					
30782517	1	49	theme	visual	157:162	arg1	correction					164:173	visual correction	157:173	visual correction	157:173	Contact lenses (CL) provide visual correction but their use may also induce several adverse effects causative of discomfort and conditions that lead to stop or discontinue their use.					
30826403	4	0	theme	conditions	792:801	arg1	effects					756:762	the interaction effects	740:762	the interaction effects of main factors and optimum conditions of process	740:812	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	8	1	located	present	1395:1401	arg1	surface					1410:1416	the surface	1406:1416	the surface of nanocomposite after adsorption	1406:1450	It was confirmed from XPS and EDS analyses that heavy metal ions were present on the surface of nanocomposite after adsorption.					
30826403	8	1	located	present	1395:1401	arg2	ions					1385:1388	heavy metal ions	1373:1388	heavy metal ions	1373:1388	It was confirmed from XPS and EDS analyses that heavy metal ions were present on the surface of nanocomposite after adsorption.					
30826403	7	2	theme	pH	1127:1128	arg1	level					1114:1118	The optimum level	1102:1118	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%)	1102:1245	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	2	theme	pH	1127:1128	arg1	6.5					1264:1266	6.5	1264:1266	6.5	1264:1266	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	13	3	from	effects	1973:1979	arg1	adsorption					2011:2020	the adsorption	2007:2020	the adsorption of Cu(II)	2007:2030	The effects of coexist cation ions on the adsorption of Cu(II) was also investigated under optimal condition.					
30826403	3	4	theme	composite	629:637	arg1	CCD					647:649	CCD	647:649	CCD	647:649	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	4	theme	composite	629:637	arg1	design					639:644	a central composite design	619:644	a central composite design (CCD)	619:650	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	1	5	from	solution	299:306	arg1	removal					268:274	the removal	264:274	the removal of Cu(II) from aqueous solution	264:306	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	4	6	theme	process	806:812	arg1	factors					772:778	main factors	767:778	main factors	767:778	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	4	6	theme	process	806:812	arg1	conditions					792:801	optimum conditions	784:801	optimum conditions	784:801	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	3	7	theme	contact	532:538	arg1	time					540:543	contact time	532:543	contact time	532:543	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	8	theme	response	655:662	arg1	RSM					685:687	RSM	685:687	RSM	685:687	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	8	theme	response	655:662	arg1	methodology					672:682	response surface methodology	655:682	response surface methodology (RSM)	655:688	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	9	theme	effective	497:505	arg1	temperature					579:589	temperature	579:589	temperature	579:589	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	9	theme	effective	497:505	arg1	parameters					507:516	five effective parameters	492:516	five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage	492:611	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	9	theme	effective	497:505	arg1	pH					528:529	pH	528:529	pH	528:529	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	9	theme	effective	497:505	arg1	concentration					554:566	initial concentration	546:566	initial concentration of copper	546:576	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	9	theme	effective	497:505	arg1	dosage					606:611	adsorbent dosage	596:611	adsorbent dosage	596:611	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	9	theme	effective	497:505	arg1	time					540:543	contact time	532:543	contact time	532:543	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	4	10	theme	main	767:770	arg1	factors					772:778	main factors	767:778	main factors	767:778	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	1	11	theme	embedded	164:171	arg1	chitosan					155:162	Poly aniline modified chitosan	133:162	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites	133:201	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	12	12	theme	recycling	1831:1839	arg1	experiments					1841:1851	Further recycling experiments	1823:1851	Further recycling experiments	1823:1851	Further recycling experiments show that nanocomposite still retains 95% of the original adsorption following the 5th adsorption-desorption cycle.					
30826403	4	13	used	used	722:725	arg2	plots					711:715	surface plots	703:715	surface plots	703:715	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	4	13	used	used	722:725	arg2	Contour					691:697	Contour	691:697	Contour	691:697	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	1	14	theme	ZnO/Fe3O4	178:186	arg1	nanocomposites					188:201	ZnO/Fe3O4 nanocomposites	178:201	ZnO/Fe3O4 nanocomposites	178:201	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	9	15	theme	Langmuir	1506:1513	arg1	model					1524:1528	the Langmuir isotherm model	1502:1528	the Langmuir isotherm model	1502:1528	The adsorption equilibrium data fitted well with the Langmuir isotherm model and the adsorption process followed the pseudo-second-order and intra-particle diffusion kinetic model.					
30826403	4	16	theme	optimum	784:790	arg1	conditions					792:801	optimum conditions	784:801	optimum conditions	784:801	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	13	17	theme	cation	1992:1997	arg1	ions					1999:2002	coexist cation ions	1984:2002	coexist cation ions	1984:2002	The effects of coexist cation ions on the adsorption of Cu(II) was also investigated under optimal condition.					
30826403	0	18	theme	aqueous	115:121	arg1	solution					123:130	aqueous solution	115:130	aqueous solution	115:130	Preparation and characterization of poly aniline modified chitosan embedded with ZnO-Fe3O4 for Cu(II) removal from aqueous solution.					
30826403	10	19	theme	adsorption	1648:1657	arg1	328.4 mg/g					1683:1692	328.4 mg/g	1683:1692	328.4 mg/g	1683:1692	The saturated adsorption capacity is found to be 328.4 mg/g.					
30826403	10	19	theme	adsorption	1648:1657	arg1	capacity					1659:1666	The saturated adsorption capacity	1634:1666	The saturated adsorption capacity	1634:1666	The saturated adsorption capacity is found to be 328.4 mg/g.					
30826403	12	20	theme	adsorption-desorption	1940:1960	arg1	cycle					1962:1966	the 5th adsorption-desorption cycle	1932:1966	the 5th adsorption-desorption cycle	1932:1966	Further recycling experiments show that nanocomposite still retains 95% of the original adsorption following the 5th adsorption-desorption cycle.					
30826403	9	21	theme	equilibrium	1468:1478	arg1	data					1480:1483	The adsorption equilibrium data	1453:1483	The adsorption equilibrium data	1453:1483	The adsorption equilibrium data fitted well with the Langmuir isotherm model and the adsorption process followed the pseudo-second-order and intra-particle diffusion kinetic model.					
30826403	5	22	theme	novel	971:975	arg1	absorbent					977:985	novel absorbent	971:985	novel absorbent	971:985	The regression equation coefficients were calculated and the data confirmed the validity of second-order polynomial equation for the removal of Cu(II) with novel absorbent.					
30826403	7	23	theme	Cu	1223:1224	arg1	removal					1230:1236	maximum Cu(II) removal	1215:1236	maximum Cu(II) removal (94.51%)	1215:1245	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	23	theme	Cu	1223:1224	arg1	%					1244:1244	94.51%	1239:1244	94.51%	1239:1244	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	12	24	theme	adsorption	1911:1920	arg1	%					1893:1893	95%	1891:1893	95% of the original adsorption	1891:1920	Further recycling experiments show that nanocomposite still retains 95% of the original adsorption following the 5th adsorption-desorption cycle.					
30826403	12	24	theme	adsorption	1911:1920	arg1	adsorption					1911:1920	the original adsorption	1898:1920	the original adsorption	1898:1920	Further recycling experiments show that nanocomposite still retains 95% of the original adsorption following the 5th adsorption-desorption cycle.					
30826403	1	25	theme	Poly	133:136	arg1	chitosan					155:162	Poly aniline modified chitosan	133:162	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites	133:201	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	14	26	theme	metal	2176:2180	arg1	ions					2182:2185	hazardous metal ions	2166:2185	hazardous metal ions in wastewater	2166:2199	All the results demonstrate that nanocomposite is a potential recyclable adsorbent for hazardous metal ions in wastewater.					
30826403	1	27	theme	modified	146:153	arg1	chitosan					155:162	Poly aniline modified chitosan	133:162	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites	133:201	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	9	28	theme	diffusion	1609:1617	arg1	model					1627:1631	the pseudo-second-order and intra-particle diffusion kinetic model	1566:1631	the pseudo-second-order and intra-particle diffusion kinetic model	1566:1631	The adsorption equilibrium data fitted well with the Langmuir isotherm model and the adsorption process followed the pseudo-second-order and intra-particle diffusion kinetic model.					
30826403	0	29	theme	Cu	95:96	arg1	removal					102:108	Cu(II) removal	95:108	Cu(II) removal from aqueous solution	95:130	Preparation and characterization of poly aniline modified chitosan embedded with ZnO-Fe3O4 for Cu(II) removal from aqueous solution.					
30826403	6	30	theme	copper	1075:1080	arg1	removal					1082:1088	copper removal	1075:1088	copper removal	1075:1088	Analysis of variance (ANOVA) showed a high coefficient of determination value (R2) for copper removal being 0.99.					
30826403	4	31	theme	surface	703:709	arg1	plots					711:715	surface plots	703:715	surface plots	703:715	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	14	32	theme	recyclable	2141:2150	arg1	nanocomposite					2112:2124	nanocomposite	2112:2124	nanocomposite	2112:2124	All the results demonstrate that nanocomposite is a potential recyclable adsorbent for hazardous metal ions in wastewater.					
30826403	14	32	theme	recyclable	2141:2150	arg1	potential					2131:2139	a potential recyclable adsorbent	2129:2160	a potential recyclable adsorbent for hazardous metal ions in wastewater	2129:2199	All the results demonstrate that nanocomposite is a potential recyclable adsorbent for hazardous metal ions in wastewater.					
30826403	5	33	theme	equation	830:837	arg1	coefficients					839:850	The regression equation coefficients	815:850	The regression equation coefficients	815:850	The regression equation coefficients were calculated and the data confirmed the validity of second-order polynomial equation for the removal of Cu(II) with novel absorbent.					
30826403	5	34	theme	polynomial	920:929	arg1	equation					931:938	second-order polynomial equation	907:938	second-order polynomial equation for the removal of Cu(II) with novel absorbent	907:985	The regression equation coefficients were calculated and the data confirmed the validity of second-order polynomial equation for the removal of Cu(II) with novel absorbent.					
30826403	7	35	theme	maximum	1215:1221	arg1	removal					1230:1236	maximum Cu(II) removal	1215:1236	maximum Cu(II) removal (94.51%)	1215:1245	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	35	theme	maximum	1215:1221	arg1	%					1244:1244	94.51%	1239:1244	94.51%	1239:1244	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	36	theme	time	1206:1209	arg1	level					1114:1118	The optimum level	1102:1118	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%)	1102:1245	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	36	theme	time	1206:1209	arg1	6.5					1264:1266	6.5	1264:1266	6.5	1264:1266	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	8	37	theme	EDS	1355:1357	arg1	analyses					1359:1366	EDS analyses	1355:1366	EDS analyses	1355:1366	It was confirmed from XPS and EDS analyses that heavy metal ions were present on the surface of nanocomposite after adsorption.					
30826403	3	38	theme	adsorbent	596:604	arg1	dosage					606:611	adsorbent dosage	596:611	adsorbent dosage	596:611	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	0	39	theme	poly	36:39	arg1	aniline					41:47	poly aniline	36:47	poly aniline	36:47	Preparation and characterization of poly aniline modified chitosan embedded with ZnO-Fe3O4 for Cu(II) removal from aqueous solution.					
30826403	5	40	theme	Cu	959:960	arg1	removal					948:954	the removal	944:954	the removal of Cu(II) with novel absorbent	944:985	The regression equation coefficients were calculated and the data confirmed the validity of second-order polynomial equation for the removal of Cu(II) with novel absorbent.					
30826403	7	41	theme	adsorbent	1177:1185	arg1	dosage					1187:1192	adsorbent dosage	1177:1192	adsorbent dosage	1177:1192	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	2	42	theme	zeta-potential	402:415	arg1	analyses					417:424	zeta-potential analyses	402:424	zeta-potential analyses	402:424	The synthesized nanocomposite was characterized by FT-IR, XRD, FESEM, TEM, EDS, TGA, BET and zeta-potential analyses.					
30826403	8	43	theme	metal	1379:1383	arg1	ions					1385:1388	heavy metal ions	1373:1388	heavy metal ions	1373:1388	It was confirmed from XPS and EDS analyses that heavy metal ions were present on the surface of nanocomposite after adsorption.					
30826403	6	44	dep	showed	1017:1022	arg1	0.99					1096:1099	0.99	1096:1099	0.99	1096:1099	Analysis of variance (ANOVA) showed a high coefficient of determination value (R2) for copper removal being 0.99.					
30826403	11	45	theme	Thermodynamics	1695:1708	arg1	analysis					1710:1717	Thermodynamics analysis	1695:1717	Thermodynamics analysis	1695:1717	Thermodynamics analysis suggests that the adsorption process is endothermic, with increasing entropy and spontaneous in nature.					
30826403	1	46	theme	aqueous	291:297	arg1	solution					299:306	aqueous solution	291:306	aqueous solution	291:306	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	14	47	from	ions	2182:2185	arg1	wastewater					2190:2199	wastewater	2190:2199	wastewater	2190:2199	All the results demonstrate that nanocomposite is a potential recyclable adsorbent for hazardous metal ions in wastewater.					
30826403	7	48	theme	copper	1169:1174	arg1	temperature					1131:1141	temperature	1131:1141	temperature	1131:1141	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	48	theme	copper	1169:1174	arg1	concentration					1152:1164	initial concentration	1144:1164	initial concentration of copper	1144:1174	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	48	theme	copper	1169:1174	arg1	pH					1127:1128	pH	1127:1128	pH	1127:1128	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	48	theme	copper	1169:1174	arg1	dosage					1187:1192	adsorbent dosage	1177:1192	adsorbent dosage	1177:1192	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	48	theme	copper	1169:1174	arg1	time					1206:1209	contact time	1198:1209	contact time	1198:1209	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	3	49	theme	central	621:627	arg1	CCD					647:649	CCD	647:649	CCD	647:649	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	49	theme	central	621:627	arg1	design					639:644	a central composite design	619:644	a central composite design (CCD)	619:650	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	1	50	with	embedded	164:171	arg1	nanocomposites					188:201	ZnO/Fe3O4 nanocomposites	178:201	ZnO/Fe3O4 nanocomposites	178:201	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	7	51	theme	concentration	1152:1164	arg1	level					1114:1118	The optimum level	1102:1118	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%)	1102:1245	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	51	theme	concentration	1152:1164	arg1	6.5					1264:1266	6.5	1264:1266	6.5	1264:1266	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	13	52	dep	ions	1999:2002	arg1	coexist					1984:1990	coexist	1984:1990	coexist	1984:1990	The effects of coexist cation ions on the adsorption of Cu(II) was also investigated under optimal condition.					
30826403	9	53	theme	adsorption	1457:1466	arg1	data					1480:1483	The adsorption equilibrium data	1453:1483	The adsorption equilibrium data	1453:1483	The adsorption equilibrium data fitted well with the Langmuir isotherm model and the adsorption process followed the pseudo-second-order and intra-particle diffusion kinetic model.					
30826403	8	54	theme	nanocomposite	1421:1433	arg1	surface					1410:1416	the surface	1406:1416	the surface of nanocomposite after adsorption	1406:1450	It was confirmed from XPS and EDS analyses that heavy metal ions were present on the surface of nanocomposite after adsorption.					
30826403	13	55	theme	Cu	2025:2026	arg1	adsorption					2011:2020	the adsorption	2007:2020	the adsorption of Cu(II)	2007:2030	The effects of coexist cation ions on the adsorption of Cu(II) was also investigated under optimal condition.					
30826403	5	56	theme	regression	819:828	arg1	coefficients					839:850	The regression equation coefficients	815:850	The regression equation coefficients	815:850	The regression equation coefficients were calculated and the data confirmed the validity of second-order polynomial equation for the removal of Cu(II) with novel absorbent.					
30826403	3	57	theme	surface	664:670	arg1	RSM					685:687	RSM	685:687	RSM	685:687	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	57	theme	surface	664:670	arg1	methodology					672:682	response surface methodology	655:682	response surface methodology (RSM)	655:688	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	0	58	with	embedded	67:74	arg1	ZnO-Fe3O4					81:89	ZnO-Fe3O4	81:89	ZnO-Fe3O4 for Cu(II) removal from aqueous solution	81:130	Preparation and characterization of poly aniline modified chitosan embedded with ZnO-Fe3O4 for Cu(II) removal from aqueous solution.					
30826403	13	59	theme	optimal	2060:2066	arg1	condition					2068:2076	optimal condition	2060:2076	optimal condition	2060:2076	The effects of coexist cation ions on the adsorption of Cu(II) was also investigated under optimal condition.					
30826403	12	60	theme	Further	1823:1829	arg1	experiments					1841:1851	Further recycling experiments	1823:1851	Further recycling experiments	1823:1851	Further recycling experiments show that nanocomposite still retains 95% of the original adsorption following the 5th adsorption-desorption cycle.					
30826403	4	61	theme	factors	772:778	arg1	effects					756:762	the interaction effects	740:762	the interaction effects of main factors and optimum conditions of process	740:812	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	3	62	theme	parameters	507:516	arg1	function					480:487	a function	478:487	a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage	478:611	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	62	theme	parameters	507:516	arg1	experiments					448:458	The adsorption batch experiments	427:458	The adsorption batch experiments	427:458	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	9	63	theme	isotherm	1515:1522	arg1	model					1524:1528	the Langmuir isotherm model	1502:1528	the Langmuir isotherm model	1502:1528	The adsorption equilibrium data fitted well with the Langmuir isotherm model and the adsorption process followed the pseudo-second-order and intra-particle diffusion kinetic model.					
30826403	6	64	theme	determination	1046:1058	arg1	R2					1067:1068	R2	1067:1068	R2	1067:1068	Analysis of variance (ANOVA) showed a high coefficient of determination value (R2) for copper removal being 0.99.					
30826403	6	64	theme	determination	1046:1058	arg1	value					1060:1064	determination value	1046:1064	determination value (R2)	1046:1069	Analysis of variance (ANOVA) showed a high coefficient of determination value (R2) for copper removal being 0.99.					
30826403	13	65	theme	ions	1999:2002	arg1	effects					1973:1979	The effects	1969:1979	The effects of coexist cation ions on the adsorption of Cu(II)	1969:2030	The effects of coexist cation ions on the adsorption of Cu(II) was also investigated under optimal condition.					
30826403	10	66	theme	saturated	1638:1646	arg1	328.4 mg/g					1683:1692	328.4 mg/g	1683:1692	328.4 mg/g	1683:1692	The saturated adsorption capacity is found to be 328.4 mg/g.					
30826403	10	66	theme	saturated	1638:1646	arg1	capacity					1659:1666	The saturated adsorption capacity	1634:1666	The saturated adsorption capacity	1634:1666	The saturated adsorption capacity is found to be 328.4 mg/g.					
30826403	9	67	theme	adsorption	1538:1547	arg1	process					1549:1555	the adsorption process	1534:1555	the adsorption process	1534:1555	The adsorption equilibrium data fitted well with the Langmuir isotherm model and the adsorption process followed the pseudo-second-order and intra-particle diffusion kinetic model.					
30826403	11	68	from	entropy	1788:1794	arg1	nature					1815:1820	nature	1815:1820	nature	1815:1820	Thermodynamics analysis suggests that the adsorption process is endothermic, with increasing entropy and spontaneous in nature.					
30826403	12	69	theme	5th	1936:1938	arg1	cycle					1962:1966	the 5th adsorption-desorption cycle	1932:1966	the 5th adsorption-desorption cycle	1932:1966	Further recycling experiments show that nanocomposite still retains 95% of the original adsorption following the 5th adsorption-desorption cycle.					
30826403	3	70	theme	copper	571:576	arg1	temperature					579:589	temperature	579:589	temperature	579:589	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	70	theme	copper	571:576	arg1	pH					528:529	pH	528:529	pH	528:529	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	70	theme	copper	571:576	arg1	concentration					554:566	initial concentration	546:566	initial concentration of copper	546:576	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	70	theme	copper	571:576	arg1	dosage					606:611	adsorbent dosage	596:611	adsorbent dosage	596:611	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	70	theme	copper	571:576	arg1	time					540:543	contact time	532:543	contact time	532:543	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	6	71	theme	value	1060:1064	arg1	coefficient					1031:1041	a high coefficient	1024:1041	a high coefficient of determination value (R2) for copper removal	1024:1088	Analysis of variance (ANOVA) showed a high coefficient of determination value (R2) for copper removal being 0.99.					
30826403	7	72	theme	initial	1144:1150	arg1	concentration					1152:1164	initial concentration	1144:1164	initial concentration of copper	1144:1174	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	9	73	theme	pseudo-second-order	1570:1588	arg1	model					1627:1631	the pseudo-second-order and intra-particle diffusion kinetic model	1566:1631	the pseudo-second-order and intra-particle diffusion kinetic model	1566:1631	The adsorption equilibrium data fitted well with the Langmuir isotherm model and the adsorption process followed the pseudo-second-order and intra-particle diffusion kinetic model.					
30826403	12	74	theme	original	1902:1909	arg1	adsorption					1911:1920	the original adsorption	1898:1920	the original adsorption	1898:1920	Further recycling experiments show that nanocomposite still retains 95% of the original adsorption following the 5th adsorption-desorption cycle.					
30826403	7	75	theme	optimum	1106:1112	arg1	level					1114:1118	The optimum level	1102:1118	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%)	1102:1245	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	75	theme	optimum	1106:1112	arg1	6.5					1264:1266	6.5	1264:1266	6.5	1264:1266	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	8	76	attach	present	1395:1401	arg1	surface					1410:1416	the surface	1406:1416	the surface of nanocomposite after adsorption	1406:1450	It was confirmed from XPS and EDS analyses that heavy metal ions were present on the surface of nanocomposite after adsorption.					
30826403	8	76	attach	present	1395:1401	arg2	ions					1385:1388	heavy metal ions	1373:1388	heavy metal ions	1373:1388	It was confirmed from XPS and EDS analyses that heavy metal ions were present on the surface of nanocomposite after adsorption.					
30826403	1	77	theme	aniline	138:144	arg1	chitosan					155:162	Poly aniline modified chitosan	133:162	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites	133:201	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	9	78	theme	intra-particle	1594:1607	arg1	model					1627:1631	the pseudo-second-order and intra-particle diffusion kinetic model	1566:1631	the pseudo-second-order and intra-particle diffusion kinetic model	1566:1631	The adsorption equilibrium data fitted well with the Langmuir isotherm model and the adsorption process followed the pseudo-second-order and intra-particle diffusion kinetic model.					
30826403	14	79	theme	hazardous	2166:2174	arg1	ions					2182:2185	hazardous metal ions	2166:2185	hazardous metal ions in wastewater	2166:2199	All the results demonstrate that nanocomposite is a potential recyclable adsorbent for hazardous metal ions in wastewater.					
30826403	9	80	theme	kinetic	1619:1625	arg1	model					1627:1631	the pseudo-second-order and intra-particle diffusion kinetic model	1566:1631	the pseudo-second-order and intra-particle diffusion kinetic model	1566:1631	The adsorption equilibrium data fitted well with the Langmuir isotherm model and the adsorption process followed the pseudo-second-order and intra-particle diffusion kinetic model.					
30826403	14	81	theme	adsorbent	2152:2160	arg1	nanocomposite					2112:2124	nanocomposite	2112:2124	nanocomposite	2112:2124	All the results demonstrate that nanocomposite is a potential recyclable adsorbent for hazardous metal ions in wastewater.					
30826403	14	81	theme	adsorbent	2152:2160	arg1	potential					2131:2139	a potential recyclable adsorbent	2129:2160	a potential recyclable adsorbent for hazardous metal ions in wastewater	2129:2199	All the results demonstrate that nanocomposite is a potential recyclable adsorbent for hazardous metal ions in wastewater.					
30826403	5	82	theme	second-order	907:918	arg1	equation					931:938	second-order polynomial equation	907:938	second-order polynomial equation for the removal of Cu(II) with novel absorbent	907:985	The regression equation coefficients were calculated and the data confirmed the validity of second-order polynomial equation for the removal of Cu(II) with novel absorbent.					
30826403	6	83	theme	variance	1000:1007	arg1	Analysis					988:995	Analysis	988:995	Analysis of variance (ANOVA)	988:1015	Analysis of variance (ANOVA) showed a high coefficient of determination value (R2) for copper removal being 0.99.					
30826403	3	84	theme	batch	442:446	arg1	function					480:487	a function	478:487	a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage	478:611	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	84	theme	batch	442:446	arg1	experiments					448:458	The adsorption batch experiments	427:458	The adsorption batch experiments	427:458	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	7	85	dep	pH	1127:1128	arg1	the					1123:1125	the	1123:1125	the	1123:1125	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	86	theme	contact	1198:1204	arg1	time					1206:1209	contact time	1198:1209	contact time	1198:1209	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	1	87	theme	Cu	279:280	arg1	removal					268:274	the removal	264:274	the removal of Cu(II) from aqueous solution	264:306	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	7	88	theme	dosage	1187:1192	arg1	level					1114:1118	The optimum level	1102:1118	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%)	1102:1245	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	88	theme	dosage	1187:1192	arg1	6.5					1264:1266	6.5	1264:1266	6.5	1264:1266	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	8	89	theme	heavy	1373:1377	arg1	ions					1385:1388	heavy metal ions	1373:1388	heavy metal ions	1373:1388	It was confirmed from XPS and EDS analyses that heavy metal ions were present on the surface of nanocomposite after adsorption.					
30826403	0	90	theme	aniline	41:47	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of poly aniline modified chitosan embedded with ZnO-Fe3O4 for Cu(II) removal from aqueous solution.					
30826403	0	90	theme	aniline	41:47	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of poly aniline modified chitosan embedded with ZnO-Fe3O4 for Cu(II) removal from aqueous solution.					
30826403	4	91	theme	interaction	744:754	arg1	effects					756:762	the interaction effects	740:762	the interaction effects of main factors and optimum conditions of process	740:812	Contour and surface plots were used to determine the interaction effects of main factors and optimum conditions of process.					
30826403	2	92	theme	synthesized	313:323	arg1	nanocomposite					325:337	The synthesized nanocomposite	309:337	The synthesized nanocomposite	309:337	The synthesized nanocomposite was characterized by FT-IR, XRD, FESEM, TEM, EDS, TGA, BET and zeta-potential analyses.					
30826403	5	93	theme	equation	931:938	arg1	validity					895:902	the validity	891:902	the validity of second-order polynomial equation for the removal of Cu(II) with novel absorbent	891:985	The regression equation coefficients were calculated and the data confirmed the validity of second-order polynomial equation for the removal of Cu(II) with novel absorbent.					
30826403	3	94	theme	initial	546:552	arg1	concentration					554:566	initial concentration	546:566	initial concentration of copper	546:576	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	6	95	theme	high	1026:1029	arg1	coefficient					1031:1041	a high coefficient	1024:1041	a high coefficient of determination value (R2) for copper removal	1024:1088	Analysis of variance (ANOVA) showed a high coefficient of determination value (R2) for copper removal being 0.99.					
30826403	0	96	from	solution	123:130	arg1	removal					102:108	Cu(II) removal	95:108	Cu(II) removal from aqueous solution	95:130	Preparation and characterization of poly aniline modified chitosan embedded with ZnO-Fe3O4 for Cu(II) removal from aqueous solution.					
30826403	3	97	theme	adsorption	431:440	arg1	function					480:487	a function	478:487	a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage	478:611	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	3	97	theme	adsorption	431:440	arg1	experiments					448:458	The adsorption batch experiments	427:458	The adsorption batch experiments	427:458	The adsorption batch experiments were conducted as a function of five effective parameters including pH, contact time, initial concentration of copper, temperature, and adsorbent dosage using a central composite design (CCD) in response surface methodology (RSM).					
30826403	1	98	theme	precipitation	228:240	arg1	method					242:247	a precipitation method	226:247	a precipitation method	226:247	Poly aniline modified chitosan embedded with ZnO/Fe3O4 nanocomposites were synthesized using a precipitation method and applied to the removal of Cu(II) from aqueous solution.					
30826403	7	99	theme	temperature	1131:1141	arg1	level					1114:1118	The optimum level	1102:1118	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%)	1102:1245	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	7	99	theme	temperature	1131:1141	arg1	6.5					1264:1266	6.5	1264:1266	6.5	1264:1266	The optimum level of the pH, temperature, initial concentration of copper, adsorbent dosage and contact time for maximum Cu(II) removal (94.51%) were found to be 6.5, 31 °C, 82 mg L-1, 0.81 g L-1, and 51 min, respectively.					
30826403	5	100	with	removal	948:954	arg1	absorbent					977:985	novel absorbent	971:985	novel absorbent	971:985	The regression equation coefficients were calculated and the data confirmed the validity of second-order polynomial equation for the removal of Cu(II) with novel absorbent.					
30826403	11	101	theme	adsorption	1737:1746	arg1	endothermic					1759:1769	endothermic	1759:1769	endothermic	1759:1769	Thermodynamics analysis suggests that the adsorption process is endothermic, with increasing entropy and spontaneous in nature.					
30826403	11	101	theme	adsorption	1737:1746	arg1	process					1748:1754	the adsorption process	1733:1754	the adsorption process	1733:1754	Thermodynamics analysis suggests that the adsorption process is endothermic, with increasing entropy and spontaneous in nature.					
29642399	0	0	theme	Oil	73:75	arg1	Activity					14:21	Antibacterial Activity	0:21	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.	0:149	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	5	1	theme	synergetic	1040:1049	arg1	effect					1065:1070	the strongest synergetic antibacterial effect	1026:1070	the strongest synergetic antibacterial effect on S. aureus and E. coli	1026:1095	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	7	2	theme	natural	1335:1341	arg1	preservative					1348:1359	a novel natural food preservative	1327:1359	a novel natural food preservative	1327:1359	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	7	3	theme	value	1423:1427	arg1	processability					1375:1388	the processability	1371:1388	the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan,	1371:1486	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	0	4	theme	Peel	68:71	arg1	Oil					73:75	Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil	26:75	Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil	26:75	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	0	5	from	Activity	14:21	arg1	aureus					122:127	Staphylococcus aureus	107:127	Staphylococcus aureus	107:127	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	0	5	from	Activity	14:21	arg1	coli					145:148	Escherichia coli	133:148	Escherichia coli	133:148	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	4	6	theme	inhibitory	812:821	arg1	concentration					823:835	a minimal inhibitory concentration	802:835	a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization	802:932	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	4	6	theme	inhibitory	812:821	arg1	times					868:872	times	868:872	times	868:872	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	4	6	theme	inhibitory	812:821	arg1	MIC					838:840	MIC	838:840	MIC	838:840	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	5	7	theme	oil	976:978	arg1	emulsion					980:987	0.4% essential oil emulsion	961:987	0.4% essential oil emulsion	961:987	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	5	8	theme	emulsion	980:987	arg1	mixture					950:956	a mixture	948:956	a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan	948:1020	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	0	9	theme	Water-Soluble	81:93	arg1	Chitosan					95:102	Water-Soluble Chitosan	81:102	Water-Soluble Chitosan	81:102	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	6	10	theme	mixture	1165:1171	arg1	value					1151:1155	the MIC value	1143:1155	the MIC value of this mixture	1143:1171	In comparison with chitosan alone, the MIC value of this mixture was significantly 2.4 and 2.5 times lower.					
29642399	6	10	theme	mixture	1165:1171	arg1	lower					1209:1213	lower	1209:1213	lower	1209:1213	In comparison with chitosan alone, the MIC value of this mixture was significantly 2.4 and 2.5 times lower.					
29642399	6	11	theme	MIC	1147:1149	arg1	value					1151:1155	the MIC value	1143:1155	the MIC value of this mixture	1143:1171	In comparison with chitosan alone, the MIC value of this mixture was significantly 2.4 and 2.5 times lower.					
29642399	6	11	theme	MIC	1147:1149	arg1	lower					1209:1213	lower	1209:1213	lower	1209:1213	In comparison with chitosan alone, the MIC value of this mixture was significantly 2.4 and 2.5 times lower.					
29642399	4	12	theme	pomelo	720:725	arg1	oil					732:734	The pomelo peel oil	716:734	The pomelo peel oil	716:734	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	6	13	with	comparison	1111:1120	arg1	chitosan					1127:1134	chitosan	1127:1134	chitosan	1127:1134	In comparison with chitosan alone, the MIC value of this mixture was significantly 2.4 and 2.5 times lower.					
29642399	5	14	contain	had	1022:1024	arg2	effect					1065:1070	the strongest synergetic antibacterial effect	1026:1070	the strongest synergetic antibacterial effect on S. aureus and E. coli	1026:1095	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	5	14	contain	had	1022:1024	arg1	mixture					950:956	a mixture	948:956	a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan	948:1020	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	5	15	theme	water-soluble	999:1011	arg1	chitosan					1013:1020	0.03% water-soluble chitosan	993:1020	0.03% water-soluble chitosan	993:1020	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	2	16	from	activity	347:354	arg1	conditions					418:427	different homogenizer speed conditions and concentrations	390:446	conditions	418:427	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	16	from	activity	347:354	arg1	concentrations					433:446	different homogenizer speed conditions and concentrations	390:446	concentrations	433:446	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	17	theme	chromatography-mass	283:301	arg1	GC-MS					317:321	GC-MS	317:321	GC-MS	317:321	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	17	theme	chromatography-mass	283:301	arg1	spectrometry					303:314	gas chromatography-mass spectrometry	279:314	gas chromatography-mass spectrometry (GC-MS)	279:322	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	7	18	theme	oil	1285:1287	arg1	mixture					1251:1257	a mixture	1249:1257	a mixture of emulsified pomelo peel oil and water-soluble chitosan	1249:1314	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	5	19	theme	chitosan	1013:1020	arg1	mixture					950:956	a mixture	948:956	a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan	948:1020	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	7	20	theme	emulsified	1262:1271	arg1	oil					1285:1287	emulsified pomelo peel oil	1262:1287	emulsified pomelo peel oil	1262:1287	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	2	21	theme	gas	279:281	arg1	GC-MS					317:321	GC-MS	317:321	GC-MS	317:321	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	21	theme	gas	279:281	arg1	spectrometry					303:314	gas chromatography-mass spectrometry	279:314	gas chromatography-mass spectrometry (GC-MS)	279:322	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	22	theme	homogenizer	400:410	arg1	conditions					418:427	different homogenizer speed conditions and concentrations	390:446	conditions	418:427	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	0	23	theme	Chitosan	95:102	arg1	Activity					14:21	Antibacterial Activity	0:21	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.	0:149	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	2	24	theme	chitosan	465:472	arg1	conditions					418:427	different homogenizer speed conditions and concentrations	390:446	conditions	418:427	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	24	theme	chitosan	465:472	arg1	concentrations					433:446	different homogenizer speed conditions and concentrations	390:446	concentrations	433:446	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	1	25	theme	pomelo	171:176	arg1	distillation					184:195	pomelo steam distillation	171:195	pomelo steam distillation	171:195	This study utilized pomelo steam distillation to isolate pomelo peel essential oil.					
29642399	7	26	theme	novel	1329:1333	arg1	preservative					1348:1359	a novel natural food preservative	1327:1359	a novel natural food preservative	1327:1359	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	4	27	theme	minimal	804:810	arg1	concentration					823:835	a minimal inhibitory concentration	802:835	a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization	802:932	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	4	27	theme	minimal	804:810	arg1	times					868:872	times	868:872	times	868:872	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	4	27	theme	minimal	804:810	arg1	MIC					838:840	MIC	838:840	MIC	838:840	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	7	28	theme	chitosan	1307:1314	arg1	mixture					1251:1257	a mixture	1249:1257	a mixture of emulsified pomelo peel oil and water-soluble chitosan	1249:1314	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	7	29	theme	food	1343:1346	arg1	preservative					1348:1359	a novel natural food preservative	1327:1359	a novel natural food preservative	1327:1359	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	1	30	theme	steam	178:182	arg1	distillation					184:195	pomelo steam distillation	171:195	pomelo steam distillation	171:195	This study utilized pomelo steam distillation to isolate pomelo peel essential oil.					
29642399	0	31	theme	Antibacterial	0:12	arg1	Activity					14:21	Antibacterial Activity	0:21	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.	0:149	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	3	32	theme	total	709:713	arg1	total					709:713	total	709:713	total	709:713	Analysis of the essential oil composition identified a total of 33 compounds with the main constituent, limonene accounting for 87.5% (940.07 mg/g) of the total.					
29642399	3	32	theme	total	709:713	arg1	%					686:686	87.5%	682:686	87.5% (940.07 mg/g) of the total	682:713	Analysis of the essential oil composition identified a total of 33 compounds with the main constituent, limonene accounting for 87.5% (940.07 mg/g) of the total.					
29642399	3	32	theme	total	709:713	arg1	mg/g					696:699	940.07 mg/g	689:699	940.07 mg/g	689:699	Analysis of the essential oil composition identified a total of 33 compounds with the main constituent, limonene accounting for 87.5% (940.07 mg/g) of the total.					
29642399	2	33	dep	chitosan	465:472	arg1	=					501:501	=	501:501	=	501:501	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	34	theme	emulsions	377:385	arg1	activity					347:354	the antibacterial activity	329:354	the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli	329:538	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	0	35	theme	Emulsified	26:35	arg1	Osbeck					60:65	Citrus grandis Osbeck	45:65	Citrus grandis Osbeck	45:65	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	0	35	theme	Emulsified	26:35	arg1	Pomelo					37:42	Emulsified Pomelo	26:42	Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil	26:75	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	5	36	theme	%	997:997	arg1	chitosan					1013:1020	0.03% water-soluble chitosan	993:1020	0.03% water-soluble chitosan	993:1020	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	7	37	theme	pomelo	1273:1278	arg1	oil					1285:1287	emulsified pomelo peel oil	1262:1287	emulsified pomelo peel oil	1262:1287	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	7	38	theme	chitosan	1478:1485	arg1	byproducts					1436:1445	the byproducts	1432:1445	the byproducts of the Taiwan Matou pomelo and chitosan	1432:1485	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	7	39	theme	water-soluble	1293:1305	arg1	chitosan					1307:1314	water-soluble chitosan	1293:1314	water-soluble chitosan	1293:1314	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	4	40	theme	1.9	864:866	arg1	concentration					823:835	a minimal inhibitory concentration	802:835	a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization	802:932	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	4	40	theme	1.9	864:866	arg1	times					868:872	times	868:872	times	868:872	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	7	41	theme	peel	1280:1283	arg1	oil					1285:1287	emulsified pomelo peel oil	1262:1287	emulsified pomelo peel oil	1262:1287	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	5	42	from	effect	1065:1070	arg1	aureus					1078:1083	S. aureus	1075:1083	S. aureus	1075:1083	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	5	42	from	effect	1065:1070	arg1	coli					1092:1095	E. coli	1089:1095	E. coli	1089:1095	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	7	43	dep	suggests	1234:1241	arg1	increased					1497:1505	increased	1497:1505	could be increased	1488:1505	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	7	43	dep	suggests	1234:1241	arg1	using					1243:1247	using	1243:1247	using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative	1243:1359	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	3	44	theme	oil	580:582	arg1	composition					584:594	the essential oil composition	566:594	the essential oil composition	566:594	Analysis of the essential oil composition identified a total of 33 compounds with the main constituent, limonene accounting for 87.5% (940.07 mg/g) of the total.					
29642399	7	45	theme	food	1393:1396	arg1	processability					1375:1388	the processability	1371:1388	the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan,	1371:1486	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	1	46	theme	pomelo	208:213	arg1	oil					230:232	pomelo peel essential oil	208:232	pomelo peel essential oil	208:232	This study utilized pomelo steam distillation to isolate pomelo peel essential oil.					
29642399	0	47	theme	Pomelo	37:42	arg1	Oil					73:75	Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil	26:75	Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil	26:75	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	7	48	theme	pomelo	1467:1472	arg1	byproducts					1436:1445	the byproducts	1432:1445	the byproducts of the Taiwan Matou pomelo and chitosan	1432:1485	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	2	49	theme	acetylation	485:495	arg1	degree					475:480	degree	475:480	degree of acetylation	475:495	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	5	50	theme	0.03	993:996	arg1	%					997:997	%	997:997	%	997:997	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	2	51	theme	water-soluble	451:463	arg1	chitosan					465:472	water-soluble chitosan	451:472	water-soluble chitosan (degree of acetylation, DA = 54.8%)	451:508	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	1	52	theme	peel	215:218	arg1	oil					230:232	pomelo peel essential oil	208:232	pomelo peel essential oil	208:232	This study utilized pomelo steam distillation to isolate pomelo peel essential oil.					
29642399	0	53	theme	grandis	52:58	arg1	Osbeck					60:65	Citrus grandis Osbeck	45:65	Citrus grandis Osbeck	45:65	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	0	53	theme	grandis	52:58	arg1	Pomelo					37:42	Emulsified Pomelo	26:42	Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil	26:75	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	4	54	dep	times	868:872	arg1	lower					874:878	lower	874:878	lower	874:878	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	2	55	dep	=	501:501	arg1	degree					475:480	degree	475:480	degree of acetylation	475:495	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	3	56	theme	compounds	621:629	arg1	total					609:613	a total	607:613	a total of 33 compounds	607:629	Analysis of the essential oil composition identified a total of 33 compounds with the main constituent, limonene accounting for 87.5% (940.07 mg/g) of the total.					
29642399	5	57	theme	antibacterial	1051:1063	arg1	effect					1065:1070	the strongest synergetic antibacterial effect	1026:1070	the strongest synergetic antibacterial effect on S. aureus and E. coli	1026:1095	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	0	58	theme	Citrus	45:50	arg1	Osbeck					60:65	Citrus grandis Osbeck	45:65	Citrus grandis Osbeck	45:65	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	0	58	theme	Citrus	45:50	arg1	Pomelo					37:42	Emulsified Pomelo	26:42	Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil	26:75	Antibacterial Activity of Emulsified Pomelo (Citrus grandis Osbeck) Peel Oil and Water-Soluble Chitosan on Staphylococcus aureus and Escherichia coli.					
29642399	7	59	theme	Taiwan	1454:1459	arg1	pomelo					1467:1472	the Taiwan Matou pomelo	1450:1472	the Taiwan Matou pomelo	1450:1472	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	5	60	theme	0.4	961:963	arg1	%					964:964	%	964:964	%	964:964	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	3	61	theme	essential	570:578	arg1	composition					584:594	the essential oil composition	566:594	the essential oil composition	566:594	Analysis of the essential oil composition identified a total of 33 compounds with the main constituent, limonene accounting for 87.5% (940.07 mg/g) of the total.					
29642399	2	62	theme	antibacterial	333:345	arg1	activity					347:354	the antibacterial activity	329:354	the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli	329:538	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	7	63	theme	Matou	1461:1465	arg1	pomelo					1467:1472	the Taiwan Matou pomelo	1450:1472	the Taiwan Matou pomelo	1450:1472	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	5	64	theme	%	964:964	arg1	emulsion					980:987	0.4% essential oil emulsion	961:987	0.4% essential oil emulsion	961:987	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	3	65	theme	composition	584:594	arg1	Analysis					554:561	Analysis	554:561	Analysis of the essential oil composition	554:594	Analysis of the essential oil composition identified a total of 33 compounds with the main constituent, limonene accounting for 87.5% (940.07 mg/g) of the total.					
29642399	1	66	theme	essential	220:228	arg1	oil					230:232	pomelo peel essential oil	208:232	pomelo peel essential oil	208:232	This study utilized pomelo steam distillation to isolate pomelo peel essential oil.					
29642399	4	67	theme	peel	727:730	arg1	oil					732:734	The pomelo peel oil	716:734	The pomelo peel oil	716:734	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	3	68	theme	main	640:643	arg1	constituent					645:655	the main constituent	636:655	the main constituent	636:655	Analysis of the essential oil composition identified a total of 33 compounds with the main constituent, limonene accounting for 87.5% (940.07 mg/g) of the total.					
29642399	3	68	theme	main	640:643	arg1	limonene					658:665	limonene	658:665	limonene accounting for 87.5% (940.07 mg/g) of the total	658:713	Analysis of the essential oil composition identified a total of 33 compounds with the main constituent, limonene accounting for 87.5% (940.07 mg/g) of the total.					
29642399	2	69	from	conditions	418:427	arg1	emulsions					377:385	the essential oil emulsions	359:385	the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%)	359:508	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	69	from	conditions	418:427	arg1	activity					347:354	the antibacterial activity	329:354	the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli	329:538	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	5	70	theme	essential	966:974	arg1	emulsion					980:987	0.4% essential oil emulsion	961:987	0.4% essential oil emulsion	961:987	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	4	71	theme	essential	897:905	arg1	oil					907:909	the essential oil	893:909	the essential oil without homogenization	893:932	The pomelo peel oil was emulsified through homogenization at 24,000 rpm, resulting in a minimal inhibitory concentration (MIC) for E. coli that was 1.9 times lower than that of the essential oil without homogenization.					
29642399	2	72	theme	speed	412:416	arg1	conditions					418:427	different homogenizer speed conditions and concentrations	390:446	conditions	418:427	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	7	73	theme	economic	1414:1421	arg1	value					1423:1427	the economic value	1410:1427	the economic value of the byproducts of the Taiwan Matou pomelo and chitosan	1410:1485	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	2	74	theme	oil	373:375	arg1	emulsions					377:385	the essential oil emulsions	359:385	the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%)	359:508	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	75	theme	different	390:398	arg1	conditions					418:427	different homogenizer speed conditions and concentrations	390:446	conditions	418:427	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	5	76	theme	strongest	1030:1038	arg1	effect					1065:1070	the strongest synergetic antibacterial effect	1026:1070	the strongest synergetic antibacterial effect on S. aureus and E. coli	1026:1095	In addition, a mixture of 0.4% essential oil emulsion and 0.03% water-soluble chitosan had the strongest synergetic antibacterial effect on S. aureus and E. coli at pH 7.4.					
29642399	1	77	used	utilized	162:169	arg2	study					156:160	This study	151:160	This study	151:160	This study utilized pomelo steam distillation to isolate pomelo peel essential oil.					
29642399	2	78	from	concentrations	433:446	arg1	emulsions					377:385	the essential oil emulsions	359:385	the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%)	359:508	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	78	from	concentrations	433:446	arg1	activity					347:354	the antibacterial activity	329:354	the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli	329:538	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	2	79	theme	essential	363:371	arg1	emulsions					377:385	the essential oil emulsions	359:385	the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%)	359:508	The constituents were then analyzed through gas chromatography-mass spectrometry (GC-MS), and the antibacterial activity of the essential oil emulsions at different homogenizer speed conditions and concentrations of water-soluble chitosan (degree of acetylation, DA = 54.8%) against S. aureus and E. coli was examined.					
29642399	7	80	theme	byproducts	1436:1445	arg1	value					1423:1427	the economic value	1410:1427	the economic value of the byproducts of the Taiwan Matou pomelo and chitosan	1410:1485	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
29642399	7	80	theme	byproducts	1436:1445	arg1	food					1393:1396	food	1393:1396	food	1393:1396	Hence, this study suggests using a mixture of emulsified pomelo peel oil and water-soluble chitosan to develop a novel natural food preservative, and that the processability of food, as well as the economic value of the byproducts of the Taiwan Matou pomelo and chitosan, could be increased.					
30209873	0	0	theme	dental	96:101	arg1	abutments					103:111	alumina dental abutments	88:111	alumina dental abutments	88:111	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	9	1	theme	human	1314:1318	arg1	cells					1340:1344	The human gingival fibroblast cells	1310:1344	The human gingival fibroblast cells	1310:1344	The human gingival fibroblast cells were used to evaluate cytocompatibility by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay.					
30209873	7	2	theme	scanning	1119:1126	arg1	microscopy					1137:1146	scanning electron microscopy	1119:1146	scanning electron microscopy	1119:1146	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	11	3	theme	fibroblast	1673:1682	arg1	cells					1684:1688	human gingival fibroblast cells	1658:1688	human gingival fibroblast cells	1658:1688	The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces.					
30209873	0	4	theme	alumina	88:94	arg1	abutments					103:111	alumina dental abutments	88:111	alumina dental abutments	88:111	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	6	5	theme	thermocycle	899:909	arg1	apparatus					911:919	a thermocycle apparatus	897:919	a thermocycle apparatus	897:919	Additionally, an aging process with a thermocycle apparatus was applied on the coated materials to mimic the oral environment.					
30209873	7	6	theme	electron	1128:1135	arg1	microscopy					1137:1146	scanning electron microscopy	1119:1146	scanning electron microscopy	1119:1146	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	12	7	theme	efficient	1824:1832	arg1	CONCLUSION					1755:1764	CONCLUSION	1755:1764	CONCLUSION Coating zirconia and alumina surfaces with chitosan	1755:1816	CONCLUSION Coating zirconia and alumina surfaces with chitosan is an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro.					
30209873	12	7	theme	efficient	1824:1832	arg1	modification					1842:1853	an efficient surface modification	1821:1853	an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro	1821:1929	CONCLUSION Coating zirconia and alumina surfaces with chitosan is an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro.					
30209873	4	8	theme	research	653:660	arg1	aim					641:643	The main aim	632:643	The main aim of this research	632:660	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	9	9	theme	fibroblast	1329:1338	arg1	cells					1340:1344	The human gingival fibroblast cells	1310:1344	The human gingival fibroblast cells	1310:1344	The human gingival fibroblast cells were used to evaluate cytocompatibility by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay.					
30209873	7	10	theme	samples	1110:1116	arg1	surfaces					1045:1052	the surfaces	1041:1052	the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples	1041:1116	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	4	11	theme	alumina	749:755	arg1	biocompatibility					713:728	biocompatibility	713:728	biocompatibility	713:728	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	4	11	theme	alumina	749:755	arg1	characteristics					693:707	surface characteristics	685:707	surface characteristics	685:707	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	3	12	from	smooth	512:517	arg1	region					561:566	the transmucosal region	544:566	the transmucosal region	544:566	However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.					
30209873	0	13	theme	abutments	103:111	arg1	biocompatibility					39:54	biocompatibility	39:54	biocompatibility	39:54	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	0	13	theme	abutments	103:111	arg1	structure					25:33	surface structure	17:33	surface structure	17:33	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	11	14	dep	attachment	1626:1635	arg1	The					1622:1624	The	1622:1624	The	1622:1624	The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces.					
30209873	9	15	used	used	1351:1354	arg2	cells					1340:1344	The human gingival fibroblast cells	1310:1344	The human gingival fibroblast cells	1310:1344	The human gingival fibroblast cells were used to evaluate cytocompatibility by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay.					
30209873	4	16	theme	chitosan-coated	733:747	arg1	alumina					749:755	chitosan-coated alumina	733:755	chitosan-coated alumina	733:755	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	1	17	theme	bone-implant	265:276	arg1	interface					278:286	the bone-implant interface	261:286	the bone-implant interface	261:286	BACKGROUND For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms.					
30209873	5	18	dep	MATERIALS	780:788	arg1	substrates					806:815	The substrates	802:815	The substrates	802:815	MATERIALS AND METHODS The substrates were coated via solution casting technique.					
30209873	8	19	theme	X-ray	1282:1286	arg1	analysis					1300:1307	X-ray diffraction analysis	1282:1307	X-ray diffraction analysis	1282:1307	The phases and bonds characterized by Fourier transform infrared spectroscopy and X-ray diffraction analysis.					
30209873	4	20	theme	zirconia	761:768	arg1	surfaces					770:777	zirconia surfaces	761:777	zirconia surfaces	761:777	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	6	21	theme	oral	970:973	arg1	environment					975:985	the oral environment	966:985	the oral environment	966:985	Additionally, an aging process with a thermocycle apparatus was applied on the coated materials to mimic the oral environment.					
30209873	6	22	theme	aging	878:882	arg1	process					884:890	an aging process	875:890	an aging process with a thermocycle apparatus	875:919	Additionally, an aging process with a thermocycle apparatus was applied on the coated materials to mimic the oral environment.					
30209873	7	23	theme	energy	1152:1157	arg1	spectrometry					1176:1187	energy dispersive X-ray spectrometry	1152:1187	energy dispersive X-ray spectrometry	1152:1187	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	8	24	dep	transform	1246:1254	arg1	infrared					1256:1263	infrared	1256:1263	transform infrared spectroscopy and X-ray diffraction analysis	1246:1307	The phases and bonds characterized by Fourier transform infrared spectroscopy and X-ray diffraction analysis.					
30209873	9	25	theme	salt	1445:1448	arg1	assay					1450:1454	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay	1389:1454	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay	1389:1454	The human gingival fibroblast cells were used to evaluate cytocompatibility by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay.					
30209873	7	26	dep	morphology	1002:1011	arg1	the					998:1000	the	998:1000	the	998:1000	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	7	27	theme	chitosan-coated	1068:1082	arg1	samples					1110:1116	untreated, chitosan-coated, and chitosan-coated-aged samples	1057:1116	untreated, chitosan-coated, and chitosan-coated-aged samples	1057:1116	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	2	28	theme	implant	390:396	arg1	market					398:403	the dental implant market	379:403	the dental implant market	379:403	Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems are widely used in the area.					
30209873	2	29	theme	zirconia	428:435	arg1	systems					453:459	alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems	406:459	alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems	406:459	Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems are widely used in the area.					
30209873	2	30	theme	dental	383:388	arg1	market					398:403	the dental implant market	379:403	the dental implant market	379:403	Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems are widely used in the area.					
30209873	0	31	theme	structure	25:33	arg1	Investigation					0:12	Investigation	0:12	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.	0:112	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	7	32	theme	untreated	1057:1065	arg1	samples					1110:1116	untreated, chitosan-coated, and chitosan-coated-aged samples	1057:1116	untreated, chitosan-coated, and chitosan-coated-aged samples	1057:1116	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	12	33	theme	surface	1834:1840	arg1	CONCLUSION					1755:1764	CONCLUSION	1755:1764	CONCLUSION Coating zirconia and alumina surfaces with chitosan	1755:1816	CONCLUSION Coating zirconia and alumina surfaces with chitosan is an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro.					
30209873	12	33	theme	surface	1834:1840	arg1	modification					1842:1853	an efficient surface modification	1821:1853	an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro	1821:1929	CONCLUSION Coating zirconia and alumina surfaces with chitosan is an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro.					
30209873	7	34	theme	chitosan-coated-aged	1089:1108	arg1	samples					1110:1116	untreated, chitosan-coated, and chitosan-coated-aged samples	1057:1116	untreated, chitosan-coated, and chitosan-coated-aged samples	1057:1116	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	8	35	theme	diffraction	1288:1298	arg1	analysis					1300:1307	X-ray diffraction analysis	1282:1307	X-ray diffraction analysis	1282:1307	The phases and bonds characterized by Fourier transform infrared spectroscopy and X-ray diffraction analysis.					
30209873	0	36	theme	surface	17:23	arg1	structure					25:33	surface structure	17:33	surface structure	17:33	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	7	37	theme	surfaces	1045:1052	arg1	morphology					1002:1011	morphology	1002:1011	morphology	1002:1011	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	7	37	theme	surfaces	1045:1052	arg1	composition					1026:1036	chemical composition	1017:1036	chemical composition	1017:1036	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	10	38	theme	aging	1549:1553	arg1	process					1555:1561	the aging process	1545:1561	the aging process	1545:1561	RESULTS It was observed that both substrates were successfully coated with chitosan and the aging process did not significantly affect the integrity of the coating.					
30209873	4	39	dep	characteristics	693:707	arg1	the					681:683	the	681:683	the	681:683	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	3	40	from	region	561:566	arg1	smooth					512:517	smooth	512:517	smooth	512:517	However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.					
30209873	2	41	theme	Al2	415:417	arg1	alumina					406:412	alumina	406:412	alumina (Al2 O3 )	406:422	Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems are widely used in the area.					
30209873	2	41	theme	Al2	415:417	arg1	O3					419:420	Al2 O3	415:420	Al2 O3	415:420	Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems are widely used in the area.					
30209873	0	42	theme	biocompatibility	39:54	arg1	Investigation					0:12	Investigation	0:12	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.	0:112	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	7	43	used	used	1194:1197	arg2	microscopy					1137:1146	scanning electron microscopy	1119:1146	scanning electron microscopy	1119:1146	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	7	43	used	used	1194:1197	arg2	spectrometry					1176:1187	energy dispersive X-ray spectrometry	1152:1187	energy dispersive X-ray spectrometry	1152:1187	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	6	44	with	process	884:890	arg1	apparatus					911:919	a thermocycle apparatus	897:919	a thermocycle apparatus	897:919	Additionally, an aging process with a thermocycle apparatus was applied on the coated materials to mimic the oral environment.					
30209873	4	45	theme	main	636:639	arg1	aim					641:643	The main aim	632:643	The main aim of this research	632:660	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	5	46	theme	solution	833:840	arg1	technique					850:858	solution casting technique	833:858	solution casting technique	833:858	MATERIALS AND METHODS The substrates were coated via solution casting technique.					
30209873	2	47	theme	implant	445:451	arg1	systems					453:459	alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems	406:459	alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems	406:459	Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems are widely used in the area.					
30209873	6	48	theme	coated	940:945	arg1	materials					947:955	the coated materials	936:955	the coated materials	936:955	Additionally, an aging process with a thermocycle apparatus was applied on the coated materials to mimic the oral environment.					
30209873	7	49	theme	dispersive	1159:1168	arg1	spectrometry					1176:1187	energy dispersive X-ray spectrometry	1152:1187	energy dispersive X-ray spectrometry	1152:1187	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	11	50	theme	chitosan-coated	1729:1743	arg1	surfaces					1745:1752	chitosan-coated surfaces	1729:1752	chitosan-coated surfaces	1729:1752	The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces.					
30209873	7	51	theme	X-ray	1170:1174	arg1	spectrometry					1176:1187	energy dispersive X-ray spectrometry	1152:1187	energy dispersive X-ray spectrometry	1152:1187	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	5	52	theme	casting	842:848	arg1	technique					850:858	solution casting technique	833:858	solution casting technique	833:858	MATERIALS AND METHODS The substrates were coated via solution casting technique.					
30209873	1	53	theme	surrounding	213:223	arg1	barrier					237:243	the surrounding soft tissue barrier	209:243	the surrounding soft tissue barrier	209:243	BACKGROUND For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms.					
30209873	0	54	theme	chitosan-coated	59:73	arg1	zirconia					75:82	chitosan-coated zirconia	59:82	chitosan-coated zirconia	59:82	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	3	55	theme	surrounding	601:611	arg1	tissues					613:619	the surrounding tissues	597:619	the surrounding tissues	597:619	However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.					
30209873	11	56	theme	human	1658:1662	arg1	cells					1684:1688	human gingival fibroblast cells	1658:1688	human gingival fibroblast cells	1658:1688	The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces.					
30209873	1	57	theme	long-term	129:137	arg1	success					139:145	long-term success	129:145	long-term success of dental implants	129:164	BACKGROUND For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms.					
30209873	3	58	dep	smooth	512:517	arg1	surfaces					532:539	surfaces	532:539	surfaces	532:539	However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.					
30209873	9	59	theme	4,5-dimethylthiazol-2-yl	1394:1417	arg1	assay					1450:1454	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay	1389:1454	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay	1389:1454	The human gingival fibroblast cells were used to evaluate cytocompatibility by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay.					
30209873	8	60	dep	Fourier	1238:1244	arg1	transform					1246:1254	transform	1246:1254	transform infrared spectroscopy and X-ray diffraction analysis	1246:1307	The phases and bonds characterized by Fourier transform infrared spectroscopy and X-ray diffraction analysis.					
30209873	3	61	theme	transmucosal	548:559	arg1	region					561:566	the transmucosal region	544:566	the transmucosal region	544:566	However, they provide smooth and bioinert surfaces in the transmucosal region, which poorly integrate with the surrounding tissues.					
30209873	12	62	theme	materials	1912:1920	arg1	biocompatibility					1870:1885	biocompatibility	1870:1885	biocompatibility	1870:1885	CONCLUSION Coating zirconia and alumina surfaces with chitosan is an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro.					
30209873	12	62	theme	materials	1912:1920	arg1	bioactivity					1891:1901	bioactivity	1891:1901	bioactivity	1891:1901	CONCLUSION Coating zirconia and alumina surfaces with chitosan is an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro.					
30209873	10	63	theme	coating	1613:1619	arg1	integrity					1596:1604	the integrity	1592:1604	the integrity of the coating	1592:1619	RESULTS It was observed that both substrates were successfully coated with chitosan and the aging process did not significantly affect the integrity of the coating.					
30209873	7	64	theme	chemical	1017:1024	arg1	composition					1026:1036	chemical composition	1017:1036	chemical composition	1017:1036	To define the morphology and chemical composition of the surfaces of untreated, chitosan-coated, and chitosan-coated-aged samples, scanning electron microscopy and energy dispersive X-ray spectrometry were used.					
30209873	1	65	theme	implants	157:164	arg1	success					139:145	long-term success	129:145	long-term success of dental implants	129:164	BACKGROUND For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms.					
30209873	4	66	theme	surface	685:691	arg1	characteristics					693:707	surface characteristics	685:707	surface characteristics	685:707	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	10	67	dep	RESULTS	1457:1463	arg1	observed					1472:1479	observed	1472:1479	was observed that both substrates were successfully coated with chitosan and the aging process did not significantly affect the integrity of the coating	1468:1619	RESULTS It was observed that both substrates were successfully coated with chitosan and the aging process did not significantly affect the integrity of the coating.					
30209873	1	68	theme	soft	225:228	arg1	barrier					237:243	the surrounding soft tissue barrier	209:243	the surrounding soft tissue barrier	209:243	BACKGROUND For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms.					
30209873	2	69	used	used	472:475	arg2	systems					453:459	alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems	406:459	alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems	406:459	Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems are widely used in the area.					
30209873	4	70	theme	surfaces	770:777	arg1	biocompatibility					713:728	biocompatibility	713:728	biocompatibility	713:728	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	4	70	theme	surfaces	770:777	arg1	characteristics					693:707	surface characteristics	685:707	surface characteristics	685:707	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	11	71	theme	cells	1684:1688	arg1	proliferation					1641:1653	proliferation	1641:1653	proliferation	1641:1653	The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces.					
30209873	11	71	theme	cells	1684:1688	arg1	attachment					1626:1635	attachment	1626:1635	attachment	1626:1635	The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces.					
30209873	1	72	theme	dental	150:155	arg1	implants					157:164	dental implants	150:164	dental implants	150:164	BACKGROUND For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms.					
30209873	12	73	theme	alumina	1787:1793	arg1	surfaces					1795:1802	alumina surfaces	1787:1802	alumina surfaces	1787:1802	CONCLUSION Coating zirconia and alumina surfaces with chitosan is an efficient surface modification for increasing biocompatibility and bioactivity of these materials in vitro.					
30209873	1	74	theme	tissue	230:235	arg1	barrier					237:243	the surrounding soft tissue barrier	209:243	the surrounding soft tissue barrier	209:243	BACKGROUND For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms.					
30209873	2	75	theme	alumina	406:412	arg1	systems					453:459	alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems	406:459	alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems	406:459	Although implants based on titanium and its alloys still dominate the dental implant market, alumina (Al2 O3 ) and zirconia (ZrO2 ) implant systems are widely used in the area.					
30209873	1	76	dep	BACKGROUND	114:123	arg1	essential					173:181	essential	173:181	essential	173:181	BACKGROUND For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms.					
30209873	1	77	theme	barrier	237:243	arg1	health					199:204	the health	195:204	the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms	195:310	BACKGROUND For long-term success of dental implants, it is essential to maintain the health of the surrounding soft tissue barrier, which protects the bone-implant interface from the microorganisms.					
30209873	0	78	theme	zirconia	75:82	arg1	biocompatibility					39:54	biocompatibility	39:54	biocompatibility	39:54	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	0	78	theme	zirconia	75:82	arg1	structure					25:33	surface structure	17:33	surface structure	17:33	Investigation of surface structure and biocompatibility of chitosan-coated zirconia and alumina dental abutments.					
30209873	9	79	theme	gingival	1320:1327	arg1	cells					1340:1344	The human gingival fibroblast cells	1310:1344	The human gingival fibroblast cells	1310:1344	The human gingival fibroblast cells were used to evaluate cytocompatibility by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay.					
30209873	4	80	dep	OBJECTIVE	622:630	arg1	was					662:664	was	662:664	was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces	662:777	OBJECTIVE The main aim of this research was to investigate the surface characteristics and biocompatibility of chitosan-coated alumina and zirconia surfaces.					
30209873	11	81	theme	gingival	1664:1671	arg1	cells					1684:1688	human gingival fibroblast cells	1658:1688	human gingival fibroblast cells	1658:1688	The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces.					
30209873	9	82	theme	-2,5-diphenyl-tetrazolium	1419:1443	arg1	assay					1450:1454	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay	1389:1454	a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay	1389:1454	The human gingival fibroblast cells were used to evaluate cytocompatibility by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium salt assay.					
30209873	11	83	theme	surfaces	1745:1752	arg1	kinds					1720:1724	both kinds	1715:1724	both kinds of chitosan-coated surfaces	1715:1752	The attachment and proliferation of human gingival fibroblast cells were shown to be good on both kinds of chitosan-coated surfaces.					
30980948	9	0	theme	research	1886:1893	arg1	groups					1895:1900	three independent research groups	1868:1900	three independent research groups using distinct poly(I:C) administration protocols in mice	1868:1958	This batch-to-batch variability was confirmed by three independent research groups using distinct poly(I:C) administration protocols in mice.					
30980948	1	1	theme	immune-mediated	348:362	arg1	disorders					383:391	immune-mediated neurodevelopmental disorders	348:391	immune-mediated neurodevelopmental disorders	348:391	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	10	2	theme	different	2007:2015	arg1	products					2027:2034	different poly(I:C) products	2007:2034	different poly(I:C) products	2007:2034	Taken together, the present data confirm that different poly(I:C) products can induce varying immune responses and can differentially affect maternal physiology and pregnancy outcomes.					
30980948	8	3	from	batches	1722:1728	arg1	terms					1765:1769	terms	1765:1769	terms of their effects on spontaneous abortion rates	1765:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	9	4	from	protocols	1942:1950	arg1	mice					1955:1958	mice	1955:1958	mice	1955:1958	This batch-to-batch variability was confirmed by three independent research groups using distinct poly(I:C) administration protocols in mice.					
30980948	5	5	theme	extensive	1269:1277	arg1	effects					1279:1285	more extensive effects	1264:1285	more extensive effects on thermoregulation	1264:1305	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	1	6	theme	viral	223:227	arg1	administration					201:214	administration	201:214	administration of the viral mimetic	201:235	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	4	7	theme	high	972:975	arg1	nucleotides					1011:1021	1000-6000 nucleotides long	1001:1026	1000-6000 nucleotides long	1001:1026	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	7	theme	high	972:975	arg1	range					994:998	the high molecular weight range	968:998	the high molecular weight range (1000-6000 nucleotides long)	968:1027	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	9	8	theme	poly	1917:1920	arg1	protocols					1942:1950	distinct poly(I:C) administration protocols	1908:1950	distinct poly(I:C) administration protocols in mice	1908:1958	This batch-to-batch variability was confirmed by three independent research groups using distinct poly(I:C) administration protocols in mice.					
30980948	1	9	theme	mental	397:402	arg1	illnesses					404:412	mental illnesses	397:412	mental illnesses	397:412	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	10	theme	Maternal	143:150	arg1	poly					238:241	poly	238:241	poly(I:C)	238:246	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	10	theme	Maternal	143:150	arg1	tools					281:285	experimental tools	268:285	experimental tools	268:285	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	10	theme	Maternal	143:150	arg1	models					176:181	Maternal immune activation (MIA) models	143:181	Maternal immune activation (MIA) models that are based on administration of the viral mimetic	143:235	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	2	11	theme	immunogenicity	538:551	arg1	terms					523:527	terms	523:527	terms of their immunogenicity	523:551	Evidence from investigations in non-pregnant rodents suggests that different poly(I:C) products can vary in terms of their immunogenicity, even if they are obtained from the same vendor.					
30980948	4	12	theme	weight	987:992	arg1	nucleotides					1011:1021	1000-6000 nucleotides long	1001:1026	1000-6000 nucleotides long	1001:1026	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	12	theme	weight	987:992	arg1	range					994:998	the high molecular weight range	968:998	the high molecular weight range (1000-6000 nucleotides long)	968:1027	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	3	13	theme	pregnant	657:664	arg1	mice					666:669	pregnant mice	657:669	pregnant mice	657:669	The present study aimed at extending these findings to pregnant mice, while also controlling various poly(I:C) products for potential contamination with lipopolysaccharide (LPS).					
30980948	5	14	theme	weight	1239:1244	arg1	fragments					1246:1254	high molecular weight fragments	1224:1254	high molecular weight fragments	1224:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	11	15	theme	molecular	2262:2270	arg1	composition					2272:2282	precise molecular composition	2254:2282	precise molecular composition	2254:2282	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	1	16	theme	activation	159:168	arg1	poly					238:241	poly	238:241	poly(I:C)	238:246	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	16	theme	activation	159:168	arg1	tools					281:285	experimental tools	268:285	experimental tools	268:285	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	16	theme	activation	159:168	arg1	models					176:181	Maternal immune activation (MIA) models	143:181	Maternal immune activation (MIA) models that are based on administration of the viral mimetic	143:235	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	10	17	theme	varying	2047:2053	arg1	responses					2062:2070	varying immune responses	2047:2070	varying immune responses	2047:2070	Taken together, the present data confirm that different poly(I:C) products can induce varying immune responses and can differentially affect maternal physiology and pregnancy outcomes.					
30980948	4	18	theme	dsRNA	949:953	arg1	fragments					955:963	dsRNA fragments	949:963	dsRNA fragments	949:963	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	12	19	theme	models	2538:2543	arg1	design					2473:2478	design	2473:2478	design	2473:2478	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	12	19	theme	models	2538:2543	arg1	interpretation					2500:2513	interpretation	2500:2513	interpretation	2500:2513	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	12	19	theme	models	2538:2543	arg1	implementation					2481:2494	implementation	2481:2494	implementation	2481:2494	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	0	20	from	thermoregulation	38:53	arg1	models					105:110	mouse models	99:110	mouse models of maternal immune activation	99:140	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	9	21	theme	administration	1927:1940	arg1	protocols					1942:1950	distinct poly(I:C) administration protocols	1908:1950	distinct poly(I:C) administration protocols in mice	1908:1958	This batch-to-batch variability was confirmed by three independent research groups using distinct poly(I:C) administration protocols in mice.					
30980948	0	22	theme	mouse	99:103	arg1	models					105:110	mouse models	99:110	mouse models of maternal immune activation	99:140	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	12	23	theme	phenotype	2393:2401	arg1	data					2403:2406	phenotype data	2393:2406	phenotype data	2393:2406	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	6	24	theme	dsRNA	1482:1486	arg1	weight					1475:1480	high molecular weight dsRNA	1460:1486	high molecular weight dsRNA (HMW)	1460:1492	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	24	theme	dsRNA	1482:1486	arg1	HMW					1489:1491	HMW	1489:1491	HMW	1489:1491	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	8	25	theme	different	1712:1720	arg1	batches					1722:1728	different batches	1712:1728	different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates	1712:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	11	26	theme	product	2310:2316	arg1	immunogenicity					2288:2301	immunogenicity	2288:2301	immunogenicity	2288:2301	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	11	26	theme	product	2310:2316	arg1	composition					2272:2282	precise molecular composition	2254:2282	precise molecular composition	2254:2282	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	4	27	from	amount	939:944	arg1	nucleotides					1011:1021	1000-6000 nucleotides long	1001:1026	1000-6000 nucleotides long	1001:1026	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	27	from	amount	939:944	arg1	range					994:998	the high molecular weight range	968:998	the high molecular weight range (1000-6000 nucleotides long)	968:1027	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	28	theme	significant	790:800	arg1	variability					802:812	significant variability	790:812	significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	790:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	12	29	theme	databases	2370:2378	arg1	establishment					2343:2355	the establishment	2339:2355	the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models	2339:2543	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	0	30	theme	immune	124:129	arg1	activation					131:140	maternal immune activation	115:140	maternal immune activation	115:140	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	2	31	theme	non-pregnant	447:458	arg1	rodents					460:466	non-pregnant rodents	447:466	non-pregnant rodents	447:466	Evidence from investigations in non-pregnant rodents suggests that different poly(I:C) products can vary in terms of their immunogenicity, even if they are obtained from the same vendor.					
30980948	11	32	theme	precise	2254:2260	arg1	composition					2272:2282	precise molecular composition	2254:2282	precise molecular composition	2254:2282	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	3	33	theme	various	695:701	arg1	products					713:720	various poly(I:C) products	695:720	various poly(I:C) products	695:720	The present study aimed at extending these findings to pregnant mice, while also controlling various poly(I:C) products for potential contamination with lipopolysaccharide (LPS).					
30980948	0	34	theme	variability	23:33	arg1	Influence					0:8	Influence	0:8	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.	0:141	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	5	35	with	batches	1340:1346	arg1	amounts					1361:1367	minimal amounts	1353:1367	minimal amounts of high molecular weight fragments	1353:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	5	35	with	batches	1340:1346	arg1	fragments					1394:1402	high molecular weight fragments	1372:1402	high molecular weight fragments	1372:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	4	36	theme	potassium	853:861	arg1	salt					863:866	potassium salt	853:866	poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	843:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	6	37	theme	low	1560:1562	arg1	LMW					1582:1584	LMW	1582:1584	LMW	1582:1584	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	37	theme	low	1560:1562	arg1	weight					1574:1579	low molecular weight	1560:1579	low molecular weight (LMW) poly(I:C)	1560:1595	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	8	38	theme	I	1742:1742	arg1	poly					1737:1740	poly	1737:1740	the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates	1733:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	8	38	theme	I	1742:1742	arg1	C					1744:1744	I:C	1742:1744	I:C	1742:1744	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	4	39	with	amount	939:944	arg1	regards					1038:1044	regards	1038:1044	regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	1038:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	6	40	theme	molecular	1465:1473	arg1	weight					1475:1480	high molecular weight dsRNA	1460:1486	high molecular weight dsRNA (HMW)	1460:1492	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	40	theme	molecular	1465:1473	arg1	HMW					1489:1491	HMW	1489:1491	HMW	1489:1491	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	2	41	theme	different	482:490	arg1	products					502:509	different poly(I:C) products	482:509	different poly(I:C) products	482:509	Evidence from investigations in non-pregnant rodents suggests that different poly(I:C) products can vary in terms of their immunogenicity, even if they are obtained from the same vendor.					
30980948	0	42	from	Influence	0:8	arg1	thermoregulation					38:53	thermoregulation	38:53	thermoregulation	38:53	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	0	42	from	Influence	0:8	arg1	responses					63:71	immune responses	56:71	immune responses	56:71	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	0	42	from	Influence	0:8	arg1	outcomes					87:94	pregnancy outcomes	77:94	pregnancy outcomes	77:94	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	4	43	theme	maternal	1066:1073	arg1	thermoregulation					1075:1090	maternal thermoregulation	1066:1090	maternal thermoregulation	1066:1090	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	0	44	theme	immune	56:61	arg1	responses					63:71	immune responses	56:71	immune responses	56:71	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	6	45	dep	maternal	1508:1515	arg1	larger					1501:1506	larger	1501:1506	larger	1501:1506	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	11	46	dep	composition	2272:2282	arg1	the					2250:2252	the	2250:2252	the	2250:2252	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	10	47	theme	pregnancy	2126:2134	arg1	outcomes					2136:2143	pregnancy outcomes	2126:2143	pregnancy outcomes	2126:2143	Taken together, the present data confirm that different poly(I:C) products can induce varying immune responses and can differentially affect maternal physiology and pregnancy outcomes.					
30980948	6	48	theme	immune	1531:1536	arg1	responses					1538:1546	larger maternal and placental immune responses	1501:1546	larger maternal and placental immune responses	1501:1546	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	8	49	theme	potassium	1747:1755	arg1	salt					1757:1760	the poly(I:C) potassium salt	1733:1760	the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates	1733:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	3	50	with	contamination	736:748	arg1	LPS					775:777	LPS	775:777	LPS	775:777	The present study aimed at extending these findings to pregnant mice, while also controlling various poly(I:C) products for potential contamination with lipopolysaccharide (LPS).					
30980948	3	50	with	contamination	736:748	arg1	lipopolysaccharide					755:772	lipopolysaccharide	755:772	lipopolysaccharide (LPS)	755:778	The present study aimed at extending these findings to pregnant mice, while also controlling various poly(I:C) products for potential contamination with lipopolysaccharide (LPS).					
30980948	12	51	theme	I	2523:2523	arg1	poly					2518:2521	poly	2518:2521	poly(I:C)-based MIA models	2518:2543	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	12	51	theme	I	2523:2523	arg1	C					2525:2525	I:C	2523:2525	I:C	2523:2525	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	8	52	from	effects	1780:1786	arg1	rates					1812:1816	spontaneous abortion rates	1791:1816	spontaneous abortion rates	1791:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	2	53	theme	same	589:592	arg1	vendor					594:599	the same vendor	585:599	the same vendor	585:599	Evidence from investigations in non-pregnant rodents suggests that different poly(I:C) products can vary in terms of their immunogenicity, even if they are obtained from the same vendor.					
30980948	4	54	from	effects	1055:1061	arg1	placenta					1133:1140	placenta	1133:1140	placenta	1133:1140	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	54	from	effects	1055:1061	arg1	brain					1152:1156	fetal brain	1146:1156	fetal brain	1146:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	54	from	effects	1055:1061	arg1	plasma					1125:1130	maternal plasma	1116:1130	maternal plasma	1116:1130	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	54	from	effects	1055:1061	arg1	responses					1103:1111	immune responses	1096:1111	immune responses	1096:1111	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	54	from	effects	1055:1061	arg1	thermoregulation					1075:1090	maternal thermoregulation	1066:1090	maternal thermoregulation	1066:1090	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	6	55	theme	I	1592:1592	arg1	C					1594:1594	I:C	1592:1594	I:C	1592:1594	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	55	theme	I	1592:1592	arg1	poly					1587:1590	low molecular weight (LMW) poly	1560:1590	low molecular weight (LMW) poly(I:C)	1560:1595	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	5	56	theme	high	1372:1375	arg1	fragments					1394:1402	high molecular weight fragments	1372:1402	high molecular weight fragments	1372:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	11	57	dep	researchers	2175:2185	arg1	working					2187:2193	working	2187:2193	working	2187:2193	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	0	58	theme	poly	13:16	arg1	variability					23:33	poly(I:C) variability	13:33	poly(I:C) variability	13:33	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	4	59	from	vendor	891:896	arg1	terms					917:921	terms	917:921	terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	917:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	5	60	theme	weight	1387:1392	arg1	fragments					1394:1402	high molecular weight fragments	1372:1402	high molecular weight fragments	1372:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	0	61	theme	I	18:18	arg1	poly					13:16	poly	13:16	poly(I:C) variability	13:33	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	0	61	theme	I	18:18	arg1	C					20:20	I:C	18:20	I:C	18:20	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	1	62	theme	I	243:243	arg1	poly					238:241	poly	238:241	poly(I:C)	238:246	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	62	theme	I	243:243	arg1	C					245:245	I:C	243:245	I:C	243:245	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	8	63	theme	effects	1780:1786	arg1	terms					1765:1769	terms	1765:1769	terms of their effects on spontaneous abortion rates	1765:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	12	64	theme	poly	2518:2521	arg1	models					2538:2543	poly(I:C)-based MIA models	2518:2543	poly(I:C)-based MIA models	2518:2543	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	8	65	theme	poly	1737:1740	arg1	salt					1757:1760	the poly(I:C) potassium salt	1733:1760	the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates	1733:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	5	66	theme	larger	1206:1211	arg1	fragments					1246:1254	high molecular weight fragments	1224:1254	high molecular weight fragments	1224:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	5	66	theme	larger	1206:1211	arg1	amounts					1213:1219	larger amounts	1206:1219	larger amounts of high molecular weight fragments	1206:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	8	67	theme	spontaneous	1791:1801	arg1	rates					1812:1816	spontaneous abortion rates	1791:1816	spontaneous abortion rates	1791:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	11	68	from	immunogenicity	2288:2301	arg1	use					2321:2323	use	2321:2323	use	2321:2323	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	7	69	theme	variable	1604:1611	arg1	effects					1613:1619	These variable effects	1598:1619	These variable effects	1598:1619	These variable effects were unrelated to possible LPS contamination.					
30980948	4	70	theme	relative	930:937	arg1	amount					939:944	the relative amount	926:944	the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	926:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	70	theme	relative	930:937	arg1	fragments					955:963	dsRNA fragments	949:963	dsRNA fragments	949:963	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	6	71	theme	weight	1574:1579	arg1	C					1594:1594	I:C	1592:1594	I:C	1592:1594	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	71	theme	weight	1574:1579	arg1	poly					1587:1590	low molecular weight (LMW) poly	1560:1590	low molecular weight (LMW) poly(I:C)	1560:1595	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	5	72	theme	high	1224:1227	arg1	fragments					1246:1254	high molecular weight fragments	1224:1254	high molecular weight fragments	1224:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	7	73	theme	possible	1639:1646	arg1	contamination					1652:1664	possible LPS contamination	1639:1664	possible LPS contamination	1639:1664	These variable effects were unrelated to possible LPS contamination.					
30980948	4	74	from	terms	917:921	arg1	nucleotides					1011:1021	1000-6000 nucleotides long	1001:1026	1000-6000 nucleotides long	1001:1026	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	74	from	terms	917:921	arg1	range					994:998	the high molecular weight range	968:998	the high molecular weight range (1000-6000 nucleotides long)	968:1027	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	75	theme	maternal	1116:1123	arg1	plasma					1125:1130	maternal plasma	1116:1130	maternal plasma	1116:1130	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	5	76	from	effects	1279:1285	arg1	thermoregulation					1290:1305	thermoregulation	1290:1305	thermoregulation	1290:1305	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	0	77	from	outcomes	87:94	arg1	models					105:110	mouse models	99:110	mouse models of maternal immune activation	99:140	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	9	78	theme	independent	1874:1884	arg1	groups					1895:1900	three independent research groups	1868:1900	three independent research groups using distinct poly(I:C) administration protocols in mice	1868:1958	This batch-to-batch variability was confirmed by three independent research groups using distinct poly(I:C) administration protocols in mice.					
30980948	5	79	theme	salt	1190:1193	arg1	Batches					1159:1165	Batches	1159:1165	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments	1159:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	11	80	from	composition	2272:2282	arg1	use					2321:2323	use	2321:2323	use	2321:2323	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	10	81	theme	poly	2017:2020	arg1	products					2027:2034	different poly(I:C) products	2007:2034	different poly(I:C) products	2007:2034	Taken together, the present data confirm that different poly(I:C) products can induce varying immune responses and can differentially affect maternal physiology and pregnancy outcomes.					
30980948	9	82	theme	batch-to-batch	1824:1837	arg1	variability					1839:1849	This batch-to-batch variability	1819:1849	This batch-to-batch variability	1819:1849	This batch-to-batch variability was confirmed by three independent research groups using distinct poly(I:C) administration protocols in mice.					
30980948	12	83	theme	-based	2527:2532	arg1	models					2538:2543	poly(I:C)-based MIA models	2518:2543	poly(I:C)-based MIA models	2518:2543	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	1	84	theme	neurodevelopmental	364:381	arg1	disorders					383:391	immune-mediated neurodevelopmental disorders	348:391	immune-mediated neurodevelopmental disorders	348:391	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	4	85	theme	molecular	977:985	arg1	nucleotides					1011:1021	1000-6000 nucleotides long	1001:1026	1000-6000 nucleotides long	1001:1026	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	85	theme	molecular	977:985	arg1	range					994:998	the high molecular weight range	968:998	the high molecular weight range (1000-6000 nucleotides long)	968:1027	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	9	86	theme	distinct	1908:1915	arg1	protocols					1942:1950	distinct poly(I:C) administration protocols	1908:1950	distinct poly(I:C) administration protocols in mice	1908:1958	This batch-to-batch variability was confirmed by three independent research groups using distinct poly(I:C) administration protocols in mice.					
30980948	4	87	from	range	994:998	arg1	terms					917:921	terms	917:921	terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	917:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	12	88	dep	combine	2385:2391	arg1	aid					2465:2467	aid	2465:2467	can aid the design, implementation and interpretation of poly(I:C)-based MIA models	2461:2543	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	5	89	theme	molecular	1229:1237	arg1	fragments					1246:1254	high molecular weight fragments	1224:1254	high molecular weight fragments	1224:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	1	90	theme	immune	152:157	arg1	MIA					171:173	MIA	171:173	MIA	171:173	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	90	theme	immune	152:157	arg1	activation					159:168	immune activation	152:168	Maternal immune activation (MIA) models that are based on administration of the viral mimetic	143:235	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	11	91	theme	poly	2200:2203	arg1	models					2220:2225	poly(I:C)-based MIA models	2200:2225	poly(I:C)-based MIA models	2200:2225	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	10	92	theme	I	2022:2022	arg1	poly					2017:2020	poly	2017:2020	different poly(I:C) products	2007:2034	Taken together, the present data confirm that different poly(I:C) products can induce varying immune responses and can differentially affect maternal physiology and pregnancy outcomes.					
30980948	10	92	theme	I	2022:2022	arg1	C					2024:2024	I:C	2022:2024	I:C	2022:2024	Taken together, the present data confirm that different poly(I:C) products can induce varying immune responses and can differentially affect maternal physiology and pregnancy outcomes.					
30980948	4	93	theme	fragments	955:963	arg1	amount					939:944	the relative amount	926:944	the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	926:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	93	theme	fragments	955:963	arg1	fragments					955:963	dsRNA fragments	949:963	dsRNA fragments	949:963	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	94	dep	I	848:848	arg1	C					850:850	C	850:850	C	850:850	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	5	95	theme	fragments	1246:1254	arg1	fragments					1246:1254	high molecular weight fragments	1224:1254	high molecular weight fragments	1224:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	5	95	theme	fragments	1246:1254	arg1	amounts					1213:1219	larger amounts	1206:1219	larger amounts of high molecular weight fragments	1206:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	11	96	dep	I	2205:2205	arg1	C					2207:2207	C	2207:2207	C	2207:2207	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	2	97	theme	I	497:497	arg1	poly					492:495	poly	492:495	different poly(I:C) products	482:509	Evidence from investigations in non-pregnant rodents suggests that different poly(I:C) products can vary in terms of their immunogenicity, even if they are obtained from the same vendor.					
30980948	2	97	theme	I	497:497	arg1	C					499:499	I:C	497:499	I:C	497:499	Evidence from investigations in non-pregnant rodents suggests that different poly(I:C) products can vary in terms of their immunogenicity, even if they are obtained from the same vendor.					
30980948	2	98	from	investigations	429:442	arg1	rodents					460:466	non-pregnant rodents	447:466	non-pregnant rodents	447:466	Evidence from investigations in non-pregnant rodents suggests that different poly(I:C) products can vary in terms of their immunogenicity, even if they are obtained from the same vendor.					
30980948	2	98	from	investigations	429:442	arg1	Evidence					415:422	Evidence	415:422	Evidence from investigations in non-pregnant rodents	415:466	Evidence from investigations in non-pregnant rodents suggests that different poly(I:C) products can vary in terms of their immunogenicity, even if they are obtained from the same vendor.					
30980948	9	99	theme	I	1922:1922	arg1	C					1924:1924	I:C	1922:1924	I:C	1922:1924	This batch-to-batch variability was confirmed by three independent research groups using distinct poly(I:C) administration protocols in mice.					
30980948	9	99	theme	I	1922:1922	arg1	poly					1917:1920	poly	1917:1920	distinct poly(I:C) administration protocols in mice	1908:1958	This batch-to-batch variability was confirmed by three independent research groups using distinct poly(I:C) administration protocols in mice.					
30980948	4	100	dep	poly	843:846	arg1	salt					863:866	potassium salt	853:866	poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	843:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	1	101	theme	neuronal	296:303	arg1	dysfunctions					320:331	neuronal and behavioral dysfunctions	296:331	neuronal and behavioral dysfunctions	296:331	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	102	used	used	260:263	arg2	models					176:181	Maternal immune activation (MIA) models	143:181	Maternal immune activation (MIA) models that are based on administration of the viral mimetic	143:235	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	102	used	used	260:263	arg2	tools					281:285	experimental tools	268:285	experimental tools	268:285	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	102	used	used	260:263	arg2	poly					238:241	poly	238:241	poly(I:C)	238:246	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	4	103	theme	different	822:830	arg1	batches					832:838	different batches	822:838	different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	822:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	12	104	theme	reference	2360:2368	arg1	databases					2370:2378	reference databases	2360:2378	reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models	2360:2543	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	4	105	theme	poly	843:846	arg1	batches					832:838	different batches	822:838	different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	822:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	0	106	theme	maternal	115:122	arg1	activation					131:140	maternal immune activation	115:140	maternal immune activation	115:140	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	1	107	theme	behavioral	309:318	arg1	dysfunctions					320:331	neuronal and behavioral dysfunctions	296:331	neuronal and behavioral dysfunctions	296:331	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	5	108	from	responses	1318:1326	arg1	thermoregulation					1290:1305	thermoregulation	1290:1305	thermoregulation	1290:1305	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	8	109	theme	marked	1685:1690	arg1	variability					1692:1702	marked variability	1685:1702	marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates	1685:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	8	110	from	salt	1757:1760	arg1	terms					1765:1769	terms	1765:1769	terms of their effects on spontaneous abortion rates	1765:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	0	111	theme	activation	131:140	arg1	models					105:110	mouse models	99:110	mouse models of maternal immune activation	99:140	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	10	112	theme	immune	2055:2060	arg1	responses					2062:2070	varying immune responses	2047:2070	varying immune responses	2047:2070	Taken together, the present data confirm that different poly(I:C) products can induce varying immune responses and can differentially affect maternal physiology and pregnancy outcomes.					
30980948	0	113	from	responses	63:71	arg1	models					105:110	mouse models	99:110	mouse models of maternal immune activation	99:140	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	3	114	theme	I	708:708	arg1	C					710:710	I:C	708:710	I:C	708:710	The present study aimed at extending these findings to pregnant mice, while also controlling various poly(I:C) products for potential contamination with lipopolysaccharide (LPS).					
30980948	3	114	theme	I	708:708	arg1	poly					703:706	poly	703:706	various poly(I:C) products	695:720	The present study aimed at extending these findings to pregnant mice, while also controlling various poly(I:C) products for potential contamination with lipopolysaccharide (LPS).					
30980948	3	115	theme	potential	726:734	arg1	contamination					736:748	potential contamination	726:748	potential contamination with lipopolysaccharide (LPS)	726:778	The present study aimed at extending these findings to pregnant mice, while also controlling various poly(I:C) products for potential contamination with lipopolysaccharide (LPS).					
30980948	10	116	theme	maternal	2102:2109	arg1	physiology					2111:2120	maternal physiology	2102:2120	maternal physiology	2102:2120	Taken together, the present data confirm that different poly(I:C) products can induce varying immune responses and can differentially affect maternal physiology and pregnancy outcomes.					
30980948	6	117	theme	molecular	1564:1572	arg1	LMW					1582:1584	LMW	1582:1584	LMW	1582:1584	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	117	theme	molecular	1564:1572	arg1	weight					1574:1579	low molecular weight	1560:1579	low molecular weight (LMW) poly(I:C)	1560:1595	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	4	118	theme	long	1023:1026	arg1	nucleotides					1011:1021	1000-6000 nucleotides long	1001:1026	1000-6000 nucleotides long	1001:1026	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	118	theme	long	1023:1026	arg1	range					994:998	the high molecular weight range	968:998	the high molecular weight range (1000-6000 nucleotides long)	968:1027	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	3	119	theme	poly	703:706	arg1	products					713:720	various poly(I:C) products	695:720	various poly(I:C) products	695:720	The present study aimed at extending these findings to pregnant mice, while also controlling various poly(I:C) products for potential contamination with lipopolysaccharide (LPS).					
30980948	8	120	from	terms	1765:1769	arg1	batches					1722:1728	different batches	1712:1728	different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates	1712:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	8	120	from	terms	1765:1769	arg1	rates					1812:1816	spontaneous abortion rates	1791:1816	spontaneous abortion rates	1791:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	6	121	theme	maternal	1508:1515	arg1	responses					1538:1546	larger maternal and placental immune responses	1501:1546	larger maternal and placental immune responses	1501:1546	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	122	with	Consistent	1405:1414	arg1	findings					1427:1434	these findings	1421:1434	these findings	1421:1434	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	123	theme	high	1460:1463	arg1	weight					1475:1480	high molecular weight dsRNA	1460:1486	high molecular weight dsRNA (HMW)	1460:1492	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	123	theme	high	1460:1463	arg1	HMW					1489:1491	HMW	1489:1491	HMW	1489:1491	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	11	124	theme	-based	2209:2214	arg1	models					2220:2225	poly(I:C)-based MIA models	2200:2225	poly(I:C)-based MIA models	2200:2225	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	4	125	theme	immune	1096:1101	arg1	responses					1103:1111	immune responses	1096:1111	immune responses	1096:1111	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	5	126	theme	poly	1170:1173	arg1	salt					1190:1193	poly(I:C) potassium salt	1170:1193	poly(I:C) potassium salt containing larger amounts of high molecular weight fragments	1170:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	0	127	theme	pregnancy	77:85	arg1	outcomes					87:94	pregnancy outcomes	77:94	pregnancy outcomes	77:94	Influence of poly(I:C) variability on thermoregulation, immune responses and pregnancy outcomes in mouse models of maternal immune activation.					
30980948	1	128	theme	experimental	268:279	arg1	tools					281:285	experimental tools	268:285	experimental tools	268:285	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	1	128	theme	experimental	268:279	arg1	models					176:181	Maternal immune activation (MIA) models	143:181	Maternal immune activation (MIA) models that are based on administration of the viral mimetic	143:235	Maternal immune activation (MIA) models that are based on administration of the viral mimetic, poly(I:C), are widely used as experimental tools to study neuronal and behavioral dysfunctions in relation to immune-mediated neurodevelopmental disorders and mental illnesses.					
30980948	12	129	theme	quality	2434:2440	arg1	information					2442:2452	empirically acquired quality information	2413:2452	empirically acquired quality information	2413:2452	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	12	130	theme	MIA	2534:2536	arg1	models					2538:2543	poly(I:C)-based MIA models	2518:2543	poly(I:C)-based MIA models	2518:2543	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	6	131	theme	placental	1521:1529	arg1	responses					1538:1546	larger maternal and placental immune responses	1501:1546	larger maternal and placental immune responses	1501:1546	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	5	132	theme	I	1175:1175	arg1	C					1177:1177	I:C	1175:1177	I:C	1175:1177	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	5	132	theme	I	1175:1175	arg1	poly					1170:1173	poly	1170:1173	poly(I:C) potassium salt containing larger amounts of high molecular weight fragments	1170:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	8	133	theme	salt	1757:1760	arg1	batches					1722:1728	different batches	1712:1728	different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates	1712:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	11	134	theme	MIA	2216:2218	arg1	models					2220:2225	poly(I:C)-based MIA models	2200:2225	poly(I:C)-based MIA models	2200:2225	It is therefore pivotal that researchers working with poly(I:C)-based MIA models ascertain and consider the precise molecular composition and immunogenicity of the product in use.					
30980948	4	135	theme	same	886:889	arg1	vendor					891:896	the same vendor	882:896	the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	882:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	4	135	theme	same	886:889	arg1	Sigma-Aldrich					899:911	Sigma-Aldrich	899:911	Sigma-Aldrich	899:911	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	5	136	theme	immune	1311:1316	arg1	responses					1318:1326	immune responses	1311:1326	immune responses compared to batches with minimal amounts of high molecular weight fragments	1311:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	10	137	theme	present	1981:1987	arg1	data					1989:1992	the present data	1977:1992	the present data	1977:1992	Taken together, the present data confirm that different poly(I:C) products can induce varying immune responses and can differentially affect maternal physiology and pregnancy outcomes.					
30980948	6	138	theme	I	1442:1442	arg1	C					1444:1444	I:C	1442:1444	I:C	1442:1444	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	6	138	theme	I	1442:1442	arg1	poly					1437:1440	poly	1437:1440	poly(I:C) enriched for high molecular weight dsRNA (HMW)	1437:1492	Consistent with these findings, poly(I:C) enriched for high molecular weight dsRNA (HMW) caused larger maternal and placental immune responses compared to low molecular weight (LMW) poly(I:C).					
30980948	5	139	theme	molecular	1377:1385	arg1	fragments					1394:1402	high molecular weight fragments	1372:1402	high molecular weight fragments	1372:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	5	140	theme	fragments	1394:1402	arg1	amounts					1361:1367	minimal amounts	1353:1367	minimal amounts of high molecular weight fragments	1353:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	5	140	theme	fragments	1394:1402	arg1	fragments					1394:1402	high molecular weight fragments	1372:1402	high molecular weight fragments	1372:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	4	141	theme	fetal	1146:1150	arg1	brain					1152:1156	fetal brain	1146:1156	fetal brain	1146:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	12	142	theme	acquired	2425:2432	arg1	information					2442:2452	empirically acquired quality information	2413:2452	empirically acquired quality information	2413:2452	We recommend the establishment of reference databases that combine phenotype data with empirically acquired quality information, which can aid the design, implementation and interpretation of poly(I:C)-based MIA models.					
30980948	8	143	theme	abortion	1803:1810	arg1	rates					1812:1816	spontaneous abortion rates	1791:1816	spontaneous abortion rates	1791:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	7	144	theme	LPS	1648:1650	arg1	contamination					1652:1664	possible LPS contamination	1639:1664	possible LPS contamination	1639:1664	These variable effects were unrelated to possible LPS contamination.					
30980948	2	145	theme	poly	492:495	arg1	products					502:509	different poly(I:C) products	482:509	different poly(I:C) products	482:509	Evidence from investigations in non-pregnant rodents suggests that different poly(I:C) products can vary in terms of their immunogenicity, even if they are obtained from the same vendor.					
30980948	5	146	theme	minimal	1353:1359	arg1	amounts					1361:1367	minimal amounts	1353:1367	minimal amounts of high molecular weight fragments	1353:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	5	146	theme	minimal	1353:1359	arg1	fragments					1394:1402	high molecular weight fragments	1372:1402	high molecular weight fragments	1372:1402	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	8	147	from	rates	1812:1816	arg1	terms					1765:1769	terms	1765:1769	terms of their effects on spontaneous abortion rates	1765:1816	Finally, we found marked variability between different batches of the poly(I:C) potassium salt in terms of their effects on spontaneous abortion rates.					
30980948	5	148	contain	containing	1195:1204	arg2	amounts					1213:1219	larger amounts	1206:1219	larger amounts of high molecular weight fragments	1206:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	5	148	contain	containing	1195:1204	arg1	salt					1190:1193	poly(I:C) potassium salt	1170:1193	poly(I:C) potassium salt containing larger amounts of high molecular weight fragments	1170:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	5	148	contain	containing	1195:1204	arg2	fragments					1246:1254	high molecular weight fragments	1224:1254	high molecular weight fragments	1224:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30980948	4	149	theme	amount	939:944	arg1	terms					917:921	terms	917:921	terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain	917:1156	We found significant variability between different batches of poly(I:C) potassium salt obtained from the same vendor (Sigma-Aldrich) in terms of the relative amount of dsRNA fragments in the high molecular weight range (1000-6000 nucleotides long) and with regards to their effects on maternal thermoregulation and immune responses in maternal plasma, placenta and fetal brain.					
30980948	3	150	theme	present	606:612	arg1	study					614:618	The present study	602:618	The present study	602:618	The present study aimed at extending these findings to pregnant mice, while also controlling various poly(I:C) products for potential contamination with lipopolysaccharide (LPS).					
30980948	5	151	theme	potassium	1180:1188	arg1	salt					1190:1193	poly(I:C) potassium salt	1170:1193	poly(I:C) potassium salt containing larger amounts of high molecular weight fragments	1170:1254	Batches of poly(I:C) potassium salt containing larger amounts of high molecular weight fragments induced more extensive effects on thermoregulation and immune responses compared to batches with minimal amounts of high molecular weight fragments.					
30475202	4	0	theme	Gram-stain-negative	337:355	arg1	1.0-1.2 µm					394:403	1.0-1.2 µm	394:403	1.0-1.2 µm	394:403	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	4	0	theme	Gram-stain-negative	337:355	arg1	rods					388:391	Gram-stain-negative, non-spore-forming, non-motile rods	337:391	Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length)	337:438	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	4	0	theme	Gram-stain-negative	337:355	arg1	1.2-3.0 µm					418:427	1.2-3.0 µm	418:427	1.2-3.0 µm	418:427	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	14	1	theme	rRNA	1325:1328	arg1	sequences					1335:1343	16S rRNA gene sequences	1321:1343	16S rRNA gene sequences	1321:1343	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	9	2	theme	various	851:857	arg1	substrates					922:931	growth substrates	915:931	growth substrates	915:931	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	9	2	theme	various	851:857	arg1	sugars					859:864	various sugars	851:864	various sugars	851:864	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	9	2	theme	various	851:857	arg1	polysaccharides					877:891	polysaccharides	877:891	polysaccharides	877:891	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	9	2	theme	various	851:857	arg1	extract					904:910	yeast extract	898:910	yeast extract	898:910	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	3	3	theme	bacterial	203:211	arg1	strain					213:218	An aerobic bacterial strain	192:218	An aerobic bacterial strain designated AX-7T	192:235	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	12	4	theme	61.0 mol	1217:1224	arg1	%					1225:1225	61.0 mol%	1217:1225	61.0 mol%	1217:1225	The DNA G+C content was 61.0 mol%.					
30475202	12	4	theme	61.0 mol	1217:1224	arg1	content					1205:1211	The DNA G+C content	1193:1211	The DNA G+C content	1193:1211	The DNA G+C content was 61.0 mol%.					
30475202	16	5	theme	Capsulimonas	1707:1718	arg1	nov.					1736:1739	the name Capsulimonas corticalis gen. nov.	1698:1739	the name Capsulimonas corticalis gen. nov.	1698:1739	nov. of the class Armatimonadia, for which the name Capsulimonas corticalis gen. nov., sp.					
30475202	4	6	theme	AX-7T	326:330	arg1	Cells					310:314	Cells	310:314	Cells of strain AX-7T	310:330	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	11	7	theme	polar	1179:1183	arg1	lipids					1185:1190	the polar lipids	1175:1190	the polar lipids	1175:1190	Four unidentified phospholipids and 11 unidentified polar lipids constituted the polar lipids.					
30475202	16	8	theme	gen.	1731:1734	arg1	nov.					1736:1739	the name Capsulimonas corticalis gen. nov.	1698:1739	the name Capsulimonas corticalis gen. nov.	1698:1739	nov. of the class Armatimonadia, for which the name Capsulimonas corticalis gen. nov., sp.					
30475202	12	9	theme	DNA	1197:1199	arg1	%					1225:1225	61.0 mol%	1217:1225	61.0 mol%	1217:1225	The DNA G+C content was 61.0 mol%.					
30475202	12	9	theme	DNA	1197:1199	arg1	content					1205:1211	The DNA G+C content	1193:1211	The DNA G+C content	1193:1211	The DNA G+C content was 61.0 mol%.					
30475202	14	10	theme	sequence	1467:1474	arg1	similarity					1476:1485	sequence similarity	1467:1485	sequence similarity of 88.1%	1467:1494	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	2	11	theme	Armatimonadetes	175:189	arg1	Armatimonadia					147:159	the class Armatimonadia	137:159	the class Armatimonadia of the phylum Armatimonadetes	137:189	nov., of the class Armatimonadia of the phylum Armatimonadetes.					
30475202	1	12	theme	bacterial	89:97	arg1	order					99:103	a novel bacterial order	81:103	a novel bacterial order	81:103	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	3	13	theme	trunk	259:263	arg1	surface					265:271	the trunk surface	255:271	the trunk surface of a Japanese beech (Fagus crenata)	255:307	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	9	14	theme	growth	915:920	arg1	substrates					922:931	growth substrates	915:931	growth substrates	915:931	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	9	14	theme	growth	915:920	arg1	sugars					859:864	various sugars	851:864	various sugars	851:864	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	9	14	theme	growth	915:920	arg1	polysaccharides					877:891	polysaccharides	877:891	polysaccharides	877:891	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	9	14	theme	growth	915:920	arg1	extract					904:910	yeast extract	898:910	yeast extract	898:910	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	18	15	theme	105890T=NBRC	1812:1823	arg1	113044T					1825:1831	=DSM 105890T=NBRC 113044T	1807:1831	=DSM 105890T=NBRC 113044T	1807:1831	The type strain of C. corticalis is AX-7T (=DSM 105890T=NBRC 113044T).					
30475202	18	15	theme	105890T=NBRC	1812:1823	arg1	AX-7T					1800:1804	AX-7T	1800:1804	AX-7T (=DSM 105890T=NBRC 113044T)	1800:1832	The type strain of C. corticalis is AX-7T (=DSM 105890T=NBRC 113044T).					
30475202	1	16	theme	order	99:103	arg1	ord					123:125	Capsulimonadales ord	106:125	Capsulimonadales ord	106:125	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	1	16	theme	order	99:103	arg1	bacterium					67:75	an aerobic capsulated bacterium	45:75	an aerobic capsulated bacterium	45:75	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	1	16	theme	order	99:103	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	14	17	theme	rosea	1448:1452	arg1	YO-36T					1454:1459	Armatimonas rosea YO-36T	1436:1459	Armatimonas rosea YO-36T	1436:1459	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	15	18	theme	novel	1596:1600	arg1	species					1602:1608	a novel species	1594:1608	a novel species	1594:1608	Based on data from this polyphasic study, we propose that strain AX-7T represents a new genus of a novel species within the novel order Capsulimonadales ord.					
30475202	16	19	theme	Armatimonadia	1673:1685	arg1	nov.					1655:1658	nov.	1655:1658	nov.	1655:1658	nov. of the class Armatimonadia, for which the name Capsulimonas corticalis gen. nov., sp.					
30475202	3	20	theme	Japanese	278:285	arg1	beech					287:291	a Japanese beech	276:291	a Japanese beech (Fagus crenata)	276:307	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	3	20	theme	Japanese	278:285	arg1	crenata					300:306	Fagus crenata	294:306	Fagus crenata	294:306	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	4	21	theme	peritrichous	445:456	arg1	fimbriae					458:465	peritrichous fimbriae	445:465	peritrichous fimbriae	445:465	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	15	22	theme	new	1581:1583	arg1	genus					1585:1589	a new genus	1579:1589	a new genus of a novel species within the novel order Capsulimonadales ord	1579:1652	Based on data from this polyphasic study, we propose that strain AX-7T represents a new genus of a novel species within the novel order Capsulimonadales ord.					
30475202	5	23	theme	thick	533:537	arg1	layer					545:549	a thick slime layer	531:549	a thick slime layer	531:549	Cells were capsulated, and a number of them were surrounded by a thick slime layer.					
30475202	11	24	theme	unidentified	1103:1114	arg1	phospholipids					1116:1128	Four unidentified phospholipids	1098:1128	Four unidentified phospholipids	1098:1128	Four unidentified phospholipids and 11 unidentified polar lipids constituted the polar lipids.					
30475202	14	25	theme	closest	1413:1419	arg1	Armatimonadia					1394:1406	the class Armatimonadia	1384:1406	the class Armatimonadia	1384:1406	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	14	25	theme	closest	1413:1419	arg1	relative					1421:1428	its closest relative	1409:1428	its closest relative being Armatimonas rosea YO-36T	1409:1459	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	10	26	theme	fatty	1085:1089	arg1	MK-10					979:983	MK-10	979:983	MK-10	979:983	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	26	theme	fatty	1085:1089	arg1	acids					1091:1095	the major cellular fatty acids	1066:1095	the major cellular fatty acids	1066:1095	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	26	theme	fatty	1085:1089	arg1	C16 					1018:1021	C16 	1018:1021	C16 	1018:1021	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	26	theme	fatty	1085:1089	arg1	menaquinones					957:968	menaquinones MK-9 and MK-10	957:983	menaquinones	957:968	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	6	27	theme	culture	600:606	arg1	medium					608:613	the culture medium	596:613	the culture medium	596:613	During growth, large aggregates formed, and the culture medium became viscous probably owing to exopolysaccharide release from the slime layer.					
30475202	10	28	theme	respiratory	992:1002	arg1	quinones					1004:1011	the respiratory quinones	988:1011	the respiratory quinones	988:1011	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	1	29	theme	capsulated	56:65	arg1	ord					123:125	Capsulimonadales ord	106:125	Capsulimonadales ord	106:125	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	1	29	theme	capsulated	56:65	arg1	bacterium					67:75	an aerobic capsulated bacterium	45:75	an aerobic capsulated bacterium	45:75	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	1	29	theme	capsulated	56:65	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	11	30	theme	unidentified	1137:1148	arg1	lipids					1156:1161	11 unidentified polar lipids	1134:1161	11 unidentified polar lipids	1134:1161	Four unidentified phospholipids and 11 unidentified polar lipids constituted the polar lipids.					
30475202	14	31	theme	class	1388:1392	arg1	Armatimonadia					1394:1406	the class Armatimonadia	1384:1406	the class Armatimonadia	1384:1406	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	14	31	theme	class	1388:1392	arg1	relative					1421:1428	its closest relative	1409:1428	its closest relative being Armatimonas rosea YO-36T	1409:1459	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	15	32	theme	Capsulimonadales	1633:1648	arg1	ord					1650:1652	the novel order Capsulimonadales ord	1617:1652	the novel order Capsulimonadales ord	1617:1652	Based on data from this polyphasic study, we propose that strain AX-7T represents a new genus of a novel species within the novel order Capsulimonadales ord.					
30475202	0	33	theme	Capsulimonas	0:11	arg1	nov.					29:32	Capsulimonas corticalis gen. nov.	0:32	Capsulimonas corticalis gen. nov.	0:32	Capsulimonas corticalis gen. nov., sp.					
30475202	10	34	theme	Strain	934:939	arg1	AX-7T					941:945	Strain AX-7T	934:945	Strain AX-7T	934:945	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	15	35	theme	novel	1621:1625	arg1	ord					1650:1652	the novel order Capsulimonadales ord	1617:1652	the novel order Capsulimonadales ord	1617:1652	Based on data from this polyphasic study, we propose that strain AX-7T represents a new genus of a novel species within the novel order Capsulimonadales ord.					
30475202	0	36	theme	gen.	24:27	arg1	nov.					29:32	Capsulimonas corticalis gen. nov.	0:32	Capsulimonas corticalis gen. nov.	0:32	Capsulimonas corticalis gen. nov., sp.					
30475202	4	37	with	rods	388:391	arg1	fimbriae					458:465	peritrichous fimbriae	445:465	peritrichous fimbriae	445:465	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	7	38	theme	temperature	700:710	arg1	range					712:716	The temperature range	696:716	The temperature range for growth	696:727	The temperature range for growth was 10-37 °C, with an optimum at 30 °C.					
30475202	6	39	theme	slime	683:687	arg1	layer					689:693	the slime layer	679:693	the slime layer	679:693	During growth, large aggregates formed, and the culture medium became viscous probably owing to exopolysaccharide release from the slime layer.					
30475202	15	40	from	study	1532:1536	arg1	data					1506:1509	data	1506:1509	data from this polyphasic study	1506:1536	Based on data from this polyphasic study, we propose that strain AX-7T represents a new genus of a novel species within the novel order Capsulimonadales ord.					
30475202	14	41	theme	strain	1359:1364	arg1	AX-7T					1366:1370	strain AX-7T	1359:1370	strain AX-7T	1359:1370	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	9	42	used	used	846:849	arg2	AX-7T					840:844	Strain AX-7T	833:844	Strain AX-7T	833:844	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	2	43	theme	Armatimonadia	147:159	arg1	nov.					128:131	nov.	128:131	nov.	128:131	nov., of the class Armatimonadia of the phylum Armatimonadetes.					
30475202	13	44	theme	ll-diaminopimelic	1266:1282	arg1	acid					1284:1287	ll-diaminopimelic acid	1266:1287	ll-diaminopimelic acid	1266:1287	The cell-wall peptidoglycan contained ll-diaminopimelic acid.					
30475202	4	45	dep	rods	388:391	arg1	1.0-1.2 µm					394:403	1.0-1.2 µm	394:403	1.0-1.2 µm	394:403	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	4	45	dep	rods	388:391	arg1	rods					388:391	Gram-stain-negative, non-spore-forming, non-motile rods	337:391	Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length)	337:438	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	4	45	dep	rods	388:391	arg1	1.2-3.0 µm					418:427	1.2-3.0 µm	418:427	1.2-3.0 µm	418:427	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	6	46	theme	exopolysaccharide	648:664	arg1	release					666:672	exopolysaccharide release	648:672	exopolysaccharide release from the slime layer	648:693	During growth, large aggregates formed, and the culture medium became viscous probably owing to exopolysaccharide release from the slime layer.					
30475202	3	47	theme	aerobic	195:201	arg1	strain					213:218	An aerobic bacterial strain	192:218	An aerobic bacterial strain designated AX-7T	192:235	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	14	48	theme	gene	1330:1333	arg1	sequences					1335:1343	16S rRNA gene sequences	1321:1343	16S rRNA gene sequences	1321:1343	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	9	49	theme	Strain	833:838	arg1	AX-7T					840:844	Strain AX-7T	833:844	Strain AX-7T	833:844	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	15	50	theme	strain	1555:1560	arg1	AX-7T					1562:1566	strain AX-7T	1555:1566	strain AX-7T	1555:1566	Based on data from this polyphasic study, we propose that strain AX-7T represents a new genus of a novel species within the novel order Capsulimonadales ord.					
30475202	10	51	dep	C16 	1018:1021	arg1	 0					1060:1061	 0	1060:1061	 0	1060:1061	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	51	dep	C16 	1018:1021	arg1	C16 					1030:1033	C16 	1030:1033	C16 	1030:1033	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	51	dep	C16 	1018:1021	arg1	 0					1048:1049	 0	1048:1049	 0	1048:1049	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	51	dep	C16 	1018:1021	arg1	C16 					1043:1046	C16 	1043:1046	C16 	1043:1046	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	51	dep	C16 	1018:1021	arg1	C14 					1055:1058	C14 	1055:1058	C14 	1055:1058	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	14	52	theme	16S	1321:1323	arg1	sequences					1335:1343	16S rRNA gene sequences	1321:1343	16S rRNA gene sequences	1321:1343	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	16	53	theme	name	1702:1705	arg1	nov.					1736:1739	the name Capsulimonas corticalis gen. nov.	1698:1739	the name Capsulimonas corticalis gen. nov.	1698:1739	nov. of the class Armatimonadia, for which the name Capsulimonas corticalis gen. nov., sp.					
30475202	4	54	theme	strain	319:324	arg1	AX-7T					326:330	strain AX-7T	319:330	strain AX-7T	319:330	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	16	55	theme	corticalis	1720:1729	arg1	nov.					1736:1739	the name Capsulimonas corticalis gen. nov.	1698:1739	the name Capsulimonas corticalis gen. nov.	1698:1739	nov. of the class Armatimonadia, for which the name Capsulimonas corticalis gen. nov., sp.					
30475202	14	56	theme	Phylogenetic	1290:1301	arg1	analysis					1303:1310	Phylogenetic analysis	1290:1310	Phylogenetic analysis based on 16S rRNA gene sequences	1290:1343	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	10	57	theme	major	1070:1074	arg1	MK-10					979:983	MK-10	979:983	MK-10	979:983	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	57	theme	major	1070:1074	arg1	acids					1091:1095	the major cellular fatty acids	1066:1095	the major cellular fatty acids	1066:1095	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	57	theme	major	1070:1074	arg1	C16 					1018:1021	C16 	1018:1021	C16 	1018:1021	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	57	theme	major	1070:1074	arg1	menaquinones					957:968	menaquinones MK-9 and MK-10	957:983	menaquinones	957:968	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	13	58	theme	cell-wall	1232:1240	arg1	peptidoglycan					1242:1254	The cell-wall peptidoglycan	1228:1254	The cell-wall peptidoglycan	1228:1254	The cell-wall peptidoglycan contained ll-diaminopimelic acid.					
30475202	4	59	from	1.2-3.0 µm	418:427	arg1	length					432:437	length	432:437	length	432:437	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	15	60	theme	polyphasic	1521:1530	arg1	study					1532:1536	this polyphasic study	1516:1536	this polyphasic study	1516:1536	Based on data from this polyphasic study, we propose that strain AX-7T represents a new genus of a novel species within the novel order Capsulimonadales ord.					
30475202	13	61	contain	contained	1256:1264	arg1	peptidoglycan					1242:1254	The cell-wall peptidoglycan	1228:1254	The cell-wall peptidoglycan	1228:1254	The cell-wall peptidoglycan contained ll-diaminopimelic acid.					
30475202	13	61	contain	contained	1256:1264	arg2	acid					1284:1287	ll-diaminopimelic acid	1266:1287	ll-diaminopimelic acid	1266:1287	The cell-wall peptidoglycan contained ll-diaminopimelic acid.					
30475202	9	62	theme	yeast	898:902	arg1	substrates					922:931	growth substrates	915:931	growth substrates	915:931	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	9	62	theme	yeast	898:902	arg1	sugars					859:864	various sugars	851:864	various sugars	851:864	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	9	62	theme	yeast	898:902	arg1	polysaccharides					877:891	polysaccharides	877:891	polysaccharides	877:891	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	9	62	theme	yeast	898:902	arg1	extract					904:910	yeast extract	898:910	yeast extract	898:910	Strain AX-7T used various sugars, including polysaccharides, and yeast extract as growth substrates.					
30475202	4	63	from	1.0-1.2 µm	394:403	arg1	width					408:412	width	408:412	width	408:412	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	18	64	theme	corticalis	1786:1795	arg1	AX-7T					1800:1804	AX-7T	1800:1804	AX-7T (=DSM 105890T=NBRC 113044T)	1800:1832	The type strain of C. corticalis is AX-7T (=DSM 105890T=NBRC 113044T).					
30475202	18	64	theme	corticalis	1786:1795	arg1	strain					1773:1778	The type strain	1764:1778	The type strain of C. corticalis	1764:1795	The type strain of C. corticalis is AX-7T (=DSM 105890T=NBRC 113044T).					
30475202	1	65	theme	novel	83:87	arg1	order					99:103	a novel bacterial order	81:103	a novel bacterial order	81:103	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	12	66	theme	G+C	1201:1203	arg1	%					1225:1225	61.0 mol%	1217:1225	61.0 mol%	1217:1225	The DNA G+C content was 61.0 mol%.					
30475202	12	66	theme	G+C	1201:1203	arg1	content					1205:1211	The DNA G+C content	1193:1211	The DNA G+C content	1193:1211	The DNA G+C content was 61.0 mol%.					
30475202	10	67	theme	cellular	1076:1083	arg1	MK-10					979:983	MK-10	979:983	MK-10	979:983	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	67	theme	cellular	1076:1083	arg1	acids					1091:1095	the major cellular fatty acids	1066:1095	the major cellular fatty acids	1066:1095	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	67	theme	cellular	1076:1083	arg1	C16 					1018:1021	C16 	1018:1021	C16 	1018:1021	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	67	theme	cellular	1076:1083	arg1	menaquinones					957:968	menaquinones MK-9 and MK-10	957:983	menaquinones	957:968	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	5	68	theme	them	507:510	arg1	number					497:502	a number	495:502	a number of them	495:510	Cells were capsulated, and a number of them were surrounded by a thick slime layer.					
30475202	16	69	theme	class	1667:1671	arg1	Armatimonadia					1673:1685	the class Armatimonadia	1663:1685	the class Armatimonadia	1663:1685	nov. of the class Armatimonadia, for which the name Capsulimonas corticalis gen. nov., sp.					
30475202	4	70	dep	Gram-stain-negative	337:355	arg1	non-motile					377:386	non-motile	377:386	non-motile	377:386	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	4	70	dep	Gram-stain-negative	337:355	arg1	non-spore-forming					358:374	non-spore-forming	358:374	non-spore-forming	358:374	Cells of strain AX-7T were Gram-stain-negative, non-spore-forming, non-motile rods (1.0-1.2 µm in width and 1.2-3.0 µm in length) with peritrichous fimbriae.					
30475202	18	71	theme	=DSM	1807:1810	arg1	113044T					1825:1831	=DSM 105890T=NBRC 113044T	1807:1831	=DSM 105890T=NBRC 113044T	1807:1831	The type strain of C. corticalis is AX-7T (=DSM 105890T=NBRC 113044T).					
30475202	18	71	theme	=DSM	1807:1810	arg1	AX-7T					1800:1804	AX-7T	1800:1804	AX-7T (=DSM 105890T=NBRC 113044T)	1800:1832	The type strain of C. corticalis is AX-7T (=DSM 105890T=NBRC 113044T).					
30475202	1	72	theme	Capsulimonadales	106:121	arg1	ord					123:125	Capsulimonadales ord	106:125	Capsulimonadales ord	106:125	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	1	72	theme	Capsulimonadales	106:121	arg1	bacterium					67:75	an aerobic capsulated bacterium	45:75	an aerobic capsulated bacterium	45:75	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	3	73	theme	Fagus	294:298	arg1	beech					287:291	a Japanese beech	276:291	a Japanese beech (Fagus crenata)	276:307	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	3	73	theme	Fagus	294:298	arg1	crenata					300:306	Fagus crenata	294:306	Fagus crenata	294:306	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	14	74	theme	Armatimonas	1436:1446	arg1	YO-36T					1454:1459	Armatimonas rosea YO-36T	1436:1459	Armatimonas rosea YO-36T	1436:1459	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	6	75	theme	large	567:571	arg1	aggregates					573:582	large aggregates	567:582	large aggregates	567:582	During growth, large aggregates formed, and the culture medium became viscous probably owing to exopolysaccharide release from the slime layer.					
30475202	3	76	theme	beech	287:291	arg1	surface					265:271	the trunk surface	255:271	the trunk surface of a Japanese beech (Fagus crenata)	255:307	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	10	77	contain	contained	947:955	arg2	menaquinones					957:968	menaquinones MK-9 and MK-10	957:983	menaquinones	957:968	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	77	contain	contained	947:955	arg1	AX-7T					941:945	Strain AX-7T	934:945	Strain AX-7T	934:945	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	77	contain	contained	947:955	arg2	acids					1091:1095	the major cellular fatty acids	1066:1095	the major cellular fatty acids	1066:1095	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	77	contain	contained	947:955	arg2	C16 					1018:1021	C16 	1018:1021	C16 	1018:1021	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	10	77	contain	contained	947:955	arg2	MK-10					979:983	MK-10	979:983	MK-10	979:983	Strain AX-7T contained menaquinones MK-9 and MK-10 as the respiratory quinones, and C16 : 1ω5c, C16 : 1ω11c, C16 : 0 and C14 : 0 as the major cellular fatty acids.					
30475202	5	78	theme	slime	539:543	arg1	layer					545:549	a thick slime layer	531:549	a thick slime layer	531:549	Cells were capsulated, and a number of them were surrounded by a thick slime layer.					
30475202	8	79	theme	pH	773:774	arg1	5.0-7.0					797:803	5.0-7.0	797:803	5.0-7.0	797:803	The pH range for growth was 5.0-7.0, with an optimum at pH 6.0.					
30475202	8	79	theme	pH	773:774	arg1	range					776:780	The pH range	769:780	The pH range for growth	769:791	The pH range for growth was 5.0-7.0, with an optimum at pH 6.0.					
30475202	14	80	theme	%	1494:1494	arg1	similarity					1476:1485	sequence similarity	1467:1485	sequence similarity of 88.1%	1467:1494	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AX-7T belonged to the class Armatimonadia, its closest relative being Armatimonas rosea YO-36T, with sequence similarity of 88.1%.					
30475202	1	81	theme	aerobic	48:54	arg1	ord					123:125	Capsulimonadales ord	106:125	Capsulimonadales ord	106:125	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	1	81	theme	aerobic	48:54	arg1	bacterium					67:75	an aerobic capsulated bacterium	45:75	an aerobic capsulated bacterium	45:75	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	1	81	theme	aerobic	48:54	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov., an aerobic capsulated bacterium, of a novel bacterial order, Capsulimonadales ord.					
30475202	16	82	dep	sp	1742:1743	arg1	nov.					1655:1658	nov.	1655:1658	nov.	1655:1658	nov. of the class Armatimonadia, for which the name Capsulimonas corticalis gen. nov., sp.					
30475202	15	83	theme	order	1627:1631	arg1	ord					1650:1652	the novel order Capsulimonadales ord	1617:1652	the novel order Capsulimonadales ord	1617:1652	Based on data from this polyphasic study, we propose that strain AX-7T represents a new genus of a novel species within the novel order Capsulimonadales ord.					
30475202	0	84	theme	corticalis	13:22	arg1	nov.					29:32	Capsulimonas corticalis gen. nov.	0:32	Capsulimonas corticalis gen. nov.	0:32	Capsulimonas corticalis gen. nov., sp.					
30475202	18	85	theme	type	1768:1771	arg1	AX-7T					1800:1804	AX-7T	1800:1804	AX-7T (=DSM 105890T=NBRC 113044T)	1800:1832	The type strain of C. corticalis is AX-7T (=DSM 105890T=NBRC 113044T).					
30475202	18	85	theme	type	1768:1771	arg1	strain					1773:1778	The type strain	1764:1778	The type strain of C. corticalis	1764:1795	The type strain of C. corticalis is AX-7T (=DSM 105890T=NBRC 113044T).					
30475202	8	86	from	pH	825:826	arg1	optimum					814:820	an optimum	811:820	an optimum at pH 6.0	811:830	The pH range for growth was 5.0-7.0, with an optimum at pH 6.0.					
30475202	11	87	theme	polar	1150:1154	arg1	lipids					1156:1161	11 unidentified polar lipids	1134:1161	11 unidentified polar lipids	1134:1161	Four unidentified phospholipids and 11 unidentified polar lipids constituted the polar lipids.					
30475202	2	88	theme	class	141:145	arg1	Armatimonadia					147:159	the class Armatimonadia	137:159	the class Armatimonadia of the phylum Armatimonadetes	137:189	nov., of the class Armatimonadia of the phylum Armatimonadetes.					
30475202	15	89	theme	species	1602:1608	arg1	genus					1585:1589	a new genus	1579:1589	a new genus of a novel species within the novel order Capsulimonadales ord	1579:1652	Based on data from this polyphasic study, we propose that strain AX-7T represents a new genus of a novel species within the novel order Capsulimonadales ord.					
30475202	0	90	dep	sp	35:36	arg1	nov.					29:32	Capsulimonas corticalis gen. nov.	0:32	Capsulimonas corticalis gen. nov.	0:32	Capsulimonas corticalis gen. nov., sp.					
30475202	6	91	from	layer	689:693	arg1	release					666:672	exopolysaccharide release	648:672	exopolysaccharide release from the slime layer	648:693	During growth, large aggregates formed, and the culture medium became viscous probably owing to exopolysaccharide release from the slime layer.					
30475202	3	92	attach	isolated	241:248	arg1	surface					265:271	the trunk surface	255:271	the trunk surface of a Japanese beech (Fagus crenata)	255:307	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	3	92	attach	isolated	241:248	arg2	strain					213:218	An aerobic bacterial strain	192:218	An aerobic bacterial strain designated AX-7T	192:235	An aerobic bacterial strain designated AX-7T was isolated from the trunk surface of a Japanese beech (Fagus crenata).					
30475202	2	93	theme	phylum	168:173	arg1	Armatimonadetes					175:189	the phylum Armatimonadetes	164:189	the phylum Armatimonadetes	164:189	nov., of the class Armatimonadia of the phylum Armatimonadetes.					
30475202	7	94	from	30 °C	762:766	arg1	optimum					751:757	an optimum	748:757	an optimum at 30 °C	748:766	The temperature range for growth was 10-37 °C, with an optimum at 30 °C.					
30348015	12	0	theme	cytocompatible	1923:1936	arg1	BG					1903:1904	BG	1903:1904	BG	1903:1904	BG could serve as a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair.					
30348015	12	0	theme	cytocompatible	1923:1936	arg1	basis					1938:1942	a cytocompatible basis	1921:1942	a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair	1921:2015	BG could serve as a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair.					
30348015	4	1	from	effects	795:801	arg1	chondrogenesis					806:819	chondrogenesis	806:819	chondrogenesis	806:819	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	6	2	theme	X-ray	1084:1088	arg1	diffraction					1090:1100	X-ray diffraction	1084:1100	X-ray diffraction (XRD)	1084:1106	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	6	2	theme	X-ray	1084:1088	arg1	XRD					1103:1105	XRD	1103:1105	XRD	1103:1105	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	11	3	theme	protein	1765:1771	arg1	expression					1773:1782	Chondrocyte protein expression	1753:1782	Chondrocyte protein expression for cartilage ECM proteins	1753:1809	Chondrocyte protein expression for cartilage ECM proteins indicated that the chondrocytes maintained their differentiated phenotype in the scaffolds.					
30348015	6	4	theme	electron	1057:1064	arg1	SEM					1078:1080	SEM	1078:1080	SEM	1078:1080	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	6	4	theme	electron	1057:1064	arg1	microscopy					1066:1075	scanning electron microscopy	1048:1075	scanning electron microscopy (SEM)	1048:1081	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	4	5	theme	/1393BG	704:710	arg1	scaffolds					712:720	composite porous poly(L-lactide) (PLLA)/1393BG scaffolds	665:720	composite porous poly(L-lactide) (PLLA)/1393BG scaffolds	665:720	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	12	6	theme	future	1948:1953	arg1	composites					1964:1973	future scaffold composites	1948:1973	future scaffold composites for osteochondral cartilage defect repair	1948:2015	BG could serve as a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair.					
30348015	0	7	theme	induced	110:116	arg1	separation					124:133	thermally induced phase separation	100:133	thermally induced phase separation	100:133	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	7	8	theme	SOX9	1319:1322	arg1	attachment					1278:1287	attachment	1278:1287	attachment	1278:1287	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	8	theme	SOX9	1319:1322	arg1	survival					1268:1275	cell survival	1263:1275	cell survival	1263:1275	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	8	theme	SOX9	1319:1322	arg1	expression					1305:1314	expression	1305:1314	expression	1305:1314	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	8	theme	SOX9	1319:1322	arg1	morphology					1290:1299	morphology	1290:1299	morphology	1290:1299	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	9	theme	cell	1263:1266	arg1	survival					1268:1275	cell survival	1263:1275	cell survival	1263:1275	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	3	10	theme	stimulatory	573:583	arg1	effects					585:591	stimulatory effects	573:591	stimulatory effects	573:591	Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis.					
30348015	2	11	from	Implantation	336:347	arg1	combination					379:389	combination	379:389	combination with a biomimetic biomaterial	379:419	Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair.					
30348015	4	12	theme	work	628:631	arg1	aim					616:618	The aim	612:618	The aim of this work	612:631	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	9	13	theme	scaffold	1548:1555	arg1	parts					1535:1539	inner parts	1529:1539	inner parts of the scaffold	1529:1555	Also inner parts of the scaffold were colonized by chondrocytes synthesizing an ECM which contained glycosaminoglycans.					
30348015	4	14	theme	phase	744:748	arg1	TIPS					762:765	TIPS	762:765	TIPS	762:765	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	4	14	theme	phase	744:748	arg1	separation					750:759	phase separation	744:759	thermally induced phase separation (TIPS) technique	726:776	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	4	15	theme	induced	736:742	arg1	technique					768:776	thermally induced phase separation (TIPS) technique	726:776	thermally induced phase separation (TIPS) technique	726:776	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	7	16	theme	nasoseptal	1169:1178	arg1	chondrocytes					1180:1191	Human nasoseptal chondrocytes	1163:1191	Human nasoseptal chondrocytes	1163:1191	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	6	17	theme	differential	1112:1123	arg1	analysis					1147:1154	differential scanning calorimetric analysis	1112:1154	differential scanning calorimetric analysis (DSC)	1112:1160	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	6	17	theme	differential	1112:1123	arg1	DSC					1157:1159	DSC	1157:1159	DSC	1157:1159	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	2	18	theme	cartilage	464:472	arg1	repair					474:479	cartilage repair	464:479	cartilage repair	464:479	Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair.					
30348015	8	19	theme	chondrocytes	1427:1438	arg1	majority					1415:1422	The majority	1411:1422	The majority of chondrocytes	1411:1438	The majority of chondrocytes survived on all PLLA scaffolds functionalized with BG for the whole culture period.					
30348015	6	20	theme	scanning	1048:1055	arg1	SEM					1078:1080	SEM	1078:1080	SEM	1078:1080	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	6	20	theme	scanning	1048:1055	arg1	microscopy					1066:1075	scanning electron microscopy	1048:1075	scanning electron microscopy (SEM)	1048:1081	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	0	21	theme	phase	118:122	arg1	separation					124:133	thermally induced phase separation	100:133	thermally induced phase separation	100:133	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	8	22	theme	culture	1508:1514	arg1	period					1516:1521	the whole culture period	1498:1521	the whole culture period	1498:1521	The majority of chondrocytes survived on all PLLA scaffolds functionalized with BG for the whole culture period.					
30348015	12	23	theme	scaffold	1955:1962	arg1	composites					1964:1973	future scaffold composites	1948:1973	future scaffold composites for osteochondral cartilage defect repair	1948:2015	BG could serve as a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair.					
30348015	7	24	theme	Human	1163:1167	arg1	chondrocytes					1180:1191	Human nasoseptal chondrocytes	1163:1191	Human nasoseptal chondrocytes	1163:1191	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	2	25	theme	promising	434:442	arg1	strategy					444:451	a promising strategy	432:451	a promising strategy to support cartilage repair	432:479	Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair.					
30348015	0	26	theme	nasoseptal	6:15	arg1	chondrocytes					17:28	Human nasoseptal chondrocytes	0:28	Human nasoseptal chondrocytes	0:28	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	8	27	theme	whole	1502:1506	arg1	period					1516:1521	the whole culture period	1498:1521	the whole culture period	1498:1521	The majority of chondrocytes survived on all PLLA scaffolds functionalized with BG for the whole culture period.					
30348015	12	28	theme	osteochondral	1979:1991	arg1	repair					2010:2015	osteochondral cartilage defect repair	1979:2015	osteochondral cartilage defect repair	1979:2015	BG could serve as a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair.					
30348015	1	29	theme	cartilage	197:205	arg1	Damage					179:184	Damage	179:184	Damage of hyaline cartilage such as nasoseptal cartilage	179:234	Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity.					
30348015	0	30	theme	Human	0:4	arg1	chondrocytes					17:28	Human nasoseptal chondrocytes	0:28	Human nasoseptal chondrocytes	0:28	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	11	31	theme	ECM	1798:1800	arg1	proteins					1802:1809	cartilage ECM proteins	1788:1809	cartilage ECM proteins	1788:1809	Chondrocyte protein expression for cartilage ECM proteins indicated that the chondrocytes maintained their differentiated phenotype in the scaffolds.					
30348015	10	32	theme	gene	1674:1677	arg1	expression					1679:1688	Type II collagen and aggrecan gene expression	1644:1688	Type II collagen and aggrecan gene expression	1644:1688	Type II collagen and aggrecan gene expression increased significantly in 1% BG scaffolds during the culture.					
30348015	4	33	theme	separation	750:759	arg1	technique					768:776	thermally induced phase separation (TIPS) technique	726:776	thermally induced phase separation (TIPS) technique	726:776	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	9	34	contain	contained	1614:1622	arg1	ECM					1604:1606	an ECM	1601:1606	an ECM which contained glycosaminoglycans	1601:1641	Also inner parts of the scaffold were colonized by chondrocytes synthesizing an ECM which contained glycosaminoglycans.					
30348015	9	34	contain	contained	1614:1622	arg2	glycosaminoglycans					1624:1641	glycosaminoglycans	1624:1641	glycosaminoglycans	1624:1641	Also inner parts of the scaffold were colonized by chondrocytes synthesizing an ECM which contained glycosaminoglycans.					
30348015	1	35	theme	low	305:307	arg1	capacity					326:333	its low intrinsic repair capacity	301:333	its low intrinsic repair capacity	301:333	Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity.					
30348015	10	36	theme	aggrecan	1665:1672	arg1	expression					1679:1688	Type II collagen and aggrecan gene expression	1644:1688	Type II collagen and aggrecan gene expression	1644:1688	Type II collagen and aggrecan gene expression increased significantly in 1% BG scaffolds during the culture.					
30348015	5	37	theme	TIPS	920:923	arg1	technique					925:933	TIPS technique	920:933	TIPS technique starting from a ternary solution (polymer/solvent/non-solvent) in a single step	920:1013	The PLLA scaffolds without or with 1, 2.5, 5% BG1393 were prepared via TIPS technique starting from a ternary solution (polymer/solvent/non-solvent) in a single step.					
30348015	0	38	dep	scaffolds	78:86	arg1	produced					88:95	produced	88:95	produced by thermally induced phase separation	88:133	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	0	38	dep	scaffolds	78:86	arg1	supplemented					139:150	supplemented	139:150	supplemented with bioactive glass 1393	139:176	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	7	39	theme	extracellular	1347:1359	arg1	ECM					1379:1381	ECM	1379:1381	ECM	1379:1381	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	39	theme	extracellular	1347:1359	arg1	matrix					1371:1376	cartilage-specific extracellular cartilage matrix	1328:1376	cartilage-specific extracellular cartilage matrix (ECM)	1328:1382	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	4	40	theme	composite	665:673	arg1	L-lactide					687:695	L-lactide	687:695	L-lactide	687:695	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	4	40	theme	composite	665:673	arg1	PLLA					699:702	PLLA	699:702	PLLA	699:702	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	4	40	theme	composite	665:673	arg1	poly					682:685	composite porous poly	665:685	composite porous poly(L-lactide) (PLLA)/1393BG scaffolds	665:720	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	12	41	theme	defect	2003:2008	arg1	repair					2010:2015	osteochondral cartilage defect repair	1979:2015	osteochondral cartilage defect repair	1979:2015	BG could serve as a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair.					
30348015	1	42	theme	intrinsic	309:317	arg1	capacity					326:333	its low intrinsic repair capacity	301:333	its low intrinsic repair capacity	301:333	Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity.					
30348015	10	43	theme	BG	1720:1721	arg1	scaffolds					1723:1731	1% BG scaffolds	1717:1731	1% BG scaffolds	1717:1731	Type II collagen and aggrecan gene expression increased significantly in 1% BG scaffolds during the culture.					
30348015	6	44	theme	scanning	1125:1132	arg1	analysis					1147:1154	differential scanning calorimetric analysis	1112:1154	differential scanning calorimetric analysis (DSC)	1112:1160	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	6	44	theme	scanning	1125:1132	arg1	DSC					1157:1159	DSC	1157:1159	DSC	1157:1159	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	1	45	theme	nasoseptal	215:224	arg1	cartilage					226:234	nasoseptal cartilage	215:234	nasoseptal cartilage	215:234	Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity.					
30348015	0	46	theme	differentiated	45:58	arg1	phenotype					60:68	their differentiated phenotype	39:68	their differentiated phenotype	39:68	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	1	47	theme	repair	319:324	arg1	capacity					326:333	its low intrinsic repair capacity	301:333	its low intrinsic repair capacity	301:333	Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity.					
30348015	0	48	theme	bioactive	157:165	arg1	1393					173:176	bioactive glass 1393	157:176	bioactive glass 1393	157:176	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	10	49	theme	collagen	1652:1659	arg1	expression					1679:1688	Type II collagen and aggrecan gene expression	1644:1688	Type II collagen and aggrecan gene expression	1644:1688	Type II collagen and aggrecan gene expression increased significantly in 1% BG scaffolds during the culture.					
30348015	4	50	theme	chondrocytes	835:846	arg1	effects					795:801	their effects	789:801	their effects on chondrogenesis of nasoseptal chondrocytes	789:846	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	4	51	theme	poly	682:685	arg1	scaffolds					712:720	composite porous poly(L-lactide) (PLLA)/1393BG scaffolds	665:720	composite porous poly(L-lactide) (PLLA)/1393BG scaffolds	665:720	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	10	52	theme	Type	1644:1647	arg1	collagen					1652:1659	Type II collagen	1644:1659	Type II collagen	1644:1659	Type II collagen and aggrecan gene expression increased significantly in 1% BG scaffolds during the culture.					
30348015	7	53	theme	matrix	1371:1376	arg1	attachment					1278:1287	attachment	1278:1287	attachment	1278:1287	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	53	theme	matrix	1371:1376	arg1	survival					1268:1275	cell survival	1263:1275	cell survival	1263:1275	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	53	theme	matrix	1371:1376	arg1	expression					1305:1314	expression	1305:1314	expression	1305:1314	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	53	theme	matrix	1371:1376	arg1	morphology					1290:1299	morphology	1290:1299	morphology	1290:1299	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	2	54	with	combination	379:389	arg1	biomaterial					409:419	a biomimetic biomaterial	396:419	a biomimetic biomaterial	396:419	Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair.					
30348015	4	55	theme	nasoseptal	824:833	arg1	chondrocytes					835:846	nasoseptal chondrocytes	824:846	nasoseptal chondrocytes	824:846	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	4	56	theme	porous	675:680	arg1	L-lactide					687:695	L-lactide	687:695	L-lactide	687:695	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	4	56	theme	porous	675:680	arg1	PLLA					699:702	PLLA	699:702	PLLA	699:702	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	4	56	theme	porous	675:680	arg1	poly					682:685	composite porous poly	665:685	composite porous poly(L-lactide) (PLLA)/1393BG scaffolds	665:720	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	10	57	theme	%	1718:1718	arg1	scaffolds					1723:1731	1% BG scaffolds	1717:1731	1% BG scaffolds	1717:1731	Type II collagen and aggrecan gene expression increased significantly in 1% BG scaffolds during the culture.					
30348015	3	58	theme	bone	515:518	arg1	engineering					527:537	bone tissue engineering	515:537	bone tissue engineering	515:537	Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis.					
30348015	10	59	theme	1	1717:1717	arg1	%					1718:1718	%	1718:1718	%	1718:1718	Type II collagen and aggrecan gene expression increased significantly in 1% BG scaffolds during the culture.					
30348015	11	60	theme	Chondrocyte	1753:1763	arg1	expression					1773:1782	Chondrocyte protein expression	1753:1782	Chondrocyte protein expression for cartilage ECM proteins	1753:1809	Chondrocyte protein expression for cartilage ECM proteins indicated that the chondrocytes maintained their differentiated phenotype in the scaffolds.					
30348015	0	61	theme	glass	167:171	arg1	1393					173:176	bioactive glass 1393	157:176	bioactive glass 1393	157:176	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	7	62	theme	cartilage-specific	1328:1345	arg1	ECM					1379:1381	ECM	1379:1381	ECM	1379:1381	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	62	theme	cartilage-specific	1328:1345	arg1	matrix					1371:1376	cartilage-specific extracellular cartilage matrix	1328:1376	cartilage-specific extracellular cartilage matrix (ECM)	1328:1382	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	3	63	theme	bioactive	540:548	arg1	BG					557:558	BG	557:558	BG	557:558	Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis.					
30348015	3	63	theme	bioactive	540:548	arg1	glass					550:554	bioactive glass	540:554	bioactive glass (BG)	540:559	Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis.					
30348015	12	64	theme	cartilage	1993:2001	arg1	repair					2010:2015	osteochondral cartilage defect repair	1979:2015	osteochondral cartilage defect repair	1979:2015	BG could serve as a cytocompatible basis for future scaffold composites for osteochondral cartilage defect repair.					
30348015	4	65	dep	produce	640:646	arg1	characterize					652:663	characterize	652:663	characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique	652:776	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	4	65	dep	produce	640:646	arg1	assess					782:787	assess	782:787	assess their effects on chondrogenesis of nasoseptal chondrocytes	782:846	The aim of this work was to produce and characterize composite porous poly(L-lactide) (PLLA)/1393BG scaffolds via thermally induced phase separation (TIPS) technique and assess their effects on chondrogenesis of nasoseptal chondrocytes.					
30348015	7	66	theme	cartilage	1361:1369	arg1	ECM					1379:1381	ECM	1379:1381	ECM	1379:1381	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	66	theme	cartilage	1361:1369	arg1	matrix					1371:1376	cartilage-specific extracellular cartilage matrix	1328:1376	cartilage-specific extracellular cartilage matrix (ECM)	1328:1382	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	2	67	theme	chondrocytes	363:374	arg1	Implantation					336:347	Implantation	336:347	Implantation of autologous chondrocytes in combination with a biomimetic biomaterial	336:419	Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair.					
30348015	6	68	theme	calorimetric	1134:1145	arg1	analysis					1147:1154	differential scanning calorimetric analysis	1112:1154	differential scanning calorimetric analysis (DSC)	1112:1160	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	6	68	theme	calorimetric	1134:1145	arg1	DSC					1157:1159	DSC	1157:1159	DSC	1157:1159	Scaffolds were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and differential scanning calorimetric analysis (DSC).					
30348015	1	69	theme	proper	245:250	arg1	reconstruction					252:265	proper reconstruction	245:265	proper reconstruction	245:265	Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity.					
30348015	9	70	theme	inner	1529:1533	arg1	parts					1535:1539	inner parts	1529:1539	inner parts of the scaffold	1529:1555	Also inner parts of the scaffold were colonized by chondrocytes synthesizing an ECM which contained glycosaminoglycans.					
30348015	8	71	theme	PLLA	1456:1459	arg1	scaffolds					1461:1469	all PLLA scaffolds	1452:1469	all PLLA scaffolds functionalized with BG for the whole culture period	1452:1521	The majority of chondrocytes survived on all PLLA scaffolds functionalized with BG for the whole culture period.					
30348015	5	72	theme	ternary	951:957	arg1	solution					959:966	a ternary solution	949:966	a ternary solution (polymer/solvent/non-solvent)	949:996	The PLLA scaffolds without or with 1, 2.5, 5% BG1393 were prepared via TIPS technique starting from a ternary solution (polymer/solvent/non-solvent) in a single step.					
30348015	5	72	theme	ternary	951:957	arg1	polymer/solvent/non-solvent					969:995	polymer/solvent/non-solvent	969:995	polymer/solvent/non-solvent	969:995	The PLLA scaffolds without or with 1, 2.5, 5% BG1393 were prepared via TIPS technique starting from a ternary solution (polymer/solvent/non-solvent) in a single step.					
30348015	2	73	theme	autologous	352:361	arg1	chondrocytes					363:374	autologous chondrocytes	352:374	autologous chondrocytes	352:374	Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair.					
30348015	3	74	theme	tissue	520:525	arg1	engineering					527:537	bone tissue engineering	515:537	bone tissue engineering	515:537	Despite so far mostly tested for bone tissue engineering, bioactive glass (BG) could exert stimulatory effects on chondrogenesis.					
30348015	7	75	dep	%	1236:1236	arg1	2.5					1233:1235	2.5	1233:1235	2.5	1233:1235	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	75	dep	%	1236:1236	arg1	1					1227:1227	1	1227:1227	1	1227:1227	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	7	76	dep	SOX9	1319:1322	arg1	components					1384:1393	components	1384:1393	components	1384:1393	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	11	77	theme	cartilage	1788:1796	arg1	proteins					1802:1809	cartilage ECM proteins	1788:1809	cartilage ECM proteins	1788:1809	Chondrocyte protein expression for cartilage ECM proteins indicated that the chondrocytes maintained their differentiated phenotype in the scaffolds.					
30348015	5	78	theme	PLLA	853:856	arg1	scaffolds					858:866	The PLLA scaffolds	849:866	The PLLA scaffolds without or with 1, 2.5, 5% BG1393	849:900	The PLLA scaffolds without or with 1, 2.5, 5% BG1393 were prepared via TIPS technique starting from a ternary solution (polymer/solvent/non-solvent) in a single step.					
30348015	7	79	dep	BG	1238:1239	arg1	%					1236:1236	%	1236:1236	%	1236:1236	Human nasoseptal chondrocytes were seeded on the scaffolds with 1 and 2.5% BG for 7 and 14 days and cell survival, attachment, morphology and expression of SOX9 and cartilage-specific extracellular cartilage matrix (ECM) components were monitored.					
30348015	1	80	theme	hyaline	189:195	arg1	cartilage					197:205	hyaline cartilage	189:205	hyaline cartilage	189:205	Damage of hyaline cartilage such as nasoseptal cartilage requires proper reconstruction, which remains challenging due to its low intrinsic repair capacity.					
30348015	0	81	theme	PLLA	73:76	arg1	scaffolds					78:86	PLLA scaffolds	73:86	PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393	73:176	Human nasoseptal chondrocytes maintain their differentiated phenotype on PLLA scaffolds produced by thermally induced phase separation and supplemented with bioactive glass 1393.					
30348015	5	82	theme	single	1003:1008	arg1	step					1010:1013	a single step	1001:1013	a single step	1001:1013	The PLLA scaffolds without or with 1, 2.5, 5% BG1393 were prepared via TIPS technique starting from a ternary solution (polymer/solvent/non-solvent) in a single step.					
30348015	2	83	theme	biomimetic	398:407	arg1	biomaterial					409:419	a biomimetic biomaterial	396:419	a biomimetic biomaterial	396:419	Implantation of autologous chondrocytes in combination with a biomimetic biomaterial represents a promising strategy to support cartilage repair.					
30348015	11	84	theme	differentiated	1860:1873	arg1	phenotype					1875:1883	their differentiated phenotype	1854:1883	their differentiated phenotype	1854:1883	Chondrocyte protein expression for cartilage ECM proteins indicated that the chondrocytes maintained their differentiated phenotype in the scaffolds.					
31726154	9	0	theme	thermal	1261:1267	arg1	properties					1269:1278	thermal properties	1261:1278	thermal properties	1261:1278	Morphology, chemical composition, crystallinity and thermal properties of the films evidenced that TiO2 was better dispersed in both matrices at 1 wt%.					
31726154	7	1	theme	gelatin-TiO2	969:980	arg1	films					982:986	the gelatin-TiO2 films	965:986	the gelatin-TiO2 films	965:986	Moisture content, water solubility and WVP of the gelatin-TiO2 films were influenced by the incorporation of TiO2, while HPMC-TiO2 films were not.					
31726154	3	2	theme	forming	496:502	arg1	solutions					504:512	film forming solutions	491:512	film forming solutions	491:512	The aim of this work was to study the dispersion of TiO2 (0-2 wt%) incorporated in the hydroxypropyl methylcellulose (HPMC-TiO2) and gelatin (gelatin-TiO2) film forming solutions.					
31726154	10	3	theme	microstructural	1487:1501	arg1	reorganization					1503:1516	a microstructural reorganization	1485:1516	a microstructural reorganization in the matrices	1485:1532	It was also concluded that TiO2 aggregation generated more biphasic regions in HPMC than generated in gelatin, which caused a microstructural reorganization in the matrices.					
31726154	4	4	theme	Particle	515:522	arg1	size					524:527	Particle size	515:527	Particle size	515:527	Particle size and zeta potential of TiO2 nanoparticles were investigated.					
31726154	7	5	theme	water	937:941	arg1	solubility					943:952	water solubility	937:952	water solubility	937:952	Moisture content, water solubility and WVP of the gelatin-TiO2 films were influenced by the incorporation of TiO2, while HPMC-TiO2 films were not.					
31726154	3	6	dep	methylcellulose	436:450	arg1	solutions					504:512	film forming solutions	491:512	film forming solutions	491:512	The aim of this work was to study the dispersion of TiO2 (0-2 wt%) incorporated in the hydroxypropyl methylcellulose (HPMC-TiO2) and gelatin (gelatin-TiO2) film forming solutions.					
31726154	2	7	from	tendency	208:215	arg1	nanocomposites					252:265	biopolymer-based nanocomposites	235:265	biopolymer-based nanocomposites	235:265	However, its tendency to aggregation in biopolymer-based nanocomposites limits its application for food packaging and has been few studied.					
31726154	6	8	theme	acid	895:898	arg1	medium					900:905	acid medium	895:905	acid medium	895:905	TiO2 nanoparticles showed better dispersion in acid medium than water.					
31726154	10	9	theme	TiO2	1388:1391	arg1	aggregation					1393:1403	TiO2 aggregation	1388:1403	TiO2 aggregation	1388:1403	It was also concluded that TiO2 aggregation generated more biphasic regions in HPMC than generated in gelatin, which caused a microstructural reorganization in the matrices.					
31726154	5	10	theme	chemical	741:748	arg1	composition					750:760	chemical composition	741:760	chemical composition	741:760	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
31726154	8	11	from	aggregation	1185:1195	arg1	gelatin					1200:1206	gelatin	1200:1206	gelatin	1200:1206	The increase of relative opacity of the films as TiO2 was more attenuated for the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin.					
31726154	8	12	theme	due	1167:1169	arg1	films					1161:1165	the gelatin-TiO2 films	1144:1165	the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin	1144:1206	The increase of relative opacity of the films as TiO2 was more attenuated for the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin.					
31726154	5	13	theme	thermal	778:784	arg1	properties					801:810	thermal and mechanical properties	778:810	thermal and mechanical properties	778:810	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
31726154	8	14	theme	gelatin-TiO2	1148:1159	arg1	films					1161:1165	the gelatin-TiO2 films	1144:1165	the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin	1144:1206	The increase of relative opacity of the films as TiO2 was more attenuated for the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin.					
31726154	0	15	theme	methylcellulose-TiO2	14:33	arg1	films					66:70	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films	0:70	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.	0:114	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.					
31726154	6	16	theme	better	874:879	arg1	dispersion					881:890	better dispersion	874:890	better dispersion	874:890	TiO2 nanoparticles showed better dispersion in acid medium than water.					
31726154	0	17	theme	structural	93:102	arg1	properties					104:113	Physicochemical and structural properties	73:113	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.	0:114	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.					
31726154	1	18	theme	Photocatalytic	116:129	arg1	properties					131:140	Photocatalytic properties	116:140	Photocatalytic properties of titanium dioxide (TiO2)	116:167	Photocatalytic properties of titanium dioxide (TiO2) have been widely studied.					
31726154	9	19	theme	films	1287:1291	arg1	Morphology					1209:1218	Morphology	1209:1218	Morphology	1209:1218	Morphology, chemical composition, crystallinity and thermal properties of the films evidenced that TiO2 was better dispersed in both matrices at 1 wt%.					
31726154	9	19	theme	films	1287:1291	arg1	properties					1269:1278	thermal properties	1261:1278	thermal properties	1261:1278	Morphology, chemical composition, crystallinity and thermal properties of the films evidenced that TiO2 was better dispersed in both matrices at 1 wt%.					
31726154	9	19	theme	films	1287:1291	arg1	composition					1230:1240	chemical composition	1221:1240	chemical composition	1221:1240	Morphology, chemical composition, crystallinity and thermal properties of the films evidenced that TiO2 was better dispersed in both matrices at 1 wt%.					
31726154	9	19	theme	films	1287:1291	arg1	crystallinity					1243:1255	crystallinity	1243:1255	crystallinity	1243:1255	Morphology, chemical composition, crystallinity and thermal properties of the films evidenced that TiO2 was better dispersed in both matrices at 1 wt%.					
31726154	0	20	theme	Hydroxypropyl	0:12	arg1	methylcellulose-TiO2					14:33	Hydroxypropyl methylcellulose-TiO2	0:33	Hydroxypropyl methylcellulose-TiO2	0:33	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.					
31726154	4	21	theme	TiO2	551:554	arg1	nanoparticles					556:568	TiO2 nanoparticles	551:568	TiO2 nanoparticles	551:568	Particle size and zeta potential of TiO2 nanoparticles were investigated.					
31726154	5	22	theme	moisture	649:656	arg1	content					658:664	moisture content	649:664	moisture content	649:664	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
31726154	5	23	theme	Nanocomposite	589:601	arg1	films					603:607	Nanocomposite films	589:607	Nanocomposite films	589:607	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
31726154	3	24	theme	hydroxypropyl	422:434	arg1	methylcellulose					436:450	the hydroxypropyl methylcellulose	418:450	the hydroxypropyl methylcellulose (HPMC-TiO2)	418:462	The aim of this work was to study the dispersion of TiO2 (0-2 wt%) incorporated in the hydroxypropyl methylcellulose (HPMC-TiO2) and gelatin (gelatin-TiO2) film forming solutions.					
31726154	3	24	theme	hydroxypropyl	422:434	arg1	HPMC-TiO2					453:461	HPMC-TiO2	453:461	HPMC-TiO2	453:461	The aim of this work was to study the dispersion of TiO2 (0-2 wt%) incorporated in the hydroxypropyl methylcellulose (HPMC-TiO2) and gelatin (gelatin-TiO2) film forming solutions.					
31726154	0	25	theme	gelatin-TiO2	39:50	arg1	nanocomposite					52:64	gelatin-TiO2 nanocomposite	39:64	gelatin-TiO2 nanocomposite	39:64	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.					
31726154	7	26	theme	Moisture	919:926	arg1	content					928:934	Moisture content	919:934	Moisture content	919:934	Moisture content, water solubility and WVP of the gelatin-TiO2 films were influenced by the incorporation of TiO2, while HPMC-TiO2 films were not.					
31726154	10	27	theme	biphasic	1420:1427	arg1	regions					1429:1435	more biphasic regions	1415:1435	more biphasic regions in HPMC	1415:1443	It was also concluded that TiO2 aggregation generated more biphasic regions in HPMC than generated in gelatin, which caused a microstructural reorganization in the matrices.					
31726154	3	28	theme	TiO2	387:390	arg1	dispersion					373:382	the dispersion	369:382	the dispersion of TiO2 (0-2 wt%) incorporated in the hydroxypropyl methylcellulose (HPMC-TiO2) and gelatin (gelatin-TiO2) film forming solutions	369:512	The aim of this work was to study the dispersion of TiO2 (0-2 wt%) incorporated in the hydroxypropyl methylcellulose (HPMC-TiO2) and gelatin (gelatin-TiO2) film forming solutions.					
31726154	10	29	from	reorganization	1503:1516	arg1	matrices					1525:1532	the matrices	1521:1532	the matrices	1521:1532	It was also concluded that TiO2 aggregation generated more biphasic regions in HPMC than generated in gelatin, which caused a microstructural reorganization in the matrices.					
31726154	1	30	theme	titanium	145:152	arg1	TiO2					163:166	TiO2	163:166	TiO2	163:166	Photocatalytic properties of titanium dioxide (TiO2) have been widely studied.					
31726154	1	30	theme	titanium	145:152	arg1	dioxide					154:160	titanium dioxide	145:160	titanium dioxide (TiO2)	145:167	Photocatalytic properties of titanium dioxide (TiO2) have been widely studied.					
31726154	8	31	theme	TiO2	1180:1183	arg1	aggregation					1185:1195	lower TiO2 aggregation	1174:1195	lower TiO2 aggregation in gelatin	1174:1206	The increase of relative opacity of the films as TiO2 was more attenuated for the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin.					
31726154	4	32	theme	nanoparticles	556:568	arg1	size					524:527	Particle size	515:527	Particle size	515:527	Particle size and zeta potential of TiO2 nanoparticles were investigated.					
31726154	4	32	theme	nanoparticles	556:568	arg1	potential					538:546	zeta potential	533:546	zeta potential	533:546	Particle size and zeta potential of TiO2 nanoparticles were investigated.					
31726154	7	33	theme	HPMC-TiO2	1040:1048	arg1	films					1050:1054	HPMC-TiO2 films	1040:1054	HPMC-TiO2 films	1040:1054	Moisture content, water solubility and WVP of the gelatin-TiO2 films were influenced by the incorporation of TiO2, while HPMC-TiO2 films were not.					
31726154	0	34	theme	nanocomposite	52:64	arg1	films					66:70	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films	0:70	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.	0:114	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.					
31726154	10	35	from	regions	1429:1435	arg1	HPMC					1440:1443	HPMC	1440:1443	HPMC	1440:1443	It was also concluded that TiO2 aggregation generated more biphasic regions in HPMC than generated in gelatin, which caused a microstructural reorganization in the matrices.					
31726154	5	36	theme	relative	711:718	arg1	opacity					720:726	relative opacity	711:726	relative opacity	711:726	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
31726154	6	37	theme	TiO2	848:851	arg1	nanoparticles					853:865	TiO2 nanoparticles	848:865	TiO2 nanoparticles	848:865	TiO2 nanoparticles showed better dispersion in acid medium than water.					
31726154	5	38	theme	water	816:820	arg1	WVP					842:844	WVP	842:844	WVP	842:844	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
31726154	5	38	theme	water	816:820	arg1	permeability					828:839	water vapor permeability	816:839	water vapor permeability (WVP)	816:845	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
31726154	0	39	theme	Physicochemical	73:87	arg1	properties					104:113	Physicochemical and structural properties	73:113	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.	0:114	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.					
31726154	8	40	theme	opacity	1091:1097	arg1	increase					1070:1077	The increase	1066:1077	The increase of relative opacity of the films as TiO2	1066:1118	The increase of relative opacity of the films as TiO2 was more attenuated for the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin.					
31726154	0	41	dep	films	66:70	arg1	properties					104:113	Physicochemical and structural properties	73:113	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.	0:114	Hydroxypropyl methylcellulose-TiO2 and gelatin-TiO2 nanocomposite films: Physicochemical and structural properties.					
31726154	1	42	theme	dioxide	154:160	arg1	properties					131:140	Photocatalytic properties	116:140	Photocatalytic properties of titanium dioxide (TiO2)	116:167	Photocatalytic properties of titanium dioxide (TiO2) have been widely studied.					
31726154	5	43	theme	mechanical	790:799	arg1	properties					801:810	thermal and mechanical properties	778:810	thermal and mechanical properties	778:810	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
31726154	8	44	theme	relative	1082:1089	arg1	opacity					1091:1097	relative opacity	1082:1097	relative opacity of the films as TiO2	1082:1118	The increase of relative opacity of the films as TiO2 was more attenuated for the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin.					
31726154	9	45	theme	chemical	1221:1228	arg1	composition					1230:1240	chemical composition	1221:1240	chemical composition	1221:1240	Morphology, chemical composition, crystallinity and thermal properties of the films evidenced that TiO2 was better dispersed in both matrices at 1 wt%.					
31726154	2	46	theme	biopolymer-based	235:250	arg1	nanocomposites					252:265	biopolymer-based nanocomposites	235:265	biopolymer-based nanocomposites	235:265	However, its tendency to aggregation in biopolymer-based nanocomposites limits its application for food packaging and has been few studied.					
31726154	3	47	theme	film	491:494	arg1	solutions					504:512	film forming solutions	491:512	film forming solutions	491:512	The aim of this work was to study the dispersion of TiO2 (0-2 wt%) incorporated in the hydroxypropyl methylcellulose (HPMC-TiO2) and gelatin (gelatin-TiO2) film forming solutions.					
31726154	4	48	theme	zeta	533:536	arg1	potential					538:546	zeta potential	533:546	zeta potential	533:546	Particle size and zeta potential of TiO2 nanoparticles were investigated.					
31726154	7	49	theme	TiO2	1028:1031	arg1	incorporation					1011:1023	the incorporation	1007:1023	the incorporation of TiO2	1007:1031	Moisture content, water solubility and WVP of the gelatin-TiO2 films were influenced by the incorporation of TiO2, while HPMC-TiO2 films were not.					
31726154	9	50	from	%	1358:1358	arg1	matrices					1342:1349	both matrices	1337:1349	both matrices at 1 wt%	1337:1358	Morphology, chemical composition, crystallinity and thermal properties of the films evidenced that TiO2 was better dispersed in both matrices at 1 wt%.					
31726154	2	51	theme	food	294:297	arg1	packaging					299:307	food packaging	294:307	food packaging	294:307	However, its tendency to aggregation in biopolymer-based nanocomposites limits its application for food packaging and has been few studied.					
31726154	7	52	theme	films	982:986	arg1	WVP					958:960	WVP	958:960	WVP	958:960	Moisture content, water solubility and WVP of the gelatin-TiO2 films were influenced by the incorporation of TiO2, while HPMC-TiO2 films were not.					
31726154	7	52	theme	films	982:986	arg1	content					928:934	Moisture content	919:934	Moisture content	919:934	Moisture content, water solubility and WVP of the gelatin-TiO2 films were influenced by the incorporation of TiO2, while HPMC-TiO2 films were not.					
31726154	7	52	theme	films	982:986	arg1	solubility					943:952	water solubility	937:952	water solubility	937:952	Moisture content, water solubility and WVP of the gelatin-TiO2 films were influenced by the incorporation of TiO2, while HPMC-TiO2 films were not.					
31726154	8	53	theme	lower	1174:1178	arg1	aggregation					1185:1195	lower TiO2 aggregation	1174:1195	lower TiO2 aggregation in gelatin	1174:1206	The increase of relative opacity of the films as TiO2 was more attenuated for the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin.					
31726154	3	54	theme	work	351:354	arg1	aim					339:341	The aim	335:341	The aim of this work	335:354	The aim of this work was to study the dispersion of TiO2 (0-2 wt%) incorporated in the hydroxypropyl methylcellulose (HPMC-TiO2) and gelatin (gelatin-TiO2) film forming solutions.					
31726154	8	55	theme	films	1106:1110	arg1	opacity					1091:1097	relative opacity	1082:1097	relative opacity of the films as TiO2	1082:1118	The increase of relative opacity of the films as TiO2 was more attenuated for the gelatin-TiO2 films due to lower TiO2 aggregation in gelatin.					
31726154	5	56	theme	vapor	822:826	arg1	WVP					842:844	WVP	842:844	WVP	842:844	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
31726154	5	56	theme	vapor	822:826	arg1	permeability					828:839	water vapor permeability	816:839	water vapor permeability (WVP)	816:845	Nanocomposite films were characterized as to the thickness, moisture content, solubility, color, absorption to the light, relative opacity, morphology, chemical composition, crystallinity, thermal and mechanical properties and water vapor permeability (WVP).					
32228952	0	0	theme	tissue	111:116	arg1	applications					118:129	bone tissue applications	106:129	bone tissue applications	106:129	Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications.					
32228952	3	1	with	chitosan	613:620	arg1	GA					643:644	GA	643:644	GA	643:644	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	1	with	chitosan	613:620	arg1	glutaraldehyde					627:640	glutaraldehyde	627:640	glutaraldehyde (GA)	627:645	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	1	2	theme	alternative	177:187	arg1	strategy					189:196	an alternative strategy	174:196	an alternative strategy for bone grafting	174:214	The clinical demand for bone scaffolds as an alternative strategy for bone grafting has increased exponentially and, up to date, numerous formulations have been proposed to regenerate the bone tissue.					
32228952	3	3	theme	enhanced	843:850	arg1	response					863:870	enhanced biological response	843:870	enhanced biological response	843:870	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	1	4	theme	numerous	261:268	arg1	formulations					270:281	numerous formulations	261:281	numerous formulations	261:281	The clinical demand for bone scaffolds as an alternative strategy for bone grafting has increased exponentially and, up to date, numerous formulations have been proposed to regenerate the bone tissue.					
32228952	0	5	theme	bone	106:109	arg1	applications					118:129	bone tissue applications	106:129	bone tissue applications	106:129	Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications.					
32228952	3	6	theme	atomized	672:679	arg1	granules					706:713	different atomized calcium phosphates (CaP) granules	662:713	different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP)	662:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	2	7	dep	resistance	458:467	arg1	e.g.					441:444	e.g.	441:444	e.g.	441:444	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	7	8	theme	biphasic	1532:1539	arg1	ones					1541:1544	the biphasic ones	1528:1544	the biphasic ones	1528:1544	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	3	9	theme	biomechanical	816:828	arg1	behavior					830:837	improved biomechanical behavior	807:837	improved biomechanical behavior	807:837	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	5	10	theme	composite	1183:1191	arg1	scaffolds					1193:1201	the composite scaffolds	1179:1201	the composite scaffolds	1179:1201	The biological assessment of the composite scaffolds showed that the specimens with 0.2% crosslinking were the ones with the best biological performance.					
32228952	6	11	theme	granules	1343:1350	arg1	inclusion					1321:1329	the inclusion	1317:1329	the inclusion of biphasic granules	1317:1350	In addition, the inclusion of biphasic granules induced a trend for increase osteogenic activation, as compared to the addition of HA granules.					
32228952	3	12	theme	calcium	681:687	arg1	granules					706:713	different atomized calcium phosphates (CaP) granules	662:713	different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP)	662:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	2	13	dep	lack	367:370	arg1	etc					526:528	etc	526:528	etc	526:528	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	13	dep	lack	367:370	arg1	biodegradability					508:523	biodegradability	508:523	biodegradability	508:523	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	13	dep	lack	367:370	arg1	bioactivity					495:505	bioactivity	495:505	bioactivity	495:505	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	13	dep	lack	367:370	arg1	resistance					458:467	mechanical resistance	447:467	mechanical resistance	447:467	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	13	dep	lack	367:370	arg1	porosity					485:492	interconnected porosity	470:492	interconnected porosity	470:492	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	4	14	theme	CaP	1028:1030	arg1	granules					1032:1039	CaP granules	1028:1039	CaP granules	1028:1039	This innovative combination was designed to improve the scaffolds' functionality, in which GA improved chitosan mechanical strength and stability, whereas CaP granules enhanced the scaffolds' bioactivity and osteoblastic response, further reinforcing the scaffolds' structure.					
32228952	3	15	theme	tricalcium	772:781	arg1	phosphate					783:791	tricalcium phosphate	772:791	tricalcium phosphate (β-TCP)	772:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	15	theme	tricalcium	772:781	arg1	β-TCP					794:798	β-TCP	794:798	β-TCP	794:798	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	16	with	scaffolds	581:589	arg1	behavior					830:837	improved biomechanical behavior	807:837	improved biomechanical behavior	807:837	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	16	with	scaffolds	581:589	arg1	response					863:870	enhanced biological response	843:870	enhanced biological response	843:870	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	4	17	theme	mechanical	985:994	arg1	strength					996:1003	chitosan mechanical strength	976:1003	chitosan mechanical strength	976:1003	This innovative combination was designed to improve the scaffolds' functionality, in which GA improved chitosan mechanical strength and stability, whereas CaP granules enhanced the scaffolds' bioactivity and osteoblastic response, further reinforcing the scaffolds' structure.					
32228952	7	18	theme	HA	1513:1514	arg1	granules					1516:1523	HA granules	1513:1523	HA granules	1513:1523	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	5	19	theme	%	1237:1237	arg1	crosslinking					1239:1250	0.2% crosslinking	1234:1250	0.2% crosslinking	1234:1250	The biological assessment of the composite scaffolds showed that the specimens with 0.2% crosslinking were the ones with the best biological performance.					
32228952	2	20	theme	fundamental/ideal	392:408	arg1	properties					410:419	the fundamental/ideal properties	388:419	the fundamental/ideal properties of these materials	388:438	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	3	21	theme	CaP	701:703	arg1	granules					706:713	different atomized calcium phosphates (CaP) granules	662:713	different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP)	662:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	0	22	theme	Glutaraldehyde-crosslinking	0:26	arg1	chitosan					28:35	Glutaraldehyde-crosslinking chitosan	0:35	Glutaraldehyde-crosslinking chitosan	0:35	Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications.					
32228952	3	23	theme	biological	852:861	arg1	response					863:870	enhanced biological response	843:870	enhanced biological response	843:870	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	1	24	theme	bone	202:205	arg1	grafting					207:214	bone grafting	202:214	bone grafting	202:214	The clinical demand for bone scaffolds as an alternative strategy for bone grafting has increased exponentially and, up to date, numerous formulations have been proposed to regenerate the bone tissue.					
32228952	2	25	theme	mechanical	447:456	arg1	etc					526:528	etc	526:528	etc	526:528	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	25	theme	mechanical	447:456	arg1	biodegradability					508:523	biodegradability	508:523	biodegradability	508:523	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	25	theme	mechanical	447:456	arg1	bioactivity					495:505	bioactivity	495:505	bioactivity	495:505	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	25	theme	mechanical	447:456	arg1	resistance					458:467	mechanical resistance	447:467	mechanical resistance	447:467	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	25	theme	mechanical	447:456	arg1	porosity					485:492	interconnected porosity	470:492	interconnected porosity	470:492	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	5	26	with	ones	1261:1264	arg1	performance					1291:1301	the best biological performance	1271:1301	the best biological performance	1271:1301	The biological assessment of the composite scaffolds showed that the specimens with 0.2% crosslinking were the ones with the best biological performance.					
32228952	6	27	theme	HA	1435:1436	arg1	granules					1438:1445	HA granules	1435:1445	HA granules	1435:1445	In addition, the inclusion of biphasic granules induced a trend for increase osteogenic activation, as compared to the addition of HA granules.					
32228952	7	28	from	application	1681:1691	arg1	engineering					1708:1718	bone tissue engineering	1696:1718	bone tissue engineering	1696:1718	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	7	29	theme	biological	1626:1635	arg1	performance					1637:1647	enhanced biological performance	1617:1647	enhanced biological performance	1617:1647	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	6	30	theme	osteogenic	1381:1390	arg1	activation					1392:1401	osteogenic activation	1381:1401	osteogenic activation	1381:1401	In addition, the inclusion of biphasic granules induced a trend for increase osteogenic activation, as compared to the addition of HA granules.					
32228952	3	31	link	crosslinked	601:611	arg1	chitosan					613:620	crosslinked chitosan	601:620	crosslinked chitosan with glutaraldehyde (GA)	601:645	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	32	theme	innovative	560:569	arg1	scaffolds					581:589	innovative composite scaffolds	560:589	innovative composite scaffolds	560:589	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	5	33	theme	biological	1154:1163	arg1	assessment					1165:1174	The biological assessment	1150:1174	The biological assessment of the composite scaffolds	1150:1201	The biological assessment of the composite scaffolds showed that the specimens with 0.2% crosslinking were the ones with the best biological performance.					
32228952	5	34	theme	biological	1280:1289	arg1	performance					1291:1301	the best biological performance	1271:1301	the best biological performance	1271:1301	The biological assessment of the composite scaffolds showed that the specimens with 0.2% crosslinking were the ones with the best biological performance.					
32228952	2	35	theme	materials	430:438	arg1	properties					410:419	the fundamental/ideal properties	388:419	the fundamental/ideal properties of these materials	388:438	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	4	36	theme	innovative	878:887	arg1	combination					889:899	This innovative combination	873:899	This innovative combination	873:899	This innovative combination was designed to improve the scaffolds' functionality, in which GA improved chitosan mechanical strength and stability, whereas CaP granules enhanced the scaffolds' bioactivity and osteoblastic response, further reinforcing the scaffolds' structure.					
32228952	1	37	theme	clinical	136:143	arg1	demand					145:150	The clinical demand	132:150	The clinical demand for bone scaffolds as an alternative strategy for bone grafting	132:214	The clinical demand for bone scaffolds as an alternative strategy for bone grafting has increased exponentially and, up to date, numerous formulations have been proposed to regenerate the bone tissue.					
32228952	0	38	theme	calcium	63:69	arg1	granules					93:100	calcium phosphate spray-dried granules	63:100	calcium phosphate spray-dried granules for bone tissue applications	63:129	Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications.					
32228952	7	39	theme	GA	1578:1579	arg1	concentrations					1560:1573	low concentrations	1556:1573	low concentrations of GA	1556:1579	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	4	40	theme	osteoblastic	1081:1092	arg1	response					1094:1101	osteoblastic response	1081:1101	osteoblastic response	1081:1101	This innovative combination was designed to improve the scaffolds' functionality, in which GA improved chitosan mechanical strength and stability, whereas CaP granules enhanced the scaffolds' bioactivity and osteoblastic response, further reinforcing the scaffolds' structure.					
32228952	2	41	theme	interconnected	470:483	arg1	resistance					458:467	mechanical resistance	447:467	mechanical resistance	447:467	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	41	theme	interconnected	470:483	arg1	porosity					485:492	interconnected porosity	470:492	interconnected porosity	470:492	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	1	42	theme	bone	320:323	arg1	tissue					325:330	the bone tissue	316:330	the bone tissue	316:330	The clinical demand for bone scaffolds as an alternative strategy for bone grafting has increased exponentially and, up to date, numerous formulations have been proposed to regenerate the bone tissue.					
32228952	7	43	theme	adequate	1593:1600	arg1	properties					1602:1611	adequate properties	1593:1611	adequate properties	1593:1611	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	7	44	theme	present	1489:1495	arg1	work					1497:1500	the present work	1485:1500	the present work	1485:1500	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	2	45	theme	properties	410:419	arg1	one					381:383	one	381:383	one	381:383	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	2	45	theme	properties	410:419	arg1	properties					410:419	the fundamental/ideal properties	388:419	the fundamental/ideal properties of these materials	388:438	However, most of these structures lack at least one of the fundamental/ideal properties of these materials (e.g., mechanical resistance, interconnected porosity, bioactivity, biodegradability, etc.).					
32228952	0	46	theme	spray-dried	81:91	arg1	granules					93:100	calcium phosphate spray-dried granules	63:100	calcium phosphate spray-dried granules for bone tissue applications	63:129	Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications.					
32228952	7	47	theme	low	1556:1558	arg1	concentrations					1560:1573	low concentrations	1556:1573	low concentrations of GA	1556:1579	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	3	48	theme	composite	571:579	arg1	scaffolds					581:589	innovative composite scaffolds	560:589	innovative composite scaffolds	560:589	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	0	49	theme	phosphate	71:79	arg1	granules					93:100	calcium phosphate spray-dried granules	63:100	calcium phosphate spray-dried granules for bone tissue applications	63:129	Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications.					
32228952	3	50	dep	granules	706:713	arg1	HA					733:734	HA	733:734	HA	733:734	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	50	dep	granules	706:713	arg1	hydroxyapatite					717:730	hydroxyapatite	717:730	hydroxyapatite (HA)	717:735	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	50	dep	granules	706:713	arg1	mixtures					749:756	biphasic mixtures	740:756	biphasic mixtures of HA and β - tricalcium phosphate (β-TCP)	740:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	7	51	theme	bone	1696:1699	arg1	engineering					1708:1718	bone tissue engineering	1696:1718	bone tissue engineering	1696:1718	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	3	52	theme	biphasic	740:747	arg1	mixtures					749:756	biphasic mixtures	740:756	biphasic mixtures of HA and β - tricalcium phosphate (β-TCP)	740:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	53	theme	different	662:670	arg1	granules					706:713	different atomized calcium phosphates (CaP) granules	662:713	different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP)	662:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	5	54	theme	scaffolds	1193:1201	arg1	assessment					1165:1174	The biological assessment	1150:1174	The biological assessment of the composite scaffolds	1150:1201	The biological assessment of the composite scaffolds showed that the specimens with 0.2% crosslinking were the ones with the best biological performance.					
32228952	1	55	theme	bone	156:159	arg1	scaffolds					161:169	bone scaffolds	156:169	bone scaffolds as an alternative strategy for bone grafting	156:214	The clinical demand for bone scaffolds as an alternative strategy for bone grafting has increased exponentially and, up to date, numerous formulations have been proposed to regenerate the bone tissue.					
32228952	3	56	theme	improved	807:814	arg1	behavior					830:837	improved biomechanical behavior	807:837	improved biomechanical behavior	807:837	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	57	theme	β	768:768	arg1	HA					733:734	HA	733:734	HA	733:734	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	57	theme	β	768:768	arg1	hydroxyapatite					717:730	hydroxyapatite	717:730	hydroxyapatite (HA)	717:735	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	57	theme	β	768:768	arg1	mixtures					749:756	biphasic mixtures	740:756	biphasic mixtures of HA and β - tricalcium phosphate (β-TCP)	740:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	0	58	dep	scaffolds	37:45	arg1	reinforced					47:56	reinforced	47:56	scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications	37:129	Glutaraldehyde-crosslinking chitosan scaffolds reinforced with calcium phosphate spray-dried granules for bone tissue applications.					
32228952	5	59	with	specimens	1219:1227	arg1	crosslinking					1239:1250	0.2% crosslinking	1234:1250	0.2% crosslinking	1234:1250	The biological assessment of the composite scaffolds showed that the specimens with 0.2% crosslinking were the ones with the best biological performance.					
32228952	7	60	theme	enhanced	1617:1624	arg1	performance					1637:1647	enhanced biological performance	1617:1647	enhanced biological performance	1617:1647	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	7	61	theme	potential	1656:1664	arg1	candidates					1666:1675	potential candidates	1656:1675	potential candidates for application in bone tissue engineering	1656:1718	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	6	62	theme	granules	1438:1445	arg1	addition					1423:1430	the addition	1419:1430	the addition of HA granules	1419:1445	In addition, the inclusion of biphasic granules induced a trend for increase osteogenic activation, as compared to the addition of HA granules.					
32228952	7	63	theme	tissue	1701:1706	arg1	engineering					1708:1718	bone tissue engineering	1696:1718	bone tissue engineering	1696:1718	In conclusion, scaffolds produced in the present work, both with HA granules or the biphasic ones, and with low concentrations of GA, have shown adequate properties and enhanced biological performance, being potential candidates for application in bone tissue engineering.					
32228952	3	64	theme	HA	761:762	arg1	HA					733:734	HA	733:734	HA	733:734	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	64	theme	HA	761:762	arg1	hydroxyapatite					717:730	hydroxyapatite	717:730	hydroxyapatite (HA)	717:735	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	64	theme	HA	761:762	arg1	mixtures					749:756	biphasic mixtures	740:756	biphasic mixtures of HA and β - tricalcium phosphate (β-TCP)	740:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	4	65	theme	chitosan	976:983	arg1	strength					996:1003	chitosan mechanical strength	976:1003	chitosan mechanical strength	976:1003	This innovative combination was designed to improve the scaffolds' functionality, in which GA improved chitosan mechanical strength and stability, whereas CaP granules enhanced the scaffolds' bioactivity and osteoblastic response, further reinforcing the scaffolds' structure.					
32228952	3	66	theme	phosphates	689:698	arg1	granules					706:713	different atomized calcium phosphates (CaP) granules	662:713	different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP)	662:799	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	3	67	theme	crosslinked	601:611	arg1	chitosan					613:620	crosslinked chitosan	601:620	crosslinked chitosan with glutaraldehyde (GA)	601:645	In this work, we developed innovative composite scaffolds, based on crosslinked chitosan with glutaraldehyde (GA), combined with different atomized calcium phosphates (CaP) granules - hydroxyapatite (HA) or biphasic mixtures of HA and β - tricalcium phosphate (β-TCP), with improved biomechanical behavior and enhanced biological response.					
32228952	5	68	theme	0.2	1234:1236	arg1	%					1237:1237	%	1237:1237	%	1237:1237	The biological assessment of the composite scaffolds showed that the specimens with 0.2% crosslinking were the ones with the best biological performance.					
32228952	6	69	theme	biphasic	1334:1341	arg1	granules					1343:1350	biphasic granules	1334:1350	biphasic granules	1334:1350	In addition, the inclusion of biphasic granules induced a trend for increase osteogenic activation, as compared to the addition of HA granules.					
31128185	0	0	theme	cells	112:116	arg1	invasion					68:75	bacterial invasion	58:75	bacterial invasion	58:75	Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli.					
31128185	0	0	theme	cells	112:116	arg1	colonization					81:92	colonization	81:92	colonization of gut epithelial cells	81:116	Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli.					
31128185	1	1	theme	round	337:341	arg1	particles					350:358	round shaped particles	337:358	round shaped particles	337:358	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	5	2	theme	PAC-CHT	860:866	arg1	30:1					878:881	PAC-CHT NPs ratio 30:1	860:881	PAC-CHT NPs ratio 30:1	860:881	PAC-CHT NPs ratio 30:1 agglutinated ExPEC and decreased the ability of ExPEC to invade epithelial cells in a dose-dependent manner.					
31128185	0	3	theme	epithelial	101:110	arg1	cells					112:116	gut epithelial cells	97:116	gut epithelial cells	97:116	Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli.					
31128185	1	4	theme	shaped	343:348	arg1	particles					350:358	round shaped particles	337:358	round shaped particles	337:358	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	1	5	theme	PAC-CHT	228:234	arg1	nanoparticles					213:225	Cranberry proanthocyanidin-chitosan composite nanoparticles	167:225	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs)	167:239	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	1	5	theme	PAC-CHT	228:234	arg1	NPs					236:238	PAC-CHT NPs	228:238	PAC-CHT NPs	228:238	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	5	6	theme	ExPEC	931:935	arg1	ability					920:926	the ability	916:926	the ability of ExPEC to invade epithelial cells in a dose-dependent manner	916:989	PAC-CHT NPs ratio 30:1 agglutinated ExPEC and decreased the ability of ExPEC to invade epithelial cells in a dose-dependent manner.					
31128185	3	7	theme	coli	563:566	arg1	inhibition					580:589	inhibition	580:589	inhibition of gut epithelial cell invasion by ExPEC	580:630	PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC.					
31128185	3	7	theme	coli	563:566	arg1	agglutination					506:518	agglutination	506:518	agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC)	506:574	PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC.					
31128185	3	8	theme	NPs	474:476	arg1	bioactivity					478:488	PAC-CHT NPs bioactivity	466:488	PAC-CHT NPs bioactivity	466:488	PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC.					
31128185	6	9	theme	bacterial	1131:1139	arg1	infections					1141:1150	ExPEC bacterial infections	1125:1150	ExPEC bacterial infections	1125:1150	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	5	10	theme	NPs	868:870	arg1	30:1					878:881	PAC-CHT NPs ratio 30:1	860:881	PAC-CHT NPs ratio 30:1	860:881	PAC-CHT NPs ratio 30:1 agglutinated ExPEC and decreased the ability of ExPEC to invade epithelial cells in a dose-dependent manner.					
31128185	4	11	theme	618.7 nm	758:765	arg1	diameters					736:744	diameters	736:744	diameters of 122.8 to 618.7 nm	736:765	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	4	11	theme	618.7 nm	758:765	arg1	index					785:789	a polydispersity index	768:789	a polydispersity index of approximated 0.4 to 0.5	768:816	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	4	11	theme	618.7 nm	758:765	arg1	potential					830:838	a zeta potential	823:838	a zeta potential of 34.5 to 54.4 mV	823:857	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	6	12	theme	round-shaped	1036:1047	arg1	nanoparticles					1064:1076	stable, round-shaped, and bioactive nanoparticles	1028:1076	stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections	1028:1150	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	6	13	theme	stable	1028:1033	arg1	nanoparticles					1064:1076	stable, round-shaped, and bioactive nanoparticles	1028:1076	stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections	1028:1150	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	0	14	theme	composite	26:34	arg1	nanoparticles					36:48	Proanthocyanidin-chitosan composite nanoparticles	0:48	Proanthocyanidin-chitosan composite nanoparticles	0:48	Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli.					
31128185	4	15	dep	54.4 mV	851:857	arg1	to					848:849	to	848:849	to	848:849	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	4	16	theme	zeta	825:828	arg1	potential					830:838	a zeta potential	823:838	a zeta potential of 34.5 to 54.4 mV	823:857	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	4	17	dep	618.7 nm	758:765	arg1	to					755:756	to	755:756	to	755:756	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	0	18	theme	Proanthocyanidin-chitosan	0:24	arg1	nanoparticles					36:48	Proanthocyanidin-chitosan composite nanoparticles	0:48	Proanthocyanidin-chitosan composite nanoparticles	0:48	Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli.					
31128185	5	19	theme	dose-dependent	969:982	arg1	manner					984:989	a dose-dependent manner	967:989	a dose-dependent manner	967:989	PAC-CHT NPs ratio 30:1 agglutinated ExPEC and decreased the ability of ExPEC to invade epithelial cells in a dose-dependent manner.					
31128185	2	20	theme	surface	421:427	arg1	charge					429:434	surface charge	421:434	surface charge	421:434	The PAC-CHT NPs were characterized by size, polydispersity, surface charge, morphology, and PAC content.					
31128185	4	21	theme	ratio	684:688	arg1	10:1					690:693	CHT ratio 10:1	680:693	CHT ratio 10:1	680:693	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	4	22	theme	CHT	680:682	arg1	10:1					690:693	CHT ratio 10:1	680:693	CHT ratio 10:1	680:693	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	6	23	theme	potential	1082:1090	arg1	applications					1092:1103	potential applications	1082:1103	potential applications in the treatment of ExPEC bacterial infections	1082:1150	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	3	24	theme	gut	594:596	arg1	invasion					614:621	gut epithelial cell invasion	594:621	gut epithelial cell invasion	594:621	PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC.					
31128185	6	25	theme	ratio	1004:1008	arg1	10:1					1010:1013	PAC-CHT NPs ratio 10:1	992:1013	PAC-CHT NPs ratio 10:1 to 30:1	992:1021	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	0	26	theme	bacterial	58:66	arg1	invasion					68:75	bacterial invasion	58:75	bacterial invasion	58:75	Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli.					
31128185	1	27	theme	CHT	312:314	arg1	ratio					323:327	CHT weight ratio	312:327	CHT weight ratio	312:327	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	3	28	theme	epithelial	598:607	arg1	invasion					614:621	gut epithelial cell invasion	594:621	gut epithelial cell invasion	594:621	PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC.					
31128185	6	29	theme	NPs	1000:1002	arg1	10:1					1010:1013	PAC-CHT NPs ratio 10:1	992:1013	PAC-CHT NPs ratio 10:1 to 30:1	992:1021	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	2	30	theme	PAC	453:455	arg1	content					457:463	PAC content	453:463	PAC content	453:463	The PAC-CHT NPs were characterized by size, polydispersity, surface charge, morphology, and PAC content.					
31128185	1	31	theme	weight	316:321	arg1	ratio					323:327	CHT weight ratio	312:327	CHT weight ratio	312:327	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	4	32	theme	0.5	814:816	arg1	diameters					736:744	diameters	736:744	diameters of 122.8 to 618.7 nm	736:765	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	4	32	theme	0.5	814:816	arg1	index					785:789	a polydispersity index	768:789	a polydispersity index of approximated 0.4 to 0.5	768:816	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	4	32	theme	0.5	814:816	arg1	potential					830:838	a zeta potential	823:838	a zeta potential of 34.5 to 54.4 mV	823:857	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	6	33	theme	bioactive	1054:1062	arg1	nanoparticles					1064:1076	stable, round-shaped, and bioactive nanoparticles	1028:1076	stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections	1028:1150	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	5	34	theme	ratio	872:876	arg1	30:1					878:881	PAC-CHT NPs ratio 30:1	860:881	PAC-CHT NPs ratio 30:1	860:881	PAC-CHT NPs ratio 30:1 agglutinated ExPEC and decreased the ability of ExPEC to invade epithelial cells in a dose-dependent manner.					
31128185	6	35	theme	infections	1141:1150	arg1	treatment					1112:1120	the treatment	1108:1120	the treatment of ExPEC bacterial infections	1108:1150	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	4	36	theme	54.4 mV	851:857	arg1	diameters					736:744	diameters	736:744	diameters of 122.8 to 618.7 nm	736:765	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	4	36	theme	54.4 mV	851:857	arg1	index					785:789	a polydispersity index	768:789	a polydispersity index of approximated 0.4 to 0.5	768:816	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	4	36	theme	54.4 mV	851:857	arg1	potential					830:838	a zeta potential	823:838	a zeta potential of 34.5 to 54.4 mV	823:857	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	6	37	from	applications	1092:1103	arg1	treatment					1112:1120	the treatment	1108:1120	the treatment of ExPEC bacterial infections	1108:1150	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	4	38	theme	stable	710:715	arg1	nanoparticles					717:729	stable nanoparticles	710:729	stable nanoparticles	710:729	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	3	39	theme	invasion	614:621	arg1	inhibition					580:589	inhibition	580:589	inhibition of gut epithelial cell invasion by ExPEC	580:630	PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC.					
31128185	3	39	theme	invasion	614:621	arg1	agglutination					506:518	agglutination	506:518	agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC)	506:574	PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC.					
31128185	5	40	theme	epithelial	947:956	arg1	cells					958:962	epithelial cells	947:962	epithelial cells	947:962	PAC-CHT NPs ratio 30:1 agglutinated ExPEC and decreased the ability of ExPEC to invade epithelial cells in a dose-dependent manner.					
31128185	4	41	theme	polydispersity	770:783	arg1	index					785:789	a polydispersity index	768:789	a polydispersity index of approximated 0.4 to 0.5	768:816	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	1	42	theme	Cranberry	167:175	arg1	nanoparticles					213:225	Cranberry proanthocyanidin-chitosan composite nanoparticles	167:225	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs)	167:239	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	1	42	theme	Cranberry	167:175	arg1	NPs					236:238	PAC-CHT NPs	228:238	PAC-CHT NPs	228:238	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	6	43	theme	PAC-CHT	992:998	arg1	10:1					1010:1013	PAC-CHT NPs ratio 10:1	992:1013	PAC-CHT NPs ratio 10:1 to 30:1	992:1021	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	6	44	theme	ExPEC	1125:1129	arg1	infections					1141:1150	ExPEC bacterial infections	1125:1150	ExPEC bacterial infections	1125:1150	PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections.					
31128185	1	45	theme	proanthocyanidin-chitosan	177:201	arg1	nanoparticles					213:225	Cranberry proanthocyanidin-chitosan composite nanoparticles	167:225	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs)	167:239	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	1	45	theme	proanthocyanidin-chitosan	177:201	arg1	NPs					236:238	PAC-CHT NPs	228:238	PAC-CHT NPs	228:238	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	3	46	theme	PAC-CHT	466:472	arg1	bioactivity					478:488	PAC-CHT NPs bioactivity	466:488	PAC-CHT NPs bioactivity	466:488	PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC.					
31128185	0	47	theme	gut	97:99	arg1	cells					112:116	gut epithelial cells	97:116	gut epithelial cells	97:116	Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli.					
31128185	3	48	theme	cell	609:612	arg1	invasion					614:621	gut epithelial cell invasion	594:621	gut epithelial cell invasion	594:621	PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC.					
31128185	4	49	dep	0.5	814:816	arg1	to					811:812	to	811:812	to	811:812	Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV.					
31128185	1	50	theme	composite	203:211	arg1	nanoparticles					213:225	Cranberry proanthocyanidin-chitosan composite nanoparticles	167:225	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs)	167:239	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	1	50	theme	composite	203:211	arg1	NPs					236:238	PAC-CHT NPs	228:238	PAC-CHT NPs	228:238	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	1	51	dep	2:1	263:265	arg1	PAC					305:307	PAC	305:307	PAC	305:307	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
31128185	2	52	theme	PAC-CHT	365:371	arg1	NPs					373:375	The PAC-CHT NPs	361:375	The PAC-CHT NPs	361:375	The PAC-CHT NPs were characterized by size, polydispersity, surface charge, morphology, and PAC content.					
31128185	1	53	theme	15:1	279:282	arg1	20:1					284:287	15:1 20:1	279:287	15:1 20:1	279:287	Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles.					
29549767	6	0	theme	static	1401:1406	arg1	conditions					1417:1426	both static and flow conditions	1396:1426	conditions	1417:1426	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	2	1	theme	venous	772:777	arg1	flow					779:782	comparable arterial and venous flow	748:782	comparable arterial and venous flow	748:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	12	2	theme	blood	2279:2283	arg1	cells					2285:2289	red blood cells	2275:2289	red blood cells	2275:2289	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	2	3	dep	brushes	555:561	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	3	dep	brushes	555:561	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	3	dep	brushes	555:561	arg1	phosphorylcholine					631:647	2-methacryloyloxyethyl phosphorylcholine	608:647	2-methacryloyloxyethyl phosphorylcholine	608:647	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	3	dep	brushes	555:561	arg1	poly					565:568	poly	565:568	poly(N, N-dimethylacrylamide) (PDMA)	565:600	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	3	dep	brushes	555:561	arg1	PDMA					596:599	PDMA	596:599	PDMA	596:599	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	3	dep	brushes	555:561	arg1	N					570:570	N	570:570	N	570:570	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	3	dep	brushes	555:561	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	3	dep	brushes	555:561	arg1	PMPC					651:654	PMPC	651:654	PMPC	651:654	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	12	4	from	blood	2238:2242	arg1	adhesion					2193:2200	platelet adhesion	2184:2200	platelet adhesion from platelet rich plasma and whole blood	2184:2242	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	1	5	theme	corona	183:188	arg1	changes					199:205	its changes	195:205	its changes	195:205	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	1	5	theme	corona	183:188	arg1	nature					165:170	the nature	161:170	the nature of protein corona	161:188	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	1	5	theme	corona	183:188	arg1	binding					217:223	cell binding	212:223	cell binding	212:223	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	3	6	theme	substrates	981:990	arg1	placement					964:972	the placement	960:972	the placement of the substrates within the blood loop	960:1012	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	8	7	theme	coagulation	1649:1659	arg1	fibronectin					1708:1718	fibronectin	1708:1718	fibronectin	1708:1718	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	8	7	theme	coagulation	1649:1659	arg1	vitronectin					1692:1702	vitronectin	1692:1702	vitronectin	1692:1702	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	8	7	theme	coagulation	1649:1659	arg1	proteins					1661:1668	coagulation proteins	1649:1668	coagulation proteins	1649:1668	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	8	7	theme	coagulation	1649:1659	arg1	proteins					1682:1689	complement proteins	1671:1689	complement proteins	1671:1689	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	7	8	theme	brushes	1569:1575	arg1	chemistry					1544:1552	the chemistry	1540:1552	the chemistry of the polymer brushes	1540:1575	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	2	9	from	conditions	796:805	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	9	from	conditions	796:805	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	9	from	conditions	796:805	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	9	from	conditions	796:805	arg1	poly					565:568	poly	565:568	poly(N, N-dimethylacrylamide) (PDMA)	565:600	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	9	from	conditions	796:805	arg1	PHPMA					703:707	PHPMA	703:707	PHPMA	703:707	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	10	theme	loop	734:737	arg1	model					739:743	an in vitro blood loop model	716:743	an in vitro blood loop model	716:743	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	9	11	theme	static	1891:1896	arg1	conditions					1898:1907	both flow and static conditions	1877:1907	conditions	1898:1907	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	11	12	theme	red	2126:2128	arg1	cell					2136:2139	red blood cell	2126:2139	red blood cell	2126:2139	The nature of the protein corona formed in flow conditions influenced the platelet and red blood cell binding.					
29549767	2	13	theme	antifouling	535:545	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	13	theme	antifouling	535:545	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	13	theme	antifouling	535:545	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	13	theme	antifouling	535:545	arg1	poly					565:568	poly	565:568	poly(N, N-dimethylacrylamide) (PDMA)	565:600	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	13	theme	antifouling	535:545	arg1	PHPMA					703:707	PHPMA	703:707	PHPMA	703:707	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	8	14	theme	flow	1610:1613	arg1	conditions					1615:1624	flow conditions	1610:1624	flow conditions	1610:1624	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	4	15	theme	brush	1042:1046	arg1	substrates					1057:1066	the brush modified substrates	1038:1066	the brush modified substrates	1038:1066	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	6	16	theme	other	1378:1382	arg1	surfaces					1384:1391	other surfaces	1378:1391	other surfaces	1378:1391	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	12	17	theme	platelet	2207:2214	arg1	plasma					2221:2226	platelet rich plasma	2207:2226	platelet rich plasma	2207:2226	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	2	18	from	blood	501:505	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	18	from	blood	501:505	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	18	from	blood	501:505	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	18	from	blood	501:505	arg1	poly					565:568	poly	565:568	poly(N, N-dimethylacrylamide) (PDMA)	565:600	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	18	from	blood	501:505	arg1	PHPMA					703:707	PHPMA	703:707	PHPMA	703:707	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	1	19	from	changes	199:205	arg1	surface					240:246	biomaterial surface	228:246	biomaterial surface	228:246	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	7	20	dep	conditions	1506:1515	arg1	flow					1529:1532	flow	1529:1532	flow	1529:1532	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	7	20	dep	conditions	1506:1515	arg1	static					1518:1523	static	1518:1523	static	1518:1523	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	3	21	contain	containing	921:930	arg2	substrates					936:945	the substrates	932:945	the substrates to arrive at the placement of the substrates within the blood loop	932:1012	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	3	21	contain	containing	921:930	arg1	channel					913:919	a vertical channel	902:919	a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop	902:1012	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	10	22	theme	protein	1997:2003	arg1	corona					2005:2010	protein corona	1997:2010	protein corona	1997:2010	Unlike PDMA brush, the flow conditions did not affect the composition of protein corona on PMPC and PHPMA brushes.					
29549767	5	23	from	blood	1278:1282	arg1	surface					1265:1271	brush coated surface	1252:1271	brush coated surface from blood	1252:1282	The flow elevated fouling on brush coated surface from blood.					
29549767	6	24	theme	adhesion	1338:1345	arg1	lower					1367:1371	lower	1367:1371	lower	1367:1371	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	6	24	theme	adhesion	1338:1345	arg1	extent					1289:1294	The extent	1285:1294	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush	1285:1361	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	1	25	theme	thrombosis	329:338	arg1	mechanism					300:308	the mechanism	296:308	the mechanism of surface-induced thrombosis	296:338	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	11	26	theme	corona	2065:2070	arg1	nature					2043:2048	The nature	2039:2048	The nature of the protein corona formed in flow conditions	2039:2096	The nature of the protein corona formed in flow conditions influenced the platelet and red blood cell binding.					
29549767	11	27	theme	platelet	2113:2120	arg1	binding					2141:2147	the platelet and red blood cell binding	2109:2147	the platelet and red blood cell binding	2109:2147	The nature of the protein corona formed in flow conditions influenced the platelet and red blood cell binding.					
29549767	8	28	with	enriched	1635:1642	arg1	fibronectin					1708:1718	fibronectin	1708:1718	fibronectin	1708:1718	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	8	28	with	enriched	1635:1642	arg1	vitronectin					1692:1702	vitronectin	1692:1702	vitronectin	1692:1702	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	8	28	with	enriched	1635:1642	arg1	proteins					1661:1668	coagulation proteins	1649:1668	coagulation proteins	1649:1668	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	8	28	with	enriched	1635:1642	arg1	proteins					1682:1689	complement proteins	1671:1689	complement proteins	1671:1689	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	4	29	theme	fluorescence	1103:1114	arg1	microscopy					1116:1125	fluorescence microscopy	1103:1125	fluorescence microscopy	1103:1125	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	1	30	from	nature	165:170	arg1	surface					240:246	biomaterial surface	228:246	biomaterial surface	228:246	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	3	31	theme	fluid	810:814	arg1	dynamics					816:823	fluid dynamics	810:823	A fluid dynamics model	808:829	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	0	32	from	Influence	0:8	arg1	generation					100:109	surface-induced thrombus generation	75:109	surface-induced thrombus generation	75:109	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	0	32	from	Influence	0:8	arg1	corona					64:69	adsorbed plasma protein corona	40:69	adsorbed plasma protein corona	40:69	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	0	32	from	Influence	0:8	arg1	antifouling					114:124	antifouling	114:124	antifouling	114:124	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	2	33	theme	2-methacryloyloxyethyl	608:629	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	33	theme	2-methacryloyloxyethyl	608:629	arg1	phosphorylcholine					631:647	2-methacryloyloxyethyl phosphorylcholine	608:647	2-methacryloyloxyethyl phosphorylcholine	608:647	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	0	34	theme	thrombus	91:98	arg1	generation					100:109	surface-induced thrombus generation	75:109	surface-induced thrombus generation	75:109	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	10	35	theme	corona	2005:2010	arg1	composition					1982:1992	the composition	1978:1992	the composition of protein corona on PMPC and PHPMA brushes	1978:2036	Unlike PDMA brush, the flow conditions did not affect the composition of protein corona on PMPC and PHPMA brushes.					
29549767	8	36	theme	serum	1747:1751	arg1	albumin					1753:1759	serum albumin	1747:1759	serum albumin	1747:1759	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	7	37	theme	test	1501:1504	arg1	conditions					1506:1515	the test conditions	1497:1515	the test conditions (static vs. flow)	1497:1533	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	11	38	theme	cell	2136:2139	arg1	binding					2141:2147	the platelet and red blood cell binding	2109:2147	the platelet and red blood cell binding	2109:2147	The nature of the protein corona formed in flow conditions influenced the platelet and red blood cell binding.					
29549767	4	39	theme	mass	1187:1190	arg1	proteomics					1211:1220	mass spectrometry based proteomics	1187:1220	mass spectrometry based proteomics	1187:1220	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	2	40	theme	protein	391:397	arg1	corona					399:404	protein corona	391:404	protein corona	391:404	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	12	41	from	dependence	2154:2163	arg1	adhesion					2193:2200	platelet adhesion	2184:2200	platelet adhesion from platelet rich plasma and whole blood	2184:2242	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	7	42	from	dependence	1483:1492	arg1	conditions					1506:1515	the test conditions	1497:1515	the test conditions (static vs. flow)	1497:1533	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	2	43	theme	recalcified	444:454	arg1	plasma					462:467	heparinized recalcified human plasma	432:467	heparinized recalcified human plasma	432:467	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	12	44	theme	shear	2168:2172	arg1	stress					2174:2179	shear stress	2168:2179	shear stress	2168:2179	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	2	45	theme	whole	495:499	arg1	blood					501:505	whole blood	495:505	whole blood	495:505	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	3	46	theme	resulting	875:883	arg1	patterns					890:897	the resulting flow patterns	871:897	the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop	871:1012	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	11	47	theme	protein	2057:2063	arg1	corona					2065:2070	the protein corona	2053:2070	the protein corona formed in flow conditions	2053:2096	The nature of the protein corona formed in flow conditions influenced the platelet and red blood cell binding.					
29549767	4	48	theme	corona	1157:1162	arg1	microscopy					1116:1125	fluorescence microscopy	1103:1125	fluorescence microscopy	1103:1125	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	4	48	theme	corona	1157:1162	arg1	nature					1135:1140	the nature	1131:1140	the nature of the protein corona	1131:1162	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	5	49	theme	coated	1258:1263	arg1	surface					1265:1271	brush coated surface	1252:1271	brush coated surface from blood	1252:1282	The flow elevated fouling on brush coated surface from blood.					
29549767	2	50	theme	cell	416:419	arg1	binding					421:427	blood cell binding	410:427	blood cell binding	410:427	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	0	51	theme	dynamic	13:19	arg1	conditions					26:35	dynamic flow conditions	13:35	dynamic flow conditions	13:35	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	2	52	theme	platelet	470:477	arg1	plasma					484:489	platelet rich plasma	470:489	platelet rich plasma	470:489	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	12	53	theme	shear	2348:2352	arg1	condition					2354:2362	high shear condition	2343:2362	high shear condition	2343:2362	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	0	54	theme	conditions	26:35	arg1	Influence					0:8	Influence	0:8	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling	0:124	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	12	55	from	plasma	2221:2226	arg1	adhesion					2193:2200	platelet adhesion	2184:2200	platelet adhesion from platelet rich plasma and whole blood	2184:2242	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	2	56	from	nature	381:386	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	56	from	nature	381:386	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	56	from	nature	381:386	arg1	conditions					796:805	static conditions	789:805	static conditions	789:805	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	56	from	nature	381:386	arg1	poly					565:568	poly	565:568	poly(N, N-dimethylacrylamide) (PDMA)	565:600	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	56	from	nature	381:386	arg1	plasma					484:489	platelet rich plasma	470:489	platelet rich plasma	470:489	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	56	from	nature	381:386	arg1	PHPMA					703:707	PHPMA	703:707	PHPMA	703:707	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	56	from	nature	381:386	arg1	plasma					462:467	heparinized recalcified human plasma	432:467	heparinized recalcified human plasma	432:467	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	56	from	nature	381:386	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	9	57	theme	Apolipoprotein	1762:1775	arg1	proteins					1798:1805	complement proteins	1787:1805	complement proteins	1787:1805	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	9	57	theme	Apolipoprotein	1762:1775	arg1	proteins					1830:1837	the most abundant proteins	1812:1837	the most abundant proteins seen on PMPC and PHPMA surfaces	1812:1869	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	9	57	theme	Apolipoprotein	1762:1775	arg1	B-100					1777:1781	Apolipoprotein B-100	1762:1781	Apolipoprotein B-100	1762:1781	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	0	58	theme	protein	56:62	arg1	corona					64:69	adsorbed plasma protein corona	40:69	adsorbed plasma protein corona	40:69	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	0	59	theme	adsorbed	40:47	arg1	corona					64:69	adsorbed plasma protein corona	40:69	adsorbed plasma protein corona	40:69	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	7	60	theme	protein	1454:1460	arg1	corona					1462:1467	adsorbed protein corona	1445:1467	adsorbed protein corona	1445:1467	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	4	61	theme	based	1205:1209	arg1	proteomics					1211:1220	mass spectrometry based proteomics	1187:1220	mass spectrometry based proteomics	1187:1220	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	1	62	theme	cell	212:215	arg1	binding					217:223	cell binding	212:223	cell binding	212:223	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	2	63	theme	comparable	748:757	arg1	flow					779:782	comparable arterial and venous flow	748:782	comparable arterial and venous flow	748:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	10	64	theme	PHPMA	2024:2028	arg1	brushes					2030:2036	PHPMA brushes	2024:2036	PHPMA brushes	2024:2036	Unlike PDMA brush, the flow conditions did not affect the composition of protein corona on PMPC and PHPMA brushes.					
29549767	11	65	theme	flow	2082:2085	arg1	conditions					2087:2096	flow conditions	2082:2096	flow conditions	2082:2096	The nature of the protein corona formed in flow conditions influenced the platelet and red blood cell binding.					
29549767	10	66	theme	PDMA	1931:1934	arg1	brush					1936:1940	PDMA brush	1931:1940	PDMA brush	1931:1940	Unlike PDMA brush, the flow conditions did not affect the composition of protein corona on PMPC and PHPMA brushes.					
29549767	2	67	theme	static	789:794	arg1	conditions					796:805	static conditions	789:805	static conditions	789:805	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	8	68	with	enriched	1733:1740	arg1	albumin					1753:1759	serum albumin	1747:1759	serum albumin	1747:1759	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	6	69	theme	platelet	1329:1336	arg1	adhesion					1338:1345	platelet adhesion	1329:1345	platelet adhesion	1329:1345	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	8	70	theme	PDMA	1593:1596	arg1	brush					1598:1602	the PDMA brush	1589:1602	the PDMA brush under flow conditions	1589:1624	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	8	70	theme	PDMA	1593:1596	arg1	enriched					1635:1642	enriched	1635:1642	enriched	1635:1642	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	8	70	theme	PDMA	1593:1596	arg1	enriched					1733:1740	enriched	1733:1740	enriched	1733:1740	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	9	71	theme	abundant	1821:1828	arg1	proteins					1798:1805	complement proteins	1787:1805	complement proteins	1787:1805	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	9	71	theme	abundant	1821:1828	arg1	B-100					1777:1781	Apolipoprotein B-100	1762:1781	Apolipoprotein B-100	1762:1781	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	9	71	theme	abundant	1821:1828	arg1	proteins					1830:1837	the most abundant proteins	1812:1837	the most abundant proteins seen on PMPC and PHPMA surfaces	1812:1869	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	1	72	theme	dynamic	254:260	arg1	conditions					262:271	dynamic conditions	254:271	dynamic conditions	254:271	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	12	73	theme	red	2275:2277	arg1	cells					2285:2289	red blood cells	2275:2289	red blood cells	2275:2289	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	1	74	theme	protein	175:181	arg1	corona					183:188	protein corona	175:188	protein corona	175:188	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	6	75	theme	PDMA	1352:1355	arg1	brush					1357:1361	PDMA brush	1352:1361	PDMA brush	1352:1361	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	9	76	theme	PMPC	1847:1850	arg1	surfaces					1862:1869	PMPC and PHPMA surfaces	1847:1869	PMPC and PHPMA surfaces	1847:1869	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	7	77	theme	polymer	1561:1567	arg1	brushes					1569:1575	the polymer brushes	1557:1575	the polymer brushes	1557:1575	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	9	78	theme	PHPMA	1856:1860	arg1	surfaces					1862:1869	PMPC and PHPMA surfaces	1847:1869	PMPC and PHPMA surfaces	1847:1869	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	2	79	theme	polymer	547:553	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	79	theme	polymer	547:553	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	79	theme	polymer	547:553	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	79	theme	polymer	547:553	arg1	poly					565:568	poly	565:568	poly(N, N-dimethylacrylamide) (PDMA)	565:600	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	79	theme	polymer	547:553	arg1	PHPMA					703:707	PHPMA	703:707	PHPMA	703:707	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	80	from	plasma	484:489	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	80	from	plasma	484:489	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	80	from	plasma	484:489	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	80	from	plasma	484:489	arg1	poly					565:568	poly	565:568	poly(N, N-dimethylacrylamide) (PDMA)	565:600	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	80	from	plasma	484:489	arg1	PHPMA					703:707	PHPMA	703:707	PHPMA	703:707	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	4	81	theme	modified	1048:1055	arg1	substrates					1057:1066	the brush modified substrates	1038:1066	the brush modified substrates	1038:1066	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	2	82	dep	N	570:570	arg1	N-dimethylacrylamide					573:592	N-dimethylacrylamide	573:592	N-dimethylacrylamide	573:592	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	83	theme	hydrophilic	523:533	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	83	theme	hydrophilic	523:533	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	83	theme	hydrophilic	523:533	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	83	theme	hydrophilic	523:533	arg1	poly					565:568	poly	565:568	poly(N, N-dimethylacrylamide) (PDMA)	565:600	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	83	theme	hydrophilic	523:533	arg1	PHPMA					703:707	PHPMA	703:707	PHPMA	703:707	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	84	theme	2-hydroxypropyl	669:683	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	84	theme	2-hydroxypropyl	669:683	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	8	85	theme	complement	1671:1680	arg1	proteins					1661:1668	coagulation proteins	1649:1668	coagulation proteins	1649:1668	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	8	85	theme	complement	1671:1680	arg1	proteins					1682:1689	complement proteins	1671:1689	complement proteins	1671:1689	Specially, the PDMA brush under flow conditions was more enriched with coagulation proteins, complement proteins, vitronectin and fibronectin but was less enriched with serum albumin.					
29549767	4	86	from	binding	1027:1033	arg1	substrates					1057:1066	the brush modified substrates	1038:1066	the brush modified substrates	1038:1066	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	12	87	theme	rich	2216:2219	arg1	plasma					2221:2226	platelet rich plasma	2207:2226	platelet rich plasma	2207:2226	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	2	88	theme	poly[N-	661:667	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	88	theme	poly[N-	661:667	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	3	89	used	used	835:838	arg2	model					825:829	A fluid dynamics model	808:829	A fluid dynamics model	808:829	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	3	90	theme	dynamics	816:823	arg1	model					825:829	A fluid dynamics model	808:829	A fluid dynamics model	808:829	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	9	91	theme	complement	1787:1796	arg1	proteins					1798:1805	complement proteins	1787:1805	complement proteins	1787:1805	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	9	91	theme	complement	1787:1796	arg1	B-100					1777:1781	Apolipoprotein B-100	1762:1781	Apolipoprotein B-100	1762:1781	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	9	91	theme	complement	1787:1796	arg1	proteins					1830:1837	the most abundant proteins	1812:1837	the most abundant proteins seen on PMPC and PHPMA surfaces	1812:1869	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	11	92	theme	blood	2130:2134	arg1	cell					2136:2139	red blood cell	2126:2139	red blood cell	2126:2139	The nature of the protein corona formed in flow conditions influenced the platelet and red blood cell binding.					
29549767	12	93	theme	platelet	2184:2191	arg1	adhesion					2193:2200	platelet adhesion	2184:2200	platelet adhesion from platelet rich plasma and whole blood	2184:2242	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	6	94	theme	protein	1306:1312	arg1	adsorption					1314:1323	plasma protein adsorption	1299:1323	plasma protein adsorption	1299:1323	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	0	95	theme	surface-induced	75:89	arg1	generation					100:109	surface-induced thrombus generation	75:109	surface-induced thrombus generation	75:109	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	3	96	theme	blood	1003:1007	arg1	loop					1009:1012	the blood loop	999:1012	the blood loop	999:1012	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	12	97	from	adhesion	2313:2320	arg1	surface					2329:2335	the surface	2325:2335	the surface	2325:2335	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	2	98	theme	rich	479:482	arg1	plasma					484:489	platelet rich plasma	470:489	platelet rich plasma	470:489	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	6	99	theme	adsorption	1314:1323	arg1	lower					1367:1371	lower	1367:1371	lower	1367:1371	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	6	99	theme	adsorption	1314:1323	arg1	extent					1289:1294	The extent	1285:1294	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush	1285:1361	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	1	100	from	binding	217:223	arg1	surface					240:246	biomaterial surface	228:246	biomaterial surface	228:246	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	2	101	from	plasma	462:467	arg1	methacrylamide					686:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	poly[N-(2-hydroxypropyl) methacrylamide	661:699	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	101	from	plasma	462:467	arg1	poly					603:606	poly	603:606	poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC)	603:655	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	101	from	plasma	462:467	arg1	brushes					555:561	three highly hydrophilic antifouling polymer brushes	510:561	three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow	510:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	101	from	plasma	462:467	arg1	poly					565:568	poly	565:568	poly(N, N-dimethylacrylamide) (PDMA)	565:600	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	101	from	plasma	462:467	arg1	PHPMA					703:707	PHPMA	703:707	PHPMA	703:707	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	4	102	theme	spectrometry	1192:1203	arg1	proteomics					1211:1220	mass spectrometry based proteomics	1187:1220	mass spectrometry based proteomics	1187:1220	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	2	103	theme	blood	728:732	arg1	model					739:743	an in vitro blood loop model	716:743	an in vitro blood loop model	716:743	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	10	104	theme	flow	1947:1950	arg1	conditions					1952:1961	the flow conditions	1943:1961	the flow conditions	1943:1961	Unlike PDMA brush, the flow conditions did not affect the composition of protein corona on PMPC and PHPMA brushes.					
29549767	6	105	theme	plasma	1299:1304	arg1	adsorption					1314:1323	plasma protein adsorption	1299:1323	plasma protein adsorption	1299:1323	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	12	106	theme	stress	2174:2179	arg1	dependence					2154:2163	The dependence	2150:2163	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood	2150:2242	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	4	107	theme	protein	1149:1155	arg1	corona					1157:1162	the protein corona	1145:1162	the protein corona	1145:1162	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	10	108	from	composition	1982:1992	arg1	brushes					2030:2036	PHPMA brushes	2024:2036	PHPMA brushes	2024:2036	Unlike PDMA brush, the flow conditions did not affect the composition of protein corona on PMPC and PHPMA brushes.					
29549767	10	108	from	composition	1982:1992	arg1	PMPC					2015:2018	PMPC	2015:2018	PMPC	2015:2018	Unlike PDMA brush, the flow conditions did not affect the composition of protein corona on PMPC and PHPMA brushes.					
29549767	2	109	theme	heparinized	432:442	arg1	plasma					462:467	heparinized recalcified human plasma	432:467	heparinized recalcified human plasma	432:467	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	12	110	theme	high	2343:2346	arg1	condition					2354:2362	high shear condition	2343:2362	high shear condition	2343:2362	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	5	111	theme	brush	1252:1256	arg1	surface					1265:1271	brush coated surface	1252:1271	brush coated surface from blood	1252:1282	The flow elevated fouling on brush coated surface from blood.					
29549767	12	112	theme	whole	2232:2236	arg1	blood					2238:2242	whole blood	2232:2242	whole blood	2232:2242	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	0	113	theme	flow	21:24	arg1	conditions					26:35	dynamic flow conditions	13:35	dynamic flow conditions	13:35	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	12	114	theme	cells	2285:2289	arg1	contribution					2259:2270	the contribution	2255:2270	the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition	2255:2362	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	3	115	theme	flow	885:888	arg1	patterns					890:897	the resulting flow patterns	871:897	the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop	871:1012	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	3	116	from	patterns	890:897	arg1	channel					913:919	a vertical channel	902:919	a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop	902:1012	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	2	117	theme	binding	421:427	arg1	nature					381:386	the nature	377:386	the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions	377:805	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	118	theme	in vitro	719:726	arg1	model					739:743	an in vitro blood loop model	716:743	an in vitro blood loop model	716:743	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	2	119	theme	blood	410:414	arg1	binding					421:427	blood cell binding	410:427	blood cell binding	410:427	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	12	120	theme	platelet	2304:2311	arg1	adhesion					2313:2320	platelet adhesion	2304:2320	platelet adhesion on the surface	2304:2335	The dependence of shear stress on platelet adhesion from platelet rich plasma and whole blood highlights the contribution of red blood cells in enhancing platelet adhesion on the surface under high shear condition.					
29549767	2	121	theme	corona	399:404	arg1	nature					381:386	the nature	377:386	the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions	377:805	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	0	122	theme	plasma	49:54	arg1	corona					64:69	adsorbed plasma protein corona	40:69	adsorbed plasma protein corona	40:69	Influence of dynamic flow conditions on adsorbed plasma protein corona and surface-induced thrombus generation on antifouling brushes.					
29549767	7	123	theme	strong	1476:1481	arg1	dependence					1483:1492	strong dependence	1476:1492	strong dependence on the test conditions (static vs. flow)	1476:1533	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	3	124	theme	vertical	904:911	arg1	channel					913:919	a vertical channel	902:919	a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop	902:1012	A fluid dynamics model was used initially to better understand the resulting flow patterns in a vertical channel containing the substrates to arrive at the placement of the substrates within the blood loop.					
29549767	2	125	theme	human	456:460	arg1	plasma					462:467	heparinized recalcified human plasma	432:467	heparinized recalcified human plasma	432:467	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	1	126	theme	surface-induced	313:327	arg1	thrombosis					329:338	surface-induced thrombosis	313:338	surface-induced thrombosis	313:338	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
29549767	7	127	theme	corona	1462:1467	arg1	profiles					1433:1440	The profiles	1429:1440	The profiles of adsorbed protein corona	1429:1467	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	2	128	theme	arterial	759:766	arg1	flow					779:782	comparable arterial and venous flow	748:782	comparable arterial and venous flow	748:782	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	9	129	theme	flow	1882:1885	arg1	conditions					1898:1907	both flow and static conditions	1877:1907	conditions	1898:1907	Apolipoprotein B-100 and complement proteins were the most abundant proteins seen on PMPC and PHPMA surfaces under both flow and static conditions, respectively.					
29549767	7	130	theme	adsorbed	1445:1452	arg1	corona					1462:1467	adsorbed protein corona	1445:1467	adsorbed protein corona	1445:1467	The profiles of adsorbed protein corona showed strong dependence on the test conditions (static vs. flow), and the chemistry of the polymer brushes.					
29549767	4	131	theme	protein	1019:1025	arg1	binding					1027:1033	The protein binding	1015:1033	The protein binding on the brush modified substrates	1015:1066	The protein binding on the brush modified substrates was determined using ellipsometry, fluorescence microscopy and the nature of the protein corona was investigated using mass spectrometry based proteomics.					
29549767	2	132	from	brushes	555:561	arg1	nature					381:386	the nature	377:386	the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions	377:805	In this manuscript, we investigated the nature of protein corona and blood cell binding in heparinized recalcified human plasma, platelet rich plasma and whole blood on three highly hydrophilic antifouling polymer brushes, (poly(N, N-dimethylacrylamide) (PDMA), poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) and poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA) using an in vitro blood loop model at comparable arterial and venous flow, and static conditions.					
29549767	6	133	theme	flow	1412:1415	arg1	conditions					1417:1426	both static and flow conditions	1396:1426	conditions	1417:1426	The extent of plasma protein adsorption and platelet adhesion onto PDMA brush was lower than other surfaces in both static and flow conditions.					
29549767	1	134	theme	biomaterial	228:238	arg1	surface					240:246	biomaterial surface	228:246	biomaterial surface	228:246	The information regarding the nature of protein corona (and its changes) and cell binding on biomaterial surface under dynamic conditions is critical to dissect the mechanism of surface-induced thrombosis.					
30543027	9	0	from	potentials	1572:1581	arg1	patients					1630:1637	periodontitis patients	1616:1637	periodontitis patients	1616:1637	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	6	1	theme	THP-1-derived	1166:1178	arg1	macrophages					1180:1190	THP-1-derived macrophages	1166:1190	THP-1-derived macrophages	1166:1190	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	3	2	theme	lipid	542:546	arg1	profiles					550:557	subgingival lipid A profiles	530:557	subgingival lipid A profiles	530:557	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	3	3	dep	new	681:683	arg1	A-based					692:698	A-based	692:698	A-based	692:698	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	10	4	from	niche	1859:1863	arg1	present					1832:1838	present	1832:1838	present	1832:1838	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	3	5	theme	periodontal	742:752	arg1	therapy					754:760	personalized, point-of-care periodontal therapy	714:760	personalized, point-of-care periodontal therapy	714:760	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	3	6	theme	subgingival	530:540	arg1	profiles					550:557	subgingival lipid A profiles	530:557	subgingival lipid A profiles	530:557	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	1	7	theme	immune	264:269	arg1	system					271:276	bacteria-host immune system	250:276	bacteria-host immune system interactions	250:289	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	5	8	theme	moieties	969:976	arg1	composition					934:944	Chemical composition	925:944	Chemical composition of subgingival lipid A moieties	925:976	Chemical composition of subgingival lipid A moieties was determined by ESI-Mass Spectrometry.					
30543027	3	9	theme	endotoxin	563:571	arg1	activities					573:582	endotoxin activities	563:582	endotoxin activities	563:582	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	1	10	theme	lipid	175:179	arg1	domain					183:188	particularly its lipid A domain	158:188	particularly its lipid A domain	158:188	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	1	10	theme	lipid	175:179	arg1	Regulation					97:106	OBJECTIVES Regulation	86:106	OBJECTIVES Regulation of lipopolysaccharide (LPS)	86:134	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	4	11	theme	periodontal	904:914	arg1	therapy					916:922	periodontal therapy	904:922	periodontal therapy	904:922	MATERIALS AND METHODS Subgingival plaque samples were collected from healthy individuals and chronic periodontitis patients before and after periodontal therapy.					
30543027	9	12	theme	subgingival	1586:1596	arg1	extracts					1602:1609	subgingival LPS extracts	1586:1609	subgingival LPS extracts from periodontitis patients	1586:1637	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	6	13	theme	subgingival	1041:1051	arg1	extracts					1057:1064	subgingival LPS extracts	1041:1064	subgingival LPS extracts	1041:1064	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	10	14	theme	molecular	1888:1896	arg1	mechanisms					1909:1918	molecular pathogenic mechanisms	1888:1918	molecular pathogenic mechanisms of subgingival biofilm communities	1888:1953	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	9	15	theme	extracts	1602:1609	arg1	levels					1548:1553	Endotoxin activity levels	1529:1553	Endotoxin activity levels	1529:1553	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	9	15	theme	extracts	1602:1609	arg1	potentials					1572:1581	inflammatory potentials	1559:1581	inflammatory potentials	1559:1581	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	11	16	theme	derived	2084:2090	arg1	biomarker					2092:2100	bacterially derived biomarker	2072:2100	bacterially derived biomarker	2072:2100	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	10	17	theme	different	1805:1813	arg1	isoforms					1823:1830	different lipid A isoforms	1805:1830	different lipid A isoforms present in the subgingival niche	1805:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	11	18	theme	chemical	2029:2036	arg1	composition					2038:2048	lipid A chemical composition	2021:2048	lipid A chemical composition	2021:2048	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	9	19	theme	activity	1539:1546	arg1	levels					1548:1553	Endotoxin activity levels	1529:1553	Endotoxin activity levels	1529:1553	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	1	20	theme	important	197:205	arg1	mechanism					228:236	an important, naturally occurring mechanism	194:236	an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship	194:340	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	1	20	theme	important	197:205	arg1	Regulation					97:106	OBJECTIVES Regulation	86:106	OBJECTIVES Regulation of lipopolysaccharide (LPS)	86:134	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	11	21	theme	lipid	2021:2025	arg1	composition					2038:2048	lipid A chemical composition	2021:2048	lipid A chemical composition	2021:2048	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	3	22	theme	recombinant	649:659	arg1	assay					670:674	the recombinant factor C assay	645:674	the recombinant factor C assay	645:674	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	6	23	theme	factor	1101:1106	arg1	assay					1110:1114	the recombinant factor C assay	1085:1114	the recombinant factor C assay	1085:1114	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	1	24	dep	Regulation	97:106	arg1	composition					145:155	chemical composition	136:155	chemical composition	136:155	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	7	25	theme	A	1341:1341	arg1	isoforms					1343:1350	over-acylated, bi-phosphorylated lipid A isoforms	1302:1350	over-acylated, bi-phosphorylated lipid A isoforms	1302:1350	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	7	26	located	observed	1358:1365	arg2	signatures					1273:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	7	26	located	observed	1358:1365	arg1	samples					1379:1385	diseased samples	1370:1385	diseased samples	1370:1385	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	0	27	theme	A	18:18	arg1	profile					20:26	Subgingival lipid A profile	0:26	Subgingival lipid A profile	0:26	Subgingival lipid A profile and endotoxin activity in periodontal health and disease.					
30543027	5	28	theme	subgingival	949:959	arg1	moieties					969:976	subgingival lipid A moieties	949:976	subgingival lipid A moieties	949:976	Chemical composition of subgingival lipid A moieties was determined by ESI-Mass Spectrometry.					
30543027	8	29	theme	lower	1445:1449	arg1	peaks					1455:1459	lower m/z peaks	1445:1459	lower m/z peaks	1445:1459	Healthy and post-treatment samples were characterized by lower m/z peaks, related to under-acylated, hypo-phosphorylated lipid A structures.					
30543027	11	30	theme	Subgingival	1975:1985	arg1	activity					1997:2004	CLINICAL RELEVANCE Subgingival endotoxin activity	1956:2004	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition)	1956:2049	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	5	31	theme	A	967:967	arg1	moieties					969:976	subgingival lipid A moieties	949:976	subgingival lipid A moieties	949:976	Chemical composition of subgingival lipid A moieties was determined by ESI-Mass Spectrometry.					
30543027	4	32	theme	plaque	797:802	arg1	samples					804:810	Subgingival plaque samples	785:810	Subgingival plaque samples	785:810	MATERIALS AND METHODS Subgingival plaque samples were collected from healthy individuals and chronic periodontitis patients before and after periodontal therapy.					
30543027	11	33	theme	CLINICAL	1956:1963	arg1	RELEVANCE					1965:1973	CLINICAL RELEVANCE	1956:1973	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition)	1956:2049	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	7	34	theme	over-acylated	1302:1314	arg1	isoforms					1343:1350	over-acylated, bi-phosphorylated lipid A isoforms	1302:1350	over-acylated, bi-phosphorylated lipid A isoforms	1302:1350	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	10	35	theme	communities	1943:1953	arg1	mechanisms					1909:1918	molecular pathogenic mechanisms	1888:1918	molecular pathogenic mechanisms of subgingival biofilm communities	1888:1953	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	6	36	theme	extracts	1057:1064	arg1	activity					1029:1036	Endotoxin activity	1019:1036	Endotoxin activity of subgingival LPS extracts	1019:1064	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	0	37	from	activity	42:49	arg1	disease					77:83	disease	77:83	disease	77:83	Subgingival lipid A profile and endotoxin activity in periodontal health and disease.					
30543027	0	37	from	activity	42:49	arg1	health					66:71	periodontal health	54:71	periodontal health	54:71	Subgingival lipid A profile and endotoxin activity in periodontal health and disease.					
30543027	10	38	attach	present	1832:1838	arg1	niche					1859:1863	the subgingival niche	1843:1863	the subgingival niche	1843:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	10	38	attach	present	1832:1838	arg2	isoforms					1823:1830	different lipid A isoforms	1805:1830	different lipid A isoforms present in the subgingival niche	1805:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	11	39	theme	personalized	2133:2144	arg1	care					2158:2161	personalized periodontal care	2133:2161	personalized periodontal care	2133:2161	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	8	40	theme	related	1462:1468	arg1	peaks					1455:1459	lower m/z peaks	1445:1459	lower m/z peaks	1445:1459	Healthy and post-treatment samples were characterized by lower m/z peaks, related to under-acylated, hypo-phosphorylated lipid A structures.					
30543027	2	41	theme	host	410:413	arg1	responses					435:443	host immuno-inflammatory responses	410:443	host immuno-inflammatory responses	410:443	Members of the subgingival oral microbiota can critically modulate host immuno-inflammatory responses by synthesizing different LPS isoforms.					
30543027	0	42	theme	endotoxin	32:40	arg1	activity					42:49	endotoxin activity	32:49	endotoxin activity in periodontal health and disease	32:83	Subgingival lipid A profile and endotoxin activity in periodontal health and disease.					
30543027	8	43	theme	under-acylated	1473:1486	arg1	structures					1517:1526	under-acylated, hypo-phosphorylated lipid A structures	1473:1526	under-acylated, hypo-phosphorylated lipid A structures	1473:1526	Healthy and post-treatment samples were characterized by lower m/z peaks, related to under-acylated, hypo-phosphorylated lipid A structures.					
30543027	10	44	theme	isoforms	1823:1830	arg1	implications					1789:1800	structure-function-clinical implications	1761:1800	structure-function-clinical implications of different lipid A isoforms present in the subgingival niche	1761:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	0	45	theme	Subgingival	0:10	arg1	profile					20:26	Subgingival lipid A profile	0:26	Subgingival lipid A profile	0:26	Subgingival lipid A profile and endotoxin activity in periodontal health and disease.					
30543027	1	46	theme	system	271:276	arg1	interactions					278:289	bacteria-host immune system interactions	250:289	bacteria-host immune system interactions	250:289	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	1	47	theme	OBJECTIVES	86:95	arg1	mechanism					228:236	an important, naturally occurring mechanism	194:236	an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship	194:340	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	1	47	theme	OBJECTIVES	86:95	arg1	Regulation					97:106	OBJECTIVES Regulation	86:106	OBJECTIVES Regulation of lipopolysaccharide (LPS)	86:134	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	1	47	theme	OBJECTIVES	86:95	arg1	domain					183:188	particularly its lipid A domain	158:188	particularly its lipid A domain	158:188	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	6	48	theme	inflammatory	1127:1138	arg1	potential					1140:1148	their inflammatory potential	1121:1148	their inflammatory potential	1121:1148	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	2	49	theme	LPS	471:473	arg1	isoforms					475:482	different LPS isoforms	461:482	different LPS isoforms	461:482	Members of the subgingival oral microbiota can critically modulate host immuno-inflammatory responses by synthesizing different LPS isoforms.					
30543027	4	50	theme	chronic	856:862	arg1	patients					878:885	chronic periodontitis patients	856:885	chronic periodontitis patients	856:885	MATERIALS AND METHODS Subgingival plaque samples were collected from healthy individuals and chronic periodontitis patients before and after periodontal therapy.					
30543027	7	51	theme	A	1261:1261	arg1	signatures					1273:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	0	52	theme	periodontal	54:64	arg1	health					66:71	periodontal health	54:71	periodontal health	54:71	Subgingival lipid A profile and endotoxin activity in periodontal health and disease.					
30543027	11	53	theme	assessment	2113:2122	arg1	tool					2124:2127	a risk assessment tool	2106:2127	a risk assessment tool for personalized periodontal care	2106:2161	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	8	54	theme	A	1515:1515	arg1	structures					1517:1526	under-acylated, hypo-phosphorylated lipid A structures	1473:1526	under-acylated, hypo-phosphorylated lipid A structures	1473:1526	Healthy and post-treatment samples were characterized by lower m/z peaks, related to under-acylated, hypo-phosphorylated lipid A structures.					
30543027	10	55	theme	subgingival	1847:1857	arg1	niche					1859:1863	the subgingival niche	1843:1863	the subgingival niche	1843:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	7	56	theme	Characteristic	1240:1253	arg1	signatures					1273:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	3	57	theme	C	668:668	arg1	assay					670:674	the recombinant factor C assay	645:674	the recombinant factor C assay	645:674	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	11	58	theme	reliable	2062:2069	arg1	biomarker					2092:2100	bacterially derived biomarker	2072:2100	bacterially derived biomarker	2072:2100	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	3	59	theme	study	508:512	arg1	objectives					489:498	The objectives	485:498	The objectives of this study	485:512	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	10	60	theme	subgingival	1923:1933	arg1	communities					1943:1953	subgingival biofilm communities	1923:1953	subgingival biofilm communities	1923:1953	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	7	61	theme	diseased	1370:1377	arg1	samples					1379:1385	diseased samples	1370:1385	diseased samples	1370:1385	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	3	62	theme	new	681:683	arg1	biosensor					700:708	a new, lipid A-based biosensor	679:708	a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy	679:760	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	3	63	theme	A	548:548	arg1	profiles					550:557	subgingival lipid A profiles	530:557	subgingival lipid A profiles	530:557	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	3	64	theme	personalized	714:725	arg1	therapy					754:760	personalized, point-of-care periodontal therapy	714:760	personalized, point-of-care periodontal therapy	714:760	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	2	65	theme	subgingival	358:368	arg1	microbiota					375:384	the subgingival oral microbiota	354:384	the subgingival oral microbiota	354:384	Members of the subgingival oral microbiota can critically modulate host immuno-inflammatory responses by synthesizing different LPS isoforms.					
30543027	9	66	theme	Endotoxin	1529:1537	arg1	levels					1548:1553	Endotoxin activity levels	1529:1553	Endotoxin activity levels	1529:1553	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	10	67	theme	first	1737:1741	arg1	study					1743:1747	the first study	1733:1747	the first study	1733:1747	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	10	67	theme	first	1737:1741	arg1	This					1725:1728	This	1725:1728	This	1725:1728	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	1	68	theme	bacteria-host	250:262	arg1	system					271:276	bacteria-host immune system	250:276	bacteria-host immune system interactions	250:289	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	9	69	theme	healthy	1677:1683	arg1	samples					1704:1710	healthy and post-treatment samples	1677:1710	healthy and post-treatment samples	1677:1710	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	3	70	from	profiles	550:557	arg1	health					599:604	periodontal health	587:604	periodontal health	587:604	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	3	70	from	profiles	550:557	arg1	disease					610:616	disease	610:616	disease	610:616	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	10	71	theme	biofilm	1935:1941	arg1	communities					1943:1953	subgingival biofilm communities	1923:1953	subgingival biofilm communities	1923:1953	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	1	72	theme	A	181:181	arg1	domain					183:188	particularly its lipid A domain	158:188	particularly its lipid A domain	158:188	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	1	72	theme	A	181:181	arg1	Regulation					97:106	OBJECTIVES Regulation	86:106	OBJECTIVES Regulation of lipopolysaccharide (LPS)	86:134	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	9	73	theme	inflammatory	1559:1570	arg1	potentials					1572:1581	inflammatory potentials	1559:1581	inflammatory potentials	1559:1581	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	10	74	theme	structure-function-clinical	1761:1787	arg1	implications					1789:1800	structure-function-clinical implications	1761:1800	structure-function-clinical implications of different lipid A isoforms present in the subgingival niche	1761:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	5	75	theme	Chemical	925:932	arg1	composition					934:944	Chemical composition	925:944	Chemical composition of subgingival lipid A moieties	925:976	Chemical composition of subgingival lipid A moieties was determined by ESI-Mass Spectrometry.					
30543027	6	76	theme	LPS	1053:1055	arg1	extracts					1057:1064	subgingival LPS extracts	1041:1064	subgingival LPS extracts	1041:1064	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	9	77	from	patients	1630:1637	arg1	extracts					1602:1609	subgingival LPS extracts	1586:1609	subgingival LPS extracts from periodontitis patients	1586:1637	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	9	77	from	patients	1630:1637	arg1	levels					1548:1553	Endotoxin activity levels	1529:1553	Endotoxin activity levels	1529:1553	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	9	77	from	patients	1630:1637	arg1	potentials					1572:1581	inflammatory potentials	1559:1581	inflammatory potentials	1559:1581	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	3	78	theme	periodontal	587:597	arg1	health					599:604	periodontal health	587:604	periodontal health	587:604	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	9	79	theme	LPS	1598:1600	arg1	extracts					1602:1609	subgingival LPS extracts	1586:1609	subgingival LPS extracts from periodontitis patients	1586:1637	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	10	80	theme	lipid	1815:1819	arg1	isoforms					1823:1830	different lipid A isoforms	1805:1830	different lipid A isoforms present in the subgingival niche	1805:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	6	81	theme	Endotoxin	1019:1027	arg1	activity					1029:1036	Endotoxin activity	1019:1036	Endotoxin activity of subgingival LPS extracts	1019:1064	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	11	82	link	derived	2084:2090	arg1	biomarker					2092:2100	bacterially derived biomarker	2072:2100	bacterially derived biomarker	2072:2100	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	11	83	theme	periodontal	2146:2156	arg1	care					2158:2161	personalized periodontal care	2133:2161	personalized periodontal care	2133:2161	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	1	84	theme	lipopolysaccharide	111:128	arg1	mechanism					228:236	an important, naturally occurring mechanism	194:236	an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship	194:340	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	1	84	theme	lipopolysaccharide	111:128	arg1	Regulation					97:106	OBJECTIVES Regulation	86:106	OBJECTIVES Regulation of lipopolysaccharide (LPS)	86:134	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	1	84	theme	lipopolysaccharide	111:128	arg1	domain					183:188	particularly its lipid A domain	158:188	particularly its lipid A domain	158:188	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	8	85	theme	Healthy	1388:1394	arg1	samples					1415:1421	Healthy and post-treatment samples	1388:1421	Healthy and post-treatment samples	1388:1421	Healthy and post-treatment samples were characterized by lower m/z peaks, related to under-acylated, hypo-phosphorylated lipid A structures.					
30543027	11	86	theme	A	2027:2027	arg1	composition					2038:2048	lipid A chemical composition	2021:2048	lipid A chemical composition	2021:2048	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	10	87	dep	CONCLUSIONS	1713:1723	arg1	study					1743:1747	the first study	1733:1747	the first study	1733:1747	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	10	87	dep	CONCLUSIONS	1713:1723	arg1	This					1725:1728	This	1725:1728	This	1725:1728	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	4	88	dep	MATERIALS	763:771	arg1	samples					804:810	Subgingival plaque samples	785:810	Subgingival plaque samples	785:810	MATERIALS AND METHODS Subgingival plaque samples were collected from healthy individuals and chronic periodontitis patients before and after periodontal therapy.					
30543027	6	89	theme	C	1108:1108	arg1	assay					1110:1114	the recombinant factor C assay	1085:1114	the recombinant factor C assay	1085:1114	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	8	90	theme	post-treatment	1400:1413	arg1	samples					1415:1421	Healthy and post-treatment samples	1388:1421	Healthy and post-treatment samples	1388:1421	Healthy and post-treatment samples were characterized by lower m/z peaks, related to under-acylated, hypo-phosphorylated lipid A structures.					
30543027	10	91	theme	new	1875:1877	arg1	light					1879:1883	new light	1875:1883	new light	1875:1883	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	7	92	theme	lipid	1335:1339	arg1	A					1341:1341	lipid A	1335:1341	over-acylated, bi-phosphorylated lipid A isoforms	1302:1350	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	1	93	theme	chemical	136:143	arg1	composition					145:155	chemical composition	136:155	chemical composition	136:155	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	6	94	theme	recombinant	1089:1099	arg1	assay					1110:1114	the recombinant factor C assay	1085:1114	the recombinant factor C assay	1085:1114	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	0	95	from	profile	20:26	arg1	disease					77:83	disease	77:83	disease	77:83	Subgingival lipid A profile and endotoxin activity in periodontal health and disease.					
30543027	0	95	from	profile	20:26	arg1	health					66:71	periodontal health	54:71	periodontal health	54:71	Subgingival lipid A profile and endotoxin activity in periodontal health and disease.					
30543027	8	96	dep	under-acylated	1473:1486	arg1	hypo-phosphorylated					1489:1507	hypo-phosphorylated	1489:1507	hypo-phosphorylated	1489:1507	Healthy and post-treatment samples were characterized by lower m/z peaks, related to under-acylated, hypo-phosphorylated lipid A structures.					
30543027	5	97	theme	lipid	961:965	arg1	moieties					969:976	subgingival lipid A moieties	949:976	subgingival lipid A moieties	949:976	Chemical composition of subgingival lipid A moieties was determined by ESI-Mass Spectrometry.					
30543027	3	98	from	activities	573:582	arg1	health					599:604	periodontal health	587:604	periodontal health	587:604	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	3	98	from	activities	573:582	arg1	disease					610:616	disease	610:616	disease	610:616	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	11	99	theme	endotoxin	1987:1995	arg1	activity					1997:2004	CLINICAL RELEVANCE Subgingival endotoxin activity	1956:2004	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition)	1956:2049	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	9	100	theme	post-treatment	1689:1702	arg1	samples					1704:1710	healthy and post-treatment samples	1677:1710	healthy and post-treatment samples	1677:1710	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	8	101	theme	m/z	1451:1453	arg1	peaks					1455:1459	lower m/z peaks	1445:1459	lower m/z peaks	1445:1459	Healthy and post-treatment samples were characterized by lower m/z peaks, related to under-acylated, hypo-phosphorylated lipid A structures.					
30543027	4	102	theme	Subgingival	785:795	arg1	samples					804:810	Subgingival plaque samples	785:810	Subgingival plaque samples	785:810	MATERIALS AND METHODS Subgingival plaque samples were collected from healthy individuals and chronic periodontitis patients before and after periodontal therapy.					
30543027	11	103	theme	RELEVANCE	1965:1973	arg1	activity					1997:2004	CLINICAL RELEVANCE Subgingival endotoxin activity	1956:2004	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition)	1956:2049	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	10	104	theme	present	1832:1838	arg1	isoforms					1823:1830	different lipid A isoforms	1805:1830	different lipid A isoforms present in the subgingival niche	1805:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	0	105	theme	lipid	12:16	arg1	profile					20:26	Subgingival lipid A profile	0:26	Subgingival lipid A profile	0:26	Subgingival lipid A profile and endotoxin activity in periodontal health and disease.					
30543027	5	106	theme	ESI-Mass	996:1003	arg1	Spectrometry					1005:1016	ESI-Mass Spectrometry	996:1016	ESI-Mass Spectrometry	996:1016	Chemical composition of subgingival lipid A moieties was determined by ESI-Mass Spectrometry.					
30543027	10	107	theme	A	1821:1821	arg1	isoforms					1823:1830	different lipid A isoforms	1805:1830	different lipid A isoforms present in the subgingival niche	1805:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	7	108	theme	lipid	1255:1259	arg1	signatures					1273:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	9	109	from	levels	1548:1553	arg1	patients					1630:1637	periodontitis patients	1616:1637	periodontitis patients	1616:1637	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	2	110	theme	different	461:469	arg1	isoforms					475:482	different LPS isoforms	461:482	different LPS isoforms	461:482	Members of the subgingival oral microbiota can critically modulate host immuno-inflammatory responses by synthesizing different LPS isoforms.					
30543027	1	111	dep	relationship	329:340	arg1	either					296:301	either	296:301	either	296:301	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	3	112	theme	assay	670:674	arg1	use					638:640	the use	634:640	the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy	634:760	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	2	113	theme	microbiota	375:384	arg1	Members					343:349	Members	343:349	Members of the subgingival oral microbiota	343:384	Members of the subgingival oral microbiota can critically modulate host immuno-inflammatory responses by synthesizing different LPS isoforms.					
30543027	7	114	theme	RESULTS	1232:1238	arg1	signatures					1273:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	3	115	dep	personalized	714:725	arg1	point-of-care					728:740	point-of-care	728:740	point-of-care	728:740	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	11	116	dep	biomarker	2092:2100	arg1	a					2060:2060	a	2060:2060	a	2060:2060	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	3	117	theme	factor	661:666	arg1	assay					670:674	the recombinant factor C assay	645:674	the recombinant factor C assay	645:674	The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy.					
30543027	8	118	theme	lipid	1509:1513	arg1	structures					1517:1526	under-acylated, hypo-phosphorylated lipid A structures	1473:1526	under-acylated, hypo-phosphorylated lipid A structures	1473:1526	Healthy and post-treatment samples were characterized by lower m/z peaks, related to under-acylated, hypo-phosphorylated lipid A structures.					
30543027	2	119	theme	immuno-inflammatory	415:433	arg1	responses					435:443	host immuno-inflammatory responses	410:443	host immuno-inflammatory responses	410:443	Members of the subgingival oral microbiota can critically modulate host immuno-inflammatory responses by synthesizing different LPS isoforms.					
30543027	1	120	theme	symbiotic	305:313	arg1	relationship					329:340	either a symbiotic or pathogenic relationship	296:340	relationship	329:340	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	6	121	theme	IL-8	1215:1218	arg1	production					1220:1229	IL-8 production	1215:1229	IL-8 production	1215:1229	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	6	122	link	THP-1-derived	1166:1178	arg1	macrophages					1180:1190	THP-1-derived macrophages	1166:1190	THP-1-derived macrophages	1166:1190	Endotoxin activity of subgingival LPS extracts was assessed using the recombinant factor C assay, and their inflammatory potential was examined in THP-1-derived macrophages by measuring TNF-α and IL-8 production.					
30543027	4	123	theme	periodontitis	864:876	arg1	patients					878:885	chronic periodontitis patients	856:885	chronic periodontitis patients	856:885	MATERIALS AND METHODS Subgingival plaque samples were collected from healthy individuals and chronic periodontitis patients before and after periodontal therapy.					
30543027	1	124	theme	pathogenic	318:327	arg1	relationship					329:340	either a symbiotic or pathogenic relationship	296:340	relationship	329:340	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	10	125	from	present	1832:1838	arg1	niche					1859:1863	the subgingival niche	1843:1863	the subgingival niche	1843:1863	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	7	126	dep	over-acylated	1302:1314	arg1	bi-phosphorylated					1317:1333	bi-phosphorylated	1317:1333	bi-phosphorylated	1317:1333	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
30543027	1	127	dep	important	197:205	arg1	occurring					218:226	occurring	218:226	occurring	218:226	OBJECTIVES Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship.					
30543027	2	128	theme	oral	370:373	arg1	microbiota					375:384	the subgingival oral microbiota	354:384	the subgingival oral microbiota	354:384	Members of the subgingival oral microbiota can critically modulate host immuno-inflammatory responses by synthesizing different LPS isoforms.					
30543027	9	129	theme	periodontitis	1616:1628	arg1	patients					1630:1637	periodontitis patients	1616:1637	periodontitis patients	1616:1637	Endotoxin activity levels and inflammatory potentials of subgingival LPS extracts from periodontitis patients were significantly higher compared to healthy and post-treatment samples.					
30543027	10	130	theme	pathogenic	1898:1907	arg1	mechanisms					1909:1918	molecular pathogenic mechanisms	1888:1918	molecular pathogenic mechanisms of subgingival biofilm communities	1888:1953	CONCLUSIONS This is the first study to consider structure-function-clinical implications of different lipid A isoforms present in the subgingival niche and sheds new light on molecular pathogenic mechanisms of subgingival biofilm communities.					
30543027	11	131	theme	risk	2108:2111	arg1	tool					2124:2127	a risk assessment tool	2106:2127	a risk assessment tool for personalized periodontal care	2106:2161	CLINICAL RELEVANCE Subgingival endotoxin activity (determined by lipid A chemical composition) could be a reliable, bacterially derived biomarker and a risk assessment tool for personalized periodontal care.					
30543027	4	132	theme	healthy	832:838	arg1	individuals					840:850	healthy individuals	832:850	healthy individuals	832:850	MATERIALS AND METHODS Subgingival plaque samples were collected from healthy individuals and chronic periodontitis patients before and after periodontal therapy.					
30543027	7	133	theme	molecular	1263:1271	arg1	signatures					1273:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures	1232:1282	RESULTS Characteristic lipid A molecular signatures, corresponding to over-acylated, bi-phosphorylated lipid A isoforms, were observed in diseased samples.					
29719608	0	0	theme	CD147	101:105	arg1	glycosylation					107:119	CD147 glycosylation	101:119	CD147 glycosylation	101:119	c-Jun-dependent β3GnT8 promotes tumorigenesis and metastasis of hepatocellular carcinoma by inducing CD147 glycosylation and altering N-glycan patterns.					
29719608	1	1	theme	polylactosamine	167:181	arg1	β3GnT8					153:158	β3GnT8	153:158	β3GnT8	153:158	β3GnT8, a key polylactosamine synthase, plays a vital role in progression of various types of human cancer.					
29719608	1	1	theme	polylactosamine	167:181	arg1	synthase					183:190	a key polylactosamine synthase	161:190	a key polylactosamine synthase	161:190	β3GnT8, a key polylactosamine synthase, plays a vital role in progression of various types of human cancer.					
29719608	1	2	theme	cancer	253:258	arg1	types					238:242	various types	230:242	various types of human cancer	230:258	β3GnT8, a key polylactosamine synthase, plays a vital role in progression of various types of human cancer.					
29719608	6	3	from	structures	928:937	arg1	cells					946:950	HCC cells	942:950	HCC cells	942:950	Mechanistically, β3GnT8 modulated the N-glycosylation patterns of CD147 and altered the polylactosamine structures in HCC cells by physically interacting with CD147.					
29719608	6	4	theme	polylactosamine	912:926	arg1	structures					928:937	the polylactosamine structures	908:937	the polylactosamine structures in HCC cells	908:950	Mechanistically, β3GnT8 modulated the N-glycosylation patterns of CD147 and altered the polylactosamine structures in HCC cells by physically interacting with CD147.					
29719608	5	5	theme	HCC	735:737	arg1	cells					739:743	β3GnT8-overexpressing HCC cells	713:743	β3GnT8-overexpressing HCC cells	713:743	Importantly, enhanced tumorigenesis was observed in nude mice inoculated with β3GnT8-overexpressing HCC cells, suggesting that β3GnT8 is important for HCC development in vitro and in vivo.					
29719608	5	6	theme	enhanced	648:655	arg1	tumorigenesis					657:669	enhanced tumorigenesis	648:669	enhanced tumorigenesis	648:669	Importantly, enhanced tumorigenesis was observed in nude mice inoculated with β3GnT8-overexpressing HCC cells, suggesting that β3GnT8 is important for HCC development in vitro and in vivo.					
29719608	2	7	theme	hepatocellular	283:296	arg1	HCC					309:311	HCC	309:311	HCC	309:311	The role of β3GnT8 in hepatocellular carcinoma (HCC) and the underlying mechanisms, however, remain largely unknown.					
29719608	2	7	theme	hepatocellular	283:296	arg1	carcinoma					298:306	hepatocellular carcinoma	283:306	hepatocellular carcinoma (HCC)	283:312	The role of β3GnT8 in hepatocellular carcinoma (HCC) and the underlying mechanisms, however, remain largely unknown.					
29719608	2	8	from	mechanisms	333:342	arg1	HCC					309:311	HCC	309:311	HCC	309:311	The role of β3GnT8 in hepatocellular carcinoma (HCC) and the underlying mechanisms, however, remain largely unknown.					
29719608	2	8	from	mechanisms	333:342	arg1	carcinoma					298:306	hepatocellular carcinoma	283:306	hepatocellular carcinoma (HCC)	283:312	The role of β3GnT8 in hepatocellular carcinoma (HCC) and the underlying mechanisms, however, remain largely unknown.					
29719608	3	9	theme	HCC	459:461	arg1	tissues					463:469	HCC tissues	459:469	HCC tissues	459:469	In this study, we found that β3GnT8 and polylactosamine were highly expressed in HCC tissues compared with those in adjacent paracancer tissues.					
29719608	0	10	theme	N-glycan	134:141	arg1	patterns					143:150	N-glycan patterns	134:150	N-glycan patterns	134:150	c-Jun-dependent β3GnT8 promotes tumorigenesis and metastasis of hepatocellular carcinoma by inducing CD147 glycosylation and altering N-glycan patterns.					
29719608	2	11	theme	β3GnT8	273:278	arg1	mechanisms					333:342	the underlying mechanisms	318:342	the underlying mechanisms	318:342	The role of β3GnT8 in hepatocellular carcinoma (HCC) and the underlying mechanisms, however, remain largely unknown.					
29719608	2	11	theme	β3GnT8	273:278	arg1	role					265:268	The role	261:268	The role of β3GnT8 in hepatocellular carcinoma (HCC)	261:312	The role of β3GnT8 in hepatocellular carcinoma (HCC) and the underlying mechanisms, however, remain largely unknown.					
29719608	7	12	dep	showed	1012:1017	arg1	regulate					1084:1091	regulate	1084:1091	regulate β3GnT8 expression	1084:1109	In addition, our data showed the c-Jun could directly bind to the promoter of β3GnT8 gene and regulate β3GnT8 expression.					
29719608	7	12	dep	showed	1012:1017	arg1	bind					1044:1047	bind	1044:1047	bind to the promoter of β3GnT8 gene	1044:1078	In addition, our data showed the c-Jun could directly bind to the promoter of β3GnT8 gene and regulate β3GnT8 expression.					
29719608	4	13	theme	β3GnT8	576:581	arg1	knockdown					563:571	knockdown	563:571	knockdown of β3GnT8	563:581	Overexpression of β3GnT8 promoted while knockdown of β3GnT8 inhibited HCC cell invasion and migration in vitro.					
29719608	6	14	theme	CD147	890:894	arg1	patterns					878:885	the N-glycosylation patterns	858:885	the N-glycosylation patterns of CD147	858:894	Mechanistically, β3GnT8 modulated the N-glycosylation patterns of CD147 and altered the polylactosamine structures in HCC cells by physically interacting with CD147.					
29719608	7	15	theme	β3GnT8	1093:1098	arg1	expression					1100:1109	β3GnT8 expression	1093:1109	β3GnT8 expression	1093:1109	In addition, our data showed the c-Jun could directly bind to the promoter of β3GnT8 gene and regulate β3GnT8 expression.					
29719608	7	16	theme	β3GnT8	1068:1073	arg1	gene					1075:1078	β3GnT8 gene	1068:1078	β3GnT8 gene	1068:1078	In addition, our data showed the c-Jun could directly bind to the promoter of β3GnT8 gene and regulate β3GnT8 expression.					
29719608	1	17	theme	types	238:242	arg1	progression					215:225	progression	215:225	progression of various types of human cancer	215:258	β3GnT8, a key polylactosamine synthase, plays a vital role in progression of various types of human cancer.					
29719608	8	18	theme	C-Jun-dependent	1166:1180	arg1	manner					1182:1187	a C-Jun-dependent manner	1164:1187	a C-Jun-dependent manner	1164:1187	β3GnT8 regulated HCC cell invasion and migration in a C-Jun-dependent manner.					
29719608	7	19	theme	gene	1075:1078	arg1	promoter					1056:1063	the promoter	1052:1063	the promoter of β3GnT8 gene	1052:1078	In addition, our data showed the c-Jun could directly bind to the promoter of β3GnT8 gene and regulate β3GnT8 expression.					
29719608	0	20	theme	c-Jun-dependent	0:14	arg1	β3GnT8					16:21	c-Jun-dependent β3GnT8	0:21	c-Jun-dependent β3GnT8	0:21	c-Jun-dependent β3GnT8 promotes tumorigenesis and metastasis of hepatocellular carcinoma by inducing CD147 glycosylation and altering N-glycan patterns.					
29719608	4	21	theme	β3GnT8	541:546	arg1	Overexpression					523:536	Overexpression	523:536	Overexpression of β3GnT8	523:546	Overexpression of β3GnT8 promoted while knockdown of β3GnT8 inhibited HCC cell invasion and migration in vitro.					
29719608	5	22	located	observed	675:682	arg2	tumorigenesis					657:669	enhanced tumorigenesis	648:669	enhanced tumorigenesis	648:669	Importantly, enhanced tumorigenesis was observed in nude mice inoculated with β3GnT8-overexpressing HCC cells, suggesting that β3GnT8 is important for HCC development in vitro and in vivo.					
29719608	5	22	located	observed	675:682	arg1	mice					692:695	nude mice	687:695	nude mice inoculated with β3GnT8-overexpressing HCC cells	687:743	Importantly, enhanced tumorigenesis was observed in nude mice inoculated with β3GnT8-overexpressing HCC cells, suggesting that β3GnT8 is important for HCC development in vitro and in vivo.					
29719608	1	23	theme	vital	201:205	arg1	role					207:210	a vital role	199:210	a vital role	199:210	β3GnT8, a key polylactosamine synthase, plays a vital role in progression of various types of human cancer.					
29719608	3	24	theme	paracancer	503:512	arg1	tissues					514:520	adjacent paracancer tissues	494:520	adjacent paracancer tissues	494:520	In this study, we found that β3GnT8 and polylactosamine were highly expressed in HCC tissues compared with those in adjacent paracancer tissues.					
29719608	2	25	from	role	265:268	arg1	HCC					309:311	HCC	309:311	HCC	309:311	The role of β3GnT8 in hepatocellular carcinoma (HCC) and the underlying mechanisms, however, remain largely unknown.					
29719608	2	25	from	role	265:268	arg1	carcinoma					298:306	hepatocellular carcinoma	283:306	hepatocellular carcinoma (HCC)	283:312	The role of β3GnT8 in hepatocellular carcinoma (HCC) and the underlying mechanisms, however, remain largely unknown.					
29719608	6	26	gly	N-glycosylation	862:876	arg1	CD147					890:894	CD147	890:894	CD147	890:894	Mechanistically, β3GnT8 modulated the N-glycosylation patterns of CD147 and altered the polylactosamine structures in HCC cells by physically interacting with CD147.					
29719608	10	27	theme	Targeting	1289:1297	arg1	β3GnT8					1299:1304	Targeting β3GnT8	1289:1304	Targeting β3GnT8	1289:1304	Targeting β3GnT8 may be a potential therapeutic strategy against HCC.					
29719608	10	27	theme	Targeting	1289:1297	arg1	strategy					1337:1344	a potential therapeutic strategy	1313:1344	a potential therapeutic strategy against HCC	1313:1356	Targeting β3GnT8 may be a potential therapeutic strategy against HCC.					
29719608	6	28	theme	N-glycosylation	862:876	arg1	patterns					878:885	the N-glycosylation patterns	858:885	the N-glycosylation patterns of CD147	858:894	Mechanistically, β3GnT8 modulated the N-glycosylation patterns of CD147 and altered the polylactosamine structures in HCC cells by physically interacting with CD147.					
29719608	5	29	theme	HCC	786:788	arg1	development					790:800	HCC development	786:800	HCC development	786:800	Importantly, enhanced tumorigenesis was observed in nude mice inoculated with β3GnT8-overexpressing HCC cells, suggesting that β3GnT8 is important for HCC development in vitro and in vivo.					
29719608	5	30	theme	β3GnT8-overexpressing	713:733	arg1	cells					739:743	β3GnT8-overexpressing HCC cells	713:743	β3GnT8-overexpressing HCC cells	713:743	Importantly, enhanced tumorigenesis was observed in nude mice inoculated with β3GnT8-overexpressing HCC cells, suggesting that β3GnT8 is important for HCC development in vitro and in vivo.					
29719608	9	31	theme	novel	1237:1241	arg1	regulator					1243:1251	a novel regulator	1235:1251	a novel regulator for HCC invasion and tumorigenesis	1235:1286	Collectively, our study identified β3GnT8 as a novel regulator for HCC invasion and tumorigenesis.					
29719608	9	31	theme	novel	1237:1241	arg1	β3GnT8					1225:1230	β3GnT8	1225:1230	β3GnT8	1225:1230	Collectively, our study identified β3GnT8 as a novel regulator for HCC invasion and tumorigenesis.					
29719608	0	32	theme	hepatocellular	64:77	arg1	carcinoma					79:87	hepatocellular carcinoma	64:87	hepatocellular carcinoma	64:87	c-Jun-dependent β3GnT8 promotes tumorigenesis and metastasis of hepatocellular carcinoma by inducing CD147 glycosylation and altering N-glycan patterns.					
29719608	8	33	theme	HCC	1129:1131	arg1	invasion					1138:1145	HCC cell invasion	1129:1145	HCC cell invasion	1129:1145	β3GnT8 regulated HCC cell invasion and migration in a C-Jun-dependent manner.					
29719608	1	34	theme	key	163:165	arg1	β3GnT8					153:158	β3GnT8	153:158	β3GnT8	153:158	β3GnT8, a key polylactosamine synthase, plays a vital role in progression of various types of human cancer.					
29719608	1	34	theme	key	163:165	arg1	synthase					183:190	a key polylactosamine synthase	161:190	a key polylactosamine synthase	161:190	β3GnT8, a key polylactosamine synthase, plays a vital role in progression of various types of human cancer.					
29719608	10	35	dep	potential	1315:1323	arg1	therapeutic					1325:1335	therapeutic	1325:1335	therapeutic	1325:1335	Targeting β3GnT8 may be a potential therapeutic strategy against HCC.					
29719608	2	36	theme	underlying	322:331	arg1	mechanisms					333:342	the underlying mechanisms	318:342	the underlying mechanisms	318:342	The role of β3GnT8 in hepatocellular carcinoma (HCC) and the underlying mechanisms, however, remain largely unknown.					
29719608	6	37	theme	HCC	942:944	arg1	cells					946:950	HCC cells	942:950	HCC cells	942:950	Mechanistically, β3GnT8 modulated the N-glycosylation patterns of CD147 and altered the polylactosamine structures in HCC cells by physically interacting with CD147.					
29719608	9	38	theme	HCC	1257:1259	arg1	invasion					1261:1268	HCC invasion	1257:1268	HCC invasion	1257:1268	Collectively, our study identified β3GnT8 as a novel regulator for HCC invasion and tumorigenesis.					
29719608	4	39	theme	cell	597:600	arg1	invasion					602:609	HCC cell invasion	593:609	HCC cell invasion	593:609	Overexpression of β3GnT8 promoted while knockdown of β3GnT8 inhibited HCC cell invasion and migration in vitro.					
29719608	5	40	theme	nude	687:690	arg1	mice					692:695	nude mice	687:695	nude mice inoculated with β3GnT8-overexpressing HCC cells	687:743	Importantly, enhanced tumorigenesis was observed in nude mice inoculated with β3GnT8-overexpressing HCC cells, suggesting that β3GnT8 is important for HCC development in vitro and in vivo.					
29719608	3	41	theme	adjacent	494:501	arg1	tissues					514:520	adjacent paracancer tissues	494:520	adjacent paracancer tissues	494:520	In this study, we found that β3GnT8 and polylactosamine were highly expressed in HCC tissues compared with those in adjacent paracancer tissues.					
29719608	4	42	theme	HCC	593:595	arg1	invasion					602:609	HCC cell invasion	593:609	HCC cell invasion	593:609	Overexpression of β3GnT8 promoted while knockdown of β3GnT8 inhibited HCC cell invasion and migration in vitro.					
29719608	8	43	theme	cell	1133:1136	arg1	invasion					1138:1145	HCC cell invasion	1129:1145	HCC cell invasion	1129:1145	β3GnT8 regulated HCC cell invasion and migration in a C-Jun-dependent manner.					
29719608	10	44	theme	potential	1315:1323	arg1	β3GnT8					1299:1304	Targeting β3GnT8	1289:1304	Targeting β3GnT8	1289:1304	Targeting β3GnT8 may be a potential therapeutic strategy against HCC.					
29719608	10	44	theme	potential	1315:1323	arg1	strategy					1337:1344	a potential therapeutic strategy	1313:1344	a potential therapeutic strategy against HCC	1313:1356	Targeting β3GnT8 may be a potential therapeutic strategy against HCC.					
29719608	0	45	theme	carcinoma	79:87	arg1	metastasis					50:59	metastasis	50:59	metastasis	50:59	c-Jun-dependent β3GnT8 promotes tumorigenesis and metastasis of hepatocellular carcinoma by inducing CD147 glycosylation and altering N-glycan patterns.					
29719608	0	45	theme	carcinoma	79:87	arg1	tumorigenesis					32:44	tumorigenesis	32:44	tumorigenesis	32:44	c-Jun-dependent β3GnT8 promotes tumorigenesis and metastasis of hepatocellular carcinoma by inducing CD147 glycosylation and altering N-glycan patterns.					
29719608	1	46	theme	various	230:236	arg1	types					238:242	various types	230:242	various types of human cancer	230:258	β3GnT8, a key polylactosamine synthase, plays a vital role in progression of various types of human cancer.					
29719608	1	47	theme	human	247:251	arg1	cancer					253:258	human cancer	247:258	human cancer	247:258	β3GnT8, a key polylactosamine synthase, plays a vital role in progression of various types of human cancer.					
30444002	3	0	theme	healthy	669:675	arg1	fascia					677:682	healthy fascia	669:682	healthy fascia	669:682	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	10	1	from	variation	1626:1634	arg1	contents					1650:1657	hyaluronan contents	1639:1657	hyaluronan contents	1639:1657	This work demonstrates a variation in hyaluronan contents around the chondrosarcoma, likely correlated with the aggressiveness and resistance to chemotherapy of this tumors.					
30444002	6	2	theme	statistical	1141:1151	arg1	difference					1153:1162	a highly significant statistical difference	1120:1162	a highly significant statistical difference	1120:1162	The p-value was less than 0.01, showing a highly significant statistical difference.					
30444002	3	3	theme	district	705:712	arg1	tumor					633:637	the tumor	629:637	the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district	629:712	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	3	4	theme	extracellular	533:545	arg1	matrix					547:552	the extracellular matrix	529:552	the extracellular matrix	529:552	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	3	5	theme	same	691:694	arg1	district					705:712	the same anatomic district	687:712	the same anatomic district	687:712	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	11	6	theme	metastatic	1958:1967	arg1	potential					1969:1977	the metastatic potential	1954:1977	the metastatic potential of tumors and their prognostic indices	1954:2016	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	4	7	theme	healthy	842:848	arg1	fascia					850:855	the healthy fascia	838:855	the healthy fascia	838:855	We demonstrate that hyaluronan increased significantly in the peritumoral stroma compared with the healthy fascia, which showed an average amount according to the physical characteristics of body districts by a mean value of 26.9 μg/g.					
30444002	11	8	theme	prognostic	1999:2008	arg1	indices					2010:2016	their prognostic indices	1993:2016	their prognostic indices	1993:2016	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	12	9	theme	Orthopaedic	2026:2036	arg1	Research					2038:2045	© 2018 Orthopaedic Research	2019:2045	© 2018 Orthopaedic Research Society.	2019:2054	© 2018 Orthopaedic Research Society.					
30444002	11	10	theme	indices	2010:2016	arg1	potential					1969:1977	the metastatic potential	1954:1977	the metastatic potential of tumors and their prognostic indices	1954:2016	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	5	11	theme	mean	1054:1057	arg1	value					1059:1063	mean value	1054:1063	mean value of 63.2 μg/g	1054:1076	In the peritumoral stroma, the mean hyaluronan content reached 132.6 μg/g (mean value of 63.2 μg/g).					
30444002	4	12	theme	average	874:880	arg1	amount					882:887	an average amount	871:887	an average amount according to the physical characteristics of body districts	871:947	We demonstrate that hyaluronan increased significantly in the peritumoral stroma compared with the healthy fascia, which showed an average amount according to the physical characteristics of body districts by a mean value of 26.9 μg/g.					
30444002	8	13	theme	necrosis	1399:1406	arg1	state					1390:1394	the state	1386:1394	the state of necrosis	1386:1406	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
30444002	5	14	theme	mean	1010:1013	arg1	content					1026:1032	the mean hyaluronan content	1006:1032	the mean hyaluronan content	1006:1032	In the peritumoral stroma, the mean hyaluronan content reached 132.6 μg/g (mean value of 63.2 μg/g).					
30444002	1	15	theme	recent	125:130	arg1	years					132:136	recent years	125:136	recent years	125:136	A concept consolidated in recent years is that tumor growth depends to a great extent on the micro-environment surrounding the tumor, which has a fundamental role in tumor progression and in determining the effectiveness of therapies.					
30444002	11	16	from	matrix	1899:1904	arg1	rich					1877:1880	rich	1877:1880	rich	1877:1880	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	3	17	theme	connective	599:608	arg1	tissue					610:615	the connective tissue	595:615	the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district	595:712	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	4	18	theme	mean	954:957	arg1	value					959:963	a mean value	952:963	a mean value of 26.9 μg/g	952:976	We demonstrate that hyaluronan increased significantly in the peritumoral stroma compared with the healthy fascia, which showed an average amount according to the physical characteristics of body districts by a mean value of 26.9 μg/g.					
30444002	1	19	theme	tumor	146:150	arg1	growth					152:157	tumor growth	146:157	tumor growth	146:157	A concept consolidated in recent years is that tumor growth depends to a great extent on the micro-environment surrounding the tumor, which has a fundamental role in tumor progression and in determining the effectiveness of therapies.					
30444002	11	20	theme	stroma	1869:1874	arg1	composition					1838:1848	the composition	1834:1848	the composition of the peritumoral stroma	1834:1874	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	1	21	contain	has	239:241	arg2	role					257:260	a fundamental role	243:260	a fundamental role	243:260	A concept consolidated in recent years is that tumor growth depends to a great extent on the micro-environment surrounding the tumor, which has a fundamental role in tumor progression and in determining the effectiveness of therapies.					
30444002	1	21	contain	has	239:241	arg1	tumor					226:230	the tumor	222:230	the tumor	222:230	A concept consolidated in recent years is that tumor growth depends to a great extent on the micro-environment surrounding the tumor, which has a fundamental role in tumor progression and in determining the effectiveness of therapies.					
30444002	9	22	theme	acid	1459:1462	arg1	synthetase					1464:1473	hyaluronic acid synthetase	1448:1473	hyaluronic acid synthetase	1448:1473	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	12	23	theme	2018	2021:2024	arg1	Research					2038:2045	© 2018 Orthopaedic Research	2019:2045	© 2018 Orthopaedic Research Society.	2019:2054	© 2018 Orthopaedic Research Society.					
30444002	0	24	theme	human	77:81	arg1	chondrosarcoma					83:96	human chondrosarcoma	77:96	human chondrosarcoma	77:96	Variations in contents of hyaluronan in the peritumoral micro-environment of human chondrosarcoma.					
30444002	4	25	theme	body	934:937	arg1	districts					939:947	body districts	934:947	body districts	934:947	We demonstrate that hyaluronan increased significantly in the peritumoral stroma compared with the healthy fascia, which showed an average amount according to the physical characteristics of body districts by a mean value of 26.9 μg/g.					
30444002	0	26	from	Variations	0:9	arg1	contents					14:21	contents	14:21	contents of hyaluronan	14:35	Variations in contents of hyaluronan in the peritumoral micro-environment of human chondrosarcoma.					
30444002	0	26	from	Variations	0:9	arg1	micro-environment					56:72	the peritumoral micro-environment	40:72	the peritumoral micro-environment of human chondrosarcoma	40:96	Variations in contents of hyaluronan in the peritumoral micro-environment of human chondrosarcoma.					
30444002	3	27	dep	quantified	476:485	arg1	viewed					715:720	viewed	715:720	viewed as a health control	715:740	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	2	28	theme	primary	385:391	arg1	tumor					408:412	the second primary malignant bone tumor	374:412	the second primary malignant bone tumor	374:412	Our analysis focuses on chondrosarcoma, the second primary malignant bone tumor, resistant to both chemotherapeutic and radiation therapy.					
30444002	2	28	theme	primary	385:391	arg1	chondrosarcoma					358:371	chondrosarcoma	358:371	chondrosarcoma	358:371	Our analysis focuses on chondrosarcoma, the second primary malignant bone tumor, resistant to both chemotherapeutic and radiation therapy.					
30444002	9	29	theme	%	1504:1504	arg1	decrease					1484:1491	a decrease	1482:1491	a decrease of about 50% in peritumoral and tumor tissues	1482:1537	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	11	30	theme	extracellular	1885:1897	arg1	matrix					1899:1904	extracellular matrix	1885:1904	extracellular matrix	1885:1904	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	7	31	theme	grade	1259:1263	arg1	function					1225:1232	a function	1223:1232	a function of age, gender, or tumor grade	1223:1263	Surprisingly, no significant differences were detected as a function of age, gender, or tumor grade.					
30444002	7	31	theme	grade	1259:1263	arg1	differences					1194:1204	no significant differences	1179:1204	no significant differences	1179:1204	Surprisingly, no significant differences were detected as a function of age, gender, or tumor grade.					
30444002	2	32	theme	bone	403:406	arg1	tumor					408:412	the second primary malignant bone tumor	374:412	the second primary malignant bone tumor	374:412	Our analysis focuses on chondrosarcoma, the second primary malignant bone tumor, resistant to both chemotherapeutic and radiation therapy.					
30444002	2	32	theme	bone	403:406	arg1	chondrosarcoma					358:371	chondrosarcoma	358:371	chondrosarcoma	358:371	Our analysis focuses on chondrosarcoma, the second primary malignant bone tumor, resistant to both chemotherapeutic and radiation therapy.					
30444002	11	33	theme	rich	1877:1880	arg1	knowledge					1818:1826	Deeper knowledge	1811:1826	Deeper knowledge	1811:1826	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	0	34	theme	hyaluronan	26:35	arg1	contents					14:21	contents	14:21	contents of hyaluronan	14:35	Variations in contents of hyaluronan in the peritumoral micro-environment of human chondrosarcoma.					
30444002	9	35	theme	tumor	1525:1529	arg1	tissues					1531:1537	tumor tissues	1525:1537	tumor tissues	1525:1537	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	2	36	theme	radiation	454:462	arg1	therapy					464:470	radiation therapy	454:470	radiation therapy	454:470	Our analysis focuses on chondrosarcoma, the second primary malignant bone tumor, resistant to both chemotherapeutic and radiation therapy.					
30444002	11	37	theme	Deeper	1811:1816	arg1	knowledge					1818:1826	Deeper knowledge	1811:1826	Deeper knowledge	1811:1826	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	7	38	theme	gender	1242:1247	arg1	function					1225:1232	a function	1223:1232	a function of age, gender, or tumor grade	1223:1263	Surprisingly, no significant differences were detected as a function of age, gender, or tumor grade.					
30444002	7	38	theme	gender	1242:1247	arg1	differences					1194:1204	no significant differences	1179:1204	no significant differences	1179:1204	Surprisingly, no significant differences were detected as a function of age, gender, or tumor grade.					
30444002	11	39	theme	Significance	1797:1808	arg1	Statement					1775:1783	Statement	1775:1783	Statement of Clinical Significance	1775:1808	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	8	40	from	comparable	1296:1305	arg1	peritumoral					1310:1320	peritumoral	1310:1320	peritumoral	1310:1320	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
30444002	8	40	from	comparable	1296:1305	arg1	tissues					1332:1338	tumor tissues	1326:1338	tumor tissues	1326:1338	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
30444002	7	41	theme	age	1237:1239	arg1	function					1225:1232	a function	1223:1232	a function of age, gender, or tumor grade	1223:1263	Surprisingly, no significant differences were detected as a function of age, gender, or tumor grade.					
30444002	7	41	theme	age	1237:1239	arg1	differences					1194:1204	no significant differences	1179:1204	no significant differences	1179:1204	Surprisingly, no significant differences were detected as a function of age, gender, or tumor grade.					
30444002	3	42	theme	intra-tumoral	644:656	arg1	tissue					658:663	intra-tumoral tissue	644:663	intra-tumoral tissue	644:663	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	9	43	theme	hyaluronan	1566:1575	arg1	turnover					1577:1584	hyaluronan turnover	1566:1584	hyaluronan turnover	1566:1584	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	11	44	theme	potential	1969:1977	arg1	understanding					1937:1949	study and understanding	1927:1949	understanding	1937:1949	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	11	44	theme	potential	1969:1977	arg1	study					1927:1931	study and understanding	1927:1949	study	1927:1931	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	8	45	from	peritumoral	1310:1320	arg1	comparable					1296:1305	comparable	1296:1305	comparable	1296:1305	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
30444002	8	45	from	peritumoral	1310:1320	arg1	levels					1270:1275	The levels	1266:1275	The levels of hyaluronan	1266:1289	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
30444002	1	46	theme	fundamental	245:255	arg1	role					257:260	a fundamental role	243:260	a fundamental role	243:260	A concept consolidated in recent years is that tumor growth depends to a great extent on the micro-environment surrounding the tumor, which has a fundamental role in tumor progression and in determining the effectiveness of therapies.					
30444002	6	47	theme	significant	1129:1139	arg1	difference					1153:1162	a highly significant statistical difference	1120:1162	a highly significant statistical difference	1120:1162	The p-value was less than 0.01, showing a highly significant statistical difference.					
30444002	3	48	theme	components	515:524	arg1	hyaluronan					487:496	hyaluronan	487:496	hyaluronan	487:496	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	3	48	theme	components	515:524	arg1	components					515:524	the main components	506:524	the main components of the extracellular matrix	506:552	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	3	48	theme	components	515:524	arg1	one					499:501	one	499:501	one	499:501	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	3	49	theme	anatomic	696:703	arg1	district					705:712	the same anatomic district	687:712	the same anatomic district	687:712	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	7	50	theme	significant	1182:1192	arg1	function					1225:1232	a function	1223:1232	a function of age, gender, or tumor grade	1223:1263	Surprisingly, no significant differences were detected as a function of age, gender, or tumor grade.					
30444002	7	50	theme	significant	1182:1192	arg1	differences					1194:1204	no significant differences	1179:1204	no significant differences	1179:1204	Surprisingly, no significant differences were detected as a function of age, gender, or tumor grade.					
30444002	1	51	theme	great	172:176	arg1	extent					178:183	a great extent	170:183	a great extent	170:183	A concept consolidated in recent years is that tumor growth depends to a great extent on the micro-environment surrounding the tumor, which has a fundamental role in tumor progression and in determining the effectiveness of therapies.					
30444002	3	52	theme	health	727:732	arg1	control					734:740	a health control	725:740	a health control	725:740	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	3	53	theme	matrix	547:552	arg1	components					515:524	the main components	506:524	the main components of the extracellular matrix	506:552	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	10	54	theme	tumors	1767:1772	arg1	resistance					1732:1741	resistance	1732:1741	resistance to chemotherapy	1732:1757	This work demonstrates a variation in hyaluronan contents around the chondrosarcoma, likely correlated with the aggressiveness and resistance to chemotherapy of this tumors.					
30444002	10	54	theme	tumors	1767:1772	arg1	aggressiveness					1713:1726	aggressiveness	1713:1726	aggressiveness	1713:1726	This work demonstrates a variation in hyaluronan contents around the chondrosarcoma, likely correlated with the aggressiveness and resistance to chemotherapy of this tumors.					
30444002	3	55	with	tumor	633:637	arg1	tissue					658:663	intra-tumoral tissue	644:663	intra-tumoral tissue	644:663	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	3	55	with	tumor	633:637	arg1	fascia					677:682	healthy fascia	669:682	healthy fascia	669:682	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	4	56	theme	physical	906:913	arg1	characteristics					915:929	the physical characteristics	902:929	the physical characteristics of body districts	902:947	We demonstrate that hyaluronan increased significantly in the peritumoral stroma compared with the healthy fascia, which showed an average amount according to the physical characteristics of body districts by a mean value of 26.9 μg/g.					
30444002	11	57	from	rich	1877:1880	arg1	matrix					1899:1904	extracellular matrix	1885:1904	extracellular matrix	1885:1904	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	11	58	theme	Clinical	1788:1795	arg1	Significance					1797:1808	Clinical Significance	1788:1808	Clinical Significance	1788:1808	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	10	59	dep	aggressiveness	1713:1726	arg1	the					1709:1711	the	1709:1711	the	1709:1711	This work demonstrates a variation in hyaluronan contents around the chondrosarcoma, likely correlated with the aggressiveness and resistance to chemotherapy of this tumors.					
30444002	5	60	theme	hyaluronan	1015:1024	arg1	content					1026:1032	the mean hyaluronan content	1006:1032	the mean hyaluronan content	1006:1032	In the peritumoral stroma, the mean hyaluronan content reached 132.6 μg/g (mean value of 63.2 μg/g).					
30444002	11	61	theme	tumors	1982:1987	arg1	potential					1969:1977	the metastatic potential	1954:1977	the metastatic potential of tumors and their prognostic indices	1954:2016	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	9	62	theme	hyaluronic	1448:1457	arg1	synthetase					1464:1473	hyaluronic acid synthetase	1448:1473	hyaluronic acid synthetase	1448:1473	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	0	63	theme	chondrosarcoma	83:96	arg1	micro-environment					56:72	the peritumoral micro-environment	40:72	the peritumoral micro-environment of human chondrosarcoma	40:96	Variations in contents of hyaluronan in the peritumoral micro-environment of human chondrosarcoma.					
30444002	1	64	theme	therapies	323:331	arg1	effectiveness					306:318	the effectiveness	302:318	the effectiveness of therapies	302:331	A concept consolidated in recent years is that tumor growth depends to a great extent on the micro-environment surrounding the tumor, which has a fundamental role in tumor progression and in determining the effectiveness of therapies.					
30444002	9	65	theme	synthetase	1464:1473	arg1	expression					1434:1443	the expression	1430:1443	the expression of hyaluronic acid synthetase	1430:1473	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	12	66	theme	©	2019:2019	arg1	Research					2038:2045	© 2018 Orthopaedic Research	2019:2045	© 2018 Orthopaedic Research Society.	2019:2054	© 2018 Orthopaedic Research Society.					
30444002	9	67	from	decrease	1484:1491	arg1	tissues					1531:1537	tumor tissues	1525:1537	tumor tissues	1525:1537	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	9	67	from	decrease	1484:1491	arg1	peritumoral					1509:1519	peritumoral	1509:1519	peritumoral	1509:1519	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	8	68	theme	hyaluronan	1280:1289	arg1	comparable					1296:1305	comparable	1296:1305	comparable	1296:1305	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
30444002	8	68	theme	hyaluronan	1280:1289	arg1	levels					1270:1275	The levels	1266:1275	The levels of hyaluronan	1266:1289	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
30444002	3	69	from	amount	585:590	arg1	tissue					610:615	the connective tissue	595:615	the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district	595:712	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	5	70	theme	63.2 μg/g	1068:1076	arg1	value					1059:1063	mean value	1054:1063	mean value of 63.2 μg/g	1054:1076	In the peritumoral stroma, the mean hyaluronan content reached 132.6 μg/g (mean value of 63.2 μg/g).					
30444002	4	71	theme	districts	939:947	arg1	characteristics					915:929	the physical characteristics	902:929	the physical characteristics of body districts	902:947	We demonstrate that hyaluronan increased significantly in the peritumoral stroma compared with the healthy fascia, which showed an average amount according to the physical characteristics of body districts by a mean value of 26.9 μg/g.					
30444002	2	72	theme	second	378:383	arg1	tumor					408:412	the second primary malignant bone tumor	374:412	the second primary malignant bone tumor	374:412	Our analysis focuses on chondrosarcoma, the second primary malignant bone tumor, resistant to both chemotherapeutic and radiation therapy.					
30444002	2	72	theme	second	378:383	arg1	chondrosarcoma					358:371	chondrosarcoma	358:371	chondrosarcoma	358:371	Our analysis focuses on chondrosarcoma, the second primary malignant bone tumor, resistant to both chemotherapeutic and radiation therapy.					
30444002	9	73	from	alterations	1551:1561	arg1	synthesis					1590:1598	synthesis	1590:1598	synthesis	1590:1598	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	9	73	from	alterations	1551:1561	arg1	turnover					1577:1584	hyaluronan turnover	1566:1584	hyaluronan turnover	1566:1584	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	11	74	theme	peritumoral	1857:1867	arg1	stroma					1869:1874	the peritumoral stroma	1853:1874	the peritumoral stroma	1853:1874	Statement of Clinical Significance: Deeper knowledge about the composition of the peritumoral stroma, rich in extracellular matrix, will enhance better study and understanding of the metastatic potential of tumors and their prognostic indices.					
30444002	1	75	theme	tumor	265:269	arg1	progression					271:281	tumor progression	265:281	tumor progression	265:281	A concept consolidated in recent years is that tumor growth depends to a great extent on the micro-environment surrounding the tumor, which has a fundamental role in tumor progression and in determining the effectiveness of therapies.					
30444002	7	76	theme	tumor	1253:1257	arg1	grade					1259:1263	tumor grade	1253:1263	tumor grade	1253:1263	Surprisingly, no significant differences were detected as a function of age, gender, or tumor grade.					
30444002	8	77	theme	tumor	1326:1330	arg1	tissues					1332:1338	tumor tissues	1326:1338	tumor tissues	1326:1338	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
30444002	5	78	theme	peritumoral	986:996	arg1	stroma					998:1003	the peritumoral stroma	982:1003	the peritumoral stroma	982:1003	In the peritumoral stroma, the mean hyaluronan content reached 132.6 μg/g (mean value of 63.2 μg/g).					
30444002	4	79	theme	26.9 μg/g	968:976	arg1	value					959:963	a mean value	952:963	a mean value of 26.9 μg/g	952:976	We demonstrate that hyaluronan increased significantly in the peritumoral stroma compared with the healthy fascia, which showed an average amount according to the physical characteristics of body districts by a mean value of 26.9 μg/g.					
30444002	2	80	theme	malignant	393:401	arg1	tumor					408:412	the second primary malignant bone tumor	374:412	the second primary malignant bone tumor	374:412	Our analysis focuses on chondrosarcoma, the second primary malignant bone tumor, resistant to both chemotherapeutic and radiation therapy.					
30444002	2	80	theme	malignant	393:401	arg1	chondrosarcoma					358:371	chondrosarcoma	358:371	chondrosarcoma	358:371	Our analysis focuses on chondrosarcoma, the second primary malignant bone tumor, resistant to both chemotherapeutic and radiation therapy.					
30444002	0	81	theme	peritumoral	44:54	arg1	micro-environment					56:72	the peritumoral micro-environment	40:72	the peritumoral micro-environment of human chondrosarcoma	40:96	Variations in contents of hyaluronan in the peritumoral micro-environment of human chondrosarcoma.					
30444002	9	82	from	data	1422:1425	arg1	expression					1434:1443	the expression	1430:1443	the expression of hyaluronic acid synthetase	1430:1473	In addition, data on the expression of hyaluronic acid synthetase showed a decrease of about 50% in peritumoral and tumor tissues, indicating alterations in hyaluronan turnover and synthesis.					
30444002	4	83	theme	peritumoral	805:815	arg1	stroma					817:822	the peritumoral stroma	801:822	the peritumoral stroma	801:822	We demonstrate that hyaluronan increased significantly in the peritumoral stroma compared with the healthy fascia, which showed an average amount according to the physical characteristics of body districts by a mean value of 26.9 μg/g.					
30444002	10	84	theme	hyaluronan	1639:1648	arg1	contents					1650:1657	hyaluronan contents	1639:1657	hyaluronan contents	1639:1657	This work demonstrates a variation in hyaluronan contents around the chondrosarcoma, likely correlated with the aggressiveness and resistance to chemotherapy of this tumors.					
30444002	5	85	dep	132.6 μg/g	1042:1051	arg1	value					1059:1063	mean value	1054:1063	mean value of 63.2 μg/g	1054:1076	In the peritumoral stroma, the mean hyaluronan content reached 132.6 μg/g (mean value of 63.2 μg/g).					
30444002	3	86	theme	main	510:513	arg1	components					515:524	the main components	506:524	the main components of the extracellular matrix	506:552	We quantified hyaluronan, one of the main components of the extracellular matrix, with the aim of comparing its amount in the connective tissue surrounding the tumor with intra-tumoral tissue and healthy fascia of the same anatomic district, viewed as a health control.					
30444002	8	87	from	tissues	1332:1338	arg1	comparable					1296:1305	comparable	1296:1305	comparable	1296:1305	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
30444002	8	87	from	tissues	1332:1338	arg1	levels					1270:1275	The levels	1266:1275	The levels of hyaluronan	1266:1289	The levels of hyaluronan were comparable in peritumoral and tumor tissues, although there were differences depending on the state of necrosis.					
29935832	3	0	from	composition	485:495	arg1	effects					398:404	the effects	394:404	the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage	394:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	10	1	theme	lowest	1550:1555	arg1	value					1557:1561	the lowest value	1546:1561	the lowest value of gas losses	1546:1575	The combination of CHI+INO showed the lowest value of gas losses.					
29935832	8	2	from	concentration	1404:1416	arg1	SS					1421:1422	SS	1421:1422	SS	1421:1422	Chitosan increased NH3-N and lactate concentrations and decreased ethanol concentration in SS.					
29935832	7	3	theme	carbohydrate	1302:1313	arg1	content					1315:1321	nonfiber carbohydrate content	1293:1321	nonfiber carbohydrate content of SS	1293:1327	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	9	4	theme	silage	1486:1491	arg1	stability					1501:1509	silage aerobic stability	1486:1509	silage aerobic stability	1486:1509	The CHI increased dry matter recovery from SS; INO increased silage aerobic stability.					
29935832	7	5	theme	neutral	1254:1260	arg1	fiber					1272:1276	neutral detergent fiber	1254:1276	neutral detergent fiber	1254:1276	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	3	6	theme	fermentative	529:540	arg1	profile					542:548	fermentative profile	529:548	fermentative profile	529:548	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	11	7	from	yeast	1762:1766	arg1	SS					1771:1772	SS	1771:1772	SS	1771:1772	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	6	8	theme	bacteria	1113:1120	arg1	counts					1073:1078	counts	1073:1078	counts of lactic bacteria and anaerobic bacteria	1073:1120	The CHI and INO alone increased counts of lactic bacteria and anaerobic bacteria and decreased counts of mold and yeast in SS.					
29935832	10	9	theme	losses	1570:1575	arg1	value					1557:1561	the lowest value	1546:1561	the lowest value of gas losses	1546:1575	The combination of CHI+INO showed the lowest value of gas losses.					
29935832	4	10	dep	g/t	746:748	arg1	forage					756:761	forage	756:761	forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI	756:895	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	4	11	theme	Kera	792:795	arg1	SIL					797:799	Kera SIL	792:799	Kera SIL	792:799	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	7	12	theme	crude	1235:1239	arg1	protein					1241:1247	crude protein	1235:1247	crude protein	1235:1247	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	11	13	theme	INO	1617:1619	arg1	combination					1594:1604	the combination	1590:1604	the combination of CHI and INO	1590:1619	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	3	14	theme	total	555:559	arg1	losses					561:566	total losses	555:566	total losses	555:566	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	7	15	theme	matter	1227:1232	arg1	degradation					1208:1218	in vitro degradation	1199:1218	in vitro degradation of dry matter, crude protein, and neutral detergent fiber	1199:1276	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	11	16	theme	CHI	1609:1611	arg1	combination					1594:1604	the combination	1590:1604	the combination of CHI and INO	1590:1619	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	1	17	theme	present	190:196	arg1	deacetylation					175:187	chitin deacetylation	168:187	chitin deacetylation	168:187	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	6	18	theme	bacteria	1090:1097	arg1	counts					1073:1078	counts	1073:1078	counts of lactic bacteria and anaerobic bacteria	1073:1120	The CHI and INO alone increased counts of lactic bacteria and anaerobic bacteria and decreased counts of mold and yeast in SS.					
29935832	11	19	from	mold	1753:1756	arg1	SS					1771:1772	SS	1771:1772	SS	1771:1772	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	3	20	theme	plant	585:589	arg1	SS					599:600	SS	599:600	SS	599:600	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	20	theme	plant	585:589	arg1	silage					591:596	whole-soybean plant silage	571:596	whole-soybean plant silage (SS) harvested at R6 stage	571:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	7	21	dep	in	1199:1200	arg1	vitro					1202:1206	vitro	1202:1206	vitro	1202:1206	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	4	22	dep	chitosan	873:880	arg1	forage					866:871	forage	866:871	forage	866:871	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	11	23	theme	small	1625:1629	arg1	effects					1640:1646	small positive effects	1625:1646	small positive effects	1625:1646	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	4	24	theme	chitosan	873:880	arg1	CHI					893:895	CHI	893:895	CHI	893:895	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	4	24	theme	chitosan	873:880	arg1	g/kg					852:855	5 g/kg	850:855	5 g/kg of fresh forage chitosan (CHI)	850:886	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	4	24	theme	chitosan	873:880	arg1	inoculant					776:784	microbial inoculant	766:784	microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil)	766:847	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	11	25	theme	in	1718:1719	arg1	degradation					1727:1737	nutrient in vitro degradation	1709:1737	nutrient in vitro degradation	1709:1737	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	4	26	theme	factorial	647:655	arg1	arrangement					657:667	a factorial arrangement	645:667	a factorial arrangement	645:667	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	3	27	from	degradation	516:526	arg1	effects					398:404	the effects	394:404	the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage	394:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	0	28	theme	Microorganism	68:80	arg1	counts					82:87	Microorganism counts	68:87	Microorganism counts	68:87	Soybean whole-plant ensiled with chitosan and lactic acid bacteria: Microorganism counts, fermentative profile, and total losses.					
29935832	5	29	theme	×	969:969	arg1	cfu/g					976:980	4.0 × 1010 cfu/g	965:980	4.0 × 1010 cfu/g	965:980	Microbial inoculant was composed of Lactobacillus plantarum (4.0 × 1010 cfu/g) and Propionibacterium acidipropionici (2.6 × 1010 cfu/g).					
29935832	5	29	theme	×	969:969	arg1	plantarum					954:962	Lactobacillus plantarum	940:962	Lactobacillus plantarum (4.0 × 1010 cfu/g)	940:981	Microbial inoculant was composed of Lactobacillus plantarum (4.0 × 1010 cfu/g) and Propionibacterium acidipropionici (2.6 × 1010 cfu/g).					
29935832	9	30	from	SS	1468:1469	arg1	recovery					1454:1461	dry matter recovery	1443:1461	dry matter recovery from SS	1443:1469	The CHI increased dry matter recovery from SS; INO increased silage aerobic stability.					
29935832	4	31	theme	fresh	860:864	arg1	chitosan					873:880	fresh forage chitosan	860:880	fresh forage chitosan (CHI)	860:886	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	4	31	theme	fresh	860:864	arg1	CHI					883:885	CHI	883:885	CHI	883:885	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	3	32	theme	chitosan	414:421	arg1	effects					398:404	the effects	394:404	the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage	394:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	33	theme	study	371:375	arg1	objective					353:361	The objective	349:361	The objective of this study	349:375	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	8	34	theme	lactate	1359:1365	arg1	concentrations					1367:1380	lactate concentrations	1359:1380	lactate concentrations	1359:1380	Chitosan increased NH3-N and lactate concentrations and decreased ethanol concentration in SS.					
29935832	0	35	theme	Soybean	0:6	arg1	whole-plant					8:18	Soybean whole-plant	0:18	Soybean whole-plant	0:18	Soybean whole-plant ensiled with chitosan and lactic acid bacteria: Microorganism counts, fermentative profile, and total losses.					
29935832	3	36	theme	additive	439:446	arg1	effects					398:404	the effects	394:404	the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage	394:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	37	dep	in	507:508	arg1	vitro					510:514	vitro	510:514	vitro	510:514	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	8	38	theme	ethanol	1396:1402	arg1	concentration					1404:1416	ethanol concentration	1396:1416	ethanol concentration in SS	1396:1422	Chitosan increased NH3-N and lactate concentrations and decreased ethanol concentration in SS.					
29935832	12	39	dep	Chitosan	1775:1782	arg1	utilization					1791:1801	utilization	1791:1801	utilization	1791:1801	Chitosan or INO utilization improves SS quality.					
29935832	1	40	attach	derived	155:161	arg2	biopolymer					144:153	a biopolymer	142:153	a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects	142:242	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	1	40	attach	derived	155:161	arg1	deacetylation					175:187	chitin deacetylation	168:187	chitin deacetylation	168:187	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	1	40	attach	derived	155:161	arg2	Chitosan					130:137	Chitosan	130:137	Chitosan	130:137	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	2	41	theme	antimicrobial	323:335	arg1	properties					337:346	its antimicrobial properties	319:346	its antimicrobial properties	319:346	Chitosan has been evaluated as rumen modulator and silage additive due to its antimicrobial properties.					
29935832	0	42	theme	lactic	46:51	arg1	bacteria					58:65	lactic acid bacteria	46:65	lactic acid bacteria	46:65	Soybean whole-plant ensiled with chitosan and lactic acid bacteria: Microorganism counts, fermentative profile, and total losses.					
29935832	5	43	theme	Microbial	904:912	arg1	inoculant					914:922	Microbial inoculant	904:922	Microbial inoculant	904:922	Microbial inoculant was composed of Lactobacillus plantarum (4.0 × 1010 cfu/g) and Propionibacterium acidipropionici (2.6 × 1010 cfu/g).					
29935832	11	44	theme	gas	1651:1653	arg1	losses					1655:1660	gas losses	1651:1660	gas losses of SS	1651:1666	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	6	45	from	counts	1136:1141	arg1	SS					1164:1165	SS	1164:1165	SS	1164:1165	The CHI and INO alone increased counts of lactic bacteria and anaerobic bacteria and decreased counts of mold and yeast in SS.					
29935832	7	46	theme	SS	1326:1327	arg1	content					1315:1321	nonfiber carbohydrate content	1293:1321	nonfiber carbohydrate content of SS	1293:1327	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	4	47	theme	fresh	750:754	arg1	g/t					746:748	8 g/t fresh	744:754	8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI	744:895	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	4	47	theme	fresh	750:754	arg1	additives					727:735	no additives	724:735	no additives (CON)	724:741	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	10	48	theme	gas	1566:1568	arg1	losses					1570:1575	gas losses	1566:1575	gas losses	1566:1575	The combination of CHI+INO showed the lowest value of gas losses.					
29935832	1	49	theme	crustaceans	220:230	arg1	exoskeleton					205:215	the exoskeleton	201:215	the exoskeleton of crustaceans and insects	201:242	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	1	50	from	exoskeleton	205:215	arg1	present					190:196	present	190:196	present	190:196	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	11	51	contain	had	1621:1623	arg2	effects					1640:1646	small positive effects	1625:1646	small positive effects	1625:1646	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	11	51	contain	had	1621:1623	arg1	combination					1594:1604	the combination	1590:1604	the combination of CHI and INO	1590:1619	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	6	52	theme	mold	1146:1149	arg1	counts					1136:1141	counts	1136:1141	counts of mold and yeast in SS	1136:1165	The CHI and INO alone increased counts of lactic bacteria and anaerobic bacteria and decreased counts of mold and yeast in SS.					
29935832	1	53	theme	insects	236:242	arg1	exoskeleton					205:215	the exoskeleton	201:215	the exoskeleton of crustaceans and insects	201:242	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	12	54	theme	SS	1812:1813	arg1	quality					1815:1821	SS quality	1812:1821	SS quality	1812:1821	Chitosan or INO utilization improves SS quality.					
29935832	7	55	theme	nonfiber	1293:1300	arg1	content					1315:1321	nonfiber carbohydrate content	1293:1321	nonfiber carbohydrate content of SS	1293:1327	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	2	56	theme	rumen	276:280	arg1	Chitosan					245:252	Chitosan	245:252	Chitosan	245:252	Chitosan has been evaluated as rumen modulator and silage additive due to its antimicrobial properties.					
29935832	2	56	theme	rumen	276:280	arg1	modulator					282:290	rumen modulator	276:290	rumen modulator	276:290	Chitosan has been evaluated as rumen modulator and silage additive due to its antimicrobial properties.					
29935832	9	57	theme	aerobic	1493:1499	arg1	stability					1501:1509	silage aerobic stability	1486:1509	silage aerobic stability	1486:1509	The CHI increased dry matter recovery from SS; INO increased silage aerobic stability.					
29935832	7	58	theme	detergent	1262:1270	arg1	fiber					1272:1276	neutral detergent fiber	1254:1276	neutral detergent fiber	1254:1276	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	4	59	from	treatments	631:640	arg1	arrangement					657:667	a factorial arrangement	645:667	a factorial arrangement	645:667	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	7	60	theme	fiber	1272:1276	arg1	degradation					1208:1218	in vitro degradation	1199:1218	in vitro degradation of dry matter, crude protein, and neutral detergent fiber	1199:1276	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	6	61	theme	anaerobic	1103:1111	arg1	bacteria					1113:1120	anaerobic bacteria	1103:1120	anaerobic bacteria	1103:1120	The CHI and INO alone increased counts of lactic bacteria and anaerobic bacteria and decreased counts of mold and yeast in SS.					
29935832	3	62	from	quality	467:473	arg1	effects					398:404	the effects	394:404	the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage	394:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	2	63	theme	additive	303:310	arg1	Chitosan					245:252	Chitosan	245:252	Chitosan	245:252	Chitosan has been evaluated as rumen modulator and silage additive due to its antimicrobial properties.					
29935832	2	63	theme	additive	303:310	arg1	modulator					282:290	rumen modulator	276:290	rumen modulator	276:290	Chitosan has been evaluated as rumen modulator and silage additive due to its antimicrobial properties.					
29935832	1	64	theme	chitin	168:173	arg1	deacetylation					175:187	chitin deacetylation	168:187	chitin deacetylation	168:187	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	7	65	theme	protein	1241:1247	arg1	degradation					1208:1218	in vitro degradation	1199:1218	in vitro degradation of dry matter, crude protein, and neutral detergent fiber	1199:1276	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	0	66	theme	total	116:120	arg1	losses					122:127	total losses	116:127	total losses	116:127	Soybean whole-plant ensiled with chitosan and lactic acid bacteria: Microorganism counts, fermentative profile, and total losses.					
29935832	9	67	theme	matter	1447:1452	arg1	recovery					1454:1461	dry matter recovery	1443:1461	dry matter recovery from SS	1443:1469	The CHI increased dry matter recovery from SS; INO increased silage aerobic stability.					
29935832	4	68	dep	inoculant	776:784	arg1	Brazil					841:846	Brazil	841:846	Brazil	841:846	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	4	68	dep	inoculant	776:784	arg1	INO					787:789	INO	787:789	INO	787:789	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	11	69	theme	SS	1665:1666	arg1	losses					1655:1660	gas losses	1651:1660	gas losses of SS	1651:1666	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	3	70	theme	whole-soybean	571:583	arg1	SS					599:600	SS	599:600	SS	599:600	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	70	theme	whole-soybean	571:583	arg1	silage					591:596	whole-soybean plant silage	571:596	whole-soybean plant silage (SS) harvested at R6 stage	571:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	7	71	theme	dry	1223:1225	arg1	matter					1227:1232	dry matter	1223:1232	dry matter	1223:1232	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	3	72	from	profile	542:548	arg1	effects					398:404	the effects	394:404	the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage	394:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	11	73	theme	positive	1631:1638	arg1	effects					1640:1646	small positive effects	1625:1646	small positive effects	1625:1646	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	6	74	theme	yeast	1155:1159	arg1	counts					1136:1141	counts	1136:1141	counts of mold and yeast in SS	1136:1165	The CHI and INO alone increased counts of lactic bacteria and anaerobic bacteria and decreased counts of mold and yeast in SS.					
29935832	1	75	from	present	190:196	arg1	exoskeleton					205:215	the exoskeleton	201:215	the exoskeleton of crustaceans and insects	201:242	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	7	76	theme	in	1199:1200	arg1	degradation					1208:1218	in vitro degradation	1199:1218	in vitro degradation of dry matter, crude protein, and neutral detergent fiber	1199:1276	The CHI or INO alone increased in vitro degradation of dry matter, crude protein, and neutral detergent fiber, and decreased nonfiber carbohydrate content of SS.					
29935832	3	77	theme	microbiological	451:465	arg1	quality					467:473	microbiological quality	451:473	microbiological quality	451:473	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	78	theme	silage	591:596	arg1	losses					561:566	total losses	555:566	total losses	555:566	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	78	theme	silage	591:596	arg1	composition					485:495	chemical composition	476:495	chemical composition	476:495	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	78	theme	silage	591:596	arg1	quality					467:473	microbiological quality	451:473	microbiological quality	451:473	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	78	theme	silage	591:596	arg1	profile					542:548	fermentative profile	529:548	fermentative profile	529:548	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	78	theme	silage	591:596	arg1	degradation					516:526	nutrient in vitro degradation	498:526	nutrient in vitro degradation	498:526	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	10	79	theme	CHI+INO	1531:1537	arg1	combination					1516:1526	The combination	1512:1526	The combination of CHI+INO	1512:1537	The combination of CHI+INO showed the lowest value of gas losses.					
29935832	0	80	theme	fermentative	90:101	arg1	profile					103:109	fermentative profile	90:109	fermentative profile	90:109	Soybean whole-plant ensiled with chitosan and lactic acid bacteria: Microorganism counts, fermentative profile, and total losses.					
29935832	3	81	from	effects	398:404	arg1	profile					542:548	fermentative profile	529:548	fermentative profile	529:548	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	81	from	effects	398:404	arg1	losses					561:566	total losses	555:566	total losses	555:566	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	81	from	effects	398:404	arg1	quality					467:473	microbiological quality	451:473	microbiological quality	451:473	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	81	from	effects	398:404	arg1	composition					485:495	chemical composition	476:495	chemical composition	476:495	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	81	from	effects	398:404	arg1	degradation					516:526	nutrient in vitro degradation	498:526	nutrient in vitro degradation	498:526	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	4	82	dep	Brazil	841:846	arg1	Bento					824:828	Bento	824:828	Bento	824:828	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	4	83	theme	experimental	698:709	arg1	minisilos					711:719	40 experimental minisilos	695:719	40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO	695:901	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	3	84	from	losses	561:566	arg1	effects					398:404	the effects	394:404	the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage	394:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	4	85	dep	INO	787:789	arg1	SIL					797:799	Kera SIL	792:799	Kera SIL	792:799	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	3	86	theme	R6	616:617	arg1	stage					619:623	R6 stage	616:623	R6 stage	616:623	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	3	87	theme	nutrient	498:505	arg1	degradation					516:526	nutrient in vitro degradation	498:526	nutrient in vitro degradation	498:526	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	11	88	theme	nutrient	1709:1716	arg1	degradation					1727:1737	nutrient in vitro degradation	1709:1737	nutrient in vitro degradation	1709:1737	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	0	89	theme	acid	53:56	arg1	bacteria					58:65	lactic acid bacteria	46:65	lactic acid bacteria	46:65	Soybean whole-plant ensiled with chitosan and lactic acid bacteria: Microorganism counts, fermentative profile, and total losses.					
29935832	5	90	theme	×	1026:1026	arg1	cfu/g					1033:1037	2.6 × 1010 cfu/g	1022:1037	2.6 × 1010 cfu/g	1022:1037	Microbial inoculant was composed of Lactobacillus plantarum (4.0 × 1010 cfu/g) and Propionibacterium acidipropionici (2.6 × 1010 cfu/g).					
29935832	5	90	theme	×	1026:1026	arg1	acidipropionici					1005:1019	Propionibacterium acidipropionici	987:1019	Propionibacterium acidipropionici (2.6 × 1010 cfu/g)	987:1038	Microbial inoculant was composed of Lactobacillus plantarum (4.0 × 1010 cfu/g) and Propionibacterium acidipropionici (2.6 × 1010 cfu/g).					
29935832	9	91	theme	dry	1443:1445	arg1	recovery					1454:1461	dry matter recovery	1443:1461	dry matter recovery from SS	1443:1469	The CHI increased dry matter recovery from SS; INO increased silage aerobic stability.					
29935832	1	92	attach	present	190:196	arg1	exoskeleton					205:215	the exoskeleton	201:215	the exoskeleton of crustaceans and insects	201:242	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	1	92	attach	present	190:196	arg2	deacetylation					175:187	chitin deacetylation	168:187	chitin deacetylation	168:187	Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects.					
29935832	4	93	theme	microbial	766:774	arg1	inoculant					776:784	microbial inoculant	766:784	microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil)	766:847	Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO.					
29935832	3	94	theme	chemical	476:483	arg1	composition					485:495	chemical composition	476:495	chemical composition	476:495	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29935832	6	95	theme	lactic	1083:1088	arg1	bacteria					1090:1097	lactic bacteria	1083:1097	lactic bacteria	1083:1097	The CHI and INO alone increased counts of lactic bacteria and anaerobic bacteria and decreased counts of mold and yeast in SS.					
29935832	11	96	dep	in	1718:1719	arg1	vitro					1721:1725	vitro	1721:1725	vitro	1721:1725	In general, the combination of CHI and INO had small positive effects on gas losses of SS; however, both CHI or INO alone improved nutrient in vitro degradation and decreased mold and yeast in SS.					
29935832	3	97	theme	in	507:508	arg1	degradation					516:526	nutrient in vitro degradation	498:526	nutrient in vitro degradation	498:526	The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage.					
29359866	10	0	theme	novel	1533:1537	arg1	potential					1497:1505	the potential	1493:1505	the potential of CA/SPH nanofibers	1493:1526	These results affirm the potential of CA/SPH nanofibers as a novel wound dressing.					
29359866	10	0	theme	novel	1533:1537	arg1	dressing					1545:1552	a novel wound dressing	1531:1552	a novel wound dressing	1531:1552	These results affirm the potential of CA/SPH nanofibers as a novel wound dressing.					
29359866	6	1	theme	protein	906:912	arg1	SPH					927:929	SPH	927:929	SPH	927:929	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	6	1	theme	protein	906:912	arg1	hydrolysate					914:924	soy protein hydrolysate	902:924	soy protein hydrolysate (SPH)	902:930	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	2	2	theme	skin	345:348	arg1	regeneration					350:361	enhanced skin regeneration	336:361	enhanced skin regeneration	336:361	More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration.					
29359866	0	3	theme	Wound	91:95	arg1	Healing					97:103	Enhanced Wound Healing	82:103	Enhanced Wound Healing	82:103	Soy Protein/Cellulose Nanofiber Scaffolds Mimicking Skin Extracellular Matrix for Enhanced Wound Healing.					
29359866	6	4	theme	soy	902:904	arg1	SPH					927:929	SPH	927:929	SPH	927:929	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	6	4	theme	soy	902:904	arg1	hydrolysate					914:924	soy protein hydrolysate	902:924	soy protein hydrolysate (SPH)	902:930	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	2	5	theme	enhanced	336:343	arg1	regeneration					350:361	enhanced skin regeneration	336:361	enhanced skin regeneration	336:361	More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration.					
29359866	8	6	theme	CA/SPH	1118:1123	arg1	nanofibers					1125:1134	CA/SPH nanofibers	1118:1134	CA/SPH nanofibers	1118:1134	In vitro, CA/SPH nanofibers promote fibroblast proliferation, migration, infiltration, and integrin β1 expression.					
29359866	4	7	theme	wound	496:500	arg1	healing					502:508	wound healing	496:508	wound healing	496:508	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	9	8	dep	scaffolds	1239:1247	arg1	accelerate					1249:1258	accelerate	1249:1258	scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers	1239:1469	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	9	8	dep	scaffolds	1239:1247	arg1	reduce					1315:1320	reduce	1315:1320	scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers	1239:1469	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	5	9	dep	dressings	681:689	arg1	maintaining					738:748	maintaining	738:748	maintaining a moist environment	738:768	However, engineering soy protein-based fibrous dressings, while recapitulating ECM microenvironment and maintaining a moist environment, remains a challenge.					
29359866	5	9	dep	dressings	681:689	arg1	recapitulating					698:711	recapitulating	698:711	recapitulating ECM microenvironment	698:732	However, engineering soy protein-based fibrous dressings, while recapitulating ECM microenvironment and maintaining a moist environment, remains a challenge.					
29359866	5	10	theme	engineering	643:653	arg1	dressings					681:689	engineering soy protein-based fibrous dressings	643:689	engineering soy protein-based fibrous dressings	643:689	However, engineering soy protein-based fibrous dressings, while recapitulating ECM microenvironment and maintaining a moist environment, remains a challenge.					
29359866	3	11	contain	possesses	402:410	arg1	protein					394:400	soy protein	390:400	soy protein	390:400	It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen.					
29359866	3	11	contain	possesses	402:410	arg2	molecules					422:430	bioactive molecules	412:430	bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen	412:490	It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen.					
29359866	9	12	theme	synthetic	1452:1460	arg1	polymers					1462:1469	synthetic polymers	1452:1469	synthetic polymers	1452:1469	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	4	13	theme	safe	555:558	arg1	alternative					579:589	a safe and cost-effective alternative	553:589	a safe and cost-effective alternative to animal protein and endogenous estrogen	553:631	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	4	13	theme	safe	555:558	arg1	soy					528:530	oral and topical soy	511:530	oral and topical soy	511:530	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	9	14	theme	polymers	1462:1469	arg1	use					1426:1428	the use	1422:1428	the use of animal proteins or synthetic polymers	1422:1469	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	1	15	used	used	155:158	arg2	protein					124:130	soy protein	120:130	soy protein	120:130	Historically, soy protein and extracts have been used extensively in foods due to their high protein and mineral content.					
29359866	1	15	used	used	155:158	arg2	extracts					136:143	extracts	136:143	extracts	136:143	Historically, soy protein and extracts have been used extensively in foods due to their high protein and mineral content.					
29359866	9	16	theme	proteins	1440:1447	arg1	use					1426:1428	the use	1422:1428	the use of animal proteins or synthetic polymers	1422:1469	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	3	17	theme	similar	432:438	arg1	molecules					422:430	bioactive molecules	412:430	bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen	412:490	It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen.					
29359866	0	18	theme	Extracellular	57:69	arg1	Matrix					71:76	Skin Extracellular Matrix	52:76	Skin Extracellular Matrix for Enhanced Wound Healing	52:103	Soy Protein/Cellulose Nanofiber Scaffolds Mimicking Skin Extracellular Matrix for Enhanced Wound Healing.					
29359866	9	19	theme	scar	1322:1325	arg1	formation					1327:1335	scar formation	1322:1335	scar formation	1322:1335	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	8	20	theme	β1	1208:1209	arg1	expression					1211:1220	integrin β1 expression	1199:1220	integrin β1 expression	1199:1220	In vitro, CA/SPH nanofibers promote fibroblast proliferation, migration, infiltration, and integrin β1 expression.					
29359866	9	21	theme	fibrous	1391:1397	arg1	scaffolds					1399:1407	other fibrous scaffolds	1385:1407	other fibrous scaffolds	1385:1407	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	1	22	theme	due	181:183	arg1	foods					175:179	foods	175:179	foods due to their high protein and mineral content	175:225	Historically, soy protein and extracts have been used extensively in foods due to their high protein and mineral content.					
29359866	0	23	theme	Protein/Cellulose	4:20	arg1	Scaffolds					32:40	Soy Protein/Cellulose Nanofiber Scaffolds	0:40	Soy Protein/Cellulose Nanofiber Scaffolds	0:40	Soy Protein/Cellulose Nanofiber Scaffolds Mimicking Skin Extracellular Matrix for Enhanced Wound Healing.					
29359866	5	24	theme	moist	752:756	arg1	environment					758:768	a moist environment	750:768	a moist environment	750:768	However, engineering soy protein-based fibrous dressings, while recapitulating ECM microenvironment and maintaining a moist environment, remains a challenge.					
29359866	2	25	theme	potential	299:307	arg1	benefits					316:323	its potential health benefits	295:323	its potential health benefits	295:323	More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration.					
29359866	7	26	theme	native	1045:1050	arg1	ECM					1057:1059	the native skin ECM	1041:1059	the native skin ECM	1041:1059	The spun nanofibers successfully mimic physicochemical properties of the native skin ECM and exhibit a high water retaining capability.					
29359866	3	27	theme	bioactive	412:420	arg1	molecules					422:430	bioactive molecules	412:430	bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen	412:490	It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen.					
29359866	0	28	theme	Soy	0:2	arg1	Scaffolds					32:40	Soy Protein/Cellulose Nanofiber Scaffolds	0:40	Soy Protein/Cellulose Nanofiber Scaffolds	0:40	Soy Protein/Cellulose Nanofiber Scaffolds Mimicking Skin Extracellular Matrix for Enhanced Wound Healing.					
29359866	7	29	theme	high	1075:1078	arg1	water					1080:1084	a high water	1073:1084	a high water retaining capability	1073:1105	The spun nanofibers successfully mimic physicochemical properties of the native skin ECM and exhibit a high water retaining capability.					
29359866	10	30	theme	nanofibers	1517:1526	arg1	dressing					1545:1552	a novel wound dressing	1531:1552	a novel wound dressing	1531:1552	These results affirm the potential of CA/SPH nanofibers as a novel wound dressing.					
29359866	10	30	theme	nanofibers	1517:1526	arg1	potential					1497:1505	the potential	1493:1505	the potential of CA/SPH nanofibers	1493:1526	These results affirm the potential of CA/SPH nanofibers as a novel wound dressing.					
29359866	9	31	theme	collagen	1341:1348	arg1	anisotropy					1350:1359	collagen anisotropy	1341:1359	collagen anisotropy	1341:1359	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	1	32	theme	high	194:197	arg1	protein					199:205	their high protein	188:205	their high protein	188:205	Historically, soy protein and extracts have been used extensively in foods due to their high protein and mineral content.					
29359866	0	33	theme	Nanofiber	22:30	arg1	Scaffolds					32:40	Soy Protein/Cellulose Nanofiber Scaffolds	0:40	Soy Protein/Cellulose Nanofiber Scaffolds	0:40	Soy Protein/Cellulose Nanofiber Scaffolds Mimicking Skin Extracellular Matrix for Enhanced Wound Healing.					
29359866	6	34	theme	jet	945:947	arg1	spinning					949:956	rotary jet spinning	938:956	rotary jet spinning	938:956	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	6	35	theme	cellulose	875:883	arg1	CA					894:895	CA	894:895	CA	894:895	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	6	35	theme	cellulose	875:883	arg1	acetate					885:891	cellulose acetate	875:891	cellulose acetate (CA)	875:896	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	1	36	theme	soy	120:122	arg1	protein					124:130	soy protein	120:130	soy protein	120:130	Historically, soy protein and extracts have been used extensively in foods due to their high protein and mineral content.					
29359866	3	37	theme	ECM	465:467	arg1	proteins					470:477	extracellular matrix (ECM) proteins	443:477	extracellular matrix (ECM) proteins	443:477	It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen.					
29359866	0	38	theme	Skin	52:55	arg1	Matrix					71:76	Skin Extracellular Matrix	52:76	Skin Extracellular Matrix for Enhanced Wound Healing	52:103	Soy Protein/Cellulose Nanofiber Scaffolds Mimicking Skin Extracellular Matrix for Enhanced Wound Healing.					
29359866	6	39	theme	rotary	938:943	arg1	spinning					949:956	rotary jet spinning	938:956	rotary jet spinning	938:956	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	7	40	theme	skin	1052:1055	arg1	ECM					1057:1059	the native skin ECM	1041:1059	the native skin ECM	1041:1059	The spun nanofibers successfully mimic physicochemical properties of the native skin ECM and exhibit a high water retaining capability.					
29359866	4	41	theme	endogenous	613:622	arg1	estrogen					624:631	endogenous estrogen	613:631	endogenous estrogen	613:631	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	3	42	theme	extracellular	443:455	arg1	proteins					470:477	extracellular matrix (ECM) proteins	443:477	extracellular matrix (ECM) proteins	443:477	It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen.					
29359866	8	43	theme	integrin	1199:1206	arg1	expression					1211:1220	integrin β1 expression	1199:1220	integrin β1 expression	1199:1220	In vitro, CA/SPH nanofibers promote fibroblast proliferation, migration, infiltration, and integrin β1 expression.					
29359866	4	44	theme	oral	511:514	arg1	alternative					579:589	a safe and cost-effective alternative	553:589	a safe and cost-effective alternative to animal protein and endogenous estrogen	553:631	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	4	44	theme	oral	511:514	arg1	soy					528:530	oral and topical soy	511:530	oral and topical soy	511:530	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	5	45	theme	ECM	713:715	arg1	microenvironment					717:732	ECM microenvironment	713:732	ECM microenvironment	713:732	However, engineering soy protein-based fibrous dressings, while recapitulating ECM microenvironment and maintaining a moist environment, remains a challenge.					
29359866	1	46	theme	mineral	211:217	arg1	content					219:225	mineral content	211:225	mineral content	211:225	Historically, soy protein and extracts have been used extensively in foods due to their high protein and mineral content.					
29359866	10	47	theme	CA/SPH	1510:1515	arg1	nanofibers					1517:1526	CA/SPH nanofibers	1510:1526	CA/SPH nanofibers	1510:1526	These results affirm the potential of CA/SPH nanofibers as a novel wound dressing.					
29359866	9	48	theme	similar	1366:1372	arg1	fashion					1374:1380	a similar fashion	1364:1380	a similar fashion to other fibrous scaffolds	1364:1407	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	5	49	theme	soy	655:657	arg1	dressings					681:689	engineering soy protein-based fibrous dressings	643:689	engineering soy protein-based fibrous dressings	643:689	However, engineering soy protein-based fibrous dressings, while recapitulating ECM microenvironment and maintaining a moist environment, remains a challenge.					
29359866	6	50	theme	dressing	853:860	arg1	development					802:812	the development	798:812	the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning	798:956	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	7	51	theme	physicochemical	1011:1025	arg1	properties					1027:1036	physicochemical properties	1011:1036	physicochemical properties of the native skin ECM	1011:1059	The spun nanofibers successfully mimic physicochemical properties of the native skin ECM and exhibit a high water retaining capability.					
29359866	7	52	theme	ECM	1057:1059	arg1	properties					1027:1036	physicochemical properties	1011:1036	physicochemical properties of the native skin ECM	1011:1059	The spun nanofibers successfully mimic physicochemical properties of the native skin ECM and exhibit a high water retaining capability.					
29359866	3	53	theme	soy	390:392	arg1	protein					394:400	soy protein	390:400	soy protein	390:400	It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen.					
29359866	4	54	theme	topical	520:526	arg1	alternative					579:589	a safe and cost-effective alternative	553:589	a safe and cost-effective alternative to animal protein and endogenous estrogen	553:631	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	4	54	theme	topical	520:526	arg1	soy					528:530	oral and topical soy	511:530	oral and topical soy	511:530	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	5	55	theme	protein-based	659:671	arg1	dressings					681:689	engineering soy protein-based fibrous dressings	643:689	engineering soy protein-based fibrous dressings	643:689	However, engineering soy protein-based fibrous dressings, while recapitulating ECM microenvironment and maintaining a moist environment, remains a challenge.					
29359866	6	56	theme	nanofibrous	841:851	arg1	dressing					853:860	an entirely plant-based nanofibrous dressing	817:860	an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning	817:956	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	5	57	theme	fibrous	673:679	arg1	dressings					681:689	engineering soy protein-based fibrous dressings	643:689	engineering soy protein-based fibrous dressings	643:689	However, engineering soy protein-based fibrous dressings, while recapitulating ECM microenvironment and maintaining a moist environment, remains a challenge.					
29359866	6	58	theme	plant-based	829:839	arg1	dressing					853:860	an entirely plant-based nanofibrous dressing	817:860	an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning	817:956	Here, the development of an entirely plant-based nanofibrous dressing comprised of cellulose acetate (CA) and soy protein hydrolysate (SPH) using rotary jet spinning is described.					
29359866	2	59	theme	benefits	316:323	arg1	regeneration					350:361	enhanced skin regeneration	336:361	enhanced skin regeneration	336:361	More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration.					
29359866	2	59	theme	benefits	316:323	arg1	benefits					316:323	its potential health benefits	295:323	its potential health benefits	295:323	More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration.					
29359866	2	59	theme	benefits	316:323	arg1	variety					284:290	a variety	282:290	a variety	282:290	More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration.					
29359866	9	60	theme	other	1385:1389	arg1	scaffolds					1399:1407	other fibrous scaffolds	1385:1407	other fibrous scaffolds	1385:1407	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	2	61	theme	health	309:314	arg1	benefits					316:323	its potential health benefits	295:323	its potential health benefits	295:323	More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration.					
29359866	4	62	theme	cost-effective	564:577	arg1	alternative					579:589	a safe and cost-effective alternative	553:589	a safe and cost-effective alternative to animal protein and endogenous estrogen	553:631	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	4	62	theme	cost-effective	564:577	arg1	soy					528:530	oral and topical soy	511:530	oral and topical soy	511:530	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	0	63	theme	Enhanced	82:89	arg1	Healing					97:103	Enhanced Wound Healing	82:103	Enhanced Wound Healing	82:103	Soy Protein/Cellulose Nanofiber Scaffolds Mimicking Skin Extracellular Matrix for Enhanced Wound Healing.					
29359866	3	64	theme	matrix	457:462	arg1	proteins					470:477	extracellular matrix (ECM) proteins	443:477	extracellular matrix (ECM) proteins	443:477	It has been reported that soy protein possesses bioactive molecules similar to extracellular matrix (ECM) proteins and estrogen.					
29359866	2	65	theme	soy	243:245	arg1	protein					247:253	soy protein	243:253	soy protein	243:253	More recently, soy protein has received attention for a variety of its potential health benefits, including enhanced skin regeneration.					
29359866	7	66	theme	spun	976:979	arg1	nanofibers					981:990	The spun nanofibers	972:990	The spun nanofibers	972:990	The spun nanofibers successfully mimic physicochemical properties of the native skin ECM and exhibit a high water retaining capability.					
29359866	9	67	theme	animal	1433:1438	arg1	proteins					1440:1447	animal proteins	1433:1447	animal proteins	1433:1447	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	4	68	theme	animal	594:599	arg1	protein					601:607	animal protein	594:607	animal protein	594:607	In wound healing, oral and topical soy has been heralded as a safe and cost-effective alternative to animal protein and endogenous estrogen.					
29359866	10	69	theme	wound	1539:1543	arg1	potential					1497:1505	the potential	1493:1505	the potential of CA/SPH nanofibers	1493:1526	These results affirm the potential of CA/SPH nanofibers as a novel wound dressing.					
29359866	10	69	theme	wound	1539:1543	arg1	dressing					1545:1552	a novel wound dressing	1531:1552	a novel wound dressing	1531:1552	These results affirm the potential of CA/SPH nanofibers as a novel wound dressing.					
29359866	9	70	theme	epidermal	1285:1293	arg1	thinning					1295:1302	epidermal thinning	1285:1302	epidermal thinning	1285:1302	In vivo, CA/SPH scaffolds accelerate re-epithelialization and epidermal thinning as well as reduce scar formation and collagen anisotropy in a similar fashion to other fibrous scaffolds, but without the use of animal proteins or synthetic polymers.					
29359866	8	71	theme	fibroblast	1144:1153	arg1	proliferation					1155:1167	fibroblast proliferation	1144:1167	fibroblast proliferation	1144:1167	In vitro, CA/SPH nanofibers promote fibroblast proliferation, migration, infiltration, and integrin β1 expression.					
31518870	6	0	from	wound	1324:1328	arg1	delivery					1285:1292	the controlled delivery	1270:1292	the controlled delivery of bioactive molecules on the wound and/or cancer	1270:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	4	1	theme	pHotoelectron	820:832	arg1	GC-MS					990:994	GC-MS	990:994	GC-MS	990:994	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	1	theme	pHotoelectron	820:832	arg1	XPS					848:850	XPS	848:850	XPS	848:850	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	1	theme	pHotoelectron	820:832	arg1	Spectroscopy					834:845	X-Ray pHotoelectron Spectroscopy	814:845	X-Ray pHotoelectron Spectroscopy (XPS)	814:851	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	1	theme	pHotoelectron	820:832	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	1	2	theme	Bio	144:146	arg1	BioMOF					173:178	BioMOF	173:178	BioMOF	173:178	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	1	2	theme	Bio	144:146	arg1	Framework					162:170	the zinc glutamate Bio Metal-Organic Framework	125:170	the zinc glutamate Bio Metal-Organic Framework (BioMOF)	125:179	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	5	3	theme	melanoma	1049:1056	arg1	cell					1063:1066	melanoma skin cell	1049:1066	melanoma skin cell	1049:1066	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	5	4	theme	BioMOF	1145:1150	arg1	activity					1117:1124	the biological activity	1102:1124	the biological activity of the drug-loaded BioMOF on treated cellulose fabrics	1102:1179	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	2	5	theme	NO	436:437	arg1	incorporation					419:431	the incorporation	415:431	the incorporation of NO and 5FU	415:445	In situ synthesis of BioMOF on the cotton fabric was successfully achieved and the incorporation of NO and 5FU was studied.					
31518870	1	6	theme	wound	302:306	arg1	therapy					327:333	wound and/or skin cancer therapy	302:333	therapy	327:333	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	4	7	theme	X-Ray	814:818	arg1	GC-MS					990:994	GC-MS	990:994	GC-MS	990:994	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	7	theme	X-Ray	814:818	arg1	XPS					848:850	XPS	848:850	XPS	848:850	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	7	theme	X-Ray	814:818	arg1	Spectroscopy					834:845	X-Ray pHotoelectron Spectroscopy	814:845	X-Ray pHotoelectron Spectroscopy (XPS)	814:851	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	7	theme	X-Ray	814:818	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	1	8	theme	Metal-Organic	148:160	arg1	BioMOF					173:178	BioMOF	173:178	BioMOF	173:178	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	1	8	theme	Metal-Organic	148:160	arg1	Framework					162:170	the zinc glutamate Bio Metal-Organic Framework	125:170	the zinc glutamate Bio Metal-Organic Framework (BioMOF)	125:179	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	1	9	theme	nitric	235:240	arg1	NO					249:250	NO	249:250	NO	249:250	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	1	9	theme	nitric	235:240	arg1	oxide					242:246	nitric oxide	235:246	nitric oxide (NO)	235:251	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	4	10	theme	N2	888:889	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	10	theme	N2	888:889	arg1	analysis					902:909	N2 adsorption analysis	888:909	N2 adsorption analysis	888:909	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	11	theme	Scanning	731:738	arg1	Microscopy					749:758	Field Emission Scanning Electron Microscopy	716:758	Field Emission Scanning Electron Microscopy (FESEM)	716:766	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	11	theme	Scanning	731:738	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	11	theme	Scanning	731:738	arg1	FESEM					761:765	FESEM	761:765	FESEM	761:765	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	12	theme	adsorption	891:900	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	12	theme	adsorption	891:900	arg1	analysis					902:909	N2 adsorption analysis	888:909	N2 adsorption analysis	888:909	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	1	13	theme	skin	315:318	arg1	therapy					327:333	wound and/or skin cancer therapy	302:333	therapy	327:333	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	6	14	theme	potential	1218:1226	arg1	indicative					1200:1209	indicative	1200:1209	indicative	1200:1209	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	5	15	theme	biological	1106:1115	arg1	activity					1117:1124	the biological activity	1102:1124	the biological activity of the drug-loaded BioMOF on treated cellulose fabrics	1102:1179	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	5	16	theme	cellulose	1163:1171	arg1	fabrics					1173:1179	treated cellulose fabrics	1155:1179	treated cellulose fabrics	1155:1179	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	6	17	theme	molecules	1307:1315	arg1	delivery					1285:1292	the controlled delivery	1270:1292	the controlled delivery of bioactive molecules on the wound and/or cancer	1270:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	1	18	theme	cancer	320:325	arg1	therapy					327:333	wound and/or skin cancer therapy	302:333	therapy	327:333	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	4	19	theme	UV-Vis-NIR	789:798	arg1	spectrometer					800:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	19	theme	UV-Vis-NIR	789:798	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	20	theme	Emission	722:729	arg1	Microscopy					749:758	Field Emission Scanning Electron Microscopy	716:758	Field Emission Scanning Electron Microscopy (FESEM)	716:766	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	20	theme	Emission	722:729	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	20	theme	Emission	722:729	arg1	FESEM					761:765	FESEM	761:765	FESEM	761:765	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	6	21	theme	@	1237:1237	arg1	composites					1255:1264	BioMOF@cellulose fabric composites	1231:1264	BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer	1231:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	1	22	theme	Framework	162:170	arg1	application					110:120	application	110:120	application of the zinc glutamate Bio Metal-Organic Framework (BioMOF)	110:179	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	1	22	theme	Framework	162:170	arg1	synthesis					96:104	situ synthesis	91:104	situ synthesis	91:104	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	4	23	theme	Gas	952:954	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	23	theme	Gas	952:954	arg1	Spectroscopy					976:987	Gas Chromatography-Mass Spectroscopy	952:987	Gas Chromatography-Mass Spectroscopy	952:987	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	24	theme	Reflectance	777:787	arg1	spectrometer					800:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	24	theme	Reflectance	777:787	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	6	25	from	delivery	1285:1292	arg1	cancer					1337:1342	the wound and/or cancer	1320:1342	cancer	1337:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	6	25	from	delivery	1285:1292	arg1	wound					1324:1328	the wound and/or cancer	1320:1342	wound	1324:1328	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	4	26	theme	Field	716:720	arg1	Microscopy					749:758	Field Emission Scanning Electron Microscopy	716:758	Field Emission Scanning Electron Microscopy (FESEM)	716:766	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	26	theme	Field	716:720	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	26	theme	Field	716:720	arg1	FESEM					761:765	FESEM	761:765	FESEM	761:765	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	6	27	from	cancer	1337:1342	arg1	delivery					1285:1292	the controlled delivery	1270:1292	the controlled delivery of bioactive molecules on the wound and/or cancer	1270:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	0	28	theme	@	6:6	arg1	cellulose					7:15	BioMOF@cellulose	0:15	BioMOF@cellulose	0:15	BioMOF@cellulose fabric composites for bioactive molecule delivery.					
31518870	4	29	theme	UV-Visible	923:932	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	29	theme	UV-Visible	923:932	arg1	spectroscopy					934:945	Transition UV-Visible spectroscopy	912:945	Transition UV-Visible spectroscopy	912:945	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	5	30	from	activity	1117:1124	arg1	fabrics					1173:1179	treated cellulose fabrics	1155:1179	treated cellulose fabrics	1155:1179	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	3	31	theme	metal	505:509	arg1	ions					511:514	FeII metal ions	500:514	FeII metal ions	500:514	We have observed that BioMOF doped with FeII metal ions has a beneficial impact on NO incorporation.					
31518870	5	32	theme	skin	1058:1061	arg1	cell					1063:1066	melanoma skin cell	1049:1066	melanoma skin cell	1049:1066	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	4	33	theme	powder	608:613	arg1	Microscopy					749:758	Field Emission Scanning Electron Microscopy	716:758	Field Emission Scanning Electron Microscopy (FESEM)	716:766	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	33	theme	powder	608:613	arg1	spectrometer					800:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	33	theme	powder	608:613	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	33	theme	powder	608:613	arg1	Spectroscopy					976:987	Gas Chromatography-Mass Spectroscopy	952:987	Gas Chromatography-Mass Spectroscopy	952:987	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	33	theme	powder	608:613	arg1	spectroscopy					934:945	Transition UV-Visible spectroscopy	912:945	Transition UV-Visible spectroscopy	912:945	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	33	theme	powder	608:613	arg1	XRPD					628:631	XRPD	628:631	XRPD	628:631	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	33	theme	powder	608:613	arg1	Reflectance					692:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance	635:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR)	635:713	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	33	theme	powder	608:613	arg1	analysis					902:909	N2 adsorption analysis	888:909	N2 adsorption analysis	888:909	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	33	theme	powder	608:613	arg1	Spectroscopy					834:845	X-Ray pHotoelectron Spectroscopy	814:845	X-Ray pHotoelectron Spectroscopy (XPS)	814:851	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	33	theme	powder	608:613	arg1	Analysis					872:879	Thermogravimetric Analysis	854:879	Thermogravimetric Analysis (TGA)	854:885	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	0	34	theme	BioMOF	0:5	arg1	cellulose					7:15	BioMOF@cellulose	0:15	BioMOF@cellulose	0:15	BioMOF@cellulose fabric composites for bioactive molecule delivery.					
31518870	4	35	theme	Chromatography-Mass	956:974	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	35	theme	Chromatography-Mass	956:974	arg1	Spectroscopy					976:987	Gas Chromatography-Mass Spectroscopy	952:987	Gas Chromatography-Mass Spectroscopy	952:987	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	36	theme	Total	686:690	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	36	theme	Total	686:690	arg1	FTIR-ATR					705:712	FTIR-ATR	705:712	FTIR-ATR	705:712	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	36	theme	Total	686:690	arg1	Reflectance					692:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance	635:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR)	635:713	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	2	37	theme	In	336:337	arg1	synthesis					344:352	In situ synthesis	336:352	In situ synthesis of BioMOF on the cotton fabric	336:383	In situ synthesis of BioMOF on the cotton fabric was successfully achieved and the incorporation of NO and 5FU was studied.					
31518870	6	38	theme	bioactive	1297:1305	arg1	molecules					1307:1315	bioactive molecules	1297:1315	bioactive molecules on the wound and/or cancer	1297:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	6	39	theme	BioMOF	1231:1236	arg1	composites					1255:1264	BioMOF@cellulose fabric composites	1231:1264	BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer	1231:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	1	40	theme	situ	91:94	arg1	synthesis					96:104	situ synthesis	91:104	situ synthesis	91:104	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	2	41	theme	cotton	371:376	arg1	fabric					378:383	the cotton fabric	367:383	the cotton fabric	367:383	In situ synthesis of BioMOF on the cotton fabric was successfully achieved and the incorporation of NO and 5FU was studied.					
31518870	5	42	dep	carried	1072:1078	arg1	out					1080:1082	out	1080:1082	out	1080:1082	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	3	43	theme	FeII	500:503	arg1	ions					511:514	FeII metal ions	500:514	FeII metal ions	500:514	We have observed that BioMOF doped with FeII metal ions has a beneficial impact on NO incorporation.					
31518870	5	44	theme	drug-loaded	1133:1143	arg1	BioMOF					1145:1150	the drug-loaded BioMOF	1129:1150	the drug-loaded BioMOF	1129:1150	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	6	45	theme	controlled	1274:1283	arg1	delivery					1285:1292	the controlled delivery	1270:1292	the controlled delivery of bioactive molecules on the wound and/or cancer	1270:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	5	46	theme	anticancer	1026:1035	arg1	activity					1037:1044	antibacterial and anticancer activity	1008:1044	antibacterial and anticancer activity in melanoma skin cell	1008:1066	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	3	47	theme	beneficial	522:531	arg1	impact					533:538	a beneficial impact	520:538	a beneficial impact	520:538	We have observed that BioMOF doped with FeII metal ions has a beneficial impact on NO incorporation.					
31518870	4	48	theme	X-ray	602:606	arg1	Microscopy					749:758	Field Emission Scanning Electron Microscopy	716:758	Field Emission Scanning Electron Microscopy (FESEM)	716:766	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	48	theme	X-ray	602:606	arg1	spectrometer					800:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	48	theme	X-ray	602:606	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	48	theme	X-ray	602:606	arg1	Spectroscopy					976:987	Gas Chromatography-Mass Spectroscopy	952:987	Gas Chromatography-Mass Spectroscopy	952:987	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	48	theme	X-ray	602:606	arg1	spectroscopy					934:945	Transition UV-Visible spectroscopy	912:945	Transition UV-Visible spectroscopy	912:945	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	48	theme	X-ray	602:606	arg1	XRPD					628:631	XRPD	628:631	XRPD	628:631	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	48	theme	X-ray	602:606	arg1	Reflectance					692:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance	635:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR)	635:713	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	48	theme	X-ray	602:606	arg1	analysis					902:909	N2 adsorption analysis	888:909	N2 adsorption analysis	888:909	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	48	theme	X-ray	602:606	arg1	Spectroscopy					834:845	X-Ray pHotoelectron Spectroscopy	814:845	X-Ray pHotoelectron Spectroscopy (XPS)	814:851	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	48	theme	X-ray	602:606	arg1	Analysis					872:879	Thermogravimetric Analysis	854:879	Thermogravimetric Analysis (TGA)	854:885	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	1	49	theme	cellulose	194:202	arg1	fabrics					204:210	cellulose fabrics	194:210	cellulose fabrics	194:210	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	3	50	contain	has	516:518	arg1	BioMOF					482:487	BioMOF	482:487	BioMOF doped with FeII metal ions	482:514	We have observed that BioMOF doped with FeII metal ions has a beneficial impact on NO incorporation.					
31518870	3	50	contain	has	516:518	arg2	impact					533:538	a beneficial impact	520:538	a beneficial impact	520:538	We have observed that BioMOF doped with FeII metal ions has a beneficial impact on NO incorporation.					
31518870	2	51	theme	BioMOF	357:362	arg1	synthesis					344:352	In situ synthesis	336:352	In situ synthesis of BioMOF on the cotton fabric	336:383	In situ synthesis of BioMOF on the cotton fabric was successfully achieved and the incorporation of NO and 5FU was studied.					
31518870	0	52	theme	molecule	49:56	arg1	delivery					58:65	bioactive molecule delivery	39:65	bioactive molecule delivery	39:65	BioMOF@cellulose fabric composites for bioactive molecule delivery.					
31518870	5	53	from	activity	1037:1044	arg1	cell					1063:1066	melanoma skin cell	1049:1066	melanoma skin cell	1049:1066	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	4	54	theme	Diffuse	769:775	arg1	spectrometer					800:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	Diffuse Reflectance UV-Vis-NIR spectrometer	769:811	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	54	theme	Diffuse	769:775	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	6	55	theme	fabric	1248:1253	arg1	composites					1255:1264	BioMOF@cellulose fabric composites	1231:1264	BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer	1231:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	4	56	theme	Fourier-Transform	635:651	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	56	theme	Fourier-Transform	635:651	arg1	FTIR-ATR					705:712	FTIR-ATR	705:712	FTIR-ATR	705:712	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	56	theme	Fourier-Transform	635:651	arg1	Reflectance					692:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance	635:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR)	635:713	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	0	57	theme	bioactive	39:47	arg1	delivery					58:65	bioactive molecule delivery	39:65	bioactive molecule delivery	39:65	BioMOF@cellulose fabric composites for bioactive molecule delivery.					
31518870	6	58	theme	composites	1255:1264	arg1	potential					1218:1226	the potential	1214:1226	the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer	1214:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	1	59	theme	controlled	278:287	arg1	delivery					289:296	controlled delivery	278:296	controlled delivery for wound and/or skin cancer therapy	278:333	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	4	60	theme	Thermogravimetric	854:870	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	60	theme	Thermogravimetric	854:870	arg1	TGA					882:884	TGA	882:884	TGA	882:884	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	60	theme	Thermogravimetric	854:870	arg1	Analysis					872:879	Thermogravimetric Analysis	854:879	Thermogravimetric Analysis (TGA)	854:885	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	61	theme	Electron	740:747	arg1	Microscopy					749:758	Field Emission Scanning Electron Microscopy	716:758	Field Emission Scanning Electron Microscopy (FESEM)	716:766	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	61	theme	Electron	740:747	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	61	theme	Electron	740:747	arg1	FESEM					761:765	FESEM	761:765	FESEM	761:765	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	2	62	from	synthesis	344:352	arg1	fabric					378:383	the cotton fabric	367:383	the cotton fabric	367:383	In situ synthesis of BioMOF on the cotton fabric was successfully achieved and the incorporation of NO and 5FU was studied.					
31518870	4	63	theme	Spectroscopy-Attenuated	662:684	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	63	theme	Spectroscopy-Attenuated	662:684	arg1	FTIR-ATR					705:712	FTIR-ATR	705:712	FTIR-ATR	705:712	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	63	theme	Spectroscopy-Attenuated	662:684	arg1	Reflectance					692:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance	635:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR)	635:713	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	6	64	theme	cellulose	1238:1246	arg1	composites					1255:1264	BioMOF@cellulose fabric composites	1231:1264	BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer	1231:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	2	65	dep	In	336:337	arg1	situ					339:342	situ	339:342	situ	339:342	In situ synthesis of BioMOF on the cotton fabric was successfully achieved and the incorporation of NO and 5FU was studied.					
31518870	6	66	from	molecules	1307:1315	arg1	cancer					1337:1342	the wound and/or cancer	1320:1342	cancer	1337:1342	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	6	66	from	molecules	1307:1315	arg1	wound					1324:1328	the wound and/or cancer	1320:1342	wound	1324:1328	These studies are indicative of the potential of BioMOF@cellulose fabric composites for the controlled delivery of bioactive molecules on the wound and/or cancer.					
31518870	4	67	theme	Infrared	653:660	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	67	theme	Infrared	653:660	arg1	FTIR-ATR					705:712	FTIR-ATR	705:712	FTIR-ATR	705:712	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	67	theme	Infrared	653:660	arg1	Reflectance					692:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance	635:702	Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR)	635:713	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	68	theme	Transition	912:921	arg1	diffraction					615:625	X-ray powder diffraction	602:625	X-ray powder diffraction (XRPD)	602:632	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	4	68	theme	Transition	912:921	arg1	spectroscopy					934:945	Transition UV-Visible spectroscopy	912:945	Transition UV-Visible spectroscopy	912:945	The products obtained were analyzed with X-ray powder diffraction (XRPD), Fourier-Transform Infrared Spectroscopy-Attenuated Total Reflectance (FTIR-ATR), Field Emission Scanning Electron Microscopy (FESEM), Diffuse Reflectance UV-Vis-NIR spectrometer, X-Ray pHotoelectron Spectroscopy (XPS), Thermogravimetric Analysis (TGA), N2 adsorption analysis, Transition UV-Visible spectroscopy, and Gas Chromatography-Mass Spectroscopy (GC-MS).					
31518870	5	69	theme	antibacterial	1008:1020	arg1	activity					1037:1044	antibacterial and anticancer activity	1008:1044	antibacterial and anticancer activity in melanoma skin cell	1008:1066	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	1	70	theme	zinc	129:132	arg1	BioMOF					173:178	BioMOF	173:178	BioMOF	173:178	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	1	70	theme	zinc	129:132	arg1	Framework					162:170	the zinc glutamate Bio Metal-Organic Framework	125:170	the zinc glutamate Bio Metal-Organic Framework (BioMOF)	125:179	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	1	71	theme	dual	217:220	arg1	material					222:229	a dual material	215:229	a dual material for nitric oxide (NO) and 5-fluorouracil (5FU)	215:276	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	2	72	theme	5FU	443:445	arg1	incorporation					419:431	the incorporation	415:431	the incorporation of NO and 5FU	415:445	In situ synthesis of BioMOF on the cotton fabric was successfully achieved and the incorporation of NO and 5FU was studied.					
31518870	3	73	theme	NO	543:544	arg1	incorporation					546:558	NO incorporation	543:558	NO incorporation	543:558	We have observed that BioMOF doped with FeII metal ions has a beneficial impact on NO incorporation.					
31518870	5	74	theme	treated	1155:1161	arg1	fabrics					1173:1179	treated cellulose fabrics	1155:1179	treated cellulose fabrics	1155:1179	Moreover, antibacterial and anticancer activity in melanoma skin cell was carried out in order to prove the biological activity of the drug-loaded BioMOF on treated cellulose fabrics.					
31518870	1	75	theme	glutamate	134:142	arg1	BioMOF					173:178	BioMOF	173:178	BioMOF	173:178	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
31518870	1	75	theme	glutamate	134:142	arg1	Framework					162:170	the zinc glutamate Bio Metal-Organic Framework	125:170	the zinc glutamate Bio Metal-Organic Framework (BioMOF)	125:179	This work describes in situ synthesis and application of the zinc glutamate Bio Metal-Organic Framework (BioMOF) supported on cellulose fabrics as a dual material for nitric oxide (NO) and 5-fluorouracil (5FU) controlled delivery for wound and/or skin cancer therapy.					
29751895	4	0	theme	fillets	962:968	arg1	higher					982:987	higher	982:987	higher	982:987	After storage of 15 days, the shear forces of coated fillets were 28-53% higher than that of control samples.					
29751895	4	0	theme	fillets	962:968	arg1	forces					945:950	the shear forces	935:950	the shear forces of coated fillets	935:968	After storage of 15 days, the shear forces of coated fillets were 28-53% higher than that of control samples.					
29751895	5	1	theme	glycerol	1089:1096	arg1	monolaurate					1098:1108	glycerol monolaurate	1089:1108	glycerol monolaurate	1089:1108	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	3	2	theme	TCA-soluble	866:876	arg1	peptides					878:885	TCA-soluble peptides	866:885	TCA-soluble peptides	866:885	Besides, the treatments also retarded the proteolytic degradation through quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern.					
29751895	3	3	theme	quantifiable	803:814	arg1	proteins					850:857	salt-soluble proteins	837:857	salt-soluble proteins (SSP)	837:863	Besides, the treatments also retarded the proteolytic degradation through quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern.					
29751895	3	3	theme	quantifiable	803:814	arg1	peptides					878:885	TCA-soluble peptides	866:885	TCA-soluble peptides	866:885	Besides, the treatments also retarded the proteolytic degradation through quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern.					
29751895	3	3	theme	quantifiable	803:814	arg1	pattern					900:906	SDS-PAGE pattern	891:906	SDS-PAGE pattern	891:906	Besides, the treatments also retarded the proteolytic degradation through quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern.					
29751895	3	3	theme	quantifiable	803:814	arg1	indicators					816:825	quantifiable indicators	803:825	quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern	803:906	Besides, the treatments also retarded the proteolytic degradation through quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern.					
29751895	0	4	theme	proteolytic	79:89	arg1	degradation					91:101	proteolytic degradation	79:101	proteolytic degradation	79:101	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	0	5	from	effects	11:17	arg1	activities					67:76	endogenous enzyme activities	49:76	endogenous enzyme activities	49:76	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	0	5	from	effects	11:17	arg1	degradation					91:101	proteolytic degradation	79:101	proteolytic degradation	79:101	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	0	5	from	effects	11:17	arg1	softening					115:123	texture softening	107:123	texture softening	107:123	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	1	6	theme	endogenous	263:272	arg1	activities					281:290	endogenous enzyme activities	263:290	endogenous enzyme activities	263:290	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	0	7	theme	texture	107:113	arg1	softening					115:123	texture softening	107:123	texture softening	107:123	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	5	8	theme	essential	1120:1128	arg1	oil					1130:1132	clove essential oil	1114:1132	clove essential oil	1114:1132	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	2	9	theme	fillets	599:605	arg1	B					564:564	cathepsin B	554:564	cathepsin B	554:564	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	9	theme	fillets	599:605	arg1	B + L					567:571	B + L	567:571	B + L	567:571	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	9	theme	fillets	599:605	arg1	activities					585:594	calpain activities	577:594	calpain activities	577:594	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	5	10	theme	refrigerated	1158:1169	arg1	fillets					1182:1188	refrigerated grass carp fillets	1158:1188	refrigerated grass carp fillets	1158:1188	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	1	11	theme	enzyme	274:279	arg1	activities					281:290	endogenous enzyme activities	263:290	endogenous enzyme activities	263:290	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	4	12	theme	15 days	926:932	arg1	storage					915:921	storage	915:921	storage of 15 days	915:932	After storage of 15 days, the shear forces of coated fillets were 28-53% higher than that of control samples.					
29751895	1	13	dep	fillets	380:386	arg1	stored					388:393	stored	388:393	fillets stored at 4 °C for 15 days	380:413	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	2	14	theme	%	638:638	arg1	reductions					622:631	the most reductions	613:631	the most reductions of 58%, 65% and 41%, respectively	613:665	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	3	15	theme	SDS-PAGE	891:898	arg1	pattern					900:906	SDS-PAGE pattern	891:906	SDS-PAGE pattern	891:906	Besides, the treatments also retarded the proteolytic degradation through quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern.					
29751895	5	16	theme	endogenous	1205:1214	arg1	enzyme					1216:1221	endogenous enzyme	1205:1221	endogenous enzyme induced proteolysis and softening	1205:1255	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	4	17	theme	shear	939:943	arg1	higher					982:987	higher	982:987	higher	982:987	After storage of 15 days, the shear forces of coated fillets were 28-53% higher than that of control samples.					
29751895	4	17	theme	shear	939:943	arg1	forces					945:950	the shear forces	935:950	the shear forces of coated fillets	935:968	After storage of 15 days, the shear forces of coated fillets were 28-53% higher than that of control samples.					
29751895	2	18	theme	fillets	720:726	arg1	values					702:707	the corresponding values	684:707	the corresponding values of control fillets	684:726	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	19	theme	coating	463:469	arg1	worse					476:480	worse	476:480	worse	476:480	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	19	theme	coating	463:469	arg1	effects					443:449	the inhibitory effects	428:449	the inhibitory effects of chitosan coating	428:469	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	5	20	theme	fillets	1182:1188	arg1	quality					1147:1153	the quality	1143:1153	the quality of refrigerated grass carp fillets	1143:1188	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	2	21	theme	%	651:651	arg1	reductions					622:631	the most reductions	613:631	the most reductions of 58%, 65% and 41%, respectively	613:665	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	0	22	theme	Inhibitory	0:9	arg1	effects					11:17	Inhibitory effects	0:17	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus)	0:164	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	2	23	theme	control	712:718	arg1	fillets					720:726	control fillets	712:726	control fillets	712:726	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	24	theme	chitosan	454:461	arg1	coating					463:469	chitosan coating	454:469	chitosan coating	454:469	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	25	from	worse	476:480	arg1	general					419:425	general	419:425	general	419:425	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	26	theme	most	617:620	arg1	reductions					622:631	the most reductions	613:631	the most reductions of 58%, 65% and 41%, respectively	613:665	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	27	theme	cathepsin	554:562	arg1	B					564:564	cathepsin B	554:564	cathepsin B	554:564	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	1	28	theme	Ctenopharyngodon	354:369	arg1	carp					348:351	grass carp	342:351	grass carp (Ctenopharyngodon idellus)	342:378	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	1	28	theme	Ctenopharyngodon	354:369	arg1	idellus					371:377	Ctenopharyngodon idellus	354:377	Ctenopharyngodon idellus	354:377	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	0	29	theme	chitosan-based	22:35	arg1	coatings					37:44	chitosan-based coatings	22:44	chitosan-based coatings	22:44	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	1	30	theme	proteolytic	293:303	arg1	degradation					305:315	proteolytic degradation	293:315	proteolytic degradation	293:315	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	0	31	theme	carp	134:137	arg1	activities					67:76	endogenous enzyme activities	49:76	endogenous enzyme activities	49:76	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	0	31	theme	carp	134:137	arg1	degradation					91:101	proteolytic degradation	79:101	proteolytic degradation	79:101	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	0	31	theme	carp	134:137	arg1	softening					115:123	texture softening	107:123	texture softening	107:123	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	2	32	theme	%	643:643	arg1	reductions					622:631	the most reductions	613:631	the most reductions of 58%, 65% and 41%, respectively	613:665	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	33	theme	inhibitory	432:441	arg1	worse					476:480	worse	476:480	worse	476:480	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	33	theme	inhibitory	432:441	arg1	effects					443:449	the inhibitory effects	428:449	the inhibitory effects of chitosan coating	428:469	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	5	34	theme	grass	1171:1175	arg1	carp					1177:1180	grass carp	1171:1180	refrigerated grass carp fillets	1158:1188	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	0	35	theme	grass	128:132	arg1	carp					134:137	grass carp	128:137	grass carp (Ctenopharyngodon idellus)	128:164	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	0	35	theme	grass	128:132	arg1	idellus					157:163	Ctenopharyngodon idellus	140:163	Ctenopharyngodon idellus	140:163	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	5	36	theme	carp	1177:1180	arg1	fillets					1182:1188	refrigerated grass carp fillets	1158:1188	refrigerated grass carp fillets	1158:1188	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	0	37	theme	Ctenopharyngodon	140:155	arg1	carp					134:137	grass carp	128:137	grass carp (Ctenopharyngodon idellus)	128:164	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	0	37	theme	Ctenopharyngodon	140:155	arg1	idellus					157:163	Ctenopharyngodon idellus	140:163	Ctenopharyngodon idellus	140:163	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	0	38	theme	coatings	37:44	arg1	effects					11:17	Inhibitory effects	0:17	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus)	0:164	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	1	39	theme	texture	321:327	arg1	softening					329:337	texture softening	321:337	texture softening	321:337	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	5	40	theme	chitosan	1048:1055	arg1	coating					1067:1073	chitosan composite coating	1048:1073	chitosan composite coating combined with glycerol monolaurate and clove essential oil	1048:1132	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	4	41	theme	control	1002:1008	arg1	samples					1010:1016	control samples	1002:1016	control samples	1002:1016	After storage of 15 days, the shear forces of coated fillets were 28-53% higher than that of control samples.					
29751895	1	42	theme	inhibitory	214:223	arg1	effects					225:231	the inhibitory effects	210:231	the inhibitory effects of chitosan-based coatings on endogenous enzyme activities	210:290	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	0	43	theme	enzyme	60:65	arg1	activities					67:76	endogenous enzyme activities	49:76	endogenous enzyme activities	49:76	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	5	44	theme	composite	1057:1065	arg1	coating					1067:1073	chitosan composite coating	1048:1073	chitosan composite coating combined with glycerol monolaurate and clove essential oil	1048:1132	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	5	45	theme	clove	1114:1118	arg1	oil					1130:1132	clove essential oil	1114:1132	clove essential oil	1114:1132	These results indicated that chitosan composite coating combined with glycerol monolaurate and clove essential oil improved the quality of refrigerated grass carp fillets by alleviating endogenous enzyme induced proteolysis and softening.					
29751895	0	46	theme	endogenous	49:58	arg1	activities					67:76	endogenous enzyme activities	49:76	endogenous enzyme activities	49:76	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	2	47	theme	calpain	577:583	arg1	activities					585:594	calpain activities	577:594	calpain activities	577:594	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	0	48	dep	fillets	166:172	arg1	stored					174:179	stored	174:179	fillets stored at 4 °C	166:187	Inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C.					
29751895	1	49	theme	grass	342:346	arg1	carp					348:351	grass carp	342:351	grass carp (Ctenopharyngodon idellus)	342:378	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	1	49	theme	grass	342:346	arg1	idellus					371:377	Ctenopharyngodon idellus	354:377	Ctenopharyngodon idellus	354:377	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	1	50	from	effects	225:231	arg1	activities					281:290	endogenous enzyme activities	263:290	endogenous enzyme activities	263:290	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	4	51	theme	coated	955:960	arg1	fillets					962:968	coated fillets	955:968	coated fillets	955:968	After storage of 15 days, the shear forces of coated fillets were 28-53% higher than that of control samples.					
29751895	2	52	from	general	419:425	arg1	worse					476:480	worse	476:480	worse	476:480	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	52	from	general	419:425	arg1	effects					443:449	the inhibitory effects	428:449	the inhibitory effects of chitosan coating	428:469	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	3	53	theme	proteolytic	771:781	arg1	degradation					783:793	the proteolytic degradation	767:793	the proteolytic degradation	767:793	Besides, the treatments also retarded the proteolytic degradation through quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern.					
29751895	2	54	theme	composite	504:512	arg1	coatings					514:521	chitosan composite coatings	495:521	chitosan composite coatings	495:521	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	2	55	theme	corresponding	688:700	arg1	values					702:707	the corresponding values	684:707	the corresponding values of control fillets	684:726	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	3	56	theme	salt-soluble	837:848	arg1	proteins					850:857	salt-soluble proteins	837:857	salt-soluble proteins (SSP)	837:863	Besides, the treatments also retarded the proteolytic degradation through quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern.					
29751895	3	56	theme	salt-soluble	837:848	arg1	SSP					860:862	SSP	860:862	SSP	860:862	Besides, the treatments also retarded the proteolytic degradation through quantifiable indicators including salt-soluble proteins (SSP), TCA-soluble peptides and SDS-PAGE pattern.					
29751895	1	57	theme	chitosan-based	236:249	arg1	coatings					251:258	chitosan-based coatings	236:258	chitosan-based coatings	236:258	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	2	58	theme	chitosan	495:502	arg1	coatings					514:521	chitosan composite coatings	495:521	chitosan composite coatings	495:521	In general, the inhibitory effects of chitosan coating were worse than that of chitosan composite coatings, which significantly inhibited cathepsin B, B + L and calpain activities of fillets, with the most reductions of 58%, 65% and 41%, respectively when compared to the corresponding values of control fillets.					
29751895	1	59	theme	carp	348:351	arg1	degradation					305:315	proteolytic degradation	293:315	proteolytic degradation	293:315	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	1	59	theme	carp	348:351	arg1	softening					329:337	texture softening	321:337	texture softening	321:337	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29751895	1	60	theme	coatings	251:258	arg1	effects					225:231	the inhibitory effects	210:231	the inhibitory effects of chitosan-based coatings on endogenous enzyme activities	210:290	The study evaluated the inhibitory effects of chitosan-based coatings on endogenous enzyme activities, proteolytic degradation and texture softening of grass carp (Ctenopharyngodon idellus) fillets stored at 4 °C for 15 days.					
29578067	11	0	theme	antimicrobial-induced	1962:1982	arg1	changes					1984:1990	these important antimicrobial-induced changes	1946:1990	these important antimicrobial-induced changes	1946:1990	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	2	1	dep	composition	453:463	arg1	the					449:451	the	449:451	the	449:451	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	5	2	theme	Miseq-based	1106:1116	arg1	sequencing					1118:1127	Illumina Miseq-based sequencing	1097:1127	Illumina Miseq-based sequencing	1097:1127	Genomic DNA was extracted, and the V1-V3 hypervariable region of 16S rRNA gene was amplified and sequenced using Illumina Miseq-based sequencing.					
29578067	7	3	theme	fecal	1352:1356	arg1	microbiota					1358:1367	fecal microbiota	1352:1367	fecal microbiota	1352:1367	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	1	4	theme	mucosal	270:276	arg1	communities					288:298	potentially beneficent mucosal microbial communities	247:298	potentially beneficent mucosal microbial communities	247:298	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	9	5	theme	day	1757:1759	arg1	composition					1763:1773	the day 0 composition	1753:1773	the day 0 composition	1753:1773	In contrast, animals that received PPG, OTC and CCFA, showed a tendency towards a balanced homeostatic microbiota structure on day 7, but appeared to deviate away from the day 0 composition by day 14.					
29578067	3	6	theme	Ceftiofur	700:708	arg1	antimicrobials					644:657	the antimicrobials	640:657	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL)	640:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	6	theme	Ceftiofur	700:708	arg1	hydrochloride					710:722	Ceftiofur hydrochloride	700:722	Ceftiofur hydrochloride (CHC)	700:728	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	6	theme	Ceftiofur	700:708	arg1	CHC					725:727	CHC	725:727	CHC	725:727	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	6	7	theme	abundant	1158:1165	arg1	Firmicutes					1178:1187	Firmicutes	1178:1187	Firmicutes	1178:1187	Across all groups, the most abundant phyla were Firmicutes, Bacteroidetes, and Proteobacteria.					
29578067	6	7	theme	abundant	1158:1165	arg1	phyla					1167:1171	the most abundant phyla	1149:1171	the most abundant phyla	1149:1171	Across all groups, the most abundant phyla were Firmicutes, Bacteroidetes, and Proteobacteria.					
29578067	3	8	theme	treatment	548:556	arg1	groups					558:563	Five antimicrobial treatment groups	529:563	Five antimicrobial treatment groups	529:563	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	8	theme	treatment	548:556	arg1	each					566:569	each	566:569	each	566:569	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	0	9	theme	pigs	116:119	arg1	microbiota					94:103	the fecal microbiota	84:103	the fecal microbiota of growing pigs	84:119	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	1	10	theme	disease	211:217	arg1	treatment					187:195	treatment	187:195	treatment	187:195	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	1	10	theme	disease	211:217	arg1	prevention					172:181	prevention	172:181	prevention	172:181	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	4	11	theme	antimicrobial	888:900	arg1	control = day					918:930	control = day 0	918:932	control = day 0	918:932	Individual fecal swabs were collected immediately before antimicrobial administration (control = day 0), and again on days 1, 3, 7, and 14 after dosing.					
29578067	4	11	theme	antimicrobial	888:900	arg1	administration					902:915	antimicrobial administration	888:915	antimicrobial administration (control = day 0)	888:933	Individual fecal swabs were collected immediately before antimicrobial administration (control = day 0), and again on days 1, 3, 7, and 14 after dosing.					
29578067	7	12	theme	pronounced	1288:1297	arg1	shift					1324:1328	a pronounced, antimicrobial-dependent shift	1286:1328	a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota	1286:1367	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	4	13	theme	fecal	842:846	arg1	swabs					848:852	Individual fecal swabs	831:852	Individual fecal swabs	831:852	Individual fecal swabs were collected immediately before antimicrobial administration (control = day 0), and again on days 1, 3, 7, and 14 after dosing.					
29578067	3	14	theme	eight-week	591:600	arg1	piglets					606:612	eight-week old piglets	591:612	eight-week old piglets	591:612	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	14	theme	eight-week	591:600	arg1	four					585:588	four	585:588	four	585:588	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	7	15	theme	stacked	1258:1264	arg1	graphs					1271:1276	stacked area graphs	1258:1276	stacked area graphs	1258:1276	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	10	16	theme	fecal	1832:1836	arg1	microbiota					1838:1847	fecal microbiota	1832:1847	fecal microbiota	1832:1847	Based on our results, the observed changes in fecal microbiota showed antimicrobial-specific variation in both duration and extent.					
29578067	5	17	theme	V1-V3	1019:1023	arg1	region					1039:1044	the V1-V3 hypervariable region	1015:1044	the V1-V3 hypervariable region of 16S rRNA gene	1015:1061	Genomic DNA was extracted, and the V1-V3 hypervariable region of 16S rRNA gene was amplified and sequenced using Illumina Miseq-based sequencing.					
29578067	2	18	theme	administration	431:444	arg1	impact					398:403	the impact	394:403	the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs	394:526	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	1	19	theme	cost-effective	147:160	arg1	antimicrobials					128:141	antimicrobials	128:141	antimicrobials	128:141	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	1	19	theme	cost-effective	147:160	arg1	tools					162:166	cost-effective tools	147:166	cost-effective tools for prevention and treatment of infectious disease	147:217	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	2	20	theme	fecal	495:499	arg1	microbiota					501:510	the resident fecal microbiota	482:510	the resident fecal microbiota	482:510	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	0	21	from	Impact	0:5	arg1	diversity					71:79	diversity	71:79	diversity	71:79	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	0	21	from	Impact	0:5	arg1	structure					57:65	structure	57:65	structure	57:65	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	0	22	theme	fecal	88:92	arg1	microbiota					94:103	the fecal microbiota	84:103	the fecal microbiota of growing pigs	84:119	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	8	23	theme	microbial	1412:1420	arg1	compositions					1422:1433	the fecal microbial compositions	1402:1433	the fecal microbial compositions of the groups receiving CHC and TUL	1402:1469	By day 14, the fecal microbial compositions of the groups receiving CHC and TUL had returned to a distribution that closely resembled that observed on day 0, but differences were still evident.					
29578067	11	24	theme	antimicrobials	2041:2054	arg1	use					2034:2036	the use	2030:2036	the use of antimicrobials in health management programs in the swine industry	2030:2106	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	3	25	dep	dose	815:818	arg1	label					809:813	label	809:813	label	809:813	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	1	26	theme	microbial	278:286	arg1	communities					288:298	potentially beneficent mucosal microbial communities	247:298	potentially beneficent mucosal microbial communities	247:298	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	7	27	theme	discriminant	1232:1243	arg1	analysis					1245:1252	Linear discriminant analysis	1225:1252	Linear discriminant analysis	1225:1252	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	9	28	theme	balanced	1667:1674	arg1	structure					1699:1707	a balanced homeostatic microbiota structure	1665:1707	a balanced homeostatic microbiota structure	1665:1707	In contrast, animals that received PPG, OTC and CCFA, showed a tendency towards a balanced homeostatic microbiota structure on day 7, but appeared to deviate away from the day 0 composition by day 14.					
29578067	8	29	theme	groups	1442:1447	arg1	compositions					1422:1433	the fecal microbial compositions	1402:1433	the fecal microbial compositions of the groups receiving CHC and TUL	1402:1469	By day 14, the fecal microbial compositions of the groups receiving CHC and TUL had returned to a distribution that closely resembled that observed on day 0, but differences were still evident.					
29578067	2	30	theme	antibiotics	419:429	arg1	administration					431:444	parenteral antibiotics administration	408:444	parenteral antibiotics administration	408:444	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	0	31	theme	parenteral	10:19	arg1	administration					35:48	parenteral antimicrobial administration	10:48	parenteral antimicrobial administration	10:48	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	9	32	theme	microbiota	1688:1697	arg1	structure					1699:1707	a balanced homeostatic microbiota structure	1665:1707	a balanced homeostatic microbiota structure	1665:1707	In contrast, animals that received PPG, OTC and CCFA, showed a tendency towards a balanced homeostatic microbiota structure on day 7, but appeared to deviate away from the day 0 composition by day 14.					
29578067	0	33	theme	administration	35:48	arg1	Impact					0:5	Impact	0:5	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.	0:120	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	1	34	theme	pigs	311:314	arg1	impact					224:229	the impact	220:229	the impact of their use on potentially beneficent mucosal microbial communities of growing pigs	220:314	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	3	35	theme	Penicillin	763:772	arg1	antimicrobials					644:657	the antimicrobials	640:657	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL)	640:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	35	theme	Penicillin	763:772	arg1	PPG					777:779	PPG	777:779	PPG	777:779	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	35	theme	Penicillin	763:772	arg1	G					774:774	Procaine Penicillin G	754:774	Procaine Penicillin G (PPG)	754:780	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	5	36	theme	16S	1049:1051	arg1	rRNA					1053:1056	16S rRNA	1049:1056	16S rRNA gene	1049:1061	Genomic DNA was extracted, and the V1-V3 hypervariable region of 16S rRNA gene was amplified and sequenced using Illumina Miseq-based sequencing.					
29578067	5	37	theme	gene	1058:1061	arg1	region					1039:1044	the V1-V3 hypervariable region	1015:1044	the V1-V3 hypervariable region of 16S rRNA gene	1015:1061	Genomic DNA was extracted, and the V1-V3 hypervariable region of 16S rRNA gene was amplified and sequenced using Illumina Miseq-based sequencing.					
29578067	3	38	theme	Crystalline	670:680	arg1	antimicrobials					644:657	the antimicrobials	640:657	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL)	640:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	38	theme	Crystalline	670:680	arg1	CCFA					693:696	CCFA	693:696	CCFA	693:696	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	38	theme	Crystalline	670:680	arg1	acid					687:690	Ceftiofur Crystalline free acid	660:690	Ceftiofur Crystalline free acid (CCFA)	660:697	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	2	39	theme	study	368:372	arg1	objective					350:358	The objective	346:358	The objective of this study	346:372	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	3	40	dep	antimicrobials	644:657	arg1	hydrochloride					710:722	Ceftiofur hydrochloride	700:722	Ceftiofur hydrochloride (CHC)	700:728	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	TUL					801:803	TUL	801:803	TUL	801:803	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	PPG					777:779	PPG	777:779	PPG	777:779	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	OTC					748:750	OTC	748:750	OTC	748:750	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	CHC					725:727	CHC	725:727	CHC	725:727	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	antimicrobials					644:657	the antimicrobials	640:657	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL)	640:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	CCFA					693:696	CCFA	693:696	CCFA	693:696	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	Tulathromycin					786:798	Tulathromycin	786:798	Tulathromycin (TUL)	786:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	Oxytetracycline					731:745	Oxytetracycline	731:745	Oxytetracycline (OTC)	731:751	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	G					774:774	Procaine Penicillin G	754:774	Procaine Penicillin G (PPG)	754:780	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	40	dep	antimicrobials	644:657	arg1	acid					687:690	Ceftiofur Crystalline free acid	660:690	Ceftiofur Crystalline free acid (CCFA)	660:697	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	11	41	theme	health	2059:2064	arg1	programs					2077:2084	health management programs	2059:2084	health management programs in the swine industry	2059:2106	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	5	42	theme	Illumina	1097:1104	arg1	sequencing					1118:1127	Illumina Miseq-based sequencing	1097:1127	Illumina Miseq-based sequencing	1097:1127	Genomic DNA was extracted, and the V1-V3 hypervariable region of 16S rRNA gene was amplified and sequenced using Illumina Miseq-based sequencing.					
29578067	11	43	theme	changes	1984:1990	arg1	impact					1936:1941	the impact	1932:1941	the impact of these important antimicrobial-induced changes	1932:1990	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	1	44	theme	beneficent	259:268	arg1	communities					288:298	potentially beneficent mucosal microbial communities	247:298	potentially beneficent mucosal microbial communities	247:298	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	11	45	theme	important	1952:1960	arg1	changes					1984:1990	these important antimicrobial-induced changes	1946:1990	these important antimicrobial-induced changes	1946:1990	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	7	46	theme	microbiota	1358:1367	arg1	composition					1337:1347	the composition	1333:1347	the composition of fecal microbiota	1333:1367	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	3	47	theme	antimicrobial	534:546	arg1	groups					558:563	Five antimicrobial treatment groups	529:563	Five antimicrobial treatment groups	529:563	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	47	theme	antimicrobial	534:546	arg1	each					566:569	each	566:569	each	566:569	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	11	48	theme	critical	2002:2009	arg1	step					2011:2014	a critical step	2000:2014	a critical step	2000:2014	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	11	49	theme	management	2066:2075	arg1	programs					2077:2084	health management programs	2059:2084	health management programs in the swine industry	2059:2106	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	7	50	dep	pronounced	1288:1297	arg1	antimicrobial-dependent					1300:1322	antimicrobial-dependent	1300:1322	antimicrobial-dependent	1300:1322	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	8	51	theme	fecal	1406:1410	arg1	compositions					1422:1433	the fecal microbial compositions	1402:1433	the fecal microbial compositions of the groups receiving CHC and TUL	1402:1469	By day 14, the fecal microbial compositions of the groups receiving CHC and TUL had returned to a distribution that closely resembled that observed on day 0, but differences were still evident.					
29578067	1	52	theme	infectious	200:209	arg1	disease					211:217	infectious disease	200:217	infectious disease	200:217	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	7	53	from	day	1384:1386	arg1	time					1374:1377	time	1374:1377	time from day 0	1374:1388	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	5	54	theme	Genomic	984:990	arg1	DNA					992:994	Genomic DNA	984:994	Genomic DNA	984:994	Genomic DNA was extracted, and the V1-V3 hypervariable region of 16S rRNA gene was amplified and sequenced using Illumina Miseq-based sequencing.					
29578067	3	55	theme	old	602:604	arg1	piglets					606:612	eight-week old piglets	591:612	eight-week old piglets	591:612	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	55	theme	old	602:604	arg1	four					585:588	four	585:588	four	585:588	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	56	theme	Procaine	754:761	arg1	antimicrobials					644:657	the antimicrobials	640:657	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL)	640:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	56	theme	Procaine	754:761	arg1	PPG					777:779	PPG	777:779	PPG	777:779	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	56	theme	Procaine	754:761	arg1	G					774:774	Procaine Penicillin G	754:774	Procaine Penicillin G (PPG)	754:780	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	4	57	theme	Individual	831:840	arg1	swabs					848:852	Individual fecal swabs	831:852	Individual fecal swabs	831:852	Individual fecal swabs were collected immediately before antimicrobial administration (control = day 0), and again on days 1, 3, 7, and 14 after dosing.					
29578067	1	58	from	impact	224:229	arg1	communities					288:298	potentially beneficent mucosal microbial communities	247:298	potentially beneficent mucosal microbial communities	247:298	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	7	59	from	shift	1324:1328	arg1	composition					1337:1347	the composition	1333:1347	the composition of fecal microbiota	1333:1367	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	2	60	theme	microbiota	501:510	arg1	diversity					469:477	diversity	469:477	diversity	469:477	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	2	60	theme	microbiota	501:510	arg1	composition					453:463	composition	453:463	composition	453:463	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	7	61	theme	area	1266:1269	arg1	graphs					1271:1276	stacked area graphs	1258:1276	stacked area graphs	1258:1276	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	5	62	theme	hypervariable	1025:1037	arg1	region					1039:1044	the V1-V3 hypervariable region	1015:1044	the V1-V3 hypervariable region of 16S rRNA gene	1015:1061	Genomic DNA was extracted, and the V1-V3 hypervariable region of 16S rRNA gene was amplified and sequenced using Illumina Miseq-based sequencing.					
29578067	2	63	theme	resident	486:493	arg1	microbiota					501:510	the resident fecal microbiota	482:510	the resident fecal microbiota	482:510	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	2	64	from	composition	453:463	arg1	pigs					523:526	growing pigs	515:526	growing pigs	515:526	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	11	65	from	programs	2077:2084	arg1	industry					2099:2106	the swine industry	2089:2106	the swine industry	2089:2106	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	0	66	theme	microbiota	94:103	arg1	diversity					71:79	diversity	71:79	diversity	71:79	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	0	66	theme	microbiota	94:103	arg1	structure					57:65	structure	57:65	structure	57:65	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	2	67	from	diversity	469:477	arg1	pigs					523:526	growing pigs	515:526	growing pigs	515:526	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	10	68	theme	antimicrobial-specific	1856:1877	arg1	variation					1879:1887	antimicrobial-specific variation	1856:1887	antimicrobial-specific variation in both duration and extent	1856:1915	Based on our results, the observed changes in fecal microbiota showed antimicrobial-specific variation in both duration and extent.					
29578067	0	69	theme	growing	108:114	arg1	pigs					116:119	growing pigs	108:119	growing pigs	108:119	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	2	70	theme	growing	515:521	arg1	pigs					523:526	growing pigs	515:526	growing pigs	515:526	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	10	71	from	changes	1821:1827	arg1	microbiota					1838:1847	fecal microbiota	1832:1847	fecal microbiota	1832:1847	Based on our results, the observed changes in fecal microbiota showed antimicrobial-specific variation in both duration and extent.					
29578067	7	72	theme	Linear	1225:1230	arg1	analysis					1245:1252	Linear discriminant analysis	1225:1252	Linear discriminant analysis	1225:1252	Linear discriminant analysis and stacked area graphs, showed a pronounced, antimicrobial-dependent shift in the composition of fecal microbiota over time from day 0.					
29578067	0	73	theme	antimicrobial	21:33	arg1	administration					35:48	parenteral antimicrobial administration	10:48	parenteral antimicrobial administration	10:48	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	9	74	theme	homeostatic	1676:1686	arg1	structure					1699:1707	a balanced homeostatic microbiota structure	1665:1707	a balanced homeostatic microbiota structure	1665:1707	In contrast, animals that received PPG, OTC and CCFA, showed a tendency towards a balanced homeostatic microbiota structure on day 7, but appeared to deviate away from the day 0 composition by day 14.					
29578067	3	75	theme	antimicrobials	644:657	arg1	hydrochloride					710:722	Ceftiofur hydrochloride	700:722	Ceftiofur hydrochloride (CHC)	700:728	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	75	theme	antimicrobials	644:657	arg1	antimicrobials					644:657	the antimicrobials	640:657	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL)	640:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	75	theme	antimicrobials	644:657	arg1	Tulathromycin					786:798	Tulathromycin	786:798	Tulathromycin (TUL)	786:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	75	theme	antimicrobials	644:657	arg1	one					633:635	one	633:635	one	633:635	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	75	theme	antimicrobials	644:657	arg1	Oxytetracycline					731:745	Oxytetracycline	731:745	Oxytetracycline (OTC)	731:751	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	75	theme	antimicrobials	644:657	arg1	G					774:774	Procaine Penicillin G	754:774	Procaine Penicillin G (PPG)	754:780	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	75	theme	antimicrobials	644:657	arg1	acid					687:690	Ceftiofur Crystalline free acid	660:690	Ceftiofur Crystalline free acid (CCFA)	660:697	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	2	76	theme	parenteral	408:417	arg1	administration					431:444	parenteral antibiotics administration	408:444	parenteral antibiotics administration	408:444	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	1	77	theme	growing	303:309	arg1	pigs					311:314	growing pigs	303:314	growing pigs	303:314	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	0	78	dep	structure	57:65	arg1	the					53:55	the	53:55	the	53:55	Impact of parenteral antimicrobial administration on the structure and diversity of the fecal microbiota of growing pigs.					
29578067	11	79	theme	swine	2093:2097	arg1	industry					2099:2106	the swine industry	2089:2106	the swine industry	2089:2106	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	5	80	theme	rRNA	1053:1056	arg1	gene					1058:1061	16S rRNA gene	1049:1061	16S rRNA gene	1049:1061	Genomic DNA was extracted, and the V1-V3 hypervariable region of 16S rRNA gene was amplified and sequenced using Illumina Miseq-based sequencing.					
29578067	10	81	from	variation	1879:1887	arg1	extent					1910:1915	extent	1910:1915	extent	1910:1915	Based on our results, the observed changes in fecal microbiota showed antimicrobial-specific variation in both duration and extent.					
29578067	10	81	from	variation	1879:1887	arg1	duration					1897:1904	duration	1897:1904	duration	1897:1904	Based on our results, the observed changes in fecal microbiota showed antimicrobial-specific variation in both duration and extent.					
29578067	3	82	theme	free	682:685	arg1	antimicrobials					644:657	the antimicrobials	640:657	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL)	640:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	82	theme	free	682:685	arg1	CCFA					693:696	CCFA	693:696	CCFA	693:696	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	82	theme	free	682:685	arg1	acid					687:690	Ceftiofur Crystalline free acid	660:690	Ceftiofur Crystalline free acid (CCFA)	660:697	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	83	theme	Ceftiofur	660:668	arg1	antimicrobials					644:657	the antimicrobials	640:657	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL)	640:804	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	83	theme	Ceftiofur	660:668	arg1	CCFA					693:696	CCFA	693:696	CCFA	693:696	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	3	83	theme	Ceftiofur	660:668	arg1	acid					687:690	Ceftiofur Crystalline free acid	660:690	Ceftiofur Crystalline free acid (CCFA)	660:697	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Oxytetracycline (OTC), Procaine Penicillin G (PPG) and Tulathromycin (TUL) at label dose and route.					
29578067	2	84	from	impact	398:403	arg1	diversity					469:477	diversity	469:477	diversity	469:477	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	2	84	from	impact	398:403	arg1	composition					453:463	composition	453:463	composition	453:463	The objective of this study was to characterize the impact of parenteral antibiotics administration on the composition and diversity of the resident fecal microbiota in growing pigs.					
29578067	11	85	from	use	2034:2036	arg1	programs					2077:2084	health management programs	2059:2084	health management programs in the swine industry	2059:2106	Understanding the impact of these important antimicrobial-induced changes will be a critical step in optimizing the use of antimicrobials in health management programs in the swine industry.					
29578067	1	86	theme	use	240:242	arg1	impact					224:229	the impact	220:229	the impact of their use on potentially beneficent mucosal microbial communities of growing pigs	220:314	While antimicrobials are cost-effective tools for prevention and treatment of infectious disease, the impact of their use on potentially beneficent mucosal microbial communities of growing pigs has not been widely explored.					
29578067	10	87	theme	observed	1812:1819	arg1	changes					1821:1827	the observed changes	1808:1827	the observed changes in fecal microbiota	1808:1847	Based on our results, the observed changes in fecal microbiota showed antimicrobial-specific variation in both duration and extent.					
31858315	0	0	theme	Ethyl	89:93	arg1	Cellulose					95:103	Ethyl Cellulose	89:103	Ethyl Cellulose	89:103	Sustained Release Ziprasidone Microparticles Prepared by Spray Drying with Soluplus® and Ethyl Cellulose to Eliminate Food Effect and Enhance Bioavailability.					
31858315	6	1	theme	Pharmacokinetic	967:981	arg1	studies					983:989	Pharmacokinetic studies	967:989	Pharmacokinetic studies in beagle dogs	967:1004	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	3	2	theme	amorphous	493:501	arg1	state					503:507	amorphous state	493:507	amorphous state in the spray-dried microparticles	493:541	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	6	3	theme	oral	1060:1063	arg1	bioavailability					1065:1079	oral bioavailability	1060:1079	oral bioavailability of the microparticles	1060:1101	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	4	4	theme	encapsulation	710:722	arg1	materials					724:732	Commonly used encapsulation materials	696:732	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose	696:791	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	4	4	theme	encapsulation	710:722	arg1	S100					768:771	Eudragit® RL, Eudragit® S100 and Ethyl Cellulose	744:791	S100	768:771	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	4	4	theme	encapsulation	710:722	arg1	Cellulose					783:791	Eudragit® RL, Eudragit® S100 and Ethyl Cellulose	744:791	Cellulose	783:791	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	6	5	theme	Tmax	1148:1151	arg1	value					1153:1157	a prolonged Tmax value	1136:1157	a prolonged Tmax value	1136:1157	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	3	6	from	state	503:507	arg1	microparticles					528:541	the spray-dried microparticles	512:541	the spray-dried microparticles	512:541	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	3	6	from	state	503:507	arg1	microparticles					608:621	the Soluplus®-ZIP composite microparticles	580:621	the Soluplus®-ZIP composite microparticles	580:621	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	6	7	theme	ZIP	1216:1218	arg1	capsules					1220:1227	the commercial ZIP capsules	1201:1227	the commercial ZIP capsules	1201:1227	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	6	8	theme	beagle	994:999	arg1	dogs					1001:1004	beagle dogs	994:1004	beagle dogs	994:1004	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	5	9	theme	sustained	938:946	arg1	behaviors					956:964	the most sustained release behaviors	929:964	the most sustained release behaviors	929:964	The formulation containing ethyl cellulose provided the most sustained release behaviors.					
31858315	3	10	from	microparticles	608:621	arg1	state					503:507	amorphous state	493:507	amorphous state in the spray-dried microparticles	493:541	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	3	10	from	microparticles	608:621	arg1	ZIP					571:573	ZIP	571:573	ZIP from the Soluplus®-ZIP composite microparticles	571:621	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	3	10	from	microparticles	608:621	arg1	rate					563:566	the dissolution rate	547:566	the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles	547:621	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	6	11	theme	significant	1034:1044	arg1	difference					1046:1055	no significant difference	1031:1055	no significant difference in oral bioavailability of the microparticles	1031:1101	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	0	12	theme	Food	118:121	arg1	Effect					123:128	Food Effect	118:128	Food Effect	118:128	Sustained Release Ziprasidone Microparticles Prepared by Spray Drying with Soluplus® and Ethyl Cellulose to Eliminate Food Effect and Enhance Bioavailability.					
31858315	6	13	from	difference	1046:1055	arg1	bioavailability					1065:1079	oral bioavailability	1060:1079	oral bioavailability of the microparticles	1060:1101	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	5	14	contain	containing	893:902	arg1	formulation					881:891	The formulation	877:891	The formulation containing ethyl cellulose	877:918	The formulation containing ethyl cellulose provided the most sustained release behaviors.					
31858315	5	14	contain	containing	893:902	arg2	cellulose					910:918	ethyl cellulose	904:918	ethyl cellulose	904:918	The formulation containing ethyl cellulose provided the most sustained release behaviors.					
31858315	3	15	theme	spray-dried	516:526	arg1	microparticles					528:541	the spray-dried microparticles	512:541	the spray-dried microparticles	512:541	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	6	16	theme	commercial	1205:1214	arg1	capsules					1220:1227	the commercial ZIP capsules	1201:1227	the commercial ZIP capsules	1201:1227	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	2	17	theme	solubility	418:427	arg1	Soluplus®					376:384	Soluplus®	376:384	Soluplus®	376:384	ZIP solid dispersions were produced via spray drying using Soluplus®, an amphiphilic polymer, as the solubility enhancer.					
31858315	2	17	theme	solubility	418:427	arg1	enhancer					429:436	the solubility enhancer	414:436	the solubility enhancer	414:436	ZIP solid dispersions were produced via spray drying using Soluplus®, an amphiphilic polymer, as the solubility enhancer.					
31858315	6	18	theme	prolonged	1138:1146	arg1	value					1153:1157	a prolonged Tmax value	1136:1157	a prolonged Tmax value	1136:1157	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	1	19	theme	study	179:183	arg1	purpose					163:169	The purpose	159:169	The purpose of this study	159:183	The purpose of this study was to develop and evaluate a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release.					
31858315	0	20	theme	Release	10:16	arg1	Microparticles					30:43	Sustained Release Ziprasidone Microparticles	0:43	Sustained Release Ziprasidone Microparticles Prepared by Spray Drying with Soluplus® and Ethyl Cellulose	0:103	Sustained Release Ziprasidone Microparticles Prepared by Spray Drying with Soluplus® and Ethyl Cellulose to Eliminate Food Effect and Enhance Bioavailability.					
31858315	4	21	theme	Ethyl	777:781	arg1	Cellulose					783:791	Eudragit® RL, Eudragit® S100 and Ethyl Cellulose	744:791	Cellulose	783:791	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	0	22	theme	Sustained	0:8	arg1	Microparticles					30:43	Sustained Release Ziprasidone Microparticles	0:43	Sustained Release Ziprasidone Microparticles Prepared by Spray Drying with Soluplus® and Ethyl Cellulose	0:103	Sustained Release Ziprasidone Microparticles Prepared by Spray Drying with Soluplus® and Ethyl Cellulose to Eliminate Food Effect and Enhance Bioavailability.					
31858315	1	23	theme	improved	256:263	arg1	absorption					278:287	improved fasted state absorption	256:287	improved fasted state absorption	256:287	The purpose of this study was to develop and evaluate a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release.					
31858315	3	24	theme	ZIP	571:573	arg1	state					503:507	amorphous state	493:507	amorphous state in the spray-dried microparticles	493:541	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	3	24	theme	ZIP	571:573	arg1	rate					563:566	the dissolution rate	547:566	the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles	547:621	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	1	25	theme	fasted	265:270	arg1	absorption					278:287	improved fasted state absorption	256:287	improved fasted state absorption	256:287	The purpose of this study was to develop and evaluate a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release.					
31858315	3	26	theme	dissolution	551:561	arg1	rate					563:566	the dissolution rate	547:566	the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles	547:621	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	6	27	theme	fed	1120:1122	arg1	states					1124:1129	fasted and fed states	1109:1129	fasted and fed states	1109:1129	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	0	28	theme	Ziprasidone	18:28	arg1	Microparticles					30:43	Sustained Release Ziprasidone Microparticles	0:43	Sustained Release Ziprasidone Microparticles Prepared by Spray Drying with Soluplus® and Ethyl Cellulose	0:103	Sustained Release Ziprasidone Microparticles Prepared by Spray Drying with Soluplus® and Ethyl Cellulose to Eliminate Food Effect and Enhance Bioavailability.					
31858315	1	29	theme	state	272:276	arg1	absorption					278:287	improved fasted state absorption	256:287	improved fasted state absorption	256:287	The purpose of this study was to develop and evaluate a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release.					
31858315	5	30	theme	release	948:954	arg1	behaviors					956:964	the most sustained release behaviors	929:964	the most sustained release behaviors	929:964	The formulation containing ethyl cellulose provided the most sustained release behaviors.					
31858315	4	31	theme	used	705:708	arg1	materials					724:732	Commonly used encapsulation materials	696:732	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose	696:791	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	4	31	theme	used	705:708	arg1	S100					768:771	Eudragit® RL, Eudragit® S100 and Ethyl Cellulose	744:791	S100	768:771	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	4	31	theme	used	705:708	arg1	Cellulose					783:791	Eudragit® RL, Eudragit® S100 and Ethyl Cellulose	744:791	Cellulose	783:791	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	6	32	theme	fasted	1109:1114	arg1	states					1124:1129	fasted and fed states	1109:1129	fasted and fed states	1109:1129	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	3	33	theme	Soluplus®-ZIP	584:596	arg1	microparticles					608:621	the Soluplus®-ZIP composite microparticles	580:621	the Soluplus®-ZIP composite microparticles	580:621	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	2	34	theme	amphiphilic	390:400	arg1	Soluplus®					376:384	Soluplus®	376:384	Soluplus®	376:384	ZIP solid dispersions were produced via spray drying using Soluplus®, an amphiphilic polymer, as the solubility enhancer.					
31858315	2	34	theme	amphiphilic	390:400	arg1	polymer					402:408	an amphiphilic polymer	387:408	an amphiphilic polymer	387:408	ZIP solid dispersions were produced via spray drying using Soluplus®, an amphiphilic polymer, as the solubility enhancer.					
31858315	6	35	theme	microparticles	1088:1101	arg1	bioavailability					1065:1079	oral bioavailability	1060:1079	oral bioavailability of the microparticles	1060:1101	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	3	36	theme	composite	598:606	arg1	microparticles					608:621	the Soluplus®-ZIP composite microparticles	580:621	the Soluplus®-ZIP composite microparticles	580:621	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	3	37	theme	Physicochemical	439:453	arg1	analysis					455:462	Physicochemical analysis	439:462	Physicochemical analysis	439:462	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	1	38	theme	sustained	293:301	arg1	release					308:314	sustained drug release	293:314	sustained drug release	293:314	The purpose of this study was to develop and evaluate a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release.					
31858315	4	39	theme	release	859:865	arg1	kinetics					867:874	the drug release kinetics	850:874	the drug release kinetics	850:874	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	4	40	theme	Eudragit®	744:752	arg1	Eudragit®					758:766	Eudragit®	758:766	Eudragit®	758:766	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	4	40	theme	Eudragit®	744:752	arg1	S100					768:771	Eudragit® RL, Eudragit® S100 and Ethyl Cellulose	744:791	S100	768:771	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	0	41	theme	Spray	57:61	arg1	Drying					63:68	Spray Drying	57:68	Spray Drying	57:68	Sustained Release Ziprasidone Microparticles Prepared by Spray Drying with Soluplus® and Ethyl Cellulose to Eliminate Food Effect and Enhance Bioavailability.					
31858315	1	42	theme	drug	303:306	arg1	release					308:314	sustained drug release	293:314	sustained drug release	293:314	The purpose of this study was to develop and evaluate a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release.					
31858315	4	43	theme	drug	854:857	arg1	kinetics					867:874	the drug release kinetics	850:874	the drug release kinetics	850:874	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	2	44	theme	solid	321:325	arg1	dispersions					327:337	ZIP solid dispersions	317:337	ZIP solid dispersions	317:337	ZIP solid dispersions were produced via spray drying using Soluplus®, an amphiphilic polymer, as the solubility enhancer.					
31858315	4	45	theme	solid	820:824	arg1	dispersions					826:836	the solid dispersions	816:836	the solid dispersions	816:836	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	2	46	theme	ZIP	317:319	arg1	dispersions					327:337	ZIP solid dispersions	317:337	ZIP solid dispersions	317:337	ZIP solid dispersions were produced via spray drying using Soluplus®, an amphiphilic polymer, as the solubility enhancer.					
31858315	3	47	from	rate	563:566	arg1	microparticles					528:541	the spray-dried microparticles	512:541	the spray-dried microparticles	512:541	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	3	47	from	rate	563:566	arg1	microparticles					608:621	the Soluplus®-ZIP composite microparticles	580:621	the Soluplus®-ZIP composite microparticles	580:621	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	1	48	theme	new	215:217	arg1	formulation					219:229	a new formulation	213:229	a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release	213:314	The purpose of this study was to develop and evaluate a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release.					
31858315	4	49	theme	RL	754:755	arg1	Eudragit®					758:766	Eudragit®	758:766	Eudragit®	758:766	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	4	49	theme	RL	754:755	arg1	S100					768:771	Eudragit® RL, Eudragit® S100 and Ethyl Cellulose	744:791	S100	768:771	Commonly used encapsulation materials including Eudragit® RL, Eudragit® S100 and Ethyl Cellulose were incorporated into the solid dispersions to regulate the drug release kinetics.					
31858315	3	50	dep	increased	641:649	arg1	compared					651:658	compared	651:658	compared with that of the physical mixtures	651:693	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	3	51	theme	physical	677:684	arg1	mixtures					686:693	the physical mixtures	673:693	the physical mixtures	673:693	Physicochemical analysis proved that ZIP presented at amorphous state in the spray-dried microparticles and the dissolution rate of ZIP from the Soluplus®-ZIP composite microparticles was significantly increased compared with that of the physical mixtures.					
31858315	2	52	theme	spray	357:361	arg1	drying					363:368	spray drying	357:368	spray drying using Soluplus®, an amphiphilic polymer, as the solubility enhancer	357:436	ZIP solid dispersions were produced via spray drying using Soluplus®, an amphiphilic polymer, as the solubility enhancer.					
31858315	5	53	theme	ethyl	904:908	arg1	cellulose					910:918	ethyl cellulose	904:918	ethyl cellulose	904:918	The formulation containing ethyl cellulose provided the most sustained release behaviors.					
31858315	5	54	theme	most	933:936	arg1	behaviors					956:964	the most sustained release behaviors	929:964	the most sustained release behaviors	929:964	The formulation containing ethyl cellulose provided the most sustained release behaviors.					
31858315	6	55	from	studies	983:989	arg1	dogs					1001:1004	beagle dogs	994:1004	beagle dogs	994:1004	Pharmacokinetic studies in beagle dogs confirmed that there was no significant difference in oral bioavailability of the microparticles under fasted and fed states, and a prolonged Tmax value was simultaneously achieved compared with the commercial ZIP capsules.					
31858315	1	56	theme	ziprasidone	234:244	arg1	formulation					219:229	a new formulation	213:229	a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release	213:314	The purpose of this study was to develop and evaluate a new formulation of ziprasidone (ZIP) for improved fasted state absorption and sustained drug release.					
30280291	5	0	theme	blood	812:816	arg1	cells					818:822	red blood cells	808:822	red blood cells	808:822	Through the anticoagulant and endothelial cell reperfusion experiments, it can be demonstrated that the heparinized scaffolds absorbed less platelets and red blood cells which can effectively reduce the formation of thrombosis.					
30280291	5	1	theme	endothelial	684:694	arg1	experiments					713:723	the anticoagulant and endothelial cell reperfusion experiments	662:723	the anticoagulant and endothelial cell reperfusion experiments	662:723	Through the anticoagulant and endothelial cell reperfusion experiments, it can be demonstrated that the heparinized scaffolds absorbed less platelets and red blood cells which can effectively reduce the formation of thrombosis.					
30280291	7	2	theme	better	1158:1163	arg1	conditions					1165:1174	the better conditions	1154:1174	the better conditions for neovascularization	1154:1197	Taken together, our results reveal that the whole kidney can be modified by CBP-heparin composite to reduce the thrombosis and provide the better conditions for neovascularization.					
30280291	5	3	theme	cell	696:699	arg1	experiments					713:723	the anticoagulant and endothelial cell reperfusion experiments	662:723	the anticoagulant and endothelial cell reperfusion experiments	662:723	Through the anticoagulant and endothelial cell reperfusion experiments, it can be demonstrated that the heparinized scaffolds absorbed less platelets and red blood cells which can effectively reduce the formation of thrombosis.					
30280291	1	4	theme	tissue	182:187	arg1	technology					201:210	tissue engineering technology	182:210	tissue engineering technology	182:210	In recent years, rapid development of tissue engineering technology provides possibilities for the construction of artificial tissues or organs.					
30280291	4	5	theme	scaffold	608:615	arg1	part					585:588	the collagen part	572:588	the collagen part of decellularized scaffold with collagen-binding peptide (CBP)	572:651	In this study, heparin was immobilized to the collagen part of decellularized scaffold with collagen-binding peptide (CBP).					
30280291	0	6	theme	inducing	113:120	arg1	reendothelialization					122:141	inducing reendothelialization	113:141	inducing reendothelialization	113:141	Immobilization of heparin on decellularized kidney scaffold to construct microenvironment for antithrombosis and inducing reendothelialization.					
30280291	0	7	from	Immobilization	0:13	arg1	scaffold					51:58	decellularized kidney scaffold	29:58	decellularized kidney scaffold	29:58	Immobilization of heparin on decellularized kidney scaffold to construct microenvironment for antithrombosis and inducing reendothelialization.					
30280291	5	8	theme	reperfusion	701:711	arg1	experiments					713:723	the anticoagulant and endothelial cell reperfusion experiments	662:723	the anticoagulant and endothelial cell reperfusion experiments	662:723	Through the anticoagulant and endothelial cell reperfusion experiments, it can be demonstrated that the heparinized scaffolds absorbed less platelets and red blood cells which can effectively reduce the formation of thrombosis.					
30280291	6	9	theme	cells	945:949	arg1	adhesion					921:928	long-term adhesion	911:928	long-term adhesion of endothelial cells which is important for the formation of subsequent vascularization	911:1016	Moreover, it is conducive to long-term adhesion of endothelial cells which is important for the formation of subsequent vascularization.					
30280291	7	10	mod	modified	1083:1090	arg3	composite					1107:1115	CBP-heparin composite	1095:1115	CBP-heparin composite	1095:1115	Taken together, our results reveal that the whole kidney can be modified by CBP-heparin composite to reduce the thrombosis and provide the better conditions for neovascularization.					
30280291	7	10	mod	modified	1083:1090	arg1	kidney					1069:1074	the whole kidney	1059:1074	the whole kidney	1059:1074	Taken together, our results reveal that the whole kidney can be modified by CBP-heparin composite to reduce the thrombosis and provide the better conditions for neovascularization.					
30280291	4	11	theme	collagen-binding	622:637	arg1	CBP					648:650	CBP	648:650	CBP	648:650	In this study, heparin was immobilized to the collagen part of decellularized scaffold with collagen-binding peptide (CBP).					
30280291	4	11	theme	collagen-binding	622:637	arg1	peptide					639:645	collagen-binding peptide	622:645	collagen-binding peptide (CBP)	622:651	In this study, heparin was immobilized to the collagen part of decellularized scaffold with collagen-binding peptide (CBP).					
30280291	6	12	theme	endothelial	933:943	arg1	cells					945:949	endothelial cells	933:949	endothelial cells	933:949	Moreover, it is conducive to long-term adhesion of endothelial cells which is important for the formation of subsequent vascularization.					
30280291	2	13	theme	kidneys	319:325	arg1	construction					292:303	construction	292:303	construction of engineered kidneys	292:325	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	1	14	theme	engineering	189:199	arg1	technology					201:210	tissue engineering technology	182:210	tissue engineering technology	182:210	In recent years, rapid development of tissue engineering technology provides possibilities for the construction of artificial tissues or organs.					
30280291	2	15	theme	engineered	308:317	arg1	kidneys					319:325	engineered kidneys	308:325	engineered kidneys	308:325	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	1	16	theme	technology	201:210	arg1	development					167:177	rapid development	161:177	rapid development of tissue engineering technology	161:210	In recent years, rapid development of tissue engineering technology provides possibilities for the construction of artificial tissues or organs.					
30280291	5	17	theme	anticoagulant	666:678	arg1	experiments					713:723	the anticoagulant and endothelial cell reperfusion experiments	662:723	the anticoagulant and endothelial cell reperfusion experiments	662:723	Through the anticoagulant and endothelial cell reperfusion experiments, it can be demonstrated that the heparinized scaffolds absorbed less platelets and red blood cells which can effectively reduce the formation of thrombosis.					
30280291	2	18	theme	extracellular	367:379	arg1	ECM					389:391	ECM	389:391	ECM	389:391	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	2	18	theme	extracellular	367:379	arg1	matrix					381:386	native decellularized extracellular matrix	345:386	native decellularized extracellular matrix (ECM)	345:392	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	2	18	theme	extracellular	367:379	arg1	scaffolds					401:409	the scaffolds	397:409	the scaffolds to recellularization	397:430	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	3	19	theme	great	464:468	arg1	issue					470:474	a great issue	462:474	a great issue that hinders the progress of transplantation in vivo	462:527	However, thrombosis has been a great issue that hinders the progress of transplantation in vivo.					
30280291	3	19	theme	great	464:468	arg1	thrombosis					442:451	thrombosis	442:451	thrombosis	442:451	However, thrombosis has been a great issue that hinders the progress of transplantation in vivo.					
30280291	2	20	theme	decellularized	352:365	arg1	ECM					389:391	ECM	389:391	ECM	389:391	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	2	20	theme	decellularized	352:365	arg1	matrix					381:386	native decellularized extracellular matrix	345:386	native decellularized extracellular matrix (ECM)	345:392	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	2	20	theme	decellularized	352:365	arg1	scaffolds					401:409	the scaffolds	397:409	the scaffolds to recellularization	397:430	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	4	21	theme	collagen	576:583	arg1	part					585:588	the collagen part	572:588	the collagen part of decellularized scaffold with collagen-binding peptide (CBP)	572:651	In this study, heparin was immobilized to the collagen part of decellularized scaffold with collagen-binding peptide (CBP).					
30280291	2	22	theme	native	345:350	arg1	ECM					389:391	ECM	389:391	ECM	389:391	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	2	22	theme	native	345:350	arg1	matrix					381:386	native decellularized extracellular matrix	345:386	native decellularized extracellular matrix (ECM)	345:392	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	2	22	theme	native	345:350	arg1	scaffolds					401:409	the scaffolds	397:409	the scaffolds to recellularization	397:430	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	0	23	theme	heparin	18:24	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of heparin on decellularized kidney scaffold	0:58	Immobilization of heparin on decellularized kidney scaffold to construct microenvironment for antithrombosis and inducing reendothelialization.					
30280291	4	24	theme	decellularized	593:606	arg1	scaffold					608:615	decellularized scaffold	593:615	decellularized scaffold with collagen-binding peptide (CBP)	593:651	In this study, heparin was immobilized to the collagen part of decellularized scaffold with collagen-binding peptide (CBP).					
30280291	6	25	theme	long-term	911:919	arg1	adhesion					921:928	long-term adhesion	911:928	long-term adhesion of endothelial cells which is important for the formation of subsequent vascularization	911:1016	Moreover, it is conducive to long-term adhesion of endothelial cells which is important for the formation of subsequent vascularization.					
30280291	3	26	theme	transplantation	505:519	arg1	progress					493:500	the progress	489:500	the progress of transplantation	489:519	However, thrombosis has been a great issue that hinders the progress of transplantation in vivo.					
30280291	0	27	theme	kidney	44:49	arg1	scaffold					51:58	decellularized kidney scaffold	29:58	decellularized kidney scaffold	29:58	Immobilization of heparin on decellularized kidney scaffold to construct microenvironment for antithrombosis and inducing reendothelialization.					
30280291	6	28	theme	vascularization	1002:1016	arg1	formation					978:986	the formation	974:986	the formation of subsequent vascularization	974:1016	Moreover, it is conducive to long-term adhesion of endothelial cells which is important for the formation of subsequent vascularization.					
30280291	7	29	theme	CBP-heparin	1095:1105	arg1	composite					1107:1115	CBP-heparin composite	1095:1115	CBP-heparin composite	1095:1115	Taken together, our results reveal that the whole kidney can be modified by CBP-heparin composite to reduce the thrombosis and provide the better conditions for neovascularization.					
30280291	0	30	theme	decellularized	29:42	arg1	scaffold					51:58	decellularized kidney scaffold	29:58	decellularized kidney scaffold	29:58	Immobilization of heparin on decellularized kidney scaffold to construct microenvironment for antithrombosis and inducing reendothelialization.					
30280291	6	31	theme	subsequent	991:1000	arg1	vascularization					1002:1016	subsequent vascularization	991:1016	subsequent vascularization	991:1016	Moreover, it is conducive to long-term adhesion of endothelial cells which is important for the formation of subsequent vascularization.					
30280291	5	32	theme	heparinized	758:768	arg1	scaffolds					770:778	the heparinized scaffolds	754:778	the heparinized scaffolds	754:778	Through the anticoagulant and endothelial cell reperfusion experiments, it can be demonstrated that the heparinized scaffolds absorbed less platelets and red blood cells which can effectively reduce the formation of thrombosis.					
30280291	7	33	theme	whole	1063:1067	arg1	kidney					1069:1074	the whole kidney	1059:1074	the whole kidney	1059:1074	Taken together, our results reveal that the whole kidney can be modified by CBP-heparin composite to reduce the thrombosis and provide the better conditions for neovascularization.					
30280291	1	34	theme	recent	147:152	arg1	years					154:158	recent years	147:158	recent years	147:158	In recent years, rapid development of tissue engineering technology provides possibilities for the construction of artificial tissues or organs.					
30280291	2	35	used	used	340:343	arg2	researchers					328:338	researchers	328:338	researchers	328:338	In construction of engineered kidneys, researchers used native decellularized extracellular matrix (ECM) as the scaffolds to recellularization.					
30280291	1	36	theme	artificial	259:268	arg1	tissues					270:276	artificial tissues	259:276	artificial tissues	259:276	In recent years, rapid development of tissue engineering technology provides possibilities for the construction of artificial tissues or organs.					
30280291	5	37	theme	thrombosis	870:879	arg1	formation					857:865	the formation	853:865	the formation of thrombosis	853:879	Through the anticoagulant and endothelial cell reperfusion experiments, it can be demonstrated that the heparinized scaffolds absorbed less platelets and red blood cells which can effectively reduce the formation of thrombosis.					
30280291	1	38	theme	tissues	270:276	arg1	construction					243:254	the construction	239:254	the construction of artificial tissues or organs	239:286	In recent years, rapid development of tissue engineering technology provides possibilities for the construction of artificial tissues or organs.					
30280291	1	39	theme	rapid	161:165	arg1	development					167:177	rapid development	161:177	rapid development of tissue engineering technology	161:210	In recent years, rapid development of tissue engineering technology provides possibilities for the construction of artificial tissues or organs.					
30280291	5	40	theme	red	808:810	arg1	cells					818:822	red blood cells	808:822	red blood cells	808:822	Through the anticoagulant and endothelial cell reperfusion experiments, it can be demonstrated that the heparinized scaffolds absorbed less platelets and red blood cells which can effectively reduce the formation of thrombosis.					
30280291	4	41	with	scaffold	608:615	arg1	CBP					648:650	CBP	648:650	CBP	648:650	In this study, heparin was immobilized to the collagen part of decellularized scaffold with collagen-binding peptide (CBP).					
30280291	4	41	with	scaffold	608:615	arg1	peptide					639:645	collagen-binding peptide	622:645	collagen-binding peptide (CBP)	622:651	In this study, heparin was immobilized to the collagen part of decellularized scaffold with collagen-binding peptide (CBP).					
30280291	1	42	theme	organs	281:286	arg1	construction					243:254	the construction	239:254	the construction of artificial tissues or organs	239:286	In recent years, rapid development of tissue engineering technology provides possibilities for the construction of artificial tissues or organs.					
31853645	15	0	theme	liquid/chitosan	1759:1773	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	0	theme	liquid/chitosan	1759:1773	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	2	1	theme	CL	351:352	arg1	assay					355:359	a chemiluminescencc (CL) assay	330:359	a chemiluminescencc (CL) assay for tetracycline	330:376	It was used in a chemiluminescencc (CL) assay for tetracycline.					
31853645	1	2	theme	graphene	285:292	arg1	MGO					309:311	IL/Chit@MGO	301:311	IL/Chit@MGO	301:311	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	1	2	theme	graphene	285:292	arg1	oxide					294:298	magnetic nanoparticle-modified graphene oxide	254:298	magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO)	254:312	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	15	3	theme	bifunctionalized	1736:1751	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	3	theme	bifunctionalized	1736:1751	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	6	4	theme	excellent	901:909	arg1	capability					919:928	excellent loading capability	901:928	excellent loading capability for TC-Apt	901:939	The IL/Chit@MGO is found to possess excellent loading capability for TC-Apt.					
31853645	15	5	theme	TC-Apt	1728:1733	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	5	theme	TC-Apt	1728:1733	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	14	6	dep	%	1587:1587	arg1	to					1579:1580	to	1579:1580	to	1579:1580	The apparent recoveries are 98.0% to 101.3% for the milk sample and 97.0% to 102.2% for the water sample.					
31853645	3	7	theme	sample	563:568	arg1	magnetometry					570:581	vibrating sample magnetometry	553:581	vibrating sample magnetometry	553:581	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	7	theme	sample	563:568	arg1	spectroscopy					442:453	spectroscopy	442:453	spectroscopy	442:453	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	11	8	theme	pM	1350:1351	arg1	range					1377:1381	the 0.16 pM to 2.0 nM concentration range	1341:1381	the 0.16 pM to 2.0 nM concentration range	1341:1381	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	7	9	from	charge	1004:1009	arg1	surface					1018:1024	the surface	1014:1024	the surface of IL/Chit@MGO	1014:1039	This is attributed to the large specific surface and abundant charge on the surface of IL/Chit@MGO.					
31853645	11	10	theme	425 nm	1312:1317	arg1	wavelength					1298:1307	the emission wavelength	1285:1307	the emission wavelength of 425 nm	1285:1317	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	13	11	theme	tetracycline	1500:1511	arg1	detection					1513:1521	tetracycline detection	1500:1521	tetracycline detection in practical samples	1500:1542	The assay was applied to tetracycline detection in practical samples.					
31853645	0	12	theme	aptamer-based	117:129	arg1	assay					131:135	aptamer-based assay	117:135	aptamer-based assay of tetracycline	117:151	A nanocomposite prepared from bifunctionalized ionic liquid, chitosan, graphene oxide and magnetic nanoparticles for aptamer-based assay of tetracycline by chemiluminescence.					
31853645	3	13	theme	adsorption-desorption	521:541	arg1	spectroscopy					442:453	spectroscopy	442:453	spectroscopy	442:453	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	13	theme	adsorption-desorption	521:541	arg1	isotherm					543:550	nitrogen adsorption-desorption isotherm	512:550	nitrogen adsorption-desorption isotherm	512:550	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	1	14	theme	bifunctionalized	211:226	arg1	chitosan					242:249	a bifunctionalized ionic liquid, chitosan	209:249	chitosan	242:249	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	15	15	theme	IL/Chit	1800:1806	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	15	theme	IL/Chit	1800:1806	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	1	16	theme	liquid	234:239	arg1	chitosan					242:249	a bifunctionalized ionic liquid, chitosan	209:249	chitosan	242:249	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	4	17	theme	amplification	747:759	arg1	component					761:769	a signal amplification component	738:769	a signal amplification component	738:769	Subsequently, a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component were modified on IL/Chit@MGO.					
31853645	15	18	theme	Graphical	1651:1659	arg1	G-DNAzyme					1691:1699	G-DNAzyme	1691:1699	G-DNAzyme	1691:1699	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	18	theme	Graphical	1651:1659	arg1	DNAzyme					1682:1688	Graphical abstractG-quadruplex DNAzyme	1651:1688	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme)	1651:1700	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	14	19	dep	%	1627:1627	arg1	to					1619:1620	to	1619:1620	to	1619:1620	The apparent recoveries are 98.0% to 101.3% for the milk sample and 97.0% to 102.2% for the water sample.					
31853645	7	20	theme	MGO	1037:1039	arg1	surface					1018:1024	the surface	1014:1024	the surface of IL/Chit@MGO	1014:1039	This is attributed to the large specific surface and abundant charge on the surface of IL/Chit@MGO.					
31853645	10	21	theme	CL	1221:1222	arg1	system					1224:1229	luminol-H2O2 CL system	1208:1229	luminol-H2O2 CL system	1208:1229	The latter catalyzes the CL of luminol-H2O2 CL system at pH 7.4.					
31853645	1	22	from	chitosan	242:249	arg1	MGO					309:311	IL/Chit@MGO	301:311	IL/Chit@MGO	301:311	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	1	22	from	chitosan	242:249	arg1	oxide					294:298	magnetic nanoparticle-modified graphene oxide	254:298	magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO)	254:312	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	0	23	theme	graphene	71:78	arg1	oxide					80:84	graphene oxide	71:84	graphene oxide	71:84	A nanocomposite prepared from bifunctionalized ionic liquid, chitosan, graphene oxide and magnetic nanoparticles for aptamer-based assay of tetracycline by chemiluminescence.					
31853645	7	24	theme	specific	974:981	arg1	surface					983:989	the large specific surface and abundant charge	964:1009	surface	983:989	This is attributed to the large specific surface and abundant charge on the surface of IL/Chit@MGO.					
31853645	8	25	used	used	1060:1063	arg2	composite					1046:1054	The composite	1042:1054	The composite	1042:1054	The composite was used to construct a CL assay for tetracycline.					
31853645	11	26	theme	optimized	1248:1256	arg1	conditions					1258:1267	optimized conditions	1248:1267	optimized conditions	1248:1267	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	4	27	theme	@	795:795	arg1	MGO					796:798	IL/Chit@MGO	788:798	IL/Chit@MGO	788:798	Subsequently, a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component were modified on IL/Chit@MGO.					
31853645	15	28	theme	aptamer	1719:1725	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	28	theme	aptamer	1719:1725	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	11	29	theme	blue	1274:1277	arg1	CL					1279:1280	the blue CL	1270:1280	the blue CL at the emission wavelength of 425 nm	1270:1317	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	11	30	theme	detection	1392:1400	arg1	limit					1402:1406	the detection limit	1388:1406	the detection limit	1388:1406	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	11	30	theme	detection	1392:1400	arg1	fM					1414:1415	21 fM	1411:1415	21 fM (at 3σ)	1411:1423	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	5	31	theme	@	846:846	arg1	MGO					847:849	the bifunctional G-DNAzyme/TC-Apt/IL/Chit@MGO	805:849	the bifunctional G-DNAzyme/TC-Apt/IL/Chit@MGO	805:849	So, the bifunctional G-DNAzyme/TC-Apt/IL/Chit@MGO was prepared.					
31853645	1	32	theme	IL/Chit	301:307	arg1	MGO					309:311	IL/Chit@MGO	301:311	IL/Chit@MGO	301:311	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	1	32	theme	IL/Chit	301:307	arg1	oxide					294:298	magnetic nanoparticle-modified graphene oxide	254:298	magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO)	254:312	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	3	33	theme	scanning	456:463	arg1	spectroscopy					442:453	spectroscopy	442:453	spectroscopy	442:453	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	33	theme	scanning	456:463	arg1	microscopy					474:483	scanning electron microscopy	456:483	scanning electron microscopy	456:483	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	8	34	theme	CL	1080:1081	arg1	assay					1083:1087	a CL assay	1078:1087	a CL assay for tetracycline	1078:1104	The composite was used to construct a CL assay for tetracycline.					
31853645	5	35	theme	bifunctional	809:820	arg1	MGO					847:849	the bifunctional G-DNAzyme/TC-Apt/IL/Chit@MGO	805:849	the bifunctional G-DNAzyme/TC-Apt/IL/Chit@MGO	805:849	So, the bifunctional G-DNAzyme/TC-Apt/IL/Chit@MGO was prepared.					
31853645	6	36	theme	IL/Chit	869:875	arg1	MGO					877:879	The IL/Chit@MGO	865:879	The IL/Chit@MGO	865:879	The IL/Chit@MGO is found to possess excellent loading capability for TC-Apt.					
31853645	13	37	from	detection	1513:1521	arg1	samples					1536:1542	practical samples	1526:1542	practical samples	1526:1542	The assay was applied to tetracycline detection in practical samples.					
31853645	3	38	theme	X-ray	486:490	arg1	diffraction					499:509	X-ray powder diffraction	486:509	X-ray powder diffraction	486:509	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	38	theme	X-ray	486:490	arg1	spectroscopy					442:453	spectroscopy	442:453	spectroscopy	442:453	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	15	39	theme	@	1774:1774	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	39	theme	@	1774:1774	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	40	theme	ionic	1753:1757	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	40	theme	ionic	1753:1757	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	3	41	theme	nitrogen	512:519	arg1	spectroscopy					442:453	spectroscopy	442:453	spectroscopy	442:453	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	41	theme	nitrogen	512:519	arg1	isotherm					543:550	nitrogen adsorption-desorption isotherm	512:550	nitrogen adsorption-desorption isotherm	512:550	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	1	42	theme	nanoparticle-modified	263:283	arg1	MGO					309:311	IL/Chit@MGO	301:311	IL/Chit@MGO	301:311	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	1	42	theme	nanoparticle-modified	263:283	arg1	oxide					294:298	magnetic nanoparticle-modified graphene oxide	254:298	magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO)	254:312	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	9	43	theme	G-DNAzyme	1166:1174	arg1	release					1151:1157	the release	1147:1157	the release of the G-DNAzyme	1147:1174	Tetracycline binds to TC-Apt and causes the release of the G-DNAzyme.					
31853645	13	44	theme	practical	1526:1534	arg1	samples					1536:1542	practical samples	1526:1542	practical samples	1526:1542	The assay was applied to tetracycline detection in practical samples.					
31853645	6	45	theme	loading	911:917	arg1	capability					919:928	excellent loading capability	901:928	excellent loading capability for TC-Apt	901:939	The IL/Chit@MGO is found to possess excellent loading capability for TC-Apt.					
31853645	4	46	theme	tetracycline-binding	620:639	arg1	TC-Apt					650:655	TC-Apt	650:655	TC-Apt	650:655	Subsequently, a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component were modified on IL/Chit@MGO.					
31853645	4	46	theme	tetracycline-binding	620:639	arg1	aptamer					641:647	a tetracycline-binding aptamer	618:647	a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component	618:769	Subsequently, a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component were modified on IL/Chit@MGO.					
31853645	11	47	theme	concentration	1363:1375	arg1	range					1377:1381	the 0.16 pM to 2.0 nM concentration range	1341:1381	the 0.16 pM to 2.0 nM concentration range	1341:1381	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	3	48	theme	vibrating	553:561	arg1	magnetometry					570:581	vibrating sample magnetometry	553:581	vibrating sample magnetometry	553:581	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	48	theme	vibrating	553:561	arg1	spectroscopy					442:453	spectroscopy	442:453	spectroscopy	442:453	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	4	49	theme	G-quadruplex	695:706	arg1	DNAzyme					708:714	G-quadruplex DNAzyme	695:714	G-quadruplex DNAzyme	695:714	Subsequently, a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component were modified on IL/Chit@MGO.					
31853645	11	50	theme	emission	1289:1296	arg1	wavelength					1298:1307	the emission wavelength	1285:1307	the emission wavelength of 425 nm	1285:1317	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	3	51	theme	zeta	587:590	arg1	spectroscopy					442:453	spectroscopy	442:453	spectroscopy	442:453	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	51	theme	zeta	587:590	arg1	potentials					592:601	zeta potentials	587:601	zeta potentials	587:601	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	6	52	contain	possess	893:899	arg1	MGO					877:879	The IL/Chit@MGO	865:879	The IL/Chit@MGO	865:879	The IL/Chit@MGO is found to possess excellent loading capability for TC-Apt.					
31853645	6	52	contain	possess	893:899	arg2	capability					919:928	excellent loading capability	901:928	excellent loading capability for TC-Apt	901:939	The IL/Chit@MGO is found to possess excellent loading capability for TC-Apt.					
31853645	0	53	theme	tetracycline	140:151	arg1	assay					131:135	aptamer-based assay	117:135	aptamer-based assay of tetracycline	117:151	A nanocomposite prepared from bifunctionalized ionic liquid, chitosan, graphene oxide and magnetic nanoparticles for aptamer-based assay of tetracycline by chemiluminescence.					
31853645	14	54	theme	%	1617:1617	arg1	%					1627:1627	97.0% to 102.2%	1613:1627	97.0% to 102.2% for the water sample	1613:1648	The apparent recoveries are 98.0% to 101.3% for the milk sample and 97.0% to 102.2% for the water sample.					
31853645	16	55	used	used	1849:1852	arg2	nanocomposite					1831:1843	The nanocomposite	1827:1843	The nanocomposite	1827:1843	The nanocomposite was used to construct a chemiluminescence (CL) assay for tetracycline.					
31853645	1	56	theme	ionic	228:232	arg1	chitosan					242:249	a bifunctionalized ionic liquid, chitosan	209:249	chitosan	242:249	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	15	57	theme	@	1807:1807	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	57	theme	@	1807:1807	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	4	58	theme	recognition	670:680	arg1	element					682:688	a recognition element	668:688	a recognition element	668:688	Subsequently, a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component were modified on IL/Chit@MGO.					
31853645	4	58	theme	recognition	670:680	arg1	G-DNAzyme					717:725	G-DNAzyme	717:725	G-DNAzyme	717:725	Subsequently, a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component were modified on IL/Chit@MGO.					
31853645	15	59	theme	graphene	1784:1791	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	59	theme	graphene	1784:1791	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	7	60	theme	IL/Chit	1029:1035	arg1	MGO					1037:1039	IL/Chit@MGO	1029:1039	IL/Chit@MGO	1029:1039	This is attributed to the large specific surface and abundant charge on the surface of IL/Chit@MGO.					
31853645	11	61	dep	range	1377:1381	arg1	to					1353:1354	to	1353:1354	to	1353:1354	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	10	62	theme	system	1224:1229	arg1	CL					1202:1203	the CL	1198:1203	the CL of luminol-H2O2 CL system	1198:1229	The latter catalyzes the CL of luminol-H2O2 CL system at pH 7.4.					
31853645	7	63	theme	@	1036:1036	arg1	MGO					1037:1039	IL/Chit@MGO	1029:1039	IL/Chit@MGO	1029:1039	This is attributed to the large specific surface and abundant charge on the surface of IL/Chit@MGO.					
31853645	14	64	theme	milk	1597:1600	arg1	sample					1602:1607	the milk sample	1593:1607	the milk sample	1593:1607	The apparent recoveries are 98.0% to 101.3% for the milk sample and 97.0% to 102.2% for the water sample.					
31853645	4	65	theme	signal	740:745	arg1	component					761:769	a signal amplification component	738:769	a signal amplification component	738:769	Subsequently, a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component were modified on IL/Chit@MGO.					
31853645	10	66	theme	luminol-H2O2	1208:1219	arg1	system					1224:1229	luminol-H2O2 CL system	1208:1229	luminol-H2O2 CL system	1208:1229	The latter catalyzes the CL of luminol-H2O2 CL system at pH 7.4.					
31853645	7	67	theme	abundant	995:1002	arg1	charge					1004:1009	the large specific surface and abundant charge	964:1009	charge	1004:1009	This is attributed to the large specific surface and abundant charge on the surface of IL/Chit@MGO.					
31853645	7	68	from	surface	983:989	arg1	surface					1018:1024	the surface	1014:1024	the surface of IL/Chit@MGO	1014:1039	This is attributed to the large specific surface and abundant charge on the surface of IL/Chit@MGO.					
31853645	0	69	theme	magnetic	90:97	arg1	nanoparticles					99:111	magnetic nanoparticles	90:111	magnetic nanoparticles	90:111	A nanocomposite prepared from bifunctionalized ionic liquid, chitosan, graphene oxide and magnetic nanoparticles for aptamer-based assay of tetracycline by chemiluminescence.					
31853645	14	70	theme	%	1577:1577	arg1	recoveries					1558:1567	The apparent recoveries	1545:1567	The apparent recoveries	1545:1567	The apparent recoveries are 98.0% to 101.3% for the milk sample and 97.0% to 102.2% for the water sample.					
31853645	14	70	theme	%	1577:1577	arg1	%					1587:1587	98.0% to 101.3%	1573:1587	98.0% to 101.3% for the milk sample	1573:1607	The apparent recoveries are 98.0% to 101.3% for the milk sample and 97.0% to 102.2% for the water sample.					
31853645	16	71	theme	chemiluminescence	1869:1885	arg1	assay					1892:1896	a chemiluminescence (CL) assay	1867:1896	a chemiluminescence (CL) assay for tetracycline	1867:1913	The nanocomposite was used to construct a chemiluminescence (CL) assay for tetracycline.					
31853645	4	72	theme	IL/Chit	788:794	arg1	MGO					796:798	IL/Chit@MGO	788:798	IL/Chit@MGO	788:798	Subsequently, a tetracycline-binding aptamer (TC-Apt) acting as a recognition element, and G-quadruplex DNAzyme (G-DNAzyme) acting as a signal amplification component were modified on IL/Chit@MGO.					
31853645	15	73	theme	tetracycline	1706:1717	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	73	theme	tetracycline	1706:1717	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	11	74	from	wavelength	1298:1307	arg1	CL					1279:1280	the blue CL	1270:1280	the blue CL at the emission wavelength of 425 nm	1270:1317	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	7	75	theme	large	968:972	arg1	surface					983:989	the large specific surface and abundant charge	964:1009	surface	983:989	This is attributed to the large specific surface and abundant charge on the surface of IL/Chit@MGO.					
31853645	16	76	theme	CL	1888:1889	arg1	assay					1892:1896	a chemiluminescence (CL) assay	1867:1896	a chemiluminescence (CL) assay for tetracycline	1867:1913	The nanocomposite was used to construct a chemiluminescence (CL) assay for tetracycline.					
31853645	5	77	theme	G-DNAzyme/TC-Apt/IL/Chit	822:845	arg1	MGO					847:849	the bifunctional G-DNAzyme/TC-Apt/IL/Chit@MGO	805:849	the bifunctional G-DNAzyme/TC-Apt/IL/Chit@MGO	805:849	So, the bifunctional G-DNAzyme/TC-Apt/IL/Chit@MGO was prepared.					
31853645	14	78	theme	apparent	1549:1556	arg1	recoveries					1558:1567	The apparent recoveries	1545:1567	The apparent recoveries	1545:1567	The apparent recoveries are 98.0% to 101.3% for the milk sample and 97.0% to 102.2% for the water sample.					
31853645	14	78	theme	apparent	1549:1556	arg1	%					1587:1587	98.0% to 101.3%	1573:1587	98.0% to 101.3% for the milk sample	1573:1607	The apparent recoveries are 98.0% to 101.3% for the milk sample and 97.0% to 102.2% for the water sample.					
31853645	11	79	from	3σ	1421:1422	arg1	limit					1402:1406	the detection limit	1388:1406	the detection limit	1388:1406	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	11	79	from	3σ	1421:1422	arg1	fM					1414:1415	21 fM	1411:1415	21 fM (at 3σ)	1411:1423	Under optimized conditions, the blue CL at the emission wavelength of 425 nm increases linearly in the 0.16 pM to 2.0 nM concentration range, and the detection limit is 21 fM (at 3σ).					
31853645	3	80	dep	Fourier	415:421	arg1	transform					423:431	transform	423:431	transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials	423:601	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	1	81	theme	@	308:308	arg1	MGO					309:311	IL/Chit@MGO	301:311	IL/Chit@MGO	301:311	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	1	81	theme	@	308:308	arg1	oxide					294:298	magnetic nanoparticle-modified graphene oxide	254:298	magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO)	254:312	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	15	82	theme	abstractG-quadruplex	1661:1680	arg1	G-DNAzyme					1691:1699	G-DNAzyme	1691:1699	G-DNAzyme	1691:1699	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	82	theme	abstractG-quadruplex	1661:1680	arg1	DNAzyme					1682:1688	Graphical abstractG-quadruplex DNAzyme	1651:1688	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme)	1651:1700	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	3	83	dep	transform	423:431	arg1	infrared					433:440	infrared	433:440	transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials	423:601	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	84	theme	electron	465:472	arg1	spectroscopy					442:453	spectroscopy	442:453	spectroscopy	442:453	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	84	theme	electron	465:472	arg1	microscopy					474:483	scanning electron microscopy	456:483	scanning electron microscopy	456:483	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	6	85	theme	@	876:876	arg1	MGO					877:879	The IL/Chit@MGO	865:879	The IL/Chit@MGO	865:879	The IL/Chit@MGO is found to possess excellent loading capability for TC-Apt.					
31853645	2	86	theme	chemiluminescencc	332:348	arg1	assay					355:359	a chemiluminescencc (CL) assay	330:359	a chemiluminescencc (CL) assay for tetracycline	330:376	It was used in a chemiluminescencc (CL) assay for tetracycline.					
31853645	3	87	theme	powder	492:497	arg1	diffraction					499:509	X-ray powder diffraction	486:509	X-ray powder diffraction	486:509	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	3	87	theme	powder	492:497	arg1	spectroscopy					442:453	spectroscopy	442:453	spectroscopy	442:453	The materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray powder diffraction, nitrogen adsorption-desorption isotherm, vibrating sample magnetometry and zeta potentials.					
31853645	2	88	used	used	322:325	arg2	It					315:316	It	315:316	It	315:316	It was used in a chemiluminescencc (CL) assay for tetracycline.					
31853645	15	89	theme	magnetic	1775:1782	arg1	MGO					1808:1810	IL/Chit@MGO	1800:1810	IL/Chit@MGO	1800:1810	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	15	89	theme	magnetic	1775:1782	arg1	oxide					1793:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide	1706:1797	tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO)	1706:1811	Graphical abstractG-quadruplex DNAzyme (G-DNAzyme) and tetracycline aptamer (TC-Apt) bifunctionalized ionic liquid/chitosan@magnetic graphene oxide (IL/Chit@MGO) was prepared.					
31853645	1	90	theme	magnetic	254:261	arg1	MGO					309:311	IL/Chit@MGO	301:311	IL/Chit@MGO	301:311	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	1	90	theme	magnetic	254:261	arg1	oxide					294:298	magnetic nanoparticle-modified graphene oxide	254:298	magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO)	254:312	A nanocomposite was prepared from a bifunctionalized ionic liquid, chitosan on magnetic nanoparticle-modified graphene oxide (IL/Chit@MGO).					
31853645	14	91	theme	water	1637:1641	arg1	sample					1643:1648	the water sample	1633:1648	the water sample	1633:1648	The apparent recoveries are 98.0% to 101.3% for the milk sample and 97.0% to 102.2% for the water sample.					
29875690	1	0	theme	cancer	365:370	arg1	types					350:354	various types	342:354	various types of human cancer	342:370	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	5	1	theme	cancer	869:874	arg1	tissues					876:882	colorectal cancer tissues	858:882	colorectal cancer tissues	858:882	And β3GnT8, CD147, MMP2, and galectin3 were over-expressed in colorectal cancer tissues.					
29875690	9	2	theme	tumor	1395:1399	arg1	target					1401:1406	a tumor target	1393:1406	a tumor target for the therapy of colorectal cancer	1393:1443	These results suggest that the potential use of β3GnT8 as a tumor target for the therapy of colorectal cancer.					
29875690	4	3	from	immunohistochemistry	743:762	arg1	slices					788:793	90 paraffin-embedded slices	767:793	90 paraffin-embedded slices	767:793	In our study, we detected the expression of β3GnT8, CD147, MMP2, and galectin3 by immunohistochemistry on 90 paraffin-embedded slices.					
29875690	6	4	theme	cells	974:978	arg1	invasion					944:951	invasion	944:951	invasion of colorectal cancer cells	944:978	We found that overexpression of β3GnT8 and β3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of β3GnT8 and β3GnT2 inhibited the invasive activity.					
29875690	4	5	theme	galectin3	730:738	arg1	expression					691:700	the expression	687:700	the expression of β3GnT8, CD147, MMP2, and galectin3	687:738	In our study, we detected the expression of β3GnT8, CD147, MMP2, and galectin3 by immunohistochemistry on 90 paraffin-embedded slices.					
29875690	6	6	theme	cancer	967:972	arg1	cells					974:978	colorectal cancer cells	956:978	colorectal cancer cells	956:978	We found that overexpression of β3GnT8 and β3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of β3GnT8 and β3GnT2 inhibited the invasive activity.					
29875690	8	7	theme	cancer	1318:1323	arg1	invasion					1325:1332	colorectal cancer invasion	1307:1332	colorectal cancer invasion	1307:1332	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	1	8	theme	important	303:311	arg1	effect					313:318	an important effect	300:318	an important effect	300:318	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	7	9	theme	cancer	1173:1178	arg1	cells					1180:1184	colorectal cancer cells	1162:1184	colorectal cancer cells	1162:1184	Mechanistically, β3GnT8 and β3GnT2 regulated the expression of HG-CD147 and the level of polylactosamines in colorectal cancer cells.					
29875690	7	10	theme	polylactosamines	1142:1157	arg1	level					1133:1137	the level	1129:1137	the level of polylactosamines	1129:1157	Mechanistically, β3GnT8 and β3GnT2 regulated the expression of HG-CD147 and the level of polylactosamines in colorectal cancer cells.					
29875690	7	10	theme	polylactosamines	1142:1157	arg1	expression					1102:1111	the expression	1098:1111	the expression of HG-CD147	1098:1123	Mechanistically, β3GnT8 and β3GnT2 regulated the expression of HG-CD147 and the level of polylactosamines in colorectal cancer cells.					
29875690	6	11	theme	β3GnT8	1002:1007	arg1	knockdown					989:997	knockdown	989:997	knockdown of β3GnT8 and β3GnT2	989:1018	We found that overexpression of β3GnT8 and β3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of β3GnT8 and β3GnT2 inhibited the invasive activity.					
29875690	1	12	theme	polylactosamine	185:199	arg1	structures					208:217	polylactosamine glycan structures	185:217	polylactosamine glycan structures	185:217	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	6	13	theme	colorectal	956:965	arg1	cells					974:978	colorectal cancer cells	956:978	colorectal cancer cells	956:978	We found that overexpression of β3GnT8 and β3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of β3GnT8 and β3GnT2 inhibited the invasive activity.					
29875690	1	14	theme	glycan	201:206	arg1	structures					208:217	polylactosamine glycan structures	185:217	polylactosamine glycan structures	185:217	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	2	15	theme	matrix	477:482	arg1	MMPs					504:507	MMPs	504:507	MMPs	504:507	They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine.					
29875690	2	15	theme	matrix	477:482	arg1	metalloproteinases					484:501	matrix metalloproteinases	477:501	matrix metalloproteinases (MMPs)	477:508	They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine.					
29875690	8	16	dep	role	1237:1240	arg1	that					1222:1225	that	1222:1225	that	1222:1225	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	1	17	theme	structures	208:217	arg1	formation					172:180	the formation	168:180	the formation of polylactosamine glycan structures	168:217	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	7	18	theme	colorectal	1162:1171	arg1	cells					1180:1184	colorectal cancer cells	1162:1184	colorectal cancer cells	1162:1184	Mechanistically, β3GnT8 and β3GnT2 regulated the expression of HG-CD147 and the level of polylactosamines in colorectal cancer cells.					
29875690	4	19	theme	CD147	713:717	arg1	expression					691:700	the expression	687:700	the expression of β3GnT8, CD147, MMP2, and galectin3	687:738	In our study, we detected the expression of β3GnT8, CD147, MMP2, and galectin3 by immunohistochemistry on 90 paraffin-embedded slices.					
29875690	6	20	theme	invasive	1034:1041	arg1	activity					1043:1050	the invasive activity	1030:1050	the invasive activity	1030:1050	We found that overexpression of β3GnT8 and β3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of β3GnT8 and β3GnT2 inhibited the invasive activity.					
29875690	0	21	theme	Cancer	27:32	arg1	Invasion					40:47	Colorectal Cancer Cells Invasion	16:47	Colorectal Cancer Cells Invasion	16:47	β3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis.					
29875690	9	22	theme	colorectal	1427:1436	arg1	cancer					1438:1443	colorectal cancer	1427:1443	colorectal cancer	1427:1443	These results suggest that the potential use of β3GnT8 as a tumor target for the therapy of colorectal cancer.					
29875690	0	23	theme	Colorectal	16:25	arg1	Invasion					40:47	Colorectal Cancer Cells Invasion	16:47	Colorectal Cancer Cells Invasion	16:47	β3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis.					
29875690	4	24	theme	MMP2	720:723	arg1	expression					691:700	the expression	687:700	the expression of β3GnT8, CD147, MMP2, and galectin3	687:738	In our study, we detected the expression of β3GnT8, CD147, MMP2, and galectin3 by immunohistochemistry on 90 paraffin-embedded slices.					
29875690	9	25	theme	cancer	1438:1443	arg1	therapy					1416:1422	the therapy	1412:1422	the therapy of colorectal cancer	1412:1443	These results suggest that the potential use of β3GnT8 as a tumor target for the therapy of colorectal cancer.					
29875690	6	26	theme	β3GnT2	928:933	arg1	overexpression					899:912	overexpression	899:912	overexpression of β3GnT8 and β3GnT2	899:933	We found that overexpression of β3GnT8 and β3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of β3GnT8 and β3GnT2 inhibited the invasive activity.					
29875690	8	27	theme	novel	1231:1235	arg1	role					1237:1240	the novel role	1227:1240	the novel role	1227:1240	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	6	28	theme	β3GnT2	1013:1018	arg1	knockdown					989:997	knockdown	989:997	knockdown of β3GnT8 and β3GnT2	989:1018	We found that overexpression of β3GnT8 and β3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of β3GnT8 and β3GnT2 inhibited the invasive activity.					
29875690	0	29	theme	Cells	34:38	arg1	Invasion					40:47	Colorectal Cancer Cells Invasion	16:47	Colorectal Cancer Cells Invasion	16:47	β3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis.					
29875690	3	30	theme	underlying	623:632	arg1	mechanisms					634:643	the underlying mechanisms	619:643	the underlying mechanisms	619:643	However, whether β3GnT8 and β3GnT2 play a role in colorectal cancer and, if so, the underlying mechanisms remain unclear.					
29875690	8	31	from	role	1237:1240	arg1	promotion					1294:1302	promotion	1294:1302	promotion of colorectal cancer invasion	1294:1332	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	1	32	theme	tetra-antennary	245:259	arg1	N-glycan					275:282	tetra-antennary β1-6-branched N-glycan	245:282	tetra-antennary β1-6-branched N-glycan	245:282	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	0	33	theme	CD147/MMP2/Galectin3	53:72	arg1	Axis					74:77	CD147/MMP2/Galectin3 Axis	53:77	CD147/MMP2/Galectin3 Axis	53:77	β3GnT8 Promotes Colorectal Cancer Cells Invasion via CD147/MMP2/Galectin3 Axis.					
29875690	1	34	theme	various	342:348	arg1	types					350:354	various types	342:354	various types of human cancer	342:370	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	3	35	theme	colorectal	589:598	arg1	cancer					600:605	colorectal cancer	589:605	colorectal cancer	589:605	However, whether β3GnT8 and β3GnT2 play a role in colorectal cancer and, if so, the underlying mechanisms remain unclear.					
29875690	8	36	theme	β3GnT8	1273:1278	arg1	role					1237:1240	the novel role	1227:1240	the novel role	1227:1240	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	8	36	theme	β3GnT8	1273:1278	arg1	mechanism					1260:1268	the molecular mechanism	1246:1268	the molecular mechanism	1246:1268	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	2	37	theme	metalloproteinases	484:501	arg1	expression					463:472	the expression	459:472	the expression of matrix metalloproteinases (MMPs)	459:508	They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine.					
29875690	2	37	theme	metalloproteinases	484:501	arg1	polylactosamine					522:536	polylactosamine	522:536	polylactosamine	522:536	They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine.					
29875690	2	37	theme	metalloproteinases	484:501	arg1	CD147					511:515	CD147	511:515	CD147	511:515	They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine.					
29875690	1	38	theme	types	350:354	arg1	progression					327:337	the progression	323:337	the progression of various types of human cancer	323:370	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	1	39	theme	key	141:143	arg1	enzymes					145:151	key enzymes	141:151	key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan	141:282	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	1	39	theme	key	141:143	arg1	β1,3-N-acetylglucosaminyltransferase					80:115	β1,3-N-acetylglucosaminyltransferase	80:115	β1,3-N-acetylglucosaminyltransferase (β3GnT8)	80:124	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	1	39	theme	key	141:143	arg1	β3GnT2					130:135	β3GnT2	130:135	β3GnT2	130:135	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	8	40	theme	β3GnT2	1284:1289	arg1	role					1237:1240	the novel role	1227:1240	the novel role	1227:1240	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	8	40	theme	β3GnT2	1284:1289	arg1	mechanism					1260:1268	the molecular mechanism	1246:1268	the molecular mechanism	1246:1268	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	9	41	theme	potential	1366:1374	arg1	use					1376:1378	the potential use	1362:1378	the potential use of β3GnT8	1362:1388	These results suggest that the potential use of β3GnT8 as a tumor target for the therapy of colorectal cancer.					
29875690	1	42	theme	β1-6-branched	261:273	arg1	N-glycan					275:282	tetra-antennary β1-6-branched N-glycan	245:282	tetra-antennary β1-6-branched N-glycan	245:282	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	8	43	theme	invasion	1325:1332	arg1	promotion					1294:1302	promotion	1294:1302	promotion of colorectal cancer invasion	1294:1332	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	8	44	from	mechanism	1260:1268	arg1	promotion					1294:1302	promotion	1294:1302	promotion of colorectal cancer invasion	1294:1332	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	4	45	theme	β3GnT8	705:710	arg1	expression					691:700	the expression	687:700	the expression of β3GnT8, CD147, MMP2, and galectin3	687:738	In our study, we detected the expression of β3GnT8, CD147, MMP2, and galectin3 by immunohistochemistry on 90 paraffin-embedded slices.					
29875690	8	46	theme	colorectal	1307:1316	arg1	invasion					1325:1332	colorectal cancer invasion	1307:1332	colorectal cancer invasion	1307:1332	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	8	47	theme	molecular	1250:1258	arg1	mechanism					1260:1268	the molecular mechanism	1246:1268	the molecular mechanism	1246:1268	Together, these results illustrate that the novel role and the molecular mechanism of β3GnT8 and β3GnT2 in promotion of colorectal cancer invasion.					
29875690	1	48	contain	has	296:298	arg2	effect					313:318	an important effect	300:318	an important effect	300:318	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	1	48	contain	has	296:298	arg1	it					288:289	it	288:289	it	288:289	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	3	49	dep	play	574:577	arg1	so					615:616	so	615:616	so	615:616	However, whether β3GnT8 and β3GnT2 play a role in colorectal cancer and, if so, the underlying mechanisms remain unclear.					
29875690	2	50	theme	tumor	415:419	arg1	invasion					421:428	tumor invasion	415:428	tumor invasion	415:428	They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine.					
29875690	7	51	theme	HG-CD147	1116:1123	arg1	level					1133:1137	the level	1129:1137	the level of polylactosamines	1129:1157	Mechanistically, β3GnT8 and β3GnT2 regulated the expression of HG-CD147 and the level of polylactosamines in colorectal cancer cells.					
29875690	7	51	theme	HG-CD147	1116:1123	arg1	expression					1102:1111	the expression	1098:1111	the expression of HG-CD147	1098:1123	Mechanistically, β3GnT8 and β3GnT2 regulated the expression of HG-CD147 and the level of polylactosamines in colorectal cancer cells.					
29875690	1	52	theme	human	359:363	arg1	cancer					365:370	human cancer	359:370	human cancer	359:370	β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N-glycan and it also has an important effect on the progression of various types of human cancer.					
29875690	4	53	theme	paraffin-embedded	770:786	arg1	slices					788:793	90 paraffin-embedded slices	767:793	90 paraffin-embedded slices	767:793	In our study, we detected the expression of β3GnT8, CD147, MMP2, and galectin3 by immunohistochemistry on 90 paraffin-embedded slices.					
29875690	5	54	theme	colorectal	858:867	arg1	tissues					876:882	colorectal cancer tissues	858:882	colorectal cancer tissues	858:882	And β3GnT8, CD147, MMP2, and galectin3 were over-expressed in colorectal cancer tissues.					
29875690	9	55	theme	β3GnT8	1383:1388	arg1	use					1376:1378	the potential use	1362:1378	the potential use of β3GnT8	1362:1388	These results suggest that the potential use of β3GnT8 as a tumor target for the therapy of colorectal cancer.					
29875690	6	56	theme	β3GnT8	917:922	arg1	overexpression					899:912	overexpression	899:912	overexpression of β3GnT8 and β3GnT2	899:933	We found that overexpression of β3GnT8 and β3GnT2 promoted invasion of colorectal cancer cells, whereas knockdown of β3GnT8 and β3GnT2 inhibited the invasive activity.					
30844051	8	0	theme	dietary	1605:1611	arg1	treatments					1624:1633	dietary or cooling treatments	1605:1633	dietary or cooling treatments	1605:1633	Intramammary LPS infusion reduced (P < 0.01) milk yield, DMI and feed efficiency regardless of dietary or cooling treatments.					
30844051	11	1	theme	non-cooled	1943:1952	arg1	cows					1954:1957	non-cooled cows	1943:1957	non-cooled cows	1943:1957	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	11	2	contain	had	1959:1961	arg1	cows					1954:1957	non-cooled cows	1943:1957	non-cooled cows	1943:1957	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	11	2	contain	had	1959:1961	arg2	concentrations					2040:2053	lower (P < 0.03) blood concentrations	2017:2053	lower (P < 0.03) blood concentrations of neutrophils and lymphocytes	2017:2084	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	11	2	contain	had	1959:1961	arg2	concentrations					1997:2010	greater (P < 0.05) plasma lactose concentrations	1963:2010	greater (P < 0.05) plasma lactose concentrations	1963:2010	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	9	3	dep	have	1673:1676	arg1	P					1660:1660	P	1660:1660	P = 0.09	1660:1667	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	8	4	theme	P	1545:1545	arg1	<					1547:1547	P < 0.01	1545:1552	P < 0.01	1545:1552	Intramammary LPS infusion reduced (P < 0.01) milk yield, DMI and feed efficiency regardless of dietary or cooling treatments.					
30844051	8	4	theme	P	1545:1545	arg1	yield					1560:1564	milk yield	1555:1564	milk yield	1555:1564	Intramammary LPS infusion reduced (P < 0.01) milk yield, DMI and feed efficiency regardless of dietary or cooling treatments.					
30844051	8	5	theme	Intramammary	1510:1521	arg1	infusion					1527:1534	Intramammary LPS infusion	1510:1534	Intramammary LPS infusion	1510:1534	Intramammary LPS infusion reduced (P < 0.01) milk yield, DMI and feed efficiency regardless of dietary or cooling treatments.					
30844051	2	6	theme	treatments	551:560	arg1	hydroxychloride					680:694	35 mg/kg Zn hydroxychloride	668:694	35 mg/kg Zn hydroxychloride	668:694	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	6	theme	treatments	551:560	arg1	sources					609:615	two sources	605:615	two sources of supplemental Zn	605:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	6	theme	treatments	551:560	arg1	one					539:541	one	539:541	one	539:541	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	6	theme	treatments	551:560	arg1	hydroxychloride					649:663	75 mg/kg Zn hydroxychloride	637:663	75 mg/kg Zn hydroxychloride	637:663	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	6	theme	treatments	551:560	arg1	complex					714:720	40 mg/kg Zn-Met complex	698:720	40 mg/kg Zn-Met complex	698:720	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	6	theme	treatments	551:560	arg1	treatments					551:560	four treatments	546:560	four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC)	546:726	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	5	7	from	quarters	1073:1080	arg1	samples					1055:1061	Individual milk samples	1039:1061	Individual milk samples from both quarters	1039:1080	Individual milk samples from both quarters were collected at -12, -4, 0, 6, 12, 24, 48, 72, 96, 120, 144, and 168 h relative to infusion and analyzed for composition and bovine serum albumin.					
30844051	13	8	contain	have	2287:2290	arg2	migration					2310:2318	greater leukocyte migration	2292:2318	greater leukocyte migration into the mammary gland	2292:2341	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	13	8	contain	have	2287:2290	arg1	cows					2223:2226	noncooled cows	2213:2226	noncooled cows	2213:2226	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	4	9	dep	cows	903:906	arg1	=					930:930	=	930:930	=	930:930	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	2	10	with	treatments	551:560	arg1	arrangement					583:593	a 2×2 factorial arrangement	567:593	a 2×2 factorial arrangement	567:593	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	13	11	theme	leukocyte	2300:2308	arg1	migration					2310:2318	greater leukocyte migration	2292:2318	greater leukocyte migration into the mammary gland	2292:2341	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	10	12	theme	milk	1906:1909	arg1	SCC					1911:1913	milk SCC	1906:1913	milk SCC	1906:1913	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	14	13	theme	Dietary	2414:2420	arg1	source					2438:2443	Dietary supplemental Zn source	2414:2443	Dietary supplemental Zn source	2414:2443	Dietary supplemental Zn source had no impact on measures assessed after intramammary LPS infusion.					
30844051	2	14	theme	Zn-Met	707:712	arg1	complex					714:720	40 mg/kg Zn-Met complex	698:720	40 mg/kg Zn-Met complex	698:720	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	14	theme	Zn-Met	707:712	arg1	treatments					551:560	four treatments	546:560	four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC)	546:726	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	14	theme	Zn-Met	707:712	arg1	sources					609:615	two sources	605:615	two sources of supplemental Zn	605:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	12	15	theme	heat-stressed	2178:2190	arg1	cows					2192:2195	heat-stressed cows	2178:2195	heat-stressed cows	2178:2195	This suggests a greater leukocyte migration into the mammary gland of heat-stressed cows.					
30844051	0	16	theme	hematologic	195:205	arg1	profile1					207:214	hematologic profile1	195:214	hematologic profile1	195:214	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	1	17	theme	intramammary	439:450	arg1	infusion					477:484	intramammary lipopolysaccharide (LPS) infusion	439:484	intramammary lipopolysaccharide (LPS) infusion	439:484	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	13	18	theme	feed	2255:2258	arg1	efficiency					2260:2269	greater feed efficiency	2247:2269	greater feed efficiency	2247:2269	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	2	19	theme	factorial	573:581	arg1	arrangement					583:593	a 2×2 factorial arrangement	567:593	a 2×2 factorial arrangement	567:593	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	20	theme	40	698:699	arg1	mg/kg					701:705	mg/kg	701:705	mg/kg	701:705	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	14	21	theme	intramammary	2486:2497	arg1	infusion					2503:2510	intramammary LPS infusion	2486:2510	intramammary LPS infusion	2486:2510	Dietary supplemental Zn source had no impact on measures assessed after intramammary LPS infusion.					
30844051	6	22	theme	Rectal	1231:1236	arg1	temperature					1238:1248	Rectal temperature	1231:1248	Rectal temperature	1231:1248	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	11	23	theme	P	1972:1972	arg1	<					1974:1974	P < 0.05	1972:1979	P < 0.05	1972:1979	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	0	24	theme	intramammary	113:124	arg1	infusion					145:152	intramammary lipopolysaccharide infusion	113:152	intramammary lipopolysaccharide infusion	113:152	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	2	25	dep	treatments	551:560	arg1	ZMC					723:725	ZMC	723:725	ZMC	723:725	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	25	dep	treatments	551:560	arg1	sources					609:615	two sources	605:615	two sources of supplemental Zn	605:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	25	dep	treatments	551:560	arg1	hydroxychloride					680:694	35 mg/kg Zn hydroxychloride	668:694	35 mg/kg Zn hydroxychloride	668:694	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	25	dep	treatments	551:560	arg1	hydroxychloride					649:663	75 mg/kg Zn hydroxychloride	637:663	75 mg/kg Zn hydroxychloride	637:663	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	25	dep	treatments	551:560	arg1	treatments					551:560	four treatments	546:560	four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC)	546:726	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	11	26	theme	cooled	1930:1935	arg1	cows					1937:1940	cooled cows	1930:1940	cooled cows	1930:1940	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	8	27	theme	feed	1575:1578	arg1	efficiency					1580:1589	feed efficiency	1575:1589	feed efficiency	1575:1589	Intramammary LPS infusion reduced (P < 0.01) milk yield, DMI and feed efficiency regardless of dietary or cooling treatments.					
30844051	12	28	theme	leukocyte	2132:2140	arg1	migration					2142:2150	a greater leukocyte migration	2122:2150	a greater leukocyte migration into the mammary gland of heat-stressed cows	2122:2195	This suggests a greater leukocyte migration into the mammary gland of heat-stressed cows.					
30844051	11	29	theme	greater	1963:1969	arg1	concentrations					1997:2010	greater (P < 0.05) plasma lactose concentrations	1963:2010	greater (P < 0.05) plasma lactose concentrations	1963:2010	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	2	30	theme	Zn	633:634	arg1	sources					609:615	two sources	605:615	two sources of supplemental Zn	605:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	31	theme	Zn	646:647	arg1	sources					609:615	two sources	605:615	two sources of supplemental Zn	605:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	31	theme	Zn	646:647	arg1	hydroxychloride					649:663	75 mg/kg Zn hydroxychloride	637:663	75 mg/kg Zn hydroxychloride	637:663	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	31	theme	Zn	646:647	arg1	treatments					551:560	four treatments	546:560	four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC)	546:726	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	0	32	theme	milk	163:166	arg1	yield					168:172	milk yield	163:172	milk yield	163:172	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	1	33	theme	migration	396:404	arg1	intake					334:339	intake	334:339	intake	334:339	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	1	33	theme	migration	396:404	arg1	yield					347:351	milk yield	342:351	milk yield	342:351	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	1	33	theme	migration	396:404	arg1	rate					378:381	the rate	374:381	the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion	374:484	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	1	33	theme	migration	396:404	arg1	composition					357:367	composition	357:367	composition	357:367	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	14	34	theme	Zn	2435:2436	arg1	source					2438:2443	Dietary supplemental Zn source	2414:2443	Dietary supplemental Zn source	2414:2443	Dietary supplemental Zn source had no impact on measures assessed after intramammary LPS infusion.					
30844051	6	35	theme	blood	1289:1293	arg1	samples					1295:1301	blood samples	1289:1301	blood samples	1289:1301	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	7	36	theme	Complete	1415:1422	arg1	counts					1430:1435	Complete blood counts	1415:1435	Complete blood counts	1415:1435	Complete blood counts were performed on samples collected within the first 24 h post infusion.					
30844051	2	37	theme	Multiparous	487:497	arg1	cows					508:511	Multiparous Holstein cows	487:511	Multiparous Holstein cows (n = 72)	487:520	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	37	theme	Multiparous	487:497	arg1	n					514:514	n = 72	514:519	n = 72	514:519	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	0	38	theme	zinc	60:63	arg1	sources					65:71	different supplemental zinc sources	37:71	different supplemental zinc sources	37:71	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	1	39	theme	cooling	323:329	arg1	effect					266:271	the effect	262:271	the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion	262:484	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	13	40	theme	cooled	2401:2406	arg1	cows					2408:2411	cooled cows	2401:2411	cooled cows	2401:2411	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	11	41	theme	blood	2034:2038	arg1	concentrations					2040:2053	lower (P < 0.03) blood concentrations	2017:2053	lower (P < 0.03) blood concentrations of neutrophils and lymphocytes	2017:2084	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	2	42	theme	mg/kg	671:675	arg1	ZMC					723:725	ZMC	723:725	ZMC	723:725	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	42	theme	mg/kg	671:675	arg1	hydroxychloride					680:694	35 mg/kg Zn hydroxychloride	668:694	35 mg/kg Zn hydroxychloride	668:694	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	42	theme	mg/kg	671:675	arg1	treatments					551:560	four treatments	546:560	four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC)	546:726	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	42	theme	mg/kg	671:675	arg1	sources					609:615	two sources	605:615	two sources of supplemental Zn	605:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	6	43	theme	additional	1351:1360	arg1	sample					1362:1367	an additional sample	1348:1367	an additional sample	1348:1367	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	10	44	theme	somatic	1840:1846	arg1	SCC					1860:1862	SCC	1860:1862	SCC	1860:1862	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	10	44	theme	somatic	1840:1846	arg1	count					1853:1857	milk somatic cell count	1835:1857	milk somatic cell count (SCC)	1835:1863	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	1	45	theme	milk	342:345	arg1	intake					334:339	intake	334:339	intake	334:339	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	1	45	theme	milk	342:345	arg1	yield					347:351	milk yield	342:351	milk yield	342:351	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	4	46	dep	=	930:930	arg1	n					909:909	n	909:909	n = 16	909:914	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	10	47	dep	increased	1814:1822	arg1	<					1827:1827	P < 0.01	1825:1832	P < 0.01	1825:1832	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	6	48	theme	cortisol	1405:1412	arg1	analyses					1381:1388	analyses	1381:1388	analyses of lactose and cortisol	1381:1412	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	8	49	theme	LPS	1523:1525	arg1	infusion					1527:1534	Intramammary LPS infusion	1510:1534	Intramammary LPS infusion	1510:1534	Intramammary LPS infusion reduced (P < 0.01) milk yield, DMI and feed efficiency regardless of dietary or cooling treatments.					
30844051	6	50	theme	lactose	1393:1399	arg1	analyses					1381:1388	analyses	1381:1388	analyses of lactose and cortisol	1381:1412	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	2	51	theme	75	637:638	arg1	mg/kg					640:644	mg/kg	640:644	mg/kg	640:644	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	10	52	contain	had	1880:1882	arg2	impact					1896:1901	no apparent impact	1884:1901	no apparent impact	1884:1901	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	10	52	contain	had	1880:1882	arg1	treatments					1869:1878	treatments	1869:1878	treatments	1869:1878	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	6	53	theme	same	1325:1328	arg1	points					1335:1340	the same time points	1321:1340	the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol	1321:1412	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	3	54	theme	feeding	859:865	arg1	areas					867:871	the freestall and feeding areas	841:871	the freestall and feeding areas	841:871	The cooling system was implemented by the use of fans and misters over the freestall and feeding areas.					
30844051	4	55	theme	rear	1024:1027	arg1	quarters					1029:1036	the left or right rear quarters	1006:1036	the left or right rear quarters	1006:1036	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	11	56	dep	lower	2017:2021	arg1	P					2024:2024	P < 0.03	2024:2031	P < 0.03	2024:2031	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	0	57	theme	lactating	12:20	arg1	cows					28:31	lactating dairy cows	12:31	lactating dairy cows	12:31	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	1	58	theme	dietary	276:282	arg1	source					300:305	dietary supplemental Zn source	276:305	dietary supplemental Zn source	276:305	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	11	59	theme	plasma	1982:1987	arg1	concentrations					1997:2010	greater (P < 0.05) plasma lactose concentrations	1963:2010	greater (P < 0.05) plasma lactose concentrations	1963:2010	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	3	60	theme	freestall	845:853	arg1	areas					867:871	the freestall and feeding areas	841:871	the freestall and feeding areas	841:871	The cooling system was implemented by the use of fans and misters over the freestall and feeding areas.					
30844051	4	61	theme	μg	971:972	arg1	control					994:1000	a saline control	985:1000	a saline control	985:1000	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	4	61	theme	μg	971:972	arg1	infusion					956:963	an infusion	953:963	an infusion of 10 μg of LPS	953:979	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	0	62	theme	cows	28:31	arg1	Response					0:7	Response	0:7	Response of lactating dairy cows	0:31	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	1	63	theme	Zn	297:298	arg1	source					300:305	dietary supplemental Zn source	276:305	dietary supplemental Zn source	276:305	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	4	64	theme	=	911:911	arg1	n					909:909	n	909:909	n = 16	909:914	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	5	65	theme	serum	1216:1220	arg1	albumin					1222:1228	bovine serum albumin	1209:1228	bovine serum albumin	1209:1228	Individual milk samples from both quarters were collected at -12, -4, 0, 6, 12, 24, 48, 72, 96, 120, 144, and 168 h relative to infusion and analyzed for composition and bovine serum albumin.					
30844051	8	66	theme	cooling	1616:1622	arg1	treatments					1624:1633	dietary or cooling treatments	1605:1633	dietary or cooling treatments	1605:1633	Intramammary LPS infusion reduced (P < 0.01) milk yield, DMI and feed efficiency regardless of dietary or cooling treatments.					
30844051	0	67	theme	different	37:45	arg1	sources					65:71	different supplemental zinc sources	37:71	different supplemental zinc sources	37:71	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	2	68	theme	evaporative	749:759	arg1	cooling					761:767	evaporative cooling	749:767	evaporative cooling	749:767	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	6	69	from	h	1374:1374	arg1	sample					1362:1367	an additional sample	1348:1367	an additional sample	1348:1367	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	10	70	theme	P	1825:1825	arg1	<					1827:1827	P < 0.01	1825:1832	P < 0.01	1825:1832	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	13	71	theme	greater	2247:2253	arg1	efficiency					2260:2269	greater feed efficiency	2247:2269	greater feed efficiency	2247:2269	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	12	72	theme	greater	2124:2130	arg1	migration					2142:2150	a greater leukocyte migration	2122:2150	a greater leukocyte migration into the mammary gland of heat-stressed cows	2122:2195	This suggests a greater leukocyte migration into the mammary gland of heat-stressed cows.					
30844051	5	73	theme	milk	1050:1053	arg1	samples					1055:1061	Individual milk samples	1039:1061	Individual milk samples from both quarters	1039:1080	Individual milk samples from both quarters were collected at -12, -4, 0, 6, 12, 24, 48, 72, 96, 120, 144, and 168 h relative to infusion and analyzed for composition and bovine serum albumin.					
30844051	0	74	theme	supplemental	47:58	arg1	sources					65:71	different supplemental zinc sources	37:71	different supplemental zinc sources	37:71	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	4	75	dep	=	911:911	arg1	16					913:914	16	913:914	16	913:914	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	2	76	dep	one	539:541	arg1	each					728:731	each	728:731	each	728:731	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	14	77	theme	LPS	2499:2501	arg1	infusion					2503:2510	intramammary LPS infusion	2486:2510	intramammary LPS infusion	2486:2510	Dietary supplemental Zn source had no impact on measures assessed after intramammary LPS infusion.					
30844051	7	78	theme	first	1484:1488	arg1	h					1493:1493	the first 24 h	1480:1493	the first 24 h post infusion	1480:1507	Complete blood counts were performed on samples collected within the first 24 h post infusion.					
30844051	4	79	theme	±	936:936	arg1	d					941:941	263 ± 63 d	932:941	263 ± 63 d	932:941	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	1	80	theme	mammary	415:421	arg1	gland					423:427	the mammary gland	411:427	the mammary gland	411:427	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	4	81	theme	saline	987:992	arg1	control					994:1000	a saline control	985:1000	a saline control	985:1000	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	14	82	theme	supplemental	2422:2433	arg1	source					2438:2443	Dietary supplemental Zn source	2414:2443	Dietary supplemental Zn source	2414:2443	Dietary supplemental Zn source had no impact on measures assessed after intramammary LPS infusion.					
30844051	4	83	theme	LPS	977:979	arg1	μg					971:972	10 μg	968:972	10 μg of LPS	968:979	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	8	84	theme	milk	1555:1558	arg1	<					1547:1547	P < 0.01	1545:1552	P < 0.01	1545:1552	Intramammary LPS infusion reduced (P < 0.01) milk yield, DMI and feed efficiency regardless of dietary or cooling treatments.					
30844051	8	84	theme	milk	1555:1558	arg1	yield					1560:1564	milk yield	1555:1564	milk yield	1555:1564	Intramammary LPS infusion reduced (P < 0.01) milk yield, DMI and feed efficiency regardless of dietary or cooling treatments.					
30844051	6	85	theme	respiration	1254:1264	arg1	rate					1266:1269	respiration rate	1254:1269	respiration rate	1254:1269	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	2	86	theme	mg/kg	701:705	arg1	complex					714:720	40 mg/kg Zn-Met complex	698:720	40 mg/kg Zn-Met complex	698:720	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	86	theme	mg/kg	701:705	arg1	treatments					551:560	four treatments	546:560	four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC)	546:726	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	86	theme	mg/kg	701:705	arg1	sources					609:615	two sources	605:615	two sources of supplemental Zn	605:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	13	87	theme	LPS	2374:2376	arg1	infusion					2378:2385	intramammary LPS infusion	2361:2385	intramammary LPS infusion compared with cooled cows	2361:2411	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	1	88	from	effect	266:271	arg1	intake					334:339	intake	334:339	intake	334:339	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	1	88	from	effect	266:271	arg1	yield					347:351	milk yield	342:351	milk yield	342:351	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	1	88	from	effect	266:271	arg1	rate					378:381	the rate	374:381	the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion	374:484	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	1	88	from	effect	266:271	arg1	composition					357:367	composition	357:367	composition	357:367	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	1	89	theme	lipopolysaccharide	452:469	arg1	infusion					477:484	intramammary lipopolysaccharide (LPS) infusion	439:484	intramammary lipopolysaccharide (LPS) infusion	439:484	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	2	90	theme	2×2	569:571	arg1	arrangement					583:593	a 2×2 factorial arrangement	567:593	a 2×2 factorial arrangement	567:593	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	10	91	theme	Intramammary	1775:1786	arg1	infusion					1792:1799	Intramammary LPS infusion	1775:1799	Intramammary LPS infusion	1775:1799	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	0	92	theme	lipopolysaccharide	126:143	arg1	infusion					145:152	intramammary lipopolysaccharide infusion	113:152	intramammary lipopolysaccharide infusion	113:152	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	9	93	theme	=	1662:1662	arg1	P					1660:1660	P	1660:1660	P = 0.09	1660:1667	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	13	94	theme	noncooled	2213:2221	arg1	cows					2223:2226	noncooled cows	2213:2226	noncooled cows	2213:2226	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	2	95	theme	=	516:516	arg1	cows					508:511	Multiparous Holstein cows	487:511	Multiparous Holstein cows (n = 72)	487:520	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	95	theme	=	516:516	arg1	n					514:514	n = 72	514:519	n = 72	514:519	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	11	96	theme	lymphocytes	2074:2084	arg1	concentrations					2040:2053	lower (P < 0.03) blood concentrations	2017:2053	lower (P < 0.03) blood concentrations of neutrophils and lymphocytes	2017:2084	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	13	97	theme	mammary	2329:2335	arg1	gland					2337:2341	the mammary gland	2325:2341	the mammary gland	2325:2341	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	2	98	theme	35	668:669	arg1	mg/kg					671:675	mg/kg	671:675	mg/kg	671:675	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	11	99	theme	lactose	1989:1995	arg1	concentrations					1997:2010	greater (P < 0.05) plasma lactose concentrations	1963:2010	greater (P < 0.05) plasma lactose concentrations	1963:2010	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	4	100	theme	left	1010:1013	arg1	quarters					1029:1036	the left or right rear quarters	1006:1036	the left or right rear quarters	1006:1036	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	1	101	theme	leukocyte	386:394	arg1	migration					396:404	leukocyte migration	386:404	leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion	386:484	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	13	102	theme	intramammary	2361:2372	arg1	infusion					2378:2385	intramammary LPS infusion	2361:2385	intramammary LPS infusion compared with cooled cows	2361:2411	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	5	103	dep	relative	1155:1162	arg1	144					1140:1142	144	1140:1142	144	1140:1142	Individual milk samples from both quarters were collected at -12, -4, 0, 6, 12, 24, 48, 72, 96, 120, 144, and 168 h relative to infusion and analyzed for composition and bovine serum albumin.					
30844051	5	103	dep	relative	1155:1162	arg1	168					1149:1151	168	1149:1151	168	1149:1151	Individual milk samples from both quarters were collected at -12, -4, 0, 6, 12, 24, 48, 72, 96, 120, 144, and 168 h relative to infusion and analyzed for composition and bovine serum albumin.					
30844051	10	104	theme	LPS	1788:1790	arg1	infusion					1792:1799	Intramammary LPS infusion	1775:1799	Intramammary LPS infusion	1775:1799	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	10	105	theme	cell	1848:1851	arg1	SCC					1860:1862	SCC	1860:1862	SCC	1860:1862	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	10	105	theme	cell	1848:1851	arg1	count					1853:1857	milk somatic cell count	1835:1857	milk somatic cell count (SCC)	1835:1863	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	11	106	dep	greater	1963:1969	arg1	<					1974:1974	P < 0.05	1972:1979	P < 0.05	1972:1979	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	9	107	from	d	1725:1725	arg1	yield/DMI					1709:1717	=milk yield/DMI	1703:1717	=milk yield/DMI	1703:1717	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	9	107	from	d	1725:1725	arg1	efficiency					1691:1700	greater feed efficiency	1678:1700	greater feed efficiency (=milk yield/DMI) at 1 d	1678:1725	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	7	108	theme	blood	1424:1428	arg1	counts					1430:1435	Complete blood counts	1415:1435	Complete blood counts	1415:1435	Complete blood counts were performed on samples collected within the first 24 h post infusion.					
30844051	2	109	theme	Zn	677:678	arg1	ZMC					723:725	ZMC	723:725	ZMC	723:725	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	109	theme	Zn	677:678	arg1	hydroxychloride					680:694	35 mg/kg Zn hydroxychloride	668:694	35 mg/kg Zn hydroxychloride	668:694	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	109	theme	Zn	677:678	arg1	treatments					551:560	four treatments	546:560	four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC)	546:726	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	109	theme	Zn	677:678	arg1	sources					609:615	two sources	605:615	two sources of supplemental Zn	605:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	3	110	theme	fans	819:822	arg1	use					812:814	the use	808:814	the use of fans and misters	808:834	The cooling system was implemented by the use of fans and misters over the freestall and feeding areas.					
30844051	4	111	theme	experiment	891:900	arg1	day					877:879	day 34	877:882	day 34 of the experiment	877:900	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	11	112	theme	<	2026:2026	arg1	P					2024:2024	P < 0.03	2024:2031	P < 0.03	2024:2031	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	12	113	theme	cows	2192:2195	arg1	gland					2169:2173	the mammary gland	2157:2173	the mammary gland of heat-stressed cows	2157:2195	This suggests a greater leukocyte migration into the mammary gland of heat-stressed cows.					
30844051	2	114	theme	supplemental	620:631	arg1	Zn					633:634	supplemental Zn	620:634	supplemental Zn	620:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	3	115	theme	cooling	774:780	arg1	system					782:787	The cooling system	770:787	The cooling system	770:787	The cooling system was implemented by the use of fans and misters over the freestall and feeding areas.					
30844051	0	116	theme	evaporative	90:100	arg1	cooling					102:108	evaporative cooling	90:108	evaporative cooling to intramammary lipopolysaccharide infusion	90:152	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	12	117	theme	mammary	2161:2167	arg1	gland					2169:2173	the mammary gland	2157:2173	the mammary gland of heat-stressed cows	2157:2195	This suggests a greater leukocyte migration into the mammary gland of heat-stressed cows.					
30844051	11	118	theme	neutrophils	2058:2068	arg1	concentrations					2040:2053	lower (P < 0.03) blood concentrations	2017:2053	lower (P < 0.03) blood concentrations of neutrophils and lymphocytes	2017:2084	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	5	119	theme	bovine	1209:1214	arg1	albumin					1222:1228	bovine serum albumin	1209:1228	bovine serum albumin	1209:1228	Individual milk samples from both quarters were collected at -12, -4, 0, 6, 12, 24, 48, 72, 96, 120, 144, and 168 h relative to infusion and analyzed for composition and bovine serum albumin.					
30844051	10	120	theme	milk	1835:1838	arg1	SCC					1860:1862	SCC	1860:1862	SCC	1860:1862	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	10	120	theme	milk	1835:1838	arg1	count					1853:1857	milk somatic cell count	1835:1857	milk somatic cell count (SCC)	1835:1863	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	9	121	contain	have	1673:1676	arg2	efficiency					1691:1700	greater feed efficiency	1678:1700	greater feed efficiency (=milk yield/DMI) at 1 d	1678:1725	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	9	121	contain	have	1673:1676	arg1	cows					1647:1650	Non-cooled cows	1636:1650	Non-cooled cows	1636:1650	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	9	121	contain	have	1673:1676	arg2	yield/DMI					1709:1717	=milk yield/DMI	1703:1717	=milk yield/DMI	1703:1717	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	2	122	theme	Holstein	499:506	arg1	cows					508:511	Multiparous Holstein cows	487:511	Multiparous Holstein cows (n = 72)	487:520	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	122	theme	Holstein	499:506	arg1	n					514:514	n = 72	514:519	n = 72	514:519	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	4	123	theme	right	1018:1022	arg1	quarters					1029:1036	the left or right rear quarters	1006:1036	the left or right rear quarters	1006:1036	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	6	124	theme	time	1330:1333	arg1	points					1335:1340	the same time points	1321:1340	the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol	1321:1412	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	3	125	theme	misters	828:834	arg1	use					812:814	the use	808:814	the use of fans and misters	808:834	The cooling system was implemented by the use of fans and misters over the freestall and feeding areas.					
30844051	9	126	theme	feed	1686:1689	arg1	yield/DMI					1709:1717	=milk yield/DMI	1703:1717	=milk yield/DMI	1703:1717	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	9	126	theme	feed	1686:1689	arg1	efficiency					1691:1700	greater feed efficiency	1678:1700	greater feed efficiency (=milk yield/DMI) at 1 d	1678:1725	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	0	127	theme	dairy	22:26	arg1	cows					28:31	lactating dairy cows	12:31	lactating dairy cows	12:31	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	4	128	from	days	917:920	arg1	milk					925:928	milk	925:928	milk	925:928	On day 34 of the experiment, cows (n = 16; days in milk = 263 ± 63 d) received an infusion of 10 μg of LPS, or a saline control, in the left or right rear quarters.					
30844051	11	129	theme	lower	2017:2021	arg1	concentrations					2040:2053	lower (P < 0.03) blood concentrations	2017:2053	lower (P < 0.03) blood concentrations of neutrophils and lymphocytes	2017:2084	Compared with cooled cows, non-cooled cows had greater (P < 0.05) plasma lactose concentrations, but lower (P < 0.03) blood concentrations of neutrophils and lymphocytes at 3 h post infusion.					
30844051	1	130	theme	supplemental	284:295	arg1	source					300:305	dietary supplemental Zn source	276:305	dietary supplemental Zn source	276:305	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	9	131	theme	Non-cooled	1636:1645	arg1	cows					1647:1650	Non-cooled cows	1636:1650	Non-cooled cows	1636:1650	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	9	132	theme	greater	1678:1684	arg1	yield/DMI					1709:1717	=milk yield/DMI	1703:1717	=milk yield/DMI	1703:1717	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	9	132	theme	greater	1678:1684	arg1	efficiency					1691:1700	greater feed efficiency	1678:1700	greater feed efficiency (=milk yield/DMI) at 1 d	1678:1725	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	6	133	with	points	1335:1340	arg1	sample					1362:1367	an additional sample	1348:1367	an additional sample	1348:1367	Rectal temperature and respiration rate were assessed and blood samples were collected at the same time points (with an additional sample at 3 h) for analyses of lactose and cortisol.					
30844051	1	134	theme	source	300:305	arg1	effect					266:271	the effect	262:271	the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion	262:484	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	0	135	dep	fed	33:35	arg1	profile1					207:214	hematologic profile1	195:214	hematologic profile1	195:214	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	0	135	dep	fed	33:35	arg1	intake					155:160	intake	155:160	intake	155:160	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	0	135	dep	fed	33:35	arg1	yield					168:172	milk yield	163:172	milk yield	163:172	Response of lactating dairy cows fed different supplemental zinc sources with and without evaporative cooling to intramammary lipopolysaccharide infusion: intake, milk yield and composition, and hematologic profile1.					
30844051	13	136	theme	greater	2292:2298	arg1	migration					2310:2318	greater leukocyte migration	2292:2318	greater leukocyte migration into the mammary gland	2292:2341	In conclusion, noncooled cows tended to maintain greater feed efficiency and appeared to have greater leukocyte migration into the mammary gland immediately after intramammary LPS infusion compared with cooled cows.					
30844051	1	137	theme	evaporative	311:321	arg1	cooling					323:329	evaporative cooling	311:329	evaporative cooling	311:329	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30844051	2	138	theme	mg/kg	640:644	arg1	sources					609:615	two sources	605:615	two sources of supplemental Zn	605:634	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	138	theme	mg/kg	640:644	arg1	hydroxychloride					649:663	75 mg/kg Zn hydroxychloride	637:663	75 mg/kg Zn hydroxychloride	637:663	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	2	138	theme	mg/kg	640:644	arg1	treatments					551:560	four treatments	546:560	four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC)	546:726	Multiparous Holstein cows (n = 72) were assigned to one of four treatments with a 2×2 factorial arrangement including two sources of supplemental Zn: 75 mg/kg Zn hydroxychloride or 35 mg/kg Zn hydroxychloride + 40 mg/kg Zn-Met complex (ZMC) each with or without evaporative cooling.					
30844051	10	139	theme	apparent	1887:1894	arg1	impact					1896:1901	no apparent impact	1884:1901	no apparent impact	1884:1901	Intramammary LPS infusion dramatically increased (P < 0.01) milk somatic cell count (SCC) but treatments had no apparent impact on milk SCC.					
30844051	9	140	dep	=	1662:1662	arg1	0.09					1664:1667	0.09	1664:1667	0.09	1664:1667	Non-cooled cows tended (P = 0.09) to have greater feed efficiency (=milk yield/DMI) at 1 d after infusion than those subjected to cooling.					
30844051	5	141	theme	Individual	1039:1048	arg1	samples					1055:1061	Individual milk samples	1039:1061	Individual milk samples from both quarters	1039:1080	Individual milk samples from both quarters were collected at -12, -4, 0, 6, 12, 24, 48, 72, 96, 120, 144, and 168 h relative to infusion and analyzed for composition and bovine serum albumin.					
30844051	14	142	contain	had	2445:2447	arg1	source					2438:2443	Dietary supplemental Zn source	2414:2443	Dietary supplemental Zn source	2414:2443	Dietary supplemental Zn source had no impact on measures assessed after intramammary LPS infusion.					
30844051	14	142	contain	had	2445:2447	arg2	impact					2452:2457	no impact	2449:2457	no impact	2449:2457	Dietary supplemental Zn source had no impact on measures assessed after intramammary LPS infusion.					
30844051	1	143	theme	study	239:243	arg1	objective					221:229	The objective	217:229	The objective of this study	217:243	The objective of this study was to determine the effect of dietary supplemental Zn source and evaporative cooling on intake, milk yield and composition, and the rate of leukocyte migration into the mammary gland following intramammary lipopolysaccharide (LPS) infusion.					
30471395	8	0	contain	possessed	951:959	arg2	characteristics					975:989	all desirable characteristics	961:989	all desirable characteristics	961:989	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	8	0	contain	possessed	951:959	arg1	film					946:949	the composite film	932:949	the composite film	932:949	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	7	1	theme	commercial	890:899	arg1	film					914:917	the commercial polyethylene film	886:917	the commercial polyethylene film	886:917	The film containing zinc oxide nanoparticles was effective in reducing the bacterial contamination when compared to the commercial polyethylene film.					
30471395	1	2	theme	zinc	262:265	arg1	oxide					267:271	zinc oxide	262:271	zinc oxide nanoparticles	262:285	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	5	3	theme	ZnO	614:616	arg1	film					632:635	PU/CS/5% nano ZnO biodegradable film	600:635	PU/CS/5% nano ZnO biodegradable film	600:635	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	1	4	theme	oxide	267:271	arg1	nanoparticles					273:285	zinc oxide nanoparticles	262:285	zinc oxide nanoparticles	262:285	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	3	5	theme	%	371:371	arg1	film					392:395	5% nano ZnO composite film	370:395	5% nano ZnO composite film	370:395	PU/CS with 5% nano ZnO composite film showed improved tensile strength and stiffness.					
30471395	5	6	theme	biodegradable	618:630	arg1	film					632:635	PU/CS/5% nano ZnO biodegradable film	600:635	PU/CS/5% nano ZnO biodegradable film	600:635	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	2	7	theme	films	337:341	arg1	properties					306:315	properties	306:315	properties	306:315	The structure and properties of the solvent-cast films were evaluated.					
30471395	2	7	theme	films	337:341	arg1	structure					292:300	structure	292:300	structure	292:300	The structure and properties of the solvent-cast films were evaluated.					
30471395	1	8	theme	nanoparticles	273:285	arg1	proportions					247:257	different proportions	237:257	different proportions of zinc oxide nanoparticles	237:285	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	3	9	theme	nano	373:376	arg1	film					392:395	5% nano ZnO composite film	370:395	5% nano ZnO composite film	370:395	PU/CS with 5% nano ZnO composite film showed improved tensile strength and stiffness.					
30471395	5	10	theme	film	632:635	arg1	%					643:643	86%	641:643	86%	641:643	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	5	10	theme	film	632:635	arg1	loss					592:595	The weight loss	581:595	The weight loss of PU/CS/5% nano ZnO biodegradable film	581:635	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	6	11	theme	pieces	704:709	arg1	period					687:692	The shelf life period	672:692	The shelf life period of carrot pieces wrapped with the composite film	672:741	The shelf life period of carrot pieces wrapped with the composite film was extended up to 9 days.					
30471395	3	12	theme	5	370:370	arg1	%					371:371	%	371:371	%	371:371	PU/CS with 5% nano ZnO composite film showed improved tensile strength and stiffness.					
30471395	1	13	dep	fabricated	150:159	arg1	incorporated					219:230	incorporated	219:230	incorporated with different proportions of zinc oxide nanoparticles	219:285	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	6	14	theme	carrot	697:702	arg1	pieces					704:709	carrot pieces	697:709	carrot pieces wrapped with the composite film	697:741	The shelf life period of carrot pieces wrapped with the composite film was extended up to 9 days.					
30471395	4	15	theme	antibacterial	504:516	arg1	properties					518:527	the antibacterial properties	500:527	the antibacterial properties	500:527	The incorporation of zinc oxide nanoparticles enhanced the antibacterial properties, barrier properties and hydrophobicity of the film.					
30471395	8	16	theme	environmental	1071:1083	arg1	polyurethane					1109:1120	low environmental impact bio-polyol based polyurethane	1067:1120	low environmental impact bio-polyol based polyurethane	1067:1120	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	5	17	theme	PU/CS/5	600:606	arg1	%					607:607	%	607:607	%	607:607	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	1	18	theme	Biodegradable	110:122	arg1	films					139:143	Biodegradable food packaging films	110:143	Biodegradable food packaging films	110:143	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	7	19	theme	bacterial	845:853	arg1	contamination					855:867	the bacterial contamination	841:867	the bacterial contamination	841:867	The film containing zinc oxide nanoparticles was effective in reducing the bacterial contamination when compared to the commercial polyethylene film.					
30471395	3	20	theme	composite	382:390	arg1	film					392:395	5% nano ZnO composite film	370:395	5% nano ZnO composite film	370:395	PU/CS with 5% nano ZnO composite film showed improved tensile strength and stiffness.					
30471395	7	21	theme	oxide	795:799	arg1	nanoparticles					801:813	zinc oxide nanoparticles	790:813	zinc oxide nanoparticles	790:813	The film containing zinc oxide nanoparticles was effective in reducing the bacterial contamination when compared to the commercial polyethylene film.					
30471395	8	22	theme	low	1067:1069	arg1	polyurethane					1109:1120	low environmental impact bio-polyol based polyurethane	1067:1120	low environmental impact bio-polyol based polyurethane	1067:1120	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	5	23	theme	%	607:607	arg1	film					632:635	PU/CS/5% nano ZnO biodegradable film	600:635	PU/CS/5% nano ZnO biodegradable film	600:635	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	1	24	theme	food	124:127	arg1	films					139:143	Biodegradable food packaging films	110:143	Biodegradable food packaging films	110:143	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	8	25	theme	packaging	1018:1026	arg1	material					1028:1035	a food packaging material	1011:1035	a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane	1011:1120	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	3	26	theme	ZnO	378:380	arg1	film					392:395	5% nano ZnO composite film	370:395	5% nano ZnO composite film	370:395	PU/CS with 5% nano ZnO composite film showed improved tensile strength and stiffness.					
30471395	5	27	from	28 days	651:657	arg1	soil					666:669	the soil	662:669	the soil	662:669	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	8	28	theme	bio-polyol	1092:1101	arg1	polyurethane					1109:1120	low environmental impact bio-polyol based polyurethane	1067:1120	low environmental impact bio-polyol based polyurethane	1067:1120	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	5	29	theme	nano	609:612	arg1	film					632:635	PU/CS/5% nano ZnO biodegradable film	600:635	PU/CS/5% nano ZnO biodegradable film	600:635	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	1	30	theme	packaging	129:137	arg1	films					139:143	Biodegradable food packaging films	110:143	Biodegradable food packaging films	110:143	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	6	31	theme	life	682:685	arg1	period					687:692	The shelf life period	672:692	The shelf life period of carrot pieces wrapped with the composite film	672:741	The shelf life period of carrot pieces wrapped with the composite film was extended up to 9 days.					
30471395	2	32	theme	solvent-cast	324:335	arg1	films					337:341	the solvent-cast films	320:341	the solvent-cast films	320:341	The structure and properties of the solvent-cast films were evaluated.					
30471395	8	33	theme	impact	1085:1090	arg1	polyurethane					1109:1120	low environmental impact bio-polyol based polyurethane	1067:1120	low environmental impact bio-polyol based polyurethane	1067:1120	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	4	34	theme	zinc	466:469	arg1	nanoparticles					477:489	zinc oxide nanoparticles	466:489	zinc oxide nanoparticles	466:489	The incorporation of zinc oxide nanoparticles enhanced the antibacterial properties, barrier properties and hydrophobicity of the film.					
30471395	6	35	theme	shelf	676:680	arg1	period					687:692	The shelf life period	672:692	The shelf life period of carrot pieces wrapped with the composite film	672:741	The shelf life period of carrot pieces wrapped with the composite film was extended up to 9 days.					
30471395	2	36	dep	structure	292:300	arg1	The					288:290	The	288:290	The	288:290	The structure and properties of the solvent-cast films were evaluated.					
30471395	7	37	theme	zinc	790:793	arg1	nanoparticles					801:813	zinc oxide nanoparticles	790:813	zinc oxide nanoparticles	790:813	The film containing zinc oxide nanoparticles was effective in reducing the bacterial contamination when compared to the commercial polyethylene film.					
30471395	0	38	theme	composite	47:55	arg1	films					57:61	polyurethane/chitosan/nano ZnO composite films	16:61	polyurethane/chitosan/nano ZnO composite films for biodegradable food packaging applications	16:107	Mahua oil-based polyurethane/chitosan/nano ZnO composite films for biodegradable food packaging applications.					
30471395	8	39	theme	based	1103:1107	arg1	polyurethane					1109:1120	low environmental impact bio-polyol based polyurethane	1067:1120	low environmental impact bio-polyol based polyurethane	1067:1120	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	4	40	theme	nanoparticles	477:489	arg1	incorporation					449:461	The incorporation	445:461	The incorporation of zinc oxide nanoparticles	445:489	The incorporation of zinc oxide nanoparticles enhanced the antibacterial properties, barrier properties and hydrophobicity of the film.					
30471395	0	41	theme	ZnO	43:45	arg1	films					57:61	polyurethane/chitosan/nano ZnO composite films	16:61	polyurethane/chitosan/nano ZnO composite films for biodegradable food packaging applications	16:107	Mahua oil-based polyurethane/chitosan/nano ZnO composite films for biodegradable food packaging applications.					
30471395	7	42	contain	containing	779:788	arg2	nanoparticles					801:813	zinc oxide nanoparticles	790:813	zinc oxide nanoparticles	790:813	The film containing zinc oxide nanoparticles was effective in reducing the bacterial contamination when compared to the commercial polyethylene film.					
30471395	7	42	contain	containing	779:788	arg1	film					774:777	The film	770:777	The film containing zinc oxide nanoparticles	770:813	The film containing zinc oxide nanoparticles was effective in reducing the bacterial contamination when compared to the commercial polyethylene film.					
30471395	7	42	contain	containing	779:788	arg1	effective					819:827	effective	819:827	effective	819:827	The film containing zinc oxide nanoparticles was effective in reducing the bacterial contamination when compared to the commercial polyethylene film.					
30471395	0	43	theme	biodegradable	67:79	arg1	applications					96:107	biodegradable food packaging applications	67:107	biodegradable food packaging applications	67:107	Mahua oil-based polyurethane/chitosan/nano ZnO composite films for biodegradable food packaging applications.					
30471395	4	44	theme	oxide	471:475	arg1	nanoparticles					477:489	zinc oxide nanoparticles	466:489	zinc oxide nanoparticles	466:489	The incorporation of zinc oxide nanoparticles enhanced the antibacterial properties, barrier properties and hydrophobicity of the film.					
30471395	3	45	theme	tensile	413:419	arg1	strength					421:428	improved tensile strength	404:428	improved tensile strength	404:428	PU/CS with 5% nano ZnO composite film showed improved tensile strength and stiffness.					
30471395	4	46	theme	film	575:578	arg1	hydrophobicity					553:566	hydrophobicity	553:566	hydrophobicity	553:566	The incorporation of zinc oxide nanoparticles enhanced the antibacterial properties, barrier properties and hydrophobicity of the film.					
30471395	4	46	theme	film	575:578	arg1	properties					518:527	the antibacterial properties	500:527	the antibacterial properties	500:527	The incorporation of zinc oxide nanoparticles enhanced the antibacterial properties, barrier properties and hydrophobicity of the film.					
30471395	4	46	theme	film	575:578	arg1	properties					538:547	barrier properties	530:547	barrier properties	530:547	The incorporation of zinc oxide nanoparticles enhanced the antibacterial properties, barrier properties and hydrophobicity of the film.					
30471395	8	47	theme	food	1013:1016	arg1	material					1028:1035	a food packaging material	1011:1035	a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane	1011:1120	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	5	48	theme	weight	585:590	arg1	%					643:643	86%	641:643	86%	641:643	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	5	48	theme	weight	585:590	arg1	loss					592:595	The weight loss	581:595	The weight loss of PU/CS/5% nano ZnO biodegradable film	581:635	The weight loss of PU/CS/5% nano ZnO biodegradable film was 86% after 28 days in the soil.					
30471395	3	49	with	PU/CS	359:363	arg1	film					392:395	5% nano ZnO composite film	370:395	5% nano ZnO composite film	370:395	PU/CS with 5% nano ZnO composite film showed improved tensile strength and stiffness.					
30471395	3	50	theme	improved	404:411	arg1	strength					421:428	improved tensile strength	404:428	improved tensile strength	404:428	PU/CS with 5% nano ZnO composite film showed improved tensile strength and stiffness.					
30471395	1	51	theme	Mahua	166:170	arg1	polyurethane					182:193	Mahua oil-based polyurethane	166:193	Mahua oil-based polyurethane (PU)	166:198	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	1	51	theme	Mahua	166:170	arg1	PU					196:197	PU	196:197	PU	196:197	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	7	52	theme	polyethylene	901:912	arg1	film					914:917	the commercial polyethylene film	886:917	the commercial polyethylene film	886:917	The film containing zinc oxide nanoparticles was effective in reducing the bacterial contamination when compared to the commercial polyethylene film.					
30471395	1	53	theme	different	237:245	arg1	proportions					247:257	different proportions	237:257	different proportions of zinc oxide nanoparticles	237:285	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	0	54	theme	packaging	86:94	arg1	applications					96:107	biodegradable food packaging applications	67:107	biodegradable food packaging applications	67:107	Mahua oil-based polyurethane/chitosan/nano ZnO composite films for biodegradable food packaging applications.					
30471395	6	55	theme	composite	728:736	arg1	film					738:741	the composite film	724:741	the composite film	724:741	The shelf life period of carrot pieces wrapped with the composite film was extended up to 9 days.					
30471395	1	56	theme	oil-based	172:180	arg1	polyurethane					182:193	Mahua oil-based polyurethane	166:193	Mahua oil-based polyurethane (PU)	166:198	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	1	56	theme	oil-based	172:180	arg1	PU					196:197	PU	196:197	PU	196:197	Biodegradable food packaging films were fabricated from Mahua oil-based polyurethane (PU) and chitosan (CS), incorporated with different proportions of zinc oxide nanoparticles.					
30471395	0	57	theme	food	81:84	arg1	applications					96:107	biodegradable food packaging applications	67:107	biodegradable food packaging applications	67:107	Mahua oil-based polyurethane/chitosan/nano ZnO composite films for biodegradable food packaging applications.					
30471395	4	58	theme	barrier	530:536	arg1	properties					538:547	barrier properties	530:547	barrier properties	530:547	The incorporation of zinc oxide nanoparticles enhanced the antibacterial properties, barrier properties and hydrophobicity of the film.					
30471395	0	59	theme	polyurethane/chitosan/nano	16:41	arg1	films					57:61	polyurethane/chitosan/nano ZnO composite films	16:61	polyurethane/chitosan/nano ZnO composite films for biodegradable food packaging applications	16:107	Mahua oil-based polyurethane/chitosan/nano ZnO composite films for biodegradable food packaging applications.					
30471395	8	60	theme	desirable	965:973	arg1	characteristics					975:989	all desirable characteristics	961:989	all desirable characteristics	961:989	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
30471395	8	61	theme	composite	936:944	arg1	film					946:949	the composite film	932:949	the composite film	932:949	In essence, the composite film possessed all desirable characteristics to be considered as a food packaging material that has been fabricated from low environmental impact bio-polyol based polyurethane.					
29578019	7	0	theme	0.50	1208:1211	arg1	%					1212:1212	%	1212:1212	%	1212:1212	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	1	1	theme	peel	162:165	arg1	APP					180:182	APP	180:182	APP	180:182	In the present study, apple peel polyphenols (APP) were incorporated into chitosan (CS) to develop a novel functional film.					
29578019	1	1	theme	peel	162:165	arg1	polyphenols					167:177	apple peel polyphenols	156:177	apple peel polyphenols (APP)	156:183	In the present study, apple peel polyphenols (APP) were incorporated into chitosan (CS) to develop a novel functional film.					
29578019	7	2	theme	%	1212:1212	arg1	concentration					1218:1230	0.50% APP concentration	1208:1230	0.50% APP concentration	1208:1230	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	6	3	theme	films	1069:1073	arg1	stability					1043:1051	Thermal stability	1035:1051	Thermal stability of the prepared films	1035:1073	Thermal stability of the prepared films was decreased while antioxidant and antimicrobial activities of the CS-based APP film were significantly increased.					
29578019	1	4	theme	novel	235:239	arg1	film					252:255	a novel functional film	233:255	a novel functional film	233:255	In the present study, apple peel polyphenols (APP) were incorporated into chitosan (CS) to develop a novel functional film.					
29578019	0	5	theme	peel	116:119	arg1	polyphenols					121:131	apple peel polyphenols	110:131	apple peel polyphenols	110:131	Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.					
29578019	7	6	theme	bio-composite	1329:1341	arg1	material					1358:1365	bio-composite food packaging material	1329:1365	bio-composite food packaging material for the food industry	1329:1387	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	7	6	theme	bio-composite	1329:1341	arg1	it					1304:1305	it	1304:1305	it	1304:1305	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	7	7	theme	food	1375:1378	arg1	industry					1380:1387	the food industry	1371:1387	the food industry	1371:1387	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	1	8	theme	functional	241:250	arg1	film					252:255	a novel functional film	233:255	a novel functional film	233:255	In the present study, apple peel polyphenols (APP) were incorporated into chitosan (CS) to develop a novel functional film.					
29578019	0	9	theme	apple	110:114	arg1	polyphenols					121:131	apple peel polyphenols	110:131	apple peel polyphenols	110:131	Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.					
29578019	6	10	theme	CS-based	1143:1150	arg1	film					1156:1159	the CS-based APP film	1139:1159	the CS-based APP film	1139:1159	Thermal stability of the prepared films was decreased while antioxidant and antimicrobial activities of the CS-based APP film were significantly increased.					
29578019	3	11	theme	swelling	556:563	arg1	ration					565:570	swelling ration	556:570	swelling ration	556:570	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	7	12	theme	good	1242:1245	arg1	properties					1276:1285	good mechanical and antimicrobial properties	1242:1285	good mechanical and antimicrobial properties	1242:1285	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	2	13	theme	electron	267:274	arg1	microscopy					276:285	Scanning electron microscopy	258:285	Scanning electron microscopy	258:285	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	5	14	theme	1	937:937	arg1	%					938:938	%	938:938	%	938:938	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	5	15	from	break	907:911	arg1	strength					880:887	Tensile strength	872:887	Tensile strength	872:887	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	5	15	from	break	907:911	arg1	elongation					893:902	elongation	893:902	elongation	893:902	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	2	16	theme	Scanning	258:265	arg1	microscopy					276:285	Scanning electron microscopy	258:285	Scanning electron microscopy	258:285	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	7	17	theme	packaging	1348:1356	arg1	material					1358:1365	bio-composite food packaging material	1329:1365	bio-composite food packaging material for the food industry	1329:1387	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	7	17	theme	packaging	1348:1356	arg1	it					1304:1305	it	1304:1305	it	1304:1305	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	5	18	theme	Tensile	872:878	arg1	strength					880:887	Tensile strength	872:887	Tensile strength	872:887	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	7	19	theme	CS-APP	1191:1196	arg1	film					1198:1201	CS-APP film	1191:1201	CS-APP film with 0.50% APP concentration	1191:1230	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	4	20	theme	opacity	775:781	arg1	ratio					796:800	its thickness, density, solubility, opacity and swelling ratio	739:800	its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased	739:869	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	2	21	theme	potential	398:406	arg1	interaction					408:418	potential interaction	398:418	potential interaction	398:418	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	6	22	theme	APP	1152:1154	arg1	film					1156:1159	the CS-based APP film	1139:1159	the CS-based APP film	1139:1159	Thermal stability of the prepared films was decreased while antioxidant and antimicrobial activities of the CS-based APP film were significantly increased.					
29578019	6	23	theme	prepared	1060:1067	arg1	films					1069:1073	the prepared films	1056:1073	the prepared films	1056:1073	Thermal stability of the prepared films was decreased while antioxidant and antimicrobial activities of the CS-based APP film were significantly increased.					
29578019	7	24	theme	APP	1214:1216	arg1	concentration					1218:1230	0.50% APP concentration	1208:1230	0.50% APP concentration	1208:1230	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	3	25	theme	water	576:580	arg1	permeability					588:599	water vapor permeability	576:599	water vapor permeability	576:599	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	4	26	dep	ratio	796:800	arg1	decreased					861:869	decreased	861:869	were decreased	856:869	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	2	27	theme	films	458:462	arg1	structure					387:395	structure	387:395	structure	387:395	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	2	27	theme	films	458:462	arg1	interaction					408:418	potential interaction	398:418	potential interaction	398:418	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	2	27	theme	films	458:462	arg1	stability					432:440	thermal stability	424:440	thermal stability	424:440	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	5	28	with	film	927:930	arg1	APP					940:942	1% APP	937:942	1% APP	937:942	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	2	29	theme	transform-infrared	296:313	arg1	spectroscopy					315:326	Fourier transform-infrared spectroscopy	288:326	Fourier transform-infrared spectroscopy	288:326	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	5	30	dep	lower	997:1001	arg1	16.48MPa					948:955	16.48MPa	948:955	16.48MPa	948:955	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	5	30	dep	lower	997:1001	arg1	%					966:966	13.33%	961:966	13.33%	961:966	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	2	31	theme	prepared	449:456	arg1	films					458:462	the prepared films	445:462	the prepared films	445:462	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	4	32	theme	thickness	743:751	arg1	ratio					796:800	its thickness, density, solubility, opacity and swelling ratio	739:800	its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased	739:869	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	5	33	theme	CS	1018:1019	arg1	film					1029:1032	CS control film	1018:1032	CS control film	1018:1032	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	2	34	theme	Fourier	288:294	arg1	spectroscopy					315:326	Fourier transform-infrared spectroscopy	288:326	Fourier transform-infrared spectroscopy	288:326	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	6	35	theme	Thermal	1035:1041	arg1	stability					1043:1051	Thermal stability	1035:1051	Thermal stability of the prepared films	1035:1073	Thermal stability of the prepared films was decreased while antioxidant and antimicrobial activities of the CS-based APP film were significantly increased.					
29578019	3	36	theme	vapor	582:586	arg1	permeability					588:599	water vapor permeability	576:599	water vapor permeability	576:599	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	3	37	theme	water	538:542	arg1	solubility					544:553	water solubility	538:553	water solubility	538:553	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	4	38	theme	density	754:760	arg1	ratio					796:800	its thickness, density, solubility, opacity and swelling ratio	739:800	its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased	739:869	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	3	39	theme	moisture	495:502	arg1	content					504:510	moisture content	495:510	moisture content	495:510	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	0	40	theme	antimicrobial	51:63	arg1	film					87:90	chitosan-based antimicrobial active food packaging film	36:90	chitosan-based antimicrobial active food packaging film	36:90	Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.					
29578019	5	41	theme	film	927:930	arg1	strength					880:887	Tensile strength	872:887	Tensile strength	872:887	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	5	41	theme	film	927:930	arg1	elongation					893:902	elongation	893:902	elongation	893:902	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	7	42	with	film	1198:1201	arg1	concentration					1218:1230	0.50% APP concentration	1208:1230	0.50% APP concentration	1208:1230	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	3	43	theme	Physical	465:472	arg1	opacity					529:535	opacity	529:535	opacity	529:535	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	3	43	theme	Physical	465:472	arg1	ration					565:570	swelling ration	556:570	swelling ration	556:570	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	3	43	theme	Physical	465:472	arg1	content					504:510	moisture content	495:510	moisture content	495:510	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	3	43	theme	Physical	465:472	arg1	density					513:519	density	513:519	density	513:519	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	3	43	theme	Physical	465:472	arg1	solubility					544:553	water solubility	538:553	water solubility	538:553	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	3	43	theme	Physical	465:472	arg1	properties					474:483	Physical properties	465:483	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability	465:599	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	3	43	theme	Physical	465:472	arg1	permeability					588:599	water vapor permeability	576:599	water vapor permeability	576:599	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	3	43	theme	Physical	465:472	arg1	color					522:526	color	522:526	color	522:526	Physical properties including moisture content, density, color, opacity, water solubility, swelling ration and water vapor permeability were measured.					
29578019	2	44	theme	thermal	424:430	arg1	stability					432:440	thermal stability	424:440	thermal stability	424:440	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	0	45	theme	chitosan-based	36:49	arg1	film					87:90	chitosan-based antimicrobial active food packaging film	36:90	chitosan-based antimicrobial active food packaging film	36:90	Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.					
29578019	4	46	theme	solubility	763:772	arg1	ratio					796:800	its thickness, density, solubility, opacity and swelling ratio	739:800	its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased	739:869	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	4	47	theme	APP	654:656	arg1	addition					642:649	addition	642:649	addition of APP into CS	642:664	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	0	48	theme	food	72:75	arg1	film					87:90	chitosan-based antimicrobial active food packaging film	36:90	chitosan-based antimicrobial active food packaging film	36:90	Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.					
29578019	4	49	theme	vapor	837:841	arg1	permeability					843:854	water vapor permeability	831:854	water vapor permeability	831:854	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	6	50	theme	antimicrobial	1111:1123	arg1	activities					1125:1134	antioxidant and antimicrobial activities	1095:1134	antioxidant and antimicrobial activities of the CS-based APP film	1095:1159	Thermal stability of the prepared films was decreased while antioxidant and antimicrobial activities of the CS-based APP film were significantly increased.					
29578019	5	51	theme	CS-APP	920:925	arg1	film					927:930	the CS-APP film	916:930	the CS-APP film with 1% APP	916:942	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	0	52	theme	active	65:70	arg1	film					87:90	chitosan-based antimicrobial active food packaging film	36:90	chitosan-based antimicrobial active food packaging film	36:90	Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.					
29578019	5	53	theme	control	1021:1027	arg1	film					1029:1032	CS control film	1018:1032	CS control film	1018:1032	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	4	54	theme	water	831:835	arg1	permeability					843:854	water vapor permeability	831:854	water vapor permeability	831:854	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	4	55	theme	film	720:723	arg1	properties					702:711	the physical properties	689:711	the physical properties of the film	689:723	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	5	56	theme	%	938:938	arg1	APP					940:942	1% APP	937:942	1% APP	937:942	Tensile strength and elongation at break of the CS-APP film with 1% APP was 16.48MPa and 13.33%, respectively, significantly lower than those for CS control film.					
29578019	1	57	theme	present	141:147	arg1	study					149:153	the present study	137:153	the present study	137:153	In the present study, apple peel polyphenols (APP) were incorporated into chitosan (CS) to develop a novel functional film.					
29578019	6	58	theme	antioxidant	1095:1105	arg1	activities					1125:1134	antioxidant and antimicrobial activities	1095:1134	antioxidant and antimicrobial activities of the CS-based APP film	1095:1159	Thermal stability of the prepared films was decreased while antioxidant and antimicrobial activities of the CS-based APP film were significantly increased.					
29578019	6	59	theme	film	1156:1159	arg1	activities					1125:1134	antioxidant and antimicrobial activities	1095:1134	antioxidant and antimicrobial activities of the CS-based APP film	1095:1159	Thermal stability of the prepared films was decreased while antioxidant and antimicrobial activities of the CS-based APP film were significantly increased.					
29578019	4	60	theme	swelling	787:794	arg1	ratio					796:800	its thickness, density, solubility, opacity and swelling ratio	739:800	its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased	739:869	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	4	61	theme	physical	693:700	arg1	properties					702:711	the physical properties	689:711	the physical properties of the film	689:723	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	2	62	theme	thermogravimetric	332:348	arg1	analyses					350:357	thermogravimetric analyses	332:357	thermogravimetric analyses	332:357	Scanning electron microscopy, Fourier transform-infrared spectroscopy and thermogravimetric analyses were performed to study the structure, potential interaction and thermal stability of the prepared films.					
29578019	0	63	theme	film	87:90	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.					
29578019	0	63	theme	film	87:90	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.					
29578019	4	64	theme	moisture	810:817	arg1	content					819:825	moisture content	810:825	moisture content	810:825	The results revealed that addition of APP into CS significantly improved the physical properties of the film by increasing its thickness, density, solubility, opacity and swelling ratio whereas moisture content and water vapor permeability were decreased.					
29578019	7	65	theme	mechanical	1247:1256	arg1	properties					1276:1285	good mechanical and antimicrobial properties	1242:1285	good mechanical and antimicrobial properties	1242:1285	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	7	66	theme	food	1343:1346	arg1	material					1358:1365	bio-composite food packaging material	1329:1365	bio-composite food packaging material for the food industry	1329:1387	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	7	66	theme	food	1343:1346	arg1	it					1304:1305	it	1304:1305	it	1304:1305	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	0	67	theme	packaging	77:85	arg1	film					87:90	chitosan-based antimicrobial active food packaging film	36:90	chitosan-based antimicrobial active food packaging film	36:90	Preparation and characterization of chitosan-based antimicrobial active food packaging film incorporated with apple peel polyphenols.					
29578019	7	68	theme	antimicrobial	1262:1274	arg1	properties					1276:1285	good mechanical and antimicrobial properties	1242:1285	good mechanical and antimicrobial properties	1242:1285	CS-APP film with 0.50% APP concentration exhibited good mechanical and antimicrobial properties, indicating that it could be developed as bio-composite food packaging material for the food industry.					
29578019	1	69	theme	apple	156:160	arg1	APP					180:182	APP	180:182	APP	180:182	In the present study, apple peel polyphenols (APP) were incorporated into chitosan (CS) to develop a novel functional film.					
29578019	1	69	theme	apple	156:160	arg1	polyphenols					167:177	apple peel polyphenols	156:177	apple peel polyphenols (APP)	156:183	In the present study, apple peel polyphenols (APP) were incorporated into chitosan (CS) to develop a novel functional film.					
29716440	1	0	contain	have	129:132	arg2	structure					143:151	a porous structure	134:151	a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue	134:283	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	1	0	contain	have	129:132	arg1	scaffolds					111:119	tissue scaffolds	104:119	tissue scaffolds	104:119	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	2	1	theme	Solidworks	630:639	arg1	Simulation					646:655	Solidworks Flow Simulation	630:655	Solidworks Flow Simulation	630:655	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	3	2	theme	porous	858:863	arg1	materials					829:837	The materials	825:837	The materials printed	825:845	The materials printed are highly porous scaffolds with good mechanical properties.					
29716440	3	2	theme	porous	858:863	arg1	scaffolds					865:873	highly porous scaffolds	851:873	highly porous scaffolds with good mechanical properties	851:905	The materials printed are highly porous scaffolds with good mechanical properties.					
29716440	2	3	theme	batches	493:499	arg1	properties					469:478	rheological properties	457:478	rheological properties of different batches of materials after different crosslinking times	457:547	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	3	theme	batches	493:499	arg1	simulation					550:559	simulation	550:559	simulation of the pneumatic extrusion process	550:594	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	3	theme	batches	493:499	arg1	fabrication					613:623	3D scaffolds fabrication	600:623	3D scaffolds fabrication	600:623	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	4	theme	structure	684:692	arg1	Simulation					646:655	Solidworks Flow Simulation	630:655	Solidworks Flow Simulation	630:655	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	4	theme	structure	684:692	arg1	measurement					702:712	measurement	702:712	measurement of pressure-pull performance	702:741	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	4	theme	structure	684:692	arg1	experiments					748:758	experiments	748:758	experiments aimed at finding out the vitro cytotoxicity and cell morphology	748:822	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	4	theme	structure	684:692	arg1	observation					658:668	observation	658:668	observation of its porous structure by SEM	658:699	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	5	theme	process	343:349	arg1	study					318:322	a study	316:322	a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology	316:822	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	1	6	theme	specific	269:276	arg1	tissue					278:283	the specific tissue	265:283	the specific tissue	265:283	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	2	7	theme	porous	677:682	arg1	structure					684:692	its porous structure	673:692	its porous structure	673:692	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	8	theme	study	318:322	arg1	results					305:311	the results	301:311	the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology	301:822	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	9	theme	scaffolds	603:611	arg1	fabrication					613:623	3D scaffolds fabrication	600:623	3D scaffolds fabrication	600:623	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	10	theme	vitro	785:789	arg1	cytotoxicity					791:802	the vitro cytotoxicity	781:802	the vitro cytotoxicity	781:802	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	11	theme	crosslinking	530:541	arg1	times					543:547	different crosslinking times	520:547	different crosslinking times	520:547	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	12	theme	different	520:528	arg1	times					543:547	different crosslinking times	520:547	different crosslinking times	520:547	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	13	theme	different	483:491	arg1	batches					493:499	different batches	483:499	different batches of materials after different crosslinking times	483:547	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	1	14	theme	tissue	72:77	arg1	applications					91:102	tissue engineering applications	72:102	tissue engineering applications	72:102	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	2	15	theme	oxidized	384:391	arg1	nanocellulose					393:405	nanocellulose	393:405	nanocellulose	393:405	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	0	16	theme	printing	3:10	arg1	process					12:18	3D printing process	0:18	3D printing process of oxidized nanocellulose and gelatin scaffold.	0:66	3D printing process of oxidized nanocellulose and gelatin scaffold.					
29716440	1	17	theme	engineering	79:89	arg1	applications					91:102	tissue engineering applications	72:102	tissue engineering applications	72:102	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	0	18	theme	3D	0:1	arg1	process					12:18	3D printing process	0:18	3D printing process of oxidized nanocellulose and gelatin scaffold.	0:66	3D printing process of oxidized nanocellulose and gelatin scaffold.					
29716440	2	19	theme	Flow	641:644	arg1	Simulation					646:655	Solidworks Flow Simulation	630:655	Solidworks Flow Simulation	630:655	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	1	20	theme	cell	174:177	arg1	proliferation/differentiation					179:207	cell proliferation/differentiation	174:207	cell proliferation/differentiation	174:207	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	2	21	theme	pneumatic	568:576	arg1	process					588:594	the pneumatic extrusion process	564:594	the pneumatic extrusion process	564:594	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	1	22	theme	tissue	104:109	arg1	scaffolds					111:119	tissue scaffolds	104:119	tissue scaffolds	104:119	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	2	23	theme	cell	808:811	arg1	morphology					813:822	cell morphology	808:822	cell morphology	808:822	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	0	24	theme	nanocellulose	32:44	arg1	scaffold					58:65	oxidized nanocellulose and gelatin scaffold	23:65	oxidized nanocellulose and gelatin scaffold	23:65	3D printing process of oxidized nanocellulose and gelatin scaffold.					
29716440	0	25	theme	oxidized	23:30	arg1	scaffold					58:65	oxidized nanocellulose and gelatin scaffold	23:65	oxidized nanocellulose and gelatin scaffold	23:65	3D printing process of oxidized nanocellulose and gelatin scaffold.					
29716440	0	26	theme	gelatin	50:56	arg1	scaffold					58:65	oxidized nanocellulose and gelatin scaffold	23:65	oxidized nanocellulose and gelatin scaffold	23:65	3D printing process of oxidized nanocellulose and gelatin scaffold.					
29716440	2	27	theme	materials	504:512	arg1	batches					493:499	different batches	483:499	different batches of materials after different crosslinking times	483:547	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	1	28	dep	the	161:163	arg1	needs					165:169	needs	165:169	needs	165:169	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	2	29	theme	performance	731:741	arg1	Simulation					646:655	Solidworks Flow Simulation	630:655	Solidworks Flow Simulation	630:655	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	29	theme	performance	731:741	arg1	measurement					702:712	measurement	702:712	measurement of pressure-pull performance	702:741	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	29	theme	performance	731:741	arg1	experiments					748:758	experiments	748:758	experiments aimed at finding out the vitro cytotoxicity and cell morphology	748:822	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	29	theme	performance	731:741	arg1	observation					658:668	observation	658:668	observation of its porous structure by SEM	658:699	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	30	theme	printing	334:341	arg1	process					343:349	the 3D printing process	327:349	the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology	327:822	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	31	theme	rheological	457:467	arg1	properties					469:478	rheological properties	457:478	rheological properties of different batches of materials after different crosslinking times	457:547	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	32	theme	pressure-pull	717:729	arg1	performance					731:741	pressure-pull performance	717:741	pressure-pull performance	717:741	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	33	theme	3D	331:332	arg1	process					343:349	the 3D printing process	327:349	the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology	327:822	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	3	34	theme	mechanical	885:894	arg1	properties					896:905	good mechanical properties	880:905	good mechanical properties	880:905	The materials printed are highly porous scaffolds with good mechanical properties.					
29716440	2	35	theme	process	588:594	arg1	properties					469:478	rheological properties	457:478	rheological properties of different batches of materials after different crosslinking times	457:547	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	35	theme	process	588:594	arg1	simulation					550:559	simulation	550:559	simulation of the pneumatic extrusion process	550:594	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	2	35	theme	process	588:594	arg1	fabrication					613:623	3D scaffolds fabrication	600:623	3D scaffolds fabrication	600:623	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	3	36	with	scaffolds	865:873	arg1	properties					896:905	good mechanical properties	880:905	good mechanical properties	880:905	The materials printed are highly porous scaffolds with good mechanical properties.					
29716440	1	37	theme	sufficient	230:239	arg1	strength					252:259	sufficient mechanical strength	230:259	sufficient mechanical strength	230:259	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	2	38	theme	extrusion	578:586	arg1	process					588:594	the pneumatic extrusion process	564:594	the pneumatic extrusion process	564:594	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29716440	1	39	theme	mechanical	241:250	arg1	strength					252:259	sufficient mechanical strength	230:259	sufficient mechanical strength	230:259	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	1	40	theme	porous	136:141	arg1	structure					143:151	a porous structure	134:151	a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue	134:283	For tissue engineering applications tissue scaffolds need to have a porous structure to meet the needs of cell proliferation/differentiation, vascularisation and sufficient mechanical strength for the specific tissue.					
29716440	3	41	theme	good	880:883	arg1	properties					896:905	good mechanical properties	880:905	good mechanical properties	880:905	The materials printed are highly porous scaffolds with good mechanical properties.					
29716440	0	42	theme	scaffold	58:65	arg1	process					12:18	3D printing process	0:18	3D printing process of oxidized nanocellulose and gelatin scaffold.	0:66	3D printing process of oxidized nanocellulose and gelatin scaffold.					
29716440	2	43	theme	composite	355:363	arg1	materials					365:373	composite materials	355:373	composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology	355:822	Here we report the results of a study of the 3D printing process for composite materials based on oxidized nanocellulose and gelatin, that was optimised through measuring rheological properties of different batches of materials after different crosslinking times, simulation of the pneumatic extrusion process and 3D scaffolds fabrication with Solidworks Flow Simulation, observation of its porous structure by SEM, measurement of pressure-pull performance, and experiments aimed at finding out the vitro cytotoxicity and cell morphology.					
29558455	1	0	theme	different	281:289	arg1	types					299:303	many different surface types	276:303	many different surface types	276:303	Within the Haloferax genus, both the surface (S)-layer protein, and the glycans that can decorate it, vary between species, which can potentially result in many different surface types, analogous to bacterial serotypes.					
29558455	0	1	theme	Protein	74:80	arg1	Glycosylation					82:94	Protein Glycosylation	74:94	Protein Glycosylation in the Genus Haloferax	74:117	Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax.					
29558455	1	2	theme	surface	291:297	arg1	types					299:303	many different surface types	276:303	many different surface types	276:303	Within the Haloferax genus, both the surface (S)-layer protein, and the glycans that can decorate it, vary between species, which can potentially result in many different surface types, analogous to bacterial serotypes.					
29558455	5	3	theme	genetically-related	887:905	arg1	strains					907:913	geographically- and genetically-related strains	867:913	geographically- and genetically-related strains	867:913	Thus, geographically- and genetically-related strains may exhibit diverse surface structures to such an extent that no two isolates present an identical surface profile.					
29558455	3	4	gly	glycoproteins	470:482	arg1	glycoproteins					470:482	S-layer glycoproteins	462:482	S-layer glycoproteins found in multiple Haloferax strains	462:518	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	4	5	theme	major	736:740	arg1	cluster					756:762	the major glycosylation cluster	732:762	the major glycosylation cluster	732:762	We analyze the phylogeny of individual glycosylation genes and demonstrate that while the major glycosylation cluster tends to be conserved among closely related strains, the alternative cluster is highly variable.					
29558455	3	6	theme	Haloferax	502:510	arg1	strains					512:518	multiple Haloferax strains	493:518	multiple Haloferax strains	493:518	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	4	7	theme	genes	699:703	arg1	phylogeny					661:669	the phylogeny	657:669	the phylogeny of individual glycosylation genes	657:703	We analyze the phylogeny of individual glycosylation genes and demonstrate that while the major glycosylation cluster tends to be conserved among closely related strains, the alternative cluster is highly variable.					
29558455	4	8	theme	alternative	821:831	arg1	cluster					833:839	the alternative cluster	817:839	the alternative cluster	817:839	We analyze the phylogeny of individual glycosylation genes and demonstrate that while the major glycosylation cluster tends to be conserved among closely related strains, the alternative cluster is highly variable.					
29558455	4	8	theme	alternative	821:831	arg1	variable					851:858	variable	851:858	variable	851:858	We analyze the phylogeny of individual glycosylation genes and demonstrate that while the major glycosylation cluster tends to be conserved among closely related strains, the alternative cluster is highly variable.					
29558455	0	9	theme	Genus	103:107	arg1	Haloferax					109:117	the Genus Haloferax	99:117	the Genus Haloferax	99:117	Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax.					
29558455	0	10	theme	Glycoproteins	38:50	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Surface Layer Glycoproteins and Genes	0:60	Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax.					
29558455	1	11	theme	bacterial	319:327	arg1	serotypes					329:337	bacterial serotypes	319:337	bacterial serotypes	319:337	Within the Haloferax genus, both the surface (S)-layer protein, and the glycans that can decorate it, vary between species, which can potentially result in many different surface types, analogous to bacterial serotypes.					
29558455	5	12	theme	surface	935:941	arg1	structures					943:952	diverse surface structures	927:952	diverse surface structures	927:952	Thus, geographically- and genetically-related strains may exhibit diverse surface structures to such an extent that no two isolates present an identical surface profile.					
29558455	3	13	from	clusters	601:608	arg1	sites					639:643	two coastal sites	627:643	two coastal sites	627:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	0	14	theme	Comparative	0:10	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Surface Layer Glycoproteins and Genes	0:60	Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax.					
29558455	3	15	theme	comparative	532:542	arg1	analyses					553:560	comparative genomics analyses	532:560	comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites	532:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	4	16	theme	glycosylation	742:754	arg1	cluster					756:762	the major glycosylation cluster	732:762	the major glycosylation cluster	732:762	We analyze the phylogeny of individual glycosylation genes and demonstrate that while the major glycosylation cluster tends to be conserved among closely related strains, the alternative cluster is highly variable.					
29558455	0	17	theme	Surface	24:30	arg1	Glycoproteins					38:50	Surface Layer Glycoproteins	24:50	Surface Layer Glycoproteins	24:50	Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax.					
29558455	5	18	theme	diverse	927:933	arg1	structures					943:952	diverse surface structures	927:952	diverse surface structures	927:952	Thus, geographically- and genetically-related strains may exhibit diverse surface structures to such an extent that no two isolates present an identical surface profile.					
29558455	5	19	theme	identical	1004:1012	arg1	profile					1022:1028	an identical surface profile	1001:1028	an identical surface profile	1001:1028	Thus, geographically- and genetically-related strains may exhibit diverse surface structures to such an extent that no two isolates present an identical surface profile.					
29558455	1	20	theme	-layer	168:173	arg1	protein					175:181	both the surface (S)-layer protein	148:181	protein	175:181	Within the Haloferax genus, both the surface (S)-layer protein, and the glycans that can decorate it, vary between species, which can potentially result in many different surface types, analogous to bacterial serotypes.					
29558455	0	21	from	Glycosylation	82:94	arg1	Haloferax					109:117	the Genus Haloferax	99:117	the Genus Haloferax	99:117	Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax.					
29558455	2	22	theme	mating	424:429	arg1	preferences					431:441	mating preferences	424:441	mating preferences	424:441	This variation may mediate phenotypes, such as sensitivity to different viruses and mating preferences.					
29558455	4	23	theme	related	800:806	arg1	strains					808:814	closely related strains	792:814	closely related strains	792:814	We analyze the phylogeny of individual glycosylation genes and demonstrate that while the major glycosylation cluster tends to be conserved among closely related strains, the alternative cluster is highly variable.					
29558455	3	24	located	found	484:488	arg2	glycoproteins					470:482	S-layer glycoproteins	462:482	S-layer glycoproteins found in multiple Haloferax strains	462:518	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	3	24	located	found	484:488	arg1	strains					512:518	multiple Haloferax strains	493:518	multiple Haloferax strains	493:518	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	0	25	theme	Layer	32:36	arg1	Glycoproteins					38:50	Surface Layer Glycoproteins	24:50	Surface Layer Glycoproteins	24:50	Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax.					
29558455	3	26	theme	major	565:569	arg1	clusters					601:608	major and alternative glycosylation clusters	565:608	major and alternative glycosylation clusters of isolates from two coastal sites	565:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	0	27	theme	Genes	56:60	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Surface Layer Glycoproteins and Genes	0:60	Comparative Analysis of Surface Layer Glycoproteins and Genes Involved in Protein Glycosylation in the Genus Haloferax.					
29558455	3	28	theme	isolates	613:620	arg1	clusters					601:608	major and alternative glycosylation clusters	565:608	major and alternative glycosylation clusters of isolates from two coastal sites	565:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	3	29	theme	genomics	544:551	arg1	analyses					553:560	comparative genomics analyses	532:560	comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites	532:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	4	30	theme	glycosylation	685:697	arg1	genes					699:703	individual glycosylation genes	674:703	individual glycosylation genes	674:703	We analyze the phylogeny of individual glycosylation genes and demonstrate that while the major glycosylation cluster tends to be conserved among closely related strains, the alternative cluster is highly variable.					
29558455	2	31	theme	different	402:410	arg1	viruses					412:418	different viruses	402:418	different viruses	402:418	This variation may mediate phenotypes, such as sensitivity to different viruses and mating preferences.					
29558455	4	32	theme	individual	674:683	arg1	genes					699:703	individual glycosylation genes	674:703	individual glycosylation genes	674:703	We analyze the phylogeny of individual glycosylation genes and demonstrate that while the major glycosylation cluster tends to be conserved among closely related strains, the alternative cluster is highly variable.					
29558455	3	33	theme	alternative	575:585	arg1	clusters					601:608	major and alternative glycosylation clusters	565:608	major and alternative glycosylation clusters of isolates from two coastal sites	565:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	3	34	theme	glycosylation	587:599	arg1	clusters					601:608	major and alternative glycosylation clusters	565:608	major and alternative glycosylation clusters of isolates from two coastal sites	565:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	5	35	theme	surface	1014:1020	arg1	profile					1022:1028	an identical surface profile	1001:1028	an identical surface profile	1001:1028	Thus, geographically- and genetically-related strains may exhibit diverse surface structures to such an extent that no two isolates present an identical surface profile.					
29558455	3	36	theme	coastal	631:637	arg1	sites					639:643	two coastal sites	627:643	two coastal sites	627:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	3	37	theme	clusters	601:608	arg1	analyses					553:560	comparative genomics analyses	532:560	comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites	532:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	1	38	theme	Haloferax	131:139	arg1	genus					141:145	the Haloferax genus	127:145	the Haloferax genus	127:145	Within the Haloferax genus, both the surface (S)-layer protein, and the glycans that can decorate it, vary between species, which can potentially result in many different surface types, analogous to bacterial serotypes.					
29558455	3	39	theme	multiple	493:500	arg1	strains					512:518	multiple Haloferax strains	493:518	multiple Haloferax strains	493:518	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	3	40	theme	S-layer	462:468	arg1	glycoproteins					470:482	S-layer glycoproteins	462:482	S-layer glycoproteins found in multiple Haloferax strains	462:518	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	3	41	from	sites	639:643	arg1	isolates					613:620	isolates	613:620	isolates from two coastal sites	613:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	3	41	from	sites	639:643	arg1	clusters					601:608	major and alternative glycosylation clusters	565:608	major and alternative glycosylation clusters of isolates from two coastal sites	565:643	Here, we describe S-layer glycoproteins found in multiple Haloferax strains and perform comparative genomics analyses of major and alternative glycosylation clusters of isolates from two coastal sites.					
29558455	1	42	theme	many	276:279	arg1	types					299:303	many different surface types	276:303	many different surface types	276:303	Within the Haloferax genus, both the surface (S)-layer protein, and the glycans that can decorate it, vary between species, which can potentially result in many different surface types, analogous to bacterial serotypes.					
29558455	1	43	dep	protein	175:181	arg1	both					148:151	both	148:151	both	148:151	Within the Haloferax genus, both the surface (S)-layer protein, and the glycans that can decorate it, vary between species, which can potentially result in many different surface types, analogous to bacterial serotypes.					
29558455	5	44	theme	geographically-	867:881	arg1	strains					907:913	geographically- and genetically-related strains	867:913	geographically- and genetically-related strains	867:913	Thus, geographically- and genetically-related strains may exhibit diverse surface structures to such an extent that no two isolates present an identical surface profile.					
35021376	0	0	theme	Glycoprotein	65:76	arg1	Strategy					89:96	A Selective Glycoprotein Microarray Strategy	53:96	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.	0:97	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.					
35021376	3	1	theme	patterns	326:333	arg1	monitoring					305:314	direct monitoring	298:314	direct monitoring of glycan patterns of glycoproteins	298:350	Therefore, direct monitoring of glycan patterns of glycoproteins is closely related to cancer progression as well as metastasis.					
35021376	1	2	theme	complicated	140:150	arg1	modifications					187:199	the most complicated but significant post-translational modifications	131:199	the most complicated but significant post-translational modifications	131:199	Protein glycosylation is one of the most complicated but significant post-translational modifications.					
35021376	6	3	theme	glycoproteins	980:992	arg1	patterns					968:975	specific lectin-recognition patterns	940:975	specific lectin-recognition patterns	940:975	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	4	4	theme	high-abundant	582:594	arg1	proteins					612:619	high-abundant nonglycosylated proteins	582:619	high-abundant nonglycosylated proteins	582:619	In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.					
35021376	0	5	theme	Selective	55:63	arg1	Strategy					89:96	A Selective Glycoprotein Microarray Strategy	53:96	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.	0:97	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.					
35021376	4	6	theme	glass	518:522	arg1	slides					524:529	glass slides	518:529	glass slides	518:529	In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.					
35021376	5	7	theme	undesired	678:686	arg1	absorption					700:709	the undesired nonspecific absorption	674:709	the undesired nonspecific absorption	674:709	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	6	8	from	sera	1126:1129	arg1	patterns					1106:1113	glycan patterns	1099:1113	glycan patterns from human sera without tedious sample preparation	1099:1164	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	8	from	sera	1126:1129	arg1	monitoring					1085:1094	direct monitoring	1078:1094	direct monitoring of glycan patterns from human sera without tedious sample preparation	1078:1164	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	9	theme	sample	1147:1152	arg1	preparation					1154:1164	tedious sample preparation	1139:1164	tedious sample preparation	1139:1164	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	2	10	from	alterations	208:218	arg1	structure					230:238	glycan structure	223:238	glycan structure	223:238	Minor alterations in glycan structure can considerably affect the biology of a cell.					
35021376	5	11	theme	nonspecific	688:698	arg1	absorption					700:709	the undesired nonspecific absorption	674:709	the undesired nonspecific absorption	674:709	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	4	12	theme	proteins	612:619	arg1	presence					570:577	the presence	566:577	the presence of high-abundant nonglycosylated proteins	566:619	In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.					
35021376	0	13	theme	Microarray	78:87	arg1	Strategy					89:96	A Selective Glycoprotein Microarray Strategy	53:96	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.	0:97	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.					
35021376	0	14	from	Sera	47:50	arg1	Screening					7:15	Direct Screening	0:15	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.	0:97	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.					
35021376	8	15	theme	cancer	1402:1407	arg1	screening					1409:1417	cancer screening	1402:1417	cancer screening	1402:1417	Remarkably, the distinguishable glycan patterns of the three groups make them a powerful platform for cancer screening and prediagnosis.					
35021376	4	16	theme	nonglycosylated	596:610	arg1	proteins					612:619	high-abundant nonglycosylated proteins	582:619	high-abundant nonglycosylated proteins	582:619	In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.					
35021376	5	17	theme	chemistry	779:787	arg1	conditions					810:819	the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions	712:819	the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions	712:819	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	4	18	theme	-tosyl-directed	450:464	arg1	strategy					466:473	a boronic acid (BA)-tosyl-directed strategy	431:473	a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides	431:529	In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.					
35021376	5	19	theme	immobilization	637:650	arg1	efficiency					652:661	the immobilization efficiency	633:661	the immobilization efficiency	633:661	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	6	20	theme	tedious	1139:1145	arg1	preparation					1154:1164	tedious sample preparation	1139:1164	tedious sample preparation	1139:1164	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	4	21	gly	glycoproteins	501:513	arg1	glycoproteins					501:513	glycoproteins	501:513	glycoproteins	501:513	In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.					
35021376	1	22	theme	significant	156:166	arg1	modifications					187:199	the most complicated but significant post-translational modifications	131:199	the most complicated but significant post-translational modifications	131:199	Protein glycosylation is one of the most complicated but significant post-translational modifications.					
35021376	2	23	theme	cell	281:284	arg1	biology					268:274	the biology	264:274	the biology of a cell	264:284	Minor alterations in glycan structure can considerably affect the biology of a cell.					
35021376	1	24	theme	post-translational	168:185	arg1	modifications					187:199	the most complicated but significant post-translational modifications	131:199	the most complicated but significant post-translational modifications	131:199	Protein glycosylation is one of the most complicated but significant post-translational modifications.					
35021376	7	25	theme	pancreatic	1236:1245	arg1	cancer					1247:1252	pancreatic cancer	1236:1252	pancreatic cancer	1236:1252	Three serum groups comprised of healthy controls and lung cancer and pancreatic cancer patients were analyzed by this new technique.					
35021376	6	26	theme	lectin-recognition	949:966	arg1	patterns					968:975	specific lectin-recognition patterns	940:975	specific lectin-recognition patterns	940:975	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	7	27	theme	new	1285:1287	arg1	technique					1289:1297	this new technique	1280:1297	this new technique	1280:1297	Three serum groups comprised of healthy controls and lung cancer and pancreatic cancer patients were analyzed by this new technique.					
35021376	5	28	theme	surface	793:799	arg1	blocking					801:808	surface blocking	793:808	surface blocking	793:808	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	6	29	theme	interest	997:1004	arg1	glycoproteins					980:992	glycoproteins	980:992	glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS)	980:1051	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	3	30	theme	glycan	319:324	arg1	patterns					326:333	glycan patterns	319:333	glycan patterns of glycoproteins	319:350	Therefore, direct monitoring of glycan patterns of glycoproteins is closely related to cancer progression as well as metastasis.					
35021376	1	31	theme	modifications	187:199	arg1	one					124:126	one	124:126	one	124:126	Protein glycosylation is one of the most complicated but significant post-translational modifications.					
35021376	1	31	theme	modifications	187:199	arg1	modifications					187:199	the most complicated but significant post-translational modifications	131:199	the most complicated but significant post-translational modifications	131:199	Protein glycosylation is one of the most complicated but significant post-translational modifications.					
35021376	0	32	theme	Direct	0:5	arg1	Screening					7:15	Direct Screening	0:15	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.	0:97	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.					
35021376	7	33	theme	lung	1220:1223	arg1	cancer					1225:1230	lung cancer	1220:1230	lung cancer	1220:1230	Three serum groups comprised of healthy controls and lung cancer and pancreatic cancer patients were analyzed by this new technique.					
35021376	4	34	gly	nonglycosylated	596:610	arg1	proteins					612:619	high-abundant nonglycosylated proteins	582:619	high-abundant nonglycosylated proteins	582:619	In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.					
35021376	6	35	theme	glycoprotein	897:908	arg1	platform					921:928	The optimized glycoprotein microarray platform	883:928	The optimized glycoprotein microarray platform	883:928	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	8	36	theme	glycan	1332:1337	arg1	patterns					1339:1346	the distinguishable glycan patterns	1312:1346	the distinguishable glycan patterns of the three groups	1312:1366	Remarkably, the distinguishable glycan patterns of the three groups make them a powerful platform for cancer screening and prediagnosis.					
35021376	6	37	theme	human	1120:1124	arg1	sera					1126:1129	human sera	1120:1129	human sera without tedious sample preparation	1120:1164	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	0	38	theme	Glycan	20:25	arg1	Patterns					27:34	Glycan Patterns	20:34	Glycan Patterns	20:34	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.					
35021376	5	39	theme	azide	739:743	arg1	chemistry					779:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	5	40	theme	data	877:880	arg1	collection					854:863	the collection	850:863	the collection of reliable data	850:880	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	5	41	theme	cycloaddition	745:757	arg1	chemistry					779:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	6	42	theme	specific	940:947	arg1	patterns					968:975	specific lectin-recognition patterns	940:975	specific lectin-recognition patterns	940:975	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	1	43	theme	Protein	99:105	arg1	glycosylation					107:119	Protein glycosylation	99:119	Protein glycosylation	99:119	Protein glycosylation is one of the most complicated but significant post-translational modifications.					
35021376	6	44	theme	patterns	1106:1113	arg1	monitoring					1085:1094	direct monitoring	1078:1094	direct monitoring of glycan patterns from human sera without tedious sample preparation	1078:1164	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	45	gly	glycoprotein	897:908	arg1	glycoprotein					897:908	The optimized glycoprotein microarray platform	883:928	The optimized glycoprotein microarray platform	883:928	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	7	46	theme	cancer	1247:1252	arg1	patients					1254:1261	lung cancer and pancreatic cancer patients	1220:1261	lung cancer and pancreatic cancer patients	1220:1261	Three serum groups comprised of healthy controls and lung cancer and pancreatic cancer patients were analyzed by this new technique.					
35021376	6	47	theme	bovine	1034:1039	arg1	serum					1041:1045	fetal bovine serum	1028:1045	fetal bovine serum (FBS)	1028:1051	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	47	theme	bovine	1034:1039	arg1	FBS					1048:1050	FBS	1048:1050	FBS	1048:1050	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	48	theme	glycan	1099:1104	arg1	patterns					1106:1113	glycan patterns	1099:1113	glycan patterns from human sera without tedious sample preparation	1099:1164	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	0	49	theme	Patterns	27:34	arg1	Screening					7:15	Direct Screening	0:15	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.	0:97	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.					
35021376	6	50	theme	fetal	1028:1032	arg1	serum					1041:1045	fetal bovine serum	1028:1045	fetal bovine serum (FBS)	1028:1051	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	50	theme	fetal	1028:1032	arg1	FBS					1048:1050	FBS	1048:1050	FBS	1048:1050	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	5	51	theme	SPAAC	760:764	arg1	chemistry					779:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	5	52	theme	conjugation	767:777	arg1	chemistry					779:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	5	53	theme	reliable	868:875	arg1	data					877:880	reliable data	868:880	reliable data	868:880	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	7	54	theme	cancer	1225:1230	arg1	patients					1254:1261	lung cancer and pancreatic cancer patients	1220:1261	lung cancer and pancreatic cancer patients	1220:1261	Three serum groups comprised of healthy controls and lung cancer and pancreatic cancer patients were analyzed by this new technique.					
35021376	7	55	theme	healthy	1199:1205	arg1	controls					1207:1214	healthy controls	1199:1214	healthy controls	1199:1214	Three serum groups comprised of healthy controls and lung cancer and pancreatic cancer patients were analyzed by this new technique.					
35021376	6	56	from	patterns	968:975	arg1	serum					1041:1045	fetal bovine serum	1028:1045	fetal bovine serum (FBS)	1028:1051	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	56	from	patterns	968:975	arg1	lysate					1017:1022	E. coil lysate	1009:1022	E. coil lysate	1009:1022	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	56	from	patterns	968:975	arg1	FBS					1048:1050	FBS	1048:1050	FBS	1048:1050	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	57	from	lysate	1017:1022	arg1	patterns					968:975	specific lectin-recognition patterns	940:975	specific lectin-recognition patterns	940:975	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	58	theme	microarray	910:919	arg1	platform					921:928	The optimized glycoprotein microarray platform	883:928	The optimized glycoprotein microarray platform	883:928	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	5	59	theme	strain-promoted	716:730	arg1	chemistry					779:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	8	60	theme	groups	1361:1366	arg1	patterns					1339:1346	the distinguishable glycan patterns	1312:1346	the distinguishable glycan patterns of the three groups	1312:1366	Remarkably, the distinguishable glycan patterns of the three groups make them a powerful platform for cancer screening and prediagnosis.					
35021376	0	61	theme	Human	41:45	arg1	Sera					47:50	Human Sera	41:50	Human Sera	41:50	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.					
35021376	3	62	gly	glycoproteins	338:350	arg1	glycoproteins					338:350	glycoproteins	338:350	glycoproteins	338:350	Therefore, direct monitoring of glycan patterns of glycoproteins is closely related to cancer progression as well as metastasis.					
35021376	2	63	theme	glycan	223:228	arg1	structure					230:238	glycan structure	223:238	glycan structure	223:238	Minor alterations in glycan structure can considerably affect the biology of a cell.					
35021376	6	64	from	monitoring	1085:1094	arg1	sera					1126:1129	human sera	1120:1129	human sera without tedious sample preparation	1120:1164	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	65	theme	coil	1012:1015	arg1	lysate					1017:1022	E. coil lysate	1009:1022	E. coil lysate	1009:1022	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	3	66	theme	direct	298:303	arg1	monitoring					305:314	direct monitoring	298:314	direct monitoring of glycan patterns of glycoproteins	298:350	Therefore, direct monitoring of glycan patterns of glycoproteins is closely related to cancer progression as well as metastasis.					
35021376	6	67	theme	optimized	887:895	arg1	platform					921:928	The optimized glycoprotein microarray platform	883:928	The optimized glycoprotein microarray platform	883:928	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	68	from	glycoproteins	980:992	arg1	serum					1041:1045	fetal bovine serum	1028:1045	fetal bovine serum (FBS)	1028:1051	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	68	from	glycoproteins	980:992	arg1	lysate					1017:1022	E. coil lysate	1009:1022	E. coil lysate	1009:1022	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	6	68	from	glycoproteins	980:992	arg1	FBS					1048:1050	FBS	1048:1050	FBS	1048:1050	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	8	69	theme	distinguishable	1316:1330	arg1	patterns					1339:1346	the distinguishable glycan patterns	1312:1346	the distinguishable glycan patterns of the three groups	1312:1366	Remarkably, the distinguishable glycan patterns of the three groups make them a powerful platform for cancer screening and prediagnosis.					
35021376	6	70	gly	glycoproteins	980:992	arg1	glycoproteins					980:992	glycoproteins	980:992	glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS)	980:1051	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	5	71	theme	alkyne	732:737	arg1	chemistry					779:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry	716:787	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	4	72	theme	boronic	433:439	arg1	strategy					466:473	a boronic acid (BA)-tosyl-directed strategy	431:473	a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides	431:529	In this study, a boronic acid (BA)-tosyl-directed strategy to selectively immobilize glycoproteins on glass slides was successfully developed even in the presence of high-abundant nonglycosylated proteins.					
35021376	7	73	theme	serum	1173:1177	arg1	groups					1179:1184	Three serum groups	1167:1184	Three serum groups comprised of healthy controls and lung cancer and pancreatic cancer patients	1167:1261	Three serum groups comprised of healthy controls and lung cancer and pancreatic cancer patients were analyzed by this new technique.					
35021376	6	74	theme	direct	1078:1083	arg1	monitoring					1085:1094	direct monitoring	1078:1094	direct monitoring of glycan patterns from human sera without tedious sample preparation	1078:1164	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	3	75	theme	glycoproteins	338:350	arg1	patterns					326:333	glycan patterns	319:333	glycan patterns of glycoproteins	319:350	Therefore, direct monitoring of glycan patterns of glycoproteins is closely related to cancer progression as well as metastasis.					
35021376	2	76	theme	Minor	202:206	arg1	alterations					208:218	Minor alterations	202:218	Minor alterations in glycan structure	202:238	Minor alterations in glycan structure can considerably affect the biology of a cell.					
35021376	0	77	dep	Screening	7:15	arg1	Strategy					89:96	A Selective Glycoprotein Microarray Strategy	53:96	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.	0:97	Direct Screening of Glycan Patterns from Human Sera: A Selective Glycoprotein Microarray Strategy.					
35021376	8	78	theme	powerful	1380:1387	arg1	platform					1389:1396	a powerful platform	1378:1396	a powerful platform for cancer screening and prediagnosis	1378:1434	Remarkably, the distinguishable glycan patterns of the three groups make them a powerful platform for cancer screening and prediagnosis.					
35021376	6	79	from	serum	1041:1045	arg1	patterns					968:975	specific lectin-recognition patterns	940:975	specific lectin-recognition patterns	940:975	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	5	80	theme	blocking	801:808	arg1	conditions					810:819	the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions	712:819	the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions	712:819	To enhance the immobilization efficiency and reduce the undesired nonspecific absorption, the strain-promoted alkyne azide cycloaddition (SPAAC) conjugation chemistry and surface blocking conditions were carefully optimized for the collection of reliable data.					
35021376	6	81	theme	E.	1009:1010	arg1	lysate					1017:1022	E. coil lysate	1009:1022	E. coil lysate	1009:1022	The optimized glycoprotein microarray platform describes specific lectin-recognition patterns of glycoproteins of interest in E. coil lysate and fetal bovine serum (FBS), which encourages us for direct monitoring of glycan patterns from human sera without tedious sample preparation.					
35021376	3	82	theme	cancer	374:379	arg1	progression					381:391	cancer progression	374:391	cancer progression as well as metastasis	374:413	Therefore, direct monitoring of glycan patterns of glycoproteins is closely related to cancer progression as well as metastasis.					
31374274	5	0	theme	20°C	700:703	arg1	dosage					725:730	20°C, pH=7 and adsorbent dosage	700:730	20°C, pH=7 and adsorbent dosage	700:730	The results showed that the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage is 0.01g.					
31374274	5	1	from	dosage	725:730	arg1	202mgg-1					688:695	the adsorption capacities of GC hydrogels for Co2+ 202mgg-1	637:695	the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage	637:730	The results showed that the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage is 0.01g.					
31374274	3	2	from	influence	440:448	arg1	process					484:490	the adsorption process	469:490	the adsorption process	469:490	In the study of adsorption process, the influence of five factors on the adsorption process was studied.					
31374274	5	3	theme	GC	666:667	arg1	hydrogels					669:677	GC hydrogels	666:677	GC hydrogels	666:677	The results showed that the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage is 0.01g.					
31374274	6	4	theme	adsorbent	813:821	arg1	hydrogel					804:811	GC hydrogel adsorbent	801:821	GC hydrogel adsorbent	801:821	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	3	5	theme	adsorption	473:482	arg1	process					484:490	the adsorption process	469:490	the adsorption process	469:490	In the study of adsorption process, the influence of five factors on the adsorption process was studied.					
31374274	5	6	theme	hydrogels	669:677	arg1	capacities					652:661	the adsorption capacities	637:661	the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage	637:730	The results showed that the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage is 0.01g.					
31374274	6	7	theme	GC	801:802	arg1	hydrogel					804:811	GC hydrogel adsorbent	801:821	GC hydrogel adsorbent	801:821	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	4	8	theme	isotherm	588:595	arg1	mechanism					575:583	adsorption mechanism	564:583	adsorption mechanism	564:583	On this basis, the adsorption thermodynamics, kinetics and adsorption mechanism of isotherm were discussed.					
31374274	4	8	theme	isotherm	588:595	arg1	thermodynamics					535:548	the adsorption thermodynamics	520:548	the adsorption thermodynamics	520:548	On this basis, the adsorption thermodynamics, kinetics and adsorption mechanism of isotherm were discussed.					
31374274	4	8	theme	isotherm	588:595	arg1	kinetics					551:558	kinetics	551:558	kinetics	551:558	On this basis, the adsorption thermodynamics, kinetics and adsorption mechanism of isotherm were discussed.					
31374274	6	9	theme	ions	793:796	arg1	chemisorption					844:856	a single molecule chemisorption	826:856	a single molecule chemisorption of the spontaneous process	826:883	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	6	9	theme	ions	793:796	arg1	adsorption					767:776	the adsorption	763:776	the adsorption of heavy metal ions by GC hydrogel adsorbent	763:821	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	2	10	dep	transform	286:294	arg1	infrared					296:303	infrared	296:303	transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA)	286:397	The samples were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA).					
31374274	2	11	theme	electron	336:343	arg1	SEM					357:359	SEM	357:359	SEM	357:359	The samples were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA).					
31374274	2	11	theme	electron	336:343	arg1	microscopy					345:354	scanning electron microscopy	327:354	scanning electron microscopy (SEM)	327:360	The samples were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA).					
31374274	0	12	theme	chitosan-glucose	26:41	arg1	hydrogel					43:50	composite chitosan-glucose hydrogel	16:50	composite chitosan-glucose hydrogel	16:50	Construction of composite chitosan-glucose hydrogel for adsorption of Co2+ ions.					
31374274	1	13	from	Co2+	224:227	arg1	wastewater					232:241	wastewater	232:241	wastewater	232:241	Glucose and chitosan are compounded on a hydrogel (GC hydrogel) by an initiator and a crosslinking agent, and GC hydrogel are applied to treat Co2+ in wastewater.					
31374274	4	14	theme	adsorption	564:573	arg1	mechanism					575:583	adsorption mechanism	564:583	adsorption mechanism	564:583	On this basis, the adsorption thermodynamics, kinetics and adsorption mechanism of isotherm were discussed.					
31374274	4	14	theme	adsorption	564:573	arg1	thermodynamics					535:548	the adsorption thermodynamics	520:548	the adsorption thermodynamics	520:548	On this basis, the adsorption thermodynamics, kinetics and adsorption mechanism of isotherm were discussed.					
31374274	0	15	theme	composite	16:24	arg1	hydrogel					43:50	composite chitosan-glucose hydrogel	16:50	composite chitosan-glucose hydrogel	16:50	Construction of composite chitosan-glucose hydrogel for adsorption of Co2+ ions.					
31374274	6	16	theme	process	877:883	arg1	chemisorption					844:856	a single molecule chemisorption	826:856	a single molecule chemisorption of the spontaneous process	826:883	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	6	16	theme	process	877:883	arg1	adsorption					767:776	the adsorption	763:776	the adsorption of heavy metal ions by GC hydrogel adsorbent	763:821	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	5	17	theme	adsorption	641:650	arg1	capacities					652:661	the adsorption capacities	637:661	the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage	637:730	The results showed that the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage is 0.01g.					
31374274	6	18	theme	metal	787:791	arg1	ions					793:796	heavy metal ions	781:796	heavy metal ions	781:796	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	2	19	theme	thermogravimetric	366:382	arg1	TGA					394:396	TGA	394:396	TGA	394:396	The samples were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA).					
31374274	2	19	theme	thermogravimetric	366:382	arg1	analysis					384:391	thermogravimetric analysis	366:391	thermogravimetric analysis (TGA)	366:397	The samples were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA).					
31374274	5	20	theme	pH=7	706:709	arg1	dosage					725:730	20°C, pH=7 and adsorbent dosage	700:730	20°C, pH=7 and adsorbent dosage	700:730	The results showed that the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage is 0.01g.					
31374274	6	21	theme	spontaneous	865:875	arg1	process					877:883	the spontaneous process	861:883	the spontaneous process	861:883	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	5	22	theme	capacities	652:661	arg1	202mgg-1					688:695	the adsorption capacities of GC hydrogels for Co2+ 202mgg-1	637:695	the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage	637:730	The results showed that the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage is 0.01g.					
31374274	6	23	theme	heavy	781:785	arg1	ions					793:796	heavy metal ions	781:796	heavy metal ions	781:796	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	0	24	theme	hydrogel	43:50	arg1	Construction					0:11	Construction	0:11	Construction of composite chitosan-glucose hydrogel for adsorption of Co2+ ions.	0:79	Construction of composite chitosan-glucose hydrogel for adsorption of Co2+ ions.					
31374274	7	25	theme	good	925:928	arg1	stability					937:945	good cyclic stability	925:945	good cyclic stability	925:945	In addition, GC hydrogel adsorbent has good cyclic stability.					
31374274	5	26	theme	adsorbent	715:723	arg1	dosage					725:730	20°C, pH=7 and adsorbent dosage	700:730	20°C, pH=7 and adsorbent dosage	700:730	The results showed that the adsorption capacities of GC hydrogels for Co2+ 202mgg-1 at 20°C, pH=7 and adsorbent dosage is 0.01g.					
31374274	7	27	contain	has	921:923	arg2	stability					937:945	good cyclic stability	925:945	good cyclic stability	925:945	In addition, GC hydrogel adsorbent has good cyclic stability.					
31374274	7	27	contain	has	921:923	arg1	hydrogel					902:909	GC hydrogel adsorbent	899:919	GC hydrogel adsorbent	899:919	In addition, GC hydrogel adsorbent has good cyclic stability.					
31374274	7	28	theme	cyclic	930:935	arg1	stability					937:945	good cyclic stability	925:945	good cyclic stability	925:945	In addition, GC hydrogel adsorbent has good cyclic stability.					
31374274	3	29	theme	adsorption	416:425	arg1	process					427:433	adsorption process	416:433	adsorption process	416:433	In the study of adsorption process, the influence of five factors on the adsorption process was studied.					
31374274	7	30	theme	adsorbent	911:919	arg1	hydrogel					902:909	GC hydrogel adsorbent	899:919	GC hydrogel adsorbent	899:919	In addition, GC hydrogel adsorbent has good cyclic stability.					
31374274	6	31	theme	molecule	835:842	arg1	chemisorption					844:856	a single molecule chemisorption	826:856	a single molecule chemisorption of the spontaneous process	826:883	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	6	31	theme	molecule	835:842	arg1	adsorption					767:776	the adsorption	763:776	the adsorption of heavy metal ions by GC hydrogel adsorbent	763:821	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	1	32	theme	GC	132:133	arg1	hydrogel					122:129	a hydrogel	120:129	a hydrogel (GC hydrogel)	120:143	Glucose and chitosan are compounded on a hydrogel (GC hydrogel) by an initiator and a crosslinking agent, and GC hydrogel are applied to treat Co2+ in wastewater.					
31374274	1	32	theme	GC	132:133	arg1	hydrogel					135:142	GC hydrogel	132:142	GC hydrogel	132:142	Glucose and chitosan are compounded on a hydrogel (GC hydrogel) by an initiator and a crosslinking agent, and GC hydrogel are applied to treat Co2+ in wastewater.					
31374274	2	33	dep	Fourier	278:284	arg1	transform					286:294	transform	286:294	transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA)	286:397	The samples were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA).					
31374274	1	34	theme	GC	191:192	arg1	hydrogel					194:201	GC hydrogel	191:201	GC hydrogel	191:201	Glucose and chitosan are compounded on a hydrogel (GC hydrogel) by an initiator and a crosslinking agent, and GC hydrogel are applied to treat Co2+ in wastewater.					
31374274	7	35	theme	GC	899:900	arg1	hydrogel					902:909	GC hydrogel adsorbent	899:919	GC hydrogel adsorbent	899:919	In addition, GC hydrogel adsorbent has good cyclic stability.					
31374274	3	36	theme	process	427:433	arg1	study					407:411	the study	403:411	the study of adsorption process	403:433	In the study of adsorption process, the influence of five factors on the adsorption process was studied.					
31374274	4	37	theme	adsorption	524:533	arg1	mechanism					575:583	adsorption mechanism	564:583	adsorption mechanism	564:583	On this basis, the adsorption thermodynamics, kinetics and adsorption mechanism of isotherm were discussed.					
31374274	4	37	theme	adsorption	524:533	arg1	thermodynamics					535:548	the adsorption thermodynamics	520:548	the adsorption thermodynamics	520:548	On this basis, the adsorption thermodynamics, kinetics and adsorption mechanism of isotherm were discussed.					
31374274	4	37	theme	adsorption	524:533	arg1	kinetics					551:558	kinetics	551:558	kinetics	551:558	On this basis, the adsorption thermodynamics, kinetics and adsorption mechanism of isotherm were discussed.					
31374274	0	38	theme	ions	75:78	arg1	adsorption					56:65	adsorption	56:65	adsorption of Co2+ ions	56:78	Construction of composite chitosan-glucose hydrogel for adsorption of Co2+ ions.					
31374274	1	39	theme	crosslinking	167:178	arg1	agent					180:184	a crosslinking agent	165:184	a crosslinking agent	165:184	Glucose and chitosan are compounded on a hydrogel (GC hydrogel) by an initiator and a crosslinking agent, and GC hydrogel are applied to treat Co2+ in wastewater.					
31374274	2	40	theme	scanning	327:334	arg1	SEM					357:359	SEM	357:359	SEM	357:359	The samples were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA).					
31374274	2	40	theme	scanning	327:334	arg1	microscopy					345:354	scanning electron microscopy	327:354	scanning electron microscopy (SEM)	327:360	The samples were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA).					
31374274	0	41	theme	Co2+	70:73	arg1	ions					75:78	Co2+ ions	70:78	Co2+ ions	70:78	Construction of composite chitosan-glucose hydrogel for adsorption of Co2+ ions.					
31374274	6	42	theme	single	828:833	arg1	chemisorption					844:856	a single molecule chemisorption	826:856	a single molecule chemisorption of the spontaneous process	826:883	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	6	42	theme	single	828:833	arg1	adsorption					767:776	the adsorption	763:776	the adsorption of heavy metal ions by GC hydrogel adsorbent	763:821	The study shows that the adsorption of heavy metal ions by GC hydrogel adsorbent is a single molecule chemisorption of the spontaneous process.					
31374274	3	43	theme	factors	458:464	arg1	influence					440:448	the influence	436:448	the influence of five factors on the adsorption process	436:490	In the study of adsorption process, the influence of five factors on the adsorption process was studied.					
31669466	6	0	theme	broad	1107:1111	arg1	range					1116:1120	broad pH range	1107:1120	broad pH range	1107:1120	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	9	1	from	removal	1525:1531	arg1	treatment					1562:1570	drinking water treatment	1547:1570	drinking water treatment	1547:1570	This work suggests 2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment.					
31669466	6	2	theme	5 min	1153:1157	arg1	time					1168:1171	5 min reaction time	1153:1171	5 min reaction time	1153:1171	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	9	3	theme	Ag-TiO2/Al2O3/CS	1480:1495	arg1	nano-composite					1497:1510	2D Ag-TiO2/Al2O3/CS nano-composite	1477:1510	2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment	1477:1570	This work suggests 2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment.					
31669466	2	4	from	solutions	480:488	arg1	irradiation					455:465	UVA irradiation	451:465	UVA irradiation from aqueous solutions	451:488	In this study, ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite was synthesized for the fast reduction of nitrate under UVA irradiation from aqueous solutions.					
31669466	9	5	theme	nitrate	1536:1542	arg1	removal					1525:1531	the fast removal	1516:1531	the fast removal of nitrate in drinking water treatment	1516:1570	This work suggests 2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment.					
31669466	4	6	theme	contact	804:810	arg1	time					812:815	contact time	804:815	contact time	804:815	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	1	7	theme	nitrogen	261:268	arg1	cycle					270:274	the nitrogen cycle	257:274	the nitrogen cycle	257:274	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	7	8	theme	Ag-doped	1216:1223	arg1	heterostructures					1236:1251	The Ag-doped and hybrid heterostructures	1212:1251	heterostructures	1236:1251	The Ag-doped and hybrid heterostructures can effectively utilize UV-visible-light to remove nitrate and degrade formic acid.					
31669466	6	9	from	removal	1133:1139	arg1	time					1168:1171	5 min reaction time	1153:1171	5 min reaction time	1153:1171	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	3	10	theme	N2	600:601	arg1	isotherms					625:633	N2 adsorption-desorption isotherms	600:633	N2 adsorption-desorption isotherms	600:633	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	2	11	theme	ultra-thin	311:320	arg1	nano-composite					380:393	ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite	311:393	ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite	311:393	In this study, ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite was synthesized for the fast reduction of nitrate under UVA irradiation from aqueous solutions.					
31669466	6	12	theme	fast	1081:1084	arg1	reduction					1094:1102	fast nitrate reduction	1081:1102	fast nitrate reduction	1081:1102	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	0	13	theme	nitrate	122:128	arg1	reduction					109:117	enhanced photocatalytic reduction	85:117	enhanced photocatalytic reduction of nitrate	85:128	Fabrication of novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite photocatalyst toward enhanced photocatalytic reduction of nitrate.					
31669466	8	14	theme	third	1411:1415	arg1	cycle					1417:1421	the third cycle	1407:1421	the third cycle	1407:1421	For the 3 cycles the photocatalyst efficiency remained same and after the third cycle, its efficacy decreased gradually.					
31669466	7	15	theme	formic	1324:1329	arg1	acid					1331:1334	formic acid	1324:1334	formic acid	1324:1334	The Ag-doped and hybrid heterostructures can effectively utilize UV-visible-light to remove nitrate and degrade formic acid.					
31669466	3	16	theme	adsorption-desorption	603:623	arg1	isotherms					625:633	N2 adsorption-desorption isotherms	600:633	N2 adsorption-desorption isotherms	600:633	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	6	17	theme	water	1185:1189	arg1	conditions					1200:1209	diverse water chemical conditions	1177:1209	diverse water chemical conditions	1177:1209	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	6	18	theme	74	1129:1130	arg1	%					1131:1131	%	1131:1131	%	1131:1131	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	4	19	theme	reduction	884:892	arg1	rate					868:871	the rate	864:871	the rate of nitrate reduction	864:892	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	4	20	theme	photocatalyst	781:793	arg1	dose					795:798	photocatalyst dose	781:798	photocatalyst dose	781:798	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	6	21	theme	diverse	1177:1183	arg1	conditions					1200:1209	diverse water chemical conditions	1177:1209	diverse water chemical conditions	1177:1209	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	4	22	theme	nitrate	876:882	arg1	reduction					884:892	nitrate reduction	876:892	nitrate reduction	876:892	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	2	23	theme	fast	419:422	arg1	reduction					424:432	the fast reduction	415:432	the fast reduction of nitrate	415:443	In this study, ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite was synthesized for the fast reduction of nitrate under UVA irradiation from aqueous solutions.					
31669466	7	24	theme	hybrid	1229:1234	arg1	heterostructures					1236:1251	The Ag-doped and hybrid heterostructures	1212:1251	heterostructures	1236:1251	The Ag-doped and hybrid heterostructures can effectively utilize UV-visible-light to remove nitrate and degrade formic acid.					
31669466	9	25	from	nitrate	1536:1542	arg1	treatment					1562:1570	drinking water treatment	1547:1570	drinking water treatment	1547:1570	This work suggests 2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment.					
31669466	1	26	dep	it	184:185	arg1	use					187:189	use	187:189	use as a drinking water supply	187:216	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	1	27	theme	drinking	196:203	arg1	supply					211:216	a drinking water supply	194:216	a drinking water supply	194:216	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	2	28	theme	aqueous	472:478	arg1	solutions					480:488	aqueous solutions	472:488	aqueous solutions	472:488	In this study, ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite was synthesized for the fast reduction of nitrate under UVA irradiation from aqueous solutions.					
31669466	1	29	theme	aquatic	279:285	arg1	systems					287:293	aquatic systems	279:293	aquatic systems	279:293	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	4	30	from	effect	854:859	arg1	rate					868:871	the rate	864:871	the rate of nitrate reduction	864:892	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	3	31	theme	infrared	654:661	arg1	spectroscopy					663:674	Fourier-transform infrared spectroscopy	636:674	Fourier-transform infrared spectroscopy	636:674	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	1	32	theme	water	205:209	arg1	supply					211:216	a drinking water supply	194:216	a drinking water supply	194:216	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	0	33	theme	2D	21:22	arg1	nano-composite					49:62	novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite	15:62	novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite	15:62	Fabrication of novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite photocatalyst toward enhanced photocatalytic reduction of nitrate.					
31669466	6	34	theme	synthesized	1044:1054	arg1	nano-composite					1056:1069	the synthesized nano-composite	1040:1069	the synthesized nano-composite	1040:1069	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	6	35	from	pH	1144:1145	arg1	removal					1133:1139	74% removal	1129:1139	74% removal at pH 11 in 5 min reaction time	1129:1171	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	5	36	dep	acid	979:982	arg1	nitrate					984:990	nitrate	984:990	formic acid:nitrate	972:990	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	2	37	theme	UVA	451:453	arg1	irradiation					455:465	UVA irradiation	451:465	UVA irradiation from aqueous solutions	451:488	In this study, ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite was synthesized for the fast reduction of nitrate under UVA irradiation from aqueous solutions.					
31669466	0	38	theme	novel	15:19	arg1	nano-composite					49:62	novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite	15:62	novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite	15:62	Fabrication of novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite photocatalyst toward enhanced photocatalytic reduction of nitrate.					
31669466	9	39	from	treatment	1562:1570	arg1	removal					1525:1531	the fast removal	1516:1531	the fast removal of nitrate in drinking water treatment	1516:1570	This work suggests 2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment.					
31669466	6	40	theme	chemical	1191:1198	arg1	conditions					1200:1209	diverse water chemical conditions	1177:1209	diverse water chemical conditions	1177:1209	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	5	41	theme	2:1	967:969	arg1	concentration					950:962	optimal concentration	942:962	optimal concentration of 2:1 (formic acid:nitrate)	942:991	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	0	42	theme	nano-composite	49:62	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite	0:62	Fabrication of novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite photocatalyst toward enhanced photocatalytic reduction of nitrate.					
31669466	3	43	theme	X-ray	547:551	arg1	diffraction					553:563	X-ray diffraction	547:563	X-ray diffraction	547:563	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	5	44	theme	optimal	942:948	arg1	concentration					950:962	optimal concentration	942:962	optimal concentration of 2:1 (formic acid:nitrate)	942:991	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	3	45	theme	UV-vis	680:685	arg1	spectroscopy					707:718	UV-vis diffuse reflectance spectroscopy	680:718	UV-vis diffuse reflectance spectroscopy	680:718	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	5	46	theme	formic	972:977	arg1	acid					979:982	formic acid	972:982	formic acid:nitrate	972:990	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	2	47	theme	nitrate	437:443	arg1	reduction					424:432	the fast reduction	415:432	the fast reduction of nitrate	415:443	In this study, ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite was synthesized for the fast reduction of nitrate under UVA irradiation from aqueous solutions.					
31669466	0	48	theme	Ag-TiO2/γ-Al2O3/Chitosan	24:47	arg1	nano-composite					49:62	novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite	15:62	novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite	15:62	Fabrication of novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite photocatalyst toward enhanced photocatalytic reduction of nitrate.					
31669466	5	49	theme	Formic	895:900	arg1	acid					902:905	Formic acid	895:905	Formic acid	895:905	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	5	49	theme	Formic	895:900	arg1	scavenger					929:937	a radical scavenger	919:937	a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate)	919:991	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	6	50	theme	nitrate	1086:1092	arg1	reduction					1094:1102	fast nitrate reduction	1081:1102	fast nitrate reduction	1081:1102	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	3	51	theme	diffuse	687:693	arg1	spectroscopy					707:718	UV-vis diffuse reflectance spectroscopy	680:718	UV-vis diffuse reflectance spectroscopy	680:718	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	3	52	theme	As-synthesized	491:504	arg1	nano-composite					506:519	As-synthesized nano-composite	491:519	As-synthesized nano-composite	491:519	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	8	53	theme	photocatalyst	1358:1370	arg1	efficiency					1372:1381	the photocatalyst efficiency	1354:1381	the photocatalyst efficiency	1354:1381	For the 3 cycles the photocatalyst efficiency remained same and after the third cycle, its efficacy decreased gradually.					
31669466	6	54	theme	UVA	1023:1025	arg1	irradiation					1027:1037	UVA irradiation	1023:1037	UVA irradiation	1023:1037	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	9	55	theme	water	1556:1560	arg1	treatment					1562:1570	drinking water treatment	1547:1570	drinking water treatment	1547:1570	This work suggests 2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment.					
31669466	5	56	from	concentration	950:962	arg1	acid					902:905	Formic acid	895:905	Formic acid	895:905	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	5	56	from	concentration	950:962	arg1	scavenger					929:937	a radical scavenger	919:937	a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate)	919:991	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	2	57	theme	Ag-TiO2/Al2O3/CS	362:377	arg1	nano-composite					380:393	ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite	311:393	ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite	311:393	In this study, ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite was synthesized for the fast reduction of nitrate under UVA irradiation from aqueous solutions.					
31669466	1	58	attach	presence	135:142	arg1	groundwater					165:175	groundwater	165:175	groundwater	165:175	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	1	58	attach	presence	135:142	arg2	nitrate					154:160	excess nitrate	147:160	excess nitrate	147:160	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	1	59	from	balance	249:255	arg1	systems					287:293	aquatic systems	279:293	aquatic systems	279:293	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	9	60	theme	fast	1520:1523	arg1	removal					1525:1531	the fast removal	1516:1531	the fast removal of nitrate in drinking water treatment	1516:1570	This work suggests 2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment.					
31669466	9	61	theme	drinking	1547:1554	arg1	water					1556:1560	drinking water	1547:1560	drinking water treatment	1547:1570	This work suggests 2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment.					
31669466	1	62	theme	excess	147:152	arg1	nitrate					154:160	excess nitrate	147:160	excess nitrate	147:160	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	3	63	theme	Fourier-transform	636:652	arg1	spectroscopy					663:674	Fourier-transform infrared spectroscopy	636:674	Fourier-transform infrared spectroscopy	636:674	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	7	64	dep	utilize	1269:1275	arg1	UV-visible-light					1277:1292	UV-visible-light	1277:1292	can effectively utilize UV-visible-light to remove nitrate and degrade formic acid	1253:1334	The Ag-doped and hybrid heterostructures can effectively utilize UV-visible-light to remove nitrate and degrade formic acid.					
31669466	6	65	theme	pH	1113:1114	arg1	range					1116:1120	broad pH range	1107:1120	broad pH range	1107:1120	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	1	66	theme	nitrate	154:160	arg1	presence					135:142	The presence	131:142	The presence of excess nitrate in groundwater	131:175	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	1	67	from	presence	135:142	arg1	groundwater					165:175	groundwater	165:175	groundwater	165:175	The presence of excess nitrate in groundwater limits it use as a drinking water supply and its removal is critical to balance the nitrogen cycle in aquatic systems.					
31669466	5	68	used	used	911:914	arg2	scavenger					929:937	a radical scavenger	919:937	a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate)	919:991	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	5	68	used	used	911:914	arg2	acid					902:905	Formic acid	895:905	Formic acid	895:905	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	5	69	dep	concentration	950:962	arg1	acid					979:982	formic acid	972:982	formic acid:nitrate	972:990	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	2	70	theme	2-dimensional	322:334	arg1	nano-composite					380:393	ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite	311:393	ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite	311:393	In this study, ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite was synthesized for the fast reduction of nitrate under UVA irradiation from aqueous solutions.					
31669466	2	71	theme	Ag-TiO2/γ-Al2O3/Chitosan	336:359	arg1	nano-composite					380:393	ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite	311:393	ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite	311:393	In this study, ultra-thin 2-dimensional Ag-TiO2/γ-Al2O3/Chitosan (Ag-TiO2/Al2O3/CS) nano-composite was synthesized for the fast reduction of nitrate under UVA irradiation from aqueous solutions.					
31669466	0	72	theme	photocatalytic	94:107	arg1	reduction					109:117	enhanced photocatalytic reduction	85:117	enhanced photocatalytic reduction of nitrate	85:128	Fabrication of novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite photocatalyst toward enhanced photocatalytic reduction of nitrate.					
31669466	6	73	theme	reaction	1159:1166	arg1	time					1168:1171	5 min reaction time	1153:1171	5 min reaction time	1153:1171	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	3	74	theme	reflectance	695:705	arg1	spectroscopy					707:718	UV-vis diffuse reflectance spectroscopy	680:718	UV-vis diffuse reflectance spectroscopy	680:718	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	6	75	theme	%	1131:1131	arg1	removal					1133:1139	74% removal	1129:1139	74% removal at pH 11 in 5 min reaction time	1129:1171	The results showed that upon UVA irradiation, the synthesized nano-composite exhibited fast nitrate reduction in broad pH range (about 74% removal at pH 11 in 5 min reaction time) in diverse water chemical conditions.					
31669466	3	76	theme	transmission	566:577	arg1	microscopy					588:597	transmission electron microscopy	566:597	transmission electron microscopy	566:597	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	4	77	theme	nitrate	758:764	arg1	concentration					766:778	nitrate concentration	758:778	nitrate concentration	758:778	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	4	78	theme	Experimental	721:732	arg1	pH					754:755	pH	754:755	pH	754:755	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	4	78	theme	Experimental	721:732	arg1	concentration					766:778	nitrate concentration	758:778	nitrate concentration	758:778	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	4	78	theme	Experimental	721:732	arg1	variables					734:742	Experimental variables	721:742	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time	721:815	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	4	78	theme	Experimental	721:732	arg1	time					812:815	contact time	804:815	contact time	804:815	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	4	78	theme	Experimental	721:732	arg1	dose					795:798	photocatalyst dose	781:798	photocatalyst dose	781:798	Experimental variables including pH, nitrate concentration, photocatalyst dose and contact time were considered to demonstrate their effect on the rate of nitrate reduction.					
31669466	0	79	theme	enhanced	85:92	arg1	reduction					109:117	enhanced photocatalytic reduction	85:117	enhanced photocatalytic reduction of nitrate	85:128	Fabrication of novel 2D Ag-TiO2/γ-Al2O3/Chitosan nano-composite photocatalyst toward enhanced photocatalytic reduction of nitrate.					
31669466	5	80	theme	radical	921:927	arg1	acid					902:905	Formic acid	895:905	Formic acid	895:905	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	5	80	theme	radical	921:927	arg1	scavenger					929:937	a radical scavenger	919:937	a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate)	919:991	Formic acid was used as a radical scavenger at optimal concentration of 2:1 (formic acid:nitrate).					
31669466	3	81	theme	electron	579:586	arg1	microscopy					588:597	transmission electron microscopy	566:597	transmission electron microscopy	566:597	As-synthesized nano-composite was well characterized by X-ray diffraction, transmission electron microscopy, N2 adsorption-desorption isotherms, Fourier-transform infrared spectroscopy and UV-vis diffuse reflectance spectroscopy.					
31669466	9	82	theme	2D	1477:1478	arg1	nano-composite					1497:1510	2D Ag-TiO2/Al2O3/CS nano-composite	1477:1510	2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment	1477:1570	This work suggests 2D Ag-TiO2/Al2O3/CS nano-composite for the fast removal of nitrate in drinking water treatment.					
30500502	6	0	theme	physical	1018:1025	arg1	characteristics					1027:1041	The physical characteristics	1014:1041	The physical characteristics of spray-dried powders	1014:1064	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	2	1	theme	anthocyanin	356:366	arg1	material					387:394	a core material	380:394	a core material for microencapsulation	380:417	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	2	1	theme	anthocyanin	356:366	arg1	extract					368:374	anthocyanin extract	356:374	anthocyanin extract	356:374	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	0	2	theme	rice	79:82	arg1	starch					84:89	modified rice starch	70:89	modified rice starch	70:89	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	6	3	theme	ratio	1223:1227	arg1	term					1087:1090	term	1087:1090	term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020)	1087:1235	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	5	4	theme	process	895:901	arg1	parameters					903:912	the spray drying process parameters	878:912	the spray drying process parameters	878:912	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	5	5	theme	parameters	903:912	arg1	values					868:873	The optimum values	856:873	The optimum values of the spray drying process parameters	856:912	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	5	5	theme	parameters	903:912	arg1	%					923:923	6.01%	919:923	6.01% of starch concentration	919:947	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	5	5	theme	parameters	903:912	arg1	concentration					935:947	starch concentration	928:947	starch concentration	928:947	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	4	6	theme	parameters	577:586	arg1	content					738:744	anthocyanin content	726:744	anthocyanin content	726:744	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	6	theme	parameters	577:586	arg1	effect					555:560	The effect	551:560	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency	551:723	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	6	theme	parameters	577:586	arg1	activity					795:802	2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity	747:802	2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity	747:802	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	6	theme	parameters	577:586	arg1	density					810:816	true density	805:816	true density	805:816	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	6	theme	parameters	577:586	arg1	activity					828:835	water activity	822:835	water activity	822:835	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	5	7	theme	atomizer	995:1002	arg1	pressure					1004:1011	4.96 MPa atomizer pressure	986:1011	4.96 MPa atomizer pressure	986:1011	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	6	8	theme	Hausner	1215:1221	arg1	1.020					1230:1234	1.020	1230:1234	1.020	1230:1234	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	6	8	theme	Hausner	1215:1221	arg1	ratio					1223:1227	the Hausner ratio	1211:1227	the Hausner ratio (1.020)	1211:1235	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	6	9	theme	density	1144:1150	arg1	term					1087:1090	term	1087:1090	term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020)	1087:1235	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	2	10	theme	modified	293:300	arg1	material					341:348	wall material	336:348	wall material where anthocyanin extract was a core material for microencapsulation	336:417	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	2	10	theme	modified	293:300	arg1	starch					317:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	1	11	theme	stable	262:267	arg1	powder					281:286	stable anthocyanin powder	262:286	stable anthocyanin powder	262:286	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	5	12	theme	starch	928:933	arg1	concentration					935:947	starch concentration	928:947	starch concentration	928:947	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	0	13	from	bran	59:62	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.	0:128	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	5	14	theme	air	966:968	arg1	temperature					970:980	inlet air temperature	960:980	inlet air temperature	960:980	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	4	15	theme	2,2-diphenyl-1-picrylhydrazyl	747:775	arg1	activity					795:802	2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity	747:802	2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity	747:802	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	1	16	theme	anthocyanin	269:279	arg1	powder					281:286	stable anthocyanin powder	262:286	stable anthocyanin powder	262:286	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	4	17	from	effect	555:560	arg1	parameters					680:689	the dependent parameters	666:689	the dependent parameters	666:689	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	17	from	effect	555:560	arg1	efficiency					714:723	encapsulation efficiency	700:723	encapsulation efficiency	700:723	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	18	theme	air	623:625	arg1	parameters					577:586	independent parameters	565:586	independent parameters	565:586	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	18	theme	air	623:625	arg1	temperature					627:637	inlet air temperature	617:637	inlet air temperature	617:637	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	3	19	theme	central	525:531	arg1	design					543:548	a rotatable central composite design	513:548	a rotatable central composite design	513:548	The microencapsulation was carried out in a spray dryer, and the process was optimized using a rotatable central composite design.					
30500502	7	20	theme	thermal	1242:1248	arg1	crystallinity					1251:1263	crystallinity	1251:1263	crystallinity	1251:1263	The thermal, crystallinity and surface morphology of the microencapsulated particles were also comprehensively studied using DSC, XRD, FTIR, and SEM.					
30500502	5	21	theme	optimum	860:866	arg1	values					868:873	The optimum values	856:873	The optimum values of the spray drying process parameters	856:912	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	5	21	theme	optimum	860:866	arg1	%					923:923	6.01%	919:923	6.01% of starch concentration	919:947	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	5	21	theme	optimum	860:866	arg1	concentration					935:947	starch concentration	928:947	starch concentration	928:947	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	4	22	from	activity	828:835	arg1	parameters					680:689	the dependent parameters	666:689	the dependent parameters	666:689	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	22	from	activity	828:835	arg1	efficiency					714:723	encapsulation efficiency	700:723	encapsulation efficiency	700:723	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	2	23	used	used	328:331	arg2	starch					317:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	2	23	used	used	328:331	arg2	material					341:348	wall material	336:348	wall material where anthocyanin extract was a core material for microencapsulation	336:417	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	3	24	theme	composite	533:541	arg1	design					543:548	a rotatable central composite design	513:548	a rotatable central composite design	513:548	The microencapsulation was carried out in a spray dryer, and the process was optimized using a rotatable central composite design.					
30500502	6	25	theme	activity	1101:1108	arg1	term					1087:1090	term	1087:1090	term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020)	1087:1235	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	1	26	theme	anthocyanin	173:183	arg1	extract					185:191	anthocyanin extract	173:191	anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder	173:286	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	6	27	theme	bulk	1139:1142	arg1	1.404 g/cm3					1153:1163	1.404 g/cm3	1153:1163	1.404 g/cm3	1153:1163	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	6	27	theme	bulk	1139:1142	arg1	6.44 μm					1198:1204	6.44 μm	1198:1204	6.44 μm	1198:1204	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	6	27	theme	bulk	1139:1142	arg1	density					1144:1150	bulk density	1139:1150	bulk density (1.404 g/cm3)	1139:1164	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	4	28	from	content	738:744	arg1	parameters					680:689	the dependent parameters	666:689	the dependent parameters	666:689	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	28	from	content	738:744	arg1	efficiency					714:723	encapsulation efficiency	700:723	encapsulation efficiency	700:723	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	29	theme	anthocyanin	726:736	arg1	content					738:744	anthocyanin content	726:744	anthocyanin content	726:744	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	2	30	theme	core	382:385	arg1	material					387:394	a core material	380:394	a core material for microencapsulation	380:417	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	2	30	theme	core	382:385	arg1	extract					368:374	anthocyanin extract	356:374	anthocyanin extract	356:374	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	5	31	theme	concentration	935:947	arg1	values					868:873	The optimum values	856:873	The optimum values of the spray drying process parameters	856:912	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	5	31	theme	concentration	935:947	arg1	%					923:923	6.01%	919:923	6.01% of starch concentration	919:947	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	5	31	theme	concentration	935:947	arg1	concentration					935:947	starch concentration	928:947	starch concentration	928:947	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	0	32	theme	extract	34:40	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.	0:128	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	7	33	theme	surface	1269:1275	arg1	morphology					1277:1286	surface morphology	1269:1286	surface morphology	1269:1286	The thermal, crystallinity and surface morphology of the microencapsulated particles were also comprehensively studied using DSC, XRD, FTIR, and SEM.					
30500502	0	34	theme	dough	114:118	arg1	rheology					120:127	rice dough rheology	109:127	rice dough rheology	109:127	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	7	35	theme	particles	1313:1321	arg1	crystallinity					1251:1263	crystallinity	1251:1263	crystallinity	1251:1263	The thermal, crystallinity and surface morphology of the microencapsulated particles were also comprehensively studied using DSC, XRD, FTIR, and SEM.					
30500502	7	35	theme	particles	1313:1321	arg1	morphology					1277:1286	surface morphology	1269:1286	surface morphology	1269:1286	The thermal, crystallinity and surface morphology of the microencapsulated particles were also comprehensively studied using DSC, XRD, FTIR, and SEM.					
30500502	2	36	theme	rice	312:315	arg1	material					341:348	wall material	336:348	wall material where anthocyanin extract was a core material for microencapsulation	336:417	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	2	36	theme	rice	312:315	arg1	starch					317:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	9	37	from	effect	1545:1550	arg1	rheology					1572:1579	the steady-shear rheology	1555:1579	the steady-shear rheology of the rice dough	1555:1597	The microencapsulated particles also have an effect on the steady-shear rheology of the rice dough.					
30500502	1	38	theme	purple	198:203	arg1	bran					210:213	purple rice bran	198:213	purple rice bran with modified glutinous rice starch	198:249	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	0	39	theme	anthocyanin	22:32	arg1	extract					34:40	anthocyanin extract	22:40	anthocyanin extract	22:40	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	4	40	theme	independent	565:575	arg1	viz.					589:592	viz.	589:592	viz.	589:592	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	40	theme	independent	565:575	arg1	pressure					653:660	atomizer pressure	644:660	atomizer pressure	644:660	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	40	theme	independent	565:575	arg1	parameters					577:586	independent parameters	565:586	independent parameters	565:586	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	40	theme	independent	565:575	arg1	temperature					627:637	inlet air temperature	617:637	inlet air temperature	617:637	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	40	theme	independent	565:575	arg1	concentration					602:614	starch concentration	595:614	starch concentration	595:614	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	0	41	theme	rice	109:112	arg1	rheology					120:127	rice dough rheology	109:127	rice dough rheology	109:127	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	0	42	from	starch	84:89	arg1	rheology					120:127	rice dough rheology	109:127	rice dough rheology	109:127	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	2	43	theme	glutinous	302:310	arg1	material					341:348	wall material	336:348	wall material where anthocyanin extract was a core material for microencapsulation	336:417	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	2	43	theme	glutinous	302:310	arg1	starch					317:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	1	44	theme	rice	205:208	arg1	bran					210:213	purple rice bran	198:213	purple rice bran with modified glutinous rice starch	198:249	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	0	45	theme	purple	47:52	arg1	bran					59:62	purple rice bran	47:62	purple rice bran using modified rice starch and its effect on rice dough rheology	47:127	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	5	46	theme	inlet	960:964	arg1	temperature					970:980	inlet air temperature	960:980	inlet air temperature	960:980	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	6	47	theme	powders	1058:1064	arg1	characteristics					1027:1041	The physical characteristics	1014:1041	The physical characteristics of spray-dried powders	1014:1064	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	6	48	theme	water	1095:1099	arg1	0.51					1111:1114	0.51	1111:1114	0.51	1111:1114	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	6	48	theme	water	1095:1099	arg1	activity					1101:1108	water activity	1095:1108	water activity (0.51)	1095:1115	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	4	49	theme	inlet	617:621	arg1	parameters					577:586	independent parameters	565:586	independent parameters	565:586	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	49	theme	inlet	617:621	arg1	temperature					627:637	inlet air temperature	617:637	inlet air temperature	617:637	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	1	50	from	bran	210:213	arg1	extract					185:191	anthocyanin extract	173:191	anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder	173:286	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	3	51	theme	spray	464:468	arg1	dryer					470:474	a spray dryer	462:474	a spray dryer	462:474	The microencapsulation was carried out in a spray dryer, and the process was optimized using a rotatable central composite design.					
30500502	5	52	theme	spray	882:886	arg1	parameters					903:912	the spray drying process parameters	878:912	the spray drying process parameters	878:912	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	6	53	theme	spray-dried	1046:1056	arg1	powders					1058:1064	spray-dried powders	1046:1064	spray-dried powders	1046:1064	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	7	54	theme	microencapsulated	1295:1311	arg1	particles					1313:1321	the microencapsulated particles	1291:1321	the microencapsulated particles	1291:1321	The thermal, crystallinity and surface morphology of the microencapsulated particles were also comprehensively studied using DSC, XRD, FTIR, and SEM.					
30500502	9	55	theme	steady-shear	1559:1570	arg1	rheology					1572:1579	the steady-shear rheology	1555:1579	the steady-shear rheology of the rice dough	1555:1597	The microencapsulated particles also have an effect on the steady-shear rheology of the rice dough.					
30500502	0	56	theme	rice	54:57	arg1	bran					59:62	purple rice bran	47:62	purple rice bran using modified rice starch and its effect on rice dough rheology	47:127	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	1	57	with	bran	210:213	arg1	starch					244:249	modified glutinous rice starch	220:249	modified glutinous rice starch	220:249	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	8	58	theme	storage	1392:1398	arg1	stability					1400:1408	The storage stability	1388:1408	The storage stability of microencapsulated anthocyanin	1388:1441	The storage stability of microencapsulated anthocyanin showed more stability at 4 °C than at 25 °C for 90 days.					
30500502	4	59	theme	water	822:826	arg1	activity					828:835	water activity	822:835	water activity	822:835	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	9	60	theme	dough	1593:1597	arg1	rheology					1572:1579	the steady-shear rheology	1555:1579	the steady-shear rheology of the rice dough	1555:1597	The microencapsulated particles also have an effect on the steady-shear rheology of the rice dough.					
30500502	2	61	theme	wall	336:339	arg1	material					341:348	wall material	336:348	wall material where anthocyanin extract was a core material for microencapsulation	336:417	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	2	61	theme	wall	336:339	arg1	starch					317:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch	289:322	The modified glutinous rice starch was used as wall material where anthocyanin extract was a core material for microencapsulation.					
30500502	0	62	from	effect	99:104	arg1	rheology					120:127	rice dough rheology	109:127	rice dough rheology	109:127	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	5	63	theme	4.96 MPa	986:993	arg1	pressure					1004:1011	4.96 MPa atomizer pressure	986:1011	4.96 MPa atomizer pressure	986:1011	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	4	64	from	density	810:816	arg1	parameters					680:689	the dependent parameters	666:689	the dependent parameters	666:689	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	64	from	density	810:816	arg1	efficiency					714:723	encapsulation efficiency	700:723	encapsulation efficiency	700:723	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	6	65	theme	solubility	1118:1127	arg1	term					1087:1090	term	1087:1090	term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020)	1087:1235	The physical characteristics of spray-dried powders were investigated in term of water activity (0.51), solubility (51.83%), bulk density (1.404 g/cm3), porosity (0.20), and diameter (6.44 μm) and the Hausner ratio (1.020).					
30500502	4	66	from	activity	795:802	arg1	parameters					680:689	the dependent parameters	666:689	the dependent parameters	666:689	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	66	from	activity	795:802	arg1	efficiency					714:723	encapsulation efficiency	700:723	encapsulation efficiency	700:723	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	1	67	theme	modified	220:227	arg1	starch					244:249	modified glutinous rice starch	220:249	modified glutinous rice starch	220:249	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	4	68	theme	DPPH	778:781	arg1	activity					795:802	2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity	747:802	2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity	747:802	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	69	theme	scavenging	784:793	arg1	activity					795:802	2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity	747:802	2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity	747:802	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	8	70	theme	microencapsulated	1413:1429	arg1	anthocyanin					1431:1441	microencapsulated anthocyanin	1413:1441	microencapsulated anthocyanin	1413:1441	The storage stability of microencapsulated anthocyanin showed more stability at 4 °C than at 25 °C for 90 days.					
30500502	4	71	theme	encapsulation	700:712	arg1	efficiency					714:723	encapsulation efficiency	700:723	encapsulation efficiency	700:723	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	9	72	theme	rice	1588:1591	arg1	dough					1593:1597	the rice dough	1584:1597	the rice dough	1584:1597	The microencapsulated particles also have an effect on the steady-shear rheology of the rice dough.					
30500502	1	73	theme	glutinous	229:237	arg1	starch					244:249	modified glutinous rice starch	220:249	modified glutinous rice starch	220:249	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	0	74	theme	modified	70:77	arg1	starch					84:89	modified rice starch	70:89	modified rice starch	70:89	Microencapsulation of anthocyanin extract from purple rice bran using modified rice starch and its effect on rice dough rheology.					
30500502	5	75	theme	drying	888:893	arg1	parameters					903:912	the spray drying process parameters	878:912	the spray drying process parameters	878:912	The optimum values of the spray drying process parameters were 6.01% of starch concentration, 168.78 °C inlet air temperature and 4.96 MPa atomizer pressure.					
30500502	4	76	theme	true	805:808	arg1	density					810:816	true density	805:816	true density	805:816	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	77	theme	dependent	670:678	arg1	parameters					680:689	the dependent parameters	666:689	the dependent parameters	666:689	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	77	theme	dependent	670:678	arg1	efficiency					714:723	encapsulation efficiency	700:723	encapsulation efficiency	700:723	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	78	theme	atomizer	644:651	arg1	pressure					653:660	atomizer pressure	644:660	atomizer pressure	644:660	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	78	theme	atomizer	644:651	arg1	parameters					577:586	independent parameters	565:586	independent parameters	565:586	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	1	79	theme	rice	239:242	arg1	starch					244:249	modified glutinous rice starch	220:249	modified glutinous rice starch	220:249	The investigation aims to microencapsulate anthocyanin extract from purple rice bran with modified glutinous rice starch to provide stable anthocyanin powder.					
30500502	8	80	theme	anthocyanin	1431:1441	arg1	stability					1400:1408	The storage stability	1388:1408	The storage stability of microencapsulated anthocyanin	1388:1441	The storage stability of microencapsulated anthocyanin showed more stability at 4 °C than at 25 °C for 90 days.					
30500502	9	81	theme	microencapsulated	1504:1520	arg1	particles					1522:1530	The microencapsulated particles	1500:1530	The microencapsulated particles	1500:1530	The microencapsulated particles also have an effect on the steady-shear rheology of the rice dough.					
30500502	4	82	theme	starch	595:600	arg1	concentration					602:614	starch concentration	595:614	starch concentration	595:614	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	4	82	theme	starch	595:600	arg1	parameters					577:586	independent parameters	565:586	independent parameters	565:586	The effect of independent parameters, viz., starch concentration, inlet air temperature, and atomizer pressure, on the dependent parameters, such as encapsulation efficiency, anthocyanin content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, true density and water activity were investigated.					
30500502	3	83	theme	rotatable	515:523	arg1	design					543:548	a rotatable central composite design	513:548	a rotatable central composite design	513:548	The microencapsulation was carried out in a spray dryer, and the process was optimized using a rotatable central composite design.					
30500502	9	84	contain	have	1537:1540	arg2	effect					1545:1550	an effect	1542:1550	an effect on the steady-shear rheology of the rice dough	1542:1597	The microencapsulated particles also have an effect on the steady-shear rheology of the rice dough.					
30500502	9	84	contain	have	1537:1540	arg1	particles					1522:1530	The microencapsulated particles	1500:1530	The microencapsulated particles	1500:1530	The microencapsulated particles also have an effect on the steady-shear rheology of the rice dough.					
30872056	0	0	theme	carbon	81:86	arg1	g-C3N4					97:102	g-C3N4	97:102	g-C3N4	97:102	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	0	0	theme	carbon	81:86	arg1	nitride					88:94	metal free heterogeneous graphitic carbon nitride	46:94	metal free heterogeneous graphitic carbon nitride (g-C3N4)	46:103	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	2	1	theme	pesticides	506:515	arg1	class					481:485	a class	479:485	a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS)	479:555	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	9	2	theme	electron-hole	1584:1596	arg1	pairs					1598:1602	electron-hole pairs	1584:1602	electron-hole pairs	1584:1602	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	1	3	theme	pesticide	255:263	arg1	degradation					229:239	the photocatalytic degradation	210:239	the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst	210:369	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	0	4	theme	graphitic	71:79	arg1	g-C3N4					97:102	g-C3N4	97:102	g-C3N4	97:102	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	0	4	theme	graphitic	71:79	arg1	nitride					88:94	metal free heterogeneous graphitic carbon nitride	46:94	metal free heterogeneous graphitic carbon nitride (g-C3N4)	46:103	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	3	5	dep	pests	609:613	arg1	to					598:599	to	598:599	to	598:599	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	2	6	with	pesticides	506:515	arg1	formula					533:539	a chemical formula	522:539	a chemical formula (C9H11Cl3NO3PS)	522:555	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	2	6	with	pesticides	506:515	arg1	C9H11Cl3NO3PS					542:554	C9H11Cl3NO3PS	542:554	C9H11Cl3NO3PS	542:554	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	0	7	dep	incorporated	105:116	arg1	Photocatalytic					140:153	Photocatalytic	140:153	Photocatalytic	140:153	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	0	7	dep	incorporated	105:116	arg1	studies					170:176	adsorption studies	159:176	adsorption studies	159:176	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	5	8	theme	Electron	1069:1076	arg1	ESR					1094:1096	ESR	1094:1096	ESR	1094:1096	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	8	theme	Electron	1069:1076	arg1	resonance					1083:1091	Electron spin resonance	1069:1091	Electron spin resonance (ESR)	1069:1097	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	6	9	theme	good	1152:1155	arg1	efficiency					1157:1166	good efficiency	1152:1166	good efficiency of about 85%	1152:1179	The degradation of pesticide using CS/g-C3N4 showed good efficiency of about 85%.					
30872056	5	10	theme	Diffuse	1031:1037	arg1	spectra					1051:1057	Diffuse reflectance spectra	1031:1057	Diffuse reflectance spectra (DRS)	1031:1063	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	10	theme	Diffuse	1031:1037	arg1	DRS					1060:1062	DRS	1060:1062	DRS	1060:1062	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	11	theme	Thermal	948:954	arg1	TGA-DSC					966:972	TGA-DSC	966:972	TGA-DSC	966:972	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	11	theme	Thermal	948:954	arg1	analysis					956:963	Thermal analysis	948:963	Thermal analysis (TGA-DSC)	948:973	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	7	12	from	treatment	1257:1265	arg1	solution					1294:1301	aqueous solution	1286:1301	aqueous solution	1286:1301	The results suggest that CS/g-C3N4 composite is a good alternative for the treatment of chlorpyrifos in aqueous solution.					
30872056	1	13	theme	metal	273:277	arg1	g-C3N4					324:329	g-C3N4	324:329	g-C3N4	324:329	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	1	13	theme	metal	273:277	arg1	nitride					315:321	a metal free heterogeneous graphitic carbon nitride	271:321	a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan	271:357	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	5	14	theme	CS/g-C3N4	739:747	arg1	materials					749:757	The CS/g-C3N4 materials	735:757	The CS/g-C3N4 materials	735:757	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	15	theme	reflectance	1039:1049	arg1	spectra					1051:1057	Diffuse reflectance spectra	1031:1057	Diffuse reflectance spectra (DRS)	1031:1063	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	15	theme	reflectance	1039:1049	arg1	DRS					1060:1062	DRS	1060:1062	DRS	1060:1062	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	9	16	theme	Z-scheme	1614:1621	arg1	mechanism					1623:1631	a Z-scheme mechanism	1612:1631	a Z-scheme mechanism	1612:1631	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	5	17	theme	Scanning	872:879	arg1	SEM					902:904	SEM	902:904	SEM	902:904	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	17	theme	Scanning	872:879	arg1	microscope					890:899	Scanning electron microscope	872:899	Scanning electron microscope (SEM)	872:905	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	2	18	theme	phosphorothioate	426:441	arg1	CPFS					444:447	O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)	389:448	O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)	389:448	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	2	18	theme	phosphorothioate	426:441	arg1	O					386:386	O	386:386	O	386:386	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	2	19	theme	chemical	524:531	arg1	formula					533:539	a chemical formula	522:539	a chemical formula (C9H11Cl3NO3PS)	522:555	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	2	19	theme	chemical	524:531	arg1	C9H11Cl3NO3PS					542:554	C9H11Cl3NO3PS	542:554	C9H11Cl3NO3PS	542:554	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	6	20	theme	%	1179:1179	arg1	efficiency					1157:1166	good efficiency	1152:1166	good efficiency of about 85%	1152:1179	The degradation of pesticide using CS/g-C3N4 showed good efficiency of about 85%.					
30872056	2	21	dep	Chlorpyrifos	372:383	arg1	CPFS					444:447	O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)	389:448	O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)	389:448	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	2	21	dep	Chlorpyrifos	372:383	arg1	O					386:386	O	386:386	O	386:386	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	7	22	from	chlorpyrifos	1270:1281	arg1	solution					1294:1301	aqueous solution	1286:1301	aqueous solution	1286:1301	The results suggest that CS/g-C3N4 composite is a good alternative for the treatment of chlorpyrifos in aqueous solution.					
30872056	2	23	theme	O-diethylO-3,5,6-trichloro-2-pyridyl	389:424	arg1	CPFS					444:447	O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)	389:448	O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)	389:448	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	2	23	theme	O-diethylO-3,5,6-trichloro-2-pyridyl	389:424	arg1	O					386:386	O	386:386	O	386:386	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	2	24	theme	Organophosphate	490:504	arg1	pesticides					506:515	Organophosphate pesticides	490:515	Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS)	490:555	Chlorpyrifos (O, O-diethylO-3,5,6-trichloro-2-pyridyl phosphorothioate (CPFS)), an insecticide includes in a class of Organophosphate pesticides with a chemical formula (C9H11Cl3NO3PS).					
30872056	9	25	theme	pairs	1598:1602	arg1	separation					1570:1579	an efficient separation	1557:1579	an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers	1557:1686	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	8	26	theme	electron	1476:1483	arg1	carrier					1485:1491	an electron carrier	1473:1491	an electron carrier	1473:1491	We have proposed a novel photocatalyst by metal free heterogeneous graphitic carbon using melamine and its recombination effects were minimized by chitosan which act as an electron carrier.					
30872056	8	26	theme	electron	1476:1483	arg1	chitosan					1451:1458	chitosan	1451:1458	chitosan which act as an electron carrier	1451:1491	We have proposed a novel photocatalyst by metal free heterogeneous graphitic carbon using melamine and its recombination effects were minimized by chitosan which act as an electron carrier.					
30872056	4	27	theme	chlorpyrifos	670:681	arg1	dangerous					692:700	dangerous	692:700	dangerous to all aquatic living organisms	692:732	The acute toxicity of chlorpyrifos is still dangerous to all aquatic living organisms.					
30872056	4	27	theme	chlorpyrifos	670:681	arg1	toxicity					658:665	The acute toxicity	648:665	The acute toxicity of chlorpyrifos	648:681	The acute toxicity of chlorpyrifos is still dangerous to all aquatic living organisms.					
30872056	1	28	theme	free	279:282	arg1	g-C3N4					324:329	g-C3N4	324:329	g-C3N4	324:329	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	1	28	theme	free	279:282	arg1	nitride					315:321	a metal free heterogeneous graphitic carbon nitride	271:321	a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan	271:357	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	5	29	dep	spectrophotometer	796:812	arg1	UV-vis					815:820	UV-vis	815:820	UV-vis	815:820	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	3	30	theme	agricultural	579:590	arg1	pests					609:613	agricultural lands to control pests	579:613	agricultural lands to control pests in cotton	579:623	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	3	30	theme	agricultural	579:590	arg1	vegetables					636:645	vegetables	636:645	vegetables	636:645	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	3	30	theme	agricultural	579:590	arg1	fruit					626:630	fruit	626:630	fruit	626:630	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	9	31	theme	enhanced	1498:1505	arg1	performance					1522:1532	The enhanced photocatalytic performance	1494:1532	The enhanced photocatalytic performance	1494:1532	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	1	32	theme	present	183:189	arg1	study					191:195	The present study	179:195	The present study	179:195	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	7	33	theme	aqueous	1286:1292	arg1	solution					1294:1301	aqueous solution	1286:1301	aqueous solution	1286:1301	The results suggest that CS/g-C3N4 composite is a good alternative for the treatment of chlorpyrifos in aqueous solution.					
30872056	1	34	theme	heterogeneous	284:296	arg1	g-C3N4					324:329	g-C3N4	324:329	g-C3N4	324:329	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	1	34	theme	heterogeneous	284:296	arg1	nitride					315:321	a metal free heterogeneous graphitic carbon nitride	271:321	a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan	271:357	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	5	35	theme	X-ray	926:930	arg1	EDX					942:944	EDX	942:944	EDX	942:944	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	35	theme	X-ray	926:930	arg1	Analysis					932:939	Energy Dispersive X-ray Analysis	908:939	Energy Dispersive X-ray Analysis (EDX)	908:945	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	9	36	theme	photocatalytic	1507:1520	arg1	performance					1522:1532	The enhanced photocatalytic performance	1494:1532	The enhanced photocatalytic performance	1494:1532	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	5	37	theme	oxygen	985:990	arg1	demand					992:997	Chemical oxygen demand	976:997	Chemical oxygen demand (COD)	976:1003	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	37	theme	oxygen	985:990	arg1	COD					1000:1002	COD	1000:1002	COD	1000:1002	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	3	38	used	used	571:574	arg2	It					558:559	It	558:559	It	558:559	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	0	39	theme	chlorpyrifos	11:22	arg1	Removal					0:6	Removal	0:6	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4)	0:103	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	1	40	theme	graphitic	298:306	arg1	g-C3N4					324:329	g-C3N4	324:329	g-C3N4	324:329	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	1	40	theme	graphitic	298:306	arg1	nitride					315:321	a metal free heterogeneous graphitic carbon nitride	271:321	a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan	271:357	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	8	41	theme	graphitic	1371:1379	arg1	carbon					1381:1386	metal free heterogeneous graphitic carbon	1346:1386	metal free heterogeneous graphitic carbon using melamine and its recombination effects	1346:1431	We have proposed a novel photocatalyst by metal free heterogeneous graphitic carbon using melamine and its recombination effects were minimized by chitosan which act as an electron carrier.					
30872056	7	42	theme	good	1232:1235	arg1	alternative					1237:1247	a good alternative	1230:1247	a good alternative for the treatment of chlorpyrifos in aqueous solution	1230:1301	The results suggest that CS/g-C3N4 composite is a good alternative for the treatment of chlorpyrifos in aqueous solution.					
30872056	7	42	theme	good	1232:1235	arg1	composite					1217:1225	CS/g-C3N4 composite	1207:1225	CS/g-C3N4 composite	1207:1225	The results suggest that CS/g-C3N4 composite is a good alternative for the treatment of chlorpyrifos in aqueous solution.					
30872056	8	43	theme	free	1352:1355	arg1	carbon					1381:1386	metal free heterogeneous graphitic carbon	1346:1386	metal free heterogeneous graphitic carbon using melamine and its recombination effects	1346:1431	We have proposed a novel photocatalyst by metal free heterogeneous graphitic carbon using melamine and its recombination effects were minimized by chitosan which act as an electron carrier.					
30872056	5	44	theme	UV-vis	789:794	arg1	spectrophotometer					796:812	UV-vis spectrophotometer	789:812	UV-vis spectrophotometer (UV-vis)	789:821	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	1	45	theme	carbon	308:313	arg1	g-C3N4					324:329	g-C3N4	324:329	g-C3N4	324:329	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	1	45	theme	carbon	308:313	arg1	nitride					315:321	a metal free heterogeneous graphitic carbon nitride	271:321	a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan	271:357	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	8	46	theme	heterogeneous	1357:1369	arg1	carbon					1381:1386	metal free heterogeneous graphitic carbon	1346:1386	metal free heterogeneous graphitic carbon using melamine and its recombination effects	1346:1431	We have proposed a novel photocatalyst by metal free heterogeneous graphitic carbon using melamine and its recombination effects were minimized by chitosan which act as an electron carrier.					
30872056	3	47	theme	control	601:607	arg1	pests					609:613	agricultural lands to control pests	579:613	agricultural lands to control pests in cotton	579:623	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	3	47	theme	control	601:607	arg1	vegetables					636:645	vegetables	636:645	vegetables	636:645	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	3	47	theme	control	601:607	arg1	fruit					626:630	fruit	626:630	fruit	626:630	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	3	48	from	pests	609:613	arg1	cotton					618:623	cotton	618:623	cotton	618:623	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	7	49	theme	CS/g-C3N4	1207:1215	arg1	alternative					1237:1247	a good alternative	1230:1247	a good alternative for the treatment of chlorpyrifos in aqueous solution	1230:1301	The results suggest that CS/g-C3N4 composite is a good alternative for the treatment of chlorpyrifos in aqueous solution.					
30872056	7	49	theme	CS/g-C3N4	1207:1215	arg1	composite					1217:1225	CS/g-C3N4 composite	1207:1225	CS/g-C3N4 composite	1207:1225	The results suggest that CS/g-C3N4 composite is a good alternative for the treatment of chlorpyrifos in aqueous solution.					
30872056	5	50	theme	spin	1078:1081	arg1	ESR					1094:1096	ESR	1094:1096	ESR	1094:1096	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	50	theme	spin	1078:1081	arg1	resonance					1083:1091	Electron spin resonance	1069:1091	Electron spin resonance (ESR)	1069:1097	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	9	51	theme	efficient	1560:1568	arg1	separation					1570:1579	an efficient separation	1557:1579	an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers	1557:1686	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	1	52	theme	photocatalytic	214:227	arg1	degradation					229:239	the photocatalytic degradation	210:239	the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst	210:369	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30872056	0	53	theme	metal	46:50	arg1	g-C3N4					97:102	g-C3N4	97:102	g-C3N4	97:102	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	0	53	theme	metal	46:50	arg1	nitride					88:94	metal free heterogeneous graphitic carbon nitride	46:94	metal free heterogeneous graphitic carbon nitride (g-C3N4)	46:103	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	9	54	theme	charge	1661:1666	arg1	carriers					1679:1686	charge separation carriers	1661:1686	charge separation carriers	1661:1686	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	9	54	theme	charge	1661:1666	arg1	chitosan					1643:1650	chitosan	1643:1650	chitosan	1643:1650	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	3	55	theme	lands	592:596	arg1	pests					609:613	agricultural lands to control pests	579:613	agricultural lands to control pests in cotton	579:623	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	3	55	theme	lands	592:596	arg1	vegetables					636:645	vegetables	636:645	vegetables	636:645	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	3	55	theme	lands	592:596	arg1	fruit					626:630	fruit	626:630	fruit	626:630	It is widely used in agricultural lands to control pests in cotton, fruit and vegetables.					
30872056	4	56	theme	aquatic	709:715	arg1	organisms					724:732	all aquatic living organisms	705:732	all aquatic living organisms	705:732	The acute toxicity of chlorpyrifos is still dangerous to all aquatic living organisms.					
30872056	8	57	theme	novel	1323:1327	arg1	photocatalyst					1329:1341	a novel photocatalyst	1321:1341	a novel photocatalyst by metal free heterogeneous graphitic carbon using melamine and its recombination effects	1321:1431	We have proposed a novel photocatalyst by metal free heterogeneous graphitic carbon using melamine and its recombination effects were minimized by chitosan which act as an electron carrier.					
30872056	9	58	theme	separation	1668:1677	arg1	carriers					1679:1686	charge separation carriers	1661:1686	charge separation carriers	1661:1686	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	9	58	theme	separation	1668:1677	arg1	chitosan					1643:1650	chitosan	1643:1650	chitosan	1643:1650	The enhanced photocatalytic performance could be attributed to an efficient separation of electron-hole pairs through a Z-scheme mechanism, in which chitosan acted as charge separation carriers.					
30872056	5	59	theme	Energy	908:913	arg1	EDX					942:944	EDX	942:944	EDX	942:944	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	59	theme	Energy	908:913	arg1	Analysis					932:939	Energy Dispersive X-ray Analysis	908:939	Energy Dispersive X-ray Analysis (EDX)	908:945	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	8	60	theme	metal	1346:1350	arg1	carbon					1381:1386	metal free heterogeneous graphitic carbon	1346:1386	metal free heterogeneous graphitic carbon using melamine and its recombination effects	1346:1431	We have proposed a novel photocatalyst by metal free heterogeneous graphitic carbon using melamine and its recombination effects were minimized by chitosan which act as an electron carrier.					
30872056	0	61	theme	adsorption	159:168	arg1	studies					170:176	adsorption studies	159:176	adsorption studies	159:176	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	7	62	theme	chlorpyrifos	1270:1281	arg1	treatment					1257:1265	the treatment	1253:1265	the treatment of chlorpyrifos in aqueous solution	1253:1301	The results suggest that CS/g-C3N4 composite is a good alternative for the treatment of chlorpyrifos in aqueous solution.					
30872056	6	63	theme	CS/g-C3N4	1135:1143	arg1	degradation					1104:1114	The degradation	1100:1114	The degradation of pesticide using CS/g-C3N4	1100:1143	The degradation of pesticide using CS/g-C3N4 showed good efficiency of about 85%.					
30872056	5	64	theme	Dispersive	915:924	arg1	EDX					942:944	EDX	942:944	EDX	942:944	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	64	theme	Dispersive	915:924	arg1	Analysis					932:939	Energy Dispersive X-ray Analysis	908:939	Energy Dispersive X-ray Analysis (EDX)	908:945	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	65	theme	electron	881:888	arg1	SEM					902:904	SEM	902:904	SEM	902:904	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	65	theme	electron	881:888	arg1	microscope					890:899	Scanning electron microscope	872:899	Scanning electron microscope (SEM)	872:905	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	66	theme	X-ray	1006:1010	arg1	XRD					1025:1027	XRD	1025:1027	XRD	1025:1027	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	66	theme	X-ray	1006:1010	arg1	diffraction					1012:1022	X-ray diffraction	1006:1022	X-ray diffraction (XRD)	1006:1028	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	6	67	theme	using	1129:1133	arg1	CS/g-C3N4					1135:1143	pesticide using CS/g-C3N4	1119:1143	pesticide using CS/g-C3N4	1119:1143	The degradation of pesticide using CS/g-C3N4 showed good efficiency of about 85%.					
30872056	4	68	theme	living	717:722	arg1	organisms					724:732	all aquatic living organisms	705:732	all aquatic living organisms	705:732	The acute toxicity of chlorpyrifos is still dangerous to all aquatic living organisms.					
30872056	0	69	theme	heterogeneous	57:69	arg1	g-C3N4					97:102	g-C3N4	97:102	g-C3N4	97:102	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	0	69	theme	heterogeneous	57:69	arg1	nitride					88:94	metal free heterogeneous graphitic carbon nitride	46:94	metal free heterogeneous graphitic carbon nitride (g-C3N4)	46:103	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	6	70	theme	pesticide	1119:1127	arg1	CS/g-C3N4					1135:1143	pesticide using CS/g-C3N4	1119:1143	pesticide using CS/g-C3N4	1119:1143	The degradation of pesticide using CS/g-C3N4 showed good efficiency of about 85%.					
30872056	5	71	theme	infrared	842:849	arg1	FTIR					865:868	FTIR	865:868	FTIR	865:868	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	71	theme	infrared	842:849	arg1	spectroscopy					851:862	infrared spectroscopy	842:862	infrared spectroscopy (FTIR)	842:869	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	4	72	theme	acute	652:656	arg1	dangerous					692:700	dangerous	692:700	dangerous to all aquatic living organisms	692:732	The acute toxicity of chlorpyrifos is still dangerous to all aquatic living organisms.					
30872056	4	72	theme	acute	652:656	arg1	toxicity					658:665	The acute toxicity	648:665	The acute toxicity of chlorpyrifos	648:681	The acute toxicity of chlorpyrifos is still dangerous to all aquatic living organisms.					
30872056	0	73	theme	free	52:55	arg1	g-C3N4					97:102	g-C3N4	97:102	g-C3N4	97:102	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	0	73	theme	free	52:55	arg1	nitride					88:94	metal free heterogeneous graphitic carbon nitride	46:94	metal free heterogeneous graphitic carbon nitride (g-C3N4)	46:103	Removal of chlorpyrifos, an insecticide using metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated chitosan as catalyst: Photocatalytic and adsorption studies.					
30872056	5	74	theme	Chemical	976:983	arg1	demand					992:997	Chemical oxygen demand	976:997	Chemical oxygen demand (COD)	976:1003	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	5	74	theme	Chemical	976:983	arg1	COD					1000:1002	COD	1000:1002	COD	1000:1002	The CS/g-C3N4 materials have been characterized using UV-vis spectrophotometer (UV-vis), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Energy Dispersive X-ray Analysis (EDX), Thermal analysis (TGA-DSC), Chemical oxygen demand (COD), X-ray diffraction (XRD), Diffuse reflectance spectra (DRS) and Electron spin resonance (ESR).					
30872056	7	75	from	solution	1294:1301	arg1	treatment					1257:1265	the treatment	1253:1265	the treatment of chlorpyrifos in aqueous solution	1253:1301	The results suggest that CS/g-C3N4 composite is a good alternative for the treatment of chlorpyrifos in aqueous solution.					
30872056	8	76	theme	recombination	1411:1423	arg1	effects					1425:1431	its recombination effects	1407:1431	its recombination effects	1407:1431	We have proposed a novel photocatalyst by metal free heterogeneous graphitic carbon using melamine and its recombination effects were minimized by chitosan which act as an electron carrier.					
30872056	1	77	theme	organic	247:253	arg1	pesticide					255:263	an organic pesticide	244:263	an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst	244:369	The present study investigates the photocatalytic degradation of an organic pesticide using a metal free heterogeneous graphitic carbon nitride (g-C3N4) incorporated into chitosan as catalyst.					
30829000	10	0	from	intestine	1800:1808	arg1	samples					1777:1783	samples	1777:1783	samples from the small intestine	1777:1808	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	12	1	theme	significant	1977:1987	arg1	differences					1989:1999	significant differences	1977:1999	significant differences in composition and potential function among the bacterial communities in the goat GIT	1977:2085	In general, there were significant differences in composition and potential function among the bacterial communities in the goat GIT.					
30829000	4	2	theme	hypervariable	716:728	arg1	region					730:735	the hypervariable region	712:735	the hypervariable region of the 16S rRNA gene	712:756	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	2	3	theme	Little	361:366	arg1	information					368:378	Little information	361:378	Little information	361:378	Little information has been reported on these aspects of the goat GIT.					
30829000	1	4	theme	nutritionists	255:267	arg1	attention					242:250	the attention	238:250	the attention of nutritionists	238:267	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	5	from	function	139:146	arg1	GIT					206:208	GIT	206:208	GIT	206:208	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	5	from	function	139:146	arg1	tract					199:203	the gastrointestinal tract	178:203	the gastrointestinal tract (GIT)	178:209	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	10	6	theme	glycan	1735:1740	arg1	metabolism					1742:1751	glycan metabolism	1735:1751	glycan metabolism	1735:1751	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	4	7	theme	Total	621:625	arg1	DNA					635:637	Total genomic DNA	621:637	Total genomic DNA	621:637	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	4	8	theme	universal	795:803	arg1	primers					805:811	bacterial universal primers	785:811	bacterial universal primers	785:811	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	3	9	theme	microbiota	559:568	arg1	composition					527:537	composition	527:537	composition	527:537	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	3	9	theme	microbiota	559:568	arg1	function					543:550	function	543:550	function	543:550	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	11	10	from	levels	1885:1890	arg1	abundances					1838:1847	the relative abundances	1825:1847	the relative abundances of bacteria at the phylum and genus levels	1825:1890	Additionally, the relative abundances of bacteria at the phylum and genus levels were significantly correlated with these metabolic functions.					
30829000	10	11	theme	energy	1527:1532	arg1	metabolism					1534:1543	energy metabolism	1527:1543	energy metabolism	1527:1543	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	6	12	theme	goat	1013:1016	arg1	GIT					1018:1020	the goat GIT	1009:1020	the goat GIT	1009:1020	A total of 857 genera that belonged to 39 phyla were observed across the goat GIT, with Bacteroidetes and Firmicutes dominating.					
30829000	9	13	theme	microbial	1425:1433	arg1	function					1435:1442	microbial function	1425:1442	microbial function	1425:1442	The study indicated that there were significant differences in microbial function among the GIT regions.					
30829000	10	14	theme	acid	1552:1555	arg1	metabolism					1557:1566	amino acid metabolism	1546:1566	amino acid metabolism	1546:1566	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	5	15	theme	MiSeq	858:862	arg1	platform					864:871	an Illumina MiSeq platform	846:871	an Illumina MiSeq platform	846:871	The amplicons were sequenced on an Illumina MiSeq platform, and the biological information was analyzed using QIIME software.					
30829000	11	16	theme	metabolic	1933:1941	arg1	functions					1943:1951	these metabolic functions	1927:1951	these metabolic functions	1927:1951	Additionally, the relative abundances of bacteria at the phylum and genus levels were significantly correlated with these metabolic functions.					
30829000	9	17	theme	GIT	1454:1456	arg1	regions					1458:1464	the GIT regions	1450:1464	the GIT regions	1450:1464	The study indicated that there were significant differences in microbial function among the GIT regions.					
30829000	6	18	theme	genera	955:960	arg1	total					942:946	A total	940:946	A total of 857 genera that belonged to 39 phyla	940:986	A total of 857 genera that belonged to 39 phyla were observed across the goat GIT, with Bacteroidetes and Firmicutes dominating.					
30829000	11	19	theme	phylum	1868:1873	arg1	levels					1885:1890	the phylum and genus levels	1864:1890	levels	1885:1890	Additionally, the relative abundances of bacteria at the phylum and genus levels were significantly correlated with these metabolic functions.					
30829000	4	20	theme	chyme	658:662	arg1	samples					664:670	chyme samples	658:670	chyme samples from different sections of the GIT	658:705	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	7	21	theme	species	1149:1155	arg1	abundance					1157:1165	the composition, diversity, and species abundance	1117:1165	the composition, diversity, and species abundance of the bacterial communities	1117:1194	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	1	22	theme	animals	352:358	arg1	efficiency					322:331	the nutrient utilization efficiency	297:331	the nutrient utilization efficiency	297:331	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	22	theme	animals	352:358	arg1	health					337:342	health	337:342	health	337:342	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	23	theme	community	165:173	arg1	function					139:146	function	139:146	function	139:146	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	23	theme	community	165:173	arg1	composition					123:133	composition	123:133	composition	123:133	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	8	24	theme	bacterial	1267:1275	arg1	communities					1277:1287	the bacterial communities	1263:1287	the bacterial communities	1263:1287	However, the compositions of the bacterial communities in adjacent GIT segments showed similarities in addition to differences.					
30829000	7	25	from	differences	1102:1112	arg1	abundance					1157:1165	the composition, diversity, and species abundance	1117:1165	the composition, diversity, and species abundance of the bacterial communities	1117:1194	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	7	25	from	differences	1102:1112	arg1	sections					1213:1220	the different sections	1199:1220	the different sections of the GIT	1199:1231	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	3	26	used	used	443:446	arg2	study					437:441	This study	432:441	This study	432:441	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	10	27	theme	glycan	1596:1601	arg1	metabolism					1603:1612	glycan metabolism	1596:1612	glycan metabolism	1596:1612	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	5	28	theme	QIIME	924:928	arg1	software					930:937	QIIME software	924:937	QIIME software	924:937	The amplicons were sequenced on an Illumina MiSeq platform, and the biological information was analyzed using QIIME software.					
30829000	8	29	theme	GIT	1301:1303	arg1	segments					1305:1312	adjacent GIT segments	1292:1312	adjacent GIT segments	1292:1312	However, the compositions of the bacterial communities in adjacent GIT segments showed similarities in addition to differences.					
30829000	11	30	theme	relative	1829:1836	arg1	abundances					1838:1847	the relative abundances	1825:1847	the relative abundances of bacteria at the phylum and genus levels	1825:1890	Additionally, the relative abundances of bacteria at the phylum and genus levels were significantly correlated with these metabolic functions.					
30829000	12	31	from	communities	2059:2069	arg1	GIT					2083:2085	the goat GIT	2074:2085	the goat GIT	2074:2085	In general, there were significant differences in composition and potential function among the bacterial communities in the goat GIT.					
30829000	2	32	theme	GIT	427:429	arg1	aspects					407:413	these aspects	401:413	these aspects of the goat GIT	401:429	Little information has been reported on these aspects of the goat GIT.					
30829000	0	33	theme	bacterial	19:27	arg1	community					29:37	the bacterial community	15:37	the bacterial community across the gastrointestinal tract of goats	15:80	Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function.					
30829000	10	34	theme	small	1794:1798	arg1	intestine					1800:1808	the small intestine	1790:1808	the small intestine	1790:1808	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	1	35	theme	utilization	310:320	arg1	efficiency					322:331	the nutrient utilization efficiency	297:331	the nutrient utilization efficiency	297:331	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	7	36	theme	GIT	1229:1231	arg1	sections					1213:1220	the different sections	1199:1220	the different sections of the GIT	1199:1231	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	0	37	theme	gastrointestinal	50:65	arg1	tract					67:71	the gastrointestinal tract	46:71	the gastrointestinal tract of goats	46:80	Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function.					
30829000	6	38	theme	Firmicutes	1046:1055	arg1	dominating					1057:1066	Firmicutes dominating	1046:1066	Firmicutes dominating	1046:1066	A total of 857 genera that belonged to 39 phyla were observed across the goat GIT, with Bacteroidetes and Firmicutes dominating.					
30829000	7	39	theme	different	1203:1211	arg1	sections					1213:1220	the different sections	1199:1220	the different sections of the GIT	1199:1231	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	4	40	theme	rRNA	748:751	arg1	gene					753:756	the 16S rRNA gene	740:756	the 16S rRNA gene	740:756	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	10	41	theme	related	1678:1684	arg1	genes					1672:1676	genes	1672:1676	genes related to energy metabolism, amino acid metabolism, and glycan metabolism	1672:1751	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	0	42	dep	Characterizing	0:13	arg1	Composition					83:93	Composition	83:93	Composition	83:93	Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function.					
30829000	0	42	dep	Characterizing	0:13	arg1	function					109:116	potential function	99:116	potential function	99:116	Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function.					
30829000	7	43	theme	bacterial	1174:1182	arg1	communities					1184:1194	the bacterial communities	1170:1194	the bacterial communities	1170:1194	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	10	44	from	forestomach	1655:1665	arg1	samples					1638:1644	samples	1638:1644	samples from the forestomach	1638:1665	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	10	45	theme	genes	1509:1513	arg1	abundances					1495:1504	the relative abundances	1482:1504	the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism	1482:1612	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	10	46	theme	energy	1689:1694	arg1	metabolism					1696:1705	energy metabolism	1689:1705	energy metabolism	1689:1705	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	12	47	theme	goat	2078:2081	arg1	GIT					2083:2085	the goat GIT	2074:2085	the goat GIT	2074:2085	In general, there were significant differences in composition and potential function among the bacterial communities in the goat GIT.					
30829000	10	48	theme	amino	1546:1550	arg1	metabolism					1557:1566	amino acid metabolism	1546:1566	amino acid metabolism	1546:1566	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	1	49	theme	gastrointestinal	182:197	arg1	GIT					206:208	GIT	206:208	GIT	206:208	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	49	theme	gastrointestinal	182:197	arg1	tract					199:203	the gastrointestinal tract	178:203	the gastrointestinal tract (GIT)	178:209	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	4	50	theme	bacterial	785:793	arg1	primers					805:811	bacterial universal primers	785:811	bacterial universal primers	785:811	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	4	51	theme	genomic	627:633	arg1	DNA					635:637	Total genomic DNA	621:637	Total genomic DNA	621:637	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	10	52	theme	acid	1714:1717	arg1	metabolism					1719:1728	amino acid metabolism	1708:1728	amino acid metabolism	1708:1728	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	9	53	theme	significant	1398:1408	arg1	differences					1410:1420	significant differences	1398:1420	significant differences in microbial function among the GIT regions	1398:1464	The study indicated that there were significant differences in microbial function among the GIT regions.					
30829000	12	54	theme	bacterial	2049:2057	arg1	communities					2059:2069	the bacterial communities	2045:2069	the bacterial communities in the goat GIT	2045:2085	In general, there were significant differences in composition and potential function among the bacterial communities in the goat GIT.					
30829000	10	55	theme	nucleotide	1569:1578	arg1	metabolism					1580:1589	nucleotide metabolism	1569:1589	nucleotide metabolism	1569:1589	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	3	56	dep	jejunum	594:600	arg1	abomasum					584:591	abomasum	584:591	abomasum	584:591	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	7	57	theme	diversity	1134:1142	arg1	abundance					1157:1165	the composition, diversity, and species abundance	1117:1165	the composition, diversity, and species abundance of the bacterial communities	1117:1194	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	11	58	theme	genus	1879:1883	arg1	levels					1885:1890	the phylum and genus levels	1864:1890	levels	1885:1890	Additionally, the relative abundances of bacteria at the phylum and genus levels were significantly correlated with these metabolic functions.					
30829000	5	59	theme	Illumina	849:856	arg1	platform					864:871	an Illumina MiSeq platform	846:871	an Illumina MiSeq platform	846:871	The amplicons were sequenced on an Illumina MiSeq platform, and the biological information was analyzed using QIIME software.					
30829000	12	60	theme	potential	2020:2028	arg1	function					2030:2037	potential function	2020:2037	potential function	2020:2037	In general, there were significant differences in composition and potential function among the bacterial communities in the goat GIT.					
30829000	3	61	from	function	543:550	arg1	rumen					577:581	the rumen	573:581	the rumen	573:581	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	3	62	dep	composition	527:537	arg1	the					523:525	the	523:525	the	523:525	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	4	63	theme	different	677:685	arg1	sections					687:694	different sections	677:694	different sections of the GIT	677:705	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	0	64	theme	goats	76:80	arg1	tract					67:71	the gastrointestinal tract	46:71	the gastrointestinal tract of goats	46:80	Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function.					
30829000	1	65	from	tract	199:203	arg1	function					139:146	function	139:146	function	139:146	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	65	from	tract	199:203	arg1	composition					123:133	composition	123:133	composition	123:133	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	66	theme	host	347:350	arg1	animals					352:358	host animals	347:358	host animals	347:358	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	12	67	from	differences	1989:1999	arg1	composition					2004:2014	composition	2004:2014	composition	2004:2014	In general, there were significant differences in composition and potential function among the bacterial communities in the goat GIT.					
30829000	12	67	from	differences	1989:1999	arg1	function					2030:2037	potential function	2020:2037	potential function	2020:2037	In general, there were significant differences in composition and potential function among the bacterial communities in the goat GIT.					
30829000	1	68	theme	microbial	155:163	arg1	community					165:173	the microbial community	151:173	the microbial community in the gastrointestinal tract (GIT)	151:209	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	3	69	from	composition	527:537	arg1	rumen					577:581	the rumen	573:581	the rumen	573:581	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	0	70	theme	potential	99:107	arg1	function					109:116	potential function	99:116	potential function	99:116	Characterizing the bacterial community across the gastrointestinal tract of goats: Composition and potential function.					
30829000	1	71	from	community	165:173	arg1	GIT					206:208	GIT	206:208	GIT	206:208	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	71	from	community	165:173	arg1	tract					199:203	the gastrointestinal tract	178:203	the gastrointestinal tract (GIT)	178:209	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	10	72	theme	amino	1708:1712	arg1	metabolism					1719:1728	amino acid metabolism	1708:1728	amino acid metabolism	1708:1728	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	7	73	theme	composition	1121:1131	arg1	abundance					1157:1165	the composition, diversity, and species abundance	1117:1165	the composition, diversity, and species abundance of the bacterial communities	1117:1194	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	8	74	theme	communities	1277:1287	arg1	compositions					1247:1258	the compositions	1243:1258	the compositions of the bacterial communities in adjacent GIT segments	1243:1312	However, the compositions of the bacterial communities in adjacent GIT segments showed similarities in addition to differences.					
30829000	11	75	theme	bacteria	1852:1859	arg1	abundances					1838:1847	the relative abundances	1825:1847	the relative abundances of bacteria at the phylum and genus levels	1825:1890	Additionally, the relative abundances of bacteria at the phylum and genus levels were significantly correlated with these metabolic functions.					
30829000	8	76	from	compositions	1247:1258	arg1	segments					1305:1312	adjacent GIT segments	1292:1312	adjacent GIT segments	1292:1312	However, the compositions of the bacterial communities in adjacent GIT segments showed similarities in addition to differences.					
30829000	8	77	theme	adjacent	1292:1299	arg1	segments					1305:1312	adjacent GIT segments	1292:1312	adjacent GIT segments	1292:1312	However, the compositions of the bacterial communities in adjacent GIT segments showed similarities in addition to differences.					
30829000	7	78	theme	significant	1090:1100	arg1	differences					1102:1112	significant differences	1090:1112	significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT	1090:1231	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	1	79	from	composition	123:133	arg1	GIT					206:208	GIT	206:208	GIT	206:208	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	1	79	from	composition	123:133	arg1	tract					199:203	the gastrointestinal tract	178:203	the gastrointestinal tract (GIT)	178:209	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	5	80	theme	biological	882:891	arg1	information					893:903	the biological information	878:903	the biological information	878:903	The amplicons were sequenced on an Illumina MiSeq platform, and the biological information was analyzed using QIIME software.					
30829000	4	81	theme	GIT	703:705	arg1	sections					687:694	different sections	677:694	different sections of the GIT	677:705	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	2	82	theme	goat	422:425	arg1	GIT					427:429	the goat GIT	418:429	the goat GIT	418:429	Little information has been reported on these aspects of the goat GIT.					
30829000	1	83	theme	nutrient	301:308	arg1	efficiency					322:331	the nutrient utilization efficiency	297:331	the nutrient utilization efficiency	297:331	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	4	84	theme	gene	753:756	arg1	region					730:735	the hypervariable region	712:735	the hypervariable region of the 16S rRNA gene	712:756	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	3	85	theme	female	451:456	arg1	goats					458:462	12 female goats	448:462	12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age)	448:510	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	3	86	theme	age	507:509	arg1	10 months					494:502	10 months	494:502	10 months	494:502	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	3	86	theme	age	507:509	arg1	20.70 ± 1.60 kg					474:488	20.70 ± 1.60 kg	474:488	20.70 ± 1.60 kg	474:488	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	4	87	from	sections	687:694	arg1	samples					664:670	chyme samples	658:670	chyme samples from different sections of the GIT	658:705	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
30829000	9	88	from	differences	1410:1420	arg1	function					1435:1442	microbial function	1425:1442	microbial function	1425:1442	The study indicated that there were significant differences in microbial function among the GIT regions.					
30829000	1	89	dep	composition	123:133	arg1	The					119:121	The	119:121	The	119:121	The composition and function of the microbial community in the gastrointestinal tract (GIT) have increasingly captured the attention of nutritionists because these traits affect the nutrient utilization efficiency and health of host animals.					
30829000	3	90	dep	goats	458:462	arg1	weighing					465:472	weighing	465:472	weighing 20.70 ± 1.60 kg and 10 months of age	465:509	This study used 12 female goats (weighing 20.70 ± 1.60 kg and 10 months of age) to examine the composition and function of the microbiota in the rumen, abomasum, jejunum, cecum, and colon.					
30829000	7	91	theme	communities	1184:1194	arg1	abundance					1157:1165	the composition, diversity, and species abundance	1117:1165	the composition, diversity, and species abundance of the bacterial communities	1117:1194	Our results revealed significant differences in the composition, diversity, and species abundance of the bacterial communities in the different sections of the GIT.					
30829000	10	92	theme	relative	1486:1493	arg1	abundances					1495:1504	the relative abundances	1482:1504	the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism	1482:1612	In particular, the relative abundances of genes involved in energy metabolism, amino acid metabolism, nucleotide metabolism, and glycan metabolism were overrepresented in samples from the forestomach, and genes related to energy metabolism, amino acid metabolism, and glycan metabolism were mainly enriched in samples from the small intestine.					
30829000	4	93	theme	16S	744:746	arg1	gene					753:756	the 16S rRNA gene	740:756	the 16S rRNA gene	740:756	Total genomic DNA was extracted from chyme samples from different sections of the GIT, and the hypervariable region of the 16S rRNA gene was amplified by PCR using bacterial universal primers.					
29676121	0	0	theme	Pantotrichum	82:93	arg1	products					58:65	different processed products	38:65	different processed products of Cervi Cornu Pantotrichum	38:93	[Research on chemical compositions in different processed products of Cervi Cornu Pantotrichum].					
29676121	4	1	dep	decreased	1199:1207	arg1	While					1135:1139	While	1135:1139	While	1135:1139	While the contents of fatty acids and mineral elements were not decreased in these three methods.					
29676121	1	2	theme	powder	149:154	arg1	"					155:155	"antler powder"	141:155	"antler powder"	141:155	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	5	3	theme	slices	1313:1318	arg1	"					1319:1319	"antler slices"	1305:1319	"antler slices"	1305:1319	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	1	4	theme	Cervi	212:216	arg1	CCP					238:240	CCP	238:240	CCP	238:240	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	1	4	theme	Cervi	212:216	arg1	Pantotrichum					224:235	Cervi Cornu Pantotrichum	212:235	Cervi Cornu Pantotrichum (CCP)	212:241	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	0	5	theme	Cornu	76:80	arg1	Pantotrichum					82:93	Cervi Cornu Pantotrichum	70:93	Cervi Cornu Pantotrichum	70:93	[Research on chemical compositions in different processed products of Cervi Cornu Pantotrichum].					
29676121	6	6	theme	principal	1452:1460	arg1	PCA					1482:1484	PCA	1482:1484	PCA	1482:1484	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	6	6	theme	principal	1452:1460	arg1	analysis					1472:1479	principal component analysis	1452:1479	principal component analysis (PCA)	1452:1485	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	5	7	theme	crude	1249:1253	arg1	protein					1255:1261	crude protein	1249:1261	crude protein	1249:1261	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	1	8	theme	"	155:155	arg1	compositions					125:136	the chemical compositions	112:136	the chemical compositions of "antler powder" and "antler slice"	112:174	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	5	9	theme	antler	1346:1351	arg1	"					1359:1359	"antler powder"	1345:1359	"antler powder"	1345:1359	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	1	10	theme	Cornu	218:222	arg1	CCP					238:240	CCP	238:240	CCP	238:240	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	1	10	theme	Cornu	218:222	arg1	Pantotrichum					224:235	Cervi Cornu Pantotrichum	212:235	Cervi Cornu Pantotrichum (CCP)	212:241	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	3	11	theme	nucleosides	1036:1046	arg1	contents					1048:1055	the crude protein and nucleosides contents	1014:1055	contents	1048:1055	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	4	12	theme	acids	1163:1167	arg1	contents					1145:1152	the contents	1141:1152	the contents of fatty acids and mineral elements	1141:1188	While the contents of fatty acids and mineral elements were not decreased in these three methods.					
29676121	5	13	theme	protein	1255:1261	arg1	contents					1293:1300	Polysaccharide, crude protein, amino acids, and nucleosides contents	1233:1300	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices"	1233:1319	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	1	14	theme	Pantotrichum	224:235	arg1	products					200:207	processed products	190:207	processed products of Cervi Cornu Pantotrichum (CCP)	190:241	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	5	15	theme	powder	1353:1358	arg1	"					1359:1359	"antler powder"	1345:1359	"antler powder"	1345:1359	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	3	16	theme	grinding	657:664	arg1	contents					762:769	grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents	657:769	grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents	657:769	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	5	17	theme	antler	1306:1311	arg1	"					1319:1319	"antler slices"	1305:1319	"antler slices"	1305:1319	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	2	18	theme	amino	317:321	arg1	polysaccharides					285:299	polysaccharides	285:299	polysaccharides	285:299	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	18	theme	amino	317:321	arg1	acids					323:327	amino acids	317:327	amino acids	317:327	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	6	19	theme	50	1589:1590	arg1	%					1591:1591	%	1591:1591	%	1591:1591	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	2	20	theme	antler	458:463	arg1	slice					465:469	the antler powder and antler slice	436:469	slice	465:469	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	20	theme	antler	458:463	arg1	indicators					424:433	the evaluating indicators	409:433	the evaluating indicators	409:433	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	5	21	theme	acids	1270:1274	arg1	contents					1293:1300	Polysaccharide, crude protein, amino acids, and nucleosides contents	1233:1300	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices"	1233:1319	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	5	22	theme	amino	1264:1268	arg1	acids					1270:1274	amino acids	1264:1274	amino acids	1264:1274	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	2	23	theme	mineral	343:349	arg1	polysaccharides					285:299	polysaccharides	285:299	polysaccharides	285:299	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	23	theme	mineral	343:349	arg1	elements					351:358	mineral elements	343:358	mineral elements	343:358	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	3	24	theme	biogenic	717:724	arg1	"					682:682	fine powder"	671:682	fine powder"	671:682	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	24	theme	biogenic	717:724	arg1	amines					726:731	biogenic amines	717:731	biogenic amines	717:731	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	25	theme	powder	676:681	arg1	"					682:682	fine powder"	671:682	fine powder"	671:682	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	25	theme	powder	676:681	arg1	protein					695:701	the crude protein	685:701	the crude protein	685:701	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	25	theme	powder	676:681	arg1	amines					726:731	biogenic amines	717:731	biogenic amines	717:731	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	6	26	theme	antler	1561:1566	arg1	slice					1568:1572	antler slice	1561:1572	antler slice processed with 50% ethanol	1561:1599	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	1	27	theme	antler	162:167	arg1	"					174:174	"antler slice"	161:174	"antler slice"	161:174	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	3	28	theme	"	682:682	arg1	nucleosides					734:744	fine powder", the crude protein, amino acids, biogenic amines, nucleosides	671:744	fine powder", the crude protein, amino acids, biogenic amines, nucleosides	671:744	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	5	29	theme	nucleosides	1281:1291	arg1	contents					1293:1300	Polysaccharide, crude protein, amino acids, and nucleosides contents	1233:1300	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices"	1233:1319	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	5	30	theme	Polysaccharide	1233:1246	arg1	contents					1293:1300	Polysaccharide, crude protein, amino acids, and nucleosides contents	1233:1300	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices"	1233:1319	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	1	31	theme	slice	169:173	arg1	"					174:174	"antler slice"	161:174	"antler slice"	161:174	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	3	32	theme	nucleosides	897:907	arg1	contents					909:916	nucleosides contents	897:916	nucleosides contents	897:916	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	1	33	theme	"	174:174	arg1	compositions					125:136	the chemical compositions	112:136	the chemical compositions of "antler powder" and "antler slice"	112:174	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	0	34	theme	chemical	13:20	arg1	compositions					22:33	chemical compositions	13:33	chemical compositions in different processed products of Cervi Cornu Pantotrichum	13:93	[Research on chemical compositions in different processed products of Cervi Cornu Pantotrichum].					
29676121	3	35	theme	fine	671:674	arg1	"					682:682	fine powder"	671:682	fine powder"	671:682	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	35	theme	fine	671:674	arg1	protein					695:701	the crude protein	685:701	the crude protein	685:701	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	35	theme	fine	671:674	arg1	amines					726:731	biogenic amines	717:731	biogenic amines	717:731	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	36	theme	antler	1093:1098	arg1	slices					1100:1105	antler slices	1093:1105	antler slices processed with 60% ethanol	1093:1132	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	2	37	theme	biogenic	361:368	arg1	polysaccharides					285:299	polysaccharides	285:299	polysaccharides	285:299	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	37	theme	biogenic	361:368	arg1	amines					370:375	biogenic amines	361:375	biogenic amines	361:375	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	0	38	theme	different	38:46	arg1	products					58:65	different processed products	38:65	different processed products of Cervi Cornu Pantotrichum	38:93	[Research on chemical compositions in different processed products of Cervi Cornu Pantotrichum].					
29676121	2	39	theme	crude	302:306	arg1	polysaccharides					285:299	polysaccharides	285:299	polysaccharides	285:299	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	39	theme	crude	302:306	arg1	protein					308:314	crude protein	302:314	crude protein	302:314	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	40	theme	fatty	330:334	arg1	polysaccharides					285:299	polysaccharides	285:299	polysaccharides	285:299	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	40	theme	fatty	330:334	arg1	acids					336:340	fatty acids	330:340	fatty acids	330:340	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	41	theme	evaluating	413:422	arg1	powder					447:452	the antler powder and antler slice	436:469	powder	447:452	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	41	theme	evaluating	413:422	arg1	slice					465:469	the antler powder and antler slice	436:469	slice	465:469	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	41	theme	evaluating	413:422	arg1	indicators					424:433	the evaluating indicators	409:433	the evaluating indicators	409:433	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	1	42	theme	Chinese	257:263	arg1	Pharmacopoeia					265:277	Chinese Pharmacopoeia	257:277	Chinese Pharmacopoeia	257:277	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	3	43	theme	antler	968:973	arg1	slices					975:980	antler slices	968:980	antler slices processed with 50% ethanol	968:1007	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	44	theme	amino	704:708	arg1	protein					695:701	the crude protein	685:701	the crude protein	685:701	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	44	theme	amino	704:708	arg1	acids					710:714	amino acids	704:714	amino acids	704:714	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	45	theme	antler	831:836	arg1	slices					838:843	antler slices	831:843	antler slices processed with 40% ethanol	831:870	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	4	46	theme	elements	1181:1188	arg1	contents					1145:1152	the contents	1141:1152	the contents of fatty acids and mineral elements	1141:1188	While the contents of fatty acids and mineral elements were not decreased in these three methods.					
29676121	3	47	theme	%	999:999	arg1	ethanol					1001:1007	50% ethanol	997:1007	50% ethanol	997:1007	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	48	theme	crude	1018:1022	arg1	protein					1024:1030	the crude protein and nucleosides contents	1014:1055	protein	1024:1030	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	6	49	theme	%	1591:1591	arg1	ethanol					1593:1599	50% ethanol	1589:1599	50% ethanol	1589:1599	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	0	50	theme	processed	48:56	arg1	products					58:65	different processed products	38:65	different processed products of Cervi Cornu Pantotrichum	38:93	[Research on chemical compositions in different processed products of Cervi Cornu Pantotrichum].					
29676121	5	51	from	contents	1293:1300	arg1	"					1319:1319	"antler slices"	1305:1319	"antler slices"	1305:1319	Polysaccharide, crude protein, amino acids, and nucleosides contents in "antler slices" were less than those in "antler powder", most notably in polysaccharides and nucleosides.					
29676121	4	52	theme	fatty	1157:1161	arg1	acids					1163:1167	fatty acids	1157:1167	fatty acids	1157:1167	While the contents of fatty acids and mineral elements were not decreased in these three methods.					
29676121	6	53	theme	analysis	1472:1479	arg1	scores					1442:1447	the comprehensive scores	1424:1447	the comprehensive scores of principal component analysis (PCA)	1424:1485	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	1	54	theme	chemical	116:123	arg1	compositions					125:136	the chemical compositions	112:136	the chemical compositions of "antler powder" and "antler slice"	112:174	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	3	55	theme	crude	689:693	arg1	"					682:682	fine powder"	671:682	fine powder"	671:682	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	55	theme	crude	689:693	arg1	protein					695:701	the crude protein	685:701	the crude protein	685:701	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	3	55	theme	crude	689:693	arg1	acids					710:714	amino acids	704:714	amino acids	704:714	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	6	56	theme	component	1462:1470	arg1	PCA					1482:1484	PCA	1482:1484	PCA	1482:1484	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	6	56	theme	component	1462:1470	arg1	analysis					1472:1479	principal component analysis	1452:1479	principal component analysis (PCA)	1452:1485	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	3	57	theme	%	862:862	arg1	ethanol					864:870	40% ethanol	860:870	40% ethanol	860:870	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	6	58	theme	comprehensive	1428:1440	arg1	scores					1442:1447	the comprehensive scores	1424:1447	the comprehensive scores of principal component analysis (PCA)	1424:1485	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	2	59	theme	Chinese	508:514	arg1	Pharmacopoeia					516:528	Chinese Pharmacopoeia	508:528	Chinese Pharmacopoeia	508:528	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	3	60	theme	antler	604:609	arg1	powder					611:616	the antler powder	600:616	the antler powder	600:616	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	1	61	theme	processed	190:198	arg1	products					200:207	processed products	190:207	processed products of Cervi Cornu Pantotrichum (CCP)	190:241	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	0	62	theme	Cervi	70:74	arg1	Pantotrichum					82:93	Cervi Cornu Pantotrichum	70:93	Cervi Cornu Pantotrichum	70:93	[Research on chemical compositions in different processed products of Cervi Cornu Pantotrichum].					
29676121	3	63	theme	%	1124:1124	arg1	ethanol					1126:1132	60% ethanol	1122:1132	60% ethanol	1122:1132	The results showed that as compared with the antler powder by directly "chopping into pieces, and grinding into fine powder", the crude protein, amino acids, biogenic amines, nucleosides and nucleobases contents were reduced by 5.01%, 4.35%, 5.90%, 27.62% respectively in antler slices processed with 40% ethanol; the polysaccharides and nucleosides contents were reduced by 24.53% and 21.07% respectively in antler slices processed with 50% ethanol; and the crude protein and nucleosides contents were reduced by 1.65% and 20.52% in antler slices processed with 60% ethanol.					
29676121	6	64	theme	active	1504:1509	arg1	ingredient					1511:1520	active ingredient	1504:1520	active ingredient	1504:1520	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	1	65	theme	products	200:207	arg1	types					181:185	two types	177:185	two types of processed products of Cervi Cornu Pantotrichum (CCP)	177:241	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	2	66	theme	antler	440:445	arg1	powder					447:452	the antler powder and antler slice	436:469	powder	447:452	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	2	66	theme	antler	440:445	arg1	indicators					424:433	the evaluating indicators	409:433	the evaluating indicators	409:433	With polysaccharides, crude protein, amino acids, fatty acids, mineral elements, biogenic amines, nucleosides and nucleobases as the evaluating indicators, the antler powder and antler slice processed with methods documented in Chinese Pharmacopoeia were compared in this study.					
29676121	0	67	from	compositions	22:33	arg1	products					58:65	different processed products	38:65	different processed products of Cervi Cornu Pantotrichum	38:93	[Research on chemical compositions in different processed products of Cervi Cornu Pantotrichum].					
29676121	6	68	theme	ingredient	1511:1520	arg1	lowest					1551:1556	lowest	1551:1556	lowest	1551:1556	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	6	68	theme	ingredient	1511:1520	arg1	decrease					1492:1499	the decrease	1488:1499	the decrease of active ingredient determined in this study	1488:1545	According to the comprehensive scores of principal component analysis (PCA), the decrease of active ingredient determined in this study was lowest in antler slice processed with 50% ethanol.					
29676121	1	69	theme	antler	142:147	arg1	"					155:155	"antler powder"	141:155	"antler powder"	141:155	To investigate the chemical compositions of "antler powder" and "antler slice", two types of processed products of Cervi Cornu Pantotrichum (CCP) documented in Chinese Pharmacopoeia.					
29676121	4	70	theme	mineral	1173:1179	arg1	elements					1181:1188	mineral elements	1173:1188	mineral elements	1173:1188	While the contents of fatty acids and mineral elements were not decreased in these three methods.					
29760197	8	0	theme	same	1485:1488	arg1	function					1502:1509	the same biochemical function	1481:1509	the same biochemical function	1481:1509	This study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function.					
29760197	1	1	theme	ceramide	199:206	arg1	core					208:211	a ceramide core	197:211	a ceramide core linked to a glycan headgroup of varying structures	197:262	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	7	2	from	function	1330:1337	arg1	headgroup					1371:1379	the GIPC headgroup	1362:1379	the GIPC headgroup	1362:1379	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	0	3	theme	Phosphorylceramide	100:117	arg1	TRANSFERASE1					39:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1	0:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1)	0:58	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	0	3	theme	Phosphorylceramide	100:117	arg1	Glycosyltransferase					119:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	8	4	theme	GIPC	1413:1416	arg1	enzyme					1431:1436	a monocot GIPC biosynthetic enzyme	1403:1436	a monocot GIPC biosynthetic enzyme	1403:1436	This study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function.					
29760197	2	5	theme	GIPC	380:383	arg1	glycosylation					385:397	GIPC glycosylation	380:397	GIPC glycosylation	380:397	Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability.					
29760197	6	6	from	lethal	1203:1208	arg1	rice					1088:1091	rice	1088:1091	rice	1088:1091	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	8	7	theme	monocot	1405:1411	arg1	enzyme					1431:1436	a monocot GIPC biosynthetic enzyme	1403:1436	a monocot GIPC biosynthetic enzyme	1403:1436	This study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function.					
29760197	6	8	theme	containing	1109:1118	arg1	subgroup					1145:1152	the major GIPC subgroup	1130:1152	the major GIPC subgroup in vegetative tissue	1130:1173	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	6	8	theme	containing	1109:1118	arg1	GIPCs					1120:1124	GlcN(Ac) containing GIPCs	1100:1124	GlcN(Ac) containing GIPCs	1100:1124	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	8	9	theme	Arabidopsis	1457:1467	arg1	homolog					1469:1475	its Arabidopsis homolog	1453:1475	its Arabidopsis homolog	1453:1475	This study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function.					
29760197	5	10	theme	vegetative	968:977	arg1	growth					979:984	vegetative growth	968:984	vegetative growth	968:984	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	3	11	theme	structural	575:584	arg1	variation					586:594	their structural variation	569:594	their structural variation	569:594	However, the function of GIPCs and the significance of their structural variation are poorly understood.					
29760197	5	12	from	sensitive	1022:1030	arg1	plants					1068:1073	wild-type plants	1058:1073	wild-type plants	1058:1073	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	1	13	theme	plant	300:304	arg1	membrane					313:320	the plant plasma membrane	296:320	the plant plasma membrane	296:320	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	5	14	from	plants	1068:1073	arg1	sensitive					1022:1030	sensitive	1022:1030	sensitive	1022:1030	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	7	15	theme	GIPC	1366:1369	arg1	headgroup					1371:1379	the GIPC headgroup	1362:1379	the GIPC headgroup	1362:1379	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	9	16	theme	cell-cell	1570:1578	arg1	adhesion					1580:1587	cell-cell adhesion	1570:1587	cell-cell adhesion	1570:1587	We also identify a possible role for GIPCs in maintaining cell-cell adhesion.					
29760197	2	17	from	genes	370:374	arg1	thaliana					427:434	Arabidopsis (Arabidopsis thaliana)	402:435	Arabidopsis (Arabidopsis thaliana)	402:435	Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability.					
29760197	4	18	gly	glycosylation	780:792	arg1	subgroup					799:806	a subgroup	797:806	a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)]	797:894	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	5	19	theme	Arabidopsis	900:910	arg1	plants					918:923	Arabidopsis gint1 plants	900:923	Arabidopsis gint1 plants	900:923	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	4	20	dep	GlcNAc	856:861	arg1	Ac					891:892	Ac	891:892	Ac	891:892	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	20	dep	GlcNAc	856:861	arg1	GlcN					886:889	[collectively GlcN	872:889	[collectively GlcN(Ac)	872:893	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	7	21	theme	sugars	1352:1357	arg1	function					1330:1337	the function	1326:1337	the function of different sugars in the GIPC headgroup	1326:1379	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	6	22	theme	major	1134:1138	arg1	subgroup					1145:1152	the major GIPC subgroup	1130:1152	the major GIPC subgroup in vegetative tissue	1130:1173	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	6	22	theme	major	1134:1138	arg1	GIPCs					1120:1124	GlcN(Ac) containing GIPCs	1100:1124	GlcN(Ac) containing GIPCs	1100:1124	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	7	23	from	GIPCs	1272:1276	arg1	Arabidopsis					1281:1291	Arabidopsis leaves	1281:1298	Arabidopsis leaves	1281:1298	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	0	24	theme	INOSITOLPHOSPHORYLCERAMIDE	12:37	arg1	TRANSFERASE1					39:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1	0:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1)	0:58	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	0	24	theme	INOSITOLPHOSPHORYLCERAMIDE	12:37	arg1	Glycosyltransferase					119:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	0	24	theme	INOSITOLPHOSPHORYLCERAMIDE	12:37	arg1	GINT1					53:57	GINT1	53:57	GINT1	53:57	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	1	25	contain	have	192:195	arg2	core					208:211	a ceramide core	197:211	a ceramide core linked to a glycan headgroup of varying structures	197:262	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	1	25	contain	have	192:195	arg1	GIPCs					178:182	GIPCs	178:182	GIPCs	178:182	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	1	25	contain	have	192:195	arg1	phosphorylceramides					157:175	Glycosylinositol phosphorylceramides	140:175	Glycosylinositol phosphorylceramides (GIPCs)	140:183	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	1	25	contain	have	192:195	arg1	sphingolipids					279:291	the major sphingolipids	269:291	the major sphingolipids in the plant plasma membrane	269:320	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	4	26	located	found	817:821	arg1	seeds					826:830	seeds	826:830	seeds	826:830	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	26	located	found	817:821	arg1	pollen					836:841	pollen	836:841	pollen	836:841	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	26	located	found	817:821	arg2	GIPCs					811:815	GIPCs	811:815	GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)]	811:894	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	27	theme	subgroup	799:806	arg1	glycosylation					780:792	the glycosylation	776:792	the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)]	776:894	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	6	28	from	subgroup	1145:1152	arg1	tissue					1168:1173	vegetative tissue	1157:1173	vegetative tissue	1157:1173	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	2	29	theme	Arabidopsis	415:425	arg1	thaliana					427:434	Arabidopsis (Arabidopsis thaliana)	402:435	Arabidopsis (Arabidopsis thaliana)	402:435	Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability.					
29760197	0	30	theme	GLUCOSAMINE	0:10	arg1	TRANSFERASE1					39:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1	0:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1)	0:58	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	0	30	theme	GLUCOSAMINE	0:10	arg1	Glycosyltransferase					119:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	0	30	theme	GLUCOSAMINE	0:10	arg1	GINT1					53:57	GINT1	53:57	GINT1	53:57	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	1	31	theme	plasma	306:311	arg1	membrane					313:320	the plant plasma membrane	296:320	the plant plasma membrane	296:320	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	1	32	theme	Glycosylinositol	140:155	arg1	GIPCs					178:182	GIPCs	178:182	GIPCs	178:182	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	1	32	theme	Glycosylinositol	140:155	arg1	phosphorylceramides					157:175	Glycosylinositol phosphorylceramides	140:175	Glycosylinositol phosphorylceramides (GIPCs)	140:183	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	1	32	theme	Glycosylinositol	140:155	arg1	sphingolipids					279:291	the major sphingolipids	269:291	the major sphingolipids in the plant plasma membrane	269:320	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	7	33	theme	different	1342:1350	arg1	sugars					1352:1357	different sugars	1342:1357	different sugars	1342:1357	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	1	34	theme	glycan	225:230	arg1	headgroup					232:240	a glycan headgroup	223:240	a glycan headgroup of varying structures	223:262	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	9	35	theme	possible	1531:1538	arg1	role					1540:1543	a possible role	1529:1543	a possible role for GIPCs in maintaining cell-cell adhesion	1529:1587	We also identify a possible role for GIPCs in maintaining cell-cell adhesion.					
29760197	5	36	theme	Ac	943:944	arg1	GIPCs					947:951	the GlcN(Ac) GIPCs	934:951	the GlcN(Ac) GIPCs	934:951	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	3	37	theme	variation	586:594	arg1	significance					553:564	the significance	549:564	the significance of their structural variation	549:594	However, the function of GIPCs and the significance of their structural variation are poorly understood.					
29760197	3	37	theme	variation	586:594	arg1	function					527:534	the function	523:534	the function of GIPCs	523:543	However, the function of GIPCs and the significance of their structural variation are poorly understood.					
29760197	4	38	theme	INOSITOLPHOSPHORYLCERAMIDE	690:715	arg1	TRANSFERASE1					717:728	the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1	642:728	the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1)	642:736	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	38	theme	INOSITOLPHOSPHORYLCERAMIDE	690:715	arg1	GINT1					731:735	GINT1	731:735	GINT1	731:735	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	2	39	theme	glycan	463:468	arg1	essential					483:491	essential	483:491	essential	483:491	Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability.					
29760197	2	39	theme	glycan	463:468	arg1	headgroup					470:478	the glycan headgroup	459:478	the glycan headgroup	459:478	Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability.					
29760197	8	40	theme	biosynthetic	1418:1429	arg1	enzyme					1431:1436	a monocot GIPC biosynthetic enzyme	1403:1436	a monocot GIPC biosynthetic enzyme	1403:1436	This study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function.					
29760197	5	41	theme	GIPCs	947:951	arg1	loss					926:929	loss	926:929	loss of the GlcN(Ac) GIPCs	926:951	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	3	42	theme	GIPCs	539:543	arg1	significance					553:564	the significance	549:564	the significance of their structural variation	549:594	However, the function of GIPCs and the significance of their structural variation are poorly understood.					
29760197	3	42	theme	GIPCs	539:543	arg1	function					527:534	the function	523:534	the function of GIPCs	523:543	However, the function of GIPCs and the significance of their structural variation are poorly understood.					
29760197	4	43	theme	GLUCOSAMINE	678:688	arg1	TRANSFERASE1					717:728	the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1	642:728	the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1)	642:736	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	43	theme	GLUCOSAMINE	678:688	arg1	GINT1					731:735	GINT1	731:735	GINT1	731:735	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	6	44	theme	Ac	1105:1106	arg1	subgroup					1145:1152	the major GIPC subgroup	1130:1152	the major GIPC subgroup in vegetative tissue	1130:1173	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	6	44	theme	Ac	1105:1106	arg1	GIPCs					1120:1124	GlcN(Ac) containing GIPCs	1100:1124	GlcN(Ac) containing GIPCs	1100:1124	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	1	45	from	sphingolipids	279:291	arg1	membrane					313:320	the plant plasma membrane	296:320	the plant plasma membrane	296:320	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	1	46	attach	linked	213:218	arg2	core					208:211	a ceramide core	197:211	a ceramide core linked to a glycan headgroup of varying structures	197:262	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	1	46	attach	linked	213:218	arg1	headgroup					232:240	a glycan headgroup	223:240	a glycan headgroup of varying structures	223:262	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	5	47	theme	gint1	912:916	arg1	plants					918:923	Arabidopsis gint1 plants	900:923	Arabidopsis gint1 plants	900:923	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	1	48	theme	varying	245:251	arg1	structures					253:262	varying structures	245:262	varying structures	245:262	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	7	49	theme	GlcN	1263:1266	arg1	GIPCs					1272:1276	"rice-like" GlcN(Ac) GIPCs	1251:1276	"rice-like" GlcN(Ac) GIPCs	1251:1276	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	1	50	theme	structures	253:262	arg1	headgroup					232:240	a glycan headgroup	223:240	a glycan headgroup of varying structures	223:262	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	2	51	theme	plant	497:501	arg1	viability					503:511	plant viability	497:511	plant viability	497:511	Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability.					
29760197	6	52	theme	GINT1	1184:1188	arg1	loss					1176:1179	loss	1176:1179	loss of GINT1	1176:1188	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	7	53	dep	Arabidopsis	1281:1291	arg1	leaves					1293:1298	leaves	1293:1298	leaves	1293:1298	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	8	54	theme	biochemical	1490:1500	arg1	function					1502:1509	the same biochemical function	1481:1509	the same biochemical function	1481:1509	This study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function.					
29760197	7	55	theme	"	1261:1261	arg1	GIPCs					1272:1276	"rice-like" GlcN(Ac) GIPCs	1251:1276	"rice-like" GlcN(Ac) GIPCs	1251:1276	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	5	56	theme	abiotic	1035:1041	arg1	stress					1043:1048	abiotic stress	1035:1048	abiotic stress	1035:1048	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	4	57	theme	[collectively	872:884	arg1	Ac					891:892	Ac	891:892	Ac	891:892	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	57	theme	[collectively	872:884	arg1	GlcN					886:889	[collectively GlcN	872:889	[collectively GlcN(Ac)	872:893	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	8	58	contain	has	1477:1479	arg2	function					1502:1509	the same biochemical function	1481:1509	the same biochemical function	1481:1509	This study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function.					
29760197	8	58	contain	has	1477:1479	arg1	homolog					1469:1475	its Arabidopsis homolog	1453:1475	its Arabidopsis homolog	1453:1475	This study describes a monocot GIPC biosynthetic enzyme and shows that its Arabidopsis homolog has the same biochemical function.					
29760197	5	59	theme	GlcN	938:941	arg1	GIPCs					947:951	the GlcN(Ac) GIPCs	934:951	the GlcN(Ac) GIPCs	934:951	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	6	60	theme	vegetative	1157:1166	arg1	tissue					1168:1173	vegetative tissue	1157:1173	vegetative tissue	1157:1173	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	2	61	theme	biosynthetic	357:368	arg1	genes					370:374	the major biosynthetic genes	347:374	the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana)	347:435	Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability.					
29760197	4	62	theme	GIPCs	811:815	arg1	subgroup					799:806	a subgroup	797:806	a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)]	797:894	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	63	contain	contain	848:854	arg1	seeds					826:830	seeds	826:830	seeds	826:830	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	63	contain	contain	848:854	arg1	pollen					836:841	pollen	836:841	pollen	836:841	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	63	contain	contain	848:854	arg2	GlcNAc					856:861	GlcNAc	856:861	GlcNAc	856:861	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	63	contain	contain	848:854	arg2	GlcN					867:870	GlcN	867:870	GlcN	867:870	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	6	64	theme	GlcN	1100:1103	arg1	subgroup					1145:1152	the major GIPC subgroup	1130:1152	the major GIPC subgroup in vegetative tissue	1130:1173	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	6	64	theme	GlcN	1100:1103	arg1	GIPCs					1120:1124	GlcN(Ac) containing GIPCs	1100:1124	GlcN(Ac) containing GIPCs	1100:1124	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	5	65	theme	seed	996:999	arg1	germination					1001:1011	seed germination	996:1011	seed germination	996:1011	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	6	66	from	rice	1088:1091	arg1	lethal					1203:1208	lethal	1203:1208	lethal	1203:1208	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	2	67	theme	major	351:355	arg1	genes					370:374	the major biosynthetic genes	347:374	the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana)	347:435	Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability.					
29760197	7	68	theme	rice-like	1252:1260	arg1	GIPCs					1272:1276	"rice-like" GlcN(Ac) GIPCs	1251:1276	"rice-like" GlcN(Ac) GIPCs	1251:1276	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	0	69	theme	Glycosylinositol	83:98	arg1	TRANSFERASE1					39:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1	0:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1)	0:58	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	0	69	theme	Glycosylinositol	83:98	arg1	Glycosyltransferase					119:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	5	70	theme	wild-type	1058:1066	arg1	plants					1068:1073	wild-type plants	1058:1073	wild-type plants	1058:1073	In Arabidopsis gint1 plants, loss of the GlcN(Ac) GIPCs did not affect vegetative growth, although seed germination was less sensitive to abiotic stress than in wild-type plants.					
29760197	7	71	theme	Ac	1268:1269	arg1	GIPCs					1272:1276	"rice-like" GlcN(Ac) GIPCs	1251:1276	"rice-like" GlcN(Ac) GIPCs	1251:1276	Furthermore, we could produce, de novo, "rice-like" GlcN(Ac) GIPCs in Arabidopsis leaves, which allowed us to test the function of different sugars in the GIPC headgroup.					
29760197	2	72	theme	Arabidopsis	402:412	arg1	thaliana					427:434	Arabidopsis (Arabidopsis thaliana)	402:435	Arabidopsis (Arabidopsis thaliana)	402:435	Recently, we identified the major biosynthetic genes for GIPC glycosylation in Arabidopsis (Arabidopsis thaliana) and demonstrated that the glycan headgroup is essential for plant viability.					
29760197	6	73	theme	GIPC	1140:1143	arg1	subgroup					1145:1152	the major GIPC subgroup	1130:1152	the major GIPC subgroup in vegetative tissue	1130:1173	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	6	73	theme	GIPC	1140:1143	arg1	GIPCs					1120:1124	GlcN(Ac) containing GIPCs	1100:1124	GlcN(Ac) containing GIPCs	1100:1124	However, in rice, where GlcN(Ac) containing GIPCs are the major GIPC subgroup in vegetative tissue, loss of GINT1 was seedling lethal.					
29760197	4	74	theme	glycosyltransferase	658:676	arg1	TRANSFERASE1					717:728	the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1	642:728	the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1)	642:736	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	74	theme	glycosyltransferase	658:676	arg1	GINT1					731:735	GINT1	731:735	GINT1	731:735	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	0	75	theme	GlcNAc-Containing	65:81	arg1	TRANSFERASE1					39:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1	0:50	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1)	0:58	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	0	75	theme	GlcNAc-Containing	65:81	arg1	Glycosyltransferase					119:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase	63:137	GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) Is a GlcNAc-Containing Glycosylinositol Phosphorylceramide Glycosyltransferase.					
29760197	4	76	theme	Arabidopsis	646:656	arg1	TRANSFERASE1					717:728	the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1	642:728	the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1)	642:736	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	4	76	theme	Arabidopsis	646:656	arg1	GINT1					731:735	GINT1	731:735	GINT1	731:735	Here, we characterized the Arabidopsis glycosyltransferase GLUCOSAMINE INOSITOLPHOSPHORYLCERAMIDE TRANSFERASE1 (GINT1) and showed that it is responsible for the glycosylation of a subgroup of GIPCs found in seeds and pollen that contain GlcNAc and GlcN [collectively GlcN(Ac)].					
29760197	1	77	theme	major	273:277	arg1	phosphorylceramides					157:175	Glycosylinositol phosphorylceramides	140:175	Glycosylinositol phosphorylceramides (GIPCs)	140:183	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29760197	1	77	theme	major	273:277	arg1	sphingolipids					279:291	the major sphingolipids	269:291	the major sphingolipids in the plant plasma membrane	269:320	Glycosylinositol phosphorylceramides (GIPCs), which have a ceramide core linked to a glycan headgroup of varying structures, are the major sphingolipids in the plant plasma membrane.					
29909031	0	0	theme	chitosan/perlite	72:87	arg1	composite					89:97	immobilized cross-linked chitosan/perlite composite	47:97	immobilized cross-linked chitosan/perlite composite	47:97	Synthesis and characterization of Zr(IV) doped immobilized cross-linked chitosan/perlite composite for acid orange II adsorption.					
29909031	5	1	theme	reusable	1027:1034	arg1	adsorption					1061:1070	a reusable, cost-effective and high adsorption	1025:1070	a reusable, cost-effective and high adsorption capacity adsorbent	1025:1089	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	5	1	theme	reusable	1027:1034	arg1	-CS-PT					1015:1020	the Zr(IV)-CS-PT	1005:1020	the Zr(IV)-CS-PT	1005:1020	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	2	2	from	parameters	420:429	arg1	adsorption					439:448	AOII adsorption	434:448	AOII adsorption	434:448	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	1	3	theme	adsorption	145:154	arg1	capacity					156:163	the adsorption capacity	141:163	the adsorption capacity of chitosan for acid orange II (AOII) adsorption	141:212	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	1	4	theme	novel	217:221	arg1	zirconium					234:242	a novel adsorbent, zirconium(IV)	215:246	zirconium	234:242	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	0	5	theme	cross-linked	59:70	arg1	composite					89:97	immobilized cross-linked chitosan/perlite composite	47:97	immobilized cross-linked chitosan/perlite composite	47:97	Synthesis and characterization of Zr(IV) doped immobilized cross-linked chitosan/perlite composite for acid orange II adsorption.					
29909031	2	6	from	effect	400:405	arg1	adsorption					439:448	AOII adsorption	434:448	AOII adsorption	434:448	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	1	7	theme	adsorbent	223:231	arg1	zirconium					234:242	a novel adsorbent, zirconium(IV)	215:246	zirconium	234:242	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	3	8	theme	kinetic	612:618	arg1	model					620:624	pseudo-second order kinetic model	592:624	pseudo-second order kinetic model	592:624	Also, kinetic data revealed that AOII adsorption was well described by pseudo-second order kinetic model.					
29909031	4	9	dep	476.2 mg/g	741:750	arg1	showed					790:795	showed	790:795	showed that the AOII adsorption occurred spontaneously	790:843	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	4	10	theme	AOII	806:809	arg1	adsorption					811:820	the AOII adsorption	802:820	the AOII adsorption	802:820	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	4	11	theme	isotherm	685:692	arg1	data					694:697	the isotherm data	681:697	the isotherm data	681:697	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	5	12	dep	%	990:990	arg1	to					983:984	to	983:984	to	983:984	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	2	13	theme	Zr	459:460	arg1	amount					475:480	Zr (IV) loading amount	459:480	Zr (IV) loading amount	459:480	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	2	14	dep	amount	475:480	arg1	IV					463:464	IV	463:464	IV	463:464	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	4	15	theme	maximum	703:709	arg1	capacity					722:729	maximum adsorption capacity	703:729	maximum adsorption capacity	703:729	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	0	16	theme	acid	103:106	arg1	adsorption					118:127	acid orange II adsorption	103:127	acid orange II adsorption	103:127	Synthesis and characterization of Zr(IV) doped immobilized cross-linked chitosan/perlite composite for acid orange II adsorption.					
29909031	1	17	theme	chitosan	168:175	arg1	capacity					156:163	the adsorption capacity	141:163	the adsorption capacity of chitosan for acid orange II (AOII) adsorption	141:212	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	2	18	theme	AOII	434:437	arg1	adsorption					439:448	AOII adsorption	434:448	AOII adsorption	434:448	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	5	19	theme	recycle	884:890	arg1	experiments					892:902	Desorption and recycle experiments	869:902	experiments	892:902	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	3	20	theme	order	606:610	arg1	model					620:624	pseudo-second order kinetic model	592:624	pseudo-second order kinetic model	592:624	Also, kinetic data revealed that AOII adsorption was well described by pseudo-second order kinetic model.					
29909031	5	21	theme	adsorbent	1081:1089	arg1	adsorption					1061:1070	a reusable, cost-effective and high adsorption	1025:1070	a reusable, cost-effective and high adsorption capacity adsorbent	1025:1089	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	5	21	theme	adsorbent	1081:1089	arg1	-CS-PT					1015:1020	the Zr(IV)-CS-PT	1005:1020	the Zr(IV)-CS-PT	1005:1020	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	4	22	theme	thermodynamic	771:783	arg1	data					785:788	The thermodynamic data	767:788	The thermodynamic data	767:788	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	1	23	theme	acid	181:184	arg1	adsorption					203:212	acid orange II (AOII) adsorption	181:212	acid orange II (AOII) adsorption	181:212	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	1	24	theme	orange	186:191	arg1	adsorption					203:212	acid orange II (AOII) adsorption	181:212	acid orange II (AOII) adsorption	181:212	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	2	25	from	adsorption	439:448	arg1	effect					400:405	the effect	396:405	the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature	396:518	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	2	26	theme	loading	467:473	arg1	amount					475:480	Zr (IV) loading amount	459:480	Zr (IV) loading amount	459:480	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	0	27	theme	orange	108:113	arg1	adsorption					118:127	acid orange II adsorption	103:127	acid orange II adsorption	103:127	Synthesis and characterization of Zr(IV) doped immobilized cross-linked chitosan/perlite composite for acid orange II adsorption.					
29909031	0	28	theme	Zr	34:35	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of Zr(IV) doped immobilized cross-linked chitosan/perlite composite for acid orange II adsorption.					
29909031	0	28	theme	Zr	34:35	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of Zr(IV) doped immobilized cross-linked chitosan/perlite composite for acid orange II adsorption.					
29909031	1	29	theme	immobilized	254:264	arg1	composite					311:319	immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite	254:319	immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite	254:319	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	2	30	theme	parameters	420:429	arg1	effect					400:405	the effect	396:405	the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature	396:518	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	5	31	theme	Zr	1009:1010	arg1	-CS-PT					1015:1020	the Zr(IV)-CS-PT	1005:1020	the Zr(IV)-CS-PT	1005:1020	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	5	31	theme	Zr	1009:1010	arg1	adsorption					1061:1070	a reusable, cost-effective and high adsorption	1025:1070	a reusable, cost-effective and high adsorption capacity adsorbent	1025:1089	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	4	32	from	pH.	763:765	arg1	nature					861:866	endothermic nature	849:866	endothermic nature	849:866	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	4	32	from	pH.	763:765	arg1	476.2 mg/g					741:750	476.2 mg/g	741:750	476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously	741:843	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	1	33	theme	AOII	197:200	arg1	adsorption					203:212	acid orange II (AOII) adsorption	181:212	acid orange II (AOII) adsorption	181:212	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	2	34	theme	different	410:418	arg1	amount					475:480	Zr (IV) loading amount	459:480	Zr (IV) loading amount	459:480	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	2	34	theme	different	410:418	arg1	parameters					420:429	different parameters	410:429	different parameters	410:429	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	2	34	theme	different	410:418	arg1	temperature					508:518	temperature	508:518	temperature	508:518	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	2	34	theme	different	410:418	arg1	pH					483:484	pH	483:484	pH	483:484	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	2	34	theme	different	410:418	arg1	dosage					497:502	adsorbent dosage	487:502	adsorbent dosage	487:502	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	1	35	theme	cross-linked	266:277	arg1	chitosan/perlite					279:294	cross-linked chitosan/perlite	266:294	immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite	254:319	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	1	35	theme	cross-linked	266:277	arg1	-CS-PT					303:308	Zr(IV)-CS-PT	297:308	Zr(IV)-CS-PT	297:308	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	3	36	theme	AOII	554:557	arg1	adsorption					559:568	AOII adsorption	554:568	AOII adsorption	554:568	Also, kinetic data revealed that AOII adsorption was well described by pseudo-second order kinetic model.					
29909031	5	37	theme	%	981:981	arg1	%					990:990	95.6% to 90.1%	977:990	95.6% to 90.1%	977:990	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	0	38	link	cross-linked	59:70	arg1	composite					89:97	immobilized cross-linked chitosan/perlite composite	47:97	immobilized cross-linked chitosan/perlite composite	47:97	Synthesis and characterization of Zr(IV) doped immobilized cross-linked chitosan/perlite composite for acid orange II adsorption.					
29909031	4	39	theme	adsorption	636:645	arg1	model					656:660	Langmuir adsorption isotherm model	627:660	Langmuir adsorption isotherm model	627:660	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	5	40	theme	cost-effective	1037:1050	arg1	adsorption					1061:1070	a reusable, cost-effective and high adsorption	1025:1070	a reusable, cost-effective and high adsorption capacity adsorbent	1025:1089	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	5	40	theme	cost-effective	1037:1050	arg1	-CS-PT					1015:1020	the Zr(IV)-CS-PT	1005:1020	the Zr(IV)-CS-PT	1005:1020	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	1	41	theme	chitosan/perlite	279:294	arg1	composite					311:319	immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite	254:319	immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite	254:319	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	2	42	theme	adsorbent	487:495	arg1	dosage					497:502	adsorbent dosage	487:502	adsorbent dosage	487:502	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	4	43	theme	Langmuir	627:634	arg1	adsorption					636:645	Langmuir adsorption	627:645	Langmuir adsorption isotherm model	627:660	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	4	44	theme	endothermic	849:859	arg1	nature					861:866	endothermic nature	849:866	endothermic nature	849:866	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	3	45	theme	pseudo-second	592:604	arg1	model					620:624	pseudo-second order kinetic model	592:624	pseudo-second order kinetic model	592:624	Also, kinetic data revealed that AOII adsorption was well described by pseudo-second order kinetic model.					
29909031	5	46	theme	adsorption	936:945	arg1	efficiency					947:956	the adsorption efficiency	932:956	the adsorption efficiency	932:956	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	1	47	link	cross-linked	266:277	arg1	chitosan/perlite					279:294	cross-linked chitosan/perlite	266:294	immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite	254:319	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	1	47	link	cross-linked	266:277	arg1	-CS-PT					303:308	Zr(IV)-CS-PT	297:308	Zr(IV)-CS-PT	297:308	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	5	48	theme	Desorption	869:878	arg1	experiments					892:902	Desorption and recycle experiments	869:902	experiments	892:902	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	4	49	theme	adsorption	711:720	arg1	capacity					722:729	maximum adsorption capacity	703:729	maximum adsorption capacity	703:729	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	4	50	theme	isotherm	647:654	arg1	model					656:660	Langmuir adsorption isotherm model	627:660	Langmuir adsorption isotherm model	627:660	Langmuir adsorption isotherm model was best described the isotherm data and maximum adsorption capacity was found 476.2 mg/g at natural pH. The thermodynamic data showed that the AOII adsorption occurred spontaneously and endothermic nature.					
29909031	0	51	theme	immobilized	47:57	arg1	composite					89:97	immobilized cross-linked chitosan/perlite composite	47:97	immobilized cross-linked chitosan/perlite composite	47:97	Synthesis and characterization of Zr(IV) doped immobilized cross-linked chitosan/perlite composite for acid orange II adsorption.					
29909031	2	52	theme	Batch	356:360	arg1	studies					362:368	Batch studies	356:368	Batch studies	356:368	Batch studies were conducted to analyze the effect of different parameters on AOII adsorption, such as Zr (IV) loading amount, pH, adsorbent dosage and temperature.					
29909031	1	53	theme	Zr	297:298	arg1	chitosan/perlite					279:294	cross-linked chitosan/perlite	266:294	immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite	254:319	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	1	53	theme	Zr	297:298	arg1	-CS-PT					303:308	Zr(IV)-CS-PT	297:308	Zr(IV)-CS-PT	297:308	To enhance the adsorption capacity of chitosan for acid orange II (AOII) adsorption, a novel adsorbent, zirconium(IV) doped immobilized cross-linked chitosan/perlite (Zr(IV)-CS-PT) composite was synthesized and characterized.					
29909031	3	54	theme	kinetic	527:533	arg1	data					535:538	kinetic data	527:538	kinetic data	527:538	Also, kinetic data revealed that AOII adsorption was well described by pseudo-second order kinetic model.					
29909031	5	55	theme	high	1056:1059	arg1	adsorption					1061:1070	a reusable, cost-effective and high adsorption	1025:1070	a reusable, cost-effective and high adsorption capacity adsorbent	1025:1089	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29909031	5	55	theme	high	1056:1059	arg1	-CS-PT					1015:1020	the Zr(IV)-CS-PT	1005:1020	the Zr(IV)-CS-PT	1005:1020	Desorption and recycle experiments showed that after six cycle the adsorption efficiency was decreased from 95.6% to 90.1%, which shows the Zr(IV)-CS-PT is a reusable, cost-effective and high adsorption capacity adsorbent.					
29360546	2	0	theme	hydrophobic	564:574	arg1	regions					576:582	biocompatible, hydrophilic and hydrophobic regions	533:582	biocompatible, hydrophilic and hydrophobic regions	533:582	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	1	contain	has	394:396	arg2	electro-activity					482:497	good electro-activity	477:497	good electro-activity	477:497	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	1	contain	has	394:396	arg2	morphology					625:634	nanoturmeric shape morphology	606:634	nanoturmeric shape morphology	606:634	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	1	contain	has	394:396	arg2	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	1	contain	has	394:396	arg1	system					387:392	The developed ternary composite system	355:392	The developed ternary composite system	355:392	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	1	contain	has	394:396	arg2	area					471:474	high surface area	458:474	high surface area	458:474	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	1	contain	has	394:396	arg2	properties					445:454	synergistic improved properties	424:454	synergistic improved properties	424:454	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	1	contain	has	394:396	arg2	conductivity					500:511	conductivity	500:511	conductivity	500:511	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	1	contain	has	394:396	arg2	dispersion					521:530	stable dispersion	514:530	stable dispersion	514:530	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	2	theme	synergistic	424:434	arg1	properties					445:454	synergistic improved properties	424:454	synergistic improved properties	424:454	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	6	3	theme	linear	1061:1066	arg1	range					1068:1072	a linear range	1059:1072	a linear range (0.1 mM-5 mM, R2 = 0.9975)	1059:1099	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	8	4	theme	glucose	1326:1332	arg1	oxidase					1334:1340	glucose oxidase	1326:1340	glucose oxidase	1326:1340	As a model, we incorporate glucose oxidase to detect Glucose.					
29360546	8	4	theme	glucose	1326:1332	arg1	model					1304:1308	a model	1302:1308	a model	1302:1308	As a model, we incorporate glucose oxidase to detect Glucose.					
29360546	5	5	theme	good	953:956	arg1	PCS					920:922	PCS	920:922	PCS	920:922	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	5	5	theme	good	953:956	arg1	material					968:975	a good electrode material	951:975	a good electrode material for sensitive and selective detection of peroxide	951:1025	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	5	5	theme	good	953:956	arg1	HB					928:929	HB	928:929	HB	928:929	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	1	6	theme	good	302:305	arg1	material					317:324	a good electrode material	300:324	a good electrode material for electrochemical sensing	300:352	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	2	7	theme	nanoturmeric	606:617	arg1	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	7	theme	nanoturmeric	606:617	arg1	morphology					625:634	nanoturmeric shape morphology	606:634	nanoturmeric shape morphology	606:634	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	4	8	theme	charge	843:848	arg1	transfer					850:857	the charge transfer	839:857	the charge transfer (potassium ferricyanide used as electroactive marker)	839:911	PCS and HB compatible and effectively communicate with each other, facilitate the charge transfer (potassium ferricyanide used as electroactive marker).					
29360546	1	9	theme	electrode	307:315	arg1	material					317:324	a good electrode material	300:324	a good electrode material for electrochemical sensing	300:352	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	0	10	theme	hydrogen	120:127	arg1	peroxide					129:136	hydrogen peroxide	120:136	hydrogen peroxide	120:136	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material and hemoglobin modified carbon paste electrode for hydrogen peroxide and glucose biosensing.					
29360546	3	11	theme	peroxide	646:653	arg1	biosensor					655:663	Hydrogen peroxide biosensor	637:663	Hydrogen peroxide biosensor	637:663	Hydrogen peroxide biosensor was fabricated as a model system using PCS and hemoglobin (HB) modified carbon paste electrode.					
29360546	3	11	theme	peroxide	646:653	arg1	system					691:696	a model system	683:696	a model system using PCS and hemoglobin (HB) modified carbon paste electrode	683:758	Hydrogen peroxide biosensor was fabricated as a model system using PCS and hemoglobin (HB) modified carbon paste electrode.					
29360546	3	12	theme	Hydrogen	637:644	arg1	peroxide					646:653	Hydrogen peroxide	637:653	Hydrogen peroxide biosensor	637:663	Hydrogen peroxide biosensor was fabricated as a model system using PCS and hemoglobin (HB) modified carbon paste electrode.					
29360546	5	13	theme	sensitive	981:989	arg1	detection					1005:1013	sensitive and selective detection	981:1013	sensitive and selective detection of peroxide	981:1025	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	0	14	theme	glucose	142:148	arg1	biosensing					150:159	glucose biosensing	142:159	glucose biosensing	142:159	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material and hemoglobin modified carbon paste electrode for hydrogen peroxide and glucose biosensing.					
29360546	6	15	theme	long-term	1167:1175	arg1	storage					1177:1183	long-term storage	1167:1183	long-term storage	1167:1183	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	3	16	dep	PCS	704:706	arg1	electrode					750:758	modified carbon paste electrode	728:758	modified carbon paste electrode	728:758	Hydrogen peroxide biosensor was fabricated as a model system using PCS and hemoglobin (HB) modified carbon paste electrode.					
29360546	9	17	theme	low-cost	1448:1455	arg1	strips					1464:1469	low-cost sensor strips	1448:1469	low-cost sensor strips	1448:1469	Thus, the favorable properties indicate that the proposed material system suitable for low-cost sensor strips.					
29360546	2	18	theme	high	458:461	arg1	area					471:474	high surface area	458:474	high surface area	458:474	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	18	theme	high	458:461	arg1	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	1	19	theme	novel	202:206	arg1	nanotubes/starch					260:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch	202:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS)	202:295	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	2	20	theme	multifunctional	585:599	arg1	area					471:474	high surface area	458:474	high surface area	458:474	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	20	theme	multifunctional	585:599	arg1	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	9	21	theme	suitable	1435:1442	arg1	system					1428:1433	the proposed material system	1406:1433	the proposed material system suitable for low-cost sensor strips	1406:1469	Thus, the favorable properties indicate that the proposed material system suitable for low-cost sensor strips.					
29360546	7	22	used	used	1223:1226	arg2	material					1205:1212	This material	1200:1212	This material	1200:1212	This material could be used a platform to develop some other sensors by using other redox enzymes.					
29360546	2	23	theme	stable	514:519	arg1	dispersion					521:530	stable dispersion	514:530	stable dispersion	514:530	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	23	theme	stable	514:519	arg1	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	1	24	theme	nanocomposite	208:220	arg1	nanotubes/starch					260:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch	202:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS)	202:295	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	0	25	theme	carbon	22:27	arg1	material					60:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material	0:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material	0:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material and hemoglobin modified carbon paste electrode for hydrogen peroxide and glucose biosensing.					
29360546	7	26	theme	other	1255:1259	arg1	sensors					1261:1267	some other sensors	1250:1267	some other sensors	1250:1267	This material could be used a platform to develop some other sensors by using other redox enzymes.					
29360546	2	27	theme	composite	377:385	arg1	system					387:392	The developed ternary composite system	355:392	The developed ternary composite system	355:392	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	6	28	dep	range	1068:1072	arg1	R2 = 0.9975					1088:1098	R2 = 0.9975	1088:1098	R2 = 0.9975	1088:1098	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	1	29	theme	material	222:229	arg1	nanotubes/starch					260:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch	202:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS)	202:295	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	0	30	theme	Polyaniline/multiwall	0:20	arg1	material					60:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material	0:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material	0:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material and hemoglobin modified carbon paste electrode for hydrogen peroxide and glucose biosensing.					
29360546	6	31	theme	developed	1032:1040	arg1	biosensor					1042:1050	The developed biosensor	1028:1050	The developed biosensor	1028:1050	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	2	32	theme	ternary	369:375	arg1	system					387:392	The developed ternary composite system	355:392	The developed ternary composite system	355:392	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	1	33	theme	polyaniline/multiwall	231:251	arg1	nanotubes/starch					260:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch	202:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS)	202:295	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	0	34	theme	nanocomposite	46:58	arg1	material					60:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material	0:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material	0:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material and hemoglobin modified carbon paste electrode for hydrogen peroxide and glucose biosensing.					
29360546	1	35	theme	electrochemical	330:344	arg1	sensing					346:352	electrochemical sensing	330:352	electrochemical sensing	330:352	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	2	36	theme	developed	359:367	arg1	system					387:392	The developed ternary composite system	355:392	The developed ternary composite system	355:392	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	1	37	theme	carbon	253:258	arg1	nanotubes/starch					260:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch	202:275	novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS)	202:295	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	0	38	theme	nanotubes/starch	29:44	arg1	material					60:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material	0:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material	0:67	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material and hemoglobin modified carbon paste electrode for hydrogen peroxide and glucose biosensing.					
29360546	5	39	theme	selective	995:1003	arg1	detection					1005:1013	sensitive and selective detection	981:1013	sensitive and selective detection of peroxide	981:1025	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	1	40	theme	nanotubes/starch	260:275	arg1	application					187:197	the application	183:197	the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing	183:352	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	2	41	theme	hydrophilic	548:558	arg1	regions					576:582	biocompatible, hydrophilic and hydrophobic regions	533:582	biocompatible, hydrophilic and hydrophobic regions	533:582	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	5	42	theme	peroxide	1018:1025	arg1	detection					1005:1013	sensitive and selective detection	981:1013	sensitive and selective detection of peroxide	981:1025	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	3	43	theme	modified	728:735	arg1	electrode					750:758	modified carbon paste electrode	728:758	modified carbon paste electrode	728:758	Hydrogen peroxide biosensor was fabricated as a model system using PCS and hemoglobin (HB) modified carbon paste electrode.					
29360546	3	44	theme	model	685:689	arg1	biosensor					655:663	Hydrogen peroxide biosensor	637:663	Hydrogen peroxide biosensor	637:663	Hydrogen peroxide biosensor was fabricated as a model system using PCS and hemoglobin (HB) modified carbon paste electrode.					
29360546	3	44	theme	model	685:689	arg1	system					691:696	a model system	683:696	a model system using PCS and hemoglobin (HB) modified carbon paste electrode	683:758	Hydrogen peroxide biosensor was fabricated as a model system using PCS and hemoglobin (HB) modified carbon paste electrode.					
29360546	2	45	theme	good	477:480	arg1	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	45	theme	good	477:480	arg1	electro-activity					482:497	good electro-activity	477:497	good electro-activity	477:497	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	9	46	theme	sensor	1457:1462	arg1	strips					1464:1469	low-cost sensor strips	1448:1469	low-cost sensor strips	1448:1469	Thus, the favorable properties indicate that the proposed material system suitable for low-cost sensor strips.					
29360546	2	47	theme	shape	619:623	arg1	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	47	theme	shape	619:623	arg1	morphology					625:634	nanoturmeric shape morphology	606:634	nanoturmeric shape morphology	606:634	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	4	48	theme	electroactive	891:903	arg1	marker					905:910	electroactive marker	891:910	electroactive marker	891:910	PCS and HB compatible and effectively communicate with each other, facilitate the charge transfer (potassium ferricyanide used as electroactive marker).					
29360546	5	49	from	PCS	920:922	arg1	combination					935:945	combination	935:945	combination	935:945	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	6	50	theme	detection	1111:1119	arg1	limit					1102:1106	limit	1102:1106	limit of detection (0.032 mM)	1102:1130	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	6	50	theme	detection	1111:1119	arg1	range					1068:1072	a linear range	1059:1072	a linear range (0.1 mM-5 mM, R2 = 0.9975)	1059:1099	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	6	50	theme	detection	1111:1119	arg1	sensitivity					1133:1143	sensitivity	1133:1143	sensitivity (76.43 μA/mM cm2)	1133:1161	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	6	50	theme	detection	1111:1119	arg1	storage					1177:1183	long-term storage	1167:1183	long-term storage	1167:1183	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	6	50	theme	detection	1111:1119	arg1	stability					1189:1197	stability	1189:1197	stability	1189:1197	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	6	50	theme	detection	1111:1119	arg1	76.43 μA/mM cm2					1146:1160	76.43 μA/mM cm2	1146:1160	76.43 μA/mM cm2	1146:1160	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	6	50	theme	detection	1111:1119	arg1	0.032 mM					1122:1129	0.032 mM	1122:1129	0.032 mM	1122:1129	The developed biosensor showed a linear range (0.1 mM-5 mM, R2 = 0.9975), limit of detection (0.032 mM), sensitivity (76.43 μA/mM cm2) and long-term storage and stability.					
29360546	2	51	theme	biocompatible	533:545	arg1	regions					576:582	biocompatible, hydrophilic and hydrophobic regions	533:582	biocompatible, hydrophilic and hydrophobic regions	533:582	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	9	52	theme	favorable	1371:1379	arg1	properties					1381:1390	the favorable properties	1367:1390	the favorable properties	1367:1390	Thus, the favorable properties indicate that the proposed material system suitable for low-cost sensor strips.					
29360546	9	53	dep	indicate	1392:1399	arg1	system					1428:1433	the proposed material system	1406:1433	the proposed material system suitable for low-cost sensor strips	1406:1469	Thus, the favorable properties indicate that the proposed material system suitable for low-cost sensor strips.					
29360546	5	54	theme	electrode	958:966	arg1	PCS					920:922	PCS	920:922	PCS	920:922	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	5	54	theme	electrode	958:966	arg1	material					968:975	a good electrode material	951:975	a good electrode material for sensitive and selective detection of peroxide	951:1025	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	5	54	theme	electrode	958:966	arg1	HB					928:929	HB	928:929	HB	928:929	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	2	55	theme	manifold	398:405	arg1	conductivity					500:511	conductivity	500:511	conductivity	500:511	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	55	theme	manifold	398:405	arg1	area					471:474	high surface area	458:474	high surface area	458:474	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	55	theme	manifold	398:405	arg1	dispersion					521:530	stable dispersion	514:530	stable dispersion	514:530	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	55	theme	manifold	398:405	arg1	electro-activity					482:497	good electro-activity	477:497	good electro-activity	477:497	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	55	theme	manifold	398:405	arg1	morphology					625:634	nanoturmeric shape morphology	606:634	nanoturmeric shape morphology	606:634	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	55	theme	manifold	398:405	arg1	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	7	56	theme	redox	1284:1288	arg1	enzymes					1290:1296	other redox enzymes	1278:1296	other redox enzymes	1278:1296	This material could be used a platform to develop some other sensors by using other redox enzymes.					
29360546	2	57	dep	interactions	407:418	arg1	conductivity					500:511	conductivity	500:511	conductivity	500:511	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	57	dep	interactions	407:418	arg1	area					471:474	high surface area	458:474	high surface area	458:474	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	57	dep	interactions	407:418	arg1	dispersion					521:530	stable dispersion	514:530	stable dispersion	514:530	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	57	dep	interactions	407:418	arg1	regions					576:582	biocompatible, hydrophilic and hydrophobic regions	533:582	biocompatible, hydrophilic and hydrophobic regions	533:582	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	57	dep	interactions	407:418	arg1	electro-activity					482:497	good electro-activity	477:497	good electro-activity	477:497	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	57	dep	interactions	407:418	arg1	morphology					625:634	nanoturmeric shape morphology	606:634	nanoturmeric shape morphology	606:634	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	57	dep	interactions	407:418	arg1	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	3	58	theme	carbon	737:742	arg1	electrode					750:758	modified carbon paste electrode	728:758	modified carbon paste electrode	728:758	Hydrogen peroxide biosensor was fabricated as a model system using PCS and hemoglobin (HB) modified carbon paste electrode.					
29360546	4	59	theme	potassium	860:868	arg1	ferricyanide					870:881	potassium ferricyanide	860:881	potassium ferricyanide used as electroactive marker	860:910	PCS and HB compatible and effectively communicate with each other, facilitate the charge transfer (potassium ferricyanide used as electroactive marker).					
29360546	4	60	dep	transfer	850:857	arg1	ferricyanide					870:881	potassium ferricyanide	860:881	potassium ferricyanide used as electroactive marker	860:910	PCS and HB compatible and effectively communicate with each other, facilitate the charge transfer (potassium ferricyanide used as electroactive marker).					
29360546	4	61	dep	communicate	799:809	arg1	facilitate					828:837	facilitate	828:837	facilitate the charge transfer (potassium ferricyanide used as electroactive marker)	828:911	PCS and HB compatible and effectively communicate with each other, facilitate the charge transfer (potassium ferricyanide used as electroactive marker).					
29360546	0	62	theme	paste	100:104	arg1	electrode					106:114	carbon paste electrode	93:114	carbon paste electrode for hydrogen peroxide and glucose biosensing	93:159	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material and hemoglobin modified carbon paste electrode for hydrogen peroxide and glucose biosensing.					
29360546	3	63	theme	paste	744:748	arg1	electrode					750:758	modified carbon paste electrode	728:758	modified carbon paste electrode	728:758	Hydrogen peroxide biosensor was fabricated as a model system using PCS and hemoglobin (HB) modified carbon paste electrode.					
29360546	7	64	theme	other	1278:1282	arg1	enzymes					1290:1296	other redox enzymes	1278:1296	other redox enzymes	1278:1296	This material could be used a platform to develop some other sensors by using other redox enzymes.					
29360546	2	65	theme	surface	463:469	arg1	area					471:474	high surface area	458:474	high surface area	458:474	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	2	65	theme	surface	463:469	arg1	interactions					407:418	manifold interactions	398:418	manifold interactions	398:418	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	9	66	theme	proposed	1410:1417	arg1	system					1428:1433	the proposed material system	1406:1433	the proposed material system suitable for low-cost sensor strips	1406:1469	Thus, the favorable properties indicate that the proposed material system suitable for low-cost sensor strips.					
29360546	0	67	theme	carbon	93:98	arg1	electrode					106:114	carbon paste electrode	93:114	carbon paste electrode for hydrogen peroxide and glucose biosensing	93:159	Polyaniline/multiwall carbon nanotubes/starch nanocomposite material and hemoglobin modified carbon paste electrode for hydrogen peroxide and glucose biosensing.					
29360546	1	68	dep	nanotubes/starch	260:275	arg1	designated					278:287	designated	278:287	designated as PCS	278:294	This paper describes the application of novel nanocomposite material polyaniline/multiwall carbon nanotubes/starch (designated as PCS) as a good electrode material for electrochemical sensing.					
29360546	5	69	from	HB	928:929	arg1	combination					935:945	combination	935:945	combination	935:945	Thus, PCS and HB (in combination) is a good electrode material for sensitive and selective detection of peroxide.					
29360546	2	70	theme	improved	436:443	arg1	properties					445:454	synergistic improved properties	424:454	synergistic improved properties	424:454	The developed ternary composite system has manifold interactions and synergistic improved properties - high surface area, good electro-activity, conductivity, stable dispersion, biocompatible, hydrophilic and hydrophobic regions, multifunctional, and nanoturmeric shape morphology.					
29360546	9	71	theme	material	1419:1426	arg1	system					1428:1433	the proposed material system	1406:1433	the proposed material system suitable for low-cost sensor strips	1406:1469	Thus, the favorable properties indicate that the proposed material system suitable for low-cost sensor strips.					
31423772	3	0	theme	GAA	692:694	arg1	Cu-MOF					700:705	GAA@GOx@Cu-MOF	692:705	GAA@GOx@Cu-MOF	692:705	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	3	1	theme	free	900:903	arg1	system					912:917	the free enzyme system	896:917	the free enzyme system	896:917	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	3	2	theme	assembly	815:822	arg1	work					829:832	assembly line work	815:832	assembly line work	815:832	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	2	3	theme	framework	479:487	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	3	theme	framework	479:487	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	1	4	theme	test	348:351	arg1	compounds					353:361	test compounds	348:361	test compounds	348:361	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	2	5	with	reactor	489:495	arg1	structure					544:552	a flower-shaped globular structure	519:552	a flower-shaped globular structure	519:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	6	6	theme	functional	1375:1384	arg1	composites					1390:1399	functional MOF composites	1375:1399	functional MOF composites	1375:1399	Therefore, it was expected that this work could provide new insights and inspirations for the screening of clinical antidiabetic drugs and for further exploration of functional MOF composites.					
31423772	1	7	theme	practical	270:278	arg1	stability					307:315	poor stability	302:315	poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on	302:409	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	7	theme	practical	270:278	arg1	applications					280:291	practical applications	270:291	practical applications	270:291	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	8	theme	compounds	353:361	arg1	enzyme					320:325	enzyme	320:325	enzyme	320:325	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	8	theme	compounds	353:361	arg1	consumption					333:343	high consumption	328:343	high consumption of test compounds	328:361	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	6	9	theme	drugs	1338:1342	arg1	screening					1303:1311	the screening	1299:1311	the screening of clinical antidiabetic drugs	1299:1342	Therefore, it was expected that this work could provide new insights and inspirations for the screening of clinical antidiabetic drugs and for further exploration of functional MOF composites.					
31423772	2	10	theme	oxidase	632:638	arg1	self-assembling					584:598	self-assembling	584:598	self-assembling	584:598	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	5	11	theme	potential	1179:1187	arg1	drugs					1202:1206	potential antidiabetic drugs	1179:1206	potential antidiabetic drugs	1179:1206	Furthermore, the proposed method was successfully applied to the screening of oleanolic acid derivatives as potential antidiabetic drugs.					
31423772	6	12	theme	MOF	1386:1388	arg1	composites					1390:1399	functional MOF composites	1375:1399	functional MOF composites	1375:1399	Therefore, it was expected that this work could provide new insights and inspirations for the screening of clinical antidiabetic drugs and for further exploration of functional MOF composites.					
31423772	2	13	theme	@	505:505	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	13	theme	@	505:505	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	6	14	theme	composites	1390:1399	arg1	exploration					1360:1370	further exploration	1352:1370	further exploration of functional MOF composites	1352:1399	Therefore, it was expected that this work could provide new insights and inspirations for the screening of clinical antidiabetic drugs and for further exploration of functional MOF composites.					
31423772	2	15	theme	glucose	624:630	arg1	oxidase					632:638	glucose oxidase	624:638	glucose oxidase (GOx)	624:644	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	15	theme	glucose	624:630	arg1	GOx					641:643	GOx	641:643	GOx	641:643	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	3	16	theme	selectivity	750:760	arg1	merits					724:729	merits	724:729	merits of high stability, selectivity, and sensitivity	724:777	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	2	17	theme	GOx	502:504	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	17	theme	GOx	502:504	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	1	18	theme	low	364:366	arg1	enzyme					320:325	enzyme	320:325	enzyme	320:325	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	18	theme	low	364:366	arg1	sensitivity					368:378	low sensitivity	364:378	low sensitivity of screening methods and so on	364:409	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	5	19	theme	derivatives	1164:1174	arg1	screening					1136:1144	the screening	1132:1144	the screening of oleanolic acid derivatives as potential antidiabetic drugs	1132:1206	Furthermore, the proposed method was successfully applied to the screening of oleanolic acid derivatives as potential antidiabetic drugs.					
31423772	6	20	theme	antidiabetic	1325:1336	arg1	drugs					1338:1342	clinical antidiabetic drugs	1316:1342	clinical antidiabetic drugs	1316:1342	Therefore, it was expected that this work could provide new insights and inspirations for the screening of clinical antidiabetic drugs and for further exploration of functional MOF composites.					
31423772	2	21	theme	@	501:501	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	21	theme	@	501:501	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	22	theme	organic	471:477	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	22	theme	organic	471:477	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	23	theme	Cu2+	647:650	arg1	self-assembling					584:598	self-assembling	584:598	self-assembling	584:598	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	4	24	theme	above	933:937	arg1	characteristics					939:953	the above characteristics	929:953	the above characteristics	929:953	Based on the above characteristics, a highly sensitive screening of GAA inhibitors could be achieved with the detection limit of 7.05 nM for acarbose.					
31423772	3	25	theme	line	824:827	arg1	work					829:832	assembly line work	815:832	assembly line work	815:832	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	6	26	theme	new	1265:1267	arg1	insights					1269:1276	new insights	1265:1276	new insights	1265:1276	Therefore, it was expected that this work could provide new insights and inspirations for the screening of clinical antidiabetic drugs and for further exploration of functional MOF composites.					
31423772	0	27	theme	Hybrid	13:18	arg1	Reactors					61:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors for α-Glucosidase Inhibitor Screening.					
31423772	3	28	contain	possessed	788:796	arg2	character					802:810	the character	798:810	the character of assembly line work	798:832	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	3	28	contain	possessed	788:796	arg1	Cu-MOF					700:705	GAA@GOx@Cu-MOF	692:705	GAA@GOx@Cu-MOF	692:705	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	2	29	theme	GAA	498:500	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	29	theme	GAA	498:500	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	3	30	theme	work	829:832	arg1	character					802:810	the character	798:810	the character of assembly line work	798:832	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	0	31	theme	Bifunctional	0:11	arg1	Reactors					61:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors for α-Glucosidase Inhibitor Screening.					
31423772	3	32	theme	GOx	696:698	arg1	Cu-MOF					700:705	GAA@GOx@Cu-MOF	692:705	GAA@GOx@Cu-MOF	692:705	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	2	33	theme	α-glucosidase	603:615	arg1	self-assembling					584:598	self-assembling	584:598	self-assembling	584:598	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	1	34	theme	screening	113:121	arg1	strategy					123:130	The screening strategy	109:130	The screening strategy based on α-glucosidase inhibition	109:164	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	35	theme	antidiabetic	212:223	arg1	drugs					225:229	antidiabetic drugs	212:229	antidiabetic drugs	212:229	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	0	36	theme	Metal	37:41	arg1	Reactors					61:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors for α-Glucosidase Inhibitor Screening.					
31423772	3	37	theme	@	699:699	arg1	Cu-MOF					700:705	GAA@GOx@Cu-MOF	692:705	GAA@GOx@Cu-MOF	692:705	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	2	38	theme	flower-shaped	521:533	arg1	structure					544:552	a flower-shaped globular structure	519:552	a flower-shaped globular structure	519:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	1	39	theme	drugs	225:229	arg1	discovery					199:207	the discovery	195:207	the discovery of antidiabetic drugs	195:229	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	0	40	theme	Enzyme-Catalytic	20:35	arg1	Reactors					61:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors for α-Glucosidase Inhibitor Screening.					
31423772	1	41	theme	poor	302:305	arg1	stability					307:315	poor stability	302:315	poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on	302:409	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	42	theme	screening	383:391	arg1	methods					393:399	screening methods	383:399	screening methods	383:399	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	4	43	theme	detection	1030:1038	arg1	limit					1040:1044	the detection limit	1026:1044	the detection limit of 7.05 nM for acarbose	1026:1068	Based on the above characteristics, a highly sensitive screening of GAA inhibitors could be achieved with the detection limit of 7.05 nM for acarbose.					
31423772	0	44	theme	Framework	51:59	arg1	Reactors					61:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors for α-Glucosidase Inhibitor Screening.					
31423772	3	45	theme	@	695:695	arg1	Cu-MOF					700:705	GAA@GOx@Cu-MOF	692:705	GAA@GOx@Cu-MOF	692:705	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	1	46	theme	methods	393:399	arg1	enzyme					320:325	enzyme	320:325	enzyme	320:325	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	46	theme	methods	393:399	arg1	sensitivity					368:378	low sensitivity	364:378	low sensitivity of screening methods and so on	364:409	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	5	47	theme	antidiabetic	1189:1200	arg1	drugs					1202:1206	potential antidiabetic drugs	1179:1206	potential antidiabetic drugs	1179:1206	Furthermore, the proposed method was successfully applied to the screening of oleanolic acid derivatives as potential antidiabetic drugs.					
31423772	0	48	theme	Organic	43:49	arg1	Reactors					61:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors	0:68	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors for α-Glucosidase Inhibitor Screening.					
31423772	2	49	theme	metal	465:469	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	49	theme	metal	465:469	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	0	50	dep	Reactors	61:68	arg1	Screening					98:106	α-Glucosidase Inhibitor Screening	74:106	α-Glucosidase Inhibitor Screening	74:106	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors for α-Glucosidase Inhibitor Screening.					
31423772	2	51	theme	enzyme-catalytic	448:463	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	51	theme	enzyme-catalytic	448:463	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	1	52	theme	enzyme	320:325	arg1	stability					307:315	poor stability	302:315	poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on	302:409	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	3	53	theme	high	734:737	arg1	stability					739:747	high stability	734:747	high stability	734:747	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	2	54	theme	4,4'-bipyridine	657:671	arg1	self-assembling					584:598	self-assembling	584:598	self-assembling	584:598	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	6	55	theme	further	1352:1358	arg1	exploration					1360:1370	further exploration	1352:1370	further exploration of functional MOF composites	1352:1399	Therefore, it was expected that this work could provide new insights and inspirations for the screening of clinical antidiabetic drugs and for further exploration of functional MOF composites.					
31423772	2	56	theme	hybrid	441:446	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	56	theme	hybrid	441:446	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	6	57	theme	clinical	1316:1323	arg1	drugs					1338:1342	clinical antidiabetic drugs	1316:1342	clinical antidiabetic drugs	1316:1342	Therefore, it was expected that this work could provide new insights and inspirations for the screening of clinical antidiabetic drugs and for further exploration of functional MOF composites.					
31423772	1	58	from	challenges	256:265	arg1	stability					307:315	poor stability	302:315	poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on	302:409	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	58	from	challenges	256:265	arg1	applications					280:291	practical applications	270:291	practical applications	270:291	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	59	theme	on	408:409	arg1	enzyme					320:325	enzyme	320:325	enzyme	320:325	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	59	theme	on	408:409	arg1	sensitivity					368:378	low sensitivity	364:378	low sensitivity of screening methods and so on	364:409	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	3	60	theme	enzyme	866:871	arg1	activity					873:880	enzyme activity	866:880	enzyme activity	866:880	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	1	61	theme	α-glucosidase	141:153	arg1	inhibition					155:164	α-glucosidase inhibition	141:164	α-glucosidase inhibition	141:164	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	4	62	theme	inhibitors	992:1001	arg1	screening					975:983	a highly sensitive screening	956:983	a highly sensitive screening of GAA inhibitors	956:1001	Based on the above characteristics, a highly sensitive screening of GAA inhibitors could be achieved with the detection limit of 7.05 nM for acarbose.					
31423772	1	63	theme	high	328:331	arg1	enzyme					320:325	enzyme	320:325	enzyme	320:325	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	1	63	theme	high	328:331	arg1	consumption					333:343	high consumption	328:343	high consumption of test compounds	328:361	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	4	64	theme	GAA	988:990	arg1	inhibitors					992:1001	GAA inhibitors	988:1001	GAA inhibitors	988:1001	Based on the above characteristics, a highly sensitive screening of GAA inhibitors could be achieved with the detection limit of 7.05 nM for acarbose.					
31423772	1	65	dep	and	401:403	arg1	so					405:406	so	405:406	so	405:406	The screening strategy based on α-glucosidase inhibition has been widely employed for the discovery of antidiabetic drugs, but it still faces some challenges in practical applications, such as poor stability of enzyme, high consumption of test compounds, low sensitivity of screening methods and so on.					
31423772	2	66	theme	globular	535:542	arg1	structure					544:552	a flower-shaped globular structure	519:552	a flower-shaped globular structure	519:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	3	67	theme	enzyme	905:910	arg1	system					912:917	the free enzyme system	896:917	the free enzyme system	896:917	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	4	68	theme	nM	1054:1055	arg1	limit					1040:1044	the detection limit	1026:1044	the detection limit of 7.05 nM for acarbose	1026:1068	Based on the above characteristics, a highly sensitive screening of GAA inhibitors could be achieved with the detection limit of 7.05 nM for acarbose.					
31423772	3	69	theme	sensitivity	767:777	arg1	merits					724:729	merits	724:729	merits of high stability, selectivity, and sensitivity	724:777	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	3	70	theme	stability	739:747	arg1	merits					724:729	merits	724:729	merits of high stability, selectivity, and sensitivity	724:777	It was found that GAA@GOx@Cu-MOF not only enjoyed merits of high stability, selectivity, and sensitivity but also possessed the character of assembly line work, with about 4.58 times enhanced enzyme activity compared with the free enzyme system.					
31423772	0	71	theme	Inhibitor	88:96	arg1	Screening					98:106	α-Glucosidase Inhibitor Screening	74:106	α-Glucosidase Inhibitor Screening	74:106	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors for α-Glucosidase Inhibitor Screening.					
31423772	5	72	theme	acid	1159:1162	arg1	derivatives					1164:1174	oleanolic acid derivatives	1149:1174	oleanolic acid derivatives as potential antidiabetic drugs	1149:1206	Furthermore, the proposed method was successfully applied to the screening of oleanolic acid derivatives as potential antidiabetic drugs.					
31423772	0	73	theme	α-Glucosidase	74:86	arg1	Screening					98:106	α-Glucosidase Inhibitor Screening	74:106	α-Glucosidase Inhibitor Screening	74:106	Bifunctional Hybrid Enzyme-Catalytic Metal Organic Framework Reactors for α-Glucosidase Inhibitor Screening.					
31423772	5	74	theme	proposed	1088:1095	arg1	method					1097:1102	the proposed method	1084:1102	the proposed method	1084:1102	Furthermore, the proposed method was successfully applied to the screening of oleanolic acid derivatives as potential antidiabetic drugs.					
31423772	2	75	theme	bifunctional	428:439	arg1	reactor					489:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor	426:495	a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure	426:552	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	2	75	theme	bifunctional	428:439	arg1	Cu-MOF					506:511	GAA@GOx@Cu-MOF	498:511	GAA@GOx@Cu-MOF	498:511	In this work, a bifunctional hybrid enzyme-catalytic metal organic framework reactor (GAA@GOx@Cu-MOF) with a flower-shaped globular structure was innovatively prepared via self-assembling of α-glucosidase (GAA), glucose oxidase (GOx), Cu2+, and 4,4'-bipyridine.					
31423772	4	76	theme	sensitive	965:973	arg1	screening					975:983	a highly sensitive screening	956:983	a highly sensitive screening of GAA inhibitors	956:1001	Based on the above characteristics, a highly sensitive screening of GAA inhibitors could be achieved with the detection limit of 7.05 nM for acarbose.					
31423772	5	77	theme	oleanolic	1149:1157	arg1	derivatives					1164:1174	oleanolic acid derivatives	1149:1174	oleanolic acid derivatives as potential antidiabetic drugs	1149:1206	Furthermore, the proposed method was successfully applied to the screening of oleanolic acid derivatives as potential antidiabetic drugs.					
30322852	6	0	theme	synthesis	1092:1100	arg1	Alteration					1071:1080	Alteration	1071:1080	Alteration of glucan synthesis by both mutants	1071:1116	Alteration of glucan synthesis by both mutants yielded biofilms with less dry weight and insoluble EPS.					
30322852	11	1	theme	optimal	2407:2413	arg1	formation					2423:2431	optimal biofilm formation	2407:2431	optimal biofilm formation in streptococci	2407:2447	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	6	2	with	biofilms	1126:1133	arg1	weight					1149:1154	less dry weight	1140:1154	less dry weight	1140:1154	Alteration of glucan synthesis by both mutants yielded biofilms with less dry weight and insoluble EPS.					
30322852	6	2	with	biofilms	1126:1133	arg1	EPS					1170:1172	insoluble EPS	1160:1172	insoluble EPS	1160:1172	Alteration of glucan synthesis by both mutants yielded biofilms with less dry weight and insoluble EPS.					
30322852	4	3	theme	wild	874:877	arg1	type					879:882	the wild type	870:882	the wild type	870:882	Compared to the wild type, the ΔyidC2 mutant lacked staining with fluorescent vancomycin at the division septum, while the ΔyidC1 mutant resembled the wild type.					
30322852	12	4	theme	EPS	2564:2566	arg1	matrix					2568:2573	the EPS matrix	2560:2573	the EPS matrix produced by S. mutans	2560:2595	Here, we show that the deletion of either yidC results in changes to the structure and physical properties of the EPS matrix produced by S. mutans, ultimately impairing optimal biofilm development, diminishing its mechanical stability, and facilitating its removal.					
30322852	11	5	theme	cariogenic	2248:2257	arg1	mutans					2282:2287	the cariogenic pathogen Streptococcus mutans	2244:2287	the cariogenic pathogen Streptococcus mutans	2244:2287	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	3	6	theme	cell	543:546	arg1	properties					556:565	cell surface properties	543:565	cell surface properties	543:565	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	13	7	theme	universal	2733:2741	arg1	conservation					2743:2754	the universal conservation	2729:2754	the universal conservation	2729:2754	Importantly, the universal conservation of bacterial yidC orthologs, combined with our findings, provide a rationale for YidC as a possible drug target for antibiofilm therapies.					
30322852	3	8	theme	biofilm	714:720	arg1	properties					556:565	cell surface properties	543:565	cell surface properties	543:565	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	3	8	theme	biofilm	714:720	arg1	properties					690:699	biophysical properties	678:699	biophysical properties of S. mutans biofilm	678:720	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	3	9	theme	yidC	530:533	arg1	deletion					518:525	the deletion	514:525	the deletion of yidC	514:533	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	7	10	dep	three-dimensional	1356:1372	arg1	3D					1375:1376	3D	1375:1376	3D	1375:1376	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	4	11	theme	ΔyidC1	846:851	arg1	mutant					853:858	the ΔyidC1 mutant	842:858	the ΔyidC1 mutant	842:858	Compared to the wild type, the ΔyidC2 mutant lacked staining with fluorescent vancomycin at the division septum, while the ΔyidC1 mutant resembled the wild type.					
30322852	11	12	theme	envelope	2313:2320	arg1	biogenesis					2322:2331	proper envelope biogenesis	2306:2331	proper envelope biogenesis	2306:2331	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	3	13	theme	extracellular	568:580	arg1	production					589:598	extracellular glucan production	568:598	extracellular glucan production	568:598	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	1	14	theme	biofilm-forming	180:194	arg1	biogenesis					142:151	Proper envelope biogenesis	126:151	Proper envelope biogenesis of Streptococcus mutans	126:175	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	14	theme	biofilm-forming	180:194	arg1	pathogen					227:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	6	15	theme	dry	1145:1147	arg1	weight					1149:1154	less dry weight	1140:1154	less dry weight	1140:1154	Alteration of glucan synthesis by both mutants yielded biofilms with less dry weight and insoluble EPS.					
30322852	10	16	theme	membrane	2181:2188	arg1	insertion					2198:2206	membrane protein insertion	2181:2206	membrane protein insertion	2181:2206	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	0	17	theme	Biophysical	102:112	arg1	Streptococcus					0:12	Streptococcus	0:12	Streptococcus	0:12	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	0	17	theme	Biophysical	102:112	arg1	Properties					114:123	Biofilm Biophysical Properties	94:123	Biofilm Biophysical Properties	94:123	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	6	18	theme	glucan	1085:1090	arg1	synthesis					1092:1100	glucan synthesis	1085:1100	glucan synthesis	1085:1100	Alteration of glucan synthesis by both mutants yielded biofilms with less dry weight and insoluble EPS.					
30322852	1	19	theme	dental	200:205	arg1	biogenesis					142:151	Proper envelope biogenesis	126:151	Proper envelope biogenesis of Streptococcus mutans	126:175	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	19	theme	dental	200:205	arg1	pathogen					227:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	9	20	theme	paralog	1678:1684	arg1	elimination					1651:1661	the elimination	1647:1661	the elimination of either yidC paralog	1647:1684	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	12	21	theme	matrix	2568:2573	arg1	structure					2523:2531	structure	2523:2531	structure	2523:2531	Here, we show that the deletion of either yidC results in changes to the structure and physical properties of the EPS matrix produced by S. mutans, ultimately impairing optimal biofilm development, diminishing its mechanical stability, and facilitating its removal.					
30322852	12	21	theme	matrix	2568:2573	arg1	properties					2546:2555	physical properties	2537:2555	physical properties	2537:2555	Here, we show that the deletion of either yidC results in changes to the structure and physical properties of the EPS matrix produced by S. mutans, ultimately impairing optimal biofilm development, diminishing its mechanical stability, and facilitating its removal.					
30322852	10	22	theme	biofilm	2001:2007	arg1	proteins					2033:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	10	22	theme	biofilm	2001:2007	arg1	insertases					2075:2084	membrane-localized chaperone insertases	2046:2084	membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly	2046:2219	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	9	23	dep	envelope	1717:1724	arg1	the					1708:1710	the	1708:1710	the	1708:1710	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	1	24	theme	oral	222:225	arg1	biogenesis					142:151	Proper envelope biogenesis	126:151	Proper envelope biogenesis of Streptococcus mutans	126:175	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	24	theme	oral	222:225	arg1	pathogen					227:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	10	25	theme	assembly	2212:2219	arg1	context					2170:2176	the context	2166:2176	the context of membrane protein insertion and assembly	2166:2219	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	9	26	dep	disrupts	1764:1771	arg1	altering					1839:1846	altering	1839:1846	altering the physical properties of the biofilms	1839:1886	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	9	26	dep	disrupts	1764:1771	arg1	facilitating					1892:1903	facilitating	1892:1903	facilitating their removal	1892:1917	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	8	27	theme	high	1464:1467	arg1	density					1474:1480	high cell density	1464:1480	high cell density	1464:1480	The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear.					
30322852	10	28	theme	insertion	2198:2206	arg1	context					2170:2176	the context	2166:2176	the context of membrane protein insertion and assembly	2166:2219	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	13	29	theme	drug	2856:2859	arg1	rationale					2823:2831	a rationale	2821:2831	a rationale for YidC	2821:2840	Importantly, the universal conservation of bacterial yidC orthologs, combined with our findings, provide a rationale for YidC as a possible drug target for antibiofilm therapies.					
30322852	13	29	theme	drug	2856:2859	arg1	target					2861:2866	a possible drug target	2845:2866	a possible drug target for antibiofilm therapies	2845:2892	Importantly, the universal conservation of bacterial yidC orthologs, combined with our findings, provide a rationale for YidC as a possible drug target for antibiofilm therapies.					
30322852	1	30	theme	Proper	126:131	arg1	biogenesis					142:151	Proper envelope biogenesis	126:151	Proper envelope biogenesis of Streptococcus mutans	126:175	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	30	theme	Proper	126:131	arg1	pathogen					227:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	9	31	theme	cell	1712:1715	arg1	envelope					1717:1724	cell envelope	1712:1724	cell envelope	1712:1724	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	7	32	theme	matrix	1329:1334	arg1	organization					1305:1316	the spatial organization	1293:1316	the spatial organization of the EPS matrix	1293:1334	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	10	33	theme	YidC	2028:2031	arg1	proteins					2033:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	10	33	theme	YidC	2028:2031	arg1	insertases					2075:2084	membrane-localized chaperone insertases	2046:2084	membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly	2046:2219	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	3	34	theme	structural	612:621	arg1	organization					623:634	the structural organization	608:634	the structural organization of the exopolysaccharide (EPS) matrix	608:672	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	7	35	theme	yidC2	1206:1210	arg1	deletion					1194:1201	the deletion	1190:1201	the deletion of yidC2	1190:1210	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	1	36	theme	integral	340:347	arg1	chaperones					349:358	membrane integral chaperones	331:358	membrane integral chaperones	331:358	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	37	theme	Streptococcus	156:168	arg1	mutans					170:175	Streptococcus mutans	156:175	Streptococcus mutans	156:175	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	12	38	theme	biofilm	2627:2633	arg1	development					2635:2645	optimal biofilm development	2619:2645	optimal biofilm development	2619:2645	Here, we show that the deletion of either yidC results in changes to the structure and physical properties of the EPS matrix produced by S. mutans, ultimately impairing optimal biofilm development, diminishing its mechanical stability, and facilitating its removal.					
30322852	4	39	theme	ΔyidC2	754:759	arg1	mutant					761:766	the ΔyidC2 mutant	750:766	the ΔyidC2 mutant	750:766	Compared to the wild type, the ΔyidC2 mutant lacked staining with fluorescent vancomycin at the division septum, while the ΔyidC1 mutant resembled the wild type.					
30322852	10	40	theme	chaperone	2065:2073	arg1	proteins					2033:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	10	40	theme	chaperone	2065:2073	arg1	insertases					2075:2084	membrane-localized chaperone insertases	2046:2084	membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly	2046:2219	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	11	41	theme	mutans	2282:2287	arg1	paralogs					2232:2239	Both YidC paralogs	2222:2239	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans	2222:2287	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	2	42	theme	paralog	399:405	arg1	deletion					380:387	The deletion	376:387	The deletion of either paralog	376:405	The deletion of either paralog attenuates virulence in vivo, but the mechanisms of disruption remain unclear.					
30322852	5	43	theme	glucan	964:969	arg1	synthesis					971:979	less insoluble glucan synthesis	949:979	less insoluble glucan synthesis	949:979	Additionally, the deletion of either yidC1 or yidC2 resulted in less insoluble glucan synthesis but produced more soluble glucans, especially at early and mid-exponential-growth phases.					
30322852	7	44	theme	EPS	1325:1327	arg1	matrix					1329:1334	the EPS matrix	1321:1334	the EPS matrix	1321:1334	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	9	45	theme	biofilm	1773:1779	arg1	development					1781:1791	biofilm development	1773:1791	biofilm development	1773:1791	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	10	46	dep	potential	1956:1964	arg1	therapeutic					1966:1976	therapeutic	1966:1976	therapeutic	1966:1976	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	11	47	theme	pathogen	2259:2266	arg1	mutans					2282:2287	the cariogenic pathogen Streptococcus mutans	2244:2287	the cariogenic pathogen Streptococcus mutans	2244:2287	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	4	48	theme	wild	739:742	arg1	type					744:747	the wild type	735:747	the wild type	735:747	Compared to the wild type, the ΔyidC2 mutant lacked staining with fluorescent vancomycin at the division septum, while the ΔyidC1 mutant resembled the wild type.					
30322852	7	49	theme	spatial	1297:1303	arg1	organization					1305:1316	the spatial organization	1293:1316	the spatial organization of the EPS matrix	1293:1334	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	9	50	theme	matrix	1801:1806	arg1	structure/composition					1808:1828	EPS matrix structure/composition	1797:1828	EPS matrix structure/composition	1797:1828	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	12	51	theme	yidC	2492:2495	arg1	deletion					2473:2480	the deletion	2469:2480	the deletion of either yidC	2469:2495	Here, we show that the deletion of either yidC results in changes to the structure and physical properties of the EPS matrix produced by S. mutans, ultimately impairing optimal biofilm development, diminishing its mechanical stability, and facilitating its removal.					
30322852	3	52	theme	EPS	662:664	arg1	matrix					667:672	the exopolysaccharide (EPS) matrix	639:672	the exopolysaccharide (EPS) matrix	639:672	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	0	53	theme	yidC2	31:35	arg1	Biogenesis					58:67	yidC2 Impact Cell Envelope Biogenesis	31:67	yidC2 Impact Cell Envelope Biogenesis	31:67	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	7	54	from	defects	1282:1288	arg1	biomass					1259:1265	biofilm biomass	1251:1265	biofilm biomass	1251:1265	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	7	54	from	defects	1282:1288	arg1	organization					1305:1316	the spatial organization	1293:1316	the spatial organization of the EPS matrix	1293:1334	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	2	55	theme	disruption	459:468	arg1	mechanisms					445:454	the mechanisms	441:454	the mechanisms of disruption	441:468	The deletion of either paralog attenuates virulence in vivo, but the mechanisms of disruption remain unclear.					
30322852	3	56	theme	exopolysaccharide	643:659	arg1	matrix					667:672	the exopolysaccharide (EPS) matrix	639:672	the exopolysaccharide (EPS) matrix	639:672	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	0	57	theme	Cell	44:47	arg1	Biogenesis					58:67	yidC2 Impact Cell Envelope Biogenesis	31:67	yidC2 Impact Cell Envelope Biogenesis	31:67	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	1	58	theme	YidC/Oxa1/Alb3	306:319	arg1	family					321:326	the universally conserved YidC/Oxa1/Alb3 family	280:326	the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases	280:373	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	7	59	theme	pronounced	1271:1280	arg1	defects					1282:1288	pronounced defects	1271:1288	pronounced defects in the spatial organization of the EPS matrix	1271:1334	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	3	60	theme	biophysical	678:688	arg1	properties					690:699	biophysical properties	678:699	biophysical properties of S. mutans biofilm	678:720	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	8	61	theme	defective	1405:1413	arg1	biofilm					1415:1421	The defective biofilm	1401:1421	The defective biofilm	1401:1421	The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear.					
30322852	9	62	theme	physical	1852:1859	arg1	properties					1861:1870	the physical properties	1848:1870	the physical properties of the biofilms	1848:1886	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	3	63	theme	matrix	667:672	arg1	organization					623:634	the structural organization	608:634	the structural organization of the exopolysaccharide (EPS) matrix	608:672	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	0	64	dep	Streptococcus	0:12	arg1	yidC1					21:25	yidC1	21:25	yidC1	21:25	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	0	64	dep	Streptococcus	0:12	arg1	Biogenesis					58:67	yidC2 Impact Cell Envelope Biogenesis	31:67	yidC2 Impact Cell Envelope Biogenesis	31:67	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	0	64	dep	Streptococcus	0:12	arg1	mutans					14:19	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis	0:67	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis	0:67	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	3	65	theme	mutans	707:712	arg1	biofilm					714:720	S. mutans biofilm	704:720	S. mutans biofilm	704:720	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	13	66	theme	orthologs	2774:2782	arg1	conservation					2743:2754	the universal conservation	2729:2754	the universal conservation	2729:2754	Importantly, the universal conservation of bacterial yidC orthologs, combined with our findings, provide a rationale for YidC as a possible drug target for antibiofilm therapies.					
30322852	7	67	theme	significant	1226:1236	arg1	reduction					1238:1246	a significant reduction	1224:1246	a significant reduction in biofilm biomass	1224:1265	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	9	68	theme	biofilms	1879:1886	arg1	properties					1861:1870	the physical properties	1848:1870	the physical properties of the biofilms	1848:1886	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	13	69	theme	antibiofilm	2872:2882	arg1	therapies					2884:2892	antibiofilm therapies	2872:2892	antibiofilm therapies	2872:2892	Importantly, the universal conservation of bacterial yidC orthologs, combined with our findings, provide a rationale for YidC as a possible drug target for antibiofilm therapies.					
30322852	7	70	from	reduction	1238:1246	arg1	biomass					1259:1265	biofilm biomass	1251:1265	biofilm biomass	1251:1265	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	7	70	from	reduction	1238:1246	arg1	organization					1305:1316	the spatial organization	1293:1316	the spatial organization of the EPS matrix	1293:1334	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	12	71	theme	physical	2537:2544	arg1	properties					2546:2555	physical properties	2537:2555	physical properties	2537:2555	Here, we show that the deletion of either yidC results in changes to the structure and physical properties of the EPS matrix produced by S. mutans, ultimately impairing optimal biofilm development, diminishing its mechanical stability, and facilitating its removal.					
30322852	1	72	theme	mutans	170:175	arg1	biogenesis					142:151	Proper envelope biogenesis	126:151	Proper envelope biogenesis of Streptococcus mutans	126:175	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	72	theme	mutans	170:175	arg1	pathogen					227:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	6	73	theme	insoluble	1160:1168	arg1	EPS					1170:1172	insoluble EPS	1160:1172	insoluble EPS	1160:1172	Alteration of glucan synthesis by both mutants yielded biofilms with less dry weight and insoluble EPS.					
30322852	11	74	theme	proper	2306:2311	arg1	biogenesis					2322:2331	proper envelope biogenesis	2306:2331	proper envelope biogenesis	2306:2331	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	10	75	theme	YidC	1920:1923	arg1	proteins					1925:1932	YidC proteins	1920:1932	YidC proteins	1920:1932	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	13	76	theme	bacterial	2759:2767	arg1	orthologs					2774:2782	bacterial yidC orthologs	2759:2782	bacterial yidC orthologs	2759:2782	Importantly, the universal conservation of bacterial yidC orthologs, combined with our findings, provide a rationale for YidC as a possible drug target for antibiofilm therapies.					
30322852	10	77	theme	protein	2190:2196	arg1	insertion					2198:2206	membrane protein insertion	2181:2206	membrane protein insertion	2181:2206	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	10	78	theme	potential	1956:1964	arg1	targets					1978:1984	potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly	1956:2219	potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly	1956:2219	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	3	79	theme	surface	548:554	arg1	properties					556:565	cell surface properties	543:565	cell surface properties	543:565	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	9	80	theme	yidC	1673:1676	arg1	paralog					1678:1684	either yidC paralog	1666:1684	either yidC paralog	1666:1684	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	8	81	theme	bacterial	1440:1448	arg1	clusters					1450:1457	bacterial clusters	1440:1457	bacterial clusters with high cell density	1440:1480	The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear.					
30322852	7	82	theme	biofilm	1379:1385	arg1	architecture					1387:1398	the three-dimensional (3D) biofilm architecture	1352:1398	the three-dimensional (3D) biofilm architecture	1352:1398	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	11	83	theme	full	2337:2340	arg1	virulence					2342:2350	full virulence	2337:2350	full virulence	2337:2350	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	1	84	theme	caries-causing	207:220	arg1	biogenesis					142:151	Proper envelope biogenesis	126:151	Proper envelope biogenesis of Streptococcus mutans	126:175	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	84	theme	caries-causing	207:220	arg1	pathogen					227:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	85	theme	family	321:326	arg1	yidC2					270:274	yidC2	270:274	yidC2	270:274	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	85	theme	family	321:326	arg1	yidC1					260:264	yidC1	260:264	yidC1	260:264	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	85	theme	family	321:326	arg1	paralogs					250:257	two paralogs	246:257	two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases	246:373	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	10	86	theme	cariogenic	1990:1999	arg1	proteins					2033:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	10	86	theme	cariogenic	1990:1999	arg1	insertases					2075:2084	membrane-localized chaperone insertases	2046:2084	membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly	2046:2219	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	3	87	theme	glucan	582:587	arg1	production					589:598	extracellular glucan production	568:598	extracellular glucan production	568:598	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	8	88	theme	cell	1469:1472	arg1	density					1474:1480	high cell density	1464:1480	high cell density	1464:1480	The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear.					
30322852	7	89	theme	three-dimensional	1356:1372	arg1	architecture					1387:1398	the three-dimensional (3D) biofilm architecture	1352:1398	the three-dimensional (3D) biofilm architecture	1352:1398	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	5	90	theme	early	1030:1034	arg1	phases					1063:1068	early and mid-exponential-growth phases	1030:1068	early and mid-exponential-growth phases	1030:1068	Additionally, the deletion of either yidC1 or yidC2 resulted in less insoluble glucan synthesis but produced more soluble glucans, especially at early and mid-exponential-growth phases.					
30322852	1	91	dep	paralogs	250:257	arg1	yidC2					270:274	yidC2	270:274	yidC2	270:274	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	91	dep	paralogs	250:257	arg1	yidC1					260:264	yidC1	260:264	yidC1	260:264	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	91	dep	paralogs	250:257	arg1	paralogs					250:257	two paralogs	246:257	two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases	246:373	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	12	92	theme	mechanical	2664:2673	arg1	stability					2675:2683	its mechanical stability	2660:2683	its mechanical stability	2660:2683	Here, we show that the deletion of either yidC results in changes to the structure and physical properties of the EPS matrix produced by S. mutans, ultimately impairing optimal biofilm development, diminishing its mechanical stability, and facilitating its removal.					
30322852	5	93	theme	mid-exponential-growth	1040:1061	arg1	phases					1063:1068	early and mid-exponential-growth phases	1030:1068	early and mid-exponential-growth phases	1030:1068	Additionally, the deletion of either yidC1 or yidC2 resulted in less insoluble glucan synthesis but produced more soluble glucans, especially at early and mid-exponential-growth phases.					
30322852	1	94	theme	envelope	133:140	arg1	biogenesis					142:151	Proper envelope biogenesis	126:151	Proper envelope biogenesis of Streptococcus mutans	126:175	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	1	94	theme	envelope	133:140	arg1	pathogen					227:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	a biofilm-forming and dental caries-causing oral pathogen	178:234	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	11	95	theme	Streptococcus	2268:2280	arg1	mutans					2282:2287	the cariogenic pathogen Streptococcus mutans	2244:2287	the cariogenic pathogen Streptococcus mutans	2244:2287	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	10	96	theme	control.IMPORTANCE	2009:2026	arg1	proteins					2033:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	10	96	theme	control.IMPORTANCE	2009:2026	arg1	insertases					2075:2084	membrane-localized chaperone insertases	2046:2084	membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly	2046:2219	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	0	97	theme	Biofilm	74:80	arg1	Streptococcus					0:12	Streptococcus	0:12	Streptococcus	0:12	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	0	97	theme	Biofilm	74:80	arg1	Matrix					82:87	the Biofilm Matrix	70:87	the Biofilm Matrix	70:87	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	1	98	theme	membrane	331:338	arg1	chaperones					349:358	membrane integral chaperones	331:358	membrane integral chaperones	331:358	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	12	99	dep	structure	2523:2531	arg1	the					2519:2521	the	2519:2521	the	2519:2521	Here, we show that the deletion of either yidC results in changes to the structure and physical properties of the EPS matrix produced by S. mutans, ultimately impairing optimal biofilm development, diminishing its mechanical stability, and facilitating its removal.					
30322852	9	100	theme	glucan	1730:1735	arg1	production					1737:1746	glucan production	1730:1746	glucan production	1730:1746	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	1	101	theme	chaperones	349:358	arg1	family					321:326	the universally conserved YidC/Oxa1/Alb3 family	280:326	the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases	280:373	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	11	102	theme	biofilm	2415:2421	arg1	formation					2423:2431	optimal biofilm formation	2407:2431	optimal biofilm formation in streptococci	2407:2447	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	4	103	with	staining	775:782	arg1	vancomycin					801:810	fluorescent vancomycin	789:810	fluorescent vancomycin	789:810	Compared to the wild type, the ΔyidC2 mutant lacked staining with fluorescent vancomycin at the division septum, while the ΔyidC1 mutant resembled the wild type.					
30322852	0	104	theme	Biofilm	94:100	arg1	Streptococcus					0:12	Streptococcus	0:12	Streptococcus	0:12	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	0	104	theme	Biofilm	94:100	arg1	Properties					114:123	Biofilm Biophysical Properties	94:123	Biofilm Biophysical Properties	94:123	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	1	105	theme	insertases	364:373	arg1	family					321:326	the universally conserved YidC/Oxa1/Alb3 family	280:326	the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases	280:373	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	13	106	theme	possible	2847:2854	arg1	rationale					2823:2831	a rationale	2821:2831	a rationale for YidC	2821:2840	Importantly, the universal conservation of bacterial yidC orthologs, combined with our findings, provide a rationale for YidC as a possible drug target for antibiofilm therapies.					
30322852	13	106	theme	possible	2847:2854	arg1	target					2861:2866	a possible drug target	2845:2866	a possible drug target for antibiofilm therapies	2845:2892	Importantly, the universal conservation of bacterial yidC orthologs, combined with our findings, provide a rationale for YidC as a possible drug target for antibiofilm therapies.					
30322852	5	107	theme	insoluble	954:962	arg1	synthesis					971:979	less insoluble glucan synthesis	949:979	less insoluble glucan synthesis	949:979	Additionally, the deletion of either yidC1 or yidC2 resulted in less insoluble glucan synthesis but produced more soluble glucans, especially at early and mid-exponential-growth phases.					
30322852	10	108	theme	membrane-localized	2046:2063	arg1	proteins					2033:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	cariogenic biofilm control.IMPORTANCE YidC proteins	1990:2040	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	10	108	theme	membrane-localized	2046:2063	arg1	insertases					2075:2084	membrane-localized chaperone insertases	2046:2084	membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly	2046:2219	YidC proteins, therefore, represent potential therapeutic targets for cariogenic biofilm control.IMPORTANCE YidC proteins are membrane-localized chaperone insertases that are universally conserved in all bacteria and are traditionally studied in the context of membrane protein insertion and assembly.					
30322852	8	109	theme	wild	1525:1528	arg1	type					1530:1533	the wild type	1521:1533	the wild type	1521:1533	The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear.					
30322852	8	109	theme	wild	1525:1528	arg1	stiffer					1546:1552	stiffer	1546:1552	stiffer	1546:1552	The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear.					
30322852	5	110	theme	yidC1	922:926	arg1	deletion					903:910	the deletion	899:910	the deletion of either yidC1 or yidC2	899:935	Additionally, the deletion of either yidC1 or yidC2 resulted in less insoluble glucan synthesis but produced more soluble glucans, especially at early and mid-exponential-growth phases.					
30322852	12	111	theme	optimal	2619:2625	arg1	development					2635:2645	optimal biofilm development	2619:2645	optimal biofilm development	2619:2645	Here, we show that the deletion of either yidC results in changes to the structure and physical properties of the EPS matrix produced by S. mutans, ultimately impairing optimal biofilm development, diminishing its mechanical stability, and facilitating its removal.					
30322852	5	112	theme	yidC2	931:935	arg1	deletion					903:910	the deletion	899:910	the deletion of either yidC1 or yidC2	899:935	Additionally, the deletion of either yidC1 or yidC2 resulted in less insoluble glucan synthesis but produced more soluble glucans, especially at early and mid-exponential-growth phases.					
30322852	9	113	theme	EPS	1797:1799	arg1	structure/composition					1808:1828	EPS matrix structure/composition	1797:1828	EPS matrix structure/composition	1797:1828	Together, our results indicate that the elimination of either yidC paralog results in changes to the cell envelope and glucan production that ultimately disrupts biofilm development and EPS matrix structure/composition, thereby altering the physical properties of the biofilms and facilitating their removal.					
30322852	0	114	theme	Impact	37:42	arg1	Biogenesis					58:67	yidC2 Impact Cell Envelope Biogenesis	31:67	yidC2 Impact Cell Envelope Biogenesis	31:67	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	5	115	theme	soluble	999:1005	arg1	glucans					1007:1013	more soluble glucans	994:1013	more soluble glucans	994:1013	Additionally, the deletion of either yidC1 or yidC2 resulted in less insoluble glucan synthesis but produced more soluble glucans, especially at early and mid-exponential-growth phases.					
30322852	0	116	theme	Envelope	49:56	arg1	Biogenesis					58:67	yidC2 Impact Cell Envelope Biogenesis	31:67	yidC2 Impact Cell Envelope Biogenesis	31:67	Streptococcus mutans yidC1 and yidC2 Impact Cell Envelope Biogenesis, the Biofilm Matrix, and Biofilm Biophysical Properties.					
30322852	1	117	theme	conserved	296:304	arg1	family					321:326	the universally conserved YidC/Oxa1/Alb3 family	280:326	the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases	280:373	Proper envelope biogenesis of Streptococcus mutans, a biofilm-forming and dental caries-causing oral pathogen, requires two paralogs (yidC1 and yidC2) of the universally conserved YidC/Oxa1/Alb3 family of membrane integral chaperones and insertases.					
30322852	11	118	theme	YidC	2227:2230	arg1	paralogs					2232:2239	Both YidC paralogs	2222:2239	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans	2222:2287	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	4	119	theme	division	819:826	arg1	septum					828:833	the division septum	815:833	the division septum	815:833	Compared to the wild type, the ΔyidC2 mutant lacked staining with fluorescent vancomycin at the division septum, while the ΔyidC1 mutant resembled the wild type.					
30322852	8	120	with	clusters	1450:1457	arg1	density					1474:1480	high cell density	1464:1480	high cell density	1464:1480	The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear.					
30322852	8	121	dep	smaller	1432:1438	arg1	clusters					1450:1457	bacterial clusters	1440:1457	bacterial clusters with high cell density	1440:1480	The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear.					
30322852	8	121	dep	smaller	1432:1438	arg1	EPS					1503:1505	EPS	1503:1505	EPS	1503:1505	The defective biofilm harbored smaller bacterial clusters with high cell density and less surrounding EPS than those of the wild type, which was stiffer in compression yet more susceptible to removal by shear.					
30322852	7	122	theme	biofilm	1251:1257	arg1	biomass					1259:1265	biofilm biomass	1251:1265	biofilm biomass	1251:1265	In particular, the deletion of yidC2 resulted in a significant reduction in biofilm biomass and pronounced defects in the spatial organization of the EPS matrix, thus modifying the three-dimensional (3D) biofilm architecture.					
30322852	3	123	theme	S.	704:705	arg1	biofilm					714:720	S. mutans biofilm	704:720	S. mutans biofilm	704:720	Here, we determined whether the deletion of yidC affects cell surface properties, extracellular glucan production, and/or the structural organization of the exopolysaccharide (EPS) matrix and biophysical properties of S. mutans biofilm.					
30322852	4	124	theme	fluorescent	789:799	arg1	vancomycin					801:810	fluorescent vancomycin	789:810	fluorescent vancomycin	789:810	Compared to the wild type, the ΔyidC2 mutant lacked staining with fluorescent vancomycin at the division septum, while the ΔyidC1 mutant resembled the wild type.					
30322852	11	125	from	formation	2423:2431	arg1	streptococci					2436:2447	streptococci	2436:2447	streptococci	2436:2447	Both YidC paralogs of the cariogenic pathogen Streptococcus mutans are required for proper envelope biogenesis and full virulence, indicating that these proteins may also contribute to optimal biofilm formation in streptococci.					
30322852	13	126	theme	yidC	2769:2772	arg1	orthologs					2774:2782	bacterial yidC orthologs	2759:2782	bacterial yidC orthologs	2759:2782	Importantly, the universal conservation of bacterial yidC orthologs, combined with our findings, provide a rationale for YidC as a possible drug target for antibiofilm therapies.					
29524817	6	0	theme	acidic	1629:1634	arg1	nature					1636:1641	more acidic nature	1624:1641	more acidic nature	1624:1641	The molecules of more acidic nature tended to remain in the permeate of effluent DOM, while the river DOM was shifted into more nitrogen-enriched composition after filtration.					
29524817	5	1	theme	DOM	1539:1541	arg1	composition					1524:1534	the overall molecular composition	1502:1534	the overall molecular composition of DOM upon UF filtration	1502:1560	The changes in the overall molecular composition of DOM upon UF filtration were highly dependent on the sources of DOM.					
29524817	0	2	theme	DOM	155:157	arg1	tools					176:180	multiple advanced DOM characterization tools	137:180	multiple advanced DOM characterization tools	137:180	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	7	3	theme	DOM	1923:1925	arg1	composition					1908:1918	the molecular composition	1894:1918	the molecular composition of DOM	1894:1925	Regardless of the DOM sources, the HPO membrane with a smaller pore size led to the most pronounced changes in the molecular composition of DOM.					
29524817	2	4	theme	UF	769:770	arg1	membranes					772:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	2	4	theme	UF	769:770	arg1	membranes					626:634	Two polyethersulfone membranes	605:634	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane	605:699	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	1	5	theme	organic	268:274	arg1	DOM					284:286	DOM	284:286	DOM	284:286	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	5	theme	organic	268:274	arg1	matter					276:281	dissolved organic matter	258:281	dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent)	258:347	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	5	6	theme	overall	1506:1512	arg1	composition					1524:1534	the overall molecular composition	1502:1534	the overall molecular composition of DOM upon UF filtration	1502:1560	The changes in the overall molecular composition of DOM upon UF filtration were highly dependent on the sources of DOM.					
29524817	4	7	theme	formula	1347:1353	arg1	group					1355:1359	the CHOS formula group	1338:1359	the CHOS formula group	1338:1359	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	1	8	theme	emission	433:440	arg1	chromatography					506:519	size exclusion chromatography	491:519	size exclusion chromatography (SEC)	491:525	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	8	theme	emission	433:440	arg1	matrix					442:447	excitation emission matrix	422:447	excitation emission matrix	422:447	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	3	9	dep	components	925:934	arg1	microbial-humic-like					962:981	microbial-humic-like	962:981	microbial-humic-like	962:981	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	3	9	dep	components	925:934	arg1	humic-like					1000:1009	humic-like	1000:1009	humic-like	1000:1009	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	3	9	dep	components	925:934	arg1	tryptophan-like					941:955	tryptophan-like	941:955	tryptophan-like	941:955	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	3	10	theme	DOM	1257:1259	arg1	molecules					1261:1269	small sized DOM molecules	1245:1269	small sized DOM molecules	1245:1269	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	3	11	theme	UF	1209:1210	arg1	fouling					1221:1227	UF membrane fouling	1209:1227	UF membrane fouling	1209:1227	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	1	12	dep	different	233:241	arg1	constitutes					243:253	constitutes	243:253	constitutes	243:253	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	3	13	theme	small	1245:1249	arg1	molecules					1261:1269	small sized DOM molecules	1245:1269	small sized DOM molecules	1245:1269	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	5	14	theme	UF	1548:1549	arg1	filtration					1551:1560	UF filtration	1548:1560	UF filtration	1548:1560	The changes in the overall molecular composition of DOM upon UF filtration were highly dependent on the sources of DOM.					
29524817	1	15	from	different	233:241	arg1	DOM					284:286	DOM	284:286	DOM	284:286	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	15	from	different	233:241	arg1	matter					276:281	dissolved organic matter	258:281	dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent)	258:347	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	3	16	theme	size	806:809	arg1	exclusion					811:819	size exclusion	806:819	size exclusion effect	806:826	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	2	17	theme	pore	651:654	arg1	sizes					656:660	different pore sizes	641:660	different pore sizes	641:660	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	1	18	from	contributions	216:228	arg1	DOM					284:286	DOM	284:286	DOM	284:286	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	18	from	contributions	216:228	arg1	matter					276:281	dissolved organic matter	258:281	dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent)	258:347	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	19	theme	cyclotron	554:562	arg1	FT-ICR-MS					593:601	FT-ICR-MS	593:601	FT-ICR-MS	593:601	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	19	theme	cyclotron	554:562	arg1	spectrometry					579:590	ion cyclotron resonance mass spectrometry	550:590	ion cyclotron resonance mass spectrometry (FT-ICR-MS)	550:602	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	0	20	theme	composition	47:57	arg1	roles					25:29	the roles	21:29	the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes	21:120	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	2	21	theme	regenerated	670:680	arg1	membrane					692:699	one regenerated cellulose membrane	666:699	one regenerated cellulose membrane	666:699	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	1	22	theme	mass	574:577	arg1	FT-ICR-MS					593:601	FT-ICR-MS	593:601	FT-ICR-MS	593:601	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	22	theme	mass	574:577	arg1	spectrometry					579:590	ion cyclotron resonance mass spectrometry	550:590	ion cyclotron resonance mass spectrometry (FT-ICR-MS)	550:602	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	4	23	theme	greater	1370:1376	arg1	tendency					1386:1393	a greater removal tendency	1368:1393	a greater removal tendency toward the HPO membrane	1368:1417	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	0	24	theme	dissolved	62:70	arg1	DOM					88:90	DOM	88:90	DOM	88:90	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	0	24	theme	dissolved	62:70	arg1	matter					80:85	dissolved organic matter	62:85	dissolved organic matter (DOM)	62:91	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	3	25	theme	fractions	1031:1039	arg1	behaviors					889:897	the behaviors	885:897	the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration	885:1062	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	0	26	theme	matter	80:85	arg1	composition					47:57	the chemical composition	34:57	the chemical composition of dissolved organic matter (DOM)	34:91	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	3	27	theme	different	1016:1024	arg1	fractions					1031:1039	different size fractions	1016:1039	different size fractions	1016:1039	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	6	28	dep	the	1663:1665	arg1	permeate					1667:1674	permeate	1667:1674	permeate	1667:1674	The molecules of more acidic nature tended to remain in the permeate of effluent DOM, while the river DOM was shifted into more nitrogen-enriched composition after filtration.					
29524817	1	29	theme	exclusion	496:504	arg1	chromatography					506:519	size exclusion chromatography	491:519	size exclusion chromatography (SEC)	491:525	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	29	theme	exclusion	496:504	arg1	SEC					522:524	SEC	522:524	SEC	522:524	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	29	theme	exclusion	496:504	arg1	matrix					442:447	excitation emission matrix	422:447	excitation emission matrix	422:447	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	0	30	from	roles	25:29	arg1	membranes					112:120	ultrafiltration membranes	96:120	ultrafiltration membranes	96:120	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	0	31	theme	Further	0:6	arg1	insight					8:14	Further insight	0:14	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes	0:120	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	6	32	theme	river	1703:1707	arg1	DOM					1709:1711	the river DOM	1699:1711	the river DOM	1699:1711	The molecules of more acidic nature tended to remain in the permeate of effluent DOM, while the river DOM was shifted into more nitrogen-enriched composition after filtration.					
29524817	2	33	theme	polyethersulfone	609:624	arg1	membranes					626:634	Two polyethersulfone membranes	605:634	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane	605:699	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	2	33	theme	polyethersulfone	609:624	arg1	membranes					772:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	3	34	theme	rejection	864:872	arg1	mechanism					874:882	the most prevailing rejection mechanism	844:882	the most prevailing rejection mechanism	844:882	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	3	34	theme	rejection	864:872	arg1	effect					821:826	size exclusion effect	806:826	size exclusion effect	806:826	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	4	35	theme	CHONS	1430:1434	arg1	prone					1446:1450	prone	1446:1450	prone	1446:1450	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	4	35	theme	CHONS	1430:1434	arg1	group					1436:1440	the CHONS group	1426:1440	the CHONS group	1426:1440	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	5	36	theme	DOM	1602:1604	arg1	sources					1591:1597	the sources	1587:1597	the sources of DOM	1587:1604	The changes in the overall molecular composition of DOM upon UF filtration were highly dependent on the sources of DOM.					
29524817	5	37	from	changes	1491:1497	arg1	composition					1524:1534	the overall molecular composition	1502:1534	the overall molecular composition of DOM upon UF filtration	1502:1560	The changes in the overall molecular composition of DOM upon UF filtration were highly dependent on the sources of DOM.					
29524817	3	38	theme	individual	902:911	arg1	components					925:934	individual fluorescent components	902:934	individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like)	902:1010	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	1	39	theme	parallel	451:458	arg1	EEM-PARAFAC					477:487	EEM-PARAFAC	477:487	EEM-PARAFAC	477:487	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	39	theme	parallel	451:458	arg1	analysis					467:474	parallel factor analysis	451:474	parallel factor analysis (EEM-PARAFAC)	451:488	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	40	theme	different	298:306	arg1	sources					308:314	two different sources	294:314	two different sources	294:314	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	3	41	theme	hydrophobic	1107:1117	arg1	interactions					1119:1130	hydrophobic interactions	1107:1130	hydrophobic interactions	1107:1130	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	1	42	theme	relative	207:214	arg1	contributions					216:228	the relative contributions	203:228	the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC),	203:526	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	5	43	theme	molecular	1514:1522	arg1	composition					1524:1534	the overall molecular composition	1502:1534	the overall molecular composition of DOM upon UF filtration	1502:1560	The changes in the overall molecular composition of DOM upon UF filtration were highly dependent on the sources of DOM.					
29524817	3	44	theme	components	925:934	arg1	behaviors					889:897	the behaviors	885:897	the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration	885:1062	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	6	45	theme	nitrogen-enriched	1735:1751	arg1	composition					1753:1763	more nitrogen-enriched composition	1730:1763	more nitrogen-enriched composition	1730:1763	The molecules of more acidic nature tended to remain in the permeate of effluent DOM, while the river DOM was shifted into more nitrogen-enriched composition after filtration.					
29524817	4	46	theme	molecular	1285:1293	arg1	composition					1301:1311	the molecular level composition	1281:1311	the molecular level composition determined by FT-ICR-MS	1281:1335	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	0	47	theme	characterization	159:174	arg1	tools					176:180	multiple advanced DOM characterization tools	137:180	multiple advanced DOM characterization tools	137:180	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	1	48	theme	UF	404:405	arg1	membranes					408:416	ultrafiltration (UF) membranes	387:416	ultrafiltration (UF) membranes	387:416	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	49	theme	dissolved	258:266	arg1	DOM					284:286	DOM	284:286	DOM	284:286	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	49	theme	dissolved	258:266	arg1	matter					276:281	dissolved organic matter	258:281	dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent)	258:347	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	7	50	from	changes	1883:1889	arg1	composition					1908:1918	the molecular composition	1894:1918	the molecular composition of DOM	1894:1925	Regardless of the DOM sources, the HPO membrane with a smaller pore size led to the most pronounced changes in the molecular composition of DOM.					
29524817	2	51	theme	hydrophobic	729:739	arg1	membranes					772:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	2	51	theme	hydrophobic	729:739	arg1	membranes					626:634	Two polyethersulfone membranes	605:634	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane	605:699	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	1	52	theme	membranes	408:416	arg1	types					378:382	three types	372:382	three types of ultrafiltration (UF) membranes	372:416	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	7	53	theme	molecular	1898:1906	arg1	composition					1908:1918	the molecular composition	1894:1918	the molecular composition of DOM	1894:1925	Regardless of the DOM sources, the HPO membrane with a smaller pore size led to the most pronounced changes in the molecular composition of DOM.					
29524817	6	54	theme	effluent	1679:1686	arg1	DOM					1688:1690	effluent DOM	1679:1690	effluent DOM	1679:1690	The molecules of more acidic nature tended to remain in the permeate of effluent DOM, while the river DOM was shifted into more nitrogen-enriched composition after filtration.					
29524817	1	55	from	matter	276:281	arg1	different					233:241	different	233:241	different	233:241	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	55	from	matter	276:281	arg1	contributions					216:228	the relative contributions	203:228	the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC),	203:526	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	56	theme	excitation	422:431	arg1	chromatography					506:519	size exclusion chromatography	491:519	size exclusion chromatography (SEC)	491:525	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	56	theme	excitation	422:431	arg1	matrix					442:447	excitation emission matrix	422:447	excitation emission matrix	422:447	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	3	57	theme	important	1190:1198	arg1	roles					1200:1204	important roles	1190:1204	important roles	1190:1204	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	1	58	with	matter	276:281	arg1	sources					308:314	two different sources	294:314	two different sources	294:314	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	4	59	theme	CHOS	1342:1345	arg1	group					1355:1359	the CHOS formula group	1338:1359	the CHOS formula group	1338:1359	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	0	60	theme	ultrafiltration	96:110	arg1	membranes					112:120	ultrafiltration membranes	96:120	ultrafiltration membranes	96:120	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	1	61	theme	membrane	352:359	arg1	fouling					361:367	membrane fouling	352:367	membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC),	352:526	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	3	62	theme	membrane	1212:1219	arg1	fouling					1221:1227	UF membrane fouling	1209:1227	UF membrane fouling	1209:1227	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	3	63	theme	sized	1251:1255	arg1	molecules					1261:1269	small sized DOM molecules	1245:1269	small sized DOM molecules	1245:1269	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	2	64	with	membranes	626:634	arg1	membrane					692:699	one regenerated cellulose membrane	666:699	one regenerated cellulose membrane	666:699	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	2	64	with	membranes	626:634	arg1	sizes					656:660	different pore sizes	641:660	different pore sizes	641:660	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	3	65	dep	interactions	1119:1130	arg1	e.g.					1101:1104	e.g.	1101:1104	e.g.	1101:1104	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	7	66	with	membrane	1822:1829	arg1	size					1851:1854	a smaller pore size	1836:1854	a smaller pore size	1836:1854	Regardless of the DOM sources, the HPO membrane with a smaller pore size led to the most pronounced changes in the molecular composition of DOM.					
29524817	0	67	theme	advanced	146:153	arg1	tools					176:180	multiple advanced DOM characterization tools	137:180	multiple advanced DOM characterization tools	137:180	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	2	68	used	used	706:709	arg2	membranes					626:634	Two polyethersulfone membranes	605:634	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane	605:699	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	2	68	used	used	706:709	arg2	membranes					772:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	3	69	theme	exclusion	811:819	arg1	mechanism					874:882	the most prevailing rejection mechanism	844:882	the most prevailing rejection mechanism	844:882	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	3	69	theme	exclusion	811:819	arg1	effect					821:826	size exclusion effect	806:826	size exclusion effect	806:826	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	1	70	theme	ion	550:552	arg1	FT-ICR-MS					593:601	FT-ICR-MS	593:601	FT-ICR-MS	593:601	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	70	theme	ion	550:552	arg1	spectrometry					579:590	ion cyclotron resonance mass spectrometry	550:590	ion cyclotron resonance mass spectrometry (FT-ICR-MS)	550:602	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	71	theme	ultrafiltration	387:401	arg1	membranes					408:416	ultrafiltration (UF) membranes	387:416	ultrafiltration (UF) membranes	387:416	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	2	72	theme	cellulose	682:690	arg1	membrane					692:699	one regenerated cellulose membrane	666:699	one regenerated cellulose membrane	666:699	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	1	73	theme	resonance	564:572	arg1	FT-ICR-MS					593:601	FT-ICR-MS	593:601	FT-ICR-MS	593:601	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	73	theme	resonance	564:572	arg1	spectrometry					579:590	ion cyclotron resonance mass spectrometry	550:590	ion cyclotron resonance mass spectrometry (FT-ICR-MS)	550:602	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	4	74	theme	HPO	1406:1408	arg1	membrane					1410:1417	the HPO membrane	1402:1417	the HPO membrane	1402:1417	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	0	75	theme	organic	72:78	arg1	DOM					88:90	DOM	88:90	DOM	88:90	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	0	75	theme	organic	72:78	arg1	matter					80:85	dissolved organic matter	62:85	dissolved organic matter (DOM)	62:91	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	3	76	theme	size	1026:1029	arg1	fractions					1031:1039	different size fractions	1016:1039	different size fractions	1016:1039	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	7	77	theme	pore	1846:1849	arg1	size					1851:1854	a smaller pore size	1836:1854	a smaller pore size	1836:1854	Regardless of the DOM sources, the HPO membrane with a smaller pore size led to the most pronounced changes in the molecular composition of DOM.					
29524817	1	78	from	fouling	361:367	arg1	types					378:382	three types	372:382	three types of ultrafiltration (UF) membranes	372:416	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	79	theme	urban	323:327	arg1	river					329:333	urban river	323:333	urban river	323:333	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	7	80	theme	smaller	1838:1844	arg1	size					1851:1854	a smaller pore size	1836:1854	a smaller pore size	1836:1854	Regardless of the DOM sources, the HPO membrane with a smaller pore size led to the most pronounced changes in the molecular composition of DOM.					
29524817	0	81	theme	multiple	137:144	arg1	tools					176:180	multiple advanced DOM characterization tools	137:180	multiple advanced DOM characterization tools	137:180	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	1	82	theme	size	491:494	arg1	chromatography					506:519	size exclusion chromatography	491:519	size exclusion chromatography (SEC)	491:525	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	82	theme	size	491:494	arg1	SEC					522:524	SEC	522:524	SEC	522:524	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	82	theme	size	491:494	arg1	matrix					442:447	excitation emission matrix	422:447	excitation emission matrix	422:447	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	4	83	theme	removal	1378:1384	arg1	tendency					1386:1393	a greater removal tendency	1368:1393	a greater removal tendency toward the HPO membrane	1368:1417	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	3	84	theme	UF	1050:1051	arg1	filtration					1053:1062	the UF filtration	1046:1062	the UF filtration	1046:1062	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	3	85	theme	prevailing	853:862	arg1	mechanism					874:882	the most prevailing rejection mechanism	844:882	the most prevailing rejection mechanism	844:882	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	3	85	theme	prevailing	853:862	arg1	effect					821:826	size exclusion effect	806:826	size exclusion effect	806:826	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	6	86	theme	nature	1636:1641	arg1	molecules					1611:1619	The molecules	1607:1619	The molecules of more acidic nature	1607:1641	The molecules of more acidic nature tended to remain in the permeate of effluent DOM, while the river DOM was shifted into more nitrogen-enriched composition after filtration.					
29524817	2	87	theme	different	641:649	arg1	sizes					656:660	different pore sizes	641:660	different pore sizes	641:660	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	7	88	theme	HPO	1818:1820	arg1	membrane					1822:1829	the HPO membrane	1814:1829	the HPO membrane with a smaller pore size	1814:1854	Regardless of the DOM sources, the HPO membrane with a smaller pore size led to the most pronounced changes in the molecular composition of DOM.					
29524817	3	89	theme	chemical	1078:1085	arg1	interactions					1087:1098	chemical interactions	1078:1098	chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane	1078:1177	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	7	90	theme	DOM	1801:1803	arg1	sources					1805:1811	the DOM sources	1797:1811	the DOM sources	1797:1811	Regardless of the DOM sources, the HPO membrane with a smaller pore size led to the most pronounced changes in the molecular composition of DOM.					
29524817	7	91	theme	pronounced	1872:1881	arg1	changes					1883:1889	the most pronounced changes	1863:1889	the most pronounced changes in the molecular composition of DOM	1863:1925	Regardless of the DOM sources, the HPO membrane with a smaller pore size led to the most pronounced changes in the molecular composition of DOM.					
29524817	0	92	theme	chemical	38:45	arg1	composition					47:57	the chemical composition	34:57	the chemical composition of dissolved organic matter (DOM)	34:91	Further insight into the roles of the chemical composition of dissolved organic matter (DOM) on ultrafiltration membranes as revealed by multiple advanced DOM characterization tools.					
29524817	3	93	theme	fluorescent	913:923	arg1	components					925:934	individual fluorescent components	902:934	individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like)	902:1010	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	1	94	dep	river	329:333	arg1	i.e.					317:320	i.e.	317:320	i.e.	317:320	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	95	theme	factor	460:465	arg1	EEM-PARAFAC					477:487	EEM-PARAFAC	477:487	EEM-PARAFAC	477:487	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	1	95	theme	factor	460:465	arg1	analysis					467:474	parallel factor analysis	451:474	parallel factor analysis (EEM-PARAFAC)	451:488	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
29524817	4	96	theme	HPI	1473:1475	arg1	membrane					1477:1484	the HPI membrane	1469:1484	the HPI membrane	1469:1484	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	2	97	dep	hydrophobic	729:739	arg1	HPI					764:766	HPI	764:766	HPI	764:766	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	2	97	dep	hydrophobic	729:739	arg1	HPO					742:744	HPO	742:744	HPO	742:744	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	3	98	theme	hydrogen	1136:1143	arg1	bonding					1145:1151	hydrogen bonding	1136:1151	hydrogen bonding	1136:1151	Although size exclusion effect was found to be the most prevailing rejection mechanism, the behaviors of individual fluorescent components (one tryptophan-like, one microbial-humic-like, and terrestrial humic-like) and different size fractions upon the UF filtration revealed that chemical interactions (e.g., hydrophobic interactions and hydrogen bonding) between DOM and membrane might play important roles in UF membrane fouling, especially for small sized DOM molecules.					
29524817	2	99	theme	hydrophilic	751:761	arg1	membranes					772:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes	714:780	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	2	99	theme	hydrophilic	751:761	arg1	membranes					626:634	Two polyethersulfone membranes	605:634	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane	605:699	Two polyethersulfone membranes with different pore sizes and one regenerated cellulose membrane were used as representative hydrophobic (HPO) and hydrophilic (HPI) UF membranes, respectively.					
29524817	4	100	theme	level	1295:1299	arg1	composition					1301:1311	the molecular level composition	1281:1311	the molecular level composition determined by FT-ICR-MS	1281:1335	Based on the molecular level composition determined by FT-ICR-MS, the CHOS formula group showed a greater removal tendency toward the HPO membrane, while the CHONS group was prone to be removed by the HPI membrane.					
29524817	1	101	theme	different	233:241	arg1	contributions					216:228	the relative contributions	203:228	the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC),	203:526	This study assessed the relative contributions of different constitutes in dissolved organic matter (DOM) with two different sources (i.e., urban river and effluent) to membrane fouling on three types of ultrafiltration (UF) membranes via excitation emission matrix - parallel factor analysis (EEM-PARAFAC), size exclusion chromatography (SEC), and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).					
31362158	2	0	theme	polished	332:339	arg1	rice					341:344	polished rice	332:344	polished rice containing high total dietary fiber (TDF)	332:386	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	1	1	theme	attention	241:249	arg1	amounts					230:236	increasing amounts	219:236	increasing amounts of attention	219:249	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	1	1	theme	attention	241:249	arg1	attention					241:249	attention	241:249	attention	241:249	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	5	2	with	rice	934:937	arg1	content					955:961	higher TDF content	944:961	higher TDF content	944:961	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	1	3	theme	healthier	147:155	arg1	rice					157:160	healthier rice	147:160	healthier rice	147:160	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	5	4	theme	high	1070:1073	arg1	breeding					1084:1091	high TDF rice breeding	1070:1091	high TDF rice breeding	1070:1091	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	2	5	theme	AOAC	275:278	arg1	method					265:270	the method	261:270	the method of AOAC 2002.02	261:286	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	4	6	contain	had	823:825	arg2	content					831:837	TDF content	827:837	TDF content lower than 5%	827:851	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	4	6	contain	had	823:825	arg1	those					777:781	those	777:781	those	777:781	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	5	7	theme	digestion	871:879	arg1	resistance					881:890	digestion resistance	871:890	digestion resistance	871:890	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	1	8	with	development	132:142	arg1	composition					193:203	an improved dietary fiber composition	167:203	an improved dietary fiber composition	167:203	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	4	9	theme	digestion	714:722	arg1	resistance					724:733	digestion resistance	714:733	digestion resistance	714:733	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	3	10	theme	Mutant	389:394	arg1	cw					396:397	Mutant cw	389:397	Mutant cw with a high TDF content	389:421	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	1	11	theme	rice	157:160	arg1	development					132:142	the development	128:142	the development of healthier rice with an improved dietary fiber composition	128:203	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	1	12	theme	Dietary	77:83	arg1	fiber					85:89	Dietary fiber	77:89	Dietary fiber	77:89	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	2	13	theme	dietary	368:374	arg1	TDF					383:385	TDF	383:385	TDF	383:385	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	2	13	theme	dietary	368:374	arg1	fiber					376:380	high total dietary fiber	357:380	high total dietary fiber (TDF)	357:386	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	2	14	contain	containing	346:355	arg2	fiber					376:380	high total dietary fiber	357:380	high total dietary fiber (TDF)	357:386	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	2	14	contain	containing	346:355	arg2	TDF					383:385	TDF	383:385	TDF	383:385	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	2	14	contain	containing	346:355	arg1	rice					341:344	polished rice	332:344	polished rice containing high total dietary fiber (TDF)	332:386	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	4	15	theme	digestion-resistant	793:811	arg1	phenotype					813:821	a digestion-resistant phenotype	791:821	a digestion-resistant phenotype	791:821	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	4	16	theme	F2	693:694	arg1	population					696:705	the F2 population	689:705	the F2 population	689:705	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	4	17	theme	lower	839:843	arg1	content					831:837	TDF content	827:837	TDF content lower than 5%	827:851	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	2	18	theme	total	362:366	arg1	TDF					383:385	TDF	383:385	TDF	383:385	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	2	18	theme	total	362:366	arg1	fiber					376:380	high total dietary fiber	357:380	high total dietary fiber (TDF)	357:386	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	0	19	theme	High-throughput	0:14	arg1	method					16:21	High-throughput method	0:21	High-throughput method for preliminary screening of high dietary fiber rice.	0:75	High-throughput method for preliminary screening of high dietary fiber rice.					
31362158	3	20	theme	digestion-resistant	506:524	arg1	phenotype					526:534	the digestion-resistant phenotype	502:534	the digestion-resistant phenotype	502:534	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	3	20	theme	digestion-resistant	506:524	arg1	grain					580:584	an almost intact grain	563:584	an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase	563:654	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	1	21	theme	improved	170:177	arg1	composition					193:203	an improved dietary fiber composition	167:203	an improved dietary fiber composition	167:203	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	0	22	theme	preliminary	27:37	arg1	screening					39:47	preliminary screening	27:47	preliminary screening of high dietary fiber rice	27:74	High-throughput method for preliminary screening of high dietary fiber rice.					
31362158	1	23	theme	several	95:101	arg1	benefits					103:110	several benefits	95:110	several benefits for humans	95:121	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	1	24	theme	dietary	179:185	arg1	composition					193:203	an improved dietary fiber composition	167:203	an improved dietary fiber composition	167:203	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	5	25	theme	resistance	881:890	arg1	index					912:916	a valuable index	901:916	a valuable index for identifying rice with higher TDF content	901:961	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	5	25	theme	resistance	881:890	arg1	phenotype					858:866	The phenotype	854:866	The phenotype of digestion resistance	854:890	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	5	26	theme	phenotype	995:1003	arg1	identification					972:985	the identification	968:985	the identification of this phenotype	968:1003	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	1	27	theme	fiber	187:191	arg1	composition					193:203	an improved dietary fiber composition	167:203	an improved dietary fiber composition	167:203	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	5	28	theme	TDF	951:953	arg1	content					955:961	higher TDF content	944:961	higher TDF content	944:961	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	2	29	theme	high	357:360	arg1	TDF					383:385	TDF	383:385	TDF	383:385	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	2	29	theme	high	357:360	arg1	fiber					376:380	high total dietary fiber	357:380	high total dietary fiber (TDF)	357:386	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	3	30	theme	high	406:409	arg1	content					415:421	a high TDF content	404:421	a high TDF content	404:421	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	5	31	theme	TDF	1075:1077	arg1	breeding					1084:1091	high TDF rice breeding	1070:1091	high TDF rice breeding	1070:1091	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	3	32	theme	intact	573:578	arg1	phenotype					526:534	the digestion-resistant phenotype	502:534	the digestion-resistant phenotype	502:534	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	3	32	theme	intact	573:578	arg1	grain					580:584	an almost intact grain	563:584	an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase	563:654	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	3	33	theme	TDF	411:413	arg1	content					415:421	a high TDF content	404:421	a high TDF content	404:421	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	0	34	theme	dietary	57:63	arg1	rice					71:74	high dietary fiber rice	52:74	high dietary fiber rice	52:74	High-throughput method for preliminary screening of high dietary fiber rice.					
31362158	5	35	theme	rice	1079:1082	arg1	breeding					1084:1091	high TDF rice breeding	1070:1091	high TDF rice breeding	1070:1091	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	5	36	theme	simplified	1016:1025	arg1	method					1059:1064	a simplified, economical and high throughput method	1014:1064	a simplified, economical and high throughput method for high TDF rice breeding	1014:1091	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	5	37	theme	valuable	903:910	arg1	phenotype					858:866	The phenotype	854:866	The phenotype of digestion resistance	854:890	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	5	37	theme	valuable	903:910	arg1	index					912:916	a valuable index	901:916	a valuable index for identifying rice with higher TDF content	901:961	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	5	38	dep	simplified	1016:1025	arg1	high					1043:1046	high	1043:1046	high	1043:1046	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	5	38	dep	simplified	1016:1025	arg1	economical					1028:1037	economical	1028:1037	economical	1028:1037	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	4	39	dep	showed	707:712	arg1	had					739:741	had	739:741	had	739:741	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	0	40	theme	high	52:55	arg1	rice					71:74	high dietary fiber rice	52:74	high dietary fiber rice	52:74	High-throughput method for preliminary screening of high dietary fiber rice.					
31362158	1	41	contain	has	91:93	arg2	benefits					103:110	several benefits	95:110	several benefits for humans	95:121	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	1	41	contain	has	91:93	arg1	fiber					85:89	Dietary fiber	77:89	Dietary fiber	77:89	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	3	42	with	cw	396:397	arg1	content					415:421	a high TDF content	404:421	a high TDF content	404:421	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	3	43	theme	pancreatic	614:623	arg1	α-amylase					625:633	pancreatic α-amylase	614:633	pancreatic α-amylase	614:633	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	2	44	theme	simplified	304:313	arg1	method					315:320	a simplified method	302:320	a simplified method to screen polished rice containing high total dietary fiber (TDF)	302:386	Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF).					
31362158	4	45	theme	TDF	743:745	arg1	content					747:753	TDF content	743:753	TDF content	743:753	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	3	46	dep	japonica	489:496	arg1	Nipponbare					477:486	Nipponbare	477:486	Nipponbare	477:486	Mutant cw with a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase.					
31362158	4	47	theme	TDF	827:829	arg1	content					831:837	TDF content	827:837	TDF content lower than 5%	827:851	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	1	48	theme	increasing	219:228	arg1	amounts					230:236	increasing amounts	219:236	increasing amounts of attention	219:249	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	1	48	theme	increasing	219:228	arg1	attention					241:249	attention	241:249	attention	241:249	Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention.					
31362158	0	49	theme	rice	71:74	arg1	screening					39:47	preliminary screening	27:47	preliminary screening of high dietary fiber rice	27:74	High-throughput method for preliminary screening of high dietary fiber rice.					
31362158	4	50	dep	had	739:741	arg1	all					735:737	all	735:737	all	735:737	The individuals identified from the F2 population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%.					
31362158	0	51	theme	fiber	65:69	arg1	rice					71:74	high dietary fiber rice	52:74	high dietary fiber rice	52:74	High-throughput method for preliminary screening of high dietary fiber rice.					
31362158	5	52	theme	throughput	1048:1057	arg1	method					1059:1064	a simplified, economical and high throughput method	1014:1064	a simplified, economical and high throughput method for high TDF rice breeding	1014:1091	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31362158	5	53	theme	higher	944:949	arg1	content					955:961	higher TDF content	944:961	higher TDF content	944:961	The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.					
31352046	6	0	from	analysis	815:822	arg1	Data					780:783	Data	780:783	Data from ATR-FTIR, Raman, and DSC analysis	780:822	Data from ATR-FTIR, Raman, and DSC analysis confirmed drug compatibility with chitosan glutamate after spray-drying.					
31352046	5	1	theme	variables	734:742	arg1	influence					689:697	the influence	685:697	the influence of product composition and process variables on final microbeads characteristic	685:777	As polymer properties may alter upon processing, particularly important was to examine the influence of product composition and process variables on final microbeads characteristic.					
31352046	5	2	theme	final	747:751	arg1	microbeads					753:762	final microbeads	747:762	final microbeads characteristic	747:777	As polymer properties may alter upon processing, particularly important was to examine the influence of product composition and process variables on final microbeads characteristic.					
31352046	2	3	theme	polymer-based	299:311	arg1	carriers					322:329	smart polymer-based delivery carriers	293:329	smart polymer-based delivery carriers	293:329	In order to improve efficacy and accessibility of vaginal microbicides, development of smart polymer-based delivery carriers appears essential.					
31352046	6	4	from	Raman	800:804	arg1	Data					780:783	Data	780:783	Data from ATR-FTIR, Raman, and DSC analysis	780:822	Data from ATR-FTIR, Raman, and DSC analysis confirmed drug compatibility with chitosan glutamate after spray-drying.					
31352046	5	5	theme	product	702:708	arg1	composition					710:720	product composition	702:720	product composition	702:720	As polymer properties may alter upon processing, particularly important was to examine the influence of product composition and process variables on final microbeads characteristic.					
31352046	11	6	theme	mucosal	1677:1683	arg1	cells					1685:1689	mucosal cells	1677:1689	mucosal cells	1677:1689	Cytotoxicity studies using human vaginal cell line VK2/E6E7 revealed that drug-free formulations exerted no significant impact on mucosal cells, suggesting they are safe for vaginal delivery.					
31352046	1	7	theme	physicochemical	173:187	arg1	characteristics					189:203	the in vitro functional and physicochemical characteristics	145:203	the in vitro functional and physicochemical characteristics	145:203	The effect of process variables on the in vitro functional and physicochemical characteristics.					
31352046	10	8	theme	swelling	1497:1504	arg1	capacity					1506:1513	microbeads' swelling capacity	1485:1513	microbeads' swelling capacity	1485:1513	By modifying spray-drying temperature, alterations in microbeads' swelling capacity and drug release were observed.					
31352046	2	9	theme	carriers	322:329	arg1	development					278:288	development	278:288	development of smart polymer-based delivery carriers	278:329	In order to improve efficacy and accessibility of vaginal microbicides, development of smart polymer-based delivery carriers appears essential.					
31352046	6	10	theme	DSC	811:813	arg1	analysis					815:822	ATR-FTIR, Raman, and DSC analysis	790:822	analysis	815:822	Data from ATR-FTIR, Raman, and DSC analysis confirmed drug compatibility with chitosan glutamate after spray-drying.					
31352046	9	11	theme	release	1416:1422	arg1	stage					1424:1428	prolonged (up to 4 h) drug release stage	1389:1428	prolonged (up to 4 h) drug release stage	1389:1428	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	5	12	theme	characteristic	764:777	arg1	microbeads					753:762	final microbeads	747:762	final microbeads characteristic	747:777	As polymer properties may alter upon processing, particularly important was to examine the influence of product composition and process variables on final microbeads characteristic.					
31352046	1	13	theme	process	124:130	arg1	variables					132:140	process variables	124:140	process variables	124:140	The effect of process variables on the in vitro functional and physicochemical characteristics.					
31352046	7	14	theme	direct	1122:1127	arg1	contact					1129:1135	direct contact	1122:1135	direct contact of virus with mucus cells	1122:1161	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	9	15	theme	burst	1362:1366	arg1	effect					1368:1373	an initial burst effect	1351:1373	an initial burst effect	1351:1373	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	11	16	theme	vaginal	1580:1586	arg1	line					1593:1596	human vaginal cell line VK2/E6E7	1574:1605	human vaginal cell line VK2/E6E7	1574:1605	Cytotoxicity studies using human vaginal cell line VK2/E6E7 revealed that drug-free formulations exerted no significant impact on mucosal cells, suggesting they are safe for vaginal delivery.					
31352046	2	17	theme	vaginal	256:262	arg1	microbicides					264:275	vaginal microbicides	256:275	vaginal microbicides	256:275	In order to improve efficacy and accessibility of vaginal microbicides, development of smart polymer-based delivery carriers appears essential.					
31352046	5	18	theme	polymer	601:607	arg1	properties					609:618	polymer properties	601:618	polymer properties	601:618	As polymer properties may alter upon processing, particularly important was to examine the influence of product composition and process variables on final microbeads characteristic.					
31352046	5	19	from	influence	689:697	arg1	microbeads					753:762	final microbeads	747:762	final microbeads characteristic	747:777	As polymer properties may alter upon processing, particularly important was to examine the influence of product composition and process variables on final microbeads characteristic.					
31352046	4	20	theme	statistical	563:573	arg1	design					575:580	statistical design	563:580	statistical design of experiments	563:595	Spray-drying optimization was supported by statistical design of experiments.					
31352046	11	21	theme	drug-free	1621:1629	arg1	formulations					1631:1642	drug-free formulations	1621:1642	drug-free formulations	1621:1642	Cytotoxicity studies using human vaginal cell line VK2/E6E7 revealed that drug-free formulations exerted no significant impact on mucosal cells, suggesting they are safe for vaginal delivery.					
31352046	0	22	theme	vaginal	80:86	arg1	microbeads					18:27	spray-dried microbeads	6:27	spray-dried microbeads with chitosan glutamate	6:51	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	0	22	theme	vaginal	80:86	arg1	carriers					100:107	promising vaginal microbicide carriers	70:107	promising vaginal microbicide carriers	70:107	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	9	23	theme	prolonged	1389:1397	arg1	stage					1424:1428	prolonged (up to 4 h) drug release stage	1389:1428	prolonged (up to 4 h) drug release stage	1389:1428	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	1	24	theme	functional	158:167	arg1	characteristics					189:203	the in vitro functional and physicochemical characteristics	145:203	the in vitro functional and physicochemical characteristics	145:203	The effect of process variables on the in vitro functional and physicochemical characteristics.					
31352046	7	25	theme	simulant	1038:1045	arg1	fluid					1055:1059	simulant vaginal fluid	1038:1059	simulant vaginal fluid	1038:1059	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	3	26	theme	study	367:371	arg1	scope					353:357	scope	353:357	scope of this study	353:371	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	3	27	theme	zidovudine-loaded	473:489	arg1	microbeads					491:500	zidovudine-loaded microbeads	473:500	zidovudine-loaded microbeads	473:500	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	3	28	theme	glutamate	400:408	arg1	potential					378:386	the potential	374:386	the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads	374:500	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	3	29	theme	formulations	449:460	arg1	technology					413:422	technology	413:422	technology of microbicide multiunit formulations containing zidovudine-loaded microbeads	413:500	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	9	30	theme	drug	1411:1414	arg1	stage					1424:1428	prolonged (up to 4 h) drug release stage	1389:1428	prolonged (up to 4 h) drug release stage	1389:1428	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	7	31	dep	viscous	1070:1076	arg1	shear-thinning					1079:1092	shear-thinning	1079:1092	shear-thinning	1079:1092	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	3	32	theme	microbicide	427:437	arg1	formulations					449:460	microbicide multiunit formulations	427:460	microbicide multiunit formulations containing zidovudine-loaded microbeads	427:500	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	11	33	theme	Cytotoxicity	1547:1558	arg1	studies					1560:1566	Cytotoxicity studies	1547:1566	Cytotoxicity studies using human vaginal cell line VK2/E6E7	1547:1605	Cytotoxicity studies using human vaginal cell line VK2/E6E7 revealed that drug-free formulations exerted no significant impact on mucosal cells, suggesting they are safe for vaginal delivery.					
31352046	0	34	theme	chitosan	34:41	arg1	glutamate					43:51	chitosan glutamate	34:51	chitosan glutamate	34:51	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	0	35	with	microbeads	18:27	arg1	glutamate					43:51	chitosan glutamate	34:51	chitosan glutamate	34:51	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	1	36	from	effect	114:119	arg1	characteristics					189:203	the in vitro functional and physicochemical characteristics	145:203	the in vitro functional and physicochemical characteristics	145:203	The effect of process variables on the in vitro functional and physicochemical characteristics.					
31352046	8	37	theme	ethanol	1193:1199	arg1	presence					1181:1188	the presence	1177:1188	the presence of ethanol	1177:1199	Furthermore, the presence of ethanol was found crucial to overcome stickiness phenomenon by interrupting hydrogen bonding between drug and polymer.					
31352046	1	38	theme	in	149:150	arg1	characteristics					189:203	the in vitro functional and physicochemical characteristics	145:203	the in vitro functional and physicochemical characteristics	145:203	The effect of process variables on the in vitro functional and physicochemical characteristics.					
31352046	9	39	dep	displayed	1341:1349	arg1	followed					1375:1382	followed	1375:1382	followed with prolonged (up to 4 h) drug release stage	1375:1428	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	10	40	theme	spray-drying	1444:1455	arg1	temperature					1457:1467	spray-drying temperature	1444:1467	spray-drying temperature	1444:1467	By modifying spray-drying temperature, alterations in microbeads' swelling capacity and drug release were observed.					
31352046	5	41	theme	process	726:732	arg1	variables					734:742	process variables	726:742	process variables	726:742	As polymer properties may alter upon processing, particularly important was to examine the influence of product composition and process variables on final microbeads characteristic.					
31352046	11	42	theme	significant	1655:1665	arg1	impact					1667:1672	no significant impact	1652:1672	no significant impact	1652:1672	Cytotoxicity studies using human vaginal cell line VK2/E6E7 revealed that drug-free formulations exerted no significant impact on mucosal cells, suggesting they are safe for vaginal delivery.					
31352046	2	43	theme	delivery	313:320	arg1	carriers					322:329	smart polymer-based delivery carriers	293:329	smart polymer-based delivery carriers	293:329	In order to improve efficacy and accessibility of vaginal microbicides, development of smart polymer-based delivery carriers appears essential.					
31352046	7	44	theme	azeotropic	958:967	arg1	mixture					983:989	azeotropic ethanol-water mixture	958:989	azeotropic ethanol-water mixture	958:989	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	8	45	theme	stickiness	1231:1240	arg1	phenomenon					1242:1251	stickiness phenomenon	1231:1251	stickiness phenomenon	1231:1251	Furthermore, the presence of ethanol was found crucial to overcome stickiness phenomenon by interrupting hydrogen bonding between drug and polymer.					
31352046	2	46	theme	smart	293:297	arg1	carriers					322:329	smart polymer-based delivery carriers	293:329	smart polymer-based delivery carriers	293:329	In order to improve efficacy and accessibility of vaginal microbicides, development of smart polymer-based delivery carriers appears essential.					
31352046	5	47	theme	composition	710:720	arg1	influence					689:697	the influence	685:697	the influence of product composition and process variables on final microbeads characteristic	685:777	As polymer properties may alter upon processing, particularly important was to examine the influence of product composition and process variables on final microbeads characteristic.					
31352046	7	48	with	Formulations	897:908	arg1	ratio					928:932	polymer:drug ratio 5:1	915:936	polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture	915:989	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	7	48	with	Formulations	897:908	arg1	w/w					939:941	w/w	939:941	w/w	939:941	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	8	49	theme	hydrogen	1269:1276	arg1	bonding					1278:1284	hydrogen bonding	1269:1284	hydrogen bonding between drug and polymer	1269:1309	Furthermore, the presence of ethanol was found crucial to overcome stickiness phenomenon by interrupting hydrogen bonding between drug and polymer.					
31352046	10	50	from	release	1524:1530	arg1	capacity					1506:1513	microbeads' swelling capacity	1485:1513	microbeads' swelling capacity	1485:1513	By modifying spray-drying temperature, alterations in microbeads' swelling capacity and drug release were observed.					
31352046	7	51	theme	mucus	1151:1155	arg1	cells					1157:1161	mucus cells	1151:1161	mucus cells	1151:1161	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	6	52	theme	chitosan	858:865	arg1	glutamate					867:875	chitosan glutamate	858:875	chitosan glutamate	858:875	Data from ATR-FTIR, Raman, and DSC analysis confirmed drug compatibility with chitosan glutamate after spray-drying.					
31352046	7	53	with	dilution	1024:1031	arg1	fluid					1055:1059	simulant vaginal fluid	1038:1059	simulant vaginal fluid	1038:1059	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	7	54	theme	virus	1140:1144	arg1	contact					1129:1135	direct contact	1122:1135	direct contact of virus with mucus cells	1122:1161	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	9	55	theme	dissolution	1321:1331	arg1	studies					1333:1339	In vitro dissolution studies	1312:1339	In vitro dissolution studies	1312:1339	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	7	56	theme	drug	923:926	arg1	ratio					928:932	polymer:drug ratio 5:1	915:936	polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture	915:989	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	7	56	theme	drug	923:926	arg1	w/w					939:941	w/w	939:941	w/w	939:941	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	4	57	theme	Spray-drying	520:531	arg1	optimization					533:544	Spray-drying optimization	520:544	Spray-drying optimization	520:544	Spray-drying optimization was supported by statistical design of experiments.					
31352046	3	58	from	potential	378:386	arg1	technology					413:422	technology	413:422	technology of microbicide multiunit formulations containing zidovudine-loaded microbeads	413:500	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	3	59	contain	containing	462:471	arg1	formulations					449:460	microbicide multiunit formulations	427:460	microbicide multiunit formulations containing zidovudine-loaded microbeads	427:500	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	3	59	contain	containing	462:471	arg2	microbeads					491:500	zidovudine-loaded microbeads	473:500	zidovudine-loaded microbeads	473:500	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	11	60	theme	cell	1588:1591	arg1	line					1593:1596	human vaginal cell line VK2/E6E7	1574:1605	human vaginal cell line VK2/E6E7	1574:1605	Cytotoxicity studies using human vaginal cell line VK2/E6E7 revealed that drug-free formulations exerted no significant impact on mucosal cells, suggesting they are safe for vaginal delivery.					
31352046	9	61	theme	In	1312:1313	arg1	studies					1333:1339	In vitro dissolution studies	1312:1339	In vitro dissolution studies	1312:1339	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	9	62	theme	initial	1354:1360	arg1	effect					1368:1373	an initial burst effect	1351:1373	an initial burst effect	1351:1373	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	1	63	theme	variables	132:140	arg1	effect					114:119	The effect	110:119	The effect of process variables on the in vitro functional and physicochemical characteristics.	110:204	The effect of process variables on the in vitro functional and physicochemical characteristics.					
31352046	11	64	theme	human	1574:1578	arg1	line					1593:1596	human vaginal cell line VK2/E6E7	1574:1605	human vaginal cell line VK2/E6E7	1574:1605	Cytotoxicity studies using human vaginal cell line VK2/E6E7 revealed that drug-free formulations exerted no significant impact on mucosal cells, suggesting they are safe for vaginal delivery.					
31352046	0	65	theme	promising	70:78	arg1	microbeads					18:27	spray-dried microbeads	6:27	spray-dried microbeads with chitosan glutamate	6:51	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	0	65	theme	promising	70:78	arg1	carriers					100:107	promising vaginal microbicide carriers	70:107	promising vaginal microbicide carriers	70:107	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	2	66	theme	microbicides	264:275	arg1	accessibility					239:251	accessibility	239:251	accessibility	239:251	In order to improve efficacy and accessibility of vaginal microbicides, development of smart polymer-based delivery carriers appears essential.					
31352046	2	66	theme	microbicides	264:275	arg1	efficacy					226:233	efficacy	226:233	efficacy	226:233	In order to improve efficacy and accessibility of vaginal microbicides, development of smart polymer-based delivery carriers appears essential.					
31352046	0	67	theme	microbicide	88:98	arg1	microbeads					18:27	spray-dried microbeads	6:27	spray-dried microbeads with chitosan glutamate	6:51	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	0	67	theme	microbicide	88:98	arg1	carriers					100:107	promising vaginal microbicide carriers	70:107	promising vaginal microbicide carriers	70:107	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	7	68	theme	viscous	1070:1076	arg1	barrier					1094:1100	viscous, shear-thinning barrier	1070:1100	viscous, shear-thinning barrier	1070:1100	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	9	69	dep	In	1312:1313	arg1	vitro					1315:1319	vitro	1315:1319	vitro	1315:1319	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	4	70	theme	experiments	585:595	arg1	design					575:580	statistical design	563:580	statistical design of experiments	563:595	Spray-drying optimization was supported by statistical design of experiments.					
31352046	10	71	theme	drug	1519:1522	arg1	release					1524:1530	drug release	1519:1530	drug release	1519:1530	By modifying spray-drying temperature, alterations in microbeads' swelling capacity and drug release were observed.					
31352046	7	72	theme	vaginal	1047:1053	arg1	fluid					1055:1059	simulant vaginal fluid	1038:1059	simulant vaginal fluid	1038:1059	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	6	73	theme	ATR-FTIR	790:797	arg1	Raman					800:804	ATR-FTIR, Raman, and DSC analysis	790:822	Raman	800:804	Data from ATR-FTIR, Raman, and DSC analysis confirmed drug compatibility with chitosan glutamate after spray-drying.					
31352046	0	74	theme	spray-dried	6:16	arg1	microbeads					18:27	spray-dried microbeads	6:27	spray-dried microbeads with chitosan glutamate	6:51	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	0	74	theme	spray-dried	6:16	arg1	carriers					100:107	promising vaginal microbicide carriers	70:107	promising vaginal microbicide carriers	70:107	Could spray-dried microbeads with chitosan glutamate be considered as promising vaginal microbicide carriers?					
31352046	7	75	with	contact	1129:1135	arg1	cells					1157:1161	mucus cells	1151:1161	mucus cells	1151:1161	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	9	76	dep	prolonged	1389:1397	arg1	4 h					1406:1408	4 h	1406:1408	4 h	1406:1408	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	7	77	theme	polymer	915:921	arg1	ratio					928:932	polymer:drug ratio 5:1	915:936	polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture	915:989	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	7	77	theme	polymer	915:921	arg1	w/w					939:941	w/w	939:941	w/w	939:941	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31352046	3	78	theme	chitosan	391:398	arg1	glutamate					400:408	chitosan glutamate	391:408	chitosan glutamate	391:408	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	1	79	dep	in	149:150	arg1	vitro					152:156	vitro	152:156	vitro	152:156	The effect of process variables on the in vitro functional and physicochemical characteristics.					
31352046	3	80	theme	multiunit	439:447	arg1	formulations					449:460	microbicide multiunit formulations	427:460	microbicide multiunit formulations containing zidovudine-loaded microbeads	427:500	In scope of this study, the potential of chitosan glutamate in technology of microbicide multiunit formulations containing zidovudine-loaded microbeads was investigated.					
31352046	9	81	dep	4 h	1406:1408	arg1	to					1403:1404	to	1403:1404	to	1403:1404	In vitro dissolution studies displayed an initial burst effect followed with prolonged (up to 4 h) drug release stage.					
31352046	10	82	from	alterations	1470:1480	arg1	capacity					1506:1513	microbeads' swelling capacity	1485:1513	microbeads' swelling capacity	1485:1513	By modifying spray-drying temperature, alterations in microbeads' swelling capacity and drug release were observed.					
31352046	6	83	theme	drug	834:837	arg1	compatibility					839:851	drug compatibility	834:851	drug compatibility	834:851	Data from ATR-FTIR, Raman, and DSC analysis confirmed drug compatibility with chitosan glutamate after spray-drying.					
31352046	11	84	theme	vaginal	1721:1727	arg1	delivery					1729:1736	vaginal delivery	1721:1736	vaginal delivery	1721:1736	Cytotoxicity studies using human vaginal cell line VK2/E6E7 revealed that drug-free formulations exerted no significant impact on mucosal cells, suggesting they are safe for vaginal delivery.					
31352046	7	85	theme	ethanol-water	969:981	arg1	mixture					983:989	azeotropic ethanol-water mixture	958:989	azeotropic ethanol-water mixture	958:989	Formulations with polymer:drug ratio 5:1 (w/w) prepared from azeotropic ethanol-water mixture were found to spread easily upon dilution with simulant vaginal fluid, forming viscous, shear-thinning barrier, which could impede direct contact of virus with mucus cells.					
31565939	0	0	theme	Relationship	89:100	arg1	Studies					102:108	Structure-Immunogenicity Relationship Studies	64:108	Structure-Immunogenicity Relationship Studies of Lewis Y Antigen	64:127	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	1	1	theme	key	351:353	arg1	intermediates					378:390	the key tetra-/pentasaccharide intermediates	347:390	the key tetra-/pentasaccharide intermediates	347:390	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	1	2	theme	Y	167:167	arg1	antigen					175:181	cancer-associated Lewis Y (Ley) antigen	143:181	cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end	143:225	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	0	3	theme	Structure-Immunogenicity	64:87	arg1	Studies					102:108	Structure-Immunogenicity Relationship Studies	64:108	Structure-Immunogenicity Relationship Studies of Lewis Y Antigen	64:127	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	1	4	theme	tetra-/pentasaccharide	355:376	arg1	intermediates					378:390	the key tetra-/pentasaccharide intermediates	347:390	the key tetra-/pentasaccharide intermediates	347:390	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	5	5	theme	functional	973:982	arg1	vaccines					1001:1008	functional conjugate cancer vaccines	973:1008	functional conjugate cancer vaccines	973:1008	These results suggested that the short Lewis Y analogue may be utilized to develop functional conjugate cancer vaccines.					
31565939	3	6	theme	glycan-protein	570:583	arg1	conjugates					585:594	The resultant glycan-protein conjugates	556:594	The resultant glycan-protein conjugates	556:594	The resultant glycan-protein conjugates were subjected to immunological studies in mice.					
31565939	6	7	theme	reducing-end	1062:1073	arg1	residue					1083:1089	the reducing-end glucose residue	1058:1089	the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y	1058:1131	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	6	8	contain	have	1222:1225	arg2	impact					1235:1240	a broad impact	1227:1240	a broad impact	1227:1240	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	6	8	contain	have	1222:1225	arg1	discovery					1208:1216	discovery	1208:1216	discovery	1208:1216	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	3	9	theme	immunological	614:626	arg1	studies					628:634	immunological studies	614:634	immunological studies in mice	614:642	The resultant glycan-protein conjugates were subjected to immunological studies in mice.					
31565939	3	10	from	studies	628:634	arg1	mice					639:642	mice	639:642	mice	639:642	The resultant glycan-protein conjugates were subjected to immunological studies in mice.					
31565939	6	11	theme	glucose	1075:1081	arg1	residue					1083:1089	the reducing-end glucose residue	1058:1089	the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y	1058:1131	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	6	12	theme	broad	1229:1233	arg1	impact					1235:1240	a broad impact	1227:1240	a broad impact	1227:1240	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	4	13	theme	conjugates	820:829	arg1	antisera					803:810	the antisera	799:810	the antisera of both conjugates	799:829	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	3	14	theme	resultant	560:568	arg1	conjugates					585:594	The resultant glycan-protein conjugates	556:594	The resultant glycan-protein conjugates	556:594	The resultant glycan-protein conjugates were subjected to immunological studies in mice.					
31565939	1	15	theme	efficient	256:264	arg1	strategy					266:273	a highly efficient strategy	247:273	a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation	247:442	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	0	16	theme	Y	119:119	arg1	Antigen					121:127	Lewis Y Antigen	113:127	Lewis Y Antigen	113:127	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	4	17	theme	Y	722:722	arg1	antigen					724:730	Lewis Y antigen	716:730	Lewis Y antigen	716:730	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	5	18	theme	short	923:927	arg1	analogue					937:944	the short Lewis Y analogue	919:944	the short Lewis Y analogue	919:944	These results suggested that the short Lewis Y analogue may be utilized to develop functional conjugate cancer vaccines.					
31565939	0	19	theme	Lewis	113:117	arg1	Antigen					121:127	Lewis Y Antigen	113:127	Lewis Y Antigen	113:127	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	4	20	theme	antigen	724:730	arg1	conjugate					671:679	the conjugate	667:679	the conjugate of the pentasaccharide analogue of Lewis Y antigen	667:730	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	4	20	theme	antigen	724:730	arg1	immunogenic					741:751	immunogenic	741:751	immunogenic	741:751	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	2	21	theme	limpet	537:542	arg1	hemocyanin					544:553	carrier protein keyhole limpet hemocyanin	513:553	carrier protein keyhole limpet hemocyanin	513:553	After global deprotection, these oligosaccharides were coupled with carrier protein keyhole limpet hemocyanin.					
31565939	5	22	used	utilized	953:960	arg2	analogue					937:944	the short Lewis Y analogue	919:944	the short Lewis Y analogue	919:944	These results suggested that the short Lewis Y analogue may be utilized to develop functional conjugate cancer vaccines.					
31565939	6	23	with	interaction	1166:1176	arg1	system					1194:1199	the immune system	1183:1199	the immune system	1183:1199	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	1	24	theme	antigen	175:181	arg1	Analogues					130:138	Analogues	130:138	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end	130:225	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	6	25	from	residue	1083:1089	arg1	analogue					1113:1120	the hexasaccharide analogue	1094:1120	the hexasaccharide analogue of Lewis Y	1094:1131	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	0	26	theme	Y	19:19	arg1	Analogues					21:29	Lewis Y Analogues	13:29	Lewis Y Analogues	13:29	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	5	27	theme	Lewis	929:933	arg1	analogue					937:944	the short Lewis Y analogue	919:944	the short Lewis Y analogue	919:944	These results suggested that the short Lewis Y analogue may be utilized to develop functional conjugate cancer vaccines.					
31565939	6	28	theme	Y	1131:1131	arg1	analogue					1113:1120	the hexasaccharide analogue	1094:1120	the hexasaccharide analogue of Lewis Y	1094:1131	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	1	29	theme	varying	188:194	arg1	structures					196:205	varying structures	188:205	varying structures at the reducing end	188:225	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	0	30	theme	Lewis	13:17	arg1	Analogues					21:29	Lewis Y Analogues	13:29	Lewis Y Analogues	13:29	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	1	31	theme	one-pot	285:291	arg1	glycosylation					323:335	one-pot preactivation-based iterative glycosylation	285:335	one-pot preactivation-based iterative glycosylation	285:335	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	0	32	theme	Antigen	121:127	arg1	Studies					102:108	Structure-Immunogenicity Relationship Studies	64:108	Structure-Immunogenicity Relationship Studies of Lewis Y Antigen	64:127	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	5	33	theme	conjugate	984:992	arg1	vaccines					1001:1008	functional conjugate cancer vaccines	973:1008	functional conjugate cancer vaccines	973:1008	These results suggested that the short Lewis Y analogue may be utilized to develop functional conjugate cancer vaccines.					
31565939	2	34	theme	carrier	513:519	arg1	hemocyanin					544:553	carrier protein keyhole limpet hemocyanin	513:553	carrier protein keyhole limpet hemocyanin	513:553	After global deprotection, these oligosaccharides were coupled with carrier protein keyhole limpet hemocyanin.					
31565939	4	35	theme	analogue	704:711	arg1	conjugate					671:679	the conjugate	667:679	the conjugate of the pentasaccharide analogue of Lewis Y antigen	667:730	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	4	35	theme	analogue	704:711	arg1	immunogenic					741:751	immunogenic	741:751	immunogenic	741:751	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	2	36	theme	keyhole	529:535	arg1	hemocyanin					544:553	carrier protein keyhole limpet hemocyanin	513:553	carrier protein keyhole limpet hemocyanin	513:553	After global deprotection, these oligosaccharides were coupled with carrier protein keyhole limpet hemocyanin.					
31565939	1	37	theme	preactivation-based	293:311	arg1	glycosylation					323:335	one-pot preactivation-based iterative glycosylation	285:335	one-pot preactivation-based iterative glycosylation	285:335	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	1	38	theme	stereoselective	415:429	arg1	fucosylation					431:442	stereoselective fucosylation	415:442	stereoselective fucosylation	415:442	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	2	39	theme	global	451:456	arg1	deprotection					458:469	global deprotection	451:469	global deprotection	451:469	After global deprotection, these oligosaccharides were coupled with carrier protein keyhole limpet hemocyanin.					
31565939	0	40	theme	Analogues	21:29	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.	0:128	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	1	41	theme	iterative	313:321	arg1	glycosylation					323:335	one-pot preactivation-based iterative glycosylation	285:335	one-pot preactivation-based iterative glycosylation	285:335	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	2	42	theme	protein	521:527	arg1	hemocyanin					544:553	carrier protein keyhole limpet hemocyanin	513:553	carrier protein keyhole limpet hemocyanin	513:553	After global deprotection, these oligosaccharides were coupled with carrier protein keyhole limpet hemocyanin.					
31565939	6	43	theme	hexasaccharide	1098:1111	arg1	analogue					1113:1120	the hexasaccharide analogue	1094:1120	the hexasaccharide analogue of Lewis Y	1094:1131	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	4	44	theme	Lewis	716:720	arg1	antigen					724:730	Lewis Y antigen	716:730	Lewis Y antigen	716:730	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	0	45	theme	Protein	41:47	arg1	Conjugates					49:58	Their Protein Conjugates	35:58	Their Protein Conjugates	35:58	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	1	46	with	antigen	175:181	arg1	structures					196:205	varying structures	188:205	varying structures at the reducing end	188:225	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	6	47	theme	immune	1187:1192	arg1	system					1194:1199	the immune system	1183:1199	the immune system	1183:1199	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	5	48	theme	cancer	994:999	arg1	vaccines					1001:1008	functional conjugate cancer vaccines	973:1008	functional conjugate cancer vaccines	973:1008	These results suggested that the short Lewis Y analogue may be utilized to develop functional conjugate cancer vaccines.					
31565939	6	49	theme	Lewis	1125:1129	arg1	Y					1131:1131	Lewis Y	1125:1131	Lewis Y	1125:1131	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	4	50	theme	hexasaccharide	770:783	arg1	analogue					785:792	the hexasaccharide analogue	766:792	the hexasaccharide analogue	766:792	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	6	51	theme	vaccines	1270:1277	arg1	design					1249:1254	the design	1245:1254	the design of new cancer vaccines	1245:1277	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	4	52	theme	carbohydrate	869:880	arg1	hapten					882:887	each carbohydrate hapten	864:887	each carbohydrate hapten	864:887	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	1	53	theme	reducing	214:221	arg1	end					223:225	the reducing end	210:225	the reducing end	210:225	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	1	54	from	end	223:225	arg1	structures					196:205	varying structures	188:205	varying structures at the reducing end	188:225	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	6	55	theme	cancer	1263:1268	arg1	vaccines					1270:1277	new cancer vaccines	1259:1277	new cancer vaccines	1259:1277	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	5	56	theme	Y	935:935	arg1	analogue					937:944	the short Lewis Y analogue	919:944	the short Lewis Y analogue	919:944	These results suggested that the short Lewis Y analogue may be utilized to develop functional conjugate cancer vaccines.					
31565939	6	57	theme	new	1259:1261	arg1	vaccines					1270:1277	new cancer vaccines	1259:1277	new cancer vaccines	1259:1277	More importantly, the results also proved that the reducing-end glucose residue in the hexasaccharide analogue of Lewis Y was probably not involved in its interaction with the immune system, whose discovery can have a broad impact on the design of new cancer vaccines.					
31565939	1	58	theme	cancer-associated	143:159	arg1	antigen					175:181	cancer-associated Lewis Y (Ley) antigen	143:181	cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end	143:225	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	4	59	theme	pentasaccharide	688:702	arg1	analogue					704:711	the pentasaccharide analogue	684:711	the pentasaccharide analogue	684:711	It was disclosed that the conjugate of the pentasaccharide analogue of Lewis Y antigen was more immunogenic than that of the hexasaccharide analogue, but the antisera of both conjugates could indiscriminately recognize each carbohydrate hapten.					
31565939	0	60	theme	Conjugates	49:58	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.	0:128	Synthesis of Lewis Y Analogues and Their Protein Conjugates for Structure-Immunogenicity Relationship Studies of Lewis Y Antigen.					
31565939	1	61	theme	Lewis	161:165	arg1	Ley					170:172	Ley	170:172	Ley	170:172	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31565939	1	61	theme	Lewis	161:165	arg1	Y					167:167	Lewis Y	161:167	cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end	143:225	Analogues of cancer-associated Lewis Y (Ley) antigen with varying structures at the reducing end were synthesized by a highly efficient strategy involving one-pot preactivation-based iterative glycosylation to obtain the key tetra-/pentasaccharide intermediates, which was followed by stereoselective fucosylation.					
31529345	8	0	theme	adsorption	1078:1087	arg1	efficiency					1089:1098	adsorption efficiency	1078:1098	adsorption efficiency	1078:1098	The presence of the competing ions did not reduce the efficiency of the process and adsorption efficiency was higher than 93%.					
31529345	8	1	theme	process	1066:1072	arg1	efficiency					1048:1057	the efficiency	1044:1057	the efficiency of the process	1044:1072	The presence of the competing ions did not reduce the efficiency of the process and adsorption efficiency was higher than 93%.					
31529345	8	2	theme	competing	1014:1022	arg1	ions					1024:1027	the competing ions	1010:1027	the competing ions	1010:1027	The presence of the competing ions did not reduce the efficiency of the process and adsorption efficiency was higher than 93%.					
31529345	0	3	from	adsorption	10:19	arg1	nanocomposite					41:53	magnetic nanocomposite	32:53	magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers	32:91	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	0	3	from	adsorption	10:19	arg1	nanofibers					82:91	Fe3O4/zeolite/cellulose nanofibers	58:91	Fe3O4/zeolite/cellulose nanofibers	58:91	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	12	4	from	removal	1472:1478	arg1	wastewater					1483:1492	wastewater	1483:1492	wastewater	1483:1492	Based on these results, MZNF obtained in this work can be served as a promising candidate for Zn+2 removal in wastewater.					
31529345	3	5	from	speed	457:461	arg1	adsorbent					342:350	adsorbent	342:350	adsorbent	342:350	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	5	from	speed	457:461	arg1	ratio					333:337	The mass ratio	324:337	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed	324:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	6	from	time	378:381	arg1	adsorbent					342:350	adsorbent	342:350	adsorbent	342:350	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	6	from	time	378:381	arg1	ratio					333:337	The mass ratio	324:337	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed	324:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	7	7	theme	higher	820:825	arg1	efficiency					827:836	higher efficiency	820:836	higher efficiency	820:836	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	11	8	theme	adsorption	1348:1357	arg1	process					1359:1365	the adsorption process	1344:1365	the adsorption process	1344:1365	It was concluded that Pseudo second-order model and Langmuir models described the adsorption process well.					
31529345	3	9	from	adsorbent	342:350	arg1	dosage					394:399	adsorbent dosage	384:399	adsorbent dosage	384:399	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	9	from	adsorbent	342:350	arg1	temperature					430:440	temperature	430:440	temperature	430:440	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	9	from	adsorbent	342:350	arg1	pH					366:367	pH	366:367	pH	366:367	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	9	from	adsorbent	342:350	arg1	time					378:381	contact time	370:381	contact time	370:381	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	9	from	adsorbent	342:350	arg1	concentration					415:427	initial Zn+2 concentration	402:427	initial Zn+2 concentration	402:427	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	9	from	adsorbent	342:350	arg1	speed					457:461	agitation speed	447:461	agitation speed	447:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	6	10	theme	Rapid	673:677	arg1	adsorption					679:688	Rapid adsorption	673:688	Rapid adsorption at the first 30 min of the reaction	673:724	Rapid adsorption at the first 30 min of the reaction is one of the advantages of the prepared adsorbents.					
31529345	4	11	theme	important	545:553	arg1	role					555:558	an important role	542:558	an important role	542:558	The results showed that zeolite played an important role in the prepared nanocomposite due to its great surface area.					
31529345	4	12	theme	due	590:592	arg1	nanocomposite					576:588	the prepared nanocomposite	563:588	the prepared nanocomposite due to its great surface area	563:618	The results showed that zeolite played an important role in the prepared nanocomposite due to its great surface area.					
31529345	3	13	theme	batch	484:488	arg1	experiments					490:500	batch experiments	484:500	batch experiments	484:500	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	1	14	theme	adsorption	160:169	arg1	behavior					171:178	the adsorption behavior	156:178	the adsorption behavior of zinc onto magnetic zeolite/cellulose nanofibers (MZNF)	156:236	In this paper, the adsorption behavior of zinc onto magnetic zeolite/cellulose nanofibers (MZNF) was studied.					
31529345	3	15	theme	mass	328:331	arg1	ratio					333:337	The mass ratio	324:337	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed	324:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	0	16	theme	magnetic	32:39	arg1	nanocomposite					41:53	magnetic nanocomposite	32:53	magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers	32:91	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	0	16	theme	magnetic	32:39	arg1	nanofibers					82:91	Fe3O4/zeolite/cellulose nanofibers	58:91	Fe3O4/zeolite/cellulose nanofibers	58:91	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	3	17	from	temperature	430:440	arg1	adsorbent					342:350	adsorbent	342:350	adsorbent	342:350	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	17	from	temperature	430:440	arg1	ratio					333:337	The mass ratio	324:337	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed	324:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	4	18	theme	surface	607:613	arg1	area					615:618	its great surface area	597:618	its great surface area	597:618	The results showed that zeolite played an important role in the prepared nanocomposite due to its great surface area.					
31529345	3	19	theme	composite	355:363	arg1	pH					366:367	pH	366:367	pH	366:367	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	7	20	theme	optimum	890:896	arg1	conditions					898:907	optimum conditions	890:907	optimum conditions	890:907	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	10	21	theme	Equilibrium	1190:1200	arg1	data					1202:1205	Equilibrium data	1190:1205	Equilibrium data	1190:1205	Equilibrium data were examined by various isotherms and kinetics equations.					
31529345	4	22	theme	great	601:605	arg1	area					615:618	its great surface area	597:618	its great surface area	597:618	The results showed that zeolite played an important role in the prepared nanocomposite due to its great surface area.					
31529345	0	23	theme	Effective	0:8	arg1	adsorption					10:19	Effective adsorption	0:19	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.	0:139	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	7	24	theme	%	946:946	arg1	capacity					971:978	maximum adsorption capacity	952:978	maximum adsorption capacity of 9.45 mg/g	952:991	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	7	24	theme	%	946:946	arg1	efficiency					930:939	removal efficiency	922:939	removal efficiency of 96%	922:946	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	1	25	theme	zinc	183:186	arg1	behavior					171:178	the adsorption behavior	156:178	the adsorption behavior of zinc onto magnetic zeolite/cellulose nanofibers (MZNF)	156:236	In this paper, the adsorption behavior of zinc onto magnetic zeolite/cellulose nanofibers (MZNF) was studied.					
31529345	3	26	theme	adsorbent	342:350	arg1	ratio					333:337	The mass ratio	324:337	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed	324:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	7	27	from	temperature	801:811	arg1	increase					789:796	increase	789:796	increase at temperature	789:811	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	5	28	theme	removal	653:659	arg1	efficiency					661:670	the highest Zn+2 removal efficiency	636:670	the highest Zn+2 removal efficiency	636:670	pH 7 exhibited the highest Zn+2 removal efficiency.					
31529345	6	29	theme	advantages	740:749	arg1	one					729:731	one	729:731	one	729:731	Rapid adsorption at the first 30 min of the reaction is one of the advantages of the prepared adsorbents.					
31529345	6	29	theme	advantages	740:749	arg1	advantages					740:749	the advantages	736:749	the advantages of the prepared adsorbents	736:776	Rapid adsorption at the first 30 min of the reaction is one of the advantages of the prepared adsorbents.					
31529345	6	30	theme	prepared	758:765	arg1	adsorbents					767:776	the prepared adsorbents	754:776	the prepared adsorbents	754:776	Rapid adsorption at the first 30 min of the reaction is one of the advantages of the prepared adsorbents.					
31529345	0	31	theme	thermodynamic	120:132	arg1	study					134:138	thermodynamic study	120:138	thermodynamic study	120:138	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	9	32	theme	%	1147:1147	arg1	RSD					1136:1138	The calculated RSD	1121:1138	The calculated RSD of 1.42%	1121:1147	The calculated RSD of 1.42% exhibits the suitability of the process.					
31529345	10	33	theme	various	1224:1230	arg1	isotherms					1232:1240	various isotherms	1224:1240	various isotherms	1224:1240	Equilibrium data were examined by various isotherms and kinetics equations.					
31529345	1	34	theme	magnetic	193:200	arg1	MZNF					232:235	MZNF	232:235	MZNF	232:235	In this paper, the adsorption behavior of zinc onto magnetic zeolite/cellulose nanofibers (MZNF) was studied.					
31529345	1	34	theme	magnetic	193:200	arg1	nanofibers					220:229	magnetic zeolite/cellulose nanofibers	193:229	magnetic zeolite/cellulose nanofibers (MZNF)	193:236	In this paper, the adsorption behavior of zinc onto magnetic zeolite/cellulose nanofibers (MZNF) was studied.					
31529345	3	35	theme	contact	370:376	arg1	time					378:381	contact time	370:381	contact time	370:381	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	6	36	from	30 min	703:708	arg1	adsorption					679:688	Rapid adsorption	673:688	Rapid adsorption at the first 30 min of the reaction	673:724	Rapid adsorption at the first 30 min of the reaction is one of the advantages of the prepared adsorbents.					
31529345	1	37	theme	zeolite/cellulose	202:218	arg1	MZNF					232:235	MZNF	232:235	MZNF	232:235	In this paper, the adsorption behavior of zinc onto magnetic zeolite/cellulose nanofibers (MZNF) was studied.					
31529345	1	37	theme	zeolite/cellulose	202:218	arg1	nanofibers					220:229	magnetic zeolite/cellulose nanofibers	193:229	magnetic zeolite/cellulose nanofibers (MZNF)	193:236	In this paper, the adsorption behavior of zinc onto magnetic zeolite/cellulose nanofibers (MZNF) was studied.					
31529345	3	38	theme	adsorbent	384:392	arg1	dosage					394:399	adsorbent dosage	384:399	adsorbent dosage	384:399	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	39	theme	initial	402:408	arg1	concentration					415:427	initial Zn+2 concentration	402:427	initial Zn+2 concentration	402:427	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	11	40	theme	Pseudo	1288:1293	arg1	model					1308:1312	Pseudo second-order model	1288:1312	Pseudo second-order model	1288:1312	It was concluded that Pseudo second-order model and Langmuir models described the adsorption process well.					
31529345	11	41	theme	second-order	1295:1306	arg1	model					1308:1312	Pseudo second-order model	1288:1312	Pseudo second-order model	1288:1312	It was concluded that Pseudo second-order model and Langmuir models described the adsorption process well.					
31529345	3	42	from	ratio	333:337	arg1	dosage					394:399	adsorbent dosage	384:399	adsorbent dosage	384:399	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	42	from	ratio	333:337	arg1	temperature					430:440	temperature	430:440	temperature	430:440	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	42	from	ratio	333:337	arg1	pH					366:367	pH	366:367	pH	366:367	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	42	from	ratio	333:337	arg1	time					378:381	contact time	370:381	contact time	370:381	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	42	from	ratio	333:337	arg1	concentration					415:427	initial Zn+2 concentration	402:427	initial Zn+2 concentration	402:427	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	42	from	ratio	333:337	arg1	speed					457:461	agitation speed	447:461	agitation speed	447:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	0	43	theme	zinc	24:27	arg1	adsorption					10:19	Effective adsorption	0:19	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.	0:139	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	6	44	theme	reaction	717:724	arg1	30 min					703:708	the first 30 min	693:708	the first 30 min of the reaction	693:724	Rapid adsorption at the first 30 min of the reaction is one of the advantages of the prepared adsorbents.					
31529345	3	45	theme	Zn+2	410:413	arg1	concentration					415:427	initial Zn+2 concentration	402:427	initial Zn+2 concentration	402:427	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	7	46	theme	9.45 mg/g	983:991	arg1	capacity					971:978	maximum adsorption capacity	952:978	maximum adsorption capacity of 9.45 mg/g	952:991	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	7	46	theme	9.45 mg/g	983:991	arg1	efficiency					930:939	removal efficiency	922:939	removal efficiency of 96%	922:946	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	11	47	theme	Langmuir	1318:1325	arg1	models					1327:1332	Langmuir models	1318:1332	Langmuir models	1318:1332	It was concluded that Pseudo second-order model and Langmuir models described the adsorption process well.					
31529345	7	48	theme	adsorption	960:969	arg1	capacity					971:978	maximum adsorption capacity	952:978	maximum adsorption capacity of 9.45 mg/g	952:991	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	8	49	theme	ions	1024:1027	arg1	presence					998:1005	The presence	994:1005	The presence of the competing ions	994:1027	The presence of the competing ions did not reduce the efficiency of the process and adsorption efficiency was higher than 93%.					
31529345	3	50	from	concentration	415:427	arg1	adsorbent					342:350	adsorbent	342:350	adsorbent	342:350	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	50	from	concentration	415:427	arg1	ratio					333:337	The mass ratio	324:337	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed	324:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	51	theme	agitation	447:455	arg1	speed					457:461	agitation speed	447:461	agitation speed	447:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	5	52	theme	highest	640:646	arg1	efficiency					661:670	the highest Zn+2 removal efficiency	636:670	the highest Zn+2 removal efficiency	636:670	pH 7 exhibited the highest Zn+2 removal efficiency.					
31529345	9	53	theme	calculated	1125:1134	arg1	RSD					1136:1138	The calculated RSD	1121:1138	The calculated RSD of 1.42%	1121:1147	The calculated RSD of 1.42% exhibits the suitability of the process.					
31529345	10	54	dep	isotherms	1232:1240	arg1	equations					1255:1263	equations	1255:1263	equations	1255:1263	Equilibrium data were examined by various isotherms and kinetics equations.					
31529345	2	55	theme	VSM	310:312	arg1	analyses					314:321	VSM analyses	310:321	VSM analyses	310:321	The prepared adsorbent was characterized by SEM, FTIR, and VSM analyses.					
31529345	7	56	theme	removal	922:928	arg1	efficiency					930:939	removal efficiency	922:939	removal efficiency of 96%	922:946	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	12	57	theme	Zn+2	1467:1470	arg1	removal					1472:1478	Zn+2 removal	1467:1478	Zn+2 removal in wastewater	1467:1492	Based on these results, MZNF obtained in this work can be served as a promising candidate for Zn+2 removal in wastewater.					
31529345	5	58	theme	Zn+2	648:651	arg1	efficiency					661:670	the highest Zn+2 removal efficiency	636:670	the highest Zn+2 removal efficiency	636:670	pH 7 exhibited the highest Zn+2 removal efficiency.					
31529345	9	59	theme	process	1181:1187	arg1	suitability					1162:1172	the suitability	1158:1172	the suitability of the process	1158:1187	The calculated RSD of 1.42% exhibits the suitability of the process.					
31529345	7	60	theme	maximum	952:958	arg1	capacity					971:978	maximum adsorption capacity	952:978	maximum adsorption capacity of 9.45 mg/g	952:991	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	6	61	theme	first	697:701	arg1	30 min					703:708	the first 30 min	693:708	the first 30 min of the reaction	693:724	Rapid adsorption at the first 30 min of the reaction is one of the advantages of the prepared adsorbents.					
31529345	7	62	theme	maximum	842:848	arg1	efficiency					850:859	maximum efficiency	842:859	maximum efficiency	842:859	Moreover, increase at temperature led to higher efficiency and maximum efficiency was attained at 30 °C. Under optimum conditions, MZNF showed removal efficiency of 96% and maximum adsorption capacity of 9.45 mg/g.					
31529345	0	63	theme	nanofibers	82:91	arg1	nanocomposite					41:53	magnetic nanocomposite	32:53	magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers	32:91	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	0	63	theme	nanofibers	82:91	arg1	nanofibers					82:91	Fe3O4/zeolite/cellulose nanofibers	58:91	Fe3O4/zeolite/cellulose nanofibers	58:91	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	6	64	theme	adsorbents	767:776	arg1	advantages					740:749	the advantages	736:749	the advantages of the prepared adsorbents	736:776	Rapid adsorption at the first 30 min of the reaction is one of the advantages of the prepared adsorbents.					
31529345	0	65	dep	adsorption	10:19	arg1	equilibrium					103:113	equilibrium	103:113	equilibrium	103:113	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	0	65	dep	adsorption	10:19	arg1	kinetic					94:100	kinetic	94:100	kinetic	94:100	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	0	65	dep	adsorption	10:19	arg1	study					134:138	thermodynamic study	120:138	thermodynamic study	120:138	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	12	66	theme	promising	1443:1451	arg1	candidate					1453:1461	a promising candidate	1441:1461	a promising candidate for Zn+2 removal in wastewater	1441:1492	Based on these results, MZNF obtained in this work can be served as a promising candidate for Zn+2 removal in wastewater.					
31529345	0	67	theme	Fe3O4/zeolite/cellulose	58:80	arg1	nanofibers					82:91	Fe3O4/zeolite/cellulose nanofibers	58:91	Fe3O4/zeolite/cellulose nanofibers	58:91	Effective adsorption of zinc on magnetic nanocomposite of Fe3O4/zeolite/cellulose nanofibers: kinetic, equilibrium, and thermodynamic study.					
31529345	3	68	from	pH	366:367	arg1	adsorbent					342:350	adsorbent	342:350	adsorbent	342:350	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	68	from	pH	366:367	arg1	ratio					333:337	The mass ratio	324:337	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed	324:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	69	from	dosage	394:399	arg1	adsorbent					342:350	adsorbent	342:350	adsorbent	342:350	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	3	69	from	dosage	394:399	arg1	ratio					333:337	The mass ratio	324:337	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed	324:461	The mass ratio of adsorbent in composite, pH, contact time, adsorbent dosage, initial Zn+2 concentration, temperature, and agitation speed were investigated in batch experiments.					
31529345	4	70	theme	prepared	567:574	arg1	nanocomposite					576:588	the prepared nanocomposite	563:588	the prepared nanocomposite due to its great surface area	563:618	The results showed that zeolite played an important role in the prepared nanocomposite due to its great surface area.					
30424006	1	0	theme	microbiota	249:258	arg1	composition					260:270	intestinal microbiota composition	238:270	intestinal microbiota composition	238:270	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	2	1	theme	Thirty-one	286:295	arg1	pigs					310:313	Thirty-one six-week-old pigs	286:313	Thirty-one six-week-old pigs	286:313	Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks.					
30424006	4	2	dep	analysis	725:732	arg1	LEfSe					780:784	LEfSe	780:784	LEfSe	780:784	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	4	2	dep	analysis	725:732	arg1	size					774:777	effect size	767:777	effect size (LEfSe)	767:785	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	4	3	theme	Isolated	640:647	arg1	DNA					649:651	Isolated DNA	640:651	Isolated DNA from fecal and proximal colon contents	640:690	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	3	4	theme	alveolar	542:549	arg1	AM					564:565	AM	564:565	AM	564:565	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	3	4	theme	alveolar	542:549	arg1	macrophages					551:561	alveolar macrophages	542:561	alveolar macrophages (AM)	542:566	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	1	5	theme	dietary	187:193	arg1	mushrooms					195:203	dietary mushrooms	187:203	dietary mushrooms	187:203	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	6	6	theme	IL-1β	968:972	arg1	expression					979:988	LPS-induced IL-1β gene expression	956:988	LPS-induced IL-1β gene expression in AM (P < 0.05)	956:1005	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	7	7	with	Clostridiales	1164:1176	arg1	shift					1185:1189	a shift	1183:1189	a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs	1183:1293	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	6	8	theme	intestinal	1052:1061	arg1	mucosa					1063:1068	the intestinal mucosa	1048:1068	the intestinal mucosa	1048:1068	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	7	9	from	shift	1185:1189	arg1	metabolism					1217:1226	bacterial carbohydrate metabolism	1194:1226	bacterial carbohydrate metabolism	1194:1226	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	7	9	from	shift	1185:1189	arg1	biosynthesis					1232:1243	biosynthesis	1232:1243	biosynthesis	1232:1243	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	1	10	theme	host	212:215	arg1	response					224:231	the host immune response	208:231	the host immune response	208:231	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	6	11	theme	Feeding	930:936	arg1	mushrooms					938:946	Feeding mushrooms	930:946	Feeding mushrooms	930:946	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	7	12	from	metabolism	1217:1226	arg1	pigs					1290:1293	the mushroom-fed pigs	1273:1293	the mushroom-fed pigs	1273:1293	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	8	13	theme	taxa	1513:1516	arg1	abundance					1486:1494	the abundance	1482:1494	the abundance of Clostridiales taxa that are associated with improved intestinal health	1482:1568	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	4	14	theme	fecal	658:662	arg1	contents					683:690	fecal and proximal colon contents	658:690	fecal and proximal colon contents	658:690	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	3	15	theme	Salmonella	591:600	arg1	LPS					634:636	LPS	634:636	LPS	634:636	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	3	15	theme	Salmonella	591:600	arg1	typhymurium-Lipopolysaccharide					602:631	Salmonella typhymurium-Lipopolysaccharide	591:631	Salmonella typhymurium-Lipopolysaccharide (LPS)	591:637	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	5	16	theme	intestinal	905:914	arg1	permeability					916:927	intestinal permeability	905:927	intestinal permeability	905:927	Pigs gained weight with no difference in body composition or intestinal permeability.					
30424006	7	17	from	biosynthesis	1232:1243	arg1	pigs					1290:1293	the mushroom-fed pigs	1273:1293	the mushroom-fed pigs	1273:1293	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	8	18	theme	WB	1333:1334	arg1	mushrooms					1336:1344	feeding WB mushrooms	1325:1344	feeding WB mushrooms	1325:1344	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	3	19	with	stimulation	574:584	arg1	LPS					634:636	LPS	634:636	LPS	634:636	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	3	19	with	stimulation	574:584	arg1	typhymurium-Lipopolysaccharide					602:631	Salmonella typhymurium-Lipopolysaccharide	591:631	Salmonella typhymurium-Lipopolysaccharide (LPS)	591:637	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	6	20	theme	P	997:997	arg1	AM					993:994	AM	993:994	AM (P < 0.05)	993:1005	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	6	20	theme	P	997:997	arg1	<					999:999	P < 0.05	997:1004	P < 0.05	997:1004	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	4	21	theme	colon	677:681	arg1	contents					683:690	fecal and proximal colon contents	658:690	fecal and proximal colon contents	658:690	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	4	22	used	used	697:700	arg2	DNA					649:651	Isolated DNA	640:651	Isolated DNA from fecal and proximal colon contents	640:690	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	1	23	theme	potential	157:165	arg1	effect					177:182	the potential prebiotic effect	153:182	the potential prebiotic effect of dietary mushrooms on the host immune response	153:231	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	8	24	theme	intestinal	1552:1561	arg1	health					1563:1568	improved intestinal health	1543:1568	improved intestinal health	1543:1568	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	4	25	theme	linear	738:743	arg1	analysis					758:765	linear discriminant analysis	738:765	linear discriminant analysis	738:765	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	0	26	theme	Host	90:93	arg1	Function					109:116	Host Immunological Function	90:116	Host Immunological Function	90:116	The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function.					
30424006	8	27	theme	inflammatory	1384:1395	arg1	response					1397:1404	the LPS-induced inflammatory response	1368:1404	the LPS-induced inflammatory response	1368:1404	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	2	28	theme	-mushrooms	431:440	arg1	servings					389:396	either three or six servings	369:396	either three or six servings of freeze-dried white button (WB)-mushrooms	369:440	Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks.					
30424006	4	29	theme	rDNA	710:713	arg1	analysis					725:732	16S rDNA taxonomic analysis	706:732	16S rDNA taxonomic analysis	706:732	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	6	30	theme	LPS-stimulated	1025:1038	arg1	PBMC					1040:1043	LPS-stimulated PBMC	1025:1043	LPS-stimulated PBMC	1025:1043	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	2	31	theme	WB	428:429	arg1	-mushrooms					431:440	freeze-dried white button (WB)-mushrooms	401:440	freeze-dried white button (WB)-mushrooms	401:440	Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks.					
30424006	2	32	theme	button	420:425	arg1	-mushrooms					431:440	freeze-dried white button (WB)-mushrooms	401:440	freeze-dried white button (WB)-mushrooms	401:440	Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks.					
30424006	7	33	theme	order	1158:1162	arg1	Clostridiales					1164:1176	the order Clostridiales	1154:1176	the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs	1154:1293	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	2	34	theme	freeze-dried	401:412	arg1	-mushrooms					431:440	freeze-dried white button (WB)-mushrooms	401:440	freeze-dried white button (WB)-mushrooms	401:440	Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks.					
30424006	0	35	theme	Intestinal	52:61	arg1	Composition					74:84	Intestinal Microbiota Composition	52:84	Intestinal Microbiota Composition	52:84	The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function.					
30424006	8	36	theme	host	1441:1444	arg1	metabolism					1457:1466	the host microbiota metabolism	1437:1466	the host microbiota metabolism	1437:1466	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	0	37	theme	bisporus	40:47	arg1	Effect					4:9	The Effect	0:9	The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function.	0:117	The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function.					
30424006	2	38	theme	grower	330:335	arg1	diet					337:340	a pig grower diet	324:340	a pig grower diet alone	324:346	Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks.					
30424006	1	39	theme	immune	217:222	arg1	response					224:231	the host immune response	208:231	the host immune response	208:231	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	7	40	theme	mushroom-fed	1277:1288	arg1	pigs					1290:1293	the mushroom-fed pigs	1273:1293	the mushroom-fed pigs	1273:1293	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	6	41	dep	change	1015:1020	arg1	transcriptome					1070:1082	transcriptome	1070:1082	transcriptome	1070:1082	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	3	42	theme	peripheral	495:504	arg1	PBMC					531:534	PBMC	531:534	PBMC	531:534	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	3	42	theme	peripheral	495:504	arg1	cells					524:528	peripheral blood mononuclear cells	495:528	peripheral blood mononuclear cells (PBMC)	495:535	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	4	43	from	contents	683:690	arg1	DNA					649:651	Isolated DNA	640:651	Isolated DNA from fecal and proximal colon contents	640:690	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	1	44	theme	intestinal	238:247	arg1	composition					260:270	intestinal microbiota composition	238:270	intestinal microbiota composition	238:270	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	7	45	theme	secondary	1248:1256	arg1	metabolites					1258:1268	secondary metabolites	1248:1268	secondary metabolites in the mushroom-fed pigs	1248:1293	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	4	46	theme	bacterial	800:808	arg1	abundance					810:818	bacterial abundance	800:818	bacterial abundance	800:818	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	2	47	theme	six-week-old	297:308	arg1	pigs					310:313	Thirty-one six-week-old pigs	286:313	Thirty-one six-week-old pigs	286:313	Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks.					
30424006	7	48	from	metabolites	1258:1268	arg1	pigs					1290:1293	the mushroom-fed pigs	1273:1293	the mushroom-fed pigs	1273:1293	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	3	49	theme	mononuclear	512:522	arg1	PBMC					531:534	PBMC	531:534	PBMC	531:534	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	3	49	theme	mononuclear	512:522	arg1	cells					524:528	peripheral blood mononuclear cells	495:528	peripheral blood mononuclear cells (PBMC)	495:535	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	1	50	theme	prebiotic	167:175	arg1	effect					177:182	the potential prebiotic effect	153:182	the potential prebiotic effect of dietary mushrooms on the host immune response	153:231	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	1	51	from	function	276:283	arg1	response					224:231	the host immune response	208:231	the host immune response	208:231	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	6	52	theme	gene	974:977	arg1	expression					979:988	LPS-induced IL-1β gene expression	956:988	LPS-induced IL-1β gene expression in AM (P < 0.05)	956:1005	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	4	53	theme	metabolic	824:832	arg1	function					834:841	metabolic function	824:841	metabolic function	824:841	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	7	54	from	pigs	1290:1293	arg1	metabolism					1217:1226	bacterial carbohydrate metabolism	1194:1226	bacterial carbohydrate metabolism	1194:1226	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	7	54	from	pigs	1290:1293	arg1	biosynthesis					1232:1243	biosynthesis	1232:1243	biosynthesis	1232:1243	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	1	55	from	composition	260:270	arg1	response					224:231	the host immune response	208:231	the host immune response	208:231	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	6	56	from	change	1015:1020	arg1	PBMC					1040:1043	LPS-stimulated PBMC	1025:1043	LPS-stimulated PBMC	1025:1043	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	5	57	from	difference	871:880	arg1	composition					890:900	body composition	885:900	body composition	885:900	Pigs gained weight with no difference in body composition or intestinal permeability.					
30424006	5	57	from	difference	871:880	arg1	permeability					916:927	intestinal permeability	905:927	intestinal permeability	905:927	Pigs gained weight with no difference in body composition or intestinal permeability.					
30424006	1	58	theme	mushrooms	195:203	arg1	function					276:283	function	276:283	function	276:283	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	1	58	theme	mushrooms	195:203	arg1	composition					260:270	intestinal microbiota composition	238:270	intestinal microbiota composition	238:270	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	1	58	theme	mushrooms	195:203	arg1	effect					177:182	the potential prebiotic effect	153:182	the potential prebiotic effect of dietary mushrooms on the host immune response	153:231	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	6	59	theme	LPS-induced	956:966	arg1	expression					979:988	LPS-induced IL-1β gene expression	956:988	LPS-induced IL-1β gene expression in AM (P < 0.05)	956:1005	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	8	60	theme	Clostridiales	1499:1511	arg1	taxa					1513:1516	Clostridiales taxa	1499:1516	Clostridiales taxa that are associated with improved intestinal health	1499:1568	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	5	61	theme	body	885:888	arg1	composition					890:900	body composition	885:900	body composition	885:900	Pigs gained weight with no difference in body composition or intestinal permeability.					
30424006	3	62	theme	Host	457:460	arg1	response					469:476	Host immune response	457:476	Host immune response	457:476	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	0	63	theme	Microbiota	63:72	arg1	Composition					74:84	Intestinal Microbiota Composition	52:84	Intestinal Microbiota Composition	52:84	The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function.					
30424006	8	64	theme	improved	1543:1550	arg1	health					1563:1568	improved intestinal health	1543:1568	improved intestinal health	1543:1568	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	8	65	theme	feeding	1325:1331	arg1	mushrooms					1336:1344	feeding WB mushrooms	1325:1344	feeding WB mushrooms	1325:1344	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	7	66	theme	bacterial	1194:1202	arg1	metabolism					1217:1226	bacterial carbohydrate metabolism	1194:1226	bacterial carbohydrate metabolism	1194:1226	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	7	67	theme	carbohydrate	1204:1215	arg1	metabolism					1217:1226	bacterial carbohydrate metabolism	1194:1226	bacterial carbohydrate metabolism	1194:1226	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	1	68	from	effect	177:182	arg1	response					224:231	the host immune response	208:231	the host immune response	208:231	A study was designed to determine the potential prebiotic effect of dietary mushrooms on the host immune response, and intestinal microbiota composition and function.					
30424006	0	69	from	Effect	4:9	arg1	Composition					74:84	Intestinal Microbiota Composition	52:84	Intestinal Microbiota Composition	52:84	The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function.					
30424006	0	69	from	Effect	4:9	arg1	Function					109:116	Host Immunological Function	90:116	Host Immunological Function	90:116	The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function.					
30424006	4	70	theme	proximal	668:675	arg1	contents					683:690	fecal and proximal colon contents	658:690	fecal and proximal colon contents	658:690	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	0	71	theme	Immunological	95:107	arg1	Function					109:116	Host Immunological Function	90:116	Host Immunological Function	90:116	The Effect of Dietary Mushroom Agaricus bisporus on Intestinal Microbiota Composition and Host Immunological Function.					
30424006	8	72	theme	microbiota	1446:1455	arg1	metabolism					1457:1466	the host microbiota metabolism	1437:1466	the host microbiota metabolism	1437:1466	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	2	73	theme	pig	326:328	arg1	diet					337:340	a pig grower diet	324:340	a pig grower diet alone	324:346	Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks.					
30424006	6	74	from	expression	979:988	arg1	AM					993:994	AM	993:994	AM (P < 0.05)	993:1005	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	6	74	from	expression	979:988	arg1	<					999:999	P < 0.05	997:1004	P < 0.05	997:1004	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	4	75	theme	discriminant	745:756	arg1	analysis					758:765	linear discriminant analysis	738:765	linear discriminant analysis	738:765	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	4	76	theme	16S	706:708	arg1	analysis					725:732	16S rDNA taxonomic analysis	706:732	16S rDNA taxonomic analysis	706:732	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	4	77	theme	taxonomic	715:723	arg1	analysis					725:732	16S rDNA taxonomic analysis	706:732	16S rDNA taxonomic analysis	706:732	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	2	78	theme	white	414:418	arg1	-mushrooms					431:440	freeze-dried white button (WB)-mushrooms	401:440	freeze-dried white button (WB)-mushrooms	401:440	Thirty-one six-week-old pigs were fed a pig grower diet alone or supplemented with either three or six servings of freeze-dried white button (WB)-mushrooms for six weeks.					
30424006	7	79	from	increases	1101:1109	arg1	Ruminococcaceae					1131:1145	Ruminococcaceae	1131:1145	Ruminococcaceae	1131:1145	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	8	80	theme	LPS-induced	1372:1382	arg1	response					1397:1404	the LPS-induced inflammatory response	1368:1404	the LPS-induced inflammatory response	1368:1404	These results suggested that feeding WB mushrooms significantly reduced the LPS-induced inflammatory response in AM and positively modulated the host microbiota metabolism by increasing the abundance of Clostridiales taxa that are associated with improved intestinal health.					
30424006	4	81	theme	effect	767:772	arg1	LEfSe					780:784	LEfSe	780:784	LEfSe	780:784	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	4	81	theme	effect	767:772	arg1	size					774:777	effect size	767:777	effect size (LEfSe)	767:785	Isolated DNA from fecal and proximal colon contents were used for 16S rDNA taxonomic analysis and linear discriminant analysis effect size (LEfSe) to determine bacterial abundance and metabolic function.					
30424006	6	82	from	mucosa	1063:1068	arg1	PBMC					1040:1043	LPS-stimulated PBMC	1025:1043	LPS-stimulated PBMC	1025:1043	Feeding mushrooms reduced LPS-induced IL-1β gene expression in AM (P < 0.05) with no change in LPS-stimulated PBMC or the intestinal mucosa transcriptome.					
30424006	3	83	theme	immune	462:467	arg1	response					469:476	Host immune response	457:476	Host immune response	457:476	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	7	84	theme	metabolites	1258:1268	arg1	metabolism					1217:1226	bacterial carbohydrate metabolism	1194:1226	bacterial carbohydrate metabolism	1194:1226	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	7	84	theme	metabolites	1258:1268	arg1	biosynthesis					1232:1243	biosynthesis	1232:1243	biosynthesis	1232:1243	LEfSe indicated increases in Lachnospiraceae, Ruminococcaceae within the order Clostridiales with a shift in bacterial carbohydrate metabolism and biosynthesis of secondary metabolites in the mushroom-fed pigs.					
30424006	3	85	theme	blood	506:510	arg1	PBMC					531:534	PBMC	531:534	PBMC	531:534	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
30424006	3	85	theme	blood	506:510	arg1	cells					524:528	peripheral blood mononuclear cells	495:528	peripheral blood mononuclear cells (PBMC)	495:535	Host immune response was evaluated in peripheral blood mononuclear cells (PBMC), and alveolar macrophages (AM) after stimulation with Salmonella typhymurium-Lipopolysaccharide (LPS).					
31848088	1	0	theme	special	369:375	arg1	attention					377:385	a special attention	367:385	a special attention	367:385	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	1	0	theme	special	369:375	arg1	paid					397:400	paid	397:400	paid	397:400	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	7	1	theme	>	1740:1740	arg1	>					1747:1747	pH > dose >	1737:1747	pH > dose >	1737:1747	The results showed that the order of the variables' effectiveness is as follow: pH > dose > TC concentration.					
31848088	8	2	from	%	1827:1827	arg1	conditions					1846:1855	the optimized conditions	1832:1855	the optimized conditions by GA	1832:1861	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	4	3	theme	maximum	916:922	arg1	capacity					935:942	maximum adsorption capacity	916:942	maximum adsorption capacity	916:942	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	5	4	theme	operational	1264:1274	arg1	conditions					1276:1285	various operational conditions	1256:1285	various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time	1256:1356	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	6	5	theme	PEI-β-CD	1633:1640	arg1	nanocomposite					1642:1654	Fe3O4-g-CN@PEI-β-CD nanocomposite	1622:1654	Fe3O4-g-CN@PEI-β-CD nanocomposite	1622:1654	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	0	6	theme	regression	172:181	arg1	tree					183:186	boosted regression tree	164:186	boosted regression tree (BRT)	164:192	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	0	6	theme	regression	172:181	arg1	BRT					189:191	BRT	189:191	BRT	189:191	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	5	7	theme	network	1235:1241	arg1	approaches					1128:1137	the approaches	1124:1137	the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN)	1124:1248	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	4	8	theme	mg	994:995	arg1	g-1					997:999	833.33 mg g-1	987:999	833.33 mg g-1	987:999	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	6	9	theme	Fe3O4-g-CN	1622:1631	arg1	nanocomposite					1642:1654	Fe3O4-g-CN@PEI-β-CD nanocomposite	1622:1654	Fe3O4-g-CN@PEI-β-CD nanocomposite	1622:1654	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	7	10	theme	effectiveness	1709:1721	arg1	order					1685:1689	the order	1681:1689	the order of the variables' effectiveness	1681:1721	The results showed that the order of the variables' effectiveness is as follow: pH > dose > TC concentration.					
31848088	6	11	theme	BRT	1436:1438	arg1	model					1440:1444	the BRT model	1432:1444	the BRT model	1432:1444	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	6	11	theme	BRT	1436:1438	arg1	accurate					1455:1462	accurate	1455:1462	accurate	1455:1462	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	8	12	theme	as-prepared	1893:1903	arg1	nanocomposite					1905:1917	the as-prepared nanocomposite	1889:1917	the as-prepared nanocomposite	1889:1917	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	4	13	theme	Langmuir	964:971	arg1	model					973:977	the Langmuir model	960:977	the Langmuir model reached 833.33 mg g-1	960:999	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	6	14	theme	modeling	1596:1603	arg1	adsorption					1608:1617	modeling TC adsorption	1596:1617	modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite	1596:1654	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	4	15	dep	model	973:977	arg1	the					947:949	the	947:949	the	947:949	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	4	15	dep	model	973:977	arg1	basis					951:955	basis	951:955	basis	951:955	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	2	16	theme	@	511:511	arg1	PEI-β-CD					512:519	Fe3O4-g-CN@PEI-β-CD	501:519	Fe3O4-g-CN@PEI-β-CD	501:519	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	3	17	theme	successful	682:691	arg1	fabrication					693:703	The successful fabrication	678:703	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g)	678:771	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	0	18	theme	response	128:135	arg1	RSM					158:160	RSM	158:160	RSM	158:160	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	0	18	theme	response	128:135	arg1	methodology					145:155	response surface methodology	128:155	response surface methodology (RSM)	128:161	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	6	19	theme	good	1398:1401	arg1	predictions					1403:1413	good predictions	1398:1413	good predictions of all	1398:1420	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	6	20	theme	models	1381:1386	arg1	comparison					1363:1372	The comparison	1359:1372	The comparison of the models	1359:1386	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	8	21	theme	wastewaters	1968:1978	arg1	remediation					1931:1941	in situ remediation	1923:1941	in situ remediation of antibiotic-containing wastewaters	1923:1978	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	5	22	theme	composite	1150:1158	arg1	CCD					1168:1170	CCD	1168:1170	CCD	1168:1170	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	22	theme	composite	1150:1158	arg1	design					1160:1165	central composite design	1142:1165	central composite design (CCD)	1142:1171	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	3	23	theme	nanocomposite	712:724	arg1	fabrication					693:703	The successful fabrication	678:703	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g)	678:771	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	0	24	theme	tetracycline	43:54	arg1	adsorption					29:38	Bio-inspired, high, and fast adsorption	0:38	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media	0:73	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	4	25	theme	Fe3O4-g-CN	846:855	arg1	NC					866:867	The Fe3O4-g-CN@PEI-β-CD NC	842:867	The Fe3O4-g-CN@PEI-β-CD NC	842:867	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	0	26	theme	aqueous	61:67	arg1	media					69:73	aqueous media	61:73	aqueous media	61:73	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	3	27	theme	specific	738:745	arg1	area					755:758	a high specific surface area	731:758	a high specific surface area (57.12 m2/g)	731:771	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	3	27	theme	specific	738:745	arg1	m2/g					767:770	57.12 m2/g	761:770	57.12 m2/g	761:770	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	0	28	theme	general	199:205	arg1	GRNN					234:237	GRNN	234:237	GRNN	234:237	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	0	28	theme	general	199:205	arg1	network					225:231	general regression neural network	199:231	general regression neural network (GRNN)	199:238	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	2	29	theme	adsorptive	570:579	arg1	removal					581:587	the adsorptive removal	566:587	the adsorptive removal of tetracycline (TC)	566:608	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	5	30	theme	boosted	1174:1180	arg1	BRT					1199:1201	BRT	1199:1201	BRT	1199:1201	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	30	theme	boosted	1174:1180	arg1	tree					1193:1196	boosted regression tree	1174:1196	boosted regression tree (BRT)	1174:1202	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	0	31	theme	@	91:91	arg1	nanocomposite					101:113	Fe3O4-g-CN@PEI-β-CD nanocomposite	81:113	Fe3O4-g-CN@PEI-β-CD nanocomposite	81:113	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	0	32	theme	neural	218:223	arg1	GRNN					234:237	GRNN	234:237	GRNN	234:237	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	0	32	theme	neural	218:223	arg1	network					225:231	general regression neural network	199:231	general regression neural network (GRNN)	199:238	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	0	33	dep	using	75:79	arg1	Modeling					116:123	Modeling	116:123	Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN)	116:238	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	4	34	theme	adsorption	884:893	arg1	rates					895:899	fast adsorption rates	879:899	fast adsorption rates towards TC and maximum adsorption capacity	879:942	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	5	35	theme	TC	1298:1299	arg1	concentration					1301:1313	initial TC concentration	1290:1313	initial TC concentration	1290:1313	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	4	36	theme	carbon-	1046:1052	arg1	materials					1072:1080	different carbon- and/or nano-based materials	1036:1080	different carbon- and/or nano-based materials	1036:1080	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	5	37	theme	tree	1193:1196	arg1	approaches					1128:1137	the approaches	1124:1137	the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN)	1124:1248	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	0	38	theme	Bio-inspired	0:11	arg1	adsorption					29:38	Bio-inspired, high, and fast adsorption	0:38	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media	0:73	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	8	39	theme	adsorption	1776:1785	arg1	capacity					1787:1794	The high adsorption capacity	1767:1794	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA)	1767:1862	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	4	40	theme	nano-based	1061:1070	arg1	materials					1072:1080	different carbon- and/or nano-based materials	1036:1080	different carbon- and/or nano-based materials	1036:1080	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	0	41	theme	high	14:17	arg1	adsorption					29:38	Bio-inspired, high, and fast adsorption	0:38	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media	0:73	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	4	42	theme	PEI-β-CD	857:864	arg1	NC					866:867	The Fe3O4-g-CN@PEI-β-CD NC	842:867	The Fe3O4-g-CN@PEI-β-CD NC	842:867	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	1	43	theme	antibiotic-containing	249:269	arg1	wastewaters					271:281	antibiotic-containing wastewaters	249:281	antibiotic-containing wastewaters	249:281	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	8	44	theme	high	1807:1810	arg1	efficiency					1812:1821	high efficiency	1807:1821	high efficiency (98 % in the optimized conditions by GA)	1807:1862	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	5	45	theme	dose	1330:1333	arg1	conditions					1276:1285	various operational conditions	1256:1285	various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time	1256:1356	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	46	theme	general	1209:1215	arg1	GRNN					1244:1247	GRNN	1244:1247	GRNN	1244:1247	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	46	theme	general	1209:1215	arg1	network					1235:1241	general regression neural network	1209:1241	general regression neural network (GRNN)	1209:1248	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	6	47	dep	AAD	1539:1541	arg1	=					1543:1543	=	1543:1543	=	1543:1543	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	6	48	theme	MAE	1521:1523	arg1	0.0014					1527:1532	MAE = 0.0014	1521:1532	MAE = 0.0014	1521:1532	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	5	49	theme	tempreature	1336:1346	arg1	conditions					1276:1285	various operational conditions	1256:1285	various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time	1256:1356	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	50	theme	neural	1228:1233	arg1	GRNN					1244:1247	GRNN	1244:1247	GRNN	1244:1247	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	50	theme	neural	1228:1233	arg1	network					1235:1241	general regression neural network	1209:1241	general regression neural network (GRNN)	1209:1248	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	6	51	theme	powerful	1574:1581	arg1	it					1566:1567	it	1566:1567	it	1566:1567	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	6	51	theme	powerful	1574:1581	arg1	approach					1583:1590	a powerful approach	1572:1590	a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite	1572:1654	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	5	52	theme	adsorption	1087:1096	arg1	process					1098:1104	The adsorption process	1083:1104	The adsorption process	1083:1104	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	4	53	theme	adsorption	924:933	arg1	capacity					935:942	maximum adsorption capacity	916:942	maximum adsorption capacity	916:942	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	0	54	theme	boosted	164:170	arg1	tree					183:186	boosted regression tree	164:186	boosted regression tree (BRT)	164:192	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	0	54	theme	boosted	164:170	arg1	BRT					189:191	BRT	189:191	BRT	189:191	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	5	55	theme	various	1256:1262	arg1	conditions					1276:1285	various operational conditions	1256:1285	various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time	1256:1356	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	8	56	dep	efficiency	1812:1821	arg1	%					1827:1827	98 %	1824:1827	98 %	1824:1827	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	7	57	theme	dose	1742:1745	arg1	>					1747:1747	pH > dose >	1737:1747	pH > dose >	1737:1747	The results showed that the order of the variables' effectiveness is as follow: pH > dose > TC concentration.					
31848088	2	58	theme	PEI-β-CD	512:519	arg1	PEI-β-CD					512:519	Fe3O4-g-CN@PEI-β-CD	501:519	Fe3O4-g-CN@PEI-β-CD	501:519	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	2	58	theme	PEI-β-CD	512:519	arg1	NC					494:495	NC	494:495	NC	494:495	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	2	58	theme	PEI-β-CD	512:519	arg1	nanocomposite					479:491	the novel nanocomposite	469:491	the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD	469:519	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	4	59	theme	833.33	987:992	arg1	mg					994:995	mg	994:995	mg	994:995	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	7	60	theme	pH	1737:1738	arg1	>					1747:1747	pH > dose >	1737:1747	pH > dose >	1737:1747	The results showed that the order of the variables' effectiveness is as follow: pH > dose > TC concentration.					
31848088	6	61	theme	@	1632:1632	arg1	nanocomposite					1642:1654	Fe3O4-g-CN@PEI-β-CD nanocomposite	1622:1654	Fe3O4-g-CN@PEI-β-CD nanocomposite	1622:1654	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	1	62	theme	appropriate	416:426	arg1	materials					428:436	appropriate materials	416:436	appropriate materials for their remediation	416:458	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	0	63	from	media	69:73	arg1	adsorption					29:38	Bio-inspired, high, and fast adsorption	0:38	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media	0:73	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	6	64	theme	TC	1605:1606	arg1	adsorption					1608:1617	modeling TC adsorption	1596:1617	modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite	1596:1654	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	2	65	theme	novel	473:477	arg1	PEI-β-CD					512:519	Fe3O4-g-CN@PEI-β-CD	501:519	Fe3O4-g-CN@PEI-β-CD	501:519	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	2	65	theme	novel	473:477	arg1	NC					494:495	NC	494:495	NC	494:495	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	2	65	theme	novel	473:477	arg1	nanocomposite					479:491	the novel nanocomposite	469:491	the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD	469:519	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	8	66	theme	nanocomposite	1905:1917	arg1	potential					1876:1884	the potential	1872:1884	the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters	1872:1978	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	0	67	theme	surface	137:143	arg1	RSM					158:160	RSM	158:160	RSM	158:160	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	0	67	theme	surface	137:143	arg1	methodology					145:155	response surface methodology	128:155	response surface methodology (RSM)	128:161	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	2	68	theme	Fe3O4-g-CN	501:510	arg1	PEI-β-CD					512:519	Fe3O4-g-CN@PEI-β-CD	501:519	Fe3O4-g-CN@PEI-β-CD	501:519	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	8	69	theme	in	1923:1924	arg1	remediation					1931:1941	in situ remediation	1923:1941	in situ remediation of antibiotic-containing wastewaters	1923:1978	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	4	70	theme	different	1036:1044	arg1	materials					1072:1080	different carbon- and/or nano-based materials	1036:1080	different carbon- and/or nano-based materials	1036:1080	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	5	71	theme	central	1142:1148	arg1	CCD					1168:1170	CCD	1168:1170	CCD	1168:1170	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	71	theme	central	1142:1148	arg1	design					1160:1165	central composite design	1142:1165	central composite design (CCD)	1142:1171	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	3	72	theme	high	733:736	arg1	area					755:758	a high specific surface area	731:758	a high specific surface area (57.12 m2/g)	731:771	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	3	72	theme	high	733:736	arg1	m2/g					767:770	57.12 m2/g	761:770	57.12 m2/g	761:770	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	8	73	theme	antibiotic-containing	1946:1966	arg1	wastewaters					1968:1978	antibiotic-containing wastewaters	1946:1978	antibiotic-containing wastewaters	1946:1978	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	5	74	theme	pH	1316:1317	arg1	conditions					1276:1285	various operational conditions	1256:1285	various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time	1256:1356	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	2	75	theme	tetracycline	592:603	arg1	removal					581:587	the adsorptive removal	566:587	the adsorptive removal of tetracycline (TC)	566:608	Herein, the novel nanocomposite (NC) of Fe3O4-g-CN@PEI-β-CD was synthesized and employed effectively for the adsorptive removal of tetracycline (TC), the second most produced and employed antibiotic around the world.					
31848088	0	76	theme	Fe3O4-g-CN	81:90	arg1	nanocomposite					101:113	Fe3O4-g-CN@PEI-β-CD nanocomposite	81:113	Fe3O4-g-CN@PEI-β-CD nanocomposite	81:113	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	1	77	theme	environmental	311:323	arg1	matrices					325:332	all environmental matrices	307:332	all environmental matrices (e.g. water bodies, soil, etc.)	307:364	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	3	78	with	fabrication	693:703	arg1	area					755:758	a high specific surface area	731:758	a high specific surface area (57.12 m2/g)	731:771	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	3	78	with	fabrication	693:703	arg1	m2/g					767:770	57.12 m2/g	761:770	57.12 m2/g	761:770	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	5	79	theme	adsorbent	1320:1328	arg1	dose					1330:1333	adsorbent dose	1320:1333	adsorbent dose	1320:1333	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	3	80	theme	surface	747:753	arg1	area					755:758	a high specific surface area	731:758	a high specific surface area (57.12 m2/g)	731:771	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	3	80	theme	surface	747:753	arg1	m2/g					767:770	57.12 m2/g	761:770	57.12 m2/g	761:770	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	0	81	theme	PEI-β-CD	92:99	arg1	nanocomposite					101:113	Fe3O4-g-CN@PEI-β-CD nanocomposite	81:113	Fe3O4-g-CN@PEI-β-CD nanocomposite	81:113	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	8	82	theme	high	1771:1774	arg1	capacity					1787:1794	The high adsorption capacity	1767:1794	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA)	1767:1862	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	5	83	theme	initial	1290:1296	arg1	concentration					1301:1313	initial TC concentration	1290:1313	initial TC concentration	1290:1313	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	84	theme	regression	1182:1191	arg1	BRT					1199:1201	BRT	1199:1201	BRT	1199:1201	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	84	theme	regression	1182:1191	arg1	tree					1193:1196	boosted regression tree	1174:1196	boosted regression tree (BRT)	1174:1202	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	1	85	theme	water	340:344	arg1	soil					354:357	soil	354:357	soil	354:357	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	1	85	theme	water	340:344	arg1	bodies					346:351	water bodies	340:351	water bodies	340:351	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	1	85	theme	water	340:344	arg1	etc.					360:363	etc.	360:363	etc.	360:363	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	0	86	theme	regression	207:216	arg1	GRNN					234:237	GRNN	234:237	GRNN	234:237	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	0	86	theme	regression	207:216	arg1	network					225:231	general regression neural network	199:231	general regression neural network (GRNN)	199:238	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	4	87	theme	fast	879:882	arg1	rates					895:899	fast adsorption rates	879:899	fast adsorption rates towards TC and maximum adsorption capacity	879:942	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	5	88	theme	concentration	1301:1313	arg1	conditions					1276:1285	various operational conditions	1256:1285	various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time	1256:1356	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	89	theme	design	1160:1165	arg1	approaches					1128:1137	the approaches	1124:1137	the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN)	1124:1248	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	6	90	theme	all	1418:1420	arg1	predictions					1403:1413	good predictions	1398:1413	good predictions of all	1398:1420	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	5	91	theme	time	1353:1356	arg1	conditions					1276:1285	various operational conditions	1256:1285	various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time	1256:1356	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	0	92	theme	fast	24:27	arg1	adsorption					29:38	Bio-inspired, high, and fast adsorption	0:38	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media	0:73	Bio-inspired, high, and fast adsorption of tetracycline from aqueous media using Fe3O4-g-CN@PEI-β-CD nanocomposite: Modeling by response surface methodology (RSM), boosted regression tree (BRT), and general regression neural network (GRNN).					
31848088	4	93	theme	@	856:856	arg1	NC					866:867	The Fe3O4-g-CN@PEI-β-CD NC	842:867	The Fe3O4-g-CN@PEI-β-CD NC	842:867	The Fe3O4-g-CN@PEI-β-CD NC exhibited fast adsorption rates towards TC and maximum adsorption capacity on the basis of the Langmuir model reached 833.33 mg g-1, much higher than that reported by different carbon- and/or nano-based materials.					
31848088	3	94	theme	BET	828:830	arg1	analyses					832:839	BET analyses	828:839	BET analyses	828:839	The successful fabrication of the nanocomposite with a high specific surface area (57.12 m2/g) was confirmed using XRD, SEM, TEM, FTIR, TGA, EDX, and BET analyses.					
31848088	1	95	dep	matrices	325:332	arg1	soil					354:357	soil	354:357	soil	354:357	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	1	95	dep	matrices	325:332	arg1	bodies					346:351	water bodies	340:351	water bodies	340:351	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	1	95	dep	matrices	325:332	arg1	etc.					360:363	etc.	360:363	etc.	360:363	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	5	96	theme	regression	1217:1226	arg1	GRNN					1244:1247	GRNN	1244:1247	GRNN	1244:1247	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	5	96	theme	regression	1217:1226	arg1	network					1235:1241	general regression neural network	1209:1241	general regression neural network (GRNN)	1209:1248	The adsorption process was modeled using the approaches of central composite design (CCD), boosted regression tree (BRT), and general regression neural network (GRNN) under various operational conditions of initial TC concentration, pH, adsorbent dose, tempreature, and time.					
31848088	8	97	theme	optimized	1836:1844	arg1	conditions					1846:1855	the optimized conditions	1832:1855	the optimized conditions by GA	1832:1861	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
31848088	1	98	dep	bodies	346:351	arg1	e.g.					335:338	e.g.	335:338	e.g.	335:338	Because antibiotic-containing wastewaters are able to contaminate all environmental matrices (e.g. water bodies, soil, etc.), a special attention should be paid on developing appropriate materials for their remediation.					
31848088	6	99	theme	=	1525:1525	arg1	0.0014					1527:1532	MAE = 0.0014	1521:1532	MAE = 0.0014	1521:1532	The comparison of the models indicated good predictions of all, however, the BRT model was more accurate compared to the others, with R2 = 0.9992, RMSE = 0.0026, MAE = 0.0014, and AAD = 0.0028, proving that it is a powerful approach for modeling TC adsorption by Fe3O4-g-CN@PEI-β-CD nanocomposite.					
31848088	8	100	dep	in	1923:1924	arg1	situ					1926:1929	situ	1926:1929	situ	1926:1929	The high adsorption capacity along with high efficiency (98 % in the optimized conditions by GA) ensures the potential of the as-prepared nanocomposite for in situ remediation of antibiotic-containing wastewaters.					
30064747	1	0	from	fimbriata	175:183	arg1	TR					213:214	TR	213:214	TR	213:214	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	1	0	from	fimbriata	175:183	arg1	root					207:210	sweet potato tuber root	188:210	sweet potato tuber root (TR)	188:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	4	1	theme	TRs	585:587	arg1	17 days					589:595	sweet potato TRs 17 days	572:595	sweet potato TRs 17 days	572:595	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	0	2	theme	sweet	82:86	arg1	potato					88:93	sweet potato	82:93	sweet potato	82:93	Antifungal and eliciting properties of chitosan against Ceratocystis fimbriata in sweet potato.					
30064747	4	3	theme	chitosan	508:515	arg1	application					493:503	The application	489:503	The application of chitosan	489:515	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	2	4	theme	fimbriata	342:350	arg1	growth					307:312	mycelial growth	298:312	mycelial growth	298:312	The results demonstrated that chitosan effectively inhibited the mycelial growth and spore germination of C. fimbriata and directly led to the cell necrosis.					
30064747	2	4	theme	fimbriata	342:350	arg1	germination					324:334	spore germination	318:334	spore germination	318:334	The results demonstrated that chitosan effectively inhibited the mycelial growth and spore germination of C. fimbriata and directly led to the cell necrosis.					
30064747	4	5	theme	potato	578:583	arg1	17 days					589:595	sweet potato TRs 17 days	572:595	sweet potato TRs 17 days	572:595	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	4	6	theme	malondialdehyde	748:762	arg1	production					770:779	the malondialdehyde (MDA) production	744:779	the malondialdehyde (MDA) production	744:779	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	5	7	theme	eliciting	938:946	arg1	properties					948:957	its antifungal and eliciting properties	919:957	its antifungal and eliciting properties	919:957	These findings suggest that chitosan effectively controlled the infection of C. fimbriata in sweet potato TRs owing to its antifungal and eliciting properties, which induced some defense responses during storage.					
30064747	1	8	theme	black	134:138	arg1	disease					144:150	black rot disease	134:150	black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR)	134:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	5	9	theme	potato	899:904	arg1	TRs					906:908	sweet potato TRs	893:908	sweet potato TRs	893:908	These findings suggest that chitosan effectively controlled the infection of C. fimbriata in sweet potato TRs owing to its antifungal and eliciting properties, which induced some defense responses during storage.					
30064747	4	10	theme	lyase	637:641	arg1	development					557:567	the C. fimbriata development	540:567	the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL)	540:647	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	1	11	theme	Ceratocystis	162:173	arg1	fimbriata					175:183	Ceratocystis fimbriata	162:183	Ceratocystis fimbriata in sweet potato tuber root (TR)	162:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	2	12	theme	spore	318:322	arg1	germination					324:334	spore germination	318:334	spore germination	318:334	The results demonstrated that chitosan effectively inhibited the mycelial growth and spore germination of C. fimbriata and directly led to the cell necrosis.					
30064747	2	13	theme	cell	376:379	arg1	necrosis					381:388	the cell necrosis	372:388	the cell necrosis	372:388	The results demonstrated that chitosan effectively inhibited the mycelial growth and spore germination of C. fimbriata and directly led to the cell necrosis.					
30064747	3	14	theme	chitin	412:417	arg1	deposition					419:428	the chitin deposition	408:428	the chitin deposition	408:428	Chitosan altered the chitin deposition and influenced the fatty acid composition of C. fimbriata.					
30064747	4	15	theme	chitosan	718:725	arg1	treatment					727:735	the chitosan treatment	714:735	the chitosan treatment	714:735	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	1	16	theme	sweet	188:192	arg1	TR					213:214	TR	213:214	TR	213:214	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	1	16	theme	sweet	188:192	arg1	root					207:210	sweet potato tuber root	188:210	sweet potato tuber root (TR)	188:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	4	17	from	development	557:567	arg1	17 days					589:595	sweet potato TRs 17 days	572:595	sweet potato TRs 17 days	572:595	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	4	18	theme	fimbriata	547:555	arg1	development					557:567	the C. fimbriata development	540:567	the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL)	540:647	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	2	19	theme	mycelial	298:305	arg1	growth					307:312	mycelial growth	298:312	mycelial growth	298:312	The results demonstrated that chitosan effectively inhibited the mycelial growth and spore germination of C. fimbriata and directly led to the cell necrosis.					
30064747	1	20	theme	potato	194:199	arg1	TR					213:214	TR	213:214	TR	213:214	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	1	20	theme	potato	194:199	arg1	root					207:210	sweet potato tuber root	188:210	sweet potato tuber root (TR)	188:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	5	21	theme	antifungal	923:932	arg1	properties					948:957	its antifungal and eliciting properties	919:957	its antifungal and eliciting properties	919:957	These findings suggest that chitosan effectively controlled the infection of C. fimbriata in sweet potato TRs owing to its antifungal and eliciting properties, which induced some defense responses during storage.					
30064747	4	22	theme	sweet	572:576	arg1	17 days					589:595	sweet potato TRs 17 days	572:595	sweet potato TRs 17 days	572:595	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	5	23	theme	sweet	893:897	arg1	TRs					906:908	sweet potato TRs	893:908	sweet potato TRs	893:908	These findings suggest that chitosan effectively controlled the infection of C. fimbriata in sweet potato TRs owing to its antifungal and eliciting properties, which induced some defense responses during storage.					
30064747	1	24	theme	tuber	201:205	arg1	TR					213:214	TR	213:214	TR	213:214	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	1	24	theme	tuber	201:205	arg1	root					207:210	sweet potato tuber root	188:210	sweet potato tuber root (TR)	188:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	0	25	theme	chitosan	39:46	arg1	properties					25:34	properties	25:34	properties of chitosan	25:46	Antifungal and eliciting properties of chitosan against Ceratocystis fimbriata in sweet potato.					
30064747	1	26	theme	inhibitive	100:109	arg1	effects					111:117	The inhibitive effects	96:117	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR)	96:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	4	27	theme	superoxide	653:662	arg1	SOD					675:677	SOD	675:677	SOD	675:677	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	4	27	theme	superoxide	653:662	arg1	dismutase					664:672	superoxide dismutase	653:672	superoxide dismutase (SOD) activity	653:687	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	3	28	theme	fatty	449:453	arg1	composition					460:470	the fatty acid composition	445:470	the fatty acid composition of C. fimbriata	445:486	Chitosan altered the chitin deposition and influenced the fatty acid composition of C. fimbriata.					
30064747	0	29	theme	Ceratocystis	56:67	arg1	fimbriata					69:77	Ceratocystis fimbriata	56:77	Ceratocystis fimbriata in sweet potato	56:93	Antifungal and eliciting properties of chitosan against Ceratocystis fimbriata in sweet potato.					
30064747	0	30	from	fimbriata	69:77	arg1	potato					88:93	sweet potato	82:93	sweet potato	82:93	Antifungal and eliciting properties of chitosan against Ceratocystis fimbriata in sweet potato.					
30064747	3	31	theme	fimbriata	478:486	arg1	composition					460:470	the fatty acid composition	445:470	the fatty acid composition of C. fimbriata	445:486	Chitosan altered the chitin deposition and influenced the fatty acid composition of C. fimbriata.					
30064747	4	32	theme	C.	544:545	arg1	development					557:567	the C. fimbriata development	540:567	the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL)	540:647	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	1	33	theme	chitosan	122:129	arg1	effects					111:117	The inhibitive effects	96:117	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR)	96:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	4	34	theme	dismutase	664:672	arg1	activity					680:687	superoxide dismutase (SOD) activity	653:687	superoxide dismutase (SOD) activity	653:687	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	1	35	from	effects	111:117	arg1	disease					144:150	black rot disease	134:150	black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR)	134:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	4	36	theme	25 °C.	608:613	arg1	PAL					644:646	PAL	644:646	PAL	644:646	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	4	36	theme	25 °C.	608:613	arg1	lyase					637:641	storage 25 °C. Phenylalanine ammonia lyase	600:641	storage 25 °C. Phenylalanine ammonia lyase (PAL)	600:647	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	5	37	theme	defense	979:985	arg1	responses					987:995	some defense responses	974:995	some defense responses during storage	974:1010	These findings suggest that chitosan effectively controlled the infection of C. fimbriata in sweet potato TRs owing to its antifungal and eliciting properties, which induced some defense responses during storage.					
30064747	5	38	from	infection	864:872	arg1	TRs					906:908	sweet potato TRs	893:908	sweet potato TRs	893:908	These findings suggest that chitosan effectively controlled the infection of C. fimbriata in sweet potato TRs owing to its antifungal and eliciting properties, which induced some defense responses during storage.					
30064747	3	39	theme	C.	475:476	arg1	fimbriata					478:486	C. fimbriata	475:486	C. fimbriata	475:486	Chitosan altered the chitin deposition and influenced the fatty acid composition of C. fimbriata.					
30064747	4	40	theme	storage	600:606	arg1	PAL					644:646	PAL	644:646	PAL	644:646	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	4	40	theme	storage	600:606	arg1	lyase					637:641	storage 25 °C. Phenylalanine ammonia lyase	600:641	storage 25 °C. Phenylalanine ammonia lyase (PAL)	600:647	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	3	41	theme	acid	455:458	arg1	composition					460:470	the fatty acid composition	445:470	the fatty acid composition of C. fimbriata	445:486	Chitosan altered the chitin deposition and influenced the fatty acid composition of C. fimbriata.					
30064747	1	42	theme	rot	140:142	arg1	disease					144:150	black rot disease	134:150	black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR)	134:215	The inhibitive effects of chitosan on black rot disease caused by Ceratocystis fimbriata in sweet potato tuber root (TR) were evaluated.					
30064747	4	43	theme	ammonia	629:635	arg1	PAL					644:646	PAL	644:646	PAL	644:646	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	4	43	theme	ammonia	629:635	arg1	lyase					637:641	storage 25 °C. Phenylalanine ammonia lyase	600:641	storage 25 °C. Phenylalanine ammonia lyase (PAL)	600:647	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	5	44	theme	fimbriata	880:888	arg1	infection					864:872	the infection	860:872	the infection of C. fimbriata in sweet potato TRs	860:908	These findings suggest that chitosan effectively controlled the infection of C. fimbriata in sweet potato TRs owing to its antifungal and eliciting properties, which induced some defense responses during storage.					
30064747	4	45	theme	Phenylalanine	615:627	arg1	PAL					644:646	PAL	644:646	PAL	644:646	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
30064747	4	45	theme	Phenylalanine	615:627	arg1	lyase					637:641	storage 25 °C. Phenylalanine ammonia lyase	600:641	storage 25 °C. Phenylalanine ammonia lyase (PAL)	600:647	The application of chitosan effectively controlled the C. fimbriata development in sweet potato TRs 17 days of storage 25 °C. Phenylalanine ammonia lyase (PAL) and superoxide dismutase (SOD) activity were clearly enhanced by the chitosan treatment, while the malondialdehyde (MDA) production was not increased.					
31020858	8	0	with	jelly	1292:1296	arg1	results					1331:1337	acceptable results	1320:1337	acceptable results	1320:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	8	1	from	analyte	1263:1269	arg1	jelly					1292:1296	jelly	1292:1296	jelly	1292:1296	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	8	1	from	analyte	1263:1269	arg1	candy					1285:1289	commercial candy	1274:1289	commercial candy	1274:1289	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	8	1	from	analyte	1263:1269	arg1	drinks					1308:1313	soft drinks	1303:1313	soft drinks with acceptable results	1303:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	1	2	theme	chitosan/graphene	171:187	arg1	CHIT/GO					196:202	CHIT/GO	196:202	CHIT/GO	196:202	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	2	theme	chitosan/graphene	171:187	arg1	oxide					189:193	chitosan/graphene oxide	171:193	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	171:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	8	3	theme	acceptable	1320:1329	arg1	results					1331:1337	acceptable results	1320:1337	acceptable results	1320:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	0	4	theme	modified	107:114	arg1	electrode					130:138	CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode	71:138	CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode	71:138	An electrochemical sensor for the determination of tartrazine based on CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode.					
31020858	3	5	theme	synergistic	736:746	arg1	influence					748:756	synergistic influence	736:756	synergistic influence	736:756	The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals.					
31020858	2	6	theme	MWCNTs	596:601	arg1	properties					520:529	the signal amplification properties	495:529	the signal amplification properties of GO	495:535	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	2	6	theme	MWCNTs	596:601	arg1	properties					582:591	the excellent electronic and antifouling properties	541:591	the excellent electronic and antifouling properties of MWCNTs	541:601	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	5	7	dep	100 mg	930:935	arg1	to					927:928	to	927:928	to	927:928	The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1.					
31020858	5	8	theme	0.018	979:983	arg1	μM-1					988:991	0.018 μA μM-1	979:991	0.018 μA μM-1	979:991	The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1.					
31020858	1	9	from	dyes	399:402	arg1	products					412:419	food products	407:419	food products	407:419	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	4	10	theme	optimum	803:809	arg1	conditions					811:820	The optimum conditions	799:820	The optimum conditions	799:820	The optimum conditions were found at pH 7, 30 s, and 0.3 Vs-1.					
31020858	8	11	theme	TZ	1260:1261	arg1	analyte					1263:1269	TZ analyte	1260:1269	TZ analyte in commercial candy, jelly, and soft drinks with acceptable results	1260:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	0	12	theme	carbon	123:128	arg1	electrode					130:138	CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode	71:138	CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode	71:138	An electrochemical sensor for the determination of tartrazine based on CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode.					
31020858	1	13	theme	Tartrazine	372:381	arg1	determination					355:367	the determination	351:367	the determination of Tartrazine (TZ), synthetic dyes in food products	351:419	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	8	14	from	determination	1243:1255	arg1	jelly					1292:1296	jelly	1292:1296	jelly	1292:1296	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	8	14	from	determination	1243:1255	arg1	candy					1285:1289	commercial candy	1274:1289	commercial candy	1274:1289	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	8	14	from	determination	1243:1255	arg1	drinks					1308:1313	soft drinks	1303:1313	soft drinks with acceptable results	1303:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	8	15	theme	soft	1303:1306	arg1	drinks					1308:1313	soft drinks	1303:1313	soft drinks with acceptable results	1303:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	1	16	theme	oxide	189:193	arg1	AuNPs					264:268	AuNPs	264:268	AuNPs	264:268	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	16	theme	oxide	189:193	arg1	nanoparticles					249:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles	171:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	171:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	0	17	theme	glassy	116:121	arg1	electrode					130:138	CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode	71:138	CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode	71:138	An electrochemical sensor for the determination of tartrazine based on CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode.					
31020858	5	18	with	mL-1	937:940	arg1	sensitivity					964:974	a sensitivity	962:974	a sensitivity of 0.018 μA μM-1	962:991	The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1.					
31020858	2	19	theme	amplification	506:518	arg1	properties					520:529	the signal amplification properties	495:529	the signal amplification properties of GO	495:535	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	0	20	theme	electrochemical	3:17	arg1	sensor					19:24	An electrochemical sensor	0:24	An electrochemical sensor for the determination of tartrazine	0:60	An electrochemical sensor for the determination of tartrazine based on CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode.					
31020858	3	21	theme	signals	790:796	arg1	increment					765:773	the increment	761:773	the increment of the current signals	761:796	The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals.					
31020858	8	22	from	candy	1285:1289	arg1	determination					1243:1255	the determination	1239:1255	the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results	1239:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	6	23	theme	quantification	1027:1040	arg1	1.45					1056:1059	1.45	1056:1059	1.45	1056:1059	The limit of detection (LOD) and quantification obtained were 1.45 and 4.83 mg mL-1, respectively.					
31020858	6	23	theme	quantification	1027:1040	arg1	limit					998:1002	The limit	994:1002	The limit of detection (LOD) and quantification obtained	994:1049	The limit of detection (LOD) and quantification obtained were 1.45 and 4.83 mg mL-1, respectively.					
31020858	7	24	from	%	1192:1192	arg1	determination					1097:1109	The determination	1093:1109	The determination of TZ in spiked samples	1093:1133	The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%.					
31020858	7	24	from	%	1192:1192	arg1	reliable					1139:1146	reliable	1139:1146	reliable	1139:1146	The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%.					
31020858	8	25	theme	developed	1199:1207	arg1	sensor					1209:1214	The developed sensor	1195:1214	The developed sensor	1195:1214	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	2	26	theme	electrochemical	426:440	arg1	sensors					442:448	The electrochemical sensors	422:448	The electrochemical sensors	422:448	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	2	27	theme	excellent	545:553	arg1	properties					582:591	the excellent electronic and antifouling properties	541:591	the excellent electronic and antifouling properties of MWCNTs	541:601	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	1	28	theme	/multi-walled	204:216	arg1	AuNPs					264:268	AuNPs	264:268	AuNPs	264:268	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	28	theme	/multi-walled	204:216	arg1	nanoparticles					249:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles	171:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	171:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	3	29	theme	current	782:788	arg1	signals					790:796	the current signals	778:796	the current signals	778:796	The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals.					
31020858	7	30	with	reliable	1139:1146	arg1	percentage					1162:1171	recovery percentage	1153:1171	recovery percentage	1153:1171	The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%.					
31020858	1	31	theme	carbon	218:223	arg1	AuNPs					264:268	AuNPs	264:268	AuNPs	264:268	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	31	theme	carbon	218:223	arg1	nanoparticles					249:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles	171:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	171:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	7	32	theme	TZ	1114:1115	arg1	determination					1097:1109	The determination	1093:1109	The determination of TZ in spiked samples	1093:1133	The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%.					
31020858	7	32	theme	TZ	1114:1115	arg1	reliable					1139:1146	reliable	1139:1146	reliable	1139:1146	The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%.					
31020858	1	33	theme	glassy	296:301	arg1	electrode					310:318	glassy carbon electrode	296:318	glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE)	296:345	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	33	theme	glassy	296:301	arg1	CHIT/GO/MWCNTs/AuNPs/GCE					321:344	CHIT/GO/MWCNTs/AuNPs/GCE	321:344	CHIT/GO/MWCNTs/AuNPs/GCE	321:344	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	7	34	dep	109.0	1187:1191	arg1	to					1184:1185	to	1184:1185	to	1184:1185	The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%.					
31020858	7	35	theme	recovery	1153:1160	arg1	percentage					1162:1171	recovery percentage	1153:1171	recovery percentage	1153:1171	The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%.					
31020858	8	36	theme	commercial	1274:1283	arg1	candy					1285:1289	commercial candy	1274:1289	commercial candy	1274:1289	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	8	37	from	jelly	1292:1296	arg1	determination					1243:1255	the determination	1239:1255	the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results	1239:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	1	38	theme	nanotubes	225:233	arg1	AuNPs					264:268	AuNPs	264:268	AuNPs	264:268	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	38	theme	nanotubes	225:233	arg1	nanoparticles					249:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles	171:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	171:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	39	theme	carbon	303:308	arg1	electrode					310:318	glassy carbon electrode	296:318	glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE)	296:345	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	39	theme	carbon	303:308	arg1	CHIT/GO/MWCNTs/AuNPs/GCE					321:344	CHIT/GO/MWCNTs/AuNPs/GCE	321:344	CHIT/GO/MWCNTs/AuNPs/GCE	321:344	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	2	40	theme	GO	534:535	arg1	properties					520:529	the signal amplification properties	495:529	the signal amplification properties of GO	495:535	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	2	40	theme	GO	534:535	arg1	properties					582:591	the excellent electronic and antifouling properties	541:591	the excellent electronic and antifouling properties of MWCNTs	541:601	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	1	41	theme	synthetic	389:397	arg1	Tartrazine					372:381	Tartrazine	372:381	Tartrazine (TZ)	372:386	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	41	theme	synthetic	389:397	arg1	dyes					399:402	synthetic dyes	389:402	synthetic dyes in food products	389:419	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	7	42	theme	spiked	1120:1125	arg1	samples					1127:1133	spiked samples	1120:1133	spiked samples	1120:1133	The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%.					
31020858	5	43	theme	modified	866:873	arg1	GCE					875:877	The modified GCE	862:877	The modified GCE	862:877	The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1.					
31020858	2	44	theme	signal	499:504	arg1	properties					520:529	the signal amplification properties	495:529	the signal amplification properties of GO	495:535	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	0	45	theme	tartrazine	51:60	arg1	determination					34:46	the determination	30:46	the determination of tartrazine	30:60	An electrochemical sensor for the determination of tartrazine based on CHIT/GO/MWCNTs/AuNPs composite film modified glassy carbon electrode.					
31020858	5	46	theme	linear	895:900	arg1	response					902:909	a linear response	893:909	a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1	893:991	The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1.					
31020858	6	47	theme	detection	1007:1015	arg1	1.45					1056:1059	1.45	1056:1059	1.45	1056:1059	The limit of detection (LOD) and quantification obtained were 1.45 and 4.83 mg mL-1, respectively.					
31020858	6	47	theme	detection	1007:1015	arg1	limit					998:1002	The limit	994:1002	The limit of detection (LOD) and quantification obtained	994:1049	The limit of detection (LOD) and quantification obtained were 1.45 and 4.83 mg mL-1, respectively.					
31020858	3	48	theme	transfer	664:671	arg1	materials					673:681	as superior electron transfer materials	643:681	as superior electron transfer materials	643:681	The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals.					
31020858	8	49	from	drinks	1308:1313	arg1	determination					1243:1255	the determination	1239:1255	the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results	1239:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	2	50	theme	antifouling	570:580	arg1	properties					582:591	the excellent electronic and antifouling properties	541:591	the excellent electronic and antifouling properties of MWCNTs	541:601	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	8	51	with	candy	1285:1289	arg1	results					1331:1337	acceptable results	1320:1337	acceptable results	1320:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	3	52	theme	electron	655:662	arg1	transfer					664:671	electron transfer	655:671	as superior electron transfer materials	643:681	The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals.					
31020858	1	53	theme	food	407:410	arg1	products					412:419	food products	407:419	food products	407:419	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	5	54	theme	100 mg	930:935	arg1	0.99037					948:954	0.99037	948:954	0.99037	948:954	The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1.					
31020858	5	54	theme	100 mg	930:935	arg1	mL-1					937:940	10 to 100 mg mL-1	924:940	10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1	924:991	The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1.					
31020858	1	55	theme	MWCNTs	236:241	arg1	AuNPs					264:268	AuNPs	264:268	AuNPs	264:268	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	55	theme	MWCNTs	236:241	arg1	nanoparticles					249:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles	171:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	171:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	2	56	theme	electronic	555:564	arg1	properties					582:591	the excellent electronic and antifouling properties	541:591	the excellent electronic and antifouling properties of MWCNTs	541:601	The electrochemical sensors found it to be highly sensitive by combining the signal amplification properties of GO and the excellent electronic and antifouling properties of MWCNTs.					
31020858	8	57	with	drinks	1308:1313	arg1	results					1331:1337	acceptable results	1320:1337	acceptable results	1320:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	3	58	theme	superior	646:653	arg1	materials					673:681	as superior electron transfer materials	643:681	as superior electron transfer materials	643:681	The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals.					
31020858	4	59	located	found	827:831	arg2	conditions					811:820	The optimum conditions	799:820	The optimum conditions	799:820	The optimum conditions were found at pH 7, 30 s, and 0.3 Vs-1.					
31020858	4	59	located	found	827:831	arg1	Vs-1					856:859	0.3 Vs-1	852:859	0.3 Vs-1	852:859	The optimum conditions were found at pH 7, 30 s, and 0.3 Vs-1.					
31020858	4	59	located	found	827:831	arg1	pH					836:837	pH 7	836:839	pH 7	836:839	The optimum conditions were found at pH 7, 30 s, and 0.3 Vs-1.					
31020858	4	59	located	found	827:831	arg1	30 s					842:845	30 s	842:845	30 s	842:845	The optimum conditions were found at pH 7, 30 s, and 0.3 Vs-1.					
31020858	1	60	theme	novel	143:147	arg1	film					163:166	A novel nanocomposite film	141:166	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	141:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	8	61	theme	analyte	1263:1269	arg1	determination					1243:1255	the determination	1239:1255	the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results	1239:1337	The developed sensor successfully tested in the determination of TZ analyte in commercial candy, jelly, and soft drinks with acceptable results.					
31020858	3	62	theme	intercalation	697:709	arg1	properties					711:720	intercalation properties	697:720	intercalation properties which provide synergistic influence on the increment of the current signals	697:796	The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals.					
31020858	5	63	theme	μA	985:986	arg1	μM-1					988:991	0.018 μA μM-1	979:991	0.018 μA μM-1	979:991	The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1.					
31020858	1	64	theme	nanocomposite	149:161	arg1	film					163:166	A novel nanocomposite film	141:166	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	141:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	3	65	contain	possesses	687:695	arg2	properties					711:720	intercalation properties	697:720	intercalation properties which provide synergistic influence on the increment of the current signals	697:796	The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals.					
31020858	3	65	contain	possesses	687:695	arg1	CHIT/GO/MWCNTs/AuNPs/GCE					608:631	The CHIT/GO/MWCNTs/AuNPs/GCE	604:631	The CHIT/GO/MWCNTs/AuNPs/GCE	604:631	The CHIT/GO/MWCNTs/AuNPs/GCE exhibited as superior electron transfer materials and possesses intercalation properties which provide synergistic influence on the increment of the current signals.					
31020858	1	66	theme	/gold	243:247	arg1	AuNPs					264:268	AuNPs	264:268	AuNPs	264:268	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	1	66	theme	/gold	243:247	arg1	nanoparticles					249:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles	171:261	chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	171:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
31020858	7	67	from	determination	1097:1109	arg1	samples					1127:1133	spiked samples	1120:1133	spiked samples	1120:1133	The determination of TZ in spiked samples was reliable with recovery percentage from 94.52 to 109.0%.					
31020858	5	68	theme	μM-1	988:991	arg1	sensitivity					964:974	a sensitivity	962:974	a sensitivity of 0.018 μA μM-1	962:991	The modified GCE obtained with a linear response ranging from 10 to 100 mg mL-1 (r2 = 0.99037) with a sensitivity of 0.018 μA μM-1.					
31020858	6	69	theme	4.83 mg	1065:1071	arg1	mL-1					1073:1076	4.83 mg mL-1	1065:1076	4.83 mg mL-1	1065:1076	The limit of detection (LOD) and quantification obtained were 1.45 and 4.83 mg mL-1, respectively.					
31020858	1	70	theme	nanoparticles	249:261	arg1	film					163:166	A novel nanocomposite film	141:166	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs)	141:269	A novel nanocomposite film of chitosan/graphene oxide (CHIT/GO)/multi-walled carbon nanotubes (MWCNTs)/gold nanoparticles (AuNPs) was applied to fabricate glassy carbon electrode (CHIT/GO/MWCNTs/AuNPs/GCE) for the determination of Tartrazine (TZ), synthetic dyes in food products.					
29693223	3	0	theme	87-year-old	342:352	arg1	man					354:356	An 87-year-old man	339:356	An 87-year-old man	339:356	An 87-year-old man presented with acute shock.					
29693223	5	1	theme	Surgicel®	590:598	arg1	agents					620:625	Hydrofit® and Surgicel® surgical hemostatic agents	576:625	Hydrofit® and Surgicel® surgical hemostatic agents	576:625	We describe a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents.					
29693223	2	2	theme	left	276:279	arg1	repair					293:298	left ventricular repair	276:298	left ventricular repair	276:298	In these cases, cardiopulmonary bypass (CPB) should be performed for left ventricular repair, but can impact hemodynamic stability.					
29693223	2	3	theme	ventricular	281:291	arg1	repair					293:298	left ventricular repair	276:298	left ventricular repair	276:298	In these cases, cardiopulmonary bypass (CPB) should be performed for left ventricular repair, but can impact hemodynamic stability.					
29693223	5	4	theme	surgical	600:607	arg1	agents					620:625	Hydrofit® and Surgicel® surgical hemostatic agents	576:625	Hydrofit® and Surgicel® surgical hemostatic agents	576:625	We describe a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents.					
29693223	6	5	from	composite	694:702	arg1	site					720:723	the bleeding site	707:723	the bleeding site	707:723	We simply placed and manually pressed the Hydrofit® and Surgicel® composite on the bleeding site.					
29693223	6	6	theme	bleeding	711:718	arg1	site					720:723	the bleeding site	707:723	the bleeding site	707:723	We simply placed and manually pressed the Hydrofit® and Surgicel® composite on the bleeding site.					
29693223	0	7	with	infarction	66:75	arg1	Hydrofit®					82:90	Hydrofit®	82:90	Hydrofit®	82:90	Off-pump hemostasis for left ventricular rupture after myocardial infarction with Hydrofit® and Surgicel®.					
29693223	0	7	with	infarction	66:75	arg1	Surgicel®					96:104	Surgicel®	96:104	Surgicel®	96:104	Off-pump hemostasis for left ventricular rupture after myocardial infarction with Hydrofit® and Surgicel®.					
29693223	1	8	theme	catastrophic	155:166	arg1	complication					168:179	a catastrophic complication	153:179	a catastrophic complication of myocardial infarction	153:204	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	8	theme	catastrophic	155:166	arg1	wall					129:132	Left ventricular free wall rupture	107:140	Left ventricular free wall rupture (LVFWR)	107:148	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	5	9	theme	emergency	550:558	arg1	operation					560:568	emergency operation	550:568	emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents	550:625	We describe a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents.					
29693223	5	10	theme	reproducible	480:491	arg1	technique					493:501	a simple, effective, and reproducible technique	455:501	a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents	455:625	We describe a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents.					
29693223	3	11	theme	acute	373:377	arg1	shock					379:383	acute shock	373:383	acute shock	373:383	An 87-year-old man presented with acute shock.					
29693223	0	12	theme	Off-pump	0:7	arg1	hemostasis					9:18	Off-pump hemostasis	0:18	Off-pump hemostasis for left ventricular rupture after myocardial infarction with Hydrofit® and Surgicel®.	0:105	Off-pump hemostasis for left ventricular rupture after myocardial infarction with Hydrofit® and Surgicel®.					
29693223	5	13	theme	hemostatic	609:618	arg1	agents					620:625	Hydrofit® and Surgicel® surgical hemostatic agents	576:625	Hydrofit® and Surgicel® surgical hemostatic agents	576:625	We describe a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents.					
29693223	6	14	theme	Surgicel®	684:692	arg1	composite					694:702	the Hydrofit® and Surgicel® composite	666:702	the Hydrofit® and Surgicel® composite on the bleeding site	666:723	We simply placed and manually pressed the Hydrofit® and Surgicel® composite on the bleeding site.					
29693223	1	15	theme	myocardial	184:193	arg1	infarction					195:204	myocardial infarction	184:204	myocardial infarction	184:204	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	0	16	theme	left	24:27	arg1	rupture					41:47	left ventricular rupture	24:47	left ventricular rupture	24:47	Off-pump hemostasis for left ventricular rupture after myocardial infarction with Hydrofit® and Surgicel®.					
29693223	5	17	theme	Hydrofit®	576:584	arg1	agents					620:625	Hydrofit® and Surgicel® surgical hemostatic agents	576:625	Hydrofit® and Surgicel® surgical hemostatic agents	576:625	We describe a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents.					
29693223	1	18	theme	Left	107:110	arg1	wall					129:132	Left ventricular free wall rupture	107:140	Left ventricular free wall rupture (LVFWR)	107:148	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	18	theme	Left	107:110	arg1	LVFWR					143:147	LVFWR	143:147	LVFWR	143:147	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	18	theme	Left	107:110	arg1	complication					168:179	a catastrophic complication	153:179	a catastrophic complication of myocardial infarction	153:204	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	5	19	theme	simple	457:462	arg1	technique					493:501	a simple, effective, and reproducible technique	455:501	a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents	455:625	We describe a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents.					
29693223	1	20	theme	infarction	195:204	arg1	complication					168:179	a catastrophic complication	153:179	a catastrophic complication of myocardial infarction	153:204	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	20	theme	infarction	195:204	arg1	wall					129:132	Left ventricular free wall rupture	107:140	Left ventricular free wall rupture (LVFWR)	107:148	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	21	theme	ventricular	112:122	arg1	wall					129:132	Left ventricular free wall rupture	107:140	Left ventricular free wall rupture (LVFWR)	107:148	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	21	theme	ventricular	112:122	arg1	LVFWR					143:147	LVFWR	143:147	LVFWR	143:147	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	21	theme	ventricular	112:122	arg1	complication					168:179	a catastrophic complication	153:179	a catastrophic complication of myocardial infarction	153:204	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	6	22	theme	Hydrofit®	670:678	arg1	composite					694:702	the Hydrofit® and Surgicel® composite	666:702	the Hydrofit® and Surgicel® composite on the bleeding site	666:723	We simply placed and manually pressed the Hydrofit® and Surgicel® composite on the bleeding site.					
29693223	5	23	theme	effective	465:473	arg1	technique					493:501	a simple, effective, and reproducible technique	455:501	a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents	455:625	We describe a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents.					
29693223	0	24	theme	ventricular	29:39	arg1	rupture					41:47	left ventricular rupture	24:47	left ventricular rupture	24:47	Off-pump hemostasis for left ventricular rupture after myocardial infarction with Hydrofit® and Surgicel®.					
29693223	2	25	dep	cardiopulmonary	223:237	arg1	bypass					239:244	bypass	239:244	bypass	239:244	In these cases, cardiopulmonary bypass (CPB) should be performed for left ventricular repair, but can impact hemodynamic stability.					
29693223	0	26	theme	myocardial	55:64	arg1	infarction					66:75	myocardial infarction	55:75	myocardial infarction with Hydrofit® and Surgicel®	55:104	Off-pump hemostasis for left ventricular rupture after myocardial infarction with Hydrofit® and Surgicel®.					
29693223	5	27	theme	LVFWR	532:536	arg1	site					538:541	the LVFWR site	528:541	the LVFWR site	528:541	We describe a simple, effective, and reproducible technique to achieve hemostasis at the LVFWR site during emergency operation using Hydrofit® and Surgicel® surgical hemostatic agents.					
29693223	1	28	theme	free	124:127	arg1	wall					129:132	Left ventricular free wall rupture	107:140	Left ventricular free wall rupture (LVFWR)	107:148	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	28	theme	free	124:127	arg1	LVFWR					143:147	LVFWR	143:147	LVFWR	143:147	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	28	theme	free	124:127	arg1	complication					168:179	a catastrophic complication	153:179	a catastrophic complication of myocardial infarction	153:204	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	7	29	theme	CPB	778:780	arg1	establishment					782:794	CPB establishment	778:794	CPB establishment	778:794	This technique provides complete hemostasis without CPB establishment.					
29693223	2	30	theme	hemodynamic	316:326	arg1	stability					328:336	hemodynamic stability	316:336	hemodynamic stability	316:336	In these cases, cardiopulmonary bypass (CPB) should be performed for left ventricular repair, but can impact hemodynamic stability.					
29693223	1	31	theme	rupture	134:140	arg1	wall					129:132	Left ventricular free wall rupture	107:140	Left ventricular free wall rupture (LVFWR)	107:148	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	31	theme	rupture	134:140	arg1	LVFWR					143:147	LVFWR	143:147	LVFWR	143:147	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	1	31	theme	rupture	134:140	arg1	complication					168:179	a catastrophic complication	153:179	a catastrophic complication of myocardial infarction	153:204	Left ventricular free wall rupture (LVFWR) is a catastrophic complication of myocardial infarction.					
29693223	4	32	theme	myocardial	420:429	arg1	infarction					431:440	myocardial infarction	420:440	myocardial infarction	420:440	He was diagnosed with LVFWR after myocardial infarction.					
29693223	7	33	theme	complete	750:757	arg1	hemostasis					759:768	complete hemostasis	750:768	complete hemostasis	750:768	This technique provides complete hemostasis without CPB establishment.					
31887952	4	0	dep	showed	772:777	arg1	compared					825:832	compared	825:832	showed cell viability improvement between 7% and 17% compared to the control sample	772:854	Biocompatibility tests performed on human osteoblast cells, showed cell viability improvement between 7% and 17% compared to the control sample.					
31887952	2	1	theme	Diffusion	394:402	arg1	System					419:424	an Automated Transdermal Diffusion Cells Sampling System	369:424	an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method	369:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	1	2	theme	anti-inflammatory	257:273	arg1	drug					275:278	the non-steroid anti-inflammatory drug	241:278	the non-steroid anti-inflammatory drug	241:278	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	2	3	theme	Transdermal	382:392	arg1	System					419:424	an Automated Transdermal Diffusion Cells Sampling System	369:424	an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method	369:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	2	4	with	combination	429:439	arg1	spectroscopy					453:464	UV-VIS spectroscopy	446:464	UV-VIS spectroscopy as the released drug concentration determination method	446:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	3	5	theme	first	583:587	arg1	hours					593:597	the first six hours	579:597	the first six hours	579:597	The results showed that most of the drug is released in the first six hours, whereas the overall released amount could be tailored through changes in the multi-layered coating composition.					
31887952	5	6	theme	prepared	1016:1023	arg1	osteointegration					1036:1051	the prepared materials' osteointegration	1012:1051	the prepared materials' osteointegration boosting capacity	1012:1069	The expression of proteins playing important roles in extracellular matrix production and functioning was performed in order to obtain additional proof of the prepared materials' osteointegration boosting capacity.					
31887952	4	7	theme	osteoblast	754:763	arg1	cells					765:769	human osteoblast cells	748:769	human osteoblast cells	748:769	Biocompatibility tests performed on human osteoblast cells, showed cell viability improvement between 7% and 17% compared to the control sample.					
31887952	6	8	theme	316LVM	1192:1197	arg1	steel					1209:1213	AISI 316LVM stainless steel	1187:1213	AISI 316LVM stainless steel	1187:1213	Finally, electrochemical measurements confirmed that the coatings do not influence the corrosion susceptibility of AISI 316LVM stainless steel.					
31887952	4	9	theme	human	748:752	arg1	cells					765:769	human osteoblast cells	748:769	human osteoblast cells	748:769	Biocompatibility tests performed on human osteoblast cells, showed cell viability improvement between 7% and 17% compared to the control sample.					
31887952	5	10	theme	proteins	875:882	arg1	expression					861:870	The expression	857:870	The expression of proteins playing important roles in extracellular matrix production and functioning	857:957	The expression of proteins playing important roles in extracellular matrix production and functioning was performed in order to obtain additional proof of the prepared materials' osteointegration boosting capacity.					
31887952	1	11	theme	systematic	175:184	arg1	testing					186:192	systematic testing	175:192	systematic testing	175:192	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	0	12	theme	osteogenic	112:121	arg1	potential					123:131	osteogenic potential	112:131	osteogenic potential	112:131	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI 316LVM for controlled drug delivery, and improved osteogenic potential.					
31887952	0	13	theme	Carboxymethyl	0:12	arg1	coatings					45:52	Carboxymethyl cellulose/diclofenac bioactive coatings	0:52	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI	0:60	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI 316LVM for controlled drug delivery, and improved osteogenic potential.					
31887952	6	14	theme	corrosion	1159:1167	arg1	susceptibility					1169:1182	the corrosion susceptibility	1155:1182	the corrosion susceptibility of AISI 316LVM stainless steel	1155:1213	Finally, electrochemical measurements confirmed that the coatings do not influence the corrosion susceptibility of AISI 316LVM stainless steel.					
31887952	2	15	theme	determination	501:513	arg1	method					515:520	the released drug concentration determination method	469:520	the released drug concentration determination method	469:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	1	16	dep	preparation	159:169	arg1	the					155:157	the	155:157	the	155:157	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	3	17	from	changes	662:668	arg1	composition					699:709	the multi-layered coating composition	673:709	the multi-layered coating composition	673:709	The results showed that most of the drug is released in the first six hours, whereas the overall released amount could be tailored through changes in the multi-layered coating composition.					
31887952	5	18	theme	extracellular	911:923	arg1	production					932:941	extracellular matrix production	911:941	extracellular matrix production	911:941	The expression of proteins playing important roles in extracellular matrix production and functioning was performed in order to obtain additional proof of the prepared materials' osteointegration boosting capacity.					
31887952	2	19	theme	concentration	487:499	arg1	method					515:520	the released drug concentration determination method	469:520	the released drug concentration determination method	469:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	4	20	theme	viability	784:792	arg1	improvement					794:804	cell viability improvement	779:804	cell viability improvement between 7% and 17%	779:823	Biocompatibility tests performed on human osteoblast cells, showed cell viability improvement between 7% and 17% compared to the control sample.					
31887952	4	21	theme	Biocompatibility	712:727	arg1	tests					729:733	Biocompatibility tests	712:733	Biocompatibility tests	712:733	Biocompatibility tests performed on human osteoblast cells, showed cell viability improvement between 7% and 17% compared to the control sample.					
31887952	2	22	theme	drug	482:485	arg1	method					515:520	the released drug concentration determination method	469:520	the released drug concentration determination method	469:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	1	23	theme	carboxymethyl	306:318	arg1	cellulose					320:328	carboxymethyl cellulose	306:328	carboxymethyl cellulose	306:328	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	2	24	theme	release	336:342	arg1	testing					344:350	Drug release testing	331:350	Drug release testing	331:350	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	1	25	theme	novel	199:203	arg1	coating					219:225	a novel multi-layered coating	197:225	a novel multi-layered coating	197:225	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	0	26	theme	bioactive	35:43	arg1	coatings					45:52	Carboxymethyl cellulose/diclofenac bioactive coatings	0:52	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI	0:60	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI 316LVM for controlled drug delivery, and improved osteogenic potential.					
31887952	5	27	theme	additional	992:1001	arg1	proof					1003:1007	additional proof	992:1007	additional proof of the prepared materials' osteointegration boosting capacity	992:1069	The expression of proteins playing important roles in extracellular matrix production and functioning was performed in order to obtain additional proof of the prepared materials' osteointegration boosting capacity.					
31887952	3	28	theme	multi-layered	677:689	arg1	composition					699:709	the multi-layered coating composition	673:709	the multi-layered coating composition	673:709	The results showed that most of the drug is released in the first six hours, whereas the overall released amount could be tailored through changes in the multi-layered coating composition.					
31887952	2	29	theme	Drug	331:334	arg1	testing					344:350	Drug release testing	331:350	Drug release testing	331:350	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	1	30	theme	multi-layered	205:217	arg1	coating					219:225	a novel multi-layered coating	197:225	a novel multi-layered coating	197:225	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	6	31	theme	AISI	1187:1190	arg1	steel					1209:1213	AISI 316LVM stainless steel	1187:1213	AISI 316LVM stainless steel	1187:1213	Finally, electrochemical measurements confirmed that the coatings do not influence the corrosion susceptibility of AISI 316LVM stainless steel.					
31887952	3	32	theme	coating	691:697	arg1	composition					699:709	the multi-layered coating composition	673:709	the multi-layered coating composition	673:709	The results showed that most of the drug is released in the first six hours, whereas the overall released amount could be tailored through changes in the multi-layered coating composition.					
31887952	5	33	theme	matrix	925:930	arg1	production					932:941	extracellular matrix production	911:941	extracellular matrix production	911:941	The expression of proteins playing important roles in extracellular matrix production and functioning was performed in order to obtain additional proof of the prepared materials' osteointegration boosting capacity.					
31887952	1	34	theme	coating	219:225	arg1	preparation					159:169	preparation	159:169	preparation	159:169	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	1	34	theme	coating	219:225	arg1	testing					186:192	systematic testing	175:192	systematic testing	175:192	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	5	35	theme	important	892:900	arg1	roles					902:906	important roles	892:906	important roles	892:906	The expression of proteins playing important roles in extracellular matrix production and functioning was performed in order to obtain additional proof of the prepared materials' osteointegration boosting capacity.					
31887952	2	36	theme	UV-VIS	446:451	arg1	spectroscopy					453:464	UV-VIS spectroscopy	446:464	UV-VIS spectroscopy as the released drug concentration determination method	446:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	3	37	theme	overall	612:618	arg1	amount					629:634	the overall released amount	608:634	the overall released amount	608:634	The results showed that most of the drug is released in the first six hours, whereas the overall released amount could be tailored through changes in the multi-layered coating composition.					
31887952	2	38	from	System	419:424	arg1	combination					429:439	combination	429:439	combination with UV-VIS spectroscopy as the released drug concentration determination method	429:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	2	39	theme	Automated	372:380	arg1	System					419:424	an Automated Transdermal Diffusion Cells Sampling System	369:424	an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method	369:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	3	40	theme	released	620:627	arg1	amount					629:634	the overall released amount	608:634	the overall released amount	608:634	The results showed that most of the drug is released in the first six hours, whereas the overall released amount could be tailored through changes in the multi-layered coating composition.					
31887952	6	41	theme	steel	1209:1213	arg1	susceptibility					1169:1182	the corrosion susceptibility	1155:1182	the corrosion susceptibility of AISI 316LVM stainless steel	1155:1213	Finally, electrochemical measurements confirmed that the coatings do not influence the corrosion susceptibility of AISI 316LVM stainless steel.					
31887952	5	42	theme	osteointegration	1036:1051	arg1	proof					1003:1007	additional proof	992:1007	additional proof of the prepared materials' osteointegration boosting capacity	992:1069	The expression of proteins playing important roles in extracellular matrix production and functioning was performed in order to obtain additional proof of the prepared materials' osteointegration boosting capacity.					
31887952	6	43	theme	electrochemical	1081:1095	arg1	measurements					1097:1108	electrochemical measurements	1081:1108	electrochemical measurements	1081:1108	Finally, electrochemical measurements confirmed that the coatings do not influence the corrosion susceptibility of AISI 316LVM stainless steel.					
31887952	4	44	theme	cell	779:782	arg1	viability					784:792	cell viability	779:792	cell viability improvement between 7% and 17%	779:823	Biocompatibility tests performed on human osteoblast cells, showed cell viability improvement between 7% and 17% compared to the control sample.					
31887952	2	45	theme	Cells	404:408	arg1	System					419:424	an Automated Transdermal Diffusion Cells Sampling System	369:424	an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method	369:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	6	46	theme	stainless	1199:1207	arg1	steel					1209:1213	AISI 316LVM stainless steel	1187:1213	AISI 316LVM stainless steel	1187:1213	Finally, electrochemical measurements confirmed that the coatings do not influence the corrosion susceptibility of AISI 316LVM stainless steel.					
31887952	0	47	theme	drug	84:87	arg1	delivery					89:96	controlled drug delivery	73:96	controlled drug delivery	73:96	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI 316LVM for controlled drug delivery, and improved osteogenic potential.					
31887952	1	48	dep	drug	275:278	arg1	cellulose					320:328	carboxymethyl cellulose	306:328	carboxymethyl cellulose	306:328	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	1	48	dep	drug	275:278	arg1	diclofenac					280:289	diclofenac	280:289	diclofenac	280:289	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
31887952	4	49	theme	control	841:847	arg1	sample					849:854	the control sample	837:854	the control sample	837:854	Biocompatibility tests performed on human osteoblast cells, showed cell viability improvement between 7% and 17% compared to the control sample.					
31887952	2	50	theme	Sampling	410:417	arg1	System					419:424	an Automated Transdermal Diffusion Cells Sampling System	369:424	an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method	369:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	0	51	theme	controlled	73:82	arg1	delivery					89:96	controlled drug delivery	73:96	controlled drug delivery	73:96	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI 316LVM for controlled drug delivery, and improved osteogenic potential.					
31887952	0	52	from	coatings	45:52	arg1	AISI					57:60	AISI	57:60	AISI	57:60	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI 316LVM for controlled drug delivery, and improved osteogenic potential.					
31887952	2	53	theme	released	473:480	arg1	method					515:520	the released drug concentration determination method	469:520	the released drug concentration determination method	469:520	Drug release testing was performed on an Automated Transdermal Diffusion Cells Sampling System in combination with UV-VIS spectroscopy as the released drug concentration determination method.					
31887952	0	54	theme	cellulose/diclofenac	14:33	arg1	coatings					45:52	Carboxymethyl cellulose/diclofenac bioactive coatings	0:52	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI	0:60	Carboxymethyl cellulose/diclofenac bioactive coatings on AISI 316LVM for controlled drug delivery, and improved osteogenic potential.					
31887952	1	55	theme	non-steroid	245:255	arg1	drug					275:278	the non-steroid anti-inflammatory drug	241:278	the non-steroid anti-inflammatory drug	241:278	This work reports on the preparation and systematic testing of a novel multi-layered coating, comprised of the non-steroid anti-inflammatory drug diclofenac and biopolymer carboxymethyl cellulose.					
30291886	6	0	theme	Bgn	825:827	arg1	treatment					835:843	Bgn siRNA treatment	825:843	Bgn siRNA treatment	825:843	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	5	1	theme	hippocampal	794:804	arg1	cultures					815:822	primary hippocampal neuronal cultures	786:822	primary hippocampal neuronal cultures	786:822	Fructose also reduced the levels of CREB and BDNF in primary hippocampal neuronal cultures.					
30291886	2	2	from	position	264:271	arg1	network					278:284	a network	276:284	a network of genes	276:293	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	7	3	theme	Bgn0/	1106:1110	arg1	mice					1112:1115	the Bgn0/ mice	1102:1115	the Bgn0/ mice	1102:1115	In addition, fructose consumption perturbed the systemic metabolism of glucose and lipids, that were also altered in the Bgn0/ mice.					
30291886	4	4	from	action	686:691	arg1	levels					725:730	hippocampal CREB levels	708:730	hippocampal CREB levels	708:730	These results were consistent with an attenuation of the action of fructose on hippocampal CREB levels.					
30291886	5	5	theme	neuronal	806:813	arg1	cultures					815:822	primary hippocampal neuronal cultures	786:822	primary hippocampal neuronal cultures	786:822	Fructose also reduced the levels of CREB and BDNF in primary hippocampal neuronal cultures.					
30291886	3	6	dep	learning	571:578	arg1	performance					591:601	performance	591:601	performance	591:601	We exposed male biglycan knockout mice (Bgn0/-) to fructose for 7 weeks, and results showed that Bgn0/- mice compensated for a decrement in learning and memory performance when exposed to fructose.					
30291886	6	7	from	reduction	928:936	arg1	increase					960:967	a fructose-related increase	941:967	a fructose-related increase in Bgn protein	941:982	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	7	8	theme	fructose	998:1005	arg1	consumption					1007:1017	fructose consumption	998:1017	fructose consumption	998:1017	In addition, fructose consumption perturbed the systemic metabolism of glucose and lipids, that were also altered in the Bgn0/ mice.					
30291886	6	9	from	increase	960:967	arg1	protein					976:982	Bgn protein	972:982	Bgn protein	972:982	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	2	10	theme	central	256:262	arg1	position					264:271	a central position	254:271	a central position in a network of genes	254:293	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	6	11	theme	BDNF	893:896	arg1	levels					898:903	CREB and BDNF levels	884:903	CREB and BDNF levels	884:903	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	8	12	theme	key	1225:1227	arg1	pathways					1239:1246	key molecular pathways	1225:1246	key molecular pathways involved in metabolism, immune response, and neuronal plasticity	1225:1311	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	1	13	theme	Dietary	66:72	arg1	fructose					74:81	Dietary fructose	66:81	Dietary fructose	66:81	Dietary fructose is a major contributor to the epidemic of diabetes and obesity, and it is an excellent model to study metabolic syndrome.					
30291886	1	13	theme	Dietary	66:72	arg1	contributor					94:104	a major contributor	86:104	a major contributor to the epidemic of diabetes and obesity	86:144	Dietary fructose is a major contributor to the epidemic of diabetes and obesity, and it is an excellent model to study metabolic syndrome.					
30291886	2	14	from	action	393:398	arg1	body					425:428	body	425:428	body	425:428	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	2	14	from	action	393:398	arg1	brain					415:419	brain	415:419	brain	415:419	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	7	15	theme	lipids	1068:1073	arg1	metabolism					1042:1051	the systemic metabolism	1029:1051	the systemic metabolism	1029:1051	In addition, fructose consumption perturbed the systemic metabolism of glucose and lipids, that were also altered in the Bgn0/ mice.					
30291886	4	16	from	attenuation	667:677	arg1	levels					725:730	hippocampal CREB levels	708:730	hippocampal CREB levels	708:730	These results were consistent with an attenuation of the action of fructose on hippocampal CREB levels.					
30291886	3	17	theme	knockout	456:463	arg1	mice					465:468	male biglycan knockout mice	442:468	male biglycan knockout mice (Bgn0/-)	442:477	We exposed male biglycan knockout mice (Bgn0/-) to fructose for 7 weeks, and results showed that Bgn0/- mice compensated for a decrement in learning and memory performance when exposed to fructose.					
30291886	9	18	theme	tissue-specific	1345:1359	arg1	role					1361:1364	the tissue-specific role	1341:1364	the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function	1341:1443	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	3	19	from	decrement	558:566	arg1	learning					571:578	learning	571:578	learning	571:578	We exposed male biglycan knockout mice (Bgn0/-) to fructose for 7 weeks, and results showed that Bgn0/- mice compensated for a decrement in learning and memory performance when exposed to fructose.					
30291886	3	19	from	decrement	558:566	arg1	memory					584:589	memory	584:589	memory	584:589	We exposed male biglycan knockout mice (Bgn0/-) to fructose for 7 weeks, and results showed that Bgn0/- mice compensated for a decrement in learning and memory performance when exposed to fructose.					
30291886	9	20	theme	fructose	1499:1506	arg1	impact					1489:1494	the impact	1485:1494	the impact of fructose across body and brain	1485:1528	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	5	21	theme	CREB	769:772	arg1	levels					759:764	the levels	755:764	the levels of CREB and BDNF in primary hippocampal neuronal cultures	755:822	Fructose also reduced the levels of CREB and BDNF in primary hippocampal neuronal cultures.					
30291886	9	22	theme	brain	1430:1434	arg1	regulation					1401:1410	the regulation	1397:1410	the regulation of metabolism and brain function	1397:1443	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	9	23	from	role	1361:1364	arg1	regulation					1401:1410	the regulation	1397:1410	the regulation of metabolism and brain function	1397:1443	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	2	24	from	brain	302:306	arg1	response					311:318	response	311:318	response to fructose consumption	311:342	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	8	25	theme	hypothalamus	1146:1157	arg1	profiling					1133:1141	Transcriptomic profiling	1118:1141	Transcriptomic profiling of hypothalamus, hippocampus, and liver	1118:1181	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	0	26	theme	Biglycan	0:7	arg1	gene					9:12	Biglycan gene	0:12	Biglycan gene	0:12	Biglycan gene connects metabolic dysfunction with brain disorder.					
30291886	6	27	theme	CREB	884:887	arg1	levels					898:903	CREB and BDNF levels	884:903	CREB and BDNF levels	884:903	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	9	28	theme	key	1467:1469	arg1	Bgn					1458:1460	Bgn	1458:1460	Bgn	1458:1460	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	9	28	theme	key	1467:1469	arg1	modulator					1471:1479	a key modulator	1465:1479	a key modulator for the impact of fructose across body and brain	1465:1528	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	1	29	theme	major	88:92	arg1	fructose					74:81	Dietary fructose	66:81	Dietary fructose	66:81	Dietary fructose is a major contributor to the epidemic of diabetes and obesity, and it is an excellent model to study metabolic syndrome.					
30291886	1	29	theme	major	88:92	arg1	contributor					94:104	a major contributor	86:104	a major contributor to the epidemic of diabetes and obesity	86:144	Dietary fructose is a major contributor to the epidemic of diabetes and obesity, and it is an excellent model to study metabolic syndrome.					
30291886	2	30	theme	fructose	323:330	arg1	consumption					332:342	fructose consumption	323:342	fructose consumption	323:342	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	0	31	theme	metabolic	23:31	arg1	dysfunction					33:43	metabolic dysfunction	23:43	metabolic dysfunction	23:43	Biglycan gene connects metabolic dysfunction with brain disorder.					
30291886	8	32	theme	regulatory	1197:1206	arg1	action					1208:1213	the regulatory action	1193:1213	the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity	1193:1311	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	8	33	from	action	1208:1213	arg1	pathways					1239:1246	key molecular pathways	1225:1246	key molecular pathways involved in metabolism, immune response, and neuronal plasticity	1225:1311	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	8	34	theme	immune	1272:1277	arg1	response					1279:1286	immune response	1272:1286	immune response	1272:1286	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	6	35	theme	fructose-related	943:958	arg1	increase					960:967	a fructose-related increase	941:967	a fructose-related increase in Bgn protein	941:982	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	2	36	theme	Bgn	373:375	arg1	capacity					361:368	the capacity	357:368	the capacity of Bgn to modulate the action of fructose on brain and body	357:428	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	6	37	theme	fructose	872:879	arg1	effects					861:867	these effects	855:867	these effects of fructose on CREB and BDNF levels	855:903	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	4	38	theme	action	686:691	arg1	attenuation					667:677	an attenuation	664:677	an attenuation of the action of fructose on hippocampal CREB levels	664:730	These results were consistent with an attenuation of the action of fructose on hippocampal CREB levels.					
30291886	8	39	theme	Bgn	1218:1220	arg1	action					1208:1213	the regulatory action	1193:1213	the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity	1193:1311	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	9	40	theme	metabolism	1415:1424	arg1	regulation					1401:1410	the regulation	1397:1410	the regulation of metabolism and brain function	1397:1443	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	8	41	theme	liver	1177:1181	arg1	profiling					1133:1141	Transcriptomic profiling	1118:1141	Transcriptomic profiling of hypothalamus, hippocampus, and liver	1118:1181	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	6	42	with	conjunction	909:919	arg1	reduction					928:936	a reduction	926:936	a reduction in a fructose-related increase in Bgn protein	926:982	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	1	43	theme	excellent	160:168	arg1	it					151:152	it	151:152	it	151:152	Dietary fructose is a major contributor to the epidemic of diabetes and obesity, and it is an excellent model to study metabolic syndrome.					
30291886	1	43	theme	excellent	160:168	arg1	model					170:174	an excellent model	157:174	an excellent model to study metabolic syndrome	157:202	Dietary fructose is a major contributor to the epidemic of diabetes and obesity, and it is an excellent model to study metabolic syndrome.					
30291886	9	44	theme	extracellular	1373:1385	arg1	matrix					1387:1392	the extracellular matrix	1369:1392	the extracellular matrix	1369:1392	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	2	45	theme	fructose	403:410	arg1	action					393:398	the action	389:398	the action of fructose on brain and body	389:428	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	7	46	theme	glucose	1056:1062	arg1	metabolism					1042:1051	the systemic metabolism	1029:1051	the systemic metabolism	1029:1051	In addition, fructose consumption perturbed the systemic metabolism of glucose and lipids, that were also altered in the Bgn0/ mice.					
30291886	4	47	theme	CREB	720:723	arg1	levels					725:730	hippocampal CREB levels	708:730	hippocampal CREB levels	708:730	These results were consistent with an attenuation of the action of fructose on hippocampal CREB levels.					
30291886	1	48	theme	metabolic	185:193	arg1	syndrome					195:202	metabolic syndrome	185:202	metabolic syndrome	185:202	Dietary fructose is a major contributor to the epidemic of diabetes and obesity, and it is an excellent model to study metabolic syndrome.					
30291886	7	49	theme	systemic	1033:1040	arg1	metabolism					1042:1051	the systemic metabolism	1029:1051	the systemic metabolism	1029:1051	In addition, fructose consumption perturbed the systemic metabolism of glucose and lipids, that were also altered in the Bgn0/ mice.					
30291886	8	50	theme	neuronal	1293:1300	arg1	plasticity					1302:1311	neuronal plasticity	1293:1311	neuronal plasticity	1293:1311	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	5	51	from	levels	759:764	arg1	cultures					815:822	primary hippocampal neuronal cultures	786:822	primary hippocampal neuronal cultures	786:822	Fructose also reduced the levels of CREB and BDNF in primary hippocampal neuronal cultures.					
30291886	0	52	theme	brain	50:54	arg1	disorder					56:63	brain disorder	50:63	brain disorder	50:63	Biglycan gene connects metabolic dysfunction with brain disorder.					
30291886	4	53	with	consistent	648:657	arg1	attenuation					667:677	an attenuation	664:677	an attenuation of the action of fructose on hippocampal CREB levels	664:730	These results were consistent with an attenuation of the action of fructose on hippocampal CREB levels.					
30291886	1	54	theme	obesity	138:144	arg1	epidemic					113:120	epidemic	113:120	epidemic	113:120	Dietary fructose is a major contributor to the epidemic of diabetes and obesity, and it is an excellent model to study metabolic syndrome.					
30291886	4	55	from	levels	725:730	arg1	attenuation					667:677	an attenuation	664:677	an attenuation of the action of fructose on hippocampal CREB levels	664:730	These results were consistent with an attenuation of the action of fructose on hippocampal CREB levels.					
30291886	3	56	dep	mice	465:468	arg1	Bgn0/-					471:476	Bgn0/-	471:476	Bgn0/-	471:476	We exposed male biglycan knockout mice (Bgn0/-) to fructose for 7 weeks, and results showed that Bgn0/- mice compensated for a decrement in learning and memory performance when exposed to fructose.					
30291886	9	57	dep	metabolism	1415:1424	arg1	function					1436:1443	function	1436:1443	function	1436:1443	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	6	58	from	effects	861:867	arg1	levels					898:903	CREB and BDNF levels	884:903	CREB and BDNF levels	884:903	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	3	59	theme	Bgn0/-	528:533	arg1	mice					535:538	Bgn0/- mice	528:538	Bgn0/- mice	528:538	We exposed male biglycan knockout mice (Bgn0/-) to fructose for 7 weeks, and results showed that Bgn0/- mice compensated for a decrement in learning and memory performance when exposed to fructose.					
30291886	2	60	theme	Bgn	236:238	arg1	gene					240:243	Bgn gene	236:243	Bgn gene	236:243	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	2	61	theme	genes	289:293	arg1	network					278:284	a network	276:284	a network of genes	276:293	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	5	62	theme	BDNF	778:781	arg1	levels					759:764	the levels	755:764	the levels of CREB and BDNF in primary hippocampal neuronal cultures	755:822	Fructose also reduced the levels of CREB and BDNF in primary hippocampal neuronal cultures.					
30291886	8	63	theme	Transcriptomic	1118:1131	arg1	profiling					1133:1141	Transcriptomic profiling	1118:1141	Transcriptomic profiling of hypothalamus, hippocampus, and liver	1118:1181	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	8	64	theme	molecular	1229:1237	arg1	pathways					1239:1246	key molecular pathways	1225:1246	key molecular pathways involved in metabolism, immune response, and neuronal plasticity	1225:1311	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	1	65	theme	diabetes	125:132	arg1	epidemic					113:120	epidemic	113:120	epidemic	113:120	Dietary fructose is a major contributor to the epidemic of diabetes and obesity, and it is an excellent model to study metabolic syndrome.					
30291886	4	66	theme	fructose	696:703	arg1	action					686:691	the action	682:691	the action of fructose on hippocampal CREB levels	682:730	These results were consistent with an attenuation of the action of fructose on hippocampal CREB levels.					
30291886	9	67	theme	matrix	1387:1392	arg1	role					1361:1364	the tissue-specific role	1341:1364	the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function	1341:1443	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	5	68	theme	primary	786:792	arg1	cultures					815:822	primary hippocampal neuronal cultures	786:822	primary hippocampal neuronal cultures	786:822	Fructose also reduced the levels of CREB and BDNF in primary hippocampal neuronal cultures.					
30291886	4	69	theme	hippocampal	708:718	arg1	levels					725:730	hippocampal CREB levels	708:730	hippocampal CREB levels	708:730	These results were consistent with an attenuation of the action of fructose on hippocampal CREB levels.					
30291886	8	70	theme	hippocampus	1160:1170	arg1	profiling					1133:1141	Transcriptomic profiling	1118:1141	Transcriptomic profiling of hypothalamus, hippocampus, and liver	1118:1181	Transcriptomic profiling of hypothalamus, hippocampus, and liver supported the regulatory action of Bgn on key molecular pathways involved in metabolism, immune response, and neuronal plasticity.					
30291886	3	71	theme	male	442:445	arg1	mice					465:468	male biglycan knockout mice	442:468	male biglycan knockout mice (Bgn0/-)	442:477	We exposed male biglycan knockout mice (Bgn0/-) to fructose for 7 weeks, and results showed that Bgn0/- mice compensated for a decrement in learning and memory performance when exposed to fructose.					
30291886	6	72	theme	Bgn	972:974	arg1	protein					976:982	Bgn protein	972:982	Bgn protein	972:982	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	9	73	theme	Overall	1314:1320	arg1	results					1322:1328	Overall results	1314:1328	Overall results	1314:1328	Overall results underscore the tissue-specific role of the extracellular matrix in the regulation of metabolism and brain function, and support Bgn as a key modulator for the impact of fructose across body and brain.					
30291886	6	74	theme	siRNA	829:833	arg1	treatment					835:843	Bgn siRNA treatment	825:843	Bgn siRNA treatment	825:843	Bgn siRNA treatment abolished these effects of fructose on CREB and BDNF levels, in conjunction with a reduction in a fructose-related increase in Bgn protein.					
30291886	2	75	theme	previous	214:221	arg1	studies					223:229	previous studies	214:229	previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption	214:342	Based on previous studies that Bgn gene occupies a central position in a network of genes in the brain in response to fructose consumption, we assessed the capacity of Bgn to modulate the action of fructose on brain and body.					
30291886	3	76	theme	biglycan	447:454	arg1	mice					465:468	male biglycan knockout mice	442:468	male biglycan knockout mice (Bgn0/-)	442:477	We exposed male biglycan knockout mice (Bgn0/-) to fructose for 7 weeks, and results showed that Bgn0/- mice compensated for a decrement in learning and memory performance when exposed to fructose.					
31615981	6	0	theme	MYB	843:845	arg1	factors					861:867	MYB transcription factors	843:867	MYB transcription factors	843:867	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	4	1	theme	factor	607:612	arg1	gene					624:627	the R2R3-MYB transcription factor VIRESCENS gene	580:627	the R2R3-MYB transcription factor VIRESCENS gene	580:627	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	3	2	from	length	399:404	arg1	772.3 Mb					387:394	772.3 Mb	387:394	772.3 Mb	387:394	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	1	3	theme	regions	128:134	arg1	palms					64:68	Date palms	59:68	Date palms (Phoenix dactylifera)	59:90	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	1	3	theme	regions	128:134	arg1	crop					115:118	an important fruit crop	96:118	an important fruit crop of arid regions of the Middle East and North Africa	96:170	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	6	4	theme	parallel	983:990	arg1	evolution					992:1000	parallel evolution	983:1000	parallel evolution	983:1000	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	5	5	theme	linked	819:824	arg1	genes					836:840	multiple linked invertase genes	810:840	multiple linked invertase genes	810:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	4	6	theme	transcription	593:605	arg1	factor					607:612	R2R3-MYB transcription factor	584:612	the R2R3-MYB transcription factor VIRESCENS gene	580:627	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	4	7	theme	start	706:710	arg1	mutation					718:725	start codon mutation	706:725	start codon mutation	706:725	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	3	8	theme	association	472:482	arg1	GWAS					493:496	GWAS	493:496	GWAS	493:496	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	3	8	theme	association	472:482	arg1	studies					484:490	genome-wide association studies	460:490	genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits	460:547	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	6	9	theme	sugar	917:921	arg1	composition					923:933	sugar composition	917:933	sugar composition	917:933	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	1	10	theme	Date	59:62	arg1	palms					64:68	Date palms	59:68	Date palms (Phoenix dactylifera)	59:90	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	1	10	theme	Date	59:62	arg1	dactylifera					79:89	dactylifera	79:89	dactylifera	79:89	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	1	10	theme	Date	59:62	arg1	crop					115:118	an important fruit crop	96:118	an important fruit crop of arid regions of the Middle East and North Africa	96:170	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	5	11	theme	composition	763:773	arg1	polymorphisms					793:805	sugar composition spanning deletion polymorphisms	757:805	sugar composition spanning deletion polymorphisms in multiple linked invertase genes	757:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	6	12	theme	evolutionary	1009:1020	arg1	diversification					1022:1036	the evolutionary diversification	1005:1036	the evolutionary diversification of domesticated species	1005:1060	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	5	13	theme	deletion	784:791	arg1	polymorphisms					793:805	sugar composition spanning deletion polymorphisms	757:805	sugar composition spanning deletion polymorphisms in multiple linked invertase genes	757:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	4	14	theme	color	566:570	arg1	GWAS					572:575	a fruit color GWAS	558:575	a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene	558:627	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	5	15	theme	invertase	826:834	arg1	genes					836:840	multiple linked invertase genes	810:840	multiple linked invertase genes	810:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	5	16	theme	GWAS	743:746	arg1	peak					748:751	a GWAS peak	741:751	a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes	741:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	6	17	from	importance	969:978	arg1	diversification					1022:1036	the evolutionary diversification	1005:1036	the evolutionary diversification of domesticated species	1005:1060	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	2	18	theme	date	229:232	arg1	palms					234:238	date palms	229:238	date palms	229:238	Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species.					
31615981	4	19	theme	fruit	560:564	arg1	GWAS					572:575	a fruit color GWAS	558:575	a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene	558:627	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	0	20	theme	association	12:22	arg1	mapping					24:30	Genome-wide association mapping	0:30	Genome-wide association mapping of date palm fruit	0:49	Genome-wide association mapping of date palm fruit traits.					
31615981	1	21	theme	East	150:153	arg1	regions					128:134	arid regions	123:134	arid regions of the Middle East and North Africa	123:170	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	0	22	theme	Genome-wide	0:10	arg1	mapping					24:30	Genome-wide association mapping	0:30	Genome-wide association mapping of date palm fruit	0:49	Genome-wide association mapping of date palm fruit traits.					
31615981	6	23	from	composition	923:933	arg1	crops					944:948	other crops	938:948	other crops	938:948	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	4	24	theme	functional	642:651	arg1	alleles					653:659	functional alleles	642:659	functional alleles that include a retrotransposon insertion and start codon mutation	642:725	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	4	25	theme	retrotransposon	676:690	arg1	insertion					692:700	a retrotransposon insertion	674:700	a retrotransposon insertion	674:700	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	5	26	from	polymorphisms	793:805	arg1	genes					836:840	multiple linked invertase genes	810:840	multiple linked invertase genes	810:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	3	27	theme	improved	325:332	arg1	assembly					351:358	an improved long-read genome assembly	322:358	an improved long-read genome assembly	322:358	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	6	28	theme	fruit	901:905	arg1	color					907:911	fruit color	901:911	fruit color	901:911	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	5	29	link	linked	819:824	arg1	genes					836:840	multiple linked invertase genes	810:840	multiple linked invertase genes	810:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	2	30	theme	genomic	264:270	arg1	studies					272:278	evolutionary genomic studies	251:278	evolutionary genomic studies of this perennial species	251:304	Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species.					
31615981	3	31	theme	determining	510:520	arg1	region					522:527	the sex determining region	502:527	the sex determining region	502:527	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	3	32	theme	contig	412:417	arg1	N50					419:421	contig N50	412:421	contig N50 of 897.2 Kb	412:433	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	3	33	theme	long-read	334:342	arg1	assembly					351:358	an improved long-read genome assembly	322:358	an improved long-read genome assembly	322:358	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	6	34	theme	domesticated	1041:1052	arg1	species					1054:1060	domesticated species	1041:1060	domesticated species	1041:1060	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	6	35	theme	other	938:942	arg1	crops					944:948	other crops	938:948	other crops	938:948	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	2	36	theme	evolutionary	251:262	arg1	studies					272:278	evolutionary genomic studies	251:278	evolutionary genomic studies of this perennial species	251:304	Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species.					
31615981	1	37	theme	Africa	165:170	arg1	regions					128:134	arid regions	123:134	arid regions of the Middle East and North Africa	123:170	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	0	38	theme	palm	40:43	arg1	fruit					45:49	date palm fruit	35:49	date palm fruit	35:49	Genome-wide association mapping of date palm fruit traits.					
31615981	2	39	theme	species	298:304	arg1	studies					272:278	evolutionary genomic studies	251:278	evolutionary genomic studies of this perennial species	251:304	Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species.					
31615981	6	40	from	color	907:911	arg1	crops					944:948	other crops	938:948	other crops	938:948	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	2	41	theme	few	197:199	arg1	resources					209:217	few genomic resources	197:217	few genomic resources	197:217	Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species.					
31615981	6	42	theme	evolution	992:1000	arg1	importance					969:978	the importance	965:978	the importance of parallel evolution in the evolutionary diversification of domesticated species	965:1060	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	0	43	theme	date	35:38	arg1	fruit					45:49	date palm fruit	35:49	date palm fruit	35:49	Genome-wide association mapping of date palm fruit traits.					
31615981	3	44	theme	region	522:527	arg1	GWAS					493:496	GWAS	493:496	GWAS	493:496	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	3	44	theme	region	522:527	arg1	studies					484:490	genome-wide association studies	460:490	genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits	460:547	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	5	45	theme	sugar	757:761	arg1	polymorphisms					793:805	sugar composition spanning deletion polymorphisms	757:805	sugar composition spanning deletion polymorphisms in multiple linked invertase genes	757:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	6	46	theme	species	1054:1060	arg1	diversification					1022:1036	the evolutionary diversification	1005:1036	the evolutionary diversification of domesticated species	1005:1060	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	3	47	theme	Kb	432:433	arg1	N50					419:421	contig N50	412:421	contig N50 of 897.2 Kb	412:433	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	0	48	theme	fruit	45:49	arg1	mapping					24:30	Genome-wide association mapping	0:30	Genome-wide association mapping of date palm fruit	0:49	Genome-wide association mapping of date palm fruit traits.					
31615981	3	49	with	772.3 Mb	387:394	arg1	N50					419:421	contig N50	412:421	contig N50 of 897.2 Kb	412:433	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	3	50	theme	sex	506:508	arg1	region					522:527	the sex determining region	502:527	the sex determining region	502:527	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	1	51	theme	important	99:107	arg1	palms					64:68	Date palms	59:68	Date palms (Phoenix dactylifera)	59:90	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	1	51	theme	important	99:107	arg1	crop					115:118	an important fruit crop	96:118	an important fruit crop of arid regions of the Middle East and North Africa	96:170	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	4	52	theme	codon	712:716	arg1	mutation					718:725	start codon mutation	706:725	start codon mutation	706:725	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	3	53	theme	fruit	536:540	arg1	traits					542:547	21 fruit traits	533:547	21 fruit traits	533:547	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	1	54	theme	fruit	109:113	arg1	palms					64:68	Date palms	59:68	Date palms (Phoenix dactylifera)	59:90	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	1	54	theme	fruit	109:113	arg1	crop					115:118	an important fruit crop	96:118	an important fruit crop of arid regions of the Middle East and North Africa	96:170	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	3	55	theme	genome-wide	460:470	arg1	GWAS					493:496	GWAS	493:496	GWAS	493:496	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	3	55	theme	genome-wide	460:470	arg1	studies					484:490	genome-wide association studies	460:490	genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits	460:547	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	3	56	theme	traits	542:547	arg1	GWAS					493:496	GWAS	493:496	GWAS	493:496	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	3	56	theme	traits	542:547	arg1	studies					484:490	genome-wide association studies	460:490	genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits	460:547	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	1	57	theme	arid	123:126	arg1	regions					128:134	arid regions	123:134	arid regions of the Middle East and North Africa	123:170	Date palms (Phoenix dactylifera) are an important fruit crop of arid regions of the Middle East and North Africa.					
31615981	4	58	from	gene	624:627	arg1	GWAS					572:575	a fruit color GWAS	558:575	a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene	558:627	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	2	59	theme	perennial	288:296	arg1	species					298:304	this perennial species	283:304	this perennial species	283:304	Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species.					
31615981	5	60	theme	multiple	810:817	arg1	genes					836:840	multiple linked invertase genes	810:840	multiple linked invertase genes	810:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	3	61	from	772.3 Mb	387:394	arg1	length					399:404	length	399:404	length	399:404	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	4	62	theme	R2R3-MYB	584:591	arg1	factor					607:612	R2R3-MYB transcription factor	584:612	the R2R3-MYB transcription factor VIRESCENS gene	580:627	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
31615981	5	63	theme	spanning	775:782	arg1	polymorphisms					793:805	sugar composition spanning deletion polymorphisms	757:805	sugar composition spanning deletion polymorphisms in multiple linked invertase genes	757:840	We also find a GWAS peak for sugar composition spanning deletion polymorphisms in multiple linked invertase genes.					
31615981	6	64	theme	transcription	847:859	arg1	factors					861:867	MYB transcription factors	843:867	MYB transcription factors	843:867	MYB transcription factors and invertase are implicated in fruit color and sugar composition in other crops, demonstrating the importance of parallel evolution in the evolutionary diversification of domesticated species.					
31615981	2	65	theme	genomic	201:207	arg1	resources					209:217	few genomic resources	197:217	few genomic resources	197:217	Despite its importance, few genomic resources exist for date palms, hampering evolutionary genomic studies of this perennial species.					
31615981	3	66	theme	genome	344:349	arg1	assembly					351:358	an improved long-read genome assembly	322:358	an improved long-read genome assembly	322:358	Here we report an improved long-read genome assembly for P. dactylifera that is 772.3 Mb in length, with contig N50 of 897.2 Kb, and use this to perform genome-wide association studies (GWAS) of the sex determining region and 21 fruit traits.					
31615981	4	67	theme	VIRESCENS	614:622	arg1	gene					624:627	the R2R3-MYB transcription factor VIRESCENS gene	580:627	the R2R3-MYB transcription factor VIRESCENS gene	580:627	We find a fruit color GWAS at the R2R3-MYB transcription factor VIRESCENS gene and identify functional alleles that include a retrotransposon insertion and start codon mutation.					
30774006	1	0	theme	mulberry	314:321	arg1	supplementation					323:337	mulberry supplementation	314:337	mulberry supplementation	314:337	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	2	1	theme	BALB/c	345:350	arg1	mice					352:355	Male BALB/c mice	340:355	Male BALB/c mice	340:355	Male BALB/c mice received a diet supplemented with mulberry juice freeze-dried powder (MFP) or not for 3 weeks.					
30774006	3	2	contain	containing	491:500	arg2	w/v					506:508	w/v	506:508	w/v	506:508	After 3 weeks, the mice received water containing 5% (w/v) DSS or not for 1 week.					
30774006	3	2	contain	containing	491:500	arg1	water					485:489	water	485:489	water containing 5% (w/v)	485:509	After 3 weeks, the mice received water containing 5% (w/v) DSS or not for 1 week.					
30774006	3	2	contain	containing	491:500	arg2	%					503:503	5%	502:503	5% (w/v)	502:509	After 3 weeks, the mice received water containing 5% (w/v) DSS or not for 1 week.					
30774006	2	3	theme	Male	340:343	arg1	mice					352:355	Male BALB/c mice	340:355	Male BALB/c mice	340:355	Male BALB/c mice received a diet supplemented with mulberry juice freeze-dried powder (MFP) or not for 3 weeks.					
30774006	8	4	theme	gut	963:965	arg1	flora					977:981	the gut microbial flora	959:981	the gut microbial flora	959:981	These results may indicate that mulberry mitigates DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions.					
30774006	0	5	theme	colon	89:93	arg1	mucosa					95:100	colon mucosa	89:100	colon mucosa	89:100	Mulberry juice freeze-dried powder attenuates the disease severity by the maintaining of colon mucosa in mice with DSS-induced acute colitis.					
30774006	8	6	dep	changing	950:957	arg1	a					948:948	a	948:948	a	948:948	These results may indicate that mulberry mitigates DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions.					
30774006	8	7	dep	colitis	937:943	arg1	improving					990:998	improving	990:998	improving mucosal conditions	990:1017	These results may indicate that mulberry mitigates DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions.					
30774006	8	7	dep	colitis	937:943	arg1	changing					950:957	changing	950:957	a changing the gut microbial flora	948:981	These results may indicate that mulberry mitigates DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions.					
30774006	6	8	located	observed	704:711	arg2	subgroup					691:698	the Clostridium perfringens subgroup	663:698	the Clostridium perfringens subgroup	663:698	and the Clostridium perfringens subgroup was observed in mice not fed MFP.					
30774006	6	8	located	observed	704:711	arg1	mice					716:719	mice	716:719	mice not fed MFP	716:731	and the Clostridium perfringens subgroup was observed in mice not fed MFP.					
30774006	8	9	theme	microbial	967:975	arg1	flora					977:981	the gut microbial flora	959:981	the gut microbial flora	959:981	These results may indicate that mulberry mitigates DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions.					
30774006	0	10	with	mice	105:108	arg1	colitis					133:139	DSS-induced acute colitis	115:139	DSS-induced acute colitis	115:139	Mulberry juice freeze-dried powder attenuates the disease severity by the maintaining of colon mucosa in mice with DSS-induced acute colitis.					
30774006	0	11	theme	Mulberry	0:7	arg1	powder					28:33	Mulberry juice freeze-dried powder	0:33	Mulberry juice freeze-dried powder	0:33	Mulberry juice freeze-dried powder attenuates the disease severity by the maintaining of colon mucosa in mice with DSS-induced acute colitis.					
30774006	0	12	theme	juice	9:13	arg1	powder					28:33	Mulberry juice freeze-dried powder	0:33	Mulberry juice freeze-dried powder	0:33	Mulberry juice freeze-dried powder attenuates the disease severity by the maintaining of colon mucosa in mice with DSS-induced acute colitis.					
30774006	8	13	theme	DSS-induced	919:929	arg1	colitis					937:943	DSS-induced acute colitis	919:943	DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions	919:1017	These results may indicate that mulberry mitigates DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions.					
30774006	8	14	theme	mucosal	1000:1006	arg1	conditions					1008:1017	mucosal conditions	1000:1017	mucosal conditions	1000:1017	These results may indicate that mulberry mitigates DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions.					
30774006	1	15	theme	supplementation	323:337	arg1	colitis					288:294	dextran sodium sulfate (DSS)-induced acute colitis	245:294	dextran sodium sulfate (DSS)-induced acute colitis	245:294	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	1	15	theme	supplementation	323:337	arg1	effect					304:309	the effect	300:309	the effect of mulberry supplementation	300:337	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	7	16	theme	NLRP6	764:768	arg1	expression					770:779	NLRP6 expression	764:779	NLRP6 expression	764:779	The number of goblet cell and NLRP6 expression were observed in mice fed a diet supplemented with MFP compared with mice not fed MFP.					
30774006	1	17	theme	microbial	175:183	arg1	compositions					185:196	the microbial compositions	171:196	the microbial compositions	171:196	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	7	18	theme	goblet	748:753	arg1	cell					755:758	goblet cell	748:758	goblet cell	748:758	The number of goblet cell and NLRP6 expression were observed in mice fed a diet supplemented with MFP compared with mice not fed MFP.					
30774006	0	19	theme	DSS-induced	115:125	arg1	colitis					133:139	DSS-induced acute colitis	115:139	DSS-induced acute colitis	115:139	Mulberry juice freeze-dried powder attenuates the disease severity by the maintaining of colon mucosa in mice with DSS-induced acute colitis.					
30774006	7	20	theme	expression	770:779	arg1	number					738:743	The number	734:743	The number of goblet cell and NLRP6 expression	734:779	The number of goblet cell and NLRP6 expression were observed in mice fed a diet supplemented with MFP compared with mice not fed MFP.					
30774006	0	21	theme	freeze-dried	15:26	arg1	powder					28:33	Mulberry juice freeze-dried powder	0:33	Mulberry juice freeze-dried powder	0:33	Mulberry juice freeze-dried powder attenuates the disease severity by the maintaining of colon mucosa in mice with DSS-induced acute colitis.					
30774006	1	22	theme	-induced	273:280	arg1	colitis					288:294	dextran sodium sulfate (DSS)-induced acute colitis	245:294	dextran sodium sulfate (DSS)-induced acute colitis	245:294	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	7	23	theme	cell	755:758	arg1	number					738:743	The number	734:743	The number of goblet cell and NLRP6 expression	734:779	The number of goblet cell and NLRP6 expression were observed in mice fed a diet supplemented with MFP compared with mice not fed MFP.					
30774006	2	24	theme	freeze-dried	406:417	arg1	MFP					427:429	MFP	427:429	MFP	427:429	Male BALB/c mice received a diet supplemented with mulberry juice freeze-dried powder (MFP) or not for 3 weeks.					
30774006	2	24	theme	freeze-dried	406:417	arg1	powder					419:424	mulberry juice freeze-dried powder	391:424	mulberry juice freeze-dried powder (MFP)	391:430	Male BALB/c mice received a diet supplemented with mulberry juice freeze-dried powder (MFP) or not for 3 weeks.					
30774006	1	25	theme	acute	282:286	arg1	colitis					288:294	dextran sodium sulfate (DSS)-induced acute colitis	245:294	dextran sodium sulfate (DSS)-induced acute colitis	245:294	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	1	26	theme	gene	202:205	arg1	expression					207:216	gene expression	202:216	gene expression	202:216	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	2	27	theme	juice	400:404	arg1	MFP					427:429	MFP	427:429	MFP	427:429	Male BALB/c mice received a diet supplemented with mulberry juice freeze-dried powder (MFP) or not for 3 weeks.					
30774006	2	27	theme	juice	400:404	arg1	powder					419:424	mulberry juice freeze-dried powder	391:424	mulberry juice freeze-dried powder (MFP)	391:430	Male BALB/c mice received a diet supplemented with mulberry juice freeze-dried powder (MFP) or not for 3 weeks.					
30774006	0	28	theme	acute	127:131	arg1	colitis					133:139	DSS-induced acute colitis	115:139	DSS-induced acute colitis	115:139	Mulberry juice freeze-dried powder attenuates the disease severity by the maintaining of colon mucosa in mice with DSS-induced acute colitis.					
30774006	2	29	theme	mulberry	391:398	arg1	MFP					427:429	MFP	427:429	MFP	427:429	Male BALB/c mice received a diet supplemented with mulberry juice freeze-dried powder (MFP) or not for 3 weeks.					
30774006	2	29	theme	mulberry	391:398	arg1	powder					419:424	mulberry juice freeze-dried powder	391:424	mulberry juice freeze-dried powder (MFP)	391:430	Male BALB/c mice received a diet supplemented with mulberry juice freeze-dried powder (MFP) or not for 3 weeks.					
30774006	1	30	from	inflammation	229:240	arg1	colitis					288:294	dextran sodium sulfate (DSS)-induced acute colitis	245:294	dextran sodium sulfate (DSS)-induced acute colitis	245:294	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	1	30	from	inflammation	229:240	arg1	effect					304:309	the effect	300:309	the effect of mulberry supplementation	300:337	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	0	31	theme	disease	50:56	arg1	severity					58:65	the disease severity	46:65	the disease severity	46:65	Mulberry juice freeze-dried powder attenuates the disease severity by the maintaining of colon mucosa in mice with DSS-induced acute colitis.					
30774006	5	32	theme	significant	614:624	arg1	decrease					626:633	A significant decrease	612:633	A significant decrease in Bifidobacterium	612:652	A significant decrease in Bifidobacterium spp.					
30774006	6	33	dep	Clostridium	667:677	arg1	perfringens					679:689	perfringens	679:689	perfringens	679:689	and the Clostridium perfringens subgroup was observed in mice not fed MFP.					
30774006	7	34	located	observed	786:793	arg2	number					738:743	The number	734:743	The number of goblet cell and NLRP6 expression	734:779	The number of goblet cell and NLRP6 expression were observed in mice fed a diet supplemented with MFP compared with mice not fed MFP.					
30774006	7	34	located	observed	786:793	arg1	mice					798:801	mice	798:801	mice fed a diet supplemented with MFP compared with mice not fed MFP	798:865	The number of goblet cell and NLRP6 expression were observed in mice fed a diet supplemented with MFP compared with mice not fed MFP.					
30774006	8	35	theme	acute	931:935	arg1	colitis					937:943	DSS-induced acute colitis	919:943	DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions	919:1017	These results may indicate that mulberry mitigates DSS-induced acute colitis by a changing the gut microbial flora and by improving mucosal conditions.					
30774006	5	36	from	decrease	626:633	arg1	Bifidobacterium					638:652	Bifidobacterium	638:652	Bifidobacterium	638:652	A significant decrease in Bifidobacterium spp.					
30774006	1	37	theme	related	218:224	arg1	compositions					185:196	the microbial compositions	171:196	the microbial compositions	171:196	This study aimed to evaluate the microbial compositions and gene expression related to inflammation in dextran sodium sulfate (DSS)-induced acute colitis and the effect of mulberry supplementation.					
30774006	4	38	theme	disease	538:544	arg1	score					561:565	The disease activity index score	534:565	The disease activity index score in mice fed MFP	534:581	The disease activity index score in mice fed MFP was significantly decreased.					
30774006	4	39	from	score	561:565	arg1	mice					570:573	mice	570:573	mice fed MFP	570:581	The disease activity index score in mice fed MFP was significantly decreased.					
30774006	6	40	theme	Clostridium	667:677	arg1	subgroup					691:698	the Clostridium perfringens subgroup	663:698	the Clostridium perfringens subgroup	663:698	and the Clostridium perfringens subgroup was observed in mice not fed MFP.					
30774006	4	41	theme	activity	546:553	arg1	score					561:565	The disease activity index score	534:565	The disease activity index score in mice fed MFP	534:581	The disease activity index score in mice fed MFP was significantly decreased.					
30774006	4	42	theme	index	555:559	arg1	score					561:565	The disease activity index score	534:565	The disease activity index score in mice fed MFP	534:581	The disease activity index score in mice fed MFP was significantly decreased.					
31442510	7	0	theme	gasoil < mobil-1	1005:1020	arg1	oil					1058:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	5	1	theme	ButA	715:718	arg1	copolymer					721:729	NCS-g-poly (ButA) copolymer	703:729	NCS-g-poly (ButA) copolymer with an oil-contact angle 31°	703:759	Moreover, measurements of contact angle proved the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°.					
31442510	6	2	theme	oil	832:834	arg1	dose					836:839	oil dose	832:839	oil dose	832:839	Factors affecting the removal process such as contact time, oil type, oil dose, adsorbent dose, temperature and agitation speed were optimized.					
31442510	7	3	theme	oil < light	1022:1032	arg1	oil					1058:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	3	4	theme	grafted	457:463	arg1	copolymer					465:473	The grafted copolymer	453:473	The grafted copolymer	453:473	The grafted copolymer was characterized by FTIR, TGA and SEM tools.					
31442510	7	5	from	increment	909:917	arg1	%					939:939	%	939:939	%	939:939	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	7	5	from	increment	909:917	arg1	removal					930:936	the oil removal	922:936	the oil removal (%)	922:940	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	8	6	theme	25 °C.	1257:1262	arg1	parameters					1278:1287	25 °C. Thermodynamic parameters	1257:1287	25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous	1257:1383	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	8	7	theme	Va	1303:1304	arg1	plot					1314:1317	Van't Hoff plot	1303:1317	Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous	1303:1383	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	1	8	theme	low-cost	266:273	arg1	copolymer					236:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	1	8	theme	low-cost	266:273	arg1	oil-adsorbent					275:287	a low-cost oil-adsorbent	264:287	a low-cost oil-adsorbent	264:287	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	9	9	theme	petroleum	1471:1479	arg1	removal					1492:1498	petroleum oil spills removal	1471:1498	petroleum oil spills removal	1471:1498	The results nominate the superoleophilic adsorbent as a potential oil- adsorbent for petroleum oil spills removal.					
31442510	4	10	theme	grafting	525:532	arg1	percent					534:540	The grafting percent	521:540	The grafting percent	521:540	The grafting percent was augmented and reached 88.5% with increasing ButA concentration up to 156 mM.					
31442510	5	11	theme	superoleophilic	674:688	arg1	character					690:698	the superoleophilic character	670:698	the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°	670:759	Moreover, measurements of contact angle proved the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°.					
31442510	6	12	theme	oil	822:824	arg1	type					826:829	oil type	822:829	oil type	822:829	Factors affecting the removal process such as contact time, oil type, oil dose, adsorbent dose, temperature and agitation speed were optimized.					
31442510	9	13	theme	oil	1481:1483	arg1	removal					1492:1498	petroleum oil spills removal	1471:1498	petroleum oil spills removal	1471:1498	The results nominate the superoleophilic adsorbent as a potential oil- adsorbent for petroleum oil spills removal.					
31442510	2	14	theme	chitosan	363:370	arg1	base					385:388	nonanyl chitosan (NCS) schiff base	355:388	nonanyl chitosan (NCS) schiff base	355:388	Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl acrylate monomers (ButA).					
31442510	1	15	theme	novel	155:159	arg1	copolymer					236:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	1	15	theme	novel	155:159	arg1	oil-adsorbent					275:287	a low-cost oil-adsorbent	264:287	a low-cost oil-adsorbent	264:287	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	7	16	theme	oil < heavy	1040:1050	arg1	oil					1058:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	7	17	theme	crude	1052:1056	arg1	oil					1058:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	7	18	theme	crude	1034:1038	arg1	oil					1058:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	gasoil < mobil-1 oil < light crude oil < heavy crude oil	1005:1060	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	5	19	with	copolymer	721:729	arg1	31°					757:759	an oil-contact angle 31°	736:759	an oil-contact angle 31°	736:759	Moreover, measurements of contact angle proved the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°.					
31442510	1	20	theme	superoleophilic-hydrophobic	161:187	arg1	copolymer					236:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	1	20	theme	superoleophilic-hydrophobic	161:187	arg1	oil-adsorbent					275:287	a low-cost oil-adsorbent	264:287	a low-cost oil-adsorbent	264:287	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	5	21	theme	oil-contact	739:749	arg1	angle					751:755	an oil-contact angle	736:755	an oil-contact angle 31°	736:759	Moreover, measurements of contact angle proved the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°.					
31442510	7	22	theme	oil	975:977	arg1	viscosity					979:987	the oil viscosity	971:987	the oil viscosity	971:987	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	9	23	theme	spills	1485:1490	arg1	removal					1492:1498	petroleum oil spills removal	1471:1498	petroleum oil spills removal	1471:1498	The results nominate the superoleophilic adsorbent as a potential oil- adsorbent for petroleum oil spills removal.					
31442510	1	24	theme	nonanyl	189:195	arg1	copolymer					236:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	1	24	theme	nonanyl	189:195	arg1	oil-adsorbent					275:287	a low-cost oil-adsorbent	264:287	a low-cost oil-adsorbent	264:287	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	5	25	theme	angle	751:755	arg1	31°					757:759	an oil-contact angle 31°	736:759	an oil-contact angle 31°	736:759	Moreover, measurements of contact angle proved the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°.					
31442510	5	26	theme	contact	649:655	arg1	angle					657:661	contact angle	649:661	contact angle	649:661	Moreover, measurements of contact angle proved the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°.					
31442510	8	27	theme	equilibrium	1142:1152	arg1	data					1154:1157	the equilibrium data	1138:1157	the equilibrium data	1138:1157	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	2	28	theme	nonanyl	355:361	arg1	NCS					373:375	NCS	373:375	NCS	373:375	Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl acrylate monomers (ButA).					
31442510	2	28	theme	nonanyl	355:361	arg1	chitosan					363:370	nonanyl chitosan	355:370	nonanyl chitosan (NCS) schiff base	355:388	Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl acrylate monomers (ButA).					
31442510	0	29	theme	novel	17:21	arg1	acrylate					77:84	butyl acrylate	71:84	butyl acrylate	71:84	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	0	29	theme	novel	17:21	arg1	chitosan-poly					56:68	a novel low-cost superoleophilic nonanyl chitosan-poly	15:68	a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate)	15:85	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	8	30	theme	order	1122:1126	arg1	model					1128:1132	the pseudo-second order model	1104:1132	the pseudo-second order model	1104:1132	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	0	31	theme	adsorptive	113:122	arg1	removal					124:130	the adsorptive removal	109:130	the adsorptive removal of crude oil spills	109:150	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	6	32	theme	contact	808:814	arg1	time					816:819	contact time	808:819	contact time	808:819	Factors affecting the removal process such as contact time, oil type, oil dose, adsorbent dose, temperature and agitation speed were optimized.					
31442510	8	33	theme	capacity	1234:1241	arg1	108.79 g/g					1243:1252	a maximum adsorption capacity 108.79 g/g	1213:1252	a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous	1213:1383	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	7	34	theme	oil	1058:1060	arg1	order					996:1000	the order	992:1000	the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil	992:1060	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	1	35	theme	butyl	212:216	arg1	copolymer					236:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	1	35	theme	butyl	212:216	arg1	oil-adsorbent					275:287	a low-cost oil-adsorbent	264:287	a low-cost oil-adsorbent	264:287	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	0	36	theme	superoleophilic	32:46	arg1	acrylate					77:84	butyl acrylate	71:84	butyl acrylate	71:84	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	0	36	theme	superoleophilic	32:46	arg1	chitosan-poly					56:68	a novel low-cost superoleophilic nonanyl chitosan-poly	15:68	a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate)	15:85	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	0	37	theme	crude	135:139	arg1	spills					145:150	crude oil spills	135:150	crude oil spills	135:150	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	1	38	theme	acrylate	218:225	arg1	copolymer					236:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	1	38	theme	acrylate	218:225	arg1	oil-adsorbent					275:287	a low-cost oil-adsorbent	264:287	a low-cost oil-adsorbent	264:287	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	5	39	theme	angle	657:661	arg1	measurements					633:644	measurements	633:644	measurements of contact angle	633:661	Moreover, measurements of contact angle proved the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°.					
31442510	1	40	theme	chitosan-poly	197:209	arg1	copolymer					236:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	1	40	theme	chitosan-poly	197:209	arg1	oil-adsorbent					275:287	a low-cost oil-adsorbent	264:287	a low-cost oil-adsorbent	264:287	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	2	41	theme	schiff	378:383	arg1	base					385:388	nonanyl chitosan (NCS) schiff base	355:388	nonanyl chitosan (NCS) schiff base	355:388	Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl acrylate monomers (ButA).					
31442510	0	42	theme	low-cost	23:30	arg1	acrylate					77:84	butyl acrylate	71:84	butyl acrylate	71:84	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	0	42	theme	low-cost	23:30	arg1	chitosan-poly					56:68	a novel low-cost superoleophilic nonanyl chitosan-poly	15:68	a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate)	15:85	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	2	43	theme	acrylate	427:434	arg1	ButA					446:449	ButA	446:449	ButA	446:449	Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl acrylate monomers (ButA).					
31442510	2	43	theme	acrylate	427:434	arg1	monomers					436:443	butyl acrylate monomers	421:443	butyl acrylate monomers (ButA)	421:450	Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl acrylate monomers (ButA).					
31442510	8	44	theme	Hoff	1309:1312	arg1	plot					1314:1317	Van't Hoff plot	1303:1317	Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous	1303:1383	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	7	45	theme	oil	926:928	arg1	%					939:939	%	939:939	%	939:939	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	7	45	theme	oil	926:928	arg1	removal					930:936	the oil removal	922:936	the oil removal (%)	922:940	An increment in the oil removal (%) was observed with increasing the oil viscosity in the order of gasoil < mobil-1 oil < light crude oil < heavy crude oil.					
31442510	0	46	theme	chitosan-poly	56:68	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate)	0:85	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	0	47	theme	spills	145:150	arg1	removal					124:130	the adsorptive removal	109:130	the adsorptive removal of crude oil spills	109:150	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	5	48	theme	NCS-g-poly	703:712	arg1	copolymer					721:729	NCS-g-poly (ButA) copolymer	703:729	NCS-g-poly (ButA) copolymer with an oil-contact angle 31°	703:759	Moreover, measurements of contact angle proved the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°.					
31442510	0	49	theme	nonanyl	48:54	arg1	acrylate					77:84	butyl acrylate	71:84	butyl acrylate	71:84	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	0	49	theme	nonanyl	48:54	arg1	chitosan-poly					56:68	a novel low-cost superoleophilic nonanyl chitosan-poly	15:68	a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate)	15:85	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	9	50	theme	adsorbent	1457:1465	arg1	oil-					1452:1455	a potential oil-	1440:1455	a potential oil- adsorbent for petroleum oil spills removal	1440:1498	The results nominate the superoleophilic adsorbent as a potential oil- adsorbent for petroleum oil spills removal.					
31442510	0	51	theme	oil	141:143	arg1	spills					145:150	crude oil spills	135:150	crude oil spills	135:150	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	6	52	theme	removal	784:790	arg1	process					792:798	the removal process	780:798	the removal process such as contact time, oil type, oil dose, adsorbent dose, temperature and agitation speed	780:888	Factors affecting the removal process such as contact time, oil type, oil dose, adsorbent dose, temperature and agitation speed were optimized.					
31442510	8	53	theme	Langmuir	1193:1200	arg1	model					1202:1206	the Langmuir model	1189:1206	the Langmuir model	1189:1206	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	8	54	from	parameters	1278:1287	arg1	108.79 g/g					1243:1252	a maximum adsorption capacity 108.79 g/g	1213:1252	a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous	1213:1383	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	6	55	theme	adsorbent	842:850	arg1	dose					852:855	adsorbent dose	842:855	adsorbent dose	842:855	Factors affecting the removal process such as contact time, oil type, oil dose, adsorbent dose, temperature and agitation speed were optimized.					
31442510	3	56	dep	FTIR	496:499	arg1	tools					514:518	tools	514:518	tools	514:518	The grafted copolymer was characterized by FTIR, TGA and SEM tools.					
31442510	1	57	theme	grafted	228:234	arg1	copolymer					236:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer	153:244	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	1	57	theme	grafted	228:234	arg1	oil-adsorbent					275:287	a low-cost oil-adsorbent	264:287	a low-cost oil-adsorbent	264:287	A novel superoleophilic-hydrophobic nonanyl chitosan-poly (butyl acrylate) grafted copolymer was fabricated as a low-cost oil-adsorbent.					
31442510	8	58	theme	Thermodynamic	1264:1276	arg1	parameters					1278:1287	25 °C. Thermodynamic parameters	1257:1287	25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous	1257:1383	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	8	59	theme	adsorption	1076:1085	arg1	process					1087:1093	the adsorption process	1072:1093	the adsorption process	1072:1093	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	9	60	theme	potential	1442:1450	arg1	oil-					1452:1455	a potential oil-	1440:1455	a potential oil- adsorbent for petroleum oil spills removal	1440:1498	The results nominate the superoleophilic adsorbent as a potential oil- adsorbent for petroleum oil spills removal.					
31442510	8	61	theme	pseudo-second	1108:1120	arg1	model					1128:1132	the pseudo-second order model	1104:1132	the pseudo-second order model	1104:1132	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	0	62	theme	butyl	71:75	arg1	acrylate					77:84	butyl acrylate	71:84	butyl acrylate	71:84	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	0	62	theme	butyl	71:75	arg1	chitosan-poly					56:68	a novel low-cost superoleophilic nonanyl chitosan-poly	15:68	a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate)	15:85	Fabrication of a novel low-cost superoleophilic nonanyl chitosan-poly (butyl acrylate) grafted copolymer for the adsorptive removal of crude oil spills.					
31442510	8	63	theme	adsorption	1223:1232	arg1	108.79 g/g					1243:1252	a maximum adsorption capacity 108.79 g/g	1213:1252	a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous	1213:1383	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31442510	4	64	theme	ButA	590:593	arg1	concentration					595:607	ButA concentration	590:607	ButA concentration	590:607	The grafting percent was augmented and reached 88.5% with increasing ButA concentration up to 156 mM.					
31442510	2	65	theme	butyl	421:425	arg1	ButA					446:449	ButA	446:449	ButA	446:449	Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl acrylate monomers (ButA).					
31442510	2	65	theme	butyl	421:425	arg1	monomers					436:443	butyl acrylate monomers	421:443	butyl acrylate monomers (ButA)	421:450	Chitosan (CS) was coupled with a hydrophobic nonanal (N) to form nonanyl chitosan (NCS) schiff base, and followed by grafting with butyl acrylate monomers (ButA).					
31442510	5	66	theme	copolymer	721:729	arg1	character					690:698	the superoleophilic character	670:698	the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°	670:759	Moreover, measurements of contact angle proved the superoleophilic character of NCS-g-poly (ButA) copolymer with an oil-contact angle 31°.					
31442510	8	67	theme	maximum	1215:1221	arg1	108.79 g/g					1243:1252	a maximum adsorption capacity 108.79 g/g	1213:1252	a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous	1213:1383	Besides, the adsorption process followed the pseudo-second order model and the equilibrium data were sufficiently fitted with the Langmuir model with a maximum adsorption capacity 108.79 g/g at 25 °C. Thermodynamic parameters computed from Van't Hoff plot confirmed the process to be exothermic, favorable and spontaneous.					
31759027	7	0	theme	hyperglycemia	1807:1819	arg1	prevention					1780:1789	prevention	1780:1789	prevention	1780:1789	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	7	0	theme	hyperglycemia	1807:1819	arg1	treatment					1794:1802	treatment	1794:1802	treatment	1794:1802	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	4	1	theme	network	985:991	arg1	analysis					993:1000	Spearman's correlation network analysis	962:1000	Spearman's correlation network analysis	962:1000	Spearman's correlation network analysis revealed that key microbial phylotypes responding to GFP intervention were strongly correlated with the glucose and lipid metabolic disorders associated parameters.					
31759027	3	2	theme	intestinal	929:938	arg1	phylotypes					950:959	the functionally relevant intestinal microbial phylotypes	903:959	the functionally relevant intestinal microbial phylotypes	903:959	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	2	3	theme	TG	690:691	arg1	levels					676:681	the hepatic levels	664:681	the hepatic levels of TC, TG and free fatty acids (FFA)	664:718	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	4	theme	triglyceride	569:580	arg1	levels					482:487	the serum levels	472:487	the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C)	472:633	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	5	theme	TC	686:687	arg1	levels					676:681	the hepatic levels	664:681	the hepatic levels of TC, TG and free fatty acids (FFA)	664:718	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	1	6	from	frondosa	319:326	arg1	polysaccharides					290:304	polysaccharides	290:304	polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ)	290:405	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31759027	1	6	from	frondosa	319:326	arg1	activities					276:285	the hypoglycemic and hypolipidemic activities	241:285	the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ)	241:405	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31759027	7	7	theme	gut	1628:1630	arg1	microbiota					1632:1641	gut microbiota	1628:1641	gut microbiota	1628:1641	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	2	8	theme	tolerance	534:542	arg1	levels					482:487	the serum levels	472:487	the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C)	472:633	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	7	9	theme	food	1755:1758	arg1	ingredients					1760:1770	potential functional food ingredients	1734:1770	potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia	1734:1838	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	7	9	theme	food	1755:1758	arg1	GFP					1548:1550	GFP	1548:1550	GFP	1548:1550	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	2	10	theme	acids	708:712	arg1	levels					676:681	the hepatic levels	664:681	the hepatic levels of TC, TG and free fatty acids (FFA)	664:718	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	0	11	theme	microflora	136:145	arg1	modulation					111:120	the modulation	107:120	the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin	107:206	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	2	12	theme	oral	521:524	arg1	OGT					545:547	OGT	545:547	OGT	545:547	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	12	theme	oral	521:524	arg1	tolerance					534:542	oral glucose tolerance	521:542	oral glucose tolerance (OGT)	521:548	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	3	13	from	composition	828:838	arg1	mice					877:880	diabetic mice	868:880	diabetic mice	868:880	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	2	14	theme	free	697:700	arg1	FFA					715:717	FFA	715:717	FFA	715:717	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	14	theme	free	697:700	arg1	acids					708:712	free fatty acids	697:712	free fatty acids (FFA)	697:718	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	0	15	theme	diabetic	150:157	arg1	mice					159:162	diabetic mice	150:162	diabetic mice induced by high-fat diet and streptozotocin	150:206	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	7	16	used	used	1726:1729	arg2	GFP					1548:1550	GFP	1548:1550	GFP	1548:1550	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	7	16	used	used	1726:1729	arg2	ingredients					1760:1770	potential functional food ingredients	1734:1770	potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia	1734:1838	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	7	17	theme	diabetic	1602:1609	arg1	mice					1611:1614	diabetic mice	1602:1614	diabetic mice	1602:1614	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	5	18	theme	hepatic	1253:1259	arg1	glucose					1261:1267	hepatic glucose and lipid metabolism	1253:1288	glucose	1261:1267	Moreover, GFP treatment regulated mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism.					
31759027	0	19	theme	high-fat	175:182	arg1	diet					184:187	high-fat diet	175:187	high-fat diet	175:187	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	7	20	theme	hepatic	1658:1664	arg1	metabolism					1677:1686	hepatic glycolipid metabolism	1658:1686	hepatic glycolipid metabolism	1658:1686	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	2	21	theme	blood	500:504	arg1	FBG					515:517	FBG	515:517	FBG	515:517	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	21	theme	blood	500:504	arg1	glucose					506:512	fasting blood glucose	492:512	fasting blood glucose (FBG)	492:518	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	4	22	theme	glucose	1106:1112	arg1	disorders					1134:1142	the glucose and lipid metabolic disorders	1102:1142	the glucose and lipid metabolic disorders associated parameters	1102:1164	Spearman's correlation network analysis revealed that key microbial phylotypes responding to GFP intervention were strongly correlated with the glucose and lipid metabolic disorders associated parameters.					
31759027	5	23	theme	GFP	1177:1179	arg1	treatment					1181:1189	GFP treatment	1177:1189	GFP treatment	1177:1189	Moreover, GFP treatment regulated mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism.					
31759027	0	24	theme	hypolipidemic	17:29	arg1	activities					31:40	Hypoglycemic and hypolipidemic activities	0:40	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.	0:207	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	4	25	theme	lipid	1118:1122	arg1	disorders					1134:1142	the glucose and lipid metabolic disorders	1102:1142	the glucose and lipid metabolic disorders associated parameters	1102:1164	Spearman's correlation network analysis revealed that key microbial phylotypes responding to GFP intervention were strongly correlated with the glucose and lipid metabolic disorders associated parameters.					
31759027	3	26	theme	of	742:743	arg1	supplementation					749:763	high-dose of GFP supplementation	732:763	high-dose of GFP supplementation (900 mg/kg day)	732:779	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	3	26	theme	of	742:743	arg1	day					776:778	900 mg/kg day	766:778	900 mg/kg day	766:778	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	3	26	theme	of	742:743	arg1	Meanwhile					721:729	Meanwhile	721:729	Meanwhile	721:729	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	0	27	theme	relationships	88:100	arg1	activities					31:40	Hypoglycemic and hypolipidemic activities	0:40	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.	0:207	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	1	28	theme	high-fat	362:369	arg1	HFD					377:379	HFD	377:379	HFD	377:379	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31759027	1	28	theme	high-fat	362:369	arg1	diet					371:374	high-fat diet	362:374	high-fat diet (HFD)	362:380	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31759027	7	29	from	hyperlipidemia	1584:1597	arg1	mice					1611:1614	diabetic mice	1602:1614	diabetic mice	1602:1614	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	2	30	theme	low-density	591:601	arg1	LDL-C					628:632	LDL-C	628:632	LDL-C	628:632	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	30	theme	low-density	591:601	arg1	cholesterol					615:625	low-density lipoprotein cholesterol	591:625	low-density lipoprotein cholesterol (LDL-C)	591:633	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	5	31	theme	responsible	1237:1247	arg1	genes					1231:1235	the genes	1227:1235	the genes responsible for hepatic glucose and lipid metabolism	1227:1288	Moreover, GFP treatment regulated mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism.					
31759027	6	32	from	excretion	1495:1503	arg1	liver					1508:1512	liver	1508:1512	liver	1508:1512	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	7	33	theme	related	1688:1694	arg1	genes					1696:1700	related genes	1688:1700	related genes	1688:1700	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	6	34	theme	mRNA	1346:1349	arg1	expression					1351:1360	mRNA expression	1346:1360	mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP)	1346:1432	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	0	35	theme	Grifola	45:51	arg1	polysaccharides					62:76	Grifola frondosa polysaccharides	45:76	Grifola frondosa polysaccharides	45:76	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	7	36	theme	glycolipid	1666:1675	arg1	metabolism					1677:1686	hepatic glycolipid metabolism	1658:1686	hepatic glycolipid metabolism	1658:1686	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	2	37	theme	GFP	451:453	arg1	administration					433:446	oral administration	428:446	oral administration of GFP	428:453	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	0	38	theme	Hypoglycemic	0:11	arg1	activities					31:40	Hypoglycemic and hypolipidemic activities	0:40	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.	0:207	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	3	39	theme	intestinal	843:852	arg1	microflora					854:863	intestinal microflora	843:863	intestinal microflora	843:863	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	4	40	theme	correlation	973:983	arg1	analysis					993:1000	Spearman's correlation network analysis	962:1000	Spearman's correlation network analysis	962:1000	Spearman's correlation network analysis revealed that key microbial phylotypes responding to GFP intervention were strongly correlated with the glucose and lipid metabolic disorders associated parameters.					
31759027	2	41	theme	hepatic	668:674	arg1	levels					676:681	the hepatic levels	664:681	the hepatic levels of TC, TG and free fatty acids (FFA)	664:718	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	42	theme	cholesterol	615:625	arg1	levels					482:487	the serum levels	472:487	the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C)	472:633	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	6	43	theme	pump	1422:1425	arg1	expression					1351:1360	mRNA expression	1346:1360	mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP)	1346:1432	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	2	44	theme	serum	476:480	arg1	levels					482:487	the serum levels	472:487	the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C)	472:633	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	6	45	theme	salt	1410:1413	arg1	BSEP					1428:1431	BSEP	1428:1431	BSEP	1428:1431	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	6	45	theme	salt	1410:1413	arg1	pump					1422:1425	bile salt export pump	1405:1425	bile salt export pump (BSEP)	1405:1432	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	0	46	theme	frondosa	53:60	arg1	polysaccharides					62:76	Grifola frondosa polysaccharides	45:76	Grifola frondosa polysaccharides	45:76	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	1	47	theme	diabetic	337:344	arg1	mice					346:349	diabetic mice	337:349	diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ)	337:405	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31759027	4	48	theme	microbial	1020:1028	arg1	phylotypes					1030:1039	key microbial phylotypes	1016:1039	key microbial phylotypes responding to GFP intervention	1016:1070	Spearman's correlation network analysis revealed that key microbial phylotypes responding to GFP intervention were strongly correlated with the glucose and lipid metabolic disorders associated parameters.					
31759027	6	49	theme	acids	1469:1473	arg1	synthesis					1481:1489	bile acids (BAs) synthesis	1464:1489	bile acids (BAs) synthesis	1464:1489	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	7	50	from	hyperglycemia	1566:1578	arg1	mice					1611:1614	diabetic mice	1602:1614	diabetic mice	1602:1614	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	5	51	theme	expression	1206:1215	arg1	levels					1217:1222	mRNA expression levels	1201:1222	mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism	1201:1288	Moreover, GFP treatment regulated mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism.					
31759027	0	52	theme	polysaccharides	62:76	arg1	activities					31:40	Hypoglycemic and hypolipidemic activities	0:40	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.	0:207	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	0	53	from	microflora	136:145	arg1	mice					159:162	diabetic mice	150:162	diabetic mice induced by high-fat diet and streptozotocin	150:206	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	3	54	theme	microbial	940:948	arg1	phylotypes					950:959	the functionally relevant intestinal microbial phylotypes	903:959	the functionally relevant intestinal microbial phylotypes	903:959	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	0	55	with	relationships	88:100	arg1	modulation					111:120	the modulation	107:120	the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin	107:206	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	1	56	theme	polysaccharides	290:304	arg1	activities					276:285	the hypoglycemic and hypolipidemic activities	241:285	the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ)	241:405	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31759027	2	57	theme	cholesterol	551:561	arg1	levels					482:487	the serum levels	472:487	the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C)	472:633	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	3	58	theme	relevant	920:927	arg1	phylotypes					950:959	the functionally relevant intestinal microbial phylotypes	903:959	the functionally relevant intestinal microbial phylotypes	903:959	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	6	59	theme	7α-hydroxylase	1377:1390	arg1	expression					1351:1360	mRNA expression	1346:1360	mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP)	1346:1432	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	7	60	theme	functional	1744:1753	arg1	ingredients					1760:1770	potential functional food ingredients	1734:1770	potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia	1734:1838	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	7	60	theme	functional	1744:1753	arg1	GFP					1548:1550	GFP	1548:1550	GFP	1548:1550	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	4	61	theme	GFP	1055:1057	arg1	intervention					1059:1070	GFP intervention	1055:1070	GFP intervention	1055:1070	Spearman's correlation network analysis revealed that key microbial phylotypes responding to GFP intervention were strongly correlated with the glucose and lipid metabolic disorders associated parameters.					
31759027	6	62	theme	synthesis	1481:1489	arg1	enhancement					1449:1459	an enhancement	1446:1459	an enhancement of bile acids (BAs) synthesis	1446:1489	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	6	62	theme	synthesis	1481:1489	arg1	excretion					1495:1503	excretion	1495:1503	excretion in liver	1495:1512	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	2	63	theme	glucose	526:532	arg1	OGT					545:547	OGT	545:547	OGT	545:547	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	63	theme	glucose	526:532	arg1	tolerance					534:542	oral glucose tolerance	521:542	oral glucose tolerance (OGT)	521:548	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	64	theme	fatty	702:706	arg1	FFA					715:717	FFA	715:717	FFA	715:717	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	64	theme	fatty	702:706	arg1	acids					708:712	free fatty acids	697:712	free fatty acids (FFA)	697:718	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	1	65	from	activities	276:285	arg1	GFP					329:331	GFP	329:331	GFP	329:331	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31759027	1	65	from	activities	276:285	arg1	frondosa					319:326	Grifola frondosa	311:326	Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ)	311:405	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31759027	6	66	theme	It is noteworthy that	1291:1311	arg1	treatment					1317:1325	It is noteworthy that GFP treatment	1291:1325	It is noteworthy that GFP treatment	1291:1325	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	7	67	theme	potential	1734:1742	arg1	ingredients					1760:1770	potential functional food ingredients	1734:1770	potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia	1734:1838	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	7	67	theme	potential	1734:1742	arg1	GFP					1548:1550	GFP	1548:1550	GFP	1548:1550	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	0	68	from	modulation	111:120	arg1	mice					159:162	diabetic mice	150:162	diabetic mice induced by high-fat diet and streptozotocin	150:206	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	7	69	dep	prevention	1780:1789	arg1	the					1776:1778	the	1776:1778	the	1776:1778	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	2	70	theme	fasting	492:498	arg1	FBG					515:517	FBG	515:517	FBG	515:517	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	70	theme	fasting	492:498	arg1	glucose					506:512	fasting blood glucose	492:512	fasting blood glucose (FBG)	492:518	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	4	71	theme	metabolic	1124:1132	arg1	disorders					1134:1142	the glucose and lipid metabolic disorders	1102:1142	the glucose and lipid metabolic disorders associated parameters	1102:1164	Spearman's correlation network analysis revealed that key microbial phylotypes responding to GFP intervention were strongly correlated with the glucose and lipid metabolic disorders associated parameters.					
31759027	3	72	theme	powerful	793:800	arg1	effects					802:808	powerful effects	793:808	powerful effects on moderating the composition of intestinal microflora in diabetic mice	793:880	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	2	73	theme	lipoprotein	603:613	arg1	LDL-C					628:632	LDL-C	628:632	LDL-C	628:632	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	2	73	theme	lipoprotein	603:613	arg1	cholesterol					615:625	low-density lipoprotein cholesterol	591:625	low-density lipoprotein cholesterol (LDL-C)	591:633	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	5	74	theme	genes	1231:1235	arg1	levels					1217:1222	mRNA expression levels	1201:1222	mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism	1201:1288	Moreover, GFP treatment regulated mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism.					
31759027	3	75	theme	GFP	745:747	arg1	supplementation					749:763	high-dose of GFP supplementation	732:763	high-dose of GFP supplementation (900 mg/kg day)	732:779	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	3	75	theme	GFP	745:747	arg1	day					776:778	900 mg/kg day	766:778	900 mg/kg day	766:778	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	3	75	theme	GFP	745:747	arg1	Meanwhile					721:729	Meanwhile	721:729	Meanwhile	721:729	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	6	76	theme	cholesterol	1365:1375	arg1	CYP7A1					1393:1398	CYP7A1	1393:1398	CYP7A1	1393:1398	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	6	76	theme	cholesterol	1365:1375	arg1	7α-hydroxylase					1377:1390	cholesterol 7α-hydroxylase	1365:1390	cholesterol 7α-hydroxylase (CYP7A1)	1365:1399	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	3	77	theme	high-dose	732:740	arg1	supplementation					749:763	high-dose of GFP supplementation	732:763	high-dose of GFP supplementation (900 mg/kg day)	732:779	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	3	77	theme	high-dose	732:740	arg1	day					776:778	900 mg/kg day	766:778	900 mg/kg day	766:778	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	3	77	theme	high-dose	732:740	arg1	Meanwhile					721:729	Meanwhile	721:729	Meanwhile	721:729	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	0	78	theme	intestinal	125:134	arg1	microflora					136:145	intestinal microflora	125:145	intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin	125:206	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	2	79	theme	glucose	506:512	arg1	levels					482:487	the serum levels	472:487	the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C)	472:633	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	1	80	theme	hypoglycemic	245:256	arg1	activities					276:285	the hypoglycemic and hypolipidemic activities	241:285	the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ)	241:405	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31759027	5	81	theme	lipid	1273:1277	arg1	metabolism					1279:1288	hepatic glucose and lipid metabolism	1253:1288	metabolism	1279:1288	Moreover, GFP treatment regulated mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism.					
31759027	3	82	theme	microflora	854:863	arg1	composition					828:838	the composition	824:838	the composition of intestinal microflora in diabetic mice	824:880	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	2	83	theme	oral	428:431	arg1	administration					433:446	oral administration	428:446	oral administration of GFP	428:453	Results showed that oral administration of GFP markedly reduced the serum levels of fasting blood glucose (FBG), oral glucose tolerance (OGT), cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly decreased the hepatic levels of TC, TG and free fatty acids (FFA).					
31759027	6	84	theme	export	1415:1420	arg1	BSEP					1428:1431	BSEP	1428:1431	BSEP	1428:1431	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	6	84	theme	export	1415:1420	arg1	pump					1422:1425	bile salt export pump	1405:1425	bile salt export pump (BSEP)	1405:1432	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	6	85	theme	bile	1405:1408	arg1	BSEP					1428:1431	BSEP	1428:1431	BSEP	1428:1431	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	6	85	theme	bile	1405:1408	arg1	pump					1422:1425	bile salt export pump	1405:1425	bile salt export pump (BSEP)	1405:1432	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	6	86	theme	GFP	1313:1315	arg1	treatment					1317:1325	It is noteworthy that GFP treatment	1291:1325	It is noteworthy that GFP treatment	1291:1325	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	3	87	theme	diabetic	868:875	arg1	mice					877:880	diabetic mice	868:880	diabetic mice	868:880	Meanwhile, high-dose of GFP supplementation (900 mg/kg day) also showed powerful effects on moderating the composition of intestinal microflora in diabetic mice, especially altering the functionally relevant intestinal microbial phylotypes.					
31759027	4	88	theme	key	1016:1018	arg1	phylotypes					1030:1039	key microbial phylotypes	1016:1039	key microbial phylotypes responding to GFP intervention	1016:1070	Spearman's correlation network analysis revealed that key microbial phylotypes responding to GFP intervention were strongly correlated with the glucose and lipid metabolic disorders associated parameters.					
31759027	0	89	from	mice	159:162	arg1	modulation					111:120	the modulation	107:120	the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin	107:206	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	0	90	with	polysaccharides	62:76	arg1	modulation					111:120	the modulation	107:120	the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin	107:206	Hypoglycemic and hypolipidemic activities of Grifola frondosa polysaccharides and their relationships with the modulation of intestinal microflora in diabetic mice induced by high-fat diet and streptozotocin.					
31759027	6	91	theme	bile	1464:1467	arg1	BAs					1476:1478	BAs	1476:1478	BAs	1476:1478	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	6	91	theme	bile	1464:1467	arg1	acids					1469:1473	bile acids	1464:1473	bile acids (BAs) synthesis	1464:1489	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	5	92	theme	mRNA	1201:1204	arg1	levels					1217:1222	mRNA expression levels	1201:1222	mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism	1201:1288	Moreover, GFP treatment regulated mRNA expression levels of the genes responsible for hepatic glucose and lipid metabolism.					
31759027	6	93	from	enhancement	1449:1459	arg1	liver					1508:1512	liver	1508:1512	liver	1508:1512	It is noteworthy that GFP treatment markedly increased mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) and bile salt export pump (BSEP), suggesting an enhancement of bile acids (BAs) synthesis and excretion in liver.					
31759027	7	94	theme	hyperlipidemia	1825:1838	arg1	prevention					1780:1789	prevention	1780:1789	prevention	1780:1789	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	7	94	theme	hyperlipidemia	1825:1838	arg1	treatment					1794:1802	treatment	1794:1802	treatment	1794:1802	These findings demonstrated that GFP could prevent hyperglycemia and hyperlipidemia in diabetic mice by altering gut microbiota and regulating hepatic glycolipid metabolism related genes, and therefore could be used as potential functional food ingredients for the prevention or treatment of hyperglycemia and hyperlipidemia.					
31759027	1	95	theme	hypolipidemic	262:274	arg1	activities					276:285	the hypoglycemic and hypolipidemic activities	241:285	the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ)	241:405	This study aimed to investigate the hypoglycemic and hypolipidemic activities of polysaccharides from Grifola frondosa (GFP) in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ).					
31626205	7	0	theme	epithelial	2015:2024	arg1	cells					2026:2030	the epithelial cells	2011:2030	the epithelial cells in experimental acute and chronic ulcerative colitis	2011:2083	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	3	1	theme	subpopulation	693:705	arg1	composition					707:717	the subpopulation composition	689:717	the subpopulation composition of lymphocytes and thymic stromal cells	689:757	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	7	2	theme	accidental	1864:1873	arg1	involution					1875:1884	acute accidental involution	1858:1884	acute accidental involution	1858:1884	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	1	3	theme	non-lymphoid	250:261	arg1	cells					263:267	its non-lymphoid cells	246:267	its non-lymphoid cells	246:267	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	4	4	theme	catarrhal	902:910	arg1	colitis					927:933	acute catarrhal and ulcerative colitis	896:933	acute catarrhal and ulcerative colitis	896:933	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	8	5	theme	experimental	2164:2175	arg1	colitis					2196:2202	end-stage experimental chronic ulcerative colitis	2154:2202	end-stage experimental chronic ulcerative colitis	2154:2202	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	3	6	theme	stromal	745:751	arg1	cells					753:757	thymic stromal cells	738:757	thymic stromal cells	738:757	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	7	7	dep	CONCLUSION	1838:1847	arg1	is					1855:1856	is	1855:1856	is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively	1855:2097	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	8	8	theme	ulcerative	2185:2194	arg1	colitis					2196:2202	end-stage experimental chronic ulcerative colitis	2154:2202	end-stage experimental chronic ulcerative colitis	2154:2202	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	7	9	theme	epithelial	1939:1948	arg1	cells					1950:1954	medullary epithelial cells	1929:1954	medullary epithelial cells	1929:1954	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	3	10	dep	number	601:606	arg1	the					597:599	the	597:599	the	597:599	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	4	11	theme	apoptosis	1138:1146	arg1	mechanism					1125:1133	the mechanism	1121:1133	the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them	1121:1416	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	12	theme	corpuscles	1352:1361	arg1	area					1337:1340	the area	1333:1340	the area of thymic corpuscles	1333:1361	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	13	from	mechanism	1125:1133	arg1	content					1374:1380	the content	1370:1380	the content of late-phase corpuscles among them	1370:1416	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	7	14	theme	thymic	1960:1965	arg1	corpuscles					1967:1976	thymic corpuscles	1960:1976	thymic corpuscles	1960:1976	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	8	15	theme	ulcerative	2383:2392	arg1	colitis					2394:2400	ulcerative colitis	2383:2400	ulcerative colitis	2383:2400	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	8	16	theme	enhanced	2217:2224	arg1	differentiation					2226:2240	the enhanced differentiation	2213:2240	the enhanced differentiation of regulatory T-lymphocytes	2213:2268	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	4	17	theme	cells	1101:1105	arg1	levels					1091:1096	the levels	1087:1096	the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them	1087:1416	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	5	18	theme	epithelial	1603:1612	arg1	cells					1614:1618	medullary epithelial cells	1593:1618	medullary epithelial cells	1593:1618	In chronic ulcerative colitis, the cortex was expanded and the area of thymic corpuscles and the count of medullary epithelial cells increased.					
31626205	0	19	theme	chronic	106:112	arg1	colitis					125:131	experimental acute and chronic ulcerative colitis	83:131	experimental acute and chronic ulcerative colitis	83:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	8	20	with	patients	2369:2376	arg1	colitis					2394:2400	ulcerative colitis	2383:2400	ulcerative colitis	2383:2400	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	4	21	theme	accidental	952:961	arg1	involution					970:979	acute accidental thymic involution	946:979	acute accidental thymic involution	946:979	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	6	22	theme	development	1825:1835	arg1	periods					1810:1816	both periods	1805:1816	both periods of its development	1805:1835	The cyst-like thymic corpuscles formed clusters, the count of dendritic cells increased in early-stage chronic ulcerative colitis, but the levels of macrophages decreased in both periods of its development.					
31626205	5	23	theme	corpuscles	1565:1574	arg1	area					1550:1553	the area	1546:1553	the area of thymic corpuscles	1546:1574	In chronic ulcerative colitis, the cortex was expanded and the area of thymic corpuscles and the count of medullary epithelial cells increased.					
31626205	5	23	theme	corpuscles	1565:1574	arg1	count					1584:1588	the count	1580:1588	the count of medullary epithelial cells	1580:1618	In chronic ulcerative colitis, the cortex was expanded and the area of thymic corpuscles and the count of medullary epithelial cells increased.					
31626205	3	24	theme	absolute	612:619	arg1	area					621:624	absolute area	612:624	absolute area	612:624	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	1	25	theme	subpopulation	201:213	arg1	composition					215:225	the subpopulation composition	197:225	the subpopulation composition of lymphocytes	197:240	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	7	26	theme	medullary	1929:1937	arg1	cells					1950:1954	medullary epithelial cells	1929:1954	medullary epithelial cells	1929:1954	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	4	27	theme	ulcerative	916:925	arg1	colitis					927:933	acute catarrhal and ulcerative colitis	896:933	acute catarrhal and ulcerative colitis	896:933	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	6	28	theme	cyst-like	1635:1643	arg1	corpuscles					1652:1661	The cyst-like thymic corpuscles	1631:1661	The cyst-like thymic corpuscles	1631:1661	The cyst-like thymic corpuscles formed clusters, the count of dendritic cells increased in early-stage chronic ulcerative colitis, but the levels of macrophages decreased in both periods of its development.					
31626205	8	29	theme	T-lymphocytes	2256:2268	arg1	differentiation					2226:2240	the enhanced differentiation	2213:2240	the enhanced differentiation of regulatory T-lymphocytes	2213:2268	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	8	29	theme	T-lymphocytes	2256:2268	arg1	number					2285:2290	the larger number	2274:2290	the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature	2274:2429	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	4	30	theme	absolute	1176:1183	arg1	number					1185:1190	the absolute number	1172:1190	the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells	1172:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	3	31	theme	corpuscles	636:645	arg1	number					601:606	number	601:606	number	601:606	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	3	31	theme	corpuscles	636:645	arg1	area					621:624	absolute area	612:624	absolute area	612:624	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	2	32	theme	%	507:507	arg1	solution					540:547	a 1% aqueous dextran sulfate sodium solution	504:547	a 1% aqueous dextran sulfate sodium solution	504:547	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	7	33	theme	chronic	2058:2064	arg1	colitis					2077:2083	experimental acute and chronic ulcerative colitis	2035:2083	experimental acute and chronic ulcerative colitis	2035:2083	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	4	34	theme	substance	1014:1022	arg1	devastation					986:996	devastation	986:996	devastation of the cortical substance	986:1022	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	6	35	theme	cells	1703:1707	arg1	count					1684:1688	the count	1680:1688	the count of dendritic cells	1680:1707	The cyst-like thymic corpuscles formed clusters, the count of dendritic cells increased in early-stage chronic ulcerative colitis, but the levels of macrophages decreased in both periods of its development.					
31626205	7	36	theme	acute	2048:2052	arg1	colitis					2077:2083	experimental acute and chronic ulcerative colitis	2035:2083	experimental acute and chronic ulcerative colitis	2035:2083	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	0	37	theme	cellular	59:66	arg1	composition					68:78	its cellular composition	55:78	its cellular composition in experimental acute and chronic ulcerative colitis	55:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	1	38	theme	chronic	354:360	arg1	colitis					373:379	chronic ulcerative colitis	354:379	chronic ulcerative colitis	354:379	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	2	39	theme	sodium	533:538	arg1	solution					540:547	a 1% aqueous dextran sulfate sodium solution	504:547	a 1% aqueous dextran sulfate sodium solution	504:547	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	2	40	from	mice	465:468	arg1	simulated					444:452	simulated	444:452	simulated	444:452	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	4	41	theme	cells	1217:1221	arg1	number					1185:1190	the absolute number	1172:1190	the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells	1172:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	42	theme	late-phase	1385:1394	arg1	corpuscles					1396:1405	late-phase corpuscles	1385:1405	late-phase corpuscles among them	1385:1416	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	1	43	theme	colitis	373:379	arg1	periods					343:349	different periods	333:349	different periods of chronic ulcerative colitis	333:379	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	2	44	theme	dextran	517:523	arg1	solution					540:547	a 1% aqueous dextran sulfate sodium solution	504:547	a 1% aqueous dextran sulfate sodium solution	504:547	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	4	45	theme	cytotoxic	1224:1232	arg1	T-cells					1234:1240	cytotoxic T-cells	1224:1240	cytotoxic T-cells	1224:1240	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	2	46	theme	chronic	413:419	arg1	colitis					432:438	Acute and chronic ulcerative colitis	403:438	Acute and chronic ulcerative colitis	403:438	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	0	47	theme	acute	96:100	arg1	colitis					125:131	experimental acute and chronic ulcerative colitis	83:131	experimental acute and chronic ulcerative colitis	83:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	4	48	theme	corpuscles	1396:1405	arg1	content					1374:1380	the content	1370:1380	the content of late-phase corpuscles among them	1370:1416	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	3	49	used	used	857:860	arg2	test					821:824	the Kruskal-Wallis test	802:824	the Kruskal-Wallis test	802:824	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	3	49	used	used	857:860	arg2	test					847:850	the Mann-Whitney test	830:850	the Mann-Whitney test	830:850	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	3	50	theme	cells	753:757	arg1	composition					707:717	the subpopulation composition	689:717	the subpopulation composition of lymphocytes and thymic stromal cells	689:757	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	2	51	theme	Acute	403:407	arg1	colitis					432:438	Acute and chronic ulcerative colitis	403:438	Acute and chronic ulcerative colitis	403:438	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	1	52	theme	dextran-induced	272:286	arg1	colitis					318:324	dextran-induced experimental acute ulcerative colitis	272:324	dextran-induced experimental acute ulcerative colitis	272:324	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	2	53	theme	C57BL/6	457:463	arg1	mice					465:468	C57BL/6 mice	457:468	C57BL/6 mice	457:468	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	5	54	theme	chronic	1490:1496	arg1	colitis					1509:1515	chronic ulcerative colitis	1490:1515	chronic ulcerative colitis	1490:1515	In chronic ulcerative colitis, the cortex was expanded and the area of thymic corpuscles and the count of medullary epithelial cells increased.					
31626205	5	55	theme	cells	1614:1618	arg1	area					1550:1553	the area	1546:1553	the area of thymic corpuscles	1546:1574	In chronic ulcerative colitis, the cortex was expanded and the area of thymic corpuscles and the count of medullary epithelial cells increased.					
31626205	5	55	theme	cells	1614:1618	arg1	count					1584:1588	the count	1580:1588	the count of medullary epithelial cells	1580:1618	In chronic ulcerative colitis, the cortex was expanded and the area of thymic corpuscles and the count of medullary epithelial cells increased.					
31626205	1	56	theme	acute	301:305	arg1	colitis					318:324	dextran-induced experimental acute ulcerative colitis	272:324	dextran-induced experimental acute ulcerative colitis	272:324	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	4	57	with	apoptosis	1138:1146	arg1	decrease					1160:1167	a decrease	1158:1167	a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells	1158:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	57	with	apoptosis	1138:1146	arg1	rise					1312:1315	a rise	1310:1315	a rise in the index of the area of thymic corpuscles	1310:1361	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	7	58	from	cytokeratin	1992:2002	arg1	cells					2026:2030	the epithelial cells	2011:2030	the epithelial cells in experimental acute and chronic ulcerative colitis	2011:2083	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	4	59	theme	B-lymphocytes	1269:1281	arg1	number					1185:1190	the absolute number	1172:1190	the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells	1172:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	60	from	decline	1035:1041	arg1	fraction					1057:1064	its volume fraction	1046:1064	its volume fraction	1046:1064	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	6	61	theme	chronic	1734:1740	arg1	colitis					1753:1759	early-stage chronic ulcerative colitis	1722:1759	early-stage chronic ulcerative colitis	1722:1759	The cyst-like thymic corpuscles formed clusters, the count of dendritic cells increased in early-stage chronic ulcerative colitis, but the levels of macrophages decreased in both periods of its development.					
31626205	3	62	theme	epithelial	651:660	arg1	cells					662:666	epithelial cells	651:666	epithelial cells	651:666	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	0	63	theme	specific	34:41	arg1	features					43:50	the specific features	30:50	the specific features of its cellular composition in experimental acute and chronic ulcerative colitis	30:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	8	64	theme	peripheral	2316:2325	arg1	blood					2327:2331	peripheral blood	2316:2331	peripheral blood	2316:2331	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	8	65	theme	pronounced	2109:2118	arg1	response					2136:2143	The more pronounced epithelial cell response	2100:2143	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis	2100:2202	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	0	66	from	colitis	125:131	arg1	[Morphology					0:10	[Morphology	0:10	[Morphology of the thymus	0:24	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	0	66	from	colitis	125:131	arg1	features					43:50	the specific features	30:50	the specific features of its cellular composition in experimental acute and chronic ulcerative colitis	30:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	4	67	theme	T-lymphocytes	1254:1266	arg1	number					1185:1190	the absolute number	1172:1190	the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells	1172:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	68	from	apoptosis	1138:1146	arg1	content					1374:1380	the content	1370:1380	the content of late-phase corpuscles among them	1370:1416	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	1	69	theme	lymphocytes	230:240	arg1	composition					215:225	the subpopulation composition	197:225	the subpopulation composition of lymphocytes	197:240	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	1	69	theme	lymphocytes	230:240	arg1	cells					263:267	its non-lymphoid cells	246:267	its non-lymphoid cells	246:267	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	1	69	theme	lymphocytes	230:240	arg1	thymus					189:194	the thymus	185:194	the thymus	185:194	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	7	70	theme	experimental	2035:2046	arg1	colitis					2077:2083	experimental acute and chronic ulcerative colitis	2035:2083	experimental acute and chronic ulcerative colitis	2035:2083	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	2	71	from	simulated	444:452	arg1	mice					465:468	C57BL/6 mice	457:468	C57BL/6 mice	457:468	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	8	72	theme	cell	2131:2134	arg1	response					2136:2143	The more pronounced epithelial cell response	2100:2143	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis	2100:2202	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	3	73	theme	lymphocytes	722:732	arg1	composition					707:717	the subpopulation composition	689:717	the subpopulation composition of lymphocytes and thymic stromal cells	689:757	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	6	74	theme	macrophages	1780:1790	arg1	levels					1770:1775	the levels	1766:1775	the levels of macrophages	1766:1790	The cyst-like thymic corpuscles formed clusters, the count of dendritic cells increased in early-stage chronic ulcerative colitis, but the levels of macrophages decreased in both periods of its development.					
31626205	3	75	theme	Thymic	550:555	arg1	changes					557:563	Thymic changes	550:563	Thymic changes	550:563	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	4	76	theme	acute	896:900	arg1	colitis					927:933	acute catarrhal and ulcerative colitis	896:933	acute catarrhal and ulcerative colitis	896:933	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	8	77	theme	end-stage	2154:2162	arg1	colitis					2196:2202	end-stage experimental chronic ulcerative colitis	2154:2202	end-stage experimental chronic ulcerative colitis	2154:2202	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	4	78	from	content	1374:1380	arg1	mechanism					1125:1133	the mechanism	1121:1133	the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them	1121:1416	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	79	theme	cells	1298:1302	arg1	number					1185:1190	the absolute number	1172:1190	the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells	1172:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	1	80	theme	morphological	160:172	arg1	changes					174:180	morphological changes	160:180	morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis	160:379	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	4	81	dep	RESULTS	885:891	arg1	was					942:944	was	942:944	was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities	942:1484	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	8	82	theme	chronic	2177:2183	arg1	colitis					2196:2202	end-stage experimental chronic ulcerative colitis	2154:2202	end-stage experimental chronic ulcerative colitis	2154:2202	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	3	83	theme	thymic	738:743	arg1	cells					753:757	thymic stromal cells	738:757	thymic stromal cells	738:757	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	8	84	from	focus	2344:2348	arg1	patients					2369:2376	patients	2369:2376	patients with ulcerative colitis	2369:2400	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	5	85	theme	medullary	1593:1601	arg1	cells					1614:1618	medullary epithelial cells	1593:1618	medullary epithelial cells	1593:1618	In chronic ulcerative colitis, the cortex was expanded and the area of thymic corpuscles and the count of medullary epithelial cells increased.					
31626205	4	86	theme	thymic	1345:1350	arg1	corpuscles					1352:1361	thymic corpuscles	1345:1361	thymic corpuscles	1345:1361	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	0	87	theme	ulcerative	114:123	arg1	colitis					125:131	experimental acute and chronic ulcerative colitis	83:131	experimental acute and chronic ulcerative colitis	83:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	0	88	from	composition	68:78	arg1	colitis					125:131	experimental acute and chronic ulcerative colitis	83:131	experimental acute and chronic ulcerative colitis	83:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	5	89	theme	thymic	1558:1563	arg1	corpuscles					1565:1574	thymic corpuscles	1558:1574	thymic corpuscles	1558:1574	In chronic ulcerative colitis, the cortex was expanded and the area of thymic corpuscles and the count of medullary epithelial cells increased.					
31626205	8	90	from	patients	2369:2376	arg1	focus					2344:2348	the focus	2340:2348	the focus of inflammation in patients with ulcerative colitis	2340:2400	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	4	91	theme	acute	946:950	arg1	involution					970:979	acute accidental thymic involution	946:979	acute accidental thymic involution	946:979	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	6	92	theme	thymic	1645:1650	arg1	corpuscles					1652:1661	The cyst-like thymic corpuscles	1631:1661	The cyst-like thymic corpuscles	1631:1661	The cyst-like thymic corpuscles formed clusters, the count of dendritic cells increased in early-stage chronic ulcerative colitis, but the levels of macrophages decreased in both periods of its development.					
31626205	3	93	theme	Kruskal-Wallis	806:819	arg1	test					821:824	the Kruskal-Wallis test	802:824	the Kruskal-Wallis test	802:824	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	3	94	theme	flow	780:783	arg1	cytofluorimetry					785:799	flow cytofluorimetry	780:799	flow cytofluorimetry	780:799	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	8	95	from	inflammation	2353:2364	arg1	patients					2369:2376	patients	2369:2376	patients with ulcerative colitis	2369:2400	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	0	96	from	[Morphology	0:10	arg1	colitis					125:131	experimental acute and chronic ulcerative colitis	83:131	experimental acute and chronic ulcerative colitis	83:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	7	97	from	increase	1917:1924	arg1	cells					1950:1954	medullary epithelial cells	1929:1954	medullary epithelial cells	1929:1954	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	7	97	from	increase	1917:1924	arg1	corpuscles					1967:1976	thymic corpuscles	1960:1976	thymic corpuscles	1960:1976	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	8	98	theme	regulatory	2245:2254	arg1	T-lymphocytes					2256:2268	regulatory T-lymphocytes	2245:2268	regulatory T-lymphocytes	2245:2268	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	4	99	theme	area	1337:1340	arg1	index					1324:1328	the index	1320:1328	the index of the area of thymic corpuscles	1320:1361	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	8	100	located	found	2145:2149	arg2	response					2136:2143	The more pronounced epithelial cell response	2100:2143	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis	2100:2202	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	8	100	located	found	2145:2149	arg1	colitis					2196:2202	end-stage experimental chronic ulcerative colitis	2154:2202	end-stage experimental chronic ulcerative colitis	2154:2202	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	3	101	theme	thymic	629:634	arg1	corpuscles					636:645	thymic corpuscles	629:645	thymic corpuscles	629:645	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	3	102	theme	Mann-Whitney	834:845	arg1	test					847:850	the Mann-Whitney test	830:850	the Mann-Whitney test	830:850	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	4	103	from	increase	1075:1082	arg1	levels					1091:1096	the levels	1087:1096	the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them	1087:1416	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	104	theme	thymic	1446:1451	arg1	corpuscles					1453:1462	thymic corpuscles	1446:1462	thymic corpuscles as cyst-like cavities	1446:1484	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	2	105	theme	1	506:506	arg1	%					507:507	%	507:507	%	507:507	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	8	106	theme	larger	2278:2283	arg1	number					2285:2290	the larger number	2274:2290	the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature	2274:2429	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	7	107	theme	ulcerative	2066:2075	arg1	colitis					2077:2083	experimental acute and chronic ulcerative colitis	2035:2083	experimental acute and chronic ulcerative colitis	2035:2083	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	4	108	theme	thymic	963:968	arg1	involution					970:979	acute accidental thymic involution	946:979	acute accidental thymic involution	946:979	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	0	109	theme	composition	68:78	arg1	[Morphology					0:10	[Morphology	0:10	[Morphology of the thymus	0:24	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	0	109	theme	composition	68:78	arg1	features					43:50	the specific features	30:50	the specific features of its cellular composition in experimental acute and chronic ulcerative colitis	30:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	1	110	from	changes	174:180	arg1	periods					343:349	different periods	333:349	different periods of chronic ulcerative colitis	333:379	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	1	110	from	changes	174:180	arg1	composition					215:225	the subpopulation composition	197:225	the subpopulation composition of lymphocytes	197:240	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	1	110	from	changes	174:180	arg1	cells					263:267	its non-lymphoid cells	246:267	its non-lymphoid cells	246:267	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	1	110	from	changes	174:180	arg1	colitis					318:324	dextran-induced experimental acute ulcerative colitis	272:324	dextran-induced experimental acute ulcerative colitis	272:324	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	1	110	from	changes	174:180	arg1	thymus					189:194	the thymus	185:194	the thymus	185:194	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	8	111	theme	inflammation	2353:2364	arg1	focus					2344:2348	the focus	2340:2348	the focus of inflammation in patients with ulcerative colitis	2340:2400	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	4	112	theme	cortical	1005:1012	arg1	substance					1014:1022	the cortical substance	1001:1022	the cortical substance	1001:1022	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	6	113	theme	dendritic	1693:1701	arg1	cells					1703:1707	dendritic cells	1693:1707	dendritic cells	1693:1707	The cyst-like thymic corpuscles formed clusters, the count of dendritic cells increased in early-stage chronic ulcerative colitis, but the levels of macrophages decreased in both periods of its development.					
31626205	0	114	theme	experimental	83:94	arg1	colitis					125:131	experimental acute and chronic ulcerative colitis	83:131	experimental acute and chronic ulcerative colitis	83:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	1	115	theme	ulcerative	362:371	arg1	colitis					373:379	chronic ulcerative colitis	354:379	chronic ulcerative colitis	354:379	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	2	116	theme	sulfate	525:531	arg1	solution					540:547	a 1% aqueous dextran sulfate sodium solution	504:547	a 1% aqueous dextran sulfate sodium solution	504:547	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	0	117	from	features	43:50	arg1	colitis					125:131	experimental acute and chronic ulcerative colitis	83:131	experimental acute and chronic ulcerative colitis	83:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	4	118	from	rise	1312:1315	arg1	index					1324:1328	the index	1320:1328	the index of the area of thymic corpuscles	1320:1361	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	119	theme	T-cells	1234:1240	arg1	number					1185:1190	the absolute number	1172:1190	the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells	1172:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	7	120	theme	thymic	1890:1895	arg1	hyperplasia					1897:1907	thymic hyperplasia	1890:1907	thymic hyperplasia	1890:1907	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	2	121	theme	aqueous	509:515	arg1	solution					540:547	a 1% aqueous dextran sulfate sodium solution	504:547	a 1% aqueous dextran sulfate sodium solution	504:547	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	4	122	theme	lymphocytes	1195:1205	arg1	number					1185:1190	the absolute number	1172:1190	the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells	1172:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	2	123	dep	MATERIAL	382:389	arg1	colitis					432:438	Acute and chronic ulcerative colitis	403:438	Acute and chronic ulcerative colitis	403:438	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	4	124	theme	T-helper	1208:1215	arg1	cells					1217:1221	T-helper cells	1208:1221	T-helper cells	1208:1221	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	4	125	theme	corpuscles	1453:1462	arg1	appearance					1432:1441	the appearance	1428:1441	the appearance of thymic corpuscles as cyst-like cavities	1428:1484	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	0	126	theme	thymus	19:24	arg1	[Morphology					0:10	[Morphology	0:10	[Morphology of the thymus	0:24	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	0	126	theme	thymus	19:24	arg1	features					43:50	the specific features	30:50	the specific features of its cellular composition in experimental acute and chronic ulcerative colitis	30:131	[Morphology of the thymus and the specific features of its cellular composition in experimental acute and chronic ulcerative colitis].					
31626205	1	127	theme	experimental	288:299	arg1	colitis					318:324	dextran-induced experimental acute ulcerative colitis	272:324	dextran-induced experimental acute ulcerative colitis	272:324	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	7	128	theme	acute	1858:1862	arg1	involution					1875:1884	acute accidental involution	1858:1884	acute accidental involution	1858:1884	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	4	129	theme	volume	1050:1055	arg1	fraction					1057:1064	its volume fraction	1046:1064	its volume fraction	1046:1064	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	5	130	theme	ulcerative	1498:1507	arg1	colitis					1509:1515	chronic ulcerative colitis	1490:1515	chronic ulcerative colitis	1490:1515	In chronic ulcerative colitis, the cortex was expanded and the area of thymic corpuscles and the count of medullary epithelial cells increased.					
31626205	3	131	theme	cells	662:666	arg1	number					601:606	number	601:606	number	601:606	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	3	131	theme	cells	662:666	arg1	area					621:624	absolute area	612:624	absolute area	612:624	Thymic changes were morphometrically assessed; the number and absolute area of thymic corpuscles and epithelial cells were calculated; and the subpopulation composition of lymphocytes and thymic stromal cells was determined using flow cytofluorimetry; the Kruskal-Wallis test and the Mann-Whitney test were used to compare the groups.					
31626205	1	132	theme	ulcerative	307:316	arg1	colitis					318:324	dextran-induced experimental acute ulcerative colitis	272:324	dextran-induced experimental acute ulcerative colitis	272:324	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	8	133	located	observed	2304:2311	arg2	number					2285:2290	the larger number	2274:2290	the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature	2274:2429	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	8	133	located	observed	2304:2311	arg1	focus					2344:2348	the focus	2340:2348	the focus of inflammation in patients with ulcerative colitis	2340:2400	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	8	133	located	observed	2304:2311	arg1	blood					2327:2331	peripheral blood	2316:2331	peripheral blood	2316:2331	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	8	133	located	observed	2304:2311	arg2	which					2295:2299	which	2295:2299	which	2295:2299	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	2	134	theme	drinking	484:491	arg1	water					493:497	drinking water	484:497	drinking water	484:497	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	6	135	theme	ulcerative	1742:1751	arg1	colitis					1753:1759	early-stage chronic ulcerative colitis	1722:1759	early-stage chronic ulcerative colitis	1722:1759	The cyst-like thymic corpuscles formed clusters, the count of dendritic cells increased in early-stage chronic ulcerative colitis, but the levels of macrophages decreased in both periods of its development.					
31626205	4	136	theme	dendritic	1288:1296	arg1	cells					1298:1302	dendritic cells	1288:1302	dendritic cells	1288:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	2	137	theme	ulcerative	421:430	arg1	colitis					432:438	Acute and chronic ulcerative colitis	403:438	Acute and chronic ulcerative colitis	403:438	MATERIAL AND METHODS Acute and chronic ulcerative colitis was simulated in C57BL/6 mice, by replacing drinking water with a 1% aqueous dextran sulfate sodium solution.					
31626205	6	138	theme	early-stage	1722:1732	arg1	colitis					1753:1759	early-stage chronic ulcerative colitis	1722:1759	early-stage chronic ulcerative colitis	1722:1759	The cyst-like thymic corpuscles formed clusters, the count of dendritic cells increased in early-stage chronic ulcerative colitis, but the levels of macrophages decreased in both periods of its development.					
31626205	4	139	theme	regulatory	1243:1252	arg1	T-lymphocytes					1254:1266	regulatory T-lymphocytes	1243:1266	regulatory T-lymphocytes	1243:1266	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	1	140	theme	different	333:341	arg1	periods					343:349	different periods	333:349	different periods of chronic ulcerative colitis	333:379	OBJECTIVE To investigate morphological changes in the thymus, the subpopulation composition of lymphocytes and its non-lymphoid cells in dextran-induced experimental acute ulcerative colitis and in different periods of chronic ulcerative colitis.					
31626205	7	141	from	cells	2026:2030	arg1	colitis					2077:2083	experimental acute and chronic ulcerative colitis	2035:2083	experimental acute and chronic ulcerative colitis	2035:2083	CONCLUSION There is acute accidental involution and thymic hyperplasia with an increase in medullary epithelial cells and thymic corpuscles consisting of cytokeratin 19+ in the epithelial cells in experimental acute and chronic ulcerative colitis, respectively.					
31626205	4	142	from	decrease	1160:1167	arg1	number					1185:1190	the absolute number	1172:1190	the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells	1172:1302	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
31626205	8	143	theme	epithelial	2120:2129	arg1	response					2136:2143	The more pronounced epithelial cell response	2100:2143	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis	2100:2202	The more pronounced epithelial cell response found in end-stage experimental chronic ulcerative colitis reflects the enhanced differentiation of regulatory T-lymphocytes and the larger number of which is observed in peripheral blood and in the focus of inflammation in patients with ulcerative colitis, according to the literature.					
31626205	4	144	theme	cyst-like	1467:1475	arg1	cavities					1477:1484	cyst-like cavities	1467:1484	cyst-like cavities	1467:1484	RESULTS In acute catarrhal and ulcerative colitis, there was acute accidental thymic involution with devastation of the cortical substance and with a decline in its volume fraction, with an increase in the levels of cells dying through the mechanism of apoptosis, and with a decrease in the absolute number of lymphocytes, T-helper cells, cytotoxic T-cells, regulatory T-lymphocytes, B-lymphocytes, and dendritic cells, with a rise in the index of the area of thymic corpuscles and in the content of late-phase corpuscles among them, and with the appearance of thymic corpuscles as cyst-like cavities.					
30316609	13	0	theme	different	1965:1973	arg1	latter					1930:1935	latter	1930:1935	latter	1930:1935	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	13	0	theme	different	1965:1973	arg1	breed					1975:1979	the most different breed	1956:1979	the most different breed	1956:1979	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	2	1	theme	Spanish	314:320	arg1	farms					322:326	Spanish farms	314:326	Spanish farms	314:326	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	1	2	theme	breeds	212:217	arg1	use					191:193	the use	187:193	the use of autochthonous breeds	187:217	In Spain, ewe milk is mainly used for cheesemaking, and farming systems have traditionally been based on the use of autochthonous breeds.					
30316609	2	3	theme	selection	436:444	arg1	schemes					446:452	genetic selection schemes	428:452	genetic selection schemes	428:452	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	0	4	from	analysis	13:20	arg1	Spain					75:79	Spain	75:79	Spain	75:79	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.					
30316609	0	4	from	analysis	13:20	arg1	breeds					63:68	ovine breeds	57:68	ovine breeds from Spain	57:79	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.					
30316609	11	5	theme	related	1648:1654	arg1	variables					1638:1646	the variables	1634:1646	the variables related to milk composition	1634:1674	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	13	6	dep	Manchega	1879:1886	arg1	Pedroches					1906:1914	Pedroches	1906:1914	Pedroches	1906:1914	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	8	7	theme	cluster	1144:1150	arg1	analysis					1152:1159	cluster analysis	1144:1159	cluster analysis	1144:1159	In addition, cluster analysis was performed to study and quantify the concrete relationships among the discriminated groups.					
30316609	2	8	theme	milk	486:489	arg1	aptitude					505:512	milk technological aptitude	486:512	milk technological aptitude	486:512	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	11	9	theme	Merino	1509:1514	arg1	Grazalema					1519:1527	Merino de Grazalema	1509:1527	Merino de Grazalema	1509:1527	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	7	10	theme	discriminant	1007:1018	arg1	analysis					1020:1027	Canonical discriminant analysis	997:1027	Canonical discriminant analysis	997:1027	Canonical discriminant analysis was performed to establish differences and similarities among breeds based on the measured variables.					
30316609	4	11	from	breeds	709:714	arg1	samples					692:698	832 individual ewe milk samples	668:698	832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	668:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	2	12	from	introduction	262:273	arg1	farms					322:326	Spanish farms	314:326	Spanish farms	314:326	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	13	13	theme	Official	2073:2080	arg1	Catalogue					2082:2090	the Official Catalogue	2069:2090	the Official Catalogue of Spanish Livestock Breeds	2069:2118	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	13	14	from	breed	2060:2064	arg1	Catalogue					2082:2090	the Official Catalogue	2069:2090	the Official Catalogue of Spanish Livestock Breeds	2069:2118	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	11	15	theme	higher	1600:1605	arg1	ability					1622:1628	a higher discriminating ability	1598:1628	a higher discriminating ability for the variables related to milk composition	1598:1674	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	4	16	dep	de	765:766	arg1	Los					768:770	Los	768:770	Los	768:770	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	11	17	theme	milk	1659:1662	arg1	composition					1664:1674	milk composition	1659:1674	milk composition	1659:1674	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	0	18	theme	Multivariate	0:11	arg1	analysis					13:20	Multivariate analysis	0:20	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.	0:80	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.					
30316609	2	19	theme	breeds	304:309	arg1	introduction					262:273	the progressive introduction	246:273	the progressive introduction of highly productive foreign breeds in Spanish farms	246:326	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	4	20	theme	de	765:766	arg1	Pedroches					772:780	Merino de Los Pedroches	758:780	Merino de Los Pedroches	758:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	4	20	theme	de	765:766	arg1	breeds					709:714	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	11	21	theme	Cluster	1458:1464	arg1	analysis					1466:1473	Cluster analysis	1458:1473	Cluster analysis	1458:1473	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	2	22	theme	recent	232:237	arg1	years					239:243	recent years	232:243	recent years	232:243	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	2	23	theme	increasing	342:351	arg1	interest					353:360	an increasing interest	339:360	an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude	339:512	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	2	24	theme	genetic	428:434	arg1	schemes					446:452	genetic selection schemes	428:452	genetic selection schemes	428:452	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	13	25	theme	Merino	1892:1897	arg1	de					1899:1900	Merino de	1892:1900	Merino de	1892:1900	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	1	26	used	used	111:114	arg2	milk					96:99	ewe milk	92:99	ewe milk	92:99	In Spain, ewe milk is mainly used for cheesemaking, and farming systems have traditionally been based on the use of autochthonous breeds.					
30316609	10	27	from	evident	1401:1407	arg1	cases					1362:1366	all cases	1358:1366	all cases	1358:1366	In all cases, discrimination among breeds was evident and the 4 breeds could be easily differentiated.					
30316609	1	28	theme	farming	138:144	arg1	systems					146:152	farming systems	138:152	farming systems	138:152	In Spain, ewe milk is mainly used for cheesemaking, and farming systems have traditionally been based on the use of autochthonous breeds.					
30316609	2	29	theme	breeds	401:406	arg1	characterization					369:384	the characterization	365:384	the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude	365:512	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	10	30	from	cases	1362:1366	arg1	evident					1401:1407	evident	1401:1407	evident	1401:1407	In all cases, discrimination among breeds was evident and the 4 breeds could be easily differentiated.					
30316609	4	31	theme	ewe	683:685	arg1	samples					692:698	832 individual ewe milk samples	668:698	832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	668:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	11	32	theme	discriminating	1607:1620	arg1	ability					1622:1628	a higher discriminating ability	1598:1628	a higher discriminating ability for the variables related to milk composition	1598:1674	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	6	33	theme	coagulation	953:963	arg1	efficiency					965:974	coagulation efficiency	953:974	coagulation efficiency	953:974	An indicator of coagulation efficiency was also determined.					
30316609	2	34	theme	sheep	395:399	arg1	breeds					401:406	dairy sheep breeds	389:406	dairy sheep breeds	389:406	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	9	35	theme	Discriminant	1256:1267	arg1	analysis					1269:1276	Discriminant analysis	1256:1276	Discriminant analysis	1256:1276	Discriminant analysis proved to be a powerful tool to accurately draw distinctions between breeds.					
30316609	9	35	theme	Discriminant	1256:1267	arg1	tool					1302:1305	a powerful tool	1291:1305	a powerful tool to accurately draw distinctions between breeds	1291:1352	Discriminant analysis proved to be a powerful tool to accurately draw distinctions between breeds.					
30316609	11	36	theme	greater	1482:1488	arg1	similarity					1490:1499	greater similarity	1482:1499	greater similarity between Merino de Grazalema and Assaf	1482:1537	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	3	37	theme	work	535:538	arg1	purpose					519:525	The purpose	515:525	The purpose of this work	515:538	The purpose of this work was to explore milk composition and coagulation to classify 4 of the main dairy sheep breeds used in Spain.					
30316609	2	38	theme	dairy	389:393	arg1	breeds					401:406	dairy sheep breeds	389:406	dairy sheep breeds	389:406	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	5	39	theme	curd	925:928	arg1	yield					930:934	individual laboratory curd yield	903:934	individual laboratory curd yield	903:934	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	2	40	theme	progressive	250:260	arg1	introduction					262:273	the progressive introduction	246:273	the progressive introduction of highly productive foreign breeds in Spanish farms	246:326	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	7	41	theme	Canonical	997:1005	arg1	analysis					1020:1027	Canonical discriminant analysis	997:1027	Canonical discriminant analysis	997:1027	Canonical discriminant analysis was performed to establish differences and similarities among breeds based on the measured variables.					
30316609	4	42	theme	individual	672:681	arg1	samples					692:698	832 individual ewe milk samples	668:698	832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	668:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	5	43	dep	composition	820:830	arg1	lactose					847:853	lactose	847:853	lactose	847:853	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	5	43	dep	composition	820:830	arg1	fat					833:835	fat	833:835	fat	833:835	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	5	43	dep	composition	820:830	arg1	protein					838:844	protein	838:844	protein	838:844	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	5	43	dep	composition	820:830	arg1	solids					866:871	total solids	860:871	total solids	860:871	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	0	44	from	Spain	75:79	arg1	analysis					13:20	Multivariate analysis	0:20	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.	0:80	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.					
30316609	0	44	from	Spain	75:79	arg1	breeds					63:68	ovine breeds	57:68	ovine breeds from Spain	57:79	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.					
30316609	4	45	theme	milk	687:690	arg1	samples					692:698	832 individual ewe milk samples	668:698	832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	668:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	5	46	theme	total	860:864	arg1	solids					866:871	total solids	860:871	total solids	860:871	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	0	47	theme	coagulation	34:44	arg1	process					46:52	the milk coagulation process	25:52	the milk coagulation process	25:52	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.					
30316609	11	48	dep	showed	1475:1480	arg1	compared					1539:1546	compared	1539:1546	showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds	1475:1568	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	0	49	theme	milk	29:32	arg1	process					46:52	the milk coagulation process	25:52	the milk coagulation process	25:52	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.					
30316609	2	50	theme	technological	491:503	arg1	aptitude					505:512	milk technological aptitude	486:512	milk technological aptitude	486:512	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	13	51	theme	independent	2048:2058	arg1	ground					2011:2016	the ground	2007:2016	the ground for its differentiation	2007:2040	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	13	51	theme	independent	2048:2058	arg1	breed					2060:2064	an independent breed	2045:2064	an independent breed in the Official Catalogue of Spanish Livestock Breeds	2045:2118	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	3	52	theme	milk	555:558	arg1	composition					560:570	milk composition	555:570	milk composition	555:570	The purpose of this work was to explore milk composition and coagulation to classify 4 of the main dairy sheep breeds used in Spain.					
30316609	13	53	theme	all	1984:1986	arg1	latter					1930:1935	latter	1930:1935	latter	1930:1935	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	13	53	theme	all	1984:1986	arg1	breed					1975:1979	the most different breed	1956:1979	the most different breed	1956:1979	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	8	54	theme	concrete	1201:1208	arg1	relationships					1210:1222	the concrete relationships	1197:1222	the concrete relationships among the discriminated groups	1197:1253	In addition, cluster analysis was performed to study and quantify the concrete relationships among the discriminated groups.					
30316609	7	55	theme	measured	1111:1118	arg1	variables					1120:1128	the measured variables	1107:1128	the measured variables	1107:1128	Canonical discriminant analysis was performed to establish differences and similarities among breeds based on the measured variables.					
30316609	5	56	theme	laboratory	914:923	arg1	yield					930:934	individual laboratory curd yield	903:934	individual laboratory curd yield	903:934	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	12	57	theme	coagulation	1785:1795	arg1	process					1797:1803	the coagulation process	1781:1803	the coagulation process	1781:1803	However, Merino de Grazalema and Manchega were difficult to separate according to milk composition, but the coagulation process differenced them clearly.					
30316609	1	58	theme	ewe	92:94	arg1	milk					96:99	ewe milk	92:99	ewe milk	92:99	In Spain, ewe milk is mainly used for cheesemaking, and farming systems have traditionally been based on the use of autochthonous breeds.					
30316609	0	59	theme	process	46:52	arg1	analysis					13:20	Multivariate analysis	0:20	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.	0:80	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.					
30316609	9	60	theme	powerful	1293:1300	arg1	analysis					1269:1276	Discriminant analysis	1256:1276	Discriminant analysis	1256:1276	Discriminant analysis proved to be a powerful tool to accurately draw distinctions between breeds.					
30316609	9	60	theme	powerful	1293:1300	arg1	tool					1302:1305	a powerful tool	1291:1305	a powerful tool to accurately draw distinctions between breeds	1291:1352	Discriminant analysis proved to be a powerful tool to accurately draw distinctions between breeds.					
30316609	3	61	theme	sheep	620:624	arg1	breeds					626:631	the main dairy sheep breeds	605:631	the main dairy sheep breeds used in Spain	605:645	The purpose of this work was to explore milk composition and coagulation to classify 4 of the main dairy sheep breeds used in Spain.					
30316609	4	62	theme	Merino	758:763	arg1	Pedroches					772:780	Merino de Los Pedroches	758:780	Merino de Los Pedroches	758:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	4	62	theme	Merino	758:763	arg1	breeds					709:714	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	11	63	theme	de	1516:1517	arg1	Grazalema					1519:1527	Merino de Grazalema	1509:1527	Merino de Grazalema	1509:1527	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	2	64	theme	foreign	296:302	arg1	breeds					304:309	highly productive foreign breeds	278:309	highly productive foreign breeds	278:309	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	11	65	theme	other	1557:1561	arg1	breeds					1563:1568	the other breeds	1553:1568	the other breeds	1553:1568	Cluster analysis showed greater similarity between Merino de Grazalema and Assaf compared with the other breeds, and F-statistics indicated a higher discriminating ability for the variables related to milk composition.					
30316609	5	66	theme	individual	903:912	arg1	yield					930:934	individual laboratory curd yield	903:934	individual laboratory curd yield	903:934	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	3	67	theme	breeds	626:631	arg1	4					600:600	4	600:600	4	600:600	The purpose of this work was to explore milk composition and coagulation to classify 4 of the main dairy sheep breeds used in Spain.					
30316609	3	67	theme	breeds	626:631	arg1	breeds					626:631	the main dairy sheep breeds	605:631	the main dairy sheep breeds used in Spain	605:645	The purpose of this work was to explore milk composition and coagulation to classify 4 of the main dairy sheep breeds used in Spain.					
30316609	4	68	theme	de	740:741	arg1	Grazalema					743:751	Merino de Grazalema	733:751	Merino de Grazalema	733:751	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	4	68	theme	de	740:741	arg1	breeds					709:714	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	2	69	theme	productive	285:294	arg1	breeds					304:309	highly productive foreign breeds	278:309	highly productive foreign breeds	278:309	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	12	70	theme	milk	1759:1762	arg1	composition					1764:1774	milk composition	1759:1774	milk composition	1759:1774	However, Merino de Grazalema and Manchega were difficult to separate according to milk composition, but the coagulation process differenced them clearly.					
30316609	3	71	theme	main	609:612	arg1	breeds					626:631	the main dairy sheep breeds	605:631	the main dairy sheep breeds used in Spain	605:645	The purpose of this work was to explore milk composition and coagulation to classify 4 of the main dairy sheep breeds used in Spain.					
30316609	5	72	theme	coagulation	875:885	arg1	properties					887:896	coagulation properties	875:896	coagulation properties	875:896	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	5	73	theme	native	809:814	arg1	pH					816:817	native pH	809:817	native pH	809:817	Samples were analyzed for native pH, composition (fat, protein, lactose, and total solids), coagulation properties, and individual laboratory curd yield.					
30316609	13	74	theme	Spanish	2095:2101	arg1	Breeds					2113:2118	Spanish Livestock Breeds	2095:2118	Spanish Livestock Breeds	2095:2118	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	3	75	theme	dairy	614:618	arg1	breeds					626:631	the main dairy sheep breeds	605:631	the main dairy sheep breeds used in Spain	605:645	The purpose of this work was to explore milk composition and coagulation to classify 4 of the main dairy sheep breeds used in Spain.					
30316609	4	76	theme	Merino	733:738	arg1	Grazalema					743:751	Merino de Grazalema	733:751	Merino de Grazalema	733:751	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	4	76	theme	Merino	733:738	arg1	breeds					709:714	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	2	77	from	interest	353:360	arg1	characterization					369:384	the characterization	365:384	the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude	365:512	However, in recent years, the progressive introduction of highly productive foreign breeds in Spanish farms has led to an increasing interest in the characterization of dairy sheep breeds to evaluate whether genetic selection schemes should focus on productivity or milk technological aptitude.					
30316609	8	78	theme	discriminated	1234:1246	arg1	groups					1248:1253	the discriminated groups	1230:1253	the discriminated groups	1230:1253	In addition, cluster analysis was performed to study and quantify the concrete relationships among the discriminated groups.					
30316609	6	79	theme	efficiency	965:974	arg1	indicator					940:948	An indicator	937:948	An indicator of coagulation efficiency	937:974	An indicator of coagulation efficiency was also determined.					
30316609	13	80	theme	Livestock	2103:2111	arg1	Breeds					2113:2118	Spanish Livestock Breeds	2095:2118	Spanish Livestock Breeds	2095:2118	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	0	81	theme	ovine	57:61	arg1	breeds					63:68	ovine breeds	57:68	ovine breeds from Spain	57:79	Multivariate analysis of the milk coagulation process in ovine breeds from Spain.					
30316609	13	82	theme	Breeds	2113:2118	arg1	Catalogue					2082:2090	the Official Catalogue	2069:2090	the Official Catalogue of Spanish Livestock Breeds	2069:2118	Coagulation also evidenced similarities between Manchega and Merino de Los Pedroches, although the latter was revealed to be the most different breed of all 4, which could lay the ground for its differentiation as an independent breed in the Official Catalogue of Spanish Livestock Breeds.					
30316609	4	83	dep	breeds	709:714	arg1	Grazalema					743:751	Merino de Grazalema	733:751	Merino de Grazalema	733:751	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	4	83	dep	breeds	709:714	arg1	Pedroches					772:780	Merino de Los Pedroches	758:780	Merino de Los Pedroches	758:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	4	83	dep	breeds	709:714	arg1	breeds					709:714	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches	705:780	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	4	83	dep	breeds	709:714	arg1	Assaf					726:730	Assaf	726:730	Assaf	726:730	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	4	83	dep	breeds	709:714	arg1	Manchega					716:723	Manchega	716:723	Manchega	716:723	This study included 832 individual ewe milk samples from the breeds Manchega, Assaf, Merino de Grazalema, and Merino de Los Pedroches.					
30316609	1	84	theme	autochthonous	198:210	arg1	breeds					212:217	autochthonous breeds	198:217	autochthonous breeds	198:217	In Spain, ewe milk is mainly used for cheesemaking, and farming systems have traditionally been based on the use of autochthonous breeds.					
31227161	0	0	theme	composite	59:67	arg1	microspheres					69:80	composite microspheres	59:80	composite microspheres	59:80	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	1	1	theme	UV	322:323	arg1	reduction					325:333	the in situ UV reduction	310:333	the in situ UV reduction	310:333	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	4	2	theme	in-situ	717:723	arg1	reduction					728:736	the in-situ UV reduction	713:736	the in-situ UV reduction	713:736	Additionally, the AgNPs prepared by the in-situ UV reduction exhibited better biocompatibility and antibacterial effects than those prepared by the conventional chemical reduction method.					
31227161	6	3	theme	swelling	1057:1064	arg1	properties					1066:1075	pH-sensitive swelling properties	1044:1075	pH-sensitive swelling properties	1044:1075	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	4	theme	Such	976:979	arg1	network					1031:1037	a porous and hollow network	1011:1037	a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance	1011:1112	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	4	theme	Such	976:979	arg1	microspheres					981:992	Such microspheres	976:992	Such microspheres	976:992	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	5	theme	hemostatic	1182:1191	arg1	potentials					1142:1151	its application potentials	1126:1151	its application potentials	1126:1151	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	5	theme	hemostatic	1182:1191	arg1	material					1193:1200	an advanced antibacterial hemostatic material	1156:1200	an advanced antibacterial hemostatic material	1156:1200	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	3	6	theme	gram-positive	653:665	arg1	bacteria					667:674	both gram-negative and gram-positive bacteria	630:674	both gram-negative and gram-positive bacteria	630:674	The AgNPs showed excellent antibacterial activities against both gram-negative and gram-positive bacteria.					
31227161	6	7	theme	pH-sensitive	1044:1055	arg1	properties					1066:1075	pH-sensitive swelling properties	1044:1075	pH-sensitive swelling properties	1044:1075	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	8	theme	antibacterial	1168:1180	arg1	potentials					1142:1151	its application potentials	1126:1151	its application potentials	1126:1151	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	8	theme	antibacterial	1168:1180	arg1	material					1193:1200	an advanced antibacterial hemostatic material	1156:1200	an advanced antibacterial hemostatic material	1156:1200	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	9	with	network	1031:1037	arg1	performance					1102:1112	excellent hemostatic performance	1081:1112	excellent hemostatic performance	1081:1112	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	9	with	network	1031:1037	arg1	properties					1066:1075	pH-sensitive swelling properties	1044:1075	pH-sensitive swelling properties	1044:1075	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	1	10	theme	excellent	244:252	arg1	properties					283:292	excellent antibacterial and hemostatic properties	244:292	excellent antibacterial and hemostatic properties	244:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	2	11	theme	reduction	510:518	arg1	method					520:525	a UV reduction method	505:525	a UV reduction method with alginate	505:539	Well dispersed AgNPs were in situ synthesized by a UV reduction method with alginate as stabilizer and reductant.					
31227161	1	12	theme	emulsion	339:346	arg1	method					366:371	emulsion internal gelation method	339:371	emulsion internal gelation method	339:371	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	5	13	theme	internal	950:957	arg1	method					968:973	an emulsion internal gelation method	938:973	an emulsion internal gelation method	938:973	PGA/Alg/AgNP composite microspheres were then prepared with the AgNPs by an emulsion internal gelation method.					
31227161	1	14	theme	antibacterial	254:266	arg1	properties					283:292	excellent antibacterial and hemostatic properties	244:292	excellent antibacterial and hemostatic properties	244:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	2	15	theme	UV	507:508	arg1	method					520:525	a UV reduction method	505:525	a UV reduction method with alginate	505:539	Well dispersed AgNPs were in situ synthesized by a UV reduction method with alginate as stabilizer and reductant.					
31227161	1	16	theme	internal	348:355	arg1	method					366:371	emulsion internal gelation method	339:371	emulsion internal gelation method	339:371	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	0	17	theme	antibacterial	87:99	arg1	properties					116:125	antibacterial and hemostatic properties	87:125	antibacterial and hemostatic properties	87:125	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	1	18	theme	gelation	357:364	arg1	method					366:371	emulsion internal gelation method	339:371	emulsion internal gelation method	339:371	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	6	19	theme	excellent	1081:1089	arg1	performance					1102:1112	excellent hemostatic performance	1081:1112	excellent hemostatic performance	1081:1112	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	20	theme	advanced	1159:1166	arg1	potentials					1142:1151	its application potentials	1126:1151	its application potentials	1126:1151	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	20	theme	advanced	1159:1166	arg1	material					1193:1200	an advanced antibacterial hemostatic material	1156:1200	an advanced antibacterial hemostatic material	1156:1200	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	1	21	theme	poly	151:154	arg1	microsphere					227:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere	149:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties	149:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	1	22	dep	in	314:315	arg1	situ					317:320	situ	317:320	situ	317:320	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	0	23	theme	In	0:1	arg1	synthesis					8:16	In situ synthesis	0:16	In situ synthesis of poly (γ- glutamic acid)	0:43	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	6	24	theme	hollow	1024:1029	arg1	network					1031:1037	a porous and hollow network	1011:1037	a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance	1011:1112	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	24	theme	hollow	1024:1029	arg1	microspheres					981:992	Such microspheres	976:992	Such microspheres	976:992	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	2	25	with	method	520:525	arg1	alginate					532:539	alginate	532:539	alginate	532:539	Well dispersed AgNPs were in situ synthesized by a UV reduction method with alginate as stabilizer and reductant.					
31227161	1	26	theme	γ-glutamic	156:165	arg1	poly					151:154	poly	151:154	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties	149:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	1	26	theme	γ-glutamic	156:165	arg1	acid					167:170	γ-glutamic acid	156:170	γ-glutamic acid	156:170	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	5	27	theme	PGA/Alg/AgNP	865:876	arg1	microspheres					888:899	PGA/Alg/AgNP composite microspheres	865:899	PGA/Alg/AgNP composite microspheres	865:899	PGA/Alg/AgNP composite microspheres were then prepared with the AgNPs by an emulsion internal gelation method.					
31227161	1	28	theme	hemostatic	272:281	arg1	properties					283:292	excellent antibacterial and hemostatic properties	244:292	excellent antibacterial and hemostatic properties	244:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	4	29	theme	chemical	838:845	arg1	method					857:862	the conventional chemical reduction method	821:862	the conventional chemical reduction method	821:862	Additionally, the AgNPs prepared by the in-situ UV reduction exhibited better biocompatibility and antibacterial effects than those prepared by the conventional chemical reduction method.					
31227161	6	30	theme	porous	1013:1018	arg1	network					1031:1037	a porous and hollow network	1011:1037	a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance	1011:1112	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	30	theme	porous	1013:1018	arg1	microspheres					981:992	Such microspheres	976:992	Such microspheres	976:992	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	0	31	theme	hemostatic	105:114	arg1	properties					116:125	antibacterial and hemostatic properties	87:125	antibacterial and hemostatic properties	87:125	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	4	32	theme	antibacterial	776:788	arg1	effects					790:796	antibacterial effects	776:796	antibacterial effects	776:796	Additionally, the AgNPs prepared by the in-situ UV reduction exhibited better biocompatibility and antibacterial effects than those prepared by the conventional chemical reduction method.					
31227161	3	33	theme	excellent	587:595	arg1	activities					611:620	excellent antibacterial activities	587:620	excellent antibacterial activities against both gram-negative and gram-positive bacteria	587:674	The AgNPs showed excellent antibacterial activities against both gram-negative and gram-positive bacteria.					
31227161	6	34	theme	application	1130:1140	arg1	potentials					1142:1151	its application potentials	1126:1151	its application potentials	1126:1151	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	6	34	theme	application	1130:1140	arg1	material					1193:1200	an advanced antibacterial hemostatic material	1156:1200	an advanced antibacterial hemostatic material	1156:1200	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	1	35	theme	/alginate/silver	172:187	arg1	microsphere					227:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere	149:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties	149:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	0	36	theme	poly	21:24	arg1	synthesis					8:16	In situ synthesis	0:16	In situ synthesis of poly (γ- glutamic acid)	0:43	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	3	37	theme	antibacterial	597:609	arg1	activities					611:620	excellent antibacterial activities	587:620	excellent antibacterial activities against both gram-negative and gram-positive bacteria	587:674	The AgNPs showed excellent antibacterial activities against both gram-negative and gram-positive bacteria.					
31227161	1	38	theme	nanoparticle	189:200	arg1	microsphere					227:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere	149:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties	149:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	0	39	theme	glutamic	30:37	arg1	acid					39:42	γ- glutamic acid	27:42	γ- glutamic acid	27:42	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	0	39	theme	glutamic	30:37	arg1	poly					21:24	poly	21:24	poly (γ- glutamic acid)	21:43	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	5	40	theme	composite	878:886	arg1	microspheres					888:899	PGA/Alg/AgNP composite microspheres	865:899	PGA/Alg/AgNP composite microspheres	865:899	PGA/Alg/AgNP composite microspheres were then prepared with the AgNPs by an emulsion internal gelation method.					
31227161	1	41	theme	potential	382:390	arg1	application					392:402	its potential application	378:402	its potential application for antibacterial hemostatic dressing	378:440	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	0	42	theme	γ-	27:28	arg1	acid					39:42	γ- glutamic acid	27:42	γ- glutamic acid	27:42	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	0	42	theme	γ-	27:28	arg1	poly					21:24	poly	21:24	poly (γ- glutamic acid)	21:43	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	1	43	with	microsphere	227:237	arg1	properties					283:292	excellent antibacterial and hemostatic properties	244:292	excellent antibacterial and hemostatic properties	244:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	4	44	theme	UV	725:726	arg1	reduction					728:736	the in-situ UV reduction	713:736	the in-situ UV reduction	713:736	Additionally, the AgNPs prepared by the in-situ UV reduction exhibited better biocompatibility and antibacterial effects than those prepared by the conventional chemical reduction method.					
31227161	1	45	theme	in	314:315	arg1	reduction					325:333	the in situ UV reduction	310:333	the in situ UV reduction	310:333	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	4	46	theme	conventional	825:836	arg1	method					857:862	the conventional chemical reduction method	821:862	the conventional chemical reduction method	821:862	Additionally, the AgNPs prepared by the in-situ UV reduction exhibited better biocompatibility and antibacterial effects than those prepared by the conventional chemical reduction method.					
31227161	5	47	theme	gelation	959:966	arg1	method					968:973	an emulsion internal gelation method	938:973	an emulsion internal gelation method	938:973	PGA/Alg/AgNP composite microspheres were then prepared with the AgNPs by an emulsion internal gelation method.					
31227161	6	48	theme	hemostatic	1091:1100	arg1	performance					1102:1112	excellent hemostatic performance	1081:1112	excellent hemostatic performance	1081:1112	Such microspheres were found to be a porous and hollow network with pH-sensitive swelling properties and excellent hemostatic performance, indicating its application potentials as an advanced antibacterial hemostatic material.					
31227161	1	49	theme	antibacterial	408:420	arg1	dressing					433:440	antibacterial hemostatic dressing	408:440	antibacterial hemostatic dressing	408:440	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	3	50	theme	gram-negative	635:647	arg1	bacteria					667:674	both gram-negative and gram-positive bacteria	630:674	both gram-negative and gram-positive bacteria	630:674	The AgNPs showed excellent antibacterial activities against both gram-negative and gram-positive bacteria.					
31227161	5	51	theme	emulsion	941:948	arg1	method					968:973	an emulsion internal gelation method	938:973	an emulsion internal gelation method	938:973	PGA/Alg/AgNP composite microspheres were then prepared with the AgNPs by an emulsion internal gelation method.					
31227161	1	52	theme	PGA/Alg/AgNP	203:214	arg1	microsphere					227:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere	149:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties	149:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	1	53	theme	hemostatic	422:431	arg1	dressing					433:440	antibacterial hemostatic dressing	408:440	antibacterial hemostatic dressing	408:440	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	4	54	theme	reduction	847:855	arg1	method					857:862	the conventional chemical reduction method	821:862	the conventional chemical reduction method	821:862	Additionally, the AgNPs prepared by the in-situ UV reduction exhibited better biocompatibility and antibacterial effects than those prepared by the conventional chemical reduction method.					
31227161	2	55	theme	dispersed	461:469	arg1	reductant					559:567	reductant	559:567	reductant	559:567	Well dispersed AgNPs were in situ synthesized by a UV reduction method with alginate as stabilizer and reductant.					
31227161	2	55	theme	dispersed	461:469	arg1	stabilizer					544:553	stabilizer	544:553	stabilizer	544:553	Well dispersed AgNPs were in situ synthesized by a UV reduction method with alginate as stabilizer and reductant.					
31227161	2	55	theme	dispersed	461:469	arg1	AgNPs					471:475	Well dispersed AgNPs	456:475	Well dispersed AgNPs	456:475	Well dispersed AgNPs were in situ synthesized by a UV reduction method with alginate as stabilizer and reductant.					
31227161	0	56	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ synthesis of poly (γ- glutamic acid)/alginate/AgNP composite microspheres with antibacterial and hemostatic properties.					
31227161	1	57	theme	present	135:141	arg1	work					143:146	the present work	131:146	the present work	131:146	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
31227161	1	58	theme	composite	217:225	arg1	microsphere					227:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere	149:237	a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties	149:292	In the present work, a poly(γ-glutamic acid)/alginate/silver nanoparticle (PGA/Alg/AgNP) composite microsphere with excellent antibacterial and hemostatic properties was prepared by the in situ UV reduction and emulsion internal gelation method, and its potential application for antibacterial hemostatic dressing was explored.					
30113166	1	0	from	doses	403:407	arg1	activity					457:464	its metabolic activity	443:464	its metabolic activity	443:464	Fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were employed as substrates for in vitro fermentations to assess their capacity to counteract the effects caused by three antibiotics (ABs) at different doses on the elderly gut microbiota and its metabolic activity.					
30113166	1	0	from	doses	403:407	arg1	microbiota					428:437	the elderly gut microbiota	412:437	the elderly gut microbiota	412:437	Fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were employed as substrates for in vitro fermentations to assess their capacity to counteract the effects caused by three antibiotics (ABs) at different doses on the elderly gut microbiota and its metabolic activity.					
30113166	2	1	theme	microbiota	542:551	arg1	composition					553:563	the microbiota composition	538:563	the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1)	538:705	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	2	from	presence	813:820	arg1	%					849:849	27.5% to 55.7%	836:849	27.5% to 55.7%	836:849	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	3	theme	XOS1	825:828	arg1	presence					813:820	the presence	809:820	the presence of XOS1 (from 27.5% to 55.7%)	809:850	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	0	4	theme	Undesirable	93:103	arg1	Effects					105:111	the Undesirable Effects	89:111	the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota	89:160	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	1	5	dep	in	282:283	arg1	vitro					285:289	vitro	285:289	vitro	285:289	Fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were employed as substrates for in vitro fermentations to assess their capacity to counteract the effects caused by three antibiotics (ABs) at different doses on the elderly gut microbiota and its metabolic activity.					
30113166	1	6	theme	gut	424:426	arg1	microbiota					428:437	the elderly gut microbiota	412:437	the elderly gut microbiota	412:437	Fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were employed as substrates for in vitro fermentations to assess their capacity to counteract the effects caused by three antibiotics (ABs) at different doses on the elderly gut microbiota and its metabolic activity.					
30113166	4	7	theme	butyrate	1174:1181	arg1	production					1155:1164	the production	1151:1164	the production of both butyrate and acetate	1151:1193	XOS2 (mainly DP = 2-4) also showed ability to increase the percentages of Bifidobacterium and the production of both butyrate and acetate.					
30113166	4	7	theme	butyrate	1174:1181	arg1	Bifidobacterium					1131:1145	Bifidobacterium	1131:1145	Bifidobacterium	1131:1145	XOS2 (mainly DP = 2-4) also showed ability to increase the percentages of Bifidobacterium and the production of both butyrate and acetate.					
30113166	0	8	theme	Several	116:122	arg1	Antibiotics					124:134	Several Antibiotics	116:134	Several Antibiotics	116:134	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	2	9	dep	%	849:849	arg1	to					842:843	to	842:843	to	842:843	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	10	theme	relative	614:621	arg1	percentages					623:633	the relative percentages	610:633	the relative percentages of Lactobacillus	610:650	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	3	11	theme	Lactobacillus	951:963	arg1	production					922:931	the butyrate production	909:931	the butyrate production	909:931	FOS were able to counteract these effects by increasing the butyrate production and the number of Lactobacillus, while maintaining the number of Bacteroides almost constant and decreasing the clostridia.					
30113166	3	11	theme	Lactobacillus	951:963	arg1	number					941:946	the number	937:946	the number of Lactobacillus	937:963	FOS were able to counteract these effects by increasing the butyrate production and the number of Lactobacillus, while maintaining the number of Bacteroides almost constant and decreasing the clostridia.					
30113166	4	12	theme	Bifidobacterium	1131:1145	arg1	percentages					1116:1126	the percentages	1112:1126	the percentages of Bifidobacterium and the production of both butyrate and acetate	1112:1193	XOS2 (mainly DP = 2-4) also showed ability to increase the percentages of Bifidobacterium and the production of both butyrate and acetate.					
30113166	2	13	dep	%	680:680	arg1	to					674:675	to	674:675	to	674:675	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	14	theme	ABs	605:607	arg1	presence					593:600	the presence	589:600	the presence of ABs	589:607	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	15	theme	XOS1	701:704	arg1	XOS1					701:704	XOS1	701:704	XOS1	701:704	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	15	theme	XOS1	701:704	arg1	%					680:680	11.4% to 3.2%	668:680	11.4% to 3.2% in the presence of XOS1	668:704	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	0	16	theme	Antibiotics	124:134	arg1	Effects					105:111	the Undesirable Effects	89:111	the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota	89:160	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	1	17	theme	metabolic	447:455	arg1	activity					457:464	its metabolic activity	443:464	its metabolic activity	443:464	Fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were employed as substrates for in vitro fermentations to assess their capacity to counteract the effects caused by three antibiotics (ABs) at different doses on the elderly gut microbiota and its metabolic activity.					
30113166	2	18	theme	AB	471:472	arg1	type					474:477	The AB type	467:477	The AB type	467:477	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	1	19	theme	in	282:283	arg1	fermentations					291:303	in vitro fermentations	282:303	in vitro fermentations	282:303	Fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were employed as substrates for in vitro fermentations to assess their capacity to counteract the effects caused by three antibiotics (ABs) at different doses on the elderly gut microbiota and its metabolic activity.					
30113166	4	20	dep	XOS2	1057:1060	arg1	DP					1070:1071	DP	1070:1071	mainly DP = 2-4	1063:1077	XOS2 (mainly DP = 2-4) also showed ability to increase the percentages of Bifidobacterium and the production of both butyrate and acetate.					
30113166	2	21	theme	%	840:840	arg1	%					849:849	27.5% to 55.7%	836:849	27.5% to 55.7%	836:849	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	22	from	%	849:849	arg1	presence					813:820	the presence	809:820	the presence of XOS1 (from 27.5% to 55.7%)	809:850	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	22	from	%	849:849	arg1	XOS1					825:828	XOS1	825:828	XOS1 (from 27.5% to 55.7%)	825:850	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	23	theme	Lactobacillus	638:650	arg1	percentages					623:633	the relative percentages	610:633	the relative percentages of Lactobacillus	610:650	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	0	24	theme	Fecal	145:149	arg1	Microbiota					151:160	Elder Fecal Microbiota	139:160	Elder Fecal Microbiota	139:160	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	2	25	from	%	680:680	arg1	presence					689:696	the presence	685:696	the presence	685:696	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	0	26	theme	Fructooligosaccharides	13:34	arg1	Potential					0:8	Potential	0:8	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota	0:160	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	0	27	theme	Elder	139:143	arg1	Microbiota					151:160	Elder Fecal Microbiota	139:160	Elder Fecal Microbiota	139:160	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	0	28	from	Effects	105:111	arg1	Microbiota					151:160	Elder Fecal Microbiota	139:160	Elder Fecal Microbiota	139:160	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	2	29	dep	h.	574:575	arg1	decreased					652:660	decreased	652:660	decreased (from 11.4% to 3.2% in the presence of XOS1)	652:705	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	3	30	theme	Bacteroides	998:1008	arg1	number					988:993	the number	984:993	the number of Bacteroides almost constant	984:1024	FOS were able to counteract these effects by increasing the butyrate production and the number of Lactobacillus, while maintaining the number of Bacteroides almost constant and decreasing the clostridia.					
30113166	4	31	theme	production	1155:1164	arg1	percentages					1116:1126	the percentages	1112:1126	the percentages of Bifidobacterium and the production of both butyrate and acetate	1112:1193	XOS2 (mainly DP = 2-4) also showed ability to increase the percentages of Bifidobacterium and the production of both butyrate and acetate.					
30113166	4	32	dep	=	1073:1073	arg1	2-4					1075:1077	2-4	1075:1077	2-4	1075:1077	XOS2 (mainly DP = 2-4) also showed ability to increase the percentages of Bifidobacterium and the production of both butyrate and acetate.					
30113166	0	33	theme	Xylooligosaccharides	40:59	arg1	Potential					0:8	Potential	0:8	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota	0:160	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	2	34	theme	bacterial	516:524	arg1	numbers					526:532	the total bacterial numbers	506:532	the total bacterial numbers	506:532	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	4	35	theme	acetate	1187:1193	arg1	production					1155:1164	the production	1151:1164	the production of both butyrate and acetate	1151:1193	XOS2 (mainly DP = 2-4) also showed ability to increase the percentages of Bifidobacterium and the production of both butyrate and acetate.					
30113166	4	35	theme	acetate	1187:1193	arg1	Bifidobacterium					1131:1145	Bifidobacterium	1131:1145	Bifidobacterium	1131:1145	XOS2 (mainly DP = 2-4) also showed ability to increase the percentages of Bifidobacterium and the production of both butyrate and acetate.					
30113166	0	36	dep	Approach	180:187	arg1	Potential					0:8	Potential	0:8	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota	0:160	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	4	37	theme	=	1073:1073	arg1	DP					1070:1071	DP	1070:1071	mainly DP = 2-4	1063:1077	XOS2 (mainly DP = 2-4) also showed ability to increase the percentages of Bifidobacterium and the production of both butyrate and acetate.					
30113166	2	38	theme	total	510:514	arg1	numbers					526:532	the total bacterial numbers	506:532	the total bacterial numbers	506:532	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	0	39	theme	First	165:169	arg1	Approach					180:187	A First in Vitro Approach	163:187	A First in Vitro Approach	163:187	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	1	40	theme	different	393:401	arg1	doses					403:407	different doses	393:407	different doses on the elderly gut microbiota and its metabolic activity	393:464	Fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were employed as substrates for in vitro fermentations to assess their capacity to counteract the effects caused by three antibiotics (ABs) at different doses on the elderly gut microbiota and its metabolic activity.					
30113166	2	41	theme	%	672:672	arg1	XOS1					701:704	XOS1	701:704	XOS1	701:704	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	41	theme	%	672:672	arg1	%					680:680	11.4% to 3.2%	668:680	11.4% to 3.2% in the presence of XOS1	668:704	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	3	42	theme	constant	1017:1024	arg1	Bacteroides					998:1008	Bacteroides	998:1008	Bacteroides almost constant	998:1024	FOS were able to counteract these effects by increasing the butyrate production and the number of Lactobacillus, while maintaining the number of Bacteroides almost constant and decreasing the clostridia.					
30113166	0	43	dep	in	171:172	arg1	Vitro					174:178	Vitro	174:178	Vitro	174:178	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	3	44	theme	butyrate	913:920	arg1	production					922:931	the butyrate production	909:931	the butyrate production	909:931	FOS were able to counteract these effects by increasing the butyrate production and the number of Lactobacillus, while maintaining the number of Bacteroides almost constant and decreasing the clostridia.					
30113166	0	45	theme	in	171:172	arg1	Approach					180:187	A First in Vitro Approach	163:187	A First in Vitro Approach	163:187	Potential of Fructooligosaccharides and Xylooligosaccharides as Substrates To Counteract the Undesirable Effects of Several Antibiotics on Elder Fecal Microbiota: A First in Vitro Approach.					
30113166	2	46	theme	butyrate	723:730	arg1	production					732:741	the butyrate production	719:741	the butyrate production	719:741	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	1	47	theme	elderly	416:422	arg1	microbiota					428:437	the elderly gut microbiota	412:437	the elderly gut microbiota	412:437	Fructooligosaccharides (FOS) and xylooligosaccharides (XOS) were employed as substrates for in vitro fermentations to assess their capacity to counteract the effects caused by three antibiotics (ABs) at different doses on the elderly gut microbiota and its metabolic activity.					
30113166	2	48	dep	decreased	652:660	arg1	XOS1					701:704	XOS1	701:704	XOS1	701:704	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	48	dep	decreased	652:660	arg1	%					680:680	11.4% to 3.2%	668:680	11.4% to 3.2% in the presence of XOS1	668:704	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
30113166	2	49	theme	Bacteroides	770:780	arg1	population					756:765	the population	752:765	the population of Bacteroides	752:780	The AB type and dose scarcely affected the total bacterial numbers and the microbiota composition after 24 h. However, in the presence of ABs, the relative percentages of Lactobacillus decreased (from 11.4% to 3.2% in the presence of XOS1), as well as the butyrate production, whereas the population of Bacteroides increased significantly in the presence of XOS1 (from 27.5% to 55.7%).					
31163395	0	0	from	determination	17:29	arg1	serum					52:56	pig serum	48:56	pig serum based on ultrathin CuS	48:79	Highly sensitive determination of L-tyrosine in pig serum based on ultrathin CuS nanosheets composite electrode.					
31163395	4	1	theme	NS-CS/F-MWCNTs/GCE	881:898	arg1	electrode					864:872	the modified electrode	851:872	the modified electrode of CuS NS-CS/F-MWCNTs/GCE	851:898	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	2	2	theme	glassy	357:362	arg1	GCE					382:384	GCE	382:384	GCE	382:384	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	2	2	theme	glassy	357:362	arg1	electrode					371:379	glassy carbon electrode	357:379	glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs)	357:469	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	4	3	theme	cyclic	769:774	arg1	CV					789:790	CV	789:790	CV	789:790	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	4	3	theme	cyclic	769:774	arg1	voltammetry					776:786	cyclic voltammetry	769:786	cyclic voltammetry (CV)	769:791	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	0	4	theme	ultrathin	67:75	arg1	CuS					77:79	ultrathin CuS	67:79	ultrathin CuS	67:79	Highly sensitive determination of L-tyrosine in pig serum based on ultrathin CuS nanosheets composite electrode.					
31163395	3	5	theme	4.50 ± 0.58 nm	647:660	arg1	thickness					634:642	thickness	634:642	thickness	634:642	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	3	5	theme	4.50 ± 0.58 nm	647:660	arg1	13.33 ± 0.67 nm					614:628	13.33 ± 0.67 nm	614:628	13.33 ± 0.67 nm	614:628	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	5	6	theme	linear	1051:1056	arg1	range					1067:1071	a wide linear response range	1044:1071	a wide linear response range for L-Tyr (0.08-1.0 μM)	1044:1095	Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08-1.0 μM) with a detection limit of 4.9 nM.					
31163395	8	7	theme	pig	1504:1506	arg1	serums					1508:1513	pig serums	1504:1513	pig serums with a recovery rate of 95.7%-102.6%	1504:1550	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	8	8	theme	good	1581:1584	arg1	agreement					1586:1594	good agreement	1581:1594	good agreement with that of HPLC	1581:1612	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	0	9	theme	composite	92:100	arg1	electrode					102:110	composite electrode	92:110	composite electrode	92:110	Highly sensitive determination of L-tyrosine in pig serum based on ultrathin CuS nanosheets composite electrode.					
31163395	5	10	theme	4.9 nM	1123:1128	arg1	limit					1114:1118	a detection limit	1102:1118	a detection limit of 4.9 nM	1102:1128	Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08-1.0 μM) with a detection limit of 4.9 nM.					
31163395	3	11	theme	electron	524:531	arg1	TEM					545:547	TEM	545:547	TEM	545:547	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	3	11	theme	electron	524:531	arg1	microscopy					533:542	transmission electron microscopy	511:542	transmission electron microscopy (TEM)	511:548	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	5	12	theme	optimal	992:998	arg1	condition					1000:1008	the optimal condition	988:1008	the optimal condition	988:1008	Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08-1.0 μM) with a detection limit of 4.9 nM.					
31163395	8	13	from	determination	1478:1490	arg1	serums					1508:1513	pig serums	1504:1513	pig serums with a recovery rate of 95.7%-102.6%	1504:1550	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	4	14	theme	modified	855:862	arg1	electrode					864:872	the modified electrode	851:872	the modified electrode of CuS NS-CS/F-MWCNTs/GCE	851:898	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	4	15	theme	electrochemical	667:681	arg1	characteristics					683:697	The electrochemical characteristics	663:697	The electrochemical characteristics of different nanocomposite modified electrodes	663:744	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	6	16	from	coexisted	1161:1169	arg1	interferences					1142:1154	No obvious interferences	1131:1154	No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids	1131:1227	No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids could be observed, indicating its relatively good selectivity.					
31163395	3	17	theme	Scanning	472:479	arg1	SEM					502:504	SEM	502:504	SEM	502:504	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	3	17	theme	Scanning	472:479	arg1	microscopy					490:499	Scanning electron microscopy	472:499	Scanning electron microscopy (SEM)	472:505	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	4	18	contain	possessed	900:908	arg2	activity					932:939	good electrocatalytic activity	910:939	good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr)	910:979	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	4	18	contain	possessed	900:908	arg1	electrode					864:872	the modified electrode	851:872	the modified electrode of CuS NS-CS/F-MWCNTs/GCE	851:898	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	8	19	with	serums	1508:1513	arg1	rate					1531:1534	a recovery rate	1520:1534	a recovery rate of 95.7%-102.6%	1520:1550	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	0	20	theme	pig	48:50	arg1	serum					52:56	pig serum	48:56	pig serum based on ultrathin CuS	48:79	Highly sensitive determination of L-tyrosine in pig serum based on ultrathin CuS nanosheets composite electrode.					
31163395	2	21	theme	multi-walled	430:441	arg1	nanotubes					450:458	acidified multi-walled carbon nanotubes	420:458	acidified multi-walled carbon nanotubes (F-MWCNTs)	420:469	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	2	21	theme	multi-walled	430:441	arg1	F-MWCNTs					461:468	F-MWCNTs	461:468	F-MWCNTs	461:468	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	0	22	theme	sensitive	7:15	arg1	determination					17:29	Highly sensitive determination	0:29	Highly sensitive determination of L-tyrosine in pig serum based on ultrathin CuS	0:79	Highly sensitive determination of L-tyrosine in pig serum based on ultrathin CuS nanosheets composite electrode.					
31163395	3	23	with	hexagon	586:592	arg1	length					604:609	side length	599:609	side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm	599:660	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	4	24	theme	electrodes	735:744	arg1	characteristics					683:697	The electrochemical characteristics	663:697	The electrochemical characteristics of different nanocomposite modified electrodes	663:744	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	1	25	theme	electrical	189:198	arg1	conductivity					200:211	electrical conductivity	189:211	electrical conductivity	189:211	Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity.					
31163395	2	26	theme	acidified	420:428	arg1	nanotubes					450:458	acidified multi-walled carbon nanotubes	420:458	acidified multi-walled carbon nanotubes (F-MWCNTs)	420:469	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	2	26	theme	acidified	420:428	arg1	F-MWCNTs					461:468	F-MWCNTs	461:468	F-MWCNTs	461:468	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	8	27	theme	95.7	1539:1542	arg1	%					1543:1543	%	1543:1543	%	1543:1543	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	4	28	theme	modified	726:733	arg1	electrodes					735:744	different nanocomposite modified electrodes	702:744	different nanocomposite modified electrodes	702:744	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	6	29	theme	good	1274:1277	arg1	selectivity					1279:1289	its relatively good selectivity	1259:1289	its relatively good selectivity	1259:1289	No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids could be observed, indicating its relatively good selectivity.					
31163395	2	30	theme	copper	268:273	arg1	CuS					295:297	CuS	295:297	CuS	295:297	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	2	30	theme	copper	268:273	arg1	nanosheets					283:292	ultrathin copper sulfide nanosheets	258:292	ultrathin copper sulfide nanosheets (CuS NS)	258:301	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	4	31	theme	electrocatalytic	915:930	arg1	activity					932:939	good electrocatalytic activity	910:939	good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr)	910:979	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	6	32	theme	other	1211:1215	arg1	acids					1223:1227	other amino acids	1211:1227	other amino acids	1211:1227	No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids could be observed, indicating its relatively good selectivity.					
31163395	8	33	theme	commercial	1382:1391	arg1	method					1415:1420	a commercial instrument analytical method	1380:1420	a commercial instrument analytical method	1380:1420	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	8	33	theme	commercial	1382:1391	arg1	HPLC					1423:1426	HPLC	1423:1426	HPLC	1423:1426	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	8	34	theme	analytical	1404:1413	arg1	method					1415:1420	a commercial instrument analytical method	1380:1420	a commercial instrument analytical method	1380:1420	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	8	34	theme	analytical	1404:1413	arg1	HPLC					1423:1426	HPLC	1423:1426	HPLC	1423:1426	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	6	35	theme	obvious	1134:1140	arg1	interferences					1142:1154	No obvious interferences	1131:1154	No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids	1131:1227	No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids could be observed, indicating its relatively good selectivity.					
31163395	8	36	theme	-102.6	1544:1549	arg1	%					1543:1543	%	1543:1543	%	1543:1543	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	4	37	theme	good	910:913	arg1	activity					932:939	good electrocatalytic activity	910:939	good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr)	910:979	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	8	38	theme	instrument	1393:1402	arg1	method					1415:1420	a commercial instrument analytical method	1380:1420	a commercial instrument analytical method	1380:1420	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	8	38	theme	instrument	1393:1402	arg1	HPLC					1423:1426	HPLC	1423:1426	HPLC	1423:1426	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	1	39	theme	electrocatalytic	217:232	arg1	activity					234:241	electrocatalytic activity	217:241	electrocatalytic activity	217:241	Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity.					
31163395	3	40	theme	electron	481:488	arg1	SEM					502:504	SEM	502:504	SEM	502:504	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	3	40	theme	electron	481:488	arg1	microscopy					490:499	Scanning electron microscopy	472:499	Scanning electron microscopy (SEM)	472:505	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	4	41	theme	pulse	810:814	arg1	DPV					829:831	DPV	829:831	DPV	829:831	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	4	41	theme	pulse	810:814	arg1	voltammetry					816:826	differential pulse voltammetry	797:826	differential pulse voltammetry (DPV)	797:832	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	7	42	theme	good	1315:1318	arg1	repeatability					1320:1332	good repeatability	1315:1332	good repeatability	1315:1332	The electrode also had good repeatability, reproducibility and stability.					
31163395	0	43	theme	L-tyrosine	34:43	arg1	determination					17:29	Highly sensitive determination	0:29	Highly sensitive determination of L-tyrosine in pig serum based on ultrathin CuS	0:79	Highly sensitive determination of L-tyrosine in pig serum based on ultrathin CuS nanosheets composite electrode.					
31163395	4	44	theme	differential	797:808	arg1	DPV					829:831	DPV	829:831	DPV	829:831	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	4	44	theme	differential	797:808	arg1	voltammetry					816:826	differential pulse voltammetry	797:826	differential pulse voltammetry (DPV)	797:832	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	4	45	theme	L-tyrosine	962:971	arg1	oxidation					949:957	oxidation	949:957	oxidation of L-tyrosine (L-Tyr)	949:979	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	8	46	theme	test	1561:1564	arg1	results					1566:1572	its test results	1557:1572	its test results	1557:1572	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	4	47	theme	CuS	877:879	arg1	NS-CS/F-MWCNTs/GCE					881:898	CuS NS-CS/F-MWCNTs/GCE	877:898	CuS NS-CS/F-MWCNTs/GCE	877:898	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	3	48	theme	side	599:602	arg1	length					604:609	side length	599:609	side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm	599:660	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	6	49	theme	amino	1217:1221	arg1	acids					1223:1227	other amino acids	1211:1227	other amino acids	1211:1227	No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids could be observed, indicating its relatively good selectivity.					
31163395	1	50	theme	Nanometer-sized	113:127	arg1	sulfide					136:142	Nanometer-sized copper sulfide	113:142	Nanometer-sized copper sulfide	113:142	Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity.					
31163395	2	51	theme	carbon	443:448	arg1	nanotubes					450:458	acidified multi-walled carbon nanotubes	420:458	acidified multi-walled carbon nanotubes (F-MWCNTs)	420:469	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	2	51	theme	carbon	443:448	arg1	F-MWCNTs					461:468	F-MWCNTs	461:468	F-MWCNTs	461:468	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	5	52	theme	detection	1104:1112	arg1	limit					1114:1118	a detection limit	1102:1118	a detection limit of 4.9 nM	1102:1128	Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08-1.0 μM) with a detection limit of 4.9 nM.					
31163395	3	53	theme	thickness	634:642	arg1	length					604:609	side length	599:609	side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm	599:660	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	5	54	theme	wide	1046:1049	arg1	range					1067:1071	a wide linear response range	1044:1071	a wide linear response range for L-Tyr (0.08-1.0 μM)	1044:1095	Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08-1.0 μM) with a detection limit of 4.9 nM.					
31163395	1	55	theme	copper	129:134	arg1	sulfide					136:142	Nanometer-sized copper sulfide	113:142	Nanometer-sized copper sulfide	113:142	Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity.					
31163395	3	56	theme	NS	579:580	arg1	shape					566:570	the shape	562:570	the shape of CuS NS	562:580	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	3	56	theme	NS	579:580	arg1	hexagon					586:592	hexagon	586:592	hexagon	586:592	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	1	57	theme	remarkable	148:157	arg1	properties					159:168	remarkable properties	148:168	remarkable properties such as metal	148:182	Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity.					
31163395	1	57	theme	remarkable	148:157	arg1	metal					178:182	metal	178:182	metal	178:182	Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity.					
31163395	5	58	theme	modified	1015:1022	arg1	electrode					1024:1032	the modified electrode	1011:1032	the modified electrode	1011:1032	Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08-1.0 μM) with a detection limit of 4.9 nM.					
31163395	4	59	theme	nanocomposite	712:724	arg1	electrodes					735:744	different nanocomposite modified electrodes	702:744	different nanocomposite modified electrodes	702:744	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	8	60	with	agreement	1586:1594	arg1	that					1601:1604	that	1601:1604	that	1601:1604	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	3	61	theme	transmission	511:522	arg1	TEM					545:547	TEM	545:547	TEM	545:547	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	3	61	theme	transmission	511:522	arg1	microscopy					533:542	transmission electron microscopy	511:542	transmission electron microscopy (TEM)	511:548	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	3	62	theme	13.33 ± 0.67 nm	614:628	arg1	length					604:609	side length	599:609	side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm	599:660	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	2	63	theme	sulfide	275:281	arg1	CuS					295:297	CuS	295:297	CuS	295:297	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	2	63	theme	sulfide	275:281	arg1	nanosheets					283:292	ultrathin copper sulfide nanosheets	258:292	ultrathin copper sulfide nanosheets (CuS NS)	258:301	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	8	64	theme	L-Tyr	1495:1499	arg1	determination					1478:1490	the determination	1474:1490	the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%	1474:1550	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	4	65	theme	different	702:710	arg1	electrodes					735:744	different nanocomposite modified electrodes	702:744	different nanocomposite modified electrodes	702:744	The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr).					
31163395	7	66	contain	had	1311:1313	arg1	electrode					1296:1304	The electrode	1292:1304	The electrode	1292:1304	The electrode also had good repeatability, reproducibility and stability.					
31163395	7	66	contain	had	1311:1313	arg2	reproducibility					1335:1349	reproducibility	1335:1349	reproducibility	1335:1349	The electrode also had good repeatability, reproducibility and stability.					
31163395	7	66	contain	had	1311:1313	arg2	repeatability					1320:1332	good repeatability	1315:1332	good repeatability	1315:1332	The electrode also had good repeatability, reproducibility and stability.					
31163395	7	66	contain	had	1311:1313	arg2	stability					1355:1363	stability	1355:1363	stability	1355:1363	The electrode also had good repeatability, reproducibility and stability.					
31163395	5	67	theme	response	1058:1065	arg1	range					1067:1071	a wide linear response range	1044:1071	a wide linear response range for L-Tyr (0.08-1.0 μM)	1044:1095	Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08-1.0 μM) with a detection limit of 4.9 nM.					
31163395	3	68	theme	CuS	575:577	arg1	NS					579:580	CuS NS	575:580	CuS NS	575:580	Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm.					
31163395	8	69	theme	recovery	1522:1529	arg1	rate					1531:1534	a recovery rate	1520:1534	a recovery rate of 95.7%-102.6%	1520:1550	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	2	70	theme	electrode	371:379	arg1	surface					346:352	surface	346:352	surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs)	346:469	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	8	71	theme	application	1637:1647	arg1	value					1649:1653	its promising application value	1623:1653	its promising application value	1623:1653	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	1	72	contain	has	144:146	arg1	sulfide					136:142	Nanometer-sized copper sulfide	113:142	Nanometer-sized copper sulfide	113:142	Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity.					
31163395	1	72	contain	has	144:146	arg2	metal					178:182	metal	178:182	metal	178:182	Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity.					
31163395	1	72	contain	has	144:146	arg2	properties					159:168	remarkable properties	148:168	remarkable properties such as metal	148:182	Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity.					
31163395	2	73	theme	ultrathin	258:266	arg1	CuS					295:297	CuS	295:297	CuS	295:297	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	2	73	theme	ultrathin	258:266	arg1	nanosheets					283:292	ultrathin copper sulfide nanosheets	258:292	ultrathin copper sulfide nanosheets (CuS NS)	258:301	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	2	74	theme	carbon	364:369	arg1	GCE					382:384	GCE	382:384	GCE	382:384	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	2	74	theme	carbon	364:369	arg1	electrode					371:379	glassy carbon electrode	357:379	glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs)	357:469	In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs).					
31163395	8	75	theme	promising	1627:1635	arg1	value					1649:1653	its promising application value	1623:1653	its promising application value	1623:1653	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31163395	8	76	theme	%	1550:1550	arg1	rate					1531:1534	a recovery rate	1520:1534	a recovery rate of 95.7%-102.6%	1520:1550	Compared with a commercial instrument analytical method, HPLC, the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%-102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.					
31004632	2	0	theme	respiration	501:511	arg1	rate					513:516	respiration rate	501:516	respiration rate	501:516	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	8	1	theme	different	1337:1345	arg1	points					1347:1352	different points	1337:1352	different points of PCF11	1337:1361	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	4	2	theme	acids	767:771	arg1	loss					750:753	loss	750:753	loss of phenolic acids	750:771	Edible coatings also protected loss of phenolic acids like gallic and p-coumaric acid in tomato.					
31004632	4	3	theme	gallic	778:783	arg1	acid					800:803	gallic and p-coumaric acid	778:803	gallic and p-coumaric acid in tomato	778:813	Edible coatings also protected loss of phenolic acids like gallic and p-coumaric acid in tomato.					
31004632	6	4	theme	little	1034:1039	arg1	hydrophobicity					1041:1054	little hydrophobicity	1034:1054	little hydrophobicity	1034:1054	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	7	5	theme	component	1179:1187	arg1	analysis					1189:1196	Principal component analysis	1169:1196	Principal component analysis	1169:1196	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	3	6	theme	fructose	599:606	arg1	glucose					587:593	glucose	587:593	glucose	587:593	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	3	6	theme	fructose	599:606	arg1	amounts					576:582	Lower amounts	570:582	Lower amounts of glucose and fructose quantified through HPLC	570:630	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	3	6	theme	fructose	599:606	arg1	fructose					599:606	fructose	599:606	fructose	599:606	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	6	7	theme	size	1131:1134	arg1	/WVP					1142:1145	z-average particle size (d.nm)/WVP	1112:1145	z-average particle size (d.nm)/WVP of coating solutions	1112:1166	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	7	8	theme	shelf	1292:1296	arg1	study					1303:1307	shelf life study	1292:1307	shelf life study	1292:1307	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	3	9	theme	cell	649:652	arg1	components					659:668	cell wall components	649:668	lesser cell wall components degradation	642:680	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	6	10	theme	z-average	1112:1120	arg1	size					1131:1134	z-average particle size	1112:1134	z-average particle size (d.nm)/WVP of coating solutions	1112:1166	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	6	10	theme	z-average	1112:1120	arg1	d.nm					1137:1140	d.nm	1137:1140	d.nm	1137:1140	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	6	11	theme	coatings	1083:1090	arg1	uniformity					1019:1028	uniformity	1019:1028	uniformity	1019:1028	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	6	11	theme	coatings	1083:1090	arg1	hydrophobicity					1041:1054	little hydrophobicity	1034:1054	little hydrophobicity	1034:1054	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	6	12	dep	uniformity	1019:1028	arg1	the					1015:1017	the	1015:1017	the	1015:1017	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	1	13	theme	based	308:312	arg1	coatings					314:321	based coatings	308:321	based coatings	308:321	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
31004632	1	14	theme	pink-red	233:240	arg1	stage					242:246	pink-red stage	233:246	pink-red stage	233:246	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
31004632	4	15	theme	p-coumaric	789:798	arg1	acid					800:803	gallic and p-coumaric acid	778:803	gallic and p-coumaric acid in tomato	778:813	Edible coatings also protected loss of phenolic acids like gallic and p-coumaric acid in tomato.					
31004632	0	16	from	flour	55:59	arg1	coatings					17:24	Composite edible coatings	0:24	Composite edible coatings from commercial pectin, corn flour and beetroot powder	0:79	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	6	17	theme	solutions	1158:1166	arg1	/WVP					1142:1145	z-average particle size (d.nm)/WVP	1112:1145	z-average particle size (d.nm)/WVP of coating solutions	1112:1166	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	2	18	from	firmness	530:537	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	3	19	theme	components	659:668	arg1	degradation					670:680	lesser cell wall components degradation	642:680	lesser cell wall components degradation	642:680	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	0	20	theme	sensory	140:146	arg1	liking					148:153	sensory liking	140:153	sensory liking of tomatoes	140:165	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	5	21	theme	scores	920:925	arg1	retention					881:889	higher retention	874:889	higher retention of gloss and overall flavour scores	874:925	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	6	22	theme	flour-based	1071:1081	arg1	coatings					1083:1090	pectin-corn flour-based coatings	1059:1090	pectin-corn flour-based coatings	1059:1090	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	7	23	theme	several	1234:1240	arg1	treatments					1242:1251	several treatments	1234:1251	several treatments	1234:1251	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	6	24	theme	coating	1150:1156	arg1	solutions					1158:1166	coating solutions	1150:1166	coating solutions	1150:1166	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	2	25	from	activity	560:567	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	26	theme	tomato	417:422	arg1	life					387:390	shelf life	381:390	shelf life	381:390	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	26	theme	tomato	417:422	arg1	retention					404:412	quality retention	396:412	quality retention of tomato in terms of physiological weight loss (PWL)	396:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	5	27	dep	coatings	826:833	arg1	PCF11B					848:853	PCF11B	848:853	PCF11B in tomatoes	848:865	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	5	27	dep	coatings	826:833	arg1	coatings					826:833	All three coatings P, PCF11 and PCF11B in tomatoes	816:865	All three coatings P, PCF11 and PCF11B in tomatoes	816:865	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	5	27	dep	coatings	826:833	arg1	PCF11					838:842	PCF11	838:842	PCF11	838:842	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	5	27	dep	coatings	826:833	arg1	P					835:835	P	835:835	P	835:835	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	0	28	theme	beetroot	65:72	arg1	powder					74:79	beetroot powder	65:79	beetroot powder	65:79	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	2	29	theme	loss	457:460	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	7	30	theme	treatments	1242:1251	arg1	correlation					1219:1229	significant correlation	1207:1229	significant correlation of several treatments	1207:1251	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	7	30	theme	treatments	1242:1251	arg1	effects					1265:1271	storage effects	1257:1271	storage effects on tomatoes	1257:1283	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	7	31	from	correlation	1219:1229	arg1	tomatoes					1276:1283	tomatoes	1276:1283	tomatoes	1276:1283	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	2	32	theme	physiological	436:448	arg1	PWL					463:465	PWL	463:465	PWL	463:465	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	32	theme	physiological	436:448	arg1	loss					457:460	physiological weight loss	436:460	physiological weight loss (PWL)	436:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	33	from	life	387:390	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	34	theme	PCF11	324:328	arg1	coating					330:336	PCF11 coating	324:336	PCF11 coating	324:336	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	7	35	theme	significant	1207:1217	arg1	correlation					1219:1229	significant correlation	1207:1229	significant correlation of several treatments	1207:1251	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	2	36	theme	shelf	381:385	arg1	life					387:390	shelf life	381:390	shelf life	381:390	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	8	37	theme	storage	1414:1420	arg1	intervals					1422:1430	subsequent storage intervals	1403:1430	subsequent storage intervals	1403:1430	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	0	38	theme	Composite	0:8	arg1	coatings					17:24	Composite edible coatings	0:24	Composite edible coatings from commercial pectin, corn flour and beetroot powder	0:79	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	2	39	theme	antioxidant	548:558	arg1	activity					560:567	DPPH antioxidant activity	543:567	DPPH antioxidant activity	543:567	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	7	40	from	effects	1265:1271	arg1	tomatoes					1276:1283	tomatoes	1276:1283	tomatoes	1276:1283	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	7	41	theme	Principal	1169:1177	arg1	analysis					1189:1196	Principal component analysis	1169:1196	Principal component analysis	1169:1196	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	2	42	theme	retention	404:412	arg1	firmness					530:537	firmness	530:537	firmness	530:537	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	42	theme	retention	404:412	arg1	decay					469:473	decay	469:473	decay per cent	469:482	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	42	theme	retention	404:412	arg1	rate					513:516	respiration rate	501:516	respiration rate	501:516	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	42	theme	retention	404:412	arg1	angle					523:527	hue angle	519:527	hue angle	519:527	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	42	theme	retention	404:412	arg1	activity					560:567	DPPH antioxidant activity	543:567	DPPH antioxidant activity	543:567	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	42	theme	retention	404:412	arg1	index					494:498	ripening index	485:498	ripening index	485:498	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	42	theme	retention	404:412	arg1	enhancement					366:376	significant enhancement	354:376	significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL)	354:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	0	43	theme	commercial	31:40	arg1	pectin					42:47	commercial pectin	31:47	commercial pectin	31:47	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	2	44	from	rate	513:516	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	5	45	theme	flavour	912:918	arg1	scores					920:925	overall flavour scores	904:925	overall flavour scores	904:925	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	8	46	theme	tomatoes	1467:1474	arg1	quality					1456:1462	quality	1456:1462	quality of tomatoes	1456:1474	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	3	47	theme	Lower	570:574	arg1	glucose					587:593	glucose	587:593	glucose	587:593	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	3	47	theme	Lower	570:574	arg1	amounts					576:582	Lower amounts	570:582	Lower amounts of glucose and fructose quantified through HPLC	570:630	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	3	47	theme	Lower	570:574	arg1	fructose					599:606	fructose	599:606	fructose	599:606	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	5	48	from	PCF11	838:842	arg1	tomatoes					858:865	tomatoes	858:865	tomatoes	858:865	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	1	49	theme	shelf	207:211	arg1	life					213:216	the shelf life	203:216	the shelf life of tomatoes	203:228	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
31004632	5	50	theme	higher	874:879	arg1	retention					881:889	higher retention	874:889	higher retention of gloss and overall flavour scores	874:925	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	8	51	theme	least	1314:1318	arg1	separation					1320:1329	The least separation	1310:1329	The least separation among different points of PCF11 in score plot	1310:1375	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	5	52	from	P	835:835	arg1	tomatoes					858:865	tomatoes	858:865	tomatoes	858:865	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	8	53	theme	quality	1456:1462	arg1	retention					1443:1451	retention	1443:1451	retention of quality of tomatoes during storage	1443:1489	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	4	54	theme	phenolic	758:765	arg1	acids					767:771	phenolic acids	758:771	phenolic acids	758:771	Edible coatings also protected loss of phenolic acids like gallic and p-coumaric acid in tomato.					
31004632	2	55	from	decay	469:473	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	5	56	from	PCF11B	848:853	arg1	tomatoes					858:865	tomatoes	858:865	tomatoes	858:865	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	2	57	theme	ripening	485:492	arg1	index					494:498	ripening index	485:498	ripening index	485:498	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	6	58	from	shrinkage	949:957	arg1	tomatoes					968:975	tomatoes	968:975	tomatoes coated with PCF11 on 30th day	968:1005	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	2	59	theme	hue	519:521	arg1	angle					523:527	hue angle	519:527	hue angle	519:527	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	8	60	from	separation	1320:1329	arg1	plot					1372:1375	score plot	1366:1375	score plot	1366:1375	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	6	61	theme	particle	1122:1129	arg1	size					1131:1134	z-average particle size	1112:1134	z-average particle size (d.nm)/WVP of coating solutions	1112:1166	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	6	61	theme	particle	1122:1129	arg1	d.nm					1137:1140	d.nm	1137:1140	d.nm	1137:1140	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	1	62	theme	present	172:178	arg1	study					180:184	The present study	168:184	The present study	168:184	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
31004632	7	63	theme	life	1298:1301	arg1	study					1303:1307	shelf life study	1292:1307	shelf life study	1292:1307	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	3	64	theme	wall	654:657	arg1	components					659:668	cell wall components	649:668	lesser cell wall components degradation	642:680	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	0	65	from	powder	74:79	arg1	coatings					17:24	Composite edible coatings	0:24	Composite edible coatings from commercial pectin, corn flour and beetroot powder	0:79	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	3	66	theme	lesser	642:647	arg1	degradation					670:680	lesser cell wall components degradation	642:680	lesser cell wall components degradation	642:680	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	2	67	from	terms	427:431	arg1	firmness					530:537	firmness	530:537	firmness	530:537	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	67	from	terms	427:431	arg1	decay					469:473	decay	469:473	decay per cent	469:482	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	67	from	terms	427:431	arg1	rate					513:516	respiration rate	501:516	respiration rate	501:516	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	67	from	terms	427:431	arg1	angle					523:527	hue angle	519:527	hue angle	519:527	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	67	from	terms	427:431	arg1	activity					560:567	DPPH antioxidant activity	543:567	DPPH antioxidant activity	543:567	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	67	from	terms	427:431	arg1	index					494:498	ripening index	485:498	ripening index	485:498	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	67	from	terms	427:431	arg1	enhancement					366:376	significant enhancement	354:376	significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL)	354:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	8	68	theme	least	1384:1388	arg1	deviation					1390:1398	least deviation	1384:1398	least deviation	1384:1398	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	8	69	theme	PCF11	1357:1361	arg1	points					1347:1352	different points	1337:1352	different points of PCF11	1337:1361	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	7	70	theme	storage	1257:1263	arg1	effects					1265:1271	storage effects	1257:1271	storage effects on tomatoes	1257:1283	Principal component analysis revealed significant correlation of several treatments and storage effects on tomatoes during shelf life study.					
31004632	0	71	theme	tomatoes	158:165	arg1	liking					148:153	sensory liking	140:153	sensory liking of tomatoes	140:165	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	4	72	from	acid	800:803	arg1	tomato					808:813	tomato	808:813	tomato	808:813	Edible coatings also protected loss of phenolic acids like gallic and p-coumaric acid in tomato.					
31004632	8	73	theme	score	1366:1370	arg1	plot					1372:1375	score plot	1366:1375	score plot	1366:1375	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	6	74	theme	pectin-corn	1059:1069	arg1	coatings					1083:1090	pectin-corn flour-based coatings	1059:1090	pectin-corn flour-based coatings	1059:1090	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	2	75	from	enhancement	366:376	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	1	76	dep	pectin	256:261	arg1	coatings					314:321	based coatings	308:321	based coatings	308:321	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
31004632	2	77	theme	DPPH	543:546	arg1	activity					560:567	DPPH antioxidant activity	543:567	DPPH antioxidant activity	543:567	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	0	78	theme	post-harvest	90:101	arg1	decay					103:107	post-harvest decay	90:107	post-harvest decay	90:107	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	1	79	theme	beetroot	288:295	arg1	B					305:305	B	305:305	B	305:305	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
31004632	1	79	theme	beetroot	288:295	arg1	powder					297:302	beetroot powder	288:302	beetroot powder (B)	288:306	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
31004632	2	80	theme	weight	450:455	arg1	PWL					463:465	PWL	463:465	PWL	463:465	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	80	theme	weight	450:455	arg1	loss					457:460	physiological weight loss	436:460	physiological weight loss (PWL)	436:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	8	81	theme	subsequent	1403:1412	arg1	intervals					1422:1430	subsequent storage intervals	1403:1430	subsequent storage intervals	1403:1430	The least separation among different points of PCF11 in score plot showed least deviation at subsequent storage intervals, revealing retention of quality of tomatoes during storage.					
31004632	0	82	from	pectin	42:47	arg1	coatings					17:24	Composite edible coatings	0:24	Composite edible coatings from commercial pectin, corn flour and beetroot powder	0:79	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	0	83	theme	edible	10:15	arg1	coatings					17:24	Composite edible coatings	0:24	Composite edible coatings from commercial pectin, corn flour and beetroot powder	0:79	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	6	84	theme	minimum	941:947	arg1	shrinkage					949:957	the minimum shrinkage	937:957	the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day	937:1005	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	6	84	theme	minimum	941:947	arg1	%					962:962	18%	960:962	18%	960:962	Further, the minimum shrinkage (18%) in tomatoes coated with PCF11 on 30th day evinced the uniformity and little hydrophobicity of pectin-corn flour-based coatings as observed through z-average particle size (d.nm)/WVP of coating solutions.					
31004632	2	85	from	index	494:498	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	86	theme	significant	354:364	arg1	enhancement					366:376	significant enhancement	354:376	significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL)	354:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	5	87	theme	gloss	894:898	arg1	retention					881:889	higher retention	874:889	higher retention of gloss and overall flavour scores	874:925	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	2	88	theme	quality	396:402	arg1	retention					404:412	quality retention	396:412	quality retention of tomato in terms of physiological weight loss (PWL)	396:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	0	89	theme	corn	50:53	arg1	flour					55:59	corn flour	50:59	corn flour	50:59	Composite edible coatings from commercial pectin, corn flour and beetroot powder minimize post-harvest decay, reduces ripening and improves sensory liking of tomatoes.					
31004632	1	90	theme	corn	268:271	arg1	CF					280:281	CF	280:281	CF	280:281	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
31004632	1	90	theme	corn	268:271	arg1	flour					273:277	corn flour	268:277	corn flour (CF)	268:282	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
31004632	5	91	theme	overall	904:910	arg1	scores					920:925	overall flavour scores	904:925	overall flavour scores	904:925	All three coatings P, PCF11 and PCF11B in tomatoes showed higher retention of gloss and overall flavour scores.					
31004632	2	92	from	retention	404:412	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	93	theme	life	387:390	arg1	firmness					530:537	firmness	530:537	firmness	530:537	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	93	theme	life	387:390	arg1	decay					469:473	decay	469:473	decay per cent	469:482	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	93	theme	life	387:390	arg1	rate					513:516	respiration rate	501:516	respiration rate	501:516	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	93	theme	life	387:390	arg1	angle					523:527	hue angle	519:527	hue angle	519:527	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	93	theme	life	387:390	arg1	activity					560:567	DPPH antioxidant activity	543:567	DPPH antioxidant activity	543:567	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	93	theme	life	387:390	arg1	index					494:498	ripening index	485:498	ripening index	485:498	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	93	theme	life	387:390	arg1	enhancement					366:376	significant enhancement	354:376	significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL)	354:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	2	94	from	angle	523:527	arg1	terms					427:431	terms	427:431	terms of physiological weight loss (PWL)	427:466	PCF11 coating was resulted in significant enhancement of shelf life and quality retention of tomato in terms of physiological weight loss (PWL), decay per cent, ripening index, respiration rate, hue angle, firmness and DPPH antioxidant activity.					
31004632	4	95	theme	Edible	719:724	arg1	coatings					726:733	Edible coatings	719:733	Edible coatings	719:733	Edible coatings also protected loss of phenolic acids like gallic and p-coumaric acid in tomato.					
31004632	3	96	theme	glucose	587:593	arg1	glucose					587:593	glucose	587:593	glucose	587:593	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	3	96	theme	glucose	587:593	arg1	amounts					576:582	Lower amounts	570:582	Lower amounts of glucose and fructose quantified through HPLC	570:630	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	3	96	theme	glucose	587:593	arg1	fructose					599:606	fructose	599:606	fructose	599:606	Lower amounts of glucose and fructose quantified through HPLC indicated lesser cell wall components degradation in tomatoes coated with P and PCF11.					
31004632	1	97	theme	tomatoes	221:228	arg1	life					213:216	the shelf life	203:216	the shelf life of tomatoes	203:228	The present study aimed to enhance the shelf life of tomatoes at pink-red stage through pectin (P), corn flour (CF) and beetroot powder (B) based coatings.					
29440901	6	0	theme	P28	1091:1093	arg1	release					1080:1086	controlled release	1069:1086	controlled release of P28	1069:1093	The heparinized mSIS membrane was designed to improve the immobilization efficacy and facilitate controlled release of P28.					
29440901	3	1	from	submucosa	519:527	arg1	matrix					486:491	The decellularized matrix	467:491	The decellularized matrix from the small intestinal submucosa (SIS)	467:533	The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response.					
29440901	2	2	theme	regeneration	351:362	arg1	technology					370:379	The guided bone regeneration (GBR) technology	335:379	The guided bone regeneration (GBR) technology	335:379	The guided bone regeneration (GBR) technology provides a promising strategy to cure osteoporotic defects using bioactive membranes.					
29440901	7	3	theme	cells	1238:1242	arg1	differentiation					1194:1208	osteogenic differentiation	1183:1208	osteogenic differentiation	1183:1208	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	3	theme	cells	1238:1242	arg1	viability					1168:1176	viability	1168:1176	viability	1168:1176	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	3	theme	cells	1238:1242	arg1	proliferation					1153:1165	proliferation	1153:1165	proliferation	1153:1165	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	4	from	rats	1264:1267	arg1	differentiation					1194:1208	osteogenic differentiation	1183:1208	osteogenic differentiation	1183:1208	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	4	from	rats	1264:1267	arg1	cells					1238:1242	bone marrow stromal stem cells	1213:1242	bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX)	1213:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	4	from	rats	1264:1267	arg1	viability					1168:1176	viability	1168:1176	viability	1168:1176	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	4	from	rats	1264:1267	arg1	proliferation					1153:1165	proliferation	1153:1165	proliferation	1153:1165	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	5	theme	P28	1096:1098	arg1	release					1100:1106	P28 release	1096:1106	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX)	1096:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	4	6	theme	mineralized	682:692	arg1	SIS					694:696	heparinized mineralized SIS	670:696	heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	670:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	6	theme	mineralized	682:692	arg1	membrane					792:799	a novel GBR membrane	780:799	a novel GBR membrane for guided osteoporotic bone regeneration	780:841	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	1	7	theme	great	204:208	arg1	challenge					210:218	a great challenge	202:218	a great challenge to clinicians	202:232	INTRODUCTION At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone.					
29440901	3	8	theme	natural	565:571	arg1	microenvironment					573:588	its natural microenvironment	561:588	its natural microenvironment	561:588	The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response.					
29440901	10	9	theme	cell	1630:1633	arg1	proliferation					1635:1647	cell proliferation	1630:1647	cell proliferation	1630:1647	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	9	10	theme	P28	1485:1487	arg1	release					1489:1495	P28 release	1485:1495	P28 release from mSIS-heparin-P28	1485:1517	RESULTS In vitro results showed that P28 release from mSIS-heparin-P28 occurred in a controlled manner, with a long-term release time of 40 days.					
29440901	12	11	theme	osteoporotic	2019:2030	arg1	defects					2032:2038	osteoporotic defects	2019:2038	osteoporotic defects	2019:2038	CONCLUSION The heparinized mSIS loaded with P28 may serve as a potential GBR membrane for repairing osteoporotic defects.					
29440901	10	12	theme	osteogenesis-related	1718:1737	arg1	genes					1739:1743	osteogenesis-related genes	1718:1743	osteogenesis-related genes	1718:1743	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	4	13	theme	protein	729:735	arg1	mSIS/P28					767:774	mSIS/P28	767:774	mSIS/P28	767:774	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	13	theme	protein	729:735	arg1	P28					762:764	bone morphogenetic protein 2 (BMP2)-related peptide P28	710:764	bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	710:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	14	dep	MATERIALS	620:628	arg1	developed					660:668	developed	660:668	developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration	660:841	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	3	15	theme	decellularized	471:484	arg1	matrix					486:491	The decellularized matrix	467:491	The decellularized matrix from the small intestinal submucosa (SIS)	467:533	The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response.					
29440901	7	16	theme	stem	1233:1236	arg1	cells					1238:1242	bone marrow stromal stem cells	1213:1242	bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX)	1213:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	17	from	effects	1138:1144	arg1	differentiation					1194:1208	osteogenic differentiation	1183:1208	osteogenic differentiation	1183:1208	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	17	from	effects	1138:1144	arg1	viability					1168:1176	viability	1168:1176	viability	1168:1176	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	17	from	effects	1138:1144	arg1	proliferation					1153:1165	proliferation	1153:1165	proliferation	1153:1165	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	17	from	effects	1138:1144	arg1	release					1100:1106	P28 release	1096:1106	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX)	1096:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	4	18	theme	bone	710:713	arg1	mSIS/P28					767:774	mSIS/P28	767:774	mSIS/P28	767:774	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	18	theme	bone	710:713	arg1	P28					762:764	bone morphogenetic protein 2 (BMP2)-related peptide P28	710:764	bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	710:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	0	19	theme	membrane	90:97	arg1	scaffolds					53:61	composite scaffolds	43:61	composite scaffolds of mineralized ECM/heparin membrane	43:97	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide.					
29440901	1	20	theme	osteoporotic	175:186	arg1	defects					188:194	osteoporotic defects	175:194	osteoporotic defects	175:194	INTRODUCTION At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone.					
29440901	6	21	theme	heparinized	976:986	arg1	membrane					993:1000	The heparinized mSIS membrane	972:1000	The heparinized mSIS membrane	972:1000	The heparinized mSIS membrane was designed to improve the immobilization efficacy and facilitate controlled release of P28.					
29440901	10	22	theme	genes	1739:1743	arg1	activity					1685:1692	alkaline phosphatase activity	1664:1692	alkaline phosphatase activity	1664:1692	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	10	22	theme	genes	1739:1743	arg1	expression					1704:1713	mRNA expression	1699:1713	mRNA expression of osteogenesis-related genes in rBMSCs-OVX	1699:1757	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	10	22	theme	genes	1739:1743	arg1	viability					1653:1661	viability	1653:1661	viability	1653:1661	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	10	22	theme	genes	1739:1743	arg1	proliferation					1635:1647	cell proliferation	1630:1647	cell proliferation	1630:1647	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	8	23	theme	calvarial	1344:1352	arg1	model					1361:1365	a critical-sized OVX calvarial defect model	1323:1365	a critical-sized OVX calvarial defect model	1323:1365	Furthermore, a critical-sized OVX calvarial defect model was used to assess the bone regeneration capability of mSIS-heparin-P28 in vivo.					
29440901	12	24	theme	potential	1982:1990	arg1	membrane					1996:2003	a potential GBR membrane	1980:2003	a potential GBR membrane for repairing osteoporotic defects	1980:2038	CONCLUSION The heparinized mSIS loaded with P28 may serve as a potential GBR membrane for repairing osteoporotic defects.					
29440901	12	24	theme	potential	1982:1990	arg1	mSIS					1946:1949	The heparinized mSIS	1930:1949	The heparinized mSIS loaded with P28	1930:1965	CONCLUSION The heparinized mSIS loaded with P28 may serve as a potential GBR membrane for repairing osteoporotic defects.					
29440901	2	25	theme	promising	392:400	arg1	strategy					402:409	a promising strategy	390:409	a promising strategy to cure osteoporotic defects using bioactive membranes	390:464	The guided bone regeneration (GBR) technology provides a promising strategy to cure osteoporotic defects using bioactive membranes.					
29440901	7	26	theme	osteogenic	1183:1192	arg1	differentiation					1194:1208	osteogenic differentiation	1183:1208	osteogenic differentiation	1183:1208	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	1	27	from	present	149:155	arg1	INTRODUCTION					133:144	INTRODUCTION	133:144	INTRODUCTION At present	133:155	INTRODUCTION At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone.					
29440901	9	28	theme	long-term	1559:1567	arg1	time					1577:1580	a long-term release time	1557:1580	a long-term release time of 40 days	1557:1591	RESULTS In vitro results showed that P28 release from mSIS-heparin-P28 occurred in a controlled manner, with a long-term release time of 40 days.					
29440901	6	29	theme	controlled	1069:1078	arg1	release					1080:1086	controlled release	1069:1086	controlled release of P28	1069:1093	The heparinized mSIS membrane was designed to improve the immobilization efficacy and facilitate controlled release of P28.					
29440901	0	30	theme	ECM/heparin	78:88	arg1	membrane					90:97	mineralized ECM/heparin membrane	66:97	mineralized ECM/heparin membrane	66:97	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide.					
29440901	0	31	theme	Guided	0:5	arg1	regeneration					25:36	Guided osteoporotic bone regeneration	0:36	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane	0:97	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide.					
29440901	1	32	theme	osteoporotic	283:294	arg1	bone					296:299	the osteoporotic bone as compared with the normal bone	279:332	the osteoporotic bone as compared with the normal bone	279:332	INTRODUCTION At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone.					
29440901	4	33	theme	peptide	754:760	arg1	mSIS/P28					767:774	mSIS/P28	767:774	mSIS/P28	767:774	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	33	theme	peptide	754:760	arg1	P28					762:764	bone morphogenetic protein 2 (BMP2)-related peptide P28	710:764	bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	710:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	8	34	theme	bone	1390:1393	arg1	capability					1408:1417	the bone regeneration capability	1386:1417	the bone regeneration capability of mSIS-heparin-P28	1386:1437	Furthermore, a critical-sized OVX calvarial defect model was used to assess the bone regeneration capability of mSIS-heparin-P28 in vivo.					
29440901	2	35	theme	osteoporotic	419:430	arg1	defects					432:438	osteoporotic defects	419:438	osteoporotic defects using bioactive membranes	419:464	The guided bone regeneration (GBR) technology provides a promising strategy to cure osteoporotic defects using bioactive membranes.					
29440901	0	36	theme	bone	20:23	arg1	regeneration					25:36	Guided osteoporotic bone regeneration	0:36	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane	0:97	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide.					
29440901	5	37	theme	P28	937:939	arg1	loading					941:947	P28 loading	937:947	P28 loading onto heparinized mSIS	937:969	These mSIS/P28 membranes were obtained through the mineralization of SIS (mSIS), followed by P28 loading onto heparinized mSIS.					
29440901	6	38	theme	immobilization	1030:1043	arg1	efficacy					1045:1052	the immobilization efficacy	1026:1052	the immobilization efficacy	1026:1052	The heparinized mSIS membrane was designed to improve the immobilization efficacy and facilitate controlled release of P28.					
29440901	9	39	dep	In	1456:1457	arg1	vitro					1459:1463	vitro	1459:1463	vitro	1459:1463	RESULTS In vitro results showed that P28 release from mSIS-heparin-P28 occurred in a controlled manner, with a long-term release time of 40 days.					
29440901	10	40	from	viability	1653:1661	arg1	rBMSCs-OVX					1748:1757	rBMSCs-OVX	1748:1757	rBMSCs-OVX	1748:1757	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	4	41	theme	guided	805:810	arg1	regeneration					830:841	guided osteoporotic bone regeneration	805:841	guided osteoporotic bone regeneration	805:841	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	0	42	theme	mineralized	66:76	arg1	membrane					90:97	mineralized ECM/heparin membrane	66:97	mineralized ECM/heparin membrane	66:97	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide.					
29440901	4	43	theme	osteoporotic	812:823	arg1	regeneration					830:841	guided osteoporotic bone regeneration	805:841	guided osteoporotic bone regeneration	805:841	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	3	44	theme	small	502:506	arg1	SIS					530:532	SIS	530:532	SIS	530:532	The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response.					
29440901	3	44	theme	small	502:506	arg1	submucosa					519:527	the small intestinal submucosa	498:527	the small intestinal submucosa (SIS)	498:533	The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response.					
29440901	1	45	theme	normal	322:327	arg1	bone					329:332	the normal bone	318:332	the normal bone	318:332	INTRODUCTION At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone.					
29440901	8	46	theme	mSIS-heparin-P28	1422:1437	arg1	capability					1408:1417	the bone regeneration capability	1386:1417	the bone regeneration capability of mSIS-heparin-P28	1386:1437	Furthermore, a critical-sized OVX calvarial defect model was used to assess the bone regeneration capability of mSIS-heparin-P28 in vivo.					
29440901	11	47	theme	bone	1900:1903	arg1	regeneration					1905:1916	osteoporotic bone regeneration	1887:1916	osteoporotic bone regeneration	1887:1916	In vivo experiments revealed that mSIS-heparin-P28 dramatically stimulated osteoporotic bone regeneration.					
29440901	10	48	from	activity	1685:1692	arg1	rBMSCs-OVX					1748:1757	rBMSCs-OVX	1748:1757	rBMSCs-OVX	1748:1757	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	12	49	dep	CONCLUSION	1919:1928	arg1	serve					1971:1975	serve	1971:1975	may serve as a potential GBR membrane for repairing osteoporotic defects	1967:2038	CONCLUSION The heparinized mSIS loaded with P28 may serve as a potential GBR membrane for repairing osteoporotic defects.					
29440901	10	50	theme	phosphatase	1673:1683	arg1	activity					1685:1692	alkaline phosphatase activity	1664:1692	alkaline phosphatase activity	1664:1692	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	4	51	theme	GBR	788:790	arg1	SIS					694:696	heparinized mineralized SIS	670:696	heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	670:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	51	theme	GBR	788:790	arg1	membrane					792:799	a novel GBR membrane	780:799	a novel GBR membrane for guided osteoporotic bone regeneration	780:841	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	1	52	theme	regeneration	254:265	arg1	capacity					267:274	the lower regeneration capacity	244:274	the lower regeneration capacity of the osteoporotic bone as compared with the normal bone	244:332	INTRODUCTION At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone.					
29440901	10	53	from	expression	1704:1713	arg1	rBMSCs-OVX					1748:1757	rBMSCs-OVX	1748:1757	rBMSCs-OVX	1748:1757	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	10	54	theme	extra	1783:1787	arg1	components					1800:1809	extra osteogenic components	1783:1809	extra osteogenic components	1783:1809	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	8	55	theme	critical-sized	1325:1338	arg1	model					1361:1365	a critical-sized OVX calvarial defect model	1323:1365	a critical-sized OVX calvarial defect model	1323:1365	Furthermore, a critical-sized OVX calvarial defect model was used to assess the bone regeneration capability of mSIS-heparin-P28 in vivo.					
29440901	2	56	theme	bone	346:349	arg1	technology					370:379	The guided bone regeneration (GBR) technology	335:379	The guided bone regeneration (GBR) technology	335:379	The guided bone regeneration (GBR) technology provides a promising strategy to cure osteoporotic defects using bioactive membranes.					
29440901	1	57	theme	defects	188:194	arg1	treatment					162:170	the treatment	158:170	the treatment of osteoporotic defects	158:194	INTRODUCTION At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone.					
29440901	10	58	from	proliferation	1635:1647	arg1	rBMSCs-OVX					1748:1757	rBMSCs-OVX	1748:1757	rBMSCs-OVX	1748:1757	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	4	59	theme	bone	825:828	arg1	regeneration					830:841	guided osteoporotic bone regeneration	805:841	guided osteoporotic bone regeneration	805:841	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	9	60	from	mSIS-heparin-P28	1502:1517	arg1	release					1489:1495	P28 release	1485:1495	P28 release from mSIS-heparin-P28	1485:1517	RESULTS In vitro results showed that P28 release from mSIS-heparin-P28 occurred in a controlled manner, with a long-term release time of 40 days.					
29440901	7	61	theme	marrow	1218:1223	arg1	cells					1238:1242	bone marrow stromal stem cells	1213:1242	bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX)	1213:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	9	62	theme	In	1456:1457	arg1	results					1465:1471	In vitro results	1456:1471	RESULTS In vitro results	1448:1471	RESULTS In vitro results showed that P28 release from mSIS-heparin-P28 occurred in a controlled manner, with a long-term release time of 40 days.					
29440901	4	63	theme	heparinized	670:680	arg1	SIS					694:696	heparinized mineralized SIS	670:696	heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	670:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	63	theme	heparinized	670:680	arg1	membrane					792:799	a novel GBR membrane	780:799	a novel GBR membrane for guided osteoporotic bone regeneration	780:841	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	9	64	dep	RESULTS	1448:1454	arg1	results					1465:1471	In vitro results	1456:1471	RESULTS In vitro results	1448:1471	RESULTS In vitro results showed that P28 release from mSIS-heparin-P28 occurred in a controlled manner, with a long-term release time of 40 days.					
29440901	10	65	theme	components	1800:1809	arg1	addition					1771:1778	the addition	1767:1778	the addition of extra osteogenic components	1767:1809	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	2	66	theme	guided	339:344	arg1	technology					370:379	The guided bone regeneration (GBR) technology	335:379	The guided bone regeneration (GBR) technology	335:379	The guided bone regeneration (GBR) technology provides a promising strategy to cure osteoporotic defects using bioactive membranes.					
29440901	3	67	theme	cell	605:608	arg1	response					610:617	cell response	605:617	cell response	605:617	The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response.					
29440901	7	68	from	differentiation	1194:1208	arg1	rBMSCs-OVX					1270:1279	rBMSCs-OVX	1270:1279	rBMSCs-OVX	1270:1279	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	68	from	differentiation	1194:1208	arg1	rats					1264:1267	ovariectomized rats	1249:1267	ovariectomized rats (rBMSCs-OVX)	1249:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	11	69	theme	osteoporotic	1887:1898	arg1	regeneration					1905:1916	osteoporotic bone regeneration	1887:1916	osteoporotic bone regeneration	1887:1916	In vivo experiments revealed that mSIS-heparin-P28 dramatically stimulated osteoporotic bone regeneration.					
29440901	5	70	theme	SIS	913:915	arg1	mineralization					895:908	the mineralization	891:908	the mineralization of SIS (mSIS)	891:922	These mSIS/P28 membranes were obtained through the mineralization of SIS (mSIS), followed by P28 loading onto heparinized mSIS.					
29440901	4	71	theme	morphogenetic	715:727	arg1	mSIS/P28					767:774	mSIS/P28	767:774	mSIS/P28	767:774	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	71	theme	morphogenetic	715:727	arg1	P28					762:764	bone morphogenetic protein 2 (BMP2)-related peptide P28	710:764	bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	710:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	7	72	theme	stromal	1225:1231	arg1	cells					1238:1242	bone marrow stromal stem cells	1213:1242	bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX)	1213:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	11	73	dep	In	1812:1813	arg1	vivo					1815:1818	vivo	1815:1818	vivo	1815:1818	In vivo experiments revealed that mSIS-heparin-P28 dramatically stimulated osteoporotic bone regeneration.					
29440901	7	74	theme	bone	1213:1216	arg1	marrow					1218:1223	bone marrow	1213:1223	bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX)	1213:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	9	75	theme	controlled	1533:1542	arg1	manner					1544:1549	a controlled manner	1531:1549	a controlled manner	1531:1549	RESULTS In vitro results showed that P28 release from mSIS-heparin-P28 occurred in a controlled manner, with a long-term release time of 40 days.					
29440901	6	76	theme	mSIS	988:991	arg1	membrane					993:1000	The heparinized mSIS membrane	972:1000	The heparinized mSIS membrane	972:1000	The heparinized mSIS membrane was designed to improve the immobilization efficacy and facilitate controlled release of P28.					
29440901	11	77	theme	In	1812:1813	arg1	experiments					1820:1830	In vivo experiments	1812:1830	In vivo experiments	1812:1830	In vivo experiments revealed that mSIS-heparin-P28 dramatically stimulated osteoporotic bone regeneration.					
29440901	0	78	theme	BMP2-related	111:122	arg1	peptide					124:130	BMP2-related peptide	111:130	BMP2-related peptide	111:130	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide.					
29440901	12	79	theme	GBR	1992:1994	arg1	membrane					1996:2003	a potential GBR membrane	1980:2003	a potential GBR membrane for repairing osteoporotic defects	1980:2038	CONCLUSION The heparinized mSIS loaded with P28 may serve as a potential GBR membrane for repairing osteoporotic defects.					
29440901	12	79	theme	GBR	1992:1994	arg1	mSIS					1946:1949	The heparinized mSIS	1930:1949	The heparinized mSIS loaded with P28	1930:1965	CONCLUSION The heparinized mSIS loaded with P28 may serve as a potential GBR membrane for repairing osteoporotic defects.					
29440901	10	80	theme	osteogenic	1789:1798	arg1	components					1800:1809	extra osteogenic components	1783:1809	extra osteogenic components	1783:1809	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	7	81	from	proliferation	1153:1165	arg1	rBMSCs-OVX					1270:1279	rBMSCs-OVX	1270:1279	rBMSCs-OVX	1270:1279	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	81	from	proliferation	1153:1165	arg1	rats					1264:1267	ovariectomized rats	1249:1267	ovariectomized rats (rBMSCs-OVX)	1249:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	8	82	theme	OVX	1340:1342	arg1	model					1361:1365	a critical-sized OVX calvarial defect model	1323:1365	a critical-sized OVX calvarial defect model	1323:1365	Furthermore, a critical-sized OVX calvarial defect model was used to assess the bone regeneration capability of mSIS-heparin-P28 in vivo.					
29440901	5	83	theme	heparinized	954:964	arg1	mSIS					966:969	heparinized mSIS	954:969	heparinized mSIS	954:969	These mSIS/P28 membranes were obtained through the mineralization of SIS (mSIS), followed by P28 loading onto heparinized mSIS.					
29440901	7	84	from	viability	1168:1176	arg1	rBMSCs-OVX					1270:1279	rBMSCs-OVX	1270:1279	rBMSCs-OVX	1270:1279	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	84	from	viability	1168:1176	arg1	rats					1264:1267	ovariectomized rats	1249:1267	ovariectomized rats (rBMSCs-OVX)	1249:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	8	85	theme	defect	1354:1359	arg1	model					1361:1365	a critical-sized OVX calvarial defect model	1323:1365	a critical-sized OVX calvarial defect model	1323:1365	Furthermore, a critical-sized OVX calvarial defect model was used to assess the bone regeneration capability of mSIS-heparin-P28 in vivo.					
29440901	0	86	theme	osteoporotic	7:18	arg1	regeneration					25:36	Guided osteoporotic bone regeneration	0:36	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane	0:97	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide.					
29440901	9	87	theme	release	1569:1575	arg1	time					1577:1580	a long-term release time	1557:1580	a long-term release time of 40 days	1557:1591	RESULTS In vitro results showed that P28 release from mSIS-heparin-P28 occurred in a controlled manner, with a long-term release time of 40 days.					
29440901	2	88	theme	bioactive	446:454	arg1	membranes					456:464	bioactive membranes	446:464	bioactive membranes	446:464	The guided bone regeneration (GBR) technology provides a promising strategy to cure osteoporotic defects using bioactive membranes.					
29440901	4	89	theme	BMP2	740:743	arg1	mSIS/P28					767:774	mSIS/P28	767:774	mSIS/P28	767:774	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	89	theme	BMP2	740:743	arg1	P28					762:764	bone morphogenetic protein 2 (BMP2)-related peptide P28	710:764	bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	710:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	1	90	theme	bone	296:299	arg1	capacity					267:274	the lower regeneration capacity	244:274	the lower regeneration capacity of the osteoporotic bone as compared with the normal bone	244:332	INTRODUCTION At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone.					
29440901	12	91	theme	heparinized	1934:1944	arg1	membrane					1996:2003	a potential GBR membrane	1980:2003	a potential GBR membrane for repairing osteoporotic defects	1980:2038	CONCLUSION The heparinized mSIS loaded with P28 may serve as a potential GBR membrane for repairing osteoporotic defects.					
29440901	12	91	theme	heparinized	1934:1944	arg1	mSIS					1946:1949	The heparinized mSIS	1930:1949	The heparinized mSIS loaded with P28	1930:1965	CONCLUSION The heparinized mSIS loaded with P28 may serve as a potential GBR membrane for repairing osteoporotic defects.					
29440901	4	92	theme	-related	745:752	arg1	mSIS/P28					767:774	mSIS/P28	767:774	mSIS/P28	767:774	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	92	theme	-related	745:752	arg1	P28					762:764	bone morphogenetic protein 2 (BMP2)-related peptide P28	710:764	bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	710:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	0	93	theme	composite	43:51	arg1	scaffolds					53:61	composite scaffolds	43:61	composite scaffolds of mineralized ECM/heparin membrane	43:97	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide.					
29440901	9	94	theme	days	1588:1591	arg1	time					1577:1580	a long-term release time	1557:1580	a long-term release time of 40 days	1557:1591	RESULTS In vitro results showed that P28 release from mSIS-heparin-P28 occurred in a controlled manner, with a long-term release time of 40 days.					
29440901	10	95	theme	alkaline	1664:1671	arg1	phosphatase					1673:1683	alkaline phosphatase	1664:1683	alkaline phosphatase activity	1664:1692	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	5	96	dep	obtained	874:881	arg1	followed					925:932	followed	925:932	followed by P28 loading onto heparinized mSIS	925:969	These mSIS/P28 membranes were obtained through the mineralization of SIS (mSIS), followed by P28 loading onto heparinized mSIS.					
29440901	0	97	with	regeneration	25:36	arg1	scaffolds					53:61	composite scaffolds	43:61	composite scaffolds of mineralized ECM/heparin membrane	43:97	Guided osteoporotic bone regeneration with composite scaffolds of mineralized ECM/heparin membrane loaded with BMP2-related peptide.					
29440901	8	98	used	used	1371:1374	arg2	model					1361:1365	a critical-sized OVX calvarial defect model	1323:1365	a critical-sized OVX calvarial defect model	1323:1365	Furthermore, a critical-sized OVX calvarial defect model was used to assess the bone regeneration capability of mSIS-heparin-P28 in vivo.					
29440901	7	99	from	mSIS-heparin-P28	1113:1128	arg1	differentiation					1194:1208	osteogenic differentiation	1183:1208	osteogenic differentiation	1183:1208	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	99	from	mSIS-heparin-P28	1113:1128	arg1	viability					1168:1176	viability	1168:1176	viability	1168:1176	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	99	from	mSIS-heparin-P28	1113:1128	arg1	proliferation					1153:1165	proliferation	1153:1165	proliferation	1153:1165	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	99	from	mSIS-heparin-P28	1113:1128	arg1	release					1100:1106	P28 release	1096:1106	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX)	1096:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	8	100	theme	regeneration	1395:1406	arg1	capability					1408:1417	the bone regeneration capability	1386:1417	the bone regeneration capability of mSIS-heparin-P28	1386:1437	Furthermore, a critical-sized OVX calvarial defect model was used to assess the bone regeneration capability of mSIS-heparin-P28 in vivo.					
29440901	3	101	theme	intestinal	508:517	arg1	SIS					530:532	SIS	530:532	SIS	530:532	The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response.					
29440901	3	101	theme	intestinal	508:517	arg1	submucosa					519:527	the small intestinal submucosa	498:527	the small intestinal submucosa (SIS)	498:533	The decellularized matrix from the small intestinal submucosa (SIS) has gained popularity for its natural microenvironment, which induces cell response.					
29440901	10	102	theme	mRNA	1699:1702	arg1	expression					1704:1713	mRNA expression	1699:1713	mRNA expression of osteogenesis-related genes in rBMSCs-OVX	1699:1757	Moreover, mSIS-heparin-P28 promoted cell proliferation and viability, alkaline phosphatase activity, and mRNA expression of osteogenesis-related genes in rBMSCs-OVX without the addition of extra osteogenic components.					
29440901	7	103	theme	ovariectomized	1249:1262	arg1	rBMSCs-OVX					1270:1279	rBMSCs-OVX	1270:1279	rBMSCs-OVX	1270:1279	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	7	103	theme	ovariectomized	1249:1262	arg1	rats					1264:1267	ovariectomized rats	1249:1267	ovariectomized rats (rBMSCs-OVX)	1249:1280	P28 release from mSIS-heparin-P28 and its effects on the proliferation, viability, and osteogenic differentiation of bone marrow stromal stem cells from ovariectomized rats (rBMSCs-OVX) were investigated in vitro.					
29440901	4	104	theme	novel	782:786	arg1	SIS					694:696	heparinized mineralized SIS	670:696	heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28)	670:775	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	4	104	theme	novel	782:786	arg1	membrane					792:799	a novel GBR membrane	780:799	a novel GBR membrane for guided osteoporotic bone regeneration	780:841	MATERIALS AND METHODS In this study, we developed heparinized mineralized SIS loaded with bone morphogenetic protein 2 (BMP2)-related peptide P28 (mSIS/P28) as a novel GBR membrane for guided osteoporotic bone regeneration.					
29440901	2	105	theme	GBR	365:367	arg1	technology					370:379	The guided bone regeneration (GBR) technology	335:379	The guided bone regeneration (GBR) technology	335:379	The guided bone regeneration (GBR) technology provides a promising strategy to cure osteoporotic defects using bioactive membranes.					
29440901	1	106	theme	lower	248:252	arg1	capacity					267:274	the lower regeneration capacity	244:274	the lower regeneration capacity of the osteoporotic bone as compared with the normal bone	244:332	INTRODUCTION At present, the treatment of osteoporotic defects poses a great challenge to clinicians, owing to the lower regeneration capacity of the osteoporotic bone as compared with the normal bone.					
31361522	6	0	theme	ATPSs	1223:1227	arg1	behavior					1185:1192	the flow behavior	1176:1192	the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper	1176:1247	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	4	1	theme	polymer-polymer	848:862	arg1	ATPSs					864:868	polymer-polymer ATPSs	848:868	polymer-polymer ATPSs in which the characteristics of the two phases appear quite similar	848:936	This is especially true for polymer-polymer ATPSs in which the characteristics of the two phases appear quite similar.					
31361522	9	2	theme	separation	1676:1685	arg1	phenomenon					1687:1696	the phase separation phenomenon	1666:1696	the phase separation phenomenon in hydrogels	1666:1709	We also, for the first time, successfully demonstrated the phase separation phenomenon in hydrogels, thereby extending its application and potential benefits to an alternative porous medium.					
31361522	8	3	theme	lagging	1595:1601	arg1	phases					1603:1608	the leading and lagging phases	1579:1608	the leading and lagging phases	1579:1608	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	0	4	theme	Porous	81:86	arg1	Media					88:92	Porous Media	81:92	Porous Media	81:92	Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media.					
31361522	3	5	theme	porous	672:677	arg1	media					679:683	porous media	672:683	porous media	672:683	However, since the mechanism of phase separation in porous media is not completely understood, introducing other ATPSs to paper is an unpredictable process that relies on trial and error experiments.					
31361522	3	6	dep	trial	791:795	arg1	experiments					807:817	experiments	807:817	experiments	807:817	However, since the mechanism of phase separation in porous media is not completely understood, introducing other ATPSs to paper is an unpredictable process that relies on trial and error experiments.					
31361522	5	7	from	phase	1033:1037	arg1	paper					1051:1055	paper	1051:1055	paper	1051:1055	Therefore, our group aimed to develop semiquantitative guidelines for choosing ATPSs that can phase separate in paper.					
31361522	9	8	theme	phase	1670:1674	arg1	phenomenon					1687:1696	the phase separation phenomenon	1666:1696	the phase separation phenomenon in hydrogels	1666:1709	We also, for the first time, successfully demonstrated the phase separation phenomenon in hydrogels, thereby extending its application and potential benefits to an alternative porous medium.					
31361522	8	9	theme	-dextran	1524:1531	arg1	ATPSs					1533:1537	polyethylene glycol (PEG)-dextran ATPSs	1499:1537	polyethylene glycol (PEG)-dextran ATPSs	1499:1537	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	8	10	theme	ATPSs	1533:1537	arg1	capabilities					1483:1494	the phase separation capabilities	1462:1494	the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper	1462:1546	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	9	11	theme	first	1628:1632	arg1	time					1634:1637	the first time	1624:1637	the first time	1624:1637	We also, for the first time, successfully demonstrated the phase separation phenomenon in hydrogels, thereby extending its application and potential benefits to an alternative porous medium.					
31361522	8	12	theme	glycol	1512:1517	arg1	ATPSs					1533:1537	polyethylene glycol (PEG)-dextran ATPSs	1499:1537	polyethylene glycol (PEG)-dextran ATPSs	1499:1537	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	8	13	theme	phases	1603:1608	arg1	composition					1564:1574	the composition	1560:1574	the composition of the leading and lagging phases	1560:1608	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	5	14	dep	phase	1033:1037	arg1	separate					1039:1046	separate	1039:1046	separate	1039:1046	Therefore, our group aimed to develop semiquantitative guidelines for choosing ATPSs that can phase separate in paper.					
31361522	2	15	theme	new	490:492	arg1	applications					494:505	new applications	490:505	new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components	490:617	While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components.					
31361522	8	16	theme	polyethylene	1499:1510	arg1	glycol					1512:1517	polyethylene glycol	1499:1517	polyethylene glycol (PEG)-dextran ATPSs	1499:1537	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	8	16	theme	polyethylene	1499:1510	arg1	PEG					1520:1522	PEG	1520:1522	PEG	1520:1522	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	6	17	theme	mathematical	1141:1152	arg1	equation					1098:1105	the Washburn equation	1085:1105	the Washburn equation	1085:1105	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	6	17	theme	mathematical	1141:1152	arg1	framework					1154:1162	a potential mathematical framework	1129:1162	a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper	1129:1247	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	6	17	theme	mathematical	1141:1152	arg1	parameters					1115:1124	its parameters	1111:1124	its parameters	1111:1124	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	7	18	theme	viscosity	1314:1322	arg1	determinant					1363:1373	a key determinant	1357:1373	a key determinant of the potential for phase separation in paper	1357:1420	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	7	18	theme	viscosity	1314:1322	arg1	difference					1324:1333	the viscosity difference	1310:1333	the viscosity difference between the phases	1310:1352	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	2	19	theme	lateral-flow	424:435	arg1	immunoassay					437:447	the lateral-flow immunoassay	420:447	the lateral-flow immunoassay	420:447	While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components.					
31361522	2	20	theme	different	450:458	arg1	ATPSs					460:464	different ATPSs	450:464	different ATPSs	450:464	While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components.					
31361522	6	21	theme	potential	1131:1139	arg1	equation					1098:1105	the Washburn equation	1085:1105	the Washburn equation	1085:1105	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	6	21	theme	potential	1131:1139	arg1	framework					1154:1162	a potential mathematical framework	1129:1162	a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper	1129:1247	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	6	21	theme	potential	1131:1139	arg1	parameters					1115:1124	its parameters	1111:1124	its parameters	1111:1124	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	1	22	theme	mixed	158:162	arg1	ATPS					221:224	ATPS	221:224	ATPS	221:224	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	1	22	theme	mixed	158:162	arg1	system					213:218	a mixed polymer-salt or mixed micellar aqueous two-phase system	156:218	a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS)	156:225	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	0	23	theme	Macroscopic	12:22	arg1	Separation					30:39	Macroscopic Phase Separation	12:39	Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media	12:92	Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media.					
31361522	3	24	dep	process	768:774	arg1	understood					703:712	understood	703:712	is not completely understood	685:712	However, since the mechanism of phase separation in porous media is not completely understood, introducing other ATPSs to paper is an unpredictable process that relies on trial and error experiments.					
31361522	7	25	theme	phase	1267:1271	arg1	characteristics					1279:1293	bulk phase fluid characteristics	1262:1293	bulk phase fluid characteristics	1262:1293	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	8	26	used	used	1431:1434	arg2	We					1423:1424	We	1423:1424	We	1423:1424	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	1	27	theme	polymer-salt	164:175	arg1	ATPS					221:224	ATPS	221:224	ATPS	221:224	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	1	27	theme	polymer-salt	164:175	arg1	system					213:218	a mixed polymer-salt or mixed micellar aqueous two-phase system	156:218	a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS)	156:225	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	3	28	from	mechanism	639:647	arg1	media					679:683	porous media	672:683	porous media	672:683	However, since the mechanism of phase separation in porous media is not completely understood, introducing other ATPSs to paper is an unpredictable process that relies on trial and error experiments.					
31361522	1	29	theme	constituent	250:260	arg1	phases					262:267	two constituent phases	246:267	its two constituent phases	242:267	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	2	30	theme	sample	601:606	arg1	components					608:617	sample components	601:617	sample components	601:617	While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components.					
31361522	7	31	theme	phase	1396:1400	arg1	separation					1402:1411	phase separation	1396:1411	phase separation in paper	1396:1420	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	9	32	theme	alternative	1775:1785	arg1	medium					1794:1799	an alternative porous medium	1772:1799	an alternative porous medium	1772:1799	We also, for the first time, successfully demonstrated the phase separation phenomenon in hydrogels, thereby extending its application and potential benefits to an alternative porous medium.					
31361522	7	33	theme	key	1359:1361	arg1	determinant					1363:1373	a key determinant	1357:1373	a key determinant of the potential for phase separation in paper	1357:1420	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	7	33	theme	key	1359:1361	arg1	difference					1324:1333	the viscosity difference	1310:1333	the viscosity difference between the phases	1310:1352	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	1	34	theme	previous	98:105	arg1	work					107:110	previous work	98:110	previous work	98:110	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	1	35	theme	mixed	180:184	arg1	ATPS					221:224	ATPS	221:224	ATPS	221:224	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	1	35	theme	mixed	180:184	arg1	system					213:218	a mixed polymer-salt or mixed micellar aqueous two-phase system	156:218	a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS)	156:225	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	0	36	theme	Phase	24:28	arg1	Separation					30:39	Macroscopic Phase Separation	12:39	Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media	12:92	Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media.					
31361522	3	37	theme	other	727:731	arg1	ATPSs					733:737	other ATPSs	727:737	other ATPSs	727:737	However, since the mechanism of phase separation in porous media is not completely understood, introducing other ATPSs to paper is an unpredictable process that relies on trial and error experiments.					
31361522	7	38	theme	potential	1382:1390	arg1	determinant					1363:1373	a key determinant	1357:1373	a key determinant of the potential for phase separation in paper	1357:1420	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	7	38	theme	potential	1382:1390	arg1	difference					1324:1333	the viscosity difference	1310:1333	the viscosity difference between the phases	1310:1352	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	1	39	theme	micellar	186:193	arg1	ATPS					221:224	ATPS	221:224	ATPS	221:224	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	1	39	theme	micellar	186:193	arg1	system					213:218	a mixed polymer-salt or mixed micellar aqueous two-phase system	156:218	a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS)	156:225	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	0	40	theme	Aqueous	44:50	arg1	Systems					70:76	Aqueous Two-Phase Polymer Systems	44:76	Aqueous Two-Phase Polymer Systems	44:76	Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media.					
31361522	3	41	theme	phase	652:656	arg1	separation					658:667	phase separation	652:667	phase separation	652:667	However, since the mechanism of phase separation in porous media is not completely understood, introducing other ATPSs to paper is an unpredictable process that relies on trial and error experiments.					
31361522	6	42	from	behavior	1185:1192	arg1	paper					1243:1247	fiberglass paper	1232:1247	fiberglass paper	1232:1247	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	6	43	theme	micellar	1214:1221	arg1	ATPSs					1223:1227	polymer-salt and micellar ATPSs	1197:1227	polymer-salt and micellar ATPSs	1197:1227	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	7	44	theme	fluid	1273:1277	arg1	characteristics					1279:1293	bulk phase fluid characteristics	1262:1293	bulk phase fluid characteristics	1262:1293	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	1	45	theme	aqueous	195:201	arg1	ATPS					221:224	ATPS	221:224	ATPS	221:224	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	1	45	theme	aqueous	195:201	arg1	system					213:218	a mixed polymer-salt or mixed micellar aqueous two-phase system	156:218	a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS)	156:225	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	3	46	theme	unpredictable	754:766	arg1	process					768:774	an unpredictable process	751:774	an unpredictable process that relies on trial and error experiments	751:817	However, since the mechanism of phase separation in porous media is not completely understood, introducing other ATPSs to paper is an unpredictable process that relies on trial and error experiments.					
31361522	4	47	theme	phases	910:915	arg1	characteristics					883:897	the characteristics	879:897	the characteristics of the two phases	879:915	This is especially true for polymer-polymer ATPSs in which the characteristics of the two phases appear quite similar.					
31361522	9	48	theme	potential	1750:1758	arg1	benefits					1760:1767	potential benefits	1750:1767	potential benefits	1750:1767	We also, for the first time, successfully demonstrated the phase separation phenomenon in hydrogels, thereby extending its application and potential benefits to an alternative porous medium.					
31361522	2	49	theme	target	372:377	arg1	biomarker					379:387	the target biomarker	368:387	the target biomarker	368:387	While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components.					
31361522	2	50	theme	immunoassay	437:447	arg1	sensitivity					405:415	the sensitivity	401:415	the sensitivity of the lateral-flow immunoassay	401:447	While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components.					
31361522	2	51	theme	biochemical	558:568	arg1	compatibility					570:582	biochemical compatibility	558:582	biochemical compatibility	558:582	While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components.					
31361522	0	52	theme	Systems	70:76	arg1	Separation					30:39	Macroscopic Phase Separation	12:39	Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media	12:92	Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media.					
31361522	6	53	theme	polymer-salt	1197:1208	arg1	ATPSs					1223:1227	polymer-salt and micellar ATPSs	1197:1227	polymer-salt and micellar ATPSs	1197:1227	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	8	54	from	capabilities	1483:1494	arg1	paper					1542:1546	paper	1542:1546	paper	1542:1546	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	0	55	theme	Two-Phase	52:60	arg1	Systems					70:76	Aqueous Two-Phase Polymer Systems	44:76	Aqueous Two-Phase Polymer Systems	44:76	Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media.					
31361522	8	56	theme	separation	1472:1481	arg1	capabilities					1483:1494	the phase separation capabilities	1462:1494	the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper	1462:1546	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	3	57	theme	separation	658:667	arg1	mechanism					639:647	the mechanism	635:647	the mechanism of phase separation in porous media	635:683	However, since the mechanism of phase separation in porous media is not completely understood, introducing other ATPSs to paper is an unpredictable process that relies on trial and error experiments.					
31361522	6	58	theme	fiberglass	1232:1241	arg1	paper					1243:1247	fiberglass paper	1232:1247	fiberglass paper	1232:1247	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	7	59	theme	bulk	1262:1265	arg1	characteristics					1279:1293	bulk phase fluid characteristics	1262:1293	bulk phase fluid characteristics	1262:1293	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	6	60	theme	Washburn	1089:1096	arg1	framework					1154:1162	a potential mathematical framework	1129:1162	a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper	1129:1247	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	6	60	theme	Washburn	1089:1096	arg1	equation					1098:1105	the Washburn equation	1085:1105	the Washburn equation	1085:1105	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	6	60	theme	Washburn	1089:1096	arg1	parameters					1115:1124	its parameters	1111:1124	its parameters	1111:1124	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	2	61	theme	biomarker	532:540	arg1	partitioning					542:553	biomarker partitioning	532:553	biomarker partitioning	532:553	While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components.					
31361522	6	62	theme	flow	1180:1183	arg1	behavior					1185:1192	the flow behavior	1176:1192	the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper	1176:1247	In this work, we evaluated the Washburn equation and its parameters as a potential mathematical framework to describe the flow behavior of polymer-salt and micellar ATPSs in fiberglass paper.					
31361522	8	63	theme	phase	1466:1470	arg1	capabilities					1483:1494	the phase separation capabilities	1462:1494	the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper	1462:1546	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	1	64	theme	two-phase	203:211	arg1	ATPS					221:224	ATPS	221:224	ATPS	221:224	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	1	64	theme	two-phase	203:211	arg1	system					213:218	a mixed polymer-salt or mixed micellar aqueous two-phase system	156:218	a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS)	156:225	In previous work, our group discovered a phenomenon in which a mixed polymer-salt or mixed micellar aqueous two-phase system (ATPS) separates into its two constituent phases as it flows within paper.					
31361522	5	65	theme	semiquantitative	977:992	arg1	guidelines					994:1003	semiquantitative guidelines	977:1003	semiquantitative guidelines for choosing ATPSs that can phase separate in paper	977:1055	Therefore, our group aimed to develop semiquantitative guidelines for choosing ATPSs that can phase separate in paper.					
31361522	2	66	theme	respective	334:343	arg1	studies					345:351	their respective studies	328:351	their respective studies	328:351	While these ATPSs worked well in their respective studies to concentrate the target biomarker and improve the sensitivity of the lateral-flow immunoassay, different ATPSs can be advantageous for new applications based on factors such as biomarker partitioning or biochemical compatibility between ATPS and sample components.					
31361522	8	67	theme	leading	1583:1589	arg1	phases					1603:1608	the leading and lagging phases	1579:1608	the leading and lagging phases	1579:1608	We then used this parameter to predict the phase separation capabilities of polyethylene glycol (PEG)-dextran ATPSs in paper and control the composition of the leading and lagging phases.					
31361522	0	68	theme	Polymer	62:68	arg1	Systems					70:76	Aqueous Two-Phase Polymer Systems	44:76	Aqueous Two-Phase Polymer Systems	44:76	Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media.					
31361522	0	69	from	Separation	30:39	arg1	Media					88:92	Porous Media	81:92	Porous Media	81:92	Controlling Macroscopic Phase Separation of Aqueous Two-Phase Polymer Systems in Porous Media.					
31361522	9	70	theme	porous	1787:1792	arg1	medium					1794:1799	an alternative porous medium	1772:1799	an alternative porous medium	1772:1799	We also, for the first time, successfully demonstrated the phase separation phenomenon in hydrogels, thereby extending its application and potential benefits to an alternative porous medium.					
31361522	7	71	from	separation	1402:1411	arg1	paper					1416:1420	paper	1416:1420	paper	1416:1420	We compared bulk phase fluid characteristics and identified the viscosity difference between the phases as a key determinant of the potential for phase separation in paper.					
31361522	9	72	from	phenomenon	1687:1696	arg1	hydrogels					1701:1709	hydrogels	1701:1709	hydrogels	1701:1709	We also, for the first time, successfully demonstrated the phase separation phenomenon in hydrogels, thereby extending its application and potential benefits to an alternative porous medium.					
31434476	6	0	theme	minimal	855:861	arg1	toxicity					863:870	minimal toxicity	855:870	minimal toxicity	855:870	They also exhibited minimal toxicity in vitro and showed transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa).					
31434476	3	1	theme	potential	592:600	arg1	measurements					602:613	zeta potential measurements	587:613	zeta potential measurements	587:613	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	1	2	theme	special	241:247	arg1	importance					249:258	special importance	241:258	special importance	241:258	Among the many nonviral gene delivery vectors, chitosan, being a polysaccharide of natural origin, has gained special importance.					
31434476	11	3	theme	composite	1897:1905	arg1	formulation					1907:1917	the designed composite formulation	1884:1917	the designed composite formulation	1884:1917	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	10	4	theme	chitosan-pyridine	1638:1654	arg1	CH2COOH					1676:1682	CH2COOH	1676:1682	CH2COOH	1676:1682	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	10	4	theme	chitosan-pyridine	1638:1654	arg1	CS					1673:1674	the chitosan-pyridine imine vector (py)CS	1634:1674	the chitosan-pyridine imine vector (py)CS(CH2COOH)	1634:1683	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	5	5	theme	physiological	793:805	arg1	polyanions					807:816	physiological polyanions	793:816	physiological polyanions such as heparin	793:832	These polyplexes were stable to DNase and physiological polyanions such as heparin.					
31434476	5	5	theme	physiological	793:805	arg1	heparin					826:832	heparin	826:832	heparin	826:832	These polyplexes were stable to DNase and physiological polyanions such as heparin.					
31434476	11	6	dep	in	1838:1839	arg1	vivo					1841:1844	vivo	1841:1844	vivo	1841:1844	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	6	7	theme	comparable	912:921	arg1	levels					905:910	transfection levels	892:910	transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa)	892:1018	They also exhibited minimal toxicity in vitro and showed transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa).					
31434476	0	8	theme	Drug	125:128	arg1	Co-Delivery					101:111	Co-Delivery	101:111	Co-Delivery of Gene and Drug	101:128	New Water-Soluble Oxyamino Chitosans as Biocompatible Vectors for Efficacious Anticancer Therapy via Co-Delivery of Gene and Drug.					
31434476	10	9	theme	py	1670:1671	arg1	CH2COOH					1676:1682	CH2COOH	1676:1682	CH2COOH	1676:1682	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	10	9	theme	py	1670:1671	arg1	CS					1673:1674	the chitosan-pyridine imine vector (py)CS	1634:1674	the chitosan-pyridine imine vector (py)CS(CH2COOH)	1634:1683	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	11	10	dep	effectiveness	1846:1858	arg1	the					1834:1836	the	1834:1836	the	1834:1836	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	6	11	theme	transfection	892:903	arg1	levels					905:910	transfection levels	892:910	transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa)	892:1018	They also exhibited minimal toxicity in vitro and showed transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa).					
31434476	10	12	theme	p53	1563:1565	arg1	Co-delivery					1531:1541	Co-delivery	1531:1541	Co-delivery of functional gene (p53) and drug (doxorubicin)	1531:1589	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	12	13	theme	drug	1992:1995	arg1	release					1997:2003	slow and steady drug release	1976:2003	slow and steady drug release	1976:2003	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	10	14	theme	gene	1557:1560	arg1	p53					1563:1565	functional gene (p53)	1546:1566	functional gene (p53)	1546:1566	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	11	15	theme	in	1838:1839	arg1	effectiveness					1846:1858	in vivo effectiveness	1838:1858	in vivo effectiveness	1838:1858	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	12	16	theme	oxime	1955:1959	arg1	linkage					1961:1967	the oxime linkage	1951:1967	the oxime linkage aiding slow and steady drug release	1951:2003	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	9	17	used	used	1463:1466	arg2	sensitivity					1361:1371	The pH sensitivity	1354:1371	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions	1354:1451	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	3	18	theme	substitution	545:556	arg1	degree					526:531	the degree	522:531	the degree of pyridine substitution (47%)	522:562	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	8	19	theme	alcohols	1219:1226	arg1	alcohols					1219:1226	the primary alcohols	1207:1226	the primary alcohols	1207:1226	Nearly 40% of the primary alcohols were successfully converted to oxyamino functionality, which was used for forming oxime with the anticancer drug doxorubicin.					
31434476	8	19	theme	alcohols	1219:1226	arg1	%					1202:1202	Nearly 40%	1193:1202	Nearly 40% of the primary alcohols	1193:1226	Nearly 40% of the primary alcohols were successfully converted to oxyamino functionality, which was used for forming oxime with the anticancer drug doxorubicin.					
31434476	12	20	with	nanobiocomposite	2119:2134	arg1	potential					2183:2191	significantly high anticancer therapeutic potential	2141:2191	significantly high anticancer therapeutic potential	2141:2191	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	11	21	theme	designed	1888:1895	arg1	formulation					1907:1917	the designed composite formulation	1884:1917	the designed composite formulation	1884:1917	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	9	22	theme	relevant	1433:1440	arg1	conditions					1442:1451	biologically relevant conditions	1420:1451	biologically relevant conditions	1420:1451	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	12	23	theme	pH	1933:1934	arg1	sensitivity					1936:1946	the pH sensitivity	1929:1946	the pH sensitivity of the oxime linkage aiding slow and steady drug release	1929:2003	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	0	24	theme	Efficacious	66:76	arg1	Therapy					89:95	Efficacious Anticancer Therapy	66:95	Efficacious Anticancer Therapy	66:95	New Water-Soluble Oxyamino Chitosans as Biocompatible Vectors for Efficacious Anticancer Therapy via Co-Delivery of Gene and Drug.					
31434476	3	25	theme	pyridine	536:543	arg1	substitution					545:556	pyridine substitution	536:556	pyridine substitution (47%)	536:562	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	3	25	theme	pyridine	536:543	arg1	%					561:561	47%	559:561	47%	559:561	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	1	26	theme	nonviral	146:153	arg1	vectors					169:175	the many nonviral gene delivery vectors	137:175	the many nonviral gene delivery vectors	137:175	Among the many nonviral gene delivery vectors, chitosan, being a polysaccharide of natural origin, has gained special importance.					
31434476	1	27	theme	delivery	160:167	arg1	vectors					169:175	the many nonviral gene delivery vectors	137:175	the many nonviral gene delivery vectors	137:175	Among the many nonviral gene delivery vectors, chitosan, being a polysaccharide of natural origin, has gained special importance.					
31434476	6	28	theme	standard	941:948	arg1	2000					964:967	the commercial standard Lipofectamine 2000	926:967	the commercial standard Lipofectamine 2000	926:967	They also exhibited minimal toxicity in vitro and showed transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa).					
31434476	10	29	theme	doxorubicin	1711:1721	arg1	Dox					1738:1740	Dox	1738:1740	Dox	1738:1740	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	10	29	theme	doxorubicin	1711:1721	arg1	CS					1735:1736	the newly synthesized doxorubicin oxime ether CS	1689:1736	the newly synthesized doxorubicin oxime ether CS(Dox)	1689:1741	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	6	30	dep	polyethylenimine	990:1005	arg1	MW					1008:1009	MW	1008:1009	MW	1008:1009	They also exhibited minimal toxicity in vitro and showed transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa).					
31434476	6	30	dep	polyethylenimine	990:1005	arg1	kDa					1015:1017	25 kDa	1012:1017	25 kDa	1012:1017	They also exhibited minimal toxicity in vitro and showed transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa).					
31434476	10	31	theme	vector	1662:1667	arg1	CH2COOH					1676:1682	CH2COOH	1676:1682	CH2COOH	1676:1682	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	10	31	theme	vector	1662:1667	arg1	CS					1673:1674	the chitosan-pyridine imine vector (py)CS	1634:1674	the chitosan-pyridine imine vector (py)CS(CH2COOH)	1634:1683	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	12	32	theme	linkage	1961:1967	arg1	sensitivity					1936:1946	the pH sensitivity	1929:1946	the pH sensitivity of the oxime linkage aiding slow and steady drug release	1929:2003	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	10	33	theme	drug	1572:1575	arg1	doxorubicin					1578:1588	drug (doxorubicin)	1572:1589	drug (doxorubicin)	1572:1589	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	0	34	theme	Gene	116:119	arg1	Co-Delivery					101:111	Co-Delivery	101:111	Co-Delivery of Gene and Drug	101:128	New Water-Soluble Oxyamino Chitosans as Biocompatible Vectors for Efficacious Anticancer Therapy via Co-Delivery of Gene and Drug.					
31434476	0	35	theme	Biocompatible	40:52	arg1	Vectors					54:60	Biocompatible Vectors	40:60	Biocompatible Vectors for Efficacious Anticancer Therapy	40:95	New Water-Soluble Oxyamino Chitosans as Biocompatible Vectors for Efficacious Anticancer Therapy via Co-Delivery of Gene and Drug.					
31434476	12	36	theme	anticancer	2160:2169	arg1	potential					2183:2191	significantly high anticancer therapeutic potential	2141:2191	significantly high anticancer therapeutic potential	2141:2191	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	4	37	theme	gene	656:659	arg1	ability					669:675	a high gene binding ability	649:675	a high gene binding ability	649:675	The optimized formulation showed a high gene binding ability, forming nanosized positively charged polyelectrolyte complexes with DNA.					
31434476	11	38	theme	appreciable	1799:1809	arg1	survivability					1811:1823	appreciable survivability	1799:1823	appreciable survivability	1799:1823	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	7	39	theme	chitosan	1092:1099	arg1	derivative					1078:1087	a hitherto unknown oxyamine derivative	1050:1087	a hitherto unknown oxyamine derivative of chitosan	1050:1099	Additionally, in this study, a hitherto unknown oxyamine derivative of chitosan has been prepared by phthaloyl protection, tosylation, and Gabriel's phthalimide synthesis.					
31434476	10	40	theme	imine	1656:1660	arg1	CH2COOH					1676:1682	CH2COOH	1676:1682	CH2COOH	1676:1682	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	10	40	theme	imine	1656:1660	arg1	CS					1673:1674	the chitosan-pyridine imine vector (py)CS	1634:1674	the chitosan-pyridine imine vector (py)CS(CH2COOH)	1634:1683	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	9	41	theme	drug	1509:1512	arg1	compound					1485:1492	the compound	1481:1492	the compound	1481:1492	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	9	41	theme	drug	1509:1512	arg1	vector					1523:1528	a versatile drug delivery vector	1497:1528	a versatile drug delivery vector	1497:1528	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	12	42	theme	pyridine-tethered	2053:2069	arg1	chitosan					2085:2092	pyridine-tethered carboxymethyl chitosan	2053:2092	pyridine-tethered carboxymethyl chitosan	2053:2092	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	10	43	theme	doxorubicin	1578:1588	arg1	Co-delivery					1531:1541	Co-delivery	1531:1541	Co-delivery of functional gene (p53) and drug (doxorubicin)	1531:1589	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	2	44	theme	carboxymethylation	405:422	arg1	degree					395:400	an optimum degree	384:400	an optimum degree of carboxymethylation	384:422	In this report, chitosan (CS) has been solubilized in water by preparing its O-carboxymethyl derivative, CS(CH2COOH), with an optimum degree of carboxymethylation.					
31434476	10	45	theme	ether	1729:1733	arg1	Dox					1738:1740	Dox	1738:1740	Dox	1738:1740	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	10	45	theme	ether	1729:1733	arg1	CS					1735:1736	the newly synthesized doxorubicin oxime ether CS	1689:1736	the newly synthesized doxorubicin oxime ether CS(Dox)	1689:1741	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	8	46	theme	anticancer	1325:1334	arg1	doxorubicin					1341:1351	the anticancer drug doxorubicin	1321:1351	the anticancer drug doxorubicin	1321:1351	Nearly 40% of the primary alcohols were successfully converted to oxyamino functionality, which was used for forming oxime with the anticancer drug doxorubicin.					
31434476	7	47	theme	unknown	1061:1067	arg1	derivative					1078:1087	a hitherto unknown oxyamine derivative	1050:1087	a hitherto unknown oxyamine derivative of chitosan	1050:1099	Additionally, in this study, a hitherto unknown oxyamine derivative of chitosan has been prepared by phthaloyl protection, tosylation, and Gabriel's phthalimide synthesis.					
31434476	3	48	theme	zeta	587:590	arg1	measurements					602:613	zeta potential measurements	587:613	zeta potential measurements	587:613	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	4	49	theme	polyelectrolyte	715:729	arg1	complexes					731:739	nanosized positively charged polyelectrolyte complexes	686:739	nanosized positively charged polyelectrolyte complexes	686:739	The optimized formulation showed a high gene binding ability, forming nanosized positively charged polyelectrolyte complexes with DNA.					
31434476	1	50	theme	natural	214:220	arg1	origin					222:227	natural origin	214:227	natural origin	214:227	Among the many nonviral gene delivery vectors, chitosan, being a polysaccharide of natural origin, has gained special importance.					
31434476	11	51	theme	Complete	1744:1751	arg1	regression					1759:1768	Complete tumor regression	1744:1768	Complete tumor regression with no tumor recurrence and appreciable survivability	1744:1823	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	2	52	with	derivative	354:363	arg1	degree					395:400	an optimum degree	384:400	an optimum degree of carboxymethylation	384:422	In this report, chitosan (CS) has been solubilized in water by preparing its O-carboxymethyl derivative, CS(CH2COOH), with an optimum degree of carboxymethylation.					
31434476	3	53	theme	py	500:501	arg1	CH2COOH					506:512	CH2COOH	506:512	CH2COOH	506:512	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	3	53	theme	py	500:501	arg1	CS					503:504	the pyridine-substituted product (py)CS	466:504	the pyridine-substituted product (py)CS(CH2COOH)	466:513	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	3	54	theme	product	491:497	arg1	CH2COOH					506:512	CH2COOH	506:512	CH2COOH	506:512	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	3	54	theme	product	491:497	arg1	CS					503:504	the pyridine-substituted product (py)CS	466:504	the pyridine-substituted product (py)CS(CH2COOH)	466:513	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	9	55	theme	pH	1358:1359	arg1	sensitivity					1361:1371	The pH sensitivity	1354:1371	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions	1354:1451	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	11	56	theme	formulation	1907:1917	arg1	effectiveness					1846:1858	in vivo effectiveness	1838:1858	in vivo effectiveness	1838:1858	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	11	56	theme	formulation	1907:1917	arg1	biocompatibility					1864:1879	biocompatibility	1864:1879	biocompatibility	1864:1879	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	9	57	theme	oxime	1380:1384	arg1	linkage					1392:1398	the oxime ether linkage	1376:1398	the oxime ether linkage	1376:1398	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	12	58	theme	slow	1976:1979	arg1	release					1997:2003	slow and steady drug release	1976:2003	slow and steady drug release	1976:2003	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	9	59	theme	linkage	1392:1398	arg1	sensitivity					1361:1371	The pH sensitivity	1354:1371	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions	1354:1451	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	12	60	theme	steady	1985:1990	arg1	release					1997:2003	slow and steady drug release	1976:2003	slow and steady drug release	1976:2003	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	10	61	theme	functional	1546:1555	arg1	p53					1563:1565	functional gene (p53)	1546:1566	functional gene (p53)	1546:1566	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	11	62	with	regression	1759:1768	arg1	recurrence					1784:1793	no tumor recurrence	1775:1793	no tumor recurrence	1775:1793	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	11	62	with	regression	1759:1768	arg1	survivability					1811:1823	appreciable survivability	1799:1823	appreciable survivability	1799:1823	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	9	63	theme	stability	1404:1412	arg1	sensitivity					1361:1371	The pH sensitivity	1354:1371	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions	1354:1451	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	4	64	theme	optimized	620:628	arg1	formulation					630:640	The optimized formulation	616:640	The optimized formulation	616:640	The optimized formulation showed a high gene binding ability, forming nanosized positively charged polyelectrolyte complexes with DNA.					
31434476	12	65	theme	sustained	2024:2032	arg1	expression					2039:2048	the sustained gene expression	2020:2048	the sustained gene expression by pyridine-tethered carboxymethyl chitosan	2020:2092	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	10	66	theme	oxime	1723:1727	arg1	Dox					1738:1740	Dox	1738:1740	Dox	1738:1740	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	10	66	theme	oxime	1723:1727	arg1	CS					1735:1736	the newly synthesized doxorubicin oxime ether CS	1689:1736	the newly synthesized doxorubicin oxime ether CS(Dox)	1689:1741	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	6	67	theme	higher	978:983	arg1	levels					905:910	transfection levels	892:910	transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa)	892:1018	They also exhibited minimal toxicity in vitro and showed transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa).					
31434476	8	68	theme	primary	1211:1217	arg1	alcohols					1219:1226	the primary alcohols	1207:1226	the primary alcohols	1207:1226	Nearly 40% of the primary alcohols were successfully converted to oxyamino functionality, which was used for forming oxime with the anticancer drug doxorubicin.					
31434476	0	69	theme	Anticancer	78:87	arg1	Therapy					89:95	Efficacious Anticancer Therapy	66:95	Efficacious Anticancer Therapy	66:95	New Water-Soluble Oxyamino Chitosans as Biocompatible Vectors for Efficacious Anticancer Therapy via Co-Delivery of Gene and Drug.					
31434476	10	70	theme	synthesized	1699:1709	arg1	Dox					1738:1740	Dox	1738:1740	Dox	1738:1740	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	10	70	theme	synthesized	1699:1709	arg1	CS					1735:1736	the newly synthesized doxorubicin oxime ether CS	1689:1736	the newly synthesized doxorubicin oxime ether CS(Dox)	1689:1741	Co-delivery of functional gene (p53) and drug (doxorubicin) was accomplished in vitro and in vivo with the chitosan-pyridine imine vector (py)CS(CH2COOH) and the newly synthesized doxorubicin oxime ether CS(Dox).					
31434476	1	71	theme	many	141:144	arg1	vectors					169:175	the many nonviral gene delivery vectors	137:175	the many nonviral gene delivery vectors	137:175	Among the many nonviral gene delivery vectors, chitosan, being a polysaccharide of natural origin, has gained special importance.					
31434476	1	72	theme	gene	155:158	arg1	vectors					169:175	the many nonviral gene delivery vectors	137:175	the many nonviral gene delivery vectors	137:175	Among the many nonviral gene delivery vectors, chitosan, being a polysaccharide of natural origin, has gained special importance.					
31434476	6	73	theme	Lipofectamine	950:962	arg1	2000					964:967	the commercial standard Lipofectamine 2000	926:967	the commercial standard Lipofectamine 2000	926:967	They also exhibited minimal toxicity in vitro and showed transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa).					
31434476	7	74	theme	phthaloyl	1122:1130	arg1	protection					1132:1141	phthaloyl protection	1122:1141	phthaloyl protection	1122:1141	Additionally, in this study, a hitherto unknown oxyamine derivative of chitosan has been prepared by phthaloyl protection, tosylation, and Gabriel's phthalimide synthesis.					
31434476	4	75	theme	charged	707:713	arg1	complexes					731:739	nanosized positively charged polyelectrolyte complexes	686:739	nanosized positively charged polyelectrolyte complexes	686:739	The optimized formulation showed a high gene binding ability, forming nanosized positively charged polyelectrolyte complexes with DNA.					
31434476	6	76	theme	commercial	930:939	arg1	2000					964:967	the commercial standard Lipofectamine 2000	926:967	the commercial standard Lipofectamine 2000	926:967	They also exhibited minimal toxicity in vitro and showed transfection levels comparable to the commercial standard Lipofectamine 2000 and much higher than polyethylenimine (MW, 25 kDa).					
31434476	12	77	theme	high	2155:2158	arg1	potential					2183:2191	significantly high anticancer therapeutic potential	2141:2191	significantly high anticancer therapeutic potential	2141:2191	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	8	78	theme	oxyamino	1259:1266	arg1	functionality					1268:1280	oxyamino functionality	1259:1280	oxyamino functionality	1259:1280	Nearly 40% of the primary alcohols were successfully converted to oxyamino functionality, which was used for forming oxime with the anticancer drug doxorubicin.					
31434476	9	79	theme	ether	1386:1390	arg1	linkage					1392:1398	the oxime ether linkage	1376:1398	the oxime ether linkage	1376:1398	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	12	80	theme	therapeutic	2171:2181	arg1	potential					2183:2191	significantly high anticancer therapeutic potential	2141:2191	significantly high anticancer therapeutic potential	2141:2191	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	4	81	theme	nanosized	686:694	arg1	complexes					731:739	nanosized positively charged polyelectrolyte complexes	686:739	nanosized positively charged polyelectrolyte complexes	686:739	The optimized formulation showed a high gene binding ability, forming nanosized positively charged polyelectrolyte complexes with DNA.					
31434476	11	82	theme	tumor	1778:1782	arg1	recurrence					1784:1793	no tumor recurrence	1775:1793	no tumor recurrence	1775:1793	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
31434476	2	83	theme	optimum	387:393	arg1	degree					395:400	an optimum degree	384:400	an optimum degree of carboxymethylation	384:422	In this report, chitosan (CS) has been solubilized in water by preparing its O-carboxymethyl derivative, CS(CH2COOH), with an optimum degree of carboxymethylation.					
31434476	4	84	theme	high	651:654	arg1	ability					669:675	a high gene binding ability	649:675	a high gene binding ability	649:675	The optimized formulation showed a high gene binding ability, forming nanosized positively charged polyelectrolyte complexes with DNA.					
31434476	2	85	theme	O-carboxymethyl	338:352	arg1	CS					366:367	CS	366:367	CS(CH2COOH)	366:376	In this report, chitosan (CS) has been solubilized in water by preparing its O-carboxymethyl derivative, CS(CH2COOH), with an optimum degree of carboxymethylation.					
31434476	2	85	theme	O-carboxymethyl	338:352	arg1	derivative					354:363	its O-carboxymethyl derivative	334:363	its O-carboxymethyl derivative	334:363	In this report, chitosan (CS) has been solubilized in water by preparing its O-carboxymethyl derivative, CS(CH2COOH), with an optimum degree of carboxymethylation.					
31434476	9	86	theme	versatile	1499:1507	arg1	compound					1485:1492	the compound	1481:1492	the compound	1481:1492	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	9	86	theme	versatile	1499:1507	arg1	vector					1523:1528	a versatile drug delivery vector	1497:1528	a versatile drug delivery vector	1497:1528	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	4	87	theme	binding	661:667	arg1	ability					669:675	a high gene binding ability	649:675	a high gene binding ability	649:675	The optimized formulation showed a high gene binding ability, forming nanosized positively charged polyelectrolyte complexes with DNA.					
31434476	7	88	theme	oxyamine	1069:1076	arg1	derivative					1078:1087	a hitherto unknown oxyamine derivative	1050:1087	a hitherto unknown oxyamine derivative of chitosan	1050:1099	Additionally, in this study, a hitherto unknown oxyamine derivative of chitosan has been prepared by phthaloyl protection, tosylation, and Gabriel's phthalimide synthesis.					
31434476	9	89	theme	delivery	1514:1521	arg1	compound					1485:1492	the compound	1481:1492	the compound	1481:1492	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	9	89	theme	delivery	1514:1521	arg1	vector					1523:1528	a versatile drug delivery vector	1497:1528	a versatile drug delivery vector	1497:1528	The pH sensitivity of the oxime ether linkage and stability under biologically relevant conditions were then used to establish the compound as a versatile drug delivery vector.					
31434476	8	90	used	used	1293:1296	arg2	functionality					1268:1280	oxyamino functionality	1259:1280	oxyamino functionality	1259:1280	Nearly 40% of the primary alcohols were successfully converted to oxyamino functionality, which was used for forming oxime with the anticancer drug doxorubicin.					
31434476	12	91	theme	carboxymethyl	2071:2083	arg1	chitosan					2085:2092	pyridine-tethered carboxymethyl chitosan	2053:2092	pyridine-tethered carboxymethyl chitosan	2053:2092	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	8	92	theme	drug	1336:1339	arg1	doxorubicin					1341:1351	the anticancer drug doxorubicin	1321:1351	the anticancer drug doxorubicin	1321:1351	Nearly 40% of the primary alcohols were successfully converted to oxyamino functionality, which was used for forming oxime with the anticancer drug doxorubicin.					
31434476	3	93	theme	pyridine-substituted	470:489	arg1	CH2COOH					506:512	CH2COOH	506:512	CH2COOH	506:512	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	3	93	theme	pyridine-substituted	470:489	arg1	CS					503:504	the pyridine-substituted product (py)CS	466:504	the pyridine-substituted product (py)CS(CH2COOH)	466:513	This has been further derivatized to get the pyridine-substituted product (py)CS(CH2COOH), where the degree of pyridine substitution (47%) was optimized based on zeta potential measurements.					
31434476	1	94	theme	origin	222:227	arg1	polysaccharide					196:209	a polysaccharide	194:209	a polysaccharide of natural origin	194:227	Among the many nonviral gene delivery vectors, chitosan, being a polysaccharide of natural origin, has gained special importance.					
31434476	7	95	theme	phthalimide	1170:1180	arg1	synthesis					1182:1190	Gabriel's phthalimide synthesis	1160:1190	Gabriel's phthalimide synthesis	1160:1190	Additionally, in this study, a hitherto unknown oxyamine derivative of chitosan has been prepared by phthaloyl protection, tosylation, and Gabriel's phthalimide synthesis.					
31434476	12	96	theme	gene	2034:2037	arg1	expression					2039:2048	the sustained gene expression	2020:2048	the sustained gene expression by pyridine-tethered carboxymethyl chitosan	2020:2092	Overall, the pH sensitivity of the oxime linkage aiding slow and steady drug release, together with the sustained gene expression by pyridine-tethered carboxymethyl chitosan, allows us to generate a nanobiocomposite with significantly high anticancer therapeutic potential.					
31434476	11	97	theme	tumor	1753:1757	arg1	regression					1759:1768	Complete tumor regression	1744:1768	Complete tumor regression with no tumor recurrence and appreciable survivability	1744:1823	Complete tumor regression with no tumor recurrence and appreciable survivability point to the in vivo effectiveness and biocompatibility of the designed composite formulation.					
30970089	5	0	theme	*	638:638	arg1	system					640:645	the CIE L*a*b* system	625:645	the CIE L*a*b* system using a reflectance spectrophotometer	625:683	The baseline color was measured according to the CIE L*a*b* system using a reflectance spectrophotometer.					
30970089	8	1	theme	color	894:898	arg1	stability					900:908	The color stability	890:908	The color stability	890:908	The color stability was determined using the difference between the coordinates obtained before and after the aging process.					
30970089	2	2	theme	chitosan-modified	337:353	arg1	material					355:362	chitosan-modified material	337:362	chitosan-modified material	337:362	Four bisacrylic resins were evaluated, namely, control, chitosan-modified material, nanodiamond-modified material, and chitosan-nanodiamond-modified material.					
30970089	10	3	theme	chitosan	1172:1179	arg1	incorporation					1138:1150	the incorporation	1134:1150	the incorporation of nanodiamonds and chitosan into a bisacrylic resin	1134:1203	The results show that the incorporation of nanodiamonds and chitosan into a bisacrylic resin provided a better color stability to the materials (p = 0.007).					
30970089	1	4	theme	bisacrylic	233:242	arg1	resin					244:248	a bisacrylic resin	231:248	a bisacrylic resin subjected to artificial aging	231:278	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	0	5	theme	resin	87:91	arg1	stability					6:14	Color stability	0:14	Color stability	0:14	Color stability and surface roughness of chitosan- and nanodiamond-modified bisacrylic resin.					
30970089	0	5	theme	resin	87:91	arg1	roughness					28:36	surface roughness	20:36	surface roughness	20:36	Color stability and surface roughness of chitosan- and nanodiamond-modified bisacrylic resin.					
30970089	11	6	from	storage	1273:1279	arg1	wine					1288:1291	red wine	1284:1291	red wine	1284:1291	The storage in red wine resulted in a higher variation in the surface roughness values, especially when only the nanodiamond was incorporated to the material (p < 0.05).					
30970089	1	7	theme	chitosan	145:152	arg1	effect					135:140	the effect	131:140	the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging	131:278	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	12	8	theme	bisacrylic	1558:1567	arg1	resin					1569:1573	the bisacrylic resin	1554:1573	the bisacrylic resin	1554:1573	The incorporation of both chitosan and nanodiamonds are promising in providing an improvement in the properties of the bisacrylic resin when they are simultaneously incorporated in the product.					
30970089	1	9	theme	resin	244:248	arg1	stability					196:204	color stability	190:204	color stability	190:204	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	1	9	theme	resin	244:248	arg1	roughness					218:226	surface roughness	210:226	surface roughness	210:226	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	6	10	theme	distilled	779:787	arg1	n					796:796	n = 8	796:800	n = 8	796:800	After these tests, the specimens were individually immersed in cola soft drink, red wine, or distilled water (n = 8) for 28 days.					
30970089	6	10	theme	distilled	779:787	arg1	water					789:793	distilled water	779:793	distilled water (n = 8)	779:801	After these tests, the specimens were individually immersed in cola soft drink, red wine, or distilled water (n = 8) for 28 days.					
30970089	7	11	theme	final	859:863	arg1	color					865:869	final color	859:869	final color	859:869	After the aging, the surface roughness and final color were re-evaluated.					
30970089	5	12	theme	CIE	629:631	arg1	*					638:638	the CIE L*a*b*	625:638	the CIE L*a*b* system using a reflectance spectrophotometer	625:683	The baseline color was measured according to the CIE L*a*b* system using a reflectance spectrophotometer.					
30970089	11	13	theme	red	1284:1286	arg1	wine					1288:1291	red wine	1284:1291	red wine	1284:1291	The storage in red wine resulted in a higher variation in the surface roughness values, especially when only the nanodiamond was incorporated to the material (p < 0.05).					
30970089	4	14	theme	surface	499:505	arg1	roughness					507:515	The surface roughness	495:515	The surface roughness	495:515	The surface roughness was determined using a profilometer with a cut-off of 0.25 mm.					
30970089	5	15	theme	*	634:634	arg1	*					638:638	the CIE L*a*b*	625:638	the CIE L*a*b* system using a reflectance spectrophotometer	625:683	The baseline color was measured according to the CIE L*a*b* system using a reflectance spectrophotometer.					
30970089	1	16	from	effect	135:140	arg1	stability					196:204	color stability	190:204	color stability	190:204	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	1	16	from	effect	135:140	arg1	roughness					218:226	surface roughness	210:226	surface roughness	210:226	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	12	17	from	improvement	1521:1531	arg1	properties					1540:1549	the properties	1536:1549	the properties of the bisacrylic resin	1536:1573	The incorporation of both chitosan and nanodiamonds are promising in providing an improvement in the properties of the bisacrylic resin when they are simultaneously incorporated in the product.					
30970089	5	18	theme	a	635:635	arg1	*					638:638	the CIE L*a*b*	625:638	the CIE L*a*b* system using a reflectance spectrophotometer	625:683	The baseline color was measured according to the CIE L*a*b* system using a reflectance spectrophotometer.					
30970089	10	19	theme	bisacrylic	1188:1197	arg1	resin					1199:1203	a bisacrylic resin	1186:1203	a bisacrylic resin	1186:1203	The results show that the incorporation of nanodiamonds and chitosan into a bisacrylic resin provided a better color stability to the materials (p = 0.007).					
30970089	1	20	theme	nanodiamond	157:167	arg1	incorporation					169:181	nanodiamond incorporation	157:181	nanodiamond incorporation	157:181	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	5	21	theme	*	636:636	arg1	*					638:638	the CIE L*a*b*	625:638	the CIE L*a*b* system using a reflectance spectrophotometer	625:683	The baseline color was measured according to the CIE L*a*b* system using a reflectance spectrophotometer.					
30970089	12	22	theme	chitosan	1465:1472	arg1	incorporation					1443:1455	The incorporation	1439:1455	The incorporation of both chitosan and nanodiamonds	1439:1489	The incorporation of both chitosan and nanodiamonds are promising in providing an improvement in the properties of the bisacrylic resin when they are simultaneously incorporated in the product.					
30970089	12	22	theme	chitosan	1465:1472	arg1	promising					1495:1503	promising	1495:1503	promising	1495:1503	The incorporation of both chitosan and nanodiamonds are promising in providing an improvement in the properties of the bisacrylic resin when they are simultaneously incorporated in the product.					
30970089	11	23	theme	higher	1307:1312	arg1	variation					1314:1322	a higher variation	1305:1322	a higher variation in the surface roughness values	1305:1354	The storage in red wine resulted in a higher variation in the surface roughness values, especially when only the nanodiamond was incorporated to the material (p < 0.05).					
30970089	1	24	theme	incorporation	169:181	arg1	effect					135:140	the effect	131:140	the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging	131:278	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	0	25	theme	Color	0:4	arg1	stability					6:14	Color stability	0:14	Color stability	0:14	Color stability and surface roughness of chitosan- and nanodiamond-modified bisacrylic resin.					
30970089	1	26	theme	artificial	263:272	arg1	aging					274:278	artificial aging	263:278	artificial aging	263:278	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	5	27	theme	b	637:637	arg1	*					638:638	the CIE L*a*b*	625:638	the CIE L*a*b* system using a reflectance spectrophotometer	625:683	The baseline color was measured according to the CIE L*a*b* system using a reflectance spectrophotometer.					
30970089	6	28	theme	red	766:768	arg1	wine					770:773	red wine	766:773	red wine	766:773	After these tests, the specimens were individually immersed in cola soft drink, red wine, or distilled water (n = 8) for 28 days.					
30970089	8	29	theme	aging	1000:1004	arg1	process					1006:1012	the aging process	996:1012	the aging process	996:1012	The color stability was determined using the difference between the coordinates obtained before and after the aging process.					
30970089	11	30	theme	roughness	1339:1347	arg1	values					1349:1354	the surface roughness values	1327:1354	the surface roughness values	1327:1354	The storage in red wine resulted in a higher variation in the surface roughness values, especially when only the nanodiamond was incorporated to the material (p < 0.05).					
30970089	2	31	theme	nanodiamond-modified	365:384	arg1	material					386:393	nanodiamond-modified material	365:393	nanodiamond-modified material	365:393	Four bisacrylic resins were evaluated, namely, control, chitosan-modified material, nanodiamond-modified material, and chitosan-nanodiamond-modified material.					
30970089	0	32	theme	surface	20:26	arg1	roughness					28:36	surface roughness	20:36	surface roughness	20:36	Color stability and surface roughness of chitosan- and nanodiamond-modified bisacrylic resin.					
30970089	4	33	theme	mm	576:577	arg1	cut-off					560:566	cut-off	560:566	cut-off	560:566	The surface roughness was determined using a profilometer with a cut-off of 0.25 mm.					
30970089	5	34	theme	L	633:633	arg1	*					638:638	the CIE L*a*b*	625:638	the CIE L*a*b* system using a reflectance spectrophotometer	625:683	The baseline color was measured according to the CIE L*a*b* system using a reflectance spectrophotometer.					
30970089	9	35	theme	=	1103:1103	arg1	α					1101:1101	α = 0.05	1101:1108	α = 0.05	1101:1108	The data on roughness and color change were evaluated using ANOVA and the Tukey test (α = 0.05).					
30970089	9	35	theme	=	1103:1103	arg1	test					1095:1098	the Tukey test	1085:1098	the Tukey test (α = 0.05)	1085:1109	The data on roughness and color change were evaluated using ANOVA and the Tukey test (α = 0.05).					
30970089	9	36	theme	color	1041:1045	arg1	change					1047:1052	color change	1041:1052	color change	1041:1052	The data on roughness and color change were evaluated using ANOVA and the Tukey test (α = 0.05).					
30970089	12	37	theme	nanodiamonds	1478:1489	arg1	incorporation					1443:1455	The incorporation	1439:1455	The incorporation of both chitosan and nanodiamonds	1439:1489	The incorporation of both chitosan and nanodiamonds are promising in providing an improvement in the properties of the bisacrylic resin when they are simultaneously incorporated in the product.					
30970089	12	37	theme	nanodiamonds	1478:1489	arg1	promising					1495:1503	promising	1495:1503	promising	1495:1503	The incorporation of both chitosan and nanodiamonds are promising in providing an improvement in the properties of the bisacrylic resin when they are simultaneously incorporated in the product.					
30970089	2	38	theme	bisacrylic	286:295	arg1	resins					297:302	Four bisacrylic resins	281:302	Four bisacrylic resins	281:302	Four bisacrylic resins were evaluated, namely, control, chitosan-modified material, nanodiamond-modified material, and chitosan-nanodiamond-modified material.					
30970089	10	39	theme	better	1216:1221	arg1	stability					1229:1237	a better color stability	1214:1237	a better color stability	1214:1237	The results show that the incorporation of nanodiamonds and chitosan into a bisacrylic resin provided a better color stability to the materials (p = 0.007).					
30970089	1	40	theme	color	190:194	arg1	stability					196:204	color stability	190:204	color stability	190:204	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	11	41	theme	surface	1331:1337	arg1	values					1349:1354	the surface roughness values	1327:1354	the surface roughness values	1327:1354	The storage in red wine resulted in a higher variation in the surface roughness values, especially when only the nanodiamond was incorporated to the material (p < 0.05).					
30970089	11	42	theme	p	1428:1428	arg1	material					1418:1425	the material	1414:1425	the material (p < 0.05)	1414:1436	The storage in red wine resulted in a higher variation in the surface roughness values, especially when only the nanodiamond was incorporated to the material (p < 0.05).					
30970089	11	42	theme	p	1428:1428	arg1	<					1430:1430	p < 0.05	1428:1435	p < 0.05	1428:1435	The storage in red wine resulted in a higher variation in the surface roughness values, especially when only the nanodiamond was incorporated to the material (p < 0.05).					
30970089	2	43	theme	chitosan-nanodiamond-modified	400:428	arg1	material					430:437	chitosan-nanodiamond-modified material	400:437	chitosan-nanodiamond-modified material	400:437	Four bisacrylic resins were evaluated, namely, control, chitosan-modified material, nanodiamond-modified material, and chitosan-nanodiamond-modified material.					
30970089	5	44	theme	reflectance	655:665	arg1	spectrophotometer					667:683	a reflectance spectrophotometer	653:683	a reflectance spectrophotometer	653:683	The baseline color was measured according to the CIE L*a*b* system using a reflectance spectrophotometer.					
30970089	6	45	theme	soft	754:757	arg1	drink					759:763	cola soft drink	749:763	cola soft drink	749:763	After these tests, the specimens were individually immersed in cola soft drink, red wine, or distilled water (n = 8) for 28 days.					
30970089	1	46	theme	study	110:114	arg1	aim					98:100	The aim	94:100	The aim of this study	94:114	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	0	47	theme	chitosan-	41:49	arg1	resin					87:91	chitosan- and nanodiamond-modified bisacrylic resin	41:91	chitosan- and nanodiamond-modified bisacrylic resin	41:91	Color stability and surface roughness of chitosan- and nanodiamond-modified bisacrylic resin.					
30970089	6	48	theme	cola	749:752	arg1	drink					759:763	cola soft drink	749:763	cola soft drink	749:763	After these tests, the specimens were individually immersed in cola soft drink, red wine, or distilled water (n = 8) for 28 days.					
30970089	4	49	with	profilometer	540:551	arg1	cut-off					560:566	cut-off	560:566	cut-off	560:566	The surface roughness was determined using a profilometer with a cut-off of 0.25 mm.					
30970089	1	50	dep	stability	196:204	arg1	the					186:188	the	186:188	the	186:188	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	5	51	theme	baseline	584:591	arg1	color					593:597	The baseline color	580:597	The baseline color	580:597	The baseline color was measured according to the CIE L*a*b* system using a reflectance spectrophotometer.					
30970089	12	52	theme	resin	1569:1573	arg1	properties					1540:1549	the properties	1536:1549	the properties of the bisacrylic resin	1536:1573	The incorporation of both chitosan and nanodiamonds are promising in providing an improvement in the properties of the bisacrylic resin when they are simultaneously incorporated in the product.					
30970089	1	53	theme	surface	210:216	arg1	roughness					218:226	surface roughness	210:226	surface roughness	210:226	The aim of this study is to evaluate the effect of chitosan or nanodiamond incorporation on the color stability and surface roughness of a bisacrylic resin subjected to artificial aging.					
30970089	6	54	theme	=	798:798	arg1	n					796:796	n = 8	796:800	n = 8	796:800	After these tests, the specimens were individually immersed in cola soft drink, red wine, or distilled water (n = 8) for 28 days.					
30970089	6	54	theme	=	798:798	arg1	water					789:793	distilled water	779:793	distilled water (n = 8)	779:801	After these tests, the specimens were individually immersed in cola soft drink, red wine, or distilled water (n = 8) for 28 days.					
30970089	10	55	theme	color	1223:1227	arg1	stability					1229:1237	a better color stability	1214:1237	a better color stability	1214:1237	The results show that the incorporation of nanodiamonds and chitosan into a bisacrylic resin provided a better color stability to the materials (p = 0.007).					
30970089	0	56	theme	bisacrylic	76:85	arg1	resin					87:91	chitosan- and nanodiamond-modified bisacrylic resin	41:91	chitosan- and nanodiamond-modified bisacrylic resin	41:91	Color stability and surface roughness of chitosan- and nanodiamond-modified bisacrylic resin.					
30970089	10	57	theme	nanodiamonds	1155:1166	arg1	incorporation					1138:1150	the incorporation	1134:1150	the incorporation of nanodiamonds and chitosan into a bisacrylic resin	1134:1203	The results show that the incorporation of nanodiamonds and chitosan into a bisacrylic resin provided a better color stability to the materials (p = 0.007).					
30970089	11	58	from	variation	1314:1322	arg1	values					1349:1354	the surface roughness values	1327:1354	the surface roughness values	1327:1354	The storage in red wine resulted in a higher variation in the surface roughness values, especially when only the nanodiamond was incorporated to the material (p < 0.05).					
30970089	9	59	theme	Tukey	1089:1093	arg1	α					1101:1101	α = 0.05	1101:1108	α = 0.05	1101:1108	The data on roughness and color change were evaluated using ANOVA and the Tukey test (α = 0.05).					
30970089	9	59	theme	Tukey	1089:1093	arg1	test					1095:1098	the Tukey test	1085:1098	the Tukey test (α = 0.05)	1085:1109	The data on roughness and color change were evaluated using ANOVA and the Tukey test (α = 0.05).					
30970089	7	60	theme	surface	837:843	arg1	roughness					845:853	the surface roughness	833:853	the surface roughness	833:853	After the aging, the surface roughness and final color were re-evaluated.					
30970089	0	61	theme	nanodiamond-modified	55:74	arg1	resin					87:91	chitosan- and nanodiamond-modified bisacrylic resin	41:91	chitosan- and nanodiamond-modified bisacrylic resin	41:91	Color stability and surface roughness of chitosan- and nanodiamond-modified bisacrylic resin.					
30970089	9	62	from	data	1019:1022	arg1	roughness					1027:1035	roughness	1027:1035	roughness	1027:1035	The data on roughness and color change were evaluated using ANOVA and the Tukey test (α = 0.05).					
30970089	9	62	from	data	1019:1022	arg1	change					1047:1052	color change	1041:1052	color change	1041:1052	The data on roughness and color change were evaluated using ANOVA and the Tukey test (α = 0.05).					
31470273	4	0	theme	affected	769:776	arg1	levels					804:809	affected the nutritional component levels	769:809	affected the nutritional component levels	769:809	Flowering and pod-fill periods were identified as the critical periods for irrigations and affected the nutritional component levels.					
31470273	2	1	theme	growth	355:360	arg1	stages					362:367	different physiological growth stages	331:367	different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods)	331:440	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	3	2	theme	different	532:540	arg1	stages					563:568	different physiological growth stages	532:568	different physiological growth stages instead of full irrigation treatment	532:605	The findings showed that one or two irrigations at different physiological growth stages instead of full irrigation treatment might be sufficient to improve the nutritional attributes of chickpea.					
31470273	2	3	dep	different	331:339	arg1	physiological					341:353	physiological	341:353	physiological	341:353	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	6	4	theme	farming	1164:1170	arg1	practices					1142:1150	irrigation practices	1131:1150	irrigation practices of chickpea farming	1131:1170	It was concluded based on present findings that physiological growth stages should be taken into consideration in irrigation practices of chickpea farming.					
31470273	4	5	theme	critical	732:739	arg1	periods					701:707	Flowering and pod-fill periods	678:707	Flowering and pod-fill periods	678:707	Flowering and pod-fill periods were identified as the critical periods for irrigations and affected the nutritional component levels.					
31470273	4	5	theme	critical	732:739	arg1	periods					741:747	the critical periods	728:747	the critical periods for irrigations and affected the nutritional component levels	728:809	Flowering and pod-fill periods were identified as the critical periods for irrigations and affected the nutritional component levels.					
31470273	0	6	theme	grain	107:111	arg1	composition					125:135	grain nutritional composition	107:135	grain nutritional composition	107:135	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.) change grain nutritional composition.					
31470273	0	7	from	stages	60:65	arg1	irrigations					14:24	Supplementary irrigations	0:24	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.)	0:98	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.) change grain nutritional composition.					
31470273	1	8	theme	important	163:171	arg1	legumes					173:179	the most important legumes	154:179	the most important legumes	154:179	Chickpea is one the most important legumes consumed all around the world because of high protein content.					
31470273	5	9	theme	protein	825:831	arg1	level					833:837	The greatest protein level	812:837	The greatest protein level (29.52%)	812:846	The greatest protein level (29.52%) was obtained from the samples irrigated before flowering while the greatest total starch (36.30%) was obtained from the samples irrigated at the beginning of flowering.					
31470273	5	9	theme	protein	825:831	arg1	%					845:845	29.52%	840:845	29.52%	840:845	The greatest protein level (29.52%) was obtained from the samples irrigated before flowering while the greatest total starch (36.30%) was obtained from the samples irrigated at the beginning of flowering.					
31470273	5	10	theme	greatest	915:922	arg1	starch					930:935	total starch	924:935	the greatest total starch (36.30%)	911:944	The greatest protein level (29.52%) was obtained from the samples irrigated before flowering while the greatest total starch (36.30%) was obtained from the samples irrigated at the beginning of flowering.					
31470273	5	10	theme	greatest	915:922	arg1	%					943:943	36.30%	938:943	36.30%	938:943	The greatest protein level (29.52%) was obtained from the samples irrigated before flowering while the greatest total starch (36.30%) was obtained from the samples irrigated at the beginning of flowering.					
31470273	2	11	theme	chickpea	471:478	arg1	attributes					457:466	nutritional attributes	445:466	nutritional attributes of chickpea	445:478	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	2	12	from	stages	362:367	arg1	effects					295:301	the effects	291:301	the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea	291:478	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	2	12	from	stages	362:367	arg1	treatments					317:326	irrigation treatments	306:326	irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods)	306:440	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	4	13	theme	component	794:802	arg1	levels					804:809	affected the nutritional component levels	769:809	affected the nutritional component levels	769:809	Flowering and pod-fill periods were identified as the critical periods for irrigations and affected the nutritional component levels.					
31470273	6	14	theme	physiological	1065:1077	arg1	stages					1086:1091	physiological growth stages	1065:1091	physiological growth stages	1065:1091	It was concluded based on present findings that physiological growth stages should be taken into consideration in irrigation practices of chickpea farming.					
31470273	2	15	theme	irrigation	306:315	arg1	treatments					317:326	irrigation treatments	306:326	irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods)	306:440	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	0	16	theme	Supplementary	0:12	arg1	irrigations					14:24	Supplementary irrigations	0:24	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.)	0:98	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.) change grain nutritional composition.					
31470273	0	17	dep	Cicer	80:84	arg1	L.					96:97	Cicer arietinum L.	80:97	Cicer arietinum L.	80:97	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.) change grain nutritional composition.					
31470273	4	18	theme	nutritional	782:792	arg1	component					794:802	the nutritional component	778:802	affected the nutritional component levels	769:809	Flowering and pod-fill periods were identified as the critical periods for irrigations and affected the nutritional component levels.					
31470273	4	19	theme	Flowering	678:686	arg1	periods					701:707	Flowering and pod-fill periods	678:707	Flowering and pod-fill periods	678:707	Flowering and pod-fill periods were identified as the critical periods for irrigations and affected the nutritional component levels.					
31470273	4	19	theme	Flowering	678:686	arg1	periods					741:747	the critical periods	728:747	the critical periods for irrigations and affected the nutritional component levels	728:809	Flowering and pod-fill periods were identified as the critical periods for irrigations and affected the nutritional component levels.					
31470273	0	20	theme	different	29:37	arg1	stages					60:65	different physiological growth stages	29:65	different physiological growth stages of chickpea (Cicer arietinum L.)	29:98	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.) change grain nutritional composition.					
31470273	3	21	theme	growth	556:561	arg1	stages					563:568	different physiological growth stages	532:568	different physiological growth stages instead of full irrigation treatment	532:605	The findings showed that one or two irrigations at different physiological growth stages instead of full irrigation treatment might be sufficient to improve the nutritional attributes of chickpea.					
31470273	5	22	theme	flowering	1006:1014	arg1	beginning					993:1001	the beginning	989:1001	the beginning of flowering	989:1014	The greatest protein level (29.52%) was obtained from the samples irrigated before flowering while the greatest total starch (36.30%) was obtained from the samples irrigated at the beginning of flowering.					
31470273	6	23	theme	present	1043:1049	arg1	findings					1051:1058	present findings	1043:1058	present findings	1043:1058	It was concluded based on present findings that physiological growth stages should be taken into consideration in irrigation practices of chickpea farming.					
31470273	2	24	theme	pod-fill	424:431	arg1	periods					433:439	pod-fill periods	424:439	pod-fill periods	424:439	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	0	25	theme	nutritional	113:123	arg1	composition					125:135	grain nutritional composition	107:135	grain nutritional composition	107:135	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.) change grain nutritional composition.					
31470273	1	26	dep	one	150:152	arg1	consumed					181:188	consumed	181:188	consumed all around the world because of high protein content	181:241	Chickpea is one the most important legumes consumed all around the world because of high protein content.					
31470273	6	27	theme	chickpea	1155:1162	arg1	farming					1164:1170	chickpea farming	1155:1170	chickpea farming	1155:1170	It was concluded based on present findings that physiological growth stages should be taken into consideration in irrigation practices of chickpea farming.					
31470273	0	28	theme	growth	53:58	arg1	stages					60:65	different physiological growth stages	29:65	different physiological growth stages of chickpea (Cicer arietinum L.)	29:98	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.) change grain nutritional composition.					
31470273	3	29	theme	treatment	597:605	arg1	stages					563:568	different physiological growth stages	532:568	different physiological growth stages instead of full irrigation treatment	532:605	The findings showed that one or two irrigations at different physiological growth stages instead of full irrigation treatment might be sufficient to improve the nutritional attributes of chickpea.					
31470273	6	30	theme	growth	1079:1084	arg1	stages					1086:1091	physiological growth stages	1065:1091	physiological growth stages	1065:1091	It was concluded based on present findings that physiological growth stages should be taken into consideration in irrigation practices of chickpea farming.					
31470273	2	31	theme	flowering	401:409	arg1	beginning					388:396	beginning	388:396	beginning of flowering	388:409	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	2	31	theme	flowering	401:409	arg1	flowering					377:385	flowering	377:385	flowering	377:385	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	2	31	theme	flowering	401:409	arg1	pod-set					412:418	pod-set	412:418	pod-set	412:418	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	2	31	theme	flowering	401:409	arg1	periods					433:439	pod-fill periods	424:439	pod-fill periods	424:439	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	5	32	theme	greatest	816:823	arg1	level					833:837	The greatest protein level	812:837	The greatest protein level (29.52%)	812:846	The greatest protein level (29.52%) was obtained from the samples irrigated before flowering while the greatest total starch (36.30%) was obtained from the samples irrigated at the beginning of flowering.					
31470273	5	32	theme	greatest	816:823	arg1	%					845:845	29.52%	840:845	29.52%	840:845	The greatest protein level (29.52%) was obtained from the samples irrigated before flowering while the greatest total starch (36.30%) was obtained from the samples irrigated at the beginning of flowering.					
31470273	3	33	theme	nutritional	642:652	arg1	attributes					654:663	the nutritional attributes	638:663	the nutritional attributes of chickpea	638:675	The findings showed that one or two irrigations at different physiological growth stages instead of full irrigation treatment might be sufficient to improve the nutritional attributes of chickpea.					
31470273	3	34	theme	full	581:584	arg1	treatment					597:605	full irrigation treatment	581:605	full irrigation treatment	581:605	The findings showed that one or two irrigations at different physiological growth stages instead of full irrigation treatment might be sufficient to improve the nutritional attributes of chickpea.					
31470273	5	35	theme	total	924:928	arg1	starch					930:935	total starch	924:935	the greatest total starch (36.30%)	911:944	The greatest protein level (29.52%) was obtained from the samples irrigated before flowering while the greatest total starch (36.30%) was obtained from the samples irrigated at the beginning of flowering.					
31470273	5	35	theme	total	924:928	arg1	%					943:943	36.30%	938:943	36.30%	938:943	The greatest protein level (29.52%) was obtained from the samples irrigated before flowering while the greatest total starch (36.30%) was obtained from the samples irrigated at the beginning of flowering.					
31470273	2	36	theme	nutritional	445:455	arg1	attributes					457:466	nutritional attributes	445:466	nutritional attributes of chickpea	445:478	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	3	37	theme	irrigation	586:595	arg1	treatment					597:605	full irrigation treatment	581:605	full irrigation treatment	581:605	The findings showed that one or two irrigations at different physiological growth stages instead of full irrigation treatment might be sufficient to improve the nutritional attributes of chickpea.					
31470273	2	38	theme	different	331:339	arg1	stages					362:367	different physiological growth stages	331:367	different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods)	331:440	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	0	39	dep	different	29:37	arg1	physiological					39:51	physiological	39:51	physiological	39:51	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.) change grain nutritional composition.					
31470273	3	40	dep	different	532:540	arg1	physiological					542:554	physiological	542:554	physiological	542:554	The findings showed that one or two irrigations at different physiological growth stages instead of full irrigation treatment might be sufficient to improve the nutritional attributes of chickpea.					
31470273	3	41	from	stages	563:568	arg1	irrigations					517:527	one or two irrigations	506:527	one or two irrigations at different physiological growth stages instead of full irrigation treatment	506:605	The findings showed that one or two irrigations at different physiological growth stages instead of full irrigation treatment might be sufficient to improve the nutritional attributes of chickpea.					
31470273	3	42	theme	chickpea	668:675	arg1	attributes					654:663	the nutritional attributes	638:663	the nutritional attributes of chickpea	638:675	The findings showed that one or two irrigations at different physiological growth stages instead of full irrigation treatment might be sufficient to improve the nutritional attributes of chickpea.					
31470273	2	43	theme	current	248:254	arg1	study					256:260	The current study	244:260	The current study	244:260	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	2	44	theme	treatments	317:326	arg1	effects					295:301	the effects	291:301	the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea	291:478	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	6	45	theme	irrigation	1131:1140	arg1	practices					1142:1150	irrigation practices	1131:1150	irrigation practices of chickpea farming	1131:1170	It was concluded based on present findings that physiological growth stages should be taken into consideration in irrigation practices of chickpea farming.					
31470273	1	46	theme	high	222:225	arg1	content					235:241	high protein content	222:241	high protein content	222:241	Chickpea is one the most important legumes consumed all around the world because of high protein content.					
31470273	1	47	theme	protein	227:233	arg1	content					235:241	high protein content	222:241	high protein content	222:241	Chickpea is one the most important legumes consumed all around the world because of high protein content.					
31470273	0	48	theme	chickpea	70:77	arg1	stages					60:65	different physiological growth stages	29:65	different physiological growth stages of chickpea (Cicer arietinum L.)	29:98	Supplementary irrigations at different physiological growth stages of chickpea (Cicer arietinum L.) change grain nutritional composition.					
31470273	4	49	theme	pod-fill	692:699	arg1	periods					701:707	Flowering and pod-fill periods	678:707	Flowering and pod-fill periods	678:707	Flowering and pod-fill periods were identified as the critical periods for irrigations and affected the nutritional component levels.					
31470273	4	49	theme	pod-fill	692:699	arg1	periods					741:747	the critical periods	728:747	the critical periods for irrigations and affected the nutritional component levels	728:809	Flowering and pod-fill periods were identified as the critical periods for irrigations and affected the nutritional component levels.					
31470273	2	50	from	effects	295:301	arg1	stages					362:367	different physiological growth stages	331:367	different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods)	331:440	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
31470273	2	50	from	effects	295:301	arg1	attributes					457:466	nutritional attributes	445:466	nutritional attributes of chickpea	445:478	The current study was conducted to investigate the effects of irrigation treatments at different physiological growth stages (before flowering, beginning of flowering, pod-set and pod-fill periods) on nutritional attributes of chickpea.					
30310280	11	0	theme	re-epithelialized	1624:1640	arg1	composition					1662:1672	the re-epithelialized and neovascularized composition	1620:1672	the re-epithelialized and neovascularized composition	1620:1672	Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition.					
30310280	9	1	contain	had	1250:1252	arg2	effects					1257:1263	no effects	1254:1263	no effects on the metabolism and proliferation of keratinocytes	1254:1316	CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes.					
30310280	9	1	contain	had	1250:1252	arg1	NP					1215:1216	CMCSOH30 NP	1206:1216	CMCSOH30 NP	1206:1216	CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes.					
30310280	6	2	theme	conventional	942:953	arg1	methods					955:961	conventional methods	942:961	conventional methods	942:961	Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods.					
30310280	4	3	theme	mild	626:629	arg1	method					646:651	mild ionic gelation method	626:651	mild ionic gelation method	626:651	METHODS CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering.					
30310280	3	4	from	effects	547:553	arg1	healing					564:570	wound healing	558:570	wound healing	558:570	PURPOSE To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.					
30310280	9	5	from	effects	1257:1263	arg1	proliferation					1287:1299	proliferation	1287:1299	proliferation	1287:1299	CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes.					
30310280	9	5	from	effects	1257:1263	arg1	metabolism					1272:1281	metabolism	1272:1281	metabolism	1272:1281	CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes.					
30310280	4	6	theme	NP	694:695	arg1	properties					657:666	properties	657:666	properties of the prepared CMCS-OH30 NP	657:695	METHODS CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering.					
30310280	6	7	theme	NP	920:921	arg1	migration					877:885	cell migration	872:885	cell migration	872:885	Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods.					
30310280	6	7	theme	NP	920:921	arg1	uptake					900:905	cellular uptake	891:905	cellular uptake	891:905	Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods.					
30310280	6	7	theme	NP	920:921	arg1	Cytotoxicity					858:869	Cytotoxicity	858:869	Cytotoxicity	858:869	Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods.					
30310280	13	8	theme	better	1914:1919	arg1	prognosis					1921:1929	better prognosis	1914:1929	better prognosis	1914:1929	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	10	9	theme	OH30	1458:1461	arg1	administration					1463:1476	OH30 administration	1458:1476	OH30 administration alone	1458:1482	In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone.					
30310280	12	10	theme	anti-inflammatory	1705:1721	arg1	IL10					1732:1735	a steady anti-inflammatory cytokine IL10	1696:1735	a steady anti-inflammatory cytokine IL10 expression	1696:1746	CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.					
30310280	5	11	theme	Encapsulation	742:754	arg1	efficiency					756:765	Encapsulation efficiency	742:765	Encapsulation efficiency	742:765	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	12	12	theme	IL10	1732:1735	arg1	expression					1737:1746	a steady anti-inflammatory cytokine IL10 expression	1696:1746	a steady anti-inflammatory cytokine IL10 expression	1696:1746	CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.					
30310280	4	13	theme	prepared	675:682	arg1	NP					694:695	the prepared CMCS-OH30 NP	671:695	the prepared CMCS-OH30 NP	671:695	METHODS CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering.					
30310280	7	14	theme	animal	1066:1071	arg1	models					1073:1078	full-thickness excision animal models	1042:1078	full-thickness excision animal models	1042:1078	The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.					
30310280	7	15	from	effects	968:974	arg1	healing					1014:1020	the wound healing	1004:1020	the wound healing	1004:1020	The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.					
30310280	2	16	theme	king	228:231	arg1	cobra					233:237	king cobra	228:237	king cobra	228:237	Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.					
30310280	7	17	theme	full-thickness	1042:1055	arg1	models					1073:1078	full-thickness excision animal models	1042:1078	full-thickness excision animal models	1042:1078	The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.					
30310280	2	18	theme	innate	268:273	arg1	immunity					275:282	the innate immunity	264:282	the innate immunity	264:282	Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.					
30310280	1	19	theme	desirable	146:154	arg1	healing					133:139	BACKGROUND Nonscar wound healing	108:139	BACKGROUND Nonscar wound healing	108:139	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	1	19	theme	desirable	146:154	arg1	treatment					156:164	a desirable treatment	144:164	a desirable treatment for cutaneous wounds worldwide	144:195	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	8	20	from	NP	1146:1147	arg1	release					1093:1099	The release	1089:1099	The release of encapsulated OH30 from prepared CMCS-OH30 NP	1089:1147	RESULTS The release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner.					
30310280	4	21	theme	dynamic	716:722	arg1	scattering					730:739	dynamic light scattering	716:739	dynamic light scattering	716:739	METHODS CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering.					
30310280	5	22	theme	OH30	801:804	arg1	stability					768:776	stability	768:776	stability	768:776	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	5	22	theme	OH30	801:804	arg1	profile					790:796	release profile	782:796	release profile	782:796	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	5	22	theme	OH30	801:804	arg1	efficiency					756:765	Encapsulation efficiency	742:765	Encapsulation efficiency	742:765	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	13	23	theme	biodegradable	1845:1857	arg1	system					1873:1878	The prepared biodegradable drug delivery system	1832:1878	The prepared biodegradable drug delivery system	1832:1878	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	0	24	theme	wound	93:97	arg1	healing					99:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.					
30310280	1	25	theme	wounds	180:185	arg1	worldwide					187:195	cutaneous wounds worldwide	170:195	cutaneous wounds worldwide	170:195	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	13	26	theme	delivery	1864:1871	arg1	system					1873:1878	The prepared biodegradable drug delivery system	1832:1878	The prepared biodegradable drug delivery system	1832:1878	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	11	27	theme	granulation	1583:1593	arg1	formation					1602:1610	the granulation tissue formation	1579:1610	the granulation tissue formation	1579:1610	Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition.					
30310280	7	28	theme	NP	998:999	arg1	effects					968:974	The effects	964:974	The effects of prepared CMCS-OH30 NP on the wound healing	964:1020	The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.					
30310280	12	29	theme	several	1785:1791	arg1	cytokines					1810:1818	several pro-inflammatory cytokines	1785:1818	several pro-inflammatory cytokines	1785:1818	CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.					
30310280	0	30	theme	bioactive	49:57	arg1	healing					99:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.					
30310280	7	31	theme	prepared	979:986	arg1	NP					998:999	prepared CMCS-OH30 NP	979:999	prepared CMCS-OH30 NP	979:999	The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.					
30310280	13	32	attach	released	1971:1978	arg2	effects					1955:1961	the combined effects	1942:1961	the combined effects of OH30 released from the nanoparticles	1942:2001	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	13	32	attach	released	1971:1978	arg1	nanoparticles					1989:2001	the nanoparticles	1985:2001	the nanoparticles	1985:2001	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	12	33	theme	cytokines	1810:1818	arg1	expressions					1770:1780	the expressions	1766:1780	the expressions of several pro-inflammatory cytokines	1766:1818	CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.					
30310280	13	34	theme	combined	1946:1953	arg1	effects					1955:1961	the combined effects	1942:1961	the combined effects of OH30 released from the nanoparticles	1942:2001	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	11	35	theme	Histopathological	1485:1501	arg1	examination					1503:1513	Histopathological examination	1485:1513	Histopathological examination	1485:1513	Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition.					
30310280	0	36	theme	Carboxymethyl	0:12	arg1	chitosan					14:21	Carboxymethyl chitosan	0:21	Carboxymethyl chitosan	0:21	Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.					
30310280	8	37	theme	OH30	1117:1120	arg1	release					1093:1099	The release	1089:1099	The release of encapsulated OH30 from prepared CMCS-OH30 NP	1089:1147	RESULTS The release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner.					
30310280	1	38	theme	BACKGROUND	108:117	arg1	healing					133:139	BACKGROUND Nonscar wound healing	108:139	BACKGROUND Nonscar wound healing	108:139	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	1	38	theme	BACKGROUND	108:117	arg1	treatment					156:164	a desirable treatment	144:164	a desirable treatment for cutaneous wounds worldwide	144:195	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	3	39	theme	enzymatic	401:409	arg1	digestion					411:419	the enzymatic digestion	397:419	the enzymatic digestion	397:419	PURPOSE To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.					
30310280	8	40	theme	prepared	1127:1134	arg1	NP					1146:1147	prepared CMCS-OH30 NP	1127:1147	prepared CMCS-OH30 NP	1127:1147	RESULTS The release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner.					
30310280	1	41	theme	wound	127:131	arg1	healing					133:139	BACKGROUND Nonscar wound healing	108:139	BACKGROUND Nonscar wound healing	108:139	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	1	41	theme	wound	127:131	arg1	treatment					156:164	a desirable treatment	144:164	a desirable treatment for cutaneous wounds worldwide	144:195	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	3	42	theme	OH30	444:447	arg1	release					433:439	release	433:439	release of OH30	433:447	PURPOSE To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.					
30310280	2	43	theme	Peptide	198:204	arg1	OH30					217:220	OH30	217:220	OH30	217:220	Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.					
30310280	2	43	theme	Peptide	198:204	arg1	OH-CATH30					206:214	Peptide OH-CATH30	198:214	Peptide OH-CATH30 (OH30) from king cobra	198:237	Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.					
30310280	10	44	theme	excision	1341:1348	arg1	models					1357:1362	the full-thickness excision animal models	1322:1362	the full-thickness excision animal models	1322:1362	In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone.					
30310280	0	45	theme	benefit	77:83	arg1	healing					99:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.					
30310280	10	46	theme	NP	1379:1380	arg1	treatment					1382:1390	the CMCS-OH30 NP treatment	1365:1390	the CMCS-OH30 NP treatment	1365:1390	In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone.					
30310280	0	47	theme	peptide	59:65	arg1	healing					99:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.					
30310280	2	48	theme	wound	362:366	arg1	healing					368:374	nonscar wound healing	354:374	nonscar wound healing	354:374	Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.					
30310280	6	49	theme	cell	872:875	arg1	migration					877:885	cell migration	872:885	cell migration	872:885	Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods.					
30310280	0	50	dep	nanoparticles	23:35	arg1	loaded					37:42	loaded	37:42	nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing	23:105	Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.					
30310280	9	51	dep	metabolism	1272:1281	arg1	the					1268:1270	the	1268:1270	the	1268:1270	CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes.					
30310280	3	52	from	encapsulated	455:466	arg1	chitosan					485:492	carboxymethyl chitosan	471:492	carboxymethyl chitosan	471:492	PURPOSE To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.					
30310280	9	53	theme	CMCSOH30	1206:1213	arg1	NP					1215:1216	CMCSOH30 NP	1206:1216	CMCSOH30 NP	1206:1216	CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes.					
30310280	2	54	theme	anti-inflammatory	298:314	arg1	micro-environment					316:332	an anti-inflammatory micro-environment	295:332	an anti-inflammatory micro-environment which might benefit nonscar wound healing	295:374	Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.					
30310280	8	55	theme	controlled	1187:1196	arg1	manner					1198:1203	a controlled manner	1185:1203	a controlled manner	1185:1203	RESULTS The release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner.					
30310280	8	56	dep	RESULTS	1081:1087	arg1	maintained					1153:1162	maintained	1153:1162	was maintained for at least 24 h in a controlled manner	1149:1203	RESULTS The release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner.					
30310280	9	57	theme	cell	1231:1234	arg1	migration					1236:1244	the cell migration	1227:1244	the cell migration	1227:1244	CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes.					
30310280	3	58	theme	wound	558:562	arg1	healing					564:570	wound healing	558:570	wound healing	558:570	PURPOSE To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.					
30310280	4	59	theme	CMCS-OH30	684:692	arg1	NP					694:695	the prepared CMCS-OH30 NP	671:695	the prepared CMCS-OH30 NP	671:695	METHODS CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering.					
30310280	5	60	theme	prepared	811:818	arg1	NP					830:831	prepared CMCS-OH30 NP	811:831	prepared CMCS-OH30 NP	811:831	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	12	61	theme	cytokine	1723:1730	arg1	IL10					1732:1735	a steady anti-inflammatory cytokine IL10	1696:1735	a steady anti-inflammatory cytokine IL10 expression	1696:1746	CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.					
30310280	11	62	theme	neovascularized	1646:1660	arg1	composition					1662:1672	the re-epithelialized and neovascularized composition	1620:1672	the re-epithelialized and neovascularized composition	1620:1672	Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition.					
30310280	13	63	dep	CONCLUSION	1821:1830	arg1	accelerates					1880:1890	accelerates	1880:1890	accelerates the healing	1880:1902	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	13	63	dep	CONCLUSION	1821:1830	arg1	shows					1908:1912	shows	1908:1912	shows better prognosis because of the combined effects of OH30 released from the nanoparticles	1908:2001	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	12	64	theme	steady	1698:1703	arg1	IL10					1732:1735	a steady anti-inflammatory cytokine IL10	1696:1735	a steady anti-inflammatory cytokine IL10 expression	1696:1746	CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.					
30310280	7	65	theme	excision	1057:1064	arg1	models					1073:1078	full-thickness excision animal models	1042:1078	full-thickness excision animal models	1042:1078	The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.					
30310280	11	66	theme	wound	1552:1556	arg1	healing					1558:1564	wound healing	1552:1564	wound healing	1552:1564	Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition.					
30310280	0	67	theme	nonscar	85:91	arg1	healing					99:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.					
30310280	9	68	theme	keratinocytes	1304:1316	arg1	proliferation					1287:1299	proliferation	1287:1299	proliferation	1287:1299	CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes.					
30310280	9	68	theme	keratinocytes	1304:1316	arg1	metabolism					1272:1281	metabolism	1272:1281	metabolism	1272:1281	CMCSOH30 NP enhanced the cell migration but had no effects on the metabolism and proliferation of keratinocytes.					
30310280	11	69	theme	tissue	1595:1600	arg1	formation					1602:1610	the granulation tissue formation	1579:1610	the granulation tissue formation	1579:1610	Histopathological examination suggested that CMCS-OH30 NP promoted wound healing by enhancing the granulation tissue formation through the re-epithelialized and neovascularized composition.					
30310280	1	70	theme	cutaneous	170:178	arg1	wounds					180:185	cutaneous wounds	170:185	cutaneous wounds worldwide	170:195	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	13	71	theme	drug	1859:1862	arg1	system					1873:1878	The prepared biodegradable drug delivery system	1832:1878	The prepared biodegradable drug delivery system	1832:1878	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	4	72	theme	light	724:728	arg1	scattering					730:739	dynamic light scattering	716:739	dynamic light scattering	716:739	METHODS CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering.					
30310280	5	73	from	NP	830:831	arg1	stability					768:776	stability	768:776	stability	768:776	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	5	73	from	NP	830:831	arg1	profile					790:796	release profile	782:796	release profile	782:796	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	5	73	from	NP	830:831	arg1	efficiency					756:765	Encapsulation efficiency	742:765	Encapsulation efficiency	742:765	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	7	74	theme	wound	1008:1012	arg1	healing					1014:1020	the wound healing	1004:1020	the wound healing	1004:1020	The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.					
30310280	10	75	theme	full-thickness	1326:1339	arg1	models					1357:1362	the full-thickness excision animal models	1322:1362	the full-thickness excision animal models	1322:1362	In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone.					
30310280	5	76	theme	release	782:788	arg1	profile					790:796	release profile	782:796	release profile	782:796	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	2	77	from	cobra	233:237	arg1	OH30					217:220	OH30	217:220	OH30	217:220	Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.					
30310280	2	77	from	cobra	233:237	arg1	OH-CATH30					206:214	Peptide OH-CATH30	198:214	Peptide OH-CATH30 (OH30) from king cobra	198:237	Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.					
30310280	8	78	theme	encapsulated	1104:1115	arg1	OH30					1117:1120	encapsulated OH30	1104:1120	encapsulated OH30	1104:1120	RESULTS The release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner.					
30310280	4	79	theme	ionic	631:635	arg1	method					646:651	mild ionic gelation method	626:651	mild ionic gelation method	626:651	METHODS CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering.					
30310280	3	80	theme	carboxymethyl	471:483	arg1	chitosan					485:492	carboxymethyl chitosan	471:492	carboxymethyl chitosan	471:492	PURPOSE To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.					
30310280	7	81	theme	CMCS-OH30	988:996	arg1	NP					998:999	prepared CMCS-OH30 NP	979:999	prepared CMCS-OH30 NP	979:999	The effects of prepared CMCS-OH30 NP on the wound healing was investigated by full-thickness excision animal models.					
30310280	12	82	theme	pro-inflammatory	1793:1808	arg1	cytokines					1810:1818	several pro-inflammatory cytokines	1785:1818	several pro-inflammatory cytokines	1785:1818	CMCS-OH30 NP induced a steady anti-inflammatory cytokine IL10 expression but downregulated the expressions of several pro-inflammatory cytokines.					
30310280	4	83	theme	METHODS	588:594	arg1	NP					606:607	METHODS CMCS-OH30 NP	588:607	METHODS CMCS-OH30 NP	588:607	METHODS CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering.					
30310280	13	84	theme	prepared	1836:1843	arg1	system					1873:1878	The prepared biodegradable drug delivery system	1832:1878	The prepared biodegradable drug delivery system	1832:1878	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30310280	3	85	theme	encapsulated	455:466	arg1	nanoparticles					494:506	OH30 encapsulated in carboxymethyl chitosan nanoparticles	450:506	OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP)	450:521	PURPOSE To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.					
30310280	3	85	theme	encapsulated	455:466	arg1	NP					519:520	CMCS-OH30 NP	509:520	CMCS-OH30 NP	509:520	PURPOSE To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.					
30310280	8	86	theme	CMCS-OH30	1136:1144	arg1	NP					1146:1147	prepared CMCS-OH30 NP	1127:1147	prepared CMCS-OH30 NP	1127:1147	RESULTS The release of encapsulated OH30 from prepared CMCS-OH30 NP was maintained for at least 24 h in a controlled manner.					
30310280	1	87	theme	Nonscar	119:125	arg1	healing					133:139	BACKGROUND Nonscar wound healing	108:139	BACKGROUND Nonscar wound healing	108:139	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	1	87	theme	Nonscar	119:125	arg1	treatment					156:164	a desirable treatment	144:164	a desirable treatment for cutaneous wounds worldwide	144:195	BACKGROUND Nonscar wound healing is a desirable treatment for cutaneous wounds worldwide.					
30310280	10	88	theme	animal	1350:1355	arg1	models					1357:1362	the full-thickness excision animal models	1322:1362	the full-thickness excision animal models	1322:1362	In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone.					
30310280	5	89	theme	CMCS-OH30	820:828	arg1	NP					830:831	prepared CMCS-OH30 NP	811:831	prepared CMCS-OH30 NP	811:831	Encapsulation efficiency, stability and release profile of OH30 from prepared CMCS-OH30 NP were determined by HPLC.					
30310280	3	90	from	chitosan	485:492	arg1	encapsulated					455:466	encapsulated	455:466	encapsulated	455:466	PURPOSE To overcome the enzymatic digestion and control release of OH30, OH30 encapsulated in carboxymethyl chitosan nanoparticles (CMCS-OH30 NP) were prepared and their effects on wound healing were evaluated.					
30310280	0	91	theme	OH-CATH30	67:75	arg1	healing					99:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	bioactive peptide OH-CATH30 benefit nonscar wound healing	49:105	Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing.					
30310280	6	92	theme	cellular	891:898	arg1	uptake					900:905	cellular uptake	891:905	cellular uptake	891:905	Cytotoxicity, cell migration and cellular uptake of CMCS-OH30 NP were determined by conventional methods.					
30310280	4	93	theme	gelation	637:644	arg1	method					646:651	mild ionic gelation method	626:651	mild ionic gelation method	626:651	METHODS CMCS-OH30 NP were prepared by mild ionic gelation method and properties of the prepared CMCS-OH30 NP were determined by dynamic light scattering.					
30310280	10	94	theme	wound	1422:1426	arg1	healing					1428:1434	the wound healing	1418:1434	the wound healing	1418:1434	In the full-thickness excision animal models, the CMCS-OH30 NP treatment significantly accelerated the wound healing compared with CMCS or OH30 administration alone.					
30310280	2	95	theme	nonscar	354:360	arg1	healing					368:374	nonscar wound healing	354:374	nonscar wound healing	354:374	Peptide OH-CATH30 (OH30) from king cobra can selectively regulate the innate immunity and create an anti-inflammatory micro-environment which might benefit nonscar wound healing.					
30310280	13	96	theme	OH30	1966:1969	arg1	effects					1955:1961	the combined effects	1942:1961	the combined effects of OH30 released from the nanoparticles	1942:2001	CONCLUSION The prepared biodegradable drug delivery system accelerates the healing and shows better prognosis because of the combined effects of OH30 released from the nanoparticles.					
30143668	0	0	theme	starch	75:80	arg1	synthesis					82:90	starch synthesis	75:90	starch synthesis	75:90	Evolutionary, structural and expression analysis of core genes involved in starch synthesis.					
30143668	8	1	theme	functional	1631:1640	arg1	divergence					1655:1664	the functional evolutionary divergence	1627:1664	the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants	1627:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	7	2	theme	Expression	1234:1243	arg1	profiling					1245:1253	Expression profiling	1234:1253	Expression profiling	1234:1253	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	8	3	theme	future	1551:1556	arg1	studies					1558:1564	future studies	1551:1564	future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants	1551:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	8	4	from	understanding	1586:1598	arg1	plants					1697:1702	plants	1697:1702	plants	1697:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	6	5	theme	amino	1102:1106	arg1	acids					1108:1112	amino acids	1102:1112	amino acids	1102:1112	A structure analysis of these proteins suggested that the deletion/mutation of amino acids in some active sites resulted in not only structural variation but also sub-functionalization or neo-functionalization.					
30143668	5	6	theme	complicated	879:889	arg1	processes					904:912	complicated evolutionary processes	879:912	complicated evolutionary processes	879:912	The results showed that AGPase, SS, SBE and DBE have undergone complicated evolutionary processes in plants and that gene/genome duplications are responsible for the observed differences in isoform numbers.					
30143668	4	7	from	patterns	703:710	arg1	biosynthesis					747:758	starch biosynthesis	740:758	starch biosynthesis	740:758	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	7	8	theme	starch	1422:1427	arg1	biosynthesis					1429:1440	starch biosynthesis	1422:1440	starch biosynthesis	1422:1440	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	4	9	dep	structures	677:686	arg1	the					665:667	the	665:667	the	665:667	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	6	10	theme	active	1122:1127	arg1	sites					1129:1133	some active sites	1117:1133	some active sites	1117:1133	A structure analysis of these proteins suggested that the deletion/mutation of amino acids in some active sites resulted in not only structural variation but also sub-functionalization or neo-functionalization.					
30143668	8	11	theme	SS	1677:1678	arg1	understanding					1586:1598	understanding	1586:1598	understanding of starch biosynthesis	1586:1621	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	8	11	theme	SS	1677:1678	arg1	divergence					1655:1664	the functional evolutionary divergence	1627:1664	the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants	1627:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	6	12	theme	acids	1108:1112	arg1	deletion/mutation					1081:1097	the deletion/mutation	1077:1097	the deletion/mutation of amino acids in some active sites	1077:1133	A structure analysis of these proteins suggested that the deletion/mutation of amino acids in some active sites resulted in not only structural variation but also sub-functionalization or neo-functionalization.					
30143668	8	13	theme	biosynthetic	1499:1510	arg1	pathway					1512:1518	the starch biosynthetic pathway	1488:1518	the starch biosynthetic pathway	1488:1518	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	4	14	theme	genes	731:735	arg1	patterns					703:710	expression patterns	692:710	expression patterns	692:710	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	4	14	theme	genes	731:735	arg1	structures					677:686	protein structures	669:686	protein structures	669:686	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	1	15	theme	important	150:158	arg1	resource					168:175	an important natural resource	147:175	an important natural resource for food, feed and industrial raw materials	147:219	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	4	16	theme	functional	792:801	arg1	differences					803:813	their functional differences	786:813	their functional differences	786:813	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	3	17	theme	ADP-glucose	521:531	arg1	pyrophosphorylase					533:549	ADP-glucose pyrophosphorylase	521:549	ADP-glucose pyrophosphorylase (AGPase)	521:558	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	3	17	theme	ADP-glucose	521:531	arg1	AGPase					552:557	AGPase	552:557	AGPase	552:557	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	6	18	theme	proteins	1053:1060	arg1	analysis					1035:1042	A structure analysis	1023:1042	A structure analysis of these proteins	1023:1060	A structure analysis of these proteins suggested that the deletion/mutation of amino acids in some active sites resulted in not only structural variation but also sub-functionalization or neo-functionalization.					
30143668	1	19	theme	natural	160:166	arg1	resource					168:175	an important natural resource	147:175	an important natural resource for food, feed and industrial raw materials	147:219	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	1	20	from	carbohydrate	120:131	arg1	plants					136:141	plants	136:141	plants	136:141	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	4	21	theme	expression	692:701	arg1	patterns					703:710	expression patterns	692:710	expression patterns	692:710	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	3	22	theme	comprehensive	397:409	arg1	analysis					424:431	a comprehensive phylogenetic analysis	395:431	a comprehensive phylogenetic analysis of 74 sequenced plant genomes	395:461	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	5	23	theme	gene/genome	933:943	arg1	duplications					945:956	gene/genome duplications	933:956	gene/genome duplications	933:956	The results showed that AGPase, SS, SBE and DBE have undergone complicated evolutionary processes in plants and that gene/genome duplications are responsible for the observed differences in isoform numbers.					
30143668	8	24	theme	pathway	1512:1518	arg1	atlas					1479:1483	a comprehensive atlas	1463:1483	a comprehensive atlas of the starch biosynthetic pathway	1463:1518	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	3	25	theme	starch	561:566	arg1	SS					578:579	SS	578:579	SS	578:579	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	3	25	theme	starch	561:566	arg1	synthase					568:575	starch synthase	561:575	starch synthase (SS)	561:580	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	5	26	from	differences	991:1001	arg1	numbers					1014:1020	isoform numbers	1006:1020	isoform numbers	1006:1020	The results showed that AGPase, SS, SBE and DBE have undergone complicated evolutionary processes in plants and that gene/genome duplications are responsible for the observed differences in isoform numbers.					
30143668	3	27	theme	phylogenetic	411:422	arg1	analysis					424:431	a comprehensive phylogenetic analysis	395:431	a comprehensive phylogenetic analysis of 74 sequenced plant genomes	395:461	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	7	28	theme	related	1368:1374	arg1	patterns					1359:1366	spatio-temporally divergent expression patterns	1320:1366	spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis	1320:1440	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	7	29	from	composition	1383:1393	arg1	biosynthesis					1429:1440	starch biosynthesis	1422:1440	starch biosynthesis	1422:1440	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	3	30	theme	plant	449:453	arg1	genomes					455:461	74 sequenced plant genomes	436:461	74 sequenced plant genomes	436:461	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	5	31	theme	evolutionary	891:902	arg1	processes					904:912	complicated evolutionary processes	879:912	complicated evolutionary processes	879:912	The results showed that AGPase, SS, SBE and DBE have undergone complicated evolutionary processes in plants and that gene/genome duplications are responsible for the observed differences in isoform numbers.					
30143668	8	32	from	divergence	1655:1664	arg1	plants					1697:1702	plants	1697:1702	plants	1697:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	2	33	theme	starch	269:274	arg1	biosynthesis					276:287	starch biosynthesis	269:287	starch biosynthesis	269:287	However, the details regarding the pathway for starch biosynthesis and the diversity of biosynthetic enzymes involved in this process are poorly understood.					
30143668	3	34	theme	genomes	455:461	arg1	analysis					424:431	a comprehensive phylogenetic analysis	395:431	a comprehensive phylogenetic analysis of 74 sequenced plant genomes	395:461	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	2	35	theme	biosynthetic	310:321	arg1	enzymes					323:329	biosynthetic enzymes	310:329	biosynthetic enzymes involved in this process	310:354	However, the details regarding the pathway for starch biosynthesis and the diversity of biosynthetic enzymes involved in this process are poorly understood.					
30143668	8	36	theme	comprehensive	1465:1477	arg1	atlas					1479:1483	a comprehensive atlas	1463:1483	a comprehensive atlas of the starch biosynthetic pathway	1463:1518	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	7	37	theme	functional	1398:1407	arg1	complexes					1409:1417	functional complexes	1398:1417	functional complexes	1398:1417	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	6	38	theme	structure	1025:1033	arg1	analysis					1035:1042	A structure analysis	1023:1042	A structure analysis of these proteins	1023:1060	A structure analysis of these proteins suggested that the deletion/mutation of amino acids in some active sites resulted in not only structural variation but also sub-functionalization or neo-functionalization.					
30143668	7	39	theme	SBE-	1284:1287	arg1	genes					1306:1310	AGPase-, SS-, SBE- and DBE-encoding genes	1270:1310	AGPase-, SS-, SBE- and DBE-encoding genes	1270:1310	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	0	40	theme	expression	29:38	arg1	analysis					40:47	expression analysis	29:47	expression analysis	29:47	Evolutionary, structural and expression analysis of core genes involved in starch synthesis.					
30143668	8	41	theme	biosynthesis	1610:1621	arg1	understanding					1586:1598	understanding	1586:1598	understanding of starch biosynthesis	1586:1621	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	8	41	theme	biosynthesis	1610:1621	arg1	divergence					1655:1664	the functional evolutionary divergence	1627:1664	the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants	1627:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	8	42	theme	AGPase	1669:1674	arg1	understanding					1586:1598	understanding	1586:1598	understanding of starch biosynthesis	1586:1621	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	8	42	theme	AGPase	1669:1674	arg1	divergence					1655:1664	the functional evolutionary divergence	1627:1664	the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants	1627:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	7	43	theme	divergent	1338:1346	arg1	patterns					1359:1366	spatio-temporally divergent expression patterns	1320:1366	spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis	1320:1440	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	5	44	theme	observed	982:989	arg1	differences					991:1001	the observed differences	978:1001	the observed differences in isoform numbers	978:1020	The results showed that AGPase, SS, SBE and DBE have undergone complicated evolutionary processes in plants and that gene/genome duplications are responsible for the observed differences in isoform numbers.					
30143668	3	45	theme	sequenced	439:447	arg1	genomes					455:461	74 sequenced plant genomes	436:461	74 sequenced plant genomes	436:461	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	0	46	theme	core	52:55	arg1	genes					57:61	core genes	52:61	core genes	52:61	Evolutionary, structural and expression analysis of core genes involved in starch synthesis.					
30143668	8	47	theme	starch	1603:1608	arg1	biosynthesis					1610:1621	starch biosynthesis	1603:1621	starch biosynthesis	1603:1621	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	6	48	from	deletion/mutation	1081:1097	arg1	sites					1129:1133	some active sites	1117:1133	some active sites	1117:1133	A structure analysis of these proteins suggested that the deletion/mutation of amino acids in some active sites resulted in not only structural variation but also sub-functionalization or neo-functionalization.					
30143668	2	49	theme	enzymes	323:329	arg1	diversity					297:305	the diversity	293:305	the diversity of biosynthetic enzymes involved in this process	293:354	However, the details regarding the pathway for starch biosynthesis and the diversity of biosynthetic enzymes involved in this process are poorly understood.					
30143668	2	49	theme	enzymes	323:329	arg1	details					235:241	the details	231:241	the details regarding the pathway for starch biosynthesis	231:287	However, the details regarding the pathway for starch biosynthesis and the diversity of biosynthetic enzymes involved in this process are poorly understood.					
30143668	5	50	theme	isoform	1006:1012	arg1	numbers					1014:1020	isoform numbers	1006:1020	isoform numbers	1006:1020	The results showed that AGPase, SS, SBE and DBE have undergone complicated evolutionary processes in plants and that gene/genome duplications are responsible for the observed differences in isoform numbers.					
30143668	8	51	theme	starch	1492:1497	arg1	pathway					1512:1518	the starch biosynthetic pathway	1488:1518	the starch biosynthetic pathway	1488:1518	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	7	52	theme	SS-	1279:1281	arg1	genes					1306:1310	AGPase-, SS-, SBE- and DBE-encoding genes	1270:1310	AGPase-, SS-, SBE- and DBE-encoding genes	1270:1310	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	1	53	theme	main	107:110	arg1	carbohydrate					120:131	the main storage carbohydrate	103:131	the main storage carbohydrate in plants	103:141	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	1	53	theme	main	107:110	arg1	Starch					93:98	Starch	93:98	Starch	93:98	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	4	54	from	structures	677:686	arg1	biosynthesis					747:758	starch biosynthesis	740:758	starch biosynthesis	740:758	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	6	55	theme	structural	1156:1165	arg1	variation					1167:1175	structural variation	1156:1175	not only structural variation but also sub-functionalization or neo-functionalization	1147:1231	A structure analysis of these proteins suggested that the deletion/mutation of amino acids in some active sites resulted in not only structural variation but also sub-functionalization or neo-functionalization.					
30143668	4	56	theme	starch	740:745	arg1	biosynthesis					747:758	starch biosynthesis	740:758	starch biosynthesis	740:758	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	3	57	theme	branching	590:598	arg1	SBE					608:610	SBE	608:610	SBE	608:610	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	3	57	theme	branching	590:598	arg1	enzyme					600:605	starch branching enzyme	583:605	starch branching enzyme (SBE)	583:611	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	7	58	theme	complexes	1409:1417	arg1	composition					1383:1393	the composition	1379:1393	the composition of functional complexes in starch biosynthesis	1379:1440	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	1	59	theme	storage	112:118	arg1	carbohydrate					120:131	the main storage carbohydrate	103:131	the main storage carbohydrate in plants	103:141	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	1	59	theme	storage	112:118	arg1	Starch					93:98	Starch	93:98	Starch	93:98	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	8	60	theme	SBE	1681:1683	arg1	understanding					1586:1598	understanding	1586:1598	understanding of starch biosynthesis	1586:1621	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	8	60	theme	SBE	1681:1683	arg1	divergence					1655:1664	the functional evolutionary divergence	1627:1664	the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants	1627:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	1	61	theme	industrial	196:205	arg1	materials					211:219	industrial raw materials	196:219	industrial raw materials	196:219	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	4	62	theme	core	726:729	arg1	genes					731:735	these four core genes	715:735	these four core genes	715:735	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	3	63	theme	starch	617:622	arg1	DBE					645:647	DBE	645:647	DBE	645:647	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	3	63	theme	starch	617:622	arg1	enzyme					637:642	starch de-branching enzyme	617:642	starch de-branching enzyme (DBE)	617:648	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	1	64	from	resource	168:175	arg1	plants					136:141	plants	136:141	plants	136:141	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	4	65	theme	protein	669:675	arg1	structures					677:686	protein structures	669:686	protein structures	669:686	Additionally, the protein structures and expression patterns of these four core genes in starch biosynthesis were studied to determine their functional differences.					
30143668	8	66	theme	DBE	1690:1692	arg1	understanding					1586:1598	understanding	1586:1598	understanding of starch biosynthesis	1586:1621	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	8	66	theme	DBE	1690:1692	arg1	divergence					1655:1664	the functional evolutionary divergence	1627:1664	the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants	1627:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	1	67	theme	raw	207:209	arg1	materials					211:219	industrial raw materials	196:219	industrial raw materials	196:219	Starch is the main storage carbohydrate in plants and an important natural resource for food, feed and industrial raw materials.					
30143668	3	68	theme	de-branching	624:635	arg1	DBE					645:647	DBE	645:647	DBE	645:647	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	3	68	theme	de-branching	624:635	arg1	enzyme					637:642	starch de-branching enzyme	617:642	starch de-branching enzyme (DBE)	617:648	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	7	69	theme	DBE-encoding	1293:1304	arg1	genes					1306:1310	AGPase-, SS-, SBE- and DBE-encoding genes	1270:1310	AGPase-, SS-, SBE- and DBE-encoding genes	1270:1310	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	7	70	theme	expression	1348:1357	arg1	patterns					1359:1366	spatio-temporally divergent expression patterns	1320:1366	spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis	1320:1440	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	0	71	theme	genes	57:61	arg1	Evolutionary					0:11	Evolutionary	0:11	Evolutionary	0:11	Evolutionary, structural and expression analysis of core genes involved in starch synthesis.					
30143668	8	72	theme	evolutionary	1642:1653	arg1	divergence					1655:1664	the functional evolutionary divergence	1627:1664	the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants	1627:1702	This study provides a comprehensive atlas of the starch biosynthetic pathway, and these data should support future studies aimed at increasing understanding of starch biosynthesis and the functional evolutionary divergence of AGPase, SS, SBE, and DBE in plants.					
30143668	3	73	theme	starch	583:588	arg1	SBE					608:610	SBE	608:610	SBE	608:610	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	3	73	theme	starch	583:588	arg1	enzyme					600:605	starch branching enzyme	583:605	starch branching enzyme (SBE)	583:611	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	7	74	theme	AGPase-	1270:1276	arg1	genes					1306:1310	AGPase-, SS-, SBE- and DBE-encoding genes	1270:1310	AGPase-, SS-, SBE- and DBE-encoding genes	1270:1310	Expression profiling indicated that AGPase-, SS-, SBE- and DBE-encoding genes exhibit spatio-temporally divergent expression patterns related to the composition of functional complexes in starch biosynthesis.					
30143668	3	75	theme	genes	506:510	arg1	history					491:497	the evolutionary history	474:497	the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE)	474:648	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30143668	3	76	theme	evolutionary	478:489	arg1	history					491:497	the evolutionary history	474:497	the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE)	474:648	This study uses a comprehensive phylogenetic analysis of 74 sequenced plant genomes to revisit the evolutionary history of the genes encoding ADP-glucose pyrophosphorylase (AGPase), starch synthase (SS), starch branching enzyme (SBE) and starch de-branching enzyme (DBE).					
30736731	2	0	theme	bile	387:390	arg1	composition					397:407	host bile pool composition	382:407	host bile pool composition	382:407	This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease.					
30736731	6	1	theme	porcine	959:965	arg1	profiling					946:954	Bile acid profiling	936:954	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426	936:1038	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	4	2	theme	same	636:639	arg1	species					641:647	the same species	632:647	the same species	632:647	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	9	3	theme	nitric	1579:1584	arg1	oxide					1586:1590	nitric oxide	1579:1590	nitric oxide	1579:1590	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	8	4	theme	Gene	1282:1285	arg1	data					1298:1301	Gene expression data	1282:1301	Gene expression data	1282:1301	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	8	5	theme	interleukin-6	1356:1368	arg1	transcription					1394:1406	interleukin-6 and interleukin-10 gene transcription	1356:1406	interleukin-6 and interleukin-10 gene transcription in the murine macrophages	1356:1432	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	7	6	theme	mucosae	1185:1191	arg1	DPC					1193:1195	Lactobacillus mucosae DPC 6426	1171:1200	Lactobacillus mucosae DPC 6426	1171:1200	In addition, the purified exopolysaccharide and secretome of Lactobacillus mucosae DPC 6426 were investigated for immunomodulatory capabilities using RAW264.7 macrophages.					
30736731	5	7	theme	Lactobacillus	861:873	arg1	DPC					883:885	Lactobacillus mucosae DPC 6426	861:890	Lactobacillus mucosae DPC 6426	861:890	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	6	8	theme	Bile	936:939	arg1	profiling					946:954	Bile acid profiling	936:954	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426	936:1038	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	5	9	dep	Lactobacillus	826:838	arg1	reuteri					840:846	reuteri	840:846	reuteri	840:846	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	6	10	with	incubation	993:1002	arg1	6426					1035:1038	Lactobacillus mucosae DPC 6426	1009:1038	Lactobacillus mucosae DPC 6426	1009:1038	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	8	11	theme	interleukin-10	1374:1387	arg1	transcription					1394:1406	interleukin-6 and interleukin-10 gene transcription	1356:1406	interleukin-6 and interleukin-10 gene transcription in the murine macrophages	1356:1432	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	10	12	theme	DPC	1784:1786	arg1	6426					1788:1791	Lactobacillus mucosae DPC 6426	1762:1791	Lactobacillus mucosae DPC 6426	1762:1791	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	1	13	theme	dyslipidaemia	208:220	arg1	form					200:203	the form	196:203	the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model	196:301	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	10	14	theme	Lactobacillus	1762:1774	arg1	6426					1788:1791	Lactobacillus mucosae DPC 6426	1762:1791	Lactobacillus mucosae DPC 6426	1762:1791	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	1	15	theme	hypercholesterolemia	226:245	arg1	form					200:203	the form	196:203	the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model	196:301	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	6	16	theme	mucosae	1023:1029	arg1	6426					1035:1038	Lactobacillus mucosae DPC 6426	1009:1038	Lactobacillus mucosae DPC 6426	1009:1038	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	4	17	theme	bile	673:676	arg1	salt					678:681	a confirmed bile salt	661:681	a confirmed bile salt hydrolase-active strain	661:705	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	10	18	theme	bile	1808:1811	arg1	composition					1818:1828	bile pool composition	1808:1828	bile pool composition	1808:1828	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	7	19	theme	purified	1127:1134	arg1	exopolysaccharide					1136:1152	exopolysaccharide	1136:1152	exopolysaccharide	1136:1152	In addition, the purified exopolysaccharide and secretome of Lactobacillus mucosae DPC 6426 were investigated for immunomodulatory capabilities using RAW264.7 macrophages.					
30736731	5	20	theme	mucosae	875:881	arg1	DPC					883:885	Lactobacillus mucosae DPC 6426	861:890	Lactobacillus mucosae DPC 6426	861:890	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	5	21	theme	greatest	908:915	arg1	activity					917:924	the greatest activity	904:924	the greatest activity	904:924	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	9	22	theme	tumour	1675:1680	arg1	secretion					1700:1708	increased tumour necrosis factor-α secretion	1665:1708	increased tumour necrosis factor-α secretion at all doses	1665:1721	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	6	23	theme	bile	1098:1101	arg1	acids					1103:1107	glyco-conjugated bile acids	1081:1107	glyco-conjugated bile acids	1081:1107	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	8	24	theme	entire	1445:1450	arg1	necessary					1466:1474	necessary	1466:1474	necessary	1466:1474	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	8	24	theme	entire	1445:1450	arg1	secretome					1452:1460	the entire secretome	1441:1460	the entire secretome	1441:1460	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	5	25	theme	enzymatic	802:810	arg1	extracts					812:819	enzymatic extracts	802:819	enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426	802:890	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	8	26	from	transcription	1394:1406	arg1	macrophages					1422:1432	the murine macrophages	1411:1432	the murine macrophages	1411:1432	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	9	27	theme	factor-α	1691:1698	arg1	secretion					1700:1708	increased tumour necrosis factor-α secretion	1665:1708	increased tumour necrosis factor-α secretion at all doses	1665:1721	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	5	28	theme	hydrolase	771:779	arg1	assays					781:786	Quantitative bile salt hydrolase assays	748:786	Quantitative bile salt hydrolase assays	748:786	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	8	29	theme	CD206	1488:1492	arg1	transcription					1494:1506	CD206 transcription	1488:1506	CD206 transcription	1488:1506	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	3	30	theme	Lactobacillus	472:484	arg1	6426					498:501	Lactobacillus mucosae DPC 6426	472:501	Lactobacillus mucosae DPC 6426	472:501	Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity.					
30736731	1	31	theme	BACKGROUND	81:90	arg1	6426					118:121	BACKGROUND Lactobacillus mucosae DPC 6426	81:121	BACKGROUND Lactobacillus mucosae DPC 6426	81:121	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	9	32	with	concurrent	1649:1658	arg1	secretion					1700:1708	increased tumour necrosis factor-α secretion	1665:1708	increased tumour necrosis factor-α secretion at all doses	1665:1721	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	0	33	theme	Lactobacillus	0:12	arg1	DPC					22:24	Lactobacillus mucosae DPC	0:24	Lactobacillus mucosae DPC	0:24	Lactobacillus mucosae DPC 6426 as a bile-modifying and immunomodulatory microbe.					
30736731	1	34	theme	mucosae	106:112	arg1	6426					118:121	BACKGROUND Lactobacillus mucosae DPC 6426	81:121	BACKGROUND Lactobacillus mucosae DPC 6426	81:121	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	2	35	theme	cardiovascular	448:461	arg1	disease					463:469	cardiovascular disease	448:469	cardiovascular disease	448:469	This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease.					
30736731	9	36	theme	necrosis	1682:1689	arg1	secretion					1700:1708	increased tumour necrosis factor-α secretion	1665:1708	increased tumour necrosis factor-α secretion at all doses	1665:1721	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	4	37	theme	other	607:611	arg1	strains					621:627	several other enteric strains	599:627	several other enteric strains of the same species	599:647	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	3	38	theme	DPC	494:496	arg1	6426					498:501	Lactobacillus mucosae DPC 6426	472:501	Lactobacillus mucosae DPC 6426	472:501	Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity.					
30736731	4	39	theme	hydrolase-active	683:698	arg1	strain					700:705	a confirmed bile salt hydrolase-active strain	661:705	a confirmed bile salt hydrolase-active strain	661:705	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	9	40	from	increase	1567:1574	arg1	oxide					1586:1590	nitric oxide	1579:1590	nitric oxide	1579:1590	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	9	40	from	increase	1567:1574	arg1	production					1611:1620	interleukin-10 production	1596:1620	interleukin-10 production	1596:1620	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	0	41	theme	immunomodulatory	55:70	arg1	microbe					72:78	a bile-modifying and immunomodulatory microbe	34:78	a bile-modifying and immunomodulatory microbe	34:78	Lactobacillus mucosae DPC 6426 as a bile-modifying and immunomodulatory microbe.					
30736731	3	42	theme	salt	525:528	arg1	activity					540:547	bile salt hydrolase activity	520:547	bile salt hydrolase activity	520:547	Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity.					
30736731	7	43	theme	immunomodulatory	1224:1239	arg1	capabilities					1241:1252	immunomodulatory capabilities	1224:1252	immunomodulatory capabilities using RAW264.7 macrophages	1224:1279	In addition, the purified exopolysaccharide and secretome of Lactobacillus mucosae DPC 6426 were investigated for immunomodulatory capabilities using RAW264.7 macrophages.					
30736731	10	44	theme	cardio-protective	1929:1945	arg1	phenotype					1947:1955	the previously identified cardio-protective phenotype	1903:1955	the previously identified cardio-protective phenotype	1903:1955	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	4	45	theme	species	641:647	arg1	strains					621:627	several other enteric strains	599:627	several other enteric strains of the same species	599:647	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	4	45	theme	species	641:647	arg1	2587					734:737	Lactobacillus reuteri APC 2587	708:737	Lactobacillus reuteri APC 2587	708:737	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	4	45	theme	species	641:647	arg1	strain					700:705	a confirmed bile salt hydrolase-active strain	661:705	a confirmed bile salt hydrolase-active strain	661:705	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	10	46	theme	identified	1918:1927	arg1	phenotype					1947:1955	the previously identified cardio-protective phenotype	1903:1955	the previously identified cardio-protective phenotype	1903:1955	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	1	47	theme	deficient	281:289	arg1	model					297:301	an apolipoprotein-E deficient mouse model	261:301	an apolipoprotein-E deficient mouse model	261:301	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	6	48	theme	bile	978:981	arg1	profiling					946:954	Bile acid profiling	936:954	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426	936:1038	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	2	49	theme	pool	392:395	arg1	composition					397:407	host bile pool composition	382:407	host bile pool composition	382:407	This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease.					
30736731	9	50	theme	dose-dependent	1552:1565	arg1	increase					1567:1574	a dose-dependent increase	1550:1574	a dose-dependent increase in nitric oxide and interleukin-10 production	1550:1620	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	7	51	theme	DPC	1193:1195	arg1	secretome					1158:1166	secretome	1158:1166	secretome	1158:1166	In addition, the purified exopolysaccharide and secretome of Lactobacillus mucosae DPC 6426 were investigated for immunomodulatory capabilities using RAW264.7 macrophages.					
30736731	7	51	theme	DPC	1193:1195	arg1	exopolysaccharide					1136:1152	exopolysaccharide	1136:1152	exopolysaccharide	1136:1152	In addition, the purified exopolysaccharide and secretome of Lactobacillus mucosae DPC 6426 were investigated for immunomodulatory capabilities using RAW264.7 macrophages.					
30736731	8	52	from	increases	1343:1351	arg1	transcription					1394:1406	interleukin-6 and interleukin-10 gene transcription	1356:1406	interleukin-6 and interleukin-10 gene transcription in the murine macrophages	1356:1432	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	2	53	theme	host	382:385	arg1	composition					397:407	host bile pool composition	382:407	host bile pool composition	382:407	This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease.					
30736731	7	54	theme	Lactobacillus	1171:1183	arg1	DPC					1193:1195	Lactobacillus mucosae DPC 6426	1171:1200	Lactobacillus mucosae DPC 6426	1171:1200	In addition, the purified exopolysaccharide and secretome of Lactobacillus mucosae DPC 6426 were investigated for immunomodulatory capabilities using RAW264.7 macrophages.					
30736731	0	55	theme	bile-modifying	36:49	arg1	microbe					72:78	a bile-modifying and immunomodulatory microbe	34:78	a bile-modifying and immunomodulatory microbe	34:78	Lactobacillus mucosae DPC 6426 as a bile-modifying and immunomodulatory microbe.					
30736731	6	56	theme	acid	941:944	arg1	profiling					946:954	Bile acid profiling	936:954	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426	936:1038	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	4	57	theme	salt	678:681	arg1	strain					700:705	a confirmed bile salt hydrolase-active strain	661:705	a confirmed bile salt hydrolase-active strain	661:705	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	9	58	theme	interleukin-10	1596:1609	arg1	production					1611:1620	interleukin-10 production	1596:1620	interleukin-10 production	1596:1620	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	8	59	theme	expression	1287:1296	arg1	data					1298:1301	Gene expression data	1282:1301	Gene expression data	1282:1301	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	10	60	theme	mucosae	1776:1782	arg1	6426					1788:1791	Lactobacillus mucosae DPC 6426	1762:1791	Lactobacillus mucosae DPC 6426	1762:1791	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	6	61	theme	DPC	1031:1033	arg1	6426					1035:1038	Lactobacillus mucosae DPC 6426	1009:1038	Lactobacillus mucosae DPC 6426	1009:1038	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	8	62	theme	gene	1389:1392	arg1	transcription					1394:1406	interleukin-6 and interleukin-10 gene transcription	1356:1406	interleukin-6 and interleukin-10 gene transcription in the murine macrophages	1356:1432	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	10	63	theme	pool	1813:1816	arg1	composition					1818:1828	bile pool composition	1808:1828	bile pool composition	1808:1828	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	5	64	theme	Quantitative	748:759	arg1	assays					781:786	Quantitative bile salt hydrolase assays	748:786	Quantitative bile salt hydrolase assays	748:786	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	6	65	theme	Lactobacillus	1009:1021	arg1	6426					1035:1038	Lactobacillus mucosae DPC 6426	1009:1038	Lactobacillus mucosae DPC 6426	1009:1038	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	4	66	theme	confirmed	663:671	arg1	salt					678:681	a confirmed bile salt	661:681	a confirmed bile salt hydrolase-active strain	661:705	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	8	67	theme	murine	1415:1420	arg1	macrophages					1422:1432	the murine macrophages	1411:1432	the murine macrophages	1411:1432	Gene expression data revealed that both fractions stimulated increases in interleukin-6 and interleukin-10 gene transcription in the murine macrophages, while the entire secretome was necessary to increase CD206 transcription.					
30736731	10	68	dep	CONCLUSIONS	1724:1734	arg1	indicates					1747:1755	indicates	1747:1755	indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype	1747:1955	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	1	69	from	hypercholesterolemia	226:245	arg1	model					297:301	an apolipoprotein-E deficient mouse model	261:301	an apolipoprotein-E deficient mouse model	261:301	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	9	70	theme	increased	1665:1673	arg1	secretion					1700:1708	increased tumour necrosis factor-α secretion	1665:1708	increased tumour necrosis factor-α secretion at all doses	1665:1721	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	6	71	theme	acids	1103:1107	arg1	hydrolysis					1067:1076	hydrolysis	1067:1076	hydrolysis of glyco-conjugated bile acids	1067:1107	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	1	72	from	dyslipidaemia	208:220	arg1	model					297:301	an apolipoprotein-E deficient mouse model	261:301	an apolipoprotein-E deficient mouse model	261:301	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	1	73	theme	cardio-protective	163:179	arg1	properties					181:190	potentially cardio-protective properties	151:190	potentially cardio-protective properties	151:190	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	6	74	theme	glyco-conjugated	1081:1096	arg1	acids					1103:1107	glyco-conjugated bile acids	1081:1107	glyco-conjugated bile acids	1081:1107	Bile acid profiling of porcine and murine bile following incubation with Lactobacillus mucosae DPC 6426 confirmed a preference for hydrolysis of glyco-conjugated bile acids.					
30736731	4	75	theme	Lactobacillus	708:720	arg1	2587					734:737	Lactobacillus reuteri APC 2587	708:737	Lactobacillus reuteri APC 2587	708:737	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	5	76	from	2587	852:855	arg1	extracts					812:819	enzymatic extracts	802:819	enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426	802:890	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	1	77	from	form	200:203	arg1	model					297:301	an apolipoprotein-E deficient mouse model	261:301	an apolipoprotein-E deficient mouse model	261:301	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	5	78	dep	RESULTS	740:746	arg1	revealed					788:795	revealed	788:795	revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro	788:933	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	4	79	theme	APC	730:732	arg1	2587					734:737	Lactobacillus reuteri APC 2587	708:737	Lactobacillus reuteri APC 2587	708:737	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	5	80	theme	salt	766:769	arg1	assays					781:786	Quantitative bile salt hydrolase assays	748:786	Quantitative bile salt hydrolase assays	748:786	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	9	81	from	doses	1717:1721	arg1	secretion					1700:1708	increased tumour necrosis factor-α secretion	1665:1708	increased tumour necrosis factor-α secretion at all doses	1665:1721	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	5	82	from	DPC	883:885	arg1	extracts					812:819	enzymatic extracts	802:819	enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426	802:890	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	9	83	theme	RAW264.7	1627:1634	arg1	macrophages					1636:1646	RAW264.7 macrophages	1627:1646	RAW264.7 macrophages	1627:1646	Moreover, the exopolysaccharide elicited a dose-dependent increase in nitric oxide and interleukin-10 production from RAW264.7 macrophages, concurrent with increased tumour necrosis factor-α secretion at all doses.					
30736731	3	84	theme	mucosae	486:492	arg1	6426					498:501	Lactobacillus mucosae DPC 6426	472:501	Lactobacillus mucosae DPC 6426	472:501	Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity.					
30736731	0	85	theme	mucosae	14:20	arg1	DPC					22:24	Lactobacillus mucosae DPC	0:24	Lactobacillus mucosae DPC	0:24	Lactobacillus mucosae DPC 6426 as a bile-modifying and immunomodulatory microbe.					
30736731	1	86	theme	Lactobacillus	92:104	arg1	6426					118:121	BACKGROUND Lactobacillus mucosae DPC 6426	81:121	BACKGROUND Lactobacillus mucosae DPC 6426	81:121	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	1	87	from	model	297:301	arg1	form					200:203	the form	196:203	the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model	196:301	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	4	88	theme	several	599:605	arg1	strains					621:627	several other enteric strains	599:627	several other enteric strains of the same species	599:647	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	2	89	theme	immune	413:418	arg1	response					420:427	immune response	413:427	immune response	413:427	This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease.					
30736731	7	90	theme	RAW264.7	1260:1267	arg1	macrophages					1269:1279	RAW264.7 macrophages	1260:1279	RAW264.7 macrophages	1260:1279	In addition, the purified exopolysaccharide and secretome of Lactobacillus mucosae DPC 6426 were investigated for immunomodulatory capabilities using RAW264.7 macrophages.					
30736731	1	91	theme	DPC	114:116	arg1	6426					118:121	BACKGROUND Lactobacillus mucosae DPC 6426	81:121	BACKGROUND Lactobacillus mucosae DPC 6426	81:121	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	2	92	theme	disease	463:469	arg1	context					437:443	the context	433:443	the context of cardiovascular disease	433:469	This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease.					
30736731	4	93	theme	enteric	613:619	arg1	strains					621:627	several other enteric strains	599:627	several other enteric strains of the same species	599:647	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	5	94	theme	Lactobacillus	826:838	arg1	2587					852:855	Lactobacillus reuteri APC 2587	826:855	Lactobacillus reuteri APC 2587	826:855	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	10	95	theme	system	1841:1846	arg1	tone					1848:1851	immune system tone	1834:1851	immune system tone	1834:1851	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	1	96	dep	dyslipidaemia	208:220	arg1	correction					247:256	correction	247:256	correction	247:256	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	5	97	theme	APC	848:850	arg1	2587					852:855	Lactobacillus reuteri APC 2587	826:855	Lactobacillus reuteri APC 2587	826:855	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	5	98	theme	bile	761:764	arg1	assays					781:786	Quantitative bile salt hydrolase assays	748:786	Quantitative bile salt hydrolase assays	748:786	RESULTS Quantitative bile salt hydrolase assays revealed that enzymatic extracts from Lactobacillus reuteri APC 2587 and Lactobacillus mucosae DPC 6426 demonstrate the greatest activity in vitro.					
30736731	3	99	theme	hydrolase	530:538	arg1	activity					540:547	bile salt hydrolase activity	520:547	bile salt hydrolase activity	520:547	Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity.					
30736731	4	100	dep	Lactobacillus	708:720	arg1	reuteri					722:728	reuteri	722:728	reuteri	722:728	The microbe was compared against several other enteric strains of the same species, as well as a confirmed bile salt hydrolase-active strain, Lactobacillus reuteri APC 2587.					
30736731	3	101	theme	bile	520:523	arg1	activity					540:547	bile salt hydrolase activity	520:547	bile salt hydrolase activity	520:547	Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity.					
30736731	1	102	theme	apolipoprotein-E	264:279	arg1	model					297:301	an apolipoprotein-E deficient mouse model	261:301	an apolipoprotein-E deficient mouse model	261:301	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30736731	10	103	theme	immune	1834:1839	arg1	system					1841:1846	immune system	1834:1846	immune system tone	1834:1851	CONCLUSIONS This study indicates that Lactobacillus mucosae DPC 6426 modulates both bile pool composition and immune system tone in a manner which may contribute significantly to the previously identified cardio-protective phenotype.					
30736731	1	104	theme	mouse	291:295	arg1	model					297:301	an apolipoprotein-E deficient mouse model	261:301	an apolipoprotein-E deficient mouse model	261:301	BACKGROUND Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model.					
30852013	4	0	with	design	762:767	arg1	periods					779:785	18-d periods	774:785	18-d periods (15 d of adaptation and 3 d of sampling)	774:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	0	with	design	762:767	arg1	d					791:791	15 d	788:791	15 d of adaptation	788:805	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	0	with	design	762:767	arg1	d					813:813	3 d	811:813	3 d of sampling	811:825	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	3	1	theme	starch	566:571	arg1	distribution					573:584	starch distribution	566:584	starch distribution	566:584	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	13	2	theme	composite	2173:2181	arg1	sample					2183:2188	a daily total composite sample	2159:2188	a daily total composite sample	2159:2188	Average retained fecal particle DM and NDF content varied depending on the time after feeding, resulting in the large differences between grab fecal samples and a daily total composite sample.					
30852013	1	3	theme	diets	211:215	arg1	utilization					196:206	the nutrient utilization	183:206	the nutrient utilization of diets by dairy cows	183:229	Wet sieving of manure can be used as a means of evaluating the nutrient utilization of diets by dairy cows.					
30852013	7	4	theme	grab	1106:1109	arg1	samples					1111:1117	Fecal grab samples	1100:1117	Fecal grab samples	1100:1117	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	13	5	theme	fecal	2015:2019	arg1	DM					2030:2031	fecal particle DM	2015:2031	fecal particle DM	2015:2031	Average retained fecal particle DM and NDF content varied depending on the time after feeding, resulting in the large differences between grab fecal samples and a daily total composite sample.					
30852013	3	6	theme	manipulations	458:470	arg1	effects					442:448	effects	442:448	effects of diet manipulations	442:470	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	6	theme	manipulations	458:470	arg1	techniques					485:494	sampling techniques	476:494	sampling techniques	476:494	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	13	7	theme	fecal	2141:2145	arg1	samples					2147:2153	grab fecal samples	2136:2153	grab fecal samples	2136:2153	Average retained fecal particle DM and NDF content varied depending on the time after feeding, resulting in the large differences between grab fecal samples and a daily total composite sample.					
30852013	5	8	theme	NDF	942:944	arg1	%					949:949	low NDF <40%	938:949	low NDF <40%	938:949	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	8	theme	NDF	942:944	arg1	%					935:935	high NDF >45%	923:935	high NDF >45%	923:935	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	11	9	theme	NDF	1673:1675	arg1	content					1677:1683	The NDF content	1669:1683	The NDF content in retained material	1669:1704	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	10	10	dep	20.37	1647:1651	arg1	to					1644:1645	to	1644:1645	to	1644:1645	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	9	11	theme	feeding	1471:1477	arg1	regimen					1479:1485	feeding regimen	1471:1485	feeding regimen	1471:1485	Sampling day and feeding regimen did not affect distributions of nutrients.					
30852013	14	12	theme	particle	2227:2234	arg1	DM					2236:2237	retained particle DM	2218:2237	retained particle DM	2218:2237	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	14	13	theme	time	2271:2274	arg1	points					2276:2281	all sampling time points	2258:2281	all sampling time points	2258:2281	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	13	14	theme	large	2110:2114	arg1	differences					2116:2126	the large differences	2106:2126	the large differences between grab fecal samples and a daily total composite sample	2106:2188	Average retained fecal particle DM and NDF content varied depending on the time after feeding, resulting in the large differences between grab fecal samples and a daily total composite sample.					
30852013	7	15	theme	total	1254:1258	arg1	collection					1266:1275	total fecal collection	1254:1275	total fecal collection	1254:1275	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	4	16	theme	Latin	736:740	arg1	square					742:747	3 incomplete Latin square	723:747	a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling)	706:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	11	17	theme	fiber	1809:1813	arg1	content					1815:1821	fiber content	1809:1821	fiber content	1809:1821	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	14	18	from	sample	2331:2336	arg1	ones					2299:2302	ones	2299:2302	ones from total daily composite sample	2299:2336	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	2	19	theme	available	247:255	arg1	system					257:262	A commercially available system	232:262	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA)	232:338	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	4	20	theme	experimental	749:760	arg1	design					762:767	a split-plot 4 × 3 incomplete Latin square experimental design	706:767	a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling)	706:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	12	21	theme	diets	1991:1995	arg1	majority					1979:1986	the majority	1975:1986	the majority of diets	1975:1995	Starch content in retained material was below 0.5% in the majority of diets.					
30852013	14	22	theme	weight	2247:2252	arg1	values					2208:2213	average values	2200:2213	average values of retained particle DM and NDF weight for all sampling time points	2200:2281	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	10	23	theme	DM	1568:1569	arg1	Distributions					1530:1542	Distributions	1530:1542	Distributions of total fecal particle DM	1530:1569	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	14	24	theme	composite	2321:2329	arg1	sample					2331:2336	total daily composite sample	2309:2336	total daily composite sample	2309:2336	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	8	25	theme	NDA	1357:1359	arg1	kit					1361:1363	a NDA kit	1355:1363	a NDA kit	1355:1363	After wet sieving of each sample using a NDA kit, the retained material on sieves was dried and analyzed for DM, NDF, and starch content.					
30852013	11	26	theme	DM	1910:1911	arg1	weight					1913:1918	the retained particle DM weight	1888:1918	the retained particle DM weight	1888:1918	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	2	27	dep	system	257:262	arg1	WI					331:332	WI	331:332	WI	331:332	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	2	27	dep	system	257:262	arg1	Analyzer					299:306	Nasco Digestion Analyzer	283:306	Nasco Digestion Analyzer	283:306	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	10	28	theme	fecal	1553:1557	arg1	DM					1568:1569	total fecal particle DM	1547:1569	total fecal particle DM	1547:1569	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	14	29	theme	total	2309:2313	arg1	sample					2331:2336	total daily composite sample	2309:2336	total daily composite sample	2309:2336	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	5	30	theme	Treatment	829:837	arg1	rations					839:845	Treatment rations	829:845	Treatment rations	829:845	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	3	31	theme	particle	505:512	arg1	DM					526:527	DM	526:527	DM	526:527	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	31	theme	particle	505:512	arg1	matter					518:523	fecal particle dry matter	499:523	fecal particle dry matter (DM)	499:528	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	10	32	theme	DM	1664:1665	arg1	DM					1664:1665	sample DM	1657:1665	sample DM	1657:1665	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	10	32	theme	DM	1664:1665	arg1	%					1652:1652	14.66 to 20.37%	1638:1652	14.66 to 20.37% of sample DM	1638:1665	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	1	33	used	used	153:156	arg2	means					163:167	a means	161:167	a means of evaluating the nutrient utilization of diets by dairy cows	161:229	Wet sieving of manure can be used as a means of evaluating the nutrient utilization of diets by dairy cows.					
30852013	1	33	used	used	153:156	arg2	sieving					128:134	Wet sieving	124:134	Wet sieving of manure	124:144	Wet sieving of manure can be used as a means of evaluating the nutrient utilization of diets by dairy cows.					
30852013	11	34	theme	retained	1892:1899	arg1	weight					1913:1918	the retained particle DM weight	1888:1918	the retained particle DM weight	1888:1918	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	4	35	theme	Holstein	607:614	arg1	heifers					616:622	Eight Holstein heifers	601:622	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg)	601:677	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	5	36	theme	ad	984:985	arg1	libitum					987:993	ad libitum and restricted intakes	984:1016	libitum	987:993	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	10	37	theme	high	1588:1591	arg1	quality					1600:1606	high forage quality	1588:1606	high forage quality	1588:1606	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	8	38	theme	retained	1370:1377	arg1	material					1379:1386	the retained material	1366:1386	the retained material on sieves	1366:1396	After wet sieving of each sample using a NDA kit, the retained material on sieves was dried and analyzed for DM, NDF, and starch content.					
30852013	4	39	dep	heifers	616:622	arg1	SD					633:634	means ± SD	625:634	means ± SD	625:634	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	7	40	theme	remaining	1207:1215	arg1	feces					1217:1221	the remaining feces	1203:1221	the remaining feces	1203:1221	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	7	41	dep	h	1151:1151	arg1	feeding					1159:1165	feeding	1159:1165	feeding all 3 d of the sampling period	1159:1196	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	5	42	theme	low	890:892	arg1	%					887:887	high ADF <35%	875:887	high ADF <35%	875:887	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	42	theme	low	890:892	arg1	%					901:901	low ADF >35%	890:901	low ADF >35%	890:901	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	4	43	dep	×	721:721	arg1	square					742:747	3 incomplete Latin square	723:747	a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling)	706:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	1	44	theme	Wet	124:126	arg1	means					163:167	a means	161:167	a means of evaluating the nutrient utilization of diets by dairy cows	161:229	Wet sieving of manure can be used as a means of evaluating the nutrient utilization of diets by dairy cows.					
30852013	1	44	theme	Wet	124:126	arg1	sieving					128:134	Wet sieving	124:134	Wet sieving of manure	124:144	Wet sieving of manure can be used as a means of evaluating the nutrient utilization of diets by dairy cows.					
30852013	10	45	theme	high	1612:1615	arg1	content					1623:1629	high fiber content	1612:1629	high fiber content	1612:1629	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	0	46	theme	ad	64:65	arg1	libitum					67:73	ad libitum	64:73	ad libitum	64:73	Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality.					
30852013	13	47	theme	grab	2136:2139	arg1	samples					2147:2153	grab fecal samples	2136:2153	grab fecal samples	2136:2153	Average retained fecal particle DM and NDF content varied depending on the time after feeding, resulting in the large differences between grab fecal samples and a daily total composite sample.					
30852013	4	48	dep	SD	633:634	arg1	mo					652:653	age 18.4 ± 0.6 mo	637:653	age 18.4 ± 0.6 mo	637:653	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	48	dep	SD	633:634	arg1	BW					659:660	BW 457.2 ± 27.3 kg	659:676	BW 457.2 ± 27.3 kg	659:676	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	7	49	theme	period	1191:1196	arg1	d					1173:1173	all 3 d	1167:1173	all 3 d of the sampling period	1167:1196	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	5	50	theme	ADF	880:882	arg1	%					887:887	high ADF <35%	875:887	high ADF <35%	875:887	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	50	theme	ADF	880:882	arg1	%					901:901	low ADF >35%	890:901	low ADF >35%	890:901	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	6	51	dep	1,000	1050:1054	arg1	to					1047:1048	to	1047:1048	to	1047:1048	Diets were fed to allow 900 to 1,000 g/d of body weight gain and fed once daily.					
30852013	12	52	from	%	1970:1970	arg1	majority					1979:1986	the majority	1975:1986	the majority of diets	1975:1995	Starch content in retained material was below 0.5% in the majority of diets.					
30852013	7	53	theme	composite	1298:1306	arg1	sample					1308:1313	a daily composite sample	1290:1313	a daily composite sample	1290:1313	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	5	54	dep	content	914:920	arg1	%					949:949	low NDF <40%	938:949	low NDF <40%	938:949	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	54	dep	content	914:920	arg1	%					935:935	high NDF >45%	923:935	high NDF >45%	923:935	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	1	55	theme	dairy	220:224	arg1	cows					226:229	dairy cows	220:229	dairy cows	220:229	Wet sieving of manure can be used as a means of evaluating the nutrient utilization of diets by dairy cows.					
30852013	13	56	theme	particle	2021:2028	arg1	DM					2030:2031	fecal particle DM	2015:2031	fecal particle DM	2015:2031	Average retained fecal particle DM and NDF content varied depending on the time after feeding, resulting in the large differences between grab fecal samples and a daily total composite sample.					
30852013	4	57	theme	sampling	818:825	arg1	periods					779:785	18-d periods	774:785	18-d periods (15 d of adaptation and 3 d of sampling)	774:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	57	theme	sampling	818:825	arg1	d					813:813	3 d	811:813	3 d of sampling	811:825	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	57	theme	sampling	818:825	arg1	d					791:791	15 d	788:791	15 d of adaptation	788:805	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	12	58	theme	Starch	1921:1926	arg1	content					1928:1934	Starch content	1921:1934	Starch content in retained material	1921:1955	Starch content in retained material was below 0.5% in the majority of diets.					
30852013	0	59	theme	Fecal	0:4	arg1	matter					19:24	Fecal particle dry matter	0:24	Fecal particle dry matter	0:24	Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality.					
30852013	11	60	theme	same	1872:1875	arg1	pattern					1877:1883	the same pattern	1868:1883	the same pattern as the retained particle DM weight	1868:1918	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	4	61	theme	±	631:631	arg1	SD					633:634	means ± SD	625:634	means ± SD	625:634	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	0	62	theme	dry	15:17	arg1	matter					19:24	Fecal particle dry matter	0:24	Fecal particle dry matter	0:24	Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality.					
30852013	5	63	theme	NDF	928:930	arg1	%					949:949	low NDF <40%	938:949	low NDF <40%	938:949	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	63	theme	NDF	928:930	arg1	%					935:935	high NDF >45%	923:935	high NDF >45%	923:935	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	0	64	dep	heifers	52:58	arg1	restricted					79:88	restricted	79:88	restricted with low and high	79:106	Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality.					
30852013	0	64	dep	heifers	52:58	arg1	fed					60:62	fed	60:62	fed ad libitum	60:73	Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality.					
30852013	7	65	theme	fecal	1260:1264	arg1	collection					1266:1275	total fecal collection	1254:1275	total fecal collection	1254:1275	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	11	66	theme	sieve	1732:1736	arg1	size					1738:1741	sieve size	1732:1741	sieve size (from 89.81 to 86.86%)	1732:1764	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	0	67	theme	heifers	52:58	arg1	matter					19:24	Fecal particle dry matter	0:24	Fecal particle dry matter	0:24	Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality.					
30852013	0	67	theme	heifers	52:58	arg1	distribution					36:47	fiber distribution	30:47	fiber distribution	30:47	Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality.					
30852013	11	68	from	%	1763:1763	arg1	size					1738:1741	sieve size	1732:1741	sieve size (from 89.81 to 86.86%)	1732:1764	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	2	69	theme	on-farm	378:384	arg1	assessment					386:395	on-farm assessment	378:395	on-farm assessment	378:395	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	11	70	dep	86.86	1758:1762	arg1	to					1755:1756	to	1755:1756	to	1755:1756	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	6	71	theme	weight	1068:1073	arg1	gain					1075:1078	body weight gain	1063:1078	body weight gain	1063:1078	Diets were fed to allow 900 to 1,000 g/d of body weight gain and fed once daily.					
30852013	2	72	theme	Digestion	289:297	arg1	WI					331:332	WI	331:332	WI	331:332	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	2	72	theme	Digestion	289:297	arg1	Analyzer					299:306	Nasco Digestion Analyzer	283:306	Nasco Digestion Analyzer	283:306	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	13	73	theme	NDF	2037:2039	arg1	content					2041:2047	NDF content	2037:2047	NDF content	2037:2047	Average retained fecal particle DM and NDF content varied depending on the time after feeding, resulting in the large differences between grab fecal samples and a daily total composite sample.					
30852013	5	74	theme	fiber	908:912	arg1	content					914:920	fiber content	908:920	fiber content (high NDF >45%, low NDF <40%)	908:950	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	11	75	dep	quality	1797:1803	arg1	forage					1790:1795	forage	1790:1795	forage	1790:1795	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	1	76	theme	nutrient	187:194	arg1	utilization					196:206	the nutrient utilization	183:206	the nutrient utilization of diets by dairy cows	183:229	Wet sieving of manure can be used as a means of evaluating the nutrient utilization of diets by dairy cows.					
30852013	5	77	dep	quality	866:872	arg1	%					887:887	high ADF <35%	875:887	high ADF <35%	875:887	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	77	dep	quality	866:872	arg1	forage					859:864	forage	859:864	forage	859:864	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	77	dep	quality	866:872	arg1	%					901:901	low ADF >35%	890:901	low ADF >35%	890:901	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	3	78	theme	NDA	589:591	arg1	sieves					593:598	NDA sieves	589:598	NDA sieves	589:598	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	79	theme	study	420:424	arg1	objective					402:410	The objective	398:410	The objective of this study	398:424	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	13	80	theme	total	2167:2171	arg1	sample					2183:2188	a daily total composite sample	2159:2188	a daily total composite sample	2159:2188	Average retained fecal particle DM and NDF content varied depending on the time after feeding, resulting in the large differences between grab fecal samples and a daily total composite sample.					
30852013	3	81	theme	detergent	539:547	arg1	fiber					549:553	neutral detergent fiber	531:553	neutral detergent fiber (NDF)	531:559	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	81	theme	detergent	539:547	arg1	NDF					556:558	NDF	556:558	NDF	556:558	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	9	82	theme	Sampling	1454:1461	arg1	day					1463:1465	Sampling day	1454:1465	Sampling day	1454:1465	Sampling day and feeding regimen did not affect distributions of nutrients.					
30852013	8	83	theme	starch	1438:1443	arg1	content					1445:1451	starch content	1438:1451	starch content	1438:1451	After wet sieving of each sample using a NDA kit, the retained material on sieves was dried and analyzed for DM, NDF, and starch content.					
30852013	3	84	theme	diet	453:456	arg1	manipulations					458:470	diet manipulations	453:470	diet manipulations	453:470	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	5	85	theme	low	938:940	arg1	%					949:949	low NDF <40%	938:949	low NDF <40%	938:949	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	85	theme	low	938:940	arg1	%					935:935	high NDF >45%	923:935	high NDF >45%	923:935	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	1	86	theme	manure	139:144	arg1	means					163:167	a means	161:167	a means of evaluating the nutrient utilization of diets by dairy cows	161:229	Wet sieving of manure can be used as a means of evaluating the nutrient utilization of diets by dairy cows.					
30852013	1	86	theme	manure	139:144	arg1	sieving					128:134	Wet sieving	124:134	Wet sieving of manure	124:144	Wet sieving of manure can be used as a means of evaluating the nutrient utilization of diets by dairy cows.					
30852013	7	87	theme	Fecal	1100:1104	arg1	samples					1111:1117	Fecal grab samples	1100:1117	Fecal grab samples	1100:1117	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	10	88	theme	sample	1657:1662	arg1	DM					1664:1665	sample DM	1657:1665	sample DM	1657:1665	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	8	89	theme	wet	1322:1324	arg1	sieving					1326:1332	wet sieving	1322:1332	wet sieving of each sample using a NDA kit	1322:1363	After wet sieving of each sample using a NDA kit, the retained material on sieves was dried and analyzed for DM, NDF, and starch content.					
30852013	14	90	theme	sampling	2262:2269	arg1	points					2276:2281	all sampling time points	2258:2281	all sampling time points	2258:2281	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	12	91	theme	retained	1939:1946	arg1	material					1948:1955	retained material	1939:1955	retained material	1939:1955	Starch content in retained material was below 0.5% in the majority of diets.					
30852013	4	92	dep	periods	779:785	arg1	periods					779:785	18-d periods	774:785	18-d periods (15 d of adaptation and 3 d of sampling)	774:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	92	dep	periods	779:785	arg1	d					813:813	3 d	811:813	3 d of sampling	811:825	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	92	dep	periods	779:785	arg1	d					791:791	15 d	788:791	15 d of adaptation	788:805	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	93	theme	incomplete	725:734	arg1	square					742:747	3 incomplete Latin square	723:747	a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling)	706:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	10	94	dep	quality	1600:1606	arg1	diets					1631:1635	diets	1631:1635	diets	1631:1635	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	10	94	dep	quality	1600:1606	arg1	forage					1593:1598	forage	1593:1598	forage	1593:1598	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	8	95	from	material	1379:1386	arg1	sieves					1391:1396	sieves	1391:1396	sieves	1391:1396	After wet sieving of each sample using a NDA kit, the retained material on sieves was dried and analyzed for DM, NDF, and starch content.					
30852013	11	96	from	content	1677:1683	arg1	material					1697:1704	retained material	1688:1704	retained material	1688:1704	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	3	97	theme	dry	514:516	arg1	DM					526:527	DM	526:527	DM	526:527	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	97	theme	dry	514:516	arg1	matter					518:523	fecal particle dry matter	499:523	fecal particle dry matter (DM)	499:528	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	8	98	theme	sample	1342:1347	arg1	sieving					1326:1332	wet sieving	1322:1332	wet sieving of each sample using a NDA kit	1322:1363	After wet sieving of each sample using a NDA kit, the retained material on sieves was dried and analyzed for DM, NDF, and starch content.					
30852013	14	99	theme	NDF	2243:2245	arg1	weight					2247:2252	NDF weight	2243:2252	NDF weight	2243:2252	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	9	100	theme	nutrients	1519:1527	arg1	distributions					1502:1514	distributions	1502:1514	distributions of nutrients	1502:1527	Sampling day and feeding regimen did not affect distributions of nutrients.					
30852013	10	101	theme	particle	1559:1566	arg1	DM					1568:1569	total fecal particle DM	1547:1569	total fecal particle DM	1547:1569	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	14	102	theme	daily	2315:2319	arg1	sample					2331:2336	total daily composite sample	2309:2336	total daily composite sample	2309:2336	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	5	103	theme	high	875:878	arg1	%					887:887	high ADF <35%	875:887	high ADF <35%	875:887	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	103	theme	high	875:878	arg1	%					901:901	low ADF >35%	890:901	low ADF >35%	890:901	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	3	104	theme	fecal	499:503	arg1	DM					526:527	DM	526:527	DM	526:527	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	104	theme	fecal	499:503	arg1	matter					518:523	fecal particle dry matter	499:523	fecal particle dry matter (DM)	499:528	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	4	105	theme	split-plot	708:717	arg1	design					762:767	a split-plot 4 × 3 incomplete Latin square experimental design	706:767	a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling)	706:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	10	106	dep	greater	1576:1582	arg1	DM					1664:1665	sample DM	1657:1665	sample DM	1657:1665	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	10	106	dep	greater	1576:1582	arg1	%					1652:1652	14.66 to 20.37%	1638:1652	14.66 to 20.37% of sample DM	1638:1665	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	14	107	theme	DM	2236:2237	arg1	values					2208:2213	average values	2200:2213	average values of retained particle DM and NDF weight for all sampling time points	2200:2281	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	10	108	theme	total	1547:1551	arg1	DM					1568:1569	total fecal particle DM	1547:1569	total fecal particle DM	1547:1569	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	4	109	theme	×	721:721	arg1	design					762:767	a split-plot 4 × 3 incomplete Latin square experimental design	706:767	a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling)	706:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	14	110	theme	retained	2218:2225	arg1	DM					2236:2237	retained particle DM	2218:2237	retained particle DM	2218:2237	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	3	111	from	techniques	485:494	arg1	matter					518:523	fecal particle dry matter	499:523	fecal particle dry matter (DM)	499:528	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	111	from	techniques	485:494	arg1	fiber					549:553	neutral detergent fiber	531:553	neutral detergent fiber (NDF)	531:559	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	111	from	techniques	485:494	arg1	distribution					573:584	starch distribution	566:584	starch distribution	566:584	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	111	from	techniques	485:494	arg1	DM					526:527	DM	526:527	DM	526:527	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	111	from	techniques	485:494	arg1	NDF					556:558	NDF	556:558	NDF	556:558	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	111	from	techniques	485:494	arg1	sieves					593:598	NDA sieves	589:598	NDA sieves	589:598	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	6	112	theme	gain	1075:1078	arg1	g/d					1056:1058	900 to 1,000 g/d	1043:1058	900 to 1,000 g/d of body weight gain	1043:1078	Diets were fed to allow 900 to 1,000 g/d of body weight gain and fed once daily.					
30852013	3	113	from	effects	442:448	arg1	matter					518:523	fecal particle dry matter	499:523	fecal particle dry matter (DM)	499:528	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	113	from	effects	442:448	arg1	fiber					549:553	neutral detergent fiber	531:553	neutral detergent fiber (NDF)	531:559	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	113	from	effects	442:448	arg1	distribution					573:584	starch distribution	566:584	starch distribution	566:584	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	113	from	effects	442:448	arg1	DM					526:527	DM	526:527	DM	526:527	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	113	from	effects	442:448	arg1	NDF					556:558	NDF	556:558	NDF	556:558	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	113	from	effects	442:448	arg1	sieves					593:598	NDA sieves	589:598	NDA sieves	589:598	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	6	114	theme	body	1063:1066	arg1	gain					1075:1078	body weight gain	1063:1078	body weight gain	1063:1078	Diets were fed to allow 900 to 1,000 g/d of body weight gain and fed once daily.					
30852013	5	115	theme	ADF	894:896	arg1	%					887:887	high ADF <35%	875:887	high ADF <35%	875:887	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	115	theme	ADF	894:896	arg1	%					901:901	low ADF >35%	890:901	low ADF >35%	890:901	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	7	116	theme	sampling	1182:1189	arg1	period					1191:1196	the sampling period	1178:1196	the sampling period	1178:1196	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	11	117	theme	particle	1901:1908	arg1	weight					1913:1918	the retained particle DM weight	1888:1918	the retained particle DM weight	1888:1918	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	3	118	theme	sampling	476:483	arg1	techniques					485:494	sampling techniques	476:494	sampling techniques	476:494	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	11	119	dep	decreased	1706:1714	arg1	whereas					1824:1830	whereas	1824:1830	whereas	1824:1830	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	11	120	theme	%	1853:1853	arg1	NDA					1848:1850	NDA	1848:1850	NDA (% DM)	1848:1857	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	11	120	theme	%	1853:1853	arg1	DM					1855:1856	% DM	1853:1856	% DM	1853:1856	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	2	121	dep	Analyzer	299:306	arg1	NDA					335:337	NDA	335:337	NDA	335:337	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	14	122	theme	average	2200:2206	arg1	values					2208:2213	average values	2200:2213	average values of retained particle DM and NDF weight for all sampling time points	2200:2281	However, average values of retained particle DM and NDF weight for all sampling time points were similar to ones from total daily composite sample.					
30852013	4	123	theme	18-d	774:777	arg1	periods					779:785	18-d periods	774:785	18-d periods (15 d of adaptation and 3 d of sampling)	774:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	123	theme	18-d	774:777	arg1	d					791:791	15 d	788:791	15 d of adaptation	788:805	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	123	theme	18-d	774:777	arg1	d					813:813	3 d	811:813	3 d of sampling	811:825	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	124	theme	age	637:639	arg1	mo					652:653	age 18.4 ± 0.6 mo	637:653	age 18.4 ± 0.6 mo	637:653	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	0	125	theme	particle	6:13	arg1	matter					19:24	Fecal particle dry matter	0:24	Fecal particle dry matter	0:24	Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality.					
30852013	7	126	theme	daily	1292:1296	arg1	sample					1308:1313	a daily composite sample	1290:1313	a daily composite sample	1290:1313	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	2	127	theme	wet	362:364	arg1	sieving					366:372	wet sieving	362:372	wet sieving for on-farm assessment	362:395	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	5	128	theme	restricted	999:1008	arg1	intakes					1010:1016	ad libitum and restricted intakes	984:1016	intakes	1010:1016	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	129	theme	high	923:926	arg1	%					949:949	low NDF <40%	938:949	low NDF <40%	938:949	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	5	129	theme	high	923:926	arg1	%					935:935	high NDF >45%	923:935	high NDF >45%	923:935	Treatment rations differed in forage quality (high ADF <35%, low ADF >35%) and fiber content (high NDF >45%, low NDF <40%) and were offered to heifers for ad libitum and restricted intakes.					
30852013	11	130	theme	retained	1688:1695	arg1	material					1697:1704	retained material	1688:1704	retained material	1688:1704	The NDF content in retained material decreased with decreasing sieve size (from 89.81 to 86.86%) and was not affected by forage quality and fiber content, whereas NDF retained on NDA (% DM) followed the same pattern as the retained particle DM weight.					
30852013	4	131	theme	means	625:629	arg1	SD					633:634	means ± SD	625:634	means ± SD	625:634	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	0	132	theme	fiber	30:34	arg1	distribution					36:47	fiber distribution	30:47	fiber distribution	30:47	Fecal particle dry matter and fiber distribution of heifers fed ad libitum and restricted with low and high forage quality.					
30852013	7	133	theme	collection	1266:1275	arg1	manner					1244:1249	the manner	1240:1249	the manner of total fecal collection	1240:1275	Fecal grab samples were collected 0, 6, 12, and 18 h after feeding all 3 d of the sampling period, and the remaining feces was collected in the manner of total fecal collection to represent a daily composite sample.					
30852013	4	134	theme	adaptation	796:805	arg1	periods					779:785	18-d periods	774:785	18-d periods (15 d of adaptation and 3 d of sampling)	774:826	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	134	theme	adaptation	796:805	arg1	d					813:813	3 d	811:813	3 d of sampling	811:825	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	4	134	theme	adaptation	796:805	arg1	d					791:791	15 d	788:791	15 d of adaptation	788:805	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	3	135	theme	neutral	531:537	arg1	fiber					549:553	neutral detergent fiber	531:553	neutral detergent fiber (NDF)	531:559	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	3	135	theme	neutral	531:537	arg1	NDF					556:558	NDF	556:558	NDF	556:558	The objective of this study was to evaluate effects of diet manipulations and sampling techniques on fecal particle dry matter (DM), neutral detergent fiber (NDF), and starch distribution on NDA sieves.					
30852013	12	136	from	content	1928:1934	arg1	material					1948:1955	retained material	1939:1955	retained material	1939:1955	Starch content in retained material was below 0.5% in the majority of diets.					
30852013	2	137	theme	Nasco	283:287	arg1	WI					331:332	WI	331:332	WI	331:332	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	2	137	theme	Nasco	283:287	arg1	Analyzer					299:306	Nasco Digestion Analyzer	283:306	Nasco Digestion Analyzer	283:306	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	4	138	theme	±	646:646	arg1	mo					652:653	age 18.4 ± 0.6 mo	637:653	age 18.4 ± 0.6 mo	637:653	Eight Holstein heifers (means ± SD; age 18.4 ± 0.6 mo and BW 457.2 ± 27.3 kg), were randomly assigned to a split-plot 4 × 3 incomplete Latin square experimental design with 18-d periods (15 d of adaptation and 3 d of sampling).					
30852013	10	139	theme	fiber	1617:1621	arg1	content					1623:1629	high fiber content	1612:1629	high fiber content	1612:1629	Distributions of total fecal particle DM were greater for high forage quality and high fiber content diets (14.66 to 20.37% of sample DM).					
30852013	2	140	theme	sieving	366:372	arg1	principles					348:357	principles	348:357	principles of wet sieving for on-farm assessment	348:395	A commercially available system for this analysis (Nasco Digestion Analyzer, Nasco, Fort Atkinson, WI; NDA) employs principles of wet sieving for on-farm assessment.					
30852013	13	141	theme	daily	2161:2165	arg1	sample					2183:2188	a daily total composite sample	2159:2188	a daily total composite sample	2159:2188	Average retained fecal particle DM and NDF content varied depending on the time after feeding, resulting in the large differences between grab fecal samples and a daily total composite sample.					
31786303	7	0	from	HCT-116	1023:1029	arg1	%					1039:1039	73.8%	1035:1039	73.8% from A-546	1035:1050	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	7	0	from	HCT-116	1023:1029	arg1	%					1016:1016	67.6%	1012:1016	67.6% from HCT-116	1012:1029	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	7	0	from	HCT-116	1023:1029	arg1	cells					1001:1005	the dead cells	992:1005	the dead cells	992:1005	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	5	1	theme	NPs	653:655	arg1	%					690:690	21.6%	686:690	21.6%	686:690	The NPs loading capacity for CUR was 21.6% and the encapsulation efficiency reached 83.8%.					
31786303	5	1	theme	NPs	653:655	arg1	capacity					665:672	The NPs loading capacity	649:672	The NPs loading capacity for CUR	649:680	The NPs loading capacity for CUR was 21.6% and the encapsulation efficiency reached 83.8%.					
31786303	1	2	theme	deacetylation	137:149	arg1	degree					151:156	88.7% deacetylation degree	131:156	88.7% deacetylation degree	131:156	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	8	3	with	treatment	1163:1171	arg1	150 μM					1178:1183	150 μM	1178:1183	150 μM of CUR/FCt-NPs for 72 h	1178:1207	Fluorescent imaging indicated that most cancer cells entered the apoptosis phase after treatment with 150 μM of CUR/FCt-NPs for 72 h.					
31786303	5	4	theme	loading	657:663	arg1	%					690:690	21.6%	686:690	21.6%	686:690	The NPs loading capacity for CUR was 21.6% and the encapsulation efficiency reached 83.8%.					
31786303	5	4	theme	loading	657:663	arg1	capacity					665:672	The NPs loading capacity	649:672	The NPs loading capacity for CUR	649:680	The NPs loading capacity for CUR was 21.6% and the encapsulation efficiency reached 83.8%.					
31786303	4	5	theme	average	574:580	arg1	diameters					582:590	average diameters	574:590	average diameters of 115 ± 21 nm	574:605	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	8	6	theme	Fluorescent	1076:1086	arg1	imaging					1088:1094	Fluorescent imaging	1076:1094	Fluorescent imaging	1076:1094	Fluorescent imaging indicated that most cancer cells entered the apoptosis phase after treatment with 150 μM of CUR/FCt-NPs for 72 h.					
31786303	4	7	theme	CUR/FCt-NPs	497:507	arg1	micrographs					470:480	The scanning micrographs	457:480	The scanning micrographs of synthesized CUR/FCt-NPs	457:507	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	1	8	theme	ionic	241:245	arg1	gelation					247:254	ionic gelation	241:254	ionic gelation	241:254	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	9	9	theme	FCt-NPs	1228:1234	arg1	efficiency					1214:1223	The efficiency	1210:1223	The efficiency of FCt-NPs	1210:1234	The efficiency of FCt-NPs was proved as carriers for loading CUR and augmenting its anticancer activity toward human cancer cells, using these natural and biosafe agents.					
31786303	8	10	theme	CUR/FCt-NPs	1188:1198	arg1	150 μM					1178:1183	150 μM	1178:1183	150 μM of CUR/FCt-NPs for 72 h	1178:1207	Fluorescent imaging indicated that most cancer cells entered the apoptosis phase after treatment with 150 μM of CUR/FCt-NPs for 72 h.					
31786303	1	11	theme	Fungal	80:85	arg1	FCt					97:99	FCt	97:99	FCt	97:99	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	1	11	theme	Fungal	80:85	arg1	chitosan					87:94	Fungal chitosan	80:94	Fungal chitosan (FCt)	80:100	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	9	12	theme	biosafe	1365:1371	arg1	agents					1373:1378	these natural and biosafe agents	1347:1378	these natural and biosafe agents	1347:1378	The efficiency of FCt-NPs was proved as carriers for loading CUR and augmenting its anticancer activity toward human cancer cells, using these natural and biosafe agents.					
31786303	9	13	theme	natural	1353:1359	arg1	agents					1373:1378	these natural and biosafe agents	1347:1378	these natural and biosafe agents	1347:1378	The efficiency of FCt-NPs was proved as carriers for loading CUR and augmenting its anticancer activity toward human cancer cells, using these natural and biosafe agents.					
31786303	8	14	theme	apoptosis	1141:1149	arg1	phase					1151:1155	the apoptosis phase	1137:1155	the apoptosis phase	1137:1155	Fluorescent imaging indicated that most cancer cells entered the apoptosis phase after treatment with 150 μM of CUR/FCt-NPs for 72 h.					
31786303	7	15	with	treatment	875:883	arg1	NPs					934:936	CUR/FCt NPs	926:936	CUR/FCt NPs	926:936	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	6	16	theme	beginning	775:783	arg1	5 h					785:787	the beginning 5 h	771:787	the beginning 5 h then gradually released up to 90 h	771:822	The CUR was vastly released in the beginning 5 h then gradually released up to 90 h, with higher release in pH 5.2 than in pH 7.0.					
31786303	1	17	theme	112.4 kDa	162:170	arg1	weight					182:187	112.4 kDa molecular weight	162:187	112.4 kDa molecular weight	162:187	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	0	18	theme	anticancer	10:19	arg1	activity					21:28	Augmented anticancer activity	0:28	Augmented anticancer activity of curcumin	0:40	Augmented anticancer activity of curcumin loaded fungal chitosan nanoparticles.					
31786303	4	19	theme	scanning	461:468	arg1	micrographs					470:480	The scanning micrographs	457:480	The scanning micrographs of synthesized CUR/FCt-NPs	457:507	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	1	20	theme	molecular	172:180	arg1	weight					182:187	112.4 kDa molecular weight	162:187	112.4 kDa molecular weight	162:187	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	0	21	theme	Augmented	0:8	arg1	activity					21:28	Augmented anticancer activity	0:28	Augmented anticancer activity of curcumin	0:40	Augmented anticancer activity of curcumin loaded fungal chitosan nanoparticles.					
31786303	7	22	theme	exposure	1066:1073	arg1	96 h					1058:1061	96 h	1058:1061	96 h of exposure	1058:1073	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	7	23	theme	cancer	888:893	arg1	A-549					914:918	A-549	914:918	A-549	914:918	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	7	23	theme	cancer	888:893	arg1	HCT-116					902:908	HCT-116	902:908	HCT-116	902:908	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	7	23	theme	cancer	888:893	arg1	cells					895:899	cancer cells	888:899	cancer cells	888:899	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	4	24	contain	had	570:572	arg2	potentials					625:634	positive zeta potentials	611:634	positive zeta potentials of +33.8 mV	611:646	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	4	24	contain	had	570:572	arg1	they					565:568	they	565:568	they	565:568	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	4	24	contain	had	570:572	arg2	diameters					582:590	average diameters	574:590	average diameters of 115 ± 21 nm	574:605	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	2	25	theme	anticancer	342:351	arg1	bioactivity					353:363	anticancer bioactivity	342:363	anticancer bioactivity	342:363	FCt-NPs were employed as carriers for curcumin (CUR) to augment its availability and anticancer bioactivity.					
31786303	1	26	from	rouxii	118:123	arg1	FCt					97:99	FCt	97:99	FCt	97:99	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	1	26	from	rouxii	118:123	arg1	chitosan					87:94	Fungal chitosan	80:94	Fungal chitosan (FCt)	80:100	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	9	27	theme	anticancer	1294:1303	arg1	activity					1305:1312	its anticancer activity	1290:1312	its anticancer activity toward human cancer cells	1290:1338	The efficiency of FCt-NPs was proved as carriers for loading CUR and augmenting its anticancer activity toward human cancer cells, using these natural and biosafe agents.					
31786303	9	27	theme	anticancer	1294:1303	arg1	carriers					1250:1257	carriers	1250:1257	carriers for loading CUR	1250:1273	The efficiency of FCt-NPs was proved as carriers for loading CUR and augmenting its anticancer activity toward human cancer cells, using these natural and biosafe agents.					
31786303	3	28	theme	FTIR	443:446	arg1	spectra					448:454	their FTIR spectra	437:454	their FTIR spectra	437:454	The synthesis of CUR/FCt-NPs composite was succeeded as evidenced from their FTIR spectra.					
31786303	6	29	from	release	837:843	arg1	pH 5.2					848:853	pH 5.2	848:853	pH 5.2	848:853	The CUR was vastly released in the beginning 5 h then gradually released up to 90 h, with higher release in pH 5.2 than in pH 7.0.					
31786303	6	29	from	release	837:843	arg1	pH 7.0					863:868	pH 7.0	863:868	pH 7.0	863:868	The CUR was vastly released in the beginning 5 h then gradually released up to 90 h, with higher release in pH 5.2 than in pH 7.0.					
31786303	7	30	theme	dead	996:999	arg1	cells					1001:1005	the dead cells	992:1005	the dead cells	992:1005	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	7	30	theme	dead	996:999	arg1	%					1016:1016	67.6%	1012:1016	67.6% from HCT-116	1012:1029	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	4	31	theme	positive	611:618	arg1	potentials					625:634	positive zeta potentials	611:634	positive zeta potentials of +33.8 mV	611:646	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	3	32	theme	CUR/FCt-NPs	383:393	arg1	composite					395:403	CUR/FCt-NPs composite	383:403	CUR/FCt-NPs composite	383:403	The synthesis of CUR/FCt-NPs composite was succeeded as evidenced from their FTIR spectra.					
31786303	5	33	theme	encapsulation	700:712	arg1	efficiency					714:723	the encapsulation efficiency	696:723	the encapsulation efficiency	696:723	The NPs loading capacity for CUR was 21.6% and the encapsulation efficiency reached 83.8%.					
31786303	8	34	theme	most	1111:1114	arg1	cells					1123:1127	most cancer cells	1111:1127	most cancer cells	1111:1127	Fluorescent imaging indicated that most cancer cells entered the apoptosis phase after treatment with 150 μM of CUR/FCt-NPs for 72 h.					
31786303	9	35	theme	loading	1263:1269	arg1	CUR					1271:1273	loading CUR	1263:1273	loading CUR	1263:1273	The efficiency of FCt-NPs was proved as carriers for loading CUR and augmenting its anticancer activity toward human cancer cells, using these natural and biosafe agents.					
31786303	3	36	theme	composite	395:403	arg1	synthesis					370:378	The synthesis	366:378	The synthesis of CUR/FCt-NPs composite	366:403	The synthesis of CUR/FCt-NPs composite was succeeded as evidenced from their FTIR spectra.					
31786303	4	37	theme	spherical	525:533	arg1	shapes					535:540	their spherical shapes	519:540	their spherical shapes	519:540	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	0	38	theme	curcumin	33:40	arg1	activity					21:28	Augmented anticancer activity	0:28	Augmented anticancer activity of curcumin	0:40	Augmented anticancer activity of curcumin loaded fungal chitosan nanoparticles.					
31786303	6	39	theme	higher	830:835	arg1	release					837:843	higher release	830:843	higher release in pH 5.2 than in pH 7.0	830:868	The CUR was vastly released in the beginning 5 h then gradually released up to 90 h, with higher release in pH 5.2 than in pH 7.0.					
31786303	1	40	used	utilized	194:201	arg2	FCt					97:99	FCt	97:99	FCt	97:99	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	1	40	used	utilized	194:201	arg2	chitosan					87:94	Fungal chitosan	80:94	Fungal chitosan (FCt)	80:100	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	7	41	from	A-546	1046:1050	arg1	%					1039:1039	73.8%	1035:1039	73.8% from A-546	1035:1050	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	7	41	from	A-546	1046:1050	arg1	%					1016:1016	67.6%	1012:1016	67.6% from HCT-116	1012:1029	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	7	41	from	A-546	1046:1050	arg1	cells					1001:1005	the dead cells	992:1005	the dead cells	992:1005	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	0	42	theme	chitosan	56:63	arg1	nanoparticles					65:77	fungal chitosan nanoparticles	49:77	fungal chitosan nanoparticles	49:77	Augmented anticancer activity of curcumin loaded fungal chitosan nanoparticles.					
31786303	1	43	with	chitosan	87:94	arg1	weight					182:187	112.4 kDa molecular weight	162:187	112.4 kDa molecular weight	162:187	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	1	43	with	chitosan	87:94	arg1	degree					151:156	88.7% deacetylation degree	131:156	88.7% deacetylation degree	131:156	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	1	44	theme	Amylomyces	107:116	arg1	rouxii					118:123	Amylomyces rouxii	107:123	Amylomyces rouxii	107:123	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	0	45	theme	fungal	49:54	arg1	nanoparticles					65:77	fungal chitosan nanoparticles	49:77	fungal chitosan nanoparticles	49:77	Augmented anticancer activity of curcumin loaded fungal chitosan nanoparticles.					
31786303	7	46	theme	time-dependent	946:959	arg1	decrement					961:969	time-dependent decrement	946:969	time-dependent decrement of cells' viability	946:989	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	7	47	theme	cells	895:899	arg1	treatment					875:883	The treatment	871:883	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs	871:936	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	1	48	theme	nanoparticles	207:219	arg1	formation					227:235	nanoparticles (NPs) formation	207:235	nanoparticles (NPs) formation	207:235	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	7	49	theme	viability	981:989	arg1	decrement					961:969	time-dependent decrement	946:969	time-dependent decrement of cells' viability	946:989	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	4	50	theme	+33.8 mV	639:646	arg1	diameters					582:590	average diameters	574:590	average diameters of 115 ± 21 nm	574:605	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	4	50	theme	+33.8 mV	639:646	arg1	potentials					625:634	positive zeta potentials	611:634	positive zeta potentials of +33.8 mV	611:646	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	1	51	theme	88.7	131:134	arg1	%					135:135	%	135:135	%	135:135	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	9	52	theme	human	1321:1325	arg1	cells					1334:1338	human cancer cells	1321:1338	human cancer cells	1321:1338	The efficiency of FCt-NPs was proved as carriers for loading CUR and augmenting its anticancer activity toward human cancer cells, using these natural and biosafe agents.					
31786303	4	53	theme	115 ± 21 nm	595:605	arg1	diameters					582:590	average diameters	574:590	average diameters of 115 ± 21 nm	574:605	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	4	53	theme	115 ± 21 nm	595:605	arg1	potentials					625:634	positive zeta potentials	611:634	positive zeta potentials of +33.8 mV	611:646	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	4	54	theme	synthesized	485:495	arg1	CUR/FCt-NPs					497:507	synthesized CUR/FCt-NPs	485:507	synthesized CUR/FCt-NPs	485:507	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31786303	8	55	theme	cancer	1116:1121	arg1	cells					1123:1127	most cancer cells	1111:1127	most cancer cells	1111:1127	Fluorescent imaging indicated that most cancer cells entered the apoptosis phase after treatment with 150 μM of CUR/FCt-NPs for 72 h.					
31786303	7	56	theme	CUR/FCt	926:932	arg1	NPs					934:936	CUR/FCt NPs	926:936	CUR/FCt NPs	926:936	The treatment of cancer cells, HCT-116 and A-549, with CUR/FCt NPs lead to time-dependent decrement of cells' viability; the dead cells were 67.6% from HCT-116 and 73.8% from A-546 after 96 h of exposure.					
31786303	1	57	theme	%	135:135	arg1	degree					151:156	88.7% deacetylation degree	131:156	88.7% deacetylation degree	131:156	Fungal chitosan (FCt) from Amylomyces rouxii, with 88.7% deacetylation degree and 112.4 kDa molecular weight, was utilized for nanoparticles (NPs) formation via ionic gelation.					
31786303	9	58	theme	cancer	1327:1332	arg1	cells					1334:1338	human cancer cells	1321:1338	human cancer cells	1321:1338	The efficiency of FCt-NPs was proved as carriers for loading CUR and augmenting its anticancer activity toward human cancer cells, using these natural and biosafe agents.					
31786303	4	59	theme	zeta	620:623	arg1	potentials					625:634	positive zeta potentials	611:634	positive zeta potentials of +33.8 mV	611:646	The scanning micrographs of synthesized CUR/FCt-NPs indicated their spherical shapes and well-distribution; they had average diameters of 115 ± 21 nm and positive zeta potentials of +33.8 mV.					
31055161	3	0	theme	composite	779:787	arg1	coatings					789:796	edible composite coatings	772:796	edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts	772:905	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	5	1	theme	control	1634:1640	arg1	fruit					1642:1646	uncoated control fruit	1625:1646	uncoated control fruit	1625:1646	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	2	theme	weight	1418:1423	arg1	loss					1425:1428	weight loss	1418:1428	weight loss	1418:1428	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	3	from	5 °C	1320:1323	arg1	periods					1294:1300	periods	1294:1300	periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C	1294:1362	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	0	4	theme	postharvest	206:216	arg1	quality					218:224	postharvest quality	206:224	postharvest quality of citrus fruit	206:240	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	5	5	contain	containing	1374:1383	arg2	SB					1393:1394	SB	1393:1394	SB	1393:1394	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	5	contain	containing	1374:1383	arg2	SEP					1385:1387	SEP	1385:1387	SEP	1385:1387	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	5	contain	containing	1374:1383	arg1	coatings					1365:1372	coatings	1365:1372	coatings containing SEP and SB	1365:1394	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	3	6	theme	hydroxypropyl	814:826	arg1	methylcellulose					828:842	hydroxypropyl methylcellulose	814:842	hydroxypropyl methylcellulose (HPMC)	814:849	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	3	6	theme	hydroxypropyl	814:826	arg1	HPMC					845:848	HPMC	845:848	HPMC	845:848	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	4	7	theme	2	1078:1078	arg1	%					1079:1079	%	1079:1079	%	1079:1079	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	2	8	dep	carbonate	556:564	arg1	%					574:574	0.2%	571:574	0.2%	571:574	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	8	dep	carbonate	556:564	arg1	PC					567:568	PC	567:568	PC	567:568	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	4	9	dep	severity	1125:1132	arg1	reduction					1145:1153	up to 50% reduction	1135:1153	up to 50% reduction	1135:1153	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	2	10	dep	benzoate	652:659	arg1	SB					662:663	SB	662:663	SB	662:663	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	10	dep	benzoate	652:659	arg1	%					669:669	2.0%	666:669	2.0%	666:669	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	0	11	theme	fruit	236:240	arg1	quality					218:224	postharvest quality	206:224	postharvest quality of citrus fruit	206:240	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	1	12	theme	Diplodia	462:469	arg1	rot					480:482	citrus Diplodia stem-end rot	455:482	citrus Diplodia stem-end rot	455:482	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	3	13	with	experiments	931:941	arg1	oranges					986:992	'Barnfield' oranges	974:992	'Barnfield' oranges	974:992	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	3	13	with	experiments	931:941	arg1	mandarins					960:968	'Ortanique' mandarins	948:968	'Ortanique' mandarins	948:968	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	2	14	theme	potassium	516:524	arg1	sorbate					526:532	potassium sorbate	516:532	potassium sorbate (PS, 2.0%)	516:543	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	5	15	theme	shelf	1344:1348	arg1	life					1350:1353	shelf life	1344:1353	shelf life	1344:1353	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	0	16	theme	composite	134:142	arg1	coatings					151:158	hydroxypropyl methylcellulose-lipid composite edible coatings	98:158	hydroxypropyl methylcellulose-lipid composite edible coatings	98:158	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	1	17	theme	growth	379:384	arg1	inhibition					356:365	inhibition	356:365	inhibition of mycelial growth on PDA dishes	356:398	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	5	18	theme	physicochemical	1463:1477	arg1	content					1523:1529	soluble solid content	1509:1529	soluble solid content	1509:1529	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	18	theme	physicochemical	1463:1477	arg1	ethanol					1556:1562	ethanol	1556:1562	ethanol	1556:1562	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	18	theme	physicochemical	1463:1477	arg1	attributes					1487:1496	the physicochemical quality attributes	1459:1496	the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content)	1459:1588	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	18	theme	physicochemical	1463:1477	arg1	firmness					1499:1506	firmness	1499:1506	firmness	1499:1506	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	18	theme	physicochemical	1463:1477	arg1	acidity					1543:1549	titratable acidity	1532:1549	titratable acidity	1532:1549	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	18	theme	physicochemical	1463:1477	arg1	content					1581:1587	acetaldehyde content	1568:1587	acetaldehyde content	1568:1587	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	4	19	theme	effective	1098:1106	arg1	Coatings					1038:1045	Coatings	1038:1045	Coatings containing 2% PS, 0.1% SEP, or 2% SB	1038:1082	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	4	19	theme	effective	1098:1106	arg1	severity					1125:1132	the most effective reducing disease severity	1089:1132	the most effective reducing disease severity	1089:1132	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	0	20	theme	coatings	151:158	arg1	activity					11:18	Antifungal activity	0:18	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro	0:74	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	0	20	theme	coatings	151:158	arg1	ingredients					83:93	as ingredients	80:93	as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings	80:158	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	1	21	theme	PDA	389:391	arg1	dishes					393:398	PDA dishes	389:398	PDA dishes	389:398	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	5	22	theme	titratable	1532:1541	arg1	attributes					1487:1496	the physicochemical quality attributes	1459:1496	the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content)	1459:1588	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	22	theme	titratable	1532:1541	arg1	acidity					1543:1549	titratable acidity	1532:1549	titratable acidity	1532:1549	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	4	23	dep	non-inoculated	1181:1194	arg1	oranges					1224:1230	oranges	1224:1230	oranges	1224:1230	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	4	24	theme	disease	1117:1123	arg1	Coatings					1038:1045	Coatings	1038:1045	Coatings containing 2% PS, 0.1% SEP, or 2% SB	1038:1082	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	4	24	theme	disease	1117:1123	arg1	severity					1125:1132	the most effective reducing disease severity	1089:1132	the most effective reducing disease severity	1089:1132	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	1	25	dep	recognized	277:286	arg1	generally					267:275	generally	267:275	generally	267:275	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	0	26	theme	stem-end	180:187	arg1	rot					189:191	Diplodia stem-end rot	171:191	Diplodia stem-end rot	171:191	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	5	27	theme	soluble	1509:1515	arg1	attributes					1487:1496	the physicochemical quality attributes	1459:1496	the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content)	1459:1588	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	27	theme	soluble	1509:1515	arg1	content					1523:1529	soluble solid content	1509:1529	soluble solid content	1509:1529	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	2	28	theme	sodium	612:617	arg1	ethylparaben					619:630	sodium ethylparaben	612:630	sodium ethylparaben (SEP, 0.1%)	612:642	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	4	29	theme	%	1143:1143	arg1	reduction					1145:1153	up to 50% reduction	1135:1153	up to 50% reduction	1135:1153	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	1	30	theme	causal	439:444	arg1	agent					446:450	the causal agent	435:450	the causal agent of citrus Diplodia stem-end rot	435:482	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	30	theme	causal	439:444	arg1	theobromae					423:432	Lasiodiplodia theobromae	409:432	Lasiodiplodia theobromae	409:432	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	31	from	dishes	393:398	arg1	inhibition					356:365	inhibition	356:365	inhibition of mycelial growth on PDA dishes	356:398	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	0	32	theme	hydroxypropyl	98:110	arg1	coatings					151:158	hydroxypropyl methylcellulose-lipid composite edible coatings	98:158	hydroxypropyl methylcellulose-lipid composite edible coatings	98:158	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	3	33	theme	antifungal	885:894	arg1	salts					901:905	these selected antifungal GRAS salts	870:905	these selected antifungal GRAS salts	870:905	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	2	34	dep	ethylparaben	619:630	arg1	SEP					633:635	SEP	633:635	SEP	633:635	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	34	dep	ethylparaben	619:630	arg1	%					641:641	0.1%	638:641	0.1%	638:641	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	6	35	theme	gas	1671:1673	arg1	concentration					1675:1687	the internal gas concentration	1658:1687	the internal gas concentration (CO2 level) of coated fruit	1658:1715	Although the internal gas concentration (CO2 level) of coated fruit increased, the coatings did not induce off-flavors.					
31055161	6	35	theme	gas	1671:1673	arg1	level					1694:1698	CO2 level	1690:1698	CO2 level	1690:1698	Although the internal gas concentration (CO2 level) of coated fruit increased, the coatings did not induce off-flavors.					
31055161	0	36	theme	Antifungal	0:9	arg1	activity					11:18	Antifungal activity	0:18	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro	0:74	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	1	37	dep	tests	349:353	arg1	inhibition					356:365	inhibition	356:365	inhibition of mycelial growth on PDA dishes	356:398	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	5	38	theme	life	1350:1353	arg1	d					1339:1339	7 d	1337:1339	7 d of shelf life at 20 °C	1337:1362	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	1	39	from	inhibition	356:365	arg1	dishes					393:398	PDA dishes	389:398	PDA dishes	389:398	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	40	dep	in	340:341	arg1	vitro					343:347	vitro	343:347	vitro	343:347	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	0	41	theme	salts	28:32	arg1	activity					11:18	Antifungal activity	0:18	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro	0:74	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	0	41	theme	salts	28:32	arg1	ingredients					83:93	as ingredients	80:93	as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings	80:158	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	5	42	from	20 °C	1358:1362	arg1	d					1339:1339	7 d	1337:1339	7 d of shelf life at 20 °C	1337:1362	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	4	43	theme	0.1	1065:1067	arg1	%					1068:1068	%	1068:1068	%	1068:1068	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	4	44	theme	postharvest	1261:1271	arg1	quality					1279:1285	postharvest fruit quality	1261:1285	postharvest fruit quality	1261:1285	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	3	45	theme	Disease	745:751	arg1	ability					761:767	Disease control ability	745:767	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts	745:905	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	6	46	theme	fruit	1711:1715	arg1	concentration					1675:1687	the internal gas concentration	1658:1687	the internal gas concentration (CO2 level) of coated fruit	1658:1715	Although the internal gas concentration (CO2 level) of coated fruit increased, the coatings did not induce off-flavors.					
31055161	6	46	theme	fruit	1711:1715	arg1	level					1694:1698	CO2 level	1690:1698	CO2 level	1690:1698	Although the internal gas concentration (CO2 level) of coated fruit increased, the coatings did not induce off-flavors.					
31055161	3	47	theme	in	923:924	arg1	experiments					931:941	in vivo experiments	923:941	in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae	923:1035	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	3	48	theme	coatings	789:796	arg1	ability					761:767	Disease control ability	745:767	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts	745:905	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	5	49	dep	attributes	1487:1496	arg1	content					1523:1529	soluble solid content	1509:1529	soluble solid content	1509:1529	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	49	dep	attributes	1487:1496	arg1	ethanol					1556:1562	ethanol	1556:1562	ethanol	1556:1562	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	49	dep	attributes	1487:1496	arg1	attributes					1487:1496	the physicochemical quality attributes	1459:1496	the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content)	1459:1588	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	49	dep	attributes	1487:1496	arg1	firmness					1499:1506	firmness	1499:1506	firmness	1499:1506	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	49	dep	attributes	1487:1496	arg1	acidity					1543:1549	titratable acidity	1532:1549	titratable acidity	1532:1549	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	49	dep	attributes	1487:1496	arg1	content					1581:1587	acetaldehyde content	1568:1587	acetaldehyde content	1568:1587	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	50	theme	21	1305:1306	arg1	d					1315:1315	21 and 42 d	1305:1315	d	1315:1315	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	51	theme	acetaldehyde	1568:1579	arg1	attributes					1487:1496	the physicochemical quality attributes	1459:1496	the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content)	1459:1588	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	51	theme	acetaldehyde	1568:1579	arg1	content					1581:1587	acetaldehyde content	1568:1587	acetaldehyde content	1568:1587	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	2	52	dep	sorbate	526:532	arg1	PS					535:536	PS	535:536	PS	535:536	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	52	dep	sorbate	526:532	arg1	%					542:542	2.0%	539:542	2.0%	539:542	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	3	53	theme	edible	772:777	arg1	coatings					789:796	edible composite coatings	772:796	edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts	772:905	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	5	54	theme	42	1312:1313	arg1	d					1315:1315	21 and 42 d	1305:1315	d	1315:1315	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	4	55	theme	%	1079:1079	arg1	SB					1081:1082	2% SB	1078:1082	2% SB	1078:1082	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	1	56	theme	GRAS	261:264	arg1	salts					297:301	GRAS (generally recognized as safe) salts	261:301	GRAS (generally recognized as safe) salts	261:301	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	5	57	theme	sensory	1594:1600	arg1	flavor					1602:1607	sensory flavor	1594:1607	sensory flavor with respect to uncoated control fruit	1594:1646	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	58	theme	quality	1479:1485	arg1	content					1523:1529	soluble solid content	1509:1529	soluble solid content	1509:1529	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	58	theme	quality	1479:1485	arg1	ethanol					1556:1562	ethanol	1556:1562	ethanol	1556:1562	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	58	theme	quality	1479:1485	arg1	attributes					1487:1496	the physicochemical quality attributes	1459:1496	the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content)	1459:1588	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	58	theme	quality	1479:1485	arg1	firmness					1499:1506	firmness	1499:1506	firmness	1499:1506	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	58	theme	quality	1479:1485	arg1	acidity					1543:1549	titratable acidity	1532:1549	titratable acidity	1532:1549	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	58	theme	quality	1479:1485	arg1	content					1581:1587	acetaldehyde content	1568:1587	acetaldehyde content	1568:1587	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	3	59	theme	Barnfield	975:983	arg1	oranges					986:992	'Barnfield' oranges	974:992	'Barnfield' oranges	974:992	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	0	60	theme	citrus	229:234	arg1	fruit					236:240	citrus fruit	229:240	citrus fruit	229:240	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	1	61	theme	citrus	455:460	arg1	rot					480:482	citrus Diplodia stem-end rot	455:482	citrus Diplodia stem-end rot	455:482	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	2	62	theme	potassium	546:554	arg1	carbonate					556:564	potassium carbonate	546:564	potassium carbonate (PC, 0.2%)	546:575	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	63	theme	sodium	645:650	arg1	benzoate					652:659	sodium benzoate	645:659	sodium benzoate (SB, 2.0%)	645:670	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	4	64	contain	containing	1047:1056	arg2	SEP					1070:1072	0.1% SEP	1065:1072	0.1% SEP	1065:1072	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	4	64	contain	containing	1047:1056	arg1	Coatings					1038:1045	Coatings	1038:1045	Coatings containing 2% PS, 0.1% SEP, or 2% SB	1038:1082	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	4	64	contain	containing	1047:1056	arg2	SB					1081:1082	2% SB	1078:1082	2% SB	1078:1082	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	4	64	contain	containing	1047:1056	arg2	PS					1061:1062	2% PS	1058:1062	2% PS	1058:1062	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	4	64	contain	containing	1047:1056	arg1	severity					1125:1132	the most effective reducing disease severity	1089:1132	the most effective reducing disease severity	1089:1132	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	5	65	theme	d	1315:1315	arg1	periods					1294:1300	periods	1294:1300	periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C	1294:1362	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	0	66	theme	edible	144:149	arg1	coatings					151:158	hydroxypropyl methylcellulose-lipid composite edible coatings	98:158	hydroxypropyl methylcellulose-lipid composite edible coatings	98:158	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	1	67	theme	stem-end	471:478	arg1	rot					480:482	citrus Diplodia stem-end rot	455:482	citrus Diplodia stem-end rot	455:482	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	0	68	theme	Diplodia	171:178	arg1	rot					189:191	Diplodia stem-end rot	171:191	Diplodia stem-end rot	171:191	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	4	69	theme	reducing	1108:1115	arg1	Coatings					1038:1045	Coatings	1038:1045	Coatings containing 2% PS, 0.1% SEP, or 2% SB	1038:1082	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	4	69	theme	reducing	1108:1115	arg1	severity					1125:1132	the most effective reducing disease severity	1089:1132	the most effective reducing disease severity	1089:1132	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	1	70	dep	GRAS	261:264	arg1	recognized					277:286	recognized	277:286	recognized as safe	277:294	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	5	71	theme	solid	1517:1521	arg1	attributes					1487:1496	the physicochemical quality attributes	1459:1496	the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content)	1459:1588	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	5	71	theme	solid	1517:1521	arg1	content					1523:1529	soluble solid content	1509:1529	soluble solid content	1509:1529	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31055161	2	72	dep	methylparaben	585:597	arg1	SMP					600:602	SMP	600:602	SMP	600:602	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	72	dep	methylparaben	585:597	arg1	%					608:608	0.1%	605:608	0.1%	605:608	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	73	theme	Ammonium	485:492	arg1	carbonate					494:502	Ammonium carbonate	485:502	Ammonium carbonate (AC, 0.2%)	485:513	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	4	74	from	effect	1251:1256	arg1	quality					1279:1285	postharvest fruit quality	1261:1285	postharvest fruit quality	1261:1285	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	3	75	dep	in	923:924	arg1	vivo					926:929	vivo	926:929	vivo	926:929	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	0	76	theme	as	80:81	arg1	ingredients					83:93	as ingredients	80:93	as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings	80:158	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	1	77	theme	in	340:341	arg1	tests					349:353	in vitro tests	340:353	in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot	340:482	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	6	78	theme	CO2	1690:1692	arg1	concentration					1675:1687	the internal gas concentration	1658:1687	the internal gas concentration (CO2 level) of coated fruit	1658:1715	Although the internal gas concentration (CO2 level) of coated fruit increased, the coatings did not induce off-flavors.					
31055161	6	78	theme	CO2	1690:1692	arg1	level					1694:1698	CO2 level	1690:1698	CO2 level	1690:1698	Although the internal gas concentration (CO2 level) of coated fruit increased, the coatings did not induce off-flavors.					
31055161	0	79	theme	methylcellulose-lipid	112:132	arg1	coatings					151:158	hydroxypropyl methylcellulose-lipid composite edible coatings	98:158	hydroxypropyl methylcellulose-lipid composite edible coatings	98:158	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	3	80	theme	selected	876:883	arg1	salts					901:905	these selected antifungal GRAS salts	870:905	these selected antifungal GRAS salts	870:905	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	1	81	from	growth	379:384	arg1	dishes					393:398	PDA dishes	389:398	PDA dishes	389:398	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	82	theme	mycelial	370:377	arg1	growth					379:384	mycelial growth	370:384	mycelial growth on PDA dishes	370:398	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	4	83	theme	2	1058:1058	arg1	%					1059:1059	%	1059:1059	%	1059:1059	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	2	84	theme	sodium	578:583	arg1	methylparaben					585:597	sodium methylparaben	578:597	sodium methylparaben (SMP, 0.1%)	578:609	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	6	85	theme	internal	1662:1669	arg1	concentration					1675:1687	the internal gas concentration	1658:1687	the internal gas concentration (CO2 level) of coated fruit	1658:1715	Although the internal gas concentration (CO2 level) of coated fruit increased, the coatings did not induce off-flavors.					
31055161	6	85	theme	internal	1662:1669	arg1	level					1694:1698	CO2 level	1690:1698	CO2 level	1690:1698	Although the internal gas concentration (CO2 level) of coated fruit increased, the coatings did not induce off-flavors.					
31055161	0	86	theme	GRAS	23:26	arg1	salts					28:32	GRAS salts	23:32	GRAS salts	23:32	Antifungal activity of GRAS salts against Lasiodiplodia theobromae in vitro and as ingredients of hydroxypropyl methylcellulose-lipid composite edible coatings to control Diplodia stem-end rot and maintain postharvest quality of citrus fruit.					
31055161	1	87	theme	salts	297:301	arg1	amount					251:256	A large amount	243:256	A large amount of GRAS (generally recognized as safe) salts and concentrations	243:320	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	87	theme	salts	297:301	arg1	concentrations					307:320	concentrations	307:320	concentrations	307:320	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	87	theme	salts	297:301	arg1	salts					297:301	GRAS (generally recognized as safe) salts	261:301	GRAS (generally recognized as safe) salts	261:301	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	3	88	theme	GRAS	896:899	arg1	salts					901:905	these selected antifungal GRAS salts	870:905	these selected antifungal GRAS salts	870:905	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	4	89	theme	%	1068:1068	arg1	SEP					1070:1072	0.1% SEP	1065:1072	0.1% SEP	1065:1072	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	1	90	theme	rot	480:482	arg1	agent					446:450	the causal agent	435:450	the causal agent of citrus Diplodia stem-end rot	435:482	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	90	theme	rot	480:482	arg1	theobromae					423:432	Lasiodiplodia theobromae	409:432	Lasiodiplodia theobromae	409:432	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	91	theme	concentrations	307:320	arg1	amount					251:256	A large amount	243:256	A large amount of GRAS (generally recognized as safe) salts and concentrations	243:320	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	91	theme	concentrations	307:320	arg1	concentrations					307:320	concentrations	307:320	concentrations	307:320	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	91	theme	concentrations	307:320	arg1	salts					297:301	GRAS (generally recognized as safe) salts	261:301	GRAS (generally recognized as safe) salts	261:301	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	4	92	dep	50	1141:1142	arg1	to					1138:1139	to	1138:1139	to	1138:1139	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	2	93	dep	carbonate	494:502	arg1	AC					505:506	AC	505:506	AC	505:506	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	93	dep	carbonate	494:502	arg1	%					512:512	0.2%	509:512	0.2%	509:512	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	3	94	theme	control	753:759	arg1	ability					761:767	Disease control ability	745:767	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts	745:905	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	4	95	theme	%	1059:1059	arg1	PS					1061:1062	2% PS	1058:1062	2% PS	1058:1062	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	6	96	theme	coated	1704:1709	arg1	fruit					1711:1715	coated fruit	1704:1715	coated fruit	1704:1715	Although the internal gas concentration (CO2 level) of coated fruit increased, the coatings did not induce off-flavors.					
31055161	4	97	theme	fruit	1273:1277	arg1	quality					1279:1285	postharvest fruit quality	1261:1285	postharvest fruit quality	1261:1285	Coatings containing 2% PS, 0.1% SEP, or 2% SB were the most effective reducing disease severity (up to 50% reduction) and were also applied to non-inoculated and cold-stored 'Barnfield' oranges to determine their effect on postharvest fruit quality.					
31055161	1	98	theme	large	245:249	arg1	amount					251:256	A large amount	243:256	A large amount of GRAS (generally recognized as safe) salts and concentrations	243:320	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	98	theme	large	245:249	arg1	concentrations					307:320	concentrations	307:320	concentrations	307:320	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	1	98	theme	large	245:249	arg1	salts					297:301	GRAS (generally recognized as safe) salts	261:301	GRAS (generally recognized as safe) salts	261:301	A large amount of GRAS (generally recognized as safe) salts and concentrations were evaluated in in vitro tests (inhibition of mycelial growth on PDA dishes) against Lasiodiplodia theobromae, the causal agent of citrus Diplodia stem-end rot.					
31055161	3	99	theme	Ortanique	949:957	arg1	mandarins					960:968	'Ortanique' mandarins	948:968	'Ortanique' mandarins	948:968	Disease control ability of edible composite coatings formulated with hydroxypropyl methylcellulose (HPMC), beeswax (BW), and these selected antifungal GRAS salts was assessed in in vivo experiments with 'Ortanique' mandarins and 'Barnfield' oranges artificially inoculated with L. theobromae.					
31055161	2	100	dep	silicate	687:694	arg1	PSi					697:699	PSi	697:699	PSi	697:699	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	100	dep	silicate	687:694	arg1	%					705:705	2.0%	702:705	2.0%	702:705	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	2	101	theme	potassium	677:685	arg1	silicate					687:694	potassium silicate	677:694	potassium silicate (PSi, 2.0%)	677:706	Ammonium carbonate (AC, 0.2%), potassium sorbate (PS, 2.0%), potassium carbonate (PC, 0.2%), sodium methylparaben (SMP, 0.1%), sodium ethylparaben (SEP, 0.1%), sodium benzoate (SB, 2.0%), and potassium silicate (PSi, 2.0%) were selected as the most effective.					
31055161	5	102	theme	uncoated	1625:1632	arg1	fruit					1642:1646	uncoated control fruit	1625:1646	uncoated control fruit	1625:1646	After periods of 21 and 42 d at 5 °C followed by 7 d of shelf life at 20 °C, coatings containing SEP and SB significantly reduced weight loss and did not adversely affect the physicochemical quality attributes (firmness, soluble solid content, titratable acidity, and ethanol and acetaldehyde content) and sensory flavor with respect to uncoated control fruit.					
31401273	0	0	theme	Kinetic	75:81	arg1	studies					112:118	Kinetic, thermodynamic and mechanism studies	75:118	Kinetic, thermodynamic and mechanism studies	75:118	Alginate-whey an effective and green adsorbent for crystal violet removal: Kinetic, thermodynamic and mechanism studies.					
31401273	8	1	theme	Calculated	1072:1081	arg1	energy					1127:1132	Gibbs free energy	1116:1132	Gibbs free energy (ΔG°)	1116:1138	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	8	1	theme	Calculated	1072:1081	arg1	parameters					1097:1106	Calculated thermodynamic parameters	1072:1106	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°)	1072:1173	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	8	1	theme	Calculated	1072:1081	arg1	enthalpy					1141:1148	enthalpy	1141:1148	enthalpy (ΔH°)	1141:1154	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	8	1	theme	Calculated	1072:1081	arg1	entropy					1161:1167	entropy	1161:1167	entropy (ΔS°)	1161:1173	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	9	2	theme	CV	1276:1277	arg1	molecules					1283:1291	CV dye molecules	1276:1291	CV dye molecules	1276:1291	Interactions of CV dye molecules with ALG-Aw composite beads were examined by FTIR and UV-visible DR spectroscopy.					
31401273	6	3	theme	removal	836:842	arg1	kinetic					822:828	kinetic	822:828	kinetic	822:828	The kinetic of CV removal onto ALG-Aw adsorbent can be described well by the pseudo-second order equation.					
31401273	1	4	theme	novel	138:142	arg1	biocomposite					144:155	a novel biocomposite beads	136:161	a novel biocomposite beads	136:161	In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw).					
31401273	1	5	theme	acid	222:225	arg1	Aw					233:234	Aw	233:234	Aw	233:234	In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw).					
31401273	1	5	theme	acid	222:225	arg1	whey					227:230	acid whey	222:230	acid whey (Aw)	222:235	In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw).					
31401273	0	6	theme	thermodynamic	84:96	arg1	studies					112:118	Kinetic, thermodynamic and mechanism studies	75:118	Kinetic, thermodynamic and mechanism studies	75:118	Alginate-whey an effective and green adsorbent for crystal violet removal: Kinetic, thermodynamic and mechanism studies.					
31401273	6	7	theme	CV	833:834	arg1	removal					836:842	CV removal	833:842	CV removal	833:842	The kinetic of CV removal onto ALG-Aw adsorbent can be described well by the pseudo-second order equation.					
31401273	9	8	with	Interactions	1260:1271	arg1	beads					1315:1319	ALG-Aw composite beads	1298:1319	ALG-Aw composite beads	1298:1319	Interactions of CV dye molecules with ALG-Aw composite beads were examined by FTIR and UV-visible DR spectroscopy.					
31401273	9	9	theme	composite	1305:1313	arg1	beads					1315:1319	ALG-Aw composite beads	1298:1319	ALG-Aw composite beads	1298:1319	Interactions of CV dye molecules with ALG-Aw composite beads were examined by FTIR and UV-visible DR spectroscopy.					
31401273	8	10	theme	thermodynamic	1083:1095	arg1	energy					1127:1132	Gibbs free energy	1116:1132	Gibbs free energy (ΔG°)	1116:1138	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	8	10	theme	thermodynamic	1083:1095	arg1	parameters					1097:1106	Calculated thermodynamic parameters	1072:1106	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°)	1072:1173	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	8	10	theme	thermodynamic	1083:1095	arg1	enthalpy					1141:1148	enthalpy	1141:1148	enthalpy (ΔH°)	1141:1154	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	8	10	theme	thermodynamic	1083:1095	arg1	entropy					1161:1167	entropy	1161:1167	entropy (ΔS°)	1161:1173	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	4	11	theme	adsorption	694:703	arg1	technique					705:713	a batch adsorption technique	686:713	a batch adsorption technique	686:713	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	6	12	theme	order	909:913	arg1	equation					915:922	the pseudo-second order equation	891:922	the pseudo-second order equation	891:922	The kinetic of CV removal onto ALG-Aw adsorbent can be described well by the pseudo-second order equation.					
31401273	0	13	theme	mechanism	102:110	arg1	studies					112:118	Kinetic, thermodynamic and mechanism studies	75:118	Kinetic, thermodynamic and mechanism studies	75:118	Alginate-whey an effective and green adsorbent for crystal violet removal: Kinetic, thermodynamic and mechanism studies.					
31401273	9	14	theme	UV-visible	1347:1356	arg1	spectroscopy					1361:1372	UV-visible DR spectroscopy	1347:1372	UV-visible DR spectroscopy	1347:1372	Interactions of CV dye molecules with ALG-Aw composite beads were examined by FTIR and UV-visible DR spectroscopy.					
31401273	3	15	theme	bacteria	473:480	arg1	presence					454:461	the presence	450:461	the presence of lactic bacteria immobilized in the alginate matrix leading to the formation of new porous structure	450:564	SEM analysis showed the presence of lactic bacteria immobilized in the alginate matrix leading to the formation of new porous structure.					
31401273	5	16	theme	adsorbent	791:799	arg1	dose					801:804	an adsorbent dose	788:804	an adsorbent dose of 0.4 g/L	788:815	The results showed that the maximum adsorption occurred at pH 6 and for an adsorbent dose of 0.4 g/L.					
31401273	7	17	theme	calculated	1038:1047	arg1	χ2					1053:1054	χ2	1053:1054	χ2	1053:1054	The equilibrium adsorption data of ALG-Aw followed well the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values.					
31401273	7	17	theme	calculated	1038:1047	arg1	R2					1049:1050	the calculated R2	1034:1050	the calculated R2	1034:1050	The equilibrium adsorption data of ALG-Aw followed well the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values.					
31401273	8	18	theme	Gibbs	1116:1120	arg1	energy					1127:1132	Gibbs free energy	1116:1132	Gibbs free energy (ΔG°)	1116:1138	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	8	18	theme	Gibbs	1116:1120	arg1	ΔG°					1135:1137	ΔG°	1135:1137	ΔG°	1135:1137	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	2	19	theme	corresponding	332:344	arg1	spectrum					346:353	the corresponding spectrum	328:353	the corresponding spectrum	328:353	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	5	20	theme	maximum	744:750	arg1	adsorption					752:761	the maximum adsorption	740:761	the maximum adsorption	740:761	The results showed that the maximum adsorption occurred at pH 6 and for an adsorbent dose of 0.4 g/L.					
31401273	3	21	theme	lactic	466:471	arg1	bacteria					473:480	lactic bacteria	466:480	lactic bacteria immobilized in the alginate matrix leading to the formation of new porous structure	466:564	SEM analysis showed the presence of lactic bacteria immobilized in the alginate matrix leading to the formation of new porous structure.					
31401273	2	22	theme	biocomposite	269:280	arg1	formation					242:250	The formation	238:250	The formation of alginate-whey biocomposite (ALG-Aw)	238:289	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	4	23	theme	crystal	625:631	arg1	CV					645:646	CV	645:646	CV	645:646	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	4	23	theme	crystal	625:631	arg1	dye					640:642	crystal violet dye	625:642	crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique	625:713	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	2	24	theme	bands	404:408	arg1	presence					366:373	the presence	362:373	the presence of characteristic absorption bands of both ALG and Aw	362:427	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	6	25	theme	pseudo-second	895:907	arg1	equation					915:922	the pseudo-second order equation	891:922	the pseudo-second order equation	891:922	The kinetic of CV removal onto ALG-Aw adsorbent can be described well by the pseudo-second order equation.					
31401273	2	26	theme	alginate-whey	255:267	arg1	ALG-Aw					283:288	ALG-Aw	283:288	ALG-Aw	283:288	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	2	26	theme	alginate-whey	255:267	arg1	biocomposite					269:280	alginate-whey biocomposite	255:280	alginate-whey biocomposite (ALG-Aw)	255:289	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	5	27	theme	0.4 g/L	809:815	arg1	dose					801:804	an adsorbent dose	788:804	an adsorbent dose of 0.4 g/L	788:815	The results showed that the maximum adsorption occurred at pH 6 and for an adsorbent dose of 0.4 g/L.					
31401273	2	28	theme	absorption	393:402	arg1	bands					404:408	characteristic absorption bands	378:408	characteristic absorption bands of both ALG and Aw	378:427	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	4	29	from	solutions	670:678	arg1	removal					649:655	crystal violet dye (CV) removal	625:655	crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique	625:713	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	3	30	theme	alginate	501:508	arg1	matrix					510:515	the alginate matrix	497:515	the alginate matrix leading to the formation of new porous structure	497:564	SEM analysis showed the presence of lactic bacteria immobilized in the alginate matrix leading to the formation of new porous structure.					
31401273	2	31	theme	characteristic	378:391	arg1	bands					404:408	characteristic absorption bands	378:408	characteristic absorption bands of both ALG and Aw	378:427	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	9	32	theme	DR	1358:1359	arg1	spectroscopy					1361:1372	UV-visible DR spectroscopy	1347:1372	UV-visible DR spectroscopy	1347:1372	Interactions of CV dye molecules with ALG-Aw composite beads were examined by FTIR and UV-visible DR spectroscopy.					
31401273	0	33	dep	studies	112:118	arg1	effective					17:25	effective	17:25	effective	17:25	Alginate-whey an effective and green adsorbent for crystal violet removal: Kinetic, thermodynamic and mechanism studies.					
31401273	0	33	dep	studies	112:118	arg1	green					31:35	green	31:35	green	31:35	Alginate-whey an effective and green adsorbent for crystal violet removal: Kinetic, thermodynamic and mechanism studies.					
31401273	4	34	theme	adsorption	571:580	arg1	properties					582:591	The adsorption properties	567:591	The adsorption properties of adsorbents	567:605	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	2	35	theme	Aw	426:427	arg1	bands					404:408	characteristic absorption bands	378:408	characteristic absorption bands of both ALG and Aw	378:427	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	7	36	theme	Redlich-Peterson	985:1000	arg1	isotherm					1002:1009	the Redlich-Peterson isotherm	981:1009	the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values	981:1069	The equilibrium adsorption data of ALG-Aw followed well the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values.					
31401273	7	36	theme	Redlich-Peterson	985:1000	arg1	coherent					1020:1027	coherent	1020:1027	coherent	1020:1027	The equilibrium adsorption data of ALG-Aw followed well the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values.					
31401273	4	37	theme	adsorbents	596:605	arg1	properties					582:591	The adsorption properties	567:591	The adsorption properties of adsorbents	567:605	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	1	38	theme	sodium	187:192	arg1	ALG					204:206	ALG	204:206	ALG	204:206	In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw).					
31401273	1	38	theme	sodium	187:192	arg1	alginate					194:201	sodium alginate	187:201	sodium alginate (ALG) solutions in acid whey (Aw)	187:235	In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw).					
31401273	9	39	theme	ALG-Aw	1298:1303	arg1	beads					1315:1319	ALG-Aw composite beads	1298:1319	ALG-Aw composite beads	1298:1319	Interactions of CV dye molecules with ALG-Aw composite beads were examined by FTIR and UV-visible DR spectroscopy.					
31401273	3	40	theme	new	545:547	arg1	structure					556:564	new porous structure	545:564	new porous structure	545:564	SEM analysis showed the presence of lactic bacteria immobilized in the alginate matrix leading to the formation of new porous structure.					
31401273	1	41	theme	alginate	194:201	arg1	solutions					209:217	sodium alginate (ALG) solutions	187:217	sodium alginate (ALG) solutions in acid whey (Aw)	187:235	In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw).					
31401273	2	42	theme	ALG	418:420	arg1	bands					404:408	characteristic absorption bands	378:408	characteristic absorption bands of both ALG and Aw	378:427	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	0	43	theme	violet	59:64	arg1	removal					66:72	crystal violet removal	51:72	crystal violet removal	51:72	Alginate-whey an effective and green adsorbent for crystal violet removal: Kinetic, thermodynamic and mechanism studies.					
31401273	1	44	dep	biocomposite	144:155	arg1	beads					157:161	beads	157:161	a novel biocomposite beads	136:161	In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw).					
31401273	8	45	theme	low	1242:1244	arg1	temperatures					1246:1257	low temperatures	1242:1257	low temperatures	1242:1257	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	7	46	with	coherent	1020:1027	arg1	χ2					1053:1054	χ2	1053:1054	χ2	1053:1054	The equilibrium adsorption data of ALG-Aw followed well the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values.					
31401273	7	46	with	coherent	1020:1027	arg1	R2					1049:1050	the calculated R2	1034:1050	the calculated R2	1034:1050	The equilibrium adsorption data of ALG-Aw followed well the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values.					
31401273	1	47	from	solutions	209:217	arg1	Aw					233:234	Aw	233:234	Aw	233:234	In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw).					
31401273	1	47	from	solutions	209:217	arg1	whey					227:230	acid whey	222:230	acid whey (Aw)	222:235	In this study, a novel biocomposite beads is developed by gelling sodium alginate (ALG) solutions in acid whey (Aw).					
31401273	7	48	theme	equilibrium	929:939	arg1	data					952:955	The equilibrium adsorption data	925:955	The equilibrium adsorption data of ALG-Aw	925:965	The equilibrium adsorption data of ALG-Aw followed well the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values.					
31401273	8	49	theme	free	1122:1125	arg1	energy					1127:1132	Gibbs free energy	1116:1132	Gibbs free energy (ΔG°)	1116:1138	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	8	49	theme	free	1122:1125	arg1	ΔG°					1135:1137	ΔG°	1135:1137	ΔG°	1135:1137	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	7	50	theme	ALG-Aw	960:965	arg1	data					952:955	The equilibrium adsorption data	925:955	The equilibrium adsorption data of ALG-Aw	925:965	The equilibrium adsorption data of ALG-Aw followed well the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values.					
31401273	4	51	theme	violet	633:638	arg1	CV					645:646	CV	645:646	CV	645:646	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	4	51	theme	violet	633:638	arg1	dye					640:642	crystal violet dye	625:642	crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique	625:713	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	4	52	theme	aqueous	662:668	arg1	solutions					670:678	aqueous solutions	662:678	aqueous solutions using a batch adsorption technique	662:713	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	4	53	theme	batch	688:692	arg1	technique					705:713	a batch adsorption technique	686:713	a batch adsorption technique	686:713	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	7	54	theme	adsorption	941:950	arg1	data					952:955	The equilibrium adsorption data	925:955	The equilibrium adsorption data of ALG-Aw	925:965	The equilibrium adsorption data of ALG-Aw followed well the Redlich-Peterson isotherm which is coherent with the calculated R2, χ2 and ARE values.					
31401273	0	55	dep	effective	17:25	arg1	adsorbent					37:45	adsorbent	37:45	adsorbent	37:45	Alginate-whey an effective and green adsorbent for crystal violet removal: Kinetic, thermodynamic and mechanism studies.					
31401273	3	56	theme	SEM	430:432	arg1	analysis					434:441	SEM analysis	430:441	SEM analysis	430:441	SEM analysis showed the presence of lactic bacteria immobilized in the alginate matrix leading to the formation of new porous structure.					
31401273	8	57	theme	adsorption	1186:1195	arg1	reaction					1197:1204	adsorption reaction	1186:1204	adsorption reaction	1186:1204	Calculated thermodynamic parameters such as Gibbs free energy (ΔG°), enthalpy (ΔH°), and entropy (ΔS°) shown that adsorption reaction is spontaneous and it is favored at low temperatures.					
31401273	3	58	theme	porous	549:554	arg1	structure					556:564	new porous structure	545:564	new porous structure	545:564	SEM analysis showed the presence of lactic bacteria immobilized in the alginate matrix leading to the formation of new porous structure.					
31401273	4	59	theme	dye	640:642	arg1	removal					649:655	crystal violet dye (CV) removal	625:655	crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique	625:713	The adsorption properties of adsorbents were evaluated in crystal violet dye (CV) removal from aqueous solutions using a batch adsorption technique.					
31401273	9	60	theme	dye	1279:1281	arg1	molecules					1283:1291	CV dye molecules	1276:1291	CV dye molecules	1276:1291	Interactions of CV dye molecules with ALG-Aw composite beads were examined by FTIR and UV-visible DR spectroscopy.					
31401273	0	61	theme	crystal	51:57	arg1	removal					66:72	crystal violet removal	51:72	crystal violet removal	51:72	Alginate-whey an effective and green adsorbent for crystal violet removal: Kinetic, thermodynamic and mechanism studies.					
31401273	3	62	theme	structure	556:564	arg1	formation					532:540	the formation	528:540	the formation of new porous structure	528:564	SEM analysis showed the presence of lactic bacteria immobilized in the alginate matrix leading to the formation of new porous structure.					
31401273	2	63	theme	FTIR	308:311	arg1	analysis					313:320	FTIR analysis	308:320	FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw	308:427	The formation of alginate-whey biocomposite (ALG-Aw) was confirmed by FTIR analysis where the corresponding spectrum showed the presence of characteristic absorption bands of both ALG and Aw.					
31401273	9	64	theme	molecules	1283:1291	arg1	Interactions					1260:1271	Interactions	1260:1271	Interactions of CV dye molecules with ALG-Aw composite beads	1260:1319	Interactions of CV dye molecules with ALG-Aw composite beads were examined by FTIR and UV-visible DR spectroscopy.					
30593419	3	0	theme	Fecal	292:296	arg1	transplantation					309:323	Fecal microbiota transplantation	292:323	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal	292:382	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	0	theme	Fecal	292:296	arg1	tool					398:401	a valuable tool	387:401	a valuable tool for targeted modification of microbiome leading to therapeutic response	387:473	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	0	theme	Fecal	292:296	arg1	FMT					326:328	FMT	326:328	FMT	326:328	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	0	1	from	transfer	18:25	arg1	disease					85:91	inflammatory bowel disease	66:91	inflammatory bowel disease	66:91	Reverse phenotype transfer via fecal microbial transplantation in inflammatory bowel disease.					
30593419	10	2	theme	broad	1358:1362	arg1	implications					1364:1375	broad implications	1358:1375	broad implications	1358:1375	Such reverse phenotype transfer has broad implications, especially in terms of preventive medicine.					
30593419	7	3	theme	transfer	924:931	arg1	hypothesis					882:891	the hypothesis	878:891	the hypothesis of so called reverse phenotype transfer in IBD	878:938	Based on our preliminary results, we propose the hypothesis of so called reverse phenotype transfer in IBD.					
30593419	11	4	theme	detailed	1431:1438	arg1	mechanisms					1440:1449	detailed mechanisms	1431:1449	detailed mechanisms	1431:1449	However, detailed mechanisms need to be elucidated to conclude the validity of the phenomenon.					
30593419	4	5	theme	gastrointestinal	533:548	arg1	diseases					582:589	gastrointestinal as well as non-gastrointestinal diseases	533:589	gastrointestinal as well as non-gastrointestinal diseases	533:589	Positive effect has been shown in therapy of a number of gastrointestinal as well as non-gastrointestinal diseases.					
30593419	3	6	theme	microbiota	298:307	arg1	transplantation					309:323	Fecal microbiota transplantation	292:323	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal	292:382	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	6	theme	microbiota	298:307	arg1	tool					398:401	a valuable tool	387:401	a valuable tool for targeted modification of microbiome leading to therapeutic response	387:473	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	6	theme	microbiota	298:307	arg1	FMT					326:328	FMT	326:328	FMT	326:328	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	1	7	from	disbalance	149:158	arg1	composition					167:177	the composition	163:177	the composition of intestinal microbiota	163:202	Inflammatory bowel disease (IBD) is characterized by a disbalance in the composition of intestinal microbiota.					
30593419	5	8	used	used	631:634	arg2	FMT					605:607	FMT	605:607	FMT	605:607	In addition, FMT has been successfully used to transfer the diseased phenotype form a donor with the disease to a healthy recipient.					
30593419	4	9	theme	Positive	476:483	arg1	effect					485:490	Positive effect	476:490	Positive effect	476:490	Positive effect has been shown in therapy of a number of gastrointestinal as well as non-gastrointestinal diseases.					
30593419	6	10	theme	microbiome	763:772	arg1	modification					743:754	targeted modification	734:754	targeted modification of the microbiome before the onset of colitis	734:800	However, targeted modification of the microbiome before the onset of colitis has not been shown previously.					
30593419	9	11	theme	improved	1208:1215	arg1	score					1225:1229	improved colitis score	1208:1229	improved colitis score compared with mice that received FMT from donors more resistant to development of colitis	1208:1319	Mice that received FMT from donors with severe colitis have shown improved colitis score compared with mice that received FMT from donors more resistant to development of colitis.					
30593419	10	12	theme	phenotype	1335:1343	arg1	transfer					1345:1352	Such reverse phenotype transfer	1322:1352	Such reverse phenotype transfer	1322:1352	Such reverse phenotype transfer has broad implications, especially in terms of preventive medicine.					
30593419	10	13	theme	preventive	1401:1410	arg1	medicine					1412:1419	preventive medicine	1401:1419	preventive medicine	1401:1419	Such reverse phenotype transfer has broad implications, especially in terms of preventive medicine.					
30593419	9	14	with	donors	1170:1175	arg1	colitis					1189:1195	severe colitis	1182:1195	severe colitis	1182:1195	Mice that received FMT from donors with severe colitis have shown improved colitis score compared with mice that received FMT from donors more resistant to development of colitis.					
30593419	8	15	from	donor	1031:1035	arg1	transplantation					990:1004	the transplantation	986:1004	the transplantation of gut microbiota from a donor more sensitive to IBD to a healthy recipient	986:1080	This term describes the phenomenon, in which the transplantation of gut microbiota from a donor more sensitive to IBD to a healthy recipient leads to resistance of the recipient to IBD and vice versa.					
30593419	3	16	from	donor	346:350	arg1	transplantation					309:323	Fecal microbiota transplantation	292:323	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal	292:382	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	16	from	donor	346:350	arg1	tool					398:401	a valuable tool	387:401	a valuable tool for targeted modification of microbiome leading to therapeutic response	387:473	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	16	from	donor	346:350	arg1	FMT					326:328	FMT	326:328	FMT	326:328	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	17	theme	valuable	389:396	arg1	transplantation					309:323	Fecal microbiota transplantation	292:323	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal	292:382	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	17	theme	valuable	389:396	arg1	tool					398:401	a valuable tool	387:401	a valuable tool for targeted modification of microbiome leading to therapeutic response	387:473	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	18	theme	diseased	368:375	arg1	animal					377:382	a patient or diseased animal	355:382	a patient or diseased animal	355:382	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	10	19	theme	reverse	1327:1333	arg1	transfer					1345:1352	Such reverse phenotype transfer	1322:1352	Such reverse phenotype transfer	1322:1352	Such reverse phenotype transfer has broad implications, especially in terms of preventive medicine.					
30593419	0	20	theme	phenotype	8:16	arg1	transfer					18:25	Reverse phenotype transfer	0:25	Reverse phenotype transfer via fecal microbial transplantation in inflammatory bowel disease	0:91	Reverse phenotype transfer via fecal microbial transplantation in inflammatory bowel disease.					
30593419	6	21	theme	targeted	734:741	arg1	modification					743:754	targeted modification	734:754	targeted modification of the microbiome before the onset of colitis	734:800	However, targeted modification of the microbiome before the onset of colitis has not been shown previously.					
30593419	1	22	theme	Inflammatory	94:105	arg1	IBD					122:124	IBD	122:124	IBD	122:124	Inflammatory bowel disease (IBD) is characterized by a disbalance in the composition of intestinal microbiota.					
30593419	1	22	theme	Inflammatory	94:105	arg1	disease					113:119	Inflammatory bowel disease	94:119	Inflammatory bowel disease (IBD)	94:125	Inflammatory bowel disease (IBD) is characterized by a disbalance in the composition of intestinal microbiota.					
30593419	9	23	theme	colitis	1217:1223	arg1	score					1225:1229	improved colitis score	1208:1229	improved colitis score compared with mice that received FMT from donors more resistant to development of colitis	1208:1319	Mice that received FMT from donors with severe colitis have shown improved colitis score compared with mice that received FMT from donors more resistant to development of colitis.					
30593419	10	24	contain	has	1354:1356	arg1	transfer					1345:1352	Such reverse phenotype transfer	1322:1352	Such reverse phenotype transfer	1322:1352	Such reverse phenotype transfer has broad implications, especially in terms of preventive medicine.					
30593419	10	24	contain	has	1354:1356	arg2	implications					1364:1375	broad implications	1358:1375	broad implications	1358:1375	Such reverse phenotype transfer has broad implications, especially in terms of preventive medicine.					
30593419	0	25	theme	Reverse	0:6	arg1	transfer					18:25	Reverse phenotype transfer	0:25	Reverse phenotype transfer via fecal microbial transplantation in inflammatory bowel disease	0:91	Reverse phenotype transfer via fecal microbial transplantation in inflammatory bowel disease.					
30593419	1	26	theme	bowel	107:111	arg1	IBD					122:124	IBD	122:124	IBD	122:124	Inflammatory bowel disease (IBD) is characterized by a disbalance in the composition of intestinal microbiota.					
30593419	1	26	theme	bowel	107:111	arg1	disease					113:119	Inflammatory bowel disease	94:119	Inflammatory bowel disease (IBD)	94:125	Inflammatory bowel disease (IBD) is characterized by a disbalance in the composition of intestinal microbiota.					
30593419	8	27	theme	healthy	1064:1070	arg1	recipient					1072:1080	a healthy recipient	1062:1080	a healthy recipient	1062:1080	This term describes the phenomenon, in which the transplantation of gut microbiota from a donor more sensitive to IBD to a healthy recipient leads to resistance of the recipient to IBD and vice versa.					
30593419	1	28	theme	intestinal	182:191	arg1	microbiota					193:202	intestinal microbiota	182:202	intestinal microbiota	182:202	Inflammatory bowel disease (IBD) is characterized by a disbalance in the composition of intestinal microbiota.					
30593419	3	29	theme	healthy	338:344	arg1	donor					346:350	a healthy donor	336:350	a healthy donor	336:350	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	8	30	theme	microbiota	1013:1022	arg1	transplantation					990:1004	the transplantation	986:1004	the transplantation of gut microbiota from a donor more sensitive to IBD to a healthy recipient	986:1080	This term describes the phenomenon, in which the transplantation of gut microbiota from a donor more sensitive to IBD to a healthy recipient leads to resistance of the recipient to IBD and vice versa.					
30593419	2	31	theme	disease	277:283	arg1	state					285:289	a disease state	275:289	a disease state	275:289	It is not clear whether such dysbiosis is a cause or a consequence of a disease state.					
30593419	7	32	theme	called	899:904	arg1	transfer					924:931	so called reverse phenotype transfer	896:931	so called reverse phenotype transfer	896:931	Based on our preliminary results, we propose the hypothesis of so called reverse phenotype transfer in IBD.					
30593419	1	33	theme	microbiota	193:202	arg1	composition					167:177	the composition	163:177	the composition of intestinal microbiota	163:202	Inflammatory bowel disease (IBD) is characterized by a disbalance in the composition of intestinal microbiota.					
30593419	2	34	theme	state	285:289	arg1	consequence					260:270	a consequence	258:270	a consequence of a disease state	258:289	It is not clear whether such dysbiosis is a cause or a consequence of a disease state.					
30593419	2	34	theme	state	285:289	arg1	dysbiosis					234:242	such dysbiosis	229:242	such dysbiosis	229:242	It is not clear whether such dysbiosis is a cause or a consequence of a disease state.					
30593419	2	34	theme	state	285:289	arg1	cause					249:253	a cause	247:253	a cause	247:253	It is not clear whether such dysbiosis is a cause or a consequence of a disease state.					
30593419	4	35	theme	number	523:528	arg1	therapy					510:516	therapy	510:516	therapy of a number of gastrointestinal as well as non-gastrointestinal diseases	510:589	Positive effect has been shown in therapy of a number of gastrointestinal as well as non-gastrointestinal diseases.					
30593419	8	36	theme	gut	1009:1011	arg1	microbiota					1013:1022	gut microbiota	1009:1022	gut microbiota	1009:1022	This term describes the phenomenon, in which the transplantation of gut microbiota from a donor more sensitive to IBD to a healthy recipient leads to resistance of the recipient to IBD and vice versa.					
30593419	5	37	with	donor	678:682	arg1	disease					693:699	the disease	689:699	the disease	689:699	In addition, FMT has been successfully used to transfer the diseased phenotype form a donor with the disease to a healthy recipient.					
30593419	0	38	theme	microbial	37:45	arg1	transplantation					47:61	fecal microbial transplantation	31:61	fecal microbial transplantation	31:61	Reverse phenotype transfer via fecal microbial transplantation in inflammatory bowel disease.					
30593419	2	39	theme	such	229:232	arg1	dysbiosis					234:242	such dysbiosis	229:242	such dysbiosis	229:242	It is not clear whether such dysbiosis is a cause or a consequence of a disease state.					
30593419	2	39	theme	such	229:232	arg1	cause					249:253	a cause	247:253	a cause	247:253	It is not clear whether such dysbiosis is a cause or a consequence of a disease state.					
30593419	0	40	theme	fecal	31:35	arg1	transplantation					47:61	fecal microbial transplantation	31:61	fecal microbial transplantation	31:61	Reverse phenotype transfer via fecal microbial transplantation in inflammatory bowel disease.					
30593419	9	41	theme	colitis	1313:1319	arg1	development					1298:1308	development	1298:1308	development of colitis	1298:1319	Mice that received FMT from donors with severe colitis have shown improved colitis score compared with mice that received FMT from donors more resistant to development of colitis.					
30593419	3	42	theme	targeted	407:414	arg1	modification					416:427	targeted modification	407:427	targeted modification of microbiome leading to therapeutic response	407:473	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	3	43	theme	patient	357:363	arg1	animal					377:382	a patient or diseased animal	355:382	a patient or diseased animal	355:382	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	5	44	theme	healthy	706:712	arg1	recipient					714:722	a healthy recipient	704:722	a healthy recipient	704:722	In addition, FMT has been successfully used to transfer the diseased phenotype form a donor with the disease to a healthy recipient.					
30593419	10	45	theme	Such	1322:1325	arg1	transfer					1345:1352	Such reverse phenotype transfer	1322:1352	Such reverse phenotype transfer	1322:1352	Such reverse phenotype transfer has broad implications, especially in terms of preventive medicine.					
30593419	11	46	theme	phenomenon	1505:1514	arg1	validity					1489:1496	the validity	1485:1496	the validity of the phenomenon	1485:1514	However, detailed mechanisms need to be elucidated to conclude the validity of the phenomenon.					
30593419	10	47	theme	medicine	1412:1419	arg1	terms					1392:1396	terms	1392:1396	terms of preventive medicine	1392:1419	Such reverse phenotype transfer has broad implications, especially in terms of preventive medicine.					
30593419	6	48	theme	colitis	794:800	arg1	onset					785:789	the onset	781:789	the onset of colitis	781:800	However, targeted modification of the microbiome before the onset of colitis has not been shown previously.					
30593419	3	49	theme	therapeutic	454:464	arg1	response					466:473	therapeutic response	454:473	therapeutic response	454:473	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	7	50	theme	reverse	906:912	arg1	transfer					924:931	so called reverse phenotype transfer	896:931	so called reverse phenotype transfer	896:931	Based on our preliminary results, we propose the hypothesis of so called reverse phenotype transfer in IBD.					
30593419	3	51	theme	microbiome	432:441	arg1	modification					416:427	targeted modification	407:427	targeted modification of microbiome leading to therapeutic response	407:473	Fecal microbiota transplantation (FMT) from a healthy donor to a patient or diseased animal is a valuable tool for targeted modification of microbiome leading to therapeutic response.					
30593419	4	52	theme	non-gastrointestinal	561:580	arg1	diseases					582:589	gastrointestinal as well as non-gastrointestinal diseases	533:589	gastrointestinal as well as non-gastrointestinal diseases	533:589	Positive effect has been shown in therapy of a number of gastrointestinal as well as non-gastrointestinal diseases.					
30593419	5	53	theme	diseased	652:659	arg1	phenotype					661:669	the diseased phenotype	648:669	the diseased phenotype	648:669	In addition, FMT has been successfully used to transfer the diseased phenotype form a donor with the disease to a healthy recipient.					
30593419	7	54	from	hypothesis	882:891	arg1	IBD					936:938	IBD	936:938	IBD	936:938	Based on our preliminary results, we propose the hypothesis of so called reverse phenotype transfer in IBD.					
30593419	0	55	theme	bowel	79:83	arg1	disease					85:91	inflammatory bowel disease	66:91	inflammatory bowel disease	66:91	Reverse phenotype transfer via fecal microbial transplantation in inflammatory bowel disease.					
30593419	7	56	theme	preliminary	846:856	arg1	results					858:864	our preliminary results	842:864	our preliminary results	842:864	Based on our preliminary results, we propose the hypothesis of so called reverse phenotype transfer in IBD.					
30593419	0	57	theme	inflammatory	66:77	arg1	disease					85:91	inflammatory bowel disease	66:91	inflammatory bowel disease	66:91	Reverse phenotype transfer via fecal microbial transplantation in inflammatory bowel disease.					
30593419	9	58	theme	severe	1182:1187	arg1	colitis					1189:1195	severe colitis	1182:1195	severe colitis	1182:1195	Mice that received FMT from donors with severe colitis have shown improved colitis score compared with mice that received FMT from donors more resistant to development of colitis.					
30593419	8	59	theme	recipient	1109:1117	arg1	resistance					1091:1100	resistance	1091:1100	resistance of the recipient to IBD	1091:1124	This term describes the phenomenon, in which the transplantation of gut microbiota from a donor more sensitive to IBD to a healthy recipient leads to resistance of the recipient to IBD and vice versa.					
30593419	7	60	theme	phenotype	914:922	arg1	transfer					924:931	so called reverse phenotype transfer	896:931	so called reverse phenotype transfer	896:931	Based on our preliminary results, we propose the hypothesis of so called reverse phenotype transfer in IBD.					
30593419	9	61	theme	resistant	1285:1293	arg1	donors					1273:1278	donors	1273:1278	donors more resistant to development of colitis	1273:1319	Mice that received FMT from donors with severe colitis have shown improved colitis score compared with mice that received FMT from donors more resistant to development of colitis.					
30593419	8	62	theme	sensitive	1042:1050	arg1	donor					1031:1035	a donor	1029:1035	a donor more sensitive to IBD to a healthy recipient	1029:1080	This term describes the phenomenon, in which the transplantation of gut microbiota from a donor more sensitive to IBD to a healthy recipient leads to resistance of the recipient to IBD and vice versa.					
30593419	4	63	theme	diseases	582:589	arg1	number					523:528	a number	521:528	a number of gastrointestinal as well as non-gastrointestinal diseases	521:589	Positive effect has been shown in therapy of a number of gastrointestinal as well as non-gastrointestinal diseases.					
30131088	9	0	theme	LP	1356:1357	arg1	Heritabilities					1338:1351	Heritabilities	1338:1351	Heritabilities of LP and LY	1338:1364	Heritabilities of LP and LY were 0.43±0.03 and 0.14±0.02, respectively, and LP and protein percentage were the most repeatable traits.					
30131088	4	1	theme	bivariate	826:834	arg1	models					836:841	bivariate models	826:841	bivariate models	826:841	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	9	2	theme	LY	1363:1364	arg1	Heritabilities					1338:1351	Heritabilities	1338:1351	Heritabilities of LP and LY	1338:1364	Heritabilities of LP and LY were 0.43±0.03 and 0.14±0.02, respectively, and LP and protein percentage were the most repeatable traits.					
30131088	12	3	theme	breeding	1856:1863	arg1	strategies					1865:1874	breeding strategies to accelerate the gain of correlated low heritable traits	1856:1932	breeding strategies to accelerate the gain of correlated low heritable traits	1856:1932	Results suggest that milk LP could be considered in breeding strategies to accelerate the gain of correlated low heritable traits.					
30131088	4	4	theme	milk	785:788	arg1	point					799:803	milk freezing point	785:803	milk freezing point	785:803	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	13	5	theme	Italian	2032:2038	arg1	population					2049:2058	Italian Holstein population	2032:2058	Italian Holstein population	2032:2058	Further research is needed to evaluate the feasibility of including LP in the selection index of Italian Holstein population to address country-specific needs and market demands.					
30131088	11	6	with	relationships	1623:1635	arg1	percentage					1680:1689	fat percentage	1676:1689	fat percentage (r a=-0.71±0.06)	1676:1706	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	6	with	relationships	1623:1635	arg1	percentage					1717:1726	protein percentage	1709:1726	protein percentage (r a=-0.57±0.06)	1709:1743	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	6	with	relationships	1623:1635	arg1	r					1660:1660	r a=0.97±0.00	1660:1672	r a=0.97±0.00	1660:1672	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	6	with	relationships	1623:1635	arg1	yield					1757:1761	protein yield	1749:1761	protein yield (r a=0.64±0.06)	1749:1777	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	6	with	relationships	1623:1635	arg1	r					1729:1729	r a=-0.57±0.06	1729:1742	r a=-0.57±0.06	1729:1742	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	6	with	relationships	1623:1635	arg1	r					1692:1692	r a=-0.71±0.06	1692:1705	r a=-0.71±0.06	1692:1705	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	6	with	relationships	1623:1635	arg1	a=0.64±0.06					1766:1776	r a=0.64±0.06	1764:1776	r a=0.64±0.06	1764:1776	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	6	with	relationships	1623:1635	arg1	yield					1653:1657	milk yield	1648:1657	milk yield (r a=0.97±0.00)	1648:1673	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	12	7	theme	milk	1825:1828	arg1	LP					1830:1831	milk LP	1825:1831	milk LP	1825:1831	Results suggest that milk LP could be considered in breeding strategies to accelerate the gain of correlated low heritable traits.					
30131088	6	8	theme	permanent	1082:1090	arg1	environment					1092:1102	lactation permanent environment	1072:1102	lactation permanent environment	1072:1102	Random effects were animal additive genetic, within and across lactation permanent environment and the residual.					
30131088	4	9	theme	yield	584:588	arg1	Heritabilities					551:564	Heritabilities	551:564	Heritabilities of LP and lactose yield (LY)	551:593	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	5	10	theme	lactation	998:1006	arg1	stage					989:993	stage	989:993	stage	989:993	Fixed effects included in the analyses were herd-test-date, season of calving, parity, stage of lactation and the interaction between parity and stage of lactation.					
30131088	5	10	theme	lactation	998:1006	arg1	parity					978:983	parity	978:983	parity	978:983	Fixed effects included in the analyses were herd-test-date, season of calving, parity, stage of lactation and the interaction between parity and stage of lactation.					
30131088	12	11	theme	traits	1927:1932	arg1	gain					1894:1897	the gain	1890:1897	the gain of correlated low heritable traits	1890:1932	Results suggest that milk LP could be considered in breeding strategies to accelerate the gain of correlated low heritable traits.					
30131088	10	12	theme	cell	1519:1522	arg1	score					1524:1528	somatic cell score	1511:1528	somatic cell score	1511:1528	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	4	13	with	correlations	691:702	arg1	point					799:803	milk freezing point	785:803	milk freezing point	785:803	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	4	13	with	correlations	691:702	arg1	traits					754:759	production traits	743:759	production traits	743:759	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	4	13	with	correlations	691:702	arg1	composition					727:737	milk composition	722:737	milk composition	722:737	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	4	13	with	correlations	691:702	arg1	score					775:779	somatic cell score	762:779	somatic cell score	762:779	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	11	14	theme	milk	1648:1651	arg1	r					1660:1660	r a=0.97±0.00	1660:1672	r a=0.97±0.00	1660:1672	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	14	theme	milk	1648:1651	arg1	yield					1653:1657	milk yield	1648:1657	milk yield (r a=0.97±0.00)	1648:1673	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	4	15	theme	repeatability	631:643	arg1	models					652:657	single-trait repeatability animal models	618:657	single-trait repeatability animal models	618:657	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	3	16	from	cows	532:535	arg1	records					505:511	59 811 test-day records	489:511	59 811 test-day records from 4355 Holstein cows in 266 herds	489:548	Data used in the present paper consisted of 59 811 test-day records from 4355 Holstein cows in 266 herds.					
30131088	11	17	theme	LY	1640:1641	arg1	relationships					1623:1635	Genetic relationships	1615:1635	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06)	1615:1777	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	0	18	theme	relationships	55:67	arg1	freezing					122:129	freezing	122:129	freezing	122:129	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	0	18	theme	relationships	55:67	arg1	repeatability					17:29	repeatability	17:29	repeatability	17:29	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	0	18	theme	relationships	55:67	arg1	Heritability					0:11	Heritability	0:11	Heritability	0:11	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	0	18	theme	relationships	55:67	arg1	score					112:116	somatic cell score	99:116	somatic cell score	99:116	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	12	19	theme	low	1913:1915	arg1	traits					1927:1932	correlated low heritable traits	1902:1932	correlated low heritable traits	1902:1932	Results suggest that milk LP could be considered in breeding strategies to accelerate the gain of correlated low heritable traits.					
30131088	0	20	theme	traditional	74:84	arg1	traits					91:96	traditional milk traits	74:96	traditional milk traits	74:96	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	9	21	theme	repeatable	1454:1463	arg1	traits					1465:1470	the most repeatable traits	1445:1470	the most repeatable traits	1445:1470	Heritabilities of LP and LY were 0.43±0.03 and 0.14±0.02, respectively, and LP and protein percentage were the most repeatable traits.					
30131088	9	21	theme	repeatable	1454:1463	arg1	percentage					1429:1438	protein percentage	1421:1438	protein percentage	1421:1438	Heritabilities of LP and LY were 0.43±0.03 and 0.14±0.02, respectively, and LP and protein percentage were the most repeatable traits.					
30131088	9	21	theme	repeatable	1454:1463	arg1	LP					1414:1415	LP	1414:1415	LP	1414:1415	Heritabilities of LP and LY were 0.43±0.03 and 0.14±0.02, respectively, and LP and protein percentage were the most repeatable traits.					
30131088	4	22	theme	LY	714:715	arg1	correlations					691:702	genetic and phenotypic correlations	668:702	genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point	668:803	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	3	23	from	records	505:511	arg1	herds					544:548	266 herds	540:548	266 herds	540:548	Data used in the present paper consisted of 59 811 test-day records from 4355 Holstein cows in 266 herds.					
30131088	1	24	theme	official	312:319	arg1	procedures					336:345	the official milk recording procedures	308:345	the official milk recording procedures	308:345	Lactose percentage (LP) in milk is currently determined in most herd-testing schemes, and globally, it is usually routinely recorded in the framework of the official milk recording procedures.					
30131088	5	25	theme	calving	914:920	arg1	season					904:909	season	904:909	season of calving, parity, stage of lactation	904:948	Fixed effects included in the analyses were herd-test-date, season of calving, parity, stage of lactation and the interaction between parity and stage of lactation.					
30131088	5	25	theme	calving	914:920	arg1	interaction					958:968	the interaction	954:968	the interaction between parity and stage of lactation	954:1006	Fixed effects included in the analyses were herd-test-date, season of calving, parity, stage of lactation and the interaction between parity and stage of lactation.					
30131088	5	25	theme	calving	914:920	arg1	herd-test-date					888:901	herd-test-date	888:901	herd-test-date	888:901	Fixed effects included in the analyses were herd-test-date, season of calving, parity, stage of lactation and the interaction between parity and stage of lactation.					
30131088	4	26	theme	milk	722:725	arg1	composition					727:737	milk composition	722:737	milk composition	722:737	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	2	27	theme	few	357:359	arg1	studies					361:367	few studies	357:367	few studies	357:367	However, few studies have investigated the phenotypic and genetic variability of this component.					
30131088	0	28	theme	somatic	99:105	arg1	score					112:116	somatic cell score	99:116	somatic cell score	99:116	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	1	29	theme	recording	326:334	arg1	procedures					336:345	the official milk recording procedures	308:345	the official milk recording procedures	308:345	Lactose percentage (LP) in milk is currently determined in most herd-testing schemes, and globally, it is usually routinely recorded in the framework of the official milk recording procedures.					
30131088	6	30	theme	Random	1009:1014	arg1	effects					1016:1022	Random effects	1009:1022	Random effects	1009:1022	Random effects were animal additive genetic, within and across lactation permanent environment and the residual.					
30131088	10	31	theme	milk	1538:1541	arg1	point					1552:1556	milk freezing point	1538:1556	milk freezing point	1538:1556	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	7	32	theme	Lactation	1122:1130	arg1	curves					1132:1137	Lactation curves	1122:1137	Lactation curves of LP and LY	1122:1150	Lactation curves of LP and LY increased from parturition to the peak of lactation and decreased thereafter, mirroring the typical curve of milk yield.					
30131088	4	33	theme	LP	707:708	arg1	correlations					691:702	genetic and phenotypic correlations	668:702	genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point	668:803	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	7	34	theme	typical	1244:1250	arg1	curve					1252:1256	the typical curve	1240:1256	the typical curve of milk yield	1240:1270	Lactation curves of LP and LY increased from parturition to the peak of lactation and decreased thereafter, mirroring the typical curve of milk yield.					
30131088	4	35	theme	somatic	762:768	arg1	score					775:779	somatic cell score	762:779	somatic cell score	762:779	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	0	36	theme	milk	34:37	arg1	lactose					39:45	milk lactose	34:45	milk lactose	34:45	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	13	37	theme	Holstein	2040:2047	arg1	population					2049:2058	Italian Holstein population	2032:2058	Italian Holstein population	2032:2058	Further research is needed to evaluate the feasibility of including LP in the selection index of Italian Holstein population to address country-specific needs and market demands.					
30131088	11	38	theme	protein	1709:1715	arg1	r					1729:1729	r a=-0.57±0.06	1729:1742	r a=-0.57±0.06	1729:1742	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	38	theme	protein	1709:1715	arg1	percentage					1717:1726	protein percentage	1709:1726	protein percentage (r a=-0.57±0.06)	1709:1743	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	13	39	theme	selection	2013:2021	arg1	index					2023:2027	the selection index	2009:2027	the selection index of Italian Holstein population	2009:2058	Further research is needed to evaluate the feasibility of including LP in the selection index of Italian Holstein population to address country-specific needs and market demands.					
30131088	4	40	dep	estimated	600:608	arg1	whereas					660:666	whereas	660:666	whereas	660:666	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	11	41	theme	Genetic	1615:1621	arg1	relationships					1623:1635	Genetic relationships	1615:1635	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06)	1615:1777	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	5	42	theme	lactation	940:948	arg1	calving					914:920	calving	914:920	calving	914:920	Fixed effects included in the analyses were herd-test-date, season of calving, parity, stage of lactation and the interaction between parity and stage of lactation.					
30131088	5	42	theme	lactation	940:948	arg1	stage					931:935	stage	931:935	stage of lactation	931:948	Fixed effects included in the analyses were herd-test-date, season of calving, parity, stage of lactation and the interaction between parity and stage of lactation.					
30131088	12	43	theme	correlated	1902:1911	arg1	traits					1927:1932	correlated low heritable traits	1902:1932	correlated low heritable traits	1902:1932	Results suggest that milk LP could be considered in breeding strategies to accelerate the gain of correlated low heritable traits.					
30131088	4	44	theme	LP	569:570	arg1	Heritabilities					551:564	Heritabilities	551:564	Heritabilities of LP and lactose yield (LY)	551:593	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	7	45	theme	LY	1149:1150	arg1	curves					1132:1137	Lactation curves	1122:1137	Lactation curves of LP and LY	1122:1150	Lactation curves of LP and LY increased from parturition to the peak of lactation and decreased thereafter, mirroring the typical curve of milk yield.					
30131088	3	46	theme	present	462:468	arg1	paper					470:474	the present paper	458:474	the present paper	458:474	Data used in the present paper consisted of 59 811 test-day records from 4355 Holstein cows in 266 herds.					
30131088	1	47	theme	herd-testing	219:230	arg1	schemes					232:238	most herd-testing schemes	214:238	most herd-testing schemes	214:238	Lactose percentage (LP) in milk is currently determined in most herd-testing schemes, and globally, it is usually routinely recorded in the framework of the official milk recording procedures.					
30131088	3	48	theme	test-day	496:503	arg1	records					505:511	59 811 test-day records	489:511	59 811 test-day records from 4355 Holstein cows in 266 herds	489:548	Data used in the present paper consisted of 59 811 test-day records from 4355 Holstein cows in 266 herds.					
30131088	10	49	with	correlations	1481:1492	arg1	score					1524:1528	somatic cell score	1511:1528	somatic cell score	1511:1528	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	10	49	with	correlations	1481:1492	arg1	point					1552:1556	milk freezing point	1538:1556	milk freezing point	1538:1556	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	10	49	with	correlations	1481:1492	arg1	LY					1531:1532	LY	1531:1532	LY	1531:1532	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	10	50	theme	r	1495:1495	arg1	correlations					1481:1492	Genetic correlations	1473:1492	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point	1473:1556	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	10	50	theme	r	1495:1495	arg1	a					1497:1497	r a	1495:1497	r a	1495:1497	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	0	51	with	relationships	55:67	arg1	traits					91:96	traditional milk traits	74:96	traditional milk traits	74:96	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	1	52	theme	Lactose	155:161	arg1	LP					175:176	LP	175:176	LP	175:176	Lactose percentage (LP) in milk is currently determined in most herd-testing schemes, and globally, it is usually routinely recorded in the framework of the official milk recording procedures.					
30131088	1	52	theme	Lactose	155:161	arg1	percentage					163:172	Lactose percentage	155:172	Lactose percentage (LP) in milk	155:185	Lactose percentage (LP) in milk is currently determined in most herd-testing schemes, and globally, it is usually routinely recorded in the framework of the official milk recording procedures.					
30131088	10	53	theme	somatic	1511:1517	arg1	score					1524:1528	somatic cell score	1511:1528	somatic cell score	1511:1528	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	3	54	theme	Holstein	523:530	arg1	cows					532:535	4355 Holstein cows	518:535	4355 Holstein cows	518:535	Data used in the present paper consisted of 59 811 test-day records from 4355 Holstein cows in 266 herds.					
30131088	4	55	theme	freezing	790:797	arg1	point					799:803	milk freezing point	785:803	milk freezing point	785:803	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	10	56	theme	LP	1503:1504	arg1	correlations					1481:1492	Genetic correlations	1473:1492	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point	1473:1556	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	10	56	theme	LP	1503:1504	arg1	a					1497:1497	r a	1495:1497	r a	1495:1497	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	4	57	theme	cell	770:773	arg1	score					775:779	somatic cell score	762:779	somatic cell score	762:779	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	6	58	theme	lactation	1072:1080	arg1	environment					1092:1102	lactation permanent environment	1072:1102	lactation permanent environment	1072:1102	Random effects were animal additive genetic, within and across lactation permanent environment and the residual.					
30131088	4	59	theme	lactose	576:582	arg1	LY					591:592	LY	591:592	LY	591:592	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	4	59	theme	lactose	576:582	arg1	yield					584:588	lactose yield	576:588	lactose yield (LY)	576:593	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	11	60	theme	fat	1676:1678	arg1	percentage					1680:1689	fat percentage	1676:1689	fat percentage (r a=-0.71±0.06)	1676:1706	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	60	theme	fat	1676:1678	arg1	r					1692:1692	r a=-0.71±0.06	1692:1705	r a=-0.71±0.06	1692:1705	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	0	61	theme	Holstein	140:147	arg1	cows					149:152	Holstein cows	140:152	Holstein cows	140:152	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	12	62	theme	heritable	1917:1925	arg1	traits					1927:1932	correlated low heritable traits	1902:1932	correlated low heritable traits	1902:1932	Results suggest that milk LP could be considered in breeding strategies to accelerate the gain of correlated low heritable traits.					
30131088	9	63	theme	protein	1421:1427	arg1	traits					1465:1470	the most repeatable traits	1445:1470	the most repeatable traits	1445:1470	Heritabilities of LP and LY were 0.43±0.03 and 0.14±0.02, respectively, and LP and protein percentage were the most repeatable traits.					
30131088	9	63	theme	protein	1421:1427	arg1	percentage					1429:1438	protein percentage	1421:1438	protein percentage	1421:1438	Heritabilities of LP and LY were 0.43±0.03 and 0.14±0.02, respectively, and LP and protein percentage were the most repeatable traits.					
30131088	9	63	theme	protein	1421:1427	arg1	LP					1414:1415	LP	1414:1415	LP	1414:1415	Heritabilities of LP and LY were 0.43±0.03 and 0.14±0.02, respectively, and LP and protein percentage were the most repeatable traits.					
30131088	8	64	theme	Lactose	1273:1279	arg1	percentage					1281:1290	Lactose percentage	1273:1290	Lactose percentage	1273:1290	Lactose percentage was greater in first- than later-parity cows.					
30131088	4	65	theme	genetic	668:674	arg1	correlations					691:702	genetic and phenotypic correlations	668:702	genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point	668:803	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	4	66	theme	single-trait	618:629	arg1	models					652:657	single-trait repeatability animal models	618:657	single-trait repeatability animal models	618:657	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	0	67	theme	milk	86:89	arg1	traits					91:96	traditional milk traits	74:96	traditional milk traits	74:96	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	11	68	theme	r	1764:1764	arg1	yield					1757:1761	protein yield	1749:1761	protein yield (r a=0.64±0.06)	1749:1777	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	68	theme	r	1764:1764	arg1	a=0.64±0.06					1766:1776	r a=0.64±0.06	1764:1776	r a=0.64±0.06	1764:1776	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	1	69	from	percentage	163:172	arg1	milk					182:185	milk	182:185	milk	182:185	Lactose percentage (LP) in milk is currently determined in most herd-testing schemes, and globally, it is usually routinely recorded in the framework of the official milk recording procedures.					
30131088	5	70	theme	Fixed	844:848	arg1	effects					850:856	Fixed effects	844:856	Fixed effects included in the analyses	844:881	Fixed effects included in the analyses were herd-test-date, season of calving, parity, stage of lactation and the interaction between parity and stage of lactation.					
30131088	4	71	theme	animal	645:650	arg1	models					652:657	single-trait repeatability animal models	618:657	single-trait repeatability animal models	618:657	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	7	72	theme	LP	1142:1143	arg1	curves					1132:1137	Lactation curves	1122:1137	Lactation curves of LP and LY	1122:1150	Lactation curves of LP and LY increased from parturition to the peak of lactation and decreased thereafter, mirroring the typical curve of milk yield.					
30131088	13	73	theme	Further	1935:1941	arg1	research					1943:1950	Further research	1935:1950	Further research	1935:1950	Further research is needed to evaluate the feasibility of including LP in the selection index of Italian Holstein population to address country-specific needs and market demands.					
30131088	0	74	theme	cell	107:110	arg1	score					112:116	somatic cell score	99:116	somatic cell score	99:116	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	1	75	theme	milk	321:324	arg1	procedures					336:345	the official milk recording procedures	308:345	the official milk recording procedures	308:345	Lactose percentage (LP) in milk is currently determined in most herd-testing schemes, and globally, it is usually routinely recorded in the framework of the official milk recording procedures.					
30131088	7	76	theme	milk	1261:1264	arg1	yield					1266:1270	milk yield	1261:1270	milk yield	1261:1270	Lactation curves of LP and LY increased from parturition to the peak of lactation and decreased thereafter, mirroring the typical curve of milk yield.					
30131088	10	77	theme	freezing	1543:1550	arg1	point					1552:1556	milk freezing point	1538:1556	milk freezing point	1538:1556	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	1	78	theme	procedures	336:345	arg1	framework					295:303	the framework	291:303	the framework of the official milk recording procedures	291:345	Lactose percentage (LP) in milk is currently determined in most herd-testing schemes, and globally, it is usually routinely recorded in the framework of the official milk recording procedures.					
30131088	2	79	theme	genetic	406:412	arg1	variability					414:424	the phenotypic and genetic variability	387:424	the phenotypic and genetic variability of this component	387:442	However, few studies have investigated the phenotypic and genetic variability of this component.					
30131088	4	80	theme	phenotypic	680:689	arg1	correlations					691:702	genetic and phenotypic correlations	668:702	genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point	668:803	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	2	81	theme	phenotypic	391:400	arg1	variability					414:424	the phenotypic and genetic variability	387:424	the phenotypic and genetic variability of this component	387:442	However, few studies have investigated the phenotypic and genetic variability of this component.					
30131088	0	82	theme	lactose	39:45	arg1	freezing					122:129	freezing	122:129	freezing	122:129	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	0	82	theme	lactose	39:45	arg1	repeatability					17:29	repeatability	17:29	repeatability	17:29	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	0	82	theme	lactose	39:45	arg1	Heritability					0:11	Heritability	0:11	Heritability	0:11	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	0	82	theme	lactose	39:45	arg1	score					112:116	somatic cell score	99:116	somatic cell score	99:116	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	13	83	theme	market	2098:2103	arg1	demands					2105:2111	market demands	2098:2111	market demands	2098:2111	Further research is needed to evaluate the feasibility of including LP in the selection index of Italian Holstein population to address country-specific needs and market demands.					
30131088	2	84	theme	component	434:442	arg1	variability					414:424	the phenotypic and genetic variability	387:424	the phenotypic and genetic variability of this component	387:442	However, few studies have investigated the phenotypic and genetic variability of this component.					
30131088	13	85	from	LP	2003:2004	arg1	index					2023:2027	the selection index	2009:2027	the selection index of Italian Holstein population	2009:2058	Further research is needed to evaluate the feasibility of including LP in the selection index of Italian Holstein population to address country-specific needs and market demands.					
30131088	8	86	theme	later-parity	1319:1330	arg1	cows					1332:1335	later-parity cows	1319:1335	later-parity cows	1319:1335	Lactose percentage was greater in first- than later-parity cows.					
30131088	13	87	theme	population	2049:2058	arg1	index					2023:2027	the selection index	2009:2027	the selection index of Italian Holstein population	2009:2058	Further research is needed to evaluate the feasibility of including LP in the selection index of Italian Holstein population to address country-specific needs and market demands.					
30131088	6	88	dep	additive	1036:1043	arg1	genetic					1045:1051	genetic	1045:1051	genetic	1045:1051	Random effects were animal additive genetic, within and across lactation permanent environment and the residual.					
30131088	7	89	theme	lactation	1194:1202	arg1	peak					1186:1189	the peak	1182:1189	the peak of lactation	1182:1202	Lactation curves of LP and LY increased from parturition to the peak of lactation and decreased thereafter, mirroring the typical curve of milk yield.					
30131088	11	90	dep	moderate	1784:1791	arg1	strong					1796:1801	strong	1796:1801	strong	1796:1801	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	90	dep	moderate	1784:1791	arg1	to					1793:1794	to	1793:1794	to	1793:1794	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	7	91	theme	yield	1266:1270	arg1	curve					1252:1256	the typical curve	1240:1256	the typical curve of milk yield	1240:1270	Lactation curves of LP and LY increased from parturition to the peak of lactation and decreased thereafter, mirroring the typical curve of milk yield.					
30131088	10	92	theme	Genetic	1473:1479	arg1	correlations					1481:1492	Genetic correlations	1473:1492	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point	1473:1556	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	10	92	theme	Genetic	1473:1479	arg1	a					1497:1497	r a	1495:1497	r a	1495:1497	Genetic correlations (r a) of LP with somatic cell score, LY and milk freezing point were -0.22±0.08, 0.28±0.08 and -0.46±0.05, respectively.					
30131088	4	93	theme	production	743:752	arg1	traits					754:759	production traits	743:759	production traits	743:759	Heritabilities of LP and lactose yield (LY) were estimated through single-trait repeatability animal models, whereas genetic and phenotypic correlations of LP and LY with milk composition and production traits, somatic cell score and milk freezing point were estimated using bivariate models.					
30131088	0	94	with	lactose	39:45	arg1	traits					91:96	traditional milk traits	74:96	traditional milk traits	74:96	Heritability and repeatability of milk lactose and its relationships with traditional milk traits, somatic cell score and freezing point in Holstein cows.					
30131088	11	95	theme	protein	1749:1755	arg1	yield					1757:1761	protein yield	1749:1761	protein yield (r a=0.64±0.06)	1749:1777	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	11	95	theme	protein	1749:1755	arg1	a=0.64±0.06					1766:1776	r a=0.64±0.06	1764:1776	r a=0.64±0.06	1764:1776	Genetic relationships of LY with milk yield (r a=0.97±0.00), fat percentage (r a=-0.71±0.06), protein percentage (r a=-0.57±0.06) and protein yield (r a=0.64±0.06) were moderate to strong.					
30131088	1	96	theme	most	214:217	arg1	schemes					232:238	most herd-testing schemes	214:238	most herd-testing schemes	214:238	Lactose percentage (LP) in milk is currently determined in most herd-testing schemes, and globally, it is usually routinely recorded in the framework of the official milk recording procedures.					
30025964	5	0	theme	TBARS	1122:1126	arg1	method					1128:1133	the TBARS method	1118:1133	the TBARS method	1118:1133	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	3	1	theme	reaction	728:735	arg1	substances					737:746	thiobarbituric acid reaction substances	708:746	thiobarbituric acid reaction substances (TBARS)	708:754	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	3	1	theme	reaction	728:735	arg1	TBARS					749:753	TBARS	749:753	TBARS	749:753	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	4	2	theme	lipid	862:866	arg1	oxidation					868:876	the lipid oxidation	858:876	the lipid oxidation of beef meat during 7 days of storage	858:914	HT added at 0.5% to the film with beeswax suppressed the lipid oxidation of beef meat during 7 days of storage at 4 °C, possibly by the combined effect of acting as an oxygen barrier and the specific antioxidant activity.					
30025964	4	3	theme	beef	881:884	arg1	meat					886:889	beef meat	881:889	beef meat	881:889	HT added at 0.5% to the film with beeswax suppressed the lipid oxidation of beef meat during 7 days of storage at 4 °C, possibly by the combined effect of acting as an oxygen barrier and the specific antioxidant activity.					
30025964	3	4	dep	reduced	649:655	arg1	compared					756:763	compared	756:763	compared with control film without antioxidants	756:802	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	5	5	from	film	1110:1113	arg1	method					1128:1133	the TBARS method	1118:1133	the TBARS method	1118:1133	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	4	6	theme	antioxidant	1005:1015	arg1	activity					1017:1024	the specific antioxidant activity	992:1024	the specific antioxidant activity	992:1024	HT added at 0.5% to the film with beeswax suppressed the lipid oxidation of beef meat during 7 days of storage at 4 °C, possibly by the combined effect of acting as an oxygen barrier and the specific antioxidant activity.					
30025964	1	7	theme	present	268:274	arg1	antioxidants					245:256	two antioxidants	241:256	two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG),	241:343	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	4	8	theme	storage	908:914	arg1	7 days					898:903	7 days	898:903	7 days of storage	898:914	HT added at 0.5% to the film with beeswax suppressed the lipid oxidation of beef meat during 7 days of storage at 4 °C, possibly by the combined effect of acting as an oxygen barrier and the specific antioxidant activity.					
30025964	3	9	theme	oxidation	674:682	arg1	products					684:691	oxidation products	674:691	oxidation products	674:691	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	1	10	theme	refrigerated	426:437	arg1	storage					439:445	refrigerated storage	426:445	refrigerated storage	426:445	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	4	11	theme	specific	996:1003	arg1	activity					1017:1024	the specific antioxidant activity	992:1024	the specific antioxidant activity	992:1024	HT added at 0.5% to the film with beeswax suppressed the lipid oxidation of beef meat during 7 days of storage at 4 °C, possibly by the combined effect of acting as an oxygen barrier and the specific antioxidant activity.					
30025964	4	12	theme	combined	941:948	arg1	effect					950:955	the combined effect	937:955	the combined effect of acting as an oxygen barrier and the specific antioxidant activity	937:1024	HT added at 0.5% to the film with beeswax suppressed the lipid oxidation of beef meat during 7 days of storage at 4 °C, possibly by the combined effect of acting as an oxygen barrier and the specific antioxidant activity.					
30025964	1	13	theme	pectin-fish	350:360	arg1	film					377:380	a pectin-fish gelatin edible film	348:380	a pectin-fish gelatin edible film	348:380	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	14	theme	research	186:193	arg1	objective					168:176	The objective	164:176	The objective of this research	164:193	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	0	15	theme	beef	125:128	arg1	meat					130:133	beef meat	125:133	beef meat	125:133	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	1	16	theme	gelatin	362:368	arg1	film					377:380	a pectin-fish gelatin edible film	348:380	a pectin-fish gelatin edible film	348:380	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	5	17	theme	plasticizer	1047:1057	arg1	agents					1059:1064	plasticizer agents	1047:1064	plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method	1047:1133	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	5	17	theme	plasticizer	1047:1057	arg1	sorbitol					1080:1087	sorbitol	1080:1087	sorbitol	1080:1087	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	5	17	theme	plasticizer	1047:1057	arg1	glycerol					1067:1074	glycerol	1067:1074	glycerol	1067:1074	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	2	18	theme	composite	454:462	arg1	film					464:467	A new composite film	448:467	A new composite film that included beeswax	448:489	A new composite film that included beeswax was also prepared, resulting in a reduction in the film's oxygen permeability.					
30025964	1	19	theme	edible	370:375	arg1	film					377:380	a pectin-fish gelatin edible film	348:380	a pectin-fish gelatin edible film	348:380	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	3	20	theme	thiobarbituric	708:721	arg1	substances					737:746	thiobarbituric acid reaction substances	708:746	thiobarbituric acid reaction substances (TBARS)	708:754	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	3	20	theme	thiobarbituric	708:721	arg1	TBARS					749:753	TBARS	749:753	TBARS	749:753	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	3	21	theme	substances	737:746	arg1	form					700:703	the form	696:703	the form of thiobarbituric acid reaction substances (TBARS)	696:754	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	0	22	from	3,4-dihydroxyphenylglycol	96:120	arg1	meat					130:133	beef meat	125:133	beef meat	125:133	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	0	23	dep	hydroxytyrosol	77:90	arg1	the					54:56	the	54:56	the	54:56	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	3	24	theme	containing	625:634	arg1	antioxidants					636:647	film containing antioxidants	620:647	film containing antioxidants	620:647	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	0	25	theme	pectin-fish	17:27	arg1	films					37:41	edible pectin-fish gelatin films	10:41	edible pectin-fish gelatin films	10:41	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	0	26	theme	refrigerated	142:153	arg1	storage					155:161	refrigerated storage	142:161	refrigerated storage	142:161	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	0	27	theme	edible	10:15	arg1	films					37:41	edible pectin-fish gelatin films	10:41	edible pectin-fish gelatin films	10:41	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	3	28	theme	acid	723:726	arg1	substances					737:746	thiobarbituric acid reaction substances	708:746	thiobarbituric acid reaction substances (TBARS)	708:754	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	3	28	theme	acid	723:726	arg1	TBARS					749:753	TBARS	749:753	TBARS	749:753	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	1	29	from	olives	279:284	arg1	present					268:274	present	268:274	present	268:274	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	3	30	theme	film	620:623	arg1	antioxidants					636:647	film containing antioxidants	620:647	film containing antioxidants	620:647	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	0	31	theme	films	37:41	arg1	Effect					0:5	Effect	0:5	Effect of edible pectin-fish gelatin films	0:41	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	3	32	theme	meat	594:597	arg1	samples					599:605	the meat samples	590:605	the meat samples wrapped with film containing antioxidants	590:647	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	0	33	from	hydroxytyrosol	77:90	arg1	meat					130:133	beef meat	125:133	beef meat	125:133	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	0	34	theme	gelatin	29:35	arg1	films					37:41	edible pectin-fish gelatin films	10:41	edible pectin-fish gelatin films	10:41	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	5	35	dep	agents	1059:1064	arg1	agents					1059:1064	plasticizer agents	1047:1064	plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method	1047:1133	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	5	35	dep	agents	1059:1064	arg1	sorbitol					1080:1087	sorbitol	1080:1087	sorbitol	1080:1087	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	5	35	dep	agents	1059:1064	arg1	glycerol					1067:1074	glycerol	1067:1074	glycerol	1067:1074	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	1	36	from	present	268:274	arg1	3,4-dihydroxyphenylglycol					311:335	3,4-dihydroxyphenylglycol	311:335	3,4-dihydroxyphenylglycol (DHPG)	311:342	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	36	from	present	268:274	arg1	hydroxytyrosol					287:300	hydroxytyrosol	287:300	hydroxytyrosol (HT)	287:305	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	36	from	present	268:274	arg1	olives					279:284	olives	279:284	olives	279:284	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	2	37	theme	new	450:452	arg1	film					464:467	A new composite film	448:467	A new composite film that included beeswax	448:489	A new composite film that included beeswax was also prepared, resulting in a reduction in the film's oxygen permeability.					
30025964	4	38	theme	oxygen	973:978	arg1	barrier					980:986	an oxygen barrier	970:986	an oxygen barrier	970:986	HT added at 0.5% to the film with beeswax suppressed the lipid oxidation of beef meat during 7 days of storage at 4 °C, possibly by the combined effect of acting as an oxygen barrier and the specific antioxidant activity.					
30025964	2	39	theme	oxygen	549:554	arg1	permeability					556:567	the film's oxygen permeability	538:567	the film's oxygen permeability	538:567	A new composite film that included beeswax was also prepared, resulting in a reduction in the film's oxygen permeability.					
30025964	5	40	theme	agents	1059:1064	arg1	interference					1031:1042	The interference	1027:1042	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method	1027:1133	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	4	41	theme	meat	886:889	arg1	oxidation					868:876	the lipid oxidation	858:876	the lipid oxidation of beef meat during 7 days of storage	858:914	HT added at 0.5% to the film with beeswax suppressed the lipid oxidation of beef meat during 7 days of storage at 4 °C, possibly by the combined effect of acting as an oxygen barrier and the specific antioxidant activity.					
30025964	1	42	theme	raw	405:407	arg1	meat					414:417	raw beef meat	405:417	raw beef meat	405:417	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	43	attach	present	268:274	arg1	3,4-dihydroxyphenylglycol					311:335	3,4-dihydroxyphenylglycol	311:335	3,4-dihydroxyphenylglycol (DHPG)	311:342	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	43	attach	present	268:274	arg2	antioxidants					245:256	two antioxidants	241:256	two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG),	241:343	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	43	attach	present	268:274	arg1	hydroxytyrosol					287:300	hydroxytyrosol	287:300	hydroxytyrosol (HT)	287:305	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	43	attach	present	268:274	arg1	olives					279:284	olives	279:284	olives	279:284	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	44	from	effect	215:220	arg1	preservation					389:400	the preservation	385:400	the preservation of raw beef meat during refrigerated storage	385:445	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	45	theme	addition	229:236	arg1	effect					215:220	the effect	211:220	the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage	211:445	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	1	46	theme	beef	409:412	arg1	meat					414:417	raw beef meat	405:417	raw beef meat	405:417	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	0	47	theme	antioxidants	64:75	arg1	hydroxytyrosol					77:90	olive antioxidants hydroxytyrosol	58:90	olive antioxidants hydroxytyrosol	58:90	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	1	48	theme	meat	414:417	arg1	preservation					389:400	the preservation	385:400	the preservation of raw beef meat during refrigerated storage	385:445	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
30025964	4	49	with	film	829:832	arg1	beeswax					839:845	beeswax	839:845	beeswax	839:845	HT added at 0.5% to the film with beeswax suppressed the lipid oxidation of beef meat during 7 days of storage at 4 °C, possibly by the combined effect of acting as an oxygen barrier and the specific antioxidant activity.					
30025964	3	50	theme	control	770:776	arg1	film					778:781	control film	770:781	control film without antioxidants	770:802	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	0	51	theme	olive	58:62	arg1	hydroxytyrosol					77:90	olive antioxidants hydroxytyrosol	58:90	olive antioxidants hydroxytyrosol	58:90	Effect of edible pectin-fish gelatin films containing the olive antioxidants hydroxytyrosol and 3,4-dihydroxyphenylglycol on beef meat during refrigerated storage.					
30025964	3	52	theme	products	684:691	arg1	formation					661:669	the formation	657:669	the formation of oxidation products	657:691	Results showed that the meat samples wrapped with film containing antioxidants reduced the formation of oxidation products in the form of thiobarbituric acid reaction substances (TBARS) compared with control film without antioxidants.					
30025964	2	53	from	reduction	525:533	arg1	permeability					556:567	the film's oxygen permeability	538:567	the film's oxygen permeability	538:567	A new composite film that included beeswax was also prepared, resulting in a reduction in the film's oxygen permeability.					
30025964	5	54	theme	first	1154:1158	arg1	time					1160:1163	the first time	1150:1163	the first time	1150:1163	The interference of plasticizer agents (glycerol and sorbitol) incorporated to the film on the TBARS method was showed for the first time.					
30025964	1	55	theme	antioxidants	245:256	arg1	addition					229:236	the addition	225:236	the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film	225:380	The objective of this research was to evaluate the effect of the addition of two antioxidants naturally present in olives, hydroxytyrosol (HT) and 3,4-dihydroxyphenylglycol (DHPG), to a pectin-fish gelatin edible film on the preservation of raw beef meat during refrigerated storage.					
31253330	0	0	theme	acid	93:96	arg1	bacteria					98:105	lactic acid bacteria	86:105	lactic acid bacteria	86:105	An electricalchemical method to detect the branch-chain aminotransferases activity in lactic acid bacteria.					
31253330	6	1	from	activity	919:926	arg1	culture					961:967	the LAB culture	953:967	the LAB culture	953:967	The system effectively and sensitively detected the BCAT activity by NADH concentration in the LAB culture, comparing with the optical method.					
31253330	7	2	theme	culture	1009:1015	arg1	condition					1017:1025	The culture condition	1005:1025	The culture condition of LAB	1005:1032	The culture condition of LAB was optimized by using this system, evidencing that established method was available to detect the BCAT activity of LAB.					
31253330	1	3	theme	acid	248:251	arg1	bacteria					253:260	lactic acid bacteria	241:260	lactic acid bacteria (LAB)	241:266	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	1	3	theme	acid	248:251	arg1	LAB					263:265	LAB	263:265	LAB	263:265	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	2	4	theme	GCE	446:448	arg1	surface					451:457	the glassy carbon electrode (GCE) surface	417:457	the glassy carbon electrode (GCE) surface	417:457	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	7	5	theme	established	1086:1096	arg1	method					1098:1103	established method	1086:1103	established method	1086:1103	The culture condition of LAB was optimized by using this system, evidencing that established method was available to detect the BCAT activity of LAB.					
31253330	7	6	theme	LAB	1030:1032	arg1	condition					1017:1025	The culture condition	1005:1025	The culture condition of LAB	1005:1032	The culture condition of LAB was optimized by using this system, evidencing that established method was available to detect the BCAT activity of LAB.					
31253330	3	7	theme	cyclic	592:597	arg1	TEM					612:614	TEM	612:614	TEM	612:614	The composite was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (TEM).					
31253330	3	7	theme	cyclic	592:597	arg1	voltammetry					599:609	cyclic voltammetry	592:609	cyclic voltammetry (TEM)	592:615	The composite was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (TEM).					
31253330	7	8	theme	LAB	1150:1152	arg1	activity					1138:1145	the BCAT activity	1129:1145	the BCAT activity of LAB	1129:1152	The culture condition of LAB was optimized by using this system, evidencing that established method was available to detect the BCAT activity of LAB.					
31253330	4	9	theme	GCE	731:733	arg1	electrode					735:743	the GCE electrode	727:743	the GCE electrode	727:743	Results indicated that the MWCNTs-CS/GCE electrode exhibited higher stability and sensitivity, compared with the GCE electrode.					
31253330	6	10	theme	LAB	957:959	arg1	culture					961:967	the LAB culture	953:967	the LAB culture	953:967	The system effectively and sensitively detected the BCAT activity by NADH concentration in the LAB culture, comparing with the optical method.					
31253330	0	11	theme	branch-chain	43:54	arg1	aminotransferases					56:72	the branch-chain aminotransferases	39:72	the branch-chain aminotransferases	39:72	An electricalchemical method to detect the branch-chain aminotransferases activity in lactic acid bacteria.					
31253330	2	12	with	chitosan	288:295	arg1	MWCNTs					338:343	MWCNTs	338:343	MWCNTs	338:343	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	2	12	with	chitosan	288:295	arg1	nanotubes					327:335	multi-walled carbon nanotubes	307:335	multi-walled carbon nanotubes (MWCNTs)	307:344	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	5	13	theme	response	834:841	arg1	limit					843:847	the response limit	830:847	the response limit	830:847	The linear response for nicotinamide adenine dinucleotide (NADH) was 1.0-9.0 μM and the response limit was 0.12 µM.					
31253330	0	14	theme	electricalchemical	3:20	arg1	method					22:27	An electricalchemical method	0:27	An electricalchemical method to detect the branch-chain aminotransferases	0:72	An electricalchemical method to detect the branch-chain aminotransferases activity in lactic acid bacteria.					
31253330	0	15	theme	lactic	86:91	arg1	bacteria					98:105	lactic acid bacteria	86:105	lactic acid bacteria	86:105	An electricalchemical method to detect the branch-chain aminotransferases activity in lactic acid bacteria.					
31253330	7	16	theme	BCAT	1133:1136	arg1	activity					1138:1145	the BCAT activity	1129:1145	the BCAT activity of LAB	1129:1152	The culture condition of LAB was optimized by using this system, evidencing that established method was available to detect the BCAT activity of LAB.					
31253330	1	17	theme	branched-chain	179:192	arg1	activity					229:236	the branched-chain amino acid aminotransferase (BCAT) activity	175:236	the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB)	175:266	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	1	18	theme	amino	194:198	arg1	activity					229:236	the branched-chain amino acid aminotransferase (BCAT) activity	175:236	the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB)	175:266	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	2	19	theme	chitosan	288:295	arg1	chitosan					288:295	chitosan	288:295	chitosan (CS) with multi-walled carbon nanotubes (MWCNTs)	288:344	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	2	19	theme	chitosan	288:295	arg1	nanocomposite					271:283	A nanocomposite	269:283	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs)	269:344	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	1	20	theme	acid	200:203	arg1	activity					229:236	the branched-chain amino acid aminotransferase (BCAT) activity	175:236	the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB)	175:266	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	1	21	theme	aminotransferase	205:220	arg1	activity					229:236	the branched-chain amino acid aminotransferase (BCAT) activity	175:236	the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB)	175:266	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	5	22	theme	linear	750:755	arg1	response					757:764	The linear response	746:764	The linear response for nicotinamide adenine dinucleotide (NADH)	746:809	The linear response for nicotinamide adenine dinucleotide (NADH) was 1.0-9.0 μM and the response limit was 0.12 µM.					
31253330	4	23	theme	higher	679:684	arg1	stability					686:694	higher stability	679:694	higher stability	679:694	Results indicated that the MWCNTs-CS/GCE electrode exhibited higher stability and sensitivity, compared with the GCE electrode.					
31253330	6	24	theme	NADH	931:934	arg1	concentration					936:948	NADH concentration	931:948	NADH concentration	931:948	The system effectively and sensitively detected the BCAT activity by NADH concentration in the LAB culture, comparing with the optical method.					
31253330	2	25	theme	composite	371:379	arg1	solution					381:388	the composite solution	367:388	the composite solution	367:388	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	2	26	theme	electrode	435:443	arg1	surface					451:457	the glassy carbon electrode (GCE) surface	417:457	the glassy carbon electrode (GCE) surface	417:457	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	2	27	theme	electrochemical	491:505	arg1	biosensor					507:515	an electrochemical biosensor	488:515	an electrochemical biosensor	488:515	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	1	28	theme	BCAT	223:226	arg1	activity					229:236	the branched-chain amino acid aminotransferase (BCAT) activity	175:236	the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB)	175:266	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	2	29	theme	carbon	428:433	arg1	surface					451:457	the glassy carbon electrode (GCE) surface	417:457	the glassy carbon electrode (GCE) surface	417:457	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	5	30	theme	nicotinamide	770:781	arg1	dinucleotide					791:802	nicotinamide adenine dinucleotide	770:802	nicotinamide adenine dinucleotide (NADH)	770:809	The linear response for nicotinamide adenine dinucleotide (NADH) was 1.0-9.0 μM and the response limit was 0.12 µM.					
31253330	5	30	theme	nicotinamide	770:781	arg1	NADH					805:808	NADH	805:808	NADH	805:808	The linear response for nicotinamide adenine dinucleotide (NADH) was 1.0-9.0 μM and the response limit was 0.12 µM.					
31253330	0	31	from	activity	74:81	arg1	bacteria					98:105	lactic acid bacteria	86:105	lactic acid bacteria	86:105	An electricalchemical method to detect the branch-chain aminotransferases activity in lactic acid bacteria.					
31253330	3	32	theme	scanning	553:560	arg1	microscopy					571:580	scanning electron microscopy	553:580	scanning electron microscopy (SEM)	553:586	The composite was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (TEM).					
31253330	3	32	theme	scanning	553:560	arg1	SEM					583:585	SEM	583:585	SEM	583:585	The composite was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (TEM).					
31253330	2	33	theme	carbon	320:325	arg1	MWCNTs					338:343	MWCNTs	338:343	MWCNTs	338:343	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	2	33	theme	carbon	320:325	arg1	nanotubes					327:335	multi-walled carbon nanotubes	307:335	multi-walled carbon nanotubes (MWCNTs)	307:344	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	2	34	theme	glassy	421:426	arg1	surface					451:457	the glassy carbon electrode (GCE) surface	417:457	the glassy carbon electrode (GCE) surface	417:457	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	6	35	theme	BCAT	914:917	arg1	activity					919:926	the BCAT activity	910:926	the BCAT activity by NADH concentration in the LAB culture	910:967	The system effectively and sensitively detected the BCAT activity by NADH concentration in the LAB culture, comparing with the optical method.					
31253330	3	36	theme	electron	562:569	arg1	microscopy					571:580	scanning electron microscopy	553:580	scanning electron microscopy (SEM)	553:586	The composite was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (TEM).					
31253330	3	36	theme	electron	562:569	arg1	SEM					583:585	SEM	583:585	SEM	583:585	The composite was characterized by scanning electron microscopy (SEM) and cyclic voltammetry (TEM).					
31253330	2	37	theme	multi-walled	307:318	arg1	MWCNTs					338:343	MWCNTs	338:343	MWCNTs	338:343	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	2	37	theme	multi-walled	307:318	arg1	nanotubes					327:335	multi-walled carbon nanotubes	307:335	multi-walled carbon nanotubes (MWCNTs)	307:344	A nanocomposite of chitosan (CS) with multi-walled carbon nanotubes (MWCNTs) was synthesized, and the composite solution were uniformly spread over the glassy carbon electrode (GCE) surface by drop-casting to fabricate an electrochemical biosensor.					
31253330	1	38	from	activity	229:236	arg1	bacteria					253:260	lactic acid bacteria	241:260	lactic acid bacteria (LAB)	241:266	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	1	38	from	activity	229:236	arg1	LAB					263:265	LAB	263:265	LAB	263:265	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	0	39	dep	method	22:27	arg1	detect					32:37	detect	32:37	to detect the branch-chain aminotransferases	29:72	An electricalchemical method to detect the branch-chain aminotransferases activity in lactic acid bacteria.					
31253330	1	40	theme	electrochemical	126:140	arg1	system					142:147	an electrochemical system	123:147	an electrochemical system	123:147	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	6	41	theme	optical	989:995	arg1	method					997:1002	the optical method	985:1002	the optical method	985:1002	The system effectively and sensitively detected the BCAT activity by NADH concentration in the LAB culture, comparing with the optical method.					
31253330	4	42	theme	MWCNTs-CS/GCE	645:657	arg1	electrode					659:667	the MWCNTs-CS/GCE electrode	641:667	the MWCNTs-CS/GCE electrode	641:667	Results indicated that the MWCNTs-CS/GCE electrode exhibited higher stability and sensitivity, compared with the GCE electrode.					
31253330	5	43	theme	adenine	783:789	arg1	dinucleotide					791:802	nicotinamide adenine dinucleotide	770:802	nicotinamide adenine dinucleotide (NADH)	770:809	The linear response for nicotinamide adenine dinucleotide (NADH) was 1.0-9.0 μM and the response limit was 0.12 µM.					
31253330	5	43	theme	adenine	783:789	arg1	NADH					805:808	NADH	805:808	NADH	805:808	The linear response for nicotinamide adenine dinucleotide (NADH) was 1.0-9.0 μM and the response limit was 0.12 µM.					
31253330	1	44	theme	lactic	241:246	arg1	bacteria					253:260	lactic acid bacteria	241:260	lactic acid bacteria (LAB)	241:266	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
31253330	1	44	theme	lactic	241:246	arg1	LAB					263:265	LAB	263:265	LAB	263:265	In this study, an electrochemical system was established to detect the branched-chain amino acid aminotransferase (BCAT) activity in lactic acid bacteria (LAB).					
29735050	10	0	theme	CSLM	1472:1475	arg1	images					1477:1482	CSLM images	1472:1482	CSLM images	1472:1482	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	9	1	theme	SDS-insoluble	1339:1351	arg1	result					1378:1383	a result	1376:1383	a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones	1376:1469	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	9	1	theme	SDS-insoluble	1339:1351	arg1	aggregates					1361:1370	larger SDS-insoluble protein aggregates	1332:1370	larger SDS-insoluble protein aggregates	1332:1370	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	10	2	theme	extruded	1499:1506	arg1	flour					1512:1516	extruded pea flour	1499:1516	extruded pea flour	1499:1516	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	9	3	theme	bonds	1446:1450	arg1	creation					1425:1432	creation	1425:1432	creation of covalent bonds other than SS ones	1425:1469	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	1	4	theme	yellow	91:96	arg1	flour					102:106	Dehulled yellow pea flour	82:106	Dehulled yellow pea flour	82:106	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	8	5	theme	protein	1209:1215	arg1	solubility					1217:1226	protein solubility	1209:1226	protein solubility	1209:1226	The SME impact on protein solubility followed similar trends.					
29735050	1	6	from	contents	205:212	arg1	extruder					176:183	a twin-screw extruder	163:183	a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	163:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	10	7	theme	dispersed	1563:1571	arg1	aggregates					1587:1596	dispersed small protein aggregates	1563:1596	dispersed small protein aggregates	1563:1596	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	7	8	dep	[125	1149:1152	arg1	146 °C					1155:1160	146 °C	1155:1160	146 °C	1155:1160	These trends could be described by sigmoid models, which allowed determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content.					
29735050	11	9	theme	pea	1720:1722	arg1	flour					1724:1728	pea flour	1720:1728	pea flour	1720:1728	This morphology would govern the expansion of pea flour by extrusion.					
29735050	6	10	theme	proteins	993:1000	arg1	%					970:970	5 to 45% (R2 = 0.75)	963:982	5 to 45% (R2 = 0.75) of total proteins	963:1000	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	6	10	theme	proteins	993:1000	arg1	proteins					993:1000	total proteins	987:1000	total proteins	987:1000	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	2	11	theme	density	416:422	arg1	expansion					425:433	density (expansion)	416:434	density (expansion)	416:434	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	11	theme	density	416:422	arg1	crystallinity					437:449	crystallinity	437:449	crystallinity (X-ray diffraction)	437:469	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	11	theme	density	416:422	arg1	enthalpy					487:494	gelatinisation enthalpy	472:494	gelatinisation enthalpy (DSC)	472:500	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	11	theme	density	416:422	arg1	solubility					510:519	starch solubility	503:519	starch solubility in water and protein solubility in SDS and DTE (SE-HPLC)	503:576	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	1	12	dep	contents	205:212	arg1	50-1200 kJ/kg					304:316	50-1200 kJ/kg	304:316	50-1200 kJ/kg	304:316	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	12	dep	contents	205:212	arg1	T					252:252	product temperature T	232:252	product temperature T (115-165 °C)	232:265	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	12	dep	contents	205:212	arg1	%					223:223	18-35% w.b.	218:228	18-35% w.b.	218:228	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	12	dep	contents	205:212	arg1	115-165 °C					255:264	115-165 °C	255:264	115-165 °C	255:264	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	12	dep	contents	205:212	arg1	contents					205:212	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	12	dep	contents	205:212	arg1	SME					299:301	specific mechanical energy SME	272:301	specific mechanical energy SME (50-1200 kJ/kg)	272:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	12	dep	contents	205:212	arg1	MC					214:215	MC	214:215	MC (18-35% w.b.)	214:229	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	0	13	theme	pea	61:63	arg1	extrusion					71:79	pea flour extrusion	61:79	pea flour extrusion	61:79	Multi-scale structural changes of starch and proteins during pea flour extrusion.					
29735050	10	14	theme	protein	1579:1585	arg1	aggregates					1587:1596	dispersed small protein aggregates	1563:1596	dispersed small protein aggregates	1563:1596	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	9	15	theme	network	1299:1305	arg1	creation					1279:1286	the creation	1275:1286	the creation of protein network by SS bonds	1275:1317	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	9	16	theme	SS	1463:1464	arg1	ones					1466:1469	SS ones	1463:1469	SS ones	1463:1469	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	6	17	dep	165 °C	825:830	arg1	to					822:823	to	822:823	to	822:823	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	6	18	from	SDS	862:864	arg1	soluble					851:857	soluble	851:857	soluble	851:857	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	5	19	dep	50	785:786	arg1	to					782:783	to	782:783	to	782:783	Its solubility in water augmented up to 50%.					
29735050	5	20	from	solubility	749:758	arg1	water					763:767	water	763:767	water	763:767	Its solubility in water augmented up to 50%.					
29735050	9	21	theme	SS	1310:1311	arg1	bonds					1313:1317	SS bonds	1310:1317	SS bonds	1310:1317	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	1	22	dep	%	223:223	arg1	w.b.					225:228	w.b.	225:228	w.b.	225:228	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	23	dep	%	113:113	arg1	starch					115:120	starch	115:120	48.2% starch	109:120	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	2	24	theme	expansion	425:433	arg1	measurements					400:411	measurements	400:411	measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC)	400:576	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	1	25	theme	mechanical	281:290	arg1	50-1200 kJ/kg					304:316	50-1200 kJ/kg	304:316	50-1200 kJ/kg	304:316	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	25	theme	mechanical	281:290	arg1	contents					205:212	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	25	theme	mechanical	281:290	arg1	SME					299:301	specific mechanical energy SME	272:301	specific mechanical energy SME (50-1200 kJ/kg)	272:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	7	26	theme	solubility	1122:1131	arg1	changes					1103:1109	changes	1103:1109	changes of protein solubility in the interval [125, 146 °C], whatever moisture content	1103:1188	These trends could be described by sigmoid models, which allowed determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content.					
29735050	6	27	from	soluble	851:857	arg1	SDS					862:864	SDS	862:864	SDS from 95 to 35% (R2 = 0.83) of total proteins	862:909	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	1	28	dep	%	127:127	arg1	proteins					129:136	proteins	129:136	23.4% proteins	123:136	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	0	29	theme	Multi-scale	0:10	arg1	changes					23:29	Multi-scale structural changes	0:29	Multi-scale structural changes of starch and proteins during pea flour extrusion.	0:80	Multi-scale structural changes of starch and proteins during pea flour extrusion.					
29735050	9	30	theme	protein	1353:1359	arg1	result					1378:1383	a result	1376:1383	a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones	1376:1469	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	9	30	theme	protein	1353:1359	arg1	aggregates					1361:1370	larger SDS-insoluble protein aggregates	1332:1370	larger SDS-insoluble protein aggregates	1332:1370	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	2	31	theme	pea	351:353	arg1	flour					355:359	extruded pea flour	342:359	extruded pea flour	342:359	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	32	theme	Structural	320:329	arg1	changes					331:337	Structural changes	320:337	Structural changes of extruded pea flour	320:359	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	0	33	theme	starch	34:39	arg1	changes					23:29	Multi-scale structural changes	0:29	Multi-scale structural changes of starch and proteins during pea flour extrusion.	0:80	Multi-scale structural changes of starch and proteins during pea flour extrusion.					
29735050	7	34	theme	onset	1080:1084	arg1	temperatures					1086:1097	determining onset temperatures	1068:1097	determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content	1068:1188	These trends could be described by sigmoid models, which allowed determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content.					
29735050	10	35	theme	bi-continuous	1603:1615	arg1	matrix					1617:1622	a bi-continuous matrix	1601:1622	a bi-continuous matrix of large protein aggregates and amorphous starch	1601:1671	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	4	36	dep	amorphous	704:712	arg1	conditions					733:742	extrusion conditions	723:742	extrusion conditions	723:742	DSC and XRD results showed that starch was amorphous whatever extrusion conditions.					
29735050	1	37	theme	temperature	240:250	arg1	contents					205:212	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	37	theme	temperature	240:250	arg1	115-165 °C					255:264	115-165 °C	255:264	115-165 °C	255:264	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	37	theme	temperature	240:250	arg1	T					252:252	product temperature T	232:252	product temperature T (115-165 °C)	232:265	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	8	38	theme	SME	1195:1197	arg1	impact					1199:1204	The SME impact	1191:1204	The SME impact on protein solubility	1191:1226	The SME impact on protein solubility followed similar trends.					
29735050	1	39	theme	twin-screw	165:174	arg1	extruder					176:183	a twin-screw extruder	163:183	a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	163:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	7	40	from	changes	1103:1109	arg1	[125					1149:1152	[125	1149:1152	[125	1149:1152	These trends could be described by sigmoid models, which allowed determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content.					
29735050	10	41	theme	protein	1633:1639	arg1	aggregates					1641:1650	large protein aggregates	1627:1650	large protein aggregates	1627:1650	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	7	42	theme	sigmoid	1038:1044	arg1	models					1046:1051	sigmoid models	1038:1051	sigmoid models	1038:1051	These trends could be described by sigmoid models, which allowed determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content.					
29735050	3	43	theme	Foam	579:582	arg1	density					584:590	Foam density	579:590	Foam density	579:590	Foam density dropped from 820 to 85 kg/m3 with increase in SME and T (R2 ≥ 0.78).					
29735050	1	44	theme	moisture	196:203	arg1	contents					205:212	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	44	theme	moisture	196:203	arg1	SME					299:301	specific mechanical energy SME	272:301	specific mechanical energy SME (50-1200 kJ/kg)	272:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	44	theme	moisture	196:203	arg1	MC					214:215	MC	214:215	MC (18-35% w.b.)	214:229	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	44	theme	moisture	196:203	arg1	T					252:252	product temperature T	232:252	product temperature T (115-165 °C)	232:265	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	6	45	theme	proteins	902:909	arg1	%					879:879	95 to 35%	871:879	95 to 35% (R2 = 0.83) of total proteins	871:909	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	6	45	theme	proteins	902:909	arg1	proteins					902:909	total proteins	896:909	total proteins	896:909	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	6	45	theme	proteins	902:909	arg1	R2 = 0.83					882:890	R2 = 0.83	882:890	R2 = 0.83	882:890	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	10	46	theme	starch	1666:1671	arg1	matrix					1617:1622	a bi-continuous matrix	1601:1622	a bi-continuous matrix of large protein aggregates and amorphous starch	1601:1671	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	4	47	theme	extrusion	723:731	arg1	conditions					733:742	extrusion conditions	723:742	extrusion conditions	723:742	DSC and XRD results showed that starch was amorphous whatever extrusion conditions.					
29735050	9	48	dep	SDS-insoluble	1339:1351	arg1	larger					1332:1337	larger	1332:1337	larger	1332:1337	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	10	49	theme	pea	1508:1510	arg1	flour					1512:1516	extruded pea flour	1499:1516	extruded pea flour	1499:1516	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	0	50	theme	proteins	45:52	arg1	changes					23:29	Multi-scale structural changes	0:29	Multi-scale structural changes of starch and proteins during pea flour extrusion.	0:80	Multi-scale structural changes of starch and proteins during pea flour extrusion.					
29735050	9	51	theme	covalent	1437:1444	arg1	bonds					1446:1450	covalent bonds	1437:1450	covalent bonds other than SS ones	1437:1469	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	2	52	theme	starch	503:508	arg1	solubility					510:519	starch solubility	503:519	starch solubility in water and protein solubility in SDS and DTE (SE-HPLC)	503:576	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	52	theme	starch	503:508	arg1	expansion					425:433	density (expansion)	416:434	density (expansion)	416:434	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	6	53	from	%	879:879	arg1	SDS					862:864	SDS	862:864	SDS from 95 to 35% (R2 = 0.83) of total proteins	862:909	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	10	54	theme	composite	1524:1532	arg1	morphology					1534:1543	a composite morphology	1522:1543	a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch	1522:1671	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	8	55	from	impact	1199:1204	arg1	solubility					1217:1226	protein solubility	1209:1226	protein solubility	1209:1226	The SME impact on protein solubility followed similar trends.					
29735050	2	56	theme	X-ray	452:456	arg1	crystallinity					437:449	crystallinity	437:449	crystallinity (X-ray diffraction)	437:469	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	56	theme	X-ray	452:456	arg1	diffraction					458:468	X-ray diffraction	452:468	X-ray diffraction	452:468	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	7	57	theme	moisture	1173:1180	arg1	content					1182:1188	moisture content	1173:1188	moisture content	1173:1188	These trends could be described by sigmoid models, which allowed determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content.					
29735050	6	58	dep	45	968:969	arg1	to					965:966	to	965:966	to	965:966	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	9	59	theme	other	1452:1456	arg1	bonds					1446:1450	covalent bonds	1437:1450	covalent bonds other than SS ones	1437:1469	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	10	60	theme	small	1573:1577	arg1	aggregates					1587:1596	dispersed small protein aggregates	1563:1596	dispersed small protein aggregates	1563:1596	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	6	61	dep	Increasing	790:799	arg1	decreased					832:840	decreased	832:840	decreased	832:840	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	2	62	theme	gelatinisation	472:485	arg1	expansion					425:433	density (expansion)	416:434	density (expansion)	416:434	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	62	theme	gelatinisation	472:485	arg1	DSC					497:499	DSC	497:499	DSC	497:499	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	62	theme	gelatinisation	472:485	arg1	enthalpy					487:494	gelatinisation enthalpy	472:494	gelatinisation enthalpy (DSC)	472:500	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	6	63	theme	total	987:991	arg1	proteins					993:1000	total proteins	987:1000	total proteins	987:1000	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	2	64	from	solubility	542:551	arg1	SE-HPLC					569:575	SE-HPLC	569:575	SE-HPLC	569:575	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	64	from	solubility	542:551	arg1	DTE					564:566	DTE	564:566	DTE (SE-HPLC)	564:576	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	64	from	solubility	542:551	arg1	SDS					556:558	SDS	556:558	SDS	556:558	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	0	65	theme	flour	65:69	arg1	extrusion					71:79	pea flour extrusion	61:79	pea flour extrusion	61:79	Multi-scale structural changes of starch and proteins during pea flour extrusion.					
29735050	9	66	theme	protein	1291:1297	arg1	network					1299:1305	protein network	1291:1305	protein network	1291:1305	These results suggest the creation of protein network by SS bonds, implicating larger SDS-insoluble protein aggregates, as a result of increasing T and SME, accompanied by creation of covalent bonds other than SS ones.					
29735050	1	67	dep	flour	102:106	arg1	%					113:113	48.2% starch	109:120	48.2% starch	109:120	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	67	dep	flour	102:106	arg1	%					127:127	23.4% proteins	123:136	23.4% proteins	123:136	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	67	dep	flour	102:106	arg1	d.b.					139:142	d.b.	139:142	d.b.	139:142	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	3	68	dep	85 kg/m3	612:619	arg1	to					609:610	to	609:610	to	609:610	Foam density dropped from 820 to 85 kg/m3 with increase in SME and T (R2 ≥ 0.78).					
29735050	7	69	dep	content	1182:1188	arg1	whatever					1164:1171	whatever	1164:1171	whatever	1164:1171	These trends could be described by sigmoid models, which allowed determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content.					
29735050	6	70	theme	soluble	851:857	arg1	proteins					842:849	proteins	842:849	proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins	842:909	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	10	71	contain	had	1518:1520	arg1	flour					1512:1516	extruded pea flour	1499:1516	extruded pea flour	1499:1516	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	10	71	contain	had	1518:1520	arg2	morphology					1534:1543	a composite morphology	1522:1543	a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch	1522:1671	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	7	72	theme	protein	1114:1120	arg1	solubility					1122:1131	protein solubility	1114:1131	protein solubility	1114:1131	These trends could be described by sigmoid models, which allowed determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content.					
29735050	2	73	theme	protein	534:540	arg1	solubility					542:551	protein solubility	534:551	protein solubility	534:551	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	1	74	theme	specific	272:279	arg1	50-1200 kJ/kg					304:316	50-1200 kJ/kg	304:316	50-1200 kJ/kg	304:316	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	74	theme	specific	272:279	arg1	contents					205:212	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	74	theme	specific	272:279	arg1	SME					299:301	specific mechanical energy SME	272:301	specific mechanical energy SME (50-1200 kJ/kg)	272:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	6	75	theme	total	896:900	arg1	proteins					902:909	total proteins	896:909	total proteins	896:909	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	6	76	dep	%	879:879	arg1	to					874:875	to	874:875	to	874:875	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	0	77	theme	structural	12:21	arg1	changes					23:29	Multi-scale structural changes	0:29	Multi-scale structural changes of starch and proteins during pea flour extrusion.	0:80	Multi-scale structural changes of starch and proteins during pea flour extrusion.					
29735050	1	78	theme	energy	292:297	arg1	50-1200 kJ/kg					304:316	50-1200 kJ/kg	304:316	50-1200 kJ/kg	304:316	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	78	theme	energy	292:297	arg1	contents					205:212	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	78	theme	energy	292:297	arg1	SME					299:301	specific mechanical energy SME	272:301	specific mechanical energy SME (50-1200 kJ/kg)	272:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	79	theme	Dehulled	82:89	arg1	flour					102:106	Dehulled yellow pea flour	82:106	Dehulled yellow pea flour	82:106	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	2	80	theme	flour	355:359	arg1	changes					331:337	Structural changes	320:337	Structural changes of extruded pea flour	320:359	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	3	81	from	increase	626:633	arg1	SME					638:640	SME	638:640	SME	638:640	Foam density dropped from 820 to 85 kg/m3 with increase in SME and T (R2 ≥ 0.78).					
29735050	3	81	from	increase	626:633	arg1	R2 ≥ 0.78					649:657	R2 ≥ 0.78	649:657	R2 ≥ 0.78	649:657	Foam density dropped from 820 to 85 kg/m3 with increase in SME and T (R2 ≥ 0.78).					
29735050	3	81	from	increase	626:633	arg1	T					646:646	T	646:646	T	646:646	Foam density dropped from 820 to 85 kg/m3 with increase in SME and T (R2 ≥ 0.78).					
29735050	1	82	theme	pea	98:100	arg1	flour					102:106	Dehulled yellow pea flour	82:106	Dehulled yellow pea flour	82:106	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	11	83	theme	flour	1724:1728	arg1	expansion					1707:1715	the expansion	1703:1715	the expansion of pea flour by extrusion	1703:1741	This morphology would govern the expansion of pea flour by extrusion.					
29735050	2	84	theme	extruded	342:349	arg1	flour					355:359	extruded pea flour	342:359	extruded pea flour	342:359	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	10	85	theme	large	1627:1631	arg1	aggregates					1641:1650	large protein aggregates	1627:1650	large protein aggregates	1627:1650	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	4	86	dep	conditions	733:742	arg1	whatever					714:721	whatever	714:721	whatever	714:721	DSC and XRD results showed that starch was amorphous whatever extrusion conditions.					
29735050	7	87	theme	determining	1068:1078	arg1	temperatures					1086:1097	determining onset temperatures	1068:1097	determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content	1068:1188	These trends could be described by sigmoid models, which allowed determining onset temperatures for changes of protein solubility in the interval [125, 146 °C], whatever moisture content.					
29735050	2	88	theme	different	380:388	arg1	scales					390:395	different scales	380:395	different scales	380:395	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	89	from	solubility	510:519	arg1	solubility					542:551	protein solubility	534:551	protein solubility	534:551	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	89	from	solubility	510:519	arg1	water					524:528	water	524:528	water	524:528	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	4	90	theme	DSC	661:663	arg1	results					673:679	DSC and XRD results	661:679	DSC and XRD results	661:679	DSC and XRD results showed that starch was amorphous whatever extrusion conditions.					
29735050	6	91	from	soluble	933:939	arg1	DTE					944:946	DTE	944:946	DTE	944:946	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	1	92	theme	product	232:238	arg1	contents					205:212	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	92	theme	product	232:238	arg1	115-165 °C					255:264	115-165 °C	255:264	115-165 °C	255:264	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	92	theme	product	232:238	arg1	T					252:252	product temperature T	232:252	product temperature T (115-165 °C)	232:265	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	2	93	from	water	524:528	arg1	SE-HPLC					569:575	SE-HPLC	569:575	SE-HPLC	569:575	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	93	from	water	524:528	arg1	DTE					564:566	DTE	564:566	DTE (SE-HPLC)	564:576	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	2	93	from	water	524:528	arg1	SDS					556:558	SDS	556:558	SDS	556:558	Structural changes of extruded pea flour were determined at different scales by measurements of density (expansion), crystallinity (X-ray diffraction), gelatinisation enthalpy (DSC), starch solubility in water and protein solubility in SDS and DTE (SE-HPLC).					
29735050	10	94	theme	amorphous	1656:1664	arg1	starch					1666:1671	amorphous starch	1656:1671	amorphous starch	1656:1671	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	4	95	theme	XRD	669:671	arg1	results					673:679	DSC and XRD results	661:679	DSC and XRD results	661:679	DSC and XRD results showed that starch was amorphous whatever extrusion conditions.					
29735050	6	96	from	DTE	944:946	arg1	soluble					933:939	soluble	933:939	soluble	933:939	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	8	97	theme	similar	1237:1243	arg1	trends					1245:1250	similar trends	1237:1250	similar trends	1237:1250	The SME impact on protein solubility followed similar trends.					
29735050	6	98	theme	soluble	933:939	arg1	proteins					924:931	the proteins	920:931	the proteins soluble in DTE	920:946	Increasing temperature from 115 to 165 °C decreased proteins soluble in SDS from 95 to 35% (R2 = 0.83) of total proteins, whereas the proteins soluble in DTE increased from 5 to 45% (R2 = 0.75) of total proteins.					
29735050	10	99	theme	aggregates	1641:1650	arg1	matrix					1617:1622	a bi-continuous matrix	1601:1622	a bi-continuous matrix of large protein aggregates and amorphous starch	1601:1671	CSLM images suggested that extruded pea flour had a composite morphology that changed from dispersed small protein aggregates to a bi-continuous matrix of large protein aggregates and amorphous starch.					
29735050	1	100	theme	various	188:194	arg1	contents					205:212	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg)	188:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	100	theme	various	188:194	arg1	SME					299:301	specific mechanical energy SME	272:301	specific mechanical energy SME (50-1200 kJ/kg)	272:317	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	100	theme	various	188:194	arg1	MC					214:215	MC	214:215	MC (18-35% w.b.)	214:229	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29735050	1	100	theme	various	188:194	arg1	T					252:252	product temperature T	232:252	product temperature T (115-165 °C)	232:265	Dehulled yellow pea flour (48.2% starch, 23.4% proteins, d.b.), was processed by a twin-screw extruder at various moisture contents MC (18-35% w.b.), product temperature T (115-165 °C), and specific mechanical energy SME (50-1200 kJ/kg).					
29034837	11	0	theme	remnant	1431:1437	arg1	cells					1449:1453	remnant cancerous cells	1431:1453	remnant cancerous cells	1431:1453	An injection of dox-alg composite gel in the tissue would fill the void created after tumor removal surgery, prevent the resuscitation of remnant cancerous cells and reduce inflammation.					
29034837	12	1	theme	composite	1509:1517	arg1	agent					1544:1548	a potential agent	1532:1548	a potential agent for the dual anti-cancer and anti-inflammatory therapy	1532:1603	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	12	1	theme	composite	1509:1517	arg1	gel					1519:1521	the dox-alg composite gel	1497:1521	the dox-alg composite gel	1497:1521	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	7	2	theme	release	1024:1030	arg1	day					1037:1039	sustained release till day 21	1014:1042	sustained release till day 21	1014:1042	Burst release was observed for both drugs within 24 h followed by sustained release till day 21.					
29034837	11	3	theme	cancerous	1439:1447	arg1	cells					1449:1453	remnant cancerous cells	1431:1453	remnant cancerous cells	1431:1453	An injection of dox-alg composite gel in the tissue would fill the void created after tumor removal surgery, prevent the resuscitation of remnant cancerous cells and reduce inflammation.					
29034837	0	4	theme	Anti-Cancer	107:117	arg1	Therapy					141:147	Anti-Cancer and Anti-Inflammatory Therapy	107:147	Anti-Cancer and Anti-Inflammatory Therapy	107:147	Dual-purpose Injectable Doxorubicin Conjugated Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy.					
29034837	2	5	theme	in	394:395	arg1	system					406:411	in situ gel system	394:411	in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity	394:509	OBJECTIVE Herein, in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity is reported.					
29034837	10	6	theme	free	1282:1285	arg1	drug					1287:1290	the free drug	1278:1290	the free drug	1278:1290	Anti-inflammatory activity of ibuprofen released from composite gel was compared with the free drug.					
29034837	8	7	theme	considerable	1088:1099	arg1	effect					1111:1116	considerable cytotoxic effect	1088:1116	considerable cytotoxic effect	1088:1116	Doxorubicin released from composite showed considerable cytotoxic effect.					
29034837	9	8	theme	Cell	1119:1122	arg1	uptake					1124:1129	Cell uptake	1119:1129	Cell uptake	1119:1129	Cell uptake was confirmed by confocal microscopy using MDA-MB-231 cells.					
29034837	1	9	theme	targeted	298:305	arg1	delivery					312:319	targeted drug delivery	298:319	targeted drug delivery	298:319	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	0	10	theme	Anti-Inflammatory	123:139	arg1	Therapy					141:147	Anti-Cancer and Anti-Inflammatory Therapy	107:147	Anti-Cancer and Anti-Inflammatory Therapy	107:147	Dual-purpose Injectable Doxorubicin Conjugated Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy.					
29034837	12	11	theme	anti-cancer	1563:1573	arg1	therapy					1597:1603	the dual anti-cancer and anti-inflammatory therapy	1554:1603	the dual anti-cancer and anti-inflammatory therapy	1554:1603	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	2	12	dep	in	394:395	arg1	situ					397:400	situ	397:400	situ	397:400	OBJECTIVE Herein, in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity is reported.					
29034837	1	13	theme	drug	307:310	arg1	delivery					312:319	targeted drug delivery	298:319	targeted drug delivery	298:319	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	7	14	located	observed	966:973	arg1	h					1000:1000	24 h	997:1000	24 h followed by sustained release till day 21	997:1042	Burst release was observed for both drugs within 24 h followed by sustained release till day 21.					
29034837	7	14	located	observed	966:973	arg2	release					954:960	Burst release	948:960	Burst release	948:960	Burst release was observed for both drugs within 24 h followed by sustained release till day 21.					
29034837	2	15	theme	ibuprofen	454:462	arg1	release					427:433	sustained release	417:433	sustained release of doxorubicin and ibuprofen	417:462	OBJECTIVE Herein, in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity is reported.					
29034837	1	16	dep	In	161:162	arg1	situ					164:167	situ	164:167	situ	164:167	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	12	17	theme	potential	1534:1542	arg1	agent					1544:1548	a potential agent	1532:1548	a potential agent for the dual anti-cancer and anti-inflammatory therapy	1532:1603	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	12	17	theme	potential	1534:1542	arg1	gel					1519:1521	the dox-alg composite gel	1497:1521	the dox-alg composite gel	1497:1521	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	8	18	attach	released	1057:1064	arg2	Doxorubicin					1045:1055	Doxorubicin	1045:1055	Doxorubicin released from composite	1045:1079	Doxorubicin released from composite showed considerable cytotoxic effect.					
29034837	8	18	attach	released	1057:1064	arg1	composite					1071:1079	composite	1071:1079	composite	1071:1079	Doxorubicin released from composite showed considerable cytotoxic effect.					
29034837	6	19	theme	formation	926:934	arg1	properties					936:945	good syringeability and gel formation properties	898:945	good syringeability and gel formation properties	898:945	RESULTS Dox-alg composite gel showed good syringeability and gel formation properties.					
29034837	8	20	theme	cytotoxic	1101:1109	arg1	effect					1111:1116	considerable cytotoxic effect	1088:1116	considerable cytotoxic effect	1088:1116	Doxorubicin released from composite showed considerable cytotoxic effect.					
29034837	2	21	theme	anti-inflammatory	484:500	arg1	activity					502:509	anti-cancer and anti-inflammatory activity	468:509	anti-cancer and anti-inflammatory activity	468:509	OBJECTIVE Herein, in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity is reported.					
29034837	10	22	theme	ibuprofen	1222:1230	arg1	activity					1210:1217	Anti-inflammatory activity	1192:1217	Anti-inflammatory activity of ibuprofen released from composite gel	1192:1258	Anti-inflammatory activity of ibuprofen released from composite gel was compared with the free drug.					
29034837	0	23	theme	Injectable	13:22	arg1	Doxorubicin					24:34	Dual-purpose Injectable Doxorubicin	0:34	Dual-purpose Injectable Doxorubicin	0:34	Dual-purpose Injectable Doxorubicin Conjugated Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy.					
29034837	6	24	theme	gel	922:924	arg1	formation					926:934	gel formation	922:934	gel formation	922:934	RESULTS Dox-alg composite gel showed good syringeability and gel formation properties.					
29034837	2	25	dep	OBJECTIVE	376:384	arg1	reported					514:521	reported	514:521	is reported	511:521	OBJECTIVE Herein, in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity is reported.					
29034837	2	26	theme	sustained	417:425	arg1	release					427:433	sustained release	417:433	sustained release of doxorubicin and ibuprofen	417:462	OBJECTIVE Herein, in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity is reported.					
29034837	0	27	theme	Dual-purpose	0:11	arg1	Doxorubicin					24:34	Dual-purpose Injectable Doxorubicin	0:34	Dual-purpose Injectable Doxorubicin	0:34	Dual-purpose Injectable Doxorubicin Conjugated Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy.					
29034837	3	28	theme	encapsulated	640:651	arg1	microparticles					676:689	ibuprofen encapsulated polycaprolactone (PCL) microparticles	630:689	ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite)	630:709	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	3	28	theme	encapsulated	640:651	arg1	composite					700:708	dox-alg composite	692:708	dox-alg composite	692:708	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	9	29	theme	confocal	1148:1155	arg1	microscopy					1157:1166	confocal microscopy	1148:1166	confocal microscopy using MDA-MB-231 cells	1148:1189	Cell uptake was confirmed by confocal microscopy using MDA-MB-231 cells.					
29034837	3	30	theme	METHOD	524:529	arg1	gel					573:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel	524:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel	524:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	5	31	theme	TGA	847:849	arg1	analysis					851:858	TGA analysis	847:858	TGA analysis	847:858	The gel was characterized using SEM, FTIR, XRD and TGA analysis.					
29034837	1	32	theme	sustained	229:237	arg1	systems					256:262	sustained release delivery systems	229:262	sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity	229:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	2	33	theme	anti-cancer	468:478	arg1	activity					502:509	anti-cancer and anti-inflammatory activity	468:509	anti-cancer and anti-inflammatory activity	468:509	OBJECTIVE Herein, in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity is reported.					
29034837	1	34	theme	minimal	322:328	arg1	invasiveness					330:341	minimal invasiveness	322:341	minimal invasiveness	322:341	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	12	35	dep	CONCLUSION	1480:1489	arg1	agent					1544:1548	a potential agent	1532:1548	a potential agent for the dual anti-cancer and anti-inflammatory therapy	1532:1603	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	12	35	dep	CONCLUSION	1480:1489	arg1	gel					1519:1521	the dox-alg composite gel	1497:1521	the dox-alg composite gel	1497:1521	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	12	36	theme	dual	1558:1561	arg1	therapy					1597:1603	the dual anti-cancer and anti-inflammatory therapy	1554:1603	the dual anti-cancer and anti-inflammatory therapy	1554:1603	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	1	37	theme	release	239:245	arg1	systems					256:262	sustained release delivery systems	229:262	sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity	229:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	0	38	theme	Gel	56:58	arg1	Microparticles					88:101	Alginate Gel Containing Polycaprolactone Microparticles	47:101	Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy	47:147	Dual-purpose Injectable Doxorubicin Conjugated Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy.					
29034837	4	39	theme	size	778:781	arg1	method					770:775	a solvent evaporation method	748:775	a solvent evaporation method (size 50 - 100µm)	748:793	PCL microparticles were prepared by a solvent evaporation method (size 50 - 100µm).					
29034837	4	39	theme	size	778:781	arg1	100µm					788:792	size 50 - 100µm	778:792	size 50 - 100µm	778:792	PCL microparticles were prepared by a solvent evaporation method (size 50 - 100µm).					
29034837	6	40	theme	syringeability	903:916	arg1	properties					936:945	good syringeability and gel formation properties	898:945	good syringeability and gel formation properties	898:945	RESULTS Dox-alg composite gel showed good syringeability and gel formation properties.					
29034837	3	41	theme	PCL	671:673	arg1	microparticles					676:689	ibuprofen encapsulated polycaprolactone (PCL) microparticles	630:689	ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite)	630:709	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	3	41	theme	PCL	671:673	arg1	composite					700:708	dox-alg composite	692:708	dox-alg composite	692:708	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	9	42	theme	MDA-MB-231	1174:1183	arg1	cells					1185:1189	MDA-MB-231 cells	1174:1189	MDA-MB-231 cells	1174:1189	Cell uptake was confirmed by confocal microscopy using MDA-MB-231 cells.					
29034837	1	43	theme	delivery	247:254	arg1	systems					256:262	sustained release delivery systems	229:262	sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity	229:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	0	44	theme	Alginate	47:54	arg1	Microparticles					88:101	Alginate Gel Containing Polycaprolactone Microparticles	47:101	Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy	47:147	Dual-purpose Injectable Doxorubicin Conjugated Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy.					
29034837	12	45	theme	anti-inflammatory	1579:1595	arg1	therapy					1597:1603	the dual anti-cancer and anti-inflammatory therapy	1554:1603	the dual anti-cancer and anti-inflammatory therapy	1554:1603	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	6	46	theme	good	898:901	arg1	properties					936:945	good syringeability and gel formation properties	898:945	good syringeability and gel formation properties	898:945	RESULTS Dox-alg composite gel showed good syringeability and gel formation properties.					
29034837	11	47	theme	tumor	1379:1383	arg1	surgery					1393:1399	tumor removal surgery	1379:1399	tumor removal surgery	1379:1399	An injection of dox-alg composite gel in the tissue would fill the void created after tumor removal surgery, prevent the resuscitation of remnant cancerous cells and reduce inflammation.					
29034837	10	48	attach	released	1232:1239	arg2	ibuprofen					1222:1230	ibuprofen	1222:1230	ibuprofen released from composite gel	1222:1258	Anti-inflammatory activity of ibuprofen released from composite gel was compared with the free drug.					
29034837	10	48	attach	released	1232:1239	arg1	gel					1256:1258	composite gel	1246:1258	composite gel	1246:1258	Anti-inflammatory activity of ibuprofen released from composite gel was compared with the free drug.					
29034837	2	49	theme	doxorubicin	438:448	arg1	release					427:433	sustained release	417:433	sustained release of doxorubicin and ibuprofen	417:462	OBJECTIVE Herein, in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity is reported.					
29034837	0	50	theme	Polycaprolactone	71:86	arg1	Microparticles					88:101	Alginate Gel Containing Polycaprolactone Microparticles	47:101	Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy	47:147	Dual-purpose Injectable Doxorubicin Conjugated Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy.					
29034837	1	51	theme	potent	347:352	arg1	activity					366:373	potent therapeutic activity	347:373	potent therapeutic activity	347:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	11	52	theme	gel	1327:1329	arg1	injection					1296:1304	An injection	1293:1304	An injection of dox-alg composite gel in the tissue	1293:1343	An injection of dox-alg composite gel in the tissue would fill the void created after tumor removal surgery, prevent the resuscitation of remnant cancerous cells and reduce inflammation.					
29034837	10	53	theme	Anti-inflammatory	1192:1208	arg1	activity					1210:1217	Anti-inflammatory activity	1192:1217	Anti-inflammatory activity of ibuprofen released from composite gel	1192:1258	Anti-inflammatory activity of ibuprofen released from composite gel was compared with the free drug.					
29034837	3	54	theme	polycaprolactone	653:668	arg1	microparticles					676:689	ibuprofen encapsulated polycaprolactone (PCL) microparticles	630:689	ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite)	630:709	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	3	54	theme	polycaprolactone	653:668	arg1	composite					700:708	dox-alg composite	692:708	dox-alg composite	692:708	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	12	55	theme	dox-alg	1501:1507	arg1	agent					1544:1548	a potential agent	1532:1548	a potential agent for the dual anti-cancer and anti-inflammatory therapy	1532:1603	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	12	55	theme	dox-alg	1501:1507	arg1	gel					1519:1521	the dox-alg composite gel	1497:1521	the dox-alg composite gel	1497:1521	CONCLUSION Thus, the dox-alg composite gel could be a potential agent for the dual anti-cancer and anti-inflammatory therapy.					
29034837	1	56	theme	In	161:162	arg1	carrier					217:223	a carrier	215:223	a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity	215:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	1	56	theme	In	161:162	arg1	formulations					173:184	In situ gel formulations	161:184	In situ gel formulations	161:184	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	0	57	theme	Containing	60:69	arg1	Microparticles					88:101	Alginate Gel Containing Polycaprolactone Microparticles	47:101	Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy	47:147	Dual-purpose Injectable Doxorubicin Conjugated Alginate Gel Containing Polycaprolactone Microparticles for Anti-Cancer and Anti-Inflammatory Therapy.					
29034837	1	58	theme	therapeutic	354:364	arg1	activity					366:373	potent therapeutic activity	347:373	potent therapeutic activity	347:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	11	59	theme	composite	1317:1325	arg1	gel					1327:1329	dox-alg composite gel	1309:1329	dox-alg composite gel	1309:1329	An injection of dox-alg composite gel in the tissue would fill the void created after tumor removal surgery, prevent the resuscitation of remnant cancerous cells and reduce inflammation.					
29034837	3	60	theme	dox-alg	564:570	arg1	gel					573:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel	524:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel	524:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	3	61	theme	ibuprofen	630:638	arg1	microparticles					676:689	ibuprofen encapsulated polycaprolactone (PCL) microparticles	630:689	ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite)	630:709	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	3	61	theme	ibuprofen	630:638	arg1	composite					700:708	dox-alg composite	692:708	dox-alg composite	692:708	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	11	62	theme	dox-alg	1309:1315	arg1	gel					1327:1329	dox-alg composite gel	1309:1329	dox-alg composite gel	1309:1329	An injection of dox-alg composite gel in the tissue would fill the void created after tumor removal surgery, prevent the resuscitation of remnant cancerous cells and reduce inflammation.					
29034837	6	63	theme	composite	877:885	arg1	gel					887:889	composite gel	877:889	composite gel	877:889	RESULTS Dox-alg composite gel showed good syringeability and gel formation properties.					
29034837	4	64	theme	solvent	750:756	arg1	method					770:775	a solvent evaporation method	748:775	a solvent evaporation method (size 50 - 100µm)	748:793	PCL microparticles were prepared by a solvent evaporation method (size 50 - 100µm).					
29034837	4	64	theme	solvent	750:756	arg1	100µm					788:792	size 50 - 100µm	778:792	size 50 - 100µm	778:792	PCL microparticles were prepared by a solvent evaporation method (size 50 - 100µm).					
29034837	1	65	theme	gel	169:171	arg1	carrier					217:223	a carrier	215:223	a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity	215:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	1	65	theme	gel	169:171	arg1	formulations					173:184	In situ gel formulations	161:184	In situ gel formulations	161:184	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	1	66	theme	due	264:266	arg1	systems					256:262	sustained release delivery systems	229:262	sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity	229:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	3	67	theme	EDC-NHS	596:602	arg1	chemistry					604:612	EDC-NHS chemistry	596:612	EDC-NHS chemistry	596:612	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	7	68	theme	till	1032:1035	arg1	day					1037:1039	sustained release till day 21	1014:1042	sustained release till day 21	1014:1042	Burst release was observed for both drugs within 24 h followed by sustained release till day 21.					
29034837	2	69	theme	gel	402:404	arg1	system					406:411	in situ gel system	394:411	in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity	394:509	OBJECTIVE Herein, in situ gel system for sustained release of doxorubicin and ibuprofen for anti-cancer and anti-inflammatory activity is reported.					
29034837	7	70	theme	Burst	948:952	arg1	release					954:960	Burst release	948:960	Burst release	948:960	Burst release was observed for both drugs within 24 h followed by sustained release till day 21.					
29034837	3	71	theme	Doxorubicin-conjugated	531:552	arg1	gel					573:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel	524:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel	524:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	11	72	theme	cells	1449:1453	arg1	resuscitation					1414:1426	the resuscitation	1410:1426	the resuscitation of remnant cancerous cells	1410:1453	An injection of dox-alg composite gel in the tissue would fill the void created after tumor removal surgery, prevent the resuscitation of remnant cancerous cells and reduce inflammation.					
29034837	4	73	theme	PCL	712:714	arg1	microparticles					716:729	PCL microparticles	712:729	PCL microparticles	712:729	PCL microparticles were prepared by a solvent evaporation method (size 50 - 100µm).					
29034837	4	74	theme	evaporation	758:768	arg1	method					770:775	a solvent evaporation method	748:775	a solvent evaporation method (size 50 - 100µm)	748:793	PCL microparticles were prepared by a solvent evaporation method (size 50 - 100µm).					
29034837	4	74	theme	evaporation	758:768	arg1	100µm					788:792	size 50 - 100µm	778:792	size 50 - 100µm	778:792	PCL microparticles were prepared by a solvent evaporation method (size 50 - 100µm).					
29034837	3	75	theme	alginate	554:561	arg1	gel					573:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel	524:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel	524:575	METHOD Doxorubicin-conjugated alginate (dox-alg) gel was prepared using EDC-NHS chemistry and loaded with ibuprofen encapsulated polycaprolactone (PCL) microparticles (dox-alg composite).					
29034837	1	76	theme	certain	271:277	arg1	delivery					312:319	targeted drug delivery	298:319	targeted drug delivery	298:319	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	1	76	theme	certain	271:277	arg1	advantages					279:288	certain advantages	271:288	certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity	271:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	1	76	theme	certain	271:277	arg1	invasiveness					330:341	minimal invasiveness	322:341	minimal invasiveness	322:341	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	1	76	theme	certain	271:277	arg1	activity					366:373	potent therapeutic activity	347:373	potent therapeutic activity	347:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	11	77	from	injection	1296:1304	arg1	tissue					1338:1343	the tissue	1334:1343	the tissue	1334:1343	An injection of dox-alg composite gel in the tissue would fill the void created after tumor removal surgery, prevent the resuscitation of remnant cancerous cells and reduce inflammation.					
29034837	11	78	theme	removal	1385:1391	arg1	surgery					1393:1399	tumor removal surgery	1379:1399	tumor removal surgery	1379:1399	An injection of dox-alg composite gel in the tissue would fill the void created after tumor removal surgery, prevent the resuscitation of remnant cancerous cells and reduce inflammation.					
29034837	7	79	theme	sustained	1014:1022	arg1	day					1037:1039	sustained release till day 21	1014:1042	sustained release till day 21	1014:1042	Burst release was observed for both drugs within 24 h followed by sustained release till day 21.					
29034837	1	80	dep	BACKGROUND	150:159	arg1	reported					203:210	reported	203:210	have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity	186:373	BACKGROUND In situ gel formulations have been widely reported as a carrier for sustained release delivery systems due to certain advantages such as targeted drug delivery, minimal invasiveness and potent therapeutic activity.					
29034837	10	81	theme	composite	1246:1254	arg1	gel					1256:1258	composite gel	1246:1258	composite gel	1246:1258	Anti-inflammatory activity of ibuprofen released from composite gel was compared with the free drug.					
31547444	9	0	used	used	1601:1604	arg2	construct					1587:1595	the cell/cryogel construct	1570:1595	the cell/cryogel construct	1570:1595	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	7	1	from	change	1250:1255	arg1	cytoskeleton					1284:1295	cytoskeleton	1284:1295	cytoskeleton	1284:1295	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	7	1	from	change	1250:1255	arg1	morphology					1269:1278	the cell morphology	1260:1278	the cell morphology	1260:1278	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	7	2	theme	expression	1401:1410	arg1	rate					1337:1340	reduced cell proliferation rate	1310:1340	reduced cell proliferation rate	1310:1340	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	7	2	theme	expression	1401:1410	arg1	downregulation					1347:1360	downregulation	1347:1360	downregulation of the mesothelium specific maker gene expression	1347:1410	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	7	2	theme	expression	1401:1410	arg1	change					1250:1255	a change	1248:1255	a change in the cell morphology and cytoskeleton arrangement	1248:1307	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	4	3	theme	scaffold	842:849	arg1	degradation					851:861	a faster scaffold degradation	833:861	a faster scaffold degradation in the collagenase solution	833:889	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	6	4	theme	cell	1126:1129	arg1	viability					1131:1139	quantitative cell viability	1113:1139	quantitative cell viability	1113:1139	From the in vitro cell culture, rat mesothelial cells showed quantitative cell viability in gelatin (G) and gelatin/HA (GH) cryogels.					
31547444	0	5	dep	Culture	80:86	arg1	Regeneration					132:143	Tissue Regeneration	125:143	Tissue Regeneration	125:143	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	7	6	theme	maker	1390:1394	arg1	expression					1401:1410	the mesothelium specific maker gene expression	1365:1410	the mesothelium specific maker gene expression	1365:1410	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	10	7	theme	immunohistochemical	1848:1866	arg1	staining					1868:1875	immunohistochemical staining	1848:1875	immunohistochemical staining	1848:1875	A mesothelium layer similar to the native mesothelium tissue could be obtained 21 days post-implantation, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
31547444	6	8	theme	mesothelial	1088:1098	arg1	cells					1100:1104	rat mesothelial cells	1084:1104	rat mesothelial cells	1084:1104	From the in vitro cell culture, rat mesothelial cells showed quantitative cell viability in gelatin (G) and gelatin/HA (GH) cryogels.					
31547444	2	9	theme	culture	310:316	arg1	cells					330:334	culture mesothelial cells	310:334	culture mesothelial cells	310:334	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a three-dimensional (3D) scaffold for tissue engineering applications.					
31547444	7	10	theme	reduced	1310:1316	arg1	rate					1337:1340	reduced cell proliferation rate	1310:1340	reduced cell proliferation rate	1310:1340	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	9	11	dep	in	1553:1554	arg1	vivo					1556:1559	vivo	1556:1559	vivo	1556:1559	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	3	12	theme	HA	559:560	arg1	influence					546:554	the influence	542:554	the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds	542:663	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	3	13	theme	cellular	592:599	arg1	phenotype					601:609	the cellular phenotype	588:609	the cellular phenotype	588:609	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	7	14	dep	morphology	1269:1278	arg1	arrangement					1297:1307	arrangement	1297:1307	arrangement	1297:1307	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	9	15	theme	mesothelial	1643:1653	arg1	cells					1655:1659	allograft mesothelial cells	1633:1659	allograft mesothelial cells	1633:1659	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	4	16	theme	collagenase	870:880	arg1	solution					882:889	the collagenase solution	866:889	the collagenase solution	866:889	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	2	17	theme	few	265:267	arg1	studies					269:275	few studies	265:275	few studies	265:275	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a three-dimensional (3D) scaffold for tissue engineering applications.					
31547444	7	18	theme	cell	1264:1267	arg1	morphology					1269:1278	the cell morphology	1260:1278	the cell morphology	1260:1278	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	3	19	theme	cells	626:630	arg1	properties					573:582	cryogel properties	565:582	cryogel properties	565:582	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	3	19	theme	cells	626:630	arg1	phenotype					601:609	the cellular phenotype	588:609	the cellular phenotype	588:609	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	5	20	theme	5	899:899	arg1	%					900:900	%	900:900	%	900:900	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	4	21	theme	HA	687:688	arg1	incorporation					670:682	The incorporation	666:682	The incorporation of HA	666:688	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	0	22	theme	3D	77:78	arg1	Culture					80:86	the 3D Culture	73:86	the 3D Culture of Mesothelial Cells	73:107	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	1	23	theme	specific	168:175	arg1	cells					158:162	Mesothelial cells	146:162	Mesothelial cells	146:162	Mesothelial cells are specific epithelial cells that are lined in the serosal cavity and internal organs.					
31547444	1	23	theme	specific	168:175	arg1	cells					188:192	specific epithelial cells	168:192	specific epithelial cells that are lined in the serosal cavity and internal organs	168:249	Mesothelial cells are specific epithelial cells that are lined in the serosal cavity and internal organs.					
31547444	7	24	from	downregulation	1347:1360	arg1	cytoskeleton					1284:1295	cytoskeleton	1284:1295	cytoskeleton	1284:1295	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	7	24	from	downregulation	1347:1360	arg1	morphology					1269:1278	the cell morphology	1260:1278	the cell morphology	1260:1278	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	8	25	theme	key	1431:1433	arg1	E-cadherin					1456:1465	E-cadherin	1456:1465	E-cadherin	1456:1465	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	8	25	theme	key	1431:1433	arg1	proteins					1447:1454	key mesothelium proteins E-cadherin and calretinin	1431:1480	key mesothelium proteins E-cadherin and calretinin	1431:1480	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	8	25	theme	key	1431:1433	arg1	calretinin					1471:1480	calretinin	1471:1480	calretinin	1471:1480	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	3	26	from	influence	546:554	arg1	properties					573:582	cryogel properties	565:582	cryogel properties	565:582	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	3	26	from	influence	546:554	arg1	phenotype					601:609	the cellular phenotype	588:609	the cellular phenotype	588:609	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	5	27	theme	elastic	1035:1041	arg1	modulus					1043:1049	the elastic modulus	1031:1049	the elastic modulus	1031:1049	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	8	28	theme	proteins	1447:1454	arg1	production					1417:1426	The production	1413:1426	The production of key mesothelium proteins E-cadherin and calretinin	1413:1480	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	4	29	theme	uptake	759:764	arg1	kinetics					766:773	water uptake kinetics	753:773	water uptake kinetics	753:773	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	0	30	theme	Cells	103:107	arg1	Mesothelium					113:123	Mesothelium	113:123	Mesothelium	113:123	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	0	30	theme	Cells	103:107	arg1	Culture					80:86	the 3D Culture	73:86	the 3D Culture of Mesothelial Cells	73:107	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	4	31	theme	pore	732:735	arg1	size					737:740	pore size	732:740	pore size	732:740	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	2	32	theme	tissue	377:382	arg1	applications					396:407	tissue engineering applications	377:407	tissue engineering applications	377:407	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a three-dimensional (3D) scaffold for tissue engineering applications.					
31547444	4	33	from	degradation	851:861	arg1	solution					882:889	the collagenase solution	866:889	the collagenase solution	866:889	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	2	34	dep	three-dimensional	341:357	arg1	3D					360:361	3D	360:361	3D	360:361	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a three-dimensional (3D) scaffold for tissue engineering applications.					
31547444	0	35	theme	Gelatin	15:21	arg1	Preparation					0:10	Preparation	0:10	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel	0:57	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	7	36	theme	GH	1229:1230	arg1	cryogels					1232:1239	GH cryogels	1229:1239	GH cryogels	1229:1239	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	3	37	theme	3D	652:653	arg1	scaffolds					655:663	the 3D scaffolds	648:663	the 3D scaffolds	648:663	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	0	38	theme	Gelatin/Hyaluronic	27:44	arg1	Cryogel					51:57	Gelatin/Hyaluronic Acid Cryogel	27:57	Gelatin/Hyaluronic Acid Cryogel	27:57	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	4	39	theme	cryogel	803:809	arg1	scaffolds					811:819	the cryogel scaffolds	799:819	the cryogel scaffolds	799:819	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	2	40	theme	three-dimensional	341:357	arg1	scaffold					364:371	a three-dimensional (3D) scaffold	339:371	a three-dimensional (3D) scaffold for tissue engineering applications	339:407	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a three-dimensional (3D) scaffold for tissue engineering applications.					
31547444	0	41	theme	Cryogel	51:57	arg1	Preparation					0:10	Preparation	0:10	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel	0:57	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	9	42	theme	G	1538:1538	arg1	cryogels					1540:1547	the best G cryogels	1529:1547	the best G cryogels for in vivo studies	1529:1567	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	9	43	theme	cell/cryogel	1574:1585	arg1	construct					1587:1595	the cell/cryogel construct	1570:1595	the cell/cryogel construct	1570:1595	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	7	44	theme	mesothelial	1199:1209	arg1	cells					1211:1215	mesothelial cells	1199:1215	mesothelial cells cultured in GH cryogels	1199:1239	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	6	45	dep	in	1061:1062	arg1	vitro					1064:1068	vitro	1064:1068	vitro	1064:1068	From the in vitro cell culture, rat mesothelial cells showed quantitative cell viability in gelatin (G) and gelatin/HA (GH) cryogels.					
31547444	3	46	theme	gelatin/hyaluronic	481:498	arg1	HA					506:507	HA	506:507	HA	506:507	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	3	46	theme	gelatin/hyaluronic	481:498	arg1	acid					500:503	gelatin/hyaluronic acid	481:503	gelatin/hyaluronic acid (HA)	481:508	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	9	47	from	reconstruction	1677:1690	arg1	rats					1695:1698	rats	1695:1698	rats	1695:1698	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	5	48	theme	ultimate	950:957	arg1	strain					979:984	strain	979:984	strain	979:984	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	5	48	theme	ultimate	950:957	arg1	stress					971:976	the ultimate compressive stress	946:976	the ultimate compressive stress (strain)	946:985	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	4	49	theme	swelling	780:787	arg1	ratios					789:794	the pore size, porosity, water uptake kinetics, and swelling ratios	728:794	the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds	728:819	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	1	50	theme	internal	235:242	arg1	organs					244:249	internal organs	235:249	internal organs	235:249	Mesothelial cells are specific epithelial cells that are lined in the serosal cavity and internal organs.					
31547444	7	51	theme	gene	1396:1399	arg1	expression					1401:1410	the mesothelium specific maker gene expression	1365:1410	the mesothelium specific maker gene expression	1365:1410	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	7	52	theme	proliferation	1323:1335	arg1	rate					1337:1340	reduced cell proliferation rate	1310:1340	reduced cell proliferation rate	1310:1340	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	7	53	theme	specific	1381:1388	arg1	expression					1401:1410	the mesothelium specific maker gene expression	1365:1410	the mesothelium specific maker gene expression	1365:1410	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	4	54	theme	faster	835:840	arg1	degradation					851:861	a faster scaffold degradation	833:861	a faster scaffold degradation in the collagenase solution	833:889	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	6	55	theme	quantitative	1113:1124	arg1	viability					1131:1139	quantitative cell viability	1113:1139	quantitative cell viability	1113:1139	From the in vitro cell culture, rat mesothelial cells showed quantitative cell viability in gelatin (G) and gelatin/HA (GH) cryogels.					
31547444	1	56	theme	epithelial	177:186	arg1	cells					158:162	Mesothelial cells	146:162	Mesothelial cells	146:162	Mesothelial cells are specific epithelial cells that are lined in the serosal cavity and internal organs.					
31547444	1	56	theme	epithelial	177:186	arg1	cells					188:192	specific epithelial cells	168:192	specific epithelial cells that are lined in the serosal cavity and internal organs	168:249	Mesothelial cells are specific epithelial cells that are lined in the serosal cavity and internal organs.					
31547444	4	57	theme	scaffolds	811:819	arg1	ratios					789:794	the pore size, porosity, water uptake kinetics, and swelling ratios	728:794	the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds	728:819	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	2	58	theme	mesothelial	318:328	arg1	cells					330:334	culture mesothelial cells	310:334	culture mesothelial cells	310:334	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a three-dimensional (3D) scaffold for tissue engineering applications.					
31547444	6	59	theme	rat	1084:1086	arg1	cells					1100:1104	rat mesothelial cells	1084:1104	rat mesothelial cells	1084:1104	From the in vitro cell culture, rat mesothelial cells showed quantitative cell viability in gelatin (G) and gelatin/HA (GH) cryogels.					
31547444	0	60	theme	Tissue	125:130	arg1	Regeneration					132:143	Tissue Regeneration	125:143	Tissue Regeneration	125:143	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	10	61	theme	mesothelium	1703:1713	arg1	layer					1715:1719	A mesothelium layer	1701:1719	A mesothelium layer similar to the native mesothelium tissue	1701:1760	A mesothelium layer similar to the native mesothelium tissue could be obtained 21 days post-implantation, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
31547444	3	62	theme	cryogel	565:571	arg1	properties					573:582	cryogel properties	565:582	cryogel properties	565:582	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	7	63	theme	cell	1318:1321	arg1	rate					1337:1340	reduced cell proliferation rate	1310:1340	reduced cell proliferation rate	1310:1340	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	10	64	theme	mesothelium	1743:1753	arg1	tissue					1755:1760	the native mesothelium tissue	1732:1760	the native mesothelium tissue	1732:1760	A mesothelium layer similar to the native mesothelium tissue could be obtained 21 days post-implantation, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
31547444	9	65	theme	allograft	1633:1641	arg1	cells					1655:1659	allograft mesothelial cells	1633:1659	allograft mesothelial cells	1633:1659	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	1	66	dep	cavity	224:229	arg1	the					212:214	the	212:214	the	212:214	Mesothelial cells are specific epithelial cells that are lined in the serosal cavity and internal organs.					
31547444	9	67	theme	cells	1655:1659	arg1	transplantation					1614:1628	the transplantation	1610:1628	the transplantation of allograft mesothelial cells	1610:1659	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	3	68	theme	mesothelial	614:624	arg1	cells					626:630	mesothelial cells	614:630	mesothelial cells cultured within the 3D scaffolds	614:663	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	9	69	theme	mesothelium	1665:1675	arg1	reconstruction					1677:1690	mesothelium reconstruction	1665:1690	mesothelium reconstruction in rats	1665:1698	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	6	70	dep	gelatin	1144:1150	arg1	cryogels					1176:1183	cryogels	1176:1183	cryogels	1176:1183	From the in vitro cell culture, rat mesothelial cells showed quantitative cell viability in gelatin (G) and gelatin/HA (GH) cryogels.					
31547444	5	71	theme	scaffold	1008:1015	arg1	strain					979:984	strain	979:984	strain	979:984	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	5	71	theme	scaffold	1008:1015	arg1	toughness					991:999	toughness	991:999	toughness of the scaffold	991:1015	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	5	71	theme	scaffold	1008:1015	arg1	stress					971:976	the ultimate compressive stress	946:976	the ultimate compressive stress (strain)	946:985	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	1	72	theme	Mesothelial	146:156	arg1	cells					158:162	Mesothelial cells	146:162	Mesothelial cells	146:162	Mesothelial cells are specific epithelial cells that are lined in the serosal cavity and internal organs.					
31547444	1	72	theme	Mesothelial	146:156	arg1	cells					188:192	specific epithelial cells	168:192	specific epithelial cells that are lined in the serosal cavity and internal organs	168:249	Mesothelial cells are specific epithelial cells that are lined in the serosal cavity and internal organs.					
31547444	7	73	from	rate	1337:1340	arg1	cytoskeleton					1284:1295	cytoskeleton	1284:1295	cytoskeleton	1284:1295	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	7	73	from	rate	1337:1340	arg1	morphology					1269:1278	the cell morphology	1260:1278	the cell morphology	1260:1278	Nonetheless, mesothelial cells cultured in GH cryogels showed a change in the cell morphology and cytoskeleton arrangement, reduced cell proliferation rate, and downregulation of the mesothelium specific maker gene expression.					
31547444	8	74	theme	mesothelium	1435:1445	arg1	E-cadherin					1456:1465	E-cadherin	1456:1465	E-cadherin	1456:1465	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	8	74	theme	mesothelium	1435:1445	arg1	proteins					1447:1454	key mesothelium proteins E-cadherin and calretinin	1431:1480	key mesothelium proteins E-cadherin and calretinin	1431:1480	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	8	74	theme	mesothelium	1435:1445	arg1	calretinin					1471:1480	calretinin	1471:1480	calretinin	1471:1480	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	0	75	theme	Mesothelial	91:101	arg1	Cells					103:107	Mesothelial Cells	91:107	Mesothelial Cells	91:107	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	5	76	theme	composite	912:920	arg1	cryogels					922:929	the composite cryogels	908:929	the composite cryogels	908:929	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	10	77	theme	similar	1721:1727	arg1	layer					1715:1719	A mesothelium layer	1701:1719	A mesothelium layer similar to the native mesothelium tissue	1701:1760	A mesothelium layer similar to the native mesothelium tissue could be obtained 21 days post-implantation, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
31547444	4	78	theme	porosity	743:750	arg1	ratios					789:794	the pore size, porosity, water uptake kinetics, and swelling ratios	728:794	the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds	728:819	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	4	79	theme	water	753:757	arg1	kinetics					766:773	water uptake kinetics	753:773	water uptake kinetics	753:773	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	2	80	theme	engineering	384:394	arg1	applications					396:407	tissue engineering applications	377:407	tissue engineering applications	377:407	Nonetheless, few studies have explored the possibility to culture mesothelial cells in a three-dimensional (3D) scaffold for tissue engineering applications.					
31547444	5	81	theme	%	900:900	arg1	HA					902:903	5% HA	899:903	5% HA	899:903	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	9	82	theme	in	1553:1554	arg1	studies					1561:1567	in vivo studies	1553:1567	in vivo studies	1553:1567	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	4	83	theme	size	737:740	arg1	ratios					789:794	the pore size, porosity, water uptake kinetics, and swelling ratios	728:794	the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds	728:819	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	0	84	theme	Acid	46:49	arg1	Cryogel					51:57	Gelatin/Hyaluronic Acid Cryogel	27:57	Gelatin/Hyaluronic Acid Cryogel	27:57	Preparation of Gelatin and Gelatin/Hyaluronic Acid Cryogel Scaffolds for the 3D Culture of Mesothelial Cells and Mesothelium Tissue Regeneration.					
31547444	8	85	theme	GH	1507:1508	arg1	cryogels					1510:1517	the GH cryogels	1503:1517	the GH cryogels	1503:1517	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	10	86	theme	days	1783:1786	arg1	post-implantation					1788:1804	21 days post-implantation	1780:1804	21 days post-implantation	1780:1804	A mesothelium layer similar to the native mesothelium tissue could be obtained 21 days post-implantation, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
31547444	9	87	theme	best	1533:1536	arg1	cryogels					1540:1547	the best G cryogels	1529:1547	the best G cryogels for in vivo studies	1529:1567	Choosing the best G cryogels for in vivo studies, the cell/cryogel construct was used for the transplantation of allograft mesothelial cells for mesothelium reconstruction in rats.					
31547444	6	88	theme	cell	1070:1073	arg1	culture					1075:1081	the in vitro cell culture	1057:1081	the in vitro cell culture	1057:1081	From the in vitro cell culture, rat mesothelial cells showed quantitative cell viability in gelatin (G) and gelatin/HA (GH) cryogels.					
31547444	10	89	theme	native	1736:1741	arg1	tissue					1755:1760	the native mesothelium tissue	1732:1760	the native mesothelium tissue	1732:1760	A mesothelium layer similar to the native mesothelium tissue could be obtained 21 days post-implantation, based on hematoxylin and eosin (H&E) and immunohistochemical staining.					
31547444	6	90	theme	in	1061:1062	arg1	culture					1075:1081	the in vitro cell culture	1057:1081	the in vitro cell culture	1057:1081	From the in vitro cell culture, rat mesothelial cells showed quantitative cell viability in gelatin (G) and gelatin/HA (GH) cryogels.					
31547444	4	91	theme	kinetics	766:773	arg1	ratios					789:794	the pore size, porosity, water uptake kinetics, and swelling ratios	728:794	the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds	728:819	The incorporation of HA was found not to significantly change the pore size, porosity, water uptake kinetics, and swelling ratios of the cryogel scaffolds, but led to a faster scaffold degradation in the collagenase solution.					
31547444	1	92	theme	serosal	216:222	arg1	cavity					224:229	serosal cavity	216:229	serosal cavity	216:229	Mesothelial cells are specific epithelial cells that are lined in the serosal cavity and internal organs.					
31547444	5	93	theme	compressive	959:969	arg1	strain					979:984	strain	979:984	strain	979:984	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	5	93	theme	compressive	959:969	arg1	stress					971:976	the ultimate compressive stress	946:976	the ultimate compressive stress (strain)	946:985	Adding 5% HA in the composite cryogels also decreased the ultimate compressive stress (strain) and toughness of the scaffold, but enhanced the elastic modulus.					
31547444	3	94	theme	macroporous	442:452	arg1	scaffolds					454:462	macroporous scaffolds	442:462	macroporous scaffolds	442:462	Towards this end, we fabricated macroporous scaffolds from gelatin and gelatin/hyaluronic acid (HA) by cryogelation, and elucidated the influence of HA on cryogel properties and the cellular phenotype of mesothelial cells cultured within the 3D scaffolds.					
31547444	8	95	dep	proteins	1447:1454	arg1	E-cadherin					1456:1465	E-cadherin	1456:1465	E-cadherin	1456:1465	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	8	95	dep	proteins	1447:1454	arg1	proteins					1447:1454	key mesothelium proteins E-cadherin and calretinin	1431:1480	key mesothelium proteins E-cadherin and calretinin	1431:1480	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
31547444	8	95	dep	proteins	1447:1454	arg1	calretinin					1471:1480	calretinin	1471:1480	calretinin	1471:1480	The production of key mesothelium proteins E-cadherin and calretinin were also reduced in the GH cryogels.					
30349923	1	0	theme	whey	309:312	arg1	WPI					331:333	WPI	331:333	WPI	331:333	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	1	0	theme	whey	309:312	arg1	isolate					322:328	whey protein isolate	309:328	whey protein isolate (WPI)	309:334	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	7	1	theme	oleogel-structured	1156:1173	arg1	composite					1175:1183	this oleogel-structured composite	1151:1183	this oleogel-structured composite incorporating β-CD-fish oil complexes	1151:1221	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	0	2	theme	fish	72:75	arg1	oil					77:79	fish oil	72:79	fish oil	72:79	Oleogel-structured composite for the stabilization of ω3 fatty acids in fish oil.					
30349923	1	3	theme	protein	314:320	arg1	WPI					331:333	WPI	331:333	WPI	331:333	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	1	3	theme	protein	314:320	arg1	isolate					322:328	whey protein isolate	309:328	whey protein isolate (WPI)	309:334	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	1	4	theme	fish	148:151	arg1	oil					153:155	fish oil	148:155	fish oil	148:155	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	1	5	theme	isolate	322:328	arg1	layer					300:304	a layer	298:304	a layer of whey protein isolate (WPI)	298:334	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	4	6	theme	UVC	745:747	arg1	exposure					755:762	UVC light exposure	745:762	UVC light exposure	745:762	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	1	7	theme	oleogel	249:255	arg1	structure					257:265	an oleogel structure	246:265	an oleogel structure	246:265	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	7	8	theme	oil	1209:1211	arg1	complexes					1213:1221	β-CD-fish oil complexes	1199:1221	β-CD-fish oil complexes	1199:1221	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	5	9	theme	DHA	913:915	arg1	DHA					913:915	DHA	913:915	DHA	913:915	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	5	9	theme	DHA	913:915	arg1	%					895:895	more than 50 wt%	880:895	more than 50 wt% of both EPA and DHA	880:915	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	5	9	theme	DHA	913:915	arg1	EPA					905:907	EPA	905:907	EPA	905:907	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	4	10	theme	β-CD-fish	705:713	arg1	complex					719:725	β-CD-fish oil complex	705:725	β-CD-fish oil complex	705:725	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	5	11	theme	wt	893:894	arg1	DHA					913:915	DHA	913:915	DHA	913:915	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	5	11	theme	wt	893:894	arg1	%					895:895	more than 50 wt%	880:895	more than 50 wt% of both EPA and DHA	880:915	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	5	11	theme	wt	893:894	arg1	EPA					905:907	EPA	905:907	EPA	905:907	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	3	12	theme	emulsion	529:536	arg1	ζ-potential					483:493	ζ-potential	483:493	ζ-potential	483:493	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	3	12	theme	emulsion	529:536	arg1	size					474:477	Particle size	465:477	Particle size	465:477	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	6	13	from	samples	1087:1093	arg1	odor					1056:1059	odor	1056:1059	odor	1056:1059	In addition, sensory evaluation results showed a decreased fish oil odor in the WPI-coated oleogel samples.					
30349923	2	14	theme	β-CD-fish	354:362	arg1	complex					368:374	β-CD-fish oil complex	354:374	β-CD-fish oil complex	354:374	The formation of β-CD-fish oil complex was confirmed by thermogravimetric analysis and Fourier-transform infrared spectroscopy.					
30349923	2	15	theme	Fourier-transform	424:440	arg1	spectroscopy					451:462	Fourier-transform infrared spectroscopy	424:462	Fourier-transform infrared spectroscopy	424:462	The formation of β-CD-fish oil complex was confirmed by thermogravimetric analysis and Fourier-transform infrared spectroscopy.					
30349923	6	16	theme	fish	1047:1050	arg1	oil					1052:1054	a decreased fish oil	1035:1054	a decreased fish oil odor in the WPI-coated oleogel samples	1035:1093	In addition, sensory evaluation results showed a decreased fish oil odor in the WPI-coated oleogel samples.					
30349923	1	17	dep	encapsulation	106:118	arg1	the					102:104	the	102:104	the	102:104	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	6	18	theme	evaluation	1009:1018	arg1	results					1020:1026	sensory evaluation results	1001:1026	sensory evaluation results	1001:1026	In addition, sensory evaluation results showed a decreased fish oil odor in the WPI-coated oleogel samples.					
30349923	7	19	theme	decreased	1287:1295	arg1	odor					1318:1321	decreased undesirable fish oil odor	1287:1321	decreased undesirable fish oil odor	1287:1321	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	5	20	theme	exposure	946:953	arg1	period					955:960	a 4 h UV exposure period	937:960	a 4 h UV exposure period	937:960	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	0	21	from	stabilization	37:49	arg1	oil					77:79	fish oil	72:79	fish oil	72:79	Oleogel-structured composite for the stabilization of ω3 fatty acids in fish oil.					
30349923	1	22	from	stabilization	124:136	arg1	oil					153:155	fish oil	148:155	fish oil	148:155	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	6	23	theme	oleogel	1079:1085	arg1	samples					1087:1093	the WPI-coated oleogel samples	1064:1093	the WPI-coated oleogel samples	1064:1093	In addition, sensory evaluation results showed a decreased fish oil odor in the WPI-coated oleogel samples.					
30349923	7	24	theme	lipophilic	1260:1269	arg1	components					1271:1280	lipophilic components	1260:1280	lipophilic components with decreased undesirable fish oil odor	1260:1321	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	0	25	theme	Oleogel-structured	0:17	arg1	composite					19:27	Oleogel-structured composite	0:27	Oleogel-structured composite for the stabilization of ω3 fatty acids in fish oil.	0:80	Oleogel-structured composite for the stabilization of ω3 fatty acids in fish oil.					
30349923	4	26	theme	docosahexaenoic	820:834	arg1	DHA					842:844	DHA	842:844	DHA	842:844	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	4	26	theme	docosahexaenoic	820:834	arg1	acid					836:839	docosahexaenoic acid	820:839	docosahexaenoic acid (DHA)	820:845	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	3	27	theme	28-day	574:579	arg1	period					589:594	a 28-day storage period	572:594	a 28-day storage period	572:594	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	1	28	theme	multi-compartment	164:180	arg1	system					182:187	a multi-compartment system	162:187	a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI)	162:334	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	2	29	theme	thermogravimetric	393:409	arg1	analysis					411:418	thermogravimetric analysis	393:418	thermogravimetric analysis	393:418	The formation of β-CD-fish oil complex was confirmed by thermogravimetric analysis and Fourier-transform infrared spectroscopy.					
30349923	5	30	theme	4	939:939	arg1	h					941:941	h	941:941	h	941:941	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	1	31	from	encapsulation	106:118	arg1	oil					153:155	fish oil	148:155	fish oil	148:155	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	4	32	theme	oil	715:717	arg1	complex					719:725	β-CD-fish oil complex	705:725	β-CD-fish oil complex	705:725	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	5	33	theme	EPA	905:907	arg1	DHA					913:915	DHA	913:915	DHA	913:915	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	5	33	theme	EPA	905:907	arg1	%					895:895	more than 50 wt%	880:895	more than 50 wt% of both EPA and DHA	880:915	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	5	33	theme	EPA	905:907	arg1	EPA					905:907	EPA	905:907	EPA	905:907	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	2	34	theme	infrared	442:449	arg1	spectroscopy					451:462	Fourier-transform infrared spectroscopy	424:462	Fourier-transform infrared spectroscopy	424:462	The formation of β-CD-fish oil complex was confirmed by thermogravimetric analysis and Fourier-transform infrared spectroscopy.					
30349923	6	35	theme	WPI-coated	1068:1077	arg1	samples					1087:1093	the WPI-coated oleogel samples	1064:1093	the WPI-coated oleogel samples	1064:1093	In addition, sensory evaluation results showed a decreased fish oil odor in the WPI-coated oleogel samples.					
30349923	3	36	theme	NaCl	641:644	arg1	L-1					672:674	50-500 mg L-1	662:674	50-500 mg L-1	662:674	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	3	36	theme	NaCl	641:644	arg1	concentrations					646:659	NaCl concentrations	641:659	NaCl concentrations (50-500 mg L-1)	641:675	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	5	37	theme	UV	943:944	arg1	period					955:960	a 4 h UV exposure period	937:960	a 4 h UV exposure period	937:960	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	2	38	theme	complex	368:374	arg1	formation					341:349	The formation	337:349	The formation of β-CD-fish oil complex	337:374	The formation of β-CD-fish oil complex was confirmed by thermogravimetric analysis and Fourier-transform infrared spectroscopy.					
30349923	6	39	theme	sensory	1001:1007	arg1	results					1020:1026	sensory evaluation results	1001:1026	sensory evaluation results	1001:1026	In addition, sensory evaluation results showed a decreased fish oil odor in the WPI-coated oleogel samples.					
30349923	3	40	theme	different	602:610	arg1	conditions					615:624	different pH conditions	602:624	different pH conditions (pH 3.5-7)	602:635	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	3	40	theme	different	602:610	arg1	pH					627:628	pH 3.5-7	627:634	pH 3.5-7	627:634	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	1	41	theme	β-cyclodextrins	203:217	arg1	complexation					226:237	β-cyclodextrins (β-CD) complexation	203:237	β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI)	203:334	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	0	42	theme	fatty	57:61	arg1	acids					63:67	ω3 fatty acids	54:67	ω3 fatty acids	54:67	Oleogel-structured composite for the stabilization of ω3 fatty acids in fish oil.					
30349923	7	43	theme	undesirable	1297:1307	arg1	odor					1318:1321	decreased undesirable fish oil odor	1287:1321	decreased undesirable fish oil odor	1287:1321	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	3	44	theme	storage	581:587	arg1	period					589:594	a 28-day storage period	572:594	a 28-day storage period	572:594	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	6	45	theme	odor	1056:1059	arg1	oil					1052:1054	a decreased fish oil	1035:1054	a decreased fish oil odor in the WPI-coated oleogel samples	1035:1093	In addition, sensory evaluation results showed a decreased fish oil odor in the WPI-coated oleogel samples.					
30349923	0	46	theme	ω3	54:55	arg1	acids					63:67	ω3 fatty acids	54:67	ω3 fatty acids	54:67	Oleogel-structured composite for the stabilization of ω3 fatty acids in fish oil.					
30349923	7	47	with	components	1271:1280	arg1	odor					1318:1321	decreased undesirable fish oil odor	1287:1321	decreased undesirable fish oil odor	1287:1321	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	7	48	from	study	1127:1131	arg1	results					1109:1115	the results	1105:1115	the results from this study	1105:1131	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	5	49	theme	WPI-coated	969:978	arg1	system					980:985	the WPI-coated system	965:985	the WPI-coated system	965:985	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	6	50	from	odor	1056:1059	arg1	samples					1087:1093	the WPI-coated oleogel samples	1064:1093	the WPI-coated oleogel samples	1064:1093	In addition, sensory evaluation results showed a decreased fish oil odor in the WPI-coated oleogel samples.					
30349923	4	51	theme	WPI-coated	682:691	arg1	oleogel					693:699	The WPI-coated oleogel	678:699	The WPI-coated oleogel	678:699	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	7	52	theme	β-CD-fish	1199:1207	arg1	complexes					1213:1221	β-CD-fish oil complexes	1199:1221	β-CD-fish oil complexes	1199:1221	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	3	53	theme	mg	669:670	arg1	L-1					672:674	50-500 mg L-1	662:674	50-500 mg L-1	662:674	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	3	53	theme	mg	669:670	arg1	concentrations					646:659	NaCl concentrations	641:659	NaCl concentrations (50-500 mg L-1)	641:675	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	7	54	theme	fish	1309:1312	arg1	odor					1318:1321	decreased undesirable fish oil odor	1287:1321	decreased undesirable fish oil odor	1287:1321	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	4	55	theme	eicosapentaenoic	788:803	arg1	acid					805:808	their eicosapentaenoic acid	782:808	their eicosapentaenoic acid (EPA)	782:814	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	4	55	theme	eicosapentaenoic	788:803	arg1	EPA					811:813	EPA	811:813	EPA	811:813	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	3	56	theme	Particle	465:472	arg1	size					474:477	Particle size	465:477	Particle size	465:477	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	6	57	theme	decreased	1037:1045	arg1	oil					1052:1054	a decreased fish oil	1035:1054	a decreased fish oil odor in the WPI-coated oleogel samples	1035:1093	In addition, sensory evaluation results showed a decreased fish oil odor in the WPI-coated oleogel samples.					
30349923	4	58	theme	light	749:753	arg1	exposure					755:762	UVC light exposure	745:762	UVC light exposure	745:762	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	7	59	theme	oil	1314:1316	arg1	odor					1318:1321	decreased undesirable fish oil odor	1287:1321	decreased undesirable fish oil odor	1287:1321	Overall, the results from this study demonstrated that this oleogel-structured composite incorporating β-CD-fish oil complexes can be formed successfully to retain lipophilic components with decreased undesirable fish oil odor.					
30349923	3	60	theme	oil-in-water	516:527	arg1	emulsion					529:536	the oleogel-based oil-in-water emulsion	498:536	the oleogel-based oil-in-water emulsion	498:536	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	1	61	theme	β-CD	220:223	arg1	complexation					226:237	β-cyclodextrins (β-CD) complexation	203:237	β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI)	203:334	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	2	62	theme	oil	364:366	arg1	complex					368:374	β-CD-fish oil complex	354:374	β-CD-fish oil complex	354:374	The formation of β-CD-fish oil complex was confirmed by thermogravimetric analysis and Fourier-transform infrared spectroscopy.					
30349923	3	63	theme	pH	612:613	arg1	conditions					615:624	different pH conditions	602:624	different pH conditions (pH 3.5-7)	602:635	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	3	63	theme	pH	612:613	arg1	pH					627:628	pH 3.5-7	627:634	pH 3.5-7	627:634	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	3	64	theme	oleogel-based	502:514	arg1	emulsion					529:536	the oleogel-based oil-in-water emulsion	498:536	the oleogel-based oil-in-water emulsion	498:536	Particle size and ζ-potential of the oleogel-based oil-in-water emulsion did not change significantly over a 28-day storage period under different pH conditions (pH 3.5-7) and NaCl concentrations (50-500 mg L-1).					
30349923	4	65	dep	acid	805:808	arg1	contents					847:854	contents	847:854	contents	847:854	The WPI-coated oleogel and β-CD-fish oil complex were subjected to UVC light exposure and quantified by their eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents.					
30349923	0	66	theme	acids	63:67	arg1	stabilization					37:49	the stabilization	33:49	the stabilization of ω3 fatty acids in fish oil	33:79	Oleogel-structured composite for the stabilization of ω3 fatty acids in fish oil.					
30349923	5	67	theme	h	941:941	arg1	period					955:960	a 4 h UV exposure period	937:960	a 4 h UV exposure period	937:960	Results indicated that more than 50 wt% of both EPA and DHA were retained after a 4 h UV exposure period in the WPI-coated system.					
30349923	1	68	theme	É·3	141:143	arg1	stabilization					124:136	stabilization	124:136	stabilization	124:136	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30349923	1	68	theme	É·3	141:143	arg1	encapsulation					106:118	encapsulation	106:118	encapsulation	106:118	This study examined the encapsulation and stabilization of É·3 in fish oil into a multi-compartment system consisting of β-cyclodextrins (β-CD) complexation within an oleogel structure, which was further coated with a layer of whey protein isolate (WPI).					
30778123	8	0	theme	gonadal	1165:1171	arg1	depots					1192:1197	both gonadal and mesenteric fat depots	1160:1197	both gonadal and mesenteric fat depots of Xylt2-/- mice	1160:1214	Histologically, the adipocytes are reduced in size and number in both gonadal and mesenteric fat depots of Xylt2-/- mice.					
30778123	4	1	theme	GAGs	588:591	arg1	role					567:570	the role	563:570	the role of ECM sulfated GAGs in adipose tissue homeostasis	563:621	The purpose of this study was to determine the role of ECM sulfated GAGs in adipose tissue homeostasis.					
30778123	7	2	contain	have	1019:1022	arg2	weight					1033:1038	low body weight	1024:1038	low body weight due to overall reductions in abdominal fat deposition	1024:1092	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	7	2	contain	have	1019:1022	arg1	mice					1014:1017	RESULTS Xylt2-/- mice	997:1017	RESULTS Xylt2-/- mice	997:1017	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	11	3	theme	adipose	1653:1659	arg1	tissue					1661:1666	gonadal adipose tissue	1645:1666	gonadal adipose tissue	1645:1666	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	10	4	theme	inflammatory	1416:1427	arg1	cells					1429:1433	inflammatory cells	1416:1433	inflammatory cells	1416:1433	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	2	5	theme	resident	373:380	arg1	cells					382:386	resident cells	373:386	resident cells	373:386	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	8	6	theme	mesenteric	1177:1186	arg1	depots					1192:1197	both gonadal and mesenteric fat depots	1160:1197	both gonadal and mesenteric fat depots of Xylt2-/- mice	1160:1214	Histologically, the adipocytes are reduced in size and number in both gonadal and mesenteric fat depots of Xylt2-/- mice.					
30778123	6	7	theme	flow	934:937	arg1	cytometry					939:947	flow cytometry	934:947	flow cytometry	934:947	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	4	8	theme	adipose	596:602	arg1	homeostasis					611:621	adipose tissue homeostasis	596:621	adipose tissue homeostasis	596:621	The purpose of this study was to determine the role of ECM sulfated GAGs in adipose tissue homeostasis.					
30778123	11	9	theme	impaired	1692:1699	arg1	differentiation					1712:1726	impaired adipogenic differentiation	1692:1726	impaired adipogenic differentiation	1692:1726	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	5	10	theme	deficient	681:689	arg1	mice					676:679	mice	676:679	mice deficient in xylosyltransferase 2 (Xylt2-/-)	676:724	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	2	11	theme	spatial	301:307	arg1	signals					322:328	spatial and temporal signals	301:328	spatial and temporal signals for ECM organization and interactions with resident cells	301:386	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	12	12	theme	GAG	1792:1794	arg1	enzyme					1805:1810	the key GAG assembly enzyme	1784:1810	the key GAG assembly enzyme	1784:1810	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	8	13	from	number	1150:1155	arg1	depots					1192:1197	both gonadal and mesenteric fat depots	1160:1197	both gonadal and mesenteric fat depots of Xylt2-/- mice	1160:1214	Histologically, the adipocytes are reduced in size and number in both gonadal and mesenteric fat depots of Xylt2-/- mice.					
30778123	8	14	theme	Xylt2-/-	1202:1209	arg1	mice					1211:1214	Xylt2-/- mice	1202:1214	Xylt2-/- mice	1202:1214	Histologically, the adipocytes are reduced in size and number in both gonadal and mesenteric fat depots of Xylt2-/- mice.					
30778123	10	15	theme	vascular	1541:1548	arg1	cells					1562:1566	adipose tissue vascular endothelial cells	1526:1566	adipose tissue vascular endothelial cells	1526:1566	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	5	16	theme	metabolic	651:659	arg1	homeostasis					661:671	metabolic homeostasis	651:671	metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-)	651:724	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	2	17	theme	temporal	313:320	arg1	signals					322:328	spatial and temporal signals	301:328	spatial and temporal signals for ECM organization and interactions with resident cells	301:386	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	6	18	theme	stem	985:988	arg1	cells					990:994	mesenchymal stem cells	973:994	mesenchymal stem cells	973:994	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	14	19	theme	development	2196:2206	arg1	control					2120:2126	control	2120:2126	control of adipose precursor development, tissue inflammation, and vascular development	2120:2206	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	9	20	theme	glucose	1245:1251	arg1	mice					1236:1239	these mice	1230:1239	these mice	1230:1239	In addition, these mice are glucose intolerant, insulin resistant, and have increased serum triglycerides as compared to Xylt2 + / + control mice.					
30778123	9	20	theme	glucose	1245:1251	arg1	intolerant					1253:1262	glucose intolerant	1245:1262	glucose intolerant	1245:1262	In addition, these mice are glucose intolerant, insulin resistant, and have increased serum triglycerides as compared to Xylt2 + / + control mice.					
30778123	7	21	theme	due	1040:1042	arg1	weight					1033:1038	low body weight	1024:1038	low body weight due to overall reductions in abdominal fat deposition	1024:1092	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	7	22	theme	abdominal	1069:1077	arg1	deposition					1083:1092	abdominal fat deposition	1069:1092	abdominal fat deposition	1069:1092	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	7	23	theme	body	1028:1031	arg1	weight					1033:1038	low body weight	1024:1038	low body weight due to overall reductions in abdominal fat deposition	1024:1092	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	12	24	theme	Decreased	1750:1758	arg1	XylT2					1812:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2	1738:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2	1738:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	13	25	theme	pool	1950:1953	arg1	Accumulation					1910:1921	Accumulation	1910:1921	Accumulation of an adipocytic precursor pool of cells	1910:1962	Accumulation of an adipocytic precursor pool of cells is discovered indicating an interruption in differentiation.					
30778123	12	26	theme	due	1765:1767	arg1	XylT2					1812:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2	1738:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2	1738:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	13	27	theme	cells	1958:1962	arg1	pool					1950:1953	an adipocytic precursor pool	1926:1953	an adipocytic precursor pool of cells	1926:1962	Accumulation of an adipocytic precursor pool of cells is discovered indicating an interruption in differentiation.					
30778123	6	28	dep	in	953:954	arg1	vitro					956:960	vitro	956:960	vitro	956:960	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	2	29	theme	sulfated	258:265	arg1	glycosaminoglycans					267:284	their sulfated glycosaminoglycans	252:284	their sulfated glycosaminoglycans (GAGs)	252:291	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	2	29	theme	sulfated	258:265	arg1	GAGs					287:290	GAGs	287:290	GAGs	287:290	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	7	30	theme	Xylt2-/-	1005:1012	arg1	mice					1014:1017	RESULTS Xylt2-/- mice	997:1017	RESULTS Xylt2-/- mice	997:1017	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	9	31	theme	increased	1293:1301	arg1	triglycerides					1309:1321	increased serum triglycerides	1293:1321	increased serum triglycerides	1293:1321	In addition, these mice are glucose intolerant, insulin resistant, and have increased serum triglycerides as compared to Xylt2 + / + control mice.					
30778123	1	32	theme	adipose	172:178	arg1	homeostasis					187:197	adipose tissue homeostasis	172:197	adipose tissue homeostasis	172:197	BACKGROUND/OBJECTIVES The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood.					
30778123	8	33	from	size	1141:1144	arg1	depots					1192:1197	both gonadal and mesenteric fat depots	1160:1197	both gonadal and mesenteric fat depots of Xylt2-/- mice	1160:1214	Histologically, the adipocytes are reduced in size and number in both gonadal and mesenteric fat depots of Xylt2-/- mice.					
30778123	10	34	theme	factors	1469:1475	arg1	enrichment					1439:1448	enrichment	1439:1448	enrichment of proinflammatory factors IL6 and IL1β	1439:1488	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	10	34	theme	factors	1469:1475	arg1	cells					1429:1433	inflammatory cells	1416:1433	inflammatory cells	1416:1433	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	13	35	theme	adipocytic	1929:1938	arg1	pool					1950:1953	an adipocytic precursor pool	1926:1953	an adipocytic precursor pool of cells	1926:1962	Accumulation of an adipocytic precursor pool of cells is discovered indicating an interruption in differentiation.					
30778123	5	36	theme	histologic	743:752	arg1	analyses					754:761	histologic analyses	743:761	histologic analyses	743:761	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	14	37	theme	development	2149:2159	arg1	control					2120:2126	control	2120:2126	control of adipose precursor development, tissue inflammation, and vascular development	2120:2206	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	2	38	theme	cytokine	419:426	arg1	activity					428:435	cytokine activity	419:435	cytokine activity	419:435	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	10	39	dep	factors	1469:1475	arg1	IL6					1477:1479	IL6	1477:1479	IL6	1477:1479	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	10	39	dep	factors	1469:1475	arg1	factors					1469:1475	proinflammatory factors IL6 and IL1β	1453:1488	proinflammatory factors IL6 and IL1β	1453:1488	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	10	39	dep	factors	1469:1475	arg1	IL1β					1485:1488	IL1β	1485:1488	IL1β	1485:1488	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	11	40	from	marrow	1677:1682	arg1	cells					1634:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells	1577:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow	1577:1682	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	0	41	from	lipodystrophy	24:36	arg1	mice					73:76	xylosyltransferase II deficient mice	41:76	xylosyltransferase II deficient mice	41:76	Adipose tissue loss and lipodystrophy in xylosyltransferase II deficient mice.					
30778123	14	42	theme	adipose	2131:2137	arg1	development					2149:2159	adipose precursor development	2131:2159	adipose precursor development	2131:2159	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	0	43	theme	Adipose	0:6	arg1	tissue					8:13	Adipose tissue loss and lipodystrophy	0:36	tissue	8:13	Adipose tissue loss and lipodystrophy in xylosyltransferase II deficient mice.					
30778123	9	44	theme	Xylt2 + / + control	1338:1356	arg1	mice					1358:1361	Xylt2 + / + control mice	1338:1361	Xylt2 + / + control mice	1338:1361	In addition, these mice are glucose intolerant, insulin resistant, and have increased serum triglycerides as compared to Xylt2 + / + control mice.					
30778123	14	45	theme	adipose	2036:2042	arg1	tissue					2044:2049	adipose tissue	2036:2049	adipose tissue GAGs	2036:2054	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	5	46	theme	glucose	835:841	arg1	test					853:856	glucose tolerance test	835:856	glucose tolerance test	835:856	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	12	47	theme	unique	1881:1886	arg1	model					1903:1907	a unique lipodystrophic model	1879:1907	a unique lipodystrophic model	1879:1907	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	6	48	theme	adipocyte	891:899	arg1	precursors					901:910	adipocyte precursors	891:910	adipocyte precursors	891:910	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	1	49	theme	extracellular	118:130	arg1	ECM					140:142	ECM	140:142	ECM	140:142	BACKGROUND/OBJECTIVES The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood.					
30778123	1	49	theme	extracellular	118:130	arg1	matrix					132:137	extracellular matrix	118:137	extracellular matrix (ECM)	118:143	BACKGROUND/OBJECTIVES The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood.					
30778123	0	50	theme	deficient	63:71	arg1	mice					73:76	xylosyltransferase II deficient mice	41:76	xylosyltransferase II deficient mice	41:76	Adipose tissue loss and lipodystrophy in xylosyltransferase II deficient mice.					
30778123	5	51	theme	body	796:799	arg1	measurements					817:828	whole body fat composition measurements	790:828	whole body fat composition measurements	790:828	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	12	52	theme	state	1840:1844	arg1	stores					1861:1866	reduced steady state adipose tissue stores	1825:1866	reduced steady state adipose tissue stores	1825:1866	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	4	53	theme	study	540:544	arg1	purpose					524:530	The purpose	520:530	The purpose of this study	520:544	The purpose of this study was to determine the role of ECM sulfated GAGs in adipose tissue homeostasis.					
30778123	10	54	theme	tissue	1534:1539	arg1	cells					1562:1566	adipose tissue vascular endothelial cells	1526:1566	adipose tissue vascular endothelial cells	1526:1566	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	5	55	theme	expression	769:778	arg1	analyses					780:787	gene expression analyses	764:787	gene expression analyses	764:787	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	11	56	theme	mesenchymal	1617:1627	arg1	cells					1634:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells	1577:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow	1577:1682	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	5	57	theme	composition	805:815	arg1	measurements					817:828	whole body fat composition measurements	790:828	whole body fat composition measurements	790:828	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	5	58	from	homeostasis	661:671	arg1	mice					676:679	mice	676:679	mice deficient in xylosyltransferase 2 (Xylt2-/-)	676:724	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	6	59	theme	Adipose	859:865	arg1	inflammation					874:885	Adipose tissue inflammation	859:885	Adipose tissue inflammation	859:885	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	12	60	theme	tissue	1854:1859	arg1	stores					1861:1866	reduced steady state adipose tissue stores	1825:1866	reduced steady state adipose tissue stores	1825:1866	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	3	61	theme	adipose	492:498	arg1	homeostasis					507:517	adipose tissue homeostasis	492:517	adipose tissue homeostasis	492:517	Therefore, PGs and their GAGs could be significant to adipose tissue homeostasis.					
30778123	7	62	from	reductions	1055:1064	arg1	deposition					1083:1092	abdominal fat deposition	1069:1092	abdominal fat deposition	1069:1092	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	10	63	theme	adipose	1381:1387	arg1	niche					1396:1400	the adipose tissue niche	1377:1400	the adipose tissue niche	1377:1400	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	6	64	theme	in	953:954	arg1	culture					962:968	in vitro culture	953:968	in vitro culture of mesenchymal stem cells	953:994	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	12	65	theme	reduced	1825:1831	arg1	stores					1861:1866	reduced steady state adipose tissue stores	1825:1866	reduced steady state adipose tissue stores	1825:1866	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	10	66	theme	endothelial	1550:1560	arg1	cells					1562:1566	adipose tissue vascular endothelial cells	1526:1566	adipose tissue vascular endothelial cells	1526:1566	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	11	67	theme	bone	1672:1675	arg1	marrow					1677:1682	bone marrow	1672:1682	bone marrow	1672:1682	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	14	68	theme	adipose	2092:2098	arg1	tissue					2100:2105	adipose tissue	2092:2105	adipose tissue	2092:2105	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	12	69	theme	key	1788:1790	arg1	enzyme					1805:1810	the key GAG assembly enzyme	1784:1810	the key GAG assembly enzyme	1784:1810	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	2	70	with	interactions	355:366	arg1	cells					382:386	resident cells	373:386	resident cells	373:386	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	4	71	theme	sulfated	579:586	arg1	GAGs					588:591	ECM sulfated GAGs	575:591	ECM sulfated GAGs	575:591	The purpose of this study was to determine the role of ECM sulfated GAGs in adipose tissue homeostasis.					
30778123	9	72	from	intolerant	1253:1262	arg1	addition					1220:1227	addition	1220:1227	addition	1220:1227	In addition, these mice are glucose intolerant, insulin resistant, and have increased serum triglycerides as compared to Xylt2 + / + control mice.					
30778123	12	73	theme	assembly	1796:1803	arg1	enzyme					1805:1810	the key GAG assembly enzyme	1784:1810	the key GAG assembly enzyme	1784:1810	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	11	74	theme	adipogenic	1701:1710	arg1	differentiation					1712:1726	impaired adipogenic differentiation	1692:1726	impaired adipogenic differentiation	1692:1726	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	5	75	theme	Adipose	632:638	arg1	tissue					640:645	METHODS Adipose tissue	624:645	METHODS Adipose tissue	624:645	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	1	76	theme	tissue	180:185	arg1	homeostasis					187:197	adipose tissue homeostasis	172:197	adipose tissue homeostasis	172:197	BACKGROUND/OBJECTIVES The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood.					
30778123	8	77	theme	fat	1188:1190	arg1	depots					1192:1197	both gonadal and mesenteric fat depots	1160:1197	both gonadal and mesenteric fat depots of Xylt2-/- mice	1160:1214	Histologically, the adipocytes are reduced in size and number in both gonadal and mesenteric fat depots of Xylt2-/- mice.					
30778123	2	78	theme	ECM	334:336	arg1	organization					338:349	ECM organization	334:349	ECM organization	334:349	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	14	79	from	homeostasis	2077:2087	arg1	important					2060:2068	important	2060:2068	important	2060:2068	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	0	80	theme	xylosyltransferase	41:58	arg1	mice					73:76	xylosyltransferase II deficient mice	41:76	xylosyltransferase II deficient mice	41:76	Adipose tissue loss and lipodystrophy in xylosyltransferase II deficient mice.					
30778123	5	81	theme	tolerance	843:851	arg1	test					853:856	glucose tolerance test	835:856	glucose tolerance test	835:856	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	8	82	theme	mice	1211:1214	arg1	depots					1192:1197	both gonadal and mesenteric fat depots	1160:1197	both gonadal and mesenteric fat depots of Xylt2-/- mice	1160:1214	Histologically, the adipocytes are reduced in size and number in both gonadal and mesenteric fat depots of Xylt2-/- mice.					
30778123	6	83	theme	cells	990:994	arg1	cytometry					939:947	flow cytometry	934:947	flow cytometry	934:947	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	6	83	theme	cells	990:994	arg1	culture					962:968	in vitro culture	953:968	in vitro culture of mesenchymal stem cells	953:994	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	7	84	theme	fat	1079:1081	arg1	deposition					1083:1092	abdominal fat deposition	1069:1092	abdominal fat deposition	1069:1092	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	6	85	theme	mesenchymal	973:983	arg1	cells					990:994	mesenchymal stem cells	973:994	mesenchymal stem cells	973:994	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	14	86	theme	vascular	2187:2194	arg1	development					2196:2206	vascular development	2187:2206	vascular development	2187:2206	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	1	87	dep	BACKGROUND/OBJECTIVES	79:99	arg1	ECM					140:142	ECM	140:142	ECM	140:142	BACKGROUND/OBJECTIVES The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood.					
30778123	1	87	dep	BACKGROUND/OBJECTIVES	79:99	arg1	cellular					105:112	cellular	105:112	cellular	105:112	BACKGROUND/OBJECTIVES The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood.					
30778123	1	87	dep	BACKGROUND/OBJECTIVES	79:99	arg1	matrix					132:137	extracellular matrix	118:137	extracellular matrix (ECM)	118:143	BACKGROUND/OBJECTIVES The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood.					
30778123	13	88	theme	precursor	1940:1948	arg1	pool					1950:1953	an adipocytic precursor pool	1926:1953	an adipocytic precursor pool of cells	1926:1962	Accumulation of an adipocytic precursor pool of cells is discovered indicating an interruption in differentiation.					
30778123	7	89	theme	overall	1047:1053	arg1	reductions					1055:1064	overall reductions	1047:1064	overall reductions in abdominal fat deposition	1047:1092	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	10	90	contain	have	1511:1514	arg1	mice					1501:1504	these mice	1495:1504	these mice	1495:1504	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	10	90	contain	have	1511:1514	arg2	loss					1518:1521	a loss	1516:1521	a loss of adipose tissue vascular endothelial cells	1516:1566	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	12	91	theme	CONCLUSIONS	1738:1748	arg1	XylT2					1812:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2	1738:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2	1738:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	5	92	theme	gene	764:767	arg1	analyses					780:787	gene expression analyses	764:787	gene expression analyses	764:787	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	7	93	theme	low	1024:1026	arg1	weight					1033:1038	low body weight	1024:1038	low body weight due to overall reductions in abdominal fat deposition	1024:1092	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	12	94	theme	GAGs	1760:1763	arg1	XylT2					1812:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2	1738:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2	1738:1816	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	13	95	from	interruption	1992:2003	arg1	differentiation					2008:2022	differentiation	2008:2022	differentiation	2008:2022	Accumulation of an adipocytic precursor pool of cells is discovered indicating an interruption in differentiation.					
30778123	0	96	from	tissue	8:13	arg1	mice					73:76	xylosyltransferase II deficient mice	41:76	xylosyltransferase II deficient mice	41:76	Adipose tissue loss and lipodystrophy in xylosyltransferase II deficient mice.					
30778123	14	97	theme	inflammation	2169:2180	arg1	control					2120:2126	control	2120:2126	control of adipose precursor development, tissue inflammation, and vascular development	2120:2206	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	1	98	dep	cellular	105:112	arg1	interactions					145:156	interactions	145:156	interactions	145:156	BACKGROUND/OBJECTIVES The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood.					
30778123	1	98	dep	cellular	105:112	arg1	The					101:103	The	101:103	The	101:103	BACKGROUND/OBJECTIVES The cellular and extracellular matrix (ECM) interactions that regulate adipose tissue homeostasis are incompletely understood.					
30778123	9	99	theme	serum	1303:1307	arg1	triglycerides					1309:1321	increased serum triglycerides	1293:1321	increased serum triglycerides	1293:1321	In addition, these mice are glucose intolerant, insulin resistant, and have increased serum triglycerides as compared to Xylt2 + / + control mice.					
30778123	7	100	theme	RESULTS	997:1003	arg1	mice					1014:1017	RESULTS Xylt2-/- mice	997:1017	RESULTS Xylt2-/- mice	997:1017	RESULTS Xylt2-/- mice have low body weight due to overall reductions in abdominal fat deposition.					
30778123	10	101	theme	proinflammatory	1453:1467	arg1	IL6					1477:1479	IL6	1477:1479	IL6	1477:1479	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	10	101	theme	proinflammatory	1453:1467	arg1	factors					1469:1475	proinflammatory factors IL6 and IL1β	1453:1488	proinflammatory factors IL6 and IL1β	1453:1488	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	10	101	theme	proinflammatory	1453:1467	arg1	IL1β					1485:1488	IL1β	1485:1488	IL1β	1485:1488	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	5	102	theme	METHODS	624:630	arg1	tissue					640:645	METHODS Adipose tissue	624:645	METHODS Adipose tissue	624:645	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	14	103	theme	precursor	2139:2147	arg1	development					2149:2159	adipose precursor development	2131:2159	adipose precursor development	2131:2159	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	4	104	theme	tissue	604:609	arg1	homeostasis					611:621	adipose tissue homeostasis	596:621	adipose tissue homeostasis	596:621	The purpose of this study was to determine the role of ECM sulfated GAGs in adipose tissue homeostasis.					
30778123	9	105	contain	have	1288:1291	arg1	mice					1236:1239	these mice	1230:1239	these mice	1230:1239	In addition, these mice are glucose intolerant, insulin resistant, and have increased serum triglycerides as compared to Xylt2 + / + control mice.					
30778123	9	105	contain	have	1288:1291	arg1	intolerant					1253:1262	glucose intolerant	1245:1262	glucose intolerant	1245:1262	In addition, these mice are glucose intolerant, insulin resistant, and have increased serum triglycerides as compared to Xylt2 + / + control mice.					
30778123	9	105	contain	have	1288:1291	arg2	triglycerides					1309:1321	increased serum triglycerides	1293:1321	increased serum triglycerides	1293:1321	In addition, these mice are glucose intolerant, insulin resistant, and have increased serum triglycerides as compared to Xylt2 + / + control mice.					
30778123	14	106	theme	tissue	2044:2049	arg1	GAGs					2051:2054	adipose tissue GAGs	2036:2054	adipose tissue GAGs	2036:2054	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	2	107	theme	growth	401:406	arg1	factor					408:413	growth factor	401:413	growth factor	401:413	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	4	108	from	role	567:570	arg1	homeostasis					611:621	adipose tissue homeostasis	596:621	adipose tissue homeostasis	596:621	The purpose of this study was to determine the role of ECM sulfated GAGs in adipose tissue homeostasis.					
30778123	12	109	theme	lipodystrophic	1888:1901	arg1	model					1903:1907	a unique lipodystrophic model	1879:1907	a unique lipodystrophic model	1879:1907	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	14	110	from	important	2060:2068	arg1	homeostasis					2077:2087	the homeostasis	2073:2087	the homeostasis of adipose tissue	2073:2105	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	11	111	theme	gonadal	1645:1651	arg1	tissue					1661:1666	gonadal adipose tissue	1645:1666	gonadal adipose tissue	1645:1666	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	4	112	theme	ECM	575:577	arg1	GAGs					588:591	ECM sulfated GAGs	575:591	ECM sulfated GAGs	575:591	The purpose of this study was to determine the role of ECM sulfated GAGs in adipose tissue homeostasis.					
30778123	5	113	theme	whole	790:794	arg1	measurements					817:828	whole body fat composition measurements	790:828	whole body fat composition measurements	790:828	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	5	114	from	tissue	640:645	arg1	mice					676:679	mice	676:679	mice deficient in xylosyltransferase 2 (Xylt2-/-)	676:724	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	2	115	with	organization	338:349	arg1	cells					382:386	resident cells	373:386	resident cells	373:386	Proteoglycans (PGs) and their sulfated glycosaminoglycans (GAGs) provide spatial and temporal signals for ECM organization and interactions with resident cells by impacting growth factor and cytokine activity.					
30778123	14	116	theme	tissue	2162:2167	arg1	inflammation					2169:2180	tissue inflammation	2162:2180	tissue inflammation	2162:2180	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	11	117	from	tissue	1661:1666	arg1	cells					1634:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells	1577:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow	1577:1682	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	14	118	theme	tissue	2100:2105	arg1	homeostasis					2077:2087	the homeostasis	2073:2087	the homeostasis of adipose tissue	2073:2105	Therefore, adipose tissue GAGs are important in the homeostasis of adipose tissue by mediating control of adipose precursor development, tissue inflammation, and vascular development.					
30778123	11	119	theme	deficient	1607:1615	arg1	cells					1634:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells	1577:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow	1577:1682	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	5	120	theme	fat	801:803	arg1	measurements					817:828	whole body fat composition measurements	790:828	whole body fat composition measurements	790:828	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	5	121	from	deficient	681:689	arg1	xylosyltransferase					694:711	xylosyltransferase 2	694:713	xylosyltransferase 2 (Xylt2-/-)	694:724	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	5	121	from	deficient	681:689	arg1	Xylt2-/-					716:723	Xylt2-/-	716:723	Xylt2-/-	716:723	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30778123	6	122	theme	tissue	867:872	arg1	inflammation					874:885	Adipose tissue inflammation	859:885	Adipose tissue inflammation	859:885	Adipose tissue inflammation and adipocyte precursors were characterized by flow cytometry and in vitro culture of mesenchymal stem cells.					
30778123	12	123	theme	steady	1833:1838	arg1	stores					1861:1866	reduced steady state adipose tissue stores	1825:1866	reduced steady state adipose tissue stores	1825:1866	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	10	124	theme	adipose	1526:1532	arg1	cells					1562:1566	adipose tissue vascular endothelial cells	1526:1566	adipose tissue vascular endothelial cells	1526:1566	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	12	125	theme	adipose	1846:1852	arg1	stores					1861:1866	reduced steady state adipose tissue stores	1825:1866	reduced steady state adipose tissue stores	1825:1866	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	10	126	theme	tissue	1389:1394	arg1	niche					1396:1400	the adipose tissue niche	1377:1400	the adipose tissue niche	1377:1400	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	3	127	theme	tissue	500:505	arg1	homeostasis					507:517	adipose tissue homeostasis	492:517	adipose tissue homeostasis	492:517	Therefore, PGs and their GAGs could be significant to adipose tissue homeostasis.					
30778123	0	128	dep	tissue	8:13	arg1	loss					15:18	loss	15:18	loss	15:18	Adipose tissue loss and lipodystrophy in xylosyltransferase II deficient mice.					
30778123	12	129	theme	enzyme	1805:1810	arg1	loss					1776:1779	the loss	1772:1779	the loss of the key GAG assembly enzyme	1772:1810	CONCLUSIONS Decreased GAGs due to the loss of the key GAG assembly enzyme XylT2 causes reduced steady state adipose tissue stores leading to a unique lipodystrophic model.					
30778123	10	130	theme	cells	1562:1566	arg1	loss					1518:1521	a loss	1516:1521	a loss of adipose tissue vascular endothelial cells	1516:1566	Furthermore, the adipose tissue niche has increased inflammatory cells and enrichment of proinflammatory factors IL6 and IL1β, and these mice also have a loss of adipose tissue vascular endothelial cells.					
30778123	11	131	theme	stem	1629:1632	arg1	cells					1634:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells	1577:1638	xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow	1577:1682	Lastly, xylosyltransferease-2 (XylT2) deficient mesenchymal stem cells from gonadal adipose tissue and bone marrow exhibit impaired adipogenic differentiation in vitro.					
30778123	5	132	from	xylosyltransferase	694:711	arg1	deficient					681:689	deficient	681:689	deficient	681:689	METHODS Adipose tissue and metabolic homeostasis in mice deficient in xylosyltransferase 2 (Xylt2-/-) were examined by histologic analyses, gene expression analyses, whole body fat composition measurements, and glucose tolerance test.					
30648942	11	0	theme	genomic	1820:1826	arg1	characteristics					1828:1842	genomic characteristics	1820:1842	genomic characteristics	1820:1842	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	9	1	theme	summed	1385:1390	arg1	 0					1381:1382	 0	1381:1382	 0	1381:1382	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	1	theme	summed	1385:1390	arg1	feature					1392:1398	summed feature 3	1385:1400	summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c)	1385:1431	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	2	theme	isoprenoid	1481:1490	arg1	quinone					1492:1498	the dominant isoprenoid quinone	1468:1498	the dominant isoprenoid quinone	1468:1498	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	2	theme	isoprenoid	1481:1490	arg1	MK-7					1459:1462	MK-7	1459:1462	MK-7	1459:1462	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	2	theme	isoprenoid	1481:1490	arg1	 0					1381:1382	 0	1381:1382	 0	1381:1382	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	2	3	theme	novel	266:270	arg1	strain					282:287	a novel bacterial strain	264:287	a novel bacterial strain named M46T	264:298	Assessment of the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching, revealed the presence of a novel bacterial strain named M46T.					
30648942	4	4	theme	S.	862:863	arg1	CR11T					875:879	S. canadense CR11T	862:879	S. canadense CR11T	862:879	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	4	5	theme	Sphingobacterium	674:689	arg1	analysis					728:735	Sphingobacterium detergens6.2 ST. Multilocus sequence analysis	674:735	Sphingobacterium detergens6.2 ST. Multilocus sequence analysis	674:735	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	10	6	theme	unidentified	1684:1695	arg1	phospholipids					1697:1709	two unidentified phospholipids	1680:1709	two unidentified phospholipids	1680:1709	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	8	7	theme	DNA-DNA	1223:1229	arg1	relatedness					1231:1241	DNA-DNA relatedness	1223:1241	DNA-DNA relatedness	1223:1241	ANI and DNA-DNA relatedness findings strongly supported M46T as a putative novel strain of Sphingobacterium.					
30648942	11	8	theme	name	1992:1995	arg1	sp					2022:2023	the name Sphingobacterium athyrii sp	1988:2023	the name Sphingobacterium athyrii sp	1988:2023	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	4	9	theme	Sphingobacterium	637:652	arg1	CR11T					664:668	Sphingobacterium canadense CR11T	637:668	Sphingobacterium canadense CR11T	637:668	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	10	10	theme	polar	1505:1509	arg1	profile					1517:1523	The polar lipid profile	1501:1523	The polar lipid profile of strain M46T	1501:1538	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	2	11	theme	strain	282:287	arg1	presence					252:259	the presence	248:259	the presence of a novel bacterial strain named M46T	248:298	Assessment of the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching, revealed the presence of a novel bacterial strain named M46T.					
30648942	11	12	theme	Sphingobacterium	1997:2012	arg1	sp					2022:2023	the name Sphingobacterium athyrii sp	1988:2023	the name Sphingobacterium athyrii sp	1988:2023	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	4	13	theme	16S	443:445	arg1	sequences					457:465	16S rRNA gene sequences	443:465	16S rRNA gene sequences	443:465	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	11	14	theme	gene	1778:1781	arg1	similarities					1792:1803	16S rRNA gene sequence similarities	1769:1803	16S rRNA gene sequence similarities	1769:1803	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	13	15	theme	=CGMCC	2069:2074	arg1	32543T					2089:2094	=CGMCC 1.13466T=JCM 32543T	2069:2094	=CGMCC 1.13466T=JCM 32543T	2069:2094	The type strain is M46T (=CGMCC 1.13466T=JCM 32543T).					
30648942	13	15	theme	=CGMCC	2069:2074	arg1	M46T					2063:2066	M46T	2063:2066	M46T (=CGMCC 1.13466T=JCM 32543T)	2063:2095	The type strain is M46T (=CGMCC 1.13466T=JCM 32543T).					
30648942	4	16	theme	gene	452:455	arg1	sequences					457:465	16S rRNA gene sequences	443:465	16S rRNA gene sequences	443:465	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	10	17	theme	strain	1528:1533	arg1	M46T					1535:1538	strain M46T	1528:1538	strain M46T	1528:1538	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	11	18	theme	16S	1769:1771	arg1	similarities					1792:1803	16S rRNA gene sequence similarities	1769:1803	16S rRNA gene sequence similarities	1769:1803	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	4	19	theme	Phylogenetic	412:423	arg1	analysis					425:432	Phylogenetic analysis	412:432	Phylogenetic analysis based on 16S rRNA gene sequences	412:465	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	8	20	theme	putative	1281:1288	arg1	strain					1296:1301	a putative novel strain	1279:1301	a putative novel strain of Sphingobacterium	1279:1321	ANI and DNA-DNA relatedness findings strongly supported M46T as a putative novel strain of Sphingobacterium.					
30648942	8	20	theme	putative	1281:1288	arg1	M46T					1271:1274	M46T	1271:1274	M46T	1271:1274	ANI and DNA-DNA relatedness findings strongly supported M46T as a putative novel strain of Sphingobacterium.					
30648942	5	21	theme	chromosome	943:952	arg1	content					918:924	a G+C content	912:924	a G+C content of 40.6 mol% and chromosome of 6 853 865 bp	912:968	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	5	22	theme	40.6 mol	929:936	arg1	%					937:937	40.6 mol%	929:937	40.6 mol%	929:937	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	5	22	theme	40.6 mol	929:936	arg1	865 bp					963:968	6 853 865 bp	957:968	6 853 865 bp	957:968	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	4	23	theme	concatenated	753:764	arg1	sequences					766:774	concatenated sequences	753:774	concatenated sequences of the rpoB	753:786	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	3	24	with	Gram-stain-negative	308:326	arg1	cellulose					369:377	cellulose	369:377	cellulose	369:377	It was Gram-stain-negative, rod-shaped, non-motile and aerobic with cellulose and xylan degradation abilities.					
30648942	3	24	with	Gram-stain-negative	308:326	arg1	xylan					383:387	xylan	383:387	xylan	383:387	It was Gram-stain-negative, rod-shaped, non-motile and aerobic with cellulose and xylan degradation abilities.					
30648942	5	25	contain	had	908:910	arg2	content					918:924	a G+C content	912:924	a G+C content of 40.6 mol% and chromosome of 6 853 865 bp	912:968	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	5	25	contain	had	908:910	arg1	genome					886:891	The genome	882:891	The genome of strain M46T	882:906	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	11	26	theme	novel	1933:1937	arg1	species					1939:1945	a novel species	1931:1945	a novel species	1931:1945	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	5	27	theme	M46T	903:906	arg1	genome					886:891	The genome	882:891	The genome of strain M46T	882:906	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	8	28	theme	Sphingobacterium	1306:1321	arg1	strain					1296:1301	a putative novel strain	1279:1301	a putative novel strain of Sphingobacterium	1279:1321	ANI and DNA-DNA relatedness findings strongly supported M46T as a putative novel strain of Sphingobacterium.					
30648942	8	28	theme	Sphingobacterium	1306:1321	arg1	M46T					1271:1274	M46T	1271:1274	M46T	1271:1274	ANI and DNA-DNA relatedness findings strongly supported M46T as a putative novel strain of Sphingobacterium.					
30648942	3	29	dep	cellulose	369:377	arg1	abilities					401:409	degradation abilities	389:409	degradation abilities	389:409	It was Gram-stain-negative, rod-shaped, non-motile and aerobic with cellulose and xylan degradation abilities.					
30648942	7	30	theme	Sphingobacterium	1178:1193	arg1	species					1195:1201	other closely related Sphingobacterium species	1156:1201	other closely related Sphingobacterium species	1156:1201	DNA-DNA relatedness values among strain M46T and other closely related Sphingobacterium species were <70 %.					
30648942	1	31	theme	xylan-degrading	52:66	arg1	bacterium					68:76	a cellulose- and xylan-degrading bacterium	35:76	a cellulose- and xylan-degrading bacterium	35:76	nov., a cellulose- and xylan-degrading bacterium isolated from a decaying fern (Athyrium wallichianum Ching).					
30648942	1	31	theme	xylan-degrading	52:66	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., a cellulose- and xylan-degrading bacterium isolated from a decaying fern (Athyrium wallichianum Ching).					
30648942	6	32	theme	Average	971:977	arg1	identity					990:997	Average nucleotide identity	971:997	Average nucleotide identity (ANI) between strain M46T and S. detergens 6.2 ST and S. siyangense SY1T	971:1070	Average nucleotide identity (ANI) between strain M46T and S. detergens 6.2 ST and S. siyangense SY1T was 85.1 and 78.1 %, respectively.					
30648942	6	32	theme	Average	971:977	arg1	ANI					1000:1002	ANI	1000:1002	ANI	1000:1002	Average nucleotide identity (ANI) between strain M46T and S. detergens 6.2 ST and S. siyangense SY1T was 85.1 and 78.1 %, respectively.					
30648942	4	33	theme	16S	799:801	arg1	genes					808:812	16S rRNA genes	799:812	16S rRNA genes	799:812	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	9	34	theme	dominant	1472:1479	arg1	quinone					1492:1498	the dominant isoprenoid quinone	1468:1498	the dominant isoprenoid quinone	1468:1498	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	34	theme	dominant	1472:1479	arg1	MK-7					1459:1462	MK-7	1459:1462	MK-7	1459:1462	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	34	theme	dominant	1472:1479	arg1	 0					1381:1382	 0	1381:1382	 0	1381:1382	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	4	35	theme	strain	479:484	arg1	M46T					486:489	strain M46T	479:489	strain M46T	479:489	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	0	36	theme	Sphingobacterium	0:15	arg1	sp					25:26	Sphingobacterium athyrii sp	0:26	Sphingobacterium athyrii sp.	0:27	Sphingobacterium athyrii sp.					
30648942	4	37	theme	rpoB	783:786	arg1	sequences					766:774	concatenated sequences	753:774	concatenated sequences of the rpoB	753:786	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	11	38	theme	strain	1892:1897	arg1	M46T					1899:1902	strain M46T	1892:1902	strain M46T	1892:1902	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	7	39	theme	strain	1140:1145	arg1	M46T					1147:1150	strain M46T	1140:1150	strain M46T	1140:1150	DNA-DNA relatedness values among strain M46T and other closely related Sphingobacterium species were <70 %.					
30648942	9	40	theme	predominant	1328:1338	arg1	iso-C15 					1372:1379	iso-C15 	1372:1379	iso-C15 	1372:1379	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	40	theme	predominant	1328:1338	arg1	acids					1346:1350	The predominant fatty acids	1324:1350	The predominant fatty acids of strain M46T	1324:1365	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	5	41	theme	strain	896:901	arg1	M46T					903:906	strain M46T	896:906	strain M46T	896:906	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	4	42	theme	sequence	560:567	arg1	similarity					569:578	the highest sequence similarity	548:578	the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB	548:786	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	2	43	theme	diversity	167:175	arg1	Assessment					139:148	Assessment	139:148	Assessment of the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching,	139:237	Assessment of the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching, revealed the presence of a novel bacterial strain named M46T.					
30648942	8	44	theme	ANI	1215:1217	arg1	findings					1243:1250	ANI and DNA-DNA relatedness findings	1215:1250	ANI and DNA-DNA relatedness findings	1215:1250	ANI and DNA-DNA relatedness findings strongly supported M46T as a putative novel strain of Sphingobacterium.					
30648942	4	45	theme	genus	513:517	arg1	Sphingobacterium					519:534	the genus Sphingobacterium	509:534	the genus Sphingobacterium	509:534	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	11	46	theme	MLSA	1806:1809	arg1	results					1811:1817	MLSA results	1806:1817	MLSA results	1806:1817	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	7	47	theme	DNA-DNA	1107:1113	arg1	values					1127:1132	DNA-DNA relatedness values	1107:1132	DNA-DNA relatedness values among strain M46T and other closely related Sphingobacterium species	1107:1201	DNA-DNA relatedness values among strain M46T and other closely related Sphingobacterium species were <70 %.					
30648942	9	48	theme	strain	1355:1360	arg1	M46T					1362:1365	strain M46T	1355:1365	strain M46T	1355:1365	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	2	49	theme	decaying	195:202	arg1	Ching					232:236	Ching	232:236	Ching	232:236	Assessment of the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching, revealed the presence of a novel bacterial strain named M46T.					
30648942	2	49	theme	decaying	195:202	arg1	fern					204:207	a decaying fern	193:207	a decaying fern	193:207	Assessment of the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching, revealed the presence of a novel bacterial strain named M46T.					
30648942	10	50	theme	phosphoglycolipid	1625:1641	arg1	lipids					1733:1738	four unidentified lipids	1715:1738	four unidentified lipids	1715:1738	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	10	50	theme	phosphoglycolipid	1625:1641	arg1	phosphoglycolipid					1625:1641	phosphoglycolipid	1625:1641	phosphoglycolipid	1625:1641	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	10	50	theme	phosphoglycolipid	1625:1641	arg1	phospholipids					1697:1709	two unidentified phospholipids	1680:1709	two unidentified phospholipids	1680:1709	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	10	50	theme	phosphoglycolipid	1625:1641	arg1	amounts					1614:1620	minor amounts	1608:1620	minor amounts of phosphoglycolipid	1608:1641	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	11	51	theme	genus	1954:1958	arg1	Sphingobacterium					1960:1975	the genus Sphingobacterium	1950:1975	the genus Sphingobacterium	1950:1975	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	4	52	theme	sequence	719:726	arg1	analysis					728:735	Sphingobacterium detergens6.2 ST. Multilocus sequence analysis	674:735	Sphingobacterium detergens6.2 ST. Multilocus sequence analysis	674:735	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	4	53	theme	Sphingobacterium	593:608	arg1	MLSA					738:741	MLSA	738:741	MLSA	738:741	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	4	53	theme	Sphingobacterium	593:608	arg1	07T					632:634	Sphingobacterium ginsenosidimutans THG 07T	593:634	Sphingobacterium ginsenosidimutans THG 07T	593:634	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	1	54	theme	cellulose-	37:46	arg1	bacterium					68:76	a cellulose- and xylan-degrading bacterium	35:76	a cellulose- and xylan-degrading bacterium	35:76	nov., a cellulose- and xylan-degrading bacterium isolated from a decaying fern (Athyrium wallichianum Ching).					
30648942	1	54	theme	cellulose-	37:46	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., a cellulose- and xylan-degrading bacterium isolated from a decaying fern (Athyrium wallichianum Ching).					
30648942	4	55	theme	THG	628:630	arg1	MLSA					738:741	MLSA	738:741	MLSA	738:741	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	4	55	theme	THG	628:630	arg1	07T					632:634	Sphingobacterium ginsenosidimutans THG 07T	593:634	Sphingobacterium ginsenosidimutans THG 07T	593:634	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	2	56	theme	bacterial	272:280	arg1	strain					282:287	a novel bacterial strain	264:287	a novel bacterial strain named M46T	264:298	Assessment of the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching, revealed the presence of a novel bacterial strain named M46T.					
30648942	4	57	theme	%	588:588	arg1	similarity					569:578	the highest sequence similarity	548:578	the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB	548:786	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	4	58	theme	Multilocus	708:717	arg1	analysis					728:735	Sphingobacterium detergens6.2 ST. Multilocus sequence analysis	674:735	Sphingobacterium detergens6.2 ST. Multilocus sequence analysis	674:735	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	11	59	from	species	1939:1945	arg1	Sphingobacterium					1960:1975	the genus Sphingobacterium	1950:1975	the genus Sphingobacterium	1950:1975	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	4	60	theme	canadense	865:873	arg1	CR11T					875:879	S. canadense CR11T	862:879	S. canadense CR11T	862:879	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	4	61	theme	detergens6.2 ST.	691:706	arg1	analysis					728:735	Sphingobacterium detergens6.2 ST. Multilocus sequence analysis	674:735	Sphingobacterium detergens6.2 ST. Multilocus sequence analysis	674:735	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	10	62	theme	lipid	1511:1515	arg1	profile					1517:1523	The polar lipid profile	1501:1523	The polar lipid profile of strain M46T	1501:1538	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	4	63	theme	rRNA	447:450	arg1	sequences					457:465	16S rRNA gene sequences	443:465	16S rRNA gene sequences	443:465	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	8	64	theme	relatedness	1231:1241	arg1	findings					1243:1250	ANI and DNA-DNA relatedness findings	1215:1250	ANI and DNA-DNA relatedness findings	1215:1250	ANI and DNA-DNA relatedness findings strongly supported M46T as a putative novel strain of Sphingobacterium.					
30648942	4	65	theme	canadense	654:662	arg1	CR11T					664:668	Sphingobacterium canadense CR11T	637:668	Sphingobacterium canadense CR11T	637:668	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	10	66	theme	M46T	1535:1538	arg1	profile					1517:1523	The polar lipid profile	1501:1523	The polar lipid profile of strain M46T	1501:1538	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	11	67	theme	sequence	1783:1790	arg1	similarities					1792:1803	16S rRNA gene sequence similarities	1769:1803	16S rRNA gene sequence similarities	1769:1803	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	13	68	theme	1.13466T=JCM	2076:2087	arg1	32543T					2089:2094	=CGMCC 1.13466T=JCM 32543T	2069:2094	=CGMCC 1.13466T=JCM 32543T	2069:2094	The type strain is M46T (=CGMCC 1.13466T=JCM 32543T).					
30648942	13	68	theme	1.13466T=JCM	2076:2087	arg1	M46T					2063:2066	M46T	2063:2066	M46T (=CGMCC 1.13466T=JCM 32543T)	2063:2095	The type strain is M46T (=CGMCC 1.13466T=JCM 32543T).					
30648942	5	69	theme	G+C	914:916	arg1	content					918:924	a G+C content	912:924	a G+C content of 40.6 mol% and chromosome of 6 853 865 bp	912:968	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	9	70	dep	feature	1392:1398	arg1	3-OH					1449:1452	 0 3-OH	1446:1452	summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH	1385:1452	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	70	dep	feature	1392:1398	arg1	C16 					1403:1406	C16 	1403:1406	C16 	1403:1406	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	11	71	theme	rRNA	1773:1776	arg1	similarities					1792:1803	16S rRNA gene sequence similarities	1769:1803	16S rRNA gene sequence similarities	1769:1803	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	13	72	theme	type	2048:2051	arg1	strain					2053:2058	The type strain	2044:2058	The type strain	2044:2058	The type strain is M46T (=CGMCC 1.13466T=JCM 32543T).					
30648942	13	72	theme	type	2048:2051	arg1	M46T					2063:2066	M46T	2063:2066	M46T (=CGMCC 1.13466T=JCM 32543T)	2063:2095	The type strain is M46T (=CGMCC 1.13466T=JCM 32543T).					
30648942	9	73	theme	 0	1446:1447	arg1	3-OH					1449:1452	 0 3-OH	1446:1452	summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH	1385:1452	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	8	74	theme	novel	1290:1294	arg1	strain					1296:1301	a putative novel strain	1279:1301	a putative novel strain of Sphingobacterium	1279:1321	ANI and DNA-DNA relatedness findings strongly supported M46T as a putative novel strain of Sphingobacterium.					
30648942	8	74	theme	novel	1290:1294	arg1	M46T					1271:1274	M46T	1271:1274	M46T	1271:1274	ANI and DNA-DNA relatedness findings strongly supported M46T as a putative novel strain of Sphingobacterium.					
30648942	7	75	theme	related	1170:1176	arg1	species					1195:1201	other closely related Sphingobacterium species	1156:1201	other closely related Sphingobacterium species	1156:1201	DNA-DNA relatedness values among strain M46T and other closely related Sphingobacterium species were <70 %.					
30648942	10	76	theme	unidentified	1720:1731	arg1	lipids					1733:1738	four unidentified lipids	1715:1738	four unidentified lipids	1715:1738	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	6	77	theme	nucleotide	979:988	arg1	identity					990:997	Average nucleotide identity	971:997	Average nucleotide identity (ANI) between strain M46T and S. detergens 6.2 ST and S. siyangense SY1T	971:1070	Average nucleotide identity (ANI) between strain M46T and S. detergens 6.2 ST and S. siyangense SY1T was 85.1 and 78.1 %, respectively.					
30648942	6	77	theme	nucleotide	979:988	arg1	ANI					1000:1002	ANI	1000:1002	ANI	1000:1002	Average nucleotide identity (ANI) between strain M46T and S. detergens 6.2 ST and S. siyangense SY1T was 85.1 and 78.1 %, respectively.					
30648942	5	78	theme	853	959:961	arg1	865 bp					963:968	6 853 865 bp	957:968	6 853 865 bp	957:968	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	7	79	theme	other	1156:1160	arg1	species					1195:1201	other closely related Sphingobacterium species	1156:1201	other closely related Sphingobacterium species	1156:1201	DNA-DNA relatedness values among strain M46T and other closely related Sphingobacterium species were <70 %.					
30648942	5	80	theme	865 bp	963:968	arg1	chromosome					943:952	chromosome	943:952	chromosome	943:952	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	5	80	theme	865 bp	963:968	arg1	%					937:937	40.6 mol%	929:937	40.6 mol%	929:937	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	5	80	theme	865 bp	963:968	arg1	865 bp					963:968	6 853 865 bp	957:968	6 853 865 bp	957:968	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	10	81	theme	dominant	1582:1589	arg1	component					1591:1599	the dominant component	1578:1599	the dominant component	1578:1599	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	10	81	theme	dominant	1582:1589	arg1	phosphatidylethanolamine					1550:1573	phosphatidylethanolamine	1550:1573	phosphatidylethanolamine	1550:1573	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	0	82	theme	athyrii	17:23	arg1	sp					25:26	Sphingobacterium athyrii sp	0:26	Sphingobacterium athyrii sp.	0:27	Sphingobacterium athyrii sp.					
30648942	5	83	theme	%	937:937	arg1	content					918:924	a G+C content	912:924	a G+C content of 40.6 mol% and chromosome of 6 853 865 bp	912:968	The genome of strain M46T had a G+C content of 40.6 mol% and chromosome of 6 853 865 bp.					
30648942	4	84	theme	highest	552:558	arg1	similarity					569:578	the highest sequence similarity	548:578	the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB	548:786	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	11	85	theme	phenotypic	1849:1858	arg1	analyses					1882:1889	phenotypic and biochemotaxonomic analyses	1849:1889	phenotypic and biochemotaxonomic analyses	1849:1889	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30648942	1	86	theme	decaying	94:101	arg1	Ching					131:135	Ching	131:135	Ching	131:135	nov., a cellulose- and xylan-degrading bacterium isolated from a decaying fern (Athyrium wallichianum Ching).					
30648942	1	86	theme	decaying	94:101	arg1	fern					103:106	a decaying fern	92:106	a decaying fern (Athyrium wallichianum Ching)	92:136	nov., a cellulose- and xylan-degrading bacterium isolated from a decaying fern (Athyrium wallichianum Ching).					
30648942	10	87	theme	minor	1608:1612	arg1	phosphoglycolipid					1625:1641	phosphoglycolipid	1625:1641	phosphoglycolipid	1625:1641	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	10	87	theme	minor	1608:1612	arg1	amounts					1614:1620	minor amounts	1608:1620	minor amounts of phosphoglycolipid	1608:1641	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	7	88	theme	relatedness	1115:1125	arg1	values					1127:1132	DNA-DNA relatedness values	1107:1132	DNA-DNA relatedness values among strain M46T and other closely related Sphingobacterium species	1107:1201	DNA-DNA relatedness values among strain M46T and other closely related Sphingobacterium species were <70 %.					
30648942	9	89	theme	fatty	1340:1344	arg1	iso-C15 					1372:1379	iso-C15 	1372:1379	iso-C15 	1372:1379	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	89	theme	fatty	1340:1344	arg1	acids					1346:1350	The predominant fatty acids	1324:1350	The predominant fatty acids of strain M46T	1324:1365	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	2	90	theme	bacterial	157:165	arg1	diversity					167:175	the bacterial diversity	153:175	the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching,	153:237	Assessment of the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching, revealed the presence of a novel bacterial strain named M46T.					
30648942	4	91	theme	strain	826:831	arg1	M46T					833:836	strain M46T	826:836	strain M46T	826:836	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	3	92	theme	degradation	389:399	arg1	abilities					401:409	degradation abilities	389:409	degradation abilities	389:409	It was Gram-stain-negative, rod-shaped, non-motile and aerobic with cellulose and xylan degradation abilities.					
30648942	9	93	dep	C16 	1403:1406	arg1	C16 					1421:1424	C16 	1421:1424	C16 	1421:1424	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	93	dep	C16 	1403:1406	arg1	 1ω7c					1408:1412	 1ω7c	1408:1412	 1ω7c	1408:1412	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	93	dep	C16 	1403:1406	arg1	 1ω6c					1426:1430	 1ω6c	1426:1430	 1ω6c	1426:1430	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	94	theme	M46T	1362:1365	arg1	iso-C15 					1372:1379	iso-C15 	1372:1379	iso-C15 	1372:1379	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	9	94	theme	M46T	1362:1365	arg1	acids					1346:1350	The predominant fatty acids	1324:1350	The predominant fatty acids of strain M46T	1324:1365	The predominant fatty acids of strain M46T were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and iso-C17 : 0 3-OH, and MK-7 was the dominant isoprenoid quinone.					
30648942	4	95	theme	ginsenosidimutans	610:626	arg1	MLSA					738:741	MLSA	738:741	MLSA	738:741	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	4	95	theme	ginsenosidimutans	610:626	arg1	07T					632:634	Sphingobacterium ginsenosidimutans THG 07T	593:634	Sphingobacterium ginsenosidimutans THG 07T	593:634	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	4	96	theme	rRNA	803:806	arg1	genes					808:812	16S rRNA genes	799:812	16S rRNA genes	799:812	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46T was affiliated to the genus Sphingobacterium, exhibiting the highest sequence similarity of 97.9 % to Sphingobacterium ginsenosidimutans THG 07T, Sphingobacterium canadense CR11T and Sphingobacterium detergens6.2 ST. Multilocus sequence analysis (MLSA) based on concatenated sequences of the rpoB, cpn60 and 16S rRNA genes showed that strain M46T clustered together with S. canadense CR11T.					
30648942	10	97	contain	contained	1540:1548	arg1	profile					1517:1523	The polar lipid profile	1501:1523	The polar lipid profile of strain M46T	1501:1538	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	10	97	contain	contained	1540:1548	arg2	phosphatidylethanolamine					1550:1573	phosphatidylethanolamine	1550:1573	phosphatidylethanolamine	1550:1573	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	10	97	contain	contained	1540:1548	arg2	component					1591:1599	the dominant component	1578:1599	the dominant component	1578:1599	The polar lipid profile of strain M46T contained phosphatidylethanolamine as the dominant component, while minor amounts of phosphoglycolipid, one unidentified aminophospholipid, two unidentified phospholipids and four unidentified lipids were also detected.					
30648942	11	98	theme	biochemotaxonomic	1864:1880	arg1	analyses					1882:1889	phenotypic and biochemotaxonomic analyses	1849:1889	phenotypic and biochemotaxonomic analyses	1849:1889	Based on 16S rRNA gene sequence similarities, MLSA results, genomic characteristics, and phenotypic and biochemotaxonomic analyses, strain M46T is considered to represent a novel species in the genus Sphingobacterium, for which the name Sphingobacterium athyrii sp.					
30448699	7	0	theme	formed	1914:1919	arg1	tissues					1921:1927	the newly formed tissues	1904:1927	the newly formed tissues	1904:1927	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	3	1	theme	SA/agarose	706:715	arg1	hydrogel					732:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel	676:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	676:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	6	2	with	bone	1560:1563	arg1	surface					1586:1592	regular surface and highly integration	1578:1615	surface	1586:1592	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	6	2	with	bone	1560:1563	arg1	integration					1605:1615	regular surface and highly integration	1578:1615	integration	1605:1615	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	3	3	theme	/bioglass	537:545	arg1	hydrogel					562:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel	504:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	504:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	1	4	theme	host	286:289	arg1	tissue					291:296	the host tissue	282:296	the host tissue	282:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	7	5	from	bone	1813:1816	arg1	unit					1858:1861	an entire osteochondral unit	1834:1861	an entire osteochondral unit	1834:1861	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	3	6	theme	composite	722:730	arg1	hydrogel					732:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel	676:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	676:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	7	theme	composite	552:560	arg1	hydrogel					562:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel	504:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	504:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	6	8	theme	normal	1622:1627	arg1	tissues					1629:1635	normal tissues	1622:1635	normal tissues	1622:1635	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	4	9	theme	continuous	873:882	arg1	phase					887:891	The continuous SA phase	869:891	The continuous SA phase	869:891	The continuous SA phase and the stratified structure enable the scaffold to mimic the natural osteochondral structure.					
30448699	5	10	theme	alkaline	1104:1111	arg1	phosphatase					1113:1123	their alkaline phosphatase	1098:1123	their alkaline phosphatase	1098:1123	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	3	11	theme	marrow	587:592	arg1	BMSCs					606:610	BMSCs	606:610	BMSCs	606:610	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	11	theme	marrow	587:592	arg1	SA/BG + BMSCs					614:626	SA/BG + BMSCs	614:626	SA/BG + BMSCs	614:626	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	11	theme	marrow	587:592	arg1	cells					599:603	bone marrow stem cells	582:603	bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	582:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	7	12	theme	proper	1727:1732	arg1	ratio					1743:1747	its proper swelling ratio	1723:1747	its proper swelling ratio	1723:1747	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	3	13	dep	used	633:636	arg1	applied					823:829	applied	823:829	was applied for articular cartilage regeneration	819:866	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	14	theme	chondrocytes	780:791	arg1	co-culture					746:755	co-culture	746:755	co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	746:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	6	15	with	surface	1586:1592	arg1	tissues					1629:1635	normal tissues	1622:1635	normal tissues	1622:1635	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	5	16	theme	BMSCs	1076:1080	arg1	differentiation					1057:1071	the osteoblast differentiation	1042:1071	the osteoblast differentiation of BMSCs	1042:1080	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	7	17	theme	entire	1837:1842	arg1	unit					1858:1861	an entire osteochondral unit	1834:1861	an entire osteochondral unit	1834:1861	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	7	18	dep	cartilage	1787:1795	arg1	regeneration					1818:1829	regeneration	1818:1829	regeneration	1818:1829	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	5	19	theme	stratified	1335:1344	arg1	scaffold					1346:1353	this stratified scaffold	1330:1353	this stratified scaffold	1330:1353	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	7	20	theme	biomimetic	1687:1696	arg1	construct					1698:1706	This structural and functional biomimetic construct	1656:1706	This structural and functional biomimetic construct	1656:1706	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	1	21	theme	cartilage	186:194	arg1	formation					165:173	the formation	161:173	the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue	161:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	1	21	theme	cartilage	186:194	arg1	integration					232:242	the integration	228:242	the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue	161:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	1	22	theme	great	314:318	arg1	challenge					320:328	a great challenge	312:328	a great challenge	312:328	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	3	23	used	used	633:636	arg2	hydrogel					562:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel	504:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	504:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	24	theme	articular	835:843	arg1	regeneration					855:866	articular cartilage regeneration	835:866	articular cartilage regeneration	835:866	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	5	25	theme	SA/AG + ACs/BMSCs	1166:1182	arg1	hydrogel					1184:1191	the SA/AG + ACs/BMSCs hydrogel	1162:1191	the SA/AG + ACs/BMSCs hydrogel	1162:1191	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	1	26	theme	subchondral	200:210	arg1	bone					212:215	subchondral bone	200:215	subchondral bone	200:215	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	4	27	theme	osteochondral	963:975	arg1	structure					977:985	the natural osteochondral structure	951:985	the natural osteochondral structure	951:985	The continuous SA phase and the stratified structure enable the scaffold to mimic the natural osteochondral structure.					
30448699	6	28	theme	stratified	1426:1435	arg1	construct					1437:1445	the stratified construct	1422:1445	the stratified construct	1422:1445	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	7	29	theme	hyaline	1779:1785	arg1	cartilage					1787:1795	the hyaline cartilage	1775:1795	the hyaline cartilage	1775:1795	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	2	30	theme	cell	427:430	arg1	systems					432:438	multiple cell systems	418:438	multiple cell systems	418:438	In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration.					
30448699	6	31	theme	neonatal	1507:1514	arg1	tissues					1536:1542	obvious neonatal articular cartilage tissues	1499:1542	obvious neonatal articular cartilage tissues	1499:1542	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	2	32	theme	osteochondral	467:479	arg1	regeneration					481:492	osteochondral regeneration	467:492	osteochondral regeneration	467:492	In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration.					
30448699	5	33	theme	BMSCs	1242:1246	arg1	differentiation					1223:1237	the chondrocyte differentiation	1207:1237	the chondrocyte differentiation of BMSCs	1207:1246	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	5	34	theme	osteochondral	1379:1391	arg1	function					1393:1400	the natural osteochondral function	1367:1400	the natural osteochondral function	1367:1400	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	6	35	theme	defect	1485:1490	arg1	model					1492:1496	a rat osteochondral defect model	1465:1496	a rat osteochondral defect model	1465:1496	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	1	36	with	integration	232:242	arg1	tissue					291:296	the host tissue	282:296	the host tissue	282:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	5	37	theme	osteoblast	1046:1055	arg1	differentiation					1057:1071	the osteoblast differentiation	1042:1071	the osteoblast differentiation of BMSCs	1042:1080	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	6	38	dep	tissues	1536:1542	arg1	tissues					1565:1571	tissues	1565:1571	tissues	1565:1571	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	3	39	theme	subchondral	642:652	arg1	regeneration					659:670	subchondral bone regeneration	642:670	subchondral bone regeneration	642:670	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	6	40	theme	rat	1467:1469	arg1	model					1492:1496	a rat osteochondral defect model	1465:1496	a rat osteochondral defect model	1465:1496	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	3	41	theme	thermosensitive	690:704	arg1	hydrogel					732:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel	676:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	676:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	42	theme	sodium	518:523	arg1	hydrogel					562:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel	504:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	504:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	43	with	hydrogel	732:739	arg1	co-culture					746:755	co-culture	746:755	co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	746:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	5	44	theme	collagen	1279:1286	arg1	expressions					1296:1306	collagen II gene expressions	1279:1306	collagen II gene expressions	1279:1306	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	2	45	theme	stratified	394:403	arg1	scaffold					405:412	an injectable continuous stratified scaffold	369:412	an injectable continuous stratified scaffold	369:412	In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration.					
30448699	5	46	theme	gene	1291:1294	arg1	expressions					1296:1306	collagen II gene expressions	1279:1306	collagen II gene expressions	1279:1306	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	6	47	theme	osteochondral	1471:1483	arg1	model					1492:1496	a rat osteochondral defect model	1465:1496	a rat osteochondral defect model	1465:1496	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	2	48	theme	injectable	372:381	arg1	scaffold					405:412	an injectable continuous stratified scaffold	369:412	an injectable continuous stratified scaffold	369:412	In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration.					
30448699	4	49	theme	SA	884:885	arg1	phase					887:891	The continuous SA phase	869:891	The continuous SA phase	869:891	The continuous SA phase and the stratified structure enable the scaffold to mimic the natural osteochondral structure.					
30448699	6	50	theme	cartilage	1526:1534	arg1	tissues					1536:1542	obvious neonatal articular cartilage tissues	1499:1542	obvious neonatal articular cartilage tissues	1499:1542	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	6	51	with	tissues	1536:1542	arg1	surface					1586:1592	regular surface and highly integration	1578:1615	surface	1586:1592	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	6	51	with	tissues	1536:1542	arg1	integration					1605:1615	regular surface and highly integration	1578:1615	integration	1605:1615	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	1	52	theme	bone	212:215	arg1	formation					165:173	the formation	161:173	the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue	161:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	1	52	theme	bone	212:215	arg1	integration					232:242	the integration	228:242	the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue	161:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	3	53	theme	stem	594:597	arg1	BMSCs					606:610	BMSCs	606:610	BMSCs	606:610	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	53	theme	stem	594:597	arg1	SA/BG + BMSCs					614:626	SA/BG + BMSCs	614:626	SA/BG + BMSCs	614:626	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	53	theme	stem	594:597	arg1	cells					599:603	bone marrow stem cells	582:603	bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	582:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	1	54	with	regeneration	143:154	arg1	formation					165:173	the formation	161:173	the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue	161:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	1	54	with	regeneration	143:154	arg1	integration					232:242	the integration	228:242	the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue	161:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	7	55	theme	swelling	1734:1741	arg1	ratio					1743:1747	its proper swelling ratio	1723:1747	its proper swelling ratio	1723:1747	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	3	56	theme	articular	770:778	arg1	ACs					794:796	ACs	794:796	ACs	794:796	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	56	theme	articular	770:778	arg1	SA/AG + ACs/BMSCs					800:816	SA/AG + ACs/BMSCs	800:816	SA/AG + ACs/BMSCs	800:816	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	56	theme	articular	770:778	arg1	chondrocytes					780:791	articular chondrocytes	770:791	articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	770:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	57	theme	bone	582:585	arg1	BMSCs					606:610	BMSCs	606:610	BMSCs	606:610	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	57	theme	bone	582:585	arg1	SA/BG + BMSCs					614:626	SA/BG + BMSCs	614:626	SA/BG + BMSCs	614:626	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	57	theme	bone	582:585	arg1	cells					599:603	bone marrow stem cells	582:603	bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	582:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	58	theme	BMSCs	760:764	arg1	co-culture					746:755	co-culture	746:755	co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	746:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	5	59	theme	phosphatase	1113:1123	arg1	expressions					1145:1155	their alkaline phosphatase and collagen I gene expressions	1098:1155	their alkaline phosphatase and collagen I gene expressions	1098:1155	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	3	60	with	regeneration	659:670	arg1	co-culture					746:755	co-culture	746:755	co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	746:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	5	61	theme	collagen	1129:1136	arg1	expressions					1145:1155	their alkaline phosphatase and collagen I gene expressions	1098:1155	their alkaline phosphatase and collagen I gene expressions	1098:1155	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	4	62	theme	stratified	901:910	arg1	structure					912:920	the stratified structure	897:920	the stratified structure	897:920	The continuous SA phase and the stratified structure enable the scaffold to mimic the natural osteochondral structure.					
30448699	6	63	with	integration	1605:1615	arg1	tissues					1629:1635	normal tissues	1622:1635	normal tissues	1622:1635	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	7	64	theme	osteochondral	1844:1856	arg1	unit					1858:1861	an entire osteochondral unit	1834:1861	an entire osteochondral unit	1834:1861	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	7	65	theme	functional	1676:1685	arg1	construct					1698:1706	This structural and functional biomimetic construct	1656:1706	This structural and functional biomimetic construct	1656:1706	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	1	66	theme	hyaline	178:184	arg1	cartilage					186:194	hyaline cartilage	178:194	hyaline cartilage	178:194	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	7	67	theme	structural	1661:1670	arg1	construct					1698:1706	This structural and functional biomimetic construct	1656:1706	This structural and functional biomimetic construct	1656:1706	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	7	68	from	cartilage	1787:1795	arg1	unit					1858:1861	an entire osteochondral unit	1834:1861	an entire osteochondral unit	1834:1861	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	0	69	theme	osteochondral	101:113	arg1	regeneration					115:126	osteochondral regeneration	101:126	osteochondral regeneration	101:126	An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration.					
30448699	3	70	theme	cartilage	845:853	arg1	regeneration					855:866	articular cartilage regeneration	835:866	articular cartilage regeneration	835:866	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	2	71	contain	containing	358:367	arg1	construct					348:356	a construct	346:356	a construct containing an injectable continuous stratified scaffold and multiple cell systems	346:438	In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration.					
30448699	2	71	contain	containing	358:367	arg2	scaffold					405:412	an injectable continuous stratified scaffold	369:412	an injectable continuous stratified scaffold	369:412	In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration.					
30448699	2	71	contain	containing	358:367	arg2	systems					432:438	multiple cell systems	418:438	multiple cell systems	418:438	In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration.					
30448699	7	72	theme	subchondral	1801:1811	arg1	bone					1813:1816	subchondral bone	1801:1816	subchondral bone	1801:1816	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	4	73	theme	natural	955:961	arg1	structure					977:985	the natural osteochondral structure	951:985	the natural osteochondral structure	951:985	The continuous SA phase and the stratified structure enable the scaffold to mimic the natural osteochondral structure.					
30448699	5	74	theme	gene	1140:1143	arg1	expressions					1145:1155	their alkaline phosphatase and collagen I gene expressions	1098:1155	their alkaline phosphatase and collagen I gene expressions	1098:1155	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	1	75	with	formation	165:173	arg1	tissue					291:296	the host tissue	282:296	the host tissue	282:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	1	76	theme	formed	262:267	arg1	tissues					269:275	the newly formed tissues	252:275	the newly formed tissues	252:275	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	2	77	theme	multiple	418:425	arg1	systems					432:438	multiple cell systems	418:438	multiple cell systems	418:438	In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration.					
30448699	6	78	theme	articular	1516:1524	arg1	tissues					1536:1542	obvious neonatal articular cartilage tissues	1499:1542	obvious neonatal articular cartilage tissues	1499:1542	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	6	79	theme	obvious	1499:1505	arg1	tissues					1536:1542	obvious neonatal articular cartilage tissues	1499:1542	obvious neonatal articular cartilage tissues	1499:1542	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	1	80	theme	Osteochondral	129:141	arg1	regeneration					143:154	Osteochondral regeneration	129:154	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue	129:296	Osteochondral regeneration with the formation of hyaline cartilage and subchondral bone as well as the integration between the newly formed tissues with the host tissue still remains a great challenge.					
30448699	3	81	theme	bone	654:657	arg1	regeneration					659:670	subchondral bone regeneration	642:670	subchondral bone regeneration	642:670	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	5	82	theme	natural	1371:1377	arg1	function					1393:1400	the natural osteochondral function	1367:1400	the natural osteochondral function	1367:1400	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	3	83	theme	injectable	507:516	arg1	hydrogel					562:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel	504:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	504:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	0	84	theme	biomimetic	66:75	arg1	construct					77:85	structurally and functionally biomimetic construct	36:85	structurally and functionally biomimetic construct	36:85	An injectable continuous stratified structurally and functionally biomimetic construct for enhancing osteochondral regeneration.					
30448699	2	85	theme	continuous	383:392	arg1	scaffold					405:412	an injectable continuous stratified scaffold	369:412	an injectable continuous stratified scaffold	369:412	In this study, a construct containing an injectable continuous stratified scaffold and multiple cell systems was designed for enhancing osteochondral regeneration.					
30448699	3	86	contain	containing	571:580	arg1	hydrogel					562:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel	504:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	504:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	86	contain	containing	571:580	arg2	cells					599:603	bone marrow stem cells	582:603	bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	582:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	86	contain	containing	571:580	arg2	SA/BG + BMSCs					614:626	SA/BG + BMSCs	614:626	SA/BG + BMSCs	614:626	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	86	contain	containing	571:580	arg2	BMSCs					606:610	BMSCs	606:610	BMSCs	606:610	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	87	theme	injectable	679:688	arg1	hydrogel					732:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel	676:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	676:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	88	theme	SA	534:535	arg1	hydrogel					562:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel	504:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	504:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	6	89	theme	regular	1578:1584	arg1	surface					1586:1592	regular surface and highly integration	1578:1615	surface	1586:1592	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30448699	5	90	theme	chondrocyte	1211:1221	arg1	differentiation					1223:1237	the chondrocyte differentiation	1207:1237	the chondrocyte differentiation of BMSCs	1207:1246	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	5	91	theme	SA/BG + BMSCs	1005:1017	arg1	hydrogel					1019:1026	the SA/BG + BMSCs hydrogel	1001:1026	the SA/BG + BMSCs hydrogel	1001:1026	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	7	92	theme	host	1937:1940	arg1	tissue					1942:1947	the host tissue	1933:1947	the host tissue	1933:1947	This structural and functional biomimetic construct, together with its proper swelling ratio, could not only stimulate the hyaline cartilage and subchondral bone regeneration in an entire osteochondral unit but also promote the integration between the newly formed tissues and the host tissue.					
30448699	3	93	theme	alginate	525:532	arg1	hydrogel					562:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel	504:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	504:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	5	94	theme	II	1288:1289	arg1	expressions					1296:1306	collagen II gene expressions	1279:1306	collagen II gene expressions	1279:1306	In addition, the SA/BG + BMSCs hydrogel could enhance the osteoblast differentiation of BMSCs by upregulating their alkaline phosphatase and collagen I gene expressions, and the SA/AG + ACs/BMSCs hydrogel could promote the chondrocyte differentiation of BMSCs by upregulating their Acan and collagen II gene expressions, which indicated that this stratified scaffold could mimic the natural osteochondral function.					
30448699	3	95	theme	AG	718:719	arg1	hydrogel					732:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel	676:739	an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs)	676:817	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	3	96	theme	BG	548:549	arg1	hydrogel					562:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel	504:569	an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs)	504:627	Briefly, an injectable sodium alginate(SA)/bioglass (BG) composite hydrogel containing bone marrow stem cells (BMSCs) (SA/BG + BMSCs) was used for subchondral bone regeneration and an injectable thermosensitive SA/agarose (AG) composite hydrogel with co-culture of BMSCs and articular chondrocytes (ACs) (SA/AG + ACs/BMSCs) was applied for articular cartilage regeneration.					
30448699	6	97	theme	subchondral	1548:1558	arg1	bone					1560:1563	subchondral bone	1548:1563	subchondral bone	1548:1563	Furthermore, after the stratified construct was injected into a rat osteochondral defect model, obvious neonatal articular cartilage tissues and subchondral bone tissues with regular surface and highly integration with normal tissues could be observed.					
30720432	5	0	theme	2N3R	723:726	arg1	filaments					732:740	2N3R tau filaments	723:740	2N3R tau filaments	723:740	2N3R tau filaments are structurally homogeneous, and adopt a dimeric core, where the third repeats of two tau molecules pack in a parallel manner.					
30720432	7	1	theme	assembly	1140:1147	arg1	relevance					1118:1126	the relevance	1114:1126	the relevance of in vitro assembly	1114:1147	Our results illustrate the structural versatility of amyloid filaments, and raise questions about the relevance of in vitro assembly.					
30720432	5	2	theme	molecules	833:841	arg1	repeats					814:820	the third repeats	804:820	the third repeats of two tau molecules	804:841	2N3R tau filaments are structurally homogeneous, and adopt a dimeric core, where the third repeats of two tau molecules pack in a parallel manner.					
30720432	3	3	theme	cryo-	322:326	arg1	microscopy					349:358	cryo- and immuno- electron microscopy	322:358	cryo- and immuno- electron microscopy	322:358	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	3	4	used	used	317:320	arg2	we					314:315	we	314:315	we	314:315	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	1	5	theme	filamentous	156:166	arg1	inclusions					168:177	filamentous inclusions	156:177	filamentous inclusions	156:177	Assembly of microtubule-associated protein tau into filamentous inclusions underlies a range of neurodegenerative diseases.					
30720432	7	6	theme	in	1131:1132	arg1	assembly					1140:1147	in vitro assembly	1131:1147	in vitro assembly	1131:1147	Our results illustrate the structural versatility of amyloid filaments, and raise questions about the relevance of in vitro assembly.					
30720432	5	7	from	pack	843:846	arg1	manner					862:867	a parallel manner	851:867	a parallel manner	851:867	2N3R tau filaments are structurally homogeneous, and adopt a dimeric core, where the third repeats of two tau molecules pack in a parallel manner.					
30720432	5	8	theme	tau	829:831	arg1	molecules					833:841	two tau molecules	825:841	two tau molecules	825:841	2N3R tau filaments are structurally homogeneous, and adopt a dimeric core, where the third repeats of two tau molecules pack in a parallel manner.					
30720432	7	9	theme	structural	1043:1052	arg1	versatility					1054:1064	the structural versatility	1039:1064	the structural versatility of amyloid filaments	1039:1085	Our results illustrate the structural versatility of amyloid filaments, and raise questions about the relevance of in vitro assembly.					
30720432	4	10	theme	filaments	576:584	arg1	types					567:571	multiple types	558:571	multiple types of filaments	558:584	2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar 'kinked hairpin' folds, in which the second and third repeats pack against each other.					
30720432	3	11	theme	electron	340:347	arg1	microscopy					349:358	cryo- and immuno- electron microscopy	322:358	cryo- and immuno- electron microscopy	322:358	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	6	12	contain	have	962:965	arg2	cores					974:978	larger cores	967:978	larger cores	967:978	The heparin-induced tau filaments differ from those of Alzheimer's or Pick's disease, which have larger cores with different repeat compositions.					
30720432	6	12	contain	have	962:965	arg1	disease					947:953	Alzheimer's or Pick's disease	925:953	Alzheimer's or Pick's disease	925:953	The heparin-induced tau filaments differ from those of Alzheimer's or Pick's disease, which have larger cores with different repeat compositions.					
30720432	6	13	theme	larger	967:972	arg1	cores					974:978	larger cores	967:978	larger cores	967:978	The heparin-induced tau filaments differ from those of Alzheimer's or Pick's disease, which have larger cores with different repeat compositions.					
30720432	4	14	theme	kinked	637:642	arg1	hairpin					644:650	similar 'kinked hairpin' folds, in which the second and third repeats pack against each other	628:720	hairpin	644:650	2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar 'kinked hairpin' folds, in which the second and third repeats pack against each other.					
30720432	7	15	theme	filaments	1077:1085	arg1	versatility					1054:1064	the structural versatility	1039:1064	the structural versatility of amyloid filaments	1039:1085	Our results illustrate the structural versatility of amyloid filaments, and raise questions about the relevance of in vitro assembly.					
30720432	3	16	theme	heparin	525:531	arg1	presence					513:520	the presence	509:520	the presence of heparin	509:531	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	5	17	theme	third	808:812	arg1	repeats					814:820	the third repeats	804:820	the third repeats of two tau molecules	804:841	2N3R tau filaments are structurally homogeneous, and adopt a dimeric core, where the third repeats of two tau molecules pack in a parallel manner.					
30720432	0	18	theme	tau	16:18	arg1	filaments					20:28	Heparin-induced tau filaments	0:28	Heparin-induced tau filaments	0:28	Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer's and Pick's diseases.					
30720432	5	19	theme	tau	728:730	arg1	filaments					732:740	2N3R tau filaments	723:740	2N3R tau filaments	723:740	2N3R tau filaments are structurally homogeneous, and adopt a dimeric core, where the third repeats of two tau molecules pack in a parallel manner.					
30720432	6	20	theme	different	985:993	arg1	compositions					1002:1013	different repeat compositions	985:1013	different repeat compositions	985:1013	The heparin-induced tau filaments differ from those of Alzheimer's or Pick's disease, which have larger cores with different repeat compositions.					
30720432	0	21	theme	Heparin-induced	0:14	arg1	filaments					20:28	Heparin-induced tau filaments	0:28	Heparin-induced tau filaments	0:28	Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer's and Pick's diseases.					
30720432	4	22	from	pack	698:701	arg1	which					663:667	which	663:667	which	663:667	2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar 'kinked hairpin' folds, in which the second and third repeats pack against each other.					
30720432	0	23	dep	Alzheimer	71:79	arg1	diseases					94:101	diseases	94:101	diseases	94:101	Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer's and Pick's diseases.					
30720432	2	24	dep	Alzheimer	275:283	arg1	diseases					298:305	diseases	298:305	diseases	298:305	Tau filaments adopt different conformations in Alzheimer's and Pick's diseases.					
30720432	4	25	theme	types	615:619	arg1	structures					595:604	the structures	591:604	the structures of three types	591:619	2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar 'kinked hairpin' folds, in which the second and third repeats pack against each other.					
30720432	4	26	theme	multiple	558:565	arg1	types					567:571	multiple types	558:571	multiple types of filaments	558:584	2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar 'kinked hairpin' folds, in which the second and third repeats pack against each other.					
30720432	1	27	theme	neurodegenerative	200:216	arg1	diseases					218:225	neurodegenerative diseases	200:225	neurodegenerative diseases	200:225	Assembly of microtubule-associated protein tau into filamentous inclusions underlies a range of neurodegenerative diseases.					
30720432	3	28	theme	full-length	423:433	arg1	tau					441:443	recombinant full-length human tau	411:443	recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats	411:504	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	4	29	theme	similar	628:634	arg1	hairpin					644:650	similar 'kinked hairpin' folds, in which the second and third repeats pack against each other	628:720	hairpin	644:650	2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar 'kinked hairpin' folds, in which the second and third repeats pack against each other.					
30720432	2	30	theme	different	248:256	arg1	conformations					258:270	different conformations	248:270	different conformations	248:270	Tau filaments adopt different conformations in Alzheimer's and Pick's diseases.					
30720432	1	31	theme	diseases	218:225	arg1	range					191:195	a range	189:195	a range of neurodegenerative diseases	189:225	Assembly of microtubule-associated protein tau into filamentous inclusions underlies a range of neurodegenerative diseases.					
30720432	3	32	theme	human	435:439	arg1	tau					441:443	recombinant full-length human tau	411:443	recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats	411:504	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	6	33	theme	tau	890:892	arg1	filaments					894:902	The heparin-induced tau filaments	870:902	The heparin-induced tau filaments	870:902	The heparin-induced tau filaments differ from those of Alzheimer's or Pick's disease, which have larger cores with different repeat compositions.					
30720432	7	34	theme	amyloid	1069:1075	arg1	filaments					1077:1085	amyloid filaments	1069:1085	amyloid filaments	1069:1085	Our results illustrate the structural versatility of amyloid filaments, and raise questions about the relevance of in vitro assembly.					
30720432	6	35	theme	heparin-induced	874:888	arg1	filaments					894:902	The heparin-induced tau filaments	870:902	The heparin-induced tau filaments	870:902	The heparin-induced tau filaments differ from those of Alzheimer's or Pick's disease, which have larger cores with different repeat compositions.					
30720432	3	36	with	tau	441:443	arg1	four					450:453	four	450:453	four	450:453	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	3	36	with	tau	441:443	arg1	2N3R					472:475	2N3R	472:475	2N3R	472:475	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	3	36	with	tau	441:443	arg1	2N4R					456:459	2N4R	456:459	2N4R	456:459	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	3	36	with	tau	441:443	arg1	three					465:469	three	465:469	three	465:469	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	3	37	dep	four	450:453	arg1	repeats					498:504	microtubule-binding repeats	478:504	microtubule-binding repeats	478:504	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	4	38	theme	second	673:678	arg1	repeats					690:696	the second and third repeats	669:696	the second and third repeats	669:696	2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar 'kinked hairpin' folds, in which the second and third repeats pack against each other.					
30720432	3	39	theme	recombinant	411:421	arg1	tau					441:443	recombinant full-length human tau	411:443	recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats	411:504	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	2	40	theme	Tau	228:230	arg1	filaments					232:240	Tau filaments	228:240	Tau filaments	228:240	Tau filaments adopt different conformations in Alzheimer's and Pick's diseases.					
30720432	3	41	theme	microtubule-binding	478:496	arg1	repeats					498:504	microtubule-binding repeats	478:504	microtubule-binding repeats	478:504	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	7	42	dep	in	1131:1132	arg1	vitro					1134:1138	vitro	1134:1138	vitro	1134:1138	Our results illustrate the structural versatility of amyloid filaments, and raise questions about the relevance of in vitro assembly.					
30720432	4	43	theme	third	684:688	arg1	repeats					690:696	the second and third repeats	669:696	the second and third repeats	669:696	2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar 'kinked hairpin' folds, in which the second and third repeats pack against each other.					
30720432	4	44	dep	hairpin	644:650	arg1	folds					653:657	folds	653:657	folds	653:657	2N4R tau assembles into multiple types of filaments, and the structures of three types reveal similar 'kinked hairpin' folds, in which the second and third repeats pack against each other.					
30720432	1	45	theme	microtubule-associated	116:137	arg1	tau					147:149	microtubule-associated protein tau	116:149	microtubule-associated protein tau	116:149	Assembly of microtubule-associated protein tau into filamentous inclusions underlies a range of neurodegenerative diseases.					
30720432	5	46	theme	parallel	853:860	arg1	manner					862:867	a parallel manner	851:867	a parallel manner	851:867	2N3R tau filaments are structurally homogeneous, and adopt a dimeric core, where the third repeats of two tau molecules pack in a parallel manner.					
30720432	5	47	theme	dimeric	784:790	arg1	core					792:795	a dimeric core	782:795	a dimeric core	782:795	2N3R tau filaments are structurally homogeneous, and adopt a dimeric core, where the third repeats of two tau molecules pack in a parallel manner.					
30720432	1	48	theme	protein	139:145	arg1	tau					147:149	microtubule-associated protein tau	116:149	microtubule-associated protein tau	116:149	Assembly of microtubule-associated protein tau into filamentous inclusions underlies a range of neurodegenerative diseases.					
30720432	6	49	theme	repeat	995:1000	arg1	compositions					1002:1013	different repeat compositions	985:1013	different repeat compositions	985:1013	The heparin-induced tau filaments differ from those of Alzheimer's or Pick's disease, which have larger cores with different repeat compositions.					
30720432	3	50	theme	immuno-	332:338	arg1	microscopy					349:358	cryo- and immuno- electron microscopy	322:358	cryo- and immuno- electron microscopy	322:358	Here, we used cryo- and immuno- electron microscopy to characterise filaments that were assembled from recombinant full-length human tau with four (2N4R) or three (2N3R) microtubule-binding repeats in the presence of heparin.					
30720432	1	51	theme	tau	147:149	arg1	Assembly					104:111	Assembly	104:111	Assembly of microtubule-associated protein tau into filamentous inclusions	104:177	Assembly of microtubule-associated protein tau into filamentous inclusions underlies a range of neurodegenerative diseases.					
30678940	5	0	theme	bovine	802:807	arg1	BSA					824:826	BSA	824:826	BSA	824:826	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	5	0	theme	bovine	802:807	arg1	albumin					815:821	bovine serum albumin	802:821	bovine serum albumin (BSA)	802:827	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	6	1	theme	HT-22	1010:1014	arg1	neurons					1028:1034	HT-22 hippocampal neurons	1010:1034	HT-22 hippocampal neurons	1010:1034	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility.					
30678940	5	2	theme	serum	809:813	arg1	BSA					824:826	BSA	824:826	BSA	824:826	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	5	2	theme	serum	809:813	arg1	albumin					815:821	bovine serum albumin	802:821	bovine serum albumin (BSA)	802:827	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	7	3	theme	MWCNT/chitosan	1188:1201	arg1	scaffold					1203:1210	the MWCNT/chitosan scaffold	1184:1210	the MWCNT/chitosan scaffold	1184:1210	The neurons acquire elongated geometry on the MWCNT/chitosan scaffold, while GNP reinforcement drives the neurons to spread cellular processes radially.					
30678940	2	4	from	effect	328:333	arg1	regeneration					401:412	neural cell regeneration	389:412	neural cell regeneration	389:412	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	2	4	from	effect	328:333	arg1	morphology					356:365	morphology	356:365	morphology of these materials	356:384	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	2	5	theme	difference	342:351	arg1	effect					328:333	the effect	324:333	the effect of the difference in morphology of these materials on neural cell regeneration	324:412	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	6	6	theme	neurons	1028:1034	arg1	Interactions					994:1005	Interactions	994:1005	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices	994:1115	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility.					
30678940	0	7	theme	polymeric	99:107	arg1	scaffolds					109:117	polymeric scaffolds	99:117	polymeric scaffolds	99:117	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	3	8	theme	MWCNT/chitosan	532:545	arg1	highest-conductivity					557:576	highest-conductivity	557:576	highest-conductivity	557:576	Electrical conductivities of all the hybrid scaffolds are found to be in between that of MWCNT/chitosan scaffold (highest-conductivity) and GNP/chitosan scaffold (lowest-conductivity).					
30678940	3	8	theme	MWCNT/chitosan	532:545	arg1	scaffold					547:554	MWCNT/chitosan scaffold	532:554	MWCNT/chitosan scaffold (highest-conductivity)	532:577	Electrical conductivities of all the hybrid scaffolds are found to be in between that of MWCNT/chitosan scaffold (highest-conductivity) and GNP/chitosan scaffold (lowest-conductivity).					
30678940	1	9	theme	large	252:256	arg1	number					258:263	a large number	250:263	a large number of biomedical applications	250:290	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	4	10	theme	GNP/chitosan	742:753	arg1	scaffolds					755:763	MWCNT/chitosan and GNP/chitosan scaffolds	723:763	MWCNT/chitosan and GNP/chitosan scaffolds	723:763	While, hybrid scaffolds show improvement in elastic modulus and ultimate tensile strength over MWCNT/chitosan and GNP/chitosan scaffolds.					
30678940	5	11	theme	protein	770:776	arg1	isotherms					789:797	The protein adsorption isotherms	766:797	The protein adsorption isotherms of bovine serum albumin (BSA)	766:827	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	0	12	from	outgrowth	46:54	arg1	graphene					79:86	graphene	79:86	graphene	79:86	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	0	12	from	outgrowth	46:54	arg1	nanotube					66:73	carbon nanotube	59:73	carbon nanotube	59:73	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	6	13	theme	hybrid	1092:1097	arg1	matrices					1108:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility.					
30678940	2	14	theme	neural	389:394	arg1	regeneration					401:412	neural cell regeneration	389:412	neural cell regeneration	389:412	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	5	15	theme	adsorption	778:787	arg1	isotherms					789:797	The protein adsorption isotherms	766:797	The protein adsorption isotherms of bovine serum albumin (BSA)	766:827	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	5	16	theme	albumin	815:821	arg1	isotherms					789:797	The protein adsorption isotherms	766:797	The protein adsorption isotherms of bovine serum albumin (BSA)	766:827	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	5	17	theme	greater	834:840	arg1	Keq					864:866	Keq	864:866	Keq	864:866	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	5	17	theme	greater	834:840	arg1	constant					854:861	greater equilibrium constant	834:861	greater equilibrium constant (Keq)	834:867	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	3	18	theme	Electrical	443:452	arg1	conductivities					454:467	Electrical conductivities	443:467	Electrical conductivities of all the hybrid scaffolds	443:495	Electrical conductivities of all the hybrid scaffolds are found to be in between that of MWCNT/chitosan scaffold (highest-conductivity) and GNP/chitosan scaffold (lowest-conductivity).					
30678940	0	19	theme	neural	13:18	arg1	adhesion					25:32	Differential neural cell adhesion	0:32	Differential neural cell adhesion	0:32	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	2	20	from	difference	342:351	arg1	morphology					356:365	morphology	356:365	morphology of these materials	356:384	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	5	21	theme	cell	965:968	arg1	adhesion					970:977	cell adhesion	965:977	cell adhesion	965:977	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	2	22	theme	materials	376:384	arg1	morphology					356:365	morphology	356:365	morphology of these materials	356:384	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	0	23	theme	Differential	0:11	arg1	adhesion					25:32	Differential neural cell adhesion	0:32	Differential neural cell adhesion	0:32	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	5	24	theme	GNP/chitosan	872:883	arg1	composites					885:894	GNP/chitosan composites	872:894	GNP/chitosan composites as compared to MWCNT/chitosan composites	872:935	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	4	25	from	improvement	657:667	arg1	strength					709:716	ultimate tensile strength	692:716	ultimate tensile strength	692:716	While, hybrid scaffolds show improvement in elastic modulus and ultimate tensile strength over MWCNT/chitosan and GNP/chitosan scaffolds.					
30678940	4	25	from	improvement	657:667	arg1	modulus					680:686	elastic modulus	672:686	elastic modulus	672:686	While, hybrid scaffolds show improvement in elastic modulus and ultimate tensile strength over MWCNT/chitosan and GNP/chitosan scaffolds.					
30678940	1	26	theme	multiwalled	184:194	arg1	nanotubes					203:211	multiwalled carbon nanotubes	184:211	multiwalled carbon nanotubes (MWCNTs)	184:220	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	26	theme	multiwalled	184:194	arg1	MWCNTs					214:219	MWCNTs	214:219	MWCNTs	214:219	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	5	27	theme	equilibrium	842:852	arg1	Keq					864:866	Keq	864:866	Keq	864:866	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	5	27	theme	equilibrium	842:852	arg1	constant					854:861	greater equilibrium constant	834:861	greater equilibrium constant (Keq)	834:867	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	1	28	theme	biomedical	268:277	arg1	applications					279:290	biomedical applications	268:290	biomedical applications	268:290	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	2	29	from	morphology	356:365	arg1	effect					328:333	the effect	324:333	the effect of the difference in morphology of these materials on neural cell regeneration	324:412	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	2	30	theme	present	297:303	arg1	study					305:309	The present study	293:309	The present study	293:309	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	7	31	theme	elongated	1162:1170	arg1	geometry					1172:1179	elongated geometry	1162:1179	elongated geometry	1162:1179	The neurons acquire elongated geometry on the MWCNT/chitosan scaffold, while GNP reinforcement drives the neurons to spread cellular processes radially.					
30678940	1	32	theme	carbon	196:201	arg1	nanotubes					203:211	multiwalled carbon nanotubes	184:211	multiwalled carbon nanotubes (MWCNTs)	184:220	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	32	theme	carbon	196:201	arg1	MWCNTs					214:219	MWCNTs	214:219	MWCNTs	214:219	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	2	33	theme	biodegradable	419:431	arg1	scaffold					433:440	a biodegradable scaffold	417:440	a biodegradable scaffold	417:440	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	1	34	theme	applications	279:290	arg1	number					258:263	a large number	250:263	a large number of biomedical applications	250:290	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	4	35	theme	hybrid	635:640	arg1	scaffolds					642:650	hybrid scaffolds	635:650	hybrid scaffolds	635:650	While, hybrid scaffolds show improvement in elastic modulus and ultimate tensile strength over MWCNT/chitosan and GNP/chitosan scaffolds.					
30678940	0	36	theme	neurite	38:44	arg1	outgrowth					46:54	neurite outgrowth	38:54	neurite outgrowth	38:54	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	5	37	theme	more	946:949	arg1	potential					951:959	more potential	946:959	more potential for cell adhesion	946:977	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	6	38	theme	MWCNT/GNP	1082:1090	arg1	matrices					1108:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility.					
30678940	4	39	theme	elastic	672:678	arg1	modulus					680:686	elastic modulus	672:686	elastic modulus	672:686	While, hybrid scaffolds show improvement in elastic modulus and ultimate tensile strength over MWCNT/chitosan and GNP/chitosan scaffolds.					
30678940	7	40	theme	GNP	1219:1221	arg1	reinforcement					1223:1235	GNP reinforcement	1219:1235	GNP reinforcement	1219:1235	The neurons acquire elongated geometry on the MWCNT/chitosan scaffold, while GNP reinforcement drives the neurons to spread cellular processes radially.					
30678940	2	41	theme	cell	396:399	arg1	regeneration					401:412	neural cell regeneration	389:412	neural cell regeneration	389:412	The present study investigates the effect of the difference in morphology of these materials on neural cell regeneration on a biodegradable scaffold.					
30678940	7	42	theme	cellular	1266:1273	arg1	processes					1275:1283	cellular processes	1266:1283	cellular processes	1266:1283	The neurons acquire elongated geometry on the MWCNT/chitosan scaffold, while GNP reinforcement drives the neurons to spread cellular processes radially.					
30678940	6	43	theme	various	1074:1080	arg1	matrices					1108:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility.					
30678940	3	44	theme	GNP/chitosan	583:594	arg1	lowest-conductivity					606:624	lowest-conductivity	606:624	lowest-conductivity	606:624	Electrical conductivities of all the hybrid scaffolds are found to be in between that of MWCNT/chitosan scaffold (highest-conductivity) and GNP/chitosan scaffold (lowest-conductivity).					
30678940	3	44	theme	GNP/chitosan	583:594	arg1	scaffold					596:603	GNP/chitosan scaffold	583:603	GNP/chitosan scaffold (lowest-conductivity)	583:625	Electrical conductivities of all the hybrid scaffolds are found to be in between that of MWCNT/chitosan scaffold (highest-conductivity) and GNP/chitosan scaffold (lowest-conductivity).					
30678940	1	45	theme	Carbon	120:125	arg1	nanotubes					203:211	multiwalled carbon nanotubes	184:211	multiwalled carbon nanotubes (MWCNTs)	184:220	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	45	theme	Carbon	120:125	arg1	candidates					236:245	potential candidates	226:245	potential candidates in a large number of biomedical applications	226:290	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	45	theme	Carbon	120:125	arg1	nanoplatelets					159:171	graphene nanoplatelets	150:171	graphene nanoplatelets (GNPs)	150:178	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	45	theme	Carbon	120:125	arg1	nanomaterials					127:139	Carbon nanomaterials	120:139	Carbon nanomaterials	120:139	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	46	from	candidates	236:245	arg1	number					258:263	a large number	250:263	a large number of biomedical applications	250:290	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	5	47	dep	composites	885:894	arg1	compared					899:906	compared	899:906	compared to MWCNT/chitosan composites	899:935	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	4	48	theme	MWCNT/chitosan	723:736	arg1	scaffolds					755:763	MWCNT/chitosan and GNP/chitosan scaffolds	723:763	MWCNT/chitosan and GNP/chitosan scaffolds	723:763	While, hybrid scaffolds show improvement in elastic modulus and ultimate tensile strength over MWCNT/chitosan and GNP/chitosan scaffolds.					
30678940	4	49	theme	ultimate	692:699	arg1	strength					709:716	ultimate tensile strength	692:716	ultimate tensile strength	692:716	While, hybrid scaffolds show improvement in elastic modulus and ultimate tensile strength over MWCNT/chitosan and GNP/chitosan scaffolds.					
30678940	3	50	theme	hybrid	480:485	arg1	scaffolds					487:495	all the hybrid scaffolds	472:495	all the hybrid scaffolds	472:495	Electrical conductivities of all the hybrid scaffolds are found to be in between that of MWCNT/chitosan scaffold (highest-conductivity) and GNP/chitosan scaffold (lowest-conductivity).					
30678940	6	51	theme	hippocampal	1016:1026	arg1	neurons					1028:1034	HT-22 hippocampal neurons	1010:1034	HT-22 hippocampal neurons	1010:1034	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility.					
30678940	6	52	with	Interactions	994:1005	arg1	GNP/chitosan					1057:1068	GNP/chitosan	1057:1068	GNP/chitosan	1057:1068	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility.					
30678940	6	52	with	Interactions	994:1005	arg1	matrices					1108:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility.					
30678940	3	53	theme	scaffolds	487:495	arg1	conductivities					454:467	Electrical conductivities	443:467	Electrical conductivities of all the hybrid scaffolds	443:495	Electrical conductivities of all the hybrid scaffolds are found to be in between that of MWCNT/chitosan scaffold (highest-conductivity) and GNP/chitosan scaffold (lowest-conductivity).					
30678940	5	54	theme	MWCNT/chitosan	911:924	arg1	composites					926:935	MWCNT/chitosan composites	911:935	MWCNT/chitosan composites	911:935	The protein adsorption isotherms of bovine serum albumin (BSA) show greater equilibrium constant (Keq) on GNP/chitosan composites as compared to MWCNT/chitosan composites, proving more potential for cell adhesion in the former.					
30678940	6	55	theme	chitosan	1099:1106	arg1	matrices					1108:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	various MWCNT/GNP hybrid chitosan matrices	1074:1115	Interactions of HT-22 hippocampal neurons with MWCNT/chitosan, GNP/chitosan and various MWCNT/GNP hybrid chitosan matrices prove cytocompatibility.					
30678940	4	56	theme	tensile	701:707	arg1	strength					709:716	ultimate tensile strength	692:716	ultimate tensile strength	692:716	While, hybrid scaffolds show improvement in elastic modulus and ultimate tensile strength over MWCNT/chitosan and GNP/chitosan scaffolds.					
30678940	1	57	theme	potential	226:234	arg1	nanotubes					203:211	multiwalled carbon nanotubes	184:211	multiwalled carbon nanotubes (MWCNTs)	184:220	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	57	theme	potential	226:234	arg1	candidates					236:245	potential candidates	226:245	potential candidates in a large number of biomedical applications	226:290	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	57	theme	potential	226:234	arg1	nanoplatelets					159:171	graphene nanoplatelets	150:171	graphene nanoplatelets (GNPs)	150:178	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	57	theme	potential	226:234	arg1	nanomaterials					127:139	Carbon nanomaterials	120:139	Carbon nanomaterials	120:139	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	0	58	theme	carbon	59:64	arg1	nanotube					66:73	carbon nanotube	59:73	carbon nanotube	59:73	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	0	59	from	adhesion	25:32	arg1	graphene					79:86	graphene	79:86	graphene	79:86	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	0	59	from	adhesion	25:32	arg1	nanotube					66:73	carbon nanotube	59:73	carbon nanotube	59:73	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	0	60	theme	cell	20:23	arg1	adhesion					25:32	Differential neural cell adhesion	0:32	Differential neural cell adhesion	0:32	Differential neural cell adhesion and neurite outgrowth on carbon nanotube and graphene reinforced polymeric scaffolds.					
30678940	1	61	theme	graphene	150:157	arg1	GNPs					174:177	GNPs	174:177	GNPs	174:177	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
30678940	1	61	theme	graphene	150:157	arg1	nanoplatelets					159:171	graphene nanoplatelets	150:171	graphene nanoplatelets (GNPs)	150:178	Carbon nanomaterials, such as graphene nanoplatelets (GNPs) and multiwalled carbon nanotubes (MWCNTs) are potential candidates in a large number of biomedical applications.					
31170486	2	0	theme	starch	466:471	arg1	retrogradation					473:486	starch retrogradation	466:486	starch retrogradation	466:486	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	2	1	theme	starch	344:349	arg1	plasticizers					351:362	the best starch plasticizers	335:362	the best starch plasticizers	335:362	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	4	2	from	structure	652:660	arg1	terms					665:669	terms	665:669	terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw)	665:762	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	0	3	from	study	12:16	arg1	properties					21:30	properties	21:30	properties of starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer	21:140	Comparative study on properties of starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer.					
31170486	6	4	theme	monoester	1199:1207	arg1	content					1209:1215	lower phosphate monoester content	1183:1215	lower phosphate monoester content	1183:1215	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	6	5	theme	corn	1040:1043	arg1	starches					1055:1062	corn and wheat starches	1040:1062	corn and wheat starches	1040:1062	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	2	6	theme	best	339:342	arg1	plasticizers					351:362	the best starch plasticizers	335:362	the best starch plasticizers	335:362	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	8	7	theme	starch	1372:1377	arg1	films					1379:1383	starch films	1372:1383	starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene	1372:1476	[emim+][Ac-] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.					
31170486	6	8	theme	phosphate	1189:1197	arg1	content					1209:1215	lower phosphate monoester content	1183:1215	lower phosphate monoester content	1183:1215	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	7	9	theme	mechanical	1291:1300	arg1	properties					1302:1311	their better mechanical properties	1278:1311	their better mechanical properties	1278:1311	This more crystalline structure could be the responsible of their better mechanical properties.					
31170486	5	10	theme	potato	902:907	arg1	starch					909:914	potato starch	902:914	potato starch	902:914	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	5	11	dep	amorphous	832:840	arg1	contributing					922:933	contributing	922:933	contributing to a lower rearrangement of the starch chains making the crystallization process difficult	922:1024	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	5	12	theme	phosphate	871:879	arg1	content					891:897	the higher Mw and phosphate monoester content	853:897	content	891:897	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	7	13	theme	more	1223:1226	arg1	responsible					1263:1273	responsible	1263:1273	responsible	1263:1273	This more crystalline structure could be the responsible of their better mechanical properties.					
31170486	7	13	theme	more	1223:1226	arg1	structure					1240:1248	This more crystalline structure	1218:1248	This more crystalline structure	1218:1248	This more crystalline structure could be the responsible of their better mechanical properties.					
31170486	5	14	theme	starch	909:914	arg1	content					891:897	the higher Mw and phosphate monoester content	853:897	content	891:897	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	5	14	theme	starch	909:914	arg1	Mw					864:865	the higher Mw and phosphate monoester content	853:897	Mw	864:865	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	6	15	theme	lower	1183:1187	arg1	content					1209:1215	lower phosphate monoester content	1183:1215	lower phosphate monoester content	1183:1215	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	4	16	theme	monoester	718:726	arg1	contents					728:735	phosphate monoester contents	708:735	phosphate monoester contents	708:735	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	4	17	theme	granular	674:681	arg1	composition					683:693	granular composition	674:693	granular composition (amylose and phosphate monoester contents)	674:736	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	2	18	theme	film	503:506	arg1	stability					508:516	film stability	503:516	film stability	503:516	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	8	19	theme	synthetic	1443:1451	arg1	polyethylene					1465:1476	synthetic low-density polyethylene	1443:1476	synthetic low-density polyethylene	1443:1476	[emim+][Ac-] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.					
31170486	7	20	theme	crystalline	1228:1238	arg1	responsible					1263:1273	responsible	1263:1273	responsible	1263:1273	This more crystalline structure could be the responsible of their better mechanical properties.					
31170486	7	20	theme	crystalline	1228:1238	arg1	structure					1240:1248	This more crystalline structure	1218:1248	This more crystalline structure	1218:1248	This more crystalline structure could be the responsible of their better mechanical properties.					
31170486	4	21	from	effect	625:630	arg1	terms					665:669	terms	665:669	terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw)	665:762	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	4	21	from	effect	625:630	arg1	properties					772:781	film properties	767:781	film properties	767:781	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	2	22	theme	great	378:382	arg1	capacity					384:391	their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability	372:516	their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability	372:516	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	8	23	theme	wheat	1402:1406	arg1	films					1415:1419	wheat starch films	1402:1419	wheat starch films	1402:1419	[emim+][Ac-] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.					
31170486	8	24	theme	low-density	1453:1463	arg1	polyethylene					1465:1476	synthetic low-density polyethylene	1443:1476	synthetic low-density polyethylene	1443:1476	[emim+][Ac-] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.					
31170486	0	25	theme	Comparative	0:10	arg1	study					12:16	Comparative study	0:16	Comparative study on properties of starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer.	0:141	Comparative study on properties of starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer.					
31170486	0	26	theme	1-ethyl-3-methylimidazolium	91:117	arg1	acetate					119:125	1-ethyl-3-methylimidazolium acetate	91:125	1-ethyl-3-methylimidazolium acetate	91:125	Comparative study on properties of starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer.					
31170486	4	27	dep	composition	683:693	arg1	contents					728:735	phosphate monoester contents	708:735	phosphate monoester contents	708:735	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	4	27	dep	composition	683:693	arg1	amylose					696:702	amylose	696:702	amylose	696:702	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	2	28	theme	starch	421:426	arg1	ability					444:450	starch and hence great ability	421:450	starch and hence great ability of preventing starch retrogradation and increasing film stability	421:516	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	6	29	theme	growth	1166:1171	arg1	rate					1173:1176	higher mobility and crystal growth rate	1138:1176	rate	1173:1176	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	5	30	theme	monoester	881:889	arg1	content					891:897	the higher Mw and phosphate monoester content	853:897	content	891:897	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	2	31	theme	due	365:367	arg1	plasticizers					351:362	the best starch plasticizers	335:362	the best starch plasticizers	335:362	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	4	32	theme	film	767:770	arg1	properties					772:781	film properties	767:781	film properties	767:781	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	4	33	theme	phosphate	708:716	arg1	contents					728:735	phosphate monoester contents	708:735	phosphate monoester contents	708:735	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	2	34	theme	hydrogen	401:408	arg1	bonds					410:414	hydrogen bonds	401:414	hydrogen bonds	401:414	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	6	35	theme	wheat	1049:1053	arg1	starches					1055:1062	corn and wheat starches	1040:1062	corn and wheat starches	1040:1062	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	8	36	theme	simple	1495:1500	arg1	process					1531:1537	a simple and environmentally-friendly process	1493:1537	a simple and environmentally-friendly process	1493:1537	[emim+][Ac-] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.					
31170486	3	37	theme	wheat	607:611	arg1	starch					613:618	wheat starch	607:618	wheat starch	607:618	In this work, [emim+][Ac-]-plasticized starch films were prepared from potato, corn and wheat starch.					
31170486	4	38	theme	structure	652:660	arg1	effect					625:630	The effect	621:630	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties	621:781	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	4	39	theme	molecular	642:650	arg1	structure					652:660	starch molecular structure	635:660	starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw)	635:762	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	0	40	theme	starch	35:40	arg1	films					42:46	starch films	35:46	starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer	35:140	Comparative study on properties of starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer.					
31170486	8	41	dep	corn	1393:1396	arg1	properties					1429:1438	similar properties	1421:1438	similar properties	1421:1438	[emim+][Ac-] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.					
31170486	5	42	theme	difficult	1016:1024	arg1	process					1008:1014	the crystallization process	988:1014	the crystallization process difficult	988:1024	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	6	43	theme	crystal	1158:1164	arg1	rate					1173:1176	higher mobility and crystal growth rate	1138:1176	rate	1173:1176	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	1	44	theme	due	215:217	arg1	polymers					206:213	synthetic polymers	196:213	synthetic polymers due to their biodegradability and low cost	196:256	Starch films are gaining attention as substitutes of synthetic polymers due to their biodegradability and low cost.					
31170486	5	45	theme	starch	805:810	arg1	films					812:816	Potato starch films	798:816	Potato starch films	798:816	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	5	45	theme	starch	805:810	arg1	amorphous					832:840	amorphous	832:840	amorphous	832:840	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	8	46	theme	environmentally-friendly	1506:1529	arg1	process					1531:1537	a simple and environmentally-friendly process	1493:1537	a simple and environmentally-friendly process	1493:1537	[emim+][Ac-] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.					
31170486	7	47	theme	properties	1302:1311	arg1	responsible					1263:1273	responsible	1263:1273	responsible	1263:1273	This more crystalline structure could be the responsible of their better mechanical properties.					
31170486	7	47	theme	properties	1302:1311	arg1	structure					1240:1248	This more crystalline structure	1218:1248	This more crystalline structure	1218:1248	This more crystalline structure could be the responsible of their better mechanical properties.					
31170486	5	48	theme	Potato	798:803	arg1	films					812:816	Potato starch films	798:816	Potato starch films	798:816	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	5	48	theme	Potato	798:803	arg1	amorphous					832:840	amorphous	832:840	amorphous	832:840	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	2	49	theme	great	438:442	arg1	ability					444:450	starch and hence great ability	421:450	starch and hence great ability of preventing starch retrogradation and increasing film stability	421:516	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	3	50	theme	-plasticized	545:556	arg1	films					565:569	[emim+][Ac-]-plasticized starch films	533:569	[emim+][Ac-]-plasticized starch films	533:569	In this work, [emim+][Ac-]-plasticized starch films were prepared from potato, corn and wheat starch.					
31170486	0	51	theme	films	42:46	arg1	properties					21:30	properties	21:30	properties of starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer	21:140	Comparative study on properties of starch films obtained from potato, corn and wheat using 1-ethyl-3-methylimidazolium acetate as plasticizer.					
31170486	6	52	theme	lower	1112:1116	arg1	Mw					1118:1119	their lower Mw	1106:1119	their lower Mw	1106:1119	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	5	53	theme	chains	974:979	arg1	rearrangement					946:958	a lower rearrangement	938:958	a lower rearrangement of the starch chains making the crystallization process difficult	938:1024	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	3	54	theme	starch	558:563	arg1	films					565:569	[emim+][Ac-]-plasticized starch films	533:569	[emim+][Ac-]-plasticized starch films	533:569	In this work, [emim+][Ac-]-plasticized starch films were prepared from potato, corn and wheat starch.					
31170486	5	55	theme	higher	857:862	arg1	Mw					864:865	the higher Mw and phosphate monoester content	853:897	Mw	864:865	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	6	56	theme	higher	1138:1143	arg1	mobility					1145:1152	higher mobility and crystal growth rate	1138:1176	mobility	1145:1152	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	4	57	theme	weight	752:757	arg1	terms					665:669	terms	665:669	terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw)	665:762	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	2	58	theme	ionic	264:268	arg1	alternatives					302:313	alternatives	302:313	alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability	302:516	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	2	58	theme	ionic	264:268	arg1	liquids					270:276	Some ionic liquids	259:276	Some ionic liquids	259:276	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	4	59	theme	composition	683:693	arg1	terms					665:669	terms	665:669	terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw)	665:762	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	6	60	theme	crystalline	1077:1087	arg1	films					1089:1093	more crystalline films	1072:1093	more crystalline films	1072:1093	In contrast, corn and wheat starches lead to more crystalline films because of their lower Mw, which may imply higher mobility and crystal growth rate, and lower phosphate monoester content.					
31170486	8	61	theme	starch	1408:1413	arg1	films					1415:1419	wheat starch films	1402:1419	wheat starch films	1402:1419	[emim+][Ac-] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.					
31170486	4	62	theme	molecular	742:750	arg1	Mw					760:761	Mw	760:761	Mw	760:761	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	4	62	theme	molecular	742:750	arg1	weight					752:757	molecular weight	742:757	molecular weight (Mw)	742:762	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	4	63	theme	starch	635:640	arg1	structure					652:660	starch molecular structure	635:660	starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw)	635:762	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	5	64	theme	starch	967:972	arg1	chains					974:979	the starch chains	963:979	the starch chains making the crystallization process difficult	963:1024	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	5	65	theme	crystallization	992:1006	arg1	process					1008:1014	the crystallization process	988:1014	the crystallization process difficult	988:1024	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	1	66	theme	synthetic	196:204	arg1	polymers					206:213	synthetic polymers	196:213	synthetic polymers due to their biodegradability and low cost	196:256	Starch films are gaining attention as substitutes of synthetic polymers due to their biodegradability and low cost.					
31170486	1	67	theme	Starch	143:148	arg1	films					150:154	Starch films	143:154	Starch films	143:154	Starch films are gaining attention as substitutes of synthetic polymers due to their biodegradability and low cost.					
31170486	4	68	from	terms	665:669	arg1	effect					625:630	The effect	621:630	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties	621:781	The effect of starch molecular structure in terms of granular composition (amylose and phosphate monoester contents) and molecular weight (Mw) on film properties was evaluated.					
31170486	8	69	theme	similar	1421:1427	arg1	properties					1429:1438	similar properties	1421:1438	similar properties	1421:1438	[emim+][Ac-] can be considered suitable for manufacturing starch films showing corn and wheat starch films similar properties to synthetic low-density polyethylene, but involving a simple and environmentally-friendly process.					
31170486	5	70	theme	lower	940:944	arg1	rearrangement					946:958	a lower rearrangement	938:958	a lower rearrangement of the starch chains making the crystallization process difficult	938:1024	Potato starch films were the most amorphous because of the higher Mw and phosphate monoester content of potato starch, both contributing to a lower rearrangement of the starch chains making the crystallization process difficult.					
31170486	2	71	theme	plasticizers	351:362	arg1	plasticizers					351:362	the best starch plasticizers	335:362	the best starch plasticizers	335:362	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	2	71	theme	plasticizers	351:362	arg1	one					328:330	one	328:330	one	328:330	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	2	71	theme	plasticizers	351:362	arg1	glycerol					318:325	glycerol	318:325	glycerol	318:325	Some ionic liquids have been postulated as alternatives to glycerol, one of the best starch plasticizers, due to their great capacity to form hydrogen bonds with starch and hence great ability of preventing starch retrogradation and increasing film stability.					
31170486	1	72	theme	low	249:251	arg1	cost					253:256	low cost	249:256	low cost	249:256	Starch films are gaining attention as substitutes of synthetic polymers due to their biodegradability and low cost.					
31169487	2	0	theme	taxonomic	104:112	arg1	position					114:121	The taxonomic position	100:121	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T,	100:242	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	11	1	theme	phenotypic	1344:1353	arg1	characteristics					1355:1369	phenotypic characteristics	1344:1369	phenotypic characteristics	1344:1369	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	5	2	theme	cell	578:581	arg1	hydrolysates					583:594	the whole cell hydrolysates	568:594	the whole cell hydrolysates	568:594	Galactose, arabinose and ribose were detected as the sugar compositions in the whole cell hydrolysates.					
31169487	11	3	theme	family	1455:1460	arg1	Propionibacteriaceae					1462:1481	the family Propionibacteriaceae	1451:1481	the family Propionibacteriaceae	1451:1481	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	11	4	dep	name	1498:1501	arg1	nov.					1536:1539	Desertihabitans aurantiacus gen. nov.	1503:1539	the name Desertihabitans aurantiacus gen. nov.	1494:1539	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	4	5	theme	%	452:452	arg1	concentration					459:471	0-1 % NaCl concentration	448:471	0-1 % NaCl concentration (range 0-5 %, w/v)	448:490	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	12	6	theme	CPCC	1569:1572	arg1	204711T					1574:1580	CPCC 204711T	1569:1580	CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain	1569:1626	nov. is proposed, with CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain.					
31169487	12	6	theme	CPCC	1569:1572	arg1	105431T					1600:1606	=KCTC 39977T=DSM 105431T	1583:1606	=KCTC 39977T=DSM 105431T	1583:1606	nov. is proposed, with CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain.					
31169487	11	7	theme	genus	1442:1446	arg1	species					1425:1431	a novel species	1417:1431	a novel species	1417:1431	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	10	8	theme	Mariniluteicoccus	1271:1287	arg1	2617T					1306:1310	Mariniluteicoccus endophyticus YIM 2617T	1271:1310	Mariniluteicoccus endophyticus YIM 2617T	1271:1310	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	11	9	theme	strain	1386:1391	arg1	204711T					1398:1404	strain CPCC 204711T	1386:1404	strain CPCC 204711T	1386:1404	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	4	10	theme	0-1 	448:451	arg1	%					452:452	%	452:452	%	452:452	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	12	11	theme	=KCTC	1583:1587	arg1	204711T					1574:1580	CPCC 204711T	1569:1580	CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain	1569:1626	nov. is proposed, with CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain.					
31169487	12	11	theme	=KCTC	1583:1587	arg1	105431T					1600:1606	=KCTC 39977T=DSM 105431T	1583:1606	=KCTC 39977T=DSM 105431T	1583:1606	nov. is proposed, with CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain.					
31169487	8	12	theme	fatty	900:904	arg1	 0					928:929	 0	928:929	 0	928:929	The predominant fatty acid was anteiso-C15 : 0.					
31169487	8	12	theme	fatty	900:904	arg1	acid					906:909	The predominant fatty acid	884:909	The predominant fatty acid	884:909	The predominant fatty acid was anteiso-C15 : 0.					
31169487	11	13	from	genus	1442:1446	arg1	Propionibacteriaceae					1462:1481	the family Propionibacteriaceae	1451:1481	the family Propionibacteriaceae	1451:1481	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	2	14	theme	polyphasic	268:277	arg1	approach					279:286	a polyphasic approach	266:286	a polyphasic approach	266:286	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	4	15	theme	range	426:430	arg1	pH					432:433	range pH 6.0-8.0	426:441	range pH 6.0-8.0	426:441	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	4	15	theme	range	426:430	arg1	pH					418:419	pH 7.0	418:423	pH 7.0 (range pH 6.0-8.0)	418:442	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	5	16	theme	whole	572:576	arg1	hydrolysates					583:594	the whole cell hydrolysates	568:594	the whole cell hydrolysates	568:594	Galactose, arabinose and ribose were detected as the sugar compositions in the whole cell hydrolysates.					
31169487	4	17	dep	concentration	459:471	arg1	%					484:484	range 0-5 %	474:484	range 0-5 %	474:484	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	4	17	dep	concentration	459:471	arg1	w/v					487:489	w/v	487:489	w/v	487:489	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	11	18	dep	sp	1542:1543	arg1	name					1498:1501	the name	1494:1501	the name Desertihabitans aurantiacus gen. nov.	1494:1539	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	3	19	theme	Gram-staining-positive	315:336	arg1	cocci					359:363	Gram-staining-positive, aerobic, non-motile cocci	315:363	Gram-staining-positive, aerobic, non-motile cocci	315:363	Cells of the isolate were Gram-staining-positive, aerobic, non-motile cocci.					
31169487	2	20	theme	actinobacterium	129:143	arg1	position					114:121	The taxonomic position	100:121	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T,	100:242	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	10	21	theme	highest	1216:1222	arg1	similarity					1247:1256	the highest 16S rRNA gene sequence similarity	1212:1256	the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T	1212:1310	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	10	22	theme	genus	1182:1186	arg1	Mariniluteicoccus					1188:1204	the genus Mariniluteicoccus	1178:1204	the genus Mariniluteicoccus	1178:1204	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	1	23	theme	novel	51:55	arg1	member					57:62	a novel member	49:62	a novel member of the family Propionibacteriaceae	49:97	nov., a novel member of the family Propionibacteriaceae.					
31169487	1	23	theme	novel	51:55	arg1	nov.					43:46	nov.	43:46	nov.	43:46	nov., a novel member of the family Propionibacteriaceae.					
31169487	7	24	theme	unidentified	779:790	arg1	amino-glycolipid					826:841	one unidentified amino-glycolipid	809:841	one unidentified amino-glycolipid	809:841	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	24	theme	unidentified	779:790	arg1	diphosphatidylglycerol					704:725	diphosphatidylglycerol	704:725	diphosphatidylglycerol	704:725	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	24	theme	unidentified	779:790	arg1	lipids					876:881	the major polar lipids	860:881	the major polar lipids	860:881	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	24	theme	unidentified	779:790	arg1	phosphatidylglycerol					728:747	phosphatidylglycerol	728:747	phosphatidylglycerol	728:747	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	24	theme	unidentified	779:790	arg1	glycolipids					792:802	several unidentified glycolipids	771:802	several unidentified glycolipids	771:802	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	24	theme	unidentified	779:790	arg1	phosphatidylcholine					750:768	phosphatidylcholine	750:768	phosphatidylcholine	750:768	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	12	25	theme	type	1616:1619	arg1	strain					1621:1626	the type strain	1612:1626	the type strain	1612:1626	nov. is proposed, with CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain.					
31169487	10	26	theme	gene	1015:1018	arg1	sequences					1020:1028	16S rRNA gene sequences	1006:1028	16S rRNA gene sequences	1006:1028	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	11	27	theme	aurantiacus	1519:1529	arg1	nov.					1536:1539	Desertihabitans aurantiacus gen. nov.	1503:1539	the name Desertihabitans aurantiacus gen. nov.	1494:1539	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	7	28	theme	major	864:868	arg1	amino-glycolipid					826:841	one unidentified amino-glycolipid	809:841	one unidentified amino-glycolipid	809:841	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	28	theme	major	864:868	arg1	diphosphatidylglycerol					704:725	diphosphatidylglycerol	704:725	diphosphatidylglycerol	704:725	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	28	theme	major	864:868	arg1	lipids					876:881	the major polar lipids	860:881	the major polar lipids	860:881	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	28	theme	major	864:868	arg1	phosphatidylglycerol					728:747	phosphatidylglycerol	728:747	phosphatidylglycerol	728:747	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	28	theme	major	864:868	arg1	glycolipids					792:802	several unidentified glycolipids	771:802	several unidentified glycolipids	771:802	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	28	theme	major	864:868	arg1	phosphatidylcholine					750:768	phosphatidylcholine	750:768	phosphatidylcholine	750:768	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	5	29	from	compositions	552:563	arg1	hydrolysates					583:594	the whole cell hydrolysates	568:594	the whole cell hydrolysates	568:594	Galactose, arabinose and ribose were detected as the sugar compositions in the whole cell hydrolysates.					
31169487	0	30	theme	aurantiacus	16:26	arg1	sp					39:40	sp	39:40	sp	39:40	Desertihabitans aurantiacus gen. nov., sp.					
31169487	0	30	theme	aurantiacus	16:26	arg1	nov.					33:36	Desertihabitans aurantiacus gen. nov.	0:36	Desertihabitans aurantiacus gen. nov.	0:36	Desertihabitans aurantiacus gen. nov., sp.					
31169487	10	31	theme	rRNA	1010:1013	arg1	sequences					1020:1028	16S rRNA gene sequences	1006:1028	16S rRNA gene sequences	1006:1028	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	7	32	theme	polar	870:874	arg1	amino-glycolipid					826:841	one unidentified amino-glycolipid	809:841	one unidentified amino-glycolipid	809:841	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	32	theme	polar	870:874	arg1	diphosphatidylglycerol					704:725	diphosphatidylglycerol	704:725	diphosphatidylglycerol	704:725	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	32	theme	polar	870:874	arg1	lipids					876:881	the major polar lipids	860:881	the major polar lipids	860:881	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	32	theme	polar	870:874	arg1	phosphatidylglycerol					728:747	phosphatidylglycerol	728:747	phosphatidylglycerol	728:747	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	32	theme	polar	870:874	arg1	glycolipids					792:802	several unidentified glycolipids	771:802	several unidentified glycolipids	771:802	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	32	theme	polar	870:874	arg1	phosphatidylcholine					750:768	phosphatidylcholine	750:768	phosphatidylcholine	750:768	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	11	33	from	species	1425:1431	arg1	Propionibacteriaceae					1462:1481	the family Propionibacteriaceae	1451:1481	the family Propionibacteriaceae	1451:1481	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	6	34	theme	peptidoglycan	601:613	arg1	type					615:618	The peptidoglycan type	597:618	The peptidoglycan type	597:618	The peptidoglycan type was A3gamma (ll-Dpm-Gly).					
31169487	6	34	theme	peptidoglycan	601:613	arg1	A3gamma					624:630	A3gamma	624:630	A3gamma (ll-Dpm-Gly)	624:643	The peptidoglycan type was A3gamma (ll-Dpm-Gly).					
31169487	0	35	theme	Desertihabitans	0:14	arg1	sp					39:40	sp	39:40	sp	39:40	Desertihabitans aurantiacus gen. nov., sp.					
31169487	0	35	theme	Desertihabitans	0:14	arg1	nov.					33:36	Desertihabitans aurantiacus gen. nov.	0:36	Desertihabitans aurantiacus gen. nov.	0:36	Desertihabitans aurantiacus gen. nov., sp.					
31169487	10	36	dep	Mariniluteicoccus	1271:1287	arg1	endophyticus					1289:1300	endophyticus	1289:1300	endophyticus	1289:1300	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	5	37	theme	sugar	546:550	arg1	Galactose					493:501	Galactose	493:501	Galactose	493:501	Galactose, arabinose and ribose were detected as the sugar compositions in the whole cell hydrolysates.					
31169487	5	37	theme	sugar	546:550	arg1	ribose					518:523	ribose	518:523	ribose	518:523	Galactose, arabinose and ribose were detected as the sugar compositions in the whole cell hydrolysates.					
31169487	5	37	theme	sugar	546:550	arg1	arabinose					504:512	arabinose	504:512	arabinose	504:512	Galactose, arabinose and ribose were detected as the sugar compositions in the whole cell hydrolysates.					
31169487	5	37	theme	sugar	546:550	arg1	compositions					552:563	the sugar compositions	542:563	the sugar compositions in the whole cell hydrolysates	542:594	Galactose, arabinose and ribose were detected as the sugar compositions in the whole cell hydrolysates.					
31169487	4	38	theme	Good	366:369	arg1	growth					371:376	Good growth	366:376	Good growth	366:376	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	10	39	theme	Phylogenetic	975:986	arg1	analysis					988:995	Phylogenetic analysis	975:995	Phylogenetic analysis based on 16S rRNA gene sequences	975:1028	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	10	40	theme	sequence	1238:1245	arg1	similarity					1247:1256	the highest 16S rRNA gene sequence similarity	1212:1256	the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T	1212:1310	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	4	41	located	observed	382:389	arg1	28 °C					394:398	28 °C	394:398	28 °C (range 20-40 °C)	394:415	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	4	41	located	observed	382:389	arg1	range					401:405	range 20-40 °C	401:414	range 20-40 °C	401:414	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	4	41	located	observed	382:389	arg1	pH					432:433	range pH 6.0-8.0	426:441	range pH 6.0-8.0	426:441	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	4	41	located	observed	382:389	arg1	concentration					459:471	0-1 % NaCl concentration	448:471	0-1 % NaCl concentration (range 0-5 %, w/v)	448:490	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	4	41	located	observed	382:389	arg1	pH					418:419	pH 7.0	418:423	pH 7.0 (range pH 6.0-8.0)	418:442	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	4	41	located	observed	382:389	arg2	growth					371:376	Good growth	366:376	Good growth	366:376	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	10	42	theme	CPCC	1051:1054	arg1	204711T					1056:1062	strain CPCC 204711T	1044:1062	strain CPCC 204711T	1044:1062	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	1	43	theme	family	71:76	arg1	Propionibacteriaceae					78:97	the family Propionibacteriaceae	67:97	the family Propionibacteriaceae	67:97	nov., a novel member of the family Propionibacteriaceae.					
31169487	10	44	theme	gene	1233:1236	arg1	similarity					1247:1256	the highest 16S rRNA gene sequence similarity	1212:1256	the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T	1212:1310	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	0	45	theme	gen.	28:31	arg1	sp					39:40	sp	39:40	sp	39:40	Desertihabitans aurantiacus gen. nov., sp.					
31169487	0	45	theme	gen.	28:31	arg1	nov.					33:36	Desertihabitans aurantiacus gen. nov.	0:36	Desertihabitans aurantiacus gen. nov.	0:36	Desertihabitans aurantiacus gen. nov., sp.					
31169487	7	46	theme	predominant	675:685	arg1	menaquinone					687:697	the predominant menaquinone	671:697	the predominant menaquinone	671:697	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	46	theme	predominant	675:685	arg1	MK-9					646:649	MK-9	646:649	MK-9(H4)	646:653	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	2	47	theme	soil	168:171	arg1	sample					173:178	a desert soil sample	159:178	a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T,	159:242	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	1	48	theme	Propionibacteriaceae	78:97	arg1	member					57:62	a novel member	49:62	a novel member of the family Propionibacteriaceae	49:97	nov., a novel member of the family Propionibacteriaceae.					
31169487	1	48	theme	Propionibacteriaceae	78:97	arg1	nov.					43:46	nov.	43:46	nov.	43:46	nov., a novel member of the family Propionibacteriaceae.					
31169487	10	49	theme	rRNA	1228:1231	arg1	similarity					1247:1256	the highest 16S rRNA gene sequence similarity	1212:1256	the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T	1212:1310	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	12	50	theme	39977T=DSM	1589:1598	arg1	204711T					1574:1580	CPCC 204711T	1569:1580	CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain	1569:1626	nov. is proposed, with CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain.					
31169487	12	50	theme	39977T=DSM	1589:1598	arg1	105431T					1600:1606	=KCTC 39977T=DSM 105431T	1583:1606	=KCTC 39977T=DSM 105431T	1583:1606	nov. is proposed, with CPCC 204711T (=KCTC 39977T=DSM 105431T) as the type strain.					
31169487	11	51	theme	CPCC	1393:1396	arg1	204711T					1398:1404	strain CPCC 204711T	1386:1404	strain CPCC 204711T	1386:1404	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	10	52	theme	phylogenetic	1149:1160	arg1	lineage					1162:1168	a distinct phylogenetic lineage	1138:1168	a distinct phylogenetic lineage next to the genus Mariniluteicoccus	1138:1204	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	2	53	theme	desert	161:166	arg1	sample					173:178	a desert soil sample	159:178	a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T,	159:242	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	10	54	theme	16S	1224:1226	arg1	similarity					1247:1256	the highest 16S rRNA gene sequence similarity	1212:1256	the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T	1212:1310	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	10	55	theme	family	1082:1087	arg1	Propionibacteriaceae					1089:1108	the family Propionibacteriaceae	1078:1108	the family Propionibacteriaceae	1078:1108	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	7	56	theme	several	771:777	arg1	amino-glycolipid					826:841	one unidentified amino-glycolipid	809:841	one unidentified amino-glycolipid	809:841	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	56	theme	several	771:777	arg1	diphosphatidylglycerol					704:725	diphosphatidylglycerol	704:725	diphosphatidylglycerol	704:725	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	56	theme	several	771:777	arg1	lipids					876:881	the major polar lipids	860:881	the major polar lipids	860:881	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	56	theme	several	771:777	arg1	phosphatidylglycerol					728:747	phosphatidylglycerol	728:747	phosphatidylglycerol	728:747	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	56	theme	several	771:777	arg1	glycolipids					792:802	several unidentified glycolipids	771:802	several unidentified glycolipids	771:802	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	56	theme	several	771:777	arg1	phosphatidylcholine					750:768	phosphatidylcholine	750:768	phosphatidylcholine	750:768	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	4	57	theme	 NaCl	453:457	arg1	concentration					459:471	0-1 % NaCl concentration	448:471	0-1 % NaCl concentration (range 0-5 %, w/v)	448:490	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	3	58	dep	Gram-staining-positive	315:336	arg1	non-motile					348:357	non-motile	348:357	non-motile	348:357	Cells of the isolate were Gram-staining-positive, aerobic, non-motile cocci.					
31169487	3	58	dep	Gram-staining-positive	315:336	arg1	aerobic					339:345	aerobic	339:345	aerobic	339:345	Cells of the isolate were Gram-staining-positive, aerobic, non-motile cocci.					
31169487	11	59	theme	new	1438:1440	arg1	genus					1442:1446	a new genus	1436:1446	a new genus in the family Propionibacteriaceae	1436:1481	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	4	60	theme	range	474:478	arg1	%					484:484	range 0-5 %	474:484	range 0-5 %	474:484	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	4	60	theme	range	474:478	arg1	w/v					487:489	w/v	487:489	w/v	487:489	Good growth was observed at 28 °C (range 20-40 °C), pH 7.0 (range pH 6.0-8.0) and 0-1 % NaCl concentration (range 0-5 %, w/v).					
31169487	2	61	theme	Badain	195:200	arg1	Desert					208:213	Badain Jaran Desert	195:213	Badain Jaran Desert	195:213	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	2	62	theme	Jaran	202:206	arg1	Desert					208:213	Badain Jaran Desert	195:213	Badain Jaran Desert	195:213	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	11	63	theme	gen.	1531:1534	arg1	nov.					1536:1539	Desertihabitans aurantiacus gen. nov.	1503:1539	the name Desertihabitans aurantiacus gen. nov.	1494:1539	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	2	64	attach	isolated	145:152	arg2	actinobacterium					129:143	an actinobacterium	126:143	an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T,	126:242	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	2	64	attach	isolated	145:152	arg1	sample					173:178	a desert soil sample	159:178	a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T,	159:242	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	9	65	theme	genomic	936:942	arg1	G+C content					948:958	The genomic DNA G+C content	932:958	The genomic DNA G+C content	932:958	The genomic DNA G+C content was 73.1 mol%.					
31169487	9	65	theme	genomic	936:942	arg1	%					972:972	73.1 mol%	964:972	73.1 mol%	964:972	The genomic DNA G+C content was 73.1 mol%.					
31169487	11	66	theme	Desertihabitans	1503:1517	arg1	nov.					1536:1539	Desertihabitans aurantiacus gen. nov.	1503:1539	the name Desertihabitans aurantiacus gen. nov.	1494:1539	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	9	67	theme	DNA	944:946	arg1	G+C content					948:958	The genomic DNA G+C content	932:958	The genomic DNA G+C content	932:958	The genomic DNA G+C content was 73.1 mol%.					
31169487	9	67	theme	DNA	944:946	arg1	%					972:972	73.1 mol%	964:972	73.1 mol%	964:972	The genomic DNA G+C content was 73.1 mol%.					
31169487	10	68	theme	strain	1044:1049	arg1	204711T					1056:1062	strain CPCC 204711T	1044:1062	strain CPCC 204711T	1044:1062	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	10	69	theme	%	1266:1266	arg1	similarity					1247:1256	the highest 16S rRNA gene sequence similarity	1212:1256	the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T	1212:1310	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	10	70	theme	YIM	1302:1304	arg1	2617T					1306:1310	Mariniluteicoccus endophyticus YIM 2617T	1271:1310	Mariniluteicoccus endophyticus YIM 2617T	1271:1310	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	11	71	theme	novel	1419:1423	arg1	species					1425:1431	a novel species	1417:1431	a novel species	1417:1431	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	7	72	theme	unidentified	813:824	arg1	amino-glycolipid					826:841	one unidentified amino-glycolipid	809:841	one unidentified amino-glycolipid	809:841	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	72	theme	unidentified	813:824	arg1	diphosphatidylglycerol					704:725	diphosphatidylglycerol	704:725	diphosphatidylglycerol	704:725	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	72	theme	unidentified	813:824	arg1	lipids					876:881	the major polar lipids	860:881	the major polar lipids	860:881	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	72	theme	unidentified	813:824	arg1	phosphatidylglycerol					728:747	phosphatidylglycerol	728:747	phosphatidylglycerol	728:747	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	72	theme	unidentified	813:824	arg1	glycolipids					792:802	several unidentified glycolipids	771:802	several unidentified glycolipids	771:802	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	7	72	theme	unidentified	813:824	arg1	phosphatidylcholine					750:768	phosphatidylcholine	750:768	phosphatidylcholine	750:768	MK-9(H4) was detected as the predominant menaquinone, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, several unidentified glycolipids, and one unidentified amino-glycolipid were detected as the major polar lipids.					
31169487	3	73	theme	isolate	302:308	arg1	Cells					289:293	Cells	289:293	Cells of the isolate	289:308	Cells of the isolate were Gram-staining-positive, aerobic, non-motile cocci.					
31169487	2	74	theme	CPCC	230:233	arg1	204711T					235:241	CPCC 204711T	230:241	CPCC 204711T	230:241	The taxonomic position of an actinobacterium isolated from a desert soil sample collected from Badain Jaran Desert, designated as CPCC 204711T, was established using a polyphasic approach.					
31169487	11	75	from	Propionibacteriaceae	1462:1481	arg1	species					1425:1431	a novel species	1417:1431	a novel species	1417:1431	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	10	76	theme	distinct	1140:1147	arg1	lineage					1162:1168	a distinct phylogenetic lineage	1138:1168	a distinct phylogenetic lineage next to the genus Mariniluteicoccus	1138:1204	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	11	77	theme	phylogenetic	1318:1329	arg1	analysis					1331:1338	phylogenetic analysis	1318:1338	phylogenetic analysis	1318:1338	Both phylogenetic analysis and phenotypic characteristics supported that strain CPCC 204711T represents a novel species of a new genus in the family Propionibacteriaceae, for which the name Desertihabitans aurantiacus gen. nov., sp.					
31169487	10	78	theme	16S	1006:1008	arg1	sequences					1020:1028	16S rRNA gene sequences	1006:1028	16S rRNA gene sequences	1006:1028	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204711T affiliated to the family Propionibacteriaceae, in which the strain formed a distinct phylogenetic lineage next to the genus Mariniluteicoccus, with the highest 16S rRNA gene sequence similarity of 96.0 % to Mariniluteicoccus endophyticus YIM 2617T.					
31169487	8	79	theme	predominant	888:898	arg1	 0					928:929	 0	928:929	 0	928:929	The predominant fatty acid was anteiso-C15 : 0.					
31169487	8	79	theme	predominant	888:898	arg1	acid					906:909	The predominant fatty acid	884:909	The predominant fatty acid	884:909	The predominant fatty acid was anteiso-C15 : 0.					
31169487	9	80	theme	73.1 mol	964:971	arg1	G+C content					948:958	The genomic DNA G+C content	932:958	The genomic DNA G+C content	932:958	The genomic DNA G+C content was 73.1 mol%.					
31169487	9	80	theme	73.1 mol	964:971	arg1	%					972:972	73.1 mol%	964:972	73.1 mol%	964:972	The genomic DNA G+C content was 73.1 mol%.					
29331465	12	0	theme	milk	1494:1497	arg1	spectra					1502:1508	the milk IR spectra	1490:1508	the milk IR spectra	1490:1508	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	13	1	used	used	1609:1612	arg2	analyses					1584:1591	milk IR analyses	1576:1591	milk IR analyses	1576:1591	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	10	2	theme	orotic	1252:1257	arg1	acid					1259:1262	orotic acid	1252:1262	orotic acid	1252:1262	On chromosome 1, we identified 2 new genomic regions and hypothesized that they are related to variation in milk phosphorus content and orotic acid, respectively.					
29331465	4	3	theme	individual	545:554	arg1	wavenumbers					559:569	50 individual IR wavenumbers	542:569	50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows	542:607	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	13	4	theme	IR	1731:1732	arg1	spectroscopy					1734:1745	milk IR spectroscopy	1726:1745	milk IR spectroscopy	1726:1745	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	3	5	from	insight	388:394	arg1	relation					403:410	the relation	399:410	the relation between IR wavenumbers and milk components	399:453	This is expected to provide information about the genetic background of milk composition and give insight in the relation between IR wavenumbers and milk components.					
29331465	9	6	theme	collected	1081:1089	arg1	traits					1108:1113	these routinely collected milk composition traits	1065:1113	these routinely collected milk composition traits	1065:1113	However, we also identified some new regions that were not associated with any one of these routinely collected milk composition traits.					
29331465	10	7	theme	genomic	1153:1159	arg1	regions					1161:1167	2 new genomic regions	1147:1167	2 new genomic regions	1147:1167	On chromosome 1, we identified 2 new genomic regions and hypothesized that they are related to variation in milk phosphorus content and orotic acid, respectively.					
29331465	13	8	theme	milk	1576:1579	arg1	analyses					1584:1591	milk IR analyses	1576:1591	milk IR analyses	1576:1591	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	2	9	theme	genomic	226:232	arg1	regions					234:240	genomic regions	226:240	genomic regions associated with individual milk IR wavenumbers	226:287	The objective of this study was to identify genomic regions associated with individual milk IR wavenumbers.					
29331465	8	10	theme	lactose	959:965	arg1	percentage					967:976	lactose percentage	959:976	lactose percentage	959:976	Most of these regions also showed significant associations with fat, protein, or lactose percentage.					
29331465	9	11	theme	milk	1091:1094	arg1	traits					1108:1113	these routinely collected milk composition traits	1065:1113	these routinely collected milk composition traits	1065:1113	However, we also identified some new regions that were not associated with any one of these routinely collected milk composition traits.					
29331465	2	12	theme	milk	269:272	arg1	wavenumbers					277:287	individual milk IR wavenumbers	258:287	individual milk IR wavenumbers	258:287	The objective of this study was to identify genomic regions associated with individual milk IR wavenumbers.					
29331465	10	13	theme	new	1149:1151	arg1	regions					1161:1167	2 new genomic regions	1147:1167	2 new genomic regions	1147:1167	On chromosome 1, we identified 2 new genomic regions and hypothesized that they are related to variation in milk phosphorus content and orotic acid, respectively.					
29331465	4	14	theme	wavenumbers	559:569	arg1	wavenumbers					559:569	50 individual IR wavenumbers	542:569	50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows	542:607	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	4	14	theme	wavenumbers	559:569	arg1	set					535:537	a selected set	524:537	a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows	524:607	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	3	15	theme	genetic	340:346	arg1	background					348:357	the genetic background	336:357	the genetic background of milk composition	336:377	This is expected to provide information about the genetic background of milk composition and give insight in the relation between IR wavenumbers and milk components.					
29331465	8	16	theme	significant	912:922	arg1	associations					924:935	significant associations	912:935	significant associations with fat, protein, or lactose percentage	912:976	Most of these regions also showed significant associations with fat, protein, or lactose percentage.					
29331465	2	17	theme	individual	258:267	arg1	wavenumbers					277:287	individual milk IR wavenumbers	258:287	individual milk IR wavenumbers	258:287	The objective of this study was to identify genomic regions associated with individual milk IR wavenumbers.					
29331465	4	18	theme	IR	556:557	arg1	wavenumbers					559:569	50 individual IR wavenumbers	542:569	50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows	542:607	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	13	19	theme	low	1659:1661	arg1	concentrations					1663:1676	low concentrations	1659:1676	low concentrations in milk	1659:1684	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	9	20	theme	traits	1108:1113	arg1	traits					1108:1113	these routinely collected milk composition traits	1065:1113	these routinely collected milk composition traits	1065:1113	However, we also identified some new regions that were not associated with any one of these routinely collected milk composition traits.					
29331465	9	20	theme	traits	1108:1113	arg1	one					1058:1060	one	1058:1060	one	1058:1060	However, we also identified some new regions that were not associated with any one of these routinely collected milk composition traits.					
29331465	13	21	theme	milk	1726:1729	arg1	spectroscopy					1734:1745	milk IR spectroscopy	1726:1745	milk IR spectroscopy	1726:1745	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	8	22	with	associations	924:935	arg1	protein					947:953	protein	947:953	protein	947:953	Most of these regions also showed significant associations with fat, protein, or lactose percentage.					
29331465	8	22	with	associations	924:935	arg1	fat					942:944	fat	942:944	fat	942:944	Most of these regions also showed significant associations with fat, protein, or lactose percentage.					
29331465	8	22	with	associations	924:935	arg1	percentage					967:976	lactose percentage	959:976	lactose percentage	959:976	Most of these regions also showed significant associations with fat, protein, or lactose percentage.					
29331465	13	23	theme	novel	1704:1708	arg1	applications					1710:1721	novel applications	1704:1721	novel applications of milk IR spectroscopy for dairy cattle breeding and herd management	1704:1791	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	0	24	theme	Genome-wide	0:10	arg1	association					12:22	Genome-wide association	0:22	Genome-wide association	0:22	Genome-wide association study for milk infrared wavenumbers.					
29331465	11	25	theme	new	1313:1315	arg1	region					1325:1330	a new genomic region	1311:1330	a new genomic region that seems to be related to citric acid	1311:1370	On chromosome 20, we identified a new genomic region that seems to be related to citric acid.					
29331465	11	25	theme	new	1313:1315	arg1	related					1349:1355	related	1349:1355	related	1349:1355	On chromosome 20, we identified a new genomic region that seems to be related to citric acid.					
29331465	3	26	theme	IR	420:421	arg1	wavenumbers					423:433	IR wavenumbers	420:433	IR wavenumbers	420:433	This is expected to provide information about the genetic background of milk composition and give insight in the relation between IR wavenumbers and milk components.					
29331465	3	27	theme	milk	362:365	arg1	composition					367:377	milk composition	362:377	milk composition	362:377	This is expected to provide information about the genetic background of milk composition and give insight in the relation between IR wavenumbers and milk components.					
29331465	1	28	theme	infrared	91:98	arg1	spectra					105:111	the infrared (IR) spectra	87:111	the infrared (IR) spectra of bovine milk	87:126	Individual wavenumbers of the infrared (IR) spectra of bovine milk have been shown to be moderately to highly heritable.					
29331465	11	29	theme	genomic	1317:1323	arg1	region					1325:1330	a new genomic region	1311:1330	a new genomic region that seems to be related to citric acid	1311:1370	On chromosome 20, we identified a new genomic region that seems to be related to citric acid.					
29331465	11	29	theme	genomic	1317:1323	arg1	related					1349:1355	related	1349:1355	related	1349:1355	On chromosome 20, we identified a new genomic region that seems to be related to citric acid.					
29331465	3	30	theme	composition	367:377	arg1	background					348:357	the genetic background	336:357	the genetic background of milk composition	336:377	This is expected to provide information about the genetic background of milk composition and give insight in the relation between IR wavenumbers and milk components.					
29331465	4	31	theme	selected	526:533	arg1	wavenumbers					559:569	50 individual IR wavenumbers	542:569	50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows	542:607	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	4	31	theme	selected	526:533	arg1	set					535:537	a selected set	524:537	a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows	524:607	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	5	32	theme	Significant	610:620	arg1	associations					622:633	Significant associations	610:633	Significant associations	610:633	Significant associations were detected for 28 of the 50 wavenumbers.					
29331465	7	33	theme	IR	807:808	arg1	wavenumbers					810:820	milk IR wavenumbers	802:820	milk IR wavenumbers	802:820	Major genomic regions associated with milk IR wavenumbers were identified on chromosomes 1, 5, 6, 14, 19, and 20.					
29331465	13	34	from	concentrations	1663:1676	arg1	milk					1681:1684	milk	1681:1684	milk	1681:1684	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	13	35	contain	have	1654:1657	arg2	concentrations					1663:1676	low concentrations	1659:1676	low concentrations in milk	1659:1684	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	13	35	contain	have	1654:1657	arg1	components					1636:1645	these milk components	1625:1645	these milk components	1625:1645	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	5	36	theme	wavenumbers	666:676	arg1	28					653:654	28	653:654	28	653:654	Significant associations were detected for 28 of the 50 wavenumbers.					
29331465	5	36	theme	wavenumbers	666:676	arg1	wavenumbers					666:676	the 50 wavenumbers	659:676	the 50 wavenumbers	659:676	Significant associations were detected for 28 of the 50 wavenumbers.					
29331465	1	37	theme	IR	101:102	arg1	spectra					105:111	the infrared (IR) spectra	87:111	the infrared (IR) spectra of bovine milk	87:126	Individual wavenumbers of the infrared (IR) spectra of bovine milk have been shown to be moderately to highly heritable.					
29331465	12	38	theme	IR	1499:1500	arg1	spectra					1502:1508	the milk IR spectra	1490:1508	the milk IR spectra	1490:1508	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	3	39	theme	milk	439:442	arg1	components					444:453	milk components	439:453	milk components	439:453	This is expected to provide information about the genetic background of milk composition and give insight in the relation between IR wavenumbers and milk components.					
29331465	13	40	theme	IR	1581:1582	arg1	analyses					1584:1591	milk IR analyses	1576:1591	milk IR analyses	1576:1591	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	7	41	theme	Major	764:768	arg1	regions					778:784	Major genomic regions	764:784	Major genomic regions associated with milk IR wavenumbers	764:820	Major genomic regions associated with milk IR wavenumbers were identified on chromosomes 1, 5, 6, 14, 19, and 20.					
29331465	12	42	from	information	1525:1535	arg1	components					1551:1560	these milk components	1540:1560	these milk components	1540:1560	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	2	43	theme	IR	274:275	arg1	wavenumbers					277:287	individual milk IR wavenumbers	258:287	individual milk IR wavenumbers	258:287	The objective of this study was to identify genomic regions associated with individual milk IR wavenumbers.					
29331465	12	44	theme	direct	1518:1523	arg1	information					1525:1535	direct information	1518:1535	direct information on these milk components	1518:1560	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	4	45	theme	Holstein	595:602	arg1	cows					604:607	1,748 Dutch Holstein cows	583:607	1,748 Dutch Holstein cows	583:607	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	0	46	theme	infrared	39:46	arg1	wavenumbers					48:58	milk infrared wavenumbers	34:58	milk infrared wavenumbers	34:58	Genome-wide association study for milk infrared wavenumbers.					
29331465	7	47	theme	genomic	770:776	arg1	regions					778:784	Major genomic regions	764:784	Major genomic regions associated with milk IR wavenumbers	764:820	Major genomic regions associated with milk IR wavenumbers were identified on chromosomes 1, 5, 6, 14, 19, and 20.					
29331465	13	48	theme	dairy	1751:1755	arg1	breeding					1764:1771	dairy cattle breeding and herd management	1751:1791	breeding	1764:1771	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	7	49	theme	milk	802:805	arg1	wavenumbers					810:820	milk IR wavenumbers	802:820	milk IR wavenumbers	802:820	Major genomic regions associated with milk IR wavenumbers were identified on chromosomes 1, 5, 6, 14, 19, and 20.					
29331465	0	50	theme	milk	34:37	arg1	wavenumbers					48:58	milk infrared wavenumbers	34:58	milk infrared wavenumbers	34:58	Genome-wide association study for milk infrared wavenumbers.					
29331465	6	51	theme	bovine	728:733	arg1	chromosomes					735:745	16 bovine chromosomes	725:745	16 bovine chromosomes	725:745	In total, 24 genomic regions distributed over 16 bovine chromosomes were identified.					
29331465	2	52	theme	study	204:208	arg1	objective					186:194	The objective	182:194	The objective of this study	182:208	The objective of this study was to identify genomic regions associated with individual milk IR wavenumbers.					
29331465	4	53	theme	association	488:498	arg1	study					500:504	a genome-wide association study	474:504	a genome-wide association study	474:504	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	9	54	theme	composition	1096:1106	arg1	traits					1108:1113	these routinely collected milk composition traits	1065:1113	these routinely collected milk composition traits	1065:1113	However, we also identified some new regions that were not associated with any one of these routinely collected milk composition traits.					
29331465	12	55	theme	genomic	1391:1397	arg1	regions					1399:1405	genomic regions	1391:1405	genomic regions associated with milk phosphorus content, orotic acid, and citric acid	1391:1475	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	1	56	theme	spectra	105:111	arg1	wavenumbers					72:82	Individual wavenumbers	61:82	Individual wavenumbers of the infrared (IR) spectra of bovine milk	61:126	Individual wavenumbers of the infrared (IR) spectra of bovine milk have been shown to be moderately to highly heritable.					
29331465	4	57	theme	genome-wide	476:486	arg1	association					488:498	a genome-wide association	474:498	a genome-wide association study	474:504	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	1	58	dep	moderately	150:159	arg1	to					161:162	to	161:162	to	161:162	Individual wavenumbers of the infrared (IR) spectra of bovine milk have been shown to be moderately to highly heritable.					
29331465	10	59	theme	phosphorus	1229:1238	arg1	content					1240:1246	milk phosphorus content	1224:1246	milk phosphorus content	1224:1246	On chromosome 1, we identified 2 new genomic regions and hypothesized that they are related to variation in milk phosphorus content and orotic acid, respectively.					
29331465	12	60	theme	regions	1399:1405	arg1	Identification					1373:1386	Identification	1373:1386	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid	1373:1475	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	12	61	theme	citric	1465:1470	arg1	acid					1472:1475	citric acid	1465:1475	citric acid	1465:1475	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	13	62	theme	milk	1631:1634	arg1	components					1636:1645	these milk components	1625:1645	these milk components	1625:1645	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	9	63	theme	new	1012:1014	arg1	regions					1016:1022	some new regions	1007:1022	some new regions that were not associated with any one of these routinely collected milk composition traits	1007:1113	However, we also identified some new regions that were not associated with any one of these routinely collected milk composition traits.					
29331465	1	64	theme	bovine	116:121	arg1	milk					123:126	bovine milk	116:126	bovine milk	116:126	Individual wavenumbers of the infrared (IR) spectra of bovine milk have been shown to be moderately to highly heritable.					
29331465	10	65	theme	milk	1224:1227	arg1	content					1240:1246	milk phosphorus content	1224:1246	milk phosphorus content	1224:1246	On chromosome 1, we identified 2 new genomic regions and hypothesized that they are related to variation in milk phosphorus content and orotic acid, respectively.					
29331465	1	66	theme	Individual	61:70	arg1	wavenumbers					72:82	Individual wavenumbers	61:82	Individual wavenumbers of the infrared (IR) spectra of bovine milk	61:126	Individual wavenumbers of the infrared (IR) spectra of bovine milk have been shown to be moderately to highly heritable.					
29331465	1	67	theme	milk	123:126	arg1	spectra					105:111	the infrared (IR) spectra	87:111	the infrared (IR) spectra of bovine milk	87:126	Individual wavenumbers of the infrared (IR) spectra of bovine milk have been shown to be moderately to highly heritable.					
29331465	13	68	theme	herd	1777:1780	arg1	management					1782:1791	dairy cattle breeding and herd management	1751:1791	management	1782:1791	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	12	69	theme	milk	1546:1549	arg1	components					1551:1560	these milk components	1540:1560	these milk components	1540:1560	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	13	70	theme	cattle	1757:1762	arg1	breeding					1764:1771	dairy cattle breeding and herd management	1751:1791	breeding	1764:1771	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	12	71	theme	milk	1423:1426	arg1	content					1439:1445	milk phosphorus content	1423:1445	milk phosphorus content	1423:1445	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	6	72	theme	genomic	692:698	arg1	regions					700:706	24 genomic regions	689:706	24 genomic regions distributed over 16 bovine chromosomes	689:745	In total, 24 genomic regions distributed over 16 bovine chromosomes were identified.					
29331465	11	73	theme	citric	1360:1365	arg1	acid					1367:1370	citric acid	1360:1370	citric acid	1360:1370	On chromosome 20, we identified a new genomic region that seems to be related to citric acid.					
29331465	4	74	theme	Dutch	589:593	arg1	cows					604:607	1,748 Dutch Holstein cows	583:607	1,748 Dutch Holstein cows	583:607	For this purpose, a genome-wide association study was performed for a selected set of 50 individual IR wavenumbers measured on 1,748 Dutch Holstein cows.					
29331465	12	75	theme	phosphorus	1428:1437	arg1	content					1439:1445	milk phosphorus content	1423:1445	milk phosphorus content	1423:1445	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	12	76	contain	contain	1510:1516	arg1	spectra					1502:1508	the milk IR spectra	1490:1508	the milk IR spectra	1490:1508	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	12	76	contain	contain	1510:1516	arg2	information					1525:1535	direct information	1518:1535	direct information on these milk components	1518:1560	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29331465	10	77	from	variation	1211:1219	arg1	content					1240:1246	milk phosphorus content	1224:1246	milk phosphorus content	1224:1246	On chromosome 1, we identified 2 new genomic regions and hypothesized that they are related to variation in milk phosphorus content and orotic acid, respectively.					
29331465	10	77	from	variation	1211:1219	arg1	acid					1259:1262	orotic acid	1252:1262	orotic acid	1252:1262	On chromosome 1, we identified 2 new genomic regions and hypothesized that they are related to variation in milk phosphorus content and orotic acid, respectively.					
29331465	13	78	theme	spectroscopy	1734:1745	arg1	applications					1710:1721	novel applications	1704:1721	novel applications of milk IR spectroscopy for dairy cattle breeding and herd management	1704:1791	Consequently milk IR analyses probably can be used to predict these milk components, which have low concentrations in milk; this can lead to novel applications of milk IR spectroscopy for dairy cattle breeding and herd management.					
29331465	12	79	theme	orotic	1448:1453	arg1	acid					1455:1458	orotic acid	1448:1458	orotic acid	1448:1458	Identification of genomic regions associated with milk phosphorus content, orotic acid, and citric acid suggest that the milk IR spectra contain direct information on these milk components.					
29759647	2	0	theme	complexation	559:570	arg1	application					603:613	the application	599:613	the application of an additional water plug between sample and buffer plug	599:672	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	0	theme	complexation	559:570	arg1	electrolyte					486:496	the background electrolyte	471:496	the background electrolyte	471:496	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	0	theme	complexation	559:570	arg1	use					503:505	the use	499:505	the use of a selector free buffer plug	499:536	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	0	theme	complexation	559:570	arg1	contribution					543:554	the contribution	539:554	the contribution of complexation within the buffer plug	539:593	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	1	1	theme	chiral	311:316	arg1	selectors					318:326	negatively charged chiral selectors	292:326	negatively charged chiral selectors	292:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	0	2	theme	capillary	96:104	arg1	electrophoresis					106:120	enantioselective capillary electrophoresis	79:120	enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors	79:161	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.					
29759647	4	3	theme	phosphate	933:941	arg1	buffers					943:949	chiral selector and phosphate buffers	913:949	buffers	943:949	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	0	4	with	electrophoresis	106:120	arg1	cyclodextrins					136:148	sulfated cyclodextrins	127:148	sulfated cyclodextrins as selectors	127:161	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.					
29759647	1	5	theme	cations	211:217	arg1	stacking					199:206	Head-column field-amplified sample stacking	164:206	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors	164:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	5	6	theme	buffer	1037:1042	arg1	plug					1044:1047	the buffer plug	1033:1047	the buffer plug	1033:1047	Results confirm that the buffer plug is providing a spacer between cationic analytes and the negatively charged selector during electrokinetic injection.					
29759647	0	7	theme	sulfated	127:134	arg1	cyclodextrins					136:148	sulfated cyclodextrins	127:148	sulfated cyclodextrins as selectors	127:161	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.					
29759647	2	8	theme	buffer	662:667	arg1	plug					669:672	buffer plug	662:672	buffer plug	662:672	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	7	9	theme	best	1429:1432	arg1	conditions					1434:1443	best conditions	1429:1443	best conditions	1429:1443	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	6	10	with	interference	1273:1284	arg1	selector					1295:1302	the selector	1291:1302	the selector	1291:1302	Simulation predicts the required length and composition of the plug for a given injection time to avoid an interference with the selector.					
29759647	4	11	theme	buffer	999:1004	arg1	plug					1006:1009	the buffer plug	995:1009	the buffer plug	995:1009	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	7	12	theme	sulfated	1510:1517	arg1	cyclodextrins					1519:1531	sulfated cyclodextrins	1510:1531	sulfated cyclodextrins as selectors	1510:1544	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	6	13	theme	injection	1246:1254	arg1	time					1256:1259	a given injection time	1238:1259	a given injection time to avoid an interference with the selector	1238:1302	Simulation predicts the required length and composition of the plug for a given injection time to avoid an interference with the selector.					
29759647	6	14	theme	required	1190:1197	arg1	length					1199:1204	required length	1190:1204	required length	1190:1204	Simulation predicts the required length and composition of the plug for a given injection time to avoid an interference with the selector.					
29759647	7	15	theme	water	1313:1317	arg1	plug					1319:1322	A short water plug	1305:1322	A short water plug added between the low conductivity sample and a high conductivity buffer plug	1305:1400	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	4	16	theme	chiral	913:918	arg1	selector					920:927	chiral selector and phosphate buffers	913:949	selector	920:927	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	4	16	theme	chiral	913:918	arg1	pH					952:953	pH 6.3	952:957	pH 6.3	952:957	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	6	17	theme	given	1240:1244	arg1	time					1256:1259	a given injection time	1238:1259	a given injection time to avoid an interference with the selector	1238:1302	Simulation predicts the required length and composition of the plug for a given injection time to avoid an interference with the selector.					
29759647	7	18	theme	high	1372:1375	arg1	buffer					1390:1395	a high conductivity buffer	1370:1395	a high conductivity buffer	1370:1395	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	2	19	theme	buffer	583:588	arg1	plug					590:593	the buffer plug	579:593	the buffer plug	579:593	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	20	theme	free	521:524	arg1	plug					533:536	a selector free buffer plug	510:536	a selector free buffer plug	510:536	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	21	theme	background	475:484	arg1	electrolyte					486:496	the background electrolyte	471:496	the background electrolyte	471:496	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	22	theme	buffer	526:531	arg1	plug					533:536	a selector free buffer plug	510:536	a selector free buffer plug	510:536	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	4	23	dep	selected	835:842	arg1	substituted					874:884	substituted	874:884	substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug	874:1009	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	1	24	theme	low	226:228	arg1	sample					243:248	a low conductivity sample	224:248	a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors	224:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	0	25	theme	Fundamental	0:10	arg1	aspects					12:18	Fundamental aspects	0:18	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.	0:162	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.					
29759647	6	26	theme	plug	1229:1232	arg1	length					1199:1204	required length	1190:1204	required length	1190:1204	Simulation predicts the required length and composition of the plug for a given injection time to avoid an interference with the selector.					
29759647	6	26	theme	plug	1229:1232	arg1	composition					1210:1220	composition	1210:1220	composition	1210:1220	Simulation predicts the required length and composition of the plug for a given injection time to avoid an interference with the selector.					
29759647	1	27	theme	conductivity	230:241	arg1	sample					243:248	a low conductivity sample	224:248	a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors	224:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	0	28	theme	field-amplified	23:37	arg1	injection					54:62	field-amplified electrokinetic injection	23:62	field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors	23:161	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.					
29759647	2	29	theme	additional	621:630	arg1	plug					638:641	an additional water plug	618:641	an additional water plug between sample and buffer plug	618:672	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	4	30	theme	Racemic	813:819	arg1	compound					855:862	a model compound	847:862	a model compound	847:862	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	4	30	theme	Racemic	813:819	arg1	methadone					821:829	Racemic methadone	813:829	Racemic methadone	813:829	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	7	31	theme	enantioselective	1476:1491	arg1	assays					1498:1503	enantioselective drug assays	1476:1503	enantioselective drug assays with sulfated cyclodextrins as selectors	1476:1544	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	3	32	theme	effective	793:801	arg1	mobility					803:810	effective mobility	793:810	effective mobility	793:810	Attention was paid for changes of ionic strength which is known to have a significant impact on complexation and thus effective mobility.					
29759647	4	33	theme	background	968:977	arg1	electrolyte					979:989	the background electrolyte	964:989	the background electrolyte	964:989	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	3	34	theme	ionic	709:713	arg1	strength					715:722	ionic strength	709:722	ionic strength which is known to have a significant impact on complexation and thus effective mobility	709:810	Attention was paid for changes of ionic strength which is known to have a significant impact on complexation and thus effective mobility.					
29759647	7	35	theme	short	1307:1311	arg1	plug					1319:1322	A short water plug	1305:1322	A short water plug added between the low conductivity sample and a high conductivity buffer plug	1305:1400	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	2	36	theme	analytes	457:464	arg1	injection					440:448	the direct electrokinetic injection	414:448	the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug	414:672	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	0	37	theme	injection	54:62	arg1	aspects					12:18	Fundamental aspects	0:18	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.	0:162	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.					
29759647	1	38	theme	computer	364:371	arg1	simulation					373:382	computer simulation	364:382	computer simulation	364:382	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	3	39	theme	strength	715:722	arg1	changes					698:704	changes	698:704	changes of ionic strength which is known to have a significant impact on complexation and thus effective mobility	698:810	Attention was paid for changes of ionic strength which is known to have a significant impact on complexation and thus effective mobility.					
29759647	7	40	theme	conductivity	1377:1388	arg1	buffer					1390:1395	a high conductivity buffer	1370:1395	a high conductivity buffer	1370:1395	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	7	41	theme	conductivity	1346:1357	arg1	sample					1359:1364	the low conductivity sample	1338:1364	the low conductivity sample	1338:1364	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	0	42	theme	electrokinetic	39:52	arg1	injection					54:62	field-amplified electrokinetic injection	23:62	field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors	23:161	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.					
29759647	7	43	with	assays	1498:1503	arg1	cyclodextrins					1519:1531	sulfated cyclodextrins	1510:1531	sulfated cyclodextrins as selectors	1510:1544	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	5	44	theme	electrokinetic	1140:1153	arg1	injection					1155:1163	electrokinetic injection	1140:1163	electrokinetic injection	1140:1163	Results confirm that the buffer plug is providing a spacer between cationic analytes and the negatively charged selector during electrokinetic injection.					
29759647	2	45	theme	selector	512:519	arg1	plug					533:536	a selector free buffer plug	510:536	a selector free buffer plug	510:536	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	1	46	theme	enantiomeric	262:273	arg1	separation					275:284	enantiomeric separation	262:284	enantiomeric separation using negatively charged chiral selectors	262:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	0	47	theme	cations	67:73	arg1	injection					54:62	field-amplified electrokinetic injection	23:62	field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors	23:161	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.					
29759647	5	48	theme	cationic	1079:1086	arg1	analytes					1088:1095	cationic analytes	1079:1095	cationic analytes	1079:1095	Results confirm that the buffer plug is providing a spacer between cationic analytes and the negatively charged selector during electrokinetic injection.					
29759647	2	49	theme	electrokinetic	425:438	arg1	injection					440:448	the direct electrokinetic injection	414:448	the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug	414:672	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	50	theme	plug	533:536	arg1	application					603:613	the application	599:613	the application of an additional water plug between sample and buffer plug	599:672	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	50	theme	plug	533:536	arg1	electrolyte					486:496	the background electrolyte	471:496	the background electrolyte	471:496	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	50	theme	plug	533:536	arg1	use					503:505	the use	499:505	the use of a selector free buffer plug	499:536	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	50	theme	plug	533:536	arg1	contribution					543:554	the contribution	539:554	the contribution of complexation within the buffer plug	539:593	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	4	51	theme	sulfated	886:893	arg1	β-cyclodextrin					895:908	sulfated β-cyclodextrin	886:908	sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3)	886:958	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	1	52	from	sample	243:248	arg1	stacking					199:206	Head-column field-amplified sample stacking	164:206	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors	164:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	2	53	theme	direct	418:423	arg1	injection					440:448	the direct electrokinetic injection	414:448	the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug	414:672	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	54	theme	plug	638:641	arg1	application					603:613	the application	599:613	the application of an additional water plug between sample and buffer plug	599:672	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	54	theme	plug	638:641	arg1	electrolyte					486:496	the background electrolyte	471:496	the background electrolyte	471:496	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	54	theme	plug	638:641	arg1	use					503:505	the use	499:505	the use of a selector free buffer plug	499:536	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	2	54	theme	plug	638:641	arg1	contribution					543:554	the contribution	539:554	the contribution of complexation within the buffer plug	539:593	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	6	55	dep	length	1199:1204	arg1	the					1186:1188	the	1186:1188	the	1186:1188	Simulation predicts the required length and composition of the plug for a given injection time to avoid an interference with the selector.					
29759647	4	56	theme	model	849:853	arg1	compound					855:862	a model compound	847:862	a model compound	847:862	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	4	56	theme	model	849:853	arg1	methadone					821:829	Racemic methadone	813:829	Racemic methadone	813:829	Racemic methadone was selected as a model compound, randomly substituted sulfated β-cyclodextrin as chiral selector and phosphate buffers (pH 6.3) for the background electrolyte and the buffer plug.					
29759647	7	57	theme	drug	1493:1496	arg1	assays					1498:1503	enantioselective drug assays	1476:1503	enantioselective drug assays with sulfated cyclodextrins as selectors	1476:1544	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	1	58	theme	Head-column	164:174	arg1	stacking					199:206	Head-column field-amplified sample stacking	164:206	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors	164:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	7	59	theme	high	1456:1459	arg1	sensitivity					1461:1471	high sensitivity	1456:1471	high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors	1456:1544	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	2	60	theme	water	632:636	arg1	plug					638:641	an additional water plug	618:641	an additional water plug between sample and buffer plug	618:672	Aspects investigated include the direct electrokinetic injection of the analytes into the background electrolyte, the use of a selector free buffer plug, the contribution of complexation within the buffer plug and the application of an additional water plug between sample and buffer plug.					
29759647	7	61	dep	sample	1359:1364	arg1	plug					1397:1400	plug	1397:1400	plug	1397:1400	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	1	62	theme	field-amplified	176:190	arg1	stacking					199:206	Head-column field-amplified sample stacking	164:206	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors	164:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	0	63	theme	enantioselective	79:94	arg1	electrophoresis					106:120	enantioselective capillary electrophoresis	79:120	enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors	79:161	Fundamental aspects of field-amplified electrokinetic injection of cations for enantioselective capillary electrophoresis with sulfated cyclodextrins as selectors.					
29759647	5	64	theme	charged	1116:1122	arg1	selector					1124:1131	the negatively charged selector	1101:1131	the negatively charged selector during electrokinetic injection	1101:1163	Results confirm that the buffer plug is providing a spacer between cationic analytes and the negatively charged selector during electrokinetic injection.					
29759647	1	65	theme	sample	192:197	arg1	stacking					199:206	Head-column field-amplified sample stacking	164:206	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors	164:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
29759647	7	66	from	sensitivity	1461:1471	arg1	assays					1498:1503	enantioselective drug assays	1476:1503	enantioselective drug assays with sulfated cyclodextrins as selectors	1476:1544	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	3	67	theme	significant	749:759	arg1	impact					761:766	a significant impact	747:766	a significant impact	747:766	Attention was paid for changes of ionic strength which is known to have a significant impact on complexation and thus effective mobility.					
29759647	7	68	theme	low	1342:1344	arg1	sample					1359:1364	the low conductivity sample	1338:1364	the low conductivity sample	1338:1364	A short water plug added between the low conductivity sample and a high conductivity buffer plug is demonstrated to provide best conditions to achieve high sensitivity in enantioselective drug assays with sulfated cyclodextrins as selectors.					
29759647	3	69	contain	have	742:745	arg1	strength					715:722	ionic strength	709:722	ionic strength which is known to have a significant impact on complexation and thus effective mobility	709:810	Attention was paid for changes of ionic strength which is known to have a significant impact on complexation and thus effective mobility.					
29759647	3	69	contain	have	742:745	arg2	impact					761:766	a significant impact	747:766	a significant impact	747:766	Attention was paid for changes of ionic strength which is known to have a significant impact on complexation and thus effective mobility.					
29759647	1	70	theme	charged	303:309	arg1	selectors					318:326	negatively charged chiral selectors	292:326	negatively charged chiral selectors	292:326	Head-column field-amplified sample stacking of cations from a low conductivity sample followed by enantiomeric separation using negatively charged chiral selectors was studied experimentally and with computer simulation.					
30242260	0	0	theme	frequent	63:70	arg1	consumption					80:90	frequent sucrose consumption	63:90	frequent sucrose consumption	63:90	In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.					
30242260	9	1	theme	dental	1188:1193	arg1	surface					1202:1208	dental enamel surface	1188:1208	dental enamel surface	1188:1208	Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface.					
30242260	8	2	theme	streptococci	1003:1014	arg1	abundance					990:998	abundance	990:998	abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis	990:1108	It was marked by a significant increase in abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis.					
30242260	10	3	from	synergy	1444:1450	arg1	development					1489:1499	the development	1485:1499	the development of caries	1485:1509	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	8	4	from	increase	978:985	arg1	abundance					990:998	abundance	990:998	abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis	990:1108	It was marked by a significant increase in abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis.					
30242260	0	5	from	shift	8:12	arg1	biofilm					40:46	oral biofilm	35:46	oral biofilm in response to frequent sucrose consumption	35:90	In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.					
30242260	6	6	theme	enamel	769:774	arg1	surface					776:782	the enamel surface	765:782	the enamel surface	765:782	In addition, roughness of the enamel surface was measured with laser scanning microscopy.					
30242260	1	7	theme	dental	143:148	arg1	biofilms					150:157	dental biofilms	143:157	dental biofilms	143:157	Caries is associated with shifts of microbiota in dental biofilms and primarily driven by frequent sucrose consumption.					
30242260	2	8	from	influence	312:320	arg1	biofilm					366:372	the oral biofilm	357:372	the oral biofilm	357:372	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	5	9	theme	Illumina	688:695	arg1	sequencing					712:721	Illumina MiSeq amplicon sequencing	688:721	Illumina MiSeq amplicon sequencing (v1-v2 region)	688:736	The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region).					
30242260	5	9	theme	Illumina	688:695	arg1	region					730:735	v1-v2 region	724:735	v1-v2 region	724:735	The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region).					
30242260	4	10	theme	three-month	500:510	arg1	change					520:525	a three-month dietary change	498:525	a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet	498:592	After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase.					
30242260	9	11	theme	enamel	1195:1200	arg1	surface					1202:1208	dental enamel surface	1188:1208	dental enamel surface	1188:1208	Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface.					
30242260	7	12	theme	sucrose	833:839	arg1	phase					841:845	The sucrose phase	829:845	The sucrose phase	829:845	The sucrose phase resulted in significant differences in beta-diversity and significantly decreased species richness.					
30242260	0	13	theme	sucrose	72:78	arg1	consumption					80:90	frequent sucrose consumption	63:90	frequent sucrose consumption	63:90	In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.					
30242260	0	14	from	biofilm	40:46	arg1	response					51:58	response	51:58	response to frequent sucrose consumption	51:90	In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.					
30242260	10	15	theme	species	1474:1480	arg1	synergy					1444:1450	the synergy	1440:1450	the synergy of multiple bacterial species in the development of caries	1440:1509	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	7	16	theme	species	929:935	arg1	richness					937:944	species richness	929:944	species richness	929:944	The sucrose phase resulted in significant differences in beta-diversity and significantly decreased species richness.					
30242260	2	17	from	Data	213:216	arg1	shifts					264:269	environmentally induced in vivo microbiota shifts	221:269	environmentally induced in vivo microbiota shifts	221:269	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	3	18	theme	biofilm	475:481	arg1	samples					483:489	oral biofilm samples	470:489	oral biofilm samples	470:489	Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples.					
30242260	5	19	theme	amplicon	703:710	arg1	sequencing					712:721	Illumina MiSeq amplicon sequencing	688:721	Illumina MiSeq amplicon sequencing (v1-v2 region)	688:736	The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region).					
30242260	5	19	theme	amplicon	703:710	arg1	region					730:735	v1-v2 region	724:735	v1-v2 region	724:735	The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region).					
30242260	8	20	theme	significant	966:976	arg1	increase					978:985	a significant increase	964:985	a significant increase in abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis	964:1108	It was marked by a significant increase in abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis.					
30242260	10	21	theme	compositional	1273:1285	arg1	changes					1287:1293	compositional changes	1273:1293	compositional changes in the microbiota	1273:1311	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	2	22	dep	in	245:246	arg1	vivo					248:251	vivo	248:251	vivo	248:251	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	10	23	theme	bacterial	1464:1472	arg1	species					1474:1480	multiple bacterial species	1455:1480	multiple bacterial species	1455:1480	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	4	24	theme	regular	581:587	arg1	diet					589:592	the regular diet	577:592	the regular diet	577:592	After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase.					
30242260	2	25	dep	induced	237:243	arg1	in					245:246	in	245:246	in	245:246	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	10	26	theme	caries	1504:1509	arg1	development					1489:1499	the development	1485:1499	the development of caries	1485:1509	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	1	27	from	shifts	119:124	arg1	biofilms					150:157	dental biofilms	143:157	dental biofilms	143:157	Caries is associated with shifts of microbiota in dental biofilms and primarily driven by frequent sucrose consumption.					
30242260	10	28	from	changes	1287:1293	arg1	microbiota					1302:1311	the microbiota	1298:1311	the microbiota	1298:1311	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	0	29	theme	In-vivo	0:6	arg1	shift					8:12	In-vivo shift	0:12	In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.	0:91	In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.					
30242260	4	30	theme	sucrose	546:552	arg1	10 g					538:541	sucking 10 g	530:541	sucking 10 g of sucrose per day	530:560	After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase.					
30242260	3	31	contain	containing	390:399	arg1	systems					382:388	Splint systems	375:388	Splint systems containing enamel slabs	375:412	Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples.					
30242260	3	31	contain	containing	390:399	arg2	slabs					408:412	enamel slabs	401:412	enamel slabs	401:412	Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples.					
30242260	10	32	theme	non-mutans	1340:1349	arg1	streptococci					1351:1362	non-mutans streptococci	1340:1362	non-mutans streptococci	1340:1362	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	10	33	dep	provoked	1264:1271	arg1	emphasizing					1428:1438	emphasizing	1428:1438	emphasizing the synergy of multiple bacterial species in the development of caries	1428:1509	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	10	33	dep	provoked	1264:1271	arg1	supporting					1371:1380	supporting	1371:1380	supporting the extended ecological plaque hypothesis	1371:1422	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	7	34	from	differences	871:881	arg1	beta-diversity					886:899	beta-diversity	886:899	beta-diversity	886:899	The sucrose phase resulted in significant differences in beta-diversity and significantly decreased species richness.					
30242260	9	35	theme	Enamel	1111:1116	arg1	roughness					1126:1134	Enamel surface roughness	1111:1134	Enamel surface roughness	1111:1134	Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface.					
30242260	3	36	theme	Splint	375:380	arg1	systems					382:388	Splint systems	375:388	Splint systems containing enamel slabs	375:412	Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples.					
30242260	9	37	theme	surface	1118:1124	arg1	roughness					1126:1134	Enamel surface roughness	1111:1134	Enamel surface roughness	1111:1134	Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface.					
30242260	1	38	theme	frequent	183:190	arg1	consumption					200:210	frequent sucrose consumption	183:210	frequent sucrose consumption	183:210	Caries is associated with shifts of microbiota in dental biofilms and primarily driven by frequent sucrose consumption.					
30242260	10	39	theme	multiple	1455:1462	arg1	species					1474:1480	multiple bacterial species	1455:1480	multiple bacterial species	1455:1480	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	3	40	theme	7-day	444:448	arg1	intervals					450:458	7-day intervals	444:458	7-day intervals	444:458	Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples.					
30242260	10	41	theme	frequent	1235:1242	arg1	consumption					1252:1262	frequent sucrose consumption	1235:1262	frequent sucrose consumption	1235:1262	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	1	42	theme	sucrose	192:198	arg1	consumption					200:210	frequent sucrose consumption	183:210	frequent sucrose consumption	183:210	Caries is associated with shifts of microbiota in dental biofilms and primarily driven by frequent sucrose consumption.					
30242260	0	43	theme	microbiota	21:30	arg1	shift					8:12	In-vivo shift	0:12	In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.	0:91	In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.					
30242260	10	44	theme	ecological	1395:1404	arg1	hypothesis					1413:1422	the extended ecological plaque hypothesis	1382:1422	the extended ecological plaque hypothesis	1382:1422	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	6	45	theme	scanning	808:815	arg1	microscopy					817:826	laser scanning microscopy	802:826	laser scanning microscopy	802:826	In addition, roughness of the enamel surface was measured with laser scanning microscopy.					
30242260	4	46	theme	dietary	512:518	arg1	change					520:525	a three-month dietary change	498:525	a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet	498:592	After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase.					
30242260	3	47	theme	enamel	401:406	arg1	slabs					408:412	enamel slabs	401:412	enamel slabs	401:412	Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples.					
30242260	9	48	theme	surface	1202:1208	arg1	impairment					1174:1183	initial impairment	1166:1183	initial impairment of dental enamel surface	1166:1208	Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface.					
30242260	6	49	theme	laser	802:806	arg1	microscopy					817:826	laser scanning microscopy	802:826	laser scanning microscopy	802:826	In addition, roughness of the enamel surface was measured with laser scanning microscopy.					
30242260	2	50	theme	consumption	342:352	arg1	influence					312:320	the influence	308:320	the influence of frequent sucrose consumption on the oral biofilm	308:372	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	0	51	theme	oral	35:38	arg1	biofilm					40:46	oral biofilm	35:46	oral biofilm in response to frequent sucrose consumption	35:90	In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.					
30242260	4	52	theme	10 g	538:541	arg1	change					520:525	a three-month dietary change	498:525	a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet	498:592	After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase.					
30242260	2	53	theme	sucrose	334:340	arg1	consumption					342:352	frequent sucrose consumption	325:352	frequent sucrose consumption	325:352	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	4	54	theme	sucking	530:536	arg1	10 g					538:541	sucking 10 g	530:541	sucking 10 g of sucrose per day	530:560	After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase.					
30242260	3	55	with	days	434:437	arg1	intervals					450:458	7-day intervals	444:458	7-day intervals	444:458	Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples.					
30242260	2	56	theme	microbiota	253:262	arg1	shifts					264:269	environmentally induced in vivo microbiota shifts	221:269	environmentally induced in vivo microbiota shifts	221:269	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	4	57	theme	second	640:645	arg1	phase					647:651	the second phase	636:651	the second phase	636:651	After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase.					
30242260	2	58	theme	frequent	325:332	arg1	consumption					342:352	frequent sucrose consumption	325:352	frequent sucrose consumption	325:352	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	10	59	theme	streptococci	1351:1362	arg1	increase					1328:1335	an increase	1325:1335	an increase of non-mutans streptococci	1325:1362	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	10	60	theme	plaque	1406:1411	arg1	hypothesis					1413:1422	the extended ecological plaque hypothesis	1382:1422	the extended ecological plaque hypothesis	1382:1422	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	1	61	theme	microbiota	129:138	arg1	shifts					119:124	shifts	119:124	shifts of microbiota in dental biofilms	119:157	Caries is associated with shifts of microbiota in dental biofilms and primarily driven by frequent sucrose consumption.					
30242260	10	62	theme	sucrose	1244:1250	arg1	consumption					1252:1262	frequent sucrose consumption	1235:1262	frequent sucrose consumption	1235:1262	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	3	63	theme	oral	470:473	arg1	samples					483:489	oral biofilm samples	470:489	oral biofilm samples	470:489	Splint systems containing enamel slabs were worn for 3 × 7 days with 7-day intervals to obtain oral biofilm samples.					
30242260	6	64	theme	surface	776:782	arg1	roughness					752:760	roughness	752:760	roughness of the enamel surface	752:782	In addition, roughness of the enamel surface was measured with laser scanning microscopy.					
30242260	9	65	theme	initial	1166:1172	arg1	impairment					1174:1183	initial impairment	1166:1183	initial impairment of dental enamel surface	1166:1208	Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface.					
30242260	4	66	theme	phase	647:651	arg1	end					629:631	the end	625:631	the end of the second phase	625:651	After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase.					
30242260	7	67	theme	significant	859:869	arg1	differences					871:881	significant differences	859:881	significant differences in beta-diversity	859:899	The sucrose phase resulted in significant differences in beta-diversity and significantly decreased species richness.					
30242260	5	68	theme	v1-v2	724:728	arg1	sequencing					712:721	Illumina MiSeq amplicon sequencing	688:721	Illumina MiSeq amplicon sequencing (v1-v2 region)	688:736	The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region).					
30242260	5	68	theme	v1-v2	724:728	arg1	region					730:735	v1-v2 region	724:735	v1-v2 region	724:735	The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region).					
30242260	2	69	theme	induced	237:243	arg1	shifts					264:269	environmentally induced in vivo microbiota shifts	221:269	environmentally induced in vivo microbiota shifts	221:269	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	10	70	theme	extended	1386:1393	arg1	hypothesis					1413:1422	the extended ecological plaque hypothesis	1382:1422	the extended ecological plaque hypothesis	1382:1422	The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.					
30242260	2	71	theme	oral	361:364	arg1	biofilm					366:372	the oral biofilm	357:372	the oral biofilm	357:372	Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm.					
30242260	5	72	theme	MiSeq	697:701	arg1	sequencing					712:721	Illumina MiSeq amplicon sequencing	688:721	Illumina MiSeq amplicon sequencing (v1-v2 region)	688:736	The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region).					
30242260	5	72	theme	MiSeq	697:701	arg1	region					730:735	v1-v2 region	724:735	v1-v2 region	724:735	The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region).					
31324524	1	0	theme	infectious	290:299	arg1	disease					301:307	the single most widespread and deadly infectious disease	252:307	the single most widespread and deadly infectious disease	252:307	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	3	1	theme	bacterial	445:453	arg1	factors					455:461	bacterial factors	445:461	bacterial factors	445:461	Nonetheless, the participation of bacterial factors is becoming increasingly evident.					
31324524	1	2	theme	history	216:222	arg1	TB					240:241	TB	240:241	TB	240:241	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	1	2	theme	history	216:222	arg1	tuberculosis					226:237	history ̶ tuberculosis	216:237	history ̶ tuberculosis (TB)	216:242	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	9	3	theme	α-glucans	1385:1393	arg1	role					1377:1380	a relevant role	1366:1380	a relevant role of α-glucans for the onset of the protective immune response against TB	1366:1452	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	3	4	theme	factors	455:461	arg1	participation					428:440	the participation	424:440	the participation of bacterial factors	424:461	Nonetheless, the participation of bacterial factors is becoming increasingly evident.					
31324524	4	5	theme	Minimal	497:503	arg1	variations					505:514	Minimal variations	497:514	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis	497:594	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis can lead to marked differences in immunogenicity.					
31324524	1	6	theme	̶	224:224	arg1	TB					240:241	TB	240:241	TB	240:241	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	1	6	theme	̶	224:224	arg1	tuberculosis					226:237	history ̶ tuberculosis	216:237	history ̶ tuberculosis (TB)	216:242	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	5	7	theme	antigen	684:690	arg1	cells					703:707	professional antigen presenting cells	671:707	professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb	671:866	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	7	theme	antigen	684:690	arg1	cells					656:660	Dendritic cells	646:660	Dendritic cells (DC)	646:665	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	0	8	from	Role	0:3	arg1	response					84:91	immune response	77:91	immune response	77:91	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	0	8	from	Role	0:3	arg1	cells					53:57	dendritic cells	43:57	dendritic cells	43:57	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	6	9	from	impact	986:991	arg1	DC					996:997	DC maturation and function	996:1021	DC maturation and function	996:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	9	from	impact	986:991	arg1	function					1014:1021	function	1014:1021	function	1014:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	9	from	impact	986:991	arg1	maturation					999:1008	maturation	999:1008	maturation	999:1008	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	10	theme	endogenous	923:932	arg1	production					934:943	the endogenous production	919:943	the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function	919:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	0	11	theme	immune	77:82	arg1	response					84:91	immune response	77:91	immune response	77:91	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	9	12	theme	response	1434:1441	arg1	onset					1403:1407	the onset	1399:1407	the onset of the protective immune response against TB	1399:1452	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	6	13	from	species	964:970	arg1	DC					996:997	DC maturation and function	996:1021	DC maturation and function	996:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	13	from	species	964:970	arg1	function					1014:1021	function	1014:1021	function	1014:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	13	from	species	964:970	arg1	maturation					999:1008	maturation	999:1008	maturation	999:1008	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	8	14	from	phagocytosis	1222:1233	arg1	essential					1195:1203	essential	1195:1203	essential	1195:1203	Even more, α-glucans induced Syk activation but were not essential in non-opsonized phagocytosis.					
31324524	5	15	theme	response	847:854	arg1	onset					827:831	the onset	823:831	the onset of the immune response against Mtb	823:866	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	6	16	theme	reactive	948:955	arg1	species					964:970	reactive oxygen species	948:970	reactive oxygen species	948:970	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	5	17	theme	strain	797:802	arg1	critical					810:817	critical	810:817	critical	810:817	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	17	theme	strain	797:802	arg1	composition					762:772	the cell wall composition	748:772	the cell wall composition of each particular Mtb strain	748:802	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	17	theme	strain	797:802	arg1	depending					735:743	depending	735:743	depending	735:743	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	1	18	theme	million	151:157	arg1	deaths					159:164	three million deaths	145:164	three million deaths	145:164	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	0	19	theme	dendritic	43:51	arg1	cells					53:57	dendritic cells	43:57	dendritic cells	43:57	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	4	20	theme	Mycobacterium	536:548	arg1	tuberculosis					550:561	Mycobacterium tuberculosis	536:561	Mycobacterium tuberculosis (Mtb) virulence and pathogenesis	536:594	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis can lead to marked differences in immunogenicity.					
31324524	4	20	theme	Mycobacterium	536:548	arg1	Mtb					564:566	Mtb	564:566	Mtb	564:566	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis can lead to marked differences in immunogenicity.					
31324524	9	21	theme	protective	1416:1425	arg1	response					1434:1441	the protective immune response	1412:1441	the protective immune response against TB	1412:1452	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	4	22	from	differences	615:625	arg1	immunogenicity					630:643	immunogenicity	630:643	immunogenicity	630:643	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis can lead to marked differences in immunogenicity.					
31324524	4	23	theme	marked	608:613	arg1	differences					615:625	marked differences	608:625	marked differences in immunogenicity	608:643	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis can lead to marked differences in immunogenicity.					
31324524	7	24	theme	DC	1094:1095	arg1	maturation					1097:1106	DC maturation	1094:1106	DC maturation	1094:1106	Results showed that α-glucans on Mtb induce ROS production leading to DC maturation and lymphocyte proliferation.					
31324524	5	25	dep	critical	810:817	arg1	critical					810:817	critical	810:817	critical	810:817	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	25	dep	critical	810:817	arg1	composition					762:772	the cell wall composition	748:772	the cell wall composition of each particular Mtb strain	748:802	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	25	dep	critical	810:817	arg1	depending					735:743	depending	735:743	depending	735:743	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	6	26	from	ROS	973:975	arg1	DC					996:997	DC maturation and function	996:1021	DC maturation and function	996:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	26	from	ROS	973:975	arg1	function					1014:1021	function	1014:1021	function	1014:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	26	from	ROS	973:975	arg1	maturation					999:1008	maturation	999:1008	maturation	999:1008	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	9	27	theme	Mtb	1261:1263	arg1	α-glucans					1248:1256	α-glucans	1248:1256	α-glucans of Mtb	1248:1263	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	6	28	from	production	934:943	arg1	DC					996:997	DC maturation and function	996:1021	DC maturation and function	996:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	28	from	production	934:943	arg1	function					1014:1021	function	1014:1021	function	1014:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	28	from	production	934:943	arg1	maturation					999:1008	maturation	999:1008	maturation	999:1008	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	4	29	dep	tuberculosis	550:561	arg1	pathogenesis					583:594	pathogenesis	583:594	pathogenesis	583:594	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis can lead to marked differences in immunogenicity.					
31324524	4	29	dep	tuberculosis	550:561	arg1	virulence					569:577	virulence	569:577	virulence	569:577	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis can lead to marked differences in immunogenicity.					
31324524	0	30	theme	oxygen	25:30	arg1	species					32:38	α-glucan-induced oxygen species	8:38	α-glucan-induced oxygen species	8:38	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	8	31	theme	Syk	1167:1169	arg1	activation					1171:1180	Syk activation	1167:1180	Syk activation	1167:1180	Even more, α-glucans induced Syk activation but were not essential in non-opsonized phagocytosis.					
31324524	6	32	from	DC	996:997	arg1	production					934:943	the endogenous production	919:943	the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function	919:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	7	33	from	α-glucans	1044:1052	arg1	Mtb					1057:1059	Mtb	1057:1059	Mtb	1057:1059	Results showed that α-glucans on Mtb induce ROS production leading to DC maturation and lymphocyte proliferation.					
31324524	5	34	theme	Mtb	793:795	arg1	strain					797:802	each particular Mtb strain	777:802	each particular Mtb strain	777:802	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	0	35	theme	α-glucan-induced	8:23	arg1	species					32:38	α-glucan-induced oxygen species	8:38	α-glucan-induced oxygen species	8:38	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	4	36	theme	related	525:531	arg1	genes					519:523	genes	519:523	genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis	519:594	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis can lead to marked differences in immunogenicity.					
31324524	8	37	theme	more	1143:1146	arg1	α-glucans					1149:1157	Even more, α-glucans	1138:1157	α-glucans	1149:1157	Even more, α-glucans induced Syk activation but were not essential in non-opsonized phagocytosis.					
31324524	6	38	dep	DC	996:997	arg1	DC					996:997	DC maturation and function	996:1021	DC maturation and function	996:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	38	dep	DC	996:997	arg1	function					1014:1021	function	1014:1021	function	1014:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	38	dep	DC	996:997	arg1	maturation					999:1008	maturation	999:1008	maturation	999:1008	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	2	39	theme	active	362:367	arg1	TB					369:370	both latent and active TB	346:370	both latent and active TB	346:370	Until recently, it was thought that both latent and active TB was primarily related to host factors.					
31324524	7	40	theme	ROS	1068:1070	arg1	production					1072:1081	ROS production	1068:1081	ROS production	1068:1081	Results showed that α-glucans on Mtb induce ROS production leading to DC maturation and lymphocyte proliferation.					
31324524	9	41	theme	ROS	1281:1283	arg1	production					1285:1294	ROS production	1281:1294	ROS production	1281:1294	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	0	42	from	impact	67:72	arg1	response					84:91	immune response	77:91	immune response	77:91	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	0	42	from	impact	67:72	arg1	cells					53:57	dendritic cells	43:57	dendritic cells	43:57	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	6	43	theme	impact	986:991	arg1	production					934:943	the endogenous production	919:943	the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function	919:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	0	44	theme	species	32:38	arg1	impact					67:72	its impact	63:72	its impact in immune response against tuberculosis	63:112	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	0	44	theme	species	32:38	arg1	Role					0:3	Role	0:3	Role of α-glucan-induced oxygen species in dendritic cells	0:57	Role of α-glucan-induced oxygen species in dendritic cells and its impact in immune response against tuberculosis.					
31324524	1	45	theme	single	256:261	arg1	disease					301:307	the single most widespread and deadly infectious disease	252:307	the single most widespread and deadly infectious disease	252:307	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	5	46	theme	professional	671:682	arg1	cells					703:707	professional antigen presenting cells	671:707	professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb	671:866	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	46	theme	professional	671:682	arg1	cells					656:660	Dendritic cells	646:660	Dendritic cells (DC)	646:665	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	7	47	theme	lymphocyte	1112:1121	arg1	proliferation					1123:1135	lymphocyte proliferation	1112:1135	lymphocyte proliferation	1112:1135	Results showed that α-glucans on Mtb induce ROS production leading to DC maturation and lymphocyte proliferation.					
31324524	2	48	theme	latent	351:356	arg1	TB					369:370	both latent and active TB	346:370	both latent and active TB	346:370	Until recently, it was thought that both latent and active TB was primarily related to host factors.					
31324524	5	49	theme	cell	752:755	arg1	critical					810:817	critical	810:817	critical	810:817	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	49	theme	cell	752:755	arg1	composition					762:772	the cell wall composition	748:772	the cell wall composition of each particular Mtb strain	748:802	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	49	theme	cell	752:755	arg1	depending					735:743	depending	735:743	depending	735:743	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	50	theme	wall	757:760	arg1	critical					810:817	critical	810:817	critical	810:817	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	50	theme	wall	757:760	arg1	composition					762:772	the cell wall composition	748:772	the cell wall composition of each particular Mtb strain	748:802	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	50	theme	wall	757:760	arg1	depending					735:743	depending	735:743	depending	735:743	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	9	51	theme	relevant	1368:1375	arg1	role					1377:1380	a relevant role	1366:1380	a relevant role of α-glucans for the onset of the protective immune response against TB	1366:1452	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	2	52	theme	host	397:400	arg1	factors					402:408	host factors	397:408	host factors	397:408	Until recently, it was thought that both latent and active TB was primarily related to host factors.					
31324524	1	53	theme	widespread	268:277	arg1	disease					301:307	the single most widespread and deadly infectious disease	252:307	the single most widespread and deadly infectious disease	252:307	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	5	54	theme	Dendritic	646:654	arg1	cells					656:660	Dendritic cells	646:660	Dendritic cells (DC)	646:665	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	54	theme	Dendritic	646:654	arg1	cells					703:707	professional antigen presenting cells	671:707	professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb	671:866	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	54	theme	Dendritic	646:654	arg1	DC					663:664	DC	663:664	DC	663:664	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	8	55	theme	non-opsonized	1208:1220	arg1	phagocytosis					1222:1233	non-opsonized phagocytosis	1208:1233	non-opsonized phagocytosis	1208:1233	Even more, α-glucans induced Syk activation but were not essential in non-opsonized phagocytosis.					
31324524	9	56	theme	subsequent	1304:1313	arg1	maturation					1318:1327	the subsequent DC maturation	1300:1327	the subsequent DC maturation	1300:1327	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	9	57	theme	immune	1427:1432	arg1	response					1434:1441	the protective immune response	1412:1441	the protective immune response against TB	1412:1452	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	9	58	theme	DC	1315:1316	arg1	maturation					1318:1327	the subsequent DC maturation	1300:1327	the subsequent DC maturation	1300:1327	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	5	59	theme	particular	782:791	arg1	strain					797:802	each particular Mtb strain	777:802	each particular Mtb strain	777:802	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	1	60	theme	million	123:129	arg1	cases					135:139	10 million new cases	120:139	10 million new cases	120:139	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	6	61	theme	species	964:970	arg1	production					934:943	the endogenous production	919:943	the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function	919:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	6	62	theme	ROS	973:975	arg1	production					934:943	the endogenous production	919:943	the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function	919:1021	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	1	63	dep	occur	179:183	arg1	̶					192:192	̶	192:192	to occur yearly ̶ more than any time in history ̶ tuberculosis (TB)	176:242	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	8	64	from	essential	1195:1203	arg1	phagocytosis					1222:1233	non-opsonized phagocytosis	1208:1233	non-opsonized phagocytosis	1208:1233	Even more, α-glucans induced Syk activation but were not essential in non-opsonized phagocytosis.					
31324524	1	65	theme	new	131:133	arg1	cases					135:139	10 million new cases	120:139	10 million new cases	120:139	With 10 million new cases and three million deaths estimated to occur yearly ̶ more than any time in history ̶ tuberculosis (TB) remains the single most widespread and deadly infectious disease.					
31324524	6	66	theme	oxygen	957:962	arg1	species					964:970	reactive oxygen species	948:970	reactive oxygen species	948:970	Here we evaluated the role played by α-glucan, in the endogenous production of reactive oxygen species, ROS, and the impact on DC maturation and function.					
31324524	4	67	from	variations	505:514	arg1	genes					519:523	genes	519:523	genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis	519:594	Minimal variations in genes related to Mycobacterium tuberculosis (Mtb) virulence and pathogenesis can lead to marked differences in immunogenicity.					
31324524	5	68	theme	immune	840:845	arg1	response					847:854	the immune response	836:854	the immune response against Mtb	836:866	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	9	69	theme	antigen	1333:1339	arg1	presentation					1341:1352	antigen presentation	1333:1352	antigen presentation	1333:1352	In summary, α-glucans of Mtb participates in ROS production and the subsequent DC maturation and antigen presentation, suggesting a relevant role of α-glucans for the onset of the protective immune response against TB.					
31324524	5	70	theme	presenting	692:701	arg1	cells					703:707	professional antigen presenting cells	671:707	professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb	671:866	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31324524	5	70	theme	presenting	692:701	arg1	cells					656:660	Dendritic cells	646:660	Dendritic cells (DC)	646:665	Dendritic cells (DC) are professional antigen presenting cells whose maturation can vary depending on the cell wall composition of each particular Mtb strain being critical for the onset of the immune response against Mtb.					
31739020	6	0	from	change	702:707	arg1	structure					722:730	secondary structure	712:730	secondary structure of zein	712:738	The incorporation of DS led to the change in secondary structure of zein.					
31739020	1	1	theme	limited	151:157	arg1	use					159:161	limited use	151:161	limited use	151:161	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	1	1	theme	limited	151:157	arg1	result					168:173	a result	166:173	a result of its poor colloidal stability	166:205	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	3	2	theme	composite	322:330	arg1	ZDSNPs					347:352	ZDSNPs	347:352	ZDSNPs	347:352	Zein/DS composite nanoparticles (ZDSNPs) were fabricated by antisolvent precipitation method at pH 4.0 with an optimal zein-to-DS ratio of 1:2 (w/w).					
31739020	3	2	theme	composite	322:330	arg1	nanoparticles					332:344	Zein/DS composite nanoparticles	314:344	Zein/DS composite nanoparticles (ZDSNPs)	314:353	Zein/DS composite nanoparticles (ZDSNPs) were fabricated by antisolvent precipitation method at pH 4.0 with an optimal zein-to-DS ratio of 1:2 (w/w).					
31739020	10	3	theme	encapsulation	968:980	arg1	%					1038:1038	85.37%	1033:1038	85.37%	1033:1038	The encapsulation efficiency of curcumin-loaded ZDSNPs (ZDSCNPs) was 85.37%, which was significantly higher than that of curcumin-loaded ZNPs.					
31739020	10	3	theme	encapsulation	968:980	arg1	efficiency					982:991	The encapsulation efficiency	964:991	The encapsulation efficiency of curcumin-loaded ZDSNPs (ZDSCNPs)	964:1027	The encapsulation efficiency of curcumin-loaded ZDSNPs (ZDSCNPs) was 85.37%, which was significantly higher than that of curcumin-loaded ZNPs.					
31739020	5	4	theme	hydrogen	620:627	arg1	attractions					607:617	electrostatic attractions	593:617	electrostatic attractions	593:617	Additionally, ZDSNPs showed spherical structures formed primarily by electrostatic attractions, hydrogen bonding and hydrophobic interactions.					
31739020	5	4	theme	hydrogen	620:627	arg1	bonding					629:635	hydrogen bonding	620:635	hydrogen bonding	620:635	Additionally, ZDSNPs showed spherical structures formed primarily by electrostatic attractions, hydrogen bonding and hydrophobic interactions.					
31739020	11	5	theme	storage	1147:1153	arg1	stability					1155:1163	improved storage stability	1138:1163	improved storage stability	1138:1163	In addition, ZDSCNPs exhibited improved storage stability and bioaccessibility of curcumin.					
31739020	5	6	theme	spherical	552:560	arg1	structures					562:571	spherical structures	552:571	spherical structures formed primarily by electrostatic attractions, hydrogen bonding and hydrophobic interactions	552:664	Additionally, ZDSNPs showed spherical structures formed primarily by electrostatic attractions, hydrogen bonding and hydrophobic interactions.					
31739020	6	7	theme	DS	688:689	arg1	incorporation					671:683	The incorporation	667:683	The incorporation of DS	667:689	The incorporation of DS led to the change in secondary structure of zein.					
31739020	0	8	theme	curcumin	110:117	arg1	delivery					98:105	delivery	98:105	delivery of curcumin	98:117	Fabrication and characterization of zein nanoparticles by dextran sulfate coating as vehicles for delivery of curcumin.					
31739020	1	9	contain	have	146:149	arg1	nanoparticles					125:137	Zein nanoparticles	120:137	Zein nanoparticles (ZNPs)	120:144	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	1	9	contain	have	146:149	arg2	use					159:161	limited use	151:161	limited use	151:161	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	1	9	contain	have	146:149	arg2	result					168:173	a result	166:173	a result of its poor colloidal stability	166:205	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	1	9	contain	have	146:149	arg1	ZNPs					140:143	ZNPs	140:143	ZNPs	140:143	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	11	10	theme	improved	1138:1145	arg1	stability					1155:1163	improved storage stability	1138:1163	improved storage stability	1138:1163	In addition, ZDSCNPs exhibited improved storage stability and bioaccessibility of curcumin.					
31739020	2	11	theme	polysaccharide-dextran	277:298	arg1	DS					309:310	DS	309:310	DS	309:310	The goal of this paper is to stabilize ZNPs using a sulfated anionic polysaccharide-dextran sulfate (DS).					
31739020	2	11	theme	polysaccharide-dextran	277:298	arg1	sulfate					300:306	a sulfated anionic polysaccharide-dextran sulfate	258:306	a sulfated anionic polysaccharide-dextran sulfate (DS)	258:311	The goal of this paper is to stabilize ZNPs using a sulfated anionic polysaccharide-dextran sulfate (DS).					
31739020	7	12	theme	DS	811:812	arg1	deposition					797:806	the deposition	793:806	the deposition of DS	793:812	The surface hydrophobicity of ZNPs reduced owing to the deposition of DS.					
31739020	7	13	theme	ZNPs	771:774	arg1	hydrophobicity					753:766	The surface hydrophobicity	741:766	The surface hydrophobicity of ZNPs	741:774	The surface hydrophobicity of ZNPs reduced owing to the deposition of DS.					
31739020	5	14	theme	electrostatic	593:605	arg1	interactions					653:664	hydrophobic interactions	641:664	hydrophobic interactions	641:664	Additionally, ZDSNPs showed spherical structures formed primarily by electrostatic attractions, hydrogen bonding and hydrophobic interactions.					
31739020	5	14	theme	electrostatic	593:605	arg1	attractions					607:617	electrostatic attractions	593:617	electrostatic attractions	593:617	Additionally, ZDSNPs showed spherical structures formed primarily by electrostatic attractions, hydrogen bonding and hydrophobic interactions.					
31739020	5	14	theme	electrostatic	593:605	arg1	bonding					629:635	hydrogen bonding	620:635	hydrogen bonding	620:635	Additionally, ZDSNPs showed spherical structures formed primarily by electrostatic attractions, hydrogen bonding and hydrophobic interactions.					
31739020	2	15	theme	anionic	269:275	arg1	DS					309:310	DS	309:310	DS	309:310	The goal of this paper is to stabilize ZNPs using a sulfated anionic polysaccharide-dextran sulfate (DS).					
31739020	2	15	theme	anionic	269:275	arg1	sulfate					300:306	a sulfated anionic polysaccharide-dextran sulfate	258:306	a sulfated anionic polysaccharide-dextran sulfate (DS)	258:311	The goal of this paper is to stabilize ZNPs using a sulfated anionic polysaccharide-dextran sulfate (DS).					
31739020	8	16	theme	normal	853:858	arg1	lines					902:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	And ZDSNPs were basically nontoxic to normal colonic epithelial cell lines-NCM460 cell lines.					
31739020	6	17	theme	zein	735:738	arg1	structure					722:730	secondary structure	712:730	secondary structure of zein	712:738	The incorporation of DS led to the change in secondary structure of zein.					
31739020	2	18	theme	sulfated	260:267	arg1	DS					309:310	DS	309:310	DS	309:310	The goal of this paper is to stabilize ZNPs using a sulfated anionic polysaccharide-dextran sulfate (DS).					
31739020	2	18	theme	sulfated	260:267	arg1	sulfate					300:306	a sulfated anionic polysaccharide-dextran sulfate	258:306	a sulfated anionic polysaccharide-dextran sulfate (DS)	258:311	The goal of this paper is to stabilize ZNPs using a sulfated anionic polysaccharide-dextran sulfate (DS).					
31739020	3	19	theme	optimal	425:431	arg1	ratio					444:448	an optimal zein-to-DS ratio	422:448	an optimal zein-to-DS ratio of 1:2 (w/w)	422:461	Zein/DS composite nanoparticles (ZDSNPs) were fabricated by antisolvent precipitation method at pH 4.0 with an optimal zein-to-DS ratio of 1:2 (w/w).					
31739020	11	20	theme	curcumin	1189:1196	arg1	bioaccessibility					1169:1184	bioaccessibility	1169:1184	bioaccessibility of curcumin	1169:1196	In addition, ZDSCNPs exhibited improved storage stability and bioaccessibility of curcumin.					
31739020	11	20	theme	curcumin	1189:1196	arg1	stability					1155:1163	improved storage stability	1138:1163	improved storage stability	1138:1163	In addition, ZDSCNPs exhibited improved storage stability and bioaccessibility of curcumin.					
31739020	10	21	theme	curcumin-loaded	1085:1099	arg1	ZNPs					1101:1104	curcumin-loaded ZNPs	1085:1104	curcumin-loaded ZNPs	1085:1104	The encapsulation efficiency of curcumin-loaded ZDSNPs (ZDSCNPs) was 85.37%, which was significantly higher than that of curcumin-loaded ZNPs.					
31739020	8	22	theme	epithelial	868:877	arg1	lines					902:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	And ZDSNPs were basically nontoxic to normal colonic epithelial cell lines-NCM460 cell lines.					
31739020	3	23	theme	zein-to-DS	433:442	arg1	ratio					444:448	an optimal zein-to-DS ratio	422:448	an optimal zein-to-DS ratio of 1:2 (w/w)	422:461	Zein/DS composite nanoparticles (ZDSNPs) were fabricated by antisolvent precipitation method at pH 4.0 with an optimal zein-to-DS ratio of 1:2 (w/w).					
31739020	3	24	theme	antisolvent	374:384	arg1	method					400:405	antisolvent precipitation method	374:405	antisolvent precipitation method	374:405	Zein/DS composite nanoparticles (ZDSNPs) were fabricated by antisolvent precipitation method at pH 4.0 with an optimal zein-to-DS ratio of 1:2 (w/w).					
31739020	10	25	theme	ZDSNPs	1012:1017	arg1	%					1038:1038	85.37%	1033:1038	85.37%	1033:1038	The encapsulation efficiency of curcumin-loaded ZDSNPs (ZDSCNPs) was 85.37%, which was significantly higher than that of curcumin-loaded ZNPs.					
31739020	10	25	theme	ZDSNPs	1012:1017	arg1	efficiency					982:991	The encapsulation efficiency	964:991	The encapsulation efficiency of curcumin-loaded ZDSNPs (ZDSCNPs)	964:1027	The encapsulation efficiency of curcumin-loaded ZDSNPs (ZDSCNPs) was 85.37%, which was significantly higher than that of curcumin-loaded ZNPs.					
31739020	8	26	theme	colonic	860:866	arg1	lines					902:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	And ZDSNPs were basically nontoxic to normal colonic epithelial cell lines-NCM460 cell lines.					
31739020	1	27	theme	poor	182:185	arg1	stability					197:205	its poor colloidal stability	178:205	its poor colloidal stability	178:205	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	0	28	theme	zein	36:39	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of zein nanoparticles by dextran sulfate coating as vehicles for delivery of curcumin.					
31739020	0	28	theme	zein	36:39	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of zein nanoparticles by dextran sulfate coating as vehicles for delivery of curcumin.					
31739020	4	29	theme	good	481:484	arg1	stability					486:494	good stability	481:494	good stability	481:494	ZDSNPs exhibited good stability to pH, heating and storage.					
31739020	3	30	theme	precipitation	386:398	arg1	method					400:405	antisolvent precipitation method	374:405	antisolvent precipitation method	374:405	Zein/DS composite nanoparticles (ZDSNPs) were fabricated by antisolvent precipitation method at pH 4.0 with an optimal zein-to-DS ratio of 1:2 (w/w).					
31739020	10	31	theme	curcumin-loaded	996:1010	arg1	ZDSNPs					1012:1017	curcumin-loaded ZDSNPs	996:1017	curcumin-loaded ZDSNPs (ZDSCNPs)	996:1027	The encapsulation efficiency of curcumin-loaded ZDSNPs (ZDSCNPs) was 85.37%, which was significantly higher than that of curcumin-loaded ZNPs.					
31739020	10	31	theme	curcumin-loaded	996:1010	arg1	ZDSCNPs					1020:1026	ZDSCNPs	1020:1026	ZDSCNPs	1020:1026	The encapsulation efficiency of curcumin-loaded ZDSNPs (ZDSCNPs) was 85.37%, which was significantly higher than that of curcumin-loaded ZNPs.					
31739020	8	32	theme	lines-NCM460	884:895	arg1	lines					902:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	And ZDSNPs were basically nontoxic to normal colonic epithelial cell lines-NCM460 cell lines.					
31739020	1	33	theme	colloidal	187:195	arg1	stability					197:205	its poor colloidal stability	178:205	its poor colloidal stability	178:205	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	0	34	theme	dextran	58:64	arg1	sulfate					66:72	dextran sulfate	58:72	dextran sulfate coating as vehicles for delivery of curcumin	58:117	Fabrication and characterization of zein nanoparticles by dextran sulfate coating as vehicles for delivery of curcumin.					
31739020	7	35	theme	surface	745:751	arg1	hydrophobicity					753:766	The surface hydrophobicity	741:766	The surface hydrophobicity of ZNPs	741:774	The surface hydrophobicity of ZNPs reduced owing to the deposition of DS.					
31739020	8	36	theme	cell	879:882	arg1	lines					902:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	And ZDSNPs were basically nontoxic to normal colonic epithelial cell lines-NCM460 cell lines.					
31739020	3	37	theme	1:2	453:455	arg1	ratio					444:448	an optimal zein-to-DS ratio	422:448	an optimal zein-to-DS ratio of 1:2 (w/w)	422:461	Zein/DS composite nanoparticles (ZDSNPs) were fabricated by antisolvent precipitation method at pH 4.0 with an optimal zein-to-DS ratio of 1:2 (w/w).					
31739020	5	38	theme	hydrophobic	641:651	arg1	interactions					653:664	hydrophobic interactions	641:664	hydrophobic interactions	641:664	Additionally, ZDSNPs showed spherical structures formed primarily by electrostatic attractions, hydrogen bonding and hydrophobic interactions.					
31739020	5	38	theme	hydrophobic	641:651	arg1	attractions					607:617	electrostatic attractions	593:617	electrostatic attractions	593:617	Additionally, ZDSNPs showed spherical structures formed primarily by electrostatic attractions, hydrogen bonding and hydrophobic interactions.					
31739020	6	39	theme	secondary	712:720	arg1	structure					722:730	secondary structure	712:730	secondary structure of zein	712:738	The incorporation of DS led to the change in secondary structure of zein.					
31739020	1	40	theme	Zein	120:123	arg1	nanoparticles					125:137	Zein nanoparticles	120:137	Zein nanoparticles (ZNPs)	120:144	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	1	40	theme	Zein	120:123	arg1	ZNPs					140:143	ZNPs	140:143	ZNPs	140:143	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	9	41	used	used	934:937	arg2	ZDSNPs					909:914	ZDSNPs	909:914	ZDSNPs	909:914	ZDSNPs were successfully used to encapsulate curcumin.					
31739020	8	42	theme	cell	897:900	arg1	lines					902:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	normal colonic epithelial cell lines-NCM460 cell lines	853:906	And ZDSNPs were basically nontoxic to normal colonic epithelial cell lines-NCM460 cell lines.					
31739020	1	43	theme	stability	197:205	arg1	use					159:161	limited use	151:161	limited use	151:161	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	1	43	theme	stability	197:205	arg1	result					168:173	a result	166:173	a result of its poor colloidal stability	166:205	Zein nanoparticles (ZNPs) have limited use as a result of its poor colloidal stability.					
31739020	2	44	theme	paper	225:229	arg1	goal					212:215	The goal	208:215	The goal of this paper	208:229	The goal of this paper is to stabilize ZNPs using a sulfated anionic polysaccharide-dextran sulfate (DS).					
30708013	0	0	theme	In	102:103	arg1	assessment					123:132	In vitro and in vivo assessment	102:132	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.	0:133	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.					
30708013	8	1	used	used	1339:1342	arg2	hydrogels					1322:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	8	1	used	used	1339:1342	arg2	systems					1370:1376	promising drug release systems	1347:1376	promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment	1347:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	5	2	dep	in	820:821	arg1	vivo					823:826	vivo	823:826	vivo	823:826	In vitro and in vivo studies showed that the LMWH and PCL release was significantly affected by polymer molecular weight and block composition.					
30708013	1	3	theme	systemic	168:175	arg1	effects					183:189	systemic toxic effects	168:189	systemic toxic effects	168:189	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy are a promising alternative strategy.					
30708013	7	4	theme	plasma	1200:1205	arg1	concentration					1207:1219	the plasma concentration	1196:1219	the plasma concentration of LMWH released from aqueous solution	1196:1258	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	7	5	attach	released	1229:1236	arg2	concentration					1207:1219	the plasma concentration	1196:1219	the plasma concentration of LMWH released from aqueous solution	1196:1258	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	7	5	attach	released	1229:1236	arg1	solution					1251:1258	aqueous solution	1243:1258	aqueous solution	1243:1258	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	1	6	theme	toxic	177:181	arg1	effects					183:189	systemic toxic effects	168:189	systemic toxic effects	168:189	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy are a promising alternative strategy.					
30708013	5	7	theme	block	932:936	arg1	composition					938:948	block composition	932:948	block composition	932:948	In vitro and in vivo studies showed that the LMWH and PCL release was significantly affected by polymer molecular weight and block composition.					
30708013	8	8	theme	therapeutic	1424:1434	arg1	efficacy					1436:1443	therapeutic efficacy	1424:1443	therapeutic efficacy	1424:1443	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	4	9	theme	combined	770:777	arg1	treatment					786:794	a combined cancer treatment	768:794	a combined cancer treatment with LMWH	768:804	Paclitaxel (PCL) was co-encapsulated into the same cationic carrier for the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH.					
30708013	6	10	theme	larger	994:999	arg1	amounts					1001:1007	larger amounts	994:1007	larger amounts of LMWH	994:1015	Skin penetration tests have indicated that larger amounts of LMWH were absorbed from LMWH-gel conjugate through SC, than aqueous formula.					
30708013	6	10	theme	larger	994:999	arg1	LMWH					1012:1015	LMWH	1012:1015	LMWH	1012:1015	Skin penetration tests have indicated that larger amounts of LMWH were absorbed from LMWH-gel conjugate through SC, than aqueous formula.					
30708013	4	11	theme	therapeutic	743:753	arg1	efficacy					755:762	therapeutic efficacy	743:762	therapeutic efficacy	743:762	Paclitaxel (PCL) was co-encapsulated into the same cationic carrier for the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH.					
30708013	0	12	dep	in	115:116	arg1	vivo					118:121	vivo	118:121	vivo	118:121	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.					
30708013	8	13	theme	PCL-loaded	1284:1293	arg1	hydrogels					1322:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	8	13	theme	PCL-loaded	1284:1293	arg1	systems					1370:1376	promising drug release systems	1347:1376	promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment	1347:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	3	14	theme	heparin-conjugated	493:510	arg1	biomaterials					512:523	heparin-conjugated biomaterials	493:523	heparin-conjugated biomaterials	493:523	Recent studies have shown that heparin-conjugated biomaterials can be effective in inhibiting tumor growth during cancer treatment due to their high ability to bind growth factors.					
30708013	8	15	theme	drug	1357:1360	arg1	systems					1370:1376	promising drug release systems	1347:1376	promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment	1347:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	8	15	theme	drug	1357:1360	arg1	hydrogels					1322:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	5	16	theme	PCL	861:863	arg1	release					865:871	the LMWH and PCL release	848:871	the LMWH and PCL release	848:871	In vitro and in vivo studies showed that the LMWH and PCL release was significantly affected by polymer molecular weight and block composition.					
30708013	5	17	theme	LMWH	852:855	arg1	release					865:871	the LMWH and PCL release	848:871	the LMWH and PCL release	848:871	In vitro and in vivo studies showed that the LMWH and PCL release was significantly affected by polymer molecular weight and block composition.					
30708013	0	18	theme	in	115:116	arg1	assessment					123:132	In vitro and in vivo assessment	102:132	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.	0:133	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.					
30708013	5	19	dep	In	807:808	arg1	vitro					810:814	vitro	810:814	vitro	810:814	In vitro and in vivo studies showed that the LMWH and PCL release was significantly affected by polymer molecular weight and block composition.					
30708013	3	20	theme	tumor	556:560	arg1	growth					562:567	tumor growth	556:567	tumor growth	556:567	Recent studies have shown that heparin-conjugated biomaterials can be effective in inhibiting tumor growth during cancer treatment due to their high ability to bind growth factors.					
30708013	6	21	theme	aqueous	1072:1078	arg1	formula					1080:1086	aqueous formula	1072:1086	aqueous formula	1072:1086	Skin penetration tests have indicated that larger amounts of LMWH were absorbed from LMWH-gel conjugate through SC, than aqueous formula.					
30708013	7	22	theme	gel	1160:1162	arg1	conjugate					1164:1172	gel conjugate	1160:1172	gel conjugate	1160:1172	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	6	23	theme	penetration	956:966	arg1	tests					968:972	Skin penetration tests	951:972	Skin penetration tests	951:972	Skin penetration tests have indicated that larger amounts of LMWH were absorbed from LMWH-gel conjugate through SC, than aqueous formula.					
30708013	7	24	theme	aqueous	1243:1249	arg1	solution					1251:1258	aqueous solution	1243:1258	aqueous solution	1243:1258	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	0	25	dep	In	102:103	arg1	vitro					105:109	vitro	105:109	vitro	105:109	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.					
30708013	2	26	theme	stratum	388:394	arg1	corneum					396:402	the stratum corneum	384:402	the stratum corneum	384:402	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	2	27	theme	permeation	441:450	arg1	enhancer					452:459	a novel permeation enhancer	433:459	a novel permeation enhancer	433:459	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	2	27	theme	permeation	441:450	arg1	carrier					422:428	cationic carrier	413:428	cationic carrier	413:428	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	4	28	with	treatment	786:794	arg1	LMWH					801:804	LMWH	801:804	LMWH	801:804	Paclitaxel (PCL) was co-encapsulated into the same cationic carrier for the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH.					
30708013	8	29	theme	release	1362:1368	arg1	systems					1370:1376	promising drug release systems	1347:1376	promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment	1347:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	8	29	theme	release	1362:1368	arg1	hydrogels					1322:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	1	30	theme	therapeutic	208:218	arg1	efficacy					220:227	therapeutic efficacy	208:227	therapeutic efficacy	208:227	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy are a promising alternative strategy.					
30708013	0	31	theme	core/shell	30:39	arg1	hydrogels					41:49	LMWH-conjugated core/shell hydrogels	14:49	LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery	14:99	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.					
30708013	8	32	theme	LMWH-conjugated	1295:1309	arg1	hydrogels					1322:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	8	32	theme	LMWH-conjugated	1295:1309	arg1	systems					1370:1376	promising drug release systems	1347:1376	promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment	1347:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	7	33	attach	released	1146:1153	arg2	LMWH					1141:1144	LMWH	1141:1144	LMWH released from gel conjugate	1141:1172	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	7	33	attach	released	1146:1153	arg1	conjugate					1164:1172	gel conjugate	1160:1172	gel conjugate	1160:1172	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	2	34	theme	transdermal	316:326	arg1	delivery					328:335	an enhanced transdermal delivery	304:335	an enhanced transdermal delivery of low molecular weight heparin (LMWH)	304:374	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	0	35	theme	LMWH-conjugated	14:28	arg1	hydrogels					41:49	LMWH-conjugated core/shell hydrogels	14:49	LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery	14:99	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.					
30708013	2	36	theme	enhanced	307:314	arg1	delivery					328:335	an enhanced transdermal delivery	304:335	an enhanced transdermal delivery of low molecular weight heparin (LMWH)	304:374	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	5	37	theme	in	820:821	arg1	studies					828:834	In vitro and in vivo studies	807:834	In vitro and in vivo studies	807:834	In vitro and in vivo studies showed that the LMWH and PCL release was significantly affected by polymer molecular weight and block composition.					
30708013	8	38	theme	promising	1347:1355	arg1	systems					1370:1376	promising drug release systems	1347:1376	promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment	1347:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	8	38	theme	promising	1347:1355	arg1	hydrogels					1322:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	5	39	theme	molecular	911:919	arg1	weight					921:926	polymer molecular weight	903:926	polymer molecular weight	903:926	In vitro and in vivo studies showed that the LMWH and PCL release was significantly affected by polymer molecular weight and block composition.					
30708013	8	40	theme	side	1456:1459	arg1	effects					1461:1467	side effects	1456:1467	side effects	1456:1467	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	2	41	theme	cationic	413:420	arg1	enhancer					452:459	a novel permeation enhancer	433:459	a novel permeation enhancer	433:459	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	2	41	theme	cationic	413:420	arg1	carrier					422:428	cationic carrier	413:428	cationic carrier	413:428	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	0	42	theme	hydrogels	41:49	arg1	Production					0:9	Production	0:9	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.	0:133	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.					
30708013	6	43	theme	Skin	951:954	arg1	tests					968:972	Skin penetration tests	951:972	Skin penetration tests	951:972	Skin penetration tests have indicated that larger amounts of LMWH were absorbed from LMWH-gel conjugate through SC, than aqueous formula.					
30708013	7	44	theme	LMWH	1141:1144	arg1	transition					1127:1136	the plasma transition	1116:1136	the plasma transition of LMWH released from gel conjugate	1116:1172	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	7	44	theme	LMWH	1141:1144	arg1	lower					1178:1182	lower	1178:1182	lower	1178:1182	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	8	45	theme	core/shell	1311:1320	arg1	hydrogels					1322:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	PCL-loaded LMWH-conjugated core/shell hydrogels	1284:1330	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	8	45	theme	core/shell	1311:1320	arg1	systems					1370:1376	promising drug release systems	1347:1376	promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment	1347:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	2	46	theme	novel	435:439	arg1	enhancer					452:459	a novel permeation enhancer	433:459	a novel permeation enhancer	433:459	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	2	46	theme	novel	435:439	arg1	carrier					422:428	cationic carrier	413:428	cationic carrier	413:428	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	1	47	theme	promising	235:243	arg1	applications					143:154	Topical applications	135:154	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy	135:227	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy are a promising alternative strategy.					
30708013	1	47	theme	promising	235:243	arg1	strategy					257:264	a promising alternative strategy	233:264	a promising alternative strategy	233:264	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy are a promising alternative strategy.					
30708013	7	48	theme	plasma	1120:1125	arg1	transition					1127:1136	the plasma transition	1116:1136	the plasma transition of LMWH released from gel conjugate	1116:1172	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	7	48	theme	plasma	1120:1125	arg1	lower					1178:1182	lower	1178:1182	lower	1178:1182	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	6	49	theme	LMWH-gel	1036:1043	arg1	conjugate					1045:1053	LMWH-gel conjugate	1036:1053	LMWH-gel conjugate	1036:1053	Skin penetration tests have indicated that larger amounts of LMWH were absorbed from LMWH-gel conjugate through SC, than aqueous formula.					
30708013	2	50	theme	heparin	361:367	arg1	delivery					328:335	an enhanced transdermal delivery	304:335	an enhanced transdermal delivery of low molecular weight heparin (LMWH)	304:374	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	5	51	theme	In	807:808	arg1	studies					828:834	In vitro and in vivo studies	807:834	In vitro and in vivo studies	807:834	In vitro and in vivo studies showed that the LMWH and PCL release was significantly affected by polymer molecular weight and block composition.					
30708013	8	52	theme	cancer	1483:1488	arg1	treatment					1490:1498	a combined cancer treatment	1472:1498	a combined cancer treatment	1472:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	3	53	theme	high	606:609	arg1	ability					611:617	their high ability to bind growth factors	600:640	their high ability to bind growth factors	600:640	Recent studies have shown that heparin-conjugated biomaterials can be effective in inhibiting tumor growth during cancer treatment due to their high ability to bind growth factors.					
30708013	2	54	theme	weight	354:359	arg1	LMWH					370:373	LMWH	370:373	LMWH	370:373	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	2	54	theme	weight	354:359	arg1	heparin					361:367	low molecular weight heparin	340:367	low molecular weight heparin (LMWH)	340:374	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	3	55	theme	growth	627:632	arg1	factors					634:640	growth factors	627:640	growth factors	627:640	Recent studies have shown that heparin-conjugated biomaterials can be effective in inhibiting tumor growth during cancer treatment due to their high ability to bind growth factors.					
30708013	8	56	theme	combined	1474:1481	arg1	treatment					1490:1498	a combined cancer treatment	1472:1498	a combined cancer treatment	1472:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	2	57	theme	study	283:287	arg1	aim					271:273	The aim	267:273	The aim of this study	267:287	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	1	58	theme	Topical	135:141	arg1	applications					143:154	Topical applications	135:154	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy	135:227	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy are a promising alternative strategy.					
30708013	1	58	theme	Topical	135:141	arg1	strategy					257:264	a promising alternative strategy	233:264	a promising alternative strategy	233:264	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy are a promising alternative strategy.					
30708013	3	59	theme	cancer	576:581	arg1	treatment					583:591	cancer treatment	576:591	cancer treatment due to their high ability to bind growth factors	576:640	Recent studies have shown that heparin-conjugated biomaterials can be effective in inhibiting tumor growth during cancer treatment due to their high ability to bind growth factors.					
30708013	1	60	theme	alternative	245:255	arg1	applications					143:154	Topical applications	135:154	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy	135:227	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy are a promising alternative strategy.					
30708013	1	60	theme	alternative	245:255	arg1	strategy					257:264	a promising alternative strategy	233:264	a promising alternative strategy	233:264	Topical applications that reduce systemic toxic effects while increasing therapeutic efficacy are a promising alternative strategy.					
30708013	4	61	theme	cancer	779:784	arg1	treatment					786:794	a combined cancer treatment	768:794	a combined cancer treatment with LMWH	768:804	Paclitaxel (PCL) was co-encapsulated into the same cationic carrier for the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH.					
30708013	5	62	theme	polymer	903:909	arg1	weight					921:926	polymer molecular weight	903:926	polymer molecular weight	903:926	In vitro and in vivo studies showed that the LMWH and PCL release was significantly affected by polymer molecular weight and block composition.					
30708013	4	63	theme	improving	730:738	arg1	purpose					719:725	the purpose	715:725	the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH	715:804	Paclitaxel (PCL) was co-encapsulated into the same cationic carrier for the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH.					
30708013	2	64	theme	low	340:342	arg1	LMWH					370:373	LMWH	370:373	LMWH	370:373	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	2	64	theme	low	340:342	arg1	heparin					361:367	low molecular weight heparin	340:367	low molecular weight heparin (LMWH)	340:374	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	2	65	theme	molecular	344:352	arg1	LMWH					370:373	LMWH	370:373	LMWH	370:373	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	2	65	theme	molecular	344:352	arg1	heparin					361:367	low molecular weight heparin	340:367	low molecular weight heparin (LMWH)	340:374	The aim of this study was to provide an enhanced transdermal delivery of low molecular weight heparin (LMWH) through the stratum corneum by using cationic carrier as a novel permeation enhancer.					
30708013	3	66	theme	Recent	462:467	arg1	studies					469:475	Recent studies	462:475	Recent studies	462:475	Recent studies have shown that heparin-conjugated biomaterials can be effective in inhibiting tumor growth during cancer treatment due to their high ability to bind growth factors.					
30708013	4	67	theme	cationic	694:701	arg1	carrier					703:709	the same cationic carrier	685:709	the same cationic carrier for the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH	685:804	Paclitaxel (PCL) was co-encapsulated into the same cationic carrier for the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH.					
30708013	8	68	theme	transdermal	1382:1392	arg1	applications					1394:1405	transdermal applications	1382:1405	transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment	1382:1498	It is recommended that PCL-loaded LMWH-conjugated core/shell hydrogels can be used as promising drug release systems for transdermal applications that can improve therapeutic efficacy and reduce side effects in a combined cancer treatment.					
30708013	0	69	theme	transdermal	80:90	arg1	delivery					92:99	transdermal delivery	80:99	transdermal delivery	80:99	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.					
30708013	0	70	dep	Production	0:9	arg1	assessment					123:132	In vitro and in vivo assessment	102:132	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.	0:133	Production of LMWH-conjugated core/shell hydrogels encapsulating paclitaxel for transdermal delivery: In vitro and in vivo assessment.					
30708013	4	71	theme	same	689:692	arg1	carrier					703:709	the same cationic carrier	685:709	the same cationic carrier for the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH	685:804	Paclitaxel (PCL) was co-encapsulated into the same cationic carrier for the purpose of improving of therapeutic efficacy for a combined cancer treatment with LMWH.					
30708013	7	72	theme	LMWH	1224:1227	arg1	concentration					1207:1219	the plasma concentration	1196:1219	the plasma concentration of LMWH released from aqueous solution	1196:1258	However, it was found that the plasma transition of LMWH released from gel conjugate was lower compared to the plasma concentration of LMWH released from aqueous solution.					
30708013	6	73	theme	LMWH	1012:1015	arg1	amounts					1001:1007	larger amounts	994:1007	larger amounts of LMWH	994:1015	Skin penetration tests have indicated that larger amounts of LMWH were absorbed from LMWH-gel conjugate through SC, than aqueous formula.					
30708013	6	73	theme	LMWH	1012:1015	arg1	LMWH					1012:1015	LMWH	1012:1015	LMWH	1012:1015	Skin penetration tests have indicated that larger amounts of LMWH were absorbed from LMWH-gel conjugate through SC, than aqueous formula.					
31653107	0	0	theme	Challenge	69:77	arg1	Models					49:54	Rodent Models	42:54	Rodent Models of Lactation Challenge	42:77	Impact of Fenugreek on Milk Production in Rodent Models of Lactation Challenge.					
31653107	4	1	theme	20	612:613	arg1	%					614:614	%	614:614	%	614:614	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	9	2	contain	had	1215:1217	arg2	effect					1222:1227	no effect	1219:1227	no effect	1219:1227	Fenugreek had no effect in the maternal protein restriction challenge.					
31653107	9	2	contain	had	1215:1217	arg1	Fenugreek					1205:1213	Fenugreek	1205:1213	Fenugreek	1205:1213	Fenugreek had no effect in the maternal protein restriction challenge.					
31653107	4	3	theme	protein	616:622	arg1	diet					624:627	a 20% protein diet	610:627	a 20% protein diet	610:627	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	1	4	theme	milk	222:225	arg1	production					227:236	perceived insufficient milk production	199:236	perceived insufficient milk production	199:236	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
31653107	0	5	theme	Lactation	59:67	arg1	Challenge					69:77	Lactation Challenge	59:77	Lactation Challenge	59:77	Impact of Fenugreek on Milk Production in Rodent Models of Lactation Challenge.					
31653107	10	6	theme	fenugreek	1323:1331	arg1	effect					1313:1318	a galactologue effect	1298:1318	a galactologue effect of fenugreek when mothers have no physiological difficulties in producing milk	1298:1397	These results suggest a galactologue effect of fenugreek when mothers have no physiological difficulties in producing milk.					
31653107	5	7	theme	control	783:789	arg1	dams					791:794	control dams	783:794	control dams fed 20% protein diet with eight pups' litters	783:840	day-1 dietary supplementation of fenugreek, compared to control dams fed 20% protein diet with eight pups' litters.					
31653107	4	8	theme	%	614:614	arg1	diet					624:627	a 20% protein diet	610:627	a 20% protein diet	610:627	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	3	9	from	effects	486:492	arg1	offspring					505:513	offspring	505:513	offspring	505:513	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	3	9	from	effects	486:492	arg1	dam					497:499	dam	497:499	dam	497:499	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	10	10	theme	galactologue	1300:1311	arg1	effect					1313:1318	a galactologue effect	1298:1318	a galactologue effect of fenugreek when mothers have no physiological difficulties in producing milk	1298:1397	These results suggest a galactologue effect of fenugreek when mothers have no physiological difficulties in producing milk.					
31653107	8	11	theme	Fenugreek	1027:1035	arg1	production					1052:1061	Fenugreek increased milk production	1027:1061	Fenugreek increased milk production by 16% in the litter size	1027:1087	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	0	12	from	Impact	0:5	arg1	Production					28:37	Milk Production	23:37	Milk Production in Rodent Models of Lactation Challenge	23:77	Impact of Fenugreek on Milk Production in Rodent Models of Lactation Challenge.					
31653107	10	13	from	difficulties	1368:1379	arg1	milk					1394:1397	producing milk	1384:1397	producing milk	1384:1397	These results suggest a galactologue effect of fenugreek when mothers have no physiological difficulties in producing milk.					
31653107	3	14	theme	effects	486:492	arg1	lack					470:473	the lack	466:473	the lack of adverse effects on dam and offspring metabolism	466:524	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	4	15	theme	litter	575:580	arg1	size					582:585	increased litter size	565:585	increased litter size to 12 pups in dams fed a 20% protein diet	565:627	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	4	16	theme	protein	664:670	arg1	diet					672:675	an 8% protein diet	658:675	an 8% protein diet	658:675	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	8	17	theme	dam-litter	1182:1191	arg1	metabolism					1193:1202	dam-litter metabolism	1182:1202	dam-litter metabolism	1182:1202	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	1	18	used	used	122:125	arg2	galactologue					130:141	galactologue	130:141	galactologue	130:141	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
31653107	1	18	used	used	122:125	arg2	remedy					100:105	a herbal remedy	91:105	a herbal remedy	91:105	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
31653107	4	19	theme	lactation	531:539	arg1	challenges					541:550	Two lactation challenges	527:550	Two lactation challenges	527:550	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	8	20	theme	milk	1047:1050	arg1	production					1052:1061	Fenugreek increased milk production	1027:1061	Fenugreek increased milk production by 16% in the litter size	1027:1087	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	9	21	theme	protein	1245:1251	arg1	challenge					1265:1273	the maternal protein restriction challenge	1232:1273	the maternal protein restriction challenge	1232:1273	Fenugreek had no effect in the maternal protein restriction challenge.					
31653107	7	22	theme	glucose	953:959	arg1	metabolism					961:970	glucose metabolism	953:970	glucose metabolism	953:970	Lipid and glucose metabolism parameters were assessed in dam and offspring plasmas.					
31653107	3	23	from	lack	470:473	arg1	offspring					505:513	offspring	505:513	offspring	505:513	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	3	23	from	lack	470:473	arg1	dam					497:499	dam	497:499	dam	497:499	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	2	24	theme	few	248:250	arg1	studies					252:258	few studies	248:258	few studies	248:258	However, few studies highlight the efficacy of fenugreek in enhancing milk production.					
31653107	5	25	theme	fenugreek	760:768	arg1	supplementation					741:755	day-1 dietary supplementation	727:755	day-1 dietary supplementation	727:755	day-1 dietary supplementation of fenugreek, compared to control dams fed 20% protein diet with eight pups' litters.					
31653107	8	26	theme	increased	1037:1045	arg1	production					1052:1061	Fenugreek increased milk production	1027:1061	Fenugreek increased milk production by 16% in the litter size	1027:1087	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	0	27	from	Production	28:37	arg1	Models					49:54	Rodent Models	42:54	Rodent Models of Lactation Challenge	42:77	Impact of Fenugreek on Milk Production in Rodent Models of Lactation Challenge.					
31653107	7	28	theme	metabolism	961:970	arg1	parameters					972:981	Lipid and glucose metabolism parameters	943:981	Lipid and glucose metabolism parameters	943:981	Lipid and glucose metabolism parameters were assessed in dam and offspring plasmas.					
31653107	3	29	theme	adverse	478:484	arg1	effects					486:492	adverse effects	478:492	adverse effects on dam and offspring metabolism	478:524	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	3	30	theme	rodent	408:413	arg1	models					415:420	rodent models	408:420	rodent models of lactation challenge	408:443	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	8	31	from	increase	1129:1136	arg1	growth					1145:1150	pup growth	1141:1150	pup growth	1141:1150	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	7	32	theme	Lipid	943:947	arg1	parameters					972:981	Lipid and glucose metabolism parameters	943:981	Lipid and glucose metabolism parameters	943:981	Lipid and glucose metabolism parameters were assessed in dam and offspring plasmas.					
31653107	8	33	from	effect	1172:1177	arg1	metabolism					1193:1202	dam-litter metabolism	1182:1202	dam-litter metabolism	1182:1202	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	2	34	theme	fenugreek	286:294	arg1	efficacy					274:281	the efficacy	270:281	the efficacy of fenugreek in enhancing milk production	270:323	However, few studies highlight the efficacy of fenugreek in enhancing milk production.					
31653107	4	35	from	pups	593:596	arg1	dams					601:604	dams	601:604	dams fed a 20% protein diet	601:627	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	3	36	theme	milk	394:397	arg1	yield					399:403	milk yield	394:403	milk yield	394:403	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	6	37	theme	Milk	843:846	arg1	flow					848:851	Milk flow	843:851	Milk flow	843:851	Milk flow was measured by the deuterium oxide enrichment method, and milk composition was assessed.					
31653107	1	38	theme	Fenugreek	80:88	arg1	galactologue					130:141	galactologue	130:141	galactologue	130:141	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
31653107	1	38	theme	Fenugreek	80:88	arg1	remedy					100:105	a herbal remedy	91:105	a herbal remedy	91:105	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
31653107	9	39	theme	restriction	1253:1263	arg1	challenge					1265:1273	the maternal protein restriction challenge	1232:1273	the maternal protein restriction challenge	1232:1273	Fenugreek had no effect in the maternal protein restriction challenge.					
31653107	8	40	theme	pup	1141:1143	arg1	growth					1145:1150	pup growth	1141:1150	pup growth	1141:1150	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	5	41	theme	protein	804:810	arg1	diet					812:815	20% protein diet	800:815	20% protein diet	800:815	day-1 dietary supplementation of fenugreek, compared to control dams fed 20% protein diet with eight pups' litters.					
31653107	4	42	theme	%	662:662	arg1	diet					672:675	an 8% protein diet	658:675	an 8% protein diet	658:675	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	3	43	theme	lactation	425:433	arg1	challenge					435:443	lactation challenge	425:443	lactation challenge	425:443	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	3	44	theme	study	342:346	arg1	aims					330:333	The aims	326:333	The aims of our study	326:346	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	4	45	with	diet	672:675	arg1	litter					694:699	eight pups' litter	682:699	eight pups' litter	682:699	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	6	46	theme	deuterium	873:881	arg1	method					900:905	the deuterium oxide enrichment method	869:905	the deuterium oxide enrichment method	869:905	Milk flow was measured by the deuterium oxide enrichment method, and milk composition was assessed.					
31653107	4	47	theme	8	661:661	arg1	%					662:662	%	662:662	%	662:662	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	3	48	theme	challenge	435:443	arg1	models					415:420	rodent models	408:420	rodent models of lactation challenge	408:443	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	5	49	theme	20	800:801	arg1	%					802:802	%	802:802	%	802:802	day-1 dietary supplementation of fenugreek, compared to control dams fed 20% protein diet with eight pups' litters.					
31653107	5	50	theme	day-1	727:731	arg1	supplementation					741:755	day-1 dietary supplementation	727:755	day-1 dietary supplementation	727:755	day-1 dietary supplementation of fenugreek, compared to control dams fed 20% protein diet with eight pups' litters.					
31653107	1	51	theme	stop	169:172	arg1	breastfeeding					174:186	stop breastfeeding	169:186	stop breastfeeding	169:186	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
31653107	0	52	theme	Milk	23:26	arg1	Production					28:37	Milk Production	23:37	Milk Production in Rodent Models of Lactation Challenge	23:77	Impact of Fenugreek on Milk Production in Rodent Models of Lactation Challenge.					
31653107	8	53	theme	%	1127:1127	arg1	increase					1129:1136	an 11% increase	1122:1136	an 11% increase in pup growth	1122:1150	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	4	54	theme	increased	565:573	arg1	size					582:585	increased litter size	565:585	increased litter size to 12 pups in dams fed a 20% protein diet	565:627	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	5	55	theme	dietary	733:739	arg1	supplementation					741:755	day-1 dietary supplementation	727:755	day-1 dietary supplementation	727:755	day-1 dietary supplementation of fenugreek, compared to control dams fed 20% protein diet with eight pups' litters.					
31653107	0	56	theme	Rodent	42:47	arg1	Models					49:54	Rodent Models	42:54	Rodent Models of Lactation Challenge	42:77	Impact of Fenugreek on Milk Production in Rodent Models of Lactation Challenge.					
31653107	1	57	theme	herbal	93:98	arg1	galactologue					130:141	galactologue	130:141	galactologue	130:141	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
31653107	1	57	theme	herbal	93:98	arg1	remedy					100:105	a herbal remedy	91:105	a herbal remedy	91:105	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
31653107	8	58	theme	litter	1077:1082	arg1	size					1084:1087	the litter size	1073:1087	the litter size	1073:1087	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	9	59	theme	maternal	1236:1243	arg1	challenge					1265:1273	the maternal protein restriction challenge	1232:1273	the maternal protein restriction challenge	1232:1273	Fenugreek had no effect in the maternal protein restriction challenge.					
31653107	10	60	contain	have	1346:1349	arg2	difficulties					1368:1379	no physiological difficulties	1351:1379	no physiological difficulties in producing milk	1351:1397	These results suggest a galactologue effect of fenugreek when mothers have no physiological difficulties in producing milk.					
31653107	10	60	contain	have	1346:1349	arg1	mothers					1338:1344	mothers	1338:1344	mothers	1338:1344	These results suggest a galactologue effect of fenugreek when mothers have no physiological difficulties in producing milk.					
31653107	2	61	theme	milk	309:312	arg1	production					314:323	milk production	309:323	milk production	309:323	However, few studies highlight the efficacy of fenugreek in enhancing milk production.					
31653107	3	62	from	dam	497:499	arg1	lack					470:473	the lack	466:473	the lack of adverse effects on dam and offspring metabolism	466:524	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	10	63	theme	producing	1384:1392	arg1	milk					1394:1397	producing milk	1384:1397	producing milk	1384:1397	These results suggest a galactologue effect of fenugreek when mothers have no physiological difficulties in producing milk.					
31653107	5	64	theme	%	802:802	arg1	diet					812:815	20% protein diet	800:815	20% protein diet	800:815	day-1 dietary supplementation of fenugreek, compared to control dams fed 20% protein diet with eight pups' litters.					
31653107	6	65	theme	enrichment	889:898	arg1	method					900:905	the deuterium oxide enrichment method	869:905	the deuterium oxide enrichment method	869:905	Milk flow was measured by the deuterium oxide enrichment method, and milk composition was assessed.					
31653107	4	66	dep	tested	557:562	arg1	size					582:585	increased litter size	565:585	increased litter size to 12 pups in dams fed a 20% protein diet	565:627	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	4	66	dep	tested	557:562	arg1	restriction					643:653	perinatal restriction	633:653	perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1	633:725	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	8	67	theme	deleterious	1160:1170	arg1	effect					1172:1177	deleterious effect	1160:1177	deleterious effect on dam-litter metabolism	1160:1202	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	4	68	theme	perinatal	633:641	arg1	restriction					643:653	perinatal restriction	633:653	perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1	633:725	Two lactation challenges were tested: increased litter size to 12 pups in dams fed a 20% protein diet and perinatal restriction to an 8% protein diet with eight pups' litter, with or without 1 g.kg-1.					
31653107	3	69	from	offspring	505:513	arg1	lack					470:473	the lack	466:473	the lack of adverse effects on dam and offspring metabolism	466:524	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	6	70	theme	oxide	883:887	arg1	method					900:905	the deuterium oxide enrichment method	869:905	the deuterium oxide enrichment method	869:905	Milk flow was measured by the deuterium oxide enrichment method, and milk composition was assessed.					
31653107	10	71	theme	physiological	1354:1366	arg1	difficulties					1368:1379	no physiological difficulties	1351:1379	no physiological difficulties in producing milk	1351:1397	These results suggest a galactologue effect of fenugreek when mothers have no physiological difficulties in producing milk.					
31653107	6	72	theme	milk	912:915	arg1	composition					917:927	milk composition	912:927	milk composition	912:927	Milk flow was measured by the deuterium oxide enrichment method, and milk composition was assessed.					
31653107	3	73	dep	dam	497:499	arg1	metabolism					515:524	metabolism	515:524	metabolism	515:524	The aims of our study were to determine whether fenugreek increased milk yield in rodent models of lactation challenge and if so, to verify the lack of adverse effects on dam and offspring metabolism.					
31653107	1	74	theme	perceived	199:207	arg1	production					227:236	perceived insufficient milk production	199:236	perceived insufficient milk production	199:236	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
31653107	8	75	from	%	1068:1068	arg1	size					1084:1087	the litter size	1073:1087	the litter size	1073:1087	Fenugreek increased milk production by 16% in the litter size increase challenge, resulting in an 11% increase in pup growth without deleterious effect on dam-litter metabolism.					
31653107	0	76	theme	Fenugreek	10:18	arg1	Impact					0:5	Impact	0:5	Impact of Fenugreek on Milk Production in Rodent Models of Lactation Challenge.	0:78	Impact of Fenugreek on Milk Production in Rodent Models of Lactation Challenge.					
31653107	5	77	dep	pups	828:831	arg1	litters					834:840	litters	834:840	eight pups' litters	822:840	day-1 dietary supplementation of fenugreek, compared to control dams fed 20% protein diet with eight pups' litters.					
31653107	7	78	dep	dam	1000:1002	arg1	plasmas					1018:1024	plasmas	1018:1024	plasmas	1018:1024	Lipid and glucose metabolism parameters were assessed in dam and offspring plasmas.					
31653107	1	79	theme	insufficient	209:220	arg1	production					227:236	perceived insufficient milk production	199:236	perceived insufficient milk production	199:236	Fenugreek, a herbal remedy, has long been used as galactologue to help mothers likely to stop breastfeeding because of perceived insufficient milk production.					
29944125	2	0	theme	adsorbent	557:565	arg1	dosage					567:572	adsorbent dosage	557:572	adsorbent dosage	557:572	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	3	1	theme	determining	925:935	arg1	concentration					903:915	the initial concentration	891:915	the initial concentration	891:915	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	3	1	theme	determining	925:935	arg1	factor					937:942	the determining factor	921:942	the determining factor for optimum efficiency for the dye RR136	921:983	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	4	2	theme	respective	992:1001	arg1	condition					1011:1019	respective optimal condition	992:1019	respective optimal condition	992:1019	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	2	3	theme	simultaneous	606:617	arg1	maximization					619:630	simultaneous maximization	606:630	simultaneous maximization of removal percentage and adsorption capacity	606:676	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	3	4	theme	RB5	838:840	arg1	removal					827:833	removal	827:833	removal of RB5, RY145 and RB222	827:857	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	3	5	theme	effective	804:812	arg1	pH					879:880	the dye solution pH	862:880	the dye solution pH	862:880	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	3	5	theme	effective	804:812	arg1	parameter					814:822	the most effective parameter	795:822	the most effective parameter in removal of RB5, RY145 and RB222	795:857	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	4	6	dep	percentages	1034:1044	arg1	the					1022:1024	the	1022:1024	the	1022:1024	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	6	7	theme	adsorption	1369:1378	arg1	kinetics					1380:1387	the adsorption kinetics	1365:1387	the adsorption kinetics	1365:1387	The adsorption behaviors showed that the adsorption kinetics and isotherms were in best agreement with Avrami fractionary order model and the Toth isotherm, respectively.					
29944125	2	8	theme	adsorption	477:486	arg1	conditions					488:497	the optimal adsorption conditions	465:497	the optimal adsorption conditions	465:497	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	8	theme	adsorption	477:486	arg1	concentration					522:534	initial concentration	514:534	initial concentration of dye	514:541	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	8	theme	adsorption	477:486	arg1	dosage					567:572	adsorbent dosage	557:572	adsorbent dosage	557:572	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	8	theme	adsorption	477:486	arg1	time					586:589	contact time	578:589	contact time	578:589	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	8	theme	adsorption	477:486	arg1	pH					510:511	pH	510:511	pH	510:511	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	8	theme	adsorption	477:486	arg1	temperature					544:554	temperature	544:554	temperature	544:554	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	1	9	theme	Reactive	332:339	arg1	Yellow					341:346	Reactive Yellow 145 (RY145)	332:358	Reactive Yellow 145 (RY145)	332:358	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	3	10	theme	optimum	948:954	arg1	efficiency					956:965	optimum efficiency	948:965	optimum efficiency	948:965	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	4	11	theme	dyes	1087:1090	arg1	percentages					1034:1044	removal percentages	1026:1044	removal percentages	1026:1044	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	4	11	theme	dyes	1087:1090	arg1	capacity					1061:1068	adsorption capacity	1050:1068	adsorption capacity	1050:1068	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	2	12	theme	optimal	469:475	arg1	conditions					488:497	the optimal adsorption conditions	465:497	the optimal adsorption conditions	465:497	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	12	theme	optimal	469:475	arg1	concentration					522:534	initial concentration	514:534	initial concentration of dye	514:541	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	12	theme	optimal	469:475	arg1	dosage					567:572	adsorbent dosage	557:572	adsorbent dosage	557:572	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	12	theme	optimal	469:475	arg1	time					586:589	contact time	578:589	contact time	578:589	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	12	theme	optimal	469:475	arg1	pH					510:511	pH	510:511	pH	510:511	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	12	theme	optimal	469:475	arg1	temperature					544:554	temperature	544:554	temperature	544:554	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	4	13	dep	found	1115:1119	arg1	>					1165:1165	>	1165:1165	>	1165:1165	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	5	14	theme	maximum	1178:1184	arg1	percentages					1194:1204	The maximum removal percentages	1174:1204	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g	1174:1277	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	3	15	theme	dye	866:868	arg1	pH					879:880	the dye solution pH	862:880	the dye solution pH	862:880	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	3	15	theme	dye	866:868	arg1	parameter					814:822	the most effective parameter	795:822	the most effective parameter in removal of RB5, RY145 and RB222	795:857	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	0	16	theme	isotherm	120:127	arg1	optimization					97:108	composite: multi-response optimization	71:108	composite: multi-response optimization	71:108	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	0	16	theme	isotherm	120:127	arg1	study					147:151	kinetic, isotherm and thermodynamic study	111:151	kinetic, isotherm and thermodynamic study	111:151	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	2	17	theme	contact	578:584	arg1	time					586:589	contact time	578:589	contact time	578:589	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	18	theme	percentage	643:652	arg1	maximization					619:630	simultaneous maximization	606:630	simultaneous maximization of removal percentage and adsorption capacity	606:676	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	3	19	theme	RB222	853:857	arg1	removal					827:833	removal	827:833	removal of RB5, RY145 and RB222	827:857	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	4	20	located	found	1115:1119	arg2	percentages					1034:1044	removal percentages	1026:1044	removal percentages	1026:1044	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	4	20	located	found	1115:1119	arg1	order					1138:1142	the following order	1124:1142	the following order	1124:1142	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	4	20	located	found	1115:1119	arg2	capacity					1061:1068	adsorption capacity	1050:1068	adsorption capacity	1050:1068	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	5	21	theme	removal	1186:1192	arg1	percentages					1194:1204	The maximum removal percentages	1174:1204	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g	1174:1277	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	3	22	theme	percentage	683:692	arg1	contribution					694:705	The percentage contribution	679:705	The percentage contribution of each adsorption condition	679:734	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	2	23	dep	method	399:404	arg1	analysis					431:438	attached grey relational analysis	406:438	Taguchi method attached grey relational analysis	391:438	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	24	theme	removal	635:641	arg1	percentage					643:652	removal percentage	635:652	removal percentage	635:652	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	3	25	from	parameter	814:822	arg1	removal					827:833	removal	827:833	removal of RB5, RY145 and RB222	827:857	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	6	26	theme	fractionary	1438:1448	arg1	model					1456:1460	Avrami fractionary order model	1431:1460	Avrami fractionary order model	1431:1460	The adsorption behaviors showed that the adsorption kinetics and isotherms were in best agreement with Avrami fractionary order model and the Toth isotherm, respectively.					
29944125	2	27	theme	Taguchi	391:397	arg1	method					399:404	Taguchi method attached grey relational analysis	391:438	Taguchi method attached grey relational analysis	391:438	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	3	28	theme	condition	726:734	arg1	contribution					694:705	The percentage contribution	679:705	The percentage contribution of each adsorption condition	679:734	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	3	29	theme	dye	975:977	arg1	RR136					979:983	the dye RR136	971:983	the dye RR136	971:983	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	5	30	theme	%	1222:1222	arg1	percentages					1194:1204	The maximum removal percentages	1174:1204	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g	1174:1277	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	0	31	theme	dyes	23:26	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of reactive dyes onto chitosan/montmorillonite	0:56	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	4	32	theme	adsorption	1050:1059	arg1	capacity					1061:1068	adsorption capacity	1050:1068	adsorption capacity	1050:1068	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	0	33	theme	reactive	14:21	arg1	dyes					23:26	reactive dyes	14:26	reactive dyes	14:26	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	0	34	theme	thermodynamic	133:145	arg1	optimization					97:108	composite: multi-response optimization	71:108	composite: multi-response optimization	71:108	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	0	34	theme	thermodynamic	133:145	arg1	study					147:151	kinetic, isotherm and thermodynamic study	111:151	kinetic, isotherm and thermodynamic study	111:151	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	3	35	theme	solution	870:877	arg1	pH					879:880	the dye solution pH	862:880	the dye solution pH	862:880	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	3	35	theme	solution	870:877	arg1	parameter					814:822	the most effective parameter	795:822	the most effective parameter in removal of RB5, RY145 and RB222	795:857	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	6	36	theme	Avrami	1431:1436	arg1	model					1456:1460	Avrami fractionary order model	1431:1460	Avrami fractionary order model	1431:1460	The adsorption behaviors showed that the adsorption kinetics and isotherms were in best agreement with Avrami fractionary order model and the Toth isotherm, respectively.					
29944125	3	37	theme	adsorption	715:724	arg1	condition					726:734	each adsorption condition	710:734	each adsorption condition	710:734	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	1	38	dep	dyes	271:274	arg1	136					319:321	136	319:321	136	319:321	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	38	dep	dyes	271:274	arg1	Yellow					341:346	Reactive Yellow 145 (RY145)	332:358	Reactive Yellow 145 (RY145)	332:358	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	38	dep	dyes	271:274	arg1	5					297:297	5	297:297	5	297:297	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	38	dep	dyes	271:274	arg1	RB5					300:302	RB5	300:302	RB5	300:302	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	38	dep	dyes	271:274	arg1	RR136					324:328	RR136	324:328	RR136	324:328	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	38	dep	dyes	271:274	arg1	RB222					383:387	RB222	383:387	RB222	383:387	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	6	39	theme	best	1411:1414	arg1	agreement					1416:1424	best agreement	1411:1424	best agreement with Avrami fractionary order model and the Toth isotherm	1411:1482	The adsorption behaviors showed that the adsorption kinetics and isotherms were in best agreement with Avrami fractionary order model and the Toth isotherm, respectively.					
29944125	2	40	theme	relational	420:429	arg1	analysis					431:438	attached grey relational analysis	406:438	Taguchi method attached grey relational analysis	391:438	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	6	41	theme	Toth	1470:1473	arg1	isotherm					1475:1482	the Toth isotherm	1466:1482	the Toth isotherm	1466:1482	The adsorption behaviors showed that the adsorption kinetics and isotherms were in best agreement with Avrami fractionary order model and the Toth isotherm, respectively.					
29944125	4	42	theme	following	1128:1136	arg1	order					1138:1142	the following order	1124:1142	the following order	1124:1142	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	3	43	theme	variance	770:777	arg1	analysis					758:765	the analysis	754:765	the analysis of variance	754:777	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	0	44	theme	composite	71:79	arg1	optimization					97:108	composite: multi-response optimization	71:108	composite: multi-response optimization	71:108	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	0	44	theme	composite	71:79	arg1	study					147:151	kinetic, isotherm and thermodynamic study	111:151	kinetic, isotherm and thermodynamic study	111:151	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	4	45	theme	reactive	1078:1085	arg1	dyes					1087:1090	four reactive dyes	1073:1090	four reactive dyes	1073:1090	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	2	46	theme	dye	539:541	arg1	pH					510:511	pH	510:511	pH	510:511	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	46	theme	dye	539:541	arg1	concentration					522:534	initial concentration	514:534	initial concentration of dye	514:541	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	46	theme	dye	539:541	arg1	temperature					544:554	temperature	544:554	temperature	544:554	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	46	theme	dye	539:541	arg1	dosage					567:572	adsorbent dosage	557:572	adsorbent dosage	557:572	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	46	theme	dye	539:541	arg1	time					586:589	contact time	578:589	contact time	578:589	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	47	theme	capacity	669:676	arg1	maximization					619:630	simultaneous maximization	606:630	simultaneous maximization of removal percentage and adsorption capacity	606:676	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	2	48	theme	attached	406:413	arg1	analysis					431:438	attached grey relational analysis	406:438	Taguchi method attached grey relational analysis	391:438	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	5	49	theme	adsorption	1233:1242	arg1	capacity					1244:1251	the adsorption capacity	1229:1251	the adsorption capacity of 315.20 and 123.75 mg/g	1229:1277	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	2	50	theme	adsorption	658:667	arg1	capacity					669:676	adsorption capacity	658:676	adsorption capacity	658:676	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	4	51	theme	removal	1026:1032	arg1	percentages					1034:1044	removal percentages	1026:1044	removal percentages	1026:1044	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	6	52	with	agreement	1416:1424	arg1	isotherm					1475:1482	the Toth isotherm	1466:1482	the Toth isotherm	1466:1482	The adsorption behaviors showed that the adsorption kinetics and isotherms were in best agreement with Avrami fractionary order model and the Toth isotherm, respectively.					
29944125	6	52	with	agreement	1416:1424	arg1	model					1456:1460	Avrami fractionary order model	1431:1460	Avrami fractionary order model	1431:1460	The adsorption behaviors showed that the adsorption kinetics and isotherms were in best agreement with Avrami fractionary order model and the Toth isotherm, respectively.					
29944125	3	53	theme	initial	895:901	arg1	concentration					903:915	the initial concentration	891:915	the initial concentration	891:915	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	3	53	theme	initial	895:901	arg1	factor					937:942	the determining factor	921:942	the determining factor for optimum efficiency for the dye RR136	921:983	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	5	54	theme	78.8	1209:1212	arg1	mg/g					1274:1277	315.20 and 123.75 mg/g	1256:1277	315.20 and 123.75 mg/g	1256:1277	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	5	54	theme	78.8	1209:1212	arg1	%					1222:1222	78.8 and 49.5%	1209:1222	%	1222:1222	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	2	55	theme	initial	514:520	arg1	concentration					522:534	initial concentration	514:534	initial concentration of dye	514:541	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	1	56	used	used	216:219	arg2	CTS/MMT					203:209	Chitosan/montmorillonite intercalated composite (CTS/MMT)	154:210	Chitosan/montmorillonite intercalated composite (CTS/MMT)	154:210	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	56	used	used	216:219	arg2	adsorbent					237:245	adsorbent	237:245	adsorbent	237:245	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	57	theme	Chitosan/montmorillonite	154:177	arg1	adsorbent					237:245	adsorbent	237:245	adsorbent	237:245	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	57	theme	Chitosan/montmorillonite	154:177	arg1	CTS/MMT					203:209	Chitosan/montmorillonite intercalated composite (CTS/MMT)	154:210	Chitosan/montmorillonite intercalated composite (CTS/MMT)	154:210	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	6	58	theme	order	1450:1454	arg1	model					1456:1460	Avrami fractionary order model	1431:1460	Avrami fractionary order model	1431:1460	The adsorption behaviors showed that the adsorption kinetics and isotherms were in best agreement with Avrami fractionary order model and the Toth isotherm, respectively.					
29944125	0	59	theme	multi-response	82:95	arg1	optimization					97:108	composite: multi-response optimization	71:108	composite: multi-response optimization	71:108	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	0	59	theme	multi-response	82:95	arg1	study					147:151	kinetic, isotherm and thermodynamic study	111:151	kinetic, isotherm and thermodynamic study	111:151	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	6	60	theme	adsorption	1332:1341	arg1	behaviors					1343:1351	The adsorption behaviors	1328:1351	The adsorption behaviors	1328:1351	The adsorption behaviors showed that the adsorption kinetics and isotherms were in best agreement with Avrami fractionary order model and the Toth isotherm, respectively.					
29944125	4	61	theme	optimal	1003:1009	arg1	condition					1011:1019	respective optimal condition	992:1019	respective optimal condition	992:1019	Under respective optimal condition, the removal percentages and adsorption capacity of four reactive dyes onto CTS/MMT were both found in the following order: RR136 > RY145 > RB5 > RB222.					
29944125	1	62	theme	intercalated	179:190	arg1	adsorbent					237:245	adsorbent	237:245	adsorbent	237:245	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	62	theme	intercalated	179:190	arg1	CTS/MMT					203:209	Chitosan/montmorillonite intercalated composite (CTS/MMT)	154:210	Chitosan/montmorillonite intercalated composite (CTS/MMT)	154:210	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	5	63	theme	49.5	1218:1221	arg1	mg/g					1274:1277	315.20 and 123.75 mg/g	1256:1277	315.20 and 123.75 mg/g	1256:1277	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	5	63	theme	49.5	1218:1221	arg1	%					1222:1222	78.8 and 49.5%	1209:1222	%	1222:1222	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	1	64	theme	reactive	262:269	arg1	dyes					271:274	reactive dyes	262:274	reactive dyes	262:274	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	2	65	theme	grey	415:418	arg1	analysis					431:438	attached grey relational analysis	406:438	Taguchi method attached grey relational analysis	391:438	Taguchi method attached grey relational analysis was applied to determine the optimal adsorption conditions, including pH, initial concentration of dye, temperature, adsorbent dosage and contact time, for achieving simultaneous maximization of removal percentage and adsorption capacity.					
29944125	5	66	theme	capacity	1244:1251	arg1	percentages					1194:1204	The maximum removal percentages	1174:1204	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g	1174:1277	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	1	67	theme	dyes	271:274	arg1	removal					251:257	removal	251:257	removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222)	251:388	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	5	68	theme	mg/g	1274:1277	arg1	mg/g					1274:1277	315.20 and 123.75 mg/g	1256:1277	315.20 and 123.75 mg/g	1256:1277	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	5	68	theme	mg/g	1274:1277	arg1	capacity					1244:1251	the adsorption capacity	1229:1251	the adsorption capacity of 315.20 and 123.75 mg/g	1229:1277	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	5	68	theme	mg/g	1274:1277	arg1	%					1222:1222	78.8 and 49.5%	1209:1222	%	1222:1222	The maximum removal percentages of 78.8 and 49.5%, and the adsorption capacity of 315.20 and 123.75 mg/g were obtained for RR136 and RB222, respectively.					
29944125	1	69	theme	composite	192:200	arg1	adsorbent					237:245	adsorbent	237:245	adsorbent	237:245	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	1	69	theme	composite	192:200	arg1	CTS/MMT					203:209	Chitosan/montmorillonite intercalated composite (CTS/MMT)	154:210	Chitosan/montmorillonite intercalated composite (CTS/MMT)	154:210	Chitosan/montmorillonite intercalated composite (CTS/MMT) was used as an effective adsorbent for removal of reactive dyes, i.e. Reactive Black 5 (RB5), Reactive Red 136 (RR136), Reactive Yellow 145 (RY145) and Reactive Blue 222 (RB222).					
29944125	3	70	theme	RY145	843:847	arg1	removal					827:833	removal	827:833	removal of RB5, RY145 and RB222	827:857	The percentage contribution of each adsorption condition was determined in the analysis of variance and showed that the most effective parameter in removal of RB5, RY145 and RB222 is the dye solution pH, whereas the initial concentration was the determining factor for optimum efficiency for the dye RR136.					
29944125	0	71	theme	kinetic	111:117	arg1	optimization					97:108	composite: multi-response optimization	71:108	composite: multi-response optimization	71:108	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
29944125	0	71	theme	kinetic	111:117	arg1	study					147:151	kinetic, isotherm and thermodynamic study	111:151	kinetic, isotherm and thermodynamic study	111:151	Adsorption of reactive dyes onto chitosan/montmorillonite intercalated composite: multi-response optimization, kinetic, isotherm and thermodynamic study.					
30756353	7	0	from	wastewater	1282:1291	arg1	uptake					1259:1264	uptake As	1259:1267	uptake As(V) from acid wastewater	1259:1291	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	0	from	wastewater	1282:1291	arg1	V					1269:1269	V	1269:1269	V	1269:1269	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	1	theme	Al-OH	1227:1231	arg1	group					1218:1222	the surface hydroxyl group	1197:1222	the surface hydroxyl group of Al-OH and -NH2	1197:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	1	theme	Al-OH	1227:1231	arg1	As-N					1179:1182	As-N	1179:1182	As-N	1179:1182	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	1	theme	Al-OH	1227:1231	arg1	As-O					1188:1191	As-O	1188:1191	As-O	1188:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	1	theme	Al-OH	1227:1231	arg1	Al-OH					1227:1231	Al-OH	1227:1231	Al-OH	1227:1231	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	1	theme	Al-OH	1227:1231	arg1	-NH2					1237:1240	-NH2	1237:1240	-NH2	1237:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	1	theme	Al-OH	1227:1231	arg1	bond					1171:1174	the bond	1167:1174	the bond of As-N and As-O	1167:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	1	2	theme	fly	132:134	arg1	material					207:214	pristine material	198:214	pristine material	198:214	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	1	2	theme	fly	132:134	arg1	ash					136:138	Solid waste fly ash	120:138	Solid waste fly ash with low aluminum of Yunnan Province in China	120:184	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	0	3	theme	fly	84:86	arg1	ash					88:90	fly ash	84:90	fly ash	84:90	Characterizations and mechanisms for synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study.					
30756353	7	4	theme	As	1266:1267	arg1	uptake					1259:1264	uptake As	1259:1267	uptake As(V) from acid wastewater	1259:1291	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	4	theme	As	1266:1267	arg1	V					1269:1269	V	1269:1269	V	1269:1269	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	1	5	with	ash	136:138	arg1	aluminum					149:156	low aluminum	145:156	low aluminum of Yunnan Province	145:175	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	3	6	theme	zeolite	557:563	arg1	Si-OH					530:534	Si-OH	530:534	Si-OH of the obtained Na-X zeolite and C-OH of chitosan	530:584	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	0	7	theme	As	96:97	arg1	study					113:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	Characterizations and mechanisms for synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study.					
30756353	3	8	theme	dominated	594:602	arg1	mechanism					604:612	the dominated mechanism	590:612	the dominated mechanism for coated chitosan	590:632	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	3	8	theme	dominated	594:602	arg1	mineralization					507:520	the mineralization	503:520	the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan	503:584	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	1	9	from	ash	136:138	arg1	China					180:184	China	180:184	China	180:184	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	7	10	theme	hydroxyl	1209:1216	arg1	Al-OH					1227:1231	Al-OH	1227:1231	Al-OH	1227:1231	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	10	theme	hydroxyl	1209:1216	arg1	group					1218:1222	the surface hydroxyl group	1197:1222	the surface hydroxyl group of Al-OH and -NH2	1197:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	10	theme	hydroxyl	1209:1216	arg1	As-O					1188:1191	As-O	1188:1191	As-O	1188:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	10	theme	hydroxyl	1209:1216	arg1	As-N					1179:1182	As-N	1179:1182	As-N	1179:1182	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	10	theme	hydroxyl	1209:1216	arg1	-NH2					1237:1240	-NH2	1237:1240	-NH2	1237:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	4	11	theme	coated	740:745	arg1	chitosan					747:754	the coated chitosan	736:754	the coated chitosan	736:754	From the batch experiments of As(V) removal, it has been found that the coated chitosan could significantly improve As(V) performance of Na-X zeolite.					
30756353	5	12	theme	optimal	823:829	arg1	pH					839:840	The optimal working pH	819:840	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite	819:890	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	1	13	theme	chitosan-coated	228:242	arg1	Na-X					244:247	chitosan-coated Na-X zeolite	228:255	chitosan-coated Na-X zeolite	228:255	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	5	14	theme	maximum	930:936	arg1	63.23 mg/g					962:971	63.23 mg/g	962:971	63.23 mg/g	962:971	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	5	14	theme	maximum	930:936	arg1	capacity					949:956	the maximum adsorption capacity	926:956	the maximum adsorption capacity	926:956	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	0	15	dep	As	96:97	arg1	V					99:99	V	99:99	V	99:99	Characterizations and mechanisms for synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study.					
30756353	4	16	theme	zeolite	810:816	arg1	Na-X					805:808	Na-X zeolite	805:816	Na-X zeolite	805:816	From the batch experiments of As(V) removal, it has been found that the coated chitosan could significantly improve As(V) performance of Na-X zeolite.					
30756353	3	17	theme	coated	618:623	arg1	chitosan					625:632	coated chitosan	618:632	coated chitosan	618:632	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	6	18	theme	different	1001:1009	arg1	time					1020:1023	different interval time	1001:1023	different interval time	1001:1023	And the adsorption data at different interval time was excellent fitted by pseudo-second-order kinetic model.					
30756353	1	19	theme	low	145:147	arg1	aluminum					149:156	low aluminum	145:156	low aluminum of Yunnan Province	145:175	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	3	20	theme	C-OH	569:572	arg1	Si-OH					530:534	Si-OH	530:534	Si-OH of the obtained Na-X zeolite and C-OH of chitosan	530:584	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	4	21	theme	Na-X	805:808	arg1	performance					790:800	(V) performance	786:800	(V) performance of Na-X zeolite	786:816	From the batch experiments of As(V) removal, it has been found that the coated chitosan could significantly improve As(V) performance of Na-X zeolite.					
30756353	3	22	theme	low	434:436	arg1	ash					451:453	the low aluminum fly ash	430:453	the low aluminum fly ash	430:453	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	4	23	dep	performance	790:800	arg1	V					787:787	V	787:787	V	787:787	From the batch experiments of As(V) removal, it has been found that the coated chitosan could significantly improve As(V) performance of Na-X zeolite.					
30756353	3	24	theme	Na-X	654:657	arg1	surface					643:649	the surface	639:649	the surface of Na-X zeolite	639:665	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	7	25	theme	acid	1277:1280	arg1	wastewater					1282:1291	acid wastewater	1277:1291	acid wastewater	1277:1291	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	3	26	theme	zeolite	490:496	arg1	Na-X					485:488	Na-X zeolite	485:496	Na-X zeolite	485:496	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	1	27	theme	zeolite	249:255	arg1	Na-X					244:247	chitosan-coated Na-X zeolite	228:255	chitosan-coated Na-X zeolite	228:255	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	0	28	theme	adsorption	102:111	arg1	study					113:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	Characterizations and mechanisms for synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study.					
30756353	5	29	theme	chitosan-coated	863:877	arg1	Na-X					879:882	chitosan-coated Na-X zeolite	863:890	chitosan-coated Na-X zeolite	863:890	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	6	30	theme	adsorption	982:991	arg1	data					993:996	the adsorption data	978:996	the adsorption data at different interval time	978:1023	And the adsorption data at different interval time was excellent fitted by pseudo-second-order kinetic model.					
30756353	3	31	theme	fly	447:449	arg1	ash					451:453	the low aluminum fly ash	430:453	the low aluminum fly ash	430:453	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	0	32	from	ash	88:90	arg1	zeolite					71:77	zeolite	71:77	zeolite	71:77	Characterizations and mechanisms for synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study.					
30756353	7	33	theme	surface	1201:1207	arg1	Al-OH					1227:1231	Al-OH	1227:1231	Al-OH	1227:1231	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	33	theme	surface	1201:1207	arg1	group					1218:1222	the surface hydroxyl group	1197:1222	the surface hydroxyl group of Al-OH and -NH2	1197:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	33	theme	surface	1201:1207	arg1	As-O					1188:1191	As-O	1188:1191	As-O	1188:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	33	theme	surface	1201:1207	arg1	As-N					1179:1182	As-N	1179:1182	As-N	1179:1182	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	33	theme	surface	1201:1207	arg1	-NH2					1237:1240	-NH2	1237:1240	-NH2	1237:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	1	34	theme	Province	168:175	arg1	aluminum					149:156	low aluminum	145:156	low aluminum of Yunnan Province	145:175	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	7	35	theme	As	1136:1137	arg1	uptake					1142:1147	As(V) uptake	1136:1147	As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2	1136:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	35	theme	As	1136:1137	arg1	V					1139:1139	V	1139:1139	V	1139:1139	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	36	theme	As-O	1188:1191	arg1	group					1218:1222	the surface hydroxyl group	1197:1222	the surface hydroxyl group of Al-OH and -NH2	1197:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	36	theme	As-O	1188:1191	arg1	As-N					1179:1182	As-N	1179:1182	As-N	1179:1182	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	36	theme	As-O	1188:1191	arg1	As-O					1188:1191	As-O	1188:1191	As-O	1188:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	36	theme	As-O	1188:1191	arg1	Al-OH					1227:1231	Al-OH	1227:1231	Al-OH	1227:1231	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	36	theme	As-O	1188:1191	arg1	-NH2					1237:1240	-NH2	1237:1240	-NH2	1237:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	36	theme	As-O	1188:1191	arg1	bond					1171:1174	the bond	1167:1174	the bond of As-N and As-O	1167:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	37	theme	-NH2	1237:1240	arg1	group					1218:1222	the surface hydroxyl group	1197:1222	the surface hydroxyl group of Al-OH and -NH2	1197:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	37	theme	-NH2	1237:1240	arg1	As-N					1179:1182	As-N	1179:1182	As-N	1179:1182	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	37	theme	-NH2	1237:1240	arg1	As-O					1188:1191	As-O	1188:1191	As-O	1188:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	37	theme	-NH2	1237:1240	arg1	Al-OH					1227:1231	Al-OH	1227:1231	Al-OH	1227:1231	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	37	theme	-NH2	1237:1240	arg1	-NH2					1237:1240	-NH2	1237:1240	-NH2	1237:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	37	theme	-NH2	1237:1240	arg1	bond					1171:1174	the bond	1167:1174	the bond of As-N and As-O	1167:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	0	38	theme	study	113:117	arg1	synthesis					37:45	synthesis	37:45	synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	37:117	Characterizations and mechanisms for synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study.					
30756353	7	39	dep	the	1089:1091	arg1	analyze					1093:1099	analyze	1093:1099	analyze	1093:1099	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	2	40	theme	prepared	337:344	arg1	materials					346:354	the prepared materials	333:354	the prepared materials	333:354	Then, the prepared materials were characterized by XRD, FT-IR, and XPS.					
30756353	0	41	theme	Na-X	66:69	arg1	study					113:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	Characterizations and mechanisms for synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study.					
30756353	1	42	theme	obtained	266:273	arg1	As					310:311	As	310:311	As	310:311	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	1	42	theme	obtained	266:273	arg1	material					285:292	the obtained composite material	262:292	the obtained composite material	262:292	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	3	43	theme	zeolite	659:665	arg1	Na-X					654:657	Na-X zeolite	654:665	Na-X zeolite	654:665	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	7	44	theme	As-N	1179:1182	arg1	group					1218:1222	the surface hydroxyl group	1197:1222	the surface hydroxyl group of Al-OH and -NH2	1197:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	44	theme	As-N	1179:1182	arg1	As-N					1179:1182	As-N	1179:1182	As-N	1179:1182	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	44	theme	As-N	1179:1182	arg1	As-O					1188:1191	As-O	1188:1191	As-O	1188:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	44	theme	As-N	1179:1182	arg1	Al-OH					1227:1231	Al-OH	1227:1231	Al-OH	1227:1231	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	44	theme	As-N	1179:1182	arg1	-NH2					1237:1240	-NH2	1237:1240	-NH2	1237:1240	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	7	44	theme	As-N	1179:1182	arg1	bond					1171:1174	the bond	1167:1174	the bond of As-N and As-O	1167:1191	From the analyze of XPS, the results suggested that As(V) uptake over adsorbent by the bond of As-N and As-O and the surface hydroxyl group of Al-OH and -NH2 were involved in uptake As(V) from acid wastewater.					
30756353	5	45	theme	adsorption	938:947	arg1	63.23 mg/g					962:971	63.23 mg/g	962:971	63.23 mg/g	962:971	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	5	45	theme	adsorption	938:947	arg1	capacity					949:956	the maximum adsorption capacity	926:956	the maximum adsorption capacity	926:956	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	0	46	theme	chitosan-coated	50:64	arg1	study					113:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	Characterizations and mechanisms for synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study.					
30756353	1	47	theme	composite	275:283	arg1	As					310:311	As	310:311	As	310:311	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	1	47	theme	composite	275:283	arg1	material					285:292	the obtained composite material	262:292	the obtained composite material	262:292	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	3	48	theme	chitosan	577:584	arg1	zeolite					557:563	zeolite	557:563	zeolite	557:563	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	4	49	theme	removal	704:710	arg1	experiments					683:693	the batch experiments	673:693	the batch experiments of As(V) removal	673:710	From the batch experiments of As(V) removal, it has been found that the coated chitosan could significantly improve As(V) performance of Na-X zeolite.					
30756353	6	50	theme	kinetic	1069:1075	arg1	model					1077:1081	pseudo-second-order kinetic model	1049:1081	pseudo-second-order kinetic model	1049:1081	And the adsorption data at different interval time was excellent fitted by pseudo-second-order kinetic model.					
30756353	5	51	theme	zeolite	884:890	arg1	Na-X					879:882	chitosan-coated Na-X zeolite	863:890	chitosan-coated Na-X zeolite	863:890	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	6	52	theme	pseudo-second-order	1049:1067	arg1	model					1077:1081	pseudo-second-order kinetic model	1049:1081	pseudo-second-order kinetic model	1049:1081	And the adsorption data at different interval time was excellent fitted by pseudo-second-order kinetic model.					
30756353	4	53	theme	batch	677:681	arg1	experiments					683:693	the batch experiments	673:693	the batch experiments of As(V) removal	673:710	From the batch experiments of As(V) removal, it has been found that the coated chitosan could significantly improve As(V) performance of Na-X zeolite.					
30756353	1	54	used	used	190:193	arg2	ash					136:138	Solid waste fly ash	120:138	Solid waste fly ash with low aluminum of Yunnan Province in China	120:184	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	1	54	used	used	190:193	arg2	material					207:214	pristine material	198:214	pristine material	198:214	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	6	55	theme	interval	1011:1018	arg1	time					1020:1023	different interval time	1001:1023	different interval time	1001:1023	And the adsorption data at different interval time was excellent fitted by pseudo-second-order kinetic model.					
30756353	3	56	theme	aluminum	438:445	arg1	ash					451:453	the low aluminum fly ash	430:453	the low aluminum fly ash	430:453	And the results suggested that the low aluminum fly ash was successfully convert into Na-X zeolite, and the mineralization between Si-OH of the obtained Na-X zeolite and C-OH of chitosan was the dominated mechanism for coated chitosan over the surface of Na-X zeolite.					
30756353	5	57	theme	working	831:837	arg1	pH					839:840	The optimal working pH	819:840	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite	819:890	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	5	58	theme	As	854:855	arg1	removal					846:852	removal As	846:855	removal As(V) by chitosan-coated Na-X zeolite	846:890	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	5	58	theme	As	854:855	arg1	V					857:857	V	857:857	V	857:857	The optimal working pH for removal As(V) by chitosan-coated Na-X zeolite was attained at pH 2.1 ± 0.1, and the maximum adsorption capacity was 63.23 mg/g.					
30756353	1	59	theme	Solid	120:124	arg1	material					207:214	pristine material	198:214	pristine material	198:214	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	1	59	theme	Solid	120:124	arg1	ash					136:138	Solid waste fly ash	120:138	Solid waste fly ash with low aluminum of Yunnan Province in China	120:184	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	4	60	dep	As	698:699	arg1	V					701:701	V	701:701	V	701:701	From the batch experiments of As(V) removal, it has been found that the coated chitosan could significantly improve As(V) performance of Na-X zeolite.					
30756353	0	61	theme	zeolite	71:77	arg1	study					113:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study	50:117	Characterizations and mechanisms for synthesis of chitosan-coated Na-X zeolite from fly ash and As(V) adsorption study.					
30756353	4	62	theme	As	698:699	arg1	removal					704:710	As(V) removal	698:710	As(V) removal	698:710	From the batch experiments of As(V) removal, it has been found that the coated chitosan could significantly improve As(V) performance of Na-X zeolite.					
30756353	1	63	theme	waste	126:130	arg1	material					207:214	pristine material	198:214	pristine material	198:214	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	1	63	theme	waste	126:130	arg1	ash					136:138	Solid waste fly ash	120:138	Solid waste fly ash with low aluminum of Yunnan Province in China	120:184	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	6	64	from	time	1020:1023	arg1	data					993:996	the adsorption data	978:996	the adsorption data at different interval time	978:1023	And the adsorption data at different interval time was excellent fitted by pseudo-second-order kinetic model.					
30756353	1	65	theme	pristine	198:205	arg1	material					207:214	pristine material	198:214	pristine material	198:214	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30756353	1	65	theme	pristine	198:205	arg1	ash					136:138	Solid waste fly ash	120:138	Solid waste fly ash with low aluminum of Yunnan Province in China	120:184	Solid waste fly ash with low aluminum of Yunnan Province in China was used as pristine material to prepared chitosan-coated Na-X zeolite, and the obtained composite material was employed as As(V) adsorbent.					
30940587	7	0	dep	explained	998:1006	arg1	wherein					1075:1081	wherein	1075:1081	wherein the chromium sorption process was best fitted with Langmuir isotherm	1075:1150	The obtained equilibrium data was explained with Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms wherein the chromium sorption process was best fitted with Langmuir isotherm.					
30940587	9	1	theme	n-GO	1291:1294	arg1	biocomposite					1301:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite could be easily regenerated with 0.1 M NaOH.					
30940587	2	2	dep	oxide	293:297	arg1	composite					340:348	fabricated hydrotalcite (n-GO@HT) composite	306:348	nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite	279:348	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	7	3	theme	obtained	968:975	arg1	data					989:992	The obtained equilibrium data	964:992	The obtained equilibrium data	964:992	The obtained equilibrium data was explained with Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms wherein the chromium sorption process was best fitted with Langmuir isotherm.					
30940587	0	4	theme	adsorbent	93:101	arg1	removal					116:122	An efficient adsorbent for chromium removal	80:122	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.	0:134	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.					
30940587	10	5	theme	HTCS	1380:1383	arg1	biocomposite					1385:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite was also utilized in the field conditions.					
30940587	8	6	theme	thermodynamic	1157:1169	arg1	results					1171:1177	The thermodynamic results	1153:1177	The thermodynamic results	1153:1177	The thermodynamic results prove that the chromium sorption of n-GO@HTCS biocomposite was endothermic and spontaneous.					
30940587	7	7	theme	Langmuir	1134:1141	arg1	isotherm					1143:1150	Langmuir isotherm	1134:1150	Langmuir isotherm	1134:1150	The obtained equilibrium data was explained with Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms wherein the chromium sorption process was best fitted with Langmuir isotherm.					
30940587	7	8	theme	Dubinin-Radushkevich	1038:1057	arg1	isotherms					1065:1073	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1013:1073	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1013:1073	The obtained equilibrium data was explained with Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms wherein the chromium sorption process was best fitted with Langmuir isotherm.					
30940587	4	9	theme	various	633:639	arg1	TEM					681:683	TEM	681:683	TEM	681:683	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	4	9	theme	various	633:639	arg1	EDAX					689:692	EDAX	689:692	EDAX	689:692	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	4	9	theme	various	633:639	arg1	SEM					676:678	SEM	676:678	SEM	676:678	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	4	9	theme	various	633:639	arg1	techniques					654:663	various instrumental techniques	633:663	various instrumental techniques like FTIR	633:673	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	4	10	theme	mapping	699:705	arg1	analysis					707:714	mapping analysis	699:714	mapping analysis	699:714	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	10	11	theme	n-GO	1375:1378	arg1	biocomposite					1385:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite was also utilized in the field conditions.					
30940587	2	12	theme	nano-graphene	279:291	arg1	n-GO					300:303	n-GO	300:303	n-GO	300:303	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	2	12	theme	nano-graphene	279:291	arg1	oxide					293:297	nano-graphene oxide	279:297	nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite	279:348	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	1	13	theme	nanomaterials	146:158	arg1	adsorbents					166:175	nanomaterials based adsorbents	146:175	nanomaterials based adsorbents	146:175	Recently, nanomaterials based adsorbents play a prominent role in the removal of toxic ions from aqueous solution.					
30940587	3	14	theme	GO	474:475	arg1	composite					480:488	GO@HT composite	474:488	GO@HT composite	474:488	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	7	15	theme	Langmuir	1025:1032	arg1	isotherms					1065:1073	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1013:1073	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1013:1073	The obtained equilibrium data was explained with Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms wherein the chromium sorption process was best fitted with Langmuir isotherm.					
30940587	4	16	theme	synthesized	584:594	arg1	biocomposite					596:607	The synthesized biocomposite	580:607	The synthesized biocomposite	580:607	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	1	17	theme	prominent	184:192	arg1	role					194:197	a prominent role	182:197	a prominent role	182:197	Recently, nanomaterials based adsorbents play a prominent role in the removal of toxic ions from aqueous solution.					
30940587	6	18	theme	biocomposite	909:920	arg1	SC					892:893	SC	892:893	SC	892:893	The sorption capacity (SC) of n-GO@HTCS biocomposite was found to be 42.64 mg/g within 50 min.					
30940587	6	18	theme	biocomposite	909:920	arg1	capacity					882:889	The sorption capacity	869:889	The sorption capacity (SC) of n-GO@HTCS biocomposite	869:920	The sorption capacity (SC) of n-GO@HTCS biocomposite was found to be 42.64 mg/g within 50 min.					
30940587	5	19	theme	chromium	736:743	arg1	retention					745:753	the maximum chromium retention	724:753	the maximum chromium retention	724:753	To get the maximum chromium retention, the various sorption experiments like agitation time, dosage, pH, competing ions and temperature were optimized.					
30940587	10	20	theme	synthesized	1363:1373	arg1	biocomposite					1385:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite was also utilized in the field conditions.					
30940587	2	21	theme	hydrothermal	366:377	arg1	method					379:384	hydrothermal method	366:384	hydrothermal method for chromium removal	366:405	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	5	22	theme	various	760:766	arg1	experiments					777:787	the various sorption experiments	756:787	the various sorption experiments like agitation time, dosage, pH, competing ions and temperature	756:851	To get the maximum chromium retention, the various sorption experiments like agitation time, dosage, pH, competing ions and temperature were optimized.					
30940587	5	23	theme	maximum	728:734	arg1	retention					745:753	the maximum chromium retention	724:753	the maximum chromium retention	724:753	To get the maximum chromium retention, the various sorption experiments like agitation time, dosage, pH, competing ions and temperature were optimized.					
30940587	7	24	theme	Freundlich	1013:1022	arg1	isotherms					1065:1073	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1013:1073	Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms	1013:1073	The obtained equilibrium data was explained with Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms wherein the chromium sorption process was best fitted with Langmuir isotherm.					
30940587	9	25	theme	chromium	1275:1282	arg1	biocomposite					1301:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite could be easily regenerated with 0.1 M NaOH.					
30940587	2	26	theme	fabricated	306:315	arg1	composite					340:348	fabricated hydrotalcite (n-GO@HT) composite	306:348	nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite	279:348	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	8	27	theme	chromium	1194:1201	arg1	endothermic					1242:1252	endothermic	1242:1252	endothermic	1242:1252	The thermodynamic results prove that the chromium sorption of n-GO@HTCS biocomposite was endothermic and spontaneous.					
30940587	8	27	theme	chromium	1194:1201	arg1	sorption					1203:1210	the chromium sorption	1190:1210	the chromium sorption of n-GO@HTCS biocomposite	1190:1236	The thermodynamic results prove that the chromium sorption of n-GO@HTCS biocomposite was endothermic and spontaneous.					
30940587	5	28	theme	sorption	768:775	arg1	experiments					777:787	the various sorption experiments	756:787	the various sorption experiments like agitation time, dosage, pH, competing ions and temperature	756:851	To get the maximum chromium retention, the various sorption experiments like agitation time, dosage, pH, competing ions and temperature were optimized.					
30940587	6	29	theme	HTCS	904:907	arg1	biocomposite					909:920	n-GO@HTCS biocomposite	899:920	n-GO@HTCS biocomposite	899:920	The sorption capacity (SC) of n-GO@HTCS biocomposite was found to be 42.64 mg/g within 50 min.					
30940587	3	30	theme	hybrid	532:537	arg1	biocomposite					566:577	hybrid composite namely n-GO@HTCS biocomposite	532:577	hybrid composite namely n-GO@HTCS biocomposite	532:577	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	4	31	theme	instrumental	641:652	arg1	TEM					681:683	TEM	681:683	TEM	681:683	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	4	31	theme	instrumental	641:652	arg1	EDAX					689:692	EDAX	689:692	EDAX	689:692	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	4	31	theme	instrumental	641:652	arg1	SEM					676:678	SEM	676:678	SEM	676:678	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	4	31	theme	instrumental	641:652	arg1	techniques					654:663	various instrumental techniques	633:663	various instrumental techniques like FTIR	633:673	The synthesized biocomposite was characterized using various instrumental techniques like FTIR, SEM, TEM and EDAX with mapping analysis.					
30940587	0	32	theme	oxide	29:33	arg1	biocomposite					66:77	nano-graphene oxide assisted hydrotalcite/chitosan biocomposite	15:77	nano-graphene oxide assisted hydrotalcite/chitosan biocomposite	15:77	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.					
30940587	6	33	theme	@	903:903	arg1	biocomposite					909:920	n-GO@HTCS biocomposite	899:920	n-GO@HTCS biocomposite	899:920	The sorption capacity (SC) of n-GO@HTCS biocomposite was found to be 42.64 mg/g within 50 min.					
30940587	10	34	theme	@	1379:1379	arg1	biocomposite					1385:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite was also utilized in the field conditions.					
30940587	3	35	theme	chromium	447:454	arg1	capacity					464:471	chromium removal capacity	447:471	chromium removal capacity	447:471	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	0	36	theme	nano-graphene	15:27	arg1	biocomposite					66:77	nano-graphene oxide assisted hydrotalcite/chitosan biocomposite	15:77	nano-graphene oxide assisted hydrotalcite/chitosan biocomposite	15:77	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.					
30940587	6	37	theme	n-GO	899:902	arg1	biocomposite					909:920	n-GO@HTCS biocomposite	899:920	n-GO@HTCS biocomposite	899:920	The sorption capacity (SC) of n-GO@HTCS biocomposite was found to be 42.64 mg/g within 50 min.					
30940587	9	38	theme	@	1295:1295	arg1	biocomposite					1301:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite could be easily regenerated with 0.1 M NaOH.					
30940587	3	39	theme	removal	456:462	arg1	capacity					464:471	chromium removal capacity	447:471	chromium removal capacity	447:471	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	0	40	theme	hydrotalcite/chitosan	44:64	arg1	biocomposite					66:77	nano-graphene oxide assisted hydrotalcite/chitosan biocomposite	15:77	nano-graphene oxide assisted hydrotalcite/chitosan biocomposite	15:77	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.					
30940587	3	41	theme	@	476:476	arg1	composite					480:488	GO@HT composite	474:488	GO@HT composite	474:488	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	10	42	theme	field	1423:1427	arg1	conditions					1429:1438	the field conditions	1419:1438	the field conditions	1419:1438	The synthesized n-GO@HTCS biocomposite was also utilized in the field conditions.					
30940587	1	43	theme	toxic	217:221	arg1	ions					223:226	toxic ions	217:226	toxic ions	217:226	Recently, nanomaterials based adsorbents play a prominent role in the removal of toxic ions from aqueous solution.					
30940587	3	44	theme	n-GO	556:559	arg1	HTCS					561:564	namely n-GO@HTCS	549:564	hybrid composite namely n-GO@HTCS biocomposite	532:577	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	5	45	theme	competing	822:830	arg1	ions					832:835	competing ions	822:835	competing ions	822:835	To get the maximum chromium retention, the various sorption experiments like agitation time, dosage, pH, competing ions and temperature were optimized.					
30940587	2	46	theme	chromium	390:397	arg1	removal					399:405	chromium removal	390:405	chromium removal	390:405	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	0	47	theme	assisted	35:42	arg1	biocomposite					66:77	nano-graphene oxide assisted hydrotalcite/chitosan biocomposite	15:77	nano-graphene oxide assisted hydrotalcite/chitosan biocomposite	15:77	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.					
30940587	9	48	theme	HTCS	1296:1299	arg1	biocomposite					1301:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite could be easily regenerated with 0.1 M NaOH.					
30940587	7	49	theme	chromium	1087:1094	arg1	process					1105:1111	the chromium sorption process	1083:1111	the chromium sorption process	1083:1111	The obtained equilibrium data was explained with Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms wherein the chromium sorption process was best fitted with Langmuir isotherm.					
30940587	8	50	theme	@	1219:1219	arg1	biocomposite					1225:1236	n-GO@HTCS biocomposite	1215:1236	n-GO@HTCS biocomposite	1215:1236	The thermodynamic results prove that the chromium sorption of n-GO@HTCS biocomposite was endothermic and spontaneous.					
30940587	3	51	theme	@	560:560	arg1	HTCS					561:564	namely n-GO@HTCS	549:564	hybrid composite namely n-GO@HTCS biocomposite	532:577	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	1	52	theme	ions	223:226	arg1	removal					206:212	the removal	202:212	the removal of toxic ions from aqueous solution	202:248	Recently, nanomaterials based adsorbents play a prominent role in the removal of toxic ions from aqueous solution.					
30940587	8	53	theme	biocomposite	1225:1236	arg1	endothermic					1242:1252	endothermic	1242:1252	endothermic	1242:1252	The thermodynamic results prove that the chromium sorption of n-GO@HTCS biocomposite was endothermic and spontaneous.					
30940587	8	53	theme	biocomposite	1225:1236	arg1	sorption					1203:1210	the chromium sorption	1190:1210	the chromium sorption of n-GO@HTCS biocomposite	1190:1236	The thermodynamic results prove that the chromium sorption of n-GO@HTCS biocomposite was endothermic and spontaneous.					
30940587	3	54	theme	HT	477:478	arg1	composite					480:488	GO@HT composite	474:488	GO@HT composite	474:488	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	1	55	from	solution	241:248	arg1	removal					206:212	the removal	202:212	the removal of toxic ions from aqueous solution	202:248	Recently, nanomaterials based adsorbents play a prominent role in the removal of toxic ions from aqueous solution.					
30940587	9	56	theme	sorbed	1284:1289	arg1	biocomposite					1301:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite	1271:1312	The chromium sorbed n-GO@HTCS biocomposite could be easily regenerated with 0.1 M NaOH.					
30940587	3	57	theme	composite	539:547	arg1	biocomposite					566:577	hybrid composite namely n-GO@HTCS biocomposite	532:577	hybrid composite namely n-GO@HTCS biocomposite	532:577	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	2	58	theme	@	335:335	arg1	hydrotalcite					317:328	hydrotalcite	317:328	nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite	279:348	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	2	58	theme	@	335:335	arg1	HT					336:337	n-GO@HT	331:337	n-GO@HT	331:337	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	0	59	theme	biocomposite	66:77	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.	0:134	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.					
30940587	7	60	theme	equilibrium	977:987	arg1	data					989:992	The obtained equilibrium data	964:992	The obtained equilibrium data	964:992	The obtained equilibrium data was explained with Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms wherein the chromium sorption process was best fitted with Langmuir isotherm.					
30940587	0	61	from	water	129:133	arg1	removal					116:122	An efficient adsorbent for chromium removal	80:122	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.	0:134	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.					
30940587	2	62	theme	n-GO	331:334	arg1	hydrotalcite					317:328	hydrotalcite	317:328	nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite	279:348	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	2	62	theme	n-GO	331:334	arg1	HT					336:337	n-GO@HT	331:337	n-GO@HT	331:337	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	7	63	theme	sorption	1096:1103	arg1	process					1105:1111	the chromium sorption process	1083:1111	the chromium sorption process	1083:1111	The obtained equilibrium data was explained with Freundlich, Langmuir and Dubinin-Radushkevich (D-R) isotherms wherein the chromium sorption process was best fitted with Langmuir isotherm.					
30940587	10	64	used	utilized	1407:1414	arg2	biocomposite					1385:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite	1359:1396	The synthesized n-GO@HTCS biocomposite was also utilized in the field conditions.					
30940587	2	65	theme	present	264:270	arg1	study					272:276	the present study	260:276	the present study	260:276	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	2	66	theme	hydrotalcite	317:328	arg1	composite					340:348	fabricated hydrotalcite (n-GO@HT) composite	306:348	nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite	279:348	Hence in the present study, nano-graphene oxide (n-GO) fabricated hydrotalcite (n-GO@HT) composite was prepared by hydrothermal method for chromium removal.					
30940587	1	67	theme	aqueous	233:239	arg1	solution					241:248	aqueous solution	233:248	aqueous solution	233:248	Recently, nanomaterials based adsorbents play a prominent role in the removal of toxic ions from aqueous solution.					
30940587	0	68	theme	efficient	83:91	arg1	removal					116:122	An efficient adsorbent for chromium removal	80:122	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.	0:134	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.					
30940587	8	69	theme	n-GO	1215:1218	arg1	biocomposite					1225:1236	n-GO@HTCS biocomposite	1215:1236	n-GO@HTCS biocomposite	1215:1236	The thermodynamic results prove that the chromium sorption of n-GO@HTCS biocomposite was endothermic and spontaneous.					
30940587	3	70	theme	HTCS	561:564	arg1	biocomposite					566:577	hybrid composite namely n-GO@HTCS biocomposite	532:577	hybrid composite namely n-GO@HTCS biocomposite	532:577	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	5	71	theme	agitation	794:802	arg1	time					804:807	agitation time	794:807	agitation time	794:807	To get the maximum chromium retention, the various sorption experiments like agitation time, dosage, pH, competing ions and temperature were optimized.					
30940587	1	72	theme	based	160:164	arg1	adsorbents					166:175	nanomaterials based adsorbents	146:175	nanomaterials based adsorbents	146:175	Recently, nanomaterials based adsorbents play a prominent role in the removal of toxic ions from aqueous solution.					
30940587	3	73	theme	mechanical	423:432	arg1	strength					434:441	the mechanical strength	419:441	the mechanical strength	419:441	To improve the mechanical strength and chromium removal capacity, GO@HT composite was reinforced with chitosan (CS) to form hybrid composite namely n-GO@HTCS biocomposite.					
30940587	6	74	theme	sorption	873:880	arg1	SC					892:893	SC	892:893	SC	892:893	The sorption capacity (SC) of n-GO@HTCS biocomposite was found to be 42.64 mg/g within 50 min.					
30940587	6	74	theme	sorption	873:880	arg1	capacity					882:889	The sorption capacity	869:889	The sorption capacity (SC) of n-GO@HTCS biocomposite	869:920	The sorption capacity (SC) of n-GO@HTCS biocomposite was found to be 42.64 mg/g within 50 min.					
30940587	0	75	dep	Fabrication	0:10	arg1	removal					116:122	An efficient adsorbent for chromium removal	80:122	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.	0:134	Fabrication of nano-graphene oxide assisted hydrotalcite/chitosan biocomposite: An efficient adsorbent for chromium removal from water.					
30940587	8	76	theme	HTCS	1220:1223	arg1	biocomposite					1225:1236	n-GO@HTCS biocomposite	1215:1236	n-GO@HTCS biocomposite	1215:1236	The thermodynamic results prove that the chromium sorption of n-GO@HTCS biocomposite was endothermic and spontaneous.					
29649519	7	0	theme	Apparatus	1448:1456	arg1	port					1470:1473	the Apparatus E induction port	1444:1473	the Apparatus E induction port	1444:1473	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	4	1	theme	diameter	834:841	arg1	terms					813:817	terms	813:817	terms of aerodynamic diameter	813:841	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	8	2	from	concentration	1516:1528	arg1	blends					1537:1542	the blends	1533:1542	the blends	1533:1542	CONCLUSION As the BDP concentration in the blends increases, aerosol performance of the formulation decreases, in an inversely proportional manner.					
29649519	2	3	theme	carrier	536:542	arg1	system					544:549	a multi-component carrier system	518:549	a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate	518:616	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	2	3	theme	carrier	536:542	arg1	formulations					392:403	METHODS Five formulations	379:403	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w)	379:497	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	6	4	theme	individual	1137:1146	arg1	agglomerates					1148:1159	individual agglomerates	1137:1159	individual agglomerates	1137:1159	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	6	5	theme	carrier	1227:1233	arg1	surface					1204:1210	the surface	1200:1210	the surface of the lactose carrier	1200:1233	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	7	6	theme	BDP	1422:1424	arg1	deposition					1426:1435	BDP deposition	1422:1435	BDP deposition within the Apparatus E induction port	1422:1473	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	2	7	theme	fine	581:584	arg1	lactose					586:592	fine lactose	581:592	fine lactose	581:592	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	3	8	theme	component	662:670	arg1	morphology					623:632	The morphology	619:632	The morphology of the formulation and each component	619:670	The morphology of the formulation and each component were investigated using scanning electron micrographs while the particle size was measured by laser diffraction.					
29649519	10	9	from	effect	1859:1864	arg1	behaviour					1895:1903	the behaviour	1891:1903	the behaviour of aerosol dispersion	1891:1925	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	10	10	theme	dispersion	1916:1925	arg1	behaviour					1895:1903	the behaviour	1891:1903	the behaviour of aerosol dispersion	1891:1925	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	2	11	with	lactose	569:575	arg1	stearate					609:616	magnesium stearate	599:616	magnesium stearate	599:616	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	9	12	dep	particles	1778:1786	arg1	BDP					1789:1791	BDP	1789:1791	BDP	1789:1791	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	12	dep	particles	1778:1786	arg1	particles					1778:1786	micronized particles	1767:1786	micronized particles (BDP and Micronized composite carrier)	1767:1825	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	12	dep	particles	1778:1786	arg1	carrier					1818:1824	Micronized composite carrier	1797:1824	Micronized composite carrier	1797:1824	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	4	13	theme	Apparatus	888:896	arg1	impactor					908:915	the British pharmacopeia Apparatus E cascade impactor	863:915	the British pharmacopeia Apparatus E cascade impactor (Next generation impactor)	863:942	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	4	13	theme	Apparatus	888:896	arg1	impactor					934:941	Next generation impactor	918:941	Next generation impactor	918:941	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	3	14	theme	scanning	696:703	arg1	micrographs					714:724	scanning electron micrographs	696:724	scanning electron micrographs while the particle size was measured by laser diffraction	696:782	The morphology of the formulation and each component were investigated using scanning electron micrographs while the particle size was measured by laser diffraction.					
29649519	4	15	theme	cascade	900:906	arg1	impactor					908:915	the British pharmacopeia Apparatus E cascade impactor	863:915	the British pharmacopeia Apparatus E cascade impactor (Next generation impactor)	863:942	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	4	15	theme	cascade	900:906	arg1	impactor					934:941	Next generation impactor	918:941	Next generation impactor	918:941	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	10	16	dep	development	1975:1985	arg1	the					1971:1973	the	1971:1973	the	1971:1973	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	4	17	from	performance	797:807	arg1	terms					813:817	terms	813:817	terms of aerodynamic diameter	813:841	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	7	18	theme	dense	1327:1331	arg1	agglomerates					1333:1344	dense agglomerates	1327:1344	dense agglomerates	1327:1344	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	9	19	theme	induction	1696:1704	arg1	port					1706:1709	the induction port	1692:1709	the induction port	1692:1709	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	5	20	theme	performance	995:1005	arg1	chromatography					1014:1027	high performance liquid chromatography	990:1027	high performance liquid chromatography (HPLC)	990:1034	Chemical analysis of the API was observed by high performance liquid chromatography (HPLC).					
29649519	5	20	theme	performance	995:1005	arg1	HPLC					1030:1033	HPLC	1030:1033	HPLC	1030:1033	Chemical analysis of the API was observed by high performance liquid chromatography (HPLC).					
29649519	4	21	theme	British	867:873	arg1	impactor					908:915	the British pharmacopeia Apparatus E cascade impactor	863:915	the British pharmacopeia Apparatus E cascade impactor (Next generation impactor)	863:942	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	4	21	theme	British	867:873	arg1	impactor					934:941	Next generation impactor	918:941	Next generation impactor	918:941	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	6	22	theme	packed	1173:1178	arg1	multi-layers					1184:1195	densely packed BDP multi-layers	1165:1195	densely packed BDP multi-layers on the surface of the lactose carrier	1165:1233	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	1	23	dep	PURPOSE	53:59	arg1	performed					76:84	performed	76:84	was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig	72:376	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	3	24	theme	laser	766:770	arg1	diffraction					772:782	laser diffraction	766:782	laser diffraction	766:782	The morphology of the formulation and each component were investigated using scanning electron micrographs while the particle size was measured by laser diffraction.					
29649519	9	25	from	formulation	1842:1852	arg1	present					1827:1833	present	1827:1833	present	1827:1833	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	1	26	theme	pharmaceutical	127:140	arg1	API					154:156	API	154:156	API	154:156	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	1	26	theme	pharmaceutical	127:140	arg1	ingredient					142:151	the active pharmaceutical ingredient	116:151	the active pharmaceutical ingredient (API) content	116:165	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	9	27	from	port	1706:1709	arg1	percentage					1660:1669	the percentage	1656:1669	the percentage of API deposition in the induction port and pre-separator	1656:1727	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	7	28	theme	primary	1302:1308	arg1	particles					1310:1318	individual primary particles	1291:1318	individual primary particles	1291:1318	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	2	29	contain	containing	551:560	arg1	system					544:549	a multi-component carrier system	518:549	a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate	518:616	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	2	29	contain	containing	551:560	arg2	lactose					586:592	fine lactose	581:592	fine lactose	581:592	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	2	29	contain	containing	551:560	arg2	lactose					569:575	coarse lactose	562:575	coarse lactose	562:575	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	2	29	contain	containing	551:560	arg1	formulations					392:403	METHODS Five formulations	379:403	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w)	379:497	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	2	30	with	lactose	586:592	arg1	stearate					609:616	magnesium stearate	599:616	magnesium stearate	599:616	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	1	31	theme	formulation	309:319	arg1	efficiency					252:261	the aerosol performance efficiency	228:261	the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation	228:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	1	31	theme	formulation	309:319	arg1	dispersion					200:209	the dispersion	196:209	the dispersion of particles	196:222	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	3	32	dep	micrographs	714:724	arg1	measured					754:761	measured	754:761	was measured by laser diffraction	750:782	The morphology of the formulation and each component were investigated using scanning electron micrographs while the particle size was measured by laser diffraction.					
29649519	9	33	from	deposition	1678:1687	arg1	port					1706:1709	the induction port	1692:1709	the induction port	1692:1709	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	2	34	theme	magnesium	599:607	arg1	stearate					609:616	magnesium stearate	599:616	magnesium stearate	599:616	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	1	35	theme	custom	330:335	arg1	rig					374:376	a custom dry powder inhaler (DPI) development rig	328:376	a custom dry powder inhaler (DPI) development rig	328:376	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	4	36	theme	generation	923:932	arg1	impactor					908:915	the British pharmacopeia Apparatus E cascade impactor	863:915	the British pharmacopeia Apparatus E cascade impactor (Next generation impactor)	863:942	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	4	36	theme	generation	923:932	arg1	impactor					934:941	Next generation impactor	918:941	Next generation impactor	918:941	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	1	37	theme	powder	341:346	arg1	rig					374:376	a custom dry powder inhaler (DPI) development rig	328:376	a custom dry powder inhaler (DPI) development rig	328:376	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	5	38	theme	Chemical	945:952	arg1	analysis					954:961	Chemical analysis	945:961	Chemical analysis of the API	945:972	Chemical analysis of the API was observed by high performance liquid chromatography (HPLC).					
29649519	7	39	theme	individual	1291:1300	arg1	particles					1310:1318	individual primary particles	1291:1318	individual primary particles	1291:1318	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	1	40	theme	carrier	268:274	arg1	formulation					309:319	a carrier based dry powder inhalable (DPI) formulation	266:319	a carrier based dry powder inhalable (DPI) formulation	266:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	9	41	theme	micronized	1767:1776	arg1	BDP					1789:1791	BDP	1789:1791	BDP	1789:1791	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	41	theme	micronized	1767:1776	arg1	particles					1778:1786	micronized particles	1767:1786	micronized particles (BDP and Micronized composite carrier)	1767:1825	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	41	theme	micronized	1767:1776	arg1	carrier					1818:1824	Micronized composite carrier	1797:1824	Micronized composite carrier	1797:1824	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	0	42	theme	high	15:18	arg1	carrier					25:31	high dose carrier	15:31	high dose carrier	15:31	Limitations of high dose carrier based formulations.					
29649519	1	43	theme	dry	282:284	arg1	formulation					309:319	a carrier based dry powder inhalable (DPI) formulation	266:319	a carrier based dry powder inhalable (DPI) formulation	266:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	2	44	theme	dipropionate	455:466	arg1	concentrations					418:431	varying concentrations	410:431	varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w)	410:497	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	0	45	theme	carrier	25:31	arg1	Limitations					0:10	Limitations	0:10	Limitations of high dose carrier	0:31	Limitations of high dose carrier based formulations.					
29649519	1	46	theme	inhalable	293:301	arg1	formulation					309:319	a carrier based dry powder inhalable (DPI) formulation	266:319	a carrier based dry powder inhalable (DPI) formulation	266:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	2	47	theme	API	436:438	arg1	BDP					469:471	BDP	469:471	BDP	469:471	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	2	47	theme	API	436:438	arg1	dipropionate					455:466	API beclomethasone dipropionate	436:466	API beclomethasone dipropionate (BDP) between 1% and 30% (w/w)	436:497	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	10	48	theme	increase	1879:1886	arg1	effect					1859:1864	The effect	1855:1864	The effect of such dose increase on the behaviour of aerosol dispersion	1855:1925	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	1	49	theme	DPI	304:306	arg1	formulation					309:319	a carrier based dry powder inhalable (DPI) formulation	266:319	a carrier based dry powder inhalable (DPI) formulation	266:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	5	50	theme	high	990:993	arg1	chromatography					1014:1027	high performance liquid chromatography	990:1027	high performance liquid chromatography (HPLC)	990:1034	Chemical analysis of the API was observed by high performance liquid chromatography (HPLC).					
29649519	5	50	theme	high	990:993	arg1	HPLC					1030:1033	HPLC	1030:1033	HPLC	1030:1033	Chemical analysis of the API was observed by high performance liquid chromatography (HPLC).					
29649519	7	51	theme	present	1240:1246	arg1	BDP					1236:1238	BDP	1236:1238	BDP present within the multi-layer	1236:1269	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	9	52	theme	composite	1808:1816	arg1	particles					1778:1786	micronized particles	1767:1786	micronized particles (BDP and Micronized composite carrier)	1767:1825	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	52	theme	composite	1808:1816	arg1	carrier					1818:1824	Micronized composite carrier	1797:1824	Micronized composite carrier	1797:1824	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	1	53	theme	particles	214:222	arg1	efficiency					252:261	the aerosol performance efficiency	228:261	the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation	228:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	1	53	theme	particles	214:222	arg1	dispersion					200:209	the dispersion	196:209	the dispersion of particles	196:222	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	7	54	theme	E	1458:1458	arg1	port					1470:1473	the Apparatus E induction port	1444:1473	the Apparatus E induction port	1444:1473	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	6	55	theme	BDP	1077:1079	arg1	concentration					1060:1072	the concentration	1056:1072	the concentration of BDP in the blend	1056:1092	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	10	56	theme	dosed	2014:2018	arg1	formulations					2034:2045	higher dosed carrier-based formulations	2007:2045	higher dosed carrier-based formulations	2007:2045	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	8	57	theme	aerosol	1555:1561	arg1	performance					1563:1573	aerosol performance	1555:1573	aerosol performance of the formulation	1555:1592	CONCLUSION As the BDP concentration in the blends increases, aerosol performance of the formulation decreases, in an inversely proportional manner.					
29649519	8	58	theme	proportional	1621:1632	arg1	manner					1634:1639	an inversely proportional manner	1608:1639	an inversely proportional manner	1608:1639	CONCLUSION As the BDP concentration in the blends increases, aerosol performance of the formulation decreases, in an inversely proportional manner.					
29649519	5	59	theme	API	970:972	arg1	analysis					954:961	Chemical analysis	945:961	Chemical analysis of the API	945:972	Chemical analysis of the API was observed by high performance liquid chromatography (HPLC).					
29649519	6	60	from	concentration	1060:1072	arg1	blend					1088:1092	the blend	1084:1092	the blend	1084:1092	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	1	61	theme	performance	240:250	arg1	efficiency					252:261	the aerosol performance efficiency	228:261	the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation	228:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	7	62	theme	deposition	1426:1435	arg1	percentage					1408:1417	percentage	1408:1417	percentage of BDP deposition within the Apparatus E induction port	1408:1473	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	7	62	theme	deposition	1426:1435	arg1	pre-separator					1479:1491	pre-separator	1479:1491	pre-separator	1479:1491	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	4	63	theme	aerodynamic	822:832	arg1	diameter					834:841	aerodynamic diameter	822:841	aerodynamic diameter	822:841	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	8	64	theme	formulation	1582:1592	arg1	performance					1563:1573	aerosol performance	1555:1573	aerosol performance of the formulation	1555:1592	CONCLUSION As the BDP concentration in the blends increases, aerosol performance of the formulation decreases, in an inversely proportional manner.					
29649519	3	65	theme	formulation	641:651	arg1	morphology					623:632	The morphology	619:632	The morphology of the formulation and each component	619:670	The morphology of the formulation and each component were investigated using scanning electron micrographs while the particle size was measured by laser diffraction.					
29649519	2	66	theme	multi-component	520:534	arg1	system					544:549	a multi-component carrier system	518:549	a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate	518:616	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	2	66	theme	multi-component	520:534	arg1	formulations					392:403	METHODS Five formulations	379:403	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w)	379:497	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	9	67	theme	API	1674:1676	arg1	deposition					1678:1687	API deposition	1674:1687	API deposition in the induction port	1674:1709	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	6	68	theme	lactose	1219:1225	arg1	carrier					1227:1233	the lactose carrier	1215:1233	the lactose carrier	1215:1233	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	9	69	theme	Micronized	1797:1806	arg1	particles					1778:1786	micronized particles	1767:1786	micronized particles (BDP and Micronized composite carrier)	1767:1825	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	69	theme	Micronized	1797:1806	arg1	carrier					1818:1824	Micronized composite carrier	1797:1824	Micronized composite carrier	1797:1824	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	7	70	theme	aerosol	1374:1380	arg1	performance					1382:1392	aerosol performance	1374:1392	aerosol performance	1374:1392	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	10	71	theme	dose	1874:1877	arg1	increase					1879:1886	such dose increase	1869:1886	such dose increase	1869:1886	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	9	72	attach	linked	1743:1748	arg2	percentage					1660:1669	the percentage	1656:1669	the percentage of API deposition in the induction port and pre-separator	1656:1727	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	72	attach	linked	1743:1748	arg1	amount					1757:1762	the amount	1753:1762	the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation	1753:1852	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	72	attach	linked	1743:1748	arg1	particles					1778:1786	micronized particles	1767:1786	micronized particles (BDP and Micronized composite carrier)	1767:1825	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	72	attach	linked	1743:1748	arg1	carrier					1818:1824	Micronized composite carrier	1797:1824	Micronized composite carrier	1797:1824	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	72	attach	linked	1743:1748	arg1	BDP					1789:1791	BDP	1789:1791	BDP	1789:1791	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	2	73	theme	coarse	562:567	arg1	lactose					569:575	coarse lactose	562:575	coarse lactose	562:575	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	9	74	from	present	1827:1833	arg1	formulation					1842:1852	the formulation	1838:1852	the formulation	1838:1852	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	10	75	theme	aerosol	1908:1914	arg1	dispersion					1916:1925	aerosol dispersion	1908:1925	aerosol dispersion	1908:1925	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	4	76	theme	pharmacopeia	875:886	arg1	impactor					908:915	the British pharmacopeia Apparatus E cascade impactor	863:915	the British pharmacopeia Apparatus E cascade impactor (Next generation impactor)	863:942	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	4	76	theme	pharmacopeia	875:886	arg1	impactor					934:941	Next generation impactor	918:941	Next generation impactor	918:941	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	3	77	theme	electron	705:712	arg1	micrographs					714:724	scanning electron micrographs	696:724	scanning electron micrographs while the particle size was measured by laser diffraction	696:782	The morphology of the formulation and each component were investigated using scanning electron micrographs while the particle size was measured by laser diffraction.					
29649519	1	78	theme	DPI	357:359	arg1	rig					374:376	a custom dry powder inhaler (DPI) development rig	328:376	a custom dry powder inhaler (DPI) development rig	328:376	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	10	79	theme	formulations	2034:2045	arg1	optimisation					1991:2002	optimisation	1991:2002	optimisation	1991:2002	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	10	79	theme	formulations	2034:2045	arg1	development					1975:1985	development	1975:1985	development	1975:1985	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	4	80	theme	E	898:898	arg1	impactor					908:915	the British pharmacopeia Apparatus E cascade impactor	863:915	the British pharmacopeia Apparatus E cascade impactor (Next generation impactor)	863:942	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	4	80	theme	E	898:898	arg1	impactor					934:941	Next generation impactor	918:941	Next generation impactor	918:941	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	1	81	theme	development	362:372	arg1	rig					374:376	a custom dry powder inhaler (DPI) development rig	328:376	a custom dry powder inhaler (DPI) development rig	328:376	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	2	82	with	formulations	392:403	arg1	concentrations					418:431	varying concentrations	410:431	varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w)	410:497	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	6	83	theme	BDP	1180:1182	arg1	multi-layers					1184:1195	densely packed BDP multi-layers	1165:1195	densely packed BDP multi-layers on the surface of the lactose carrier	1165:1233	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	6	84	from	multi-layers	1184:1195	arg1	surface					1204:1210	the surface	1200:1210	the surface of the lactose carrier	1200:1233	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	3	85	theme	particle	736:743	arg1	size					745:748	the particle size	732:748	the particle size	732:748	The morphology of the formulation and each component were investigated using scanning electron micrographs while the particle size was measured by laser diffraction.					
29649519	5	86	theme	liquid	1007:1012	arg1	chromatography					1014:1027	high performance liquid chromatography	990:1027	high performance liquid chromatography (HPLC)	990:1034	Chemical analysis of the API was observed by high performance liquid chromatography (HPLC).					
29649519	5	86	theme	liquid	1007:1012	arg1	HPLC					1030:1033	HPLC	1030:1033	HPLC	1030:1033	Chemical analysis of the API was observed by high performance liquid chromatography (HPLC).					
29649519	1	87	theme	active	120:125	arg1	API					154:156	API	154:156	API	154:156	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	1	87	theme	active	120:125	arg1	ingredient					142:151	the active pharmaceutical ingredient	116:151	the active pharmaceutical ingredient (API) content	116:165	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	1	88	theme	ingredient	142:151	arg1	content					159:165	the active pharmaceutical ingredient (API) content	116:165	the active pharmaceutical ingredient (API) content	116:165	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	7	89	from	decrease	1362:1369	arg1	performance					1382:1392	aerosol performance	1374:1392	aerosol performance	1374:1392	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	9	90	theme	pre-separator	1715:1727	arg1	percentage					1660:1669	the percentage	1656:1669	the percentage of API deposition in the induction port and pre-separator	1656:1727	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	6	91	theme	agglomerates	1148:1159	arg1	size					1129:1132	size	1129:1132	size	1129:1132	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	6	91	theme	agglomerates	1148:1159	arg1	numbers					1117:1123	numbers	1117:1123	numbers	1117:1123	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	6	91	theme	agglomerates	1148:1159	arg1	multi-layers					1184:1195	densely packed BDP multi-layers	1165:1195	densely packed BDP multi-layers on the surface of the lactose carrier	1165:1233	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	10	92	theme	greater	1952:1958	arg1	insight					1960:1966	greater insight	1952:1966	greater insight in the development and optimisation of higher dosed carrier-based formulations	1952:2045	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	9	93	from	percentage	1660:1669	arg1	port					1706:1709	the induction port	1692:1709	the induction port	1692:1709	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	6	94	from	size	1129:1132	arg1	surface					1204:1210	the surface	1200:1210	the surface of the lactose carrier	1200:1233	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	1	95	theme	dry	337:339	arg1	rig					374:376	a custom dry powder inhaler (DPI) development rig	328:376	a custom dry powder inhaler (DPI) development rig	328:376	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	4	96	theme	Next	918:921	arg1	impactor					908:915	the British pharmacopeia Apparatus E cascade impactor	863:915	the British pharmacopeia Apparatus E cascade impactor (Next generation impactor)	863:942	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	4	96	theme	Next	918:921	arg1	impactor					934:941	Next generation impactor	918:941	Next generation impactor	918:941	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	1	97	theme	inhaler	348:354	arg1	rig					374:376	a custom dry powder inhaler (DPI) development rig	328:376	a custom dry powder inhaler (DPI) development rig	328:376	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	2	98	theme	varying	410:416	arg1	concentrations					418:431	varying concentrations	410:431	varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w)	410:497	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	8	99	theme	BDP	1512:1514	arg1	concentration					1516:1528	the BDP concentration	1508:1528	the BDP concentration in the blends	1508:1542	CONCLUSION As the BDP concentration in the blends increases, aerosol performance of the formulation decreases, in an inversely proportional manner.					
29649519	9	100	from	pre-separator	1715:1727	arg1	port					1706:1709	the induction port	1692:1709	the induction port	1692:1709	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	0	101	theme	dose	20:23	arg1	carrier					25:31	high dose carrier	15:31	high dose carrier	15:31	Limitations of high dose carrier based formulations.					
29649519	1	102	theme	based	276:280	arg1	formulation					309:319	a carrier based dry powder inhalable (DPI) formulation	266:319	a carrier based dry powder inhalable (DPI) formulation	266:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	10	103	from	insight	1960:1966	arg1	optimisation					1991:2002	optimisation	1991:2002	optimisation	1991:2002	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	10	103	from	insight	1960:1966	arg1	development					1975:1985	development	1975:1985	development	1975:1985	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	9	104	theme	particles	1778:1786	arg1	BDP					1789:1791	BDP	1789:1791	BDP	1789:1791	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	104	theme	particles	1778:1786	arg1	amount					1757:1762	the amount	1753:1762	the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation	1753:1852	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	104	theme	particles	1778:1786	arg1	particles					1778:1786	micronized particles	1767:1786	micronized particles (BDP and Micronized composite carrier)	1767:1825	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	104	theme	particles	1778:1786	arg1	carrier					1818:1824	Micronized composite carrier	1797:1824	Micronized composite carrier	1797:1824	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	6	105	from	numbers	1117:1123	arg1	surface					1204:1210	the surface	1200:1210	the surface of the lactose carrier	1200:1233	RESULTS Increasing the concentration of BDP in the blend resulted in increasing numbers and size of individual agglomerates and densely packed BDP multi-layers on the surface of the lactose carrier.					
29649519	2	106	theme	METHODS	379:385	arg1	system					544:549	a multi-component carrier system	518:549	a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate	518:616	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	2	106	theme	METHODS	379:385	arg1	formulations					392:403	METHODS Five formulations	379:403	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w)	379:497	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	1	107	theme	powder	286:291	arg1	formulation					309:319	a carrier based dry powder inhalable (DPI) formulation	266:319	a carrier based dry powder inhalable (DPI) formulation	266:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
29649519	10	108	theme	higher	2007:2012	arg1	formulations					2034:2045	higher dosed carrier-based formulations	2007:2045	higher dosed carrier-based formulations	2007:2045	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	2	109	theme	beclomethasone	440:453	arg1	BDP					469:471	BDP	469:471	BDP	469:471	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	2	109	theme	beclomethasone	440:453	arg1	dipropionate					455:466	API beclomethasone dipropionate	436:466	API beclomethasone dipropionate (BDP) between 1% and 30% (w/w)	436:497	METHODS Five formulations with varying concentrations of API beclomethasone dipropionate (BDP) between 1% and 30% (w/w) were formulated as a multi-component carrier system containing coarse lactose and fine lactose with magnesium stearate.					
29649519	9	110	attach	present	1827:1833	arg2	amount					1757:1762	the amount	1753:1762	the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation	1753:1852	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	110	attach	present	1827:1833	arg2	BDP					1789:1791	BDP	1789:1791	BDP	1789:1791	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	110	attach	present	1827:1833	arg1	formulation					1842:1852	the formulation	1838:1852	the formulation	1838:1852	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	110	attach	present	1827:1833	arg2	carrier					1818:1824	Micronized composite carrier	1797:1824	Micronized composite carrier	1797:1824	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	110	attach	present	1827:1833	arg2	particles					1778:1786	micronized particles	1767:1786	micronized particles (BDP and Micronized composite carrier)	1767:1825	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	111	theme	deposition	1678:1687	arg1	percentage					1660:1669	the percentage	1656:1669	the percentage of API deposition in the induction port and pre-separator	1656:1727	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	4	112	theme	aerosol	789:795	arg1	performance					797:807	The aerosol performance	785:807	The aerosol performance	785:807	The aerosol performance, in terms of aerodynamic diameter, was assessed using the British pharmacopeia Apparatus E cascade impactor (Next generation impactor).					
29649519	10	113	theme	carrier-based	2020:2032	arg1	formulations					2034:2045	higher dosed carrier-based formulations	2007:2045	higher dosed carrier-based formulations	2007:2045	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	7	114	theme	induction	1460:1468	arg1	port					1470:1473	the Apparatus E induction port	1444:1473	the Apparatus E induction port	1444:1473	BDP present within the multi-layer did not disperse as individual primary particles but as dense agglomerates, which led to a decrease in aerosol performance and increased percentage of BDP deposition within the Apparatus E induction port and pre-separator.					
29649519	9	115	theme	present	1827:1833	arg1	BDP					1789:1791	BDP	1789:1791	BDP	1789:1791	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	115	theme	present	1827:1833	arg1	amount					1757:1762	the amount	1753:1762	the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation	1753:1852	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	115	theme	present	1827:1833	arg1	particles					1778:1786	micronized particles	1767:1786	micronized particles (BDP and Micronized composite carrier)	1767:1825	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	9	115	theme	present	1827:1833	arg1	carrier					1818:1824	Micronized composite carrier	1797:1824	Micronized composite carrier	1797:1824	Concurrently, the percentage of API deposition in the induction port and pre-separator could also be linked to the amount of micronized particles (BDP and Micronized composite carrier) present in the formulation.					
29649519	10	116	theme	such	1869:1872	arg1	increase					1879:1886	such dose increase	1869:1886	such dose increase	1869:1886	The effect of such dose increase on the behaviour of aerosol dispersion was investigated to gain greater insight in the development and optimisation of higher dosed carrier-based formulations.					
29649519	1	117	theme	aerosol	232:238	arg1	efficiency					252:261	the aerosol performance efficiency	228:261	the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation	228:319	PURPOSE This study was performed to investigate how increasing the active pharmaceutical ingredient (API) content within a formulation affects the dispersion of particles and the aerosol performance efficiency of a carrier based dry powder inhalable (DPI) formulation, using a custom dry powder inhaler (DPI) development rig.					
31731553	8	0	theme	OB	997:998	arg1	salt-tolerant					1033:1045	salt-tolerant	1033:1045	salt-tolerant	1033:1045	The OB emulsions stabilized by ALG were salt-tolerant and freeze-thaw resistant.					
31731553	8	0	theme	OB	997:998	arg1	emulsions					1000:1008	The OB emulsions	993:1008	The OB emulsions stabilized by ALG	993:1026	The OB emulsions stabilized by ALG were salt-tolerant and freeze-thaw resistant.					
31731553	0	1	from	Stability	14:22	arg1	Seeds					65:69	Diverse Plant Seeds	51:69	Diverse Plant Seeds Using Sodium Alginate	51:91	Improving the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate.					
31731553	4	2	theme	different	431:439	arg1	emulsions					444:452	different OB emulsions	431:452	different OB emulsions	431:452	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	4	3	theme	endogenous	496:505	arg1	proteins					521:528	endogenous and exogenous proteins	496:528	endogenous and exogenous proteins	496:528	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	6	4	used	used	665:668	arg2	alginate					646:653	Sodium alginate	639:653	Sodium alginate (ALG)	639:659	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	6	4	used	used	665:668	arg2	ALG					656:658	ALG	656:658	ALG	656:658	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	7	5	theme	viscosity	967:975	arg1	effect					977:982	the viscosity effect	963:982	the viscosity effect	963:982	While a high concentration of ALG improved the OB emulsion stability through the viscosity effect at pH 7.					
31731553	4	6	contain	contained	454:462	arg2	contents					484:491	contents	484:491	contents	484:491	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	4	6	contain	contained	454:462	arg1	emulsions					444:452	different OB emulsions	431:452	different OB emulsions	431:452	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	4	6	contain	contained	454:462	arg2	types					474:478	types	474:478	types	474:478	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	4	7	theme	proteins	521:528	arg1	contents					484:491	contents	484:491	contents	484:491	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	4	7	theme	proteins	521:528	arg1	types					474:478	types	474:478	types	474:478	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	0	8	theme	Sodium	77:82	arg1	Alginate					84:91	Sodium Alginate	77:91	Sodium Alginate	77:91	Improving the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate.					
31731553	5	9	theme	high	575:578	arg1	7					585:585	7	585:585	7	585:585	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions.					
31731553	5	9	theme	high	575:578	arg1	pHs					580:582	high pHs	575:582	high pHs (7 and 8)	575:592	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions.					
31731553	5	9	theme	high	575:578	arg1	8					591:591	8	591:591	8	591:591	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions.					
31731553	4	10	theme	exogenous	511:519	arg1	proteins					521:528	endogenous and exogenous proteins	496:528	endogenous and exogenous proteins	496:528	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	6	11	theme	ALG	759:761	arg1	concentration					742:754	different conditions-low concentration	717:754	different conditions-low concentration of ALG	717:761	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	3	12	theme	emulsions	385:393	arg1	stability					365:373	The stability	361:373	The stability of the OB emulsions	361:393	The stability of the OB emulsions was investigated.					
31731553	1	13	theme	rapeseed	129:136	arg1	bodies					142:147	rapeseed oil bodies	129:147	rapeseed oil bodies (OBs)	129:153	In this study, peanut, sesame, and rapeseed oil bodies (OBs) were extracted by the aqueous medium method.					
31731553	1	13	theme	rapeseed	129:136	arg1	OBs					150:152	OBs	150:152	OBs	150:152	In this study, peanut, sesame, and rapeseed oil bodies (OBs) were extracted by the aqueous medium method.					
31731553	2	14	theme	protein	212:218	arg1	microstructure					233:246	microstructure	233:246	microstructure	233:246	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	14	theme	protein	212:218	arg1	&nbsp					277:281	&nbsp; 3	277:284	&nbsp; 3	277:284	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	14	theme	protein	212:218	arg1	composition					220:230	The surface protein composition	200:230	The surface protein composition	200:230	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	14	theme	protein	212:218	arg1	size					266:269	average particle size	249:269	average particle size	249:269	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	15	theme	extracted	307:315	arg1	OBs					317:319	the extracted OBs	303:319	the extracted OBs in aqueous emulsion	303:339	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	7	16	theme	emulsion	936:943	arg1	stability					945:953	the OB emulsion stability	929:953	the OB emulsion stability	929:953	While a high concentration of ALG improved the OB emulsion stability through the viscosity effect at pH 7.					
31731553	5	17	theme	OB	625:626	arg1	emulsions					628:636	three OB emulsions	619:636	three OB emulsions	619:636	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions.					
31731553	6	18	theme	isoelectric	821:831	arg1	point					833:837	the isoelectric point	817:837	the isoelectric point of the OBs	817:848	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	2	19	theme	particle	257:264	arg1	composition					220:230	The surface protein composition	200:230	The surface protein composition	200:230	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	19	theme	particle	257:264	arg1	size					266:269	average particle size	249:269	average particle size	249:269	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	6	20	theme	different	717:725	arg1	concentration					742:754	different conditions-low concentration	717:754	different conditions-low concentration of ALG	717:761	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	5	21	theme	low	546:548	arg1	<6					555:556	<6	555:556	<6	555:556	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions.					
31731553	5	21	theme	low	546:548	arg1	pHs					550:552	low pHs	546:552	low pHs (<6)	546:557	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions.					
31731553	2	22	theme	average	249:255	arg1	composition					220:230	The surface protein composition	200:230	The surface protein composition	200:230	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	22	theme	average	249:255	arg1	size					266:269	average particle size	249:269	average particle size	249:269	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	3	23	theme	OB	382:383	arg1	emulsions					385:393	the OB emulsions	378:393	the OB emulsions	378:393	The stability of the OB emulsions was investigated.					
31731553	5	24	from	pHs	550:552	arg1	Aggregation					531:541	Aggregation	531:541	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8)	531:592	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions.					
31731553	7	25	theme	OB	933:934	arg1	stability					945:953	the OB emulsion stability	929:953	the OB emulsion stability	929:953	While a high concentration of ALG improved the OB emulsion stability through the viscosity effect at pH 7.					
31731553	6	26	theme	conditions-low	727:740	arg1	concentration					742:754	different conditions-low concentration	717:754	different conditions-low concentration of ALG	717:761	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	2	27	dep	composition	220:230	arg1	d					271:271	d 4	271:273	d 4	271:273	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	0	28	theme	Body	31:34	arg1	Emulsions					36:44	Oil Body Emulsions	27:44	Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate	27:91	Improving the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate.					
31731553	7	29	theme	ALG	916:918	arg1	concentration					899:911	a high concentration	892:911	a high concentration of ALG	892:918	While a high concentration of ALG improved the OB emulsion stability through the viscosity effect at pH 7.					
31731553	0	30	from	Seeds	65:69	arg1	Stability					14:22	the Stability	10:22	the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate	10:91	Improving the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate.					
31731553	0	30	from	Seeds	65:69	arg1	Emulsions					36:44	Oil Body Emulsions	27:44	Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate	27:91	Improving the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate.					
31731553	2	31	theme	aqueous	324:330	arg1	emulsion					332:339	aqueous emulsion	324:339	aqueous emulsion	324:339	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	32	theme	OBs	317:319	arg1	ζ-potential					288:298	ζ-potential	288:298	ζ-potential	288:298	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	0	33	theme	Oil	27:29	arg1	Emulsions					36:44	Oil Body Emulsions	27:44	Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate	27:91	Improving the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate.					
31731553	5	34	from	pHs	580:582	arg1	Aggregation					531:541	Aggregation	531:541	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8)	531:592	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions.					
31731553	6	35	theme	Sodium	639:644	arg1	ALG					656:658	ALG	656:658	ALG	656:658	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	6	35	theme	Sodium	639:644	arg1	alginate					646:653	Sodium alginate	639:653	Sodium alginate (ALG)	639:659	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	6	36	theme	emulsions	792:800	arg1	stability					776:784	the stability	772:784	the stability of OB emulsions	772:800	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	4	37	theme	OB	441:442	arg1	emulsions					444:452	different OB emulsions	431:452	different OB emulsions	431:452	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	6	38	theme	emulsions	701:709	arg1	instability					683:693	the instability	679:693	the instability of OB emulsions	679:709	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	0	39	theme	Emulsions	36:44	arg1	Stability					14:22	the Stability	10:22	the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate	10:91	Improving the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate.					
31731553	4	40	theme	different	464:472	arg1	types					474:478	types	474:478	types	474:478	It was found that different OB emulsions contained different types and contents of endogenous and exogenous proteins.					
31731553	6	41	theme	OB	698:699	arg1	emulsions					701:709	OB emulsions	698:709	OB emulsions	698:709	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	2	42	from	OBs	317:319	arg1	emulsion					332:339	aqueous emulsion	324:339	aqueous emulsion	324:339	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	5	43	from	creaming	563:570	arg1	Aggregation					531:541	Aggregation	531:541	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8)	531:592	Aggregation at low pHs (<6) and creaming at high pHs (7 and 8) both occurred for all of three OB emulsions.					
31731553	2	44	theme	surface	204:210	arg1	microstructure					233:246	microstructure	233:246	microstructure	233:246	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	44	theme	surface	204:210	arg1	&nbsp					277:281	&nbsp; 3	277:284	&nbsp; 3	277:284	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	44	theme	surface	204:210	arg1	composition					220:230	The surface protein composition	200:230	The surface protein composition	200:230	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	44	theme	surface	204:210	arg1	size					266:269	average particle size	249:269	average particle size	249:269	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	6	45	theme	OBs	846:848	arg1	point					833:837	the isoelectric point	817:837	the isoelectric point of the OBs	817:848	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	7	46	theme	high	894:897	arg1	concentration					899:911	a high concentration	892:911	a high concentration of ALG	892:918	While a high concentration of ALG improved the OB emulsion stability through the viscosity effect at pH 7.					
31731553	0	47	theme	Plant	59:63	arg1	Seeds					65:69	Diverse Plant Seeds	51:69	Diverse Plant Seeds Using Sodium Alginate	51:91	Improving the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate.					
31731553	2	48	theme	ζ-potential	288:298	arg1	microstructure					233:246	microstructure	233:246	microstructure	233:246	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	48	theme	ζ-potential	288:298	arg1	&nbsp					277:281	&nbsp; 3	277:284	&nbsp; 3	277:284	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	48	theme	ζ-potential	288:298	arg1	composition					220:230	The surface protein composition	200:230	The surface protein composition	200:230	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	2	48	theme	ζ-potential	288:298	arg1	size					266:269	average particle size	249:269	average particle size	249:269	The surface protein composition, microstructure, average particle size d 4 , &nbsp; 3 , ζ-potential of the extracted OBs in aqueous emulsion were characterized.					
31731553	1	49	theme	oil	138:140	arg1	bodies					142:147	rapeseed oil bodies	129:147	rapeseed oil bodies (OBs)	129:153	In this study, peanut, sesame, and rapeseed oil bodies (OBs) were extracted by the aqueous medium method.					
31731553	1	49	theme	oil	138:140	arg1	OBs					150:152	OBs	150:152	OBs	150:152	In this study, peanut, sesame, and rapeseed oil bodies (OBs) were extracted by the aqueous medium method.					
31731553	1	50	theme	aqueous	177:183	arg1	method					192:197	the aqueous medium method	173:197	the aqueous medium method	173:197	In this study, peanut, sesame, and rapeseed oil bodies (OBs) were extracted by the aqueous medium method.					
31731553	0	51	theme	Diverse	51:57	arg1	Seeds					65:69	Diverse Plant Seeds	51:69	Diverse Plant Seeds Using Sodium Alginate	51:91	Improving the Stability of Oil Body Emulsions from Diverse Plant Seeds Using Sodium Alginate.					
31731553	6	52	theme	OB	789:790	arg1	emulsions					792:800	OB emulsions	789:800	OB emulsions	789:800	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	6	53	theme	electrostatic	859:871	arg1	interaction					873:883	electrostatic interaction	859:883	electrostatic interaction	859:883	Sodium alginate (ALG) was used to solve the instability of OB emulsions under different conditions-low concentration of ALG improved the stability of OB emulsions below and near the isoelectric point of the OBs, through electrostatic interaction.					
31731553	1	54	theme	medium	185:190	arg1	method					192:197	the aqueous medium method	173:197	the aqueous medium method	173:197	In this study, peanut, sesame, and rapeseed oil bodies (OBs) were extracted by the aqueous medium method.					
30594356	2	0	theme	fresh	417:421	arg1	milk					429:432	fresh human milk	417:432	fresh human milk	417:432	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	7	1	theme	Fore	1044:1047	arg1	milk					1049:1052	Fore milk	1044:1052	Fore milk	1044:1052	Fore milk was significantly less creamy, less fatty, thinner, more watery, and lower in vanilla flavor intensity than hind milk.					
30594356	5	2	theme	fresh	794:798	arg1	milk					800:803	human fresh milk	788:803	human fresh milk	788:803	The odor of human fresh milk was described as neutral, creamy, and sweet, taste as sweet and bitter, and mouthfeel as thin, watery, smooth, and fatty.					
30594356	1	3	theme	human	220:224	arg1	milk					226:229	fresh human milk	214:229	fresh human milk	214:229	It is unknown how consumption of bitter foods and beverages in the maternal diet influences sensory properties of fresh human milk.					
30594356	2	4	theme	diet	505:508	arg1	milk					520:523	human milk	514:523	human milk	514:523	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	2	4	theme	diet	505:508	arg1	bitterness					478:487	bitterness	478:487	bitterness of the maternal diet	478:508	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	10	5	theme	foods	1474:1478	arg1	consumption					1452:1462	the consumption	1448:1462	the consumption of bitter foods	1448:1478	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	1	6	theme	bitter	133:138	arg1	foods					140:144	bitter foods	133:144	bitter foods	133:144	It is unknown how consumption of bitter foods and beverages in the maternal diet influences sensory properties of fresh human milk.					
30594356	1	7	theme	milk	226:229	arg1	properties					200:209	sensory properties	192:209	sensory properties of fresh human milk	192:229	It is unknown how consumption of bitter foods and beverages in the maternal diet influences sensory properties of fresh human milk.					
30594356	7	8	theme	vanilla	1132:1138	arg1	intensity					1147:1155	vanilla flavor intensity	1132:1155	vanilla flavor intensity	1132:1155	Fore milk was significantly less creamy, less fatty, thinner, more watery, and lower in vanilla flavor intensity than hind milk.					
30594356	2	9	theme	maternal	496:503	arg1	diet					505:508	the maternal diet	492:508	the maternal diet	492:508	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	2	10	theme	study	249:253	arg1	aims					236:239	The aims	232:239	The aims of this study	232:253	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	6	11	theme	mL	977:978	arg1	g					960:960	1.53 g	955:960	1.53 g of sucrose/100 mL	955:978	Sweetness was equivalent to 1.53 g of sucrose/100 mL and was not significantly different between fore and hind milk.					
30594356	8	12	theme	Carbohydrate	1173:1184	arg1	content					1186:1192	Carbohydrate content	1173:1192	Carbohydrate content of human milk	1173:1206	Carbohydrate content of human milk was positively correlated with sweetness and glutamic acid content with umami.					
30594356	9	13	theme	fore	1401:1404	arg1	milk					1406:1409	fore milk	1401:1409	fore milk	1401:1409	The bitterness of the diet consumed 24 h before lactation was moderately positively correlated with bitterness of fore milk, but not hind milk.					
30594356	7	14	theme	less	1072:1075	arg1	creamy					1077:1082	significantly less creamy	1058:1082	significantly less creamy	1058:1082	Fore milk was significantly less creamy, less fatty, thinner, more watery, and lower in vanilla flavor intensity than hind milk.					
30594356	3	15	theme	sensory	641:647	arg1	rating					669:674	sensory attribute intensity rating	641:674	sensory attribute intensity rating	641:674	Twenty-two lactating mothers generated sensory terms to describe perception of their milk and received training on sensory attribute intensity rating.					
30594356	2	16	dep	establish	347:355	arg1	2					341:341	2	341:341	2	341:341	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	1	17	from	consumption	118:128	arg1	diet					176:179	the maternal diet	163:179	the maternal diet	163:179	It is unknown how consumption of bitter foods and beverages in the maternal diet influences sensory properties of fresh human milk.					
30594356	4	18	theme	24-h	692:695	arg1	diary					702:706	a 24-h food diary	690:706	a 24-h food diary followed by a sensory self-assessment of their fore and hind milks	690:773	Mothers kept a 24-h food diary followed by a sensory self-assessment of their fore and hind milks.					
30594356	7	19	theme	lower	1123:1127	arg1	creamy					1077:1082	significantly less creamy	1058:1082	significantly less creamy	1058:1082	Fore milk was significantly less creamy, less fatty, thinner, more watery, and lower in vanilla flavor intensity than hind milk.					
30594356	1	20	theme	foods	140:144	arg1	consumption					118:128	consumption	118:128	consumption of bitter foods and beverages in the maternal diet	118:179	It is unknown how consumption of bitter foods and beverages in the maternal diet influences sensory properties of fresh human milk.					
30594356	0	21	theme	bitter	86:91	arg1	taste					93:97	bitter taste	86:97	bitter taste	86:97	Sensory characteristics of human milk: Association between mothers' diet and milk for bitter taste.					
30594356	5	22	theme	milk	800:803	arg1	mouthfeel					881:889	mouthfeel	881:889	mouthfeel	881:889	The odor of human fresh milk was described as neutral, creamy, and sweet, taste as sweet and bitter, and mouthfeel as thin, watery, smooth, and fatty.					
30594356	5	22	theme	milk	800:803	arg1	taste					850:854	taste	850:854	taste as sweet and bitter	850:874	The odor of human fresh milk was described as neutral, creamy, and sweet, taste as sweet and bitter, and mouthfeel as thin, watery, smooth, and fatty.					
30594356	5	22	theme	milk	800:803	arg1	creamy					831:836	creamy	831:836	creamy	831:836	The odor of human fresh milk was described as neutral, creamy, and sweet, taste as sweet and bitter, and mouthfeel as thin, watery, smooth, and fatty.					
30594356	5	22	theme	milk	800:803	arg1	sweet					843:847	sweet	843:847	sweet	843:847	The odor of human fresh milk was described as neutral, creamy, and sweet, taste as sweet and bitter, and mouthfeel as thin, watery, smooth, and fatty.					
30594356	5	22	theme	milk	800:803	arg1	odor					780:783	The odor	776:783	The odor of human fresh milk	776:803	The odor of human fresh milk was described as neutral, creamy, and sweet, taste as sweet and bitter, and mouthfeel as thin, watery, smooth, and fatty.					
30594356	9	23	theme	milk	1425:1428	arg1	bitterness					1387:1396	bitterness	1387:1396	bitterness of fore milk, but not hind milk	1387:1428	The bitterness of the diet consumed 24 h before lactation was moderately positively correlated with bitterness of fore milk, but not hind milk.					
30594356	2	24	theme	hind	329:332	arg1	milk					334:337	fresh human fore and hind milk	308:337	milk	334:337	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	4	25	theme	hind	764:767	arg1	milks					769:773	their fore and hind milks	749:773	their fore and hind milks	749:773	Mothers kept a 24-h food diary followed by a sensory self-assessment of their fore and hind milks.					
30594356	2	26	dep	determine	267:275	arg1	1					261:261	1	261:261	1	261:261	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	1	27	theme	beverages	150:158	arg1	consumption					118:128	consumption	118:128	consumption of bitter foods and beverages in the maternal diet	118:179	It is unknown how consumption of bitter foods and beverages in the maternal diet influences sensory properties of fresh human milk.					
30594356	0	28	theme	Sensory	0:6	arg1	characteristics					8:22	Sensory characteristics	0:22	Sensory characteristics of human milk: Association between mothers' diet and milk for bitter taste.	0:98	Sensory characteristics of human milk: Association between mothers' diet and milk for bitter taste.					
30594356	2	29	theme	human	514:518	arg1	milk					520:523	human milk	514:523	human milk	514:523	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	4	30	theme	fore	755:758	arg1	milks					769:773	their fore and hind milks	749:773	their fore and hind milks	749:773	Mothers kept a 24-h food diary followed by a sensory self-assessment of their fore and hind milks.					
30594356	2	31	theme	fore	320:323	arg1	characteristics					289:303	the sensory characteristics	277:303	the sensory characteristics of fresh human fore and hind milk	277:337	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	0	32	theme	human	27:31	arg1	milk					33:36	human milk	27:36	human milk	27:36	Sensory characteristics of human milk: Association between mothers' diet and milk for bitter taste.					
30594356	6	33	theme	hind	1033:1036	arg1	milk					1038:1041	hind milk	1033:1041	hind milk	1033:1041	Sweetness was equivalent to 1.53 g of sucrose/100 mL and was not significantly different between fore and hind milk.					
30594356	4	34	theme	milks	769:773	arg1	self-assessment					730:744	self-assessment	730:744	self-assessment	730:744	Mothers kept a 24-h food diary followed by a sensory self-assessment of their fore and hind milks.					
30594356	9	35	theme	milk	1406:1409	arg1	bitterness					1387:1396	bitterness	1387:1396	bitterness of fore milk, but not hind milk	1387:1428	The bitterness of the diet consumed 24 h before lactation was moderately positively correlated with bitterness of fore milk, but not hind milk.					
30594356	10	36	dep	influence	1484:1492	arg1	way					1550:1552	another way for breastfed children to learn to accept bitter vegetables	1542:1612	another way for breastfed children to learn to accept bitter vegetables	1542:1612	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	3	37	theme	attribute	649:657	arg1	rating					669:674	sensory attribute intensity rating	641:674	sensory attribute intensity rating	641:674	Twenty-two lactating mothers generated sensory terms to describe perception of their milk and received training on sensory attribute intensity rating.					
30594356	8	38	theme	acid	1262:1265	arg1	content					1267:1273	glutamic acid content	1253:1273	glutamic acid content	1253:1273	Carbohydrate content of human milk was positively correlated with sweetness and glutamic acid content with umami.					
30594356	0	39	dep	mothers	59:65	arg1	diet					68:71	diet	68:71	diet	68:71	Sensory characteristics of human milk: Association between mothers' diet and milk for bitter taste.					
30594356	10	40	theme	fore	1518:1521	arg1	milk					1523:1526	human fore milk	1512:1526	human fore milk	1512:1526	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	1	41	theme	maternal	167:174	arg1	diet					176:179	the maternal diet	163:179	the maternal diet	163:179	It is unknown how consumption of bitter foods and beverages in the maternal diet influences sensory properties of fresh human milk.					
30594356	7	42	theme	watery	1111:1116	arg1	creamy					1077:1082	significantly less creamy	1058:1082	significantly less creamy	1058:1082	Fore milk was significantly less creamy, less fatty, thinner, more watery, and lower in vanilla flavor intensity than hind milk.					
30594356	3	43	theme	intensity	659:667	arg1	rating					669:674	sensory attribute intensity rating	641:674	sensory attribute intensity rating	641:674	Twenty-two lactating mothers generated sensory terms to describe perception of their milk and received training on sensory attribute intensity rating.					
30594356	3	44	theme	sensory	565:571	arg1	terms					573:577	sensory terms	565:577	sensory terms	565:577	Twenty-two lactating mothers generated sensory terms to describe perception of their milk and received training on sensory attribute intensity rating.					
30594356	8	45	theme	milk	1203:1206	arg1	content					1186:1192	Carbohydrate content	1173:1192	Carbohydrate content of human milk	1173:1206	Carbohydrate content of human milk was positively correlated with sweetness and glutamic acid content with umami.					
30594356	10	46	theme	food	1644:1647	arg1	preferences					1649:1659	healthier food preferences	1634:1659	healthier food preferences	1634:1659	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	2	47	theme	milk	334:337	arg1	characteristics					289:303	the sensory characteristics	277:303	the sensory characteristics of fresh human fore and hind milk	277:337	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	0	48	theme	milk	33:36	arg1	characteristics					8:22	Sensory characteristics	0:22	Sensory characteristics of human milk: Association between mothers' diet and milk for bitter taste.	0:98	Sensory characteristics of human milk: Association between mothers' diet and milk for bitter taste.					
30594356	3	49	theme	lactating	537:545	arg1	mothers					547:553	Twenty-two lactating mothers	526:553	Twenty-two lactating mothers	526:553	Twenty-two lactating mothers generated sensory terms to describe perception of their milk and received training on sensory attribute intensity rating.					
30594356	8	50	theme	human	1197:1201	arg1	milk					1203:1206	human milk	1197:1206	human milk	1197:1206	Carbohydrate content of human milk was positively correlated with sweetness and glutamic acid content with umami.					
30594356	2	51	dep	assess	446:451	arg1	3					440:440	3	440:440	3	440:440	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	10	52	theme	milk	1523:1526	arg1	bitterness					1498:1507	the bitterness	1494:1507	the bitterness of human fore milk	1494:1526	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	2	53	theme	sensory	281:287	arg1	characteristics					289:303	the sensory characteristics	277:303	the sensory characteristics of fresh human fore and hind milk	277:337	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	2	54	theme	sensory	379:385	arg1	properties					387:396	sensory properties	379:396	sensory properties	379:396	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	10	55	theme	healthier	1634:1642	arg1	preferences					1649:1659	healthier food preferences	1634:1659	healthier food preferences	1634:1659	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	10	56	theme	bitter	1467:1472	arg1	foods					1474:1478	bitter foods	1467:1478	bitter foods	1467:1478	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	7	57	theme	thinner	1097:1103	arg1	creamy					1077:1082	significantly less creamy	1058:1082	significantly less creamy	1058:1082	Fore milk was significantly less creamy, less fatty, thinner, more watery, and lower in vanilla flavor intensity than hind milk.					
30594356	10	58	dep	way	1550:1552	arg1	develop					1626:1632	develop	1626:1632	develop healthier food preferences	1626:1659	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	8	59	theme	glutamic	1253:1260	arg1	content					1267:1273	glutamic acid content	1253:1273	glutamic acid content	1253:1273	Carbohydrate content of human milk was positively correlated with sweetness and glutamic acid content with umami.					
30594356	9	60	theme	hind	1420:1423	arg1	milk					1425:1428	hind milk	1420:1428	hind milk	1420:1428	The bitterness of the diet consumed 24 h before lactation was moderately positively correlated with bitterness of fore milk, but not hind milk.					
30594356	7	61	theme	hind	1162:1165	arg1	milk					1167:1170	hind milk	1162:1170	hind milk	1162:1170	Fore milk was significantly less creamy, less fatty, thinner, more watery, and lower in vanilla flavor intensity than hind milk.					
30594356	7	62	theme	fatty	1090:1094	arg1	creamy					1077:1082	significantly less creamy	1058:1082	significantly less creamy	1058:1082	Fore milk was significantly less creamy, less fatty, thinner, more watery, and lower in vanilla flavor intensity than hind milk.					
30594356	10	63	theme	for breastfed	1554:1566	arg1	children					1568:1575	for breastfed children	1554:1575	for breastfed children	1554:1575	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	1	64	theme	sensory	192:198	arg1	properties					200:209	sensory properties	192:209	sensory properties of fresh human milk	192:229	It is unknown how consumption of bitter foods and beverages in the maternal diet influences sensory properties of fresh human milk.					
30594356	4	65	theme	food	697:700	arg1	diary					702:706	a 24-h food diary	690:706	a 24-h food diary followed by a sensory self-assessment of their fore and hind milks	690:773	Mothers kept a 24-h food diary followed by a sensory self-assessment of their fore and hind milks.					
30594356	5	66	theme	neutral	822:828	arg1	creamy					831:836	creamy	831:836	creamy	831:836	The odor of human fresh milk was described as neutral, creamy, and sweet, taste as sweet and bitter, and mouthfeel as thin, watery, smooth, and fatty.					
30594356	5	66	theme	neutral	822:828	arg1	odor					780:783	The odor	776:783	The odor of human fresh milk	776:803	The odor of human fresh milk was described as neutral, creamy, and sweet, taste as sweet and bitter, and mouthfeel as thin, watery, smooth, and fatty.					
30594356	9	67	theme	diet	1309:1312	arg1	bitterness					1291:1300	The bitterness	1287:1300	The bitterness of the diet consumed 24 h before lactation	1287:1343	The bitterness of the diet consumed 24 h before lactation was moderately positively correlated with bitterness of fore milk, but not hind milk.					
30594356	2	68	theme	milk	429:432	arg1	properties					387:396	sensory properties	379:396	sensory properties	379:396	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	2	68	theme	milk	429:432	arg1	composition					402:412	composition	402:412	composition	402:412	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	5	69	theme	human	788:792	arg1	milk					800:803	human fresh milk	788:803	human fresh milk	788:803	The odor of human fresh milk was described as neutral, creamy, and sweet, taste as sweet and bitter, and mouthfeel as thin, watery, smooth, and fatty.					
30594356	0	70	dep	characteristics	8:22	arg1	Association					39:49	Association	39:49	Sensory characteristics of human milk: Association between mothers' diet and milk for bitter taste.	0:98	Sensory characteristics of human milk: Association between mothers' diet and milk for bitter taste.					
30594356	2	71	theme	human	314:318	arg1	fore					320:323	fresh human fore and hind milk	308:337	fore	320:323	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	7	72	theme	flavor	1140:1145	arg1	intensity					1147:1155	vanilla flavor intensity	1132:1155	vanilla flavor intensity	1132:1155	Fore milk was significantly less creamy, less fatty, thinner, more watery, and lower in vanilla flavor intensity than hind milk.					
30594356	2	73	theme	human	423:427	arg1	milk					429:432	fresh human milk	417:432	fresh human milk	417:432	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	3	74	theme	milk	611:614	arg1	perception					591:600	perception	591:600	perception of their milk	591:614	Twenty-two lactating mothers generated sensory terms to describe perception of their milk and received training on sensory attribute intensity rating.					
30594356	10	75	theme	human	1512:1516	arg1	milk					1523:1526	human fore milk	1512:1526	human fore milk	1512:1526	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	10	76	theme	bitter	1596:1601	arg1	vegetables					1603:1612	bitter vegetables	1596:1612	bitter vegetables	1596:1612	We conclude that the consumption of bitter foods may influence the bitterness of human fore milk, which may be another way for breastfed children to learn to accept bitter vegetables and, hence, develop healthier food preferences.					
30594356	2	77	theme	fresh	308:312	arg1	fore					320:323	fresh human fore and hind milk	308:337	fore	320:323	The aims of this study were (1) to determine the sensory characteristics of fresh human fore and hind milk, (2) to establish relationships between sensory properties and composition of fresh human milk, and (3) to assess the relationship between bitterness of the maternal diet and human milk.					
30594356	9	78	dep	h	1326:1326	arg1	lactation					1335:1343	lactation	1335:1343	lactation	1335:1343	The bitterness of the diet consumed 24 h before lactation was moderately positively correlated with bitterness of fore milk, but not hind milk.					
30594356	1	79	theme	fresh	214:218	arg1	milk					226:229	fresh human milk	214:229	fresh human milk	214:229	It is unknown how consumption of bitter foods and beverages in the maternal diet influences sensory properties of fresh human milk.					
29524853	10	0	theme	sugar	979:983	arg1	composition					985:995	sugar composition	979:995	sugar composition	979:995	The technique was applied to two species of Platanthera (Orchidaceae) in order to test whether species-related differences in sugar composition could be observed.					
29524853	5	1	contain	had	589:591	arg1	method					582:587	The analytical method	567:587	The analytical method	567:587	The analytical method had a linear range up to at least 3000 mg L-1 for all 3 sugars with a precision of 1.5-1.7%.					
29524853	5	1	contain	had	589:591	arg2	range					602:606	a linear range	593:606	a linear range up to at least 3000 mg L-1 for all 3 sugars	593:650	The analytical method had a linear range up to at least 3000 mg L-1 for all 3 sugars with a precision of 1.5-1.7%.					
29524853	5	2	theme	linear	595:600	arg1	range					602:606	a linear range	593:606	a linear range up to at least 3000 mg L-1 for all 3 sugars	593:650	The analytical method had a linear range up to at least 3000 mg L-1 for all 3 sugars with a precision of 1.5-1.7%.					
29524853	1	3	from	components	136:145	arg1	nectar					157:162	floral nectar	150:162	floral nectar	150:162	The dominant components in floral nectar is fructose, glucose and sucrose.					
29524853	3	4	attach	present	375:381	arg1	paper					366:370	this paper	361:370	this paper	361:370	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	3	4	attach	present	375:381	arg2	we					372:373	we	372:373	we	372:373	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	11	5	theme	individual	1161:1170	arg1	flowers					1172:1178	individual flowers	1161:1178	individual flowers	1161:1178	No differences were found between the two species, which were sucrose-dominant (53.5-100%) though with high variation within species and between individual flowers.					
29524853	11	6	dep	sucrose-dominant	1078:1093	arg1	sucrose-dominant					1078:1093	sucrose-dominant	1078:1093	sucrose-dominant	1078:1093	No differences were found between the two species, which were sucrose-dominant (53.5-100%) though with high variation within species and between individual flowers.					
29524853	11	6	dep	sucrose-dominant	1078:1093	arg1	%					1104:1104	53.5-100%	1096:1104	53.5-100%	1096:1104	No differences were found between the two species, which were sucrose-dominant (53.5-100%) though with high variation within species and between individual flowers.					
29524853	11	6	dep	sucrose-dominant	1078:1093	arg1	species					1058:1064	the two species	1050:1064	the two species	1050:1064	No differences were found between the two species, which were sucrose-dominant (53.5-100%) though with high variation within species and between individual flowers.					
29524853	1	7	theme	floral	150:155	arg1	nectar					157:162	floral nectar	150:162	floral nectar	150:162	The dominant components in floral nectar is fructose, glucose and sucrose.					
29524853	5	8	theme	analytical	571:580	arg1	method					582:587	The analytical method	567:587	The analytical method	567:587	The analytical method had a linear range up to at least 3000 mg L-1 for all 3 sugars with a precision of 1.5-1.7%.					
29524853	3	9	from	characterization	435:450	arg1	orchids					467:473	orchids	467:473	orchids	467:473	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	8	10	theme	Field	799:803	arg1	samples					805:811	Field samples	799:811	Field samples	799:811	Field samples were stable for min.					
29524853	3	11	theme	based	401:405	arg1	method					418:423	a novel HPLC-ELSD based analytical method	383:423	a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids	383:473	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	0	12	theme	novel	2:6	arg1	technique					8:16	A novel technique	0:16	A novel technique for determination of the fructose	0:50	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29524853	0	12	theme	novel	2:6	arg1	sucrose					65:71	sucrose	65:71	sucrose	65:71	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29524853	0	12	theme	novel	2:6	arg1	glucose					53:59	glucose	53:59	glucose	53:59	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29524853	10	13	from	differences	964:974	arg1	composition					985:995	sugar composition	979:995	sugar composition	979:995	The technique was applied to two species of Platanthera (Orchidaceae) in order to test whether species-related differences in sugar composition could be observed.					
29524853	7	14	theme	sugars	745:750	arg1	5-7 mg L-1					757:766	5-7 mg L-1	757:766	5-7 mg L-1	757:766	The LOD of all sugars were 5-7 mg L-1 and the LOQ were 17-19 mg L-1.					
29524853	7	14	theme	sugars	745:750	arg1	LOD					734:736	The LOD	730:736	The LOD of all sugars	730:750	The LOD of all sugars were 5-7 mg L-1 and the LOQ were 17-19 mg L-1.					
29524853	3	15	theme	novel	385:389	arg1	method					418:423	a novel HPLC-ELSD based analytical method	383:423	a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids	383:473	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	11	16	theme	high	1119:1122	arg1	variation					1124:1132	high variation	1119:1132	high variation within species and between individual flowers	1119:1178	No differences were found between the two species, which were sucrose-dominant (53.5-100%) though with high variation within species and between individual flowers.					
29524853	0	17	dep	glucose	53:59	arg1	distribution					73:84	distribution	73:84	distribution	73:84	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29524853	3	18	from	orchids	467:473	arg1	nectar					455:460	nectar	455:460	nectar from orchids	455:473	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	3	18	from	orchids	467:473	arg1	characterization					435:450	sugar characterization	429:450	sugar characterization of nectar from orchids	429:473	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	0	19	from	sucrose	65:71	arg1	nectar					89:94	nectar	89:94	nectar	89:94	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29524853	2	20	theme	plant	268:272	arg1	species					274:280	plant species	268:280	plant species	268:280	The concentration and the ratio between the sugars are indicative for plant species and play an important part in the interplay between plants and pollinators.					
29524853	3	21	theme	sugar	429:433	arg1	characterization					435:450	sugar characterization	429:450	sugar characterization of nectar from orchids	429:473	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	3	22	theme	analytical	407:416	arg1	method					418:423	a novel HPLC-ELSD based analytical method	383:423	a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids	383:473	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	3	23	theme	HPLC-ELSD	391:399	arg1	method					418:423	a novel HPLC-ELSD based analytical method	383:423	a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids	383:473	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	0	24	from	glucose	53:59	arg1	nectar					89:94	nectar	89:94	nectar	89:94	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29524853	6	25	theme	Correlation	682:692	arg1	coefficients					694:705	Correlation coefficients	682:705	Correlation coefficients	682:705	Correlation coefficients were 0.9999 to 1.0000.					
29524853	0	26	theme	fructose	43:50	arg1	determination					22:34	determination	22:34	determination of the fructose	22:50	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29524853	10	27	theme	Orchidaceae	910:920	arg1	species					886:892	two species	882:892	two species of Platanthera (Orchidaceae)	882:921	The technique was applied to two species of Platanthera (Orchidaceae) in order to test whether species-related differences in sugar composition could be observed.					
29524853	3	28	theme	nectar	455:460	arg1	characterization					435:450	sugar characterization	429:450	sugar characterization of nectar from orchids	429:473	In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids.					
29524853	11	29	with	sucrose-dominant	1078:1093	arg1	variation					1124:1132	high variation	1119:1132	high variation within species and between individual flowers	1119:1178	No differences were found between the two species, which were sucrose-dominant (53.5-100%) though with high variation within species and between individual flowers.					
29524853	4	30	theme	No.	508:510	arg1	paper					514:518	Whatman No. 1 paper	500:518	Whatman No. 1 paper	500:518	Nectar was collected on Whatman No. 1 paper and preserved in the field by 70 v/v% ethanol.					
29524853	10	31	theme	species-related	948:962	arg1	differences					964:974	species-related differences	948:974	species-related differences in sugar composition	948:995	The technique was applied to two species of Platanthera (Orchidaceae) in order to test whether species-related differences in sugar composition could be observed.					
29524853	4	32	theme	Whatman	500:506	arg1	paper					514:518	Whatman No. 1 paper	500:518	Whatman No. 1 paper	500:518	Nectar was collected on Whatman No. 1 paper and preserved in the field by 70 v/v% ethanol.					
29524853	2	33	theme	important	294:302	arg1	part					304:307	an important part	291:307	an important part	291:307	The concentration and the ratio between the sugars are indicative for plant species and play an important part in the interplay between plants and pollinators.					
29524853	4	34	theme	%	556:556	arg1	ethanol					558:564	70 v/v% ethanol	550:564	70 v/v% ethanol	550:564	Nectar was collected on Whatman No. 1 paper and preserved in the field by 70 v/v% ethanol.					
29524853	6	35	dep	1.0000	722:727	arg1	to					719:720	to	719:720	to	719:720	Correlation coefficients were 0.9999 to 1.0000.					
29524853	4	36	theme	70 v/v	550:555	arg1	%					556:556	%	556:556	%	556:556	Nectar was collected on Whatman No. 1 paper and preserved in the field by 70 v/v% ethanol.					
29524853	5	37	theme	%	679:679	arg1	precision					659:667	a precision	657:667	a precision of 1.5-1.7%	657:679	The analytical method had a linear range up to at least 3000 mg L-1 for all 3 sugars with a precision of 1.5-1.7%.					
29524853	1	38	theme	dominant	127:134	arg1	fructose					167:174	fructose	167:174	fructose	167:174	The dominant components in floral nectar is fructose, glucose and sucrose.					
29524853	1	38	theme	dominant	127:134	arg1	components					136:145	The dominant components	123:145	The dominant components in floral nectar	123:162	The dominant components in floral nectar is fructose, glucose and sucrose.					
29524853	0	39	from	orchids	101:107	arg1	technique					8:16	A novel technique	0:16	A novel technique for determination of the fructose	0:50	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29524853	0	39	from	orchids	101:107	arg1	sucrose					65:71	sucrose	65:71	sucrose	65:71	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29524853	0	39	from	orchids	101:107	arg1	glucose					53:59	glucose	53:59	glucose	53:59	A novel technique for determination of the fructose, glucose and sucrose distribution in nectar from orchids by HPLC-ELSD.					
29519324	3	0	theme	anti-inflammatory	503:519	arg1	action					521:526	anti-inflammatory action	503:526	anti-inflammatory action	503:526	Several species from the genus Croton have scientifically proven anti-inflammatory action.					
29519324	10	1	contain	had	1951:1953	arg2	effect					1970:1975	no significant effect	1955:1975	no significant effect	1955:1975	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	1	contain	had	1951:1953	arg1	β-caryophyllene					1935:1949	β-caryophyllene	1935:1949	β-caryophyllene	1935:1949	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	4	2	theme	Croton	596:601	arg1	EOCC					640:643	EOCC	640:643	EOCC	640:643	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	4	2	theme	Croton	596:601	arg1	oil					635:637	the Croton campestris A. St.-Hil essential oil	592:637	the Croton campestris A. St.-Hil essential oil (EOCC)	592:644	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	13	3	dep	suggesting	2526:2535	arg1	to					2523:2524	to	2523:2524	to	2523:2524	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	7	4	theme	granuloma	1371:1379	arg1	assays					1381:1386	granuloma assays	1371:1386	granuloma assays	1371:1386	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	12	5	contain	have	2199:2202	arg1	substances					2188:2197	The tested substances	2177:2197	The tested substances	2177:2197	CONCLUSION The tested substances have a clinically safe profile, additionally the EOCC and β-caryophyllene presented relevant anti-inflammatory activity.					
29519324	12	5	contain	have	2199:2202	arg2	profile					2222:2228	a clinically safe profile	2204:2228	a clinically safe profile	2204:2228	CONCLUSION The tested substances have a clinically safe profile, additionally the EOCC and β-caryophyllene presented relevant anti-inflammatory activity.					
29519324	10	6	theme	acid	1850:1853	arg1	test					1876:1879	the formalin test	1863:1879	the formalin test	1863:1879	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	6	theme	acid	1850:1853	arg1	test					1894:1897	peritonitis test	1882:1897	peritonitis test	1882:1897	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	6	theme	acid	1850:1853	arg1	permeability					1912:1923	vascular permeability	1903:1923	vascular permeability	1903:1923	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	6	theme	acid	1850:1853	arg1	models					1855:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	13	7	theme	acid	2660:2663	arg1	involvement					2629:2639	the involvement	2625:2639	the involvement of the arachidonic acid and histamine pathways	2625:2686	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	4	8	dep	EOCC	774:777	arg1	the					770:772	the	770:772	the	770:772	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	13	9	theme	anti-inflammatory	2476:2492	arg1	effect					2494:2499	the anti-inflammatory effect	2472:2499	the anti-inflammatory effect observed	2472:2508	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	6	10	theme	EOCC	946:949	arg1	composition					927:937	the chemical composition	914:937	the chemical composition of the EOCC	914:949	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	9	11	theme	relevant	1608:1615	arg1	toxicity					1626:1633	no relevant clinical toxicity	1605:1633	no relevant clinical toxicity	1605:1633	The EOCC had no relevant clinical toxicity on oral administration, with an LD50 of more than 5000 mg/kg.					
29519324	6	12	theme	Croton	1116:1121	arg1	oil					1123:1125	Croton oil	1116:1125	Croton oil	1116:1125	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	10	13	theme	formalin	1867:1874	arg1	test					1876:1879	the formalin test	1863:1879	the formalin test	1863:1879	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	13	theme	formalin	1867:1874	arg1	models					1855:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	4	14	dep	Croton	596:601	arg1	A.					614:615	Croton campestris A.	596:615	the Croton campestris A. St.-Hil essential oil (EOCC)	592:644	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	6	15	theme	effect	1062:1067	arg1	evaluation					1019:1028	the evaluation	1015:1028	the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil	1015:1125	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	7	16	theme	peritonitis	1327:1337	arg1	contortions					1204:1214	the abdominal contortions	1190:1214	the abdominal contortions	1190:1214	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	7	16	theme	peritonitis	1327:1337	arg1	test					1339:1342	a peritonitis test	1325:1342	a peritonitis test	1325:1342	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	10	17	theme	vascular	1903:1910	arg1	permeability					1912:1923	vascular permeability	1903:1923	vascular permeability	1903:1923	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	17	theme	vascular	1903:1910	arg1	models					1855:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	0	18	theme	β-caryophyllene	130:144	arg1	action					40:45	Anti-inflammatory and anti-edematogenic action	0:45	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.	0:163	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	11	19	theme	significant	2066:2076	arg1	influence					2078:2086	significant influence	2066:2086	significant influence on the arachidonic acid and histamine pathway reducing edema in these models	2066:2163	This suggests as a hypothesis that both substances tested showed significant influence on the arachidonic acid and histamine pathway reducing edema in these models.					
29519324	1	20	theme	vascularized	207:218	arg1	reactions					227:235	vascularized tissue reactions	207:235	vascularized tissue reactions acting in the defense of the body against harmful stimuli	207:293	BACKGROUND Inflammation makes up a set of vascularized tissue reactions acting in the defense of the body against harmful stimuli.					
29519324	13	21	with	association	2380:2390	arg1	constituents					2403:2414	other constituents	2397:2414	other constituents present in the EOCC such as 1,8-cineole	2397:2454	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	6	22	theme	chemical	918:925	arg1	composition					927:937	the chemical composition	914:937	the chemical composition of the EOCC	914:949	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	9	23	theme	oral	1638:1641	arg1	administration					1643:1656	oral administration	1638:1656	oral administration	1638:1656	The EOCC had no relevant clinical toxicity on oral administration, with an LD50 of more than 5000 mg/kg.					
29519324	0	24	theme	in	149:150	arg1	models					157:162	in vivo models	149:162	in vivo models	149:162	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	10	25	theme	tested	1701:1706	arg1	substances					1708:1717	The tested substances	1697:1717	The tested substances	1697:1717	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	5	26	theme	inflammation	889:900	arg1	models					861:866	in vivo models	853:866	in vivo models of acute and chronic inflammation	853:900	METHODS The assays were performed in in vivo models of acute and chronic inflammation.					
29519324	0	27	dep	in	149:150	arg1	vivo					152:155	vivo	152:155	vivo	152:155	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	13	28	from	EOCC	2431:2434	arg1	present					2416:2422	present	2416:2422	present	2416:2422	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	8	29	theme	essential	1434:1442	arg1	composition					1457:1467	the essential oil chemical composition	1430:1467	the essential oil chemical composition	1430:1467	RESULTS The evaluation of the essential oil chemical composition revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents.					
29519324	13	30	from	β-caryophyllene	2360:2374	arg1	association					2380:2390	association	2380:2390	association with other constituents present in the EOCC such as 1,8-cineole	2380:2454	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	0	31	theme	A.	72:73	arg1	oil					109:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	13	32	theme	mechanisms	2553:2562	arg1	one					2542:2544	one	2542:2544	one	2542:2544	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	13	32	theme	mechanisms	2553:2562	arg1	mechanisms					2553:2562	the mechanisms	2549:2562	the mechanisms of action	2549:2572	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	11	33	theme	pathway	2126:2132	arg1	edema					2143:2147	histamine pathway reducing edema	2116:2147	histamine pathway reducing edema	2116:2147	This suggests as a hypothesis that both substances tested showed significant influence on the arachidonic acid and histamine pathway reducing edema in these models.					
29519324	8	34	theme	chemical	1448:1455	arg1	composition					1457:1467	the essential oil chemical composition	1430:1467	the essential oil chemical composition	1430:1467	RESULTS The evaluation of the essential oil chemical composition revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents.					
29519324	10	35	theme	significant	1958:1968	arg1	effect					1970:1975	no significant effect	1955:1975	no significant effect	1955:1975	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	13	36	theme	cytokines	2610:2618	arg1	inhibition					2596:2605	the inhibition	2592:2605	the inhibition of cytokines	2592:2618	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	12	37	theme	safe	2217:2220	arg1	profile					2222:2228	a clinically safe profile	2204:2228	a clinically safe profile	2204:2228	CONCLUSION The tested substances have a clinically safe profile, additionally the EOCC and β-caryophyllene presented relevant anti-inflammatory activity.					
29519324	7	38	theme	action	1182:1187	arg1	verification					1145:1156	the systemic verification	1132:1156	the systemic verification of an anti-inflammatory action	1132:1187	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	4	39	theme	action	760:765	arg1	mechanisms					746:755	the possible mechanisms	733:755	the possible mechanisms of action of the EOCC and its constituent β-caryophyllene	733:813	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	4	39	theme	action	760:765	arg1	potential					719:727	the anti-inflammatory potential	697:727	the anti-inflammatory potential	697:727	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	2	40	theme	popular	377:383	arg1	medicine					385:392	popular medicine	377:392	popular medicine	377:392	Natural products are a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes.					
29519324	0	41	theme	oil	109:111	arg1	action					40:45	Anti-inflammatory and anti-edematogenic action	0:45	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.	0:163	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	7	42	theme	acid	1301:1304	arg1	models					1306:1311	arachidonic acid models	1289:1311	arachidonic acid models	1289:1311	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	8	43	theme	β-caryophyllene	1494:1508	arg1	presence					1482:1489	the presence	1478:1489	the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents	1478:1589	RESULTS The evaluation of the essential oil chemical composition revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents.					
29519324	0	44	theme	St.-Hil	75:81	arg1	oil					109:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	0	45	theme	Anti-inflammatory	0:16	arg1	action					40:45	Anti-inflammatory and anti-edematogenic action	0:45	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.	0:163	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	1	46	theme	body	266:269	arg1	defense					251:257	the defense	247:257	the defense of the body against harmful stimuli	247:293	BACKGROUND Inflammation makes up a set of vascularized tissue reactions acting in the defense of the body against harmful stimuli.					
29519324	10	47	theme	paw	1781:1783	arg1	edema					1785:1789	paw edema	1781:1789	paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability	1781:1923	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	2	48	theme	inflammatory	414:425	arg1	processes					427:435	inflammatory processes	414:435	inflammatory processes	414:435	Natural products are a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes.					
29519324	4	49	theme	constituent	787:797	arg1	β-caryophyllene					799:813	its constituent β-caryophyllene	783:813	its constituent β-caryophyllene	783:813	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	0	50	theme	anti-edematogenic	22:38	arg1	action					40:45	Anti-inflammatory and anti-edematogenic action	0:45	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.	0:163	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	1	51	theme	harmful	279:285	arg1	stimuli					287:293	harmful stimuli	279:293	harmful stimuli	279:293	BACKGROUND Inflammation makes up a set of vascularized tissue reactions acting in the defense of the body against harmful stimuli.					
29519324	4	52	attach	derived	647:653	arg2	oil					635:637	the Croton campestris A. St.-Hil essential oil	592:637	the Croton campestris A. St.-Hil essential oil (EOCC)	592:644	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	4	52	attach	derived	647:653	arg2	EOCC					640:643	EOCC	640:643	EOCC	640:643	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	4	52	attach	derived	647:653	arg1	fresh					660:664	fresh	660:664	fresh	660:664	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	5	53	dep	in	853:854	arg1	vivo					856:859	vivo	856:859	vivo	856:859	METHODS The assays were performed in in vivo models of acute and chronic inflammation.					
29519324	3	54	theme	Several	438:444	arg1	species					446:452	Several species	438:452	Several species from the genus Croton	438:474	Several species from the genus Croton have scientifically proven anti-inflammatory action.					
29519324	12	55	dep	profile	2222:2228	arg1	presented					2273:2281	presented	2273:2281	presented relevant anti-inflammatory activity	2273:2317	CONCLUSION The tested substances have a clinically safe profile, additionally the EOCC and β-caryophyllene presented relevant anti-inflammatory activity.					
29519324	11	56	theme	histamine	2116:2124	arg1	edema					2143:2147	histamine pathway reducing edema	2116:2147	histamine pathway reducing edema	2116:2147	This suggests as a hypothesis that both substances tested showed significant influence on the arachidonic acid and histamine pathway reducing edema in these models.					
29519324	2	57	with	alternative	330:340	arg1	benefit					354:360	better benefit	347:360	better benefit	347:360	Natural products are a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes.					
29519324	11	58	theme	arachidonic	2095:2105	arg1	acid					2107:2110	the arachidonic acid	2091:2110	the arachidonic acid	2091:2110	This suggests as a hypothesis that both substances tested showed significant influence on the arachidonic acid and histamine pathway reducing edema in these models.					
29519324	8	59	theme	1,8-cineol	1520:1529	arg1	presence					1482:1489	the presence	1478:1489	the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents	1478:1589	RESULTS The evaluation of the essential oil chemical composition revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents.					
29519324	0	60	theme	Croton	54:59	arg1	oil					109:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	8	61	dep	RESULTS	1404:1410	arg1	revealed					1469:1476	revealed	1469:1476	revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents	1469:1589	RESULTS The evaluation of the essential oil chemical composition revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents.					
29519324	0	62	from	action	40:45	arg1	models					157:162	in vivo models	149:162	in vivo models	149:162	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	10	63	theme	granuloma	1984:1992	arg1	assay					1994:1998	the granuloma assay	1980:1998	the granuloma assay	1980:1998	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	64	theme	carrageenan	1802:1812	arg1	test					1876:1879	the formalin test	1863:1879	the formalin test	1863:1879	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	64	theme	carrageenan	1802:1812	arg1	test					1894:1897	peritonitis test	1882:1897	peritonitis test	1882:1897	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	64	theme	carrageenan	1802:1812	arg1	permeability					1912:1923	vascular permeability	1903:1923	vascular permeability	1903:1923	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	64	theme	carrageenan	1802:1812	arg1	models					1855:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	3	65	theme	genus	463:467	arg1	Croton					469:474	the genus Croton	459:474	the genus Croton	459:474	Several species from the genus Croton have scientifically proven anti-inflammatory action.					
29519324	1	66	theme	tissue	220:225	arg1	reactions					227:235	vascularized tissue reactions	207:235	vascularized tissue reactions acting in the defense of the body against harmful stimuli	207:293	BACKGROUND Inflammation makes up a set of vascularized tissue reactions acting in the defense of the body against harmful stimuli.					
29519324	6	67	theme	chronic	1087:1093	arg1	edema					1099:1103	acute and chronic ear edema	1077:1103	acute and chronic ear edema induced by Croton oil	1077:1125	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	5	68	dep	METHODS	816:822	arg1	assays					828:833	The assays	824:833	METHODS The assays	816:833	METHODS The assays were performed in in vivo models of acute and chronic inflammation.					
29519324	6	69	theme	acute	1077:1081	arg1	edema					1099:1103	acute and chronic ear edema	1077:1103	acute and chronic ear edema induced by Croton oil	1077:1125	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	8	70	theme	germacrene-D	1544:1555	arg1	presence					1482:1489	the presence	1478:1489	the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents	1478:1589	RESULTS The evaluation of the essential oil chemical composition revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents.					
29519324	10	71	theme	histamine	1824:1832	arg1	test					1876:1879	the formalin test	1863:1879	the formalin test	1863:1879	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	71	theme	histamine	1824:1832	arg1	test					1894:1897	peritonitis test	1882:1897	peritonitis test	1882:1897	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	71	theme	histamine	1824:1832	arg1	permeability					1912:1923	vascular permeability	1903:1923	vascular permeability	1903:1923	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	71	theme	histamine	1824:1832	arg1	models					1855:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	2	72	theme	better	347:352	arg1	benefit					354:360	better benefit	347:360	better benefit	347:360	Natural products are a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes.					
29519324	13	73	theme	pathways	2679:2686	arg1	involvement					2629:2639	the involvement	2625:2639	the involvement of the arachidonic acid and histamine pathways	2625:2686	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	7	74	theme	formalin	1217:1224	arg1	test					1226:1229	formalin test	1217:1229	formalin test	1217:1229	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	7	74	theme	formalin	1217:1224	arg1	contortions					1204:1214	the abdominal contortions	1190:1214	the abdominal contortions	1190:1214	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	12	75	theme	anti-inflammatory	2292:2308	arg1	activity					2310:2317	relevant anti-inflammatory activity	2283:2317	relevant anti-inflammatory activity	2283:2317	CONCLUSION The tested substances have a clinically safe profile, additionally the EOCC and β-caryophyllene presented relevant anti-inflammatory activity.					
29519324	10	76	theme	dextran	1815:1821	arg1	test					1876:1879	the formalin test	1863:1879	the formalin test	1863:1879	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	76	theme	dextran	1815:1821	arg1	test					1894:1897	peritonitis test	1882:1897	peritonitis test	1882:1897	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	76	theme	dextran	1815:1821	arg1	permeability					1912:1923	vascular permeability	1903:1923	vascular permeability	1903:1923	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	76	theme	dextran	1815:1821	arg1	models					1855:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	11	77	from	influence	2078:2086	arg1	edema					2143:2147	histamine pathway reducing edema	2116:2147	histamine pathway reducing edema	2116:2147	This suggests as a hypothesis that both substances tested showed significant influence on the arachidonic acid and histamine pathway reducing edema in these models.					
29519324	11	77	from	influence	2078:2086	arg1	models					2158:2163	these models	2152:2163	these models	2152:2163	This suggests as a hypothesis that both substances tested showed significant influence on the arachidonic acid and histamine pathway reducing edema in these models.					
29519324	11	77	from	influence	2078:2086	arg1	acid					2107:2110	the arachidonic acid	2091:2110	the arachidonic acid	2091:2110	This suggests as a hypothesis that both substances tested showed significant influence on the arachidonic acid and histamine pathway reducing edema in these models.					
29519324	13	78	theme	arachidonic	2648:2658	arg1	acid					2660:2663	the arachidonic acid	2644:2663	the arachidonic acid	2644:2663	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	1	79	theme	BACKGROUND	165:174	arg1	Inflammation					176:187	BACKGROUND Inflammation	165:187	BACKGROUND Inflammation	165:187	BACKGROUND Inflammation makes up a set of vascularized tissue reactions acting in the defense of the body against harmful stimuli.					
29519324	10	80	theme	arachidonic	1838:1848	arg1	acid					1850:1853	arachidonic acid	1838:1853	arachidonic acid	1838:1853	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	3	81	from	Croton	469:474	arg1	species					446:452	Several species	438:452	Several species from the genus Croton	438:474	Several species from the genus Croton have scientifically proven anti-inflammatory action.					
29519324	5	82	theme	in	853:854	arg1	models					861:866	in vivo models	853:866	in vivo models of acute and chronic inflammation	853:900	METHODS The assays were performed in in vivo models of acute and chronic inflammation.					
29519324	0	83	theme	compound	121:128	arg1	β-caryophyllene					130:144	the compound β-caryophyllene	117:144	the compound β-caryophyllene	117:144	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	2	84	theme	Natural	296:302	arg1	alternative					330:340	a lower cost alternative	317:340	a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes	317:435	Natural products are a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes.					
29519324	2	84	theme	Natural	296:302	arg1	products					304:311	Natural products	296:311	Natural products	296:311	Natural products are a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes.					
29519324	9	85	theme	clinical	1617:1624	arg1	toxicity					1626:1633	no relevant clinical toxicity	1605:1633	no relevant clinical toxicity	1605:1633	The EOCC had no relevant clinical toxicity on oral administration, with an LD50 of more than 5000 mg/kg.					
29519324	7	86	theme	vascular	1345:1352	arg1	permeability					1354:1365	vascular permeability	1345:1365	vascular permeability	1345:1365	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	4	87	theme	oil	635:637	arg1	composition					577:587	the chemical composition	564:587	the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves,	564:672	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	11	88	theme	reducing	2134:2141	arg1	edema					2143:2147	histamine pathway reducing edema	2116:2147	histamine pathway reducing edema	2116:2147	This suggests as a hypothesis that both substances tested showed significant influence on the arachidonic acid and histamine pathway reducing edema in these models.					
29519324	10	89	theme	anti-inflammatory	1726:1742	arg1	action					1744:1749	anti-inflammatory action	1726:1749	anti-inflammatory action	1726:1749	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	12	90	theme	relevant	2283:2290	arg1	activity					2310:2317	relevant anti-inflammatory activity	2283:2317	relevant anti-inflammatory activity	2283:2317	CONCLUSION The tested substances have a clinically safe profile, additionally the EOCC and β-caryophyllene presented relevant anti-inflammatory activity.					
29519324	7	91	theme	anti-inflammatory	1164:1180	arg1	action					1182:1187	an anti-inflammatory action	1161:1187	an anti-inflammatory action	1161:1187	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	13	92	theme	present	2416:2422	arg1	constituents					2403:2414	other constituents	2397:2414	other constituents present in the EOCC such as 1,8-cineole	2397:2454	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	4	93	theme	St.-Hil	617:623	arg1	EOCC					640:643	EOCC	640:643	EOCC	640:643	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	4	93	theme	St.-Hil	617:623	arg1	oil					635:637	the Croton campestris A. St.-Hil essential oil	592:637	the Croton campestris A. St.-Hil essential oil (EOCC)	592:644	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	5	94	theme	chronic	881:887	arg1	inflammation					889:900	acute and chronic inflammation	871:900	acute and chronic inflammation	871:900	METHODS The assays were performed in in vivo models of acute and chronic inflammation.					
29519324	10	95	theme	peritonitis	1882:1892	arg1	test					1894:1897	peritonitis test	1882:1897	peritonitis test	1882:1897	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	95	theme	peritonitis	1882:1892	arg1	models					1855:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	carrageenan, dextran, histamine and arachidonic acid models	1802:1860	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	10	96	theme	abdominal	1758:1766	arg1	contortions					1768:1778	the abdominal contortions	1754:1778	the abdominal contortions	1754:1778	The tested substances showed anti-inflammatory action in the abdominal contortions, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, the formalin test, peritonitis test and vascular permeability; however, β-caryophyllene had no significant effect on the granuloma assay.					
29519324	8	97	theme	oil	1444:1446	arg1	composition					1457:1467	the essential oil chemical composition	1430:1467	the essential oil chemical composition	1430:1467	RESULTS The evaluation of the essential oil chemical composition revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents.					
29519324	6	98	theme	ear	1095:1097	arg1	edema					1099:1103	acute and chronic ear edema	1077:1103	acute and chronic ear edema induced by Croton oil	1077:1125	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	7	99	theme	abdominal	1194:1202	arg1	test					1226:1229	formalin test	1217:1229	formalin test	1217:1229	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	7	99	theme	abdominal	1194:1202	arg1	edema					1236:1240	paw edema	1232:1240	paw edema induced by carrageenan, dextran, histamine and arachidonic acid models	1232:1311	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	7	99	theme	abdominal	1194:1202	arg1	test					1339:1342	a peritonitis test	1325:1342	a peritonitis test	1325:1342	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	7	99	theme	abdominal	1194:1202	arg1	contortions					1204:1214	the abdominal contortions	1190:1214	the abdominal contortions	1190:1214	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	12	100	dep	CONCLUSION	2166:2175	arg1	have					2199:2202	have	2199:2202	have a clinically safe profile, additionally the EOCC and β-caryophyllene presented relevant anti-inflammatory activity	2199:2317	CONCLUSION The tested substances have a clinically safe profile, additionally the EOCC and β-caryophyllene presented relevant anti-inflammatory activity.					
29519324	13	101	from	present	2416:2422	arg1	EOCC					2431:2434	the EOCC	2427:2434	the EOCC such as 1,8-cineole	2427:2454	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	8	102	theme	composition	1457:1467	arg1	evaluation					1416:1425	The evaluation	1412:1425	The evaluation of the essential oil chemical composition	1412:1467	RESULTS The evaluation of the essential oil chemical composition revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents.					
29519324	13	103	theme	action	2567:2572	arg1	mechanisms					2553:2562	the mechanisms	2549:2562	the mechanisms of action	2549:2572	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	0	104	theme	Euphorbiaceae	84:96	arg1	oil					109:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	5	105	theme	acute	871:875	arg1	inflammation					889:900	acute and chronic inflammation	871:900	acute and chronic inflammation	871:900	METHODS The assays were performed in in vivo models of acute and chronic inflammation.					
29519324	6	106	theme	oral	974:977	arg1	toxicity					979:986	its oral toxicity	970:986	its oral toxicity	970:986	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	9	107	theme	more	1675:1678	arg1	LD50					1667:1670	an LD50	1664:1670	an LD50 of more than 5000 mg/kg	1664:1694	The EOCC had no relevant clinical toxicity on oral administration, with an LD50 of more than 5000 mg/kg.					
29519324	0	108	theme	essential	99:107	arg1	oil					109:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	4	109	dep	PURPOSE	529:535	arg1	aims					548:551	aims	548:551	aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene	548:813	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	12	110	theme	tested	2181:2186	arg1	substances					2188:2197	The tested substances	2177:2197	The tested substances	2177:2197	CONCLUSION The tested substances have a clinically safe profile, additionally the EOCC and β-caryophyllene presented relevant anti-inflammatory activity.					
29519324	4	111	theme	possible	737:744	arg1	mechanisms					746:755	the possible mechanisms	733:755	the possible mechanisms of action of the EOCC and its constituent β-caryophyllene	733:813	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	13	112	theme	other	2397:2401	arg1	constituents					2403:2414	other constituents	2397:2414	other constituents present in the EOCC such as 1,8-cineole	2397:2454	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	6	113	theme	antiedematogenic	1045:1060	arg1	effect					1062:1067	its topical antiedematogenic effect	1033:1067	its topical antiedematogenic effect	1033:1067	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	7	114	theme	systemic	1136:1143	arg1	verification					1145:1156	the systemic verification	1132:1156	the systemic verification of an anti-inflammatory action	1132:1187	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	2	115	theme	processes	427:435	arg1	treatment					401:409	the treatment	397:409	the treatment of inflammatory processes	397:435	Natural products are a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes.					
29519324	4	116	theme	anti-inflammatory	701:717	arg1	potential					719:727	the anti-inflammatory potential	697:727	the anti-inflammatory potential	697:727	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	4	117	theme	chemical	568:575	arg1	composition					577:587	the chemical composition	564:587	the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves,	564:672	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	9	118	contain	had	1601:1603	arg1	EOCC					1596:1599	The EOCC	1592:1599	The EOCC	1592:1599	The EOCC had no relevant clinical toxicity on oral administration, with an LD50 of more than 5000 mg/kg.					
29519324	9	118	contain	had	1601:1603	arg2	toxicity					1626:1633	no relevant clinical toxicity	1605:1633	no relevant clinical toxicity	1605:1633	The EOCC had no relevant clinical toxicity on oral administration, with an LD50 of more than 5000 mg/kg.					
29519324	7	119	theme	arachidonic	1289:1299	arg1	models					1306:1311	arachidonic acid models	1289:1311	arachidonic acid models	1289:1311	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	0	120	theme	campestris	61:70	arg1	oil					109:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil	50:111	Anti-inflammatory and anti-edematogenic action of the Croton campestris A. St.-Hil (Euphorbiaceae) essential oil and the compound β-caryophyllene in in vivo models.					
29519324	4	121	theme	β-caryophyllene	799:813	arg1	action					760:765	action	760:765	action of the EOCC and its constituent β-caryophyllene	760:813	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	2	122	theme	cost	325:328	arg1	alternative					330:340	a lower cost alternative	317:340	a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes	317:435	Natural products are a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes.					
29519324	2	122	theme	cost	325:328	arg1	products					304:311	Natural products	296:311	Natural products	296:311	Natural products are a lower cost alternative with better benefit, often used in popular medicine in the treatment of inflammatory processes.					
29519324	8	123	theme	main	1573:1576	arg1	constituents					1578:1589	its main constituents	1569:1589	its main constituents	1569:1589	RESULTS The evaluation of the essential oil chemical composition revealed the presence of β-caryophyllene (15.91%), 1,8-cineol (16.98%) and germacrene-D (14.51%) as its main constituents.					
29519324	4	124	dep	fresh	660:664	arg1	leaves					666:671	leaves	666:671	leaves	666:671	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	7	125	theme	paw	1232:1234	arg1	edema					1236:1240	paw edema	1232:1240	paw edema induced by carrageenan, dextran, histamine and arachidonic acid models	1232:1311	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	7	125	theme	paw	1232:1234	arg1	contortions					1204:1214	the abdominal contortions	1190:1214	the abdominal contortions	1190:1214	For the systemic verification of an anti-inflammatory action, the abdominal contortions, formalin test, paw edema induced by carrageenan, dextran, histamine and arachidonic acid models, as well as a peritonitis test, vascular permeability and granuloma assays were performed.					
29519324	13	126	attach	present	2416:2422	arg2	constituents					2403:2414	other constituents	2397:2414	other constituents present in the EOCC such as 1,8-cineole	2397:2454	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	13	126	attach	present	2416:2422	arg1	EOCC					2431:2434	the EOCC	2427:2434	the EOCC such as 1,8-cineole	2427:2454	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
29519324	1	127	theme	reactions	227:235	arg1	set					200:202	a set	198:202	a set of vascularized tissue reactions acting in the defense of the body against harmful stimuli	198:293	BACKGROUND Inflammation makes up a set of vascularized tissue reactions acting in the defense of the body against harmful stimuli.					
29519324	1	127	theme	reactions	227:235	arg1	reactions					227:235	vascularized tissue reactions	207:235	vascularized tissue reactions acting in the defense of the body against harmful stimuli	207:293	BACKGROUND Inflammation makes up a set of vascularized tissue reactions acting in the defense of the body against harmful stimuli.					
29519324	6	128	theme	topical	1037:1043	arg1	effect					1062:1067	its topical antiedematogenic effect	1033:1067	its topical antiedematogenic effect	1033:1067	Initially, the chemical composition of the EOCC was determined and its oral toxicity was evaluated, followed by the evaluation of its topical antiedematogenic effect through acute and chronic ear edema induced by Croton oil.					
29519324	4	129	theme	essential	625:633	arg1	EOCC					640:643	EOCC	640:643	EOCC	640:643	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	4	129	theme	essential	625:633	arg1	oil					635:637	the Croton campestris A. St.-Hil essential oil	592:637	the Croton campestris A. St.-Hil essential oil (EOCC)	592:644	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	4	130	theme	EOCC	774:777	arg1	action					760:765	action	760:765	action of the EOCC and its constituent β-caryophyllene	760:813	PURPOSE This study aims to analyze the chemical composition of the Croton campestris A. St.-Hil essential oil (EOCC), derived from fresh leaves, as well as to evaluate the anti-inflammatory potential and the possible mechanisms of action of the EOCC and its constituent β-caryophyllene.					
29519324	13	131	theme	histamine	2669:2677	arg1	pathways					2679:2686	histamine pathways	2669:2686	histamine pathways	2669:2686	This study supports the hypothesis that β-caryophyllene, in association with other constituents present in the EOCC such as 1,8-cineole, contributed to the anti-inflammatory effect observed, in addition to suggesting that one of the mechanisms of action probably involves the inhibition of cytokines with the involvement of the arachidonic acid and histamine pathways.					
30413947	3	0	theme	term	352:355	arg1	failure					357:363	long term failure	347:363	long term failure due to calcification	347:384	Still they suffer from long term failure due to calcification.					
30413947	8	1	dep	tissues	1124:1130	arg1	b					1104:1104	b	1104:1104	b	1104:1104	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	5	2	theme	anti-inflammatory	586:602	arg1	activity					604:611	anti-inflammatory activity	586:611	anti-inflammatory activity	586:611	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	7	3	theme	constant	971:978	arg1	model					992:996	the constant composition model	967:996	the constant composition model	967:996	After that, heparin hydrogel embedded bovine pericardial tissues, fixed with glutaraldehyde, were produced and tested for their mechanical behavior and anticalcifcation potential in vitro using the constant composition model.					
30413947	0	4	theme	pericardial	72:82	arg1	tissues					84:90	glutaraldehyde fixed bovine pericardial tissues	44:90	glutaraldehyde fixed bovine pericardial tissues	44:90	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	0	5	from	effect	4:9	arg1	tissues					84:90	glutaraldehyde fixed bovine pericardial tissues	44:90	glutaraldehyde fixed bovine pericardial tissues	44:90	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	5	6	theme	calcification	653:665	arg1	problem					667:673	calcification problem	653:673	calcification problem	653:673	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	12	7	theme	Histological	1613:1624	arg1	data					1626:1629	Histological data	1613:1629	Histological data	1613:1629	Histological data confirmed the obtained results, suggesting that heparin treatment is a promising anticalcification agent.					
30413947	10	8	theme	Calcification	1316:1328	arg1	assessment					1330:1339	Calcification assessment	1316:1339	Calcification assessment	1316:1339	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	7	9	theme	embedded	802:809	arg1	tissues					830:836	heparin hydrogel embedded bovine pericardial tissues	785:836	heparin hydrogel embedded bovine pericardial tissues	785:836	After that, heparin hydrogel embedded bovine pericardial tissues, fixed with glutaraldehyde, were produced and tested for their mechanical behavior and anticalcifcation potential in vitro using the constant composition model.					
30413947	0	10	theme	Mechanical	93:102	arg1	behavior					104:111	Mechanical behavior	93:111	Mechanical behavior	93:111	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	8	11	theme	Hydrogel	1107:1114	arg1	tissues					1124:1130	Hydrogel treated tissues	1107:1130	Hydrogel treated tissues	1107:1130	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	8	11	theme	Hydrogel	1107:1114	arg1	groups					1065:1070	three groups	1059:1070	three groups	1059:1070	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	1	12	from	common	174:179	arg1	countries					199:207	industrialized countries	184:207	industrialized countries	184:207	Heart valve diseases remain common in industrialized countries.					
30413947	10	13	theme	calcium	1388:1394	arg1	deposition					1406:1415	calcium phosphate deposition	1388:1415	calcium phosphate deposition of hydrogel treated (second group)	1388:1450	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	10	14	theme	first	1497:1501	arg1	group					1503:1507	first group	1497:1507	first group	1497:1507	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	8	15	dep	groups	1065:1070	arg1	Controls					1076:1083	Controls	1076:1083	a) Controls without treatment	1073:1101	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	9	16	contain	had	1263:1265	arg2	effect					1281:1286	no stiffening effect	1267:1286	no stiffening effect	1267:1286	The results showed that embedding of tissue with hydrogel had no stiffening effect on its mechanical behavior.					
30413947	9	16	contain	had	1263:1265	arg1	embedding					1229:1237	embedding	1229:1237	embedding of tissue with hydrogel	1229:1261	The results showed that embedding of tissue with hydrogel had no stiffening effect on its mechanical behavior.					
30413947	11	17	theme	raw	1586:1588	arg1	heparin					1590:1596	raw heparin	1586:1596	raw heparin (third group)	1586:1610	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	11	17	theme	raw	1586:1588	arg1	group					1605:1609	third group	1599:1609	third group	1599:1609	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	5	18	theme	high	544:547	arg1	charge					558:563	high negative charge	544:563	high negative charge	544:563	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	8	19	theme	treated	1116:1122	arg1	tissues					1124:1130	Hydrogel treated tissues	1107:1130	Hydrogel treated tissues	1107:1130	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	8	19	theme	treated	1116:1122	arg1	groups					1065:1070	three groups	1059:1070	three groups	1059:1070	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	9	20	with	embedding	1229:1237	arg1	hydrogel					1254:1261	hydrogel	1254:1261	hydrogel	1254:1261	The results showed that embedding of tissue with hydrogel had no stiffening effect on its mechanical behavior.					
30413947	5	21	theme	negative	549:556	arg1	charge					558:563	high negative charge	544:563	high negative charge	544:563	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	1	22	theme	industrialized	184:197	arg1	countries					199:207	industrialized countries	184:207	industrialized countries	184:207	Heart valve diseases remain common in industrialized countries.					
30413947	11	23	from	second	1575:1580	arg1	similar					1555:1561	similar	1555:1561	similar	1555:1561	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	11	24	theme	Calcification	1511:1523	arg1	potential					1536:1544	Calcification inhibition potential	1511:1544	Calcification inhibition potential	1511:1544	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	5	25	theme	potential	630:638	arg1	solution					640:647	a potential solution	628:647	a potential solution for calcification problem	628:673	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	7	26	theme	anticalcifcation	925:940	arg1	potential					942:950	anticalcifcation potential	925:950	anticalcifcation potential	925:950	After that, heparin hydrogel embedded bovine pericardial tissues, fixed with glutaraldehyde, were produced and tested for their mechanical behavior and anticalcifcation potential in vitro using the constant composition model.					
30413947	11	27	theme	third	1599:1603	arg1	heparin					1590:1596	raw heparin	1586:1596	raw heparin (third group)	1586:1610	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	11	27	theme	third	1599:1603	arg1	group					1605:1609	third group	1599:1609	third group	1599:1609	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	9	28	theme	stiffening	1270:1279	arg1	effect					1281:1286	no stiffening effect	1267:1286	no stiffening effect	1267:1286	The results showed that embedding of tissue with hydrogel had no stiffening effect on its mechanical behavior.					
30413947	5	29	contain	possess	515:521	arg2	properties					523:532	properties	523:532	properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem	523:673	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	5	29	contain	possess	515:521	arg2	anticoagulation					566:580	anticoagulation	566:580	anticoagulation	566:580	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	5	29	contain	possess	515:521	arg1	GAGs					509:512	GAGs	509:512	GAGs	509:512	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	5	29	contain	possess	515:521	arg2	charge					558:563	high negative charge	544:563	high negative charge	544:563	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	5	29	contain	possess	515:521	arg2	activity					604:611	anti-inflammatory activity	586:611	anti-inflammatory activity	586:611	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	5	29	contain	possess	515:521	arg1	Glycosaminoglycans					489:506	Glycosaminoglycans	489:506	Glycosaminoglycans (GAGs)	489:513	Glycosaminoglycans (GAGs) possess properties including high negative charge, anticoagulation and anti-inflammatory activity that make them a potential solution for calcification problem.					
30413947	7	30	theme	mechanical	901:910	arg1	behavior					912:919	their mechanical behavior	895:919	their mechanical behavior	895:919	After that, heparin hydrogel embedded bovine pericardial tissues, fixed with glutaraldehyde, were produced and tested for their mechanical behavior and anticalcifcation potential in vitro using the constant composition model.					
30413947	0	31	theme	heparin	14:20	arg1	embedding					31:39	heparin hydrogel embedding	14:39	heparin hydrogel embedding	14:39	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	0	32	dep	effect	4:9	arg1	behavior					104:111	Mechanical behavior	93:111	Mechanical behavior	93:111	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	0	32	dep	effect	4:9	arg1	potential					135:143	anticalcification potential	117:143	anticalcification potential	117:143	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	9	33	theme	tissue	1242:1247	arg1	embedding					1229:1237	embedding	1229:1237	embedding of tissue with hydrogel	1229:1261	The results showed that embedding of tissue with hydrogel had no stiffening effect on its mechanical behavior.					
30413947	7	34	with	fixed	839:843	arg1	glutaraldehyde					850:863	glutaraldehyde	850:863	glutaraldehyde	850:863	After that, heparin hydrogel embedded bovine pericardial tissues, fixed with glutaraldehyde, were produced and tested for their mechanical behavior and anticalcifcation potential in vitro using the constant composition model.					
30413947	11	35	from	similar	1555:1561	arg1	second					1575:1580	second	1575:1580	second	1575:1580	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	11	35	from	similar	1555:1561	arg1	heparin					1590:1596	raw heparin	1586:1596	raw heparin (third group)	1586:1610	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	11	35	from	similar	1555:1561	arg1	group					1605:1609	third group	1599:1609	third group	1599:1609	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	12	36	theme	heparin	1679:1685	arg1	agent					1730:1734	a promising anticalcification agent	1700:1734	a promising anticalcification agent	1700:1734	Histological data confirmed the obtained results, suggesting that heparin treatment is a promising anticalcification agent.					
30413947	12	36	theme	heparin	1679:1685	arg1	treatment					1687:1695	heparin treatment	1679:1695	heparin treatment	1679:1695	Histological data confirmed the obtained results, suggesting that heparin treatment is a promising anticalcification agent.					
30413947	0	37	theme	anticalcification	117:133	arg1	potential					135:143	anticalcification potential	117:143	anticalcification potential	117:143	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	10	38	theme	untreated	1469:1477	arg1	control					1488:1494	control	1488:1494	control	1488:1494	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	10	38	theme	untreated	1469:1477	arg1	tissues					1479:1485	untreated tissues	1469:1485	untreated tissues (control, first group)	1469:1508	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	11	39	theme	inhibition	1525:1534	arg1	potential					1536:1544	Calcification inhibition potential	1511:1544	Calcification inhibition potential	1511:1544	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	8	40	with	tissues	1124:1130	arg1	heparin					1156:1162	raw heparin	1152:1162	raw heparin	1152:1162	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	10	41	theme	phosphate	1396:1404	arg1	deposition					1406:1415	calcium phosphate deposition	1388:1415	calcium phosphate deposition of hydrogel treated (second group)	1388:1450	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	0	42	theme	embedding	31:39	arg1	effect					4:9	The effect	0:9	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.	0:144	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	12	43	theme	anticalcification	1712:1728	arg1	agent					1730:1734	a promising anticalcification agent	1700:1734	a promising anticalcification agent	1700:1734	Histological data confirmed the obtained results, suggesting that heparin treatment is a promising anticalcification agent.					
30413947	12	43	theme	anticalcification	1712:1728	arg1	treatment					1687:1695	heparin treatment	1679:1695	heparin treatment	1679:1695	Histological data confirmed the obtained results, suggesting that heparin treatment is a promising anticalcification agent.					
30413947	8	44	theme	raw	1152:1154	arg1	heparin					1156:1162	raw heparin	1152:1162	raw heparin	1152:1162	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	11	45	from	heparin	1590:1596	arg1	similar					1555:1561	similar	1555:1561	similar	1555:1561	Calcification inhibition potential was very similar in both the second and raw heparin (third group).					
30413947	10	46	dep	control	1488:1494	arg1	group					1503:1507	first group	1497:1507	first group	1497:1507	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	0	47	theme	hydrogel	22:29	arg1	embedding					31:39	heparin hydrogel embedding	14:39	heparin hydrogel embedding	14:39	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	9	48	theme	mechanical	1295:1304	arg1	behavior					1306:1313	its mechanical behavior	1291:1313	its mechanical behavior	1291:1313	The results showed that embedding of tissue with hydrogel had no stiffening effect on its mechanical behavior.					
30413947	2	49	theme	free	252:255	arg1	alternatives					284:295	free of anticoagulation therapy alternatives	252:295	free of anticoagulation therapy alternatives to mechanical substitutes	252:321	Bioprosthetic heart valves, introduced as free of anticoagulation therapy alternatives to mechanical substitutes.					
30413947	8	50	theme	calcification	1006:1018	arg1	experiments					1020:1030	the calcification experiments	1002:1030	the calcification experiments	1002:1030	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	10	51	theme	significant	1350:1360	arg1	efficacy					1362:1369	a significant efficacy	1348:1369	a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group)	1348:1450	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	0	52	theme	glutaraldehyde	44:57	arg1	tissues					84:90	glutaraldehyde fixed bovine pericardial tissues	44:90	glutaraldehyde fixed bovine pericardial tissues	44:90	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	8	53	with	Tissues	1139:1145	arg1	heparin					1156:1162	raw heparin	1152:1162	raw heparin	1152:1162	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	8	54	theme	calcification	1181:1193	arg1	solution					1195:1202	the calcification solution	1177:1202	the calcification solution	1177:1202	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	10	55	theme	hydrogel	1420:1427	arg1	deposition					1406:1415	calcium phosphate deposition	1388:1415	calcium phosphate deposition of hydrogel treated (second group)	1388:1450	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	7	56	theme	fixed	839:843	arg1	tissues					830:836	heparin hydrogel embedded bovine pericardial tissues	785:836	heparin hydrogel embedded bovine pericardial tissues	785:836	After that, heparin hydrogel embedded bovine pericardial tissues, fixed with glutaraldehyde, were produced and tested for their mechanical behavior and anticalcifcation potential in vitro using the constant composition model.					
30413947	3	57	theme	long	347:350	arg1	failure					357:363	long term failure	347:363	long term failure due to calcification	347:384	Still they suffer from long term failure due to calcification.					
30413947	10	58	from	efficacy	1362:1369	arg1	inhibition					1374:1383	inhibition	1374:1383	inhibition of calcium phosphate deposition of hydrogel treated (second group)	1374:1450	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	4	59	theme	treatment	397:405	arg1	methods					407:413	Different treatment methods	387:413	Different treatment methods	387:413	Different treatment methods introduced to inhibit calcification, have so far been limited in success.					
30413947	12	60	theme	obtained	1645:1652	arg1	results					1654:1660	the obtained results	1641:1660	the obtained results	1641:1660	Histological data confirmed the obtained results, suggesting that heparin treatment is a promising anticalcification agent.					
30413947	4	61	theme	Different	387:395	arg1	methods					407:413	Different treatment methods	387:413	Different treatment methods	387:413	Different treatment methods introduced to inhibit calcification, have so far been limited in success.					
30413947	8	62	dep	Tissues	1139:1145	arg1	c					1136:1136	c	1136:1136	c	1136:1136	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	10	63	theme	deposition	1406:1415	arg1	inhibition					1374:1383	inhibition	1374:1383	inhibition of calcium phosphate deposition of hydrogel treated (second group)	1374:1450	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	2	64	theme	heart	224:228	arg1	valves					230:235	Bioprosthetic heart valves	210:235	Bioprosthetic heart valves	210:235	Bioprosthetic heart valves, introduced as free of anticoagulation therapy alternatives to mechanical substitutes.					
30413947	12	65	theme	promising	1702:1710	arg1	agent					1730:1734	a promising anticalcification agent	1700:1734	a promising anticalcification agent	1700:1734	Histological data confirmed the obtained results, suggesting that heparin treatment is a promising anticalcification agent.					
30413947	12	65	theme	promising	1702:1710	arg1	treatment					1687:1695	heparin treatment	1679:1695	heparin treatment	1679:1695	Histological data confirmed the obtained results, suggesting that heparin treatment is a promising anticalcification agent.					
30413947	1	66	from	countries	199:207	arg1	common					174:179	common	174:179	common	174:179	Heart valve diseases remain common in industrialized countries.					
30413947	1	67	theme	Heart	146:150	arg1	diseases					158:165	Heart valve diseases	146:165	Heart valve diseases	146:165	Heart valve diseases remain common in industrialized countries.					
30413947	7	68	theme	composition	980:990	arg1	model					992:996	the constant composition model	967:996	the constant composition model	967:996	After that, heparin hydrogel embedded bovine pericardial tissues, fixed with glutaraldehyde, were produced and tested for their mechanical behavior and anticalcifcation potential in vitro using the constant composition model.					
30413947	8	69	dep	Controls	1076:1083	arg1	a					1073:1073	a	1073:1073	a	1073:1073	In the calcification experiments, tissues were divided into three groups: a) Controls without treatment, b) Hydrogel treated tissues and c) Tissues with raw heparin dissolved in the calcification solution.					
30413947	0	70	theme	bovine	65:70	arg1	tissues					84:90	glutaraldehyde fixed bovine pericardial tissues	44:90	glutaraldehyde fixed bovine pericardial tissues	44:90	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	2	71	theme	Bioprosthetic	210:222	arg1	valves					230:235	Bioprosthetic heart valves	210:235	Bioprosthetic heart valves	210:235	Bioprosthetic heart valves, introduced as free of anticoagulation therapy alternatives to mechanical substitutes.					
30413947	2	72	theme	therapy	276:282	arg1	free					252:255	free	252:255	free	252:255	Bioprosthetic heart valves, introduced as free of anticoagulation therapy alternatives to mechanical substitutes.					
30413947	1	73	theme	valve	152:156	arg1	diseases					158:165	Heart valve diseases	146:165	Heart valve diseases	146:165	Heart valve diseases remain common in industrialized countries.					
30413947	10	74	dep	tissues	1479:1485	arg1	comparison					1455:1464	comparison	1455:1464	comparison	1455:1464	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	7	75	theme	bovine	811:816	arg1	tissues					830:836	heparin hydrogel embedded bovine pericardial tissues	785:836	heparin hydrogel embedded bovine pericardial tissues	785:836	After that, heparin hydrogel embedded bovine pericardial tissues, fixed with glutaraldehyde, were produced and tested for their mechanical behavior and anticalcifcation potential in vitro using the constant composition model.					
30413947	0	76	theme	fixed	59:63	arg1	tissues					84:90	glutaraldehyde fixed bovine pericardial tissues	44:90	glutaraldehyde fixed bovine pericardial tissues	44:90	The effect of heparin hydrogel embedding on glutaraldehyde fixed bovine pericardial tissues: Mechanical behavior and anticalcification potential.					
30413947	2	77	theme	anticoagulation	260:274	arg1	therapy					276:282	anticoagulation therapy	260:282	anticoagulation therapy	260:282	Bioprosthetic heart valves, introduced as free of anticoagulation therapy alternatives to mechanical substitutes.					
30413947	10	78	theme	second	1438:1443	arg1	group					1445:1449	second group	1438:1449	second group	1438:1449	Calcification assessment showed a significant efficacy on inhibition of calcium phosphate deposition of hydrogel treated (second group) in comparison to untreated tissues (control, first group).					
30413947	2	79	dep	mechanical	300:309	arg1	substitutes					311:321	substitutes	311:321	substitutes	311:321	Bioprosthetic heart valves, introduced as free of anticoagulation therapy alternatives to mechanical substitutes.					
30413947	6	80	theme	heparin	691:697	arg1	hydrogel					699:706	heparin hydrogel	691:706	heparin hydrogel	691:706	In this study, heparin hydrogel was prepared and characterized both chemically and mechanically.					
30413947	7	81	theme	pericardial	818:828	arg1	tissues					830:836	heparin hydrogel embedded bovine pericardial tissues	785:836	heparin hydrogel embedded bovine pericardial tissues	785:836	After that, heparin hydrogel embedded bovine pericardial tissues, fixed with glutaraldehyde, were produced and tested for their mechanical behavior and anticalcifcation potential in vitro using the constant composition model.					
29728388	9	0	from	involvement	1823:1833	arg1	development					1869:1879	the development	1865:1879	the development of UC	1865:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	9	0	from	involvement	1823:1833	arg1	UC					1812:1813	UC	1812:1813	UC	1812:1813	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	6	1	theme	UC	1071:1072	arg1	conditions					1074:1083	UC conditions	1071:1083	UC conditions	1071:1083	Gut microbiota under normal conditions could maintain stable species richness and diversity upon FMT intervention, but the disturbed gut microbiota under UC conditions could not maintain such homeostasis.					
29728388	3	2	theme	gut	486:488	arg1	composition					510:520	gut microbial community composition	486:520	gut microbial community composition	486:520	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	8	3	theme	metabolic	1675:1683	arg1	association					1685:1695	gut bacterial and host metabolic association	1652:1695	association	1685:1695	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	9	4	from	development	1869:1879	arg1	understanding					1774:1786	our understanding	1770:1786	our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC	1770:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	8	5	theme	FMT-treated	1718:1728	arg1	UC					1743:1744	FMT-treated experimental UC	1718:1744	FMT-treated experimental UC	1718:1744	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	7	6	theme	variance	1295:1302	arg1	%					1284:1284	8.0 to 16.2%	1273:1284	8.0 to 16.2% of total variance	1273:1302	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	7	6	theme	variance	1295:1302	arg1	variance					1295:1302	total variance	1289:1302	total variance	1289:1302	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	7	7	theme	variance	1363:1370	arg1	variance					1363:1370	total variance	1357:1370	total variance	1357:1370	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	7	7	theme	variance	1363:1370	arg1	%					1352:1352	3.9 to 7.0%	1342:1352	3.9 to 7.0% of total variance	1342:1370	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	4	8	theme	normal	651:656	arg1	donors					658:663	normal donors	651:663	normal donors	651:663	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	3	9	theme	sulfate	557:563	arg1	model					587:591	the dextran sulfate sodium-induced UC rat model	545:591	the dextran sulfate sodium-induced UC rat model	545:591	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	8	10	theme	experimental	1730:1741	arg1	UC					1743:1744	FMT-treated experimental UC	1718:1744	FMT-treated experimental UC	1718:1744	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	5	11	theme	UC	849:850	arg1	symptoms					852:859	UC symptoms	849:859	UC symptoms	849:859	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	6	12	theme	normal	938:943	arg1	conditions					945:954	normal conditions	938:954	normal conditions	938:954	Gut microbiota under normal conditions could maintain stable species richness and diversity upon FMT intervention, but the disturbed gut microbiota under UC conditions could not maintain such homeostasis.					
29728388	4	13	theme	pilot	617:621	arg1	study					623:627	this pilot study	612:627	this pilot study	612:627	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	2	14	theme	detailed	333:340	arg1	basis					366:370	the detailed microbial and molecular basis	329:370	the detailed microbial and molecular basis	329:370	Data from individual case studies have suggested that FMT may be beneficial for UC, but the detailed microbial and molecular basis remains unknown.					
29728388	1	15	theme	ulcerative	216:225	arg1	UC					236:237	UC	236:237	UC	236:237	Fecal microbiota transplantation (FMT) is gaining attention for the treatment of ulcerative colitis (UC).					
29728388	1	15	theme	ulcerative	216:225	arg1	colitis					227:233	ulcerative colitis	216:233	ulcerative colitis (UC)	216:238	Fecal microbiota transplantation (FMT) is gaining attention for the treatment of ulcerative colitis (UC).					
29728388	5	16	theme	normal	817:822	arg1	rats					834:837	normal recipient rats	817:837	normal recipient rats	817:837	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	8	17	theme	diverse	1401:1407	arg1	shifts					1423:1428	diverse gut microbial shifts	1401:1428	diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC	1401:1744	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	3	18	theme	UC	580:581	arg1	model					587:591	the dextran sulfate sodium-induced UC rat model	545:591	the dextran sulfate sodium-induced UC rat model	545:591	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	1	19	theme	microbiota	141:150	arg1	transplantation					152:166	Fecal microbiota transplantation	135:166	Fecal microbiota transplantation (FMT)	135:172	Fecal microbiota transplantation (FMT) is gaining attention for the treatment of ulcerative colitis (UC).					
29728388	1	19	theme	microbiota	141:150	arg1	FMT					169:171	FMT	169:171	FMT	169:171	Fecal microbiota transplantation (FMT) is gaining attention for the treatment of ulcerative colitis (UC).					
29728388	8	20	theme	microbial	1413:1421	arg1	shifts					1423:1428	diverse gut microbial shifts	1401:1428	diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC	1401:1744	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	7	21	theme	effects	1333:1339	arg1	effects					1243:1249	the pathological effects	1226:1249	the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance)	1226:1371	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	8	22	dep	bacterial	1656:1664	arg1	the					1648:1650	the	1648:1650	the	1648:1650	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	2	23	theme	case	262:265	arg1	studies					267:273	individual case studies	251:273	individual case studies	251:273	Data from individual case studies have suggested that FMT may be beneficial for UC, but the detailed microbial and molecular basis remains unknown.					
29728388	5	24	theme	host	892:895	arg1	adaption					907:914	host metabolic adaption	892:914	host metabolic adaption	892:914	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	8	25	theme	host	1670:1673	arg1	association					1685:1695	gut bacterial and host metabolic association	1652:1695	association	1685:1695	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	7	26	theme	FMT	1316:1318	arg1	effects					1333:1339	the FMT intervention effects	1312:1339	the FMT intervention effects (3.9 to 7.0% of total variance)	1312:1371	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	7	27	theme	pathological	1230:1241	arg1	effects					1243:1249	the pathological effects	1226:1249	the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance)	1226:1371	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	0	28	from	Transplantation	17:31	arg1	Colitis					60:66	Experimental Ulcerative Colitis	36:66	Experimental Ulcerative Colitis	36:66	Fecal Microbiota Transplantation in Experimental Ulcerative Colitis Reveals Associated Gut Microbial and Host Metabolic Reprogramming.					
29728388	5	29	theme	microbial	871:879	arg1	shift					881:885	UC-prone microbial shift	862:885	UC-prone microbial shift	862:885	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	6	30	theme	stable	971:976	arg1	richness					986:993	stable species richness	971:993	stable species richness	971:993	Gut microbiota under normal conditions could maintain stable species richness and diversity upon FMT intervention, but the disturbed gut microbiota under UC conditions could not maintain such homeostasis.					
29728388	0	31	theme	Host	105:108	arg1	Reprogramming					120:132	Associated Gut Microbial and Host Metabolic Reprogramming	76:132	Reprogramming	120:132	Fecal Microbiota Transplantation in Experimental Ulcerative Colitis Reveals Associated Gut Microbial and Host Metabolic Reprogramming.					
29728388	4	32	theme	microbial	751:759	arg1	pattern					775:781	donor's gut microbial and metabolic pattern	739:781	donor's gut microbial and metabolic pattern	739:781	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	3	33	theme	gene	414:417	arg1	sequencing					419:428	16S rRNA gene sequencing	405:428	16S rRNA gene sequencing	405:428	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	6	34	theme	gut	1050:1052	arg1	microbiota					1054:1063	the disturbed gut microbiota	1036:1063	the disturbed gut microbiota under UC conditions	1036:1083	Gut microbiota under normal conditions could maintain stable species richness and diversity upon FMT intervention, but the disturbed gut microbiota under UC conditions could not maintain such homeostasis.					
29728388	9	35	theme	effect	1795:1800	arg1	understanding					1774:1786	our understanding	1770:1786	our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC	1770:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	8	36	theme	host	1475:1478	arg1	reprogramming.IMPORTANCE					1490:1513	host metabolic reprogramming.IMPORTANCE	1475:1513	host metabolic reprogramming.IMPORTANCE	1475:1513	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	4	37	theme	close	718:722	arg1	resemblance					724:734	close resemblance	718:734	close resemblance of donor's gut microbial and metabolic pattern	718:781	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	3	38	theme	16S	405:407	arg1	sequencing					419:428	16S rRNA gene sequencing	405:428	16S rRNA gene sequencing	405:428	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	0	39	theme	Fecal	0:4	arg1	Transplantation					17:31	Fecal Microbiota Transplantation	0:31	Fecal Microbiota Transplantation in Experimental Ulcerative Colitis	0:66	Fecal Microbiota Transplantation in Experimental Ulcerative Colitis Reveals Associated Gut Microbial and Host Metabolic Reprogramming.					
29728388	9	40	theme	FMT	1805:1807	arg1	effect					1795:1800	the effect	1791:1800	the effect of FMT on UC	1791:1813	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	9	40	theme	FMT	1805:1807	arg1	involvement					1823:1833	the involvement	1819:1833	the involvement of gut microbial dysbiosis in the development of UC	1819:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	7	41	theme	bacterial	1163:1171	arg1	composition					1173:1183	altered bacterial composition	1155:1183	altered bacterial composition	1155:1183	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	8	42	dep	shifts	1423:1428	arg1	combined					1526:1533	combined	1526:1533	combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC	1526:1744	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	2	43	from	studies	267:273	arg1	Data					241:244	Data	241:244	Data from individual case studies	241:273	Data from individual case studies have suggested that FMT may be beneficial for UC, but the detailed microbial and molecular basis remains unknown.					
29728388	7	44	theme	total	1289:1293	arg1	variance					1295:1302	total variance	1289:1302	total variance	1289:1302	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	0	45	theme	Experimental	36:47	arg1	Colitis					60:66	Experimental Ulcerative Colitis	36:66	Experimental Ulcerative Colitis	36:66	Fecal Microbiota Transplantation in Experimental Ulcerative Colitis Reveals Associated Gut Microbial and Host Metabolic Reprogramming.					
29728388	6	46	theme	FMT	1014:1016	arg1	intervention					1018:1029	FMT intervention	1014:1029	FMT intervention	1014:1029	Gut microbiota under normal conditions could maintain stable species richness and diversity upon FMT intervention, but the disturbed gut microbiota under UC conditions could not maintain such homeostasis.					
29728388	8	47	theme	clinical	1535:1542	arg1	symptoms					1544:1551	clinical symptoms	1535:1551	clinical symptoms	1535:1551	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	7	48	theme	Significant	1122:1132	arg1	correlations					1134:1145	Significant correlations	1122:1145	Significant correlations between altered bacterial composition and host metabolism	1122:1203	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	6	49	theme	such	1104:1107	arg1	homeostasis					1109:1119	such homeostasis	1104:1119	such homeostasis	1104:1119	Gut microbiota under normal conditions could maintain stable species richness and diversity upon FMT intervention, but the disturbed gut microbiota under UC conditions could not maintain such homeostasis.					
29728388	4	50	theme	metabolic	765:773	arg1	pattern					775:781	donor's gut microbial and metabolic pattern	739:781	donor's gut microbial and metabolic pattern	739:781	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	9	51	theme	microbial	1842:1850	arg1	dysbiosis					1852:1860	gut microbial dysbiosis	1838:1860	gut microbial dysbiosis	1838:1860	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	8	52	theme	16S	1566:1568	arg1	profiling					1590:1598	16S rRNA gene microbial profiling	1566:1598	16S rRNA gene microbial profiling	1566:1598	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	3	53	theme	microbial	490:498	arg1	composition					510:520	gut microbial community composition	486:520	gut microbial community composition	486:520	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	8	54	theme	gene	1575:1578	arg1	profiling					1590:1598	16S rRNA gene microbial profiling	1566:1598	16S rRNA gene microbial profiling	1566:1598	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	2	55	theme	microbial	342:350	arg1	basis					366:370	the detailed microbial and molecular basis	329:370	the detailed microbial and molecular basis	329:370	Data from individual case studies have suggested that FMT may be beneficial for UC, but the detailed microbial and molecular basis remains unknown.					
29728388	8	56	theme	related	1436:1442	arg1	FMT					1444:1446	UC related FMT	1433:1446	UC related FMT	1433:1446	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	9	57	from	UC	1812:1813	arg1	understanding					1774:1786	our understanding	1770:1786	our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC	1770:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	3	58	theme	host	526:529	arg1	metabolism					531:540	host metabolism	526:540	host metabolism	526:540	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	4	59	from	donors	658:663	arg1	FMT					642:644	FMT	642:644	FMT from normal donors to UC recipients	642:680	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	9	60	theme	UC	1884:1885	arg1	development					1869:1879	the development	1865:1879	the development of UC	1865:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	5	61	theme	recipient	824:832	arg1	rats					834:837	normal recipient rats	817:837	normal recipient rats	817:837	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	9	62	from	effect	1795:1800	arg1	development					1869:1879	the development	1865:1879	the development of UC	1865:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	9	62	from	effect	1795:1800	arg1	UC					1812:1813	UC	1812:1813	UC	1812:1813	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	3	63	from	influence	466:474	arg1	composition					510:520	gut microbial community composition	486:520	gut microbial community composition	486:520	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	3	63	from	influence	466:474	arg1	metabolism					531:540	host metabolism	526:540	host metabolism	526:540	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	3	64	theme	dextran	549:555	arg1	model					587:591	the dextran sulfate sodium-induced UC rat model	545:591	the dextran sulfate sodium-induced UC rat model	545:591	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	7	65	dep	7.0	1349:1351	arg1	to					1346:1347	to	1346:1347	to	1346:1347	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	8	66	with	FMT	1444:1446	arg1	reprogramming.IMPORTANCE					1490:1513	host metabolic reprogramming.IMPORTANCE	1475:1513	host metabolic reprogramming.IMPORTANCE	1475:1513	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	4	67	from	study	623:627	arg1	findings					598:605	The findings	594:605	The findings from this pilot study	594:627	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	5	68	theme	UC	804:805	arg1	donors					807:812	UC donors	804:812	UC donors	804:812	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	7	69	theme	total	1357:1361	arg1	variance					1363:1370	total variance	1357:1370	total variance	1357:1370	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	3	70	theme	rat	583:585	arg1	model					587:591	the dextran sulfate sodium-induced UC rat model	545:591	the dextran sulfate sodium-induced UC rat model	545:591	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	7	71	dep	effects	1333:1339	arg1	variance					1363:1370	total variance	1357:1370	total variance	1357:1370	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	7	71	dep	effects	1333:1339	arg1	%					1352:1352	3.9 to 7.0%	1342:1352	3.9 to 7.0% of total variance	1342:1370	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	6	72	theme	Gut	917:919	arg1	microbiota					921:930	Gut microbiota	917:930	Gut microbiota under normal conditions	917:954	Gut microbiota under normal conditions could maintain stable species richness and diversity upon FMT intervention, but the disturbed gut microbiota under UC conditions could not maintain such homeostasis.					
29728388	8	73	theme	gut	1409:1411	arg1	shifts					1423:1428	diverse gut microbial shifts	1401:1428	diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC	1401:1744	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	3	74	theme	sodium-induced	565:578	arg1	model					587:591	the dextran sulfate sodium-induced UC rat model	545:591	the dextran sulfate sodium-induced UC rat model	545:591	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	1	75	theme	Fecal	135:139	arg1	transplantation					152:166	Fecal microbiota transplantation	135:166	Fecal microbiota transplantation (FMT)	135:172	Fecal microbiota transplantation (FMT) is gaining attention for the treatment of ulcerative colitis (UC).					
29728388	1	75	theme	Fecal	135:139	arg1	FMT					169:171	FMT	169:171	FMT	169:171	Fecal microbiota transplantation (FMT) is gaining attention for the treatment of ulcerative colitis (UC).					
29728388	5	76	from	donors	807:812	arg1	FMT					795:797	FMT	795:797	FMT from UC donors to normal recipient rats	795:837	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	5	76	from	donors	807:812	arg1	Meanwhile					784:792	Meanwhile	784:792	Meanwhile	784:792	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	8	77	theme	UC	1433:1434	arg1	FMT					1444:1446	UC related FMT	1433:1446	UC related FMT	1433:1446	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	4	78	theme	UC	698:699	arg1	symptoms					701:708	UC symptoms	698:708	UC symptoms	698:708	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	2	79	theme	individual	251:260	arg1	studies					267:273	individual case studies	251:273	individual case studies	251:273	Data from individual case studies have suggested that FMT may be beneficial for UC, but the detailed microbial and molecular basis remains unknown.					
29728388	4	80	dep	microbial	751:759	arg1	's					744:745	's	744:745	's	744:745	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	7	81	theme	intervention	1320:1331	arg1	effects					1333:1339	the FMT intervention effects	1312:1339	the FMT intervention effects (3.9 to 7.0% of total variance)	1312:1371	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	5	82	theme	UC-prone	862:869	arg1	shift					881:885	UC-prone microbial shift	862:885	UC-prone microbial shift	862:885	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	6	83	theme	species	978:984	arg1	richness					986:993	stable species richness	971:993	stable species richness	971:993	Gut microbiota under normal conditions could maintain stable species richness and diversity upon FMT intervention, but the disturbed gut microbiota under UC conditions could not maintain such homeostasis.					
29728388	4	84	theme	UC	668:669	arg1	recipients					671:680	UC recipients	668:680	UC recipients	668:680	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	0	85	theme	Metabolic	110:118	arg1	Reprogramming					120:132	Associated Gut Microbial and Host Metabolic Reprogramming	76:132	Reprogramming	120:132	Fecal Microbiota Transplantation in Experimental Ulcerative Colitis Reveals Associated Gut Microbial and Host Metabolic Reprogramming.					
29728388	7	86	theme	host	1189:1192	arg1	metabolism					1194:1203	host metabolism	1189:1203	host metabolism	1189:1203	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	8	87	theme	metabolic	1480:1488	arg1	reprogramming.IMPORTANCE					1490:1513	host metabolic reprogramming.IMPORTANCE	1475:1513	host metabolic reprogramming.IMPORTANCE	1475:1513	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	8	88	from	shifts	1423:1428	arg1	FMT					1444:1446	UC related FMT	1433:1446	UC related FMT	1433:1446	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	8	88	from	shifts	1423:1428	arg1	association					1458:1468	their association	1452:1468	their association with host metabolic reprogramming.IMPORTANCE	1452:1513	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	0	89	theme	Microbiota	6:15	arg1	Transplantation					17:31	Fecal Microbiota Transplantation	0:31	Fecal Microbiota Transplantation in Experimental Ulcerative Colitis	0:66	Fecal Microbiota Transplantation in Experimental Ulcerative Colitis Reveals Associated Gut Microbial and Host Metabolic Reprogramming.					
29728388	5	90	theme	metabolic	897:905	arg1	adaption					907:914	host metabolic adaption	892:914	host metabolic adaption	892:914	Meanwhile, FMT from UC donors to normal recipient rats triggered UC symptoms, UC-prone microbial shift, and host metabolic adaption.					
29728388	6	91	theme	disturbed	1040:1048	arg1	microbiota					1054:1063	the disturbed gut microbiota	1036:1063	the disturbed gut microbiota under UC conditions	1036:1083	Gut microbiota under normal conditions could maintain stable species richness and diversity upon FMT intervention, but the disturbed gut microbiota under UC conditions could not maintain such homeostasis.					
29728388	3	92	theme	rRNA	409:412	arg1	sequencing					419:428	16S rRNA gene sequencing	405:428	16S rRNA gene sequencing	405:428	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	7	93	theme	altered	1155:1161	arg1	composition					1173:1183	altered bacterial composition	1155:1183	altered bacterial composition	1155:1183	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	8	94	from	bacterial	1656:1664	arg1	UC					1743:1744	FMT-treated experimental UC	1718:1744	FMT-treated experimental UC	1718:1744	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	2	95	theme	molecular	356:364	arg1	basis					366:370	the detailed microbial and molecular basis	329:370	the detailed microbial and molecular basis	329:370	Data from individual case studies have suggested that FMT may be beneficial for UC, but the detailed microbial and molecular basis remains unknown.					
29728388	0	96	theme	Ulcerative	49:58	arg1	Colitis					60:66	Experimental Ulcerative Colitis	36:66	Experimental Ulcerative Colitis	36:66	Fecal Microbiota Transplantation in Experimental Ulcerative Colitis Reveals Associated Gut Microbial and Host Metabolic Reprogramming.					
29728388	1	97	theme	colitis	227:233	arg1	treatment					203:211	the treatment	199:211	the treatment of ulcerative colitis (UC)	199:238	Fecal microbiota transplantation (FMT) is gaining attention for the treatment of ulcerative colitis (UC).					
29728388	8	98	with	association	1458:1468	arg1	reprogramming.IMPORTANCE					1490:1513	host metabolic reprogramming.IMPORTANCE	1475:1513	host metabolic reprogramming.IMPORTANCE	1475:1513	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	9	99	from	understanding	1774:1786	arg1	development					1869:1879	the development	1865:1879	the development of UC	1865:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	9	99	from	understanding	1774:1786	arg1	UC					1812:1813	UC	1812:1813	UC	1812:1813	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	9	100	theme	involvement	1823:1833	arg1	understanding					1774:1786	our understanding	1770:1786	our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC	1770:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	3	101	theme	FMT	479:481	arg1	influence					466:474	the influence	462:474	the influence of FMT on gut microbial community composition and host metabolism	462:540	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29728388	7	102	dep	16.2	1280:1283	arg1	to					1277:1278	to	1277:1278	to	1277:1278	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	9	103	theme	gut	1838:1840	arg1	dysbiosis					1852:1860	gut microbial dysbiosis	1838:1860	gut microbial dysbiosis	1838:1860	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	8	104	theme	rRNA	1570:1573	arg1	profiling					1590:1598	16S rRNA gene microbial profiling	1566:1598	16S rRNA gene microbial profiling	1566:1598	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	8	105	from	UC	1743:1744	arg1	bacterial					1656:1664	bacterial	1656:1664	bacterial	1656:1664	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	7	106	theme	UC	1254:1255	arg1	effects					1243:1249	the pathological effects	1226:1249	the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance)	1226:1371	Significant correlations between altered bacterial composition and host metabolism could be assigned to the pathological effects of UC (accounting for 8.0 to 16.2% of total variance) and/or the FMT intervention effects (3.9 to 7.0% of total variance).					
29728388	9	107	theme	dysbiosis	1852:1860	arg1	effect					1795:1800	the effect	1791:1800	the effect of FMT on UC	1791:1813	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	9	107	theme	dysbiosis	1852:1860	arg1	involvement					1823:1833	the involvement	1819:1833	the involvement of gut microbial dysbiosis in the development of UC	1819:1885	These data can advance our understanding of the effect of FMT on UC and the involvement of gut microbial dysbiosis in the development of UC.					
29728388	8	108	theme	microbial	1580:1588	arg1	profiling					1590:1598	16S rRNA gene microbial profiling	1566:1598	16S rRNA gene microbial profiling	1566:1598	Overall, our study reveals diverse gut microbial shifts in UC related FMT and their association with host metabolic reprogramming.IMPORTANCE This study combined clinical symptoms measurement, 16S rRNA gene microbial profiling and metabolomics to comprehensively investigate the gut bacterial and host metabolic association and reprogramming in FMT-treated experimental UC.					
29728388	4	109	theme	pattern	775:781	arg1	resemblance					724:734	close resemblance	718:734	close resemblance of donor's gut microbial and metabolic pattern	718:781	The findings from this pilot study suggest that FMT from normal donors to UC recipients could alleviate UC symptoms without close resemblance of donor's gut microbial and metabolic pattern.					
29728388	3	110	theme	community	500:508	arg1	composition					510:520	gut microbial community composition	486:520	gut microbial community composition	486:520	Here, we employ 16S rRNA gene sequencing and metabolomics to investigate the influence of FMT on gut microbial community composition and host metabolism in the dextran sulfate sodium-induced UC rat model.					
29315662	6	0	theme	maturation	1186:1195	arg1	stages					1197:1202	maturation stages	1186:1202	maturation stages	1186:1202	Thus, the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China was quantified with the proposed method.					
29315662	3	1	theme	double	846:851	arg1	equation					864:871	double reciprocal equation	846:871	double reciprocal equation	846:871	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	4	2	theme	proposed	922:929	arg1	method					931:936	The proposed method	918:936	The proposed method	918:936	The proposed method allowed the LODs of PTAs were averaged at 1.50 μg/mL with UV detection (at 200 nm).					
29315662	5	3	theme	time	1052:1055	arg1	RSD					1035:1037	The interday RSD	1022:1037	The interday RSD of migration time and peak area	1022:1069	The interday RSD of migration time and peak area were around 2.0 and 4.7% (n = 5), respectively.					
29315662	5	3	theme	time	1052:1055	arg1	%					1094:1094	around 2.0 and 4.7%	1076:1094	around 2.0 and 4.7% (n = 5)	1076:1102	The interday RSD of migration time and peak area were around 2.0 and 4.7% (n = 5), respectively.					
29315662	1	4	theme	oleanolic	353:361	arg1	OA					369:370	OA	369:370	OA	369:370	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	4	theme	oleanolic	353:361	arg1	acid					363:366	oleanolic acid	353:366	oleanolic acid (OA)	353:371	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	6	5	theme	real	1154:1157	arg1	samples					1159:1165	19 FF real samples	1148:1165	19 FF real samples distinguished from maturation stages and geographical areas in China	1148:1234	Thus, the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China was quantified with the proposed method.					
29315662	7	6	theme	PTA	1309:1311	arg1	amount					1294:1299	the amount	1290:1299	the amount of each PTA in FF	1290:1317	Depending on the amount of each PTA in FF, it was demonstrated these microconstituents might benefit to identify their harvested time even qualities.					
29315662	7	6	theme	PTA	1309:1311	arg1	PTA					1309:1311	each PTA	1304:1311	each PTA	1304:1311	Depending on the amount of each PTA in FF, it was demonstrated these microconstituents might benefit to identify their harvested time even qualities.					
29315662	2	7	theme	PTAs	472:475	arg1	separation					458:467	The baseline separation	445:467	The baseline separation of PTAs by CZE	445:482	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	0	8	from	Study	0:4	arg1	constants					119:127	their binding constants	105:127	their binding constants with β-cyclodextrin by capillary electrophoresis	105:176	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	0	8	from	Study	0:4	arg1	medicine					70:77	traditional Chinese medicine	50:77	traditional Chinese medicine of Forsythiae Fructus	50:99	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	4	9	dep	allowed	938:944	arg1	200 nm					1013:1018	200 nm	1013:1018	200 nm	1013:1018	The proposed method allowed the LODs of PTAs were averaged at 1.50 μg/mL with UV detection (at 200 nm).					
29315662	3	10	theme	equilibrium	775:785	arg1	K					798:798	K	798:798	K	798:798	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	3	10	theme	equilibrium	775:785	arg1	constants					787:795	the equilibrium constants	771:795	the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism	771:915	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	5	11	theme	peak	1061:1064	arg1	area					1066:1069	peak area	1061:1069	peak area	1061:1069	The interday RSD of migration time and peak area were around 2.0 and 4.7% (n = 5), respectively.					
29315662	0	12	theme	Fructus	93:99	arg1	constants					119:127	their binding constants	105:127	their binding constants with β-cyclodextrin by capillary electrophoresis	105:176	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	0	12	theme	Fructus	93:99	arg1	medicine					70:77	traditional Chinese medicine	50:77	traditional Chinese medicine of Forsythiae Fructus	50:99	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	1	13	dep	acids	331:335	arg1	PTAs					338:341	PTAs	338:341	PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)	338:414	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	13	dep	acids	331:335	arg1	acid					363:366	oleanolic acid	353:366	oleanolic acid (OA)	353:371	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	13	dep	acids	331:335	arg1	acid					382:385	ursolic acid	374:385	ursolic acid (UA)	374:390	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	13	dep	acids	331:335	arg1	acid					406:409	betulinic acid	396:409	betulinic acid (BA)	396:414	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	0	14	with	medicine	70:77	arg1	β-cyclodextrin					134:147	β-cyclodextrin	134:147	β-cyclodextrin by capillary electrophoresis	134:176	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	5	15	theme	area	1066:1069	arg1	RSD					1035:1037	The interday RSD	1022:1037	The interday RSD of migration time and peak area	1022:1069	The interday RSD of migration time and peak area were around 2.0 and 4.7% (n = 5), respectively.					
29315662	5	15	theme	area	1066:1069	arg1	%					1094:1094	around 2.0 and 4.7%	1076:1094	around 2.0 and 4.7% (n = 5)	1076:1102	The interday RSD of migration time and peak area were around 2.0 and 4.7% (n = 5), respectively.					
29315662	0	16	theme	binding	111:117	arg1	constants					119:127	their binding constants	105:127	their binding constants with β-cyclodextrin by capillary electrophoresis	105:176	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	7	17	theme	time	1406:1409	arg1	qualities					1416:1424	their harvested time even qualities	1390:1424	their harvested time even qualities	1390:1424	Depending on the amount of each PTA in FF, it was demonstrated these microconstituents might benefit to identify their harvested time even qualities.					
29315662	2	18	theme	0.5 mmol/L	576:585	arg1	β-CD					603:606	β-CD	603:606	β-CD	603:606	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	2	18	theme	0.5 mmol/L	576:585	arg1	β-cyclodextrin					587:600	0.5 mmol/L β-cyclodextrin	576:600	0.5 mmol/L β-cyclodextrin (β-CD)	576:607	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	4	19	theme	UV	996:997	arg1	detection					999:1007	UV detection	996:1007	UV detection	996:1007	The proposed method allowed the LODs of PTAs were averaged at 1.50 μg/mL with UV detection (at 200 nm).					
29315662	2	20	theme	baseline	449:456	arg1	separation					458:467	The baseline separation	445:467	The baseline separation of PTAs by CZE	445:482	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	7	21	theme	harvested	1396:1404	arg1	qualities					1416:1424	their harvested time even qualities	1390:1424	their harvested time even qualities	1390:1424	Depending on the amount of each PTA in FF, it was demonstrated these microconstituents might benefit to identify their harvested time even qualities.					
29315662	3	22	theme	BGE	680:682	arg1	compositions					664:675	the compositions	660:675	the compositions of BGE	660:682	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	3	23	dep	investigated	696:707	arg1	detail					689:694	detail	689:694	detail	689:694	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	2	24	theme	50.0 mmol/L	554:564	arg1	borax					566:570	50.0 mmol/L borax	554:570	50.0 mmol/L borax	554:570	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	4	25	with	1.50 μg/mL	980:989	arg1	detection					999:1007	UV detection	996:1007	UV detection	996:1007	The proposed method allowed the LODs of PTAs were averaged at 1.50 μg/mL with UV detection (at 200 nm).					
29315662	5	26	theme	interday	1026:1033	arg1	RSD					1035:1037	The interday RSD	1022:1037	The interday RSD of migration time and peak area	1022:1069	The interday RSD of migration time and peak area were around 2.0 and 4.7% (n = 5), respectively.					
29315662	5	26	theme	interday	1026:1033	arg1	%					1094:1094	around 2.0 and 4.7%	1076:1094	around 2.0 and 4.7% (n = 5)	1076:1102	The interday RSD of migration time and peak area were around 2.0 and 4.7% (n = 5), respectively.					
29315662	1	27	from	microconstituents	278:294	arg1	FF					440:441	FF	440:441	FF	440:441	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	27	from	microconstituents	278:294	arg1	Fructus					431:437	Fructus	431:437	Fructus	431:437	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	3	28	theme	separation	896:905	arg1	mechanism					907:915	the separation mechanism	892:915	the separation mechanism	892:915	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	0	29	theme	pentacyclic	18:28	arg1	acids					41:45	isomeric pentacyclic triterpene acids	9:45	isomeric pentacyclic triterpene acids	9:45	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	1	30	theme	capillary	196:204	arg1	CZE					228:230	CZE	228:230	CZE	228:230	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	30	theme	capillary	196:204	arg1	electrophoresis					211:225	capillary zone electrophoresis	196:225	a capillary zone electrophoresis (CZE) method	194:238	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	0	31	theme	isomeric	9:16	arg1	acids					41:45	isomeric pentacyclic triterpene acids	9:45	isomeric pentacyclic triterpene acids	9:45	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	1	32	theme	ursolic	374:380	arg1	UA					388:389	UA	388:389	UA	388:389	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	32	theme	ursolic	374:380	arg1	acid					382:385	ursolic acid	374:385	ursolic acid (UA)	374:390	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	33	theme	zone	206:209	arg1	CZE					228:230	CZE	228:230	CZE	228:230	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	33	theme	zone	206:209	arg1	electrophoresis					211:225	capillary zone electrophoresis	196:225	a capillary zone electrophoresis (CZE) method	194:238	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	0	34	theme	acids	41:45	arg1	Study					0:4	Study	0:4	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.	0:177	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	1	35	theme	isomeric	299:306	arg1	acids					331:335	isomeric pentacyclic triterpene acids	299:335	isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA))	299:415	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	6	36	theme	PTAs	1140:1143	arg1	content					1129:1135	the content	1125:1135	the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China	1125:1234	Thus, the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China was quantified with the proposed method.					
29315662	3	37	theme	binding	753:759	arg1	ratio					761:765	the binding ratio	749:765	the binding ratio	749:765	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	1	38	theme	electrophoresis	211:225	arg1	method					233:238	a capillary zone electrophoresis (CZE) method	194:238	a capillary zone electrophoresis (CZE) method	194:238	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	0	39	theme	triterpene	30:39	arg1	acids					41:45	isomeric pentacyclic triterpene acids	9:45	isomeric pentacyclic triterpene acids	9:45	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	1	40	theme	pentacyclic	308:318	arg1	acids					331:335	isomeric pentacyclic triterpene acids	299:335	isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA))	299:415	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	5	41	theme	migration	1042:1050	arg1	time					1052:1055	migration time	1042:1055	migration time	1042:1055	The interday RSD of migration time and peak area were around 2.0 and 4.7% (n = 5), respectively.					
29315662	6	42	theme	FF	1151:1152	arg1	samples					1159:1165	19 FF real samples	1148:1165	19 FF real samples distinguished from maturation stages and geographical areas in China	1148:1234	Thus, the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China was quantified with the proposed method.					
29315662	0	43	theme	traditional	50:60	arg1	medicine					70:77	traditional Chinese medicine	50:77	traditional Chinese medicine of Forsythiae Fructus	50:99	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	1	44	theme	triterpene	320:329	arg1	acids					331:335	isomeric pentacyclic triterpene acids	299:335	isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA))	299:415	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	2	45	theme	background	514:523	arg1	BGE					538:540	BGE	538:540	BGE	538:540	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	2	45	theme	background	514:523	arg1	electrolyte					525:535	a background electrolyte	512:535	a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5	512:617	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	6	46	theme	geographical	1208:1219	arg1	areas					1221:1225	geographical areas	1208:1225	geographical areas	1208:1225	Thus, the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China was quantified with the proposed method.					
29315662	1	47	theme	acids	331:335	arg1	microconstituents					278:294	three microconstituents	272:294	three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF)	272:442	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	0	48	theme	capillary	152:160	arg1	electrophoresis					162:176	capillary electrophoresis	152:176	capillary electrophoresis	152:176	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	6	49	from	areas	1221:1225	arg1	China					1230:1234	China	1230:1234	China	1230:1234	Thus, the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China was quantified with the proposed method.					
29315662	7	50	from	amount	1294:1299	arg1	FF					1316:1317	FF	1316:1317	FF	1316:1317	Depending on the amount of each PTA in FF, it was demonstrated these microconstituents might benefit to identify their harvested time even qualities.					
29315662	7	51	theme	even	1411:1414	arg1	qualities					1416:1424	their harvested time even qualities	1390:1424	their harvested time even qualities	1390:1424	Depending on the amount of each PTA in FF, it was demonstrated these microconstituents might benefit to identify their harvested time even qualities.					
29315662	3	52	dep	OA	805:806	arg1	estimated					833:841	estimated	833:841	was estimated by double reciprocal equation to well understand the separation mechanism	829:915	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	6	53	from	stages	1197:1202	arg1	China					1230:1234	China	1230:1234	China	1230:1234	Thus, the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China was quantified with the proposed method.					
29315662	1	54	theme	betulinic	396:404	arg1	BA					412:413	BA	412:413	BA	412:413	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	1	54	theme	betulinic	396:404	arg1	acid					406:409	betulinic acid	396:409	betulinic acid (BA)	396:414	In this study, a capillary zone electrophoresis (CZE) method was first developed to identify three microconstituents of isomeric pentacyclic triterpene acids (PTAs including oleanolic acid (OA), ursolic acid (UA) and betulinic acid (BA)) in Forsythiae Fructus (FF).					
29315662	3	55	theme	reciprocal	853:862	arg1	equation					864:871	double reciprocal equation	846:871	double reciprocal equation	846:871	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	3	56	theme	good	723:726	arg1	separation					728:737	rapid and good separation	713:737	rapid and good separation	713:737	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	6	57	theme	proposed	1260:1267	arg1	method					1269:1274	the proposed method	1256:1274	the proposed method	1256:1274	Thus, the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China was quantified with the proposed method.					
29315662	6	58	from	content	1129:1135	arg1	samples					1159:1165	19 FF real samples	1148:1165	19 FF real samples distinguished from maturation stages and geographical areas in China	1148:1234	Thus, the content of PTAs in 19 FF real samples distinguished from maturation stages and geographical areas in China was quantified with the proposed method.					
29315662	0	59	with	constants	119:127	arg1	β-cyclodextrin					134:147	β-cyclodextrin	134:147	β-cyclodextrin by capillary electrophoresis	134:176	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	0	60	theme	Chinese	62:68	arg1	medicine					70:77	traditional Chinese medicine	50:77	traditional Chinese medicine of Forsythiae Fructus	50:99	Study of isomeric pentacyclic triterpene acids in traditional Chinese medicine of Forsythiae Fructus and their binding constants with β-cyclodextrin by capillary electrophoresis.					
29315662	3	61	dep	only	655:658	arg1	not					651:653	not	651:653	not	651:653	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
29315662	2	62	contain	containing	543:552	arg1	BGE					538:540	BGE	538:540	BGE	538:540	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	2	62	contain	containing	543:552	arg1	electrolyte					525:535	a background electrolyte	512:535	a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5	512:617	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	2	62	contain	containing	543:552	arg2	borax					566:570	50.0 mmol/L borax	554:570	50.0 mmol/L borax	554:570	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	2	62	contain	containing	543:552	arg2	β-CD					603:606	β-CD	603:606	β-CD	603:606	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	2	62	contain	containing	543:552	arg2	β-cyclodextrin					587:600	0.5 mmol/L β-cyclodextrin	576:600	0.5 mmol/L β-cyclodextrin (β-CD)	576:607	The baseline separation of PTAs by CZE were eventually achieved in a background electrolyte (BGE) containing 50.0 mmol/L borax and 0.5 mmol/L β-cyclodextrin (β-CD) at pH 9.5 within 13.0 min.					
29315662	3	63	theme	rapid	713:717	arg1	separation					728:737	rapid and good separation	713:737	rapid and good separation	713:737	Herein, it was not only the compositions of BGE were detail investigated for rapid and good separation, but also the binding ratio and the equilibrium constants (K) for OA, UA and BA with β-CD was estimated by double reciprocal equation to well understand the separation mechanism.					
30210478	4	0	with	combination	941:951	arg1	spectrometry					963:974	mass spectrometry	958:974	mass spectrometry of the protein-released O-glycans	958:1008	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	1	1	theme	unique	270:275	arg1	decasaccharide					286:299	a unique, complex decasaccharide	268:299	a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins	268:405	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	10	2	theme	pentasaccharide	2306:2320	arg1	core					2322:2325	and the pentasaccharide core	2298:2325	core	2322:2325	This revealed that the O-glycan is pivotal to modulating DC effector functions, with the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response.					
30210478	7	3	theme	pathogenic	1808:1817	arg1	strains					1819:1825	the pathogenic strains	1804:1825	the pathogenic strains	1804:1825	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	8	4	theme	cell	1874:1877	arg1	glycosylation					1887:1899	differential cell surface glycosylation	1861:1899	differential cell surface glycosylation	1861:1899	Finally, we investigated whether differential cell surface glycosylation impacts T. forsythia's overall immunogenicity.					
30210478	6	5	theme	gene	1544:1547	arg1	clusters					1549:1556	gene clusters	1544:1556	gene clusters of similar size and organization as found in the ATCC 43037 type strain	1544:1628	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	3	6	theme	type	724:727	arg1	strain					729:734	the ATCC 43037 type strain	709:734	the ATCC 43037 type strain	709:734	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	11	7	theme	valuable	2473:2480	arg1	it					2465:2466	it	2465:2466	it	2465:2466	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	11	7	theme	valuable	2473:2480	arg1	target					2482:2487	a valuable target	2471:2487	a valuable target for the design of novel antimicrobials against periodontitis	2471:2548	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	10	8	theme	Th17	2340:2343	arg1	response					2345:2352	a Th17 response	2338:2352	a Th17 response	2338:2352	This revealed that the O-glycan is pivotal to modulating DC effector functions, with the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response.					
30210478	3	9	theme	gene	755:758	arg1	cluster					760:766	a 27-kb gene cluster	747:766	a 27-kb gene cluster that was shown to be polycistronic	747:801	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	3	9	theme	gene	755:758	arg1	polycistronic					789:801	polycistronic	789:801	polycistronic	789:801	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	8	10	theme	T.	1909:1910	arg1	immunogenicity					1932:1945	T. forsythia's overall immunogenicity	1909:1945	T. forsythia's overall immunogenicity	1909:1945	Finally, we investigated whether differential cell surface glycosylation impacts T. forsythia's overall immunogenicity.					
30210478	1	11	attach	linked	326:331	arg2	decasaccharide					286:299	a unique, complex decasaccharide	268:299	a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins	268:405	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	1	11	attach	linked	326:331	arg1	layer					374:378	the bacterium's abundant surface (S-) layer	336:378	the bacterium's abundant surface (S-) layer	336:378	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	1	11	attach	linked	326:331	arg1	proteins					398:405	other proteins	392:405	other proteins	392:405	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	11	12	theme	forsythia	2435:2443	arg1	strains					2445:2451	pathogenic T. forsythia strains	2421:2451	pathogenic T. forsythia strains	2421:2451	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	11	13	theme	strains	2445:2451	arg1	O-glycosylation					2388:2402	complex protein O-glycosylation	2372:2402	complex protein O-glycosylation	2372:2402	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	11	13	theme	strains	2445:2451	arg1	hallmark					2409:2416	a hallmark	2407:2416	a hallmark of pathogenic T. forsythia strains	2407:2451	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	6	14	theme	forsythia	1501:1509	arg1	strains					1511:1517	six T. forsythia strains	1494:1517	six T. forsythia strains	1494:1517	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	1	15	theme	pathogen	209:216	arg1	forsythia					229:237	the oral pathogen Tannerella forsythia	200:237	the oral pathogen Tannerella forsythia	200:237	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	5	16	dep	Bacteroidetes	1245:1257	arg1	phylum					1259:1264	phylum	1259:1264	phylum	1259:1264	The core was previously proposed to be conserved within the Bacteroidetes phylum, to which T. forsythia is affiliated, and its biosynthesis is encoded elsewhere on the bacterial genome.					
30210478	9	17	theme	cytokines	1975:1983	arg1	Release					1948:1954	Release	1948:1954	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain	1948:2079	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	1	18	theme	forsythia	229:237	arg1	surface					189:195	The cell surface	180:195	The cell surface of the oral pathogen Tannerella forsythia	180:237	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	9	19	theme	dendritic	1988:1996	arg1	cells					1998:2002	dendritic cells	1988:2002	dendritic cells (DCs)	1988:2008	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	9	19	theme	dendritic	1988:1996	arg1	DCs					2005:2007	DCs	2005:2007	DCs	2005:2007	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	0	20	theme	Immunological	152:164	arg1	Implications					166:177	Immunological Implications	152:177	Immunological Implications	152:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	11	21	theme	pathogenic	2421:2430	arg1	strains					2445:2451	pathogenic T. forsythia strains	2421:2451	pathogenic T. forsythia strains	2421:2451	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	6	22	theme	organization	1578:1589	arg1	clusters					1549:1556	gene clusters	1544:1556	gene clusters of similar size and organization as found in the ATCC 43037 type strain	1544:1628	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	7	23	theme	gene	1743:1746	arg1	composition					1748:1758	a different gene composition	1731:1758	a different gene composition lacking most of the genes commonly found in the pathogenic strains	1731:1825	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	10	24	theme	glycan	2271:2276	arg1	portion					2278:2284	the T. forsythia-specific glycan portion	2245:2284	the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response	2245:2352	This revealed that the O-glycan is pivotal to modulating DC effector functions, with the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response.					
30210478	3	25	theme	pathway	642:648	arg1	components					650:659	pathway components	642:659	pathway components	642:659	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	6	26	theme	size	1569:1572	arg1	clusters					1549:1556	gene clusters	1544:1556	gene clusters of similar size and organization as found in the ATCC 43037 type strain	1544:1628	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	1	27	dep	unique	270:275	arg1	complex					278:284	complex	278:284	complex	278:284	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	0	28	theme	Species-Specific	59:74	arg1	Cluster					39:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	0	28	theme	Species-Specific	59:74	arg1	Glycan					76:81	the Species-Specific Glycan	55:81	the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications	55:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	6	29	theme	O-glycosylation	1413:1427	arg1	prevalence					1391:1400	the prevalence	1387:1400	the prevalence of protein O-glycosylation among Tannerella sp.	1387:1448	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	7	30	theme	isolate	1716:1722	arg1	genome					1663:1668	the genome	1659:1668	the genome of a periodontal health-associated Tannerella isolate	1659:1722	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	4	31	theme	O-glycans	1000:1008	arg1	spectrometry					963:974	mass spectrometry	958:974	mass spectrometry of the protein-released O-glycans	958:1008	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	4	32	theme	pentasaccharide	1163:1177	arg1	core					1179:1182	a pentasaccharide core	1161:1182	a pentasaccharide core	1161:1182	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	1	33	theme	surface	361:367	arg1	layer					374:378	the bacterium's abundant surface (S-) layer	336:378	the bacterium's abundant surface (S-) layer	336:378	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	7	34	theme	health-associated	1687:1703	arg1	isolate					1716:1722	a periodontal health-associated Tannerella isolate	1673:1722	a periodontal health-associated Tannerella isolate	1673:1722	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	11	35	theme	novel	2507:2511	arg1	antimicrobials					2513:2526	novel antimicrobials	2507:2526	novel antimicrobials against periodontitis	2507:2548	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	9	36	theme	defined	2032:2038	arg1	mutants					2054:2060	defined Gtf-deficient mutants	2032:2060	defined Gtf-deficient mutants of the type strain	2032:2079	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	0	37	theme	Oral	90:93	arg1	forsythia					115:123	the Oral Pathogen Tannerella forsythia	86:123	the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications	86:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	1	38	theme	S-	370:371	arg1	layer					374:378	the bacterium's abundant surface (S-) layer	336:378	the bacterium's abundant surface (S-) layer	336:378	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	0	39	theme	Tannerella	104:113	arg1	forsythia					115:123	the Oral Pathogen Tannerella forsythia	86:123	the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications	86:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	6	40	theme	43037	1612:1616	arg1	strain					1623:1628	the ATCC 43037 type strain	1603:1628	the ATCC 43037 type strain	1603:1628	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	4	41	theme	gene	1028:1031	arg1	part					1070:1073	the species-specific part	1049:1073	the species-specific part of the T. forsythia ATCC 43037 decasaccharide	1049:1119	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	4	41	theme	gene	1028:1031	arg1	cluster					1033:1039	the gene cluster	1024:1039	the gene cluster	1024:1039	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	9	42	theme	strain	2074:2079	arg1	mutants					2054:2060	defined Gtf-deficient mutants	2032:2060	defined Gtf-deficient mutants of the type strain	2032:2079	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	2	43	theme	S-layer	412:418	arg1	glycoproteins					420:432	The S-layer glycoproteins	408:432	The S-layer glycoproteins	408:432	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	2	43	theme	S-layer	412:418	arg1	factors					448:454	virulence factors	438:454	virulence factors of T. forsythia	438:470	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	0	44	theme	Protein	10:16	arg1	Cluster					39:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	0	44	theme	Protein	10:16	arg1	Glycan					76:81	the Species-Specific Glycan	55:81	the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications	55:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	7	45	theme	corresponding	1635:1647	arg1	region					1649:1654	The corresponding region	1631:1654	The corresponding region in the genome of a periodontal health-associated Tannerella isolate	1631:1722	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	4	46	theme	gene	812:815	arg1	approach					826:833	a gene deletion approach	810:833	a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster	810:935	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	0	47	theme	Gene	34:37	arg1	Cluster					39:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	0	47	theme	Gene	34:37	arg1	Glycan					76:81	the Species-Specific Glycan	55:81	the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications	55:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	7	48	located	found	1795:1799	arg1	strains					1819:1825	the pathogenic strains	1804:1825	the pathogenic strains	1804:1825	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	7	48	located	found	1795:1799	arg2	genes					1780:1784	the genes	1776:1784	the genes commonly found in the pathogenic strains	1776:1825	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	2	49	theme	T.	459:460	arg1	forsythia					462:470	T. forsythia	459:470	T. forsythia	459:470	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	4	50	theme	T.	1082:1083	arg1	ATCC					1095:1098	the T. forsythia ATCC 43037 decasaccharide	1078:1119	the T. forsythia ATCC 43037 decasaccharide	1078:1119	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	9	51	theme	cell-priming	2106:2117	arg1	post-stimulation					2129:2144	their T cell-priming potential post-stimulation	2098:2144	their T cell-priming potential post-stimulation	2098:2144	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	4	52	theme	ATCC	1095:1098	arg1	part					1070:1073	the species-specific part	1049:1073	the species-specific part of the T. forsythia ATCC 43037 decasaccharide	1049:1119	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	4	52	theme	ATCC	1095:1098	arg1	cluster					1033:1039	the gene cluster	1024:1039	the gene cluster	1024:1039	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	5	53	theme	bacterial	1353:1361	arg1	genome					1363:1368	the bacterial genome	1349:1368	the bacterial genome	1349:1368	The core was previously proposed to be conserved within the Bacteroidetes phylum, to which T. forsythia is affiliated, and its biosynthesis is encoded elsewhere on the bacterial genome.					
30210478	6	54	theme	genome	1474:1479	arg1	sequences					1481:1489	the publicly available genome sequences	1451:1489	the publicly available genome sequences of six T. forsythia strains	1451:1517	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	11	55	theme	T.	2432:2433	arg1	strains					2445:2451	pathogenic T. forsythia strains	2421:2451	pathogenic T. forsythia strains	2421:2451	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	4	56	theme	species-specific	1053:1068	arg1	part					1070:1073	the species-specific part	1049:1073	the species-specific part of the T. forsythia ATCC 43037 decasaccharide	1049:1119	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	4	56	theme	species-specific	1053:1068	arg1	cluster					1033:1039	the gene cluster	1024:1039	the gene cluster	1024:1039	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	3	57	theme	43037	718:722	arg1	strain					729:734	the ATCC 43037 type strain	709:734	the ATCC 43037 type strain	709:734	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	8	58	theme	differential	1861:1872	arg1	glycosylation					1887:1899	differential cell surface glycosylation	1861:1899	differential cell surface glycosylation	1861:1899	Finally, we investigated whether differential cell surface glycosylation impacts T. forsythia's overall immunogenicity.					
30210478	11	59	theme	protein	2380:2386	arg1	O-glycosylation					2388:2402	complex protein O-glycosylation	2372:2402	complex protein O-glycosylation	2372:2402	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	11	59	theme	protein	2380:2386	arg1	hallmark					2409:2416	a hallmark	2407:2416	a hallmark of pathogenic T. forsythia strains	2407:2451	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	8	60	theme	surface	1879:1885	arg1	glycosylation					1887:1899	differential cell surface glycosylation	1861:1899	differential cell surface glycosylation	1861:1899	Finally, we investigated whether differential cell surface glycosylation impacts T. forsythia's overall immunogenicity.					
30210478	10	61	theme	DC	2217:2218	arg1	functions					2229:2237	DC effector functions	2217:2237	DC effector functions	2217:2237	This revealed that the O-glycan is pivotal to modulating DC effector functions, with the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response.					
30210478	1	62	theme	cell	184:187	arg1	surface					189:195	The cell surface	180:195	The cell surface of the oral pathogen Tannerella forsythia	180:237	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	3	63	theme	strain	729:734	arg1	sequence					697:704	the genome sequence	686:704	the genome sequence of the ATCC 43037 type strain	686:734	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	4	64	theme	predicted	847:855	arg1	Gtfs					879:882	Gtfs	879:882	Gtfs	879:882	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	4	64	theme	predicted	847:855	arg1	glycosyltransferases					857:876	predicted glycosyltransferases	847:876	predicted glycosyltransferases (Gtfs)	847:883	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	9	65	theme	T	2104:2104	arg1	post-stimulation					2129:2144	their T cell-priming potential post-stimulation	2098:2144	their T cell-priming potential post-stimulation	2098:2144	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	4	66	theme	decasaccharide	1106:1119	arg1	ATCC					1095:1098	the T. forsythia ATCC 43037 decasaccharide	1078:1119	the T. forsythia ATCC 43037 decasaccharide	1078:1119	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	0	67	theme	O-Glycan	126:133	arg1	Biosynthesis					135:146	O-Glycan Biosynthesis	126:146	O-Glycan Biosynthesis	126:146	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	6	68	theme	strains	1511:1517	arg1	sequences					1481:1489	the publicly available genome sequences	1451:1489	the publicly available genome sequences of six T. forsythia strains	1451:1517	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	1	69	theme	oral	204:207	arg1	forsythia					229:237	the oral pathogen Tannerella forsythia	200:237	the oral pathogen Tannerella forsythia	200:237	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	3	70	theme	protein	581:587	arg1	pathway					605:611	the protein O-glycosylation pathway	577:611	the protein O-glycosylation pathway	577:611	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	4	71	theme	gene	924:927	arg1	cluster					929:935	this gene cluster	919:935	this gene cluster	919:935	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	9	72	theme	proinflammatory	1959:1973	arg1	cytokines					1975:1983	proinflammatory cytokines	1959:1983	proinflammatory cytokines	1959:1983	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	3	73	theme	27-kb	749:753	arg1	cluster					760:766	a 27-kb gene cluster	747:766	a 27-kb gene cluster that was shown to be polycistronic	747:801	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	3	73	theme	27-kb	749:753	arg1	polycistronic					789:801	polycistronic	789:801	polycistronic	789:801	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	6	74	theme	T.	1498:1499	arg1	strains					1511:1517	six T. forsythia strains	1494:1517	six T. forsythia strains	1494:1517	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	1	75	theme	Tannerella	218:227	arg1	forsythia					229:237	the oral pathogen Tannerella forsythia	200:237	the oral pathogen Tannerella forsythia	200:237	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	7	76	from	region	1649:1654	arg1	genome					1663:1668	the genome	1659:1668	the genome of a periodontal health-associated Tannerella isolate	1659:1722	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	1	77	theme	other	392:396	arg1	proteins					398:405	other proteins	392:405	other proteins	392:405	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	3	78	theme	O-glycosylation	589:603	arg1	pathway					605:611	the protein O-glycosylation pathway	577:611	the protein O-glycosylation pathway	577:611	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	8	79	theme	overall	1924:1930	arg1	immunogenicity					1932:1945	T. forsythia's overall immunogenicity	1909:1945	T. forsythia's overall immunogenicity	1909:1945	Finally, we investigated whether differential cell surface glycosylation impacts T. forsythia's overall immunogenicity.					
30210478	10	80	theme	T.	2249:2250	arg1	portion					2278:2284	the T. forsythia-specific glycan portion	2245:2284	the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response	2245:2352	This revealed that the O-glycan is pivotal to modulating DC effector functions, with the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response.					
30210478	6	81	dep	clusters	1549:1556	arg1	found					1594:1598	found	1594:1598	found in the ATCC 43037 type strain	1594:1628	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	9	82	with	stimulation	2015:2025	arg1	mutants					2054:2060	defined Gtf-deficient mutants	2032:2060	defined Gtf-deficient mutants of the type strain	2032:2079	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	6	83	theme	similar	1561:1567	arg1	size					1569:1572	similar size	1561:1572	similar size	1561:1572	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	7	84	theme	different	1733:1741	arg1	composition					1748:1758	a different gene composition	1731:1758	a different gene composition lacking most of the genes commonly found in the pathogenic strains	1731:1825	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	11	85	theme	antimicrobials	2513:2526	arg1	design					2497:2502	the design	2493:2502	the design of novel antimicrobials against periodontitis	2493:2548	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	10	86	theme	effector	2220:2227	arg1	functions					2229:2237	DC effector functions	2217:2237	DC effector functions	2217:2237	This revealed that the O-glycan is pivotal to modulating DC effector functions, with the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response.					
30210478	1	87	theme	abundant	352:359	arg1	layer					374:378	the bacterium's abundant surface (S-) layer	336:378	the bacterium's abundant surface (S-) layer	336:378	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	4	88	theme	protein-released	983:998	arg1	O-glycans					1000:1008	the protein-released O-glycans	979:1008	the protein-released O-glycans	979:1008	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	10	89	theme	forsythia-specific	2252:2269	arg1	portion					2278:2284	the T. forsythia-specific glycan portion	2245:2284	the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response	2245:2352	This revealed that the O-glycan is pivotal to modulating DC effector functions, with the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response.					
30210478	0	90	theme	Pathogen	95:102	arg1	forsythia					115:123	the Oral Pathogen Tannerella forsythia	86:123	the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications	86:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	0	91	dep	forsythia	115:123	arg1	Biosynthesis					135:146	O-Glycan Biosynthesis	126:146	O-Glycan Biosynthesis	126:146	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	0	91	dep	forsythia	115:123	arg1	Implications					166:177	Immunological Implications	152:177	Immunological Implications	152:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	7	92	theme	Tannerella	1705:1714	arg1	isolate					1716:1722	a periodontal health-associated Tannerella isolate	1673:1722	a periodontal health-associated Tannerella isolate	1673:1722	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	9	93	theme	Gtf-deficient	2040:2052	arg1	mutants					2054:2060	defined Gtf-deficient mutants	2032:2060	defined Gtf-deficient mutants of the type strain	2032:2079	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	0	94	theme	forsythia	115:123	arg1	Cluster					39:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	0	94	theme	forsythia	115:123	arg1	Glycan					76:81	the Species-Specific Glycan	55:81	the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications	55:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	1	95	gly	glycosylated	250:261	arg1	surface					189:195	The cell surface	180:195	The cell surface of the oral pathogen Tannerella forsythia	180:237	The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins.					
30210478	7	96	theme	periodontal	1675:1685	arg1	isolate					1716:1722	a periodontal health-associated Tannerella isolate	1673:1722	a periodontal health-associated Tannerella isolate	1673:1722	The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains.					
30210478	4	97	theme	mass	958:961	arg1	spectrometry					963:974	mass spectrometry	958:974	mass spectrometry of the protein-released O-glycans	958:1008	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	0	98	theme	General	2:8	arg1	Cluster					39:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	0	98	theme	General	2:8	arg1	Glycan					76:81	the Species-Specific Glycan	55:81	the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications	55:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	6	99	theme	ATCC	1607:1610	arg1	strain					1623:1628	the ATCC 43037 type strain	1603:1628	the ATCC 43037 type strain	1603:1628	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	2	100	theme	virulence	438:446	arg1	glycoproteins					420:432	The S-layer glycoproteins	408:432	The S-layer glycoproteins	408:432	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	2	100	theme	virulence	438:446	arg1	factors					448:454	virulence factors	438:454	virulence factors of T. forsythia	438:470	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	9	101	theme	type	2069:2072	arg1	strain					2074:2079	the type strain	2065:2079	the type strain	2065:2079	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	0	102	theme	O-Glycosylation	18:32	arg1	Cluster					39:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster	0:45	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	0	102	theme	O-Glycosylation	18:32	arg1	Glycan					76:81	the Species-Specific Glycan	55:81	the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications	55:177	A General Protein O-Glycosylation Gene Cluster Encodes the Species-Specific Glycan of the Oral Pathogen Tannerella forsythia: O-Glycan Biosynthesis and Immunological Implications.					
30210478	6	103	theme	type	1618:1621	arg1	strain					1623:1628	the ATCC 43037 type strain	1603:1628	the ATCC 43037 type strain	1603:1628	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	6	104	theme	protein	1405:1411	arg1	O-glycosylation					1413:1427	protein O-glycosylation	1405:1427	protein O-glycosylation	1405:1427	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30210478	2	105	theme	forsythia	462:470	arg1	glycoproteins					420:432	The S-layer glycoproteins	408:432	The S-layer glycoproteins	408:432	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	2	105	theme	forsythia	462:470	arg1	factors					448:454	virulence factors	438:454	virulence factors of T. forsythia	438:470	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	2	106	theme	protein	499:505	arg1	O-glycosylation					507:521	protein O-glycosylation	499:521	protein O-glycosylation	499:521	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	3	107	theme	genome	690:695	arg1	sequence					697:704	the genome sequence	686:704	the genome sequence of the ATCC 43037 type strain	686:734	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	4	108	theme	deletion	817:824	arg1	approach					826:833	a gene deletion approach	810:833	a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster	810:935	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	11	109	theme	complex	2372:2378	arg1	O-glycosylation					2388:2402	complex protein O-glycosylation	2372:2402	complex protein O-glycosylation	2372:2402	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	11	109	theme	complex	2372:2378	arg1	hallmark					2409:2416	a hallmark	2407:2416	a hallmark of pathogenic T. forsythia strains	2407:2451	We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.					
30210478	9	110	theme	potential	2119:2127	arg1	post-stimulation					2129:2144	their T cell-priming potential post-stimulation	2098:2144	their T cell-priming potential post-stimulation	2098:2144	Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored.					
30210478	2	111	gly	glycoproteins	420:432	arg1	glycoproteins					420:432	The S-layer glycoproteins	408:432	The S-layer glycoproteins	408:432	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	2	111	gly	glycoproteins	420:432	arg1	factors					448:454	virulence factors	438:454	virulence factors of T. forsythia	438:470	The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity.					
30210478	4	112	theme	forsythia	1085:1093	arg1	ATCC					1095:1098	the T. forsythia ATCC 43037 decasaccharide	1078:1119	the T. forsythia ATCC 43037 decasaccharide	1078:1119	Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core.					
30210478	3	113	theme	ATCC	713:716	arg1	strain					729:734	the ATCC 43037 type strain	709:734	the ATCC 43037 type strain	709:734	To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic.					
30210478	10	114	with	pivotal	2195:2201	arg1	portion					2278:2284	the T. forsythia-specific glycan portion	2245:2284	the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response	2245:2352	This revealed that the O-glycan is pivotal to modulating DC effector functions, with the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response.					
30210478	6	115	theme	available	1464:1472	arg1	sequences					1481:1489	the publicly available genome sequences	1451:1489	the publicly available genome sequences of six T. forsythia strains	1451:1517	Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain.					
30080634	5	0	theme	common	737:742	arg1	plants					759:764	common bean composite plants	737:764	common bean composite plants to knock-down PvTRX1h expression	737:797	Through a reverse genetics approach, we generated common bean composite plants to knock-down PvTRX1h expression.					
30080634	2	1	from	bean	427:430	arg1	formation					376:384	the formation	372:384	the formation of nitrogen-fixing nodules in the common bean	372:430	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	4	2	theme	auxin	667:671	arg1	biosynthesis					673:684	auxin biosynthesis	667:684	auxin biosynthesis	667:684	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	4	3	from	role	576:579	arg1	development					651:661	nodule development	644:661	nodule development	644:661	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	4	3	from	role	576:579	arg1	biosynthesis					673:684	auxin biosynthesis	667:684	auxin biosynthesis	667:684	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	7	4	theme	hormone	1095:1101	arg1	concentration					1073:1085	the concentration	1069:1085	the concentration of this hormone	1069:1101	Genes coding for enzymes involved in the synthesis of the indole-3-acetic acid were up-regulated, as was the concentration of this hormone.					
30080634	2	5	theme	legume-rhizobium	336:351	arg1	interaction					353:363	the legume-rhizobium interaction	332:363	the legume-rhizobium interaction	332:363	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	0	6	theme	metabolic	82:90	arg1	fingerprints					92:103	metabolic fingerprints	82:103	metabolic fingerprints	82:103	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	10	7	theme	histone	1465:1471	arg1	modifications					1473:1485	chromatin histone modifications	1455:1485	chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation	1455:1636	Therefore, PvTRX1h likely acts through chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation.					
30080634	5	8	theme	genetics	705:712	arg1	approach					714:721	a reverse genetics approach	695:721	a reverse genetics approach	695:721	Through a reverse genetics approach, we generated common bean composite plants to knock-down PvTRX1h expression.					
30080634	6	9	theme	nodules	874:880	arg1	number					864:869	the number	860:869	the number of nodules per plant	860:890	Here we found that the down-regulation of PvTRX1h increased the number of nodules per plant, but reduced the number of colony-forming units recovered from nodules.					
30080634	10	10	theme	chromatin	1455:1463	arg1	modifications					1473:1485	chromatin histone modifications	1455:1485	chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation	1455:1636	Therefore, PvTRX1h likely acts through chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation.					
30080634	8	11	theme	PvTRX1h	1117:1123	arg1	down-regulation					1125:1139	PvTRX1h down-regulation	1117:1139	PvTRX1h down-regulation	1117:1139	In addition, PvTRX1h down-regulation altered starch accumulation as determined by the number of amyloplasts per nodule.					
30080634	5	12	theme	bean	744:747	arg1	plants					759:764	common bean composite plants	737:764	common bean composite plants to knock-down PvTRX1h expression	737:797	Through a reverse genetics approach, we generated common bean composite plants to knock-down PvTRX1h expression.					
30080634	8	13	theme	starch	1149:1154	arg1	accumulation					1156:1167	starch accumulation	1149:1167	starch accumulation	1149:1167	In addition, PvTRX1h down-regulation altered starch accumulation as determined by the number of amyloplasts per nodule.					
30080634	3	14	from	control	505:511	arg1	bean					541:544	the common bean	530:544	the common bean	530:544	Thus, this study aimed to gain a better understanding of the epigenetic control of nodulation in the common bean.					
30080634	2	15	theme	nitrogen-fixing	389:403	arg1	nodules					405:411	nitrogen-fixing nodules	389:411	nitrogen-fixing nodules in the common bean	389:430	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	10	16	theme	hormone	1599:1605	arg1	levels					1607:1612	hormone levels	1599:1612	hormone levels	1599:1612	Therefore, PvTRX1h likely acts through chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation.					
30080634	10	17	theme	auxin	1502:1506	arg1	network					1518:1524	the auxin signaling network	1498:1524	the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation	1498:1636	Therefore, PvTRX1h likely acts through chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation.					
30080634	3	18	theme	nodulation	516:525	arg1	control					505:511	the epigenetic control	490:511	the epigenetic control of nodulation in the common bean	490:544	Thus, this study aimed to gain a better understanding of the epigenetic control of nodulation in the common bean.					
30080634	4	19	theme	coding	628:633	arg1	gene					635:638	a histone lysine methyltransferase coding gene	593:638	a histone lysine methyltransferase coding gene	593:638	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	4	19	theme	coding	628:633	arg1	PvTRX1h					584:590	PvTRX1h	584:590	PvTRX1h	584:590	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	5	20	theme	PvTRX1h	780:786	arg1	expression					788:797	knock-down PvTRX1h expression	769:797	knock-down PvTRX1h expression	769:797	Through a reverse genetics approach, we generated common bean composite plants to knock-down PvTRX1h expression.					
30080634	3	21	theme	control	505:511	arg1	understanding					473:485	a better understanding	464:485	a better understanding of the epigenetic control of nodulation in the common bean	464:544	Thus, this study aimed to gain a better understanding of the epigenetic control of nodulation in the common bean.					
30080634	2	22	theme	histone	295:301	arg1	methyltransferases					310:327	histone lysine methyltransferases	295:327	histone lysine methyltransferases	295:327	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	10	23	theme	nodule	1563:1568	arg1	number					1570:1575	nodule number	1563:1575	nodule number	1563:1575	Therefore, PvTRX1h likely acts through chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation.					
30080634	10	24	theme	bacterial	1539:1547	arg1	colonization					1549:1560	bacterial colonization	1539:1560	bacterial colonization	1539:1560	Therefore, PvTRX1h likely acts through chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation.					
30080634	9	25	theme	liquid	1259:1264	arg1	spectrometry					1308:1319	direct liquid introduction-electrospray ionization-mass spectrometry	1252:1319	direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS)	1252:1332	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	9	25	theme	liquid	1259:1264	arg1	DLI-ESI-MS					1322:1331	DLI-ESI-MS	1322:1331	DLI-ESI-MS	1322:1331	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	10	26	theme	signaling	1508:1516	arg1	network					1518:1524	the auxin signaling network	1498:1524	the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation	1498:1636	Therefore, PvTRX1h likely acts through chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation.					
30080634	1	27	theme	legume-rhizobium	153:168	arg1	relationship					180:191	The legume-rhizobium symbiotic relationship	149:191	The legume-rhizobium symbiotic relationship	149:191	The legume-rhizobium symbiotic relationship has been widely studied and characterized.					
30080634	9	28	theme	root	1352:1355	arg1	nodules					1357:1363	the root nodules	1348:1363	the root nodules	1348:1363	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	0	29	theme	common	112:117	arg1	bean					119:122	the common bean	108:122	the common bean (Phaseolus vulgaris L.)	108:146	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	3	30	from	understanding	473:485	arg1	bean					541:544	the common bean	530:544	the common bean	530:544	Thus, this study aimed to gain a better understanding of the epigenetic control of nodulation in the common bean.					
30080634	3	31	theme	common	534:539	arg1	bean					541:544	the common bean	530:544	the common bean	530:544	Thus, this study aimed to gain a better understanding of the epigenetic control of nodulation in the common bean.					
30080634	2	32	theme	common	420:425	arg1	bean					427:430	the common bean	416:430	the common bean	416:430	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	0	33	theme	PvTRX1h	19:25	arg1	Down-regulation					0:14	Down-regulation	0:14	Down-regulation of PvTRX1h	0:25	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	6	34	theme	units	934:938	arg1	number					909:914	the number	905:914	the number of colony-forming units recovered from nodules	905:961	Here we found that the down-regulation of PvTRX1h increased the number of nodules per plant, but reduced the number of colony-forming units recovered from nodules.					
30080634	9	35	theme	Metabolic	1224:1232	arg1	fingerprinting					1234:1247	Metabolic fingerprinting	1224:1247	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS)	1224:1332	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	5	36	theme	composite	749:757	arg1	plants					759:764	common bean composite plants	737:764	common bean composite plants to knock-down PvTRX1h expression	737:797	Through a reverse genetics approach, we generated common bean composite plants to knock-down PvTRX1h expression.					
30080634	6	37	theme	colony-forming	919:932	arg1	units					934:938	colony-forming units	919:938	colony-forming units recovered from nodules	919:961	Here we found that the down-regulation of PvTRX1h increased the number of nodules per plant, but reduced the number of colony-forming units recovered from nodules.					
30080634	7	38	theme	indole-3-acetic	1022:1036	arg1	acid					1038:1041	the indole-3-acetic acid	1018:1041	the indole-3-acetic acid	1018:1041	Genes coding for enzymes involved in the synthesis of the indole-3-acetic acid were up-regulated, as was the concentration of this hormone.					
30080634	0	39	from	starch	70:75	arg1	bean					119:122	the common bean	108:122	the common bean (Phaseolus vulgaris L.)	108:146	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	2	40	from	nodules	405:411	arg1	bean					427:430	the common bean	416:430	the common bean	416:430	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	4	41	theme	methyltransferase	610:626	arg1	gene					635:638	a histone lysine methyltransferase coding gene	593:638	a histone lysine methyltransferase coding gene	593:638	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	4	41	theme	methyltransferase	610:626	arg1	PvTRX1h					584:590	PvTRX1h	584:590	PvTRX1h	584:590	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	0	42	theme	nodule	37:42	arg1	number					44:49	nodule number	37:49	nodule number	37:49	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	7	43	theme	acid	1038:1041	arg1	synthesis					1005:1013	the synthesis	1001:1013	the synthesis of the indole-3-acetic acid	1001:1041	Genes coding for enzymes involved in the synthesis of the indole-3-acetic acid were up-regulated, as was the concentration of this hormone.					
30080634	2	44	from	formation	376:384	arg1	bean					427:430	the common bean	416:430	the common bean	416:430	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	2	45	theme	nodules	405:411	arg1	formation					376:384	the formation	372:384	the formation of nitrogen-fixing nodules in the common bean	372:430	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	8	46	theme	amyloplasts	1200:1210	arg1	number					1190:1195	the number	1186:1195	the number of amyloplasts per nodule	1186:1221	In addition, PvTRX1h down-regulation altered starch accumulation as determined by the number of amyloplasts per nodule.					
30080634	5	47	theme	reverse	697:703	arg1	approach					714:721	a reverse genetics approach	695:721	a reverse genetics approach	695:721	Through a reverse genetics approach, we generated common bean composite plants to knock-down PvTRX1h expression.					
30080634	0	48	from	auxin	63:67	arg1	bean					119:122	the common bean	108:122	the common bean (Phaseolus vulgaris L.)	108:146	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	3	49	theme	better	466:471	arg1	understanding					473:485	a better understanding	464:485	a better understanding of the epigenetic control of nodulation in the common bean	464:544	Thus, this study aimed to gain a better understanding of the epigenetic control of nodulation in the common bean.					
30080634	5	50	theme	knock-down	769:778	arg1	expression					788:797	knock-down PvTRX1h expression	769:797	knock-down PvTRX1h expression	769:797	Through a reverse genetics approach, we generated common bean composite plants to knock-down PvTRX1h expression.					
30080634	0	51	theme	Phaseolus	125:133	arg1	vulgaris					135:142	Phaseolus vulgaris	125:142	Phaseolus vulgaris L.	125:145	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	10	52	theme	starch	1578:1583	arg1	accumulation					1585:1596	starch accumulation	1578:1596	starch accumulation	1578:1596	Therefore, PvTRX1h likely acts through chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation.					
30080634	9	53	theme	ionization-mass	1292:1306	arg1	spectrometry					1308:1319	direct liquid introduction-electrospray ionization-mass spectrometry	1252:1319	direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS)	1252:1332	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	9	53	theme	ionization-mass	1292:1306	arg1	DLI-ESI-MS					1322:1331	DLI-ESI-MS	1322:1331	DLI-ESI-MS	1322:1331	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	9	54	theme	PvTRX1h	1391:1397	arg1	down-regulation					1399:1413	PvTRX1h down-regulation	1391:1413	PvTRX1h down-regulation	1391:1413	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	0	55	from	fingerprints	92:103	arg1	bean					119:122	the common bean	108:122	the common bean (Phaseolus vulgaris L.)	108:146	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	2	56	theme	little	245:250	arg1	information					252:262	little information	245:262	little information	245:262	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	4	57	theme	nodule	644:649	arg1	development					651:661	nodule development	644:661	nodule development	644:661	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	0	58	dep	vulgaris	135:142	arg1	L					144:144	L	144:144	Phaseolus vulgaris L.	125:145	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	3	59	from	bean	541:544	arg1	understanding					473:485	a better understanding	464:485	a better understanding of the epigenetic control of nodulation in the common bean	464:544	Thus, this study aimed to gain a better understanding of the epigenetic control of nodulation in the common bean.					
30080634	7	60	dep	concentration	1073:1085	arg1	was					1065:1067	was	1065:1067	was	1065:1067	Genes coding for enzymes involved in the synthesis of the indole-3-acetic acid were up-regulated, as was the concentration of this hormone.					
30080634	7	60	dep	concentration	1073:1085	arg1	as					1062:1063	as	1062:1063	as	1062:1063	Genes coding for enzymes involved in the synthesis of the indole-3-acetic acid were up-regulated, as was the concentration of this hormone.					
30080634	9	61	theme	direct	1252:1257	arg1	spectrometry					1308:1319	direct liquid introduction-electrospray ionization-mass spectrometry	1252:1319	direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS)	1252:1332	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	9	61	theme	direct	1252:1257	arg1	DLI-ESI-MS					1322:1331	DLI-ESI-MS	1322:1331	DLI-ESI-MS	1322:1331	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	2	62	theme	lysine	303:308	arg1	methyltransferases					310:327	histone lysine methyltransferases	295:327	histone lysine methyltransferases	295:327	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	2	63	theme	methyltransferases	310:327	arg1	role					287:290	the role	283:290	the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean	283:430	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	6	64	theme	PvTRX1h	842:848	arg1	down-regulation					823:837	the down-regulation	819:837	the down-regulation of PvTRX1h	819:848	Here we found that the down-regulation of PvTRX1h increased the number of nodules per plant, but reduced the number of colony-forming units recovered from nodules.					
30080634	4	65	theme	lysine	603:608	arg1	gene					635:638	a histone lysine methyltransferase coding gene	593:638	a histone lysine methyltransferase coding gene	593:638	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	4	65	theme	lysine	603:608	arg1	PvTRX1h					584:590	PvTRX1h	584:590	PvTRX1h	584:590	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	1	66	theme	symbiotic	170:178	arg1	relationship					180:191	The legume-rhizobium symbiotic relationship	149:191	The legume-rhizobium symbiotic relationship	149:191	The legume-rhizobium symbiotic relationship has been widely studied and characterized.					
30080634	3	67	theme	epigenetic	494:503	arg1	control					505:511	the epigenetic control	490:511	the epigenetic control of nodulation in the common bean	490:544	Thus, this study aimed to gain a better understanding of the epigenetic control of nodulation in the common bean.					
30080634	9	68	theme	introduction-electrospray	1266:1290	arg1	spectrometry					1308:1319	direct liquid introduction-electrospray ionization-mass spectrometry	1252:1319	direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS)	1252:1332	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	9	68	theme	introduction-electrospray	1266:1290	arg1	DLI-ESI-MS					1322:1331	DLI-ESI-MS	1322:1331	DLI-ESI-MS	1322:1331	Metabolic fingerprinting by direct liquid introduction-electrospray ionization-mass spectrometry (DLI-ESI-MS) revealed that the root nodules were globally affected by PvTRX1h down-regulation.					
30080634	4	69	theme	histone	595:601	arg1	gene					635:638	a histone lysine methyltransferase coding gene	593:638	a histone lysine methyltransferase coding gene	593:638	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	4	69	theme	histone	595:601	arg1	PvTRX1h					584:590	PvTRX1h	584:590	PvTRX1h	584:590	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30080634	10	70	theme	cell	1619:1622	arg1	proliferation					1624:1636	cell proliferation	1619:1636	cell proliferation	1619:1636	Therefore, PvTRX1h likely acts through chromatin histone modifications that alter the auxin signaling network to determine bacterial colonization, nodule number, starch accumulation, hormone levels, and cell proliferation.					
30080634	0	71	dep	bean	119:122	arg1	vulgaris					135:142	Phaseolus vulgaris	125:142	Phaseolus vulgaris L.	125:145	Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.).					
30080634	2	72	from	role	287:290	arg1	interaction					353:363	the legume-rhizobium interaction	332:363	the legume-rhizobium interaction	332:363	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	2	72	from	role	287:290	arg1	formation					376:384	the formation	372:384	the formation of nitrogen-fixing nodules in the common bean	372:430	However, little information is available about the role of histone lysine methyltransferases in the legume-rhizobium interaction and in the formation of nitrogen-fixing nodules in the common bean.					
30080634	4	73	theme	PvTRX1h	584:590	arg1	role					576:579	the role	572:579	the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis	572:684	Specifically, we studied the role of PvTRX1h, a histone lysine methyltransferase coding gene, in nodule development and auxin biosynthesis.					
30040175	2	0	theme	functional	557:566	arg1	properties					579:588	functional mechanical properties	557:588	functional mechanical properties	557:588	In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties.					
30040175	8	1	theme	or	1635:1636	arg1	components					1644:1653	individual hydrogel or fiber components	1615:1653	individual hydrogel or fiber components	1615:1653	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	8	2	theme	fiber-reinforced	1441:1456	arg1	hydrogel					1462:1469	a fiber-reinforced IPN hydrogel	1439:1469	a fiber-reinforced IPN hydrogel	1439:1469	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	5	3	theme	high	942:945	arg1	viability					947:955	high viability	942:955	high viability of MSCs	942:963	Results show high viability of MSCs within the IPN hydrogel, with improved mechanical properties compared to constructs comprised of individual components.					
30040175	8	4	dep	enhanced	1541:1548	arg1	complex					1551:1557	complex	1551:1557	complex	1551:1557	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	5	5	theme	improved	995:1002	arg1	properties					1015:1024	improved mechanical properties	995:1024	improved mechanical properties compared to constructs comprised of individual components	995:1082	Results show high viability of MSCs within the IPN hydrogel, with improved mechanical properties compared to constructs comprised of individual components.					
30040175	5	6	theme	MSCs	960:963	arg1	viability					947:955	high viability	942:955	high viability of MSCs	942:963	Results show high viability of MSCs within the IPN hydrogel, with improved mechanical properties compared to constructs comprised of individual components.					
30040175	7	7	theme	resulting	1186:1194	arg1	hydrogels					1213:1221	The resulting fiber-reinforced hydrogels	1182:1221	The resulting fiber-reinforced hydrogels	1182:1221	The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function.					
30040175	1	8	theme	cartilage	172:180	arg1	engineering					189:199	cartilage tissue engineering	172:199	cartilage tissue engineering	172:199	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	0	9	theme	3D	108:109	arg1	Scaffold					117:124	a 3D Woven Scaffold	106:124	a 3D Woven Scaffold	106:124	Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.					
30040175	8	10	theme	hydrogel	1626:1633	arg1	components					1644:1653	individual hydrogel or fiber components	1615:1653	individual hydrogel or fiber components	1615:1653	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	3	11	theme	polymer	624:630	arg1	hydrogels					646:654	interpenetrating polymer network (IPN) hydrogels	607:654	interpenetrating polymer network (IPN) hydrogels	607:654	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	1	12	theme	embedded	289:296	arg1	cells					298:302	embedded cells	289:302	embedded cells	289:302	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	7	13	theme	articular	1302:1310	arg1	cartilage					1312:1320	native articular cartilage	1295:1320	native articular cartilage	1295:1320	The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function.					
30040175	1	14	theme	native	406:411	arg1	joint					413:417	the native joint	402:417	the native joint	402:417	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	4	15	theme	agarose	830:836	arg1	hydrogel					868:875	an agarose and poly(ethylene) glycol IPN hydrogel	827:875	an agarose and poly(ethylene) glycol IPN hydrogel	827:875	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	7	16	theme	local	1341:1345	arg1	microenvironment					1347:1362	a local microenvironment	1339:1362	a local microenvironment that supports cellular viability and function	1339:1408	The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function.					
30040175	3	17	theme	network	632:638	arg1	hydrogels					646:654	interpenetrating polymer network (IPN) hydrogels	607:654	interpenetrating polymer network (IPN) hydrogels	607:654	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	1	18	theme	cells	298:302	arg1	growth					279:284	growth	279:284	growth of embedded cells	279:302	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	4	19	theme	mesenchymal	898:908	arg1	MSCs					922:925	MSCs	922:925	MSCs	922:925	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	4	19	theme	mesenchymal	898:908	arg1	cells					915:919	human mesenchymal stem cells	892:919	human mesenchymal stem cells (MSCs)	892:926	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	1	20	theme	tissue	182:187	arg1	engineering					189:199	cartilage tissue engineering	172:199	cartilage tissue engineering	172:199	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	0	21	theme	Woven	111:115	arg1	Scaffold					117:124	a 3D Woven Scaffold	106:124	a 3D Woven Scaffold	106:124	Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.					
30040175	3	22	theme	cell	792:795	arg1	encapsulation					797:809	cell encapsulation	792:809	cell encapsulation	792:809	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	4	23	theme	human	892:896	arg1	MSCs					922:925	MSCs	922:925	MSCs	922:925	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	4	23	theme	human	892:896	arg1	cells					915:919	human mesenchymal stem cells	892:919	human mesenchymal stem cells (MSCs)	892:926	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	3	24	theme	interpenetrating	607:622	arg1	hydrogels					646:654	interpenetrating polymer network (IPN) hydrogels	607:654	interpenetrating polymer network (IPN) hydrogels	607:654	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	2	25	theme	hydrogels-with	435:448	arg1	content					461:467	hydrogels-with high water content	435:467	hydrogels-with high water content	435:467	In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties.					
30040175	0	26	theme	Cellularized	10:21	arg1	Structures					23:32	Composite Cellularized Structures	0:32	Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel	0:90	Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.					
30040175	5	27	theme	IPN	976:978	arg1	hydrogel					980:987	the IPN hydrogel	972:987	the IPN hydrogel	972:987	Results show high viability of MSCs within the IPN hydrogel, with improved mechanical properties compared to constructs comprised of individual components.					
30040175	7	28	theme	cellular	1378:1385	arg1	viability					1387:1395	cellular viability	1378:1395	cellular viability	1378:1395	The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function.					
30040175	0	29	theme	Composite	0:8	arg1	Structures					23:32	Composite Cellularized Structures	0:32	Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel	0:90	Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.					
30040175	4	30	theme	stem	910:913	arg1	MSCs					922:925	MSCs	922:925	MSCs	922:925	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	4	30	theme	stem	910:913	arg1	cells					915:919	human mesenchymal stem cells	892:919	human mesenchymal stem cells (MSCs)	892:926	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	8	31	theme	stem	1483:1486	arg1	chondrogenesis					1493:1506	stem cell chondrogenesis	1483:1506	stem cell chondrogenesis	1483:1506	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	7	32	theme	macroscopic	1242:1252	arg1	properties					1265:1274	functional macroscopic mechanical properties	1231:1274	functional macroscopic mechanical properties mimicking those of native articular cartilage	1231:1320	The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function.					
30040175	5	33	theme	individual	1062:1071	arg1	components					1073:1082	individual components	1062:1082	individual components	1062:1082	Results show high viability of MSCs within the IPN hydrogel, with improved mechanical properties compared to constructs comprised of individual components.					
30040175	1	34	theme	controlling	212:222	arg1	architecture					224:235	controlling architecture	212:235	controlling architecture of newly formed tissue	212:258	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	2	35	theme	transport	483:491	arg1	properties-while					493:508	desirable transport properties-while	473:508	desirable transport properties-while highly conducive to chondrogenesis	473:543	In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties.					
30040175	8	36	theme	multiple	1584:1591	arg1	scales					1593:1598	multiple scales	1584:1598	multiple scales	1584:1598	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	3	37	theme	IPN	641:643	arg1	hydrogels					646:654	interpenetrating polymer network (IPN) hydrogels	607:654	interpenetrating polymer network (IPN) hydrogels	607:654	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	6	38	theme	woven	1165:1169	arg1	structure					1171:1179	a 3D woven structure	1160:1179	a 3D woven structure	1160:1179	These properties are further strengthened by integrating the hydrogel with a 3D woven structure.					
30040175	1	39	theme	functional	333:342	arg1	properties					344:353	functional properties	333:353	functional properties	333:353	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	8	40	theme	individual	1615:1624	arg1	components					1644:1653	individual hydrogel or fiber components	1615:1653	individual hydrogel or fiber components	1615:1653	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	3	41	theme	mechanical	668:677	arg1	toughness					679:687	mechanical toughness	668:687	mechanical toughness	668:687	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	7	42	theme	fiber-reinforced	1196:1211	arg1	hydrogels					1213:1221	The resulting fiber-reinforced hydrogels	1182:1221	The resulting fiber-reinforced hydrogels	1182:1221	The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function.					
30040175	2	43	theme	high	450:453	arg1	content					461:467	hydrogels-with high water content	435:467	hydrogels-with high water content	435:467	In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties.					
30040175	7	44	theme	functional	1231:1240	arg1	properties					1265:1274	functional macroscopic mechanical properties	1231:1274	functional macroscopic mechanical properties mimicking those of native articular cartilage	1231:1320	The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function.					
30040175	3	45	theme	individual	715:724	arg1	components					726:735	individual components	715:735	individual components	715:735	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	8	46	from	scales	1593:1598	arg1	properties					1570:1579	significantly enhanced, complex mechanical properties	1527:1579	significantly enhanced, complex mechanical properties at multiple scales	1527:1598	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	2	47	theme	water	455:459	arg1	content					461:467	hydrogels-with high water content	435:467	hydrogels-with high water content	435:467	In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties.					
30040175	0	48	theme	Polymer	67:73	arg1	Hydrogel					83:90	an Interpenetrating Polymer Network Hydrogel	47:90	an Interpenetrating Polymer Network Hydrogel	47:90	Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.					
30040175	7	49	theme	native	1295:1300	arg1	cartilage					1312:1320	native articular cartilage	1295:1320	native articular cartilage	1295:1320	The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function.					
30040175	8	50	theme	cell	1488:1491	arg1	chondrogenesis					1493:1506	stem cell chondrogenesis	1483:1506	stem cell chondrogenesis	1483:1506	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	2	51	theme	mechanical	568:577	arg1	properties					579:588	functional mechanical properties	557:588	functional mechanical properties	557:588	In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties.					
30040175	5	52	theme	mechanical	1004:1013	arg1	properties					1015:1024	improved mechanical properties	995:1024	improved mechanical properties compared to constructs comprised of individual components	995:1082	Results show high viability of MSCs within the IPN hydrogel, with improved mechanical properties compared to constructs comprised of individual components.					
30040175	0	53	theme	Interpenetrating	50:65	arg1	Hydrogel					83:90	an Interpenetrating Polymer Network Hydrogel	47:90	an Interpenetrating Polymer Network Hydrogel	47:90	Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.					
30040175	8	54	theme	enhanced	1541:1548	arg1	properties					1570:1579	significantly enhanced, complex mechanical properties	1527:1579	significantly enhanced, complex mechanical properties at multiple scales	1527:1598	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	8	55	theme	fiber	1638:1642	arg1	components					1644:1653	individual hydrogel or fiber components	1615:1653	individual hydrogel or fiber components	1615:1653	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	3	56	theme	IPN	752:754	arg1	materials					756:764	many IPN materials	747:764	many IPN materials	747:764	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	7	57	theme	mechanical	1254:1263	arg1	properties					1265:1274	functional macroscopic mechanical properties	1231:1274	functional macroscopic mechanical properties mimicking those of native articular cartilage	1231:1320	The resulting fiber-reinforced hydrogels display functional macroscopic mechanical properties mimicking those of native articular cartilage, while providing a local microenvironment that supports cellular viability and function.					
30040175	1	58	theme	Biomaterial	127:137	arg1	scaffolds					139:147	Biomaterial scaffolds	127:147	Biomaterial scaffolds	127:147	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	8	59	theme	mechanical	1559:1568	arg1	properties					1570:1579	significantly enhanced, complex mechanical properties	1527:1579	significantly enhanced, complex mechanical properties at multiple scales	1527:1598	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	1	60	theme	formed	246:251	arg1	tissue					253:258	newly formed tissue	240:258	newly formed tissue	240:258	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	4	61	theme	poly	842:845	arg1	glycol					857:862	poly(ethylene) glycol	842:862	poly(ethylene) glycol	842:862	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	6	62	with	hydrogel	1146:1153	arg1	structure					1171:1179	a 3D woven structure	1160:1179	a 3D woven structure	1160:1179	These properties are further strengthened by integrating the hydrogel with a 3D woven structure.					
30040175	4	63	theme	ethylene	847:854	arg1	glycol					857:862	poly(ethylene) glycol	842:862	poly(ethylene) glycol	842:862	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	6	64	theme	3D	1162:1163	arg1	structure					1171:1179	a 3D woven structure	1160:1179	a 3D woven structure	1160:1179	These properties are further strengthened by integrating the hydrogel with a 3D woven structure.					
30040175	8	65	theme	IPN	1458:1460	arg1	hydrogel					1462:1469	a fiber-reinforced IPN hydrogel	1439:1469	a fiber-reinforced IPN hydrogel	1439:1469	These findings suggest that a fiber-reinforced IPN hydrogel can support stem cell chondrogenesis while allowing for significantly enhanced, complex mechanical properties at multiple scales as compared to individual hydrogel or fiber components.					
30040175	1	66	theme	tissue	253:258	arg1	architecture					224:235	controlling architecture	212:235	controlling architecture of newly formed tissue	212:258	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	3	67	theme	many	747:750	arg1	materials					756:764	many IPN materials	747:764	many IPN materials	747:764	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	4	68	theme	IPN	864:866	arg1	hydrogel					868:875	an agarose and poly(ethylene) glycol IPN hydrogel	827:875	an agarose and poly(ethylene) glycol IPN hydrogel	827:875	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	2	69	theme	desirable	473:481	arg1	properties-while					493:508	desirable transport properties-while	473:508	desirable transport properties-while highly conducive to chondrogenesis	473:543	In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties.					
30040175	1	70	theme	multiple	154:161	arg1	roles					163:167	multiple roles	154:167	multiple roles	154:167	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	3	71	dep	provide	660:666	arg1	biocompatible					774:786	biocompatible	774:786	biocompatible	774:786	In this regard, interpenetrating polymer network (IPN) hydrogels can provide mechanical toughness greatly exceeding that of individual components; however, many IPN materials are not biocompatible for cell encapsulation.					
30040175	0	72	theme	Network	75:81	arg1	Hydrogel					83:90	an Interpenetrating Polymer Network Hydrogel	47:90	an Interpenetrating Polymer Network Hydrogel	47:90	Composite Cellularized Structures Created from an Interpenetrating Polymer Network Hydrogel Reinforced by a 3D Woven Scaffold.					
30040175	1	73	theme	mechanical	372:381	arg1	environment					383:393	the mechanical environment	368:393	the mechanical environment within the native joint	368:417	Biomaterial scaffolds play multiple roles in cartilage tissue engineering, including controlling architecture of newly formed tissue while facilitating growth of embedded cells and simultaneously providing functional properties to withstand the mechanical environment within the native joint.					
30040175	4	74	theme	glycol	857:862	arg1	hydrogel					868:875	an agarose and poly(ethylene) glycol IPN hydrogel	827:875	an agarose and poly(ethylene) glycol IPN hydrogel	827:875	In this study, an agarose and poly(ethylene) glycol IPN hydrogel is seeded with human mesenchymal stem cells (MSCs).					
30040175	2	75	theme	conducive	517:525	arg1	properties-while					493:508	desirable transport properties-while	473:508	desirable transport properties-while highly conducive to chondrogenesis	473:543	In particular, hydrogels-with high water content and desirable transport properties-while highly conducive to chondrogenesis, often lack functional mechanical properties.					
29575825	0	0	theme	acid	73:76	arg1	composition					78:88	gut microbiota and short chain fatty acid composition	36:88	composition	78:88	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	5	1	dep	bacteria	839:846	arg1	SCFAs					887:891	branched-chain SCFAs	872:891	branched-chain SCFAs (BCFAs)	872:899	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	1	dep	bacteria	839:846	arg1	BCFAs					894:898	BCFAs	894:898	BCFAs	894:898	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	1	dep	bacteria	839:846	arg1	Desulfovibrionaceae					848:866	Desulfovibrionaceae	848:866	Desulfovibrionaceae	848:866	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	4	2	theme	fatty	450:454	arg1	SCFAs					463:467	SCFAs	463:467	SCFAs	463:467	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	4	2	theme	fatty	450:454	arg1	acids					456:460	short chain fatty acids	438:460	short chain fatty acids (SCFAs)	438:468	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	6	3	theme	microbiota	1033:1042	arg1	structure					1005:1013	the structure	1001:1013	the structure of the intestinal microbiota, which may be beneficial for host health	1001:1083	These results suggest that lactulose intervention effectively increased the diversity and improved the structure of the intestinal microbiota, which may be beneficial for host health.					
29575825	5	4	theme	mucin-degrading	749:763	arg1	Akkermansia					774:784	Akkermansia	774:784	Akkermansia	774:784	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	4	theme	mucin-degrading	749:763	arg1	bacteria					765:772	mucin-degrading bacteria	749:772	mucin-degrading bacteria Akkermansia and Helicobacter;	749:802	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	4	theme	mucin-degrading	749:763	arg1	Helicobacter					790:801	Helicobacter	790:801	Helicobacter	790:801	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	0	5	theme	fatty	67:71	arg1	composition					78:88	gut microbiota and short chain fatty acid composition	36:88	composition	78:88	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	5	6	theme	hydrogen-producing	631:648	arg1	bacteria					650:657	hydrogen-producing bacteria	631:657	hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae	631:742	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	6	theme	hydrogen-producing	631:648	arg1	Bifidobacteriaceae					704:721	Bifidobacteriaceae	704:721	Bifidobacteriaceae	704:721	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	6	theme	hydrogen-producing	631:648	arg1	Prevotellaceae					659:672	Prevotellaceae	659:672	Prevotellaceae	659:672	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	6	theme	hydrogen-producing	631:648	arg1	Lactobacillaceae					727:742	Lactobacillaceae	727:742	Lactobacillaceae	727:742	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	0	7	from	Effect	0:5	arg1	microbiota					40:49	gut microbiota and short chain fatty acid composition	36:88	microbiota	40:49	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	0	7	from	Effect	0:5	arg1	composition					78:88	gut microbiota and short chain fatty acid composition	36:88	composition	78:88	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	6	8	theme	lactulose	929:937	arg1	intervention					939:950	lactulose intervention	929:950	lactulose intervention	929:950	These results suggest that lactulose intervention effectively increased the diversity and improved the structure of the intestinal microbiota, which may be beneficial for host health.					
29575825	3	9	dep	short-term	266:275	arg1	3 week					278:283	3 week	278:283	3 week	278:283	In this study, the effect of short-term (3 week) intervention of lactulose on gut microbiota was investigated.					
29575825	4	10	theme	chain	444:448	arg1	SCFAs					463:467	SCFAs	463:467	SCFAs	463:467	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	4	10	theme	chain	444:448	arg1	acids					456:460	short chain fatty acids	438:460	short chain fatty acids (SCFAs)	438:468	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	1	11	with	connections	135:145	arg1	health					152:157	health	152:157	health	152:157	Gut microbiota have strong connections with health.					
29575825	4	12	theme	mouse	382:386	arg1	feces					388:392	mouse feces	382:392	mouse feces	382:392	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	3	13	from	effect	256:261	arg1	microbiota					319:328	gut microbiota	315:328	gut microbiota	315:328	In this study, the effect of short-term (3 week) intervention of lactulose on gut microbiota was investigated.					
29575825	0	14	theme	mice	102:105	arg1	microbiota					40:49	gut microbiota and short chain fatty acid composition	36:88	microbiota	40:49	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	0	14	theme	mice	102:105	arg1	composition					78:88	gut microbiota and short chain fatty acid composition	36:88	composition	78:88	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	4	15	theme	short	438:442	arg1	SCFAs					463:467	SCFAs	463:467	SCFAs	463:467	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	4	15	theme	short	438:442	arg1	acids					456:460	short chain fatty acids	438:460	short chain fatty acids (SCFAs)	438:468	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	5	16	theme	gut	588:590	arg1	microbiota					592:601	the gut microbiota	584:601	the gut microbiota	584:601	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	0	17	theme	C57BL/6J	93:100	arg1	mice					102:105	C57BL/6J mice	93:105	C57BL/6J mice	93:105	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	5	18	theme	microbiota	592:601	arg1	α-diversity					569:579	the α-diversity	565:579	the α-diversity of the gut microbiota	565:601	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	19	dep	bacteria	650:657	arg1	probiotics					693:702	probiotics	693:702	probiotics	693:702	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	19	dep	bacteria	650:657	arg1	Lactobacillaceae					727:742	Lactobacillaceae	727:742	Lactobacillaceae	727:742	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	19	dep	bacteria	650:657	arg1	Bifidobacteriaceae					704:721	Bifidobacteriaceae	704:721	Bifidobacteriaceae	704:721	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	19	dep	bacteria	650:657	arg1	Prevotellaceae					659:672	Prevotellaceae	659:672	Prevotellaceae	659:672	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	19	dep	bacteria	650:657	arg1	bacteria					650:657	hydrogen-producing bacteria	631:657	hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae	631:742	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	3	20	theme	intervention	286:297	arg1	effect					256:261	the effect	252:261	the effect of short-term (3 week) intervention of lactulose on gut microbiota	252:328	In this study, the effect of short-term (3 week) intervention of lactulose on gut microbiota was investigated.					
29575825	5	21	theme	Lactulose	533:541	arg1	intervention					543:554	Lactulose intervention	533:554	Lactulose intervention	533:554	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	22	theme	harmful	831:837	arg1	bacteria					839:846	harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs)	831:899	harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs)	831:899	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	6	23	theme	intestinal	1022:1031	arg1	beneficial					1058:1067	beneficial	1058:1067	beneficial	1058:1067	These results suggest that lactulose intervention effectively increased the diversity and improved the structure of the intestinal microbiota, which may be beneficial for host health.					
29575825	6	23	theme	intestinal	1022:1031	arg1	microbiota					1033:1042	the intestinal microbiota	1018:1042	the intestinal microbiota	1018:1042	These results suggest that lactulose intervention effectively increased the diversity and improved the structure of the intestinal microbiota, which may be beneficial for host health.					
29575825	0	24	theme	intervention	20:31	arg1	Effect					0:5	Effect	0:5	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.	0:106	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	4	25	theme	Gut	348:350	arg1	microbiota					352:361	Gut microbiota	348:361	Gut microbiota	348:361	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	0	26	theme	lactulose	10:18	arg1	intervention					20:31	lactulose intervention	10:31	lactulose intervention	10:31	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	5	27	theme	branched-chain	872:885	arg1	SCFAs					887:891	branched-chain SCFAs	872:891	branched-chain SCFAs (BCFAs)	872:899	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	27	theme	branched-chain	872:885	arg1	BCFAs					894:898	BCFAs	894:898	BCFAs	894:898	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	0	28	theme	gut	36:38	arg1	microbiota					40:49	gut microbiota and short chain fatty acid composition	36:88	microbiota	40:49	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	5	29	dep	bacteria	765:772	arg1	Akkermansia					774:784	Akkermansia	774:784	Akkermansia	774:784	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	29	dep	bacteria	765:772	arg1	bacteria					765:772	mucin-degrading bacteria	749:772	mucin-degrading bacteria Akkermansia and Helicobacter;	749:802	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	5	29	dep	bacteria	765:772	arg1	Helicobacter					790:801	Helicobacter	790:801	Helicobacter	790:801	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	4	30	theme	high-throughput	406:420	arg1	sequencing					422:431	rRNA high-throughput sequencing	401:431	rRNA high-throughput sequencing	401:431	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	2	31	theme	host	224:227	arg1	health					229:234	host health	224:234	host health	224:234	Lactulose has been shown to regulate gut microbiota and benefit host health.					
29575825	5	32	theme	bacteria	839:846	arg1	abundance					818:826	the abundance	814:826	the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs)	814:899	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	3	33	theme	gut	315:317	arg1	microbiota					319:328	gut microbiota	315:328	gut microbiota	315:328	In this study, the effect of short-term (3 week) intervention of lactulose on gut microbiota was investigated.					
29575825	5	34	theme	bacteria	650:657	arg1	abundance					618:626	the abundance	614:626	the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae	614:742	Lactulose intervention enhanced the α-diversity of the gut microbiota; increased the abundance of hydrogen-producing bacteria Prevotellaceae and Rikenellaceae, probiotics Bifidobacteriaceae and Lactobacillaceae, and mucin-degrading bacteria Akkermansia and Helicobacter; decreased the abundance of harmful bacteria Desulfovibrionaceae and branched-chain SCFAs (BCFAs).					
29575825	0	35	theme	short	55:59	arg1	composition					78:88	gut microbiota and short chain fatty acid composition	36:88	composition	78:88	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	4	36	theme	chromatography-mass	491:509	arg1	GC-MS					525:529	GC-MS	525:529	GC-MS	525:529	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	4	36	theme	chromatography-mass	491:509	arg1	spectrometry					511:522	gas chromatography-mass spectrometry	487:522	gas chromatography-mass spectrometry (GC-MS)	487:530	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	2	37	theme	gut	197:199	arg1	microbiota					201:210	gut microbiota	197:210	gut microbiota	197:210	Lactulose has been shown to regulate gut microbiota and benefit host health.					
29575825	1	38	theme	Gut	108:110	arg1	microbiota					112:121	Gut microbiota	108:121	Gut microbiota	108:121	Gut microbiota have strong connections with health.					
29575825	4	39	theme	gas	487:489	arg1	GC-MS					525:529	GC-MS	525:529	GC-MS	525:529	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	4	39	theme	gas	487:489	arg1	spectrometry					511:522	gas chromatography-mass spectrometry	487:522	gas chromatography-mass spectrometry (GC-MS)	487:530	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	6	40	theme	host	1073:1076	arg1	health					1078:1083	host health	1073:1083	host health	1073:1083	These results suggest that lactulose intervention effectively increased the diversity and improved the structure of the intestinal microbiota, which may be beneficial for host health.					
29575825	3	41	theme	short-term	266:275	arg1	intervention					286:297	short-term (3 week) intervention	266:297	short-term (3 week) intervention of lactulose	266:310	In this study, the effect of short-term (3 week) intervention of lactulose on gut microbiota was investigated.					
29575825	4	42	theme	rRNA	401:404	arg1	sequencing					422:431	rRNA high-throughput sequencing	401:431	rRNA high-throughput sequencing	401:431	Gut microbiota were detected from mouse feces by 16S rRNA high-throughput sequencing, and short chain fatty acids (SCFAs) were detected by gas chromatography-mass spectrometry (GC-MS).					
29575825	0	43	theme	chain	61:65	arg1	composition					78:88	gut microbiota and short chain fatty acid composition	36:88	composition	78:88	Effect of lactulose intervention on gut microbiota and short chain fatty acid composition of C57BL/6J mice.					
29575825	1	44	theme	strong	128:133	arg1	connections					135:145	strong connections	128:145	strong connections with health	128:157	Gut microbiota have strong connections with health.					
29575825	3	45	theme	lactulose	302:310	arg1	intervention					286:297	short-term (3 week) intervention	266:297	short-term (3 week) intervention of lactulose	266:310	In this study, the effect of short-term (3 week) intervention of lactulose on gut microbiota was investigated.					
29575825	1	46	contain	have	123:126	arg1	microbiota					112:121	Gut microbiota	108:121	Gut microbiota	108:121	Gut microbiota have strong connections with health.					
29575825	1	46	contain	have	123:126	arg2	connections					135:145	strong connections	128:145	strong connections with health	128:157	Gut microbiota have strong connections with health.					
31781998	3	0	theme	glycosaminoglycans	704:721	arg1	levels					688:693	the levels	684:693	the levels of total glycosaminoglycans, proteins, and galactose	684:746	In contrast to INH, both preparations did not decrease the levels of total glycosaminoglycans, proteins, and galactose.					
31781998	2	1	theme	acids	622:626	arg1	levels					573:578	the levels	569:578	the levels of sulfated glycosaminoglycans and uronic acids	569:626	Both dextrazide and its liposomal form decreased the levels of sulfated glycosaminoglycans and uronic acids.					
31781998	2	2	theme	sulfated	583:590	arg1	glycosaminoglycans					592:609	sulfated glycosaminoglycans	583:609	sulfated glycosaminoglycans	583:609	Both dextrazide and its liposomal form decreased the levels of sulfated glycosaminoglycans and uronic acids.					
31781998	1	3	theme	oxidized	255:262	arg1	dextrazide					243:252	dextrazide	243:252	dextrazide (oxidized dextran+INH)	243:275	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	1	3	theme	oxidized	255:262	arg1	dextran+INH					264:274	oxidized dextran+INH	255:274	oxidized dextran+INH	255:274	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	4	4	theme	TIMP-2	951:956	arg1	content					958:964	TIMP-2 content	951:964	TIMP-2 content	951:964	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	1	5	theme	mg/kg	330:334	arg1	dose					319:322	INH dose	315:322	INH dose of 14 mg/kg	315:334	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	1	6	theme	postinfection	404:416	arg1	day					418:420	postinfection day 90	404:423	postinfection day 90	404:423	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	0	7	from	Effect	0:5	arg1	Profile					95:101	Component Profile	85:101	Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis	85:175	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	0	8	theme	Component	85:93	arg1	Profile					95:101	Component Profile	85:101	Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis	85:175	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	2	9	theme	glycosaminoglycans	592:609	arg1	levels					573:578	the levels	569:578	the levels of sulfated glycosaminoglycans and uronic acids	569:626	Both dextrazide and its liposomal form decreased the levels of sulfated glycosaminoglycans and uronic acids.					
31781998	1	10	theme	qualitative	433:443	arg1	changes					462:468	qualitative and quantitative changes	433:468	qualitative and quantitative changes	433:468	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	4	11	theme	conditions	1099:1108	arg1	maintenance					1080:1090	maintenance	1080:1090	maintenance	1080:1090	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	4	11	theme	conditions	1099:1108	arg1	degradation					1064:1074	collagen degradation	1055:1074	collagen degradation	1055:1074	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	0	12	theme	Pulmonary	106:114	arg1	Matrix					130:135	Pulmonary Extracellular Matrix	106:135	Pulmonary Extracellular Matrix	106:135	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	4	13	theme	hydroxyproline	1002:1015	arg1	level					988:992	the level	984:992	the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group	984:1153	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	1	14	theme	Intraperitoneal	178:192	arg1	injections					194:203	Intraperitoneal injections	178:203	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90	178:423	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	1	15	dep	dextrazide	303:312	arg1	dose					319:322	INH dose	315:322	INH dose of 14 mg/kg	315:334	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	1	16	theme	quantitative	449:460	arg1	changes					462:468	qualitative and quantitative changes	433:468	qualitative and quantitative changes	433:468	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	4	17	theme	pulmonary	1114:1122	arg1	fibrosis					1124:1131	pulmonary fibrosis	1114:1131	pulmonary fibrosis in mice of this group	1114:1153	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	4	18	dep	fact	785:788	arg1	activated					825:833	activated	825:833	activated MMP	825:837	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	4	18	dep	fact	785:788	arg1	both					790:793	both	790:793	both	790:793	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	4	18	dep	fact	785:788	arg1	increase					852:859	increase	852:859	increase	852:859	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	3	19	theme	proteins	724:731	arg1	levels					688:693	the levels	684:693	the levels of total glycosaminoglycans, proteins, and galactose	684:746	In contrast to INH, both preparations did not decrease the levels of total glycosaminoglycans, proteins, and galactose.					
31781998	4	20	theme	INH	916:918	arg1	injection					903:911	injection	903:911	injection of INH	903:918	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	1	21	theme	isonicotinic	208:219	arg1	hydrazide					226:234	isonicotinic acid hydrazide	208:234	isonicotinic acid hydrazide (INH)	208:240	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	1	21	theme	isonicotinic	208:219	arg1	INH					237:239	INH	237:239	INH	237:239	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	0	22	theme	Delivery	20:27	arg1	Effect					0:5	Effect	0:5	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.	0:176	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	1	23	theme	liposomes	281:289	arg1	injections					194:203	Intraperitoneal injections	178:203	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90	178:423	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	0	24	theme	Matrix	130:135	arg1	Profile					95:101	Component Profile	85:101	Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis	85:175	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	4	25	theme	degradation	1064:1074	arg1	down-regulation					1036:1050	down-regulation	1036:1050	down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group	1036:1153	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	1	26	theme	acid	221:224	arg1	hydrazide					226:234	isonicotinic acid hydrazide	208:234	isonicotinic acid hydrazide (INH)	208:240	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	1	26	theme	acid	221:224	arg1	INH					237:239	INH	237:239	INH	237:239	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	0	27	theme	Liposomal	10:18	arg1	Delivery					20:27	Liposomal Delivery	10:27	Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide	10:80	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	3	28	theme	galactose	738:746	arg1	levels					688:693	the levels	684:693	the levels of total glycosaminoglycans, proteins, and galactose	684:746	In contrast to INH, both preparations did not decrease the levels of total glycosaminoglycans, proteins, and galactose.					
31781998	0	29	theme	Extracellular	116:128	arg1	Matrix					130:135	Pulmonary Extracellular Matrix	106:135	Pulmonary Extracellular Matrix	106:135	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	1	30	with	mice	354:357	arg1	granulomatosis					376:389	BCG-induced granulomatosis	364:389	BCG-induced granulomatosis started from postinfection day 90	364:423	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	1	31	theme	hydrazide	226:234	arg1	injections					194:203	Intraperitoneal injections	178:203	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90	178:423	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	0	32	theme	Oxidized	32:39	arg1	Dextran					41:47	Oxidized Dextran	32:47	Oxidized Dextran	32:47	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	2	33	theme	liposomal	544:552	arg1	form					554:557	its liposomal form	540:557	its liposomal form	540:557	Both dextrazide and its liposomal form decreased the levels of sulfated glycosaminoglycans and uronic acids.					
31781998	4	34	theme	maintenance	1080:1090	arg1	down-regulation					1036:1050	down-regulation	1036:1050	down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group	1036:1153	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	2	35	theme	uronic	615:620	arg1	acids					622:626	uronic acids	615:626	uronic acids	615:626	Both dextrazide and its liposomal form decreased the levels of sulfated glycosaminoglycans and uronic acids.					
31781998	1	36	theme	BCG-induced	364:374	arg1	granulomatosis					376:389	BCG-induced granulomatosis	364:389	BCG-induced granulomatosis started from postinfection day 90	364:423	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	4	37	theme	liposomal	804:812	arg1	dextrazide					814:823	free and liposomal dextrazide	795:823	free and liposomal dextrazide	795:823	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	0	38	with	Delivery	20:27	arg1	Hydrazide					72:80	Isonicotinic Acid Hydrazide	54:80	Isonicotinic Acid Hydrazide	54:80	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	4	39	from	decrease	972:979	arg1	level					988:992	the level	984:992	the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group	984:1153	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	4	39	from	decrease	972:979	arg1	content					958:964	TIMP-2 content	951:964	TIMP-2 content	951:964	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	0	40	theme	BCG-Induced	150:160	arg1	Granulomatosis					162:175	BCG-Induced Granulomatosis	150:175	BCG-Induced Granulomatosis	150:175	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	1	41	theme	pulmonary	488:496	arg1	matrix					512:517	pulmonary extracellular matrix	488:517	pulmonary extracellular matrix	488:517	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	0	42	theme	Dextran	41:47	arg1	Delivery					20:27	Liposomal Delivery	10:27	Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide	10:80	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	1	43	theme	INH	315:317	arg1	dose					319:322	INH dose	315:322	INH dose of 14 mg/kg	315:334	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	4	44	theme	TIMP-1	876:881	arg1	content					865:871	the content	861:871	the content of TIMP-1 and TIMP-2	861:892	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	1	45	theme	extracellular	498:510	arg1	matrix					512:517	pulmonary extracellular matrix	488:517	pulmonary extracellular matrix	488:517	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	0	46	with	Mice	140:143	arg1	Granulomatosis					162:175	BCG-Induced Granulomatosis	150:175	BCG-Induced Granulomatosis	150:175	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	4	47	from	fibrosis	1124:1131	arg1	mice					1136:1139	mice	1136:1139	mice of this group	1136:1153	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	1	48	theme	matrix	512:517	arg1	composition					473:483	composition	473:483	composition of pulmonary extracellular matrix	473:517	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	4	49	theme	TIMP-2	887:892	arg1	content					865:871	the content	861:871	the content of TIMP-1 and TIMP-2	861:892	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	0	50	from	Profile	95:101	arg1	Mice					140:143	Mice	140:143	Mice with BCG-Induced Granulomatosis	140:175	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	4	51	theme	group	1149:1153	arg1	mice					1136:1139	mice	1136:1139	mice of this group	1136:1153	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	4	52	theme	collagen	1055:1062	arg1	degradation					1064:1074	collagen degradation	1055:1074	collagen degradation	1055:1074	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	0	53	theme	Acid	67:70	arg1	Hydrazide					72:80	Isonicotinic Acid Hydrazide	54:80	Isonicotinic Acid Hydrazide	54:80	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	1	54	theme	dextrazide	243:252	arg1	injections					194:203	Intraperitoneal injections	178:203	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90	178:423	Intraperitoneal injections of isonicotinic acid hydrazide (INH), dextrazide (oxidized dextran+INH), or liposomes loaded with dextrazide (INH dose of 14 mg/kg) over 2 months to mice with BCG-induced granulomatosis started from postinfection day 90 induced qualitative and quantitative changes in composition of pulmonary extracellular matrix.					
31781998	0	55	theme	Isonicotinic	54:65	arg1	Hydrazide					72:80	Isonicotinic Acid Hydrazide	54:80	Isonicotinic Acid Hydrazide	54:80	Effect of Liposomal Delivery of Oxidized Dextran with Isonicotinic Acid Hydrazide on Component Profile of Pulmonary Extracellular Matrix in Mice with BCG-Induced Granulomatosis.					
31781998	3	56	theme	total	698:702	arg1	glycosaminoglycans					704:721	total glycosaminoglycans	698:721	total glycosaminoglycans	698:721	In contrast to INH, both preparations did not decrease the levels of total glycosaminoglycans, proteins, and galactose.					
31781998	4	57	theme	free	795:798	arg1	dextrazide					814:823	free and liposomal dextrazide	795:823	free and liposomal dextrazide	795:823	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	4	58	theme	free	997:1000	arg1	hydroxyproline					1002:1015	free hydroxyproline	997:1015	free hydroxyproline	997:1015	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	4	59	from	increase	939:946	arg1	level					988:992	the level	984:992	the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group	984:1153	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
31781998	4	59	from	increase	939:946	arg1	content					958:964	TIMP-2 content	951:964	TIMP-2 content	951:964	This difference is explained by the fact both free and liposomal dextrazide activated MMP, but did not increase the content of TIMP-1 and TIMP-2, whereas injection of INH was followed by an increase in TIMP-2 content and a decrease in the level of free hydroxyproline, which attested to down-regulation of collagen degradation and maintenance of the conditions for pulmonary fibrosis in mice of this group.					
30900971	1	0	theme	99.5 	171:175	arg1	%					176:176	%	176:176	%	176:176	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	1	theme	same	407:410	arg1	position					422:429	the same taxonomic position	403:429	the same taxonomic position	403:429	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	3	2	theme	later	500:504	arg1	synonym					518:524	a later heterotypic synonym	498:524	a later heterotypic synonym of L. metriopterae	498:543	L. terrae is a later heterotypic synonym of L. metriopterae.					
30900971	3	2	theme	later	500:504	arg1	terrae					488:493	L. terrae	485:493	L. terrae	485:493	L. terrae is a later heterotypic synonym of L. metriopterae.					
30900971	1	3	theme	99.6 	251:255	arg1	%					256:256	%	256:256	%	256:256	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	4	theme	taxonomic	412:420	arg1	position					422:429	the same taxonomic position	403:429	the same taxonomic position	403:429	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	3	5	theme	heterotypic	506:516	arg1	synonym					518:524	a later heterotypic synonym	498:524	a later heterotypic synonym of L. metriopterae	498:543	L. terrae is a later heterotypic synonym of L. metriopterae.					
30900971	3	5	theme	heterotypic	506:516	arg1	terrae					488:493	L. terrae	485:493	L. terrae	485:493	L. terrae is a later heterotypic synonym of L. metriopterae.					
30900971	1	6	theme	%	256:256	arg1	similarity					277:286	99.6 % rpoA gene sequence similarity	251:286	99.6 % rpoA gene sequence similarity	251:286	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	7	theme	%	176:176	arg1	similarity					201:210	99.5 % 16S rRNA gene sequence similarity	171:210	99.5 % 16S rRNA gene sequence similarity	171:210	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	8	theme	16S	178:180	arg1	similarity					201:210	99.5 % 16S rRNA gene sequence similarity	171:210	99.5 % 16S rRNA gene sequence similarity	171:210	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	9	theme	silico	344:349	arg1	hybridization					359:371	silico DNA-DNA hybridization	344:371	silico DNA-DNA hybridization value	344:377	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	2	10	theme	L.	468:469	arg1	terrae					435:440	L. terrae	432:440	L. terrae	432:440	L. terrae should be reclassified as L. metriopterae.					
30900971	2	10	theme	L.	468:469	arg1	metriopterae					471:482	L. metriopterae	468:482	L. metriopterae	468:482	L. terrae should be reclassified as L. metriopterae.					
30900971	3	11	theme	L.	529:530	arg1	metriopterae					532:543	L. metriopterae	529:543	L. metriopterae	529:543	L. terrae is a later heterotypic synonym of L. metriopterae.					
30900971	1	12	theme	rRNA	182:185	arg1	similarity					201:210	99.5 % 16S rRNA gene sequence similarity	171:210	99.5 % 16S rRNA gene sequence similarity	171:210	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	0	13	theme	Lactobacillus	0:12	arg1	synonym					44:50	a later heterotypic synonym	24:50	a later heterotypic synonym of Lactobacillus metriopterae	24:80	Lactobacillus terrae is a later heterotypic synonym of Lactobacillus metriopterae.					
30900971	0	13	theme	Lactobacillus	0:12	arg1	terrae					14:19	Lactobacillus terrae	0:19	Lactobacillus terrae	0:19	Lactobacillus terrae is a later heterotypic synonym of Lactobacillus metriopterae.					
30900971	1	14	theme	rpoA	258:261	arg1	similarity					277:286	99.6 % rpoA gene sequence similarity	251:286	99.6 % rpoA gene sequence similarity	251:286	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	3	15	theme	metriopterae	532:543	arg1	synonym					518:524	a later heterotypic synonym	498:524	a later heterotypic synonym of L. metriopterae	498:543	L. terrae is a later heterotypic synonym of L. metriopterae.					
30900971	3	15	theme	metriopterae	532:543	arg1	terrae					488:493	L. terrae	485:493	L. terrae	485:493	L. terrae is a later heterotypic synonym of L. metriopterae.					
30900971	1	16	theme	gene	187:190	arg1	similarity					201:210	99.5 % 16S rRNA gene sequence similarity	171:210	99.5 % 16S rRNA gene sequence similarity	171:210	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	17	theme	gene	263:266	arg1	similarity					277:286	99.6 % rpoA gene sequence similarity	251:286	99.6 % rpoA gene sequence similarity	251:286	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	18	theme	sequence	192:199	arg1	similarity					201:210	99.5 % 16S rRNA gene sequence similarity	171:210	99.5 % 16S rRNA gene sequence similarity	171:210	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	19	from	%	339:339	arg1	value					373:377	silico DNA-DNA hybridization value	344:377	silico DNA-DNA hybridization value	344:377	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	20	theme	sequence	268:275	arg1	similarity					277:286	99.6 % rpoA gene sequence similarity	251:286	99.6 % rpoA gene sequence similarity	251:286	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	21	theme	DNA-DNA	351:357	arg1	hybridization					359:371	silico DNA-DNA hybridization	344:371	silico DNA-DNA hybridization value	344:377	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	0	22	theme	heterotypic	32:42	arg1	synonym					44:50	a later heterotypic synonym	24:50	a later heterotypic synonym of Lactobacillus metriopterae	24:80	Lactobacillus terrae is a later heterotypic synonym of Lactobacillus metriopterae.					
30900971	0	22	theme	heterotypic	32:42	arg1	terrae					14:19	Lactobacillus terrae	0:19	Lactobacillus terrae	0:19	Lactobacillus terrae is a later heterotypic synonym of Lactobacillus metriopterae.					
30900971	1	23	theme	hybridization	359:371	arg1	value					373:377	silico DNA-DNA hybridization value	344:377	silico DNA-DNA hybridization value	344:377	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	0	24	theme	later	26:30	arg1	synonym					44:50	a later heterotypic synonym	24:50	a later heterotypic synonym of Lactobacillus metriopterae	24:80	Lactobacillus terrae is a later heterotypic synonym of Lactobacillus metriopterae.					
30900971	0	24	theme	later	26:30	arg1	terrae					14:19	Lactobacillus terrae	0:19	Lactobacillus terrae	0:19	Lactobacillus terrae is a later heterotypic synonym of Lactobacillus metriopterae.					
30900971	1	25	dep	%	218:218	arg1	pheS					220:223	pheS	220:223	pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value	220:328	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	26	theme	%	294:294	arg1	identity					315:322	% average nucleotide identity	294:322	% average nucleotide identity	294:322	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	27	theme	average	296:302	arg1	identity					315:322	% average nucleotide identity	294:322	% average nucleotide identity	294:322	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	28	from	%	218:218	arg1	value					373:377	silico DNA-DNA hybridization value	344:377	silico DNA-DNA hybridization value	344:377	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	29	dep	similarity	239:248	arg1	value					324:328	value	324:328	value	324:328	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	0	30	theme	metriopterae	69:80	arg1	synonym					44:50	a later heterotypic synonym	24:50	a later heterotypic synonym of Lactobacillus metriopterae	24:80	Lactobacillus terrae is a later heterotypic synonym of Lactobacillus metriopterae.					
30900971	0	30	theme	metriopterae	69:80	arg1	terrae					14:19	Lactobacillus terrae	0:19	Lactobacillus terrae	0:19	Lactobacillus terrae is a later heterotypic synonym of Lactobacillus metriopterae.					
30900971	1	31	contain	have	398:401	arg2	position					422:429	the same taxonomic position	403:429	the same taxonomic position	403:429	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	31	contain	have	398:401	arg1	they					393:396	they	393:396	they	393:396	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	32	theme	gene	225:228	arg1	similarity					239:248	gene sequence similarity	225:248	gene sequence similarity	225:248	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	0	33	theme	Lactobacillus	55:67	arg1	metriopterae					69:80	Lactobacillus metriopterae	55:80	Lactobacillus metriopterae	55:80	Lactobacillus terrae is a later heterotypic synonym of Lactobacillus metriopterae.					
30900971	1	34	theme	nucleotide	304:313	arg1	identity					315:322	% average nucleotide identity	294:322	% average nucleotide identity	294:322	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
30900971	1	35	theme	sequence	230:237	arg1	similarity					239:248	gene sequence similarity	225:248	gene sequence similarity	225:248	Lactobacillus metriopterae and Lactobacillus terrae are closely related, and they share 99.5 % 16S rRNA gene sequence similarity, 98.4 % pheS gene sequence similarity, 99.6 % rpoA gene sequence similarity, 97.9 % average nucleotide identity value and 81.3 % in silico DNA-DNA hybridization value, showing that they have the same taxonomic position.					
31751685	4	0	theme	mechanical	909:918	arg1	properties					932:941	mechanical compression properties	909:941	mechanical compression properties	909:941	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	10	1	theme	polymeric	1880:1888	arg1	scaffolds					1900:1908	biodegradable polymeric composite scaffolds	1866:1908	biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering	1866:1987	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	9	2	theme	bimodal	1656:1662	arg1	scaffolds					1676:1684	the obtained bimodal open-porous scaffolds	1643:1684	the obtained bimodal open-porous scaffolds	1643:1684	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	7	3	theme	contact	1430:1436	arg1	angle					1438:1442	water contact angle	1424:1442	water contact angle of 71.7 °C	1424:1453	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	3	4	from	depressurization	672:687	arg1	process					740:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	3	5	theme	open-porous	562:572	arg1	architecture					583:594	The bimodal open-porous scaffold architecture	550:594	The bimodal open-porous scaffold architecture	550:594	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	4	6	theme	degradation	880:890	arg1	performance					892:902	degradation performance	880:902	degradation performance	880:902	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	10	7	with	scaffolds	1900:1908	arg1	properties					1956:1965	balanced properties	1947:1965	balanced properties	1947:1965	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	10	7	with	scaffolds	1900:1908	arg1	structure					1933:1941	bimodal open-pore structure	1915:1941	bimodal open-pore structure	1915:1941	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	10	8	theme	bimodal	1915:1921	arg1	structure					1933:1941	bimodal open-pore structure	1915:1941	bimodal open-pore structure	1915:1941	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	7	9	theme	71.7 °C	1447:1453	arg1	angle					1438:1442	water contact angle	1424:1442	water contact angle of 71.7 °C	1424:1453	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	1	10	theme	open-pore	205:213	arg1	architecture					215:226	multiscale open-pore architecture	194:226	multiscale open-pore architecture (i.e., bimodal structure)	194:252	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	6	11	dep	pore	1217:1220	arg1	~11.0 μm					1223:1230	~11.0 μm	1223:1230	~11.0 μm in diameter	1223:1242	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	5	12	with	scaffold	983:990	arg1	structure					1011:1019	unimodal pore structure	997:1019	unimodal pore structure	997:1019	In the experiments, the scaffold with unimodal pore structure was used for comparison.					
31751685	10	13	theme	balanced	1947:1954	arg1	properties					1956:1965	balanced properties	1947:1965	balanced properties	1947:1965	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	7	14	theme	50	1395:1396	arg1	%					1397:1397	%	1397:1397	%	1397:1397	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	10	15	from	structure	1933:1941	arg1	engineering					1977:1987	tissue engineering	1970:1987	tissue engineering	1970:1987	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	1	16	dep	structure	243:251	arg1	i.e.					229:232	i.e.	229:232	i.e.	229:232	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	7	17	theme	2.76 MPa	1383:1390	arg1	strength					1371:1378	compressive strength	1359:1378	compressive strength of 2.76 MPa at 50% strain	1359:1404	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	3	18	from	process	740:746	arg1	control					624:630	synergistic control	612:630	synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process	612:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	3	19	theme	synergistic	612:622	arg1	control					624:630	synergistic control	612:630	synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process	612:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	7	20	from	strain	1399:1404	arg1	strength					1371:1378	compressive strength	1359:1378	compressive strength of 2.76 MPa at 50% strain	1359:1404	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	9	21	dep	98	1778:1779	arg1	to					1775:1776	to	1775:1776	to	1775:1776	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	9	22	theme	7th	1788:1790	arg1	day					1792:1794	7th day	1788:1794	7th day of culture	1788:1805	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	2	23	theme	interconnected	496:509	arg1	structure					511:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	2	24	theme	nanocrystals	406:417	arg1	scaffold					436:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold	355:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure	355:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	3	25	theme	variation	647:655	arg1	control					624:630	synergistic control	612:630	synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process	612:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	1	26	theme	porous	154:159	arg1	engineering					168:178	porous tissue engineering	154:178	porous tissue engineering scaffold	154:187	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	2	27	theme	bimodal	478:484	arg1	structure					511:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	1	28	theme	engineering	168:178	arg1	scaffold					180:187	porous tissue engineering scaffold	154:187	porous tissue engineering scaffold	154:187	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	4	29	theme	PBS/CNCs	810:817	arg1	scaffold					819:826	the bimodal open-porous PBS/CNCs scaffold	786:826	the bimodal open-porous PBS/CNCs scaffold	786:826	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	10	30	from	properties	1956:1965	arg1	engineering					1977:1987	tissue engineering	1970:1987	tissue engineering	1970:1987	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	3	31	from	variation	647:655	arg1	process					740:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	0	32	theme	tissue	108:113	arg1	engineering					115:125	tissue engineering	108:125	tissue engineering application	108:137	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose nanocrystals composite scaffolds for tissue engineering application.					
31751685	1	33	with	design	144:149	arg1	architecture					215:226	multiscale open-pore architecture	194:226	multiscale open-pore architecture (i.e., bimodal structure)	194:252	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	6	34	theme	well-defined	1120:1131	arg1	structure					1151:1159	a well-defined bimodal open-pore structure	1118:1159	a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter)	1118:1243	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	2	35	theme	composite	426:434	arg1	scaffold					436:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold	355:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure	355:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	7	36	dep	in	1460:1461	arg1	vitro					1463:1467	vitro	1463:1467	vitro	1463:1467	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	7	37	theme	mechanical	1324:1333	arg1	rate					1481:1484	in vitro degradation rate	1460:1484	in vitro degradation rate	1460:1484	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	7	37	theme	mechanical	1324:1333	arg1	hydrophilicity					1408:1421	hydrophilicity	1408:1421	hydrophilicity (water contact angle of 71.7 °C)	1408:1454	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	7	37	theme	mechanical	1324:1333	arg1	properties					1347:1356	good mechanical compressive properties	1319:1356	good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain)	1319:1405	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	2	38	theme	CNCs	420:423	arg1	scaffold					436:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold	355:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure	355:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	4	39	theme	bimodal	790:796	arg1	scaffold					819:826	the bimodal open-porous PBS/CNCs scaffold	786:826	the bimodal open-porous PBS/CNCs scaffold	786:826	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	5	40	used	used	1025:1028	arg2	scaffold					983:990	the scaffold	979:990	the scaffold with unimodal pore structure	979:1019	In the experiments, the scaffold with unimodal pore structure was used for comparison.					
31751685	0	41	theme	bimodal	15:21	arg1	/cellulose					60:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose nanocrystals composite scaffolds for tissue engineering application.					
31751685	5	42	theme	unimodal	997:1004	arg1	structure					1011:1019	unimodal pore structure	997:1019	unimodal pore structure	997:1019	In the experiments, the scaffold with unimodal pore structure was used for comparison.					
31751685	6	43	theme	PBS5	1094:1097	arg1	scaffold					1099:1106	the bimodal open-porous PBS5 scaffold	1070:1106	the bimodal open-porous PBS5 scaffold	1070:1106	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	2	44	theme	well-defined	452:463	arg1	structure					511:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	8	45	theme	fibroblast	1551:1560	arg1	cells					1562:1566	NIH-3T3 fibroblast cells	1543:1566	NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay	1543:1617	Moreover, in vitro biocompatibility was determined with NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay.					
31751685	10	46	theme	new	1837:1839	arg1	insights					1841:1848	new insights	1837:1848	new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering	1837:1987	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	9	47	contain	had	1686:1688	arg2	biocompatibility					1697:1712	a good biocompatibility	1690:1712	a good biocompatibility	1690:1712	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	9	47	contain	had	1686:1688	arg1	scaffolds					1676:1684	the obtained bimodal open-porous scaffolds	1643:1684	the obtained bimodal open-porous scaffolds	1643:1684	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	0	48	theme	poly	35:38	arg1	/cellulose					60:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose nanocrystals composite scaffolds for tissue engineering application.					
31751685	6	49	theme	bimodal	1074:1080	arg1	scaffold					1099:1106	the bimodal open-porous PBS5 scaffold	1070:1106	the bimodal open-porous PBS5 scaffold	1070:1106	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	3	50	theme	depressurization	672:687	arg1	control					624:630	synergistic control	612:630	synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process	612:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	4	51	theme	hydrophilic	864:874	arg1	porosity					854:861	open porosity	849:861	open porosity	849:861	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	0	52	theme	butylene	41:48	arg1	/cellulose					60:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose nanocrystals composite scaffolds for tissue engineering application.					
31751685	7	53	dep	properties	1347:1356	arg1	strength					1371:1378	compressive strength	1359:1378	compressive strength of 2.76 MPa at 50% strain	1359:1404	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	9	54	theme	open-porous	1664:1674	arg1	scaffolds					1676:1684	the obtained bimodal open-porous scaffolds	1643:1684	the obtained bimodal open-porous scaffolds	1643:1684	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	6	55	dep	pore	1179:1182	arg1	~68.9 μm					1185:1192	~68.9 μm	1185:1192	~68.9 μm in diameter	1185:1204	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	1	56	theme	bimodal	235:241	arg1	structure					243:251	bimodal structure	235:251	bimodal structure	235:251	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	6	57	theme	small	1211:1215	arg1	pore					1217:1220	small pore	1211:1220	small pore (~11.0 μm in diameter)	1211:1243	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	6	58	theme	large	1173:1177	arg1	pore					1179:1182	large pore	1173:1182	large pore (~68.9 μm in diameter)	1173:1205	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	8	59	theme	live/dead	1588:1596	arg1	assay					1613:1617	live/dead cell viability assay	1588:1617	live/dead cell viability assay	1588:1617	Moreover, in vitro biocompatibility was determined with NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay.					
31751685	3	60	theme	carbon	708:713	arg1	Sc-CO2					724:729	Sc-CO2	724:729	Sc-CO2	724:729	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	3	60	theme	carbon	708:713	arg1	dioxide					715:721	supercritical carbon dioxide	694:721	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	7	61	theme	degradation	1469:1479	arg1	properties					1347:1356	good mechanical compressive properties	1319:1356	good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain)	1319:1405	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	7	61	theme	degradation	1469:1479	arg1	rate					1481:1484	in vitro degradation rate	1460:1484	in vitro degradation rate	1460:1484	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	8	62	theme	viability	1603:1611	arg1	assay					1613:1617	live/dead cell viability assay	1588:1617	live/dead cell viability assay	1588:1617	Moreover, in vitro biocompatibility was determined with NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay.					
31751685	10	63	theme	composite	1890:1898	arg1	scaffolds					1900:1908	biodegradable polymeric composite scaffolds	1866:1908	biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering	1866:1987	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	2	64	theme	porous	357:362	arg1	poly					364:367	porous poly	357:367	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure	355:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	2	64	theme	porous	357:362	arg1	succinate					379:387	butylene succinate	370:387	butylene succinate	370:387	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	2	64	theme	porous	357:362	arg1	PBS					391:393	PBS	391:393	PBS	391:393	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	8	65	dep	in	1497:1498	arg1	vitro					1500:1504	vitro	1500:1504	vitro	1500:1504	Moreover, in vitro biocompatibility was determined with NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay.					
31751685	7	66	theme	in	1460:1461	arg1	properties					1347:1356	good mechanical compressive properties	1319:1356	good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain)	1319:1405	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	7	66	theme	in	1460:1461	arg1	rate					1481:1484	in vitro degradation rate	1460:1484	in vitro degradation rate	1460:1484	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	9	67	theme	obtained	1647:1654	arg1	scaffolds					1676:1684	the obtained bimodal open-porous scaffolds	1643:1684	the obtained bimodal open-porous scaffolds	1643:1684	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	6	68	theme	open-pore	1141:1149	arg1	structure					1151:1159	a well-defined bimodal open-pore structure	1118:1159	a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter)	1118:1243	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	10	69	theme	biodegradable	1866:1878	arg1	scaffolds					1900:1908	biodegradable polymeric composite scaffolds	1866:1908	biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering	1866:1987	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	3	70	theme	bimodal	554:560	arg1	architecture					583:594	The bimodal open-porous scaffold architecture	550:594	The bimodal open-porous scaffold architecture	550:594	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	4	71	theme	compression	920:930	arg1	properties					932:941	mechanical compression properties	909:941	mechanical compression properties	909:941	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	10	72	theme	open-pore	1923:1931	arg1	structure					1933:1941	bimodal open-pore structure	1915:1941	bimodal open-pore structure	1915:1941	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	1	73	theme	scaffold	180:187	arg1	design					144:149	The design	140:149	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure)	140:252	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	7	74	theme	water	1424:1428	arg1	angle					1438:1442	water contact angle	1424:1442	water contact angle of 71.7 °C	1424:1453	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	1	75	theme	multiscale	194:203	arg1	architecture					215:226	multiscale open-pore architecture	194:226	multiscale open-pore architecture (i.e., bimodal structure)	194:252	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	4	76	theme	performance	892:902	arg1	porosity					854:861	open porosity	849:861	open porosity	849:861	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	3	77	theme	scaffold	574:581	arg1	architecture					583:594	The bimodal open-porous scaffold architecture	550:594	The bimodal open-porous scaffold architecture	550:594	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	7	78	dep	hydrophilicity	1408:1421	arg1	angle					1438:1442	water contact angle	1424:1442	water contact angle of 71.7 °C	1424:1453	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	9	79	theme	good	1692:1695	arg1	biocompatibility					1697:1712	a good biocompatibility	1690:1712	a good biocompatibility	1690:1712	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	6	80	theme	high	1253:1256	arg1	porosity					1263:1270	a high open porosity	1251:1270	a high open porosity (~95.2%)	1251:1279	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	6	80	theme	high	1253:1256	arg1	%					1278:1278	~95.2%	1273:1278	~95.2%	1273:1278	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	9	81	theme	cells	1735:1739	arg1	viability					1722:1730	the viability	1718:1730	the viability of cells grown on the scaffolds	1718:1762	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	10	82	theme	tissue	1970:1975	arg1	engineering					1977:1987	tissue engineering	1970:1987	tissue engineering	1970:1987	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	1	83	dep	cell	263:266	arg1	attachment					268:277	attachment	268:277	attachment	268:277	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	0	84	theme	/cellulose	60:69	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose	0:69	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose nanocrystals composite scaffolds for tissue engineering application.					
31751685	3	85	theme	temperature	635:645	arg1	variation					647:655	temperature variation	635:655	temperature variation	635:655	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	2	86	with	scaffold	436:443	arg1	structure					511:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	2	87	theme	open-pore	486:494	arg1	structure					511:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	7	88	theme	compressive	1359:1369	arg1	strength					1371:1378	compressive strength	1359:1378	compressive strength of 2.76 MPa at 50% strain	1359:1404	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	0	89	theme	composite	84:92	arg1	scaffolds					94:102	composite scaffolds	84:102	composite scaffolds	84:102	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose nanocrystals composite scaffolds for tissue engineering application.					
31751685	1	90	theme	tissue	161:166	arg1	engineering					168:178	porous tissue engineering	154:178	porous tissue engineering scaffold	154:187	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	2	91	theme	controllable	465:476	arg1	structure					511:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	a well-defined controllable bimodal open-pore interconnected structure	450:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	8	92	theme	in	1497:1498	arg1	biocompatibility					1506:1521	in vitro biocompatibility	1497:1521	in vitro biocompatibility	1497:1521	Moreover, in vitro biocompatibility was determined with NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay.					
31751685	5	93	theme	pore	1006:1009	arg1	structure					1011:1019	unimodal pore structure	997:1019	unimodal pore structure	997:1019	In the experiments, the scaffold with unimodal pore structure was used for comparison.					
31751685	4	94	theme	open-porous	798:808	arg1	scaffold					819:826	the bimodal open-porous PBS/CNCs scaffold	786:826	the bimodal open-porous PBS/CNCs scaffold	786:826	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	0	95	theme	engineering	115:125	arg1	application					127:137	tissue engineering application	108:137	tissue engineering application	108:137	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose nanocrystals composite scaffolds for tissue engineering application.					
31751685	7	96	theme	compressive	1335:1345	arg1	rate					1481:1484	in vitro degradation rate	1460:1484	in vitro degradation rate	1460:1484	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	7	96	theme	compressive	1335:1345	arg1	hydrophilicity					1408:1421	hydrophilicity	1408:1421	hydrophilicity (water contact angle of 71.7 °C)	1408:1454	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	7	96	theme	compressive	1335:1345	arg1	properties					1347:1356	good mechanical compressive properties	1319:1356	good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain)	1319:1405	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	4	97	theme	scaffold	819:826	arg1	morphology					837:846	morphology	837:846	morphology	837:846	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	4	97	theme	scaffold	819:826	arg1	porosity					854:861	open porosity	849:861	open porosity	849:861	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	4	97	theme	scaffold	819:826	arg1	properties					772:781	The microstructure and properties	749:781	properties	772:781	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	4	97	theme	scaffold	819:826	arg1	microstructure					753:766	The microstructure and properties	749:781	microstructure	753:766	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	4	97	theme	scaffold	819:826	arg1	properties					932:941	mechanical compression properties	909:941	mechanical compression properties	909:941	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	6	98	from	~11.0 μm	1223:1230	arg1	diameter					1235:1242	diameter	1235:1242	diameter	1235:1242	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	7	99	theme	good	1319:1322	arg1	rate					1481:1484	in vitro degradation rate	1460:1484	in vitro degradation rate	1460:1484	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	7	99	theme	good	1319:1322	arg1	hydrophilicity					1408:1421	hydrophilicity	1408:1421	hydrophilicity (water contact angle of 71.7 °C)	1408:1454	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	7	99	theme	good	1319:1322	arg1	properties					1347:1356	good mechanical compressive properties	1319:1356	good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain)	1319:1405	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	8	100	theme	NIH-3T3	1543:1549	arg1	cells					1562:1566	NIH-3T3 fibroblast cells	1543:1566	NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay	1543:1617	Moreover, in vitro biocompatibility was determined with NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay.					
31751685	2	101	theme	butylene	370:377	arg1	poly					364:367	porous poly	357:367	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure	355:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	2	101	theme	butylene	370:377	arg1	succinate					379:387	butylene succinate	370:387	butylene succinate	370:387	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	0	102	theme	open-porous	23:33	arg1	/cellulose					60:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose nanocrystals composite scaffolds for tissue engineering application.					
31751685	6	103	theme	open	1258:1261	arg1	porosity					1263:1270	a high open porosity	1251:1270	a high open porosity (~95.2%)	1251:1279	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	6	103	theme	open	1258:1261	arg1	%					1278:1278	~95.2%	1273:1278	~95.2%	1273:1278	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	9	104	theme	culture	1799:1805	arg1	day					1792:1794	7th day	1788:1794	7th day of culture	1788:1805	Results indicated that the obtained bimodal open-porous scaffolds had a good biocompatibility and the viability of cells grown on the scaffolds reached up to 98% after 7th day of culture.					
31751685	3	105	from	control	624:630	arg1	process					740:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	2	106	theme	/cellulose	395:404	arg1	scaffold					436:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold	355:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure	355:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
31751685	6	107	theme	open-porous	1082:1092	arg1	scaffold					1099:1106	the bimodal open-porous PBS5 scaffold	1070:1106	the bimodal open-porous PBS5 scaffold	1070:1106	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	6	108	theme	bimodal	1133:1139	arg1	structure					1151:1159	a well-defined bimodal open-pore structure	1118:1159	a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter)	1118:1243	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	8	109	theme	MTT	1574:1576	arg1	assay					1578:1582	MTT assay	1574:1582	MTT assay	1574:1582	Moreover, in vitro biocompatibility was determined with NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay.					
31751685	3	110	theme	two-step	663:670	arg1	depressurization					672:687	a two-step depressurization	661:687	a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process	661:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	4	111	theme	open	849:852	arg1	porosity					854:861	open porosity	849:861	open porosity	849:861	The microstructure and properties of the bimodal open-porous PBS/CNCs scaffold, such as morphology, open porosity, hydrophilic and degradation performance, and mechanical compression properties, were analyzed.					
31751685	0	112	theme	succinate	50:58	arg1	/cellulose					60:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	bimodal open-porous poly (butylene succinate)/cellulose	15:69	Fabrication of bimodal open-porous poly (butylene succinate)/cellulose nanocrystals composite scaffolds for tissue engineering application.					
31751685	1	113	theme	oxygen	322:327	arg1	diffusion					329:337	oxygen diffusion	322:337	oxygen diffusion	322:337	The design of porous tissue engineering scaffold with multiscale open-pore architecture (i.e., bimodal structure) promotes cell attachment and growth, which facilitates nutrient and oxygen diffusion.					
31751685	10	114	theme	scaffolds	1900:1908	arg1	use					1859:1861	the use	1855:1861	the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering	1855:1987	Therefore, our work provides new insights into the use of biodegradable polymeric composite scaffolds with bimodal open-pore structure and balanced properties in tissue engineering.					
31751685	3	115	theme	dioxide	715:721	arg1	process					740:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	6	116	from	~68.9 μm	1185:1192	arg1	diameter					1197:1204	diameter	1197:1204	diameter	1197:1204	The results showed that the bimodal open-porous PBS5 scaffold displayed a well-defined bimodal open-pore structure composed of large pore (~68.9 μm in diameter) and small pore (~11.0 μm in diameter), with a high open porosity (~95.2%).					
31751685	7	117	theme	%	1397:1397	arg1	strain					1399:1404	50% strain	1395:1404	50% strain	1395:1404	In addition, the scaffolds exhibited good mechanical compressive properties (compressive strength of 2.76 MPa at 50% strain), hydrophilicity (water contact angle of 71.7 °C) and in vitro degradation rate.					
31751685	8	118	theme	cell	1598:1601	arg1	assay					1613:1617	live/dead cell viability assay	1588:1617	live/dead cell viability assay	1588:1617	Moreover, in vitro biocompatibility was determined with NIH-3T3 fibroblast cells using MTT assay and live/dead cell viability assay.					
31751685	3	119	theme	supercritical	694:706	arg1	Sc-CO2					724:729	Sc-CO2	724:729	Sc-CO2	724:729	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	3	119	theme	supercritical	694:706	arg1	dioxide					715:721	supercritical carbon dioxide	694:721	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	3	120	theme	foaming	732:738	arg1	process					740:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	a supercritical carbon dioxide (Sc-CO2) foaming process	692:746	The bimodal open-porous scaffold architecture was designed by synergistic control of temperature variation and a two-step depressurization in a supercritical carbon dioxide (Sc-CO2) foaming process.					
31751685	2	121	theme	poly	364:367	arg1	scaffold					436:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold	355:443	a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure	355:519	In this study, a porous poly (butylene succinate) (PBS)/cellulose nanocrystals (CNCs) composite scaffold with a well-defined controllable bimodal open-pore interconnected structure was successfully fabricated.					
30950465	0	0	theme	bioactive	75:83	arg1	properties					85:94	bioactive properties	75:94	bioactive properties	75:94	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	1	1	theme	Syrah	139:143	arg1	extract					158:164	Syrah grape pomace extract	139:164	Syrah grape pomace extract.	139:165	Syrah grape pomace extract.					
30950465	11	2	theme	different	1879:1887	arg1	properties					1900:1909	different biological properties	1879:1909	different biological properties	1879:1909	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	5	3	theme	ingredient	880:889	arg1	safety					865:870	The safety	861:870	The safety of this ingredient after GID	861:899	The safety of this ingredient after GID was also assessed.					
30950465	11	4	theme	foods	2042:2046	arg1	development					2012:2022	the development	2008:2022	the development of new functional foods	2008:2046	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	2	5	theme	phenolic	286:293	arg1	compounds					295:303	phenolic compounds	286:303	phenolic compounds	286:303	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	6	6	theme	GP	920:921	arg1	extract					923:929	GP extract	920:929	GP extract	920:929	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	0	7	from	Impact	0:5	arg1	composition					62:72	the chemical composition	49:72	the chemical composition	49:72	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	0	7	from	Impact	0:5	arg1	properties					85:94	bioactive properties	75:94	bioactive properties	75:94	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	0	7	from	Impact	0:5	arg1	cytotoxicity					101:112	cytotoxicity	101:112	cytotoxicity	101:112	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	9	8	theme	antimicrobial	1570:1582	arg1	capacity					1584:1591	the antimicrobial capacity	1566:1591	the antimicrobial capacity	1566:1591	The extract also exhibited strong antioxidant and antimicrobial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa; however, after GID, the antioxidant capacity decreased, and the antimicrobial capacity was strongly reduced or lost.					
30950465	3	9	theme	chemical	441:448	arg1	composition					450:460	chemical composition	441:460	chemical composition	441:460	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	8	10	theme	potential	1242:1250	arg1	source					1259:1264	a potential carbon source	1240:1264	a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion	1240:1351	The use of 2% (w/v) of this ingredient proved to be a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion.					
30950465	8	10	theme	potential	1242:1250	arg1	use					1192:1194	The use	1188:1194	The use of 2% (w/v) of this ingredient	1188:1225	The use of 2% (w/v) of this ingredient proved to be a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion.					
30950465	3	11	theme	enzymatic	494:502	arg1	extract					507:513	an enzymatic GP extract	491:513	an enzymatic GP extract	491:513	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	7	12	theme	gastric	1141:1147	arg1	conditions					1149:1158	gastric conditions	1141:1158	gastric conditions	1141:1158	In vitro simulated GID revealed that xylobiose was resistant to gastric conditions, unlike phenolic compounds.					
30950465	3	13	dep	composition	450:460	arg1	the					437:439	the	437:439	the	437:439	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	10	14	theme	extract	1640:1646	arg1	safety					1648:1653	the extract safety	1636:1653	the extract safety	1636:1653	Furthermore, the extract safety was also guaranteed on Caco-2 intestinal cells.					
30950465	11	15	theme	bioactive	1769:1777	arg1	acids					1843:1847	organic acids	1835:1847	organic acids	1835:1847	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	15	theme	bioactive	1769:1777	arg1	minerals					1854:1861	minerals	1854:1861	minerals	1854:1861	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	15	theme	bioactive	1769:1777	arg1	polyphenols					1822:1832	polyphenols	1822:1832	polyphenols	1822:1832	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	15	theme	bioactive	1769:1777	arg1	molecules					1779:1787	relevant bioactive molecules	1760:1787	relevant bioactive molecules	1760:1787	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	15	theme	bioactive	1769:1777	arg1	xylooligosaccharides					1800:1819	xylooligosaccharides	1800:1819	xylooligosaccharides	1800:1819	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	16	theme	relevant	1760:1767	arg1	acids					1843:1847	organic acids	1835:1847	organic acids	1835:1847	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	16	theme	relevant	1760:1767	arg1	minerals					1854:1861	minerals	1854:1861	minerals	1854:1861	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	16	theme	relevant	1760:1767	arg1	polyphenols					1822:1832	polyphenols	1822:1832	polyphenols	1822:1832	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	16	theme	relevant	1760:1767	arg1	molecules					1779:1787	relevant bioactive molecules	1760:1787	relevant bioactive molecules	1760:1787	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	16	theme	relevant	1760:1767	arg1	xylooligosaccharides					1800:1819	xylooligosaccharides	1800:1819	xylooligosaccharides	1800:1819	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	4	17	theme	chemical	768:775	arg1	composition					777:787	its chemical composition	764:787	its chemical composition	764:787	GP extract was previously produced using an enzymatic cocktail with xylanase activity and was then exposed to simulated conditions of GID, characterized for its chemical composition, and screened for antimicrobial, prebiotic, and antioxidant activities.					
30950465	1	18	theme	pomace	151:156	arg1	extract					158:164	Syrah grape pomace extract	139:164	Syrah grape pomace extract.	139:165	Syrah grape pomace extract.					
30950465	7	19	dep	In	1077:1078	arg1	vitro					1080:1084	vitro	1080:1084	vitro	1080:1084	In vitro simulated GID revealed that xylobiose was resistant to gastric conditions, unlike phenolic compounds.					
30950465	3	20	theme	biological	466:475	arg1	activities					477:486	biological activities	466:486	biological activities	466:486	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	11	21	theme	organic	1835:1841	arg1	acids					1843:1847	organic acids	1835:1847	organic acids	1835:1847	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	10	22	theme	Caco-2	1678:1683	arg1	cells					1696:1700	Caco-2 intestinal cells	1678:1700	Caco-2 intestinal cells	1678:1700	Furthermore, the extract safety was also guaranteed on Caco-2 intestinal cells.					
30950465	11	23	theme	new	2027:2029	arg1	foods					2042:2046	new functional foods	2027:2046	new functional foods	2027:2046	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	3	24	theme	GP	504:505	arg1	extract					507:513	an enzymatic GP extract	491:513	an enzymatic GP extract	491:513	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	2	25	theme	gastrointestinal	369:384	arg1	health					386:391	gastrointestinal health	369:391	gastrointestinal health	369:391	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	11	26	theme	green	1718:1722	arg1	extract					1727:1733	This novel and green GP extract	1703:1733	This novel and green GP extract	1703:1733	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	27	theme	functional	2031:2040	arg1	foods					2042:2046	new functional foods	2027:2046	new functional foods	2027:2046	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	3	28	theme	extract	507:513	arg1	activities					477:486	biological activities	466:486	biological activities	466:486	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	3	28	theme	extract	507:513	arg1	composition					450:460	chemical composition	441:460	chemical composition	441:460	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	11	29	theme	GP	1724:1725	arg1	extract					1727:1733	This novel and green GP extract	1703:1733	This novel and green GP extract	1703:1733	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	0	30	theme	Vitis	117:121	arg1	vinifera					123:130	Vitis vinifera	117:130	Vitis vinifera	117:130	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	2	31	theme	bioactive	246:254	arg1	fibers					275:280	fibers	275:280	fibers	275:280	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	2	31	theme	bioactive	246:254	arg1	compounds					295:303	phenolic compounds	286:303	phenolic compounds	286:303	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	2	31	theme	bioactive	246:254	arg1	compounds					256:264	its bioactive compounds	242:264	its bioactive compounds	242:264	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	11	32	used	used	1983:1986	arg2	ingredient					1994:2003	an ingredient	1991:2003	an ingredient in the development of new functional foods	1991:2046	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	32	used	used	1983:1986	arg2	it					1973:1974	it	1973:1974	it	1973:1974	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	6	33	theme	other	976:980	arg1	carbohydrates					982:994	other carbohydrates	976:994	other carbohydrates	976:994	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	6	33	theme	other	976:980	arg1	xylooligosaccharides					1007:1026	xylooligosaccharides	1007:1026	xylooligosaccharides	1007:1026	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	0	34	theme	in	10:11	arg1	digestion					36:44	in vitro gastrointestinal digestion	10:44	in vitro gastrointestinal digestion	10:44	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	11	35	theme	novel	1708:1712	arg1	extract					1727:1733	This novel and green GP extract	1703:1733	This novel and green GP extract	1703:1733	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	4	36	theme	antioxidant	837:847	arg1	activities					849:858	antioxidant activities	837:858	antioxidant activities	837:858	GP extract was previously produced using an enzymatic cocktail with xylanase activity and was then exposed to simulated conditions of GID, characterized for its chemical composition, and screened for antimicrobial, prebiotic, and antioxidant activities.					
30950465	2	37	theme	Grape	167:171	arg1	GP					181:182	GP	181:182	GP	181:182	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	2	37	theme	Grape	167:171	arg1	pomace					173:178	Grape pomace	167:178	Grape pomace (GP)	167:183	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	1	38	theme	grape	145:149	arg1	extract					158:164	Syrah grape pomace extract	139:164	Syrah grape pomace extract.	139:165	Syrah grape pomace extract.					
30950465	2	39	theme	human	345:349	arg1	health					351:356	human health	345:356	human health	345:356	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	0	40	theme	digestion	36:44	arg1	Impact					0:5	Impact	0:5	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera	0:130	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	9	41	theme	antimicrobial	1404:1416	arg1	activities					1418:1427	strong antioxidant and antimicrobial activities	1381:1427	strong antioxidant and antimicrobial activities	1381:1427	The extract also exhibited strong antioxidant and antimicrobial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa; however, after GID, the antioxidant capacity decreased, and the antimicrobial capacity was strongly reduced or lost.					
30950465	9	42	theme	strong	1381:1386	arg1	activities					1418:1427	strong antioxidant and antimicrobial activities	1381:1427	strong antioxidant and antimicrobial activities	1381:1427	The extract also exhibited strong antioxidant and antimicrobial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa; however, after GID, the antioxidant capacity decreased, and the antimicrobial capacity was strongly reduced or lost.					
30950465	2	43	theme	byproduct	196:204	arg1	worldwide					206:214	a major byproduct worldwide	188:214	a major byproduct worldwide	188:214	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	6	44	theme	fiber	966:970	arg1	contents					946:953	high contents	941:953	high contents of dietary fiber	941:970	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	6	44	theme	fiber	966:970	arg1	carbohydrates					982:994	other carbohydrates	976:994	other carbohydrates	976:994	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	6	44	theme	fiber	966:970	arg1	xylooligosaccharides					1007:1026	xylooligosaccharides	1007:1026	xylooligosaccharides	1007:1026	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	7	45	theme	simulated	1086:1094	arg1	GID					1096:1098	In vitro simulated GID	1077:1098	In vitro simulated GID	1077:1098	In vitro simulated GID revealed that xylobiose was resistant to gastric conditions, unlike phenolic compounds.					
30950465	0	46	theme	vinifera	123:130	arg1	composition					62:72	the chemical composition	49:72	the chemical composition	49:72	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	0	46	theme	vinifera	123:130	arg1	properties					85:94	bioactive properties	75:94	bioactive properties	75:94	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	0	46	theme	vinifera	123:130	arg1	cytotoxicity					101:112	cytotoxicity	101:112	cytotoxicity	101:112	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	2	47	theme	major	190:194	arg1	byproduct					196:204	a major byproduct	188:204	a major byproduct worldwide	188:214	Grape pomace (GP) is a major byproduct worldwide, and it is well known for its bioactive compounds, such as fibers and phenolic compounds, that are popular for their impact upon human health, including gastrointestinal health.					
30950465	11	48	theme	food	1949:1952	arg1	industry					1954:1961	the food industry	1945:1961	the food industry such that it can be used as an ingredient in the development of new functional foods	1945:2046	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	6	49	theme	dietary	958:964	arg1	fiber					966:970	dietary fiber	958:970	dietary fiber	958:970	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	7	50	theme	phenolic	1168:1175	arg1	compounds					1177:1185	phenolic compounds	1168:1185	phenolic compounds	1168:1185	In vitro simulated GID revealed that xylobiose was resistant to gastric conditions, unlike phenolic compounds.					
30950465	3	51	theme	gastrointestinal	546:561	arg1	GID					574:576	GID	574:576	GID	574:576	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	3	51	theme	gastrointestinal	546:561	arg1	digestion					563:571	gastrointestinal digestion	546:571	gastrointestinal digestion (GID)	546:577	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	8	52	theme	ingredient	1216:1225	arg1	w/v					1203:1205	w/v	1203:1205	w/v	1203:1205	The use of 2% (w/v) of this ingredient proved to be a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion.					
30950465	8	52	theme	ingredient	1216:1225	arg1	%					1200:1200	2%	1199:1200	2% (w/v) of this ingredient	1199:1225	The use of 2% (w/v) of this ingredient proved to be a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion.					
30950465	8	52	theme	ingredient	1216:1225	arg1	ingredient					1216:1225	this ingredient	1211:1225	this ingredient	1211:1225	The use of 2% (w/v) of this ingredient proved to be a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion.					
30950465	7	53	theme	In	1077:1078	arg1	GID					1096:1098	In vitro simulated GID	1077:1098	In vitro simulated GID	1077:1098	In vitro simulated GID revealed that xylobiose was resistant to gastric conditions, unlike phenolic compounds.					
30950465	6	54	theme	phenolic	1057:1064	arg1	compounds					1066:1074	phenolic compounds	1057:1074	phenolic compounds	1057:1074	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	10	55	theme	intestinal	1685:1694	arg1	cells					1696:1700	Caco-2 intestinal cells	1678:1700	Caco-2 intestinal cells	1678:1700	Furthermore, the extract safety was also guaranteed on Caco-2 intestinal cells.					
30950465	4	56	with	cocktail	661:668	arg1	activity					684:691	xylanase activity	675:691	xylanase activity	675:691	GP extract was previously produced using an enzymatic cocktail with xylanase activity and was then exposed to simulated conditions of GID, characterized for its chemical composition, and screened for antimicrobial, prebiotic, and antioxidant activities.					
30950465	9	57	theme	antioxidant	1530:1540	arg1	capacity					1542:1549	the antioxidant capacity	1526:1549	the antioxidant capacity	1526:1549	The extract also exhibited strong antioxidant and antimicrobial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa; however, after GID, the antioxidant capacity decreased, and the antimicrobial capacity was strongly reduced or lost.					
30950465	4	58	theme	simulated	717:725	arg1	conditions					727:736	simulated conditions	717:736	simulated conditions of GID	717:743	GP extract was previously produced using an enzymatic cocktail with xylanase activity and was then exposed to simulated conditions of GID, characterized for its chemical composition, and screened for antimicrobial, prebiotic, and antioxidant activities.					
30950465	4	59	theme	GID	741:743	arg1	conditions					727:736	simulated conditions	717:736	simulated conditions of GID	717:743	GP extract was previously produced using an enzymatic cocktail with xylanase activity and was then exposed to simulated conditions of GID, characterized for its chemical composition, and screened for antimicrobial, prebiotic, and antioxidant activities.					
30950465	4	60	theme	enzymatic	651:659	arg1	cocktail					661:668	an enzymatic cocktail	648:668	an enzymatic cocktail with xylanase activity	648:691	GP extract was previously produced using an enzymatic cocktail with xylanase activity and was then exposed to simulated conditions of GID, characterized for its chemical composition, and screened for antimicrobial, prebiotic, and antioxidant activities.					
30950465	11	61	theme	potential	1919:1927	arg1	applications					1929:1940	potential applications	1919:1940	potential applications	1919:1940	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	8	62	theme	carbon	1252:1257	arg1	source					1259:1264	a potential carbon source	1240:1264	a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion	1240:1351	The use of 2% (w/v) of this ingredient proved to be a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion.					
30950465	8	62	theme	carbon	1252:1257	arg1	use					1192:1194	The use	1188:1194	The use of 2% (w/v) of this ingredient	1188:1225	The use of 2% (w/v) of this ingredient proved to be a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion.					
30950465	0	63	dep	in	10:11	arg1	vitro					13:17	vitro	13:17	vitro	13:17	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	0	63	dep	in	10:11	arg1	gastrointestinal					19:34	gastrointestinal	19:34	gastrointestinal	19:34	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	4	64	theme	xylanase	675:682	arg1	activity					684:691	xylanase activity	675:691	xylanase activity	675:691	GP extract was previously produced using an enzymatic cocktail with xylanase activity and was then exposed to simulated conditions of GID, characterized for its chemical composition, and screened for antimicrobial, prebiotic, and antioxidant activities.					
30950465	3	65	theme	work	416:419	arg1	objective					398:406	The objective	394:406	The objective of this work	394:419	The objective of this work was to evaluate the chemical composition and biological activities of an enzymatic GP extract, as well as to investigate how gastrointestinal digestion (GID) modulates these properties.					
30950465	9	66	theme	antioxidant	1388:1398	arg1	activities					1418:1427	strong antioxidant and antimicrobial activities	1381:1427	strong antioxidant and antimicrobial activities	1381:1427	The extract also exhibited strong antioxidant and antimicrobial activities against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa; however, after GID, the antioxidant capacity decreased, and the antimicrobial capacity was strongly reduced or lost.					
30950465	8	67	theme	%	1200:1200	arg1	use					1192:1194	The use	1188:1194	The use of 2% (w/v) of this ingredient	1188:1225	The use of 2% (w/v) of this ingredient proved to be a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion.					
30950465	8	67	theme	%	1200:1200	arg1	source					1259:1264	a potential carbon source	1240:1264	a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion	1240:1351	The use of 2% (w/v) of this ingredient proved to be a potential carbon source that could be fermented by Lactobacillus and Bifidobacterium spp, even after digestion.					
30950465	11	68	theme	biological	1889:1898	arg1	properties					1900:1909	different biological properties	1879:1909	different biological properties	1879:1909	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	0	69	dep	L.	132:133	arg1	Impact					0:5	Impact	0:5	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera	0:130	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	6	70	theme	high	941:944	arg1	contents					946:953	high contents	941:953	high contents of dietary fiber	941:970	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	6	70	theme	high	941:944	arg1	xylooligosaccharides					1007:1026	xylooligosaccharides	1007:1026	xylooligosaccharides	1007:1026	GP extract presented high contents of dietary fiber and other carbohydrates, including xylooligosaccharides, in addition to minerals and phenolic compounds.					
30950465	0	71	theme	chemical	53:60	arg1	composition					62:72	the chemical composition	49:72	the chemical composition	49:72	Impact of in vitro gastrointestinal digestion on the chemical composition, bioactive properties, and cytotoxicity of Vitis vinifera L. cv.					
30950465	11	72	from	ingredient	1994:2003	arg1	development					2012:2022	the development	2008:2022	the development of new functional foods	2008:2046	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	4	73	theme	GP	607:608	arg1	extract					610:616	GP extract	607:616	GP extract	607:616	GP extract was previously produced using an enzymatic cocktail with xylanase activity and was then exposed to simulated conditions of GID, characterized for its chemical composition, and screened for antimicrobial, prebiotic, and antioxidant activities.					
30950465	11	74	contain	has	1915:1917	arg2	applications					1929:1940	potential applications	1919:1940	potential applications	1919:1940	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30950465	11	74	contain	has	1915:1917	arg1	it					1912:1913	it	1912:1913	it	1912:1913	This novel and green GP extract proved to be composed of relevant bioactive molecules, including xylooligosaccharides, polyphenols, organic acids, and minerals, which provided different biological properties; it has potential applications in the food industry such that it can be used as an ingredient in the development of new functional foods.					
30004630	4	0	theme	week	732:735	arg1	intervention					737:748	a 4 week intervention	728:748	a 4 week intervention	728:748	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	0	1	dep	Microbiome	82:91	arg1	the					74:76	the	74:76	the	74:76	Ingestion of an Inulin-Enriched Pork Sausage Product Positively Modulates the Gut Microbiome and Metabolome of Healthy Rats.					
30004630	3	2	theme	healthy	499:505	arg1	rats					507:510	healthy rats	499:510	healthy rats	499:510	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	9	3	theme	fiber	1295:1299	arg1	consumption					1301:1311	dietary fiber consumption	1287:1311	dietary fiber consumption	1287:1311	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	2	4	theme	CRC	287:289	arg1	risk					291:294	CRC risk	287:294	CRC risk	287:294	In contrast, dietary fiber consumption has been found to lower CRC risk, possibly via mechanisms involving the gut microbiota (GM) and its metabolites.					
30004630	1	5	theme	increased	184:192	arg1	risk					218:221	a potential increased colorectal cancer (CRC) risk	172:221	a potential increased colorectal cancer (CRC) risk	172:221	SCOPE Processed meat intake is associated with a potential increased colorectal cancer (CRC) risk.					
30004630	0	6	theme	Rats	119:122	arg1	Metabolome					97:106	Metabolome	97:106	Metabolome	97:106	Ingestion of an Inulin-Enriched Pork Sausage Product Positively Modulates the Gut Microbiome and Metabolome of Healthy Rats.					
30004630	0	6	theme	Rats	119:122	arg1	Microbiome					82:91	Gut Microbiome	78:91	Gut Microbiome	78:91	Ingestion of an Inulin-Enriched Pork Sausage Product Positively Modulates the Gut Microbiome and Metabolome of Healthy Rats.					
30004630	9	7	theme	processed	1345:1353	arg1	meats					1355:1359	healthier processed meats	1335:1359	healthier processed meats	1335:1359	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	7	8	theme	short	1033:1037	arg1	SCFAs					1058:1062	SCFAs	1058:1062	SCFAs	1058:1062	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	8	theme	short	1033:1037	arg1	acids					1051:1055	short chain fatty acids	1033:1055	short chain fatty acids (SCFAs) in the fecal and plasma metabolome	1033:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	4	9	theme	corresponding	630:642	arg1	sausage					652:658	a corresponding control sausage	628:658	a corresponding control sausage without enrichment (n = 12)	628:686	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	2	10	dep	risk	291:294	arg1	lower					281:285	lower	281:285	lower	281:285	In contrast, dietary fiber consumption has been found to lower CRC risk, possibly via mechanisms involving the gut microbiota (GM) and its metabolites.					
30004630	2	11	theme	dietary	237:243	arg1	consumption					251:261	dietary fiber consumption	237:261	dietary fiber consumption	237:261	In contrast, dietary fiber consumption has been found to lower CRC risk, possibly via mechanisms involving the gut microbiota (GM) and its metabolites.					
30004630	9	12	theme	strategic	1386:1394	arg1	inclusion					1396:1404	strategic inclusion	1386:1404	strategic inclusion of dietary fiber ingredients	1386:1433	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	7	13	theme	plasma	1082:1087	arg1	metabolome					1089:1098	the fecal and plasma metabolome	1068:1098	metabolome	1089:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	5	14	theme	metabolomics	761:772	arg1	analyses					774:781	NMR-based metabolomics analyses	751:781	NMR-based metabolomics analyses	751:781	NMR-based metabolomics analyses are conducted on fecal and plasma samples, and GM composition is determined using 16S rRNA gene amplicon sequencing.					
30004630	3	15	from	activity	487:494	arg1	rats					507:510	healthy rats	499:510	healthy rats	499:510	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	0	16	theme	Gut	78:80	arg1	Microbiome					82:91	Gut Microbiome	78:91	Gut Microbiome	78:91	Ingestion of an Inulin-Enriched Pork Sausage Product Positively Modulates the Gut Microbiome and Metabolome of Healthy Rats.					
30004630	9	17	theme	fiber	1417:1421	arg1	ingredients					1423:1433	dietary fiber ingredients	1409:1433	dietary fiber ingredients	1409:1433	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	1	18	theme	Processed	131:139	arg1	intake					146:151	SCOPE Processed meat intake	125:151	SCOPE Processed meat intake	125:151	SCOPE Processed meat intake is associated with a potential increased colorectal cancer (CRC) risk.					
30004630	7	19	from	acids	1051:1055	arg1	metabolome					1089:1098	the fecal and plasma metabolome	1068:1098	metabolome	1089:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	20	theme	acids	1051:1055	arg1	levels					1023:1028	increased levels	1013:1028	increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome	1013:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	20	theme	acids	1051:1055	arg1	levels					1120:1125	increased fecal levels	1104:1125	increased fecal levels of Bifidobacterium spp	1104:1148	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	6	21	theme	GM	931:932	arg1	composition					934:944	GM composition	931:944	GM composition	931:944	Pronounced effects of diets on GM composition and activity are found.					
30004630	3	22	theme	enrichment	421:430	arg1	effect					404:409	the effect	400:409	the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats	400:510	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	7	23	theme	chain	1039:1043	arg1	SCFAs					1058:1062	SCFAs	1058:1062	SCFAs	1058:1062	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	23	theme	chain	1039:1043	arg1	acids					1051:1055	short chain fatty acids	1033:1055	short chain fatty acids (SCFAs) in the fecal and plasma metabolome	1033:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	9	24	theme	CONCLUSION	1204:1213	arg1	enrichment					1222:1231	CONCLUSION Inulin enrichment	1204:1231	CONCLUSION Inulin enrichment of a meat product	1204:1249	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	6	25	theme	diets	922:926	arg1	effects					911:917	Pronounced effects	900:917	Pronounced effects of diets on GM composition and activity	900:957	Pronounced effects of diets on GM composition and activity are found.					
30004630	0	26	theme	Pork	32:35	arg1	Product					45:51	an Inulin-Enriched Pork Sausage Product	13:51	an Inulin-Enriched Pork Sausage Product	13:51	Ingestion of an Inulin-Enriched Pork Sausage Product Positively Modulates the Gut Microbiome and Metabolome of Healthy Rats.					
30004630	2	27	located	found	272:276	arg2	consumption					251:261	dietary fiber consumption	237:261	dietary fiber consumption	237:261	In contrast, dietary fiber consumption has been found to lower CRC risk, possibly via mechanisms involving the gut microbiota (GM) and its metabolites.					
30004630	2	27	located	found	272:276	arg1	contrast					227:234	contrast	227:234	contrast	227:234	In contrast, dietary fiber consumption has been found to lower CRC risk, possibly via mechanisms involving the gut microbiota (GM) and its metabolites.					
30004630	4	28	theme	=	716:716	arg1	n					714:714	n = 6	714:718	n = 6	714:718	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	4	28	theme	=	716:716	arg1	diet					708:711	a standard chow diet	692:711	a standard chow diet (n = 6)	692:719	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	3	29	theme	pork	444:447	arg1	product					457:463	a common pork sausage product	435:463	a common pork sausage product	435:463	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	0	30	theme	Product	45:51	arg1	Ingestion					0:8	Ingestion	0:8	Ingestion of an Inulin-Enriched Pork Sausage Product	0:51	Ingestion of an Inulin-Enriched Pork Sausage Product Positively Modulates the Gut Microbiome and Metabolome of Healthy Rats.					
30004630	7	31	theme	spp	1146:1148	arg1	levels					1023:1028	increased levels	1013:1028	increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome	1013:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	31	theme	spp	1146:1148	arg1	levels					1120:1125	increased fecal levels	1104:1125	increased fecal levels of Bifidobacterium spp	1104:1148	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	9	32	theme	product	1243:1249	arg1	enrichment					1222:1231	CONCLUSION Inulin enrichment	1204:1231	CONCLUSION Inulin enrichment of a meat product	1204:1249	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	5	33	theme	16S	865:867	arg1	sequencing					888:897	16S rRNA gene amplicon sequencing	865:897	16S rRNA gene amplicon sequencing	865:897	NMR-based metabolomics analyses are conducted on fecal and plasma samples, and GM composition is determined using 16S rRNA gene amplicon sequencing.					
30004630	4	34	theme	chow	703:706	arg1	n					714:714	n = 6	714:718	n = 6	714:718	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	4	34	theme	chow	703:706	arg1	diet					708:711	a standard chow diet	692:711	a standard chow diet (n = 6)	692:719	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	3	35	theme	GM	468:469	arg1	composition					471:481	GM composition	468:481	GM composition	468:481	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	9	36	theme	general	1261:1267	arg1	effects					1269:1275	general effects	1261:1275	general effects seen upon dietary fiber consumption	1261:1311	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	5	37	theme	gene	874:877	arg1	sequencing					888:897	16S rRNA gene amplicon sequencing	865:897	16S rRNA gene amplicon sequencing	865:897	NMR-based metabolomics analyses are conducted on fecal and plasma samples, and GM composition is determined using 16S rRNA gene amplicon sequencing.					
30004630	7	38	theme	fecal	1114:1118	arg1	levels					1120:1125	increased fecal levels	1104:1125	increased fecal levels of Bifidobacterium spp	1104:1148	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	39	theme	inulin-enriched	983:997	arg1	sausages					999:1006	the inulin-enriched sausages	979:1006	the inulin-enriched sausages	979:1006	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	3	40	from	effect	404:409	arg1	activity					487:494	activity	487:494	activity	487:494	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	3	40	from	effect	404:409	arg1	composition					471:481	GM composition	468:481	GM composition	468:481	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	3	41	theme	product	457:463	arg1	enrichment					421:430	inulin enrichment	414:430	inulin enrichment of a common pork sausage product	414:463	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	4	42	theme	4	730:730	arg1	week					732:735	week	732:735	week	732:735	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	7	43	from	metabolome	1089:1098	arg1	levels					1023:1028	increased levels	1013:1028	increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome	1013:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	43	from	metabolome	1089:1098	arg1	levels					1120:1125	increased fecal levels	1104:1125	increased fecal levels of Bifidobacterium spp	1104:1148	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	44	contain	have	1008:1011	arg1	Rats					970:973	Rats	970:973	Rats fed the inulin-enriched sausages	970:1006	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	44	contain	have	1008:1011	arg2	levels					1120:1125	increased fecal levels	1104:1125	increased fecal levels of Bifidobacterium spp	1104:1148	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	44	contain	have	1008:1011	arg2	levels					1023:1028	increased levels	1013:1028	increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome	1013:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	9	45	theme	dietary	1287:1293	arg1	consumption					1301:1311	dietary fiber consumption	1287:1311	dietary fiber consumption	1287:1311	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	4	46	theme	Sprague-Dawley	540:553	arg1	rats					555:558	Thirty Sprague-Dawley rats	533:558	METHODS AND RESULTS Thirty Sprague-Dawley rats	513:558	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	3	47	from	composition	471:481	arg1	rats					507:510	healthy rats	499:510	healthy rats	499:510	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	7	48	theme	increased	1013:1021	arg1	levels					1023:1028	increased levels	1013:1028	increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome	1013:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	49	from	levels	1120:1125	arg1	metabolome					1089:1098	the fecal and plasma metabolome	1068:1098	metabolome	1089:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	4	50	theme	inulin-enriched	594:608	arg1	sausage					610:616	an inulin-enriched sausage	591:616	either an inulin-enriched sausage (n = 12)	584:625	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	4	50	theme	inulin-enriched	594:608	arg1	n					619:619	n = 12	619:624	n = 12	619:624	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	7	51	theme	fecal	1072:1076	arg1	metabolome					1089:1098	the fecal and plasma metabolome	1068:1098	metabolome	1089:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	6	52	from	effects	911:917	arg1	composition					934:944	GM composition	931:944	GM composition	931:944	Pronounced effects of diets on GM composition and activity are found.					
30004630	6	52	from	effects	911:917	arg1	activity					950:957	activity	950:957	activity	950:957	Pronounced effects of diets on GM composition and activity are found.					
30004630	1	53	theme	potential	174:182	arg1	risk					218:221	a potential increased colorectal cancer (CRC) risk	172:221	a potential increased colorectal cancer (CRC) risk	172:221	SCOPE Processed meat intake is associated with a potential increased colorectal cancer (CRC) risk.					
30004630	1	54	theme	colorectal	194:203	arg1	cancer					205:210	colorectal cancer	194:210	a potential increased colorectal cancer (CRC) risk	172:221	SCOPE Processed meat intake is associated with a potential increased colorectal cancer (CRC) risk.					
30004630	1	54	theme	colorectal	194:203	arg1	CRC					213:215	CRC	213:215	CRC	213:215	SCOPE Processed meat intake is associated with a potential increased colorectal cancer (CRC) risk.					
30004630	1	55	theme	meat	141:144	arg1	intake					146:151	SCOPE Processed meat intake	125:151	SCOPE Processed meat intake	125:151	SCOPE Processed meat intake is associated with a potential increased colorectal cancer (CRC) risk.					
30004630	9	56	dep	processed	1345:1353	arg1	healthier					1335:1343	healthier	1335:1343	healthier	1335:1343	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	2	57	theme	fiber	245:249	arg1	consumption					251:261	dietary fiber consumption	237:261	dietary fiber consumption	237:261	In contrast, dietary fiber consumption has been found to lower CRC risk, possibly via mechanisms involving the gut microbiota (GM) and its metabolites.					
30004630	6	58	theme	Pronounced	900:909	arg1	effects					911:917	Pronounced effects	900:917	Pronounced effects of diets on GM composition and activity	900:957	Pronounced effects of diets on GM composition and activity are found.					
30004630	9	59	theme	ingredients	1423:1433	arg1	inclusion					1396:1404	strategic inclusion	1386:1404	strategic inclusion of dietary fiber ingredients	1386:1433	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	4	60	theme	control	644:650	arg1	sausage					652:658	a corresponding control sausage	628:658	a corresponding control sausage without enrichment (n = 12)	628:686	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	5	61	theme	NMR-based	751:759	arg1	analyses					774:781	NMR-based metabolomics analyses	751:781	NMR-based metabolomics analyses	751:781	NMR-based metabolomics analyses are conducted on fecal and plasma samples, and GM composition is determined using 16S rRNA gene amplicon sequencing.					
30004630	4	62	theme	=	621:621	arg1	sausage					610:616	an inulin-enriched sausage	591:616	either an inulin-enriched sausage (n = 12)	584:625	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	4	62	theme	=	621:621	arg1	n					619:619	n = 12	619:624	n = 12	619:624	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	9	63	theme	dietary	1409:1415	arg1	ingredients					1423:1433	dietary fiber ingredients	1409:1433	dietary fiber ingredients	1409:1433	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	7	64	from	levels	1023:1028	arg1	metabolome					1089:1098	the fecal and plasma metabolome	1068:1098	metabolome	1089:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	5	65	theme	GM	830:831	arg1	composition					833:843	GM composition	830:843	GM composition	830:843	NMR-based metabolomics analyses are conducted on fecal and plasma samples, and GM composition is determined using 16S rRNA gene amplicon sequencing.					
30004630	4	66	theme	=	682:682	arg1	n					680:680	n = 12	680:685	n = 12	680:685	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	4	66	theme	=	682:682	arg1	enrichment					668:677	enrichment	668:677	enrichment (n = 12)	668:686	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	1	67	dep	Processed	131:139	arg1	SCOPE					125:129	SCOPE	125:129	SCOPE	125:129	SCOPE Processed meat intake is associated with a potential increased colorectal cancer (CRC) risk.					
30004630	0	68	theme	Healthy	111:117	arg1	Rats					119:122	Healthy Rats	111:122	Healthy Rats	111:122	Ingestion of an Inulin-Enriched Pork Sausage Product Positively Modulates the Gut Microbiome and Metabolome of Healthy Rats.					
30004630	4	69	dep	METHODS	513:519	arg1	rats					555:558	Thirty Sprague-Dawley rats	533:558	METHODS AND RESULTS Thirty Sprague-Dawley rats	513:558	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	1	70	theme	cancer	205:210	arg1	risk					218:221	a potential increased colorectal cancer (CRC) risk	172:221	a potential increased colorectal cancer (CRC) risk	172:221	SCOPE Processed meat intake is associated with a potential increased colorectal cancer (CRC) risk.					
30004630	7	71	theme	fatty	1045:1049	arg1	SCFAs					1058:1062	SCFAs	1058:1062	SCFAs	1058:1062	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	7	71	theme	fatty	1045:1049	arg1	acids					1051:1055	short chain fatty acids	1033:1055	short chain fatty acids (SCFAs) in the fecal and plasma metabolome	1033:1098	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	5	72	theme	plasma	810:815	arg1	samples					817:823	plasma samples	810:823	plasma samples	810:823	NMR-based metabolomics analyses are conducted on fecal and plasma samples, and GM composition is determined using 16S rRNA gene amplicon sequencing.					
30004630	0	73	theme	Inulin-Enriched	16:30	arg1	Product					45:51	an Inulin-Enriched Pork Sausage Product	13:51	an Inulin-Enriched Pork Sausage Product	13:51	Ingestion of an Inulin-Enriched Pork Sausage Product Positively Modulates the Gut Microbiome and Metabolome of Healthy Rats.					
30004630	4	74	dep	sausage	610:616	arg1	either					584:589	either	584:589	either	584:589	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	0	75	theme	Sausage	37:43	arg1	Product					45:51	an Inulin-Enriched Pork Sausage Product	13:51	an Inulin-Enriched Pork Sausage Product	13:51	Ingestion of an Inulin-Enriched Pork Sausage Product Positively Modulates the Gut Microbiome and Metabolome of Healthy Rats.					
30004630	9	76	theme	Inulin	1215:1220	arg1	enrichment					1222:1231	CONCLUSION Inulin enrichment	1204:1231	CONCLUSION Inulin enrichment of a meat product	1204:1249	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	5	77	theme	amplicon	879:886	arg1	sequencing					888:897	16S rRNA gene amplicon sequencing	865:897	16S rRNA gene amplicon sequencing	865:897	NMR-based metabolomics analyses are conducted on fecal and plasma samples, and GM composition is determined using 16S rRNA gene amplicon sequencing.					
30004630	3	78	theme	inulin	414:419	arg1	enrichment					421:430	inulin enrichment	414:430	inulin enrichment of a common pork sausage product	414:463	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	7	79	theme	Bifidobacterium	1130:1144	arg1	spp					1146:1148	Bifidobacterium spp	1130:1148	Bifidobacterium spp	1130:1148	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	3	80	theme	sausage	449:455	arg1	product					457:463	a common pork sausage product	435:463	a common pork sausage product	435:463	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	9	81	theme	meat	1238:1241	arg1	product					1243:1249	a meat product	1236:1249	a meat product	1236:1249	CONCLUSION Inulin enrichment of a meat product resembles general effects seen upon dietary fiber consumption and corroborates that healthier processed meats can be developed through strategic inclusion of dietary fiber ingredients.					
30004630	3	82	theme	common	437:442	arg1	product					457:463	a common pork sausage product	435:463	a common pork sausage product	435:463	This study investigates the effect of inulin enrichment of a common pork sausage product on GM composition and activity in healthy rats.					
30004630	5	83	theme	rRNA	869:872	arg1	sequencing					888:897	16S rRNA gene amplicon sequencing	865:897	16S rRNA gene amplicon sequencing	865:897	NMR-based metabolomics analyses are conducted on fecal and plasma samples, and GM composition is determined using 16S rRNA gene amplicon sequencing.					
30004630	7	84	theme	increased	1104:1112	arg1	levels					1120:1125	increased fecal levels	1104:1125	increased fecal levels of Bifidobacterium spp	1104:1148	Rats fed the inulin-enriched sausages have increased levels of short chain fatty acids (SCFAs) in the fecal and plasma metabolome and increased fecal levels of Bifidobacterium spp.					
30004630	4	85	theme	standard	694:701	arg1	n					714:714	n = 6	714:718	n = 6	714:718	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	4	85	theme	standard	694:701	arg1	diet					708:711	a standard chow diet	692:711	a standard chow diet (n = 6)	692:719	METHODS AND RESULTS Thirty Sprague-Dawley rats are fed a diet based on either an inulin-enriched sausage (n = 12), a corresponding control sausage without enrichment (n = 12), or a standard chow diet (n = 6) during a 4 week intervention.					
30004630	2	86	theme	gut	335:337	arg1	GM					351:352	GM	351:352	GM	351:352	In contrast, dietary fiber consumption has been found to lower CRC risk, possibly via mechanisms involving the gut microbiota (GM) and its metabolites.					
30004630	2	86	theme	gut	335:337	arg1	microbiota					339:348	the gut microbiota	331:348	the gut microbiota (GM)	331:353	In contrast, dietary fiber consumption has been found to lower CRC risk, possibly via mechanisms involving the gut microbiota (GM) and its metabolites.					
31523960	5	0	from	increase	1103:1110	arg1	families					1184:1191	the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families	1115:1191	the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families	1115:1191	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	2	1	dep	metabolites	448:458	arg1	ethylcoprostanol					511:526	ethylcoprostanol	511:526	ethylcoprostanol	511:526	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	1	dep	metabolites	448:458	arg1	methylcoprostanol					474:490	methylcoprostanol	474:490	methylcoprostanol	474:490	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	1	dep	metabolites	448:458	arg1	sitostenone					533:543	sitostenone	533:543	sitostenone	533:543	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	1	dep	metabolites	448:458	arg1	ethylcoprostenol					493:508	ethylcoprostenol	493:508	ethylcoprostenol	493:508	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	1	dep	metabolites	448:458	arg1	metabolites					448:458	sterol metabolites	441:458	sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone)	441:544	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	1	dep	metabolites	448:458	arg1	coprostanol					461:471	coprostanol	461:471	coprostanol	461:471	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	3	2	theme	Microbial	666:674	arg1	activity					676:683	Microbial activity	666:683	Microbial activity	666:683	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	0	3	dep	In	133:134	arg1	Vitro					136:140	Vitro	136:140	Vitro	136:140	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	1	4	theme	1.8	238:240	arg1	mL					248:249	mL	248:249	mL	248:249	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	5	5	theme	galactooligosaccharides	1023:1045	arg1	effect					1013:1018	the prebiotic effect	999:1018	the prebiotic effect of galactooligosaccharides	999:1045	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	5	6	from	changes	1065:1071	arg1	composition					1087:1097	microbiota composition	1076:1097	microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families)	1076:1192	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	5	7	theme	prebiotic	1003:1011	arg1	effect					1013:1018	the prebiotic effect	999:1018	the prebiotic effect of galactooligosaccharides	999:1045	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	1	8	theme	g/100	242:246	arg1	mL					248:249	mL	248:249	mL	248:249	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	0	9	theme	Microbiota	101:110	arg1	Composition					112:122	Gut Microbiota Composition	97:122	Gut Microbiota Composition	97:122	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	0	10	from	Impact	0:5	arg1	Metabolism					82:91	Colonic Metabolism	74:91	Colonic Metabolism	74:91	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	0	10	from	Impact	0:5	arg1	Composition					112:122	Gut Microbiota Composition	97:122	Gut Microbiota Composition	97:122	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	4	11	dep	TC	904:905	arg1	the					900:902	the	900:902	the	900:902	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	4	12	theme	sterol	943:948	arg1	metabolism					950:959	a higher sterol metabolism	934:959	a higher sterol metabolism	934:959	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	3	13	theme	colon	761:765	arg1	vessels					767:773	all colon vessels	757:773	all colon vessels	757:773	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	0	14	theme	Gut	97:99	arg1	Composition					112:122	Gut Microbiota Composition	97:122	Gut Microbiota Composition	97:122	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	3	15	from	butyrate	799:806	arg1	AC					791:792	the AC	787:792	the AC	787:792	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	3	15	from	butyrate	799:806	arg1	vessels					767:773	all colon vessels	757:773	all colon vessels	757:773	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	4	16	from	diversity	850:858	arg1	community					877:885	the microbial community	863:885	the microbial community	863:885	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	3	17	theme	acids	732:736	arg1	production					710:719	the production	706:719	the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC	706:838	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	1	18	theme	enriched	168:175	arg1	beverage					159:166	A beverage	157:166	A beverage enriched with plant sterols (1 g/100 mL)	157:207	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	2	19	theme	sterol	441:446	arg1	ethylcoprostanol					511:526	ethylcoprostanol	511:526	ethylcoprostanol	511:526	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	19	theme	sterol	441:446	arg1	methylcoprostanol					474:490	methylcoprostanol	474:490	methylcoprostanol	474:490	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	19	theme	sterol	441:446	arg1	sitostenone					533:543	sitostenone	533:543	sitostenone	533:543	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	19	theme	sterol	441:446	arg1	ethylcoprostenol					493:508	ethylcoprostenol	493:508	ethylcoprostenol	493:508	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	19	theme	sterol	441:446	arg1	metabolites					448:458	sterol metabolites	441:458	sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone)	441:544	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	19	theme	sterol	441:446	arg1	coprostanol					461:471	coprostanol	461:471	coprostanol	461:471	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	3	20	from	propionate	812:821	arg1	AC					791:792	the AC	787:792	the AC	787:792	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	3	20	from	propionate	812:821	arg1	vessels					767:773	all colon vessels	757:773	all colon vessels	757:773	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	4	21	theme	microbial	867:875	arg1	community					877:885	the microbial community	863:885	the microbial community	863:885	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	1	22	theme	sterol	355:360	arg1	metabolism					362:371	sterol metabolism	355:371	sterol metabolism	355:371	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	2	23	theme	colon	603:607	arg1	vessels					614:620	the transverse colon (TC) and descending colon (DC) vessels	562:620	the transverse colon (TC) and descending colon (DC) vessels in general	562:631	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	3	24	from	acetate	746:752	arg1	AC					791:792	the AC	787:792	the AC	787:792	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	3	24	from	acetate	746:752	arg1	vessels					767:773	all colon vessels	757:773	all colon vessels	757:773	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	1	25	theme	plant	182:186	arg1	sterols					188:194	plant sterols	182:194	plant sterols	182:194	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	4	26	theme	higher	843:848	arg1	diversity					850:858	A higher diversity	841:858	A higher diversity in the microbial community	841:885	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	2	27	theme	descending	592:601	arg1	colon					603:607	descending colon	592:607	descending colon (DC)	592:612	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	27	theme	descending	592:601	arg1	DC					610:611	DC	610:611	DC	610:611	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	5	28	dep	Synergistaceae	1149:1162	arg1	the					1145:1147	the	1145:1147	the	1145:1147	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	5	29	dep	composition	1087:1097	arg1	increase					1103:1110	an increase	1100:1110	an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families	1100:1191	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	0	30	theme	Plant	12:16	arg1	Beverage					62:69	a Plant Sterol- and Galactooligosaccharide-Enriched Beverage	10:69	a Plant Sterol- and Galactooligosaccharide-Enriched Beverage	10:69	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	5	31	theme	microbiota	1076:1085	arg1	composition					1087:1097	microbiota composition	1076:1097	microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families)	1076:1192	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	0	32	theme	In	133:134	arg1	Model					150:154	an In Vitro Dynamic Model	130:154	an In Vitro Dynamic Model	130:154	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	2	33	located	observed	550:557	arg1	vessels					614:620	the transverse colon (TC) and descending colon (DC) vessels	562:620	the transverse colon (TC) and descending colon (DC) vessels in general	562:631	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	33	located	observed	550:557	arg2	Production					427:436	Production	427:436	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone)	427:544	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	1	34	from	effect	345:350	arg1	metabolism					362:371	sterol metabolism	355:371	sterol metabolism	355:371	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	1	34	from	effect	345:350	arg1	composition					414:424	microbiota composition	403:424	microbiota composition	403:424	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	1	34	from	effect	345:350	arg1	production					387:396	organic acid production	374:396	organic acid production	374:396	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	1	35	dep	galactooligosaccharides	213:235	arg1	mL					248:249	mL	248:249	mL	248:249	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	5	36	theme	metabolism	1229:1238	arg1	enhancement					1207:1217	an enhancement	1204:1217	an enhancement of sterol metabolism	1204:1238	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	1	37	theme	1	197:197	arg1	mL					205:206	mL	205:206	mL	205:206	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	0	38	theme	Dynamic	142:148	arg1	Model					150:154	an In Vitro Dynamic Model	130:154	an In Vitro Dynamic Model	130:154	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	3	39	from	production	710:719	arg1	TC					830:831	TC	830:831	TC	830:831	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	3	39	from	production	710:719	arg1	DC					837:838	DC	837:838	DC	837:838	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	1	40	theme	g/100	199:203	arg1	mL					205:206	mL	205:206	mL	205:206	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	0	41	theme	Sterol-	18:24	arg1	Beverage					62:69	a Plant Sterol- and Galactooligosaccharide-Enriched Beverage	10:69	a Plant Sterol- and Galactooligosaccharide-Enriched Beverage	10:69	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	1	42	theme	dynamic	271:277	arg1	process					321:327	a dynamic gastrointestinal and colonic fermentation process	269:327	a dynamic gastrointestinal and colonic fermentation process	269:327	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	3	43	from	lactate	776:782	arg1	AC					791:792	the AC	787:792	the AC	787:792	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	3	43	from	lactate	776:782	arg1	vessels					767:773	all colon vessels	757:773	all colon vessels	757:773	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	1	44	theme	organic	374:380	arg1	production					387:396	organic acid production	374:396	organic acid production	374:396	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	3	45	theme	organic	724:730	arg1	butyrate					799:806	butyrate	799:806	butyrate	799:806	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	3	45	theme	organic	724:730	arg1	propionate					812:821	propionate	812:821	propionate	812:821	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	3	45	theme	organic	724:730	arg1	acids					732:736	organic acids	724:736	organic acids	724:736	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	3	45	theme	organic	724:730	arg1	acetate					746:752	acetate	746:752	acetate in all colon vessels	746:773	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	3	45	theme	organic	724:730	arg1	lactate					776:782	lactate	776:782	lactate in the AC	776:792	Microbial activity was assessed through the production of organic acids, mainly acetate in all colon vessels, lactate in the AC, and butyrate and propionate in the TC and DC.					
31523960	0	46	theme	Beverage	62:69	arg1	Impact					0:5	Impact	0:5	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition	0:122	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	1	47	theme	gastrointestinal	279:294	arg1	process					321:327	a dynamic gastrointestinal and colonic fermentation process	269:327	a dynamic gastrointestinal and colonic fermentation process	269:327	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	2	48	theme	colon	577:581	arg1	vessels					614:620	the transverse colon (TC) and descending colon (DC) vessels	562:620	the transverse colon (TC) and descending colon (DC) vessels in general	562:631	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	1	49	theme	acid	382:385	arg1	production					387:396	organic acid production	374:396	organic acid production	374:396	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	1	50	dep	enriched	168:175	arg1	mL					205:206	mL	205:206	mL	205:206	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	2	51	from	vessels	614:620	arg1	general					625:631	general	625:631	general	625:631	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	0	52	theme	Galactooligosaccharide-Enriched	30:60	arg1	Beverage					62:69	a Plant Sterol- and Galactooligosaccharide-Enriched Beverage	10:69	a Plant Sterol- and Galactooligosaccharide-Enriched Beverage	10:69	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	2	53	theme	transverse	566:575	arg1	TC					584:585	TC	584:585	TC	584:585	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	2	53	theme	transverse	566:575	arg1	colon					577:581	the transverse colon	562:581	the transverse colon (TC)	562:586	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	4	54	located	found	891:895	arg2	diversity					850:858	A higher diversity	841:858	A higher diversity in the microbial community	841:885	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	4	54	located	found	891:895	arg1	DC					911:912	DC	911:912	DC	911:912	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	4	54	located	found	891:895	arg1	TC					904:905	TC	904:905	TC	904:905	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	5	55	dep	Parabacteroides	1119:1133	arg1	genus					1135:1139	Parabacteroides genus and the Synergistaceae and Lachnospiraceae	1119:1182	genus	1135:1139	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	4	56	theme	organic	965:971	arg1	production					978:987	organic acid production	965:987	organic acid production	965:987	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	1	57	theme	colonic	300:306	arg1	process					321:327	a dynamic gastrointestinal and colonic fermentation process	269:327	a dynamic gastrointestinal and colonic fermentation process	269:327	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	1	58	with	enriched	168:175	arg1	sterols					188:194	plant sterols	182:194	plant sterols	182:194	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	5	59	theme	Parabacteroides	1119:1133	arg1	families					1184:1191	the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families	1115:1191	the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families	1115:1191	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	5	60	theme	sterol	1222:1227	arg1	metabolism					1229:1238	sterol metabolism	1222:1238	sterol metabolism	1222:1238	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	0	61	theme	Colonic	74:80	arg1	Metabolism					82:91	Colonic Metabolism	74:91	Colonic Metabolism	74:91	Impact of a Plant Sterol- and Galactooligosaccharide-Enriched Beverage on Colonic Metabolism and Gut Microbiota Composition Using an In Vitro Dynamic Model.					
31523960	1	62	theme	microbiota	403:412	arg1	composition					414:424	microbiota composition	403:424	microbiota composition	403:424	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	5	63	theme	Synergistaceae	1149:1162	arg1	families					1184:1191	the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families	1115:1191	the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families	1115:1191	Although the prebiotic effect of galactooligosaccharides was not detected, changes in microbiota composition (an increase in the Parabacteroides genus and the Synergistaceae and Lachnospiraceae families) indicated an enhancement of sterol metabolism.					
31523960	4	64	theme	acid	973:976	arg1	production					978:987	organic acid production	965:987	organic acid production	965:987	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
31523960	2	65	theme	metabolites	448:458	arg1	Production					427:436	Production	427:436	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone)	427:544	Production of sterol metabolites (coprostanol, methylcoprostanol, ethylcoprostenol, ethylcoprostanol, and sitostenone) was observed in the transverse colon (TC) and descending colon (DC) vessels in general, from 24 and 48 h, respectively.					
31523960	1	66	theme	fermentation	308:319	arg1	process					321:327	a dynamic gastrointestinal and colonic fermentation process	269:327	a dynamic gastrointestinal and colonic fermentation process	269:327	A beverage enriched with plant sterols (1 g/100 mL) and galactooligosaccharides (1.8 g/100 mL) was subjected to a dynamic gastrointestinal and colonic fermentation process to evaluate the effect on sterol metabolism, organic acid production, and microbiota composition.					
31523960	4	67	theme	higher	936:941	arg1	metabolism					950:959	a higher sterol metabolism	934:959	a higher sterol metabolism	934:959	A higher diversity in the microbial community was found in the TC and DC, in accordance with a higher sterol metabolism and organic acid production.					
30308252	8	0	dep	composition	2001:2011	arg1	the					1988:1990	the	1988:1990	the	1988:1990	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	7	1	theme	Transwell®	1806:1815	arg1	system					1817:1822	a Transwell® system	1804:1822	a Transwell® system	1804:1822	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	7	2	from	cells	1795:1799	arg1	system					1817:1822	a Transwell® system	1804:1822	a Transwell® system	1804:1822	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	4	3	theme	FITC-dextran	995:1006	arg1	nanoparticles					1043:1055	FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	995:1055	FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	995:1055	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	9	4	theme	promising	2126:2134	arg1	platform					2147:2154	a promising evaluation platform	2124:2154	a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases	2124:2250	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	6	5	theme	in	1449:1450	arg1	models					1458:1463	Current in vitro models	1441:1463	Current in vitro models of the gastric mucosa	1441:1485	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	1	6	theme	gastric	231:237	arg1	mucosa					239:244	the gastric mucosa	227:244	the gastric mucosa	227:244	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	5	7	dep	in	1280:1281	arg1	vitro					1283:1287	vitro	1283:1287	vitro	1283:1287	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	4	8	theme	200 nm	1012:1017	arg1	nanoparticles					1043:1055	FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	995:1055	FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	995:1055	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	5	9	theme	extracellular	1204:1216	arg1	ECM					1226:1228	ECM	1226:1228	ECM	1226:1228	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	5	9	theme	extracellular	1204:1216	arg1	matrix					1218:1223	a gastric extracellular matrix	1194:1223	a gastric extracellular matrix (ECM)	1194:1229	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	7	10	theme	cancer	1788:1793	arg1	cells					1795:1799	epithelial gastric cancer cells	1769:1799	epithelial gastric cancer cells	1769:1799	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	6	11	theme	cell	1516:1519	arg1	systems					1529:1535	simplistic 2D cell culture systems	1502:1535	simplistic 2D cell culture systems	1502:1535	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	5	12	theme	lamina	1160:1165	arg1	propria					1167:1173	the biomimetic lamina propria	1145:1173	the biomimetic lamina propria	1145:1173	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	3	13	theme	cellular	696:703	arg1	component					705:713	cellular component	696:713	cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier	696:892	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	5	14	theme	diseases	1404:1411	arg1	tool					1347:1350	a reliable evaluation tool	1325:1350	a reliable evaluation tool of innovative therapeutics	1325:1377	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	5	14	theme	diseases	1404:1411	arg1	diagnosis					1383:1391	diagnosis	1383:1391	diagnosis of gastric diseases	1383:1411	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	6	15	theme	simplistic	1502:1511	arg1	systems					1529:1535	simplistic 2D cell culture systems	1502:1535	simplistic 2D cell culture systems	1502:1535	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	9	16	theme	new	2185:2187	arg1	strategies					2189:2198	new strategies	2185:2198	new strategies for the treatment and diagnosis of gastric diseases	2185:2250	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	4	17	dep	integrity	899:907	arg1	The					895:897	The	895:897	The	895:897	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	1	18	theme	reliable	183:190	arg1	models					201:206	reliable in vitro models	183:206	reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined	183:294	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	3	19	theme	lamina	722:727	arg1	propria					729:735	the lamina propria	718:735	the lamina propria	718:735	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	6	20	theme	stomach	1577:1583	arg1	wall					1585:1588	the stomach wall	1573:1588	the stomach wall	1573:1588	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	6	21	theme	gastric	1659:1665	arg1	pathologies					1667:1677	gastric pathologies	1659:1677	gastric pathologies	1659:1677	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	9	22	theme	evaluation	2136:2145	arg1	platform					2147:2154	a promising evaluation platform	2124:2154	a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases	2124:2250	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	3	23	theme	normal	525:530	arg1	fibroblasts					540:550	normal stomach fibroblasts	525:550	normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert	525:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	7	24	theme	3D	1731:1732	arg1	hydrogel					1756:1763	a 3D RGD-modified alginate hydrogel	1729:1763	a 3D RGD-modified alginate hydrogel	1729:1763	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	3	25	theme	3D	566:567	arg1	hydrogel					591:598	a 3D RGD-modified alginate hydrogel	564:598	a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert	564:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	1	26	theme	therapeutics	123:134	arg1	field					97:101	The field	93:101	The field of stomach-directed therapeutics and diagnosis	93:148	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	3	27	theme	cell	800:803	arg1	MKN74					811:815	MKN74	811:815	MKN74	811:815	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	3	27	theme	cell	800:803	arg1	line					805:808	a moderately differentiated adenocarcinoma stomach cell line	749:808	a moderately differentiated adenocarcinoma stomach cell line (MKN74)	749:816	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	2	28	theme	inner	445:449	arg1	stratum					451:457	an inner stratum	442:457	an inner stratum of the mucosa	442:471	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	6	29	theme	new	1629:1631	arg1	approaches					1645:1654	new therapeutic approaches	1629:1654	new therapeutic approaches to gastric pathologies	1629:1677	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	5	30	theme	in	1280:1281	arg1	model					1302:1306	an in vitro permeability model	1277:1306	an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases	1277:1411	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	9	31	theme	gastric	2235:2241	arg1	diseases					2243:2250	gastric diseases	2235:2250	gastric diseases	2235:2250	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	0	32	theme	gastric	44:50	arg1	mucosa					52:57	gastric mucosa	44:57	gastric mucosa	44:57	Bioengineering a novel 3D in vitro model of gastric mucosa for stomach permeability studies.					
30308252	1	33	theme	diagnosis	140:148	arg1	field					97:101	The field	93:101	The field of stomach-directed therapeutics and diagnosis	93:148	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	4	34	theme	in	934:935	arg1	model					943:947	the in vitro model	930:947	the in vitro model	930:947	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	3	35	theme	adenocarcinoma	777:790	arg1	MKN74					811:815	MKN74	811:815	MKN74	811:815	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	3	35	theme	adenocarcinoma	777:790	arg1	line					805:808	a moderately differentiated adenocarcinoma stomach cell line	749:808	a moderately differentiated adenocarcinoma stomach cell line (MKN74)	749:816	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	2	36	theme	mucosa	466:471	arg1	stratum					451:457	an inner stratum	442:457	an inner stratum of the mucosa	442:471	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	0	37	theme	permeability	71:82	arg1	studies					84:90	stomach permeability studies	63:90	stomach permeability studies	63:90	Bioengineering a novel 3D in vitro model of gastric mucosa for stomach permeability studies.					
30308252	3	38	theme	physiological	846:858	arg1	conditions					860:869	the physiological conditions	842:869	the physiological conditions of the gastric barrier	842:892	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	8	39	theme	extracellular	2039:2051	arg1	matrix					2053:2058	the extracellular matrix	2035:2058	the extracellular matrix of gastric lamina propria	2035:2084	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	8	40	theme	cellular	1992:1999	arg1	composition					2001:2011	cellular composition	1992:2011	cellular composition	1992:2011	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	5	41	theme	Nanoparticle	1080:1091	arg1	transport					1093:1101	Nanoparticle transport	1080:1101	Nanoparticle transport	1080:1101	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	2	42	theme	monolayer	423:431	arg1	model					433:437	a conventional gastric monolayer model	400:437	a conventional gastric monolayer model	400:437	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	8	43	theme	lamina	2071:2076	arg1	propria					2078:2084	gastric lamina propria	2063:2084	gastric lamina propria	2063:2084	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	4	44	theme	permeability	971:982	arg1	studies					984:990	permeability studies	971:990	permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	971:1055	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	8	45	theme	3D	1957:1958	arg1	hydrogel					1960:1967	the cell-laden 3D hydrogel	1942:1967	the cell-laden 3D hydrogel	1942:1967	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	2	46	theme	3D	346:347	arg1	model					358:362	a rapid, complex, and innovative 3D in vitro model	313:362	a rapid, complex, and innovative 3D in vitro model of the gastric mucosa	313:384	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	6	47	theme	SIGNIFICANCE	1427:1438	arg1	STATEMENT					1414:1422	STATEMENT	1414:1422	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.	1414:1678	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	1	48	theme	gastric	252:258	arg1	cells					267:271	gastric cancer cells	252:271	gastric cancer cells	252:271	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	3	49	theme	gastric	878:884	arg1	barrier					886:892	the gastric barrier	874:892	the gastric barrier	874:892	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	5	50	theme	permeability	1289:1300	arg1	model					1302:1306	an in vitro permeability model	1277:1306	an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases	1277:1411	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	2	51	theme	gastric	371:377	arg1	mucosa					379:384	the gastric mucosa	367:384	the gastric mucosa	367:384	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	0	52	theme	novel	17:21	arg1	model					35:39	a novel 3D in vitro model	15:39	a novel 3D in vitro model of gastric mucosa for stomach permeability studies	15:90	Bioengineering a novel 3D in vitro model of gastric mucosa for stomach permeability studies.					
30308252	4	53	theme	model	943:947	arg1	integrity					899:907	integrity	899:907	integrity	899:907	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	4	53	theme	model	943:947	arg1	functionality					913:925	functionality	913:925	functionality	913:925	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	0	54	theme	in	26:27	arg1	model					35:39	a novel 3D in vitro model	15:39	a novel 3D in vitro model of gastric mucosa for stomach permeability studies	15:90	Bioengineering a novel 3D in vitro model of gastric mucosa for stomach permeability studies.					
30308252	7	55	theme	mucosa	1878:1883	arg1	model					1857:1861	a new biomimetic model	1840:1861	a new biomimetic model of the stomach mucosa	1840:1883	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	0	56	dep	model	35:39	arg1	Bioengineering					0:13	Bioengineering	0:13	Bioengineering	0:13	Bioengineering a novel 3D in vitro model of gastric mucosa for stomach permeability studies.					
30308252	6	57	dep	in	1449:1450	arg1	vitro					1452:1456	vitro	1452:1456	vitro	1452:1456	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	2	58	theme	conventional	402:413	arg1	model					433:437	a conventional gastric monolayer model	400:437	a conventional gastric monolayer model	400:437	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	3	59	theme	alginate	582:589	arg1	hydrogel					591:598	a 3D RGD-modified alginate hydrogel	564:598	a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert	564:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	5	60	theme	therapeutics	1366:1377	arg1	tool					1347:1350	a reliable evaluation tool	1325:1350	a reliable evaluation tool of innovative therapeutics	1325:1377	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	5	60	theme	therapeutics	1366:1377	arg1	diagnosis					1383:1391	diagnosis	1383:1391	diagnosis of gastric diseases	1383:1411	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	2	61	theme	rapid	315:319	arg1	model					358:362	a rapid, complex, and innovative 3D in vitro model	313:362	a rapid, complex, and innovative 3D in vitro model of the gastric mucosa	313:384	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	6	62	dep	STATEMENT	1414:1422	arg1	limited					1491:1497	limited	1491:1497	limited	1491:1497	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	0	63	dep	in	26:27	arg1	vitro					29:33	vitro	29:33	vitro	29:33	Bioengineering a novel 3D in vitro model of gastric mucosa for stomach permeability studies.					
30308252	7	64	from	hydrogel	1756:1763	arg1	system					1817:1822	a Transwell® system	1804:1822	a Transwell® system	1804:1822	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	4	65	theme	nanoparticles	1043:1055	arg1	studies					984:990	permeability studies	971:990	permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	971:1055	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	9	66	theme	3D	2105:2106	arg1	model					2108:2112	This cellularized 3D model	2087:2112	This cellularized 3D model	2087:2112	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	1	67	dep	in	192:193	arg1	vitro					195:199	vitro	195:199	vitro	195:199	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	6	68	theme	gastric	1472:1478	arg1	mucosa					1480:1485	the gastric mucosa	1468:1485	the gastric mucosa	1468:1485	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	1	69	theme	models	201:206	arg1	lack					175:178	the lack	171:178	the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined	171:294	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	4	70	theme	gastric	1060:1066	arg1	conditions					1068:1077	gastric conditions	1060:1077	gastric conditions	1060:1077	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	5	71	theme	evaluation	1336:1345	arg1	tool					1347:1350	a reliable evaluation tool	1325:1350	a reliable evaluation tool of innovative therapeutics	1325:1377	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	3	72	theme	insert	649:654	arg1	side					628:631	the basolateral side	612:631	the basolateral side of a Transwell® insert	612:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	2	73	theme	complex	322:328	arg1	model					358:362	a rapid, complex, and innovative 3D in vitro model	313:362	a rapid, complex, and innovative 3D in vitro model of the gastric mucosa	313:384	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	4	74	theme	fluorescent	1019:1029	arg1	nanoparticles					1043:1055	FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	995:1055	FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	995:1055	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	7	75	theme	epithelial	1769:1778	arg1	cells					1795:1799	epithelial gastric cancer cells	1769:1799	epithelial gastric cancer cells	1769:1799	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	6	76	theme	Current	1441:1447	arg1	models					1458:1463	Current in vitro models	1441:1463	Current in vitro models of the gastric mucosa	1441:1485	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	5	77	theme	gastric	1196:1202	arg1	ECM					1226:1228	ECM	1226:1228	ECM	1226:1228	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	5	77	theme	gastric	1196:1202	arg1	matrix					1218:1223	a gastric extracellular matrix	1194:1223	a gastric extracellular matrix (ECM)	1194:1229	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	9	78	dep	treatment	2208:2216	arg1	the					2204:2206	the	2204:2206	the	2204:2206	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	7	79	theme	gastric	1780:1786	arg1	cells					1795:1799	epithelial gastric cancer cells	1769:1799	epithelial gastric cancer cells	1769:1799	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	3	80	from	hydrogel	591:598	arg1	embedded					552:559	embedded	552:559	embedded	552:559	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	3	81	theme	extracellular	671:683	arg1	matrix					685:690	the extracellular matrix	667:690	the extracellular matrix	667:690	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	6	82	theme	culture	1521:1527	arg1	systems					1529:1535	simplistic 2D cell culture systems	1502:1535	simplistic 2D cell culture systems	1502:1535	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	8	83	theme	Epithelial	1886:1895	arg1	cells					1897:1901	Epithelial cells	1886:1901	Epithelial cells	1886:1901	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	5	84	theme	gastric	1396:1402	arg1	diseases					1404:1411	gastric diseases	1396:1411	gastric diseases	1396:1411	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	3	85	theme	developed	499:507	arg1	model					509:513	The developed model	495:513	The developed model	495:513	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	6	86	theme	2D	1513:1514	arg1	systems					1529:1535	simplistic 2D cell culture systems	1502:1535	simplistic 2D cell culture systems	1502:1535	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	7	87	theme	alginate	1747:1754	arg1	hydrogel					1756:1763	a 3D RGD-modified alginate hydrogel	1729:1763	a 3D RGD-modified alginate hydrogel	1729:1763	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	9	88	theme	cellularized	2092:2103	arg1	model					2108:2112	This cellularized 3D model	2087:2112	This cellularized 3D model	2087:2112	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	8	89	theme	cell-laden	1946:1955	arg1	hydrogel					1960:1967	the cell-laden 3D hydrogel	1942:1967	the cell-laden 3D hydrogel	1942:1967	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	2	90	theme	lamina	479:484	arg1	propria					486:492	the lamina propria	475:492	the lamina propria	475:492	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	9	91	theme	strategies	2189:2198	arg1	development					2170:2180	the development	2166:2180	the development of new strategies for the treatment and diagnosis of gastric diseases	2166:2250	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	8	92	theme	gastric	1916:1922	arg1	epithelium					1924:1933	the gastric epithelium	1912:1933	the gastric epithelium	1912:1933	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	3	93	theme	stomach	532:538	arg1	fibroblasts					540:550	normal stomach fibroblasts	525:550	normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert	525:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	1	94	theme	in	192:193	arg1	models					201:206	reliable in vitro models	183:206	reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined	183:294	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	3	95	theme	propria	729:735	arg1	matrix					685:690	the extracellular matrix	667:690	the extracellular matrix	667:690	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	3	95	theme	propria	729:735	arg1	component					705:713	cellular component	696:713	cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier	696:892	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	4	96	dep	in	934:935	arg1	vitro					937:941	vitro	937:941	vitro	937:941	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	1	97	theme	stomach-directed	106:121	arg1	therapeutics					123:134	stomach-directed therapeutics	106:134	stomach-directed therapeutics	106:134	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	7	98	theme	RGD-modified	1734:1745	arg1	hydrogel					1756:1763	a 3D RGD-modified alginate hydrogel	1729:1763	a 3D RGD-modified alginate hydrogel	1729:1763	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	6	99	theme	therapeutic	1633:1643	arg1	approaches					1645:1654	new therapeutic approaches	1629:1654	new therapeutic approaches to gastric pathologies	1629:1677	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	0	100	theme	mucosa	52:57	arg1	model					35:39	a novel 3D in vitro model	15:39	a novel 3D in vitro model of gastric mucosa for stomach permeability studies	15:90	Bioengineering a novel 3D in vitro model of gastric mucosa for stomach permeability studies.					
30308252	3	101	theme	embedded	552:559	arg1	fibroblasts					540:550	normal stomach fibroblasts	525:550	normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert	525:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	5	102	theme	-like	1230:1234	arg1	microenvironment					1236:1251	a gastric extracellular matrix (ECM)-like microenvironment	1194:1251	a gastric extracellular matrix (ECM)-like microenvironment	1194:1251	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	9	103	theme	diseases	2243:2250	arg1	diagnosis					2222:2230	diagnosis	2222:2230	diagnosis	2222:2230	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	9	103	theme	diseases	2243:2250	arg1	treatment					2208:2216	treatment	2208:2216	treatment	2208:2216	This cellularized 3D model stands as a promising evaluation platform to assist the development of new strategies for the treatment and diagnosis of gastric diseases.					
30308252	0	104	theme	stomach	63:69	arg1	studies					84:90	stomach permeability studies	63:90	stomach permeability studies	63:90	Bioengineering a novel 3D in vitro model of gastric mucosa for stomach permeability studies.					
30308252	7	105	theme	embedded	1713:1720	arg1	fibroblasts					1701:1711	stomach fibroblasts	1693:1711	stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system	1693:1822	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	3	106	theme	stomach	792:798	arg1	MKN74					811:815	MKN74	811:815	MKN74	811:815	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	3	106	theme	stomach	792:798	arg1	line					805:808	a moderately differentiated adenocarcinoma stomach cell line	749:808	a moderately differentiated adenocarcinoma stomach cell line (MKN74)	749:816	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	7	107	theme	stomach	1693:1699	arg1	fibroblasts					1701:1711	stomach fibroblasts	1693:1711	stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system	1693:1822	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	3	108	from	embedded	552:559	arg1	hydrogel					591:598	a 3D RGD-modified alginate hydrogel	564:598	a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert	564:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	3	109	theme	differentiated	762:775	arg1	MKN74					811:815	MKN74	811:815	MKN74	811:815	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	3	109	theme	differentiated	762:775	arg1	line					805:808	a moderately differentiated adenocarcinoma stomach cell line	749:808	a moderately differentiated adenocarcinoma stomach cell line (MKN74)	749:816	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	8	110	theme	propria	2078:2084	arg1	matrix					2053:2058	the extracellular matrix	2035:2058	the extracellular matrix of gastric lamina propria	2035:2084	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	2	111	theme	in	349:350	arg1	model					358:362	a rapid, complex, and innovative 3D in vitro model	313:362	a rapid, complex, and innovative 3D in vitro model of the gastric mucosa	313:384	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	2	112	theme	innovative	335:344	arg1	model					358:362	a rapid, complex, and innovative 3D in vitro model	313:362	a rapid, complex, and innovative 3D in vitro model of the gastric mucosa	313:384	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	0	113	theme	3D	23:24	arg1	model					35:39	a novel 3D in vitro model	15:39	a novel 3D in vitro model of gastric mucosa for stomach permeability studies	15:90	Bioengineering a novel 3D in vitro model of gastric mucosa for stomach permeability studies.					
30308252	1	114	theme	cancer	260:265	arg1	cells					267:271	gastric cancer cells	252:271	gastric cancer cells	252:271	The field of stomach-directed therapeutics and diagnosis is still hampered by the lack of reliable in vitro models that closely mimic the gastric mucosa where gastric cancer cells are generally confined.					
30308252	6	115	theme	wall	1585:1588	arg1	dimensionality					1555:1568	the dimensionality	1551:1568	the dimensionality of the stomach wall	1551:1588	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	3	116	theme	barrier	886:892	arg1	conditions					860:869	the physiological conditions	842:869	the physiological conditions of the gastric barrier	842:892	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	2	117	theme	mucosa	379:384	arg1	model					358:362	a rapid, complex, and innovative 3D in vitro model	313:362	a rapid, complex, and innovative 3D in vitro model of the gastric mucosa	313:384	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	8	118	theme	matrix	2053:2058	arg1	dimensionality					2017:2030	dimensionality	2017:2030	dimensionality	2017:2030	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	8	118	theme	matrix	2053:2058	arg1	composition					2001:2011	cellular composition	1992:2011	cellular composition	1992:2011	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	7	119	theme	stomach	1870:1876	arg1	mucosa					1878:1883	the stomach mucosa	1866:1883	the stomach mucosa	1866:1883	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	2	120	theme	gastric	415:421	arg1	model					433:437	a conventional gastric monolayer model	400:437	a conventional gastric monolayer model	400:437	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30308252	3	121	theme	RGD-modified	569:580	arg1	hydrogel					591:598	a 3D RGD-modified alginate hydrogel	564:598	a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert	564:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	5	122	theme	innovative	1355:1364	arg1	therapeutics					1366:1377	innovative therapeutics	1355:1377	innovative therapeutics	1355:1377	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	8	123	theme	gastric	2063:2069	arg1	propria					2078:2084	gastric lamina propria	2063:2084	gastric lamina propria	2063:2084	Epithelial cells recreate the gastric epithelium, while the cell-laden 3D hydrogel recapitulates both the cellular composition and dimensionality of the extracellular matrix of gastric lamina propria.					
30308252	7	124	theme	new	1842:1844	arg1	model					1857:1861	a new biomimetic model	1840:1861	a new biomimetic model of the stomach mucosa	1840:1883	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	3	125	theme	basolateral	616:626	arg1	side					628:631	the basolateral side	612:631	the basolateral side of a Transwell® insert	612:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	6	126	theme	mucosa	1480:1485	arg1	models					1458:1463	Current in vitro models	1441:1463	Current in vitro models of the gastric mucosa	1441:1485	STATEMENT OF SIGNIFICANCE: Current in vitro models of the gastric mucosa are limited to simplistic 2D cell culture systems, which ignore the dimensionality of the stomach wall and make it difficult to reliably test new therapeutic approaches to gastric pathologies.					
30308252	5	127	theme	biomimetic	1149:1158	arg1	propria					1167:1173	the biomimetic lamina propria	1145:1173	the biomimetic lamina propria	1145:1173	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	7	128	theme	biomimetic	1846:1855	arg1	model					1857:1861	a new biomimetic model	1840:1861	a new biomimetic model of the stomach mucosa	1840:1883	By combining stomach fibroblasts embedded within a 3D RGD-modified alginate hydrogel and epithelial gastric cancer cells in a Transwell® system, we established a new biomimetic model of the stomach mucosa.					
30308252	4	129	theme	polystyrene	1031:1041	arg1	nanoparticles					1043:1055	FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	995:1055	FITC-dextran and 200 nm fluorescent polystyrene nanoparticles	995:1055	The integrity and functionality of the in vitro model was evaluated through permeability studies of FITC-dextran and 200 nm fluorescent polystyrene nanoparticles at gastric conditions.					
30308252	5	130	theme	reliable	1327:1334	arg1	tool					1347:1350	a reliable evaluation tool	1325:1350	a reliable evaluation tool of innovative therapeutics	1325:1377	Nanoparticle transport was pH-dependent and strongly impacted by the biomimetic lamina propria, highlighting that a gastric extracellular matrix (ECM)-like microenvironment should be integrated in an in vitro permeability model to be adopted as a reliable evaluation tool of innovative therapeutics and diagnosis of gastric diseases.					
30308252	3	131	theme	Transwell®	638:647	arg1	insert					649:654	a Transwell® insert	636:654	a Transwell® insert	636:654	The developed model comprises normal stomach fibroblasts embedded in a 3D RGD-modified alginate hydrogel prepared on the basolateral side of a Transwell® insert, mimicking the extracellular matrix and cellular component of the lamina propria, onto which a moderately differentiated adenocarcinoma stomach cell line (MKN74) was seeded, reproducing the physiological conditions of the gastric barrier.					
30308252	2	132	dep	in	349:350	arg1	vitro					352:356	vitro	352:356	vitro	352:356	Here we propose a rapid, complex, and innovative 3D in vitro model of the gastric mucosa, by extending a conventional gastric monolayer model to an inner stratum of the mucosa - the lamina propria.					
30940592	1	0	with	TiO2	126:129	arg1	chitosan					184:191	chitosan	184:191	chitosan (NST/CS, NSZ/CS)	184:208	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	8	1	theme	as-synthesized	1079:1092	arg1	NST/CS					1094:1099	as-synthesized NST/CS	1079:1099	as-synthesized NST/CS	1079:1099	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	4	2	theme	tetracycline	497:508	arg1	efficiency					522:531	the highest tetracycline degradation efficiency	485:531	the highest tetracycline degradation efficiency of 91% for duration of 20 min	485:561	At screening test, NST/CS had the highest tetracycline degradation efficiency of 91% for duration of 20 min under visible light.					
30940592	8	3	from	water	1232:1236	arg1	degradation					1195:1205	degradation	1195:1205	degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants	1195:1332	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	2	4	theme	prepared	263:270	arg1	samples					272:278	The prepared samples	259:278	The prepared samples	259:278	The prepared samples were characterized using XRD, FTIR, TEM and BET techniques.					
30940592	4	5	theme	highest	489:495	arg1	efficiency					522:531	the highest tetracycline degradation efficiency	485:531	the highest tetracycline degradation efficiency of 91% for duration of 20 min	485:561	At screening test, NST/CS had the highest tetracycline degradation efficiency of 91% for duration of 20 min under visible light.					
30940592	0	6	theme	light	65:69	arg1	degradation					86:96	visible light photocatalytic degradation	57:96	visible light photocatalytic degradation of tetracycline	57:112	Chitosan modified N, S-doped TiO2 and N, S-doped ZnO for visible light photocatalytic degradation of tetracycline.					
30940592	6	7	theme	detail	700:705	arg1	characterization					707:722	A detail characterization	698:722	A detail characterization including HRTEM, SEM, EDS and DRS	698:756	A detail characterization including HRTEM, SEM, EDS and DRS were conducted for NST/CS, the most active photocatalyst in this study.					
30940592	9	8	theme	tetracycline	1498:1509	arg1	removal					1511:1517	tetracycline removal	1498:1517	tetracycline removal in water resource	1498:1535	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	4	9	contain	had	481:483	arg1	NST/CS					474:479	NST/CS	474:479	NST/CS	474:479	At screening test, NST/CS had the highest tetracycline degradation efficiency of 91% for duration of 20 min under visible light.					
30940592	4	9	contain	had	481:483	arg2	efficiency					522:531	the highest tetracycline degradation efficiency	485:531	the highest tetracycline degradation efficiency of 91% for duration of 20 min	485:561	At screening test, NST/CS had the highest tetracycline degradation efficiency of 91% for duration of 20 min under visible light.					
30940592	0	10	theme	visible	57:63	arg1	light					65:69	visible light	57:69	visible light photocatalytic degradation of tetracycline	57:112	Chitosan modified N, S-doped TiO2 and N, S-doped ZnO for visible light photocatalytic degradation of tetracycline.					
30940592	6	11	theme	active	794:799	arg1	photocatalyst					801:813	the most active photocatalyst	785:813	the most active photocatalyst in this study	785:827	A detail characterization including HRTEM, SEM, EDS and DRS were conducted for NST/CS, the most active photocatalyst in this study.					
30940592	6	11	theme	active	794:799	arg1	NST/CS					777:782	NST/CS	777:782	NST/CS	777:782	A detail characterization including HRTEM, SEM, EDS and DRS were conducted for NST/CS, the most active photocatalyst in this study.					
30940592	8	12	from	substances	1218:1227	arg1	wastewater					1242:1251	wastewater	1242:1251	wastewater especially for degradation of emerging pollutants	1242:1301	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	8	12	from	substances	1218:1227	arg1	water					1232:1236	water	1232:1236	water	1232:1236	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	9	13	theme	pollutants	1483:1492	arg1	treatment					1455:1463	the treatment	1451:1463	the treatment of pharmaceutical pollutants	1451:1492	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	4	14	theme	degradation	510:520	arg1	efficiency					522:531	the highest tetracycline degradation efficiency	485:531	the highest tetracycline degradation efficiency of 91% for duration of 20 min	485:561	At screening test, NST/CS had the highest tetracycline degradation efficiency of 91% for duration of 20 min under visible light.					
30940592	5	15	theme	degradation	655:665	arg1	rate					632:635	the rate	628:635	the rate of photocatalytic degradation of tetracycline	628:681	The blending of chitosan with NST increases the rate of photocatalytic degradation of tetracycline about 2 times.					
30940592	9	16	theme	pharmaceutical	1468:1481	arg1	pollutants					1483:1492	pharmaceutical pollutants	1468:1492	pharmaceutical pollutants	1468:1492	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	9	17	theme	pharmaceutical	1414:1427	arg1	facilities					1429:1438	pharmaceutical facilities	1414:1438	pharmaceutical facilities	1414:1438	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	8	18	theme	pollutants	1292:1301	arg1	degradation					1268:1278	degradation	1268:1278	degradation of emerging pollutants	1268:1301	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	5	19	with	blending	588:595	arg1	NST					614:616	NST	614:616	NST	614:616	The blending of chitosan with NST increases the rate of photocatalytic degradation of tetracycline about 2 times.					
30940592	2	20	theme	FTIR	310:313	arg1	techniques					328:337	XRD, FTIR, TEM and BET techniques	305:337	XRD, FTIR, TEM and BET techniques	305:337	The prepared samples were characterized using XRD, FTIR, TEM and BET techniques.					
30940592	0	21	theme	photocatalytic	71:84	arg1	degradation					86:96	visible light photocatalytic degradation	57:96	visible light photocatalytic degradation of tetracycline	57:112	Chitosan modified N, S-doped TiO2 and N, S-doped ZnO for visible light photocatalytic degradation of tetracycline.					
30940592	1	22	with	N	115:115	arg1	chitosan					184:191	chitosan	184:191	chitosan (NST/CS, NSZ/CS)	184:208	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	7	23	theme	activity	845:852	arg1	test					854:857	Photocatalytic activity test	830:857	Photocatalytic activity test	830:857	Photocatalytic activity test was conducted by varying tetracycline concentration, irradiation time, catalyst's concentration and pH using response surface methodology to find out the optimum condition for photocatalytic activity.					
30940592	8	24	theme	emerging	1283:1290	arg1	pollutants					1292:1301	emerging pollutants	1283:1301	emerging pollutants	1283:1301	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	4	25	theme	visible	569:575	arg1	light					577:581	visible light	569:581	visible light	569:581	At screening test, NST/CS had the highest tetracycline degradation efficiency of 91% for duration of 20 min under visible light.					
30940592	5	26	theme	tetracycline	670:681	arg1	degradation					655:665	photocatalytic degradation	640:665	photocatalytic degradation of tetracycline	640:681	The blending of chitosan with NST increases the rate of photocatalytic degradation of tetracycline about 2 times.					
30940592	3	27	used	used	366:369	arg2	photocatalysts					346:359	These photocatalysts	340:359	These photocatalysts	340:359	These photocatalysts were used for the photocatalytic degradation of tetracycline under visible light irradiation.					
30940592	1	28	with	ZnO	149:151	arg1	chitosan					184:191	chitosan	184:191	chitosan (NST/CS, NSZ/CS)	184:208	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	2	29	theme	XRD	305:307	arg1	techniques					328:337	XRD, FTIR, TEM and BET techniques	305:337	XRD, FTIR, TEM and BET techniques	305:337	The prepared samples were characterized using XRD, FTIR, TEM and BET techniques.					
30940592	9	30	from	work	1357:1360	arg1	results					1339:1345	The results	1335:1345	The results from this work	1335:1360	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	8	31	from	wastewater	1242:1251	arg1	degradation					1195:1205	degradation	1195:1205	degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants	1195:1332	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	8	32	theme	such	1303:1306	arg1	pollutants					1323:1332	such pharmaceutical pollutants	1303:1332	such pharmaceutical pollutants	1303:1332	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	8	33	from	degradation	1195:1205	arg1	wastewater					1242:1251	wastewater	1242:1251	wastewater especially for degradation of emerging pollutants	1242:1301	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	8	33	from	degradation	1195:1205	arg1	water					1232:1236	water	1232:1236	water	1232:1236	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	9	34	from	use	1443:1445	arg1	resource					1528:1535	water resource	1522:1535	water resource	1522:1535	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	0	35	theme	tetracycline	101:112	arg1	degradation					86:96	visible light photocatalytic degradation	57:96	visible light photocatalytic degradation of tetracycline	57:112	Chitosan modified N, S-doped TiO2 and N, S-doped ZnO for visible light photocatalytic degradation of tetracycline.					
30940592	7	36	theme	Photocatalytic	830:843	arg1	test					854:857	Photocatalytic activity test	830:857	Photocatalytic activity test	830:857	Photocatalytic activity test was conducted by varying tetracycline concentration, irradiation time, catalyst's concentration and pH using response surface methodology to find out the optimum condition for photocatalytic activity.					
30940592	9	37	from	removal	1511:1517	arg1	resource					1528:1535	water resource	1522:1535	water resource	1522:1535	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	2	38	theme	BET	324:326	arg1	techniques					328:337	XRD, FTIR, TEM and BET techniques	305:337	XRD, FTIR, TEM and BET techniques	305:337	The prepared samples were characterized using XRD, FTIR, TEM and BET techniques.					
30940592	8	39	theme	pharmaceutical	1308:1321	arg1	pollutants					1323:1332	such pharmaceutical pollutants	1303:1332	such pharmaceutical pollutants	1303:1332	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	3	40	theme	visible	428:434	arg1	irradiation					442:452	visible light irradiation	428:452	visible light irradiation	428:452	These photocatalysts were used for the photocatalytic degradation of tetracycline under visible light irradiation.					
30940592	9	41	theme	promising	1369:1377	arg1	material					1379:1386	a promising material	1367:1386	a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource	1367:1535	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	1	42	theme	S-doped	141:147	arg1	NSZ					154:156	NSZ	154:156	NSZ	154:156	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	1	42	theme	S-doped	141:147	arg1	ZnO					149:151	S-doped ZnO	141:151	S-doped ZnO (NSZ)	141:157	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	8	43	theme	organic	1210:1216	arg1	substances					1218:1227	organic substances	1210:1227	organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants	1210:1332	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	7	44	theme	response	968:975	arg1	methodology					985:995	response surface methodology	968:995	response surface methodology	968:995	Photocatalytic activity test was conducted by varying tetracycline concentration, irradiation time, catalyst's concentration and pH using response surface methodology to find out the optimum condition for photocatalytic activity.					
30940592	2	45	theme	TEM	316:318	arg1	techniques					328:337	XRD, FTIR, TEM and BET techniques	305:337	XRD, FTIR, TEM and BET techniques	305:337	The prepared samples were characterized using XRD, FTIR, TEM and BET techniques.					
30940592	0	46	theme	S-doped	21:27	arg1	TiO2					29:32	S-doped TiO2	21:32	S-doped TiO2	21:32	Chitosan modified N, S-doped TiO2 and N, S-doped ZnO for visible light photocatalytic degradation of tetracycline.					
30940592	7	47	theme	photocatalytic	1035:1048	arg1	activity					1050:1057	photocatalytic activity	1035:1057	photocatalytic activity	1035:1057	Photocatalytic activity test was conducted by varying tetracycline concentration, irradiation time, catalyst's concentration and pH using response surface methodology to find out the optimum condition for photocatalytic activity.					
30940592	7	48	theme	surface	977:983	arg1	methodology					985:995	response surface methodology	968:995	response surface methodology	968:995	Photocatalytic activity test was conducted by varying tetracycline concentration, irradiation time, catalyst's concentration and pH using response surface methodology to find out the optimum condition for photocatalytic activity.					
30940592	5	49	theme	photocatalytic	640:653	arg1	degradation					655:665	photocatalytic degradation	640:665	photocatalytic degradation of tetracycline	640:681	The blending of chitosan with NST increases the rate of photocatalytic degradation of tetracycline about 2 times.					
30940592	4	50	theme	screening	458:466	arg1	test					468:471	screening test	458:471	screening test	458:471	At screening test, NST/CS had the highest tetracycline degradation efficiency of 91% for duration of 20 min under visible light.					
30940592	8	51	theme	NST/CS	1094:1099	arg1	reusability					1064:1074	The reusability	1060:1074	The reusability of as-synthesized NST/CS	1060:1099	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	8	52	theme	high	1131:1134	arg1	recoverability					1136:1149	its high recoverability	1127:1149	its high recoverability	1127:1149	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	8	53	theme	substances	1218:1227	arg1	degradation					1195:1205	degradation	1195:1205	degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants	1195:1332	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	1	54	with	N	138:138	arg1	chitosan					184:191	chitosan	184:191	chitosan (NST/CS, NSZ/CS)	184:208	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	1	55	dep	chitosan	184:191	arg1	NSZ/CS					202:207	NSZ/CS	202:207	NSZ/CS	202:207	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	1	55	dep	chitosan	184:191	arg1	NST/CS					194:199	NST/CS	194:199	NST/CS	194:199	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	7	56	theme	optimum	1013:1019	arg1	condition					1021:1029	the optimum condition	1009:1029	the optimum condition for photocatalytic activity	1009:1057	Photocatalytic activity test was conducted by varying tetracycline concentration, irradiation time, catalyst's concentration and pH using response surface methodology to find out the optimum condition for photocatalytic activity.					
30940592	1	57	with	composite	169:177	arg1	chitosan					184:191	chitosan	184:191	chitosan (NST/CS, NSZ/CS)	184:208	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	3	58	theme	tetracycline	409:420	arg1	degradation					394:404	the photocatalytic degradation	375:404	the photocatalytic degradation of tetracycline under visible light irradiation	375:452	These photocatalysts were used for the photocatalytic degradation of tetracycline under visible light irradiation.					
30940592	1	59	theme	sol	230:232	arg1	method					251:256	sol gel-hydrothermal method	230:256	sol gel-hydrothermal method	230:256	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	5	60	theme	chitosan	600:607	arg1	blending					588:595	The blending	584:595	The blending of chitosan with NST	584:616	The blending of chitosan with NST increases the rate of photocatalytic degradation of tetracycline about 2 times.					
30940592	8	61	dep	water	1232:1236	arg1	pollutants					1323:1332	such pharmaceutical pollutants	1303:1332	such pharmaceutical pollutants	1303:1332	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	8	62	theme	effective	1172:1180	arg1	which					1114:1118	which	1114:1118	which	1114:1118	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	8	62	theme	effective	1172:1180	arg1	catalyst					1182:1189	an effective catalyst	1169:1189	an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants	1169:1332	The reusability of as-synthesized NST/CS was assessed which due to its high recoverability can be applied as an effective catalyst for degradation of organic substances in water and wastewater especially for degradation of emerging pollutants such pharmaceutical pollutants.					
30940592	3	63	theme	photocatalytic	379:392	arg1	degradation					394:404	the photocatalytic degradation	375:404	the photocatalytic degradation of tetracycline under visible light irradiation	375:452	These photocatalysts were used for the photocatalytic degradation of tetracycline under visible light irradiation.					
30940592	9	64	theme	water	1522:1526	arg1	resource					1528:1535	water resource	1522:1535	water resource	1522:1535	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	9	65	theme	local	1392:1396	arg1	authorities					1398:1408	local authorities	1392:1408	local authorities	1392:1408	The results from this work show a promising material for local authorities and pharmaceutical facilities to use for the treatment of pharmaceutical pollutants and tetracycline removal in water resource.					
30940592	0	66	theme	S-doped	41:47	arg1	ZnO					49:51	S-doped ZnO	41:51	S-doped ZnO for visible light photocatalytic degradation of tetracycline	41:112	Chitosan modified N, S-doped TiO2 and N, S-doped ZnO for visible light photocatalytic degradation of tetracycline.					
30940592	0	66	theme	S-doped	41:47	arg1	N					38:38	N	38:38	N	38:38	Chitosan modified N, S-doped TiO2 and N, S-doped ZnO for visible light photocatalytic degradation of tetracycline.					
30940592	4	67	theme	20 min	556:561	arg1	duration					544:551	duration	544:551	duration of 20 min	544:561	At screening test, NST/CS had the highest tetracycline degradation efficiency of 91% for duration of 20 min under visible light.					
30940592	7	68	theme	irradiation	912:922	arg1	time					924:927	irradiation time	912:927	irradiation time	912:927	Photocatalytic activity test was conducted by varying tetracycline concentration, irradiation time, catalyst's concentration and pH using response surface methodology to find out the optimum condition for photocatalytic activity.					
30940592	3	69	theme	light	436:440	arg1	irradiation					442:452	visible light irradiation	428:452	visible light irradiation	428:452	These photocatalysts were used for the photocatalytic degradation of tetracycline under visible light irradiation.					
30940592	6	70	from	photocatalyst	801:813	arg1	study					823:827	this study	818:827	this study	818:827	A detail characterization including HRTEM, SEM, EDS and DRS were conducted for NST/CS, the most active photocatalyst in this study.					
30940592	1	71	theme	gel-hydrothermal	234:249	arg1	method					251:256	sol gel-hydrothermal method	230:256	sol gel-hydrothermal method	230:256	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	7	72	theme	tetracycline	884:895	arg1	concentration					897:909	tetracycline concentration	884:909	tetracycline concentration	884:909	Photocatalytic activity test was conducted by varying tetracycline concentration, irradiation time, catalyst's concentration and pH using response surface methodology to find out the optimum condition for photocatalytic activity.					
30940592	1	73	theme	S-doped	118:124	arg1	NST					132:134	NST	132:134	NST	132:134	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	1	73	theme	S-doped	118:124	arg1	TiO2					126:129	S-doped TiO2	118:129	S-doped TiO2 (NST)	118:135	N, S-doped TiO2 (NST), N, S-doped ZnO (NSZ) and their composite with chitosan (NST/CS, NSZ/CS) were synthesized by sol gel-hydrothermal method.					
30940592	4	74	theme	%	538:538	arg1	efficiency					522:531	the highest tetracycline degradation efficiency	485:531	the highest tetracycline degradation efficiency of 91% for duration of 20 min	485:561	At screening test, NST/CS had the highest tetracycline degradation efficiency of 91% for duration of 20 min under visible light.					
31252145	5	0	theme	square	803:808	arg1	error					810:814	root mean square error	793:814	root mean square error	793:814	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	4	1	theme	different	676:684	arg1	measurements					700:711	the different spectroscopic measurements	672:711	the different spectroscopic measurements	672:711	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	1	2	theme	Analytical	267:276	arg1	Technology					278:287	Process Analytical Technology	259:287	Process Analytical Technology (PAT) data	259:298	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	1	2	theme	Analytical	267:276	arg1	PAT					290:292	PAT	290:292	PAT	290:292	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	7	3	theme	data	1196:1199	arg1	method					1208:1213	a straightforward data fusion method	1178:1213	a straightforward data fusion method without the need for additional preprocessing steps	1178:1265	Furthermore, ANN served as a straightforward data fusion method without the need for additional preprocessing steps.					
31252145	7	3	theme	data	1196:1199	arg1	ANN					1164:1166	ANN	1164:1166	ANN	1164:1166	Furthermore, ANN served as a straightforward data fusion method without the need for additional preprocessing steps.					
31252145	1	4	theme	Technology	278:287	arg1	data					295:298	Process Analytical Technology (PAT) data	259:298	Process Analytical Technology (PAT) data	259:298	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	4	5	theme	Least	608:612	arg1	PLS					622:624	PLS	622:624	PLS	622:624	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	4	5	theme	Least	608:612	arg1	Square					614:619	Partial Least Square	600:619	Partial Least Square (PLS)	600:625	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	8	6	theme	data	1307:1310	arg1	processing					1312:1321	data processing	1307:1321	data processing	1307:1321	The method could significantly advance data processing in the PAT environment, contribute to an enhanced real-time release testing procedure and hence the increased efficacy of dissolution testing.					
31252145	4	7	dep	different	676:684	arg1	spectroscopic					686:698	spectroscopic	686:698	spectroscopic	686:698	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	8	8	theme	PAT	1330:1332	arg1	environment					1334:1344	the PAT environment	1326:1344	the PAT environment	1326:1344	The method could significantly advance data processing in the PAT environment, contribute to an enhanced real-time release testing procedure and hence the increased efficacy of dissolution testing.					
31252145	5	9	dep	lower	787:791	arg1	error					810:814	root mean square error	793:814	root mean square error	793:814	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	5	10	theme	process	920:926	arg1	parameters					928:937	the process parameters	916:937	the process parameters	916:937	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	6	11	theme	f1	1066:1067	arg1	values					1076:1081	6.5 and 63 mean f1 and f2 values	1050:1081	6.5 and 63 mean f1 and f2 values	1050:1081	The ANN model using reflection NIR spectra provided the most accurate predictions with 6.5 and 63 mean f1 and f2 values between the computed and measured dissolution curves, respectively.					
31252145	6	12	theme	dissolution	1117:1127	arg1	curves					1129:1134	measured dissolution curves	1108:1134	measured dissolution curves	1108:1134	The ANN model using reflection NIR spectra provided the most accurate predictions with 6.5 and 63 mean f1 and f2 values between the computed and measured dissolution curves, respectively.					
31252145	4	13	theme	ANN	642:644	arg1	models					646:651	Partial Least Square (PLS) regression and ANN models	600:651	models	646:651	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	8	14	theme	dissolution	1445:1455	arg1	testing					1457:1463	dissolution testing	1445:1463	dissolution testing	1445:1463	The method could significantly advance data processing in the PAT environment, contribute to an enhanced real-time release testing procedure and hence the increased efficacy of dissolution testing.					
31252145	5	15	dep	3	784:784	arg1	to					781:782	to	781:782	to	781:782	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	4	16	dep	developed	658:666	arg1	combining					740:748	combining	740:748	combining them together	740:762	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	4	16	dep	developed	658:666	arg1	individually					713:724	individually	713:724	individually	713:724	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	8	17	theme	real-time	1373:1381	arg1	release					1383:1389	an enhanced real-time release	1361:1389	an enhanced real-time release testing procedure	1361:1407	The method could significantly advance data processing in the PAT environment, contribute to an enhanced real-time release testing procedure and hence the increased efficacy of dissolution testing.					
31252145	7	18	theme	additional	1236:1245	arg1	steps					1261:1265	additional preprocessing steps	1236:1265	additional preprocessing steps	1236:1265	Furthermore, ANN served as a straightforward data fusion method without the need for additional preprocessing steps.					
31252145	5	19	theme	non-linearity	894:906	arg1	capability					871:880	its capability	867:880	its capability of modeling non-linearity between the process parameters and dissolution curves	867:960	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	6	20	theme	accurate	1024:1031	arg1	predictions					1033:1043	the most accurate predictions	1015:1043	the most accurate predictions	1015:1043	The ANN model using reflection NIR spectra provided the most accurate predictions with 6.5 and 63 mean f1 and f2 values between the computed and measured dissolution curves, respectively.					
31252145	6	21	theme	reflection	983:992	arg1	spectra					998:1004	reflection NIR spectra	983:1004	reflection NIR spectra	983:1004	The ANN model using reflection NIR spectra provided the most accurate predictions with 6.5 and 63 mean f1 and f2 values between the computed and measured dissolution curves, respectively.					
31252145	1	22	from	data	295:298	arg1	dissolution					216:226	the in vitro dissolution	203:226	the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data	203:298	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	2	23	theme	tablet	321:326	arg1	formulation					328:338	An extended release tablet formulation	301:338	An extended release tablet formulation	301:338	An extended release tablet formulation was studied, where the dissolution was influenced by the composition of the tablets and the tableting compression force.					
31252145	0	24	theme	neural	26:31	arg1	networks					33:40	artificial neural networks	15:40	artificial neural networks	15:40	Application of artificial neural networks for Process Analytical Technology-based dissolution testing.					
31252145	2	25	theme	release	313:319	arg1	formulation					328:338	An extended release tablet formulation	301:338	An extended release tablet formulation	301:338	An extended release tablet formulation was studied, where the dissolution was influenced by the composition of the tablets and the tableting compression force.					
31252145	1	26	theme	in	207:208	arg1	dissolution					216:226	the in vitro dissolution	203:226	the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data	203:298	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	0	27	theme	artificial	15:24	arg1	networks					33:40	artificial neural networks	15:40	artificial neural networks	15:40	Application of artificial neural networks for Process Analytical Technology-based dissolution testing.					
31252145	5	28	theme	modeling	885:892	arg1	non-linearity					894:906	modeling non-linearity	885:906	modeling non-linearity between the process parameters and dissolution curves	885:960	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	6	29	theme	ANN	967:969	arg1	model					971:975	The ANN model	963:975	The ANN model using reflection NIR spectra	963:1004	The ANN model using reflection NIR spectra provided the most accurate predictions with 6.5 and 63 mean f1 and f2 values between the computed and measured dissolution curves, respectively.					
31252145	2	30	theme	extended	304:311	arg1	formulation					328:338	An extended release tablet formulation	301:338	An extended release tablet formulation	301:338	An extended release tablet formulation was studied, where the dissolution was influenced by the composition of the tablets and the tableting compression force.					
31252145	6	31	theme	mean	1061:1064	arg1	values					1076:1081	6.5 and 63 mean f1 and f2 values	1050:1081	6.5 and 63 mean f1 and f2 values	1050:1081	The ANN model using reflection NIR spectra provided the most accurate predictions with 6.5 and 63 mean f1 and f2 values between the computed and measured dissolution curves, respectively.					
31252145	7	32	theme	preprocessing	1247:1259	arg1	steps					1261:1265	additional preprocessing steps	1236:1265	additional preprocessing steps	1236:1265	Furthermore, ANN served as a straightforward data fusion method without the need for additional preprocessing steps.					
31252145	6	33	theme	f2	1073:1074	arg1	values					1076:1081	6.5 and 63 mean f1 and f2 values	1050:1081	6.5 and 63 mean f1 and f2 values	1050:1081	The ANN model using reflection NIR spectra provided the most accurate predictions with 6.5 and 63 mean f1 and f2 values between the computed and measured dissolution curves, respectively.					
31252145	7	34	theme	fusion	1201:1206	arg1	method					1208:1213	a straightforward data fusion method	1178:1213	a straightforward data fusion method without the need for additional preprocessing steps	1178:1265	Furthermore, ANN served as a straightforward data fusion method without the need for additional preprocessing steps.					
31252145	7	34	theme	fusion	1201:1206	arg1	ANN					1164:1166	ANN	1164:1166	ANN	1164:1166	Furthermore, ANN served as a straightforward data fusion method without the need for additional preprocessing steps.					
31252145	3	35	theme	spectral	585:592	arg1	data					594:597	the spectral data	581:597	the spectral data	581:597	NIR and Raman spectra of the intact tablets were measured, and the dissolution of the tablets was modeled directly from the spectral data.					
31252145	2	36	theme	tablets	416:422	arg1	composition					397:407	the composition	393:407	the composition of the tablets	393:422	An extended release tablet formulation was studied, where the dissolution was influenced by the composition of the tablets and the tableting compression force.					
31252145	2	36	theme	tablets	416:422	arg1	force					454:458	the tableting compression force	428:458	the tableting compression force	428:458	An extended release tablet formulation was studied, where the dissolution was influenced by the composition of the tablets and the tableting compression force.					
31252145	0	37	theme	networks	33:40	arg1	Application					0:10	Application	0:10	Application of artificial neural networks for Process Analytical Technology-based dissolution testing.	0:101	Application of artificial neural networks for Process Analytical Technology-based dissolution testing.					
31252145	8	38	theme	increased	1423:1431	arg1	efficacy					1433:1440	the increased efficacy	1419:1440	the increased efficacy of dissolution testing	1419:1463	The method could significantly advance data processing in the PAT environment, contribute to an enhanced real-time release testing procedure and hence the increased efficacy of dissolution testing.					
31252145	2	39	theme	compression	442:452	arg1	force					454:458	the tableting compression force	428:458	the tableting compression force	428:458	An extended release tablet formulation was studied, where the dissolution was influenced by the composition of the tablets and the tableting compression force.					
31252145	5	40	theme	root	793:796	arg1	error					810:814	root mean square error	793:814	root mean square error	793:814	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	0	41	theme	Analytical	54:63	arg1	testing					94:100	Process Analytical Technology-based dissolution testing	46:100	Process Analytical Technology-based dissolution testing	46:100	Application of artificial neural networks for Process Analytical Technology-based dissolution testing.					
31252145	6	42	theme	NIR	994:996	arg1	spectra					998:1004	reflection NIR spectra	983:1004	reflection NIR spectra	983:1004	The ANN model using reflection NIR spectra provided the most accurate predictions with 6.5 and 63 mean f1 and f2 values between the computed and measured dissolution curves, respectively.					
31252145	8	43	theme	enhanced	1364:1371	arg1	release					1383:1389	an enhanced real-time release	1361:1389	an enhanced real-time release testing procedure	1361:1407	The method could significantly advance data processing in the PAT environment, contribute to an enhanced real-time release testing procedure and hence the increased efficacy of dissolution testing.					
31252145	3	44	theme	intact	490:495	arg1	tablets					497:503	the intact tablets	486:503	the intact tablets	486:503	NIR and Raman spectra of the intact tablets were measured, and the dissolution of the tablets was modeled directly from the spectral data.					
31252145	0	45	theme	Process	46:52	arg1	testing					94:100	Process Analytical Technology-based dissolution testing	46:100	Process Analytical Technology-based dissolution testing	46:100	Application of artificial neural networks for Process Analytical Technology-based dissolution testing.					
31252145	3	46	theme	tablets	497:503	arg1	NIR					461:463	NIR	461:463	NIR	461:463	NIR and Raman spectra of the intact tablets were measured, and the dissolution of the tablets was modeled directly from the spectral data.					
31252145	3	46	theme	tablets	497:503	arg1	spectra					475:481	Raman spectra	469:481	Raman spectra	469:481	NIR and Raman spectra of the intact tablets were measured, and the dissolution of the tablets was modeled directly from the spectral data.					
31252145	5	47	theme	dissolution	943:953	arg1	curves					955:960	dissolution curves	943:960	dissolution curves	943:960	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	1	48	theme	artificial	141:150	arg1	ANN					169:171	ANN	169:171	ANN	169:171	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	1	48	theme	artificial	141:150	arg1	networks					159:166	artificial neural networks	141:166	artificial neural networks (ANN)	141:172	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	3	49	theme	tablets	547:553	arg1	dissolution					528:538	the dissolution	524:538	the dissolution of the tablets	524:553	NIR and Raman spectra of the intact tablets were measured, and the dissolution of the tablets was modeled directly from the spectral data.					
31252145	1	50	theme	pharmaceutical	231:244	arg1	tablets					246:252	pharmaceutical tablets	231:252	pharmaceutical tablets	231:252	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	6	51	theme	measured	1108:1115	arg1	curves					1129:1134	measured dissolution curves	1108:1134	measured dissolution curves	1108:1134	The ANN model using reflection NIR spectra provided the most accurate predictions with 6.5 and 63 mean f1 and f2 values between the computed and measured dissolution curves, respectively.					
31252145	8	52	theme	testing	1457:1463	arg1	efficacy					1433:1440	the increased efficacy	1419:1440	the increased efficacy of dissolution testing	1419:1463	The method could significantly advance data processing in the PAT environment, contribute to an enhanced real-time release testing procedure and hence the increased efficacy of dissolution testing.					
31252145	5	53	theme	mean	798:801	arg1	error					810:814	root mean square error	793:814	root mean square error	793:814	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	1	54	theme	neural	152:157	arg1	ANN					169:171	ANN	169:171	ANN	169:171	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	1	54	theme	neural	152:157	arg1	networks					159:166	artificial neural networks	141:166	artificial neural networks (ANN)	141:172	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	1	55	theme	tablets	246:252	arg1	dissolution					216:226	the in vitro dissolution	203:226	the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data	203:298	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	0	56	theme	dissolution	82:92	arg1	testing					94:100	Process Analytical Technology-based dissolution testing	46:100	Process Analytical Technology-based dissolution testing	46:100	Application of artificial neural networks for Process Analytical Technology-based dissolution testing.					
31252145	5	57	theme	PLS	848:850	arg1	models					852:857	the PLS models	844:857	the PLS models	844:857	ANN provided up to 3% lower root mean square error for prediction (RMSEP) than the PLS models, due to its capability of modeling non-linearity between the process parameters and dissolution curves.					
31252145	1	58	dep	in	207:208	arg1	vitro					210:214	vitro	210:214	vitro	210:214	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	3	59	theme	Raman	469:473	arg1	spectra					475:481	Raman spectra	469:481	Raman spectra	469:481	NIR and Raman spectra of the intact tablets were measured, and the dissolution of the tablets was modeled directly from the spectral data.					
31252145	1	60	theme	networks	159:166	arg1	application					126:136	the application	122:136	the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data	122:298	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	4	61	theme	Square	614:619	arg1	regression					627:636	Partial Least Square (PLS) regression and ANN models	600:651	regression	627:636	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	0	62	theme	Technology-based	65:80	arg1	testing					94:100	Process Analytical Technology-based dissolution testing	46:100	Process Analytical Technology-based dissolution testing	46:100	Application of artificial neural networks for Process Analytical Technology-based dissolution testing.					
31252145	2	63	theme	tableting	432:440	arg1	force					454:458	the tableting compression force	428:458	the tableting compression force	428:458	An extended release tablet formulation was studied, where the dissolution was influenced by the composition of the tablets and the tableting compression force.					
31252145	4	64	theme	Partial	600:606	arg1	PLS					622:624	PLS	622:624	PLS	622:624	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	4	64	theme	Partial	600:606	arg1	Square					614:619	Partial Least Square	600:619	Partial Least Square (PLS)	600:625	Partial Least Square (PLS) regression and ANN models were developed for the different spectroscopic measurements individually as well as by combining them together.					
31252145	7	65	theme	straightforward	1180:1194	arg1	method					1208:1213	a straightforward data fusion method	1178:1213	a straightforward data fusion method without the need for additional preprocessing steps	1178:1265	Furthermore, ANN served as a straightforward data fusion method without the need for additional preprocessing steps.					
31252145	7	65	theme	straightforward	1180:1194	arg1	ANN					1164:1166	ANN	1164:1166	ANN	1164:1166	Furthermore, ANN served as a straightforward data fusion method without the need for additional preprocessing steps.					
31252145	1	66	theme	Process	259:265	arg1	Technology					278:287	Process Analytical Technology	259:287	Process Analytical Technology (PAT) data	259:298	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
31252145	1	66	theme	Process	259:265	arg1	PAT					290:292	PAT	290:292	PAT	290:292	This work proposes the application of artificial neural networks (ANN) to non-destructively predict the in vitro dissolution of pharmaceutical tablets from Process Analytical Technology (PAT) data.					
30717102	5	0	theme	electrophilic	717:729	arg1	function					737:744	an electrophilic enone function	714:744	an electrophilic enone function	714:744	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	0	1	from	Analysis	27:34	arg1	atra					87:90	Sea Cucumber Holothuria atra	63:90	Sea Cucumber Holothuria atra	63:90	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	6	2	theme	IC50	941:944	arg1	values					946:951	IC50 values	941:951	IC50 values ranging from 1.2 to 2.5 µg/mL	941:981	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	5	3	theme	enone	731:735	arg1	function					737:744	an electrophilic enone function	714:744	an electrophilic enone function	714:744	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	5	4	theme	side	618:621	arg1	chain					623:627	The side chain	614:627	The side chain of the new saponin 1, unprecedented among triterpene glycosides,	614:692	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	1	5	theme	Holothuria	154:163	arg1	cucumber					144:151	black sea cucumber	134:151	black sea cucumber	134:151	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	1	5	theme	Holothuria	154:163	arg1	atra					165:168	Holothuria atra	154:168	Holothuria atra collected in the Persian Gulf	154:198	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	3	6	theme	2D-NMR	514:519	arg1	experiments					521:531	1D- and 2D-NMR experiments	506:531	1D- and 2D-NMR experiments	506:531	The most abundant saponins in the extract (1⁻4) were isolated and characterized by 1D- and 2D-NMR experiments.					
30717102	1	7	theme	Molecular	255:263	arg1	Networking					265:274	Molecular Networking	255:274	Molecular Networking	255:274	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	6	8	theme	compounds	849:857	arg1	activity					837:844	The cytotoxic activity	823:844	The cytotoxic activity	823:844	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	6	8	theme	compounds	849:857	arg1	remarkable					924:933	remarkable	924:933	remarkable	924:933	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	3	9	theme	1D-	506:508	arg1	experiments					521:531	1D- and 2D-NMR experiments	506:531	1D- and 2D-NMR experiments	506:531	The most abundant saponins in the extract (1⁻4) were isolated and characterized by 1D- and 2D-NMR experiments.					
30717102	0	10	theme	Networking-Based	10:25	arg1	Analysis					27:34	Molecular Networking-Based Analysis	0:34	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.	0:91	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	6	11	theme	cell	909:912	arg1	line					914:917	the human cervix carcinoma HeLa cell line	877:917	the human cervix carcinoma HeLa cell line	877:917	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	0	12	theme	Molecular	0:8	arg1	Analysis					27:34	Molecular Networking-Based Analysis	0:34	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.	0:91	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	6	13	theme	human	881:885	arg1	line					914:917	the human cervix carcinoma HeLa cell line	877:917	the human cervix carcinoma HeLa cell line	877:917	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	1	14	theme	saponin	97:103	arg1	composition					105:115	The saponin composition	93:115	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf	93:198	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	5	15	theme	slow	765:768	arg1	water					770:774	slow water	765:774	slow water	765:774	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	4	16	theme	holothurin	599:608	arg1	A5					610:611	holothurin A5	599:611	holothurin A5	599:611	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	4	16	theme	holothurin	599:608	arg1	saponin					590:596	a new triterpene glycoside saponin	563:596	a new triterpene glycoside saponin	563:596	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	1	17	theme	pure	277:280	arg1	isolation					291:299	pure compound isolation	277:299	pure compound isolation	277:299	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	5	18	theme	unprecedented	651:663	arg1	saponin					640:646	the new saponin 1	632:648	the new saponin 1	632:648	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	6	19	theme	cytotoxic	827:835	arg1	activity					837:844	The cytotoxic activity	823:844	The cytotoxic activity	823:844	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	6	19	theme	cytotoxic	827:835	arg1	remarkable					924:933	remarkable	924:933	remarkable	924:933	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	2	20	theme	atra	362:365	arg1	complex					389:395	complex	389:395	complex	389:395	The saponin composition of Holothuria atra turned out to be more complex than previously reported.					
30717102	2	20	theme	atra	362:365	arg1	composition					336:346	The saponin composition	324:346	The saponin composition of Holothuria atra	324:365	The saponin composition of Holothuria atra turned out to be more complex than previously reported.					
30717102	0	21	theme	Saponins	49:56	arg1	Analysis					27:34	Molecular Networking-Based Analysis	0:34	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.	0:91	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	1	22	theme	compound	282:289	arg1	isolation					291:299	pure compound isolation	277:299	pure compound isolation	277:299	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	0	23	from	atra	87:90	arg1	Analysis					27:34	Molecular Networking-Based Analysis	0:34	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.	0:91	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	0	23	from	atra	87:90	arg1	Saponins					49:56	Cytotoxic Saponins	39:56	Cytotoxic Saponins from Sea Cucumber Holothuria atra	39:90	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	3	24	theme	abundant	432:439	arg1	saponins					441:448	The most abundant saponins	423:448	The most abundant saponins in the extract (1⁻4)	423:469	The most abundant saponins in the extract (1⁻4) were isolated and characterized by 1D- and 2D-NMR experiments.					
30717102	0	25	theme	Cytotoxic	39:47	arg1	Saponins					49:56	Cytotoxic Saponins	39:56	Cytotoxic Saponins from Sea Cucumber Holothuria atra	39:90	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	6	26	theme	HeLa	904:907	arg1	line					914:917	the human cervix carcinoma HeLa cell line	877:917	the human cervix carcinoma HeLa cell line	877:917	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	5	27	theme	triterpene	671:680	arg1	glycosides					682:691	triterpene glycosides	671:691	triterpene glycosides	671:691	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	5	28	theme	neutral	803:809	arg1	conditions					811:820	neutral conditions	803:820	neutral conditions	803:820	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	0	29	theme	Sea	63:65	arg1	atra					87:90	Sea Cucumber Holothuria atra	63:90	Sea Cucumber Holothuria atra	63:90	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	6	30	theme	carcinoma	894:902	arg1	line					914:917	the human cervix carcinoma HeLa cell line	877:917	the human cervix carcinoma HeLa cell line	877:917	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	3	31	from	saponins	441:448	arg1	1⁻4					466:468	1⁻4	466:468	1⁻4	466:468	The most abundant saponins in the extract (1⁻4) were isolated and characterized by 1D- and 2D-NMR experiments.					
30717102	3	31	from	saponins	441:448	arg1	extract					457:463	the extract	453:463	the extract (1⁻4)	453:469	The most abundant saponins in the extract (1⁻4) were isolated and characterized by 1D- and 2D-NMR experiments.					
30717102	2	32	theme	saponin	328:334	arg1	complex					389:395	complex	389:395	complex	389:395	The saponin composition of Holothuria atra turned out to be more complex than previously reported.					
30717102	2	32	theme	saponin	328:334	arg1	composition					336:346	The saponin composition	324:346	The saponin composition of Holothuria atra	324:365	The saponin composition of Holothuria atra turned out to be more complex than previously reported.					
30717102	6	33	theme	cervix	887:892	arg1	line					914:917	the human cervix carcinoma HeLa cell line	877:917	the human cervix carcinoma HeLa cell line	877:917	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	5	34	theme	new	636:638	arg1	saponin					640:646	the new saponin 1	632:648	the new saponin 1	632:648	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	1	35	theme	cucumber	144:151	arg1	specimen					122:129	a specimen	120:129	a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf	120:198	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	1	36	theme	specimen	122:129	arg1	composition					105:115	The saponin composition	93:115	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf	93:198	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	1	37	theme	NMR	306:308	arg1	spectroscopy					310:321	NMR spectroscopy	306:321	NMR spectroscopy	306:321	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	5	38	theme	saponin	640:646	arg1	chain					623:627	The side chain	614:627	The side chain of the new saponin 1, unprecedented among triterpene glycosides,	614:692	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	4	39	theme	triterpene	569:578	arg1	A5					610:611	holothurin A5	599:611	holothurin A5	599:611	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	4	39	theme	triterpene	569:578	arg1	Compound					534:541	Compound 1	534:543	Compound 1	534:543	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	4	39	theme	triterpene	569:578	arg1	saponin					590:596	a new triterpene glycoside saponin	563:596	a new triterpene glycoside saponin	563:596	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	6	40	with	remarkable	924:933	arg1	values					946:951	IC50 values	941:951	IC50 values ranging from 1.2 to 2.5 µg/mL	941:981	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	1	41	theme	black	134:138	arg1	cucumber					144:151	black sea cucumber	134:151	black sea cucumber	134:151	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	1	41	theme	black	134:138	arg1	atra					165:168	Holothuria atra	154:168	Holothuria atra collected in the Persian Gulf	154:198	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	4	42	theme	new	565:567	arg1	A5					610:611	holothurin A5	599:611	holothurin A5	599:611	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	4	42	theme	new	565:567	arg1	Compound					534:541	Compound 1	534:543	Compound 1	534:543	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	4	42	theme	new	565:567	arg1	saponin					590:596	a new triterpene glycoside saponin	563:596	a new triterpene glycoside saponin	563:596	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	0	43	theme	Holothuria	76:85	arg1	atra					87:90	Sea Cucumber Holothuria atra	63:90	Sea Cucumber Holothuria atra	63:90	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	5	44	theme	methanol	779:786	arg1	addition					788:795	methanol addition	779:795	methanol addition	779:795	The side chain of the new saponin 1, unprecedented among triterpene glycosides, is characterized by an electrophilic enone function, which can undergo slow water or methanol addition under neutral conditions.					
30717102	1	45	theme	sea	140:142	arg1	cucumber					144:151	black sea cucumber	134:151	black sea cucumber	134:151	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	1	45	theme	sea	140:142	arg1	atra					165:168	Holothuria atra	154:168	Holothuria atra collected in the Persian Gulf	154:198	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	1	46	theme	combined	217:224	arg1	approach					226:233	a combined approach	215:233	a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy	215:321	The saponin composition of a specimen of black sea cucumber, Holothuria atra collected in the Persian Gulf was studied by a combined approach including LC-MS/MS, Molecular Networking, pure compound isolation, and NMR spectroscopy.					
30717102	0	47	theme	Cucumber	67:74	arg1	atra					87:90	Sea Cucumber Holothuria atra	63:90	Sea Cucumber Holothuria atra	63:90	Molecular Networking-Based Analysis of Cytotoxic Saponins from Sea Cucumber Holothuria atra.					
30717102	6	48	dep	compounds	849:857	arg1	1⁻4					859:861	1⁻4	859:861	1⁻4	859:861	The cytotoxic activity of compounds 1⁻4, evaluated on the human cervix carcinoma HeLa cell line, was remarkable, with IC50 values ranging from 1.2 to 2.5 µg/mL.					
30717102	2	49	theme	Holothuria	351:360	arg1	atra					362:365	Holothuria atra	351:365	Holothuria atra	351:365	The saponin composition of Holothuria atra turned out to be more complex than previously reported.					
30717102	4	50	theme	glycoside	580:588	arg1	A5					610:611	holothurin A5	599:611	holothurin A5	599:611	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	4	50	theme	glycoside	580:588	arg1	Compound					534:541	Compound 1	534:543	Compound 1	534:543	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30717102	4	50	theme	glycoside	580:588	arg1	saponin					590:596	a new triterpene glycoside saponin	563:596	a new triterpene glycoside saponin	563:596	Compound 1 was identified as a new triterpene glycoside saponin, holothurin A5.					
30393218	7	0	theme	Cmax	1171:1174	arg1	values					1196:1201	The Cmax, t1/2 and AUC0-12 h values	1167:1201	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs	1167:1239	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	7	1	theme	drugs	1413:1417	arg1	bioavailability					1374:1388	the oral bioavailability	1365:1388	the oral bioavailability of poorlywater-soluble drugs	1365:1417	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	3	2	theme	particle	497:504	arg1	sizes					506:510	average particle sizes	489:510	average particle sizes	489:510	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	7	3	theme	h	1194:1194	arg1	values					1196:1201	The Cmax, t1/2 and AUC0-12 h values	1167:1201	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs	1167:1239	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	1	4	theme	chitosan	247:254	arg1	C-CPLs					297:302	C-CPLs	297:302	C-CPLs	297:302	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	1	4	theme	chitosan	247:254	arg1	liposomes					286:294	chitosan coated composite phospholipid liposomes	247:294	chitosan coated composite phospholipid liposomes (C-CPLs)	247:303	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	5	5	theme	particle	922:929	arg1	size					931:934	particle size	922:934	particle size of C-CA-R-CPLs	922:949	The EE of C-CA-R-CPLs of CA and R was 91.3±2.13% and 92.6±2.44%, particle size of C-CA-R-CPLs was 176.7±2.3 nm, PDI was 0.207±0.014 and zeta potential of 12.61±1.33 mV.					
30393218	1	6	theme	coated	256:261	arg1	C-CPLs					297:302	C-CPLs	297:302	C-CPLs	297:302	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	1	6	theme	coated	256:261	arg1	liposomes					286:294	chitosan coated composite phospholipid liposomes	247:294	chitosan coated composite phospholipid liposomes (C-CPLs)	247:303	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	4	7	theme	cells	740:744	arg1	model					746:750	Caco-2 cells model	733:750	Caco-2 cells model	733:750	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	4	8	from	pharmacokinetics	760:775	arg1	rats					800:803	Sprague-Dawley (SD) rats	780:803	Sprague-Dawley (SD) rats	780:803	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	5	9	theme	CA	882:883	arg1	C-CA-R-CPLs					867:877	C-CA-R-CPLs	867:877	C-CA-R-CPLs of CA and R	867:889	The EE of C-CA-R-CPLs of CA and R was 91.3±2.13% and 92.6±2.44%, particle size of C-CA-R-CPLs was 176.7±2.3 nm, PDI was 0.207±0.014 and zeta potential of 12.61±1.33 mV.					
30393218	1	10	theme	composite	263:271	arg1	C-CPLs					297:302	C-CPLs	297:302	C-CPLs	297:302	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	1	10	theme	composite	263:271	arg1	liposomes					286:294	chitosan coated composite phospholipid liposomes	247:294	chitosan coated composite phospholipid liposomes (C-CPLs)	247:303	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	7	11	theme	R	1213:1213	arg1	values					1196:1201	The Cmax, t1/2 and AUC0-12 h values	1167:1201	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs	1167:1239	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	4	12	theme	vein	835:838	arg1	tube					851:854	rat jugular vein intubation tube	823:854	rat jugular vein intubation tube	823:854	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	3	13	theme	disperse	521:528	arg1	PDI					537:539	PDI	537:539	PDI	537:539	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	3	13	theme	disperse	521:528	arg1	index					530:534	polymer disperse index	513:534	polymer disperse index (PDI)	513:540	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	7	14	dep	nano-formulation	1311:1326	arg1	have					1328:1331	have	1328:1331	have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration	1328:1443	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	7	15	theme	poorlywater-soluble	1393:1411	arg1	drugs					1413:1417	poorlywater-soluble drugs	1393:1417	poorlywater-soluble drugs	1393:1417	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	4	16	theme	jugular	827:833	arg1	tube					851:854	rat jugular vein intubation tube	823:854	rat jugular vein intubation tube	823:854	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	5	17	theme	C-CA-R-CPLs	939:949	arg1	size					931:934	particle size	922:934	particle size of C-CA-R-CPLs	922:949	The EE of C-CA-R-CPLs of CA and R was 91.3±2.13% and 92.6±2.44%, particle size of C-CA-R-CPLs was 176.7±2.3 nm, PDI was 0.207±0.014 and zeta potential of 12.61±1.33 mV.					
30393218	1	18	theme	chlorogenic	194:204	arg1	acid					206:209	chlorogenic acid	194:209	chlorogenic acid	194:209	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	6	19	theme	CPL	1111:1113	arg1	bilayer					1096:1102	the bilayer	1092:1102	the bilayer of the CPL	1092:1113	CA-R-CPLs and C-CA-R-CPLs were spherical or elliptical sphere and the bilayer of the CPL was observed obviously under transmission electron.					
30393218	3	20	theme	zeta	543:546	arg1	potential					548:556	zeta potential	543:556	zeta potential	543:556	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	7	21	theme	oral	1425:1428	arg1	administration					1430:1443	oral administration	1425:1443	oral administration	1425:1443	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	1	22	theme	acid	206:209	arg1	bioavailability					175:189	bioavailability	175:189	bioavailability of chlorogenic acid and rutin(CA-R)	175:225	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	2	23	theme	injection	446:454	arg1	method					428:433	the method	424:433	the method of ethanol injection	424:454	TheC-CA-R-CPLs were prepared by the method of ethanol injection.					
30393218	1	24	theme	phospholipid	273:284	arg1	C-CPLs					297:302	C-CPLs	297:302	C-CPLs	297:302	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	1	24	theme	phospholipid	273:284	arg1	liposomes					286:294	chitosan coated composite phospholipid liposomes	247:294	chitosan coated composite phospholipid liposomes (C-CPLs)	247:303	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	4	25	theme	intubation	840:849	arg1	tube					851:854	rat jugular vein intubation tube	823:854	rat jugular vein intubation tube	823:854	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	4	26	theme	Caco-2	733:738	arg1	cells					740:744	Caco-2 cells	733:744	Caco-2 cells model	733:750	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	3	27	dep	in	569:570	arg1	vitro					572:576	vitro	572:576	vitro	572:576	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	2	28	theme	ethanol	438:444	arg1	injection					446:454	ethanol injection	438:454	ethanol injection	438:454	TheC-CA-R-CPLs were prepared by the method of ethanol injection.					
30393218	5	29	theme	zeta	993:996	arg1	potential					998:1006	zeta potential	993:1006	zeta potential	993:1006	The EE of C-CA-R-CPLs of CA and R was 91.3±2.13% and 92.6±2.44%, particle size of C-CA-R-CPLs was 176.7±2.3 nm, PDI was 0.207±0.014 and zeta potential of 12.61±1.33 mV.					
30393218	1	30	theme	rutin	215:219	arg1	bioavailability					175:189	bioavailability	175:189	bioavailability of chlorogenic acid and rutin(CA-R)	175:225	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	0	31	theme	chlorogenic	16:26	arg1	acid					28:31	chlorogenic acid	16:31	chlorogenic acid	16:31	Chitosan coated chlorogenic acid and rutincomposite phospholipid liposomes: Preparation, characterizations, permeability and pharmacokinetic.					
30393218	4	32	theme	Sprague-Dawley	780:793	arg1	rats					800:803	Sprague-Dawley (SD) rats	780:803	Sprague-Dawley (SD) rats	780:803	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	1	33	theme	C-CA-R-CPLs	379:389	arg1	preparation					330:340	preparation	330:340	preparation	330:340	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	1	33	theme	C-CA-R-CPLs	379:389	arg1	permeability					343:354	permeability	343:354	permeability	343:354	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	5	34	theme	C-CA-R-CPLs	867:877	arg1	EE					861:862	The EE	857:862	The EE of C-CA-R-CPLs of CA and R	857:889	The EE of C-CA-R-CPLs of CA and R was 91.3±2.13% and 92.6±2.44%, particle size of C-CA-R-CPLs was 176.7±2.3 nm, PDI was 0.207±0.014 and zeta potential of 12.61±1.33 mV.					
30393218	5	34	theme	C-CA-R-CPLs	867:877	arg1	%					904:904	91.3±2.13%	895:904	91.3±2.13%	895:904	The EE of C-CA-R-CPLs of CA and R was 91.3±2.13% and 92.6±2.44%, particle size of C-CA-R-CPLs was 176.7±2.3 nm, PDI was 0.207±0.014 and zeta potential of 12.61±1.33 mV.					
30393218	0	35	theme	rutincomposite	37:50	arg1	liposomes					65:73	rutincomposite phospholipid liposomes	37:73	rutincomposite phospholipid liposomes	37:73	Chitosan coated chlorogenic acid and rutincomposite phospholipid liposomes: Preparation, characterizations, permeability and pharmacokinetic.					
30393218	3	36	theme	physicochemical	625:639	arg1	parameters					641:650	physicochemical parameters	625:650	physicochemical parameters of C-CA-R-CPLs	625:665	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	3	37	theme	in	569:570	arg1	release					583:589	in vitro drug release	569:589	in vitro drug release	569:589	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	3	38	theme	entrapment	461:470	arg1	EE					484:485	EE	484:485	EE	484:485	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	3	38	theme	entrapment	461:470	arg1	efficiency					472:481	The entrapment efficiency	457:481	The entrapment efficiency (EE)	457:486	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	5	39	theme	mV	1022:1023	arg1	0.207±0.014					977:987	0.207±0.014	977:987	0.207±0.014	977:987	The EE of C-CA-R-CPLs of CA and R was 91.3±2.13% and 92.6±2.44%, particle size of C-CA-R-CPLs was 176.7±2.3 nm, PDI was 0.207±0.014 and zeta potential of 12.61±1.33 mV.					
30393218	5	39	theme	mV	1022:1023	arg1	potential					998:1006	zeta potential	993:1006	zeta potential	993:1006	The EE of C-CA-R-CPLs of CA and R was 91.3±2.13% and 92.6±2.44%, particle size of C-CA-R-CPLs was 176.7±2.3 nm, PDI was 0.207±0.014 and zeta potential of 12.61±1.33 mV.					
30393218	4	40	theme	rat	823:825	arg1	tube					851:854	rat jugular vein intubation tube	823:854	rat jugular vein intubation tube	823:854	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	5	41	theme	R	889:889	arg1	C-CA-R-CPLs					867:877	C-CA-R-CPLs	867:877	C-CA-R-CPLs of CA and R	867:889	The EE of C-CA-R-CPLs of CA and R was 91.3±2.13% and 92.6±2.44%, particle size of C-CA-R-CPLs was 176.7±2.3 nm, PDI was 0.207±0.014 and zeta potential of 12.61±1.33 mV.					
30393218	0	42	theme	phospholipid	52:63	arg1	liposomes					65:73	rutincomposite phospholipid liposomes	37:73	rutincomposite phospholipid liposomes	37:73	Chitosan coated chlorogenic acid and rutincomposite phospholipid liposomes: Preparation, characterizations, permeability and pharmacokinetic.					
30393218	3	43	theme	C-CA-R-CPLs	655:665	arg1	parameters					641:650	physicochemical parameters	625:650	physicochemical parameters of C-CA-R-CPLs	625:665	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	6	44	dep	spherical	1057:1065	arg1	sphere					1081:1086	sphere	1081:1086	sphere	1081:1086	CA-R-CPLs and C-CA-R-CPLs were spherical or elliptical sphere and the bilayer of the CPL was observed obviously under transmission electron.					
30393218	3	45	theme	average	489:495	arg1	sizes					506:510	average particle sizes	489:510	average particle sizes	489:510	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	6	46	theme	transmission	1144:1155	arg1	electron					1157:1164	transmission electron	1144:1164	transmission electron	1144:1164	CA-R-CPLs and C-CA-R-CPLs were spherical or elliptical sphere and the bilayer of the CPL was observed obviously under transmission electron.					
30393218	7	47	theme	CA	1206:1207	arg1	values					1196:1201	The Cmax, t1/2 and AUC0-12 h values	1167:1201	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs	1167:1239	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	7	48	theme	oral	1369:1372	arg1	bioavailability					1374:1388	the oral bioavailability	1365:1388	the oral bioavailability of poorlywater-soluble drugs	1365:1417	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	7	49	theme	novel	1305:1309	arg1	conclusion					1273:1282	conclusion	1273:1282	conclusion	1273:1282	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	7	49	theme	novel	1305:1309	arg1	nano-formulation					1311:1326	a novel nano-formulation	1303:1326	a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration	1303:1443	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	7	50	theme	t1/2	1177:1180	arg1	values					1196:1201	The Cmax, t1/2 and AUC0-12 h values	1167:1201	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs	1167:1239	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	0	51	dep	coated	9:14	arg1	permeability					108:119	permeability	108:119	permeability	108:119	Chitosan coated chlorogenic acid and rutincomposite phospholipid liposomes: Preparation, characterizations, permeability and pharmacokinetic.					
30393218	0	51	dep	coated	9:14	arg1	characterizations					89:105	characterizations	89:105	characterizations	89:105	Chitosan coated chlorogenic acid and rutincomposite phospholipid liposomes: Preparation, characterizations, permeability and pharmacokinetic.					
30393218	0	51	dep	coated	9:14	arg1	Preparation					76:86	Preparation	76:86	Preparation	76:86	Chitosan coated chlorogenic acid and rutincomposite phospholipid liposomes: Preparation, characterizations, permeability and pharmacokinetic.					
30393218	0	51	dep	coated	9:14	arg1	pharmacokinetic					125:139	pharmacokinetic	125:139	pharmacokinetic	125:139	Chitosan coated chlorogenic acid and rutincomposite phospholipid liposomes: Preparation, characterizations, permeability and pharmacokinetic.					
30393218	7	52	theme	C-CA-R-CPLs	1229:1239	arg1	C-CA-R-CPLs					1229:1239	C-CA-R-CPLs	1229:1239	C-CA-R-CPLs	1229:1239	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	7	52	theme	C-CA-R-CPLs	1229:1239	arg1	groups					1219:1224	groups	1219:1224	groups of C-CA-R-CPLs	1219:1239	The Cmax, t1/2 and AUC0-12 h values of CA and R for groups of C-CA-R-CPLs were significantly increased.In conclusion, TheC-CA-R-CPLs as a novel nano-formulation have potential to be used to enhance the oral bioavailability of poorlywater-soluble drugs after oral administration.					
30393218	4	53	theme	penetration	672:682	arg1	properties					684:693	The penetration properties	668:693	The penetration properties from C-CA-R-CPLs	668:710	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	4	54	from	C-CA-R-CPLs	700:710	arg1	properties					684:693	The penetration properties	668:693	The penetration properties from C-CA-R-CPLs	668:710	The penetration properties from C-CA-R-CPLs were studied through Caco-2 cells model and the pharmacokinetics in Sprague-Dawley (SD) rats were evaluated by rat jugular vein intubation tube.					
30393218	1	55	theme	oral	235:238	arg1	route					240:244	the oral route	231:244	the oral route	231:244	In order to research and enhance bioavailability of chlorogenic acid and rutin(CA-R) via the oral route, chitosan coated composite phospholipid liposomes (C-CPLs) were applied to study on preparation, permeability and pharmacokinetic of C-CA-R-CPLs.					
30393218	3	56	theme	drug	578:581	arg1	release					583:589	in vitro drug release	569:589	in vitro drug release	569:589	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	3	57	theme	polymer	513:519	arg1	PDI					537:539	PDI	537:539	PDI	537:539	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
30393218	3	57	theme	polymer	513:519	arg1	index					530:534	polymer disperse index	513:534	polymer disperse index (PDI)	513:540	The entrapment efficiency (EE), average particle sizes, polymer disperse index (PDI), zeta potential, shape and in vitro drug release were investigated to characterize physicochemical parameters of C-CA-R-CPLs.					
31751707	8	0	from	sectors	1575:1581	arg1	industries					1554:1563	the food and pharmaceutical industries	1526:1563	the food and pharmaceutical industries	1526:1563	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	1	theme	molecular	1430:1438	arg1	weight					1440:1445	its molecular weight	1426:1445	its molecular weight	1426:1445	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	2	2	theme	bovine	398:403	arg1	BCS					422:424	BCS	422:424	BCS	422:424	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	2	2	theme	bovine	398:403	arg1	cartilage					411:419	bovine nasal cartilage	398:419	bovine nasal cartilage (BCS)	398:425	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	0	3	theme	nasal	81:85	arg1	cartilage					87:95	bovine nasal cartilage	74:95	bovine nasal cartilage	74:95	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	5	4	theme	BCS-II	900:905	arg1	activities					886:895	antioxidant activities	874:895	antioxidant activities of BCS-II and its subfractions BCS-II-1-4	874:937	Finally, antioxidant activities of BCS-II and its subfractions BCS-II-1-4 were compared.					
31751707	6	5	theme	functional	1025:1034	arg1	BCS-II					1045:1050	BCS-II	1045:1050	BCS-II	1045:1050	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	6	5	theme	functional	1025:1034	arg1	group					1036:1040	the primary functional group	1013:1040	the primary functional group of BCS-II	1013:1050	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	2	6	theme	antioxidant	319:329	arg1	activity					331:338	antioxidant activity	319:338	antioxidant activity	319:338	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	6	7	theme	H2O2-Vc	982:988	arg1	system					990:995	the H2O2-Vc system	978:995	the H2O2-Vc system	978:995	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	6	8	from	change	1073:1078	arg1	content					1091:1097	sulfate content	1083:1097	sulfate content between BCS-II and its degraded fractions	1083:1139	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	6	9	theme	sulfate	1083:1089	arg1	content					1091:1097	sulfate content	1083:1097	sulfate content between BCS-II and its degraded fractions	1083:1139	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	5	10	theme	BCS-II-1-4	928:937	arg1	activities					886:895	antioxidant activities	874:895	antioxidant activities of BCS-II and its subfractions BCS-II-1-4	874:937	Finally, antioxidant activities of BCS-II and its subfractions BCS-II-1-4 were compared.					
31751707	2	11	attach	derived	385:391	arg2	weights					354:360	molecular weights	344:360	molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS)	344:425	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	2	11	attach	derived	385:391	arg1	BCS					422:424	BCS	422:424	BCS	422:424	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	2	11	attach	derived	385:391	arg1	cartilage					411:419	bovine nasal cartilage	398:419	bovine nasal cartilage (BCS)	398:425	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	6	12	theme	BCS-II	1045:1050	arg1	BCS-II					1045:1050	BCS-II	1045:1050	BCS-II	1045:1050	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	6	12	theme	BCS-II	1045:1050	arg1	group					1036:1040	the primary functional group	1013:1040	the primary functional group of BCS-II	1013:1050	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	6	13	theme	acid	1158:1161	arg1	levels					1163:1168	uronic acid levels	1151:1168	uronic acid levels	1151:1168	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	7	14	theme	strongest	1298:1306	arg1	activity					1320:1327	the strongest antioxidant activity	1294:1327	the strongest antioxidant activity	1294:1327	In vitro, BCS-II-4 displayed the lowest molecular weight and had the strongest antioxidant activity.					
31751707	0	15	theme	antioxidant	107:117	arg1	activities					119:128	their antioxidant activities	101:128	their antioxidant activities	101:128	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	4	16	theme	BCS-II	759:764	arg1	properties					745:754	the physicochemical properties	725:754	the physicochemical properties of BCS-II before and after degradation	725:793	Changes in the physicochemical properties of BCS-II before and after degradation were compared via FT-IR, NMR and monosaccharide composition analysis.					
31751707	3	17	dep	subfractions	612:623	arg1	19.7 kDa					703:710	19.7 kDa	703:710	19.7 kDa	703:710	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	3	17	dep	subfractions	612:623	arg1	BCS-II-2					647:654	BCS-II-2	647:654	BCS-II-2 (54.1 kDa)	647:665	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	3	17	dep	subfractions	612:623	arg1	BCS-II-1					626:633	BCS-II-1	626:633	BCS-II-1 (92.7 kDa)	626:644	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	3	17	dep	subfractions	612:623	arg1	54.1 kDa					657:664	54.1 kDa	657:664	54.1 kDa	657:664	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	3	17	dep	subfractions	612:623	arg1	92.7 kDa					636:643	92.7 kDa	636:643	92.7 kDa	636:643	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	3	17	dep	subfractions	612:623	arg1	BCS-II-4					693:700	BCS-II-4	693:700	BCS-II-4 (19.7 kDa)	693:711	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	3	17	dep	subfractions	612:623	arg1	BCS-II-3					668:675	BCS-II-3 (26.3 kDa)	668:686	BCS-II-3 (26.3 kDa)	668:686	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	3	17	dep	subfractions	612:623	arg1	subfractions					612:623	four subfractions	607:623	four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa)	607:711	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	8	18	from	antioxidant	1511:1521	arg1	industries					1554:1563	the food and pharmaceutical industries	1526:1563	the food and pharmaceutical industries	1526:1563	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	19	theme	food	1530:1533	arg1	industries					1554:1563	the food and pharmaceutical industries	1526:1563	the food and pharmaceutical industries	1526:1563	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	4	20	theme	composition	843:853	arg1	analysis					855:862	monosaccharide composition analysis	828:862	monosaccharide composition analysis	828:862	Changes in the physicochemical properties of BCS-II before and after degradation were compared via FT-IR, NMR and monosaccharide composition analysis.					
31751707	6	21	dep	disrupt	1005:1011	arg1	increased					1170:1178	increased	1170:1178	increased in degraded fractions when compared with BCS-II	1170:1226	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	3	22	theme	enzymatic	454:462	arg1	method					464:469	compound enzymatic method	445:469	compound enzymatic method	445:469	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	6	23	theme	primary	1017:1023	arg1	BCS-II					1045:1050	BCS-II	1045:1050	BCS-II	1045:1050	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	6	23	theme	primary	1017:1023	arg1	group					1036:1040	the primary functional group	1013:1040	the primary functional group of BCS-II	1013:1050	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	4	24	theme	monosaccharide	828:841	arg1	analysis					855:862	monosaccharide composition analysis	828:862	monosaccharide composition analysis	828:862	Changes in the physicochemical properties of BCS-II before and after degradation were compared via FT-IR, NMR and monosaccharide composition analysis.					
31751707	2	25	theme	molecular	344:352	arg1	weights					354:360	molecular weights	344:360	molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS)	344:425	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	7	26	theme	antioxidant	1308:1318	arg1	activity					1320:1327	the strongest antioxidant activity	1294:1327	the strongest antioxidant activity	1294:1327	In vitro, BCS-II-4 displayed the lowest molecular weight and had the strongest antioxidant activity.					
31751707	0	27	theme	sulfates	27:34	arg1	Preparation					0:10	Preparation	0:10	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.	0:129	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	8	28	theme	pharmaceutical	1539:1552	arg1	industries					1554:1563	the food and pharmaceutical industries	1526:1563	the food and pharmaceutical industries	1526:1563	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	4	29	from	Changes	714:720	arg1	properties					745:754	the physicochemical properties	725:754	the physicochemical properties of BCS-II before and after degradation	725:793	Changes in the physicochemical properties of BCS-II before and after degradation were compared via FT-IR, NMR and monosaccharide composition analysis.					
31751707	4	30	theme	physicochemical	729:743	arg1	properties					745:754	the physicochemical properties	725:754	the physicochemical properties of BCS-II before and after degradation	725:793	Changes in the physicochemical properties of BCS-II before and after degradation were compared via FT-IR, NMR and monosaccharide composition analysis.					
31751707	3	31	theme	DEAE-cellulose	496:509	arg1	separation					518:527	DEAE-cellulose column separation	496:527	DEAE-cellulose column separation	496:527	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	0	32	theme	chondroitin	15:25	arg1	sulfates					27:34	chondroitin sulfates	15:34	chondroitin sulfates	15:34	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	8	33	theme	other	1569:1573	arg1	sulfate					1485:1491	low-molecular-weight chondroitin sulfate	1452:1491	low-molecular-weight chondroitin sulfate	1452:1491	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	33	theme	other	1569:1573	arg1	sectors					1575:1581	other sectors	1569:1581	other sectors	1569:1581	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	34	theme	antioxidant	1345:1355	arg1	activity					1357:1364	the antioxidant activity	1341:1364	the antioxidant activity of chondroitin sulfate in vitro	1341:1396	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	0	35	from	cartilage	87:95	arg1	weights					61:67	different molecular weights	41:67	different molecular weights from bovine nasal cartilage and their antioxidant activities	41:128	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	8	36	theme	chondroitin	1369:1379	arg1	sulfate					1381:1387	chondroitin sulfate	1369:1387	chondroitin sulfate	1369:1387	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	3	37	theme	column	511:516	arg1	separation					518:527	DEAE-cellulose column separation	496:527	DEAE-cellulose column separation	496:527	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	0	38	theme	different	41:49	arg1	weights					61:67	different molecular weights	41:67	different molecular weights from bovine nasal cartilage and their antioxidant activities	41:128	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	2	39	theme	sulfate	377:383	arg1	activity					331:338	antioxidant activity	319:338	antioxidant activity	319:338	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	2	39	theme	sulfate	377:383	arg1	weights					354:360	molecular weights	344:360	molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS)	344:425	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	3	40	theme	compound	445:452	arg1	method					464:469	compound enzymatic method	445:469	compound enzymatic method	445:469	BCS extracted by compound enzymatic method was further purified via DEAE-cellulose column separation to obtain BCS-II (129.4 kDa), which was further degraded by H2O2-Vc to obtain four subfractions: BCS-II-1 (92.7 kDa), BCS-II-2 (54.1 kDa), BCS-II-3 (26.3 kDa), and BCS-II-4 (19.7 kDa).					
31751707	8	41	theme	low-molecular-weight	1452:1471	arg1	sulfate					1485:1491	low-molecular-weight chondroitin sulfate	1452:1491	low-molecular-weight chondroitin sulfate	1452:1491	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	41	theme	low-molecular-weight	1452:1471	arg1	antioxidant					1511:1521	an antioxidant	1508:1521	an antioxidant in the food and pharmaceutical industries	1508:1563	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	41	theme	low-molecular-weight	1452:1471	arg1	sectors					1575:1581	other sectors	1569:1581	other sectors	1569:1581	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	5	42	theme	antioxidant	874:884	arg1	activities					886:895	antioxidant activities	874:895	antioxidant activities of BCS-II and its subfractions BCS-II-1-4	874:937	Finally, antioxidant activities of BCS-II and its subfractions BCS-II-1-4 were compared.					
31751707	7	43	contain	had	1290:1292	arg2	activity					1320:1327	the strongest antioxidant activity	1294:1327	the strongest antioxidant activity	1294:1327	In vitro, BCS-II-4 displayed the lowest molecular weight and had the strongest antioxidant activity.					
31751707	7	43	contain	had	1290:1292	arg1	BCS-II-4					1239:1246	BCS-II-4	1239:1246	BCS-II-4	1239:1246	In vitro, BCS-II-4 displayed the lowest molecular weight and had the strongest antioxidant activity.					
31751707	6	44	theme	degraded	1122:1129	arg1	fractions					1131:1139	its degraded fractions	1118:1139	its degraded fractions	1118:1139	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	0	45	theme	molecular	51:59	arg1	weights					61:67	different molecular weights	41:67	different molecular weights from bovine nasal cartilage and their antioxidant activities	41:128	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	0	46	from	activities	119:128	arg1	weights					61:67	different molecular weights	41:67	different molecular weights from bovine nasal cartilage and their antioxidant activities	41:128	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	7	47	theme	lowest	1262:1267	arg1	weight					1279:1284	the lowest molecular weight	1258:1284	the lowest molecular weight	1258:1284	In vitro, BCS-II-4 displayed the lowest molecular weight and had the strongest antioxidant activity.					
31751707	1	48	theme	Biological	131:140	arg1	functions					142:150	Biological functions	131:150	Biological functions	131:150	Biological functions of chondroitin sulfate, including anti-oxidation and anti-inflammation, are associated with its molecular weight.					
31751707	1	48	theme	Biological	131:140	arg1	anti-inflammation					205:221	anti-inflammation	205:221	anti-inflammation	205:221	Biological functions of chondroitin sulfate, including anti-oxidation and anti-inflammation, are associated with its molecular weight.					
31751707	1	48	theme	Biological	131:140	arg1	anti-oxidation					186:199	anti-oxidation	186:199	anti-oxidation	186:199	Biological functions of chondroitin sulfate, including anti-oxidation and anti-inflammation, are associated with its molecular weight.					
31751707	8	49	theme	chondroitin	1473:1483	arg1	sulfate					1485:1491	low-molecular-weight chondroitin sulfate	1452:1491	low-molecular-weight chondroitin sulfate	1452:1491	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	49	theme	chondroitin	1473:1483	arg1	antioxidant					1511:1521	an antioxidant	1508:1521	an antioxidant in the food and pharmaceutical industries	1508:1563	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	49	theme	chondroitin	1473:1483	arg1	sectors					1575:1581	other sectors	1569:1581	other sectors	1569:1581	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	0	50	with	Preparation	0:10	arg1	weights					61:67	different molecular weights	41:67	different molecular weights from bovine nasal cartilage and their antioxidant activities	41:128	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	2	51	theme	chondroitin	365:375	arg1	sulfate					377:383	chondroitin sulfate	365:383	chondroitin sulfate	365:383	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	8	52	theme	sulfate	1381:1387	arg1	activity					1357:1364	the antioxidant activity	1341:1364	the antioxidant activity of chondroitin sulfate in vitro	1341:1396	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	1	53	theme	molecular	248:256	arg1	weight					258:263	its molecular weight	244:263	its molecular weight	244:263	Biological functions of chondroitin sulfate, including anti-oxidation and anti-inflammation, are associated with its molecular weight.					
31751707	6	54	theme	uronic	1151:1156	arg1	levels					1163:1168	uronic acid levels	1151:1168	uronic acid levels	1151:1168	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	1	55	theme	sulfate	167:173	arg1	functions					142:150	Biological functions	131:150	Biological functions	131:150	Biological functions of chondroitin sulfate, including anti-oxidation and anti-inflammation, are associated with its molecular weight.					
31751707	1	55	theme	sulfate	167:173	arg1	anti-inflammation					205:221	anti-inflammation	205:221	anti-inflammation	205:221	Biological functions of chondroitin sulfate, including anti-oxidation and anti-inflammation, are associated with its molecular weight.					
31751707	1	55	theme	sulfate	167:173	arg1	anti-oxidation					186:199	anti-oxidation	186:199	anti-oxidation	186:199	Biological functions of chondroitin sulfate, including anti-oxidation and anti-inflammation, are associated with its molecular weight.					
31751707	5	56	theme	subfractions	915:926	arg1	BCS-II-1-4					928:937	its subfractions BCS-II-1-4	911:937	its subfractions BCS-II-1-4	911:937	Finally, antioxidant activities of BCS-II and its subfractions BCS-II-1-4 were compared.					
31751707	0	57	theme	bovine	74:79	arg1	cartilage					87:95	bovine nasal cartilage	74:95	bovine nasal cartilage	74:95	Preparation of chondroitin sulfates with different molecular weights from bovine nasal cartilage and their antioxidant activities.					
31751707	6	58	theme	degraded	1183:1190	arg1	fractions					1192:1200	degraded fractions	1183:1200	degraded fractions	1183:1200	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	1	59	theme	chondroitin	155:165	arg1	sulfate					167:173	chondroitin sulfate	155:173	chondroitin sulfate	155:173	Biological functions of chondroitin sulfate, including anti-oxidation and anti-inflammation, are associated with its molecular weight.					
31751707	6	60	theme	significant	1061:1071	arg1	change					1073:1078	no significant change	1058:1078	no significant change in sulfate content between BCS-II and its degraded fractions	1058:1139	Our results showed that the H2O2-Vc system did not disrupt the primary functional group of BCS-II, with no significant change in sulfate content between BCS-II and its degraded fractions; however, uronic acid levels increased in degraded fractions when compared with BCS-II.					
31751707	8	61	used	used	1500:1503	arg2	sulfate					1485:1491	low-molecular-weight chondroitin sulfate	1452:1491	low-molecular-weight chondroitin sulfate	1452:1491	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	61	used	used	1500:1503	arg2	antioxidant					1511:1521	an antioxidant	1508:1521	an antioxidant in the food and pharmaceutical industries	1508:1563	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	8	61	used	used	1500:1503	arg2	sectors					1575:1581	other sectors	1569:1581	other sectors	1569:1581	Therefore, the antioxidant activity of chondroitin sulfate in vitro is robustly associated with its molecular weight, and low-molecular-weight chondroitin sulfate can be used as an antioxidant in the food and pharmaceutical industries and other sectors.					
31751707	7	62	theme	molecular	1269:1277	arg1	weight					1279:1284	the lowest molecular weight	1258:1284	the lowest molecular weight	1258:1284	In vitro, BCS-II-4 displayed the lowest molecular weight and had the strongest antioxidant activity.					
31751707	2	63	theme	nasal	405:409	arg1	BCS					422:424	BCS	422:424	BCS	422:424	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
31751707	2	63	theme	nasal	405:409	arg1	cartilage					411:419	bovine nasal cartilage	398:419	bovine nasal cartilage (BCS)	398:425	This study aimed to evaluate the correlation between antioxidant activity and molecular weights of chondroitin sulfate derived from bovine nasal cartilage (BCS).					
29524801	6	0	from	Variation	866:874	arg1	growth					889:894	growth	889:894	growth	889:894	RESULTS Variation in survival, growth and mineral composition was found between species.					
29524801	6	0	from	Variation	866:874	arg1	composition					908:918	mineral composition	900:918	mineral composition	900:918	RESULTS Variation in survival, growth and mineral composition was found between species.					
29524801	6	0	from	Variation	866:874	arg1	survival					879:886	survival	879:886	survival	879:886	RESULTS Variation in survival, growth and mineral composition was found between species.					
29524801	0	1	theme	species	148:154	arg1	roots					129:133	the roots	125:133	the roots of two Erica species	125:154	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	12	2	dep	CONCLUSIONS	1476:1486	arg1	Variation					1488:1496	Variation	1488:1496	Variation in elements linked to the cell wall-membrane complex	1488:1549	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	12	2	dep	CONCLUSIONS	1476:1486	arg1	stability					1559:1567	the stability	1555:1567	the stability of their components (RG-II, H+-ATPases)	1555:1607	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	12	2	dep	CONCLUSIONS	1476:1486	arg1	CONCLUSIONS					1476:1486	CONCLUSIONS	1476:1486	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases)	1476:1607	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	11	3	theme	pH	1403:1404	arg1	medium					1393:1398	a medium	1391:1398	a medium of pH 2	1391:1406	However, shifting to a medium of pH 2 caused some of the boron bridges to break in the sensitive species.					
29524801	1	4	theme	Ericaceae	233:241	arg1	species					222:228	two species	218:228	two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location	218:419	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	8	5	theme	root	1103:1106	arg1	nutrients					1108:1116	root nutrients	1103:1116	root nutrients (K, P, Mg)	1103:1127	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	11	6	theme	boron	1427:1431	arg1	bridges					1433:1439	the boron bridges	1423:1439	the boron bridges	1423:1439	However, shifting to a medium of pH 2 caused some of the boron bridges to break in the sensitive species.					
29524801	0	7	theme	Active	0:5	arg1	efflux					14:19	Active proton efflux	0:19	Active proton efflux	0:19	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	13	8	theme	root	1669:1672	arg1	structure					1679:1687	root cell structure	1669:1687	root cell structure	1669:1687	Thus, by maintaining root cell structure, active proton efflux avoided toxic H+ build-up in the cytoplasm and supported greater nutrient acquisition in H+-tolerant species.					
29524801	2	9	theme	SW	513:514	arg1	Spain					516:520	SW Spain	513:520	SW Spain	513:520	METHODS Tolerance to H+ toxicity was tested in nutrient solutions using seeds collected in SW Spain.					
29524801	4	10	theme	Viability	602:610	arg1	tests					612:616	Viability tests	602:616	Viability tests	602:616	Viability tests and proton exchange were studied in roots exposed, short-term, to acidic conditions.					
29524801	0	11	theme	proton	106:111	arg1	toxicity					113:120	proton toxicity	106:120	proton toxicity	106:120	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	6	12	theme	mineral	900:906	arg1	composition					908:918	mineral composition	900:918	mineral composition	900:918	RESULTS Variation in survival, growth and mineral composition was found between species.					
29524801	1	13	theme	interspecific	328:340	arg1	variations					342:351	interspecific variations	328:351	interspecific variations in H+ tolerance which might be related to their particular location	328:419	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	1	14	theme	soil	190:193	arg1	acidity					195:201	soil acidity	190:201	soil acidity	190:201	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	7	15	theme	greater	999:1005	arg1	concentration					1007:1019	greater concentration	999:1019	greater concentration of nutrients	999:1032	The H+-tolerant species (Erica andevalensis) showed greater concentration of nutrients than E. australis.					
29524801	3	16	theme	Plant	523:527	arg1	growth					529:534	Plant growth	523:534	Plant growth	523:534	Plant growth and nutrient contents in leaves, stems and roots were determined.					
29524801	7	17	theme	H+-tolerant	951:961	arg1	species					963:969	The H+-tolerant species	947:969	The H+-tolerant species (Erica andevalensis)	947:990	The H+-tolerant species (Erica andevalensis) showed greater concentration of nutrients than E. australis.					
29524801	7	17	theme	H+-tolerant	951:961	arg1	andevalensis					978:989	Erica andevalensis	972:989	Erica andevalensis	972:989	The H+-tolerant species (Erica andevalensis) showed greater concentration of nutrients than E. australis.					
29524801	0	18	from	tolerance	93:101	arg1	roots					129:133	the roots	125:133	the roots of two Erica species	125:154	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	12	19	from	stability	1559:1567	arg1	elements					1501:1508	elements	1501:1508	elements linked to the cell wall-membrane complex	1501:1549	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	9	20	theme	ATPase	1160:1165	arg1	activity					1167:1174	Root ATPase activity	1155:1174	Root ATPase activity	1155:1174	Root ATPase activity was slightly higher in the tolerant species with a correspondingly greater H+ efflux capacity.					
29524801	4	21	dep	exposed	660:666	arg1	short-term					669:678	short-term	669:678	short-term	669:678	Viability tests and proton exchange were studied in roots exposed, short-term, to acidic conditions.					
29524801	2	22	theme	H+	443:444	arg1	toxicity					446:453	H+ toxicity	443:453	H+ toxicity	443:453	METHODS Tolerance to H+ toxicity was tested in nutrient solutions using seeds collected in SW Spain.					
29524801	13	23	theme	greater	1768:1774	arg1	acquisition					1785:1795	greater nutrient acquisition	1768:1795	greater nutrient acquisition	1768:1795	Thus, by maintaining root cell structure, active proton efflux avoided toxic H+ build-up in the cytoplasm and supported greater nutrient acquisition in H+-tolerant species.					
29524801	10	24	theme	domains	1320:1326	arg1	majority					1298:1305	the great majority	1288:1305	the great majority of the RG-II domains	1288:1326	In both species, the great majority of the RG-II domains were in their boron-bridged dimeric form.					
29524801	4	25	theme	acidic	684:689	arg1	conditions					691:700	acidic conditions	684:700	acidic conditions	684:700	Viability tests and proton exchange were studied in roots exposed, short-term, to acidic conditions.					
29524801	1	26	from	variations	342:351	arg1	tolerance					359:367	H+ tolerance	356:367	H+ tolerance	356:367	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	3	27	from	contents	549:556	arg1	roots					579:583	roots	579:583	roots	579:583	Plant growth and nutrient contents in leaves, stems and roots were determined.					
29524801	3	27	from	contents	549:556	arg1	stems					569:573	stems	569:573	stems	569:573	Plant growth and nutrient contents in leaves, stems and roots were determined.					
29524801	3	27	from	contents	549:556	arg1	leaves					561:566	leaves	561:566	leaves	561:566	Plant growth and nutrient contents in leaves, stems and roots were determined.					
29524801	12	28	theme	wall-membrane	1529:1541	arg1	complex					1543:1549	the cell wall-membrane complex	1520:1549	the cell wall-membrane complex	1520:1549	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	9	29	theme	H+	1251:1252	arg1	capacity					1261:1268	a correspondingly greater H+ efflux capacity	1225:1268	a correspondingly greater H+ efflux capacity	1225:1268	Root ATPase activity was slightly higher in the tolerant species with a correspondingly greater H+ efflux capacity.					
29524801	10	30	theme	boron-bridged	1342:1354	arg1	form					1364:1367	their boron-bridged dimeric form	1336:1367	their boron-bridged dimeric form	1336:1367	In both species, the great majority of the RG-II domains were in their boron-bridged dimeric form.					
29524801	13	31	theme	toxic	1719:1723	arg1	build-up					1728:1735	toxic H+ build-up	1719:1735	toxic H+ build-up	1719:1735	Thus, by maintaining root cell structure, active proton efflux avoided toxic H+ build-up in the cytoplasm and supported greater nutrient acquisition in H+-tolerant species.					
29524801	5	32	theme	cell-wall	736:744	arg1	polysaccharide					753:766	the cell-wall pectic polysaccharide	732:766	the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II)	732:803	Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.					
29524801	5	33	theme	polysaccharide	753:766	arg1	RG-II					798:802	RG-II	798:802	RG-II	798:802	Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.					
29524801	5	33	theme	polysaccharide	753:766	arg1	rhamnogalacturonan-II					775:795	the cell-wall pectic polysaccharide domain rhamnogalacturonan-II	732:795	the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II)	732:803	Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.					
29524801	9	34	theme	greater	1243:1249	arg1	capacity					1261:1268	a correspondingly greater H+ efflux capacity	1225:1268	a correspondingly greater H+ efflux capacity	1225:1268	Root ATPase activity was slightly higher in the tolerant species with a correspondingly greater H+ efflux capacity.					
29524801	13	35	theme	active	1690:1695	arg1	efflux					1704:1709	active proton efflux	1690:1709	active proton efflux	1690:1709	Thus, by maintaining root cell structure, active proton efflux avoided toxic H+ build-up in the cytoplasm and supported greater nutrient acquisition in H+-tolerant species.					
29524801	5	36	theme	ATPase	712:717	arg1	activity					719:726	Membrane ATPase activity	703:726	Membrane ATPase activity	703:726	Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.					
29524801	9	37	theme	efflux	1254:1259	arg1	capacity					1261:1268	a correspondingly greater H+ efflux capacity	1225:1268	a correspondingly greater H+ efflux capacity	1225:1268	Root ATPase activity was slightly higher in the tolerant species with a correspondingly greater H+ efflux capacity.					
29524801	0	38	theme	nutrient	22:29	arg1	retention					31:39	nutrient retention	22:39	nutrient retention	22:39	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	8	39	theme	low	1058:1060	arg1	pH					1062:1063	Very low pH	1053:1063	Very low pH (pH 2)	1053:1070	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	8	39	theme	low	1058:1060	arg1	pH					1066:1067	pH 2	1066:1069	pH 2	1066:1069	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	13	40	theme	nutrient	1776:1783	arg1	acquisition					1785:1795	greater nutrient acquisition	1768:1795	greater nutrient acquisition	1768:1795	Thus, by maintaining root cell structure, active proton efflux avoided toxic H+ build-up in the cytoplasm and supported greater nutrient acquisition in H+-tolerant species.					
29524801	8	41	from	loss	1095:1098	arg1	species					1146:1152	the sensitive species	1132:1152	the sensitive species	1132:1152	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	6	42	theme	RESULTS	858:864	arg1	Variation					866:874	RESULTS Variation	858:874	RESULTS Variation in survival, growth and mineral composition	858:918	RESULTS Variation in survival, growth and mineral composition was found between species.					
29524801	1	43	theme	particular	401:410	arg1	location					412:419	their particular location	395:419	their particular location	395:419	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	8	44	theme	significant	1083:1093	arg1	loss					1095:1098	a significant loss	1081:1098	a significant loss of root nutrients (K, P, Mg) in the sensitive species	1081:1152	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	12	45	theme	acid	1625:1628	arg1	tolerance					1637:1645	acid stress tolerance	1625:1645	acid stress tolerance	1625:1645	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	12	46	attach	linked	1510:1515	arg1	complex					1543:1549	the cell wall-membrane complex	1520:1549	the cell wall-membrane complex	1520:1549	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	12	46	attach	linked	1510:1515	arg2	elements					1501:1508	elements	1501:1508	elements linked to the cell wall-membrane complex	1501:1549	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	12	47	dep	components	1578:1587	arg1	H+-ATPases					1597:1606	H+-ATPases	1597:1606	H+-ATPases	1597:1606	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	12	47	dep	components	1578:1587	arg1	RG-II					1590:1594	RG-II	1590:1594	RG-II	1590:1594	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	1	48	theme	BACKGROUND	157:166	arg1	Tolerance					177:185	BACKGROUND AND AIMS Tolerance	157:185	BACKGROUND AND AIMS Tolerance to soil acidity	157:201	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	8	49	theme	nutrients	1108:1116	arg1	loss					1095:1098	a significant loss	1081:1098	a significant loss of root nutrients (K, P, Mg) in the sensitive species	1081:1152	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	8	50	theme	sensitive	1136:1144	arg1	species					1146:1152	the sensitive species	1132:1152	the sensitive species	1132:1152	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	7	51	theme	nutrients	1024:1032	arg1	concentration					1007:1019	greater concentration	999:1019	greater concentration of nutrients	999:1032	The H+-tolerant species (Erica andevalensis) showed greater concentration of nutrients than E. australis.					
29524801	13	52	theme	H+-tolerant	1800:1810	arg1	species					1812:1818	H+-tolerant species	1800:1818	H+-tolerant species	1800:1818	Thus, by maintaining root cell structure, active proton efflux avoided toxic H+ build-up in the cytoplasm and supported greater nutrient acquisition in H+-tolerant species.					
29524801	1	53	theme	AIMS	172:175	arg1	Tolerance					177:185	BACKGROUND AND AIMS Tolerance	157:185	BACKGROUND AND AIMS Tolerance to soil acidity	157:201	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	12	54	from	Variation	1488:1496	arg1	elements					1501:1508	elements	1501:1508	elements linked to the cell wall-membrane complex	1501:1549	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	2	55	theme	nutrient	469:476	arg1	solutions					478:486	nutrient solutions	469:486	nutrient solutions using seeds collected in SW Spain	469:520	METHODS Tolerance to H+ toxicity was tested in nutrient solutions using seeds collected in SW Spain.					
29524801	7	56	theme	Erica	972:976	arg1	species					963:969	The H+-tolerant species	947:969	The H+-tolerant species (Erica andevalensis)	947:990	The H+-tolerant species (Erica andevalensis) showed greater concentration of nutrients than E. australis.					
29524801	7	56	theme	Erica	972:976	arg1	andevalensis					978:989	Erica andevalensis	972:989	Erica andevalensis	972:989	The H+-tolerant species (Erica andevalensis) showed greater concentration of nutrients than E. australis.					
29524801	10	57	from	form	1364:1367	arg1	species					1279:1285	both species	1274:1285	both species	1274:1285	In both species, the great majority of the RG-II domains were in their boron-bridged dimeric form.					
29524801	0	58	theme	toxicity	113:120	arg1	tolerance					93:101	greater tolerance	85:101	greater tolerance of proton toxicity in the roots of two Erica species	85:154	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	10	59	theme	great	1292:1296	arg1	majority					1298:1305	the great majority	1288:1305	the great majority of the RG-II domains	1288:1326	In both species, the great majority of the RG-II domains were in their boron-bridged dimeric form.					
29524801	0	60	theme	Erica	142:146	arg1	species					148:154	two Erica species	138:154	two Erica species	138:154	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	1	61	theme	H+	356:357	arg1	tolerance					359:367	H+ tolerance	356:367	H+ tolerance	356:367	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	0	62	theme	pectin	63:68	arg1	efflux					14:19	Active proton efflux	0:19	Active proton efflux	0:19	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	0	62	theme	pectin	63:68	arg1	retention					31:39	nutrient retention	22:39	nutrient retention	22:39	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	0	62	theme	pectin	63:68	arg1	boron-bridging					45:58	boron-bridging	45:58	boron-bridging of pectin	45:68	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	3	63	theme	nutrient	540:547	arg1	contents					549:556	nutrient contents	540:556	nutrient contents	540:556	Plant growth and nutrient contents in leaves, stems and roots were determined.					
29524801	5	64	theme	domain	768:773	arg1	RG-II					798:802	RG-II	798:802	RG-II	798:802	Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.					
29524801	5	64	theme	domain	768:773	arg1	rhamnogalacturonan-II					775:795	the cell-wall pectic polysaccharide domain rhamnogalacturonan-II	732:795	the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II)	732:803	Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.					
29524801	12	65	theme	components	1578:1587	arg1	Variation					1488:1496	Variation	1488:1496	Variation in elements linked to the cell wall-membrane complex	1488:1549	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	12	65	theme	components	1578:1587	arg1	stability					1559:1567	the stability	1555:1567	the stability of their components (RG-II, H+-ATPases)	1555:1607	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	12	65	theme	components	1578:1587	arg1	CONCLUSIONS					1476:1486	CONCLUSIONS	1476:1486	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases)	1476:1607	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	13	66	theme	H+	1725:1726	arg1	build-up					1728:1735	toxic H+ build-up	1719:1735	toxic H+ build-up	1719:1735	Thus, by maintaining root cell structure, active proton efflux avoided toxic H+ build-up in the cytoplasm and supported greater nutrient acquisition in H+-tolerant species.					
29524801	5	67	theme	interspecific	831:843	arg1	differences					845:855	interspecific differences	831:855	interspecific differences	831:855	Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.					
29524801	2	68	theme	METHODS	422:428	arg1	Tolerance					430:438	METHODS Tolerance	422:438	METHODS Tolerance to H+ toxicity	422:453	METHODS Tolerance to H+ toxicity was tested in nutrient solutions using seeds collected in SW Spain.					
29524801	4	69	theme	proton	622:627	arg1	exchange					629:636	proton exchange	622:636	proton exchange	622:636	Viability tests and proton exchange were studied in roots exposed, short-term, to acidic conditions.					
29524801	0	70	theme	greater	85:91	arg1	tolerance					93:101	greater tolerance	85:101	greater tolerance of proton toxicity in the roots of two Erica species	85:154	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	10	71	theme	RG-II	1314:1318	arg1	domains					1320:1326	the RG-II domains	1310:1326	the RG-II domains	1310:1326	In both species, the great majority of the RG-II domains were in their boron-bridged dimeric form.					
29524801	13	72	theme	proton	1697:1702	arg1	efflux					1704:1709	active proton efflux	1690:1709	active proton efflux	1690:1709	Thus, by maintaining root cell structure, active proton efflux avoided toxic H+ build-up in the cytoplasm and supported greater nutrient acquisition in H+-tolerant species.					
29524801	9	73	theme	tolerant	1203:1210	arg1	species					1212:1218	the tolerant species	1199:1218	the tolerant species	1199:1218	Root ATPase activity was slightly higher in the tolerant species with a correspondingly greater H+ efflux capacity.					
29524801	10	74	theme	dimeric	1356:1362	arg1	form					1364:1367	their boron-bridged dimeric form	1336:1367	their boron-bridged dimeric form	1336:1367	In both species, the great majority of the RG-II domains were in their boron-bridged dimeric form.					
29524801	0	75	theme	proton	7:12	arg1	efflux					14:19	Active proton efflux	0:19	Active proton efflux	0:19	Active proton efflux, nutrient retention and boron-bridging of pectin are related to greater tolerance of proton toxicity in the roots of two Erica species.					
29524801	12	76	theme	cell	1524:1527	arg1	complex					1543:1549	the cell wall-membrane complex	1520:1549	the cell wall-membrane complex	1520:1549	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	4	77	theme	exposed	660:666	arg1	roots					654:658	roots	654:658	roots exposed, short-term	654:678	Viability tests and proton exchange were studied in roots exposed, short-term, to acidic conditions.					
29524801	13	78	theme	cell	1674:1677	arg1	structure					1679:1687	root cell structure	1669:1687	root cell structure	1669:1687	Thus, by maintaining root cell structure, active proton efflux avoided toxic H+ build-up in the cytoplasm and supported greater nutrient acquisition in H+-tolerant species.					
29524801	5	79	theme	pectic	746:751	arg1	polysaccharide					753:766	the cell-wall pectic polysaccharide	732:766	the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II)	732:803	Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.					
29524801	3	80	from	growth	529:534	arg1	roots					579:583	roots	579:583	roots	579:583	Plant growth and nutrient contents in leaves, stems and roots were determined.					
29524801	3	80	from	growth	529:534	arg1	stems					569:573	stems	569:573	stems	569:573	Plant growth and nutrient contents in leaves, stems and roots were determined.					
29524801	3	80	from	growth	529:534	arg1	leaves					561:566	leaves	561:566	leaves	561:566	Plant growth and nutrient contents in leaves, stems and roots were determined.					
29524801	5	81	theme	Membrane	703:710	arg1	activity					719:726	Membrane ATPase activity	703:726	Membrane ATPase activity	703:726	Membrane ATPase activity and the cell-wall pectic polysaccharide domain rhamnogalacturonan-II (RG-II) were analysed to find out interspecific differences.					
29524801	1	82	theme	mine-contaminated	256:272	arg1	Spain					296:300	Spain	296:300	Spain	296:300	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	1	82	theme	mine-contaminated	256:272	arg1	soils					274:278	mine-contaminated soils	256:278	mine-contaminated soils (S Portugal, SW Spain)	256:301	BACKGROUND AND AIMS Tolerance to soil acidity was studied in two species of Ericaceae that grow in mine-contaminated soils (S Portugal, SW Spain) to find out if there are interspecific variations in H+ tolerance which might be related to their particular location.					
29524801	8	83	dep	nutrients	1108:1116	arg1	K					1119:1119	K	1119:1119	K	1119:1119	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	8	83	dep	nutrients	1108:1116	arg1	Mg					1125:1126	Mg	1125:1126	Mg	1125:1126	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	8	83	dep	nutrients	1108:1116	arg1	P					1122:1122	P	1122:1122	P	1122:1122	Very low pH (pH 2) produced a significant loss of root nutrients (K, P, Mg) in the sensitive species.					
29524801	12	84	theme	stress	1630:1635	arg1	tolerance					1637:1645	acid stress tolerance	1625:1645	acid stress tolerance	1625:1645	CONCLUSIONS Variation in elements linked to the cell wall-membrane complex and the stability of their components (RG-II, H+-ATPases) are crucial for acid stress tolerance.					
29524801	9	85	theme	Root	1155:1158	arg1	activity					1167:1174	Root ATPase activity	1155:1174	Root ATPase activity	1155:1174	Root ATPase activity was slightly higher in the tolerant species with a correspondingly greater H+ efflux capacity.					
29524801	11	86	theme	sensitive	1457:1465	arg1	species					1467:1473	the sensitive species	1453:1473	the sensitive species	1453:1473	However, shifting to a medium of pH 2 caused some of the boron bridges to break in the sensitive species.					
29575932	8	0	theme	In	1287:1288	arg1	results					1313:1319	In vivo anti-inflammation results	1287:1319	In vivo anti-inflammation results	1287:1319	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	2	1	theme	morphogenetic	370:382	arg1	F127					331:334	Pluronic F127	322:334	Pluronic F127	322:334	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	2	1	theme	morphogenetic	370:382	arg1	BMP-2					393:397	BMP-2	393:397	BMP-2	393:397	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	2	1	theme	morphogenetic	370:382	arg1	protein					384:390	bone morphogenetic protein	365:390	bone morphogenetic protein (BMP-2)	365:398	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	8	2	theme	control	1484:1490	arg1	BMP-2					1506:1510	PF127@BMP-2 or BMP-2 saline solution	1500:1535	BMP-2	1506:1510	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	2	theme	control	1484:1490	arg1	solution					1528:1535	PF127@BMP-2 or BMP-2 saline solution	1500:1535	solution	1528:1535	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	2	theme	control	1484:1490	arg1	groups					1492:1497	the control groups	1480:1497	the control groups (PF127@BMP-2 or BMP-2 saline solution)	1480:1536	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	0	3	theme	cartilage	74:82	arg1	recovery					54:61	the recovery	50:61	the recovery of injured cartilage induced by osteoarthritis	50:108	ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis.					
29575932	3	4	contain	containing	528:537	arg1	hydrogels					518:526	composite hydrogels	508:526	composite hydrogels containing chondroitin sulfate	508:557	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	4	contain	containing	528:537	arg2	sulfate					551:557	chondroitin sulfate	539:557	chondroitin sulfate	539:557	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	1	5	theme	promising	173:181	arg1	strategy					183:190	a promising strategy	171:190	a promising strategy for recovery of injured articular cartilage	171:234	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	1	5	theme	promising	173:181	arg1	injection					127:135	Intra-articular injection	111:135	Intra-articular injection of anti-inflammatory drugs	111:162	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	8	6	theme	BMP-2	1340:1344	arg1	hydrogels					1356:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	7	theme	@	1505:1505	arg1	BMP-2					1506:1510	PF127@BMP-2 or BMP-2 saline solution	1500:1535	BMP-2	1506:1510	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	7	theme	@	1505:1505	arg1	groups					1492:1497	the control groups	1480:1497	the control groups (PF127@BMP-2 or BMP-2 saline solution)	1480:1536	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	2	8	theme	bone	365:368	arg1	F127					331:334	Pluronic F127	322:334	Pluronic F127	322:334	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	2	8	theme	bone	365:368	arg1	BMP-2					393:397	BMP-2	393:397	BMP-2	393:397	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	2	8	theme	bone	365:368	arg1	protein					384:390	bone morphogenetic protein	365:390	bone morphogenetic protein (BMP-2)	365:398	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	7	9	theme	cell	1272:1275	arg1	MC3T3-E1					1277:1284	mouse pre-osteoblast cell MC3T3-E1	1251:1284	mouse pre-osteoblast cell MC3T3-E1	1251:1284	Subsequently, MTT experiments were performed to investigate the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1.					
29575932	2	10	theme	Pluronic	322:329	arg1	F127					331:334	Pluronic F127	322:334	Pluronic F127	322:334	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	2	10	theme	Pluronic	322:329	arg1	protein					384:390	bone morphogenetic protein	365:390	bone morphogenetic protein (BMP-2)	365:398	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	2	10	theme	Pluronic	322:329	arg1	glycosaminoglycan					337:353	glycosaminoglycan	337:353	glycosaminoglycan (GAG)	337:359	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	5	11	theme	BSA	902:904	arg1	16 days					870:876	16 days	870:876	16 days	870:876	The results showed that hydrogels could maintain shapes for more than 16 days and the release rate of BSA in PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG.					
29575932	5	11	theme	BSA	902:904	arg1	rate					894:897	the release rate	882:897	the release rate of BSA	882:904	The results showed that hydrogels could maintain shapes for more than 16 days and the release rate of BSA in PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG.					
29575932	6	12	theme	dissolution	1107:1117	arg1	rate					1119:1122	hydrogel dissolution rate	1098:1122	hydrogel dissolution rate	1098:1122	Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels.					
29575932	8	13	theme	anti-inflammation	1295:1311	arg1	results					1313:1319	In vivo anti-inflammation results	1287:1319	In vivo anti-inflammation results	1287:1319	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	4	14	theme	PF/GAG	731:736	arg1	hydrogels					738:746	PF/GAG hydrogels	731:746	PF/GAG hydrogels	731:746	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	3	15	with	properties	473:482	arg1	mass					603:606	different hyaluronic acid molecular mass	567:606	different hyaluronic acid molecular mass (10k, 90k, 800k)	567:623	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	16	theme	rheological	461:471	arg1	properties					473:482	The rheological properties	457:482	The rheological properties	457:482	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	17	theme	chondroitin	539:549	arg1	sulfate					551:557	chondroitin sulfate	539:557	chondroitin sulfate	539:557	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	18	dep	mass	603:606	arg1	10k					609:611	10k	609:611	10k	609:611	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	18	dep	mass	603:606	arg1	800k					619:622	800k	619:622	800k	619:622	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	18	dep	mass	603:606	arg1	90k					614:616	90k	614:616	90k	614:616	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	7	19	from	toxicity	1222:1229	arg1	MC3T3-E1					1277:1284	mouse pre-osteoblast cell MC3T3-E1	1251:1284	mouse pre-osteoblast cell MC3T3-E1	1251:1284	Subsequently, MTT experiments were performed to investigate the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1.					
29575932	2	20	theme	hydrogels	299:307	arg1	series					251:256	a series	249:256	a series	249:256	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	6	21	theme	increasing	1021:1030	arg1	weight					1046:1051	the molecular weight	1032:1051	increasing the molecular weight of hyaluronic acid	1021:1070	Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels.					
29575932	7	22	theme	MTT	1172:1174	arg1	experiments					1176:1186	MTT experiments	1172:1186	MTT experiments	1172:1186	Subsequently, MTT experiments were performed to investigate the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1.					
29575932	5	23	theme	PF127	955:959	arg1	gels					961:964	PF127 gels	955:964	PF127 gels	955:964	The results showed that hydrogels could maintain shapes for more than 16 days and the release rate of BSA in PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG.					
29575932	3	24	theme	acid	588:591	arg1	mass					603:606	different hyaluronic acid molecular mass	567:606	different hyaluronic acid molecular mass (10k, 90k, 800k)	567:623	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	25	theme	hyaluronic	577:586	arg1	acid					588:591	different hyaluronic acid	567:591	different hyaluronic acid molecular mass (10k, 90k, 800k)	567:623	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	26	with	rate	500:503	arg1	mass					603:606	different hyaluronic acid molecular mass	567:606	different hyaluronic acid molecular mass (10k, 90k, 800k)	567:623	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	27	theme	molecular	593:601	arg1	mass					603:606	different hyaluronic acid molecular mass	567:606	different hyaluronic acid molecular mass (10k, 90k, 800k)	567:623	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	8	28	theme	efficient	1379:1387	arg1	efficacy					1389:1396	the most efficient efficacy	1370:1396	the most efficient efficacy	1370:1396	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	4	29	theme	serum	662:666	arg1	Meanwhile					644:652	Meanwhile	644:652	Meanwhile	644:652	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	29	theme	serum	662:666	arg1	drug					714:717	model drug	708:717	model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro	708:797	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	29	theme	serum	662:666	arg1	BSA					677:679	BSA	677:679	BSA	677:679	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	29	theme	serum	662:666	arg1	FITC-BSA					685:692	FITC-BSA	685:692	FITC-BSA	685:692	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	29	theme	serum	662:666	arg1	albumin					668:674	bovine serum albumin	655:674	bovine serum albumin (BSA)	655:680	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	0	30	theme	thermo-sensitive	21:36	arg1	hydrogel					38:45	injectable thermo-sensitive hydrogel	10:45	injectable thermo-sensitive hydrogel	10:45	ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis.					
29575932	4	31	theme	model	708:712	arg1	Meanwhile					644:652	Meanwhile	644:652	Meanwhile	644:652	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	31	theme	model	708:712	arg1	drug					714:717	model drug	708:717	model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro	708:797	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	31	theme	model	708:712	arg1	FITC-BSA					685:692	FITC-BSA	685:692	FITC-BSA	685:692	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	31	theme	model	708:712	arg1	albumin					668:674	bovine serum albumin	655:674	bovine serum albumin (BSA)	655:680	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	3	32	theme	different	567:575	arg1	acid					588:591	different hyaluronic acid	567:591	different hyaluronic acid molecular mass (10k, 90k, 800k)	567:623	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	5	33	theme	composite	916:924	arg1	gels					926:929	PF/GAG composite gels	909:929	PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG	909:1005	The results showed that hydrogels could maintain shapes for more than 16 days and the release rate of BSA in PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG.					
29575932	1	34	theme	injured	208:214	arg1	cartilage					226:234	injured articular cartilage	208:234	injured articular cartilage	208:234	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	2	35	theme	extracellular	429:441	arg1	ECM					451:453	ECM	451:453	ECM	451:453	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	2	35	theme	extracellular	429:441	arg1	matrix					443:448	extracellular matrix	429:448	extracellular matrix (ECM)	429:454	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	6	36	theme	BSA	1128:1130	arg1	release					1132:1138	BSA release	1128:1138	BSA release	1128:1138	Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels.					
29575932	8	37	theme	injured	1413:1419	arg1	cartilage					1421:1429	injured cartilage	1413:1429	injured cartilage	1413:1429	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	1	38	theme	Intra-articular	111:125	arg1	injection					127:135	Intra-articular injection	111:135	Intra-articular injection of anti-inflammatory drugs	111:162	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	1	38	theme	Intra-articular	111:125	arg1	strategy					183:190	a promising strategy	171:190	a promising strategy for recovery of injured articular cartilage	171:234	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	1	39	theme	articular	216:224	arg1	cartilage					226:234	injured articular cartilage	208:234	injured articular cartilage	208:234	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	6	40	theme	acid	1067:1070	arg1	weight					1046:1051	the molecular weight	1032:1051	increasing the molecular weight of hyaluronic acid	1021:1070	Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels.					
29575932	1	41	theme	cartilage	226:234	arg1	recovery					196:203	recovery	196:203	recovery of injured articular cartilage	196:234	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	4	42	theme	bovine	655:660	arg1	Meanwhile					644:652	Meanwhile	644:652	Meanwhile	644:652	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	42	theme	bovine	655:660	arg1	drug					714:717	model drug	708:717	model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro	708:797	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	42	theme	bovine	655:660	arg1	BSA					677:679	BSA	677:679	BSA	677:679	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	42	theme	bovine	655:660	arg1	FITC-BSA					685:692	FITC-BSA	685:692	FITC-BSA	685:692	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	42	theme	bovine	655:660	arg1	albumin					668:674	bovine serum albumin	655:674	bovine serum albumin (BSA)	655:680	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	8	43	theme	saline	1521:1526	arg1	groups					1492:1497	the control groups	1480:1497	the control groups (PF127@BMP-2 or BMP-2 saline solution)	1480:1536	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	43	theme	saline	1521:1526	arg1	solution					1528:1535	PF127@BMP-2 or BMP-2 saline solution	1500:1535	solution	1528:1535	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	44	dep	In	1287:1288	arg1	vivo					1290:1293	vivo	1290:1293	vivo	1290:1293	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	7	45	theme	mouse	1251:1255	arg1	MC3T3-E1					1277:1284	mouse pre-osteoblast cell MC3T3-E1	1251:1284	mouse pre-osteoblast cell MC3T3-E1	1251:1284	Subsequently, MTT experiments were performed to investigate the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1.					
29575932	8	46	theme	BMP-2	1515:1519	arg1	groups					1492:1497	the control groups	1480:1497	the control groups (PF127@BMP-2 or BMP-2 saline solution)	1480:1536	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	46	theme	BMP-2	1515:1519	arg1	solution					1528:1535	PF127@BMP-2 or BMP-2 saline solution	1500:1535	solution	1528:1535	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	47	theme	@	1339:1339	arg1	hydrogels					1356:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	48	theme	cartilage	1421:1429	arg1	recovery					1401:1408	recovery	1401:1408	recovery	1401:1408	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	49	theme	PF/GAG	1333:1338	arg1	hydrogels					1356:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	3	50	theme	dissolution	488:498	arg1	rate					500:503	dissolution rate	488:503	dissolution rate	488:503	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	8	51	contain	had	1366:1368	arg1	hydrogels					1356:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	51	contain	had	1366:1368	arg2	efficacy					1389:1396	the most efficient efficacy	1370:1396	the most efficient efficacy	1370:1396	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	6	52	theme	hydrogel	1098:1105	arg1	rate					1119:1122	hydrogel dissolution rate	1098:1122	hydrogel dissolution rate	1098:1122	Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels.					
29575932	3	53	theme	composite	508:516	arg1	hydrogels					518:526	composite hydrogels	508:526	composite hydrogels containing chondroitin sulfate	508:557	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	5	54	theme	release	886:892	arg1	rate					894:897	the release rate	882:897	the release rate of BSA	882:904	The results showed that hydrogels could maintain shapes for more than 16 days and the release rate of BSA in PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG.					
29575932	4	55	theme	release	773:779	arg1	behavior					781:788	their sustained release behavior	757:788	their sustained release behavior	757:788	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	4	56	theme	sustained	763:771	arg1	behavior					781:788	their sustained release behavior	757:788	their sustained release behavior	757:788	Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro.					
29575932	2	57	theme	composite	289:297	arg1	hydrogels					299:307	injectable thermo-sensitive composite hydrogels	261:307	injectable thermo-sensitive composite hydrogels	261:307	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	1	58	theme	anti-inflammatory	140:156	arg1	drugs					158:162	anti-inflammatory drugs	140:162	anti-inflammatory drugs	140:162	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	5	59	theme	PF/GAG	909:914	arg1	gels					926:929	PF/GAG composite gels	909:929	PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG	909:1005	The results showed that hydrogels could maintain shapes for more than 16 days and the release rate of BSA in PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG.					
29575932	7	60	theme	hydrogels	1238:1246	arg1	toxicity					1222:1229	the toxicity	1218:1229	the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1	1218:1284	Subsequently, MTT experiments were performed to investigate the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1.					
29575932	2	61	theme	thermo-sensitive	272:287	arg1	hydrogels					299:307	injectable thermo-sensitive composite hydrogels	261:307	injectable thermo-sensitive composite hydrogels	261:307	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	1	62	theme	drugs	158:162	arg1	injection					127:135	Intra-articular injection	111:135	Intra-articular injection of anti-inflammatory drugs	111:162	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	1	62	theme	drugs	158:162	arg1	strategy					183:190	a promising strategy	171:190	a promising strategy for recovery of injured articular cartilage	171:234	Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage.					
29575932	6	63	theme	hyaluronic	1056:1065	arg1	acid					1067:1070	hyaluronic acid	1056:1070	hyaluronic acid	1056:1070	Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels.					
29575932	0	64	theme	injured	66:72	arg1	cartilage					74:82	injured cartilage	66:82	injured cartilage	66:82	ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis.					
29575932	8	65	theme	composite	1346:1354	arg1	hydrogels					1356:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	PF/GAG@BMP-2 composite hydrogels	1333:1364	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	2	66	theme	injectable	261:270	arg1	hydrogels					299:307	injectable thermo-sensitive composite hydrogels	261:307	injectable thermo-sensitive composite hydrogels	261:307	We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM).					
29575932	7	67	theme	pre-osteoblast	1257:1270	arg1	MC3T3-E1					1277:1284	mouse pre-osteoblast cell MC3T3-E1	1251:1284	mouse pre-osteoblast cell MC3T3-E1	1251:1284	Subsequently, MTT experiments were performed to investigate the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1.					
29575932	3	68	theme	hydrogels	518:526	arg1	properties					473:482	The rheological properties	457:482	The rheological properties	457:482	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	3	68	theme	hydrogels	518:526	arg1	rate					500:503	dissolution rate	488:503	dissolution rate	488:503	The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated.					
29575932	0	69	theme	injectable	10:19	arg1	hydrogel					38:45	injectable thermo-sensitive hydrogel	10:45	injectable thermo-sensitive hydrogel	10:45	ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis.					
29575932	6	70	theme	molecular	1036:1044	arg1	weight					1046:1051	the molecular weight	1032:1051	increasing the molecular weight of hyaluronic acid	1021:1070	Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels.					
29575932	8	71	dep	groups	1492:1497	arg1	BMP-2					1506:1510	PF127@BMP-2 or BMP-2 saline solution	1500:1535	BMP-2	1506:1510	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	71	dep	groups	1492:1497	arg1	groups					1492:1497	the control groups	1480:1497	the control groups (PF127@BMP-2 or BMP-2 saline solution)	1480:1536	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
29575932	8	71	dep	groups	1492:1497	arg1	solution					1528:1535	PF127@BMP-2 or BMP-2 saline solution	1500:1535	solution	1528:1535	In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).					
31173829	7	0	theme	nanocomposite	931:943	arg1	films					945:949	The developed nanocomposite films	917:949	The developed nanocomposite films	917:949	The developed nanocomposite films were demonstrated to have enhanced water barrier, mechanical and antimicrobial properties.					
31173829	1	1	theme	ready	169:173	arg1	food					182:185	ready to eat food	169:185	ready to eat food	169:185	The increasing acceptance of ready to eat food generates demand on development of active and intelligent food packaging material.					
31173829	5	2	theme	oxide	761:765	arg1	nanoparticles					767:779	zinc oxide nanoparticles	756:779	zinc oxide nanoparticles	756:779	In the present study, starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals were prepared by solvent casting technique.					
31173829	1	3	theme	material	260:267	arg1	development					207:217	development	207:217	development of active and intelligent food packaging material	207:267	The increasing acceptance of ready to eat food generates demand on development of active and intelligent food packaging material.					
31173829	0	4	theme	pH	91:92	arg1	sensing					94:100	intelligent pH sensing	79:100	intelligent pH sensing	79:100	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing wraps for food packaging application.					
31173829	2	5	contain	have	335:338	arg2	limitations					340:350	limitations	340:350	limitations for broad applications	340:373	Even though many polymers have been used for the packaging, they have limitations for broad applications.					
31173829	2	5	contain	have	335:338	arg1	they					330:333	they	330:333	they	330:333	Even though many polymers have been used for the packaging, they have limitations for broad applications.					
31173829	8	6	theme	food	1173:1176	arg1	applications					1188:1199	food packaging applications	1173:1199	food packaging applications	1173:1199	The unique features of the nanocomposite with its pH indication property demonstrated in the study indicate its potential usage in food packaging applications.					
31173829	4	7	theme	mechanical	601:610	arg1	properties					630:639	the mechanical and antimicrobial properties	597:639	the mechanical and antimicrobial properties of PVA	597:646	A variety of nano-fabrication techniques have already been reported to improve the mechanical and antimicrobial properties of PVA to exploit its wider applications.					
31173829	5	8	theme	based	716:720	arg1	films					732:736	starch-PVA based composite films	705:736	starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals	705:798	In the present study, starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals were prepared by solvent casting technique.					
31173829	8	9	theme	nanocomposite	1069:1081	arg1	features					1053:1060	The unique features	1042:1060	The unique features of the nanocomposite with its pH indication property demonstrated in the study	1042:1139	The unique features of the nanocomposite with its pH indication property demonstrated in the study indicate its potential usage in food packaging applications.					
31173829	7	10	theme	enhanced	977:984	arg1	barrier					992:998	enhanced water barrier	977:998	enhanced water barrier	977:998	The developed nanocomposite films were demonstrated to have enhanced water barrier, mechanical and antimicrobial properties.					
31173829	2	11	theme	broad	356:360	arg1	applications					362:373	broad applications	356:373	broad applications	356:373	Even though many polymers have been used for the packaging, they have limitations for broad applications.					
31173829	0	12	theme	packaging	117:125	arg1	application					127:137	food packaging application	112:137	food packaging application	112:137	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing wraps for food packaging application.					
31173829	8	13	theme	pH	1092:1093	arg1	property					1106:1113	its pH indication property	1088:1113	its pH indication property	1088:1113	The unique features of the nanocomposite with its pH indication property demonstrated in the study indicate its potential usage in food packaging applications.					
31173829	7	14	theme	water	986:990	arg1	barrier					992:998	enhanced water barrier	977:998	enhanced water barrier	977:998	The developed nanocomposite films were demonstrated to have enhanced water barrier, mechanical and antimicrobial properties.					
31173829	3	15	theme	promising	428:436	arg1	polymer					451:457	a promising film forming polymer	426:457	a promising film forming polymer with highly flexible, emulsifying and adhesive properties	426:515	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	3	15	theme	promising	428:436	arg1	Alcohol					415:421	Poly Vinyl Alcohol	404:421	Poly Vinyl Alcohol	404:421	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	0	16	theme	food	112:115	arg1	application					127:137	food packaging application	112:137	food packaging application	112:137	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing wraps for food packaging application.					
31173829	8	17	from	usage	1164:1168	arg1	applications					1188:1199	food packaging applications	1173:1199	food packaging applications	1173:1199	The unique features of the nanocomposite with its pH indication property demonstrated in the study indicate its potential usage in food packaging applications.					
31173829	2	18	theme	many	282:285	arg1	polymers					287:294	many polymers	282:294	many polymers	282:294	Even though many polymers have been used for the packaging, they have limitations for broad applications.					
31173829	1	19	theme	food	182:185	arg1	acceptance					155:164	The increasing acceptance	140:164	The increasing acceptance of ready to eat food	140:185	The increasing acceptance of ready to eat food generates demand on development of active and intelligent food packaging material.					
31173829	0	20	theme	composite	11:19	arg1	films					21:25	Starch-PVA composite films	0:25	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing	0:100	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing wraps for food packaging application.					
31173829	8	21	theme	packaging	1178:1186	arg1	applications					1188:1199	food packaging applications	1173:1199	food packaging applications	1173:1199	The unique features of the nanocomposite with its pH indication property demonstrated in the study indicate its potential usage in food packaging applications.					
31173829	4	22	theme	wider	663:667	arg1	applications					669:680	its wider applications	659:680	its wider applications	659:680	A variety of nano-fabrication techniques have already been reported to improve the mechanical and antimicrobial properties of PVA to exploit its wider applications.					
31173829	0	23	theme	Starch-PVA	0:9	arg1	films					21:25	Starch-PVA composite films	0:25	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing	0:100	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing wraps for food packaging application.					
31173829	8	24	theme	indication	1095:1104	arg1	property					1106:1113	its pH indication property	1088:1113	its pH indication property	1088:1113	The unique features of the nanocomposite with its pH indication property demonstrated in the study indicate its potential usage in food packaging applications.					
31173829	7	25	contain	have	972:975	arg2	barrier					992:998	enhanced water barrier	977:998	enhanced water barrier	977:998	The developed nanocomposite films were demonstrated to have enhanced water barrier, mechanical and antimicrobial properties.					
31173829	7	25	contain	have	972:975	arg2	properties					1030:1039	mechanical and antimicrobial properties	1001:1039	mechanical and antimicrobial properties	1001:1039	The developed nanocomposite films were demonstrated to have enhanced water barrier, mechanical and antimicrobial properties.					
31173829	7	25	contain	have	972:975	arg1	films					945:949	The developed nanocomposite films	917:949	The developed nanocomposite films	917:949	The developed nanocomposite films were demonstrated to have enhanced water barrier, mechanical and antimicrobial properties.					
31173829	5	26	theme	starch-PVA	705:714	arg1	films					732:736	starch-PVA based composite films	705:736	starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals	705:798	In the present study, starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals were prepared by solvent casting technique.					
31173829	8	27	theme	unique	1046:1051	arg1	features					1053:1060	The unique features	1042:1060	The unique features of the nanocomposite with its pH indication property demonstrated in the study	1042:1139	The unique features of the nanocomposite with its pH indication property demonstrated in the study indicate its potential usage in food packaging applications.					
31173829	8	28	with	features	1053:1060	arg1	property					1106:1113	its pH indication property	1088:1113	its pH indication property	1088:1113	The unique features of the nanocomposite with its pH indication property demonstrated in the study indicate its potential usage in food packaging applications.					
31173829	3	29	with	polymer	451:457	arg1	properties					506:515	highly flexible, emulsifying and adhesive properties	464:515	highly flexible, emulsifying and adhesive properties	464:515	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	6	30	theme	UV-Vis	888:893	arg1	spectrometry					895:906	UV-Vis spectrometry	888:906	UV-Vis spectrometry	888:906	The films were characterized by XRD, FT-IR, UV-Vis spectrometry and SEM.					
31173829	4	31	theme	PVA	644:646	arg1	properties					630:639	the mechanical and antimicrobial properties	597:639	the mechanical and antimicrobial properties of PVA	597:646	A variety of nano-fabrication techniques have already been reported to improve the mechanical and antimicrobial properties of PVA to exploit its wider applications.					
31173829	3	32	theme	Vinyl	409:413	arg1	polymer					451:457	a promising film forming polymer	426:457	a promising film forming polymer with highly flexible, emulsifying and adhesive properties	426:515	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	3	32	theme	Vinyl	409:413	arg1	Alcohol					415:421	Poly Vinyl Alcohol	404:421	Poly Vinyl Alcohol	404:421	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	5	33	theme	composite	722:730	arg1	films					732:736	starch-PVA based composite films	705:736	starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals	705:798	In the present study, starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals were prepared by solvent casting technique.					
31173829	7	34	theme	developed	921:929	arg1	films					945:949	The developed nanocomposite films	917:949	The developed nanocomposite films	917:949	The developed nanocomposite films were demonstrated to have enhanced water barrier, mechanical and antimicrobial properties.					
31173829	3	35	theme	forming	443:449	arg1	polymer					451:457	a promising film forming polymer	426:457	a promising film forming polymer with highly flexible, emulsifying and adhesive properties	426:515	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	3	35	theme	forming	443:449	arg1	Alcohol					415:421	Poly Vinyl Alcohol	404:421	Poly Vinyl Alcohol	404:421	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	0	36	theme	zinc-oxide	32:41	arg1	nanoparticles					43:55	zinc-oxide nanoparticles	32:55	zinc-oxide nanoparticles	32:55	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing wraps for food packaging application.					
31173829	1	37	theme	active	222:227	arg1	material					260:267	active and intelligent food packaging material	222:267	active and intelligent food packaging material	222:267	The increasing acceptance of ready to eat food generates demand on development of active and intelligent food packaging material.					
31173829	5	38	theme	solvent	817:823	arg1	technique					833:841	solvent casting technique	817:841	solvent casting technique	817:841	In the present study, starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals were prepared by solvent casting technique.					
31173829	3	39	theme	adhesive	497:504	arg1	properties					506:515	highly flexible, emulsifying and adhesive properties	464:515	highly flexible, emulsifying and adhesive properties	464:515	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	5	40	theme	casting	825:831	arg1	technique					833:841	solvent casting technique	817:841	solvent casting technique	817:841	In the present study, starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals were prepared by solvent casting technique.					
31173829	0	41	with	films	21:25	arg1	nanoparticles					43:55	zinc-oxide nanoparticles	32:55	zinc-oxide nanoparticles	32:55	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing wraps for food packaging application.					
31173829	0	41	with	films	21:25	arg1	phytochemicals					61:74	phytochemicals	61:74	phytochemicals	61:74	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing wraps for food packaging application.					
31173829	3	42	theme	emulsifying	481:491	arg1	properties					506:515	highly flexible, emulsifying and adhesive properties	464:515	highly flexible, emulsifying and adhesive properties	464:515	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	3	43	theme	Poly	404:407	arg1	polymer					451:457	a promising film forming polymer	426:457	a promising film forming polymer with highly flexible, emulsifying and adhesive properties	426:515	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	3	43	theme	Poly	404:407	arg1	Alcohol					415:421	Poly Vinyl Alcohol	404:421	Poly Vinyl Alcohol	404:421	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	4	44	theme	techniques	548:557	arg1	variety					520:526	A variety	518:526	A variety of nano-fabrication techniques	518:557	A variety of nano-fabrication techniques have already been reported to improve the mechanical and antimicrobial properties of PVA to exploit its wider applications.					
31173829	4	44	theme	techniques	548:557	arg1	techniques					548:557	nano-fabrication techniques	531:557	nano-fabrication techniques	531:557	A variety of nano-fabrication techniques have already been reported to improve the mechanical and antimicrobial properties of PVA to exploit its wider applications.					
31173829	5	45	theme	present	690:696	arg1	study					698:702	the present study	686:702	the present study	686:702	In the present study, starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals were prepared by solvent casting technique.					
31173829	2	46	used	used	306:309	arg2	polymers					287:294	many polymers	282:294	many polymers	282:294	Even though many polymers have been used for the packaging, they have limitations for broad applications.					
31173829	4	47	theme	nano-fabrication	531:546	arg1	techniques					548:557	nano-fabrication techniques	531:557	nano-fabrication techniques	531:557	A variety of nano-fabrication techniques have already been reported to improve the mechanical and antimicrobial properties of PVA to exploit its wider applications.					
31173829	3	48	theme	flexible	471:478	arg1	properties					506:515	highly flexible, emulsifying and adhesive properties	464:515	highly flexible, emulsifying and adhesive properties	464:515	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	4	49	theme	antimicrobial	616:628	arg1	properties					630:639	the mechanical and antimicrobial properties	597:639	the mechanical and antimicrobial properties of PVA	597:646	A variety of nano-fabrication techniques have already been reported to improve the mechanical and antimicrobial properties of PVA to exploit its wider applications.					
31173829	8	50	theme	potential	1154:1162	arg1	usage					1164:1168	its potential usage	1150:1168	its potential usage in food packaging applications	1150:1199	The unique features of the nanocomposite with its pH indication property demonstrated in the study indicate its potential usage in food packaging applications.					
31173829	7	51	theme	antimicrobial	1016:1028	arg1	properties					1030:1039	mechanical and antimicrobial properties	1001:1039	mechanical and antimicrobial properties	1001:1039	The developed nanocomposite films were demonstrated to have enhanced water barrier, mechanical and antimicrobial properties.					
31173829	1	52	theme	increasing	144:153	arg1	acceptance					155:164	The increasing acceptance	140:164	The increasing acceptance of ready to eat food	140:185	The increasing acceptance of ready to eat food generates demand on development of active and intelligent food packaging material.					
31173829	1	53	theme	intelligent	233:243	arg1	material					260:267	active and intelligent food packaging material	222:267	active and intelligent food packaging material	222:267	The increasing acceptance of ready to eat food generates demand on development of active and intelligent food packaging material.					
31173829	0	54	theme	intelligent	79:89	arg1	sensing					94:100	intelligent pH sensing	79:100	intelligent pH sensing	79:100	Starch-PVA composite films with zinc-oxide nanoparticles and phytochemicals as intelligent pH sensing wraps for food packaging application.					
31173829	5	55	theme	zinc	756:759	arg1	nanoparticles					767:779	zinc oxide nanoparticles	756:779	zinc oxide nanoparticles	756:779	In the present study, starch-PVA based composite films incorporated with zinc oxide nanoparticles and phytochemicals were prepared by solvent casting technique.					
31173829	3	56	theme	film	438:441	arg1	polymer					451:457	a promising film forming polymer	426:457	a promising film forming polymer with highly flexible, emulsifying and adhesive properties	426:515	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	3	56	theme	film	438:441	arg1	Alcohol					415:421	Poly Vinyl Alcohol	404:421	Poly Vinyl Alcohol	404:421	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	1	57	theme	food	245:248	arg1	material					260:267	active and intelligent food packaging material	222:267	active and intelligent food packaging material	222:267	The increasing acceptance of ready to eat food generates demand on development of active and intelligent food packaging material.					
31173829	7	58	theme	mechanical	1001:1010	arg1	properties					1030:1039	mechanical and antimicrobial properties	1001:1039	mechanical and antimicrobial properties	1001:1039	The developed nanocomposite films were demonstrated to have enhanced water barrier, mechanical and antimicrobial properties.					
31173829	3	59	theme	various	386:392	arg1	polymers					394:401	the various polymers	382:401	the various polymers	382:401	Among the various polymers, Poly Vinyl Alcohol is a promising film forming polymer with highly flexible, emulsifying and adhesive properties.					
31173829	1	60	theme	packaging	250:258	arg1	material					260:267	active and intelligent food packaging material	222:267	active and intelligent food packaging material	222:267	The increasing acceptance of ready to eat food generates demand on development of active and intelligent food packaging material.					
31739038	0	0	theme	films	82:86	arg1	properties					54:63	the structural, physical and functional properties	14:63	the structural, physical and functional properties of κ-carrageenan films	14:86	Comparison of the structural, physical and functional properties of κ-carrageenan films incorporated with pomegranate flesh and peel extracts.					
31739038	7	1	theme	pH-sensitive	1425:1436	arg1	property					1438:1445	pH-sensitive property	1425:1445	pH-sensitive property	1425:1445	Nonetheless, κ-carrageenan-PFE films exhibited pH-sensitive property because of abundant anthocyanins in PFE.					
31739038	6	2	theme	incorporation	1356:1368	arg1	levels					1370:1375	the same extract incorporation levels	1339:1375	the same extract incorporation levels	1339:1375	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	0	3	theme	κ-carrageenan	68:80	arg1	films					82:86	κ-carrageenan films	68:86	κ-carrageenan films	68:86	Comparison of the structural, physical and functional properties of κ-carrageenan films incorporated with pomegranate flesh and peel extracts.					
31739038	2	4	theme	structural	341:350	arg1	properties					407:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties	337:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films	337:465	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were compared for the first time.					
31739038	6	5	theme	κ-carrageenan-PPE	1207:1223	arg1	films					1225:1229	κ-carrageenan-PPE films	1207:1229	κ-carrageenan-PPE films	1207:1229	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	9	6	theme	food	1811:1814	arg1	industry					1816:1823	food industry	1811:1823	food industry	1811:1823	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	8	7	theme	extract	1624:1630	arg1	content					1613:1619	the content	1609:1619	the content of extract	1609:1630	Notably, the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were closely related to the content of extract.					
31739038	5	8	theme	tensile	1018:1024	arg1	strength					1026:1033	The tensile strength	1014:1033	The tensile strength	1014:1033	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	4	9	theme	malvidin-3-O-	831:843	arg1	6-acetyl					845:852	6-acetyl	845:852	6-acetyl	845:852	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	4	9	theme	malvidin-3-O-	831:843	arg1	-glucoside					854:863	malvidin-3-O-(6-acetyl)-glucoside	831:863	malvidin-3-O-(6-acetyl)-glucoside (4.07%)	831:871	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	4	9	theme	malvidin-3-O-	831:843	arg1	%					870:870	4.07%	866:870	4.07%	866:870	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	8	10	theme	films	1579:1583	arg1	properties					1525:1534	the physical and functional properties	1497:1534	the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films	1497:1583	Notably, the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were closely related to the content of extract.					
31739038	8	10	theme	films	1579:1583	arg1	related					1598:1604	related	1598:1604	related	1598:1604	Notably, the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were closely related to the content of extract.					
31739038	5	11	dep	strength	1026:1033	arg1	ability					1069:1075	barrier ability	1061:1075	barrier ability	1061:1075	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	8	12	theme	κ-carrageenan-PPE	1561:1577	arg1	films					1579:1583	κ-carrageenan-PFE and κ-carrageenan-PPE films	1539:1583	κ-carrageenan-PFE and κ-carrageenan-PPE films	1539:1583	Notably, the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were closely related to the content of extract.					
31739038	5	13	theme	film	1094:1097	arg1	light					1055:1059	UV light	1052:1059	UV light	1052:1059	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	5	13	theme	film	1094:1097	arg1	strength					1026:1033	The tensile strength	1014:1033	The tensile strength	1014:1033	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	5	13	theme	film	1094:1097	arg1	vapor					1042:1046	water vapor	1036:1046	water vapor	1036:1046	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	5	14	theme	water	1036:1040	arg1	vapor					1042:1046	water vapor	1036:1046	water vapor	1036:1046	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	1	15	theme	active	158:163	arg1	films					191:195	active and intelligent packaging films	158:195	active and intelligent packaging films	158:195	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	0	16	theme	pomegranate	106:116	arg1	flesh					118:122	pomegranate flesh and peel extracts	106:140	flesh	118:122	Comparison of the structural, physical and functional properties of κ-carrageenan films incorporated with pomegranate flesh and peel extracts.					
31739038	3	17	theme	6″-ethylmalonyl	674:688	arg1	-glucoside					690:699	pelargonidin-3-O- (6″-ethylmalonyl)-glucoside	655:699	pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%)	655:707	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	17	theme	6″-ethylmalonyl	674:688	arg1	%					706:706	7.05%	702:706	7.05%	702:706	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	0	18	theme	physical	30:37	arg1	properties					54:63	the structural, physical and functional properties	14:63	the structural, physical and functional properties of κ-carrageenan films	14:86	Comparison of the structural, physical and functional properties of κ-carrageenan films incorporated with pomegranate flesh and peel extracts.					
31739038	3	19	dep	showed	509:514	arg1	composed					524:531	composed	524:531	showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%)	509:779	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	5	20	theme	κ-carrageenan	1080:1092	arg1	film					1094:1097	κ-carrageenan film	1080:1097	κ-carrageenan film	1080:1097	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	2	21	theme	antioxidant	377:387	arg1	properties					407:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties	337:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films	337:465	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were compared for the first time.					
31739038	9	22	dep	suggested	1645:1653	arg1	used					1696:1699	used	1696:1699	be further used as active and intelligent packaging	1685:1735	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	5	23	theme	PPE	1158:1160	arg1	incorporation					1134:1146	the incorporation	1130:1146	the incorporation of PFE or PPE	1130:1160	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	3	24	theme	peonidin-3-O-	569:581	arg1	-glucoside					591:600	peonidin-3-O-(6-malon)-glucoside	569:600	peonidin-3-O-(6-malon)-glucoside (9.61%)	569:608	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	24	theme	peonidin-3-O-	569:581	arg1	%					607:607	9.61%	603:607	9.61%	603:607	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	4	25	theme	caffeic	944:950	arg1	%					962:962	4.37%	958:962	4.37%	958:962	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	4	25	theme	caffeic	944:950	arg1	acid					952:955	caffeic acid	944:955	caffeic acid (4.37%)	944:963	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	1	26	theme	intelligent	169:179	arg1	films					191:195	active and intelligent packaging films	158:195	active and intelligent packaging films	158:195	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	2	27	theme	films	461:465	arg1	properties					407:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties	337:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films	337:465	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were compared for the first time.					
31739038	4	28	theme	ellagic	991:997	arg1	acid					999:1002	ellagic acid	991:1002	ellagic acid (18.70%)	991:1011	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	4	28	theme	ellagic	991:997	arg1	%					1010:1010	18.70%	1005:1010	18.70%	1005:1010	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	6	29	theme	antimicrobial	1282:1294	arg1	properties					1296:1305	antimicrobial properties	1282:1305	antimicrobial properties	1282:1305	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	1	30	theme	packaging	181:189	arg1	films					191:195	active and intelligent packaging films	158:195	active and intelligent packaging films	158:195	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	0	31	theme	structural	18:27	arg1	properties					54:63	the structural, physical and functional properties	14:63	the structural, physical and functional properties of κ-carrageenan films	14:86	Comparison of the structural, physical and functional properties of κ-carrageenan films incorporated with pomegranate flesh and peel extracts.					
31739038	1	32	theme	pomegranate	279:289	arg1	PPE					305:307	PPE	305:307	PPE	305:307	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	1	32	theme	pomegranate	279:289	arg1	extract					296:302	pomegranate peel extract	279:302	pomegranate peel extract (PPE)	279:308	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	0	33	theme	peel	128:131	arg1	extracts					133:140	pomegranate flesh and peel extracts	106:140	extracts	133:140	Comparison of the structural, physical and functional properties of κ-carrageenan films incorporated with pomegranate flesh and peel extracts.					
31739038	5	34	theme	UV	1052:1053	arg1	light					1055:1059	UV light	1052:1059	UV light	1052:1059	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	2	35	theme	κ-carrageenan-PFE	421:437	arg1	films					461:465	κ-carrageenan-PFE and κ-carrageenan-PPE films	421:465	κ-carrageenan-PFE and κ-carrageenan-PPE films	421:465	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were compared for the first time.					
31739038	1	36	theme	peel	291:294	arg1	PPE					305:307	PPE	305:307	PPE	305:307	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	1	36	theme	peel	291:294	arg1	extract					296:302	pomegranate peel extract	279:302	pomegranate peel extract (PPE)	279:308	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	3	37	theme	6-acetyl	625:632	arg1	-glucoside					634:643	malvidin-3-O-(6-acetyl)-glucoside	611:643	malvidin-3-O-(6-acetyl)-glucoside (14.89%)	611:652	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	37	theme	6-acetyl	625:632	arg1	%					651:651	14.89%	646:651	14.89%	646:651	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	5	38	theme	barrier	1061:1067	arg1	ability					1069:1075	barrier ability	1061:1075	barrier ability	1061:1075	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	7	39	theme	abundant	1458:1465	arg1	anthocyanins					1467:1478	abundant anthocyanins	1458:1478	abundant anthocyanins in PFE	1458:1485	Nonetheless, κ-carrageenan-PFE films exhibited pH-sensitive property because of abundant anthocyanins in PFE.					
31739038	6	40	theme	UV	1248:1249	arg1	barrier					1257:1263	higher UV light barrier	1241:1263	higher UV light barrier	1241:1263	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	2	41	theme	first	489:493	arg1	time					495:498	the first time	485:498	the first time	485:498	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were compared for the first time.					
31739038	6	42	theme	κ-carrageenan-PFE	1312:1328	arg1	films					1330:1334	κ-carrageenan-PFE films	1312:1334	κ-carrageenan-PFE films	1312:1334	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	9	43	theme	intelligent	1715:1725	arg1	packaging					1727:1735	active and intelligent packaging	1704:1735	active and intelligent packaging	1704:1735	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	2	44	theme	pH-sensitive	363:374	arg1	properties					407:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties	337:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films	337:465	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were compared for the first time.					
31739038	9	45	theme	κ-carrageenan-PFE	1655:1671	arg1	films					1673:1677	κ-carrageenan-PFE films	1655:1677	κ-carrageenan-PFE films	1655:1677	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	3	46	theme	6-acetyl	728:735	arg1	-glucoside					737:746	pelargonidin-3-O-(6-acetyl)-glucoside	710:746	pelargonidin-3-O-(6-acetyl)-glucoside (9.51%)	710:754	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	46	theme	6-acetyl	728:735	arg1	%					753:753	9.51%	749:753	9.51%	749:753	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	47	theme	malvidin-3-O-	611:623	arg1	-glucoside					634:643	malvidin-3-O-(6-acetyl)-glucoside	611:643	malvidin-3-O-(6-acetyl)-glucoside (14.89%)	611:652	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	47	theme	malvidin-3-O-	611:623	arg1	%					651:651	14.89%	646:651	14.89%	646:651	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	2	48	theme	κ-carrageenan-PPE	443:459	arg1	films					461:465	κ-carrageenan-PFE and κ-carrageenan-PPE films	421:465	κ-carrageenan-PFE and κ-carrageenan-PPE films	421:465	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were compared for the first time.					
31739038	7	49	from	anthocyanins	1467:1478	arg1	PFE					1483:1485	PFE	1483:1485	PFE	1483:1485	Nonetheless, κ-carrageenan-PFE films exhibited pH-sensitive property because of abundant anthocyanins in PFE.					
31739038	2	50	theme	antimicrobial	393:405	arg1	properties					407:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties	337:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films	337:465	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were compared for the first time.					
31739038	0	51	theme	functional	43:52	arg1	properties					54:63	the structural, physical and functional properties	14:63	the structural, physical and functional properties of κ-carrageenan films	14:86	Comparison of the structural, physical and functional properties of κ-carrageenan films incorporated with pomegranate flesh and peel extracts.					
31739038	4	52	theme	gallic	809:814	arg1	acid					816:819	gallic acid	809:819	gallic acid (15.73%)	809:828	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	4	52	theme	gallic	809:814	arg1	%					827:827	15.73%	822:827	15.73%	822:827	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	3	53	theme	6-malon	583:589	arg1	-glucoside					591:600	peonidin-3-O-(6-malon)-glucoside	569:600	peonidin-3-O-(6-malon)-glucoside (9.61%)	569:608	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	53	theme	6-malon	583:589	arg1	%					607:607	9.61%	603:607	9.61%	603:607	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	6	54	theme	polyphenolic	1180:1191	arg1	compositions					1193:1204	different polyphenolic compositions	1170:1204	different polyphenolic compositions	1170:1204	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	4	55	theme	epicatechin	874:884	arg1	gallate					886:892	epicatechin gallate	874:892	epicatechin gallate (3.66%)	874:900	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	4	55	theme	epicatechin	874:884	arg1	%					899:899	3.66%	895:899	3.66%	895:899	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	3	56	theme	pelargonidin-3-O-	710:726	arg1	-glucoside					737:746	pelargonidin-3-O-(6-acetyl)-glucoside	710:746	pelargonidin-3-O-(6-acetyl)-glucoside (9.51%)	710:754	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	56	theme	pelargonidin-3-O-	710:726	arg1	%					753:753	9.51%	749:753	9.51%	749:753	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	57	theme	pelargonidin-3-O-	655:671	arg1	-glucoside					690:699	pelargonidin-3-O- (6″-ethylmalonyl)-glucoside	655:699	pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%)	655:707	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	57	theme	pelargonidin-3-O-	655:671	arg1	%					706:706	7.05%	702:706	7.05%	702:706	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	58	theme	14.89	646:650	arg1	-glucoside					634:643	malvidin-3-O-(6-acetyl)-glucoside	611:643	malvidin-3-O-(6-acetyl)-glucoside (14.89%)	611:652	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	3	58	theme	14.89	646:650	arg1	%					651:651	14.89%	646:651	14.89%	646:651	Results showed PFE was composed of cyanidin-3-O-glucoside (49.76%), peonidin-3-O-(6-malon)-glucoside (9.61%), malvidin-3-O-(6-acetyl)-glucoside (14.89%), pelargonidin-3-O- (6″-ethylmalonyl)-glucoside (7.05%), pelargonidin-3-O-(6-acetyl)-glucoside (9.51%) and isorhamnetin (9.18%).					
31739038	9	59	theme	κ-carrageenan-PPE	1746:1762	arg1	films					1764:1768	κ-carrageenan-PPE films	1746:1768	κ-carrageenan-PPE films	1746:1768	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	9	59	theme	κ-carrageenan-PPE	1746:1762	arg1	packaging					1798:1806	active packaging	1791:1806	active packaging in food industry	1791:1823	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	8	60	theme	functional	1514:1523	arg1	properties					1525:1534	the physical and functional properties	1497:1534	the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films	1497:1583	Notably, the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were closely related to the content of extract.					
31739038	8	60	theme	functional	1514:1523	arg1	related					1598:1604	related	1598:1604	related	1598:1604	Notably, the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were closely related to the content of extract.					
31739038	7	61	theme	κ-carrageenan-PFE	1391:1407	arg1	films					1409:1413	κ-carrageenan-PFE films	1391:1413	κ-carrageenan-PFE films	1391:1413	Nonetheless, κ-carrageenan-PFE films exhibited pH-sensitive property because of abundant anthocyanins in PFE.					
31739038	9	62	from	packaging	1798:1806	arg1	industry					1816:1823	food industry	1811:1823	food industry	1811:1823	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	5	63	theme	PFE	1151:1153	arg1	incorporation					1134:1146	the incorporation	1130:1146	the incorporation of PFE or PPE	1130:1160	The tensile strength, water vapor and UV light barrier ability of κ-carrageenan film were significantly promoted by the incorporation of PFE or PPE.					
31739038	6	64	theme	light	1251:1255	arg1	barrier					1257:1263	higher UV light barrier	1241:1263	higher UV light barrier	1241:1263	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	6	65	contain	possessed	1231:1239	arg1	films					1225:1229	κ-carrageenan-PPE films	1207:1229	κ-carrageenan-PPE films	1207:1229	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	6	65	contain	possessed	1231:1239	arg2	barrier					1257:1263	higher UV light barrier	1241:1263	higher UV light barrier	1241:1263	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	6	65	contain	possessed	1231:1239	arg2	properties					1296:1305	antimicrobial properties	1282:1305	antimicrobial properties	1282:1305	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	6	65	contain	possessed	1231:1239	arg2	antioxidant					1266:1276	antioxidant	1266:1276	antioxidant	1266:1276	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	9	66	theme	active	1791:1796	arg1	films					1764:1768	κ-carrageenan-PPE films	1746:1768	κ-carrageenan-PPE films	1746:1768	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	9	66	theme	active	1791:1796	arg1	packaging					1798:1806	active packaging	1791:1806	active packaging in food industry	1791:1823	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	6	67	theme	extract	1348:1354	arg1	levels					1370:1375	the same extract incorporation levels	1339:1375	the same extract incorporation levels	1339:1375	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	4	68	theme	vanillic	921:928	arg1	acid					930:933	vanillic acid	921:933	vanillic acid (7.82%)	921:941	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	4	68	theme	vanillic	921:928	arg1	%					940:940	7.82%	936:940	7.82%	936:940	PPE was mainly composed of gallic acid (15.73%), malvidin-3-O-(6-acetyl)-glucoside (4.07%), epicatechin gallate (3.66%), luteolin (5.69%), vanillic acid (7.82%), caffeic acid (4.37%), punicalagin (39.96%) and ellagic acid (18.70%).					
31739038	1	69	theme	polypheol-rich	229:242	arg1	PFE					271:273	PFE	271:273	PFE	271:273	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	1	69	theme	polypheol-rich	229:242	arg1	extract					262:268	polypheol-rich pomegranate flesh extract	229:268	polypheol-rich pomegranate flesh extract (PFE)	229:274	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	8	70	theme	physical	1501:1508	arg1	properties					1525:1534	the physical and functional properties	1497:1534	the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films	1497:1583	Notably, the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were closely related to the content of extract.					
31739038	8	70	theme	physical	1501:1508	arg1	related					1598:1604	related	1598:1604	related	1598:1604	Notably, the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were closely related to the content of extract.					
31739038	6	71	theme	different	1170:1178	arg1	compositions					1193:1204	different polyphenolic compositions	1170:1204	different polyphenolic compositions	1170:1204	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	6	72	theme	same	1343:1346	arg1	levels					1370:1375	the same extract incorporation levels	1339:1375	the same extract incorporation levels	1339:1375	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	6	73	theme	higher	1241:1246	arg1	barrier					1257:1263	higher UV light barrier	1241:1263	higher UV light barrier	1241:1263	Due to different polyphenolic compositions, κ-carrageenan-PPE films possessed higher UV light barrier, antioxidant and antimicrobial properties than κ-carrageenan-PFE films at the same extract incorporation levels.					
31739038	1	74	theme	pomegranate	244:254	arg1	PFE					271:273	PFE	271:273	PFE	271:273	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	1	74	theme	pomegranate	244:254	arg1	extract					262:268	polypheol-rich pomegranate flesh extract	229:268	polypheol-rich pomegranate flesh extract (PFE)	229:274	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	0	75	theme	properties	54:63	arg1	Comparison					0:9	Comparison	0:9	Comparison of the structural, physical and functional properties of κ-carrageenan films	0:86	Comparison of the structural, physical and functional properties of κ-carrageenan films incorporated with pomegranate flesh and peel extracts.					
31739038	1	76	theme	κ-carrageenan	315:327	arg1	matrix					329:334	κ-carrageenan matrix	315:334	κ-carrageenan matrix	315:334	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	8	77	theme	κ-carrageenan-PFE	1539:1555	arg1	films					1579:1583	κ-carrageenan-PFE and κ-carrageenan-PPE films	1539:1583	κ-carrageenan-PFE and κ-carrageenan-PPE films	1539:1583	Notably, the physical and functional properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were closely related to the content of extract.					
31739038	2	78	theme	physical	353:360	arg1	properties					407:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties	337:416	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films	337:465	The structural, physical, pH-sensitive, antioxidant and antimicrobial properties of κ-carrageenan-PFE and κ-carrageenan-PPE films were compared for the first time.					
31739038	9	79	theme	active	1704:1709	arg1	packaging					1727:1735	active and intelligent packaging	1704:1735	active and intelligent packaging	1704:1735	Our results suggested κ-carrageenan-PFE films could be further used as active and intelligent packaging, whereas κ-carrageenan-PPE films could be explored as active packaging in food industry.					
31739038	1	80	theme	flesh	256:260	arg1	PFE					271:273	PFE	271:273	PFE	271:273	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
31739038	1	80	theme	flesh	256:260	arg1	extract					262:268	polypheol-rich pomegranate flesh extract	229:268	polypheol-rich pomegranate flesh extract (PFE)	229:274	In this study, active and intelligent packaging films were developed by incorporating polypheol-rich pomegranate flesh extract (PFE) or pomegranate peel extract (PPE) into κ-carrageenan matrix.					
30830428	3	0	theme	potential	623:631	arg1	use					633:635	potential use	623:635	potential use of Dodamssal rice as an anti-obesity treatment	623:682	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	1	1	dep	Oryza	218:222	arg1	L.					231:232	Oryza sativa L.	218:232	Oryza sativa L.	218:232	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	0	2	theme	metabolic	168:176	arg1	profiling					178:186	metabolic profiling	168:186	metabolic profiling	168:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	4	3	theme	high-fat	769:776	arg1	diet					778:781	high-fat diet	769:781	high-fat diet (HFD)	769:787	METHODS Study canines were divided into three groups and fed either a regular diet, high-fat diet (HFD), or high-fat diet with Dodamssal rice (DoHFD).					
30830428	4	3	theme	high-fat	769:776	arg1	HFD					784:786	HFD	784:786	HFD	784:786	METHODS Study canines were divided into three groups and fed either a regular diet, high-fat diet (HFD), or high-fat diet with Dodamssal rice (DoHFD).					
30830428	3	4	theme	rice	650:653	arg1	effect					612:617	the effect	608:617	the effect	608:617	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	3	4	theme	rice	650:653	arg1	use					633:635	potential use	623:635	potential use of Dodamssal rice as an anti-obesity treatment	623:682	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	7	5	located	detected	1093:1100	arg2	RESULTS					1054:1060	RESULTS	1054:1060	RESULTS A total of 52 metabolites	1054:1086	RESULTS A total of 52 metabolites were detected in the canine feces.					
30830428	7	5	located	detected	1093:1100	arg1	feces					1116:1120	the canine feces	1105:1120	the canine feces	1105:1120	RESULTS A total of 52 metabolites were detected in the canine feces.					
30830428	3	6	theme	Targeted	494:501	arg1	profiles					513:520	Targeted metabolic profiles	494:520	OBJECTIVES Targeted metabolic profiles in canine feces	483:536	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	12	7	from	obesity	1729:1735	arg1	humans					1758:1763	humans	1758:1763	humans	1758:1763	Furthermore, Dodamssal rice may possibly be used not only for canines, but also to treat obesity in other animals and humans.					
30830428	12	7	from	obesity	1729:1735	arg1	animals					1746:1752	other animals	1740:1752	other animals	1740:1752	Furthermore, Dodamssal rice may possibly be used not only for canines, but also to treat obesity in other animals and humans.					
30830428	8	8	theme	C12:0	1191:1195	arg1	level					1182:1186	a significantly low level	1162:1186	a significantly low level of C12:0	1162:1195	In addition, HFD group feces contained a significantly low level of C12:0.					
30830428	8	9	theme	group	1140:1144	arg1	feces					1146:1150	HFD group feces	1136:1150	HFD group feces	1136:1150	In addition, HFD group feces contained a significantly low level of C12:0.					
30830428	9	10	theme	4-aminobutyric	1241:1254	arg1	acid					1256:1259	4-aminobutyric acid	1241:1259	4-aminobutyric acid	1241:1259	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	3	11	theme	canine	525:530	arg1	feces					532:536	canine feces	525:536	canine feces	525:536	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	4	12	theme	Dodamssal	812:820	arg1	DoHFD					828:832	DoHFD	828:832	DoHFD	828:832	METHODS Study canines were divided into three groups and fed either a regular diet, high-fat diet (HFD), or high-fat diet with Dodamssal rice (DoHFD).					
30830428	4	12	theme	Dodamssal	812:820	arg1	rice					822:825	Dodamssal rice	812:825	Dodamssal rice (DoHFD)	812:833	METHODS Study canines were divided into three groups and fed either a regular diet, high-fat diet (HFD), or high-fat diet with Dodamssal rice (DoHFD).					
30830428	0	13	theme	markers	147:153	arg1	rice					101:104	Dodamssal rice	91:104	Dodamssal rice	91:104	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	0	13	theme	markers	147:153	arg1	identification					114:127	the identification	110:127	the identification of discriminating markers in feces for metabolic profiling	110:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	3	14	theme	potential	565:573	arg1	biomarkers					575:584	potential biomarkers	565:584	potential biomarkers of RS	565:590	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	2	15	theme	diet	431:434	arg1	treatments					436:445	diet treatments	431:445	diet treatments	431:445	Recently, there has been an increase in the prevalence of obesity in canines; however, the information regarding diet treatments for such a condition is inadequate.					
30830428	3	16	from	profiles	513:520	arg1	feces					532:536	canine feces	525:536	canine feces	525:536	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	0	17	theme	high	46:49	arg1	starch					61:66	a high resistant starch	44:66	a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling	44:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	10	18	from	profiling	1388:1396	arg1	response					1421:1428	response	1421:1428	response to three diets	1421:1443	CONCLUSION For the first time, targeted metabolic profiling in the canine feces in response to three diets was performed.					
30830428	10	18	from	profiling	1388:1396	arg1	feces					1412:1416	the canine feces	1401:1416	the canine feces	1401:1416	CONCLUSION For the first time, targeted metabolic profiling in the canine feces in response to three diets was performed.					
30830428	2	19	from	prevalence	362:371	arg1	canines					387:393	canines	387:393	canines	387:393	Recently, there has been an increase in the prevalence of obesity in canines; however, the information regarding diet treatments for such a condition is inadequate.					
30830428	0	20	theme	starch	61:66	arg1	diet					68:71	a high resistant starch diet	44:71	a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling	44:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	9	21	theme	DoHFD	1202:1206	arg1	feces					1214:1218	The DoHFD group feces	1198:1218	The DoHFD group feces	1198:1218	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	1	22	theme	dietary	303:309	arg1	fiber					311:315	dietary fiber	303:315	dietary fiber	303:315	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	9	23	theme	higher	1224:1229	arg1	levels					1231:1236	higher levels	1224:1236	higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid	1224:1295	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	5	24	theme	chromatography	878:891	arg1	spectrometry					913:924	gas chromatography time-of-flight mass spectrometry	874:924	gas chromatography time-of-flight mass spectrometry	874:924	Fecal metabolites were analyzed using gas chromatography time-of-flight mass spectrometry and a gas chromatography-flame ionization detector.					
30830428	10	25	theme	first	1357:1361	arg1	time					1363:1366	the first time	1353:1366	the first time	1353:1366	CONCLUSION For the first time, targeted metabolic profiling in the canine feces in response to three diets was performed.					
30830428	0	26	theme	Trial	0:4	arg1	data					6:9	Trial data	0:9	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.	0:187	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	5	27	theme	Fecal	836:840	arg1	metabolites					842:852	Fecal metabolites	836:852	Fecal metabolites	836:852	Fecal metabolites were analyzed using gas chromatography time-of-flight mass spectrometry and a gas chromatography-flame ionization detector.					
30830428	4	28	theme	Study	693:697	arg1	canines					699:705	METHODS Study canines	685:705	METHODS Study canines	685:705	METHODS Study canines were divided into three groups and fed either a regular diet, high-fat diet (HFD), or high-fat diet with Dodamssal rice (DoHFD).					
30830428	12	29	theme	other	1740:1744	arg1	animals					1746:1752	other animals	1740:1752	other animals	1740:1752	Furthermore, Dodamssal rice may possibly be used not only for canines, but also to treat obesity in other animals and humans.					
30830428	1	30	theme	starch	269:274	arg1	levels					249:254	high levels	244:254	high levels of resistant starch (RS), which is a source of dietary fiber	244:315	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	6	31	theme	Multivariate	978:989	arg1	analyses					991:998	Multivariate analyses	978:998	Multivariate analyses	978:998	Multivariate analyses were used to analyze and visualize the obtained data.					
30830428	0	32	from	feces	158:162	arg1	rice					101:104	Dodamssal rice	91:104	Dodamssal rice	91:104	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	0	32	from	feces	158:162	arg1	identification					114:127	the identification	110:127	the identification of discriminating markers in feces for metabolic profiling	110:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	10	33	theme	metabolic	1378:1386	arg1	profiling					1388:1396	targeted metabolic profiling	1369:1396	targeted metabolic profiling in the canine feces in response to three diets	1369:1443	CONCLUSION For the first time, targeted metabolic profiling in the canine feces in response to three diets was performed.					
30830428	11	34	theme	diet	1583:1586	arg1	compositions					1588:1599	diet compositions	1583:1599	diet compositions	1583:1599	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	0	35	theme	anti-obesity	18:29	arg1	potential					31:39	the anti-obesity potential	14:39	the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling	14:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	9	36	theme	glucose	1262:1268	arg1	levels					1231:1236	higher levels	1224:1236	higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid	1224:1295	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	7	37	theme	metabolites	1076:1086	arg1	total					1064:1068	A total	1062:1068	RESULTS A total of 52 metabolites	1054:1086	RESULTS A total of 52 metabolites were detected in the canine feces.					
30830428	5	38	theme	gas	932:934	arg1	detector					968:975	a gas chromatography-flame ionization detector	930:975	a gas chromatography-flame ionization detector	930:975	Fecal metabolites were analyzed using gas chromatography time-of-flight mass spectrometry and a gas chromatography-flame ionization detector.					
30830428	10	39	dep	CONCLUSION	1338:1347	arg1	time					1363:1366	the first time	1353:1366	the first time	1353:1366	CONCLUSION For the first time, targeted metabolic profiling in the canine feces in response to three diets was performed.					
30830428	5	40	theme	ionization	957:966	arg1	detector					968:975	a gas chromatography-flame ionization detector	930:975	a gas chromatography-flame ionization detector	930:975	Fecal metabolites were analyzed using gas chromatography time-of-flight mass spectrometry and a gas chromatography-flame ionization detector.					
30830428	10	41	theme	canine	1405:1410	arg1	feces					1412:1416	the canine feces	1401:1416	the canine feces	1401:1416	CONCLUSION For the first time, targeted metabolic profiling in the canine feces in response to three diets was performed.					
30830428	11	42	theme	profiling	1475:1483	arg1	approach					1485:1492	This metabolic profiling approach	1460:1492	This metabolic profiling approach	1460:1492	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	11	42	theme	profiling	1475:1483	arg1	tool					1513:1516	a useful tool	1504:1516	a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines	1504:1637	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	9	43	theme	acid	1292:1295	arg1	levels					1231:1236	higher levels	1224:1236	higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid	1224:1295	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	5	44	theme	mass	908:911	arg1	spectrometry					913:924	gas chromatography time-of-flight mass spectrometry	874:924	gas chromatography time-of-flight mass spectrometry	874:924	Fecal metabolites were analyzed using gas chromatography time-of-flight mass spectrometry and a gas chromatography-flame ionization detector.					
30830428	2	45	from	increase	346:353	arg1	prevalence					362:371	the prevalence	358:371	the prevalence of obesity in canines	358:393	Recently, there has been an increase in the prevalence of obesity in canines; however, the information regarding diet treatments for such a condition is inadequate.					
30830428	0	46	from	rice	101:104	arg1	feces					158:162	feces	158:162	feces for metabolic profiling	158:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	3	47	theme	Dodamssal	640:648	arg1	rice					650:653	Dodamssal rice	640:653	Dodamssal rice	640:653	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	9	48	theme	other	1313:1317	arg1	groups					1319:1324	the other groups	1309:1324	the other groups (p < 0.05)	1309:1335	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	9	48	theme	other	1313:1317	arg1	p < 0.05					1327:1334	p < 0.05	1327:1334	p < 0.05	1327:1334	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	12	49	theme	Dodamssal	1653:1661	arg1	rice					1663:1666	Dodamssal rice	1653:1666	Dodamssal rice	1653:1666	Furthermore, Dodamssal rice may possibly be used not only for canines, but also to treat obesity in other animals and humans.					
30830428	0	50	from	markers	147:153	arg1	feces					158:162	feces	158:162	feces for metabolic profiling	158:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	1	51	theme	high	244:247	arg1	levels					249:254	high levels	244:254	high levels of resistant starch (RS), which is a source of dietary fiber	244:315	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	3	52	theme	anti-obesity	661:672	arg1	treatment					674:682	an anti-obesity treatment	658:682	an anti-obesity treatment	658:682	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	3	53	theme	metabolic	503:511	arg1	profiles					513:520	Targeted metabolic profiles	494:520	OBJECTIVES Targeted metabolic profiles in canine feces	483:536	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	8	54	contain	contained	1152:1160	arg2	feces					1146:1150	HFD group feces	1136:1150	HFD group feces	1136:1150	In addition, HFD group feces contained a significantly low level of C12:0.					
30830428	8	54	contain	contained	1152:1160	arg1	feces					1146:1150	HFD group feces	1136:1150	HFD group feces	1136:1150	In addition, HFD group feces contained a significantly low level of C12:0.					
30830428	8	54	contain	contained	1152:1160	arg2	level					1182:1186	a significantly low level	1162:1186	a significantly low level of C12:0	1162:1195	In addition, HFD group feces contained a significantly low level of C12:0.					
30830428	8	54	contain	contained	1152:1160	arg1	addition					1126:1133	addition	1126:1133	addition	1126:1133	In addition, HFD group feces contained a significantly low level of C12:0.					
30830428	7	55	dep	RESULTS	1054:1060	arg1	total					1064:1068	A total	1062:1068	RESULTS A total of 52 metabolites	1054:1086	RESULTS A total of 52 metabolites were detected in the canine feces.					
30830428	8	56	theme	low	1178:1180	arg1	level					1182:1186	a significantly low level	1162:1186	a significantly low level of C12:0	1162:1195	In addition, HFD group feces contained a significantly low level of C12:0.					
30830428	0	57	theme	discriminating	132:145	arg1	markers					147:153	discriminating markers	132:153	discriminating markers in feces for metabolic profiling	132:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	8	58	theme	HFD	1136:1138	arg1	feces					1146:1150	HFD group feces	1136:1150	HFD group feces	1136:1150	In addition, HFD group feces contained a significantly low level of C12:0.					
30830428	4	59	theme	high-fat	793:800	arg1	diet					802:805	high-fat diet	793:805	high-fat diet	793:805	METHODS Study canines were divided into three groups and fed either a regular diet, high-fat diet (HFD), or high-fat diet with Dodamssal rice (DoHFD).					
30830428	0	60	theme	resistant	51:59	arg1	starch					61:66	a high resistant starch	44:66	a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling	44:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	3	61	dep	OBJECTIVES	483:492	arg1	profiles					513:520	Targeted metabolic profiles	494:520	OBJECTIVES Targeted metabolic profiles in canine feces	483:536	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	11	62	theme	canines	1631:1637	arg1	treatment					1618:1626	anti-obesity treatment	1605:1626	anti-obesity treatment of canines	1605:1637	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	0	63	theme	diet	68:71	arg1	potential					31:39	the anti-obesity potential	14:39	the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling	14:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	9	64	theme	group	1208:1212	arg1	feces					1214:1218	The DoHFD group feces	1198:1218	The DoHFD group feces	1198:1218	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	0	65	theme	Dodamssal	91:99	arg1	rice					101:104	Dodamssal rice	91:104	Dodamssal rice	91:104	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	1	66	theme	fiber	311:315	arg1	source					293:298	a source	291:298	a source of dietary fiber	291:315	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	1	66	theme	fiber	311:315	arg1	starch					269:274	resistant starch	259:274	resistant starch (RS)	259:279	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	5	67	theme	gas	874:876	arg1	spectrometry					913:924	gas chromatography time-of-flight mass spectrometry	874:924	gas chromatography time-of-flight mass spectrometry	874:924	Fecal metabolites were analyzed using gas chromatography time-of-flight mass spectrometry and a gas chromatography-flame ionization detector.					
30830428	7	68	theme	canine	1109:1114	arg1	feces					1116:1120	the canine feces	1105:1120	the canine feces	1105:1120	RESULTS A total of 52 metabolites were detected in the canine feces.					
30830428	6	69	used	used	1005:1008	arg2	analyses					991:998	Multivariate analyses	978:998	Multivariate analyses	978:998	Multivariate analyses were used to analyze and visualize the obtained data.					
30830428	2	70	theme	obesity	376:382	arg1	prevalence					362:371	the prevalence	358:371	the prevalence of obesity in canines	358:393	Recently, there has been an increase in the prevalence of obesity in canines; however, the information regarding diet treatments for such a condition is inadequate.					
30830428	1	71	theme	resistant	259:267	arg1	starch					269:274	resistant starch	259:274	resistant starch (RS)	259:279	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	1	71	theme	resistant	259:267	arg1	source					293:298	a source	291:298	a source of dietary fiber	291:315	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	1	71	theme	resistant	259:267	arg1	RS					277:278	RS	277:278	RS	277:278	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	3	72	theme	RS	589:590	arg1	biomarkers					575:584	potential biomarkers	565:584	potential biomarkers of RS	565:590	OBJECTIVES Targeted metabolic profiles in canine feces were performed to identify potential biomarkers of RS and demonstrate the effect and potential use of Dodamssal rice as an anti-obesity treatment.					
30830428	4	73	theme	METHODS	685:691	arg1	canines					699:705	METHODS Study canines	685:705	METHODS Study canines	685:705	METHODS Study canines were divided into three groups and fed either a regular diet, high-fat diet (HFD), or high-fat diet with Dodamssal rice (DoHFD).					
30830428	9	74	contain	had	1220:1222	arg2	levels					1231:1236	higher levels	1224:1236	higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid	1224:1295	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	9	74	contain	had	1220:1222	arg1	feces					1214:1218	The DoHFD group feces	1198:1218	The DoHFD group feces	1198:1218	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	0	75	theme	potential	31:39	arg1	data					6:9	Trial data	0:9	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.	0:187	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	9	76	theme	acid	1256:1259	arg1	levels					1231:1236	higher levels	1224:1236	higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid	1224:1295	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	10	77	theme	targeted	1369:1376	arg1	profiling					1388:1396	targeted metabolic profiling	1369:1396	targeted metabolic profiling in the canine feces in response to three diets	1369:1443	CONCLUSION For the first time, targeted metabolic profiling in the canine feces in response to three diets was performed.					
30830428	5	78	theme	chromatography-flame	936:955	arg1	detector					968:975	a gas chromatography-flame ionization detector	930:975	a gas chromatography-flame ionization detector	930:975	Fecal metabolites were analyzed using gas chromatography time-of-flight mass spectrometry and a gas chromatography-flame ionization detector.					
30830428	11	79	theme	anti-obesity	1605:1616	arg1	treatment					1618:1626	anti-obesity treatment	1605:1626	anti-obesity treatment of canines	1605:1637	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	4	80	theme	regular	755:761	arg1	diet					763:766	a regular diet	753:766	a regular diet	753:766	METHODS Study canines were divided into three groups and fed either a regular diet, high-fat diet (HFD), or high-fat diet with Dodamssal rice (DoHFD).					
30830428	11	81	theme	metabolic	1465:1473	arg1	approach					1485:1492	This metabolic profiling approach	1460:1492	This metabolic profiling approach	1460:1492	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	11	81	theme	metabolic	1465:1473	arg1	tool					1513:1516	a useful tool	1504:1516	a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines	1504:1637	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	9	82	theme	3-hydroxybutyric	1275:1290	arg1	acid					1292:1295	3-hydroxybutyric acid	1275:1295	3-hydroxybutyric acid	1275:1295	The DoHFD group feces had higher levels of 4-aminobutyric acid, glucose, and 3-hydroxybutyric acid compared to the other groups (p < 0.05).					
30830428	0	83	from	identification	114:127	arg1	feces					158:162	feces	158:162	feces for metabolic profiling	158:186	Trial data of the anti-obesity potential of a high resistant starch diet for canines using Dodamssal rice and the identification of discriminating markers in feces for metabolic profiling.					
30830428	11	84	theme	compositions	1588:1599	arg1	effect					1573:1578	the effect	1569:1578	the effect of diet compositions for anti-obesity treatment of canines	1569:1637	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	11	85	theme	useful	1506:1511	arg1	approach					1485:1492	This metabolic profiling approach	1460:1492	This metabolic profiling approach	1460:1492	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	11	85	theme	useful	1506:1511	arg1	tool					1513:1516	a useful tool	1504:1516	a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines	1504:1637	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	6	86	theme	obtained	1039:1046	arg1	data					1048:1051	the obtained data	1035:1051	the obtained data	1035:1051	Multivariate analyses were used to analyze and visualize the obtained data.					
30830428	11	87	theme	discriminating	1528:1541	arg1	markers					1543:1549	discriminating markers	1528:1549	discriminating markers	1528:1549	This metabolic profiling approach should be a useful tool to detect discriminating markers as well as assess the effect of diet compositions for anti-obesity treatment of canines.					
30830428	1	88	dep	INTRODUCTION	189:200	arg1	Oryza					218:222	Oryza	218:222	Oryza	218:222	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	1	88	dep	INTRODUCTION	189:200	arg1	rice					212:215	rice	212:215	rice	212:215	INTRODUCTION Dodamssal rice (Oryza sativa L.) includes high levels of resistant starch (RS), which is a source of dietary fiber.					
30830428	12	89	used	used	1684:1687	arg2	rice					1663:1666	Dodamssal rice	1653:1666	Dodamssal rice	1653:1666	Furthermore, Dodamssal rice may possibly be used not only for canines, but also to treat obesity in other animals and humans.					
31078975	0	0	theme	Triclosan	71:79	arg1	removal					81:87	Triclosan removal	71:87	Triclosan removal	71:87	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	4	1	theme	composite	874:882	arg1	design					884:889	Central composite design	866:889	Central composite design (CCD) of RSM setup	866:908	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	4	1	theme	composite	874:882	arg1	CCD					892:894	CCD	892:894	CCD	892:894	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	3	2	theme	temperature	724:734	arg1	each					804:807	each	804:807	each	804:807	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	2	theme	temperature	724:734	arg1	factors					857:863	independent factors	845:863	independent factors	845:863	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	2	theme	temperature	724:734	arg1	effects					688:694	The effects	684:694	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min)	684:801	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	1	3	theme	neural	239:244	arg1	network					246:252	generalized regression neural network	216:252	generalized regression neural network (GRNN)	216:259	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	3	theme	neural	239:244	arg1	GRNN					255:258	GRNN	255:258	GRNN	255:258	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	6	4	theme	several	1174:1180	arg1	R2					1201:1202	R2	1201:1202	R2	1201:1202	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	4	theme	several	1174:1180	arg1	results					1165:1171	results	1165:1171	results	1165:1171	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	4	theme	several	1174:1180	arg1	statistics					1182:1191	several statistics	1174:1191	several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ)	1174:1514	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	1	5	theme	novel	388:392	arg1	complex					404:410	a novel inclusion complex	386:410	a novel inclusion complex (host-guest complex)	386:431	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	5	theme	novel	388:392	arg1	complex					424:430	host-guest complex	413:430	host-guest complex	413:430	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	6	6	theme	relative	1365:1372	arg1	error					1374:1378	average relative error	1357:1378	average relative error (ARE)	1357:1384	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	6	theme	relative	1365:1372	arg1	error					1309:1313	the absolute error	1296:1313	the absolute error (SAE)	1296:1319	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	4	7	theme	Central	866:872	arg1	design					884:889	Central composite design	866:889	Central composite design (CCD) of RSM setup	866:908	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	4	7	theme	Central	866:872	arg1	CCD					892:894	CCD	892:894	CCD	892:894	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	2	8	theme	poly	467:470	arg1	complex					506:512	poly(ethylene glycol) (PEG) host-guest complex	467:512	poly(ethylene glycol) (PEG) host-guest complex	467:512	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	6	9	theme	error	1405:1409	arg1	function					1411:1418	hybrid fractional error function	1387:1418	hybrid fractional error function	1387:1418	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	9	theme	error	1405:1409	arg1	error					1309:1313	the absolute error	1296:1313	the absolute error (SAE)	1296:1319	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	3	10	theme	independent	845:855	arg1	factors					857:863	independent factors	845:863	independent factors	845:863	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	10	theme	independent	845:855	arg1	effects					688:694	The effects	684:694	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min)	684:801	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	1	11	theme	inclusion	394:402	arg1	complex					404:410	a novel inclusion complex	386:410	a novel inclusion complex (host-guest complex)	386:431	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	11	theme	inclusion	394:402	arg1	complex					424:430	host-guest complex	413:430	host-guest complex	413:430	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	2	12	theme	technique	673:681	arg1	point					644:648	point	644:648	point	644:648	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	12	theme	technique	673:681	arg1	Raman					608:612	Raman	608:612	Raman	608:612	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	12	theme	technique	673:681	arg1	NMR					615:617	NMR	615:617	NMR	615:617	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	12	theme	technique	673:681	arg1	SEM					630:632	SEM	630:632	SEM	630:632	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	12	theme	technique	673:681	arg1	TEM					635:637	TEM	635:637	TEM	635:637	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	12	theme	technique	673:681	arg1	TGA					620:622	TGA	620:622	TGA	620:622	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	6	13	theme	fractional	1394:1403	arg1	function					1411:1418	hybrid fractional error function	1387:1418	hybrid fractional error function	1387:1418	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	13	theme	fractional	1394:1403	arg1	error					1309:1313	the absolute error	1296:1313	the absolute error (SAE)	1296:1319	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	14	theme	error	1309:1313	arg1	sum					1289:1291	sum	1289:1291	sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function	1289:1418	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	7	15	theme	ANFIS	1545:1549	arg1	approach					1551:1558	ANFIS approach	1545:1558	ANFIS approach	1545:1558	The results of ANFIS approach were found to be more trustworthy than GRNN model since better statistical analysis were attained.					
31078975	4	16	theme	setup	904:908	arg1	design					884:889	Central composite design	866:889	Central composite design (CCD) of RSM setup	866:908	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	4	16	theme	setup	904:908	arg1	CCD					892:894	CCD	892:894	CCD	892:894	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	0	17	theme	comprehensive	92:104	arg1	modeling					106:113	A comprehensive modeling and optimization-based adsorption study	90:153	modeling	106:113	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	6	18	theme	hybrid	1387:1392	arg1	function					1411:1418	hybrid fractional error function	1387:1418	hybrid fractional error function	1387:1418	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	18	theme	hybrid	1387:1392	arg1	error					1309:1313	the absolute error	1296:1313	the absolute error (SAE)	1296:1319	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	7	19	theme	approach	1551:1558	arg1	trustworthy					1582:1592	trustworthy	1582:1592	trustworthy	1582:1592	The results of ANFIS approach were found to be more trustworthy than GRNN model since better statistical analysis were attained.					
31078975	7	19	theme	approach	1551:1558	arg1	results					1534:1540	The results	1530:1540	The results of ANFIS approach	1530:1558	The results of ANFIS approach were found to be more trustworthy than GRNN model since better statistical analysis were attained.					
31078975	6	20	theme	squared	1241:1247	arg1	MSE					1256:1258	MSE	1256:1258	MSE	1256:1258	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	20	theme	squared	1241:1247	arg1	error					1249:1253	mean squared error	1236:1253	mean squared error (MSE)	1236:1259	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	1	21	theme	research	164:171	arg1	paper					173:177	this research paper	159:177	this research paper	159:177	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	22	theme	prediction	337:346	arg1	models					348:353	prediction models	337:353	prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex)	337:431	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	2	23	theme	carbon	541:546	arg1	MWCNT/PEG/β-CD					558:571	MWCNT/PEG/β-CD	558:571	MWCNT/PEG/β-CD	558:571	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	23	theme	carbon	541:546	arg1	nanotube					548:555	the multi walled carbon nanotube	524:555	the multi walled carbon nanotube (MWCNT/PEG/β-CD)	524:572	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	1	24	theme	host-guest	413:422	arg1	complex					404:410	a novel inclusion complex	386:410	a novel inclusion complex (host-guest complex)	386:431	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	24	theme	host-guest	413:422	arg1	complex					424:430	host-guest complex	413:430	host-guest complex	413:430	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	6	25	theme	root	1211:1214	arg1	error					1228:1232	root mean square error	1211:1232	root mean square error	1211:1232	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	25	theme	root	1211:1214	arg1	RMSE					1205:1208	RMSE	1205:1208	RMSE (root mean square error)	1205:1233	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	26	theme	mean	1267:1270	arg1	R2					1201:1202	R2	1201:1202	R2	1201:1202	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	26	theme	mean	1267:1270	arg1	error					1281:1285	mean absolute error	1267:1285	mean absolute error	1267:1285	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	27	theme	mean	1216:1219	arg1	error					1228:1232	root mean square error	1211:1232	root mean square error	1211:1232	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	27	theme	mean	1216:1219	arg1	RMSE					1205:1208	RMSE	1205:1208	RMSE (root mean square error)	1205:1233	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	28	theme	percentage	1441:1450	arg1	deviation					1461:1469	Marquart's percentage standard deviation	1430:1469	Marquart's percentage standard deviation	1430:1469	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	2	29	theme	walled	534:539	arg1	MWCNT/PEG/β-CD					558:571	MWCNT/PEG/β-CD	558:571	MWCNT/PEG/β-CD	558:571	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	29	theme	walled	534:539	arg1	nanotube					548:555	the multi walled carbon nanotube	524:555	the multi walled carbon nanotube (MWCNT/PEG/β-CD)	524:572	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	5	30	from	adsorption	1076:1085	arg1	nanocomposite					1111:1123	MWNT-PEG/β-CD nanocomposite	1097:1123	MWNT-PEG/β-CD nanocomposite	1097:1123	Moreover, the kinetic, isotherm equilibrium, and thermodynamic parameters of adsorption of TCS on MWNT-PEG/β-CD nanocomposite was examined.					
31078975	2	31	theme	ethylene	472:479	arg1	glycol					481:486	ethylene glycol	472:486	ethylene glycol	472:486	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	31	theme	ethylene	472:479	arg1	poly					467:470	poly	467:470	poly(ethylene glycol) (PEG) host-guest complex	467:512	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	32	theme	multi	528:532	arg1	MWCNT/PEG/β-CD					558:571	MWCNT/PEG/β-CD	558:571	MWCNT/PEG/β-CD	558:571	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	2	32	theme	multi	528:532	arg1	nanotube					548:555	the multi walled carbon nanotube	524:555	the multi walled carbon nanotube (MWCNT/PEG/β-CD)	524:572	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	7	33	theme	statistical	1623:1633	arg1	analysis					1635:1642	better statistical analysis	1616:1642	better statistical analysis	1616:1642	The results of ANFIS approach were found to be more trustworthy than GRNN model since better statistical analysis were attained.					
31078975	5	34	theme	TCS	1090:1092	arg1	adsorption					1076:1085	adsorption	1076:1085	adsorption of TCS on MWNT-PEG/β-CD nanocomposite	1076:1123	Moreover, the kinetic, isotherm equilibrium, and thermodynamic parameters of adsorption of TCS on MWNT-PEG/β-CD nanocomposite was examined.					
31078975	1	35	theme	response	180:187	arg1	methodology					197:207	response surface methodology	180:207	response surface methodology (RSM)	180:213	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	35	theme	response	180:187	arg1	RSM					210:212	RSM	210:212	RSM	210:212	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	4	36	with	combination	925:935	arg1	GRNN					952:955	GRNN	952:955	GRNN	952:955	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	4	36	with	combination	925:935	arg1	ANFIS					942:946	ANFIS	942:946	ANFIS	942:946	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	8	37	theme	little	1716:1721	arg1	time					1723:1726	a little time	1714:1726	a little time for modeling than the ANFIS approach	1714:1763	However, it was known that the GRNN is easier and take a little time for modeling than the ANFIS approach.					
31078975	0	38	dep	nanocomposite	53:65	arg1	modeling					106:113	A comprehensive modeling and optimization-based adsorption study	90:153	modeling	106:113	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	0	38	dep	nanocomposite	53:65	arg1	study					149:153	A comprehensive modeling and optimization-based adsorption study	90:153	study	149:153	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	6	39	theme	average	1337:1343	arg1	deviation					1345:1353	absolute average deviation	1328:1353	absolute average deviation	1328:1353	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	39	theme	average	1337:1343	arg1	AAD					1323:1325	%AAD	1322:1325	%AAD (absolute average deviation)	1322:1354	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	1	40	theme	surface	189:195	arg1	methodology					197:207	response surface methodology	180:207	response surface methodology (RSM)	180:213	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	40	theme	surface	189:195	arg1	RSM					210:212	RSM	210:212	RSM	210:212	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	0	41	theme	effective	9:17	arg1	nanocomposite					53:65	A highly effective, recyclable, and novel host-guest nanocomposite	0:65	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.	0:154	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	6	42	theme	standard	1452:1459	arg1	deviation					1461:1469	Marquart's percentage standard deviation	1430:1469	Marquart's percentage standard deviation	1430:1469	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	43	theme	absolute	1300:1307	arg1	error					1374:1378	average relative error	1357:1378	average relative error (ARE)	1357:1384	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	43	theme	absolute	1300:1307	arg1	SAE					1316:1318	SAE	1316:1318	SAE	1316:1318	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	43	theme	absolute	1300:1307	arg1	error					1309:1313	the absolute error	1296:1313	the absolute error (SAE)	1296:1319	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	43	theme	absolute	1300:1307	arg1	AAD					1323:1325	%AAD	1322:1325	%AAD (absolute average deviation)	1322:1354	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	43	theme	absolute	1300:1307	arg1	function					1411:1418	hybrid fractional error function	1387:1418	hybrid fractional error function	1387:1418	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	3	44	theme	concentration	753:765	arg1	each					804:807	each	804:807	each	804:807	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	44	theme	concentration	753:765	arg1	factors					857:863	independent factors	845:863	independent factors	845:863	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	44	theme	concentration	753:765	arg1	effects					688:694	The effects	684:694	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min)	684:801	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	45	theme	antibiotic	742:751	arg1	concentration					753:765	antibiotic concentration	742:765	antibiotic concentration (mg L-1)	742:774	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	45	theme	antibiotic	742:751	arg1	mg L-1					768:773	mg L-1	768:773	mg L-1	768:773	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	7	46	theme	GRNN	1599:1602	arg1	model					1604:1608	GRNN model	1599:1608	GRNN model	1599:1608	The results of ANFIS approach were found to be more trustworthy than GRNN model since better statistical analysis were attained.					
31078975	1	47	theme	Adaptive	266:273	arg1	ANFIS					305:309	ANFIS	305:309	ANFIS	305:309	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	47	theme	Adaptive	266:273	arg1	System					297:302	Adaptive Neuro-Fuzzy Inference System	266:302	Adaptive Neuro-Fuzzy Inference System (ANFIS)	266:310	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	0	48	theme	adsorption	138:147	arg1	study					149:153	A comprehensive modeling and optimization-based adsorption study	90:153	study	149:153	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	1	49	theme	Triclosan	359:367	arg1	removal					375:381	Triclosan (TCS) removal	359:381	Triclosan (TCS) removal by a novel inclusion complex (host-guest complex)	359:431	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	3	50	theme	MWCNT/PEG/β-CD	699:712	arg1	dose					714:717	MWCNT/PEG/β-CD dose	699:717	MWCNT/PEG/β-CD dose (g)	699:721	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	50	theme	MWCNT/PEG/β-CD	699:712	arg1	g					720:720	g	720:720	g	720:720	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	0	51	theme	recyclable	20:29	arg1	nanocomposite					53:65	A highly effective, recyclable, and novel host-guest nanocomposite	0:65	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.	0:154	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	1	52	theme	Neuro-Fuzzy	275:285	arg1	ANFIS					305:309	ANFIS	305:309	ANFIS	305:309	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	52	theme	Neuro-Fuzzy	275:285	arg1	System					297:302	Adaptive Neuro-Fuzzy Inference System	266:302	Adaptive Neuro-Fuzzy Inference System (ANFIS)	266:310	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	0	53	theme	optimization-based	119:136	arg1	study					149:153	A comprehensive modeling and optimization-based adsorption study	90:153	study	149:153	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	5	54	theme	MWNT-PEG/β-CD	1097:1109	arg1	nanocomposite					1111:1123	MWNT-PEG/β-CD nanocomposite	1097:1123	MWNT-PEG/β-CD nanocomposite	1097:1123	Moreover, the kinetic, isotherm equilibrium, and thermodynamic parameters of adsorption of TCS on MWNT-PEG/β-CD nanocomposite was examined.					
31078975	0	55	theme	novel	36:40	arg1	nanocomposite					53:65	A highly effective, recyclable, and novel host-guest nanocomposite	0:65	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.	0:154	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	1	56	theme	Inference	287:295	arg1	ANFIS					305:309	ANFIS	305:309	ANFIS	305:309	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	56	theme	Inference	287:295	arg1	System					297:302	Adaptive Neuro-Fuzzy Inference System	266:302	Adaptive Neuro-Fuzzy Inference System (ANFIS)	266:310	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	2	57	theme	host-guest	495:504	arg1	complex					506:512	poly(ethylene glycol) (PEG) host-guest complex	467:512	poly(ethylene glycol) (PEG) host-guest complex	467:512	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	5	58	dep	kinetic	1013:1019	arg1	the					1009:1011	the	1009:1011	the	1009:1011	Moreover, the kinetic, isotherm equilibrium, and thermodynamic parameters of adsorption of TCS on MWNT-PEG/β-CD nanocomposite was examined.					
31078975	8	59	theme	ANFIS	1750:1754	arg1	approach					1756:1763	the ANFIS approach	1746:1763	the ANFIS approach	1746:1763	However, it was known that the GRNN is easier and take a little time for modeling than the ANFIS approach.					
31078975	3	60	theme	sonication	781:790	arg1	min					798:800	min	798:800	min	798:800	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	60	theme	sonication	781:790	arg1	time					792:795	sonication time	781:795	sonication time (min)	781:801	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	6	61	theme	average	1357:1363	arg1	error					1374:1378	average relative error	1357:1378	average relative error (ARE)	1357:1384	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	61	theme	average	1357:1363	arg1	error					1309:1313	the absolute error	1296:1313	the absolute error (SAE)	1296:1319	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	62	theme	mean	1236:1239	arg1	MSE					1256:1258	MSE	1256:1258	MSE	1256:1258	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	62	theme	mean	1236:1239	arg1	error					1249:1253	mean squared error	1236:1253	mean squared error (MSE)	1236:1259	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	4	63	theme	evaluation	978:987	arg1	purposes					989:996	evaluation purposes	978:996	evaluation purposes	978:996	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	6	64	theme	square	1221:1226	arg1	error					1228:1232	root mean square error	1211:1232	root mean square error	1211:1232	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	64	theme	square	1221:1226	arg1	RMSE					1205:1208	RMSE	1205:1208	RMSE (root mean square error)	1205:1233	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	3	65	theme	dose	714:717	arg1	each					804:807	each	804:807	each	804:807	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	65	theme	dose	714:717	arg1	factors					857:863	independent factors	845:863	independent factors	845:863	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	65	theme	dose	714:717	arg1	effects					688:694	The effects	684:694	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min)	684:801	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	6	66	dep	error	1374:1378	arg1	ARE					1381:1383	ARE	1381:1383	ARE	1381:1383	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	67	theme	%	1322:1322	arg1	deviation					1345:1353	absolute average deviation	1328:1353	absolute average deviation	1328:1353	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	67	theme	%	1322:1322	arg1	error					1309:1313	the absolute error	1296:1313	the absolute error (SAE)	1296:1319	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	67	theme	%	1322:1322	arg1	AAD					1323:1325	%AAD	1322:1325	%AAD (absolute average deviation)	1322:1354	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	7	68	theme	better	1616:1621	arg1	analysis					1635:1642	better statistical analysis	1616:1642	better statistical analysis	1616:1642	The results of ANFIS approach were found to be more trustworthy than GRNN model since better statistical analysis were attained.					
31078975	6	69	theme	absolute	1328:1335	arg1	deviation					1345:1353	absolute average deviation	1328:1353	absolute average deviation	1328:1353	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	69	theme	absolute	1328:1335	arg1	AAD					1323:1325	%AAD	1322:1325	%AAD (absolute average deviation)	1322:1354	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	2	70	theme	pHpzc	666:670	arg1	technique					673:681	zero charge (pHpzc) technique	653:681	zero charge (pHpzc) technique	653:681	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	5	71	theme	adsorption	1076:1085	arg1	kinetic					1013:1019	kinetic	1013:1019	kinetic	1013:1019	Moreover, the kinetic, isotherm equilibrium, and thermodynamic parameters of adsorption of TCS on MWNT-PEG/β-CD nanocomposite was examined.					
31078975	6	72	theme	Chi-square	1493:1502	arg1	measure					1504:1510	Pearson's Chi-square measure	1483:1510	Pearson's Chi-square measure	1483:1510	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	4	73	theme	RSM	900:902	arg1	setup					904:908	RSM setup	900:908	RSM setup	900:908	Central composite design (CCD) of RSM setup was applied in combination with ANFIS and GRNN training dataset for evaluation purposes.					
31078975	5	74	theme	thermodynamic	1048:1060	arg1	parameters					1062:1071	thermodynamic parameters	1048:1071	thermodynamic parameters	1048:1071	Moreover, the kinetic, isotherm equilibrium, and thermodynamic parameters of adsorption of TCS on MWNT-PEG/β-CD nanocomposite was examined.					
31078975	2	75	theme	charge	658:663	arg1	technique					673:681	zero charge (pHpzc) technique	653:681	zero charge (pHpzc) technique	653:681	Hence, β-cyclodextrin (β-CD) and poly(ethylene glycol) (PEG) host-guest complex loaded on the multi walled carbon nanotube (MWCNT/PEG/β-CD) was prepared and characterized by Raman, NMR, TGA, XRD, SEM, TEM, and point of zero charge (pHpzc) technique.					
31078975	1	76	theme	generalized	216:226	arg1	network					246:252	generalized regression neural network	216:252	generalized regression neural network (GRNN)	216:259	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	76	theme	generalized	216:226	arg1	GRNN					255:258	GRNN	255:258	GRNN	255:258	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	0	77	theme	host-guest	42:51	arg1	nanocomposite					53:65	A highly effective, recyclable, and novel host-guest nanocomposite	0:65	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.	0:154	A highly effective, recyclable, and novel host-guest nanocomposite for Triclosan removal: A comprehensive modeling and optimization-based adsorption study.					
31078975	5	78	theme	isotherm	1022:1029	arg1	equilibrium					1031:1041	isotherm equilibrium	1022:1041	isotherm equilibrium	1022:1041	Moreover, the kinetic, isotherm equilibrium, and thermodynamic parameters of adsorption of TCS on MWNT-PEG/β-CD nanocomposite was examined.					
31078975	3	79	theme	time	792:795	arg1	each					804:807	each	804:807	each	804:807	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	79	theme	time	792:795	arg1	factors					857:863	independent factors	845:863	independent factors	845:863	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	3	79	theme	time	792:795	arg1	effects					688:694	The effects	684:694	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min)	684:801	The effects of MWCNT/PEG/β-CD dose (g), temperature (°C), antibiotic concentration (mg L-1), and sonication time (min), each at five levels were investigated as independent factors.					
31078975	6	80	theme	absolute	1272:1279	arg1	R2					1201:1202	R2	1201:1202	R2	1201:1202	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	6	80	theme	absolute	1272:1279	arg1	error					1281:1285	mean absolute error	1267:1285	mean absolute error	1267:1285	To assess the accuracy of results, several statistics such as R2, RMSE (root mean square error), mean squared error (MSE), MAE (mean absolute error), sum of the absolute error (SAE), %AAD (absolute average deviation), average relative error (ARE), hybrid fractional error function (HYBRID), Marquart's percentage standard deviation (MPSD), and Pearson's Chi-square measure (χ) were checked.					
31078975	1	81	theme	regression	228:237	arg1	network					246:252	generalized regression neural network	216:252	generalized regression neural network (GRNN)	216:259	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
31078975	1	81	theme	regression	228:237	arg1	GRNN					255:258	GRNN	255:258	GRNN	255:258	In this research paper, response surface methodology (RSM), generalized regression neural network (GRNN), and Adaptive Neuro-Fuzzy Inference System (ANFIS) were employed to develop prediction models for Triclosan (TCS) removal by a novel inclusion complex (host-guest complex).					
29522550	6	0	theme	site-	918:922	arg1	manner					941:946	a site- and age-specific manner	916:946	a site- and age-specific manner	916:946	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	1	theme	study	830:834	arg1	aim					818:820	the aim	814:820	the aim of this study	814:834	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	2	theme	knee	899:902	arg1	meniscus					904:911	the equine knee meniscus	888:911	the equine knee meniscus in a site- and age-specific manner	888:946	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	5	3	theme	meniscus	648:655	arg1	degeneration					657:668	meniscus degeneration	648:668	meniscus degeneration	648:668	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	10	4	theme	significant	1417:1427	arg1	differences					1429:1439	neither site nor age related significant differences	1388:1439	neither site nor age related significant differences	1388:1439	Stiffness and energy loss showed neither site nor age related significant differences.					
29522550	6	5	from	composition	859:869	arg1	manner					941:946	a site- and age-specific manner	916:946	a site- and age-specific manner	916:946	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	6	theme	potential	976:984	arg1	properties					1014:1023	potential site-specific biomechanical properties	976:1023	potential site-specific biomechanical properties	976:1023	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	11	7	theme	continued	1552:1560	arg1	research					1562:1569	continued research	1552:1569	continued research in this field	1552:1583	The macroscopic and histologic similarities between equine and human menisci described in this study, support continued research in this field.					
29522550	6	8	from	structure	875:883	arg1	manner					941:946	a site- and age-specific manner	916:946	a site- and age-specific manner	916:946	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	5	9	theme	suitable	726:733	arg1	model					756:760	suitable translational animal model	726:760	suitable translational animal model for (human) meniscus tissue engineering	726:800	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	4	10	theme	equine	438:443	arg1	topography					454:463	equine meniscus topography	438:463	equine meniscus topography	438:463	However, the influence of equine meniscus topography and composition on its biomechanical properties is yet unknown.					
29522550	5	11	theme	animal	749:754	arg1	model					756:760	suitable translational animal model	726:760	suitable translational animal model for (human) meniscus tissue engineering	726:800	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	9	12	from	SH	1292:1293	arg1	SH					1342:1343	SH	1342:1343	SH with age	1342:1352	The SH was found to be subjected to both age and site-specific changes, with an overall higher SH of the tibial meniscus surface and increase in SH with age.					
29522550	4	13	theme	topography	454:463	arg1	influence					425:433	the influence	421:433	the influence of equine meniscus topography and composition on its biomechanical properties	421:511	However, the influence of equine meniscus topography and composition on its biomechanical properties is yet unknown.					
29522550	4	13	theme	topography	454:463	arg1	unknown					520:526	unknown	520:526	unknown	520:526	However, the influence of equine meniscus topography and composition on its biomechanical properties is yet unknown.					
29522550	4	14	from	influence	425:433	arg1	properties					502:511	its biomechanical properties	484:511	its biomechanical properties	484:511	However, the influence of equine meniscus topography and composition on its biomechanical properties is yet unknown.					
29522550	2	15	theme	cranial	217:223	arg1	horn					225:228	the cranial horn	213:228	the cranial horn of the equine medial meniscus	213:258	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	7	16	theme	meniscus	1030:1037	arg1	architecture					1039:1050	The meniscus architecture	1026:1050	The meniscus architecture	1026:1050	The meniscus architecture was investigated histologically.					
29522550	1	17	theme	joint	128:132	arg1	injuries					99:106	the most common injuries	83:106	the most common injuries of the femorotibial joint in both human and equine patients	83:166	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
29522550	6	18	theme	age-specific	928:939	arg1	manner					941:946	a site- and age-specific manner	916:946	a site- and age-specific manner	916:946	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	2	19	theme	meniscus	251:258	arg1	horn					225:228	the cranial horn	213:228	the cranial horn of the equine medial meniscus	213:258	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	5	20	theme	meniscus	774:781	arg1	engineering					790:800	(human) meniscus tissue engineering	766:800	(human) meniscus tissue engineering	766:800	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	2	21	theme	equine	237:242	arg1	meniscus					251:258	the equine medial meniscus	233:258	the equine medial meniscus	233:258	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	5	22	theme	better	531:536	arg1	understanding					538:550	A better understanding	529:550	A better understanding of equine meniscus composition and biomechanics	529:598	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	11	23	from	research	1562:1569	arg1	field					1579:1583	this field	1574:1583	this field	1574:1583	The macroscopic and histologic similarities between equine and human menisci described in this study, support continued research in this field.					
29522550	3	24	dep	site-	385:389	arg1	specific					402:409	specific	402:409	specific	402:409	Biomechanical properties of human menisci are site- and depth- specific.					
29522550	9	25	theme	meniscus	1309:1316	arg1	surface					1318:1324	the tibial meniscus surface	1298:1324	the tibial meniscus surface	1298:1324	The SH was found to be subjected to both age and site-specific changes, with an overall higher SH of the tibial meniscus surface and increase in SH with age.					
29522550	8	26	theme	Biomechanical	1085:1097	arg1	testing					1099:1105	Biomechanical testing	1085:1105	Biomechanical testing	1085:1105	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	5	27	theme	veterinary	623:632	arg1	therapies					634:642	not only veterinary therapies	614:642	not only veterinary therapies for meniscus degeneration or injuries	614:680	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	6	28	theme	biomechanical	1000:1012	arg1	properties					1014:1023	potential site-specific biomechanical properties	976:1023	potential site-specific biomechanical properties	976:1023	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	3	29	theme	Biomechanical	339:351	arg1	properties					353:362	Biomechanical properties	339:362	Biomechanical properties of human menisci	339:379	Biomechanical properties of human menisci are site- and depth- specific.					
29522550	4	30	theme	composition	469:479	arg1	influence					425:433	the influence	421:433	the influence of equine meniscus topography and composition on its biomechanical properties	421:511	However, the influence of equine meniscus topography and composition on its biomechanical properties is yet unknown.					
29522550	4	30	theme	composition	469:479	arg1	unknown					520:526	unknown	520:526	unknown	520:526	However, the influence of equine meniscus topography and composition on its biomechanical properties is yet unknown.					
29522550	11	31	theme	macroscopic	1446:1456	arg1	similarities					1473:1484	The macroscopic and histologic similarities	1442:1484	The macroscopic and histologic similarities	1442:1484	The macroscopic and histologic similarities between equine and human menisci described in this study, support continued research in this field.					
29522550	5	32	theme	human	767:771	arg1	engineering					790:800	(human) meniscus tissue engineering	766:800	(human) meniscus tissue engineering	766:800	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	6	33	with	composition	859:869	arg1	properties					1014:1023	potential site-specific biomechanical properties	976:1023	potential site-specific biomechanical properties	976:1023	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	0	34	theme	Structure-Function	0:17	arg1	relationships					19:31	Structure-Function relationships	0:31	Structure-Function relationships of equine menisci	0:49	Structure-Function relationships of equine menisci.					
29522550	6	35	with	structure	875:883	arg1	properties					1014:1023	potential site-specific biomechanical properties	976:1023	potential site-specific biomechanical properties	976:1023	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	3	36	theme	menisci	373:379	arg1	properties					353:362	Biomechanical properties	339:362	Biomechanical properties of human menisci	339:379	Biomechanical properties of human menisci are site- and depth- specific.					
29522550	8	37	theme	shore	1134:1138	arg1	SH					1150:1151	SH	1150:1151	SH	1150:1151	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	8	37	theme	shore	1134:1138	arg1	hardness					1140:1147	the shore hardness	1130:1147	the shore hardness (SH)	1130:1152	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	5	38	theme	meniscus	562:569	arg1	composition					571:581	equine meniscus composition	555:581	equine meniscus composition	555:581	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	11	39	theme	histologic	1462:1471	arg1	similarities					1473:1484	The macroscopic and histologic similarities	1442:1484	The macroscopic and histologic similarities	1442:1484	The macroscopic and histologic similarities between equine and human menisci described in this study, support continued research in this field.					
29522550	10	40	dep	differences	1429:1439	arg1	neither					1388:1394	neither	1388:1394	neither	1388:1394	Stiffness and energy loss showed neither site nor age related significant differences.					
29522550	10	40	dep	differences	1429:1439	arg1	related					1409:1415	related	1409:1415	related	1409:1415	Stiffness and energy loss showed neither site nor age related significant differences.					
29522550	0	41	theme	menisci	43:49	arg1	relationships					19:31	Structure-Function relationships	0:31	Structure-Function relationships of equine menisci	0:49	Structure-Function relationships of equine menisci.					
29522550	6	42	from	manner	941:946	arg1	structure					875:883	structure	875:883	structure	875:883	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	42	from	manner	941:946	arg1	relationship					958:969	their relationship	952:969	their relationship with potential site-specific biomechanical properties	952:1023	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	42	from	manner	941:946	arg1	composition					859:869	composition	859:869	composition	859:869	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	2	43	theme	medial	326:331	arg1	horn					333:336	the human posterior medial horn	306:336	the human posterior medial horn	306:336	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	8	44	theme	stiffness	1155:1163	arg1	evaluation					1116:1125	evaluation	1116:1125	evaluation of the shore hardness (SH), stiffness and energy loss of the menisci	1116:1194	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	2	45	theme	ensuing	193:199	arg1	injuries					201:208	ensuing injuries	193:208	ensuing injuries	193:208	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	1	46	from	injuries	99:106	arg1	patients					159:166	both human and equine patients	137:166	both human and equine patients	137:166	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
29522550	8	47	theme	energy	1169:1174	arg1	loss					1176:1179	energy loss	1169:1179	energy loss	1169:1179	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	10	48	dep	neither	1388:1394	arg1	site					1396:1399	site	1396:1399	site	1396:1399	Stiffness and energy loss showed neither site nor age related significant differences.					
29522550	8	49	theme	menisci	1188:1194	arg1	SH					1150:1151	SH	1150:1151	SH	1150:1151	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	8	49	theme	menisci	1188:1194	arg1	stiffness					1155:1163	stiffness	1155:1163	stiffness	1155:1163	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	8	49	theme	menisci	1188:1194	arg1	hardness					1140:1147	the shore hardness	1130:1147	the shore hardness (SH)	1130:1152	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	8	49	theme	menisci	1188:1194	arg1	loss					1176:1179	energy loss	1169:1179	energy loss	1169:1179	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	6	50	theme	meniscus	904:911	arg1	structure					875:883	structure	875:883	structure	875:883	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	50	theme	meniscus	904:911	arg1	relationship					958:969	their relationship	952:969	their relationship with potential site-specific biomechanical properties	952:1023	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	50	theme	meniscus	904:911	arg1	composition					859:869	composition	859:869	composition	859:869	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	51	with	relationship	958:969	arg1	properties					1014:1023	potential site-specific biomechanical properties	976:1023	potential site-specific biomechanical properties	976:1023	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	52	from	meniscus	904:911	arg1	manner					941:946	a site- and age-specific manner	916:946	a site- and age-specific manner	916:946	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	6	53	theme	equine	892:897	arg1	meniscus					904:911	the equine knee meniscus	888:911	the equine knee meniscus in a site- and age-specific manner	888:946	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	2	54	theme	human	310:314	arg1	horn					333:336	the human posterior medial horn	306:336	the human posterior medial horn	306:336	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	9	55	from	increase	1330:1337	arg1	SH					1342:1343	SH	1342:1343	SH with age	1342:1352	The SH was found to be subjected to both age and site-specific changes, with an overall higher SH of the tibial meniscus surface and increase in SH with age.					
29522550	6	56	theme	site-specific	986:998	arg1	properties					1014:1023	potential site-specific biomechanical properties	976:1023	potential site-specific biomechanical properties	976:1023	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	9	57	with	SH	1342:1343	arg1	age					1350:1352	age	1350:1352	age	1350:1352	The SH was found to be subjected to both age and site-specific changes, with an overall higher SH of the tibial meniscus surface and increase in SH with age.					
29522550	4	58	theme	meniscus	445:452	arg1	topography					454:463	equine meniscus topography	438:463	equine meniscus topography	438:463	However, the influence of equine meniscus topography and composition on its biomechanical properties is yet unknown.					
29522550	5	59	theme	translational	735:747	arg1	model					756:760	suitable translational animal model	726:760	suitable translational animal model for (human) meniscus tissue engineering	726:800	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	9	60	theme	site-specific	1246:1258	arg1	changes					1260:1266	site-specific changes	1246:1266	site-specific changes	1246:1266	The SH was found to be subjected to both age and site-specific changes, with an overall higher SH of the tibial meniscus surface and increase in SH with age.					
29522550	2	61	theme	horn	225:228	arg1	forces					182:187	Pathological forces	169:187	Pathological forces	169:187	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	2	61	theme	horn	225:228	arg1	injuries					201:208	ensuing injuries	193:208	ensuing injuries	193:208	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	5	62	theme	equine	555:560	arg1	composition					571:581	equine meniscus composition	555:581	equine meniscus composition	555:581	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	1	63	theme	femorotibial	115:126	arg1	joint					128:132	the femorotibial joint	111:132	the femorotibial joint in both human and equine patients	111:166	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
29522550	5	64	theme	composition	571:581	arg1	understanding					538:550	A better understanding	529:550	A better understanding of equine meniscus composition and biomechanics	529:598	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	9	65	theme	higher	1285:1290	arg1	SH					1292:1293	an overall higher SH	1274:1293	an overall higher SH of the tibial meniscus surface	1274:1324	The SH was found to be subjected to both age and site-specific changes, with an overall higher SH of the tibial meniscus surface and increase in SH with age.					
29522550	2	66	theme	medial	244:249	arg1	meniscus					251:258	the equine medial meniscus	233:258	the equine medial meniscus	233:258	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	5	67	theme	tissue	783:788	arg1	engineering					790:800	(human) meniscus tissue engineering	766:800	(human) meniscus tissue engineering	766:800	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	11	68	dep	equine	1494:1499	arg1	menisci					1511:1517	menisci	1511:1517	menisci	1511:1517	The macroscopic and histologic similarities between equine and human menisci described in this study, support continued research in this field.					
29522550	9	69	theme	tibial	1302:1307	arg1	surface					1318:1324	the tibial meniscus surface	1298:1324	the tibial meniscus surface	1298:1324	The SH was found to be subjected to both age and site-specific changes, with an overall higher SH of the tibial meniscus surface and increase in SH with age.					
29522550	1	70	from	patients	159:166	arg1	injuries					99:106	the most common injuries	83:106	the most common injuries of the femorotibial joint in both human and equine patients	83:166	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
29522550	3	71	theme	human	367:371	arg1	menisci					373:379	human menisci	367:379	human menisci	367:379	Biomechanical properties of human menisci are site- and depth- specific.					
29522550	1	72	dep	both	137:140	arg1	human					142:146	human	142:146	human	142:146	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
29522550	9	73	theme	surface	1318:1324	arg1	SH					1292:1293	an overall higher SH	1274:1293	an overall higher SH of the tibial meniscus surface	1274:1324	The SH was found to be subjected to both age and site-specific changes, with an overall higher SH of the tibial meniscus surface and increase in SH with age.					
29522550	9	73	theme	surface	1318:1324	arg1	increase					1330:1337	increase	1330:1337	increase in SH with age	1330:1352	The SH was found to be subjected to both age and site-specific changes, with an overall higher SH of the tibial meniscus surface and increase in SH with age.					
29522550	4	74	theme	biomechanical	488:500	arg1	properties					502:511	its biomechanical properties	484:511	its biomechanical properties	484:511	However, the influence of equine meniscus topography and composition on its biomechanical properties is yet unknown.					
29522550	5	75	theme	biomechanics	587:598	arg1	understanding					538:550	A better understanding	529:550	A better understanding of equine meniscus composition and biomechanics	529:598	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	1	76	theme	common	92:97	arg1	injuries					99:106	the most common injuries	83:106	the most common injuries of the femorotibial joint in both human and equine patients	83:166	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
29522550	1	77	dep	patients	159:166	arg1	both					137:140	both	137:140	both	137:140	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
29522550	1	77	dep	patients	159:166	arg1	equine					152:157	equine	152:157	equine	152:157	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
29522550	0	78	theme	equine	36:41	arg1	menisci					43:49	equine menisci	36:49	equine menisci	36:49	Structure-Function relationships of equine menisci.					
29522550	8	79	theme	hardness	1140:1147	arg1	evaluation					1116:1125	evaluation	1116:1125	evaluation of the shore hardness (SH), stiffness and energy loss of the menisci	1116:1194	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	1	80	from	joint	128:132	arg1	patients					159:166	both human and equine patients	137:166	both human and equine patients	137:166	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
29522550	2	81	located	observed	294:301	arg1	horn					333:336	the human posterior medial horn	306:336	the human posterior medial horn	306:336	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	2	81	located	observed	294:301	arg2	those					288:292	those	288:292	those	288:292	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	6	82	from	relationship	958:969	arg1	manner					941:946	a site- and age-specific manner	916:946	a site- and age-specific manner	916:946	Therefore, the aim of this study was to investigate the composition and structure of the equine knee meniscus in a site- and age-specific manner and their relationship with potential site-specific biomechanical properties.					
29522550	2	83	theme	Pathological	169:180	arg1	forces					182:187	Pathological forces	169:187	Pathological forces	169:187	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	5	84	dep	therapies	634:642	arg1	only					618:621	only	618:621	only	618:621	A better understanding of equine meniscus composition and biomechanics could advance not only veterinary therapies for meniscus degeneration or injuries, but also further substantiate the horse as suitable translational animal model for (human) meniscus tissue engineering.					
29522550	10	85	theme	energy	1369:1374	arg1	loss					1376:1379	energy loss	1369:1379	energy loss	1369:1379	Stiffness and energy loss showed neither site nor age related significant differences.					
29522550	2	86	theme	posterior	316:324	arg1	horn					333:336	the human posterior medial horn	306:336	the human posterior medial horn	306:336	Pathological forces and ensuing injuries of the cranial horn of the equine medial meniscus are considered analogous to those observed in the human posterior medial horn.					
29522550	8	87	theme	loss	1176:1179	arg1	evaluation					1116:1125	evaluation	1116:1125	evaluation of the shore hardness (SH), stiffness and energy loss of the menisci	1116:1194	Biomechanical testing included evaluation of the shore hardness (SH), stiffness and energy loss of the menisci.					
29522550	1	88	theme	Meniscal	52:59	arg1	pathologies					61:71	Meniscal pathologies	52:71	Meniscal pathologies	52:71	Meniscal pathologies are among the most common injuries of the femorotibial joint in both human and equine patients.					
31808500	0	0	theme	alginate	86:93	arg1	hydrogel					95:102	injectable alginate hydrogel	75:102	injectable alginate hydrogel	75:102	Sustained release of bioactive IGF-1 from a silk fibroin microsphere-based injectable alginate hydrogel for the treatment of myocardial infarction.					
31808500	7	1	theme	peripheral	1545:1554	arg1	region					1556:1561	the peripheral region	1541:1561	the peripheral region of a MI rat model	1541:1579	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	3	2	theme	different	711:719	arg1	concentrations					721:734	different concentrations	711:734	different concentrations (1.0-2.5%)	711:745	First, IGF-1 was physically adsorbed onto SF microspheres prepared by the coaxial needle system, and these IGF-1-containing microspheres were subsequently encapsulated into sodium alginate solutions at different concentrations (1.0-2.5%).					
31808500	3	2	theme	different	711:719	arg1	%					744:744	1.0-2.5%	737:744	1.0-2.5%	737:744	First, IGF-1 was physically adsorbed onto SF microspheres prepared by the coaxial needle system, and these IGF-1-containing microspheres were subsequently encapsulated into sodium alginate solutions at different concentrations (1.0-2.5%).					
31808500	2	3	theme	silk	351:354	arg1	SF					365:366	SF	365:366	SF	365:366	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	2	3	theme	silk	351:354	arg1	fibroin					356:362	silk fibroin	351:362	silk fibroin (SF) microspheres	351:380	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	1	4	theme	increased	240:248	arg1	risk					250:253	an increased risk	237:253	an increased risk for cardiovascular diseases in humans	237:291	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	0	5	theme	injectable	75:84	arg1	hydrogel					95:102	injectable alginate hydrogel	75:102	injectable alginate hydrogel	75:102	Sustained release of bioactive IGF-1 from a silk fibroin microsphere-based injectable alginate hydrogel for the treatment of myocardial infarction.					
31808500	6	6	link	enzyme-linked	1083:1095	arg1	results					1117:1123	The enzyme-linked immunosorbent assay results	1079:1123	The enzyme-linked immunosorbent assay results	1079:1123	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	4	7	theme	composite	840:848	arg1	hydrogel					861:868	the composite injectable hydrogel	836:868	the composite injectable hydrogel	836:868	Finally, this solution was crosslinked with 0.68% calcium gluconate solution to prepare the composite injectable hydrogel.					
31808500	7	8	theme	model	1575:1579	arg1	region					1556:1561	the peripheral region	1541:1561	the peripheral region of a MI rat model	1541:1579	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	6	9	from	hydrogels	1239:1247	arg1	release					1213:1219	the sustained release	1199:1219	the sustained release of IGF-1 from the hydrogels	1199:1247	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	6	10	theme	assay	1111:1115	arg1	results					1117:1123	The enzyme-linked immunosorbent assay results	1079:1123	The enzyme-linked immunosorbent assay results	1079:1123	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	3	11	theme	sodium	682:687	arg1	solutions					698:706	sodium alginate solutions	682:706	sodium alginate solutions	682:706	First, IGF-1 was physically adsorbed onto SF microspheres prepared by the coaxial needle system, and these IGF-1-containing microspheres were subsequently encapsulated into sodium alginate solutions at different concentrations (1.0-2.5%).					
31808500	6	12	theme	sustained	1301:1309	arg1	release					1311:1317	a better sustained release	1292:1317	a better sustained release ability	1292:1325	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	7	13	theme	hematoxylin-eosin	1400:1416	arg1	staining					1418:1425	hematoxylin-eosin staining	1400:1425	hematoxylin-eosin staining	1400:1425	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	8	14	theme	powerful	1720:1727	arg1	platform					1729:1736	a powerful platform	1718:1736	a powerful platform in cardiac tissue engineering	1718:1766	The applications of such a composite hydrogel may comprise a powerful platform in cardiac tissue engineering.					
31808500	7	15	dep	echocardiography	1382:1397	arg1	results					1458:1464	results	1458:1464	results	1458:1464	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	2	16	contain	containing	340:349	arg1	hydrogel					331:338	an injectable alginate hydrogel	308:338	an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1	308:432	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	2	16	contain	containing	340:349	arg2	microspheres					369:380	silk fibroin (SF) microspheres	351:380	silk fibroin (SF) microspheres	351:380	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	6	17	theme	better	1294:1299	arg1	release					1311:1317	a better sustained release	1292:1317	a better sustained release ability	1292:1325	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	5	18	theme	%	961:961	arg1	concentration					941:953	a concentration	939:953	a concentration of 1.5%	939:961	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5% could promote proliferation of H9C2 cardiomyocytes and reduce the cellular apoptosis rate under hypoxic conditions.					
31808500	8	19	theme	cardiac	1741:1747	arg1	engineering					1756:1766	cardiac tissue engineering	1741:1766	cardiac tissue engineering	1741:1766	The applications of such a composite hydrogel may comprise a powerful platform in cardiac tissue engineering.					
31808500	1	20	theme	cardiovascular	259:272	arg1	diseases					274:281	cardiovascular diseases	259:281	cardiovascular diseases in humans	259:291	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	7	21	theme	composite	1517:1525	arg1	hydrogel					1527:1534	the composite hydrogel	1513:1534	the composite hydrogel	1513:1534	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	6	22	theme	enzyme-linked	1083:1095	arg1	results					1117:1123	The enzyme-linked immunosorbent assay results	1079:1123	The enzyme-linked immunosorbent assay results	1079:1123	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	6	23	theme	sustained	1203:1211	arg1	release					1213:1219	the sustained release	1199:1219	the sustained release of IGF-1 from the hydrogels	1199:1247	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	1	24	theme	insulin-like	174:185	arg1	factor					194:199	insulin-like growth factor 1	174:201	insulin-like growth factor 1 (IGF-1)	174:209	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	1	24	theme	insulin-like	174:185	arg1	IGF-1					204:208	IGF-1	204:208	IGF-1	204:208	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	7	25	theme	infarct	1598:1604	arg1	size					1606:1609	the infarct size	1594:1609	the infarct size	1594:1609	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	6	26	theme	SF	1140:1141	arg1	microspheres					1143:1154	SF microspheres	1140:1154	SF microspheres as microcarriers	1140:1171	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	1	27	theme	growth	187:192	arg1	factor					194:199	insulin-like growth factor 1	174:201	insulin-like growth factor 1 (IGF-1)	174:209	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	1	27	theme	growth	187:192	arg1	IGF-1					204:208	IGF-1	204:208	IGF-1	204:208	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	2	28	theme	IGF-1	428:432	arg1	release					417:423	the release	413:423	the release of IGF-1	413:432	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	2	29	theme	myocardial	457:466	arg1	repair					468:473	myocardial repair	457:473	myocardial repair	457:473	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	1	30	from	diseases	274:281	arg1	humans					286:291	humans	286:291	humans	286:291	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	8	31	from	platform	1729:1736	arg1	engineering					1756:1766	cardiac tissue engineering	1741:1766	cardiac tissue engineering	1741:1766	The applications of such a composite hydrogel may comprise a powerful platform in cardiac tissue engineering.					
31808500	8	32	theme	tissue	1749:1754	arg1	engineering					1756:1766	cardiac tissue engineering	1741:1766	cardiac tissue engineering	1741:1766	The applications of such a composite hydrogel may comprise a powerful platform in cardiac tissue engineering.					
31808500	1	33	theme	factor	194:199	arg1	levels					164:169	Low circulating levels	148:169	Low circulating levels of insulin-like growth factor 1 (IGF-1)	148:209	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	0	34	theme	Sustained	0:8	arg1	release					10:16	Sustained release	0:16	Sustained release of bioactive IGF-1 from a silk fibroin	0:55	Sustained release of bioactive IGF-1 from a silk fibroin microsphere-based injectable alginate hydrogel for the treatment of myocardial infarction.					
31808500	4	35	theme	injectable	850:859	arg1	hydrogel					861:868	the composite injectable hydrogel	836:868	the composite injectable hydrogel	836:868	Finally, this solution was crosslinked with 0.68% calcium gluconate solution to prepare the composite injectable hydrogel.					
31808500	0	36	theme	bioactive	21:29	arg1	IGF-1					31:35	bioactive IGF-1	21:35	bioactive IGF-1	21:35	Sustained release of bioactive IGF-1 from a silk fibroin microsphere-based injectable alginate hydrogel for the treatment of myocardial infarction.					
31808500	5	37	theme	H9C2	994:997	arg1	cardiomyocytes					999:1012	H9C2 cardiomyocytes	994:1012	H9C2 cardiomyocytes	994:1012	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5% could promote proliferation of H9C2 cardiomyocytes and reduce the cellular apoptosis rate under hypoxic conditions.					
31808500	0	38	theme	myocardial	125:134	arg1	infarction					136:145	myocardial infarction	125:145	myocardial infarction	125:145	Sustained release of bioactive IGF-1 from a silk fibroin microsphere-based injectable alginate hydrogel for the treatment of myocardial infarction.					
31808500	5	39	theme	cellular	1029:1036	arg1	rate					1048:1051	the cellular apoptosis rate	1025:1051	the cellular apoptosis rate under hypoxic conditions	1025:1076	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5% could promote proliferation of H9C2 cardiomyocytes and reduce the cellular apoptosis rate under hypoxic conditions.					
31808500	6	40	dep	enzyme-linked	1083:1095	arg1	immunosorbent					1097:1109	immunosorbent	1097:1109	immunosorbent	1097:1109	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	6	41	theme	composite	1262:1270	arg1	hydrogel					1272:1279	the composite hydrogel	1258:1279	the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres	1258:1369	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	7	42	theme	trichrome	1439:1447	arg1	staining					1449:1456	Masson trichrome staining	1432:1456	Masson trichrome staining	1432:1456	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	5	43	theme	composite	875:883	arg1	hydrogel					885:892	The composite hydrogel	871:892	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5%	871:961	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5% could promote proliferation of H9C2 cardiomyocytes and reduce the cellular apoptosis rate under hypoxic conditions.					
31808500	5	44	theme	sodium	911:916	arg1	solution					927:934	a sodium alginate solution	909:934	a sodium alginate solution	909:934	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5% could promote proliferation of H9C2 cardiomyocytes and reduce the cellular apoptosis rate under hypoxic conditions.					
31808500	6	45	theme	SF	1355:1356	arg1	microspheres					1358:1369	the SF microspheres	1351:1369	the SF microspheres	1351:1369	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	3	46	theme	IGF-1-containing	616:631	arg1	microspheres					633:644	these IGF-1-containing microspheres	610:644	these IGF-1-containing microspheres	610:644	First, IGF-1 was physically adsorbed onto SF microspheres prepared by the coaxial needle system, and these IGF-1-containing microspheres were subsequently encapsulated into sodium alginate solutions at different concentrations (1.0-2.5%).					
31808500	4	47	theme	calcium	798:804	arg1	solution					816:823	0.68% calcium gluconate solution	792:823	0.68% calcium gluconate solution	792:823	Finally, this solution was crosslinked with 0.68% calcium gluconate solution to prepare the composite injectable hydrogel.					
31808500	6	48	theme	IGF-1	1224:1228	arg1	release					1213:1219	the sustained release	1199:1219	the sustained release of IGF-1 from the hydrogels	1199:1247	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	7	49	theme	intramyocardial	1484:1498	arg1	injection					1500:1508	an intramyocardial injection	1481:1508	an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model	1481:1579	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	0	50	theme	IGF-1	31:35	arg1	release					10:16	Sustained release	0:16	Sustained release of bioactive IGF-1 from a silk fibroin	0:55	Sustained release of bioactive IGF-1 from a silk fibroin microsphere-based injectable alginate hydrogel for the treatment of myocardial infarction.					
31808500	7	51	theme	hydrogel	1527:1534	arg1	injection					1500:1508	an intramyocardial injection	1481:1508	an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model	1481:1579	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	0	52	theme	infarction	136:145	arg1	treatment					112:120	the treatment	108:120	the treatment of myocardial infarction	108:145	Sustained release of bioactive IGF-1 from a silk fibroin microsphere-based injectable alginate hydrogel for the treatment of myocardial infarction.					
31808500	7	53	theme	rat	1571:1573	arg1	model					1575:1579	a MI rat model	1566:1579	a MI rat model	1566:1579	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	4	54	theme	%	796:796	arg1	solution					816:823	0.68% calcium gluconate solution	792:823	0.68% calcium gluconate solution	792:823	Finally, this solution was crosslinked with 0.68% calcium gluconate solution to prepare the composite injectable hydrogel.					
31808500	0	55	theme	silk	44:47	arg1	fibroin					49:55	a silk fibroin	42:55	a silk fibroin	42:55	Sustained release of bioactive IGF-1 from a silk fibroin microsphere-based injectable alginate hydrogel for the treatment of myocardial infarction.					
31808500	0	56	from	fibroin	49:55	arg1	release					10:16	Sustained release	0:16	Sustained release of bioactive IGF-1 from a silk fibroin	0:55	Sustained release of bioactive IGF-1 from a silk fibroin microsphere-based injectable alginate hydrogel for the treatment of myocardial infarction.					
31808500	3	57	theme	SF	551:552	arg1	microspheres					554:565	SF microspheres	551:565	SF microspheres prepared by the coaxial needle system	551:603	First, IGF-1 was physically adsorbed onto SF microspheres prepared by the coaxial needle system, and these IGF-1-containing microspheres were subsequently encapsulated into sodium alginate solutions at different concentrations (1.0-2.5%).					
31808500	5	58	theme	apoptosis	1038:1046	arg1	rate					1048:1051	the cellular apoptosis rate	1025:1051	the cellular apoptosis rate under hypoxic conditions	1025:1076	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5% could promote proliferation of H9C2 cardiomyocytes and reduce the cellular apoptosis rate under hypoxic conditions.					
31808500	4	59	theme	gluconate	806:814	arg1	solution					816:823	0.68% calcium gluconate solution	792:823	0.68% calcium gluconate solution	792:823	Finally, this solution was crosslinked with 0.68% calcium gluconate solution to prepare the composite injectable hydrogel.					
31808500	2	60	theme	alginate	322:329	arg1	hydrogel					331:338	an injectable alginate hydrogel	308:338	an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1	308:432	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	8	61	theme	composite	1686:1694	arg1	hydrogel					1696:1703	such a composite hydrogel	1679:1703	such a composite hydrogel	1679:1703	The applications of such a composite hydrogel may comprise a powerful platform in cardiac tissue engineering.					
31808500	5	62	theme	cardiomyocytes	999:1012	arg1	proliferation					977:989	proliferation	977:989	proliferation of H9C2 cardiomyocytes	977:1012	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5% could promote proliferation of H9C2 cardiomyocytes and reduce the cellular apoptosis rate under hypoxic conditions.					
31808500	6	63	theme	release	1311:1317	arg1	ability					1319:1325	a better sustained release ability	1292:1325	a better sustained release ability	1292:1325	The enzyme-linked immunosorbent assay results indicated that SF microspheres as microcarriers could effectively enhance the sustained release of IGF-1 from the hydrogels, causing the composite hydrogel to possess a better sustained release ability than the system without the SF microspheres.					
31808500	7	64	theme	MI	1568:1569	arg1	model					1575:1579	a MI rat model	1566:1579	a MI rat model	1566:1579	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	3	65	theme	needle	591:596	arg1	system					598:603	the coaxial needle system	579:603	the coaxial needle system	579:603	First, IGF-1 was physically adsorbed onto SF microspheres prepared by the coaxial needle system, and these IGF-1-containing microspheres were subsequently encapsulated into sodium alginate solutions at different concentrations (1.0-2.5%).					
31808500	2	66	theme	injectable	311:320	arg1	hydrogel					331:338	an injectable alginate hydrogel	308:338	an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1	308:432	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	7	67	theme	Masson	1432:1437	arg1	staining					1449:1456	Masson trichrome staining	1432:1456	Masson trichrome staining	1432:1456	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	7	68	theme	cardiac	1627:1633	arg1	function					1635:1642	the cardiac function	1623:1642	the cardiac function	1623:1642	Moreover, echocardiography, hematoxylin-eosin staining, and Masson trichrome staining results indicated that an intramyocardial injection of the composite hydrogel into the peripheral region of a MI rat model could reduce the infarct size and improve the cardiac function after 28 days.					
31808500	1	69	theme	circulating	152:162	arg1	levels					164:169	Low circulating levels	148:169	Low circulating levels of insulin-like growth factor 1 (IGF-1)	148:209	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	5	70	theme	alginate	918:925	arg1	solution					927:934	a sodium alginate solution	909:934	a sodium alginate solution	909:934	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5% could promote proliferation of H9C2 cardiomyocytes and reduce the cellular apoptosis rate under hypoxic conditions.					
31808500	2	71	theme	myocardial	481:490	arg1	MI					504:505	MI	504:505	MI	504:505	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	2	71	theme	myocardial	481:490	arg1	infarction					492:501	myocardial infarction	481:501	myocardial infarction (MI)	481:506	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
31808500	5	72	theme	hypoxic	1059:1065	arg1	conditions					1067:1076	hypoxic conditions	1059:1076	hypoxic conditions	1059:1076	The composite hydrogel prepared using a sodium alginate solution at a concentration of 1.5% could promote proliferation of H9C2 cardiomyocytes and reduce the cellular apoptosis rate under hypoxic conditions.					
31808500	4	73	theme	0.68	792:795	arg1	%					796:796	%	796:796	%	796:796	Finally, this solution was crosslinked with 0.68% calcium gluconate solution to prepare the composite injectable hydrogel.					
31808500	1	74	theme	Low	148:150	arg1	levels					164:169	Low circulating levels	148:169	Low circulating levels of insulin-like growth factor 1 (IGF-1)	148:209	Low circulating levels of insulin-like growth factor 1 (IGF-1) have been correlated with an increased risk for cardiovascular diseases in humans.					
31808500	8	75	theme	hydrogel	1696:1703	arg1	applications					1663:1674	The applications	1659:1674	The applications of such a composite hydrogel	1659:1703	The applications of such a composite hydrogel may comprise a powerful platform in cardiac tissue engineering.					
31808500	3	76	theme	alginate	689:696	arg1	solutions					698:706	sodium alginate solutions	682:706	sodium alginate solutions	682:706	First, IGF-1 was physically adsorbed onto SF microspheres prepared by the coaxial needle system, and these IGF-1-containing microspheres were subsequently encapsulated into sodium alginate solutions at different concentrations (1.0-2.5%).					
31808500	3	77	theme	coaxial	583:589	arg1	system					598:603	the coaxial needle system	579:603	the coaxial needle system	579:603	First, IGF-1 was physically adsorbed onto SF microspheres prepared by the coaxial needle system, and these IGF-1-containing microspheres were subsequently encapsulated into sodium alginate solutions at different concentrations (1.0-2.5%).					
31808500	2	78	theme	fibroin	356:362	arg1	microspheres					369:380	silk fibroin (SF) microspheres	351:380	silk fibroin (SF) microspheres	351:380	In this work, an injectable alginate hydrogel containing silk fibroin (SF) microspheres with the capability to sustain the release of IGF-1 was prepared to induce myocardial repair after myocardial infarction (MI).					
29853380	2	0	theme	chitinases	448:457	arg1	removal					437:443	A significant removal	423:443	A significant removal of chitinases	423:457	A significant removal of chitinases was observed after fining an aromatic white wine with 1 g/L of a fungoid chitosan.					
29853380	5	1	theme	1 g/L	1038:1042	arg1	chitosan					1044:1051	a 1 g/L chitosan	1036:1051	a 1 g/L chitosan addition	1036:1060	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
29853380	4	2	theme	acid	805:808	arg1	reduction					783:791	a reduction	781:791	a reduction of tartaric acid, malic acid, potassium and iron	781:840	Among the secondary effects of a fining treatment, a reduction of tartaric acid, malic acid, potassium and iron was showed.					
29853380	3	3	theme	chitosan	664:671	arg1	samples					678:684	the fined chitosan wine samples	654:684	the fined chitosan wine samples	654:684	Even if the more stable thaumatin like protein fractions were not significantly affected, the heat stability of the fined chitosan wine samples were highly improved in the 55-62 °C range.					
29853380	1	4	from	effects	371:377	arg1	compounds					412:420	untargeted fixed and volatile compounds	382:420	untargeted fixed and volatile compounds	382:420	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	3	5	theme	heat	636:639	arg1	stability					641:649	the heat stability	632:649	the heat stability of the fined chitosan wine samples	632:684	Even if the more stable thaumatin like protein fractions were not significantly affected, the heat stability of the fined chitosan wine samples were highly improved in the 55-62 °C range.					
29853380	0	6	theme	volatile	129:136	arg1	compounds					138:146	volatile compounds	129:146	volatile compounds	129:146	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	1	7	theme	fixed	393:397	arg1	compounds					412:420	untargeted fixed and volatile compounds	382:420	untargeted fixed and volatile compounds	382:420	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	2	8	theme	aromatic	488:495	arg1	wine					503:506	an aromatic white wine	485:506	an aromatic white wine with 1 g/L of a fungoid chitosan	485:539	A significant removal of chitinases was observed after fining an aromatic white wine with 1 g/L of a fungoid chitosan.					
29853380	4	9	theme	malic	811:815	arg1	acid					817:820	malic acid	811:820	malic acid	811:820	Among the secondary effects of a fining treatment, a reduction of tartaric acid, malic acid, potassium and iron was showed.					
29853380	3	10	theme	wine	673:676	arg1	samples					678:684	the fined chitosan wine samples	654:684	the fined chitosan wine samples	654:684	Even if the more stable thaumatin like protein fractions were not significantly affected, the heat stability of the fined chitosan wine samples were highly improved in the 55-62 °C range.					
29853380	1	11	theme	volatile	403:410	arg1	compounds					412:420	untargeted fixed and volatile compounds	382:420	untargeted fixed and volatile compounds	382:420	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	1	12	theme	chitosan	216:223	arg1	treatment					225:233	chitosan treatment	216:233	chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds	216:420	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	1	13	theme	wine	308:311	arg1	potential					318:326	the wine haze potential	304:326	the wine haze potential	304:326	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	0	14	theme	white	163:167	arg1	wine					169:172	an aromatic white wine	151:172	an aromatic white wine	151:172	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	1	15	theme	haze	313:316	arg1	potential					318:326	the wine haze potential	304:326	the wine haze potential	304:326	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	0	16	theme	aromatic	154:161	arg1	wine					169:172	an aromatic white wine	151:172	an aromatic white wine	151:172	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	5	17	with	terpenols	867:875	arg1	exception					886:894	the exception	882:894	the exception of α-terpineol	882:909	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
29853380	1	18	theme	potential	318:326	arg1	modification					288:299	any modification	284:299	any modification of the wine haze potential	284:326	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	3	19	theme	samples	678:684	arg1	stability					641:649	the heat stability	632:649	the heat stability of the fined chitosan wine samples	632:684	Even if the more stable thaumatin like protein fractions were not significantly affected, the heat stability of the fined chitosan wine samples were highly improved in the 55-62 °C range.					
29853380	5	20	theme	chitosan	1044:1051	arg1	addition					1053:1060	a 1 g/L chitosan addition	1036:1060	a 1 g/L chitosan addition	1036:1060	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
29853380	1	21	theme	treatment	225:233	arg1	use					209:211	the use	205:211	the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds	205:420	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	0	22	from	Effects	65:71	arg1	heat-stability					76:89	heat-stability	76:89	heat-stability	76:89	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	0	22	from	Effects	65:71	arg1	colour					117:122	colour	117:122	colour	117:122	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	0	22	from	Effects	65:71	arg1	proteins					92:99	proteins	92:99	proteins	92:99	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	0	22	from	Effects	65:71	arg1	wine					169:172	an aromatic white wine	151:172	an aromatic white wine	151:172	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	0	22	from	Effects	65:71	arg1	acids					110:114	organic acids	102:114	organic acids	102:114	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	0	22	from	Effects	65:71	arg1	compounds					138:146	volatile compounds	129:146	volatile compounds	129:146	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	4	23	theme	tartaric	796:803	arg1	acid					805:808	tartaric acid	796:808	tartaric acid	796:808	Among the secondary effects of a fining treatment, a reduction of tartaric acid, malic acid, potassium and iron was showed.					
29853380	0	24	theme	chitosan	11:18	arg1	use					4:6	The use	0:6	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.	0:173	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	5	25	theme	free	862:865	arg1	terpenols					867:875	All the free terpenols	854:875	All the free terpenols with the exception of α-terpineol	854:909	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
29853380	4	26	theme	secondary	740:748	arg1	effects					750:756	the secondary effects	736:756	the secondary effects of a fining treatment	736:778	Among the secondary effects of a fining treatment, a reduction of tartaric acid, malic acid, potassium and iron was showed.					
29853380	4	27	theme	iron	837:840	arg1	reduction					783:791	a reduction	781:791	a reduction of tartaric acid, malic acid, potassium and iron	781:840	Among the secondary effects of a fining treatment, a reduction of tartaric acid, malic acid, potassium and iron was showed.					
29853380	1	28	theme	bentonite	248:256	arg1	fining					258:263	a bentonite fining	246:263	a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds	246:420	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	5	29	theme	fermentative	986:997	arg1	compounds					1005:1013	the fermentative aroma compounds	982:1013	the fermentative aroma compounds	982:1013	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
29853380	5	30	theme	significant	927:937	arg1	amounts					939:945	significant amounts	927:945	significant amounts	927:945	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
29853380	2	31	theme	significant	425:435	arg1	removal					437:443	A significant removal	423:443	A significant removal of chitinases	423:457	A significant removal of chitinases was observed after fining an aromatic white wine with 1 g/L of a fungoid chitosan.					
29853380	0	32	theme	organic	102:108	arg1	acids					110:114	organic acids	102:114	organic acids	102:114	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	3	33	theme	protein	581:587	arg1	fractions					589:597	protein fractions	581:597	protein fractions	581:597	Even if the more stable thaumatin like protein fractions were not significantly affected, the heat stability of the fined chitosan wine samples were highly improved in the 55-62 °C range.					
29853380	2	34	theme	chitosan	532:539	arg1	1 g/L					513:517	1 g/L	513:517	1 g/L of a fungoid chitosan	513:539	A significant removal of chitinases was observed after fining an aromatic white wine with 1 g/L of a fungoid chitosan.					
29853380	4	35	theme	treatment	770:778	arg1	effects					750:756	the secondary effects	736:756	the secondary effects of a fining treatment	736:778	Among the secondary effects of a fining treatment, a reduction of tartaric acid, malic acid, potassium and iron was showed.					
29853380	3	36	theme	fined	658:662	arg1	samples					678:684	the fined chitosan wine samples	654:684	the fined chitosan wine samples	654:684	Even if the more stable thaumatin like protein fractions were not significantly affected, the heat stability of the fined chitosan wine samples were highly improved in the 55-62 °C range.					
29853380	2	37	theme	white	497:501	arg1	wine					503:506	an aromatic white wine	485:506	an aromatic white wine with 1 g/L of a fungoid chitosan	485:539	A significant removal of chitinases was observed after fining an aromatic white wine with 1 g/L of a fungoid chitosan.					
29853380	2	38	theme	fungoid	524:530	arg1	chitosan					532:539	a fungoid chitosan	522:539	a fungoid chitosan	522:539	A significant removal of chitinases was observed after fining an aromatic white wine with 1 g/L of a fungoid chitosan.					
29853380	1	39	theme	secondary	361:369	arg1	effects					371:377	the secondary effects	357:377	the secondary effects on untargeted fixed and volatile compounds	357:420	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	4	40	theme	fining	763:768	arg1	treatment					770:778	a fining treatment	761:778	a fining treatment	761:778	Among the secondary effects of a fining treatment, a reduction of tartaric acid, malic acid, potassium and iron was showed.					
29853380	5	41	theme	aroma	999:1003	arg1	compounds					1005:1013	the fermentative aroma compounds	982:1013	the fermentative aroma compounds	982:1013	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
29853380	2	42	with	wine	503:506	arg1	1 g/L					513:517	1 g/L	513:517	1 g/L of a fungoid chitosan	513:539	A significant removal of chitinases was observed after fining an aromatic white wine with 1 g/L of a fungoid chitosan.					
29853380	3	43	theme	55-62 °C	714:721	arg1	range					723:727	the 55-62 °C range	710:727	the 55-62 °C range	710:727	Even if the more stable thaumatin like protein fractions were not significantly affected, the heat stability of the fined chitosan wine samples were highly improved in the 55-62 °C range.					
29853380	5	44	theme	glycosylated	959:970	arg1	forms					972:976	the glycosylated forms	955:976	the glycosylated forms	955:976	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
29853380	5	45	theme	α-terpineol	899:909	arg1	exception					886:894	the exception	882:894	the exception of α-terpineol	882:909	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
29853380	4	46	theme	potassium	823:831	arg1	reduction					783:791	a reduction	781:791	a reduction of tartaric acid, malic acid, potassium and iron	781:840	Among the secondary effects of a fining treatment, a reduction of tartaric acid, malic acid, potassium and iron was showed.					
29853380	0	47	theme	wine	52:55	arg1	fining					57:62	wine fining	52:62	wine fining	52:62	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	1	48	theme	untargeted	382:391	arg1	compounds					412:420	untargeted fixed and volatile compounds	382:420	untargeted fixed and volatile compounds	382:420	This investigation focuses on the use of chitosan treatment simulating a bentonite fining in order to detect any modification of the wine haze potential by simultaneously evaluating the secondary effects on untargeted fixed and volatile compounds.					
29853380	0	49	dep	use	4:6	arg1	Effects					65:71	Effects	65:71	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.	0:173	The use of chitosan as alternative to bentonite for wine fining: Effects on heat-stability, proteins, organic acids, colour, and volatile compounds in an aromatic white wine.					
29853380	4	50	theme	acid	817:820	arg1	reduction					783:791	a reduction	781:791	a reduction of tartaric acid, malic acid, potassium and iron	781:840	Among the secondary effects of a fining treatment, a reduction of tartaric acid, malic acid, potassium and iron was showed.					
29853380	3	51	theme	stable	559:564	arg1	thaumatin					566:574	the more stable thaumatin	550:574	the more stable thaumatin like protein fractions	550:597	Even if the more stable thaumatin like protein fractions were not significantly affected, the heat stability of the fined chitosan wine samples were highly improved in the 55-62 °C range.					
29853380	5	52	gly	glycosylated	959:970	arg1	forms					972:976	the glycosylated forms	955:976	the glycosylated forms	955:976	All the free terpenols with the exception of α-terpineol were reduced in significant amounts, whilst the glycosylated forms and the fermentative aroma compounds were not affected by a 1 g/L chitosan addition.					
30440309	7	0	theme	flexible	1280:1287	arg1	bioelectrodes					1300:1312	flexible conductive bioelectrodes	1280:1312	flexible conductive bioelectrodes	1280:1312	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	4	1	from	power	810:814	arg1	range					833:837	the microwatt range	819:837	the microwatt range	819:837	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	7	2	theme	cell	1353:1356	arg1	system					1358:1363	glucose biofuel cell system	1337:1363	glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme	1337:1440	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	6	3	theme	physiological	1032:1044	arg1	concentration					1054:1066	physiological glucose concentration	1032:1066	physiological glucose concentration	1032:1066	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	1	4	from	network	143:149	arg1	film					185:188	20 μm nanostructured cellulose film	154:188	20 μm nanostructured cellulose film	154:188	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	4	5	theme	glucose	716:722	arg1	energy					706:711	the biochemical energy	690:711	the biochemical energy of glucose	690:722	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	2	6	with	pellicle	297:304	arg1	MWCNTs					342:347	MWCNTs	342:347	MWCNTs	342:347	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	2	6	with	pellicle	297:304	arg1	nanotubes					331:339	multi-walled carbon nanotubes	311:339	multi-walled carbon nanotubes (MWCNTs)	311:348	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	3	7	theme	composite	355:363	arg1	film					365:368	The composite film	351:368	The composite film	351:368	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	5	8	theme	μA/cm2	988:993	arg1	density					973:979	a current density	963:979	a current density of 381 μA/cm2 in the presence of 25 mM glucose	963:1026	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	6	9	theme	mV	1145:1146	arg1	voltage					1112:1118	an open circuit voltage and power density	1096:1136	voltage	1112:1118	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	6	9	theme	mV	1145:1146	arg1	density					1130:1136	an open circuit voltage and power density	1096:1136	density	1130:1136	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	6	9	theme	mV	1145:1146	arg1	μW/cm2					1159:1164	24.975 μW/cm2	1152:1164	24.975 μW/cm2	1152:1164	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	2	10	theme	nanocellulose	283:295	arg1	pellicle					297:304	bacterial nanocellulose pellicle	273:304	bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs)	273:348	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	6	11	theme	power	1124:1128	arg1	density					1130:1136	an open circuit voltage and power density	1096:1136	density	1130:1136	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	7	12	theme	bacterial	1383:1391	arg1	nanocellulose					1393:1405	synthesized bacterial nanocellulose	1371:1405	synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme	1371:1440	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	1	13	theme	nanotube	68:75	arg1	pellicle					93:100	Carbon nanotube (CNT)-cellulose pellicle	61:100	Carbon nanotube (CNT)-cellulose pellicle	61:100	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	2	14	theme	bacterial	273:281	arg1	pellicle					297:304	bacterial nanocellulose pellicle	273:304	bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs)	273:348	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	6	15	theme	open	1099:1102	arg1	voltage					1112:1118	an open circuit voltage and power density	1096:1136	voltage	1112:1118	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	7	16	theme	system	1358:1363	arg1	development					1322:1332	the development	1318:1332	the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme	1318:1440	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	4	17	theme	based	643:647	arg1	system					673:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	4	18	theme	electrical	799:808	arg1	power					810:814	electrical power	799:814	electrical power in the microwatt range	799:837	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	3	19	theme	quinone	434:440	arg1	dehydrogenase					450:462	pyroquinoline quinone glucose dehydrogenase	420:462	pyroquinoline quinone glucose dehydrogenase (PQQ-GDH)	420:472	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	3	19	theme	quinone	434:440	arg1	PQQ-GDH					465:471	PQQ-GDH	465:471	PQQ-GDH	465:471	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	5	20	theme	open	877:880	arg1	voltage					890:896	an open circuit voltage and power density	874:914	voltage	890:896	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	3	21	mod	modified	382:389	arg3	oxidase					488:494	bilirubin oxidase	478:494	bilirubin oxidase (BODx)	478:501	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	3	21	mod	modified	382:389	arg3	dehydrogenase					450:462	pyroquinoline quinone glucose dehydrogenase	420:462	pyroquinoline quinone glucose dehydrogenase (PQQ-GDH)	420:472	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	3	21	mod	modified	382:389	arg3	enzymes					402:408	redox enzymes	396:408	redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source	396:598	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	3	21	mod	modified	382:389	arg1	film					365:368	The composite film	351:368	The composite film	351:368	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	1	22	theme	CNT	78:80	arg1	pellicle					93:100	Carbon nanotube (CNT)-cellulose pellicle	61:100	Carbon nanotube (CNT)-cellulose pellicle	61:100	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	6	23	theme	biofuel	1073:1079	arg1	cell					1081:1084	the biofuel cell	1069:1084	the biofuel cell	1069:1084	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	1	24	theme	μm	157:158	arg1	film					185:188	20 μm nanostructured cellulose film	154:188	20 μm nanostructured cellulose film	154:188	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	0	25	theme	Nanotube-Cellulose	7:24	arg1	Pellicle					26:33	Carbon Nanotube-Cellulose Pellicle	0:33	Carbon Nanotube-Cellulose Pellicle for Glucose	0:45	Carbon Nanotube-Cellulose Pellicle for Glucose Biofuel Cell.					
30440309	7	26	theme	diverse	1469:1475	arg1	applications					1477:1488	diverse applications	1469:1488	diverse applications in enzymatic biofuel cell and biosensor technology	1469:1539	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	2	27	theme	carbon	324:329	arg1	MWCNTs					342:347	MWCNTs	342:347	MWCNTs	342:347	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	2	27	theme	carbon	324:329	arg1	nanotubes					331:339	multi-walled carbon nanotubes	311:339	multi-walled carbon nanotubes (MWCNTs)	311:348	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	5	28	from	μA/cm2	988:993	arg1	presence					1002:1009	the presence	998:1009	the presence of 25 mM glucose	998:1026	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	1	29	theme	nanostructured	160:173	arg1	film					185:188	20 μm nanostructured cellulose film	154:188	20 μm nanostructured cellulose film	154:188	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	0	30	theme	Carbon	0:5	arg1	Pellicle					26:33	Carbon Nanotube-Cellulose Pellicle	0:33	Carbon Nanotube-Cellulose Pellicle for Glucose	0:45	Carbon Nanotube-Cellulose Pellicle for Glucose Biofuel Cell.					
30440309	7	31	theme	enzymatic	1493:1501	arg1	cell					1511:1514	enzymatic biofuel cell	1493:1514	enzymatic biofuel cell	1493:1514	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	1	32	theme	-cellulose	82:91	arg1	pellicle					93:100	Carbon nanotube (CNT)-cellulose pellicle	61:100	Carbon nanotube (CNT)-cellulose pellicle	61:100	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	1	33	theme	cellulose	175:183	arg1	film					185:188	20 μm nanostructured cellulose film	154:188	20 μm nanostructured cellulose film	154:188	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	7	34	theme	glucose	1337:1343	arg1	system					1358:1363	glucose biofuel cell system	1337:1363	glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme	1337:1440	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	7	35	dep	strategy	1260:1267	arg1	prepare					1272:1278	prepare	1272:1278	to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme	1269:1440	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	5	36	theme	mM	1017:1018	arg1	glucose					1020:1026	25 mM glucose	1014:1026	25 mM glucose	1014:1026	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	4	37	theme	molecular	770:778	arg1	oxygen					780:785	molecular oxygen	770:785	molecular oxygen	770:785	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	6	38	theme	293.75μA/cm2	1206:1217	arg1	density					1195:1201	a current density	1185:1201	a current density of 293.75μA/cm2	1185:1217	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	4	39	theme	glucose/O2	649:658	arg1	system					673:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	2	40	theme	multi-walled	311:322	arg1	MWCNTs					342:347	MWCNTs	342:347	MWCNTs	342:347	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	2	40	theme	multi-walled	311:322	arg1	nanotubes					331:339	multi-walled carbon nanotubes	311:339	multi-walled carbon nanotubes (MWCNTs)	311:348	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	2	41	theme	pellicle	297:304	arg1	modification					257:268	the modification	253:268	the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs)	253:348	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	5	42	from	presence	1002:1009	arg1	density					973:979	a current density	963:979	a current density of 381 μA/cm2 in the presence of 25 mM glucose	963:1026	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	3	43	theme	bilirubin	478:486	arg1	BODx					497:500	BODx	497:500	BODx	497:500	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	3	43	theme	bilirubin	478:486	arg1	oxidase					488:494	bilirubin oxidase	478:494	bilirubin oxidase (BODx)	478:501	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	4	44	theme	biochemical	694:704	arg1	energy					706:711	the biochemical energy	690:711	the biochemical energy of glucose	690:722	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	5	45	theme	glucose	1020:1026	arg1	presence					1002:1009	the presence	998:1009	the presence of 25 mM glucose	998:1026	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	3	46	theme	glucose	442:448	arg1	dehydrogenase					450:462	pyroquinoline quinone glucose dehydrogenase	420:462	pyroquinoline quinone glucose dehydrogenase (PQQ-GDH)	420:472	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	3	46	theme	glucose	442:448	arg1	PQQ-GDH					465:471	PQQ-GDH	465:471	PQQ-GDH	465:471	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	4	47	theme	cell	668:671	arg1	system					673:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	3	48	theme	pyroquinoline	420:432	arg1	dehydrogenase					450:462	pyroquinoline quinone glucose dehydrogenase	420:462	pyroquinoline quinone glucose dehydrogenase (PQQ-GDH)	420:472	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	3	48	theme	pyroquinoline	420:432	arg1	PQQ-GDH					465:471	PQQ-GDH	465:471	PQQ-GDH	465:471	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	4	49	theme	oxygen	780:785	arg1	oxidation					732:740	the oxidation	728:740	the oxidation of glucose	728:751	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	4	49	theme	oxygen	780:785	arg1	reduction					757:765	reduction	757:765	reduction	757:765	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	0	50	dep	Cell	55:58	arg1	Pellicle					26:33	Carbon Nanotube-Cellulose Pellicle	0:33	Carbon Nanotube-Cellulose Pellicle for Glucose	0:45	Carbon Nanotube-Cellulose Pellicle for Glucose Biofuel Cell.					
30440309	7	51	theme	biofuel	1345:1351	arg1	system					1358:1363	glucose biofuel cell system	1337:1363	glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme	1337:1440	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	4	52	theme	biofuel	660:666	arg1	system					673:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	3	53	theme	redox	396:400	arg1	dehydrogenase					450:462	pyroquinoline quinone glucose dehydrogenase	420:462	pyroquinoline quinone glucose dehydrogenase (PQQ-GDH)	420:472	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	3	53	theme	redox	396:400	arg1	enzymes					402:408	redox enzymes	396:408	redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source	396:598	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	3	53	theme	redox	396:400	arg1	oxidase					488:494	bilirubin oxidase	478:494	bilirubin oxidase (BODx)	478:501	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	5	54	theme	power	902:906	arg1	density					908:914	an open circuit voltage and power density	874:914	density	908:914	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	7	55	theme	biosensor	1520:1528	arg1	technology					1530:1539	biosensor technology	1520:1539	biosensor technology	1520:1539	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	5	56	theme	25	1014:1015	arg1	mM					1017:1018	mM	1017:1018	mM	1017:1018	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	5	57	theme	mV	923:924	arg1	voltage					890:896	an open circuit voltage and power density	874:914	voltage	890:896	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	5	57	theme	mV	923:924	arg1	density					908:914	an open circuit voltage and power density	874:914	density	908:914	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	5	57	theme	mV	923:924	arg1	μW/cm2					936:941	46.25 μW/cm2	930:941	46.25 μW/cm2	930:941	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	7	58	from	applications	1477:1488	arg1	cell					1511:1514	enzymatic biofuel cell	1493:1514	enzymatic biofuel cell	1493:1514	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	7	58	from	applications	1477:1488	arg1	technology					1530:1539	biosensor technology	1520:1539	biosensor technology	1520:1539	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	6	59	theme	glucose	1046:1052	arg1	concentration					1054:1066	physiological glucose concentration	1032:1066	physiological glucose concentration	1032:1066	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	2	60	theme	flexible	195:202	arg1	film					232:235	The flexible and electrically conductive film	191:235	The flexible and electrically conductive film	191:235	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	3	61	theme	biofuel	585:591	arg1	source					593:598	the biofuel source	581:598	the biofuel source	581:598	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	6	62	theme	current	1187:1193	arg1	density					1195:1201	a current density	1185:1201	a current density of 293.75μA/cm2	1185:1217	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	7	63	theme	conductive	1289:1298	arg1	bioelectrodes					1300:1312	flexible conductive bioelectrodes	1280:1312	flexible conductive bioelectrodes	1280:1312	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	7	64	theme	biofuel	1503:1509	arg1	cell					1511:1514	enzymatic biofuel cell	1493:1514	enzymatic biofuel cell	1493:1514	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	2	65	theme	conductive	221:230	arg1	film					232:235	The flexible and electrically conductive film	191:235	The flexible and electrically conductive film	191:235	The flexible and electrically conductive film was prepared by the modification of bacterial nanocellulose pellicle with multi-walled carbon nanotubes (MWCNTs).					
30440309	4	66	theme	glucose	745:751	arg1	oxidation					732:740	the oxidation	728:740	the oxidation of glucose	728:751	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	4	66	theme	glucose	745:751	arg1	reduction					757:765	reduction	757:765	reduction	757:765	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	4	67	theme	enzyme	605:610	arg1	system					673:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	3	68	theme	cathodic	533:540	arg1	catalyst					542:549	the anodic and cathodic catalyst	518:549	the anodic and cathodic catalyst	518:549	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	1	69	theme	Carbon	61:66	arg1	pellicle					93:100	Carbon nanotube (CNT)-cellulose pellicle	61:100	Carbon nanotube (CNT)-cellulose pellicle	61:100	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	3	70	theme	anodic	522:527	arg1	catalyst					542:549	the anodic and cathodic catalyst	518:549	the anodic and cathodic catalyst	518:549	The composite film was further modified with redox enzymes including pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BODx) functioning as the anodic and cathodic catalyst, respectively with glucose as the biofuel source.					
30440309	7	71	theme	synthesized	1371:1381	arg1	nanocellulose					1393:1405	synthesized bacterial nanocellulose	1371:1405	synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme	1371:1440	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	6	72	theme	circuit	1104:1110	arg1	voltage					1112:1118	an open circuit voltage and power density	1096:1136	voltage	1112:1118	At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 418 mV and 24.975 μW/cm2 respectively, with a current density of 293.75μA/cm2.					
30440309	7	73	theme	facile	1253:1258	arg1	strategy					1260:1267	this facile strategy	1248:1267	this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme	1248:1440	As a result, we expect that this facile strategy to prepare flexible conductive bioelectrodes for the development of glucose biofuel cell system using synthesized bacterial nanocellulose crosslinked with MWCNTs and enzyme can be readily extended to diverse applications in enzymatic biofuel cell and biosensor technology.					
30440309	4	74	theme	microwatt	823:831	arg1	range					833:837	the microwatt range	819:837	the microwatt range	819:837	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	5	75	theme	cell	852:855	arg1	system					857:862	The biofuel cell system	840:862	The biofuel cell system	840:862	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	1	76	theme	conductive	128:137	arg1	network					143:149	a conductive CNT network	126:149	a conductive CNT network on 20 μm nanostructured cellulose film	126:188	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	5	77	theme	circuit	882:888	arg1	voltage					890:896	an open circuit voltage and power density	874:914	voltage	890:896	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	4	78	theme	MWCNT-cellulose	627:641	arg1	system					673:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
30440309	5	79	theme	current	965:971	arg1	density					973:979	a current density	963:979	a current density of 381 μA/cm2 in the presence of 25 mM glucose	963:1026	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	5	80	theme	biofuel	844:850	arg1	system					857:862	The biofuel cell system	840:862	The biofuel cell system	840:862	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	1	81	theme	CNT	139:141	arg1	network					143:149	a conductive CNT network	126:149	a conductive CNT network on 20 μm nanostructured cellulose film	126:188	Carbon nanotube (CNT)-cellulose pellicle was developed to create a conductive CNT network on 20 μm nanostructured cellulose film.					
30440309	5	82	from	density	973:979	arg1	presence					1002:1009	the presence	998:1009	the presence of 25 mM glucose	998:1026	The biofuel cell system exhibited an open circuit voltage and power density of 470 mV and 46.25 μW/cm2, respectively, with a current density of 381 μA/cm2 in the presence of 25 mM glucose.					
30440309	4	83	theme	functionalized	612:625	arg1	system					673:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system	601:678	The enzyme functionalized MWCNT-cellulose based glucose/O2 biofuel cell system harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power in the microwatt range.					
29574641	0	0	theme	Cd	90:91	arg1	adsorption					76:85	adsorption	76:85	adsorption of Cd(II) and Pb(II)	76:106	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).					
29574641	2	1	theme	X-ray	459:463	arg1	spectroscopy					479:490	X-ray photoelectron spectroscopy	459:490	X-ray photoelectron spectroscopy	459:490	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	1	theme	X-ray	459:463	arg1	spectra					387:393	infrared spectra	378:393	infrared spectra	378:393	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	7	2	from	wastewater	1253:1262	arg1	removal					1205:1211	the removal	1201:1211	the removal of heavy metal ions from metal smelting wastewater	1201:1262	Studies were also conducted to demonstrate the applicability of the sorbent to the removal of heavy metal ions from metal smelting wastewater.					
29574641	1	3	theme	novel	111:115	arg1	nanocomposite					185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite	109:197	nanocomposite	185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	1	4	theme	graphene	318:325	arg1	oxide					327:331	graphene oxide	318:331	graphene oxide (GO) in an aqueous system	318:357	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	1	5	theme	adsorbent	117:125	arg1	nanocomposite					185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite	109:197	nanocomposite	185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	1	6	from	system	352:357	arg1	presence					306:313	the presence	302:313	the presence of graphene oxide (GO) in an aqueous system	302:357	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	1	7	theme	oxide	327:331	arg1	presence					306:313	the presence	302:313	the presence of graphene oxide (GO) in an aqueous system	302:357	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	2	8	theme	nanocomposite	584:596	arg1	structure					541:549	structure	541:549	structure	541:549	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	8	theme	nanocomposite	584:596	arg1	morphology					526:535	morphology	526:535	morphology	526:535	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	6	9	theme	kinetic	1107:1113	arg1	model					1115:1119	the pseudo-second-order kinetic model	1083:1119	the pseudo-second-order kinetic model	1083:1119	Meanwhile, the adsorption process matched well with the Langmuir isotherm model and the pseudo-second-order kinetic model.					
29574641	3	10	theme	Pb	660:661	arg1	cleanup					638:644	cleanup	638:644	cleanup of Cd(II) and Pb(II)	638:665	A series of adsorption experiments for cleanup of Cd(II) and Pb(II) were conducted to investigate the effects of lignin and sodium alginate on the graphene structure.					
29574641	2	11	theme	PG/L/SA	576:582	arg1	nanocomposite					584:596	this novel functional PG/L/SA nanocomposite	554:596	this novel functional PG/L/SA nanocomposite	554:596	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	5	12	theme	adsorption	917:926	arg1	capacity					928:935	the adsorption capacity	913:935	the adsorption capacity for Cd(II) and Pb(II) of 79.88 and 226.24 mg/g	913:982	The as-prepared material achieved the adsorption capacity for Cd(II) and Pb(II) of 79.88 and 226.24 mg/g, respectively.					
29574641	1	13	theme	hydrothermal	241:252	arg1	polymerization					254:267	hydrothermal polymerization	241:267	hydrothermal polymerization of lignin and sodium alginate	241:297	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	3	14	theme	alginate	730:737	arg1	effects					701:707	the effects	697:707	the effects of lignin and sodium alginate on the graphene structure	697:763	A series of adsorption experiments for cleanup of Cd(II) and Pb(II) were conducted to investigate the effects of lignin and sodium alginate on the graphene structure.					
29574641	7	15	theme	ions	1228:1231	arg1	removal					1205:1211	the removal	1201:1211	the removal of heavy metal ions from metal smelting wastewater	1201:1262	Studies were also conducted to demonstrate the applicability of the sorbent to the removal of heavy metal ions from metal smelting wastewater.					
29574641	7	16	theme	smelting	1244:1251	arg1	wastewater					1253:1262	metal smelting wastewater	1238:1262	metal smelting wastewater	1238:1262	Studies were also conducted to demonstrate the applicability of the sorbent to the removal of heavy metal ions from metal smelting wastewater.					
29574641	2	17	theme	scanning	425:432	arg1	microscopy					443:452	scanning electron microscopy	425:452	scanning electron microscopy	425:452	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	17	theme	scanning	425:432	arg1	spectra					387:393	infrared spectra	378:393	infrared spectra	378:393	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	3	18	from	effects	701:707	arg1	structure					755:763	the graphene structure	742:763	the graphene structure	742:763	A series of adsorption experiments for cleanup of Cd(II) and Pb(II) were conducted to investigate the effects of lignin and sodium alginate on the graphene structure.					
29574641	4	19	theme	capacity	836:843	arg1	contrast					845:852	adsorption capacity contrast	825:852	adsorption capacity contrast to porous graphene (PG)	825:876	It was found that PG/L/SA showed a significant increase in adsorption capacity contrast to porous graphene (PG).					
29574641	1	20	theme	three-dimensional	128:144	arg1	nanocomposite					185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite	109:197	nanocomposite	185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	3	21	theme	Cd	649:650	arg1	cleanup					638:644	cleanup	638:644	cleanup of Cd(II) and Pb(II)	638:665	A series of adsorption experiments for cleanup of Cd(II) and Pb(II) were conducted to investigate the effects of lignin and sodium alginate on the graphene structure.					
29574641	4	22	theme	significant	801:811	arg1	increase					813:820	a significant increase	799:820	a significant increase in adsorption capacity contrast to porous graphene (PG)	799:876	It was found that PG/L/SA showed a significant increase in adsorption capacity contrast to porous graphene (PG).					
29574641	1	23	theme	porous	146:151	arg1	nanocomposite					185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite	109:197	nanocomposite	185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	0	24	theme	High	0:3	arg1	performance					5:15	High performance	0:15	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).	0:107	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).					
29574641	5	25	theme	as-prepared	883:893	arg1	material					895:902	The as-prepared material	879:902	The as-prepared material	879:902	The as-prepared material achieved the adsorption capacity for Cd(II) and Pb(II) of 79.88 and 226.24 mg/g, respectively.					
29574641	1	26	theme	graphene/lignin/sodium	153:174	arg1	nanocomposite					185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite	109:197	nanocomposite	185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	0	27	theme	3D	20:21	arg1	composite					62:70	3D porous graphene/lignin/sodium alginate composite	20:70	3D porous graphene/lignin/sodium alginate composite	20:70	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).					
29574641	1	28	dep	oxide	327:331	arg1	GO					334:335	GO	334:335	GO	334:335	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	6	29	theme	Langmuir	1055:1062	arg1	model					1073:1077	the Langmuir isotherm model	1051:1077	the Langmuir isotherm model	1051:1077	Meanwhile, the adsorption process matched well with the Langmuir isotherm model and the pseudo-second-order kinetic model.					
29574641	1	30	theme	alginate	176:183	arg1	nanocomposite					185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite	109:197	nanocomposite	185:197	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	6	31	theme	isotherm	1064:1071	arg1	model					1073:1077	the Langmuir isotherm model	1051:1077	the Langmuir isotherm model	1051:1077	Meanwhile, the adsorption process matched well with the Langmuir isotherm model and the pseudo-second-order kinetic model.					
29574641	1	32	theme	lignin	272:277	arg1	polymerization					254:267	hydrothermal polymerization	241:267	hydrothermal polymerization of lignin and sodium alginate	241:297	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	0	33	theme	Pb	101:102	arg1	adsorption					76:85	adsorption	76:85	adsorption of Cd(II) and Pb(II)	76:106	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).					
29574641	5	34	theme	226.24 mg/g	972:982	arg1	capacity					928:935	the adsorption capacity	913:935	the adsorption capacity for Cd(II) and Pb(II) of 79.88 and 226.24 mg/g	913:982	The as-prepared material achieved the adsorption capacity for Cd(II) and Pb(II) of 79.88 and 226.24 mg/g, respectively.					
29574641	2	35	theme	electron	434:441	arg1	microscopy					443:452	scanning electron microscopy	425:452	scanning electron microscopy	425:452	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	35	theme	electron	434:441	arg1	spectra					387:393	infrared spectra	378:393	infrared spectra	378:393	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	36	theme	infrared	378:385	arg1	analysis					415:422	thermo-gravimetric analysis	396:422	thermo-gravimetric analysis	396:422	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	36	theme	infrared	378:385	arg1	spectroscopy					479:490	X-ray photoelectron spectroscopy	459:490	X-ray photoelectron spectroscopy	459:490	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	36	theme	infrared	378:385	arg1	microscopy					443:452	scanning electron microscopy	425:452	scanning electron microscopy	425:452	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	36	theme	infrared	378:385	arg1	spectra					387:393	infrared spectra	378:393	infrared spectra	378:393	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	0	37	theme	graphene/lignin/sodium	30:51	arg1	composite					62:70	3D porous graphene/lignin/sodium alginate composite	20:70	3D porous graphene/lignin/sodium alginate composite	20:70	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).					
29574641	1	38	theme	aqueous	344:350	arg1	system					352:357	an aqueous system	341:357	an aqueous system	341:357	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	7	39	theme	metal	1238:1242	arg1	wastewater					1253:1262	metal smelting wastewater	1238:1262	metal smelting wastewater	1238:1262	Studies were also conducted to demonstrate the applicability of the sorbent to the removal of heavy metal ions from metal smelting wastewater.					
29574641	0	40	theme	porous	23:28	arg1	composite					62:70	3D porous graphene/lignin/sodium alginate composite	20:70	3D porous graphene/lignin/sodium alginate composite	20:70	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).					
29574641	1	41	theme	sodium	283:288	arg1	alginate					290:297	sodium alginate	283:297	sodium alginate	283:297	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	1	42	from	oxide	327:331	arg1	system					352:357	an aqueous system	341:357	an aqueous system	341:357	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	2	43	theme	thermo-gravimetric	396:413	arg1	analysis					415:422	thermo-gravimetric analysis	396:422	thermo-gravimetric analysis	396:422	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	43	theme	thermo-gravimetric	396:413	arg1	spectra					387:393	infrared spectra	378:393	infrared spectra	378:393	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	7	44	theme	heavy	1216:1220	arg1	ions					1228:1231	heavy metal ions	1216:1231	heavy metal ions	1216:1231	Studies were also conducted to demonstrate the applicability of the sorbent to the removal of heavy metal ions from metal smelting wastewater.					
29574641	0	45	theme	composite	62:70	arg1	performance					5:15	High performance	0:15	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).	0:107	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).					
29574641	1	46	theme	alginate	290:297	arg1	polymerization					254:267	hydrothermal polymerization	241:267	hydrothermal polymerization of lignin and sodium alginate	241:297	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	2	47	theme	functional	565:574	arg1	nanocomposite					584:596	this novel functional PG/L/SA nanocomposite	554:596	this novel functional PG/L/SA nanocomposite	554:596	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	48	theme	photoelectron	465:477	arg1	spectroscopy					479:490	X-ray photoelectron spectroscopy	459:490	X-ray photoelectron spectroscopy	459:490	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	48	theme	photoelectron	465:477	arg1	spectra					387:393	infrared spectra	378:393	infrared spectra	378:393	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	2	49	dep	morphology	526:535	arg1	the					522:524	the	522:524	the	522:524	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	0	50	theme	alginate	53:60	arg1	composite					62:70	3D porous graphene/lignin/sodium alginate composite	20:70	3D porous graphene/lignin/sodium alginate composite	20:70	High performance of 3D porous graphene/lignin/sodium alginate composite for adsorption of Cd(II) and Pb(II).					
29574641	6	51	theme	pseudo-second-order	1087:1105	arg1	model					1115:1119	the pseudo-second-order kinetic model	1083:1119	the pseudo-second-order kinetic model	1083:1119	Meanwhile, the adsorption process matched well with the Langmuir isotherm model and the pseudo-second-order kinetic model.					
29574641	2	52	theme	novel	559:563	arg1	nanocomposite					584:596	this novel functional PG/L/SA nanocomposite	554:596	this novel functional PG/L/SA nanocomposite	554:596	Fourier transform infrared spectra, thermo-gravimetric analysis, scanning electron microscopy, and X-ray photoelectron spectroscopy were employed to characterize the morphology and structure of this novel functional PG/L/SA nanocomposite.					
29574641	3	53	theme	lignin	712:717	arg1	effects					701:707	the effects	697:707	the effects of lignin and sodium alginate on the graphene structure	697:763	A series of adsorption experiments for cleanup of Cd(II) and Pb(II) were conducted to investigate the effects of lignin and sodium alginate on the graphene structure.					
29574641	3	54	theme	sodium	723:728	arg1	alginate					730:737	sodium alginate	723:737	sodium alginate	723:737	A series of adsorption experiments for cleanup of Cd(II) and Pb(II) were conducted to investigate the effects of lignin and sodium alginate on the graphene structure.					
29574641	3	55	theme	adsorption	611:620	arg1	experiments					622:632	adsorption experiments	611:632	adsorption experiments for cleanup of Cd(II) and Pb(II)	611:665	A series of adsorption experiments for cleanup of Cd(II) and Pb(II) were conducted to investigate the effects of lignin and sodium alginate on the graphene structure.					
29574641	7	56	theme	sorbent	1190:1196	arg1	applicability					1169:1181	the applicability	1165:1181	the applicability of the sorbent to the removal of heavy metal ions from metal smelting wastewater	1165:1262	Studies were also conducted to demonstrate the applicability of the sorbent to the removal of heavy metal ions from metal smelting wastewater.					
29574641	4	57	theme	porous	857:862	arg1	PG					874:875	PG	874:875	PG	874:875	It was found that PG/L/SA showed a significant increase in adsorption capacity contrast to porous graphene (PG).					
29574641	4	57	theme	porous	857:862	arg1	graphene					864:871	porous graphene	857:871	porous graphene (PG)	857:876	It was found that PG/L/SA showed a significant increase in adsorption capacity contrast to porous graphene (PG).					
29574641	5	58	theme	79.88	962:966	arg1	capacity					928:935	the adsorption capacity	913:935	the adsorption capacity for Cd(II) and Pb(II) of 79.88 and 226.24 mg/g	913:982	The as-prepared material achieved the adsorption capacity for Cd(II) and Pb(II) of 79.88 and 226.24 mg/g, respectively.					
29574641	3	59	theme	experiments	622:632	arg1	series					601:606	A series	599:606	A series of adsorption experiments for cleanup of Cd(II) and Pb(II)	599:665	A series of adsorption experiments for cleanup of Cd(II) and Pb(II) were conducted to investigate the effects of lignin and sodium alginate on the graphene structure.					
29574641	4	60	theme	adsorption	825:834	arg1	contrast					845:852	adsorption capacity contrast	825:852	adsorption capacity contrast to porous graphene (PG)	825:876	It was found that PG/L/SA showed a significant increase in adsorption capacity contrast to porous graphene (PG).					
29574641	1	61	theme	3D	211:212	arg1	PG/L/SA					214:220	3D PG/L/SA	211:220	3D PG/L/SA	211:220	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	4	62	from	increase	813:820	arg1	contrast					845:852	adsorption capacity contrast	825:852	adsorption capacity contrast to porous graphene (PG)	825:876	It was found that PG/L/SA showed a significant increase in adsorption capacity contrast to porous graphene (PG).					
29574641	7	63	theme	metal	1222:1226	arg1	ions					1228:1231	heavy metal ions	1216:1231	heavy metal ions	1216:1231	Studies were also conducted to demonstrate the applicability of the sorbent to the removal of heavy metal ions from metal smelting wastewater.					
29574641	6	64	theme	adsorption	1014:1023	arg1	Meanwhile					999:1007	Meanwhile	999:1007	Meanwhile	999:1007	Meanwhile, the adsorption process matched well with the Langmuir isotherm model and the pseudo-second-order kinetic model.					
29574641	6	64	theme	adsorption	1014:1023	arg1	process					1025:1031	the adsorption process	1010:1031	the adsorption process	1010:1031	Meanwhile, the adsorption process matched well with the Langmuir isotherm model and the pseudo-second-order kinetic model.					
29574641	1	65	from	presence	306:313	arg1	system					352:357	an aqueous system	341:357	an aqueous system	341:357	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	1	66	dep	nanocomposite	185:197	arg1	denoted					200:206	denoted	200:206	denoted as 3D PG/L/SA	200:220	A novel adsorbent, three-dimensional porous graphene/lignin/sodium alginate nanocomposite (denoted as 3D PG/L/SA) was fabricated by hydrothermal polymerization of lignin and sodium alginate in the presence of graphene oxide (GO) in an aqueous system.					
29574641	3	67	theme	graphene	746:753	arg1	structure					755:763	the graphene structure	742:763	the graphene structure	742:763	A series of adsorption experiments for cleanup of Cd(II) and Pb(II) were conducted to investigate the effects of lignin and sodium alginate on the graphene structure.					
31521379	7	0	theme	TAA-AAILs	1179:1187	arg1	length					1171:1176	alkyl chain length	1159:1176	alkyl chain length	1159:1176	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	0	theme	TAA-AAILs	1179:1187	arg1	concentration					1189:1201	TAA-AAILs concentration	1179:1201	TAA-AAILs concentration	1179:1201	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	6	1	theme	direct	918:923	arg1	it					901:902	it	901:902	it	901:902	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	6	1	theme	direct	918:923	arg1	evidence					940:947	the first direct and convincing evidence	908:947	the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems	908:1080	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	1	2	theme	tetraalkylammonium	155:172	arg1	liquids					191:197	tetraalkylammonium amino acid ionic liquids	155:197	tetraalkylammonium amino acid ionic liquids (TAA-AAILs)	155:209	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	2	theme	tetraalkylammonium	155:172	arg1	TAA-AAILs					200:208	TAA-AAILs	200:208	TAA-AAILs	200:208	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	6	3	dep	evidence	940:947	arg1	able					965:968	able	965:968	able	965:968	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	7	4	theme	applied	1252:1258	arg1	length					1171:1176	alkyl chain length	1159:1176	alkyl chain length	1159:1176	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	4	theme	applied	1252:1258	arg1	voltage					1260:1266	applied voltage	1252:1266	applied voltage	1252:1266	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	1	5	theme	native	348:353	arg1	β-CD					371:374	β-CD	371:374	β-CD	371:374	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	5	theme	native	348:353	arg1	selector					338:345	a conventional chiral selector	316:345	a conventional chiral selector	316:345	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	5	theme	native	348:353	arg1	β-cyclodextrin					355:368	native β-cyclodextrin	348:368	native β-cyclodextrin (β-CD)	348:375	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	6	theme	amino	174:178	arg1	liquids					191:197	tetraalkylammonium amino acid ionic liquids	155:197	tetraalkylammonium amino acid ionic liquids (TAA-AAILs)	155:209	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	6	theme	amino	174:178	arg1	TAA-AAILs					200:208	TAA-AAILs	200:208	TAA-AAILs	200:208	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	6	7	theme	enantiorecognition	992:1009	arg1	process					1011:1017	the enantiorecognition process	988:1017	the enantiorecognition process	988:1017	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	0	8	theme	capillary	92:100	arg1	electrophoresis					102:116	non-aqueous capillary electrophoresis	80:116	non-aqueous capillary electrophoresis for enantioseparation	80:138	Investigation of the synergistic effect of chiral ionic liquids as additives in non-aqueous capillary electrophoresis for enantioseparation.					
31521379	4	9	theme	reported	678:685	arg1	studies					717:723	previously reported chiral ILs-related aqueous CE studies	667:723	previously reported chiral ILs-related aqueous CE studies	667:723	Several interesting results were observed compared with previously reported chiral ILs-related aqueous CE studies.					
31521379	0	10	theme	non-aqueous	80:90	arg1	electrophoresis					102:116	non-aqueous capillary electrophoresis	80:116	non-aqueous capillary electrophoresis for enantioseparation	80:138	Investigation of the synergistic effect of chiral ionic liquids as additives in non-aqueous capillary electrophoresis for enantioseparation.					
31521379	10	11	theme	real	1629:1632	arg1	sample					1637:1642	a real AA sample	1627:1642	a real AA sample	1627:1642	The method was then successfully employed for the determination of enantiomeric impurities of a real AA sample.					
31521379	3	12	theme	molecular	506:514	arg1	study					524:528	a molecular docking study	504:528	a molecular docking study	504:528	A series of comparison experiments and a molecular docking study were performed to validate the synergistic effect of TAA-AAILs and β-CD in NACE.					
31521379	10	13	theme	impurities	1613:1622	arg1	determination					1583:1595	the determination	1579:1595	the determination of enantiomeric impurities of a real AA sample	1579:1642	The method was then successfully employed for the determination of enantiomeric impurities of a real AA sample.					
31521379	6	14	theme	chiral	1039:1044	arg1	systems					1074:1080	the conventional chiral selectors-based synergistic systems	1022:1080	the conventional chiral selectors-based synergistic systems	1022:1080	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	1	15	theme	acid	180:183	arg1	liquids					191:197	tetraalkylammonium amino acid ionic liquids	155:197	tetraalkylammonium amino acid ionic liquids (TAA-AAILs)	155:209	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	15	theme	acid	180:183	arg1	TAA-AAILs					200:208	TAA-AAILs	200:208	TAA-AAILs	200:208	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	5	16	theme	TAA-AAILs	774:782	arg1	enantioselectivity					752:769	the direct enantioselectivity	741:769	the direct enantioselectivity of TAA-AAILs	741:782	In particular, the direct enantioselectivity of TAA-AAILs was observed for the first time by using it as sole chiral selector in NACE.					
31521379	7	17	theme	length	1171:1176	arg1	terms					1150:1154	terms	1150:1154	terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc	1150:1271	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	18	theme	alkyl	1159:1163	arg1	etc					1269:1271	etc	1269:1271	etc	1269:1271	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	18	theme	alkyl	1159:1163	arg1	composition					1236:1246	electrolyte composition	1224:1246	electrolyte composition	1224:1246	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	18	theme	alkyl	1159:1163	arg1	concentration					1189:1201	TAA-AAILs concentration	1179:1201	TAA-AAILs concentration	1179:1201	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	18	theme	alkyl	1159:1163	arg1	voltage					1260:1266	applied voltage	1252:1266	applied voltage	1252:1266	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	18	theme	alkyl	1159:1163	arg1	length					1171:1176	alkyl chain length	1159:1176	alkyl chain length	1159:1176	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	18	theme	alkyl	1159:1163	arg1	concentration					1209:1221	β-CD concentration	1204:1221	β-CD concentration	1204:1221	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	19	theme	electrolyte	1224:1234	arg1	length					1171:1176	alkyl chain length	1159:1176	alkyl chain length	1159:1176	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	19	theme	electrolyte	1224:1234	arg1	composition					1236:1246	electrolyte composition	1224:1246	electrolyte composition	1224:1246	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	11	20	theme	ILs	1685:1687	arg1	use					1671:1673	the use	1667:1673	the use of chiral ILs as additives in NACE	1667:1708	This work proved that the use of chiral ILs as additives in NACE is a promising approach for enantioseparation.					
31521379	11	20	theme	ILs	1685:1687	arg1	approach					1725:1732	a promising approach	1713:1732	a promising approach for enantioseparation	1713:1754	This work proved that the use of chiral ILs as additives in NACE is a promising approach for enantioseparation.					
31521379	1	21	theme	ionic	185:189	arg1	liquids					191:197	tetraalkylammonium amino acid ionic liquids	155:197	tetraalkylammonium amino acid ionic liquids (TAA-AAILs)	155:209	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	21	theme	ionic	185:189	arg1	TAA-AAILs					200:208	TAA-AAILs	200:208	TAA-AAILs	200:208	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	7	22	theme	NACE	1113:1116	arg1	system					1118:1123	The new TAA-AAILs synergistic NACE system	1083:1123	The new TAA-AAILs synergistic NACE system	1083:1123	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	1	23	with	systems	303:309	arg1	selector					338:345	a conventional chiral selector	316:345	a conventional chiral selector	316:345	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	23	with	systems	303:309	arg1	β-cyclodextrin					355:368	native β-cyclodextrin	348:368	native β-cyclodextrin (β-CD)	348:375	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	8	24	theme	NMF	1410:1412	arg1	buffer					1414:1419	an NMF buffer	1407:1419	an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection	1407:1492	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	10	25	theme	enantiomeric	1600:1611	arg1	impurities					1613:1622	enantiomeric impurities	1600:1622	enantiomeric impurities of a real AA sample	1600:1642	The method was then successfully employed for the determination of enantiomeric impurities of a real AA sample.					
31521379	4	26	theme	ILs-related	694:704	arg1	studies					717:723	previously reported chiral ILs-related aqueous CE studies	667:723	previously reported chiral ILs-related aqueous CE studies	667:723	Several interesting results were observed compared with previously reported chiral ILs-related aqueous CE studies.					
31521379	6	27	theme	first	912:916	arg1	it					901:902	it	901:902	it	901:902	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	6	27	theme	first	912:916	arg1	evidence					940:947	the first direct and convincing evidence	908:947	the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems	908:1080	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	11	28	from	additives	1692:1700	arg1	NACE					1705:1708	NACE	1705:1708	NACE	1705:1708	This work proved that the use of chiral ILs as additives in NACE is a promising approach for enantioseparation.					
31521379	6	29	theme	convincing	929:938	arg1	it					901:902	it	901:902	it	901:902	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	6	29	theme	convincing	929:938	arg1	evidence					940:947	the first direct and convincing evidence	908:947	the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems	908:1080	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	3	30	theme	TAA-AAILs	583:591	arg1	effect					573:578	the synergistic effect	557:578	the synergistic effect of TAA-AAILs and β-CD in NACE	557:608	A series of comparison experiments and a molecular docking study were performed to validate the synergistic effect of TAA-AAILs and β-CD in NACE.					
31521379	2	31	theme	Excellent	378:386	arg1	enantioseparations					388:405	Excellent enantioseparations	378:405	Excellent enantioseparations of some dansyl-amino acid (Dns-AA) samples	378:448	Excellent enantioseparations of some dansyl-amino acid (Dns-AA) samples were achieved.					
31521379	0	32	theme	synergistic	21:31	arg1	effect					33:38	the synergistic effect	17:38	the synergistic effect of chiral ionic liquids	17:62	Investigation of the synergistic effect of chiral ionic liquids as additives in non-aqueous capillary electrophoresis for enantioseparation.					
31521379	7	33	theme	TAA-AAILs	1091:1099	arg1	system					1118:1123	The new TAA-AAILs synergistic NACE system	1083:1123	The new TAA-AAILs synergistic NACE system	1083:1123	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	3	34	theme	synergistic	561:571	arg1	effect					573:578	the synergistic effect	557:578	the synergistic effect of TAA-AAILs and β-CD in NACE	557:608	A series of comparison experiments and a molecular docking study were performed to validate the synergistic effect of TAA-AAILs and β-CD in NACE.					
31521379	8	35	theme	Best	1274:1277	arg1	enantioseparations					1279:1296	Best enantioseparations	1274:1296	Best enantioseparations of Dns-AAs	1274:1307	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	8	36	theme	Dns-AAs	1301:1307	arg1	enantioseparations					1279:1296	Best enantioseparations	1274:1296	Best enantioseparations of Dns-AAs	1274:1307	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	5	37	theme	chiral	836:841	arg1	selector					843:850	sole chiral selector	831:850	sole chiral selector in NACE	831:858	In particular, the direct enantioselectivity of TAA-AAILs was observed for the first time by using it as sole chiral selector in NACE.					
31521379	1	38	theme	synergistic	291:301	arg1	systems					303:309	synergistic systems	291:309	synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD)	291:375	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	7	39	theme	synergistic	1101:1111	arg1	system					1118:1123	The new TAA-AAILs synergistic NACE system	1083:1123	The new TAA-AAILs synergistic NACE system	1083:1123	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	6	40	theme	encouraging	873:883	arg1	finding					885:891	an encouraging finding	870:891	an encouraging finding	870:891	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	6	40	theme	encouraging	873:883	arg1	This					861:864	This	861:864	This	861:864	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	3	41	theme	docking	516:522	arg1	study					524:528	a molecular docking study	504:528	a molecular docking study	504:528	A series of comparison experiments and a molecular docking study were performed to validate the synergistic effect of TAA-AAILs and β-CD in NACE.					
31521379	5	42	theme	sole	831:834	arg1	selector					843:850	sole chiral selector	831:850	sole chiral selector in NACE	831:858	In particular, the direct enantioselectivity of TAA-AAILs was observed for the first time by using it as sole chiral selector in NACE.					
31521379	5	43	theme	first	805:809	arg1	time					811:814	the first time	801:814	the first time	801:814	In particular, the direct enantioselectivity of TAA-AAILs was observed for the first time by using it as sole chiral selector in NACE.					
31521379	6	44	theme	synergistic	1062:1072	arg1	systems					1074:1080	the conventional chiral selectors-based synergistic systems	1022:1080	the conventional chiral selectors-based synergistic systems	1022:1080	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	4	45	theme	chiral	687:692	arg1	studies					717:723	previously reported chiral ILs-related aqueous CE studies	667:723	previously reported chiral ILs-related aqueous CE studies	667:723	Several interesting results were observed compared with previously reported chiral ILs-related aqueous CE studies.					
31521379	2	46	theme	acid	428:431	arg1	samples					442:448	some dansyl-amino acid (Dns-AA) samples	410:448	some dansyl-amino acid (Dns-AA) samples	410:448	Excellent enantioseparations of some dansyl-amino acid (Dns-AA) samples were achieved.					
31521379	0	47	theme	effect	33:38	arg1	Investigation					0:12	Investigation	0:12	Investigation of the synergistic effect of chiral ionic liquids	0:62	Investigation of the synergistic effect of chiral ionic liquids as additives in non-aqueous capillary electrophoresis for enantioseparation.					
31521379	7	48	theme	new	1087:1089	arg1	system					1118:1123	The new TAA-AAILs synergistic NACE system	1083:1123	The new TAA-AAILs synergistic NACE system	1083:1123	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	8	49	theme	10 mM	1344:1348	arg1	tetramethylammonium-l-arginine					1350:1379	10 mM tetramethylammonium-l-arginine	1344:1379	10 mM tetramethylammonium-l-arginine (TMA-l-Arg)	1344:1391	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	8	49	theme	10 mM	1344:1348	arg1	TMA-l-Arg					1382:1390	TMA-l-Arg	1382:1390	TMA-l-Arg	1382:1390	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	2	50	theme	samples	442:448	arg1	enantioseparations					388:405	Excellent enantioseparations	378:405	Excellent enantioseparations of some dansyl-amino acid (Dns-AA) samples	378:448	Excellent enantioseparations of some dansyl-amino acid (Dns-AA) samples were achieved.					
31521379	5	51	located	observed	788:795	arg1	particular					729:738	particular	729:738	particular	729:738	In particular, the direct enantioselectivity of TAA-AAILs was observed for the first time by using it as sole chiral selector in NACE.					
31521379	5	51	located	observed	788:795	arg2	enantioselectivity					752:769	the direct enantioselectivity	741:769	the direct enantioselectivity of TAA-AAILs	741:782	In particular, the direct enantioselectivity of TAA-AAILs was observed for the first time by using it as sole chiral selector in NACE.					
31521379	2	52	theme	dansyl-amino	415:426	arg1	acid					428:431	dansyl-amino acid	415:431	some dansyl-amino acid (Dns-AA) samples	410:448	Excellent enantioseparations of some dansyl-amino acid (Dns-AA) samples were achieved.					
31521379	2	52	theme	dansyl-amino	415:426	arg1	Dns-AA					434:439	Dns-AA	434:439	Dns-AA	434:439	Excellent enantioseparations of some dansyl-amino acid (Dns-AA) samples were achieved.					
31521379	0	53	theme	ionic	50:54	arg1	liquids					56:62	chiral ionic liquids	43:62	chiral ionic liquids	43:62	Investigation of the synergistic effect of chiral ionic liquids as additives in non-aqueous capillary electrophoresis for enantioseparation.					
31521379	5	54	theme	direct	745:750	arg1	enantioselectivity					752:769	the direct enantioselectivity	741:769	the direct enantioselectivity of TAA-AAILs	741:782	In particular, the direct enantioselectivity of TAA-AAILs was observed for the first time by using it as sole chiral selector in NACE.					
31521379	8	55	contain	containing	1421:1430	arg2	pH					1466:1467	apparent pH 7.85	1457:1472	apparent pH 7.85	1457:1472	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	8	55	contain	containing	1421:1430	arg1	buffer					1414:1419	an NMF buffer	1407:1419	an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection	1407:1492	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	8	55	contain	containing	1421:1430	arg2	CA					1453:1454	35 mM CA	1447:1454	35 mM CA (apparent pH 7.85)	1447:1473	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	8	55	contain	containing	1421:1430	arg2	Tris					1438:1441	50 mM Tris	1432:1441	50 mM Tris	1432:1441	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	8	56	theme	35 mM	1447:1451	arg1	CA					1453:1454	35 mM CA	1447:1454	35 mM CA (apparent pH 7.85)	1447:1473	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	8	56	theme	35 mM	1447:1451	arg1	pH					1466:1467	apparent pH 7.85	1457:1472	apparent pH 7.85	1457:1472	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	11	57	theme	promising	1715:1723	arg1	use					1671:1673	the use	1667:1673	the use of chiral ILs as additives in NACE	1667:1708	This work proved that the use of chiral ILs as additives in NACE is a promising approach for enantioseparation.					
31521379	11	57	theme	promising	1715:1723	arg1	approach					1725:1732	a promising approach	1713:1732	a promising approach for enantioseparation	1713:1754	This work proved that the use of chiral ILs as additives in NACE is a promising approach for enantioseparation.					
31521379	8	58	theme	100 mM	1328:1333	arg1	β-CD					1335:1338	100 mM β-CD	1328:1338	100 mM β-CD	1328:1338	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	0	59	theme	chiral	43:48	arg1	liquids					56:62	chiral ionic liquids	43:62	chiral ionic liquids	43:62	Investigation of the synergistic effect of chiral ionic liquids as additives in non-aqueous capillary electrophoresis for enantioseparation.					
31521379	1	60	theme	conventional	318:329	arg1	selector					338:345	a conventional chiral selector	316:345	a conventional chiral selector	316:345	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	60	theme	conventional	318:329	arg1	β-cyclodextrin					355:368	native β-cyclodextrin	348:368	native β-cyclodextrin (β-CD)	348:375	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	3	61	theme	β-CD	597:600	arg1	effect					573:578	the synergistic effect	557:578	the synergistic effect of TAA-AAILs and β-CD in NACE	557:608	A series of comparison experiments and a molecular docking study were performed to validate the synergistic effect of TAA-AAILs and β-CD in NACE.					
31521379	5	62	from	selector	843:850	arg1	NACE					855:858	NACE	855:858	NACE	855:858	In particular, the direct enantioselectivity of TAA-AAILs was observed for the first time by using it as sole chiral selector in NACE.					
31521379	8	63	theme	UV	1481:1482	arg1	detection					1484:1492	UV detection	1481:1492	UV detection	1481:1492	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	1	64	theme	chiral	331:336	arg1	selector					338:345	a conventional chiral selector	316:345	a conventional chiral selector	316:345	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	64	theme	chiral	331:336	arg1	β-cyclodextrin					355:368	native β-cyclodextrin	348:368	native β-cyclodextrin (β-CD)	348:375	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	10	65	theme	sample	1637:1642	arg1	impurities					1613:1622	enantiomeric impurities	1600:1622	enantiomeric impurities of a real AA sample	1600:1642	The method was then successfully employed for the determination of enantiomeric impurities of a real AA sample.					
31521379	7	66	theme	chain	1165:1169	arg1	etc					1269:1271	etc	1269:1271	etc	1269:1271	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	66	theme	chain	1165:1169	arg1	composition					1236:1246	electrolyte composition	1224:1246	electrolyte composition	1224:1246	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	66	theme	chain	1165:1169	arg1	concentration					1189:1201	TAA-AAILs concentration	1179:1201	TAA-AAILs concentration	1179:1201	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	66	theme	chain	1165:1169	arg1	voltage					1260:1266	applied voltage	1252:1266	applied voltage	1252:1266	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	66	theme	chain	1165:1169	arg1	length					1171:1176	alkyl chain length	1159:1176	alkyl chain length	1159:1176	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	66	theme	chain	1165:1169	arg1	concentration					1209:1221	β-CD concentration	1204:1221	β-CD concentration	1204:1221	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	8	67	theme	50 mM	1432:1436	arg1	Tris					1438:1441	50 mM Tris	1432:1441	50 mM Tris	1432:1441	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	3	68	theme	comparison	477:486	arg1	experiments					488:498	comparison experiments	477:498	comparison experiments	477:498	A series of comparison experiments and a molecular docking study were performed to validate the synergistic effect of TAA-AAILs and β-CD in NACE.					
31521379	3	69	from	effect	573:578	arg1	NACE					605:608	NACE	605:608	NACE	605:608	A series of comparison experiments and a molecular docking study were performed to validate the synergistic effect of TAA-AAILs and β-CD in NACE.					
31521379	4	70	theme	Several	611:617	arg1	results					631:637	Several interesting results	611:637	Several interesting results	611:637	Several interesting results were observed compared with previously reported chiral ILs-related aqueous CE studies.					
31521379	8	71	theme	apparent	1457:1464	arg1	CA					1453:1454	35 mM CA	1447:1454	35 mM CA (apparent pH 7.85)	1447:1473	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	8	71	theme	apparent	1457:1464	arg1	pH					1466:1467	apparent pH 7.85	1457:1472	apparent pH 7.85	1457:1472	Best enantioseparations of Dns-AAs were obtained when 100 mM β-CD and 10 mM tetramethylammonium-l-arginine (TMA-l-Arg) were added in an NMF buffer containing 50 mM Tris and 35 mM CA (apparent pH 7.85) under UV detection.					
31521379	10	72	theme	AA	1634:1635	arg1	sample					1637:1642	a real AA sample	1627:1642	a real AA sample	1627:1642	The method was then successfully employed for the determination of enantiomeric impurities of a real AA sample.					
31521379	3	73	theme	experiments	488:498	arg1	study					524:528	a molecular docking study	504:528	a molecular docking study	504:528	A series of comparison experiments and a molecular docking study were performed to validate the synergistic effect of TAA-AAILs and β-CD in NACE.					
31521379	3	73	theme	experiments	488:498	arg1	series					467:472	A series	465:472	A series of comparison experiments	465:498	A series of comparison experiments and a molecular docking study were performed to validate the synergistic effect of TAA-AAILs and β-CD in NACE.					
31521379	6	74	theme	conventional	1026:1037	arg1	systems					1074:1080	the conventional chiral selectors-based synergistic systems	1022:1080	the conventional chiral selectors-based synergistic systems	1022:1080	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	9	75	theme	applied	1499:1505	arg1	voltage					1507:1513	The applied voltage	1495:1513	The applied voltage	1495:1513	The applied voltage was set at 30 kV.					
31521379	6	76	theme	selectors-based	1046:1060	arg1	systems					1074:1080	the conventional chiral selectors-based synergistic systems	1022:1080	the conventional chiral selectors-based synergistic systems	1022:1080	This was an encouraging finding because it was the first direct and convincing evidence that AAILs were able to participate in the enantiorecognition process in the conventional chiral selectors-based synergistic systems.					
31521379	11	77	theme	chiral	1678:1683	arg1	ILs					1685:1687	chiral ILs	1678:1687	chiral ILs	1678:1687	This work proved that the use of chiral ILs as additives in NACE is a promising approach for enantioseparation.					
31521379	4	78	theme	CE	714:715	arg1	studies					717:723	previously reported chiral ILs-related aqueous CE studies	667:723	previously reported chiral ILs-related aqueous CE studies	667:723	Several interesting results were observed compared with previously reported chiral ILs-related aqueous CE studies.					
31521379	1	79	theme	non-aqueous	233:243	arg1	NACE					272:275	NACE	272:275	NACE	272:275	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	79	theme	non-aqueous	233:243	arg1	electrophoresis					255:269	non-aqueous capillary electrophoresis	233:269	non-aqueous capillary electrophoresis (NACE)	233:276	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	0	80	theme	liquids	56:62	arg1	effect					33:38	the synergistic effect	17:38	the synergistic effect of chiral ionic liquids	17:62	Investigation of the synergistic effect of chiral ionic liquids as additives in non-aqueous capillary electrophoresis for enantioseparation.					
31521379	4	81	theme	interesting	619:629	arg1	results					631:637	Several interesting results	611:637	Several interesting results	611:637	Several interesting results were observed compared with previously reported chiral ILs-related aqueous CE studies.					
31521379	4	82	theme	aqueous	706:712	arg1	studies					717:723	previously reported chiral ILs-related aqueous CE studies	667:723	previously reported chiral ILs-related aqueous CE studies	667:723	Several interesting results were observed compared with previously reported chiral ILs-related aqueous CE studies.					
31521379	7	83	theme	β-CD	1204:1207	arg1	length					1171:1176	alkyl chain length	1159:1176	alkyl chain length	1159:1176	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	7	83	theme	β-CD	1204:1207	arg1	concentration					1209:1221	β-CD concentration	1204:1221	β-CD concentration	1204:1221	The new TAA-AAILs synergistic NACE system was further optimized in terms of alkyl chain length, TAA-AAILs concentration, β-CD concentration, electrolyte composition and applied voltage, etc.					
31521379	1	84	theme	capillary	245:253	arg1	NACE					272:275	NACE	272:275	NACE	272:275	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
31521379	1	84	theme	capillary	245:253	arg1	electrophoresis					255:269	non-aqueous capillary electrophoresis	233:269	non-aqueous capillary electrophoresis (NACE)	233:276	In this work, tetraalkylammonium amino acid ionic liquids (TAA-AAILs) were first applied to non-aqueous capillary electrophoresis (NACE) to establish synergistic systems with a conventional chiral selector, native β-cyclodextrin (β-CD).					
29649136	10	0	from	role	1423:1426	arg1	pools					1490:1494	the spinal motor pools	1473:1494	the spinal motor pools	1473:1494	Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.					
29649136	9	1	dep	are	1292:1294	arg1	2					1283:1283	2	1283:1283	2	1283:1283	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
29649136	5	2	from	motoneurones	912:923	arg1	heterogeneity					876:888	the molecular heterogeneity	862:888	the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns)	862:929	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	10	3	theme	motor	1484:1488	arg1	pools					1490:1494	the spinal motor pools	1473:1494	the spinal motor pools	1473:1494	Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.					
29649136	4	4	theme	functional	612:621	arg1	recovery					629:636	this functional motor recovery	607:636	this functional motor recovery	607:636	We aim to investigate the populations of PNN neurones involved in this functional motor recovery.					
29649136	0	5	theme	Alpha	83:87	arg1	Motoneurones					89:100	Alpha Motoneurones	83:100	Alpha Motoneurones	83:100	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.					
29649136	0	6	from	Nets	13:16	arg1	Motoneurones					28:39	Spinal Motoneurones	21:39	Spinal Motoneurones	21:39	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.					
29649136	7	7	theme	Mns	1083:1085	arg1	Mns					1083:1085	Mns	1083:1085	Mns	1083:1085	Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15-30% of Mns showed CSPG-positive but WFA-negative PNNs.					
29649136	7	7	theme	Mns	1083:1085	arg1	%					1078:1078	~15-30%	1072:1078	~15-30% of Mns	1072:1085	Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15-30% of Mns showed CSPG-positive but WFA-negative PNNs.					
29649136	1	8	theme	neuronal	176:183	arg1	sub-populations					185:199	neuronal sub-populations	176:199	neuronal sub-populations	176:199	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	5	9	theme	sulphate	780:787	arg1	CSPGs					804:808	CSPGs	804:808	CSPGs	804:808	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	9	theme	sulphate	780:787	arg1	proteoglycans					789:801	Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans	727:801	Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs)	727:809	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	9	theme	sulphate	780:787	arg1	aggrecan					822:829	aggrecan	822:829	aggrecan	822:829	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	7	10	theme	CSPG-positive	1094:1106	arg1	PNNs					1125:1128	CSPG-positive but WFA-negative PNNs	1094:1128	CSPG-positive but WFA-negative PNNs	1094:1128	Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15-30% of Mns showed CSPG-positive but WFA-negative PNNs.					
29649136	7	11	theme	WFA-negative	1112:1123	arg1	PNNs					1125:1128	CSPG-positive but WFA-negative PNNs	1094:1128	CSPG-positive but WFA-negative PNNs	1094:1128	Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15-30% of Mns showed CSPG-positive but WFA-negative PNNs.					
29649136	10	12	theme	PNNs	1431:1434	arg1	role					1423:1426	the role	1419:1426	the role of PNNs and their molecular heterogeneity in the spinal motor pools	1419:1494	Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.					
29649136	3	13	theme	various	415:421	arg1	regions					429:435	various brain regions	415:435	various brain regions	415:435	While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown.					
29649136	2	14	theme	functional	333:342	arg1	recovery					344:351	functional recovery	333:351	functional recovery	333:351	Enzymatically removing PNNs successfully enhances plasticity and thus functional recovery, particularly in spinal cord injury models.					
29649136	1	15	theme	extracellular	132:144	arg1	structures					153:162	extracellular matrix structures	132:162	extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity	132:260	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	1	15	theme	extracellular	132:144	arg1	nets					116:119	Perineuronal nets	103:119	Perineuronal nets (PNNs)	103:126	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	5	16	from	heterogeneity	876:888	arg1	motoneurones					912:923	rat spinal motoneurones	901:923	rat spinal motoneurones (Mns)	901:929	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	16	from	heterogeneity	876:888	arg1	Mns					926:928	Mns	926:928	Mns	926:928	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	17	theme	PNNs	893:896	arg1	heterogeneity					876:888	the molecular heterogeneity	862:888	the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns)	862:929	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	18	theme	choline	664:670	arg1	acetyltransferase					672:688	choline acetyltransferase	664:688	choline acetyltransferase (labelling motoneurones)	664:713	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	18	theme	choline	664:670	arg1	motoneurones					701:712	labelling motoneurones	691:712	labelling motoneurones	691:712	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	18	theme	choline	664:670	arg1	PNNs					716:719	PNNs	716:719	PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan	716:829	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	10	19	theme	spinal	1477:1482	arg1	pools					1490:1494	the spinal motor pools	1473:1494	the spinal motor pools	1473:1494	Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.					
29649136	5	20	theme	spinal	905:910	arg1	motoneurones					912:923	rat spinal motoneurones	901:923	rat spinal motoneurones (Mns)	901:929	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	20	theme	spinal	905:910	arg1	Mns					926:928	Mns	926:928	Mns	926:928	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	4	21	theme	motor	623:627	arg1	recovery					629:636	this functional motor recovery	607:636	this functional motor recovery	607:636	We aim to investigate the populations of PNN neurones involved in this functional motor recovery.					
29649136	9	22	from	differences	1296:1306	arg1	populations					1357:1367	their associated neuronal populations	1331:1367	their associated neuronal populations	1331:1367	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
29649136	9	22	from	differences	1296:1306	arg1	composition					1315:1325	PNN composition	1311:1325	PNN composition	1311:1325	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
29649136	10	23	theme	molecular	1446:1454	arg1	heterogeneity					1456:1468	their molecular heterogeneity	1440:1468	their molecular heterogeneity	1440:1468	Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.					
29649136	8	24	theme	alpha	1192:1196	arg1	Mns					1198:1200	alpha Mns	1192:1200	alpha Mns	1192:1200	Selective labelling revealed that aggrecan encircled ~90% of alpha Mns.					
29649136	3	25	theme	associated	483:492	arg1	populations					494:504	associated populations	483:504	associated populations	483:504	While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown.					
29649136	7	26	theme	spinal	1054:1059	arg1	Mns					1061:1063	the spinal Mns	1050:1063	the spinal Mns	1050:1063	Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15-30% of Mns showed CSPG-positive but WFA-negative PNNs.					
29649136	5	27	theme	labelling	691:699	arg1	acetyltransferase					672:688	choline acetyltransferase	664:688	choline acetyltransferase (labelling motoneurones)	664:713	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	27	theme	labelling	691:699	arg1	motoneurones					701:712	labelling motoneurones	691:712	labelling motoneurones	691:712	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	2	28	theme	spinal	370:375	arg1	injury					382:387	spinal cord injury	370:387	spinal cord injury models	370:394	Enzymatically removing PNNs successfully enhances plasticity and thus functional recovery, particularly in spinal cord injury models.					
29649136	6	29	theme	CSPG-positive	932:944	arg1	PNNs					946:949	CSPG-positive PNNs	932:949	CSPG-positive PNNs	932:949	CSPG-positive PNNs surrounded ~70-80% of Mns.					
29649136	0	30	theme	Perineuronal	0:11	arg1	Nets					13:16	Perineuronal Nets	0:16	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.	0:101	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.					
29649136	4	31	theme	neurones	586:593	arg1	populations					567:577	the populations	563:577	the populations of PNN neurones involved in this functional motor recovery	563:636	We aim to investigate the populations of PNN neurones involved in this functional motor recovery.					
29649136	1	32	theme	Perineuronal	103:114	arg1	structures					153:162	extracellular matrix structures	132:162	extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity	132:260	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	1	32	theme	Perineuronal	103:114	arg1	PNNs					122:125	PNNs	122:125	PNNs	122:125	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	1	32	theme	Perineuronal	103:114	arg1	nets					116:119	Perineuronal nets	103:119	Perineuronal nets (PNNs)	103:126	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	1	33	theme	central	216:222	arg1	system					232:237	the central nervous system	212:237	the central nervous system	212:237	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	3	34	theme	brain	423:427	arg1	regions					429:435	various brain regions	415:435	various brain regions	415:435	While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown.					
29649136	4	35	theme	PNN	582:584	arg1	neurones					586:593	PNN neurones	582:593	PNN neurones involved in this functional motor recovery	582:636	We aim to investigate the populations of PNN neurones involved in this functional motor recovery.					
29649136	10	36	theme	post-injury	1570:1580	arg1	recovery					1561:1568	functional recovery post-injury	1550:1580	functional recovery post-injury	1550:1580	Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.					
29649136	9	37	theme	neuronal	1348:1355	arg1	populations					1357:1367	their associated neuronal populations	1331:1367	their associated neuronal populations	1331:1367	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
29649136	1	38	theme	nervous	224:230	arg1	system					232:237	the central nervous system	212:237	the central nervous system	212:237	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	7	39	theme	PNNs	1020:1023	arg1	PNNs					1020:1023	the CSPG-positive PNNs	1002:1023	the CSPG-positive PNNs	1002:1023	Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15-30% of Mns showed CSPG-positive but WFA-negative PNNs.					
29649136	7	39	theme	PNNs	1020:1023	arg1	%					997:997	only ~60%	989:997	only ~60% of the CSPG-positive PNNs	989:1023	Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15-30% of Mns showed CSPG-positive but WFA-negative PNNs.					
29649136	9	40	theme	PNN	1311:1313	arg1	composition					1315:1325	PNN composition	1311:1325	PNN composition	1311:1325	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
29649136	5	41	theme	molecular	866:874	arg1	heterogeneity					876:888	the molecular heterogeneity	862:888	the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns)	862:929	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	3	42	theme	spinal	513:518	arg1	cord					520:523	the spinal cord	509:523	the spinal cord	509:523	While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown.					
29649136	10	43	theme	functional	1550:1559	arg1	recovery					1561:1568	functional recovery post-injury	1550:1580	functional recovery post-injury	1550:1580	Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.					
29649136	10	44	theme	heterogeneity	1456:1468	arg1	role					1423:1426	the role	1419:1426	the role of PNNs and their molecular heterogeneity in the spinal motor pools	1419:1494	Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.					
29649136	3	45	theme	composition	467:477	arg1	much					455:458	much	455:458	much	455:458	While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown.					
29649136	3	46	theme	populations	494:504	arg1	much					455:458	much	455:458	much	455:458	While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown.					
29649136	5	47	theme	chondroitin	768:778	arg1	sulphate					780:787	chondroitin sulphate	768:787	chondroitin sulphate	768:787	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	0	48	theme	Spinal	21:26	arg1	Motoneurones					28:39	Spinal Motoneurones	21:39	Spinal Motoneurones	21:39	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.					
29649136	3	49	from	populations	494:504	arg1	cord					520:523	the spinal cord	509:523	the spinal cord	509:523	While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown.					
29649136	5	50	theme	rat	901:903	arg1	motoneurones					912:923	rat spinal motoneurones	901:923	rat spinal motoneurones (Mns)	901:929	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	50	theme	rat	901:903	arg1	Mns					926:928	Mns	926:928	Mns	926:928	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	0	51	theme	Sulphate	54:61	arg1	Proteoglycan					63:74	Chondroitin Sulphate Proteoglycan	42:74	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.	0:101	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.					
29649136	8	52	theme	~90	1184:1186	arg1	%					1187:1187	~90%	1184:1187	~90% of alpha Mns	1184:1200	Selective labelling revealed that aggrecan encircled ~90% of alpha Mns.					
29649136	8	52	theme	~90	1184:1186	arg1	Mns					1198:1200	alpha Mns	1192:1200	alpha Mns	1192:1200	Selective labelling revealed that aggrecan encircled ~90% of alpha Mns.					
29649136	5	53	theme	Wisteria	727:734	arg1	agglutinin					747:756	Wisteria floribunda agglutinin	727:756	Wisteria floribunda agglutinin (WFA)	727:762	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	53	theme	Wisteria	727:734	arg1	WFA					759:761	WFA	759:761	WFA	759:761	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	54	gly	heterogeneity	876:888	arg1	PNNs					893:896	PNNs	893:896	PNNs in rat spinal motoneurones (Mns)	893:929	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	8	55	theme	Selective	1131:1139	arg1	labelling					1141:1149	Selective labelling	1131:1149	Selective labelling	1131:1149	Selective labelling revealed that aggrecan encircled ~90% of alpha Mns.					
29649136	0	56	theme	Chondroitin	42:52	arg1	Proteoglycan					63:74	Chondroitin Sulphate Proteoglycan	42:74	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.	0:101	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.					
29649136	9	57	theme	associated	1337:1346	arg1	populations					1357:1367	their associated neuronal populations	1331:1367	their associated neuronal populations	1331:1367	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
29649136	5	58	theme	floribunda	736:745	arg1	agglutinin					747:756	Wisteria floribunda agglutinin	727:756	Wisteria floribunda agglutinin (WFA)	727:762	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	58	theme	floribunda	736:745	arg1	WFA					759:761	WFA	759:761	WFA	759:761	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	9	59	theme	spinal	1249:1254	arg1	PNNs					1256:1259	spinal PNNs	1249:1259	spinal PNNs better than WFA	1249:1275	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
29649136	6	60	theme	Mns	973:975	arg1	Mns					973:975	Mns	973:975	Mns	973:975	CSPG-positive PNNs surrounded ~70-80% of Mns.					
29649136	6	60	theme	Mns	973:975	arg1	%					968:968	~70-80%	962:968	~70-80% of Mns	962:975	CSPG-positive PNNs surrounded ~70-80% of Mns.					
29649136	5	61	theme	agglutinin	747:756	arg1	CSPGs					804:808	CSPGs	804:808	CSPGs	804:808	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	61	theme	agglutinin	747:756	arg1	proteoglycans					789:801	Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans	727:801	Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs)	727:809	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	61	theme	agglutinin	747:756	arg1	aggrecan					822:829	aggrecan	822:829	aggrecan	822:829	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	3	62	dep	composition	467:477	arg1	the					463:465	the	463:465	the	463:465	While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown.					
29649136	2	63	theme	injury	382:387	arg1	models					389:394	spinal cord injury models	370:394	spinal cord injury models	370:394	Enzymatically removing PNNs successfully enhances plasticity and thus functional recovery, particularly in spinal cord injury models.					
29649136	5	64	from	PNNs	893:896	arg1	motoneurones					912:923	rat spinal motoneurones	901:923	rat spinal motoneurones (Mns)	901:929	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	5	64	from	PNNs	893:896	arg1	Mns					926:928	Mns	926:928	Mns	926:928	Immunohistochemistry for choline acetyltransferase (labelling motoneurones), PNNs using Wisteria floribunda agglutinin (WFA) and chondroitin sulphate proteoglycans (CSPGs), including aggrecan, was performed to characterise the molecular heterogeneity of PNNs in rat spinal motoneurones (Mns).					
29649136	9	65	theme	better	1261:1266	arg1	PNNs					1256:1259	spinal PNNs	1249:1259	spinal PNNs better than WFA	1249:1275	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
29649136	7	66	theme	CSPG-positive	1006:1018	arg1	PNNs					1020:1023	the CSPG-positive PNNs	1002:1023	the CSPG-positive PNNs	1002:1023	Using WFA, only ~60% of the CSPG-positive PNNs co-localised with WFA in the spinal Mns, while ~15-30% of Mns showed CSPG-positive but WFA-negative PNNs.					
29649136	2	67	theme	cord	377:380	arg1	injury					382:387	spinal cord injury	370:387	spinal cord injury models	370:394	Enzymatically removing PNNs successfully enhances plasticity and thus functional recovery, particularly in spinal cord injury models.					
29649136	9	68	dep	labels	1242:1247	arg1	1					1230:1230	1	1230:1230	1	1230:1230	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
29649136	10	69	theme	targeted	1519:1526	arg1	strategies					1528:1537	targeted strategies	1519:1537	targeted strategies	1519:1537	Insights into the role of PNNs and their molecular heterogeneity in the spinal motor pools could aid in designing targeted strategies to enhance functional recovery post-injury.					
29649136	0	70	dep	Nets	13:16	arg1	Proteoglycan					63:74	Chondroitin Sulphate Proteoglycan	42:74	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.	0:101	Perineuronal Nets in Spinal Motoneurones: Chondroitin Sulphate Proteoglycan around Alpha Motoneurones.					
29649136	3	71	from	composition	467:477	arg1	cord					520:523	the spinal cord	509:523	the spinal cord	509:523	While PNNs within various brain regions are well studied, much of the composition and associated populations in the spinal cord is yet unknown.					
29649136	1	72	theme	matrix	146:151	arg1	structures					153:162	extracellular matrix structures	132:162	extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity	132:260	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	1	72	theme	matrix	146:151	arg1	nets					116:119	Perineuronal nets	103:119	Perineuronal nets (PNNs)	103:126	Perineuronal nets (PNNs) are extracellular matrix structures surrounding neuronal sub-populations throughout the central nervous system, regulating plasticity.					
29649136	8	73	theme	Mns	1198:1200	arg1	%					1187:1187	~90%	1184:1187	~90% of alpha Mns	1184:1200	Selective labelling revealed that aggrecan encircled ~90% of alpha Mns.					
29649136	8	73	theme	Mns	1198:1200	arg1	Mns					1198:1200	alpha Mns	1192:1200	alpha Mns	1192:1200	Selective labelling revealed that aggrecan encircled ~90% of alpha Mns.					
29649136	9	74	theme	spinal	1381:1386	arg1	cord					1388:1391	the spinal cord	1377:1391	the spinal cord	1377:1391	The results indicate that (1) aggrecan labels spinal PNNs better than WFA, and (2) there are differences in PNN composition and their associated neuronal populations between the spinal cord and cortex.					
30054498	8	0	theme	regression	1199:1208	arg1	analysis					1210:1217	Partial least squares regression analysis	1177:1217	Partial least squares regression analysis of FTIR-MS data	1177:1233	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	7	1	from	p < 0.05	1138:1145	arg1	regression					1164:1173	multivariable regression	1150:1173	multivariable regression	1150:1173	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	7	2	theme	tensile	1023:1029	arg1	modulus					1031:1037	the tensile modulus	1019:1037	the tensile modulus (p < 0.05 in multivariable regression)	1019:1076	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	2	3	theme	maturation	381:390	arg1	levels					392:397	four different maturation levels	366:397	four different maturation levels (newborn, 5-month-old, 11-month-old and adult)	366:444	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	7	4	theme	collagen	925:932	arg1	predictors					1005:1014	important predictors	995:1014	important predictors of the tensile modulus (p < 0.05 in multivariable regression)	995:1076	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	7	4	theme	collagen	925:932	arg1	content					934:940	The collagen content	921:940	The collagen content	921:940	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	1	5	theme	cartilage	263:271	arg1	function					273:280	articular cartilage function	253:280	articular cartilage function	253:280	Articular cartilage undergoes structural and biochemical changes during maturation, but the knowledge on how these changes relate to articular cartilage function at different stages of maturation is lacking.					
30054498	0	6	theme	cartilage	80:88	arg1	structure					13:21	structure	13:21	structure	13:21	Composition, structure and tensile biomechanical properties of equine articular cartilage during growth and maturation.					
30054498	0	6	theme	cartilage	80:88	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition, structure and tensile biomechanical properties of equine articular cartilage during growth and maturation.					
30054498	0	6	theme	cartilage	80:88	arg1	properties					49:58	tensile biomechanical properties	27:58	tensile biomechanical properties	27:58	Composition, structure and tensile biomechanical properties of equine articular cartilage during growth and maturation.					
30054498	9	7	with	changes	1419:1425	arg1	depth					1432:1436	depth	1432:1436	depth	1432:1436	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	2	8	theme	different	371:379	arg1	levels					392:397	four different maturation levels	366:397	four different maturation levels (newborn, 5-month-old, 11-month-old and adult)	366:444	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	6	9	theme	arcade-like	896:906	arg1	orientation					908:918	an arcade-like orientation	893:918	an arcade-like orientation	893:918	The collagen orientation developed with age into an arcade-like orientation.					
30054498	9	10	theme	months	1562:1567	arg1	age					1550:1552	the age	1546:1552	the age of 5-11 months	1546:1567	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	7	11	theme	multivariable	1150:1162	arg1	regression					1164:1173	multivariable regression	1150:1173	multivariable regression	1150:1173	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	3	12	theme	polarized	561:569	arg1	microscopy					577:586	polarized light microscopy	561:586	polarized light microscopy	561:586	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	3	13	theme	light	571:575	arg1	microscopy					577:586	polarized light microscopy	561:586	polarized light microscopy	561:586	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	2	14	theme	cartilage	345:353	arg1	samples					355:361	Equine articular cartilage samples	328:361	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult)	328:444	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	8	15	theme	data	1230:1233	arg1	analysis					1210:1217	Partial least squares regression analysis	1177:1217	Partial least squares regression analysis of FTIR-MS data	1177:1233	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	4	16	theme	breaking	736:743	arg1	energy					745:750	the breaking energy lowest	732:757	the breaking energy lowest	732:757	The tensile modulus was highest and the breaking energy lowest in the newborn group.					
30054498	2	17	dep	collected	451:459	arg1	N = 25					462:467	N = 25	462:467	N = 25	462:467	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	7	18	theme	important	995:1003	arg1	predictors					1005:1014	important predictors	995:1014	important predictors of the tensile modulus (p < 0.05 in multivariable regression)	995:1076	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	7	18	theme	important	995:1003	arg1	content					934:940	The collagen content	921:940	The collagen content	921:940	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	3	19	dep	Fourier	502:508	arg1	transform					510:518	transform	510:518	transform infrared microspectroscopy (FTIR-MS)	510:555	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	9	20	theme	typical	1499:1505	arg1	properties					1507:1516	the typical properties	1495:1516	the typical properties of mature tissue reached at the age of 5-11 months	1495:1567	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	1	21	theme	different	285:293	arg1	stages					295:300	different stages	285:300	different stages of maturation	285:314	Articular cartilage undergoes structural and biochemical changes during maturation, but the knowledge on how these changes relate to articular cartilage function at different stages of maturation is lacking.					
30054498	7	22	dep	energy	1130:1135	arg1	p < 0.05					1138:1145	p < 0.05	1138:1145	p < 0.05 in multivariable regression	1138:1173	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	7	23	theme	collagen	969:976	arg1	orientation					978:988	collagen orientation	969:988	collagen orientation	969:988	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	2	24	dep	levels	392:397	arg1	5-month-old					409:419	5-month-old	409:419	5-month-old	409:419	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	2	24	dep	levels	392:397	arg1	newborn					400:406	newborn	400:406	newborn	400:406	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	2	24	dep	levels	392:397	arg1	adult					439:443	adult	439:443	adult	439:443	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	2	24	dep	levels	392:397	arg1	11-month-old					422:433	11-month-old	422:433	11-month-old	422:433	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	8	25	theme	accurate	1244:1251	arg1	predictions					1253:1263	accurate predictions	1244:1263	accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65)	1244:1333	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	8	26	theme	squares	1191:1197	arg1	analysis					1210:1217	Partial least squares regression analysis	1177:1217	Partial least squares regression analysis of FTIR-MS data	1177:1233	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	8	27	theme	least	1185:1189	arg1	squares					1191:1197	Partial least squares	1177:1197	Partial least squares regression analysis of FTIR-MS data	1177:1233	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	2	28	theme	articular	335:343	arg1	samples					355:361	Equine articular cartilage samples	328:361	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult)	328:444	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	4	29	theme	tensile	700:706	arg1	highest					720:726	highest	720:726	highest	720:726	The tensile modulus was highest and the breaking energy lowest in the newborn group.					
30054498	4	29	theme	tensile	700:706	arg1	modulus					708:714	The tensile modulus	696:714	The tensile modulus	696:714	The tensile modulus was highest and the breaking energy lowest in the newborn group.					
30054498	2	30	theme	Equine	328:333	arg1	samples					355:361	Equine articular cartilage samples	328:361	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult)	328:444	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	8	31	theme	breaking	1308:1315	arg1	energy					1317:1322	the breaking energy	1304:1322	the breaking energy (r = 0.65)	1304:1333	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	8	31	theme	breaking	1308:1315	arg1	r = 0.65					1325:1332	r = 0.65	1325:1332	r = 0.65	1325:1332	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	8	32	theme	FTIR-MS	1222:1228	arg1	data					1230:1233	FTIR-MS data	1222:1233	FTIR-MS data	1222:1233	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	8	33	theme	tensile	1273:1279	arg1	r = 0.79					1290:1297	r = 0.79	1290:1297	r = 0.79	1290:1297	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	8	33	theme	tensile	1273:1279	arg1	modulus					1281:1287	the tensile modulus	1269:1287	the tensile modulus (r = 0.79)	1269:1298	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	3	34	theme	Biomechanical	471:483	arg1	testing					493:499	Biomechanical tensile testing	471:499	Biomechanical tensile testing	471:499	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	1	35	theme	maturation	305:314	arg1	stages					295:300	different stages	285:300	different stages of maturation	285:314	Articular cartilage undergoes structural and biochemical changes during maturation, but the knowledge on how these changes relate to articular cartilage function at different stages of maturation is lacking.					
30054498	7	36	theme	breaking	1121:1128	arg1	energy					1130:1135	the breaking energy	1117:1135	the breaking energy (p < 0.05 in multivariable regression)	1117:1174	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	3	37	theme	cartilage	671:679	arg1	properties					647:656	the tensile, biochemical and structural properties	607:656	the tensile, biochemical and structural properties of articular cartilage, respectively	607:693	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	4	38	theme	newborn	766:772	arg1	group					774:778	the newborn group	762:778	the newborn group	762:778	The tensile modulus was highest and the breaking energy lowest in the newborn group.					
30054498	7	39	dep	modulus	1031:1037	arg1	p < 0.05					1040:1047	p < 0.05	1040:1047	p < 0.05	1040:1047	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	3	40	theme	tensile	485:491	arg1	testing					493:499	Biomechanical tensile testing	471:499	Biomechanical tensile testing	471:499	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	0	41	theme	biomechanical	35:47	arg1	properties					49:58	tensile biomechanical properties	27:58	tensile biomechanical properties	27:58	Composition, structure and tensile biomechanical properties of equine articular cartilage during growth and maturation.					
30054498	9	42	theme	articular	1389:1397	arg1	cartilage					1399:1407	equine articular cartilage	1382:1407	equine articular cartilage	1382:1407	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	3	43	theme	tensile	611:617	arg1	properties					647:656	the tensile, biochemical and structural properties	607:656	the tensile, biochemical and structural properties of articular cartilage, respectively	607:693	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	9	44	theme	tissue	1528:1533	arg1	properties					1507:1516	the typical properties	1495:1516	the typical properties of mature tissue reached at the age of 5-11 months	1495:1567	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	0	45	theme	tensile	27:33	arg1	properties					49:58	tensile biomechanical properties	27:58	tensile biomechanical properties	27:58	Composition, structure and tensile biomechanical properties of equine articular cartilage during growth and maturation.					
30054498	9	46	theme	cartilage	1399:1407	arg1	structure					1369:1377	structure	1369:1377	structure	1369:1377	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	9	46	theme	cartilage	1399:1407	arg1	composition					1353:1363	composition	1353:1363	composition	1353:1363	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	5	47	theme	proteoglycan	802:813	arg1	contents					815:822	the proteoglycan contents	798:822	the proteoglycan contents	798:822	The collagen and the proteoglycan contents increased with age.					
30054498	7	48	theme	multivariable	1052:1064	arg1	regression					1066:1075	multivariable regression	1052:1075	multivariable regression	1052:1075	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	3	49	used	used	593:596	arg2	testing					493:499	Biomechanical tensile testing	471:499	Biomechanical tensile testing	471:499	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	3	49	used	used	593:596	arg2	microscopy					577:586	polarized light microscopy	561:586	polarized light microscopy	561:586	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	3	49	used	used	593:596	arg2	Fourier					502:508	Fourier	502:508	Fourier transform infrared microspectroscopy (FTIR-MS)	502:555	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	9	50	dep	composition	1353:1363	arg1	the					1349:1351	the	1349:1351	the	1349:1351	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	3	51	theme	articular	661:669	arg1	cartilage					671:679	articular cartilage	661:679	articular cartilage	661:679	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	1	52	theme	Articular	120:128	arg1	cartilage					130:138	Articular cartilage	120:138	Articular cartilage	120:138	Articular cartilage undergoes structural and biochemical changes during maturation, but the knowledge on how these changes relate to articular cartilage function at different stages of maturation is lacking.					
30054498	1	53	theme	biochemical	165:175	arg1	changes					177:183	structural and biochemical changes	150:183	structural and biochemical changes	150:183	Articular cartilage undergoes structural and biochemical changes during maturation, but the knowledge on how these changes relate to articular cartilage function at different stages of maturation is lacking.					
30054498	9	54	theme	mature	1521:1526	arg1	tissue					1528:1533	mature tissue	1521:1533	mature tissue	1521:1533	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	7	55	theme	proteoglycan	943:954	arg1	content					956:962	proteoglycan content	943:962	proteoglycan content	943:962	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	3	56	theme	structural	636:645	arg1	properties					647:656	the tensile, biochemical and structural properties	607:656	the tensile, biochemical and structural properties of articular cartilage, respectively	607:693	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	9	57	theme	equine	1382:1387	arg1	cartilage					1399:1407	equine articular cartilage	1382:1407	equine articular cartilage	1382:1407	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	4	58	theme	lowest	752:757	arg1	energy					745:750	the breaking energy lowest	732:757	the breaking energy lowest	732:757	The tensile modulus was highest and the breaking energy lowest in the newborn group.					
30054498	3	59	dep	transform	510:518	arg1	infrared					520:527	infrared	520:527	transform infrared microspectroscopy (FTIR-MS)	510:555	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	7	60	theme	modulus	1031:1037	arg1	predictors					1005:1014	important predictors	995:1014	important predictors of the tensile modulus (p < 0.05 in multivariable regression)	995:1076	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	7	60	theme	modulus	1031:1037	arg1	content					934:940	The collagen content	921:940	The collagen content	921:940	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	1	61	theme	structural	150:159	arg1	changes					177:183	structural and biochemical changes	150:183	structural and biochemical changes	150:183	Articular cartilage undergoes structural and biochemical changes during maturation, but the knowledge on how these changes relate to articular cartilage function at different stages of maturation is lacking.					
30054498	8	62	theme	Partial	1177:1183	arg1	squares					1191:1197	Partial least squares	1177:1197	Partial least squares regression analysis of FTIR-MS data	1177:1233	Partial least squares regression analysis of FTIR-MS data provided accurate predictions for the tensile modulus (r = 0.79) and the breaking energy (r = 0.65).					
30054498	0	63	theme	articular	70:78	arg1	cartilage					80:88	equine articular cartilage	63:88	equine articular cartilage	63:88	Composition, structure and tensile biomechanical properties of equine articular cartilage during growth and maturation.					
30054498	6	64	theme	collagen	848:855	arg1	orientation					857:867	The collagen orientation	844:867	The collagen orientation	844:867	The collagen orientation developed with age into an arcade-like orientation.					
30054498	3	65	theme	biochemical	620:630	arg1	properties					647:656	the tensile, biochemical and structural properties	607:656	the tensile, biochemical and structural properties of articular cartilage, respectively	607:693	Biomechanical tensile testing, Fourier transform infrared microspectroscopy (FTIR-MS) and polarized light microscopy were used to study the tensile, biochemical and structural properties of articular cartilage, respectively.					
30054498	7	66	from	p < 0.05	1040:1047	arg1	regression					1066:1075	multivariable regression	1052:1075	multivariable regression	1052:1075	The collagen content, proteoglycan content, and collagen orientation were important predictors of the tensile modulus (p < 0.05 in multivariable regression) and correlated significantly also with the breaking energy (p < 0.05 in multivariable regression).					
30054498	0	67	theme	equine	63:68	arg1	cartilage					80:88	equine articular cartilage	63:88	equine articular cartilage	63:88	Composition, structure and tensile biomechanical properties of equine articular cartilage during growth and maturation.					
30054498	9	68	theme	functional	1449:1458	arg1	properties					1460:1469	functional properties	1449:1469	functional properties	1449:1469	To conclude, the composition and structure of equine articular cartilage undergoes changes with depth that alter functional properties during maturation, with the typical properties of mature tissue reached at the age of 5-11 months.					
30054498	2	69	theme	levels	392:397	arg1	samples					355:361	Equine articular cartilage samples	328:361	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult)	328:444	Equine articular cartilage samples of four different maturation levels (newborn, 5-month-old, 11-month-old and adult) were collected (N = 25).					
30054498	1	70	theme	articular	253:261	arg1	cartilage					263:271	articular cartilage	253:271	articular cartilage function	253:280	Articular cartilage undergoes structural and biochemical changes during maturation, but the knowledge on how these changes relate to articular cartilage function at different stages of maturation is lacking.					
30350976	5	0	theme	P	758:758	arg1	<					760:760	P < 0.05	758:765	P < 0.05	758:765	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	6	1	theme	protein	971:977	arg1	content					960:966	content	960:966	content of protein	960:977	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	6	1	theme	protein	971:977	arg1	content					937:943	the content	933:943	the content of dry matter	933:957	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	6	1	theme	protein	971:977	arg1	quality					992:998	protein quality	984:998	protein quality	984:998	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	7	2	theme	selection	1080:1088	arg1	processes					1090:1098	selection processes	1080:1098	selection processes	1080:1098	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	6	3	theme	dry	948:950	arg1	matter					952:957	dry matter	948:957	dry matter	948:957	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	4	4	theme	reducing	583:590	arg1	sugars					592:597	reducing sugars	583:597	reducing sugars	583:597	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	7	5	with	processes	1090:1098	arg1	characteristics					1138:1152	advantageous characteristics	1125:1152	advantageous characteristics in molecular breeding	1125:1174	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	3	6	theme	two-dimensional	518:532	arg1	electrophoresis					534:548	two-dimensional electrophoresis	518:548	two-dimensional electrophoresis	518:548	Patatin-based proteomic distances were assessed between 15 table and/or processing potato cultivars from profiles of patatin obtained by two-dimensional electrophoresis.					
30350976	6	7	theme	matter	952:957	arg1	content					960:966	content	960:966	content of protein	960:977	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	6	7	theme	matter	952:957	arg1	content					937:943	the content	933:943	the content of dry matter	933:957	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	6	7	theme	matter	952:957	arg1	quality					992:998	protein quality	984:998	protein quality	984:998	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	5	8	theme	proteomic	717:725	arg1	distances					727:735	proteomic distances	717:735	proteomic distances	717:735	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	6	9	theme	Proteomic	858:866	arg1	distances					868:876	Proteomic distances	858:876	Proteomic distances	858:876	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	2	10	theme	association	258:268	arg1	study					270:274	a first association study	250:274	a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits	250:378	Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits.					
30350976	1	11	theme	tuber	122:126	arg1	protein					136:142	the major tuber storage protein	112:142	the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties	112:231	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	1	11	theme	tuber	122:126	arg1	Patatin					101:107	Patatin	101:107	Patatin	101:107	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	7	12	from	characteristics	1138:1152	arg1	breeding					1167:1174	molecular breeding	1157:1174	molecular breeding	1157:1174	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	5	13	theme	ash	814:816	arg1	content					803:809	the content	799:809	the content of ash, dry matter, and essential amino acids	799:855	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	2	14	theme	first	252:256	arg1	study					270:274	a first association study	250:274	a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits	250:378	Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits.					
30350976	1	15	theme	multiple	159:166	arg1	isoforms					168:175	multiple isoforms	159:175	multiple isoforms highly variable across potato ( S. tuberosum) varieties	159:231	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	4	16	theme	sugars	592:597	arg1	content					555:561	The content	551:561	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition	551:648	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	5	17	from	differences	784:794	arg1	content					803:809	the content	799:809	the content of ash, dry matter, and essential amino acids	799:855	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	0	18	dep	Quality	85:91	arg1	Traits					93:98	Traits	93:98	Traits	93:98	Association of Patatin-Based Proteomic Distances with Potato ( Solanum tuberosum L.) Quality Traits.					
30350976	4	19	theme	composition	638:648	arg1	content					555:561	The content	551:561	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition	551:648	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	7	20	dep	screening	1066:1074	arg1	the					1062:1064	the	1062:1064	the	1062:1064	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	4	21	theme	dry	571:573	arg1	matter					575:580	dry matter	571:580	dry matter	571:580	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	3	22	from	profiles	486:493	arg1	table					440:444	15 table and/or processing potato cultivars	437:479	table	440:444	Patatin-based proteomic distances were assessed between 15 table and/or processing potato cultivars from profiles of patatin obtained by two-dimensional electrophoresis.					
30350976	2	23	theme	quality	365:371	arg1	traits					373:378	tuber quality traits	359:378	tuber quality traits	359:378	Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits.					
30350976	7	24	theme	molecular	1157:1165	arg1	breeding					1167:1174	molecular breeding	1157:1174	molecular breeding	1157:1174	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	5	25	theme	matter	823:828	arg1	content					803:809	the content	799:809	the content of ash, dry matter, and essential amino acids	799:855	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	2	26	theme	variability	283:293	arg1	study					270:274	a first association study	250:274	a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits	250:378	Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits.					
30350976	2	27	theme	tuber	359:363	arg1	traits					373:378	tuber quality traits	359:378	tuber quality traits	359:378	Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits.					
30350976	0	28	theme	Proteomic	29:37	arg1	Distances					39:47	Patatin-Based Proteomic Distances	15:47	Patatin-Based Proteomic Distances	15:47	Association of Patatin-Based Proteomic Distances with Potato ( Solanum tuberosum L.) Quality Traits.					
30350976	4	29	theme	ash	566:568	arg1	content					555:561	The content	551:561	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition	551:648	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	7	30	theme	patatin-based	1016:1028	arg1	distances					1040:1048	patatin-based proteomic distances	1016:1048	patatin-based proteomic distances	1016:1048	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	2	31	from	differences	344:354	arg1	traits					373:378	tuber quality traits	359:378	tuber quality traits	359:378	Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits.					
30350976	3	32	theme	potato	464:469	arg1	cultivars					471:479	15 table and/or processing potato cultivars	437:479	cultivars	471:479	Patatin-based proteomic distances were assessed between 15 table and/or processing potato cultivars from profiles of patatin obtained by two-dimensional electrophoresis.					
30350976	0	33	theme	Patatin-Based	15:27	arg1	Distances					39:47	Patatin-Based Proteomic Distances	15:47	Patatin-Based Proteomic Distances	15:47	Association of Patatin-Based Proteomic Distances with Potato ( Solanum tuberosum L.) Quality Traits.					
30350976	4	34	theme	matter	575:580	arg1	content					555:561	The content	551:561	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition	551:648	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	7	35	theme	proteomic	1030:1038	arg1	distances					1040:1048	patatin-based proteomic distances	1016:1048	patatin-based proteomic distances	1016:1048	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	1	36	theme	potato	200:205	arg1	varieties					223:231	potato ( S. tuberosum) varieties	200:231	potato ( S. tuberosum) varieties	200:231	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	7	37	theme	cultivars	1110:1118	arg1	screening					1066:1074	screening	1066:1074	screening	1066:1074	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	7	37	theme	cultivars	1110:1118	arg1	processes					1090:1098	selection processes	1080:1098	selection processes	1080:1098	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	4	38	theme	cultivar	687:694	arg1	tubers					672:677	tubers	672:677	tubers of each cultivar	672:694	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	5	39	dep	significantly	742:754	arg1	<					760:760	P < 0.05	758:765	P < 0.05	758:765	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	1	40	theme	variable	184:191	arg1	isoforms					168:175	multiple isoforms	159:175	multiple isoforms highly variable across potato ( S. tuberosum) varieties	159:231	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	4	41	theme	acid	633:636	arg1	composition					638:648	amino acid composition	627:648	amino acid composition	627:648	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	0	42	theme	Distances	39:47	arg1	Association					0:10	Association	0:10	Association of Patatin-Based Proteomic Distances with Potato ( Solanum tuberosum L.)	0:83	Association of Patatin-Based Proteomic Distances with Potato ( Solanum tuberosum L.) Quality Traits.					
30350976	2	43	with	cultivars	323:331	arg1	differences					344:354	their differences	338:354	their differences in tuber quality traits	338:378	Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits.					
30350976	0	44	dep	Potato	54:59	arg1	L.					81:82	Solanum tuberosum L.	63:82	Solanum tuberosum L.	63:82	Association of Patatin-Based Proteomic Distances with Potato ( Solanum tuberosum L.) Quality Traits.					
30350976	5	45	theme	dry	819:821	arg1	matter					823:828	dry matter	819:828	dry matter	819:828	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	3	46	theme	processing	453:462	arg1	cultivars					471:479	15 table and/or processing potato cultivars	437:479	cultivars	471:479	Patatin-based proteomic distances were assessed between 15 table and/or processing potato cultivars from profiles of patatin obtained by two-dimensional electrophoresis.					
30350976	3	47	theme	Patatin-based	381:393	arg1	distances					405:413	Patatin-based proteomic distances	381:413	Patatin-based proteomic distances	381:413	Patatin-based proteomic distances were assessed between 15 table and/or processing potato cultivars from profiles of patatin obtained by two-dimensional electrophoresis.					
30350976	5	48	theme	amino	845:849	arg1	acids					851:855	essential amino acids	835:855	essential amino acids	835:855	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	6	49	theme	protein	984:990	arg1	quality					992:998	protein quality	984:998	protein quality	984:998	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	7	50	with	screening	1066:1074	arg1	characteristics					1138:1152	advantageous characteristics	1125:1152	advantageous characteristics in molecular breeding	1125:1174	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	3	51	theme	proteomic	395:403	arg1	distances					405:413	Patatin-based proteomic distances	381:413	Patatin-based proteomic distances	381:413	Patatin-based proteomic distances were assessed between 15 table and/or processing potato cultivars from profiles of patatin obtained by two-dimensional electrophoresis.					
30350976	3	52	theme	patatin	498:504	arg1	profiles					486:493	profiles	486:493	profiles of patatin obtained by two-dimensional electrophoresis	486:548	Patatin-based proteomic distances were assessed between 15 table and/or processing potato cultivars from profiles of patatin obtained by two-dimensional electrophoresis.					
30350976	7	53	theme	advantageous	1125:1136	arg1	characteristics					1138:1152	advantageous characteristics	1125:1152	advantageous characteristics in molecular breeding	1125:1174	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	7	54	theme	potato	1103:1108	arg1	cultivars					1110:1118	potato cultivars	1103:1118	potato cultivars	1103:1118	In conclusion, patatin-based proteomic distances can shorten the screening and selection processes of potato cultivars with advantageous characteristics in molecular breeding.					
30350976	0	55	with	Association	0:10	arg1	Potato					54:59	Potato	54:59	Potato ( Solanum tuberosum L.)	54:83	Association of Patatin-Based Proteomic Distances with Potato ( Solanum tuberosum L.) Quality Traits.					
30350976	1	56	theme	major	116:120	arg1	protein					136:142	the major tuber storage protein	112:142	the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties	112:231	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	1	56	theme	major	116:120	arg1	Patatin					101:107	Patatin	101:107	Patatin	101:107	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	5	57	theme	essential	835:843	arg1	acids					851:855	essential amino acids	835:855	essential amino acids	835:855	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	2	58	theme	patatin	298:304	arg1	isoforms					306:313	patatin isoforms	298:313	patatin isoforms	298:313	Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits.					
30350976	2	59	theme	isoforms	306:313	arg1	variability					283:293	the variability	279:293	the variability of patatin isoforms between cultivars with their differences in tuber quality traits	279:378	Here, we report a first association study of the variability of patatin isoforms between cultivars with their differences in tuber quality traits.					
30350976	4	60	theme	amino	627:631	arg1	composition					638:648	amino acid composition	627:648	amino acid composition	627:648	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	1	61	theme	storage	128:134	arg1	protein					136:142	the major tuber storage protein	112:142	the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties	112:231	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	1	61	theme	storage	128:134	arg1	Patatin					101:107	Patatin	101:107	Patatin	101:107	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	4	62	theme	protein	614:620	arg1	content					555:561	The content	551:561	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition	551:648	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	1	63	dep	potato	200:205	arg1	tuberosum					212:220	S. tuberosum	209:220	S. tuberosum	209:220	Patatin is the major tuber storage protein constituted by multiple isoforms highly variable across potato ( S. tuberosum) varieties.					
30350976	4	64	theme	starch	600:605	arg1	content					555:561	The content	551:561	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition	551:648	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
30350976	5	65	theme	acids	851:855	arg1	content					803:809	the content	799:809	the content of ash, dry matter, and essential amino acids	799:855	Results showed that proteomic distances were significantly ( P < 0.05) associated with differences in the content of ash, dry matter, and essential amino acids.					
30350976	6	66	theme	outlier	905:911	arg1	cultivars					913:921	outlier cultivars	905:921	outlier cultivars regarding the content of dry matter, content of protein, and protein quality	905:998	Proteomic distances were also able to identify outlier cultivars regarding the content of dry matter, content of protein, and protein quality.					
30350976	4	67	theme	total	608:612	arg1	protein					614:620	total protein	608:620	total protein	608:620	The content of ash, dry matter, reducing sugars, starch, total protein, and amino acid composition was also evaluated in tubers of each cultivar.					
31911355	4	0	theme	good	537:540	arg1	characteristics					550:564	good sensory characteristics	537:564	good sensory characteristics	537:564	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	3	1	theme	high	303:306	arg1	potential					308:316	high potential	303:316	high potential due to being a rich source of starch and many bioactive compounds	303:382	For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds.					
31911355	4	2	theme	Sweet	462:466	arg1	beer					475:478	a Sweet potato beer	460:478	a Sweet potato beer	460:478	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	7	3	located	found	867:871	arg2	it					860:861	it	860:861	it	860:861	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	7	3	located	found	867:871	arg1	conclusion					848:857	conclusion	848:857	conclusion	848:857	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	7	4	theme	nutraceutical	947:959	arg1	properties					961:970	nutraceutical properties	947:970	nutraceutical properties	947:970	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	5	5	theme	Beer	567:570	arg1	samples					572:578	Beer samples	567:578	Beer samples	567:578	Beer samples showed increased antioxidant activity especially due to β-carotene and their total phenolic content.					
31911355	3	6	theme	rich	333:336	arg1	source					338:343	a rich source	331:343	a rich source of starch and many bioactive compounds	331:382	For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds.					
31911355	4	7	theme	best	426:429	arg1	condition					439:447	the best process condition	422:447	the best process condition	422:447	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	7	8	theme	bioactive	1003:1011	arg1	compounds					1013:1021	bioactive compounds	1003:1021	bioactive compounds	1003:1021	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	1	9	dep	beverages	118:126	arg1	worldwide					128:136	worldwide	128:136	the most consumed alcoholic beverages worldwide	90:136	Beer has been one of the most consumed alcoholic beverages worldwide.					
31911355	4	10	theme	sensory	542:548	arg1	characteristics					550:564	good sensory characteristics	537:564	good sensory characteristics	537:564	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	0	11	theme	potato	56:61	arg1	beer					63:66	Beauregard sweet potato beer	39:66	Beauregard sweet potato beer	39:66	Physicochemical and sensory profile of Beauregard sweet potato beer.					
31911355	5	12	theme	increased	587:595	arg1	activity					609:616	increased antioxidant activity	587:616	increased antioxidant activity especially due to β-carotene	587:645	Beer samples showed increased antioxidant activity especially due to β-carotene and their total phenolic content.					
31911355	6	13	theme	sensory	784:790	arg1	characteristics					807:821	sensory and functional characteristics	784:821	sensory and functional characteristics of the finished beer	784:842	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	6	14	theme	phytochemical	685:697	arg1	profile					699:705	The phytochemical profile	681:705	The phytochemical profile of sweet potato biocompounds	681:734	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	3	15	theme	bioactive	364:372	arg1	compounds					374:382	many bioactive compounds	359:382	many bioactive compounds	359:382	For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds.					
31911355	0	16	theme	Beauregard	39:48	arg1	beer					63:66	Beauregard sweet potato beer	39:66	Beauregard sweet potato beer	39:66	Physicochemical and sensory profile of Beauregard sweet potato beer.					
31911355	7	17	theme	compounds	1013:1021	arg1	composition					988:998	its rich composition	979:998	its rich composition of bioactive compounds	979:1021	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	3	18	theme	starch	348:353	arg1	source					338:343	a rich source	331:343	a rich source of starch and many bioactive compounds	331:382	For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds.					
31911355	4	19	theme	process	431:437	arg1	condition					439:447	the best process condition	422:447	the best process condition	422:447	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	6	20	theme	adjunct	773:779	arg1	effect					758:763	a direct effect	749:763	a direct effect of this adjunct on sensory and functional characteristics of the finished beer	749:842	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	4	21	theme	nutritional	494:504	arg1	properties					522:531	enhanced nutritional and antioxidant properties	485:531	enhanced nutritional and antioxidant properties	485:531	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	7	22	theme	promising	907:915	arg1	adjunct					917:923	a promising adjunct	905:923	a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds	905:1021	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	7	22	theme	promising	907:915	arg1	potato					895:900	Beauregard sweet potato	878:900	Beauregard sweet potato	878:900	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	0	23	theme	sensory	20:26	arg1	profile					28:34	sensory profile	20:34	sensory profile	20:34	Physicochemical and sensory profile of Beauregard sweet potato beer.					
31911355	2	24	theme	adjuncts	169:176	arg1	incorporation					152:164	the incorporation	148:164	the incorporation of adjuncts in the beer	148:188	However, the incorporation of adjuncts in the beer can add new organoleptic and functional characteristics to the beverage.					
31911355	3	25	theme	compounds	374:382	arg1	source					338:343	a rich source	331:343	a rich source of starch and many bioactive compounds	331:382	For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds.					
31911355	4	26	theme	antioxidant	510:520	arg1	properties					522:531	enhanced nutritional and antioxidant properties	485:531	enhanced nutritional and antioxidant properties	485:531	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	4	27	theme	study	401:405	arg1	aim					389:391	The aim	385:391	The aim of this study	385:405	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	2	28	theme	organoleptic	202:213	arg1	characteristics					230:244	new organoleptic and functional characteristics	198:244	new organoleptic and functional characteristics	198:244	However, the incorporation of adjuncts in the beer can add new organoleptic and functional characteristics to the beverage.					
31911355	3	29	theme	many	359:362	arg1	compounds					374:382	many bioactive compounds	359:382	many bioactive compounds	359:382	For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds.					
31911355	4	30	theme	potato	468:473	arg1	beer					475:478	a Sweet potato beer	460:478	a Sweet potato beer	460:478	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	3	31	theme	sweet	284:288	arg1	potato					290:295	Beauregard sweet potato	273:295	Beauregard sweet potato	273:295	For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds.					
31911355	0	32	theme	beer	63:66	arg1	Physicochemical					0:14	Physicochemical	0:14	Physicochemical	0:14	Physicochemical and sensory profile of Beauregard sweet potato beer.					
31911355	0	32	theme	beer	63:66	arg1	profile					28:34	sensory profile	20:34	sensory profile	20:34	Physicochemical and sensory profile of Beauregard sweet potato beer.					
31911355	2	33	theme	new	198:200	arg1	characteristics					230:244	new organoleptic and functional characteristics	198:244	new organoleptic and functional characteristics	198:244	However, the incorporation of adjuncts in the beer can add new organoleptic and functional characteristics to the beverage.					
31911355	6	34	theme	direct	751:756	arg1	effect					758:763	a direct effect	749:763	a direct effect of this adjunct on sensory and functional characteristics of the finished beer	749:842	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	0	35	theme	sweet	50:54	arg1	beer					63:66	Beauregard sweet potato beer	39:66	Beauregard sweet potato beer	39:66	Physicochemical and sensory profile of Beauregard sweet potato beer.					
31911355	4	36	theme	enhanced	485:492	arg1	properties					522:531	enhanced nutritional and antioxidant properties	485:531	enhanced nutritional and antioxidant properties	485:531	The aim of this study was to develop the best process condition to produce a Sweet potato beer with enhanced nutritional and antioxidant properties and good sensory characteristics.					
31911355	5	37	theme	total	657:661	arg1	content					672:678	their total phenolic content	651:678	their total phenolic content	651:678	Beer samples showed increased antioxidant activity especially due to β-carotene and their total phenolic content.					
31911355	6	38	theme	beer	839:842	arg1	characteristics					807:821	sensory and functional characteristics	784:821	sensory and functional characteristics of the finished beer	784:842	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	5	39	theme	antioxidant	597:607	arg1	activity					609:616	increased antioxidant activity	587:616	increased antioxidant activity especially due to β-carotene	587:645	Beer samples showed increased antioxidant activity especially due to β-carotene and their total phenolic content.					
31911355	7	40	theme	rich	983:986	arg1	composition					988:998	its rich composition	979:998	its rich composition of bioactive compounds	979:1021	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	1	41	theme	consumed	99:106	arg1	beverages					118:126	the most consumed alcoholic beverages worldwide	90:136	the most consumed alcoholic beverages worldwide	90:136	Beer has been one of the most consumed alcoholic beverages worldwide.					
31911355	5	42	theme	phenolic	663:670	arg1	content					672:678	their total phenolic content	651:678	their total phenolic content	651:678	Beer samples showed increased antioxidant activity especially due to β-carotene and their total phenolic content.					
31911355	6	43	theme	finished	830:837	arg1	beer					839:842	the finished beer	826:842	the finished beer	826:842	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	6	44	theme	functional	796:805	arg1	characteristics					807:821	sensory and functional characteristics	784:821	sensory and functional characteristics of the finished beer	784:842	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	1	45	theme	alcoholic	108:116	arg1	beverages					118:126	the most consumed alcoholic beverages worldwide	90:136	the most consumed alcoholic beverages worldwide	90:136	Beer has been one of the most consumed alcoholic beverages worldwide.					
31911355	3	46	theme	Beauregard	273:282	arg1	potato					290:295	Beauregard sweet potato	273:295	Beauregard sweet potato	273:295	For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds.					
31911355	6	47	theme	biocompounds	723:734	arg1	profile					699:705	The phytochemical profile	681:705	The phytochemical profile of sweet potato biocompounds	681:734	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	1	48	theme	beverages	118:126	arg1	one					83:85	one	83:85	one	83:85	Beer has been one of the most consumed alcoholic beverages worldwide.					
31911355	1	48	theme	beverages	118:126	arg1	beverages					118:126	the most consumed alcoholic beverages worldwide	90:136	the most consumed alcoholic beverages worldwide	90:136	Beer has been one of the most consumed alcoholic beverages worldwide.					
31911355	7	49	theme	Beauregard	878:887	arg1	adjunct					917:923	a promising adjunct	905:923	a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds	905:1021	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	7	49	theme	Beauregard	878:887	arg1	potato					895:900	Beauregard sweet potato	878:900	Beauregard sweet potato	878:900	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	6	50	from	effect	758:763	arg1	characteristics					807:821	sensory and functional characteristics	784:821	sensory and functional characteristics of the finished beer	784:842	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	2	51	from	incorporation	152:164	arg1	beer					185:188	the beer	181:188	the beer	181:188	However, the incorporation of adjuncts in the beer can add new organoleptic and functional characteristics to the beverage.					
31911355	6	52	theme	potato	716:721	arg1	biocompounds					723:734	sweet potato biocompounds	710:734	sweet potato biocompounds	710:734	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
31911355	7	53	theme	sweet	889:893	arg1	adjunct					917:923	a promising adjunct	905:923	a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds	905:1021	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	7	53	theme	sweet	889:893	arg1	potato					895:900	Beauregard sweet potato	878:900	Beauregard sweet potato	878:900	In conclusion, it was found that Beauregard sweet potato is a promising adjunct for beer brewing with nutraceutical properties due to its rich composition of bioactive compounds.					
31911355	3	54	theme	due	318:320	arg1	potential					308:316	high potential	303:316	high potential due to being a rich source of starch and many bioactive compounds	303:382	For this, Beauregard sweet potato shows high potential due to being a rich source of starch and many bioactive compounds.					
31911355	2	55	theme	functional	219:228	arg1	characteristics					230:244	new organoleptic and functional characteristics	198:244	new organoleptic and functional characteristics	198:244	However, the incorporation of adjuncts in the beer can add new organoleptic and functional characteristics to the beverage.					
31911355	6	56	theme	sweet	710:714	arg1	biocompounds					723:734	sweet potato biocompounds	710:734	sweet potato biocompounds	710:734	The phytochemical profile of sweet potato biocompounds demonstrated a direct effect of this adjunct on sensory and functional characteristics of the finished beer.					
30859611	0	0	theme	equine	84:89	arg1	model					107:111	the equine carpal fracture model	80:111	the equine carpal fracture model	80:111	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	4	1	theme	molecular	695:703	arg1	distribution					712:723	the molecular weight distribution	691:723	the molecular weight distribution of HA	691:729	Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis.					
30859611	1	2	from	variations	166:175	arg1	fluid					189:193	synovial fluid composition and lubrication	180:221	fluid	189:193	The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture.					
30859611	1	2	from	variations	166:175	arg1	lubrication					211:221	lubrication	211:221	lubrication	211:221	The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture.					
30859611	4	3	theme	Lubricin	601:608	arg1	concentrations					635:648	Lubricin and hyaluronic acid (HA) concentrations	601:648	Lubricin and hyaluronic acid (HA) concentrations	601:648	Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis.					
30859611	0	4	from	changes	9:15	arg1	composition					35:45	synovial fluid composition	20:45	synovial fluid composition	20:45	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	0	4	from	changes	9:15	arg1	lubrication					65:75	elastoviscous lubrication	51:75	elastoviscous lubrication	51:75	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	0	4	from	changes	9:15	arg1	model					107:111	the equine carpal fracture model	80:111	the equine carpal fracture model	80:111	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	4	5	theme	weight	705:710	arg1	distribution					712:723	the molecular weight distribution	691:723	the molecular weight distribution of HA	691:729	Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis.					
30859611	2	6	from	fracture	331:338	arg1	joint					361:365	the middle carpal joint	343:365	the middle carpal joint of four horses	343:380	Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control.					
30859611	10	7	theme	synovial	1672:1679	arg1	fluid					1681:1685	synovial fluid	1672:1685	synovial fluid washout during arthroscopy	1672:1712	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	0	8	theme	fracture	98:105	arg1	model					107:111	the equine carpal fracture model	80:111	the equine carpal fracture model	80:111	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	10	9	theme	HA	1537:1538	arg1	distribution					1557:1568	HA molecular weight distribution	1537:1568	HA molecular weight distribution	1537:1568	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	3	10	theme	serial	551:556	arg1	timepoints					558:567	serial timepoints	551:567	serial timepoints	551:567	Horses were exercised on a high-speed treadmill, and synovial fluid was collected pre-operatively and at serial timepoints until 75 days post-operatively.					
30859611	4	11	theme	hyaluronic	614:623	arg1	HA					631:632	HA	631:632	HA	631:632	Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis.					
30859611	4	11	theme	hyaluronic	614:623	arg1	acid					625:628	hyaluronic acid	614:628	hyaluronic acid (HA)	614:633	Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis.					
30859611	0	12	theme	carpal	91:96	arg1	model					107:111	the equine carpal fracture model	80:111	the equine carpal fracture model	80:111	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	10	13	theme	weight	1550:1555	arg1	distribution					1557:1568	HA molecular weight distribution	1537:1568	HA molecular weight distribution	1537:1568	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	4	14	theme	HA	728:729	arg1	distribution					712:723	the molecular weight distribution	691:723	the molecular weight distribution of HA	691:729	Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis.					
30859611	3	15	theme	high-speed	473:482	arg1	treadmill					484:492	a high-speed treadmill	471:492	a high-speed treadmill	471:492	Horses were exercised on a high-speed treadmill, and synovial fluid was collected pre-operatively and at serial timepoints until 75 days post-operatively.					
30859611	1	16	theme	temporal	157:164	arg1	variations					166:175	temporal variations	157:175	temporal variations in synovial fluid composition and lubrication following articular fracture	157:250	The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture.					
30859611	10	17	theme	molecular	1540:1548	arg1	distribution					1557:1568	HA molecular weight distribution	1537:1568	HA molecular weight distribution	1537:1568	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	11	18	theme	Orthopaedic	1722:1732	arg1	Research					1734:1741	© 2019 Orthopaedic Research	1715:1741	© 2019 Orthopaedic Research Society.	1715:1750	© 2019 Orthopaedic Research Society.					
30859611	4	19	theme	acid	625:628	arg1	concentrations					635:648	Lubricin and hyaluronic acid (HA) concentrations	601:648	Lubricin and hyaluronic acid (HA) concentrations	601:648	Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis.					
30859611	2	20	theme	contralateral	392:404	arg1	limb					406:409	the contralateral limb	388:409	the contralateral limb served as a sham-operated control	388:443	Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control.					
30859611	10	21	theme	transition	1586:1595	arg1	number					1597:1602	transition number	1586:1602	transition number	1586:1602	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	9	22	theme	elastoviscous	1334:1346	arg1	mode					1348:1351	elastoviscous mode	1334:1351	elastoviscous mode	1334:1351	While boundary and elastoviscous mode friction coefficients did not vary, the transition number, representing the shift between these modes, was lower.					
30859611	4	23	theme	gel	748:750	arg1	electrophoresis					752:766	gel electrophoresis	748:766	gel electrophoresis	748:766	Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis.					
30859611	10	24	theme	similar	1509:1515	arg1	derangements					1517:1528	similar derangements	1509:1528	similar derangements in HA, HA molecular weight distribution, viscosity, and transition number	1509:1602	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	10	25	theme	sham-operated	1625:1637	arg1	limbs					1639:1643	the sham-operated limbs	1621:1643	the sham-operated limbs	1621:1643	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	0	26	theme	Temporal	0:7	arg1	changes					9:15	Temporal changes	0:15	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.	0:112	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	6	27	theme	HA	970:971	arg1	concentrations					973:986	HA concentrations	970:986	HA concentrations	970:986	HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75.					
30859611	2	28	theme	osteochondral	317:329	arg1	fracture					331:338	an osteochondral fracture	314:338	an osteochondral fracture in the middle carpal joint of four horses	314:380	Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control.					
30859611	6	29	from	day	1142:1144	arg1	termination					1127:1137	the study termination	1117:1137	the study termination at day 75	1117:1147	HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75.					
30859611	1	30	theme	synovial	180:187	arg1	fluid					189:193	synovial fluid composition and lubrication	180:221	fluid	189:193	The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture.					
30859611	0	31	theme	synovial	20:27	arg1	composition					35:45	synovial fluid composition	20:45	synovial fluid composition	20:45	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	10	32	theme	OA	1499:1500	arg1	limbs					1502:1506	the OA limbs	1495:1506	the OA limbs	1495:1506	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	5	33	theme	custom	937:942	arg1	tribometer					944:953	a custom tribometer	935:953	a custom tribometer	935:953	Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively.					
30859611	2	34	theme	sham-operated	423:435	arg1	control					437:443	a sham-operated control	421:443	a sham-operated control	421:443	Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control.					
30859611	5	35	theme	lubrication	850:860	arg1	modes					841:845	all modes	837:845	all modes of lubrication	837:860	Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively.					
30859611	9	36	theme	friction	1353:1360	arg1	coefficients					1362:1373	friction coefficients	1353:1373	friction coefficients	1353:1373	While boundary and elastoviscous mode friction coefficients did not vary, the transition number, representing the shift between these modes, was lower.					
30859611	1	37	theme	study	136:140	arg1	objective					118:126	The objective	114:126	The objective of this study	114:140	The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture.					
30859611	5	38	theme	cartilage	798:806	arg1	coefficients					817:828	Synovial fluid viscosity and cartilage friction coefficients	769:828	coefficients	817:828	Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively.					
30859611	3	39	theme	synovial	499:506	arg1	fluid					508:512	synovial fluid	499:512	synovial fluid	499:512	Horses were exercised on a high-speed treadmill, and synovial fluid was collected pre-operatively and at serial timepoints until 75 days post-operatively.					
30859611	6	40	theme	study	1121:1125	arg1	termination					1127:1137	the study termination	1117:1137	the study termination at day 75	1117:1147	HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75.					
30859611	10	41	located	observed	1609:1616	arg1	limbs					1639:1643	the sham-operated limbs	1621:1643	the sham-operated limbs	1621:1643	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	10	41	located	observed	1609:1616	arg2	derangements					1517:1528	similar derangements	1509:1528	similar derangements in HA, HA molecular weight distribution, viscosity, and transition number	1509:1602	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	0	42	theme	fluid	29:33	arg1	composition					35:45	synovial fluid composition	20:45	synovial fluid composition	20:45	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	9	43	dep	boundary	1321:1328	arg1	coefficients					1362:1373	friction coefficients	1353:1373	friction coefficients	1353:1373	While boundary and elastoviscous mode friction coefficients did not vary, the transition number, representing the shift between these modes, was lower.					
30859611	5	44	theme	friction	808:815	arg1	coefficients					817:828	Synovial fluid viscosity and cartilage friction coefficients	769:828	coefficients	817:828	Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively.					
30859611	7	45	theme	Lubricin	1150:1157	arg1	concentrations					1159:1172	Lubricin concentrations	1150:1172	Lubricin concentrations	1150:1172	Lubricin concentrations increased after surgery to a greater extent in the OA as compared to sham-operated limbs.					
30859611	6	46	theme	molecular	1044:1052	arg1	weight					1054:1059	high molecular weight HA	1039:1062	high molecular weight HA (>6.1MDa)	1039:1072	HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75.					
30859611	6	46	theme	molecular	1044:1052	arg1	>6.1MDa					1065:1071	>6.1MDa	1065:1071	>6.1MDa	1065:1071	HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75.					
30859611	0	47	theme	elastoviscous	51:63	arg1	lubrication					65:75	elastoviscous lubrication	51:75	elastoviscous lubrication	51:75	Temporal changes in synovial fluid composition and elastoviscous lubrication in the equine carpal fracture model.					
30859611	2	48	theme	carpal	354:359	arg1	joint					361:365	the middle carpal joint	343:365	the middle carpal joint of four horses	343:380	Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control.					
30859611	9	49	theme	transition	1393:1402	arg1	number					1404:1409	the transition number	1389:1409	the transition number	1389:1409	While boundary and elastoviscous mode friction coefficients did not vary, the transition number, representing the shift between these modes, was lower.					
30859611	9	49	theme	transition	1393:1402	arg1	lower					1460:1464	lower	1460:1464	lower	1460:1464	While boundary and elastoviscous mode friction coefficients did not vary, the transition number, representing the shift between these modes, was lower.					
30859611	7	50	from	extent	1211:1216	arg1	OA					1225:1226	the OA	1221:1226	the OA	1221:1226	Lubricin concentrations increased after surgery to a greater extent in the OA as compared to sham-operated limbs.					
30859611	10	51	from	limbs	1502:1506	arg1	pronounced					1481:1490	pronounced	1481:1490	pronounced	1481:1490	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	2	52	theme	middle	347:352	arg1	joint					361:365	the middle carpal joint	343:365	the middle carpal joint of four horses	343:380	Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control.					
30859611	11	53	theme	©	1715:1715	arg1	Research					1734:1741	© 2019 Orthopaedic Research	1715:1741	© 2019 Orthopaedic Research Society.	1715:1750	© 2019 Orthopaedic Research Society.					
30859611	5	54	theme	commercial	910:919	arg1	rheometer					921:929	a commercial rheometer	908:929	a commercial rheometer	908:929	Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively.					
30859611	6	55	theme	high	1039:1042	arg1	weight					1054:1059	high molecular weight HA	1039:1062	high molecular weight HA (>6.1MDa)	1039:1072	HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75.					
30859611	6	55	theme	high	1039:1042	arg1	>6.1MDa					1065:1071	>6.1MDa	1065:1071	>6.1MDa	1065:1071	HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75.					
30859611	7	56	theme	sham-operated	1243:1255	arg1	limbs					1257:1261	sham-operated limbs	1243:1261	sham-operated limbs	1243:1261	Lubricin concentrations increased after surgery to a greater extent in the OA as compared to sham-operated limbs.					
30859611	1	57	theme	articular	233:241	arg1	fracture					243:250	articular fracture	233:250	articular fracture	233:250	The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture.					
30859611	5	58	theme	Synovial	769:776	arg1	viscosity					784:792	Synovial fluid viscosity and cartilage friction coefficients	769:828	viscosity	784:792	Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively.					
30859611	10	59	theme	fluid	1681:1685	arg1	washout					1687:1693	synovial fluid washout	1672:1693	synovial fluid washout during arthroscopy	1672:1712	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	1	60	dep	fluid	189:193	arg1	composition					195:205	composition	195:205	composition	195:205	The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture.					
30859611	10	61	from	pronounced	1481:1490	arg1	limbs					1502:1506	the OA limbs	1495:1506	the OA limbs	1495:1506	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	2	62	theme	horses	375:380	arg1	joint					361:365	the middle carpal joint	343:365	the middle carpal joint of four horses	343:380	Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control.					
30859611	4	63	theme	sandwich	670:677	arg1	ELISAs					679:684	sandwich ELISAs	670:684	sandwich ELISAs	670:684	Lubricin and hyaluronic acid (HA) concentrations were measured using sandwich ELISAs, and the molecular weight distribution of HA was analyzed via gel electrophoresis.					
30859611	11	64	theme	2019	1717:1720	arg1	Research					1734:1741	© 2019 Orthopaedic Research	1715:1741	© 2019 Orthopaedic Research Society.	1715:1750	© 2019 Orthopaedic Research Society.					
30859611	5	65	theme	fluid	778:782	arg1	viscosity					784:792	Synovial fluid viscosity and cartilage friction coefficients	769:828	viscosity	784:792	Synovial fluid viscosity and cartilage friction coefficients across all modes of lubrication were measured on days 0, 19, 33, and 61 using a commercial rheometer and a custom tribometer, respectively.					
30859611	10	66	from	derangements	1517:1528	arg1	viscosity					1571:1579	viscosity	1571:1579	viscosity	1571:1579	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	10	66	from	derangements	1517:1528	arg1	distribution					1557:1568	HA molecular weight distribution	1537:1568	HA molecular weight distribution	1537:1568	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	10	66	from	derangements	1517:1528	arg1	HA					1533:1534	HA	1533:1534	HA	1533:1534	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	10	66	from	derangements	1517:1528	arg1	number					1597:1602	transition number	1586:1602	transition number	1586:1602	Although more pronounced in the OA limbs, similar derangements in HA, HA molecular weight distribution, viscosity, and transition number were observed in the sham-operated limbs, which may be explained by synovial fluid washout during arthroscopy.					
30859611	2	67	theme	Post-traumatic	253:266	arg1	osteoarthritis					268:281	Post-traumatic osteoarthritis	253:281	Post-traumatic osteoarthritis (PTOA)	253:288	Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control.					
30859611	2	67	theme	Post-traumatic	253:266	arg1	PTOA					284:287	PTOA	284:287	PTOA	284:287	Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral limb served as a sham-operated control.					
30859611	6	68	theme	pre-operative	1093:1105	arg1	values					1107:1112	pre-operative values	1093:1112	pre-operative values	1093:1112	HA concentrations were significantly decreased post-operatively, and high molecular weight HA (>6.1MDa) did not recover to pre-operative values by the study termination at day 75.					
30859611	7	69	theme	greater	1203:1209	arg1	extent					1211:1216	a greater extent	1201:1216	a greater extent in the OA	1201:1226	Lubricin concentrations increased after surgery to a greater extent in the OA as compared to sham-operated limbs.					
31822561	9	0	theme	glycosaminoglycan	1576:1592	arg1	hyaluronan					1594:1603	the glycosaminoglycan hyaluronan	1572:1603	the glycosaminoglycan hyaluronan	1572:1603	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	7	1	theme	knockout	1246:1253	arg1	brains					1255:1260	tenascin-R knockout brains	1235:1260	tenascin-R knockout brains	1235:1260	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	1	2	theme	interest	263:270	arg1	explosion					250:258	an explosion	247:258	an explosion of interest over the last decade	247:291	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	2	3	theme	adult-brain	472:482	arg1	plasticity					484:493	both developmental and adult-brain plasticity	449:493	plasticity	484:493	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	9	4	theme	distinct	1449:1456	arg1	other					1556:1560	other	1556:1560	other	1556:1560	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	9	4	theme	distinct	1449:1456	arg1	kinds					1458:1462	two distinct kinds	1445:1462	two distinct kinds of interactions of PNN components with the neuronal surface	1445:1522	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	9	4	theme	distinct	1449:1456	arg1	one					1525:1527	one	1525:1527	one	1525:1527	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	1	5	theme	extracellular	176:188	arg1	matrix					190:195	the extracellular matrix	172:195	the extracellular matrix of the central nervous system	172:225	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	9	6	theme	PNN	1483:1485	arg1	components					1487:1496	PNN components	1483:1496	PNN components	1483:1496	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	4	7	theme	molecular	768:776	arg1	composition					778:788	their molecular composition	762:788	their molecular composition	762:788	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	2	8	from	synapses	342:349	arg1	bodies					363:368	the cell bodies	354:368	the cell bodies of a subset of the neurons in the central nervous system	354:425	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	2	9	theme	reticulated	300:310	arg1	structures					312:321	These reticulated structures	294:321	These reticulated structures	294:321	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	3	10	from	demonstrations	552:565	arg1	structures					526:535	these structures	520:535	these structures	520:535	Despite the interest in these structures and compelling demonstrations of their importance in regulating plasticity, their precise functional mechanisms remain elusive.					
31822561	3	11	theme	importance	576:585	arg1	interest					508:515	the interest	504:515	the interest in these structures	504:535	Despite the interest in these structures and compelling demonstrations of their importance in regulating plasticity, their precise functional mechanisms remain elusive.					
31822561	3	11	theme	importance	576:585	arg1	demonstrations					552:565	compelling demonstrations	541:565	compelling demonstrations of their importance in regulating plasticity	541:610	Despite the interest in these structures and compelling demonstrations of their importance in regulating plasticity, their precise functional mechanisms remain elusive.					
31822561	4	12	theme	structure	794:802	arg1	knowledge					749:757	an incomplete knowledge	735:757	an incomplete knowledge of their molecular composition and structure	735:802	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	4	12	theme	structure	794:802	arg1	failure					810:816	a failure to identify PNN-specific targets	808:849	a failure to identify PNN-specific targets	808:849	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	10	13	theme	future	1713:1718	arg1	strategies					1720:1729	future strategies to specifically disrupt PNNs to precisely dissect their function	1713:1794	future strategies to specifically disrupt PNNs to precisely dissect their function	1713:1794	We propose that these findings offer important insight into PNN structure and lay important groundwork for future strategies to specifically disrupt PNNs to precisely dissect their function.					
31822561	1	14	theme	last	281:284	arg1	decade					286:291	the last decade	277:291	the last decade	277:291	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	4	15	theme	incomplete	738:747	arg1	knowledge					749:757	an incomplete knowledge	735:757	an incomplete knowledge of their molecular composition and structure	735:802	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	6	16	from	role	1018:1021	arg1	structure					1084:1092	PNN structure	1080:1092	PNN structure	1080:1092	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
31822561	1	17	theme	system	220:225	arg1	matrix					190:195	the extracellular matrix	172:195	the extracellular matrix of the central nervous system	172:225	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	7	18	dep	RPTPζ	1127:1131	arg1	the					1112:1114	the	1112:1114	the	1112:1114	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	7	18	dep	RPTPζ	1127:1131	arg1	absence					1116:1122	absence	1116:1122	absence	1116:1122	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	6	19	theme	receptor	1026:1033	arg1	phosphatase					1052:1062	receptor protein tyrosine phosphatase	1026:1062	receptor protein tyrosine phosphatase zeta (RPTPζ)	1026:1075	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
31822561	10	20	theme	important	1688:1696	arg1	groundwork					1698:1707	important groundwork	1688:1707	important groundwork for future strategies to specifically disrupt PNNs to precisely dissect their function	1688:1794	We propose that these findings offer important insight into PNN structure and lay important groundwork for future strategies to specifically disrupt PNNs to precisely dissect their function.					
31822561	2	21	theme	neurons	389:395	arg1	subset					375:380	a subset	373:380	a subset of the neurons in the central nervous system	373:425	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	0	22	theme	protein	4:10	arg1	critical					53:60	critical	53:60	critical	53:60	The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure.					
31822561	0	22	theme	protein	4:10	arg1	RPTPζ/phosphacan					33:48	The protein tyrosine phosphatase RPTPζ/phosphacan	0:48	The protein tyrosine phosphatase RPTPζ/phosphacan	0:48	The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure.					
31822561	3	23	from	interest	508:515	arg1	structures					526:535	these structures	520:535	these structures	520:535	Despite the interest in these structures and compelling demonstrations of their importance in regulating plasticity, their precise functional mechanisms remain elusive.					
31822561	1	24	theme	Perineuronal	94:105	arg1	nets					107:110	Perineuronal nets	94:110	Perineuronal nets (PNNs)	94:117	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	1	24	theme	Perineuronal	94:105	arg1	substructures					151:163	conspicuous neuron-specific substructures	123:163	conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade	123:291	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	1	24	theme	Perineuronal	94:105	arg1	PNNs					113:116	PNNs	113:116	PNNs	113:116	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	4	25	theme	PNNs	706:709	arg1	understanding					689:701	The limited mechanistic understanding	665:701	The limited mechanistic understanding of PNNs	665:709	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	2	26	theme	key	436:438	arg1	roles					440:444	key roles	436:444	key roles	436:444	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	2	27	from	subset	375:380	arg1	system					420:425	the central nervous system	400:425	the central nervous system	400:425	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	1	28	theme	central	204:210	arg1	system					220:225	the central nervous system	200:225	the central nervous system	200:225	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	0	29	theme	phosphatase	21:31	arg1	critical					53:60	critical	53:60	critical	53:60	The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure.					
31822561	0	29	theme	phosphatase	21:31	arg1	RPTPζ/phosphacan					33:48	The protein tyrosine phosphatase RPTPζ/phosphacan	0:48	The protein tyrosine phosphatase RPTPζ/phosphacan	0:48	The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure.					
31822561	8	30	theme	RPTPζ	1322:1326	arg1	contribution					1306:1317	the contribution	1302:1317	the contribution of RPTPζ to PNN formation and structure, which enabled us to generate a more detailed model for PNNs	1302:1418	Furthermore, we biochemically analyzed the contribution of RPTPζ to PNN formation and structure, which enabled us to generate a more detailed model for PNNs.					
31822561	9	31	with	interactions	1467:1478	arg1	surface					1516:1522	the neuronal surface	1503:1522	the neuronal surface	1503:1522	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	10	32	theme	important	1643:1651	arg1	insight					1653:1659	important insight	1643:1659	important insight into PNN structure	1643:1678	We propose that these findings offer important insight into PNN structure and lay important groundwork for future strategies to specifically disrupt PNNs to precisely dissect their function.					
31822561	8	33	theme	detailed	1396:1403	arg1	model					1405:1409	a more detailed model	1389:1409	a more detailed model for PNNs	1389:1418	Furthermore, we biochemically analyzed the contribution of RPTPζ to PNN formation and structure, which enabled us to generate a more detailed model for PNNs.					
31822561	1	34	theme	nervous	212:218	arg1	system					220:225	the central nervous system	200:225	the central nervous system	200:225	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	0	35	theme	tyrosine	12:19	arg1	critical					53:60	critical	53:60	critical	53:60	The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure.					
31822561	0	35	theme	tyrosine	12:19	arg1	RPTPζ/phosphacan					33:48	The protein tyrosine phosphatase RPTPζ/phosphacan	0:48	The protein tyrosine phosphatase RPTPζ/phosphacan	0:48	The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure.					
31822561	4	36	theme	composition	778:788	arg1	knowledge					749:757	an incomplete knowledge	735:757	an incomplete knowledge of their molecular composition and structure	735:802	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	4	36	theme	composition	778:788	arg1	failure					810:816	a failure to identify PNN-specific targets	808:849	a failure to identify PNN-specific targets	808:849	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	2	37	theme	nervous	412:418	arg1	system					420:425	the central nervous system	400:425	the central nervous system	400:425	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	4	38	theme	limited	669:675	arg1	understanding					689:701	The limited mechanistic understanding	665:701	The limited mechanistic understanding of PNNs	665:709	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	8	39	theme	PNN	1331:1333	arg1	formation					1335:1343	PNN formation	1331:1343	PNN formation	1331:1343	Furthermore, we biochemically analyzed the contribution of RPTPζ to PNN formation and structure, which enabled us to generate a more detailed model for PNNs.					
31822561	6	40	theme	PNN	1080:1082	arg1	structure					1084:1092	PNN structure	1080:1092	PNN structure	1080:1092	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
31822561	2	41	theme	central	404:410	arg1	system					420:425	the central nervous system	400:425	the central nervous system	400:425	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	2	42	theme	developmental	454:466	arg1	plasticity					484:493	both developmental and adult-brain plasticity	449:493	plasticity	484:493	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	6	43	theme	neuronal	982:989	arg1	cultures					991:998	neuronal cultures	982:998	neuronal cultures	982:998	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
31822561	7	44	theme	PNNs	1161:1164	arg1	structure					1148:1156	the reticular structure	1134:1156	the reticular structure of PNNs	1134:1164	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	6	45	theme	mouse	965:969	arg1	models					971:976	mouse models	965:976	mouse models	965:976	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
31822561	7	46	theme	tenascin-R	1235:1244	arg1	brains					1255:1260	tenascin-R knockout brains	1235:1260	tenascin-R knockout brains	1235:1260	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	9	47	theme	interactions	1467:1478	arg1	other					1556:1560	other	1556:1560	other	1556:1560	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	9	47	theme	interactions	1467:1478	arg1	kinds					1458:1462	two distinct kinds	1445:1462	two distinct kinds of interactions of PNN components with the neuronal surface	1445:1522	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	9	47	theme	interactions	1467:1478	arg1	one					1525:1527	one	1525:1527	one	1525:1527	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	2	48	theme	cell	358:361	arg1	bodies					363:368	the cell bodies	354:368	the cell bodies of a subset of the neurons in the central nervous system	354:425	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	7	49	theme	reticular	1138:1146	arg1	structure					1148:1156	the reticular structure	1134:1156	the reticular structure of PNNs	1134:1164	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	7	50	theme	structural	1198:1207	arg1	abnormalities					1209:1221	the PNN structural abnormalities	1190:1221	the PNN structural abnormalities observed in tenascin-R knockout brains	1190:1260	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	9	51	theme	components	1487:1496	arg1	interactions					1467:1478	interactions	1467:1478	interactions of PNN components with the neuronal surface	1467:1522	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	4	52	theme	PNN-specific	830:841	arg1	targets					843:849	PNN-specific targets	830:849	PNN-specific targets	830:849	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	2	53	from	neurons	389:395	arg1	system					420:425	the central nervous system	400:425	the central nervous system	400:425	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	1	54	theme	conspicuous	123:133	arg1	nets					107:110	Perineuronal nets	94:110	Perineuronal nets (PNNs)	94:117	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	1	54	theme	conspicuous	123:133	arg1	substructures					151:163	conspicuous neuron-specific substructures	123:163	conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade	123:291	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	7	55	located	observed	1223:1230	arg1	brains					1255:1260	tenascin-R knockout brains	1235:1260	tenascin-R knockout brains	1235:1260	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	7	55	located	observed	1223:1230	arg2	abnormalities					1209:1221	the PNN structural abnormalities	1190:1221	the PNN structural abnormalities observed in tenascin-R knockout brains	1190:1260	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	6	56	theme	zeta	1064:1067	arg1	role					1018:1021	a role	1016:1021	a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure	1016:1092	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
31822561	7	57	dep	structural	1198:1207	arg1	PNN					1194:1196	PNN	1194:1196	PNN	1194:1196	We found that in the absence of RPTPζ, the reticular structure of PNNs is lost and phenocopies the PNN structural abnormalities observed in tenascin-R knockout brains.					
31822561	3	58	theme	compelling	541:550	arg1	demonstrations					552:565	compelling demonstrations	541:565	compelling demonstrations of their importance in regulating plasticity	541:610	Despite the interest in these structures and compelling demonstrations of their importance in regulating plasticity, their precise functional mechanisms remain elusive.					
31822561	1	59	theme	neuron-specific	135:149	arg1	nets					107:110	Perineuronal nets	94:110	Perineuronal nets (PNNs)	94:117	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	1	59	theme	neuron-specific	135:149	arg1	substructures					151:163	conspicuous neuron-specific substructures	123:163	conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade	123:291	Perineuronal nets (PNNs) are conspicuous neuron-specific substructures within the extracellular matrix of the central nervous system that have generated an explosion of interest over the last decade.					
31822561	6	60	theme	phosphatase	1052:1062	arg1	RPTPζ					1070:1074	RPTPζ	1070:1074	RPTPζ	1070:1074	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
31822561	6	60	theme	phosphatase	1052:1062	arg1	zeta					1064:1067	receptor protein tyrosine phosphatase zeta	1026:1067	receptor protein tyrosine phosphatase zeta (RPTPζ)	1026:1075	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
31822561	0	61	theme	net	79:81	arg1	structure					83:91	perineuronal net structure	66:91	perineuronal net structure	66:91	The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure.					
31822561	10	62	theme	PNN	1666:1668	arg1	structure					1670:1678	PNN structure	1666:1678	PNN structure	1666:1678	We propose that these findings offer important insight into PNN structure and lay important groundwork for future strategies to specifically disrupt PNNs to precisely dissect their function.					
31822561	3	63	theme	precise	619:625	arg1	mechanisms					638:647	their precise functional mechanisms	613:647	their precise functional mechanisms	613:647	Despite the interest in these structures and compelling demonstrations of their importance in regulating plasticity, their precise functional mechanisms remain elusive.					
31822561	9	64	theme	neuronal	1507:1514	arg1	surface					1516:1522	the neuronal surface	1503:1522	the neuronal surface	1503:1522	We provide evidence for two distinct kinds of interactions of PNN components with the neuronal surface, one dependent on RPTPζ and the other requiring the glycosaminoglycan hyaluronan.					
31822561	2	65	theme	subset	375:380	arg1	bodies					363:368	the cell bodies	354:368	the cell bodies of a subset of the neurons in the central nervous system	354:425	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	4	66	theme	mechanistic	677:687	arg1	understanding					689:701	The limited mechanistic understanding	665:701	The limited mechanistic understanding of PNNs	665:709	The limited mechanistic understanding of PNNs is primarily because of an incomplete knowledge of their molecular composition and structure and a failure to identify PNN-specific targets.					
31822561	6	67	theme	tyrosine	1043:1050	arg1	phosphatase					1052:1062	receptor protein tyrosine phosphatase	1026:1062	receptor protein tyrosine phosphatase zeta (RPTPζ)	1026:1075	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
31822561	0	68	theme	perineuronal	66:77	arg1	structure					83:91	perineuronal net structure	66:91	perineuronal net structure	66:91	The protein tyrosine phosphatase RPTPζ/phosphacan is critical for perineuronal net structure.					
31822561	3	69	theme	functional	627:636	arg1	mechanisms					638:647	their precise functional mechanisms	613:647	their precise functional mechanisms	613:647	Despite the interest in these structures and compelling demonstrations of their importance in regulating plasticity, their precise functional mechanisms remain elusive.					
31822561	2	70	from	system	420:425	arg1	subset					375:380	a subset	373:380	a subset of the neurons in the central nervous system	373:425	These reticulated structures appear to surround synapses on the cell bodies of a subset of the neurons in the central nervous system and play key roles in both developmental and adult-brain plasticity.					
31822561	6	71	theme	protein	1035:1041	arg1	phosphatase					1052:1062	receptor protein tyrosine phosphatase	1026:1062	receptor protein tyrosine phosphatase zeta (RPTPζ)	1026:1075	Here, using mouse models and neuronal cultures, we demonstrate a role of receptor protein tyrosine phosphatase zeta (RPTPζ) in PNN structure.					
30886367	5	0	theme	meal	768:771	arg1	pH					808:809	a constant pH	797:809	a constant pH for each step of digestion	797:836	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	5	0	theme	meal	768:771	arg1	ratios					758:763	constant ratios	749:763	constant ratios of meal to digestive fluids	749:791	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	9	1	theme	micronutrients	1546:1559	arg1	release					1535:1541	the release	1531:1541	the release of micronutrients from the food matrix	1531:1580	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	7	2	theme	intestinal	1005:1014	arg1	digestion					1016:1024	sequential oral, gastric and intestinal digestion	976:1024	sequential oral, gastric and intestinal digestion	976:1024	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	10	3	theme	~7	1622:1623	arg1	d					1625:1625	~7 d	1622:1625	~7 d	1622:1625	The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.					
30886367	4	4	theme	standard	591:598	arg1	equipment					611:619	standard laboratory equipment	591:619	standard laboratory equipment	591:619	The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it.					
30886367	6	5	theme	suitable	883:890	arg1	method					854:859	the method	850:859	the method simple to use but not suitable for simulating digestion kinetics	850:924	This makes the method simple to use but not suitable for simulating digestion kinetics.					
30886367	4	6	used	used	581:584	arg2	method					556:561	The method	552:561	The method	552:561	The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it.					
30886367	8	7	theme	improved	1175:1182	arg1	INFOGEST					1202:1209	INFOGEST 2.0	1202:1213	INFOGEST 2.0	1202:1213	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	8	7	theme	improved	1175:1182	arg1	method					1194:1199	This amended and improved digestion method	1158:1199	This amended and improved digestion method (INFOGEST 2.0)	1158:1214	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	1	8	theme	human	162:166	arg1	health					168:173	human health	162:173	human health	162:173	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	9	9	theme	digestion	1440:1448	arg1	products					1450:1457	the digestion products	1436:1457	the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars)	1436:1514	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	7	10	theme	food	946:949	arg1	samples					951:957	food samples	946:957	food samples	946:957	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	9	11	theme	peptides/amino	1466:1479	arg1	acids					1481:1485	peptides/amino acids	1466:1485	peptides/amino acids	1466:1485	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	9	11	theme	peptides/amino	1466:1479	arg1	acids					1494:1498	fatty acids	1488:1498	fatty acids	1488:1498	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	9	11	theme	peptides/amino	1466:1479	arg1	sugars					1508:1513	simple sugars	1501:1513	simple sugars	1501:1513	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	10	12	theme	~5	1638:1639	arg1	d					1641:1641	~5 d	1638:1641	~5 d required for the determination of enzyme activities	1638:1693	The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.					
30886367	9	13	dep	products	1450:1457	arg1	acids					1481:1485	peptides/amino acids	1466:1485	peptides/amino acids	1466:1485	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	9	13	dep	products	1450:1457	arg1	acids					1494:1498	fatty acids	1488:1498	fatty acids	1488:1498	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	9	13	dep	products	1450:1457	arg1	sugars					1508:1513	simple sugars	1501:1513	simple sugars	1501:1513	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	7	14	theme	gastric	993:999	arg1	digestion					1016:1024	sequential oral, gastric and intestinal digestion	976:1024	sequential oral, gastric and intestinal digestion	976:1024	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	4	15	theme	laboratory	600:609	arg1	equipment					611:619	standard laboratory equipment	591:619	standard laboratory equipment	591:619	The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it.					
30886367	7	16	theme	physiological	1138:1150	arg1	data					1152:1155	available physiological data	1128:1155	available physiological data	1128:1155	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	2	17	theme	physiological	352:364	arg1	relevance					366:374	very little physiological relevance	340:374	very little physiological relevance	340:374	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	1	18	theme	Developing	72:81	arg1	understanding					97:109	a mechanistic understanding	83:109	Developing a mechanistic understanding of the impact of food structure and composition on human health	72:173	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	5	19	theme	constant	749:756	arg1	ratios					758:763	constant ratios	749:763	constant ratios of meal to digestive fluids	749:791	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	2	20	theme	meaningful	398:407	arg1	comparison					409:418	the meaningful comparison	394:418	the meaningful comparison of results	394:429	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	5	21	theme	constant	799:806	arg1	pH					808:809	a constant pH	797:809	a constant pH for each step of digestion	797:836	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	6	22	theme	simple	861:866	arg1	method					854:859	the method	850:859	the method simple to use but not suitable for simulating digestion kinetics	850:924	This makes the method simple to use but not suitable for simulating digestion kinetics.					
30886367	1	23	theme	mechanistic	85:95	arg1	understanding					97:109	a mechanistic understanding	83:109	Developing a mechanistic understanding of the impact of food structure and composition on human health	72:173	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	2	24	used	used	282:285	arg2	simulations					265:275	These simulations	259:275	These simulations	259:275	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	3	25	theme	standardized	436:447	arg1	protocol					449:456	The standardized protocol	432:456	The standardized protocol presented here	432:471	The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network.					
30886367	7	26	theme	available	1128:1136	arg1	data					1152:1155	available physiological data	1128:1155	available physiological data	1128:1155	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	9	27	theme	fatty	1488:1492	arg1	acids					1481:1485	peptides/amino acids	1466:1485	peptides/amino acids	1466:1485	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	9	27	theme	fatty	1488:1492	arg1	acids					1494:1498	fatty acids	1488:1498	fatty acids	1488:1498	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	1	28	from	impact	118:123	arg1	health					168:173	human health	162:173	human health	162:173	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	8	29	theme	phase	1305:1309	arg1	inclusion					1283:1291	the inclusion	1279:1291	the inclusion of the oral phase	1279:1309	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	8	29	theme	phase	1305:1309	arg1	use					1319:1321	the use	1315:1321	the use of gastric lipase	1315:1339	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	8	30	theme	oral	1300:1303	arg1	phase					1305:1309	the oral phase	1296:1309	the oral phase	1296:1309	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	4	31	theme	researchers	682:692	arg1	range					673:677	a wide range	666:677	a wide range of researchers	666:692	The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it.					
30886367	5	32	theme	digestion	828:836	arg1	step					820:823	each step	815:823	each step of digestion	815:836	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	10	33	theme	enzyme	1677:1682	arg1	activities					1684:1693	enzyme activities	1677:1693	enzyme activities	1677:1693	The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.					
30886367	2	34	theme	conditions	313:322	arg1	range					294:298	a wide range	287:298	a wide range of different conditions that often have very little physiological relevance	287:374	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	9	35	theme	simple	1501:1506	arg1	acids					1481:1485	peptides/amino acids	1466:1485	peptides/amino acids	1466:1485	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	9	35	theme	simple	1501:1506	arg1	sugars					1508:1513	simple sugars	1501:1513	simple sugars	1501:1513	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	10	36	theme	whole	1587:1591	arg1	protocol					1593:1600	The whole protocol	1583:1600	The whole protocol	1583:1600	The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.					
30886367	0	37	theme	in	16:17	arg1	simulation					25:34	in vitro simulation	16:34	in vitro simulation of gastrointestinal food digestion	16:69	INFOGEST static in vitro simulation of gastrointestinal food digestion.					
30886367	9	38	from	matrix	1575:1580	arg1	release					1535:1541	the release	1531:1541	the release of micronutrients from the food matrix	1531:1580	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	2	39	theme	wide	289:292	arg1	range					294:298	a wide range	287:298	a wide range of different conditions that often have very little physiological relevance	287:374	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	2	40	theme	different	303:311	arg1	conditions					313:322	different conditions	303:322	different conditions that often have very little physiological relevance	303:374	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	5	41	theme	digestive	776:784	arg1	fluids					786:791	digestive fluids	776:791	digestive fluids	776:791	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	9	42	theme	foods	1417:1421	arg1	digestion					1404:1412	digestion	1404:1412	digestion of foods	1404:1421	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	5	43	theme	static	715:720	arg1	method					732:737	a static digestion method	713:737	a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion	713:836	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	5	43	theme	static	715:720	arg1	It					707:708	It	707:708	It	707:708	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	4	44	theme	wide	668:671	arg1	range					673:677	a wide range	666:677	a wide range of researchers	666:692	The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it.					
30886367	8	45	theme	gastric	1326:1332	arg1	lipase					1334:1339	gastric lipase	1326:1339	gastric lipase	1326:1339	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	9	46	used	used	1360:1363	arg2	method					1346:1351	The method	1342:1351	The method	1342:1351	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	2	47	theme	results	423:429	arg1	comparison					409:418	the meaningful comparison	394:418	the meaningful comparison of results	394:429	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	7	48	theme	digestion	1105:1113	arg1	dilution					1080:1087	dilution	1080:1087	dilution	1080:1087	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	7	48	theme	digestion	1105:1113	arg1	pH					1090:1091	pH	1090:1091	pH	1090:1091	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	7	48	theme	digestion	1105:1113	arg1	enzymes					1065:1071	enzymes	1065:1071	enzymes	1065:1071	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	7	48	theme	digestion	1105:1113	arg1	bile					1074:1077	bile	1074:1077	bile	1074:1077	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	7	48	theme	digestion	1105:1113	arg1	time					1097:1100	time	1097:1100	time	1097:1100	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	7	48	theme	digestion	1105:1113	arg1	electrolytes					1051:1062	electrolytes	1051:1062	electrolytes	1051:1062	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	5	49	theme	digestion	722:730	arg1	method					732:737	a static digestion method	713:737	a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion	713:836	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	5	49	theme	digestion	722:730	arg1	It					707:708	It	707:708	It	707:708	It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion.					
30886367	9	50	dep	acids	1481:1485	arg1	e.g.					1460:1463	e.g.	1460:1463	e.g.	1460:1463	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	8	51	theme	original	1254:1261	arg1	method					1263:1268	the original method	1250:1268	the original method	1250:1268	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	0	52	theme	food	56:59	arg1	digestion					61:69	gastrointestinal food digestion	39:69	gastrointestinal food digestion	39:69	INFOGEST static in vitro simulation of gastrointestinal food digestion.					
30886367	8	53	theme	amended	1163:1169	arg1	INFOGEST					1202:1209	INFOGEST 2.0	1202:1213	INFOGEST 2.0	1202:1213	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	8	53	theme	amended	1163:1169	arg1	method					1194:1199	This amended and improved digestion method	1158:1199	This amended and improved digestion method (INFOGEST 2.0)	1158:1214	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	10	54	theme	activities	1684:1693	arg1	determination					1660:1672	the determination	1656:1672	the determination of enzyme activities	1656:1693	The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.					
30886367	7	55	theme	sequential	976:985	arg1	digestion					1016:1024	sequential oral, gastric and intestinal digestion	976:1024	sequential oral, gastric and intestinal digestion	976:1024	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	1	56	theme	impact	118:123	arg1	understanding					97:109	a mechanistic understanding	83:109	Developing a mechanistic understanding of the impact of food structure and composition on human health	72:173	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	0	57	theme	gastrointestinal	39:54	arg1	digestion					61:69	gastrointestinal food digestion	39:69	gastrointestinal food digestion	39:69	INFOGEST static in vitro simulation of gastrointestinal food digestion.					
30886367	3	58	theme	international	488:500	arg1	consensus					502:510	an international consensus	485:510	an international consensus developed by the COST INFOGEST network	485:549	The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network.					
30886367	8	59	theme	lipase	1334:1339	arg1	inclusion					1283:1291	the inclusion	1279:1291	the inclusion of the oral phase	1279:1309	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	8	59	theme	lipase	1334:1339	arg1	use					1319:1321	the use	1315:1321	the use of gastric lipase	1315:1339	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	3	60	theme	COST	529:532	arg1	network					543:549	the COST INFOGEST network	525:549	the COST INFOGEST network	525:549	The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network.					
30886367	2	61	theme	little	345:350	arg1	relevance					366:374	very little physiological relevance	340:374	very little physiological relevance	340:374	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	6	62	theme	digestion	907:915	arg1	kinetics					917:924	digestion kinetics	907:924	digestion kinetics	907:924	This makes the method simple to use but not suitable for simulating digestion kinetics.					
30886367	4	63	dep	limited	634:640	arg1	experience					642:651	experience	642:651	experience to encourage a wide range of researchers to adopt it	642:704	The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it.					
30886367	1	64	from	digestion	212:220	arg1	tract					252:256	the upper gastrointestinal tract	225:256	the upper gastrointestinal tract	225:256	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	1	65	theme	food	128:131	arg1	structure					133:141	food structure	128:141	food structure	128:141	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	1	66	theme	upper	229:233	arg1	tract					252:256	the upper gastrointestinal tract	225:256	the upper gastrointestinal tract	225:256	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	7	67	theme	oral	987:990	arg1	digestion					1016:1024	sequential oral, gastric and intestinal digestion	976:1024	sequential oral, gastric and intestinal digestion	976:1024	Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data.					
30886367	9	68	theme	food	1570:1573	arg1	matrix					1575:1580	the food matrix	1566:1580	the food matrix	1566:1580	The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix.					
30886367	0	69	dep	in	16:17	arg1	vitro					19:23	vitro	19:23	vitro	19:23	INFOGEST static in vitro simulation of gastrointestinal food digestion.					
30886367	1	70	theme	structure	133:141	arg1	impact					118:123	the impact	114:123	the impact of food structure and composition on human health	114:173	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	8	71	theme	digestion	1184:1192	arg1	INFOGEST					1202:1209	INFOGEST 2.0	1202:1213	INFOGEST 2.0	1202:1213	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	8	71	theme	digestion	1184:1192	arg1	method					1194:1199	This amended and improved digestion method	1158:1199	This amended and improved digestion method (INFOGEST 2.0)	1158:1214	This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase.					
30886367	1	72	theme	gastrointestinal	235:250	arg1	tract					252:256	the upper gastrointestinal tract	225:256	the upper gastrointestinal tract	225:256	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	3	73	theme	INFOGEST	534:541	arg1	network					543:549	the COST INFOGEST network	525:549	the COST INFOGEST network	525:549	The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network.					
30886367	1	74	from	understanding	97:109	arg1	health					168:173	human health	162:173	human health	162:173	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	0	75	theme	digestion	61:69	arg1	simulation					25:34	in vitro simulation	16:34	in vitro simulation of gastrointestinal food digestion	16:69	INFOGEST static in vitro simulation of gastrointestinal food digestion.					
30886367	2	76	contain	have	335:338	arg1	conditions					313:322	different conditions	303:322	different conditions that often have very little physiological relevance	303:374	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	2	76	contain	have	335:338	arg2	relevance					366:374	very little physiological relevance	340:374	very little physiological relevance	340:374	These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results.					
30886367	1	77	from	health	168:173	arg1	understanding					97:109	a mechanistic understanding	83:109	Developing a mechanistic understanding of the impact of food structure and composition on human health	72:173	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
30886367	1	78	theme	composition	147:157	arg1	impact					118:123	the impact	114:123	the impact of food structure and composition on human health	114:173	Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract.					
31393229	0	0	theme	bone	104:107	arg1	engineering					116:126	bone tissue engineering	104:126	bone tissue engineering	104:126	Development of chitosan/gelatin hydrogels incorporation of biphasic calcium phosphate nanoparticles for bone tissue engineering.					
31393229	6	1	theme	CCK-8	1047:1051	arg1	analysis					1053:1060	CCK-8 analysis	1047:1060	CCK-8 analysis	1047:1060	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	8	2	theme	prepared	1462:1469	arg1	nanocomposites					1475:1488	prepared CGB nanocomposites	1462:1488	prepared CGB nanocomposites	1462:1488	The above results suggested that prepared CGB nanocomposites had the potential to be applied in bone tissue engineering.					
31393229	1	3	theme	phosphate	194:202	arg1	BCP-NPs					219:225	BCP-NPs	219:225	BCP-NPs	219:225	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article.					
31393229	1	3	theme	phosphate	194:202	arg1	nanoparticles					204:216	biphasic calcium phosphate nanoparticles	177:216	biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering	177:272	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article.					
31393229	5	4	theme	CGB	977:979	arg1	scaffolds					981:989	CGB scaffolds	977:989	CGB scaffolds	977:989	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	6	5	theme	mesenchymal	1167:1177	arg1	BMSCs					1191:1195	BMSCs	1191:1195	BMSCs	1191:1195	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	6	5	theme	mesenchymal	1167:1177	arg1	cells					1184:1188	mesenchymal stem cells	1167:1188	bone marrow mesenchymal stem cells (BMSCs)	1155:1196	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	8	6	theme	tissue	1530:1535	arg1	engineering					1537:1547	bone tissue engineering	1525:1547	bone tissue engineering	1525:1547	The above results suggested that prepared CGB nanocomposites had the potential to be applied in bone tissue engineering.					
31393229	7	7	theme	necrotic	1388:1395	arg1	lesions					1397:1403	large necrotic lesions	1382:1403	large necrotic lesions of rabbit femoral head	1382:1426	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	2	8	theme	nanocomposite	309:321	arg1	hydrogels					323:331	Such nanocomposite hydrogels	304:331	Such nanocomposite hydrogels	304:331	Such nanocomposite hydrogels were fabricated by using cycled freeze-thawing method, of which physicochemical and biological properties were regulated by adjusting the weight ratio of chitosan/gelatin/BCP-NPs.					
31393229	0	9	theme	tissue	109:114	arg1	engineering					116:126	bone tissue engineering	104:126	bone tissue engineering	104:126	Development of chitosan/gelatin hydrogels incorporation of biphasic calcium phosphate nanoparticles for bone tissue engineering.					
31393229	4	10	theme	strength	822:829	arg1	scaffolds					838:846	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds	764:846	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds	764:846	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	2	11	theme	Such	304:307	arg1	hydrogels					323:331	Such nanocomposite hydrogels	304:331	Such nanocomposite hydrogels	304:331	Such nanocomposite hydrogels were fabricated by using cycled freeze-thawing method, of which physicochemical and biological properties were regulated by adjusting the weight ratio of chitosan/gelatin/BCP-NPs.					
31393229	6	12	theme	bone	1155:1158	arg1	marrow					1160:1165	bone marrow mesenchymal stem cells (BMSCs)	1155:1196	bone marrow mesenchymal stem cells (BMSCs)	1155:1196	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	3	13	dep	Fourier	706:712	arg1	Transform					714:722	Transform	714:722	Transform Infrared Spectroscopy	714:744	The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis.					
31393229	6	14	theme	prepared	1081:1088	arg1	scaffolds					1090:1098	the prepared scaffolds	1077:1098	the prepared scaffolds	1077:1098	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	5	15	theme	body	944:947	arg1	SBF					957:959	SBF	957:959	SBF	957:959	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	5	15	theme	body	944:947	arg1	fluids					949:954	simulated body fluids	934:954	simulated body fluids (SBF)	934:960	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	7	16	theme	animal	1216:1221	arg1	experiments					1223:1233	In vivo animal experiments	1208:1233	In vivo animal experiments	1208:1233	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	4	17	theme	CGB	834:836	arg1	ratio					799:803	equilibrium swelling ratio	778:803	equilibrium swelling ratio	778:803	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	4	17	theme	CGB	834:836	arg1	strength					822:829	compressive strength	810:829	compressive strength of CGB	810:836	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	4	17	theme	CGB	834:836	arg1	porosity					768:775	porosity	768:775	porosity	768:775	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	5	18	theme	simulated	934:942	arg1	SBF					957:959	SBF	957:959	SBF	957:959	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	5	18	theme	simulated	934:942	arg1	fluids					949:954	simulated body fluids	934:954	simulated body fluids (SBF)	934:960	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	2	19	theme	chitosan/gelatin/BCP-NPs	487:510	arg1	ratio					478:482	the weight ratio	467:482	the weight ratio of chitosan/gelatin/BCP-NPs	467:510	Such nanocomposite hydrogels were fabricated by using cycled freeze-thawing method, of which physicochemical and biological properties were regulated by adjusting the weight ratio of chitosan/gelatin/BCP-NPs.					
31393229	7	20	theme	new	1249:1251	arg1	tissue					1258:1263	new bone tissue	1249:1263	new bone tissue	1249:1263	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	7	21	theme	bone	1253:1256	arg1	tissue					1258:1263	new bone tissue	1249:1263	new bone tissue	1249:1263	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	2	22	theme	biological	417:426	arg1	properties					428:437	physicochemical and biological properties	397:437	physicochemical and biological properties	397:437	Such nanocomposite hydrogels were fabricated by using cycled freeze-thawing method, of which physicochemical and biological properties were regulated by adjusting the weight ratio of chitosan/gelatin/BCP-NPs.					
31393229	7	23	theme	In	1208:1209	arg1	experiments					1223:1233	In vivo animal experiments	1208:1233	In vivo animal experiments	1208:1233	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	4	24	theme	ratio	799:803	arg1	scaffolds					838:846	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds	764:846	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds	764:846	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	5	25	dep	In	901:902	arg1	vitro					904:908	vitro	904:908	vitro	904:908	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	4	26	theme	compressive	810:820	arg1	strength					822:829	compressive strength	810:829	compressive strength of CGB	810:836	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	4	27	theme	BCP-NPs	878:884	arg1	concentration					886:898	the BCP-NPs concentration	874:898	the BCP-NPs concentration	874:898	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	3	28	theme	needle-like	517:527	arg1	BCP-NPs					529:535	The needle-like BCP-NPs	513:535	The needle-like BCP-NPs	513:535	The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis.					
31393229	2	29	theme	weight	471:476	arg1	ratio					478:482	the weight ratio	467:482	the weight ratio of chitosan/gelatin/BCP-NPs	467:510	Such nanocomposite hydrogels were fabricated by using cycled freeze-thawing method, of which physicochemical and biological properties were regulated by adjusting the weight ratio of chitosan/gelatin/BCP-NPs.					
31393229	3	30	theme	Fourier	706:712	arg1	analysis					754:761	Fourier Transform Infrared Spectroscopy (FT-IR) analysis	706:761	Fourier Transform Infrared Spectroscopy (FT-IR) analysis	706:761	The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis.					
31393229	1	31	theme	biphasic	177:184	arg1	BCP-NPs					219:225	BCP-NPs	219:225	BCP-NPs	219:225	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article.					
31393229	1	31	theme	biphasic	177:184	arg1	nanoparticles					204:216	biphasic calcium phosphate nanoparticles	177:216	biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering	177:272	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article.					
31393229	4	32	theme	porosity	768:775	arg1	scaffolds					838:846	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds	764:846	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds	764:846	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	8	33	theme	CGB	1471:1473	arg1	nanocomposites					1475:1488	prepared CGB nanocomposites	1462:1488	prepared CGB nanocomposites	1462:1488	The above results suggested that prepared CGB nanocomposites had the potential to be applied in bone tissue engineering.					
31393229	3	34	theme	Scanning	660:667	arg1	SEM					690:692	SEM	690:692	SEM	690:692	The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis.					
31393229	3	34	theme	Scanning	660:667	arg1	Microscope					678:687	Scanning Electron Microscope	660:687	Scanning Electron Microscope (SEM) images	660:700	The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis.					
31393229	5	35	theme	degradation	910:920	arg1	analysis					922:929	In vitro degradation analysis	901:929	In vitro degradation analysis in simulated body fluids (SBF)	901:960	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	3	36	theme	Electron	669:676	arg1	SEM					690:692	SEM	690:692	SEM	690:692	The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis.					
31393229	3	36	theme	Electron	669:676	arg1	Microscope					678:687	Scanning Electron Microscope	660:687	Scanning Electron Microscope (SEM) images	660:700	The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis.					
31393229	0	37	theme	calcium	68:74	arg1	nanoparticles					86:98	biphasic calcium phosphate nanoparticles	59:98	biphasic calcium phosphate nanoparticles	59:98	Development of chitosan/gelatin hydrogels incorporation of biphasic calcium phosphate nanoparticles for bone tissue engineering.					
31393229	8	38	theme	above	1433:1437	arg1	results					1439:1445	The above results	1429:1445	The above results	1429:1445	The above results suggested that prepared CGB nanocomposites had the potential to be applied in bone tissue engineering.					
31393229	5	39	from	analysis	922:929	arg1	SBF					957:959	SBF	957:959	SBF	957:959	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	5	39	from	analysis	922:929	arg1	fluids					949:954	simulated body fluids	934:954	simulated body fluids (SBF)	934:960	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	1	40	theme	chitosan/gelatin	133:148	arg1	hydrogel					150:157	The chitosan/gelatin hydrogel	129:157	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering	129:272	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article.					
31393229	0	41	theme	biphasic	59:66	arg1	nanoparticles					86:98	biphasic calcium phosphate nanoparticles	59:98	biphasic calcium phosphate nanoparticles	59:98	Development of chitosan/gelatin hydrogels incorporation of biphasic calcium phosphate nanoparticles for bone tissue engineering.					
31393229	8	42	theme	bone	1525:1528	arg1	engineering					1537:1547	bone tissue engineering	1525:1547	bone tissue engineering	1525:1547	The above results suggested that prepared CGB nanocomposites had the potential to be applied in bone tissue engineering.					
31393229	7	43	theme	rabbit	1408:1413	arg1	head					1423:1426	rabbit femoral head	1408:1426	rabbit femoral head	1408:1426	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	7	44	theme	CGB	1363:1365	arg1	scaffolds					1367:1375	CGB scaffolds	1363:1375	CGB scaffolds	1363:1375	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	2	45	theme	freeze-thawing	365:378	arg1	method					380:385	cycled freeze-thawing method	358:385	cycled freeze-thawing method	358:385	Such nanocomposite hydrogels were fabricated by using cycled freeze-thawing method, of which physicochemical and biological properties were regulated by adjusting the weight ratio of chitosan/gelatin/BCP-NPs.					
31393229	7	46	dep	In	1208:1209	arg1	vivo					1211:1214	vivo	1211:1214	vivo	1211:1214	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	7	47	theme	large	1382:1386	arg1	lesions					1397:1403	large necrotic lesions	1382:1403	large necrotic lesions of rabbit femoral head	1382:1426	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	6	48	dep	marrow	1160:1165	arg1	BMSCs					1191:1195	BMSCs	1191:1195	BMSCs	1191:1195	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	6	48	dep	marrow	1160:1165	arg1	cells					1184:1188	mesenchymal stem cells	1167:1188	bone marrow mesenchymal stem cells (BMSCs)	1155:1196	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	7	49	theme	femoral	1415:1421	arg1	head					1423:1426	rabbit femoral head	1408:1426	rabbit femoral head	1408:1426	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	2	50	theme	cycled	358:363	arg1	method					380:385	cycled freeze-thawing method	358:385	cycled freeze-thawing method	358:385	Such nanocomposite hydrogels were fabricated by using cycled freeze-thawing method, of which physicochemical and biological properties were regulated by adjusting the weight ratio of chitosan/gelatin/BCP-NPs.					
31393229	7	51	theme	head	1423:1426	arg1	lesions					1397:1403	large necrotic lesions	1382:1403	large necrotic lesions of rabbit femoral head	1382:1426	Finally, In vivo animal experiments revealed that new bone tissue was observed inside the scaffolds, and gradually increased with increasing months, when implanted CGB scaffolds into large necrotic lesions of rabbit femoral head.					
31393229	3	52	theme	Infrared	724:731	arg1	Spectroscopy					733:744	Infrared Spectroscopy	724:744	Infrared Spectroscopy	724:744	The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis.					
31393229	4	53	theme	swelling	790:797	arg1	ratio					799:803	equilibrium swelling ratio	778:803	equilibrium swelling ratio	778:803	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	4	54	theme	equilibrium	778:788	arg1	ratio					799:803	equilibrium swelling ratio	778:803	equilibrium swelling ratio	778:803	The porosity, equilibrium swelling ratio, and compressive strength of CGB scaffolds were mainly influenced by the BCP-NPs concentration.					
31393229	6	55	theme	stem	1179:1182	arg1	BMSCs					1191:1195	BMSCs	1191:1195	BMSCs	1191:1195	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	6	55	theme	stem	1179:1182	arg1	cells					1184:1188	mesenchymal stem cells	1167:1188	bone marrow mesenchymal stem cells (BMSCs)	1155:1196	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	1	56	theme	bone	250:253	arg1	engineering					262:272	bone tissue engineering	250:272	bone tissue engineering	250:272	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article.					
31393229	0	57	theme	nanoparticles	86:98	arg1	incorporation					42:54	incorporation	42:54	incorporation of biphasic calcium phosphate nanoparticles for bone tissue engineering	42:126	Development of chitosan/gelatin hydrogels incorporation of biphasic calcium phosphate nanoparticles for bone tissue engineering.					
31393229	3	58	theme	Microscope	678:687	arg1	images					695:700	Scanning Electron Microscope (SEM) images	660:700	Scanning Electron Microscope (SEM) images	660:700	The needle-like BCP-NPs were dispersed into composites uniformly, and physically cross-linked with chitosan and gelatin, which were identified via Scanning Electron Microscope (SEM) images and Fourier Transform Infrared Spectroscopy (FT-IR) analysis.					
31393229	5	59	theme	In	901:902	arg1	analysis					922:929	In vitro degradation analysis	901:929	In vitro degradation analysis in simulated body fluids (SBF)	901:960	In vitro degradation analysis in simulated body fluids (SBF) displayed that CGB scaffolds were degraded up to at least 30 wt% in one month.					
31393229	2	60	theme	physicochemical	397:411	arg1	properties					428:437	physicochemical and biological properties	397:437	physicochemical and biological properties	397:437	Such nanocomposite hydrogels were fabricated by using cycled freeze-thawing method, of which physicochemical and biological properties were regulated by adjusting the weight ratio of chitosan/gelatin/BCP-NPs.					
31393229	6	61	theme	good	1106:1109	arg1	cytocompatibility					1111:1127	a good cytocompatibility	1104:1127	a good cytocompatibility through	1104:1135	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	1	62	theme	tissue	255:260	arg1	engineering					262:272	bone tissue engineering	250:272	bone tissue engineering	250:272	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article.					
31393229	0	63	theme	phosphate	76:84	arg1	nanoparticles					86:98	biphasic calcium phosphate nanoparticles	59:98	biphasic calcium phosphate nanoparticles	59:98	Development of chitosan/gelatin hydrogels incorporation of biphasic calcium phosphate nanoparticles for bone tissue engineering.					
31393229	6	64	contain	had	1100:1102	arg2	cytocompatibility					1111:1127	a good cytocompatibility	1104:1127	a good cytocompatibility through	1104:1135	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	6	64	contain	had	1100:1102	arg1	scaffolds					1090:1098	the prepared scaffolds	1077:1098	the prepared scaffolds	1077:1098	Also, CCK-8 analysis confirmed that the prepared scaffolds had a good cytocompatibility through in culturing with bone marrow mesenchymal stem cells (BMSCs).					
31393229	0	65	theme	chitosan/gelatin	15:30	arg1	Development					0:10	Development	0:10	Development of chitosan/gelatin	0:30	Development of chitosan/gelatin hydrogels incorporation of biphasic calcium phosphate nanoparticles for bone tissue engineering.					
31393229	1	66	theme	calcium	186:192	arg1	BCP-NPs					219:225	BCP-NPs	219:225	BCP-NPs	219:225	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article.					
31393229	1	66	theme	calcium	186:192	arg1	nanoparticles					204:216	biphasic calcium phosphate nanoparticles	177:216	biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering	177:272	The chitosan/gelatin hydrogel incorporated with biphasic calcium phosphate nanoparticles (BCP-NPs) as scaffold (CGB) for bone tissue engineering was reported in this article.					
31393229	8	67	contain	had	1490:1492	arg1	nanocomposites					1475:1488	prepared CGB nanocomposites	1462:1488	prepared CGB nanocomposites	1462:1488	The above results suggested that prepared CGB nanocomposites had the potential to be applied in bone tissue engineering.					
31393229	8	67	contain	had	1490:1492	arg2	potential					1498:1506	the potential to be applied in bone tissue engineering	1494:1547	the potential to be applied in bone tissue engineering	1494:1547	The above results suggested that prepared CGB nanocomposites had the potential to be applied in bone tissue engineering.					
30658092	4	0	theme	cell	1228:1231	arg1	contractility					1233:1245	increased cell contractility	1218:1245	increased cell contractility	1218:1245	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	7	1	theme	injury	1826:1831	arg1	injury					1826:1831	central nervous system injury	1803:1831	central nervous system injury	1803:1831	Overall, these findings suggest that astrocyte activation not only increases cell traction, but may also contribute to hypoxia near sites of central nervous system injury.					
30658092	7	1	theme	injury	1826:1831	arg1	sites					1794:1798	sites	1794:1798	sites of central nervous system injury	1794:1831	Overall, these findings suggest that astrocyte activation not only increases cell traction, but may also contribute to hypoxia near sites of central nervous system injury.					
30658092	5	2	theme	thrombin	1263:1270	arg1	effects					1252:1258	The effects	1248:1258	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction,	1248:1340	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	2	3	theme	small	508:512	arg1	groups					514:519	small groups	508:519	small groups	508:519	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	2	4	dep	limited	473:479	arg1	whereas					562:568	whereas	562:568	whereas	562:568	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	1	5	theme	glial	181:185	arg1	formation					192:200	glial scar formation	181:200	glial scar formation	181:200	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	4	6	theme	storage	1110:1116	arg1	modulus					1118:1124	a higher shear storage modulus	1095:1124	a higher shear storage modulus of the cell/matrix composite	1095:1153	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	7	7	theme	system	1819:1824	arg1	injury					1826:1831	central nervous system injury	1803:1831	central nervous system injury	1803:1831	Overall, these findings suggest that astrocyte activation not only increases cell traction, but may also contribute to hypoxia near sites of central nervous system injury.					
30658092	3	8	theme	viscoelastic	708:719	arg1	properties					721:730	the viscoelastic properties	704:730	the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix	704:868	Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties.					
30658092	6	9	from	increase	1626:1633	arg1	expression					1650:1659	HIF-1α gene expression	1638:1659	HIF-1α gene expression	1638:1659	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	4	10	theme	shear	1104:1108	arg1	modulus					1118:1124	a higher shear storage modulus	1095:1124	a higher shear storage modulus of the cell/matrix composite	1095:1153	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	5	11	theme	blebbistatin	1276:1287	arg1	effects					1252:1258	The effects	1248:1258	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction,	1248:1340	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	2	12	theme	extracellular	538:550	arg1	matrices					552:559	extracellular matrices	538:559	extracellular matrices	538:559	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	2	13	theme	glial	675:679	arg1	scar					681:684	the glial scar	671:684	the glial scar	671:684	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	3	14	theme	surrounding	851:861	arg1	matrix					863:868	the surrounding matrix	847:868	the surrounding matrix	847:868	Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties.					
30658092	4	15	theme	collagen	999:1006	arg1	hydrogel					1008:1015	a collagen hydrogel	997:1015	a collagen hydrogel	997:1015	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	6	16	contain	have	1610:1613	arg1	cells					1568:1572	cells	1568:1572	cells in the hyaluronan-containing matrix	1568:1608	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	6	16	contain	have	1610:1613	arg2	increase					1626:1633	a ~ 3-fold increase	1615:1633	a ~ 3-fold increase in HIF-1α gene expression	1615:1659	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	6	17	theme	gene	1645:1648	arg1	expression					1650:1659	HIF-1α gene expression	1638:1659	HIF-1α gene expression	1638:1659	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	1	18	theme	scar	187:190	arg1	formation					192:200	glial scar formation	181:200	glial scar formation	181:200	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	4	19	theme	filament	1058:1065	arg1	GFAP					1075:1078	the intermediate filament protein GFAP	1041:1078	the intermediate filament protein GFAP	1041:1078	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	3	20	theme	cell-seeded	735:745	arg1	hydrogels					747:755	cell-seeded hydrogels	735:755	cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix	735:868	Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties.					
30658092	6	21	theme	scaffold	1518:1525	arg1	center					1504:1509	the center	1500:1509	the center of the scaffold	1500:1525	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	4	22	theme	GFAP	1075:1078	arg1	expression					1027:1036	expression	1027:1036	expression of the intermediate filament protein GFAP	1027:1078	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	6	23	theme	HIF-1α	1638:1643	arg1	expression					1650:1659	HIF-1α gene expression	1638:1659	HIF-1α gene expression	1638:1659	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	6	24	theme	cell	1460:1463	arg1	traction					1465:1472	increased cell traction	1450:1472	increased cell traction	1450:1472	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	6	25	theme	level	1540:1544	arg1	analysis					1546:1553	message level analysis	1532:1553	message level analysis	1532:1553	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	3	26	theme	hydrogels	747:755	arg1	properties					721:730	the viscoelastic properties	704:730	the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix	704:868	Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties.					
30658092	2	27	theme	functional	643:652	arg1	syncytium					654:662	a functional syncytium	641:662	a functional syncytium	641:662	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	4	28	theme	astrocyte	1193:1201	arg1	activation					1203:1212	astrocyte activation	1193:1212	astrocyte activation	1193:1212	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	0	29	theme	Cell-matrix	0:10	arg1	tension					12:18	Cell-matrix tension	0:18	Cell-matrix tension	0:18	Cell-matrix tension contributes to hypoxia in astrocyte-seeded viscoelastic hydrogels composed of collagen and hyaluronan.					
30658092	1	30	theme	astrocyte	290:298	arg1	contractility					300:312	astrocyte contractility	290:312	astrocyte contractility	290:312	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	5	31	theme	hydrogel	1387:1394	arg1	properties					1407:1416	the hydrogel mechanical properties	1383:1416	the hydrogel mechanical properties	1383:1416	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	1	32	theme	central	212:218	arg1	system					228:233	central nervous system	212:233	central nervous system injury	212:240	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	3	33	theme	collective	783:792	arg1	contractility					794:806	the collective contractility	779:806	the collective contractility of astrocytes	779:820	Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties.					
30658092	4	34	theme	CNS	960:962	arg1	hyaluronan					981:990	the CNS matrix component hyaluronan	956:990	the CNS matrix component hyaluronan	956:990	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	6	35	theme	increased	1450:1458	arg1	traction					1465:1472	increased cell traction	1450:1472	increased cell traction	1450:1472	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	6	36	theme	cell	1486:1489	arg1	death					1491:1495	cell death	1486:1495	cell death	1486:1495	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	4	37	theme	composite	1145:1153	arg1	modulus					1118:1124	a higher shear storage modulus	1095:1124	a higher shear storage modulus of the cell/matrix composite	1095:1153	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	1	38	theme	nervous	220:226	arg1	system					228:233	central nervous system	212:233	central nervous system injury	212:240	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	2	39	theme	three-dimensional	441:457	arg1	scaffolds					459:467	three-dimensional scaffolds	441:467	three-dimensional scaffolds	441:467	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	7	40	theme	cell	1739:1742	arg1	traction					1744:1751	cell traction	1739:1751	cell traction	1739:1751	Overall, these findings suggest that astrocyte activation not only increases cell traction, but may also contribute to hypoxia near sites of central nervous system injury.					
30658092	2	41	theme	gap	570:572	arg1	junctions					574:582	gap junctions	570:582	gap junctions	570:582	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	1	42	theme	Astrocyte	123:131	arg1	activation					133:142	Astrocyte activation	123:142	Astrocyte activation	123:142	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	1	43	theme	system	228:233	arg1	injury					235:240	central nervous system injury	212:240	central nervous system injury	212:240	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	4	44	theme	intermediate	1045:1056	arg1	GFAP					1075:1078	the intermediate filament protein GFAP	1041:1078	the intermediate filament protein GFAP	1041:1078	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	1	45	theme	matrix	318:323	arg1	reorganization					325:338	matrix reorganization	318:338	matrix reorganization	318:338	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	4	46	theme	component	971:979	arg1	hyaluronan					981:990	the CNS matrix component hyaluronan	956:990	the CNS matrix component hyaluronan	956:990	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	7	47	theme	central	1803:1809	arg1	system					1819:1824	central nervous system	1803:1824	central nervous system injury	1803:1831	Overall, these findings suggest that astrocyte activation not only increases cell traction, but may also contribute to hypoxia near sites of central nervous system injury.					
30658092	2	48	theme	Current	388:394	arg1	means					396:400	Current means	388:400	Current means to measure cell traction forces within three-dimensional scaffolds	388:467	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	2	49	theme	traction	418:425	arg1	forces					427:432	cell traction forces	413:432	cell traction forces	413:432	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	5	50	theme	cell-matrix	1354:1364	arg1	tension					1366:1372	cell-matrix tension	1354:1372	cell-matrix tension	1354:1372	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	3	51	theme	astrocytes	811:820	arg1	contractility					794:806	the collective contractility	779:806	the collective contractility of astrocytes	779:820	Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties.					
30658092	4	52	theme	matrix	964:969	arg1	hyaluronan					981:990	the CNS matrix component hyaluronan	956:990	the CNS matrix component hyaluronan	956:990	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	2	53	theme	cell	413:416	arg1	forces					427:432	cell traction forces	413:432	cell traction forces	413:432	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	0	54	theme	viscoelastic	63:74	arg1	hydrogels					76:84	astrocyte-seeded viscoelastic hydrogels	46:84	astrocyte-seeded viscoelastic hydrogels composed of collagen and hyaluronan	46:120	Cell-matrix tension contributes to hypoxia in astrocyte-seeded viscoelastic hydrogels composed of collagen and hyaluronan.					
30658092	3	55	dep	hydrogels	747:755	arg1	yield					760:764	yield	760:764	to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix	757:868	Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties.					
30658092	6	56	theme	message	1532:1538	arg1	analysis					1546:1553	message level analysis	1532:1553	message level analysis	1532:1553	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	0	57	theme	astrocyte-seeded	46:61	arg1	hydrogels					76:84	astrocyte-seeded viscoelastic hydrogels	46:84	astrocyte-seeded viscoelastic hydrogels composed of collagen and hyaluronan	46:120	Cell-matrix tension contributes to hypoxia in astrocyte-seeded viscoelastic hydrogels composed of collagen and hyaluronan.					
30658092	2	58	theme	cell-cell	594:602	arg1	adhesions					604:612	other cell-cell adhesions	588:612	other cell-cell adhesions	588:612	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	3	59	theme	bulk	885:888	arg1	properties					901:910	its bulk mechanical properties	881:910	its bulk mechanical properties	881:910	Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties.					
30658092	1	60	theme	central	347:353	arg1	CNS					371:373	CNS	371:373	CNS	371:373	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	1	60	theme	central	347:353	arg1	system					363:368	the central nervous system	343:368	the central nervous system (CNS)	343:374	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	2	61	theme	other	588:592	arg1	adhesions					604:612	other cell-cell adhesions	588:612	other cell-cell adhesions	588:612	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	6	62	theme	~ 3-fold	1617:1624	arg1	increase					1626:1633	a ~ 3-fold increase	1615:1633	a ~ 3-fold increase in HIF-1α gene expression	1615:1659	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	2	63	dep	means	396:400	arg1	measure					405:411	measure	405:411	to measure cell traction forces within three-dimensional scaffolds	402:467	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	6	64	theme	Viability	1419:1427	arg1	assays					1429:1434	Viability assays	1419:1434	Viability assays	1419:1434	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	3	65	theme	mechanical	890:899	arg1	properties					901:910	its bulk mechanical properties	881:910	its bulk mechanical properties	881:910	Here, we measure the viscoelastic properties of cell-seeded hydrogels to yield insight into the collective contractility of astrocytes as they exert tension on the surrounding matrix and change its bulk mechanical properties.					
30658092	5	66	theme	known	1290:1294	arg1	mediators					1296:1304	known mediators	1290:1304	known mediators of actomyosin-mediated contraction	1290:1339	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	5	66	theme	known	1290:1294	arg1	thrombin					1263:1270	thrombin	1263:1270	thrombin	1263:1270	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	4	67	theme	increased	1218:1226	arg1	contractility					1233:1245	increased cell contractility	1218:1245	increased cell contractility	1218:1245	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	5	68	theme	mechanical	1396:1405	arg1	properties					1407:1416	the hydrogel mechanical properties	1383:1416	the hydrogel mechanical properties	1383:1416	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	4	69	theme	cell/matrix	1133:1143	arg1	composite					1145:1153	the cell/matrix composite	1129:1153	the cell/matrix composite	1129:1153	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	7	70	theme	astrocyte	1699:1707	arg1	activation					1709:1718	astrocyte activation	1699:1718	astrocyte activation	1699:1718	Overall, these findings suggest that astrocyte activation not only increases cell traction, but may also contribute to hypoxia near sites of central nervous system injury.					
30658092	2	71	theme	cells	524:528	arg1	individual					494:503	individual	494:503	individual	494:503	Current means to measure cell traction forces within three-dimensional scaffolds are limited to analyzing individual or small groups of cells, within extracellular matrices, whereas gap junctions and other cell-cell adhesions connect astrocytes to form a functional syncytium within the glial scar.					
30658092	7	72	theme	nervous	1811:1817	arg1	system					1819:1824	central nervous system	1803:1824	central nervous system injury	1803:1831	Overall, these findings suggest that astrocyte activation not only increases cell traction, but may also contribute to hypoxia near sites of central nervous system injury.					
30658092	6	73	from	cells	1568:1572	arg1	matrix					1603:1608	the hyaluronan-containing matrix	1577:1608	the hyaluronan-containing matrix	1577:1608	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	5	74	theme	contraction	1329:1339	arg1	mediators					1296:1304	known mediators	1290:1304	known mediators of actomyosin-mediated contraction	1290:1339	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	5	74	theme	contraction	1329:1339	arg1	thrombin					1263:1270	thrombin	1263:1270	thrombin	1263:1270	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	1	75	theme	wound	159:163	arg1	contraction					165:175	wound contraction	159:175	wound contraction	159:175	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	6	76	theme	hyaluronan-containing	1581:1601	arg1	matrix					1603:1608	the hyaluronan-containing matrix	1577:1608	the hyaluronan-containing matrix	1577:1608	Viability assays indicate that increased cell traction exacerbates cell death at the center of the scaffold, and message level analysis reveals that cells in the hyaluronan-containing matrix have a ~ 3-fold increase in HIF-1α gene expression.					
30658092	4	77	theme	protein	1067:1073	arg1	GFAP					1075:1078	the intermediate filament protein GFAP	1041:1078	the intermediate filament protein GFAP	1041:1078	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	1	78	theme	nervous	355:361	arg1	CNS					371:373	CNS	371:373	CNS	371:373	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	1	78	theme	nervous	355:361	arg1	system					363:368	the central nervous system	343:368	the central nervous system (CNS)	343:374	Astrocyte activation is crucial for wound contraction and glial scar formation following central nervous system injury, but the mechanism by which activation leads to astrocyte contractility and matrix reorganization in the central nervous system (CNS) is unknown.					
30658092	5	79	theme	actomyosin-mediated	1309:1327	arg1	contraction					1329:1339	actomyosin-mediated contraction	1309:1339	actomyosin-mediated contraction	1309:1339	The effects of thrombin and blebbistatin, known mediators of actomyosin-mediated contraction, verify that cell-matrix tension dictates the hydrogel mechanical properties.					
30658092	4	80	theme	higher	1097:1102	arg1	modulus					1118:1124	a higher shear storage modulus	1095:1124	a higher shear storage modulus of the cell/matrix composite	1095:1153	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
30658092	4	81	theme	hyaluronan	981:990	arg1	incorporation					939:951	incorporation	939:951	incorporation of the CNS matrix component hyaluronan into a collagen hydrogel	939:1015	Our results indicate that incorporation of the CNS matrix component hyaluronan into a collagen hydrogel increases expression of the intermediate filament protein GFAP and results in a higher shear storage modulus of the cell/matrix composite, establishing the correlation between astrocyte activation and increased cell contractility.					
31819069	9	0	theme	main	869:872	arg1	Firmicutes					881:890	Firmicutes	881:890	Firmicutes	881:890	Irrespective of diet, the bacterial profile was dominated by two main phyla, Firmicutes, closely followed by Bacteroidetes.					
31819069	9	0	theme	main	869:872	arg1	phyla					874:878	two main phyla	865:878	two main phyla	865:878	Irrespective of diet, the bacterial profile was dominated by two main phyla, Firmicutes, closely followed by Bacteroidetes.					
31819069	14	1	theme	gut	1378:1380	arg1	microbiota					1382:1391	gut microbiota	1378:1391	gut microbiota	1378:1391	Dietary induced alterations to gut microbiota play a role in affecting the behaviour of the host.					
31819069	10	2	dep	showed	1018:1023	arg1	affected					1030:1037	affected	1030:1037	showed diet affected faecal microbial community structure	1018:1074	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	8	3	theme	HF	795:796	arg1	diet					798:801	the HF diet	791:801	the HF diet	791:801	Results showed that ponies were more reactive and less settled when being fed the HS diet compared to the HF diet.					
31819069	7	4	used	used	612:615	arg2	design					601:606	A cross-over design	588:606	A cross-over design	588:606	A cross-over design was used with experimental groups fed high-starch (HS) or high-fibre (HF) diets.					
31819069	6	5	theme	faecal	536:541	arg1	microbiota					543:552	faecal microbiota	536:552	faecal microbiota	536:552	This study compares the faecal microbiota and behaviour of 10 naïve ponies.					
31819069	1	6	theme	several	160:166	arg1	species					168:174	several species	160:174	several species	160:174	Gut microbiota have been associated with health, disease and behaviour in several species and are an important link in gut-brain axis communication.					
31819069	10	7	theme	sequencing	1002:1011	arg1	data					1013:1016	gene sequencing data	997:1016	16S rRNA gene sequencing data	988:1016	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	7	8	theme	cross-over	590:599	arg1	design					601:606	A cross-over design	588:606	A cross-over design	588:606	A cross-over design was used with experimental groups fed high-starch (HS) or high-fibre (HF) diets.					
31819069	14	9	theme	host	1439:1442	arg1	behaviour					1422:1430	the behaviour	1418:1430	the behaviour of the host	1418:1442	Dietary induced alterations to gut microbiota play a role in affecting the behaviour of the host.					
31819069	8	10	theme	HS	771:772	arg1	diet					774:777	the HS diet	767:777	the HS diet	767:777	Results showed that ponies were more reactive and less settled when being fed the HS diet compared to the HF diet.					
31819069	3	11	theme	hindgut	344:350	arg1	microbiota					352:361	the hindgut microbiota	340:361	the hindgut microbiota	340:361	In horses, high-starch diets alter the hindgut microbiota.					
31819069	10	12	theme	community	1056:1064	arg1	structure					1066:1074	faecal microbial community structure	1039:1074	faecal microbial community structure	1039:1074	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	10	13	theme	rRNA	992:995	arg1	analysis					976:983	multivariate analysis	963:983	multivariate analysis of 16S rRNA gene sequencing data	963:1016	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	7	14	dep	high-fibre	666:675	arg1	HF					678:679	HF	678:679	HF	678:679	A cross-over design was used with experimental groups fed high-starch (HS) or high-fibre (HF) diets.					
31819069	14	15	theme	Dietary	1347:1353	arg1	alterations					1363:1373	Dietary induced alterations	1347:1373	Dietary induced alterations to gut microbiota	1347:1391	Dietary induced alterations to gut microbiota play a role in affecting the behaviour of the host.					
31819069	7	16	theme	experimental	622:633	arg1	groups					635:640	experimental groups	622:640	experimental groups fed high-starch (HS) or high-fibre (HF) diets	622:686	A cross-over design was used with experimental groups fed high-starch (HS) or high-fibre (HF) diets.					
31819069	6	17	dep	microbiota	543:552	arg1	the					532:534	the	532:534	the	532:534	This study compares the faecal microbiota and behaviour of 10 naïve ponies.					
31819069	1	18	from	behaviour	147:155	arg1	species					168:174	several species	160:174	several species	160:174	Gut microbiota have been associated with health, disease and behaviour in several species and are an important link in gut-brain axis communication.					
31819069	13	19	theme	faecal	1293:1298	arg1	composition					1320:1330	faecal microbial community composition	1293:1330	faecal microbial community composition	1293:1330	Results demonstrate a clear link between diet, faecal microbial community composition and behaviour.					
31819069	13	20	theme	microbial	1300:1308	arg1	composition					1320:1330	faecal microbial community composition	1293:1330	faecal microbial community composition	1293:1330	Results demonstrate a clear link between diet, faecal microbial community composition and behaviour.					
31819069	10	21	theme	faecal	1039:1044	arg1	structure					1066:1074	faecal microbial community structure	1039:1074	faecal microbial community structure	1039:1074	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	1	22	theme	Gut	86:88	arg1	microbiota					90:99	Gut microbiota	86:99	Gut microbiota	86:99	Gut microbiota have been associated with health, disease and behaviour in several species and are an important link in gut-brain axis communication.					
31819069	11	23	theme	OTUs	1097:1100	arg1	abundance					1081:1089	The abundance	1077:1089	The abundance of 85 OTUs	1077:1100	The abundance of 85 OTUs differed significantly related to diet.					
31819069	1	24	from	disease	135:141	arg1	species					168:174	several species	160:174	several species	160:174	Gut microbiota have been associated with health, disease and behaviour in several species and are an important link in gut-brain axis communication.					
31819069	10	25	theme	microbial	1046:1054	arg1	structure					1066:1074	faecal microbial community structure	1039:1074	faecal microbial community structure	1039:1074	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	2	26	theme	microbiota	293:302	arg1	composition					274:284	the composition	270:284	the composition of gut microbiota	270:302	Diet plays a key role in affecting the composition of gut microbiota.					
31819069	13	27	theme	clear	1268:1272	arg1	link					1274:1277	a clear link	1266:1277	a clear link between diet, faecal microbial community composition and behaviour	1266:1344	Results demonstrate a clear link between diet, faecal microbial community composition and behaviour.					
31819069	0	28	theme	equine	24:29	arg1	microbiota					38:47	equine faecal microbiota	24:47	equine faecal microbiota	24:47	High-starch diets alter equine faecal microbiota and increase behavioural reactivity.					
31819069	4	29	theme	High-starch	364:374	arg1	diets					376:380	High-starch diets	364:380	High-starch diets	364:380	High-starch diets are also associated with increased behavioural reactivity in horses.					
31819069	2	30	theme	gut	289:291	arg1	microbiota					293:302	gut microbiota	289:302	gut microbiota	289:302	Diet plays a key role in affecting the composition of gut microbiota.					
31819069	1	31	theme	important	187:195	arg1	link					197:200	an important link	184:200	an important link in gut-brain axis communication	184:232	Gut microbiota have been associated with health, disease and behaviour in several species and are an important link in gut-brain axis communication.					
31819069	5	32	from	changes	457:463	arg1	behaviour					483:491	behaviour	483:491	behaviour	483:491	These changes in microbiota and behaviour may be associated.					
31819069	5	32	from	changes	457:463	arg1	microbiota					468:477	microbiota	468:477	microbiota	468:477	These changes in microbiota and behaviour may be associated.					
31819069	7	33	dep	high-starch	646:656	arg1	HS					659:660	HS	659:660	HS	659:660	A cross-over design was used with experimental groups fed high-starch (HS) or high-fibre (HF) diets.					
31819069	9	34	theme	diet	820:823	arg1	Irrespective					804:815	Irrespective	804:815	Irrespective	804:815	Irrespective of diet, the bacterial profile was dominated by two main phyla, Firmicutes, closely followed by Bacteroidetes.					
31819069	12	35	theme	induced	1190:1196	arg1	alterations					1198:1208	dietary induced alterations	1182:1208	dietary induced alterations to faecal microbiota and behaviour	1182:1243	Correlative relationships exist between dietary induced alterations to faecal microbiota and behaviour.					
31819069	6	36	theme	ponies	580:585	arg1	microbiota					543:552	faecal microbiota	536:552	faecal microbiota	536:552	This study compares the faecal microbiota and behaviour of 10 naïve ponies.					
31819069	6	36	theme	ponies	580:585	arg1	behaviour					558:566	behaviour	558:566	behaviour	558:566	This study compares the faecal microbiota and behaviour of 10 naïve ponies.					
31819069	10	37	theme	multivariate	963:974	arg1	analysis					976:983	multivariate analysis	963:983	multivariate analysis of 16S rRNA gene sequencing data	963:1016	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	0	38	theme	faecal	31:36	arg1	microbiota					38:47	equine faecal microbiota	24:47	equine faecal microbiota	24:47	High-starch diets alter equine faecal microbiota and increase behavioural reactivity.					
31819069	6	39	theme	naïve	574:578	arg1	ponies					580:585	10 naïve ponies	571:585	10 naïve ponies	571:585	This study compares the faecal microbiota and behaviour of 10 naïve ponies.					
31819069	1	40	theme	gut-brain	205:213	arg1	communication					220:232	gut-brain axis communication	205:232	gut-brain axis communication	205:232	Gut microbiota have been associated with health, disease and behaviour in several species and are an important link in gut-brain axis communication.					
31819069	9	41	theme	bacterial	830:838	arg1	profile					840:846	the bacterial profile	826:846	the bacterial profile	826:846	Irrespective of diet, the bacterial profile was dominated by two main phyla, Firmicutes, closely followed by Bacteroidetes.					
31819069	0	42	theme	behavioural	62:72	arg1	reactivity					74:83	behavioural reactivity	62:83	behavioural reactivity	62:83	High-starch diets alter equine faecal microbiota and increase behavioural reactivity.					
31819069	12	43	theme	faecal	1213:1218	arg1	microbiota					1220:1229	faecal microbiota	1213:1229	faecal microbiota	1213:1229	Correlative relationships exist between dietary induced alterations to faecal microbiota and behaviour.					
31819069	4	44	from	reactivity	429:438	arg1	horses					443:448	horses	443:448	horses	443:448	High-starch diets are also associated with increased behavioural reactivity in horses.					
31819069	4	45	theme	behavioural	417:427	arg1	reactivity					429:438	increased behavioural reactivity	407:438	increased behavioural reactivity in horses	407:448	High-starch diets are also associated with increased behavioural reactivity in horses.					
31819069	7	46	theme	high-fibre	666:675	arg1	diets					682:686	high-starch (HS) or high-fibre (HF) diets	646:686	high-starch (HS) or high-fibre (HF) diets	646:686	A cross-over design was used with experimental groups fed high-starch (HS) or high-fibre (HF) diets.					
31819069	10	47	theme	taxonomic	946:954	arg1	levels					956:961	lower taxonomic levels	940:961	lower taxonomic levels	940:961	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	1	48	from	health	127:132	arg1	species					168:174	several species	160:174	several species	160:174	Gut microbiota have been associated with health, disease and behaviour in several species and are an important link in gut-brain axis communication.					
31819069	4	49	theme	increased	407:415	arg1	reactivity					429:438	increased behavioural reactivity	407:438	increased behavioural reactivity in horses	407:448	High-starch diets are also associated with increased behavioural reactivity in horses.					
31819069	10	50	theme	lower	940:944	arg1	levels					956:961	lower taxonomic levels	940:961	lower taxonomic levels	940:961	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	1	51	from	link	197:200	arg1	communication					220:232	gut-brain axis communication	205:232	gut-brain axis communication	205:232	Gut microbiota have been associated with health, disease and behaviour in several species and are an important link in gut-brain axis communication.					
31819069	14	52	theme	induced	1355:1361	arg1	alterations					1363:1373	Dietary induced alterations	1347:1373	Dietary induced alterations to gut microbiota	1347:1391	Dietary induced alterations to gut microbiota play a role in affecting the behaviour of the host.					
31819069	1	53	theme	axis	215:218	arg1	communication					220:232	gut-brain axis communication	205:232	gut-brain axis communication	205:232	Gut microbiota have been associated with health, disease and behaviour in several species and are an important link in gut-brain axis communication.					
31819069	10	54	theme	16S	988:990	arg1	rRNA					992:995	16S rRNA gene sequencing data	988:1016	16S rRNA gene sequencing data	988:1016	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	2	55	theme	key	248:250	arg1	role					252:255	a key role	246:255	a key role	246:255	Diet plays a key role in affecting the composition of gut microbiota.					
31819069	12	56	theme	Correlative	1142:1152	arg1	relationships					1154:1166	Correlative relationships	1142:1166	Correlative relationships	1142:1166	Correlative relationships exist between dietary induced alterations to faecal microbiota and behaviour.					
31819069	0	57	dep	diets	12:16	arg1	alter					18:22	alter	18:22	alter equine faecal microbiota	18:47	High-starch diets alter equine faecal microbiota and increase behavioural reactivity.					
31819069	0	57	dep	diets	12:16	arg1	increase					53:60	increase	53:60	increase behavioural reactivity	53:83	High-starch diets alter equine faecal microbiota and increase behavioural reactivity.					
31819069	12	58	theme	dietary	1182:1188	arg1	alterations					1198:1208	dietary induced alterations	1182:1208	dietary induced alterations to faecal microbiota and behaviour	1182:1243	Correlative relationships exist between dietary induced alterations to faecal microbiota and behaviour.					
31819069	3	59	theme	high-starch	316:326	arg1	diets					328:332	high-starch diets	316:332	high-starch diets	316:332	In horses, high-starch diets alter the hindgut microbiota.					
31819069	7	60	theme	high-starch	646:656	arg1	diets					682:686	high-starch (HS) or high-fibre (HF) diets	646:686	high-starch (HS) or high-fibre (HF) diets	646:686	A cross-over design was used with experimental groups fed high-starch (HS) or high-fibre (HF) diets.					
31819069	10	61	dep	rRNA	992:995	arg1	data					1013:1016	gene sequencing data	997:1016	16S rRNA gene sequencing data	988:1016	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	10	62	theme	gene	997:1000	arg1	data					1013:1016	gene sequencing data	997:1016	16S rRNA gene sequencing data	988:1016	However, at lower taxonomic levels multivariate analysis of 16S rRNA gene sequencing data showed diet affected faecal microbial community structure.					
31819069	13	63	theme	community	1310:1318	arg1	composition					1320:1330	faecal microbial community composition	1293:1330	faecal microbial community composition	1293:1330	Results demonstrate a clear link between diet, faecal microbial community composition and behaviour.					
31819069	9	64	dep	dominated	852:860	arg1	followed					901:908	followed	901:908	followed by Bacteroidetes	901:925	Irrespective of diet, the bacterial profile was dominated by two main phyla, Firmicutes, closely followed by Bacteroidetes.					
31212920	4	0	theme	mass	920:923	arg1	spectrometry					925:936	mass spectrometry	920:936	mass spectrometry	920:936	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	1	1	theme	brain	340:344	arg1	development					346:356	brain development	340:356	brain development in infants	340:367	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	5	2	theme	allergic	1158:1165	arg1	disease					1167:1173	maternal allergic disease	1149:1173	maternal allergic disease	1149:1173	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	6	3	theme	novel	1340:1344	arg1	associations					1346:1357	novel associations	1340:1357	novel associations of maternal allergic disease and infant's sex with HMOs concentrations	1340:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	5	4	theme	HMOs	1035:1038	arg1	concentrations					1040:1053	HMOs concentrations	1035:1053	HMOs concentrations	1035:1053	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	4	5	theme	cross-sectional	657:671	arg1	study					688:692	this cross-sectional, observational study	652:692	this cross-sectional, observational study	652:692	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	6	6	theme	HMOs	1410:1413	arg1	concentrations					1415:1428	HMOs concentrations	1410:1428	HMOs concentrations	1410:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	1	7	theme	pathogen	264:271	arg1	binding					273:279	pathogen binding	264:279	pathogen binding	264:279	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	6	8	theme	Brazilian	1320:1328	arg1	women					1330:1334	breastfeeding Brazilian women	1306:1334	breastfeeding Brazilian women	1306:1334	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	5	9	theme	weight	1218:1223	arg1	gain					1225:1228	weight gain	1218:1228	weight gain	1218:1228	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	7	10	theme	infant	1515:1520	arg1	growth					1522:1527	infant growth	1515:1527	infant growth	1515:1527	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	4	11	theme	representative	864:877	arg1	HMOs					879:882	16 representative HMOs	861:882	16 representative HMOs	861:882	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	4	12	dep	cross-sectional	657:671	arg1	observational					674:686	observational	674:686	observational	674:686	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	5	13	theme	time	1176:1179	arg1	postpartum					1181:1190	time postpartum	1176:1190	time postpartum	1176:1190	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	4	14	theme	liquid	887:892	arg1	chromatography					894:907	liquid chromatography	887:907	liquid chromatography coupled to mass spectrometry	887:936	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	2	15	theme	HMOs	429:432	arg1	concentrations					434:447	HMOs concentrations	429:447	HMOs concentrations	429:447	HMOs composition is very individualized and differences in HMOs concentrations may affect the infant's health.					
31212920	6	16	from	women	1330:1334	arg1	concentrations					1286:1299	HMOs concentrations	1281:1299	HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations	1281:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	1	17	theme	multifunctional	170:184	arg1	oligosaccharides					142:157	Human milk oligosaccharides	131:157	Human milk oligosaccharides (HMOs)	131:164	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	17	theme	multifunctional	170:184	arg1	carbohydrates					186:198	multifunctional carbohydrates	170:198	multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants	170:367	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	0	18	theme	Lewis	113:117	arg1	Phenotypes					119:128	Lewis Phenotypes	113:128	Lewis Phenotypes	113:128	Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes.					
31212920	5	19	theme	high	1067:1070	arg1	variability					1072:1082	a high variability	1065:1082	a high variability	1065:1082	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	4	20	dep	collected	802:810	arg1	analyzed					848:855	analyzed	848:855	analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry	848:936	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	3	21	theme	Secretor	544:551	arg1	genes					573:577	Secretor (Se) and Lewis (Le) genes	544:577	Secretor (Se) and Lewis (Le) genes	544:577	HMOs variability can be partially explained by the activity of Secretor (Se) and Lewis (Le) genes in the mother, but non-genetic maternal factors may also be involved.					
31212920	6	22	theme	disease	1380:1386	arg1	women					1330:1334	breastfeeding Brazilian women	1306:1334	breastfeeding Brazilian women	1306:1334	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	6	22	theme	disease	1380:1386	arg1	associations					1346:1357	novel associations	1340:1357	novel associations of maternal allergic disease and infant's sex with HMOs concentrations	1340:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	7	23	theme	HMOs	1568:1571	arg1	concentrations					1541:1554	higher concentrations	1534:1554	higher concentrations of specific HMOs	1534:1571	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	7	24	theme	weight	1603:1608	arg1	gain					1610:1613	excessive weight gain	1593:1613	excessive weight gain	1593:1613	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	4	25	with	associations	942:953	arg1	concentrations					1001:1014	HMOs concentrations	996:1014	HMOs concentrations	996:1014	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	3	26	theme	Lewis	562:566	arg1	genes					573:577	Secretor (Se) and Lewis (Le) genes	544:577	Secretor (Se) and Lewis (Le) genes	544:577	HMOs variability can be partially explained by the activity of Secretor (Se) and Lewis (Le) genes in the mother, but non-genetic maternal factors may also be involved.					
31212920	4	27	theme	human	705:709	arg1	samples					716:722	78 single human milk samples	695:722	78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days)	695:795	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	6	28	theme	maternal	1362:1369	arg1	disease					1380:1386	maternal allergic disease	1362:1386	maternal allergic disease	1362:1386	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	0	29	theme	Maternal	0:7	arg1	Factors					20:26	Maternal and Infant Factors	0:26	Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations	0:85	Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes.					
31212920	6	30	from	data	1273:1276	arg1	concentrations					1286:1299	HMOs concentrations	1281:1299	HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations	1281:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	4	31	theme	maternal	963:970	arg1	factors					983:989	maternal and infant factors	963:989	maternal and infant factors	963:989	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	1	32	theme	present	210:216	arg1	oligosaccharides					142:157	Human milk oligosaccharides	131:157	Human milk oligosaccharides (HMOs)	131:164	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	32	theme	present	210:216	arg1	carbohydrates					186:198	multifunctional carbohydrates	170:198	multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants	170:367	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	4	33	dep	IQR	780:782	arg1	days					791:794	25-46 days	785:794	IQR: 25-46 days	780:794	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	4	34	theme	single	698:703	arg1	samples					716:722	78 single human milk samples	695:722	78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days)	695:795	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	6	35	theme	HMOs	1281:1284	arg1	concentrations					1286:1299	HMOs concentrations	1281:1299	HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations	1281:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	3	36	from	activity	532:539	arg1	mother					586:591	the mother	582:591	the mother	582:591	HMOs variability can be partially explained by the activity of Secretor (Se) and Lewis (Le) genes in the mother, but non-genetic maternal factors may also be involved.					
31212920	6	37	from	associations	1346:1357	arg1	concentrations					1286:1299	HMOs concentrations	1281:1299	HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations	1281:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	1	38	from	present	210:216	arg1	milk					227:230	human milk	221:230	human milk	221:230	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	7	39	theme	excessive	1593:1601	arg1	gain					1610:1613	excessive weight gain	1593:1613	excessive weight gain	1593:1613	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	4	40	theme	milk	711:714	arg1	samples					716:722	78 single human milk samples	695:722	78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days)	695:795	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	3	41	theme	non-genetic	598:608	arg1	factors					619:625	non-genetic maternal factors	598:625	non-genetic maternal factors	598:625	HMOs variability can be partially explained by the activity of Secretor (Se) and Lewis (Le) genes in the mother, but non-genetic maternal factors may also be involved.					
31212920	1	42	theme	human	221:225	arg1	milk					227:230	human milk	221:230	human milk	221:230	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	43	from	development	346:356	arg1	infants					361:367	infants	361:367	infants	361:367	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	5	44	theme	maternal	1149:1156	arg1	disease					1167:1173	maternal allergic disease	1149:1173	maternal allergic disease	1149:1173	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	3	45	theme	genes	573:577	arg1	activity					532:539	the activity	528:539	the activity of Secretor (Se) and Lewis (Le) genes in the mother	528:591	HMOs variability can be partially explained by the activity of Secretor (Se) and Lewis (Le) genes in the mother, but non-genetic maternal factors may also be involved.					
31212920	7	46	from	Differences	1431:1441	arg1	composition					1451:1461	HMOs composition	1446:1461	HMOs composition attributed to maternal SeLe phenotype	1446:1499	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	6	47	theme	allergic	1371:1378	arg1	disease					1380:1386	maternal allergic disease	1362:1386	maternal allergic disease	1362:1386	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	1	48	theme	immune	310:315	arg1	system					317:322	the immune system	306:322	the immune system	306:322	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	6	49	theme	unprecedented	1259:1271	arg1	data					1273:1276	unprecedented data	1259:1276	unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations	1259:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	1	50	attach	present	210:216	arg2	oligosaccharides					142:157	Human milk oligosaccharides	131:157	Human milk oligosaccharides (HMOs)	131:164	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	50	attach	present	210:216	arg1	milk					227:230	human milk	221:230	human milk	221:230	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	50	attach	present	210:216	arg2	carbohydrates					186:198	multifunctional carbohydrates	170:198	multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants	170:367	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	51	from	milk	227:230	arg1	present					210:216	present	210:216	present	210:216	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	4	52	dep	median	763:768	arg1	IQR					780:782	IQR	780:782	IQR: 25-46 days	780:794	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	4	52	dep	median	763:768	arg1	days					774:777	32 days	771:777	32 days	771:777	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	0	53	theme	Milk	50:53	arg1	Concentrations					72:85	Human Milk Oligosaccharides Concentrations	44:85	Human Milk Oligosaccharides Concentrations	44:85	Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes.					
31212920	5	54	theme	same	1107:1110	arg1	phenotype					1117:1125	the same SeLe phenotype	1103:1125	the same SeLe phenotype	1103:1125	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	4	55	theme	breastfeeding	817:829	arg1	women					841:845	breastfeeding Brazilian women	817:845	breastfeeding Brazilian women	817:845	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	3	56	theme	maternal	610:617	arg1	factors					619:625	non-genetic maternal factors	598:625	non-genetic maternal factors	598:625	HMOs variability can be partially explained by the activity of Secretor (Se) and Lewis (Le) genes in the mother, but non-genetic maternal factors may also be involved.					
31212920	6	57	with	women	1330:1334	arg1	concentrations					1415:1428	HMOs concentrations	1410:1428	HMOs concentrations	1410:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	3	58	theme	HMOs	481:484	arg1	variability					486:496	HMOs variability	481:496	HMOs variability	481:496	HMOs variability can be partially explained by the activity of Secretor (Se) and Lewis (Le) genes in the mother, but non-genetic maternal factors may also be involved.					
31212920	0	59	theme	Human	44:48	arg1	Concentrations					72:85	Human Milk Oligosaccharides Concentrations	44:85	Human Milk Oligosaccharides Concentrations	44:85	Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes.					
31212920	5	60	theme	SeLe	1112:1115	arg1	phenotype					1117:1125	the same SeLe phenotype	1103:1125	the same SeLe phenotype	1103:1125	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	7	61	theme	maternal	1477:1484	arg1	phenotype					1491:1499	maternal SeLe phenotype	1477:1499	maternal SeLe phenotype	1477:1499	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	7	62	theme	SeLe	1486:1489	arg1	phenotype					1491:1499	maternal SeLe phenotype	1477:1499	maternal SeLe phenotype	1477:1499	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	6	63	with	associations	1346:1357	arg1	concentrations					1415:1428	HMOs concentrations	1410:1428	HMOs concentrations	1410:1428	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
31212920	1	64	theme	Human	131:135	arg1	HMOs					160:163	HMOs	160:163	HMOs	160:163	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	64	theme	Human	131:135	arg1	oligosaccharides					142:157	Human milk oligosaccharides	131:157	Human milk oligosaccharides (HMOs)	131:164	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	64	theme	Human	131:135	arg1	carbohydrates					186:198	multifunctional carbohydrates	170:198	multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants	170:367	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	5	65	with	associations	1131:1142	arg1	disease					1167:1173	maternal allergic disease	1149:1173	maternal allergic disease	1149:1173	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	4	66	dep	ranging	724:730	arg1	median					763:768	median	763:768	median	763:768	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	4	67	theme	infant	976:981	arg1	factors					983:989	maternal and infant factors	963:989	maternal and infant factors	963:989	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	4	68	theme	days	746:749	arg1	postpartum					751:760	76 days postpartum	743:760	76 days postpartum	743:760	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	1	69	theme	milk	137:140	arg1	HMOs					160:163	HMOs	160:163	HMOs	160:163	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	69	theme	milk	137:140	arg1	oligosaccharides					142:157	Human milk oligosaccharides	131:157	Human milk oligosaccharides (HMOs)	131:164	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	1	69	theme	milk	137:140	arg1	carbohydrates					186:198	multifunctional carbohydrates	170:198	multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants	170:367	Human milk oligosaccharides (HMOs) are multifunctional carbohydrates naturally present in human milk that act as prebiotics, prevent pathogen binding and infections, modulate the immune system and may support brain development in infants.					
31212920	4	70	theme	Brazilian	831:839	arg1	women					841:845	breastfeeding Brazilian women	817:845	breastfeeding Brazilian women	817:845	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	5	71	attach	presented	1055:1063	arg2	concentrations					1040:1053	HMOs concentrations	1035:1053	HMOs concentrations	1035:1053	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	5	71	attach	presented	1055:1063	arg1	women					1092:1096	women	1092:1096	women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex	1092:1236	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	7	72	theme	specific	1559:1566	arg1	HMOs					1568:1571	specific HMOs	1559:1571	specific HMOs	1559:1571	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	5	73	with	women	1092:1096	arg1	phenotype					1117:1125	the same SeLe phenotype	1103:1125	the same SeLe phenotype	1103:1125	HMOs concentrations presented a high variability even in women with the same SeLe phenotype and associations with maternal allergic disease, time postpartum and with infant's weight, weight gain and sex.					
31212920	2	74	from	differences	414:424	arg1	concentrations					434:447	HMOs concentrations	429:447	HMOs concentrations	429:447	HMOs composition is very individualized and differences in HMOs concentrations may affect the infant's health.					
31212920	7	75	theme	higher	1534:1539	arg1	concentrations					1541:1554	higher concentrations	1534:1554	higher concentrations of specific HMOs	1534:1571	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	0	76	theme	Oligosaccharides	55:70	arg1	Concentrations					72:85	Human Milk Oligosaccharides Concentrations	44:85	Human Milk Oligosaccharides Concentrations	44:85	Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes.					
31212920	7	77	theme	HMOs	1446:1449	arg1	composition					1451:1461	HMOs composition	1446:1461	HMOs composition attributed to maternal SeLe phenotype	1446:1499	Differences in HMOs composition attributed to maternal SeLe phenotype do not impact infant growth, but higher concentrations of specific HMOs may protect against excessive weight gain.					
31212920	0	78	theme	Infant	13:18	arg1	Factors					20:26	Maternal and Infant Factors	0:26	Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations	0:85	Maternal and Infant Factors Associated with Human Milk Oligosaccharides Concentrations According to Secretor and Lewis Phenotypes.					
31212920	2	79	theme	HMOs	370:373	arg1	composition					375:385	HMOs composition	370:385	HMOs composition	370:385	HMOs composition is very individualized and differences in HMOs concentrations may affect the infant's health.					
31212920	4	80	theme	HMOs	996:999	arg1	concentrations					1001:1014	HMOs concentrations	996:1014	HMOs concentrations	996:1014	In this cross-sectional, observational study, 78 single human milk samples ranging from 17 to 76 days postpartum (median: 32 days, IQR: 25-46 days) were collected from breastfeeding Brazilian women, analyzed for 16 representative HMOs by liquid chromatography coupled to mass spectrometry and associations between maternal and infant factors with HMOs concentrations were investigated.					
31212920	6	81	theme	breastfeeding	1306:1318	arg1	women					1330:1334	breastfeeding Brazilian women	1306:1334	breastfeeding Brazilian women	1306:1334	Overall, we present unprecedented data on HMOs concentrations from breastfeeding Brazilian women and novel associations of maternal allergic disease and infant's sex with HMOs concentrations.					
30638239	2	0	from	potential	389:397	arg1	diseases					434:441	digestive diseases	424:441	digestive diseases	424:441	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	2	1	theme	polysaccharide	317:330	arg1	Lentinan					282:289	Lentinan	282:289	Lentinan (LNT)	282:295	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	2	1	theme	polysaccharide	317:330	arg1	type					300:303	a type	298:303	a type of mushroom polysaccharide	298:330	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	1	2	from	development	218:228	arg1	cells					238:242	host cells	233:242	host cells	233:242	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	9	3	theme	protein-coupled	1587:1601	arg1	receptor					1649:1656	a short chain fatty acid receptor	1624:1656	a short chain fatty acid receptor	1624:1656	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	9	3	theme	protein-coupled	1587:1601	arg1	receptor					1603:1610	G protein-coupled receptor 41	1585:1613	G protein-coupled receptor 41 (GPR41)	1585:1621	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	9	3	theme	protein-coupled	1587:1601	arg1	GPR41					1616:1620	GPR41	1616:1620	GPR41	1616:1620	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	7	4	theme	intestinal	1015:1024	arg1	morphology					1026:1035	LNT improved intestinal morphology	1002:1035	LNT improved intestinal morphology	1002:1035	LNT improved intestinal morphology and barrier function.					
30638239	3	5	theme	intestinal	535:544	arg1	injury					546:551	Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury	485:551	Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury	485:551	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	8	6	from	expression	1340:1349	arg1	intestine					1360:1368	small intestine	1354:1368	small intestine	1354:1368	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	7	theme	signaling	1091:1099	arg1	pathways					1101:1108	inflammatory signaling pathways	1078:1108	inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein)	1078:1185	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	7	theme	signaling	1091:1099	arg1	receptor					1121:1128	toll-like receptor 4	1111:1130	toll-like receptor 4	1111:1130	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	7	theme	signaling	1091:1099	arg1	domain					1171:1176	nucleotide binding oligomerization domain	1136:1176	nucleotide binding oligomerization domain	1136:1176	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	4	8	theme	factorial	692:700	arg1	design					702:707	a 2 × 2 factorial design	684:707	a 2 × 2 factorial design	684:707	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	12	9	theme	acid	2085:2088	arg1	synthesis					2090:2098	the short chain fatty acid synthesis	2063:2098	the short chain fatty acid synthesis	2063:2098	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	7	10	theme	LNT	1002:1004	arg1	morphology					1026:1035	LNT improved intestinal morphology	1002:1035	LNT improved intestinal morphology	1002:1035	LNT improved intestinal morphology and barrier function.					
30638239	10	11	theme	Bacterial	1659:1667	arg1	gene					1678:1681	Bacterial 16S rRNA gene	1659:1681	Bacterial 16S rRNA gene pyrosequencing	1659:1696	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	8	12	theme	pro-inflammatory	1191:1206	arg1	factor-α					1234:1241	tumor necrosis factor-α	1219:1241	tumor necrosis factor-α	1219:1241	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	12	theme	pro-inflammatory	1191:1206	arg1	interleukin-6					1263:1275	interleukin-6	1263:1275	interleukin-6	1263:1275	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	12	theme	pro-inflammatory	1191:1206	arg1	cytokines					1208:1216	pro-inflammatory cytokines	1191:1216	pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6)	1191:1276	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	12	theme	pro-inflammatory	1191:1206	arg1	interleukin-1β					1244:1257	interleukin-1β	1244:1257	interleukin-1β	1244:1257	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	3	13	theme	coli	497:500	arg1	LPS					522:524	LPS	522:524	LPS	522:524	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	3	13	theme	coli	497:500	arg1	lipopolysaccharide					502:519	Escherichia coli lipopolysaccharide	485:519	Escherichia coli lipopolysaccharide (LPS)	485:525	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	12	14	theme	intestinal	2014:2023	arg1	composition					2036:2046	intestinal microbiota composition	2014:2046	intestinal microbiota composition	2014:2046	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	9	15	theme	short	1626:1630	arg1	acid					1644:1647	a short chain fatty acid	1624:1647	a short chain fatty acid receptor	1624:1656	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	12	16	theme	chain	2073:2077	arg1	acid					2085:2088	the short chain fatty acid	2063:2088	the short chain fatty acid synthesis	2063:2098	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	10	17	theme	great	1720:1724	arg1	impact					1726:1731	a great impact	1718:1731	a great impact on gut microbiota composition	1718:1761	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	8	18	theme	binding	1147:1153	arg1	pathways					1101:1108	inflammatory signaling pathways	1078:1108	inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein)	1078:1185	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	18	theme	binding	1147:1153	arg1	domain					1171:1176	nucleotide binding oligomerization domain	1136:1176	nucleotide binding oligomerization domain	1136:1176	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	9	19	theme	fatty	1638:1642	arg1	acid					1644:1647	a short chain fatty acid	1624:1647	a short chain fatty acid receptor	1624:1656	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	8	20	theme	necrosis	1225:1232	arg1	factor-α					1234:1241	tumor necrosis factor-α	1219:1241	tumor necrosis factor-α	1219:1241	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	20	theme	necrosis	1225:1232	arg1	cytokines					1208:1216	pro-inflammatory cytokines	1191:1216	pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6)	1191:1276	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	1	21	theme	intestinal	130:139	arg1	microbiota					141:150	The intestinal microbiota	126:150	The intestinal microbiota	126:150	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	1	21	theme	intestinal	130:139	arg1	modifiable					252:261	modifiable	252:261	modifiable	252:261	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	12	22	theme	histones	2181:2188	arg1	hyper-acetylation					2160:2176	hyper-acetylation	2160:2176	hyper-acetylation	2160:2176	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	12	22	theme	histones	2181:2188	arg1	inflammation					2143:2154	inflammation	2143:2154	inflammation	2143:2154	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	10	23	theme	microbiota	1740:1749	arg1	composition					1751:1761	gut microbiota composition	1736:1761	gut microbiota composition	1736:1761	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	3	24	theme	microbiota	614:623	arg1	metabolites					588:598	metabolites	588:598	metabolites	588:598	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	3	24	theme	microbiota	614:623	arg1	composition					572:582	composition	572:582	composition	572:582	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	1	25	from	metabolism	174:183	arg1	cells					238:242	host cells	233:242	host cells	233:242	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	5	26	theme	basal	839:843	arg1	diet					852:855	basal or LNT diet	839:855	basal or LNT diet	839:855	After feeding basal or LNT diet for 21 days, pigs were injected with LPS or saline.					
30638239	6	27	theme	post-injection	916:929	arg1	h					914:914	4 h post-injection	912:929	4 h post-injection	912:929	At 4 h post-injection, pigs were killed and jejunum, ileum and cecal digesta were collected.					
30638239	4	28	theme	×	688:688	arg1	design					702:707	a 2 × 2 factorial design	684:707	a 2 × 2 factorial design	684:707	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	0	29	theme	piglet	77:82	arg1	model					84:88	a piglet model	75:88	a piglet model challenged with lipopolysaccharide	75:123	Lentinan modulates intestinal microbiota and enhances barrier integrity in a piglet model challenged with lipopolysaccharide.					
30638239	5	30	theme	LNT	848:850	arg1	diet					852:855	basal or LNT diet	839:855	basal or LNT diet	839:855	After feeding basal or LNT diet for 21 days, pigs were injected with LPS or saline.					
30638239	12	31	theme	fatty	2079:2083	arg1	acid					2085:2088	the short chain fatty acid	2063:2088	the short chain fatty acid synthesis	2063:2098	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	10	32	theme	rRNA	1673:1676	arg1	gene					1678:1681	Bacterial 16S rRNA gene	1659:1681	Bacterial 16S rRNA gene pyrosequencing	1659:1696	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	8	33	dep	cytokines	1208:1216	arg1	factor-α					1234:1241	tumor necrosis factor-α	1219:1241	tumor necrosis factor-α	1219:1241	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	33	dep	cytokines	1208:1216	arg1	cytokines					1208:1216	pro-inflammatory cytokines	1191:1216	pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6)	1191:1276	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	33	dep	cytokines	1208:1216	arg1	interleukin-6					1263:1275	interleukin-6	1263:1275	interleukin-6	1263:1275	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	33	dep	cytokines	1208:1216	arg1	interleukin-1β					1244:1257	interleukin-1β	1244:1257	interleukin-1β	1244:1257	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	2	34	from	diseases	434:441	arg1	potential					389:397	the potential	385:397	the potential of therapeutic effect on digestive diseases	385:441	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	10	35	from	impact	1726:1731	arg1	composition					1751:1761	gut microbiota composition	1736:1761	gut microbiota composition	1736:1761	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	6	36	theme	cecal	972:976	arg1	digesta					978:984	cecal digesta	972:984	cecal digesta	972:984	At 4 h post-injection, pigs were killed and jejunum, ileum and cecal digesta were collected.					
30638239	2	37	theme	digestive	424:432	arg1	diseases					434:441	digestive diseases	424:441	digestive diseases	424:441	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	11	38	theme	correlation	1808:1818	arg1	analysis					1820:1827	the correlation analysis	1804:1827	the correlation analysis	1804:1827	Moreover, the correlation analysis revealed some potential relationships between cecal metabolites and certain intestinal microbiota.					
30638239	1	39	theme	dietary	266:272	arg1	change					274:279	dietary change	266:279	dietary change	266:279	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	2	40	theme	effect	414:419	arg1	potential					389:397	the potential	385:397	the potential of therapeutic effect on digestive diseases	385:441	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	3	41	theme	intestinal	603:612	arg1	microbiota					614:623	intestinal microbiota	603:623	intestinal microbiota	603:623	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	8	42	theme	heat	1318:1321	arg1	protein					1329:1335	the heat shock protein 70	1314:1338	the heat shock protein 70 expression in small intestine	1314:1368	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	4	43	dep	challenge	798:806	arg1	LPS					809:811	LPS	809:811	LPS	809:811	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	4	43	dep	challenge	798:806	arg1	saline					816:821	saline	816:821	saline	816:821	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	12	44	theme	microbiota	2025:2034	arg1	composition					2036:2046	intestinal microbiota composition	2014:2046	intestinal microbiota composition	2014:2046	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	10	45	theme	taxonomic	1776:1784	arg1	levels					1786:1791	different taxonomic levels	1766:1791	different taxonomic levels	1766:1791	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	8	46	theme	protein	1329:1335	arg1	expression					1340:1349	the heat shock protein 70 expression	1314:1349	the heat shock protein 70 expression in small intestine	1314:1368	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	11	47	theme	potential	1843:1851	arg1	relationships					1853:1865	some potential relationships	1838:1865	some potential relationships between cecal metabolites and certain intestinal microbiota	1838:1925	Moreover, the correlation analysis revealed some potential relationships between cecal metabolites and certain intestinal microbiota.					
30638239	0	48	theme	intestinal	19:28	arg1	microbiota					30:39	intestinal microbiota	19:39	intestinal microbiota	19:39	Lentinan modulates intestinal microbiota and enhances barrier integrity in a piglet model challenged with lipopolysaccharide.					
30638239	9	49	theme	histone	1526:1532	arg1	acetylation					1534:1544	histone acetylation	1526:1544	histone acetylation	1526:1544	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	8	50	dep	receptor	1121:1128	arg1	protein					1178:1184	protein	1178:1184	protein	1178:1184	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	4	51	theme	immunological	784:796	arg1	challenge					798:806	immunological challenge	784:806	immunological challenge (LPS or saline)	784:822	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	2	52	theme	mushroom	308:315	arg1	polysaccharide					317:330	mushroom polysaccharide	308:330	mushroom polysaccharide	308:330	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	0	53	theme	barrier	54:60	arg1	integrity					62:70	barrier integrity	54:70	barrier integrity	54:70	Lentinan modulates intestinal microbiota and enhances barrier integrity in a piglet model challenged with lipopolysaccharide.					
30638239	10	54	theme	different	1766:1774	arg1	levels					1786:1791	different taxonomic levels	1766:1791	different taxonomic levels	1766:1791	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	8	55	theme	small	1354:1358	arg1	intestine					1360:1368	small intestine	1354:1368	small intestine	1354:1368	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	1	56	theme	vital	160:164	arg1	role					166:169	a vital role	158:169	a vital role	158:169	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	4	57	theme	LNT	770:772	arg1	diet					774:777	basal or LNT diet	761:777	basal or LNT diet	761:777	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	4	57	theme	LNT	770:772	arg1	treatment					750:758	a dietary treatment	740:758	a dietary treatment (basal or LNT diet)	740:778	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	4	58	theme	weaned	656:661	arg1	piglets					663:669	Twenty-four weaned piglets	644:669	Twenty-four weaned piglets	644:669	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	12	59	theme	intestinal	1969:1978	arg1	health					1980:1985	intestinal health	1969:1985	intestinal health	1969:1985	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	3	60	theme	Escherichia	485:495	arg1	LPS					522:524	LPS	522:524	LPS	522:524	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	3	60	theme	Escherichia	485:495	arg1	lipopolysaccharide					502:519	Escherichia coli lipopolysaccharide	485:519	Escherichia coli lipopolysaccharide (LPS)	485:525	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	9	61	from	isovalerate	1457:1467	arg1	digesta					1478:1484	cecal digesta	1472:1484	cecal digesta	1472:1484	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	7	62	theme	improved	1006:1013	arg1	morphology					1026:1035	LNT improved intestinal morphology	1002:1035	LNT improved intestinal morphology	1002:1035	LNT improved intestinal morphology and barrier function.					
30638239	3	63	theme	-induced	526:533	arg1	injury					546:551	Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury	485:551	Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury	485:551	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	9	64	theme	receptor	1603:1610	arg1	level					1576:1580	the protein level	1564:1580	the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor	1564:1656	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	9	65	from	isobutyrate	1441:1451	arg1	digesta					1478:1484	cecal digesta	1472:1484	cecal digesta	1472:1484	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	2	66	dep	Lentinan	282:289	arg1	LNT					292:294	LNT	292:294	LNT	292:294	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	10	67	theme	16S	1669:1671	arg1	gene					1678:1681	Bacterial 16S rRNA gene	1659:1681	Bacterial 16S rRNA gene pyrosequencing	1659:1696	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	3	68	dep	composition	572:582	arg1	the					568:570	the	568:570	the	568:570	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	8	69	theme	inflammatory	1078:1089	arg1	pathways					1101:1108	inflammatory signaling pathways	1078:1108	inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein)	1078:1185	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	69	theme	inflammatory	1078:1089	arg1	receptor					1121:1128	toll-like receptor 4	1111:1130	toll-like receptor 4	1111:1130	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	69	theme	inflammatory	1078:1089	arg1	domain					1171:1176	nucleotide binding oligomerization domain	1136:1176	nucleotide binding oligomerization domain	1136:1176	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	9	70	from	butyrate	1431:1438	arg1	digesta					1478:1484	cecal digesta	1472:1484	cecal digesta	1472:1484	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	8	71	theme	oligomerization	1155:1169	arg1	pathways					1101:1108	inflammatory signaling pathways	1078:1108	inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein)	1078:1185	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	71	theme	oligomerization	1155:1169	arg1	domain					1171:1176	nucleotide binding oligomerization domain	1136:1176	nucleotide binding oligomerization domain	1136:1176	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	10	72	theme	gene	1678:1681	arg1	pyrosequencing					1683:1696	Bacterial 16S rRNA gene pyrosequencing	1659:1696	Bacterial 16S rRNA gene pyrosequencing	1659:1696	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	1	73	theme	pathogen	186:193	arg1	resistance					195:204	pathogen resistance	186:204	pathogen resistance	186:204	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	8	74	theme	toll-like	1111:1119	arg1	pathways					1101:1108	inflammatory signaling pathways	1078:1108	inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein)	1078:1185	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	74	theme	toll-like	1111:1119	arg1	receptor					1121:1128	toll-like receptor 4	1111:1130	toll-like receptor 4	1111:1130	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	11	75	theme	certain	1897:1903	arg1	microbiota					1916:1925	certain intestinal microbiota	1897:1925	certain intestinal microbiota	1897:1925	Moreover, the correlation analysis revealed some potential relationships between cecal metabolites and certain intestinal microbiota.					
30638239	1	76	theme	immune	211:216	arg1	development					218:228	immune development	211:228	immune development	211:228	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	9	77	theme	chain	1632:1636	arg1	acid					1644:1647	a short chain fatty acid	1624:1647	a short chain fatty acid receptor	1624:1656	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	12	78	theme	short	2067:2071	arg1	acid					2085:2088	the short chain fatty acid	2063:2088	the short chain fatty acid synthesis	2063:2098	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	4	79	used	used	676:679	arg2	piglets					663:669	Twenty-four weaned piglets	644:669	Twenty-four weaned piglets	644:669	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	9	80	theme	propionate	1419:1428	arg1	concentrations					1401:1414	the concentrations	1397:1414	the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta	1397:1484	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	8	81	theme	nucleotide	1136:1145	arg1	pathways					1101:1108	inflammatory signaling pathways	1078:1108	inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein)	1078:1185	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	81	theme	nucleotide	1136:1145	arg1	domain					1171:1176	nucleotide binding oligomerization domain	1136:1176	nucleotide binding oligomerization domain	1136:1176	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	9	82	theme	acid	1644:1647	arg1	receptor					1603:1610	G protein-coupled receptor 41	1585:1613	G protein-coupled receptor 41 (GPR41)	1585:1621	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	9	82	theme	acid	1644:1647	arg1	receptor					1649:1656	a short chain fatty acid receptor	1624:1656	a short chain fatty acid receptor	1624:1656	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	8	83	theme	tumor	1219:1223	arg1	factor-α					1234:1241	tumor necrosis factor-α	1219:1241	tumor necrosis factor-α	1219:1241	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	83	theme	tumor	1219:1223	arg1	cytokines					1208:1216	pro-inflammatory cytokines	1191:1216	pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6)	1191:1276	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	4	84	theme	basal	761:765	arg1	diet					774:777	basal or LNT diet	761:777	basal or LNT diet	761:777	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	4	84	theme	basal	761:765	arg1	treatment					750:758	a dietary treatment	740:758	a dietary treatment (basal or LNT diet)	740:778	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	9	85	from	increase	1514:1521	arg1	acetylation					1534:1544	histone acetylation	1526:1544	histone acetylation	1526:1544	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	10	86	theme	gut	1736:1738	arg1	composition					1751:1761	gut microbiota composition	1736:1761	gut microbiota composition	1736:1761	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	1	87	from	resistance	195:204	arg1	cells					238:242	host cells	233:242	host cells	233:242	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	2	88	from	effect	414:419	arg1	diseases					434:441	digestive diseases	424:441	digestive diseases	424:441	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	9	89	theme	cecal	1472:1476	arg1	digesta					1478:1484	cecal digesta	1472:1484	cecal digesta	1472:1484	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	4	90	theme	dietary	742:748	arg1	diet					774:777	basal or LNT diet	761:777	basal or LNT diet	761:777	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	4	90	theme	dietary	742:748	arg1	treatment					750:758	a dietary treatment	740:758	a dietary treatment (basal or LNT diet)	740:778	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	2	91	theme	intestinal	356:365	arg1	inflammation					367:378	intestinal inflammation	356:378	intestinal inflammation	356:378	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	3	92	from	composition	572:582	arg1	model					637:641	a piglet model	628:641	a piglet model	628:641	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	9	93	theme	significant	1502:1512	arg1	increase					1514:1521	a significant increase	1500:1521	a significant increase in histone acetylation	1500:1544	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	2	94	theme	therapeutic	402:412	arg1	effect					414:419	therapeutic effect	402:419	therapeutic effect on digestive diseases	402:441	Lentinan (LNT), a type of mushroom polysaccharide, is known to ameliorate intestinal inflammation with the potential of therapeutic effect on digestive diseases.					
30638239	4	95	theme	main	718:721	arg1	factors					723:729	the main factors	714:729	the main factors	714:729	Twenty-four weaned piglets were used in a 2 × 2 factorial design, and the main factors included a dietary treatment (basal or LNT diet) and immunological challenge (LPS or saline).					
30638239	3	96	from	metabolites	588:598	arg1	model					637:641	a piglet model	628:641	a piglet model	628:641	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	11	97	theme	cecal	1875:1879	arg1	metabolites					1881:1891	cecal metabolites	1875:1891	cecal metabolites	1875:1891	Moreover, the correlation analysis revealed some potential relationships between cecal metabolites and certain intestinal microbiota.					
30638239	3	98	theme	piglet	630:635	arg1	model					637:641	a piglet model	628:641	a piglet model	628:641	We hypothesized that LNT could alleviate Escherichia coli lipopolysaccharide (LPS)-induced intestinal injury via regulating the composition and metabolites of intestinal microbiota in a piglet model.					
30638239	8	99	theme	shock	1323:1327	arg1	protein					1329:1335	the heat shock protein 70	1314:1338	the heat shock protein 70 expression in small intestine	1314:1368	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	7	100	theme	barrier	1041:1047	arg1	function					1049:1056	barrier function	1041:1056	barrier function	1041:1056	LNT improved intestinal morphology and barrier function.					
30638239	11	101	theme	intestinal	1905:1914	arg1	microbiota					1916:1925	certain intestinal microbiota	1897:1925	certain intestinal microbiota	1897:1925	Moreover, the correlation analysis revealed some potential relationships between cecal metabolites and certain intestinal microbiota.					
30638239	8	102	dep	pathways	1101:1108	arg1	pathways					1101:1108	inflammatory signaling pathways	1078:1108	inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein)	1078:1185	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	102	dep	pathways	1101:1108	arg1	expression					1278:1287	expression	1278:1287	expression	1278:1287	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	102	dep	pathways	1101:1108	arg1	receptor					1121:1128	toll-like receptor 4	1111:1130	toll-like receptor 4	1111:1130	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	8	102	dep	pathways	1101:1108	arg1	domain					1171:1176	nucleotide binding oligomerization domain	1136:1176	nucleotide binding oligomerization domain	1136:1176	LNT also inhibited inflammatory signaling pathways (toll-like receptor 4 and nucleotide binding oligomerization domain protein) and pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β and interleukin-6) expression, as well as up-regulated the heat shock protein 70 expression in small intestine.					
30638239	12	103	from	reduction	2130:2138	arg1	hyper-acetylation					2160:2176	hyper-acetylation	2160:2176	hyper-acetylation	2160:2176	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	12	103	from	reduction	2130:2138	arg1	inflammation					2143:2154	inflammation	2143:2154	inflammation	2143:2154	These results indicate that LNT promotes intestinal health, in part, through altering intestinal microbiota composition and increasing the short chain fatty acid synthesis, which subsequently lead to a reduction in inflammation and hyper-acetylation of histones.					
30638239	1	104	theme	host	233:236	arg1	cells					238:242	host cells	233:242	host cells	233:242	The intestinal microbiota plays a vital role in metabolism, pathogen resistance, and immune development in host cells, and is modifiable by dietary change.					
30638239	9	105	theme	protein	1568:1574	arg1	level					1576:1580	the protein level	1564:1580	the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor	1564:1656	In addition, LNT enhanced the concentrations of propionate, butyrate, isobutyrate and isovalerate in cecal digesta, resulting in a significant increase in histone acetylation without affecting the protein level of G protein-coupled receptor 41 (GPR41), a short chain fatty acid receptor.					
30638239	10	106	contain	had	1714:1716	arg2	impact					1726:1731	a great impact	1718:1731	a great impact on gut microbiota composition	1718:1761	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
30638239	10	106	contain	had	1714:1716	arg1	LNT					1710:1712	LNT	1710:1712	LNT	1710:1712	Bacterial 16S rRNA gene pyrosequencing showed that LNT had a great impact on gut microbiota composition at different taxonomic levels.					
29324762	6	0	theme	energy	958:963	arg1	metabolites					965:975	energy metabolites	958:975	energy metabolites	958:975	Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members.					
29324762	3	1	theme	kcal	456:459	arg1	sucrose					473:479	45%kcal fat/17%kcal sucrose	453:479	45%kcal fat/17%kcal sucrose	453:479	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	1	theme	kcal	456:459	arg1	diet					447:450	control or high fat/sugar diet	421:450	diet	447:450	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	6	2	theme	complex	924:930	arg1	lipids					932:937	complex lipids	924:937	complex lipids	924:937	Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members.					
29324762	8	3	theme	kidney	1212:1217	arg1	tissues					1229:1235	lung, kidney and liver tissues	1206:1235	lung, kidney and liver tissues	1206:1235	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	3	4	theme	fat/17	461:466	arg1	sucrose					473:479	45%kcal fat/17%kcal sucrose	453:479	45%kcal fat/17%kcal sucrose	453:479	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	4	theme	fat/17	461:466	arg1	diet					447:450	control or high fat/sugar diet	421:450	diet	447:450	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	4	5	theme	lung	668:671	arg1	metabolism					673:682	lung metabolism	668:682	lung metabolism resulting from obesity and diet composition	668:726	We performed untargeted metabolomics by GC-TOFMS and HILIC-QTOFMS and lipidomics by RPLC-QTOFMS to reveal global changes in lung metabolism resulting from obesity and diet composition.					
29324762	4	6	theme	global	650:655	arg1	changes					657:663	global changes	650:663	global changes in lung metabolism resulting from obesity and diet composition	650:726	We performed untargeted metabolomics by GC-TOFMS and HILIC-QTOFMS and lipidomics by RPLC-QTOFMS to reveal global changes in lung metabolism resulting from obesity and diet composition.					
29324762	5	7	theme	mouse	837:841	arg1	tissues					848:854	mouse lung tissues	837:854	mouse lung tissues	837:854	From a total of 447 detected metabolites, we found 91 metabolite and lipid species significantly altered in mouse lung tissues upon dietary treatments.					
29324762	9	8	theme	lung	1457:1460	arg1	metabolome					1462:1471	the lung metabolome	1453:1471	the lung metabolome	1453:1471	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	9	9	from	effects	1427:1433	arg1	composition					1438:1448	composition	1438:1448	composition of the lung metabolome	1438:1471	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	1	10	theme	accompanying	55:66	arg1	disease					78:84	accompanying metabolic disease	55:84	accompanying metabolic disease	55:84	Obesity and accompanying metabolic disease is negatively correlated with lung health yet the exact mechanisms by which obesity affects the lung are not well characterized.					
29324762	6	11	theme	altered	895:901	arg1	metabolites					903:913	Significantly altered metabolites	881:913	Significantly altered metabolites	881:913	Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members.					
29324762	1	12	theme	lung	116:119	arg1	health					121:126	lung health	116:126	lung health	116:126	Obesity and accompanying metabolic disease is negatively correlated with lung health yet the exact mechanisms by which obesity affects the lung are not well characterized.					
29324762	3	13	theme	fat/sugar	437:445	arg1	diet					447:450	control or high fat/sugar diet	421:450	diet	447:450	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	13	theme	fat/sugar	437:445	arg1	sucrose					473:479	45%kcal fat/17%kcal sucrose	453:479	45%kcal fat/17%kcal sucrose	453:479	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	1	14	theme	metabolic	68:76	arg1	disease					78:84	accompanying metabolic disease	55:84	accompanying metabolic disease	55:84	Obesity and accompanying metabolic disease is negatively correlated with lung health yet the exact mechanisms by which obesity affects the lung are not well characterized.					
29324762	5	15	theme	metabolites	758:768	arg1	total					736:740	a total	734:740	a total of 447 detected metabolites	734:768	From a total of 447 detected metabolites, we found 91 metabolite and lipid species significantly altered in mouse lung tissues upon dietary treatments.					
29324762	5	16	theme	lipid	798:802	arg1	species					804:810	lipid species	798:810	lipid species	798:810	From a total of 447 detected metabolites, we found 91 metabolite and lipid species significantly altered in mouse lung tissues upon dietary treatments.					
29324762	4	17	from	changes	657:663	arg1	metabolism					673:682	lung metabolism	668:682	lung metabolism resulting from obesity and diet composition	668:726	We performed untargeted metabolomics by GC-TOFMS and HILIC-QTOFMS and lipidomics by RPLC-QTOFMS to reveal global changes in lung metabolism resulting from obesity and diet composition.					
29324762	8	18	theme	metabolic	1251:1259	arg1	changes					1261:1267	few metabolic changes	1247:1267	few metabolic changes	1247:1267	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	6	19	theme	adenosine	994:1002	arg1	members					1020:1026	adenosine and NAD pathway members	994:1026	adenosine and NAD pathway members	994:1026	Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members.					
29324762	9	20	theme	lung-specific	1521:1533	arg1	pathways					1535:1542	lung-specific pathways	1521:1542	lung-specific pathways	1521:1542	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	3	21	theme	fat	496:498	arg1	sucrose					521:527	60%kcal fat/7% sucrose	506:527	60%kcal fat/7% sucrose	506:527	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	21	theme	fat	496:498	arg1	diet					500:503	very high fat diet	486:503	very high fat diet (60%kcal fat/7% sucrose)	486:528	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	8	22	theme	metabolic	1334:1342	arg1	organ					1344:1348	an independent metabolic organ	1319:1348	an independent metabolic organ	1319:1348	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	8	22	theme	metabolic	1334:1342	arg1	lung					1311:1314	the lung	1307:1314	the lung	1307:1314	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	6	23	theme	amino	978:982	arg1	acids					984:988	amino acids	978:988	amino acids	978:988	Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members.					
29324762	0	24	theme	Obesogenic	0:9	arg1	diets					11:15	Obesogenic diets	0:15	Obesogenic diets	0:15	Obesogenic diets alter metabolism in mice.					
29324762	5	25	theme	lung	843:846	arg1	tissues					848:854	mouse lung tissues	837:854	mouse lung tissues	837:854	From a total of 447 detected metabolites, we found 91 metabolite and lipid species significantly altered in mouse lung tissues upon dietary treatments.					
29324762	2	26	theme	lung	354:357	arg1	metabolism					359:368	lung metabolism	354:368	lung metabolism	354:368	Since obesity is associated with lung diseases as chronic bronchitis and asthma, we designed a series of experiments to measure changes in lung metabolism in mice fed obesogenic diets.					
29324762	4	27	theme	diet	711:714	arg1	composition					716:726	diet composition	711:726	diet composition	711:726	We performed untargeted metabolomics by GC-TOFMS and HILIC-QTOFMS and lipidomics by RPLC-QTOFMS to reveal global changes in lung metabolism resulting from obesity and diet composition.					
29324762	8	28	theme	lung	1206:1209	arg1	tissues					1229:1235	lung, kidney and liver tissues	1206:1235	lung, kidney and liver tissues	1206:1235	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	2	29	from	changes	343:349	arg1	mice					373:376	mice	373:376	mice fed obesogenic diets	373:397	Since obesity is associated with lung diseases as chronic bronchitis and asthma, we designed a series of experiments to measure changes in lung metabolism in mice fed obesogenic diets.					
29324762	2	29	from	changes	343:349	arg1	metabolism					359:368	lung metabolism	354:368	lung metabolism	354:368	Since obesity is associated with lung diseases as chronic bronchitis and asthma, we designed a series of experiments to measure changes in lung metabolism in mice fed obesogenic diets.					
29324762	8	30	theme	liver	1223:1227	arg1	tissues					1229:1235	lung, kidney and liver tissues	1206:1235	lung, kidney and liver tissues	1206:1235	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	9	31	theme	disease	1498:1504	arg1	progression					1506:1516	disease progression	1498:1516	disease progression	1498:1516	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	3	32	theme	high	432:435	arg1	diet					447:450	control or high fat/sugar diet	421:450	diet	447:450	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	32	theme	high	432:435	arg1	sucrose					473:479	45%kcal fat/17%kcal sucrose	453:479	45%kcal fat/17%kcal sucrose	453:479	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	7	33	theme	obese	1073:1077	arg1	groups					1079:1084	both obese groups	1068:1084	both obese groups	1068:1084	While some metabolites were altered in both obese groups compared to control, others were different between obesogenic diet groups.					
29324762	2	34	theme	chronic	265:271	arg1	bronchitis					273:282	chronic bronchitis	265:282	chronic bronchitis	265:282	Since obesity is associated with lung diseases as chronic bronchitis and asthma, we designed a series of experiments to measure changes in lung metabolism in mice fed obesogenic diets.					
29324762	3	35	theme	high	491:494	arg1	sucrose					521:527	60%kcal fat/7% sucrose	506:527	60%kcal fat/7% sucrose	506:527	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	35	theme	high	491:494	arg1	diet					500:503	very high fat diet	486:503	very high fat diet (60%kcal fat/7% sucrose)	486:528	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	1	36	theme	exact	136:140	arg1	mechanisms					142:151	the exact mechanisms	132:151	the exact mechanisms by which obesity affects the lung	132:185	Obesity and accompanying metabolic disease is negatively correlated with lung health yet the exact mechanisms by which obesity affects the lung are not well characterized.					
29324762	5	37	theme	dietary	861:867	arg1	treatments					869:878	dietary treatments	861:878	dietary treatments	861:878	From a total of 447 detected metabolites, we found 91 metabolite and lipid species significantly altered in mouse lung tissues upon dietary treatments.					
29324762	9	38	contain	have	1403:1406	arg1	obesity					1374:1380	obesity	1374:1380	obesity	1374:1380	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	9	38	contain	have	1403:1406	arg1	composition					1391:1401	diet composition	1386:1401	diet composition	1386:1401	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	9	38	contain	have	1403:1406	arg2	effects					1427:1433	direct mechanistic effects	1408:1433	direct mechanistic effects	1408:1433	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	2	39	theme	obesogenic	382:391	arg1	diets					393:397	obesogenic diets	382:397	obesogenic diets	382:397	Since obesity is associated with lung diseases as chronic bronchitis and asthma, we designed a series of experiments to measure changes in lung metabolism in mice fed obesogenic diets.					
29324762	3	40	theme	%	508:508	arg1	sucrose					521:527	60%kcal fat/7% sucrose	506:527	60%kcal fat/7% sucrose	506:527	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	40	theme	%	508:508	arg1	diet					500:503	very high fat diet	486:503	very high fat diet (60%kcal fat/7% sucrose)	486:528	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	4	41	theme	untargeted	557:566	arg1	metabolomics					568:579	untargeted metabolomics	557:579	untargeted metabolomics by GC-TOFMS and HILIC-QTOFMS	557:608	We performed untargeted metabolomics by GC-TOFMS and HILIC-QTOFMS and lipidomics by RPLC-QTOFMS to reveal global changes in lung metabolism resulting from obesity and diet composition.					
29324762	3	42	theme	control	421:427	arg1	diet					447:450	control or high fat/sugar diet	421:450	diet	447:450	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	42	theme	control	421:427	arg1	sucrose					473:479	45%kcal fat/17%kcal sucrose	453:479	45%kcal fat/17%kcal sucrose	453:479	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	43	theme	kcal	509:512	arg1	sucrose					521:527	60%kcal fat/7% sucrose	506:527	60%kcal fat/7% sucrose	506:527	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	43	theme	kcal	509:512	arg1	diet					500:503	very high fat diet	486:503	very high fat diet (60%kcal fat/7% sucrose)	486:528	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	5	44	theme	detected	749:756	arg1	metabolites					758:768	447 detected metabolites	745:768	447 detected metabolites	745:768	From a total of 447 detected metabolites, we found 91 metabolite and lipid species significantly altered in mouse lung tissues upon dietary treatments.					
29324762	8	45	theme	few	1247:1249	arg1	changes					1261:1267	few metabolic changes	1247:1267	few metabolic changes	1247:1267	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	2	46	theme	experiments	320:330	arg1	series					310:315	a series	308:315	a series of experiments	308:330	Since obesity is associated with lung diseases as chronic bronchitis and asthma, we designed a series of experiments to measure changes in lung metabolism in mice fed obesogenic diets.					
29324762	6	47	theme	pathway	1012:1018	arg1	members					1020:1026	adenosine and NAD pathway members	994:1026	adenosine and NAD pathway members	994:1026	Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members.					
29324762	3	48	theme	45	453:454	arg1	%					455:455	%	455:455	%	455:455	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	9	49	theme	mechanistic	1415:1425	arg1	effects					1427:1433	direct mechanistic effects	1408:1433	direct mechanistic effects	1408:1433	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	3	50	theme	60	506:507	arg1	%					508:508	%	508:508	%	508:508	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	51	theme	%	455:455	arg1	sucrose					473:479	45%kcal fat/17%kcal sucrose	453:479	45%kcal fat/17%kcal sucrose	453:479	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	51	theme	%	455:455	arg1	diet					447:450	control or high fat/sugar diet	421:450	diet	447:450	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	8	52	theme	independent	1322:1332	arg1	organ					1344:1348	an independent metabolic organ	1319:1348	an independent metabolic organ	1319:1348	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	8	52	theme	independent	1322:1332	arg1	lung					1311:1314	the lung	1307:1314	the lung	1307:1314	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	9	53	theme	direct	1408:1413	arg1	effects					1427:1433	direct mechanistic effects	1408:1433	direct mechanistic effects	1408:1433	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	6	54	theme	fatty	945:949	arg1	acids					951:955	free fatty acids	940:955	free fatty acids	940:955	Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members.					
29324762	2	55	theme	lung	248:251	arg1	diseases					253:260	lung diseases	248:260	lung diseases as chronic bronchitis and asthma	248:293	Since obesity is associated with lung diseases as chronic bronchitis and asthma, we designed a series of experiments to measure changes in lung metabolism in mice fed obesogenic diets.					
29324762	6	56	theme	NAD	1008:1010	arg1	members					1020:1026	adenosine and NAD pathway members	994:1026	adenosine and NAD pathway members	994:1026	Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members.					
29324762	6	57	theme	free	940:943	arg1	acids					951:955	free fatty acids	940:955	free fatty acids	940:955	Significantly altered metabolites included complex lipids, free fatty acids, energy metabolites, amino acids and adenosine and NAD pathway members.					
29324762	9	58	theme	diet	1386:1389	arg1	composition					1391:1401	diet composition	1386:1401	diet composition	1386:1401	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	8	59	theme	changes	1190:1196	arg1	comparison					1176:1185	a comparison	1174:1185	a comparison of changes between lung, kidney and liver tissues	1174:1235	Furthermore, a comparison of changes between lung, kidney and liver tissues indicated few metabolic changes were shared across organs, suggesting the lung is an independent metabolic organ.					
29324762	3	60	theme	fat/7	514:518	arg1	sucrose					521:527	60%kcal fat/7% sucrose	506:527	60%kcal fat/7% sucrose	506:527	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	60	theme	fat/7	514:518	arg1	diet					500:503	very high fat diet	486:503	very high fat diet (60%kcal fat/7% sucrose)	486:528	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	61	theme	%	467:467	arg1	sucrose					473:479	45%kcal fat/17%kcal sucrose	453:479	45%kcal fat/17%kcal sucrose	453:479	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	61	theme	%	467:467	arg1	diet					447:450	control or high fat/sugar diet	421:450	diet	447:450	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	7	62	theme	obesogenic	1137:1146	arg1	groups					1153:1158	obesogenic diet groups	1137:1158	obesogenic diet groups	1137:1158	While some metabolites were altered in both obese groups compared to control, others were different between obesogenic diet groups.					
29324762	3	63	theme	%	519:519	arg1	sucrose					521:527	60%kcal fat/7% sucrose	506:527	60%kcal fat/7% sucrose	506:527	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	63	theme	%	519:519	arg1	diet					500:503	very high fat diet	486:503	very high fat diet (60%kcal fat/7% sucrose)	486:528	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	9	64	theme	metabolome	1462:1471	arg1	composition					1438:1448	composition	1438:1448	composition of the lung metabolome	1438:1471	These results indicate obesity and diet composition have direct mechanistic effects on composition of the lung metabolome, which may contribute to disease progression by lung-specific pathways.					
29324762	3	65	theme	kcal	468:471	arg1	sucrose					473:479	45%kcal fat/17%kcal sucrose	453:479	45%kcal fat/17%kcal sucrose	453:479	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	3	65	theme	kcal	468:471	arg1	diet					447:450	control or high fat/sugar diet	421:450	diet	447:450	Mice were fed either control or high fat/sugar diet (45%kcal fat/17%kcal sucrose), or very high fat diet (60%kcal fat/7% sucrose) for 150 days.					
29324762	7	66	theme	diet	1148:1151	arg1	groups					1153:1158	obesogenic diet groups	1137:1158	obesogenic diet groups	1137:1158	While some metabolites were altered in both obese groups compared to control, others were different between obesogenic diet groups.					
30340010	0	0	theme	hydrogel	91:98	arg1	beads					100:104	curcumin-loaded sodium alginate/ZnO hydrogel beads	55:104	curcumin-loaded sodium alginate/ZnO hydrogel beads	55:104	Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads.					
30340010	5	1	theme	unstable	1180:1187	arg1	drugs					1189:1193	unstable drugs	1180:1193	unstable drugs	1180:1193	These results are beneficial for the design of delivery systems to entrap and control the release of unstable drugs.					
30340010	5	2	theme	delivery	1126:1133	arg1	systems					1135:1141	delivery systems	1126:1141	delivery systems	1126:1141	These results are beneficial for the design of delivery systems to entrap and control the release of unstable drugs.					
30340010	0	3	theme	alginate/ZnO	78:89	arg1	beads					100:104	curcumin-loaded sodium alginate/ZnO hydrogel beads	55:104	curcumin-loaded sodium alginate/ZnO hydrogel beads	55:104	Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads.					
30340010	2	4	theme	Cur	611:613	arg1	time					603:606	the residence time	589:606	the residence time of Cur	589:613	The results show that the composite hydrogel beads exhibit good pH sensitivity and controlled-release capacity, which could prolong the residence time of Cur in the gastrointestinal tract.					
30340010	1	5	theme	novel	148:152	arg1	beads					183:187	novel sodium alginate/ZnO hydrogel beads	148:187	novel sodium alginate/ZnO hydrogel beads	148:187	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	5	6	theme	drugs	1189:1193	arg1	release					1169:1175	the release	1165:1175	the release of unstable drugs	1165:1193	These results are beneficial for the design of delivery systems to entrap and control the release of unstable drugs.					
30340010	5	7	theme	systems	1135:1141	arg1	design					1116:1121	the design	1112:1121	the design of delivery systems to entrap and control the release of unstable drugs	1112:1193	These results are beneficial for the design of delivery systems to entrap and control the release of unstable drugs.					
30340010	2	8	theme	gastrointestinal	622:637	arg1	tract					639:643	the gastrointestinal tract	618:643	the gastrointestinal tract	618:643	The results show that the composite hydrogel beads exhibit good pH sensitivity and controlled-release capacity, which could prolong the residence time of Cur in the gastrointestinal tract.					
30340010	2	9	theme	controlled-release	540:557	arg1	capacity					559:566	controlled-release capacity	540:566	controlled-release capacity	540:566	The results show that the composite hydrogel beads exhibit good pH sensitivity and controlled-release capacity, which could prolong the residence time of Cur in the gastrointestinal tract.					
30340010	0	10	theme	beads	100:104	arg1	release					18:24	release	18:24	release	18:24	Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads.					
30340010	0	10	theme	beads	100:104	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads.					
30340010	0	10	theme	beads	100:104	arg1	activity					43:50	antioxidant activity	31:50	antioxidant activity	31:50	Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads.					
30340010	3	11	theme	higher	921:926	arg1	activity					940:947	a higher antioxidant activity	919:947	a higher antioxidant activity	919:947	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	1	12	dep	fabricated	125:134	arg1	optimize					192:199	optimize	192:199	to optimize the release profile of curcumin (Cur)	189:237	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	1	12	dep	fabricated	125:134	arg1	avoid					246:250	avoid	246:250	to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions	243:454	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	1	13	theme	ultraviolet	410:420	arg1	light					427:431	ultraviolet (UV) light	410:431	ultraviolet (UV) light	410:431	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	1	14	theme	sodium	154:159	arg1	beads					183:187	novel sodium alginate/ZnO hydrogel beads	148:187	novel sodium alginate/ZnO hydrogel beads	148:187	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	3	15	theme	UV	664:665	arg1	irradiation					667:677	UV irradiation	664:677	UV irradiation	664:677	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	3	16	theme	antioxidant	928:938	arg1	activity					940:947	a higher antioxidant activity	919:947	a higher antioxidant activity	919:947	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	1	17	theme	alginate/ZnO	161:172	arg1	beads					183:187	novel sodium alginate/ZnO hydrogel beads	148:187	novel sodium alginate/ZnO hydrogel beads	148:187	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	4	18	theme	composite	954:962	arg1	beads					973:977	The composite hydrogel beads	950:977	The composite hydrogel beads	950:977	The composite hydrogel beads protected the Cur from light degradation and can therefore prolong the antioxidant activity of Cur.					
30340010	1	19	theme	UV	423:424	arg1	light					427:431	ultraviolet (UV) light	410:431	ultraviolet (UV) light	410:431	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	4	20	theme	Cur	1074:1076	arg1	activity					1062:1069	the antioxidant activity	1046:1069	the antioxidant activity of Cur	1046:1076	The composite hydrogel beads protected the Cur from light degradation and can therefore prolong the antioxidant activity of Cur.					
30340010	1	21	theme	hydrogel	174:181	arg1	beads					183:187	novel sodium alginate/ZnO hydrogel beads	148:187	novel sodium alginate/ZnO hydrogel beads	148:187	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	3	22	theme	1,1-Diphenyl-2-picrylhydrazyl	692:720	arg1	capacity					740:747	the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity	688:747	the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads	688:776	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	3	23	theme	same	863:866	arg1	conditions					868:877	the same conditions	859:877	the same conditions	859:877	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	1	24	theme	beads	183:187	arg1	series					138:143	a series	136:143	a series of novel sodium alginate/ZnO hydrogel beads	136:187	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	2	25	theme	residence	593:601	arg1	time					603:606	the residence time	589:606	the residence time of Cur	589:613	The results show that the composite hydrogel beads exhibit good pH sensitivity and controlled-release capacity, which could prolong the residence time of Cur in the gastrointestinal tract.					
30340010	4	26	theme	hydrogel	964:971	arg1	beads					973:977	The composite hydrogel beads	950:977	The composite hydrogel beads	950:977	The composite hydrogel beads protected the Cur from light degradation and can therefore prolong the antioxidant activity of Cur.					
30340010	1	27	theme	burst	256:260	arg1	release					262:268	the burst release	252:268	the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions	252:454	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	2	28	theme	hydrogel	493:500	arg1	beads					502:506	the composite hydrogel beads	479:506	the composite hydrogel beads	479:506	The results show that the composite hydrogel beads exhibit good pH sensitivity and controlled-release capacity, which could prolong the residence time of Cur in the gastrointestinal tract.					
30340010	4	29	theme	antioxidant	1050:1060	arg1	activity					1062:1069	the antioxidant activity	1046:1069	the antioxidant activity of Cur	1046:1076	The composite hydrogel beads protected the Cur from light degradation and can therefore prolong the antioxidant activity of Cur.					
30340010	0	30	theme	antioxidant	31:41	arg1	activity					43:50	antioxidant activity	31:50	antioxidant activity	31:50	Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads.					
30340010	2	31	theme	composite	483:491	arg1	beads					502:506	the composite hydrogel beads	479:506	the composite hydrogel beads	479:506	The results show that the composite hydrogel beads exhibit good pH sensitivity and controlled-release capacity, which could prolong the residence time of Cur in the gastrointestinal tract.					
30340010	1	32	theme	Cur	348:350	arg1	clearance					381:389	rapid physiological clearance	361:389	rapid physiological clearance	361:389	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	1	32	theme	Cur	348:350	arg1	sensitivity					395:405	sensitivity	395:405	sensitivity to ultraviolet (UV) light and alkaline solutions	395:454	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	1	32	theme	Cur	348:350	arg1	weaknesses					334:343	the weaknesses	330:343	the weaknesses	330:343	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	1	33	used	used	313:316	arg2	hydrogels					291:299	pure hydrogels	286:299	pure hydrogels	286:299	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	3	34	theme	DPPH	723:726	arg1	capacity					740:747	the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity	688:747	the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads	688:776	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	1	35	theme	release	205:211	arg1	profile					213:219	the release profile	201:219	the release profile of curcumin (Cur)	201:237	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	3	36	theme	scavenging	729:738	arg1	capacity					740:747	the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity	688:747	the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads	688:776	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	2	37	dep	exhibit	508:514	arg1	prolong					581:587	prolong	581:587	could prolong the residence time of Cur in the gastrointestinal tract	575:643	The results show that the composite hydrogel beads exhibit good pH sensitivity and controlled-release capacity, which could prolong the residence time of Cur in the gastrointestinal tract.					
30340010	1	38	theme	alkaline	437:444	arg1	solutions					446:454	alkaline solutions	437:454	alkaline solutions	437:454	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	1	39	theme	pure	286:289	arg1	hydrogels					291:299	pure hydrogels	286:299	pure hydrogels	286:299	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	2	40	theme	pH	521:522	arg1	sensitivity					524:534	good pH sensitivity	516:534	good pH sensitivity	516:534	The results show that the composite hydrogel beads exhibit good pH sensitivity and controlled-release capacity, which could prolong the residence time of Cur in the gastrointestinal tract.					
30340010	2	41	theme	good	516:519	arg1	sensitivity					524:534	good pH sensitivity	516:534	good pH sensitivity	516:534	The results show that the composite hydrogel beads exhibit good pH sensitivity and controlled-release capacity, which could prolong the residence time of Cur in the gastrointestinal tract.					
30340010	1	42	theme	rapid	361:365	arg1	clearance					381:389	rapid physiological clearance	361:389	rapid physiological clearance	361:389	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	4	43	theme	light	1002:1006	arg1	degradation					1008:1018	light degradation	1002:1018	light degradation	1002:1018	The composite hydrogel beads protected the Cur from light degradation and can therefore prolong the antioxidant activity of Cur.					
30340010	3	44	theme	hydrogel	763:770	arg1	beads					772:776	Cur-loaded hydrogel beads	752:776	Cur-loaded hydrogel beads	752:776	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	1	45	theme	physiological	367:379	arg1	clearance					381:389	rapid physiological clearance	361:389	rapid physiological clearance	361:389	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	3	46	theme	beads	772:776	arg1	capacity					740:747	the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity	688:747	the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads	688:776	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	0	47	theme	sodium	71:76	arg1	beads					100:104	curcumin-loaded sodium alginate/ZnO hydrogel beads	55:104	curcumin-loaded sodium alginate/ZnO hydrogel beads	55:104	Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads.					
30340010	1	48	theme	curcumin	224:231	arg1	profile					213:219	the release profile	201:219	the release profile of curcumin (Cur)	201:237	In this study, we fabricated a series of novel sodium alginate/ZnO hydrogel beads to optimize the release profile of curcumin (Cur) and to avoid the burst release associated with pure hydrogels, which were used to mitigate the weaknesses of Cur, such as rapid physiological clearance and sensitivity to ultraviolet (UV) light and alkaline solutions.					
30340010	0	49	theme	curcumin-loaded	55:69	arg1	beads					100:104	curcumin-loaded sodium alginate/ZnO hydrogel beads	55:104	curcumin-loaded sodium alginate/ZnO hydrogel beads	55:104	Characterization, release, and antioxidant activity of curcumin-loaded sodium alginate/ZnO hydrogel beads.					
30340010	3	50	theme	encapsulated	895:906	arg1	Cur					908:910	the encapsulated Cur	891:910	the encapsulated Cur	891:910	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	3	51	theme	pure	824:827	arg1	Cur					829:831	pure Cur	824:831	pure Cur	824:831	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30340010	3	52	theme	Cur-loaded	752:761	arg1	beads					772:776	Cur-loaded hydrogel beads	752:776	Cur-loaded hydrogel beads	752:776	After exposure to UV irradiation for 6 h, the 1,1-Diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of Cur-loaded hydrogel beads was decreased by only 13.70%, whereas that of pure Cur decreased by 62.04% under the same conditions; therefore, the encapsulated Cur showed a higher antioxidant activity.					
30472472	4	0	theme	PVA/Chi	713:719	arg1	membranes					725:733	the PVA/Chi NFs membranes	709:733	the PVA/Chi NFs membranes	709:733	The adsorption data were also clarified that the PVA/Chi NFs membranes were exhibited high kinetic performances towards the both toxic ions at the optimum conditions.					
30472472	7	1	dep	confirmed	1356:1364	arg1	shown					1466:1470	shown	1466:1470	shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples	1466:1571	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	0	2	theme	ions	102:105	arg1	removal					107:113	selective lead(II) and cadmium(II) ions removal	67:113	selective lead(II) and cadmium(II) ions removal from wastewater	67:129	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	1	3	theme	cadmium	302:308	arg1	ions					319:322	lead (Pb(II)) and cadmium (Cd(II)) ions	284:322	lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity	284:352	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	7	4	theme	Cd	1232:1233	arg1	adsorptions					1244:1254	The Pb(II) and Cd(II) ions adsorptions	1217:1254	The Pb(II) and Cd(II) ions adsorptions	1217:1254	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	7	5	theme	efficient	1509:1517	arg1	removal					1519:1525	efficient removal	1509:1525	efficient removal of the selected toxic ions from waste samples	1509:1571	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	3	6	theme	ions	611:614	arg1	time					546:549	interaction time	534:549	interaction time	534:549	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	6	theme	ions	611:614	arg1	pH					579:580	solution pH	570:580	solution pH	570:580	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	6	theme	ions	611:614	arg1	effects					590:596	the effects	586:596	the effects of competing ions on Pb(II) and Cd(II) adsorption	586:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	6	theme	ions	611:614	arg1	ions					514:517	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	6	theme	ions	611:614	arg1	concentration					519:531	concentration	519:531	concentration	519:531	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	6	theme	ions	611:614	arg1	dosage					562:567	adsorbent dosage	552:567	adsorbent dosage	552:567	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	6	7	theme	adsorption	1084:1093	arg1	model					1105:1109	the Langmuir adsorption isotherms model	1071:1109	the Langmuir adsorption isotherms model	1071:1109	Similarly, the equilibrium data were well fitted with the Langmuir adsorption isotherms model, and the maximum adsorption capacity was 266.12 and 148.79 mg/g for Pb(II) and Cd(II) ions, respectively.					
30472472	1	8	theme	ions	319:322	arg1	adsorption					270:279	selective and high adsorption	251:279	adsorption	270:279	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	1	9	theme	solution	337:344	arg1	acidity					346:352	the solution acidity	333:352	the solution acidity	333:352	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	3	10	theme	several	460:466	arg1	ions					514:517	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	10	theme	several	460:466	arg1	parameters					481:490	several experimental parameters	460:490	several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	460:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	1	11	theme	nanofibers	136:145	arg1	membranes					147:155	The nanofibers membranes	132:155	The nanofibers membranes	132:155	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	7	12	theme	high	1370:1373	arg1	selectivity					1375:1385	the high selectivity	1366:1385	the high selectivity to Pb(II) and Cd(II) ions	1366:1411	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	2	13	theme	several	421:427	arg1	instrumentations					429:444	several instrumentations	421:444	several instrumentations	421:444	The PVA/Chi NFs membranes were characterized systematically using several instrumentations.					
30472472	3	14	from	effects	590:596	arg1	Cd					630:631	Cd(II)	630:635	Cd(II)	630:635	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	14	from	effects	590:596	arg1	Pb					619:620	Pb(II)	619:624	Pb(II)	619:624	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	6	15	theme	maximum	1120:1126	arg1	266.12					1152:1157	266.12	1152:1157	266.12	1152:1157	Similarly, the equilibrium data were well fitted with the Langmuir adsorption isotherms model, and the maximum adsorption capacity was 266.12 and 148.79 mg/g for Pb(II) and Cd(II) ions, respectively.					
30472472	6	15	theme	maximum	1120:1126	arg1	capacity					1139:1146	the maximum adsorption capacity	1116:1146	the maximum adsorption capacity	1116:1146	Similarly, the equilibrium data were well fitted with the Langmuir adsorption isotherms model, and the maximum adsorption capacity was 266.12 and 148.79 mg/g for Pb(II) and Cd(II) ions, respectively.					
30472472	4	16	theme	kinetic	755:761	arg1	performances					763:774	high kinetic performances	750:774	high kinetic performances towards the both toxic ions	750:802	The adsorption data were also clarified that the PVA/Chi NFs membranes were exhibited high kinetic performances towards the both toxic ions at the optimum conditions.					
30472472	2	17	theme	PVA/Chi	359:365	arg1	membranes					371:379	The PVA/Chi NFs membranes	355:379	The PVA/Chi NFs membranes	355:379	The PVA/Chi NFs membranes were characterized systematically using several instrumentations.					
30472472	7	18	theme	optimum	1420:1426	arg1	condition					1428:1436	the optimum condition	1416:1436	the optimum condition	1416:1436	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	2	19	theme	NFs	367:369	arg1	membranes					371:379	The PVA/Chi NFs membranes	355:379	The PVA/Chi NFs membranes	355:379	The PVA/Chi NFs membranes were characterized systematically using several instrumentations.					
30472472	3	20	dep	Pb	619:620	arg1	adsorption					637:646	adsorption	637:646	adsorption	637:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	21	theme	metal	508:512	arg1	time					546:549	interaction time	534:549	interaction time	534:549	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	21	theme	metal	508:512	arg1	dosage					562:567	adsorbent dosage	552:567	adsorbent dosage	552:567	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	21	theme	metal	508:512	arg1	pH					579:580	solution pH	570:580	solution pH	570:580	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	21	theme	metal	508:512	arg1	ions					514:517	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	21	theme	metal	508:512	arg1	concentration					519:531	concentration	519:531	concentration	519:531	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	7	22	theme	simulated	1304:1312	arg1	solution					1328:1335	simulated environmental solution	1304:1335	simulated environmental solution	1304:1335	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	4	23	dep	the	784:786	arg1	both					788:791	both	788:791	both	788:791	The adsorption data were also clarified that the PVA/Chi NFs membranes were exhibited high kinetic performances towards the both toxic ions at the optimum conditions.					
30472472	6	24	dep	Pb	1179:1180	arg1	ions					1197:1200	ions	1197:1200	ions	1197:1200	Similarly, the equilibrium data were well fitted with the Langmuir adsorption isotherms model, and the maximum adsorption capacity was 266.12 and 148.79 mg/g for Pb(II) and Cd(II) ions, respectively.					
30472472	3	25	from	concentration	519:531	arg1	Cd					630:631	Cd(II)	630:635	Cd(II)	630:635	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	25	from	concentration	519:531	arg1	Pb					619:620	Pb(II)	619:624	Pb(II)	619:624	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	0	26	theme	Composite	0:8	arg1	membranes					21:29	Composite nanofibers membranes	0:29	Composite nanofibers membranes of poly(vinyl alcohol)	0:52	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	0	27	from	wastewater	120:129	arg1	removal					107:113	selective lead(II) and cadmium(II) ions removal	67:113	selective lead(II) and cadmium(II) ions removal from wastewater	67:129	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	0	28	theme	poly	34:37	arg1	membranes					21:29	Composite nanofibers membranes	0:29	Composite nanofibers membranes of poly(vinyl alcohol)	0:52	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	7	29	dep	Pb	1221:1222	arg1	ions					1239:1242	ions	1239:1242	ions	1239:1242	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	7	29	dep	Pb	1221:1222	arg1	The					1217:1219	The	1217:1219	The	1217:1219	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	5	30	theme	different	874:882	arg1	models					893:898	different kinetics models	874:898	different kinetics models	874:898	The adsorption data were manipulated using different kinetics models, and it was confirmed that only pseudo-second-order model obeyed the adsorption kinetics for Pb(II) and Cd(II) ions.					
30472472	7	31	theme	toxic	1543:1547	arg1	ions					1549:1552	the selected toxic ions	1530:1552	the selected toxic ions	1530:1552	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	3	32	theme	interaction	534:544	arg1	time					546:549	interaction time	534:549	interaction time	534:549	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	32	theme	interaction	534:544	arg1	ions					514:517	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	0	33	theme	vinyl	39:43	arg1	poly					34:37	poly	34:37	poly(vinyl alcohol)	34:52	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	0	33	theme	vinyl	39:43	arg1	alcohol					45:51	vinyl alcohol	39:51	vinyl alcohol	39:51	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	1	34	theme	selective	251:259	arg1	adsorption					270:279	selective and high adsorption	251:279	adsorption	270:279	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	1	35	theme	high	265:268	arg1	adsorption					270:279	selective and high adsorption	251:279	adsorption	270:279	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	3	36	theme	solution	570:577	arg1	pH					579:580	solution pH	570:580	solution pH	570:580	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	36	theme	solution	570:577	arg1	ions					514:517	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	1	37	theme	poly	176:179	arg1	/chitosan					195:203	poly(vinyl alcohol)/chitosan	176:203	poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity	176:352	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	1	37	theme	poly	176:179	arg1	PVA/Chi					206:212	PVA/Chi	206:212	PVA/Chi	206:212	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	7	38	theme	waste	1559:1563	arg1	samples					1565:1571	waste samples	1559:1571	waste samples	1559:1571	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	8	39	theme	effective	1617:1625	arg1	materials					1641:1649	effective and promising materials	1617:1649	effective and promising materials for Pb(II) and Cd(II) ions	1617:1676	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	8	39	theme	effective	1617:1625	arg1	NFs					1592:1594	the PVA/Chi NFs	1580:1594	the PVA/Chi NFs	1580:1594	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	1	40	theme	vinyl	181:185	arg1	/chitosan					195:203	poly(vinyl alcohol)/chitosan	176:203	poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity	176:352	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	1	40	theme	vinyl	181:185	arg1	PVA/Chi					206:212	PVA/Chi	206:212	PVA/Chi	206:212	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	7	41	theme	possible	1493:1500	arg1	use					1502:1504	possible use	1493:1504	possible use in efficient removal of the selected toxic ions from waste samples	1493:1571	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	7	42	from	use	1502:1504	arg1	samples					1565:1571	waste samples	1559:1571	waste samples	1559:1571	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	7	42	from	use	1502:1504	arg1	removal					1519:1525	efficient removal	1509:1525	efficient removal of the selected toxic ions from waste samples	1509:1571	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	6	43	theme	isotherms	1095:1103	arg1	model					1105:1109	the Langmuir adsorption isotherms model	1071:1109	the Langmuir adsorption isotherms model	1071:1109	Similarly, the equilibrium data were well fitted with the Langmuir adsorption isotherms model, and the maximum adsorption capacity was 266.12 and 148.79 mg/g for Pb(II) and Cd(II) ions, respectively.					
30472472	4	44	theme	NFs	721:723	arg1	membranes					725:733	the PVA/Chi NFs membranes	709:733	the PVA/Chi NFs membranes	709:733	The adsorption data were also clarified that the PVA/Chi NFs membranes were exhibited high kinetic performances towards the both toxic ions at the optimum conditions.					
30472472	3	45	from	time	546:549	arg1	Cd					630:631	Cd(II)	630:635	Cd(II)	630:635	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	45	from	time	546:549	arg1	Pb					619:620	Pb(II)	619:624	Pb(II)	619:624	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	6	46	theme	Langmuir	1075:1082	arg1	adsorption					1084:1093	the Langmuir adsorption	1071:1093	the Langmuir adsorption isotherms model	1071:1109	Similarly, the equilibrium data were well fitted with the Langmuir adsorption isotherms model, and the maximum adsorption capacity was 266.12 and 148.79 mg/g for Pb(II) and Cd(II) ions, respectively.					
30472472	7	47	theme	Pb	1221:1222	arg1	adsorptions					1244:1254	The Pb(II) and Cd(II) ions adsorptions	1217:1254	The Pb(II) and Cd(II) ions adsorptions	1217:1254	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	8	48	dep	Pb	1655:1656	arg1	ions					1673:1676	ions	1673:1676	ions	1673:1676	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	3	49	theme	competing	601:609	arg1	ions					611:614	competing ions	601:614	competing ions	601:614	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	8	50	theme	PVA/Chi	1584:1590	arg1	NFs					1592:1594	the PVA/Chi NFs	1580:1594	the PVA/Chi NFs	1580:1594	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	8	50	theme	PVA/Chi	1584:1590	arg1	materials					1641:1649	effective and promising materials	1617:1649	effective and promising materials for Pb(II) and Cd(II) ions	1617:1676	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	7	51	theme	nanofibers	1446:1455	arg1	membrane					1457:1464	the nanofibers membrane	1442:1464	the nanofibers membrane	1442:1464	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	0	52	theme	cadmium	90:96	arg1	ions					102:105	cadmium(II) ions	90:105	cadmium(II) ions	90:105	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	3	53	from	pH	579:580	arg1	Cd					630:631	Cd(II)	630:635	Cd(II)	630:635	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	53	from	pH	579:580	arg1	Pb					619:620	Pb(II)	619:624	Pb(II)	619:624	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	54	dep	ions	514:517	arg1	time					546:549	interaction time	534:549	interaction time	534:549	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	54	dep	ions	514:517	arg1	pH					579:580	solution pH	570:580	solution pH	570:580	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	54	dep	ions	514:517	arg1	effects					590:596	the effects	586:596	the effects of competing ions on Pb(II) and Cd(II) adsorption	586:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	54	dep	ions	514:517	arg1	ions					514:517	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	54	dep	ions	514:517	arg1	concentration					519:531	concentration	519:531	concentration	519:531	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	54	dep	ions	514:517	arg1	dosage					562:567	adsorbent dosage	552:567	adsorbent dosage	552:567	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	4	55	theme	the	784:786	arg1	ions					799:802	the both toxic ions	784:802	ions	799:802	The adsorption data were also clarified that the PVA/Chi NFs membranes were exhibited high kinetic performances towards the both toxic ions at the optimum conditions.					
30472472	6	56	theme	adsorption	1128:1137	arg1	266.12					1152:1157	266.12	1152:1157	266.12	1152:1157	Similarly, the equilibrium data were well fitted with the Langmuir adsorption isotherms model, and the maximum adsorption capacity was 266.12 and 148.79 mg/g for Pb(II) and Cd(II) ions, respectively.					
30472472	6	56	theme	adsorption	1128:1137	arg1	capacity					1139:1146	the maximum adsorption capacity	1116:1146	the maximum adsorption capacity	1116:1146	Similarly, the equilibrium data were well fitted with the Langmuir adsorption isotherms model, and the maximum adsorption capacity was 266.12 and 148.79 mg/g for Pb(II) and Cd(II) ions, respectively.					
30472472	5	57	theme	pseudo-second-order	932:950	arg1	model					952:956	only pseudo-second-order model	927:956	only pseudo-second-order model	927:956	The adsorption data were manipulated using different kinetics models, and it was confirmed that only pseudo-second-order model obeyed the adsorption kinetics for Pb(II) and Cd(II) ions.					
30472472	3	58	theme	experimental	468:479	arg1	ions					514:517	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	58	theme	experimental	468:479	arg1	parameters					481:490	several experimental parameters	460:490	several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	460:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	7	59	with	selectivity	1287:1297	arg1	solution					1328:1335	simulated environmental solution	1304:1335	simulated environmental solution	1304:1335	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	6	60	theme	equilibrium	1032:1042	arg1	data					1044:1047	the equilibrium data	1028:1047	the equilibrium data	1028:1047	Similarly, the equilibrium data were well fitted with the Langmuir adsorption isotherms model, and the maximum adsorption capacity was 266.12 and 148.79 mg/g for Pb(II) and Cd(II) ions, respectively.					
30472472	8	61	theme	promising	1631:1639	arg1	materials					1641:1649	effective and promising materials	1617:1649	effective and promising materials for Pb(II) and Cd(II) ions	1617:1676	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	8	61	theme	promising	1631:1639	arg1	NFs					1592:1594	the PVA/Chi NFs	1580:1594	the PVA/Chi NFs	1580:1594	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	4	62	theme	high	750:753	arg1	performances					763:774	high kinetic performances	750:774	high kinetic performances towards the both toxic ions	750:802	The adsorption data were also clarified that the PVA/Chi NFs membranes were exhibited high kinetic performances towards the both toxic ions at the optimum conditions.					
30472472	7	63	dep	Pb	1390:1391	arg1	ions					1408:1411	ions	1408:1411	ions	1408:1411	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	3	64	theme	initial	500:506	arg1	time					546:549	interaction time	534:549	interaction time	534:549	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	64	theme	initial	500:506	arg1	dosage					562:567	adsorbent dosage	552:567	adsorbent dosage	552:567	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	64	theme	initial	500:506	arg1	pH					579:580	solution pH	570:580	solution pH	570:580	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	64	theme	initial	500:506	arg1	ions					514:517	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	64	theme	initial	500:506	arg1	concentration					519:531	concentration	519:531	concentration	519:531	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	7	65	from	samples	1565:1571	arg1	removal					1519:1525	efficient removal	1509:1525	efficient removal of the selected toxic ions from waste samples	1509:1571	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	7	65	from	samples	1565:1571	arg1	use					1502:1504	possible use	1493:1504	possible use in efficient removal of the selected toxic ions from waste samples	1493:1571	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	4	66	theme	optimum	811:817	arg1	conditions					819:828	the optimum conditions	807:828	the optimum conditions	807:828	The adsorption data were also clarified that the PVA/Chi NFs membranes were exhibited high kinetic performances towards the both toxic ions at the optimum conditions.					
30472472	0	67	theme	lead	77:80	arg1	removal					107:113	selective lead(II) and cadmium(II) ions removal	67:113	selective lead(II) and cadmium(II) ions removal from wastewater	67:129	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	0	68	theme	selective	67:75	arg1	removal					107:113	selective lead(II) and cadmium(II) ions removal	67:113	selective lead(II) and cadmium(II) ions removal from wastewater	67:129	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	1	69	theme	lead	284:287	arg1	ions					319:322	lead (Pb(II)) and cadmium (Cd(II)) ions	284:322	lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity	284:352	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	8	70	from	wastewaters	1683:1693	arg1	materials					1641:1649	effective and promising materials	1617:1649	effective and promising materials for Pb(II) and Cd(II) ions	1617:1676	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	8	70	from	wastewaters	1683:1693	arg1	NFs					1592:1594	the PVA/Chi NFs	1580:1594	the PVA/Chi NFs	1580:1594	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	0	71	theme	nanofibers	10:19	arg1	membranes					21:29	Composite nanofibers membranes	0:29	Composite nanofibers membranes of poly(vinyl alcohol)	0:52	Composite nanofibers membranes of poly(vinyl alcohol)/chitosan for selective lead(II) and cadmium(II) ions removal from wastewater.					
30472472	5	72	theme	adsorption	969:978	arg1	kinetics					980:987	the adsorption kinetics	965:987	the adsorption kinetics for Pb(II) and Cd(II) ions	965:1014	The adsorption data were manipulated using different kinetics models, and it was confirmed that only pseudo-second-order model obeyed the adsorption kinetics for Pb(II) and Cd(II) ions.					
30472472	5	73	dep	Pb	993:994	arg1	ions					1011:1014	ions	1011:1014	ions	1011:1014	The adsorption data were manipulated using different kinetics models, and it was confirmed that only pseudo-second-order model obeyed the adsorption kinetics for Pb(II) and Cd(II) ions.					
30472472	1	74	theme	electro-spun	224:235	arg1	technique					237:245	an electro-spun technique	221:245	an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity	221:352	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	8	75	theme	high	1700:1703	arg1	efficiency					1705:1714	high efficiency	1700:1714	high efficiency	1700:1714	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	5	76	theme	kinetics	884:891	arg1	models					893:898	different kinetics models	874:898	different kinetics models	874:898	The adsorption data were manipulated using different kinetics models, and it was confirmed that only pseudo-second-order model obeyed the adsorption kinetics for Pb(II) and Cd(II) ions.					
30472472	7	77	theme	ions	1549:1552	arg1	removal					1519:1525	efficient removal	1509:1525	efficient removal of the selected toxic ions from waste samples	1509:1571	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	4	78	theme	adsorption	668:677	arg1	data					679:682	The adsorption data	664:682	The adsorption data	664:682	The adsorption data were also clarified that the PVA/Chi NFs membranes were exhibited high kinetic performances towards the both toxic ions at the optimum conditions.					
30472472	5	79	theme	adsorption	835:844	arg1	data					846:849	The adsorption data	831:849	The adsorption data	831:849	The adsorption data were manipulated using different kinetics models, and it was confirmed that only pseudo-second-order model obeyed the adsorption kinetics for Pb(II) and Cd(II) ions.					
30472472	7	80	theme	selected	1534:1541	arg1	ions					1549:1552	the selected toxic ions	1530:1552	the selected toxic ions	1530:1552	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	3	81	theme	adsorbent	552:560	arg1	dosage					562:567	adsorbent dosage	552:567	adsorbent dosage	552:567	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	81	theme	adsorbent	552:560	arg1	ions					514:517	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption	500:646	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	7	82	theme	environmental	1314:1326	arg1	solution					1328:1335	simulated environmental solution	1304:1335	simulated environmental solution	1304:1335	The Pb(II) and Cd(II) ions adsorptions were also measured to know the selectivity with simulated environmental solution, and the data were confirmed the high selectivity to Pb(II) and Cd(II) ions at the optimum condition and the nanofibers membrane shown the potentiality for possible use in efficient removal of the selected toxic ions from waste samples.					
30472472	3	83	from	dosage	562:567	arg1	Cd					630:631	Cd(II)	630:635	Cd(II)	630:635	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	3	83	from	dosage	562:567	arg1	Pb					619:620	Pb(II)	619:624	Pb(II)	619:624	In addition, several experimental parameters such as initial metal ions concentration, interaction time, adsorbent dosage, solution pH and the effects of competing ions on Pb(II) and Cd(II) adsorption were evaluated.					
30472472	8	84	with	wastewaters	1683:1693	arg1	efficiency					1705:1714	high efficiency	1700:1714	high efficiency	1700:1714	Thus, the PVA/Chi NFs are considered to be effective and promising materials for Pb(II) and Cd(II) ions from wastewaters with high efficiency.					
30472472	1	85	theme	alcohol	187:193	arg1	/chitosan					195:203	poly(vinyl alcohol)/chitosan	176:203	poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity	176:352	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
30472472	1	85	theme	alcohol	187:193	arg1	PVA/Chi					206:212	PVA/Chi	206:212	PVA/Chi	206:212	The nanofibers membranes were fabricated by poly(vinyl alcohol)/chitosan (PVA/Chi) using an electro-spun technique for selective and high adsorption of lead (Pb(II)) and cadmium (Cd(II)) ions based on the solution acidity.					
29885393	8	0	theme	due	1065:1067	arg1	scaffold					1056:1063	the scaffold	1052:1063	the scaffold due to the higher stiffness contributed by nHAp	1052:1111	Water uptake and degradation ability significantly reduced with the increase of nHAp in the scaffold due to the higher stiffness contributed by nHAp.					
29885393	12	1	dep	biocompatibility	1612:1627	arg1	enhance					1604:1610	enhance	1604:1610	enhance	1604:1610	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	2	2	theme	engineering	396:406	arg1	application					408:418	tissue engineering application	389:418	tissue engineering application	389:418	The physico-chemical properties of the scaffolds were evaluated in order to assess its suitability for tissue engineering application.					
29885393	11	3	theme	scaffolds	1389:1397	arg1	Cytotoxicity					1365:1376	Cytotoxicity	1365:1376	Cytotoxicity of the all scaffolds	1365:1397	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	12	4	dep	properties	1589:1598	arg1	These					1566:1570	These	1566:1570	These	1566:1570	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	10	5	theme	crystallinity	1295:1307	arg1	increase					1283:1290	the increase	1279:1290	the increase of crystallinity in the composites due to incorporation of nHAp and ADA	1279:1362	XRD analysis of the scaffolds shows the increase of crystallinity in the composites due to incorporation of nHAp and ADA.					
29885393	2	6	theme	tissue	389:394	arg1	application					408:418	tissue engineering application	389:418	tissue engineering application	389:418	The physico-chemical properties of the scaffolds were evaluated in order to assess its suitability for tissue engineering application.					
29885393	12	7	theme	promising	1719:1727	arg1	candidate					1729:1737	a promising candidate	1717:1737	a promising candidate for biomedical application	1717:1764	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	11	8	theme	kidney	1423:1428	arg1	cells					1441:1445	normal kidney epithelial cells	1416:1445	normal kidney epithelial cells (Vero cells)	1416:1458	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	11	8	theme	kidney	1423:1428	arg1	cells					1453:1457	Vero cells	1448:1457	Vero cells	1448:1457	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	0	9	link	cross-linked	27:38	arg1	gelatin/nano-hydroxyapatite					40:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite	0:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite	0:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite bioscaffolds for soft tissue regeneration.					
29885393	6	10	dep	ratio	791:795	arg1	to					786:787	to	786:787	to	786:787	Ca to P ratio of nHAp is 1.51 as observed by EDX confirmed the suitability of the scaffold for biomedical application.					
29885393	8	11	theme	degradation	981:991	arg1	ability					993:999	degradation ability	981:999	degradation ability	981:999	Water uptake and degradation ability significantly reduced with the increase of nHAp in the scaffold due to the higher stiffness contributed by nHAp.					
29885393	3	12	theme	base	598:601	arg1	formation					603:611	Schiff's base formation	589:611	Schiff's base formation	589:611	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	2	13	theme	scaffolds	325:333	arg1	properties					307:316	The physico-chemical properties	286:316	The physico-chemical properties of the scaffolds	286:333	The physico-chemical properties of the scaffolds were evaluated in order to assess its suitability for tissue engineering application.					
29885393	11	14	theme	Vero	1448:1451	arg1	cells					1441:1445	normal kidney epithelial cells	1416:1445	normal kidney epithelial cells (Vero cells)	1416:1458	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	11	14	theme	Vero	1448:1451	arg1	cells					1453:1457	Vero cells	1448:1457	Vero cells	1448:1457	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	3	15	theme	gelatin	537:543	arg1	group					568:572	available aldehyde group	549:572	available aldehyde group of ADA	549:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	15	theme	gelatin	537:543	arg1	ADA					577:579	ADA	577:579	ADA	577:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	15	theme	gelatin	537:543	arg1	gelatin					537:543	gelatin	537:543	gelatin	537:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	15	theme	gelatin	537:543	arg1	group					528:532	free amino group	517:532	free amino group of gelatin	517:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	9	16	from	proliferation	1203:1215	arg1	scaffolds					1232:1240	the scaffolds	1228:1240	the scaffolds	1228:1240	SEM analysis revealed that the pore size and porosity of the scaffolds declines with the proliferation of nHAp in the scaffolds.					
29885393	12	17	theme	tissue	1684:1689	arg1	regeneration					1691:1702	soft tissue regeneration	1679:1702	soft tissue regeneration	1679:1702	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	9	18	theme	nHAp	1220:1223	arg1	proliferation					1203:1215	the proliferation	1199:1215	the proliferation of nHAp in the scaffolds	1199:1240	SEM analysis revealed that the pore size and porosity of the scaffolds declines with the proliferation of nHAp in the scaffolds.					
29885393	12	19	theme	tunable	1572:1578	arg1	properties					1589:1598	tunable physical properties	1572:1598	tunable physical properties	1572:1598	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	12	20	theme	soft	1679:1682	arg1	regeneration					1691:1702	soft tissue regeneration	1679:1702	soft tissue regeneration	1679:1702	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	1	21	theme	gelatin	206:212	arg1	bioscaffold					247:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold	158:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	158:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	4	22	theme	waste	640:644	arg1	egg-shells					646:655	waste egg-shells	640:655	waste egg-shells	640:655	nHAp was synthesized from waste egg-shells by wet chemical method.					
29885393	8	23	theme	higher	1076:1081	arg1	stiffness					1083:1091	the higher stiffness	1072:1091	the higher stiffness contributed by nHAp	1072:1111	Water uptake and degradation ability significantly reduced with the increase of nHAp in the scaffold due to the higher stiffness contributed by nHAp.					
29885393	8	24	theme	nHAp	1044:1047	arg1	increase					1032:1039	the increase	1028:1039	the increase of nHAp	1028:1047	Water uptake and degradation ability significantly reduced with the increase of nHAp in the scaffold due to the higher stiffness contributed by nHAp.					
29885393	1	25	theme	present	115:121	arg1	study					123:127	The present study	111:127	The present study	111:127	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	4	26	theme	wet	660:662	arg1	method					673:678	wet chemical method	660:678	wet chemical method	660:678	nHAp was synthesized from waste egg-shells by wet chemical method.					
29885393	0	27	theme	alginate-di-aldehyde	6:25	arg1	gelatin/nano-hydroxyapatite					40:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite	0:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite	0:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite bioscaffolds for soft tissue regeneration.					
29885393	3	28	theme	ADA	577:579	arg1	group					568:572	available aldehyde group	549:572	available aldehyde group of ADA	549:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	28	theme	ADA	577:579	arg1	ADA					577:579	ADA	577:579	ADA	577:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	28	theme	ADA	577:579	arg1	gelatin					537:543	gelatin	537:543	gelatin	537:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	28	theme	ADA	577:579	arg1	group					528:532	free amino group	517:532	free amino group of gelatin	517:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	5	29	theme	synthesized	685:695	arg1	HAp					697:699	The synthesized HAp	681:699	The synthesized HAp	681:699	The synthesized HAp was found crystalline and nanosize (~45 nm) by XRD and TEM analysis respectively.					
29885393	1	30	theme	GEL	215:217	arg1	bioscaffold					247:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold	158:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	158:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	0	31	theme	Novel	0:4	arg1	gelatin/nano-hydroxyapatite					40:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite	0:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite	0:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite bioscaffolds for soft tissue regeneration.					
29885393	12	32	theme	biomedical	1743:1752	arg1	application					1754:1764	biomedical application	1743:1764	biomedical application	1743:1764	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	7	33	theme	scaffolds	953:961	arg1	stability					940:948	thermal stability	932:948	thermal stability of scaffolds	932:961	The crosslinked ADA increases thermal stability of scaffolds.					
29885393	10	34	theme	nHAp	1351:1354	arg1	incorporation					1334:1346	incorporation	1334:1346	incorporation of nHAp and ADA	1334:1362	XRD analysis of the scaffolds shows the increase of crystallinity in the composites due to incorporation of nHAp and ADA.					
29885393	6	35	theme	nHAp	800:803	arg1	ratio					791:795	Ca to P ratio	783:795	Ca to P ratio of nHAp	783:803	Ca to P ratio of nHAp is 1.51 as observed by EDX confirmed the suitability of the scaffold for biomedical application.					
29885393	10	36	from	increase	1283:1290	arg1	composites					1316:1325	the composites	1312:1325	the composites due to incorporation of nHAp and ADA	1312:1362	XRD analysis of the scaffolds shows the increase of crystallinity in the composites due to incorporation of nHAp and ADA.					
29885393	1	37	theme	/nano-hydroxyapatite	219:238	arg1	bioscaffold					247:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold	158:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	158:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	0	38	theme	cross-linked	27:38	arg1	gelatin/nano-hydroxyapatite					40:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite	0:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite	0:66	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite bioscaffolds for soft tissue regeneration.					
29885393	12	39	theme	scaffold	1641:1648	arg1	properties					1589:1598	tunable physical properties	1572:1598	tunable physical properties	1572:1598	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	12	39	theme	scaffold	1641:1648	arg1	biocompatibility					1612:1627	enhance biocompatibility	1604:1627	enhance biocompatibility	1604:1627	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	1	40	link	cross-linked	193:204	arg1	bioscaffold					247:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold	158:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	158:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	11	41	theme	epithelial	1430:1439	arg1	cells					1441:1445	normal kidney epithelial cells	1416:1445	normal kidney epithelial cells (Vero cells)	1416:1458	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	11	41	theme	epithelial	1430:1439	arg1	cells					1453:1457	Vero cells	1448:1457	Vero cells	1448:1457	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	1	42	theme	cross-linked	193:204	arg1	bioscaffold					247:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold	158:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	158:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	10	43	theme	due	1327:1329	arg1	composites					1316:1325	the composites	1312:1325	the composites due to incorporation of nHAp and ADA	1312:1362	XRD analysis of the scaffolds shows the increase of crystallinity in the composites due to incorporation of nHAp and ADA.					
29885393	3	44	theme	aldehyde	559:566	arg1	group					568:572	available aldehyde group	549:572	available aldehyde group of ADA	549:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	44	theme	aldehyde	559:566	arg1	gelatin					537:543	gelatin	537:543	gelatin	537:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	44	theme	aldehyde	559:566	arg1	ADA					577:579	ADA	577:579	ADA	577:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	12	45	theme	prepared	1632:1639	arg1	scaffold					1641:1648	prepared scaffold	1632:1648	prepared scaffold	1632:1648	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	6	46	theme	biomedical	878:887	arg1	application					889:899	biomedical application	878:899	biomedical application	878:899	Ca to P ratio of nHAp is 1.51 as observed by EDX confirmed the suitability of the scaffold for biomedical application.					
29885393	9	47	theme	pore	1145:1148	arg1	size					1150:1153	pore size	1145:1153	pore size	1145:1153	SEM analysis revealed that the pore size and porosity of the scaffolds declines with the proliferation of nHAp in the scaffolds.					
29885393	1	48	theme	nHAp	241:244	arg1	bioscaffold					247:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold	158:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	158:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	3	49	theme	alginate	466:473	arg1	oxidation					453:461	periodate oxidation	443:461	periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation	443:611	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	5	50	theme	TEM	756:758	arg1	analysis					760:767	TEM analysis	756:767	TEM analysis	756:767	The synthesized HAp was found crystalline and nanosize (~45 nm) by XRD and TEM analysis respectively.					
29885393	7	51	link	crosslinked	906:916	arg1	ADA					918:920	The crosslinked ADA	902:920	The crosslinked ADA	902:920	The crosslinked ADA increases thermal stability of scaffolds.					
29885393	11	52	theme	normal	1416:1421	arg1	cells					1441:1445	normal kidney epithelial cells	1416:1445	normal kidney epithelial cells (Vero cells)	1416:1458	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	11	52	theme	normal	1416:1421	arg1	cells					1453:1457	Vero cells	1448:1457	Vero cells	1448:1457	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	10	53	theme	ADA	1360:1362	arg1	incorporation					1334:1346	incorporation	1334:1346	incorporation of nHAp and ADA	1334:1362	XRD analysis of the scaffolds shows the increase of crystallinity in the composites due to incorporation of nHAp and ADA.					
29885393	3	54	theme	free	517:520	arg1	ADA					577:579	ADA	577:579	ADA	577:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	54	theme	free	517:520	arg1	gelatin					537:543	gelatin	537:543	gelatin	537:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	54	theme	free	517:520	arg1	group					528:532	free amino group	517:532	free amino group of gelatin	517:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	0	55	theme	tissue	90:95	arg1	regeneration					97:108	soft tissue regeneration	85:108	soft tissue regeneration	85:108	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite bioscaffolds for soft tissue regeneration.					
29885393	12	56	theme	physical	1580:1587	arg1	properties					1589:1598	tunable physical properties	1572:1598	tunable physical properties	1572:1598	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	6	57	theme	scaffold	865:872	arg1	suitability					846:856	the suitability	842:856	the suitability of the scaffold for biomedical application	842:899	Ca to P ratio of nHAp is 1.51 as observed by EDX confirmed the suitability of the scaffold for biomedical application.					
29885393	10	58	theme	XRD	1243:1245	arg1	analysis					1247:1254	XRD analysis	1243:1254	XRD analysis of the scaffolds	1243:1271	XRD analysis of the scaffolds shows the increase of crystallinity in the composites due to incorporation of nHAp and ADA.					
29885393	12	59	from	application	1664:1674	arg1	regeneration					1691:1702	soft tissue regeneration	1679:1702	soft tissue regeneration	1679:1702	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	0	60	theme	soft	85:88	arg1	regeneration					97:108	soft tissue regeneration	85:108	soft tissue regeneration	85:108	Novel alginate-di-aldehyde cross-linked gelatin/nano-hydroxyapatite bioscaffolds for soft tissue regeneration.					
29885393	6	61	theme	P	789:789	arg1	ratio					791:795	Ca to P ratio	783:795	Ca to P ratio of nHAp	783:803	Ca to P ratio of nHAp is 1.51 as observed by EDX confirmed the suitability of the scaffold for biomedical application.					
29885393	3	62	theme	available	549:557	arg1	group					568:572	available aldehyde group	549:572	available aldehyde group of ADA	549:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	62	theme	available	549:557	arg1	gelatin					537:543	gelatin	537:543	gelatin	537:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	62	theme	available	549:557	arg1	ADA					577:579	ADA	577:579	ADA	577:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	12	63	dep	application	1664:1674	arg1	advance					1656:1662	advance	1656:1662	advance	1656:1662	These tunable physical properties and enhance biocompatibility of prepared scaffold offer advance application in soft tissue regeneration and could be a promising candidate for biomedical application.					
29885393	8	64	theme	Water	964:968	arg1	uptake					970:975	Water uptake	964:975	Water uptake	964:975	Water uptake and degradation ability significantly reduced with the increase of nHAp in the scaffold due to the higher stiffness contributed by nHAp.					
29885393	1	65	theme	novel	160:164	arg1	bioscaffold					247:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold	158:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	158:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	1	66	theme	bioscaffold	247:257	arg1	fabrication					143:153	the fabrication	139:153	the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	139:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	9	67	theme	scaffolds	1175:1183	arg1	porosity					1159:1166	porosity	1159:1166	porosity	1159:1166	SEM analysis revealed that the pore size and porosity of the scaffolds declines with the proliferation of nHAp in the scaffolds.					
29885393	9	67	theme	scaffolds	1175:1183	arg1	size					1150:1153	pore size	1145:1153	pore size	1145:1153	SEM analysis revealed that the pore size and porosity of the scaffolds declines with the proliferation of nHAp in the scaffolds.					
29885393	2	68	theme	physico-chemical	290:305	arg1	properties					307:316	The physico-chemical properties	286:316	The physico-chemical properties of the scaffolds	286:333	The physico-chemical properties of the scaffolds were evaluated in order to assess its suitability for tissue engineering application.					
29885393	6	69	theme	Ca	783:784	arg1	ratio					791:795	Ca to P ratio	783:795	Ca to P ratio of nHAp	783:803	Ca to P ratio of nHAp is 1.51 as observed by EDX confirmed the suitability of the scaffold for biomedical application.					
29885393	1	70	theme	alginate-di-aldehyde	166:185	arg1	bioscaffold					247:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold	158:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	158:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	9	71	theme	SEM	1114:1116	arg1	analysis					1118:1125	SEM analysis	1114:1125	SEM analysis	1114:1125	SEM analysis revealed that the pore size and porosity of the scaffolds declines with the proliferation of nHAp in the scaffolds.					
29885393	10	72	theme	scaffolds	1263:1271	arg1	analysis					1247:1254	XRD analysis	1243:1254	XRD analysis of the scaffolds	1243:1271	XRD analysis of the scaffolds shows the increase of crystallinity in the composites due to incorporation of nHAp and ADA.					
29885393	11	73	theme	scaffolds	1510:1518	arg1	non-toxicity					1490:1501	the non-toxicity	1486:1501	the non-toxicity	1486:1501	Cytotoxicity of the all scaffolds were examined by normal kidney epithelial cells (Vero cells) and the results confirmed the non-toxicity of the scaffolds, which proved it is extremely cytocompatible.					
29885393	3	74	theme	amino	522:526	arg1	ADA					577:579	ADA	577:579	ADA	577:579	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	74	theme	amino	522:526	arg1	gelatin					537:543	gelatin	537:543	gelatin	537:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	3	74	theme	amino	522:526	arg1	group					528:532	free amino group	517:532	free amino group of gelatin	517:543	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	7	75	theme	thermal	932:938	arg1	stability					940:948	thermal stability	932:948	thermal stability of scaffolds	932:961	The crosslinked ADA increases thermal stability of scaffolds.					
29885393	1	76	theme	lyophilization	262:275	arg1	process					277:283	lyophilization process	262:283	lyophilization process	262:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	3	77	theme	periodate	443:451	arg1	oxidation					453:461	periodate oxidation	443:461	periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation	443:611	ADA was prepared from periodate oxidation of alginate which facilitate the crosslinking between free amino group of gelatin and available aldehyde group of ADA through Schiff's base formation.					
29885393	1	78	theme	ADA	188:190	arg1	bioscaffold					247:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold	158:257	a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process	158:283	The present study describes the fabrication of a novel alginate-di-aldehyde (ADA) cross-linked gelatin (GEL)/nano-hydroxyapatite (nHAp) bioscaffold by lyophilization process.					
29885393	7	79	theme	crosslinked	906:916	arg1	ADA					918:920	The crosslinked ADA	902:920	The crosslinked ADA	902:920	The crosslinked ADA increases thermal stability of scaffolds.					
29885393	4	80	theme	chemical	664:671	arg1	method					673:678	wet chemical method	660:678	wet chemical method	660:678	nHAp was synthesized from waste egg-shells by wet chemical method.					
29524383	3	0	theme	free	455:458	arg1	media					480:484	calcium (Ca2+) free or -containing base media	440:484	calcium (Ca2+) free or -containing base media	440:484	In both protocols, calcium (Ca2+) free or -containing base media were tested.					
29524383	12	1	theme	Ca2+	1615:1618	arg1	concentration					1620:1632	Ca2+ concentration	1615:1632	Ca2+ concentration	1615:1632	In conclusion, the most effective protocol for bovine oocytes cryopreservation combines EG and DMSO, independently of Ca2+ concentration in the media.					
29524383	0	2	theme	cryoprotectants	75:89	arg1	cryosurvival					40:51	cryosurvival	40:51	cryosurvival	40:51	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	2	theme	cryoprotectants	75:89	arg1	permeability					23:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	2	theme	cryoprotectants	75:89	arg1	Effects					54:60	Effects	54:60	Effects of different cryoprotectants and calcium in the vitrification media	54:128	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	8	3	theme	cleavage	991:998	arg1	rates					1000:1004	higher cleavage rates	984:1004	higher cleavage rates	984:1004	After warming, higher cleavage rates were obtained in EGDMSO + Ca2+ compared to the PrOH without Ca2+ protocol (p = 0.02).					
29524383	7	4	theme	EGDMSO	938:943	arg1	p = 0.0005					956:965	p = 0.0005	956:965	p = 0.0005	956:965	Independently of Ca2+ concentration in the vitrification media, the development rates were higher in oocytes vitrified with EGDMSO protocols (p = 0.0005).					
29524383	7	4	theme	EGDMSO	938:943	arg1	protocols					945:953	EGDMSO protocols	938:953	EGDMSO protocols (p = 0.0005)	938:966	Independently of Ca2+ concentration in the vitrification media, the development rates were higher in oocytes vitrified with EGDMSO protocols (p = 0.0005).					
29524383	14	5	theme	CPAs	1764:1767	arg1	type					1756:1759	The type	1752:1759	The type of CPAs and Ca2+	1752:1776	The type of CPAs and Ca2+ interfered differentially on oocyte pathways to functionality, and this should be considered when choosing a cryopreservation protocol.					
29524383	10	6	theme	zona	1214:1217	arg1	hardening					1219:1227	An enhanced premature zona hardening	1192:1227	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1192:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	6	7	theme	membrane	750:757	arg1	permeability					759:770	membrane permeability	750:770	membrane permeability	750:770	Finally, fatty acid composition and membrane permeability of oocytes exposed to CPAs were analyzed.					
29524383	9	8	theme	cleavage	1145:1152	arg1	rates					1154:1158	lower cleavage rates	1139:1158	lower cleavage rates	1139:1158	Oocytes exposed to PrOH without Ca2+ presented lower cleavage rates compared to control (p = 0.04).					
29524383	10	9	theme	premature	1204:1212	arg1	hardening					1219:1227	An enhanced premature zona hardening	1192:1227	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1192:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	0	10	theme	calcium	95:101	arg1	cryosurvival					40:51	cryosurvival	40:51	cryosurvival	40:51	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	10	theme	calcium	95:101	arg1	permeability					23:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	10	theme	calcium	95:101	arg1	Effects					54:60	Effects	54:60	Effects of different cryoprotectants and calcium in the vitrification media	54:128	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	11	from	Effects	54:60	arg1	media					124:128	the vitrification media	106:128	the vitrification media	106:128	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	10	12	theme	acids	1295:1299	arg1	concentrations					1267:1280	lower concentrations	1261:1280	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1192:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	12	theme	acids	1295:1299	arg1	hardening					1219:1227	An enhanced premature zona hardening	1192:1227	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1192:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	6	13	theme	oocytes	775:781	arg1	composition					734:744	fatty acid composition	723:744	fatty acid composition	723:744	Finally, fatty acid composition and membrane permeability of oocytes exposed to CPAs were analyzed.					
29524383	6	13	theme	oocytes	775:781	arg1	permeability					759:770	membrane permeability	750:770	membrane permeability	750:770	Finally, fatty acid composition and membrane permeability of oocytes exposed to CPAs were analyzed.					
29524383	2	14	theme	ethylene	298:305	arg1	EG					315:316	EG	315:316	EG	315:316	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	14	theme	ethylene	298:305	arg1	glycol					307:312	either ethylene glycol	291:312	either ethylene glycol (EG)	291:317	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	7	15	theme	Ca2+	831:834	arg1	concentration					836:848	Ca2+ concentration	831:848	Ca2+ concentration in the vitrification media	831:875	Independently of Ca2+ concentration in the vitrification media, the development rates were higher in oocytes vitrified with EGDMSO protocols (p = 0.0005).					
29524383	2	16	theme	dimethyl	320:327	arg1	EGDMSO					359:364	EGDMSO	359:364	EGDMSO	359:364	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	16	theme	dimethyl	320:327	arg1	DMSO					340:343	DMSO	340:343	DMSO	340:343	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	16	theme	dimethyl	320:327	arg1	sulfoxide					329:337	dimethyl sulfoxide	320:337	dimethyl sulfoxide (DMSO)	320:344	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	16	theme	dimethyl	320:327	arg1	cryoprotectants					256:270	different cryoprotectants	246:270	different cryoprotectants (CPAs)	246:277	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	0	17	theme	vitrification	110:122	arg1	media					124:128	the vitrification media	106:128	the vitrification media	106:128	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	10	18	theme	oocyte	1333:1338	arg1	complexes					1340:1348	cumulus oocyte complexes	1325:1348	cumulus oocyte complexes exposed to PrOH protocols	1325:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	11	19	theme	minimum	1405:1411	arg1	oocytes					1397:1403	The oocytes minimum volume and permeability	1393:1435	The oocytes minimum volume and permeability	1393:1435	The oocytes minimum volume and permeability were affected by the exposure to PrOH and Ca2+ (p ≤ 0.007).					
29524383	11	19	theme	minimum	1405:1411	arg1	volume					1413:1418	minimum volume	1405:1418	minimum volume	1405:1418	The oocytes minimum volume and permeability were affected by the exposure to PrOH and Ca2+ (p ≤ 0.007).					
29524383	12	20	theme	concentration	1620:1632	arg1	DMSO					1592:1595	DMSO	1592:1595	DMSO	1592:1595	In conclusion, the most effective protocol for bovine oocytes cryopreservation combines EG and DMSO, independently of Ca2+ concentration in the media.					
29524383	12	20	theme	concentration	1620:1632	arg1	EG					1585:1586	EG	1585:1586	EG	1585:1586	In conclusion, the most effective protocol for bovine oocytes cryopreservation combines EG and DMSO, independently of Ca2+ concentration in the media.					
29524383	13	21	theme	oocyte	1734:1739	arg1	viability					1741:1749	oocyte viability	1734:1749	oocyte viability	1734:1749	A higher toxicity and an incomplete depletion of water during PrOH loading may hamper oocyte viability.					
29524383	0	22	theme	oocyte	7:12	arg1	permeability					23:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	22	theme	oocyte	7:12	arg1	Effects					54:60	Effects	54:60	Effects of different cryoprotectants and calcium in the vitrification media	54:128	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	23	theme	different	65:73	arg1	cryoprotectants					75:89	different cryoprotectants	65:89	different cryoprotectants	65:89	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	10	24	theme	vitrified	1232:1240	arg1	oocytes					1242:1248	vitrified oocytes	1232:1248	vitrified oocytes	1232:1248	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	1	25	theme	oocyte	188:193	arg1	cryosurvival					195:206	oocyte cryosurvival	188:206	oocyte cryosurvival	188:206	The cryopreservation process must be improved to enhance oocyte cryosurvival and functionality.					
29524383	0	26	theme	Bovine	0:5	arg1	permeability					23:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	26	theme	Bovine	0:5	arg1	Effects					54:60	Effects	54:60	Effects of different cryoprotectants and calcium in the vitrification media	54:128	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	2	27	contain	containing	280:289	arg1	1,2-propanediol					370:384	1,2-propanediol	370:384	1,2-propanediol	370:384	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	27	contain	containing	280:289	arg2	glycol					307:312	either ethylene glycol	291:312	either ethylene glycol (EG)	291:317	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	27	contain	containing	280:289	arg2	EG					315:316	EG	315:316	EG	315:316	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	27	contain	containing	280:289	arg1	cryoprotectants					256:270	different cryoprotectants	246:270	different cryoprotectants (CPAs)	246:277	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	27	contain	containing	280:289	arg1	sucrose					350:356	sucrose	350:356	sucrose	350:356	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	27	contain	containing	280:289	arg1	sulfoxide					329:337	dimethyl sulfoxide	320:337	dimethyl sulfoxide (DMSO)	320:344	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	27	contain	containing	280:289	arg1	CPAs					273:276	CPAs	273:276	CPAs	273:276	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	8	28	theme	higher	984:989	arg1	rates					1000:1004	higher cleavage rates	984:1004	higher cleavage rates	984:1004	After warming, higher cleavage rates were obtained in EGDMSO + Ca2+ compared to the PrOH without Ca2+ protocol (p = 0.02).					
29524383	10	29	from	acids	1295:1299	arg1	complexes					1340:1348	cumulus oocyte complexes	1325:1348	cumulus oocyte complexes exposed to PrOH protocols	1325:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	13	30	theme	PrOH	1710:1713	arg1	loading					1715:1721	PrOH loading	1710:1721	PrOH loading	1710:1721	A higher toxicity and an incomplete depletion of water during PrOH loading may hamper oocyte viability.					
29524383	2	31	with	protocols	231:239	arg1	CPAs					273:276	CPAs	273:276	CPAs	273:276	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	31	with	protocols	231:239	arg1	1,2-propanediol					370:384	1,2-propanediol	370:384	1,2-propanediol	370:384	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	31	with	protocols	231:239	arg1	cryoprotectants					256:270	different cryoprotectants	246:270	different cryoprotectants (CPAs)	246:277	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	31	with	protocols	231:239	arg1	sucrose					350:356	sucrose	350:356	sucrose	350:356	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	31	with	protocols	231:239	arg1	sulfoxide					329:337	dimethyl sulfoxide	320:337	dimethyl sulfoxide (DMSO)	320:344	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	10	32	theme	PrOH	1361:1364	arg1	protocols					1366:1374	PrOH protocols	1361:1374	PrOH protocols	1361:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	9	33	theme	lower	1139:1143	arg1	rates					1154:1158	lower cleavage rates	1139:1158	lower cleavage rates	1139:1158	Oocytes exposed to PrOH without Ca2+ presented lower cleavage rates compared to control (p = 0.04).					
29524383	4	34	theme	liquid	584:589	arg1	nitrogen					591:598	liquid nitrogen	584:598	liquid nitrogen	584:598	Oocytes were subjected to vitrification or only exposed to CPAs without immersion in liquid nitrogen.					
29524383	0	35	theme	membrane	14:21	arg1	permeability					23:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	35	theme	membrane	14:21	arg1	Effects					54:60	Effects	54:60	Effects of different cryoprotectants and calcium in the vitrification media	54:128	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	11	36	dep	oocytes	1397:1403	arg1	oocytes					1397:1403	The oocytes minimum volume and permeability	1393:1435	The oocytes minimum volume and permeability	1393:1435	The oocytes minimum volume and permeability were affected by the exposure to PrOH and Ca2+ (p ≤ 0.007).					
29524383	11	36	dep	oocytes	1397:1403	arg1	permeability					1424:1435	permeability	1424:1435	permeability	1424:1435	The oocytes minimum volume and permeability were affected by the exposure to PrOH and Ca2+ (p ≤ 0.007).					
29524383	11	36	dep	oocytes	1397:1403	arg1	volume					1413:1418	minimum volume	1405:1418	minimum volume	1405:1418	The oocytes minimum volume and permeability were affected by the exposure to PrOH and Ca2+ (p ≤ 0.007).					
29524383	10	37	from	hardening	1219:1227	arg1	oocytes					1242:1248	vitrified oocytes	1232:1248	vitrified oocytes	1232:1248	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	37	from	hardening	1219:1227	arg1	complexes					1340:1348	cumulus oocyte complexes	1325:1348	cumulus oocyte complexes exposed to PrOH protocols	1325:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	38	theme	lower	1261:1265	arg1	concentrations					1267:1280	lower concentrations	1261:1280	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1192:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	8	39	theme	Ca2+	1066:1069	arg1	p = 0.02					1081:1088	p = 0.02	1081:1088	p = 0.02	1081:1088	After warming, higher cleavage rates were obtained in EGDMSO + Ca2+ compared to the PrOH without Ca2+ protocol (p = 0.02).					
29524383	8	39	theme	Ca2+	1066:1069	arg1	protocol					1071:1078	Ca2+ protocol	1066:1078	Ca2+ protocol (p = 0.02)	1066:1089	After warming, higher cleavage rates were obtained in EGDMSO + Ca2+ compared to the PrOH without Ca2+ protocol (p = 0.02).					
29524383	0	40	dep	Effects	54:60	arg1	cryosurvival					40:51	cryosurvival	40:51	cryosurvival	40:51	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	40	dep	Effects	54:60	arg1	permeability					23:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability	0:34	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	0	40	dep	Effects	54:60	arg1	Effects					54:60	Effects	54:60	Effects of different cryoprotectants and calcium in the vitrification media	54:128	Bovine oocyte membrane permeability and cryosurvival: Effects of different cryoprotectants and calcium in the vitrification media.					
29524383	10	41	dep	acids	1295:1299	arg1	acids					1295:1299	the fatty acids c11:18:1 and 20:4n-6	1285:1320	the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1285:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	41	dep	acids	1295:1299	arg1	20:4n-6					1314:1320	20:4n-6	1314:1320	20:4n-6	1314:1320	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	41	dep	acids	1295:1299	arg1	c11:18:1					1301:1308	c11:18:1	1301:1308	c11:18:1	1301:1308	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	42	theme	enhanced	1195:1202	arg1	hardening					1219:1227	An enhanced premature zona hardening	1192:1227	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1192:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	12	43	theme	effective	1521:1529	arg1	protocol					1531:1538	the most effective protocol	1512:1538	the most effective protocol for bovine oocytes cryopreservation	1512:1574	In conclusion, the most effective protocol for bovine oocytes cryopreservation combines EG and DMSO, independently of Ca2+ concentration in the media.					
29524383	13	44	theme	incomplete	1673:1682	arg1	depletion					1684:1692	an incomplete depletion	1670:1692	an incomplete depletion of water during PrOH loading	1670:1721	A higher toxicity and an incomplete depletion of water during PrOH loading may hamper oocyte viability.					
29524383	2	45	theme	different	246:254	arg1	CPAs					273:276	CPAs	273:276	CPAs	273:276	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	45	theme	different	246:254	arg1	1,2-propanediol					370:384	1,2-propanediol	370:384	1,2-propanediol	370:384	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	45	theme	different	246:254	arg1	cryoprotectants					256:270	different cryoprotectants	246:270	different cryoprotectants (CPAs)	246:277	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	45	theme	different	246:254	arg1	sucrose					350:356	sucrose	350:356	sucrose	350:356	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	2	45	theme	different	246:254	arg1	sulfoxide					329:337	dimethyl sulfoxide	320:337	dimethyl sulfoxide (DMSO)	320:344	Two protocols with different cryoprotectants (CPAs), containing either ethylene glycol (EG), dimethyl sulfoxide (DMSO) and sucrose (EGDMSO) or 1,2-propanediol and sucrose (PrOH) were evaluated.					
29524383	6	46	theme	acid	729:732	arg1	composition					734:744	fatty acid composition	723:744	fatty acid composition	723:744	Finally, fatty acid composition and membrane permeability of oocytes exposed to CPAs were analyzed.					
29524383	5	47	theme	cortical	621:628	arg1	granules					630:637	cortical granules	621:637	cortical granules location	621:646	Oocyte's viability, cortical granules location and competence for development after fertilization were assessed.					
29524383	12	48	theme	bovine	1544:1549	arg1	cryopreservation					1559:1574	bovine oocytes cryopreservation	1544:1574	bovine oocytes cryopreservation	1544:1574	In conclusion, the most effective protocol for bovine oocytes cryopreservation combines EG and DMSO, independently of Ca2+ concentration in the media.					
29524383	6	49	theme	fatty	723:727	arg1	composition					734:744	fatty acid composition	723:744	fatty acid composition	723:744	Finally, fatty acid composition and membrane permeability of oocytes exposed to CPAs were analyzed.					
29524383	10	50	from	complexes	1340:1348	arg1	concentrations					1267:1280	lower concentrations	1261:1280	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1192:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	50	from	complexes	1340:1348	arg1	hardening					1219:1227	An enhanced premature zona hardening	1192:1227	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1192:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	5	51	theme	granules	630:637	arg1	location					639:646	cortical granules location	621:646	cortical granules location	621:646	Oocyte's viability, cortical granules location and competence for development after fertilization were assessed.					
29524383	10	52	theme	fatty	1289:1293	arg1	acids					1295:1299	the fatty acids c11:18:1 and 20:4n-6	1285:1320	the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols	1285:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	52	theme	fatty	1289:1293	arg1	20:4n-6					1314:1320	20:4n-6	1314:1320	20:4n-6	1314:1320	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	52	theme	fatty	1289:1293	arg1	c11:18:1					1301:1308	c11:18:1	1301:1308	c11:18:1	1301:1308	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	14	53	theme	oocyte	1807:1812	arg1	pathways					1814:1821	oocyte pathways	1807:1821	oocyte pathways to functionality	1807:1838	The type of CPAs and Ca2+ interfered differentially on oocyte pathways to functionality, and this should be considered when choosing a cryopreservation protocol.					
29524383	7	54	theme	development	882:892	arg1	higher					905:910	higher	905:910	higher	905:910	Independently of Ca2+ concentration in the vitrification media, the development rates were higher in oocytes vitrified with EGDMSO protocols (p = 0.0005).					
29524383	7	54	theme	development	882:892	arg1	rates					894:898	the development rates	878:898	the development rates	878:898	Independently of Ca2+ concentration in the vitrification media, the development rates were higher in oocytes vitrified with EGDMSO protocols (p = 0.0005).					
29524383	10	55	from	concentrations	1267:1280	arg1	oocytes					1242:1248	vitrified oocytes	1232:1248	vitrified oocytes	1232:1248	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	10	55	from	concentrations	1267:1280	arg1	complexes					1340:1348	cumulus oocyte complexes	1325:1348	cumulus oocyte complexes exposed to PrOH protocols	1325:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	14	56	theme	cryopreservation	1887:1902	arg1	protocol					1904:1911	a cryopreservation protocol	1885:1911	a cryopreservation protocol	1885:1911	The type of CPAs and Ca2+ interfered differentially on oocyte pathways to functionality, and this should be considered when choosing a cryopreservation protocol.					
29524383	12	57	theme	oocytes	1551:1557	arg1	cryopreservation					1559:1574	bovine oocytes cryopreservation	1544:1574	bovine oocytes cryopreservation	1544:1574	In conclusion, the most effective protocol for bovine oocytes cryopreservation combines EG and DMSO, independently of Ca2+ concentration in the media.					
29524383	10	58	theme	cumulus	1325:1331	arg1	complexes					1340:1348	cumulus oocyte complexes	1325:1348	cumulus oocyte complexes exposed to PrOH protocols	1325:1374	An enhanced premature zona hardening in vitrified oocytes as well as lower concentrations of the fatty acids c11:18:1 and 20:4n-6 in cumulus oocyte complexes exposed to PrOH protocols were identified.					
29524383	1	59	theme	cryopreservation	135:150	arg1	process					152:158	The cryopreservation process	131:158	The cryopreservation process	131:158	The cryopreservation process must be improved to enhance oocyte cryosurvival and functionality.					
29524383	13	60	theme	higher	1650:1655	arg1	toxicity					1657:1664	A higher toxicity	1648:1664	A higher toxicity	1648:1664	A higher toxicity and an incomplete depletion of water during PrOH loading may hamper oocyte viability.					
29524383	4	61	from	immersion	571:579	arg1	nitrogen					591:598	liquid nitrogen	584:598	liquid nitrogen	584:598	Oocytes were subjected to vitrification or only exposed to CPAs without immersion in liquid nitrogen.					
29524383	13	62	theme	water	1697:1701	arg1	depletion					1684:1692	an incomplete depletion	1670:1692	an incomplete depletion of water during PrOH loading	1670:1721	A higher toxicity and an incomplete depletion of water during PrOH loading may hamper oocyte viability.					
29524383	13	62	theme	water	1697:1701	arg1	toxicity					1657:1664	A higher toxicity	1648:1664	A higher toxicity	1648:1664	A higher toxicity and an incomplete depletion of water during PrOH loading may hamper oocyte viability.					
29524383	3	63	theme	-containing	463:473	arg1	media					480:484	calcium (Ca2+) free or -containing base media	440:484	calcium (Ca2+) free or -containing base media	440:484	In both protocols, calcium (Ca2+) free or -containing base media were tested.					
29524383	14	64	theme	Ca2+	1773:1776	arg1	type					1756:1759	The type	1752:1759	The type of CPAs and Ca2+	1752:1776	The type of CPAs and Ca2+ interfered differentially on oocyte pathways to functionality, and this should be considered when choosing a cryopreservation protocol.					
29524383	7	65	from	concentration	836:848	arg1	media					871:875	the vitrification media	853:875	the vitrification media	853:875	Independently of Ca2+ concentration in the vitrification media, the development rates were higher in oocytes vitrified with EGDMSO protocols (p = 0.0005).					
29524383	3	66	theme	base	475:478	arg1	media					480:484	calcium (Ca2+) free or -containing base media	440:484	calcium (Ca2+) free or -containing base media	440:484	In both protocols, calcium (Ca2+) free or -containing base media were tested.					
29524383	7	67	theme	vitrification	857:869	arg1	media					871:875	the vitrification media	853:875	the vitrification media	853:875	Independently of Ca2+ concentration in the vitrification media, the development rates were higher in oocytes vitrified with EGDMSO protocols (p = 0.0005).					
29705105	9	0	theme	drug	1563:1566	arg1	release					1568:1574	drug release	1563:1574	drug release	1563:1574	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	6	1	theme	resulting	870:878	arg1	bioinks					880:886	The resulting bioinks	866:886	The resulting bioinks	866:886	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	1	2	theme	common	151:156	arg1	extrusion					124:132	material extrusion	115:132	material extrusion	115:132	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	2	theme	common	151:156	arg1	jetting					103:109	Binder jetting	96:109	Binder jetting	96:109	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	2	theme	common	151:156	arg1	techniques					181:190	the two most common additive manufacturing techniques	138:190	the two most common additive manufacturing techniques used to create pharmaceutical tablets	138:228	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	4	3	theme	materials	616:624	arg1	number					606:611	a limited number	596:611	a limited number of materials that are both printable and biocompatible	596:666	Unfortunately, there are a limited number of materials that are both printable and biocompatible.					
29705105	10	4	theme	inkjet	1840:1845	arg1	printing					1847:1854	inkjet printing	1840:1854	inkjet printing with personalized drug dosages	1840:1885	These bioinks have the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages.					
29705105	0	5	theme	drugs	89:93	arg1	pharming					65:72	the inkjet 3D pharming	51:72	the inkjet 3D pharming of hydrophobic drugs	51:93	Photocurable poly(ethylene glycol) as a bioink for the inkjet 3D pharming of hydrophobic drugs.					
29705105	1	6	theme	manufacturing	167:179	arg1	extrusion					124:132	material extrusion	115:132	material extrusion	115:132	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	6	theme	manufacturing	167:179	arg1	jetting					103:109	Binder jetting	96:109	Binder jetting	96:109	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	6	theme	manufacturing	167:179	arg1	techniques					181:190	the two most common additive manufacturing techniques	138:190	the two most common additive manufacturing techniques used to create pharmaceutical tablets	138:228	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	5	7	contain	have	811:814	arg2	times					831:835	rapid gelation times	816:835	rapid gelation times	816:835	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	5	7	contain	have	811:814	arg1	bioinks					731:737	photocurable bioinks	718:737	photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure	718:863	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	6	8	theme	ethylene	1028:1035	arg1	glycol					1037:1042	ethylene glycol	1028:1042	ethylene glycol	1028:1042	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	6	8	theme	ethylene	1028:1035	arg1	-amine					1044:1049	methoxide-poly(ethylene glycol)-amine	1013:1049	methoxide-poly(ethylene glycol)-amine as the coinitiator	1013:1068	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	5	9	theme	study	696:700	arg1	aim					684:686	the aim	680:686	the aim of this study	680:700	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	5	10	theme	visible	842:848	arg1	exposure					856:863	visible light exposure	842:863	visible light exposure	842:863	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	9	11	theme	poly	1616:1619	arg1	diacrylate					1638:1647	poly(ethylene glycol) diacrylate	1616:1647	poly(ethylene glycol) diacrylate in the formulation	1616:1666	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	10	12	theme	drug	1874:1877	arg1	dosages					1879:1885	personalized drug dosages	1861:1885	personalized drug dosages	1861:1885	These bioinks have the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages.					
29705105	9	13	theme	ethylene	1621:1628	arg1	poly					1616:1619	poly	1616:1619	poly(ethylene glycol) diacrylate in the formulation	1616:1666	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	9	13	theme	ethylene	1621:1628	arg1	glycol					1630:1635	ethylene glycol	1621:1635	ethylene glycol	1621:1635	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	7	14	theme	release	1164:1170	arg1	profiles					1172:1179	the drug release profiles	1155:1179	the drug release profiles	1155:1179	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	1	15	theme	material	115:122	arg1	techniques					181:190	the two most common additive manufacturing techniques	138:190	the two most common additive manufacturing techniques used to create pharmaceutical tablets	138:228	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	15	theme	material	115:122	arg1	jetting					103:109	Binder jetting	96:109	Binder jetting	96:109	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	15	theme	material	115:122	arg1	extrusion					124:132	material extrusion	115:132	material extrusion	115:132	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	8	16	theme	bioink	1262:1267	arg1	formulations					1269:1280	Various bioink formulations	1254:1280	Various bioink formulations	1254:1280	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	0	17	theme	3D	62:63	arg1	pharming					65:72	the inkjet 3D pharming	51:72	the inkjet 3D pharming of hydrophobic drugs	51:93	Photocurable poly(ethylene glycol) as a bioink for the inkjet 3D pharming of hydrophobic drugs.					
29705105	6	18	theme	crosslinkable	955:967	arg1	monomer					969:975	the crosslinkable monomer	951:975	the crosslinkable monomer	951:975	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	3	19	theme	material	457:464	arg1	jetting					466:472	material jetting	457:472	material jetting	457:472	Conversely, material jetting allows for compositional control at a voxel level and can dispense material at room temperature.					
29705105	5	20	theme	photocurable	718:729	arg1	bioinks					731:737	photocurable bioinks	718:737	photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure	718:863	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	9	21	theme	exposure	1682:1689	arg1	time					1691:1694	the light exposure time	1672:1694	the light exposure time used to cure the bioinks	1672:1719	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	8	22	theme	top	1443:1445	arg1	half					1447:1450	the top half	1439:1450	the top half of the preform tablet	1439:1472	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	2	23	theme	3D	360:361	arg1	printing					363:370	binder jet 3D printing	349:370	binder jet 3D printing	349:370	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	0	24	theme	Photocurable	0:11	arg1	poly					13:16	Photocurable poly	0:16	Photocurable poly(ethylene glycol)	0:33	Photocurable poly(ethylene glycol) as a bioink for the inkjet 3D pharming of hydrophobic drugs.					
29705105	0	24	theme	Photocurable	0:11	arg1	glycol					27:32	ethylene glycol	18:32	ethylene glycol	18:32	Photocurable poly(ethylene glycol) as a bioink for the inkjet 3D pharming of hydrophobic drugs.					
29705105	7	25	theme	high	1233:1236	arg1	hydrophobicity					1238:1251	its high hydrophobicity	1229:1251	its high hydrophobicity	1229:1251	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	2	26	theme	high	416:419	arg1	temperatures					431:442	high operating temperatures	416:442	high operating temperatures	416:442	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	8	27	theme	bottom	1306:1311	arg1	half					1313:1316	the bottom half	1302:1316	the bottom half of blank preform tablets	1302:1341	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	0	28	dep	poly	13:16	arg1	bioink					40:45	a bioink	38:45	a bioink for the inkjet 3D pharming of hydrophobic drugs	38:93	Photocurable poly(ethylene glycol) as a bioink for the inkjet 3D pharming of hydrophobic drugs.					
29705105	5	29	theme	rapid	816:820	arg1	times					831:835	rapid gelation times	816:835	rapid gelation times	816:835	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	6	30	theme	methoxide-poly	1013:1026	arg1	glycol					1037:1042	ethylene glycol	1028:1042	ethylene glycol	1028:1042	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	6	30	theme	methoxide-poly	1013:1026	arg1	-amine					1044:1049	methoxide-poly(ethylene glycol)-amine	1013:1049	methoxide-poly(ethylene glycol)-amine as the coinitiator	1013:1068	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	8	31	theme	preform	1459:1465	arg1	tablet					1467:1472	the preform tablet	1455:1472	the preform tablet	1455:1472	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	7	32	used	used	1199:1202	arg2	Naproxen					1186:1193	Naproxen	1186:1193	Naproxen	1186:1193	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	7	32	used	used	1199:1202	arg2	drug					1217:1220	the model drug	1207:1220	the model drug due to its high hydrophobicity	1207:1251	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	2	33	theme	extrusion	385:393	arg1	printing					398:405	material extrusion 3D printing	376:405	material extrusion 3D printing	376:405	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	5	34	theme	hydrophobic	761:771	arg1	ingredients					795:805	hydrophobic active pharmaceutical ingredients	761:805	hydrophobic active pharmaceutical ingredients	761:805	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	9	35	from	formulation	1656:1666	arg1	percent					1605:1611	the percent	1601:1611	the percent of poly(ethylene glycol) diacrylate in the formulation	1601:1666	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	9	35	from	formulation	1656:1666	arg1	time					1691:1694	the light exposure time	1672:1694	the light exposure time used to cure the bioinks	1672:1719	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	8	36	theme	preform	1327:1333	arg1	tablets					1335:1341	blank preform tablets	1321:1341	blank preform tablets	1321:1341	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	2	37	theme	dosage	322:327	arg1	forms					329:333	the dosage forms	318:333	the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures	318:442	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	5	38	theme	pharmaceutical	780:793	arg1	ingredients					795:805	hydrophobic active pharmaceutical ingredients	761:805	hydrophobic active pharmaceutical ingredients	761:805	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	10	39	contain	have	1736:1739	arg1	bioinks					1728:1734	These bioinks	1722:1734	These bioinks	1722:1734	These bioinks have the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages.					
29705105	10	39	contain	have	1736:1739	arg2	potential					1745:1753	the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages	1741:1885	the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages	1741:1885	These bioinks have the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages.					
29705105	8	40	theme	dosage	1505:1510	arg1	form					1512:1515	the pharmaceutical dosage form	1486:1515	the pharmaceutical dosage form	1486:1515	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	2	41	theme	binder	349:354	arg1	printing					363:370	binder jet 3D printing	349:370	binder jet 3D printing	349:370	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	1	42	theme	pharmaceutical	207:220	arg1	tablets					222:228	pharmaceutical tablets	207:228	pharmaceutical tablets	207:228	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	5	43	theme	active	773:778	arg1	ingredients					795:805	hydrophobic active pharmaceutical ingredients	761:805	hydrophobic active pharmaceutical ingredients	761:805	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	6	44	theme	ethylene	911:918	arg1	poly					906:909	poly	906:909	poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator	906:1068	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	6	44	theme	ethylene	911:918	arg1	glycol					920:925	ethylene glycol	911:925	ethylene glycol	911:925	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	4	45	theme	limited	598:604	arg1	number					606:611	a limited number	596:611	a limited number of materials that are both printable and biocompatible	596:666	Unfortunately, there are a limited number of materials that are both printable and biocompatible.					
29705105	10	46	theme	materials	1780:1788	arg1	library					1769:1775	the library	1765:1775	the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages	1765:1885	These bioinks have the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages.					
29705105	9	47	from	time	1691:1694	arg1	formulation					1656:1666	the formulation	1652:1666	the formulation	1652:1666	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	8	48	theme	direct	1354:1359	arg1	compression					1361:1371	direct compression	1354:1371	direct compression	1354:1371	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	6	49	theme	poly	906:909	arg1	diacrylate					928:937	poly(ethylene glycol) diacrylate	906:937	poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator	906:1068	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	6	49	theme	poly	906:909	arg1	250 Da					940:945	250 Da	940:945	250 Da	940:945	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	10	50	theme	pharmaceutical	1813:1826	arg1	tablets					1828:1834	pharmaceutical tablets	1813:1834	pharmaceutical tablets	1813:1834	These bioinks have the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages.					
29705105	5	51	theme	light	850:854	arg1	exposure					856:863	visible light exposure	842:863	visible light exposure	842:863	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	9	52	from	percent	1605:1611	arg1	formulation					1656:1666	the formulation	1652:1666	the formulation	1652:1666	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	3	53	theme	room	553:556	arg1	temperature					558:568	room temperature	553:568	room temperature	553:568	Conversely, material jetting allows for compositional control at a voxel level and can dispense material at room temperature.					
29705105	2	54	theme	powder	279:284	arg1	present					299:305	present	299:305	present	299:305	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	2	54	theme	powder	279:284	arg1	component					286:294	the powder component	275:294	the powder component	275:294	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	5	55	theme	gelation	822:829	arg1	times					831:835	rapid gelation times	816:835	rapid gelation times	816:835	Therefore, the aim of this study was to engineer photocurable bioinks that are suitable for hydrophobic active pharmaceutical ingredients and have rapid gelation times upon visible light exposure.					
29705105	8	56	theme	piezoelectric	1383:1395	arg1	nozzle					1397:1402	a piezoelectric nozzle	1381:1402	a piezoelectric nozzle	1381:1402	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	10	57	with	printing	1847:1854	arg1	dosages					1879:1885	personalized drug dosages	1861:1885	personalized drug dosages	1861:1885	These bioinks have the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages.					
29705105	10	58	theme	personalized	1861:1872	arg1	dosages					1879:1885	personalized drug dosages	1861:1885	personalized drug dosages	1861:1885	These bioinks have the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages.					
29705105	1	59	theme	Binder	96:101	arg1	extrusion					124:132	material extrusion	115:132	material extrusion	115:132	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	59	theme	Binder	96:101	arg1	jetting					103:109	Binder jetting	96:109	Binder jetting	96:109	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	59	theme	Binder	96:101	arg1	techniques					181:190	the two most common additive manufacturing techniques	138:190	the two most common additive manufacturing techniques used to create pharmaceutical tablets	138:228	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	7	60	theme	drug	1159:1162	arg1	profiles					1172:1179	the drug release profiles	1155:1179	the drug release profiles	1155:1179	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	1	61	theme	additive	158:165	arg1	extrusion					124:132	material extrusion	115:132	material extrusion	115:132	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	61	theme	additive	158:165	arg1	jetting					103:109	Binder jetting	96:109	Binder jetting	96:109	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	1	61	theme	additive	158:165	arg1	techniques					181:190	the two most common additive manufacturing techniques	138:190	the two most common additive manufacturing techniques used to create pharmaceutical tablets	138:228	Binder jetting and material extrusion are the two most common additive manufacturing techniques used to create pharmaceutical tablets.					
29705105	9	62	theme	diacrylate	1638:1647	arg1	percent					1605:1611	the percent	1601:1611	the percent of poly(ethylene glycol) diacrylate in the formulation	1601:1666	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	9	62	theme	diacrylate	1638:1647	arg1	time					1691:1694	the light exposure time	1672:1694	the light exposure time used to cure the bioinks	1672:1719	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	0	63	theme	inkjet	55:60	arg1	pharming					65:72	the inkjet 3D pharming	51:72	the inkjet 3D pharming of hydrophobic drugs	51:93	Photocurable poly(ethylene glycol) as a bioink for the inkjet 3D pharming of hydrophobic drugs.					
29705105	6	64	theme	Eosin	978:982	arg1	Y					984:984	Eosin Y	978:984	Eosin Y as the photoinitiator	978:1006	The resulting bioinks were comprised of poly(ethylene glycol) diacrylate (250 Da) as the crosslinkable monomer, Eosin Y as the photoinitiator, and methoxide-poly(ethylene glycol)-amine as the coinitiator.					
29705105	8	65	theme	Various	1254:1260	arg1	formulations					1269:1280	Various bioink formulations	1254:1280	Various bioink formulations	1254:1280	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	0	66	theme	hydrophobic	77:87	arg1	drugs					89:93	hydrophobic drugs	77:93	hydrophobic drugs	77:93	Photocurable poly(ethylene glycol) as a bioink for the inkjet 3D pharming of hydrophobic drugs.					
29705105	9	67	from	studies	1543:1549	arg1	Results					1518:1524	Results	1518:1524	Results from the release studies	1518:1549	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	9	68	theme	light	1676:1680	arg1	time					1691:1694	the light exposure time	1672:1694	the light exposure time used to cure the bioinks	1672:1719	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	2	69	theme	operating	421:429	arg1	temperatures					431:442	high operating temperatures	416:442	high operating temperatures	416:442	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	2	70	theme	jet	356:358	arg1	printing					363:370	binder jet 3D printing	349:370	binder jet 3D printing	349:370	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	0	71	theme	ethylene	18:25	arg1	poly					13:16	Photocurable poly	0:16	Photocurable poly(ethylene glycol)	0:33	Photocurable poly(ethylene glycol) as a bioink for the inkjet 3D pharming of hydrophobic drugs.					
29705105	0	71	theme	ethylene	18:25	arg1	glycol					27:32	ethylene glycol	18:32	ethylene glycol	18:32	Photocurable poly(ethylene glycol) as a bioink for the inkjet 3D pharming of hydrophobic drugs.					
29705105	8	72	dep	nozzle	1397:1402	arg1	photopolymerized					1405:1420	photopolymerized	1405:1420	photopolymerized	1405:1420	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	8	72	dep	nozzle	1397:1402	arg1	capped					1427:1432	capped	1427:1432	capped with the top half of the preform tablet to complete the pharmaceutical dosage form	1427:1515	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	7	73	theme	due	1222:1224	arg1	Naproxen					1186:1193	Naproxen	1186:1193	Naproxen	1186:1193	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	7	73	theme	due	1222:1224	arg1	drug					1217:1220	the model drug	1207:1220	the model drug due to its high hydrophobicity	1207:1251	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	8	74	theme	tablet	1467:1472	arg1	half					1447:1450	the top half	1439:1450	the top half of the preform tablet	1439:1472	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	2	75	theme	3D	395:396	arg1	printing					398:405	material extrusion 3D printing	376:405	material extrusion 3D printing	376:405	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	7	76	theme	model	1211:1215	arg1	Naproxen					1186:1193	Naproxen	1186:1193	Naproxen	1186:1193	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	7	76	theme	model	1211:1215	arg1	drug					1217:1220	the model drug	1207:1220	the model drug due to its high hydrophobicity	1207:1251	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	2	77	theme	material	376:383	arg1	printing					398:405	material extrusion 3D printing	376:405	material extrusion 3D printing	376:405	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	7	78	theme	ethylene	1090:1097	arg1	poly					1085:1088	poly	1085:1088	poly(ethylene glycol) (200 Da)	1085:1114	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	7	78	theme	ethylene	1090:1097	arg1	glycol					1099:1104	ethylene glycol	1090:1104	ethylene glycol	1090:1104	Additionally, poly(ethylene glycol) (200 Da) was added as a plasticizer to modulate the drug release profiles, and Naproxen was used as the model drug due to its high hydrophobicity.					
29705105	8	79	theme	pharmaceutical	1490:1503	arg1	form					1512:1515	the pharmaceutical dosage form	1486:1515	the pharmaceutical dosage form	1486:1515	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	8	80	theme	blank	1321:1325	arg1	tablets					1335:1341	blank preform tablets	1321:1341	blank preform tablets	1321:1341	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	3	81	theme	compositional	485:497	arg1	control					499:505	compositional control	485:505	compositional control	485:505	Conversely, material jetting allows for compositional control at a voxel level and can dispense material at room temperature.					
29705105	8	82	theme	tablets	1335:1341	arg1	half					1313:1316	the bottom half	1302:1316	the bottom half of blank preform tablets	1302:1341	Various bioink formulations were dispensed into the bottom half of blank preform tablets - made via direct compression - using a piezoelectric nozzle, photopolymerized, and capped with the top half of the preform tablet to complete the pharmaceutical dosage form.					
29705105	9	83	from	diacrylate	1638:1647	arg1	formulation					1656:1666	the formulation	1652:1666	the formulation	1652:1666	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	9	84	theme	release	1535:1541	arg1	studies					1543:1549	the release studies	1531:1549	the release studies	1531:1549	Results from the release studies showed that drug release can be modulated by both the percent of poly(ethylene glycol) diacrylate in the formulation and the light exposure time used to cure the bioinks.					
29705105	10	85	theme	available	1790:1798	arg1	materials					1780:1788	materials	1780:1788	materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages	1780:1885	These bioinks have the potential to expand the library of materials available for creating pharmaceutical tablets via inkjet printing with personalized drug dosages.					
29705105	2	86	dep	printing	363:370	arg1	requires					407:414	requires	407:414	requires high operating temperatures	407:442	However, their versatility is limited since the powder component is present throughout the dosage forms fabricated by binder jet 3D printing and material extrusion 3D printing requires high operating temperatures.					
29705105	3	87	theme	voxel	512:516	arg1	level					518:522	a voxel level	510:522	a voxel level	510:522	Conversely, material jetting allows for compositional control at a voxel level and can dispense material at room temperature.					
29311037	4	0	theme	sacrosidase	649:659	arg1	dose					661:664	sacrosidase dose	649:664	sacrosidase dose	649:664	OBJECTIVE To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms.					
29311037	13	1	theme	44	1645:1646	arg1	%					1647:1647	%	1647:1647	%	1647:1647	Macronutrient composition averaged 44% carbohydrate, 39% fat, and 17% protein.					
29311037	18	2	theme	Macronutrient	2038:2050	arg1	Ranges					2065:2070	the Acceptable Macronutrient Distribution Ranges	2023:2070	the Acceptable Macronutrient Distribution Ranges	2023:2070	CONCLUSIONS Intakes were lower in carbohydrates and higher in fat compared with the Acceptable Macronutrient Distribution Ranges.					
29311037	7	3	theme	nutrient	982:989	arg1	intakes					991:997	Dietary energy and nutrient intakes	963:997	intakes	991:997	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	7	4	theme	diet	1023:1026	arg1	interviews					1035:1044	24-hour diet recall interviews	1015:1044	24-hour diet recall interviews	1015:1044	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	14	5	dep	%	1728:1728	arg1	starch					1730:1735	starch	1730:1735	35% starch	1726:1735	Average carbohydrate composition was 35% starch, 8% fiber, and 59% sugars.					
29311037	14	5	dep	%	1728:1728	arg1	sugars					1756:1761	sugars	1756:1761	sugars	1756:1761	Average carbohydrate composition was 35% starch, 8% fiber, and 59% sugars.					
29311037	5	6	theme	30-day	719:724	arg1	study					740:744	A prospective 30-day observational study	705:744	DESIGN A prospective 30-day observational study	698:744	DESIGN A prospective 30-day observational study.					
29311037	7	7	theme	MAIN	941:944	arg1	MEASURES					954:961	MAIN OUTCOME MEASURES	941:961	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.	941:1125	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	14	8	theme	Average	1689:1695	arg1	composition					1710:1720	Average carbohydrate composition	1689:1720	Average carbohydrate composition	1689:1720	Average carbohydrate composition was 35% starch, 8% fiber, and 59% sugars.					
29311037	7	9	theme	sacrosidase	1109:1119	arg1	dose					1121:1124	sacrosidase dose	1109:1124	sacrosidase dose	1109:1124	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	1	10	theme	Sucrose-isomaltase	170:187	arg1	SID					201:203	SID	201:203	SID	201:203	BACKGROUND Sucrose-isomaltase deficiency (SID) remains underdiagnosed.					
29311037	1	10	theme	Sucrose-isomaltase	170:187	arg1	deficiency					189:198	BACKGROUND Sucrose-isomaltase deficiency	159:198	BACKGROUND Sucrose-isomaltase deficiency (SID)	159:204	BACKGROUND Sucrose-isomaltase deficiency (SID) remains underdiagnosed.					
29311037	3	11	theme	gastrointestinal	391:406	arg1	symptoms					408:415	gastrointestinal symptoms	391:415	gastrointestinal symptoms	391:415	Frequency and severity of gastrointestinal symptoms may be associated with the type of carbohydrates consumed.					
29311037	4	12	theme	dietary	506:512	arg1	intakes					514:520	the dietary intakes	502:520	the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID	502:588	OBJECTIVE To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms.					
29311037	11	13	theme	sacrosidase	1453:1463	arg1	intake					1465:1470	higher sacrosidase intake	1446:1470	higher sacrosidase intake	1446:1470	Having less frequent GI symptoms was associated with higher sacrosidase intake.					
29311037	14	14	theme	8	1738:1738	arg1	%					1739:1739	%	1739:1739	%	1739:1739	Average carbohydrate composition was 35% starch, 8% fiber, and 59% sugars.					
29311037	20	15	theme	Higher	2140:2145	arg1	intakes					2167:2173	Higher maltose and lactose intakes	2140:2173	Higher maltose and lactose intakes	2140:2173	Higher maltose and lactose intakes were associated with GI symptom frequency and severity.					
29311037	0	16	from	Relationships	0:12	arg1	Patients					80:87	Patients	80:87	Patients Receiving Enzyme Treatment for Genetic Sucrase-Isomaltase Deficiency	80:156	Relationships among Dietary Intakes and Persistent Gastrointestinal Symptoms in Patients Receiving Enzyme Treatment for Genetic Sucrase-Isomaltase Deficiency.					
29311037	8	17	theme	nutrient	1180:1187	arg1	intakes					1189:1195	nutrient intakes	1180:1195	nutrient intakes	1180:1195	STATISTICAL ANALYSES PERFORMED Relationships between nutrient intakes, sacrosidase dose, and GI symptoms were evaluated using Spearman ρ correlation coefficients.					
29311037	20	18	theme	GI	2196:2197	arg1	frequency					2207:2215	GI symptom frequency	2196:2215	GI symptom frequency	2196:2215	Higher maltose and lactose intakes were associated with GI symptom frequency and severity.					
29311037	13	19	theme	17	1676:1677	arg1	%					1678:1678	%	1678:1678	%	1678:1678	Macronutrient composition averaged 44% carbohydrate, 39% fat, and 17% protein.					
29311037	0	20	theme	Genetic	120:126	arg1	Deficiency					147:156	Genetic Sucrase-Isomaltase Deficiency	120:156	Genetic Sucrase-Isomaltase Deficiency	120:156	Relationships among Dietary Intakes and Persistent Gastrointestinal Symptoms in Patients Receiving Enzyme Treatment for Genetic Sucrase-Isomaltase Deficiency.					
29311037	19	21	theme	fructose	2085:2092	arg1	intakes					2094:2100	Sucrose and fructose intakes	2073:2100	Sucrose and fructose intakes	2073:2100	Sucrose and fructose intakes were not associated with GI symptoms.					
29311037	19	22	theme	Sucrose	2073:2079	arg1	intakes					2094:2100	Sucrose and fructose intakes	2073:2100	Sucrose and fructose intakes	2073:2100	Sucrose and fructose intakes were not associated with GI symptoms.					
29311037	20	23	theme	maltose	2147:2153	arg1	intakes					2167:2173	Higher maltose and lactose intakes	2140:2173	Higher maltose and lactose intakes	2140:2173	Higher maltose and lactose intakes were associated with GI symptom frequency and severity.					
29311037	17	24	theme	Maltose	1876:1882	arg1	intake					1884:1889	Maltose intake	1876:1889	Maltose intake	1876:1889	Maltose intake was associated with nausea, distension, and reflux.					
29311037	13	25	theme	%	1665:1665	arg1	fat					1667:1669	39% fat	1663:1669	39% fat	1663:1669	Macronutrient composition averaged 44% carbohydrate, 39% fat, and 17% protein.					
29311037	0	26	theme	Dietary	20:26	arg1	Intakes					28:34	Dietary Intakes	20:34	Dietary Intakes	20:34	Relationships among Dietary Intakes and Persistent Gastrointestinal Symptoms in Patients Receiving Enzyme Treatment for Genetic Sucrase-Isomaltase Deficiency.					
29311037	20	27	theme	lactose	2159:2165	arg1	intakes					2167:2173	Higher maltose and lactose intakes	2140:2173	Higher maltose and lactose intakes	2140:2173	Higher maltose and lactose intakes were associated with GI symptom frequency and severity.					
29311037	6	28	theme	Forty-nine	768:777	arg1	patients					779:786	PARTICIPANTS/SETTING Forty-nine patients	747:786	PARTICIPANTS/SETTING Forty-nine patients treated with sacrosidase for ≥3 months	747:825	PARTICIPANTS/SETTING Forty-nine patients treated with sacrosidase for ≥3 months were recruited from the enzyme manufacturer's nationwide clinical database between November 2014 and August 2015.					
29311037	5	29	dep	DESIGN	698:703	arg1	study					740:744	A prospective 30-day observational study	705:744	DESIGN A prospective 30-day observational study	698:744	DESIGN A prospective 30-day observational study.					
29311037	13	30	theme	%	1647:1647	arg1	carbohydrate					1649:1660	44% carbohydrate	1645:1660	44% carbohydrate	1645:1660	Macronutrient composition averaged 44% carbohydrate, 39% fat, and 17% protein.					
29311037	0	31	theme	Gastrointestinal	51:66	arg1	Symptoms					68:75	Persistent Gastrointestinal Symptoms	40:75	Persistent Gastrointestinal Symptoms	40:75	Relationships among Dietary Intakes and Persistent Gastrointestinal Symptoms in Patients Receiving Enzyme Treatment for Genetic Sucrase-Isomaltase Deficiency.					
29311037	11	32	theme	GI	1414:1415	arg1	symptoms					1417:1424	less frequent GI symptoms	1400:1424	less frequent GI symptoms	1400:1424	Having less frequent GI symptoms was associated with higher sacrosidase intake.					
29311037	2	33	theme	enzyme	248:253	arg1	activity					255:262	Absent or reduced enzyme activity	230:262	Absent or reduced enzyme activity	230:262	Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides.					
29311037	9	34	theme	RESULTS	1290:1296	arg1	dose					1310:1313	RESULTS Sacrosidase dose	1290:1313	RESULTS Sacrosidase dose	1290:1313	RESULTS Sacrosidase dose averaged 5.2±3.1 mL/day.					
29311037	8	35	theme	Spearman	1253:1260	arg1	coefficients					1276:1287	Spearman ρ correlation coefficients	1253:1287	Spearman ρ correlation coefficients	1253:1287	STATISTICAL ANALYSES PERFORMED Relationships between nutrient intakes, sacrosidase dose, and GI symptoms were evaluated using Spearman ρ correlation coefficients.					
29311037	19	36	theme	GI	2127:2128	arg1	symptoms					2130:2137	GI symptoms	2127:2137	GI symptoms	2127:2137	Sucrose and fructose intakes were not associated with GI symptoms.					
29311037	15	37	theme	fructose	1776:1783	arg1	intakes					1785:1791	Sucrose and fructose intakes	1764:1791	Sucrose and fructose intakes	1764:1791	Sucrose and fructose intakes were not associated with GI symptoms.					
29311037	13	38	theme	Macronutrient	1610:1622	arg1	composition					1624:1634	Macronutrient composition	1610:1634	Macronutrient composition	1610:1634	Macronutrient composition averaged 44% carbohydrate, 39% fat, and 17% protein.					
29311037	8	39	theme	correlation	1264:1274	arg1	coefficients					1276:1287	Spearman ρ correlation coefficients	1253:1287	Spearman ρ correlation coefficients	1253:1287	STATISTICAL ANALYSES PERFORMED Relationships between nutrient intakes, sacrosidase dose, and GI symptoms were evaluated using Spearman ρ correlation coefficients.					
29311037	5	40	theme	observational	726:738	arg1	study					740:744	A prospective 30-day observational study	705:744	DESIGN A prospective 30-day observational study	698:744	DESIGN A prospective 30-day observational study.					
29311037	15	41	theme	Sucrose	1764:1770	arg1	intakes					1785:1791	Sucrose and fructose intakes	1764:1791	Sucrose and fructose intakes	1764:1791	Sucrose and fructose intakes were not associated with GI symptoms.					
29311037	18	42	theme	Distribution	2052:2063	arg1	Ranges					2065:2070	the Acceptable Macronutrient Distribution Ranges	2023:2070	the Acceptable Macronutrient Distribution Ranges	2023:2070	CONCLUSIONS Intakes were lower in carbohydrates and higher in fat compared with the Acceptable Macronutrient Distribution Ranges.					
29311037	2	43	theme	malabsorbed	338:348	arg1	disaccharides					350:362	undigested and malabsorbed disaccharides	323:362	undigested and malabsorbed disaccharides	323:362	Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides.					
29311037	18	44	theme	Acceptable	2027:2036	arg1	Ranges					2065:2070	the Acceptable Macronutrient Distribution Ranges	2023:2070	the Acceptable Macronutrient Distribution Ranges	2023:2070	CONCLUSIONS Intakes were lower in carbohydrates and higher in fat compared with the Acceptable Macronutrient Distribution Ranges.					
29311037	10	45	theme	bowel	1370:1374	arg1	movements					1376:1384	1.3±0.9 bowel movements	1362:1384	1.3±0.9 bowel movements	1362:1384	Participants reported 1.3±0.9 bowel movements daily.					
29311037	2	46	theme	undigested	323:332	arg1	disaccharides					350:362	undigested and malabsorbed disaccharides	323:362	undigested and malabsorbed disaccharides	323:362	Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides.					
29311037	8	47	theme	STATISTICAL	1127:1137	arg1	ANALYSES					1139:1146	STATISTICAL ANALYSES	1127:1146	STATISTICAL ANALYSES PERFORMED Relationships between nutrient intakes, sacrosidase dose, and GI symptoms	1127:1230	STATISTICAL ANALYSES PERFORMED Relationships between nutrient intakes, sacrosidase dose, and GI symptoms were evaluated using Spearman ρ correlation coefficients.					
29311037	5	48	theme	prospective	707:717	arg1	study					740:744	A prospective 30-day observational study	705:744	DESIGN A prospective 30-day observational study	698:744	DESIGN A prospective 30-day observational study.					
29311037	15	49	theme	GI	1818:1819	arg1	symptoms					1821:1828	GI symptoms	1818:1828	GI symptoms	1818:1828	Sucrose and fructose intakes were not associated with GI symptoms.					
29311037	7	50	theme	Dietary	963:969	arg1	energy					971:976	Dietary energy and nutrient intakes	963:997	energy	971:976	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	4	51	theme	patients	525:532	arg1	intakes					514:520	the dietary intakes	502:520	the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID	502:588	OBJECTIVE To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms.					
29311037	2	52	theme	abdominal	283:291	arg1	bloating					293:300	abdominal bloating	283:300	abdominal bloating	283:300	Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides.					
29311037	7	53	theme	OUTCOME	946:952	arg1	MEASURES					954:961	MAIN OUTCOME MEASURES	941:961	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.	941:1125	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	21	54	theme	nutrition	2272:2280	arg1	counseling					2282:2291	nutrition counseling	2272:2291	nutrition counseling for patients treated for SID	2272:2320	These findings provide evidence to guide nutrition counseling for patients treated for SID.					
29311037	6	55	theme	PARTICIPANTS/SETTING	747:766	arg1	patients					779:786	PARTICIPANTS/SETTING Forty-nine patients	747:786	PARTICIPANTS/SETTING Forty-nine patients treated with sacrosidase for ≥3 months	747:825	PARTICIPANTS/SETTING Forty-nine patients treated with sacrosidase for ≥3 months were recruited from the enzyme manufacturer's nationwide clinical database between November 2014 and August 2015.					
29311037	6	56	theme	clinical	884:891	arg1	database					893:900	the enzyme manufacturer's nationwide clinical database	847:900	the enzyme manufacturer's nationwide clinical database between November 2014 and August 2015	847:938	PARTICIPANTS/SETTING Forty-nine patients treated with sacrosidase for ≥3 months were recruited from the enzyme manufacturer's nationwide clinical database between November 2014 and August 2015.					
29311037	16	57	theme	Lactose	1831:1837	arg1	intake					1839:1844	Lactose intake	1831:1844	Lactose intake	1831:1844	Lactose intake was associated with diarrhea.					
29311037	1	58	theme	BACKGROUND	159:168	arg1	SID					201:203	SID	201:203	SID	201:203	BACKGROUND Sucrose-isomaltase deficiency (SID) remains underdiagnosed.					
29311037	1	58	theme	BACKGROUND	159:168	arg1	deficiency					189:198	BACKGROUND Sucrose-isomaltase deficiency	159:198	BACKGROUND Sucrose-isomaltase deficiency (SID)	159:204	BACKGROUND Sucrose-isomaltase deficiency (SID) remains underdiagnosed.					
29311037	12	59	from	kcal/day	1547:1554	arg1	adolescents					1559:1569	adolescents	1559:1569	adolescents	1559:1569	Energy intakes averaged 1,562.5±411.5 kcal/day in children, 1,964.7±823.6 kcal/day in adolescents, and 1,952.6±546.5 kcal/day in adults.					
29311037	6	60	theme	enzyme	851:856	arg1	manufacturer					858:869	enzyme manufacturer's	851:871	the enzyme manufacturer's nationwide clinical database between November 2014 and August 2015	847:938	PARTICIPANTS/SETTING Forty-nine patients treated with sacrosidase for ≥3 months were recruited from the enzyme manufacturer's nationwide clinical database between November 2014 and August 2015.					
29311037	3	61	theme	symptoms	408:415	arg1	severity					379:386	severity	379:386	severity	379:386	Frequency and severity of gastrointestinal symptoms may be associated with the type of carbohydrates consumed.					
29311037	3	61	theme	symptoms	408:415	arg1	Frequency					365:373	Frequency	365:373	Frequency	365:373	Frequency and severity of gastrointestinal symptoms may be associated with the type of carbohydrates consumed.					
29311037	4	62	theme	Medical	573:579	arg1	QOL					569:571	QOL Medical	569:579	QOL Medical	569:579	OBJECTIVE To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms.					
29311037	14	63	theme	%	1739:1739	arg1	fiber					1741:1745	8% fiber	1738:1745	8% fiber	1738:1745	Average carbohydrate composition was 35% starch, 8% fiber, and 59% sugars.					
29311037	0	64	theme	Enzyme	99:104	arg1	Treatment					106:114	Enzyme Treatment	99:114	Enzyme Treatment	99:114	Relationships among Dietary Intakes and Persistent Gastrointestinal Symptoms in Patients Receiving Enzyme Treatment for Genetic Sucrase-Isomaltase Deficiency.					
29311037	2	65	from	disaccharides	350:362	arg1	diarrhea					273:280	diarrhea	273:280	diarrhea	273:280	Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides.					
29311037	2	65	from	disaccharides	350:362	arg1	bloating					293:300	abdominal bloating	283:300	abdominal bloating	283:300	Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides.					
29311037	2	65	from	disaccharides	350:362	arg1	flatulence					307:316	flatulence	307:316	flatulence	307:316	Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides.					
29311037	18	66	theme	CONCLUSIONS	1943:1953	arg1	Intakes					1955:1961	CONCLUSIONS Intakes	1943:1961	CONCLUSIONS Intakes	1943:1961	CONCLUSIONS Intakes were lower in carbohydrates and higher in fat compared with the Acceptable Macronutrient Distribution Ranges.					
29311037	7	67	theme	symptoms	1095:1102	arg1	interviews					1035:1044	24-hour diet recall interviews	1015:1044	24-hour diet recall interviews	1015:1044	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	7	67	theme	symptoms	1095:1102	arg1	dose					1121:1124	sacrosidase dose	1109:1124	sacrosidase dose	1109:1124	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	7	67	theme	symptoms	1095:1102	arg1	severity					1061:1068	severity	1061:1068	severity	1061:1068	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	7	67	theme	symptoms	1095:1102	arg1	frequency					1047:1055	frequency	1047:1055	frequency	1047:1055	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	20	68	theme	symptom	2199:2205	arg1	frequency					2207:2215	GI symptom frequency	2196:2215	GI symptom frequency	2196:2215	Higher maltose and lactose intakes were associated with GI symptom frequency and severity.					
29311037	12	69	from	1,562.5±411.5	1497:1509	arg1	children					1523:1530	children	1523:1530	children	1523:1530	Energy intakes averaged 1,562.5±411.5 kcal/day in children, 1,964.7±823.6 kcal/day in adolescents, and 1,952.6±546.5 kcal/day in adults.					
29311037	11	70	theme	higher	1446:1451	arg1	intake					1465:1470	higher sacrosidase intake	1446:1470	higher sacrosidase intake	1446:1470	Having less frequent GI symptoms was associated with higher sacrosidase intake.					
29311037	0	71	theme	Sucrase-Isomaltase	128:145	arg1	Deficiency					147:156	Genetic Sucrase-Isomaltase Deficiency	120:156	Genetic Sucrase-Isomaltase Deficiency	120:156	Relationships among Dietary Intakes and Persistent Gastrointestinal Symptoms in Patients Receiving Enzyme Treatment for Genetic Sucrase-Isomaltase Deficiency.					
29311037	8	72	theme	sacrosidase	1198:1208	arg1	dose					1210:1213	sacrosidase dose	1198:1213	sacrosidase dose	1198:1213	STATISTICAL ANALYSES PERFORMED Relationships between nutrient intakes, sacrosidase dose, and GI symptoms were evaluated using Spearman ρ correlation coefficients.					
29311037	12	73	theme	Energy	1473:1478	arg1	intakes					1480:1486	Energy intakes	1473:1486	Energy intakes	1473:1486	Energy intakes averaged 1,562.5±411.5 kcal/day in children, 1,964.7±823.6 kcal/day in adolescents, and 1,952.6±546.5 kcal/day in adults.					
29311037	13	74	theme	%	1678:1678	arg1	protein					1680:1686	17% protein	1676:1686	17% protein	1676:1686	Macronutrient composition averaged 44% carbohydrate, 39% fat, and 17% protein.					
29311037	7	75	theme	gastrointestinal	1073:1088	arg1	GI					1091:1092	GI	1091:1092	GI	1091:1092	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	7	75	theme	gastrointestinal	1073:1088	arg1	symptoms					1095:1102	gastrointestinal (GI) symptoms	1073:1102	gastrointestinal (GI) symptoms	1073:1102	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	3	76	theme	carbohydrates	452:464	arg1	type					444:447	the type	440:447	the type of carbohydrates consumed	440:473	Frequency and severity of gastrointestinal symptoms may be associated with the type of carbohydrates consumed.					
29311037	14	77	theme	carbohydrate	1697:1708	arg1	composition					1710:1720	Average carbohydrate composition	1689:1720	Average carbohydrate composition	1689:1720	Average carbohydrate composition was 35% starch, 8% fiber, and 59% sugars.					
29311037	12	78	dep	1,562.5±411.5	1497:1509	arg1	kcal/day					1511:1518	kcal/day	1511:1518	1,562.5±411.5 kcal/day in children	1497:1530	Energy intakes averaged 1,562.5±411.5 kcal/day in children, 1,964.7±823.6 kcal/day in adolescents, and 1,952.6±546.5 kcal/day in adults.					
29311037	4	79	dep	Sucraid	560:566	arg1	QOL					569:571	QOL Medical	569:579	QOL Medical	569:579	OBJECTIVE To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms.					
29311037	8	80	theme	GI	1220:1221	arg1	symptoms					1223:1230	GI symptoms	1220:1230	GI symptoms	1220:1230	STATISTICAL ANALYSES PERFORMED Relationships between nutrient intakes, sacrosidase dose, and GI symptoms were evaluated using Spearman ρ correlation coefficients.					
29311037	4	81	theme	carbohydrates	634:646	arg1	dose					661:664	sacrosidase dose	649:664	sacrosidase dose	649:664	OBJECTIVE To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms.					
29311037	4	81	theme	carbohydrates	634:646	arg1	type					626:629	type	626:629	type of carbohydrates	626:646	OBJECTIVE To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms.					
29311037	4	81	theme	carbohydrates	634:646	arg1	symptoms					688:695	gastrointestinal symptoms	671:695	gastrointestinal symptoms	671:695	OBJECTIVE To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms.					
29311037	11	82	theme	frequent	1405:1412	arg1	symptoms					1417:1424	less frequent GI symptoms	1400:1424	less frequent GI symptoms	1400:1424	Having less frequent GI symptoms was associated with higher sacrosidase intake.					
29311037	2	83	theme	Absent	230:235	arg1	activity					255:262	Absent or reduced enzyme activity	230:262	Absent or reduced enzyme activity	230:262	Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides.					
29311037	0	84	theme	Persistent	40:49	arg1	Symptoms					68:75	Persistent Gastrointestinal Symptoms	40:75	Persistent Gastrointestinal Symptoms	40:75	Relationships among Dietary Intakes and Persistent Gastrointestinal Symptoms in Patients Receiving Enzyme Treatment for Genetic Sucrase-Isomaltase Deficiency.					
29311037	6	85	theme	nationwide	873:882	arg1	database					893:900	the enzyme manufacturer's nationwide clinical database	847:900	the enzyme manufacturer's nationwide clinical database between November 2014 and August 2015	847:938	PARTICIPANTS/SETTING Forty-nine patients treated with sacrosidase for ≥3 months were recruited from the enzyme manufacturer's nationwide clinical database between November 2014 and August 2015.					
29311037	8	86	theme	ρ	1262:1262	arg1	coefficients					1276:1287	Spearman ρ correlation coefficients	1253:1287	Spearman ρ correlation coefficients	1253:1287	STATISTICAL ANALYSES PERFORMED Relationships between nutrient intakes, sacrosidase dose, and GI symptoms were evaluated using Spearman ρ correlation coefficients.					
29311037	4	87	theme	gastrointestinal	671:686	arg1	symptoms					688:695	gastrointestinal symptoms	671:695	gastrointestinal symptoms	671:695	OBJECTIVE To characterize the dietary intakes of patients treated with sacrosidase (Sucraid; QOL Medical) for SID and determine relationships between type of carbohydrates, sacrosidase dose, and gastrointestinal symptoms.					
29311037	2	88	theme	reduced	240:246	arg1	activity					255:262	Absent or reduced enzyme activity	230:262	Absent or reduced enzyme activity	230:262	Absent or reduced enzyme activity promotes diarrhea, abdominal bloating, and flatulence from undigested and malabsorbed disaccharides.					
29311037	9	89	theme	Sacrosidase	1298:1308	arg1	dose					1310:1313	RESULTS Sacrosidase dose	1290:1313	RESULTS Sacrosidase dose	1290:1313	RESULTS Sacrosidase dose averaged 5.2±3.1 mL/day.					
29311037	13	90	theme	39	1663:1664	arg1	%					1665:1665	%	1665:1665	%	1665:1665	Macronutrient composition averaged 44% carbohydrate, 39% fat, and 17% protein.					
29311037	7	91	theme	recall	1028:1033	arg1	interviews					1035:1044	24-hour diet recall interviews	1015:1044	24-hour diet recall interviews	1015:1044	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	7	92	dep	MEASURES	954:961	arg1	energy					971:976	Dietary energy and nutrient intakes	963:997	energy	971:976	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	7	92	dep	MEASURES	954:961	arg1	intakes					991:997	Dietary energy and nutrient intakes	963:997	intakes	991:997	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29311037	7	93	theme	24-hour	1015:1021	arg1	interviews					1035:1044	24-hour diet recall interviews	1015:1044	24-hour diet recall interviews	1015:1044	MAIN OUTCOME MEASURES Dietary energy and nutrient intakes reported during 24-hour diet recall interviews, frequency and severity of gastrointestinal (GI) symptoms, and sacrosidase dose.					
29698465	4	0	theme	LBG	738:740	arg1	BG					734:735	BG	734:735	low BG (LBG; 0.6% BG) levels	730:757	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	4	0	theme	LBG	738:740	arg1	BG					748:749	LBG; 0.6% BG	738:749	LBG; 0.6% BG	738:749	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	3	1	theme	study	471:475	arg1	objective					453:461	The objective	449:461	The objective of this study	449:475	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	11	2	theme	sensitivity	1782:1792	arg1	improvement					1759:1769	improvement	1759:1769	improvement of insulin sensitivity	1759:1792	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	11	2	theme	sensitivity	1782:1792	arg1	appetite					1746:1753	appetite	1746:1753	appetite	1746:1753	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	5	3	with	BG	920:921	arg1	cellulose					869:877	5% cellulose	866:877	5% cellulose	866:877	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	11	4	theme	BG	1705:1706	arg1	effects					1687:1693	the beneficial metabolic effects	1662:1693	the beneficial metabolic effects of barley BG	1662:1706	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	11	4	theme	BG	1705:1706	arg1	due					1720:1722	due	1720:1722	due	1720:1722	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	11	5	theme	gut	1816:1818	arg1	secretion					1828:1836	gut hormone secretion	1816:1836	gut hormone secretion promoted via gut microbiota-produced SCFAs	1816:1879	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	6	6	theme	microbial	994:1002	arg1	compositions					1004:1015	gut microbial compositions	990:1015	gut microbial compositions	990:1015	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	11	7	theme	gut	1851:1853	arg1	SCFAs					1875:1879	gut microbiota-produced SCFAs	1851:1879	gut microbiota-produced SCFAs	1851:1879	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	1	8	theme	β-glucan	164:171	arg1	intake					147:152	Dietary intake	139:152	Dietary intake of barley β-glucan (BG)	139:176	Dietary intake of barley β-glucan (BG) is known to affect energy metabolism.					
29698465	7	9	theme	control	1163:1169	arg1	mice					1171:1174	control mice	1163:1174	control mice	1163:1174	The weight gain and fat mass of HBG-fed mice were lower than those of control mice at 16-wk-old.					
29698465	4	10	theme	BG	734:735	arg1	levels					752:757	low BG (LBG; 0.6% BG) levels	730:757	low BG (LBG; 0.6% BG) levels	730:757	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	7	11	theme	HBG-fed	1125:1131	arg1	mice					1133:1136	HBG-fed mice	1125:1136	HBG-fed mice	1125:1136	The weight gain and fat mass of HBG-fed mice were lower than those of control mice at 16-wk-old.					
29698465	11	12	theme	insulin	1774:1780	arg1	sensitivity					1782:1792	insulin sensitivity	1774:1792	insulin sensitivity	1774:1792	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	4	13	theme	conventional	765:776	arg1	conditions					797:806	conventional and germ-free (GF) conditions	765:806	conventional and germ-free (GF) conditions	765:806	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	8	14	dep	improving	1308:1316	arg1	butyrate					1403:1410	butyrate	1403:1410	butyrate	1403:1410	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	8	14	dep	improving	1308:1316	arg1	especially					1391:1400	especially	1391:1400	especially	1391:1400	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	0	15	theme	fat	120:122	arg1	diet					124:127	high fat diet	115:127	high fat diet fed mice	115:136	Barley β-glucan improves metabolic condition via short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice.					
29698465	4	16	theme	germ-free	782:790	arg1	conditions					797:806	conventional and germ-free (GF) conditions	765:806	conventional and germ-free (GF) conditions	765:806	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	4	17	contain	containing	688:697	arg1	flour					682:686	20% barley flour	671:686	20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions	671:806	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	4	17	contain	containing	688:697	arg2	levels					752:757	low BG (LBG; 0.6% BG) levels	730:757	low BG (LBG; 0.6% BG) levels	730:757	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	4	17	contain	containing	688:697	arg2	BG					711:712	high BG	706:712	high BG (HBG; 2% BG)	706:725	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	6	18	theme	acids	1064:1068	arg1	production					1026:1035	the production	1022:1035	the production of fecal short-chain fatty acids (SCFAs)	1022:1076	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	6	18	theme	acids	1064:1068	arg1	compositions					1004:1015	gut microbial compositions	990:1015	gut microbial compositions	990:1015	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	6	18	theme	acids	1064:1068	arg1	parameters					978:987	metabolic parameters	968:987	metabolic parameters	968:987	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	7	19	theme	weight	1097:1102	arg1	gain					1104:1107	weight gain	1097:1107	weight gain	1097:1107	The weight gain and fat mass of HBG-fed mice were lower than those of control mice at 16-wk-old.					
29698465	6	20	theme	short-chain	1046:1056	arg1	SCFAs					1071:1075	SCFAs	1071:1075	SCFAs	1071:1075	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	6	20	theme	short-chain	1046:1056	arg1	acids					1064:1068	fecal short-chain fatty acids	1040:1068	fecal short-chain fatty acids (SCFAs)	1040:1076	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	3	21	theme	barley	552:557	arg1	BG					559:560	barley BG	552:560	barley BG	552:560	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	11	22	theme	beneficial	1666:1675	arg1	effects					1687:1693	the beneficial metabolic effects	1662:1693	the beneficial metabolic effects of barley BG	1662:1706	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	11	22	theme	beneficial	1666:1675	arg1	due					1720:1722	due	1720:1722	due	1720:1722	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	5	23	theme	5	866:866	arg1	%					867:867	%	867:867	%	867:867	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	11	24	theme	barley	1698:1703	arg1	BG					1705:1706	barley BG	1698:1706	barley BG	1698:1706	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	4	25	theme	Male	622:625	arg1	mice					645:648	Male 4-wk-old C57BL/6J mice	622:648	Male 4-wk-old C57BL/6J mice	622:648	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	3	26	theme	mouse	570:574	arg1	model					576:580	a mouse model	568:580	a mouse model of high fat diet (HFD)-induced obesity	568:619	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	1	27	theme	Dietary	139:145	arg1	intake					147:152	Dietary intake	139:152	Dietary intake of barley β-glucan (BG)	139:176	Dietary intake of barley β-glucan (BG) is known to affect energy metabolism.					
29698465	9	28	theme	HBG-fed	1460:1466	arg1	mice					1468:1471	HBG-fed mice	1460:1471	HBG-fed mice	1460:1471	These effects in HBG-fed mice were abolished under GF conditions.					
29698465	4	29	theme	C57BL/6J	636:643	arg1	mice					645:648	Male 4-wk-old C57BL/6J mice	622:648	Male 4-wk-old C57BL/6J mice	622:648	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	5	30	theme	%	911:911	arg1	HFB					924:926	HFB	924:926	HFB; high fiber β-glucan	924:947	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	5	30	theme	%	911:911	arg1	BG					920:921	5% barley BG	910:921	5% barley BG (HFB; high fiber β-glucan)	910:948	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	11	31	theme	metabolic	1677:1685	arg1	effects					1687:1693	the beneficial metabolic effects	1662:1693	the beneficial metabolic effects of barley BG	1662:1706	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	11	31	theme	metabolic	1677:1685	arg1	due					1720:1722	due	1720:1722	due	1720:1722	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	0	32	theme	microbial	89:97	arg1	fermentation					99:110	gut microbial fermentation	85:110	gut microbial fermentation	85:110	Barley β-glucan improves metabolic condition via short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice.					
29698465	5	33	theme	high	885:888	arg1	cellulose					896:904	high fiber cellulose	885:904	HFC; high fiber cellulose	880:904	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	8	34	theme	gut	1221:1223	arg1	hormones					1225:1232	the gut hormones PYY and GLP-1	1217:1246	the gut hormones PYY and GLP-1	1217:1246	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	8	34	theme	gut	1221:1223	arg1	GLP-1					1242:1246	GLP-1	1242:1246	GLP-1	1242:1246	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	8	34	theme	gut	1221:1223	arg1	PYY					1234:1236	PYY	1234:1236	PYY	1234:1236	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	2	35	theme	loss	421:424	arg1	terms					381:385	terms	381:385	terms of satiety, energy intake, weight loss, and glycemic control	381:446	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	0	36	theme	Barley	0:5	arg1	β-glucan					7:14	Barley β-glucan	0:14	Barley β-glucan	0:14	Barley β-glucan improves metabolic condition via short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice.					
29698465	5	37	theme	high	929:932	arg1	β-glucan					940:947	high fiber β-glucan	929:947	HFB; high fiber β-glucan	924:947	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	4	38	theme	%	673:673	arg1	flour					682:686	20% barley flour	671:686	20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions	671:806	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	2	39	theme	energy	399:404	arg1	intake					406:411	energy intake	399:411	energy intake	399:411	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	2	40	theme	control	440:446	arg1	terms					381:385	terms	381:385	terms of satiety, energy intake, weight loss, and glycemic control	381:446	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	4	41	theme	2	720:720	arg1	%					721:721	%	721:721	%	721:721	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	2	42	theme	satiety	390:396	arg1	terms					381:385	terms	381:385	terms of satiety, energy intake, weight loss, and glycemic control	381:446	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	1	43	theme	barley	157:162	arg1	BG					174:175	BG	174:175	BG	174:175	Dietary intake of barley β-glucan (BG) is known to affect energy metabolism.					
29698465	1	43	theme	barley	157:162	arg1	β-glucan					164:171	barley β-glucan	157:171	barley β-glucan (BG)	157:176	Dietary intake of barley β-glucan (BG) is known to affect energy metabolism.					
29698465	11	44	theme	microbiota-produced	1855:1873	arg1	SCFAs					1875:1879	gut microbiota-produced SCFAs	1851:1879	gut microbiota-produced SCFAs	1851:1879	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	3	45	theme	fat	590:592	arg1	diet					594:597	high fat diet	585:597	high fat diet (HFD)	585:603	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	3	45	theme	fat	590:592	arg1	HFD					600:602	HFD	600:602	HFD	600:602	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	0	46	theme	short-chain	49:59	arg1	acids					67:71	short-chain fatty acids	49:71	short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice	49:136	Barley β-glucan improves metabolic condition via short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice.					
29698465	10	47	theme	PYY	1548:1550	arg1	secretion					1562:1570	PYY and GLP-1 secretion	1548:1570	PYY and GLP-1 secretion	1548:1570	Moreover, the HFB diets also increased PYY and GLP-1 secretion, and decreased food intake compared with that in HFC-fed mice.					
29698465	3	48	theme	obesity	613:619	arg1	model					576:580	a mouse model	568:580	a mouse model of high fat diet (HFD)-induced obesity	568:619	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	9	49	from	effects	1449:1455	arg1	mice					1468:1471	HBG-fed mice	1460:1471	HBG-fed mice	1460:1471	These effects in HBG-fed mice were abolished under GF conditions.					
29698465	8	50	theme	food	1292:1295	arg1	intake					1297:1302	food intake	1292:1302	food intake	1292:1302	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	2	51	theme	negative	352:359	arg1	effects					361:367	both positive and negative effects	334:367	both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control	334:446	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	7	52	dep	gain	1104:1107	arg1	The					1093:1095	The	1093:1095	The	1093:1095	The weight gain and fat mass of HBG-fed mice were lower than those of control mice at 16-wk-old.					
29698465	8	53	dep	hormones	1225:1232	arg1	hormones					1225:1232	the gut hormones PYY and GLP-1	1217:1246	the gut hormones PYY and GLP-1	1217:1246	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	8	53	dep	hormones	1225:1232	arg1	GLP-1					1242:1246	GLP-1	1242:1246	GLP-1	1242:1246	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	8	53	dep	hormones	1225:1232	arg1	PYY					1234:1236	PYY	1234:1236	PYY	1234:1236	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	2	54	theme	positive	339:346	arg1	effects					361:367	both positive and negative effects	334:367	both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control	334:446	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	8	55	theme	insulin	1318:1324	arg1	sensitivity					1326:1336	insulin sensitivity	1318:1336	insulin sensitivity	1318:1336	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	10	56	theme	food	1587:1590	arg1	intake					1592:1597	food intake	1587:1597	food intake	1587:1597	Moreover, the HFB diets also increased PYY and GLP-1 secretion, and decreased food intake compared with that in HFC-fed mice.					
29698465	3	57	theme	physiological	496:508	arg1	role					510:513	the physiological role	492:513	the physiological role underlying the metabolic benefits of barley BG	492:560	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	10	58	theme	HFC-fed	1621:1627	arg1	mice					1629:1632	HFC-fed mice	1621:1632	HFC-fed mice	1621:1632	Moreover, the HFB diets also increased PYY and GLP-1 secretion, and decreased food intake compared with that in HFC-fed mice.					
29698465	4	59	dep	HBG	715:717	arg1	BG					723:724	2% BG	720:724	HBG; 2% BG	715:724	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	11	60	theme	hormone	1820:1826	arg1	secretion					1828:1836	gut hormone secretion	1816:1836	gut hormone secretion promoted via gut microbiota-produced SCFAs	1816:1879	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	6	61	theme	gut	990:992	arg1	compositions					1004:1015	gut microbial compositions	990:1015	gut microbial compositions	990:1015	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	3	62	theme	metabolic	530:538	arg1	benefits					540:547	the metabolic benefits	526:547	the metabolic benefits of barley BG	526:560	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	4	63	dep	BG	711:712	arg1	HBG					715:717	HBG	715:717	HBG; 2% BG	715:724	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	4	64	theme	low	730:732	arg1	levels					752:757	low BG (LBG; 0.6% BG) levels	730:757	low BG (LBG; 0.6% BG) levels	730:757	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	7	65	theme	mice	1133:1136	arg1	gain					1104:1107	weight gain	1097:1107	weight gain	1097:1107	The weight gain and fat mass of HBG-fed mice were lower than those of control mice at 16-wk-old.					
29698465	7	65	theme	mice	1133:1136	arg1	mass					1117:1120	fat mass	1113:1120	fat mass	1113:1120	The weight gain and fat mass of HBG-fed mice were lower than those of control mice at 16-wk-old.					
29698465	8	66	theme	conventional	1419:1430	arg1	condition					1432:1440	conventional condition	1419:1440	conventional condition	1419:1440	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	11	67	theme	appetite	1746:1753	arg1	suppression					1731:1741	the suppression	1727:1741	the suppression	1727:1741	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	3	68	theme	BG	559:560	arg1	benefits					540:547	the metabolic benefits	526:547	the metabolic benefits of barley BG	526:560	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	6	69	theme	metabolic	968:976	arg1	parameters					978:987	metabolic parameters	968:987	metabolic parameters	968:987	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	6	70	theme	fatty	1058:1062	arg1	SCFAs					1071:1075	SCFAs	1071:1075	SCFAs	1071:1075	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	6	70	theme	fatty	1058:1062	arg1	acids					1064:1068	fecal short-chain fatty acids	1040:1068	fecal short-chain fatty acids (SCFAs)	1040:1076	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	4	71	theme	%	746:746	arg1	BG					734:735	BG	734:735	low BG (LBG; 0.6% BG) levels	730:757	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	4	71	theme	%	746:746	arg1	BG					748:749	LBG; 0.6% BG	738:749	LBG; 0.6% BG	738:749	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	6	72	theme	fecal	1040:1044	arg1	SCFAs					1071:1075	SCFAs	1071:1075	SCFAs	1071:1075	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	6	72	theme	fecal	1040:1044	arg1	acids					1064:1068	fecal short-chain fatty acids	1040:1068	fecal short-chain fatty acids (SCFAs)	1040:1076	Then, metabolic parameters, gut microbial compositions, and the production of fecal short-chain fatty acids (SCFAs) were analyzed.					
29698465	2	73	theme	underlying	229:238	arg1	mechanism					240:248	its underlying mechanism	225:248	its underlying mechanism	225:248	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	11	74	theme	improvement	1759:1769	arg1	suppression					1731:1741	the suppression	1727:1741	the suppression	1727:1741	These results suggest that the beneficial metabolic effects of barley BG are primary due to the suppression of appetite and improvement of insulin sensitivity, which are induced by gut hormone secretion promoted via gut microbiota-produced SCFAs.					
29698465	5	75	theme	%	867:867	arg1	cellulose					869:877	5% cellulose	866:877	5% cellulose	866:877	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	10	76	theme	HFB	1523:1525	arg1	diets					1527:1531	the HFB diets	1519:1531	the HFB diets	1519:1531	Moreover, the HFB diets also increased PYY and GLP-1 secretion, and decreased food intake compared with that in HFC-fed mice.					
29698465	0	77	theme	gut	85:87	arg1	fermentation					99:110	gut microbial fermentation	85:110	gut microbial fermentation	85:110	Barley β-glucan improves metabolic condition via short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice.					
29698465	5	78	theme	5	910:910	arg1	%					911:911	%	911:911	%	911:911	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	8	79	theme	hormones	1225:1232	arg1	secretion					1204:1212	the secretion	1200:1212	the secretion of the gut hormones PYY and GLP-1	1200:1246	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	4	80	theme	4-wk-old	627:634	arg1	mice					645:648	Male 4-wk-old C57BL/6J mice	622:648	Male 4-wk-old C57BL/6J mice	622:648	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	4	81	dep	germ-free	782:790	arg1	GF					793:794	GF	793:794	GF	793:794	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	5	82	theme	barley	913:918	arg1	HFB					924:926	HFB	924:926	HFB; high fiber β-glucan	924:947	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	5	82	theme	barley	913:918	arg1	BG					920:921	5% barley BG	910:921	5% barley BG (HFB; high fiber β-glucan)	910:948	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	5	83	dep	HFB	924:926	arg1	β-glucan					940:947	high fiber β-glucan	929:947	HFB; high fiber β-glucan	924:947	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	4	84	theme	barley	675:680	arg1	flour					682:686	20% barley flour	671:686	20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions	671:806	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	0	85	theme	high	115:118	arg1	diet					124:127	high fat diet	115:127	high fat diet fed mice	115:136	Barley β-glucan improves metabolic condition via short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice.					
29698465	5	86	theme	fiber	890:894	arg1	cellulose					896:904	high fiber cellulose	885:904	HFC; high fiber cellulose	880:904	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	9	87	theme	GF	1494:1495	arg1	conditions					1497:1506	GF conditions	1494:1506	GF conditions	1494:1506	These effects in HBG-fed mice were abolished under GF conditions.					
29698465	5	88	with	HFD	857:859	arg1	cellulose					869:877	5% cellulose	866:877	5% cellulose	866:877	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	2	89	theme	weight	414:419	arg1	loss					421:424	weight loss	414:424	weight loss	414:424	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	0	90	theme	metabolic	25:33	arg1	condition					35:43	metabolic condition	25:43	metabolic condition	25:43	Barley β-glucan improves metabolic condition via short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice.					
29698465	5	91	theme	fiber	934:938	arg1	β-glucan					940:947	high fiber β-glucan	929:947	HFB; high fiber β-glucan	924:947	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	2	92	theme	intake	406:411	arg1	terms					381:385	terms	381:385	terms of satiety, energy intake, weight loss, and glycemic control	381:446	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	8	93	theme	HBG-fed	1261:1267	arg1	mice					1269:1272	HBG-fed mice	1261:1272	HBG-fed mice	1261:1272	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	10	94	theme	GLP-1	1556:1560	arg1	secretion					1562:1570	PYY and GLP-1 secretion	1548:1570	PYY and GLP-1 secretion	1548:1570	Moreover, the HFB diets also increased PYY and GLP-1 secretion, and decreased food intake compared with that in HFC-fed mice.					
29698465	4	95	theme	20	671:672	arg1	%					673:673	%	673:673	%	673:673	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	2	96	theme	glycemic	431:438	arg1	control					440:446	glycemic control	431:446	glycemic control	431:446	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	3	97	theme	high	585:588	arg1	diet					594:597	high fat diet	585:597	high fat diet (HFD)	585:603	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	3	97	theme	high	585:588	arg1	HFD					600:602	HFD	600:602	HFD	600:602	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	0	98	theme	fatty	61:65	arg1	acids					67:71	short-chain fatty acids	49:71	short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice	49:136	Barley β-glucan improves metabolic condition via short-chain fatty acids produced by gut microbial fermentation in high fat diet fed mice.					
29698465	3	99	theme	-induced	604:611	arg1	obesity					613:619	high fat diet (HFD)-induced obesity	585:619	high fat diet (HFD)-induced obesity	585:619	The objective of this study was to clarify the physiological role underlying the metabolic benefits of barley BG using a mouse model of high fat diet (HFD)-induced obesity.					
29698465	4	100	theme	%	721:721	arg1	BG					723:724	2% BG	720:724	HBG; 2% BG	715:724	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	8	101	theme	gut	1354:1356	arg1	microbiota					1358:1367	the gut microbiota	1350:1367	the gut microbiota	1350:1367	Moreover, the secretion of the gut hormones PYY and GLP-1 increased in HBG-fed mice, thereby reducing food intake and improving insulin sensitivity by changing the gut microbiota and increasing SCFAs (especially, butyrate) under conventional condition.					
29698465	7	102	theme	fat	1113:1115	arg1	mass					1117:1120	fat mass	1113:1120	fat mass	1113:1120	The weight gain and fat mass of HBG-fed mice were lower than those of control mice at 16-wk-old.					
29698465	5	103	dep	HFC	880:882	arg1	cellulose					896:904	high fiber cellulose	885:904	HFC; high fiber cellulose	880:904	In addition, mice were fed either an HFD with 5% cellulose (HFC; high fiber cellulose) or 5% barley BG (HFB; high fiber β-glucan) for 12 wks.					
29698465	4	104	theme	high	706:709	arg1	BG					711:712	high BG	706:712	high BG (HBG; 2% BG)	706:725	Male 4-wk-old C57BL/6J mice were fed an HFD with 20% barley flour containing either high BG (HBG; 2% BG) or low BG (LBG; 0.6% BG) levels under conventional and germ-free (GF) conditions for 12 wks.					
29698465	2	105	theme	inconsistent	307:318	arg1	results					320:326	inconsistent results	307:326	inconsistent results	307:326	However, its underlying mechanism remains poorly understood because studies have presented inconsistent results, with both positive and negative effects reported in terms of satiety, energy intake, weight loss, and glycemic control.					
29698465	1	106	theme	energy	197:202	arg1	metabolism					204:213	energy metabolism	197:213	energy metabolism	197:213	Dietary intake of barley β-glucan (BG) is known to affect energy metabolism.					
31654769	5	0	theme	antioxidant	389:399	arg1	activities					401:410	antioxidant activities	389:410	antioxidant activities	389:410	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	7	1	theme	zebrafish	712:720	arg1	embryos					722:728	the LPS-induced zebrafish embryos	696:728	the LPS-induced zebrafish embryos	696:728	After that, SPEE exhibited significantly antioxidant properties in the LPS-induced zebrafish embryos, and the enzyme activities of ROS, CAT and SOD were obviously inhibited in a dose-dependent manner.					
31654769	8	2	theme	O	913:913	arg1	accumulation					895:906	lipid accumulation	889:906	lipid accumulation (Oil O staining)	889:923	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	8	2	theme	O	913:913	arg1	staining					915:922	Oil O staining	909:922	Oil O staining	909:922	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	5	3	theme	plebeia	468:474	arg1	extracts					453:460	ethanol extracts	445:460	ethanol extracts of S. plebeia (SPEE)	445:481	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	3	4	theme	inflammatory	204:215	arg1	hepatitis					234:242	hepatitis	234:242	hepatitis	234:242	is a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis.					
31654769	3	4	theme	inflammatory	204:215	arg1	diseases					217:224	many inflammatory diseases	199:224	many inflammatory diseases such as hepatitis	199:242	is a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis.					
31654769	17	5	from	effects	2280:2286	arg1	zebrafish					2291:2299	zebrafish	2291:2299	zebrafish	2291:2299	In summary, our results suggested that SPEE had an important role in the antioxidant and anti-inflammatory effects in zebrafish in the near future.					
31654769	17	5	from	effects	2280:2286	arg1	future					2313:2318	future	2313:2318	future	2313:2318	In summary, our results suggested that SPEE had an important role in the antioxidant and anti-inflammatory effects in zebrafish in the near future.					
31654769	18	6	theme	diseases	2472:2479	arg1	treatment					2446:2454	the treatment	2442:2454	the treatment of inflammatory diseases	2442:2479	Some of the components identified in this study may be served as potential sources of new hepatoprotective compounds for the treatment of inflammatory diseases.					
31654769	9	7	theme	SPEE	1142:1145	arg1	exposure					1147:1154	SPEE exposure	1142:1154	SPEE exposure	1142:1154	Meanwhile, the NO contents and lipid metabolism-related genes were substantially down-regulated after SPEE exposure.					
31654769	8	8	theme	hepatocyte	929:938	arg1	fibrosis					940:947	hepatocyte fibrosis	929:947	hepatocyte fibrosis (Gemori staining)	929:965	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	8	8	theme	hepatocyte	929:938	arg1	staining					957:964	Gemori staining	950:964	Gemori staining	950:964	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	5	9	theme	ethanol	445:451	arg1	extracts					453:460	ethanol extracts	445:460	ethanol extracts of S. plebeia (SPEE)	445:481	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	18	10	theme	potential	2386:2394	arg1	sources					2396:2402	potential sources	2386:2402	potential sources of new hepatoprotective compounds for the treatment of inflammatory diseases	2386:2479	Some of the components identified in this study may be served as potential sources of new hepatoprotective compounds for the treatment of inflammatory diseases.					
31654769	1	11	theme	Danio	76:80	arg1	zebrafish					65:73	zebrafish	65:73	zebrafish (Danio rerio)	65:87	extract in zebrafish (Danio rerio).					
31654769	1	11	theme	Danio	76:80	arg1	rerio					82:86	Danio rerio	76:86	Danio rerio	76:86	extract in zebrafish (Danio rerio).					
31654769	7	12	theme	antioxidant	670:680	arg1	properties					682:691	significantly antioxidant properties	656:691	significantly antioxidant properties	656:691	After that, SPEE exhibited significantly antioxidant properties in the LPS-induced zebrafish embryos, and the enzyme activities of ROS, CAT and SOD were obviously inhibited in a dose-dependent manner.					
31654769	14	13	theme	H2O2-stimulated	1861:1875	arg1	conditions					1877:1886	H2O2-stimulated conditions	1861:1886	H2O2-stimulated conditions	1861:1886	Moreover, SPEE can modulate various inflammatory cytokines and immune genes both in the control and H2O2-stimulated conditions.					
31654769	6	14	theme	chemical	534:541	arg1	compositions					543:554	the chemical compositions	530:554	the chemical compositions of SPEE	530:562	Firstly, we determined the chemical compositions of SPEE and identified three major constituents by using GC-MS analysis.					
31654769	12	15	theme	innate	1530:1535	arg1	immunity					1537:1544	innate immunity	1530:1544	innate immunity	1530:1544	KEGG pathway and GO functional analysis revealed that steroid biosynthesis, oxidation-reduction and innate immunity were significantly enriched.					
31654769	11	16	theme	TAA + SPEE	1403:1412	arg1	groups					1422:1427	the TAA + SPEE and TAA groups	1399:1427	groups	1422:1427	The results showed that 1289 DEGs including 558 up-regulated and 731 down-regulated were identified between the TAA + SPEE and TAA groups.					
31654769	13	17	from	nucleus	1710:1716	arg1	translocation					1671:1683	the translocation	1667:1683	the translocation of Nrf2 protein from the nucleus to the cytoplasm in TAA-induced zebrafish	1667:1758	Mechanistically, SPEE can considerably reduce the cell apoptosis of hepatocytes and promote the translocation of Nrf2 protein from the nucleus to the cytoplasm in TAA-induced zebrafish.					
31654769	5	18	theme	zebrafish	490:498	arg1	model					500:504	the zebrafish model	486:504	the zebrafish model	486:504	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	18	19	theme	compounds	2428:2436	arg1	sources					2396:2402	potential sources	2386:2402	potential sources of new hepatoprotective compounds for the treatment of inflammatory diseases	2386:2479	Some of the components identified in this study may be served as potential sources of new hepatoprotective compounds for the treatment of inflammatory diseases.					
31654769	14	20	theme	inflammatory	1797:1808	arg1	cytokines					1810:1818	various inflammatory cytokines	1789:1818	various inflammatory cytokines	1789:1818	Moreover, SPEE can modulate various inflammatory cytokines and immune genes both in the control and H2O2-stimulated conditions.					
31654769	13	21	theme	Nrf2	1688:1691	arg1	protein					1693:1699	Nrf2 protein	1688:1699	Nrf2 protein	1688:1699	Mechanistically, SPEE can considerably reduce the cell apoptosis of hepatocytes and promote the translocation of Nrf2 protein from the nucleus to the cytoplasm in TAA-induced zebrafish.					
31654769	5	22	from	activities	401:410	arg1	model					500:504	the zebrafish model	486:504	the zebrafish model	486:504	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	13	23	theme	hepatocytes	1643:1653	arg1	apoptosis					1630:1638	the cell apoptosis	1621:1638	the cell apoptosis of hepatocytes	1621:1653	Mechanistically, SPEE can considerably reduce the cell apoptosis of hepatocytes and promote the translocation of Nrf2 protein from the nucleus to the cytoplasm in TAA-induced zebrafish.					
31654769	3	24	theme	Chinese	129:135	arg1	herb					147:150	a traditional Chinese medicinal herb	115:150	a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis	115:242	is a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis.					
31654769	6	25	theme	GC-MS	613:617	arg1	analysis					619:626	GC-MS analysis	613:626	GC-MS analysis	613:626	Firstly, we determined the chemical compositions of SPEE and identified three major constituents by using GC-MS analysis.					
31654769	4	26	theme	underlying	258:267	arg1	mechanism					279:287	the underlying molecular mechanism	254:287	the underlying molecular mechanism about the hepatoprotective effects of S. plebeia	254:336	However, the underlying molecular mechanism about the hepatoprotective effects of S. plebeia remains largely unknown.					
31654769	10	27	from	exposure	1256:1263	arg1	liver					1284:1288	adult zebrafish liver	1268:1288	adult zebrafish liver	1268:1288	Thirdly, we used RNA-Seq analysis to identify the differentially expressed genes (DEGs) after SPEE exposure in adult zebrafish liver.					
31654769	5	28	dep	activities	401:410	arg1	the					385:387	the	385:387	the	385:387	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	14	29	theme	various	1789:1795	arg1	cytokines					1810:1818	various inflammatory cytokines	1789:1818	various inflammatory cytokines	1789:1818	Moreover, SPEE can modulate various inflammatory cytokines and immune genes both in the control and H2O2-stimulated conditions.					
31654769	15	30	theme	SPEE	2053:2056	arg1	treatment					2058:2066	SPEE treatment	2053:2066	SPEE treatment	2053:2066	The pro-inflammatory cytokines such as IL-1β and TNF-α was markedly up-regulated but the anti-inflammatory cytokines such as TGF-β was greatly down-regulated after SPEE treatment.					
31654769	8	31	theme	fat	861:863	arg1	vacuoles					865:872	fat vacuoles	861:872	fat vacuoles (HE staining)	861:886	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	8	31	theme	fat	861:863	arg1	staining					878:885	HE staining	875:885	HE staining	875:885	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	8	32	theme	-induced	993:1000	arg1	injury					1013:1018	the thioacetamide (TAA)-induced hepatocyte injury	970:1018	the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish	970:1037	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	18	33	theme	hepatoprotective	2411:2426	arg1	compounds					2428:2436	new hepatoprotective compounds	2407:2436	new hepatoprotective compounds	2407:2436	Some of the components identified in this study may be served as potential sources of new hepatoprotective compounds for the treatment of inflammatory diseases.					
31654769	0	34	theme	Salvia	32:37	arg1	plebeia					39:45	Salvia plebeia	32:45	Salvia plebeia	32:45	The hepatoprotective effects of Salvia plebeia R. Br.					
31654769	12	35	theme	functional	1450:1459	arg1	analysis					1461:1468	functional analysis	1450:1468	functional analysis	1450:1468	KEGG pathway and GO functional analysis revealed that steroid biosynthesis, oxidation-reduction and innate immunity were significantly enriched.					
31654769	15	36	theme	anti-inflammatory	1978:1994	arg1	cytokines					1996:2004	the anti-inflammatory cytokines	1974:2004	the anti-inflammatory cytokines such as TGF-β	1974:2018	The pro-inflammatory cytokines such as IL-1β and TNF-α was markedly up-regulated but the anti-inflammatory cytokines such as TGF-β was greatly down-regulated after SPEE treatment.					
31654769	15	36	theme	anti-inflammatory	1978:1994	arg1	TGF-β					2014:2018	TGF-β	2014:2018	TGF-β	2014:2018	The pro-inflammatory cytokines such as IL-1β and TNF-α was markedly up-regulated but the anti-inflammatory cytokines such as TGF-β was greatly down-regulated after SPEE treatment.					
31654769	10	37	theme	adult	1268:1272	arg1	liver					1284:1288	adult zebrafish liver	1268:1288	adult zebrafish liver	1268:1288	Thirdly, we used RNA-Seq analysis to identify the differentially expressed genes (DEGs) after SPEE exposure in adult zebrafish liver.					
31654769	7	38	theme	enzyme	739:744	arg1	activities					746:755	the enzyme activities	735:755	the enzyme activities of ROS, CAT and SOD	735:775	After that, SPEE exhibited significantly antioxidant properties in the LPS-induced zebrafish embryos, and the enzyme activities of ROS, CAT and SOD were obviously inhibited in a dose-dependent manner.					
31654769	8	39	theme	adult	1023:1027	arg1	zebrafish					1029:1037	adult zebrafish	1023:1037	adult zebrafish	1023:1037	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	5	40	theme	anti-inflammatory	416:432	arg1	effects					434:440	anti-inflammatory effects	416:440	anti-inflammatory effects	416:440	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	12	41	theme	KEGG	1430:1433	arg1	pathway					1435:1441	KEGG pathway	1430:1441	KEGG pathway	1430:1441	KEGG pathway and GO functional analysis revealed that steroid biosynthesis, oxidation-reduction and innate immunity were significantly enriched.					
31654769	11	42	theme	1289	1315:1318	arg1	558					1335:1337	558	1335:1337	558	1335:1337	The results showed that 1289 DEGs including 558 up-regulated and 731 down-regulated were identified between the TAA + SPEE and TAA groups.					
31654769	11	42	theme	1289	1315:1318	arg1	731					1356:1358	731	1356:1358	731	1356:1358	The results showed that 1289 DEGs including 558 up-regulated and 731 down-regulated were identified between the TAA + SPEE and TAA groups.					
31654769	11	42	theme	1289	1315:1318	arg1	DEGs					1320:1323	1289 DEGs	1315:1323	1289 DEGs including 558 up-regulated and 731 down-regulated	1315:1373	The results showed that 1289 DEGs including 558 up-regulated and 731 down-regulated were identified between the TAA + SPEE and TAA groups.					
31654769	9	43	theme	lipid	1071:1075	arg1	genes					1096:1100	lipid metabolism-related genes	1071:1100	lipid metabolism-related genes	1071:1100	Meanwhile, the NO contents and lipid metabolism-related genes were substantially down-regulated after SPEE exposure.					
31654769	3	44	used	used	173:176	arg2	herb					147:150	a traditional Chinese medicinal herb	115:150	a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis	115:242	is a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis.					
31654769	8	45	theme	Oil	909:911	arg1	accumulation					895:906	lipid accumulation	889:906	lipid accumulation (Oil O staining)	889:923	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	8	45	theme	Oil	909:911	arg1	staining					915:922	Oil O staining	909:922	Oil O staining	909:922	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	18	46	theme	inflammatory	2459:2470	arg1	diseases					2472:2479	inflammatory diseases	2459:2479	inflammatory diseases	2459:2479	Some of the components identified in this study may be served as potential sources of new hepatoprotective compounds for the treatment of inflammatory diseases.					
31654769	7	47	theme	LPS-induced	700:710	arg1	embryos					722:728	the LPS-induced zebrafish embryos	696:728	the LPS-induced zebrafish embryos	696:728	After that, SPEE exhibited significantly antioxidant properties in the LPS-induced zebrafish embryos, and the enzyme activities of ROS, CAT and SOD were obviously inhibited in a dose-dependent manner.					
31654769	4	48	theme	plebeia	330:336	arg1	effects					316:322	the hepatoprotective effects	295:322	the hepatoprotective effects of S. plebeia	295:336	However, the underlying molecular mechanism about the hepatoprotective effects of S. plebeia remains largely unknown.					
31654769	1	49	from	extract	54:60	arg1	zebrafish					65:73	zebrafish	65:73	zebrafish (Danio rerio)	65:87	extract in zebrafish (Danio rerio).					
31654769	1	49	from	extract	54:60	arg1	rerio					82:86	Danio rerio	76:86	Danio rerio	76:86	extract in zebrafish (Danio rerio).					
31654769	4	50	theme	hepatoprotective	299:314	arg1	effects					316:322	the hepatoprotective effects	295:322	the hepatoprotective effects of S. plebeia	295:336	However, the underlying molecular mechanism about the hepatoprotective effects of S. plebeia remains largely unknown.					
31654769	3	51	theme	diseases	217:224	arg1	treatment					186:194	the treatment	182:194	the treatment of many inflammatory diseases such as hepatitis	182:242	is a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis.					
31654769	15	52	theme	pro-inflammatory	1893:1908	arg1	IL-1β					1928:1932	IL-1β	1928:1932	IL-1β	1928:1932	The pro-inflammatory cytokines such as IL-1β and TNF-α was markedly up-regulated but the anti-inflammatory cytokines such as TGF-β was greatly down-regulated after SPEE treatment.					
31654769	15	52	theme	pro-inflammatory	1893:1908	arg1	TNF-α					1938:1942	TNF-α	1938:1942	TNF-α	1938:1942	The pro-inflammatory cytokines such as IL-1β and TNF-α was markedly up-regulated but the anti-inflammatory cytokines such as TGF-β was greatly down-regulated after SPEE treatment.					
31654769	15	52	theme	pro-inflammatory	1893:1908	arg1	cytokines					1910:1918	The pro-inflammatory cytokines	1889:1918	The pro-inflammatory cytokines such as IL-1β and TNF-α	1889:1942	The pro-inflammatory cytokines such as IL-1β and TNF-α was markedly up-regulated but the anti-inflammatory cytokines such as TGF-β was greatly down-regulated after SPEE treatment.					
31654769	7	53	theme	dose-dependent	807:820	arg1	manner					822:827	a dose-dependent manner	805:827	a dose-dependent manner	805:827	After that, SPEE exhibited significantly antioxidant properties in the LPS-induced zebrafish embryos, and the enzyme activities of ROS, CAT and SOD were obviously inhibited in a dose-dependent manner.					
31654769	3	54	theme	many	199:202	arg1	hepatitis					234:242	hepatitis	234:242	hepatitis	234:242	is a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis.					
31654769	3	54	theme	many	199:202	arg1	diseases					217:224	many inflammatory diseases	199:224	many inflammatory diseases such as hepatitis	199:242	is a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis.					
31654769	8	55	theme	Gemori	950:955	arg1	fibrosis					940:947	hepatocyte fibrosis	929:947	hepatocyte fibrosis (Gemori staining)	929:965	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	8	55	theme	Gemori	950:955	arg1	staining					957:964	Gemori staining	950:964	Gemori staining	950:964	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	6	56	theme	SPEE	559:562	arg1	compositions					543:554	the chemical compositions	530:554	the chemical compositions of SPEE	530:562	Firstly, we determined the chemical compositions of SPEE and identified three major constituents by using GC-MS analysis.					
31654769	5	57	theme	extracts	453:460	arg1	activities					401:410	antioxidant activities	389:410	antioxidant activities	389:410	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	5	57	theme	extracts	453:460	arg1	effects					434:440	anti-inflammatory effects	416:440	anti-inflammatory effects	416:440	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	17	58	theme	anti-inflammatory	2262:2278	arg1	effects					2280:2286	the antioxidant and anti-inflammatory effects	2242:2286	the antioxidant and anti-inflammatory effects in zebrafish in the near future	2242:2318	In summary, our results suggested that SPEE had an important role in the antioxidant and anti-inflammatory effects in zebrafish in the near future.					
31654769	16	59	theme	H2O2-stimulated	2145:2159	arg1	conditions					2161:2170	the H2O2-stimulated conditions	2141:2170	the H2O2-stimulated conditions	2141:2170	In addition, some key genes in the TLR signaling were also activated in the H2O2-stimulated conditions.					
31654769	10	60	theme	RNA-Seq	1174:1180	arg1	analysis					1182:1189	RNA-Seq analysis	1174:1189	RNA-Seq analysis	1174:1189	Thirdly, we used RNA-Seq analysis to identify the differentially expressed genes (DEGs) after SPEE exposure in adult zebrafish liver.					
31654769	16	61	from	genes	2091:2095	arg1	signaling					2108:2116	the TLR signaling	2100:2116	the TLR signaling	2100:2116	In addition, some key genes in the TLR signaling were also activated in the H2O2-stimulated conditions.					
31654769	17	62	contain	had	2217:2219	arg2	role					2234:2237	an important role	2221:2237	an important role	2221:2237	In summary, our results suggested that SPEE had an important role in the antioxidant and anti-inflammatory effects in zebrafish in the near future.					
31654769	17	62	contain	had	2217:2219	arg1	SPEE					2212:2215	SPEE	2212:2215	SPEE	2212:2215	In summary, our results suggested that SPEE had an important role in the antioxidant and anti-inflammatory effects in zebrafish in the near future.					
31654769	11	63	theme	TAA	1418:1420	arg1	groups					1422:1427	the TAA + SPEE and TAA groups	1399:1427	groups	1422:1427	The results showed that 1289 DEGs including 558 up-regulated and 731 down-regulated were identified between the TAA + SPEE and TAA groups.					
31654769	14	64	theme	immune	1824:1829	arg1	genes					1831:1835	immune genes	1824:1835	immune genes	1824:1835	Moreover, SPEE can modulate various inflammatory cytokines and immune genes both in the control and H2O2-stimulated conditions.					
31654769	13	65	theme	TAA-induced	1738:1748	arg1	zebrafish					1750:1758	TAA-induced zebrafish	1738:1758	TAA-induced zebrafish	1738:1758	Mechanistically, SPEE can considerably reduce the cell apoptosis of hepatocytes and promote the translocation of Nrf2 protein from the nucleus to the cytoplasm in TAA-induced zebrafish.					
31654769	10	66	used	used	1169:1172	arg2	we					1166:1167	we	1166:1167	we	1166:1167	Thirdly, we used RNA-Seq analysis to identify the differentially expressed genes (DEGs) after SPEE exposure in adult zebrafish liver.					
31654769	17	67	theme	important	2224:2232	arg1	role					2234:2237	an important role	2221:2237	an important role	2221:2237	In summary, our results suggested that SPEE had an important role in the antioxidant and anti-inflammatory effects in zebrafish in the near future.					
31654769	3	68	theme	medicinal	137:145	arg1	herb					147:150	a traditional Chinese medicinal herb	115:150	a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis	115:242	is a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis.					
31654769	16	69	theme	key	2087:2089	arg1	genes					2091:2095	some key genes	2082:2095	some key genes in the TLR signaling	2082:2116	In addition, some key genes in the TLR signaling were also activated in the H2O2-stimulated conditions.					
31654769	6	70	theme	major	585:589	arg1	constituents					591:602	three major constituents	579:602	three major constituents	579:602	Firstly, we determined the chemical compositions of SPEE and identified three major constituents by using GC-MS analysis.					
31654769	12	71	theme	steroid	1484:1490	arg1	biosynthesis					1492:1503	steroid biosynthesis	1484:1503	steroid biosynthesis	1484:1503	KEGG pathway and GO functional analysis revealed that steroid biosynthesis, oxidation-reduction and innate immunity were significantly enriched.					
31654769	13	72	theme	protein	1693:1699	arg1	translocation					1671:1683	the translocation	1667:1683	the translocation of Nrf2 protein from the nucleus to the cytoplasm in TAA-induced zebrafish	1667:1758	Mechanistically, SPEE can considerably reduce the cell apoptosis of hepatocytes and promote the translocation of Nrf2 protein from the nucleus to the cytoplasm in TAA-induced zebrafish.					
31654769	3	73	theme	traditional	117:127	arg1	herb					147:150	a traditional Chinese medicinal herb	115:150	a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis	115:242	is a traditional Chinese medicinal herb that has been widely used for the treatment of many inflammatory diseases such as hepatitis.					
31654769	12	74	dep	pathway	1435:1441	arg1	analysis					1461:1468	functional analysis	1450:1468	functional analysis	1450:1468	KEGG pathway and GO functional analysis revealed that steroid biosynthesis, oxidation-reduction and innate immunity were significantly enriched.					
31654769	17	75	theme	antioxidant	2246:2256	arg1	effects					2280:2286	the antioxidant and anti-inflammatory effects	2242:2286	the antioxidant and anti-inflammatory effects in zebrafish in the near future	2242:2318	In summary, our results suggested that SPEE had an important role in the antioxidant and anti-inflammatory effects in zebrafish in the near future.					
31654769	10	76	theme	expressed	1222:1230	arg1	genes					1232:1236	the differentially expressed genes	1203:1236	the differentially expressed genes (DEGs)	1203:1243	Thirdly, we used RNA-Seq analysis to identify the differentially expressed genes (DEGs) after SPEE exposure in adult zebrafish liver.					
31654769	10	76	theme	expressed	1222:1230	arg1	DEGs					1239:1242	DEGs	1239:1242	DEGs	1239:1242	Thirdly, we used RNA-Seq analysis to identify the differentially expressed genes (DEGs) after SPEE exposure in adult zebrafish liver.					
31654769	14	77	dep	control	1849:1855	arg1	the					1845:1847	the	1845:1847	the	1845:1847	Moreover, SPEE can modulate various inflammatory cytokines and immune genes both in the control and H2O2-stimulated conditions.					
31654769	0	78	theme	hepatoprotective	4:19	arg1	effects					21:27	The hepatoprotective effects	0:27	The hepatoprotective effects of Salvia plebeia	0:45	The hepatoprotective effects of Salvia plebeia R. Br.					
31654769	13	79	from	cytoplasm	1725:1733	arg1	zebrafish					1750:1758	TAA-induced zebrafish	1738:1758	TAA-induced zebrafish	1738:1758	Mechanistically, SPEE can considerably reduce the cell apoptosis of hepatocytes and promote the translocation of Nrf2 protein from the nucleus to the cytoplasm in TAA-induced zebrafish.					
31654769	5	80	from	effects	434:440	arg1	model					500:504	the zebrafish model	486:504	the zebrafish model	486:504	Here, we investigated the antioxidant activities and anti-inflammatory effects of ethanol extracts of S. plebeia (SPEE) in the zebrafish model.					
31654769	16	81	theme	TLR	2104:2106	arg1	signaling					2108:2116	the TLR signaling	2100:2116	the TLR signaling	2100:2116	In addition, some key genes in the TLR signaling were also activated in the H2O2-stimulated conditions.					
31654769	10	82	theme	SPEE	1251:1254	arg1	exposure					1256:1263	SPEE exposure	1251:1263	SPEE exposure in adult zebrafish liver	1251:1288	Thirdly, we used RNA-Seq analysis to identify the differentially expressed genes (DEGs) after SPEE exposure in adult zebrafish liver.					
31654769	7	83	theme	SOD	773:775	arg1	activities					746:755	the enzyme activities	735:755	the enzyme activities of ROS, CAT and SOD	735:775	After that, SPEE exhibited significantly antioxidant properties in the LPS-induced zebrafish embryos, and the enzyme activities of ROS, CAT and SOD were obviously inhibited in a dose-dependent manner.					
31654769	8	84	theme	hepatocyte	1002:1011	arg1	injury					1013:1018	the thioacetamide (TAA)-induced hepatocyte injury	970:1018	the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish	970:1037	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	4	85	theme	molecular	269:277	arg1	mechanism					279:287	the underlying molecular mechanism	254:287	the underlying molecular mechanism about the hepatoprotective effects of S. plebeia	254:336	However, the underlying molecular mechanism about the hepatoprotective effects of S. plebeia remains largely unknown.					
31654769	13	86	theme	cell	1625:1628	arg1	apoptosis					1630:1638	the cell apoptosis	1621:1638	the cell apoptosis of hepatocytes	1621:1653	Mechanistically, SPEE can considerably reduce the cell apoptosis of hepatocytes and promote the translocation of Nrf2 protein from the nucleus to the cytoplasm in TAA-induced zebrafish.					
31654769	0	87	theme	plebeia	39:45	arg1	effects					21:27	The hepatoprotective effects	0:27	The hepatoprotective effects of Salvia plebeia	0:45	The hepatoprotective effects of Salvia plebeia R. Br.					
31654769	7	88	theme	CAT	765:767	arg1	activities					746:755	the enzyme activities	735:755	the enzyme activities of ROS, CAT and SOD	735:775	After that, SPEE exhibited significantly antioxidant properties in the LPS-induced zebrafish embryos, and the enzyme activities of ROS, CAT and SOD were obviously inhibited in a dose-dependent manner.					
31654769	0	89	dep	Br	50:51	arg1	effects					21:27	The hepatoprotective effects	0:27	The hepatoprotective effects of Salvia plebeia	0:45	The hepatoprotective effects of Salvia plebeia R. Br.					
31654769	18	90	theme	new	2407:2409	arg1	compounds					2428:2436	new hepatoprotective compounds	2407:2436	new hepatoprotective compounds	2407:2436	Some of the components identified in this study may be served as potential sources of new hepatoprotective compounds for the treatment of inflammatory diseases.					
31654769	10	91	theme	zebrafish	1274:1282	arg1	liver					1284:1288	adult zebrafish liver	1268:1288	adult zebrafish liver	1268:1288	Thirdly, we used RNA-Seq analysis to identify the differentially expressed genes (DEGs) after SPEE exposure in adult zebrafish liver.					
31654769	7	92	theme	ROS	760:762	arg1	activities					746:755	the enzyme activities	735:755	the enzyme activities of ROS, CAT and SOD	735:775	After that, SPEE exhibited significantly antioxidant properties in the LPS-induced zebrafish embryos, and the enzyme activities of ROS, CAT and SOD were obviously inhibited in a dose-dependent manner.					
31654769	8	93	theme	zebrafish	1029:1037	arg1	injury					1013:1018	the thioacetamide (TAA)-induced hepatocyte injury	970:1018	the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish	970:1037	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	8	94	theme	HE	875:876	arg1	vacuoles					865:872	fat vacuoles	861:872	fat vacuoles (HE staining)	861:886	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	8	94	theme	HE	875:876	arg1	staining					878:885	HE staining	875:885	HE staining	875:885	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	9	95	theme	NO	1055:1056	arg1	contents					1058:1065	the NO contents	1051:1065	the NO contents	1051:1065	Meanwhile, the NO contents and lipid metabolism-related genes were substantially down-regulated after SPEE exposure.					
31654769	8	96	theme	lipid	889:893	arg1	accumulation					895:906	lipid accumulation	889:906	lipid accumulation (Oil O staining)	889:923	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	8	96	theme	lipid	889:893	arg1	staining					915:922	Oil O staining	909:922	Oil O staining	909:922	Secondly, SPEE greatly reduced fat vacuoles (HE staining), lipid accumulation (Oil O staining) and hepatocyte fibrosis (Gemori staining) in the thioacetamide (TAA)-induced hepatocyte injury of adult zebrafish.					
31654769	9	97	theme	metabolism-related	1077:1094	arg1	genes					1096:1100	lipid metabolism-related genes	1071:1100	lipid metabolism-related genes	1071:1100	Meanwhile, the NO contents and lipid metabolism-related genes were substantially down-regulated after SPEE exposure.					
31740032	4	0	theme	reversed-phase	909:922	arg1	chromatography					924:937	the reversed-phase chromatography	905:937	the reversed-phase chromatography	905:937	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	3	1	theme	bridged	515:521	arg1	β-cyclodextrin					527:540	β-cyclodextrin	527:540	β-cyclodextrin	527:540	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	3	1	theme	bridged	515:521	arg1	bis					523:525	the bridged bis	511:525	the bridged bis(β-cyclodextrin)	511:541	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	7	2	theme	better	1464:1469	arg1	performance					1487:1497	a better chromatographic performance	1462:1497	a better chromatographic performance	1462:1497	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	8	3	theme	high	1694:1697	arg1	resolutions					1699:1709	high resolutions	1694:1709	high resolutions	1694:1709	For example, enantiomers of trimeprazine, praziquantel, flavanone and imazalil those could not be separated by CDCSP, were separated by SBCDP with high resolutions.					
31740032	3	4	theme	SBCDP	547:551	arg1	structures					497:506	The structures	493:506	The structures of the bridged bis(β-cyclodextrin) and SBCDP	493:551	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	1	5	theme	dicarboxylic	285:296	arg1	acid					298:301	4,4'-stilbene dicarboxylic acid	271:301	4,4'-stilbene dicarboxylic acid	271:301	A stilbene diamido-bridged bis(β-cyclodextrin) was synthesized via the reaction between 4,4'-stilbene dicarboxylic acid and 6-deoxy-6-amino-β-cyclodextrin.					
31740032	4	6	theme	triazole	882:889	arg1	pesticides					891:900	triazole pesticides	882:900	triazole pesticides	882:900	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	6	7	theme	high	1228:1231	arg1	1.51∼5.15					1246:1254	1.51∼5.15	1246:1254	1.51∼5.15	1246:1254	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	6	7	theme	high	1228:1231	arg1	resolutions					1233:1243	high resolutions	1228:1243	high resolutions (1.51∼5.15)	1228:1255	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	2	8	theme	chiral	460:465	arg1	SBCDP					485:489	SBCDP	485:489	SBCDP	485:489	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	2	8	theme	chiral	460:465	arg1	phase					478:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase	417:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP)	417:490	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	9	9	dep	analytes	1960:1967	arg1	complex					1941:1947	complex	1941:1947	complex	1941:1947	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	6	10	dep	5.15	1353:1356	arg1	to					1350:1351	to	1350:1351	to	1350:1351	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	6	11	theme	enantioseparation	1286:1302	arg1	resolutions					1304:1314	the enantioseparation resolutions	1282:1314	the enantioseparation resolutions of flavanone and imazalil	1282:1340	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	6	11	theme	enantioseparation	1286:1302	arg1	5.15					1353:1356	5.15	1353:1356	5.15	1353:1356	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	1	12	theme	diamido-bridged	194:208	arg1	β-cyclodextrin					214:227	β-cyclodextrin	214:227	β-cyclodextrin	214:227	A stilbene diamido-bridged bis(β-cyclodextrin) was synthesized via the reaction between 4,4'-stilbene dicarboxylic acid and 6-deoxy-6-amino-β-cyclodextrin.					
31740032	1	12	theme	diamido-bridged	194:208	arg1	bis					210:212	A stilbene diamido-bridged bis	183:212	A stilbene diamido-bridged bis(β-cyclodextrin)	183:228	A stilbene diamido-bridged bis(β-cyclodextrin) was synthesized via the reaction between 4,4'-stilbene dicarboxylic acid and 6-deoxy-6-amino-β-cyclodextrin.					
31740032	11	13	theme	chiral	2290:2295	arg1	compounds					2297:2305	chiral compounds	2290:2305	chiral compounds	2290:2305	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	0	14	theme	drugs	119:123	arg1	enantioseparations					97:114	enantioseparations	97:114	enantioseparations of drugs and pesticides	97:138	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	9	15	theme	native	1749:1754	arg1	CD					1756:1757	native CD	1749:1757	native CD	1749:1757	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	10	16	theme	temperature	2141:2151	arg1	range					2132:2136	a wide range	2125:2136	a wide range	2125:2136	Moreover, we also found that SBCDP possessed high enantioselectivity and diastereoselectivity over a wide range of temperature (30∼60 °C), which made the fast analysis possible.					
31740032	0	17	theme	pesticides	129:138	arg1	enantioseparations					97:114	enantioseparations	97:114	enantioseparations of drugs and pesticides	97:138	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	5	18	theme	column	1147:1152	arg1	temperature					1154:1164	column temperature	1147:1164	column temperature	1147:1164	The chromatographic conditions that affect the enantioselectivity or diasterioselectivity of SBCDP were investigated in detail, including the mobile phase composition, pH value and column temperature.					
31740032	3	19	theme	nuclear	598:604	arg1	resonance					615:623	nuclear magnetic resonance	598:623	nuclear magnetic resonance	598:623	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	4	20	theme	polar	946:950	arg1	mode					960:963	the polar organic mode	942:963	the polar organic mode	942:963	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	7	21	theme	stationary	1422:1431	arg1	phase					1433:1437	the native β-cyclodextrin stationary phase	1396:1437	the native β-cyclodextrin stationary phase (CDCSP)	1396:1445	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	7	21	theme	stationary	1422:1431	arg1	CDCSP					1440:1444	CDCSP	1440:1444	CDCSP	1440:1444	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	0	22	theme	high	143:146	arg1	chromatography					167:180	high performance liquid chromatography	143:180	high performance liquid chromatography	143:180	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	5	23	theme	phase	1115:1119	arg1	composition					1121:1131	the mobile phase composition	1104:1131	the mobile phase composition	1104:1131	The chromatographic conditions that affect the enantioselectivity or diasterioselectivity of SBCDP were investigated in detail, including the mobile phase composition, pH value and column temperature.					
31740032	9	24	theme	bridging	1764:1771	arg1	linker					1773:1778	the bridging linker	1760:1778	the bridging linker of the bridged bis(β-CD)	1760:1803	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	7	25	theme	native	1400:1405	arg1	phase					1433:1437	the native β-cyclodextrin stationary phase	1396:1437	the native β-cyclodextrin stationary phase (CDCSP)	1396:1445	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	7	25	theme	native	1400:1405	arg1	CDCSP					1440:1444	CDCSP	1440:1444	CDCSP	1440:1444	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	0	26	theme	liquid	160:165	arg1	chromatography					167:180	high performance liquid chromatography	143:180	high performance liquid chromatography	143:180	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	3	27	theme	infrared	626:633	arg1	spectroscopy					635:646	infrared spectroscopy	626:646	infrared spectroscopy	626:646	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	11	28	theme	new	2209:2211	arg1	material					2231:2238	a new chiral separation material	2207:2238	a new chiral separation material	2207:2238	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	11	28	theme	new	2209:2211	arg1	applications					2262:2273	wider applications	2256:2273	wider applications	2256:2273	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	4	29	theme	chromatographic	704:718	arg1	performance					720:730	The chromatographic performance	700:730	The chromatographic performance of SBCDP	700:739	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	8	30	dep	enantiomers	1560:1570	arg1	separated					1645:1653	separated	1645:1653	could not be separated by CDCSP	1632:1662	For example, enantiomers of trimeprazine, praziquantel, flavanone and imazalil those could not be separated by CDCSP, were separated by SBCDP with high resolutions.					
31740032	9	31	theme	bridged	1787:1793	arg1	β-CD					1799:1802	β-CD	1799:1802	β-CD	1799:1802	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	9	31	theme	bridged	1787:1793	arg1	bis					1795:1797	the bridged bis	1783:1797	the bridged bis(β-CD)	1783:1803	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	9	32	theme	SBCDP	2006:2010	arg1	discrimination					1988:2001	the chiral discrimination	1977:2001	the chiral discrimination of SBCDP more precise	1977:2023	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	0	33	theme	chiral	69:74	arg1	phase					87:91	chiral stationary phase	69:91	chiral stationary phase for enantioseparations of drugs and pesticides	69:138	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	9	34	theme	precise	2017:2023	arg1	SBCDP					2006:2010	SBCDP	2006:2010	SBCDP more precise	2006:2023	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	11	35	theme	separation	2220:2229	arg1	material					2231:2238	a new chiral separation material	2207:2238	a new chiral separation material	2207:2238	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	11	35	theme	separation	2220:2229	arg1	applications					2262:2273	wider applications	2256:2273	wider applications	2256:2273	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	2	36	theme	novel	419:423	arg1	SBCDP					485:489	SBCDP	485:489	SBCDP	485:489	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	2	36	theme	novel	419:423	arg1	phase					478:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase	417:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP)	417:490	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	5	37	theme	SBCDP	1059:1063	arg1	diasterioselectivity					1035:1054	diasterioselectivity	1035:1054	diasterioselectivity	1035:1054	The chromatographic conditions that affect the enantioselectivity or diasterioselectivity of SBCDP were investigated in detail, including the mobile phase composition, pH value and column temperature.					
31740032	5	37	theme	SBCDP	1059:1063	arg1	enantioselectivity					1013:1030	enantioselectivity	1013:1030	enantioselectivity	1013:1030	The chromatographic conditions that affect the enantioselectivity or diasterioselectivity of SBCDP were investigated in detail, including the mobile phase composition, pH value and column temperature.					
31740032	2	38	theme	stationary	467:476	arg1	SBCDP					485:489	SBCDP	485:489	SBCDP	485:489	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	2	38	theme	stationary	467:476	arg1	phase					478:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase	417:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP)	417:490	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	9	39	theme	small	1723:1727	arg1	cavity					1729:1734	the small cavity	1719:1734	the small cavity (0.65 nm) of native CD	1719:1757	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	9	39	theme	small	1723:1727	arg1	0.65 nm					1737:1743	0.65 nm	1737:1743	0.65 nm	1737:1743	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	2	40	theme	-bonded	452:458	arg1	SBCDP					485:489	SBCDP	485:489	SBCDP	485:489	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	2	40	theme	-bonded	452:458	arg1	phase					478:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase	417:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP)	417:490	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	4	41	theme	racemic	787:793	arg1	praziquantel					841:852	praziquantel	841:852	praziquantel	841:852	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	4	41	theme	racemic	787:793	arg1	drugs					795:799	23 racemic drugs	784:799	23 racemic drugs	784:799	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	4	41	theme	racemic	787:793	arg1	pesticides					891:900	triazole pesticides	882:900	triazole pesticides	882:900	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	4	41	theme	racemic	787:793	arg1	flavanones					855:864	flavanones	855:864	flavanones	855:864	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	4	41	theme	racemic	787:793	arg1	β-blockers					867:876	β-blockers	867:876	β-blockers	867:876	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	4	41	theme	racemic	787:793	arg1	trimeprazine					827:838	trimeprazine	827:838	trimeprazine	827:838	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	2	42	theme	β-cyclodextrin	437:450	arg1	SBCDP					485:489	SBCDP	485:489	SBCDP	485:489	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	2	42	theme	β-cyclodextrin	437:450	arg1	phase					478:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase	417:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP)	417:490	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	2	43	theme	bis	433:435	arg1	SBCDP					485:489	SBCDP	485:489	SBCDP	485:489	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	2	43	theme	bis	433:435	arg1	phase					478:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase	417:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP)	417:490	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	0	44	theme	diamido-bridged	26:40	arg1	bis					42:44	a stilbene diamido-bridged bis	15:44	a stilbene diamido-bridged bis(β-cyclodextrin)	15:60	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	0	44	theme	diamido-bridged	26:40	arg1	β-cyclodextrin					46:59	β-cyclodextrin	46:59	β-cyclodextrin	46:59	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	5	45	theme	mobile	1108:1113	arg1	composition					1121:1131	the mobile phase composition	1104:1131	the mobile phase composition	1104:1131	The chromatographic conditions that affect the enantioselectivity or diasterioselectivity of SBCDP were investigated in detail, including the mobile phase composition, pH value and column temperature.					
31740032	6	46	theme	tested	1184:1189	arg1	result					1172:1177	a result	1170:1177	a result	1170:1177	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	6	46	theme	tested	1184:1189	arg1	analytes					1191:1198	all tested analytes	1180:1198	all tested analytes	1180:1198	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	10	47	theme	high	2071:2074	arg1	enantioselectivity					2076:2093	high enantioselectivity	2071:2093	high enantioselectivity	2071:2093	Moreover, we also found that SBCDP possessed high enantioselectivity and diastereoselectivity over a wide range of temperature (30∼60 °C), which made the fast analysis possible.					
31740032	11	48	theme	compounds	2297:2305	arg1	analysis					2278:2285	analysis	2278:2285	analysis of chiral compounds	2278:2305	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	3	49	theme	thermogravimetric	672:688	arg1	analysis					690:697	thermogravimetric analysis	672:697	thermogravimetric analysis	672:697	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	3	50	theme	bis	523:525	arg1	structures					497:506	The structures	493:506	The structures of the bridged bis(β-cyclodextrin) and SBCDP	493:551	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	2	51	theme	ordered	381:387	arg1	SBA-15					400:405	an ordered mesoporous SBA-15	378:405	an ordered mesoporous SBA-15	378:405	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	6	52	theme	imazalil	1333:1340	arg1	resolutions					1304:1314	the enantioseparation resolutions	1282:1314	the enantioseparation resolutions of flavanone and imazalil	1282:1340	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	6	52	theme	imazalil	1333:1340	arg1	5.15					1353:1356	5.15	1353:1356	5.15	1353:1356	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	10	53	theme	fast	2180:2183	arg1	analysis					2185:2192	the fast analysis	2176:2192	the fast analysis	2176:2192	Moreover, we also found that SBCDP possessed high enantioselectivity and diastereoselectivity over a wide range of temperature (30∼60 °C), which made the fast analysis possible.					
31740032	1	54	theme	4,4'-stilbene	271:283	arg1	acid					298:301	4,4'-stilbene dicarboxylic acid	271:301	4,4'-stilbene dicarboxylic acid	271:301	A stilbene diamido-bridged bis(β-cyclodextrin) was synthesized via the reaction between 4,4'-stilbene dicarboxylic acid and 6-deoxy-6-amino-β-cyclodextrin.					
31740032	7	55	theme	chromatographic	1471:1485	arg1	performance					1487:1497	a better chromatographic performance	1462:1497	a better chromatographic performance	1462:1497	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	11	56	theme	wider	2256:2260	arg1	material					2231:2238	a new chiral separation material	2207:2238	a new chiral separation material	2207:2238	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	11	56	theme	wider	2256:2260	arg1	applications					2262:2273	wider applications	2256:2273	wider applications	2256:2273	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	6	57	theme	flavanone	1319:1327	arg1	resolutions					1304:1314	the enantioseparation resolutions	1282:1314	the enantioseparation resolutions of flavanone and imazalil	1282:1340	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	6	57	theme	flavanone	1319:1327	arg1	5.15					1353:1356	5.15	1353:1356	5.15	1353:1356	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	10	58	theme	wide	2127:2130	arg1	range					2132:2136	a wide range	2125:2136	a wide range	2125:2136	Moreover, we also found that SBCDP possessed high enantioselectivity and diastereoselectivity over a wide range of temperature (30∼60 °C), which made the fast analysis possible.					
31740032	1	59	theme	stilbene	185:192	arg1	β-cyclodextrin					214:227	β-cyclodextrin	214:227	β-cyclodextrin	214:227	A stilbene diamido-bridged bis(β-cyclodextrin) was synthesized via the reaction between 4,4'-stilbene dicarboxylic acid and 6-deoxy-6-amino-β-cyclodextrin.					
31740032	1	59	theme	stilbene	185:192	arg1	bis					210:212	A stilbene diamido-bridged bis	183:212	A stilbene diamido-bridged bis(β-cyclodextrin)	183:228	A stilbene diamido-bridged bis(β-cyclodextrin) was synthesized via the reaction between 4,4'-stilbene dicarboxylic acid and 6-deoxy-6-amino-β-cyclodextrin.					
31740032	8	60	with	SBCDP	1683:1687	arg1	resolutions					1699:1709	high resolutions	1694:1709	high resolutions	1694:1709	For example, enantiomers of trimeprazine, praziquantel, flavanone and imazalil those could not be separated by CDCSP, were separated by SBCDP with high resolutions.					
31740032	9	61	theme	CD	1756:1757	arg1	cavity					1729:1734	the small cavity	1719:1734	the small cavity (0.65 nm) of native CD	1719:1757	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	9	61	theme	CD	1756:1757	arg1	0.65 nm					1737:1743	0.65 nm	1737:1743	0.65 nm	1737:1743	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	0	62	theme	performance	148:158	arg1	chromatography					167:180	high performance liquid chromatography	143:180	high performance liquid chromatography	143:180	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	3	63	theme	mass	579:582	arg1	spectrometry					584:595	the mass spectrometry	575:595	the mass spectrometry	575:595	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	6	64	with	SBCDP	1217:1221	arg1	1.51∼5.15					1246:1254	1.51∼5.15	1246:1254	1.51∼5.15	1246:1254	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	6	64	with	SBCDP	1217:1221	arg1	resolutions					1233:1243	high resolutions	1228:1243	high resolutions (1.51∼5.15)	1228:1255	As a result, all tested analytes were resolved on SBCDP with high resolutions (1.51∼5.15) within about 25 min, and the enantioseparation resolutions of flavanone and imazalil were up to 5.15 and 4.38, respectively.					
31740032	4	65	theme	SBCDP	735:739	arg1	performance					720:730	The chromatographic performance	700:730	The chromatographic performance of SBCDP	700:739	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	5	66	theme	chromatographic	970:984	arg1	conditions					986:995	The chromatographic conditions	966:995	The chromatographic conditions that affect the enantioselectivity or diasterioselectivity of SBCDP	966:1063	The chromatographic conditions that affect the enantioselectivity or diasterioselectivity of SBCDP were investigated in detail, including the mobile phase composition, pH value and column temperature.					
31740032	4	67	theme	organic	952:958	arg1	mode					960:963	the polar organic mode	942:963	the polar organic mode	942:963	The chromatographic performance of SBCDP was systematically evaluated by separating 23 racemic drugs and pesticides, including trimeprazine, praziquantel, flavanones, β-blockers and triazole pesticides in the reversed-phase chromatography or the polar organic mode.					
31740032	7	68	theme	β-cyclodextrin	1407:1420	arg1	phase					1433:1437	the native β-cyclodextrin stationary phase	1396:1437	the native β-cyclodextrin stationary phase (CDCSP)	1396:1445	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	7	68	theme	β-cyclodextrin	1407:1420	arg1	CDCSP					1440:1444	CDCSP	1440:1444	CDCSP	1440:1444	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	9	69	theme	chiral	1981:1986	arg1	discrimination					1988:2001	the chiral discrimination	1977:2001	the chiral discrimination of SBCDP more precise	1977:2023	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	5	70	theme	pH	1134:1135	arg1	value					1137:1141	pH value	1134:1141	pH value	1134:1141	The chromatographic conditions that affect the enantioselectivity or diasterioselectivity of SBCDP were investigated in detail, including the mobile phase composition, pH value and column temperature.					
31740032	3	71	theme	magnetic	606:613	arg1	resonance					615:623	nuclear magnetic resonance	598:623	nuclear magnetic resonance	598:623	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	11	72	theme	chiral	2213:2218	arg1	material					2231:2238	a new chiral separation material	2207:2238	a new chiral separation material	2207:2238	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	11	72	theme	chiral	2213:2218	arg1	applications					2262:2273	wider applications	2256:2273	wider applications	2256:2273	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	5	73	dep	enantioselectivity	1013:1030	arg1	the					1009:1011	the	1009:1011	the	1009:1011	The chromatographic conditions that affect the enantioselectivity or diasterioselectivity of SBCDP were investigated in detail, including the mobile phase composition, pH value and column temperature.					
31740032	0	74	theme	stationary	76:85	arg1	phase					87:91	chiral stationary phase	69:91	chiral stationary phase for enantioseparations of drugs and pesticides	69:138	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	11	75	contain	have	2251:2254	arg2	material					2231:2238	a new chiral separation material	2207:2238	a new chiral separation material	2207:2238	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	11	75	contain	have	2251:2254	arg2	applications					2262:2273	wider applications	2256:2273	wider applications	2256:2273	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	11	75	contain	have	2251:2254	arg1	SBCDP					2241:2245	SBCDP	2241:2245	SBCDP	2241:2245	As a new chiral separation material, SBCDP may have wider applications in analysis of chiral compounds.					
31740032	9	76	theme	bis	1795:1797	arg1	linker					1773:1778	the bridging linker	1760:1778	the bridging linker of the bridged bis(β-CD)	1760:1803	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	8	77	theme	trimeprazine	1575:1586	arg1	enantiomers					1560:1570	enantiomers	1560:1570	enantiomers	1560:1570	For example, enantiomers of trimeprazine, praziquantel, flavanone and imazalil those could not be separated by CDCSP, were separated by SBCDP with high resolutions.					
31740032	8	78	theme	praziquantel	1589:1600	arg1	enantiomers					1560:1570	enantiomers	1560:1570	enantiomers	1560:1570	For example, enantiomers of trimeprazine, praziquantel, flavanone and imazalil those could not be separated by CDCSP, were separated by SBCDP with high resolutions.					
31740032	8	79	theme	flavanone	1603:1611	arg1	enantiomers					1560:1570	enantiomers	1560:1570	enantiomers	1560:1570	For example, enantiomers of trimeprazine, praziquantel, flavanone and imazalil those could not be separated by CDCSP, were separated by SBCDP with high resolutions.					
31740032	9	80	theme	well-organized	1816:1829	arg1	"					1845:1845	a well-organized "pseudo-cavity"	1814:1845	a well-organized "pseudo-cavity"	1814:1845	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	8	81	theme	imazalil	1617:1624	arg1	enantiomers					1560:1570	enantiomers	1560:1570	enantiomers	1560:1570	For example, enantiomers of trimeprazine, praziquantel, flavanone and imazalil those could not be separated by CDCSP, were separated by SBCDP with high resolutions.					
31740032	0	82	theme	stilbene	17:24	arg1	bis					42:44	a stilbene diamido-bridged bis	15:44	a stilbene diamido-bridged bis(β-cyclodextrin)	15:60	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	0	82	theme	stilbene	17:24	arg1	β-cyclodextrin					46:59	β-cyclodextrin	46:59	β-cyclodextrin	46:59	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	9	83	theme	pseudo-cavity	1832:1844	arg1	"					1845:1845	a well-organized "pseudo-cavity"	1814:1845	a well-organized "pseudo-cavity"	1814:1845	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	3	84	theme	elemental	649:657	arg1	analysis					659:666	elemental analysis	649:666	elemental analysis	649:666	The structures of the bridged bis(β-cyclodextrin) and SBCDP were characterized by the mass spectrometry, nuclear magnetic resonance, infrared spectroscopy, elemental analysis and thermogravimetric analysis.					
31740032	9	85	theme	bulky	1954:1958	arg1	analytes					1960:1967	some bulky analytes	1949:1967	some bulky analytes	1949:1967	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	2	86	theme	SBA-15	400:405	arg1	surface					367:373	the surface	363:373	the surface of an ordered mesoporous SBA-15	363:405	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	0	87	theme	bis	42:44	arg1	Preparation					0:10	Preparation	0:10	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)	0:60	Preparation of a stilbene diamido-bridged bis(β-cyclodextrin)-bonded chiral stationary phase for enantioseparations of drugs and pesticides by high performance liquid chromatography.					
31740032	10	88	contain	possessed	2061:2069	arg2	diastereoselectivity					2099:2118	diastereoselectivity	2099:2118	diastereoselectivity	2099:2118	Moreover, we also found that SBCDP possessed high enantioselectivity and diastereoselectivity over a wide range of temperature (30∼60 °C), which made the fast analysis possible.					
31740032	10	88	contain	possessed	2061:2069	arg1	SBCDP					2055:2059	SBCDP	2055:2059	SBCDP	2055:2059	Moreover, we also found that SBCDP possessed high enantioselectivity and diastereoselectivity over a wide range of temperature (30∼60 °C), which made the fast analysis possible.					
31740032	10	88	contain	possessed	2061:2069	arg2	enantioselectivity					2076:2093	high enantioselectivity	2071:2093	high enantioselectivity	2071:2093	Moreover, we also found that SBCDP possessed high enantioselectivity and diastereoselectivity over a wide range of temperature (30∼60 °C), which made the fast analysis possible.					
31740032	2	89	theme	bridged	425:431	arg1	SBCDP					485:489	SBCDP	485:489	SBCDP	485:489	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	2	89	theme	bridged	425:431	arg1	phase					478:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase	417:482	a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP)	417:490	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
31740032	7	90	contain	had	1458:1460	arg2	performance					1487:1497	a better chromatographic performance	1462:1497	a better chromatographic performance	1462:1497	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	7	90	contain	had	1458:1460	arg1	SBCDP					1452:1456	the SBCDP	1448:1456	the SBCDP	1448:1456	Compared with the native β-cyclodextrin stationary phase (CDCSP), the SBCDP had a better chromatographic performance in enantioselectivity and diasterioselectivity.					
31740032	9	91	theme	native	1865:1870	arg1	CDs					1872:1874	two native CDs	1861:1874	two native CDs	1861:1874	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	9	91	theme	native	1865:1870	arg1	whole					1890:1894	whole	1890:1894	whole	1890:1894	Unlike the small cavity (0.65 nm) of native CD, the bridging linker of the bridged bis(β-CD) supplied a well-organized "pseudo-cavity", and combined two native CDs as an organic whole, which could synergistically encapsulate and complex some bulky analytes, making the chiral discrimination of SBCDP more precise.					
31740032	2	92	theme	mesoporous	389:398	arg1	SBA-15					400:405	an ordered mesoporous SBA-15	378:405	an ordered mesoporous SBA-15	378:405	Then it was bonded onto the surface of an ordered mesoporous SBA-15 to obtain a novel bridged bis(β-cyclodextrin)-bonded chiral stationary phase (SBCDP).					
29509798	9	0	theme	Se-free	1648:1654	arg1	material					1662:1669	Se-free plant material	1648:1669	Se-free plant material	1648:1669	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	3	1	theme	Se	619:620	arg1	cycling					622:628	the Se cycling	615:628	the Se cycling within the Critical Zone	615:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	7	2	theme	multi-element	1430:1442	arg1	solution					1402:1409	pure Se solution	1394:1409	pure Se solution	1394:1409	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	2	theme	multi-element	1430:1442	arg1	solution					1453:1460	high concentrated multi-element standard solution	1412:1460	high concentrated multi-element standard solution as well as plant and phytoagar	1412:1491	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	10	3	theme	bias	1952:1955	arg1	correction					1957:1966	valid mass bias correction	1941:1966	valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰	1941:2083	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	6	4	theme	organic	1249:1255	arg1	residues					1257:1264	unfavorable organic residues	1237:1264	unfavorable organic residues	1237:1264	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	7	5	theme	high	1412:1415	arg1	solution					1402:1409	pure Se solution	1394:1409	pure Se solution	1394:1409	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	5	theme	high	1412:1415	arg1	solution					1453:1460	high concentrated multi-element standard solution	1412:1460	high concentrated multi-element standard solution as well as plant and phytoagar	1412:1491	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	3	6	from	metabolism	590:599	arg1	plants					604:609	plants	604:609	plants	604:609	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	3	6	from	metabolism	590:599	arg1	cycling					622:628	the Se cycling	615:628	the Se cycling within the Critical Zone	615:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	3	7	theme	Critical	641:648	arg1	Zone					650:653	the Critical Zone	637:653	the Critical Zone	637:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	4	8	theme	specific	824:831	arg1	preparation					833:843	specific preparation and purification steps	824:866	preparation	833:843	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	6	9	theme	plant	1144:1148	arg1	tissue					1150:1155	plant tissue	1144:1155	plant tissue	1144:1155	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	11	10	theme	‰	2185:2185	arg1	deviation					2126:2134	a higher deviation	2117:2134	a higher deviation of 1.1 ‰ from the true value	2117:2163	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	11	10	theme	‰	2185:2185	arg1	SD					2173:2174	a 2 SD	2169:2174	a 2 SD of ± 0.1 ‰	2169:2185	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	2	11	theme	various	274:280	arg1	patterns					290:297	various disease patterns	274:297	various disease patterns (i.e. Se excess and deficiency)	274:329	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	11	theme	various	274:280	arg1	deficiency					319:328	deficiency	319:328	deficiency	319:328	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	11	theme	various	274:280	arg1	excess					308:313	i.e. Se excess	300:313	i.e. Se excess	300:313	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	1	12	theme	tolerance	160:168	arg1	range					170:174	a narrow tolerance range	151:174	a narrow tolerance range for many organisms	151:193	Selenium (Se) is an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms.					
29509798	4	13	theme	Se	896:897	arg1	analytics					907:915	precise and valid Se isotope analytics	878:915	precise and valid Se isotope analytics performed with HG-MC-ICP-MS	878:943	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	7	14	theme	pure	1394:1397	arg1	solution					1402:1409	pure Se solution	1394:1409	pure Se solution	1394:1409	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	14	theme	pure	1394:1397	arg1	solution					1453:1460	high concentrated multi-element standard solution	1412:1460	high concentrated multi-element standard solution as well as plant and phytoagar	1412:1491	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	14	theme	pure	1394:1397	arg1	plant					1473:1477	plant	1473:1477	plant	1473:1477	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	12	15	theme	respective	2381:2390	arg1	values					2401:2406	respective δ82/76Se values	2381:2406	respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite	2381:2469	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	2	16	dep	patterns	290:297	arg1	patterns					290:297	various disease patterns	274:297	various disease patterns (i.e. Se excess and deficiency)	274:329	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	16	dep	patterns	290:297	arg1	deficiency					319:328	deficiency	319:328	deficiency	319:328	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	16	dep	patterns	290:297	arg1	excess					308:313	i.e. Se excess	300:313	i.e. Se excess	300:313	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	5	17	theme	isotope	1038:1044	arg1	measurements					1046:1057	Se isotope measurements	1035:1057	Se isotope measurements	1035:1057	In this study, different methods for the entire process from solid tissue preparation to Se isotope measurements were tested, optimized and validated.					
29509798	3	18	theme	redox	498:502	arg1	processes					504:512	redox processes	498:512	redox processes	498:512	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	9	19	theme	known	1714:1718	arg1	composition					1731:1741	known Se isotope composition	1714:1741	known Se isotope composition	1714:1741	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	6	20	theme	Se	1214:1215	arg1	recoveries					1217:1226	full Se recoveries	1209:1226	full Se recoveries	1209:1226	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	3	21	from	cycling	622:628	arg1	exploration					550:560	the exploration	546:560	the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone	546:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	9	22	theme	isotope	1723:1729	arg1	composition					1731:1741	known Se isotope composition	1714:1741	known Se isotope composition	1714:1741	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	10	23	with	samples	1978:1984	arg1	reproducibility					2051:2065	a reproducibility	2049:2065	a reproducibility (2 SD) of ± 0.2 ‰	2049:2083	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	23	with	samples	1978:1984	arg1	SD					2070:2071	2 SD	2068:2071	2 SD	2068:2071	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	23	with	samples	1978:1984	arg1	deviation					2002:2010	an average deviation	1991:2010	an average deviation to true δ82/76Se values of 0.2 ‰	1991:2043	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	24	theme	detectable	1868:1877	arg1	trapping					1920:1927	trapping	1920:1927	trapping	1920:1927	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	24	theme	detectable	1868:1877	arg1	residues					1887:1894	no detectable organic residues	1865:1894	no detectable organic residues	1865:1894	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	24	theme	detectable	1868:1877	arg1	generation					1905:1914	hydride generation	1897:1914	hydride generation	1897:1914	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	5	25	theme	tissue	1013:1018	arg1	preparation					1020:1030	solid tissue preparation	1007:1030	solid tissue preparation	1007:1030	In this study, different methods for the entire process from solid tissue preparation to Se isotope measurements were tested, optimized and validated.					
29509798	1	26	theme	important	98:106	arg1	micronutrient					108:120	an important micronutrient	95:120	an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms	95:193	Selenium (Se) is an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms.					
29509798	1	26	theme	important	98:106	arg1	Selenium					78:85	Selenium	78:85	Selenium (Se)	78:90	Selenium (Se) is an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms.					
29509798	12	27	theme	methods	2221:2227	arg1	application					2192:2202	The application	2188:2202	The application of the developed methods to cultivated plants	2188:2248	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	4	28	theme	Plant	656:660	arg1	setups					674:679	Plant cultivation setups	656:679	Plant cultivation setups	656:679	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	6	29	theme	vacuum	1163:1168	arg1	method					1181:1186	a vacuum filtration method	1161:1186	a vacuum filtration method for phytoagar	1161:1200	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	12	30	dep	‰	2424:2424	arg1	to					2416:2417	to	2416:2417	to	2416:2417	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	9	31	theme	high	1756:1759	arg1	impact					1761:1766	the high impact	1752:1766	the high impact of organic residues on the accuracy of MC-ICP-MS measurements	1752:1828	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	12	32	theme	cultivated	2232:2241	arg1	plants					2243:2248	cultivated plants	2232:2248	cultivated plants	2232:2248	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	10	33	theme	true	2015:2018	arg1	values					2029:2034	true δ82/76Se values	2015:2034	true δ82/76Se values of 0.2 ‰	2015:2043	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	0	34	theme	isotope	61:67	arg1	studies					69:75	selenium isotope studies	52:75	selenium isotope studies	52:75	Preparation and purification of organic samples for selenium isotope studies.					
29509798	11	35	theme	higher	2119:2124	arg1	deviation					2126:2134	a higher deviation	2117:2134	a higher deviation of 1.1 ‰ from the true value	2117:2163	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	11	36	theme	true	2154:2157	arg1	value					2159:2163	the true value	2150:2163	the true value	2150:2163	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	5	37	from	preparation	1020:1030	arg1	process					994:1000	the entire process	983:1000	the entire process from solid tissue preparation to Se isotope measurements	983:1057	In this study, different methods for the entire process from solid tissue preparation to Se isotope measurements were tested, optimized and validated.					
29509798	1	38	with	toxin	140:144	arg1	range					170:174	a narrow tolerance range	151:174	a narrow tolerance range for many organisms	151:193	Selenium (Se) is an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms.					
29509798	10	39	theme	organic	1879:1885	arg1	trapping					1920:1927	trapping	1920:1927	trapping	1920:1927	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	39	theme	organic	1879:1885	arg1	residues					1887:1894	no detectable organic residues	1865:1894	no detectable organic residues	1865:1894	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	39	theme	organic	1879:1885	arg1	generation					1905:1914	hydride generation	1897:1914	hydride generation	1897:1914	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	9	40	theme	residues	1779:1786	arg1	impact					1761:1766	the high impact	1752:1766	the high impact of organic residues on the accuracy of MC-ICP-MS measurements	1752:1828	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	2	41	theme	heterogeneous	216:228	arg1	distribution					233:244	a globally heterogeneous Se distribution	205:244	a globally heterogeneous Se distribution in soils	205:253	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	41	theme	heterogeneous	216:228	arg1	responsible					258:268	responsible	258:268	responsible	258:268	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	3	42	from	exploration	550:560	arg1	plants					604:609	plants	604:609	plants	604:609	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	3	42	from	exploration	550:560	arg1	cycling					622:628	the Se cycling	615:628	the Se cycling within the Critical Zone	615:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	10	43	theme	mass	1947:1950	arg1	correction					1957:1966	valid mass bias correction	1941:1966	valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰	1941:2083	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	2	44	theme	environmental	335:347	arg1	problems					349:356	environmental problems	335:356	environmental problems	335:356	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	12	45	dep	+1.9	2451:2454	arg1	to					2448:2449	to	2448:2449	to	2448:2449	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	7	46	theme	purification	1299:1310	arg1	methods					1312:1318	Three purification methods	1293:1318	Three purification methods predominantly described in the literature	1293:1360	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	46	theme	purification	1299:1310	arg1	matrices					1503:1510	target matrices	1496:1510	target matrices	1496:1510	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	9	47	theme	measurements	1817:1828	arg1	accuracy					1795:1802	the accuracy	1791:1802	the accuracy of MC-ICP-MS measurements	1791:1828	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	8	48	theme	critical	1550:1557	arg1	elements					1566:1573	critical matrix elements	1550:1573	critical matrix elements	1550:1573	All these methods efficiently remove critical matrix elements, but differ in Se recovery and organic residues.					
29509798	4	49	theme	systematic	688:697	arg1	investigations					710:723	systematic controlled investigations	688:723	systematic controlled investigations	688:723	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	3	50	theme	metabolism	590:599	arg1	exploration					550:560	the exploration	546:560	the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone	546:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	0	51	theme	organic	32:38	arg1	samples					40:46	organic samples	32:46	organic samples	32:46	Preparation and purification of organic samples for selenium isotope studies.					
29509798	12	52	theme	plant	2329:2333	arg1	fractionations					2355:2368	plant internal Se isotope fractionations	2329:2368	plant internal Se isotope fractionations	2329:2368	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	3	53	theme	dependent	577:585	arg1	metabolism					590:599	the species dependent Se metabolism	565:599	the species dependent Se metabolism in plants and the Se cycling within the Critical Zone	565:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	10	54	theme	valid	1941:1945	arg1	correction					1957:1966	valid mass bias correction	1941:1966	valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰	1941:2083	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	3	55	theme	Selenium	430:437	arg1	tracer					487:492	a powerful tracer	476:492	a powerful tracer for redox processes	476:512	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	3	55	theme	Selenium	430:437	arg1	variations					447:456	Selenium isotope variations	430:456	Selenium isotope variations	430:456	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	5	56	theme	solid	1007:1011	arg1	preparation					1020:1030	solid tissue preparation	1007:1030	solid tissue preparation	1007:1030	In this study, different methods for the entire process from solid tissue preparation to Se isotope measurements were tested, optimized and validated.					
29509798	6	57	theme	particular	1099:1108	arg1	procedure					1130:1138	A particular microwave digestion procedure	1097:1138	A particular microwave digestion procedure for plant tissue	1097:1155	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	12	58	theme	Se	2344:2345	arg1	fractionations					2355:2368	plant internal Se isotope fractionations	2329:2368	plant internal Se isotope fractionations	2329:2368	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	10	59	theme	hydride	1897:1903	arg1	residues					1887:1894	no detectable organic residues	1865:1894	no detectable organic residues	1865:1894	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	59	theme	hydride	1897:1903	arg1	generation					1905:1914	hydride generation	1897:1914	hydride generation	1897:1914	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	9	60	with	phytoagar	1675:1683	arg1	material					1702:1709	a reference material	1690:1709	a reference material	1690:1709	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	7	61	theme	target	1496:1501	arg1	methods					1312:1318	Three purification methods	1293:1318	Three purification methods predominantly described in the literature	1293:1360	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	61	theme	target	1496:1501	arg1	matrices					1503:1510	target matrices	1496:1510	target matrices	1496:1510	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	9	62	theme	Validation	1624:1633	arg1	tests					1635:1639	Validation tests	1624:1639	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition	1624:1741	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	12	63	theme	‰	2424:2424	arg1	values					2401:2406	respective δ82/76Se values	2381:2406	respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite	2381:2469	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	4	64	theme	them-plant	751:760	arg1	tissue					762:767	them-plant tissue	751:767	them-plant tissue	751:767	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	9	65	with	material	1662:1669	arg1	material					1702:1709	a reference material	1690:1709	a reference material	1690:1709	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	10	66	theme	±	2077:2077	arg1	‰					2083:2083	± 0.2 ‰	2077:2083	± 0.2 ‰	2077:2083	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	8	67	theme	organic	1606:1612	arg1	residues					1614:1621	organic residues	1606:1621	organic residues	1606:1621	All these methods efficiently remove critical matrix elements, but differ in Se recovery and organic residues.					
29509798	9	68	theme	plant	1656:1660	arg1	material					1662:1669	Se-free plant material	1648:1669	Se-free plant material	1648:1669	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	3	69	theme	powerful	478:485	arg1	tracer					487:492	a powerful tracer	476:492	a powerful tracer for redox processes	476:512	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	3	69	theme	powerful	478:485	arg1	variations					447:456	Selenium isotope variations	430:456	Selenium isotope variations	430:456	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	4	70	theme	purification	849:860	arg1	steps					862:866	specific preparation and purification steps	824:866	steps	862:866	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	7	71	theme	standard	1444:1451	arg1	solution					1402:1409	pure Se solution	1394:1409	pure Se solution	1394:1409	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	71	theme	standard	1444:1451	arg1	solution					1453:1460	high concentrated multi-element standard solution	1412:1460	high concentrated multi-element standard solution as well as plant and phytoagar	1412:1491	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	12	72	theme	+3.5	2419:2422	arg1	‰					2424:2424	+2.3 to +3.5 ‰	2411:2424	+2.3 to +3.5 ‰	2411:2424	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	2	73	theme	Se	305:306	arg1	patterns					290:297	various disease patterns	274:297	various disease patterns (i.e. Se excess and deficiency)	274:329	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	73	theme	Se	305:306	arg1	excess					308:313	i.e. Se excess	300:313	i.e. Se excess	300:313	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	6	74	theme	unfavorable	1237:1247	arg1	residues					1257:1264	unfavorable organic residues	1237:1264	unfavorable organic residues	1237:1264	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	2	75	theme	Se	398:399	arg1	entrance					401:408	the Se entrance	394:408	the Se entrance into the biosphere	394:427	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	7	76	theme	concentrated	1417:1428	arg1	solution					1402:1409	pure Se solution	1394:1409	pure Se solution	1394:1409	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	76	theme	concentrated	1417:1428	arg1	solution					1453:1460	high concentrated multi-element standard solution	1412:1460	high concentrated multi-element standard solution as well as plant and phytoagar	1412:1491	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	10	77	theme	‰	2083:2083	arg1	reproducibility					2051:2065	a reproducibility	2049:2065	a reproducibility (2 SD) of ± 0.2 ‰	2049:2083	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	77	theme	‰	2083:2083	arg1	SD					2070:2071	2 SD	2068:2071	2 SD	2068:2071	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	77	theme	‰	2083:2083	arg1	deviation					2002:2010	an average deviation	1991:2010	an average deviation to true δ82/76Se values of 0.2 ‰	1991:2043	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	12	78	theme	isotope	2347:2353	arg1	fractionations					2355:2368	plant internal Se isotope fractionations	2329:2368	plant internal Se isotope fractionations	2329:2368	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	2	79	theme	disease	282:288	arg1	patterns					290:297	various disease patterns	274:297	various disease patterns (i.e. Se excess and deficiency)	274:329	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	79	theme	disease	282:288	arg1	deficiency					319:328	deficiency	319:328	deficiency	319:328	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	79	theme	disease	282:288	arg1	excess					308:313	i.e. Se excess	300:313	i.e. Se excess	300:313	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	1	80	theme	narrow	153:158	arg1	range					170:174	a narrow tolerance range	151:174	a narrow tolerance range for many organisms	151:193	Selenium (Se) is an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms.					
29509798	9	81	theme	reference	1692:1700	arg1	material					1702:1709	a reference material	1690:1709	a reference material	1690:1709	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	12	82	theme	δ82/76Se	2392:2399	arg1	values					2401:2406	respective δ82/76Se values	2381:2406	respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite	2381:2469	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	10	83	theme	plant	1972:1976	arg1	samples					1978:1984	plant samples	1972:1984	plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰	1972:2083	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	4	84	theme	valid	890:894	arg1	analytics					907:915	precise and valid Se isotope analytics	878:915	precise and valid Se isotope analytics performed with HG-MC-ICP-MS	878:943	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	12	85	theme	sufficient	2256:2265	arg1	accuracy					2267:2274	sufficient accuracy	2256:2274	sufficient accuracy	2256:2274	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	7	86	theme	Se	1399:1400	arg1	solution					1402:1409	pure Se solution	1394:1409	pure Se solution	1394:1409	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	86	theme	Se	1399:1400	arg1	solution					1453:1460	high concentrated multi-element standard solution	1412:1460	high concentrated multi-element standard solution as well as plant and phytoagar	1412:1491	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	7	86	theme	Se	1399:1400	arg1	plant					1473:1477	plant	1473:1477	plant	1473:1477	Three purification methods predominantly described in the literature were systematically tested with pure Se solution, high concentrated multi-element standard solution as well as plant and phytoagar as target matrices.					
29509798	3	87	theme	isotope	439:445	arg1	tracer					487:492	a powerful tracer	476:492	a powerful tracer for redox processes	476:512	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	3	87	theme	isotope	439:445	arg1	variations					447:456	Selenium isotope variations	430:456	Selenium isotope variations	430:456	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	1	88	theme	many	180:183	arg1	organisms					185:193	many organisms	180:193	many organisms	180:193	Selenium (Se) is an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms.					
29509798	11	89	theme	‰	2143:2143	arg1	deviation					2126:2134	a higher deviation	2117:2134	a higher deviation of 1.1 ‰ from the true value	2117:2163	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	11	89	theme	‰	2143:2143	arg1	SD					2173:2174	a 2 SD	2169:2174	a 2 SD of ± 0.1 ‰	2169:2185	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	9	90	theme	Se	1720:1721	arg1	composition					1731:1741	known Se isotope composition	1714:1741	known Se isotope composition	1714:1741	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	6	91	theme	full	1209:1212	arg1	recoveries					1217:1226	full Se recoveries	1209:1226	full Se recoveries	1209:1226	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	10	92	theme	average	1994:2000	arg1	deviation					2002:2010	an average deviation	1991:2010	an average deviation to true δ82/76Se values of 0.2 ‰	1991:2043	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	93	with	method	1853:1858	arg1	trapping					1920:1927	trapping	1920:1927	trapping	1920:1927	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	93	with	method	1853:1858	arg1	residues					1887:1894	no detectable organic residues	1865:1894	no detectable organic residues	1865:1894	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	93	with	method	1853:1858	arg1	generation					1905:1914	hydride generation	1897:1914	hydride generation	1897:1914	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	9	94	theme	composition	1731:1741	arg1	material					1662:1669	Se-free plant material	1648:1669	Se-free plant material	1648:1669	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	9	94	theme	composition	1731:1741	arg1	phytoagar					1675:1683	phytoagar	1675:1683	phytoagar with a reference material of known Se isotope composition	1675:1741	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	11	95	from	value	2159:2163	arg1	deviation					2126:2134	a higher deviation	2117:2134	a higher deviation of 1.1 ‰ from the true value	2117:2163	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	11	95	from	value	2159:2163	arg1	‰					2143:2143	1.1 ‰	2139:2143	1.1 ‰ from the true value	2139:2163	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	11	95	from	value	2159:2163	arg1	SD					2173:2174	a 2 SD	2169:2174	a 2 SD of ± 0.1 ‰	2169:2185	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	2	96	theme	Se	230:231	arg1	distribution					233:244	a globally heterogeneous Se distribution	205:244	a globally heterogeneous Se distribution in soils	205:253	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	96	theme	Se	230:231	arg1	responsible					258:268	responsible	258:268	responsible	258:268	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	97	from	distribution	233:244	arg1	soils					249:253	soils	249:253	soils	249:253	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	4	98	theme	precise	878:884	arg1	analytics					907:915	precise and valid Se isotope analytics	878:915	precise and valid Se isotope analytics performed with HG-MC-ICP-MS	878:943	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	10	99	theme	purification	1840:1851	arg1	method					1853:1858	Only the purification method	1831:1858	Only the purification method with no detectable organic residues, hydride generation and trapping,	1831:1928	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	10	100	theme	δ82/76Se	2020:2027	arg1	values					2029:2034	true δ82/76Se values	2015:2034	true δ82/76Se values of 0.2 ‰	2015:2043	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	5	101	theme	Se	1035:1036	arg1	measurements					1046:1057	Se isotope measurements	1035:1057	Se isotope measurements	1035:1057	In this study, different methods for the entire process from solid tissue preparation to Se isotope measurements were tested, optimized and validated.					
29509798	6	102	theme	filtration	1170:1179	arg1	method					1181:1186	a vacuum filtration method	1161:1186	a vacuum filtration method for phytoagar	1161:1200	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	1	103	theme	strong	133:138	arg1	toxin					140:144	a strong toxin	131:144	an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms	95:193	Selenium (Se) is an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms.					
29509798	9	104	theme	organic	1771:1777	arg1	residues					1779:1786	organic residues	1771:1786	organic residues	1771:1786	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	12	105	theme	developed	2211:2219	arg1	methods					2221:2227	the developed methods	2207:2227	the developed methods	2207:2227	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	4	106	attach	derived	738:744	arg2	samples					730:736	samples	730:736	samples derived from them-plant tissue and phytoagar-are	730:785	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	4	106	attach	derived	738:744	arg1	phytoagar-are					773:785	phytoagar-are	773:785	phytoagar-are	773:785	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	4	106	attach	derived	738:744	arg1	tissue					762:767	them-plant tissue	751:767	them-plant tissue	751:767	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	6	107	theme	digestion	1120:1128	arg1	procedure					1130:1138	A particular microwave digestion procedure	1097:1138	A particular microwave digestion procedure for plant tissue	1097:1155	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	11	108	theme	±	2179:2179	arg1	‰					2185:2185	± 0.1 ‰	2179:2185	± 0.1 ‰	2179:2185	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	4	109	theme	controlled	699:708	arg1	investigations					710:723	systematic controlled investigations	688:723	systematic controlled investigations	688:723	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	1	110	with	micronutrient	108:120	arg1	range					170:174	a narrow tolerance range	151:174	a narrow tolerance range for many organisms	151:193	Selenium (Se) is an important micronutrient but also a strong toxin with a narrow tolerance range for many organisms.					
29509798	12	111	theme	promising	2299:2307	arg1	approach					2309:2316	a promising approach	2297:2316	a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained	2297:2483	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	9	112	theme	MC-ICP-MS	1807:1815	arg1	measurements					1817:1828	MC-ICP-MS measurements	1807:1828	MC-ICP-MS measurements	1807:1828	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	8	113	theme	matrix	1559:1564	arg1	elements					1566:1573	critical matrix elements	1550:1573	critical matrix elements	1550:1573	All these methods efficiently remove critical matrix elements, but differ in Se recovery and organic residues.					
29509798	4	114	theme	cultivation	662:672	arg1	setups					674:679	Plant cultivation setups	656:679	Plant cultivation setups	656:679	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	10	115	theme	‰	2043:2043	arg1	values					2029:2034	true δ82/76Se values	2015:2034	true δ82/76Se values of 0.2 ‰	2015:2043	Only the purification method with no detectable organic residues, hydride generation and trapping, results in valid mass bias correction for plant samples with an average deviation to true δ82/76Se values of 0.2 ‰ and a reproducibility (2 SD) of ± 0.2 ‰.					
29509798	2	116	theme	key	381:383	arg1	role					385:388	a key role	379:388	a key role	379:388	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	3	117	theme	Se	587:588	arg1	metabolism					590:599	the species dependent Se metabolism	565:599	the species dependent Se metabolism in plants and the Se cycling within the Critical Zone	565:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	0	118	theme	samples	40:46	arg1	purification					16:27	purification	16:27	purification	16:27	Preparation and purification of organic samples for selenium isotope studies.					
29509798	0	118	theme	samples	40:46	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and purification of organic samples for selenium isotope studies.					
29509798	5	119	theme	entire	987:992	arg1	process					994:1000	the entire process	983:1000	the entire process from solid tissue preparation to Se isotope measurements	983:1057	In this study, different methods for the entire process from solid tissue preparation to Se isotope measurements were tested, optimized and validated.					
29509798	12	120	theme	internal	2335:2342	arg1	fractionations					2355:2368	plant internal Se isotope fractionations	2329:2368	plant internal Se isotope fractionations	2329:2368	The application of the developed methods to cultivated plants shows sufficient accuracy and precision and is a promising approach to resolve plant internal Se isotope fractionations, for which respective δ82/76Se values of +2.3 to +3.5 ‰ for selenate and +1.2 to +1.9 ‰ for selenite were obtained.					
29509798	11	121	from	deviation	2126:2134	arg1	value					2159:2163	the true value	2150:2163	the true value	2150:2163	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
29509798	3	122	theme	species	569:575	arg1	metabolism					590:599	the species dependent Se metabolism	565:599	the species dependent Se metabolism in plants and the Se cycling within the Critical Zone	565:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	0	123	theme	selenium	52:59	arg1	studies					69:75	selenium isotope studies	52:75	selenium isotope studies	52:75	Preparation and purification of organic samples for selenium isotope studies.					
29509798	6	124	theme	microwave	1110:1118	arg1	procedure					1130:1138	A particular microwave digestion procedure	1097:1138	A particular microwave digestion procedure for plant tissue	1097:1155	A particular microwave digestion procedure for plant tissue and a vacuum filtration method for phytoagar led to full Se recoveries, whereby unfavorable organic residues were reduced to a minimum.					
29509798	4	125	theme	isotope	899:905	arg1	analytics					907:915	precise and valid Se isotope analytics	878:915	precise and valid Se isotope analytics performed with HG-MC-ICP-MS	878:943	Plant cultivation setups enable systematic controlled investigations, but samples derived from them-plant tissue and phytoagar-are particularly challenging and require specific preparation and purification steps to ensure precise and valid Se isotope analytics performed with HG-MC-ICP-MS.					
29509798	8	126	theme	Se	1590:1591	arg1	recovery					1593:1600	Se recovery	1590:1600	Se recovery	1590:1600	All these methods efficiently remove critical matrix elements, but differ in Se recovery and organic residues.					
29509798	2	127	theme	i.e.	300:303	arg1	patterns					290:297	various disease patterns	274:297	various disease patterns (i.e. Se excess and deficiency)	274:329	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	2	127	theme	i.e.	300:303	arg1	excess					308:313	i.e. Se excess	300:313	i.e. Se excess	300:313	As such, a globally heterogeneous Se distribution in soils is responsible for various disease patterns (i.e. Se excess and deficiency) and environmental problems, whereby plants play a key role for the Se entrance into the biosphere.					
29509798	5	128	theme	different	961:969	arg1	methods					971:977	different methods	961:977	different methods for the entire process from solid tissue preparation to Se isotope measurements	961:1057	In this study, different methods for the entire process from solid tissue preparation to Se isotope measurements were tested, optimized and validated.					
29509798	9	129	from	impact	1761:1766	arg1	accuracy					1795:1802	the accuracy	1791:1802	the accuracy of MC-ICP-MS measurements	1791:1828	Validation tests doping Se-free plant material and phytoagar with a reference material of known Se isotope composition revealed the high impact of organic residues on the accuracy of MC-ICP-MS measurements.					
29509798	3	130	from	plants	604:609	arg1	exploration					550:560	the exploration	546:560	the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone	546:653	Selenium isotope variations were proved to be a powerful tracer for redox processes and are therefore promising for the exploration of the species dependent Se metabolism in plants and the Se cycling within the Critical Zone.					
29509798	11	131	from	SD	2173:2174	arg1	value					2159:2163	the true value	2150:2163	the true value	2150:2163	For phytoagar this test yields a higher deviation of 1.1 ‰ from the true value and a 2 SD of ± 0.1 ‰.					
31338548	7	0	theme	insulin	1449:1455	arg1	clearance					1457:1465	insulin clearance	1449:1465	insulin clearance	1449:1465	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	2	1	theme	glucose	594:600	arg1	metabolism					602:611	glucose metabolism	594:611	glucose metabolism	594:611	We hypothesized that these discrepancies are accounted for by the surrogate tools used to evaluate insulin resistance and that PET 18FDG, given its capacity to quantify insulin sensitivity in individual organs, would be more sensitive in identifying changes in glucose metabolism.					
31338548	3	2	from	effects	674:680	arg1	sensitivity					749:759	insulin sensitivity	741:759	insulin sensitivity	741:759	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	3	2	from	effects	674:680	arg1	uptake					730:735	whole-body and organ-specific glucose uptake	692:735	whole-body and organ-specific glucose uptake	692:735	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	4	3	theme	obesogenic	861:870	arg1	diet					872:875	an obesogenic diet	858:875	an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group)	858:996	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	3	4	theme	glucose	722:728	arg1	uptake					730:735	whole-body and organ-specific glucose uptake	692:735	whole-body and organ-specific glucose uptake	692:735	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	6	5	theme	Body	1229:1232	arg1	composition					1234:1244	Body composition	1229:1244	Body composition	1229:1244	Body composition was also measured using CT imaging and energy metabolism quantified with indirect calorimetry.					
31338548	1	6	theme	inconsistent	252:263	arg1	observations					265:276	the inconsistent observations	248:276	the inconsistent observations relating to insulin resistance in recent human trials	248:330	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	7	7	theme	abdominal	1385:1393	arg1	fat					1395:1397	subcutaneous abdominal fat	1372:1397	subcutaneous abdominal fat by ≈ 20%	1372:1406	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	11	8	theme	glucose	2141:2147	arg1	uptake					2149:2154	glucose uptake	2141:2154	glucose uptake	2141:2154	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	10	9	from	active	2045:2050	arg1	CONCLUSIONS					1952:1962	CONCLUSIONS	1952:1962	CONCLUSIONS	1952:1962	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	11	10	from	effects	2191:2197	arg1	brain					2243:2247	the brain	2239:2247	the brain	2239:2247	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	11	10	from	effects	2191:2197	arg1	tissue					2225:2230	the adipose tissue	2213:2230	the adipose tissue	2213:2230	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	3	11	from	uptake	730:735	arg1	evaluation					656:665	a comprehensive evaluation	640:665	a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity	640:759	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	8	12	theme	control	1654:1660	arg1	group					1667:1671	the control diet group	1650:1671	the control diet group	1650:1671	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	4	13	theme	K	933:933	arg1	0.5 mg/kg/day					935:947	sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day	896:947	0.5 mg/kg/day	935:947	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	13	theme	K	933:933	arg1	LCS					891:893	LCS	891:893	LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group)	891:964	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	7	14	theme	LCS	1353:1355	arg1	diet					1357:1360	The LCS diet	1349:1360	The LCS diet	1349:1360	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	9	15	theme	glucose	1795:1801	arg1	uptake					1803:1808	glucose uptake	1795:1808	glucose uptake	1795:1808	In the brain, glucose uptake increased significantly only in the frontal cortex, associated with improved metabolic connectivity towards the hippocampus and the amygdala.					
31338548	3	16	theme	animal	772:777	arg1	model					779:783	a large animal model	764:783	a large animal model of morbid obesity	764:801	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	5	17	theme	18FDG	1153:1157	arg1	scanning					1159:1166	dynamic PET 18FDG scanning	1141:1166	dynamic PET 18FDG scanning	1141:1166	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	1	18	theme	glucose	196:202	arg1	metabolism					204:213	glucose metabolism	196:213	glucose metabolism	196:213	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	6	19	theme	CT	1270:1271	arg1	imaging					1273:1279	CT imaging	1270:1279	CT imaging	1270:1279	Body composition was also measured using CT imaging and energy metabolism quantified with indirect calorimetry.					
31338548	4	20	theme	sucralose	896:904	arg1	group					959:963	LCS diet group	950:963	LCS diet group	950:963	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	20	theme	sucralose	896:904	arg1	1 mg/kg/day					906:916	sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day	896:947	1 mg/kg/day	906:916	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	20	theme	sucralose	896:904	arg1	LCS					891:893	LCS	891:893	LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group)	891:964	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	10	21	theme	K	2027:2027	arg1	active					2045:2050	active	2045:2050	active	2045:2050	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	10	21	theme	K	2027:2027	arg1	combination					1987:1997	the combination	1983:1997	the combination of sucralose and acesulfame K	1983:2027	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	3	22	theme	obesity	795:801	arg1	model					779:783	a large animal model	764:783	a large animal model of morbid obesity	764:801	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	8	23	from	uptake	1570:1575	arg1	brain					1604:1608	brain	1604:1608	brain	1604:1608	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	8	23	from	uptake	1570:1575	arg1	liver					1594:1598	liver	1594:1598	liver	1594:1598	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	8	23	from	uptake	1570:1575	arg1	duodenum					1584:1591	the duodenum	1580:1591	the duodenum	1580:1591	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	5	24	theme	Glucose	1013:1019	arg1	uptake					1021:1026	Glucose uptake	1013:1026	Glucose uptake	1013:1026	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	1	25	dep	PURPOSES	103:110	arg1	alter					190:194	alter	190:194	can alter glucose metabolism	186:213	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	0	26	theme	large	72:76	arg1	model					85:89	a large animal model	70:89	a large animal model of obesity	70:100	Low-calorie sweeteners augment tissue-specific insulin sensitivity in a large animal model of obesity.					
31338548	11	27	theme	whole-body	2073:2082	arg1	resistance					2092:2101	whole-body insulin resistance	2073:2101	whole-body insulin resistance	2073:2101	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	5	28	theme	input	1213:1217	arg1	function					1219:1226	arterial input function	1204:1226	arterial input function	1204:1226	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	8	29	theme	insulin	1699:1705	arg1	sensitivity					1707:1717	insulin sensitivity	1699:1717	insulin sensitivity	1699:1717	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	7	30	theme	insulin	1513:1519	arg1	sensitivity					1521:1531	insulin sensitivity	1513:1531	insulin sensitivity	1513:1531	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	11	31	theme	insulin	2117:2123	arg1	sensitivity					2125:2135	insulin sensitivity	2117:2135	insulin sensitivity	2117:2135	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	0	32	theme	obesity	94:100	arg1	model					85:89	a large animal model	70:89	a large animal model of obesity	70:100	Low-calorie sweeteners augment tissue-specific insulin sensitivity in a large animal model of obesity.					
31338548	2	33	theme	insulin	502:508	arg1	sensitivity					510:520	insulin sensitivity	502:520	insulin sensitivity	502:520	We hypothesized that these discrepancies are accounted for by the surrogate tools used to evaluate insulin resistance and that PET 18FDG, given its capacity to quantify insulin sensitivity in individual organs, would be more sensitive in identifying changes in glucose metabolism.					
31338548	9	34	theme	improved	1878:1885	arg1	connectivity					1897:1908	improved metabolic connectivity	1878:1908	improved metabolic connectivity towards the hippocampus and the amygdala	1878:1949	In the brain, glucose uptake increased significantly only in the frontal cortex, associated with improved metabolic connectivity towards the hippocampus and the amygdala.					
31338548	8	35	theme	53	1732:1733	arg1	%					1745:1745	53, 55 and 28%	1732:1745	%	1745:1745	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	1	36	theme	insulin	290:296	arg1	resistance					298:307	insulin resistance	290:307	insulin resistance in recent human trials	290:330	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	3	37	from	sensitivity	749:759	arg1	evaluation					656:665	a comprehensive evaluation	640:665	a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity	640:759	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	10	38	from	CONCLUSIONS	1952:1962	arg1	active					2045:2050	active	2045:2050	active	2045:2050	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	10	38	from	CONCLUSIONS	1952:1962	arg1	combination					1987:1997	the combination	1983:1997	the combination of sucralose and acesulfame K	1983:2027	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	0	39	theme	Low-calorie	0:10	arg1	sweeteners					12:21	Low-calorie sweeteners	0:21	Low-calorie sweeteners	0:21	Low-calorie sweeteners augment tissue-specific insulin sensitivity in a large animal model of obesity.					
31338548	2	40	theme	insulin	432:438	arg1	resistance					440:449	insulin resistance	432:449	insulin resistance	432:449	We hypothesized that these discrepancies are accounted for by the surrogate tools used to evaluate insulin resistance and that PET 18FDG, given its capacity to quantify insulin sensitivity in individual organs, would be more sensitive in identifying changes in glucose metabolism.					
31338548	3	41	theme	comprehensive	642:654	arg1	evaluation					656:665	a comprehensive evaluation	640:665	a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity	640:759	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	0	42	theme	insulin	47:53	arg1	sensitivity					55:65	tissue-specific insulin sensitivity	31:65	tissue-specific insulin sensitivity	31:65	Low-calorie sweeteners augment tissue-specific insulin sensitivity in a large animal model of obesity.					
31338548	4	43	theme	control	983:989	arg1	LCS					978:980	LCS	978:980	LCS (control group)	978:996	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	43	theme	control	983:989	arg1	group					991:995	control group	983:995	control group	983:995	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	11	44	from	sensitivity	2125:2135	arg1	tissues					2168:2174	specific tissues	2159:2174	specific tissues	2159:2174	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	1	45	dep	sucralose	158:166	arg1	K					183:183	K	183:183	K	183:183	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	5	46	theme	adipose	1110:1116	arg1	tissue					1118:1123	adipose tissue	1110:1123	adipose tissue	1110:1123	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	5	46	theme	adipose	1110:1116	arg1	duodenum					1076:1083	the duodenum	1072:1083	the duodenum	1072:1083	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	3	47	theme	effects	674:680	arg1	evaluation					656:665	a comprehensive evaluation	640:665	a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity	640:759	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	3	48	from	evaluation	656:665	arg1	sensitivity					749:759	insulin sensitivity	741:759	insulin sensitivity	741:759	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	3	48	from	evaluation	656:665	arg1	uptake					730:735	whole-body and organ-specific glucose uptake	692:735	whole-body and organ-specific glucose uptake	692:735	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	2	49	theme	PET	460:462	arg1	18FDG					464:468	PET 18FDG	460:468	PET 18FDG	460:468	We hypothesized that these discrepancies are accounted for by the surrogate tools used to evaluate insulin resistance and that PET 18FDG, given its capacity to quantify insulin sensitivity in individual organs, would be more sensitive in identifying changes in glucose metabolism.					
31338548	10	50	theme	miniature	1967:1975	arg1	pigs					1977:1980	miniature pigs	1967:1980	miniature pigs	1967:1980	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	3	51	theme	whole-body	692:701	arg1	uptake					730:735	whole-body and organ-specific glucose uptake	692:735	whole-body and organ-specific glucose uptake	692:735	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	8	52	theme	28	1743:1744	arg1	%					1745:1745	53, 55 and 28%	1732:1745	%	1745:1745	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	5	53	theme	PET	1149:1151	arg1	scanning					1159:1166	dynamic PET 18FDG scanning	1141:1166	dynamic PET 18FDG scanning	1141:1166	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	10	54	dep	CONCLUSIONS	1952:1962	arg1	pigs					1977:1980	miniature pigs	1967:1980	miniature pigs	1967:1980	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	3	55	theme	LCS	685:687	arg1	effects					674:680	the effects	670:680	the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity	670:759	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	1	56	from	resistance	298:307	arg1	trials					325:330	recent human trials	312:330	recent human trials	312:330	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	8	57	theme	57	1623:1624	arg1	%					1636:1636	57, 66 and 29%	1623:1636	%	1636:1636	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	7	58	theme	weight	1424:1429	arg1	gain					1431:1434	weight gain	1424:1434	weight gain	1424:1434	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	9	59	dep	increased	1810:1818	arg1	associated					1862:1871	associated	1862:1871	associated with improved metabolic connectivity towards the hippocampus and the amygdala	1862:1949	In the brain, glucose uptake increased significantly only in the frontal cortex, associated with improved metabolic connectivity towards the hippocampus and the amygdala.					
31338548	7	60	dep	RESULTS	1341:1347	arg1	increased					1362:1370	increased	1362:1370	increased subcutaneous abdominal fat by ≈ 20% without causing weight gain	1362:1434	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	7	60	dep	RESULTS	1341:1347	arg1	reduced					1441:1447	reduced	1441:1447	reduced insulin clearance by ≈ 40%	1441:1474	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	4	61	theme	morbid	834:839	arg1	obesity					841:847	morbid obesity	834:847	morbid obesity	834:847	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	1	62	theme	recent	312:317	arg1	trials					325:330	recent human trials	312:330	recent human trials	312:330	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	3	63	theme	organ-specific	707:720	arg1	uptake					730:735	whole-body and organ-specific glucose uptake	692:735	whole-body and organ-specific glucose uptake	692:735	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	8	64	theme	29	1634:1635	arg1	%					1636:1636	57, 66 and 29%	1623:1636	%	1636:1636	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	10	65	theme	acesulfame	2016:2025	arg1	K					2027:2027	acesulfame K	2016:2027	acesulfame K	2016:2027	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	8	66	theme	relative	1638:1645	arg1	%					1636:1636	57, 66 and 29%	1623:1636	%	1636:1636	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	7	67	theme	subcutaneous	1372:1383	arg1	fat					1395:1397	subcutaneous abdominal fat	1372:1397	subcutaneous abdominal fat by ≈ 20%	1372:1406	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	10	68	theme	sucralose	2002:2010	arg1	active					2045:2050	active	2045:2050	active	2045:2050	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	10	68	theme	sucralose	2002:2010	arg1	combination					1987:1997	the combination	1983:1997	the combination of sucralose and acesulfame K	1983:2027	CONCLUSIONS In miniature pigs, the combination of sucralose and acesulfame K is biologically active.					
31338548	3	69	theme	insulin	741:747	arg1	sensitivity					749:759	insulin sensitivity	741:759	insulin sensitivity	741:759	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	6	70	theme	energy	1285:1290	arg1	metabolism					1292:1301	energy metabolism	1285:1301	energy metabolism	1285:1301	Body composition was also measured using CT imaging and energy metabolism quantified with indirect calorimetry.					
31338548	5	71	theme	insulin	1032:1038	arg1	sensitivity					1040:1050	insulin sensitivity	1032:1050	insulin sensitivity	1032:1050	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	2	72	theme	individual	525:534	arg1	organs					536:541	individual organs	525:541	individual organs	525:541	We hypothesized that these discrepancies are accounted for by the surrogate tools used to evaluate insulin resistance and that PET 18FDG, given its capacity to quantify insulin sensitivity in individual organs, would be more sensitive in identifying changes in glucose metabolism.					
31338548	8	73	theme	diet	1662:1665	arg1	group					1667:1671	the control diet group	1650:1671	the control diet group	1650:1671	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	1	74	theme	low-calorie	120:130	arg1	acesulfame					172:181	acesulfame	172:181	acesulfame	172:181	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	1	74	theme	low-calorie	120:130	arg1	sucralose					158:166	sucralose	158:166	sucralose	158:166	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	1	74	theme	low-calorie	120:130	arg1	LCS					144:146	LCS	144:146	LCS	144:146	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	1	74	theme	low-calorie	120:130	arg1	sweeteners					132:141	low-calorie sweeteners	120:141	low-calorie sweeteners (LCS)	120:147	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	3	75	theme	large	766:770	arg1	model					779:783	a large animal model	764:783	a large animal model of morbid obesity	764:801	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	4	76	with	mini-pigs	819:827	arg1	obesity					841:847	morbid obesity	834:847	morbid obesity	834:847	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	0	77	theme	animal	78:83	arg1	model					85:89	a large animal model	70:89	a large animal model of obesity	70:100	Low-calorie sweeteners augment tissue-specific insulin sensitivity in a large animal model of obesity.					
31338548	11	78	from	uptake	2149:2154	arg1	tissues					2168:2174	specific tissues	2159:2174	specific tissues	2159:2174	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	9	79	theme	frontal	1846:1852	arg1	cortex					1854:1859	the frontal cortex	1842:1859	the frontal cortex	1842:1859	In the brain, glucose uptake increased significantly only in the frontal cortex, associated with improved metabolic connectivity towards the hippocampus and the amygdala.					
31338548	5	80	theme	arterial	1204:1211	arg1	function					1219:1226	arterial input function	1204:1226	arterial input function	1204:1226	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	3	81	theme	morbid	788:793	arg1	obesity					795:801	morbid obesity	788:801	morbid obesity	788:801	Accordingly, we performed a comprehensive evaluation of the effects of LCS on whole-body and organ-specific glucose uptake and insulin sensitivity in a large animal model of morbid obesity.					
31338548	4	82	dep	LCS	891:893	arg1	0.5 mg/kg/day					935:947	sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day	896:947	0.5 mg/kg/day	935:947	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	82	dep	LCS	891:893	arg1	group					959:963	LCS diet group	950:963	LCS diet group	950:963	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	82	dep	LCS	891:893	arg1	1 mg/kg/day					906:916	sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day	896:947	1 mg/kg/day	906:916	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	82	dep	LCS	891:893	arg1	LCS					891:893	LCS	891:893	LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group)	891:964	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	11	83	theme	insulin	2084:2090	arg1	resistance					2092:2101	whole-body insulin resistance	2073:2101	whole-body insulin resistance	2073:2101	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	5	84	theme	function	1219:1226	arg1	measurement					1189:1199	direct measurement	1182:1199	direct measurement of arterial input function	1182:1226	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	5	85	theme	skeletal	1093:1100	arg1	muscle					1102:1107	skeletal muscle	1093:1107	skeletal muscle	1093:1107	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	5	85	theme	skeletal	1093:1100	arg1	duodenum					1076:1083	the duodenum	1072:1083	the duodenum	1072:1083	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	7	86	theme	glucose	1494:1500	arg1	uptake					1502:1507	whole-body glucose uptake	1483:1507	whole-body glucose uptake	1483:1507	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	8	87	dep	%	1745:1745	arg1	P < 0.05					1748:1755	P < 0.05	1748:1755	P < 0.05 for all	1748:1763	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	11	88	theme	specific	2159:2166	arg1	tissues					2168:2174	specific tissues	2159:2174	specific tissues	2159:2174	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	6	89	theme	indirect	1319:1326	arg1	calorimetry					1328:1338	indirect calorimetry	1319:1338	indirect calorimetry	1319:1338	Body composition was also measured using CT imaging and energy metabolism quantified with indirect calorimetry.					
31338548	4	90	theme	LCS	950:952	arg1	1 mg/kg/day					906:916	sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day	896:947	1 mg/kg/day	906:916	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	90	theme	LCS	950:952	arg1	group					959:963	LCS diet group	950:963	LCS diet group	950:963	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	8	91	dep	%	1636:1636	arg1	P < 0.05					1674:1681	P < 0.05	1674:1681	P < 0.05 for all	1674:1689	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
31338548	4	92	theme	diet	954:957	arg1	1 mg/kg/day					906:916	sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day	896:947	1 mg/kg/day	906:916	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	92	theme	diet	954:957	arg1	group					959:963	LCS diet group	950:963	LCS diet group	950:963	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	2	93	from	changes	583:589	arg1	metabolism					602:611	glucose metabolism	594:611	glucose metabolism	594:611	We hypothesized that these discrepancies are accounted for by the surrogate tools used to evaluate insulin resistance and that PET 18FDG, given its capacity to quantify insulin sensitivity in individual organs, would be more sensitive in identifying changes in glucose metabolism.					
31338548	0	94	theme	tissue-specific	31:45	arg1	sensitivity					55:65	tissue-specific insulin sensitivity	31:65	tissue-specific insulin sensitivity	31:65	Low-calorie sweeteners augment tissue-specific insulin sensitivity in a large animal model of obesity.					
31338548	5	95	theme	dynamic	1141:1147	arg1	scanning					1159:1166	dynamic PET 18FDG scanning	1141:1166	dynamic PET 18FDG scanning	1141:1166	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	7	96	theme	whole-body	1483:1492	arg1	uptake					1502:1507	whole-body glucose uptake	1483:1507	whole-body glucose uptake	1483:1507	RESULTS The LCS diet increased subcutaneous abdominal fat by ≈ 20% without causing weight gain, and reduced insulin clearance by ≈ 40%, while whole-body glucose uptake and insulin sensitivity were unchanged.					
31338548	4	97	theme	METHODS	804:810	arg1	mini-pigs					819:827	METHODS Twenty mini-pigs	804:827	METHODS Twenty mini-pigs with morbid obesity	804:847	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	2	98	theme	surrogate	399:407	arg1	tools					409:413	the surrogate tools	395:413	the surrogate tools used to evaluate insulin resistance	395:449	We hypothesized that these discrepancies are accounted for by the surrogate tools used to evaluate insulin resistance and that PET 18FDG, given its capacity to quantify insulin sensitivity in individual organs, would be more sensitive in identifying changes in glucose metabolism.					
31338548	4	99	theme	acesulfame	922:931	arg1	0.5 mg/kg/day					935:947	sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day	896:947	0.5 mg/kg/day	935:947	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	4	99	theme	acesulfame	922:931	arg1	LCS					891:893	LCS	891:893	LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group)	891:964	METHODS Twenty mini-pigs with morbid obesity were fed an obesogenic diet enriched with LCS (sucralose 1 mg/kg/day and acesulfame K 0.5 mg/kg/day, LCS diet group), or without LCS (control group), for 3 months.					
31338548	1	100	theme	human	319:323	arg1	trials					325:330	recent human trials	312:330	recent human trials	312:330	PURPOSES Whether low-calorie sweeteners (LCS), such as sucralose and acesulfame K, can alter glucose metabolism is uncertain, particularly given the inconsistent observations relating to insulin resistance in recent human trials.					
31338548	9	101	theme	metabolic	1887:1895	arg1	connectivity					1897:1908	improved metabolic connectivity	1878:1908	improved metabolic connectivity towards the hippocampus and the amygdala	1878:1949	In the brain, glucose uptake increased significantly only in the frontal cortex, associated with improved metabolic connectivity towards the hippocampus and the amygdala.					
31338548	11	102	theme	obesity	2202:2208	arg1	effects					2191:2197	the effects	2187:2197	the effects of obesity in the adipose tissue and in the brain	2187:2247	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	5	103	theme	direct	1182:1187	arg1	measurement					1189:1199	direct measurement	1182:1199	direct measurement of arterial input function	1182:1226	Glucose uptake and insulin sensitivity were determined for the duodenum, liver, skeletal muscle, adipose tissue and brain using dynamic PET 18FDG scanning together with direct measurement of arterial input function.					
31338548	11	104	theme	adipose	2217:2223	arg1	tissue					2225:2230	the adipose tissue	2213:2230	the adipose tissue	2213:2230	While not affecting whole-body insulin resistance, it increases insulin sensitivity and glucose uptake in specific tissues, mimicking the effects of obesity in the adipose tissue and in the brain.					
31338548	8	105	theme	glucose	1562:1568	arg1	uptake					1570:1575	glucose uptake	1562:1575	glucose uptake in the duodenum, liver and brain	1562:1608	In contrast, glucose uptake in the duodenum, liver and brain increased by 57, 66 and 29% relative to the control diet group (P < 0.05 for all), while insulin sensitivity increased by 53, 55 and 28% (P < 0.05 for all), respectively.					
30889765	5	0	from	cells	1336:1340	arg1	GG					1345:1346	GG	1345:1346	GG	1345:1346	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	5	1	theme	cell	1434:1437	arg1	number					1439:1444	significantly higher cell number	1413:1444	significantly higher cell number	1413:1444	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	7	2	theme	strong	1747:1752	arg1	mineralization					1754:1767	strong mineralization	1747:1767	strong mineralization in BaG OM	1747:1777	OPT and SPIM showed homogeneous 3D cell distribution with strong mineralization in BaG OM.					
30889765	8	3	theme	hASCs	1895:1899	arg1	osteogenesis					1879:1890	efficacious osteogenesis	1867:1890	efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts	1867:1963	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	4	4	theme	Plane	1078:1082	arg1	SPIM					1109:1112	SPIM	1109:1112	SPIM	1109:1112	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	4	4	theme	Plane	1078:1082	arg1	Microscopy					1097:1106	Selective Plane Illumination Microscopy	1068:1106	Selective Plane Illumination Microscopy (SPIM)	1068:1113	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	1	5	theme	glass	391:395	arg1	2-06					397:400	experimental glass 2-06	378:400	experimental glass 2-06 of composition [wt-%	378:421	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	6	theme	human	256:260	arg1	cells					275:279	human adipose stem cells	256:279	human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development	256:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	6	theme	human	256:260	arg1	hASCs					282:286	hASCs	282:286	hASCs	282:286	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	8	7	theme	3D	1904:1905	arg1	hydrogels					1907:1915	3D hydrogels	1904:1915	3D hydrogels with BaG OM with potential for bone-like grafts	1904:1963	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	4	8	theme	ECM	1244:1246	arg1	content					1219:1225	the calcium phosphate (CaP) content	1191:1225	the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL	1191:1273	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	1	9	theme	unmet	169:173	arg1	need					175:178	unmet need	169:178	unmet need for bone augmentation	169:200	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	7	10	theme	3D	1721:1722	arg1	distribution					1729:1740	homogeneous 3D cell distribution	1709:1740	homogeneous 3D cell distribution	1709:1740	OPT and SPIM showed homogeneous 3D cell distribution with strong mineralization in BaG OM.					
30889765	7	11	theme	BaG	1772:1774	arg1	OM					1776:1777	BaG OM	1772:1777	BaG OM	1772:1777	OPT and SPIM showed homogeneous 3D cell distribution with strong mineralization in BaG OM.					
30889765	3	12	theme	BaG	832:834	arg1	OM					836:837	BaG OM	832:837	BaG OM	832:837	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	1	13	theme	BaG	520:522	arg1	OM					524:525	BaG OM	520:525	BaG OM	520:525	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	13	theme	BaG	520:522	arg1	medium					512:517	osteogenic medium	501:517	osteogenic medium (BaG OM) for bone construct development	501:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	14	theme	[wt-	417:420	arg1	%					421:421	composition [wt-%	405:421	composition [wt-%	405:421	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	4	15	theme	BaG	1286:1288	arg1	OM					1290:1291	BaG OM	1286:1291	BaG OM	1286:1291	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	1	16	theme	bone	532:535	arg1	development					547:557	bone construct development	532:557	bone construct development	532:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	0	17	theme	type	133:136	arg1	hydrogels					140:148	gellan gum and collagen type I hydrogels	109:148	gellan gum and collagen type I hydrogels	109:148	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	4	18	theme	BaG	1125:1127	arg1	OM					1129:1130	BaG OM	1125:1130	BaG OM	1125:1130	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	8	19	theme	bone-like	1948:1956	arg1	grafts					1958:1963	bone-like grafts	1948:1963	bone-like grafts	1948:1963	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	1	20	theme	SiO2	476:479	arg1	Na2O					425:428	Na2O	425:428	Na2O	425:428	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	20	theme	SiO2	476:479	arg1	extract					487:493	SiO2 50.0) extract	476:493	SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development	476:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	3	21	theme	Mechanical	648:657	arg1	properties					659:668	Mechanical properties	648:668	Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels	648:715	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	1	22	theme	bioactive	361:369	arg1	glass					371:375	bioactive glass	361:375	bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development	361:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	3	23	theme	GG-BaG	691:696	arg1	properties					659:668	Mechanical properties	648:668	Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels	648:715	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	4	24	theme	calcium	1195:1201	arg1	content					1219:1225	the calcium phosphate (CaP) content	1191:1225	the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL	1191:1273	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	0	25	theme	human	68:72	arg1	cells					87:91	human adipose stem cells	68:91	human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels	68:148	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	1	26	theme	gellan	305:310	arg1	GG					317:318	GG	317:318	GG	317:318	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	26	theme	gellan	305:310	arg1	gum					312:314	gellan gum	305:314	gellan gum (GG)	305:319	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	5	27	theme	strong	1526:1531	arg1	mineralization					1533:1546	strong mineralization	1526:1546	strong mineralization	1526:1546	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	6	28	theme	GG-BaG	1637:1642	arg1	hydrogels					1644:1652	GG-BaG hydrogels	1637:1652	GG-BaG hydrogels	1637:1652	GG-BaG hydrogels rescued hASC mineralization in OM.					
30889765	3	29	theme	GG-SPD	683:688	arg1	properties					659:668	Mechanical properties	648:668	Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels	648:715	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	0	30	theme	stem	82:85	arg1	cells					87:91	human adipose stem cells	68:91	human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels	68:148	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	1	31	theme	Due	162:164	arg1	BACKGROUND					151:160	BACKGROUND	151:160	BACKGROUND Due to unmet need for bone augmentation	151:200	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	3	32	theme	hydrogels	707:715	arg1	properties					659:668	Mechanical properties	648:668	Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels	648:715	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	4	33	theme	GG-SPD	996:1001	arg1	samples					1003:1009	Hydroxyapatite-stained GG-SPD samples	973:1009	Hydroxyapatite-stained GG-SPD samples	973:1009	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	0	34	theme	gellan	109:114	arg1	gum					116:118	gellan gum	109:118	gellan gum	109:118	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	1	35	theme	collagen	324:331	arg1	COL					341:343	COL	341:343	COL	341:343	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	35	theme	collagen	324:331	arg1	type					333:336	collagen type I	324:338	collagen type I (COL)	324:344	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	36	with	hydrogels	346:354	arg1	glass					371:375	bioactive glass	361:375	bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development	361:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	4	37	from	content	1219:1225	arg1	GG-BaG					1259:1264	GG-BaG	1259:1264	GG-BaG	1259:1264	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	4	37	from	content	1219:1225	arg1	COL					1271:1273	COL	1271:1273	COL	1271:1273	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	4	37	from	content	1219:1225	arg1	GG-SPD					1251:1256	GG-SPD	1251:1256	GG-SPD	1251:1256	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	5	38	theme	BaG	1398:1400	arg1	OM					1402:1403	BaG OM	1398:1403	BaG OM	1398:1403	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	0	39	theme	Bioactive	0:8	arg1	ions					16:19	Bioactive glass ions	0:19	Bioactive glass ions	0:19	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	1	40	theme	adipose	262:268	arg1	cells					275:279	human adipose stem cells	256:279	human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development	256:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	40	theme	adipose	262:268	arg1	hASCs					282:286	hASCs	282:286	hASCs	282:286	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	3	41	theme	BaG	758:760	arg1	OM					762:763	BaG OM	758:763	BaG OM	758:763	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	8	42	theme	strong	1786:1791	arg1	production					1805:1814	strong osteocalcin production	1786:1814	strong osteocalcin production	1786:1814	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	8	43	with	hydrogels	1907:1915	arg1	BaG					1922:1924	BaG	1922:1924	BaG OM with potential for bone-like grafts	1922:1963	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	5	44	theme	improved	1605:1612	arg1	properties					1625:1634	significantly improved mechanical properties	1591:1634	significantly improved mechanical properties	1591:1634	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	0	45	theme	efficient	28:36	arg1	differentiation					49:63	efficient osteogenic differentiation	28:63	efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels	28:148	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	5	46	dep	RESULTS	1294:1300	arg1	showed					1314:1319	showed	1314:1319	showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties	1314:1634	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	8	47	theme	COL.	1831:1834	arg1	CONCLUSIONS					1836:1846	COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts	1831:1963	COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts	1831:1963	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	5	48	theme	Raman	1568:1572	arg1	spectroscopy					1574:1585	Raman spectroscopy	1568:1585	Raman spectroscopy	1568:1585	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	5	49	theme	higher	1450:1455	arg1	expression					1473:1482	higher osteogenic gene expression	1450:1482	higher osteogenic gene expression	1450:1482	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	4	50	theme	Projection	1036:1045	arg1	Tomography					1047:1056	Optical Projection Tomography	1028:1056	Optical Projection Tomography (OPT)	1028:1062	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	5	51	dep	COL.	1487:1490	arg1	induced					1518:1524	induced	1518:1524	induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties	1518:1634	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	5	52	theme	higher	1427:1432	arg1	number					1439:1444	significantly higher cell number	1413:1444	significantly higher cell number	1413:1444	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	1	53	theme	osteogenic	226:235	arg1	differentiation					237:251	osteogenic differentiation	226:251	osteogenic differentiation	226:251	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	3	54	theme	osteogenic	901:910	arg1	expression					917:926	osteogenic gene expression	901:926	osteogenic gene expression	901:926	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	8	55	theme	efficacious	1867:1877	arg1	osteogenesis					1879:1890	efficacious osteogenesis	1867:1890	efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts	1867:1963	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	1	56	dep	glass	371:375	arg1	K2O					436:438	K2O	436:438	K2O	436:438	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	56	dep	glass	371:375	arg1	2-06					397:400	experimental glass 2-06	378:400	experimental glass 2-06 of composition [wt-%	378:421	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	56	dep	glass	371:375	arg1	B2O3					466:469	B2O3	466:469	B2O3	466:469	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	56	dep	glass	371:375	arg1	extract					487:493	SiO2 50.0) extract	476:493	SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development	476:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	56	dep	glass	371:375	arg1	Na2O					425:428	Na2O	425:428	Na2O	425:428	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	56	dep	glass	371:375	arg1	CaO					446:448	CaO	446:448	CaO	446:448	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	56	dep	glass	371:375	arg1	P2O5					456:459	P2O5	456:459	P2O5	456:459	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	57	theme	experimental	378:389	arg1	2-06					397:400	experimental glass 2-06	378:400	experimental glass 2-06 of composition [wt-%	378:421	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	3	58	theme	embedded	794:801	arg1	hASCs					803:807	0, 14, and 21 d. Hydrogel embedded hASCs	768:807	0, 14, and 21 d. Hydrogel embedded hASCs	768:807	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	2	59	theme	BaG	618:620	arg1	GG-BaG					631:636	GG-BaG	631:636	GG-BaG	631:636	GG hydrogels were crosslinked with spermidine (GG-SPD) or BaG extract (GG-BaG).					
30889765	2	59	theme	BaG	618:620	arg1	extract					622:628	BaG extract	618:628	BaG extract (GG-BaG)	618:637	GG hydrogels were crosslinked with spermidine (GG-SPD) or BaG extract (GG-BaG).					
30889765	4	60	theme	Selective	1068:1076	arg1	SPIM					1109:1112	SPIM	1109:1112	SPIM	1109:1112	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	4	60	theme	Selective	1068:1076	arg1	Microscopy					1097:1106	Selective Plane Illumination Microscopy	1068:1106	Selective Plane Illumination Microscopy (SPIM)	1068:1113	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	3	61	theme	osteocalcin	929:939	arg1	production					941:950	osteocalcin production	929:950	osteocalcin production	929:950	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	8	62	from	osteogenesis	1879:1890	arg1	hydrogels					1907:1915	3D hydrogels	1904:1915	3D hydrogels with BaG OM with potential for bone-like grafts	1904:1963	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	4	63	theme	Illumination	1084:1095	arg1	SPIM					1109:1112	SPIM	1109:1112	SPIM	1109:1112	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	4	63	theme	Illumination	1084:1095	arg1	Microscopy					1097:1106	Selective Plane Illumination Microscopy	1068:1106	Selective Plane Illumination Microscopy (SPIM)	1068:1113	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	1	64	theme	composition	405:415	arg1	%					421:421	composition [wt-%	405:421	composition [wt-%	405:421	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	5	65	theme	gene	1468:1471	arg1	expression					1473:1482	higher osteogenic gene expression	1450:1482	higher osteogenic gene expression	1450:1482	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	7	66	theme	cell	1724:1727	arg1	distribution					1729:1740	homogeneous 3D cell distribution	1709:1740	homogeneous 3D cell distribution	1709:1740	OPT and SPIM showed homogeneous 3D cell distribution with strong mineralization in BaG OM.					
30889765	4	67	from	21 d.	1135:1139	arg1	OM					1118:1119	OM	1118:1119	OM	1118:1119	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	4	67	from	21 d.	1135:1139	arg1	OM					1129:1130	BaG OM	1125:1130	BaG OM	1125:1130	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	0	68	theme	collagen	124:131	arg1	type					133:136	collagen type I	124:138	collagen type I	124:138	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	4	69	from	21 d	1278:1281	arg1	OM					1290:1291	BaG OM	1286:1291	BaG OM	1286:1291	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	1	70	theme	bone	184:187	arg1	augmentation					189:200	bone augmentation	184:200	bone augmentation	184:200	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	7	71	theme	homogeneous	1709:1719	arg1	distribution					1729:1740	homogeneous 3D cell distribution	1709:1740	homogeneous 3D cell distribution	1709:1740	OPT and SPIM showed homogeneous 3D cell distribution with strong mineralization in BaG OM.					
30889765	3	72	dep	METHODS	640:646	arg1	tested					722:727	tested	722:727	were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs	717:807	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	1	73	theme	construct	537:545	arg1	development					547:557	bone construct development	532:557	bone construct development	532:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	2	74	theme	GG	560:561	arg1	hydrogels					563:571	GG hydrogels	560:571	GG hydrogels	560:571	GG hydrogels were crosslinked with spermidine (GG-SPD) or BaG extract (GG-BaG).					
30889765	1	75	theme	%	421:421	arg1	2-06					397:400	experimental glass 2-06	378:400	experimental glass 2-06 of composition [wt-%	378:421	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	5	76	theme	BaG	1511:1513	arg1	OM					1515:1516	BaG OM	1511:1516	BaG OM	1511:1516	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	3	77	theme	COL	703:705	arg1	hydrogels					707:715	COL hydrogels	703:715	COL hydrogels	703:715	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	4	78	dep	Tomography	1047:1056	arg1	OPT					1059:1061	OPT	1059:1061	OPT	1059:1061	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	5	79	dep	COL.	1380:1383	arg1	induced					1405:1411	induced	1405:1411	induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties	1405:1634	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	6	80	theme	hASC	1662:1665	arg1	mineralization					1667:1680	hASC mineralization	1662:1680	hASC mineralization	1662:1680	GG-BaG hydrogels rescued hASC mineralization in OM.					
30889765	0	81	theme	adipose	74:80	arg1	cells					87:91	human adipose stem cells	68:91	human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels	68:148	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	8	82	dep	CONCLUSIONS	1836:1846	arg1	showed					1860:1865	showed	1860:1865	showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts	1860:1963	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	0	83	theme	cells	87:91	arg1	differentiation					49:63	efficient osteogenic differentiation	28:63	efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels	28:148	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	1	84	theme	gum	312:314	arg1	hydrogels					346:354	gellan gum (GG) or collagen type I (COL) hydrogels	305:354	gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development	305:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	85	theme	osteogenic	501:510	arg1	OM					524:525	BaG OM	520:525	BaG OM	520:525	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	85	theme	osteogenic	501:510	arg1	medium					512:517	osteogenic medium	501:517	osteogenic medium (BaG OM) for bone construct development	501:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	4	86	theme	Raman	1154:1158	arg1	spectroscopy					1160:1171	Raman spectroscopy	1154:1171	Raman spectroscopy	1154:1171	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	3	87	theme	cell-free	673:681	arg1	GG-SPD					683:688	cell-free GG-SPD	673:688	cell-free GG-SPD	673:688	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	0	88	theme	gum	116:118	arg1	hydrogels					140:148	gellan gum and collagen type I hydrogels	109:148	gellan gum and collagen type I hydrogels	109:148	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	8	89	from	CONCLUSIONS	1836:1846	arg1	visible					1820:1826	visible	1820:1826	visible	1820:1826	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	0	90	theme	glass	10:14	arg1	ions					16:19	Bioactive glass ions	0:19	Bioactive glass ions	0:19	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	1	91	theme	type	333:336	arg1	hydrogels					346:354	gellan gum (GG) or collagen type I (COL) hydrogels	305:354	gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development	305:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	8	92	theme	osteocalcin	1793:1803	arg1	production					1805:1814	strong osteocalcin production	1786:1814	strong osteocalcin production	1786:1814	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	4	93	theme	Hydroxyapatite-stained	973:994	arg1	samples					1003:1009	Hydroxyapatite-stained GG-SPD samples	973:1009	Hydroxyapatite-stained GG-SPD samples	973:1009	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	4	94	theme	hASC-secreted	1230:1242	arg1	ECM					1244:1246	hASC-secreted ECM	1230:1246	hASC-secreted ECM	1230:1246	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	1	95	theme	stem	270:273	arg1	cells					275:279	human adipose stem cells	256:279	human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development	256:557	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	1	95	theme	stem	270:273	arg1	hASCs					282:286	hASCs	282:286	hASCs	282:286	BACKGROUND Due to unmet need for bone augmentation, our aim was to promote osteogenic differentiation of human adipose stem cells (hASCs) encapsulated in gellan gum (GG) or collagen type I (COL) hydrogels with bioactive glass (experimental glass 2-06 of composition [wt-%]: Na2O 12.1, K2O 14.0, CaO 19.8, P2O5 2.5, B2O3 1.6, SiO2 50.0) extract based osteogenic medium (BaG OM) for bone construct development.					
30889765	5	96	dep	induced	1518:1524	arg1	confirmed					1548:1556	confirmed	1548:1556	induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties	1518:1634	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	4	97	theme	phosphate	1203:1211	arg1	content					1219:1225	the calcium phosphate (CaP) content	1191:1225	the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL	1191:1273	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	0	98	theme	osteogenic	38:47	arg1	differentiation					49:63	efficient osteogenic differentiation	28:63	efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels	28:148	Bioactive glass ions induce efficient osteogenic differentiation of human adipose stem cells encapsulated in gellan gum and collagen type I hydrogels.					
30889765	5	99	theme	viable	1321:1326	arg1	cells					1336:1340	viable rounded cells	1321:1340	viable rounded cells in GG	1321:1346	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	5	100	theme	mechanical	1614:1623	arg1	properties					1625:1634	significantly improved mechanical properties	1591:1634	significantly improved mechanical properties	1591:1634	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	4	101	theme	CaP	1214:1216	arg1	content					1219:1225	the calcium phosphate (CaP) content	1191:1225	the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL	1191:1273	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	3	102	theme	cell	888:891	arg1	number					893:898	cell number	888:898	cell number	888:898	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	7	103	from	mineralization	1754:1767	arg1	OM					1776:1777	BaG OM	1772:1777	BaG OM	1772:1777	OPT and SPIM showed homogeneous 3D cell distribution with strong mineralization in BaG OM.					
30889765	4	104	used	used	1177:1180	arg2	spectroscopy					1160:1171	Raman spectroscopy	1154:1171	Raman spectroscopy	1154:1171	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	4	105	theme	Optical	1028:1034	arg1	Tomography					1047:1056	Optical Projection Tomography	1028:1056	Optical Projection Tomography (OPT)	1028:1062	Hydroxyapatite-stained GG-SPD samples were imaged with Optical Projection Tomography (OPT) and Selective Plane Illumination Microscopy (SPIM) in OM and BaG OM at 21 d. Furthermore, Raman spectroscopy was used to study the calcium phosphate (CaP) content of hASC-secreted ECM in GG-SPD, GG-BaG, and COL at 21 d in BaG OM.					
30889765	5	106	theme	osteogenic	1457:1466	arg1	expression					1473:1482	higher osteogenic gene expression	1450:1482	higher osteogenic gene expression	1450:1482	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30889765	3	107	theme	osteogenic	732:741	arg1	OM					751:752	OM	751:752	OM	751:752	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	3	107	theme	osteogenic	732:741	arg1	medium					743:748	osteogenic medium	732:748	osteogenic medium (OM)	732:753	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	3	108	theme	gene	912:915	arg1	expression					917:926	osteogenic gene expression	901:926	osteogenic gene expression	901:926	METHODS Mechanical properties of cell-free GG-SPD, GG-BaG, and COL hydrogels were tested in osteogenic medium (OM) or BaG OM at 0, 14, and 21 d. Hydrogel embedded hASCs were cultured in OM or BaG OM for 3, 14, and 21 d, and analyzed for viability, cell number, osteogenic gene expression, osteocalcin production, and mineralization.					
30889765	8	109	from	visible	1820:1826	arg1	CONCLUSIONS					1836:1846	COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts	1831:1963	COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts	1831:1963	Also, strong osteocalcin production was visible in COL. CONCLUSIONS Overall, we showed efficacious osteogenesis of hASCs in 3D hydrogels with BaG OM with potential for bone-like grafts.					
30889765	5	110	theme	rounded	1328:1334	arg1	cells					1336:1340	viable rounded cells	1321:1340	viable rounded cells in GG	1321:1346	RESULTS The results showed viable rounded cells in GG whereas hASCs were elongated in COL. Importantly, BaG OM induced significantly higher cell number and higher osteogenic gene expression in COL. In both hydrogels, BaG OM induced strong mineralization confirmed as CaP by Raman spectroscopy and significantly improved mechanical properties.					
30704065	0	0	theme	Intestinal	79:88	arg1	Microbiota					90:99	the Intestinal Microbiota	75:99	the Intestinal Microbiota	75:99	Resistant Starch Attenuates Bone Loss in Ovariectomised Mice by Regulating the Intestinal Microbiota and Bone-Marrow Inflammation.					
30704065	10	1	theme	attenuated	1367:1376	arg1	resorption					1383:1392	attenuated bone resorption	1367:1392	attenuated bone resorption in OVX mice	1367:1404	These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.					
30704065	4	2	theme	acid-hydrolysed	762:776	arg1	diet					791:794	a 20% acid-hydrolysed HAS (AH-HAS) diet	756:794	a 20% acid-hydrolysed HAS (AH-HAS) diet	756:794	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	10	3	theme	bone	1341:1344	arg1	marrow					1346:1351	the bone marrow	1337:1351	the bone marrow	1337:1351	These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.					
30704065	1	4	theme	bone	170:173	arg1	metabolism					175:184	bone metabolism	170:184	bone metabolism	170:184	The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported.					
30704065	4	5	theme	high	707:710	arg1	diet					738:741	a 20% high amylose corn starch (HAS) diet	701:741	a 20% high amylose corn starch (HAS) diet	701:741	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	8	6	theme	IL-7	1142:1145	arg1	receptor					1147:1154	IL-7 receptor	1142:1154	IL-7 receptor	1142:1154	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	4	7	theme	%	760:760	arg1	diet					791:794	a 20% acid-hydrolysed HAS (AH-HAS) diet	756:794	a 20% acid-hydrolysed HAS (AH-HAS) diet	756:794	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	3	8	theme	intestinal	482:491	arg1	composition					503:513	intestinal microbial composition	482:513	intestinal microbial composition	482:513	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	5	9	theme	RS	844:845	arg1	%					831:831	6.8%	828:831	6.8%	828:831	HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively.					
30704065	5	9	theme	RS	844:845	arg1	%					839:839	12%	837:839	12% of RS	837:845	HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively.					
30704065	5	9	theme	RS	844:845	arg1	RS					844:845	RS	844:845	RS	844:845	HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively.					
30704065	6	10	theme	spp	949:951	arg1	abundance					920:928	the abundance	916:928	the abundance of Bifidobacterium spp	916:951	After 6 weeks, treatment with HAS or AH-HAS increased the abundance of Bifidobacterium spp.					
30704065	4	11	theme	corn	720:723	arg1	starch					725:730	amylose corn starch	712:730	a 20% high amylose corn starch (HAS) diet	701:741	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	3	12	theme	mineral	521:527	arg1	density					529:535	bone mineral density	516:535	bone mineral density	516:535	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	3	13	from	density	529:535	arg1	composition					503:513	intestinal microbial composition	482:513	intestinal microbial composition	482:513	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	1	14	theme	oestrogen-deficient	267:285	arg1	mice					287:290	oestrogen-deficient mice	267:290	oestrogen-deficient mice	267:290	The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported.					
30704065	4	15	dep	groups	672:677	arg1	OVX					748:750	OVX	748:750	OVX	748:750	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	4	15	dep	groups	672:677	arg1	OVX					693:695	OVX	693:695	OVX fed a 20% high amylose corn starch (HAS) diet	693:741	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	4	15	dep	groups	672:677	arg1	groups					672:677	three groups	666:677	three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet	666:794	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	4	15	dep	groups	672:677	arg1	control					684:690	OVX control	680:690	OVX control	680:690	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	4	16	theme	20	703:704	arg1	%					705:705	%	705:705	%	705:705	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	1	17	theme	mice	287:290	arg1	tissues					256:262	bone tissues	251:262	bone tissues of oestrogen-deficient mice	251:290	The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported.					
30704065	8	18	theme	interleukin	1021:1031	arg1	IL					1034:1035	interleukin (IL)-10	1021:1039	interleukin (IL)-10	1021:1039	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	8	19	theme	receptor	1086:1093	arg1	activator					1095:1103	receptor activator	1086:1103	receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes	1086:1160	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	4	20	theme	amylose	712:718	arg1	starch					725:730	amylose corn starch	712:730	a 20% high amylose corn starch (HAS) diet	701:741	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	4	21	dep	starch	725:730	arg1	HAS					733:735	HAS	733:735	HAS	733:735	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	4	22	theme	OVX	630:632	arg1	mice					634:637	OVX mice	630:637	OVX mice	630:637	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	0	23	theme	Bone-Marrow	105:115	arg1	Inflammation					117:128	Bone-Marrow Inflammation	105:128	Bone-Marrow Inflammation	105:128	Resistant Starch Attenuates Bone Loss in Ovariectomised Mice by Regulating the Intestinal Microbiota and Bone-Marrow Inflammation.					
30704065	3	24	theme	bone	516:519	arg1	density					529:535	bone mineral density	516:535	bone mineral density	516:535	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	6	25	theme	Bifidobacterium	933:947	arg1	spp					949:951	Bifidobacterium spp	933:951	Bifidobacterium spp	933:951	After 6 weeks, treatment with HAS or AH-HAS increased the abundance of Bifidobacterium spp.					
30704065	10	26	theme	OVX	1397:1399	arg1	mice					1401:1404	OVX mice	1397:1404	OVX mice	1397:1404	These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.					
30704065	8	27	theme	IL	1034:1035	arg1	expression					1007:1016	mRNA expression	1002:1016	mRNA expression of interleukin (IL)-10 in the colon	1002:1052	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	0	28	from	Loss	33:36	arg1	Mice					56:59	Ovariectomised Mice	41:59	Ovariectomised Mice	41:59	Resistant Starch Attenuates Bone Loss in Ovariectomised Mice by Regulating the Intestinal Microbiota and Bone-Marrow Inflammation.					
30704065	4	29	theme	20	758:759	arg1	%					760:760	%	760:760	%	760:760	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	3	30	theme	inflammatory-gene	542:558	arg1	expression					560:569	inflammatory-gene expression	542:569	inflammatory-gene expression	542:569	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	3	31	dep	colon	578:582	arg1	the					574:576	the	574:576	the	574:576	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	0	32	theme	Resistant	0:8	arg1	Starch					10:15	Resistant Starch	0:15	Resistant Starch	0:15	Resistant Starch Attenuates Bone Loss in Ovariectomised Mice by Regulating the Intestinal Microbiota and Bone-Marrow Inflammation.					
30704065	8	33	theme	OVX	1184:1186	arg1	mice					1188:1191	OVX mice	1184:1191	OVX mice	1184:1191	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	4	34	theme	%	705:705	arg1	diet					738:741	a 20% high amylose corn starch (HAS) diet	701:741	a 20% high amylose corn starch (HAS) diet	701:741	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	0	35	theme	Bone	28:31	arg1	Loss					33:36	Bone Loss	28:36	Bone Loss in Ovariectomised Mice	28:59	Resistant Starch Attenuates Bone Loss in Ovariectomised Mice by Regulating the Intestinal Microbiota and Bone-Marrow Inflammation.					
30704065	8	36	theme	ligand	1131:1136	arg1	genes					1156:1160	nuclear factor kappa-B ligand and IL-7 receptor genes	1108:1160	nuclear factor kappa-B ligand and IL-7 receptor genes	1108:1160	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	8	37	theme	genes	1156:1160	arg1	activator					1095:1103	receptor activator	1086:1103	receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes	1086:1160	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	1	38	theme	pro-inflammatory	208:223	arg1	cytokines					225:233	pro-inflammatory cytokines	208:233	pro-inflammatory cytokines	208:233	The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported.					
30704065	2	39	theme	gut	410:412	arg1	microbiota					414:423	the gut microbiota	406:423	the gut microbiota	406:423	Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota.					
30704065	5	40	dep	HAS	797:799	arg1	diets					812:816	diets	812:816	diets	812:816	HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively.					
30704065	2	41	theme	Resistant	312:320	arg1	starch					322:327	Resistant starch	312:327	Resistant starch (RS)	312:332	Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota.					
30704065	2	41	theme	Resistant	312:320	arg1	type					339:342	a type	337:342	a type of dietary fibre	337:359	Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota.					
30704065	2	41	theme	Resistant	312:320	arg1	RS					330:331	RS	330:331	RS	330:331	Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota.					
30704065	8	42	theme	mice	1188:1191	arg1	marrow					1174:1179	the bone marrow	1165:1179	the bone marrow of OVX mice	1165:1191	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	1	43	theme	cytokines	225:233	arg1	levels					198:203	levels	198:203	levels of pro-inflammatory cytokines	198:233	The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported.					
30704065	3	44	theme	mice	624:627	arg1	colon					578:582	colon	578:582	colon	578:582	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	3	44	theme	mice	624:627	arg1	marrow					593:598	bone marrow	588:598	bone marrow	588:598	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	8	45	theme	receptor	1147:1154	arg1	genes					1156:1160	nuclear factor kappa-B ligand and IL-7 receptor genes	1108:1160	nuclear factor kappa-B ligand and IL-7 receptor genes	1108:1160	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	5	46	contain	contained	818:826	arg2	RS					844:845	RS	844:845	RS	844:845	HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively.					
30704065	5	46	contain	contained	818:826	arg2	%					839:839	12%	837:839	12% of RS	837:845	HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively.					
30704065	5	46	contain	contained	818:826	arg1	AH-HAS					805:810	AH-HAS	805:810	AH-HAS	805:810	HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively.					
30704065	5	46	contain	contained	818:826	arg1	HAS					797:799	HAS	797:799	HAS	797:799	HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively.					
30704065	5	46	contain	contained	818:826	arg2	%					831:831	6.8%	828:831	6.8%	828:831	HAS and AH-HAS diets contained 6.8% and 12% of RS, respectively.					
30704065	8	47	theme	nuclear	1108:1114	arg1	factor					1116:1121	nuclear factor	1108:1121	nuclear factor kappa-B ligand	1108:1136	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	10	48	theme	immune	1320:1325	arg1	status					1327:1332	immune status	1320:1332	immune status	1320:1332	These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.					
30704065	10	49	from	resorption	1383:1392	arg1	mice					1401:1404	OVX mice	1397:1404	OVX mice	1397:1404	These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.					
30704065	2	50	theme	microbiota	414:423	arg1	composition					391:401	the composition	387:401	the composition of the gut microbiota	387:423	Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota.					
30704065	3	51	dep	ovariectomised	603:616	arg1	OVX					619:621	OVX	619:621	OVX	619:621	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	4	52	dep	acid-hydrolysed	762:776	arg1	HAS					778:780	HAS	778:780	HAS	778:780	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	8	53	theme	bone	1169:1172	arg1	marrow					1174:1179	the bone marrow	1165:1179	the bone marrow of OVX mice	1165:1191	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	6	54	with	treatment	877:885	arg1	AH-HAS					899:904	AH-HAS	899:904	AH-HAS	899:904	After 6 weeks, treatment with HAS or AH-HAS increased the abundance of Bifidobacterium spp.					
30704065	6	54	with	treatment	877:885	arg1	HAS					892:894	HAS	892:894	HAS	892:894	After 6 weeks, treatment with HAS or AH-HAS increased the abundance of Bifidobacterium spp.					
30704065	8	55	from	expression	1072:1081	arg1	marrow					1174:1179	the bone marrow	1165:1179	the bone marrow of OVX mice	1165:1191	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	8	56	theme	kappa-B	1123:1129	arg1	ligand					1131:1136	nuclear factor kappa-B ligand	1108:1136	nuclear factor kappa-B ligand	1108:1136	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	0	57	theme	Ovariectomised	41:54	arg1	Mice					56:59	Ovariectomised Mice	41:59	Ovariectomised Mice	41:59	Resistant Starch Attenuates Bone Loss in Ovariectomised Mice by Regulating the Intestinal Microbiota and Bone-Marrow Inflammation.					
30704065	10	58	dep	microbiota	1305:1314	arg1	the					1301:1303	the	1301:1303	the	1301:1303	These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.					
30704065	3	59	from	effects	443:449	arg1	composition					503:513	intestinal microbial composition	482:513	intestinal microbial composition	482:513	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	8	60	theme	factor	1116:1121	arg1	ligand					1131:1136	nuclear factor kappa-B ligand	1108:1136	nuclear factor kappa-B ligand	1108:1136	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	9	61	theme	AH-HAS	1194:1199	arg1	treatment					1201:1209	AH-HAS treatment	1194:1209	AH-HAS treatment	1194:1209	AH-HAS treatment attenuated ovariectomy-induced bone loss.					
30704065	8	62	theme	AH-HAS	969:974	arg1	diet					976:979	The AH-HAS diet	965:979	The AH-HAS diet	965:979	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	8	63	theme	mRNA	1002:1005	arg1	expression					1007:1016	mRNA expression	1002:1016	mRNA expression of interleukin (IL)-10 in the colon	1002:1052	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	4	64	theme	starch	725:730	arg1	diet					738:741	a 20% high amylose corn starch (HAS) diet	701:741	a 20% high amylose corn starch (HAS) diet	701:741	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	2	65	from	changes	376:382	arg1	composition					391:401	the composition	387:401	the composition of the gut microbiota	387:423	Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota.					
30704065	1	66	theme	intestinal	135:144	arg1	microbiota					146:155	The intestinal microbiota	131:155	The intestinal microbiota	131:155	The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported.					
30704065	10	67	theme	marrow	1346:1351	arg1	microbiota					1305:1314	microbiota	1305:1314	microbiota	1305:1314	These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.					
30704065	10	67	theme	marrow	1346:1351	arg1	status					1327:1332	immune status	1320:1332	immune status	1320:1332	These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.					
30704065	1	68	theme	T	240:240	arg1	cells					242:246	T cells	240:246	T cells in bone tissues of oestrogen-deficient mice	240:290	The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported.					
30704065	1	69	from	cells	242:246	arg1	tissues					256:262	bone tissues	251:262	bone tissues of oestrogen-deficient mice	251:290	The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported.					
30704065	8	70	from	expression	1007:1016	arg1	colon					1048:1052	the colon	1044:1052	the colon	1044:1052	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	3	71	theme	ovariectomised	603:616	arg1	mice					624:627	ovariectomised (OVX) mice	603:627	ovariectomised (OVX) mice	603:627	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	4	72	theme	OVX	680:682	arg1	groups					672:677	three groups	666:677	three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet	666:794	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	4	72	theme	OVX	680:682	arg1	control					684:690	OVX control	680:690	OVX control	680:690	OVX mice were divided randomly into three groups: OVX control, OVX fed a 20% high amylose corn starch (HAS) diet, and OVX fed a 20% acid-hydrolysed HAS (AH-HAS) diet.					
30704065	3	73	theme	RS	454:455	arg1	density					529:535	bone mineral density	516:535	bone mineral density	516:535	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	3	73	theme	RS	454:455	arg1	effects					443:449	the effects	439:449	the effects of RS supplemented in diets on intestinal microbial composition	439:513	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	3	73	theme	RS	454:455	arg1	expression					560:569	inflammatory-gene expression	542:569	inflammatory-gene expression	542:569	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	3	74	theme	bone	588:591	arg1	marrow					593:598	bone marrow	588:598	bone marrow	588:598	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	8	75	theme	activator	1095:1103	arg1	expression					1072:1081	expression	1072:1081	expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice	1072:1191	The AH-HAS diet tended to upregulate mRNA expression of interleukin (IL)-10 in the colon, and downregulate expression of receptor activator of nuclear factor kappa-B ligand and IL-7 receptor genes in the bone marrow of OVX mice.					
30704065	3	76	theme	microbial	493:501	arg1	composition					503:513	intestinal microbial composition	482:513	intestinal microbial composition	482:513	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	3	77	from	expression	560:569	arg1	composition					503:513	intestinal microbial composition	482:513	intestinal microbial composition	482:513	We evaluated the effects of RS supplemented in diets on intestinal microbial composition, bone mineral density, and inflammatory-gene expression in the colon and bone marrow of ovariectomised (OVX) mice.					
30704065	9	78	theme	ovariectomy-induced	1222:1240	arg1	loss					1247:1250	ovariectomy-induced bone loss	1222:1250	ovariectomy-induced bone loss	1222:1250	AH-HAS treatment attenuated ovariectomy-induced bone loss.					
30704065	10	79	theme	bone	1378:1381	arg1	resorption					1383:1392	attenuated bone resorption	1367:1392	attenuated bone resorption in OVX mice	1367:1404	These findings suggest that AH-HAS might change the microbiota and immune status of the bone marrow, resulting in attenuated bone resorption in OVX mice.					
30704065	9	80	theme	bone	1242:1245	arg1	loss					1247:1250	ovariectomy-induced bone loss	1222:1250	ovariectomy-induced bone loss	1222:1250	AH-HAS treatment attenuated ovariectomy-induced bone loss.					
30704065	2	81	theme	fibre	355:359	arg1	starch					322:327	Resistant starch	312:327	Resistant starch (RS)	312:332	Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota.					
30704065	2	81	theme	fibre	355:359	arg1	type					339:342	a type	337:342	a type of dietary fibre	337:359	Resistant starch (RS) is a type of dietary fibre and results in changes in the composition of the gut microbiota.					
30704065	1	82	theme	bone	251:254	arg1	tissues					256:262	bone tissues	251:262	bone tissues of oestrogen-deficient mice	251:290	The intestinal microbiota may regulate bone metabolism by reducing levels of pro-inflammatory cytokines, and T cells in bone tissues of oestrogen-deficient mice have been reported.					
31571874	6	0	theme	spherical	1141:1149	arg1	nanoparticles					1151:1163	spherical nanoparticles	1141:1163	spherical nanoparticles	1141:1163	Scanning electron microscopy was used to observe CSAD-VB12/insulin as spherical nanoparticles.					
31571874	6	0	theme	spherical	1141:1149	arg1	CSAD-VB12/insulin					1120:1136	CSAD-VB12/insulin	1120:1136	CSAD-VB12/insulin	1120:1136	Scanning electron microscopy was used to observe CSAD-VB12/insulin as spherical nanoparticles.					
31571874	11	1	theme	nm	1711:1712	arg1	size					1697:1700	the size	1693:1700	the size of 30-50 nm	1693:1712	RESULTS They were observed as spherical nanoparticles in the size of 30-50 nm.					
31571874	3	2	theme	peptide	524:530	arg1	drugs					532:536	peptide drugs	524:536	peptide drugs	524:536	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	1	3	used	used	169:172	arg2	drugs					153:157	PURPOSE Peptide drugs	137:157	PURPOSE Peptide drugs	137:157	PURPOSE Peptide drugs have been used in therapy various diseases.					
31571874	13	4	theme	CSAD/insulin	1885:1896	arg1	nanoparticles					1898:1910	the CSAD/insulin nanoparticles	1881:1910	the CSAD/insulin nanoparticles	1881:1910	Comparing with insulin and the CSAD/insulin nanoparticles, the CSAD-VB12/insulin nanoparticles exhibited higher permeation ability through intestinal enterocytes in the Caco-2 cell model.					
31571874	9	5	theme	intestinal	1499:1508	arg1	enterocytes					1510:1520	intestinal enterocytes	1499:1520	intestinal enterocytes	1499:1520	Furthermore, confocal was used to confirm the endocytosis of intestinal enterocytes.					
31571874	9	6	used	used	1464:1467	arg2	confocal					1451:1458	confocal	1451:1458	confocal	1451:1458	Furthermore, confocal was used to confirm the endocytosis of intestinal enterocytes.					
31571874	4	7	theme	NMR	884:886	arg1	spectroscopy					888:899	FTIR and 1H NMR spectroscopy	872:899	spectroscopy	888:899	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	8	8	theme	Caco-2	1319:1324	arg1	model					1331:1335	Caco-2 cell model	1319:1335	Caco-2 cell model	1319:1335	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	1	9	theme	therapy	177:183	arg1	diseases					193:200	therapy various diseases	177:200	therapy various diseases	177:200	PURPOSE Peptide drugs have been used in therapy various diseases.					
31571874	12	10	theme	Caco-2	1839:1844	arg1	cells					1847:1851	the human colon adenocarcinoma (Caco-2) cells	1807:1851	the human colon adenocarcinoma (Caco-2) cells	1807:1851	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells.					
31571874	4	11	theme	sodium	715:720	arg1	derivative					731:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	5	12	with	nanoparticles	996:1008	arg1	potentials					1029:1038	negative zeta potentials	1015:1038	negative zeta potentials	1015:1038	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	11	13	located	observed	1654:1661	arg1	size					1697:1700	the size	1693:1700	the size of 30-50 nm	1693:1712	RESULTS They were observed as spherical nanoparticles in the size of 30-50 nm.					
31571874	11	13	located	observed	1654:1661	arg2	RESULTS					1636:1642	RESULTS	1636:1642	RESULTS They	1636:1647	RESULTS They were observed as spherical nanoparticles in the size of 30-50 nm.					
31571874	11	13	located	observed	1654:1661	arg2	nanoparticles					1676:1688	spherical nanoparticles	1666:1688	spherical nanoparticles	1666:1688	RESULTS They were observed as spherical nanoparticles in the size of 30-50 nm.					
31571874	5	14	used	used	914:917	arg2	drug					938:941	a model peptide drug	922:941	a model peptide drug	922:941	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	5	14	used	used	914:917	arg2	Insulin					902:908	Insulin	902:908	Insulin	902:908	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	14	15	theme	Oral	2042:2045	arg1	administration					2047:2060	Oral administration	2042:2060	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice	2042:2123	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice yields higher intestinal retention effect, targeted absorption, and outstanding efficacy.					
31571874	15	16	dep	prospective	2434:2444	arg1	clinical					2446:2453	clinical	2446:2453	clinical	2446:2453	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	14	17	theme	retention	2150:2158	arg1	effect					2160:2165	higher intestinal retention effect	2132:2165	higher intestinal retention effect	2132:2165	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice yields higher intestinal retention effect, targeted absorption, and outstanding efficacy.					
31571874	0	18	theme	Drugs	130:134	arg1	Efficacy					110:117	The Oral Delivery Efficacy	92:117	The Oral Delivery Efficacy Of Peptide Drugs	92:134	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	8	19	used	used	1341:1344	arg2	model					1331:1335	Caco-2 cell model	1319:1335	Caco-2 cell model	1319:1335	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	8	20	theme	apparent	1361:1368	arg1	permeability					1370:1381	the apparent permeability	1357:1381	the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin	1357:1435	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	8	20	theme	apparent	1361:1368	arg1	Papp					1384:1387	Papp	1384:1387	Papp	1384:1387	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	2	21	theme	poor	216:219	arg1	bioavailability					221:235	the poor bioavailability	212:235	the poor bioavailability of peptide drugs for oral administration	212:276	However, the poor bioavailability of peptide drugs for oral administration has limited their clinical applications, on account of the acidic environment and digestive enzymes inside the human gastrointestinal tract.					
31571874	15	22	theme	oral	2405:2408	arg1	delivery					2418:2425	oral peptide delivery	2405:2425	oral peptide delivery	2405:2425	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	12	23	theme	colon	1817:1821	arg1	cells					1847:1851	the human colon adenocarcinoma (Caco-2) cells	1807:1851	the human colon adenocarcinoma (Caco-2) cells	1807:1851	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells.					
31571874	2	24	theme	oral	258:261	arg1	administration					263:276	oral administration	258:276	oral administration	258:276	However, the poor bioavailability of peptide drugs for oral administration has limited their clinical applications, on account of the acidic environment and digestive enzymes inside the human gastrointestinal tract.					
31571874	5	25	theme	CSAD-VB12	966:974	arg1	nanoparticles					996:1008	the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles	947:1008	the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials	947:1038	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	1	26	theme	PURPOSE	137:143	arg1	drugs					153:157	PURPOSE Peptide drugs	137:157	PURPOSE Peptide drugs	137:157	PURPOSE Peptide drugs have been used in therapy various diseases.					
31571874	2	27	theme	drugs	248:252	arg1	bioavailability					221:235	the poor bioavailability	212:235	the poor bioavailability of peptide drugs for oral administration	212:276	However, the poor bioavailability of peptide drugs for oral administration has limited their clinical applications, on account of the acidic environment and digestive enzymes inside the human gastrointestinal tract.					
31571874	5	28	theme	model	924:928	arg1	drug					938:941	a model peptide drug	922:941	a model peptide drug	922:941	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	5	28	theme	model	924:928	arg1	Insulin					902:908	Insulin	902:908	Insulin	902:908	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	8	29	theme	insulin	1393:1399	arg1	permeability					1370:1381	the apparent permeability	1357:1381	the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin	1357:1435	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	8	29	theme	insulin	1393:1399	arg1	Papp					1384:1387	Papp	1384:1387	Papp	1384:1387	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	13	30	theme	cell	2030:2033	arg1	model					2035:2039	the Caco-2 cell model	2019:2039	the Caco-2 cell model	2019:2039	Comparing with insulin and the CSAD/insulin nanoparticles, the CSAD-VB12/insulin nanoparticles exhibited higher permeation ability through intestinal enterocytes in the Caco-2 cell model.					
31571874	8	31	theme	CSAD/insulin	1402:1413	arg1	permeability					1370:1381	the apparent permeability	1357:1381	the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin	1357:1435	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	8	31	theme	CSAD/insulin	1402:1413	arg1	Papp					1384:1387	Papp	1384:1387	Papp	1384:1387	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	0	32	theme	Delivery	101:108	arg1	Efficacy					110:117	The Oral Delivery Efficacy	92:117	The Oral Delivery Efficacy Of Peptide Drugs	92:134	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	5	33	theme	negative	1015:1022	arg1	potentials					1029:1038	negative zeta potentials	1015:1038	negative zeta potentials	1015:1038	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	1	34	theme	Peptide	145:151	arg1	drugs					153:157	PURPOSE Peptide drugs	137:157	PURPOSE Peptide drugs	137:157	PURPOSE Peptide drugs have been used in therapy various diseases.					
31571874	8	35	theme	CSAD-VB12/insulin	1419:1435	arg1	permeability					1370:1381	the apparent permeability	1357:1381	the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin	1357:1435	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	8	35	theme	CSAD-VB12/insulin	1419:1435	arg1	Papp					1384:1387	Papp	1384:1387	Papp	1384:1387	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	3	36	theme	human	447:451	arg1	tract					470:474	the human gastrointestinal tract	443:474	the human gastrointestinal tract	443:474	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	6	37	used	used	1104:1107	arg2	microscopy					1089:1098	Scanning electron microscopy	1071:1098	Scanning electron microscopy	1071:1098	Scanning electron microscopy was used to observe CSAD-VB12/insulin as spherical nanoparticles.					
31571874	15	38	theme	CSAD-VB12	2226:2234	arg1	derivatives					2236:2246	CONCLUSION CSAD-VB12 derivatives	2215:2246	CONCLUSION CSAD-VB12 derivatives	2215:2246	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	15	39	from	candidate	2391:2399	arg1	application					2455:2465	the prospective clinical application	2430:2465	the prospective clinical application	2430:2465	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	4	40	theme	N	778:778	arg1	method					815:820	the N,N'-dicyclohexylcarbodiimide active method	774:820	method	815:820	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	2	41	theme	human	389:393	arg1	tract					412:416	the human gastrointestinal tract	385:416	the human gastrointestinal tract	385:416	However, the poor bioavailability of peptide drugs for oral administration has limited their clinical applications, on account of the acidic environment and digestive enzymes inside the human gastrointestinal tract.					
31571874	7	42	theme	colon	1268:1272	arg1	cells					1298:1302	the human colon adenocarcinoma (Caco-2) cells	1258:1302	the human colon adenocarcinoma (Caco-2) cells	1258:1302	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells by CCK-8 test.					
31571874	5	43	theme	CSAD-VB12/insulin	977:993	arg1	nanoparticles					996:1008	the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles	947:1008	the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials	947:1038	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	10	44	theme	intestinal	1570:1579	arg1	absorption					1581:1590	intestinal absorption	1570:1590	intestinal absorption	1570:1590	Type 1 diabetes mice were used to evaluate the intestinal absorption and retention effect of test nanoparticles.					
31571874	4	45	theme	N'-dicyclohexylcarbodiimide	780:806	arg1	method					815:820	the N,N'-dicyclohexylcarbodiimide active method	774:820	method	815:820	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	13	46	theme	permeation	1966:1975	arg1	ability					1977:1983	higher permeation ability	1959:1983	higher permeation ability	1959:1983	Comparing with insulin and the CSAD/insulin nanoparticles, the CSAD-VB12/insulin nanoparticles exhibited higher permeation ability through intestinal enterocytes in the Caco-2 cell model.					
31571874	0	47	theme	Vitamin	17:23	arg1	Derivatives					66:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	15	48	theme	prospective	2434:2444	arg1	application					2455:2465	the prospective clinical application	2430:2465	the prospective clinical application	2430:2465	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	14	49	theme	higher	2132:2137	arg1	effect					2160:2165	higher intestinal retention effect	2132:2165	higher intestinal retention effect	2132:2165	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice yields higher intestinal retention effect, targeted absorption, and outstanding efficacy.					
31571874	4	50	theme	B12-modified	690:701	arg1	derivative					731:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	0	51	theme	Amphiphilic	38:48	arg1	Derivatives					66:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	6	52	theme	Scanning	1071:1078	arg1	microscopy					1089:1098	Scanning electron microscopy	1071:1098	Scanning electron microscopy	1071:1098	Scanning electron microscopy was used to observe CSAD-VB12/insulin as spherical nanoparticles.					
31571874	3	53	theme	drug	507:510	arg1	delivery					512:519	targeted drug delivery	498:519	targeted drug delivery of peptide drugs	498:536	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	0	54	theme	Alginate	57:64	arg1	Derivatives					66:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	15	55	theme	peptide	2410:2416	arg1	delivery					2418:2425	oral peptide delivery	2405:2425	oral peptide delivery	2405:2425	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	4	56	dep	MATERIALS	658:666	arg1	derivative					731:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	14	57	theme	outstanding	2193:2203	arg1	efficacy					2205:2212	outstanding efficacy	2193:2212	outstanding efficacy	2193:2212	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice yields higher intestinal retention effect, targeted absorption, and outstanding efficacy.					
31571874	11	58	theme	spherical	1666:1674	arg1	nanoparticles					1676:1688	spherical nanoparticles	1666:1688	spherical nanoparticles	1666:1688	RESULTS They were observed as spherical nanoparticles in the size of 30-50 nm.					
31571874	11	58	theme	spherical	1666:1674	arg1	RESULTS					1636:1642	RESULTS	1636:1642	RESULTS They	1636:1647	RESULTS They were observed as spherical nanoparticles in the size of 30-50 nm.					
31571874	15	59	dep	absorption	2277:2286	arg1	efficacy					2288:2295	efficacy	2288:2295	efficacy	2288:2295	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	3	60	theme	drugs	532:536	arg1	delivery					512:519	targeted drug delivery	498:519	targeted drug delivery of peptide drugs	498:536	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	3	60	theme	drugs	532:536	arg1	bioavailability					477:491	bioavailability	477:491	bioavailability	477:491	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	3	60	theme	drugs	532:536	arg1	stability					430:438	stability	430:438	stability in the human gastrointestinal tract	430:474	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	7	61	theme	CSAD-VB12/insulin	1196:1212	arg1	nanoparticles					1214:1226	CSAD-VB12/insulin nanoparticles	1196:1226	CSAD-VB12/insulin nanoparticles	1196:1226	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells by CCK-8 test.					
31571874	7	62	theme	adenocarcinoma	1274:1287	arg1	cells					1298:1302	the human colon adenocarcinoma (Caco-2) cells	1258:1302	the human colon adenocarcinoma (Caco-2) cells	1258:1302	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells by CCK-8 test.					
31571874	14	63	theme	CSAD-VB12/insulin	2069:2085	arg1	nanoparticles					2087:2099	the CSAD-VB12/insulin nanoparticles	2065:2099	the CSAD-VB12/insulin nanoparticles	2065:2099	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice yields higher intestinal retention effect, targeted absorption, and outstanding efficacy.					
31571874	11	64	dep	RESULTS	1636:1642	arg1	They					1644:1647	They	1644:1647	They	1644:1647	RESULTS They were observed as spherical nanoparticles in the size of 30-50 nm.					
31571874	10	65	theme	test	1616:1619	arg1	nanoparticles					1621:1633	test nanoparticles	1616:1633	test nanoparticles	1616:1633	Type 1 diabetes mice were used to evaluate the intestinal absorption and retention effect of test nanoparticles.					
31571874	2	66	theme	digestive	360:368	arg1	enzymes					370:376	digestive enzymes	360:376	digestive enzymes inside the human gastrointestinal tract	360:416	However, the poor bioavailability of peptide drugs for oral administration has limited their clinical applications, on account of the acidic environment and digestive enzymes inside the human gastrointestinal tract.					
31571874	2	67	theme	clinical	296:303	arg1	applications					305:316	their clinical applications	290:316	their clinical applications	290:316	However, the poor bioavailability of peptide drugs for oral administration has limited their clinical applications, on account of the acidic environment and digestive enzymes inside the human gastrointestinal tract.					
31571874	3	68	theme	oral	546:549	arg1	administration					551:564	oral administration	546:564	oral administration	546:564	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	15	69	theme	promising	2381:2389	arg1	candidate					2391:2399	a promising candidate	2379:2399	a promising candidate for oral peptide delivery	2379:2425	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	15	69	theme	promising	2381:2389	arg1	it					2367:2368	it	2367:2368	it	2367:2368	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	9	70	theme	enterocytes	1510:1520	arg1	endocytosis					1484:1494	the endocytosis	1480:1494	the endocytosis of intestinal enterocytes	1480:1520	Furthermore, confocal was used to confirm the endocytosis of intestinal enterocytes.					
31571874	4	71	theme	1H	881:882	arg1	spectroscopy					888:899	FTIR and 1H NMR spectroscopy	872:899	spectroscopy	888:899	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	4	72	theme	vitamin	682:688	arg1	derivative					731:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	3	73	theme	amphiphilic	590:600	arg1	CSAD-VB12					630:638	CSAD-VB12	630:638	CSAD-VB12	630:638	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	3	73	theme	amphiphilic	590:600	arg1	derivative					618:627	a vitamin B12-modified amphiphilic sodium alginate derivative	567:627	a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12)	567:639	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	1	74	theme	various	185:191	arg1	diseases					193:200	therapy various diseases	177:200	therapy various diseases	177:200	PURPOSE Peptide drugs have been used in therapy various diseases.					
31571874	4	75	theme	amphiphilic	703:713	arg1	derivative					731:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	3	76	from	delivery	512:519	arg1	tract					470:474	the human gastrointestinal tract	443:474	the human gastrointestinal tract	443:474	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	3	77	theme	vitamin	569:575	arg1	CSAD-VB12					630:638	CSAD-VB12	630:638	CSAD-VB12	630:638	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	3	77	theme	vitamin	569:575	arg1	derivative					618:627	a vitamin B12-modified amphiphilic sodium alginate derivative	567:627	a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12)	567:639	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	15	78	theme	oral	2325:2328	arg1	administration					2330:2343	oral administration	2325:2343	oral administration	2325:2343	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	3	79	theme	sodium	602:607	arg1	CSAD-VB12					630:638	CSAD-VB12	630:638	CSAD-VB12	630:638	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	3	79	theme	sodium	602:607	arg1	derivative					618:627	a vitamin B12-modified amphiphilic sodium alginate derivative	567:627	a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12)	567:639	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	14	80	theme	targeted	2168:2175	arg1	absorption					2177:2186	targeted absorption	2168:2186	targeted absorption	2168:2186	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice yields higher intestinal retention effect, targeted absorption, and outstanding efficacy.					
31571874	2	81	theme	peptide	240:246	arg1	drugs					248:252	peptide drugs	240:252	peptide drugs	240:252	However, the poor bioavailability of peptide drugs for oral administration has limited their clinical applications, on account of the acidic environment and digestive enzymes inside the human gastrointestinal tract.					
31571874	8	82	theme	cell	1326:1329	arg1	model					1331:1335	Caco-2 cell model	1319:1335	Caco-2 cell model	1319:1335	Caco-2 cell model was used to measure the apparent permeability (Papp) of insulin, CSAD/insulin and CSAD-VB12/insulin.					
31571874	15	83	theme	peptide	2314:2320	arg1	absorption					2277:2286	the small intestinal absorption efficacy and retention	2256:2309	absorption	2277:2286	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	15	83	theme	peptide	2314:2320	arg1	retention					2301:2309	retention	2301:2309	retention	2301:2309	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	3	84	theme	alginate	609:616	arg1	CSAD-VB12					630:638	CSAD-VB12	630:638	CSAD-VB12	630:638	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	3	84	theme	alginate	609:616	arg1	derivative					618:627	a vitamin B12-modified amphiphilic sodium alginate derivative	567:627	a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12)	567:639	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	5	85	theme	insulin-loaded	951:964	arg1	nanoparticles					996:1008	the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles	947:1008	the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials	947:1038	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	12	86	theme	human	1811:1815	arg1	cells					1847:1851	the human colon adenocarcinoma (Caco-2) cells	1807:1851	the human colon adenocarcinoma (Caco-2) cells	1807:1851	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells.					
31571874	7	87	theme	CCK-8	1307:1311	arg1	test					1313:1316	CCK-8 test	1307:1316	CCK-8 test	1307:1316	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells by CCK-8 test.					
31571874	12	88	theme	adenocarcinoma	1823:1836	arg1	cells					1847:1851	the human colon adenocarcinoma (Caco-2) cells	1807:1851	the human colon adenocarcinoma (Caco-2) cells	1807:1851	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells.					
31571874	4	89	theme	alginate	722:729	arg1	derivative					731:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	A vitamin B12-modified amphiphilic sodium alginate derivative	680:740	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	13	90	theme	Caco-2	2023:2028	arg1	model					2035:2039	the Caco-2 cell model	2019:2039	the Caco-2 cell model	2019:2039	Comparing with insulin and the CSAD/insulin nanoparticles, the CSAD-VB12/insulin nanoparticles exhibited higher permeation ability through intestinal enterocytes in the Caco-2 cell model.					
31571874	0	91	theme	Oral	96:99	arg1	Efficacy					110:117	The Oral Delivery Efficacy	92:117	The Oral Delivery Efficacy Of Peptide Drugs	92:134	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	5	92	theme	peptide	930:936	arg1	drug					938:941	a model peptide drug	922:941	a model peptide drug	922:941	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	5	92	theme	peptide	930:936	arg1	Insulin					902:908	Insulin	902:908	Insulin	902:908	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	10	93	used	used	1549:1552	arg2	mice					1539:1542	Type 1 diabetes mice	1523:1542	Type 1 diabetes mice	1523:1542	Type 1 diabetes mice were used to evaluate the intestinal absorption and retention effect of test nanoparticles.					
31571874	10	94	theme	Type	1523:1526	arg1	diabetes					1530:1537	Type 1 diabetes	1523:1537	Type 1 diabetes mice	1523:1542	Type 1 diabetes mice were used to evaluate the intestinal absorption and retention effect of test nanoparticles.					
31571874	7	95	theme	Caco-2	1290:1295	arg1	cells					1298:1302	the human colon adenocarcinoma (Caco-2) cells	1258:1302	the human colon adenocarcinoma (Caco-2) cells	1258:1302	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells by CCK-8 test.					
31571874	3	96	from	stability	430:438	arg1	tract					470:474	the human gastrointestinal tract	443:474	the human gastrointestinal tract	443:474	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	3	97	theme	B12-modified	577:588	arg1	CSAD-VB12					630:638	CSAD-VB12	630:638	CSAD-VB12	630:638	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	3	97	theme	B12-modified	577:588	arg1	derivative					618:627	a vitamin B12-modified amphiphilic sodium alginate derivative	567:627	a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12)	567:639	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	4	98	theme	active	808:813	arg1	method					815:820	the N,N'-dicyclohexylcarbodiimide active method	774:820	method	815:820	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	13	99	theme	intestinal	1993:2002	arg1	enterocytes					2004:2014	intestinal enterocytes	1993:2014	intestinal enterocytes	1993:2014	Comparing with insulin and the CSAD/insulin nanoparticles, the CSAD-VB12/insulin nanoparticles exhibited higher permeation ability through intestinal enterocytes in the Caco-2 cell model.					
31571874	0	100	theme	Peptide	122:128	arg1	Drugs					130:134	Peptide Drugs	122:134	Peptide Drugs	122:134	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	5	101	theme	zeta	1024:1027	arg1	potentials					1029:1038	negative zeta potentials	1015:1038	negative zeta potentials	1015:1038	Insulin was used as a model peptide drug and the insulin-loaded CSAD-VB12 (CSAD-VB12/insulin) nanoparticles with negative zeta potentials were prepared in PBS (pH=7.4).					
31571874	3	102	theme	gastrointestinal	453:468	arg1	tract					470:474	the human gastrointestinal tract	443:474	the human gastrointestinal tract	443:474	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	7	103	theme	human	1262:1266	arg1	cells					1298:1302	the human colon adenocarcinoma (Caco-2) cells	1258:1302	the human colon adenocarcinoma (Caco-2) cells	1258:1302	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells by CCK-8 test.					
31571874	12	104	theme	CSAD-VB12/insulin	1745:1761	arg1	nanoparticles					1763:1775	CSAD-VB12/insulin nanoparticles	1745:1775	CSAD-VB12/insulin nanoparticles	1745:1775	The CSAD-VB12 derivatives and CSAD-VB12/insulin nanoparticles displayed nontoxicity towards the human colon adenocarcinoma (Caco-2) cells.					
31571874	10	105	theme	diabetes	1530:1537	arg1	mice					1539:1542	Type 1 diabetes mice	1523:1542	Type 1 diabetes mice	1523:1542	Type 1 diabetes mice were used to evaluate the intestinal absorption and retention effect of test nanoparticles.					
31571874	13	106	theme	higher	1959:1964	arg1	ability					1977:1983	higher permeation ability	1959:1983	higher permeation ability	1959:1983	Comparing with insulin and the CSAD/insulin nanoparticles, the CSAD-VB12/insulin nanoparticles exhibited higher permeation ability through intestinal enterocytes in the Caco-2 cell model.					
31571874	0	107	theme	B12-Modified	25:36	arg1	Derivatives					66:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	14	108	theme	intestinal	2139:2148	arg1	effect					2160:2165	higher intestinal retention effect	2132:2165	higher intestinal retention effect	2132:2165	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice yields higher intestinal retention effect, targeted absorption, and outstanding efficacy.					
31571874	4	109	theme	room	825:828	arg1	temperature					830:840	room temperature	825:840	room temperature	825:840	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	0	110	theme	Sodium	50:55	arg1	Derivatives					66:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives	17:76	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	6	111	theme	electron	1080:1087	arg1	microscopy					1089:1098	Scanning electron microscopy	1071:1098	Scanning electron microscopy	1071:1098	Scanning electron microscopy was used to observe CSAD-VB12/insulin as spherical nanoparticles.					
31571874	0	112	theme	Derivatives	66:76	arg1	Investigation					0:12	Investigation	0:12	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.	0:135	Investigation Of Vitamin B12-Modified Amphiphilic Sodium Alginate Derivatives For Enhancing The Oral Delivery Efficacy Of Peptide Drugs.					
31571874	13	113	theme	CSAD-VB12/insulin	1917:1933	arg1	nanoparticles					1935:1947	the CSAD-VB12/insulin nanoparticles	1913:1947	the CSAD-VB12/insulin nanoparticles	1913:1947	Comparing with insulin and the CSAD/insulin nanoparticles, the CSAD-VB12/insulin nanoparticles exhibited higher permeation ability through intestinal enterocytes in the Caco-2 cell model.					
31571874	14	114	theme	diabetic	2111:2118	arg1	mice					2120:2123	Type 1 diabetic mice	2104:2123	Type 1 diabetic mice	2104:2123	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice yields higher intestinal retention effect, targeted absorption, and outstanding efficacy.					
31571874	10	115	theme	retention	1596:1604	arg1	effect					1606:1611	the intestinal absorption and retention effect	1566:1611	the intestinal absorption and retention effect of test nanoparticles	1566:1633	Type 1 diabetes mice were used to evaluate the intestinal absorption and retention effect of test nanoparticles.					
31571874	4	116	theme	FTIR	872:875	arg1	spectroscopy					888:899	FTIR and 1H NMR spectroscopy	872:899	spectroscopy	888:899	MATERIALS AND METHODS A vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized via the N,N'-dicyclohexylcarbodiimide active method at room temperature, and then characterized using FTIR and 1H NMR spectroscopy.					
31571874	3	117	from	bioavailability	477:491	arg1	tract					470:474	the human gastrointestinal tract	443:474	the human gastrointestinal tract	443:474	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	2	118	theme	acidic	337:342	arg1	environment					344:354	the acidic environment	333:354	the acidic environment	333:354	However, the poor bioavailability of peptide drugs for oral administration has limited their clinical applications, on account of the acidic environment and digestive enzymes inside the human gastrointestinal tract.					
31571874	15	119	theme	small	2260:2264	arg1	absorption					2277:2286	the small intestinal absorption efficacy and retention	2256:2309	absorption	2277:2286	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	15	120	theme	intestinal	2266:2275	arg1	absorption					2277:2286	the small intestinal absorption efficacy and retention	2256:2309	absorption	2277:2286	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	10	121	theme	absorption	1581:1590	arg1	effect					1606:1611	the intestinal absorption and retention effect	1566:1611	the intestinal absorption and retention effect of test nanoparticles	1566:1633	Type 1 diabetes mice were used to evaluate the intestinal absorption and retention effect of test nanoparticles.					
31571874	3	122	theme	targeted	498:505	arg1	delivery					512:519	targeted drug delivery	498:519	targeted drug delivery of peptide drugs	498:536	To enhance stability in the human gastrointestinal tract, bioavailability, and targeted drug delivery of peptide drugs through oral administration, a vitamin B12-modified amphiphilic sodium alginate derivative (CSAD-VB12) was synthesized.					
31571874	15	123	theme	CONCLUSION	2215:2224	arg1	derivatives					2236:2246	CONCLUSION CSAD-VB12 derivatives	2215:2246	CONCLUSION CSAD-VB12 derivatives	2215:2246	CONCLUSION CSAD-VB12 derivatives enhance the small intestinal absorption efficacy and retention of peptide by oral administration, which indicated that it could be a promising candidate for oral peptide delivery in the prospective clinical application.					
31571874	14	124	theme	nanoparticles	2087:2099	arg1	administration					2047:2060	Oral administration	2042:2060	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice	2042:2123	Oral administration of the CSAD-VB12/insulin nanoparticles to Type 1 diabetic mice yields higher intestinal retention effect, targeted absorption, and outstanding efficacy.					
31571874	2	125	theme	gastrointestinal	395:410	arg1	tract					412:416	the human gastrointestinal tract	385:416	the human gastrointestinal tract	385:416	However, the poor bioavailability of peptide drugs for oral administration has limited their clinical applications, on account of the acidic environment and digestive enzymes inside the human gastrointestinal tract.					
31571874	10	126	theme	nanoparticles	1621:1633	arg1	effect					1606:1611	the intestinal absorption and retention effect	1566:1611	the intestinal absorption and retention effect of test nanoparticles	1566:1633	Type 1 diabetes mice were used to evaluate the intestinal absorption and retention effect of test nanoparticles.					
30867067	13	0	theme	Clostridium	2391:2401	arg1	Ruminococcaceae					2374:2388	Ruminococcaceae	2374:2388	Ruminococcaceae	2374:2388	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	13	0	theme	Clostridium	2391:2401	arg1	cluster					2403:2409	Clostridium cluster IV	2391:2412	Clostridium cluster IV	2391:2412	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	8	1	theme	human	1553:1557	arg1	donor					1559:1563	the respective healthy or frail human donor	1521:1563	donor	1559:1563	RESULTS Humanized mice maintained gut microbiota types reflecting the respective healthy or frail human donor.					
30867067	13	2	theme	chemokine	2485:2493	arg1	CXCL11					2495:2500	the chemokine CXCL11	2481:2500	the chemokine CXCL11	2481:2500	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	1	3	theme	microbiota	229:238	arg1	modulation					207:216	modulation	207:216	modulation of the gut microbiota by diet	207:246	BACKGROUND There are complex interactions between aging, frailty, diet, and the gut microbiota; modulation of the gut microbiota by diet could lead to healthier aging.					
30867067	7	4	from	cytokines	1434:1442	arg1	mice					1418:1421	mice	1418:1421	mice	1418:1421	The gut microbiota was profiled and immune responses were assayed by T cell markers in mice, and serum cytokines in humans.					
30867067	7	4	from	cytokines	1434:1442	arg1	humans					1447:1452	humans	1447:1452	humans	1447:1452	The gut microbiota was profiled and immune responses were assayed by T cell markers in mice, and serum cytokines in humans.					
30867067	7	5	theme	cell	1402:1405	arg1	markers					1407:1413	T cell markers	1400:1413	T cell markers in mice	1400:1421	The gut microbiota was profiled and immune responses were assayed by T cell markers in mice, and serum cytokines in humans.					
30867067	14	6	theme	elderly	2813:2819	arg1	individuals					2841:2851	elderly frail and non-frail individuals	2813:2851	elderly frail and non-frail individuals	2813:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	14	7	from	changes	2712:2718	arg1	microbiota					2731:2740	the gut microbiota	2723:2740	the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals	2723:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	14	7	from	changes	2712:2718	arg1	donors					2755:2760	healthy donors	2747:2760	healthy donors reflecting previously observed differences between elderly frail and non-frail individuals	2747:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	6	8	theme	combined	1212:1219	arg1	total					1221:1225	10 g/day combined total	1203:1225	10 g/day combined total	1203:1225	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	12	9	theme	innate	1958:1963	arg1	markers					1972:1978	innate immune markers	1958:1978	innate immune markers in both conventional and germ-free mice	1958:2018	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	9	10	theme	specific	1590:1597	arg1	taxa					1599:1602	specific taxa	1590:1602	specific taxa	1590:1602	Changes in abundance of specific taxa occurred with the diet switch.					
30867067	6	11	dep	prebiotics	1092:1101	arg1	galactooligo-saccharide					1175:1197	galactooligo-saccharide	1175:1197	galactooligo-saccharide	1175:1197	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	11	dep	prebiotics	1092:1101	arg1	dextrin					1110:1116	wheat dextrin	1104:1116	wheat dextrin	1104:1116	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	11	dep	prebiotics	1092:1101	arg1	starch					1129:1134	resistant starch	1119:1134	resistant starch	1119:1134	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	11	dep	prebiotics	1092:1101	arg1	fiber					1164:1168	soluble corn fiber	1151:1168	soluble corn fiber	1151:1168	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	11	dep	prebiotics	1092:1101	arg1	prebiotics					1092:1101	five prebiotics	1087:1101	five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide)	1087:1198	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	11	dep	prebiotics	1092:1101	arg1	polydextrose					1137:1148	polydextrose	1137:1148	polydextrose	1137:1148	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	14	12	from	donors	2755:2760	arg1	changes					2712:2718	changes	2712:2718	changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals	2712:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	14	12	from	donors	2755:2760	arg1	microbiota					2731:2740	the gut microbiota	2723:2740	the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals	2723:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	10	13	theme	community	1652:1660	arg1	microbiota					1667:1676	the community type microbiota	1648:1676	the community type microbiota	1648:1676	In mice with the community type microbiota, the observed differences reflected compositions previously associated with higher frailty.					
30867067	4	14	theme	residential	822:832	arg1	care					834:837	long-stay residential care	812:837	long-stay residential care	812:837	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	7	15	theme	gut	1335:1337	arg1	microbiota					1339:1348	The gut microbiota	1331:1348	The gut microbiota	1331:1348	The gut microbiota was profiled and immune responses were assayed by T cell markers in mice, and serum cytokines in humans.					
30867067	12	16	from	markers	1972:1978	arg1	mice					2015:2018	both conventional and germ-free mice	1983:2018	both conventional and germ-free mice	1983:2018	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	3	17	theme	6-month	494:500	arg1	supplementation					516:530	a 6-month dietary fiber supplementation	492:530	a 6-month dietary fiber supplementation	492:530	We also performed a 6-month dietary fiber supplementation in three human cohorts representing three distinct life-stages.					
30867067	15	18	from	effects	3041:3047	arg1	humans					3052:3057	humans	3052:3057	humans	3052:3057	However, the frailty-associated gut microbiota did not reciprocally switch to a younger healthy-subject like state, and supplementation with prebiotics was associated with fewer detected effects in humans than diet adjustment in animal models.					
30867067	6	19	theme	prebiotics	1092:1101	arg1	mix					1080:1082	a mix	1078:1082	a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total	1078:1225	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	12	20	theme	Blautia	2141:2147	arg1	abundance					2111:2119	abundance	2111:2119	abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium	2111:2198	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	6	21	with	20 g/day	1253:1260	arg1	mix					1080:1082	a mix	1078:1082	a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total	1078:1225	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	12	22	theme	changes	2056:2062	arg1	number					2036:2041	a moderate number	2025:2041	a moderate number of taxonomic changes occurring upon diet	2025:2082	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	7	23	from	markers	1407:1413	arg1	mice					1418:1421	mice	1418:1421	mice	1418:1421	The gut microbiota was profiled and immune responses were assayed by T cell markers in mice, and serum cytokines in humans.					
30867067	7	23	from	markers	1407:1413	arg1	humans					1447:1452	humans	1447:1452	humans	1447:1452	The gut microbiota was profiled and immune responses were assayed by T cell markers in mice, and serum cytokines in humans.					
30867067	15	24	theme	diet	3064:3067	arg1	adjustment					3069:3078	diet adjustment	3064:3078	diet adjustment	3064:3078	However, the frailty-associated gut microbiota did not reciprocally switch to a younger healthy-subject like state, and supplementation with prebiotics was associated with fewer detected effects in humans than diet adjustment in animal models.					
30867067	14	25	theme	gut	2727:2729	arg1	microbiota					2731:2740	the gut microbiota	2723:2740	the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals	2723:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	4	26	with	intervention	683:694	arg1	diet					729:732	a high-fiber/low-fat diet	708:732	a high-fiber/low-fat diet typical of elderly community dwellers	708:770	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	4	26	with	intervention	683:694	arg1	diet					796:799	a low-fiber/high-fat diet	775:799	a low-fiber/high-fat diet	775:799	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	14	27	theme	non-frail	2831:2839	arg1	individuals					2841:2851	elderly frail and non-frail individuals	2813:2851	elderly frail and non-frail individuals	2813:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	3	28	theme	fiber	510:514	arg1	supplementation					516:530	a 6-month dietary fiber supplementation	492:530	a 6-month dietary fiber supplementation	492:530	We also performed a 6-month dietary fiber supplementation in three human cohorts representing three distinct life-stages.					
30867067	6	29	theme	soluble	1151:1157	arg1	fiber					1164:1168	soluble corn fiber	1151:1168	soluble corn fiber	1151:1168	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	29	theme	soluble	1151:1157	arg1	prebiotics					1092:1101	five prebiotics	1087:1101	five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide)	1087:1198	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	4	30	theme	8-week	668:673	arg1	intervention					683:694	an 8-week dietary intervention	665:694	an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects	665:846	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	6	31	theme	healthy	1232:1238	arg1	subjects					1240:1247	healthy subjects	1232:1247	healthy subjects	1232:1247	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	32	with	placebo	1285:1291	arg1	mix					1080:1082	a mix	1078:1082	a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total	1078:1225	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	1	33	theme	complex	132:138	arg1	interactions					140:151	complex interactions	132:151	complex interactions between aging, frailty, diet, and the gut microbiota	132:204	BACKGROUND There are complex interactions between aging, frailty, diet, and the gut microbiota; modulation of the gut microbiota by diet could lead to healthier aging.					
30867067	12	34	theme	moderate	2027:2034	arg1	number					2036:2041	a moderate number	2025:2041	a moderate number of taxonomic changes occurring upon diet	2025:2082	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	15	35	theme	frailty-associated	2867:2884	arg1	microbiota					2890:2899	the frailty-associated gut microbiota	2863:2899	the frailty-associated gut microbiota	2863:2899	However, the frailty-associated gut microbiota did not reciprocally switch to a younger healthy-subject like state, and supplementation with prebiotics was associated with fewer detected effects in humans than diet adjustment in animal models.					
30867067	4	36	theme	human	629:633	arg1	microbiota					635:644	human microbiota	629:644	human microbiota	629:644	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	0	37	theme	gut	65:67	arg1	taxa					80:83	specific gut microbiota taxa	56:83	specific gut microbiota taxa but not global diversity	56:108	Prebiotic supplementation in frail older people affects specific gut microbiota taxa but not global diversity.					
30867067	3	38	theme	human	541:545	arg1	cohorts					547:553	three human cohorts	535:553	three human cohorts representing three distinct life-stages	535:593	We also performed a 6-month dietary fiber supplementation in three human cohorts representing three distinct life-stages.					
30867067	4	39	dep	diet	729:732	arg1	subjects					839:846	typical of long-stay residential care subjects	801:846	typical of long-stay residential care subjects	801:846	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	12	40	theme	differential	1935:1946	arg1	effect					1948:1953	a differential effect	1933:1953	a differential effect on innate immune markers in both conventional and germ-free mice	1933:2018	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	14	41	theme	frail	2821:2825	arg1	individuals					2841:2851	elderly frail and non-frail individuals	2813:2851	elderly frail and non-frail individuals	2813:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	13	42	theme	human	2208:2212	arg1	intervention					2214:2225	the human intervention	2204:2225	the human intervention	2204:2225	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	2	43	theme	frail	454:458	arg1	people					466:471	healthy or frail older people	443:471	healthy or frail older people	443:471	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	4	44	theme	high-fiber/low-fat	710:727	arg1	diet					729:732	a high-fiber/low-fat diet	708:732	a high-fiber/low-fat diet typical of elderly community dwellers	708:770	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	6	45	theme	resistant	1119:1127	arg1	prebiotics					1092:1101	five prebiotics	1087:1101	five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide)	1087:1198	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	45	theme	resistant	1119:1127	arg1	starch					1129:1134	resistant starch	1119:1134	resistant starch	1119:1134	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	5	46	theme	other	929:933	arg1	type					940:943	the other diet type	925:943	the other diet type	925:943	A cross-over design was used where the diets were switched after 4 weeks to the other diet type to identify responsive taxa and innate immunity changes.					
30867067	2	47	theme	healthy	443:449	arg1	frail					454:458	healthy or frail	443:458	healthy or frail older people	443:471	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	1	48	dep	BACKGROUND	111:120	arg1	are					128:130	are	128:130	are complex interactions between aging, frailty, diet, and the gut microbiota	128:204	BACKGROUND There are complex interactions between aging, frailty, diet, and the gut microbiota; modulation of the gut microbiota by diet could lead to healthier aging.					
30867067	14	49	from	microbiota	2679:2688	arg1	models					2699:2704	mouse models	2693:2704	mouse models	2693:2704	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	14	50	theme	mouse	2693:2697	arg1	models					2699:2704	mouse models	2693:2704	mouse models	2693:2704	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	13	51	theme	wash-out	2580:2587	arg1	period					2589:2594	the wash-out period	2576:2594	the wash-out period	2576:2594	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	2	52	theme	diets	331:335	arg1	effect					321:326	the effect	317:326	the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people	317:471	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	0	53	theme	Prebiotic	0:8	arg1	supplementation					10:24	Prebiotic supplementation	0:24	Prebiotic supplementation in frail older people	0:46	Prebiotic supplementation in frail older people affects specific gut microbiota taxa but not global diversity.					
30867067	15	54	with	supplementation	2974:2988	arg1	prebiotics					2995:3004	prebiotics	2995:3004	prebiotics	2995:3004	However, the frailty-associated gut microbiota did not reciprocally switch to a younger healthy-subject like state, and supplementation with prebiotics was associated with fewer detected effects in humans than diet adjustment in animal models.					
30867067	11	55	attach	present	1798:1804	arg1	mice					1840:1843	community inoculated mice	1819:1843	community inoculated mice	1819:1843	The dominance of Prevotella present initially in community inoculated mice was replaced by Bacteroides, Alistipes, and Oscillibacter.					
30867067	11	55	attach	present	1798:1804	arg2	Prevotella					1787:1796	Prevotella	1787:1796	Prevotella present initially in community inoculated mice	1787:1843	The dominance of Prevotella present initially in community inoculated mice was replaced by Bacteroides, Alistipes, and Oscillibacter.					
30867067	2	56	theme	fiber	366:370	arg1	content					372:378	fiber content	366:378	fiber content	366:378	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	11	57	from	present	1798:1804	arg1	mice					1840:1843	community inoculated mice	1819:1843	community inoculated mice	1819:1843	The dominance of Prevotella present initially in community inoculated mice was replaced by Bacteroides, Alistipes, and Oscillibacter.					
30867067	12	58	theme	cluster	2162:2168	arg1	abundance					2111:2119	abundance	2111:2119	abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium	2111:2198	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	12	59	theme	Phascolarctobacterium	2178:2198	arg1	abundance					2111:2119	abundance	2111:2119	abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium	2111:2198	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	0	60	theme	older	35:39	arg1	people					41:46	frail older people	29:46	frail older people	29:46	Prebiotic supplementation in frail older people affects specific gut microbiota taxa but not global diversity.					
30867067	5	61	theme	innate	977:982	arg1	immunity					984:991	innate immunity	977:991	innate immunity	977:991	A cross-over design was used where the diets were switched after 4 weeks to the other diet type to identify responsive taxa and innate immunity changes.					
30867067	14	62	theme	profound	2656:2663	arg1	effect					2665:2670	a profound effect	2654:2670	a profound effect	2654:2670	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	2	63	theme	gut	389:391	arg1	microbiota					393:402	the gut microbiota	385:402	the gut microbiota of mice humanized with microbiota from healthy or frail older people	385:471	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	8	64	theme	gut	1489:1491	arg1	types					1504:1508	gut microbiota types	1489:1508	gut microbiota types reflecting the respective healthy or frail human donor	1489:1563	RESULTS Humanized mice maintained gut microbiota types reflecting the respective healthy or frail human donor.					
30867067	3	65	theme	distinct	574:581	arg1	life-stages					583:593	three distinct life-stages	568:593	three distinct life-stages	568:593	We also performed a 6-month dietary fiber supplementation in three human cohorts representing three distinct life-stages.					
30867067	12	66	from	effect	1948:1953	arg1	markers					1972:1978	innate immune markers	1958:1978	innate immune markers in both conventional and germ-free mice	1958:2018	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	11	67	theme	Prevotella	1787:1796	arg1	dominance					1774:1782	The dominance	1770:1782	The dominance of Prevotella present initially in community inoculated mice	1770:1843	The dominance of Prevotella present initially in community inoculated mice was replaced by Bacteroides, Alistipes, and Oscillibacter.					
30867067	10	68	with	mice	1638:1641	arg1	microbiota					1667:1676	the community type microbiota	1648:1676	the community type microbiota	1648:1676	In mice with the community type microbiota, the observed differences reflected compositions previously associated with higher frailty.					
30867067	9	69	theme	taxa	1599:1602	arg1	abundance					1577:1585	abundance	1577:1585	abundance of specific taxa	1577:1602	Changes in abundance of specific taxa occurred with the diet switch.					
30867067	10	70	theme	higher	1754:1759	arg1	frailty					1761:1767	higher frailty	1754:1767	higher frailty	1754:1767	In mice with the community type microbiota, the observed differences reflected compositions previously associated with higher frailty.					
30867067	4	71	theme	elderly	745:751	arg1	dwellers					763:770	elderly community dwellers	745:770	elderly community dwellers	745:770	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	6	72	from	total	1221:1225	arg1	mix					1080:1082	a mix	1078:1082	a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total	1078:1225	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	14	73	theme	poorer	2638:2643	arg1	diet					2645:2648	a nutritionally poorer diet	2622:2648	a nutritionally poorer diet	2622:2648	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	15	74	theme	younger	2934:2940	arg1	healthy-subject					2942:2956	a younger healthy-subject	2932:2956	a younger healthy-subject	2932:2956	However, the frailty-associated gut microbiota did not reciprocally switch to a younger healthy-subject like state, and supplementation with prebiotics was associated with fewer detected effects in humans than diet adjustment in animal models.					
30867067	4	75	theme	dwellers	763:770	arg1	typical					734:740	typical	734:740	typical	734:740	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	11	76	theme	community	1819:1827	arg1	mice					1840:1843	community inoculated mice	1819:1843	community inoculated mice	1819:1843	The dominance of Prevotella present initially in community inoculated mice was replaced by Bacteroides, Alistipes, and Oscillibacter.					
30867067	12	77	theme	immune	1965:1970	arg1	markers					1972:1978	innate immune markers	1958:1978	innate immune markers in both conventional and germ-free mice	1958:2018	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	5	78	theme	taxa	968:971	arg1	changes					993:999	responsive taxa and innate immunity changes	957:999	responsive taxa and innate immunity changes	957:999	A cross-over design was used where the diets were switched after 4 weeks to the other diet type to identify responsive taxa and innate immunity changes.					
30867067	6	79	theme	normal	1061:1066	arg1	diet					1068:1071	their normal diet	1055:1071	their normal diet	1055:1071	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	13	80	from	changes	2279:2285	arg1	beta-diversity					2300:2313	beta-diversity	2300:2313	beta-diversity	2300:2313	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	13	80	from	changes	2279:2285	arg1	alpha-					2290:2295	alpha-	2290:2295	alpha-	2290:2295	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	12	81	from	increase	2099:2106	arg1	abundance					2111:2119	abundance	2111:2119	abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium	2111:2198	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	1	82	theme	gut	225:227	arg1	microbiota					229:238	the gut microbiota	221:238	the gut microbiota	221:238	BACKGROUND There are complex interactions between aging, frailty, diet, and the gut microbiota; modulation of the gut microbiota by diet could lead to healthier aging.					
30867067	7	83	theme	T	1400:1400	arg1	markers					1407:1413	T cell markers	1400:1413	T cell markers in mice	1400:1421	The gut microbiota was profiled and immune responses were assayed by T cell markers in mice, and serum cytokines in humans.					
30867067	4	84	theme	care	834:837	arg1	typical					801:807	typical	801:807	typical	801:807	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	8	85	theme	frail	1547:1551	arg1	donor					1559:1563	the respective healthy or frail human donor	1521:1563	donor	1559:1563	RESULTS Humanized mice maintained gut microbiota types reflecting the respective healthy or frail human donor.					
30867067	13	86	theme	CXCL11	2495:2500	arg1	levels					2471:2476	levels	2471:2476	levels of the chemokine CXCL11	2471:2500	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	6	87	theme	wheat	1104:1108	arg1	prebiotics					1092:1101	five prebiotics	1087:1101	five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide)	1087:1198	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	87	theme	wheat	1104:1108	arg1	dextrin					1110:1116	wheat dextrin	1104:1116	wheat dextrin	1104:1116	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	7	88	theme	immune	1367:1372	arg1	responses					1374:1382	immune responses	1367:1382	immune responses	1367:1382	The gut microbiota was profiled and immune responses were assayed by T cell markers in mice, and serum cytokines in humans.					
30867067	6	89	theme	10 g/day	1203:1210	arg1	total					1221:1225	10 g/day combined total	1203:1225	10 g/day combined total	1203:1225	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	10	90	theme	type	1662:1665	arg1	microbiota					1667:1676	the community type microbiota	1648:1676	the community type microbiota	1648:1676	In mice with the community type microbiota, the observed differences reflected compositions previously associated with higher frailty.					
30867067	13	91	theme	global	2272:2277	arg1	changes					2279:2285	any global changes	2268:2285	any global changes in alpha- or beta-diversity	2268:2313	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	6	92	with	diet	1068:1071	arg1	mix					1080:1082	a mix	1078:1082	a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total	1078:1225	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	4	93	theme	typical	801:807	arg1	subjects					839:846	typical of long-stay residential care subjects	801:846	typical of long-stay residential care subjects	801:846	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	14	94	theme	observed	2784:2791	arg1	differences					2793:2803	previously observed differences	2773:2803	previously observed differences between elderly frail and non-frail individuals	2773:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	4	95	theme	METHODS	596:602	arg1	Mice					604:607	METHODS Mice	596:607	METHODS Mice	596:607	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	15	96	theme	animal	3083:3088	arg1	models					3090:3095	animal models	3083:3095	animal models	3083:3095	However, the frailty-associated gut microbiota did not reciprocally switch to a younger healthy-subject like state, and supplementation with prebiotics was associated with fewer detected effects in humans than diet adjustment in animal models.					
30867067	12	97	theme	type	1910:1913	arg1	microbiota					1915:1924	Frail type microbiota	1904:1924	Frail type microbiota	1904:1924	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	14	98	theme	CONCLUSIONS	2597:2607	arg1	Switching					2609:2617	CONCLUSIONS Switching	2597:2617	CONCLUSIONS Switching to a nutritionally poorer diet	2597:2648	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	4	99	theme	long-stay	812:820	arg1	care					834:837	long-stay residential care	812:837	long-stay residential care	812:837	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	13	100	theme	certain	2337:2343	arg1	taxa					2355:2358	certain bacterial taxa	2337:2358	certain bacterial taxa	2337:2358	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	13	100	theme	certain	2337:2343	arg1	Ruminococcaceae					2374:2388	Ruminococcaceae	2374:2388	Ruminococcaceae	2374:2388	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	10	101	theme	observed	1683:1690	arg1	differences					1692:1702	the observed differences	1679:1702	the observed differences	1679:1702	In mice with the community type microbiota, the observed differences reflected compositions previously associated with higher frailty.					
30867067	9	102	theme	diet	1622:1625	arg1	switch					1627:1632	the diet switch	1618:1632	the diet switch	1618:1632	Changes in abundance of specific taxa occurred with the diet switch.					
30867067	14	103	theme	healthy	2747:2753	arg1	donors					2755:2760	healthy donors	2747:2760	healthy donors reflecting previously observed differences between elderly frail and non-frail individuals	2747:2851	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	11	104	theme	present	1798:1804	arg1	Prevotella					1787:1796	Prevotella	1787:1796	Prevotella present initially in community inoculated mice	1787:1843	The dominance of Prevotella present initially in community inoculated mice was replaced by Bacteroides, Alistipes, and Oscillibacter.					
30867067	6	105	theme	frail	1266:1270	arg1	subjects					1272:1279	frail subjects	1266:1279	frail subjects	1266:1279	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	13	106	theme	elderly	2540:2546	arg1	group					2548:2552	the frail elderly group	2530:2552	the frail elderly group	2530:2552	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	5	107	used	used	873:876	arg2	design					862:867	A cross-over design	849:867	A cross-over design	849:867	A cross-over design was used where the diets were switched after 4 weeks to the other diet type to identify responsive taxa and innate immunity changes.					
30867067	1	108	theme	gut	191:193	arg1	microbiota					195:204	the gut microbiota	187:204	the gut microbiota	187:204	BACKGROUND There are complex interactions between aging, frailty, diet, and the gut microbiota; modulation of the gut microbiota by diet could lead to healthier aging.					
30867067	2	109	theme	older	460:464	arg1	people					466:471	healthy or frail older people	443:471	healthy or frail older people	443:471	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	12	110	theme	taxonomic	2046:2054	arg1	changes					2056:2062	taxonomic changes	2046:2062	taxonomic changes occurring upon diet	2046:2082	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	6	111	theme	10 g/day	1294:1301	arg1	placebo					1285:1291	placebo	1285:1291	placebo (10 g/day maltodextrin)	1285:1315	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	111	theme	10 g/day	1294:1301	arg1	maltodextrin					1303:1314	10 g/day maltodextrin	1294:1314	10 g/day maltodextrin	1294:1314	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	3	112	theme	dietary	502:508	arg1	supplementation					516:530	a 6-month dietary fiber supplementation	492:530	a 6-month dietary fiber supplementation	492:530	We also performed a 6-month dietary fiber supplementation in three human cohorts representing three distinct life-stages.					
30867067	9	113	from	Changes	1566:1572	arg1	abundance					1577:1585	abundance	1577:1585	abundance of specific taxa	1577:1602	Changes in abundance of specific taxa occurred with the diet switch.					
30867067	6	114	theme	corn	1159:1162	arg1	fiber					1164:1168	soluble corn fiber	1151:1168	soluble corn fiber	1151:1168	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	6	114	theme	corn	1159:1162	arg1	prebiotics					1092:1101	five prebiotics	1087:1101	five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide)	1087:1198	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	13	115	theme	prebiotic	2228:2236	arg1	supplementation					2238:2252	prebiotic supplementation	2228:2252	prebiotic supplementation	2228:2252	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	11	116	from	mice	1840:1843	arg1	present					1798:1804	present	1798:1804	present	1798:1804	The dominance of Prevotella present initially in community inoculated mice was replaced by Bacteroides, Alistipes, and Oscillibacter.					
30867067	0	117	theme	microbiota	69:78	arg1	taxa					80:83	specific gut microbiota taxa	56:83	specific gut microbiota taxa but not global diversity	56:108	Prebiotic supplementation in frail older people affects specific gut microbiota taxa but not global diversity.					
30867067	8	118	dep	RESULTS	1455:1461	arg1	maintained					1478:1487	maintained	1478:1487	maintained gut microbiota types reflecting the respective healthy or frail human donor	1478:1563	RESULTS Humanized mice maintained gut microbiota types reflecting the respective healthy or frail human donor.					
30867067	5	119	theme	cross-over	851:860	arg1	design					862:867	A cross-over design	849:867	A cross-over design	849:867	A cross-over design was used where the diets were switched after 4 weeks to the other diet type to identify responsive taxa and innate immunity changes.					
30867067	15	120	theme	gut	2886:2888	arg1	microbiota					2890:2899	the frailty-associated gut microbiota	2863:2899	the frailty-associated gut microbiota	2863:2899	However, the frailty-associated gut microbiota did not reciprocally switch to a younger healthy-subject like state, and supplementation with prebiotics was associated with fewer detected effects in humans than diet adjustment in animal models.					
30867067	12	121	theme	conventional	1988:1999	arg1	mice					2015:2018	both conventional and germ-free mice	1983:2018	both conventional and germ-free mice	1983:2018	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	2	122	theme	mice	407:410	arg1	microbiota					393:402	the gut microbiota	385:402	the gut microbiota of mice humanized with microbiota from healthy or frail older people	385:471	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	0	123	from	supplementation	10:24	arg1	people					41:46	frail older people	29:46	frail older people	29:46	Prebiotic supplementation in frail older people affects specific gut microbiota taxa but not global diversity.					
30867067	12	124	theme	germ-free	2005:2013	arg1	mice					2015:2018	both conventional and germ-free mice	1983:2018	both conventional and germ-free mice	1983:2018	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	0	125	theme	global	93:98	arg1	diversity					100:108	global diversity	93:108	specific gut microbiota taxa but not global diversity	56:108	Prebiotic supplementation in frail older people affects specific gut microbiota taxa but not global diversity.					
30867067	2	126	theme	or	451:452	arg1	frail					454:458	healthy or frail	443:458	healthy or frail older people	443:471	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	5	127	theme	diet	935:938	arg1	type					940:943	the other diet type	925:943	the other diet type	925:943	A cross-over design was used where the diets were switched after 4 weeks to the other diet type to identify responsive taxa and innate immunity changes.					
30867067	13	128	theme	frail	2534:2538	arg1	group					2548:2552	the frail elderly group	2530:2552	the frail elderly group	2530:2552	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	13	129	theme	bacterial	2345:2353	arg1	taxa					2355:2358	certain bacterial taxa	2337:2358	certain bacterial taxa	2337:2358	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	13	129	theme	bacterial	2345:2353	arg1	Ruminococcaceae					2374:2388	Ruminococcaceae	2374:2388	Ruminococcaceae	2374:2388	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	8	130	theme	Humanized	1463:1471	arg1	mice					1473:1476	Humanized mice	1463:1476	Humanized mice	1463:1476	RESULTS Humanized mice maintained gut microbiota types reflecting the respective healthy or frail human donor.					
30867067	4	131	theme	dietary	675:681	arg1	intervention					683:694	an 8-week dietary intervention	665:694	an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects	665:846	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	0	132	theme	frail	29:33	arg1	people					41:46	frail older people	29:46	frail older people	29:46	Prebiotic supplementation in frail older people affects specific gut microbiota taxa but not global diversity.					
30867067	6	133	theme	human	1009:1013	arg1	intervention					1015:1026	the human intervention	1005:1026	the human intervention	1005:1026	In the human intervention, the subjects supplemented their normal diet with a mix of five prebiotics (wheat dextrin, resistant starch, polydextrose, soluble corn fiber, and galactooligo-saccharide) at 10 g/day combined total, for healthy subjects and 20 g/day for frail subjects, or placebo (10 g/day maltodextrin) for 26 weeks.					
30867067	14	134	contain	has	2650:2652	arg1	Switching					2609:2617	CONCLUSIONS Switching	2597:2617	CONCLUSIONS Switching to a nutritionally poorer diet	2597:2648	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	14	134	contain	has	2650:2652	arg2	effect					2665:2670	a profound effect	2654:2670	a profound effect	2654:2670	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	4	135	theme	typical	734:740	arg1	diet					729:732	a high-fiber/low-fat diet	708:732	a high-fiber/low-fat diet typical of elderly community dwellers	708:770	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	12	136	theme	Clostridium	2150:2160	arg1	cluster					2162:2168	Clostridium cluster IV	2150:2171	Clostridium cluster IV	2150:2171	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	1	137	theme	healthier	262:270	arg1	aging					272:276	healthier aging	262:276	healthier aging	262:276	BACKGROUND There are complex interactions between aging, frailty, diet, and the gut microbiota; modulation of the gut microbiota by diet could lead to healthier aging.					
30867067	8	138	theme	microbiota	1493:1502	arg1	types					1504:1508	gut microbiota types	1489:1508	gut microbiota types reflecting the respective healthy or frail human donor	1489:1563	RESULTS Humanized mice maintained gut microbiota types reflecting the respective healthy or frail human donor.					
30867067	13	139	theme	taxa	2355:2358	arg1	abundance					2324:2332	the abundance	2320:2332	the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium,	2320:2454	In the human intervention, prebiotic supplementation did not drive any global changes in alpha- or beta-diversity, but the abundance of certain bacterial taxa, particularly Ruminococcaceae (Clostridium cluster IV), Parabacteroides, Phascolarctobacterium, increased, and levels of the chemokine CXCL11 were significantly lower in the frail elderly group, but increased during the wash-out period.					
30867067	0	140	theme	specific	56:63	arg1	taxa					80:83	specific gut microbiota taxa	56:83	specific gut microbiota taxa but not global diversity	56:108	Prebiotic supplementation in frail older people affects specific gut microbiota taxa but not global diversity.					
30867067	5	141	theme	immunity	984:991	arg1	changes					993:999	responsive taxa and innate immunity changes	957:999	responsive taxa and innate immunity changes	957:999	A cross-over design was used where the diets were switched after 4 weeks to the other diet type to identify responsive taxa and innate immunity changes.					
30867067	4	142	theme	low-fiber/high-fat	777:794	arg1	diet					796:799	a low-fiber/high-fat diet	775:799	a low-fiber/high-fat diet	775:799	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	2	143	theme	study	299:303	arg1	purpose					283:289	The purpose	279:289	The purpose of this study	279:303	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	7	144	theme	serum	1428:1432	arg1	cytokines					1434:1442	serum cytokines	1428:1442	serum cytokines in humans	1428:1452	The gut microbiota was profiled and immune responses were assayed by T cell markers in mice, and serum cytokines in humans.					
30867067	2	145	from	people	466:471	arg1	microbiota					427:436	microbiota	427:436	microbiota from healthy or frail older people	427:471	The purpose of this study was to test the effect of diets differing in sugar, fat, and fiber content upon the gut microbiota of mice humanized with microbiota from healthy or frail older people.					
30867067	5	146	theme	responsive	957:966	arg1	taxa					968:971	responsive taxa	957:971	responsive taxa	957:971	A cross-over design was used where the diets were switched after 4 weeks to the other diet type to identify responsive taxa and innate immunity changes.					
30867067	14	147	theme	nutritionally	2624:2636	arg1	diet					2645:2648	a nutritionally poorer diet	2622:2648	a nutritionally poorer diet	2622:2648	CONCLUSIONS Switching to a nutritionally poorer diet has a profound effect on the microbiota in mouse models, with changes in the gut microbiota from healthy donors reflecting previously observed differences between elderly frail and non-frail individuals.					
30867067	12	148	theme	Frail	1904:1908	arg1	microbiota					1915:1924	Frail type microbiota	1904:1924	Frail type microbiota	1904:1924	Frail type microbiota showed a differential effect on innate immune markers in both conventional and germ-free mice, but a moderate number of taxonomic changes occurring upon diet switch with an increase in abundance of Parabacteroides, Blautia, Clostridium cluster IV, and Phascolarctobacterium.					
30867067	4	149	theme	community	753:761	arg1	dwellers					763:770	elderly community dwellers	745:770	elderly community dwellers	745:770	METHODS Mice were colonized with human microbiota and then underwent an 8-week dietary intervention with either a high-fiber/low-fat diet typical of elderly community dwellers or a low-fiber/high-fat diet typical of long-stay residential care subjects.					
30867067	11	150	theme	inoculated	1829:1838	arg1	mice					1840:1843	community inoculated mice	1819:1843	community inoculated mice	1819:1843	The dominance of Prevotella present initially in community inoculated mice was replaced by Bacteroides, Alistipes, and Oscillibacter.					
31753385	4	0	theme	composite	911:919	arg1	microspheres					929:940	the CS/GO composite aerogel microspheres	901:940	the CS/GO composite aerogel microspheres	901:940	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	4	1	theme	chemical	1114:1121	arg1	processes					1123:1131	both physical and chemical processes	1096:1131	both physical and chemical processes	1096:1131	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	3	2	theme	aerogel	594:600	arg1	microspheres					602:613	the composite aerogel microspheres	580:613	the composite aerogel microspheres (10% GO)	580:622	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	3	2	theme	aerogel	594:600	arg1	%					618:618	10% GO	616:621	10% GO	616:621	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	8	3	dep	rapid	1615:1619	arg1	high					1622:1625	high	1622:1625	high	1622:1625	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	4	4	theme	bilirubin	888:896	arg1	mechanism					875:883	the adsorption mechanism	860:883	the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres	860:940	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	3	5	theme	aerogel	752:758	arg1	microspheres					760:771	the aerogel microspheres	748:771	the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min	748:848	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	2	6	theme	chemical	311:318	arg1	structure					320:328	chemical structure	311:328	chemical structure	311:328	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	1	7	theme	CO2	205:207	arg1	drying					223:228	CO2 supercritical drying	205:228	CO2 supercritical drying	205:228	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	7	8	theme	blood	1518:1522	arg1	compatibility					1524:1536	the good blood compatibility	1509:1536	the good blood compatibility for the adsorbents	1509:1555	In addition, the hemolysis rate and coagulation time tests presented the good blood compatibility for the adsorbents.					
31753385	2	9	theme	microspheres	357:368	arg1	morphology					296:305	morphology	296:305	morphology	296:305	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	2	9	theme	microspheres	357:368	arg1	structure					320:328	chemical structure	311:328	chemical structure	311:328	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	4	10	theme	model	980:984	arg1	fitting					986:992	the relevant model fitting	967:992	the relevant model fitting	967:992	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	3	11	theme	more	787:790	arg1	bilirubin					792:800	much more bilirubin	782:800	much more bilirubin	782:800	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	2	12	theme	composite	339:347	arg1	microspheres					357:368	CS/GO composite aerogel microspheres	333:368	CS/GO composite aerogel microspheres	333:368	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	1	13	theme	excellent	247:255	arg1	performance					257:267	excellent performance	247:267	excellent performance for bilirubin removal	247:289	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	7	14	theme	coagulation	1476:1486	arg1	tests					1493:1497	coagulation time tests	1476:1497	coagulation time tests	1476:1497	In addition, the hemolysis rate and coagulation time tests presented the good blood compatibility for the adsorbents.					
31753385	3	15	theme	shorter	809:815	arg1	time					829:832	a shorter equilibrium time	807:832	a shorter equilibrium time of about 30 min	807:848	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	6	16	theme	adsorption	1419:1428	arg1	capacity					1430:1437	large adsorption capacity	1413:1437	large adsorption capacity	1413:1437	After five adsorption-desorption cycles, the adsorption capacity still maintains large adsorption capacity.					
31753385	1	17	theme	CS/GO	147:151	arg1	microspheres					172:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	8	18	theme	composite	1579:1587	arg1	promising					1685:1693	promising	1685:1693	promising	1685:1693	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	8	18	theme	composite	1579:1587	arg1	microspheres					1597:1608	the CS/GO composite aerogel microspheres	1569:1608	the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility	1569:1674	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	3	19	theme	composite	584:592	arg1	microspheres					602:613	the composite aerogel microspheres	580:613	the composite aerogel microspheres (10% GO)	580:622	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	3	19	theme	composite	584:592	arg1	%					618:618	10% GO	616:621	10% GO	616:621	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	2	20	with	distribution	508:519	arg1	area					463:466	a maximum specific surface area	436:466	a maximum specific surface area of 174.69 m2/g	436:481	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	1	21	theme	composite	154:162	arg1	microspheres					172:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	2	22	theme	good	541:544	arg1	property					557:564	a good mechanical property	539:564	also a good mechanical property	534:564	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	2	22	theme	good	541:544	arg1	20-40 nm					524:531	20-40 nm	524:531	20-40 nm	524:531	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	0	23	theme	high	75:78	arg1	performance					98:108	high bilirubin removal performance	75:108	high bilirubin removal performance	75:108	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.					
31753385	3	24	theme	adsorption	641:650	arg1	178.25 mg/g					662:672	178.25 mg/g	662:672	178.25 mg/g	662:672	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	3	24	theme	adsorption	641:650	arg1	capacity					652:659	a large adsorption capacity	633:659	a large adsorption capacity (178.25 mg/g) for bilirubin	633:687	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	8	25	theme	rapid	1615:1619	arg1	capacity					1638:1645	rapid, high adsorption capacity	1615:1645	rapid, high adsorption capacity	1615:1645	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	7	26	attach	presented	1499:1507	arg2	tests					1493:1497	coagulation time tests	1476:1497	coagulation time tests	1476:1497	In addition, the hemolysis rate and coagulation time tests presented the good blood compatibility for the adsorbents.					
31753385	7	26	attach	presented	1499:1507	arg1	addition					1443:1450	addition	1443:1450	addition	1443:1450	In addition, the hemolysis rate and coagulation time tests presented the good blood compatibility for the adsorbents.					
31753385	7	26	attach	presented	1499:1507	arg2	rate					1467:1470	the hemolysis rate	1453:1470	the hemolysis rate	1453:1470	In addition, the hemolysis rate and coagulation time tests presented the good blood compatibility for the adsorbents.					
31753385	0	27	theme	removal	90:96	arg1	performance					98:108	high bilirubin removal performance	75:108	high bilirubin removal performance	75:108	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.					
31753385	3	28	dep	%	618:618	arg1	GO					620:621	GO	620:621	GO	620:621	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	4	29	theme	adsorption	1005:1014	arg1	kinetics					1016:1023	adsorption kinetics	1005:1023	adsorption kinetics	1005:1023	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	2	30	theme	surface	455:461	arg1	area					463:466	a maximum specific surface area	436:466	a maximum specific surface area of 174.69 m2/g	436:481	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	2	31	theme	pore	498:501	arg1	distribution					508:519	a special pore size distribution	488:519	a special pore size distribution at 20-40 nm, also a good mechanical property	488:564	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	8	32	theme	good	1651:1654	arg1	compatibility					1662:1674	good blood compatibility	1651:1674	good blood compatibility	1651:1674	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	2	33	theme	maximum	438:444	arg1	area					463:466	a maximum specific surface area	436:466	a maximum specific surface area of 174.69 m2/g	436:481	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	1	34	theme	Functional	111:120	arg1	microspheres					172:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	0	35	theme	chitosan/graphene	15:31	arg1	microspheres					57:68	chitosan/graphene oxide composite aerogel microspheres	15:68	chitosan/graphene oxide composite aerogel microspheres	15:68	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.					
31753385	5	36	theme	Freundlich	1270:1279	arg1	isotherm					1281:1288	Freundlich isotherm	1270:1288	Freundlich isotherm	1270:1288	Adsorption isotherm indicated that bilirubin adsorption by the microspheres was fitted well with Freundlich isotherm, which ascribed to multilayer adsorption.					
31753385	1	37	theme	oxide	140:144	arg1	microspheres					172:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	0	38	theme	composite	39:47	arg1	microspheres					57:68	chitosan/graphene oxide composite aerogel microspheres	15:68	chitosan/graphene oxide composite aerogel microspheres	15:68	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.					
31753385	0	39	theme	microspheres	57:68	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.	0:109	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.					
31753385	3	40	theme	adsorption	709:718	arg1	experiment					720:729	2 h. Dynamic adsorption experiment	696:729	2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min	696:848	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	0	41	with	Fabrication	0:10	arg1	performance					98:108	high bilirubin removal performance	75:108	high bilirubin removal performance	75:108	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.					
31753385	5	42	theme	multilayer	1309:1318	arg1	adsorption					1320:1329	multilayer adsorption	1309:1329	multilayer adsorption	1309:1329	Adsorption isotherm indicated that bilirubin adsorption by the microspheres was fitted well with Freundlich isotherm, which ascribed to multilayer adsorption.					
31753385	3	43	theme	2 h.	696:699	arg1	experiment					720:729	2 h. Dynamic adsorption experiment	696:729	2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min	696:848	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	1	44	theme	bilirubin	273:281	arg1	removal					283:289	bilirubin removal	273:289	bilirubin removal	273:289	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	2	45	theme	nanoporous	410:419	arg1	structure					421:429	a nanoporous structure	408:429	a nanoporous structure with a maximum specific surface area of 174.69 m2/g	408:481	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	4	46	theme	CS/GO	905:909	arg1	microspheres					929:940	the CS/GO composite aerogel microspheres	901:940	the CS/GO composite aerogel microspheres	901:940	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	6	47	theme	adsorption	1377:1386	arg1	capacity					1388:1395	the adsorption capacity	1373:1395	the adsorption capacity	1373:1395	After five adsorption-desorption cycles, the adsorption capacity still maintains large adsorption capacity.					
31753385	2	48	theme	CS/GO	333:337	arg1	microspheres					357:368	CS/GO composite aerogel microspheres	333:368	CS/GO composite aerogel microspheres	333:368	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	4	49	theme	aerogel	921:927	arg1	microspheres					929:940	the CS/GO composite aerogel microspheres	901:940	the CS/GO composite aerogel microspheres	901:940	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	6	50	theme	adsorption-desorption	1343:1363	arg1	cycles					1365:1370	five adsorption-desorption cycles	1338:1370	five adsorption-desorption cycles	1338:1370	After five adsorption-desorption cycles, the adsorption capacity still maintains large adsorption capacity.					
31753385	1	51	theme	supercritical	209:221	arg1	drying					223:228	CO2 supercritical drying	205:228	CO2 supercritical drying	205:228	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	2	52	dep	morphology	296:305	arg1	The					292:294	The	292:294	The	292:294	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	2	53	theme	aerogel	349:355	arg1	microspheres					357:368	CS/GO composite aerogel microspheres	333:368	CS/GO composite aerogel microspheres	333:368	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	4	54	theme	relevant	971:978	arg1	fitting					986:992	the relevant model fitting	967:992	the relevant model fitting	967:992	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	7	55	theme	good	1513:1516	arg1	compatibility					1524:1536	the good blood compatibility	1509:1536	the good blood compatibility for the adsorbents	1509:1555	In addition, the hemolysis rate and coagulation time tests presented the good blood compatibility for the adsorbents.					
31753385	8	56	theme	CS/GO	1573:1577	arg1	promising					1685:1693	promising	1685:1693	promising	1685:1693	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	8	56	theme	CS/GO	1573:1577	arg1	microspheres					1597:1608	the CS/GO composite aerogel microspheres	1569:1608	the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility	1569:1674	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	8	57	theme	aerogel	1589:1595	arg1	promising					1685:1693	promising	1685:1693	promising	1685:1693	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	8	57	theme	aerogel	1589:1595	arg1	microspheres					1597:1608	the CS/GO composite aerogel microspheres	1569:1608	the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility	1569:1674	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	7	58	theme	time	1488:1491	arg1	tests					1493:1497	coagulation time tests	1476:1497	coagulation time tests	1476:1497	In addition, the hemolysis rate and coagulation time tests presented the good blood compatibility for the adsorbents.					
31753385	3	59	theme	equilibrium	817:827	arg1	time					829:832	a shorter equilibrium time	807:832	a shorter equilibrium time of about 30 min	807:848	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	6	60	theme	large	1413:1417	arg1	capacity					1430:1437	large adsorption capacity	1413:1437	large adsorption capacity	1413:1437	After five adsorption-desorption cycles, the adsorption capacity still maintains large adsorption capacity.					
31753385	0	61	theme	bilirubin	80:88	arg1	performance					98:108	high bilirubin removal performance	75:108	high bilirubin removal performance	75:108	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.					
31753385	3	62	theme	large	635:639	arg1	178.25 mg/g					662:672	178.25 mg/g	662:672	178.25 mg/g	662:672	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	3	62	theme	large	635:639	arg1	capacity					652:659	a large adsorption capacity	633:659	a large adsorption capacity (178.25 mg/g) for bilirubin	633:687	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	1	63	theme	aerogel	164:170	arg1	microspheres					172:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	4	64	theme	adsorption	1029:1038	arg1	isotherm					1040:1047	adsorption isotherm	1029:1047	adsorption isotherm	1029:1047	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	7	65	theme	hemolysis	1457:1465	arg1	rate					1467:1470	the hemolysis rate	1453:1470	the hemolysis rate	1453:1470	In addition, the hemolysis rate and coagulation time tests presented the good blood compatibility for the adsorbents.					
31753385	3	66	theme	30 min	843:848	arg1	time					829:832	a shorter equilibrium time	807:832	a shorter equilibrium time of about 30 min	807:848	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	5	67	theme	bilirubin	1208:1216	arg1	adsorption					1218:1227	bilirubin adsorption	1208:1227	bilirubin adsorption by the microspheres	1208:1247	Adsorption isotherm indicated that bilirubin adsorption by the microspheres was fitted well with Freundlich isotherm, which ascribed to multilayer adsorption.					
31753385	8	68	theme	adsorption	1627:1636	arg1	capacity					1638:1645	rapid, high adsorption capacity	1615:1645	rapid, high adsorption capacity	1615:1645	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	5	69	theme	Adsorption	1173:1182	arg1	isotherm					1184:1191	Adsorption isotherm	1173:1191	Adsorption isotherm	1173:1191	Adsorption isotherm indicated that bilirubin adsorption by the microspheres was fitted well with Freundlich isotherm, which ascribed to multilayer adsorption.					
31753385	2	70	theme	mechanical	546:555	arg1	property					557:564	a good mechanical property	539:564	also a good mechanical property	534:564	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	2	70	theme	mechanical	546:555	arg1	20-40 nm					524:531	20-40 nm	524:531	20-40 nm	524:531	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	2	71	from	20-40 nm	524:531	arg1	distribution					508:519	a special pore size distribution	488:519	a special pore size distribution at 20-40 nm, also a good mechanical property	488:564	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	2	71	from	20-40 nm	524:531	arg1	structure					421:429	a nanoporous structure	408:429	a nanoporous structure with a maximum specific surface area of 174.69 m2/g	408:481	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	2	72	theme	specific	446:453	arg1	area					463:466	a maximum specific surface area	436:466	a maximum specific surface area of 174.69 m2/g	436:481	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	2	73	theme	special	490:496	arg1	distribution					508:519	a special pore size distribution	488:519	a special pore size distribution at 20-40 nm, also a good mechanical property	488:564	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	0	74	theme	oxide	33:37	arg1	microspheres					57:68	chitosan/graphene oxide composite aerogel microspheres	15:68	chitosan/graphene oxide composite aerogel microspheres	15:68	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.					
31753385	1	75	theme	chitosan/graphene	122:138	arg1	microspheres					172:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres	111:183	Functional chitosan/graphene oxide (CS/GO) composite aerogel microspheres were fabricated via CO2 supercritical drying, which displayed excellent performance for bilirubin removal.					
31753385	4	76	theme	physical	1101:1108	arg1	processes					1123:1131	both physical and chemical processes	1096:1131	both physical and chemical processes	1096:1131	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	2	77	theme	174.69 m2/g	471:481	arg1	area					463:466	a maximum specific surface area	436:466	a maximum specific surface area of 174.69 m2/g	436:481	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	0	78	theme	aerogel	49:55	arg1	microspheres					57:68	chitosan/graphene oxide composite aerogel microspheres	15:68	chitosan/graphene oxide composite aerogel microspheres	15:68	Fabrication of chitosan/graphene oxide composite aerogel microspheres with high bilirubin removal performance.					
31753385	8	79	theme	hyperbilirubinemia	1699:1716	arg1	treatment					1718:1726	hyperbilirubinemia treatment	1699:1726	hyperbilirubinemia treatment	1699:1726	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	8	80	theme	blood	1656:1660	arg1	compatibility					1662:1674	good blood compatibility	1651:1674	good blood compatibility	1651:1674	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	2	81	theme	size	503:506	arg1	distribution					508:519	a special pore size distribution	488:519	a special pore size distribution at 20-40 nm, also a good mechanical property	488:564	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	3	82	theme	Dynamic	701:707	arg1	experiment					720:729	2 h. Dynamic adsorption experiment	696:729	2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min	696:848	Importantly, the composite aerogel microspheres (10% GO) revealed a large adsorption capacity (178.25 mg/g) for bilirubin within 2 h. Dynamic adsorption experiment illustrated that the aerogel microspheres adsorbed much more bilirubin with a shorter equilibrium time of about 30 min.					
31753385	4	83	theme	adsorption	864:873	arg1	mechanism					875:883	the adsorption mechanism	860:883	the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres	860:940	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	4	84	theme	chemical	1138:1145	arg1	adsorption					1147:1156	chemical adsorption	1138:1156	chemical adsorption	1138:1156	Besides, the adsorption mechanism of bilirubin by the CS/GO composite aerogel microspheres was investigated through the relevant model fitting, including adsorption kinetics and adsorption isotherm, which illustrated that the mechanism included both physical and chemical processes, but chemical adsorption was dominated.					
31753385	2	85	with	structure	421:429	arg1	area					463:466	a maximum specific surface area	436:466	a maximum specific surface area of 174.69 m2/g	436:481	The morphology and chemical structure of CS/GO composite aerogel microspheres were characterized, which illustrated a nanoporous structure with a maximum specific surface area of 174.69 m2/g, and a special pore size distribution at 20-40 nm, also a good mechanical property.					
31753385	8	86	with	microspheres	1597:1608	arg1	compatibility					1662:1674	good blood compatibility	1651:1674	good blood compatibility	1651:1674	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31753385	8	86	with	microspheres	1597:1608	arg1	capacity					1638:1645	rapid, high adsorption capacity	1615:1645	rapid, high adsorption capacity	1615:1645	Therefore, the CS/GO composite aerogel microspheres with rapid, high adsorption capacity and good blood compatibility might be promising for hyperbilirubinemia treatment.					
31800608	7	0	from	families	1647:1654	arg1	bacteria					1595:1602	Acetogenic bacteria	1584:1602	Acetogenic bacteria from the Rhizobiaceae and Acetobacteraceae families	1584:1654	Acetogenic bacteria from the Rhizobiaceae and Acetobacteraceae families increased two- to five-fold when bees were fed sucrose.					
31800608	4	1	theme	antibacterial	869:881	arg1	properties					883:892	known antibacterial properties	863:892	known antibacterial properties	863:892	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	0	2	theme	adult	133:137	arg1	bees					145:148	adult honey bees	133:148	adult honey bees (Apis mellifera)	133:165	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	2	theme	adult	133:137	arg1	mellifera					156:164	Apis mellifera	151:164	Apis mellifera	151:164	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	5	3	theme	dominant	1245:1252	arg1	bacteria					1259:1266	dominant core bacteria	1245:1266	dominant core bacteria	1245:1266	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	4	4	from	colony	798:803	arg1	diets					818:822	two natural diets	806:822	two natural diets	806:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	4	from	colony	798:803	arg1	bees					779:782	feeding bees	771:782	feeding bees from a single colony	771:803	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	0	5	theme	bees	145:148	arg1	tract					124:128	the digestive tract	110:128	the digestive tract of adult honey bees (Apis mellifera)	110:165	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	5	6	theme	bacteria	1259:1266	arg1	abundance					1232:1240	the relative abundance	1219:1240	the relative abundance of dominant core bacteria	1219:1266	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	1	7	theme	honey	213:217	arg1	bees					219:222	adult honey bees	207:222	adult honey bees	207:222	Bacteria within the digestive tract of adult honey bees are likely to play a key role in the digestion of sugar-rich foods.					
31800608	7	8	theme	Acetobacteraceae	1630:1645	arg1	families					1647:1654	the Rhizobiaceae and Acetobacteraceae families	1609:1654	families	1647:1654	Acetogenic bacteria from the Rhizobiaceae and Acetobacteraceae families increased two- to five-fold when bees were fed sucrose.					
31800608	4	9	theme	invert	936:941	arg1	sugar					943:947	invert sugar	936:947	invert sugar	936:947	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	3	10	theme	invert	591:596	arg1	sugar					598:602	invert sugar	591:602	invert sugar (a mixture of the monosaccharides glucose and fructose)	591:658	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	0	11	theme	Apis	151:154	arg1	bees					145:148	adult honey bees	133:148	adult honey bees (Apis mellifera)	133:165	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	11	theme	Apis	151:154	arg1	mellifera					156:164	Apis mellifera	151:164	Apis mellifera	151:164	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	4	12	theme	relative	718:725	arg1	abundance					727:735	the relative abundance	714:735	the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	714:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	7	13	theme	Rhizobiaceae	1613:1624	arg1	families					1647:1654	the Rhizobiaceae and Acetobacteraceae families	1609:1654	families	1647:1654	Acetogenic bacteria from the Rhizobiaceae and Acetobacteraceae families increased two- to five-fold when bees were fed sucrose.					
31800608	8	14	theme	primary	1797:1803	arg1	sucrose-feeders					1805:1819	specific low abundance primary sucrose-feeders	1774:1819	specific low abundance primary sucrose-feeders	1774:1819	These results suggest that sucrose fuels the proliferation of specific low abundance primary sucrose-feeders, which metabolise sugars into monosaccharides, and then to acetate.					
31800608	3	15	theme	honey	482:486	arg1	bees					488:491	honey bees	482:491	honey bees	482:491	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	4	16	theme	feeding	771:777	arg1	diets					818:822	two natural diets	806:822	two natural diets	806:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	16	theme	feeding	771:777	arg1	bees					779:782	feeding bees	771:782	feeding bees from a single colony	771:803	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	3	17	theme	carbohydrate	464:475	arg1	sources					453:459	the natural sources	441:459	the natural sources of carbohydrate	441:475	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	3	18	theme	fructose	650:657	arg1	mixture					607:613	a mixture	605:613	a mixture of the monosaccharides glucose and fructose	605:657	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	8	19	theme	specific	1774:1781	arg1	sucrose-feeders					1805:1819	specific low abundance primary sucrose-feeders	1774:1819	specific low abundance primary sucrose-feeders	1774:1819	These results suggest that sucrose fuels the proliferation of specific low abundance primary sucrose-feeders, which metabolise sugars into monosaccharides, and then to acetate.					
31800608	6	20	dep	Frischella	1505:1514	arg1	altered					1549:1555	altered	1549:1555	altered the bacterial composition	1549:1581	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	20	dep	Frischella	1505:1514	arg1	perrara					1516:1522	Frischella perrara, all which significantly altered the bacterial composition	1505:1581	perrara	1516:1522	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	21	theme	Frischella	1505:1514	arg1	abundance					1492:1500	the relative abundance	1479:1500	the relative abundance of Frischella perrara, all which significantly altered the bacterial composition	1479:1581	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	4	22	theme	solutions	980:988	arg1	diets					927:931	artificial diets	916:931	artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey	916:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	3	23	theme	glucose	638:644	arg1	mixture					607:613	a mixture	605:613	a mixture of the monosaccharides glucose and fructose	605:657	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	8	24	theme	abundance	1787:1795	arg1	sucrose-feeders					1805:1819	specific low abundance primary sucrose-feeders	1774:1819	specific low abundance primary sucrose-feeders	1774:1819	These results suggest that sucrose fuels the proliferation of specific low abundance primary sucrose-feeders, which metabolise sugars into monosaccharides, and then to acetate.					
31800608	4	25	from	diet	906:909	arg1	abundance					727:735	the relative abundance	714:735	the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	714:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	26	theme	bees	763:766	arg1	gut					756:758	the gut	752:758	the gut of bees by feeding bees from a single colony, two natural diets	752:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	27	theme	synthesised	1001:1011	arg1	compounds					1013:1021	synthesised compounds	1001:1021	synthesised compounds associated with the antibacterial properties of mānuka honey	1001:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	6	28	theme	bacteria	1396:1403	arg1	abundances					1358:1367	the relative abundances	1345:1367	the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei,	1345:1463	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	29	theme	core	1391:1394	arg1	Acetobacteraceae					1420:1435	Acetobacteraceae	1420:1435	Acetobacteraceae	1420:1435	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	29	theme	core	1391:1394	arg1	Rhizobiaceae					1406:1417	Rhizobiaceae	1406:1417	Rhizobiaceae	1406:1417	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	29	theme	core	1391:1394	arg1	kunkeei					1456:1462	Lactobacillus kunkeei	1442:1462	Lactobacillus kunkeei	1442:1462	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	29	theme	core	1391:1394	arg1	bacteria					1396:1403	three sub-dominant core bacteria	1372:1403	three sub-dominant core bacteria	1372:1403	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	4	30	theme	natural	810:816	arg1	bees					779:782	feeding bees	771:782	feeding bees from a single colony	771:803	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	30	theme	natural	810:816	arg1	diets					818:822	two natural diets	806:822	two natural diets	806:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	31	theme	solution	958:965	arg1	diets					927:931	artificial diets	916:931	artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey	916:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	5	32	theme	-based	1109:1114	arg1	sequencing					1116:1125	16S ribosomal RNA (rRNA)-based sequencing	1085:1125	16S ribosomal RNA (rRNA)-based sequencing	1085:1125	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	0	33	theme	invert	45:50	arg1	sugar					52:56	invert sugar	45:56	invert sugar	45:56	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	33	theme	invert	45:50	arg1	sources					27:33	carbohydrate sources	14:33	carbohydrate sources: Sucrose, invert sugar and components of mānuka honey	14:87	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	8	34	theme	sucrose-feeders	1805:1819	arg1	proliferation					1757:1769	the proliferation	1753:1769	the proliferation of specific low abundance primary sucrose-feeders, which metabolise sugars into monosaccharides	1753:1865	These results suggest that sucrose fuels the proliferation of specific low abundance primary sucrose-feeders, which metabolise sugars into monosaccharides, and then to acetate.					
31800608	5	35	theme	16S	1085:1087	arg1	rRNA					1104:1107	rRNA	1104:1107	rRNA	1104:1107	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	5	35	theme	16S	1085:1087	arg1	RNA					1099:1101	16S ribosomal RNA	1085:1101	16S ribosomal RNA (rRNA)-based sequencing	1085:1125	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	4	36	with	diet	906:909	arg1	properties					883:892	known antibacterial properties	863:892	known antibacterial properties	863:892	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	37	theme	single	791:796	arg1	colony					798:803	a single colony	789:803	a single colony	789:803	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	6	38	theme	bacterial	1561:1569	arg1	composition					1571:1581	the bacterial composition	1557:1581	the bacterial composition	1557:1581	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	5	39	theme	RNA	1099:1101	arg1	sequencing					1116:1125	16S ribosomal RNA (rRNA)-based sequencing	1085:1125	16S ribosomal RNA (rRNA)-based sequencing	1085:1125	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	1	40	theme	sugar-rich	274:283	arg1	foods					285:289	sugar-rich foods	274:289	sugar-rich foods	274:289	Bacteria within the digestive tract of adult honey bees are likely to play a key role in the digestion of sugar-rich foods.					
31800608	4	41	theme	mānuka	1071:1076	arg1	honey					1078:1082	mānuka honey	1071:1082	mānuka honey	1071:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	0	42	theme	honey	83:87	arg1	components					62:71	components	62:71	components of mānuka honey	62:87	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	42	theme	honey	83:87	arg1	Sucrose					36:42	Sucrose	36:42	Sucrose	36:42	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	42	theme	honey	83:87	arg1	sugar					52:56	invert sugar	45:56	invert sugar	45:56	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	42	theme	honey	83:87	arg1	sources					27:33	carbohydrate sources	14:33	carbohydrate sources: Sucrose, invert sugar and components of mānuka honey	14:87	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	6	43	theme	Lactobacillus	1442:1454	arg1	bacteria					1396:1403	three sub-dominant core bacteria	1372:1403	three sub-dominant core bacteria	1372:1403	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	43	theme	Lactobacillus	1442:1454	arg1	kunkeei					1456:1462	Lactobacillus kunkeei	1442:1462	Lactobacillus kunkeei	1442:1462	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	5	44	theme	honey	1173:1177	arg1	sugar					1199:1203	mānuka honey, sucrose and invert sugar	1166:1203	sugar	1199:1203	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	1	45	theme	bees	219:222	arg1	tract					198:202	the digestive tract	184:202	the digestive tract of adult honey bees	184:222	Bacteria within the digestive tract of adult honey bees are likely to play a key role in the digestion of sugar-rich foods.					
31800608	4	46	theme	known	863:867	arg1	properties					883:892	known antibacterial properties	863:892	known antibacterial properties	863:892	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	2	47	theme	bee	332:334	arg1	bacteria					340:347	honey bee gut bacteria	326:347	honey bee gut bacteria	326:347	However, the influence of diet on honey bee gut bacteria is not well understood.					
31800608	4	48	from	honey	852:856	arg1	abundance					727:735	the relative abundance	714:735	the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	714:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	3	49	theme	carbohydrates	540:552	arg1	forms					531:535	various forms	523:535	various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose)	523:658	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	5	50	theme	dietary	1139:1145	arg1	regimes					1147:1153	dietary regimes	1139:1153	dietary regimes containing mānuka honey, sucrose and invert sugar	1139:1203	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	0	51	theme	sources	27:33	arg1	effect					4:9	The effect	0:9	The effect	0:9	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	4	52	theme	sugar	693:697	arg1	supplements					699:709	these sugar supplements	687:709	these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	687:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	3	53	theme	low	391:393	arg1	abundance					402:410	low floral abundance	391:410	low floral abundance	391:410	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	4	54	from	honey	832:836	arg1	abundance					727:735	the relative abundance	714:735	the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	714:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	0	55	dep	sources	27:33	arg1	components					62:71	components	62:71	components of mānuka honey	62:87	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	55	dep	sources	27:33	arg1	Sucrose					36:42	Sucrose	36:42	Sucrose	36:42	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	55	dep	sources	27:33	arg1	sugar					52:56	invert sugar	45:56	invert sugar	45:56	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	55	dep	sources	27:33	arg1	sources					27:33	carbohydrate sources	14:33	carbohydrate sources: Sucrose, invert sugar and components of mānuka honey	14:87	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	4	56	theme	hive	901:904	arg1	diet					906:909	a hive diet	899:909	a hive diet	899:909	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	0	57	from	bacteria	98:105	arg1	tract					124:128	the digestive tract	110:128	the digestive tract of adult honey bees (Apis mellifera)	110:165	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	5	58	theme	sucrose	1180:1186	arg1	sugar					1199:1203	mānuka honey, sucrose and invert sugar	1166:1203	sugar	1199:1203	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	0	59	theme	honey	139:143	arg1	bees					145:148	adult honey bees	133:148	adult honey bees (Apis mellifera)	133:165	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	59	theme	honey	139:143	arg1	mellifera					156:164	Apis mellifera	151:164	Apis mellifera	151:164	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	5	60	theme	invert	1192:1197	arg1	sugar					1199:1203	mānuka honey, sucrose and invert sugar	1166:1203	sugar	1199:1203	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	3	61	theme	abundance	402:410	arg1	periods					380:386	periods	380:386	periods of low floral abundance	380:410	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	5	62	theme	core	1254:1257	arg1	bacteria					1259:1266	dominant core bacteria	1245:1266	dominant core bacteria	1245:1266	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	3	63	theme	natural	445:451	arg1	sources					453:459	the natural sources	441:459	the natural sources of carbohydrate	441:475	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	1	64	theme	adult	207:211	arg1	bees					219:222	adult honey bees	207:222	adult honey bees	207:222	Bacteria within the digestive tract of adult honey bees are likely to play a key role in the digestion of sugar-rich foods.					
31800608	4	65	theme	bacteria	740:747	arg1	abundance					727:735	the relative abundance	714:735	the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	714:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	66	with	honey	852:856	arg1	properties					883:892	known antibacterial properties	863:892	known antibacterial properties	863:892	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	67	theme	sugar	943:947	arg1	diets					927:931	artificial diets	916:931	artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey	916:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	5	68	contain	containing	1155:1164	arg2	sugar					1199:1203	mānuka honey, sucrose and invert sugar	1166:1203	sugar	1199:1203	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	5	68	contain	containing	1155:1164	arg1	regimes					1147:1153	dietary regimes	1139:1153	dietary regimes containing mānuka honey, sucrose and invert sugar	1139:1203	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	6	69	theme	relative	1349:1356	arg1	abundances					1358:1367	the relative abundances	1345:1367	the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei,	1345:1463	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	4	70	contain	containing	990:999	arg1	diets					927:931	artificial diets	916:931	artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey	916:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	70	contain	containing	990:999	arg2	compounds					1013:1021	synthesised compounds	1001:1021	synthesised compounds associated with the antibacterial properties of mānuka honey	1001:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	71	theme	supplements	699:709	arg1	honey					852:856	a monofloral honey	839:856	a monofloral honey with known antibacterial properties	839:892	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	71	theme	supplements	699:709	arg1	diet					906:909	a hive diet	899:909	a hive diet	899:909	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	71	theme	supplements	699:709	arg1	honey					832:836	mānuka honey	825:836	mānuka honey	825:836	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	71	theme	supplements	699:709	arg1	effect					677:682	the effect	673:682	the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	673:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	72	theme	sucrose	950:956	arg1	solution					958:965	sucrose solution	950:965	sucrose solution	950:965	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	1	73	theme	key	245:247	arg1	role					249:252	a key role	243:252	a key role	243:252	Bacteria within the digestive tract of adult honey bees are likely to play a key role in the digestion of sugar-rich foods.					
31800608	5	74	theme	relative	1223:1230	arg1	abundance					1232:1240	the relative abundance	1219:1240	the relative abundance of dominant core bacteria	1219:1266	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	4	75	with	honey	832:836	arg1	properties					883:892	known antibacterial properties	863:892	known antibacterial properties	863:892	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	76	theme	artificial	916:925	arg1	diets					927:931	artificial diets	916:931	artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey	916:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	3	77	theme	monosaccharides	622:636	arg1	glucose					638:644	the monosaccharides glucose	618:644	the monosaccharides glucose	618:644	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	0	78	theme	core	93:96	arg1	bacteria					98:105	core bacteria	93:105	core bacteria in the digestive tract of adult honey bees (Apis mellifera)	93:165	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	6	79	theme	sucrose-rich	1316:1327	arg1	diets					1329:1333	sucrose-rich diets	1316:1333	sucrose-rich diets	1316:1333	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	7	80	dep	five-fold	1674:1682	arg1	to					1671:1672	to	1671:1672	to	1671:1672	Acetogenic bacteria from the Rhizobiaceae and Acetobacteraceae families increased two- to five-fold when bees were fed sucrose.					
31800608	8	81	theme	low	1783:1785	arg1	sucrose-feeders					1805:1819	specific low abundance primary sucrose-feeders	1774:1819	specific low abundance primary sucrose-feeders	1774:1819	These results suggest that sucrose fuels the proliferation of specific low abundance primary sucrose-feeders, which metabolise sugars into monosaccharides, and then to acetate.					
31800608	0	82	theme	digestive	114:122	arg1	tract					124:128	the digestive tract	110:128	the digestive tract of adult honey bees (Apis mellifera)	110:165	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	6	83	dep	all	1525:1527	arg1	which					1529:1533	which	1529:1533	which	1529:1533	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	4	84	from	effect	677:682	arg1	abundance					727:735	the relative abundance	714:735	the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	714:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	7	85	theme	Acetogenic	1584:1593	arg1	bacteria					1595:1602	Acetogenic bacteria	1584:1602	Acetogenic bacteria from the Rhizobiaceae and Acetobacteraceae families	1584:1654	Acetogenic bacteria from the Rhizobiaceae and Acetobacteraceae families increased two- to five-fold when bees were fed sucrose.					
31800608	6	86	theme	relative	1483:1490	arg1	abundance					1492:1500	the relative abundance	1479:1500	the relative abundance of Frischella perrara, all which significantly altered the bacterial composition	1479:1581	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	87	theme	sub-dominant	1378:1389	arg1	Acetobacteraceae					1420:1435	Acetobacteraceae	1420:1435	Acetobacteraceae	1420:1435	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	87	theme	sub-dominant	1378:1389	arg1	Rhizobiaceae					1406:1417	Rhizobiaceae	1406:1417	Rhizobiaceae	1406:1417	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	87	theme	sub-dominant	1378:1389	arg1	kunkeei					1456:1462	Lactobacillus kunkeei	1442:1462	Lactobacillus kunkeei	1442:1462	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	6	87	theme	sub-dominant	1378:1389	arg1	bacteria					1396:1403	three sub-dominant core bacteria	1372:1403	three sub-dominant core bacteria	1372:1403	However, sucrose-rich diets increased the relative abundances of three sub-dominant core bacteria, Rhizobiaceae, Acetobacteraceae, and Lactobacillus kunkeei, and decreased the relative abundance of Frischella perrara, all which significantly altered the bacterial composition.					
31800608	4	88	theme	sucrose	972:978	arg1	solutions					980:988	sucrose solutions	972:988	sucrose solutions	972:988	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	89	with	effect	677:682	arg1	properties					883:892	known antibacterial properties	863:892	known antibacterial properties	863:892	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	0	90	from	effect	4:9	arg1	bacteria					98:105	core bacteria	93:105	core bacteria in the digestive tract of adult honey bees (Apis mellifera)	93:165	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	5	91	theme	ribosomal	1089:1097	arg1	rRNA					1104:1107	rRNA	1104:1107	rRNA	1104:1107	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	5	91	theme	ribosomal	1089:1097	arg1	RNA					1099:1101	16S ribosomal RNA	1085:1101	16S ribosomal RNA (rRNA)-based sequencing	1085:1125	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	0	92	theme	mānuka	76:81	arg1	honey					83:87	mānuka honey	76:87	mānuka honey	76:87	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	4	93	from	abundance	727:735	arg1	honey					852:856	a monofloral honey	839:856	a monofloral honey with known antibacterial properties	839:892	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	93	from	abundance	727:735	arg1	diet					906:909	a hive diet	899:909	a hive diet	899:909	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	93	from	abundance	727:735	arg1	honey					832:836	mānuka honey	825:836	mānuka honey	825:836	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	93	from	abundance	727:735	arg1	effect					677:682	the effect	673:682	the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	673:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	93	from	abundance	727:735	arg1	gut					756:758	the gut	752:758	the gut of bees by feeding bees from a single colony, two natural diets	752:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	94	theme	honey	1078:1082	arg1	properties					1057:1066	the antibacterial properties	1039:1066	the antibacterial properties of mānuka honey	1039:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	1	95	theme	foods	285:289	arg1	digestion					261:269	the digestion	257:269	the digestion of sugar-rich foods	257:289	Bacteria within the digestive tract of adult honey bees are likely to play a key role in the digestion of sugar-rich foods.					
31800608	5	96	theme	mānuka	1166:1171	arg1	sugar					1199:1203	mānuka honey, sucrose and invert sugar	1166:1203	sugar	1199:1203	16S ribosomal RNA (rRNA)-based sequencing showed that dietary regimes containing mānuka honey, sucrose and invert sugar did not alter the relative abundance of dominant core bacteria after 6 days of being fed these diets.					
31800608	4	97	from	supplements	699:709	arg1	abundance					727:735	the relative abundance	714:735	the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets	714:822	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	98	theme	monofloral	841:850	arg1	honey					852:856	a monofloral honey	839:856	a monofloral honey with known antibacterial properties	839:892	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	4	99	theme	antibacterial	1043:1055	arg1	properties					1057:1066	the antibacterial properties	1039:1066	the antibacterial properties of mānuka honey	1039:1082	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	0	100	theme	carbohydrate	14:25	arg1	Sucrose					36:42	Sucrose	36:42	Sucrose	36:42	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	100	theme	carbohydrate	14:25	arg1	sugar					52:56	invert sugar	45:56	invert sugar	45:56	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	0	100	theme	carbohydrate	14:25	arg1	sources					27:33	carbohydrate sources	14:33	carbohydrate sources: Sucrose, invert sugar and components of mānuka honey	14:87	The effect of carbohydrate sources: Sucrose, invert sugar and components of mānuka honey, on core bacteria in the digestive tract of adult honey bees (Apis mellifera).					
31800608	2	101	theme	gut	336:338	arg1	bacteria					340:347	honey bee gut bacteria	326:347	honey bee gut bacteria	326:347	However, the influence of diet on honey bee gut bacteria is not well understood.					
31800608	2	102	theme	honey	326:330	arg1	bacteria					340:347	honey bee gut bacteria	326:347	honey bee gut bacteria	326:347	However, the influence of diet on honey bee gut bacteria is not well understood.					
31800608	4	103	theme	mānuka	825:830	arg1	honey					832:836	mānuka honey	825:836	mānuka honey	825:836	We compared the effect of these sugar supplements on the relative abundance of bacteria in the gut of bees by feeding bees from a single colony, two natural diets: mānuka honey, a monofloral honey with known antibacterial properties, and a hive diet; and artificial diets of invert sugar, sucrose solution, and sucrose solutions containing synthesised compounds associated with the antibacterial properties of mānuka honey.					
31800608	3	104	theme	various	523:529	arg1	forms					531:535	various forms	523:535	various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose)	523:658	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	2	105	theme	diet	318:321	arg1	influence					305:313	the influence	301:313	the influence of diet on honey bee gut bacteria	301:347	However, the influence of diet on honey bee gut bacteria is not well understood.					
31800608	3	106	dep	sugar	598:602	arg1	mixture					607:613	a mixture	605:613	a mixture of the monosaccharides glucose and fructose	605:657	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	3	107	theme	floral	395:400	arg1	abundance					402:410	low floral abundance	391:410	low floral abundance	391:410	During periods of low floral abundance, beekeepers often supplement the natural sources of carbohydrate that honey bees collect, such as nectar, with various forms of carbohydrates such as sucrose (a disaccharide) and invert sugar (a mixture of the monosaccharides glucose and fructose).					
31800608	2	108	from	influence	305:313	arg1	bacteria					340:347	honey bee gut bacteria	326:347	honey bee gut bacteria	326:347	However, the influence of diet on honey bee gut bacteria is not well understood.					
31800608	1	109	theme	digestive	188:196	arg1	tract					198:202	the digestive tract	184:202	the digestive tract of adult honey bees	184:222	Bacteria within the digestive tract of adult honey bees are likely to play a key role in the digestion of sugar-rich foods.					
29885893	1	0	from	depression	363:372	arg1	cows					377:380	cows	377:380	cows	377:380	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	2	1	theme	fed	662:664	arg1	diets					666:670	fed diets	662:670	fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI)	662:872	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	10	2	theme	milk	2099:2102	arg1	concentration					2104:2116	the milk concentration	2095:2116	the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1	2095:2237	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	9	3	theme	such	1833:1836	arg1	isomers					1824:1830	other conjugated linoleic acid isomers	1793:1830	other conjugated linoleic acid isomers	1793:1830	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	9	4	dep	goats	1964:1968	arg1	fold					1979:1982	4 and 2 fold	1971:1982	4 and 2 fold	1971:1982	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	1	5	theme	cows	219:222	arg1	study					204:208	A direct comparative study	183:208	A direct comparative study of dairy cows and goats	183:232	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	9	6	theme	greater	1898:1904	arg1	extent					1906:1911	a greater extent	1896:1911	a greater extent	1896:1911	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	1	7	theme	goats	228:232	arg1	study					204:208	A direct comparative study	183:208	A direct comparative study of dairy cows and goats	183:232	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	8	8	theme	fold	1708:1711	arg1	increase					1630:1637	an increase	1627:1637	an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1	1627:1741	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	2	9	theme	square	902:907	arg1	design					909:914	a 4 × 4 Latin square design	888:914	a 4 × 4 Latin square design with 28-d experimental periods	888:945	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	0	10	theme	comparative	164:174	arg1	study					176:180	A comparative study	162:180	A comparative study	162:180	Diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil differentially modulate milk fat secretion and composition in cows and goats: A comparative study.					
29885893	7	11	from	FA	1584:1585	arg1	cows					1590:1593	cows	1590:1593	cows	1590:1593	HPO supplementation increased the milk yield of C16 FA in cows.					
29885893	11	12	from	16:0	2372:2375	arg1	cows					2386:2389	cows	2386:2389	cows	2386:2389	Compared with CTL, the response to HPO was distinguished by an increase in 16:0 (10%) in cows.					
29885893	2	13	theme	Holstein	512:519	arg1	DIM					607:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	13	theme	Holstein	512:519	arg1	cows					521:524	Twelve Holstein cows	505:524	Twelve Holstein cows	505:524	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	14	theme	±	601:601	arg1	DIM					607:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	14	theme	±	601:601	arg1	cows					521:524	Twelve Holstein cows	505:524	Twelve Holstein cows	505:524	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	10	15	from	increase	2248:2255	arg1	18:1					2269:2272	trans-10 18:1	2260:2272	trans-10 18:1 only observed in cows	2260:2294	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	7	16	from	cows	1590:1593	arg1	yield					1571:1575	the milk yield	1562:1575	the milk yield of C16 FA in cows	1562:1593	HPO supplementation increased the milk yield of C16 FA in cows.					
29885893	8	17	from	cows	1697:1700	arg1	increase					1630:1637	an increase	1627:1637	an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1	1627:1741	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	10	18	theme	trans	2228:2232	arg1	18:1					2234:2237	the total trans 18:1	2218:2237	the total trans 18:1	2218:2237	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	7	19	theme	HPO	1532:1534	arg1	supplementation					1536:1550	HPO supplementation	1532:1550	HPO supplementation	1532:1550	HPO supplementation increased the milk yield of C16 FA in cows.					
29885893	8	20	theme	18:1	1738:1741	arg1	increase					1630:1637	an increase	1627:1637	an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1	1627:1741	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	2	21	contain	containing	672:681	arg2	diets					712:716	diets	712:716	diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI)	712:872	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	21	contain	containing	672:681	arg2	CTL					704:706	CTL	704:706	CTL	704:706	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	21	contain	containing	672:681	arg2	lipid					697:701	no additional lipid	683:701	no additional lipid (CTL)	683:707	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	21	contain	containing	672:681	arg1	diets					666:670	fed diets	662:670	fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI)	662:872	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	6	22	from	increase	1427:1434	arg1	cows					1468:1471	cows	1468:1471	cows	1468:1471	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	6	22	from	increase	1427:1434	arg1	goats					1450:1454	goats	1450:1454	goats	1450:1454	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	0	23	theme	fat	113:115	arg1	secretion					117:125	milk fat secretion	108:125	milk fat secretion	108:125	Diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil differentially modulate milk fat secretion and composition in cows and goats: A comparative study.					
29885893	10	24	dep	18:1	2157:2160	arg1	fold					2165:2168	2 fold	2163:2168	2 fold	2163:2168	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	3	25	theme	significant	974:984	arg1	effects					986:992	no significant effects	971:992	no significant effects on milk yield and DMI in both species	971:1030	Dietary treatments had no significant effects on milk yield and DMI in both species, except for COS in cows, which decreased DMI by 17%.					
29885893	2	26	theme	algae	805:809	arg1	DMI					829:831	MAP; 1.5% DMI	819:831	MAP; 1.5% DMI	819:831	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	26	theme	algae	805:809	arg1	powder					811:816	marine algae powder	798:816	marine algae powder (MAP; 1.5% DMI)	798:832	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	11	27	from	increase	2360:2367	arg1	16:0					2372:2375	16:0	2372:2375	16:0 (10%) in cows	2372:2389	Compared with CTL, the response to HPO was distinguished by an increase in 16:0 (10%) in cows.					
29885893	11	27	from	increase	2360:2367	arg1	%					2380:2380	10%	2378:2380	10%	2378:2380	Compared with CTL, the response to HPO was distinguished by an increase in 16:0 (10%) in cows.					
29885893	12	28	theme	COS	2487:2489	arg1	treatments					2499:2508	COS and HPO treatments	2487:2508	COS and HPO treatments	2487:2508	This comparative study clearly demonstrated that each ruminant species responds differently to COS and HPO treatments, whereas MAP caused similar effects, and that goats are less sensitive than cows to diets that induce a shift from the trans-11 toward the trans-10 ruminal pathways.					
29885893	1	29	theme	milk	354:357	arg1	fat					359:361	milk fat	354:361	milk fat depression in cows as well as a diet that increases milk fat content in cows	354:438	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	8	30	theme	trans-10	1642:1649	arg1	increase					1630:1637	an increase	1627:1637	an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1	1627:1741	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	12	31	theme	HPO	2495:2497	arg1	treatments					2499:2508	COS and HPO treatments	2487:2508	COS and HPO treatments	2487:2508	This comparative study clearly demonstrated that each ruminant species responds differently to COS and HPO treatments, whereas MAP caused similar effects, and that goats are less sensitive than cows to diets that induce a shift from the trans-11 toward the trans-10 ruminal pathways.					
29885893	0	32	theme	marine	45:50	arg1	algae					52:56	marine algae	45:56	marine algae	45:56	Diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil differentially modulate milk fat secretion and composition in cows and goats: A comparative study.					
29885893	1	33	theme	milk	291:294	arg1	acid					302:305	milk fatty acid	291:305	milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown	291:502	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	1	33	theme	milk	291:294	arg1	FA					308:309	FA	308:309	FA	308:309	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	2	34	theme	±	587:587	arg1	DIM					607:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	34	theme	±	587:587	arg1	cows					521:524	Twelve Holstein cows	505:524	Twelve Holstein cows	505:524	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	1	35	theme	acid	302:305	arg1	responses					312:320	milk fatty acid (FA) responses	291:320	milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown	291:502	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	9	36	theme	conjugated	1799:1808	arg1	isomers					1824:1830	other conjugated linoleic acid isomers	1793:1830	other conjugated linoleic acid isomers	1793:1830	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	0	37	dep	modulate	99:106	arg1	study					176:180	A comparative study	162:180	A comparative study	162:180	Diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil differentially modulate milk fat secretion and composition in cows and goats: A comparative study.					
29885893	2	38	theme	matter	753:758	arg1	DMI					768:770	DMI	768:770	DMI	768:770	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	38	theme	matter	753:758	arg1	intake					760:765	corn oil [5% dry matter intake	736:765	corn oil [5% dry matter intake (DMI)]	736:772	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	0	39	theme	hydrogenated	62:73	arg1	oil					80:82	hydrogenated palm oil	62:82	hydrogenated palm oil	62:82	Diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil differentially modulate milk fat secretion and composition in cows and goats: A comparative study.					
29885893	9	40	theme	acid	1819:1822	arg1	isomers					1824:1830	other conjugated linoleic acid isomers	1793:1830	other conjugated linoleic acid isomers	1793:1830	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	10	41	theme	cis-9	2151:2155	arg1	18:1					2157:2160	cis-9 18:1	2151:2160	cis-9 18:1 (2 fold, on average)	2151:2181	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	2	42	theme	%	867:867	arg1	oil					856:858	hydrogenated palm oil	838:858	hydrogenated palm oil (HPO; 3% DMI)	838:872	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	42	theme	%	867:867	arg1	DMI					869:871	HPO; 3% DMI	861:871	HPO; 3% DMI	861:871	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	43	theme	hydrogenated	838:849	arg1	oil					856:858	hydrogenated palm oil	838:858	hydrogenated palm oil (HPO; 3% DMI)	838:872	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	43	theme	hydrogenated	838:849	arg1	DMI					869:871	HPO; 3% DMI	861:871	HPO; 3% DMI	861:871	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	12	44	theme	ruminal	2658:2664	arg1	pathways					2666:2673	the trans-10 ruminal pathways	2645:2673	the trans-10 ruminal pathways	2645:2673	This comparative study clearly demonstrated that each ruminant species responds differently to COS and HPO treatments, whereas MAP caused similar effects, and that goats are less sensitive than cows to diets that induce a shift from the trans-11 toward the trans-10 ruminal pathways.					
29885893	6	45	theme	>C16	1482:1485	arg1	yield					1490:1494	the >C16 FA yield	1478:1494	the >C16 FA yield	1478:1494	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	8	46	from	trans-10	1642:1649	arg1	cows					1697:1700	cows	1697:1700	cows	1697:1700	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	10	47	dep	18:0	2121:2124	arg1	fold					2129:2132	3 fold	2127:2132	3 fold	2127:2132	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	2	48	theme	HPO	861:863	arg1	oil					856:858	hydrogenated palm oil	838:858	hydrogenated palm oil (HPO; 3% DMI)	838:872	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	48	theme	HPO	861:863	arg1	DMI					869:871	HPO; 3% DMI	861:871	HPO; 3% DMI	861:871	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	4	49	theme	milk	1094:1097	arg1	content					1103:1109	milk fat content	1094:1109	milk fat content	1094:1109	In cows, milk fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (13%) compared with CTL, and in goats only MAP had an effect compared with CTL by decreasing milk fat content by 15%.					
29885893	8	50	from	fold	1768:1771	arg1	goats					1776:1780	goats	1776:1780	goats	1776:1780	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	8	51	from	increase	1630:1637	arg1	goats					1716:1720	goats	1716:1720	goats	1716:1720	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	8	51	from	increase	1630:1637	arg1	cows					1697:1700	cows	1697:1700	cows	1697:1700	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	5	52	dep	yields	1332:1337	arg1	mmol/d					1340:1345	mmol/d	1340:1345	mmol/d	1340:1345	In both species, COS and MAP lowered the yields (mmol/d per kg of BW) of <C16 and C16 FA.					
29885893	3	53	contain	had	967:969	arg2	effects					986:992	no significant effects	971:992	no significant effects on milk yield and DMI in both species	971:1030	Dietary treatments had no significant effects on milk yield and DMI in both species, except for COS in cows, which decreased DMI by 17%.					
29885893	3	53	contain	had	967:969	arg1	treatments					956:965	Dietary treatments	948:965	Dietary treatments	948:965	Dietary treatments had no significant effects on milk yield and DMI in both species, except for COS in cows, which decreased DMI by 17%.					
29885893	7	54	theme	milk	1566:1569	arg1	yield					1571:1575	the milk yield	1562:1575	the milk yield of C16 FA in cows	1562:1593	HPO supplementation increased the milk yield of C16 FA in cows.					
29885893	8	55	theme	linoleic	1669:1676	arg1	acid					1678:1681	cis-12 conjugated linoleic acid	1651:1681	cis-12 conjugated linoleic acid by 18 fold in cows	1651:1700	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	2	56	theme	%	747:747	arg1	DMI					768:770	DMI	768:770	DMI	768:770	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	56	theme	%	747:747	arg1	intake					760:765	corn oil [5% dry matter intake	736:765	corn oil [5% dry matter intake (DMI)]	736:772	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	5	57	theme	FA	1377:1378	arg1	yields					1332:1337	the yields	1328:1337	the yields (mmol/d per kg of BW) of <C16 and C16 FA	1328:1378	In both species, COS and MAP lowered the yields (mmol/d per kg of BW) of <C16 and C16 FA.					
29885893	2	58	theme	MAP	819:821	arg1	DMI					829:831	MAP; 1.5% DMI	819:831	MAP; 1.5% DMI	819:831	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	58	theme	MAP	819:821	arg1	powder					811:816	marine algae powder	798:816	marine algae powder (MAP; 1.5% DMI)	798:832	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	6	59	theme	FA	1444:1445	arg1	increase					1427:1434	an increase	1424:1434	an increase of >C16 FA in goats, but not in cows	1424:1471	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	1	60	from	diet	395:398	arg1	cows					377:380	cows	377:380	cows	377:380	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	2	61	theme	oil	741:743	arg1	DMI					768:770	DMI	768:770	DMI	768:770	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	61	theme	oil	741:743	arg1	intake					760:765	corn oil [5% dry matter intake	736:765	corn oil [5% dry matter intake (DMI)]	736:772	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	4	62	theme	milk	1266:1269	arg1	content					1275:1281	milk fat content	1266:1281	milk fat content	1266:1281	In cows, milk fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (13%) compared with CTL, and in goats only MAP had an effect compared with CTL by decreasing milk fat content by 15%.					
29885893	5	63	theme	BW	1357:1358	arg1	kg					1351:1352	kg	1351:1352	kg of BW	1351:1358	In both species, COS and MAP lowered the yields (mmol/d per kg of BW) of <C16 and C16 FA.					
29885893	7	64	theme	FA	1584:1585	arg1	yield					1571:1575	the milk yield	1562:1575	the milk yield of C16 FA in cows	1562:1593	HPO supplementation increased the milk yield of C16 FA in cows.					
29885893	1	65	theme	fat	420:422	arg1	content					424:430	milk fat content	415:430	milk fat content in cows	415:438	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	4	66	contain	had	1220:1222	arg1	MAP					1216:1218	only MAP	1211:1218	only MAP	1211:1218	In cows, milk fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (13%) compared with CTL, and in goats only MAP had an effect compared with CTL by decreasing milk fat content by 15%.					
29885893	4	66	contain	had	1220:1222	arg2	effect					1227:1232	an effect	1224:1232	an effect	1224:1232	In cows, milk fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (13%) compared with CTL, and in goats only MAP had an effect compared with CTL by decreasing milk fat content by 15%.					
29885893	1	67	theme	direct	185:190	arg1	study					204:208	A direct comparative study	183:208	A direct comparative study of dairy cows and goats	183:232	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	10	68	theme	3-fold	2199:2204	arg1	increase					2206:2213	a 3-fold increase	2197:2213	a 3-fold increase in the total trans 18:1	2197:2237	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	2	69	theme	additional	686:695	arg1	CTL					704:706	CTL	704:706	CTL	704:706	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	69	theme	additional	686:695	arg1	lipid					697:701	no additional lipid	683:701	no additional lipid (CTL)	683:707	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	1	70	theme	dairy	213:217	arg1	cows					219:222	dairy cows	213:222	dairy cows	213:222	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	10	71	theme	total	2222:2226	arg1	18:1					2234:2237	the total trans 18:1	2218:2237	the total trans 18:1	2218:2237	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	10	72	theme	trans-10	2260:2267	arg1	18:1					2269:2272	trans-10 18:1	2260:2272	trans-10 18:1 only observed in cows	2260:2294	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	12	73	theme	similar	2530:2536	arg1	effects					2538:2544	similar effects	2530:2544	similar effects	2530:2544	This comparative study clearly demonstrated that each ruminant species responds differently to COS and HPO treatments, whereas MAP caused similar effects, and that goats are less sensitive than cows to diets that induce a shift from the trans-11 toward the trans-10 ruminal pathways.					
29885893	3	74	from	COS	1044:1046	arg1	cows					1051:1054	cows	1051:1054	cows	1051:1054	Dietary treatments had no significant effects on milk yield and DMI in both species, except for COS in cows, which decreased DMI by 17%.					
29885893	6	75	from	FA	1444:1445	arg1	cows					1468:1471	cows	1468:1471	cows	1468:1471	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	6	75	from	FA	1444:1445	arg1	goats					1450:1454	goats	1450:1454	goats	1450:1454	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	10	76	located	observed	2279:2286	arg1	cows					2291:2294	cows	2291:2294	cows	2291:2294	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	10	76	located	observed	2279:2286	arg2	18:1					2269:2272	trans-10 18:1	2260:2272	trans-10 18:1 only observed in cows	2260:2294	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	8	77	from	acid	1678:1681	arg1	cows					1697:1700	cows	1697:1700	cows	1697:1700	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	6	78	from	goats	1450:1454	arg1	increase					1427:1434	an increase	1424:1434	an increase of >C16 FA in goats, but not in cows	1424:1471	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	2	79	theme	Latin	896:900	arg1	design					909:914	a 4 × 4 Latin square design	888:914	a 4 × 4 Latin square design with 28-d experimental periods	888:945	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	12	80	from	trans-11	2629:2636	arg1	shift					2614:2618	a shift	2612:2618	a shift from the trans-11 toward the trans-10 ruminal pathways	2612:2673	This comparative study clearly demonstrated that each ruminant species responds differently to COS and HPO treatments, whereas MAP caused similar effects, and that goats are less sensitive than cows to diets that induce a shift from the trans-11 toward the trans-10 ruminal pathways.					
29885893	10	81	from	fold	2129:2132	arg1	average					2138:2144	average	2138:2144	average	2138:2144	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	8	82	theme	trans-10	1729:1736	arg1	18:1					1738:1741	trans-10 18:1	1729:1741	trans-10 18:1	1729:1741	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	2	83	theme	×	892:892	arg1	design					909:914	a 4 × 4 Latin square design	888:914	a 4 × 4 Latin square design with 28-d experimental periods	888:945	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	0	84	theme	milk	108:111	arg1	secretion					117:125	milk fat secretion	108:125	milk fat secretion	108:125	Diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil differentially modulate milk fat secretion and composition in cows and goats: A comparative study.					
29885893	2	85	theme	28-d	921:924	arg1	periods					939:945	28-d experimental periods	921:945	28-d experimental periods	921:945	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	1	86	theme	diets	336:340	arg1	types					327:331	2 types	325:331	2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown	325:502	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	8	87	from	fold	1708:1711	arg1	cows					1697:1700	cows	1697:1700	cows	1697:1700	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	10	88	from	decrease	2083:2090	arg1	concentration					2104:2116	the milk concentration	2095:2116	the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1	2095:2237	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	3	89	from	effects	986:992	arg1	yield					1002:1006	milk yield	997:1006	milk yield	997:1006	Dietary treatments had no significant effects on milk yield and DMI in both species, except for COS in cows, which decreased DMI by 17%.					
29885893	3	89	from	effects	986:992	arg1	DMI					1012:1014	DMI	1012:1014	DMI	1012:1014	Dietary treatments had no significant effects on milk yield and DMI in both species, except for COS in cows, which decreased DMI by 17%.					
29885893	3	89	from	effects	986:992	arg1	species					1024:1030	both species	1019:1030	both species	1019:1030	Dietary treatments had no significant effects on milk yield and DMI in both species, except for COS in cows, which decreased DMI by 17%.					
29885893	10	90	theme	18:0	2121:2124	arg1	concentration					2104:2116	the milk concentration	2095:2116	the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1	2095:2237	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	2	91	theme	marine	798:803	arg1	DMI					829:831	MAP; 1.5% DMI	819:831	MAP; 1.5% DMI	819:831	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	91	theme	marine	798:803	arg1	powder					811:816	marine algae powder	798:816	marine algae powder (MAP; 1.5% DMI)	798:832	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	1	92	from	effects	458:464	arg1	goats					469:473	goats	469:473	goats	469:473	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	3	93	theme	milk	997:1000	arg1	yield					1002:1006	milk yield	997:1006	milk yield	997:1006	Dietary treatments had no significant effects on milk yield and DMI in both species, except for COS in cows, which decreased DMI by 17%.					
29885893	1	94	theme	fat	359:361	arg1	depression					363:372	milk fat depression	354:372	milk fat depression in cows as well as a diet that increases milk fat content in cows	354:438	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	10	95	from	fold	2165:2168	arg1	average					2174:2180	average	2174:2180	average	2174:2180	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	2	96	theme	experimental	926:937	arg1	periods					939:945	28-d experimental periods	921:945	28-d experimental periods	921:945	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	1	97	theme	fatty	296:300	arg1	acid					302:305	milk fatty acid	291:305	milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown	291:502	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	1	97	theme	fatty	296:300	arg1	FA					308:309	FA	308:309	FA	308:309	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	9	98	theme	other	1793:1797	arg1	isomers					1824:1830	other conjugated linoleic acid isomers	1793:1830	other conjugated linoleic acid isomers	1793:1830	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	7	99	from	yield	1571:1575	arg1	cows					1590:1593	cows	1590:1593	cows	1590:1593	HPO supplementation increased the milk yield of C16 FA in cows.					
29885893	0	100	theme	palm	75:78	arg1	oil					80:82	hydrogenated palm oil	62:82	hydrogenated palm oil	62:82	Diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil differentially modulate milk fat secretion and composition in cows and goats: A comparative study.					
29885893	9	101	theme	linoleic	1810:1817	arg1	isomers					1824:1830	other conjugated linoleic acid isomers	1793:1830	other conjugated linoleic acid isomers	1793:1830	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	2	102	theme	wheat	778:782	arg1	COS					792:794	COS	792:794	COS	792:794	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	102	theme	wheat	778:782	arg1	starch					784:789	wheat starch	778:789	wheat starch (COS)	778:795	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	103	theme	Alpine	533:538	arg1	goats					540:544	12 Alpine goats	530:544	12 Alpine goats	530:544	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	104	theme	palm	851:854	arg1	oil					856:858	hydrogenated palm oil	838:858	hydrogenated palm oil (HPO; 3% DMI)	838:872	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	104	theme	palm	851:854	arg1	DMI					869:871	HPO; 3% DMI	861:871	HPO; 3% DMI	861:871	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	105	with	design	909:914	arg1	periods					939:945	28-d experimental periods	921:945	28-d experimental periods	921:945	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	8	106	from	cows	1757:1760	arg1	goats					1776:1780	goats	1776:1780	goats	1776:1780	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	2	107	theme	nonpregnant	564:574	arg1	DIM					607:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	107	theme	nonpregnant	564:574	arg1	cows					521:524	Twelve Holstein cows	505:524	Twelve Holstein cows	505:524	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	6	108	theme	>C16	1439:1442	arg1	FA					1444:1445	>C16 FA	1439:1445	>C16 FA in goats, but not in cows	1439:1471	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	12	109	theme	ruminant	2446:2453	arg1	species					2455:2461	each ruminant species	2441:2461	each ruminant species	2441:2461	This comparative study clearly demonstrated that each ruminant species responds differently to COS and HPO treatments, whereas MAP caused similar effects, and that goats are less sensitive than cows to diets that induce a shift from the trans-11 toward the trans-10 ruminal pathways.					
29885893	6	110	theme	FA	1487:1488	arg1	yield					1490:1494	the >C16 FA yield	1478:1494	the >C16 FA yield	1478:1494	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	9	111	from	goats	1964:1968	arg1	treatment					2010:2018	the COS treatment	2002:2018	the COS treatment	2002:2018	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	1	112	from	content	424:430	arg1	cows					435:438	cows	435:438	cows	435:438	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	2	113	theme	corn	736:739	arg1	DMI					768:770	DMI	768:770	DMI	768:770	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	113	theme	corn	736:739	arg1	intake					760:765	corn oil [5% dry matter intake	736:765	corn oil [5% dry matter intake (DMI)]	736:772	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	12	114	theme	trans-10	2649:2656	arg1	pathways					2666:2673	the trans-10 ruminal pathways	2645:2673	the trans-10 ruminal pathways	2645:2673	This comparative study clearly demonstrated that each ruminant species responds differently to COS and HPO treatments, whereas MAP caused similar effects, and that goats are less sensitive than cows to diets that induce a shift from the trans-11 toward the trans-10 ruminal pathways.					
29885893	2	115	theme	multiparous	551:561	arg1	DIM					607:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM	547:609	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	115	theme	multiparous	551:561	arg1	cows					521:524	Twelve Holstein cows	505:524	Twelve Holstein cows	505:524	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	12	116	theme	comparative	2397:2407	arg1	study					2409:2413	This comparative study	2392:2413	This comparative study	2392:2413	This comparative study clearly demonstrated that each ruminant species responds differently to COS and HPO treatments, whereas MAP caused similar effects, and that goats are less sensitive than cows to diets that induce a shift from the trans-11 toward the trans-10 ruminal pathways.					
29885893	9	117	theme	COS	2006:2008	arg1	treatment					2010:2018	the COS treatment	2002:2018	the COS treatment	2002:2018	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	4	118	theme	fat	1271:1273	arg1	content					1275:1281	milk fat content	1266:1281	milk fat content	1266:1281	In cows, milk fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (13%) compared with CTL, and in goats only MAP had an effect compared with CTL by decreasing milk fat content by 15%.					
29885893	5	119	theme	<C16	1364:1367	arg1	yields					1332:1337	the yields	1328:1337	the yields (mmol/d per kg of BW) of <C16 and C16 FA	1328:1378	In both species, COS and MAP lowered the yields (mmol/d per kg of BW) of <C16 and C16 FA.					
29885893	0	120	theme	corn	35:38	arg1	oil					40:42	corn oil	35:42	corn oil	35:42	Diets supplemented with starch and corn oil, marine algae, or hydrogenated palm oil differentially modulate milk fat secretion and composition in cows and goats: A comparative study.					
29885893	10	121	theme	18:1	2157:2160	arg1	concentration					2104:2116	the milk concentration	2095:2116	the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1	2095:2237	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	2	122	theme	dry	749:751	arg1	DMI					768:770	DMI	768:770	DMI	768:770	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	122	theme	dry	749:751	arg1	intake					760:765	corn oil [5% dry matter intake	736:765	corn oil [5% dry matter intake (DMI)]	736:772	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	5	123	theme	C16	1373:1375	arg1	FA					1377:1378	C16 FA	1373:1378	C16 FA	1373:1378	In both species, COS and MAP lowered the yields (mmol/d per kg of BW) of <C16 and C16 FA.					
29885893	4	124	theme	fat	1099:1101	arg1	content					1103:1109	milk fat content	1094:1109	milk fat content	1094:1109	In cows, milk fat content was lowered by COS (-45%) and MAP (-22%) and increased by HPO (13%) compared with CTL, and in goats only MAP had an effect compared with CTL by decreasing milk fat content by 15%.					
29885893	1	125	theme	animal	268:273	arg1	performance					275:285	the animal performance	264:285	the animal performance	264:285	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	8	126	theme	conjugated	1658:1667	arg1	acid					1678:1681	cis-12 conjugated linoleic acid	1651:1681	cis-12 conjugated linoleic acid by 18 fold in cows	1651:1700	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	2	127	theme	[5	745:746	arg1	DMI					768:770	DMI	768:770	DMI	768:770	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	127	theme	[5	745:746	arg1	intake					760:765	corn oil [5% dry matter intake	736:765	corn oil [5% dry matter intake (DMI)]	736:772	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	9	128	dep	cows	1916:1919	arg1	fold					1930:1933	8 and 4 fold	1922:1933	8 and 4 fold	1922:1933	Moreover, other conjugated linoleic acid isomers, such as trans-10,trans-12 and trans-7,cis-9, were increased to a greater extent in cows (8 and 4 fold, respectively) compared with goats (4 and 2 fold, respectively) on the COS treatment.					
29885893	1	129	theme	milk	415:418	arg1	content					424:430	milk fat content	415:430	milk fat content in cows	415:438	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	10	130	from	increase	2206:2213	arg1	18:1					2234:2237	the total trans 18:1	2218:2237	the total trans 18:1	2218:2237	In both species, the responses to MAP were characterized by a decrease in the milk concentration of 18:0 (3 fold, on average) and cis-9 18:1 (2 fold, on average) combined with a 3-fold increase in the total trans 18:1, with an increase in trans-10 18:1 only observed in cows.					
29885893	2	131	theme	%	827:827	arg1	DMI					829:831	MAP; 1.5% DMI	819:831	MAP; 1.5% DMI	819:831	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	2	131	theme	%	827:827	arg1	powder					811:816	marine algae powder	798:816	marine algae powder (MAP; 1.5% DMI)	798:832	Twelve Holstein cows and 12 Alpine goats, all multiparous, nonpregnant, and at 86 ± 24.9 and 61 ± 1.8 DIM, respectively, were allocated to 1 of 4 groups and fed diets containing no additional lipid (CTL) or diets supplemented with corn oil [5% dry matter intake (DMI)] and wheat starch (COS), marine algae powder (MAP; 1.5% DMI), or hydrogenated palm oil (HPO; 3% DMI), according to a 4 × 4 Latin square design with 28-d experimental periods.					
29885893	8	132	theme	acid	1678:1681	arg1	increase					1630:1637	an increase	1627:1637	an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1	1627:1741	Compared with CTL, COS induced an increase of trans-10,cis-12 conjugated linoleic acid by 18 fold in cows and 7 fold in goats and of trans-10 18:1 by 13 fold in cows and 3 fold in goats.					
29885893	6	133	from	cows	1468:1471	arg1	increase					1427:1434	an increase	1424:1434	an increase of >C16 FA in goats, but not in cows	1424:1471	With COS, this decrease was compensated by an increase of >C16 FA in goats, but not in cows, and the >C16 FA yield decreased with MAP in both species.					
29885893	1	134	theme	comparative	192:202	arg1	study					204:208	A direct comparative study	183:208	A direct comparative study of dairy cows and goats	183:232	A direct comparative study of dairy cows and goats was performed to characterize the animal performance and milk fatty acid (FA) responses to 2 types of diets that induce milk fat depression in cows as well as a diet that increases milk fat content in cows but for which the effects in goats are either absent or unknown.					
29885893	3	135	theme	Dietary	948:954	arg1	treatments					956:965	Dietary treatments	948:965	Dietary treatments	948:965	Dietary treatments had no significant effects on milk yield and DMI in both species, except for COS in cows, which decreased DMI by 17%.					
29885893	7	136	theme	C16	1580:1582	arg1	FA					1584:1585	C16 FA	1580:1585	C16 FA in cows	1580:1593	HPO supplementation increased the milk yield of C16 FA in cows.					
29589846	5	0	theme	tumor-bearing	794:806	arg1	mice					808:811	tumor-bearing mice	794:811	tumor-bearing mice	794:811	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	5	1	dep	in	768:769	arg1	vivo					771:774	vivo	771:774	vivo	771:774	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	1	2	theme	renal	162:166	arg1	nanoparticles					178:190	biocompatible and renal clearable nanoparticles	144:190	biocompatible and renal clearable nanoparticles	144:190	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	6	3	theme	X-ray	962:966	arg1	attenuation					968:978	strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals	955:1169	attenuation	968:978	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	1	4	theme	nanoparticles	263:275	arg1	composition					221:231	composition	221:231	composition	221:231	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	1	4	theme	nanoparticles	263:275	arg1	properties					245:254	surface properties	237:254	surface properties	237:254	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	1	4	theme	nanoparticles	263:275	arg1	diameter					211:218	diameter	211:218	diameter	211:218	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	1	5	theme	clearable	168:176	arg1	nanoparticles					178:190	biocompatible and renal clearable nanoparticles	144:190	biocompatible and renal clearable nanoparticles	144:190	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	6	6	dep	attenuation	968:978	arg1	ability					980:986	ability	980:986	ability	980:986	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	5	7	theme	In	711:712	arg1	cytotoxicity					720:731	In vitro cytotoxicity	711:731	In vitro cytotoxicity	711:731	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	3	8	theme	chemical	562:569	arg1	reduction					571:579	chemical reduction	562:579	chemical reduction	562:579	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	2	9	theme	templated	317:325	arg1	synthesis					327:335	cellulose nanofiber templated synthesis	297:335	cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles	297:372	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
29589846	5	10	theme	in	768:769	arg1	tests					783:787	in vivo animal tests	768:787	in vivo animal tests with tumor-bearing mice	768:811	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	4	11	theme	various	675:681	arg1	amounts					683:689	various amounts	675:689	various amounts of carboxyl groups	675:708	The sizes of nanoparticles are tailored in the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups.					
29589846	4	11	theme	various	675:681	arg1	groups					703:708	carboxyl groups	694:708	carboxyl groups	694:708	The sizes of nanoparticles are tailored in the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups.					
29589846	6	12	theme	X-ray	1064:1068	arg1	exposure					1070:1077	X-ray exposure	1064:1077	X-ray exposure	1064:1077	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	2	13	theme	nanofiber	307:315	arg1	synthesis					327:335	cellulose nanofiber templated synthesis	297:335	cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles	297:372	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
29589846	4	14	theme	nm	634:635	arg1	range					637:641	the 2-10 nm range	625:641	the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups	625:708	The sizes of nanoparticles are tailored in the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups.					
29589846	6	15	theme	strong	955:960	arg1	attenuation					968:978	strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals	955:1169	attenuation	968:978	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	3	16	theme	ions	511:514	arg1	spaces					444:449	The interstitial spaces	427:449	The interstitial spaces between adjacent fibers	427:473	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	3	16	theme	ions	511:514	arg1	sites					494:498	the adsorption sites	479:498	the adsorption sites of bismuth ions	479:514	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	3	16	theme	ions	511:514	arg1	ions					511:514	bismuth ions	503:514	bismuth ions	503:514	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	2	17	theme	cellulose	297:305	arg1	synthesis					327:335	cellulose nanofiber templated synthesis	297:335	cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles	297:372	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
29589846	5	18	theme	bismuth	905:911	arg1	nanoparticles					913:925	cellulose nanofiber-templated bismuth nanoparticles	875:925	cellulose nanofiber-templated bismuth nanoparticles	875:925	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	5	19	theme	animal	776:781	arg1	tests					783:787	in vivo animal tests	768:787	in vivo animal tests with tumor-bearing mice	768:811	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	4	20	theme	2-10	629:632	arg1	nm					634:635	nm	634:635	nm	634:635	The sizes of nanoparticles are tailored in the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups.					
29589846	3	21	theme	adsorption	483:492	arg1	spaces					444:449	The interstitial spaces	427:449	The interstitial spaces between adjacent fibers	427:473	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	3	21	theme	adsorption	483:492	arg1	sites					494:498	the adsorption sites	479:498	the adsorption sites of bismuth ions	479:514	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	3	21	theme	adsorption	483:492	arg1	ions					511:514	bismuth ions	503:514	bismuth ions	503:514	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	5	22	dep	In	711:712	arg1	vitro					714:718	vitro	714:718	vitro	714:718	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	2	23	theme	bismuth	352:358	arg1	nanoparticles					360:372	ultra-small bismuth nanoparticles	340:372	ultra-small bismuth nanoparticles	340:372	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
29589846	5	24	with	cytotoxicity	720:731	arg1	mice					808:811	tumor-bearing mice	794:811	tumor-bearing mice	794:811	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	2	25	theme	ultra-small	340:350	arg1	nanoparticles					360:372	ultra-small bismuth nanoparticles	340:372	ultra-small bismuth nanoparticles	340:372	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
29589846	2	26	theme	radiation	408:416	arg1	therapy					418:424	enhanced X-ray radiation therapy	393:424	enhanced X-ray radiation therapy	393:424	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
29589846	5	27	theme	X-ray	845:849	arg1	therapy					861:867	X-ray radiation therapy	845:867	X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles	845:925	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	4	28	theme	nanoparticles	595:607	arg1	sizes					586:590	The sizes	582:590	The sizes of nanoparticles	582:607	The sizes of nanoparticles are tailored in the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups.					
29589846	6	29	from	tumors	1153:1158	arg1	animals					1163:1169	animals	1163:1169	animals	1163:1169	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	2	30	dep	their	379:383	arg1	uses					385:388	uses	385:388	uses	385:388	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
29589846	6	31	theme	tumors	1153:1158	arg1	growth					1143:1148	growth	1143:1148	growth of tumors in animals	1143:1169	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	3	32	theme	bismuth	503:509	arg1	ions					511:514	bismuth ions	503:514	bismuth ions	503:514	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	1	33	theme	unmet	103:107	arg1	need					109:112	An unmet need	100:112	An unmet need in nanomedicine	100:128	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	4	34	theme	cellulose	649:657	arg1	nanofibers					659:668	cellulose nanofibers	649:668	cellulose nanofibers	649:668	The sizes of nanoparticles are tailored in the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups.					
29589846	6	35	theme	Bismuth	928:934	arg1	nanoparticles					936:948	Bismuth nanoparticles	928:948	Bismuth nanoparticles	928:948	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	1	36	theme	biocompatible	144:156	arg1	nanoparticles					178:190	biocompatible and renal clearable nanoparticles	144:190	biocompatible and renal clearable nanoparticles	144:190	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	0	37	theme	bismuth	46:52	arg1	nanoparticles					54:66	ultra-small bismuth nanoparticles	34:66	ultra-small bismuth nanoparticles	34:66	Nanocellulose templated growth of ultra-small bismuth nanoparticles for enhanced radiation therapy.					
29589846	5	38	theme	cellulose	875:883	arg1	nanoparticles					913:925	cellulose nanofiber-templated bismuth nanoparticles	875:925	cellulose nanofiber-templated bismuth nanoparticles	875:925	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	4	39	theme	carboxyl	694:701	arg1	groups					703:708	carboxyl groups	694:708	carboxyl groups	694:708	The sizes of nanoparticles are tailored in the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups.					
29589846	0	40	theme	ultra-small	34:44	arg1	nanoparticles					54:66	ultra-small bismuth nanoparticles	34:66	ultra-small bismuth nanoparticles	34:66	Nanocellulose templated growth of ultra-small bismuth nanoparticles for enhanced radiation therapy.					
29589846	6	41	theme	level	1035:1039	arg1	production					1041:1050	high level production	1030:1050	high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals	1030:1169	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	3	42	theme	adjacent	459:466	arg1	fibers					468:473	adjacent fibers	459:473	adjacent fibers	459:473	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	5	43	theme	nanofiber-templated	885:903	arg1	nanoparticles					913:925	cellulose nanofiber-templated bismuth nanoparticles	875:925	cellulose nanofiber-templated bismuth nanoparticles	875:925	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	3	44	theme	interstitial	431:442	arg1	spaces					444:449	The interstitial spaces	427:449	The interstitial spaces between adjacent fibers	427:473	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	3	44	theme	interstitial	431:442	arg1	sites					494:498	the adsorption sites	479:498	the adsorption sites of bismuth ions	479:514	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	3	44	theme	interstitial	431:442	arg1	ions					511:514	bismuth ions	503:514	bismuth ions	503:514	The interstitial spaces between adjacent fibers are the adsorption sites of bismuth ions and also stabilize nanoparticles generated by chemical reduction.					
29589846	6	45	theme	high	1030:1033	arg1	production					1041:1050	high level production	1030:1050	high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals	1030:1169	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	6	46	theme	ROS	1055:1057	arg1	cytotoxicity					1013:1024	concentration-dependent cytotoxicity	989:1024	concentration-dependent cytotoxicity	989:1024	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	6	46	theme	ROS	1055:1057	arg1	production					1041:1050	high level production	1030:1050	high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals	1030:1169	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	6	46	theme	ROS	1055:1057	arg1	attenuation					968:978	strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals	955:1169	attenuation	968:978	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	0	47	theme	nanoparticles	54:66	arg1	growth					24:29	growth	24:29	growth of ultra-small bismuth nanoparticles for enhanced radiation therapy	24:97	Nanocellulose templated growth of ultra-small bismuth nanoparticles for enhanced radiation therapy.					
29589846	1	48	from	need	109:112	arg1	nanomedicine					117:128	nanomedicine	117:128	nanomedicine	117:128	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	5	49	theme	reactive	734:741	arg1	ROS					759:761	ROS	759:761	ROS	759:761	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	5	49	theme	reactive	734:741	arg1	species					750:756	reactive oxygen species	734:756	reactive oxygen species (ROS)	734:762	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	6	50	from	growth	1143:1148	arg1	animals					1163:1169	animals	1163:1169	animals	1163:1169	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	1	51	theme	surface	237:243	arg1	properties					245:254	surface properties	237:254	surface properties	237:254	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	5	52	theme	oxygen	743:748	arg1	ROS					759:761	ROS	759:761	ROS	759:761	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	5	52	theme	oxygen	743:748	arg1	species					750:756	reactive oxygen species	734:756	reactive oxygen species (ROS)	734:762	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	2	53	theme	nanoparticles	360:372	arg1	synthesis					327:335	cellulose nanofiber templated synthesis	297:335	cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles	297:372	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
29589846	5	54	with	tests	783:787	arg1	mice					808:811	tumor-bearing mice	794:811	tumor-bearing mice	794:811	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	0	55	theme	radiation	81:89	arg1	therapy					91:97	enhanced radiation therapy	72:97	enhanced radiation therapy	72:97	Nanocellulose templated growth of ultra-small bismuth nanoparticles for enhanced radiation therapy.					
29589846	6	56	theme	cellular	1114:1121	arg1	damage					1123:1128	cellular damage	1114:1128	cellular damage	1114:1128	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	1	57	dep	diameter	211:218	arg1	the					207:209	the	207:209	the	207:209	An unmet need in nanomedicine is to prepare biocompatible and renal clearable nanoparticles by controlling the diameter, composition and surface properties of the nanoparticles.					
29589846	6	58	from	animals	1163:1169	arg1	growth					1143:1148	growth	1143:1148	growth of tumors in animals	1143:1169	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	2	59	theme	X-ray	402:406	arg1	therapy					418:424	enhanced X-ray radiation therapy	393:424	enhanced X-ray radiation therapy	393:424	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
29589846	4	60	theme	groups	703:708	arg1	amounts					683:689	various amounts	675:689	various amounts of carboxyl groups	675:708	The sizes of nanoparticles are tailored in the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups.					
29589846	4	60	theme	groups	703:708	arg1	groups					703:708	carboxyl groups	694:708	carboxyl groups	694:708	The sizes of nanoparticles are tailored in the 2-10 nm range using cellulose nanofibers with various amounts of carboxyl groups.					
29589846	0	61	theme	enhanced	72:79	arg1	therapy					91:97	enhanced radiation therapy	72:97	enhanced radiation therapy	72:97	Nanocellulose templated growth of ultra-small bismuth nanoparticles for enhanced radiation therapy.					
29589846	6	62	theme	concentration-dependent	989:1011	arg1	cytotoxicity					1013:1024	concentration-dependent cytotoxicity	989:1024	concentration-dependent cytotoxicity	989:1024	Bismuth nanoparticles show strong X-ray attenuation ability, concentration-dependent cytotoxicity and high level production of ROS upon X-ray exposure, which is consistent with enhanced cellular damage and retarded growth of tumors in animals.					
29589846	5	63	theme	radiation	851:859	arg1	therapy					861:867	X-ray radiation therapy	845:867	X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles	845:925	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	5	64	with	species	750:756	arg1	mice					808:811	tumor-bearing mice	794:811	tumor-bearing mice	794:811	In vitro cytotoxicity, reactive oxygen species (ROS) and in vivo animal tests with tumor-bearing mice are studied in order to enhance X-ray radiation therapy using cellulose nanofiber-templated bismuth nanoparticles.					
29589846	2	65	theme	enhanced	393:400	arg1	therapy					418:424	enhanced X-ray radiation therapy	393:424	enhanced X-ray radiation therapy	393:424	This paper reports cellulose nanofiber templated synthesis of ultra-small bismuth nanoparticles, and their uses in enhanced X-ray radiation therapy.					
30212678	1	0	theme	heavy	337:341	arg1	ions					349:352	heavy metal ions	337:352	heavy metal ions	337:352	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	1	1	theme	metal	343:347	arg1	ions					349:352	heavy metal ions	337:352	heavy metal ions	337:352	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	8	2	theme	Higuchi	1297:1303	arg1	models					1326:1331	Higuchi and Korsmeyer-Peppas models	1297:1331	Higuchi and Korsmeyer-Peppas models	1297:1331	The adsorption kinetics followed pseudo-second-order model, and the desorption process was fit well using Higuchi and Korsmeyer-Peppas models.					
30212678	8	3	theme	adsorption	1195:1204	arg1	kinetics					1206:1213	The adsorption kinetics	1191:1213	The adsorption kinetics	1191:1213	The adsorption kinetics followed pseudo-second-order model, and the desorption process was fit well using Higuchi and Korsmeyer-Peppas models.					
30212678	7	4	theme	MB	1133:1134	arg1	Pb2+					1140:1143	Pb2+	1140:1143	Pb2+	1140:1143	Moreover, around 5-8% and 20% of adsorbed MB and Pb2+ were released at the temperature above 45 °C.					
30212678	7	4	theme	MB	1133:1134	arg1	%					1111:1111	around 5-8%	1101:1111	around 5-8%	1101:1111	Moreover, around 5-8% and 20% of adsorbed MB and Pb2+ were released at the temperature above 45 °C.					
30212678	7	4	theme	MB	1133:1134	arg1	%					1119:1119	20%	1117:1119	20%	1117:1119	Moreover, around 5-8% and 20% of adsorbed MB and Pb2+ were released at the temperature above 45 °C.					
30212678	7	4	theme	MB	1133:1134	arg1	MB					1133:1134	adsorbed MB	1124:1134	adsorbed MB	1124:1134	Moreover, around 5-8% and 20% of adsorbed MB and Pb2+ were released at the temperature above 45 °C.					
30212678	0	5	from	Study	0:4	arg1	microfilaments					19:32	cellulose microfilaments	9:32	cellulose microfilaments	9:32	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	6	6	theme	adsorption	996:1005	arg1	equilibrium					1007:1017	the adsorption equilibrium	992:1017	the adsorption equilibrium reached within 80 min	992:1039	The results showed that P-MCCBs exhibited a fast adsorption rate, the adsorption equilibrium reached within 80 min and 40 min for MB and Pb2+, respectively (25 °C).					
30212678	2	7	theme	monomer	523:529	arg1	introduction					482:493	the introduction	478:493	the introduction of a temperature-responsive monomer (NIPAM)	478:537	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	2	7	theme	monomer	523:529	arg1	application					405:415	the application	401:415	the application of the nano-sized pore-forming agent (Calcium Carbonate)	401:472	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	3	8	theme	heating	700:706	arg1	approach					708:715	a microwave-assisted heating approach	679:715	a microwave-assisted heating approach	679:715	In addition, the spherical adsorbents were synthesized through an in-situ free radical polymerization using a microwave-assisted heating approach.					
30212678	5	9	dep	adsorption	826:835	arg1	The					822:824	The	822:824	The	822:824	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	4	10	theme	thermal	762:768	arg1	sensitivity					770:780	thermal sensitivity	762:780	thermal sensitivity	762:780	The morphology, chemical structure, pH, and thermal sensitivity of P-MCCBs were characterized properly.					
30212678	2	11	theme	temperature-responsive	500:521	arg1	NIPAM					532:536	NIPAM	532:536	NIPAM	532:536	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	2	11	theme	temperature-responsive	500:521	arg1	monomer					523:529	a temperature-responsive monomer	498:529	a temperature-responsive monomer (NIPAM)	498:537	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	8	12	theme	pseudo-second-order	1224:1242	arg1	model					1244:1248	pseudo-second-order model	1224:1248	pseudo-second-order model	1224:1248	The adsorption kinetics followed pseudo-second-order model, and the desorption process was fit well using Higuchi and Korsmeyer-Peppas models.					
30212678	4	13	theme	P-MCCBs	785:791	arg1	sensitivity					770:780	thermal sensitivity	762:780	thermal sensitivity	762:780	The morphology, chemical structure, pH, and thermal sensitivity of P-MCCBs were characterized properly.					
30212678	4	13	theme	P-MCCBs	785:791	arg1	pH					754:755	pH	754:755	pH	754:755	The morphology, chemical structure, pH, and thermal sensitivity of P-MCCBs were characterized properly.					
30212678	4	13	theme	P-MCCBs	785:791	arg1	morphology					722:731	morphology	722:731	morphology	722:731	The morphology, chemical structure, pH, and thermal sensitivity of P-MCCBs were characterized properly.					
30212678	4	13	theme	P-MCCBs	785:791	arg1	structure					743:751	chemical structure	734:751	chemical structure	734:751	The morphology, chemical structure, pH, and thermal sensitivity of P-MCCBs were characterized properly.					
30212678	2	14	theme	presented	374:382	arg1	work					384:387	the presented work	370:387	the presented work	370:387	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	5	15	theme	heavy	874:878	arg1	ions					886:889	heavy metal ions	874:889	heavy metal ions	874:889	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	1	16	theme	temperature-responsive	179:200	arg1	P-MCCBs					284:290	P-MCCBs	284:290	P-MCCBs	284:290	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	1	16	theme	temperature-responsive	179:200	arg1	spheres					275:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres	175:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs)	175:291	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	2	17	theme	agent	448:452	arg1	introduction					482:493	the introduction	478:493	the introduction of a temperature-responsive monomer (NIPAM)	478:537	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	2	17	theme	agent	448:452	arg1	application					405:415	the application	401:415	the application of the nano-sized pore-forming agent (Calcium Carbonate)	401:472	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	5	18	from	adsorption	826:835	arg1	P-MCCBs					894:900	P-MCCBs	894:900	P-MCCBs	894:900	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	6	19	dep	respectively	1069:1080	arg1	25 °C					1083:1087	25 °C	1083:1087	25 °C	1083:1087	The results showed that P-MCCBs exhibited a fast adsorption rate, the adsorption equilibrium reached within 80 min and 40 min for MB and Pb2+, respectively (25 °C).					
30212678	1	20	theme	polymer	202:208	arg1	P-MCCBs					284:290	P-MCCBs	284:290	P-MCCBs	284:290	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	1	20	theme	polymer	202:208	arg1	spheres					275:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres	175:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs)	175:291	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	2	21	theme	pore-forming	435:446	arg1	agent					448:452	the nano-sized pore-forming agent	420:452	the nano-sized pore-forming agent (Calcium Carbonate)	420:472	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	2	21	theme	pore-forming	435:446	arg1	Carbonate					463:471	Calcium Carbonate	455:471	Calcium Carbonate	455:471	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	5	22	theme	metal	880:884	arg1	ions					886:889	heavy metal ions	874:889	heavy metal ions	874:889	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	1	23	theme	grafted	210:216	arg1	P-MCCBs					284:290	P-MCCBs	284:290	P-MCCBs	284:290	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	1	23	theme	grafted	210:216	arg1	spheres					275:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres	175:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs)	175:291	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	2	24	theme	nano-sized	424:433	arg1	agent					448:452	the nano-sized pore-forming agent	420:452	the nano-sized pore-forming agent (Calcium Carbonate)	420:472	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	2	24	theme	nano-sized	424:433	arg1	Carbonate					463:471	Calcium Carbonate	455:471	Calcium Carbonate	455:471	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	9	25	theme	controllable	1411:1422	arg1	adsorption					1424:1433	controllable adsorption	1411:1433	controllable adsorption	1411:1433	These results indicated that P-MCCBs could be served as a novel material for controllable adsorption and desorption processes of various contaminants.					
30212678	1	26	theme	cellulose	218:226	arg1	P-MCCBs					284:290	P-MCCBs	284:290	P-MCCBs	284:290	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	1	26	theme	cellulose	218:226	arg1	spheres					275:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres	175:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs)	175:291	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	0	27	theme	cellulose	9:17	arg1	microfilaments					19:32	cellulose microfilaments	9:32	cellulose microfilaments	9:32	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	6	28	theme	adsorption	975:984	arg1	rate					986:989	a fast adsorption rate	968:989	a fast adsorption rate	968:989	The results showed that P-MCCBs exhibited a fast adsorption rate, the adsorption equilibrium reached within 80 min and 40 min for MB and Pb2+, respectively (25 °C).					
30212678	0	29	theme	pollutant	126:134	arg1	removal					136:142	pollutant removal	126:142	pollutant removal	126:142	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	3	30	theme	free	645:648	arg1	polymerization					658:671	an in-situ free radical polymerization	634:671	an in-situ free radical polymerization using a microwave-assisted heating approach	634:715	In addition, the spherical adsorbents were synthesized through an in-situ free radical polymerization using a microwave-assisted heating approach.					
30212678	4	31	dep	morphology	722:731	arg1	The					718:720	The	718:720	The	718:720	The morphology, chemical structure, pH, and thermal sensitivity of P-MCCBs were characterized properly.					
30212678	1	32	theme	filaments/Poly	228:241	arg1	P-MCCBs					284:290	P-MCCBs	284:290	P-MCCBs	284:290	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	1	32	theme	filaments/Poly	228:241	arg1	spheres					275:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres	175:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs)	175:291	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	0	33	theme	composite	40:48	arg1	application					111:121	application	111:121	application	111:121	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	0	33	theme	composite	40:48	arg1	characterization					89:104	characterization	89:104	characterization	89:104	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	0	33	theme	composite	40:48	arg1	spheres					50:56	composite spheres	40:56	composite spheres: Microwave-assisted synthesis, characterization, and application	40:121	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	0	33	theme	composite	40:48	arg1	synthesis					78:86	Microwave-assisted synthesis	59:86	Microwave-assisted synthesis	59:86	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	6	34	theme	fast	970:973	arg1	rate					986:989	a fast adsorption rate	968:989	a fast adsorption rate	968:989	The results showed that P-MCCBs exhibited a fast adsorption rate, the adsorption equilibrium reached within 80 min and 40 min for MB and Pb2+, respectively (25 °C).					
30212678	3	35	theme	radical	650:656	arg1	polymerization					658:671	an in-situ free radical polymerization	634:671	an in-situ free radical polymerization using a microwave-assisted heating approach	634:715	In addition, the spherical adsorbents were synthesized through an in-situ free radical polymerization using a microwave-assisted heating approach.					
30212678	9	36	theme	desorption	1439:1448	arg1	processes					1450:1458	desorption processes	1439:1458	desorption processes	1439:1458	These results indicated that P-MCCBs could be served as a novel material for controllable adsorption and desorption processes of various contaminants.					
30212678	5	37	theme	dyes	865:868	arg1	adsorption					826:835	adsorption	826:835	adsorption	826:835	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	5	37	theme	dyes	865:868	arg1	behaviors					852:860	desorption behaviors	841:860	desorption behaviors	841:860	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	7	38	theme	adsorbed	1124:1131	arg1	MB					1133:1134	adsorbed MB	1124:1134	adsorbed MB	1124:1134	Moreover, around 5-8% and 20% of adsorbed MB and Pb2+ were released at the temperature above 45 °C.					
30212678	5	39	theme	ions	886:889	arg1	adsorption					826:835	adsorption	826:835	adsorption	826:835	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	5	39	theme	ions	886:889	arg1	behaviors					852:860	desorption behaviors	841:860	desorption behaviors	841:860	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	0	40	dep	spheres	50:56	arg1	spheres					50:56	composite spheres	40:56	composite spheres: Microwave-assisted synthesis, characterization, and application	40:121	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	0	40	dep	spheres	50:56	arg1	characterization					89:104	characterization	89:104	characterization	89:104	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	0	40	dep	spheres	50:56	arg1	application					111:121	application	111:121	application	111:121	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	0	40	dep	spheres	50:56	arg1	synthesis					78:86	Microwave-assisted synthesis	59:86	Microwave-assisted synthesis	59:86	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	1	41	theme	N-isopropylacrylamide	244:264	arg1	P-MCCBs					284:290	P-MCCBs	284:290	P-MCCBs	284:290	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	1	41	theme	N-isopropylacrylamide	244:264	arg1	spheres					275:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres	175:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs)	175:291	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	2	42	theme	Calcium	455:461	arg1	agent					448:452	the nano-sized pore-forming agent	420:452	the nano-sized pore-forming agent (Calcium Carbonate)	420:472	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	2	42	theme	Calcium	455:461	arg1	Carbonate					463:471	Calcium Carbonate	455:471	Calcium Carbonate	455:471	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	3	43	theme	in-situ	637:643	arg1	polymerization					658:671	an in-situ free radical polymerization	634:671	an in-situ free radical polymerization using a microwave-assisted heating approach	634:715	In addition, the spherical adsorbents were synthesized through an in-situ free radical polymerization using a microwave-assisted heating approach.					
30212678	9	44	theme	various	1463:1469	arg1	contaminants					1471:1482	various contaminants	1463:1482	various contaminants	1463:1482	These results indicated that P-MCCBs could be served as a novel material for controllable adsorption and desorption processes of various contaminants.					
30212678	1	45	theme	ions	349:352	arg1	removal					317:323	the removal	313:323	the removal of dyes and heavy metal ions	313:352	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	5	46	from	behaviors	852:860	arg1	P-MCCBs					894:900	P-MCCBs	894:900	P-MCCBs	894:900	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	8	47	theme	desorption	1259:1268	arg1	process					1270:1276	the desorption process	1255:1276	the desorption process	1255:1276	The adsorption kinetics followed pseudo-second-order model, and the desorption process was fit well using Higuchi and Korsmeyer-Peppas models.					
30212678	1	48	theme	dyes	328:331	arg1	removal					317:323	the removal	313:323	the removal of dyes and heavy metal ions	313:352	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	3	49	theme	microwave-assisted	681:698	arg1	approach					708:715	a microwave-assisted heating approach	679:715	a microwave-assisted heating approach	679:715	In addition, the spherical adsorbents were synthesized through an in-situ free radical polymerization using a microwave-assisted heating approach.					
30212678	3	50	theme	spherical	588:596	arg1	adsorbents					598:607	the spherical adsorbents	584:607	the spherical adsorbents	584:607	In addition, the spherical adsorbents were synthesized through an in-situ free radical polymerization using a microwave-assisted heating approach.					
30212678	9	51	theme	novel	1392:1396	arg1	material					1398:1405	a novel material	1390:1405	a novel material for controllable adsorption and desorption processes of various contaminants	1390:1482	These results indicated that P-MCCBs could be served as a novel material for controllable adsorption and desorption processes of various contaminants.					
30212678	4	52	theme	chemical	734:741	arg1	structure					743:751	chemical structure	734:751	chemical structure	734:751	The morphology, chemical structure, pH, and thermal sensitivity of P-MCCBs were characterized properly.					
30212678	8	53	theme	Korsmeyer-Peppas	1309:1324	arg1	models					1326:1331	Higuchi and Korsmeyer-Peppas models	1297:1331	Higuchi and Korsmeyer-Peppas models	1297:1331	The adsorption kinetics followed pseudo-second-order model, and the desorption process was fit well using Higuchi and Korsmeyer-Peppas models.					
30212678	9	54	theme	contaminants	1471:1482	arg1	adsorption					1424:1433	controllable adsorption	1411:1433	controllable adsorption	1411:1433	These results indicated that P-MCCBs could be served as a novel material for controllable adsorption and desorption processes of various contaminants.					
30212678	9	54	theme	contaminants	1471:1482	arg1	processes					1450:1458	desorption processes	1439:1458	desorption processes	1439:1458	These results indicated that P-MCCBs could be served as a novel material for controllable adsorption and desorption processes of various contaminants.					
30212678	1	55	theme	novel	147:151	arg1	study					153:157	A novel study	145:157	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs)	145:291	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	2	56	theme	work	384:387	arg1	novelty					359:365	The novelty	355:365	The novelty of the presented work	355:387	The novelty of the presented work consists of the application of the nano-sized pore-forming agent (Calcium Carbonate) and the introduction of a temperature-responsive monomer (NIPAM) while preparing the adsorbents.					
30212678	1	57	theme	NIPAM	268:272	arg1	P-MCCBs					284:290	P-MCCBs	284:290	P-MCCBs	284:290	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	1	57	theme	NIPAM	268:272	arg1	spheres					275:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres	175:281	the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs)	175:291	A novel study of synthesizing the temperature-responsive polymer grafted cellulose filaments/Poly (N-isopropylacrylamide) (NIPAM) spheres (P-MCCBs) was carried out for the removal of dyes and heavy metal ions.					
30212678	0	58	theme	Microwave-assisted	59:76	arg1	spheres					50:56	composite spheres	40:56	composite spheres: Microwave-assisted synthesis, characterization, and application	40:121	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	0	58	theme	Microwave-assisted	59:76	arg1	synthesis					78:86	Microwave-assisted synthesis	59:86	Microwave-assisted synthesis	59:86	Study on cellulose microfilaments based composite spheres: Microwave-assisted synthesis, characterization, and application in pollutant removal.					
30212678	5	59	theme	desorption	841:850	arg1	behaviors					852:860	desorption behaviors	841:860	desorption behaviors	841:860	The adsorption and desorption behaviors of dyes and heavy metal ions on P-MCCBs were also investigated.					
30212678	7	60	theme	Pb2+	1140:1143	arg1	Pb2+					1140:1143	Pb2+	1140:1143	Pb2+	1140:1143	Moreover, around 5-8% and 20% of adsorbed MB and Pb2+ were released at the temperature above 45 °C.					
30212678	7	60	theme	Pb2+	1140:1143	arg1	%					1111:1111	around 5-8%	1101:1111	around 5-8%	1101:1111	Moreover, around 5-8% and 20% of adsorbed MB and Pb2+ were released at the temperature above 45 °C.					
30212678	7	60	theme	Pb2+	1140:1143	arg1	%					1119:1119	20%	1117:1119	20%	1117:1119	Moreover, around 5-8% and 20% of adsorbed MB and Pb2+ were released at the temperature above 45 °C.					
30212678	7	60	theme	Pb2+	1140:1143	arg1	MB					1133:1134	adsorbed MB	1124:1134	adsorbed MB	1124:1134	Moreover, around 5-8% and 20% of adsorbed MB and Pb2+ were released at the temperature above 45 °C.					
30500499	7	0	from	4 °C	1039:1042	arg1	laccase					1023:1029	laccase	1023:1029	laccase at both 4 °C and 25 °C	1023:1052	Moreover, the storage stability of laccase at both 4 °C and 25 °C was increased after immobilization.					
30500499	7	0	from	4 °C	1039:1042	arg1	stability					1010:1018	the storage stability	998:1018	the storage stability of laccase at both 4 °C and 25 °C	998:1052	Moreover, the storage stability of laccase at both 4 °C and 25 °C was increased after immobilization.					
30500499	4	1	theme	apparent	571:578	arg1	activity					580:587	3.5 Units/g apparent activity	559:587	3.5 Units/g apparent activity	559:587	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	6	2	theme	encapsulated	854:865	arg1	laccase					867:873	The encapsulated laccase	850:873	The encapsulated laccase	850:873	The encapsulated laccase showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme.					
30500499	10	3	theme	different	1468:1476	arg1	applications					1478:1489	different applications	1468:1489	different applications	1468:1489	Thus, the encapsulation of the laccase is an efficient method to keep the enzyme active and stable for different applications.					
30500499	3	4	theme	chitosan-nanobiochar	371:390	arg1	matrix					392:397	a chitosan-nanobiochar matrix	369:397	a chitosan-nanobiochar matrix	369:397	In this study, laccase enzyme from Trametes versicolor was encapsulated for the first time in a chitosan-nanobiochar matrix.					
30500499	4	5	theme	Units/g	563:569	arg1	activity					580:587	3.5 Units/g apparent activity	559:587	3.5 Units/g apparent activity	559:587	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	4	6	theme	formation	434:442	arg1	technique					444:452	The chitosan-tripolyphosphate gel formation technique	400:452	The chitosan-tripolyphosphate gel formation technique	400:452	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	7	7	theme	storage	1002:1008	arg1	stability					1010:1018	the storage stability	998:1018	the storage stability of laccase at both 4 °C and 25 °C	998:1052	Moreover, the storage stability of laccase at both 4 °C and 25 °C was increased after immobilization.					
30500499	9	8	theme	encapsulated	1286:1297	arg1	laccase					1299:1305	encapsulated laccase	1286:1305	encapsulated laccase	1286:1305	Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones.					
30500499	4	9	theme	effective	526:534	arg1	efficiency					544:553	35% effective binding efficiency	522:553	35% effective binding efficiency	522:553	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	8	10	theme	laccase	1101:1107	arg1	laccase					1101:1107	laccase	1101:1107	laccase	1101:1107	Only 2% of laccase was leaked during a 5-day period from biocatalyst.					
30500499	8	10	theme	laccase	1101:1107	arg1	%					1096:1096	Only 2%	1090:1096	Only 2% of laccase	1090:1107	Only 2% of laccase was leaked during a 5-day period from biocatalyst.					
30500499	6	11	theme	moderate	884:891	arg1	increase					893:900	a moderate increase	882:900	a moderate increase in enzyme stability against pH and temperature variation	882:957	The encapsulated laccase showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme.					
30500499	4	12	theme	%	524:524	arg1	efficiency					544:553	35% effective binding efficiency	522:553	35% effective binding efficiency	522:553	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	10	13	theme	stable	1457:1462	arg1	enzyme					1439:1444	the enzyme	1435:1444	the enzyme active and stable	1435:1462	Thus, the encapsulation of the laccase is an efficient method to keep the enzyme active and stable for different applications.					
30500499	7	14	from	25 °C	1048:1052	arg1	laccase					1023:1029	laccase	1023:1029	laccase at both 4 °C and 25 °C	1023:1052	Moreover, the storage stability of laccase at both 4 °C and 25 °C was increased after immobilization.					
30500499	7	14	from	25 °C	1048:1052	arg1	stability					1010:1018	the storage stability	998:1018	the storage stability of laccase at both 4 °C and 25 °C	998:1052	Moreover, the storage stability of laccase at both 4 °C and 25 °C was increased after immobilization.					
30500499	5	15	theme	initial	817:823	arg1	activity					825:832	the initial activity	813:832	the initial activity after 5 cycles	813:847	The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles.					
30500499	8	16	from	biocatalyst	1147:1157	arg1	period					1135:1140	a 5-day period	1127:1140	a 5-day period from biocatalyst	1127:1157	Only 2% of laccase was leaked during a 5-day period from biocatalyst.					
30500499	6	17	dep	showed	875:880	arg1	compared					959:966	compared	959:966	showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme	875:985	The encapsulated laccase showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme.					
30500499	4	18	theme	binding	536:542	arg1	efficiency					544:553	35% effective binding efficiency	522:553	35% effective binding efficiency	522:553	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	5	19	theme	2,2'-azinobis-	705:718	arg1	oxidation					692:700	the oxidation	688:700	the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles	688:847	The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles.					
30500499	5	20	theme	activity	825:832	arg1	%					808:808	30%	806:808	30% of the initial activity after 5 cycles	806:847	The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles.					
30500499	5	20	theme	activity	825:832	arg1	activity					825:832	the initial activity	813:832	the initial activity after 5 cycles	813:847	The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles.					
30500499	6	21	theme	free	975:978	arg1	enzyme					980:985	the free enzyme	971:985	the free enzyme	971:985	The encapsulated laccase showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme.					
30500499	2	22	theme	accepted	212:219	arg1	approach					221:228	a universally accepted approach	198:228	a universally accepted approach to increase their reusability and stability	198:272	Immobilization of enzymes is a universally accepted approach to increase their reusability and stability.					
30500499	2	22	theme	accepted	212:219	arg1	Immobilization					169:182	Immobilization	169:182	Immobilization of enzymes	169:193	Immobilization of enzymes is a universally accepted approach to increase their reusability and stability.					
30500499	4	23	theme	gel	430:432	arg1	technique					444:452	The chitosan-tripolyphosphate gel formation technique	400:452	The chitosan-tripolyphosphate gel formation technique	400:452	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	4	24	with	nanoparticles	502:514	arg1	activity					580:587	3.5 Units/g apparent activity	559:587	3.5 Units/g apparent activity	559:587	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	4	24	with	nanoparticles	502:514	arg1	efficiency					544:553	35% effective binding efficiency	522:553	35% effective binding efficiency	522:553	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	0	25	theme	using	31:35	arg1	nanobiocatalyst					15:29	nanobiocatalyst using	15:35	nanobiocatalyst using	15:35	Fabrication of nanobiocatalyst using encapsulated laccase onto chitosan-nanobiochar composite.					
30500499	5	26	theme	encapsulated	642:653	arg1	laccase					655:661	the encapsulated laccase	638:661	the encapsulated laccase	638:661	The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles.					
30500499	4	27	theme	chitosan-tripolyphosphate	404:428	arg1	technique					444:452	The chitosan-tripolyphosphate gel formation technique	400:452	The chitosan-tripolyphosphate gel formation technique	400:452	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	9	28	theme	Gram-negative	1244:1256	arg1	microorganisms					1264:1277	Gram positive and Gram-negative model microorganisms	1226:1277	microorganisms	1264:1277	Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones.					
30500499	0	29	theme	nanobiocatalyst	15:29	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of nanobiocatalyst using	0:35	Fabrication of nanobiocatalyst using encapsulated laccase onto chitosan-nanobiochar composite.					
30500499	6	30	dep	pH	930:931	arg1	variation					949:957	variation	949:957	variation	949:957	The encapsulated laccase showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme.					
30500499	9	31	theme	free	1175:1178	arg1	form					1180:1183	its free form	1171:1183	its free form	1171:1183	Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones.					
30500499	10	32	theme	laccase	1396:1402	arg1	encapsulation					1375:1387	the encapsulation	1371:1387	the encapsulation of the laccase	1371:1402	Thus, the encapsulation of the laccase is an efficient method to keep the enzyme active and stable for different applications.					
30500499	10	32	theme	laccase	1396:1402	arg1	method					1420:1425	an efficient method	1407:1425	an efficient method to keep the enzyme active and stable for different applications	1407:1489	Thus, the encapsulation of the laccase is an efficient method to keep the enzyme active and stable for different applications.					
30500499	7	33	from	stability	1010:1018	arg1	25 °C					1048:1052	25 °C	1048:1052	25 °C	1048:1052	Moreover, the storage stability of laccase at both 4 °C and 25 °C was increased after immobilization.					
30500499	7	33	from	stability	1010:1018	arg1	4 °C					1039:1042	4 °C	1039:1042	4 °C	1039:1042	Moreover, the storage stability of laccase at both 4 °C and 25 °C was increased after immobilization.					
30500499	9	34	theme	antibacterial	1314:1326	arg1	activity					1328:1335	antibacterial activity	1314:1335	antibacterial activity towards Gram-positive ones	1314:1362	Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones.					
30500499	6	35	from	increase	893:900	arg1	stability					912:920	enzyme stability	905:920	enzyme stability against pH and temperature variation	905:957	The encapsulated laccase showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme.					
30500499	3	36	theme	laccase	290:296	arg1	enzyme					298:303	laccase enzyme	290:303	laccase enzyme from Trametes versicolor	290:328	In this study, laccase enzyme from Trametes versicolor was encapsulated for the first time in a chitosan-nanobiochar matrix.					
30500499	4	37	theme	35	522:523	arg1	%					524:524	%	524:524	%	524:524	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	0	38	theme	chitosan-nanobiochar	63:82	arg1	composite					84:92	chitosan-nanobiochar composite	63:92	chitosan-nanobiochar composite	63:92	Fabrication of nanobiocatalyst using encapsulated laccase onto chitosan-nanobiochar composite.					
30500499	9	39	theme	model	1258:1262	arg1	microorganisms					1264:1277	Gram positive and Gram-negative model microorganisms	1226:1277	microorganisms	1264:1277	Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones.					
30500499	2	40	theme	enzymes	187:193	arg1	approach					221:228	a universally accepted approach	198:228	a universally accepted approach to increase their reusability and stability	198:272	Immobilization of enzymes is a universally accepted approach to increase their reusability and stability.					
30500499	2	40	theme	enzymes	187:193	arg1	Immobilization					169:182	Immobilization	169:182	Immobilization of enzymes	169:193	Immobilization of enzymes is a universally accepted approach to increase their reusability and stability.					
30500499	9	41	from	Laccase	1160:1166	arg1	form					1180:1183	its free form	1171:1183	its free form	1171:1183	Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones.					
30500499	5	42	theme	several	767:773	arg1	cycles					787:792	several consecutive cycles	767:792	several consecutive cycles	767:792	The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles.					
30500499	1	43	theme	used	124:127	arg1	enzymes					129:135	the widely used enzymes	113:135	the widely used enzymes for biotechnological processes	113:166	Laccase is one of the widely used enzymes for biotechnological processes.					
30500499	10	44	theme	efficient	1410:1418	arg1	encapsulation					1375:1387	the encapsulation	1371:1387	the encapsulation of the laccase	1371:1402	Thus, the encapsulation of the laccase is an efficient method to keep the enzyme active and stable for different applications.					
30500499	10	44	theme	efficient	1410:1418	arg1	method					1420:1425	an efficient method	1407:1425	an efficient method to keep the enzyme active and stable for different applications	1407:1489	Thus, the encapsulation of the laccase is an efficient method to keep the enzyme active and stable for different applications.					
30500499	4	45	theme	best	599:602	arg1	configuration					604:616	the best configuration	595:616	the best configuration	595:616	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	9	46	theme	Gram-positive	1345:1357	arg1	ones					1359:1362	Gram-positive ones	1345:1362	Gram-positive ones	1345:1362	Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones.					
30500499	1	47	theme	enzymes	129:135	arg1	enzymes					129:135	the widely used enzymes	113:135	the widely used enzymes for biotechnological processes	113:166	Laccase is one of the widely used enzymes for biotechnological processes.					
30500499	1	47	theme	enzymes	129:135	arg1	one					106:108	one	106:108	one	106:108	Laccase is one of the widely used enzymes for biotechnological processes.					
30500499	5	48	theme	laccase	655:661	arg1	reusability					623:633	The reusability	619:633	The reusability of the encapsulated laccase	619:661	The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles.					
30500499	3	49	theme	Trametes	310:317	arg1	versicolor					319:328	Trametes versicolor	310:328	Trametes versicolor	310:328	In this study, laccase enzyme from Trametes versicolor was encapsulated for the first time in a chitosan-nanobiochar matrix.					
30500499	9	50	theme	antibacterial	1195:1207	arg1	activity					1209:1216	no antibacterial activity	1192:1216	no antibacterial activity against Gram positive and Gram-negative model microorganisms	1192:1277	Laccase in its free form showed no antibacterial activity against Gram positive and Gram-negative model microorganisms, while encapsulated laccase showed antibacterial activity towards Gram-positive ones.					
30500499	5	51	theme	consecutive	775:785	arg1	cycles					787:792	several consecutive cycles	767:792	several consecutive cycles	767:792	The reusability of the encapsulated laccase was demonstrated towards the oxidation of 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) for several consecutive cycles, exhibiting 30% of the initial activity after 5 cycles.					
30500499	4	52	theme	biocatalyst	490:500	arg1	nanoparticles					502:514	homogeneous biocatalyst nanoparticles	478:514	homogeneous biocatalyst nanoparticles	478:514	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	1	53	theme	biotechnological	141:156	arg1	processes					158:166	biotechnological processes	141:166	biotechnological processes	141:166	Laccase is one of the widely used enzymes for biotechnological processes.					
30500499	3	54	from	versicolor	319:328	arg1	enzyme					298:303	laccase enzyme	290:303	laccase enzyme from Trametes versicolor	290:328	In this study, laccase enzyme from Trametes versicolor was encapsulated for the first time in a chitosan-nanobiochar matrix.					
30500499	3	55	theme	first	355:359	arg1	time					361:364	the first time	351:364	the first time	351:364	In this study, laccase enzyme from Trametes versicolor was encapsulated for the first time in a chitosan-nanobiochar matrix.					
30500499	4	56	theme	homogeneous	478:488	arg1	nanoparticles					502:514	homogeneous biocatalyst nanoparticles	478:514	homogeneous biocatalyst nanoparticles	478:514	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	10	57	theme	active	1446:1451	arg1	enzyme					1439:1444	the enzyme	1435:1444	the enzyme active and stable	1435:1462	Thus, the encapsulation of the laccase is an efficient method to keep the enzyme active and stable for different applications.					
30500499	6	58	theme	enzyme	905:910	arg1	stability					912:920	enzyme stability	905:920	enzyme stability against pH and temperature variation	905:957	The encapsulated laccase showed a moderate increase in enzyme stability against pH and temperature variation compared to the free enzyme.					
30500499	7	59	theme	laccase	1023:1029	arg1	stability					1010:1018	the storage stability	998:1018	the storage stability of laccase at both 4 °C and 25 °C	998:1052	Moreover, the storage stability of laccase at both 4 °C and 25 °C was increased after immobilization.					
30500499	4	60	theme	3.5	559:561	arg1	Units/g					563:569	Units/g	563:569	Units/g	563:569	The chitosan-tripolyphosphate gel formation technique was employed to produce homogeneous biocatalyst nanoparticles, with 35% effective binding efficiency and 3.5 Units/g apparent activity under the best configuration.					
30500499	8	61	theme	5-day	1129:1133	arg1	period					1135:1140	a 5-day period	1127:1140	a 5-day period from biocatalyst	1127:1157	Only 2% of laccase was leaked during a 5-day period from biocatalyst.					
31505895	9	0	theme	different	1370:1378	arg1	conditions					1380:1389	different conditions	1370:1389	different conditions	1370:1389	Furthermore, the in-feed stabilities of the probiotic strains were evaluated under different conditions.					
31505895	3	1	link	broiler-derived	468:482	arg1	lactobacilli					494:505	two broiler-derived probiotic lactobacilli	464:505	two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis)	464:535	In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis).					
31505895	11	2	theme	promising	1649:1657	arg1	results					1659:1665	promising results	1649:1665	promising results	1649:1665	Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.					
31505895	4	3	from	production	640:649	arg1	fermentations					664:676	lab-scale fermentations	654:676	lab-scale fermentations	654:676	Carbohydrate utilization of both strains was determined and preferred substrates that boosted biomass production in lab-scale fermentations were selected.					
31505895	4	4	theme	Carbohydrate	538:549	arg1	utilization					551:561	Carbohydrate utilization	538:561	Carbohydrate utilization of both strains	538:577	Carbohydrate utilization of both strains was determined and preferred substrates that boosted biomass production in lab-scale fermentations were selected.					
31505895	8	5	dep	trehalose	1181:1189	arg1	salivarius					1195:1204	L. salivarius	1192:1204	L. salivarius	1192:1204	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	1	6	theme	antibiotic	168:177	arg1	promoters					186:194	antibiotic growth promoters	168:194	antibiotic growth promoters	168:194	In animal nutrition, probiotics are considered as desirable alternatives to antibiotic growth promoters.					
31505895	5	7	theme	good	712:715	arg1	tolerance					725:733	good aerobic tolerance	712:733	good aerobic tolerance	712:733	The strains showed good aerobic tolerance, which resulted in easier scale-up production.					
31505895	4	8	theme	biomass	632:638	arg1	production					640:649	biomass production	632:649	biomass production in lab-scale fermentations	632:676	Carbohydrate utilization of both strains was determined and preferred substrates that boosted biomass production in lab-scale fermentations were selected.					
31505895	5	9	theme	aerobic	717:723	arg1	tolerance					725:733	good aerobic tolerance	712:733	good aerobic tolerance	712:733	The strains showed good aerobic tolerance, which resulted in easier scale-up production.					
31505895	7	10	dep	factors	976:982	arg1	factors					976:982	three protective factors	959:982	three protective factors (skim milk, sucrose, and trehalose)	959:1018	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	7	10	dep	factors	976:982	arg1	sucrose					996:1002	sucrose	996:1002	sucrose	996:1002	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	7	10	dep	factors	976:982	arg1	trehalose					1009:1017	trehalose	1009:1017	trehalose	1009:1017	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	7	10	dep	factors	976:982	arg1	milk					990:993	skim milk	985:993	skim milk	985:993	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	2	11	theme	technical	293:301	arg1	optimization					303:314	technical optimization	293:314	technical optimization of biomass production	293:336	The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific.					
31505895	7	12	theme	polynomial	923:932	arg1	model					934:938	A quadratic polynomial model	911:938	A quadratic polynomial model	911:938	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	8	13	theme	0.15g/mL	1211:1218	arg1	g/mL					1255:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL	1211:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis)	1211:1270	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	13	theme	0.15g/mL	1211:1218	arg1	agilis					1264:1269	L. agilis	1261:1269	L. agilis	1261:1269	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	9	14	theme	probiotic	1331:1339	arg1	strains					1341:1347	the probiotic strains	1327:1347	the probiotic strains	1327:1347	Furthermore, the in-feed stabilities of the probiotic strains were evaluated under different conditions.					
31505895	11	15	theme	maximum	1562:1568	arg1	stability					1570:1578	the maximum stability	1558:1578	the maximum stability of both Lactobacillus strains	1558:1608	Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.					
31505895	11	16	from	°C	1546:1547	arg1	storage					1518:1524	only storage	1513:1524	only storage of the strains at 4 °C	1513:1547	Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.					
31505895	1	17	theme	growth	179:184	arg1	promoters					186:194	antibiotic growth promoters	168:194	antibiotic growth promoters	168:194	In animal nutrition, probiotics are considered as desirable alternatives to antibiotic growth promoters.					
31505895	9	18	theme	strains	1341:1347	arg1	stabilities					1312:1322	the in-feed stabilities	1300:1322	the in-feed stabilities of the probiotic strains	1300:1347	Furthermore, the in-feed stabilities of the probiotic strains were evaluated under different conditions.					
31505895	8	19	theme	optimal	1093:1099	arg1	combination					1101:1111	The optimal combination	1089:1111	The optimal combination of protectants	1089:1126	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	19	theme	optimal	1093:1099	arg1	trehalose					1181:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose	1132:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius)	1132:1205	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	11	20	theme	strains	1602:1608	arg1	stability					1570:1578	the maximum stability	1558:1578	the maximum stability of both Lactobacillus strains	1558:1608	Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.					
31505895	8	21	theme	skim	1220:1223	arg1	g/mL					1255:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL	1211:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis)	1211:1270	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	21	theme	skim	1220:1223	arg1	agilis					1264:1269	L. agilis	1261:1269	L. agilis	1261:1269	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	22	theme	L.	1261:1262	arg1	g/mL					1255:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL	1211:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis)	1211:1270	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	22	theme	L.	1261:1262	arg1	agilis					1264:1269	L. agilis	1261:1269	L. agilis	1261:1269	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	23	theme	milk/	1225:1229	arg1	g/mL					1255:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL	1211:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis)	1211:1270	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	23	theme	milk/	1225:1229	arg1	agilis					1264:1269	L. agilis	1261:1269	L. agilis	1261:1269	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	24	theme	protectants	1116:1126	arg1	combination					1101:1111	The optimal combination	1089:1111	The optimal combination of protectants	1089:1126	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	24	theme	protectants	1116:1126	arg1	trehalose					1181:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose	1132:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius)	1132:1205	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	10	25	theme	extensive	1452:1460	arg1	protection					1462:1471	an extensive protection	1449:1471	an extensive protection	1449:1471	Our results indicate that the chosen protectants exerted an extensive protection on strains during the storage.					
31505895	7	26	theme	quadratic	913:921	arg1	model					934:938	A quadratic polynomial model	911:938	A quadratic polynomial model	911:938	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	3	27	theme	production	438:447	arg1	parameters					449:458	the production parameters	434:458	the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis)	434:535	In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis).					
31505895	8	28	theme	milk/	1146:1150	arg1	combination					1101:1111	The optimal combination	1089:1111	The optimal combination of protectants	1089:1126	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	28	theme	milk/	1146:1150	arg1	trehalose					1181:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose	1132:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius)	1132:1205	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	7	29	theme	skim	985:988	arg1	factors					976:982	three protective factors	959:982	three protective factors (skim milk, sucrose, and trehalose)	959:1018	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	7	29	theme	skim	985:988	arg1	milk					990:993	skim milk	985:993	skim milk	985:993	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	2	30	theme	production	327:336	arg1	optimization					303:314	technical optimization	293:314	technical optimization of biomass production	293:336	The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific.					
31505895	11	31	theme	room	1670:1673	arg1	temperature					1675:1685	room temperature	1670:1685	room temperature	1670:1685	Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.					
31505895	0	32	theme	Parameters	27:36	arg1	Optimization					0:11	Optimization	0:11	Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive.	0:90	Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive.					
31505895	8	33	theme	0.14g/mL	1132:1139	arg1	combination					1101:1111	The optimal combination	1089:1111	The optimal combination of protectants	1089:1126	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	33	theme	0.14g/mL	1132:1139	arg1	trehalose					1181:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose	1132:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius)	1132:1205	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	6	34	theme	surface	826:832	arg1	methodology					834:844	the response surface methodology	813:844	the response surface methodology	813:844	For the freeze-drying process, the response surface methodology was applied to optimize the composition of cryoprotective media.					
31505895	2	35	theme	biomass	319:325	arg1	production					327:336	biomass production	319:336	biomass production	319:336	The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific.					
31505895	2	36	from	viability	260:268	arg1	feed					273:276	feed	273:276	feed	273:276	The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific.					
31505895	1	37	theme	animal	95:100	arg1	nutrition					102:110	animal nutrition	95:110	animal nutrition	95:110	In animal nutrition, probiotics are considered as desirable alternatives to antibiotic growth promoters.					
31505895	4	38	theme	preferred	598:606	arg1	substrates					608:617	preferred substrates	598:617	preferred substrates that boosted biomass production in lab-scale fermentations	598:676	Carbohydrate utilization of both strains was determined and preferred substrates that boosted biomass production in lab-scale fermentations were selected.					
31505895	5	39	theme	easier	754:759	arg1	production					770:779	easier scale-up production	754:779	easier scale-up production	754:779	The strains showed good aerobic tolerance, which resulted in easier scale-up production.					
31505895	0	40	theme	Probiotic	42:50	arg1	Strains					66:72	Probiotic Lactobacillus Strains	42:72	Probiotic Lactobacillus Strains as Feed Additive	42:89	Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive.					
31505895	5	41	theme	scale-up	761:768	arg1	production					770:779	easier scale-up production	754:779	easier scale-up production	754:779	The strains showed good aerobic tolerance, which resulted in easier scale-up production.					
31505895	9	42	theme	in-feed	1304:1310	arg1	stabilities					1312:1322	the in-feed stabilities	1300:1322	the in-feed stabilities of the probiotic strains	1300:1347	Furthermore, the in-feed stabilities of the probiotic strains were evaluated under different conditions.					
31505895	3	43	theme	broiler-derived	468:482	arg1	lactobacilli					494:505	two broiler-derived probiotic lactobacilli	464:505	two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis)	464:535	In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis).					
31505895	8	44	theme	sucrose/	1241:1248	arg1	g/mL					1255:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL	1211:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis)	1211:1270	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	44	theme	sucrose/	1241:1248	arg1	agilis					1264:1269	L. agilis	1261:1269	L. agilis	1261:1269	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	45	theme	skim	1141:1144	arg1	combination					1101:1111	The optimal combination	1089:1111	The optimal combination of protectants	1089:1126	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	45	theme	skim	1141:1144	arg1	trehalose					1181:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose	1132:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius)	1132:1205	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	46	theme	g/mL	1236:1239	arg1	g/mL					1255:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL	1211:1258	0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis)	1211:1270	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	46	theme	g/mL	1236:1239	arg1	agilis					1264:1269	L. agilis	1261:1269	L. agilis	1261:1269	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	6	47	theme	response	817:824	arg1	methodology					834:844	the response surface methodology	813:844	the response surface methodology	813:844	For the freeze-drying process, the response surface methodology was applied to optimize the composition of cryoprotective media.					
31505895	3	48	theme	probiotic	484:492	arg1	lactobacilli					494:505	two broiler-derived probiotic lactobacilli	464:505	two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis)	464:535	In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis).					
31505895	8	49	theme	g/mL	1157:1160	arg1	combination					1101:1111	The optimal combination	1089:1111	The optimal combination of protectants	1089:1126	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	49	theme	g/mL	1157:1160	arg1	trehalose					1181:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose	1132:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius)	1132:1205	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	2	50	theme	storage	360:366	arg1	capacities					368:377	processing and storage capacities	345:377	processing and storage capacities	345:377	The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific.					
31505895	0	51	theme	Lactobacillus	52:64	arg1	Strains					66:72	Probiotic Lactobacillus Strains	42:72	Probiotic Lactobacillus Strains as Feed Additive	42:89	Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive.					
31505895	4	52	theme	strains	571:577	arg1	utilization					551:561	Carbohydrate utilization	538:561	Carbohydrate utilization of both strains	538:577	Carbohydrate utilization of both strains was determined and preferred substrates that boosted biomass production in lab-scale fermentations were selected.					
31505895	2	53	theme	probiotics	223:232	arg1	effects					212:218	The beneficial effects	197:218	The beneficial effects of probiotics	197:232	The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific.					
31505895	7	54	theme	optimal	1039:1045	arg1	conditions					1055:1064	the optimal working conditions	1035:1064	the optimal working conditions for maximum viability	1035:1086	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	3	55	theme	L.	526:527	arg1	agilis					529:534	L. agilis	526:534	L. agilis	526:534	In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis).					
31505895	11	56	theme	Lactobacillus	1588:1600	arg1	strains					1602:1608	both Lactobacillus strains	1583:1608	both Lactobacillus strains	1583:1608	Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.					
31505895	7	57	theme	maximum	1070:1076	arg1	viability					1078:1086	maximum viability	1070:1086	maximum viability	1070:1086	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	6	58	theme	media	904:908	arg1	composition					874:884	the composition	870:884	the composition of cryoprotective media	870:908	For the freeze-drying process, the response surface methodology was applied to optimize the composition of cryoprotective media.					
31505895	7	59	theme	working	1047:1053	arg1	conditions					1055:1064	the optimal working conditions	1035:1064	the optimal working conditions for maximum viability	1035:1086	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	2	60	theme	processing	345:354	arg1	capacities					368:377	processing and storage capacities	345:377	processing and storage capacities	345:377	The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific.					
31505895	11	61	theme	strains	1533:1539	arg1	storage					1518:1524	only storage	1513:1524	only storage of the strains at 4 °C	1513:1547	Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.					
31505895	8	62	theme	sucrose/	1162:1169	arg1	combination					1101:1111	The optimal combination	1089:1111	The optimal combination of protectants	1089:1126	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	62	theme	sucrose/	1162:1169	arg1	trehalose					1181:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose	1132:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius)	1132:1205	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	6	63	theme	cryoprotective	889:902	arg1	media					904:908	cryoprotective media	889:908	cryoprotective media	889:908	For the freeze-drying process, the response surface methodology was applied to optimize the composition of cryoprotective media.					
31505895	11	64	theme	protectant	1624:1633	arg1	matrix					1635:1640	the employed protectant matrix	1611:1640	the employed protectant matrix	1611:1640	Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.					
31505895	6	65	theme	freeze-drying	790:802	arg1	process					804:810	the freeze-drying process	786:810	the freeze-drying process	786:810	For the freeze-drying process, the response surface methodology was applied to optimize the composition of cryoprotective media.					
31505895	2	66	theme	beneficial	201:210	arg1	effects					212:218	The beneficial effects	197:218	The beneficial effects of probiotics	197:232	The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific.					
31505895	3	67	dep	lactobacilli	494:505	arg1	salivarius					511:520	L. salivarius	508:520	L. salivarius	508:520	In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis).					
31505895	3	67	dep	lactobacilli	494:505	arg1	agilis					529:534	L. agilis	526:534	L. agilis	526:534	In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis).					
31505895	1	68	theme	desirable	142:150	arg1	alternatives					152:163	desirable alternatives	142:163	desirable alternatives to antibiotic growth promoters	142:194	In animal nutrition, probiotics are considered as desirable alternatives to antibiotic growth promoters.					
31505895	1	68	theme	desirable	142:150	arg1	probiotics					113:122	probiotics	113:122	probiotics	113:122	In animal nutrition, probiotics are considered as desirable alternatives to antibiotic growth promoters.					
31505895	7	69	theme	protective	965:974	arg1	trehalose					1009:1017	trehalose	1009:1017	trehalose	1009:1017	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	7	69	theme	protective	965:974	arg1	sucrose					996:1002	sucrose	996:1002	sucrose	996:1002	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	7	69	theme	protective	965:974	arg1	factors					976:982	three protective factors	959:982	three protective factors (skim milk, sucrose, and trehalose)	959:1018	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	7	69	theme	protective	965:974	arg1	milk					990:993	skim milk	985:993	skim milk	985:993	A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability.					
31505895	11	70	theme	employed	1615:1622	arg1	matrix					1635:1640	the employed protectant matrix	1611:1640	the employed protectant matrix	1611:1640	Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.					
31505895	8	71	theme	g/mL	1176:1179	arg1	combination					1101:1111	The optimal combination	1089:1111	The optimal combination of protectants	1089:1126	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	8	71	theme	g/mL	1176:1179	arg1	trehalose					1181:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose	1132:1189	0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius)	1132:1205	The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively.					
31505895	0	72	theme	Production	16:25	arg1	Parameters					27:36	Production Parameters	16:36	Production Parameters	16:36	Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive.					
31505895	4	73	theme	lab-scale	654:662	arg1	fermentations					664:676	lab-scale fermentations	654:676	lab-scale fermentations	654:676	Carbohydrate utilization of both strains was determined and preferred substrates that boosted biomass production in lab-scale fermentations were selected.					
31604806	10	0	mod	modified	1584:1591	arg3	inulin					1596:1601	inulin	1596:1601	inulin	1596:1601	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	10	0	mod	modified	1584:1591	arg1	compositions					1472:1483	compositions	1472:1483	compositions	1472:1483	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	10	0	mod	modified	1584:1591	arg1	status					1461:1466	liver redox status	1449:1466	liver redox status	1449:1466	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	5	1	from	metabolism	796:805	arg1	mice					865:868	LDL receptor-deficient mice	842:868	LDL receptor-deficient mice	842:868	We studied the effects of inulin on lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice using lipidomics and transcriptomics.					
31604806	7	2	theme	plasma	1131:1136	arg1	ratio					1142:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	Compared with controls, inulin-fed mice displayed a decreased C16:0/C24:0 plasma Cer ratio and lower levels of circulating Cers associated with VLDL and LDL.					
31604806	4	3	theme	protective	703:712	arg1	processes					714:722	these protective processes	697:722	these protective processes	697:722	However, the mechanisms involved in these protective processes also are not well understood.					
31604806	2	4	theme	Cer	486:488	arg1	levels					490:495	circulating Cer levels	474:495	circulating Cer levels	474:495	Although the mechanisms involved are likely complex, these associations suggest that lowering circulating Cer levels could be protective against cardiovascular diseases.					
31604806	7	5	theme	Cer	1138:1140	arg1	ratio					1142:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	Compared with controls, inulin-fed mice displayed a decreased C16:0/C24:0 plasma Cer ratio and lower levels of circulating Cers associated with VLDL and LDL.					
31604806	7	6	theme	C16:0/C24:0	1119:1129	arg1	ratio					1142:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	Compared with controls, inulin-fed mice displayed a decreased C16:0/C24:0 plasma Cer ratio and lower levels of circulating Cers associated with VLDL and LDL.					
31604806	1	7	theme	disease	371:377	arg1	events					314:319	cardiac events	306:319	cardiac events	306:319	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	1	7	theme	disease	371:377	arg1	mortality					330:338	mortality	330:338	mortality	330:338	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	1	7	theme	disease	371:377	arg1	result					345:350	a result	343:350	a result of coronary artery disease	343:377	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	7	8	theme	decreased	1109:1117	arg1	ratio					1142:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	Compared with controls, inulin-fed mice displayed a decreased C16:0/C24:0 plasma Cer ratio and lower levels of circulating Cers associated with VLDL and LDL.					
31604806	6	9	theme	atherogenic	991:1001	arg1	diet					1003:1006	an atherogenic diet	988:1006	mice an atherogenic diet supplemented with inulin or cellulose (control)	983:1054	Plasma and tissues were collected at 10 days and/or 12 weeks after feeding mice an atherogenic diet supplemented with inulin or cellulose (control).					
31604806	2	10	theme	cardiovascular	525:538	arg1	diseases					540:547	cardiovascular diseases	525:547	cardiovascular diseases	525:547	Although the mechanisms involved are likely complex, these associations suggest that lowering circulating Cer levels could be protective against cardiovascular diseases.					
31604806	5	11	with	metabolism	796:805	arg1	model					814:818	a model	812:818	a model of atherosclerosis	812:837	We studied the effects of inulin on lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice using lipidomics and transcriptomics.					
31604806	7	12	theme	lower	1152:1156	arg1	levels					1158:1163	lower levels	1152:1163	lower levels of circulating Cers associated with VLDL and LDL	1152:1212	Compared with controls, inulin-fed mice displayed a decreased C16:0/C24:0 plasma Cer ratio and lower levels of circulating Cers associated with VLDL and LDL.					
31604806	3	13	theme	dietary	562:568	arg1	inulin					586:591	inulin	586:591	inulin	586:591	Conversely, dietary fibers, such as inulin, have been reported to promote cardiovascular and metabolic health.					
31604806	3	13	theme	dietary	562:568	arg1	fibers					570:575	dietary fibers	562:575	dietary fibers	562:575	Conversely, dietary fibers, such as inulin, have been reported to promote cardiovascular and metabolic health.					
31604806	10	14	theme	NSMase	1557:1562	arg1	activity					1564:1571	NSMase activity	1557:1571	NSMase activity	1557:1571	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	8	15	theme	inulin-fed	1341:1350	arg1	mice					1352:1355	inulin-fed mice	1341:1355	inulin-fed mice	1341:1355	Liver transcriptomic analysis revealed that Smpd3, a gene that encodes neutral SMase (NSMase), was downregulated by 2-fold in inulin-fed mice.					
31604806	10	16	theme	liver	1449:1453	arg1	status					1461:1466	liver redox status	1449:1466	liver redox status	1449:1466	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	1	17	theme	coronary	355:362	arg1	disease					371:377	coronary artery disease	355:377	coronary artery disease	355:377	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	5	18	theme	inulin	780:785	arg1	effects					769:775	the effects	765:775	the effects of inulin on lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice	765:868	We studied the effects of inulin on lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice using lipidomics and transcriptomics.					
31604806	7	19	theme	Cers	1180:1183	arg1	ratio					1142:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	a decreased C16:0/C24:0 plasma Cer ratio	1107:1146	Compared with controls, inulin-fed mice displayed a decreased C16:0/C24:0 plasma Cer ratio and lower levels of circulating Cers associated with VLDL and LDL.					
31604806	7	19	theme	Cers	1180:1183	arg1	levels					1158:1163	lower levels	1152:1163	lower levels of circulating Cers associated with VLDL and LDL	1152:1212	Compared with controls, inulin-fed mice displayed a decreased C16:0/C24:0 plasma Cer ratio and lower levels of circulating Cers associated with VLDL and LDL.					
31604806	1	20	theme	artery	364:369	arg1	disease					371:377	coronary artery disease	355:377	coronary artery disease	355:377	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	7	21	theme	inulin-fed	1081:1090	arg1	mice					1092:1095	inulin-fed mice	1081:1095	inulin-fed mice	1081:1095	Compared with controls, inulin-fed mice displayed a decreased C16:0/C24:0 plasma Cer ratio and lower levels of circulating Cers associated with VLDL and LDL.					
31604806	1	22	theme	increased	188:196	arg1	risk					198:201	increased risk	188:201	increased risk of cardiometabolic diseases	188:229	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	0	23	theme	Dietary	0:6	arg1	inulin					8:13	Dietary inulin	0:13	Dietary inulin	0:13	Dietary inulin decreases circulating ceramides by suppressing neutral sphingomyelinase expression and activity in mice.					
31604806	1	24	theme	metabolic	270:278	arg1	dysfunction					280:290	metabolic dysfunction	270:290	metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease	270:377	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	10	25	theme	major	1528:1532	arg1	factors					1534:1540	the major factors	1524:1540	the major factors that determine NSMase activity	1524:1571	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	10	25	theme	major	1528:1532	arg1	phosphatidylserine					1488:1505	phosphatidylserine	1488:1505	phosphatidylserine	1488:1505	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	0	26	theme	circulating	25:35	arg1	ceramides					37:45	circulating ceramides	25:45	circulating ceramides	25:45	Dietary inulin decreases circulating ceramides by suppressing neutral sphingomyelinase expression and activity in mice.					
31604806	10	27	theme	phosphatidylserine	1488:1505	arg1	compositions					1472:1483	compositions	1472:1483	compositions	1472:1483	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	10	27	theme	phosphatidylserine	1488:1505	arg1	status					1461:1466	liver redox status	1449:1466	liver redox status	1449:1466	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	5	28	theme	lipid	790:794	arg1	metabolism					796:805	lipid metabolism	790:805	lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice	790:868	We studied the effects of inulin on lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice using lipidomics and transcriptomics.					
31604806	5	29	theme	atherosclerosis	823:837	arg1	model					814:818	a model	812:818	a model of atherosclerosis	812:837	We studied the effects of inulin on lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice using lipidomics and transcriptomics.					
31604806	7	30	theme	circulating	1168:1178	arg1	Cers					1180:1183	circulating Cers	1168:1183	circulating Cers associated with VLDL and LDL	1168:1212	Compared with controls, inulin-fed mice displayed a decreased C16:0/C24:0 plasma Cer ratio and lower levels of circulating Cers associated with VLDL and LDL.					
31604806	9	31	theme	inulin-fed	1402:1411	arg1	mice					1413:1416	inulin-fed mice	1402:1416	inulin-fed mice	1402:1416	Hepatic NSMase activity was 3-fold lower in inulin-fed mice than in controls.					
31604806	0	32	from	activity	102:109	arg1	mice					114:117	mice	114:117	mice	114:117	Dietary inulin decreases circulating ceramides by suppressing neutral sphingomyelinase expression and activity in mice.					
31604806	1	33	theme	Elevated	120:127	arg1	levels					141:146	Elevated circulating levels	120:146	Elevated circulating levels of ceramides (Cers)	120:166	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	1	34	theme	cardiometabolic	206:220	arg1	diseases					222:229	cardiometabolic diseases	206:229	cardiometabolic diseases	206:229	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	2	35	theme	circulating	474:484	arg1	levels					490:495	circulating Cer levels	474:495	circulating Cer levels	474:495	Although the mechanisms involved are likely complex, these associations suggest that lowering circulating Cer levels could be protective against cardiovascular diseases.					
31604806	8	36	theme	neutral	1286:1292	arg1	gene					1268:1271	a gene	1266:1271	a gene that encodes neutral SMase (NSMase)	1266:1307	Liver transcriptomic analysis revealed that Smpd3, a gene that encodes neutral SMase (NSMase), was downregulated by 2-fold in inulin-fed mice.					
31604806	8	36	theme	neutral	1286:1292	arg1	NSMase					1301:1306	NSMase	1301:1306	NSMase	1301:1306	Liver transcriptomic analysis revealed that Smpd3, a gene that encodes neutral SMase (NSMase), was downregulated by 2-fold in inulin-fed mice.					
31604806	8	36	theme	neutral	1286:1292	arg1	SMase					1294:1298	neutral SMase	1286:1298	neutral SMase (NSMase)	1286:1307	Liver transcriptomic analysis revealed that Smpd3, a gene that encodes neutral SMase (NSMase), was downregulated by 2-fold in inulin-fed mice.					
31604806	5	37	theme	LDL	842:844	arg1	mice					865:868	LDL receptor-deficient mice	842:868	LDL receptor-deficient mice	842:868	We studied the effects of inulin on lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice using lipidomics and transcriptomics.					
31604806	1	38	theme	diseases	222:229	arg1	risk					198:201	increased risk	188:201	increased risk of cardiometabolic diseases	188:229	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	11	39	theme	plasma	1676:1681	arg1	levels					1687:1692	plasma Cer levels	1676:1692	plasma Cer levels	1676:1692	Taken together, these results showed that, in mice, inulin can decrease plasma Cer levels through reductions in NSMase expression and activity, suggesting a mechanism by which fiber could reduce cardiometabolic disease risk.					
31604806	1	40	theme	cardiac	306:312	arg1	events					314:319	cardiac events	306:319	cardiac events	306:319	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	1	40	theme	cardiac	306:312	arg1	mortality					330:338	mortality	330:338	mortality	330:338	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	1	40	theme	cardiac	306:312	arg1	result					345:350	a result	343:350	a result of coronary artery disease	343:377	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	1	41	theme	causative	252:260	arg1	role					262:265	a causative role	250:265	a causative role	250:265	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	0	42	from	expression	87:96	arg1	mice					114:117	mice	114:117	mice	114:117	Dietary inulin decreases circulating ceramides by suppressing neutral sphingomyelinase expression and activity in mice.					
31604806	5	43	theme	receptor-deficient	846:863	arg1	mice					865:868	LDL receptor-deficient mice	842:868	LDL receptor-deficient mice	842:868	We studied the effects of inulin on lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice using lipidomics and transcriptomics.					
31604806	0	44	theme	neutral	62:68	arg1	sphingomyelinase					70:85	neutral sphingomyelinase	62:85	neutral sphingomyelinase expression	62:96	Dietary inulin decreases circulating ceramides by suppressing neutral sphingomyelinase expression and activity in mice.					
31604806	11	45	theme	cardiometabolic	1799:1813	arg1	risk					1823:1826	cardiometabolic disease risk	1799:1826	cardiometabolic disease risk	1799:1826	Taken together, these results showed that, in mice, inulin can decrease plasma Cer levels through reductions in NSMase expression and activity, suggesting a mechanism by which fiber could reduce cardiometabolic disease risk.					
31604806	11	46	from	reductions	1702:1711	arg1	activity					1738:1745	activity	1738:1745	activity	1738:1745	Taken together, these results showed that, in mice, inulin can decrease plasma Cer levels through reductions in NSMase expression and activity, suggesting a mechanism by which fiber could reduce cardiometabolic disease risk.					
31604806	11	46	from	reductions	1702:1711	arg1	expression					1723:1732	NSMase expression	1716:1732	NSMase expression	1716:1732	Taken together, these results showed that, in mice, inulin can decrease plasma Cer levels through reductions in NSMase expression and activity, suggesting a mechanism by which fiber could reduce cardiometabolic disease risk.					
31604806	3	47	theme	metabolic	643:651	arg1	health					653:658	cardiovascular and metabolic health	624:658	cardiovascular and metabolic health	624:658	Conversely, dietary fibers, such as inulin, have been reported to promote cardiovascular and metabolic health.					
31604806	1	48	theme	circulating	129:139	arg1	levels					141:146	Elevated circulating levels	120:146	Elevated circulating levels of ceramides (Cers)	120:166	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	8	49	theme	transcriptomic	1221:1234	arg1	analysis					1236:1243	Liver transcriptomic analysis	1215:1243	Liver transcriptomic analysis	1215:1243	Liver transcriptomic analysis revealed that Smpd3, a gene that encodes neutral SMase (NSMase), was downregulated by 2-fold in inulin-fed mice.					
31604806	9	50	theme	Hepatic	1358:1364	arg1	activity					1373:1380	Hepatic NSMase activity	1358:1380	Hepatic NSMase activity	1358:1380	Hepatic NSMase activity was 3-fold lower in inulin-fed mice than in controls.					
31604806	10	51	theme	species	1515:1521	arg1	compositions					1472:1483	compositions	1472:1483	compositions	1472:1483	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	10	51	theme	species	1515:1521	arg1	status					1461:1466	liver redox status	1449:1466	liver redox status	1449:1466	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	5	52	from	effects	769:775	arg1	metabolism					796:805	lipid metabolism	790:805	lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice	790:868	We studied the effects of inulin on lipid metabolism with a model of atherosclerosis in LDL receptor-deficient mice using lipidomics and transcriptomics.					
31604806	10	53	theme	redox	1455:1459	arg1	status					1461:1466	liver redox status	1449:1466	liver redox status	1449:1466	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	8	54	theme	Liver	1215:1219	arg1	analysis					1236:1243	Liver transcriptomic analysis	1215:1243	Liver transcriptomic analysis	1215:1243	Liver transcriptomic analysis revealed that Smpd3, a gene that encodes neutral SMase (NSMase), was downregulated by 2-fold in inulin-fed mice.					
31604806	9	55	theme	NSMase	1366:1371	arg1	activity					1373:1380	Hepatic NSMase activity	1358:1380	Hepatic NSMase activity	1358:1380	Hepatic NSMase activity was 3-fold lower in inulin-fed mice than in controls.					
31604806	10	56	theme	FFA	1511:1513	arg1	species					1515:1521	FFA species	1511:1521	FFA species	1511:1521	Furthermore, liver redox status and compositions of phosphatidylserine and FFA species, the major factors that determine NSMase activity, were also modified by inulin.					
31604806	11	57	theme	disease	1815:1821	arg1	risk					1823:1826	cardiometabolic disease risk	1799:1826	cardiometabolic disease risk	1799:1826	Taken together, these results showed that, in mice, inulin can decrease plasma Cer levels through reductions in NSMase expression and activity, suggesting a mechanism by which fiber could reduce cardiometabolic disease risk.					
31604806	11	58	theme	NSMase	1716:1721	arg1	expression					1723:1732	NSMase expression	1716:1732	NSMase expression	1716:1732	Taken together, these results showed that, in mice, inulin can decrease plasma Cer levels through reductions in NSMase expression and activity, suggesting a mechanism by which fiber could reduce cardiometabolic disease risk.					
31604806	6	59	dep	mice	983:986	arg1	diet					1003:1006	an atherogenic diet	988:1006	mice an atherogenic diet supplemented with inulin or cellulose (control)	983:1054	Plasma and tissues were collected at 10 days and/or 12 weeks after feeding mice an atherogenic diet supplemented with inulin or cellulose (control).					
31604806	3	60	theme	cardiovascular	624:637	arg1	health					653:658	cardiovascular and metabolic health	624:658	cardiovascular and metabolic health	624:658	Conversely, dietary fibers, such as inulin, have been reported to promote cardiovascular and metabolic health.					
31604806	1	61	theme	ceramides	151:159	arg1	levels					141:146	Elevated circulating levels	120:146	Elevated circulating levels of ceramides (Cers)	120:166	Elevated circulating levels of ceramides (Cers) are associated with increased risk of cardiometabolic diseases, and Cers may play a causative role in metabolic dysfunction that precedes cardiac events, such as mortality as a result of coronary artery disease.					
31604806	11	62	theme	Cer	1683:1685	arg1	levels					1687:1692	plasma Cer levels	1676:1692	plasma Cer levels	1676:1692	Taken together, these results showed that, in mice, inulin can decrease plasma Cer levels through reductions in NSMase expression and activity, suggesting a mechanism by which fiber could reduce cardiometabolic disease risk.					
31604806	0	63	theme	sphingomyelinase	70:85	arg1	expression					87:96	neutral sphingomyelinase expression	62:96	neutral sphingomyelinase expression	62:96	Dietary inulin decreases circulating ceramides by suppressing neutral sphingomyelinase expression and activity in mice.					
30902314	3	0	theme	notable	721:727	arg1	potential					744:752	notable DNA protection potential	721:752	notable DNA protection potential	721:752	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	1	theme	DPPH	491:494	arg1	IC50 = 6.81 µg/mL					525:541	IC50 = 6.81 µg/mL	525:541	IC50 = 6.81 µg/mL	525:541	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	1	theme	DPPH	491:494	arg1	activity					515:522	strong in vitro DPPH radical scavenging activity	475:522	strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL)	475:542	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	1	2	theme	sodium	227:232	arg1	alginate					234:241	sodium alginate	227:241	sodium alginate coating on the bacterial and oxidative stability of cheese	227:300	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	1	3	theme	refrigerated	321:332	arg1	storage					334:340	refrigerated storage	321:340	refrigerated storage	321:340	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	6	4	from	%	1044:1044	arg1	enrichment					978:987	The enrichment	974:987	The enrichment	974:987	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	5	5	theme	mice	941:944	arg1	LD50 = 976.2 mg/kg					953:970	LD50 = 976.2 mg/kg	953:970	LD50 = 976.2 mg/kg	953:970	The acute toxicity of PSEO was determined using mice model (LD50 = 976.2 mg/kg).					
30902314	5	5	theme	mice	941:944	arg1	model					946:950	mice model	941:950	mice model (LD50 = 976.2 mg/kg)	941:971	The acute toxicity of PSEO was determined using mice model (LD50 = 976.2 mg/kg).					
30902314	3	6	theme	radical	496:502	arg1	IC50 = 6.81 µg/mL					525:541	IC50 = 6.81 µg/mL	525:541	IC50 = 6.81 µg/mL	525:541	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	6	theme	radical	496:502	arg1	activity					515:522	strong in vitro DPPH radical scavenging activity	475:522	strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL)	475:542	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	4	7	theme	antibacterial	791:803	arg1	activity					805:812	potent antibacterial activity	784:812	potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL)	784:890	Additionally, PSEO displayed potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL).					
30902314	3	8	theme	bleaching	556:564	arg1	IC50 = 206 µg/mL					578:593	IC50 = 206 µg/mL	578:593	IC50 = 206 µg/mL	578:593	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	8	theme	bleaching	556:564	arg1	inhibition					566:575	β-carotene bleaching inhibition	545:575	β-carotene bleaching inhibition (IC50 = 206 µg/mL)	545:594	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	6	9	with	enrichment	978:987	arg1	PSEO					1021:1024	PSEO	1021:1024	PSEO	1021:1024	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	4	10	theme	potent	784:789	arg1	activity					805:812	potent antibacterial activity	784:812	potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL)	784:890	Additionally, PSEO displayed potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL).					
30902314	3	11	dep	strong	475:480	arg1	in					482:483	in	482:483	in	482:483	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	1	12	from	effect	156:161	arg1	alginate					234:241	sodium alginate	227:241	sodium alginate coating on the bacterial and oxidative stability of cheese	227:300	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	3	13	dep	in	482:483	arg1	vitro					485:489	vitro	485:489	vitro	485:489	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	1	14	theme	oil	197:199	arg1	addition					208:215	Pimpinella saxifraga essential oil (PSEO) addition	166:215	Pimpinella saxifraga essential oil (PSEO) addition (1-3%)	166:222	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	1	14	theme	oil	197:199	arg1	%					221:221	1-3%	218:221	1-3%	218:221	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	1	15	theme	saxifraga	177:185	arg1	addition					208:215	Pimpinella saxifraga essential oil (PSEO) addition	166:215	Pimpinella saxifraga essential oil (PSEO) addition (1-3%)	166:222	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	1	15	theme	saxifraga	177:185	arg1	%					221:221	1-3%	218:221	1-3%	218:221	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	0	16	theme	cheese	111:116	arg1	preservation					118:129	cheese preservation	111:129	cheese preservation	111:129	Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive.					
30902314	3	17	theme	DNA	729:731	arg1	potential					744:752	notable DNA protection potential	721:752	notable DNA protection potential	721:752	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	1	18	theme	essential	187:195	arg1	addition					208:215	Pimpinella saxifraga essential oil (PSEO) addition	166:215	Pimpinella saxifraga essential oil (PSEO) addition (1-3%)	166:222	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	1	18	theme	essential	187:195	arg1	%					221:221	1-3%	218:221	1-3%	218:221	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	2	19	theme	main	439:442	arg1	p-anisaldehyde					415:428	p-anisaldehyde	415:428	p-anisaldehyde	415:428	The GC-HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components.					
30902314	2	19	theme	main	439:442	arg1	pseudoisoeugenol					394:409	pseudoisoeugenol	394:409	pseudoisoeugenol	394:409	The GC-HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components.					
30902314	2	19	theme	main	439:442	arg1	anethole					384:391	anethole	384:391	anethole	384:391	The GC-HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components.					
30902314	2	19	theme	main	439:442	arg1	components					444:453	the main components	435:453	the main components	435:453	The GC-HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components.					
30902314	3	20	theme	protection	733:742	arg1	potential					744:752	notable DNA protection potential	721:752	notable DNA protection potential	721:752	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	6	21	theme	unpleased	1189:1197	arg1	flavor					1199:1204	unpleased flavor	1189:1204	unpleased flavor for consumers	1189:1218	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	2	22	theme	PSEO	367:370	arg1	analysis					355:362	The GC-HRMS analysis	343:362	The GC-HRMS analysis of PSEO	343:370	The GC-HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components.					
30902314	6	23	theme	weight	1092:1097	arg1	loss					1099:1102	the weight loss	1088:1102	the weight loss	1088:1102	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	1	24	theme	bacterial	258:266	arg1	stability					282:290	the bacterial and oxidative stability	254:290	the bacterial and oxidative stability of cheese	254:300	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	3	25	theme	β-carotene	545:554	arg1	IC50 = 206 µg/mL					578:593	IC50 = 206 µg/mL	578:593	IC50 = 206 µg/mL	578:593	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	25	theme	β-carotene	545:554	arg1	inhibition					566:575	β-carotene bleaching inhibition	545:575	β-carotene bleaching inhibition (IC50 = 206 µg/mL)	545:594	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	1	26	theme	PSEO	202:205	arg1	addition					208:215	Pimpinella saxifraga essential oil (PSEO) addition	166:215	Pimpinella saxifraga essential oil (PSEO) addition (1-3%)	166:222	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	1	26	theme	PSEO	202:205	arg1	%					221:221	1-3%	218:221	1-3%	218:221	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	6	27	theme	coating	1008:1014	arg1	enrichment					978:987	The enrichment	974:987	The enrichment	974:987	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	3	28	theme	total	641:645	arg1	equivalent					705:714	213.96 ± 11.12 µmol/mL α-tocopherol equivalent	669:714	213.96 ± 11.12 µmol/mL α-tocopherol equivalent	669:714	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	28	theme	total	641:645	arg1	activity					659:666	total antioxidant activity	641:666	total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent)	641:715	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	1	29	theme	Pimpinella	166:175	arg1	addition					208:215	Pimpinella saxifraga essential oil (PSEO) addition	166:215	Pimpinella saxifraga essential oil (PSEO) addition (1-3%)	166:222	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	1	29	theme	Pimpinella	166:175	arg1	%					221:221	1-3%	218:221	1-3%	218:221	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	1	30	theme	oxidative	272:280	arg1	stability					282:290	the bacterial and oxidative stability	254:290	the bacterial and oxidative stability of cheese	254:300	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	3	31	theme	antioxidant	647:657	arg1	equivalent					705:714	213.96 ± 11.12 µmol/mL α-tocopherol equivalent	669:714	213.96 ± 11.12 µmol/mL α-tocopherol equivalent	669:714	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	31	theme	antioxidant	647:657	arg1	activity					659:666	total antioxidant activity	641:666	total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent)	641:715	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	6	32	theme	alginate	999:1006	arg1	coating					1008:1014	sodium alginate coating	992:1014	sodium alginate coating	992:1014	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	1	33	theme	addition	208:215	arg1	effect					156:161	The effect	152:161	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese	152:300	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	0	34	theme	antioxidant	31:41	arg1	activities					43:52	antioxidant activities	31:52	antioxidant activities	31:52	Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive.					
30902314	6	35	theme	sodium	992:997	arg1	coating					1008:1014	sodium alginate coating	992:1014	sodium alginate coating	992:1014	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	5	36	theme	PSEO	915:918	arg1	toxicity					903:910	The acute toxicity	893:910	The acute toxicity of PSEO	893:918	The acute toxicity of PSEO was determined using mice model (LD50 = 976.2 mg/kg).					
30902314	3	37	theme	ferric	597:602	arg1	power					613:617	ferric reducing power	597:617	ferric reducing power (EC50 = 35.20 µg/mL)	597:638	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	37	theme	ferric	597:602	arg1	EC50 = 35.20 µg/mL					620:637	EC50 = 35.20 µg/mL	620:637	EC50 = 35.20 µg/mL	620:637	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	5	38	theme	acute	897:901	arg1	toxicity					903:910	The acute toxicity	893:910	The acute toxicity of PSEO	893:918	The acute toxicity of PSEO was determined using mice model (LD50 = 976.2 mg/kg).					
30902314	0	39	theme	Pimpinella	57:66	arg1	antibacterial					13:25	antibacterial	13:25	antibacterial	13:25	Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive.					
30902314	0	39	theme	Pimpinella	57:66	arg1	activities					43:52	antioxidant activities	31:52	antioxidant activities	31:52	Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive.					
30902314	0	39	theme	Pimpinella	57:66	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive.					
30902314	1	40	theme	cheese	295:300	arg1	stability					282:290	the bacterial and oxidative stability	254:290	the bacterial and oxidative stability of cheese	254:300	The effect of Pimpinella saxifraga essential oil (PSEO) addition (1-3%) in sodium alginate coating on the bacterial and oxidative stability of cheese was studied during refrigerated storage.					
30902314	3	41	theme	213.96 ± 11.12 µmol/mL	669:690	arg1	equivalent					705:714	213.96 ± 11.12 µmol/mL α-tocopherol equivalent	669:714	213.96 ± 11.12 µmol/mL α-tocopherol equivalent	669:714	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	41	theme	213.96 ± 11.12 µmol/mL	669:690	arg1	activity					659:666	total antioxidant activity	641:666	total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent)	641:715	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	6	42	theme	oxidative	1147:1155	arg1	stability					1171:1179	oxidative and bacterial stability	1147:1179	oxidative and bacterial stability	1147:1179	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	3	43	theme	α-tocopherol	692:703	arg1	equivalent					705:714	213.96 ± 11.12 µmol/mL α-tocopherol equivalent	669:714	213.96 ± 11.12 µmol/mL α-tocopherol equivalent	669:714	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	43	theme	α-tocopherol	692:703	arg1	activity					659:666	total antioxidant activity	641:666	total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent)	641:715	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	4	44	theme	Gram-negative	844:856	arg1	bacteria					858:865	3 Gram-negative bacteria	842:865	3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL)	842:890	Additionally, PSEO displayed potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL).					
30902314	4	44	theme	Gram-negative	844:856	arg1	MICs = 0.78-3.12 mg/mL					868:889	MICs = 0.78-3.12 mg/mL	868:889	MICs = 0.78-3.12 mg/mL	868:889	Additionally, PSEO displayed potent antibacterial activity against 3 Gram-positive and 3 Gram-negative bacteria (MICs = 0.78-3.12 mg/mL).					
30902314	6	45	theme	cheese	1056:1061	arg1	preservation					1063:1074	cheese preservation	1056:1074	cheese preservation	1056:1074	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	3	46	theme	strong	475:480	arg1	IC50 = 6.81 µg/mL					525:541	IC50 = 6.81 µg/mL	525:541	IC50 = 6.81 µg/mL	525:541	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	46	theme	strong	475:480	arg1	activity					515:522	strong in vitro DPPH radical scavenging activity	475:522	strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL)	475:542	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	0	47	theme	essential	78:86	arg1	oil					88:90	essential oil	78:90	essential oil	78:90	Composition, antibacterial and antioxidant activities of Pimpinella saxifraga essential oil and application to cheese preservation as coating additive.					
30902314	3	48	theme	reducing	604:611	arg1	power					613:617	ferric reducing power	597:617	ferric reducing power (EC50 = 35.20 µg/mL)	597:638	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	48	theme	reducing	604:611	arg1	EC50 = 35.20 µg/mL					620:637	EC50 = 35.20 µg/mL	620:637	EC50 = 35.20 µg/mL	620:637	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	49	theme	scavenging	504:513	arg1	IC50 = 6.81 µg/mL					525:541	IC50 = 6.81 µg/mL	525:541	IC50 = 6.81 µg/mL	525:541	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	3	49	theme	scavenging	504:513	arg1	activity					515:522	strong in vitro DPPH radical scavenging activity	475:522	strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL)	475:542	The PSEO exhibited strong in vitro DPPH radical scavenging activity (IC50 = 6.81 µg/mL), β-carotene bleaching inhibition (IC50 = 206 µg/mL), ferric reducing power (EC50 = 35.20 µg/mL), total antioxidant activity (213.96 ± 11.12 µmol/mL α-tocopherol equivalent) and notable DNA protection potential.					
30902314	6	50	theme	bacterial	1161:1169	arg1	stability					1171:1179	oxidative and bacterial stability	1147:1179	oxidative and bacterial stability	1147:1179	The enrichment of sodium alginate coating with PSEO, particularly at 3%, improved cheese preservation by reducing the weight loss, preserving the pH and color and enhancing oxidative and bacterial stability without unpleased flavor for consumers.					
30902314	2	51	theme	GC-HRMS	347:353	arg1	analysis					355:362	The GC-HRMS analysis	343:362	The GC-HRMS analysis of PSEO	343:370	The GC-HRMS analysis of PSEO showed that anethole, pseudoisoeugenol and p-anisaldehyde were the main components.					
29892745	8	0	from	effects	1221:1227	arg1	pancreas					1254:1261	the pancreas	1250:1261	the pancreas	1250:1261	The protective effects of bifidobacteria on the pancreas were strongly correlated with those on blood glucose.					
29892745	7	1	theme	B.	1134:1135	arg1	strain					1106:1111	only one strain	1097:1111	only one strain of B. longum and the B. adolescentis	1097:1148	However, only one strain of B. longum and the B. adolescentis displayed significant regulation of blood lipid levels.					
29892745	10	2	theme	metabolic	1555:1563	arg1	syndrome					1565:1572	metabolic syndrome	1555:1572	metabolic syndrome	1555:1572	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	2	3	theme	syndrome	374:381	arg1	amelioration					348:359	amelioration	348:359	amelioration of metabolic syndrome	348:381	Intervention with probiotics is a novel strategy for amelioration of metabolic syndrome, which is believed to regulate the gut microbiota composition to some extent.					
29892745	4	4	theme	bifidobacteria	619:632	arg1	strains					608:614	Seven strains	602:614	Seven strains of bifidobacteria from four species	602:650	Seven strains of bifidobacteria from four species were individually administered to rats fed a HFHS diet for 12 weeks.					
29892745	4	5	from	species	644:650	arg1	strains					608:614	Seven strains	602:614	Seven strains of bifidobacteria from four species	602:650	Seven strains of bifidobacteria from four species were individually administered to rats fed a HFHS diet for 12 weeks.					
29892745	3	6	theme	diet-induced	533:544	arg1	disorders					556:564	diet-induced metabolic disorders	533:564	diet-induced metabolic disorders	533:564	We investigated the relationship amongst bifidobacteria treatment, HFHS diet-induced metabolic disorders and the gut microbiota composition.					
29892745	5	7	from	effects	767:773	arg1	recovery					782:789	the recovery	778:789	the recovery of metabolic disorders and gut microbiota dysbiosis	778:841	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	2	8	theme	metabolic	364:372	arg1	syndrome					374:381	metabolic syndrome	364:381	metabolic syndrome	364:381	Intervention with probiotics is a novel strategy for amelioration of metabolic syndrome, which is believed to regulate the gut microbiota composition to some extent.					
29892745	9	9	with	relationship	1414:1425	arg1	symptoms					1432:1439	symptoms	1432:1439	symptoms of metabolic disorders	1432:1462	Furthermore, the influence of bifidobacteria on gut microbiota dysbiosis also showed a potential relationship with symptoms of metabolic disorders.					
29892745	3	10	theme	gut	574:576	arg1	composition					589:599	the gut microbiota composition	570:599	the gut microbiota composition	570:599	We investigated the relationship amongst bifidobacteria treatment, HFHS diet-induced metabolic disorders and the gut microbiota composition.					
29892745	1	11	theme	related	266:272	arg1	disorders					284:292	related metabolic disorders	266:292	related metabolic disorders	266:292	Host gut microbiota dysbiosis occurs for multiple reasons and is often accompanied by chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders.					
29892745	10	12	theme	tissue	1625:1630	arg1	disorders					1614:1622	glucose and lipid metabolism disorders	1585:1622	glucose and lipid metabolism disorders	1585:1622	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	10	12	theme	tissue	1625:1630	arg1	damage					1632:1637	tissue damage	1625:1637	tissue damage	1625:1637	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	3	13	theme	microbiota	578:587	arg1	composition					589:599	the gut microbiota composition	570:599	the gut microbiota composition	570:599	We investigated the relationship amongst bifidobacteria treatment, HFHS diet-induced metabolic disorders and the gut microbiota composition.					
29892745	1	14	theme	metabolic	274:282	arg1	disorders					284:292	related metabolic disorders	266:292	related metabolic disorders	266:292	Host gut microbiota dysbiosis occurs for multiple reasons and is often accompanied by chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders.					
29892745	11	15	used	used	1737:1740	arg2	strain					1674:1679	This strain	1669:1679	This strain	1669:1679	This strain, coupled with other prebiotics and probiotics, could be used as a potential treatment approach for metabolic syndrome induced by a HFHS diet.					
29892745	11	15	used	used	1737:1740	arg2	approach					1767:1774	a potential treatment approach	1745:1774	a potential treatment approach for metabolic syndrome induced by a HFHS diet	1745:1820	This strain, coupled with other prebiotics and probiotics, could be used as a potential treatment approach for metabolic syndrome induced by a HFHS diet.					
29892745	7	16	theme	significant	1160:1170	arg1	regulation					1172:1181	significant regulation	1160:1181	significant regulation of blood lipid levels	1160:1203	However, only one strain of B. longum and the B. adolescentis displayed significant regulation of blood lipid levels.					
29892745	9	17	theme	bifidobacteria	1347:1360	arg1	influence					1334:1342	the influence	1330:1342	the influence of bifidobacteria on gut microbiota dysbiosis	1330:1388	Furthermore, the influence of bifidobacteria on gut microbiota dysbiosis also showed a potential relationship with symptoms of metabolic disorders.					
29892745	5	18	theme	microbiota	822:831	arg1	dysbiosis					833:841	gut microbiota dysbiosis	818:841	gut microbiota dysbiosis	818:841	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	10	19	theme	outstanding	1522:1532	arg1	ability					1534:1540	an outstanding ability	1519:1540	an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis	1519:1666	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	4	20	theme	HFHS	697:700	arg1	diet					702:705	a HFHS diet	695:705	a HFHS diet for 12 weeks	695:718	Seven strains of bifidobacteria from four species were individually administered to rats fed a HFHS diet for 12 weeks.					
29892745	7	21	theme	levels	1198:1203	arg1	regulation					1172:1181	significant regulation	1160:1181	significant regulation of blood lipid levels	1160:1203	However, only one strain of B. longum and the B. adolescentis displayed significant regulation of blood lipid levels.					
29892745	5	22	theme	gut	818:820	arg1	dysbiosis					833:841	gut microbiota dysbiosis	818:841	gut microbiota dysbiosis	818:841	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	10	23	theme	B.	1489:1490	arg1	Z25					1505:1507	B. adolescentis Z25	1489:1507	B. adolescentis Z25	1489:1507	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	10	24	theme	metabolism	1603:1612	arg1	dysbiosis					1658:1666	gut microbiota dysbiosis	1643:1666	gut microbiota dysbiosis	1643:1666	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	10	24	theme	metabolism	1603:1612	arg1	disorders					1614:1622	glucose and lipid metabolism disorders	1585:1622	glucose and lipid metabolism disorders	1585:1622	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	10	24	theme	metabolism	1603:1612	arg1	damage					1632:1637	tissue damage	1625:1637	tissue damage	1625:1637	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	2	25	theme	novel	329:333	arg1	Intervention					295:306	Intervention	295:306	Intervention with probiotics	295:322	Intervention with probiotics is a novel strategy for amelioration of metabolic syndrome, which is believed to regulate the gut microbiota composition to some extent.					
29892745	2	25	theme	novel	329:333	arg1	strategy					335:342	a novel strategy	327:342	a novel strategy	327:342	Intervention with probiotics is a novel strategy for amelioration of metabolic syndrome, which is believed to regulate the gut microbiota composition to some extent.					
29892745	10	26	theme	gut	1643:1645	arg1	dysbiosis					1658:1666	gut microbiota dysbiosis	1643:1666	gut microbiota dysbiosis	1643:1666	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	10	26	theme	gut	1643:1645	arg1	disorders					1614:1622	glucose and lipid metabolism disorders	1585:1622	glucose and lipid metabolism disorders	1585:1622	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	10	27	theme	microbiota	1647:1656	arg1	dysbiosis					1658:1666	gut microbiota dysbiosis	1643:1666	gut microbiota dysbiosis	1643:1666	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	10	27	theme	microbiota	1647:1656	arg1	disorders					1614:1622	glucose and lipid metabolism disorders	1585:1622	glucose and lipid metabolism disorders	1585:1622	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	9	28	theme	gut	1365:1367	arg1	dysbiosis					1380:1388	gut microbiota dysbiosis	1365:1388	gut microbiota dysbiosis	1365:1388	Furthermore, the influence of bifidobacteria on gut microbiota dysbiosis also showed a potential relationship with symptoms of metabolic disorders.					
29892745	6	29	theme	breve	1033:1037	arg1	strains					1019:1025	two strains	1015:1025	two strains of B. breve	1015:1037	Bifidobacterium longum, B. adolescentis and B. bifidum seemed to affect the blood glucose balance, whilst two strains of B. breve showed extremely different effects in this area.					
29892745	1	30	theme	chronic	194:200	arg1	inflammation					202:213	chronic inflammation	194:213	chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders	194:292	Host gut microbiota dysbiosis occurs for multiple reasons and is often accompanied by chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders.					
29892745	7	31	theme	blood	1186:1190	arg1	levels					1198:1203	blood lipid levels	1186:1203	blood lipid levels	1186:1203	However, only one strain of B. longum and the B. adolescentis displayed significant regulation of blood lipid levels.					
29892745	9	32	theme	microbiota	1369:1378	arg1	dysbiosis					1380:1388	gut microbiota dysbiosis	1365:1388	gut microbiota dysbiosis	1365:1388	Furthermore, the influence of bifidobacteria on gut microbiota dysbiosis also showed a potential relationship with symptoms of metabolic disorders.					
29892745	11	33	theme	HFHS	1812:1815	arg1	diet					1817:1820	a HFHS diet	1810:1820	a HFHS diet	1810:1820	This strain, coupled with other prebiotics and probiotics, could be used as a potential treatment approach for metabolic syndrome induced by a HFHS diet.					
29892745	0	34	dep	inter-	70:75	arg1	differences					95:105	differences	95:105	differences	95:105	Bifidobacteria attenuate the development of metabolic disorders, with inter- and intra-species differences.					
29892745	7	35	theme	lipid	1192:1196	arg1	levels					1198:1203	blood lipid levels	1186:1203	blood lipid levels	1186:1203	However, only one strain of B. longum and the B. adolescentis displayed significant regulation of blood lipid levels.					
29892745	1	36	theme	gut	113:115	arg1	dysbiosis					128:136	Host gut microbiota dysbiosis	108:136	Host gut microbiota dysbiosis	108:136	Host gut microbiota dysbiosis occurs for multiple reasons and is often accompanied by chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders.					
29892745	2	37	theme	microbiota	422:431	arg1	composition					433:443	the gut microbiota composition	414:443	the gut microbiota composition	414:443	Intervention with probiotics is a novel strategy for amelioration of metabolic syndrome, which is believed to regulate the gut microbiota composition to some extent.					
29892745	0	38	theme	metabolic	44:52	arg1	disorders					54:62	metabolic disorders	44:62	metabolic disorders	44:62	Bifidobacteria attenuate the development of metabolic disorders, with inter- and intra-species differences.					
29892745	5	39	theme	various	759:765	arg1	effects					767:773	various effects	759:773	various effects on the recovery of metabolic disorders and gut microbiota dysbiosis	759:841	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	2	40	theme	gut	418:420	arg1	composition					433:443	the gut microbiota composition	414:443	the gut microbiota composition	414:443	Intervention with probiotics is a novel strategy for amelioration of metabolic syndrome, which is believed to regulate the gut microbiota composition to some extent.					
29892745	5	41	theme	dysbiosis	833:841	arg1	recovery					782:789	the recovery	778:789	the recovery of metabolic disorders and gut microbiota dysbiosis	778:841	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	6	42	theme	glucose	991:997	arg1	balance					999:1005	the blood glucose balance	981:1005	the blood glucose balance	981:1005	Bifidobacterium longum, B. adolescentis and B. bifidum seemed to affect the blood glucose balance, whilst two strains of B. breve showed extremely different effects in this area.					
29892745	6	43	theme	B.	1030:1031	arg1	breve					1033:1037	B. breve	1030:1037	B. breve	1030:1037	Bifidobacterium longum, B. adolescentis and B. bifidum seemed to affect the blood glucose balance, whilst two strains of B. breve showed extremely different effects in this area.					
29892745	7	44	dep	B.	1134:1135	arg1	adolescentis					1137:1148	adolescentis	1137:1148	adolescentis	1137:1148	However, only one strain of B. longum and the B. adolescentis displayed significant regulation of blood lipid levels.					
29892745	9	45	theme	disorders	1454:1462	arg1	symptoms					1432:1439	symptoms	1432:1439	symptoms of metabolic disorders	1432:1462	Furthermore, the influence of bifidobacteria on gut microbiota dysbiosis also showed a potential relationship with symptoms of metabolic disorders.					
29892745	8	46	theme	blood	1302:1306	arg1	glucose					1308:1314	blood glucose	1302:1314	blood glucose	1302:1314	The protective effects of bifidobacteria on the pancreas were strongly correlated with those on blood glucose.					
29892745	10	47	dep	B.	1489:1490	arg1	adolescentis					1492:1503	adolescentis	1492:1503	adolescentis	1492:1503	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	6	48	theme	blood	985:989	arg1	balance					999:1005	the blood glucose balance	981:1005	the blood glucose balance	981:1005	Bifidobacterium longum, B. adolescentis and B. bifidum seemed to affect the blood glucose balance, whilst two strains of B. breve showed extremely different effects in this area.					
29892745	8	49	theme	bifidobacteria	1232:1245	arg1	effects					1221:1227	The protective effects	1206:1227	The protective effects of bifidobacteria on the pancreas	1206:1261	The protective effects of bifidobacteria on the pancreas were strongly correlated with those on blood glucose.					
29892745	10	50	theme	lipid	1597:1601	arg1	metabolism					1603:1612	lipid metabolism	1597:1612	lipid metabolism	1597:1612	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	11	51	theme	metabolic	1780:1788	arg1	syndrome					1790:1797	metabolic syndrome	1780:1797	metabolic syndrome induced by a HFHS diet	1780:1820	This strain, coupled with other prebiotics and probiotics, could be used as a potential treatment approach for metabolic syndrome induced by a HFHS diet.					
29892745	1	52	theme	microbiota	117:126	arg1	dysbiosis					128:136	Host gut microbiota dysbiosis	108:136	Host gut microbiota dysbiosis	108:136	Host gut microbiota dysbiosis occurs for multiple reasons and is often accompanied by chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders.					
29892745	0	53	theme	disorders	54:62	arg1	development					29:39	the development	25:39	the development of metabolic disorders	25:62	Bifidobacteria attenuate the development of metabolic disorders, with inter- and intra-species differences.					
29892745	9	54	theme	potential	1404:1412	arg1	relationship					1414:1425	a potential relationship	1402:1425	a potential relationship with symptoms of metabolic disorders	1402:1462	Furthermore, the influence of bifidobacteria on gut microbiota dysbiosis also showed a potential relationship with symptoms of metabolic disorders.					
29892745	7	55	theme	longum	1119:1124	arg1	strain					1106:1111	only one strain	1097:1111	only one strain of B. longum and the B. adolescentis	1097:1148	However, only one strain of B. longum and the B. adolescentis displayed significant regulation of blood lipid levels.					
29892745	5	56	theme	Various	721:727	arg1	strains					744:750	Various bifidobacteria strains	721:750	Various bifidobacteria strains	721:750	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	1	57	theme	multiple	149:156	arg1	reasons					158:164	multiple reasons	149:164	multiple reasons	149:164	Host gut microbiota dysbiosis occurs for multiple reasons and is often accompanied by chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders.					
29892745	9	58	from	influence	1334:1342	arg1	dysbiosis					1380:1388	gut microbiota dysbiosis	1365:1388	gut microbiota dysbiosis	1365:1388	Furthermore, the influence of bifidobacteria on gut microbiota dysbiosis also showed a potential relationship with symptoms of metabolic disorders.					
29892745	1	59	theme	Host	108:111	arg1	dysbiosis					128:136	Host gut microbiota dysbiosis	108:136	Host gut microbiota dysbiosis	108:136	Host gut microbiota dysbiosis occurs for multiple reasons and is often accompanied by chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders.					
29892745	1	60	theme	high-fat-high-sucrose	228:248	arg1	HFHS					251:254	HFHS	251:254	HFHS	251:254	Host gut microbiota dysbiosis occurs for multiple reasons and is often accompanied by chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders.					
29892745	1	60	theme	high-fat-high-sucrose	228:248	arg1	diet					257:260	a high-fat-high-sucrose (HFHS) diet	226:260	a high-fat-high-sucrose (HFHS) diet	226:260	Host gut microbiota dysbiosis occurs for multiple reasons and is often accompanied by chronic inflammation induced by a high-fat-high-sucrose (HFHS) diet and related metabolic disorders.					
29892745	5	61	theme	bifidobacteria	729:742	arg1	strains					744:750	Various bifidobacteria strains	721:750	Various bifidobacteria strains	721:750	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	11	62	theme	potential	1747:1755	arg1	strain					1674:1679	This strain	1669:1679	This strain	1669:1679	This strain, coupled with other prebiotics and probiotics, could be used as a potential treatment approach for metabolic syndrome induced by a HFHS diet.					
29892745	11	62	theme	potential	1747:1755	arg1	approach					1767:1774	a potential treatment approach	1745:1774	a potential treatment approach for metabolic syndrome induced by a HFHS diet	1745:1820	This strain, coupled with other prebiotics and probiotics, could be used as a potential treatment approach for metabolic syndrome induced by a HFHS diet.					
29892745	3	63	theme	bifidobacteria	502:515	arg1	treatment					517:525	bifidobacteria treatment	502:525	bifidobacteria treatment	502:525	We investigated the relationship amongst bifidobacteria treatment, HFHS diet-induced metabolic disorders and the gut microbiota composition.					
29892745	6	64	theme	different	1056:1064	arg1	effects					1066:1072	extremely different effects	1046:1072	extremely different effects	1046:1072	Bifidobacterium longum, B. adolescentis and B. bifidum seemed to affect the blood glucose balance, whilst two strains of B. breve showed extremely different effects in this area.					
29892745	5	65	theme	metabolic	794:802	arg1	disorders					804:812	metabolic disorders	794:812	metabolic disorders	794:812	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	11	66	theme	treatment	1757:1765	arg1	strain					1674:1679	This strain	1669:1679	This strain	1669:1679	This strain, coupled with other prebiotics and probiotics, could be used as a potential treatment approach for metabolic syndrome induced by a HFHS diet.					
29892745	11	66	theme	treatment	1757:1765	arg1	approach					1767:1774	a potential treatment approach	1745:1774	a potential treatment approach for metabolic syndrome induced by a HFHS diet	1745:1820	This strain, coupled with other prebiotics and probiotics, could be used as a potential treatment approach for metabolic syndrome induced by a HFHS diet.					
29892745	3	67	theme	metabolic	546:554	arg1	disorders					556:564	diet-induced metabolic disorders	533:564	diet-induced metabolic disorders	533:564	We investigated the relationship amongst bifidobacteria treatment, HFHS diet-induced metabolic disorders and the gut microbiota composition.					
29892745	10	68	theme	glucose	1585:1591	arg1	dysbiosis					1658:1666	gut microbiota dysbiosis	1643:1666	gut microbiota dysbiosis	1643:1666	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	10	68	theme	glucose	1585:1591	arg1	disorders					1614:1622	glucose and lipid metabolism disorders	1585:1622	glucose and lipid metabolism disorders	1585:1622	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	10	68	theme	glucose	1585:1591	arg1	damage					1632:1637	tissue damage	1625:1637	tissue damage	1625:1637	Of these seven strains, B. adolescentis Z25 displayed an outstanding ability to alleviate metabolic syndrome, including glucose and lipid metabolism disorders, tissue damage and gut microbiota dysbiosis.					
29892745	5	69	theme	disorders	804:812	arg1	recovery					782:789	the recovery	778:789	the recovery of metabolic disorders and gut microbiota dysbiosis	778:841	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	11	70	theme	other	1695:1699	arg1	prebiotics					1701:1710	other prebiotics	1695:1710	other prebiotics	1695:1710	This strain, coupled with other prebiotics and probiotics, could be used as a potential treatment approach for metabolic syndrome induced by a HFHS diet.					
29892745	5	71	dep	inter-	875:880	arg1	specific					899:906	specific	899:906	specific	899:906	Various bifidobacteria strains showed various effects on the recovery of metabolic disorders and gut microbiota dysbiosis, and these effects seemed to be inter- or intra-species specific.					
29892745	8	72	theme	protective	1210:1219	arg1	effects					1221:1227	The protective effects	1206:1227	The protective effects of bifidobacteria on the pancreas	1206:1261	The protective effects of bifidobacteria on the pancreas were strongly correlated with those on blood glucose.					
29892745	9	73	theme	metabolic	1444:1452	arg1	disorders					1454:1462	metabolic disorders	1444:1462	metabolic disorders	1444:1462	Furthermore, the influence of bifidobacteria on gut microbiota dysbiosis also showed a potential relationship with symptoms of metabolic disorders.					
29892745	2	74	with	Intervention	295:306	arg1	probiotics					313:322	probiotics	313:322	probiotics	313:322	Intervention with probiotics is a novel strategy for amelioration of metabolic syndrome, which is believed to regulate the gut microbiota composition to some extent.					
30984946	8	0	theme	adhesion	1684:1691	arg1	assay					1693:1697	the bacterial adhesion assay	1670:1697	the bacterial adhesion assay	1670:1697	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	6	1	theme	synthesised	1228:1238	arg1	heteroclusters					1240:1253	The synthesised heteroclusters	1224:1253	The synthesised heteroclusters	1224:1253	The synthesised heteroclusters were tested in standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces.					
30984946	1	2	theme	Multivalent	113:123	arg1	interactions					146:157	Multivalent carbohydrate-protein interactions	113:157	Multivalent carbohydrate-protein interactions	113:157	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	1	2	theme	Multivalent	113:123	arg1	events					167:172	key events	163:172	key events in cell recognition processes	163:202	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	3	3	theme	ligand	768:773	arg1	orientation					775:785	relative ligand orientation	759:785	relative ligand orientation	759:785	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	6	4	theme	ConA	1356:1359	arg1	binding					1361:1367	ConA binding	1356:1367	ConA binding	1356:1367	The synthesised heteroclusters were tested in standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces.					
30984946	4	5	theme	distinct	887:894	arg1	ligands					909:915	two distinct carbohydrate ligands	883:915	two distinct carbohydrate ligands	883:915	Two enantiomeric scaffolds, derived from d- and l-serine, respectively, were glycosylated with two distinct carbohydrate ligands to obtain a library of pseudoenantiomeric glycoclusters.					
30984946	7	6	theme	bacterial	1546:1554	arg1	adhesion					1556:1563	FimH-mediated bacterial adhesion	1532:1563	FimH-mediated bacterial adhesion	1532:1563	A striking difference was observed between the potencies of the two pseudoenantiomeric glucose-containing glycoclusters as inhibitors of FimH-mediated bacterial adhesion.					
30984946	11	7	with	agreement	2027:2035	arg1	data					2055:2058	experimental data	2042:2058	experimental data obtained in the bacterial adhesion assays	2042:2100	Binding energies for the FimH carbohydrate recognition domain were calculated for both diastereomers and are in agreement with experimental data obtained in the bacterial adhesion assays.					
30984946	6	8	theme	FimH-mediated	1319:1331	arg1	adhesion					1343:1350	FimH-mediated bacterial adhesion	1319:1350	FimH-mediated bacterial adhesion	1319:1350	The synthesised heteroclusters were tested in standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces.					
30984946	2	9	from	level	539:543	arg1	understanding					486:498	a conclusive understanding	473:498	a conclusive understanding of glycan recognition at the molecular level	473:543	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	2	9	from	level	539:543	arg1	recognition					510:520	glycan recognition	503:520	glycan recognition at the molecular level	503:543	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	6	10	theme	standard	1270:1277	arg1	assays					1298:1303	standard binding-inhibition assays	1270:1303	standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces	1270:1392	The synthesised heteroclusters were tested in standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces.					
30984946	9	11	with	assays	1717:1722	arg1	ConA					1729:1732	ConA	1729:1732	ConA	1729:1732	In contrast, the assays with ConA showed no significant variation for all tested cluster pairs.					
30984946	5	12	theme	carbohydrate	1102:1113	arg1	portion					1115:1121	the second carbohydrate portion	1091:1121	the second carbohydrate portion	1091:1121	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	7	13	theme	glucose-containing	1482:1499	arg1	glycoclusters					1501:1513	the two pseudoenantiomeric glucose-containing glycoclusters	1455:1513	the two pseudoenantiomeric glucose-containing glycoclusters	1455:1513	A striking difference was observed between the potencies of the two pseudoenantiomeric glucose-containing glycoclusters as inhibitors of FimH-mediated bacterial adhesion.					
30984946	4	14	theme	pseudoenantiomeric	940:957	arg1	glycoclusters					959:971	pseudoenantiomeric glycoclusters	940:971	pseudoenantiomeric glycoclusters	940:971	Two enantiomeric scaffolds, derived from d- and l-serine, respectively, were glycosylated with two distinct carbohydrate ligands to obtain a library of pseudoenantiomeric glycoclusters.					
30984946	8	15	theme	diastereomeric	1580:1593	arg1	pairs					1608:1612	the other diastereomeric glycocluster pairs	1570:1612	the other diastereomeric glycocluster pairs	1570:1612	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	6	16	theme	binding-inhibition	1279:1296	arg1	assays					1298:1303	standard binding-inhibition assays	1270:1303	standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces	1270:1392	The synthesised heteroclusters were tested in standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces.					
30984946	5	17	contain	have	983:986	arg2	ligand					1036:1041	a specific ligand	1025:1041	a specific ligand for lectins FimH and ConA	1025:1067	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	5	17	contain	have	983:986	arg2	residue					1004:1010	an α-d-mannosyl residue	988:1010	an α-d-mannosyl residue in common	988:1020	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	5	17	contain	have	983:986	arg1	They					974:977	They	974:977	They	974:977	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	3	18	theme	carbohydrate-lectin	583:601	arg1	studies					615:621	carbohydrate-lectin recognition studies	583:621	carbohydrate-lectin recognition studies	583:621	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	3	19	theme	first	640:644	arg1	glycoclusters					676:688	the first heterobivalent diastereomeric glycoclusters	636:688	the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation	636:785	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	1	20	theme	cell	177:180	arg1	processes					194:202	cell recognition processes	177:202	cell recognition processes	177:202	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	8	21	theme	inhibitory	1622:1631	arg1	differences					1641:1651	smaller inhibitory potency differences	1614:1651	smaller inhibitory potency differences	1614:1651	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	6	22	gly	mannosylated	1372:1383	arg1	surfaces					1385:1392	mannosylated surfaces	1372:1392	mannosylated surfaces	1372:1392	The synthesised heteroclusters were tested in standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces.					
30984946	2	23	theme	recognition	510:520	arg1	understanding					486:498	a conclusive understanding	473:498	a conclusive understanding of glycan recognition at the molecular level	473:543	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	11	24	theme	carbohydrate	1945:1956	arg1	domain					1970:1975	the FimH carbohydrate recognition domain	1936:1975	the FimH carbohydrate recognition domain	1936:1975	Binding energies for the FimH carbohydrate recognition domain were calculated for both diastereomers and are in agreement with experimental data obtained in the bacterial adhesion assays.					
30984946	2	25	theme	multivalent	416:426	arg1	structures					428:437	multivalent structures	416:437	multivalent structures	416:437	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	8	26	located	detected	1658:1665	arg2	differences					1641:1651	smaller inhibitory potency differences	1614:1651	smaller inhibitory potency differences	1614:1651	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	8	26	located	detected	1658:1665	arg1	assay					1693:1697	the bacterial adhesion assay	1670:1697	the bacterial adhesion assay	1670:1697	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	2	27	from	understanding	486:498	arg1	level					539:543	the molecular level	525:543	the molecular level	525:543	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	11	28	theme	bacterial	2076:2084	arg1	assays					2095:2100	the bacterial adhesion assays	2072:2100	the bacterial adhesion assays	2072:2100	Binding energies for the FimH carbohydrate recognition domain were calculated for both diastereomers and are in agreement with experimental data obtained in the bacterial adhesion assays.					
30984946	0	29	theme	Pseudoenantiomeric	0:17	arg1	testing					48:54	testing	48:54	testing	48:54	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	0	29	theme	Pseudoenantiomeric	0:17	arg1	synthesis					34:42	synthesis	34:42	synthesis	34:42	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	0	29	theme	Pseudoenantiomeric	0:17	arg1	glycoclusters					19:31	Pseudoenantiomeric glycoclusters	0:31	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.	0:111	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	1	30	theme	carbohydrate-protein	125:144	arg1	interactions					146:157	Multivalent carbohydrate-protein interactions	113:157	Multivalent carbohydrate-protein interactions	113:157	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	1	30	theme	carbohydrate-protein	125:144	arg1	events					167:172	key events	163:172	key events in cell recognition processes	163:202	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	10	31	theme	diastereomeric	1826:1839	arg1	pair					1870:1873	the diastereomeric glucose-mannose glycocluster pair	1822:1873	the diastereomeric glucose-mannose glycocluster pair	1822:1873	The results obtained with the diastereomeric glucose-mannose glycocluster pair were rationalised by molecular docking.					
30984946	7	32	theme	striking	1397:1404	arg1	difference					1406:1415	A striking difference	1395:1415	A striking difference	1395:1415	A striking difference was observed between the potencies of the two pseudoenantiomeric glucose-containing glycoclusters as inhibitors of FimH-mediated bacterial adhesion.					
30984946	5	33	theme	β-d-glucosaminyl	1176:1191	arg1	residue					1193:1199	a β-d-glucosaminyl residue	1174:1199	a β-d-glucosaminyl residue as unspecific ligands	1174:1221	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	2	34	theme	conclusive	475:484	arg1	understanding					486:498	a conclusive understanding	473:498	a conclusive understanding of glycan recognition at the molecular level	473:543	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	3	35	theme	diastereomeric	661:674	arg1	glycoclusters					676:688	the first heterobivalent diastereomeric glycoclusters	636:688	the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation	636:785	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	2	36	theme	polyvalent	358:367	arg1	scaffold					369:376	a polyvalent scaffold	356:376	a polyvalent scaffold	356:376	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	5	37	from	residue	1004:1010	arg1	common					1015:1020	common	1015:1020	common	1015:1020	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	9	38	theme	cluster	1781:1787	arg1	pairs					1789:1793	all tested cluster pairs	1770:1793	all tested cluster pairs	1770:1793	In contrast, the assays with ConA showed no significant variation for all tested cluster pairs.					
30984946	3	39	theme	heteromultivalency	736:753	arg1	influence					718:726	the influence	714:726	the influence of both heteromultivalency and relative ligand orientation	714:785	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	6	40	theme	mannosylated	1372:1383	arg1	surfaces					1385:1392	mannosylated surfaces	1372:1392	mannosylated surfaces	1372:1392	The synthesised heteroclusters were tested in standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces.					
30984946	3	41	theme	orientation	775:785	arg1	influence					718:726	the influence	714:726	the influence of both heteromultivalency and relative ligand orientation	714:785	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	7	42	theme	adhesion	1556:1563	arg1	inhibitors					1518:1527	inhibitors	1518:1527	inhibitors of FimH-mediated bacterial adhesion	1518:1563	A striking difference was observed between the potencies of the two pseudoenantiomeric glucose-containing glycoclusters as inhibitors of FimH-mediated bacterial adhesion.					
30984946	3	43	theme	relative	759:766	arg1	orientation					775:785	relative ligand orientation	759:785	relative ligand orientation	759:785	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	6	44	theme	bacterial	1333:1341	arg1	adhesion					1343:1350	FimH-mediated bacterial adhesion	1319:1350	FimH-mediated bacterial adhesion	1319:1350	The synthesised heteroclusters were tested in standard binding-inhibition assays investigating FimH-mediated bacterial adhesion and ConA binding to mannosylated surfaces.					
30984946	3	45	theme	new	569:571	arg1	aspect					573:578	a new aspect	567:578	a new aspect to carbohydrate-lectin recognition studies	567:621	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	2	46	attach	attached	344:351	arg1	scaffold					369:376	a polyvalent scaffold	356:376	a polyvalent scaffold	356:376	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	2	46	attach	attached	344:351	arg2	ligand					337:342	the ligand	333:342	the ligand attached to a polyvalent scaffold	333:376	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	4	47	theme	carbohydrate	896:907	arg1	ligands					909:915	two distinct carbohydrate ligands	883:915	two distinct carbohydrate ligands	883:915	Two enantiomeric scaffolds, derived from d- and l-serine, respectively, were glycosylated with two distinct carbohydrate ligands to obtain a library of pseudoenantiomeric glycoclusters.					
30984946	7	48	theme	FimH-mediated	1532:1544	arg1	adhesion					1556:1563	FimH-mediated bacterial adhesion	1532:1563	FimH-mediated bacterial adhesion	1532:1563	A striking difference was observed between the potencies of the two pseudoenantiomeric glucose-containing glycoclusters as inhibitors of FimH-mediated bacterial adhesion.					
30984946	4	49	attach	derived	816:822	arg2	scaffolds					805:813	Two enantiomeric scaffolds	788:813	Two enantiomeric scaffolds	788:813	Two enantiomeric scaffolds, derived from d- and l-serine, respectively, were glycosylated with two distinct carbohydrate ligands to obtain a library of pseudoenantiomeric glycoclusters.					
30984946	4	49	attach	derived	816:822	arg1	l-serine					836:843	l-serine	836:843	l-serine	836:843	Two enantiomeric scaffolds, derived from d- and l-serine, respectively, were glycosylated with two distinct carbohydrate ligands to obtain a library of pseudoenantiomeric glycoclusters.					
30984946	4	49	attach	derived	816:822	arg1	d-					829:830	d-	829:830	d-	829:830	Two enantiomeric scaffolds, derived from d- and l-serine, respectively, were glycosylated with two distinct carbohydrate ligands to obtain a library of pseudoenantiomeric glycoclusters.					
30984946	2	50	theme	ligand	337:342	arg1	multiplicity					317:328	the multiplicity	313:328	the multiplicity of the ligand attached to a polyvalent scaffold	313:376	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	8	51	theme	other	1574:1578	arg1	pairs					1608:1612	the other diastereomeric glycocluster pairs	1570:1612	the other diastereomeric glycocluster pairs	1570:1612	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	3	52	theme	recognition	603:613	arg1	studies					615:621	carbohydrate-lectin recognition studies	583:621	carbohydrate-lectin recognition studies	583:621	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	1	53	theme	synthetic	250:258	arg1	glycomimetics					260:272	synthetic glycomimetics	250:272	synthetic glycomimetics	250:272	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	8	54	theme	glycocluster	1595:1606	arg1	pairs					1608:1612	the other diastereomeric glycocluster pairs	1570:1612	the other diastereomeric glycocluster pairs	1570:1612	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	7	55	theme	glycoclusters	1501:1513	arg1	potencies					1442:1450	the potencies	1438:1450	the potencies of the two pseudoenantiomeric glucose-containing glycoclusters as inhibitors of FimH-mediated bacterial adhesion	1438:1563	A striking difference was observed between the potencies of the two pseudoenantiomeric glucose-containing glycoclusters as inhibitors of FimH-mediated bacterial adhesion.					
30984946	5	56	dep	lectins	1047:1053	arg1	FimH					1055:1058	FimH	1055:1058	FimH	1055:1058	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	5	56	dep	lectins	1047:1053	arg1	lectins					1047:1053	lectins FimH and ConA	1047:1067	lectins FimH and ConA	1047:1067	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	5	56	dep	lectins	1047:1053	arg1	ConA					1064:1067	ConA	1064:1067	ConA	1064:1067	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	1	57	theme	key	163:165	arg1	interactions					146:157	Multivalent carbohydrate-protein interactions	113:157	Multivalent carbohydrate-protein interactions	113:157	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	1	57	theme	key	163:165	arg1	events					167:172	key events	163:172	key events in cell recognition processes	163:202	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	8	58	theme	smaller	1614:1620	arg1	differences					1641:1651	smaller inhibitory potency differences	1614:1651	smaller inhibitory potency differences	1614:1651	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	7	59	theme	pseudoenantiomeric	1463:1480	arg1	glycoclusters					1501:1513	the two pseudoenantiomeric glucose-containing glycoclusters	1455:1513	the two pseudoenantiomeric glucose-containing glycoclusters	1455:1513	A striking difference was observed between the potencies of the two pseudoenantiomeric glucose-containing glycoclusters as inhibitors of FimH-mediated bacterial adhesion.					
30984946	4	60	theme	glycoclusters	959:971	arg1	library					929:935	a library	927:935	a library of pseudoenantiomeric glycoclusters	927:971	Two enantiomeric scaffolds, derived from d- and l-serine, respectively, were glycosylated with two distinct carbohydrate ligands to obtain a library of pseudoenantiomeric glycoclusters.					
30984946	8	61	theme	potency	1633:1639	arg1	differences					1641:1651	smaller inhibitory potency differences	1614:1651	smaller inhibitory potency differences	1614:1651	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	10	62	theme	glycocluster	1857:1868	arg1	pair					1870:1873	the diastereomeric glucose-mannose glycocluster pair	1822:1873	the diastereomeric glucose-mannose glycocluster pair	1822:1873	The results obtained with the diastereomeric glucose-mannose glycocluster pair were rationalised by molecular docking.					
30984946	0	63	theme	carbohydrate-protein	78:97	arg1	interactions					99:110	carbohydrate-protein interactions	78:110	carbohydrate-protein interactions	78:110	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	5	64	theme	second	1095:1100	arg1	portion					1115:1121	the second carbohydrate portion	1091:1121	the second carbohydrate portion	1091:1121	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	1	65	theme	recognition	182:192	arg1	processes					194:202	cell recognition processes	177:202	cell recognition processes	177:202	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	0	66	from	synthesis	34:42	arg1	interactions					99:110	carbohydrate-protein interactions	78:110	carbohydrate-protein interactions	78:110	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	11	67	theme	Binding	1915:1921	arg1	energies					1923:1930	Binding energies	1915:1930	Binding energies for the FimH carbohydrate recognition domain	1915:1975	Binding energies for the FimH carbohydrate recognition domain were calculated for both diastereomers and are in agreement with experimental data obtained in the bacterial adhesion assays.					
30984946	2	68	theme	glycan	503:508	arg1	recognition					510:520	glycan recognition	503:520	glycan recognition at the molecular level	503:543	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	2	69	dep	multiplicity	317:328	arg1	i.e.					308:311	i.e.	308:311	i.e.	308:311	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	11	70	theme	recognition	1958:1968	arg1	domain					1970:1975	the FimH carbohydrate recognition domain	1936:1975	the FimH carbohydrate recognition domain	1936:1975	Binding energies for the FimH carbohydrate recognition domain were calculated for both diastereomers and are in agreement with experimental data obtained in the bacterial adhesion assays.					
30984946	8	71	theme	bacterial	1674:1682	arg1	assay					1693:1697	the bacterial adhesion assay	1670:1697	the bacterial adhesion assay	1670:1697	For the other diastereomeric glycocluster pairs smaller inhibitory potency differences were detected in the bacterial adhesion assay.					
30984946	11	72	theme	FimH	1940:1943	arg1	domain					1970:1975	the FimH carbohydrate recognition domain	1936:1975	the FimH carbohydrate recognition domain	1936:1975	Binding energies for the FimH carbohydrate recognition domain were calculated for both diastereomers and are in agreement with experimental data obtained in the bacterial adhesion assays.					
30984946	5	73	theme	specific	1027:1034	arg1	residue					1004:1010	an α-d-mannosyl residue	988:1010	an α-d-mannosyl residue in common	988:1020	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	5	73	theme	specific	1027:1034	arg1	ligand					1036:1041	a specific ligand	1025:1041	a specific ligand for lectins FimH and ConA	1025:1067	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	2	74	theme	molecular	529:537	arg1	level					539:543	the molecular level	525:543	the molecular level	525:543	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	0	75	from	testing	48:54	arg1	interactions					99:110	carbohydrate-protein interactions	78:110	carbohydrate-protein interactions	78:110	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	5	76	theme	α-d-mannosyl	991:1002	arg1	residue					1004:1010	an α-d-mannosyl residue	988:1010	an α-d-mannosyl residue in common	988:1020	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	5	76	theme	α-d-mannosyl	991:1002	arg1	ligand					1036:1041	a specific ligand	1025:1041	a specific ligand for lectins FimH and ConA	1025:1067	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	11	77	theme	adhesion	2086:2093	arg1	assays					2095:2100	the bacterial adhesion assays	2072:2100	the bacterial adhesion assays	2072:2100	Binding energies for the FimH carbohydrate recognition domain were calculated for both diastereomers and are in agreement with experimental data obtained in the bacterial adhesion assays.					
30984946	4	78	gly	glycosylated	865:876	arg1	scaffolds					805:813	Two enantiomeric scaffolds	788:813	Two enantiomeric scaffolds	788:813	Two enantiomeric scaffolds, derived from d- and l-serine, respectively, were glycosylated with two distinct carbohydrate ligands to obtain a library of pseudoenantiomeric glycoclusters.					
30984946	0	79	dep	glycoclusters	19:31	arg1	testing					48:54	testing	48:54	testing	48:54	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	0	79	dep	glycoclusters	19:31	arg1	synthesis					34:42	synthesis	34:42	synthesis	34:42	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	0	79	dep	glycoclusters	19:31	arg1	glycoclusters					19:31	Pseudoenantiomeric glycoclusters	0:31	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.	0:111	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	1	80	from	events	167:172	arg1	processes					194:202	cell recognition processes	177:202	cell recognition processes	177:202	Multivalent carbohydrate-protein interactions are key events in cell recognition processes and have been extensively studied by means of synthetic glycomimetics.					
30984946	4	81	theme	enantiomeric	792:803	arg1	scaffolds					805:813	Two enantiomeric scaffolds	788:813	Two enantiomeric scaffolds	788:813	Two enantiomeric scaffolds, derived from d- and l-serine, respectively, were glycosylated with two distinct carbohydrate ligands to obtain a library of pseudoenantiomeric glycoclusters.					
30984946	3	82	theme	heterobivalent	646:659	arg1	glycoclusters					676:688	the first heterobivalent diastereomeric glycoclusters	636:688	the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation	636:785	In this work, we add a new aspect to carbohydrate-lectin recognition studies by designing the first heterobivalent diastereomeric glycoclusters in order to investigate the influence of both heteromultivalency and relative ligand orientation.					
30984946	2	83	theme	structures	428:437	arg1	design					406:411	the design	402:411	the design of multivalent structures	402:437	To date, frequently the valency, i.e. the multiplicity of the ligand attached to a polyvalent scaffold, has been considered in the design of multivalent structures but these studies have not led to a conclusive understanding of glycan recognition at the molecular level.					
30984946	0	84	theme	heterobivalency	59:73	arg1	testing					48:54	testing	48:54	testing	48:54	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	0	84	theme	heterobivalency	59:73	arg1	synthesis					34:42	synthesis	34:42	synthesis	34:42	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	0	84	theme	heterobivalency	59:73	arg1	glycoclusters					19:31	Pseudoenantiomeric glycoclusters	0:31	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.	0:111	Pseudoenantiomeric glycoclusters: synthesis and testing of heterobivalency in carbohydrate-protein interactions.					
30984946	9	85	theme	significant	1744:1754	arg1	variation					1756:1764	no significant variation	1741:1764	no significant variation for all tested cluster pairs	1741:1793	In contrast, the assays with ConA showed no significant variation for all tested cluster pairs.					
30984946	11	86	theme	experimental	2042:2053	arg1	data					2055:2058	experimental data	2042:2058	experimental data obtained in the bacterial adhesion assays	2042:2100	Binding energies for the FimH carbohydrate recognition domain were calculated for both diastereomers and are in agreement with experimental data obtained in the bacterial adhesion assays.					
30984946	5	87	theme	unspecific	1204:1213	arg1	ligands					1215:1221	unspecific ligands	1204:1221	unspecific ligands	1204:1221	They all have an α-d-mannosyl residue in common as a specific ligand for lectins FimH and ConA, while they differ in the second carbohydrate portion, consisting of a β-d-glucosyl, a β-d-galactosyl or a β-d-glucosaminyl residue as unspecific ligands.					
30984946	10	88	theme	glucose-mannose	1841:1855	arg1	pair					1870:1873	the diastereomeric glucose-mannose glycocluster pair	1822:1873	the diastereomeric glucose-mannose glycocluster pair	1822:1873	The results obtained with the diastereomeric glucose-mannose glycocluster pair were rationalised by molecular docking.					
30984946	9	89	theme	tested	1774:1779	arg1	pairs					1789:1793	all tested cluster pairs	1770:1793	all tested cluster pairs	1770:1793	In contrast, the assays with ConA showed no significant variation for all tested cluster pairs.					
30984946	10	90	theme	molecular	1896:1904	arg1	docking					1906:1912	molecular docking	1896:1912	molecular docking	1896:1912	The results obtained with the diastereomeric glucose-mannose glycocluster pair were rationalised by molecular docking.					
31176120	0	0	theme	topical	93:99	arg1	hydrogel					101:108	a topical hydrogel	91:108	a topical hydrogel	91:108	DoE-based development, physicochemical characterization, and pharmacological evaluation of a topical hydrogel containing betamethasone dipropionate microemulsion.					
31176120	4	1	theme	BMD	949:951	arg1	flux					941:944	flux	941:944	flux	941:944	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	4	1	theme	BMD	949:951	arg1	deposition					926:935	skin deposition	921:935	skin deposition	921:935	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	7	2	from	flux	1584:1587	arg1	order					1613:1617	the following order	1599:1617	the following order	1599:1617	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	5	3	dep	properties	1092:1101	arg1	flux					1104:1107	flux	1104:1107	flux	1104:1107	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	3	dep	properties	1092:1101	arg1	deposition					1118:1127	skin deposition	1113:1127	skin deposition	1113:1127	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	3	dep	properties	1092:1101	arg1	properties					1092:1101	four properties	1087:1101	four properties: flux and skin deposition of BMD	1087:1134	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	6	4	theme	solid	1430:1434	arg1	SLN-hydrogel					1457:1468	SLN-hydrogel	1457:1468	SLN-hydrogel	1457:1468	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	6	4	theme	solid	1430:1434	arg1	nanoparticles					1442:1454	the hydrogel-containing solid lipid nanoparticles	1406:1454	the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel)	1406:1469	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	5	5	theme	BMD	1132:1134	arg1	hydration					1137:1145	hydration	1137:1145	hydration of stratum corneum	1137:1164	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	5	theme	BMD	1132:1134	arg1	flux					1104:1107	flux	1104:1107	flux	1104:1107	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	5	theme	BMD	1132:1134	arg1	deposition					1118:1127	skin deposition	1113:1127	skin deposition	1113:1127	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	5	theme	BMD	1132:1134	arg1	properties					1092:1101	four properties	1087:1101	four properties: flux and skin deposition of BMD	1087:1134	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	5	theme	BMD	1132:1134	arg1	properties					1183:1192	rheological properties	1171:1192	rheological properties of hydrogel-containing microemulsion (ME-hydrogel)	1171:1243	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	6	6	theme	carboxymethyl	1321:1333	arg1	cellulose					1335:1343	carboxymethyl cellulose	1321:1343	carboxymethyl cellulose as the hydrogel-forming agent	1321:1373	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	3	7	theme	microemulsion	713:725	arg1	size					735:738	microemulsion droplet size	713:738	microemulsion droplet size	713:738	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	7	theme	microemulsion	713:725	arg1	variables					668:676	three dependent variables	652:676	three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size)	652:739	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	6	8	theme	optimal	1296:1302	arg1	ME-hydrogel					1304:1314	optimal ME-hydrogel	1296:1314	optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent	1296:1373	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	2	9	theme	cellulose	395:403	arg1	derivatives					405:415	cellulose derivatives	395:415	cellulose derivatives	395:415	Second, a hydrogel-containing microemulsion was developed using cellulose derivatives, and its anti-inflammatory and skin irritation effects were evaluated.					
31176120	6	10	theme	hydrogel-containing	1410:1428	arg1	SLN-hydrogel					1457:1468	SLN-hydrogel	1457:1468	SLN-hydrogel	1457:1468	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	6	10	theme	hydrogel-containing	1410:1428	arg1	nanoparticles					1442:1454	the hydrogel-containing solid lipid nanoparticles	1406:1454	the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel)	1406:1469	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	7	11	theme	edema	1538:1542	arg1	inhibition					1544:1553	edema inhibition	1538:1553	edema inhibition	1538:1553	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	3	12	theme	droplet	727:733	arg1	size					735:738	microemulsion droplet size	713:738	microemulsion droplet size	713:738	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	12	theme	droplet	727:733	arg1	variables					668:676	three dependent variables	652:676	three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size)	652:739	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	13	theme	surfactant	617:626	arg1	ratio					608:612	ratio	608:612	ratio of surfactant to cosolvent	608:639	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	13	theme	surfactant	617:626	arg1	variables					582:590	two independent variables	566:590	two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS)	566:647	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	13	theme	surfactant	617:626	arg1	acid					599:602	oleic acid	593:602	oleic acid	593:602	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	0	14	theme	hydrogel	101:108	arg1	evaluation					77:86	pharmacological evaluation	61:86	pharmacological evaluation	61:86	DoE-based development, physicochemical characterization, and pharmacological evaluation of a topical hydrogel containing betamethasone dipropionate microemulsion.					
31176120	0	14	theme	hydrogel	101:108	arg1	development					10:20	DoE-based development	0:20	DoE-based development	0:20	DoE-based development, physicochemical characterization, and pharmacological evaluation of a topical hydrogel containing betamethasone dipropionate microemulsion.					
31176120	0	14	theme	hydrogel	101:108	arg1	characterization					39:54	physicochemical characterization	23:54	physicochemical characterization	23:54	DoE-based development, physicochemical characterization, and pharmacological evaluation of a topical hydrogel containing betamethasone dipropionate microemulsion.					
31176120	4	15	from	level	790:794	arg1	acid					776:779	oleic acid	770:779	oleic acid at a low level (coded with -1) and S/CoS at a high level	770:836	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	4	16	theme	low	786:788	arg1	level					790:794	a low level	784:794	a low level (coded with -1)	784:810	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	4	17	from	terms	912:916	arg1	it					883:884	it	883:884	it	883:884	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	4	17	from	terms	912:916	arg1	effective					899:907	effective	899:907	effective	899:907	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	0	18	theme	dipropionate	135:146	arg1	microemulsion					148:160	betamethasone dipropionate microemulsion	121:160	betamethasone dipropionate microemulsion	121:160	DoE-based development, physicochemical characterization, and pharmacological evaluation of a topical hydrogel containing betamethasone dipropionate microemulsion.					
31176120	3	19	theme	oleic	593:597	arg1	variables					582:590	two independent variables	566:590	two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS)	566:647	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	19	theme	oleic	593:597	arg1	acid					599:602	oleic acid	593:602	oleic acid	593:602	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	20	theme	central	502:508	arg1	design					520:525	Face-centered central composite design	488:525	Face-centered central composite design	488:525	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	0	21	theme	betamethasone	121:133	arg1	microemulsion					148:160	betamethasone dipropionate microemulsion	121:160	betamethasone dipropionate microemulsion	121:160	DoE-based development, physicochemical characterization, and pharmacological evaluation of a topical hydrogel containing betamethasone dipropionate microemulsion.					
31176120	5	22	theme	corneum	1158:1164	arg1	hydration					1137:1145	hydration	1137:1145	hydration of stratum corneum	1137:1164	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	22	theme	corneum	1158:1164	arg1	flux					1104:1107	flux	1104:1107	flux	1104:1107	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	22	theme	corneum	1158:1164	arg1	deposition					1118:1127	skin deposition	1113:1127	skin deposition	1113:1127	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	22	theme	corneum	1158:1164	arg1	properties					1092:1101	four properties	1087:1101	four properties: flux and skin deposition of BMD	1087:1134	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	22	theme	corneum	1158:1164	arg1	properties					1183:1192	rheological properties	1171:1192	rheological properties of hydrogel-containing microemulsion (ME-hydrogel)	1171:1243	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	23	theme	stratum	1150:1156	arg1	corneum					1158:1164	stratum corneum	1150:1164	stratum corneum	1150:1164	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	1	24	theme	primary	203:209	arg1	goals					211:215	two primary goals	199:215	two primary goals	199:215	This study was performed to achieve two primary goals: First, a microemulsion containing betamethasone dipropionate was optimized using the quality by design approach.					
31176120	5	25	theme	HPMC	987:990	arg1	derivatives					974:984	Different cellulose derivatives	954:984	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC)	954:1015	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	25	theme	HPMC	987:990	arg1	E6					992:993	HPMC E6	987:993	HPMC E6	987:993	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	4	26	from	level	832:836	arg1	acid					776:779	oleic acid	770:779	oleic acid at a low level (coded with -1) and S/CoS at a high level	770:836	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	2	27	theme	hydrogel-containing	341:359	arg1	microemulsion					361:373	a hydrogel-containing microemulsion	339:373	a hydrogel-containing microemulsion	339:373	Second, a hydrogel-containing microemulsion was developed using cellulose derivatives, and its anti-inflammatory and skin irritation effects were evaluated.					
31176120	7	28	theme	>	1648:1648	arg1	NLC-hydrogel					1650:1661	ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel	1620:1661	ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%)	1620:1677	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	7	28	theme	>	1648:1648	arg1	%					1676:1676	35.93 ± 7.22%	1664:1676	35.93 ± 7.22%	1664:1676	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	2	29	theme	anti-inflammatory	426:442	arg1	effects					464:470	its anti-inflammatory and skin irritation effects	422:470	its anti-inflammatory and skin irritation effects	422:470	Second, a hydrogel-containing microemulsion was developed using cellulose derivatives, and its anti-inflammatory and skin irritation effects were evaluated.					
31176120	0	30	theme	DoE-based	0:8	arg1	development					10:20	DoE-based development	0:20	DoE-based development	0:20	DoE-based development, physicochemical characterization, and pharmacological evaluation of a topical hydrogel containing betamethasone dipropionate microemulsion.					
31176120	3	31	theme	BMD	704:706	arg1	size					735:738	microemulsion droplet size	713:738	microemulsion droplet size	713:738	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	31	theme	BMD	704:706	arg1	flux					696:699	flux	696:699	flux of BMD	696:706	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	31	theme	BMD	704:706	arg1	variables					668:676	three dependent variables	652:676	three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size)	652:739	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	31	theme	BMD	704:706	arg1	deposition					684:693	skin deposition	679:693	skin deposition	679:693	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	32	from	impacts	555:561	arg1	size					735:738	microemulsion droplet size	713:738	microemulsion droplet size	713:738	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	32	from	impacts	555:561	arg1	flux					696:699	flux	696:699	flux of BMD	696:706	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	32	from	impacts	555:561	arg1	variables					668:676	three dependent variables	652:676	three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size)	652:739	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	32	from	impacts	555:561	arg1	deposition					684:693	skin deposition	679:693	skin deposition	679:693	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	4	33	theme	oleic	770:774	arg1	acid					776:779	oleic acid	770:779	oleic acid at a low level (coded with -1) and S/CoS at a high level	770:836	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	3	34	theme	Face-centered	488:500	arg1	design					520:525	Face-centered central composite design	488:525	Face-centered central composite design	488:525	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	0	35	theme	physicochemical	23:37	arg1	characterization					39:54	physicochemical characterization	23:54	physicochemical characterization	23:54	DoE-based development, physicochemical characterization, and pharmacological evaluation of a topical hydrogel containing betamethasone dipropionate microemulsion.					
31176120	1	36	theme	design	314:319	arg1	approach					321:328	design approach	314:328	design approach	314:328	This study was performed to achieve two primary goals: First, a microemulsion containing betamethasone dipropionate was optimized using the quality by design approach.					
31176120	6	37	theme	BMD	1287:1289	arg1	flux					1279:1282	flux	1279:1282	flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent	1279:1373	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	6	37	theme	BMD	1287:1289	arg1	effect					1268:1273	The anti-inflammatory effect	1246:1273	The anti-inflammatory effect	1246:1273	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	6	38	theme	lipid	1489:1493	arg1	NLC-hydrogel					1505:1516	NLC-hydrogel	1505:1516	NLC-hydrogel	1505:1516	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	6	38	theme	lipid	1489:1493	arg1	carriers					1495:1502	nanostructure lipid carriers	1475:1502	nanostructure lipid carriers (NLC-hydrogel)	1475:1517	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	7	39	theme	ME-hydrogel	1620:1630	arg1	NLC-hydrogel					1650:1661	ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel	1620:1661	ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%)	1620:1677	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	7	39	theme	ME-hydrogel	1620:1630	arg1	%					1676:1676	35.93 ± 7.22%	1664:1676	35.93 ± 7.22%	1664:1676	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	3	40	used	used	531:534	arg2	design					520:525	Face-centered central composite design	488:525	Face-centered central composite design	488:525	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	5	41	theme	hydrogel-containing	1197:1215	arg1	ME-hydrogel					1232:1242	ME-hydrogel	1232:1242	ME-hydrogel	1232:1242	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	41	theme	hydrogel-containing	1197:1215	arg1	microemulsion					1217:1229	hydrogel-containing microemulsion	1197:1229	hydrogel-containing microemulsion (ME-hydrogel)	1197:1243	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	42	dep	derivatives	974:984	arg1	NaCMC					1001:1005	NaCMC	1001:1005	NaCMC	1001:1005	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	42	dep	derivatives	974:984	arg1	CMC					1012:1014	CMC	1012:1014	CMC	1012:1014	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	42	dep	derivatives	974:984	arg1	HEC					996:998	HEC	996:998	HEC	996:998	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	42	dep	derivatives	974:984	arg1	derivatives					974:984	Different cellulose derivatives	954:984	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC)	954:1015	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	42	dep	derivatives	974:984	arg1	E6					992:993	HPMC E6	987:993	HPMC E6	987:993	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	7	43	theme	following	1603:1611	arg1	order					1613:1617	the following order	1599:1617	the following order	1599:1617	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	4	44	from	effective	899:907	arg1	terms					912:916	terms	912:916	terms of skin deposition and flux of BMD	912:951	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	3	45	dep	variables	668:676	arg1	size					735:738	microemulsion droplet size	713:738	microemulsion droplet size	713:738	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	45	dep	variables	668:676	arg1	flux					696:699	flux	696:699	flux of BMD	696:706	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	45	dep	variables	668:676	arg1	variables					668:676	three dependent variables	652:676	three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size)	652:739	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	45	dep	variables	668:676	arg1	deposition					684:693	skin deposition	679:693	skin deposition	679:693	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	5	46	theme	microemulsion	1217:1229	arg1	hydration					1137:1145	hydration	1137:1145	hydration of stratum corneum	1137:1164	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	46	theme	microemulsion	1217:1229	arg1	flux					1104:1107	flux	1104:1107	flux	1104:1107	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	46	theme	microemulsion	1217:1229	arg1	deposition					1118:1127	skin deposition	1113:1127	skin deposition	1113:1127	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	46	theme	microemulsion	1217:1229	arg1	properties					1092:1101	four properties	1087:1101	four properties: flux and skin deposition of BMD	1087:1134	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	46	theme	microemulsion	1217:1229	arg1	properties					1183:1192	rheological properties	1171:1192	rheological properties of hydrogel-containing microemulsion (ME-hydrogel)	1171:1243	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	7	47	theme	BMD	1592:1594	arg1	flux					1584:1587	flux	1584:1587	flux of BMD in the following order	1584:1617	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	3	48	theme	skin	679:682	arg1	variables					668:676	three dependent variables	652:676	three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size)	652:739	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	48	theme	skin	679:682	arg1	deposition					684:693	skin deposition	679:693	skin deposition	679:693	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	2	49	dep	irritation	453:462	arg1	skin					448:451	skin	448:451	skin	448:451	Second, a hydrogel-containing microemulsion was developed using cellulose derivatives, and its anti-inflammatory and skin irritation effects were evaluated.					
31176120	1	50	theme	microemulsion	227:239	arg1	dipropionate					266:277	a microemulsion containing betamethasone dipropionate	225:277	a microemulsion containing betamethasone dipropionate	225:277	This study was performed to achieve two primary goals: First, a microemulsion containing betamethasone dipropionate was optimized using the quality by design approach.					
31176120	0	51	theme	pharmacological	61:75	arg1	evaluation					77:86	pharmacological evaluation	61:86	pharmacological evaluation	61:86	DoE-based development, physicochemical characterization, and pharmacological evaluation of a topical hydrogel containing betamethasone dipropionate microemulsion.					
31176120	5	52	theme	skin	1113:1116	arg1	deposition					1118:1127	skin deposition	1113:1127	skin deposition	1113:1127	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	52	theme	skin	1113:1116	arg1	properties					1092:1101	four properties	1087:1101	four properties: flux and skin deposition of BMD	1087:1134	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	4	53	theme	high	827:830	arg1	level					832:836	a high level	825:836	a high level	825:836	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	6	54	theme	hydrogel-forming	1352:1367	arg1	agent					1369:1373	the hydrogel-forming agent	1348:1373	the hydrogel-forming agent	1348:1373	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	6	55	theme	anti-inflammatory	1250:1266	arg1	effect					1268:1273	The anti-inflammatory effect	1246:1273	The anti-inflammatory effect	1246:1273	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	3	56	theme	independent	570:580	arg1	ratio					608:612	ratio	608:612	ratio of surfactant to cosolvent	608:639	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	56	theme	independent	570:580	arg1	variables					582:590	two independent variables	566:590	two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS)	566:647	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	56	theme	independent	570:580	arg1	acid					599:602	oleic acid	593:602	oleic acid	593:602	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	57	dep	acid	599:602	arg1	S/CoS					642:646	S/CoS	642:646	S/CoS	642:646	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	4	58	theme	deposition	926:935	arg1	terms					912:916	terms	912:916	terms of skin deposition and flux of BMD	912:951	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	4	59	from	S/CoS	816:820	arg1	acid					776:779	oleic acid	770:779	oleic acid at a low level (coded with -1) and S/CoS at a high level	770:836	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	6	60	with	ME-hydrogel	1304:1314	arg1	cellulose					1335:1343	carboxymethyl cellulose	1321:1343	carboxymethyl cellulose as the hydrogel-forming agent	1321:1373	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	1	61	theme	containing	241:250	arg1	dipropionate					266:277	a microemulsion containing betamethasone dipropionate	225:277	a microemulsion containing betamethasone dipropionate	225:277	This study was performed to achieve two primary goals: First, a microemulsion containing betamethasone dipropionate was optimized using the quality by design approach.					
31176120	6	62	theme	nanostructure	1475:1487	arg1	NLC-hydrogel					1505:1516	NLC-hydrogel	1505:1516	NLC-hydrogel	1505:1516	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	6	62	theme	nanostructure	1475:1487	arg1	carriers					1495:1502	nanostructure lipid carriers	1475:1502	nanostructure lipid carriers (NLC-hydrogel)	1475:1517	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	5	63	theme	Different	954:962	arg1	NaCMC					1001:1005	NaCMC	1001:1005	NaCMC	1001:1005	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	63	theme	Different	954:962	arg1	CMC					1012:1014	CMC	1012:1014	CMC	1012:1014	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	63	theme	Different	954:962	arg1	HEC					996:998	HEC	996:998	HEC	996:998	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	63	theme	Different	954:962	arg1	derivatives					974:984	Different cellulose derivatives	954:984	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC)	954:1015	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	63	theme	Different	954:962	arg1	E6					992:993	HPMC E6	987:993	HPMC E6	987:993	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	7	64	dep	ME-hydrogel	1620:1630	arg1	%					1645:1645	44.56 ± 8.08%	1633:1645	44.56 ± 8.08%	1633:1645	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	6	65	from	ME-hydrogel	1304:1314	arg1	flux					1279:1282	flux	1279:1282	flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent	1279:1373	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	6	65	from	ME-hydrogel	1304:1314	arg1	effect					1268:1273	The anti-inflammatory effect	1246:1273	The anti-inflammatory effect	1246:1273	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	1	66	theme	betamethasone	252:264	arg1	dipropionate					266:277	a microemulsion containing betamethasone dipropionate	225:277	a microemulsion containing betamethasone dipropionate	225:277	This study was performed to achieve two primary goals: First, a microemulsion containing betamethasone dipropionate was optimized using the quality by design approach.					
31176120	4	67	theme	flux	941:944	arg1	terms					912:916	terms	912:916	terms of skin deposition and flux of BMD	912:951	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	2	68	theme	irritation	453:462	arg1	effects					464:470	its anti-inflammatory and skin irritation effects	422:470	its anti-inflammatory and skin irritation effects	422:470	Second, a hydrogel-containing microemulsion was developed using cellulose derivatives, and its anti-inflammatory and skin irritation effects were evaluated.					
31176120	5	69	theme	cellulose	964:972	arg1	NaCMC					1001:1005	NaCMC	1001:1005	NaCMC	1001:1005	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	69	theme	cellulose	964:972	arg1	CMC					1012:1014	CMC	1012:1014	CMC	1012:1014	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	69	theme	cellulose	964:972	arg1	HEC					996:998	HEC	996:998	HEC	996:998	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	69	theme	cellulose	964:972	arg1	derivatives					974:984	Different cellulose derivatives	954:984	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC)	954:1015	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	5	69	theme	cellulose	964:972	arg1	E6					992:993	HPMC E6	987:993	HPMC E6	987:993	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	6	70	theme	lipid	1436:1440	arg1	SLN-hydrogel					1457:1468	SLN-hydrogel	1457:1468	SLN-hydrogel	1457:1468	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	6	70	theme	lipid	1436:1440	arg1	nanoparticles					1442:1454	the hydrogel-containing solid lipid nanoparticles	1406:1454	the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel)	1406:1469	The anti-inflammatory effect and flux of BMD from optimal ME-hydrogel with carboxymethyl cellulose as the hydrogel-forming agent were then compared to those of the hydrogel-containing solid lipid nanoparticles (SLN-hydrogel) and nanostructure lipid carriers (NLC-hydrogel).					
31176120	3	71	theme	variables	582:590	arg1	impacts					555:561	the impacts	551:561	the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size)	551:739	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	72	theme	composite	510:518	arg1	design					520:525	Face-centered central composite design	488:525	Face-centered central composite design	488:525	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	7	73	theme	inhibition	1544:1553	arg1	percentage					1524:1533	The percentage	1520:1533	The percentage of edema inhibition	1520:1553	The percentage of edema inhibition declined proportionally with flux of BMD in the following order: ME-hydrogel (44.56 ± 8.08%) > NLC-hydrogel (35.93 ± 7.22%) > SLN-hydrogel (25.68 ± 9.05%).					
31176120	5	74	theme	hydrogel-containing	1044:1062	arg1	microemulsion					1064:1076	a hydrogel-containing microemulsion	1042:1076	a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel)	1042:1243	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
31176120	3	75	theme	dependent	658:666	arg1	size					735:738	microemulsion droplet size	713:738	microemulsion droplet size	713:738	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	75	theme	dependent	658:666	arg1	flux					696:699	flux	696:699	flux of BMD	696:706	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	75	theme	dependent	658:666	arg1	variables					668:676	three dependent variables	652:676	three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size)	652:739	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	3	75	theme	dependent	658:666	arg1	deposition					684:693	skin deposition	679:693	skin deposition	679:693	Face-centered central composite design was used to investigate the impacts of two independent variables (oleic acid and ratio of surfactant to cosolvent, S/CoS) on three dependent variables (skin deposition, flux of BMD, and microemulsion droplet size).					
31176120	4	76	theme	skin	921:924	arg1	deposition					926:935	skin deposition	921:935	skin deposition	921:935	The microemulsion including oleic acid at a low level (coded with -1) and S/CoS at a high level (coded with +1) was considered optimal since it was the most effective in terms of skin deposition and flux of BMD.					
31176120	5	77	theme	rheological	1171:1181	arg1	properties					1183:1192	rheological properties	1171:1192	rheological properties of hydrogel-containing microemulsion (ME-hydrogel)	1171:1243	Different cellulose derivatives (HPMC E6, HEC, NaCMC, and CMC) were screened to prepare a hydrogel-containing microemulsion based on four properties: flux and skin deposition of BMD, hydration of stratum corneum, and rheological properties of hydrogel-containing microemulsion (ME-hydrogel).					
30824442	12	0	from	donors	2122:2127	arg1	bacteria					2097:2104	bacteria	2097:2104	bacteria from vegetarian donors	2097:2127	A difference was seen in protein fermentation between omnivore and vegetarian gut microbiotas: bacteria from vegetarian donors grew more on soy and Quorn than on meat and casein, with reduced ammonia production.					
30824442	10	1	theme	levels	1896:1901	arg1	consequences					1867:1878	the negative consequences	1854:1878	the negative consequences of high protein levels	1854:1901	In an in vitro fermentation model, the addition of prebiotics reduced the negative consequences of high protein levels.					
30824442	11	2	theme	metabolites	1976:1986	arg1	reduction					1951:1959	a reduction	1949:1959	a reduction of proteolytic metabolites in the model	1949:1999	Supplementation with a prebiotic resulted in a reduction of proteolytic metabolites in the model.					
30824442	1	3	theme	toxic	262:266	arg1	metabolites					268:278	toxic metabolites	262:278	toxic metabolites	262:278	Metabolism of protein by gut bacteria is potentially detrimental due to the production of toxic metabolites, such as ammonia, amines, p-cresol, and indole.					
30824442	1	3	theme	toxic	262:266	arg1	indole					320:325	indole	320:325	indole	320:325	Metabolism of protein by gut bacteria is potentially detrimental due to the production of toxic metabolites, such as ammonia, amines, p-cresol, and indole.					
30824442	1	3	theme	toxic	262:266	arg1	amines					298:303	amines	298:303	amines	298:303	Metabolism of protein by gut bacteria is potentially detrimental due to the production of toxic metabolites, such as ammonia, amines, p-cresol, and indole.					
30824442	1	3	theme	toxic	262:266	arg1	p-cresol					306:313	p-cresol	306:313	p-cresol	306:313	Metabolism of protein by gut bacteria is potentially detrimental due to the production of toxic metabolites, such as ammonia, amines, p-cresol, and indole.					
30824442	1	3	theme	toxic	262:266	arg1	ammonia					289:295	ammonia	289:295	ammonia	289:295	Metabolism of protein by gut bacteria is potentially detrimental due to the production of toxic metabolites, such as ammonia, amines, p-cresol, and indole.					
30824442	9	4	from	proteolysis	1771:1781	arg1	metabolites					1743:1753	potentially harmful metabolites	1723:1753	potentially harmful metabolites in the gut from proteolysis	1723:1781	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	9	4	from	proteolysis	1771:1781	arg1	gut					1762:1764	the gut	1758:1764	the gut from proteolysis	1758:1781	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	10	5	theme	high	1883:1886	arg1	levels					1896:1901	high protein levels	1883:1901	high protein levels	1883:1901	In an in vitro fermentation model, the addition of prebiotics reduced the negative consequences of high protein levels.					
30824442	13	6	from	donors	2239:2244	arg1	Bacteria					2214:2221	Bacteria	2214:2221	Bacteria from vegetarian donors	2214:2244	Bacteria from vegetarian donors produced less branched-chain fatty acids (BCFA).					
30824442	9	7	theme	compounds.IMPORTANCE	1625:1644	arg1	reduction					1606:1614	a reduction	1604:1614	a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis	1604:1781	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	10	8	dep	in	1790:1791	arg1	vitro					1793:1797	vitro	1793:1797	vitro	1793:1797	In an in vitro fermentation model, the addition of prebiotics reduced the negative consequences of high protein levels.					
30824442	6	9	theme	prebiotic	1266:1274	arg1	treatment					1276:1284	prebiotic treatment	1266:1284	prebiotic treatment	1266:1284	Branched-chain fatty acids (BCFA) were significantly lower in fermenters with vegetarians' feces (P = 0.004), reduced further by prebiotic treatment.					
30824442	1	10	theme	protein	186:192	arg1	Metabolism					172:181	Metabolism	172:181	Metabolism of protein by gut bacteria	172:208	Metabolism of protein by gut bacteria is potentially detrimental due to the production of toxic metabolites, such as ammonia, amines, p-cresol, and indole.					
30824442	9	11	theme	protein	1654:1660	arg1	intake					1662:1667	Dietary protein intake	1646:1667	Dietary protein intake	1646:1667	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	1	12	theme	gut	197:199	arg1	bacteria					201:208	gut bacteria	197:208	gut bacteria	197:208	Metabolism of protein by gut bacteria is potentially detrimental due to the production of toxic metabolites, such as ammonia, amines, p-cresol, and indole.					
30824442	4	13	theme	short-chain	880:890	arg1	indole					929:934	indole	929:934	indole	929:934	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	13	theme	short-chain	880:890	arg1	ammonia					912:918	ammonia	912:918	ammonia	912:918	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	13	theme	short-chain	880:890	arg1	p-cresol					941:948	p-cresol	941:948	p-cresol	941:948	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	13	theme	short-chain	880:890	arg1	acids					898:902	short-chain fatty acids	880:902	short-chain fatty acids (SCFA)	880:909	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	13	theme	short-chain	880:890	arg1	phenol					921:926	phenol	921:926	phenol	921:926	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	13	theme	short-chain	880:890	arg1	SCFA					905:908	SCFA	905:908	SCFA	905:908	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	12	14	theme	gut	2080:2082	arg1	microbiotas					2084:2094	vegetarian gut microbiotas	2069:2094	vegetarian gut microbiotas	2069:2094	A difference was seen in protein fermentation between omnivore and vegetarian gut microbiotas: bacteria from vegetarian donors grew more on soy and Quorn than on meat and casein, with reduced ammonia production.					
30824442	3	15	from	omnivores	659:667	arg1	mycoprotein					741:751	mycoprotein	741:751	mycoprotein	741:751	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	15	from	omnivores	659:667	arg1	sources					718:724	four protein sources	705:724	four protein sources (casein, meat, mycoprotein, and soy protein)	705:769	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	15	from	omnivores	659:667	arg1	casein					727:732	casein	727:732	casein	727:732	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	15	from	omnivores	659:667	arg1	protein					762:768	soy protein	758:768	soy protein	758:768	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	15	from	omnivores	659:667	arg1	meat					735:738	meat	735:738	meat	735:738	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	15	from	omnivores	659:667	arg1	feces					648:652	feces	648:652	feces from omnivores (n = 3) and vegetarians (n = 3)	648:699	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	0	16	theme	Bacterial	121:129	arg1	Metabolism					131:140	Gut Bacterial Metabolism	117:140	Gut Bacterial Metabolism	117:140	Prebiotic Supplementation of In Vitro Fecal Fermentations Inhibits Proteolysis by Gut Bacteria, and Host Diet Shapes Gut Bacterial Metabolism and Response to Intervention.					
30824442	5	17	theme	fructan	1002:1008	arg1	Synergy1					1020:1027	the fructan prebiotic Synergy1	998:1027	the fructan prebiotic Synergy1	998:1027	Addition of the fructan prebiotic Synergy1 increased levels of bifidobacteria (P = 0.000019 and 0.000013 for omnivores and vegetarians, respectively).					
30824442	12	18	theme	protein	2027:2033	arg1	fermentation					2035:2046	protein fermentation	2027:2046	protein fermentation between omnivore and vegetarian gut microbiotas	2027:2094	A difference was seen in protein fermentation between omnivore and vegetarian gut microbiotas: bacteria from vegetarian donors grew more on soy and Quorn than on meat and casein, with reduced ammonia production.					
30824442	3	19	theme	protein	710:716	arg1	sources					718:724	four protein sources	705:724	four protein sources (casein, meat, mycoprotein, and soy protein)	705:769	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	19	theme	protein	710:716	arg1	casein					727:732	casein	727:732	casein	727:732	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	19	theme	protein	710:716	arg1	meat					735:738	meat	735:738	meat	735:738	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	19	theme	protein	710:716	arg1	mycoprotein					741:751	mycoprotein	741:751	mycoprotein	741:751	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	19	theme	protein	710:716	arg1	protein					762:768	soy protein	758:768	soy protein	758:768	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	13	20	theme	vegetarian	2228:2237	arg1	donors					2239:2244	vegetarian donors	2228:2244	vegetarian donors	2228:2244	Bacteria from vegetarian donors produced less branched-chain fatty acids (BCFA).					
30824442	7	21	theme	Ammonia	1287:1293	arg1	production					1295:1304	Ammonia production	1287:1304	Ammonia production	1287:1304	Ammonia production was lower with Synergy1.					
30824442	3	22	theme	vitro	588:592	arg1	cultures					618:625	vitro Anaerobic stirred batch cultures	588:625	vitro Anaerobic stirred batch cultures	588:625	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	8	23	theme	ammonia	1432:1438	arg1	production					1440:1449	ammonia production	1432:1449	ammonia production between vegetarians and omnivores	1432:1483	Bacterial adaptation to different dietary protein sources was observed through different patterns of ammonia production between vegetarians and omnivores.					
30824442	6	24	theme	Branched-chain	1137:1150	arg1	BCFA					1165:1168	BCFA	1165:1168	BCFA	1165:1168	Branched-chain fatty acids (BCFA) were significantly lower in fermenters with vegetarians' feces (P = 0.004), reduced further by prebiotic treatment.					
30824442	6	24	theme	Branched-chain	1137:1150	arg1	acids					1158:1162	Branched-chain fatty acids	1137:1162	Branched-chain fatty acids (BCFA)	1137:1169	Branched-chain fatty acids (BCFA) were significantly lower in fermenters with vegetarians' feces (P = 0.004), reduced further by prebiotic treatment.					
30824442	5	25	theme	bifidobacteria	1049:1062	arg1	levels					1039:1044	levels	1039:1044	levels of bifidobacteria (P = 0.000019 and 0.000013 for omnivores and vegetarians, respectively)	1039:1134	Addition of the fructan prebiotic Synergy1 increased levels of bifidobacteria (P = 0.000019 and 0.000013 for omnivores and vegetarians, respectively).					
30824442	3	26	theme	soy	758:760	arg1	sources					718:724	four protein sources	705:724	four protein sources (casein, meat, mycoprotein, and soy protein)	705:769	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	26	theme	soy	758:760	arg1	protein					762:768	soy protein	758:768	soy protein	758:768	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	0	27	theme	Gut	82:84	arg1	Bacteria					86:93	Gut Bacteria	82:93	Gut Bacteria	82:93	Prebiotic Supplementation of In Vitro Fecal Fermentations Inhibits Proteolysis by Gut Bacteria, and Host Diet Shapes Gut Bacterial Metabolism and Response to Intervention.					
30824442	9	28	from	high	1672:1675	arg1	populations					1688:1698	Western populations	1680:1698	Western populations	1680:1698	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	4	29	theme	acids	898:902	arg1	concentrations					862:875	concentrations	862:875	concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol	862:948	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	29	theme	acids	898:902	arg1	counts					851:856	Bacterial counts	841:856	Bacterial counts	841:856	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	11	30	with	Supplementation	1904:1918	arg1	prebiotic					1927:1935	a prebiotic	1925:1935	a prebiotic	1925:1935	Supplementation with a prebiotic resulted in a reduction of proteolytic metabolites in the model.					
30824442	5	31	theme	Synergy1	1020:1027	arg1	Addition					986:993	Addition	986:993	Addition of the fructan prebiotic Synergy1	986:1027	Addition of the fructan prebiotic Synergy1 increased levels of bifidobacteria (P = 0.000019 and 0.000013 for omnivores and vegetarians, respectively).					
30824442	9	32	theme	high	1512:1515	arg1	levels					1526:1531	high baseline levels	1512:1531	high baseline levels of phenol, indole, p-cresol, and skatole	1512:1572	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	12	33	theme	ammonia	2194:2200	arg1	production					2202:2211	reduced ammonia production	2186:2211	reduced ammonia production	2186:2211	A difference was seen in protein fermentation between omnivore and vegetarian gut microbiotas: bacteria from vegetarian donors grew more on soy and Quorn than on meat and casein, with reduced ammonia production.					
30824442	12	34	theme	reduced	2186:2192	arg1	production					2202:2211	reduced ammonia production	2186:2211	reduced ammonia production	2186:2211	A difference was seen in protein fermentation between omnivore and vegetarian gut microbiotas: bacteria from vegetarian donors grew more on soy and Quorn than on meat and casein, with reduced ammonia production.					
30824442	0	35	theme	Host	100:103	arg1	Diet					105:108	Host Diet	100:108	Host Diet	100:108	Prebiotic Supplementation of In Vitro Fecal Fermentations Inhibits Proteolysis by Gut Bacteria, and Host Diet Shapes Gut Bacterial Metabolism and Response to Intervention.					
30824442	2	36	dep	composition	406:416	arg1	the					402:404	the	402:404	the	402:404	The consumption of prebiotic carbohydrates results in specific changes in the composition and/or activity of the microbiota that may confer benefits to host well-being and health.					
30824442	2	37	theme	carbohydrates	357:369	arg1	consumption					332:342	The consumption	328:342	The consumption of prebiotic carbohydrates	328:369	The consumption of prebiotic carbohydrates results in specific changes in the composition and/or activity of the microbiota that may confer benefits to host well-being and health.					
30824442	0	38	theme	Prebiotic	0:8	arg1	Supplementation					10:24	Prebiotic Supplementation	0:24	Prebiotic Supplementation of In Vitro Fecal Fermentations	0:56	Prebiotic Supplementation of In Vitro Fecal Fermentations Inhibits Proteolysis by Gut Bacteria, and Host Diet Shapes Gut Bacterial Metabolism and Response to Intervention.					
30824442	1	39	theme	metabolites	268:278	arg1	production					248:257	the production	244:257	the production of toxic metabolites, such as ammonia, amines, p-cresol, and indole	244:325	Metabolism of protein by gut bacteria is potentially detrimental due to the production of toxic metabolites, such as ammonia, amines, p-cresol, and indole.					
30824442	9	40	theme	phenol	1536:1541	arg1	levels					1526:1531	high baseline levels	1512:1531	high baseline levels of phenol, indole, p-cresol, and skatole	1512:1572	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	9	41	from	metabolites	1743:1753	arg1	proteolysis					1771:1781	proteolysis	1771:1781	proteolysis	1771:1781	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	9	41	from	metabolites	1743:1753	arg1	gut					1762:1764	the gut	1758:1764	the gut from proteolysis	1758:1781	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	9	42	theme	indole	1544:1549	arg1	levels					1526:1531	high baseline levels	1512:1531	high baseline levels of phenol, indole, p-cresol, and skatole	1512:1572	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	8	43	theme	Bacterial	1331:1339	arg1	adaptation					1341:1350	Bacterial adaptation	1331:1350	Bacterial adaptation to different dietary protein sources	1331:1387	Bacterial adaptation to different dietary protein sources was observed through different patterns of ammonia production between vegetarians and omnivores.					
30824442	9	44	theme	p-cresol	1552:1559	arg1	levels					1526:1531	high baseline levels	1512:1531	high baseline levels of phenol, indole, p-cresol, and skatole	1512:1572	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	2	45	theme	microbiota	441:450	arg1	composition					406:416	composition	406:416	composition	406:416	The consumption of prebiotic carbohydrates results in specific changes in the composition and/or activity of the microbiota that may confer benefits to host well-being and health.					
30824442	3	46	theme	stirred	604:610	arg1	cultures					618:625	vitro Anaerobic stirred batch cultures	588:625	vitro Anaerobic stirred batch cultures	588:625	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	0	47	theme	Fermentations	44:56	arg1	Supplementation					10:24	Prebiotic Supplementation	0:24	Prebiotic Supplementation of In Vitro Fecal Fermentations	0:56	Prebiotic Supplementation of In Vitro Fecal Fermentations Inhibits Proteolysis by Gut Bacteria, and Host Diet Shapes Gut Bacterial Metabolism and Response to Intervention.					
30824442	3	48	theme	Anaerobic	594:602	arg1	cultures					618:625	vitro Anaerobic stirred batch cultures	588:625	vitro Anaerobic stirred batch cultures	588:625	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	8	49	theme	dietary	1365:1371	arg1	sources					1381:1387	different dietary protein sources	1355:1387	different dietary protein sources	1355:1387	Bacterial adaptation to different dietary protein sources was observed through different patterns of ammonia production between vegetarians and omnivores.					
30824442	2	50	from	changes	391:397	arg1	activity					425:432	activity	425:432	activity	425:432	The consumption of prebiotic carbohydrates results in specific changes in the composition and/or activity of the microbiota that may confer benefits to host well-being and health.					
30824442	2	50	from	changes	391:397	arg1	composition					406:416	composition	406:416	composition	406:416	The consumption of prebiotic carbohydrates results in specific changes in the composition and/or activity of the microbiota that may confer benefits to host well-being and health.					
30824442	11	51	from	reduction	1951:1959	arg1	model					1995:1999	the model	1991:1999	the model	1991:1999	Supplementation with a prebiotic resulted in a reduction of proteolytic metabolites in the model.					
30824442	10	52	theme	negative	1858:1865	arg1	consequences					1867:1878	the negative consequences	1854:1878	the negative consequences of high protein levels	1854:1901	In an in vitro fermentation model, the addition of prebiotics reduced the negative consequences of high protein levels.					
30824442	11	53	theme	proteolytic	1964:1974	arg1	metabolites					1976:1986	proteolytic metabolites	1964:1986	proteolytic metabolites	1964:1986	Supplementation with a prebiotic resulted in a reduction of proteolytic metabolites in the model.					
30824442	10	54	theme	protein	1888:1894	arg1	levels					1896:1901	high protein levels	1883:1901	high protein levels	1883:1901	In an in vitro fermentation model, the addition of prebiotics reduced the negative consequences of high protein levels.					
30824442	9	55	theme	Dietary	1646:1652	arg1	intake					1662:1667	Dietary protein intake	1646:1667	Dietary protein intake	1646:1667	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	3	56	from	vegetarians	681:691	arg1	mycoprotein					741:751	mycoprotein	741:751	mycoprotein	741:751	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	56	from	vegetarians	681:691	arg1	sources					718:724	four protein sources	705:724	four protein sources (casein, meat, mycoprotein, and soy protein)	705:769	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	56	from	vegetarians	681:691	arg1	casein					727:732	casein	727:732	casein	727:732	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	56	from	vegetarians	681:691	arg1	protein					762:768	soy protein	758:768	soy protein	758:768	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	56	from	vegetarians	681:691	arg1	meat					735:738	meat	735:738	meat	735:738	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	56	from	vegetarians	681:691	arg1	feces					648:652	feces	648:652	feces from omnivores (n = 3) and vegetarians (n = 3)	648:699	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	0	57	theme	Gut	117:119	arg1	Metabolism					131:140	Gut Bacterial Metabolism	117:140	Gut Bacterial Metabolism	117:140	Prebiotic Supplementation of In Vitro Fecal Fermentations Inhibits Proteolysis by Gut Bacteria, and Host Diet Shapes Gut Bacterial Metabolism and Response to Intervention.					
30824442	13	58	theme	fatty	2275:2279	arg1	BCFA					2288:2291	BCFA	2288:2291	BCFA	2288:2291	Bacteria from vegetarian donors produced less branched-chain fatty acids (BCFA).					
30824442	13	58	theme	fatty	2275:2279	arg1	acids					2281:2285	branched-chain fatty acids	2260:2285	branched-chain fatty acids (BCFA)	2260:2292	Bacteria from vegetarian donors produced less branched-chain fatty acids (BCFA).					
30824442	6	59	dep	reduced	1247:1253	arg1	P = 0.004					1235:1243	P = 0.004	1235:1243	P = 0.004	1235:1243	Branched-chain fatty acids (BCFA) were significantly lower in fermenters with vegetarians' feces (P = 0.004), reduced further by prebiotic treatment.					
30824442	8	60	theme	different	1410:1418	arg1	patterns					1420:1427	different patterns	1410:1427	different patterns of ammonia production between vegetarians and omnivores	1410:1483	Bacterial adaptation to different dietary protein sources was observed through different patterns of ammonia production between vegetarians and omnivores.					
30824442	12	61	theme	vegetarian	2069:2078	arg1	microbiotas					2084:2094	vegetarian gut microbiotas	2069:2094	vegetarian gut microbiotas	2069:2094	A difference was seen in protein fermentation between omnivore and vegetarian gut microbiotas: bacteria from vegetarian donors grew more on soy and Quorn than on meat and casein, with reduced ammonia production.					
30824442	5	62	theme	prebiotic	1010:1018	arg1	Synergy1					1020:1027	the fructan prebiotic Synergy1	998:1027	the fructan prebiotic Synergy1	998:1027	Addition of the fructan prebiotic Synergy1 increased levels of bifidobacteria (P = 0.000019 and 0.000013 for omnivores and vegetarians, respectively).					
30824442	3	63	theme	prebiotics	544:553	arg1	impact					534:539	the impact	530:539	the impact of prebiotics on proteolysis within the gut	530:583	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	9	64	theme	Western	1680:1686	arg1	populations					1688:1698	Western populations	1680:1698	Western populations	1680:1698	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	0	65	dep	In	29:30	arg1	Vitro					32:36	Vitro	32:36	Vitro	32:36	Prebiotic Supplementation of In Vitro Fecal Fermentations Inhibits Proteolysis by Gut Bacteria, and Host Diet Shapes Gut Bacterial Metabolism and Response to Intervention.					
30824442	10	66	theme	in	1790:1791	arg1	model					1812:1816	an in vitro fermentation model	1787:1816	an in vitro fermentation model	1787:1816	In an in vitro fermentation model, the addition of prebiotics reduced the negative consequences of high protein levels.					
30824442	0	67	theme	Fecal	38:42	arg1	Fermentations					44:56	In Vitro Fecal Fermentations	29:56	In Vitro Fecal Fermentations	29:56	Prebiotic Supplementation of In Vitro Fecal Fermentations Inhibits Proteolysis by Gut Bacteria, and Host Diet Shapes Gut Bacterial Metabolism and Response to Intervention.					
30824442	9	68	from	populations	1688:1698	arg1	high					1672:1675	high	1672:1675	high	1672:1675	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	4	69	theme	Bacterial	841:849	arg1	counts					851:856	Bacterial counts	841:856	Bacterial counts	841:856	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	8	70	theme	production	1440:1449	arg1	patterns					1420:1427	different patterns	1410:1427	different patterns of ammonia production between vegetarians and omnivores	1410:1483	Bacterial adaptation to different dietary protein sources was observed through different patterns of ammonia production between vegetarians and omnivores.					
30824442	6	71	theme	fatty	1152:1156	arg1	BCFA					1165:1168	BCFA	1165:1168	BCFA	1165:1168	Branched-chain fatty acids (BCFA) were significantly lower in fermenters with vegetarians' feces (P = 0.004), reduced further by prebiotic treatment.					
30824442	6	71	theme	fatty	1152:1156	arg1	acids					1158:1162	Branched-chain fatty acids	1137:1162	Branched-chain fatty acids (BCFA)	1137:1169	Branched-chain fatty acids (BCFA) were significantly lower in fermenters with vegetarians' feces (P = 0.004), reduced further by prebiotic treatment.					
30824442	3	72	from	impact	534:539	arg1	proteolysis					558:568	proteolysis	558:568	proteolysis within the gut	558:583	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	9	73	theme	harmful	1735:1741	arg1	metabolites					1743:1753	potentially harmful metabolites	1723:1753	potentially harmful metabolites in the gut from proteolysis	1723:1781	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	9	74	theme	volunteer	1489:1497	arg1	samples					1499:1505	volunteer samples	1489:1505	volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole	1489:1572	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	3	75	theme	oligofructose-enriched	810:831	arg1	inulin					833:838	an oligofructose-enriched inulin	807:838	an oligofructose-enriched inulin	807:838	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	9	76	with	samples	1499:1505	arg1	levels					1526:1531	high baseline levels	1512:1531	high baseline levels of phenol, indole, p-cresol, and skatole	1512:1572	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	12	77	theme	vegetarian	2111:2120	arg1	donors					2122:2127	vegetarian donors	2111:2127	vegetarian donors	2111:2127	A difference was seen in protein fermentation between omnivore and vegetarian gut microbiotas: bacteria from vegetarian donors grew more on soy and Quorn than on meat and casein, with reduced ammonia production.					
30824442	4	78	theme	fatty	892:896	arg1	indole					929:934	indole	929:934	indole	929:934	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	78	theme	fatty	892:896	arg1	ammonia					912:918	ammonia	912:918	ammonia	912:918	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	78	theme	fatty	892:896	arg1	p-cresol					941:948	p-cresol	941:948	p-cresol	941:948	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	78	theme	fatty	892:896	arg1	acids					898:902	short-chain fatty acids	880:902	short-chain fatty acids (SCFA)	880:909	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	78	theme	fatty	892:896	arg1	phenol					921:926	phenol	921:926	phenol	921:926	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	4	78	theme	fatty	892:896	arg1	SCFA					905:908	SCFA	905:908	SCFA	905:908	Bacterial counts and concentrations of short-chain fatty acids (SCFA), ammonia, phenol, indole, and p-cresol were monitored during fermentation.					
30824442	3	79	dep	sources	718:724	arg1	sources					718:724	four protein sources	705:724	four protein sources (casein, meat, mycoprotein, and soy protein)	705:769	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	79	dep	sources	718:724	arg1	casein					727:732	casein	727:732	casein	727:732	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	79	dep	sources	718:724	arg1	meat					735:738	meat	735:738	meat	735:738	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	79	dep	sources	718:724	arg1	mycoprotein					741:751	mycoprotein	741:751	mycoprotein	741:751	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	3	79	dep	sources	718:724	arg1	protein					762:768	soy protein	758:768	soy protein	758:768	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	13	80	theme	branched-chain	2260:2273	arg1	BCFA					2288:2291	BCFA	2288:2291	BCFA	2288:2291	Bacteria from vegetarian donors produced less branched-chain fatty acids (BCFA).					
30824442	13	80	theme	branched-chain	2260:2273	arg1	acids					2281:2285	branched-chain fatty acids	2260:2285	branched-chain fatty acids (BCFA)	2260:2292	Bacteria from vegetarian donors produced less branched-chain fatty acids (BCFA).					
30824442	9	81	theme	baseline	1517:1524	arg1	levels					1526:1531	high baseline levels	1512:1531	high baseline levels of phenol, indole, p-cresol, and skatole	1512:1572	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	2	82	theme	prebiotic	347:355	arg1	carbohydrates					357:369	prebiotic carbohydrates	347:369	prebiotic carbohydrates	347:369	The consumption of prebiotic carbohydrates results in specific changes in the composition and/or activity of the microbiota that may confer benefits to host well-being and health.					
30824442	0	83	theme	In	29:30	arg1	Fermentations					44:56	In Vitro Fecal Fermentations	29:56	In Vitro Fecal Fermentations	29:56	Prebiotic Supplementation of In Vitro Fecal Fermentations Inhibits Proteolysis by Gut Bacteria, and Host Diet Shapes Gut Bacterial Metabolism and Response to Intervention.					
30824442	5	84	dep	bifidobacteria	1049:1062	arg1	0.000013					1082:1089	0.000013	1082:1089	0.000013	1082:1089	Addition of the fructan prebiotic Synergy1 increased levels of bifidobacteria (P = 0.000019 and 0.000013 for omnivores and vegetarians, respectively).					
30824442	5	84	dep	bifidobacteria	1049:1062	arg1	P = 0.000019					1065:1076	P = 0.000019	1065:1076	P = 0.000019	1065:1076	Addition of the fructan prebiotic Synergy1 increased levels of bifidobacteria (P = 0.000019 and 0.000013 for omnivores and vegetarians, respectively).					
30824442	3	85	theme	batch	612:616	arg1	cultures					618:625	vitro Anaerobic stirred batch cultures	588:625	vitro Anaerobic stirred batch cultures	588:625	Here, we have studied the impact of prebiotics on proteolysis within the gut in vitro Anaerobic stirred batch cultures were inoculated with feces from omnivores (n = 3) and vegetarians (n = 3) and four protein sources (casein, meat, mycoprotein, and soy protein) with and without supplementation by an oligofructose-enriched inulin.					
30824442	9	86	dep	compounds.IMPORTANCE	1625:1644	arg1	high					1672:1675	high	1672:1675	high	1672:1675	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	10	87	theme	fermentation	1799:1810	arg1	model					1812:1816	an in vitro fermentation model	1787:1816	an in vitro fermentation model	1787:1816	In an in vitro fermentation model, the addition of prebiotics reduced the negative consequences of high protein levels.					
30824442	2	88	theme	specific	382:389	arg1	changes					391:397	specific changes	382:397	specific changes in the composition and/or activity of the microbiota that may confer benefits to host well-being and health	382:505	The consumption of prebiotic carbohydrates results in specific changes in the composition and/or activity of the microbiota that may confer benefits to host well-being and health.					
30824442	10	89	theme	prebiotics	1835:1844	arg1	addition					1823:1830	the addition	1819:1830	the addition of prebiotics	1819:1844	In an in vitro fermentation model, the addition of prebiotics reduced the negative consequences of high protein levels.					
30824442	2	90	dep	host	480:483	arg1	well-being					485:494	well-being	485:494	well-being	485:494	The consumption of prebiotic carbohydrates results in specific changes in the composition and/or activity of the microbiota that may confer benefits to host well-being and health.					
30824442	9	91	theme	skatole	1566:1572	arg1	levels					1526:1531	high baseline levels	1512:1531	high baseline levels of phenol, indole, p-cresol, and skatole	1512:1572	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	8	92	theme	different	1355:1363	arg1	sources					1381:1387	different dietary protein sources	1355:1387	different dietary protein sources	1355:1387	Bacterial adaptation to different dietary protein sources was observed through different patterns of ammonia production between vegetarians and omnivores.					
30824442	9	93	theme	Synergy1	1575:1582	arg1	fermentation					1584:1595	Synergy1 fermentation	1575:1595	Synergy1 fermentation	1575:1595	In volunteer samples with high baseline levels of phenol, indole, p-cresol, and skatole, Synergy1 fermentation led to a reduction of these compounds.IMPORTANCE Dietary protein intake is high in Western populations, which could result in potentially harmful metabolites in the gut from proteolysis.					
30824442	8	94	theme	protein	1373:1379	arg1	sources					1381:1387	different dietary protein sources	1355:1387	different dietary protein sources	1355:1387	Bacterial adaptation to different dietary protein sources was observed through different patterns of ammonia production between vegetarians and omnivores.					
30690616	6	0	theme	final	960:964	arg1	BW					966:967	final BW	960:967	final BW	960:967	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	10	1	theme	C	1702:1702	arg1	groups					1713:1718	C and SYN2 groups	1702:1718	C and SYN2 groups	1702:1718	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	8	2	theme	acids	1538:1542	arg1	PUFA					1556:1559	n-6 PUFA	1552:1559	n-6 PUFA	1552:1559	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	2	theme	acids	1538:1542	arg1	PUFA					1569:1572	n-3 PUFA	1565:1572	n-3 PUFA	1565:1572	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	2	theme	acids	1538:1542	arg1	content					1374:1380	a higher (P < 0.01) content	1354:1380	a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA)	1354:1549	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	3	theme	fatty	1436:1440	arg1	acids					1442:1446	lower monounsaturated fatty acids	1414:1446	lower monounsaturated fatty acids (P < 0.05 compared only to SYN2)	1414:1479	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	1	4	theme	meat	224:227	arg1	traits					237:242	slaughter performance and meat quality traits	198:242	traits	237:242	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	2	5	dep	formulations	463:474	arg1	SYN1					477:480	SYN1	477:480	SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides	477:599	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	11	6	theme	slaughter	1950:1958	arg1	performance					1960:1970	slaughter performance	1950:1970	slaughter performance	1950:1970	In conclusion, this study has shown that in ovo administration of synbiotics did not negatively affect slaughter performance and physicochemical properties of meat.					
30690616	12	7	theme	fatty	2068:2072	arg1	profile					2079:2085	a preferable fatty acid profile	2055:2085	a preferable fatty acid profile	2055:2085	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	8	8	theme	fatty	1395:1399	arg1	SFA					1408:1410	SFA	1408:1410	SFA	1408:1410	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	8	theme	fatty	1395:1399	arg1	acids					1401:1405	saturated fatty acids	1385:1405	saturated fatty acids (SFA)	1385:1411	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	3	9	theme	chicks	654:659	arg1	group					665:669	80 chicks per group	651:669	80 chicks per group	651:669	After hatching, 240 males were randomly chosen (80 chicks per group) and split into 8 replicate pens (10 birds per pen).					
30690616	2	10	dep	SYN1	477:480	arg1	SYN2					535:538	SYN2	535:538	SYN2	535:538	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	2	10	dep	SYN1	477:480	arg1	plantarum					555:563	Lactobacillus plantarum	541:563	Lactobacillus plantarum	541:563	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	2	10	dep	SYN1	477:480	arg1	galactooligosaccharides					510:532	galactooligosaccharides	510:532	galactooligosaccharides	510:532	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	2	10	dep	SYN1	477:480	arg1	salivarius					497:506	Lactobacillus salivarius	483:506	Lactobacillus salivarius	483:506	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	2	10	dep	SYN1	477:480	arg1	oligosaccharides					584:599	raffinose family oligosaccharides	567:599	raffinose family oligosaccharides	567:599	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	7	11	theme	C	1273:1273	arg1	group					1275:1279	C group	1273:1279	C group	1273:1279	Synbiotic administration reduced (P = 0.061) the lipid content compared with C group, markedly (P < 0.05) with synbiotic SYN2.					
30690616	2	12	theme	raffinose	567:575	arg1	oligosaccharides					584:599	raffinose family oligosaccharides	567:599	raffinose family oligosaccharides	567:599	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	6	13	theme	water	1074:1078	arg1	pH					1063:1064	pH	1063:1064	pH	1063:1064	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	13	theme	water	1074:1078	arg1	capacity					1088:1095	water holding capacity	1074:1095	water holding capacity	1074:1095	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	4	14	theme	age	735:737	arg1	d					730:730	42 d	727:730	42 d of age	727:737	At 42 d of age, 15 birds per treatment were weighed and slaughtered.					
30690616	6	15	theme	pectoral	1003:1010	arg1	PM					1020:1021	PM	1020:1021	PM	1020:1021	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	15	theme	pectoral	1003:1010	arg1	muscle					1012:1017	pectoral muscle	1003:1017	pectoral muscle (PM)	1003:1022	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	10	16	theme	SYN2	1708:1711	arg1	groups					1713:1718	C and SYN2 groups	1702:1718	C and SYN2 groups	1702:1718	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	11	17	dep	in	1888:1889	arg1	ovo					1891:1893	ovo	1891:1893	ovo	1891:1893	In conclusion, this study has shown that in ovo administration of synbiotics did not negatively affect slaughter performance and physicochemical properties of meat.					
30690616	6	18	theme	carcass	991:997	arg1	BW					966:967	final BW	960:967	final BW	960:967	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	18	theme	carcass	991:997	arg1	weight					970:975	weight	970:975	weight	970:975	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	18	theme	carcass	991:997	arg1	yield					982:986	yield	982:986	yield	982:986	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	5	19	contain	had	825:827	arg2	effect					835:840	a low effect	829:840	a low effect	829:840	In ovo synbiotic administration had a low effect on investigated traits, but depends on the kind of synbiotic administered.					
30690616	5	19	contain	had	825:827	arg1	administration					810:823	synbiotic administration	800:823	synbiotic administration	800:823	In ovo synbiotic administration had a low effect on investigated traits, but depends on the kind of synbiotic administered.					
30690616	8	20	theme	n-3	1565:1567	arg1	PUFA					1569:1572	n-3 PUFA	1565:1572	n-3 PUFA	1565:1572	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	1	21	theme	in	163:164	arg1	administration					180:193	in ovo synbiotic administration	163:193	in ovo synbiotic administration	163:193	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	7	22	theme	Synbiotic	1196:1204	arg1	administration					1206:1219	Synbiotic administration	1196:1219	Synbiotic administration	1196:1219	Synbiotic administration reduced (P = 0.061) the lipid content compared with C group, markedly (P < 0.05) with synbiotic SYN2.					
30690616	10	23	theme	higher	1732:1737	arg1	ratio					1750:1754	a higher (P < 0.01) ratio	1730:1754	a higher (P < 0.01) ratio of PUFA to SFA	1730:1769	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	6	24	theme	cholesterol	1138:1148	arg1	content					1150:1156	cholesterol content	1138:1156	cholesterol content of PM	1138:1162	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	1	25	theme	synbiotic	170:178	arg1	administration					180:193	in ovo synbiotic administration	163:193	in ovo synbiotic administration	163:193	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	5	26	theme	synbiotic	893:901	arg1	kind					885:888	the kind	881:888	the kind of synbiotic administered	881:914	In ovo synbiotic administration had a low effect on investigated traits, but depends on the kind of synbiotic administered.					
30690616	9	27	theme	P	1680:1680	arg1	administration					1664:1677	the synbiotic administration	1650:1677	the synbiotic administration (P = 0.039)	1650:1689	The ratio of n-6 to n-3 PUFA was affected by the synbiotic administration (P = 0.039).					
30690616	9	27	theme	P	1680:1680	arg1	0.039					1684:1688	P = 0.039	1680:1688	P = 0.039	1680:1688	The ratio of n-6 to n-3 PUFA was affected by the synbiotic administration (P = 0.039).					
30690616	6	28	theme	physicochemical	1035:1049	arg1	properties					1051:1060	physicochemical properties	1035:1060	physicochemical properties (pH, color, water holding capacity)	1035:1096	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	8	29	theme	SYN2	1592:1595	arg1	groups					1597:1602	SYN2 groups	1592:1602	SYN2 groups	1592:1602	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	1	30	from	effect	153:158	arg1	performance					208:218	slaughter performance and meat quality traits	198:242	performance	208:218	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	1	30	from	effect	153:158	arg1	traits					237:242	slaughter performance and meat quality traits	198:242	traits	237:242	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	1	31	theme	chickens	255:262	arg1	performance					208:218	slaughter performance and meat quality traits	198:242	performance	208:218	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	1	31	theme	chickens	255:262	arg1	traits					237:242	slaughter performance and meat quality traits	198:242	traits	237:242	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	6	32	theme	collagen	1113:1120	arg1	properties					1122:1131	intramuscular collagen properties	1099:1131	intramuscular collagen properties	1099:1131	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	12	33	theme	chicken	2140:2146	arg1	meat					2148:2151	chicken meat	2140:2151	chicken meat	2140:2151	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	2	34	theme	different	443:451	arg1	formulations					463:474	2 different synbiotic formulations	441:474	2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides)	441:600	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	0	35	from	Effect	0:5	arg1	meat					71:74	meat	71:74	meat	71:74	Effect of in ovo administration of different synbiotics on carcass and meat quality traits in broiler chickens.					
30690616	0	35	from	Effect	0:5	arg1	carcass					59:65	carcass	59:65	carcass	59:65	Effect of in ovo administration of different synbiotics on carcass and meat quality traits in broiler chickens.					
30690616	12	36	from	birds	2042:2046	arg1	meat					2021:2024	meat	2021:2024	meat from C and SYN2 birds	2021:2046	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	2	37	theme	family	577:582	arg1	oligosaccharides					584:599	raffinose family oligosaccharides	567:599	raffinose family oligosaccharides	567:599	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	5	38	theme	investigated	845:856	arg1	traits					858:863	investigated traits	845:863	investigated traits	845:863	In ovo synbiotic administration had a low effect on investigated traits, but depends on the kind of synbiotic administered.					
30690616	10	39	theme	atherogenic	1792:1802	arg1	indices					1821:1827	lower (P < 0.01) atherogenic and thrombogenic indices	1775:1827	lower (P < 0.01) atherogenic and thrombogenic indices	1775:1827	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	8	40	theme	fatty	1532:1536	arg1	PUFA					1545:1548	PUFA	1545:1548	PUFA	1545:1548	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	40	theme	fatty	1532:1536	arg1	acids					1538:1542	polyunsaturated fatty acids	1516:1542	lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA)	1486:1549	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	41	theme	n-6	1552:1554	arg1	PUFA					1556:1559	n-6 PUFA	1552:1559	n-6 PUFA	1552:1559	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	6	42	theme	synbiotic	922:930	arg1	formulations					932:943	Both synbiotic formulations	917:943	Both synbiotic formulations	917:943	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	8	43	dep	acids	1442:1446	arg1	<					1451:1451	P < 0.05	1449:1456	P < 0.05 compared only to SYN2	1449:1478	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	0	44	theme	in	10:11	arg1	administration					17:30	in ovo administration	10:30	in ovo administration of different synbiotics	10:54	Effect of in ovo administration of different synbiotics on carcass and meat quality traits in broiler chickens.					
30690616	7	45	dep	markedly	1282:1289	arg1	<					1294:1294	P < 0.05	1292:1299	P < 0.05	1292:1299	Synbiotic administration reduced (P = 0.061) the lipid content compared with C group, markedly (P < 0.05) with synbiotic SYN2.					
30690616	8	46	theme	SYN1	1333:1336	arg1	birds					1338:1342	SYN1 birds	1333:1342	SYN1 birds	1333:1342	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	10	47	from	groups	1713:1718	arg1	Meat					1692:1695	Meat	1692:1695	Meat from C and SYN2 groups	1692:1718	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	0	48	theme	administration	17:30	arg1	Effect					0:5	Effect	0:5	Effect of in ovo administration of different synbiotics on carcass and meat quality	0:82	Effect of in ovo administration of different synbiotics on carcass and meat quality traits in broiler chickens.					
30690616	10	49	theme	PUFA	1759:1762	arg1	ratio					1750:1754	a higher (P < 0.01) ratio	1730:1754	a higher (P < 0.01) ratio of PUFA to SFA	1730:1769	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	0	50	theme	different	35:43	arg1	synbiotics					45:54	different synbiotics	35:54	different synbiotics	35:54	Effect of in ovo administration of different synbiotics on carcass and meat quality traits in broiler chickens.					
30690616	8	51	theme	higher	1356:1361	arg1	content					1374:1380	a higher (P < 0.01) content	1354:1380	a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA)	1354:1549	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	3	52	theme	240	619:621	arg1	males					623:627	240 males	619:627	240 males	619:627	After hatching, 240 males were randomly chosen (80 chicks per group) and split into 8 replicate pens (10 birds per pen).					
30690616	8	53	theme	lower	1486:1490	arg1	PUFA					1545:1548	PUFA	1545:1548	PUFA	1545:1548	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	53	theme	lower	1486:1490	arg1	acids					1538:1542	polyunsaturated fatty acids	1516:1542	lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA)	1486:1549	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	54	theme	acids	1401:1405	arg1	PUFA					1556:1559	n-6 PUFA	1552:1559	n-6 PUFA	1552:1559	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	54	theme	acids	1401:1405	arg1	PUFA					1569:1572	n-3 PUFA	1565:1572	n-3 PUFA	1565:1572	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	54	theme	acids	1401:1405	arg1	content					1374:1380	a higher (P < 0.01) content	1354:1380	a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA)	1354:1549	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	1	55	theme	administration	180:193	arg1	effect					153:158	the effect	149:158	the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens	149:262	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	7	56	theme	P	1292:1292	arg1	<					1294:1294	P < 0.05	1292:1299	P < 0.05	1292:1299	Synbiotic administration reduced (P = 0.061) the lipid content compared with C group, markedly (P < 0.05) with synbiotic SYN2.					
30690616	0	57	dep	in	10:11	arg1	ovo					13:15	ovo	13:15	ovo	13:15	Effect of in ovo administration of different synbiotics on carcass and meat quality traits in broiler chickens.					
30690616	8	58	theme	P	1364:1364	arg1	<					1366:1366	P < 0.01	1364:1371	P < 0.01	1364:1371	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	1	59	theme	slaughter	198:206	arg1	performance					208:218	slaughter performance and meat quality traits	198:242	performance	208:218	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	11	60	theme	in	1888:1889	arg1	administration					1895:1908	in ovo administration	1888:1908	in ovo administration of synbiotics	1888:1922	In conclusion, this study has shown that in ovo administration of synbiotics did not negatively affect slaughter performance and physicochemical properties of meat.					
30690616	6	61	theme	intramuscular	1099:1111	arg1	properties					1122:1131	intramuscular collagen properties	1099:1131	intramuscular collagen properties	1099:1131	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	2	62	theme	Cobb	302:305	arg1	eggs					296:299	5,850 eggs	290:299	5,850 eggs (Cobb 500 FF)	290:313	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	2	62	theme	Cobb	302:305	arg1	FF					311:312	Cobb 500 FF	302:312	Cobb 500 FF	302:312	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	12	63	theme	acid	2074:2077	arg1	profile					2079:2085	a preferable fatty acid profile	2055:2085	a preferable fatty acid profile	2055:2085	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	8	64	theme	monounsaturated	1420:1434	arg1	acids					1442:1446	lower monounsaturated fatty acids	1414:1446	lower monounsaturated fatty acids (P < 0.05 compared only to SYN2)	1414:1479	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	1	65	theme	quality	229:235	arg1	traits					237:242	slaughter performance and meat quality traits	198:242	traits	237:242	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	7	66	theme	P	1230:1230	arg1	0.061					1234:1238	P = 0.061	1230:1238	P = 0.061	1230:1238	Synbiotic administration reduced (P = 0.061) the lipid content compared with C group, markedly (P < 0.05) with synbiotic SYN2.					
30690616	10	67	theme	thrombogenic	1808:1819	arg1	indices					1821:1827	lower (P < 0.01) atherogenic and thrombogenic indices	1775:1827	lower (P < 0.01) atherogenic and thrombogenic indices	1775:1827	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	7	68	theme	lipid	1245:1249	arg1	content					1251:1257	the lipid content	1241:1257	the lipid content	1241:1257	Synbiotic administration reduced (P = 0.061) the lipid content compared with C group, markedly (P < 0.05) with synbiotic SYN2.					
30690616	12	69	theme	SYN2	2037:2040	arg1	birds					2042:2046	C and SYN2 birds	2031:2046	C and SYN2 birds	2031:2046	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	8	70	theme	saturated	1385:1393	arg1	SFA					1408:1410	SFA	1408:1410	SFA	1408:1410	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	70	theme	saturated	1385:1393	arg1	acids					1401:1405	saturated fatty acids	1385:1405	saturated fatty acids (SFA)	1385:1411	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	3	71	theme	per	661:663	arg1	group					665:669	80 chicks per group	651:669	80 chicks per group	651:669	After hatching, 240 males were randomly chosen (80 chicks per group) and split into 8 replicate pens (10 birds per pen).					
30690616	6	72	theme	holding	1080:1086	arg1	pH					1063:1064	pH	1063:1064	pH	1063:1064	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	72	theme	holding	1080:1086	arg1	capacity					1088:1095	water holding capacity	1074:1095	water holding capacity	1074:1095	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	8	73	theme	acids	1442:1446	arg1	PUFA					1556:1559	n-6 PUFA	1552:1559	n-6 PUFA	1552:1559	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	73	theme	acids	1442:1446	arg1	PUFA					1569:1572	n-3 PUFA	1565:1572	n-3 PUFA	1565:1572	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	73	theme	acids	1442:1446	arg1	content					1374:1380	a higher (P < 0.01) content	1354:1380	a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA)	1354:1549	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	6	74	theme	muscle	1012:1017	arg1	BW					966:967	final BW	960:967	final BW	960:967	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	74	theme	muscle	1012:1017	arg1	weight					970:975	weight	970:975	weight	970:975	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	74	theme	muscle	1012:1017	arg1	yield					982:986	yield	982:986	yield	982:986	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	8	75	theme	P	1449:1449	arg1	<					1451:1451	P < 0.05	1449:1456	P < 0.05 compared only to SYN2	1449:1478	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	7	76	theme	=	1232:1232	arg1	0.061					1234:1238	P = 0.061	1230:1238	P = 0.061	1230:1238	Synbiotic administration reduced (P = 0.061) the lipid content compared with C group, markedly (P < 0.05) with synbiotic SYN2.					
30690616	6	77	theme	PM	1161:1162	arg1	content					1150:1156	cholesterol content	1138:1156	cholesterol content of PM	1138:1162	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	77	theme	PM	1161:1162	arg1	properties					1122:1131	intramuscular collagen properties	1099:1131	intramuscular collagen properties	1099:1131	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	77	theme	PM	1161:1162	arg1	properties					1051:1060	physicochemical properties	1035:1060	physicochemical properties (pH, color, water holding capacity)	1035:1096	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	10	78	theme	lower	1775:1779	arg1	indices					1821:1827	lower (P < 0.01) atherogenic and thrombogenic indices	1775:1827	lower (P < 0.01) atherogenic and thrombogenic indices	1775:1827	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	11	79	theme	synbiotics	1913:1922	arg1	administration					1895:1908	in ovo administration	1888:1908	in ovo administration of synbiotics	1888:1922	In conclusion, this study has shown that in ovo administration of synbiotics did not negatively affect slaughter performance and physicochemical properties of meat.					
30690616	12	80	theme	C	2031:2031	arg1	birds					2042:2046	C and SYN2 birds	2031:2046	C and SYN2 birds	2031:2046	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	8	81	dep	higher	1356:1361	arg1	<					1366:1366	P < 0.01	1364:1371	P < 0.01	1364:1371	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	12	82	from	effect	2104:2109	arg1	properties					2126:2135	nutritional properties	2114:2135	nutritional properties of chicken meat	2114:2151	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	2	83	theme	synbiotic	453:461	arg1	formulations					463:474	2 different synbiotic formulations	441:474	2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides)	441:600	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	11	84	theme	physicochemical	1976:1990	arg1	properties					1992:2001	physicochemical properties	1976:2001	physicochemical properties	1976:2001	In conclusion, this study has shown that in ovo administration of synbiotics did not negatively affect slaughter performance and physicochemical properties of meat.					
30690616	9	85	theme	n-6	1618:1620	arg1	ratio					1609:1613	The ratio	1605:1613	The ratio of n-6 to n-3 PUFA	1605:1632	The ratio of n-6 to n-3 PUFA was affected by the synbiotic administration (P = 0.039).					
30690616	10	86	theme	P	1740:1740	arg1	higher					1732:1737	higher	1732:1737	higher	1732:1737	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	10	86	theme	P	1740:1740	arg1	<					1742:1742	P < 0.01	1740:1747	P < 0.01	1740:1747	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	2	87	with	ovo	394:396	arg1	control					425:431	control	425:431	control	425:431	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	2	87	with	ovo	394:396	arg1	formulations					463:474	2 different synbiotic formulations	441:474	2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides)	441:600	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	2	87	with	ovo	394:396	arg1	saline					417:422	physiological saline	403:422	physiological saline (control, C)	403:435	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	7	88	dep	reduced	1221:1227	arg1	0.061					1234:1238	P = 0.061	1230:1238	P = 0.061	1230:1238	Synbiotic administration reduced (P = 0.061) the lipid content compared with C group, markedly (P < 0.05) with synbiotic SYN2.					
30690616	9	89	theme	n-3	1625:1627	arg1	PUFA					1629:1632	n-3 PUFA	1625:1632	n-3 PUFA	1625:1632	The ratio of n-6 to n-3 PUFA was affected by the synbiotic administration (P = 0.039).					
30690616	6	90	dep	properties	1051:1060	arg1	color					1067:1071	color	1067:1071	color	1067:1071	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	90	dep	properties	1051:1060	arg1	pH					1063:1064	pH	1063:1064	pH	1063:1064	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	6	90	dep	properties	1051:1060	arg1	capacity					1088:1095	water holding capacity	1074:1095	water holding capacity	1074:1095	Both synbiotic formulations did not affect final BW, weight, and yield of carcass and pectoral muscle (PM); likewise, physicochemical properties (pH, color, water holding capacity), intramuscular collagen properties, and cholesterol content of PM were not affected by treatment.					
30690616	8	91	theme	P	1493:1493	arg1	<					1495:1495	P < 0.01 and P < 0.05	1493:1513	<	1495:1495	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	1	92	theme	study	128:132	arg1	aim					116:118	The aim	112:118	The aim of this study	112:132	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	10	93	dep	lower	1775:1779	arg1	P					1782:1782	P < 0.01	1782:1789	P < 0.01	1782:1789	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	10	94	theme	<	1784:1784	arg1	P					1782:1782	P < 0.01	1782:1789	P < 0.01	1782:1789	Meat from C and SYN2 groups displayed a higher (P < 0.01) ratio of PUFA to SFA and lower (P < 0.01) atherogenic and thrombogenic indices compared to SYN1.					
30690616	5	95	theme	low	831:833	arg1	effect					835:840	a low effect	829:840	a low effect	829:840	In ovo synbiotic administration had a low effect on investigated traits, but depends on the kind of synbiotic administered.					
30690616	12	96	theme	meat	2148:2151	arg1	properties					2126:2135	nutritional properties	2114:2135	nutritional properties of chicken meat	2114:2151	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	0	97	theme	broiler	94:100	arg1	chickens					102:109	broiler chickens	94:109	broiler chickens	94:109	Effect of in ovo administration of different synbiotics on carcass and meat quality traits in broiler chickens.					
30690616	8	98	theme	P	1506:1506	arg1	<					1508:1508	P < 0.01 and P < 0.05	1493:1513	<	1508:1508	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	9	99	theme	synbiotic	1654:1662	arg1	administration					1664:1677	the synbiotic administration	1650:1677	the synbiotic administration (P = 0.039)	1650:1689	The ratio of n-6 to n-3 PUFA was affected by the synbiotic administration (P = 0.039).					
30690616	9	99	theme	synbiotic	1654:1662	arg1	0.039					1684:1688	P = 0.039	1680:1688	P = 0.039	1680:1688	The ratio of n-6 to n-3 PUFA was affected by the synbiotic administration (P = 0.039).					
30690616	3	100	dep	pens	699:702	arg1	birds					708:712	10 birds	705:712	10 birds per pen	705:720	After hatching, 240 males were randomly chosen (80 chicks per group) and split into 8 replicate pens (10 birds per pen).					
30690616	3	100	dep	pens	699:702	arg1	replicate					689:697	replicate	689:697	replicate	689:697	After hatching, 240 males were randomly chosen (80 chicks per group) and split into 8 replicate pens (10 birds per pen).					
30690616	5	101	theme	synbiotic	800:808	arg1	administration					810:823	synbiotic administration	800:823	synbiotic administration	800:823	In ovo synbiotic administration had a low effect on investigated traits, but depends on the kind of synbiotic administered.					
30690616	12	102	theme	nutritional	2114:2124	arg1	properties					2126:2135	nutritional properties	2114:2135	nutritional properties of chicken meat	2114:2151	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	1	103	theme	broiler	247:253	arg1	chickens					255:262	broiler chickens	247:262	broiler chickens	247:262	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	8	104	theme	polyunsaturated	1516:1530	arg1	PUFA					1545:1548	PUFA	1545:1548	PUFA	1545:1548	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	104	theme	polyunsaturated	1516:1530	arg1	acids					1538:1542	polyunsaturated fatty acids	1516:1542	lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA)	1486:1549	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	0	105	dep	carcass	59:65	arg1	quality					76:82	quality	76:82	quality	76:82	Effect of in ovo administration of different synbiotics on carcass and meat quality traits in broiler chickens.					
30690616	12	106	theme	positive	2095:2102	arg1	effect					2104:2109	a positive effect	2093:2109	a positive effect on nutritional properties of chicken meat	2093:2151	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	9	107	theme	=	1682:1682	arg1	administration					1664:1677	the synbiotic administration	1650:1677	the synbiotic administration (P = 0.039)	1650:1689	The ratio of n-6 to n-3 PUFA was affected by the synbiotic administration (P = 0.039).					
30690616	9	107	theme	=	1682:1682	arg1	0.039					1684:1688	P = 0.039	1680:1688	P = 0.039	1680:1688	The ratio of n-6 to n-3 PUFA was affected by the synbiotic administration (P = 0.039).					
30690616	11	108	theme	meat	2006:2009	arg1	performance					1960:1970	slaughter performance	1950:1970	slaughter performance	1950:1970	In conclusion, this study has shown that in ovo administration of synbiotics did not negatively affect slaughter performance and physicochemical properties of meat.					
30690616	11	108	theme	meat	2006:2009	arg1	properties					1992:2001	physicochemical properties	1976:2001	physicochemical properties	1976:2001	In conclusion, this study has shown that in ovo administration of synbiotics did not negatively affect slaughter performance and physicochemical properties of meat.					
30690616	2	109	theme	experimental	344:355	arg1	groups					357:362	3 experimental groups	342:362	3 experimental groups	342:362	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	3	110	dep	chosen	643:648	arg1	group					665:669	80 chicks per group	651:669	80 chicks per group	651:669	After hatching, 240 males were randomly chosen (80 chicks per group) and split into 8 replicate pens (10 birds per pen).					
30690616	8	111	from	birds	1338:1342	arg1	Meat					1323:1326	Meat	1323:1326	Meat from SYN1 birds	1323:1342	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	2	112	theme	physiological	403:415	arg1	control					425:431	control	425:431	control	425:431	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	2	112	theme	physiological	403:415	arg1	saline					417:422	physiological saline	403:422	physiological saline (control, C)	403:435	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	0	113	theme	synbiotics	45:54	arg1	administration					17:30	in ovo administration	10:30	in ovo administration of different synbiotics	10:54	Effect of in ovo administration of different synbiotics on carcass and meat quality traits in broiler chickens.					
30690616	2	114	dep	control	425:431	arg1	C					434:434	C	434:434	C	434:434	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	7	115	theme	synbiotic	1307:1315	arg1	SYN2					1317:1320	synbiotic SYN2	1307:1320	synbiotic SYN2	1307:1320	Synbiotic administration reduced (P = 0.061) the lipid content compared with C group, markedly (P < 0.05) with synbiotic SYN2.					
30690616	2	116	theme	incubation	278:287	arg1	day					268:270	day 12	268:273	day 12 of incubation	268:287	On day 12 of incubation, 5,850 eggs (Cobb 500 FF) were randomly divided into 3 experimental groups and automatically injected in ovo with physiological saline (control, C) and 2 different synbiotic formulations (SYN1: Lactobacillus salivarius + galactooligosaccharides; SYN2: Lactobacillus plantarum + raffinose family oligosaccharides).					
30690616	12	117	theme	preferable	2057:2066	arg1	profile					2079:2085	a preferable fatty acid profile	2055:2085	a preferable fatty acid profile	2055:2085	However, meat from C and SYN2 birds showed a preferable fatty acid profile, with a positive effect on nutritional properties of chicken meat.					
30690616	8	118	dep	lower	1486:1490	arg1	<					1508:1508	P < 0.01 and P < 0.05	1493:1513	<	1508:1508	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	8	118	dep	lower	1486:1490	arg1	<					1495:1495	P < 0.01 and P < 0.05	1493:1513	<	1495:1495	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30690616	1	119	dep	in	163:164	arg1	ovo					166:168	ovo	166:168	ovo	166:168	The aim of this study was to examine the effect of in ovo synbiotic administration on slaughter performance and meat quality traits of broiler chickens.					
30690616	8	120	theme	lower	1414:1418	arg1	acids					1442:1446	lower monounsaturated fatty acids	1414:1446	lower monounsaturated fatty acids (P < 0.05 compared only to SYN2)	1414:1479	Meat from SYN1 birds displayed a higher (P < 0.01) content of saturated fatty acids (SFA), lower monounsaturated fatty acids (P < 0.05 compared only to SYN2), and lower (P < 0.01 and P < 0.05) polyunsaturated fatty acids (PUFA), n-6 PUFA and n-3 PUFA compared to C and SYN2 groups.					
30219335	2	0	theme	ions	457:460	arg1	interactions					376:387	metal ion coordination interactions	353:387	metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions	353:460	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	5	1	theme	batch	839:843	arg1	system					845:850	a batch system	837:850	a batch system	837:850	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system.					
30219335	9	2	with	depletion	1377:1385	arg1	reusability					1455:1465	reusability	1455:1465	reusability	1455:1465	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	9	2	with	depletion	1377:1385	arg1	capacity					1417:1424	a high adsorption capacity	1399:1424	a high adsorption capacity	1399:1424	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	9	2	with	depletion	1377:1385	arg1	selectivity					1439:1449	significant selectivity	1427:1449	significant selectivity	1427:1449	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	9	3	theme	template	1334:1341	arg1	binding					1323:1329	specific binding	1314:1329	specific binding of template	1314:1341	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	5	4	theme	adsorption	726:735	arg1	experiments					737:747	The adsorption experiments	722:747	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions	722:814	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system.					
30219335	8	5	theme	artificial	1149:1158	arg1	plasma					1166:1171	artificial human plasma	1149:1171	artificial human plasma	1149:1171	In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis.					
30219335	5	6	theme	HSA	752:754	arg1	experiments					737:747	The adsorption experiments	722:747	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions	722:814	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system.					
30219335	3	7	theme	cellulose	517:525	arg1	BCNFs					539:543	BCNFs	539:543	BCNFs	539:543	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	3	7	theme	cellulose	517:525	arg1	nanofibers					527:536	bacterial cellulose nanofibers	507:536	bacterial cellulose nanofibers (BCNFs)	507:544	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	1	8	theme	HSA	237:239	arg1	depletion					224:232	the depletion	220:232	the depletion of HSA selectively from artificial blood plasma for proteomic applications	220:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	9	9	theme	significant	1427:1437	arg1	selectivity					1439:1449	significant selectivity	1427:1449	significant selectivity	1427:1449	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	5	10	dep	MIP-BCNFs	765:773	arg1	the					761:763	the	761:763	the	761:763	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system.					
30219335	9	11	theme	HSA	1390:1392	arg1	depletion					1377:1385	the depletion	1373:1385	the depletion of HSA with a high adsorption capacity, significant selectivity and reusability	1373:1465	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	2	12	theme	Molecular	310:318	arg1	imprinting					320:329	Molecular imprinting	310:329	Molecular imprinting	310:329	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	9	13	theme	adsorption	1406:1415	arg1	capacity					1417:1424	a high adsorption capacity	1399:1424	a high adsorption capacity	1399:1424	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	5	14	theme	aqueous	794:800	arg1	solutions					806:814	aqueous HSA solutions	794:814	aqueous HSA solutions	794:814	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system.					
30219335	2	15	theme	N‑methacryloyl‑	392:406	arg1	monomer					438:444	N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer	392:444	N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer	392:444	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	7	16	theme	HSA/Myo	1077:1083	arg1	molecules					1085:1093	HSA/Myo molecules	1077:1093	HSA/Myo molecules	1077:1093	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	1	17	from	plasma	275:280	arg1	depletion					224:232	the depletion	220:232	the depletion of HSA selectively from artificial blood plasma for proteomic applications	220:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	1	18	theme	imprinted	147:155	arg1	nanofibers					187:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers	121:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications	121:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	1	18	theme	imprinted	147:155	arg1	MIP-cBCNFs					199:208	MIP-cBCNFs	199:208	MIP-cBCNFs	199:208	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	2	19	theme	monomer	438:444	arg1	interactions					376:387	metal ion coordination interactions	353:387	metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions	353:460	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	3	20	theme	-HSA	473:476	arg1	complex					478:484	MAH-Cu(II)-HSA complex	463:484	MAH-Cu(II)-HSA complex	463:484	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	3	21	from	temperature	578:588	arg1	polymerized					490:500	polymerized	490:500	polymerized	490:500	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	1	22	theme	composite	157:165	arg1	nanofibers					187:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers	121:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications	121:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	1	22	theme	composite	157:165	arg1	MIP-cBCNFs					199:208	MIP-cBCNFs	199:208	MIP-cBCNFs	199:208	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	0	23	theme	Protein	0:6	arg1	depletion					8:16	Protein depletion	0:16	Protein depletion with bacterial cellulose	0:41	Protein depletion with bacterial cellulose nanofibers.					
30219335	4	24	theme	contact	664:670	arg1	measurements					678:689	contact angle measurements	664:689	contact angle measurements	664:689	The characterization of the MIP-cBCNFs was carried out by FTIR-ATR, SEM, contact angle measurements and surface area measurements.					
30219335	2	25	theme	coordination	363:374	arg1	interactions					376:387	metal ion coordination interactions	353:387	metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions	353:460	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	0	26	theme	bacterial	23:31	arg1	cellulose					33:41	bacterial cellulose	23:41	bacterial cellulose	23:41	Protein depletion with bacterial cellulose nanofibers.					
30219335	1	27	theme	artificial	258:267	arg1	plasma					275:280	artificial blood plasma	258:280	artificial blood plasma for proteomic applications	258:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	6	28	theme	human	926:930	arg1	HTR					945:947	HTR	945:947	HTR	945:947	The selectivity of the MIP-cBCNFs was investigated by using non-template human transferrin (HTR), and myoglobin (Myo).					
30219335	6	28	theme	human	926:930	arg1	transferrin					932:942	non-template human transferrin	913:942	non-template human transferrin (HTR)	913:948	The selectivity of the MIP-cBCNFs was investigated by using non-template human transferrin (HTR), and myoglobin (Myo).					
30219335	3	29	theme	bacterial	507:515	arg1	BCNFs					539:543	BCNFs	539:543	BCNFs	539:543	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	3	29	theme	bacterial	507:515	arg1	nanofibers					527:536	bacterial cellulose nanofibers	507:536	bacterial cellulose nanofibers (BCNFs)	507:544	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	2	30	theme	‑histidinemethylester	410:430	arg1	monomer					438:444	N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer	392:444	N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer	392:444	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	1	31	theme	blood	269:273	arg1	plasma					275:280	artificial blood plasma	258:280	artificial blood plasma for proteomic applications	258:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	7	32	theme	MIP-cBCNFs	1017:1026	arg1	3.02					1056:1059	3.02	1056:1059	3.02	1056:1059	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	7	32	theme	MIP-cBCNFs	1017:1026	arg1	4.73					1047:1050	4.73	1047:1050	4.73	1047:1050	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	7	32	theme	MIP-cBCNFs	1017:1026	arg1	coefficients					997:1008	The relative selectivity coefficients	972:1008	The relative selectivity coefficients of the MIP-cBCNFs	972:1026	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	1	33	theme	novel	89:93	arg1	technique					107:115	a novel fabrication technique	87:115	a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications	87:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	1	34	theme	serum	127:131	arg1	albumin					133:139	human serum albumin	121:139	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications	121:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	1	34	theme	serum	127:131	arg1	HSA					142:144	HSA	142:144	HSA	142:144	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	6	35	theme	non-template	913:924	arg1	HTR					945:947	HTR	945:947	HTR	945:947	The selectivity of the MIP-cBCNFs was investigated by using non-template human transferrin (HTR), and myoglobin (Myo).					
30219335	6	35	theme	non-template	913:924	arg1	transferrin					932:942	non-template human transferrin	913:942	non-template human transferrin (HTR)	913:948	The selectivity of the MIP-cBCNFs was investigated by using non-template human transferrin (HTR), and myoglobin (Myo).					
30219335	5	36	theme	HSA	802:804	arg1	solutions					806:814	aqueous HSA solutions	794:814	aqueous HSA solutions	794:814	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system.					
30219335	1	37	theme	bacterial	167:175	arg1	nanofibers					187:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers	121:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications	121:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	1	37	theme	bacterial	167:175	arg1	MIP-cBCNFs					199:208	MIP-cBCNFs	199:208	MIP-cBCNFs	199:208	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	2	38	theme	l	408:408	arg1	monomer					438:444	N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer	392:444	N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer	392:444	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	4	39	theme	MIP-cBCNFs	619:628	arg1	characterization					595:610	The characterization	591:610	The characterization of the MIP-cBCNFs	591:628	The characterization of the MIP-cBCNFs was carried out by FTIR-ATR, SEM, contact angle measurements and surface area measurements.					
30219335	9	40	theme	ion	1270:1272	arg1	interactions					1287:1298	metal ion coordination interactions	1264:1298	metal ion coordination interactions	1264:1298	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	8	41	theme	HSA	1140:1142	arg1	depletion					1127:1135	the depletion	1123:1135	the depletion of HSA from artificial human plasma	1123:1171	In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis.					
30219335	8	42	theme	gel	1207:1209	arg1	electrophoresis					1211:1225	2-D gel electrophoresis	1203:1225	2-D gel electrophoresis	1203:1225	In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis.					
30219335	2	43	theme	MAH	433:435	arg1	monomer					438:444	N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer	392:444	N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer	392:444	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	8	44	theme	2-D	1203:1205	arg1	electrophoresis					1211:1225	2-D gel electrophoresis	1203:1225	2-D gel electrophoresis	1203:1225	In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis.					
30219335	2	45	theme	Cu	450:451	arg1	ions					457:460	Cu(II) ions	450:460	Cu(II) ions	450:460	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	1	46	theme	proteomic	286:294	arg1	applications					296:307	proteomic applications	286:307	proteomic applications	286:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	7	47	theme	relative	976:983	arg1	3.02					1056:1059	3.02	1056:1059	3.02	1056:1059	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	7	47	theme	relative	976:983	arg1	4.73					1047:1050	4.73	1047:1050	4.73	1047:1050	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	7	47	theme	relative	976:983	arg1	coefficients					997:1008	The relative selectivity coefficients	972:1008	The relative selectivity coefficients of the MIP-cBCNFs	972:1026	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	5	48	from	solutions	806:814	arg1	MIP-BCNFs					765:773	MIP-BCNFs	765:773	MIP-BCNFs	765:773	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system.					
30219335	5	48	from	solutions	806:814	arg1	NIP-BCNFs					779:787	NIP-BCNFs	779:787	NIP-BCNFs	779:787	The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system.					
30219335	1	49	theme	fabrication	95:105	arg1	technique					107:115	a novel fabrication technique	87:115	a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications	87:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	2	50	theme	metal	353:357	arg1	interactions					376:387	metal ion coordination interactions	353:387	metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions	353:460	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	7	51	theme	selectivity	985:995	arg1	3.02					1056:1059	3.02	1056:1059	3.02	1056:1059	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	7	51	theme	selectivity	985:995	arg1	4.73					1047:1050	4.73	1047:1050	4.73	1047:1050	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	7	51	theme	selectivity	985:995	arg1	coefficients					997:1008	The relative selectivity coefficients	972:1008	The relative selectivity coefficients of the MIP-cBCNFs	972:1026	The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively.					
30219335	0	52	with	depletion	8:16	arg1	cellulose					33:41	bacterial cellulose	23:41	bacterial cellulose	23:41	Protein depletion with bacterial cellulose nanofibers.					
30219335	3	53	theme	MAH-Cu	463:468	arg1	complex					478:484	MAH-Cu(II)-HSA complex	463:484	MAH-Cu(II)-HSA complex	463:484	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	3	54	theme	room	573:576	arg1	temperature					578:588	room temperature	573:588	room temperature	573:588	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	3	55	theme	constant	552:559	arg1	stirring					561:568	constant stirring	552:568	constant stirring	552:568	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	4	56	theme	angle	672:676	arg1	measurements					678:689	contact angle measurements	664:689	contact angle measurements	664:689	The characterization of the MIP-cBCNFs was carried out by FTIR-ATR, SEM, contact angle measurements and surface area measurements.					
30219335	4	57	theme	area	703:706	arg1	measurements					708:719	surface area measurements	695:719	surface area measurements	695:719	The characterization of the MIP-cBCNFs was carried out by FTIR-ATR, SEM, contact angle measurements and surface area measurements.					
30219335	8	58	from	plasma	1166:1171	arg1	depletion					1127:1135	the depletion	1123:1135	the depletion of HSA from artificial human plasma	1123:1171	In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis.					
30219335	4	59	theme	surface	695:701	arg1	measurements					708:719	surface area measurements	695:719	surface area measurements	695:719	The characterization of the MIP-cBCNFs was carried out by FTIR-ATR, SEM, contact angle measurements and surface area measurements.					
30219335	1	60	theme	human	121:125	arg1	albumin					133:139	human serum albumin	121:139	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications	121:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	1	60	theme	human	121:125	arg1	HSA					142:144	HSA	142:144	HSA	142:144	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	8	61	theme	human	1160:1164	arg1	plasma					1166:1171	artificial human plasma	1149:1171	artificial human plasma	1149:1171	In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis.					
30219335	9	62	theme	specific	1314:1321	arg1	binding					1323:1329	specific binding	1314:1329	specific binding of template	1314:1341	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	9	63	theme	high	1401:1404	arg1	capacity					1417:1424	a high adsorption capacity	1399:1424	a high adsorption capacity	1399:1424	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	1	64	theme	cellulose	177:185	arg1	nanofibers					187:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers	121:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications	121:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	1	64	theme	cellulose	177:185	arg1	MIP-cBCNFs					199:208	MIP-cBCNFs	199:208	MIP-cBCNFs	199:208	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	2	65	theme	ion	359:361	arg1	interactions					376:387	metal ion coordination interactions	353:387	metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions	353:460	Molecular imprinting was achieved by using metal ion coordination interactions of N‑methacryloyl‑(l)‑histidinemethylester (MAH) monomer and Cu(II) ions.					
30219335	9	66	theme	metal	1264:1268	arg1	interactions					1287:1298	metal ion coordination interactions	1264:1298	metal ion coordination interactions	1264:1298	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
30219335	3	67	with	polymerized	490:500	arg1	BCNFs					539:543	BCNFs	539:543	BCNFs	539:543	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	3	67	with	polymerized	490:500	arg1	nanofibers					527:536	bacterial cellulose nanofibers	507:536	bacterial cellulose nanofibers (BCNFs)	507:544	MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature.					
30219335	6	68	theme	MIP-cBCNFs	876:885	arg1	selectivity					857:867	The selectivity	853:867	The selectivity of the MIP-cBCNFs	853:885	The selectivity of the MIP-cBCNFs was investigated by using non-template human transferrin (HTR), and myoglobin (Myo).					
30219335	1	69	theme	albumin	133:139	arg1	nanofibers					187:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers	121:196	human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications	121:307	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	1	69	theme	albumin	133:139	arg1	MIP-cBCNFs					199:208	MIP-cBCNFs	199:208	MIP-cBCNFs	199:208	In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications.					
30219335	9	70	theme	coordination	1274:1285	arg1	interactions					1287:1298	metal ion coordination interactions	1264:1298	metal ion coordination interactions	1264:1298	As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability.					
29728339	5	0	theme	central	808:814	arg1	design					837:842	a central composite rotational design	806:842	a central composite rotational design	806:842	To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used.					
29728339	7	1	theme	biosurfactant	1021:1033	arg1	molecule					1035:1042	the biosurfactant molecule	1017:1042	the biosurfactant molecule	1017:1042	The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry.					
29728339	3	2	theme	low	521:523	arg1	cost					525:528	a low cost	519:528	a low cost culture medium	519:543	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	5	3	theme	composite	816:824	arg1	design					837:842	a central composite rotational design	806:842	a central composite rotational design	806:842	To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used.					
29728339	3	4	theme	cost	525:528	arg1	medium					538:543	a low cost culture medium	519:543	a low cost culture medium	519:543	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	6	5	theme	inoculum	944:951	arg1	pH					884:885	pH	884:885	pH	884:885	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	6	5	theme	inoculum	944:951	arg1	agitation					920:928	agitation	920:928	agitation	920:928	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	6	5	theme	inoculum	944:951	arg1	amount					934:939	amount	934:939	amount	934:939	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	6	5	theme	inoculum	944:951	arg1	variables					859:867	The variables	855:867	The variables evaluated	855:877	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	6	5	theme	inoculum	944:951	arg1	period					899:904	incubation period	888:904	incubation period	888:904	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	6	5	theme	inoculum	944:951	arg1	temperature					907:917	temperature	907:917	temperature	907:917	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	6	5	theme	inoculum	944:951	arg1	inoculum					944:951	inoculum	944:951	inoculum	944:951	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	10	6	theme	trehalolipids	1525:1537	arg1	class					1516:1520	the class	1512:1520	the class of trehalolipids	1512:1537	The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.					
29728339	3	7	theme	present	388:394	arg1	work					396:399	The present work	384:399	The present work	384:399	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	9	8	theme	α	1351:1351	arg1	presence					1336:1343	the presence	1332:1343	the presence of an α,β-trehalose	1332:1363	The partial identification of the structure of the biosurfactant demonstrated the presence of an α,β-trehalose.					
29728339	10	9	theme	biosynthesis	1411:1422	arg1	study					1378:1382	The present study	1366:1382	The present study	1366:1382	The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.					
29728339	10	9	theme	biosynthesis	1411:1422	arg1	report					1397:1402	the first report	1387:1402	the first report of the biosynthesis of this compound by F. fujikuroi	1387:1455	The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.					
29728339	5	10	theme	Plackett-Burman	779:793	arg1	design					795:800	a Plackett-Burman design	777:800	a Plackett-Burman design	777:800	To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used.					
29728339	8	11	theme	days	1212:1215	arg1	conditions					1179:1188	the optimized conditions	1165:1188	the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation	1165:1251	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	0	12	with	biosurfactant	43:55	arg1	α					62:62	α	62:62	α	62:62	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	0	12	with	biosurfactant	43:55	arg1	β-trehalose					64:74	β-trehalose	64:74	β-trehalose	64:74	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	3	13	theme	biosurfactant	450:462	arg1	capacity					475:482	high biosurfactant production capacity	445:482	high biosurfactant production capacity	445:482	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	0	14	theme	Fusarium	79:86	arg1	fujikuroi					88:96	Fusarium fujikuroi	79:96	Fusarium fujikuroi under optimized conditions of submerged fermentation	79:149	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	8	15	from	20mNm-1	1151:1157	arg1	tension					1132:1138	surface tension	1124:1138	surface tension from 72 to 20mNm-1	1124:1157	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	6	16	from	pH	884:885	arg1	medium					965:970	a liquid medium	956:970	a liquid medium containing glucose	956:989	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	2	17	theme	production	286:295	arg1	capacities					297:306	high production capacities	281:306	high production capacities for these molecules	281:326	Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost.					
29728339	1	18	theme	many	172:175	arg1	advantages					177:186	many advantages	172:186	many advantages over synthetic surfactants	172:213	Biosurfactants have many advantages over synthetic surfactants but have higher production costs.					
29728339	8	19	theme	incubation	1220:1229	arg1	pH					1193:1194	pH 5.0	1193:1198	pH 5.0	1193:1198	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	8	19	theme	incubation	1220:1229	arg1	37°C					1201:1204	37°C	1201:1204	37°C	1201:1204	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	8	19	theme	incubation	1220:1229	arg1	days					1212:1215	7 days	1210:1215	7 days of incubation with 190rpm agitation	1210:1251	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	8	20	dep	20mNm-1	1151:1157	arg1	to					1148:1149	to	1148:1149	to	1148:1149	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	0	21	theme	First	0:4	arg1	report					6:11	First report	0:11	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.	0:150	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	10	22	theme	compound	1432:1439	arg1	biosynthesis					1411:1422	the biosynthesis	1407:1422	the biosynthesis of this compound by F. fujikuroi	1407:1455	The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.					
29728339	8	23	theme	surface	1124:1130	arg1	tension					1132:1138	surface tension	1124:1138	surface tension from 72 to 20mNm-1	1124:1157	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	4	24	attach	isolated	651:658	arg1	soil					665:668	soil	665:668	soil contaminated with hydrocarbons	665:699	The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi.					
29728339	4	24	attach	isolated	651:658	arg2	UFSM-BAS-01					635:645	The fungal strain UFSM-BAS-01	617:645	The fungal strain UFSM-BAS-01	617:645	The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi.					
29728339	4	24	attach	isolated	651:658	arg2	fujikuroi					728:736	Fusarium fujikuroi	719:736	Fusarium fujikuroi	719:736	The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi.					
29728339	3	25	theme	high	445:448	arg1	capacity					475:482	high biosurfactant production capacity	445:482	high biosurfactant production capacity	445:482	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	8	26	theme	190rpm	1236:1241	arg1	agitation					1243:1251	190rpm agitation	1236:1251	190rpm agitation	1236:1251	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	0	27	theme	optimized	104:112	arg1	conditions					114:123	optimized conditions	104:123	optimized conditions of submerged fermentation	104:149	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	7	28	theme	partial	996:1002	arg1	structure					1004:1012	The partial structure	992:1012	The partial structure of the biosurfactant molecule	992:1042	The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry.					
29728339	3	29	theme	chemical	567:574	arg1	structure					576:584	the chemical structure	563:584	the chemical structure of the biosurfactant molecule	563:614	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	1	30	theme	synthetic	193:201	arg1	surfactants					203:213	synthetic surfactants	193:213	synthetic surfactants	193:213	Biosurfactants have many advantages over synthetic surfactants but have higher production costs.					
29728339	6	31	from	temperature	907:917	arg1	medium					965:970	a liquid medium	956:970	a liquid medium containing glucose	956:989	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	6	32	theme	incubation	888:897	arg1	period					899:904	incubation period	888:904	incubation period	888:904	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	10	33	theme	present	1370:1376	arg1	study					1378:1382	The present study	1366:1382	The present study	1366:1382	The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.					
29728339	10	33	theme	present	1370:1376	arg1	report					1397:1402	the first report	1387:1402	the first report of the biosynthesis of this compound by F. fujikuroi	1387:1455	The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.					
29728339	9	34	theme	structure	1288:1296	arg1	identification					1266:1279	The partial identification	1254:1279	The partial identification of the structure of the biosurfactant	1254:1317	The partial identification of the structure of the biosurfactant demonstrated the presence of an α,β-trehalose.					
29728339	4	35	theme	Fusarium	719:726	arg1	fujikuroi					728:736	Fusarium fujikuroi	719:736	Fusarium fujikuroi	719:736	The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi.					
29728339	4	35	theme	Fusarium	719:726	arg1	UFSM-BAS-01					635:645	The fungal strain UFSM-BAS-01	617:645	The fungal strain UFSM-BAS-01	617:645	The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi.					
29728339	5	36	theme	rotational	826:835	arg1	design					837:842	a central composite rotational design	806:842	a central composite rotational design	806:842	To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used.					
29728339	5	37	theme	biosurfactant	751:763	arg1	production					765:774	biosurfactant production	751:774	biosurfactant production	751:774	To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used.					
29728339	0	38	theme	production	20:29	arg1	report					6:11	First report	0:11	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.	0:150	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	2	39	with	microorganisms	261:274	arg1	capacities					297:306	high production capacities	281:306	high production capacities for these molecules	281:326	Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost.					
29728339	5	40	used	used	849:852	arg2	design					795:800	a Plackett-Burman design	777:800	a Plackett-Burman design	777:800	To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used.					
29728339	5	40	used	used	849:852	arg2	design					837:842	a central composite rotational design	806:842	a central composite rotational design	806:842	To optimize biosurfactant production, a Plackett-Burman design and a central composite rotational design were used.					
29728339	7	41	theme	nuclear	1062:1068	arg1	resonance					1079:1087	nuclear magnetic resonance	1062:1087	nuclear magnetic resonance spectrometry	1062:1100	The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry.					
29728339	6	42	from	amount	934:939	arg1	medium					965:970	a liquid medium	956:970	a liquid medium containing glucose	956:989	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	0	43	theme	potent	36:41	arg1	biosurfactant					43:55	a potent biosurfactant	34:55	a potent biosurfactant with α,β-trehalose	34:74	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	0	44	theme	fermentation	138:149	arg1	conditions					114:123	optimized conditions	104:123	optimized conditions of submerged fermentation	104:149	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	1	45	contain	have	167:170	arg2	advantages					177:186	many advantages	172:186	many advantages over synthetic surfactants	172:213	Biosurfactants have many advantages over synthetic surfactants but have higher production costs.					
29728339	1	45	contain	have	167:170	arg1	Biosurfactants					152:165	Biosurfactants	152:165	Biosurfactants	152:165	Biosurfactants have many advantages over synthetic surfactants but have higher production costs.					
29728339	3	46	theme	biosurfactant	593:605	arg1	molecule					607:614	the biosurfactant molecule	589:614	the biosurfactant molecule	589:614	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	7	47	theme	magnetic	1070:1077	arg1	resonance					1079:1087	nuclear magnetic resonance	1062:1087	nuclear magnetic resonance spectrometry	1062:1100	The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry.					
29728339	4	48	theme	fungal	621:626	arg1	fujikuroi					728:736	Fusarium fujikuroi	719:736	Fusarium fujikuroi	719:736	The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi.					
29728339	4	48	theme	fungal	621:626	arg1	UFSM-BAS-01					635:645	The fungal strain UFSM-BAS-01	617:645	The fungal strain UFSM-BAS-01	617:645	The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi.					
29728339	0	49	theme	submerged	128:136	arg1	fermentation					138:149	submerged fermentation	128:149	submerged fermentation	128:149	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	1	50	contain	have	219:222	arg2	costs					242:246	higher production costs	224:246	higher production costs	224:246	Biosurfactants have many advantages over synthetic surfactants but have higher production costs.					
29728339	1	50	contain	have	219:222	arg1	Biosurfactants					152:165	Biosurfactants	152:165	Biosurfactants	152:165	Biosurfactants have many advantages over synthetic surfactants but have higher production costs.					
29728339	3	51	theme	molecule	607:614	arg1	structure					576:584	the chemical structure	563:584	the chemical structure of the biosurfactant molecule	563:614	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	8	52	with	incubation	1220:1229	arg1	agitation					1243:1251	190rpm agitation	1236:1251	190rpm agitation	1236:1251	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	9	53	theme	partial	1258:1264	arg1	identification					1266:1279	The partial identification	1254:1279	The partial identification of the structure of the biosurfactant	1254:1317	The partial identification of the structure of the biosurfactant demonstrated the presence of an α,β-trehalose.					
29728339	7	54	theme	resonance	1079:1087	arg1	spectrometry					1089:1100	nuclear magnetic resonance spectrometry	1062:1100	nuclear magnetic resonance spectrometry	1062:1100	The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry.					
29728339	8	55	theme	37°C	1201:1204	arg1	conditions					1179:1188	the optimized conditions	1165:1188	the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation	1165:1251	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	2	56	theme	high	281:284	arg1	capacities					297:306	high production capacities	281:306	high production capacities for these molecules	281:326	Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost.					
29728339	6	57	from	period	899:904	arg1	medium					965:970	a liquid medium	956:970	a liquid medium containing glucose	956:989	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	3	58	theme	growth	498:503	arg1	conditions					505:514	its growth conditions	494:514	its growth conditions	494:514	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	1	59	theme	higher	224:229	arg1	costs					242:246	higher production costs	224:246	higher production costs	224:246	Biosurfactants have many advantages over synthetic surfactants but have higher production costs.					
29728339	3	60	with	fungus	433:438	arg1	capacity					475:482	high biosurfactant production capacity	445:482	high biosurfactant production capacity	445:482	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	9	61	theme	biosurfactant	1305:1317	arg1	structure					1288:1296	the structure	1284:1296	the structure of the biosurfactant	1284:1317	The partial identification of the structure of the biosurfactant demonstrated the presence of an α,β-trehalose.					
29728339	6	62	contain	containing	972:981	arg1	medium					965:970	a liquid medium	956:970	a liquid medium containing glucose	956:989	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	6	62	contain	containing	972:981	arg2	glucose					983:989	glucose	983:989	glucose	983:989	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	7	63	theme	molecule	1035:1042	arg1	structure					1004:1012	The partial structure	992:1012	The partial structure of the biosurfactant molecule	992:1042	The partial structure of the biosurfactant molecule was identified by nuclear magnetic resonance spectrometry.					
29728339	2	64	theme	growth	349:354	arg1	conditions					356:365	their growth conditions	343:365	their growth conditions	343:365	Identifying microorganisms with high production capacities for these molecules and optimizing their growth conditions can reduce cost.					
29728339	1	65	theme	production	231:240	arg1	costs					242:246	higher production costs	224:246	higher production costs	224:246	Biosurfactants have many advantages over synthetic surfactants but have higher production costs.					
29728339	8	66	theme	pH	1193:1194	arg1	conditions					1179:1188	the optimized conditions	1165:1188	the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation	1165:1251	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	3	67	theme	culture	530:536	arg1	medium					538:543	a low cost culture medium	519:543	a low cost culture medium	519:543	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	3	68	theme	production	464:473	arg1	capacity					475:482	high biosurfactant production capacity	445:482	high biosurfactant production capacity	445:482	The present work aimed to isolate and identify a fungus with high biosurfactant production capacity, optimize its growth conditions in a low cost culture medium, and characterize the chemical structure of the biosurfactant molecule.					
29728339	0	69	theme	biosurfactant	43:55	arg1	production					20:29	the production	16:29	the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation	16:149	First report of the production of a potent biosurfactant with α,β-trehalose by Fusarium fujikuroi under optimized conditions of submerged fermentation.					
29728339	6	70	theme	liquid	958:963	arg1	medium					965:970	a liquid medium	956:970	a liquid medium containing glucose	956:989	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
29728339	10	71	theme	first	1391:1395	arg1	study					1378:1382	The present study	1366:1382	The present study	1366:1382	The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.					
29728339	10	71	theme	first	1391:1395	arg1	report					1397:1402	the first report	1387:1402	the first report of the biosynthesis of this compound by F. fujikuroi	1387:1455	The present study is the first report of the biosynthesis of this compound by F. fujikuroi, suggesting that the biosurfactant produced belongs to the class of trehalolipids.					
29728339	4	72	theme	strain	628:633	arg1	fujikuroi					728:736	Fusarium fujikuroi	719:736	Fusarium fujikuroi	719:736	The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi.					
29728339	4	72	theme	strain	628:633	arg1	UFSM-BAS-01					635:645	The fungal strain UFSM-BAS-01	617:645	The fungal strain UFSM-BAS-01	617:645	The fungal strain UFSM-BAS-01 was isolated from soil contaminated with hydrocarbons and identified as Fusarium fujikuroi.					
29728339	8	73	theme	optimized	1169:1177	arg1	conditions					1179:1188	the optimized conditions	1165:1188	the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation	1165:1251	F. fujikuroi reduced surface tension from 72 to 20mNm-1 under the optimized conditions of pH 5.0, 37°C and 7 days of incubation with 190rpm agitation.					
29728339	6	74	from	agitation	920:928	arg1	medium					965:970	a liquid medium	956:970	a liquid medium containing glucose	956:989	The variables evaluated were pH, incubation period, temperature, agitation and amount of inoculum in a liquid medium containing glucose.					
31536448	10	0	theme	microecological	1475:1489	arg1	closer					1550:1555	closer	1550:1555	closer	1550:1555	The intestinal microecological portion of the treatment group partially recovered and was closer to the normal group.					
31536448	10	0	theme	microecological	1475:1489	arg1	portion					1491:1497	The intestinal microecological portion	1460:1497	The intestinal microecological portion of the treatment group	1460:1520	The intestinal microecological portion of the treatment group partially recovered and was closer to the normal group.					
31536448	12	1	theme	nutritional	1848:1858	arg1	supplement					1860:1869	an active nutritional supplement	1838:1869	an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis	1838:1979	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	12	1	theme	nutritional	1848:1858	arg1	β-glucans					1811:1819	β-glucans	1811:1819	β-glucans	1811:1819	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	7	2	theme	intestinal	869:878	arg1	function					888:895	intestinal transit function	869:895	intestinal transit function of mice	869:903	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	7	2	theme	intestinal	869:878	arg1	frequency					858:866	the defecation frequency	843:866	the defecation frequency	843:866	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	0	3	theme	Loperamide-Induced	59:76	arg1	Constipation					78:89	Loperamide-Induced Constipation	59:89	Loperamide-Induced Constipation	59:89	Effects of Bread Yeast Cell Wall Beta-Glucans on Mice with Loperamide-Induced Constipation.					
31536448	4	4	theme	pharmacological	520:534	arg1	effects					536:542	various pharmacological effects	512:542	various pharmacological effects	512:542	β-glucans are natural polysaccharides widely found in yeast, fungus, and plants, which have been reported to exhibit various pharmacological effects.					
31536448	10	5	theme	intestinal	1464:1473	arg1	closer					1550:1555	closer	1550:1555	closer	1550:1555	The intestinal microecological portion of the treatment group partially recovered and was closer to the normal group.					
31536448	10	5	theme	intestinal	1464:1473	arg1	portion					1491:1497	The intestinal microecological portion	1460:1497	The intestinal microecological portion of the treatment group	1460:1520	The intestinal microecological portion of the treatment group partially recovered and was closer to the normal group.					
31536448	1	6	theme	intestinal	171:180	arg1	habits					182:187	intestinal habits	171:187	intestinal habits	171:187	Constipation is a common gastrointestinal disorder characterized by changes in intestinal habits.					
31536448	7	7	theme	defecation	847:856	arg1	function					888:895	intestinal transit function	869:895	intestinal transit function of mice	869:903	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	7	7	theme	defecation	847:856	arg1	frequency					858:866	the defecation frequency	843:866	the defecation frequency	843:866	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	9	8	theme	TJP	1447:1449	arg1	expression					1411:1420	the expression	1407:1420	the expression of neurotransmitters and TJP in mice	1407:1457	β-glucans significantly enhanced intestinal motility and provided a possibility to regulate the expression of neurotransmitters and TJP in mice.					
31536448	12	9	dep	constipation	1940:1951	arg1	complications					1953:1965	complications	1953:1965	complications	1953:1965	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	0	10	from	Effects	0:6	arg1	Mice					49:52	Mice	49:52	Mice with Loperamide-Induced Constipation	49:89	Effects of Bread Yeast Cell Wall Beta-Glucans on Mice with Loperamide-Induced Constipation.					
31536448	5	11	theme	loperamide-induced	655:672	arg1	mice					687:690	loperamide-induced constipation mice	655:690	loperamide-induced constipation mice	655:690	The aim of this study was to characterize the effect of β-glucans extracted from the bread yeast cell wall on loperamide-induced constipation mice.					
31536448	4	12	theme	natural	409:415	arg1	β-glucans					395:403	β-glucans	395:403	β-glucans	395:403	β-glucans are natural polysaccharides widely found in yeast, fungus, and plants, which have been reported to exhibit various pharmacological effects.					
31536448	4	12	theme	natural	409:415	arg1	polysaccharides					417:431	natural polysaccharides	409:431	natural polysaccharides	409:431	β-glucans are natural polysaccharides widely found in yeast, fungus, and plants, which have been reported to exhibit various pharmacological effects.					
31536448	3	13	theme	non-prescriptional	365:382	arg1	laxatives					384:392	non-prescriptional laxatives	365:392	non-prescriptional laxatives	365:392	Meanwhile, more than 50% of patients are dissatisfied with sense of use of non-prescriptional laxatives.					
31536448	11	14	theme	neurotransmitters	1714:1730	arg1	express					1703:1709	the express	1699:1709	the express of neurotransmitters and TJP to recover intestinal epithelial mechanical barrier	1699:1790	This study showed that β-glucans can influence the intestinal microbiota and restore microecological balance to regulate the express of neurotransmitters and TJP to recover intestinal epithelial mechanical barrier.					
31536448	5	15	theme	constipation	674:685	arg1	mice					687:690	loperamide-induced constipation mice	655:690	loperamide-induced constipation mice	655:690	The aim of this study was to characterize the effect of β-glucans extracted from the bread yeast cell wall on loperamide-induced constipation mice.					
31536448	12	16	used	used	1830:1833	arg2	supplement					1860:1869	an active nutritional supplement	1838:1869	an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis	1838:1979	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	12	16	used	used	1830:1833	arg2	β-glucans					1811:1819	β-glucans	1811:1819	β-glucans	1811:1819	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	8	17	theme	possible	1284:1291	arg1	mechanisms					1303:1312	the possible molecular mechanisms	1280:1312	the possible molecular mechanisms	1280:1312	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	10	18	theme	treatment	1506:1514	arg1	group					1516:1520	the treatment group	1502:1520	the treatment group	1502:1520	The intestinal microecological portion of the treatment group partially recovered and was closer to the normal group.					
31536448	0	19	with	Mice	49:52	arg1	Constipation					78:89	Loperamide-Induced Constipation	59:89	Loperamide-Induced Constipation	59:89	Effects of Bread Yeast Cell Wall Beta-Glucans on Mice with Loperamide-Induced Constipation.					
31536448	7	20	used	used	917:920	arg2	We					831:832	We	831:832	We	831:832	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	3	21	theme	use	358:360	arg1	sense					349:353	sense	349:353	sense of use of non-prescriptional laxatives	349:392	Meanwhile, more than 50% of patients are dissatisfied with sense of use of non-prescriptional laxatives.					
31536448	5	22	theme	bread	630:634	arg1	wall					647:650	the bread yeast cell wall	626:650	the bread yeast cell wall	626:650	The aim of this study was to characterize the effect of β-glucans extracted from the bread yeast cell wall on loperamide-induced constipation mice.					
31536448	8	23	theme	tight	1178:1182	arg1	protein					1193:1199	tight junction protein	1178:1199	tight junction protein (TJP) including zonula occludens-1 and mucin-2	1178:1246	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	8	23	theme	tight	1178:1182	arg1	TJP					1202:1204	TJP	1202:1204	TJP	1202:1204	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	8	24	theme	junction	1184:1191	arg1	protein					1193:1199	tight junction protein	1178:1199	tight junction protein (TJP) including zonula occludens-1 and mucin-2	1178:1246	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	8	24	theme	junction	1184:1191	arg1	TJP					1202:1204	TJP	1202:1204	TJP	1202:1204	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	10	25	theme	normal	1564:1569	arg1	group					1571:1575	the normal group	1560:1575	the normal group	1560:1575	The intestinal microecological portion of the treatment group partially recovered and was closer to the normal group.					
31536448	5	26	theme	yeast	636:640	arg1	wall					647:650	the bread yeast cell wall	626:650	the bread yeast cell wall	626:650	The aim of this study was to characterize the effect of β-glucans extracted from the bread yeast cell wall on loperamide-induced constipation mice.					
31536448	12	27	theme	intestinal	1894:1903	arg1	barrier					1905:1911	the damaged intestinal barrier	1882:1911	the damaged intestinal barrier	1882:1911	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	4	28	located	found	440:444	arg2	polysaccharides					417:431	natural polysaccharides	409:431	natural polysaccharides	409:431	β-glucans are natural polysaccharides widely found in yeast, fungus, and plants, which have been reported to exhibit various pharmacological effects.					
31536448	4	28	located	found	440:444	arg1	fungus					456:461	fungus	456:461	fungus	456:461	β-glucans are natural polysaccharides widely found in yeast, fungus, and plants, which have been reported to exhibit various pharmacological effects.					
31536448	4	28	located	found	440:444	arg1	plants					468:473	plants	468:473	plants	468:473	β-glucans are natural polysaccharides widely found in yeast, fungus, and plants, which have been reported to exhibit various pharmacological effects.					
31536448	4	28	located	found	440:444	arg1	yeast					449:453	yeast	449:453	yeast	449:453	β-glucans are natural polysaccharides widely found in yeast, fungus, and plants, which have been reported to exhibit various pharmacological effects.					
31536448	4	28	located	found	440:444	arg2	β-glucans					395:403	β-glucans	395:403	β-glucans	395:403	β-glucans are natural polysaccharides widely found in yeast, fungus, and plants, which have been reported to exhibit various pharmacological effects.					
31536448	9	29	theme	intestinal	1348:1357	arg1	motility					1359:1366	intestinal motility	1348:1366	intestinal motility	1348:1366	β-glucans significantly enhanced intestinal motility and provided a possibility to regulate the expression of neurotransmitters and TJP in mice.					
31536448	0	30	theme	Yeast	17:21	arg1	Beta-Glucans					33:44	Bread Yeast Cell Wall Beta-Glucans	11:44	Bread Yeast Cell Wall Beta-Glucans	11:44	Effects of Bread Yeast Cell Wall Beta-Glucans on Mice with Loperamide-Induced Constipation.					
31536448	11	31	theme	intestinal	1751:1760	arg1	barrier					1784:1790	intestinal epithelial mechanical barrier	1751:1790	intestinal epithelial mechanical barrier	1751:1790	This study showed that β-glucans can influence the intestinal microbiota and restore microecological balance to regulate the express of neurotransmitters and TJP to recover intestinal epithelial mechanical barrier.					
31536448	3	32	theme	laxatives	384:392	arg1	use					358:360	use	358:360	use of non-prescriptional laxatives	358:392	Meanwhile, more than 50% of patients are dissatisfied with sense of use of non-prescriptional laxatives.					
31536448	8	33	theme	neurotransmitters	1076:1092	arg1	expression					1164:1173	expression	1164:1173	expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2	1164:1246	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	8	33	theme	neurotransmitters	1076:1092	arg1	expression					1062:1071	expression	1062:1071	expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT)	1062:1158	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	1	34	from	changes	160:166	arg1	habits					182:187	intestinal habits	171:187	intestinal habits	171:187	Constipation is a common gastrointestinal disorder characterized by changes in intestinal habits.					
31536448	12	35	theme	damaged	1886:1892	arg1	barrier					1905:1911	the damaged intestinal barrier	1882:1911	the damaged intestinal barrier	1882:1911	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	7	36	theme	biological	1013:1022	arg1	profiles					1024:1031	functional biological profiles	1002:1031	functional biological profiles	1002:1031	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	0	37	theme	Bread	11:15	arg1	Beta-Glucans					33:44	Bread Yeast Cell Wall Beta-Glucans	11:44	Bread Yeast Cell Wall Beta-Glucans	11:44	Effects of Bread Yeast Cell Wall Beta-Glucans on Mice with Loperamide-Induced Constipation.					
31536448	7	38	theme	composition	986:996	arg1	data					1033:1036	the intestinal microbiota composition and functional biological profiles data	960:1036	the intestinal microbiota composition and functional biological profiles data	960:1036	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	7	39	theme	high-throughput	922:936	arg1	sequencing					938:947	high-throughput sequencing	922:947	high-throughput sequencing	922:947	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	8	40	theme	distal	1251:1256	arg1	colon					1258:1262	distal colon	1251:1262	distal colon	1251:1262	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	0	41	theme	Wall	28:31	arg1	Beta-Glucans					33:44	Bread Yeast Cell Wall Beta-Glucans	11:44	Bread Yeast Cell Wall Beta-Glucans	11:44	Effects of Bread Yeast Cell Wall Beta-Glucans on Mice with Loperamide-Induced Constipation.					
31536448	5	42	from	effect	591:596	arg1	mice					687:690	loperamide-induced constipation mice	655:690	loperamide-induced constipation mice	655:690	The aim of this study was to characterize the effect of β-glucans extracted from the bread yeast cell wall on loperamide-induced constipation mice.					
31536448	2	43	theme	Increasing	190:199	arg1	evidence					201:208	Increasing evidence	190:208	Increasing evidence	190:208	Increasing evidence indicates that long-term use of irritant laxatives causes serious side effects.					
31536448	8	44	theme	protein	1193:1199	arg1	expression					1164:1173	expression	1164:1173	expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2	1164:1246	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	8	44	theme	protein	1193:1199	arg1	expression					1062:1071	expression	1062:1071	expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT)	1062:1158	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	0	45	theme	Cell	23:26	arg1	Beta-Glucans					33:44	Bread Yeast Cell Wall Beta-Glucans	11:44	Bread Yeast Cell Wall Beta-Glucans	11:44	Effects of Bread Yeast Cell Wall Beta-Glucans on Mice with Loperamide-Induced Constipation.					
31536448	10	46	theme	group	1516:1520	arg1	closer					1550:1555	closer	1550:1555	closer	1550:1555	The intestinal microecological portion of the treatment group partially recovered and was closer to the normal group.					
31536448	10	46	theme	group	1516:1520	arg1	portion					1491:1497	The intestinal microecological portion	1460:1497	The intestinal microecological portion of the treatment group	1460:1520	The intestinal microecological portion of the treatment group partially recovered and was closer to the normal group.					
31536448	11	47	theme	TJP	1736:1738	arg1	express					1703:1709	the express	1699:1709	the express of neurotransmitters and TJP to recover intestinal epithelial mechanical barrier	1699:1790	This study showed that β-glucans can influence the intestinal microbiota and restore microecological balance to regulate the express of neurotransmitters and TJP to recover intestinal epithelial mechanical barrier.					
31536448	2	48	theme	laxatives	251:259	arg1	use					235:237	long-term use	225:237	long-term use of irritant laxatives	225:259	Increasing evidence indicates that long-term use of irritant laxatives causes serious side effects.					
31536448	7	49	theme	functional	1002:1011	arg1	profiles					1024:1031	functional biological profiles	1002:1031	functional biological profiles	1002:1031	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	8	50	theme	zonula	1217:1222	arg1	occludens-1					1224:1234	zonula occludens-1	1217:1234	zonula occludens-1	1217:1234	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	8	51	theme	substance	1126:1134	arg1	P					1136:1136	substance P	1126:1136	substance P	1126:1136	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	2	52	theme	irritant	242:249	arg1	laxatives					251:259	irritant laxatives	242:259	irritant laxatives	242:259	Increasing evidence indicates that long-term use of irritant laxatives causes serious side effects.					
31536448	6	53	theme	constipation	752:763	arg1	model					765:769	the constipation model	748:769	the constipation model	748:769	Forty mice were fed with loperamide (10 mg/kg) to make the constipation model and a diet supplemented with 2.5, 5, and 10 mg/kg β-glucan.					
31536448	1	54	theme	common	110:115	arg1	disorder					134:141	a common gastrointestinal disorder	108:141	a common gastrointestinal disorder characterized by changes in intestinal habits	108:187	Constipation is a common gastrointestinal disorder characterized by changes in intestinal habits.					
31536448	1	54	theme	common	110:115	arg1	Constipation					92:103	Constipation	92:103	Constipation	92:103	Constipation is a common gastrointestinal disorder characterized by changes in intestinal habits.					
31536448	0	55	theme	Beta-Glucans	33:44	arg1	Effects					0:6	Effects	0:6	Effects of Bread Yeast Cell Wall Beta-Glucans on Mice with Loperamide-Induced Constipation	0:89	Effects of Bread Yeast Cell Wall Beta-Glucans on Mice with Loperamide-Induced Constipation.					
31536448	3	56	theme	patients	318:325	arg1	%					313:313	more than 50%	301:313	more than 50% of patients	301:325	Meanwhile, more than 50% of patients are dissatisfied with sense of use of non-prescriptional laxatives.					
31536448	3	56	theme	patients	318:325	arg1	Meanwhile					290:298	Meanwhile	290:298	Meanwhile	290:298	Meanwhile, more than 50% of patients are dissatisfied with sense of use of non-prescriptional laxatives.					
31536448	3	56	theme	patients	318:325	arg1	patients					318:325	patients	318:325	patients	318:325	Meanwhile, more than 50% of patients are dissatisfied with sense of use of non-prescriptional laxatives.					
31536448	5	57	theme	study	561:565	arg1	aim					549:551	The aim	545:551	The aim of this study	545:565	The aim of this study was to characterize the effect of β-glucans extracted from the bread yeast cell wall on loperamide-induced constipation mice.					
31536448	1	58	theme	gastrointestinal	117:132	arg1	disorder					134:141	a common gastrointestinal disorder	108:141	a common gastrointestinal disorder characterized by changes in intestinal habits	108:187	Constipation is a common gastrointestinal disorder characterized by changes in intestinal habits.					
31536448	1	58	theme	gastrointestinal	117:132	arg1	Constipation					92:103	Constipation	92:103	Constipation	92:103	Constipation is a common gastrointestinal disorder characterized by changes in intestinal habits.					
31536448	11	59	theme	epithelial	1762:1771	arg1	barrier					1784:1790	intestinal epithelial mechanical barrier	1751:1790	intestinal epithelial mechanical barrier	1751:1790	This study showed that β-glucans can influence the intestinal microbiota and restore microecological balance to regulate the express of neurotransmitters and TJP to recover intestinal epithelial mechanical barrier.					
31536448	11	60	theme	microecological	1663:1677	arg1	balance					1679:1685	microecological balance	1663:1685	microecological balance	1663:1685	This study showed that β-glucans can influence the intestinal microbiota and restore microecological balance to regulate the express of neurotransmitters and TJP to recover intestinal epithelial mechanical barrier.					
31536448	7	61	theme	profiles	1024:1031	arg1	data					1033:1036	the intestinal microbiota composition and functional biological profiles data	960:1036	the intestinal microbiota composition and functional biological profiles data	960:1036	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	2	62	theme	long-term	225:233	arg1	use					235:237	long-term use	225:237	long-term use of irritant laxatives	225:259	Increasing evidence indicates that long-term use of irritant laxatives causes serious side effects.					
31536448	5	63	theme	β-glucans	601:609	arg1	effect					591:596	the effect	587:596	the effect of β-glucans extracted from the bread yeast cell wall on loperamide-induced constipation mice	587:690	The aim of this study was to characterize the effect of β-glucans extracted from the bread yeast cell wall on loperamide-induced constipation mice.					
31536448	7	64	theme	mice	900:903	arg1	function					888:895	intestinal transit function	869:895	intestinal transit function of mice	869:903	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	7	64	theme	mice	900:903	arg1	frequency					858:866	the defecation frequency	843:866	the defecation frequency	843:866	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	9	65	from	expression	1411:1420	arg1	mice					1454:1457	mice	1454:1457	mice	1454:1457	β-glucans significantly enhanced intestinal motility and provided a possibility to regulate the expression of neurotransmitters and TJP in mice.					
31536448	4	66	theme	various	512:518	arg1	effects					536:542	various pharmacological effects	512:542	various pharmacological effects	512:542	β-glucans are natural polysaccharides widely found in yeast, fungus, and plants, which have been reported to exhibit various pharmacological effects.					
31536448	3	67	with	dissatisfied	331:342	arg1	sense					349:353	sense	349:353	sense of use of non-prescriptional laxatives	349:392	Meanwhile, more than 50% of patients are dissatisfied with sense of use of non-prescriptional laxatives.					
31536448	7	68	theme	intestinal	964:973	arg1	composition					986:996	the intestinal microbiota composition	960:996	the intestinal microbiota composition	960:996	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	8	69	theme	molecular	1293:1301	arg1	mechanisms					1303:1312	the possible molecular mechanisms	1280:1312	the possible molecular mechanisms	1280:1312	Meanwhile, we detected expression of neurotransmitters including acetylcholinesterase, substance P, and serotonin (5-HT) and expression of tight junction protein (TJP) including zonula occludens-1 and mucin-2 in distal colon to characterize the possible molecular mechanisms.					
31536448	7	70	theme	transit	880:886	arg1	function					888:895	intestinal transit function	869:895	intestinal transit function of mice	869:903	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	7	70	theme	transit	880:886	arg1	frequency					858:866	the defecation frequency	843:866	the defecation frequency	843:866	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	12	71	theme	active	1841:1846	arg1	supplement					1860:1869	an active nutritional supplement	1838:1869	an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis	1838:1979	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	12	71	theme	active	1841:1846	arg1	β-glucans					1811:1819	β-glucans	1811:1819	β-glucans	1811:1819	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	11	72	theme	mechanical	1773:1782	arg1	barrier					1784:1790	intestinal epithelial mechanical barrier	1751:1790	intestinal epithelial mechanical barrier	1751:1790	This study showed that β-glucans can influence the intestinal microbiota and restore microecological balance to regulate the express of neurotransmitters and TJP to recover intestinal epithelial mechanical barrier.					
31536448	2	73	theme	side	276:279	arg1	effects					281:287	serious side effects	268:287	serious side effects	268:287	Increasing evidence indicates that long-term use of irritant laxatives causes serious side effects.					
31536448	7	74	theme	microbiota	975:984	arg1	composition					986:996	the intestinal microbiota composition	960:996	the intestinal microbiota composition	960:996	We assessed the defecation frequency, intestinal transit function of mice, as well as used high-throughput sequencing to analyze the intestinal microbiota composition and functional biological profiles data.					
31536448	12	75	contain	have	1935:1938	arg2	constipation					1940:1951	constipation complications and dysbiosis	1940:1979	constipation	1940:1951	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	12	75	contain	have	1935:1938	arg1	patients					1922:1929	patients	1922:1929	patients who have constipation complications and dysbiosis	1922:1979	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	12	75	contain	have	1935:1938	arg2	dysbiosis					1971:1979	dysbiosis	1971:1979	dysbiosis	1971:1979	We suggested that β-glucans could be used as an active nutritional supplement to protect the damaged intestinal barrier and help patients who have constipation complications and dysbiosis.					
31536448	11	76	theme	intestinal	1629:1638	arg1	microbiota					1640:1649	the intestinal microbiota	1625:1649	the intestinal microbiota	1625:1649	This study showed that β-glucans can influence the intestinal microbiota and restore microecological balance to regulate the express of neurotransmitters and TJP to recover intestinal epithelial mechanical barrier.					
31536448	2	77	theme	serious	268:274	arg1	effects					281:287	serious side effects	268:287	serious side effects	268:287	Increasing evidence indicates that long-term use of irritant laxatives causes serious side effects.					
31536448	5	78	theme	cell	642:645	arg1	wall					647:650	the bread yeast cell wall	626:650	the bread yeast cell wall	626:650	The aim of this study was to characterize the effect of β-glucans extracted from the bread yeast cell wall on loperamide-induced constipation mice.					
31536448	9	79	theme	neurotransmitters	1425:1441	arg1	expression					1411:1420	the expression	1407:1420	the expression of neurotransmitters and TJP in mice	1407:1457	β-glucans significantly enhanced intestinal motility and provided a possibility to regulate the expression of neurotransmitters and TJP in mice.					
30008382	5	0	with	treatment	878:886	arg1	enzymes					906:912	ligninolytic enzymes	893:912	ligninolytic enzymes	893:912	As sediment oil concentrations decreased following treatment with ligninolytic enzymes, the microbial population was enriched with hydrocarbon-degrading species.					
30008382	1	1	theme	crude	175:179	arg1	oil					181:183	crude and weathered crude oil	155:183	crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes	155:273	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	6	2	theme	microbial	1067:1075	arg1	degradation					1077:1087	microbial degradation	1067:1087	microbial degradation	1067:1087	This trend demonstrates that the oil fractions initially not bioavailable for microbial degradation, were subsequently released to the sediment via catalytic conversion with laccase and manganese peroxidase, and the oil continues to be biodegraded by microbial populations.					
30008382	3	3	theme	saturates	683:691	arg1	hydrocarbons					606:617	total petroleum hydrocarbons	590:617	total petroleum hydrocarbons (TPH)	590:623	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	3	theme	saturates	683:691	arg1	PAHs					660:663	PAHs	660:663	PAHs	660:663	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	3	theme	saturates	683:691	arg1	fractions					670:678	fractions	670:678	fractions of saturates, aromatics, resins and asphaltenes (SARA)	670:733	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	3	theme	saturates	683:691	arg1	hydrocarbons					646:657	polycyclic aromatic hydrocarbons	626:657	polycyclic aromatic hydrocarbons (PAHs)	626:664	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	3	theme	saturates	683:691	arg1	TPH					620:622	TPH	620:622	TPH	620:622	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	2	4	theme	crude	378:382	arg1	extracts					391:398	crude enzyme extracts	378:398	crude enzyme extracts	378:398	Changes in the chemical composition of the oil were monitored in crude enzyme extracts, as well as a sediment matrix, for as long as 70 days.					
30008382	1	5	theme	in	293:294	arg1	microcosms					301:310	in situ microcosms	293:310	in situ microcosms	293:310	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	0	6	theme	calcium-alginate	112:127	arg1	beads					129:133	calcium-alginate beads	112:133	calcium-alginate beads	112:133	Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads.					
30008382	4	7	theme	most	758:761	arg1	fractions					775:783	most TPH and PAH fractions	758:783	most TPH and PAH fractions	758:783	As the oil weathered, most TPH and PAH fractions showed a rapid decrease in concentration.					
30008382	4	8	theme	rapid	794:798	arg1	decrease					800:807	a rapid decrease	792:807	a rapid decrease in concentration	792:824	As the oil weathered, most TPH and PAH fractions showed a rapid decrease in concentration.					
30008382	4	8	theme	rapid	794:798	arg1	weathered					747:755	the oil weathered	739:755	the oil weathered	739:755	As the oil weathered, most TPH and PAH fractions showed a rapid decrease in concentration.					
30008382	4	9	theme	oil	743:745	arg1	decrease					800:807	a rapid decrease	792:807	a rapid decrease in concentration	792:824	As the oil weathered, most TPH and PAH fractions showed a rapid decrease in concentration.					
30008382	4	9	theme	oil	743:745	arg1	weathered					747:755	the oil weathered	739:755	the oil weathered	739:755	As the oil weathered, most TPH and PAH fractions showed a rapid decrease in concentration.					
30008382	1	10	theme	oil	181:183	arg1	degradation					140:150	The degradation	136:150	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes	136:273	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	3	11	theme	asphaltenes	716:726	arg1	hydrocarbons					606:617	total petroleum hydrocarbons	590:617	total petroleum hydrocarbons (TPH)	590:623	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	11	theme	asphaltenes	716:726	arg1	PAHs					660:663	PAHs	660:663	PAHs	660:663	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	11	theme	asphaltenes	716:726	arg1	fractions					670:678	fractions	670:678	fractions of saturates, aromatics, resins and asphaltenes (SARA)	670:733	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	11	theme	asphaltenes	716:726	arg1	hydrocarbons					646:657	polycyclic aromatic hydrocarbons	626:657	polycyclic aromatic hydrocarbons (PAHs)	626:664	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	11	theme	asphaltenes	716:726	arg1	TPH					620:622	TPH	620:622	TPH	620:622	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	5	12	theme	hydrocarbon-degrading	958:978	arg1	species					980:986	hydrocarbon-degrading species	958:986	hydrocarbon-degrading species	958:986	As sediment oil concentrations decreased following treatment with ligninolytic enzymes, the microbial population was enriched with hydrocarbon-degrading species.					
30008382	2	13	theme	enzyme	384:389	arg1	extracts					391:398	crude enzyme extracts	378:398	crude enzyme extracts	378:398	Changes in the chemical composition of the oil were monitored in crude enzyme extracts, as well as a sediment matrix, for as long as 70 days.					
30008382	1	14	theme	crude	155:159	arg1	oil					181:183	crude and weathered crude oil	155:183	crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes	155:273	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	5	15	theme	ligninolytic	893:904	arg1	enzymes					906:912	ligninolytic enzymes	893:912	ligninolytic enzymes	893:912	As sediment oil concentrations decreased following treatment with ligninolytic enzymes, the microbial population was enriched with hydrocarbon-degrading species.					
30008382	3	16	theme	resins	705:710	arg1	hydrocarbons					606:617	total petroleum hydrocarbons	590:617	total petroleum hydrocarbons (TPH)	590:623	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	16	theme	resins	705:710	arg1	PAHs					660:663	PAHs	660:663	PAHs	660:663	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	16	theme	resins	705:710	arg1	fractions					670:678	fractions	670:678	fractions of saturates, aromatics, resins and asphaltenes (SARA)	670:733	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	16	theme	resins	705:710	arg1	hydrocarbons					646:657	polycyclic aromatic hydrocarbons	626:657	polycyclic aromatic hydrocarbons (PAHs)	626:664	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	16	theme	resins	705:710	arg1	TPH					620:622	TPH	620:622	TPH	620:622	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	5	17	theme	sediment	830:837	arg1	concentrations					843:856	sediment oil concentrations	830:856	sediment oil concentrations	830:856	As sediment oil concentrations decreased following treatment with ligninolytic enzymes, the microbial population was enriched with hydrocarbon-degrading species.					
30008382	4	18	from	decrease	800:807	arg1	concentration					812:824	concentration	812:824	concentration	812:824	As the oil weathered, most TPH and PAH fractions showed a rapid decrease in concentration.					
30008382	5	19	theme	microbial	919:927	arg1	population					929:938	the microbial population	915:938	the microbial population	915:938	As sediment oil concentrations decreased following treatment with ligninolytic enzymes, the microbial population was enriched with hydrocarbon-degrading species.					
30008382	5	20	theme	oil	839:841	arg1	concentrations					843:856	sediment oil concentrations	830:856	sediment oil concentrations	830:856	As sediment oil concentrations decreased following treatment with ligninolytic enzymes, the microbial population was enriched with hydrocarbon-degrading species.					
30008382	0	21	theme	Enhanced	0:7	arg1	biodegradation					9:22	Enhanced biodegradation	0:22	Enhanced biodegradation of sediment-bound	0:40	Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads.					
30008382	6	22	theme	manganese	1175:1183	arg1	peroxidase					1185:1194	manganese peroxidase	1175:1194	manganese peroxidase	1175:1194	This trend demonstrates that the oil fractions initially not bioavailable for microbial degradation, were subsequently released to the sediment via catalytic conversion with laccase and manganese peroxidase, and the oil continues to be biodegraded by microbial populations.					
30008382	0	23	theme	sediment-bound	27:40	arg1	biodegradation					9:22	Enhanced biodegradation	0:22	Enhanced biodegradation of sediment-bound	0:40	Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads.					
30008382	2	24	theme	sediment	414:421	arg1	matrix					423:428	a sediment matrix	412:428	a sediment matrix	412:428	Changes in the chemical composition of the oil were monitored in crude enzyme extracts, as well as a sediment matrix, for as long as 70 days.					
30008382	3	25	theme	Compound-specific	455:471	arg1	effects					473:479	Compound-specific effects	455:479	Compound-specific effects of ligninolytic enzymes applied to the sediments	455:528	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	2	26	theme	chemical	328:335	arg1	composition					337:347	the chemical composition	324:347	the chemical composition of the oil	324:358	Changes in the chemical composition of the oil were monitored in crude enzyme extracts, as well as a sediment matrix, for as long as 70 days.					
30008382	6	27	theme	microbial	1240:1248	arg1	populations					1250:1260	microbial populations	1240:1260	microbial populations	1240:1260	This trend demonstrates that the oil fractions initially not bioavailable for microbial degradation, were subsequently released to the sediment via catalytic conversion with laccase and manganese peroxidase, and the oil continues to be biodegraded by microbial populations.					
30008382	6	28	theme	bioavailable	1050:1061	arg1	fractions					1026:1034	the oil fractions	1018:1034	the oil fractions	1018:1034	This trend demonstrates that the oil fractions initially not bioavailable for microbial degradation, were subsequently released to the sediment via catalytic conversion with laccase and manganese peroxidase, and the oil continues to be biodegraded by microbial populations.					
30008382	3	29	theme	hydrocarbons	606:617	arg1	concentration					573:585	concentration	573:585	concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA)	573:733	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	1	30	theme	calcium-alginate	224:239	arg1	enzymes					267:273	crude and calcium-alginate encapsulated ligninolytic enzymes	214:273	crude and calcium-alginate encapsulated ligninolytic enzymes	214:273	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	3	31	from	changes	562:568	arg1	concentration					573:585	concentration	573:585	concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA)	573:733	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	32	theme	aromatic	637:644	arg1	PAHs					660:663	PAHs	660:663	PAHs	660:663	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	32	theme	aromatic	637:644	arg1	hydrocarbons					646:657	polycyclic aromatic hydrocarbons	626:657	polycyclic aromatic hydrocarbons (PAHs)	626:664	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	1	33	theme	encapsulated	241:252	arg1	enzymes					267:273	crude and calcium-alginate encapsulated ligninolytic enzymes	214:273	crude and calcium-alginate encapsulated ligninolytic enzymes	214:273	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	3	34	theme	hydrocarbons	646:657	arg1	concentration					573:585	concentration	573:585	concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA)	573:733	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	35	theme	total	590:594	arg1	hydrocarbons					606:617	total petroleum hydrocarbons	590:617	total petroleum hydrocarbons (TPH)	590:623	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	35	theme	total	590:594	arg1	TPH					620:622	TPH	620:622	TPH	620:622	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	6	36	with	conversion	1147:1156	arg1	laccase					1163:1169	laccase	1163:1169	laccase	1163:1169	This trend demonstrates that the oil fractions initially not bioavailable for microbial degradation, were subsequently released to the sediment via catalytic conversion with laccase and manganese peroxidase, and the oil continues to be biodegraded by microbial populations.					
30008382	6	36	with	conversion	1147:1156	arg1	peroxidase					1185:1194	manganese peroxidase	1175:1194	manganese peroxidase	1175:1194	This trend demonstrates that the oil fractions initially not bioavailable for microbial degradation, were subsequently released to the sediment via catalytic conversion with laccase and manganese peroxidase, and the oil continues to be biodegraded by microbial populations.					
30008382	3	37	theme	enzymes	497:503	arg1	effects					473:479	Compound-specific effects	455:479	Compound-specific effects of ligninolytic enzymes applied to the sediments	455:528	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	0	38	theme	crude	60:64	arg1	oil					66:68	crude oil	60:68	crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads	60:133	Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads.					
30008382	3	39	theme	fractions	670:678	arg1	concentration					573:585	concentration	573:585	concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA)	573:733	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	40	theme	petroleum	596:604	arg1	hydrocarbons					606:617	total petroleum hydrocarbons	590:617	total petroleum hydrocarbons (TPH)	590:623	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	40	theme	petroleum	596:604	arg1	TPH					620:622	TPH	620:622	TPH	620:622	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	2	41	from	Changes	313:319	arg1	composition					337:347	the chemical composition	324:347	the chemical composition of the oil	324:358	Changes in the chemical composition of the oil were monitored in crude enzyme extracts, as well as a sediment matrix, for as long as 70 days.					
30008382	4	42	theme	TPH	763:765	arg1	fractions					775:783	most TPH and PAH fractions	758:783	most TPH and PAH fractions	758:783	As the oil weathered, most TPH and PAH fractions showed a rapid decrease in concentration.					
30008382	6	43	theme	catalytic	1137:1145	arg1	conversion					1147:1156	catalytic conversion	1137:1156	catalytic conversion with laccase and manganese peroxidase	1137:1194	This trend demonstrates that the oil fractions initially not bioavailable for microbial degradation, were subsequently released to the sediment via catalytic conversion with laccase and manganese peroxidase, and the oil continues to be biodegraded by microbial populations.					
30008382	1	44	theme	ligninolytic	254:265	arg1	enzymes					267:273	crude and calcium-alginate encapsulated ligninolytic enzymes	214:273	crude and calcium-alginate encapsulated ligninolytic enzymes	214:273	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	3	45	theme	polycyclic	626:635	arg1	PAHs					660:663	PAHs	660:663	PAHs	660:663	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	45	theme	polycyclic	626:635	arg1	hydrocarbons					646:657	polycyclic aromatic hydrocarbons	626:657	polycyclic aromatic hydrocarbons (PAHs)	626:664	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	0	46	with	oil	66:68	arg1	enzymes					88:94	ligninolytic enzymes	75:94	ligninolytic enzymes encapsulated in calcium-alginate beads	75:133	Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads.					
30008382	2	47	theme	oil	356:358	arg1	composition					337:347	the chemical composition	324:347	the chemical composition of the oil	324:358	Changes in the chemical composition of the oil were monitored in crude enzyme extracts, as well as a sediment matrix, for as long as 70 days.					
30008382	1	48	theme	enzymes	267:273	arg1	application					199:209	the application	195:209	the application of crude and calcium-alginate encapsulated ligninolytic enzymes	195:273	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	0	49	theme	ligninolytic	75:86	arg1	enzymes					88:94	ligninolytic enzymes	75:94	ligninolytic enzymes encapsulated in calcium-alginate beads	75:133	Enhanced biodegradation of sediment-bound heavily weathered crude oil with ligninolytic enzymes encapsulated in calcium-alginate beads.					
30008382	1	50	dep	in	293:294	arg1	situ					296:299	situ	296:299	situ	296:299	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	4	51	theme	PAH	771:773	arg1	fractions					775:783	most TPH and PAH fractions	758:783	most TPH and PAH fractions	758:783	As the oil weathered, most TPH and PAH fractions showed a rapid decrease in concentration.					
30008382	1	52	theme	crude	214:218	arg1	enzymes					267:273	crude and calcium-alginate encapsulated ligninolytic enzymes	214:273	crude and calcium-alginate encapsulated ligninolytic enzymes	214:273	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
30008382	3	53	theme	ligninolytic	484:495	arg1	enzymes					497:503	ligninolytic enzymes	484:503	ligninolytic enzymes applied to the sediments	484:528	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	6	54	theme	oil	1022:1024	arg1	fractions					1026:1034	the oil fractions	1018:1034	the oil fractions	1018:1034	This trend demonstrates that the oil fractions initially not bioavailable for microbial degradation, were subsequently released to the sediment via catalytic conversion with laccase and manganese peroxidase, and the oil continues to be biodegraded by microbial populations.					
30008382	3	55	theme	aromatics	694:702	arg1	hydrocarbons					606:617	total petroleum hydrocarbons	590:617	total petroleum hydrocarbons (TPH)	590:623	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	55	theme	aromatics	694:702	arg1	PAHs					660:663	PAHs	660:663	PAHs	660:663	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	55	theme	aromatics	694:702	arg1	fractions					670:678	fractions	670:678	fractions of saturates, aromatics, resins and asphaltenes (SARA)	670:733	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	55	theme	aromatics	694:702	arg1	hydrocarbons					646:657	polycyclic aromatic hydrocarbons	626:657	polycyclic aromatic hydrocarbons (PAHs)	626:664	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	3	55	theme	aromatics	694:702	arg1	TPH					620:622	TPH	620:622	TPH	620:622	Compound-specific effects of ligninolytic enzymes applied to the sediments were observed over time through changes in concentration of total petroleum hydrocarbons (TPH), polycyclic aromatic hydrocarbons (PAHs) and fractions of saturates, aromatics, resins and asphaltenes (SARA).					
30008382	1	56	theme	weathered	165:173	arg1	oil					181:183	crude and weathered crude oil	155:183	crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes	155:273	The degradation of crude and weathered crude oil following the application of crude and calcium-alginate encapsulated ligninolytic enzymes was studied using in situ microcosms.					
31347237	4	0	theme	mechanical	899:908	arg1	properties					910:919	mechanical properties	899:919	mechanical properties	899:919	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	1	1	theme	functional	181:190	arg1	hydrogels					155:163	Peptide-based supramolecular hydrogels	126:163	Peptide-based supramolecular hydrogels	126:163	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	1	1	theme	functional	181:190	arg1	materials					192:200	functional materials	181:200	functional materials in tissue engineering, axonal regeneration, and controlled drug delivery	181:273	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	4	2	theme	biodegradable	803:815	arg1	chitosan					826:833	chitosan	826:833	chitosan	826:833	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	4	2	theme	biodegradable	803:815	arg1	polymer					817:823	a biocompatible and biodegradable polymer	783:823	a biocompatible and biodegradable polymer	783:823	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	0	3	theme	Multifunctional	81:95	arg1	Material					116:123	a New Multifunctional, Bio-Inspired Soft Material	75:123	Material	116:123	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	7	4	contain	have	1205:1208	arg1	hydrogel					1184:1191	The composite hydrogel	1170:1191	The composite hydrogel	1170:1191	The composite hydrogel is found to have notable antibacterial activity, which stems from the inherent antibacterial properties of chitosan.					
31347237	7	4	contain	have	1205:1208	arg2	activity					1232:1239	notable antibacterial activity	1210:1239	notable antibacterial activity	1210:1239	The composite hydrogel is found to have notable antibacterial activity, which stems from the inherent antibacterial properties of chitosan.					
31347237	2	5	dep	based	305:309	arg1	ligand					298:303	Arg-Gly-Asp (RGD) ligand	280:303	Arg-Gly-Asp (RGD) ligand	280:303	The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion.					
31347237	0	6	theme	Soft	111:114	arg1	Material					116:123	a New Multifunctional, Bio-Inspired Soft Material	75:123	Material	116:123	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	7	7	theme	composite	1174:1182	arg1	hydrogel					1184:1191	The composite hydrogel	1170:1191	The composite hydrogel	1170:1191	The composite hydrogel is found to have notable antibacterial activity, which stems from the inherent antibacterial properties of chitosan.					
31347237	1	8	from	materials	192:200	arg1	delivery					266:273	controlled drug delivery	250:273	controlled drug delivery	250:273	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	1	8	from	materials	192:200	arg1	engineering					212:222	tissue engineering	205:222	tissue engineering	205:222	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	1	8	from	materials	192:200	arg1	regeneration					232:243	axonal regeneration	225:243	axonal regeneration	225:243	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	2	9	theme	supramolecular	311:324	arg1	gels					326:329	The Arg-Gly-Asp (RGD) ligand based supramolecular gels	276:329	The Arg-Gly-Asp (RGD) ligand based supramolecular gels	276:329	The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion.					
31347237	0	10	theme	Bio-Inspired	98:109	arg1	Material					116:123	a New Multifunctional, Bio-Inspired Soft Material	75:123	Material	116:123	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	3	11	theme	in	573:574	arg1	culture					587:593	in vitro cell culture	573:593	in vitro cell culture	573:593	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	2	12	theme	based	305:309	arg1	gels					326:329	The Arg-Gly-Asp (RGD) ligand based supramolecular gels	276:329	The Arg-Gly-Asp (RGD) ligand based supramolecular gels	276:329	The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion.					
31347237	4	13	theme	Fmoc-RGD	723:730	arg1	hydrogelator					732:743	the Fmoc-RGD hydrogelator	719:743	the Fmoc-RGD hydrogelator	719:743	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	4	14	theme	enhanced	875:882	arg1	rate					893:896	enhanced gelation rate	875:896	enhanced gelation rate	875:896	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	3	15	theme	long-range	548:557	arg1	stability					559:567	long-range stability	548:567	long-range stability	548:567	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	4	16	from	durability	685:694	arg1	media					712:716	cell culture media	699:716	cell culture media	699:716	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	2	17	theme	cell	410:413	arg1	adhesion					415:422	cell adhesion	410:422	cell adhesion	410:422	The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion.					
31347237	9	18	theme	supramolecular-polymer	1523:1544	arg1	attributes					1500:1509	the attributes	1496:1509	the attributes of both the supramolecular-polymer (Fmoc-RGD) and the covalent-polymer (chitosan) component	1496:1601	Therefore, the authors' approach harnesses the attributes of both the supramolecular-polymer (Fmoc-RGD) and the covalent-polymer (chitosan) component, resulting in a composite hydrogel with excellent potential.					
31347237	7	19	theme	notable	1210:1216	arg1	activity					1232:1239	notable antibacterial activity	1210:1239	notable antibacterial activity	1210:1239	The composite hydrogel is found to have notable antibacterial activity, which stems from the inherent antibacterial properties of chitosan.					
31347237	7	20	theme	inherent	1263:1270	arg1	properties					1286:1295	the inherent antibacterial properties	1259:1295	the inherent antibacterial properties of chitosan	1259:1307	The composite hydrogel is found to have notable antibacterial activity, which stems from the inherent antibacterial properties of chitosan.					
31347237	1	21	used	utilized	169:176	arg2	materials					192:200	functional materials	181:200	functional materials in tissue engineering, axonal regeneration, and controlled drug delivery	181:273	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	1	21	used	utilized	169:176	arg2	hydrogels					155:163	Peptide-based supramolecular hydrogels	126:163	Peptide-based supramolecular hydrogels	126:163	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	1	22	theme	tissue	205:210	arg1	engineering					212:222	tissue engineering	205:222	tissue engineering	205:222	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	4	23	dep	rate	893:896	arg1	durability					936:945	durability	936:945	durability	936:945	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	8	24	theme	composite	1327:1335	arg1	able					1351:1354	able	1351:1354	able	1351:1354	Furthermore, the composite hydrogels are able to produce ultra-small, mono-dispersed, silver nanoparticles (AgNPs) arranged on the fiber axis.					
31347237	8	24	theme	composite	1327:1335	arg1	hydrogels					1337:1345	the composite hydrogels	1323:1345	the composite hydrogels	1323:1345	Furthermore, the composite hydrogels are able to produce ultra-small, mono-dispersed, silver nanoparticles (AgNPs) arranged on the fiber axis.					
31347237	9	25	theme	component	1593:1601	arg1	attributes					1500:1509	the attributes	1496:1509	the attributes of both the supramolecular-polymer (Fmoc-RGD) and the covalent-polymer (chitosan) component	1496:1601	Therefore, the authors' approach harnesses the attributes of both the supramolecular-polymer (Fmoc-RGD) and the covalent-polymer (chitosan) component, resulting in a composite hydrogel with excellent potential.					
31347237	4	26	from	properties	633:642	arg1	media					712:716	cell culture media	699:716	cell culture media	699:716	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	8	27	theme	fiber	1441:1445	arg1	axis					1447:1450	the fiber axis	1437:1450	the fiber axis	1437:1450	Furthermore, the composite hydrogels are able to produce ultra-small, mono-dispersed, silver nanoparticles (AgNPs) arranged on the fiber axis.					
31347237	6	28	theme	3D	1136:1137	arg1	cultures					1144:1151	2D and 3D cell cultures	1129:1151	2D and 3D cell cultures	1129:1151	The utilization of the Fmoc-RGD/chitosan composite hydrogel as a scaffold for 2D and 3D cell cultures is demonstrated.					
31347237	3	29	theme	RGD-based	442:450	arg1	hydrogels					467:475	several RGD-based supramolecular hydrogels	434:475	several RGD-based supramolecular hydrogels	434:475	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	4	30	theme	gelation	884:891	arg1	rate					893:896	enhanced gelation rate	875:896	enhanced gelation rate	875:896	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	2	31	theme	immense	336:342	arg1	potential					344:352	immense potential	336:352	immense potential	336:352	The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion.					
31347237	3	32	theme	supramolecular	452:465	arg1	hydrogels					467:475	several RGD-based supramolecular hydrogels	434:475	several RGD-based supramolecular hydrogels	434:475	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	0	33	theme	Polymer	36:42	arg1	Composite					0:8	Composite	0:8	Composite of Peptide-Supramolecular Polymer and Covalent Polymer	0:63	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	0	33	theme	Polymer	36:42	arg1	Polymer					36:42	Peptide-Supramolecular Polymer	13:42	Peptide-Supramolecular Polymer	13:42	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	0	33	theme	Polymer	36:42	arg1	Polymer					57:63	Covalent Polymer	48:63	Covalent Polymer	48:63	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	8	34	dep	ultra-small	1367:1377	arg1	silver					1396:1401	silver	1396:1401	silver	1396:1401	Furthermore, the composite hydrogels are able to produce ultra-small, mono-dispersed, silver nanoparticles (AgNPs) arranged on the fiber axis.					
31347237	8	34	dep	ultra-small	1367:1377	arg1	mono-dispersed					1380:1393	mono-dispersed	1380:1393	mono-dispersed	1380:1393	Furthermore, the composite hydrogels are able to produce ultra-small, mono-dispersed, silver nanoparticles (AgNPs) arranged on the fiber axis.					
31347237	8	35	theme	ultra-small	1367:1377	arg1	AgNPs					1418:1422	AgNPs	1418:1422	AgNPs	1418:1422	Furthermore, the composite hydrogels are able to produce ultra-small, mono-dispersed, silver nanoparticles (AgNPs) arranged on the fiber axis.					
31347237	8	35	theme	ultra-small	1367:1377	arg1	nanoparticles					1403:1415	ultra-small, mono-dispersed, silver nanoparticles	1367:1415	ultra-small, mono-dispersed, silver nanoparticles (AgNPs) arranged on the fiber axis	1367:1450	Furthermore, the composite hydrogels are able to produce ultra-small, mono-dispersed, silver nanoparticles (AgNPs) arranged on the fiber axis.					
31347237	5	36	theme	composite	999:1007	arg1	hydrogels					1009:1017	both Fmoc-RGD and Fmoc-RGD/chitosan composite hydrogels	963:1017	hydrogels	1009:1017	Interestingly, both Fmoc-RGD and Fmoc-RGD/chitosan composite hydrogels exhibit thixotropic properties.					
31347237	1	37	theme	axonal	225:230	arg1	regeneration					232:243	axonal regeneration	225:243	axonal regeneration	225:243	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	0	38	theme	Peptide-Supramolecular	13:34	arg1	Polymer					36:42	Peptide-Supramolecular Polymer	13:42	Peptide-Supramolecular Polymer	13:42	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	5	39	theme	Fmoc-RGD/chitosan	981:997	arg1	hydrogels					1009:1017	both Fmoc-RGD and Fmoc-RGD/chitosan composite hydrogels	963:1017	hydrogels	1009:1017	Interestingly, both Fmoc-RGD and Fmoc-RGD/chitosan composite hydrogels exhibit thixotropic properties.					
31347237	3	40	theme	cell	582:585	arg1	culture					587:593	in vitro cell culture	573:593	in vitro cell culture	573:593	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	4	41	theme	culture	704:710	arg1	media					712:716	cell culture media	699:716	cell culture media	699:716	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	0	42	theme	Covalent	48:55	arg1	Polymer					57:63	Covalent Polymer	48:63	Covalent Polymer	48:63	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	4	43	theme	gels	670:673	arg1	durability					685:694	their durability	679:694	their durability in cell culture media	679:716	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	4	43	theme	gels	670:673	arg1	properties					633:642	the mechanical properties	618:642	the mechanical properties of these tripeptide-based gels	618:673	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	3	44	theme	several	434:440	arg1	hydrogels					467:475	several RGD-based supramolecular hydrogels	434:475	several RGD-based supramolecular hydrogels	434:475	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	7	45	theme	antibacterial	1218:1230	arg1	activity					1232:1239	notable antibacterial activity	1210:1239	notable antibacterial activity	1210:1239	The composite hydrogel is found to have notable antibacterial activity, which stems from the inherent antibacterial properties of chitosan.					
31347237	4	46	theme	biocompatible	785:797	arg1	chitosan					826:833	chitosan	826:833	chitosan	826:833	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	4	46	theme	biocompatible	785:797	arg1	polymer					817:823	a biocompatible and biodegradable polymer	783:823	a biocompatible and biodegradable polymer	783:823	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	9	47	with	hydrogel	1629:1636	arg1	potential					1653:1661	excellent potential	1643:1661	excellent potential	1643:1661	Therefore, the authors' approach harnesses the attributes of both the supramolecular-polymer (Fmoc-RGD) and the covalent-polymer (chitosan) component, resulting in a composite hydrogel with excellent potential.					
31347237	4	48	theme	tripeptide-based	653:668	arg1	gels					670:673	these tripeptide-based gels	647:673	these tripeptide-based gels	647:673	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	4	49	with	hydrogel	861:868	arg1	rate					893:896	enhanced gelation rate	875:896	enhanced gelation rate	875:896	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	4	49	with	hydrogel	861:868	arg1	properties					910:919	mechanical properties	899:919	mechanical properties	899:919	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	4	49	with	hydrogel	861:868	arg1	media					930:934	cell media	925:934	cell media	925:934	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	2	50	contain	have	331:334	arg2	potential					344:352	immense potential	336:352	immense potential	336:352	The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion.					
31347237	2	50	contain	have	331:334	arg1	gels					326:329	The Arg-Gly-Asp (RGD) ligand based supramolecular gels	276:329	The Arg-Gly-Asp (RGD) ligand based supramolecular gels	276:329	The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion.					
31347237	7	51	theme	chitosan	1300:1307	arg1	properties					1286:1295	the inherent antibacterial properties	1259:1295	the inherent antibacterial properties of chitosan	1259:1307	The composite hydrogel is found to have notable antibacterial activity, which stems from the inherent antibacterial properties of chitosan.					
31347237	7	52	theme	antibacterial	1272:1284	arg1	properties					1286:1295	the inherent antibacterial properties	1259:1295	the inherent antibacterial properties of chitosan	1259:1307	The composite hydrogel is found to have notable antibacterial activity, which stems from the inherent antibacterial properties of chitosan.					
31347237	5	53	theme	thixotropic	1027:1037	arg1	properties					1039:1048	thixotropic properties	1027:1048	thixotropic properties	1027:1048	Interestingly, both Fmoc-RGD and Fmoc-RGD/chitosan composite hydrogels exhibit thixotropic properties.					
31347237	1	54	theme	Peptide-based	126:138	arg1	hydrogels					155:163	Peptide-based supramolecular hydrogels	126:163	Peptide-based supramolecular hydrogels	126:163	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	1	54	theme	Peptide-based	126:138	arg1	materials					192:200	functional materials	181:200	functional materials in tissue engineering, axonal regeneration, and controlled drug delivery	181:273	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	0	55	theme	Polymer	57:63	arg1	Composite					0:8	Composite	0:8	Composite of Peptide-Supramolecular Polymer and Covalent Polymer	0:63	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	0	55	theme	Polymer	57:63	arg1	Polymer					36:42	Peptide-Supramolecular Polymer	13:42	Peptide-Supramolecular Polymer	13:42	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	0	55	theme	Polymer	57:63	arg1	Polymer					57:63	Covalent Polymer	48:63	Covalent Polymer	48:63	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	4	56	theme	cell	925:928	arg1	media					930:934	cell media	925:934	cell media	925:934	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	4	57	theme	composite	851:859	arg1	hydrogel					861:868	a composite hydrogel	849:868	a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability	849:945	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	3	58	theme	adequate	524:531	arg1	resilience					533:542	adequate resilience	524:542	adequate resilience	524:542	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	1	59	theme	supramolecular	140:153	arg1	hydrogels					155:163	Peptide-based supramolecular hydrogels	126:163	Peptide-based supramolecular hydrogels	126:163	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	1	59	theme	supramolecular	140:153	arg1	materials					192:200	functional materials	181:200	functional materials in tissue engineering, axonal regeneration, and controlled drug delivery	181:273	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	6	60	theme	hydrogel	1102:1109	arg1	utilization					1055:1065	The utilization	1051:1065	The utilization of the Fmoc-RGD/chitosan composite hydrogel as a scaffold for 2D and 3D cell cultures	1051:1151	The utilization of the Fmoc-RGD/chitosan composite hydrogel as a scaffold for 2D and 3D cell cultures is demonstrated.					
31347237	9	61	theme	covalent-polymer	1565:1580	arg1	component					1593:1601	the covalent-polymer (chitosan) component	1561:1601	the covalent-polymer (chitosan) component	1561:1601	Therefore, the authors' approach harnesses the attributes of both the supramolecular-polymer (Fmoc-RGD) and the covalent-polymer (chitosan) component, resulting in a composite hydrogel with excellent potential.					
31347237	9	62	theme	excellent	1643:1651	arg1	potential					1653:1661	excellent potential	1643:1661	excellent potential	1643:1661	Therefore, the authors' approach harnesses the attributes of both the supramolecular-polymer (Fmoc-RGD) and the covalent-polymer (chitosan) component, resulting in a composite hydrogel with excellent potential.					
31347237	1	63	theme	controlled	250:259	arg1	delivery					266:273	controlled drug delivery	250:273	controlled drug delivery	250:273	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	3	64	theme	stability	559:567	arg1	devoid					514:519	devoid	514:519	devoid	514:519	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	9	65	theme	composite	1619:1627	arg1	hydrogel					1629:1636	a composite hydrogel	1617:1636	a composite hydrogel with excellent potential	1617:1661	Therefore, the authors' approach harnesses the attributes of both the supramolecular-polymer (Fmoc-RGD) and the covalent-polymer (chitosan) component, resulting in a composite hydrogel with excellent potential.					
31347237	3	66	dep	in	573:574	arg1	vitro					576:580	vitro	576:580	vitro	576:580	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	1	67	theme	drug	261:264	arg1	delivery					266:273	controlled drug delivery	250:273	controlled drug delivery	250:273	Peptide-based supramolecular hydrogels are utilized as functional materials in tissue engineering, axonal regeneration, and controlled drug delivery.					
31347237	0	68	theme	New	77:79	arg1	Material					116:123	a New Multifunctional, Bio-Inspired Soft Material	75:123	Material	116:123	Composite of Peptide-Supramolecular Polymer and Covalent Polymer Comprises a New Multifunctional, Bio-Inspired Soft Material.					
31347237	2	69	theme	Arg-Gly-Asp	280:290	arg1	ligand					298:303	Arg-Gly-Asp (RGD) ligand	280:303	Arg-Gly-Asp (RGD) ligand	280:303	The Arg-Gly-Asp (RGD) ligand based supramolecular gels have immense potential in this respect, as this tripeptide is known to promote cell adhesion.					
31347237	6	70	theme	2D	1129:1130	arg1	cultures					1144:1151	2D and 3D cell cultures	1129:1151	2D and 3D cell cultures	1129:1151	The utilization of the Fmoc-RGD/chitosan composite hydrogel as a scaffold for 2D and 3D cell cultures is demonstrated.					
31347237	3	71	theme	resilience	533:542	arg1	devoid					514:519	devoid	514:519	devoid	514:519	Although several RGD-based supramolecular hydrogels have been reported, most of them are devoid of adequate resilience and long-range stability for in vitro cell culture.					
31347237	6	72	theme	cell	1139:1142	arg1	cultures					1144:1151	2D and 3D cell cultures	1129:1151	2D and 3D cell cultures	1129:1151	The utilization of the Fmoc-RGD/chitosan composite hydrogel as a scaffold for 2D and 3D cell cultures is demonstrated.					
31347237	6	73	theme	composite	1092:1100	arg1	hydrogel					1102:1109	the Fmoc-RGD/chitosan composite hydrogel	1070:1109	the Fmoc-RGD/chitosan composite hydrogel	1070:1109	The utilization of the Fmoc-RGD/chitosan composite hydrogel as a scaffold for 2D and 3D cell cultures is demonstrated.					
31347237	6	74	theme	Fmoc-RGD/chitosan	1074:1090	arg1	hydrogel					1102:1109	the Fmoc-RGD/chitosan composite hydrogel	1070:1109	the Fmoc-RGD/chitosan composite hydrogel	1070:1109	The utilization of the Fmoc-RGD/chitosan composite hydrogel as a scaffold for 2D and 3D cell cultures is demonstrated.					
31347237	4	75	theme	cell	699:702	arg1	media					712:716	cell culture media	699:716	cell culture media	699:716	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31347237	4	76	theme	mechanical	622:631	arg1	properties					633:642	the mechanical properties	618:642	the mechanical properties of these tripeptide-based gels	618:673	In a quest to improve the mechanical properties of these tripeptide-based gels and their durability in cell culture media, the Fmoc-RGD hydrogelator is non-covalently functionalized with a biocompatible and biodegradable polymer, chitosan, resulting in a composite hydrogel with enhanced gelation rate, mechanical properties and cell media durability.					
31708889	3	0	theme	Chinese	659:665	arg1	alligator					667:675	the Chinese alligator	655:675	the Chinese alligator	655:675	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	6	1	theme	gut	1241:1243	arg1	microbiome					1245:1254	a carnivore gut microbiome	1229:1254	a carnivore gut microbiome dominated by Fusobacteria	1229:1280	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	8	2	theme	host-gut	1676:1683	arg1	shifts					1723:1728	host-gut microbe symbioses to seasonal dietary shifts	1676:1728	host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states	1676:1811	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	3	3	from	cycles	622:627	arg1	alligator					667:675	the Chinese alligator	655:675	the Chinese alligator	655:675	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	8	4	theme	symbioses	1693:1701	arg1	shifts					1723:1728	host-gut microbe symbioses to seasonal dietary shifts	1676:1728	host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states	1676:1811	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	8	5	theme	functional	1650:1659	arg1	adaptations					1661:1671	the functional adaptations	1646:1671	the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states	1646:1811	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	3	6	from	feeding	632:638	arg1	alligator					667:675	the Chinese alligator	655:675	the Chinese alligator	655:675	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	8	7	theme	seasonal	1706:1713	arg1	shifts					1723:1728	host-gut microbe symbioses to seasonal dietary shifts	1676:1728	host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states	1676:1811	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	1	8	theme	Chinese	157:163	arg1	alligator					165:173	the Chinese alligator	153:173	the Chinese alligator (Alligator sinensis)	153:194	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	1	8	theme	Chinese	157:163	arg1	sinensis					186:193	Alligator sinensis	176:193	Alligator sinensis	176:193	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	1	8	theme	Chinese	157:163	arg1	model					223:227	an ideal and intriguing model	199:227	an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation	199:318	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	3	9	theme	microbial	713:721	arg1	community					723:731	a core gut microbial community	702:731	a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions	702:837	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	6	10	theme	active	1195:1200	arg1	alligators					1210:1219	active Chinese alligators	1195:1219	active Chinese alligators	1195:1219	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	3	11	theme	gut	541:543	arg1	communities					555:565	gut microbial communities	541:565	gut microbial communities	541:565	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	8	12	theme	shifts	1723:1728	arg1	adaptations					1661:1671	the functional adaptations	1646:1671	the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states	1646:1811	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	2	13	theme	alligators	507:516	arg1	microbiome					462:471	the gut microbiome	454:471	the gut microbiome of hibernating vs. active Chinese alligators	454:516	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	3	14	theme	core	704:707	arg1	community					723:731	a core gut microbial community	702:731	a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions	702:837	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	0	15	theme	Chinese	109:115	arg1	Alligator					117:125	the Chinese Alligator	105:125	the Chinese Alligator	105:125	Metagenomics Reveals Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator.					
31708889	6	16	theme	digestion	1474:1482	arg1	efficiency					1484:1493	host protein-rich food digestion efficiency	1451:1493	host protein-rich food digestion efficiency	1451:1493	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	4	17	theme	bacterial	901:909	arg1	taxa					911:914	bacterial taxa	901:914	bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans	901:980	During hibernation, there is an increase in the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans, which allows adaptation to winter fasting.					
31708889	3	18	from	alligator	667:675	arg1	cycles					622:627	seasonal cycles	613:627	seasonal cycles of feeding and fasting in the Chinese alligator	613:675	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	3	19	theme	seasonal	575:582	arg1	restructuring					584:596	seasonal restructuring	575:596	seasonal restructuring	575:596	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	7	20	theme	intestinal	1588:1597	arg1	immunity					1599:1606	intestinal immunity	1588:1606	intestinal immunity	1588:1606	In addition, seasonal variations in the expression of β-defensins play a protective role in intestinal immunity.					
31708889	4	21	dep	Bacteroides	933:943	arg1	e.g.					917:920	e.g.	917:920	e.g.	917:920	During hibernation, there is an increase in the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans, which allows adaptation to winter fasting.					
31708889	4	22	theme	genus	927:931	arg1	Bacteroides					933:943	the genus Bacteroides	923:943	the genus Bacteroides	923:943	During hibernation, there is an increase in the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans, which allows adaptation to winter fasting.					
31708889	0	23	theme	Microbiome	63:72	arg1	Adaptation					41:50	Seasonal Functional Adaptation	21:50	Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator	21:125	Metagenomics Reveals Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator.					
31708889	8	24	theme	extreme	1784:1790	arg1	states					1806:1811	extreme physiological states	1784:1811	extreme physiological states	1784:1811	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	1	25	theme	natural	133:139	arg1	hibernator					141:150	a natural hibernator	131:150	a natural hibernator	131:150	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	7	26	theme	β-defensins	1550:1560	arg1	expression					1536:1545	the expression	1532:1545	the expression of β-defensins	1532:1560	In addition, seasonal variations in the expression of β-defensins play a protective role in intestinal immunity.					
31708889	0	27	theme	Host	77:80	arg1	Feeding					82:88	Host Feeding	77:88	Host Feeding	77:88	Metagenomics Reveals Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator.					
31708889	5	28	theme	oligosaccharide-degrading	1073:1097	arg1	enzyme					1099:1104	mucin oligosaccharide-degrading enzyme	1067:1104	mucin oligosaccharide-degrading enzyme	1067:1104	This is accompanied by the enrichment of mucin oligosaccharide-degrading enzyme and carbohydrate-active enzyme families.					
31708889	2	29	theme	active	492:497	arg1	alligators					507:516	hibernating vs. active Chinese alligators	476:516	hibernating vs. active Chinese alligators	476:516	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	2	30	theme	hibernating	476:486	arg1	alligators					507:516	hibernating vs. active Chinese alligators	476:516	hibernating vs. active Chinese alligators	476:516	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	2	31	used	used	339:342	arg2	we					336:337	we	336:337	we	336:337	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	2	32	theme	rRNA	348:351	arg1	profiling					353:361	16S rRNA profiling	344:361	16S rRNA profiling	344:361	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	6	33	theme	known	1381:1385	arg1	functions					1428:1436	known proteolytic and amino acids-fermentating functions	1381:1436	known proteolytic and amino acids-fermentating functions	1381:1436	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	0	34	theme	Seasonal	21:28	arg1	Adaptation					41:50	Seasonal Functional Adaptation	21:50	Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator	21:125	Metagenomics Reveals Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator.					
31708889	1	35	theme	community	265:273	arg1	structure					275:283	microbial community structure	255:283	microbial community structure	255:283	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	3	36	theme	feeding	632:638	arg1	cycles					622:627	seasonal cycles	613:627	seasonal cycles of feeding and fasting in the Chinese alligator	613:675	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	6	37	dep	Cetobacterium	1353:1365	arg1	e.g.					1347:1350	e.g.	1347:1350	e.g.	1347:1350	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	6	37	dep	Cetobacterium	1353:1365	arg1	somerae					1367:1373	Cetobacterium somerae	1353:1373	Cetobacterium somerae	1353:1373	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	4	38	theme	host	963:966	arg1	glycans					974:980	host mucin glycans	963:980	host mucin glycans	963:980	During hibernation, there is an increase in the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans, which allows adaptation to winter fasting.					
31708889	6	39	theme	bacteria	1337:1344	arg1	abundance					1324:1332	the relative abundance	1311:1332	the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions	1311:1436	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	5	40	theme	enzyme	1130:1135	arg1	families					1137:1144	carbohydrate-active enzyme families	1110:1144	carbohydrate-active enzyme families	1110:1144	This is accompanied by the enrichment of mucin oligosaccharide-degrading enzyme and carbohydrate-active enzyme families.					
31708889	6	41	theme	host	1451:1454	arg1	efficiency					1484:1493	host protein-rich food digestion efficiency	1451:1493	host protein-rich food digestion efficiency	1451:1493	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	3	42	theme	fasting	644:650	arg1	cycles					622:627	seasonal cycles	613:627	seasonal cycles of feeding and fasting in the Chinese alligator	613:675	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	6	43	theme	acids-fermentating	1409:1426	arg1	functions					1428:1436	known proteolytic and amino acids-fermentating functions	1381:1436	known proteolytic and amino acids-fermentating functions	1381:1436	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	4	44	theme	winter	1010:1015	arg1	fasting					1017:1023	winter fasting	1010:1023	winter fasting	1010:1023	During hibernation, there is an increase in the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans, which allows adaptation to winter fasting.					
31708889	6	45	theme	carnivore	1231:1239	arg1	microbiome					1245:1254	a carnivore gut microbiome	1229:1254	a carnivore gut microbiome dominated by Fusobacteria	1229:1280	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	8	46	theme	microbe	1685:1691	arg1	shifts					1723:1728	host-gut microbe symbioses to seasonal dietary shifts	1676:1728	host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states	1676:1811	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	6	47	theme	Chinese	1202:1208	arg1	alligators					1210:1219	active Chinese alligators	1195:1219	active Chinese alligators	1195:1219	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	8	48	theme	dietary	1715:1721	arg1	shifts					1723:1728	host-gut microbe symbioses to seasonal dietary shifts	1676:1728	host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states	1676:1811	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	1	49	theme	Alligator	176:184	arg1	alligator					165:173	the Chinese alligator	153:173	the Chinese alligator (Alligator sinensis)	153:194	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	1	49	theme	Alligator	176:184	arg1	sinensis					186:193	Alligator sinensis	176:193	Alligator sinensis	176:193	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	0	50	from	Feeding	82:88	arg1	Alligator					117:125	the Chinese Alligator	105:125	the Chinese Alligator	105:125	Metagenomics Reveals Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator.					
31708889	3	51	theme	gut	709:711	arg1	community					723:731	a core gut microbial community	702:731	a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions	702:837	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	6	52	theme	food	1469:1472	arg1	efficiency					1484:1493	host protein-rich food digestion efficiency	1451:1493	host protein-rich food digestion efficiency	1451:1493	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	2	53	theme	Chinese	499:505	arg1	alligators					507:516	hibernating vs. active Chinese alligators	476:516	hibernating vs. active Chinese alligators	476:516	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	3	54	theme	microbial	545:553	arg1	communities					555:565	gut microbial communities	541:565	gut microbial communities	541:565	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	8	55	theme	gut	1742:1744	arg1	homeostasis					1746:1756	gut homeostasis	1742:1756	gut homeostasis	1742:1756	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	7	56	theme	protective	1569:1578	arg1	role					1580:1583	a protective role	1567:1583	a protective role	1567:1583	In addition, seasonal variations in the expression of β-defensins play a protective role in intestinal immunity.					
31708889	4	57	from	increase	872:879	arg1	abundance					888:896	the abundance	884:896	the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans	884:980	During hibernation, there is an increase in the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans, which allows adaptation to winter fasting.					
31708889	6	58	with	bacteria	1337:1344	arg1	functions					1428:1436	known proteolytic and amino acids-fermentating functions	1381:1436	known proteolytic and amino acids-fermentating functions	1381:1436	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	0	59	from	Fasting	94:100	arg1	Alligator					117:125	the Chinese Alligator	105:125	the Chinese Alligator	105:125	Metagenomics Reveals Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator.					
31708889	6	60	theme	active	1171:1176	arg1	feeding					1185:1191	feeding	1185:1191	feeding	1185:1191	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	6	60	theme	active	1171:1176	arg1	phase					1178:1182	the active phase	1167:1182	the active phase (feeding)	1167:1192	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	3	61	theme	gut	827:829	arg1	regions					831:837	the gut regions	823:837	the gut regions	823:837	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	2	62	theme	gut	458:460	arg1	microbiome					462:471	the gut microbiome	454:471	the gut microbiome of hibernating vs. active Chinese alligators	454:516	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	2	63	from	composition	403:413	arg1	microbiome					462:471	the gut microbiome	454:471	the gut microbiome of hibernating vs. active Chinese alligators	454:516	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	5	64	theme	mucin	1067:1071	arg1	enzyme					1099:1104	mucin oligosaccharide-degrading enzyme	1067:1104	mucin oligosaccharide-degrading enzyme	1067:1104	This is accompanied by the enrichment of mucin oligosaccharide-degrading enzyme and carbohydrate-active enzyme families.					
31708889	1	65	from	changes	244:250	arg1	function					289:296	function	289:296	function	289:296	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	1	65	from	changes	244:250	arg1	structure					275:283	microbial community structure	255:283	microbial community structure	255:283	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	2	66	from	diversity	416:424	arg1	microbiome					462:471	the gut microbiome	454:471	the gut microbiome of hibernating vs. active Chinese alligators	454:516	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	6	67	theme	relative	1315:1322	arg1	abundance					1324:1332	the relative abundance	1311:1332	the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions	1311:1436	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	5	68	theme	enzyme	1099:1104	arg1	enrichment					1053:1062	the enrichment	1049:1062	the enrichment of mucin oligosaccharide-degrading enzyme and carbohydrate-active enzyme families	1049:1144	This is accompanied by the enrichment of mucin oligosaccharide-degrading enzyme and carbohydrate-active enzyme families.					
31708889	8	69	theme	physiological	1792:1804	arg1	states					1806:1811	extreme physiological states	1784:1811	extreme physiological states	1784:1811	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	4	70	theme	taxa	911:914	arg1	abundance					888:896	the abundance	884:896	the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans	884:980	During hibernation, there is an increase in the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans, which allows adaptation to winter fasting.					
31708889	8	71	dep	shifts	1723:1728	arg1	to					1703:1704	to	1703:1704	to	1703:1704	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	8	71	dep	shifts	1723:1728	arg1	maintain					1733:1740	maintain	1733:1740	maintain	1733:1740	These findings provide insights into the functional adaptations of host-gut microbe symbioses to seasonal dietary shifts to maintain gut homeostasis and health, especially in extreme physiological states.					
31708889	6	72	from	increase	1299:1306	arg1	abundance					1324:1332	the relative abundance	1311:1332	the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions	1311:1436	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	2	73	from	capacity	442:449	arg1	microbiome					462:471	the gut microbiome	454:471	the gut microbiome of hibernating vs. active Chinese alligators	454:516	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	1	74	theme	ideal	202:206	arg1	alligator					165:173	the Chinese alligator	153:173	the Chinese alligator (Alligator sinensis)	153:194	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	1	74	theme	ideal	202:206	arg1	model					223:227	an ideal and intriguing model	199:227	an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation	199:318	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	6	75	theme	proteolytic	1387:1397	arg1	functions					1428:1436	known proteolytic and amino acids-fermentating functions	1381:1436	known proteolytic and amino acids-fermentating functions	1381:1436	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	4	76	theme	mucin	968:972	arg1	glycans					974:980	host mucin glycans	963:980	host mucin glycans	963:980	During hibernation, there is an increase in the abundance of bacterial taxa (e.g., the genus Bacteroides) that can degrade host mucin glycans, which allows adaptation to winter fasting.					
31708889	2	77	theme	16S	344:346	arg1	rRNA					348:351	16S rRNA	344:351	16S rRNA profiling	344:361	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	0	78	theme	Functional	30:39	arg1	Adaptation					41:50	Seasonal Functional Adaptation	21:50	Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator	21:125	Metagenomics Reveals Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator.					
31708889	1	79	theme	microbial	255:263	arg1	structure					275:283	microbial community structure	255:283	microbial community structure	255:283	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	7	80	from	variations	1518:1527	arg1	expression					1536:1545	the expression	1532:1545	the expression of β-defensins	1532:1560	In addition, seasonal variations in the expression of β-defensins play a protective role in intestinal immunity.					
31708889	7	81	theme	seasonal	1509:1516	arg1	variations					1518:1527	seasonal variations	1509:1527	seasonal variations in the expression of β-defensins	1509:1560	In addition, seasonal variations in the expression of β-defensins play a protective role in intestinal immunity.					
31708889	2	82	theme	functional	431:440	arg1	capacity					442:449	functional capacity	431:449	functional capacity	431:449	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	3	83	theme	seasonal	613:620	arg1	cycles					622:627	seasonal cycles	613:627	seasonal cycles of feeding and fasting in the Chinese alligator	613:675	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	2	84	theme	metagenomic	367:377	arg1	analysis					379:386	metagenomic analysis	367:386	metagenomic analysis	367:386	In this study, we used 16S rRNA profiling and metagenomic analysis to compare the composition, diversity, and functional capacity in the gut microbiome of hibernating vs. active Chinese alligators.					
31708889	0	85	theme	Gut	59:61	arg1	Microbiome					63:72	the Gut Microbiome	55:72	the Gut Microbiome	55:72	Metagenomics Reveals Seasonal Functional Adaptation of the Gut Microbiome to Host Feeding and Fasting in the Chinese Alligator.					
31708889	5	86	theme	carbohydrate-active	1110:1128	arg1	families					1137:1144	carbohydrate-active enzyme families	1110:1144	carbohydrate-active enzyme families	1110:1144	This is accompanied by the enrichment of mucin oligosaccharide-degrading enzyme and carbohydrate-active enzyme families.					
31708889	6	87	theme	protein-rich	1456:1467	arg1	efficiency					1484:1493	host protein-rich food digestion efficiency	1451:1493	host protein-rich food digestion efficiency	1451:1493	In contrast, during the active phase (feeding), active Chinese alligators exhibit a carnivore gut microbiome dominated by Fusobacteria, and there is an increase in the relative abundance of bacteria (e.g., Cetobacterium somerae) with known proteolytic and amino acids-fermentating functions that improve host protein-rich food digestion efficiency.					
31708889	5	88	theme	families	1137:1144	arg1	enrichment					1053:1062	the enrichment	1049:1062	the enrichment of mucin oligosaccharide-degrading enzyme and carbohydrate-active enzyme families	1049:1144	This is accompanied by the enrichment of mucin oligosaccharide-degrading enzyme and carbohydrate-active enzyme families.					
31708889	3	89	dep	cycles	622:627	arg1	response					601:608	response	601:608	response	601:608	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	3	90	from	fasting	644:650	arg1	alligator					667:675	the Chinese alligator	655:675	the Chinese alligator	655:675	Our results show that gut microbial communities undergo seasonal restructuring in response to seasonal cycles of feeding and fasting in the Chinese alligator, but this animal harbors a core gut microbial community primarily dominated by Proteobacteria, Fusobacteria, Bacteroidetes, and Firmicutes across the gut regions.					
31708889	1	91	theme	intriguing	212:221	arg1	alligator					165:173	the Chinese alligator	153:173	the Chinese alligator (Alligator sinensis)	153:194	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
31708889	1	91	theme	intriguing	212:221	arg1	model					223:227	an ideal and intriguing model	199:227	an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation	199:318	As a natural hibernator, the Chinese alligator (Alligator sinensis) is an ideal and intriguing model to investigate changes in microbial community structure and function caused by hibernation.					
29793133	8	0	from	Resistance	1555:1564	arg1	EIS					1575:1577	EIS	1575:1577	EIS as an analytical signal	1575:1601	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	12	1	theme	high	1984:1987	arg1	reproducibility					1989:2003	high reproducibility	1984:2003	high reproducibility	1984:2003	The GCE/rGO-MWCNT/CS/CQD showed sensitivity, high reproducibility, specificity and rapid response for lysozyme which can be used in biomedical fields.					
29793133	9	2	theme	EIS	1641:1643	arg1	techniques					1645:1654	EIS techniques	1641:1654	EIS techniques	1641:1654	Using the obtained data from DPV and EIS techniques, two calibration curves were drawn.					
29793133	2	3	theme	reduced	298:304	arg1	oxide					315:319	reduced graphene oxide	298:319	reduced graphene oxide (rGO)	298:325	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	2	3	theme	reduced	298:304	arg1	rGO					322:324	rGO	322:324	rGO	322:324	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	5	4	theme	considerable	848:859	arg1	enhancement					861:871	considerable enhancement	848:871	considerable enhancement of the electrochemical signal	848:901	In addition, this nanocomposite allows considerable enhancement of the electrochemical signal and contributes to improving sensitivity.					
29793133	4	5	theme	better	652:657	arg1	immobilization					659:672	better immobilization	652:672	better immobilization of aptamers due to the existence of many amino and carboxyl functional groups	652:750	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	1	6	theme	lysozyme	216:223	arg1	determination					199:211	the electrochemical determination	179:211	the electrochemical determination of lysozyme	179:223	An aptamer-based method is described for the electrochemical determination of lysozyme.					
29793133	9	7	theme	calibration	1661:1671	arg1	curves					1673:1678	two calibration curves	1657:1678	two calibration curves	1657:1678	Using the obtained data from DPV and EIS techniques, two calibration curves were drawn.					
29793133	10	8	theme	low	1743:1745	arg1	LODs					1747:1750	two very low LODs	1734:1750	two very low LODs	1734:1750	The anti-lysozyme aptasensor proposed has two very low LODs.					
29793133	8	9	from	lysozyme	1435:1442	arg1	interface					1461:1469	the electrode interface	1447:1469	the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal	1447:1601	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	9	10	from	techniques	1645:1654	arg1	data					1623:1626	the obtained data	1610:1626	the obtained data from DPV and EIS techniques	1610:1654	Using the obtained data from DPV and EIS techniques, two calibration curves were drawn.					
29793133	8	11	theme	electrode	1451:1459	arg1	interface					1461:1469	the electrode interface	1447:1469	the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal	1447:1601	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	6	12	theme	amino-linked	949:960	arg1	aptamers					971:978	The amino-linked lysozyme aptamers	945:978	The amino-linked lysozyme aptamers	945:978	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	2	13	mod	modified	256:263	arg1	electrode					242:250	A glassy carbon electrode	226:250	A glassy carbon electrode	226:250	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	2	13	mod	modified	256:263	arg3	nanocomposite					272:284	a nanocomposite	270:284	a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS	270:432	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	12	14	theme	biomedical	2071:2080	arg1	fields					2082:2087	biomedical fields	2071:2087	biomedical fields	2071:2087	The GCE/rGO-MWCNT/CS/CQD showed sensitivity, high reproducibility, specificity and rapid response for lysozyme which can be used in biomedical fields.					
29793133	8	15	theme	immobilized	1385:1395	arg1	aptamer					1397:1403	the immobilized aptamer	1381:1403	the immobilized aptamer	1381:1403	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	5	16	theme	improving	922:930	arg1	sensitivity					932:942	improving sensitivity	922:942	improving sensitivity	922:942	In addition, this nanocomposite allows considerable enhancement of the electrochemical signal and contributes to improving sensitivity.					
29793133	11	17	theme	EIS	1901:1903	arg1	curves					1917:1922	EIS calibration curves	1901:1922	EIS calibration curves	1901:1922	These measures are 3.7 and 1.9 fmol L-1 within the wide detection ranges of 20 fmol L-1 to 10 nmol L-1, and 10 fmol L-1 to 100 nmol L-1 for DPV and EIS calibration curves, respectively.					
29793133	0	18	theme	rGO-MWCNTs/Chitosan/carbon	68:93	arg1	dot					103:105	rGO-MWCNTs/Chitosan/carbon quantum dot	68:105	rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme	68:135	An ultrasensitive and selective electrochemical aptasensor based on rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme.					
29793133	6	19	link	amino-linked	949:960	arg1	aptamers					971:978	The amino-linked lysozyme aptamers	945:978	The amino-linked lysozyme aptamers	945:978	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	5	20	theme	electrochemical	880:894	arg1	signal					896:901	the electrochemical signal	876:901	the electrochemical signal	876:901	In addition, this nanocomposite allows considerable enhancement of the electrochemical signal and contributes to improving sensitivity.					
29793133	8	21	theme	peak	1507:1510	arg1	current					1512:1518	the DPV peak current	1499:1518	the DPV peak current	1499:1518	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	4	22	theme	groups	745:750	arg1	existence					697:705	the existence	693:705	the existence of many amino and carboxyl functional groups	693:750	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	8	23	from	decrease	1487:1494	arg1	Resistance					1555:1564	Charge Transfer Resistance	1539:1564	Charge Transfer Resistance (Rct) in EIS as an analytical signal	1539:1601	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	8	23	from	decrease	1487:1494	arg1	current					1512:1518	the DPV peak current	1499:1518	the DPV peak current	1499:1518	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	8	23	from	decrease	1487:1494	arg1	Rct					1567:1569	Rct	1567:1569	Rct	1567:1569	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	2	24	theme	carbon	401:406	arg1	CQD					421:423	CQD	421:423	CQD	421:423	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	2	24	theme	carbon	401:406	arg1	dot					416:418	a synthesized carbon quantum dot	387:418	a synthesized carbon quantum dot (CQD)	387:424	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	6	25	theme	amino	1092:1096	arg1	nanocomposite					1112:1124	the nanocomposite	1108:1124	the nanocomposite using glutaraldehyde (GLA) linker	1108:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	25	theme	amino	1092:1096	arg1	aptamer					1076:1082	the aptamer	1072:1082	the aptamer	1072:1082	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	25	theme	amino	1092:1096	arg1	groups					1098:1103	the amino groups	1088:1103	the amino groups of the nanocomposite using glutaraldehyde (GLA) linker	1088:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	4	26	theme	amino	715:719	arg1	existence					697:705	the existence	693:705	the existence of many amino and carboxyl functional groups	693:750	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	8	27	theme	Charge	1539:1544	arg1	Resistance					1555:1564	Charge Transfer Resistance	1539:1564	Charge Transfer Resistance (Rct) in EIS as an analytical signal	1539:1601	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	8	27	theme	Charge	1539:1544	arg1	Rct					1567:1569	Rct	1567:1569	Rct	1567:1569	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	4	28	theme	carboxyl	725:732	arg1	groups					745:750	many amino and carboxyl functional groups	710:750	groups	745:750	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	2	29	theme	quantum	408:414	arg1	CQD					421:423	CQD	421:423	CQD	421:423	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	2	29	theme	quantum	408:414	arg1	dot					416:418	a synthesized carbon quantum dot	387:418	a synthesized carbon quantum dot (CQD)	387:424	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	2	30	theme	synthesized	389:399	arg1	CQD					421:423	CQD	421:423	CQD	421:423	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	2	30	theme	synthesized	389:399	arg1	dot					416:418	a synthesized carbon quantum dot	387:418	a synthesized carbon quantum dot (CQD)	387:424	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	8	31	from	increase	1527:1534	arg1	Resistance					1555:1564	Charge Transfer Resistance	1539:1564	Charge Transfer Resistance (Rct) in EIS as an analytical signal	1539:1601	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	8	31	from	increase	1527:1534	arg1	current					1512:1518	the DPV peak current	1499:1518	the DPV peak current	1499:1518	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	8	31	from	increase	1527:1534	arg1	Rct					1567:1569	Rct	1567:1569	Rct	1567:1569	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	0	32	theme	electrochemical	32:46	arg1	aptasensor					48:57	An ultrasensitive and selective electrochemical aptasensor	0:57	An ultrasensitive and selective electrochemical aptasensor	0:57	An ultrasensitive and selective electrochemical aptasensor based on rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme.					
29793133	7	33	theme	electrochemical	1306:1320	arg1	EIS					1346:1348	EIS	1346:1348	EIS	1346:1348	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	7	33	theme	electrochemical	1306:1320	arg1	spectroscopy					1332:1343	electrochemical impedance spectroscopy	1306:1343	electrochemical impedance spectroscopy (EIS)	1306:1349	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	6	34	theme	GLA	1148:1150	arg1	linker					1153:1158	glutaraldehyde (GLA) linker	1132:1158	glutaraldehyde (GLA) linker	1132:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	3	35	theme	electrocatalytic	577:592	arg1	activity					594:601	great electrocatalytic activity	571:601	great electrocatalytic activity	571:601	The composition of the nanocomposite (rGO-MWCNT/CS/CQD) warrants a high surface-to-volume ratio, high conductivity, high stability, and great electrocatalytic activity.					
29793133	2	36	theme	glassy	228:233	arg1	electrode					242:250	A glassy carbon electrode	226:250	A glassy carbon electrode	226:250	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	4	37	theme	oxygen-related	767:780	arg1	defects					782:788	remaining oxygen-related defects	757:788	remaining oxygen-related defects	757:788	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	11	38	theme	detection	1809:1817	arg1	ranges					1819:1824	the wide detection ranges	1800:1824	the wide detection ranges of 20 fmol L-1 to 10 nmol L-1	1800:1854	These measures are 3.7 and 1.9 fmol L-1 within the wide detection ranges of 20 fmol L-1 to 10 nmol L-1, and 10 fmol L-1 to 100 nmol L-1 for DPV and EIS calibration curves, respectively.					
29793133	3	39	theme	high	502:505	arg1	ratio					525:529	a high surface-to-volume ratio	500:529	a high surface-to-volume ratio	500:529	The composition of the nanocomposite (rGO-MWCNT/CS/CQD) warrants a high surface-to-volume ratio, high conductivity, high stability, and great electrocatalytic activity.					
29793133	6	40	theme	glutaraldehyde	1132:1145	arg1	linker					1153:1158	glutaraldehyde (GLA) linker	1132:1158	glutaraldehyde (GLA) linker	1132:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	9	41	theme	obtained	1614:1621	arg1	data					1623:1626	the obtained data	1610:1626	the obtained data from DPV and EIS techniques	1610:1654	Using the obtained data from DPV and EIS techniques, two calibration curves were drawn.					
29793133	6	42	theme	nanocomposite	1112:1124	arg1	nanocomposite					1112:1124	the nanocomposite	1108:1124	the nanocomposite using glutaraldehyde (GLA) linker	1108:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	42	theme	nanocomposite	1112:1124	arg1	groups					1062:1067	the amino groups	1052:1067	the amino groups of the aptamer	1052:1082	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	42	theme	nanocomposite	1112:1124	arg1	aptamer					1076:1082	the aptamer	1072:1082	the aptamer	1072:1082	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	42	theme	nanocomposite	1112:1124	arg1	groups					1098:1103	the amino groups	1088:1103	the amino groups of the nanocomposite using glutaraldehyde (GLA) linker	1088:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	8	43	theme	analytical	1585:1594	arg1	signal					1596:1601	an analytical signal	1582:1601	an analytical signal	1582:1601	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	12	44	theme	rapid	2022:2026	arg1	response					2028:2035	rapid response	2022:2035	rapid response	2022:2035	The GCE/rGO-MWCNT/CS/CQD showed sensitivity, high reproducibility, specificity and rapid response for lysozyme which can be used in biomedical fields.					
29793133	3	45	theme	surface-to-volume	507:523	arg1	ratio					525:529	a high surface-to-volume ratio	500:529	a high surface-to-volume ratio	500:529	The composition of the nanocomposite (rGO-MWCNT/CS/CQD) warrants a high surface-to-volume ratio, high conductivity, high stability, and great electrocatalytic activity.					
29793133	2	46	theme	graphene	306:313	arg1	oxide					315:319	reduced graphene oxide	298:319	reduced graphene oxide (rGO)	298:325	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	2	46	theme	graphene	306:313	arg1	rGO					322:324	rGO	322:324	rGO	322:324	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	1	47	theme	electrochemical	183:197	arg1	determination					199:211	the electrochemical determination	179:211	the electrochemical determination of lysozyme	179:223	An aptamer-based method is described for the electrochemical determination of lysozyme.					
29793133	4	48	theme	suitable	634:641	arg1	site					643:646	a suitable site	632:646	a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups	632:750	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	2	49	theme	multi-walled	328:339	arg1	MWCNTs					359:364	MWCNTs	359:364	MWCNTs	359:364	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	2	49	theme	multi-walled	328:339	arg1	nanotubes					348:356	multi-walled carbon nanotubes	328:356	multi-walled carbon nanotubes (MWCNTs)	328:365	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	7	50	theme	modified	1165:1172	arg1	electrode					1174:1182	The modified electrode	1161:1182	The modified electrode	1161:1182	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	3	51	theme	nanocomposite	458:470	arg1	composition					439:449	The composition	435:449	The composition of the nanocomposite (rGO-MWCNT/CS/CQD)	435:489	The composition of the nanocomposite (rGO-MWCNT/CS/CQD) warrants a high surface-to-volume ratio, high conductivity, high stability, and great electrocatalytic activity.					
29793133	8	52	dep	lysozyme	1371:1378	arg1	presence					1359:1366	presence	1359:1366	presence	1359:1366	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	8	52	dep	lysozyme	1371:1378	arg1	the					1355:1357	the	1355:1357	the	1355:1357	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	9	53	from	DPV	1633:1635	arg1	data					1623:1626	the obtained data	1610:1626	the obtained data from DPV and EIS techniques	1610:1654	Using the obtained data from DPV and EIS techniques, two calibration curves were drawn.					
29793133	11	54	theme	wide	1804:1807	arg1	ranges					1819:1824	the wide detection ranges	1800:1824	the wide detection ranges of 20 fmol L-1 to 10 nmol L-1	1800:1854	These measures are 3.7 and 1.9 fmol L-1 within the wide detection ranges of 20 fmol L-1 to 10 nmol L-1, and 10 fmol L-1 to 100 nmol L-1 for DPV and EIS calibration curves, respectively.					
29793133	3	55	theme	high	551:554	arg1	stability					556:564	high stability	551:564	high stability	551:564	The composition of the nanocomposite (rGO-MWCNT/CS/CQD) warrants a high surface-to-volume ratio, high conductivity, high stability, and great electrocatalytic activity.					
29793133	12	56	used	used	2063:2066	arg2	sensitivity					1971:1981	sensitivity	1971:1981	sensitivity	1971:1981	The GCE/rGO-MWCNT/CS/CQD showed sensitivity, high reproducibility, specificity and rapid response for lysozyme which can be used in biomedical fields.					
29793133	12	56	used	used	2063:2066	arg2	response					2028:2035	rapid response	2022:2035	rapid response	2022:2035	The GCE/rGO-MWCNT/CS/CQD showed sensitivity, high reproducibility, specificity and rapid response for lysozyme which can be used in biomedical fields.					
29793133	12	56	used	used	2063:2066	arg2	specificity					2006:2016	specificity	2006:2016	specificity	2006:2016	The GCE/rGO-MWCNT/CS/CQD showed sensitivity, high reproducibility, specificity and rapid response for lysozyme which can be used in biomedical fields.					
29793133	12	56	used	used	2063:2066	arg2	reproducibility					1989:2003	high reproducibility	1984:2003	high reproducibility	1984:2003	The GCE/rGO-MWCNT/CS/CQD showed sensitivity, high reproducibility, specificity and rapid response for lysozyme which can be used in biomedical fields.					
29793133	1	57	theme	aptamer-based	141:153	arg1	method					155:160	An aptamer-based method	138:160	An aptamer-based method	138:160	An aptamer-based method is described for the electrochemical determination of lysozyme.					
29793133	6	58	theme	amino	1056:1060	arg1	nanocomposite					1112:1124	the nanocomposite	1108:1124	the nanocomposite using glutaraldehyde (GLA) linker	1108:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	58	theme	amino	1056:1060	arg1	groups					1062:1067	the amino groups	1052:1067	the amino groups of the aptamer	1052:1082	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	58	theme	amino	1056:1060	arg1	aptamer					1076:1082	the aptamer	1072:1082	the aptamer	1072:1082	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	0	59	theme	quantum	95:101	arg1	dot					103:105	rGO-MWCNTs/Chitosan/carbon quantum dot	68:105	rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme	68:135	An ultrasensitive and selective electrochemical aptasensor based on rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme.					
29793133	3	60	theme	great	571:575	arg1	activity					594:601	great electrocatalytic activity	571:601	great electrocatalytic activity	571:601	The composition of the nanocomposite (rGO-MWCNT/CS/CQD) warrants a high surface-to-volume ratio, high conductivity, high stability, and great electrocatalytic activity.					
29793133	10	61	contain	has	1730:1732	arg2	LODs					1747:1750	two very low LODs	1734:1750	two very low LODs	1734:1750	The anti-lysozyme aptasensor proposed has two very low LODs.					
29793133	10	61	contain	has	1730:1732	arg1	aptasensor					1710:1719	The anti-lysozyme aptasensor	1692:1719	The anti-lysozyme aptasensor proposed	1692:1728	The anti-lysozyme aptasensor proposed has two very low LODs.					
29793133	8	62	theme	DPV	1503:1505	arg1	current					1512:1518	the DPV peak current	1499:1518	the DPV peak current	1499:1518	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	7	63	theme	differential	1239:1250	arg1	voltammetry					1258:1268	differential pulse voltammetry	1239:1268	differential pulse voltammetry (DPV)	1239:1274	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	7	63	theme	differential	1239:1250	arg1	DPV					1271:1273	DPV	1271:1273	DPV	1271:1273	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	7	64	theme	pulse	1252:1256	arg1	voltammetry					1258:1268	differential pulse voltammetry	1239:1268	differential pulse voltammetry (DPV)	1239:1274	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	7	64	theme	pulse	1252:1256	arg1	DPV					1271:1273	DPV	1271:1273	DPV	1271:1273	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	5	65	theme	signal	896:901	arg1	enhancement					861:871	considerable enhancement	848:871	considerable enhancement of the electrochemical signal	848:901	In addition, this nanocomposite allows considerable enhancement of the electrochemical signal and contributes to improving sensitivity.					
29793133	6	66	theme	covalent	1026:1033	arg1	coupling					1035:1042	covalent coupling	1026:1042	covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker	1026:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	67	theme	aptamer	1076:1082	arg1	nanocomposite					1112:1124	the nanocomposite	1108:1124	the nanocomposite using glutaraldehyde (GLA) linker	1108:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	67	theme	aptamer	1076:1082	arg1	groups					1062:1067	the amino groups	1052:1067	the amino groups of the aptamer	1052:1082	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	67	theme	aptamer	1076:1082	arg1	aptamer					1076:1082	the aptamer	1072:1082	the aptamer	1072:1082	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	6	67	theme	aptamer	1076:1082	arg1	groups					1098:1103	the amino groups	1088:1103	the amino groups of the nanocomposite using glutaraldehyde (GLA) linker	1088:1158	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	4	68	theme	aptamers	677:684	arg1	immobilization					659:672	better immobilization	652:672	better immobilization of aptamers due to the existence of many amino and carboxyl functional groups	652:750	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	8	69	theme	target	1428:1433	arg1	lysozyme					1435:1442	the target lysozyme	1424:1442	the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal	1424:1601	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	3	70	theme	high	532:535	arg1	conductivity					537:548	high conductivity	532:548	high conductivity	532:548	The composition of the nanocomposite (rGO-MWCNT/CS/CQD) warrants a high surface-to-volume ratio, high conductivity, high stability, and great electrocatalytic activity.					
29793133	4	71	theme	functional	734:743	arg1	groups					745:750	many amino and carboxyl functional groups	710:750	groups	745:750	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	0	72	theme	lysozyme	128:135	arg1	detection					115:123	the detection	111:123	the detection of lysozyme	111:135	An ultrasensitive and selective electrochemical aptasensor based on rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme.					
29793133	7	73	theme	electrochemical	1205:1219	arg1	spectroscopy					1332:1343	electrochemical impedance spectroscopy	1306:1343	electrochemical impedance spectroscopy (EIS)	1306:1349	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	7	73	theme	electrochemical	1205:1219	arg1	voltammetry					1258:1268	differential pulse voltammetry	1239:1268	differential pulse voltammetry (DPV)	1239:1274	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	7	73	theme	electrochemical	1205:1219	arg1	voltammetry					1284:1294	cyclic voltammetry	1277:1294	cyclic voltammetry (CV)	1277:1299	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	7	73	theme	electrochemical	1205:1219	arg1	methods					1221:1227	electrochemical methods	1205:1227	electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS)	1205:1349	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	0	74	theme	ultrasensitive	3:16	arg1	aptasensor					48:57	An ultrasensitive and selective electrochemical aptasensor	0:57	An ultrasensitive and selective electrochemical aptasensor	0:57	An ultrasensitive and selective electrochemical aptasensor based on rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme.					
29793133	8	75	theme	Transfer	1546:1553	arg1	Resistance					1555:1564	Charge Transfer Resistance	1539:1564	Charge Transfer Resistance (Rct) in EIS as an analytical signal	1539:1601	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	8	75	theme	Transfer	1546:1553	arg1	Rct					1567:1569	Rct	1567:1569	Rct	1567:1569	In the presence of lysozyme, the immobilized aptamer selectively caught the target lysozyme on the electrode interface that leads to a decrease in the DPV peak current and an increase in Charge Transfer Resistance (Rct) in EIS as an analytical signal.					
29793133	4	76	theme	many	710:713	arg1	amino					715:719	many amino and carboxyl functional groups	710:750	amino	715:719	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	0	77	theme	selective	22:30	arg1	aptasensor					48:57	An ultrasensitive and selective electrochemical aptasensor	0:57	An ultrasensitive and selective electrochemical aptasensor	0:57	An ultrasensitive and selective electrochemical aptasensor based on rGO-MWCNTs/Chitosan/carbon quantum dot for the detection of lysozyme.					
29793133	7	78	theme	impedance	1322:1330	arg1	EIS					1346:1348	EIS	1346:1348	EIS	1346:1348	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	7	78	theme	impedance	1322:1330	arg1	spectroscopy					1332:1343	electrochemical impedance spectroscopy	1306:1343	electrochemical impedance spectroscopy (EIS)	1306:1349	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	11	79	theme	calibration	1905:1915	arg1	curves					1917:1922	EIS calibration curves	1901:1922	EIS calibration curves	1901:1922	These measures are 3.7 and 1.9 fmol L-1 within the wide detection ranges of 20 fmol L-1 to 10 nmol L-1, and 10 fmol L-1 to 100 nmol L-1 for DPV and EIS calibration curves, respectively.					
29793133	6	80	theme	lysozyme	962:969	arg1	aptamers					971:978	The amino-linked lysozyme aptamers	945:978	The amino-linked lysozyme aptamers	945:978	The amino-linked lysozyme aptamers were immobilized on the nanocomposite through covalent coupling between the amino groups of the aptamer and the amino groups of the nanocomposite using glutaraldehyde (GLA) linker.					
29793133	11	81	dep	100 nmol L-1	1876:1887	arg1	to					1873:1874	to	1873:1874	to	1873:1874	These measures are 3.7 and 1.9 fmol L-1 within the wide detection ranges of 20 fmol L-1 to 10 nmol L-1, and 10 fmol L-1 to 100 nmol L-1 for DPV and EIS calibration curves, respectively.					
29793133	2	82	theme	carbon	235:240	arg1	electrode					242:250	A glassy carbon electrode	226:250	A glassy carbon electrode	226:250	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	11	83	theme	20 fmol L-1	1829:1839	arg1	ranges					1819:1824	the wide detection ranges	1800:1824	the wide detection ranges of 20 fmol L-1 to 10 nmol L-1	1800:1854	These measures are 3.7 and 1.9 fmol L-1 within the wide detection ranges of 20 fmol L-1 to 10 nmol L-1, and 10 fmol L-1 to 100 nmol L-1 for DPV and EIS calibration curves, respectively.					
29793133	4	84	theme	remaining	757:765	arg1	defects					782:788	remaining oxygen-related defects	757:788	remaining oxygen-related defects	757:788	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	2	85	theme	carbon	341:346	arg1	MWCNTs					359:364	MWCNTs	359:364	MWCNTs	359:364	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	2	85	theme	carbon	341:346	arg1	nanotubes					348:356	multi-walled carbon nanotubes	328:356	multi-walled carbon nanotubes (MWCNTs)	328:365	A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS.					
29793133	4	86	dep	site	643:646	arg1	properties					790:799	properties	790:799	properties	790:799	This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO.					
29793133	7	87	theme	cyclic	1277:1282	arg1	CV					1297:1298	CV	1297:1298	CV	1297:1298	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	7	87	theme	cyclic	1277:1282	arg1	voltammetry					1284:1294	cyclic voltammetry	1277:1294	cyclic voltammetry (CV)	1277:1299	The modified electrode was characterized by electrochemical methods including differential pulse voltammetry (DPV), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS).					
29793133	10	88	theme	anti-lysozyme	1696:1708	arg1	aptasensor					1710:1719	The anti-lysozyme aptasensor	1692:1719	The anti-lysozyme aptasensor proposed	1692:1728	The anti-lysozyme aptasensor proposed has two very low LODs.					
29767309	5	0	theme	dried	721:725	arg1	CMC-Fe/Ni					727:735	the dried CMC-Fe/Ni	717:735	the dried CMC-Fe/Ni	717:735	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	4	1	theme	background	478:487	arg1	solution					489:496	background solution	478:496	background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds)	478:647	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	8	2	contain	had	1403:1405	arg1	addition					1345:1352	the individual addition	1330:1352	the individual addition of external solutes into background solution	1330:1397	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	8	2	contain	had	1403:1405	arg2	effect					1418:1423	an adverse effect	1407:1423	an adverse effect	1407:1423	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	4	3	theme	precursors	564:573	arg1	preparation					536:546	the preparation	532:546	the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds)	532:647	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	6	4	theme	experimental	862:873	arg1	results					875:881	The experimental results	858:881	The experimental results	858:881	The experimental results indicated that CMC stabilization greatly improved the reactivity of Fe/Ni bimetal and CF (10 mg/L) could be completely degraded by CMC-Fe/Ni (0.1 g/L) within 45 min.					
29767309	8	5	theme	CMC-Fe/Ni	1446:1454	arg1	significant					1519:1529	significant	1519:1529	significant	1519:1529	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	8	5	theme	CMC-Fe/Ni	1446:1454	arg1	reactivity					1432:1441	the reactivity	1428:1441	the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite	1428:1558	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	8	5	theme	CMC-Fe/Ni	1446:1454	arg1	effect					1480:1485	the poisoning effect	1466:1485	the poisoning effect of sulfides (Na2S, Na2S2O4)	1466:1513	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	7	6	theme	different	1060:1068	arg1	precursors					1076:1085	different Fe/Ni precursors	1060:1085	different Fe/Ni precursors resulting in the variations of background solution	1060:1136	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	4	7	theme	Fe/Ni	558:562	arg1	precursors					564:573	Fe/Ni (Fe/Ni precursors	551:573	Fe/Ni (Fe/Ni precursors	551:573	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	7	8	theme	negative	1266:1273	arg1	correlation					1275:1285	a negative correlation	1264:1285	a negative correlation with the reactivity of CMC-Fe/Ni	1264:1318	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	7	9	theme	background	1237:1246	arg1	solution					1248:1255	background solution	1237:1255	background solution	1237:1255	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	4	10	theme	NaBH4	576:580	arg1	dosage					582:587	NaBH4 dosage)	576:588	NaBH4 dosage)	576:588	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	7	11	from	dosage	1218:1223	arg1	solution					1248:1255	background solution	1237:1255	background solution	1237:1255	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	4	12	theme	CMC-Fe/Ni	670:678	arg1	reactivity					656:665	the reactivity	652:665	the reactivity of CMC-Fe/Ni	652:678	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	7	13	theme	solution	1129:1136	arg1	variations					1104:1113	the variations	1100:1113	the variations of background solution	1100:1136	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	8	14	theme	individual	1334:1343	arg1	addition					1345:1352	the individual addition	1330:1352	the individual addition of external solutes into background solution	1330:1397	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	5	15	theme	microscopy	797:806	arg1	terms					770:774	terms	770:774	terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS)	770:855	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	4	16	theme	addition	598:605	arg1	preparation					536:546	the preparation	532:546	the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds)	532:647	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	5	17	theme	X-ray	818:822	arg1	XPS					852:854	XPS	852:854	XPS	852:854	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	5	17	theme	X-ray	818:822	arg1	spectroscopy					838:849	X-ray photoelectron spectroscopy	818:849	X-ray photoelectron spectroscopy (XPS)	818:855	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	1	18	theme	nanoparticles	158:170	arg1	loss					150:153	the reactivity loss	135:153	the reactivity loss of nanoparticles due to aggregation	135:189	The use of stabilizers can prevent the reactivity loss of nanoparticles due to aggregation.					
29767309	4	19	from	effect	468:473	arg1	reactivity					656:665	the reactivity	652:665	the reactivity of CMC-Fe/Ni	652:678	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	3	20	theme	chloroform	435:444	arg1	degradation					451:461	chloroform (CF) degradation	435:461	chloroform (CF) degradation	435:461	The reactivity of CMC-Fe/Ni was evaluated via the reaction of chloroform (CF) degradation.					
29767309	7	21	theme	obvious	1156:1162	arg1	influence					1164:1172	no obvious influence	1153:1172	no obvious influence	1153:1172	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	2	22	theme	CMC-stabilized	297:310	arg1	CMC-Fe/Ni					327:335	CMC-Fe/Ni	327:335	CMC-Fe/Ni	327:335	In this study, carboxymethylcellulose (CMC) was selected as the stabilizer to synthesize a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni) via pre-aggregation stabilization.					
29767309	2	22	theme	CMC-stabilized	297:310	arg1	colloid					318:324	a highly stable CMC-stabilized Fe/Ni colloid	281:324	a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni)	281:336	In this study, carboxymethylcellulose (CMC) was selected as the stabilizer to synthesize a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni) via pre-aggregation stabilization.					
29767309	4	23	theme	sulfur	631:636	arg1	ions					625:628	common ions	618:628	common ions	618:628	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	4	23	theme	sulfur	631:636	arg1	compounds					638:646	sulfur compounds	631:646	sulfur compounds	631:646	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	7	24	theme	background	1118:1127	arg1	solution					1129:1136	background solution	1118:1136	background solution	1118:1136	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	2	25	theme	stable	290:295	arg1	CMC-Fe/Ni					327:335	CMC-Fe/Ni	327:335	CMC-Fe/Ni	327:335	In this study, carboxymethylcellulose (CMC) was selected as the stabilizer to synthesize a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni) via pre-aggregation stabilization.					
29767309	2	25	theme	stable	290:295	arg1	colloid					318:324	a highly stable CMC-stabilized Fe/Ni colloid	281:324	a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni)	281:336	In this study, carboxymethylcellulose (CMC) was selected as the stabilizer to synthesize a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni) via pre-aggregation stabilization.					
29767309	5	26	theme	photoelectron	824:836	arg1	XPS					852:854	XPS	852:854	XPS	852:854	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	5	26	theme	photoelectron	824:836	arg1	spectroscopy					838:849	X-ray photoelectron spectroscopy	818:849	X-ray photoelectron spectroscopy (XPS)	818:855	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	0	27	theme	Fe/Ni	18:22	arg1	colloid					24:30	Fe/Ni colloid	18:30	Fe/Ni colloid	18:30	The reactivity of Fe/Ni colloid stabilized by carboxymethylcellulose (CMC-Fe/Ni) toward chloroform.					
29767309	8	28	theme	poisoning	1470:1478	arg1	significant					1519:1529	significant	1519:1529	significant	1519:1529	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	8	28	theme	poisoning	1470:1478	arg1	effect					1480:1485	the poisoning effect	1466:1485	the poisoning effect of sulfides (Na2S, Na2S2O4)	1466:1513	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	7	29	theme	CMC-Fe/Ni	1310:1318	arg1	reactivity					1296:1305	the reactivity	1292:1305	the reactivity of CMC-Fe/Ni	1292:1318	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	5	30	theme	electron	788:795	arg1	SEM					809:811	SEM	809:811	SEM	809:811	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	5	30	theme	electron	788:795	arg1	microscopy					797:806	scanning electron microscopy	779:806	scanning electron microscopy (SEM)	779:812	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	5	31	theme	scanning	779:786	arg1	SEM					809:811	SEM	809:811	SEM	809:811	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	5	31	theme	scanning	779:786	arg1	microscopy					797:806	scanning electron microscopy	779:806	scanning electron microscopy (SEM)	779:812	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	8	32	theme	sulfides	1490:1497	arg1	significant					1519:1529	significant	1519:1529	significant	1519:1529	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	8	32	theme	sulfides	1490:1497	arg1	effect					1480:1485	the poisoning effect	1466:1485	the poisoning effect of sulfides (Na2S, Na2S2O4)	1466:1513	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	3	33	theme	degradation	451:461	arg1	reaction					423:430	the reaction	419:430	the reaction of chloroform (CF) degradation	419:461	The reactivity of CMC-Fe/Ni was evaluated via the reaction of chloroform (CF) degradation.					
29767309	7	34	theme	NaBH4	1228:1232	arg1	dosage					1218:1223	the dosage	1214:1223	the dosage of NaBH4 in background solution	1214:1255	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	5	35	used	used	741:744	arg2	CMC-Fe/Ni					727:735	the dried CMC-Fe/Ni	717:735	the dried CMC-Fe/Ni	717:735	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	8	36	theme	background	1379:1388	arg1	solution					1390:1397	background solution	1379:1397	background solution	1379:1397	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	7	37	theme	Fe/Ni	1070:1074	arg1	precursors					1076:1085	different Fe/Ni precursors	1060:1085	different Fe/Ni precursors resulting in the variations of background solution	1060:1136	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	0	38	theme	colloid	24:30	arg1	reactivity					4:13	The reactivity	0:13	The reactivity of Fe/Ni colloid	0:30	The reactivity of Fe/Ni colloid stabilized by carboxymethylcellulose (CMC-Fe/Ni) toward chloroform.					
29767309	6	39	theme	bimetal	957:963	arg1	reactivity					937:946	the reactivity	933:946	the reactivity of Fe/Ni bimetal	933:963	The experimental results indicated that CMC stabilization greatly improved the reactivity of Fe/Ni bimetal and CF (10 mg/L) could be completely degraded by CMC-Fe/Ni (0.1 g/L) within 45 min.					
29767309	4	40	dep	solute	610:615	arg1	ions					625:628	common ions	618:628	common ions	618:628	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	4	40	dep	solute	610:615	arg1	compounds					638:646	sulfur compounds	631:646	sulfur compounds	631:646	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	4	41	theme	solute	610:615	arg1	addition					598:605	the addition	594:605	the addition of solute (common ions, sulfur compounds)	594:647	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	4	41	theme	solute	610:615	arg1	precursors					564:573	Fe/Ni (Fe/Ni precursors	551:573	Fe/Ni (Fe/Ni precursors	551:573	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	4	41	theme	solute	610:615	arg1	dosage					582:587	NaBH4 dosage)	576:588	NaBH4 dosage)	576:588	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	3	42	theme	CMC-Fe/Ni	391:399	arg1	reactivity					377:386	The reactivity	373:386	The reactivity of CMC-Fe/Ni	373:399	The reactivity of CMC-Fe/Ni was evaluated via the reaction of chloroform (CF) degradation.					
29767309	6	43	theme	CMC	898:900	arg1	stabilization					902:914	CMC stabilization	898:914	CMC stabilization	898:914	The experimental results indicated that CMC stabilization greatly improved the reactivity of Fe/Ni bimetal and CF (10 mg/L) could be completely degraded by CMC-Fe/Ni (0.1 g/L) within 45 min.					
29767309	7	44	theme	precursors	1076:1085	arg1	use					1053:1055	The use	1049:1055	The use of different Fe/Ni precursors resulting in the variations of background solution	1049:1136	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	7	45	dep	seemed	1138:1143	arg1	whereas					1206:1212	whereas	1206:1212	whereas	1206:1212	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	8	46	theme	adverse	1410:1416	arg1	effect					1418:1423	an adverse effect	1407:1423	an adverse effect	1407:1423	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	1	47	theme	reactivity	139:148	arg1	loss					150:153	the reactivity loss	135:153	the reactivity loss of nanoparticles due to aggregation	135:189	The use of stabilizers can prevent the reactivity loss of nanoparticles due to aggregation.					
29767309	7	48	contain	have	1148:1151	arg1	use					1053:1055	The use	1049:1055	The use of different Fe/Ni precursors resulting in the variations of background solution	1049:1136	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	7	48	contain	have	1148:1151	arg2	influence					1164:1172	no obvious influence	1153:1172	no obvious influence	1153:1172	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	1	49	theme	stabilizers	111:121	arg1	use					104:106	The use	100:106	The use of stabilizers	100:121	The use of stabilizers can prevent the reactivity loss of nanoparticles due to aggregation.					
29767309	7	50	theme	CMC-Fe/Ni	1195:1203	arg1	reactivity					1181:1190	the reactivity	1177:1190	the reactivity of CMC-Fe/Ni	1177:1203	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	8	51	theme	common	1536:1541	arg1	ions					1543:1546	common ions	1536:1546	common ions	1536:1546	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	8	52	theme	external	1357:1364	arg1	solutes					1366:1372	external solutes	1357:1372	external solutes	1357:1372	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	2	53	theme	Fe/Ni	312:316	arg1	CMC-Fe/Ni					327:335	CMC-Fe/Ni	327:335	CMC-Fe/Ni	327:335	In this study, carboxymethylcellulose (CMC) was selected as the stabilizer to synthesize a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni) via pre-aggregation stabilization.					
29767309	2	53	theme	Fe/Ni	312:316	arg1	colloid					318:324	a highly stable CMC-stabilized Fe/Ni colloid	281:324	a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni)	281:336	In this study, carboxymethylcellulose (CMC) was selected as the stabilizer to synthesize a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni) via pre-aggregation stabilization.					
29767309	4	54	theme	dosage	582:587	arg1	preparation					536:546	the preparation	532:546	the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds)	532:647	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	7	55	with	correlation	1275:1285	arg1	reactivity					1296:1305	the reactivity	1292:1305	the reactivity of CMC-Fe/Ni	1292:1318	The use of different Fe/Ni precursors resulting in the variations of background solution seemed to have no obvious influence on the reactivity of CMC-Fe/Ni, whereas the dosage of NaBH4 in background solution showed a negative correlation with the reactivity of CMC-Fe/Ni.					
29767309	3	56	theme	CF	447:448	arg1	degradation					451:461	chloroform (CF) degradation	435:461	chloroform (CF) degradation	435:461	The reactivity of CMC-Fe/Ni was evaluated via the reaction of chloroform (CF) degradation.					
29767309	5	57	theme	spectroscopy	838:849	arg1	terms					770:774	terms	770:774	terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS)	770:855	Additionally, the dried CMC-Fe/Ni was used for characterization in terms of scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS).					
29767309	4	58	theme	common	618:623	arg1	ions					625:628	common ions	618:628	common ions	618:628	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	4	58	theme	common	618:623	arg1	compounds					638:646	sulfur compounds	631:646	sulfur compounds	631:646	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	6	59	theme	Fe/Ni	951:955	arg1	bimetal					957:963	Fe/Ni bimetal	951:963	Fe/Ni bimetal	951:963	The experimental results indicated that CMC stabilization greatly improved the reactivity of Fe/Ni bimetal and CF (10 mg/L) could be completely degraded by CMC-Fe/Ni (0.1 g/L) within 45 min.					
29767309	4	60	theme	solution	489:496	arg1	effect					468:473	The effect	464:473	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni	464:678	The effect of background solution which composition was affected by the preparation of Fe/Ni (Fe/Ni precursors, NaBH4 dosage) and the addition of solute (common ions, sulfur compounds) on the reactivity of CMC-Fe/Ni was also investigated.					
29767309	8	61	theme	solutes	1366:1372	arg1	addition					1345:1352	the individual addition	1330:1352	the individual addition of external solutes into background solution	1330:1397	Besides, the individual addition of external solutes into background solution all had an adverse effect on the reactivity of CMC-Fe/Ni, of which the poisoning effect of sulfides (Na2S, Na2S2O4) was significant than common ions and sulfite.					
29767309	2	62	theme	pre-aggregation	342:356	arg1	stabilization					358:370	pre-aggregation stabilization	342:370	pre-aggregation stabilization	342:370	In this study, carboxymethylcellulose (CMC) was selected as the stabilizer to synthesize a highly stable CMC-stabilized Fe/Ni colloid (CMC-Fe/Ni) via pre-aggregation stabilization.					
31013774	9	0	theme	thermal	941:947	arg1	conductivity					949:960	a low thermal conductivity	935:960	a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry)	935:998	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	1	from	C	992:992	arg1	K					981:981	m · K	977:981	m · K	977:981	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	1	from	C	992:992	arg1	mW/					973:975	67.9-69.0 mW/	963:975	67.9-69.0 mW/(m · K) at 23 ° C	963:992	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	1	from	C	992:992	arg1	dry					995:997	dry	995:997	dry	995:997	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	1	2	dep	meet	86:89	arg1	to					83:84	to	83:84	to	83:84	In order to meet the requirement of sustainable development, building materials are increasingly environmentally friendly.					
31013774	4	3	theme	wheat	510:514	arg1	straw					516:520	wheat straw	510:520	wheat straw	510:520	In a previous work, a feasibility study simultaneously investigated the processing and ratio of wheat straw required to ensure a gluing effect.					
31013774	8	4	theme	mechanical	821:830	arg1	properties					832:841	sufficiently high mechanical properties	803:841	sufficiently high mechanical properties	803:841	They showed sufficiently high mechanical properties to be used as insulating materials.					
31013774	5	5	theme	hemp-polysaccharides	643:662	arg1	composite					664:672	a hemp-polysaccharides composite	641:672	a hemp-polysaccharides composite	641:672	In this paper, three kinds of hemp-straw composites are selected and compared with a hemp-polysaccharides composite.					
31013774	8	6	theme	high	816:819	arg1	properties					832:841	sufficiently high mechanical properties	803:841	sufficiently high mechanical properties	803:841	They showed sufficiently high mechanical properties to be used as insulating materials.					
31013774	5	7	theme	composites	599:608	arg1	kinds					579:583	three kinds	573:583	three kinds of hemp-straw composites	573:608	In this paper, three kinds of hemp-straw composites are selected and compared with a hemp-polysaccharides composite.					
31013774	5	8	theme	hemp-straw	588:597	arg1	composites					599:608	hemp-straw composites	588:608	hemp-straw composites	588:608	In this paper, three kinds of hemp-straw composites are selected and compared with a hemp-polysaccharides composite.					
31013774	9	9	with	performances	917:928	arg1	conductivity					949:960	a low thermal conductivity	935:960	a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry)	935:998	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	10	theme	low	937:939	arg1	conductivity					949:960	a low thermal conductivity	935:960	a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry)	935:998	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	11	theme	·	979:979	arg1	K					981:981	m · K	977:981	m · K	977:981	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	11	theme	·	979:979	arg1	mW/					973:975	67.9-69.0 mW/	963:975	67.9-69.0 mW/(m · K) at 23 ° C	963:992	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	3	12	theme	binding	387:393	arg1	wheat					406:410	wheat	406:410	wheat	406:410	This paper looks at the development of fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat).					
31013774	3	12	theme	binding	387:393	arg1	materials					395:403	binding materials	387:403	binding materials (wheat)	387:411	This paper looks at the development of fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat).					
31013774	3	12	theme	binding	387:393	arg1	agro-resources					323:336	agro-resources	323:336	agro-resources	323:336	This paper looks at the development of fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat).					
31013774	4	13	theme	gluing	543:548	arg1	effect					550:555	a gluing effect	541:555	a gluing effect	541:555	In a previous work, a feasibility study simultaneously investigated the processing and ratio of wheat straw required to ensure a gluing effect.					
31013774	0	14	theme	Hemp-Straw	0:9	arg1	Composites					11:20	Hemp-Straw Composites	0:20	Hemp-Straw Composites: Gluing Study and Multi-Physical Characterizations.	0:72	Hemp-Straw Composites: Gluing Study and Multi-Physical Characterizations.					
31013774	4	15	dep	processing	486:495	arg1	the					482:484	the	482:484	the	482:484	In a previous work, a feasibility study simultaneously investigated the processing and ratio of wheat straw required to ensure a gluing effect.					
31013774	6	16	theme	gluing	679:684	arg1	effect					686:691	The gluing effect	675:691	The gluing effect	675:691	The gluing effect is analyzed chemically and via SEM.					
31013774	0	17	theme	Gluing	23:28	arg1	Study					30:34	Gluing Study	23:34	Gluing Study	23:34	Hemp-Straw Composites: Gluing Study and Multi-Physical Characterizations.					
31013774	9	18	dep	conductivity	949:960	arg1	K					981:981	m · K	977:981	m · K	977:981	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	18	dep	conductivity	949:960	arg1	mW/					973:975	67.9-69.0 mW/	963:975	67.9-69.0 mW/(m · K) at 23 ° C	963:992	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	18	dep	conductivity	949:960	arg1	dry					995:997	dry	995:997	dry	995:997	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	19	theme	°	990:990	arg1	C					992:992	23 ° C	987:992	23 ° C	987:992	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	4	20	theme	straw	516:520	arg1	ratio					501:505	ratio	501:505	ratio	501:505	In a previous work, a feasibility study simultaneously investigated the processing and ratio of wheat straw required to ensure a gluing effect.					
31013774	4	20	theme	straw	516:520	arg1	processing					486:495	processing	486:495	processing	486:495	In a previous work, a feasibility study simultaneously investigated the processing and ratio of wheat straw required to ensure a gluing effect.					
31013774	9	21	theme	good	904:907	arg1	performances					917:928	good thermal performances	904:928	good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry)	904:998	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	4	22	theme	feasibility	436:446	arg1	study					448:452	a feasibility study	434:452	a feasibility study	434:452	In a previous work, a feasibility study simultaneously investigated the processing and ratio of wheat straw required to ensure a gluing effect.					
31013774	1	23	theme	sustainable	110:120	arg1	development					122:132	sustainable development	110:132	sustainable development	110:132	In order to meet the requirement of sustainable development, building materials are increasingly environmentally friendly.					
31013774	0	24	theme	Multi-Physical	40:53	arg1	Characterizations					55:71	Multi-Physical Characterizations	40:71	Multi-Physical Characterizations	40:71	Hemp-Straw Composites: Gluing Study and Multi-Physical Characterizations.					
31013774	9	25	theme	thermal	909:915	arg1	performances					917:928	good thermal performances	904:928	good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry)	904:998	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	1	26	theme	development	122:132	arg1	requirement					95:105	the requirement	91:105	the requirement of sustainable development	91:132	In order to meet the requirement of sustainable development, building materials are increasingly environmentally friendly.					
31013774	3	27	theme	bio-based	296:304	arg1	composites					306:315	fully bio-based composites	290:315	fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat)	290:411	This paper looks at the development of fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat).					
31013774	3	28	theme	composites	306:315	arg1	development					275:285	the development	271:285	the development of fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat)	271:411	This paper looks at the development of fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat).					
31013774	1	29	theme	building	135:142	arg1	friendly					187:194	friendly	187:194	friendly	187:194	In order to meet the requirement of sustainable development, building materials are increasingly environmentally friendly.					
31013774	1	29	theme	building	135:142	arg1	materials					144:152	building materials	135:152	building materials	135:152	In order to meet the requirement of sustainable development, building materials are increasingly environmentally friendly.					
31013774	4	30	theme	previous	419:426	arg1	work					428:431	a previous work	417:431	a previous work	417:431	In a previous work, a feasibility study simultaneously investigated the processing and ratio of wheat straw required to ensure a gluing effect.					
31013774	0	31	dep	Composites	11:20	arg1	Study					30:34	Gluing Study	23:34	Gluing Study	23:34	Hemp-Straw Composites: Gluing Study and Multi-Physical Characterizations.					
31013774	0	31	dep	Composites	11:20	arg1	Characterizations					55:71	Multi-Physical Characterizations	40:71	Multi-Physical Characterizations	40:71	Hemp-Straw Composites: Gluing Study and Multi-Physical Characterizations.					
31013774	3	32	theme	bio-based	352:360	arg1	hemp					374:377	hemp	374:377	hemp	374:377	This paper looks at the development of fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat).					
31013774	3	32	theme	bio-based	352:360	arg1	aggregates					362:371	bio-based aggregates	352:371	bio-based aggregates (hemp)	352:378	This paper looks at the development of fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat).					
31013774	3	32	theme	bio-based	352:360	arg1	agro-resources					323:336	agro-resources	323:336	agro-resources	323:336	This paper looks at the development of fully bio-based composites where agro-resources are valued as bio-based aggregates (hemp) and as binding materials (wheat).					
31013774	9	33	theme	m	977:977	arg1	K					981:981	m · K	977:981	m · K	977:981	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	9	33	theme	m	977:977	arg1	mW/					973:975	67.9-69.0 mW/	963:975	67.9-69.0 mW/(m · K) at 23 ° C	963:992	Furthermore, they showed good thermal performances with a low thermal conductivity (67.9-69.0 mW/(m · K) at 23 ° C, dry).					
31013774	8	34	theme	insulating	857:866	arg1	materials					868:876	insulating materials	857:876	insulating materials	857:876	They showed sufficiently high mechanical properties to be used as insulating materials.					
31013774	7	35	theme	developed	733:741	arg1	composites					743:752	The developed composites	729:752	The developed composites	729:752	The developed composites were characterized multi-physically.					
30167743	2	0	theme	time	582:585	arg1	chemistry					541:549	chemistry	541:549	chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica	541:640	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	1	1	used	used	390:393	arg2	sorbent					248:254	A hierarchically porous structured composite monolith sorbent	194:254	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan)	194:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	1	used	used	390:393	arg2	extraction					410:419	solid-phase extraction	398:419	solid-phase extraction sorbent for the determination of carbamate pesticides	398:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	7	2	theme	chemical	1589:1596	arg1	stability					1598:1606	the good physical and chemical stability	1567:1606	the good physical and chemical stability of the sorbent	1567:1621	Extraction and clean-up were highly efficient, and the good physical and chemical stability of the sorbent enables reuse up to 13 times.					
30167743	7	3	dep	13	1643:1644	arg1	to					1640:1641	to	1640:1641	to	1640:1641	Extraction and clean-up were highly efficient, and the good physical and chemical stability of the sorbent enables reuse up to 13 times.					
30167743	3	4	theme	wide	964:967	arg1	responses					976:984	wide linear responses	964:984	wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb	964:1120	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	5	5	theme	composite	1231:1239	arg1	sorbent					1250:1256	the composite monolith sorbent	1227:1256	the composite monolith sorbent	1227:1256	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	2	6	theme	polymerization	567:580	arg1	time					582:585	polymerization time	567:585	polymerization time	567:585	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	1	7	theme	sorbent	421:427	arg1	extraction					410:419	solid-phase extraction	398:419	solid-phase extraction sorbent for the determination of carbamate pesticides	398:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	7	theme	sorbent	421:427	arg1	sorbent					248:254	A hierarchically porous structured composite monolith sorbent	194:254	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan)	194:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	5	8	theme	fruit	1330:1334	arg1	juices					1336:1341	fruit juices	1330:1341	fruit juices	1330:1341	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	4	9	theme	detection	1137:1145	arg1	limits					1127:1132	The limits	1123:1132	The limits of detection using HPLC-UV at 203, 220, and 208 nm	1123:1183	The limits of detection using HPLC-UV at 203, 220, and 208 nm were in the range of 0.5-2.0 μg L-1.					
30167743	3	10	theme	monolith	938:945	arg1	sorbent					947:953	the developed composite monolith sorbent	914:953	the developed composite monolith sorbent	914:953	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	3	11	theme	extraction	902:911	arg1	conditions					864:873	the optimal conditions	852:873	the optimal conditions of sorbent preparation and extraction	852:911	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	2	12	theme	prepared	683:690	arg1	sorbents					692:699	the prepared sorbents	679:699	the prepared sorbents	679:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	5	13	theme	carbamate	1317:1325	arg1	determination					1300:1312	determination	1300:1312	determination of carbamate	1300:1325	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	5	13	theme	carbamate	1317:1325	arg1	pre-concentration					1278:1294	pre-concentration	1278:1294	pre-concentration	1278:1294	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	1	14	theme	chitosan	330:337	arg1	PPY/GOx/C18/chitosan					348:367	PPY/GOx/C18/chitosan	348:367	PPY/GOx/C18/chitosan	348:367	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	14	theme	chitosan	330:337	arg1	cryogel					339:345	chitosan cryogel	330:345	chitosan cryogel (PPY/GOx/C18/chitosan)	330:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	2	15	theme	oxide	615:619	arg1	concentrations					588:601	concentrations	588:601	concentrations of graphene oxide	588:619	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	15	theme	oxide	615:619	arg1	sorbent					558:564	the sorbent	554:564	the sorbent	554:564	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	15	theme	oxide	615:619	arg1	silica					635:640	octadecyl silica	625:640	octadecyl silica	625:640	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	15	theme	oxide	615:619	arg1	time					582:585	polymerization time	567:585	polymerization time	567:585	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	16	theme	desorption	726:735	arg1	conditions					737:746	desorption conditions	726:746	desorption conditions	726:746	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	16	theme	desorption	726:735	arg1	factors					484:490	Various factors	476:490	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents	476:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	16	theme	desorption	726:735	arg1	loading					717:723	sample loading	710:723	sample loading	710:723	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	3	17	theme	preparation	886:896	arg1	conditions					864:873	the optimal conditions	852:873	the optimal conditions of sorbent preparation and extraction	852:911	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	2	18	theme	graphene	606:613	arg1	oxide					615:619	graphene oxide	606:619	graphene oxide	606:619	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	19	dep	adsorbents	529:538	arg1	chemistry					541:549	chemistry	541:549	chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica	541:640	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	3	20	from	500 μg L-1	1055:1064	arg1	responses					976:984	wide linear responses	964:984	wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb	964:1120	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	1	21	theme	porous	211:216	arg1	extraction					410:419	solid-phase extraction	398:419	solid-phase extraction sorbent for the determination of carbamate pesticides	398:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	21	theme	porous	211:216	arg1	sorbent					248:254	A hierarchically porous structured composite monolith sorbent	194:254	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan)	194:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	8	22	theme	Graphical	1653:1661	arg1	abstract					1663:1670	Graphical abstract	1653:1670	Graphical abstract	1653:1670	Graphical abstract ᅟ.					
30167743	2	23	theme	sample	710:715	arg1	loading					717:723	sample loading	710:723	sample loading	710:723	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	24	theme	sample	764:769	arg1	factors					484:490	Various factors	476:490	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents	476:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	24	theme	sample	764:769	arg1	rate					776:779	sample flow rate	764:779	sample flow rate	764:779	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	24	theme	sample	764:769	arg1	loading					717:723	sample loading	710:723	sample loading	710:723	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	0	25	dep	extraction	120:129	arg1	the					116:118	the	116:118	the	116:118	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	3	26	theme	linear	969:974	arg1	responses					976:984	wide linear responses	964:984	wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb	964:1120	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	1	27	theme	structured	218:227	arg1	extraction					410:419	solid-phase extraction	398:419	solid-phase extraction sorbent for the determination of carbamate pesticides	398:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	27	theme	structured	218:227	arg1	sorbent					248:254	A hierarchically porous structured composite monolith sorbent	194:254	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan)	194:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	2	28	theme	sorbent	558:564	arg1	chemistry					541:549	chemistry	541:549	chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica	541:640	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	1	29	theme	carbamate	454:462	arg1	pesticides					464:473	carbamate pesticides	454:473	carbamate pesticides	454:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	7	30	theme	physical	1576:1583	arg1	stability					1598:1606	the good physical and chemical stability	1567:1606	the good physical and chemical stability of the sorbent	1567:1621	Extraction and clean-up were highly efficient, and the good physical and chemical stability of the sorbent enables reuse up to 13 times.					
30167743	5	31	from	pre-concentration	1278:1294	arg1	juices					1336:1341	fruit juices	1330:1341	fruit juices	1330:1341	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	1	32	theme	composite	229:237	arg1	extraction					410:419	solid-phase extraction	398:419	solid-phase extraction sorbent for the determination of carbamate pesticides	398:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	32	theme	composite	229:237	arg1	sorbent					248:254	A hierarchically porous structured composite monolith sorbent	194:254	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan)	194:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	2	33	theme	silica	635:640	arg1	chemistry					541:549	chemistry	541:549	chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica	541:640	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	7	34	theme	good	1571:1574	arg1	stability					1598:1606	the good physical and chemical stability	1567:1606	the good physical and chemical stability of the sorbent	1567:1621	Extraction and clean-up were highly efficient, and the good physical and chemical stability of the sorbent enables reuse up to 13 times.					
30167743	5	35	theme	good	1344:1347	arg1	recoveries					1349:1358	good recoveries	1344:1358	good recoveries (84.1-99.5%)	1344:1371	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	5	35	theme	good	1344:1347	arg1	%					1370:1370	84.1-99.5%	1361:1370	84.1-99.5%	1361:1370	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	1	36	theme	monolith	239:246	arg1	extraction					410:419	solid-phase extraction	398:419	solid-phase extraction sorbent for the determination of carbamate pesticides	398:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	36	theme	monolith	239:246	arg1	sorbent					248:254	A hierarchically porous structured composite monolith sorbent	194:254	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan)	194:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	0	37	theme	composite	24:32	arg1	silica/graphene					65:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene	0:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene	0:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	0	38	theme	pesticides	166:175	arg1	pre-concentration					135:151	pre-concentration	135:151	pre-concentration	135:151	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	0	38	theme	pesticides	166:175	arg1	extraction					120:129	extraction	120:129	extraction	120:129	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	2	39	theme	sample	749:754	arg1	volume					756:761	sample volume	749:761	sample volume	749:761	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	39	theme	sample	749:754	arg1	factors					484:490	Various factors	476:490	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents	476:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	39	theme	sample	749:754	arg1	loading					717:723	sample loading	710:723	sample loading	710:723	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	3	40	theme	sorbent	878:884	arg1	preparation					886:896	sorbent preparation	878:896	sorbent preparation	878:896	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	0	41	theme	porous	17:22	arg1	silica/graphene					65:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene	0:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene	0:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	0	42	theme	carbamate	156:164	arg1	pesticides					166:175	carbamate pesticides	156:175	carbamate pesticides	156:175	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	1	43	theme	pesticides	464:473	arg1	determination					437:449	the determination	433:449	the determination of carbamate pesticides	433:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	3	44	dep	500 μg L-1	1055:1064	arg1	to					1052:1053	to	1052:1053	to	1052:1053	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	7	45	theme	sorbent	1615:1621	arg1	stability					1598:1606	the good physical and chemical stability	1567:1606	the good physical and chemical stability of the sorbent	1567:1621	Extraction and clean-up were highly efficient, and the good physical and chemical stability of the sorbent enables reuse up to 13 times.					
30167743	0	46	theme	polypyrrole/octadecyl	43:63	arg1	silica/graphene					65:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene	0:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene	0:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	5	47	theme	monolith	1241:1248	arg1	sorbent					1250:1256	the composite monolith sorbent	1227:1256	the composite monolith sorbent	1227:1256	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	0	48	theme	fruit	180:184	arg1	juices					186:191	fruit juices	180:191	fruit juices	180:191	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	5	49	dep	pre-concentration	1278:1294	arg1	the					1274:1276	the	1274:1276	the	1274:1276	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	1	50	theme	polypyrrole-coated	259:276	arg1	oxide					287:291	polypyrrole-coated graphene oxide	259:291	polypyrrole-coated graphene oxide	259:291	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	0	51	theme	monolith	34:41	arg1	silica/graphene					65:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene	0:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene	0:79	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	2	52	theme	octadecyl	625:633	arg1	silica					635:640	octadecyl silica	625:640	octadecyl silica	625:640	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	0	53	from	sorbent	104:110	arg1	juices					186:191	fruit juices	180:191	fruit juices	180:191	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	2	54	theme	adsorbents	529:538	arg1	characteristics					506:520	the characteristics	502:520	the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents	502:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	55	theme	sample	782:787	arg1	factors					484:490	Various factors	476:490	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents	476:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	55	theme	sample	782:787	arg1	pH					789:790	sample pH	782:790	sample pH	782:790	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	55	theme	sample	782:787	arg1	loading					717:723	sample loading	710:723	sample loading	710:723	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	1	56	theme	graphene	278:285	arg1	oxide					287:291	polypyrrole-coated graphene oxide	259:291	polypyrrole-coated graphene oxide	259:291	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	3	57	from	500 μg L-1	998:1007	arg1	responses					976:984	wide linear responses	964:984	wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb	964:1120	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	4	58	theme	0.5-2.0 μg L-1	1206:1219	arg1	range					1197:1201	the range	1193:1201	the range of 0.5-2.0 μg L-1	1193:1219	The limits of detection using HPLC-UV at 203, 220, and 208 nm were in the range of 0.5-2.0 μg L-1.					
30167743	3	59	from	2.0	1089:1091	arg1	responses					976:984	wide linear responses	964:984	wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb	964:1120	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	6	60	theme	low	1437:1439	arg1	pressure					1446:1453	low back pressure	1437:1453	low back pressure	1437:1453	The developed sorbents were porous and exhibited low back pressure enabling their use at high flow rates during sample loading.					
30167743	2	61	theme	efficiency	662:671	arg1	characteristics					506:520	the characteristics	502:520	the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents	502:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	3	62	theme	developed	918:926	arg1	sorbent					947:953	the developed composite monolith sorbent	914:953	the developed composite monolith sorbent	914:953	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	2	63	theme	extraction	651:660	arg1	efficiency					662:671	the extraction efficiency	647:671	the extraction efficiency using the prepared sorbents	647:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	64	theme	flow	771:774	arg1	factors					484:490	Various factors	476:490	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents	476:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	64	theme	flow	771:774	arg1	rate					776:779	sample flow rate	764:779	sample flow rate	764:779	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	64	theme	flow	771:774	arg1	loading					717:723	sample loading	710:723	sample loading	710:723	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	3	65	theme	composite	928:936	arg1	sorbent					947:953	the developed composite monolith sorbent	914:953	the developed composite monolith sorbent	914:953	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	1	66	theme	oxide	287:291	arg1	extraction					410:419	solid-phase extraction	398:419	solid-phase extraction sorbent for the determination of carbamate pesticides	398:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	66	theme	oxide	287:291	arg1	sorbent					248:254	A hierarchically porous structured composite monolith sorbent	194:254	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan)	194:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	5	67	from	determination	1300:1312	arg1	juices					1336:1341	fruit juices	1330:1341	fruit juices	1330:1341	When the composite monolith sorbent was applied for the pre-concentration and determination of carbamate in fruit juices, good recoveries (84.1-99.5%) were achieved.					
30167743	2	68	theme	ionic	797:801	arg1	strength					803:810	ionic strength	797:810	ionic strength	797:810	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	68	theme	ionic	797:801	arg1	factors					484:490	Various factors	476:490	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents	476:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	68	theme	ionic	797:801	arg1	loading					717:723	sample loading	710:723	sample loading	710:723	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	6	69	theme	developed	1392:1400	arg1	porous					1416:1421	porous	1416:1421	porous	1416:1421	The developed sorbents were porous and exhibited low back pressure enabling their use at high flow rates during sample loading.					
30167743	6	69	theme	developed	1392:1400	arg1	sorbents					1402:1409	The developed sorbents	1388:1409	The developed sorbents	1388:1409	The developed sorbents were porous and exhibited low back pressure enabling their use at high flow rates during sample loading.					
30167743	2	70	theme	concentrations	588:601	arg1	chemistry					541:549	chemistry	541:549	chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica	541:640	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	6	71	theme	flow	1482:1485	arg1	rates					1487:1491	high flow rates	1477:1491	high flow rates during sample loading	1477:1513	The developed sorbents were porous and exhibited low back pressure enabling their use at high flow rates during sample loading.					
30167743	3	72	dep	500 μg L-1	998:1007	arg1	to					995:996	to	995:996	to	995:996	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	2	73	theme	Various	476:482	arg1	factors					484:490	Various factors	476:490	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents	476:699	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	73	theme	Various	476:482	arg1	conditions					737:746	desorption conditions	726:746	desorption conditions	726:746	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	73	theme	Various	476:482	arg1	rate					776:779	sample flow rate	764:779	sample flow rate	764:779	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	73	theme	Various	476:482	arg1	strength					803:810	ionic strength	797:810	ionic strength	797:810	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	73	theme	Various	476:482	arg1	loading					717:723	sample loading	710:723	sample loading	710:723	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	73	theme	Various	476:482	arg1	volume					756:761	sample volume	749:761	sample volume	749:761	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	2	73	theme	Various	476:482	arg1	pH					789:790	sample pH	782:790	sample pH	782:790	Various factors affecting the characteristics of the adsorbents (chemistry of the sorbent, polymerization time, concentrations of graphene oxide and octadecyl silica) and the extraction efficiency using the prepared sorbents, such as sample loading, desorption conditions, sample volume, sample flow rate, sample pH, and ionic strength, were investigated and optimized.					
30167743	1	74	theme	octadecyl	297:305	arg1	silica					307:312	octadecyl silica	297:312	octadecyl silica	297:312	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	0	75	theme	cryogel	96:102	arg1	sorbent					104:110	cryogel sorbent	96:110	cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices	96:191	A hierarchically porous composite monolith polypyrrole/octadecyl silica/graphene oxide/chitosan cryogel sorbent for the extraction and pre-concentration of carbamate pesticides in fruit juices.					
30167743	1	76	theme	solid-phase	398:408	arg1	extraction					410:419	solid-phase extraction	398:419	solid-phase extraction sorbent for the determination of carbamate pesticides	398:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	76	theme	solid-phase	398:408	arg1	sorbent					248:254	A hierarchically porous structured composite monolith sorbent	194:254	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan)	194:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	3	77	theme	optimal	856:862	arg1	conditions					864:873	the optimal conditions	852:873	the optimal conditions of sorbent preparation and extraction	852:911	Under the optimal conditions of sorbent preparation and extraction, the developed composite monolith sorbent provided wide linear responses from 1.0 to 500 μg L-1 for carbofuran and diethofencarb, from 0.5 to 500 μg L-1 for carbaryl, and from 2.0 to 500 μg L-1 for isoprocarb.					
30167743	6	78	theme	high	1477:1480	arg1	rates					1487:1491	high flow rates	1477:1491	high flow rates during sample loading	1477:1513	The developed sorbents were porous and exhibited low back pressure enabling their use at high flow rates during sample loading.					
30167743	6	79	theme	sample	1500:1505	arg1	loading					1507:1513	sample loading	1500:1513	sample loading	1500:1513	The developed sorbents were porous and exhibited low back pressure enabling their use at high flow rates during sample loading.					
30167743	1	80	theme	silica	307:312	arg1	extraction					410:419	solid-phase extraction	398:419	solid-phase extraction sorbent for the determination of carbamate pesticides	398:473	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
30167743	1	80	theme	silica	307:312	arg1	sorbent					248:254	A hierarchically porous structured composite monolith sorbent	194:254	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan)	194:368	A hierarchically porous structured composite monolith sorbent of polypyrrole-coated graphene oxide and octadecyl silica incorporated in chitosan cryogel (PPY/GOx/C18/chitosan) was synthesized and used as solid-phase extraction sorbent for the determination of carbamate pesticides.					
29381716	1	0	theme	disc	236:239	arg1	degeneration					247:258	intervertebral disc (IVD) degeneration	221:258	intervertebral disc (IVD) degeneration	221:258	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	13	1	theme	altered	1743:1749	arg1	biomechanics					1751:1762	altered biomechanics	1743:1762	altered biomechanics	1743:1762	T1rho nucleus values correlated strongest with altered biomechanics, histological degeneration score, and loss of GAGs.					
29381716	1	2	theme	IVD	242:244	arg1	degeneration					247:258	intervertebral disc (IVD) degeneration	221:258	intervertebral disc (IVD) degeneration	221:258	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	4	3	theme	IVD	762:764	arg1	model					774:778	a graded degenerated lumbar IVD caprine model	734:778	a graded degenerated lumbar IVD caprine model	734:778	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	8	4	theme	T	1142:1142	arg1	images					1147:1152	High-resolution 9.4 T MR images	1122:1152	High-resolution 9.4 T MR images	1122:1152	High-resolution 9.4 T MR images were obtained prior to intervention and after culture.					
29381716	13	5	theme	nucleus	1702:1708	arg1	values					1710:1715	T1rho nucleus values	1696:1715	T1rho nucleus values	1696:1715	T1rho nucleus values correlated strongest with altered biomechanics, histological degeneration score, and loss of GAGs.					
29381716	11	6	theme	MR	1479:1480	arg1	sequences					1482:1490	All MR sequences	1475:1490	All MR sequences	1475:1490	All MR sequences detected changes in matrix composition, with T1rho showing largest changes pre-to-post in the nucleus, and significantly more than T2 and ADC.					
29381716	3	7	theme	MRI	469:471	arg1	techniques					473:482	Several quantitative MRI techniques	448:482	Several quantitative MRI techniques	448:482	Several quantitative MRI techniques have been introduced to detect changes in matrix composition signifying early DDD.					
29381716	4	8	theme	T2	609:610	arg1	values					660:665	quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values	596:665	quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values	596:665	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	5	9	dep	U/ml	903:906	arg1	ABC					923:925	Chondroïtinase ABC	908:925	0.5 U/ml Chondroïtinase ABC (Cabc)	899:932	METHODS Lumbar caprine IVDs were injected with either 0.25 U/ml or 0.5 U/ml Chondroïtinase ABC (Cabc) to trigger early DDD-like degeneration.					
29381716	11	10	from	changes	1501:1507	arg1	composition					1519:1529	matrix composition	1512:1529	matrix composition	1512:1529	All MR sequences detected changes in matrix composition, with T1rho showing largest changes pre-to-post in the nucleus, and significantly more than T2 and ADC.					
29381716	3	11	theme	composition	533:543	arg1	DDD					562:564	matrix composition signifying early DDD	526:564	matrix composition signifying early DDD	526:564	Several quantitative MRI techniques have been introduced to detect changes in matrix composition signifying early DDD.					
29381716	8	12	theme	High-resolution	1122:1136	arg1	images					1147:1152	High-resolution 9.4 T MR images	1122:1152	High-resolution 9.4 T MR images	1122:1152	High-resolution 9.4 T MR images were obtained prior to intervention and after culture.					
29381716	4	13	theme	Coefficient	642:652	arg1	values					660:665	quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values	596:665	quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values	596:665	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	0	14	theme	matrix	133:138	arg1	content					140:146	matrix content	133:146	matrix content	133:146	Quantitative MRI in early intervertebral disc degeneration: T1rho correlates better than T2 and ADC with biomechanics, histology and matrix content.					
29381716	1	15	attach	presence	209:216	arg2	degeneration					247:258	intervertebral disc (IVD) degeneration	221:258	intervertebral disc (IVD) degeneration	221:258	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	1	15	attach	presence	209:216	arg1	MRI					281:283	T2-weighted (T2w) MRI	263:283	T2-weighted (T2w) MRI	263:283	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	4	16	theme	DDD	817:819	arg1	detection					821:829	early DDD detection	811:829	early DDD detection	811:829	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	14	17	theme	T1rho	1836:1840	arg1	MR-mapping					1855:1864	T1rho quantitative MR-mapping	1836:1864	T1rho quantitative MR-mapping	1836:1864	CONCLUSIONS T2- and T1rho quantitative MR-mapping detected early DDD changes.					
29381716	14	17	theme	T1rho	1836:1840	arg1	CONCLUSIONS					1816:1826	CONCLUSIONS T2- and T1rho quantitative MR-mapping	1816:1864	CONCLUSIONS T2- and T1rho quantitative MR-mapping	1816:1864	CONCLUSIONS T2- and T1rho quantitative MR-mapping detected early DDD changes.					
29381716	5	18	theme	early	945:949	arg1	degeneration					960:971	early DDD-like degeneration	945:971	early DDD-like degeneration	945:971	METHODS Lumbar caprine IVDs were injected with either 0.25 U/ml or 0.5 U/ml Chondroïtinase ABC (Cabc) to trigger early DDD-like degeneration.					
29381716	4	19	from	markers	723:729	arg1	model					774:778	a graded degenerated lumbar IVD caprine model	734:778	a graded degenerated lumbar IVD caprine model	734:778	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	5	20	theme	Lumbar	840:845	arg1	IVDs					855:858	Lumbar caprine IVDs	840:858	Lumbar caprine IVDs	840:858	METHODS Lumbar caprine IVDs were injected with either 0.25 U/ml or 0.5 U/ml Chondroïtinase ABC (Cabc) to trigger early DDD-like degeneration.					
29381716	4	21	theme	T1rho	613:617	arg1	values					660:665	quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values	596:665	quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values	596:665	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	3	22	theme	early	556:560	arg1	DDD					562:564	matrix composition signifying early DDD	526:564	matrix composition signifying early DDD	526:564	Several quantitative MRI techniques have been introduced to detect changes in matrix composition signifying early DDD.					
29381716	16	23	theme	Clinical	2000:2007	arg1	implementation					2009:2022	Clinical implementation	2000:2022	Clinical implementation of quantitative MRI	2000:2042	Clinical implementation of quantitative MRI, T1rho particularly, could aid in distinguishing DDD more reliably at an earlier stage in the degenerative process.					
29381716	16	23	theme	Clinical	2000:2007	arg1	T1rho					2045:2049	T1rho	2045:2049	T1rho	2045:2049	Clinical implementation of quantitative MRI, T1rho particularly, could aid in distinguishing DDD more reliably at an earlier stage in the degenerative process.					
29381716	4	24	theme	Apparent	623:630	arg1	Coefficient					642:652	Apparent Diffusion Coefficient	623:652	Apparent Diffusion Coefficient (ADC)	623:658	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	4	24	theme	Apparent	623:630	arg1	ADC					655:657	ADC	655:657	ADC	655:657	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	5	25	dep	METHODS	832:838	arg1	injected					865:872	injected	865:872	were injected with either 0.25 U/ml or 0.5 U/ml Chondroïtinase ABC (Cabc) to trigger early DDD-like degeneration	860:971	METHODS Lumbar caprine IVDs were injected with either 0.25 U/ml or 0.5 U/ml Chondroïtinase ABC (Cabc) to trigger early DDD-like degeneration.					
29381716	7	26	theme	axial	1093:1097	arg1	loading					1113:1119	axial physiological loading	1093:1119	axial physiological loading	1093:1119	IVDs were cultured in a bioreactor for 20 days under axial physiological loading.					
29381716	4	27	from	behavior	686:693	arg1	model					774:778	a graded degenerated lumbar IVD caprine model	734:778	a graded degenerated lumbar IVD caprine model	734:778	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	16	28	theme	MRI	2040:2042	arg1	implementation					2009:2022	Clinical implementation	2000:2022	Clinical implementation of quantitative MRI	2000:2042	Clinical implementation of quantitative MRI, T1rho particularly, could aid in distinguishing DDD more reliably at an earlier stage in the degenerative process.					
29381716	16	28	theme	MRI	2040:2042	arg1	T1rho					2045:2049	T1rho	2045:2049	T1rho	2045:2049	Clinical implementation of quantitative MRI, T1rho particularly, could aid in distinguishing DDD more reliably at an earlier stage in the degenerative process.					
29381716	4	29	theme	gold	699:702	arg1	markers					723:729	gold standard early DDD markers	699:729	gold standard early DDD markers	699:729	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	2	30	theme	disc	352:355	arg1	DDD					366:368	DDD	366:368	DDD	366:368	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	2	30	theme	disc	352:355	arg1	disease					357:363	stage degenerative disc disease	333:363	stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD	333:445	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	9	31	theme	MR	1222:1223	arg1	results					1225:1231	Quantitative MR results	1209:1231	Quantitative MR results	1209:1231	Quantitative MR results were correlated to recovery behavior, histological degeneration grading, and the content of glycosaminoglycans (GAGs) and water.					
29381716	16	32	theme	degenerative	2138:2149	arg1	process					2151:2157	the degenerative process	2134:2157	the degenerative process	2134:2157	Clinical implementation of quantitative MRI, T1rho particularly, could aid in distinguishing DDD more reliably at an earlier stage in the degenerative process.					
29381716	1	33	theme	T2-weighted	263:273	arg1	MRI					281:283	T2-weighted (T2w) MRI	263:283	T2-weighted (T2w) MRI	263:283	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	2	34	theme	stage	333:337	arg1	DDD					366:368	DDD	366:368	DDD	366:368	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	2	34	theme	stage	333:337	arg1	disease					357:363	stage degenerative disc disease	333:363	stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD	333:445	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	0	35	theme	Quantitative	0:11	arg1	MRI					13:15	Quantitative MRI	0:15	Quantitative MRI in early intervertebral disc degeneration: T1rho correlates better than T2 and ADC with biomechanics, histology and matrix content.	0:147	Quantitative MRI in early intervertebral disc degeneration: T1rho correlates better than T2 and ADC with biomechanics, histology and matrix content.					
29381716	16	36	from	stage	2125:2129	arg1	process					2151:2157	the degenerative process	2134:2157	the degenerative process	2134:2157	Clinical implementation of quantitative MRI, T1rho particularly, could aid in distinguishing DDD more reliably at an earlier stage in the degenerative process.					
29381716	11	37	from	nucleus	1586:1592	arg1	pre-to-post					1567:1577	pre-to-post	1567:1577	pre-to-post	1567:1577	All MR sequences detected changes in matrix composition, with T1rho showing largest changes pre-to-post in the nucleus, and significantly more than T2 and ADC.					
29381716	4	38	theme	disc	670:673	arg1	behavior					686:693	disc mechanical behavior	670:693	disc mechanical behavior	670:693	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	10	39	from	changes	1449:1455	arg1	water-content					1460:1472	water-content	1460:1472	water-content	1460:1472	RESULTS Cabc-injected IVDs showed aberrancies in biomechanics and loss of GAGs without changes in water-content.					
29381716	2	40	theme	IVD	408:410	arg1	height					412:417	IVD height	408:417	IVD height	408:417	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	13	41	theme	histological	1765:1776	arg1	score					1791:1795	histological degeneration score	1765:1795	histological degeneration score	1765:1795	T1rho nucleus values correlated strongest with altered biomechanics, histological degeneration score, and loss of GAGs.					
29381716	0	42	theme	intervertebral	26:39	arg1	degeneration					46:57	early intervertebral disc degeneration	20:57	early intervertebral disc degeneration	20:57	Quantitative MRI in early intervertebral disc degeneration: T1rho correlates better than T2 and ADC with biomechanics, histology and matrix content.					
29381716	10	43	theme	GAGs	1436:1439	arg1	loss					1428:1431	loss	1428:1431	loss of GAGs	1428:1439	RESULTS Cabc-injected IVDs showed aberrancies in biomechanics and loss of GAGs without changes in water-content.					
29381716	10	43	theme	GAGs	1436:1439	arg1	aberrancies					1396:1406	aberrancies	1396:1406	aberrancies in biomechanics	1396:1422	RESULTS Cabc-injected IVDs showed aberrancies in biomechanics and loss of GAGs without changes in water-content.					
29381716	4	44	theme	degenerated	743:753	arg1	model					774:778	a graded degenerated lumbar IVD caprine model	734:778	a graded degenerated lumbar IVD caprine model	734:778	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	11	45	theme	matrix	1512:1517	arg1	composition					1519:1529	matrix composition	1512:1529	matrix composition	1512:1529	All MR sequences detected changes in matrix composition, with T1rho showing largest changes pre-to-post in the nucleus, and significantly more than T2 and ADC.					
29381716	9	46	theme	histological	1271:1282	arg1	grading					1297:1303	histological degeneration grading	1271:1303	histological degeneration grading	1271:1303	Quantitative MR results were correlated to recovery behavior, histological degeneration grading, and the content of glycosaminoglycans (GAGs) and water.					
29381716	14	47	theme	DDD	1881:1883	arg1	changes					1885:1891	early DDD changes	1875:1891	early DDD changes	1875:1891	CONCLUSIONS T2- and T1rho quantitative MR-mapping detected early DDD changes.					
29381716	15	48	theme	nucleus	1900:1906	arg1	values					1908:1913	T1rho nucleus values	1894:1913	T1rho nucleus values	1894:1913	T1rho nucleus values correlated better than T2 and ADC with biomechanical, histological, and GAG changes.					
29381716	4	49	theme	early	713:717	arg1	markers					723:729	gold standard early DDD markers	699:729	gold standard early DDD markers	699:729	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	5	50	theme	Chondroïtinase	908:921	arg1	ABC					923:925	Chondroïtinase ABC	908:925	0.5 U/ml Chondroïtinase ABC (Cabc)	899:932	METHODS Lumbar caprine IVDs were injected with either 0.25 U/ml or 0.5 U/ml Chondroïtinase ABC (Cabc) to trigger early DDD-like degeneration.					
29381716	16	51	theme	earlier	2117:2123	arg1	stage					2125:2129	an earlier stage	2114:2129	an earlier stage in the degenerative process	2114:2157	Clinical implementation of quantitative MRI, T1rho particularly, could aid in distinguishing DDD more reliably at an earlier stage in the degenerative process.					
29381716	11	52	theme	pre-to-post	1567:1577	arg1	changes					1559:1565	largest changes	1551:1565	largest changes pre-to-post in the nucleus	1551:1592	All MR sequences detected changes in matrix composition, with T1rho showing largest changes pre-to-post in the nucleus, and significantly more than T2 and ADC.					
29381716	1	53	theme	intervertebral	221:234	arg1	degeneration					247:258	intervertebral disc (IVD) degeneration	221:258	intervertebral disc (IVD) degeneration	221:258	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	12	54	theme	Cabc	1671:1674	arg1	injection					1676:1684	Cabc injection	1671:1684	Cabc injection	1671:1684	Histologically, degeneration due to Cabc injection was mild.					
29381716	6	55	theme	phosphate-buffered	989:1006	arg1	PBS					1016:1018	PBS	1016:1018	PBS	1016:1018	Injection with phosphate-buffered saline (PBS) served as control.					
29381716	6	55	theme	phosphate-buffered	989:1006	arg1	saline					1008:1013	phosphate-buffered saline	989:1013	phosphate-buffered saline (PBS)	989:1019	Injection with phosphate-buffered saline (PBS) served as control.					
29381716	11	56	theme	largest	1551:1557	arg1	changes					1559:1565	largest changes	1551:1565	largest changes pre-to-post in the nucleus	1551:1592	All MR sequences detected changes in matrix composition, with T1rho showing largest changes pre-to-post in the nucleus, and significantly more than T2 and ADC.					
29381716	9	57	theme	glycosaminoglycans	1325:1342	arg1	grading					1297:1303	histological degeneration grading	1271:1303	histological degeneration grading	1271:1303	Quantitative MR results were correlated to recovery behavior, histological degeneration grading, and the content of glycosaminoglycans (GAGs) and water.					
29381716	9	57	theme	glycosaminoglycans	1325:1342	arg1	water					1355:1359	water	1355:1359	water	1355:1359	Quantitative MR results were correlated to recovery behavior, histological degeneration grading, and the content of glycosaminoglycans (GAGs) and water.					
29381716	9	57	theme	glycosaminoglycans	1325:1342	arg1	content					1314:1320	the content	1310:1320	the content of glycosaminoglycans (GAGs)	1310:1349	Quantitative MR results were correlated to recovery behavior, histological degeneration grading, and the content of glycosaminoglycans (GAGs) and water.					
29381716	9	57	theme	glycosaminoglycans	1325:1342	arg1	behavior					1261:1268	recovery behavior	1252:1268	recovery behavior	1252:1268	Quantitative MR results were correlated to recovery behavior, histological degeneration grading, and the content of glycosaminoglycans (GAGs) and water.					
29381716	14	58	theme	quantitative	1842:1853	arg1	MR-mapping					1855:1864	T1rho quantitative MR-mapping	1836:1864	T1rho quantitative MR-mapping	1836:1864	CONCLUSIONS T2- and T1rho quantitative MR-mapping detected early DDD changes.					
29381716	14	58	theme	quantitative	1842:1853	arg1	CONCLUSIONS					1816:1826	CONCLUSIONS T2- and T1rho quantitative MR-mapping	1816:1864	CONCLUSIONS T2- and T1rho quantitative MR-mapping	1816:1864	CONCLUSIONS T2- and T1rho quantitative MR-mapping detected early DDD changes.					
29381716	13	59	theme	T1rho	1696:1700	arg1	values					1710:1715	T1rho nucleus values	1696:1715	T1rho nucleus values	1696:1715	T1rho nucleus values correlated strongest with altered biomechanics, histological degeneration score, and loss of GAGs.					
29381716	3	60	theme	quantitative	456:467	arg1	techniques					473:482	Several quantitative MRI techniques	448:482	Several quantitative MRI techniques	448:482	Several quantitative MRI techniques have been introduced to detect changes in matrix composition signifying early DDD.					
29381716	1	61	theme	Low-back	162:169	arg1	LBP					177:179	LBP	177:179	LBP	177:179	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	1	61	theme	Low-back	162:169	arg1	pain					171:174	Low-back pain	162:174	INTRODUCTION Low-back pain (LBP)	149:180	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	4	62	theme	lumbar	755:760	arg1	model					774:778	a graded degenerated lumbar IVD caprine model	734:778	a graded degenerated lumbar IVD caprine model	734:778	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	4	63	theme	caprine	766:772	arg1	model					774:778	a graded degenerated lumbar IVD caprine model	734:778	a graded degenerated lumbar IVD caprine model	734:778	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	4	64	theme	quantitative	596:607	arg1	values					660:665	quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values	596:665	quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values	596:665	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	16	65	theme	distinguishing	2078:2091	arg1	DDD					2093:2095	distinguishing DDD	2078:2095	distinguishing DDD	2078:2095	Clinical implementation of quantitative MRI, T1rho particularly, could aid in distinguishing DDD more reliably at an earlier stage in the degenerative process.					
29381716	3	66	theme	matrix	526:531	arg1	DDD					562:564	matrix composition signifying early DDD	526:564	matrix composition signifying early DDD	526:564	Several quantitative MRI techniques have been introduced to detect changes in matrix composition signifying early DDD.					
29381716	8	67	theme	MR	1144:1145	arg1	images					1147:1152	High-resolution 9.4 T MR images	1122:1152	High-resolution 9.4 T MR images	1122:1152	High-resolution 9.4 T MR images were obtained prior to intervention and after culture.					
29381716	1	68	from	presence	209:216	arg1	MRI					281:283	T2-weighted (T2w) MRI	263:283	T2-weighted (T2w) MRI	263:283	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	4	69	theme	Diffusion	632:640	arg1	Coefficient					642:652	Apparent Diffusion Coefficient	623:652	Apparent Diffusion Coefficient (ADC)	623:658	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	4	69	theme	Diffusion	632:640	arg1	ADC					655:657	ADC	655:657	ADC	655:657	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	10	70	from	aberrancies	1396:1406	arg1	biomechanics					1411:1422	biomechanics	1411:1422	biomechanics	1411:1422	RESULTS Cabc-injected IVDs showed aberrancies in biomechanics and loss of GAGs without changes in water-content.					
29381716	4	71	theme	early	811:815	arg1	detection					821:829	early DDD detection	811:829	early DDD detection	811:829	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	5	72	theme	DDD-like	951:958	arg1	degeneration					960:971	early DDD-like degeneration	945:971	early DDD-like degeneration	945:971	METHODS Lumbar caprine IVDs were injected with either 0.25 U/ml or 0.5 U/ml Chondroïtinase ABC (Cabc) to trigger early DDD-like degeneration.					
29381716	3	73	theme	signifying	545:554	arg1	DDD					562:564	matrix composition signifying early DDD	526:564	matrix composition signifying early DDD	526:564	Several quantitative MRI techniques have been introduced to detect changes in matrix composition signifying early DDD.					
29381716	7	74	theme	physiological	1099:1111	arg1	loading					1113:1119	axial physiological loading	1093:1119	axial physiological loading	1093:1119	IVDs were cultured in a bioreactor for 20 days under axial physiological loading.					
29381716	10	75	from	loss	1428:1431	arg1	biomechanics					1411:1422	biomechanics	1411:1422	biomechanics	1411:1422	RESULTS Cabc-injected IVDs showed aberrancies in biomechanics and loss of GAGs without changes in water-content.					
29381716	2	76	theme	height	412:417	arg1	measurements					392:403	measurements	392:403	measurements	392:403	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	2	76	theme	height	412:417	arg1	MRI					384:386	T2w MRI	380:386	T2w MRI	380:386	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	0	77	from	MRI	13:15	arg1	degeneration					46:57	early intervertebral disc degeneration	20:57	early intervertebral disc degeneration	20:57	Quantitative MRI in early intervertebral disc degeneration: T1rho correlates better than T2 and ADC with biomechanics, histology and matrix content.					
29381716	15	78	theme	T1rho	1894:1898	arg1	values					1908:1913	T1rho nucleus values	1894:1913	T1rho nucleus values	1894:1913	T1rho nucleus values correlated better than T2 and ADC with biomechanical, histological, and GAG changes.					
29381716	13	79	theme	GAGs	1810:1813	arg1	score					1791:1795	histological degeneration score	1765:1795	histological degeneration score	1765:1795	T1rho nucleus values correlated strongest with altered biomechanics, histological degeneration score, and loss of GAGs.					
29381716	13	79	theme	GAGs	1810:1813	arg1	loss					1802:1805	loss	1802:1805	loss of GAGs	1802:1813	T1rho nucleus values correlated strongest with altered biomechanics, histological degeneration score, and loss of GAGs.					
29381716	13	79	theme	GAGs	1810:1813	arg1	biomechanics					1751:1762	altered biomechanics	1743:1762	altered biomechanics	1743:1762	T1rho nucleus values correlated strongest with altered biomechanics, histological degeneration score, and loss of GAGs.					
29381716	15	80	theme	GAG	1987:1989	arg1	changes					1991:1997	biomechanical, histological, and GAG changes	1954:1997	changes	1991:1997	T1rho nucleus values correlated better than T2 and ADC with biomechanical, histological, and GAG changes.					
29381716	4	81	theme	mechanical	675:684	arg1	behavior					686:693	disc mechanical behavior	670:693	disc mechanical behavior	670:693	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	13	82	theme	degeneration	1778:1789	arg1	score					1791:1795	histological degeneration score	1765:1795	histological degeneration score	1765:1795	T1rho nucleus values correlated strongest with altered biomechanics, histological degeneration score, and loss of GAGs.					
29381716	9	83	theme	Quantitative	1209:1220	arg1	results					1225:1231	Quantitative MR results	1209:1231	Quantitative MR results	1209:1231	Quantitative MR results were correlated to recovery behavior, histological degeneration grading, and the content of glycosaminoglycans (GAGs) and water.					
29381716	2	84	theme	early	437:441	arg1	DDD					443:445	early DDD	437:445	early DDD	437:445	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	10	85	theme	Cabc-injected	1370:1382	arg1	IVDs					1384:1387	Cabc-injected IVDs	1370:1387	Cabc-injected IVDs	1370:1387	RESULTS Cabc-injected IVDs showed aberrancies in biomechanics and loss of GAGs without changes in water-content.					
29381716	2	86	theme	degenerative	339:350	arg1	DDD					366:368	DDD	366:368	DDD	366:368	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	2	86	theme	degenerative	339:350	arg1	disease					357:363	stage degenerative disc disease	333:363	stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD	333:445	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	10	87	dep	RESULTS	1362:1368	arg1	showed					1389:1394	showed	1389:1394	showed aberrancies in biomechanics and loss of GAGs without changes in water-content	1389:1472	RESULTS Cabc-injected IVDs showed aberrancies in biomechanics and loss of GAGs without changes in water-content.					
29381716	5	88	theme	caprine	847:853	arg1	IVDs					855:858	Lumbar caprine IVDs	840:858	Lumbar caprine IVDs	840:858	METHODS Lumbar caprine IVDs were injected with either 0.25 U/ml or 0.5 U/ml Chondroïtinase ABC (Cabc) to trigger early DDD-like degeneration.					
29381716	2	89	theme	T2w	380:382	arg1	MRI					384:386	T2w MRI	380:386	T2w MRI	380:386	It remains challenging, however, to accurately stage degenerative disc disease (DDD) based on T2w MRI and measurements of IVD height, particularly for early DDD.					
29381716	1	90	dep	INTRODUCTION	149:160	arg1	LBP					177:179	LBP	177:179	LBP	177:179	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	1	90	dep	INTRODUCTION	149:160	arg1	pain					171:174	Low-back pain	162:174	INTRODUCTION Low-back pain (LBP)	149:180	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	3	91	from	changes	515:521	arg1	DDD					562:564	matrix composition signifying early DDD	526:564	matrix composition signifying early DDD	526:564	Several quantitative MRI techniques have been introduced to detect changes in matrix composition signifying early DDD.					
29381716	0	92	theme	early	20:24	arg1	degeneration					46:57	early intervertebral disc degeneration	20:57	early intervertebral disc degeneration	20:57	Quantitative MRI in early intervertebral disc degeneration: T1rho correlates better than T2 and ADC with biomechanics, histology and matrix content.					
29381716	1	93	theme	degeneration	247:258	arg1	presence					209:216	the presence	205:216	the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI	205:283	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	14	94	dep	CONCLUSIONS	1816:1826	arg1	T2-					1828:1830	T2-	1828:1830	T2-	1828:1830	CONCLUSIONS T2- and T1rho quantitative MR-mapping detected early DDD changes.					
29381716	14	94	dep	CONCLUSIONS	1816:1826	arg1	MR-mapping					1855:1864	T1rho quantitative MR-mapping	1836:1864	T1rho quantitative MR-mapping	1836:1864	CONCLUSIONS T2- and T1rho quantitative MR-mapping detected early DDD changes.					
29381716	14	94	dep	CONCLUSIONS	1816:1826	arg1	CONCLUSIONS					1816:1826	CONCLUSIONS T2- and T1rho quantitative MR-mapping	1816:1864	CONCLUSIONS T2- and T1rho quantitative MR-mapping	1816:1864	CONCLUSIONS T2- and T1rho quantitative MR-mapping detected early DDD changes.					
29381716	0	95	theme	disc	41:44	arg1	degeneration					46:57	early intervertebral disc degeneration	20:57	early intervertebral disc degeneration	20:57	Quantitative MRI in early intervertebral disc degeneration: T1rho correlates better than T2 and ADC with biomechanics, histology and matrix content.					
29381716	9	96	theme	recovery	1252:1259	arg1	behavior					1261:1268	recovery behavior	1252:1268	recovery behavior	1252:1268	Quantitative MR results were correlated to recovery behavior, histological degeneration grading, and the content of glycosaminoglycans (GAGs) and water.					
29381716	1	97	theme	T2w	276:278	arg1	MRI					281:283	T2-weighted (T2w) MRI	263:283	T2-weighted (T2w) MRI	263:283	INTRODUCTION Low-back pain (LBP) has been correlated to the presence of intervertebral disc (IVD) degeneration on T2-weighted (T2w) MRI.					
29381716	6	98	with	Injection	974:982	arg1	PBS					1016:1018	PBS	1016:1018	PBS	1016:1018	Injection with phosphate-buffered saline (PBS) served as control.					
29381716	6	98	with	Injection	974:982	arg1	saline					1008:1013	phosphate-buffered saline	989:1013	phosphate-buffered saline (PBS)	989:1019	Injection with phosphate-buffered saline (PBS) served as control.					
29381716	11	99	from	pre-to-post	1567:1577	arg1	nucleus					1586:1592	the nucleus	1582:1592	the nucleus	1582:1592	All MR sequences detected changes in matrix composition, with T1rho showing largest changes pre-to-post in the nucleus, and significantly more than T2 and ADC.					
29381716	4	100	theme	graded	736:741	arg1	model					774:778	a graded degenerated lumbar IVD caprine model	734:778	a graded degenerated lumbar IVD caprine model	734:778	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	9	101	theme	degeneration	1284:1295	arg1	grading					1297:1303	histological degeneration grading	1271:1303	histological degeneration grading	1271:1303	Quantitative MR results were correlated to recovery behavior, histological degeneration grading, and the content of glycosaminoglycans (GAGs) and water.					
29381716	14	102	theme	early	1875:1879	arg1	changes					1885:1891	early DDD changes	1875:1891	early DDD changes	1875:1891	CONCLUSIONS T2- and T1rho quantitative MR-mapping detected early DDD changes.					
29381716	4	103	theme	standard	704:711	arg1	markers					723:729	gold standard early DDD markers	699:729	gold standard early DDD markers	699:729	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	0	104	dep	MRI	13:15	arg1	correlates					66:75	correlates	66:75	correlates better than T2 and ADC with biomechanics, histology and matrix content	66:146	Quantitative MRI in early intervertebral disc degeneration: T1rho correlates better than T2 and ADC with biomechanics, histology and matrix content.					
29381716	16	105	theme	quantitative	2027:2038	arg1	MRI					2040:2042	quantitative MRI	2027:2042	quantitative MRI	2027:2042	Clinical implementation of quantitative MRI, T1rho particularly, could aid in distinguishing DDD more reliably at an earlier stage in the degenerative process.					
29381716	4	106	theme	DDD	719:721	arg1	markers					723:729	gold standard early DDD markers	699:729	gold standard early DDD markers	699:729	In this study, we correlated quantitative T2, T1rho and Apparent Diffusion Coefficient (ADC) values to disc mechanical behavior and gold standard early DDD markers in a graded degenerated lumbar IVD caprine model, to assess their potential for early DDD detection.					
29381716	3	107	theme	Several	448:454	arg1	techniques					473:482	Several quantitative MRI techniques	448:482	Several quantitative MRI techniques	448:482	Several quantitative MRI techniques have been introduced to detect changes in matrix composition signifying early DDD.					
30845148	5	0	theme	alginate	1024:1031	arg1	films					1033:1037	zinc alginate films	1019:1037	zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites	1019:1127	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	8	1	theme	sodium	1469:1474	arg1	alginates					1476:1484	sodium alginates	1469:1484	sodium alginates	1469:1484	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	5	2	with	films	1033:1037	arg1	GO					1044:1045	GO	1044:1045	GO	1044:1045	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	0	3	from	oxide	9:13	arg1	films					32:36	zinc alginate films	18:36	zinc alginate films	18:36	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	5	4	theme	zinc	1019:1022	arg1	films					1033:1037	zinc alginate films	1019:1037	zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites	1019:1127	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	5	5	theme	films	1033:1037	arg1	synthesis					1006:1014	the synthesis	1002:1014	the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites	1002:1127	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	8	6	theme	different	1450:1458	arg1	types					1460:1464	different types	1450:1464	different types of sodium alginates	1450:1484	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	4	7	theme	divalent	802:809	arg1	cations					811:817	other divalent cations	796:817	other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+	796:882	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	4	7	theme	divalent	802:809	arg1	Zn2+					879:882	Zn2+	879:882	Zn2+	879:882	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	8	8	theme	GO	1422:1423	arg1	combination					1407:1417	the combination	1403:1417	the combination of GO with Zn2+	1403:1433	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	0	9	theme	zinc	77:80	arg1	release					82:88	zinc release	77:88	zinc release	77:88	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	4	10	theme	other	796:800	arg1	cations					811:817	other divalent cations	796:817	other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+	796:882	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	4	10	theme	other	796:800	arg1	Zn2+					879:882	Zn2+	879:882	Zn2+	879:882	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	3	11	from	reports	639:645	arg1	literature					650:659	literature	650:659	literature	650:659	However, many other reports in literature have shown controversial results about the toxicity of GO demanding further investigation.					
30845148	1	12	theme	low-cost	271:278	arg1	biomaterial					180:190	an exceptional biomaterial	165:190	an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost	165:278	Alginate is considered an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost in comparison with other biopolymers.					
30845148	0	13	theme	water	91:95	arg1	sorption/diffusion					97:114	water sorption/diffusion	91:114	water sorption/diffusion	91:114	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	6	14	theme	zinc	1168:1171	arg1	release					1173:1179	zinc release	1168:1179	zinc release	1168:1179	The results of this study showed that zinc release, water sorption/diffusion and wettability depended significantly on the type of alginate utilized.					
30845148	1	15	theme	exceptional	168:178	arg1	biomaterial					180:190	an exceptional biomaterial	165:190	an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost	165:278	Alginate is considered an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost in comparison with other biopolymers.					
30845148	0	16	theme	Graphene	0:7	arg1	oxide					9:13	Graphene oxide	0:13	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.	0:140	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	8	17	theme	antibacterial	1499:1511	arg1	activity					1513:1520	antibacterial activity	1499:1520	antibacterial activity	1499:1520	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	5	18	used	utilized	990:997	arg2	alginates					957:965	two commercially available sodium alginates	923:965	two commercially available sodium alginates	923:965	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	8	19	theme	Gram-positive	1575:1587	arg1	pathogens					1589:1597	these Gram-positive pathogens	1569:1597	these Gram-positive pathogens	1569:1597	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	7	20	theme	alginate	1314:1321	arg1	films					1323:1327	alginate films	1314:1327	alginate films	1314:1327	Furthermore, Zn2+ and GO produced alginate films with increased water diffusion, wettability and opacity.					
30845148	2	21	theme	oxide	386:390	arg1	incorporation					357:369	the incorporation	353:369	the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations	353:445	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	2	22	theme	Ca2+	434:437	arg1	cations					439:445	Ca2+ cations	434:445	Ca2+ cations	434:445	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	8	23	theme	human	1603:1607	arg1	cells					1609:1613	human cells	1603:1613	human cells	1603:1613	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	6	24	theme	water	1182:1186	arg1	sorption/diffusion					1188:1205	water sorption/diffusion	1182:1205	water sorption/diffusion	1182:1205	The results of this study showed that zinc release, water sorption/diffusion and wettability depended significantly on the type of alginate utilized.					
30845148	2	25	theme	graphene	377:384	arg1	oxide					386:390	1% graphene oxide	374:390	1% graphene oxide (GO)	374:395	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	0	26	theme	zinc	18:21	arg1	films					32:36	zinc alginate films	18:36	zinc alginate films	18:36	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	4	27	theme	cytotoxic	852:860	arg1	activity					862:869	intrinsic antibacterial and cytotoxic activity	824:869	intrinsic antibacterial and cytotoxic activity	824:869	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	2	28	theme	human	587:591	arg1	cells					612:616	human keratinocyte HaCaT cells	587:616	human keratinocyte HaCaT cells	587:616	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	1	29	with	comparison	283:292	arg1	biopolymers					305:315	other biopolymers	299:315	other biopolymers	299:315	Alginate is considered an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost in comparison with other biopolymers.					
30845148	1	30	theme	due	192:194	arg1	biomaterial					180:190	an exceptional biomaterial	165:190	an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost	165:278	Alginate is considered an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost in comparison with other biopolymers.					
30845148	8	31	dep	activity	1513:1520	arg1	the					1495:1497	the	1495:1497	the	1495:1497	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	5	32	theme	calcium	1098:1104	arg1	composites					1118:1127	the calcium alginate/GO composites	1094:1127	the calcium alginate/GO composites	1094:1127	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	4	33	theme	GO	788:789	arg1	effect					778:783	the synergic effect	765:783	the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+	765:882	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	2	34	theme	alginate	402:409	arg1	films					411:415	alginate films	402:415	alginate films crosslinked with Ca2+ cations	402:445	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	1	35	theme	other	299:303	arg1	biopolymers					305:315	other biopolymers	299:315	other biopolymers	299:315	Alginate is considered an exceptional biomaterial due to its hydrophilicity, biocompatibility, biodegradability, nontoxicity and low-cost in comparison with other biopolymers.					
30845148	5	36	theme	alginate/GO	1106:1116	arg1	composites					1118:1127	the calcium alginate/GO composites	1094:1127	the calcium alginate/GO composites	1094:1127	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	3	37	theme	many	628:631	arg1	reports					639:645	many other reports	628:645	many other reports in literature	628:659	However, many other reports in literature have shown controversial results about the toxicity of GO demanding further investigation.					
30845148	7	38	theme	increased	1334:1342	arg1	diffusion					1350:1358	increased water diffusion	1334:1358	increased water diffusion	1334:1358	Furthermore, Zn2+ and GO produced alginate films with increased water diffusion, wettability and opacity.					
30845148	0	39	theme	alginate	23:30	arg1	films					32:36	zinc alginate films	18:36	zinc alginate films	18:36	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	3	40	theme	other	633:637	arg1	reports					639:645	many other reports	628:645	many other reports in literature	628:659	However, many other reports in literature have shown controversial results about the toxicity of GO demanding further investigation.					
30845148	7	41	theme	water	1344:1348	arg1	diffusion					1350:1358	increased water diffusion	1334:1358	increased water diffusion	1334:1358	Furthermore, Zn2+ and GO produced alginate films with increased water diffusion, wettability and opacity.					
30845148	2	42	theme	antibacterial	456:468	arg1	activity					470:477	antibacterial activity	456:477	antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis	456:560	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	3	43	theme	controversial	672:684	arg1	results					686:692	controversial results	672:692	controversial results about the toxicity of GO demanding further investigation	672:749	However, many other reports in literature have shown controversial results about the toxicity of GO demanding further investigation.					
30845148	5	44	theme	same	1061:1064	arg1	route					1075:1079	the same chemical route	1057:1079	the same chemical route reported for the calcium alginate/GO composites	1057:1127	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	5	45	theme	available	940:948	arg1	alginates					957:965	two commercially available sodium alginates	923:965	two commercially available sodium alginates	923:965	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	8	46	theme	zinc	1546:1549	arg1	alginates					1551:1559	the zinc alginates	1542:1559	the zinc alginates	1542:1559	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	4	47	with	cations	811:817	arg1	activity					862:869	intrinsic antibacterial and cytotoxic activity	824:869	intrinsic antibacterial and cytotoxic activity	824:869	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	5	48	theme	chemical	1066:1073	arg1	route					1075:1079	the same chemical route	1057:1079	the same chemical route reported for the calcium alginate/GO composites	1057:1127	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	5	49	theme	sodium	950:955	arg1	alginates					957:965	two commercially available sodium alginates	923:965	two commercially available sodium alginates	923:965	Thus, here, two commercially available sodium alginates were characterised and utilized in the synthesis of zinc alginate films with GO following the same chemical route reported for the calcium alginate/GO composites.					
30845148	6	50	theme	alginate	1261:1268	arg1	type					1253:1256	the type	1249:1256	the type of alginate utilized	1249:1277	The results of this study showed that zinc release, water sorption/diffusion and wettability depended significantly on the type of alginate utilized.					
30845148	4	51	with	effect	778:783	arg1	cations					811:817	other divalent cations	796:817	other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+	796:882	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	4	51	with	effect	778:783	arg1	Zn2+					879:882	Zn2+	879:882	Zn2+	879:882	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	2	52	theme	HaCaT	606:610	arg1	cells					612:616	human keratinocyte HaCaT cells	587:616	human keratinocyte HaCaT cells	587:616	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	3	53	theme	GO	716:717	arg1	toxicity					704:711	the toxicity	700:711	the toxicity of GO demanding further investigation	700:749	However, many other reports in literature have shown controversial results about the toxicity of GO demanding further investigation.					
30845148	2	54	dep	oxide	386:390	arg1	GO					393:394	GO	393:394	GO	393:394	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	4	55	theme	synergic	769:776	arg1	effect					778:783	the synergic effect	765:783	the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+	765:882	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	2	56	theme	keratinocyte	593:604	arg1	cells					612:616	human keratinocyte HaCaT cells	587:616	human keratinocyte HaCaT cells	587:616	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	8	57	theme	alginates	1476:1484	arg1	types					1460:1464	different types	1450:1464	different types of sodium alginates	1450:1484	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	4	58	theme	intrinsic	824:832	arg1	activity					862:869	intrinsic antibacterial and cytotoxic activity	824:869	intrinsic antibacterial and cytotoxic activity	824:869	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	6	59	theme	study	1150:1154	arg1	results					1134:1140	The results	1130:1140	The results of this study	1130:1154	The results of this study showed that zinc release, water sorption/diffusion and wettability depended significantly on the type of alginate utilized.					
30845148	4	60	theme	antibacterial	834:846	arg1	activity					862:869	intrinsic antibacterial and cytotoxic activity	824:869	intrinsic antibacterial and cytotoxic activity	824:869	Furthermore, the synergic effect of GO with other divalent cations with intrinsic antibacterial and cytotoxic activity such as Zn2+ has not been explored yet.					
30845148	8	61	theme	alginates	1551:1559	arg1	activity					1513:1520	antibacterial activity	1499:1520	antibacterial activity	1499:1520	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	8	61	theme	alginates	1551:1559	arg1	cytotoxicity					1526:1537	cytotoxicity	1526:1537	cytotoxicity	1526:1537	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	0	62	dep	oxide	9:13	arg1	release					82:88	zinc release	77:88	zinc release	77:88	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	0	62	dep	oxide	9:13	arg1	opacity					133:139	opacity	133:139	opacity	133:139	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	0	62	dep	oxide	9:13	arg1	activity					53:60	Antibacterial activity	39:60	Antibacterial activity	39:60	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	0	62	dep	oxide	9:13	arg1	cytotoxicity					63:74	cytotoxicity	63:74	cytotoxicity	63:74	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	0	62	dep	oxide	9:13	arg1	sorption/diffusion					97:114	water sorption/diffusion	91:114	water sorption/diffusion	91:114	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	0	62	dep	oxide	9:13	arg1	wettability					117:127	wettability	117:127	wettability	117:127	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	2	63	theme	%	375:375	arg1	oxide					386:390	1% graphene oxide	374:390	1% graphene oxide (GO)	374:395	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30845148	0	64	theme	Antibacterial	39:51	arg1	activity					53:60	Antibacterial activity	39:60	Antibacterial activity	39:60	Graphene oxide in zinc alginate films: Antibacterial activity, cytotoxicity, zinc release, water sorption/diffusion, wettability and opacity.					
30845148	8	65	theme	types	1460:1464	arg1	use					1443:1445	the use	1439:1445	the use of different types of sodium alginates	1439:1484	However, neither the combination of GO with Zn2+ nor the use of different types of sodium alginates modified the antibacterial activity and cytotoxicity of the zinc alginates against these Gram-positive pathogens and human cells respectively.					
30845148	2	66	theme	1	374:374	arg1	%					375:375	%	375:375	%	375:375	We have recently demonstrated that the incorporation of 1% graphene oxide (GO) into alginate films crosslinked with Ca2+ cations provides antibacterial activity against Staphylococcus aureus and methicillin-resistant Staphylococcus epidermidis, and no cytotoxicity for human keratinocyte HaCaT cells.					
30452982	2	0	theme	initial	461:467	arg1	pH					469:470	initial pH	461:470	initial pH of the solution	461:486	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	2	0	theme	initial	461:467	arg1	NDIO					455:458	NDIO	455:458	NDIO	455:458	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	7	1	theme	exothermic	1444:1453	arg1	process					1471:1477	an exothermic and spontaneous process	1441:1477	an exothermic and spontaneous process	1441:1477	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	2	2	theme	initial	510:516	arg1	concentration					518:530	adsorbent, initial concentration	499:530	concentration	518:530	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	0	3	theme	adsorbent	67:75	arg1	chitosan/nanodiopside					77:97	a novel adsorbent chitosan/nanodiopside	59:97	a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm	59:180	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	6	4	theme	isotherm	1174:1181	arg1	model					1183:1187	the Langmuir monolayer isotherm model	1151:1187	the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937)	1151:1224	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	1	5	theme	novel	185:189	arg1	CS-NDIO					241:247	CS-NDIO	241:247	CS-NDIO	241:247	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	1	5	theme	novel	185:189	arg1	nanocomposite					226:238	A novel adsorbent of chitosan/nanodiopside nanocomposite	183:238	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO)	183:248	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	1	5	theme	novel	185:189	arg1	composite					277:285	a green composite	269:285	a green composite for the removal of crystal violet (CV)	269:324	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	2	6	dep	CCD	615:617	arg1	levels					622:627	5 levels	620:627	5 levels	620:627	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	2	6	dep	CCD	615:617	arg1	factors					635:641	4 factors	633:641	4 factors	633:641	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	5	7	theme	adsorption	869:878	arg1	process					880:886	maximum CV adsorption process	858:886	maximum CV adsorption process using CCD and ANN-GA	858:907	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	0	8	theme	response	105:112	arg1	methodology					122:132	response surface methodology	105:132	response surface methodology	105:132	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	0	9	from	Decolorization	0:13	arg1	solutions					46:54	aqueous solutions	38:54	aqueous solutions	38:54	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	2	10	theme	time	550:553	arg1	concentration					518:530	adsorbent, initial concentration	499:530	concentration	518:530	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	3	11	dep	data	752:755	arg1	get					757:759	get	757:759	get through CCD	757:771	Also, Hybrid model of (ANN) model with genetic algorithm (GA) optimization was applied to the experimental data get through CCD.					
30452982	3	12	theme	Hybrid	651:656	arg1	model					658:662	Hybrid model	651:662	Hybrid model of (ANN) model with genetic algorithm (GA) optimization	651:718	Also, Hybrid model of (ANN) model with genetic algorithm (GA) optimization was applied to the experimental data get through CCD.					
30452982	2	13	theme	contact	542:548	arg1	time					550:553	contact time	542:553	contact time	542:553	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	5	14	theme	maximum	858:864	arg1	process					880:886	maximum CV adsorption process	858:886	maximum CV adsorption process using CCD and ANN-GA	858:907	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	2	15	theme	solution	479:486	arg1	pH					469:470	initial pH	461:470	initial pH of the solution	461:486	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	2	15	theme	solution	479:486	arg1	NDIO					455:458	NDIO	455:458	NDIO	455:458	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	0	16	from	solutions	46:54	arg1	Decolorization					0:13	Decolorization	0:13	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.	0:181	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	0	16	from	solutions	46:54	arg1	violet					26:31	crystal violet	18:31	crystal violet from aqueous solutions	18:54	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	2	17	theme	central	589:595	arg1	design					607:612	central composite design	589:612	central composite design (CCD; 5 levels and 4 factors)	589:642	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	2	18	theme	composite	597:605	arg1	design					607:612	central composite design	589:612	central composite design (CCD; 5 levels and 4 factors)	589:642	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	5	19	theme	CV	866:867	arg1	process					880:886	maximum CV adsorption process	858:886	maximum CV adsorption process using CCD and ANN-GA	858:907	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	7	20	dep	parameters	1297:1306	arg1	R2					1309:1310	R2	1309:1310	R2	1309:1310	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	7	20	dep	parameters	1297:1306	arg1	ΔH°					1321:1323	ΔH°	1321:1323	ΔH°	1321:1323	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	1	21	theme	adsorbent	191:199	arg1	CS-NDIO					241:247	CS-NDIO	241:247	CS-NDIO	241:247	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	1	21	theme	adsorbent	191:199	arg1	nanocomposite					226:238	A novel adsorbent of chitosan/nanodiopside nanocomposite	183:238	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO)	183:248	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	1	21	theme	adsorbent	191:199	arg1	composite					277:285	a green composite	269:285	a green composite for the removal of crystal violet (CV)	269:324	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	0	22	theme	surface	114:120	arg1	methodology					122:132	response surface methodology	105:132	response surface methodology	105:132	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	6	23	dep	qmax	1190:1193	arg1	0.9937					1218:1223	0.9937	1218:1223	0.9937	1218:1223	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	6	23	dep	qmax	1190:1193	arg1	R2					1214:1215	R2	1214:1215	R2	1214:1215	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	6	23	dep	qmax	1190:1193	arg1	104.66 mg g-1					1196:1208	104.66 mg g-1	1196:1208	104.66 mg g-1	1196:1208	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	6	24	theme	kinetic	1121:1127	arg1	data					1129:1132	kinetic data	1121:1132	kinetic data	1121:1132	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	7	25	theme	spontaneous	1459:1469	arg1	process					1471:1477	an exothermic and spontaneous process	1441:1477	an exothermic and spontaneous process	1441:1477	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	2	26	theme	molar	433:437	arg1	ratios					439:444	molar ratios	433:444	molar ratios of CS	433:450	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	6	27	theme	order	1244:1248	arg1	kinetics					1250:1257	pseudo-second order kinetics	1230:1257	pseudo-second order kinetics mechanism (R2: 0.9978)	1230:1280	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	4	28	theme	molar	788:792	arg1	ratio					794:798	The optimized molar ratio	774:798	The optimized molar ratio of CS-NDIO	774:809	The optimized molar ratio of CS-NDIO was found: 20/80.					
30452982	1	29	theme	chitosan/nanodiopside	204:224	arg1	adsorbent					191:199	adsorbent	191:199	adsorbent	191:199	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	2	30	theme	adsorbent	499:507	arg1	concentration					518:530	adsorbent, initial concentration	499:530	concentration	518:530	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	5	31	theme	contact	1030:1036	arg1	time					1038:1041	contact time	1030:1041	contact time	1030:1041	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	31	theme	contact	1030:1036	arg1	0.0077					963:968	0.0077	963:968	0.0077	963:968	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	6	32	dep	R2	1270:1271	arg1	0.9978					1274:1279	0.9978	1274:1279	R2: 0.9978	1270:1279	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	5	33	theme	Optimal	829:835	arg1	choice					847:852	Optimal parameter choice	829:852	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA	829:907	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	34	dep	were	909:912	arg1	7.499					940:944	7.499	940:944	7.499	940:944	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	34	dep	were	909:912	arg1	mass					957:960	adsorbent mass	947:960	adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min	947:1062	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	34	dep	were	909:912	arg1	pH = 7.50					926:934	pH = 7.50	926:934	pH = 7.50	926:934	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	0	35	theme	violet	26:31	arg1	Decolorization					0:13	Decolorization	0:13	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.	0:181	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	1	36	theme	crystal	306:312	arg1	violet					314:319	crystal violet	306:319	crystal violet (CV)	306:324	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	1	36	theme	crystal	306:312	arg1	CV					322:323	CV	322:323	CV	322:323	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	0	37	theme	artificial	138:147	arg1	algorithm					172:180	artificial neural network-genetic algorithm	138:180	artificial neural network-genetic algorithm	138:180	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	5	38	theme	parameter	837:845	arg1	choice					847:852	Optimal parameter choice	829:852	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA	829:907	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	2	39	theme	parameters	417:426	arg1	influence					404:412	The influence	400:412	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time	400:553	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	1	40	theme	violet	314:319	arg1	removal					295:301	the removal	291:301	the removal of crystal violet (CV)	291:324	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	6	41	dep	kinetics	1250:1257	arg1	R2					1270:1271	R2	1270:1271	R2: 0.9978	1270:1279	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	6	41	dep	kinetics	1250:1257	arg1	mechanism					1259:1267	mechanism	1259:1267	pseudo-second order kinetics mechanism (R2: 0.9978)	1230:1280	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	0	42	theme	aqueous	38:44	arg1	solutions					46:54	aqueous solutions	38:54	aqueous solutions	38:54	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	0	43	theme	network-genetic	156:170	arg1	algorithm					172:180	artificial neural network-genetic algorithm	138:180	artificial neural network-genetic algorithm	138:180	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	5	44	theme	adsorbent	947:955	arg1	mass					957:960	adsorbent mass	947:960	adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min	947:1062	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	44	theme	adsorbent	947:955	arg1	pH = 7.50					926:934	pH = 7.50	926:934	pH = 7.50	926:934	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	6	45	theme	adsorption	1094:1103	arg1	equilibrium					1105:1115	The evaluation adsorption equilibrium	1079:1115	The evaluation adsorption equilibrium	1079:1115	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	3	46	theme	genetic	684:690	arg1	GA					703:704	GA	703:704	GA	703:704	Also, Hybrid model of (ANN) model with genetic algorithm (GA) optimization was applied to the experimental data get through CCD.					
30452982	3	46	theme	genetic	684:690	arg1	algorithm					692:700	genetic algorithm	684:700	genetic algorithm (GA) optimization	684:718	Also, Hybrid model of (ANN) model with genetic algorithm (GA) optimization was applied to the experimental data get through CCD.					
30452982	0	47	theme	neural	149:154	arg1	algorithm					172:180	artificial neural network-genetic algorithm	138:180	artificial neural network-genetic algorithm	138:180	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	1	48	theme	FESEM	384:388	arg1	analysis					390:397	FESEM analysis	384:397	FESEM analysis	384:397	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	6	49	theme	evaluation	1083:1092	arg1	equilibrium					1105:1115	The evaluation adsorption equilibrium	1079:1115	The evaluation adsorption equilibrium	1079:1115	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	6	50	theme	Langmuir	1155:1162	arg1	model					1183:1187	the Langmuir monolayer isotherm model	1151:1187	the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937)	1151:1224	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	2	51	theme	CS	449:450	arg1	ratios					439:444	molar ratios	433:444	molar ratios of CS	433:450	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	3	52	theme	algorithm	692:700	arg1	optimization					707:718	genetic algorithm (GA) optimization	684:718	genetic algorithm (GA) optimization	684:718	Also, Hybrid model of (ANN) model with genetic algorithm (GA) optimization was applied to the experimental data get through CCD.					
30452982	6	53	dep	model	1183:1187	arg1	qmax					1190:1193	qmax	1190:1193	qmax: 104.66 mg g-1 and R2: 0.9937	1190:1223	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	6	54	theme	monolayer	1164:1172	arg1	model					1183:1187	the Langmuir monolayer isotherm model	1151:1187	the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937)	1151:1224	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	3	55	theme	model	673:677	arg1	model					658:662	Hybrid model	651:662	Hybrid model of (ANN) model with genetic algorithm (GA) optimization	651:718	Also, Hybrid model of (ANN) model with genetic algorithm (GA) optimization was applied to the experimental data get through CCD.					
30452982	2	56	theme	CV	535:536	arg1	concentration					518:530	adsorbent, initial concentration	499:530	concentration	518:530	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	4	57	theme	optimized	778:786	arg1	ratio					794:798	The optimized molar ratio	774:798	The optimized molar ratio of CS-NDIO	774:809	The optimized molar ratio of CS-NDIO was found: 20/80.					
30452982	7	58	dep	R2	1309:1310	arg1	-12.89 kJ mol-1					1348:1362	-12.89 kJ mol-1	1348:1362	-12.89 kJ mol-1	1348:1362	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	7	58	dep	R2	1309:1310	arg1	-74.93 kJ mol-1					1326:1340	-74.93 kJ mol-1	1326:1340	-74.93 kJ mol-1	1326:1340	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	7	58	dep	R2	1309:1310	arg1	ΔS°					1369:1371	ΔS°	1369:1371	ΔS°	1369:1371	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	7	58	dep	R2	1309:1310	arg1	ΔG°					1343:1345	ΔG°	1343:1345	ΔG°	1343:1345	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	7	58	dep	R2	1309:1310	arg1	0.93 kJ mol-1 K-1					1374:1390	0.93 kJ mol-1 K-1	1374:1390	0.93 kJ mol-1 K-1	1374:1390	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	7	58	dep	R2	1309:1310	arg1	0.9180					1313:1318	0.9180	1313:1318	0.9180	1313:1318	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	4	59	theme	CS-NDIO	803:809	arg1	ratio					794:798	The optimized molar ratio	774:798	The optimized molar ratio of CS-NDIO	774:809	The optimized molar ratio of CS-NDIO was found: 20/80.					
30452982	7	60	theme	Thermodynamic	1283:1295	arg1	parameters					1297:1306	Thermodynamic parameters	1283:1306	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1)	1283:1391	Thermodynamic parameters (R2: 0.9180, ΔH°: -74.93 kJ mol-1, ΔG°: -12.89 kJ mol-1, and ΔS°: 0.93 kJ mol-1 K-1) were calculated and indicating adsorption to be an exothermic and spontaneous process.					
30452982	5	61	dep	mass	957:960	arg1	concentration					987:999	CV concentration	984:999	CV concentration	984:999	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	61	dep	mass	957:960	arg1	20.000					1002:1007	20.000	1002:1007	20.000	1002:1007	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	61	dep	mass	957:960	arg1	time					1038:1041	contact time	1030:1041	contact time	1030:1041	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	61	dep	mass	957:960	arg1	0.0077					963:968	0.0077	963:968	0.0077	963:968	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	61	dep	mass	957:960	arg1	25.00					1044:1048	25.00	1044:1048	25.00	1044:1048	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	61	dep	mass	957:960	arg1	0.0077 g					974:981	0.0077 g	974:981	0.0077 g	974:981	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	61	dep	mass	957:960	arg1	25.00 min					1054:1062	25.00 min	1054:1062	25.00 min	1054:1062	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	2	62	theme	concentration	518:530	arg1	NDIO					455:458	NDIO	455:458	NDIO	455:458	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	2	62	theme	concentration	518:530	arg1	dosage					489:494	dosage	489:494	dosage of adsorbent, initial concentration of CV and contact time	489:553	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	3	63	theme	experimental	739:750	arg1	data					752:755	the experimental data	735:755	the experimental data get through CCD	735:771	Also, Hybrid model of (ANN) model with genetic algorithm (GA) optimization was applied to the experimental data get through CCD.					
30452982	0	64	theme	novel	61:65	arg1	chitosan/nanodiopside					77:97	a novel adsorbent chitosan/nanodiopside	59:97	a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm	59:180	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	3	65	with	model	658:662	arg1	optimization					707:718	genetic algorithm (GA) optimization	684:718	genetic algorithm (GA) optimization	684:718	Also, Hybrid model of (ANN) model with genetic algorithm (GA) optimization was applied to the experimental data get through CCD.					
30452982	6	66	theme	pseudo-second	1230:1242	arg1	kinetics					1250:1257	pseudo-second order kinetics	1230:1257	pseudo-second order kinetics mechanism (R2: 0.9978)	1230:1280	The evaluation adsorption equilibrium and kinetic data were fitted with the Langmuir monolayer isotherm model (qmax: 104.66 mg g-1 and R2: 0.9937) and pseudo-second order kinetics mechanism (R2: 0.9978).					
30452982	5	67	theme	CV	984:985	arg1	concentration					987:999	CV concentration	984:999	CV concentration	984:999	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	5	67	theme	CV	984:985	arg1	0.0077					963:968	0.0077	963:968	0.0077	963:968	Optimal parameter choice for maximum CV adsorption process using CCD and ANN-GA were as follows: pH = 7.50 and 7.499, adsorbent mass: 0.0077 and 0.0077 g, CV concentration: 20.000 and 20.002 mg/L, and contact time: 25.00 and 25.00 min, respectively.					
30452982	0	68	theme	crystal	18:24	arg1	violet					26:31	crystal violet	18:31	crystal violet from aqueous solutions	18:54	Decolorization of crystal violet from aqueous solutions by a novel adsorbent chitosan/nanodiopside using response surface methodology and artificial neural network-genetic algorithm.					
30452982	2	69	dep	design	607:612	arg1	CCD					615:617	CCD	615:617	CCD; 5 levels and 4 factors	615:641	The influence of parameters like molar ratios of CS to NDIO, initial pH of the solution, dosage of adsorbent, initial concentration of CV and contact time was investigated and evaluated by central composite design (CCD; 5 levels and 4 factors).					
30452982	1	70	theme	green	271:275	arg1	nanocomposite					226:238	A novel adsorbent of chitosan/nanodiopside nanocomposite	183:238	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO)	183:248	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30452982	1	70	theme	green	271:275	arg1	composite					277:285	a green composite	269:285	a green composite for the removal of crystal violet (CV)	269:324	A novel adsorbent of chitosan/nanodiopside nanocomposite (CS-NDIO) was synthesized as a green composite for the removal of crystal violet (CV) and characterized by techniques like XRD, FT-IR, BET, and FESEM analysis.					
30903674	6	0	theme	liver	1407:1411	arg1	data					1427:1430	the previous liver transcriptome data	1394:1430	the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle	1394:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	6	1	from	fat	1617:1619	arg1	cattle					1662:1667	dairy cattle	1656:1667	dairy cattle	1656:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	4	2	theme	milk	1014:1017	arg1	synthesis					1019:1027	milk synthesis	1014:1027	milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways	1014:1130	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	4	3	theme	pathway	887:893	arg1	analysis					906:913	KEGG pathway functional analysis	882:913	KEGG pathway functional analysis	882:913	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	6	4	theme	same	1446:1449	arg1	samples					1451:1457	the same samples	1442:1457	the same samples	1442:1457	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	0	5	theme	Milk	77:80	arg1	Fat					82:84	Milk Fat	77:84	Milk Fat	77:84	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	1	6	theme	relative	293:300	arg1	quantification					315:328	relative and absolute quantification	293:328	relative and absolute quantification (iTRAQ)	293:336	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	1	6	theme	relative	293:300	arg1	iTRAQ					331:335	iTRAQ	331:335	iTRAQ	331:335	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	5	7	theme	milk	1316:1319	arg1	yield					1321:1325	milk yield	1316:1325	milk yield	1316:1325	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	1	8	theme	functional	386:395	arg1	proteins/genes					397:410	functional proteins/genes	386:410	functional proteins/genes involved in milk synthesis in dairy cattle	386:453	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	4	9	theme	signaling	1113:1121	arg1	pathways					1123:1130	PPAR signaling pathways	1108:1130	PPAR signaling pathways	1108:1130	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	2	10	theme	3252	481:484	arg1	proteins					486:493	3252 proteins	481:493	3252 proteins	481:493	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	2	11	theme	discovery	536:544	arg1	rate					546:549	false discovery rate ≤0.01	530:555	false discovery rate ≤0.01	530:555	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	2	11	theme	discovery	536:544	arg1	tissues					521:527	the liver tissues	511:527	the liver tissues (false discovery rate ≤0.01)	511:556	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	4	12	theme	starch	1064:1069	arg1	metabolism					1083:1092	starch and sucrose metabolism	1064:1092	metabolism	1083:1092	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	0	13	with	Cattle	128:133	arg1	iTRAQ					140:144	iTRAQ	140:144	iTRAQ	140:144	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	6	14	theme	promising	1587:1595	arg1	candidates					1597:1606	the promising candidates	1583:1606	the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle	1583:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	1	15	theme	absolute	306:313	arg1	quantification					315:328	relative and absolute quantification	293:328	relative and absolute quantification (iTRAQ)	293:336	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	1	15	theme	absolute	306:313	arg1	iTRAQ					331:335	iTRAQ	331:335	iTRAQ	331:335	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	2	16	theme	false	530:534	arg1	rate					546:549	false discovery rate ≤0.01	530:555	false discovery rate ≤0.01	530:555	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	2	16	theme	false	530:534	arg1	tissues					521:527	the liver tissues	511:527	the liver tissues (false discovery rate ≤0.01)	511:556	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	5	17	theme	significant	1252:1262	arg1	polymorphisms					1282:1294	significant single nucleotide polymorphisms	1252:1294	significant single nucleotide polymorphisms that associate with milk yield and composition traits	1252:1348	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	3	18	theme	p-value	720:726	arg1	<0.05					728:732	p-value <0.05	720:732	p-value <0.05	720:732	Thirty-two differently expressed proteins (DEPs) are identified during the three periods by p-value <0.05 and fold change (FC) ≥2 or ≤0.5, and 183 DEPs based on p-value <0.05 and FC ≥1.5 or ≤0.67.					
30903674	3	19	theme	fold	669:672	arg1	FC					682:683	FC	682:683	FC	682:683	Thirty-two differently expressed proteins (DEPs) are identified during the three periods by p-value <0.05 and fold change (FC) ≥2 or ≤0.5, and 183 DEPs based on p-value <0.05 and FC ≥1.5 or ≤0.67.					
30903674	3	19	theme	fold	669:672	arg1	change					674:679	fold change	669:679	fold change (FC)	669:684	Thirty-two differently expressed proteins (DEPs) are identified during the three periods by p-value <0.05 and fold change (FC) ≥2 or ≤0.5, and 183 DEPs based on p-value <0.05 and FC ≥1.5 or ≤0.67.					
30903674	5	20	theme	nucleotide	1271:1280	arg1	polymorphisms					1282:1294	significant single nucleotide polymorphisms	1252:1294	significant single nucleotide polymorphisms that associate with milk yield and composition traits	1252:1348	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	4	21	theme	sucrose	1075:1081	arg1	metabolism					1083:1092	starch and sucrose metabolism	1064:1092	metabolism	1083:1092	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	4	22	theme	metabolic	973:981	arg1	terms					983:987	metabolic terms	973:987	metabolic terms	973:987	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	2	23	theme	iTRAQ	465:469	arg1	analysis					471:478	iTRAQ analysis	465:478	iTRAQ analysis	465:478	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	3	24	theme	p-value	651:657	arg1	<0.05					659:663	p-value <0.05	651:663	p-value <0.05	651:663	Thirty-two differently expressed proteins (DEPs) are identified during the three periods by p-value <0.05 and fold change (FC) ≥2 or ≤0.5, and 183 DEPs based on p-value <0.05 and FC ≥1.5 or ≤0.67.					
30903674	7	25	theme	critical	1733:1740	arg1	genes					1742:1746	the potential critical genes	1719:1746	the potential critical genes associated with milk synthesis of dairy cattle	1719:1793	This study provides a new vision for identifying the potential critical genes associated with milk synthesis of dairy cattle.					
30903674	1	26	theme	milk	424:427	arg1	synthesis					429:437	milk synthesis	424:437	milk synthesis in dairy cattle	424:453	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	7	27	theme	milk	1764:1767	arg1	synthesis					1769:1777	milk synthesis	1764:1777	milk synthesis of dairy cattle	1764:1793	This study provides a new vision for identifying the potential critical genes associated with milk synthesis of dairy cattle.					
30903674	6	28	from	synthesis	1643:1651	arg1	cattle					1662:1667	dairy cattle	1656:1667	dairy cattle	1656:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	4	29	dep	GO	875:876	arg1	the					860:862	the	860:862	the	860:862	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	5	30	theme	reported	1214:1221	arg1	locus					1242:1246	the reported quantitative trait locus	1210:1246	the reported quantitative trait locus	1210:1246	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	4	31	theme	p-value	819:825	arg1	<0.05					827:831	p-value <0.05	819:831	p-value <0.05	819:831	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	7	32	theme	new	1692:1694	arg1	vision					1696:1701	a new vision	1690:1701	a new vision for identifying the potential critical genes associated with milk synthesis of dairy cattle	1690:1793	This study provides a new vision for identifying the potential critical genes associated with milk synthesis of dairy cattle.					
30903674	6	33	used	used	1437:1440	arg2	data					1427:1430	the previous liver transcriptome data	1394:1430	the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle	1394:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	1	34	theme	lactation	234:242	arg1	cycle					244:248	the lactation cycle	230:248	the lactation cycle of Holstein cows	230:265	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	5	35	theme	quantitative	1223:1234	arg1	locus					1242:1246	the reported quantitative trait locus	1210:1246	the reported quantitative trait locus	1210:1246	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	2	36	located	detected	499:506	arg1	rate					546:549	false discovery rate ≤0.01	530:555	false discovery rate ≤0.01	530:555	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	2	36	located	detected	499:506	arg2	proteins					486:493	3252 proteins	481:493	3252 proteins	481:493	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	2	36	located	detected	499:506	arg1	tissues					521:527	the liver tissues	511:527	the liver tissues (false discovery rate ≤0.01)	511:556	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	6	37	theme	dairy	1656:1660	arg1	cattle					1662:1667	dairy cattle	1656:1667	dairy cattle	1656:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	0	38	theme	Proteome	18:25	arg1	Analysis					0:7	Analysis	0:7	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.	0:145	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	1	39	theme	cycle	244:248	arg1	periods					219:225	three periods	213:225	three periods of the lactation cycle of Holstein cows	213:265	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	1	40	theme	dairy	442:446	arg1	cattle					448:453	dairy cattle	442:453	dairy cattle	442:453	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	5	41	theme	locus	1242:1246	arg1	locations					1197:1205	the peak locations	1188:1205	the peak locations of the reported quantitative trait locus	1188:1246	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	5	41	theme	locus	1242:1246	arg1	polymorphisms					1282:1294	significant single nucleotide polymorphisms	1252:1294	significant single nucleotide polymorphisms that associate with milk yield and composition traits	1252:1348	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	0	42	theme	Liver	12:16	arg1	Proteome					18:25	Liver Proteome	12:25	Liver Proteome	12:25	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	6	43	dep	considered	1478:1487	arg1	candidates					1597:1606	the promising candidates	1583:1606	the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle	1583:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	0	44	theme	Lactose	100:106	arg1	Metabolism					108:117	Lactose Metabolism	100:117	Lactose Metabolism	100:117	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	0	45	from	Fat	82:84	arg1	Cattle					128:133	Dariy Cattle	122:133	Dariy Cattle with iTRAQ	122:144	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	6	46	theme	lactose	1635:1641	arg1	synthesis					1643:1651	lactose synthesis	1635:1651	lactose synthesis	1635:1651	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	0	47	theme	Identification	31:44	arg1	Analysis					0:7	Analysis	0:7	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.	0:145	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	1	48	theme	Holstein	253:260	arg1	cows					262:265	Holstein cows	253:265	Holstein cows	253:265	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	0	49	theme	Dariy	122:126	arg1	Cattle					128:133	Dariy Cattle	122:133	Dariy Cattle with iTRAQ	122:144	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	4	50	theme	PPAR	1108:1111	arg1	pathways					1123:1130	PPAR signaling pathways	1108:1130	PPAR signaling pathways	1108:1130	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	1	51	theme	cows	262:265	arg1	cycle					244:248	the lactation cycle	230:248	the lactation cycle of Holstein cows	230:265	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	3	52	theme	expressed	582:590	arg1	DEPs					602:605	DEPs	602:605	DEPs	602:605	Thirty-two differently expressed proteins (DEPs) are identified during the three periods by p-value <0.05 and fold change (FC) ≥2 or ≤0.5, and 183 DEPs based on p-value <0.05 and FC ≥1.5 or ≤0.67.					
30903674	3	52	theme	expressed	582:590	arg1	proteins					592:599	Thirty-two differently expressed proteins	559:599	Thirty-two differently expressed proteins (DEPs)	559:606	Thirty-two differently expressed proteins (DEPs) are identified during the three periods by p-value <0.05 and fold change (FC) ≥2 or ≤0.5, and 183 DEPs based on p-value <0.05 and FC ≥1.5 or ≤0.67.					
30903674	4	53	theme	KEGG	882:885	arg1	analysis					906:913	KEGG pathway functional analysis	882:913	KEGG pathway functional analysis	882:913	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	6	54	theme	transcriptome	1413:1425	arg1	data					1427:1430	the previous liver transcriptome data	1394:1430	the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle	1394:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	1	55	theme	liver	179:183	arg1	tissues					185:191	liver tissues	179:191	liver tissues	179:191	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	0	56	theme	Critical	49:56	arg1	Proteins					58:65	Critical Proteins	49:65	Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ	49:144	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	3	57	dep	<0.05	659:663	arg1	DEPs					706:709	≥2 or ≤0.5, and 183 DEPs	686:709	≥2 or ≤0.5, and 183 DEPs based on p-value <0.05 and FC ≥1.5 or ≤0.67	686:753	Thirty-two differently expressed proteins (DEPs) are identified during the three periods by p-value <0.05 and fold change (FC) ≥2 or ≤0.5, and 183 DEPs based on p-value <0.05 and FC ≥1.5 or ≤0.67.					
30903674	5	58	theme	composition	1331:1341	arg1	traits					1343:1348	composition traits	1331:1348	composition traits	1331:1348	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	4	59	theme	FC	837:838	arg1	≥1.2					840:843	FC ≥1.2	837:843	FC ≥1.2	837:843	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	5	60	theme	peak	1192:1195	arg1	locations					1197:1205	the peak locations	1188:1205	the peak locations of the reported quantitative trait locus	1188:1246	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	7	61	theme	potential	1723:1731	arg1	genes					1742:1746	the potential critical genes	1719:1746	the potential critical genes associated with milk synthesis of dairy cattle	1719:1793	This study provides a new vision for identifying the potential critical genes associated with milk synthesis of dairy cattle.					
30903674	7	62	theme	dairy	1782:1786	arg1	cattle					1788:1793	dairy cattle	1782:1793	dairy cattle	1782:1793	This study provides a new vision for identifying the potential critical genes associated with milk synthesis of dairy cattle.					
30903674	1	63	theme	liver	358:362	arg1	proteome					364:371	liver proteome	358:371	liver proteome	358:371	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	6	64	theme	previous	1398:1405	arg1	data					1427:1430	the previous liver transcriptome data	1394:1430	the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle	1394:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	6	65	from	protein	1622:1628	arg1	cattle					1662:1667	dairy cattle	1656:1667	dairy cattle	1656:1667	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30903674	1	66	theme	isobaric	276:283	arg1	tag					285:287	isobaric tag	276:287	isobaric tag for relative and absolute quantification (iTRAQ) technique	276:346	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	2	67	theme	liver	515:519	arg1	rate					546:549	false discovery rate ≤0.01	530:555	false discovery rate ≤0.01	530:555	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	2	67	theme	liver	515:519	arg1	tissues					521:527	the liver tissues	511:527	the liver tissues (false discovery rate ≤0.01)	511:556	Based on iTRAQ analysis, 3252 proteins are detected in the liver tissues (false discovery rate ≤0.01).					
30903674	4	68	theme	fatty	1052:1056	arg1	acid					1058:1061	fatty acid	1052:1061	fatty acid	1052:1061	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	4	69	theme	citrate	1037:1043	arg1	cycle					1045:1049	citrate cycle	1037:1049	citrate cycle	1037:1049	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	1	70	theme	tissues	185:191	arg1	proteomes					166:174	the proteomes	162:174	the proteomes of liver tissues	162:191	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	0	71	from	Protein	87:93	arg1	Cattle					128:133	Dariy Cattle	122:133	Dariy Cattle with iTRAQ	122:144	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	1	72	from	synthesis	429:437	arg1	cattle					448:453	dairy cattle	442:453	dairy cattle	442:453	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	5	73	theme	single	1264:1269	arg1	polymorphisms					1282:1294	significant single nucleotide polymorphisms	1252:1294	significant single nucleotide polymorphisms that associate with milk yield and composition traits	1252:1348	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	5	74	theme	trait	1236:1240	arg1	locus					1242:1246	the reported quantitative trait locus	1210:1246	the reported quantitative trait locus	1210:1246	Further, 41 out of 73 DEPs are identified near to both the peak locations of the reported quantitative trait locus and significant single nucleotide polymorphisms that associate with milk yield and composition traits.					
30903674	0	75	from	Metabolism	108:117	arg1	Cattle					128:133	Dariy Cattle	122:133	Dariy Cattle with iTRAQ	122:144	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	0	76	theme	Proteins	58:65	arg1	Identification					31:44	Identification	31:44	Identification	31:44	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	0	76	theme	Proteins	58:65	arg1	Proteome					18:25	Liver Proteome	12:25	Liver Proteome	12:25	Analysis of Liver Proteome and Identification of Critical Proteins Affecting Milk Fat, Protein, and Lactose Metabolism in Dariy Cattle with iTRAQ.					
30903674	1	77	theme	tag	285:287	arg1	technique					338:346	isobaric tag for relative and absolute quantification (iTRAQ) technique	276:346	isobaric tag for relative and absolute quantification (iTRAQ) technique	276:346	In this study, the proteomes of liver tissues are investigated in three periods of the lactation cycle of Holstein cows by using isobaric tag for relative and absolute quantification (iTRAQ) technique to obtain liver proteome and identify functional proteins/genes involved in milk synthesis in dairy cattle.					
30903674	7	78	theme	cattle	1788:1793	arg1	synthesis					1769:1777	milk synthesis	1764:1777	milk synthesis of dairy cattle	1764:1793	This study provides a new vision for identifying the potential critical genes associated with milk synthesis of dairy cattle.					
30903674	4	79	dep	terms	983:987	arg1	the					969:971	the	969:971	the	969:971	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	4	80	theme	functional	895:904	arg1	analysis					906:913	KEGG pathway functional analysis	882:913	KEGG pathway functional analysis	882:913	In addition, 905 DEPs are obtained across the three periods by p-value <0.05 and FC ≥1.2, or ≤0.83, and the subsequent GO and KEGG pathway functional analysis indicate that 73 DEPs are significantly enriched into the metabolic terms and pathways involved in milk synthesis such as citrate cycle, fatty acid, starch and sucrose metabolism, and mTOR and PPAR signaling pathways.					
30903674	6	81	theme	milk	1612:1615	arg1	fat					1617:1619	milk fat	1612:1619	milk fat	1612:1619	In addition, the 41 DEPs are analyzed with the previous liver transcriptome data that used the same samples as this study, and considered nine proteins/genes-ALDH18A1, APOA4, CYP7A1, HADHB, PRKACA, IDH2, LDHA, LDHB, and MAT2A-to be the promising candidates for milk fat, protein, and lactose synthesis in dairy cattle.					
30270846	4	0	theme	3D	665:666	arg1	hydrogel					668:675	a 3D hydrogel	663:675	a 3D hydrogel	663:675	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	4	0	theme	3D	665:666	arg1	type					588:591	Collagen type I	579:593	Collagen type I	579:593	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	2	1	theme	in	345:346	arg1	microenvironment					360:375	the in vivo tissue microenvironment	341:375	the in vivo tissue microenvironment	341:375	These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue microenvironment.					
30270846	9	2	theme	support	1796:1802	arg1	hydrogel					1804:1811	a support hydrogel	1794:1811	a support hydrogel for hepatocytes which were able to remain viable over two weeks and responded to drug treatment appropriately	1794:1921	Further, the bioink adequately allowed for implementation as a support hydrogel for hepatocytes which were able to remain viable over two weeks and responded to drug treatment appropriately.					
30270846	0	3	theme	liver	75:79	arg1	microenvironments					81:97	3D bioprinted liver microenvironments	61:97	3D bioprinted liver microenvironments	61:97	Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments.					
30270846	5	4	with	bioink	850:855	arg1	properties					866:875	the properties	862:875	the properties required for extrusion bioprinting	862:910	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	4	5	theme	slow	745:748	arg1	polymerization					750:763	slow polymerization	745:763	slow polymerization	745:763	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	8	6	theme	hyaluronic	1603:1612	arg1	acid					1614:1617	thiolated hyaluronic acid	1593:1617	thiolated hyaluronic acid	1593:1617	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	2	7	dep	in	345:346	arg1	vivo					348:351	vivo	348:351	vivo	348:351	These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue microenvironment.					
30270846	8	8	theme	simple	1627:1632	arg1	bioink					1645:1650	a simple, printable bioink	1625:1650	a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation	1625:1730	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	6	9	theme	collagen	1133:1140	arg1	type					1142:1145	4:1 collagen type I	1129:1147	4:1 collagen type I to hyaluronic acid	1129:1166	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	6	10	theme	4:1	1129:1131	arg1	type					1142:1145	4:1 collagen type I	1129:1147	4:1 collagen type I to hyaluronic acid	1129:1166	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	5	11	theme	cell-matrix	937:947	arg1	interactions					949:960	native cell-matrix interactions	930:960	native cell-matrix interactions	930:960	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	3	12	theme	drug	548:551	arg1	metabolism					553:562	drug metabolism	548:562	drug metabolism	548:562	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	8	13	theme	collagen	1573:1580	arg1	type					1582:1585	methacrylated collagen type I	1559:1587	methacrylated collagen type I	1559:1587	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	3	14	theme	tissues	489:495	arg1	function					462:469	function	462:469	function	462:469	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	3	14	theme	tissues	489:495	arg1	phenotype					448:456	cellular phenotype	439:456	cellular phenotype	439:456	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	6	15	theme	optimal	1225:1231	arg1	formulation					1233:1243	an optimal formulation	1222:1243	an optimal formulation that allows for bioprinting while supporting biological activity	1222:1308	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	6	16	theme	viscoelastic	1061:1072	arg1	properties					1074:1083	the viscoelastic properties	1057:1083	the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid	1057:1166	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	7	17	theme	3D	1357:1358	arg1	constructs					1373:1382	bioprint 3D liver tissue constructs	1348:1382	bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells	1348:1444	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	1	18	theme	biomaterials	158:169	arg1	use					151:153	the use	147:153	the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM)	147:250	Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM).					
30270846	3	19	theme	disease	536:542	arg1	modeling					524:531	inaccurate modeling	513:531	inaccurate modeling of disease and drug metabolism	513:562	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	8	20	theme	stromal	1708:1714	arg1	elongation					1721:1730	stromal cell elongation	1708:1730	stromal cell elongation	1708:1730	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	7	21	theme	primary	1395:1401	arg1	hepatocytes					1409:1419	primary human hepatocytes	1395:1419	primary human hepatocytes	1395:1419	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	7	22	theme	tissue	1366:1371	arg1	constructs					1373:1382	bioprint 3D liver tissue constructs	1348:1382	bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells	1348:1444	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	4	23	theme	3D	705:706	arg1	bioprinting					708:718	3D bioprinting	705:718	3D bioprinting due to low viscosity and slow polymerization	705:763	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	8	24	theme	thiolated	1593:1601	arg1	acid					1614:1617	thiolated hyaluronic acid	1593:1617	thiolated hyaluronic acid	1593:1617	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	3	25	theme	biomaterials	418:429	arg1	nature					393:398	The artificial nature	378:398	The artificial nature of currently used biomaterials	378:429	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	3	26	theme	artificial	382:391	arg1	nature					393:398	The artificial nature	378:398	The artificial nature of currently used biomaterials	378:429	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	7	27	contain	containing	1384:1393	arg2	cells					1440:1444	liver stellate cells	1425:1444	liver stellate cells	1425:1444	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	7	27	contain	containing	1384:1393	arg1	constructs					1373:1382	bioprint 3D liver tissue constructs	1348:1382	bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells	1348:1444	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	7	27	contain	containing	1384:1393	arg2	hepatocytes					1409:1419	primary human hepatocytes	1395:1419	primary human hepatocytes	1395:1419	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	7	28	theme	common	1489:1494	arg1	toxicant					1502:1509	a common liver toxicant	1487:1509	a common liver toxicant	1487:1509	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	7	28	theme	common	1489:1494	arg1	acetaminophen					1472:1484	acetaminophen	1472:1484	acetaminophen	1472:1484	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	1	29	theme	Current	100:106	arg1	printing					111:118	Current 3D printing	100:118	Current 3D printing of tissue	100:128	Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM).					
30270846	8	30	theme	cell	1716:1719	arg1	elongation					1721:1730	stromal cell elongation	1708:1730	stromal cell elongation	1708:1730	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	2	31	theme	microenvironment	360:375	arg1	structure					328:336	structure	328:336	structure	328:336	These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue microenvironment.					
30270846	2	31	theme	microenvironment	360:375	arg1	composition					312:322	composition	312:322	composition	312:322	These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue microenvironment.					
30270846	0	32	theme	type	25:28	arg1	hybrid					43:48	collagen type I-hyaluronan hybrid	16:48	collagen type I-hyaluronan hybrid	16:48	Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments.					
30270846	7	33	theme	human	1403:1407	arg1	hepatocytes					1409:1419	primary human hepatocytes	1395:1419	primary human hepatocytes	1395:1419	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	5	34	theme	extrusion	890:898	arg1	bioprinting					900:910	extrusion bioprinting	890:910	extrusion bioprinting	890:910	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	1	35	theme	3D	108:109	arg1	printing					111:118	Current 3D printing	100:118	Current 3D printing of tissue	100:128	Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM).					
30270846	7	36	theme	liver	1360:1364	arg1	constructs					1373:1382	bioprint 3D liver tissue constructs	1348:1382	bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells	1348:1444	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	5	37	theme	native	930:935	arg1	interactions					949:960	native cell-matrix interactions	930:960	native cell-matrix interactions	930:960	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	0	38	theme	collagen	16:23	arg1	hybrid					43:48	collagen type I-hyaluronan hybrid	16:48	collagen type I-hyaluronan hybrid	16:48	Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments.					
30270846	4	39	theme	Collagen	579:586	arg1	hydrogel					668:675	a 3D hydrogel	663:675	a 3D hydrogel	663:675	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	4	39	theme	Collagen	579:586	arg1	type					588:591	Collagen type I	579:593	Collagen type I	579:593	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	4	39	theme	Collagen	579:586	arg1	component					619:627	the major structural component	598:627	the major structural component in the ECM	598:638	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	9	40	dep	bioink	1746:1751	arg1	allowed					1764:1770	allowed	1764:1770	bioink adequately allowed for implementation as a support hydrogel for hepatocytes which were able to remain viable over two weeks and responded to drug treatment appropriately	1746:1921	Further, the bioink adequately allowed for implementation as a support hydrogel for hepatocytes which were able to remain viable over two weeks and responded to drug treatment appropriately.					
30270846	6	41	theme	hyaluronic	1152:1161	arg1	acid					1163:1166	hyaluronic acid	1152:1166	hyaluronic acid	1152:1166	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	9	42	theme	drug	1894:1897	arg1	treatment					1899:1907	drug treatment	1894:1907	drug treatment	1894:1907	Further, the bioink adequately allowed for implementation as a support hydrogel for hepatocytes which were able to remain viable over two weeks and responded to drug treatment appropriately.					
30270846	2	43	theme	tissue	353:358	arg1	microenvironment					360:375	the in vivo tissue microenvironment	341:375	the in vivo tissue microenvironment	341:375	These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue microenvironment.					
30270846	0	44	theme	hybrid	43:48	arg1	Optimization					0:11	Optimization	0:11	Optimization of collagen type I-hyaluronan hybrid	0:48	Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments.					
30270846	2	45	theme	structure	328:336	arg1	optimization					296:307	optimization	296:307	optimization of composition and structure of the in vivo tissue microenvironment	296:375	These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue microenvironment.					
30270846	5	46	with	combination	794:804	arg1	collagen					830:837	collagen I	830:839	collagen I	830:839	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	5	47	theme	hyaluronic	809:818	arg1	acid					820:823	hyaluronic acid	809:823	hyaluronic acid	809:823	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	5	48	theme	microenvironment	993:1008	arg1	preservation					966:977	preservation	966:977	preservation of the native microenvironment	966:1008	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	5	48	theme	microenvironment	993:1008	arg1	interactions					949:960	native cell-matrix interactions	930:960	native cell-matrix interactions	930:960	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	1	49	theme	native	200:205	arg1	properties					207:216	the native properties	196:216	the native properties of the extracellular matrix (ECM)	196:250	Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM).					
30270846	0	50	theme	I-hyaluronan	30:41	arg1	hybrid					43:48	collagen type I-hyaluronan hybrid	16:48	collagen type I-hyaluronan hybrid	16:48	Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments.					
30270846	8	51	theme	methacrylated	1559:1571	arg1	type					1582:1585	methacrylated collagen type I	1559:1587	methacrylated collagen type I	1559:1587	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	3	52	theme	used	413:416	arg1	biomaterials					418:429	currently used biomaterials	403:429	currently used biomaterials	403:429	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	6	53	dep	formulations	1101:1112	arg1	-2:1					1114:1117	-2:1	1114:1117	-2:1	1114:1117	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	6	53	dep	formulations	1101:1112	arg1	type					1142:1145	4:1 collagen type I	1129:1147	4:1 collagen type I to hyaluronic acid	1129:1166	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	6	53	dep	formulations	1101:1112	arg1	3:1					1120:1122	3:1	1120:1122	3:1	1120:1122	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	4	54	used	used	655:658	arg2	type					588:591	Collagen type I	579:593	Collagen type I	579:593	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	4	54	used	used	655:658	arg2	component					619:627	the major structural component	598:627	the major structural component in the ECM	598:638	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	4	54	used	used	655:658	arg2	hydrogel					668:675	a 3D hydrogel	663:675	a 3D hydrogel	663:675	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	5	55	theme	acid	820:823	arg1	combination					794:804	a combination	792:804	a combination of hyaluronic acid with collagen I	792:839	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	6	56	theme	biological	1290:1299	arg1	activity					1301:1308	biological activity	1290:1308	biological activity	1290:1308	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	3	57	theme	cellular	439:446	arg1	phenotype					448:456	cellular phenotype	439:456	cellular phenotype	439:456	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	7	58	theme	bioprint	1348:1355	arg1	constructs					1373:1382	bioprint 3D liver tissue constructs	1348:1382	bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells	1348:1444	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	6	59	theme	bioink	1094:1099	arg1	formulations					1101:1112	three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid	1088:1166	three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid	1088:1166	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	5	60	dep	interactions	949:960	arg1	properties					1010:1019	properties	1010:1019	properties	1010:1019	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	8	61	theme	type	1582:1585	arg1	combination					1544:1554	the combination	1540:1554	the combination of methacrylated collagen type I	1540:1587	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	8	61	theme	type	1582:1585	arg1	acid					1614:1617	thiolated hyaluronic acid	1593:1617	thiolated hyaluronic acid	1593:1617	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	8	62	dep	simple	1627:1632	arg1	printable					1635:1643	printable	1635:1643	printable	1635:1643	Our results have shown that the combination of methacrylated collagen type I and thiolated hyaluronic acid yield a simple, printable bioink that allows for modulation that was directly related to stromal cell elongation.					
30270846	7	63	theme	liver	1496:1500	arg1	toxicant					1502:1509	a common liver toxicant	1487:1509	a common liver toxicant	1487:1509	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	7	63	theme	liver	1496:1500	arg1	acetaminophen					1472:1484	acetaminophen	1472:1484	acetaminophen	1472:1484	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	6	64	theme	cellular	1182:1189	arg1	behavior					1191:1198	cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity	1182:1308	cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity	1182:1308	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	1	65	theme	tissue	123:128	arg1	printing					111:118	Current 3D printing	100:118	Current 3D printing of tissue	100:128	Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM).					
30270846	7	66	theme	liver	1425:1429	arg1	cells					1440:1444	liver stellate cells	1425:1444	liver stellate cells	1425:1444	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	4	67	from	component	619:627	arg1	ECM					636:638	the ECM	632:638	the ECM	632:638	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	3	68	theme	bioprinted	478:487	arg1	tissues					489:495	the bioprinted tissues	474:495	the bioprinted tissues	474:495	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	3	69	theme	metabolism	553:562	arg1	modeling					524:531	inaccurate modeling	513:531	inaccurate modeling of disease and drug metabolism	513:562	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	4	70	theme	structural	608:617	arg1	type					588:591	Collagen type I	579:593	Collagen type I	579:593	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	4	70	theme	structural	608:617	arg1	component					619:627	the major structural component	598:627	the major structural component in the ECM	598:638	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	6	71	theme	formulations	1101:1112	arg1	properties					1074:1083	the viscoelastic properties	1057:1083	the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid	1057:1166	To test this hypothesis, we tested the viscoelastic properties of three bioink formulations -2:1, 3:1, and 4:1 collagen type I to hyaluronic acid, and examined cellular behavior in order to determine an optimal formulation that allows for bioprinting while supporting biological activity.					
30270846	4	72	theme	low	727:729	arg1	viscosity					731:739	low viscosity	727:739	low viscosity	727:739	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	2	73	theme	composition	312:322	arg1	optimization					296:307	optimization	296:307	optimization of composition and structure of the in vivo tissue microenvironment	296:375	These restrictions have thus far prevented optimization of composition and structure of the in vivo tissue microenvironment.					
30270846	4	74	theme	major	602:606	arg1	type					588:591	Collagen type I	579:593	Collagen type I	579:593	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	4	74	theme	major	602:606	arg1	component					619:627	the major structural component	598:627	the major structural component in the ECM	598:638	Collagen type I is the major structural component in the ECM, and is widely used as a 3D hydrogel, but is less applicable for 3D bioprinting due to low viscosity and slow polymerization.					
30270846	0	75	theme	bioprinted	64:73	arg1	microenvironments					81:97	3D bioprinted liver microenvironments	61:97	3D bioprinted liver microenvironments	61:97	Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments.					
30270846	7	76	theme	stellate	1431:1438	arg1	cells					1440:1444	liver stellate cells	1425:1444	liver stellate cells	1425:1444	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	3	77	theme	inaccurate	513:522	arg1	modeling					524:531	inaccurate modeling	513:531	inaccurate modeling of disease and drug metabolism	513:562	The artificial nature of currently used biomaterials affects cellular phenotype and function of the bioprinted tissues, and results in inaccurate modeling of disease and drug metabolism significantly.					
30270846	1	78	theme	extracellular	225:237	arg1	ECM					247:249	ECM	247:249	ECM	247:249	Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM).					
30270846	1	78	theme	extracellular	225:237	arg1	matrix					239:244	the extracellular matrix	221:244	the extracellular matrix (ECM)	221:250	Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM).					
30270846	0	79	theme	3D	61:62	arg1	microenvironments					81:97	3D bioprinted liver microenvironments	61:97	3D bioprinted liver microenvironments	61:97	Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments.					
30270846	7	80	theme	acetaminophen	1472:1484	arg1	effects					1461:1467	the effects	1457:1467	the effects of acetaminophen, a common liver toxicant	1457:1509	We then employed this formulation to bioprint 3D liver tissue constructs containing primary human hepatocytes and liver stellate cells and tested the effects of acetaminophen, a common liver toxicant.					
30270846	5	81	theme	native	986:991	arg1	microenvironment					993:1008	the native microenvironment	982:1008	the native microenvironment	982:1008	We have hypothesized that a combination of hyaluronic acid with collagen I yields a bioink with the properties required for extrusion bioprinting, while supporting native cell-matrix interactions and preservation of the native microenvironment properties.					
30270846	1	82	theme	matrix	239:244	arg1	properties					207:216	the native properties	196:216	the native properties of the extracellular matrix (ECM)	196:250	Current 3D printing of tissue is restricted by the use of biomaterials that do not recapitulate the native properties of the extracellular matrix (ECM).					
29914979	6	0	dep	two-	955:958	arg1	to					960:961	to	960:961	to	960:961	In mice, monocyte depletion increased binding of PF4 to platelets by two- to three-fold.					
29914979	7	1	theme	3.5-fold	1080:1087	arg1	increase					1089:1096	3.5-fold increase	1080:1096	3.5-fold increase to platelets and rapid transient monocytopenia	1080:1143	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	3	2	theme	murine	526:531	arg1	model					537:541	a murine HIT model	524:541	a murine HIT model	524:541	PF4 binds to monocytes with higher affinity than to platelets, and depletion of monocytes exacerbates thrombocytopenia in a murine HIT model.					
29914979	2	3	from	composition	358:368	arg1	heterogenous					342:353	heterogenous	342:353	heterogenous	342:353	PF4 released from platelets binds to surface glycosaminoglycans on hematopoietic and vascular cells that are heterogenous in composition and differ in affinity for PF4.					
29914979	3	4	theme	HIT	533:535	arg1	model					537:541	a murine HIT model	524:541	a murine HIT model	524:541	PF4 binds to monocytes with higher affinity than to platelets, and depletion of monocytes exacerbates thrombocytopenia in a murine HIT model.					
29914979	2	5	from	heterogenous	342:353	arg1	composition					358:368	composition	358:368	composition	358:368	PF4 released from platelets binds to surface glycosaminoglycans on hematopoietic and vascular cells that are heterogenous in composition and differ in affinity for PF4.					
29914979	7	6	from	increase	1028:1035	arg1	binding					1040:1046	binding	1040:1046	binding of HIT antibody to monocytes	1040:1075	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	4	7	from	development	601:611	arg1	platelets					587:595	platelets	587:595	platelets	587:595	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	2	8	theme	vascular	318:325	arg1	cells					327:331	vascular cells	318:331	vascular cells	318:331	PF4 released from platelets binds to surface glycosaminoglycans on hematopoietic and vascular cells that are heterogenous in composition and differ in affinity for PF4.					
29914979	5	9	theme	PF4	740:742	arg1	Binding					729:735	Binding	729:735	Binding of PF4 to platelets in whole blood in vitro	729:779	Binding of PF4 to platelets in whole blood in vitro varies inversely with the white cell count, likely because of the greater affinity of monocytes for PF4.					
29914979	9	10	theme	platelet	1281:1288	arg1	surfaces					1290:1297	platelet surfaces	1281:1297	platelet surfaces	1281:1297	Exposure of blood to endothelial cells also depletes PF4 from platelet surfaces.					
29914979	10	11	theme	hematopoetic	1375:1386	arg1	cells					1401:1405	hematopoetic and vascular cells	1375:1405	hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT	1375:1496	These studies demonstrate a dynamic interchange of surface-bound PF4 among hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT.					
29914979	4	12	theme	endothelial	702:712	arg1	surfaces					719:726	hematopoietic and endothelial cell surfaces	684:726	hematopoietic and endothelial cell surfaces	684:726	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	2	13	attach	released	237:244	arg2	PF4					233:235	PF4	233:235	PF4 released from platelets	233:259	PF4 released from platelets binds to surface glycosaminoglycans on hematopoietic and vascular cells that are heterogenous in composition and differ in affinity for PF4.					
29914979	2	13	attach	released	237:244	arg1	platelets					251:259	platelets	251:259	platelets	251:259	PF4 released from platelets binds to surface glycosaminoglycans on hematopoietic and vascular cells that are heterogenous in composition and differ in affinity for PF4.					
29914979	7	14	theme	antibody	1055:1062	arg1	binding					1040:1046	binding	1040:1046	binding of HIT antibody to monocytes	1040:1075	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	3	15	theme	monocytes	482:490	arg1	depletion					469:477	depletion	469:477	depletion of monocytes	469:490	PF4 binds to monocytes with higher affinity than to platelets, and depletion of monocytes exacerbates thrombocytopenia in a murine HIT model.					
29914979	1	16	theme	Heparin-induced	96:110	arg1	disorder					154:161	a prothrombotic disorder	138:161	a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes	138:230	Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes.					
29914979	1	16	theme	Heparin-induced	96:110	arg1	HIT					130:132	HIT	130:132	HIT	130:132	Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes.					
29914979	1	16	theme	Heparin-induced	96:110	arg1	thrombocytopenia					112:127	Heparin-induced thrombocytopenia	96:127	Heparin-induced thrombocytopenia (HIT)	96:133	Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes.					
29914979	1	17	theme	factor	199:204	arg1	complexes					222:230	platelet factor 4 (PF4)/heparin complexes	190:230	platelet factor 4 (PF4)/heparin complexes	190:230	Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes.					
29914979	8	18	from	return	1192:1197	arg1	counts					1211:1216	platelet counts	1202:1216	platelet counts	1202:1216	Normalization of monocyte counts preceded the return in platelet counts.					
29914979	3	19	theme	higher	430:435	arg1	affinity					437:444	higher affinity	430:444	higher affinity than to platelets	430:462	PF4 binds to monocytes with higher affinity than to platelets, and depletion of monocytes exacerbates thrombocytopenia in a murine HIT model.					
29914979	5	20	theme	white	807:811	arg1	count					818:822	the white cell count	803:822	the white cell count	803:822	Binding of PF4 to platelets in whole blood in vitro varies inversely with the white cell count, likely because of the greater affinity of monocytes for PF4.					
29914979	5	21	theme	greater	847:853	arg1	affinity					855:862	the greater affinity	843:862	the greater affinity of monocytes for PF4	843:883	Binding of PF4 to platelets in whole blood in vitro varies inversely with the white cell count, likely because of the greater affinity of monocytes for PF4.					
29914979	0	22	theme	intercellular	8:20	arg1	redistribution					22:35	Dynamic intercellular redistribution	0:35	Dynamic intercellular redistribution of HIT antigen	0:50	Dynamic intercellular redistribution of HIT antigen modulates heparin-induced thrombocytopenia.					
29914979	6	23	theme	PF4	935:937	arg1	binding					924:930	binding	924:930	binding of PF4 to platelets	924:950	In mice, monocyte depletion increased binding of PF4 to platelets by two- to three-fold.					
29914979	4	24	theme	PF4	674:676	arg1	distribution					658:669	the (re)distribution	650:669	the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces	650:726	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	0	25	theme	Dynamic	0:6	arg1	redistribution					22:35	Dynamic intercellular redistribution	0:35	Dynamic intercellular redistribution of HIT antigen	0:50	Dynamic intercellular redistribution of HIT antigen modulates heparin-induced thrombocytopenia.					
29914979	7	26	theme	HIT	1051:1053	arg1	antibody					1055:1062	HIT antibody	1051:1062	HIT antibody	1051:1062	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	4	27	from	expression	566:575	arg1	platelets					587:595	platelets	587:595	platelets	587:595	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	10	28	theme	dynamic	1328:1334	arg1	interchange					1336:1346	a dynamic interchange	1326:1346	a dynamic interchange of surface-bound PF4 among hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT	1326:1496	These studies demonstrate a dynamic interchange of surface-bound PF4 among hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT.					
29914979	10	29	theme	vascular	1392:1399	arg1	cells					1401:1405	hematopoetic and vascular cells	1375:1405	hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT	1375:1496	These studies demonstrate a dynamic interchange of surface-bound PF4 among hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT.					
29914979	7	30	from	Induction	975:983	arg1	mice					995:998	mice	995:998	mice	995:998	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	8	31	theme	counts	1172:1177	arg1	Normalization					1146:1158	Normalization	1146:1158	Normalization of monocyte counts	1146:1177	Normalization of monocyte counts preceded the return in platelet counts.					
29914979	4	32	theme	PF4	580:582	arg1	expression					566:575	the expression	562:575	the expression of PF4 on platelets	562:595	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	4	32	theme	PF4	580:582	arg1	development					601:611	development	601:611	development of thrombocytopenia	601:631	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	1	33	theme	platelet	190:197	arg1	factor					199:204	platelet factor 4	190:206	platelet factor 4 (PF4)/heparin complexes	190:230	Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes.					
29914979	1	33	theme	platelet	190:197	arg1	PF4					209:211	PF4	209:211	PF4	209:211	Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes.					
29914979	1	34	theme	/heparin	213:220	arg1	complexes					222:230	platelet factor 4 (PF4)/heparin complexes	190:230	platelet factor 4 (PF4)/heparin complexes	190:230	Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes.					
29914979	0	35	theme	antigen	44:50	arg1	redistribution					22:35	Dynamic intercellular redistribution	0:35	Dynamic intercellular redistribution of HIT antigen	0:50	Dynamic intercellular redistribution of HIT antigen modulates heparin-induced thrombocytopenia.					
29914979	4	36	theme	cell	714:717	arg1	surfaces					719:726	hematopoietic and endothelial cell surfaces	684:726	hematopoietic and endothelial cell surfaces	684:726	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	7	37	theme	>80-fold	1019:1026	arg1	increase					1028:1035	a transient >80-fold increase	1007:1035	a transient >80-fold increase in binding of HIT antibody to monocytes	1007:1075	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	5	38	from	platelets	747:755	arg1	blood					766:770	whole blood	760:770	whole blood	760:770	Binding of PF4 to platelets in whole blood in vitro varies inversely with the white cell count, likely because of the greater affinity of monocytes for PF4.					
29914979	4	39	theme	re	655:656	arg1	distribution					658:669	the (re)distribution	650:669	the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces	650:726	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	0	40	theme	HIT	40:42	arg1	antigen					44:50	HIT antigen	40:50	HIT antigen	40:50	Dynamic intercellular redistribution of HIT antigen modulates heparin-induced thrombocytopenia.					
29914979	7	41	theme	rapid	1115:1119	arg1	monocytopenia					1131:1143	rapid transient monocytopenia	1115:1143	rapid transient monocytopenia	1115:1143	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	5	42	theme	cell	813:816	arg1	count					818:822	the white cell count	803:822	the white cell count	803:822	Binding of PF4 to platelets in whole blood in vitro varies inversely with the white cell count, likely because of the greater affinity of monocytes for PF4.					
29914979	7	43	theme	HIT	988:990	arg1	Induction					975:983	Induction	975:983	Induction of HIT in mice	975:998	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	8	44	theme	monocyte	1163:1170	arg1	counts					1172:1177	monocyte counts	1163:1177	monocyte counts	1163:1177	Normalization of monocyte counts preceded the return in platelet counts.					
29914979	0	45	theme	heparin-induced	62:76	arg1	thrombocytopenia					78:93	heparin-induced thrombocytopenia	62:93	heparin-induced thrombocytopenia	62:93	Dynamic intercellular redistribution of HIT antigen modulates heparin-induced thrombocytopenia.					
29914979	5	46	theme	monocytes	867:875	arg1	affinity					855:862	the greater affinity	843:862	the greater affinity of monocytes for PF4	843:883	Binding of PF4 to platelets in whole blood in vitro varies inversely with the white cell count, likely because of the greater affinity of monocytes for PF4.					
29914979	7	47	dep	rapid	1115:1119	arg1	transient					1121:1129	transient	1121:1129	transient	1121:1129	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	7	48	theme	transient	1009:1017	arg1	increase					1028:1035	a transient >80-fold increase	1007:1035	a transient >80-fold increase in binding of HIT antibody to monocytes	1007:1075	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	9	49	theme	blood	1231:1235	arg1	Exposure					1219:1226	Exposure	1219:1226	Exposure of blood to endothelial cells	1219:1256	Exposure of blood to endothelial cells also depletes PF4 from platelet surfaces.					
29914979	10	50	theme	prothrombotic	1467:1479	arg1	processes					1481:1489	prothrombotic processes	1467:1489	prothrombotic processes	1467:1489	These studies demonstrate a dynamic interchange of surface-bound PF4 among hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT.					
29914979	7	51	from	HIT	988:990	arg1	mice					995:998	mice	995:998	mice	995:998	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
29914979	1	52	theme	prothrombotic	140:152	arg1	disorder					154:161	a prothrombotic disorder	138:161	a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes	138:230	Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes.					
29914979	1	52	theme	prothrombotic	140:152	arg1	thrombocytopenia					112:127	Heparin-induced thrombocytopenia	96:127	Heparin-induced thrombocytopenia (HIT)	96:133	Heparin-induced thrombocytopenia (HIT) is a prothrombotic disorder initiated by antibodies to platelet factor 4 (PF4)/heparin complexes.					
29914979	4	53	theme	thrombocytopenia	616:631	arg1	expression					566:575	the expression	562:575	the expression of PF4 on platelets	562:595	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	4	53	theme	thrombocytopenia	616:631	arg1	development					601:611	development	601:611	development of thrombocytopenia	601:631	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	9	54	theme	endothelial	1240:1250	arg1	cells					1252:1256	endothelial cells	1240:1256	endothelial cells	1240:1256	Exposure of blood to endothelial cells also depletes PF4 from platelet surfaces.					
29914979	2	55	theme	surface	270:276	arg1	glycosaminoglycans					278:295	surface glycosaminoglycans	270:295	surface glycosaminoglycans	270:295	PF4 released from platelets binds to surface glycosaminoglycans on hematopoietic and vascular cells that are heterogenous in composition and differ in affinity for PF4.					
29914979	10	56	theme	PF4	1365:1367	arg1	interchange					1336:1346	a dynamic interchange	1326:1346	a dynamic interchange of surface-bound PF4 among hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT	1326:1496	These studies demonstrate a dynamic interchange of surface-bound PF4 among hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT.					
29914979	8	57	theme	platelet	1202:1209	arg1	counts					1211:1216	platelet counts	1202:1216	platelet counts	1202:1216	Normalization of monocyte counts preceded the return in platelet counts.					
29914979	10	58	theme	surface-bound	1351:1363	arg1	PF4					1365:1367	surface-bound PF4	1351:1367	surface-bound PF4	1351:1367	These studies demonstrate a dynamic interchange of surface-bound PF4 among hematopoetic and vascular cells that may limit thrombocytopenia at the expense of promoting prothrombotic processes in HIT.					
29914979	4	59	theme	hematopoietic	684:696	arg1	surfaces					719:726	hematopoietic and endothelial cell surfaces	684:726	hematopoietic and endothelial cell surfaces	684:726	Here we show that the expression of PF4 on platelets and development of thrombocytopenia are modulated by the (re)distribution of PF4 among hematopoietic and endothelial cell surfaces.					
29914979	5	60	theme	whole	760:764	arg1	blood					766:770	whole blood	760:770	whole blood	760:770	Binding of PF4 to platelets in whole blood in vitro varies inversely with the white cell count, likely because of the greater affinity of monocytes for PF4.					
29914979	6	61	theme	monocyte	895:902	arg1	depletion					904:912	monocyte depletion	895:912	monocyte depletion	895:912	In mice, monocyte depletion increased binding of PF4 to platelets by two- to three-fold.					
29914979	9	62	from	surfaces	1290:1297	arg1	PF4					1272:1274	PF4	1272:1274	PF4 from platelet surfaces	1272:1297	Exposure of blood to endothelial cells also depletes PF4 from platelet surfaces.					
29914979	7	63	from	mice	995:998	arg1	Induction					975:983	Induction	975:983	Induction of HIT in mice	975:998	Induction of HIT in mice caused a transient >80-fold increase in binding of HIT antibody to monocytes vs 3.5-fold increase to platelets and rapid transient monocytopenia.					
31595797	7	0	theme	calcium	1219:1225	arg1	deposition					1227:1236	calcium deposition	1219:1236	calcium deposition	1219:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	6	1	theme	surface	910:916	arg1	morphology					918:927	surface morphology	910:927	surface morphology	910:927	The surface morphology, hydrophilicity, tensile strength and the bioactivity of the scaffolds were characterized.					
31595797	7	2	theme	osteogenic	1134:1143	arg1	activity					1158:1165	the activity	1154:1165	the activity of the enzyme alkaline phosphatase	1154:1200	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	2	theme	osteogenic	1134:1143	arg1	level					1210:1214	the level	1206:1214	the level of calcium deposition	1206:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	2	theme	osteogenic	1134:1143	arg1	markers					1145:1151	the basic osteogenic markers	1124:1151	the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition	1124:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	8	3	contain	have	1314:1317	arg2	potential					1339:1347	a better osteogenic potential	1319:1347	a better osteogenic potential than pure PLLA scaffolds	1319:1372	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	8	3	contain	have	1314:1317	arg1	scaffolds					1253:1261	The composite scaffolds	1239:1261	The composite scaffolds prepared here	1239:1275	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	2	4	theme	acid	423:426	arg1	scaffold					397:404	a composite scaffold	385:404	a composite scaffold of Poly-l-lactic acid (PLLA)	385:433	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	1	5	theme	tissue	312:317	arg1	application					331:341	tissue engineering application	312:341	tissue engineering application	312:341	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	2	6	theme	engineering	591:601	arg1	application					603:613	bone tissue engineering application	579:613	bone tissue engineering application	579:613	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	2	7	theme	Poly-l-lactic	409:421	arg1	acid					423:426	Poly-l-lactic acid	409:426	Poly-l-lactic acid (PLLA)	409:433	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	2	7	theme	Poly-l-lactic	409:421	arg1	PLLA					429:432	PLLA	429:432	PLLA	429:432	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	1	8	theme	engineering	319:329	arg1	application					331:341	tissue engineering application	312:341	tissue engineering application	312:341	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	0	9	theme	osteogenic	128:137	arg1	differentiation					139:153	enhanced osteogenic differentiation	119:153	enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery	119:214	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	8	10	contain	have	1277:1280	arg2	property					1301:1308	conductive surface property	1282:1308	conductive surface property	1282:1308	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	8	10	contain	have	1277:1280	arg1	scaffolds					1253:1261	The composite scaffolds	1239:1261	The composite scaffolds prepared here	1239:1275	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	9	11	contain	containing	1421:1430	arg2	Dexa					1432:1435	Dexa	1432:1435	Dexa	1432:1435	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	9	11	contain	containing	1421:1430	arg1	nanoparticle					1408:1419	nanoparticle	1408:1419	nanoparticle containing Dexa from composite scaffold	1408:1459	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	6	12	dep	morphology	918:927	arg1	The					906:908	The	906:908	The	906:908	The surface morphology, hydrophilicity, tensile strength and the bioactivity of the scaffolds were characterized.					
31595797	0	13	theme	enhanced	119:126	arg1	differentiation					139:153	enhanced osteogenic differentiation	119:153	enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery	119:214	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	9	14	theme	tissue	1547:1552	arg1	engineering					1554:1564	bone tissue engineering application and pH-sensitive delivery	1542:1602	engineering	1554:1564	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	7	15	theme	basic	1128:1132	arg1	activity					1158:1165	the activity	1154:1165	the activity of the enzyme alkaline phosphatase	1154:1200	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	15	theme	basic	1128:1132	arg1	level					1210:1214	the level	1206:1214	the level of calcium deposition	1206:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	15	theme	basic	1128:1132	arg1	markers					1145:1151	the basic osteogenic markers	1124:1151	the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition	1124:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	8	16	theme	PLLA	1359:1362	arg1	scaffolds					1364:1372	pure PLLA scaffolds	1354:1372	pure PLLA scaffolds	1354:1372	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	8	17	theme	conductive	1282:1291	arg1	property					1301:1308	conductive surface property	1282:1308	conductive surface property	1282:1308	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	9	18	theme	drugs	1607:1611	arg1	delivery					1595:1602	pH-sensitive delivery	1582:1602	pH-sensitive delivery	1582:1602	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	9	18	theme	drugs	1607:1611	arg1	engineering					1554:1564	bone tissue engineering application and pH-sensitive delivery	1542:1602	engineering	1554:1564	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	5	19	theme	MSN-Dexa	800:807	arg1	CS					809:810	MSN-Dexa@CS	800:810	MSN-Dexa@CS	800:810	In the following, CS was coated over the prepared nanoparticles to form MSN-Dexa@CS and then, were mixed to PLLA solution to form MSN-Dexa@CS/PLLA composite for electrospinning.					
31595797	9	20	theme	composite	1442:1450	arg1	scaffold					1452:1459	composite scaffold	1442:1459	composite scaffold	1442:1459	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	7	21	theme	MTT	1097:1099	arg1	assay					1101:1105	MTT assay	1097:1105	MTT assay	1097:1105	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	22	theme	phosphatase	1190:1200	arg1	activity					1158:1165	the activity	1154:1165	the activity of the enzyme alkaline phosphatase	1154:1200	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	22	theme	phosphatase	1190:1200	arg1	level					1210:1214	the level	1206:1214	the level of calcium deposition	1206:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	22	theme	phosphatase	1190:1200	arg1	markers					1145:1151	the basic osteogenic markers	1124:1151	the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition	1124:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	8	23	theme	composite	1243:1251	arg1	scaffolds					1253:1261	The composite scaffolds	1239:1261	The composite scaffolds prepared here	1239:1275	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	0	24	theme	acid	14:17	arg1	scaffold					19:26	Poly-l-lactic acid scaffold	0:26	Poly-l-lactic acid scaffold	0:26	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	7	25	theme	deposition	1227:1236	arg1	activity					1158:1165	the activity	1154:1165	the activity of the enzyme alkaline phosphatase	1154:1200	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	25	theme	deposition	1227:1236	arg1	level					1210:1214	the level	1206:1214	the level of calcium deposition	1206:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	25	theme	deposition	1227:1236	arg1	markers					1145:1151	the basic osteogenic markers	1124:1151	the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition	1124:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	26	theme	alkaline	1181:1188	arg1	phosphatase					1190:1200	the enzyme alkaline phosphatase	1170:1200	the enzyme alkaline phosphatase	1170:1200	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	5	27	theme	MSN-Dexa	858:865	arg1	CS/PLLA					867:873	MSN-Dexa@CS/PLLA	858:873	MSN-Dexa@CS/PLLA composite for electrospinning	858:903	In the following, CS was coated over the prepared nanoparticles to form MSN-Dexa@CS and then, were mixed to PLLA solution to form MSN-Dexa@CS/PLLA composite for electrospinning.					
31595797	0	28	theme	Poly-l-lactic	0:12	arg1	scaffold					19:26	Poly-l-lactic acid scaffold	0:26	Poly-l-lactic acid scaffold	0:26	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	9	29	theme	bone	1542:1545	arg1	engineering					1554:1564	bone tissue engineering application and pH-sensitive delivery	1542:1602	engineering	1554:1564	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	5	30	theme	@	866:866	arg1	CS/PLLA					867:873	MSN-Dexa@CS/PLLA	858:873	MSN-Dexa@CS/PLLA composite for electrospinning	858:903	In the following, CS was coated over the prepared nanoparticles to form MSN-Dexa@CS and then, were mixed to PLLA solution to form MSN-Dexa@CS/PLLA composite for electrospinning.					
31595797	2	31	theme	tissue	584:589	arg1	application					603:613	bone tissue engineering application	579:613	bone tissue engineering application	579:613	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	1	32	theme	attractive	349:358	arg1	approach					360:367	an attractive approach	346:367	an attractive approach	346:367	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	1	32	theme	attractive	349:358	arg1	Nowadays					217:224	Nowadays	217:224	Nowadays	217:224	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	1	32	theme	attractive	349:358	arg1	development					231:241	the development	227:241	the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application	227:341	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	0	33	theme	adipose	164:170	arg1	cells					184:188	human adipose tissue stem cells	158:188	human adipose tissue stem cells	158:188	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	9	34	dep	engineering	1554:1564	arg1	application					1566:1576	application	1566:1576	application	1566:1576	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	2	35	theme	Mesoporous	476:485	arg1	MSN					509:511	MSN	509:511	MSN	509:511	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	2	35	theme	Mesoporous	476:485	arg1	Nanoparticles					494:506	Mesoporous Silica Nanoparticles	476:506	Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS)	476:538	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	2	36	theme	Silica	487:492	arg1	MSN					509:511	MSN	509:511	MSN	509:511	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	2	36	theme	Silica	487:492	arg1	Nanoparticles					494:506	Mesoporous Silica Nanoparticles	476:506	Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS)	476:538	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	0	37	theme	human	158:162	arg1	cells					184:188	human adipose tissue stem cells	158:188	human adipose tissue stem cells	158:188	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	9	38	theme	implantation	1628:1639	arg1	site					1620:1623	the site	1616:1623	the site of implantation in tissue regeneration	1616:1662	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	9	38	theme	implantation	1628:1639	arg1	implantation					1628:1639	implantation	1628:1639	implantation	1628:1639	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	9	39	theme	pH-sensitive	1582:1593	arg1	delivery					1595:1602	pH-sensitive delivery	1582:1602	pH-sensitive delivery	1582:1602	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	9	40	theme	composite	1501:1509	arg1	candidates					1527:1536	the composite scaffolds ideal candidates	1497:1536	the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs	1497:1611	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	0	41	theme	mesoporous	57:66	arg1	nanoparticles					75:87	chitosan-coated mesoporous silica nanoparticles	41:87	chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery	41:214	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	0	42	theme	stem	179:182	arg1	cells					184:188	human adipose tissue stem cells	158:188	human adipose tissue stem cells	158:188	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	5	43	theme	PLLA	836:839	arg1	solution					841:848	PLLA solution	836:848	PLLA solution	836:848	In the following, CS was coated over the prepared nanoparticles to form MSN-Dexa@CS and then, were mixed to PLLA solution to form MSN-Dexa@CS/PLLA composite for electrospinning.					
31595797	3	44	theme	precipitation	640:652	arg1	method					654:659	precipitation method	640:659	precipitation method	640:659	The MSN was prepared by precipitation method.					
31595797	8	45	theme	better	1321:1326	arg1	potential					1339:1347	a better osteogenic potential	1319:1347	a better osteogenic potential than pure PLLA scaffolds	1319:1372	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	1	46	theme	drug-loaded	246:256	arg1	scaffolds					298:306	drug-loaded electrospun organic-inorganic composite scaffolds	246:306	drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application	246:341	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	0	47	theme	chitosan-coated	41:55	arg1	nanoparticles					75:87	chitosan-coated mesoporous silica nanoparticles	41:87	chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery	41:214	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	6	48	theme	tensile	946:952	arg1	strength					954:961	tensile strength	946:961	tensile strength	946:961	The surface morphology, hydrophilicity, tensile strength and the bioactivity of the scaffolds were characterized.					
31595797	0	49	theme	tissue	172:177	arg1	cells					184:188	human adipose tissue stem cells	158:188	human adipose tissue stem cells	158:188	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	6	50	theme	scaffolds	990:998	arg1	bioactivity					971:981	the bioactivity	967:981	the bioactivity	967:981	The surface morphology, hydrophilicity, tensile strength and the bioactivity of the scaffolds were characterized.					
31595797	6	50	theme	scaffolds	990:998	arg1	hydrophilicity					930:943	hydrophilicity	930:943	hydrophilicity	930:943	The surface morphology, hydrophilicity, tensile strength and the bioactivity of the scaffolds were characterized.					
31595797	6	50	theme	scaffolds	990:998	arg1	strength					954:961	tensile strength	946:961	tensile strength	946:961	The surface morphology, hydrophilicity, tensile strength and the bioactivity of the scaffolds were characterized.					
31595797	6	50	theme	scaffolds	990:998	arg1	morphology					918:927	surface morphology	910:927	surface morphology	910:927	The surface morphology, hydrophilicity, tensile strength and the bioactivity of the scaffolds were characterized.					
31595797	9	51	theme	tissue	1644:1649	arg1	regeneration					1651:1662	tissue regeneration	1644:1662	tissue regeneration	1644:1662	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	2	52	theme	bone	579:582	arg1	application					603:613	bone tissue engineering application	579:613	bone tissue engineering application	579:613	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	1	53	theme	electrospun	258:268	arg1	scaffolds					298:306	drug-loaded electrospun organic-inorganic composite scaffolds	246:306	drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application	246:341	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	5	54	theme	@	808:808	arg1	CS					809:810	MSN-Dexa@CS	800:810	MSN-Dexa@CS	800:810	In the following, CS was coated over the prepared nanoparticles to form MSN-Dexa@CS and then, were mixed to PLLA solution to form MSN-Dexa@CS/PLLA composite for electrospinning.					
31595797	7	55	theme	differentiation	1053:1067	arg1	potential					1069:1077	The osteogenic proliferation and differentiation potential	1020:1077	The osteogenic proliferation and differentiation potential	1020:1077	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	9	56	theme	scaffolds	1511:1519	arg1	candidates					1527:1536	the composite scaffolds ideal candidates	1497:1536	the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs	1497:1611	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	7	57	theme	osteogenic	1024:1033	arg1	potential					1069:1077	The osteogenic proliferation and differentiation potential	1020:1077	The osteogenic proliferation and differentiation potential	1020:1077	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	0	58	theme	silica	68:73	arg1	nanoparticles					75:87	chitosan-coated mesoporous silica nanoparticles	41:87	chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery	41:214	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	9	59	theme	controlled	1386:1395	arg1	release					1397:1403	the controlled release	1382:1403	the controlled release of nanoparticle containing Dexa from composite scaffold	1382:1459	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	0	60	theme	cells	184:188	arg1	differentiation					139:153	enhanced osteogenic differentiation	119:153	enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery	119:214	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	7	61	theme	proliferation	1035:1047	arg1	potential					1069:1077	The osteogenic proliferation and differentiation potential	1020:1077	The osteogenic proliferation and differentiation potential	1020:1077	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	9	62	from	site	1620:1623	arg1	regeneration					1651:1662	tissue regeneration	1644:1662	tissue regeneration	1644:1662	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	2	63	theme	composite	387:395	arg1	scaffold					397:404	a composite scaffold	385:404	a composite scaffold of Poly-l-lactic acid (PLLA)	385:433	In this study, a composite scaffold of Poly-l-lactic acid (PLLA) incorporated dexamethasone (Dexa) loaded Mesoporous Silica Nanoparticles (MSN) coated with Chitosan (CS) were fabricated by electrospinning for bone tissue engineering application.					
31595797	1	64	theme	organic-inorganic	270:286	arg1	scaffolds					298:306	drug-loaded electrospun organic-inorganic composite scaffolds	246:306	drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application	246:341	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	0	65	theme	dexamethasone	193:205	arg1	delivery					207:214	dexamethasone delivery	193:214	dexamethasone delivery	193:214	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	9	66	theme	nanoparticle	1408:1419	arg1	release					1397:1403	the controlled release	1382:1403	the controlled release of nanoparticle containing Dexa from composite scaffold	1382:1459	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	8	67	theme	osteogenic	1328:1337	arg1	potential					1339:1347	a better osteogenic potential	1319:1347	a better osteogenic potential than pure PLLA scaffolds	1319:1372	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	8	68	theme	pure	1354:1357	arg1	scaffolds					1364:1372	pure PLLA scaffolds	1354:1372	pure PLLA scaffolds	1354:1372	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	1	69	theme	composite	288:296	arg1	scaffolds					298:306	drug-loaded electrospun organic-inorganic composite scaffolds	246:306	drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application	246:341	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	0	70	theme	pH-sensitive	92:103	arg1	composite					105:113	pH-sensitive composite	92:113	pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery	92:214	Poly-l-lactic acid scaffold incorporated chitosan-coated mesoporous silica nanoparticles as pH-sensitive composite for enhanced osteogenic differentiation of human adipose tissue stem cells by dexamethasone delivery.					
31595797	8	71	theme	surface	1293:1299	arg1	property					1301:1308	conductive surface property	1282:1308	conductive surface property	1282:1308	The composite scaffolds prepared here have conductive surface property and have a better osteogenic potential than pure PLLA scaffolds.					
31595797	7	72	dep	markers	1145:1151	arg1	activity					1158:1165	the activity	1154:1165	the activity of the enzyme alkaline phosphatase	1154:1200	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	72	dep	markers	1145:1151	arg1	level					1210:1214	the level	1206:1214	the level of calcium deposition	1206:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	7	72	dep	markers	1145:1151	arg1	markers					1145:1151	the basic osteogenic markers	1124:1151	the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition	1124:1236	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	9	73	theme	ideal	1521:1525	arg1	candidates					1527:1536	the composite scaffolds ideal candidates	1497:1536	the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs	1497:1611	Hence, the controlled release of nanoparticle containing Dexa from composite scaffold supported the osteogenesis and made the composite scaffolds ideal candidates for bone tissue engineering application and pH-sensitive delivery of drugs at the site of implantation in tissue regeneration.					
31595797	1	74	theme	scaffolds	298:306	arg1	approach					360:367	an attractive approach	346:367	an attractive approach	346:367	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	1	74	theme	scaffolds	298:306	arg1	Nowadays					217:224	Nowadays	217:224	Nowadays	217:224	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	1	74	theme	scaffolds	298:306	arg1	development					231:241	the development	227:241	the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application	227:341	Nowadays, the development of drug-loaded electrospun organic-inorganic composite scaffolds for tissue engineering application is an attractive approach.					
31595797	5	75	theme	prepared	769:776	arg1	nanoparticles					778:790	the prepared nanoparticles	765:790	the prepared nanoparticles	765:790	In the following, CS was coated over the prepared nanoparticles to form MSN-Dexa@CS and then, were mixed to PLLA solution to form MSN-Dexa@CS/PLLA composite for electrospinning.					
31595797	7	76	theme	enzyme	1174:1179	arg1	phosphatase					1190:1200	the enzyme alkaline phosphatase	1170:1200	the enzyme alkaline phosphatase	1170:1200	The osteogenic proliferation and differentiation potential were evaluated by MTT assay and by measuring the basic osteogenic markers: the activity of the enzyme alkaline phosphatase and the level of calcium deposition.					
31595797	5	77	theme	composite	875:883	arg1	CS/PLLA					867:873	MSN-Dexa@CS/PLLA	858:873	MSN-Dexa@CS/PLLA composite for electrospinning	858:903	In the following, CS was coated over the prepared nanoparticles to form MSN-Dexa@CS and then, were mixed to PLLA solution to form MSN-Dexa@CS/PLLA composite for electrospinning.					
29668036	0	0	from	Extraction	16:25	arg1	Seeds					66:70	Grape Seeds	60:70	Grape Seeds	60:70	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	1	1	with	full	159:162	arg1	oil					169:171	oil	169:171	oil	169:171	Grape seeds full with oil and also rich in hypoglycemic ingredients.					
29668036	3	2	from	seeds	423:427	arg1	extracts					446:453	water soluble extracts	432:453	water soluble extracts containing TP, TC, PC contents, respectively	432:498	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	1	3	theme	full	159:162	arg1	seeds					153:157	Grape seeds	147:157	Grape seeds full with oil	147:171	Grape seeds full with oil and also rich in hypoglycemic ingredients.					
29668036	7	4	theme	hypoglycemic	1300:1311	arg1	activity					1313:1320	hypoglycemic activity	1300:1320	hypoglycemic activity	1300:1320	The optimized extraction, composition of constituents and hypoglycemic activity were investigated in this study.					
29668036	3	5	from	seeds	395:399	arg1	oil					380:382	extracting oil	369:382	extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively,	369:499	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	4	6	contain	containing	583:592	arg2	epicatechin					607:617	epicatechin	607:617	epicatechin	607:617	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	6	contain	containing	583:592	arg2	catechin					594:601	catechin	594:601	catechin	594:601	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	6	contain	containing	583:592	arg1	samples					575:581	GSAE samples	570:581	GSAE samples containing catechin and epicatechin	570:617	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	8	7	from	polyphenols	1421:1431	arg1	rich					1396:1399	rich	1396:1399	rich	1396:1399	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	3	8	theme	PC	474:475	arg1	contents					477:484	PC contents	474:484	PC contents	474:484	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	3	8	theme	PC	474:475	arg1	TP					466:467	TP	466:467	TP	466:467	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	8	9	theme	α-glucosidase	1472:1484	arg1	activity					1500:1507	α-glucosidase and α-amylase activity	1472:1507	α-glucosidase and α-amylase activity	1472:1507	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	3	10	theme	active	344:349	arg1	±					511:511	171.1 ± 1.1	505:515	171.1 ± 1.1	505:515	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	3	10	theme	active	344:349	arg1	substances					351:360	Extract water soluble active substances	322:360	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively,	322:499	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	3	11	theme	mg	544:545	arg1	GSAE/g					547:552	mg GSAE/g	544:552	mg GSAE/g	544:552	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	3	11	theme	mg	544:545	arg1	±					524:524	658.8 ± 0.2	518:528	658.8 ± 0.2	518:528	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	4	12	theme	±	760:760	arg1	g/mL					766:769	66.68 ± 1.1 g/mL	754:769	66.68 ± 1.1 g/mL	754:769	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	5	13	theme	diabetes	1099:1106	arg1	treatment					1086:1094	treatment	1086:1094	treatment	1086:1094	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	5	13	theme	diabetes	1099:1106	arg1	prevention					1071:1080	prevention	1071:1080	prevention	1071:1080	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	0	14	theme	α-Glucosidase	90:102	arg1	Effects					129:135	α-Glucosidase and α-Amylase Inhibitory Effects	90:135	α-Glucosidase and α-Amylase Inhibitory Effects In Vitro	90:144	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	5	15	theme	grape	941:945	arg1	seeds					947:951	the grape seeds	937:951	the grape seeds	937:951	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	8	16	theme	added	1616:1620	arg1	value					1622:1626	high added value	1611:1626	high added value	1611:1626	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	8	17	theme	α-amylase	1490:1498	arg1	activity					1500:1507	α-glucosidase and α-amylase activity	1472:1507	α-glucosidase and α-amylase activity	1472:1507	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	2	18	theme	seeds	280:284	arg1	quality					294:300	the quality	290:300	the quality of grape seeds oil	290:319	This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil.					
29668036	2	18	theme	seeds	280:284	arg1	utilization					259:269	the comprehensive utilization	241:269	the comprehensive utilization of grape seeds	241:284	This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil.					
29668036	8	19	theme	deep-processed	1567:1580	arg1	products					1582:1589	the deep-processed products	1563:1589	the deep-processed products of grape seeds with high added value	1563:1626	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	4	20	dep	mg/mL	637:641	arg1	both					772:775	both	772:775	both	772:775	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	0	21	theme	Inhibitory	118:127	arg1	Effects					129:135	α-Glucosidase and α-Amylase Inhibitory Effects	90:135	α-Glucosidase and α-Amylase Inhibitory Effects In Vitro	90:144	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	3	22	contain	containing	455:464	arg2	contents					477:484	PC contents	474:484	PC contents	474:484	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	3	22	contain	containing	455:464	arg2	TC					470:471	TC	470:471	TC	470:471	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	3	22	contain	containing	455:464	arg2	TP					466:467	TP	466:467	TP	466:467	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	3	22	contain	containing	455:464	arg1	extracts					446:453	water soluble extracts	432:453	water soluble extracts containing TP, TC, PC contents, respectively	432:498	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	2	23	theme	grape	274:278	arg1	seeds					280:284	grape seeds	274:284	grape seeds	274:284	This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil.					
29668036	3	24	theme	grape	417:421	arg1	seeds					423:427	the grape seeds	413:427	the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively	413:498	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	0	25	theme	α-Amylase	108:116	arg1	Effects					129:135	α-Glucosidase and α-Amylase Inhibitory Effects	90:135	α-Glucosidase and α-Amylase Inhibitory Effects In Vitro	90:144	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	6	26	from	rich	1200:1203	arg1	polyphenol					1208:1217	polyphenol	1208:1217	polyphenol	1208:1217	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	6	26	from	rich	1200:1203	arg1	ingredients					1229:1239	other ingredients	1223:1239	other ingredients	1223:1239	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	6	27	theme	wines	1189:1193	arg1	by-products					1166:1176	by-products	1166:1176	by-products of brewing wines	1166:1193	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	6	27	theme	wines	1189:1193	arg1	seeds					1159:1163	PRACTICAL APPLICATION Grape seeds	1131:1163	PRACTICAL APPLICATION Grape seeds	1131:1163	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	4	28	theme	Inhibitory	814:823	arg1	better					840:845	better	840:845	better	840:845	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	28	theme	Inhibitory	814:823	arg1	effects					803:809	the effects	799:809	the effects of Inhibitory	799:823	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	8	29	theme	grape	1379:1383	arg1	seeds					1385:1389	grape seeds	1379:1389	grape seeds	1379:1389	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	4	30	theme	α-amylase	735:743	arg1	IC50					745:748	α-amylase IC50	735:748	α-amylase IC50	735:748	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	2	31	theme	grape	305:309	arg1	seeds					311:315	grape seeds	305:315	grape seeds oil	305:319	This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil.					
29668036	6	32	theme	Grape	1153:1157	arg1	by-products					1166:1176	by-products	1166:1176	by-products of brewing wines	1166:1193	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	6	32	theme	Grape	1153:1157	arg1	seeds					1159:1163	PRACTICAL APPLICATION Grape seeds	1131:1163	PRACTICAL APPLICATION Grape seeds	1131:1163	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	5	33	used	used	1034:1037	arg2	GSAE					1022:1025	GSAE	1022:1025	GSAE	1022:1025	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	6	34	theme	brewing	1181:1187	arg1	wines					1189:1193	brewing wines	1181:1193	brewing wines	1181:1193	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	7	35	theme	optimized	1246:1254	arg1	composition					1268:1278	composition	1268:1278	composition	1268:1278	The optimized extraction, composition of constituents and hypoglycemic activity were investigated in this study.					
29668036	7	35	theme	optimized	1246:1254	arg1	extraction					1256:1265	extraction	1256:1265	extraction	1256:1265	The optimized extraction, composition of constituents and hypoglycemic activity were investigated in this study.					
29668036	8	36	theme	seeds	1600:1604	arg1	products					1582:1589	the deep-processed products	1563:1589	the deep-processed products of grape seeds with high added value	1563:1626	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	4	37	theme	GSAE	570:573	arg1	samples					575:581	GSAE samples	570:581	GSAE samples containing catechin and epicatechin	570:617	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	38	theme	competitive	782:792	arg1	way					794:796	competitive way	782:796	competitive way	782:796	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	39	theme	±	651:651	arg1	mg/g					658:661	111.23 ± 1.29 mg/g	644:661	111.23 ± 1.29 mg/g	644:661	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	39	theme	±	651:651	arg1	mg/mL					637:641	44.12 ± 0.21 mg/mL	624:641	44.12 ± 0.21 mg/mL	624:641	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	8	40	theme	grape	1594:1598	arg1	seeds					1600:1604	grape seeds	1594:1604	grape seeds with high added value	1594:1626	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	3	41	theme	soluble	438:444	arg1	extracts					446:453	water soluble extracts	432:453	water soluble extracts containing TP, TC, PC contents, respectively	432:498	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	0	42	theme	Extraction	16:25	arg1	Evaluation					76:85	Evaluation	76:85	Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro	76:144	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	0	42	theme	Extraction	16:25	arg1	Optimization					0:11	Optimization	0:11	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds	0:70	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	5	43	theme	functional	968:977	arg1	components					979:988	functional components	968:988	functional components of oil	968:995	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	0	44	theme	Effects	129:135	arg1	Evaluation					76:85	Evaluation	76:85	Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro	76:144	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	0	44	theme	Effects	129:135	arg1	Optimization					0:11	Optimization	0:11	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds	0:70	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	4	45	theme	111.23	644:649	arg1	mg/g					658:661	111.23 ± 1.29 mg/g	644:661	111.23 ± 1.29 mg/g	644:661	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	45	theme	111.23	644:649	arg1	mg/mL					637:641	44.12 ± 0.21 mg/mL	624:641	44.12 ± 0.21 mg/mL	624:641	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	8	46	theme	high	1611:1614	arg1	value					1622:1626	high added value	1611:1626	high added value	1611:1626	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	1	47	theme	hypoglycemic	190:201	arg1	ingredients					203:213	hypoglycemic ingredients	190:213	hypoglycemic ingredients	190:213	Grape seeds full with oil and also rich in hypoglycemic ingredients.					
29668036	0	48	theme	Ingredients	43:53	arg1	Extraction					16:25	Extraction	16:25	Extraction of Hypoglycemic Ingredients from Grape Seeds	16:70	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	8	49	from	substances	1450:1459	arg1	rich					1396:1399	rich	1396:1399	rich	1396:1399	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	4	50	theme	±	706:706	arg1	g/mL					713:716	25.25 ± 0.53 g/mL	700:716	25.25 ± 0.53 g/mL	700:716	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	51	dep	GSAE	664:667	arg1	g/mL					766:769	66.68 ± 1.1 g/mL	754:769	66.68 ± 1.1 g/mL	754:769	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	51	dep	GSAE	664:667	arg1	g/mL					713:716	25.25 ± 0.53 g/mL	700:716	25.25 ± 0.53 g/mL	700:716	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	0	52	theme	Hypoglycemic	30:41	arg1	Ingredients					43:53	Hypoglycemic Ingredients	30:53	Hypoglycemic Ingredients from Grape Seeds	30:70	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	5	53	theme	oil	993:995	arg1	components					979:988	functional components	968:988	functional components of oil	968:995	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	8	54	from	anthocyanins	1404:1415	arg1	rich					1396:1399	rich	1396:1399	rich	1396:1399	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	7	55	theme	activity	1313:1320	arg1	composition					1268:1278	composition	1268:1278	composition	1268:1278	The optimized extraction, composition of constituents and hypoglycemic activity were investigated in this study.					
29668036	7	55	theme	activity	1313:1320	arg1	extraction					1256:1265	extraction	1256:1265	extraction	1256:1265	The optimized extraction, composition of constituents and hypoglycemic activity were investigated in this study.					
29668036	1	56	from	ingredients	203:213	arg1	rich					182:185	rich	182:185	rich	182:185	Grape seeds full with oil and also rich in hypoglycemic ingredients.					
29668036	3	57	theme	water	432:436	arg1	extracts					446:453	water soluble extracts	432:453	water soluble extracts containing TP, TC, PC contents, respectively	432:498	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	0	58	theme	Grape	60:64	arg1	Seeds					66:70	Grape Seeds	60:70	Grape Seeds	60:70	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	0	59	from	Seeds	66:70	arg1	Extraction					16:25	Extraction	16:25	Extraction of Hypoglycemic Ingredients from Grape Seeds	16:70	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	0	59	from	Seeds	66:70	arg1	Ingredients					43:53	Hypoglycemic Ingredients	30:53	Hypoglycemic Ingredients from Grape Seeds	30:70	Optimization of Extraction of Hypoglycemic Ingredients from Grape Seeds and Evaluation of α-Glucosidase and α-Amylase Inhibitory Effects In Vitro.					
29668036	7	60	theme	constituents	1283:1294	arg1	composition					1268:1278	composition	1268:1278	composition	1268:1278	The optimized extraction, composition of constituents and hypoglycemic activity were investigated in this study.					
29668036	7	60	theme	constituents	1283:1294	arg1	extraction					1256:1265	extraction	1256:1265	extraction	1256:1265	The optimized extraction, composition of constituents and hypoglycemic activity were investigated in this study.					
29668036	8	61	theme	other	1437:1441	arg1	substances					1450:1459	other active substances	1437:1459	other active substances	1437:1459	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	3	62	theme	soluble	336:342	arg1	±					511:511	171.1 ± 1.1	505:515	171.1 ± 1.1	505:515	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	3	62	theme	soluble	336:342	arg1	substances					351:360	Extract water soluble active substances	322:360	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively,	322:499	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	8	63	from	rich	1396:1399	arg1	polyphenols					1421:1431	polyphenols	1421:1431	polyphenols	1421:1431	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	8	63	from	rich	1396:1399	arg1	anthocyanins					1404:1415	anthocyanins	1404:1415	anthocyanins	1404:1415	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	8	63	from	rich	1396:1399	arg1	substances					1450:1459	other active substances	1437:1459	other active substances	1437:1459	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	6	64	theme	APPLICATION	1141:1151	arg1	by-products					1166:1176	by-products	1166:1176	by-products of brewing wines	1166:1193	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	6	64	theme	APPLICATION	1141:1151	arg1	seeds					1159:1163	PRACTICAL APPLICATION Grape seeds	1131:1163	PRACTICAL APPLICATION Grape seeds	1131:1163	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	6	65	from	ingredients	1229:1239	arg1	rich					1200:1203	rich	1200:1203	rich	1200:1203	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	4	66	theme	α-glucosidase	677:689	arg1	IC50					691:694	α-glucosidase IC50	677:694	α-glucosidase IC50	677:694	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	2	67	theme	oil	317:319	arg1	quality					294:300	the quality	290:300	the quality of grape seeds oil	290:319	This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil.					
29668036	2	67	theme	oil	317:319	arg1	utilization					259:269	the comprehensive utilization	241:269	the comprehensive utilization of grape seeds	241:284	This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil.					
29668036	5	68	theme	functional	1050:1059	arg1	foods					1061:1065	functional foods	1050:1065	functional foods for prevention and treatment of diabetes and its complications	1050:1128	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	6	69	theme	PRACTICAL	1131:1139	arg1	by-products					1166:1176	by-products	1166:1176	by-products of brewing wines	1166:1193	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	6	69	theme	PRACTICAL	1131:1139	arg1	seeds					1159:1163	PRACTICAL APPLICATION Grape seeds	1131:1163	PRACTICAL APPLICATION Grape seeds	1131:1163	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	8	70	dep	rich	1396:1399	arg1	inhibited					1462:1470	inhibited	1462:1470	inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value	1462:1626	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	2	71	theme	comprehensive	245:257	arg1	utilization					259:269	the comprehensive utilization	241:269	the comprehensive utilization of grape seeds	241:284	This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil.					
29668036	2	72	theme	seeds	311:315	arg1	oil					317:319	grape seeds oil	305:319	grape seeds oil	305:319	This study is to improve the comprehensive utilization of grape seeds and the quality of grape seeds oil.					
29668036	5	73	theme	complications	1116:1128	arg1	treatment					1086:1094	treatment	1086:1094	treatment	1086:1094	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	5	73	theme	complications	1116:1128	arg1	prevention					1071:1080	prevention	1071:1080	prevention	1071:1080	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	8	74	theme	active	1443:1448	arg1	substances					1450:1459	other active substances	1437:1459	other active substances	1437:1459	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	6	75	theme	other	1223:1227	arg1	ingredients					1229:1239	other ingredients	1223:1239	other ingredients	1223:1239	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	5	76	theme	fat	909:911	arg1	ingredients					913:923	the fat ingredients	905:923	the fat ingredients remained in the grape seeds	905:951	In addition, our process guarantee the fat ingredients remained in the grape seeds and not reduce functional components of oil, our study suggests that GSAE can be used to develop functional foods for prevention and treatment of diabetes and its complications.					
29668036	4	77	dep	found	564:568	arg1	mg/g					658:661	111.23 ± 1.29 mg/g	644:661	111.23 ± 1.29 mg/g	644:661	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	77	dep	found	564:568	arg1	mg/mL					637:641	44.12 ± 0.21 mg/mL	624:641	44.12 ± 0.21 mg/mL	624:641	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	4	77	dep	found	564:568	arg1	GSAE					664:667	GSAE	664:667	GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL	664:769	Also we found GSAE samples containing catechin and epicatechin were 44.12 ± 0.21 mg/mL, 111.23 ± 1.29 mg/g, GSAE against α-glucosidase IC50 was 25.25 ± 0.53 g/mL and GSAE against α-amylase IC50 was 66.68 ± 1.1 g/mL, both were competitive way, the effects of Inhibitory were obviously better than that of acarbose.					
29668036	1	78	from	rich	182:185	arg1	ingredients					203:213	hypoglycemic ingredients	190:213	hypoglycemic ingredients	190:213	Grape seeds full with oil and also rich in hypoglycemic ingredients.					
29668036	1	79	theme	Grape	147:151	arg1	seeds					153:157	Grape seeds	147:157	Grape seeds full with oil	147:171	Grape seeds full with oil and also rich in hypoglycemic ingredients.					
29668036	8	80	with	seeds	1600:1604	arg1	value					1622:1626	high added value	1611:1626	high added value	1611:1626	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29668036	3	81	theme	grape	389:393	arg1	seeds					395:399	grape seeds	389:399	grape seeds	389:399	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	6	82	from	polyphenol	1208:1217	arg1	rich					1200:1203	rich	1200:1203	rich	1200:1203	PRACTICAL APPLICATION Grape seeds, by-products of brewing wines, are rich in polyphenol and other ingredients.					
29668036	3	83	theme	extracting	369:378	arg1	oil					380:382	extracting oil	369:382	extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively,	369:499	Extract water soluble active substances before extracting oil from grape seeds, which show the grape seeds in water soluble extracts containing TP, TC, PC contents, respectively, are 171.1 ± 1.1, 658.8 ± 0.2, 482.3 ± 0.2 (mg GSAE/g).					
29668036	8	84	theme	practical	1539:1547	arg1	knowledge					1549:1557	practical knowledge	1539:1557	practical knowledge	1539:1557	The results showed that grape seeds were rich in anthocyanins and polyphenols and other active substances, inhibited α-glucosidase and α-amylase activity, which provide background and practical knowledge for the deep-processed products of grape seeds with high added value.					
29471713	0	0	link	marrow-derived	95:108	arg1	cells					127:131	rat bone marrow-derived mesenchymal stem cells	86:131	rat bone marrow-derived mesenchymal stem cells	86:131	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	6	1	theme	proliferation	1092:1104	arg1	ability					1106:1112	The proliferation ability	1088:1112	The proliferation ability	1088:1112	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	6	1	theme	proliferation	1092:1104	arg1	differentiation					1141:1155	osteogenic and adipogenic differentiation	1115:1155	osteogenic and adipogenic differentiation	1115:1155	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	2	2	theme	injury	570:575	arg1	repair					577:582	other soft tissue injury repair	552:582	other soft tissue injury repair	552:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	3	theme	Silk	379:382	arg1	coating					449:455	metal implant coating	435:455	metal implant coating for bone injury repair	435:478	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	3	theme	Silk	379:382	arg1	scaffold					512:519	tissue engineering scaffold	493:519	tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair	493:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	3	theme	Silk	379:382	arg1	film					406:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	1	4	theme	films	265:269	arg1	film					166:169	a biodegradable polymer thin film	137:169	a biodegradable polymer thin film	137:169	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	1	4	theme	films	265:269	arg1	defects					223:229	the defects	219:229	the defects of pure silk fibroin and chitosan films, respectively	219:283	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	6	5	theme	cells	1211:1215	arg1	abilities					1157:1165	The proliferation ability, osteogenic and adipogenic differentiation abilities	1088:1165	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells	1088:1215	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	8	6	theme	silk	1731:1734	arg1	film					1758:1761	silk fibroin/chitosan thin film	1731:1761	silk fibroin/chitosan thin film	1731:1761	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	9	7	theme	thin	1830:1833	arg1	material					1861:1868	a promising material	1849:1868	a promising material for tissue engineering of bone, cartilage, adipose, and skin	1849:1929	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	9	7	theme	thin	1830:1833	arg1	film					1835:1838	the silk fibroin/chitosan thin film	1804:1838	the silk fibroin/chitosan thin film	1804:1838	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	6	8	theme	mesenchymal	1194:1204	arg1	cells					1211:1215	rat bone marrow-derived mesenchymal stem cells	1170:1215	rat bone marrow-derived mesenchymal stem cells	1170:1215	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	3	9	theme	seed	698:701	arg1	cell					703:706	important seed cell	688:706	important seed cell of tissue engineering and regenerative medicine	688:754	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	6	10	theme	tissue	1302:1307	arg1	plates					1317:1322	polystyrene tissue culture plates	1290:1322	polystyrene tissue culture plates	1290:1322	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	1	11	theme	fibroin/chitosan	177:192	arg1	film					204:207	silk fibroin/chitosan composite film	172:207	silk fibroin/chitosan composite film	172:207	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	4	12	theme	solvent	835:841	arg1	method					851:856	solvent casting method	835:856	solvent casting method	835:856	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	7	13	theme	rat	1460:1462	arg1	cells					1501:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	9	14	theme	silk	1808:1811	arg1	material					1861:1868	a promising material	1849:1868	a promising material for tissue engineering of bone, cartilage, adipose, and skin	1849:1929	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	9	14	theme	silk	1808:1811	arg1	film					1835:1838	the silk fibroin/chitosan thin film	1804:1838	the silk fibroin/chitosan thin film	1804:1838	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	3	15	theme	regenerative	734:745	arg1	medicine					747:754	regenerative medicine	734:754	regenerative medicine	734:754	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	8	16	from	behavior	1655:1662	arg1	film					1694:1697	silk fibroin/chitosan thin film	1667:1697	silk fibroin/chitosan thin film	1667:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	8	17	dep	cells	1649:1653	arg1	behavior					1655:1662	behavior	1655:1662	behavior	1655:1662	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	4	18	theme	stem	903:906	arg1	cells					908:912	the rat bone marrow-derived mesenchymal stem cells	863:912	the rat bone marrow-derived mesenchymal stem cells	863:912	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	3	19	theme	engineering	718:728	arg1	cell					703:706	important seed cell	688:706	important seed cell of tissue engineering and regenerative medicine	688:754	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	4	20	theme	rat	867:869	arg1	cells					908:912	the rat bone marrow-derived mesenchymal stem cells	863:912	the rat bone marrow-derived mesenchymal stem cells	863:912	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	6	21	theme	fibroin/chitosan	1259:1274	arg1	film					1281:1284	silk fibroin/chitosan thin film	1254:1284	silk fibroin/chitosan thin film	1254:1284	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	2	22	theme	soft	558:561	arg1	repair					577:582	other soft tissue injury repair	552:582	other soft tissue injury repair	552:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	5	23	theme	mesenchymal	1037:1047	arg1	cells					1054:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively.					
29471713	4	24	theme	marrow-derived	876:889	arg1	cells					908:912	the rat bone marrow-derived mesenchymal stem cells	863:912	the rat bone marrow-derived mesenchymal stem cells	863:912	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	7	25	theme	comparable	1401:1410	arg1	environment					1412:1422	a comparable environment	1399:1422	a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells	1399:1505	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	5	26	theme	cells	1054:1058	arg1	differentiation					994:1008	Osteogenic and adipogenic differentiation	968:1008	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells	968:1058	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively.					
29471713	3	27	theme	silk	618:621	arg1	film					645:648	silk fibroin/chitosan thin film	618:648	silk fibroin/chitosan thin film for mesenchymal stem cells	618:675	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	1	28	theme	biodegradable	139:151	arg1	defects					223:229	the defects	219:229	the defects of pure silk fibroin and chitosan films, respectively	219:283	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	1	28	theme	biodegradable	139:151	arg1	film					166:169	a biodegradable polymer thin film	137:169	a biodegradable polymer thin film	137:169	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	4	29	theme	thin	957:960	arg1	film					962:965	the silk fibroin/chitosan thin film	931:965	the silk fibroin/chitosan thin film	931:965	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	2	30	theme	engineering	500:510	arg1	scaffold					512:519	tissue engineering scaffold	493:519	tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair	493:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	30	theme	engineering	500:510	arg1	film					406:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	31	theme	tissue	493:498	arg1	engineering					500:510	tissue engineering	493:510	tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair	493:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	32	theme	other	552:556	arg1	repair					577:582	other soft tissue injury repair	552:582	other soft tissue injury repair	552:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	0	33	theme	rat	86:88	arg1	cells					127:131	rat bone marrow-derived mesenchymal stem cells	86:131	rat bone marrow-derived mesenchymal stem cells	86:131	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	7	34	link	marrow-derived	1469:1482	arg1	cells					1501:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	0	35	theme	marrow-derived	95:108	arg1	cells					127:131	rat bone marrow-derived mesenchymal stem cells	86:131	rat bone marrow-derived mesenchymal stem cells	86:131	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	7	36	theme	cells	1501:1505	arg1	proliferation					1443:1455	proliferation	1443:1455	proliferation	1443:1455	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	7	36	theme	cells	1501:1505	arg1	growth					1432:1437	growth	1432:1437	growth	1432:1437	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	4	37	theme	silk	935:938	arg1	film					962:965	the silk fibroin/chitosan thin film	931:965	the silk fibroin/chitosan thin film	931:965	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	0	38	theme	stem	122:125	arg1	cells					127:131	rat bone marrow-derived mesenchymal stem cells	86:131	rat bone marrow-derived mesenchymal stem cells	86:131	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	2	39	theme	injury	466:471	arg1	repair					473:478	bone injury repair	461:478	bone injury repair	461:478	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	8	40	theme	rat	1608:1610	arg1	cells					1649:1653	rat bone marrow-derived mesenchymal stem cells	1608:1653	rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film	1608:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	0	41	theme	Silk	0:3	arg1	film					27:30	Silk fibroin/chitosan thin film	0:30	Silk fibroin/chitosan thin film	0:30	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	9	42	theme	bone	1896:1899	arg1	engineering					1881:1891	tissue engineering	1874:1891	tissue engineering of bone, cartilage, adipose, and skin	1874:1929	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	8	43	theme	marrow-derived	1617:1630	arg1	cells					1649:1653	rat bone marrow-derived mesenchymal stem cells	1608:1653	rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film	1608:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	2	44	theme	implant	441:447	arg1	coating					449:455	metal implant coating	435:455	metal implant coating for bone injury repair	435:478	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	44	theme	implant	441:447	arg1	film					406:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	0	45	theme	thin	22:25	arg1	film					27:30	Silk fibroin/chitosan thin film	0:30	Silk fibroin/chitosan thin film	0:30	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	7	46	theme	marrow-derived	1469:1482	arg1	cells					1501:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	9	47	theme	fibroin/chitosan	1813:1828	arg1	material					1861:1868	a promising material	1849:1868	a promising material for tissue engineering of bone, cartilage, adipose, and skin	1849:1929	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	9	47	theme	fibroin/chitosan	1813:1828	arg1	film					1835:1838	the silk fibroin/chitosan thin film	1804:1838	the silk fibroin/chitosan thin film	1804:1838	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	7	48	theme	fibroin/chitosan	1354:1369	arg1	film					1376:1379	silk fibroin/chitosan thin film	1349:1379	silk fibroin/chitosan thin film	1349:1379	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	9	49	theme	cartilage	1902:1910	arg1	engineering					1881:1891	tissue engineering	1874:1891	tissue engineering of bone, cartilage, adipose, and skin	1874:1929	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	8	50	theme	stem	1644:1647	arg1	cells					1649:1653	rat bone marrow-derived mesenchymal stem cells	1608:1653	rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film	1608:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	4	51	theme	silk	792:795	arg1	film					814:817	silk fibroin/chitosan film	792:817	silk fibroin/chitosan film	792:817	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	5	52	theme	rat	1013:1015	arg1	cells					1054:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively.					
29471713	9	53	theme	adipose	1913:1919	arg1	engineering					1881:1891	tissue engineering	1874:1891	tissue engineering of bone, cartilage, adipose, and skin	1874:1929	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	3	54	theme	thin	640:643	arg1	film					645:648	silk fibroin/chitosan thin film	618:648	silk fibroin/chitosan thin film for mesenchymal stem cells	618:675	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	1	55	theme	appropriate	325:335	arg1	hydrophilicity					337:350	appropriate hydrophilicity	325:350	appropriate hydrophilicity	325:350	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	5	56	theme	marrow-derived	1022:1035	arg1	cells					1054:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively.					
29471713	6	57	theme	adipogenic	1130:1139	arg1	ability					1106:1112	The proliferation ability	1088:1112	The proliferation ability	1088:1112	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	6	57	theme	adipogenic	1130:1139	arg1	differentiation					1141:1155	osteogenic and adipogenic differentiation	1115:1155	osteogenic and adipogenic differentiation	1115:1155	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	8	58	theme	silk	1667:1670	arg1	film					1694:1697	silk fibroin/chitosan thin film	1667:1697	silk fibroin/chitosan thin film	1667:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	8	59	theme	fibroin/chitosan	1736:1751	arg1	film					1758:1761	silk fibroin/chitosan thin film	1731:1761	silk fibroin/chitosan thin film	1731:1761	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	1	60	theme	pure	234:237	arg1	fibroin					244:250	pure silk fibroin	234:250	pure silk fibroin	234:250	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	7	61	theme	mesenchymal	1484:1494	arg1	cells					1501:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	1	62	theme	thin	161:164	arg1	defects					223:229	the defects	219:229	the defects of pure silk fibroin and chitosan films, respectively	219:283	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	1	62	theme	thin	161:164	arg1	film					166:169	a biodegradable polymer thin film	137:169	a biodegradable polymer thin film	137:169	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	8	63	theme	thin	1689:1692	arg1	film					1694:1697	silk fibroin/chitosan thin film	1667:1697	silk fibroin/chitosan thin film	1667:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	3	64	theme	stem	666:669	arg1	cells					671:675	mesenchymal stem cells	654:675	mesenchymal stem cells	654:675	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	8	65	theme	film	1758:1761	arg1	application					1716:1726	the application	1712:1726	the application of silk fibroin/chitosan thin film	1712:1761	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	1	66	theme	fibroin	244:250	arg1	film					166:169	a biodegradable polymer thin film	137:169	a biodegradable polymer thin film	137:169	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	1	66	theme	fibroin	244:250	arg1	defects					223:229	the defects	219:229	the defects of pure silk fibroin and chitosan films, respectively	219:283	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	7	67	theme	adipogenic	1546:1555	arg1	differentiation					1557:1571	their osteogenic and adipogenic differentiation	1525:1571	their osteogenic and adipogenic differentiation	1525:1571	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	6	68	theme	ability	1106:1112	arg1	abilities					1157:1165	The proliferation ability, osteogenic and adipogenic differentiation abilities	1088:1165	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells	1088:1215	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	3	69	theme	important	688:696	arg1	cell					703:706	important seed cell	688:706	important seed cell of tissue engineering and regenerative medicine	688:754	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	2	70	theme	fibroin/chitosan	384:399	arg1	coating					449:455	metal implant coating	435:455	metal implant coating for bone injury repair	435:478	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	70	theme	fibroin/chitosan	384:399	arg1	scaffold					512:519	tissue engineering scaffold	493:519	tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair	493:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	70	theme	fibroin/chitosan	384:399	arg1	film					406:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	1	71	theme	chitosan	256:263	arg1	films					265:269	chitosan films	256:269	chitosan films	256:269	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	7	72	theme	osteogenic	1531:1540	arg1	differentiation					1557:1571	their osteogenic and adipogenic differentiation	1525:1571	their osteogenic and adipogenic differentiation	1525:1571	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	4	73	theme	casting	843:849	arg1	method					851:856	solvent casting method	835:856	solvent casting method	835:856	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	4	74	link	marrow-derived	876:889	arg1	cells					908:912	the rat bone marrow-derived mesenchymal stem cells	863:912	the rat bone marrow-derived mesenchymal stem cells	863:912	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	6	75	theme	stem	1206:1209	arg1	cells					1211:1215	rat bone marrow-derived mesenchymal stem cells	1170:1215	rat bone marrow-derived mesenchymal stem cells	1170:1215	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	3	76	theme	tissue	711:716	arg1	engineering					718:728	tissue engineering	711:728	tissue engineering	711:728	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	6	77	theme	culture	1309:1315	arg1	plates					1317:1322	polystyrene tissue culture plates	1290:1322	polystyrene tissue culture plates	1290:1322	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	1	78	theme	silk	172:175	arg1	film					204:207	silk fibroin/chitosan composite film	172:207	silk fibroin/chitosan composite film	172:207	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	6	79	theme	marrow-derived	1179:1192	arg1	cells					1211:1215	rat bone marrow-derived mesenchymal stem cells	1170:1215	rat bone marrow-derived mesenchymal stem cells	1170:1215	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	3	80	theme	cell	703:706	arg1	biocompatibility					598:613	the biocompatibility	594:613	the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells	594:675	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	3	80	theme	cell	703:706	arg1	kind					680:683	a kind	678:683	a kind of important seed cell of tissue engineering and regenerative medicine	678:754	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	6	81	theme	polystyrene	1290:1300	arg1	plates					1317:1322	polystyrene tissue culture plates	1290:1322	polystyrene tissue culture plates	1290:1322	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	1	82	theme	composite	194:202	arg1	film					204:207	silk fibroin/chitosan composite film	172:207	silk fibroin/chitosan composite film	172:207	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	7	83	theme	stem	1496:1499	arg1	cells					1501:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	7	84	theme	bone	1464:1467	arg1	cells					1501:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	rat bone marrow-derived mesenchymal stem cells	1460:1505	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	3	85	theme	medicine	747:754	arg1	cell					703:706	important seed cell	688:706	important seed cell of tissue engineering and regenerative medicine	688:754	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	4	86	theme	mesenchymal	891:901	arg1	cells					908:912	the rat bone marrow-derived mesenchymal stem cells	863:912	the rat bone marrow-derived mesenchymal stem cells	863:912	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	5	87	link	marrow-derived	1022:1035	arg1	cells					1054:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively.					
29471713	6	88	theme	thin	1276:1279	arg1	film					1281:1284	silk fibroin/chitosan thin film	1254:1284	silk fibroin/chitosan thin film	1254:1284	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	9	89	theme	promising	1851:1859	arg1	material					1861:1868	a promising material	1849:1868	a promising material for tissue engineering of bone, cartilage, adipose, and skin	1849:1929	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	9	89	theme	promising	1851:1859	arg1	film					1835:1838	the silk fibroin/chitosan thin film	1804:1838	the silk fibroin/chitosan thin film	1804:1838	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	2	90	theme	tissue	563:568	arg1	repair					577:582	other soft tissue injury repair	552:582	other soft tissue injury repair	552:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	6	91	theme	silk	1254:1257	arg1	film					1281:1284	silk fibroin/chitosan thin film	1254:1284	silk fibroin/chitosan thin film	1254:1284	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	5	92	theme	stem	1049:1052	arg1	cells					1054:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively.					
29471713	4	93	theme	bone	871:874	arg1	cells					908:912	the rat bone marrow-derived mesenchymal stem cells	863:912	the rat bone marrow-derived mesenchymal stem cells	863:912	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	6	94	link	marrow-derived	1179:1192	arg1	cells					1211:1215	rat bone marrow-derived mesenchymal stem cells	1170:1215	rat bone marrow-derived mesenchymal stem cells	1170:1215	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	1	95	theme	polymer	153:159	arg1	defects					223:229	the defects	219:229	the defects of pure silk fibroin and chitosan films, respectively	219:283	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	1	95	theme	polymer	153:159	arg1	film					166:169	a biodegradable polymer thin film	137:169	a biodegradable polymer thin film	137:169	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	4	96	theme	fibroin/chitosan	940:955	arg1	film					962:965	the silk fibroin/chitosan thin film	931:965	the silk fibroin/chitosan thin film	931:965	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	2	97	used	used	418:421	arg2	film					406:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	97	used	used	418:421	arg2	coating					449:455	metal implant coating	435:455	metal implant coating for bone injury repair	435:478	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	97	used	used	418:421	arg2	scaffold					512:519	tissue engineering scaffold	493:519	tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair	493:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	0	98	theme	bone	90:93	arg1	cells					127:131	rat bone marrow-derived mesenchymal stem cells	86:131	rat bone marrow-derived mesenchymal stem cells	86:131	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	1	99	theme	mechanical	356:365	arg1	properties					367:376	mechanical properties	356:376	mechanical properties	356:376	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	0	100	theme	mesenchymal	110:120	arg1	cells					127:131	rat bone marrow-derived mesenchymal stem cells	86:131	rat bone marrow-derived mesenchymal stem cells	86:131	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	6	101	theme	osteogenic	1115:1124	arg1	ability					1106:1112	The proliferation ability	1088:1112	The proliferation ability	1088:1112	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	6	101	theme	osteogenic	1115:1124	arg1	differentiation					1141:1155	osteogenic and adipogenic differentiation	1115:1155	osteogenic and adipogenic differentiation	1115:1155	The proliferation ability, osteogenic and adipogenic differentiation abilities of rat bone marrow-derived mesenchymal stem cells were systematically compared between silk fibroin/chitosan thin film and polystyrene tissue culture plates.					
29471713	0	102	theme	cells	127:131	arg1	differentiation					67:81	osteogenic and adipogenic differentiation	41:81	osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells	41:131	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	9	103	theme	tissue	1874:1879	arg1	engineering					1881:1891	tissue engineering	1874:1891	tissue engineering of bone, cartilage, adipose, and skin	1874:1929	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	8	104	theme	bone	1612:1615	arg1	cells					1649:1653	rat bone marrow-derived mesenchymal stem cells	1608:1653	rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film	1608:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	0	105	theme	fibroin/chitosan	5:20	arg1	film					27:30	Silk fibroin/chitosan thin film	0:30	Silk fibroin/chitosan thin film	0:30	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	7	106	theme	thin	1371:1374	arg1	film					1376:1379	silk fibroin/chitosan thin film	1349:1379	silk fibroin/chitosan thin film	1349:1379	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	2	107	theme	bone	461:464	arg1	repair					473:478	bone injury repair	461:478	bone injury repair	461:478	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	8	108	theme	mesenchymal	1632:1642	arg1	cells					1649:1653	rat bone marrow-derived mesenchymal stem cells	1608:1653	rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film	1608:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	7	109	theme	silk	1349:1352	arg1	film					1376:1379	silk fibroin/chitosan thin film	1349:1379	silk fibroin/chitosan thin film	1349:1379	The results showed that silk fibroin/chitosan thin film not only provided a comparable environment for the growth and proliferation of rat bone marrow-derived mesenchymal stem cells but also promoted their osteogenic and adipogenic differentiation.					
29471713	8	110	theme	cells	1649:1653	arg1	information					1593:1603	information	1593:1603	information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film	1593:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	3	111	theme	film	645:648	arg1	biocompatibility					598:613	the biocompatibility	594:613	the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells	594:675	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	3	111	theme	film	645:648	arg1	kind					680:683	a kind	678:683	a kind of important seed cell of tissue engineering and regenerative medicine	678:754	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	2	112	theme	metal	435:439	arg1	coating					449:455	metal implant coating	435:455	metal implant coating for bone injury repair	435:478	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	112	theme	metal	435:439	arg1	film					406:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	0	113	theme	osteogenic	41:50	arg1	differentiation					67:81	osteogenic and adipogenic differentiation	41:81	osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells	41:131	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	1	114	theme	remarkable	296:305	arg1	biocompatibility					307:322	remarkable biocompatibility	296:322	remarkable biocompatibility	296:322	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	3	115	theme	fibroin/chitosan	623:638	arg1	film					645:648	silk fibroin/chitosan thin film	618:648	silk fibroin/chitosan thin film for mesenchymal stem cells	618:675	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	8	116	link	marrow-derived	1617:1630	arg1	cells					1649:1653	rat bone marrow-derived mesenchymal stem cells	1608:1653	rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film	1608:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	0	117	theme	adipogenic	56:65	arg1	differentiation					67:81	osteogenic and adipogenic differentiation	41:81	osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells	41:131	Silk fibroin/chitosan thin film promotes osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells.					
29471713	5	118	theme	bone	1017:1020	arg1	cells					1054:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	rat bone marrow-derived mesenchymal stem cells	1013:1058	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively.					
29471713	8	119	theme	fibroin/chitosan	1672:1687	arg1	film					1694:1697	silk fibroin/chitosan thin film	1667:1697	silk fibroin/chitosan thin film	1667:1697	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	4	120	theme	fibroin/chitosan	797:812	arg1	film					814:817	silk fibroin/chitosan film	792:817	silk fibroin/chitosan film	792:817	In this study, silk fibroin/chitosan film was prepared by solvent casting method, and the rat bone marrow-derived mesenchymal stem cells were cultured on the silk fibroin/chitosan thin film.					
29471713	8	121	theme	thin	1753:1756	arg1	film					1758:1761	silk fibroin/chitosan thin film	1731:1761	silk fibroin/chitosan thin film	1731:1761	This work provided information of rat bone marrow-derived mesenchymal stem cells behavior on silk fibroin/chitosan thin film and extended the application of silk fibroin/chitosan thin film.					
29471713	5	122	theme	Osteogenic	968:977	arg1	differentiation					994:1008	Osteogenic and adipogenic differentiation	968:1008	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells	968:1058	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively.					
29471713	9	123	theme	skin	1926:1929	arg1	engineering					1881:1891	tissue engineering	1874:1891	tissue engineering of bone, cartilage, adipose, and skin	1874:1929	Based on the results, we suggested that the silk fibroin/chitosan thin film could be a promising material for tissue engineering of bone, cartilage, adipose, and skin.					
29471713	3	124	theme	mesenchymal	654:664	arg1	cells					671:675	mesenchymal stem cells	654:675	mesenchymal stem cells	654:675	However, the biocompatibility of silk fibroin/chitosan thin film for mesenchymal stem cells, a kind of important seed cell of tissue engineering and regenerative medicine, is rarely reported.					
29471713	1	125	theme	silk	239:242	arg1	fibroin					244:250	pure silk fibroin	234:250	pure silk fibroin	234:250	As a biodegradable polymer thin film, silk fibroin/chitosan composite film overcomes the defects of pure silk fibroin and chitosan films, respectively, and shows remarkable biocompatibility, appropriate hydrophilicity and mechanical properties.					
29471713	5	126	theme	adipogenic	983:992	arg1	differentiation					994:1008	Osteogenic and adipogenic differentiation	968:1008	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells	968:1058	Osteogenic and adipogenic differentiation of rat bone marrow-derived mesenchymal stem cells were induced, respectively.					
29471713	2	127	theme	thin	401:404	arg1	coating					449:455	metal implant coating	435:455	metal implant coating for bone injury repair	435:478	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	127	theme	thin	401:404	arg1	scaffold					512:519	tissue engineering scaffold	493:519	tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair	493:582	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29471713	2	127	theme	thin	401:404	arg1	film					406:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film	379:409	Silk fibroin/chitosan thin film can be used not only as metal implant coating for bone injury repair, but also as tissue engineering scaffold for skin, cornea, adipose, and other soft tissue injury repair.					
29943499	0	0	theme	Dual-Stage	102:111	arg1	Crosslinking					113:124	Alginate-Templated Dual-Stage Crosslinking	83:124	Alginate-Templated Dual-Stage Crosslinking	83:124	A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking.					
29943499	7	1	theme	physical	1331:1338	arg1	crosslinking					1340:1351	subsequent chemical or physical crosslinking	1308:1351	subsequent chemical or physical crosslinking of the bio-macromolecular component	1308:1387	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	8	2	theme	variety	1566:1572	arg1	use					1552:1554	use	1552:1554	use of a wide variety of other bio-macromolecular bioinks	1552:1608	It is anticipated that this strategy is general, and can be readily expanded for use of a wide variety of other bio-macromolecular bioinks.					
29943499	2	3	theme	successful	390:399	arg1	bioprinting					401:411	successful bioprinting	390:411	successful bioprinting	390:411	The selection of proper biomaterials as the bioinks is a key step toward successful bioprinting.					
29943499	6	4	theme	desired	1085:1091	arg1	component					1112:1120	the desired bio-macromolecular component	1081:1120	the desired bio-macromolecular component	1081:1120	Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate.					
29943499	0	5	theme	Alginate-Templated	83:100	arg1	Crosslinking					113:124	Alginate-Templated Dual-Stage Crosslinking	83:124	Alginate-Templated Dual-Stage Crosslinking	83:124	A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking.					
29943499	7	6	theme	component	1379:1387	arg1	crosslinking					1340:1351	subsequent chemical or physical crosslinking	1308:1351	subsequent chemical or physical crosslinking of the bio-macromolecular component	1308:1387	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	1	7	theme	human	297:301	arg1	counterparts					303:314	their human counterparts	291:314	their human counterparts	291:314	The recently developed 3D bioprinting technology has greatly improved the ability to generate biomimetic tissues that are structurally and functionally relevant to their human counterparts.					
29943499	7	8	theme	chemical	1319:1326	arg1	crosslinking					1340:1351	subsequent chemical or physical crosslinking	1308:1351	subsequent chemical or physical crosslinking of the bio-macromolecular component	1308:1387	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	7	9	theme	structural	1224:1233	arg1	support					1235:1241	the temporal structural support	1211:1241	the temporal structural support	1211:1241	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	7	9	theme	structural	1224:1233	arg1	component					1191:1199	The physically crosslinkable alginate component	1153:1199	The physically crosslinkable alginate component	1153:1199	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	2	10	theme	proper	334:339	arg1	biomaterials					341:352	proper biomaterials	334:352	proper biomaterials	334:352	The selection of proper biomaterials as the bioinks is a key step toward successful bioprinting.					
29943499	3	11	theme	structures	547:556	arg1	deposition					519:528	deposition	519:528	deposition of free-standing structures	519:556	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	3	11	theme	structures	547:556	arg1	maintenance					566:576	the maintenance	562:576	the maintenance of architectural integrity	562:603	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	4	12	theme	mechanical	766:775	arg1	properties					777:786	their commonly low mechanical properties	747:786	their commonly low mechanical properties	747:786	This requirement, however, has greatly limited the selection of bioinks, especially for those naturally derived due to their commonly low mechanical properties.					
29943499	3	13	theme	rheological	465:475	arg1	parameter					477:485	an important rheological parameter	452:485	an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting	452:625	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	3	13	theme	rheological	465:475	arg1	viscosity					427:435	viscosity	427:435	viscosity of a bioink	427:447	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	7	14	theme	subsequent	1308:1317	arg1	crosslinking					1340:1351	subsequent chemical or physical crosslinking	1308:1351	subsequent chemical or physical crosslinking of the bio-macromolecular component	1308:1387	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	7	15	theme	temporal	1215:1222	arg1	support					1235:1241	the temporal structural support	1211:1241	the temporal structural support	1211:1241	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	7	15	theme	temporal	1215:1222	arg1	component					1191:1199	The physically crosslinkable alginate component	1153:1199	The physically crosslinkable alginate component	1153:1199	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	3	16	theme	architectural	581:593	arg1	integrity					595:603	architectural integrity	581:603	architectural integrity	581:603	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	0	17	theme	General	2:8	arg1	Strategy					10:17	A General Strategy	0:17	A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking.	0:125	A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking.					
29943499	5	18	theme	extrusion	832:840	arg1	bioprinting					842:852	extrusion bioprinting	832:852	extrusion bioprinting of bio-macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl,	832:978	Here the generalization of a mechanism for extrusion bioprinting of bio-macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl, is reported.					
29943499	6	19	contain	containing	1065:1074	arg2	component					1112:1120	the desired bio-macromolecular component	1081:1120	the desired bio-macromolecular component	1081:1120	Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate.					
29943499	6	19	contain	containing	1065:1074	arg2	alginate					1143:1150	a polysaccharide alginate	1126:1150	a polysaccharide alginate	1126:1150	Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate.					
29943499	6	19	contain	containing	1065:1074	arg1	bioink					1058:1063	a composite bioink	1046:1063	a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate	1046:1150	Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate.					
29943499	7	20	theme	desired	1444:1450	arg1	bio-macromolecule					1452:1468	only the desired bio-macromolecule	1435:1468	only the desired bio-macromolecule	1435:1468	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	7	21	theme	bio-macromolecular	1360:1377	arg1	component					1379:1387	the bio-macromolecular component	1356:1387	the bio-macromolecular component	1356:1387	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	3	22	theme	important	455:463	arg1	parameter					477:485	an important rheological parameter	452:485	an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting	452:625	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	3	22	theme	important	455:463	arg1	viscosity					427:435	viscosity	427:435	viscosity of a bioink	427:447	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	7	23	theme	construct	1273:1281	arg1	shape					1260:1264	the shape	1256:1264	the shape of the construct	1256:1281	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	6	24	theme	composite	1048:1056	arg1	bioink					1058:1063	a composite bioink	1046:1063	a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate	1046:1150	Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate.					
29943499	7	25	theme	alginate	1182:1189	arg1	support					1235:1241	the temporal structural support	1211:1241	the temporal structural support	1211:1241	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	7	25	theme	alginate	1182:1189	arg1	component					1191:1199	The physically crosslinkable alginate component	1153:1199	The physically crosslinkable alginate component	1153:1199	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	5	26	theme	bio-macromolecular	857:874	arg1	components					876:885	bio-macromolecular components	857:885	bio-macromolecular components	857:885	Here the generalization of a mechanism for extrusion bioprinting of bio-macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl, is reported.					
29943499	4	27	theme	bioinks	692:698	arg1	selection					679:687	the selection	675:687	the selection of bioinks, especially for those naturally derived due to their commonly low mechanical properties	675:786	This requirement, however, has greatly limited the selection of bioinks, especially for those naturally derived due to their commonly low mechanical properties.					
29943499	8	28	theme	wide	1561:1564	arg1	variety					1566:1572	a wide variety	1559:1572	a wide variety of other bio-macromolecular bioinks	1559:1608	It is anticipated that this strategy is general, and can be readily expanded for use of a wide variety of other bio-macromolecular bioinks.					
29943499	8	28	theme	wide	1561:1564	arg1	bioinks					1602:1608	other bio-macromolecular bioinks	1577:1608	other bio-macromolecular bioinks	1577:1608	It is anticipated that this strategy is general, and can be readily expanded for use of a wide variety of other bio-macromolecular bioinks.					
29943499	2	29	theme	biomaterials	341:352	arg1	step					378:381	a key step	372:381	a key step toward successful bioprinting	372:411	The selection of proper biomaterials as the bioinks is a key step toward successful bioprinting.					
29943499	2	29	theme	biomaterials	341:352	arg1	selection					321:329	The selection	317:329	The selection of proper biomaterials as the bioinks	317:367	The selection of proper biomaterials as the bioinks is a key step toward successful bioprinting.					
29943499	3	30	theme	bioink	442:447	arg1	parameter					477:485	an important rheological parameter	452:485	an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting	452:625	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	3	30	theme	bioink	442:447	arg1	viscosity					427:435	viscosity	427:435	viscosity of a bioink	427:447	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	2	31	theme	key	374:376	arg1	step					378:381	a key step	372:381	a key step toward successful bioprinting	372:411	The selection of proper biomaterials as the bioinks is a key step toward successful bioprinting.					
29943499	2	31	theme	key	374:376	arg1	selection					321:329	The selection	317:329	The selection of proper biomaterials as the bioinks	317:367	The selection of proper biomaterials as the bioinks is a key step toward successful bioprinting.					
29943499	1	32	theme	biomimetic	221:230	arg1	tissues					232:238	biomimetic tissues	221:238	biomimetic tissues that are structurally and functionally relevant to their human counterparts	221:314	The recently developed 3D bioprinting technology has greatly improved the ability to generate biomimetic tissues that are structurally and functionally relevant to their human counterparts.					
29943499	0	33	theme	Extrusion	23:31	arg1	Bioprinting					33:43	Extrusion Bioprinting	23:43	Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking	23:124	A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking.					
29943499	7	34	theme	crosslinkable	1168:1180	arg1	support					1235:1241	the temporal structural support	1211:1241	the temporal structural support	1211:1241	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	7	34	theme	crosslinkable	1168:1180	arg1	component					1191:1199	The physically crosslinkable alginate component	1153:1199	The physically crosslinkable alginate component	1153:1199	The physically crosslinkable alginate component serves as the temporal structural support to stabilize the shape of the construct during bioprinting; upon subsequent chemical or physical crosslinking of the bio-macromolecular component, alginate can be selectively removed to leave only the desired bio-macromolecule.					
29943499	6	35	theme	polysaccharide	1128:1141	arg1	alginate					1143:1150	a polysaccharide alginate	1126:1150	a polysaccharide alginate	1126:1150	Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate.					
29943499	0	36	theme	Bio-Macromolecular	48:65	arg1	Bioinks					67:73	Bio-Macromolecular Bioinks	48:73	Bio-Macromolecular Bioinks	48:73	A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking.					
29943499	5	37	theme	mechanism	818:826	arg1	generalization					798:811	the generalization	794:811	the generalization of a mechanism for extrusion bioprinting of bio-macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl,	794:978	Here the generalization of a mechanism for extrusion bioprinting of bio-macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl, is reported.					
29943499	1	38	theme	developed	140:148	arg1	technology					165:174	The recently developed 3D bioprinting technology	127:174	The recently developed 3D bioprinting technology	127:174	The recently developed 3D bioprinting technology has greatly improved the ability to generate biomimetic tissues that are structurally and functionally relevant to their human counterparts.					
29943499	5	39	theme	components	876:885	arg1	bioprinting					842:852	extrusion bioprinting	832:852	extrusion bioprinting of bio-macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl,	832:978	Here the generalization of a mechanism for extrusion bioprinting of bio-macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl, is reported.					
29943499	8	40	theme	bio-macromolecular	1583:1600	arg1	bioinks					1602:1608	other bio-macromolecular bioinks	1577:1608	other bio-macromolecular bioinks	1577:1608	It is anticipated that this strategy is general, and can be readily expanded for use of a wide variety of other bio-macromolecular bioinks.					
29943499	5	41	dep	gelatin	946:952	arg1	methacryloyl					966:977	methacryloyl	966:977	methacryloyl	966:977	Here the generalization of a mechanism for extrusion bioprinting of bio-macromolecular components, mainly focusing on collagen and its derivatives including gelatin and gelatin methacryloyl, is reported.					
29943499	1	42	theme	3D	150:151	arg1	technology					165:174	The recently developed 3D bioprinting technology	127:174	The recently developed 3D bioprinting technology	127:174	The recently developed 3D bioprinting technology has greatly improved the ability to generate biomimetic tissues that are structurally and functionally relevant to their human counterparts.					
29943499	6	43	theme	templating	1009:1018	arg1	strategy					1020:1027	a templating strategy	1007:1027	a templating strategy	1007:1027	Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate.					
29943499	8	44	theme	other	1577:1581	arg1	bioinks					1602:1608	other bio-macromolecular bioinks	1577:1608	other bio-macromolecular bioinks	1577:1608	It is anticipated that this strategy is general, and can be readily expanded for use of a wide variety of other bio-macromolecular bioinks.					
29943499	3	45	from	flexibility	504:514	arg1	deposition					519:528	deposition	519:528	deposition of free-standing structures	519:556	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	3	45	from	flexibility	504:514	arg1	maintenance					566:576	the maintenance	562:576	the maintenance of architectural integrity	562:603	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	1	46	theme	bioprinting	153:163	arg1	technology					165:174	The recently developed 3D bioprinting technology	127:174	The recently developed 3D bioprinting technology	127:174	The recently developed 3D bioprinting technology has greatly improved the ability to generate biomimetic tissues that are structurally and functionally relevant to their human counterparts.					
29943499	3	47	theme	integrity	595:603	arg1	deposition					519:528	deposition	519:528	deposition of free-standing structures	519:556	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	3	47	theme	integrity	595:603	arg1	maintenance					566:576	the maintenance	562:576	the maintenance of architectural integrity	562:603	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	8	48	theme	bioinks	1602:1608	arg1	variety					1566:1572	a wide variety	1559:1572	a wide variety of other bio-macromolecular bioinks	1559:1608	It is anticipated that this strategy is general, and can be readily expanded for use of a wide variety of other bio-macromolecular bioinks.					
29943499	8	48	theme	bioinks	1602:1608	arg1	bioinks					1602:1608	other bio-macromolecular bioinks	1577:1608	other bio-macromolecular bioinks	1577:1608	It is anticipated that this strategy is general, and can be readily expanded for use of a wide variety of other bio-macromolecular bioinks.					
29943499	0	49	theme	Bioinks	67:73	arg1	Bioprinting					33:43	Extrusion Bioprinting	23:43	Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking	23:124	A General Strategy for Extrusion Bioprinting of Bio-Macromolecular Bioinks through Alginate-Templated Dual-Stage Crosslinking.					
29943499	6	50	theme	bio-macromolecular	1093:1110	arg1	component					1112:1120	the desired bio-macromolecular component	1081:1120	the desired bio-macromolecular component	1081:1120	Specifically, a templating strategy is adopted using a composite bioink containing both the desired bio-macromolecular component and a polysaccharide alginate.					
29943499	3	51	theme	free-standing	533:545	arg1	structures					547:556	free-standing structures	533:556	free-standing structures	533:556	For example, viscosity of a bioink is an important rheological parameter to determine the flexibility in deposition of free-standing structures and the maintenance of architectural integrity following bioprinting.					
29943499	4	52	theme	low	762:764	arg1	properties					777:786	their commonly low mechanical properties	747:786	their commonly low mechanical properties	747:786	This requirement, however, has greatly limited the selection of bioinks, especially for those naturally derived due to their commonly low mechanical properties.					
31924036	6	0	theme	Fluoroquinolone	987:1001	arg1	forms					978:982	the forms	974:982	the forms of Fluoroquinolone and Cephalosporin	974:1019	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	7	1	theme	contact	1185:1191	arg1	time					1193:1196	contact time	1185:1196	contact time	1185:1196	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	14	2	theme	LEVO	2212:2215	arg1	extraction					2198:2207	extraction	2198:2207	extraction of LEVO and CFT from industrial wastewater	2198:2250	The results proved that the designed NBent-NTiO2-Chit was successively implemented for extraction of LEVO and CFT from industrial wastewater providing percentage values 83.2 and 79.0% using 10.0 and 150.0 ± 1.0 mg NBent-NTiO2-Chit, respectively.					
31924036	8	3	theme	Maximum	1220:1226	arg1	values					1239:1244	Maximum percentage values	1220:1244	Maximum percentage values	1220:1244	Maximum percentage values of 90.2% (pH 4) and 93.5% (pH 5) for LEVO and CFT, respectively, were achieved at 10 min.					
31924036	13	4	theme	R2	2050:2051	arg1	values					2053:2058	the acquired R2 values	2037:2058	the acquired R2 values	2037:2058	The kinetic studies of NBent-NTiO2-Chit with LEVO and CFT antibiotics were better fitted by the pseudo-second-order based on the acquired R2 values as 0.999 and 0.997 for LEVO and CFT, respectively.					
31924036	4	5	theme	Si-O-Al	648:654	arg1	groups					679:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	The FT-IR of NBent-NTiO2-Chit confirmed the presence of OH, N-H, Si-O-Al and Si-O-Si functional groups.					
31924036	0	6	theme	assembled	78:86	arg1	composite					88:96	assembled composite	78:96	assembled composite of nanotitanium oxide/chitosan/nano-bentonite	78:142	Enhanced adsorption of Levofloxacin and Ceftriaxone antibiotics from water by assembled composite of nanotitanium oxide/chitosan/nano-bentonite.					
31924036	0	6	theme	assembled	78:86	arg1	oxide/chitosan/nano-bentonite					114:142	nanotitanium oxide/chitosan/nano-bentonite	101:142	nanotitanium oxide/chitosan/nano-bentonite	101:142	Enhanced adsorption of Levofloxacin and Ceftriaxone antibiotics from water by assembled composite of nanotitanium oxide/chitosan/nano-bentonite.					
31924036	11	7	theme	adsorption	1762:1771	arg1	processes					1773:1781	the adsorption processes	1758:1781	the adsorption processes	1758:1781	The obtained correlation coefficients (R2) by Langmuir model were 0.952 and 0.987 for LEVO and CFT, respectively to offer excellent fit to the adsorption processes.					
31924036	6	8	theme	LEVO	947:950	arg1	CFT					966:968	CFT	966:968	CFT	966:968	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	6	8	theme	LEVO	947:950	arg1	Ceftriaxone					953:963	Levofloxacin (LEVO) Ceftriaxone	933:963	Levofloxacin (LEVO) Ceftriaxone (CFT)	933:969	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	0	9	theme	nanotitanium	101:112	arg1	oxide/chitosan/nano-bentonite					114:142	nanotitanium oxide/chitosan/nano-bentonite	101:142	nanotitanium oxide/chitosan/nano-bentonite	101:142	Enhanced adsorption of Levofloxacin and Ceftriaxone antibiotics from water by assembled composite of nanotitanium oxide/chitosan/nano-bentonite.					
31924036	7	10	theme	CFT	1180:1182	arg1	ions					1214:1217	interfering ions	1202:1217	interfering ions	1202:1217	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	7	10	theme	CFT	1180:1182	arg1	dosage					1138:1143	dosage	1138:1143	dosage	1138:1143	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	7	10	theme	CFT	1180:1182	arg1	concentration					1154:1166	initial concentration	1146:1166	initial concentration of LEVO and CFT	1146:1182	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	7	10	theme	CFT	1180:1182	arg1	pH					1134:1135	pH	1134:1135	pH	1134:1135	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	7	10	theme	CFT	1180:1182	arg1	time					1193:1196	contact time	1185:1196	contact time	1185:1196	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	6	11	theme	classes	900:906	arg1	removal					875:881	the removal	871:881	the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT)	871:969	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	4	12	theme	Si-O-Si	660:666	arg1	groups					679:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	The FT-IR of NBent-NTiO2-Chit confirmed the presence of OH, N-H, Si-O-Al and Si-O-Si functional groups.					
31924036	5	13	theme	temperature	805:815	arg1	range					817:821	the temperature range 30-600 °C	801:831	the temperature range 30-600 °C	801:831	Four thermal degradation steps were characterized from the TGA of NBent-NTiO2-Chit with a total loss = 23.514% in the temperature range 30-600 °C.					
31924036	11	14	theme	Langmuir	1665:1672	arg1	model					1674:1678	Langmuir model	1665:1678	Langmuir model	1665:1678	The obtained correlation coefficients (R2) by Langmuir model were 0.952 and 0.987 for LEVO and CFT, respectively to offer excellent fit to the adsorption processes.					
31924036	12	15	theme	thermodynamics	1788:1801	arg1	parameters					1803:1812	The thermodynamics parameters	1784:1812	The thermodynamics parameters of NBent-NTiO2-Chit	1784:1832	The thermodynamics parameters of NBent-NTiO2-Chit were evaluated and referred that the reaction is spontaneous and endothermic.					
31924036	6	16	theme	important	890:898	arg1	classes					900:906	two important classes	886:906	two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT)	886:969	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	14	17	theme	percentage	2262:2271	arg1	values					2273:2278	percentage values	2262:2278	percentage values 83.2 and 79.0%	2262:2293	The results proved that the designed NBent-NTiO2-Chit was successively implemented for extraction of LEVO and CFT from industrial wastewater providing percentage values 83.2 and 79.0% using 10.0 and 150.0 ± 1.0 mg NBent-NTiO2-Chit, respectively.					
31924036	14	18	dep	values	2273:2278	arg1	%					2293:2293	83.2 and 79.0%	2280:2293	percentage values 83.2 and 79.0%	2262:2293	The results proved that the designed NBent-NTiO2-Chit was successively implemented for extraction of LEVO and CFT from industrial wastewater providing percentage values 83.2 and 79.0% using 10.0 and 150.0 ± 1.0 mg NBent-NTiO2-Chit, respectively.					
31924036	10	19	theme	adsorption	1556:1565	arg1	isotherm					1567:1574	adsorption isotherm	1556:1574	adsorption isotherm including Langmuir	1556:1593	The extraction mechanisms of LEVO and CFT were positively explained by three models of adsorption isotherm including Langmuir, Freundlich and Temkin.					
31924036	13	20	with	studies	1924:1930	arg1	LEVO					1957:1960	LEVO	1957:1960	LEVO	1957:1960	The kinetic studies of NBent-NTiO2-Chit with LEVO and CFT antibiotics were better fitted by the pseudo-second-order based on the acquired R2 values as 0.999 and 0.997 for LEVO and CFT, respectively.					
31924036	13	20	with	studies	1924:1930	arg1	CFT					1966:1968	CFT	1966:1968	CFT	1966:1968	The kinetic studies of NBent-NTiO2-Chit with LEVO and CFT antibiotics were better fitted by the pseudo-second-order based on the acquired R2 values as 0.999 and 0.997 for LEVO and CFT, respectively.					
31924036	5	21	theme	thermal	692:698	arg1	steps					712:716	Four thermal degradation steps	687:716	Four thermal degradation steps	687:716	Four thermal degradation steps were characterized from the TGA of NBent-NTiO2-Chit with a total loss = 23.514% in the temperature range 30-600 °C.					
31924036	7	22	theme	various	1040:1046	arg1	factors					1048:1054	The various factors	1036:1054	The various factors that affected the percentage of extraction	1036:1097	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	14	23	theme	industrial	2230:2239	arg1	wastewater					2241:2250	industrial wastewater	2230:2250	industrial wastewater	2230:2250	The results proved that the designed NBent-NTiO2-Chit was successively implemented for extraction of LEVO and CFT from industrial wastewater providing percentage values 83.2 and 79.0% using 10.0 and 150.0 ± 1.0 mg NBent-NTiO2-Chit, respectively.					
31924036	14	24	from	wastewater	2241:2250	arg1	extraction					2198:2207	extraction	2198:2207	extraction of LEVO and CFT from industrial wastewater	2198:2250	The results proved that the designed NBent-NTiO2-Chit was successively implemented for extraction of LEVO and CFT from industrial wastewater providing percentage values 83.2 and 79.0% using 10.0 and 150.0 ± 1.0 mg NBent-NTiO2-Chit, respectively.					
31924036	0	25	theme	oxide/chitosan/nano-bentonite	114:142	arg1	composite					88:96	assembled composite	78:96	assembled composite of nanotitanium oxide/chitosan/nano-bentonite	78:142	Enhanced adsorption of Levofloxacin and Ceftriaxone antibiotics from water by assembled composite of nanotitanium oxide/chitosan/nano-bentonite.					
31924036	0	25	theme	oxide/chitosan/nano-bentonite	114:142	arg1	oxide/chitosan/nano-bentonite					114:142	nanotitanium oxide/chitosan/nano-bentonite	101:142	nanotitanium oxide/chitosan/nano-bentonite	101:142	Enhanced adsorption of Levofloxacin and Ceftriaxone antibiotics from water by assembled composite of nanotitanium oxide/chitosan/nano-bentonite.					
31924036	5	26	theme	degradation	700:710	arg1	steps					712:716	Four thermal degradation steps	687:716	Four thermal degradation steps	687:716	Four thermal degradation steps were characterized from the TGA of NBent-NTiO2-Chit with a total loss = 23.514% in the temperature range 30-600 °C.					
31924036	9	27	from	enhancement	1340:1350	arg1	percentage					1363:1372	removal percentage	1355:1372	removal percentage of LEVO to 92.4%	1355:1389	The enhancement in removal percentage of LEVO to 92.4% was mainly established by increasing the dose of NBent-NTiO2-Chit to 60.0 mg.					
31924036	4	28	theme	functional	668:677	arg1	groups					679:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	The FT-IR of NBent-NTiO2-Chit confirmed the presence of OH, N-H, Si-O-Al and Si-O-Si functional groups.					
31924036	1	29	theme	crosslinking	214:225	arg1	combination					227:237	the crosslinking combination	210:237	the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent)	210:327	A novel NBent-NTiO2-Chit nanocomposite has been synthesized from the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent) via formaldehyde.					
31924036	8	30	theme	%	1253:1253	arg1	values					1239:1244	Maximum percentage values	1220:1244	Maximum percentage values	1220:1244	Maximum percentage values of 90.2% (pH 4) and 93.5% (pH 5) for LEVO and CFT, respectively, were achieved at 10 min.					
31924036	14	31	theme	CFT	2221:2223	arg1	extraction					2198:2207	extraction	2198:2207	extraction of LEVO and CFT from industrial wastewater	2198:2250	The results proved that the designed NBent-NTiO2-Chit was successively implemented for extraction of LEVO and CFT from industrial wastewater providing percentage values 83.2 and 79.0% using 10.0 and 150.0 ± 1.0 mg NBent-NTiO2-Chit, respectively.					
31924036	10	32	theme	isotherm	1567:1574	arg1	Freundlich					1596:1605	Freundlich	1596:1605	Freundlich	1596:1605	The extraction mechanisms of LEVO and CFT were positively explained by three models of adsorption isotherm including Langmuir, Freundlich and Temkin.					
31924036	10	32	theme	isotherm	1567:1574	arg1	models					1546:1551	three models	1540:1551	three models of adsorption isotherm including Langmuir	1540:1593	The extraction mechanisms of LEVO and CFT were positively explained by three models of adsorption isotherm including Langmuir, Freundlich and Temkin.					
31924036	10	32	theme	isotherm	1567:1574	arg1	Temkin					1611:1616	Temkin	1611:1616	Temkin	1611:1616	The extraction mechanisms of LEVO and CFT were positively explained by three models of adsorption isotherm including Langmuir, Freundlich and Temkin.					
31924036	5	33	theme	NBent-NTiO2-Chit	753:768	arg1	TGA					746:748	the TGA	742:748	the TGA of NBent-NTiO2-Chit with a total loss = 23.514% in the temperature range 30-600 °C	742:831	Four thermal degradation steps were characterized from the TGA of NBent-NTiO2-Chit with a total loss = 23.514% in the temperature range 30-600 °C.					
31924036	3	34	theme	surface	466:472	arg1	16.385 m2 g-1					480:492	16.385 m2 g-1	480:492	16.385 m2 g-1	480:492	The particle size (34-97 nm) and surface area (16.385 m2 g-1) were confirmed from the scanning electron microscopy and BET measurement, respectively.					
31924036	3	34	theme	surface	466:472	arg1	area					474:477	surface area	466:477	surface area (16.385 m2 g-1)	466:493	The particle size (34-97 nm) and surface area (16.385 m2 g-1) were confirmed from the scanning electron microscopy and BET measurement, respectively.					
31924036	4	35	theme	OH	639:640	arg1	groups					679:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	The FT-IR of NBent-NTiO2-Chit confirmed the presence of OH, N-H, Si-O-Al and Si-O-Si functional groups.					
31924036	6	36	theme	antibiotics	911:921	arg1	classes					900:906	two important classes	886:906	two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT)	886:969	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	7	37	theme	LEVO	1171:1174	arg1	ions					1214:1217	interfering ions	1202:1217	interfering ions	1202:1217	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	7	37	theme	LEVO	1171:1174	arg1	dosage					1138:1143	dosage	1138:1143	dosage	1138:1143	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	7	37	theme	LEVO	1171:1174	arg1	concentration					1154:1166	initial concentration	1146:1166	initial concentration of LEVO and CFT	1146:1182	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	7	37	theme	LEVO	1171:1174	arg1	pH					1134:1135	pH	1134:1135	pH	1134:1135	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	7	37	theme	LEVO	1171:1174	arg1	time					1193:1196	contact time	1185:1196	contact time	1185:1196	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	11	38	theme	correlation	1632:1642	arg1	R2					1658:1659	R2	1658:1659	R2	1658:1659	The obtained correlation coefficients (R2) by Langmuir model were 0.952 and 0.987 for LEVO and CFT, respectively to offer excellent fit to the adsorption processes.					
31924036	11	38	theme	correlation	1632:1642	arg1	coefficients					1644:1655	The obtained correlation coefficients	1619:1655	The obtained correlation coefficients (R2) by Langmuir model	1619:1678	The obtained correlation coefficients (R2) by Langmuir model were 0.952 and 0.987 for LEVO and CFT, respectively to offer excellent fit to the adsorption processes.					
31924036	11	38	theme	correlation	1632:1642	arg1	0.952					1685:1689	0.952	1685:1689	0.952	1685:1689	The obtained correlation coefficients (R2) by Langmuir model were 0.952 and 0.987 for LEVO and CFT, respectively to offer excellent fit to the adsorption processes.					
31924036	9	39	theme	NBent-NTiO2-Chit	1440:1455	arg1	dose					1432:1435	the dose	1428:1435	the dose of NBent-NTiO2-Chit to 60.0 mg	1428:1466	The enhancement in removal percentage of LEVO to 92.4% was mainly established by increasing the dose of NBent-NTiO2-Chit to 60.0 mg.					
31924036	0	40	theme	Levofloxacin	23:34	arg1	adsorption					9:18	adsorption	9:18	adsorption of Levofloxacin and Ceftriaxone antibiotics from water	9:73	Enhanced adsorption of Levofloxacin and Ceftriaxone antibiotics from water by assembled composite of nanotitanium oxide/chitosan/nano-bentonite.					
31924036	5	41	theme	total	777:781	arg1	%					796:796	a total loss = 23.514%	775:796	a total loss = 23.514% in the temperature range 30-600 °C	775:831	Four thermal degradation steps were characterized from the TGA of NBent-NTiO2-Chit with a total loss = 23.514% in the temperature range 30-600 °C.					
31924036	2	42	theme	NBent-NTiO2-Chit	371:386	arg1	characterization					351:366	The characterization	347:366	The characterization of NBent-NTiO2-Chit	347:386	The characterization of NBent-NTiO2-Chit was confirmed by different instrumentations.					
31924036	12	43	theme	NBent-NTiO2-Chit	1817:1832	arg1	parameters					1803:1812	The thermodynamics parameters	1784:1812	The thermodynamics parameters of NBent-NTiO2-Chit	1784:1832	The thermodynamics parameters of NBent-NTiO2-Chit were evaluated and referred that the reaction is spontaneous and endothermic.					
31924036	1	44	theme	nanotitanium	242:253	arg1	oxide-chitosan					255:268	nanotitanium oxide-chitosan	242:268	nanotitanium oxide-chitosan (NTiO2-Chit)	242:281	A novel NBent-NTiO2-Chit nanocomposite has been synthesized from the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent) via formaldehyde.					
31924036	1	44	theme	nanotitanium	242:253	arg1	NTiO2-Chit					271:280	NTiO2-Chit	271:280	NTiO2-Chit	271:280	A novel NBent-NTiO2-Chit nanocomposite has been synthesized from the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent) via formaldehyde.					
31924036	4	45	theme	N-H	643:645	arg1	groups					679:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	OH, N-H, Si-O-Al and Si-O-Si functional groups	639:684	The FT-IR of NBent-NTiO2-Chit confirmed the presence of OH, N-H, Si-O-Al and Si-O-Si functional groups.					
31924036	7	46	theme	initial	1146:1152	arg1	concentration					1154:1166	initial concentration	1146:1166	initial concentration of LEVO and CFT	1146:1182	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	8	47	theme	percentage	1228:1237	arg1	values					1239:1244	Maximum percentage values	1220:1244	Maximum percentage values	1220:1244	Maximum percentage values of 90.2% (pH 4) and 93.5% (pH 5) for LEVO and CFT, respectively, were achieved at 10 min.					
31924036	1	48	theme	oxide-chitosan	255:268	arg1	combination					227:237	the crosslinking combination	210:237	the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent)	210:327	A novel NBent-NTiO2-Chit nanocomposite has been synthesized from the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent) via formaldehyde.					
31924036	0	49	theme	Ceftriaxone	40:50	arg1	adsorption					9:18	adsorption	9:18	adsorption of Levofloxacin and Ceftriaxone antibiotics from water	9:73	Enhanced adsorption of Levofloxacin and Ceftriaxone antibiotics from water by assembled composite of nanotitanium oxide/chitosan/nano-bentonite.					
31924036	3	50	theme	scanning	519:526	arg1	microscopy					537:546	the scanning electron microscopy	515:546	the scanning electron microscopy	515:546	The particle size (34-97 nm) and surface area (16.385 m2 g-1) were confirmed from the scanning electron microscopy and BET measurement, respectively.					
31924036	14	51	theme	designed	2139:2146	arg1	NBent-NTiO2-Chit					2148:2163	the designed NBent-NTiO2-Chit	2135:2163	the designed NBent-NTiO2-Chit	2135:2163	The results proved that the designed NBent-NTiO2-Chit was successively implemented for extraction of LEVO and CFT from industrial wastewater providing percentage values 83.2 and 79.0% using 10.0 and 150.0 ± 1.0 mg NBent-NTiO2-Chit, respectively.					
31924036	9	52	theme	removal	1355:1361	arg1	percentage					1363:1372	removal percentage	1355:1372	removal percentage of LEVO to 92.4%	1355:1389	The enhancement in removal percentage of LEVO to 92.4% was mainly established by increasing the dose of NBent-NTiO2-Chit to 60.0 mg.					
31924036	10	53	theme	extraction	1473:1482	arg1	mechanisms					1484:1493	The extraction mechanisms	1469:1493	The extraction mechanisms of LEVO and CFT	1469:1509	The extraction mechanisms of LEVO and CFT were positively explained by three models of adsorption isotherm including Langmuir, Freundlich and Temkin.					
31924036	10	54	theme	LEVO	1498:1501	arg1	mechanisms					1484:1493	The extraction mechanisms	1469:1493	The extraction mechanisms of LEVO and CFT	1469:1509	The extraction mechanisms of LEVO and CFT were positively explained by three models of adsorption isotherm including Langmuir, Freundlich and Temkin.					
31924036	9	55	theme	%	1389:1389	arg1	percentage					1363:1372	removal percentage	1355:1372	removal percentage of LEVO to 92.4%	1355:1389	The enhancement in removal percentage of LEVO to 92.4% was mainly established by increasing the dose of NBent-NTiO2-Chit to 60.0 mg.					
31924036	5	56	from	%	796:796	arg1	range					817:821	the temperature range 30-600 °C	801:831	the temperature range 30-600 °C	801:831	Four thermal degradation steps were characterized from the TGA of NBent-NTiO2-Chit with a total loss = 23.514% in the temperature range 30-600 °C.					
31924036	3	57	theme	particle	437:444	arg1	34-97 nm					452:459	34-97 nm	452:459	34-97 nm	452:459	The particle size (34-97 nm) and surface area (16.385 m2 g-1) were confirmed from the scanning electron microscopy and BET measurement, respectively.					
31924036	3	57	theme	particle	437:444	arg1	size					446:449	The particle size	433:449	The particle size (34-97 nm)	433:460	The particle size (34-97 nm) and surface area (16.385 m2 g-1) were confirmed from the scanning electron microscopy and BET measurement, respectively.					
31924036	4	58	theme	NBent-NTiO2-Chit	596:611	arg1	FT-IR					587:591	The FT-IR	583:591	The FT-IR of NBent-NTiO2-Chit	583:611	The FT-IR of NBent-NTiO2-Chit confirmed the presence of OH, N-H, Si-O-Al and Si-O-Si functional groups.					
31924036	0	59	from	water	69:73	arg1	adsorption					9:18	adsorption	9:18	adsorption of Levofloxacin and Ceftriaxone antibiotics from water	9:73	Enhanced adsorption of Levofloxacin and Ceftriaxone antibiotics from water by assembled composite of nanotitanium oxide/chitosan/nano-bentonite.					
31924036	9	60	dep	92.4	1385:1388	arg1	to					1382:1383	to	1382:1383	to	1382:1383	The enhancement in removal percentage of LEVO to 92.4% was mainly established by increasing the dose of NBent-NTiO2-Chit to 60.0 mg.					
31924036	6	61	theme	assembled	838:846	arg1	nanocomposite					848:860	The assembled nanocomposite	834:860	The assembled nanocomposite	834:860	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	7	62	theme	interfering	1202:1212	arg1	ions					1214:1217	interfering ions	1202:1217	interfering ions	1202:1217	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	3	63	theme	BET	552:554	arg1	measurement					556:566	BET measurement	552:566	BET measurement	552:566	The particle size (34-97 nm) and surface area (16.385 m2 g-1) were confirmed from the scanning electron microscopy and BET measurement, respectively.					
31924036	7	64	theme	extraction	1088:1097	arg1	percentage					1074:1083	the percentage	1070:1083	the percentage of extraction	1070:1097	The various factors that affected the percentage of extraction were applied and optimized such as pH, dosage, initial concentration of LEVO and CFT, contact time and interfering ions.					
31924036	1	65	theme	novel	147:151	arg1	nanocomposite					170:182	A novel NBent-NTiO2-Chit nanocomposite	145:182	A novel NBent-NTiO2-Chit nanocomposite	145:182	A novel NBent-NTiO2-Chit nanocomposite has been synthesized from the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent) via formaldehyde.					
31924036	6	66	theme	Cephalosporin	1007:1019	arg1	forms					978:982	the forms	974:982	the forms of Fluoroquinolone and Cephalosporin	974:1019	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	10	67	theme	CFT	1507:1509	arg1	mechanisms					1484:1493	The extraction mechanisms	1469:1493	The extraction mechanisms of LEVO and CFT	1469:1509	The extraction mechanisms of LEVO and CFT were positively explained by three models of adsorption isotherm including Langmuir, Freundlich and Temkin.					
31924036	6	68	theme	Levofloxacin	933:944	arg1	CFT					966:968	CFT	966:968	CFT	966:968	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	6	68	theme	Levofloxacin	933:944	arg1	Ceftriaxone					953:963	Levofloxacin (LEVO) Ceftriaxone	933:963	Levofloxacin (LEVO) Ceftriaxone (CFT)	933:969	The assembled nanocomposite enhanced the removal of two important classes of antibiotics including Levofloxacin (LEVO) Ceftriaxone (CFT) in the forms of Fluoroquinolone and Cephalosporin, respectively.					
31924036	0	69	dep	Levofloxacin	23:34	arg1	antibiotics					52:62	antibiotics	52:62	antibiotics	52:62	Enhanced adsorption of Levofloxacin and Ceftriaxone antibiotics from water by assembled composite of nanotitanium oxide/chitosan/nano-bentonite.					
31924036	13	70	theme	acquired	2041:2048	arg1	values					2053:2058	the acquired R2 values	2037:2058	the acquired R2 values	2037:2058	The kinetic studies of NBent-NTiO2-Chit with LEVO and CFT antibiotics were better fitted by the pseudo-second-order based on the acquired R2 values as 0.999 and 0.997 for LEVO and CFT, respectively.					
31924036	1	71	theme	NBent-NTiO2-Chit	153:168	arg1	nanocomposite					170:182	A novel NBent-NTiO2-Chit nanocomposite	145:182	A novel NBent-NTiO2-Chit nanocomposite	145:182	A novel NBent-NTiO2-Chit nanocomposite has been synthesized from the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent) via formaldehyde.					
31924036	13	72	theme	kinetic	1916:1922	arg1	studies					1924:1930	The kinetic studies	1912:1930	The kinetic studies of NBent-NTiO2-Chit with LEVO and CFT antibiotics	1912:1980	The kinetic studies of NBent-NTiO2-Chit with LEVO and CFT antibiotics were better fitted by the pseudo-second-order based on the acquired R2 values as 0.999 and 0.997 for LEVO and CFT, respectively.					
31924036	4	73	theme	groups	679:684	arg1	presence					627:634	the presence	623:634	the presence of OH, N-H, Si-O-Al and Si-O-Si functional groups	623:684	The FT-IR of NBent-NTiO2-Chit confirmed the presence of OH, N-H, Si-O-Al and Si-O-Si functional groups.					
31924036	2	74	theme	different	405:413	arg1	instrumentations					415:430	different instrumentations	405:430	different instrumentations	405:430	The characterization of NBent-NTiO2-Chit was confirmed by different instrumentations.					
31924036	3	75	theme	electron	528:535	arg1	microscopy					537:546	the scanning electron microscopy	515:546	the scanning electron microscopy	515:546	The particle size (34-97 nm) and surface area (16.385 m2 g-1) were confirmed from the scanning electron microscopy and BET measurement, respectively.					
31924036	11	76	theme	obtained	1623:1630	arg1	R2					1658:1659	R2	1658:1659	R2	1658:1659	The obtained correlation coefficients (R2) by Langmuir model were 0.952 and 0.987 for LEVO and CFT, respectively to offer excellent fit to the adsorption processes.					
31924036	11	76	theme	obtained	1623:1630	arg1	coefficients					1644:1655	The obtained correlation coefficients	1619:1655	The obtained correlation coefficients (R2) by Langmuir model	1619:1678	The obtained correlation coefficients (R2) by Langmuir model were 0.952 and 0.987 for LEVO and CFT, respectively to offer excellent fit to the adsorption processes.					
31924036	11	76	theme	obtained	1623:1630	arg1	0.952					1685:1689	0.952	1685:1689	0.952	1685:1689	The obtained correlation coefficients (R2) by Langmuir model were 0.952 and 0.987 for LEVO and CFT, respectively to offer excellent fit to the adsorption processes.					
31924036	8	77	theme	%	1270:1270	arg1	values					1239:1244	Maximum percentage values	1220:1244	Maximum percentage values	1220:1244	Maximum percentage values of 90.2% (pH 4) and 93.5% (pH 5) for LEVO and CFT, respectively, were achieved at 10 min.					
31924036	1	78	theme	nanotitanium	287:298	arg1	NTiO2-Bent					317:326	NTiO2-Bent	317:326	NTiO2-Bent	317:326	A novel NBent-NTiO2-Chit nanocomposite has been synthesized from the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent) via formaldehyde.					
31924036	1	78	theme	nanotitanium	287:298	arg1	oxide-bentonite					300:314	nanotitanium oxide-bentonite	287:314	nanotitanium oxide-bentonite (NTiO2-Bent)	287:327	A novel NBent-NTiO2-Chit nanocomposite has been synthesized from the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent) via formaldehyde.					
31924036	11	79	theme	excellent	1741:1749	arg1	fit					1751:1753	excellent fit	1741:1753	excellent fit to the adsorption processes	1741:1781	The obtained correlation coefficients (R2) by Langmuir model were 0.952 and 0.987 for LEVO and CFT, respectively to offer excellent fit to the adsorption processes.					
31924036	13	80	theme	NBent-NTiO2-Chit	1935:1950	arg1	studies					1924:1930	The kinetic studies	1912:1930	The kinetic studies of NBent-NTiO2-Chit with LEVO and CFT antibiotics	1912:1980	The kinetic studies of NBent-NTiO2-Chit with LEVO and CFT antibiotics were better fitted by the pseudo-second-order based on the acquired R2 values as 0.999 and 0.997 for LEVO and CFT, respectively.					
31924036	5	81	with	TGA	746:748	arg1	%					796:796	a total loss = 23.514%	775:796	a total loss = 23.514% in the temperature range 30-600 °C	775:831	Four thermal degradation steps were characterized from the TGA of NBent-NTiO2-Chit with a total loss = 23.514% in the temperature range 30-600 °C.					
31924036	13	82	dep	LEVO	1957:1960	arg1	antibiotics					1970:1980	antibiotics	1970:1980	antibiotics	1970:1980	The kinetic studies of NBent-NTiO2-Chit with LEVO and CFT antibiotics were better fitted by the pseudo-second-order based on the acquired R2 values as 0.999 and 0.997 for LEVO and CFT, respectively.					
31924036	1	83	theme	oxide-bentonite	300:314	arg1	combination					227:237	the crosslinking combination	210:237	the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent)	210:327	A novel NBent-NTiO2-Chit nanocomposite has been synthesized from the crosslinking combination of nanotitanium oxide-chitosan (NTiO2-Chit) and nanotitanium oxide-bentonite (NTiO2-Bent) via formaldehyde.					
31353557	9	0	from	starch	1672:1677	arg1	rich					1653:1656	rich	1653:1656	rich	1653:1656	The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.					
31353557	3	1	contain	possessed	649:657	arg2	stability					699:707	superior thermal stability	682:707	superior thermal stability	682:707	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	3	1	contain	possessed	649:657	arg1	glucoamylase					628:639	glucoamylase	628:639	glucoamylase	628:639	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	3	1	contain	possessed	649:657	arg2	tolerance					668:676	wider pH tolerance	659:676	wider pH tolerance	659:676	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	3	1	contain	possessed	649:657	arg1	pectinase					614:622	pectinase	614:622	pectinase	614:622	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	2	2	theme	activity	557:564	arg1	recovery					566:573	activity recovery	557:573	activity recovery of 73.8% and 85.2%, respectively	557:606	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	6	3	from	content	1071:1077	arg1	juice					1096:1100	the resulting juice	1082:1100	the resulting juice	1082:1100	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	1	4	theme	pectinase	188:196	arg1	Co-immobilization					167:183	Co-immobilization	167:183	Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads	167:255	Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as activating agent.					
31353557	6	5	theme	reducing	1056:1063	arg1	content					1071:1077	reducing sugar content	1056:1077	reducing sugar content in the resulting juice	1056:1100	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	2	6	theme	%	592:592	arg1	recovery					566:573	activity recovery	557:573	activity recovery of 73.8% and 85.2%, respectively	557:606	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	8	7	theme	sodium	1488:1493	arg1	beads					1519:1523	sodium alginate/graphene oxide beads	1488:1523	sodium alginate/graphene oxide beads	1488:1523	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.					
31353557	2	8	theme	co-immobilized	349:362	arg1	I-PG					388:391	I-PG	388:391	I-PG	388:391	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	2	8	theme	co-immobilized	349:362	arg1	pectinase-glucoamylase					364:385	The co-immobilized pectinase-glucoamylase	345:385	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min)	345:452	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	9	9	theme	juice	1647:1651	arg1	clarification					1630:1642	the clarification	1626:1642	the clarification of juice rich in pectin and starch	1626:1677	The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.					
31353557	1	10	theme	activating	327:336	arg1	agent					338:342	activating agent	327:342	activating agent	327:342	Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as activating agent.					
31353557	7	11	theme	effective	1232:1240	arg1	method					1255:1260	an effective and feasible method	1229:1260	an effective and feasible method for improving quality of the pumpkin-hawthorn juice	1229:1312	Therefore, the use of I-PG provided an effective and feasible method for improving quality of the pumpkin-hawthorn juice.					
31353557	9	12	theme	rich	1653:1656	arg1	clarification					1630:1642	the clarification	1626:1642	the clarification of juice rich in pectin and starch	1626:1677	The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.					
31353557	4	13	from	enzymes	786:792	arg1	I-PG					797:800	I-PG	797:800	I-PG	797:800	Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse.					
31353557	5	14	theme	compound	920:927	arg1	juice					929:933	the pumpkin-hawthorn compound juice	899:933	the pumpkin-hawthorn compound juice	899:933	Conditions for the hydrolysis of the pumpkin-hawthorn compound juice by I-PG were optimized using orthogonal experiments.					
31353557	2	15	theme	%	582:582	arg1	recovery					566:573	activity recovery	557:573	activity recovery of 73.8% and 85.2%, respectively	557:606	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	6	16	theme	sugar	1065:1069	arg1	content					1071:1077	reducing sugar content	1056:1077	reducing sugar content in the resulting juice	1056:1100	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	6	17	dep	increased	1102:1110	arg1	whereas					1127:1133	whereas	1127:1133	whereas	1127:1133	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	4	18	theme	activity	831:838	arg1	%					818:818	60%	816:818	60% of initial activity	816:838	Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse.					
31353557	4	18	theme	activity	831:838	arg1	activity					831:838	initial activity	823:838	initial activity	823:838	Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse.					
31353557	6	19	theme	pectin	1155:1160	arg1	content					1162:1168	soluble protein and pectin content	1135:1168	content	1162:1168	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	1	20	theme	glucoamylase	202:213	arg1	Co-immobilization					167:183	Co-immobilization	167:183	Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads	167:255	Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as activating agent.					
31353557	9	21	from	pectin	1661:1666	arg1	rich					1653:1656	rich	1653:1656	rich	1653:1656	The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.					
31353557	6	22	from	solids	1044:1049	arg1	juice					1096:1100	the resulting juice	1082:1100	the resulting juice	1082:1100	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	7	23	theme	pumpkin-hawthorn	1291:1306	arg1	juice					1308:1312	the pumpkin-hawthorn juice	1287:1312	the pumpkin-hawthorn juice	1287:1312	Therefore, the use of I-PG provided an effective and feasible method for improving quality of the pumpkin-hawthorn juice.					
31353557	6	24	theme	soluble	1135:1141	arg1	protein					1143:1149	soluble protein and pectin content	1135:1168	protein	1143:1149	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	2	25	theme	glucoamylase	508:519	arg1	activity					521:528	glucoamylase activity	508:528	glucoamylase activity of 1,027.2 ± 29.2U/g	508:549	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	8	26	theme	PRACTICAL	1315:1323	arg1	APPLICATIONS					1325:1336	PRACTICAL APPLICATIONS	1315:1336	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.	1315:1538	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.					
31353557	8	27	dep	APPLICATIONS	1325:1336	arg1	developed					1529:1537	developed	1529:1537	was developed	1525:1537	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.					
31353557	4	28	theme	initial	823:829	arg1	activity					831:838	initial activity	823:838	initial activity	823:838	Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse.					
31353557	1	29	theme	sodium	220:225	arg1	beads					251:255	sodium alginate/graphene oxide beads	220:255	sodium alginate/graphene oxide beads	220:255	Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as activating agent.					
31353557	6	30	theme	soluble	1036:1042	arg1	solids					1044:1049	soluble solids	1036:1049	soluble solids	1036:1049	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	2	31	dep	conditions	417:426	arg1	35 min					446:451	35 min	446:451	35 min	446:451	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	2	31	dep	conditions	417:426	arg1	pH					429:430	pH 4.0	429:434	pH 4.0	429:434	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	2	31	dep	conditions	417:426	arg1	40°C					437:440	40°C	437:440	40°C	437:440	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	1	32	theme	alginate/graphene	227:243	arg1	beads					251:255	sodium alginate/graphene oxide beads	220:255	sodium alginate/graphene oxide beads	220:255	Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as activating agent.					
31353557	2	33	theme	1,227.5 ± 36.5U/g	486:502	arg1	activity					521:528	glucoamylase activity	508:528	glucoamylase activity of 1,027.2 ± 29.2U/g	508:549	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	2	33	theme	1,227.5 ± 36.5U/g	486:502	arg1	activity					474:481	pectinase activity	464:481	pectinase activity of 1,227.5 ± 36.5U/g	464:502	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	6	34	from	transmittance	1021:1033	arg1	juice					1096:1100	the resulting juice	1082:1100	the resulting juice	1082:1100	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	5	35	theme	orthogonal	964:973	arg1	experiments					975:985	orthogonal experiments	964:985	orthogonal experiments	964:985	Conditions for the hydrolysis of the pumpkin-hawthorn compound juice by I-PG were optimized using orthogonal experiments.					
31353557	1	36	theme	oxide	245:249	arg1	beads					251:255	sodium alginate/graphene oxide beads	220:255	sodium alginate/graphene oxide beads	220:255	Co-immobilization of pectinase and glucoamylase onto sodium alginate/graphene oxide beads was achieved by N,N'-dicyclohexylcarbodiimide/N-hydroxysuccinimide as activating agent.					
31353557	7	37	theme	I-PG	1215:1218	arg1	use					1208:1210	the use	1204:1210	the use of I-PG	1204:1218	Therefore, the use of I-PG provided an effective and feasible method for improving quality of the pumpkin-hawthorn juice.					
31353557	0	38	theme	juice	160:164	arg1	preparation					128:138	the preparation	124:138	the preparation of pumpkin-hawthorn juice	124:164	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	0	39	from	beads	95:99	arg1	preparation					128:138	the preparation	124:138	the preparation of pumpkin-hawthorn juice	124:164	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	3	40	theme	wider	659:663	arg1	tolerance					668:676	wider pH tolerance	659:676	wider pH tolerance	659:676	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	9	41	from	rich	1653:1656	arg1	starch					1672:1677	starch	1672:1677	starch	1672:1677	The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.					
31353557	9	41	from	rich	1653:1656	arg1	pectin					1661:1666	pectin	1661:1666	pectin	1661:1666	The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.					
31353557	2	42	theme	pectinase	464:472	arg1	activity					474:481	pectinase activity	464:481	pectinase activity of 1,227.5 ± 36.5U/g	464:502	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	0	43	theme	glucoamylase	35:46	arg1	Co-immobilization					0:16	Co-immobilization	0:16	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.	0:165	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	6	44	theme	light	1015:1019	arg1	transmittance					1021:1033	light transmittance	1015:1033	light transmittance	1015:1033	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	0	45	theme	pumpkin-hawthorn	143:158	arg1	juice					160:164	pumpkin-hawthorn juice	143:164	pumpkin-hawthorn juice	143:164	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	3	46	theme	pH	665:666	arg1	tolerance					668:676	wider pH tolerance	659:676	wider pH tolerance	659:676	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	5	47	theme	pumpkin-hawthorn	903:918	arg1	juice					929:933	the pumpkin-hawthorn compound juice	899:933	the pumpkin-hawthorn compound juice	899:933	Conditions for the hydrolysis of the pumpkin-hawthorn compound juice by I-PG were optimized using orthogonal experiments.					
31353557	2	48	theme	1,027.2 ± 29.2U/g	533:549	arg1	activity					521:528	glucoamylase activity	508:528	glucoamylase activity of 1,027.2 ± 29.2U/g	508:549	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	2	48	theme	1,027.2 ± 29.2U/g	533:549	arg1	activity					474:481	pectinase activity	464:481	pectinase activity of 1,227.5 ± 36.5U/g	464:502	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	7	49	theme	juice	1308:1312	arg1	quality					1276:1282	quality	1276:1282	quality of the pumpkin-hawthorn juice	1276:1312	Therefore, the use of I-PG provided an effective and feasible method for improving quality of the pumpkin-hawthorn juice.					
31353557	0	50	theme	oxide	79:83	arg1	beads					95:99	sodium aliginate/graphene oxide composite beads	53:99	sodium aliginate/graphene oxide composite beads	53:99	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	0	51	theme	sodium	53:58	arg1	beads					95:99	sodium aliginate/graphene oxide composite beads	53:99	sodium aliginate/graphene oxide composite beads	53:99	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	2	52	contain	possessed	454:462	arg1	I-PG					388:391	I-PG	388:391	I-PG	388:391	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	2	52	contain	possessed	454:462	arg2	activity					474:481	pectinase activity	464:481	pectinase activity of 1,227.5 ± 36.5U/g	464:502	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	2	52	contain	possessed	454:462	arg2	activity					521:528	glucoamylase activity	508:528	glucoamylase activity of 1,027.2 ± 29.2U/g	508:549	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	2	52	contain	possessed	454:462	arg1	pectinase-glucoamylase					364:385	The co-immobilized pectinase-glucoamylase	345:385	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min)	345:452	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	8	53	theme	enzymes	1475:1481	arg1	co-immobilization					1444:1460	the co-immobilization	1440:1460	the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads	1440:1523	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.					
31353557	2	54	theme	optimal	409:415	arg1	conditions					417:426	optimal conditions	409:426	optimal conditions (pH 4.0, 40°C and 35 min)	409:452	The co-immobilized pectinase-glucoamylase (I-PG) prepared under optimal conditions (pH 4.0, 40°C and 35 min) possessed pectinase activity of 1,227.5 ± 36.5U/g and glucoamylase activity of 1,027.2 ± 29.2U/g, with activity recovery of 73.8% and 85.2%, respectively.					
31353557	3	55	from	pectinase	614:622	arg1	I-PG					644:647	I-PG	644:647	I-PG	644:647	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	8	56	theme	oxide	1513:1517	arg1	beads					1519:1523	sodium alginate/graphene oxide beads	1488:1523	sodium alginate/graphene oxide beads	1488:1523	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.					
31353557	8	57	theme	alginate/graphene	1495:1511	arg1	beads					1519:1523	sodium alginate/graphene oxide beads	1488:1523	sodium alginate/graphene oxide beads	1488:1523	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.					
31353557	3	58	theme	superior	682:689	arg1	stability					699:707	superior thermal stability	682:707	superior thermal stability	682:707	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	6	59	with	treatment	994:1002	arg1	I-PG					1009:1012	I-PG	1009:1012	I-PG	1009:1012	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	6	60	theme	resulting	1086:1094	arg1	juice					1096:1100	the resulting juice	1082:1100	the resulting juice	1082:1100	After treatment with I-PG, light transmittance, soluble solids, and reducing sugar content in the resulting juice increased significantly, whereas soluble protein and pectin content decreased appreciably.					
31353557	8	61	theme	effective	1419:1427	arg1	method					1429:1434	an effective method	1416:1434	an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads	1416:1523	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.					
31353557	0	62	from	application	109:119	arg1	preparation					128:138	the preparation	124:138	the preparation of pumpkin-hawthorn juice	124:164	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	3	63	theme	thermal	691:697	arg1	stability					699:707	superior thermal stability	682:707	superior thermal stability	682:707	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	8	64	dep	overcome	1351:1358	arg1	to					1348:1349	to	1348:1349	to	1348:1349	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.					
31353557	8	65	theme	free	1383:1386	arg1	pectinase					1388:1396	free pectinase	1383:1396	free pectinase	1383:1396	PRACTICAL APPLICATIONS: In order to overcome the drawbacks of using free pectinase and glucoamylase, an effective method for the co-immobilization of these two enzymes onto sodium alginate/graphene oxide beads was developed.					
31353557	3	66	from	glucoamylase	628:639	arg1	I-PG					644:647	I-PG	644:647	I-PG	644:647	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	0	67	theme	composite	85:93	arg1	beads					95:99	sodium aliginate/graphene oxide composite beads	53:99	sodium aliginate/graphene oxide composite beads	53:99	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	3	68	theme	free	727:730	arg1	counterparts					732:743	their free counterparts	721:743	their free counterparts	721:743	Both pectinase and glucoamylase in I-PG possessed wider pH tolerance and superior thermal stability to those of their free counterparts.					
31353557	4	69	theme	reuse	859:863	arg1	times					850:854	six times	846:854	six times of reuse	846:863	Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse.					
31353557	9	70	theme	co-immobilized	1544:1557	arg1	pectinase/glucoamylase					1559:1580	The co-immobilized pectinase/glucoamylase	1540:1580	The co-immobilized pectinase/glucoamylase developed in this study	1540:1604	The co-immobilized pectinase/glucoamylase developed in this study could be applied in the clarification of juice rich in pectin and starch.					
31353557	5	71	theme	juice	929:933	arg1	hydrolysis					885:894	the hydrolysis	881:894	the hydrolysis of the pumpkin-hawthorn compound juice by I-PG	881:941	Conditions for the hydrolysis of the pumpkin-hawthorn compound juice by I-PG were optimized using orthogonal experiments.					
31353557	0	72	theme	aliginate/graphene	60:77	arg1	beads					95:99	sodium aliginate/graphene oxide composite beads	53:99	sodium aliginate/graphene oxide composite beads	53:99	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	0	73	theme	pectinase	21:29	arg1	Co-immobilization					0:16	Co-immobilization	0:16	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.	0:165	Co-immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin-hawthorn juice.					
31353557	4	74	theme	Reusability	746:756	arg1	studies					758:764	Reusability studies	746:764	Reusability studies	746:764	Reusability studies indicated that both enzymes in I-PG retained over 60% of initial activity after six times of reuse.					
31353557	7	75	theme	feasible	1246:1253	arg1	method					1255:1260	an effective and feasible method	1229:1260	an effective and feasible method for improving quality of the pumpkin-hawthorn juice	1229:1312	Therefore, the use of I-PG provided an effective and feasible method for improving quality of the pumpkin-hawthorn juice.					
31392330	6	0	theme	cellulose	984:992	arg1	grass					1051:1055	coarse Miscanthus grass	1033:1055	coarse Miscanthus grass (MG)	1033:1060	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	%					955:955	3%	954:955	3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG	954:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	BP					1029:1030	fine BP	1024:1030	fine BP	1024:1030	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	cellulose					984:992	cellulose	984:992	cellulose (CEL)	984:998	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	MG					1058:1059	MG	1058:1059	MG	1058:1059	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	pulp					1013:1016	coarse beet pulp	1001:1016	coarse beet pulp (BP)	1001:1021	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	CEL					995:997	CEL	995:997	CEL	995:997	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	sepiolite					967:975	either sepiolite	960:975	either sepiolite (SEP)	960:981	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	BP					1019:1020	BP	1019:1020	BP	1019:1020	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	SEP					978:980	SEP	978:980	SEP	978:980	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	0	theme	cellulose	984:992	arg1	MG					1072:1073	fine MG	1067:1073	fine MG	1067:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	11	1	theme	digestibility	1752:1764	arg1	coefficients					1766:1777	digestibility coefficients	1752:1777	digestibility coefficients of birds fed BP diets	1752:1799	Birds fed diets containing MG performed similar to chicks fed CEL (P > 0.05), but digestibility coefficients of birds fed BP diets were generally higher than chicks fed MG diets.					
31392330	10	2	theme	fiber	1610:1614	arg1	particles					1616:1624	coarse fiber particles	1603:1624	coarse fiber particles	1603:1624	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	10	3	theme	coarse	1603:1608	arg1	particles					1616:1624	coarse fiber particles	1603:1624	coarse fiber particles	1603:1624	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	10	4	theme	fed	1644:1646	arg1	particles					1659:1667	these fed fine fiber particles	1638:1667	these fed fine fiber particles	1638:1667	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	9	5	theme	better	1416:1421	arg1	rate					1439:1442	better feed conversion rate	1416:1442	better feed conversion rate	1416:1442	In general, chicks fed diets containing fiber consumed more feed, gained more weight, and had better feed conversion rate than birds fed the SEP diet.					
31392330	6	6	theme	fine	1024:1027	arg1	BP					1029:1030	fine BP	1024:1030	fine BP	1024:1030	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	11	7	theme	MG	1839:1840	arg1	diets					1842:1846	MG diets	1839:1846	MG diets	1839:1846	Birds fed diets containing MG performed similar to chicks fed CEL (P > 0.05), but digestibility coefficients of birds fed BP diets were generally higher than chicks fed MG diets.					
31392330	5	8	theme	particle	799:806	arg1	size					808:811	particle size	799:811	particle size	799:811	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	9	9	theme	conversion	1428:1437	arg1	rate					1439:1442	better feed conversion rate	1416:1442	better feed conversion rate	1416:1442	In general, chicks fed diets containing fiber consumed more feed, gained more weight, and had better feed conversion rate than birds fed the SEP diet.					
31392330	6	10	theme	fine	1067:1070	arg1	MG					1072:1073	fine MG	1067:1073	fine MG	1067:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	11	11	contain	containing	1686:1695	arg2	MG					1697:1698	MG	1697:1698	MG	1697:1698	Birds fed diets containing MG performed similar to chicks fed CEL (P > 0.05), but digestibility coefficients of birds fed BP diets were generally higher than chicks fed MG diets.					
31392330	11	11	contain	containing	1686:1695	arg1	diets					1680:1684	diets	1680:1684	diets containing MG	1680:1698	Birds fed diets containing MG performed similar to chicks fed CEL (P > 0.05), but digestibility coefficients of birds fed BP diets were generally higher than chicks fed MG diets.					
31392330	7	12	theme	daily	1172:1176	arg1	gain					1178:1181	average daily gain	1164:1181	average daily gain	1164:1181	At the end of days 7, 14, and 21, chicks and experimental diets were weighed to compute average daily gain and feed intake.					
31392330	9	13	contain	containing	1351:1360	arg2	fiber					1362:1366	fiber	1362:1366	fiber	1362:1366	In general, chicks fed diets containing fiber consumed more feed, gained more weight, and had better feed conversion rate than birds fed the SEP diet.					
31392330	9	13	contain	containing	1351:1360	arg1	diets					1345:1349	diets	1345:1349	diets containing fiber	1345:1366	In general, chicks fed diets containing fiber consumed more feed, gained more weight, and had better feed conversion rate than birds fed the SEP diet.					
31392330	10	14	theme	fiber	1653:1657	arg1	particles					1659:1667	these fed fine fiber particles	1638:1667	these fed fine fiber particles	1638:1667	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	5	15	theme	dietary	774:780	arg1	source					788:793	dietary fiber source	774:793	dietary fiber source	774:793	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	11	16	theme	P	1737:1737	arg1	>					1739:1739	P > 0.05	1737:1744	P > 0.05	1737:1744	Birds fed diets containing MG performed similar to chicks fed CEL (P > 0.05), but digestibility coefficients of birds fed BP diets were generally higher than chicks fed MG diets.					
31392330	6	17	dep	chicks	878:883	arg1	cages					907:911	5 cages	905:911	5 cages per treatment	905:925	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	17	dep	chicks	878:883	arg1	chicks					888:893	8 chicks	886:893	8 chicks per cage	886:902	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	5	18	theme	source	788:793	arg1	effect					764:769	the effect	760:769	the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility	760:867	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	9	19	theme	SEP	1463:1465	arg1	diet					1467:1470	the SEP diet	1459:1470	the SEP diet	1459:1470	In general, chicks fed diets containing fiber consumed more feed, gained more weight, and had better feed conversion rate than birds fed the SEP diet.					
31392330	4	20	theme	consistent	649:658	arg1	composition					666:676	a consistent fiber composition	647:676	a consistent fiber composition	647:676	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	10	21	theme	chick	1517:1521	arg1	performance					1523:1533	chick performance	1517:1533	chick performance	1517:1533	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	1	22	theme	fiber	104:108	arg1	addition					92:99	The addition	88:99	The addition of fiber in chick feeds	88:123	The addition of fiber in chick feeds is known to dilute nutrients; as a result, this may reduce nutrient digestibility and performance.					
31392330	8	23	theme	previous	1230:1237	arg1	48 h					1239:1242	the previous 48 h	1226:1242	the previous 48 h of each data capture point	1226:1269	In addition, excreta from the previous 48 h of each data capture point was collected to determine nutrient digestibility.					
31392330	6	24	theme	Miscanthus	1040:1049	arg1	grass					1051:1055	coarse Miscanthus grass	1033:1055	coarse Miscanthus grass (MG)	1033:1060	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	24	theme	Miscanthus	1040:1049	arg1	MG					1058:1059	MG	1058:1059	MG	1058:1059	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	0	25	theme	chick	44:48	arg1	performance					50:60	chick performance	44:60	chick performance	44:60	Effect of fiber source and particle size on chick performance and nutrient utilization.					
31392330	5	26	theme	chick	824:828	arg1	performance					830:840	day-old chick performance	816:840	day-old chick performance	816:840	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	8	27	theme	data	1252:1255	arg1	point					1265:1269	each data capture point	1247:1269	each data capture point	1247:1269	In addition, excreta from the previous 48 h of each data capture point was collected to determine nutrient digestibility.					
31392330	3	28	from	fluctuation	471:481	arg1	composition					517:527	their nutritional and chemical composition	486:527	their nutritional and chemical composition due to variation in processing	486:558	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31392330	10	29	contain	had	1500:1502	arg1	size					1482:1485	Particle size	1473:1485	Particle size of the fiber	1473:1498	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	10	29	contain	had	1500:1502	arg2	effect					1507:1512	no effect	1504:1512	no effect	1504:1512	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	8	30	theme	point	1265:1269	arg1	48 h					1239:1242	the previous 48 h	1226:1242	the previous 48 h of each data capture point	1226:1269	In addition, excreta from the previous 48 h of each data capture point was collected to determine nutrient digestibility.					
31392330	2	31	dep	3	306:306	arg1	to					303:304	to	303:304	to	303:304	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	9	32	contain	had	1412:1414	arg2	rate					1439:1442	better feed conversion rate	1416:1442	better feed conversion rate	1416:1442	In general, chicks fed diets containing fiber consumed more feed, gained more weight, and had better feed conversion rate than birds fed the SEP diet.					
31392330	9	32	contain	had	1412:1414	arg1	chicks					1334:1339	chicks	1334:1339	chicks fed diets containing fiber	1334:1366	In general, chicks fed diets containing fiber consumed more feed, gained more weight, and had better feed conversion rate than birds fed the SEP diet.					
31392330	6	33	theme	sepiolite	967:975	arg1	grass					1051:1055	coarse Miscanthus grass	1033:1055	coarse Miscanthus grass (MG)	1033:1060	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	%					955:955	3%	954:955	3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG	954:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	BP					1029:1030	fine BP	1024:1030	fine BP	1024:1030	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	cellulose					984:992	cellulose	984:992	cellulose (CEL)	984:998	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	MG					1058:1059	MG	1058:1059	MG	1058:1059	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	pulp					1013:1016	coarse beet pulp	1001:1016	coarse beet pulp (BP)	1001:1021	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	CEL					995:997	CEL	995:997	CEL	995:997	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	sepiolite					967:975	either sepiolite	960:975	either sepiolite (SEP)	960:981	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	BP					1019:1020	BP	1019:1020	BP	1019:1020	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	SEP					978:980	SEP	978:980	SEP	978:980	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	33	theme	sepiolite	967:975	arg1	MG					1072:1073	fine MG	1067:1073	fine MG	1067:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	2	34	theme	better	367:372	arg1	utilization					383:393	better nutrient utilization	367:393	better nutrient utilization	367:393	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	4	35	theme	process	695:701	arg1	residues					703:710	food process residues	690:710	food process residues	690:710	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	0	36	theme	fiber	10:14	arg1	source					16:21	fiber source	10:21	fiber source	10:21	Effect of fiber source and particle size on chick performance and nutrient utilization.					
31392330	3	37	theme	wide	466:469	arg1	fluctuation					471:481	wide fluctuation	466:481	wide fluctuation in their nutritional and chemical composition due to variation in processing	466:558	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31392330	10	38	theme	P	1578:1578	arg1	<					1580:1580	P < 0.05	1578:1585	P < 0.05	1578:1585	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	2	39	theme	chick	399:403	arg1	growth					405:410	chick growth	399:410	chick growth	399:410	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	3	40	theme	fiber	426:430	arg1	sources					432:438	The previous fiber sources	413:438	The previous fiber sources evaluated	413:448	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31392330	3	40	theme	fiber	426:430	arg1	subject					455:461	subject	455:461	subject	455:461	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31392330	0	41	theme	size	36:39	arg1	Effect					0:5	Effect	0:5	Effect of fiber source and particle size on chick performance and nutrient utilization.	0:86	Effect of fiber source and particle size on chick performance and nutrient utilization.					
31392330	6	42	theme	coarse	1001:1006	arg1	BP					1019:1020	BP	1019:1020	BP	1019:1020	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	42	theme	coarse	1001:1006	arg1	pulp					1013:1016	coarse beet pulp	1001:1016	coarse beet pulp (BP)	1001:1021	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	43	theme	coarse	1033:1038	arg1	grass					1051:1055	coarse Miscanthus grass	1033:1055	coarse Miscanthus grass (MG)	1033:1060	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	43	theme	coarse	1033:1038	arg1	MG					1058:1059	MG	1058:1059	MG	1058:1059	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	2	44	theme	fibers	293:298	arg1	inclusion					270:278	moderate inclusion	261:278	moderate inclusion of insoluble fibers (2 to 3%)	261:308	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	1	45	from	addition	92:99	arg1	chick					113:117	chick feeds	113:123	chick feeds	113:123	The addition of fiber in chick feeds is known to dilute nutrients; as a result, this may reduce nutrient digestibility and performance.					
31392330	10	46	theme	Particle	1473:1480	arg1	size					1482:1485	Particle size	1473:1485	Particle size of the fiber	1473:1498	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	1	47	theme	nutrient	184:191	arg1	result					160:165	a result	158:165	a result	158:165	The addition of fiber in chick feeds is known to dilute nutrients; as a result, this may reduce nutrient digestibility and performance.					
31392330	1	47	theme	nutrient	184:191	arg1	digestibility					193:205	nutrient digestibility	184:205	nutrient digestibility	184:205	The addition of fiber in chick feeds is known to dilute nutrients; as a result, this may reduce nutrient digestibility and performance.					
31392330	1	47	theme	nutrient	184:191	arg1	performance					211:221	performance	211:221	performance	211:221	The addition of fiber in chick feeds is known to dilute nutrients; as a result, this may reduce nutrient digestibility and performance.					
31392330	6	48	theme	Day-old	870:876	arg1	chicks					878:883	Day-old chicks	870:883	Day-old chicks (8 chicks per cage, 5 cages per treatment)	870:926	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	4	49	contain	has	643:645	arg2	composition					666:676	a consistent fiber composition	647:676	a consistent fiber composition	647:676	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	4	49	contain	has	643:645	arg1	grass					590:594	a C4 grass	585:594	a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues	585:710	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	4	49	contain	has	643:645	arg1	giganteus					572:580	Miscanthus giganteus	561:580	Miscanthus giganteus	561:580	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	11	50	theme	BP	1792:1793	arg1	diets					1795:1799	BP diets	1792:1799	BP diets	1792:1799	Birds fed diets containing MG performed similar to chicks fed CEL (P > 0.05), but digestibility coefficients of birds fed BP diets were generally higher than chicks fed MG diets.					
31392330	10	51	dep	higher	1570:1575	arg1	<					1580:1580	P < 0.05	1578:1585	P < 0.05	1578:1585	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	10	52	theme	fiber	1494:1498	arg1	size					1482:1485	Particle size	1473:1485	Particle size of the fiber	1473:1498	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	11	53	theme	birds	1782:1786	arg1	coefficients					1766:1777	digestibility coefficients	1752:1777	digestibility coefficients of birds fed BP diets	1752:1799	Birds fed diets containing MG performed similar to chicks fed CEL (P > 0.05), but digestibility coefficients of birds fed BP diets were generally higher than chicks fed MG diets.					
31392330	6	54	theme	BP	1029:1030	arg1	grass					1051:1055	coarse Miscanthus grass	1033:1055	coarse Miscanthus grass (MG)	1033:1060	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	%					955:955	3%	954:955	3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG	954:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	BP					1029:1030	fine BP	1024:1030	fine BP	1024:1030	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	cellulose					984:992	cellulose	984:992	cellulose (CEL)	984:998	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	MG					1058:1059	MG	1058:1059	MG	1058:1059	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	pulp					1013:1016	coarse beet pulp	1001:1016	coarse beet pulp (BP)	1001:1021	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	CEL					995:997	CEL	995:997	CEL	995:997	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	sepiolite					967:975	either sepiolite	960:975	either sepiolite (SEP)	960:981	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	BP					1019:1020	BP	1019:1020	BP	1019:1020	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	SEP					978:980	SEP	978:980	SEP	978:980	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	54	theme	BP	1029:1030	arg1	MG					1072:1073	fine MG	1067:1073	fine MG	1067:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	2	55	theme	recent	233:238	arg1	studies					240:246	recent studies	233:246	recent studies	233:246	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	1	56	theme	dilute	137:142	arg1	nutrients					144:152	dilute nutrients	137:152	dilute nutrients	137:152	The addition of fiber in chick feeds is known to dilute nutrients; as a result, this may reduce nutrient digestibility and performance.					
31392330	9	57	theme	feed	1423:1426	arg1	rate					1439:1442	better feed conversion rate	1416:1442	better feed conversion rate	1416:1442	In general, chicks fed diets containing fiber consumed more feed, gained more weight, and had better feed conversion rate than birds fed the SEP diet.					
31392330	6	58	theme	MG	1072:1073	arg1	grass					1051:1055	coarse Miscanthus grass	1033:1055	coarse Miscanthus grass (MG)	1033:1060	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	%					955:955	3%	954:955	3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG	954:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	BP					1029:1030	fine BP	1024:1030	fine BP	1024:1030	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	cellulose					984:992	cellulose	984:992	cellulose (CEL)	984:998	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	MG					1058:1059	MG	1058:1059	MG	1058:1059	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	pulp					1013:1016	coarse beet pulp	1001:1016	coarse beet pulp (BP)	1001:1021	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	CEL					995:997	CEL	995:997	CEL	995:997	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	sepiolite					967:975	either sepiolite	960:975	either sepiolite (SEP)	960:981	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	BP					1019:1020	BP	1019:1020	BP	1019:1020	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	SEP					978:980	SEP	978:980	SEP	978:980	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	58	theme	MG	1072:1073	arg1	MG					1072:1073	fine MG	1067:1073	fine MG	1067:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	3	59	theme	chemical	508:515	arg1	composition					517:527	their nutritional and chemical composition	486:527	their nutritional and chemical composition due to variation in processing	486:558	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31392330	5	60	theme	size	808:811	arg1	effect					764:769	the effect	760:769	the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility	760:867	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	7	61	theme	feed	1187:1190	arg1	intake					1192:1197	feed intake	1187:1197	feed intake	1187:1197	At the end of days 7, 14, and 21, chicks and experimental diets were weighed to compute average daily gain and feed intake.					
31392330	3	62	theme	nutritional	492:502	arg1	composition					517:527	their nutritional and chemical composition	486:527	their nutritional and chemical composition due to variation in processing	486:558	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31392330	6	63	theme	pulp	1013:1016	arg1	grass					1051:1055	coarse Miscanthus grass	1033:1055	coarse Miscanthus grass (MG)	1033:1060	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	%					955:955	3%	954:955	3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG	954:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	BP					1029:1030	fine BP	1024:1030	fine BP	1024:1030	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	cellulose					984:992	cellulose	984:992	cellulose (CEL)	984:998	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	MG					1058:1059	MG	1058:1059	MG	1058:1059	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	pulp					1013:1016	coarse beet pulp	1001:1016	coarse beet pulp (BP)	1001:1021	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	CEL					995:997	CEL	995:997	CEL	995:997	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	sepiolite					967:975	either sepiolite	960:975	either sepiolite (SEP)	960:981	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	BP					1019:1020	BP	1019:1020	BP	1019:1020	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	SEP					978:980	SEP	978:980	SEP	978:980	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	63	theme	pulp	1013:1016	arg1	MG					1072:1073	fine MG	1067:1073	fine MG	1067:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	7	64	theme	days	1090:1093	arg1	end					1083:1085	the end	1079:1085	the end of days 7, 14, and 21	1079:1107	At the end of days 7, 14, and 21, chicks and experimental diets were weighed to compute average daily gain and feed intake.					
31392330	5	65	theme	fiber	782:786	arg1	source					788:793	dietary fiber source	774:793	dietary fiber source	774:793	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	7	66	theme	average	1164:1170	arg1	gain					1178:1181	average daily gain	1164:1181	average daily gain	1164:1181	At the end of days 7, 14, and 21, chicks and experimental diets were weighed to compute average daily gain and feed intake.					
31392330	4	67	theme	C4	587:588	arg1	grass					590:594	a C4 grass	585:594	a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues	585:710	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	4	67	theme	C4	587:588	arg1	giganteus					572:580	Miscanthus giganteus	561:580	Miscanthus giganteus	561:580	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	3	68	theme	due	529:531	arg1	composition					517:527	their nutritional and chemical composition	486:527	their nutritional and chemical composition due to variation in processing	486:558	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31392330	5	69	theme	nutrient	846:853	arg1	digestibility					855:867	nutrient digestibility	846:867	nutrient digestibility	846:867	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	0	70	from	Effect	0:5	arg1	performance					50:60	chick performance	44:60	chick performance	44:60	Effect of fiber source and particle size on chick performance and nutrient utilization.					
31392330	0	70	from	Effect	0:5	arg1	utilization					75:85	nutrient utilization	66:85	nutrient utilization	66:85	Effect of fiber source and particle size on chick performance and nutrient utilization.					
31392330	11	71	dep	similar	1710:1716	arg1	>					1739:1739	P > 0.05	1737:1744	P > 0.05	1737:1744	Birds fed diets containing MG performed similar to chicks fed CEL (P > 0.05), but digestibility coefficients of birds fed BP diets were generally higher than chicks fed MG diets.					
31392330	2	72	theme	moderate	261:268	arg1	inclusion					270:278	moderate inclusion	261:278	moderate inclusion of insoluble fibers (2 to 3%)	261:308	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	6	73	theme	grass	1051:1055	arg1	grass					1051:1055	coarse Miscanthus grass	1033:1055	coarse Miscanthus grass (MG)	1033:1060	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	%					955:955	3%	954:955	3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG	954:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	BP					1029:1030	fine BP	1024:1030	fine BP	1024:1030	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	cellulose					984:992	cellulose	984:992	cellulose (CEL)	984:998	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	MG					1058:1059	MG	1058:1059	MG	1058:1059	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	pulp					1013:1016	coarse beet pulp	1001:1016	coarse beet pulp (BP)	1001:1021	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	CEL					995:997	CEL	995:997	CEL	995:997	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	sepiolite					967:975	either sepiolite	960:975	either sepiolite (SEP)	960:981	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	BP					1019:1020	BP	1019:1020	BP	1019:1020	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	SEP					978:980	SEP	978:980	SEP	978:980	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	73	theme	grass	1051:1055	arg1	MG					1072:1073	fine MG	1067:1073	fine MG	1067:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	4	74	theme	fiber	660:664	arg1	composition					666:676	a consistent fiber composition	647:676	a consistent fiber composition	647:676	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	10	75	theme	nutrient	1545:1552	arg1	utilization					1554:1564	nutrient utilization	1545:1564	nutrient utilization	1545:1564	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	5	76	theme	day-old	816:822	arg1	performance					830:840	day-old chick performance	816:840	day-old chick performance	816:840	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	6	77	theme	fed	933:935	arg1	diets					937:941	fed diets	933:941	fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG	933:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	8	78	theme	capture	1257:1263	arg1	point					1265:1269	each data capture point	1247:1269	each data capture point	1247:1269	In addition, excreta from the previous 48 h of each data capture point was collected to determine nutrient digestibility.					
31392330	7	79	theme	experimental	1121:1132	arg1	diets					1134:1138	experimental diets	1121:1138	experimental diets	1121:1138	At the end of days 7, 14, and 21, chicks and experimental diets were weighed to compute average daily gain and feed intake.					
31392330	4	80	theme	fiber	623:627	arg1	content					629:635	its fiber content	619:635	its fiber content	619:635	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	0	81	theme	nutrient	66:73	arg1	utilization					75:85	nutrient utilization	66:85	nutrient utilization	66:85	Effect of fiber source and particle size on chick performance and nutrient utilization.					
31392330	1	82	dep	chick	113:117	arg1	feeds					119:123	feeds	119:123	feeds	119:123	The addition of fiber in chick feeds is known to dilute nutrients; as a result, this may reduce nutrient digestibility and performance.					
31392330	2	83	theme	insoluble	283:291	arg1	%					307:307	2 to 3%	301:307	2 to 3%	301:307	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	2	83	theme	insoluble	283:291	arg1	fibers					293:298	insoluble fibers	283:298	insoluble fibers (2 to 3%)	283:308	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	8	84	from	48 h	1239:1242	arg1	excreta					1213:1219	excreta	1213:1219	excreta from the previous 48 h of each data capture point	1213:1269	In addition, excreta from the previous 48 h of each data capture point was collected to determine nutrient digestibility.					
31392330	5	85	from	effect	764:769	arg1	performance					830:840	day-old chick performance	816:840	day-old chick performance	816:840	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	5	85	from	effect	764:769	arg1	digestibility					855:867	nutrient digestibility	846:867	nutrient digestibility	846:867	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	8	86	theme	nutrient	1298:1305	arg1	digestibility					1307:1319	nutrient digestibility	1298:1319	nutrient digestibility	1298:1319	In addition, excreta from the previous 48 h of each data capture point was collected to determine nutrient digestibility.					
31392330	6	87	contain	containing	943:952	arg2	CEL					995:997	CEL	995:997	CEL	995:997	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	%					955:955	3%	954:955	3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG	954:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	pulp					1013:1016	coarse beet pulp	1001:1016	coarse beet pulp (BP)	1001:1021	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	SEP					978:980	SEP	978:980	SEP	978:980	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	BP					1019:1020	BP	1019:1020	BP	1019:1020	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg1	diets					937:941	fed diets	933:941	fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG	933:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	MG					1072:1073	fine MG	1067:1073	fine MG	1067:1073	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	grass					1051:1055	coarse Miscanthus grass	1033:1055	coarse Miscanthus grass (MG)	1033:1060	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	BP					1029:1030	fine BP	1024:1030	fine BP	1024:1030	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	MG					1058:1059	MG	1058:1059	MG	1058:1059	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	cellulose					984:992	cellulose	984:992	cellulose (CEL)	984:998	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	87	contain	containing	943:952	arg2	sepiolite					967:975	either sepiolite	960:975	either sepiolite (SEP)	960:981	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	2	88	theme	gizzard	324:330	arg1	development					332:342	gizzard development	324:342	gizzard development	324:342	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	10	89	theme	fine	1648:1651	arg1	particles					1659:1667	these fed fine fiber particles	1638:1667	these fed fine fiber particles	1638:1667	Particle size of the fiber had no effect on chick performance; however, nutrient utilization was higher (P < 0.05) for chicks fed coarse fiber particles compared to these fed fine fiber particles.					
31392330	2	90	theme	nutrient	374:381	arg1	utilization					383:393	better nutrient utilization	367:393	better nutrient utilization	367:393	However, recent studies suggest that moderate inclusion of insoluble fibers (2 to 3%) may stimulate gizzard development, which could result in better nutrient utilization and chick growth.					
31392330	0	91	theme	source	16:21	arg1	Effect					0:5	Effect	0:5	Effect of fiber source and particle size on chick performance and nutrient utilization.	0:86	Effect of fiber source and particle size on chick performance and nutrient utilization.					
31392330	4	92	theme	food	690:693	arg1	residues					703:710	food process residues	690:710	food process residues	690:710	Miscanthus giganteus is a C4 grass purposefully grown for its fiber content which has a consistent fiber composition compared to food process residues.					
31392330	3	93	from	variation	536:544	arg1	processing					549:558	processing	549:558	processing	549:558	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31392330	0	94	theme	particle	27:34	arg1	size					36:39	particle size	27:39	particle size	27:39	Effect of fiber source and particle size on chick performance and nutrient utilization.					
31392330	5	95	theme	study	736:740	arg1	objectives					717:726	The objectives	713:726	The objectives of this study	713:740	The objectives of this study were to determine the effect of dietary fiber source and particle size on day-old chick performance and nutrient digestibility.					
31392330	6	96	theme	beet	1008:1011	arg1	BP					1019:1020	BP	1019:1020	BP	1019:1020	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	6	96	theme	beet	1008:1011	arg1	pulp					1013:1016	coarse beet pulp	1001:1016	coarse beet pulp (BP)	1001:1021	Day-old chicks (8 chicks per cage, 5 cages per treatment) were fed diets containing 3% of either sepiolite (SEP), cellulose (CEL), coarse beet pulp (BP), fine BP, coarse Miscanthus grass (MG), and fine MG.					
31392330	3	97	theme	previous	417:424	arg1	sources					432:438	The previous fiber sources	413:438	The previous fiber sources evaluated	413:448	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31392330	3	97	theme	previous	417:424	arg1	subject					455:461	subject	455:461	subject	455:461	The previous fiber sources evaluated were subject to wide fluctuation in their nutritional and chemical composition due to variation in processing.					
31285029	5	0	theme	food	552:555	arg1	items					557:561	food items	552:561	food items out of the twenty proposed	552:588	A total of 120 participants had to compose dishes, following six different scenarios, by selecting three images of food items out of the twenty proposed.					
31285029	5	1	theme	items	557:561	arg1	images					542:547	three images	536:547	three images of food items out of the twenty proposed	536:588	A total of 120 participants had to compose dishes, following six different scenarios, by selecting three images of food items out of the twenty proposed.					
31285029	5	2	theme	different	502:510	arg1	scenarios					512:520	six different scenarios	498:520	six different scenarios	498:520	A total of 120 participants had to compose dishes, following six different scenarios, by selecting three images of food items out of the twenty proposed.					
31285029	1	3	theme	indirect	125:132	arg1	approach					134:141	an indirect approach	122:141	an indirect approach	122:141	Studying French consumers' representations of main dish structure, using an indirect approach.					
31285029	7	4	theme	food	846:849	arg1	products					851:858	animal-based food products	833:858	animal-based food products	833:858	Our results indicated that (1) French non-vegetarian consumers generally constructed their main dishes using animal-based food products first.					
31285029	12	5	theme	French	1354:1359	arg1	consumers					1361:1369	French consumers	1354:1369	French consumers	1354:1369	They also highlight possible levers that could be used to promote pulses to French consumers.					
31285029	11	6	theme	structure	1243:1251	arg1	evidence					1227:1234	new evidence	1223:1234	new evidence of the structure of the French main dish	1223:1275	These findings provide new evidence of the structure of the French main dish.					
31285029	11	7	theme	French	1260:1265	arg1	dish					1272:1275	the French main dish	1256:1275	the French main dish	1256:1275	These findings provide new evidence of the structure of the French main dish.					
31285029	4	8	theme	main	398:401	arg1	dish					403:406	their main dish	392:406	their main dish	392:406	In order to better understand it, this study seeks to identify how French non-vegetarian consumers structure their main dish, using an indirect approach.					
31285029	7	9	dep	consumers	777:785	arg1	1					752:752	1	752:752	1	752:752	Our results indicated that (1) French non-vegetarian consumers generally constructed their main dishes using animal-based food products first.					
31285029	6	10	dep	chosen	654:659	arg1	1					628:628	1	628:628	1	628:628	Results provided information about: (1) the first food product chosen, and (2) how foods from different food-groups were associated.					
31285029	6	10	dep	chosen	654:659	arg1	information					608:618	information	608:618	information about: (1) the first food product chosen, and (2)	608:668	Results provided information about: (1) the first food product chosen, and (2) how foods from different food-groups were associated.					
31285029	6	11	dep	provided	599:606	arg1	associated					712:721	associated	712:721	were associated	707:721	Results provided information about: (1) the first food product chosen, and (2) how foods from different food-groups were associated.					
31285029	0	12	theme	central	2:8	arg1	place					10:14	A central place	0:14	A central place for meat	0:23	A central place for meat, but what about pulses?					
31285029	9	13	dep	food-groups	1023:1033	arg1	2					1016:1016	2	1016:1016	2	1016:1016	Results also showed that (2) the food-groups most often associated within a dish were meat, starch, and vegetable.					
31285029	7	14	theme	main	815:818	arg1	dishes					820:825	their main dishes	809:825	their main dishes	809:825	Our results indicated that (1) French non-vegetarian consumers generally constructed their main dishes using animal-based food products first.					
31285029	1	15	theme	French	58:63	arg1	representations					76:90	French consumers' representations	58:90	French consumers' representations of main dish structure	58:113	Studying French consumers' representations of main dish structure, using an indirect approach.					
31285029	12	16	used	used	1328:1331	arg2	levers					1307:1312	possible levers	1298:1312	possible levers that could be used to promote pulses to French consumers	1298:1369	They also highlight possible levers that could be used to promote pulses to French consumers.					
31285029	10	17	dep	general	1140:1146	arg1	associated					1148:1157	associated	1148:1157	associated with the meat and vegetable food-groups	1148:1197	The pulse group, when used, was in general associated with the meat and vegetable food-groups.					
31285029	10	17	dep	general	1140:1146	arg1	group					1115:1119	The pulse group	1105:1119	The pulse group	1105:1119	The pulse group, when used, was in general associated with the meat and vegetable food-groups.					
31285029	7	18	theme	animal-based	833:844	arg1	products					851:858	animal-based food products	833:858	animal-based food products	833:858	Our results indicated that (1) French non-vegetarian consumers generally constructed their main dishes using animal-based food products first.					
31285029	3	19	theme	plant-based	230:240	arg1	pulses					260:265	pulses	260:265	pulses	260:265	The role played by plant-based proteins, such as pulses, is less clear.					
31285029	3	19	theme	plant-based	230:240	arg1	proteins					242:249	plant-based proteins	230:249	plant-based proteins	230:249	The role played by plant-based proteins, such as pulses, is less clear.					
31285029	11	20	theme	dish	1272:1275	arg1	structure					1243:1251	the structure	1239:1251	the structure of the French main dish	1239:1275	These findings provide new evidence of the structure of the French main dish.					
31285029	11	21	theme	main	1267:1270	arg1	dish					1272:1275	the French main dish	1256:1275	the French main dish	1256:1275	These findings provide new evidence of the structure of the French main dish.					
31285029	4	22	theme	non-vegetarian	357:370	arg1	consumers					372:380	French non-vegetarian consumers	350:380	French non-vegetarian consumers	350:380	In order to better understand it, this study seeks to identify how French non-vegetarian consumers structure their main dish, using an indirect approach.					
31285029	5	23	theme	participants	452:463	arg1	total					439:443	A total	437:443	A total of 120 participants	437:463	A total of 120 participants had to compose dishes, following six different scenarios, by selecting three images of food items out of the twenty proposed.					
31285029	8	24	theme	participants	976:987	arg1	relation					917:924	relation	917:924	relation to the scenario presented	917:950	Some differences were nevertheless identified, in relation to the scenario presented, and the profile of the participants.					
31285029	8	24	theme	participants	976:987	arg1	profile					961:967	the profile	957:967	the profile of the participants	957:987	Some differences were nevertheless identified, in relation to the scenario presented, and the profile of the participants.					
31285029	2	25	theme	central	175:181	arg1	place					183:187	a central place	173:187	a central place	173:187	Over time, meat has acquired a central place in French gastronomy.					
31285029	6	26	theme	food	641:644	arg1	product					646:652	the first food product	631:652	the first food product	631:652	Results provided information about: (1) the first food product chosen, and (2) how foods from different food-groups were associated.					
31285029	6	27	from	food-groups	695:705	arg1	foods					674:678	foods	674:678	foods from different food-groups	674:705	Results provided information about: (1) the first food product chosen, and (2) how foods from different food-groups were associated.					
31285029	12	28	theme	possible	1298:1305	arg1	levers					1307:1312	possible levers	1298:1312	possible levers that could be used to promote pulses to French consumers	1298:1369	They also highlight possible levers that could be used to promote pulses to French consumers.					
31285029	6	29	theme	first	635:639	arg1	product					646:652	the first food product	631:652	the first food product	631:652	Results provided information about: (1) the first food product chosen, and (2) how foods from different food-groups were associated.					
31285029	1	30	theme	main	95:98	arg1	structure					105:113	main dish structure	95:113	main dish structure	95:113	Studying French consumers' representations of main dish structure, using an indirect approach.					
31285029	7	31	theme	non-vegetarian	762:775	arg1	consumers					777:785	(1) French non-vegetarian consumers	751:785	(1) French non-vegetarian consumers	751:785	Our results indicated that (1) French non-vegetarian consumers generally constructed their main dishes using animal-based food products first.					
31285029	1	32	theme	dish	100:103	arg1	structure					105:113	main dish structure	95:113	main dish structure	95:113	Studying French consumers' representations of main dish structure, using an indirect approach.					
31285029	4	33	dep	understand	302:311	arg1	to					292:293	to	292:293	to	292:293	In order to better understand it, this study seeks to identify how French non-vegetarian consumers structure their main dish, using an indirect approach.					
31285029	4	34	theme	French	350:355	arg1	consumers					372:380	French non-vegetarian consumers	350:380	French non-vegetarian consumers	350:380	In order to better understand it, this study seeks to identify how French non-vegetarian consumers structure their main dish, using an indirect approach.					
31285029	10	35	theme	meat	1168:1171	arg1	food-groups					1187:1197	the meat and vegetable food-groups	1164:1197	the meat and vegetable food-groups	1164:1197	The pulse group, when used, was in general associated with the meat and vegetable food-groups.					
31285029	1	36	theme	structure	105:113	arg1	representations					76:90	French consumers' representations	58:90	French consumers' representations of main dish structure	58:113	Studying French consumers' representations of main dish structure, using an indirect approach.					
31285029	6	37	theme	different	685:693	arg1	food-groups					695:705	different food-groups	685:705	different food-groups	685:705	Results provided information about: (1) the first food product chosen, and (2) how foods from different food-groups were associated.					
31285029	7	38	theme	French	755:760	arg1	consumers					777:785	(1) French non-vegetarian consumers	751:785	(1) French non-vegetarian consumers	751:785	Our results indicated that (1) French non-vegetarian consumers generally constructed their main dishes using animal-based food products first.					
31285029	11	39	theme	new	1223:1225	arg1	evidence					1227:1234	new evidence	1223:1234	new evidence of the structure of the French main dish	1223:1275	These findings provide new evidence of the structure of the French main dish.					
31285029	4	40	theme	indirect	418:425	arg1	approach					427:434	an indirect approach	415:434	an indirect approach	415:434	In order to better understand it, this study seeks to identify how French non-vegetarian consumers structure their main dish, using an indirect approach.					
31285029	2	41	theme	French	192:197	arg1	gastronomy					199:208	French gastronomy	192:208	French gastronomy	192:208	Over time, meat has acquired a central place in French gastronomy.					
31285029	10	42	theme	vegetable	1177:1185	arg1	food-groups					1187:1197	the meat and vegetable food-groups	1164:1197	the meat and vegetable food-groups	1164:1197	The pulse group, when used, was in general associated with the meat and vegetable food-groups.					
31285029	10	43	theme	pulse	1109:1113	arg1	group					1115:1119	The pulse group	1105:1119	The pulse group	1105:1119	The pulse group, when used, was in general associated with the meat and vegetable food-groups.					
31285029	10	43	theme	pulse	1109:1113	arg1	associated					1148:1157	associated	1148:1157	associated with the meat and vegetable food-groups	1148:1197	The pulse group, when used, was in general associated with the meat and vegetable food-groups.					
29520715	9	0	theme	effects	1783:1789	arg1	functions					1758:1766	the functions	1754:1766	the functions of LeX and its effects in acute and chronic nervous system disease models	1754:1840	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	8	1	theme	neurite	1515:1521	arg1	outgrowth					1523:1531	neurite outgrowth	1515:1531	neurite outgrowth	1515:1531	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	9	2	theme	LeX	1771:1773	arg1	functions					1758:1766	the functions	1754:1766	the functions of LeX and its effects in acute and chronic nervous system disease models	1754:1840	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	1	3	theme	adhesion	169:176	arg1	molecules					178:186	adhesion molecules	169:186	adhesion molecules	169:186	Glycosylation fine-tunes signal transduction of adhesion molecules during neural development and supports synaptic plasticity and repair after injury in the adult nervous system.					
29520715	6	4	theme	antibody	1015:1022	arg1	binding					1024:1030	antibody binding	1015:1030	antibody binding to substrate-coated LeX glycomimetic peptide	1015:1075	In this assay, antibody binding to substrate-coated LeX glycomimetic peptide is measured in the presence of compounds, allowing identification of molecules that inhibit antibody binding and thereby mimic LeX.					
29520715	0	5	theme	Organic	74:80	arg1	Compounds					82:90	Novel Small Organic Compounds	62:90	Novel Small Organic Compounds Mimicking the LewisX Glycan	62:118	Enhanced Neuronal Survival and Neurite Outgrowth Triggered by Novel Small Organic Compounds Mimicking the LewisX Glycan.					
29520715	1	6	theme	molecules	178:186	arg1	transduction					153:164	signal transduction	146:164	signal transduction of adhesion molecules	146:186	Glycosylation fine-tunes signal transduction of adhesion molecules during neural development and supports synaptic plasticity and repair after injury in the adult nervous system.					
29520715	1	7	theme	adult	278:282	arg1	system					292:297	the adult nervous system	274:297	the adult nervous system	274:297	Glycosylation fine-tunes signal transduction of adhesion molecules during neural development and supports synaptic plasticity and repair after injury in the adult nervous system.					
29520715	6	8	theme	LeX	1052:1054	arg1	peptide					1069:1075	substrate-coated LeX glycomimetic peptide	1035:1075	substrate-coated LeX glycomimetic peptide	1035:1075	In this assay, antibody binding to substrate-coated LeX glycomimetic peptide is measured in the presence of compounds, allowing identification of molecules that inhibit antibody binding and thereby mimic LeX.					
29520715	9	9	from	functions	1758:1766	arg1	system					1820:1825	acute and chronic nervous system	1794:1825	acute and chronic nervous system	1794:1825	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	5	10	link	enzyme-linked	847:859	arg1	assay					875:879	competitive enzyme-linked immunosorbent assay	835:879	competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules	835:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	10	link	enzyme-linked	847:859	arg1	ELISA					882:886	ELISA	882:886	ELISA	882:886	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	4	11	theme	small	661:665	arg1	compounds					675:683	small organic compounds	661:683	small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules	661:795	To gain better insights into the functions of this glycan, we identified small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules.					
29520715	2	12	theme	expressed	315:323	arg1	glycan					332:337	One abundantly expressed neural glycan	300:337	One abundantly expressed neural glycan	300:337	One abundantly expressed neural glycan is LewisX (LeX).					
29520715	5	13	theme	small	975:979	arg1	molecules					989:997	small organic molecules	975:997	small organic molecules	975:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	1	14	theme	nervous	284:290	arg1	system					292:297	the adult nervous system	274:297	the adult nervous system	274:297	Glycosylation fine-tunes signal transduction of adhesion molecules during neural development and supports synaptic plasticity and repair after injury in the adult nervous system.					
29520715	7	15	theme	antibody	1282:1289	arg1	binding					1291:1297	antibody binding	1282:1297	antibody binding	1282:1297	Gossypol, orlistat, ursolic acid, folic acid, and tosufloxacin inhibited antibody binding in a concentration-dependent manner.					
29520715	9	16	theme	LeX	1697:1699	arg1	mimetics					1701:1708	these LeX mimetics	1691:1708	these LeX mimetics	1691:1708	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	10	17	theme	different	1973:1981	arg1	therapies					1983:1991	different therapies	1973:1991	different therapies	1973:1991	It is worth mentioning in this context that the LeX compounds investigated in the present study have been clinically approved for different therapies.					
29520715	5	18	theme	molecules	989:997	arg1	library					964:970	a library	962:970	a library of small organic molecules	962:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	9	19	theme	chronic	1804:1810	arg1	system					1820:1825	acute and chronic nervous system	1794:1825	acute and chronic nervous system	1794:1825	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	8	20	theme	neuronal	1545:1552	arg1	survival					1554:1561	neuronal survival	1545:1561	neuronal survival of cultured mouse cerebellar granule cells	1545:1604	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	9	21	theme	acute	1794:1798	arg1	system					1820:1825	acute and chronic nervous system	1794:1825	acute and chronic nervous system	1794:1825	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	5	22	theme	immunosorbent	861:873	arg1	assay					875:879	competitive enzyme-linked immunosorbent assay	835:879	competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules	835:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	22	theme	immunosorbent	861:873	arg1	ELISA					882:886	ELISA	882:886	ELISA	882:886	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	3	23	theme	postnatal	513:521	arg1	week					523:526	the first postnatal week	503:526	the first postnatal week	503:526	Although it is known that its expression starts at the formation of the neural tube during the second embryonic week in the mouse and peaks during the first postnatal week, its functional relevance is only rudimentarily understood.					
29520715	9	24	theme	disease	1827:1833	arg1	models					1835:1840	disease models	1827:1840	disease models	1827:1840	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	3	25	theme	tube	435:438	arg1	formation					411:419	the formation	407:419	the formation of the neural tube	407:438	Although it is known that its expression starts at the formation of the neural tube during the second embryonic week in the mouse and peaks during the first postnatal week, its functional relevance is only rudimentarily understood.					
29520715	0	26	theme	LewisX	106:111	arg1	Glycan					113:118	the LewisX Glycan	102:118	the LewisX Glycan	102:118	Enhanced Neuronal Survival and Neurite Outgrowth Triggered by Novel Small Organic Compounds Mimicking the LewisX Glycan.					
29520715	8	27	theme	cultured	1566:1573	arg1	cells					1600:1604	cultured mouse cerebellar granule cells	1566:1604	cultured mouse cerebellar granule cells	1566:1604	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	4	28	theme	neural	771:776	arg1	molecules					787:795	several neural adhesion molecules	763:795	several neural adhesion molecules	763:795	To gain better insights into the functions of this glycan, we identified small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules.					
29520715	6	29	theme	compounds	1108:1116	arg1	presence					1096:1103	the presence	1092:1103	the presence of compounds	1092:1116	In this assay, antibody binding to substrate-coated LeX glycomimetic peptide is measured in the presence of compounds, allowing identification of molecules that inhibit antibody binding and thereby mimic LeX.					
29520715	1	30	theme	neural	195:200	arg1	development					202:212	neural development	195:212	neural development	195:212	Glycosylation fine-tunes signal transduction of adhesion molecules during neural development and supports synaptic plasticity and repair after injury in the adult nervous system.					
29520715	0	31	theme	Neuronal	9:16	arg1	Survival					18:25	Enhanced Neuronal Survival	0:25	Enhanced Neuronal Survival	0:25	Enhanced Neuronal Survival and Neurite Outgrowth Triggered by Novel Small Organic Compounds Mimicking the LewisX Glycan.					
29520715	8	32	theme	granule	1592:1598	arg1	cells					1600:1604	cultured mouse cerebellar granule cells	1566:1604	cultured mouse cerebellar granule cells	1566:1604	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	10	33	theme	present	1925:1931	arg1	study					1933:1937	the present study	1921:1937	the present study	1921:1937	It is worth mentioning in this context that the LeX compounds investigated in the present study have been clinically approved for different therapies.					
29520715	7	34	theme	folic	1243:1247	arg1	acid					1249:1252	folic acid	1243:1252	folic acid	1243:1252	Gossypol, orlistat, ursolic acid, folic acid, and tosufloxacin inhibited antibody binding in a concentration-dependent manner.					
29520715	5	35	dep	antibodies	923:932	arg1	antibodies					923:932	the LeX-specific monoclonal antibodies	895:932	the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules	895:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	35	dep	antibodies	923:932	arg1	SSEA-1					941:946	SSEA-1	941:946	SSEA-1	941:946	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	35	dep	antibodies	923:932	arg1	L5					934:935	L5	934:935	L5	934:935	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	0	36	theme	Enhanced	0:7	arg1	Survival					18:25	Enhanced Neuronal Survival	0:25	Enhanced Neuronal Survival	0:25	Enhanced Neuronal Survival and Neurite Outgrowth Triggered by Novel Small Organic Compounds Mimicking the LewisX Glycan.					
29520715	8	37	theme	cerebellar	1581:1590	arg1	cells					1600:1604	cultured mouse cerebellar granule cells	1566:1604	cultured mouse cerebellar granule cells	1566:1604	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	9	38	theme	powerful	1718:1725	arg1	tools					1727:1731	powerful tools	1718:1731	powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models	1718:1840	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	9	39	from	effects	1783:1789	arg1	system					1820:1825	acute and chronic nervous system	1794:1825	acute and chronic nervous system	1794:1825	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	3	40	theme	neural	428:433	arg1	tube					435:438	the neural tube	424:438	the neural tube	424:438	Although it is known that its expression starts at the formation of the neural tube during the second embryonic week in the mouse and peaks during the first postnatal week, its functional relevance is only rudimentarily understood.					
29520715	8	41	theme	mouse	1575:1579	arg1	cells					1600:1604	cultured mouse cerebellar granule cells	1566:1604	cultured mouse cerebellar granule cells	1566:1604	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	9	42	from	LeX	1771:1773	arg1	system					1820:1825	acute and chronic nervous system	1794:1825	acute and chronic nervous system	1794:1825	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	3	43	theme	functional	533:542	arg1	relevance					544:552	its functional relevance	529:552	its functional relevance	529:552	Although it is known that its expression starts at the formation of the neural tube during the second embryonic week in the mouse and peaks during the first postnatal week, its functional relevance is only rudimentarily understood.					
29520715	3	44	theme	embryonic	458:466	arg1	week					468:471	the second embryonic week	447:471	the second embryonic week in the mouse	447:484	Although it is known that its expression starts at the formation of the neural tube during the second embryonic week in the mouse and peaks during the first postnatal week, its functional relevance is only rudimentarily understood.					
29520715	5	45	theme	competitive	835:845	arg1	assay					875:879	competitive enzyme-linked immunosorbent assay	835:879	competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules	835:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	45	theme	competitive	835:845	arg1	ELISA					882:886	ELISA	882:886	ELISA	882:886	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	8	46	theme	cells	1600:1604	arg1	survival					1554:1561	neuronal survival	1545:1561	neuronal survival of cultured mouse cerebellar granule cells	1545:1604	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	4	47	theme	adhesion	778:785	arg1	molecules					787:795	several neural adhesion molecules	763:795	several neural adhesion molecules	763:795	To gain better insights into the functions of this glycan, we identified small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules.					
29520715	8	48	theme	nM	1466:1467	arg1	concentrations					1469:1482	nM concentrations	1466:1482	nM concentrations	1466:1482	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	2	49	theme	neural	325:330	arg1	glycan					332:337	One abundantly expressed neural glycan	300:337	One abundantly expressed neural glycan	300:337	One abundantly expressed neural glycan is LewisX (LeX).					
29520715	1	50	theme	synaptic	227:234	arg1	plasticity					236:245	synaptic plasticity	227:245	synaptic plasticity	227:245	Glycosylation fine-tunes signal transduction of adhesion molecules during neural development and supports synaptic plasticity and repair after injury in the adult nervous system.					
29520715	5	51	theme	organic	981:987	arg1	molecules					989:997	small organic molecules	975:997	small organic molecules	975:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	7	52	theme	concentration-dependent	1304:1326	arg1	manner					1328:1333	a concentration-dependent manner	1302:1333	a concentration-dependent manner	1302:1333	Gossypol, orlistat, ursolic acid, folic acid, and tosufloxacin inhibited antibody binding in a concentration-dependent manner.					
29520715	0	53	theme	Neurite	31:37	arg1	Outgrowth					39:47	Neurite Outgrowth	31:47	Neurite Outgrowth	31:47	Enhanced Neuronal Survival and Neurite Outgrowth Triggered by Novel Small Organic Compounds Mimicking the LewisX Glycan.					
29520715	8	54	theme	actions	1423:1429	arg1	consequences					1392:1403	the molecular consequences	1378:1403	the molecular consequences of the compounds' actions	1378:1429	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	3	55	theme	second	451:456	arg1	week					468:471	the second embryonic week	447:471	the second embryonic week in the mouse	447:484	Although it is known that its expression starts at the formation of the neural tube during the second embryonic week in the mouse and peaks during the first postnatal week, its functional relevance is only rudimentarily understood.					
29520715	5	56	theme	Mimetic	798:804	arg1	compounds					806:814	Mimetic compounds	798:814	Mimetic compounds	798:814	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	57	theme	LeX-specific	899:910	arg1	antibodies					923:932	the LeX-specific monoclonal antibodies	895:932	the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules	895:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	57	theme	LeX-specific	899:910	arg1	SSEA-1					941:946	SSEA-1	941:946	SSEA-1	941:946	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	57	theme	LeX-specific	899:910	arg1	L5					934:935	L5	934:935	L5	934:935	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	6	58	theme	glycomimetic	1056:1067	arg1	peptide					1069:1075	substrate-coated LeX glycomimetic peptide	1035:1075	substrate-coated LeX glycomimetic peptide	1035:1075	In this assay, antibody binding to substrate-coated LeX glycomimetic peptide is measured in the presence of compounds, allowing identification of molecules that inhibit antibody binding and thereby mimic LeX.					
29520715	9	59	from	system	1820:1825	arg1	functions					1758:1766	the functions	1754:1766	the functions of LeX and its effects in acute and chronic nervous system disease models	1754:1840	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	8	60	theme	distinct	1620:1627	arg1	pathways					1649:1656	distinct signal transduction pathways	1620:1656	distinct signal transduction pathways	1620:1656	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	8	61	theme	mimetic	1489:1495	arg1	compounds					1497:1505	the mimetic compounds	1485:1505	the mimetic compounds	1485:1505	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	9	62	theme	nervous	1812:1818	arg1	system					1820:1825	acute and chronic nervous system	1794:1825	acute and chronic nervous system	1794:1825	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	8	63	theme	signal	1629:1634	arg1	pathways					1649:1656	distinct signal transduction pathways	1620:1656	distinct signal transduction pathways	1620:1656	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	4	64	attach	linked	753:758	arg1	molecules					787:795	several neural adhesion molecules	763:795	several neural adhesion molecules	763:795	To gain better insights into the functions of this glycan, we identified small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules.					
29520715	4	64	attach	linked	753:758	arg2	glycan					731:736	this glycan	726:736	this glycan glycosidically linked to several neural adhesion molecules	726:795	To gain better insights into the functions of this glycan, we identified small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules.					
29520715	9	65	dep	LeX	1771:1773	arg1	models					1835:1840	disease models	1827:1840	disease models	1827:1840	These findings raise hopes that these LeX mimetics will be powerful tools for further studying the functions of LeX and its effects in acute and chronic nervous system disease models.					
29520715	3	66	from	week	468:471	arg1	mouse					480:484	the mouse	476:484	the mouse	476:484	Although it is known that its expression starts at the formation of the neural tube during the second embryonic week in the mouse and peaks during the first postnatal week, its functional relevance is only rudimentarily understood.					
29520715	6	67	theme	molecules	1146:1154	arg1	identification					1128:1141	identification	1128:1141	identification of molecules that inhibit antibody binding and thereby mimic LeX	1128:1206	In this assay, antibody binding to substrate-coated LeX glycomimetic peptide is measured in the presence of compounds, allowing identification of molecules that inhibit antibody binding and thereby mimic LeX.					
29520715	4	68	theme	better	596:601	arg1	insights					603:610	better insights	596:610	better insights into the functions of this glycan	596:644	To gain better insights into the functions of this glycan, we identified small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules.					
29520715	8	69	theme	molecular	1382:1390	arg1	consequences					1392:1403	the molecular consequences	1378:1403	the molecular consequences of the compounds' actions	1378:1429	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	10	70	theme	LeX	1891:1893	arg1	compounds					1895:1903	the LeX compounds	1887:1903	the LeX compounds investigated in the present study	1887:1937	It is worth mentioning in this context that the LeX compounds investigated in the present study have been clinically approved for different therapies.					
29520715	10	70	theme	LeX	1891:1893	arg1	approved					1960:1967	approved	1960:1967	approved	1960:1967	It is worth mentioning in this context that the LeX compounds investigated in the present study have been clinically approved for different therapies.					
29520715	4	71	theme	several	763:769	arg1	molecules					787:795	several neural adhesion molecules	763:795	several neural adhesion molecules	763:795	To gain better insights into the functions of this glycan, we identified small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules.					
29520715	1	72	theme	signal	146:151	arg1	transduction					153:164	signal transduction	146:164	signal transduction of adhesion molecules	146:186	Glycosylation fine-tunes signal transduction of adhesion molecules during neural development and supports synaptic plasticity and repair after injury in the adult nervous system.					
29520715	6	73	theme	substrate-coated	1035:1050	arg1	peptide					1069:1075	substrate-coated LeX glycomimetic peptide	1035:1075	substrate-coated LeX glycomimetic peptide	1035:1075	In this assay, antibody binding to substrate-coated LeX glycomimetic peptide is measured in the presence of compounds, allowing identification of molecules that inhibit antibody binding and thereby mimic LeX.					
29520715	0	74	theme	Small	68:72	arg1	Compounds					82:90	Novel Small Organic Compounds	62:90	Novel Small Organic Compounds Mimicking the LewisX Glycan	62:118	Enhanced Neuronal Survival and Neurite Outgrowth Triggered by Novel Small Organic Compounds Mimicking the LewisX Glycan.					
29520715	5	75	theme	monoclonal	912:921	arg1	antibodies					923:932	the LeX-specific monoclonal antibodies	895:932	the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules	895:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	75	theme	monoclonal	912:921	arg1	SSEA-1					941:946	SSEA-1	941:946	SSEA-1	941:946	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	75	theme	monoclonal	912:921	arg1	L5					934:935	L5	934:935	L5	934:935	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	6	76	dep	inhibit	1161:1167	arg1	mimic					1198:1202	mimic	1198:1202	mimic LeX	1198:1206	In this assay, antibody binding to substrate-coated LeX glycomimetic peptide is measured in the presence of compounds, allowing identification of molecules that inhibit antibody binding and thereby mimic LeX.					
29520715	5	77	theme	enzyme-linked	847:859	arg1	assay					875:879	competitive enzyme-linked immunosorbent assay	835:879	competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules	835:997	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	5	77	theme	enzyme-linked	847:859	arg1	ELISA					882:886	ELISA	882:886	ELISA	882:886	Mimetic compounds were identified by competitive enzyme-linked immunosorbent assay (ELISA) using the LeX-specific monoclonal antibodies L5 and SSEA-1 for screening a library of small organic molecules.					
29520715	4	78	theme	organic	667:673	arg1	compounds					675:683	small organic compounds	661:683	small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules	661:795	To gain better insights into the functions of this glycan, we identified small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules.					
29520715	4	79	theme	glycan	639:644	arg1	functions					621:629	the functions	617:629	the functions of this glycan	617:644	To gain better insights into the functions of this glycan, we identified small organic compounds that mimic structurally and functionally this glycan glycosidically linked to several neural adhesion molecules.					
29520715	0	80	theme	Novel	62:66	arg1	Compounds					82:90	Novel Small Organic Compounds	62:90	Novel Small Organic Compounds Mimicking the LewisX Glycan	62:118	Enhanced Neuronal Survival and Neurite Outgrowth Triggered by Novel Small Organic Compounds Mimicking the LewisX Glycan.					
29520715	6	81	theme	antibody	1169:1176	arg1	binding					1178:1184	antibody binding	1169:1184	antibody binding	1169:1184	In this assay, antibody binding to substrate-coated LeX glycomimetic peptide is measured in the presence of compounds, allowing identification of molecules that inhibit antibody binding and thereby mimic LeX.					
29520715	8	82	theme	transduction	1636:1647	arg1	pathways					1649:1656	distinct signal transduction pathways	1620:1656	distinct signal transduction pathways	1620:1656	With the aim to functionally characterize the molecular consequences of the compounds' actions, we here present evidence that, at nM concentrations, the mimetic compounds enhance neurite outgrowth and promote neuronal survival of cultured mouse cerebellar granule cells via, notably, distinct signal transduction pathways.					
29520715	3	83	theme	first	507:511	arg1	week					523:526	the first postnatal week	503:526	the first postnatal week	503:526	Although it is known that its expression starts at the formation of the neural tube during the second embryonic week in the mouse and peaks during the first postnatal week, its functional relevance is only rudimentarily understood.					
29520715	7	84	theme	ursolic	1229:1235	arg1	acid					1237:1240	ursolic acid	1229:1240	ursolic acid	1229:1240	Gossypol, orlistat, ursolic acid, folic acid, and tosufloxacin inhibited antibody binding in a concentration-dependent manner.					
29696546	2	0	theme	SO42-	450:454	arg1	605.0 mg/L					456:465	SO42- 605.0 mg/L	450:465	SO42- 605.0 mg/L	450:465	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	0	theme	SO42-	450:454	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	3	1	theme	pH	624:625	arg1	effect					614:619	the effect	610:619	the effect of pH on wastewater composition and effectiveness of nutrient sorption	610:690	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	2	theme	wastewater	630:639	arg1	composition					641:651	wastewater composition	630:651	wastewater composition	630:651	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	1	3	theme	removal	153:159	arg1	effectiveness					136:148	the effectiveness	132:148	the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW)	132:236	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	3	4	theme	nutrients	801:809	arg1	removal					790:796	percentage removal	779:796	percentage removal of nutrients from GW	779:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	0	5	theme	cultivation	79:89	arg1	wastewater					91:100	soilless plant cultivation wastewater	64:100	soilless plant cultivation wastewater	64:100	Hydrogel chitosan sorbent application for nutrient removal from soilless plant cultivation wastewater.					
29696546	3	6	theme	study	578:582	arg1	scope					565:569	The scope	561:569	The scope of the study	561:582	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	7	theme	percentage	779:788	arg1	removal					790:796	percentage removal	779:796	percentage removal of nutrients from GW	779:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	1	8	theme	non-cross-linked	244:259	arg1	CHs					271:273	CHs	271:273	CHs	271:273	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	1	8	theme	non-cross-linked	244:259	arg1	chitosan					261:268	non-cross-linked chitosan	244:268	non-cross-linked chitosan (CHs)	244:274	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	2	9	theme	Ca2+	482:485	arg1	545.0 mg/L					487:496	Ca2+ 545.0 mg/L	482:496	Ca2+ 545.0 mg/L	482:496	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	9	theme	Ca2+	482:485	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	3	10	theme	effect	614:619	arg1	analyses					693:700	analyses	693:700	analyses of nutrient sorption kinetics	693:730	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	10	theme	effect	614:619	arg1	determination					593:605	determination	593:605	determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption	593:690	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	10	theme	effect	614:619	arg1	analyses					737:744	analyses	737:744	analyses of the effect of sorbent dose on percentage removal of nutrients from GW	737:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	2	11	theme	113°	540:543	arg1	dH					545:546	hardness 113° dH	531:546	hardness 113° dH	531:546	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	11	theme	113°	540:543	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	4	12	from	%	849:849	arg1	GW					884:885	GW	884:885	GW	884:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	2	13	theme	hardness	531:538	arg1	dH					545:546	hardness 113° dH	531:546	hardness 113° dH	531:546	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	13	theme	hardness	531:538	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	1	14	from	effectiveness	136:148	arg1	GW					234:235	GW	234:235	GW	234:235	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	1	14	from	effectiveness	136:148	arg1	wastewaters					221:231	greenhouse wastewaters	210:231	greenhouse wastewaters (GW)	210:236	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	3	15	from	removal	790:796	arg1	analyses					693:700	analyses	693:700	analyses of nutrient sorption kinetics	693:730	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	15	from	removal	790:796	arg1	determination					593:605	determination	593:605	determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption	593:690	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	15	from	removal	790:796	arg1	analyses					737:744	analyses	737:744	analyses of the effect of sorbent dose on percentage removal of nutrients from GW	737:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	4	16	theme	N-NO3	873:877	arg1	N-NO3					873:877	N-NO3	873:877	N-NO3 from GW	873:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	16	theme	N-NO3	873:877	arg1	P-PO4					854:858	P-PO4	854:858	P-PO4	854:858	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	16	theme	N-NO3	873:877	arg1	%					868:868	76.7%	864:868	76.7% of N-NO3 from GW	864:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	16	theme	N-NO3	873:877	arg1	%					849:849	79.4%	845:849	79.4% of P-PO4	845:858	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	17	theme	P-PO4	854:858	arg1	N-NO3					873:877	N-NO3	873:877	N-NO3 from GW	873:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	17	theme	P-PO4	854:858	arg1	P-PO4					854:858	P-PO4	854:858	P-PO4	854:858	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	17	theme	P-PO4	854:858	arg1	%					868:868	76.7%	864:868	76.7% of N-NO3 from GW	864:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	17	theme	P-PO4	854:858	arg1	%					849:849	79.4%	845:849	79.4% of P-PO4	845:858	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	2	18	dep	parameters	403:412	arg1	dH					545:546	hardness 113° dH	531:546	hardness 113° dH	531:546	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	18	dep	parameters	403:412	arg1	0.9 mg/L					472:479	Cl- 0.9 mg/L	468:479	Cl- 0.9 mg/L	468:479	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	18	dep	parameters	403:412	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	18	dep	parameters	403:412	arg1	482.0 mg/L					519:528	K+ 482.0 mg/L	516:528	K+ 482.0 mg/L	516:528	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	18	dep	parameters	403:412	arg1	605.0 mg/L					456:465	SO42- 605.0 mg/L	450:465	SO42- 605.0 mg/L	450:465	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	18	dep	parameters	403:412	arg1	pH					553:554	pH 6.2	553:558	pH 6.2	553:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	18	dep	parameters	403:412	arg1	545.0 mg/L					487:496	Ca2+ 545.0 mg/L	482:496	Ca2+ 545.0 mg/L	482:496	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	18	dep	parameters	403:412	arg1	621.1 mg/L					421:430	N-NO3 621.1 mg/L	415:430	N-NO3 621.1 mg/L	415:430	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	18	dep	parameters	403:412	arg1	178.0 mg/L					504:513	Mg2+ 178.0 mg/L	499:513	Mg2+ 178.0 mg/L	499:513	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	18	dep	parameters	403:412	arg1	60.8 mg/L					439:447	P-PO4 60.8 mg/L	433:447	P-PO4 60.8 mg/L	433:447	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	1	19	link	non-cross-linked	244:259	arg1	CHs					271:273	CHs	271:273	CHs	271:273	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	1	19	link	non-cross-linked	244:259	arg1	chitosan					261:268	non-cross-linked chitosan	244:268	non-cross-linked chitosan (CHs)	244:274	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	1	20	theme	nutrients	164:172	arg1	removal					153:159	removal	153:159	removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW)	153:236	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	2	21	theme	Cl-	468:470	arg1	0.9 mg/L					472:479	Cl- 0.9 mg/L	468:479	Cl- 0.9 mg/L	468:479	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	21	theme	Cl-	468:470	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	1	22	theme	hydrogel	348:355	arg1	beads					357:361	hydrogel beads	348:361	hydrogel beads	348:361	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	4	23	dep	able	832:835	arg1	N-NO3					873:877	N-NO3	873:877	N-NO3 from GW	873:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	23	dep	able	832:835	arg1	P-PO4					854:858	P-PO4	854:858	P-PO4	854:858	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	23	dep	able	832:835	arg1	%					868:868	76.7%	864:868	76.7% of N-NO3 from GW	864:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	23	dep	able	832:835	arg1	%					849:849	79.4%	845:849	79.4% of P-PO4	845:858	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	2	24	theme	N-NO3	415:419	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	24	theme	N-NO3	415:419	arg1	621.1 mg/L					421:430	N-NO3 621.1 mg/L	415:430	N-NO3 621.1 mg/L	415:430	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	1	25	theme	beads	357:361	arg1	form					340:343	the form	336:343	the form of hydrogel beads	336:361	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	3	26	from	determination	593:605	arg1	removal					790:796	percentage removal	779:796	percentage removal of nutrients from GW	779:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	26	from	determination	593:605	arg1	effectiveness					657:669	effectiveness	657:669	effectiveness	657:669	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	26	from	determination	593:605	arg1	composition					641:651	wastewater composition	630:651	wastewater composition	630:651	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	0	27	theme	Hydrogel	0:7	arg1	chitosan					9:16	Hydrogel chitosan	0:16	Hydrogel chitosan	0:16	Hydrogel chitosan sorbent application for nutrient removal from soilless plant cultivation wastewater.					
29696546	3	28	theme	nutrient	674:681	arg1	sorption					683:690	nutrient sorption	674:690	nutrient sorption	674:690	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	29	from	effectiveness	657:669	arg1	analyses					693:700	analyses	693:700	analyses of nutrient sorption kinetics	693:730	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	29	from	effectiveness	657:669	arg1	determination					593:605	determination	593:605	determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption	593:690	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	29	from	effectiveness	657:669	arg1	analyses					737:744	analyses	737:744	analyses of the effect of sorbent dose on percentage removal of nutrients from GW	737:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	30	from	analyses	693:700	arg1	removal					790:796	percentage removal	779:796	percentage removal of nutrients from GW	779:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	30	from	analyses	693:700	arg1	effectiveness					657:669	effectiveness	657:669	effectiveness	657:669	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	30	from	analyses	693:700	arg1	composition					641:651	wastewater composition	630:651	wastewater composition	630:651	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	31	from	composition	641:651	arg1	analyses					693:700	analyses	693:700	analyses of nutrient sorption kinetics	693:730	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	31	from	composition	641:651	arg1	determination					593:605	determination	593:605	determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption	593:690	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	31	from	composition	641:651	arg1	analyses					737:744	analyses	737:744	analyses of the effect of sorbent dose on percentage removal of nutrients from GW	737:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	32	theme	sorption	683:690	arg1	effectiveness					657:669	effectiveness	657:669	effectiveness	657:669	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	32	theme	sorption	683:690	arg1	composition					641:651	wastewater composition	630:651	wastewater composition	630:651	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	33	from	analyses	737:744	arg1	removal					790:796	percentage removal	779:796	percentage removal of nutrients from GW	779:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	33	from	analyses	737:744	arg1	effectiveness					657:669	effectiveness	657:669	effectiveness	657:669	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	33	from	analyses	737:744	arg1	composition					641:651	wastewater composition	630:651	wastewater composition	630:651	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	4	34	theme	N-NO3	938:942	arg1	N-NO3					938:942	N-NO3	938:942	N-NO3	938:942	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	34	theme	N-NO3	938:942	arg1	P-PO4					919:923	P-PO4	919:923	P-PO4	919:923	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	34	theme	N-NO3	938:942	arg1	%					933:933	53.2%	929:933	53.2% of N-NO3	929:942	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	34	theme	N-NO3	938:942	arg1	%					914:914	92.8%	910:914	92.8% of P-PO4	910:923	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	2	35	theme	following	393:401	arg1	dH					545:546	hardness 113° dH	531:546	hardness 113° dH	531:546	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	35	theme	following	393:401	arg1	0.9 mg/L					472:479	Cl- 0.9 mg/L	468:479	Cl- 0.9 mg/L	468:479	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	35	theme	following	393:401	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	35	theme	following	393:401	arg1	482.0 mg/L					519:528	K+ 482.0 mg/L	516:528	K+ 482.0 mg/L	516:528	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	35	theme	following	393:401	arg1	605.0 mg/L					456:465	SO42- 605.0 mg/L	450:465	SO42- 605.0 mg/L	450:465	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	35	theme	following	393:401	arg1	pH					553:554	pH 6.2	553:558	pH 6.2	553:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	35	theme	following	393:401	arg1	545.0 mg/L					487:496	Ca2+ 545.0 mg/L	482:496	Ca2+ 545.0 mg/L	482:496	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	35	theme	following	393:401	arg1	621.1 mg/L					421:430	N-NO3 621.1 mg/L	415:430	N-NO3 621.1 mg/L	415:430	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	35	theme	following	393:401	arg1	178.0 mg/L					504:513	Mg2+ 178.0 mg/L	499:513	Mg2+ 178.0 mg/L	499:513	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	35	theme	following	393:401	arg1	60.8 mg/L					439:447	P-PO4 60.8 mg/L	433:447	P-PO4 60.8 mg/L	433:447	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	36	theme	Mg2+	499:502	arg1	178.0 mg/L					504:513	Mg2+ 178.0 mg/L	499:513	Mg2+ 178.0 mg/L	499:513	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	36	theme	Mg2+	499:502	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	0	37	from	wastewater	91:100	arg1	removal					51:57	nutrient removal	42:57	nutrient removal from soilless plant cultivation wastewater	42:100	Hydrogel chitosan sorbent application for nutrient removal from soilless plant cultivation wastewater.					
29696546	0	38	theme	nutrient	42:49	arg1	removal					51:57	nutrient removal	42:57	nutrient removal from soilless plant cultivation wastewater	42:100	Hydrogel chitosan sorbent application for nutrient removal from soilless plant cultivation wastewater.					
29696546	4	39	theme	P-PO4	919:923	arg1	N-NO3					938:942	N-NO3	938:942	N-NO3	938:942	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	39	theme	P-PO4	919:923	arg1	P-PO4					919:923	P-PO4	919:923	P-PO4	919:923	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	39	theme	P-PO4	919:923	arg1	%					933:933	53.2%	929:933	53.2% of N-NO3	929:942	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	39	theme	P-PO4	919:923	arg1	%					914:914	92.8%	910:914	92.8% of P-PO4	910:923	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	2	40	theme	P-PO4	433:437	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	40	theme	P-PO4	433:437	arg1	60.8 mg/L					439:447	P-PO4 60.8 mg/L	433:447	P-PO4 60.8 mg/L	433:447	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	3	41	from	effect	614:619	arg1	effectiveness					657:669	effectiveness	657:669	effectiveness	657:669	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	41	from	effect	614:619	arg1	composition					641:651	wastewater composition	630:651	wastewater composition	630:651	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	42	theme	effect	753:758	arg1	analyses					693:700	analyses	693:700	analyses of nutrient sorption kinetics	693:730	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	42	theme	effect	753:758	arg1	determination					593:605	determination	593:605	determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption	593:690	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	42	theme	effect	753:758	arg1	analyses					737:744	analyses	737:744	analyses of the effect of sorbent dose on percentage removal of nutrients from GW	737:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	4	43	dep	remove	903:908	arg1	CHs					896:898	CHs	896:898	CHs	896:898	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	1	44	theme	greenhouse	210:219	arg1	GW					234:235	GW	234:235	GW	234:235	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	1	44	theme	greenhouse	210:219	arg1	wastewaters					221:231	greenhouse wastewaters	210:231	greenhouse wastewaters (GW)	210:236	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	3	45	theme	nutrient	705:712	arg1	kinetics					723:730	nutrient sorption kinetics	705:730	nutrient sorption kinetics	705:730	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	1	46	dep	nutrients	164:172	arg1	nitrates					175:182	nitrates	175:182	nitrates	175:182	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	1	46	dep	nutrients	164:172	arg1	orthophosphates					188:202	orthophosphates	188:202	orthophosphates	188:202	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	1	47	from	wastewaters	221:231	arg1	removal					153:159	removal	153:159	removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW)	153:236	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	1	47	from	wastewaters	221:231	arg1	effectiveness					136:148	the effectiveness	132:148	the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW)	132:236	In this study, we determined the effectiveness of removal of nutrients (nitrates and orthophosphates) from greenhouse wastewaters (GW) using non-cross-linked chitosan (CHs) and chitosan cross-linked with epichlorohydrin (CHs-ECH) in the form of hydrogel beads.					
29696546	3	48	from	effect	753:758	arg1	removal					790:796	percentage removal	779:796	percentage removal of nutrients from GW	779:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	49	theme	dose	771:774	arg1	effect					753:758	the effect	749:758	the effect of sorbent dose on percentage removal of nutrients from GW	749:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	2	50	theme	K+	516:517	arg1	482.0 mg/L					519:528	K+ 482.0 mg/L	516:528	K+ 482.0 mg/L	516:528	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	50	theme	K+	516:517	arg1	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	621.1 mg/L					421:430	N-NO3 621.1 mg/L	415:430	N-NO3 621.1 mg/L	415:430	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	605.0 mg/L					456:465	SO42- 605.0 mg/L	450:465	SO42- 605.0 mg/L	450:465	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	60.8 mg/L					439:447	P-PO4 60.8 mg/L	433:447	P-PO4 60.8 mg/L	433:447	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	545.0 mg/L					487:496	Ca2+ 545.0 mg/L	482:496	Ca2+ 545.0 mg/L	482:496	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	0.9 mg/L					472:479	Cl- 0.9 mg/L	468:479	Cl- 0.9 mg/L	468:479	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	178.0 mg/L					504:513	Mg2+ 178.0 mg/L	499:513	Mg2+ 178.0 mg/L	499:513	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	parameters					403:412	the following parameters	389:412	the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2	389:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	dH					545:546	hardness 113° dH	531:546	hardness 113° dH	531:546	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	pH					553:554	pH 6.2	553:558	pH 6.2	553:558	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg2	482.0 mg/L					519:528	K+ 482.0 mg/L	516:528	K+ 482.0 mg/L	516:528	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	2	51	contain	had	385:387	arg1	GW					364:365	GW	364:365	GW used in the study	364:383	GW used in the study had the following parameters: N-NO3 621.1 mg/L, P-PO4 60.8 mg/L, SO42- 605.0 mg/L, Cl- 0.9 mg/L, Ca2+ 545.0 mg/L, Mg2+ 178.0 mg/L, K+ 482.0 mg/L, hardness 113° dH, and pH 6.2.					
29696546	3	52	from	GW	816:817	arg1	removal					790:796	percentage removal	779:796	percentage removal of nutrients from GW	779:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	4	53	from	%	868:868	arg1	GW					884:885	GW	884:885	GW	884:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	0	54	theme	plant	73:77	arg1	wastewater					91:100	soilless plant cultivation wastewater	64:100	soilless plant cultivation wastewater	64:100	Hydrogel chitosan sorbent application for nutrient removal from soilless plant cultivation wastewater.					
29696546	3	55	theme	sorbent	763:769	arg1	dose					771:774	sorbent dose	763:774	sorbent dose	763:774	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	56	theme	sorption	714:721	arg1	kinetics					723:730	nutrient sorption kinetics	705:730	nutrient sorption kinetics	705:730	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	0	57	theme	soilless	64:71	arg1	wastewater					91:100	soilless plant cultivation wastewater	64:100	soilless plant cultivation wastewater	64:100	Hydrogel chitosan sorbent application for nutrient removal from soilless plant cultivation wastewater.					
29696546	4	58	from	GW	884:885	arg1	N-NO3					873:877	N-NO3	873:877	N-NO3 from GW	873:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	58	from	GW	884:885	arg1	P-PO4					854:858	P-PO4	854:858	P-PO4	854:858	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	58	from	GW	884:885	arg1	%					868:868	76.7%	864:868	76.7% of N-NO3 from GW	864:885	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	4	58	from	GW	884:885	arg1	%					849:849	79.4%	845:849	79.4% of P-PO4	845:858	CHs-ECH was able to sorb 79.4% of P-PO4 and 76.7% of N-NO3 from GW, whereas CHs to remove 92.8% of P-PO4 and 53.2% of N-NO3.					
29696546	3	59	theme	kinetics	723:730	arg1	analyses					693:700	analyses	693:700	analyses of nutrient sorption kinetics	693:730	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	59	theme	kinetics	723:730	arg1	determination					593:605	determination	593:605	determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption	593:690	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
29696546	3	59	theme	kinetics	723:730	arg1	analyses					737:744	analyses	737:744	analyses of the effect of sorbent dose on percentage removal of nutrients from GW	737:817	The scope of the study included determination of the effect of pH on wastewater composition and effectiveness of nutrient sorption, analyses of nutrient sorption kinetics, and analyses of the effect of sorbent dose on percentage removal of nutrients from GW.					
30614079	0	0	theme	Caulobacter	92:102	arg1	crescentus					104:113	Caulobacter crescentus	92:113	Caulobacter crescentus	92:113	Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus.					
30614079	7	1	theme	LD-crosslinking	1317:1331	arg1	consequence					1302:1312	a structural consequence	1289:1312	a structural consequence of LD-crosslinking	1289:1331	We also found that lysozyme resistance is a structural consequence of LD-crosslinking.					
30614079	7	1	theme	LD-crosslinking	1317:1331	arg1	resistance					1275:1284	lysozyme resistance	1266:1284	lysozyme resistance	1266:1284	We also found that lysozyme resistance is a structural consequence of LD-crosslinking.					
30614079	4	2	theme	wall	733:736	arg1	composition					695:705	the composition	691:705	the composition of the peptidoglycan cell wall of stalks and cell bodies	691:762	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	5	3	theme	meso-diaminopimelic	999:1017	arg1	acid					1044:1047	meso-diaminopimelic acid-meso-diaminopimelic acid	999:1047	meso-diaminopimelic acid-meso-diaminopimelic acid	999:1047	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	4	4	theme	cell	728:731	arg1	wall					733:736	the peptidoglycan cell wall	710:736	the peptidoglycan cell wall of stalks and cell bodies	710:762	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	8	5	theme	obvious	1345:1351	arg1	pressure					1363:1370	no obvious selection pressure	1342:1370	no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus	1342:1430	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	5	6	dep	contained	850:858	arg1	whereas					941:947	whereas	941:947	whereas	941:947	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	8	7	theme	other	1496:1500	arg1	organisms					1502:1510	other organisms	1496:1510	other organisms	1496:1510	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	0	8	from	regions	64:70	arg1	crescentus					104:113	Caulobacter crescentus	92:113	Caulobacter crescentus	92:113	Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus.					
30614079	6	9	from	turnover	1224:1231	arg1	stalk					1240:1244	the stalk	1236:1244	the stalk	1236:1244	We determined that ldtD is dispensable for stalk elongation; rather, stalk LD-transpeptidation reflects an aging process associated with low peptidoglycan turnover in the stalk.					
30614079	4	10	theme	previous	608:615	arg1	observation					617:627	the previous observation that C. crescentus stalks are lysozyme-resistant	604:676	the previous observation that C. crescentus stalks are lysozyme-resistant	604:676	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	4	11	theme	stalks	741:746	arg1	wall					733:736	the peptidoglycan cell wall	710:736	the peptidoglycan cell wall of stalks and cell bodies	710:762	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	7	12	theme	lysozyme	1266:1273	arg1	consequence					1302:1312	a structural consequence	1289:1312	a structural consequence of LD-crosslinking	1289:1331	We also found that lysozyme resistance is a structural consequence of LD-crosslinking.					
30614079	7	12	theme	lysozyme	1266:1273	arg1	resistance					1275:1284	lysozyme resistance	1266:1284	lysozyme resistance	1266:1284	We also found that lysozyme resistance is a structural consequence of LD-crosslinking.					
30614079	4	13	from	differences	783:793	arg1	crosslinking					812:823	peptidoglycan crosslinking	798:823	peptidoglycan crosslinking	798:823	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	5	14	theme	acid-meso-diaminopimelic	1019:1042	arg1	acid					1044:1047	meso-diaminopimelic acid-meso-diaminopimelic acid	999:1047	meso-diaminopimelic acid-meso-diaminopimelic acid	999:1047	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	1	15	theme	evolutionary	183:194	arg1	pressures					196:204	evolutionary pressures	183:204	evolutionary pressures that have produced physiologically important morphologies	183:262	The diversity of cell shapes across the bacterial kingdom reflects evolutionary pressures that have produced physiologically important morphologies.					
30614079	7	16	theme	structural	1291:1300	arg1	consequence					1302:1312	a structural consequence	1289:1312	a structural consequence of LD-crosslinking	1289:1331	We also found that lysozyme resistance is a structural consequence of LD-crosslinking.					
30614079	7	16	theme	structural	1291:1300	arg1	resistance					1275:1284	lysozyme resistance	1266:1284	lysozyme resistance	1266:1284	We also found that lysozyme resistance is a structural consequence of LD-crosslinking.					
30614079	4	17	theme	peptidoglycan	798:810	arg1	crosslinking					812:823	peptidoglycan crosslinking	798:823	peptidoglycan crosslinking	798:823	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	8	18	theme	lysozyme	1395:1402	arg1	resistance					1404:1413	LD-crosslinking or lysozyme resistance	1376:1413	LD-crosslinking or lysozyme resistance in C. crescentus	1376:1430	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	3	19	theme	polar	475:479	arg1	synthesis					487:495	polar stalk synthesis	475:495	polar stalk synthesis	475:495	For Caulobacter crescentus, polar stalk synthesis is tied to its dimorphic life cycle, and stalk elongation is regulated by phosphate availability.					
30614079	4	20	theme	key	779:781	arg1	differences					783:793	key differences	779:793	key differences in peptidoglycan crosslinking	779:823	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	3	21	theme	Caulobacter	451:461	arg1	crescentus					463:472	Caulobacter crescentus	451:472	Caulobacter crescentus	451:472	For Caulobacter crescentus, polar stalk synthesis is tied to its dimorphic life cycle, and stalk elongation is regulated by phosphate availability.					
30614079	0	22	theme	Differential	0:11	arg1	modes					13:17	Differential modes	0:17	Differential modes of crosslinking	0:33	Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus.					
30614079	8	23	theme	selection	1353:1361	arg1	pressure					1363:1370	no obvious selection pressure	1342:1370	no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus	1342:1430	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	2	24	theme	morphologies	349:360	arg1	regulation					312:321	the regulation	308:321	the regulation of some prototypical cell morphologies such as that of rod-shaped Escherichia coli	308:404	While efforts have been made to understand the regulation of some prototypical cell morphologies such as that of rod-shaped Escherichia coli, little is known about most cell shapes.					
30614079	8	25	theme	LD-crosslinking	1376:1390	arg1	resistance					1404:1413	LD-crosslinking or lysozyme resistance	1376:1413	LD-crosslinking or lysozyme resistance in C. crescentus	1376:1430	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	5	26	theme	stalk	949:953	arg1	peptidoglycan					955:967	stalk peptidoglycan	949:967	stalk peptidoglycan	949:967	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	0	27	theme	crosslinking	22:33	arg1	modes					13:17	Differential modes	0:17	Differential modes of crosslinking	0:33	Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus.					
30614079	3	28	theme	phosphate	571:579	arg1	availability					581:592	phosphate availability	571:592	phosphate availability	571:592	For Caulobacter crescentus, polar stalk synthesis is tied to its dimorphic life cycle, and stalk elongation is regulated by phosphate availability.					
30614079	5	29	theme	meso-diaminopimelic	892:910	arg1	acid					912:915	meso-diaminopimelic acid	892:915	meso-diaminopimelic acid	892:915	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	4	30	theme	bodies	757:762	arg1	wall					733:736	the peptidoglycan cell wall	710:736	the peptidoglycan cell wall of stalks and cell bodies	710:762	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	2	31	theme	prototypical	331:342	arg1	morphologies					349:360	some prototypical cell morphologies	326:360	some prototypical cell morphologies such as that of rod-shaped Escherichia coli	326:404	While efforts have been made to understand the regulation of some prototypical cell morphologies such as that of rod-shaped Escherichia coli, little is known about most cell shapes.					
30614079	2	31	theme	prototypical	331:342	arg1	that					370:373	that	370:373	that	370:373	While efforts have been made to understand the regulation of some prototypical cell morphologies such as that of rod-shaped Escherichia coli, little is known about most cell shapes.					
30614079	6	32	theme	stalk	1138:1142	arg1	LD-transpeptidation					1144:1162	stalk LD-transpeptidation	1138:1162	stalk LD-transpeptidation	1138:1162	We determined that ldtD is dispensable for stalk elongation; rather, stalk LD-transpeptidation reflects an aging process associated with low peptidoglycan turnover in the stalk.					
30614079	3	33	theme	life	522:525	arg1	cycle					527:531	its dimorphic life cycle	508:531	its dimorphic life cycle	508:531	For Caulobacter crescentus, polar stalk synthesis is tied to its dimorphic life cycle, and stalk elongation is regulated by phosphate availability.					
30614079	3	34	theme	stalk	481:485	arg1	synthesis					487:495	polar stalk synthesis	475:495	polar stalk synthesis	475:495	For Caulobacter crescentus, polar stalk synthesis is tied to its dimorphic life cycle, and stalk elongation is regulated by phosphate availability.					
30614079	1	35	theme	important	241:249	arg1	morphologies					251:262	physiologically important morphologies	225:262	physiologically important morphologies	225:262	The diversity of cell shapes across the bacterial kingdom reflects evolutionary pressures that have produced physiologically important morphologies.					
30614079	2	36	theme	most	429:432	arg1	shapes					439:444	most cell shapes	429:444	most cell shapes	429:444	While efforts have been made to understand the regulation of some prototypical cell morphologies such as that of rod-shaped Escherichia coli, little is known about most cell shapes.					
30614079	5	37	theme	Cell	826:829	arg1	peptidoglycan					836:848	Cell body peptidoglycan	826:848	Cell body peptidoglycan	826:848	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	8	38	theme	bacterial	1592:1600	arg1	sensitivity					1602:1612	bacterial sensitivity	1592:1612	bacterial sensitivity to lysozyme	1592:1624	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	2	39	theme	cell	434:437	arg1	shapes					439:444	most cell shapes	429:444	most cell shapes	429:444	While efforts have been made to understand the regulation of some prototypical cell morphologies such as that of rod-shaped Escherichia coli, little is known about most cell shapes.					
30614079	5	40	theme	body	831:834	arg1	peptidoglycan					836:848	Cell body peptidoglycan	826:848	Cell body peptidoglycan	826:848	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	5	41	contain	contained	850:858	arg1	peptidoglycan					836:848	Cell body peptidoglycan	826:848	Cell body peptidoglycan	826:848	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	5	41	contain	contained	850:858	arg2	DD-crosslinks					870:882	DD-crosslinks	870:882	DD-crosslinks between meso-diaminopimelic acid and D-alanine residues	870:938	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	5	42	dep	LD-transpeptidation	978:996	arg1	mediated					1051:1058	mediated	1051:1058	mediated by LdtD	1051:1066	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	5	42	dep	LD-transpeptidation	978:996	arg1	acid					1044:1047	meso-diaminopimelic acid-meso-diaminopimelic acid	999:1047	meso-diaminopimelic acid-meso-diaminopimelic acid	999:1047	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	3	43	theme	dimorphic	512:520	arg1	cycle					527:531	its dimorphic life cycle	508:531	its dimorphic life cycle	508:531	For Caulobacter crescentus, polar stalk synthesis is tied to its dimorphic life cycle, and stalk elongation is regulated by phosphate availability.					
30614079	0	44	theme	distinct	55:62	arg1	regions					64:70	spatially distinct regions	45:70	spatially distinct regions of peptidoglycan in Caulobacter crescentus	45:113	Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus.					
30614079	8	45	theme	DAP-DAP	1529:1535	arg1	mechanism					1567:1575	a general mechanism	1557:1575	a general mechanism for regulating bacterial sensitivity to lysozyme	1557:1624	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	8	45	theme	DAP-DAP	1529:1535	arg1	crosslinking					1537:1548	DAP-DAP crosslinking	1529:1548	DAP-DAP crosslinking	1529:1548	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	1	46	theme	cell	133:136	arg1	shapes					138:143	cell shapes	133:143	cell shapes	133:143	The diversity of cell shapes across the bacterial kingdom reflects evolutionary pressures that have produced physiologically important morphologies.					
30614079	1	47	theme	shapes	138:143	arg1	diversity					120:128	The diversity	116:128	The diversity of cell shapes across the bacterial kingdom	116:172	The diversity of cell shapes across the bacterial kingdom reflects evolutionary pressures that have produced physiologically important morphologies.					
30614079	2	48	theme	cell	344:347	arg1	morphologies					349:360	some prototypical cell morphologies	326:360	some prototypical cell morphologies such as that of rod-shaped Escherichia coli	326:404	While efforts have been made to understand the regulation of some prototypical cell morphologies such as that of rod-shaped Escherichia coli, little is known about most cell shapes.					
30614079	2	48	theme	cell	344:347	arg1	that					370:373	that	370:373	that	370:373	While efforts have been made to understand the regulation of some prototypical cell morphologies such as that of rod-shaped Escherichia coli, little is known about most cell shapes.					
30614079	4	49	theme	C.	634:635	arg1	stalks					648:653	C. crescentus stalks	634:653	C. crescentus stalks	634:653	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	8	50	from	resistance	1404:1413	arg1	crescentus					1421:1430	C. crescentus	1418:1430	C. crescentus	1418:1430	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	1	51	theme	bacterial	156:164	arg1	kingdom					166:172	the bacterial kingdom	152:172	the bacterial kingdom	152:172	The diversity of cell shapes across the bacterial kingdom reflects evolutionary pressures that have produced physiologically important morphologies.					
30614079	4	52	theme	cell	752:755	arg1	bodies					757:762	cell bodies	752:762	cell bodies	752:762	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	0	53	theme	peptidoglycan	75:87	arg1	regions					64:70	spatially distinct regions	45:70	spatially distinct regions of peptidoglycan in Caulobacter crescentus	45:113	Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus.					
30614079	6	54	theme	low	1206:1208	arg1	turnover					1224:1231	low peptidoglycan turnover	1206:1231	low peptidoglycan turnover in the stalk	1206:1244	We determined that ldtD is dispensable for stalk elongation; rather, stalk LD-transpeptidation reflects an aging process associated with low peptidoglycan turnover in the stalk.					
30614079	8	55	theme	general	1559:1565	arg1	mechanism					1567:1575	a general mechanism	1557:1575	a general mechanism for regulating bacterial sensitivity to lysozyme	1557:1624	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	8	55	theme	general	1559:1565	arg1	crosslinking					1537:1548	DAP-DAP crosslinking	1529:1548	DAP-DAP crosslinking	1529:1548	Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.					
30614079	4	56	theme	peptidoglycan	714:726	arg1	wall					733:736	the peptidoglycan cell wall	710:736	the peptidoglycan cell wall of stalks and cell bodies	710:762	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30614079	6	57	theme	aging	1176:1180	arg1	process					1182:1188	an aging process	1173:1188	an aging process associated with low peptidoglycan turnover in the stalk	1173:1244	We determined that ldtD is dispensable for stalk elongation; rather, stalk LD-transpeptidation reflects an aging process associated with low peptidoglycan turnover in the stalk.					
30614079	6	58	theme	stalk	1112:1116	arg1	elongation					1118:1127	stalk elongation	1112:1127	stalk elongation	1112:1127	We determined that ldtD is dispensable for stalk elongation; rather, stalk LD-transpeptidation reflects an aging process associated with low peptidoglycan turnover in the stalk.					
30614079	6	59	theme	peptidoglycan	1210:1222	arg1	turnover					1224:1231	low peptidoglycan turnover	1206:1231	low peptidoglycan turnover in the stalk	1206:1244	We determined that ldtD is dispensable for stalk elongation; rather, stalk LD-transpeptidation reflects an aging process associated with low peptidoglycan turnover in the stalk.					
30614079	5	60	dep	acid	912:915	arg1	residues					931:938	residues	931:938	residues	931:938	Cell body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD.					
30614079	3	61	theme	stalk	538:542	arg1	elongation					544:553	stalk elongation	538:553	stalk elongation	538:553	For Caulobacter crescentus, polar stalk synthesis is tied to its dimorphic life cycle, and stalk elongation is regulated by phosphate availability.					
30614079	4	62	theme	crescentus	637:646	arg1	stalks					648:653	C. crescentus stalks	634:653	C. crescentus stalks	634:653	Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking.					
30579104	0	0	theme	Agave	77:81	arg1	bagasse					93:99	Agave tequilana bagasse	77:99	Agave tequilana bagasse	77:99	Mild reaction conditions induce high sugar yields during the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate.					
30579104	4	1	theme	solid	661:665	arg1	recovery					667:674	solid recovery	661:674	solid recovery after pretreatment with [Emim][OAc]	661:710	It was also revealed that solid recovery after pretreatment with [Emim][OAc] is a key factor.					
30579104	4	1	theme	solid	661:665	arg1	factor					721:726	a key factor	715:726	a key factor	715:726	It was also revealed that solid recovery after pretreatment with [Emim][OAc] is a key factor.					
30579104	6	2	from	xylose	990:995	arg1	hydrolysate					1004:1014	the hydrolysate	1000:1014	the hydrolysate	1000:1014	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	6	3	theme	untreated	1053:1061	arg1	ATB					1063:1065	untreated ATB	1053:1065	untreated ATB	1053:1065	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	6	4	theme	glucose	978:984	arg1	Yields					968:973	Yields	968:973	Yields of glucose and xylose in the hydrolysate	968:1014	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	5	5	from	increase	733:740	arg1	digestibility					755:767	enzymatic digestibility	745:767	enzymatic digestibility of pretreated ATB	745:785	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	1	6	theme	composite	179:187	arg1	designs					189:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	0	7	theme	bagasse	93:99	arg1	pretreatment					61:72	the pretreatment	57:72	the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate	57:140	Mild reaction conditions induce high sugar yields during the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate.					
30579104	1	8	dep	[Emim	308:312	arg1	[OAc					314:317	[OAc	314:317	[OAc	314:317	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	1	9	theme	1-ethyl-3-methylimidazolium	271:297	arg1	[Emim					308:312	[Emim	308:312	[Emim	308:312	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	1	9	theme	1-ethyl-3-methylimidazolium	271:297	arg1	acetate					299:305	1-ethyl-3-methylimidazolium acetate	271:305	1-ethyl-3-methylimidazolium acetate ([Emim][OAc])	271:319	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	0	10	theme	tequilana	83:91	arg1	bagasse					93:99	Agave tequilana bagasse	77:99	Agave tequilana bagasse	77:99	Mild reaction conditions induce high sugar yields during the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate.					
30579104	6	11	theme	%	1128:1128	arg1	xylan					1103:1107	xylan	1103:1107	xylan	1103:1107	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	6	11	theme	%	1128:1128	arg1	glucan					1092:1097	glucan	1092:1097	glucan	1092:1097	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	0	12	with	pretreatment	61:72	arg1	acetate					134:140	1-ethyl-3-methylimidazolium acetate	106:140	1-ethyl-3-methylimidazolium acetate	106:140	Mild reaction conditions induce high sugar yields during the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate.					
30579104	4	13	theme	key	717:719	arg1	recovery					667:674	solid recovery	661:674	solid recovery after pretreatment with [Emim][OAc]	661:710	It was also revealed that solid recovery after pretreatment with [Emim][OAc] is a key factor.					
30579104	4	13	theme	key	717:719	arg1	factor					721:726	a key factor	715:726	a key factor	715:726	It was also revealed that solid recovery after pretreatment with [Emim][OAc] is a key factor.					
30579104	5	14	dep	transform	935:943	arg1	infrared					945:952	infrared	945:952	transform infrared spectroscopy	935:965	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	0	15	theme	1-ethyl-3-methylimidazolium	106:132	arg1	acetate					134:140	1-ethyl-3-methylimidazolium acetate	106:140	1-ethyl-3-methylimidazolium acetate	106:140	Mild reaction conditions induce high sugar yields during the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate.					
30579104	6	16	from	Yields	968:973	arg1	hydrolysate					1004:1014	the hydrolysate	1000:1014	the hydrolysate	1000:1014	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	2	17	theme	sugar	399:403	arg1	variable					428:435	the response variable	415:435	the response variable	415:435	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	2	17	theme	sugar	399:403	arg1	yield					405:409	sugar yield	399:409	sugar yield	399:409	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	5	18	theme	pretreated	772:781	arg1	ATB					783:785	pretreated ATB	772:785	pretreated ATB	772:785	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	6	19	dep	glucan	1092:1097	arg1	conversions					1109:1119	conversions	1109:1119	conversions	1109:1119	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	0	20	theme	reaction	5:12	arg1	conditions					14:23	Mild reaction conditions	0:23	Mild reaction conditions	0:23	Mild reaction conditions induce high sugar yields during the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate.					
30579104	6	21	from	glucose	978:984	arg1	hydrolysate					1004:1014	the hydrolysate	1000:1014	the hydrolysate	1000:1014	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	6	22	from	hydrolysate	1004:1014	arg1	Yields					968:973	Yields	968:973	Yields of glucose and xylose in the hydrolysate	968:1014	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	2	23	theme	Reaction	322:329	arg1	temperature					337:347	temperature	337:347	temperature	337:347	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	2	23	theme	Reaction	322:329	arg1	time					331:334	Reaction time	322:334	Reaction time	322:334	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	2	23	theme	Reaction	322:329	arg1	factors					385:391	the studied factors	373:391	the studied factors	373:391	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	2	23	theme	Reaction	322:329	arg1	solids					353:358	solids	353:358	solids	353:358	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	0	24	theme	Mild	0:3	arg1	conditions					14:23	Mild reaction conditions	0:23	Mild reaction conditions	0:23	Mild reaction conditions induce high sugar yields during the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate.					
30579104	6	25	theme	%	1138:1138	arg1	xylan					1103:1107	xylan	1103:1107	xylan	1103:1107	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	6	25	theme	%	1138:1138	arg1	glucan					1092:1097	glucan	1092:1097	glucan	1092:1097	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	2	26	dep	time	331:334	arg1	loading					360:366	loading	360:366	loading	360:366	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	2	27	theme	studied	377:383	arg1	temperature					337:347	temperature	337:347	temperature	337:347	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	2	27	theme	studied	377:383	arg1	time					331:334	Reaction time	322:334	Reaction time	322:334	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	2	27	theme	studied	377:383	arg1	factors					385:391	the studied factors	373:391	the studied factors	373:391	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	2	27	theme	studied	377:383	arg1	solids					353:358	solids	353:358	solids	353:358	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	5	28	theme	ATB	783:785	arg1	digestibility					755:767	enzymatic digestibility	745:767	enzymatic digestibility of pretreated ATB	745:785	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	5	29	dep	Fourier	927:933	arg1	transform					935:943	transform	935:943	transform infrared spectroscopy	935:965	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	3	30	dep	solids	509:514	arg1	%					527:527	30%	525:527	30%	525:527	Results indicated that optimal conditions (119 °C, 142 min) using high solids loading (30%) were achieved at lower temperatures and reaction times than those previously reported in the literature.					
30579104	3	30	dep	solids	509:514	arg1	loading					516:522	loading	516:522	high solids loading (30%)	504:528	Results indicated that optimal conditions (119 °C, 142 min) using high solids loading (30%) were achieved at lower temperatures and reaction times than those previously reported in the literature.					
30579104	2	31	theme	response	419:426	arg1	variable					428:435	the response variable	415:435	the response variable	415:435	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	2	31	theme	response	419:426	arg1	yield					405:409	sugar yield	399:409	sugar yield	399:409	Reaction time, temperature and solids loading were the studied factors while sugar yield was the response variable.					
30579104	3	32	theme	lower	547:551	arg1	temperatures					553:564	lower temperatures	547:564	lower temperatures	547:564	Results indicated that optimal conditions (119 °C, 142 min) using high solids loading (30%) were achieved at lower temperatures and reaction times than those previously reported in the literature.					
30579104	5	33	theme	chemical	909:916	arg1	bonds					918:922	chemical bonds	909:922	chemical bonds	909:922	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	3	34	theme	high	504:507	arg1	solids					509:514	high solids loading (30%)	504:528	high solids loading (30%)	504:528	Results indicated that optimal conditions (119 °C, 142 min) using high solids loading (30%) were achieved at lower temperatures and reaction times than those previously reported in the literature.					
30579104	1	35	theme	Agave	219:223	arg1	pretreatment					249:260	Agave tequilana bagasse (ATB) pretreatment	219:260	Agave tequilana bagasse (ATB) pretreatment	219:260	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	0	36	theme	sugar	37:41	arg1	yields					43:48	high sugar yields	32:48	high sugar yields	32:48	Mild reaction conditions induce high sugar yields during the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate.					
30579104	3	37	theme	reaction	570:577	arg1	times					579:583	reaction times	570:583	reaction times	570:583	Results indicated that optimal conditions (119 °C, 142 min) using high solids loading (30%) were achieved at lower temperatures and reaction times than those previously reported in the literature.					
30579104	5	38	theme	lower	837:841	arg1	content					850:856	lower lignin content	837:856	lower lignin content	837:856	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	1	39	theme	tequilana	225:233	arg1	pretreatment					249:260	Agave tequilana bagasse (ATB) pretreatment	219:260	Agave tequilana bagasse (ATB) pretreatment	219:260	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	0	40	theme	high	32:35	arg1	yields					43:48	high sugar yields	32:48	high sugar yields	32:48	Mild reaction conditions induce high sugar yields during the pretreatment of Agave tequilana bagasse with 1-ethyl-3-methylimidazolium acetate.					
30579104	1	41	theme	bagasse	235:241	arg1	pretreatment					249:260	Agave tequilana bagasse (ATB) pretreatment	219:260	Agave tequilana bagasse (ATB) pretreatment	219:260	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	5	42	theme	enzymatic	745:753	arg1	digestibility					755:767	enzymatic digestibility	745:767	enzymatic digestibility of pretreated ATB	745:785	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	5	43	theme	lignin	843:848	arg1	content					850:856	lower lignin content	837:856	lower lignin content	837:856	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	5	44	from	decrease	807:814	arg1	crystallinity					819:831	crystallinity	819:831	crystallinity	819:831	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	5	44	from	decrease	807:814	arg1	content					850:856	lower lignin content	837:856	lower lignin content	837:856	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	4	45	with	pretreatment	682:693	arg1	[Emim					700:704	[Emim][OAc	700:709	[Emim][OAc	700:709	It was also revealed that solid recovery after pretreatment with [Emim][OAc] is a key factor.					
30579104	5	46	theme	microscopy	876:885	arg1	techniques					887:896	microscopy techniques	876:896	microscopy techniques	876:896	The increase in enzymatic digestibility of pretreated ATB was correlated to a decrease in crystallinity and lower lignin content as observed using microscopy techniques and weaken chemical bonds by Fourier transform infrared spectroscopy.					
30579104	1	47	theme	Sequential	143:152	arg1	designs					189:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	1	48	used	used	202:205	arg2	designs					189:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	1	49	theme	2k	154:155	arg1	designs					189:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	1	50	theme	ATB	244:246	arg1	pretreatment					249:260	Agave tequilana bagasse (ATB) pretreatment	219:260	Agave tequilana bagasse (ATB) pretreatment	219:260	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	3	51	theme	optimal	461:467	arg1	conditions					469:478	optimal conditions	461:478	optimal conditions (119 °C, 142 min) using high solids loading (30%)	461:528	Results indicated that optimal conditions (119 °C, 142 min) using high solids loading (30%) were achieved at lower temperatures and reaction times than those previously reported in the literature.					
30579104	3	52	dep	conditions	469:478	arg1	142 min					489:495	142 min	489:495	142 min	489:495	Results indicated that optimal conditions (119 °C, 142 min) using high solids loading (30%) were achieved at lower temperatures and reaction times than those previously reported in the literature.					
30579104	3	52	dep	conditions	469:478	arg1	119 °C					481:486	119 °C	481:486	119 °C	481:486	Results indicated that optimal conditions (119 °C, 142 min) using high solids loading (30%) were achieved at lower temperatures and reaction times than those previously reported in the literature.					
30579104	1	53	theme	factorial	157:165	arg1	designs					189:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs	143:195	Sequential 2k factorial and central composite designs were used to optimize Agave tequilana bagasse (ATB) pretreatment by using 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]).					
30579104	6	54	theme	xylose	990:995	arg1	Yields					968:973	Yields	968:973	Yields of glucose and xylose in the hydrolysate	968:1014	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30579104	6	55	theme	ATB	1063:1065	arg1	100 kg					1043:1048	100 kg	1043:1048	100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively	1043:1152	Yields of glucose and xylose in the hydrolysate were 41.3, and 13.0 kg per 100 kg of untreated ATB, which are equivalent to glucan and xylan conversions of 75.9% and 82.9%, respectively.					
30448494	2	0	theme	antimicrobial	490:502	arg1	properties					516:525	mechanical, antimicrobial and thermal properties	478:525	mechanical, antimicrobial and thermal properties	478:525	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	2	0	theme	antimicrobial	490:502	arg1	behavior					468:475	the swelling and degradation behavior	439:475	behavior	468:475	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	3	1	from	films	674:678	arg1	release					586:592	The release	582:592	The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films	582:678	The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films was studied.					
30448494	4	2	theme	swelling	718:725	arg1	ratio					727:731	swelling ratio	718:731	swelling ratio	718:731	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	9	3	contain	containing	1248:1257	arg1	films					1242:1246	films	1242:1246	films containing TH	1242:1260	It was found that films containing TH showed slightly higher antimicrobial activity against Staphylococcus aureus than Escherichia coli.					
30448494	9	3	contain	containing	1248:1257	arg2	TH					1259:1260	TH	1259:1260	TH	1259:1260	It was found that films containing TH showed slightly higher antimicrobial activity against Staphylococcus aureus than Escherichia coli.					
30448494	4	4	theme	alginate	790:797	arg1	HA					803:804	HA	803:804	HA	803:804	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	4	4	theme	alginate	790:797	arg1	alginate					790:797	alginate	790:797	alginate	790:797	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	4	4	theme	alginate	790:797	arg1	amounts					779:785	the amounts	775:785	the amounts of alginate and HA	775:804	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	2	5	theme	mechanical	478:487	arg1	properties					516:525	mechanical, antimicrobial and thermal properties	478:525	mechanical, antimicrobial and thermal properties	478:525	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	2	5	theme	mechanical	478:487	arg1	behavior					468:475	the swelling and degradation behavior	439:475	behavior	468:475	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	8	6	theme	hydroxyapatite	1198:1211	arg1	hydroxyapatite					1198:1211	hydroxyapatite	1198:1211	hydroxyapatite	1198:1211	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	8	6	theme	hydroxyapatite	1198:1211	arg1	alginate					1185:1192	alginate	1185:1192	alginate	1185:1192	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	8	6	theme	hydroxyapatite	1198:1211	arg1	amounts					1174:1180	the amounts	1170:1180	the amounts of alginate and hydroxyapatite	1170:1211	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	1	7	with	crosslinking	284:295	arg1	chloride					310:317	calcium chloride	302:317	calcium chloride	302:317	In this study, hydroxyapatite (HA)-containing alginate-gelatin films were prepared by solution casting method by blending alginate (A) and gelatin (G) solutions, followed by crosslinking with calcium chloride.					
30448494	4	8	theme	HA	803:804	arg1	HA					803:804	HA	803:804	HA	803:804	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	4	8	theme	HA	803:804	arg1	alginate					790:797	alginate	790:797	alginate	790:797	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	4	8	theme	HA	803:804	arg1	amounts					779:785	the amounts	775:785	the amounts of alginate and HA	775:804	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	5	9	theme	Scanning	817:824	arg1	microscopy					835:844	Scanning electron microscopy	817:844	Scanning electron microscopy analysis	817:853	Scanning electron microscopy analysis indicated that as the amount of HA in the films increased, the film surface becomes rougher.					
30448494	3	10	theme	tetracycline	597:608	arg1	hydrochloride					610:622	tetracycline hydrochloride	597:622	tetracycline hydrochloride (TH)	597:627	The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films was studied.					
30448494	3	10	theme	tetracycline	597:608	arg1	TH					625:626	TH	625:626	TH	625:626	The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films was studied.					
30448494	4	11	theme	weight	737:742	arg1	loss					744:747	weight loss	737:747	weight loss	737:747	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	5	12	theme	electron	826:833	arg1	microscopy					835:844	Scanning electron microscopy	817:844	Scanning electron microscopy analysis	817:853	Scanning electron microscopy analysis indicated that as the amount of HA in the films increased, the film surface becomes rougher.					
30448494	3	13	theme	hydrochloride	610:622	arg1	release					586:592	The release	582:592	The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films	582:678	The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films was studied.					
30448494	2	14	theme	thermal	508:514	arg1	properties					516:525	mechanical, antimicrobial and thermal properties	478:525	mechanical, antimicrobial and thermal properties	478:525	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	2	14	theme	thermal	508:514	arg1	behavior					468:475	the swelling and degradation behavior	439:475	behavior	468:475	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	1	15	theme	solution	196:203	arg1	method					213:218	solution casting method	196:218	solution casting method	196:218	In this study, hydroxyapatite (HA)-containing alginate-gelatin films were prepared by solution casting method by blending alginate (A) and gelatin (G) solutions, followed by crosslinking with calcium chloride.					
30448494	2	16	theme	film	359:362	arg1	solutions					364:372	film solutions	359:372	film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties	359:525	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	8	17	theme	TH	1116:1117	arg1	TH					1116:1117	TH	1116:1117	TH	1116:1117	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	8	17	theme	TH	1116:1117	arg1	amount					1106:1111	The amount	1102:1111	The amount of TH released from the films within 15 min	1102:1155	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	2	18	theme	films	561:565	arg1	morphologies					532:543	morphologies	532:543	morphologies of the obtained films	532:565	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	5	19	from	amount	877:882	arg1	films					897:901	the films	893:901	the films	893:901	Scanning electron microscopy analysis indicated that as the amount of HA in the films increased, the film surface becomes rougher.					
30448494	1	20	theme	casting	205:211	arg1	method					213:218	solution casting method	196:218	solution casting method	196:218	In this study, hydroxyapatite (HA)-containing alginate-gelatin films were prepared by solution casting method by blending alginate (A) and gelatin (G) solutions, followed by crosslinking with calcium chloride.					
30448494	0	21	theme	alginate-gelatin	46:61	arg1	films					73:77	hydroxyapatite-containing alginate-gelatin composite films	20:77	hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing	20:107	Characterization of hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing.					
30448494	2	22	theme	obtained	552:559	arg1	films					561:565	the obtained films	548:565	the obtained films	548:565	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	6	23	theme	films	981:985	arg1	properties					963:972	The mechanical properties	948:972	The mechanical properties of the films	948:985	The mechanical properties of the films were affected by the amount of HA and the A:G ratio.					
30448494	8	24	theme	alginate	1185:1192	arg1	hydroxyapatite					1198:1211	hydroxyapatite	1198:1211	hydroxyapatite	1198:1211	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	8	24	theme	alginate	1185:1192	arg1	alginate					1185:1192	alginate	1185:1192	alginate	1185:1192	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	8	24	theme	alginate	1185:1192	arg1	amounts					1174:1180	the amounts	1170:1180	the amounts of alginate and hydroxyapatite	1170:1211	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	0	25	theme	hydroxyapatite-containing	20:44	arg1	films					73:77	hydroxyapatite-containing alginate-gelatin composite films	20:77	hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing	20:107	Characterization of hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing.					
30448494	7	26	theme	HA	1057:1058	arg1	Incorporation					1040:1052	Incorporation	1040:1052	Incorporation of HA	1040:1058	Incorporation of HA increased the thermal stability of films.					
30448494	0	27	theme	films	73:77	arg1	Characterization					0:15	Characterization	0:15	Characterization of hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing.	0:108	Characterization of hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing.					
30448494	2	28	dep	w/w	341:343	arg1	%					339:339	%	339:339	%	339:339	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	7	29	theme	films	1095:1099	arg1	stability					1082:1090	the thermal stability	1070:1090	the thermal stability of films	1070:1099	Incorporation of HA increased the thermal stability of films.					
30448494	2	30	theme	different	386:394	arg1	ratios					396:401	different ratios	386:401	different ratios (A:G = 40:60, 50:50, and 60:40)	386:433	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	0	31	theme	composite	63:71	arg1	films					73:77	hydroxyapatite-containing alginate-gelatin composite films	20:77	hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing	20:107	Characterization of hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing.					
30448494	2	32	dep	%	339:339	arg1	20					337:338	20	337:338	20	337:338	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	9	33	theme	higher	1278:1283	arg1	activity					1299:1306	slightly higher antimicrobial activity	1269:1306	slightly higher antimicrobial activity against Staphylococcus aureus	1269:1336	It was found that films containing TH showed slightly higher antimicrobial activity against Staphylococcus aureus than Escherichia coli.					
30448494	4	34	dep	ratio	727:731	arg1	the					714:716	the	714:716	the	714:716	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	2	35	dep	A	404:404	arg1	50:50					417:421	50:50	417:421	50:50	417:421	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	2	35	dep	A	404:404	arg1	60:40					428:432	60:40	428:432	60:40	428:432	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	5	36	theme	film	918:921	arg1	surface					923:929	the film surface	914:929	the film surface	914:929	Scanning electron microscopy analysis indicated that as the amount of HA in the films increased, the film surface becomes rougher.					
30448494	6	37	theme	G	1031:1031	arg1	ratio					1033:1037	the A:G ratio	1025:1037	the A:G ratio	1025:1037	The mechanical properties of the films were affected by the amount of HA and the A:G ratio.					
30448494	8	38	attach	released	1119:1126	arg2	TH					1116:1117	TH	1116:1117	TH	1116:1117	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	8	38	attach	released	1119:1126	arg2	amount					1106:1111	The amount	1102:1111	The amount of TH released from the films within 15 min	1102:1155	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	8	38	attach	released	1119:1126	arg1	films					1137:1141	the films	1133:1141	the films	1133:1141	The amount of TH released from the films within 15 min decreased as the amounts of alginate and hydroxyapatite increased.					
30448494	9	39	theme	antimicrobial	1285:1297	arg1	activity					1299:1306	slightly higher antimicrobial activity	1269:1306	slightly higher antimicrobial activity against Staphylococcus aureus	1269:1336	It was found that films containing TH showed slightly higher antimicrobial activity against Staphylococcus aureus than Escherichia coli.					
30448494	1	40	theme	calcium	302:308	arg1	chloride					310:317	calcium chloride	302:317	calcium chloride	302:317	In this study, hydroxyapatite (HA)-containing alginate-gelatin films were prepared by solution casting method by blending alginate (A) and gelatin (G) solutions, followed by crosslinking with calcium chloride.					
30448494	2	41	theme	degradation	456:466	arg1	properties					516:525	mechanical, antimicrobial and thermal properties	478:525	mechanical, antimicrobial and thermal properties	478:525	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	2	41	theme	degradation	456:466	arg1	behavior					468:475	the swelling and degradation behavior	439:475	behavior	468:475	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	5	42	theme	HA	887:888	arg1	HA					887:888	HA	887:888	HA	887:888	Scanning electron microscopy analysis indicated that as the amount of HA in the films increased, the film surface becomes rougher.					
30448494	5	42	theme	HA	887:888	arg1	amount					877:882	the amount	873:882	the amount of HA in the films	873:901	Scanning electron microscopy analysis indicated that as the amount of HA in the films increased, the film surface becomes rougher.					
30448494	2	43	dep	ratios	396:401	arg1	A					404:404	A	404:404	A	404:404	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	3	44	theme	model	644:648	arg1	drug					650:653	a model drug	642:653	a model drug	642:653	The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films was studied.					
30448494	4	45	theme	films	756:760	arg1	ratio					727:731	swelling ratio	718:731	swelling ratio	718:731	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	4	45	theme	films	756:760	arg1	loss					744:747	weight loss	737:747	weight loss	737:747	It was observed that the swelling ratio and weight loss of the films decreased as the amounts of alginate and HA increased.					
30448494	6	46	theme	A	1029:1029	arg1	ratio					1033:1037	the A:G ratio	1025:1037	the A:G ratio	1025:1037	The mechanical properties of the films were affected by the amount of HA and the A:G ratio.					
30448494	5	47	theme	microscopy	835:844	arg1	analysis					846:853	Scanning electron microscopy analysis	817:853	Scanning electron microscopy analysis	817:853	Scanning electron microscopy analysis indicated that as the amount of HA in the films increased, the film surface becomes rougher.					
30448494	6	48	theme	mechanical	952:961	arg1	properties					963:972	The mechanical properties	948:972	The mechanical properties of the films	948:985	The mechanical properties of the films were affected by the amount of HA and the A:G ratio.					
30448494	3	49	theme	prepared	665:672	arg1	films					674:678	the prepared films	661:678	the prepared films	661:678	The release of tetracycline hydrochloride (TH), selected as a model drug, from the prepared films was studied.					
30448494	2	50	theme	swelling	443:450	arg1	properties					516:525	mechanical, antimicrobial and thermal properties	478:525	mechanical, antimicrobial and thermal properties	478:525	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	2	50	theme	swelling	443:450	arg1	behavior					468:475	the swelling and degradation behavior	439:475	behavior	468:475	HA (1, 2, 5, 10, 20% w/w) was added to film solutions prepared at different ratios (A:G = 40:60, 50:50, and 60:40) and the swelling and degradation behavior, mechanical, antimicrobial and thermal properties, and morphologies of the obtained films were examined.					
30448494	1	51	dep	alginate	232:239	arg1	solutions					261:269	solutions	261:269	solutions	261:269	In this study, hydroxyapatite (HA)-containing alginate-gelatin films were prepared by solution casting method by blending alginate (A) and gelatin (G) solutions, followed by crosslinking with calcium chloride.					
30448494	7	52	theme	thermal	1074:1080	arg1	stability					1082:1090	the thermal stability	1070:1090	the thermal stability of films	1070:1099	Incorporation of HA increased the thermal stability of films.					
30448494	0	53	theme	wound	94:98	arg1	dressing					100:107	a potential wound dressing	82:107	a potential wound dressing	82:107	Characterization of hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing.					
30448494	1	54	theme	-containing	144:154	arg1	films					173:177	hydroxyapatite (HA)-containing alginate-gelatin films	125:177	hydroxyapatite (HA)-containing alginate-gelatin films	125:177	In this study, hydroxyapatite (HA)-containing alginate-gelatin films were prepared by solution casting method by blending alginate (A) and gelatin (G) solutions, followed by crosslinking with calcium chloride.					
30448494	0	55	theme	potential	84:92	arg1	dressing					100:107	a potential wound dressing	82:107	a potential wound dressing	82:107	Characterization of hydroxyapatite-containing alginate-gelatin composite films as a potential wound dressing.					
30448494	6	56	theme	HA	1018:1019	arg1	ratio					1033:1037	the A:G ratio	1025:1037	the A:G ratio	1025:1037	The mechanical properties of the films were affected by the amount of HA and the A:G ratio.					
30448494	6	56	theme	HA	1018:1019	arg1	HA					1018:1019	HA	1018:1019	HA	1018:1019	The mechanical properties of the films were affected by the amount of HA and the A:G ratio.					
30448494	6	56	theme	HA	1018:1019	arg1	amount					1008:1013	the amount	1004:1013	the amount of HA	1004:1019	The mechanical properties of the films were affected by the amount of HA and the A:G ratio.					
30448494	1	57	theme	alginate-gelatin	156:171	arg1	films					173:177	hydroxyapatite (HA)-containing alginate-gelatin films	125:177	hydroxyapatite (HA)-containing alginate-gelatin films	125:177	In this study, hydroxyapatite (HA)-containing alginate-gelatin films were prepared by solution casting method by blending alginate (A) and gelatin (G) solutions, followed by crosslinking with calcium chloride.					
31219201	17	0	theme	water	2351:2355	arg1	content					2357:2363	a significantly higher total tissue water content	2315:2363	a significantly higher total tissue water content in the kidneys	2315:2378	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	17	0	theme	water	2351:2355	arg1	muscle					2390:2395	skeletal muscle	2381:2395	skeletal muscle	2381:2395	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	17	0	theme	water	2351:2355	arg1	lung					2401:2404	lung	2401:2404	lung	2401:2404	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	11	1	theme	different	1438:1446	arg1	protocols					1454:1462	different fluid protocols	1438:1462	different fluid protocols	1438:1462	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	14	2	theme	water	1907:1911	arg1	content					1913:1919	total tissue water content	1894:1919	total tissue water content	1894:1919	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	19	3	from	hypothermia	2671:2681	arg1	rewarming					2656:2664	ECC rewarming	2652:2664	ECC rewarming from hypothermia	2652:2681	The composition of fluid additions appears to be an important factor during ECC rewarming from hypothermia.					
31219201	8	4	theme	cardiac	1054:1060	arg1	instability					1062:1072	cardiac instability	1054:1072	cardiac instability	1054:1072	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	16	5	theme	blood	2209:2213	arg1	flow					2215:2218	blood flow	2209:2218	blood flow to the brain and kidneys	2209:2243	After rewarming, the HES group had significantly increased global O2 delivery and blood flow to the brain and kidneys compared with the saline group.					
31219201	11	6	theme	flow	1491:1494	arg1	distribution					1496:1507	blood flow distribution	1485:1507	blood flow distribution	1485:1507	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	11	7	theme	fluid	1467:1471	arg1	balance					1473:1479	fluid balance	1467:1479	fluid balance	1467:1479	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	14	8	theme	delivery	1932:1939	arg1	Calculations					1808:1819	Calculations	1808:1819	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption	1808:1955	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	6	9	theme	rewarming	645:653	arg1	model					621:625	our rat model	613:625	our rat model of extracorporeal rewarming from hypothermic cardiac arrest	613:685	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	14	10	theme	plasma	1824:1829	arg1	PV					1839:1840	PV	1839:1840	PV	1839:1840	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	14	10	theme	plasma	1824:1829	arg1	volume					1831:1836	plasma volume	1824:1836	plasma volume (PV)	1824:1841	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	7	11	theme	extracorporeal	886:899	arg1	rewarming					901:909	extracorporeal rewarming	886:909	extracorporeal rewarming from hypothermia	886:926	The composition of fluid additions appears to be important during extracorporeal rewarming from hypothermia.					
31219201	14	12	theme	global	1922:1927	arg1	delivery					1932:1939	global O2 delivery	1922:1939	global O2 delivery	1922:1939	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	14	13	theme	content	1913:1919	arg1	Calculations					1808:1819	Calculations	1808:1819	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption	1808:1955	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	6	14	theme	rat	617:619	arg1	model					621:625	our rat model	613:625	our rat model of extracorporeal rewarming from hypothermic cardiac arrest	613:685	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	0	15	theme	flow	136:139	arg1	distribution					141:152	blood flow distribution	130:152	blood flow distribution	130:152	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	2	16	theme	accidental	225:234	arg1	patients					248:255	accidental hypothermia patients	225:255	accidental hypothermia patients	225:255	Mortality in accidental hypothermia patients rewarmed by extracorporeal circulation remains high.					
31219201	14	17	theme	tissue	1900:1905	arg1	content					1913:1919	total tissue water content	1894:1919	total tissue water content	1894:1919	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	3	18	theme	optimal	331:337	arg1	additions					345:353	optimal fluid additions	331:353	optimal fluid additions	331:353	Knowledge concerning optimal fluid additions for extracorporeal rewarming is lacking, with no apparent consensus.					
31219201	14	19	theme	volume	1862:1867	arg1	Calculations					1808:1819	Calculations	1808:1819	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption	1808:1955	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	18	20	theme	crystalloid	2421:2431	arg1	priming					2433:2439	crystalloid priming	2421:2439	crystalloid priming	2421:2439	Compared with crystalloid priming, the use of an iso-oncotic colloid prime generates less tissue oedema and increases PV, CBV and organ blood flow during ECC rewarming.					
31219201	9	21	theme	ECC	1113:1115	arg1	initiation					1099:1108	initiation	1099:1108	initiation of ECC	1099:1115	Hypothermia, along with initiation of ECC, introduces major changes in fluid homeostasis and blood flow.					
31219201	14	22	theme	blood	1882:1886	arg1	flow					1888:1891	organ blood flow	1876:1891	organ blood flow	1876:1891	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	3	23	theme	extracorporeal	359:372	arg1	rewarming					374:382	extracorporeal rewarming	359:382	extracorporeal rewarming	359:382	Knowledge concerning optimal fluid additions for extracorporeal rewarming is lacking, with no apparent consensus.					
31219201	4	24	theme	different	469:477	arg1	effects					479:485	different effects	469:485	different effects	469:485	Does colloid versus crystalloid priming have different effects on fluid balance and blood flow distribution during extracorporeal rewarming?					
31219201	6	25	theme	hydroxyethyl	688:699	arg1	starch					701:706	hydroxyethyl starch	688:706	hydroxyethyl starch	688:706	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	0	26	theme	hydroxyethyl	67:78	arg1	starch					80:85	hydroxyethyl starch	67:85	hydroxyethyl starch	67:85	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	5	27	theme	main	577:580	arg1	finding					582:588	the main finding	573:588	the main finding	573:588	What is the main finding and its importance?					
31219201	5	27	theme	main	577:580	arg1	What					565:568	What	565:568	What	565:568	What is the main finding and its importance?					
31219201	1	28	theme	NEW	155:157	arg1	question					188:195	the central question	176:195	the central question of this study	176:209	NEW FINDINGS What is the central question of this study?					
31219201	1	28	theme	NEW	155:157	arg1	FINDINGS					159:166	NEW FINDINGS	155:166	NEW FINDINGS What is the central question of this study	155:209	NEW FINDINGS What is the central question of this study?					
31219201	4	29	theme	extracorporeal	539:552	arg1	rewarming					554:562	extracorporeal rewarming	539:562	extracorporeal rewarming	539:562	Does colloid versus crystalloid priming have different effects on fluid balance and blood flow distribution during extracorporeal rewarming?					
31219201	10	30	theme	priming	1332:1338	arg1	fluid					1340:1344	priming fluid	1332:1344	priming fluid	1332:1344	Scientific data to recommend best practice use of ECC for rewarming these patients is lacking, and no current guidelines exist concerning the choice of priming fluid for the extracorporeal circuit.					
31219201	8	31	theme	ABSTRACT	929:936	arg1	treatment					1003:1011	the recommended treatment	987:1011	the recommended treatment for accidental hypothermia patients with cardiac instability	987:1072	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	8	31	theme	ABSTRACT	929:936	arg1	Rewarming					938:946	ABSTRACT Rewarming	929:946	ABSTRACT Rewarming by extracorporeal circulation (ECC)	929:982	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	13	32	theme	hydroxyethyl	1781:1792	arg1	HES					1802:1804	HES	1802:1804	HES	1802:1804	During cooling, rats were equally randomized into two groups: an extracorporeal circuit primed with saline or primed with hydroxyethyl starch (HES).					
31219201	13	32	theme	hydroxyethyl	1781:1792	arg1	starch					1794:1799	hydroxyethyl starch	1781:1799	hydroxyethyl starch (HES)	1781:1805	During cooling, rats were equally randomized into two groups: an extracorporeal circuit primed with saline or primed with hydroxyethyl starch (HES).					
31219201	6	33	theme	hypothermic	660:670	arg1	arrest					680:685	hypothermic cardiac arrest	660:685	hypothermic cardiac arrest	660:685	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	0	34	theme	priming	101:107	arg1	Effects					56:62	Effects	56:62	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.	0:153	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	12	35	theme	hypothermic	1611:1621	arg1	arrest					1631:1636	deep hypothermic cardiac arrest	1606:1636	deep hypothermic cardiac arrest	1606:1636	Sixteen anaesthetized rats were cooled to deep hypothermic cardiac arrest and rewarmed by ECC.					
31219201	0	36	from	Effects	56:62	arg1	distribution					141:152	blood flow distribution	130:152	blood flow distribution	130:152	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	0	36	from	Effects	56:62	arg1	balance					118:124	fluid balance	112:124	fluid balance	112:124	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	11	37	theme	primary	1382:1388	arg1	aim					1390:1392	The primary aim	1378:1392	The primary aim of this study	1378:1406	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	10	38	theme	extracorporeal	1354:1367	arg1	circuit					1369:1375	the extracorporeal circuit	1350:1375	the extracorporeal circuit	1350:1375	Scientific data to recommend best practice use of ECC for rewarming these patients is lacking, and no current guidelines exist concerning the choice of priming fluid for the extracorporeal circuit.					
31219201	0	39	theme	fluid	112:116	arg1	balance					118:124	fluid balance	112:124	fluid balance	112:124	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	10	40	theme	Scientific	1180:1189	arg1	data					1191:1194	Scientific data	1180:1194	Scientific data	1180:1194	Scientific data to recommend best practice use of ECC for rewarming these patients is lacking, and no current guidelines exist concerning the choice of priming fluid for the extracorporeal circuit.					
31219201	0	41	from	hypothermia	43:53	arg1	rewarming					15:23	Extracorporeal rewarming	0:23	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.	0:153	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	18	42	theme	tissue	2497:2502	arg1	oedema					2504:2509	less tissue oedema	2492:2509	less tissue oedema	2492:2509	Compared with crystalloid priming, the use of an iso-oncotic colloid prime generates less tissue oedema and increases PV, CBV and organ blood flow during ECC rewarming.					
31219201	6	43	theme	circulating	751:761	arg1	volume					769:774	circulating blood volume	751:774	circulating blood volume	751:774	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	0	44	theme	Extracorporeal	0:13	arg1	rewarming					15:23	Extracorporeal rewarming	0:23	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.	0:153	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	15	45	theme	mean	2016:2019	arg1	pressure					2030:2037	mean arterial pressure	2016:2037	mean arterial pressure	2016:2037	During and after rewarming, the pump flow rate, mean arterial pressure, PV and CBV were significantly higher in HES-treated compared with saline-treated rats.					
31219201	11	46	theme	hypothermic	1536:1546	arg1	arrest					1556:1561	deep hypothermic cardiac arrest	1531:1561	deep hypothermic cardiac arrest	1531:1561	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	7	47	theme	additions	845:853	arg1	important					869:877	important	869:877	important	869:877	The composition of fluid additions appears to be important during extracorporeal rewarming from hypothermia.					
31219201	7	47	theme	additions	845:853	arg1	composition					824:834	The composition	820:834	The composition of fluid additions	820:853	The composition of fluid additions appears to be important during extracorporeal rewarming from hypothermia.					
31219201	16	48	theme	saline	2263:2268	arg1	group					2270:2274	the saline group	2259:2274	the saline group	2259:2274	After rewarming, the HES group had significantly increased global O2 delivery and blood flow to the brain and kidneys compared with the saline group.					
31219201	13	49	theme	extracorporeal	1724:1737	arg1	circuit					1739:1745	an extracorporeal circuit	1721:1745	two groups: an extracorporeal circuit primed with saline or primed with hydroxyethyl starch (HES)	1709:1805	During cooling, rats were equally randomized into two groups: an extracorporeal circuit primed with saline or primed with hydroxyethyl starch (HES).					
31219201	6	50	theme	tissue	723:728	arg1	oedema					730:735	less tissue oedema	718:735	less tissue oedema	718:735	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	8	51	theme	recommended	991:1001	arg1	treatment					1003:1011	the recommended treatment	987:1011	the recommended treatment for accidental hypothermia patients with cardiac instability	987:1072	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	8	51	theme	recommended	991:1001	arg1	Rewarming					938:946	ABSTRACT Rewarming	929:946	ABSTRACT Rewarming by extracorporeal circulation (ECC)	929:982	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	17	52	from	Rats	2277:2280	arg1	group					2296:2300	the saline group	2285:2300	the saline group	2285:2300	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	15	53	theme	pump	2000:2003	arg1	rate					2010:2013	the pump flow rate	1996:2013	the pump flow rate	1996:2013	During and after rewarming, the pump flow rate, mean arterial pressure, PV and CBV were significantly higher in HES-treated compared with saline-treated rats.					
31219201	15	53	theme	pump	2000:2003	arg1	higher					2070:2075	higher	2070:2075	higher	2070:2075	During and after rewarming, the pump flow rate, mean arterial pressure, PV and CBV were significantly higher in HES-treated compared with saline-treated rats.					
31219201	10	54	theme	ECC	1230:1232	arg1	use					1223:1225	practice use	1214:1225	practice use of ECC for rewarming these patients is lacking	1214:1272	Scientific data to recommend best practice use of ECC for rewarming these patients is lacking, and no current guidelines exist concerning the choice of priming fluid for the extracorporeal circuit.					
31219201	14	55	theme	volume	1831:1836	arg1	Calculations					1808:1819	Calculations	1808:1819	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption	1808:1955	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	6	56	from	model	621:625	arg1	arrest					680:685	hypothermic cardiac arrest	660:685	hypothermic cardiac arrest	660:685	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	8	57	theme	hypothermia	1028:1038	arg1	patients					1040:1047	accidental hypothermia patients	1017:1047	accidental hypothermia patients with cardiac instability	1017:1072	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	17	58	theme	higher	2331:2336	arg1	content					2357:2363	a significantly higher total tissue water content	2315:2363	a significantly higher total tissue water content in the kidneys	2315:2378	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	17	58	theme	higher	2331:2336	arg1	muscle					2390:2395	skeletal muscle	2381:2395	skeletal muscle	2381:2395	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	17	58	theme	higher	2331:2336	arg1	lung					2401:2404	lung	2401:2404	lung	2401:2404	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	11	59	theme	fluid	1448:1452	arg1	protocols					1454:1462	different fluid protocols	1438:1462	different fluid protocols	1438:1462	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	9	60	theme	major	1129:1133	arg1	changes					1135:1141	major changes	1129:1141	major changes in fluid homeostasis and blood flow	1129:1177	Hypothermia, along with initiation of ECC, introduces major changes in fluid homeostasis and blood flow.					
31219201	13	61	dep	circuit	1739:1745	arg1	primed					1747:1752	primed	1747:1752	primed with saline	1747:1764	During cooling, rats were equally randomized into two groups: an extracorporeal circuit primed with saline or primed with hydroxyethyl starch (HES).					
31219201	13	61	dep	circuit	1739:1745	arg1	primed					1769:1774	primed	1769:1774	primed with hydroxyethyl starch (HES)	1769:1805	During cooling, rats were equally randomized into two groups: an extracorporeal circuit primed with saline or primed with hydroxyethyl starch (HES).					
31219201	14	62	theme	consumption	1945:1955	arg1	Calculations					1808:1819	Calculations	1808:1819	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption	1808:1955	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	17	63	theme	tissue	2344:2349	arg1	content					2357:2363	a significantly higher total tissue water content	2315:2363	a significantly higher total tissue water content in the kidneys	2315:2378	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	17	63	theme	tissue	2344:2349	arg1	muscle					2390:2395	skeletal muscle	2381:2395	skeletal muscle	2381:2395	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	17	63	theme	tissue	2344:2349	arg1	lung					2401:2404	lung	2401:2404	lung	2401:2404	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	18	64	theme	ECC	2561:2563	arg1	rewarming					2565:2573	ECC rewarming	2561:2573	ECC rewarming	2561:2573	Compared with crystalloid priming, the use of an iso-oncotic colloid prime generates less tissue oedema and increases PV, CBV and organ blood flow during ECC rewarming.					
31219201	6	65	theme	organ	780:784	arg1	flow					792:795	organ blood flow	780:795	organ blood flow	780:795	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	10	66	theme	current	1282:1288	arg1	guidelines					1290:1299	no current guidelines	1279:1299	no current guidelines	1279:1299	Scientific data to recommend best practice use of ECC for rewarming these patients is lacking, and no current guidelines exist concerning the choice of priming fluid for the extracorporeal circuit.					
31219201	19	67	theme	ECC	2652:2654	arg1	rewarming					2656:2664	ECC rewarming	2652:2664	ECC rewarming from hypothermia	2652:2681	The composition of fluid additions appears to be an important factor during ECC rewarming from hypothermia.					
31219201	13	68	dep	groups	1713:1718	arg1	circuit					1739:1745	an extracorporeal circuit	1721:1745	two groups: an extracorporeal circuit primed with saline or primed with hydroxyethyl starch (HES)	1709:1805	During cooling, rats were equally randomized into two groups: an extracorporeal circuit primed with saline or primed with hydroxyethyl starch (HES).					
31219201	4	69	theme	crystalloid	444:454	arg1	priming					456:462	crystalloid priming	444:462	crystalloid priming	444:462	Does colloid versus crystalloid priming have different effects on fluid balance and blood flow distribution during extracorporeal rewarming?					
31219201	14	70	theme	blood	1856:1860	arg1	CBV					1870:1872	CBV	1870:1872	CBV	1870:1872	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	14	70	theme	blood	1856:1860	arg1	volume					1862:1867	circulating blood volume	1844:1867	circulating blood volume (CBV)	1844:1873	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	11	71	theme	study	1402:1406	arg1	aim					1390:1392	The primary aim	1378:1392	The primary aim of this study	1378:1406	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	1	72	theme	central	180:186	arg1	question					188:195	the central question	176:195	the central question of this study	176:209	NEW FINDINGS What is the central question of this study?					
31219201	1	72	theme	central	180:186	arg1	FINDINGS					159:166	NEW FINDINGS	155:166	NEW FINDINGS What is the central question of this study	155:209	NEW FINDINGS What is the central question of this study?					
31219201	6	73	theme	extracorporeal	630:643	arg1	rewarming					645:653	extracorporeal rewarming	630:653	extracorporeal rewarming from hypothermic cardiac arrest	630:685	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	0	74	theme	blood	130:134	arg1	distribution					141:152	blood flow distribution	130:152	blood flow distribution	130:152	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	4	75	theme	fluid	490:494	arg1	balance					496:502	fluid balance	490:502	fluid balance	490:502	Does colloid versus crystalloid priming have different effects on fluid balance and blood flow distribution during extracorporeal rewarming?					
31219201	2	76	theme	hypothermia	236:246	arg1	patients					248:255	accidental hypothermia patients	225:255	accidental hypothermia patients	225:255	Mortality in accidental hypothermia patients rewarmed by extracorporeal circulation remains high.					
31219201	1	77	theme	study	205:209	arg1	question					188:195	the central question	176:195	the central question of this study	176:209	NEW FINDINGS What is the central question of this study?					
31219201	1	77	theme	study	205:209	arg1	FINDINGS					159:166	NEW FINDINGS	155:166	NEW FINDINGS What is the central question of this study	155:209	NEW FINDINGS What is the central question of this study?					
31219201	16	78	theme	HES	2148:2150	arg1	group					2152:2156	the HES group	2144:2156	the HES group	2144:2156	After rewarming, the HES group had significantly increased global O2 delivery and blood flow to the brain and kidneys compared with the saline group.					
31219201	18	79	theme	organ	2537:2541	arg1	flow					2549:2552	organ blood flow	2537:2552	organ blood flow	2537:2552	Compared with crystalloid priming, the use of an iso-oncotic colloid prime generates less tissue oedema and increases PV, CBV and organ blood flow during ECC rewarming.					
31219201	14	80	theme	total	1894:1898	arg1	content					1913:1919	total tissue water content	1894:1919	total tissue water content	1894:1919	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	3	81	theme	fluid	339:343	arg1	additions					345:353	optimal fluid additions	331:353	optimal fluid additions	331:353	Knowledge concerning optimal fluid additions for extracorporeal rewarming is lacking, with no apparent consensus.					
31219201	17	82	theme	saline	2289:2294	arg1	group					2296:2300	the saline group	2285:2300	the saline group	2285:2300	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	2	83	theme	extracorporeal	269:282	arg1	circulation					284:294	extracorporeal circulation	269:294	extracorporeal circulation	269:294	Mortality in accidental hypothermia patients rewarmed by extracorporeal circulation remains high.					
31219201	14	84	theme	flow	1888:1891	arg1	Calculations					1808:1819	Calculations	1808:1819	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption	1808:1955	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	11	85	theme	cardiac	1548:1554	arg1	arrest					1556:1561	deep hypothermic cardiac arrest	1531:1561	deep hypothermic cardiac arrest	1531:1561	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	0	86	theme	starch	80:85	arg1	Effects					56:62	Effects	56:62	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.	0:153	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	9	87	theme	fluid	1146:1150	arg1	homeostasis					1152:1162	fluid homeostasis	1146:1162	fluid homeostasis	1146:1162	Hypothermia, along with initiation of ECC, introduces major changes in fluid homeostasis and blood flow.					
31219201	14	88	theme	organ	1876:1880	arg1	flow					1888:1891	organ blood flow	1876:1891	organ blood flow	1876:1891	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	6	89	from	arrest	680:685	arg1	model					621:625	our rat model	613:625	our rat model of extracorporeal rewarming from hypothermic cardiac arrest	613:685	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	6	89	from	arrest	680:685	arg1	rewarming					645:653	extracorporeal rewarming	630:653	extracorporeal rewarming from hypothermic cardiac arrest	630:685	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	10	90	theme	fluid	1340:1344	arg1	choice					1322:1327	the choice	1318:1327	the choice of priming fluid for the extracorporeal circuit	1318:1375	Scientific data to recommend best practice use of ECC for rewarming these patients is lacking, and no current guidelines exist concerning the choice of priming fluid for the extracorporeal circuit.					
31219201	0	91	theme	saline	94:99	arg1	priming					101:107	saline priming	94:107	saline priming	94:107	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	12	92	theme	deep	1606:1609	arg1	arrest					1631:1636	deep hypothermic cardiac arrest	1606:1636	deep hypothermic cardiac arrest	1606:1636	Sixteen anaesthetized rats were cooled to deep hypothermic cardiac arrest and rewarmed by ECC.					
31219201	18	93	theme	prime	2476:2480	arg1	use					2446:2448	the use	2442:2448	the use of an iso-oncotic colloid prime	2442:2480	Compared with crystalloid priming, the use of an iso-oncotic colloid prime generates less tissue oedema and increases PV, CBV and organ blood flow during ECC rewarming.					
31219201	6	94	theme	cardiac	672:678	arg1	arrest					680:685	hypothermic cardiac arrest	660:685	hypothermic cardiac arrest	660:685	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	11	95	from	effects	1427:1433	arg1	distribution					1496:1507	blood flow distribution	1485:1507	blood flow distribution	1485:1507	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	11	95	from	effects	1427:1433	arg1	balance					1473:1479	fluid balance	1467:1479	fluid balance	1467:1479	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	12	96	theme	cardiac	1623:1629	arg1	arrest					1631:1636	deep hypothermic cardiac arrest	1606:1636	deep hypothermic cardiac arrest	1606:1636	Sixteen anaesthetized rats were cooled to deep hypothermic cardiac arrest and rewarmed by ECC.					
31219201	4	97	theme	flow	514:517	arg1	distribution					519:530	blood flow distribution	508:530	blood flow distribution	508:530	Does colloid versus crystalloid priming have different effects on fluid balance and blood flow distribution during extracorporeal rewarming?					
31219201	19	98	theme	fluid	2595:2599	arg1	additions					2601:2609	fluid additions	2595:2609	fluid additions	2595:2609	The composition of fluid additions appears to be an important factor during ECC rewarming from hypothermia.					
31219201	19	99	theme	additions	2601:2609	arg1	composition					2580:2590	The composition	2576:2590	The composition of fluid additions	2576:2609	The composition of fluid additions appears to be an important factor during ECC rewarming from hypothermia.					
31219201	19	99	theme	additions	2601:2609	arg1	factor					2638:2643	an important factor	2625:2643	an important factor	2625:2643	The composition of fluid additions appears to be an important factor during ECC rewarming from hypothermia.					
31219201	2	100	from	Mortality	212:220	arg1	patients					248:255	accidental hypothermia patients	225:255	accidental hypothermia patients	225:255	Mortality in accidental hypothermia patients rewarmed by extracorporeal circulation remains high.					
31219201	9	101	from	changes	1135:1141	arg1	homeostasis					1152:1162	fluid homeostasis	1146:1162	fluid homeostasis	1146:1162	Hypothermia, along with initiation of ECC, introduces major changes in fluid homeostasis and blood flow.					
31219201	9	101	from	changes	1135:1141	arg1	flow					1174:1177	blood flow	1168:1177	blood flow	1168:1177	Hypothermia, along with initiation of ECC, introduces major changes in fluid homeostasis and blood flow.					
31219201	6	102	theme	blood	763:767	arg1	volume					769:774	circulating blood volume	751:774	circulating blood volume	751:774	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
31219201	16	103	dep	increased	2176:2184	arg1	compared					2245:2252	compared	2245:2252	compared with the saline group	2245:2274	After rewarming, the HES group had significantly increased global O2 delivery and blood flow to the brain and kidneys compared with the saline group.					
31219201	4	104	theme	blood	508:512	arg1	distribution					519:530	blood flow distribution	508:530	blood flow distribution	508:530	Does colloid versus crystalloid priming have different effects on fluid balance and blood flow distribution during extracorporeal rewarming?					
31219201	9	105	theme	blood	1168:1172	arg1	flow					1174:1177	blood flow	1168:1177	blood flow	1168:1177	Hypothermia, along with initiation of ECC, introduces major changes in fluid homeostasis and blood flow.					
31219201	11	106	theme	deep	1531:1534	arg1	arrest					1556:1561	deep hypothermic cardiac arrest	1531:1561	deep hypothermic cardiac arrest	1531:1561	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	4	107	contain	have	464:467	arg1	colloid					429:435	colloid	429:435	colloid	429:435	Does colloid versus crystalloid priming have different effects on fluid balance and blood flow distribution during extracorporeal rewarming?					
31219201	4	107	contain	have	464:467	arg1	priming					456:462	crystalloid priming	444:462	crystalloid priming	444:462	Does colloid versus crystalloid priming have different effects on fluid balance and blood flow distribution during extracorporeal rewarming?					
31219201	4	107	contain	have	464:467	arg2	effects					479:485	different effects	469:485	different effects	469:485	Does colloid versus crystalloid priming have different effects on fluid balance and blood flow distribution during extracorporeal rewarming?					
31219201	0	108	theme	experimental	30:41	arg1	hypothermia					43:53	experimental hypothermia	30:53	experimental hypothermia	30:53	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	10	109	theme	practice	1214:1221	arg1	use					1223:1225	practice use	1214:1225	practice use of ECC for rewarming these patients is lacking	1214:1272	Scientific data to recommend best practice use of ECC for rewarming these patients is lacking, and no current guidelines exist concerning the choice of priming fluid for the extracorporeal circuit.					
31219201	3	110	theme	apparent	404:411	arg1	consensus					413:421	no apparent consensus	401:421	no apparent consensus	401:421	Knowledge concerning optimal fluid additions for extracorporeal rewarming is lacking, with no apparent consensus.					
31219201	17	111	theme	skeletal	2381:2388	arg1	content					2357:2363	a significantly higher total tissue water content	2315:2363	a significantly higher total tissue water content in the kidneys	2315:2378	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	17	111	theme	skeletal	2381:2388	arg1	muscle					2390:2395	skeletal muscle	2381:2395	skeletal muscle	2381:2395	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	15	112	theme	arterial	2021:2028	arg1	pressure					2030:2037	mean arterial pressure	2016:2037	mean arterial pressure	2016:2037	During and after rewarming, the pump flow rate, mean arterial pressure, PV and CBV were significantly higher in HES-treated compared with saline-treated rats.					
31219201	8	113	theme	extracorporeal	951:964	arg1	ECC					979:981	ECC	979:981	ECC	979:981	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	8	113	theme	extracorporeal	951:964	arg1	circulation					966:976	extracorporeal circulation	951:976	extracorporeal circulation (ECC)	951:982	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	14	114	theme	circulating	1844:1854	arg1	CBV					1870:1872	CBV	1870:1872	CBV	1870:1872	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	14	114	theme	circulating	1844:1854	arg1	volume					1862:1867	circulating blood volume	1844:1867	circulating blood volume (CBV)	1844:1873	Calculations of plasma volume (PV), circulating blood volume (CBV), organ blood flow, total tissue water content, global O2 delivery and consumption were made.					
31219201	12	115	theme	anaesthetized	1572:1584	arg1	rats					1586:1589	Sixteen anaesthetized rats	1564:1589	Sixteen anaesthetized rats	1564:1589	Sixteen anaesthetized rats were cooled to deep hypothermic cardiac arrest and rewarmed by ECC.					
31219201	8	116	with	patients	1040:1047	arg1	instability					1062:1072	cardiac instability	1054:1072	cardiac instability	1054:1072	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	18	117	theme	blood	2543:2547	arg1	flow					2549:2552	organ blood flow	2537:2552	organ blood flow	2537:2552	Compared with crystalloid priming, the use of an iso-oncotic colloid prime generates less tissue oedema and increases PV, CBV and organ blood flow during ECC rewarming.					
31219201	17	118	from	content	2357:2363	arg1	kidneys					2372:2378	the kidneys	2368:2378	the kidneys	2368:2378	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	7	119	theme	fluid	839:843	arg1	additions					845:853	fluid additions	839:853	fluid additions	839:853	The composition of fluid additions appears to be important during extracorporeal rewarming from hypothermia.					
31219201	19	120	theme	important	2628:2636	arg1	composition					2580:2590	The composition	2576:2590	The composition of fluid additions	2576:2609	The composition of fluid additions appears to be an important factor during ECC rewarming from hypothermia.					
31219201	19	120	theme	important	2628:2636	arg1	factor					2638:2643	an important factor	2625:2643	an important factor	2625:2643	The composition of fluid additions appears to be an important factor during ECC rewarming from hypothermia.					
31219201	16	121	theme	global	2186:2191	arg1	delivery					2196:2203	O2 delivery	2193:2203	O2 delivery	2193:2203	After rewarming, the HES group had significantly increased global O2 delivery and blood flow to the brain and kidneys compared with the saline group.					
31219201	15	122	theme	saline-treated	2106:2119	arg1	rats					2121:2124	saline-treated rats	2106:2124	saline-treated rats	2106:2124	During and after rewarming, the pump flow rate, mean arterial pressure, PV and CBV were significantly higher in HES-treated compared with saline-treated rats.					
31219201	15	123	theme	flow	2005:2008	arg1	rate					2010:2013	the pump flow rate	1996:2013	the pump flow rate	1996:2013	During and after rewarming, the pump flow rate, mean arterial pressure, PV and CBV were significantly higher in HES-treated compared with saline-treated rats.					
31219201	15	123	theme	flow	2005:2008	arg1	higher					2070:2075	higher	2070:2075	higher	2070:2075	During and after rewarming, the pump flow rate, mean arterial pressure, PV and CBV were significantly higher in HES-treated compared with saline-treated rats.					
31219201	7	124	from	hypothermia	916:926	arg1	rewarming					901:909	extracorporeal rewarming	886:909	extracorporeal rewarming from hypothermia	886:926	The composition of fluid additions appears to be important during extracorporeal rewarming from hypothermia.					
31219201	11	125	theme	blood	1485:1489	arg1	distribution					1496:1507	blood flow distribution	1485:1507	blood flow distribution	1485:1507	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	0	126	dep	rewarming	15:23	arg1	Effects					56:62	Effects	56:62	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.	0:153	Extracorporeal rewarming from experimental hypothermia: Effects of hydroxyethyl starch versus saline priming on fluid balance and blood flow distribution.					
31219201	8	127	theme	accidental	1017:1026	arg1	patients					1040:1047	accidental hypothermia patients	1017:1047	accidental hypothermia patients with cardiac instability	1017:1072	ABSTRACT Rewarming by extracorporeal circulation (ECC) is the recommended treatment for accidental hypothermia patients with cardiac instability.					
31219201	17	128	theme	total	2338:2342	arg1	content					2357:2363	a significantly higher total tissue water content	2315:2363	a significantly higher total tissue water content in the kidneys	2315:2378	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	17	128	theme	total	2338:2342	arg1	muscle					2390:2395	skeletal muscle	2381:2395	skeletal muscle	2381:2395	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	17	128	theme	total	2338:2342	arg1	lung					2401:2404	lung	2401:2404	lung	2401:2404	Rats in the saline group demonstrated a significantly higher total tissue water content in the kidneys, skeletal muscle and lung.					
31219201	11	129	theme	protocols	1454:1462	arg1	effects					1427:1433	the effects	1423:1433	the effects of different fluid protocols on fluid balance and blood flow distribution	1423:1507	The primary aim of this study was to compare the effects of different fluid protocols on fluid balance and blood flow distribution during rewarming from deep hypothermic cardiac arrest.					
31219201	6	130	theme	blood	786:790	arg1	flow					792:795	organ blood flow	780:795	organ blood flow	780:795	In our rat model of extracorporeal rewarming from hypothermic cardiac arrest, hydroxyethyl starch generates less tissue oedema and increases circulating blood volume and organ blood flow, compared with saline.					
30912251	6	0	theme	hydrolysate	885:895	arg1	composition					852:862	the composition	848:862	the composition of a lignocellulosic hydrolysate	848:895	Growth in medium based on a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate promoted BNC productivity and yield, but decreased fibril diameter, DPv , crystallinity and thermal stability.					
30912251	0	1	theme	culture	84:90	arg1	media					92:96	culture media	84:96	culture media based on seven sugars from biomass	84:131	Comparison of productivity and quality of bacterial nanocellulose synthesized using culture media based on seven sugars from biomass.					
30912251	6	2	theme	lignocellulosic	869:883	arg1	hydrolysate					885:895	a lignocellulosic hydrolysate	867:895	a lignocellulosic hydrolysate	867:895	Growth in medium based on a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate promoted BNC productivity and yield, but decreased fibril diameter, DPv , crystallinity and thermal stability.					
30912251	7	3	theme	sugar	1164:1168	arg1	mixtures					1170:1177	industrially relevant sugar mixtures	1142:1177	industrially relevant sugar mixtures	1142:1177	This work shows that volumetric productivity, yield and properties of BNC are highly affected by the carbon source, and indicates how industrially relevant sugar mixtures would affect these characteristics.					
30912251	7	4	theme	BNC	1078:1080	arg1	properties					1064:1073	properties	1064:1073	properties	1064:1073	This work shows that volumetric productivity, yield and properties of BNC are highly affected by the carbon source, and indicates how industrially relevant sugar mixtures would affect these characteristics.					
30912251	7	4	theme	BNC	1078:1080	arg1	yield					1054:1058	yield	1054:1058	yield	1054:1058	This work shows that volumetric productivity, yield and properties of BNC are highly affected by the carbon source, and indicates how industrially relevant sugar mixtures would affect these characteristics.					
30912251	7	4	theme	BNC	1078:1080	arg1	productivity					1040:1051	volumetric productivity	1029:1051	volumetric productivity	1029:1051	This work shows that volumetric productivity, yield and properties of BNC are highly affected by the carbon source, and indicates how industrially relevant sugar mixtures would affect these characteristics.					
30912251	2	5	theme	sugar	296:300	arg1	mixture					302:308	one sugar mixture	292:308	one sugar mixture	292:308	seven biomass-derived sugars and one sugar mixture.					
30912251	2	6	link	biomass-derived	265:279	arg1	sugars					281:286	seven biomass-derived sugars	259:286	seven biomass-derived sugars	259:286	seven biomass-derived sugars and one sugar mixture.					
30912251	3	7	theme	bacterial	347:355	arg1	nanocellulose					357:369	the bacterial nanocellulose	343:369	the bacterial nanocellulose (BNC) produced in the different media	343:407	The productivity and quality of the bacterial nanocellulose (BNC) produced in the different media were compared.					
30912251	3	7	theme	bacterial	347:355	arg1	BNC					372:374	BNC	372:374	BNC	372:374	The productivity and quality of the bacterial nanocellulose (BNC) produced in the different media were compared.					
30912251	4	8	from	degree	494:499	arg1	sugar					475:479	consumed sugar	466:479	consumed sugar	466:479	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	3	9	theme	nanocellulose	357:369	arg1	quality					332:338	quality	332:338	quality	332:338	The productivity and quality of the bacterial nanocellulose (BNC) produced in the different media were compared.					
30912251	3	9	theme	nanocellulose	357:369	arg1	productivity					315:326	productivity	315:326	productivity	315:326	The productivity and quality of the bacterial nanocellulose (BNC) produced in the different media were compared.					
30912251	7	10	theme	carbon	1109:1114	arg1	source					1116:1121	the carbon source	1105:1121	the carbon source	1105:1121	This work shows that volumetric productivity, yield and properties of BNC are highly affected by the carbon source, and indicates how industrially relevant sugar mixtures would affect these characteristics.					
30912251	6	11	theme	thermal	989:995	arg1	stability					997:1005	thermal stability	989:1005	thermal stability	989:1005	Growth in medium based on a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate promoted BNC productivity and yield, but decreased fibril diameter, DPv , crystallinity and thermal stability.					
30912251	4	12	theme	viscometric	482:492	arg1	degree					494:499	viscometric degree	482:499	viscometric degree of polymerization (DPv , 4350-4400)	482:535	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	6	13	from	Growth	785:790	arg1	medium					795:800	medium	795:800	medium based on a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate	795:895	Growth in medium based on a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate promoted BNC productivity and yield, but decreased fibril diameter, DPv , crystallinity and thermal stability.					
30912251	6	14	theme	fibril	948:953	arg1	diameter					955:962	fibril diameter	948:962	fibril diameter	948:962	Growth in medium based on a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate promoted BNC productivity and yield, but decreased fibril diameter, DPv , crystallinity and thermal stability.					
30912251	4	15	from	stability	549:557	arg1	sugar					475:479	consumed sugar	466:479	consumed sugar	466:479	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	4	16	theme	thermal	541:547	arg1	stability					549:557	thermal stability	541:557	thermal stability	541:557	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	5	17	theme	lower	680:684	arg1	productivity					697:708	lower volumetric productivity	680:708	lower volumetric productivity	680:708	Growth in media based on xylose, mannose or galactose resulted in lower volumetric productivity and DPv , but in larger fibril diameter and higher crystallinity (76-78%).					
30912251	1	18	theme	culture	205:211	arg1	media					213:217	eight culture media	199:217	eight culture media based on different carbon sources, viz	199:256	Komagataeibacter xylinus ATCC 23770 was statically cultivated in eight culture media based on different carbon sources, viz.					
30912251	0	19	theme	productivity	14:25	arg1	Comparison					0:9	Comparison	0:9	Comparison of productivity and quality of bacterial nanocellulose	0:64	Comparison of productivity and quality of bacterial nanocellulose synthesized using culture media based on seven sugars from biomass.					
30912251	5	20	from	Growth	614:619	arg1	media					624:628	media	624:628	media based on xylose, mannose or galactose	624:666	Growth in media based on xylose, mannose or galactose resulted in lower volumetric productivity and DPv , but in larger fibril diameter and higher crystallinity (76-78%).					
30912251	5	21	theme	volumetric	686:695	arg1	productivity					697:708	lower volumetric productivity	680:708	lower volumetric productivity	680:708	Growth in media based on xylose, mannose or galactose resulted in lower volumetric productivity and DPv , but in larger fibril diameter and higher crystallinity (76-78%).					
30912251	5	22	theme	higher	754:759	arg1	crystallinity					761:773	higher crystallinity	754:773	higher crystallinity (76-78%)	754:782	Growth in media based on xylose, mannose or galactose resulted in lower volumetric productivity and DPv , but in larger fibril diameter and higher crystallinity (76-78%).					
30912251	5	22	theme	higher	754:759	arg1	%					781:781	76-78%	776:781	76-78%	776:781	Growth in media based on xylose, mannose or galactose resulted in lower volumetric productivity and DPv , but in larger fibril diameter and higher crystallinity (76-78%).					
30912251	4	23	from	yield	457:461	arg1	sugar					475:479	consumed sugar	466:479	consumed sugar	466:479	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	6	24	theme	sugar	823:827	arg1	mixture					829:835	a synthetic sugar mixture	811:835	a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate	811:895	Growth in medium based on a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate promoted BNC productivity and yield, but decreased fibril diameter, DPv , crystallinity and thermal stability.					
30912251	0	25	theme	quality	31:37	arg1	Comparison					0:9	Comparison	0:9	Comparison of productivity and quality of bacterial nanocellulose	0:64	Comparison of productivity and quality of bacterial nanocellulose synthesized using culture media based on seven sugars from biomass.					
30912251	4	26	theme	4350-4400	526:534	arg1	DPv					520:522	DPv	520:522	DPv	520:522	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	7	27	theme	volumetric	1029:1038	arg1	productivity					1040:1051	volumetric productivity	1029:1051	volumetric productivity	1029:1051	This work shows that volumetric productivity, yield and properties of BNC are highly affected by the carbon source, and indicates how industrially relevant sugar mixtures would affect these characteristics.					
30912251	4	28	theme	consumed	466:473	arg1	sugar					475:479	consumed sugar	466:479	consumed sugar	466:479	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	7	29	theme	relevant	1155:1162	arg1	mixtures					1170:1177	industrially relevant sugar mixtures	1142:1177	industrially relevant sugar mixtures	1142:1177	This work shows that volumetric productivity, yield and properties of BNC are highly affected by the carbon source, and indicates how industrially relevant sugar mixtures would affect these characteristics.					
30912251	3	30	theme	different	393:401	arg1	media					403:407	the different media	389:407	the different media	389:407	The productivity and quality of the bacterial nanocellulose (BNC) produced in the different media were compared.					
30912251	4	31	from	productivity	443:454	arg1	sugar					475:479	consumed sugar	466:479	consumed sugar	466:479	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	5	32	theme	fibril	734:739	arg1	diameter					741:748	larger fibril diameter	727:748	larger fibril diameter	727:748	Growth in media based on xylose, mannose or galactose resulted in lower volumetric productivity and DPv , but in larger fibril diameter and higher crystallinity (76-78%).					
30912251	0	33	theme	bacterial	42:50	arg1	nanocellulose					52:64	bacterial nanocellulose	42:64	bacterial nanocellulose	42:64	Comparison of productivity and quality of bacterial nanocellulose synthesized using culture media based on seven sugars from biomass.					
30912251	4	34	dep	polymerization	504:517	arg1	DPv					520:522	DPv	520:522	DPv	520:522	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	4	35	theme	Highest	424:430	arg1	productivity					443:454	Highest volumetric productivity	424:454	Highest volumetric productivity	424:454	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	6	36	theme	synthetic	813:821	arg1	mixture					829:835	a synthetic sugar mixture	811:835	a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate	811:895	Growth in medium based on a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate promoted BNC productivity and yield, but decreased fibril diameter, DPv , crystallinity and thermal stability.					
30912251	6	37	theme	BNC	906:908	arg1	productivity					910:921	BNC productivity	906:921	BNC productivity	906:921	Growth in medium based on a synthetic sugar mixture resembling the composition of a lignocellulosic hydrolysate promoted BNC productivity and yield, but decreased fibril diameter, DPv , crystallinity and thermal stability.					
30912251	2	38	theme	biomass-derived	265:279	arg1	sugars					281:286	seven biomass-derived sugars	259:286	seven biomass-derived sugars	259:286	seven biomass-derived sugars and one sugar mixture.					
30912251	1	39	theme	different	228:236	arg1	sources					245:251	different carbon sources	228:251	different carbon sources	228:251	Komagataeibacter xylinus ATCC 23770 was statically cultivated in eight culture media based on different carbon sources, viz.					
30912251	1	39	theme	different	228:236	arg1	viz					254:256	viz	254:256	viz	254:256	Komagataeibacter xylinus ATCC 23770 was statically cultivated in eight culture media based on different carbon sources, viz.					
30912251	1	40	theme	Komagataeibacter	134:149	arg1	ATCC					159:162	Komagataeibacter xylinus ATCC 23770	134:168	Komagataeibacter xylinus ATCC 23770	134:168	Komagataeibacter xylinus ATCC 23770 was statically cultivated in eight culture media based on different carbon sources, viz.					
30912251	1	41	theme	carbon	238:243	arg1	sources					245:251	different carbon sources	228:251	different carbon sources	228:251	Komagataeibacter xylinus ATCC 23770 was statically cultivated in eight culture media based on different carbon sources, viz.					
30912251	1	41	theme	carbon	238:243	arg1	viz					254:256	viz	254:256	viz	254:256	Komagataeibacter xylinus ATCC 23770 was statically cultivated in eight culture media based on different carbon sources, viz.					
30912251	5	42	theme	larger	727:732	arg1	diameter					741:748	larger fibril diameter	727:748	larger fibril diameter	727:748	Growth in media based on xylose, mannose or galactose resulted in lower volumetric productivity and DPv , but in larger fibril diameter and higher crystallinity (76-78%).					
30912251	4	43	theme	volumetric	432:441	arg1	productivity					443:454	Highest volumetric productivity	424:454	Highest volumetric productivity	424:454	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	1	44	theme	xylinus	151:157	arg1	ATCC					159:162	Komagataeibacter xylinus ATCC 23770	134:168	Komagataeibacter xylinus ATCC 23770	134:168	Komagataeibacter xylinus ATCC 23770 was statically cultivated in eight culture media based on different carbon sources, viz.					
30912251	0	45	from	biomass	125:131	arg1	sugars					113:118	seven sugars	107:118	seven sugars from biomass	107:131	Comparison of productivity and quality of bacterial nanocellulose synthesized using culture media based on seven sugars from biomass.					
30912251	0	46	theme	nanocellulose	52:64	arg1	productivity					14:25	productivity	14:25	productivity	14:25	Comparison of productivity and quality of bacterial nanocellulose synthesized using culture media based on seven sugars from biomass.					
30912251	0	46	theme	nanocellulose	52:64	arg1	quality					31:37	quality	31:37	quality	31:37	Comparison of productivity and quality of bacterial nanocellulose synthesized using culture media based on seven sugars from biomass.					
30912251	4	47	theme	polymerization	504:517	arg1	yield					457:461	yield	457:461	yield on consumed sugar	457:479	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	4	47	theme	polymerization	504:517	arg1	stability					549:557	thermal stability	541:557	thermal stability	541:557	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	4	47	theme	polymerization	504:517	arg1	productivity					443:454	Highest volumetric productivity	424:454	Highest volumetric productivity	424:454	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
30912251	4	47	theme	polymerization	504:517	arg1	degree					494:499	viscometric degree	482:499	viscometric degree of polymerization (DPv , 4350-4400)	482:535	Highest volumetric productivity, yield on consumed sugar, viscometric degree of polymerization (DPv , 4350-4400) and thermal stability were achieved using media based on glucose or maltose.					
29857109	6	0	used	used	933:936	arg2	films					920:924	PLA/NCS composite films	902:924	PLA/NCS composite films	902:924	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	4	1	theme	Antimicrobial	519:531	arg1	properties					533:542	Antimicrobial properties	519:542	Antimicrobial properties of PLA/NCS films	519:559	Antimicrobial properties of PLA/NCS films have been confirmed against aerobic microorganisms.					
29857109	5	2	theme	PLA/NCS	613:619	arg1	films					636:640	PLA/NCS solvent casted films	613:640	PLA/NCS solvent casted films	613:640	PLA/NCS solvent casted films were used to pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days.					
29857109	3	3	theme	significant	443:453	arg1	effect					455:460	a significant effect	441:460	a significant effect	441:460	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol had a significant effect on the tensile strength and the heat sealing properties.					
29857109	6	4	theme	PLA/NCS	856:862	arg1	film					864:867	different PLA/NCS film	846:867	different PLA/NCS film	846:867	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	5	5	theme	solvent	621:627	arg1	films					636:640	PLA/NCS solvent casted films	613:640	PLA/NCS solvent casted films	613:640	PLA/NCS solvent casted films were used to pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days.					
29857109	3	6	theme	heat	494:497	arg1	properties					507:516	the heat sealing properties	490:516	the heat sealing properties	490:516	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol had a significant effect on the tensile strength and the heat sealing properties.					
29857109	6	7	theme	different	846:854	arg1	film					864:867	different PLA/NCS film	846:867	different PLA/NCS film	846:867	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	5	8	theme	casted	629:634	arg1	films					636:640	PLA/NCS solvent casted films	613:640	PLA/NCS solvent casted films	613:640	PLA/NCS solvent casted films were used to pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days.					
29857109	1	9	from	nanochitosan	137:148	arg1	matrix					195:200	a polylactic acid (PLA) matrix	171:200	a polylactic acid (PLA) matrix	171:200	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	3	10	theme	polyethylene	417:428	arg1	glycol					430:435	polyethylene glycol	417:435	polyethylene glycol	417:435	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol had a significant effect on the tensile strength and the heat sealing properties.					
29857109	5	11	used	used	647:650	arg2	films					636:640	PLA/NCS solvent casted films	613:640	PLA/NCS solvent casted films	613:640	PLA/NCS solvent casted films were used to pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days.					
29857109	3	12	contain	had	437:439	arg1	interaction					350:360	The interaction	346:360	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol	346:435	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol had a significant effect on the tensile strength and the heat sealing properties.					
29857109	3	12	contain	had	437:439	arg2	effect					455:460	a significant effect	441:460	a significant effect	441:460	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol had a significant effect on the tensile strength and the heat sealing properties.					
29857109	3	13	theme	tensile	469:475	arg1	strength					477:484	the tensile strength	465:484	the tensile strength	465:484	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol had a significant effect on the tensile strength and the heat sealing properties.					
29857109	3	14	theme	polylactic	374:383	arg1	PLA/NCS					404:410	PLA/NCS	404:410	PLA/NCS	404:410	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol had a significant effect on the tensile strength and the heat sealing properties.					
29857109	3	14	theme	polylactic	374:383	arg1	acid/nanochitosan					385:401	the polylactic acid/nanochitosan	370:401	the polylactic acid/nanochitosan (PLA/NCS)	370:411	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol had a significant effect on the tensile strength and the heat sealing properties.					
29857109	0	15	theme	acid/chitosan	11:23	arg1	films					25:29	Polylactic acid/chitosan films	0:29	Polylactic acid/chitosan films for packaging of Indian white prawn (Fenneropenaeus indicus).	0:91	Polylactic acid/chitosan films for packaging of Indian white prawn (Fenneropenaeus indicus).					
29857109	6	16	theme	samples	828:834	arg1	indices					811:817	microbial and biochemical quality indices	777:817	microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film	777:867	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	3	17	theme	sealing	499:505	arg1	properties					507:516	the heat sealing properties	490:516	the heat sealing properties	490:516	The interaction between the polylactic acid/nanochitosan (PLA/NCS) and polyethylene glycol had a significant effect on the tensile strength and the heat sealing properties.					
29857109	0	18	theme	Polylactic	0:9	arg1	films					25:29	Polylactic acid/chitosan films	0:29	Polylactic acid/chitosan films for packaging of Indian white prawn (Fenneropenaeus indicus).	0:91	Polylactic acid/chitosan films for packaging of Indian white prawn (Fenneropenaeus indicus).					
29857109	1	19	theme	polyethylene	208:219	arg1	agent					247:251	a cross linking agent	231:251	a cross linking agent	231:251	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	1	19	theme	polyethylene	208:219	arg1	glycol					221:226	polyethylene glycol	208:226	polyethylene glycol	208:226	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	4	20	theme	PLA/NCS	547:553	arg1	films					555:559	PLA/NCS films	547:559	PLA/NCS films	547:559	Antimicrobial properties of PLA/NCS films have been confirmed against aerobic microorganisms.					
29857109	5	21	theme	pack	655:658	arg1	meat					666:669	pack prawn meat	655:669	pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days	655:752	PLA/NCS solvent casted films were used to pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days.					
29857109	1	22	dep	nanochitosan	137:148	arg1	%					158:158	1%	157:158	1%	157:158	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	1	22	dep	nanochitosan	137:148	arg1	%					165:165	2%	164:165	2%	164:165	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	1	22	dep	nanochitosan	137:148	arg1	%					154:154	0.5%	151:154	0.5%	151:154	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	5	23	theme	prawn	660:664	arg1	meat					666:669	pack prawn meat	655:669	pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days	655:752	PLA/NCS solvent casted films were used to pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days.					
29857109	6	24	theme	prawn	822:826	arg1	samples					828:834	prawn samples	822:834	prawn samples packed in different PLA/NCS film	822:867	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	0	25	dep	prawn	61:65	arg1	indicus					83:89	Fenneropenaeus indicus	68:89	Fenneropenaeus indicus	68:89	Polylactic acid/chitosan films for packaging of Indian white prawn (Fenneropenaeus indicus).					
29857109	5	26	theme	chilled	724:730	arg1	condition					732:740	chilled condition	724:740	chilled condition for 18 days	724:752	PLA/NCS solvent casted films were used to pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days.					
29857109	6	27	theme	composite	910:918	arg1	films					920:924	PLA/NCS composite films	902:924	PLA/NCS composite films	902:924	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	6	28	theme	shelf	981:985	arg1	life					987:990	its shelf life	977:990	its shelf life	977:990	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	1	29	theme	cross	233:237	arg1	agent					247:251	a cross linking agent	231:251	a cross linking agent	231:251	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	1	29	theme	cross	233:237	arg1	glycol					221:226	polyethylene glycol	208:226	polyethylene glycol	208:226	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	6	30	theme	prawn	959:963	arg1	packing					942:948	packing	942:948	packing of fresh prawn	942:963	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	2	31	theme	solvent	329:335	arg1	casting					337:343	solvent casting	329:343	solvent casting	329:343	The mixture was casted into films via solvent casting.					
29857109	0	32	theme	white	55:59	arg1	prawn					61:65	Indian white prawn	48:65	Indian white prawn (Fenneropenaeus indicus)	48:90	Polylactic acid/chitosan films for packaging of Indian white prawn (Fenneropenaeus indicus).					
29857109	1	33	theme	linking	239:245	arg1	agent					247:251	a cross linking agent	231:251	a cross linking agent	231:251	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	1	33	theme	linking	239:245	arg1	glycol					221:226	polyethylene glycol	208:226	polyethylene glycol	208:226	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	6	34	theme	quality	803:809	arg1	indices					811:817	microbial and biochemical quality indices	777:817	microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film	777:867	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	0	35	theme	Indian	48:53	arg1	prawn					61:65	Indian white prawn	48:65	Indian white prawn (Fenneropenaeus indicus)	48:90	Polylactic acid/chitosan films for packaging of Indian white prawn (Fenneropenaeus indicus).					
29857109	6	36	theme	biochemical	791:801	arg1	indices					811:817	microbial and biochemical quality indices	777:817	microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film	777:867	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	6	37	theme	PLA/NCS	902:908	arg1	films					920:924	PLA/NCS composite films	902:924	PLA/NCS composite films	902:924	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	1	38	theme	Composite	93:101	arg1	films					103:107	Composite films	93:107	Composite films	93:107	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	4	39	theme	films	555:559	arg1	properties					533:542	Antimicrobial properties	519:542	Antimicrobial properties of PLA/NCS films	519:559	Antimicrobial properties of PLA/NCS films have been confirmed against aerobic microorganisms.					
29857109	6	40	theme	microbial	777:785	arg1	indices					811:817	microbial and biochemical quality indices	777:817	microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film	777:867	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
29857109	1	41	theme	embedding	127:135	arg1	nanochitosan					137:148	embedding nanochitosan	127:148	embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix	127:200	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	1	42	theme	polylactic	173:182	arg1	acid					184:187	polylactic acid	173:187	a polylactic acid (PLA) matrix	171:200	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	1	42	theme	polylactic	173:182	arg1	PLA					190:192	PLA	190:192	PLA	190:192	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	5	43	dep	meat	666:669	arg1	indicus					687:693	Fenneropeneaus indicus	672:693	Fenneropeneaus indicus	672:693	PLA/NCS solvent casted films were used to pack prawn meat (Fenneropeneaus indicus) which was further stored in chilled condition for 18 days.					
29857109	4	44	theme	aerobic	589:595	arg1	microorganisms					597:610	aerobic microorganisms	589:610	aerobic microorganisms	589:610	Antimicrobial properties of PLA/NCS films have been confirmed against aerobic microorganisms.					
29857109	1	45	theme	acid	184:187	arg1	matrix					195:200	a polylactic acid (PLA) matrix	171:200	a polylactic acid (PLA) matrix	171:200	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	0	46	theme	prawn	61:65	arg1	packaging					35:43	packaging	35:43	packaging of Indian white prawn (Fenneropenaeus indicus)	35:90	Polylactic acid/chitosan films for packaging of Indian white prawn (Fenneropenaeus indicus).					
29857109	1	47	theme	polyvinyl	257:265	arg1	alcohol					267:273	polyvinyl alcohol	257:273	polyvinyl alcohol	257:273	Composite films were developed by embedding nanochitosan (0.5%, 1% and 2%) in a polylactic acid (PLA) matrix using polyethylene glycol as a cross linking agent and polyvinyl alcohol as plasticizer.					
29857109	6	48	theme	fresh	953:957	arg1	prawn					959:963	fresh prawn	953:963	fresh prawn	953:963	The study proved that microbial and biochemical quality indices of prawn samples packed in different PLA/NCS film were retained and concluded that PLA/NCS composite films can be used for packing of fresh prawn to increase its shelf life.					
31677578	5	0	theme	high	738:741	arg1	capacity					754:761	the high adsorption capacity	734:761	the high adsorption capacity of GO-COOH/CS spheres (78 mg/g)	734:793	Therefore, the high adsorption capacity of GO-COOH/CS spheres (78 mg/g) was largely retained for the soil Cu2+ fixation.					
31677578	10	1	theme	graphene	1544:1551	arg1	adsorbents					1553:1562	graphene adsorbents	1544:1562	graphene adsorbents	1544:1562	The implications to the design of graphene adsorbents for soil remediation are discussed.					
31677578	6	2	theme	91.4 mg/L	1028:1036	arg1	0.096 mg/g/μM					1005:1017	0.096 mg/g/μM	1005:1017	0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g	1005:1054	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	3	3	from	water	528:532	arg1	immobilization					500:513	the immobilization	496:513	the immobilization of Cu2+ from water and soil	496:541	In this study, we prepared carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil.					
31677578	6	4	theme	qe	958:959	arg1	Ce					938:939	Ce	938:939	Ce of 0.48 mg/L and qe of 31 mg/g	938:970	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	5	5	theme	adsorption	743:752	arg1	capacity					754:761	the high adsorption capacity	734:761	the high adsorption capacity of GO-COOH/CS spheres (78 mg/g)	734:793	Therefore, the high adsorption capacity of GO-COOH/CS spheres (78 mg/g) was largely retained for the soil Cu2+ fixation.					
31677578	7	6	theme	initial	1060:1066	arg1	concentrations					1073:1086	initial Cu2+ concentrations	1060:1086	initial Cu2+ concentrations of 120 mg/L and lower	1060:1108	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	0	7	theme	wheat	103:107	arg1	plants					109:114	wheat plants	103:114	wheat plants	103:114	Carboxylated graphene oxide-chitosan spheres immobilize Cu2+ in soil and reduce its bioaccumulation in wheat plants.					
31677578	1	8	with	interaction	135:145	arg1	pollutants					152:161	pollutants	152:161	pollutants	152:161	Due to the strong interaction with pollutants and the huge adsorption capacity, graphene adsorbents are widely applied in water decontamination.					
31677578	9	9	theme	toxicity	1373:1380	arg1	evaluations					1382:1392	The toxicity evaluations	1369:1392	The toxicity evaluations	1369:1392	The toxicity evaluations suggested that GO-COOH/CS spheres were of low toxicity to wheat seedlings and did not amplify the toxicity of Cu2+.					
31677578	3	10	from	soil	538:541	arg1	immobilization					500:513	the immobilization	496:513	the immobilization of Cu2+ from water and soil	496:541	In this study, we prepared carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil.					
31677578	4	11	theme	internal	580:587	arg1	diffusion					589:597	the internal diffusion	576:597	the internal diffusion of Cu2+ solution	576:614	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	7	12	theme	free	1184:1187	arg1	lower					1227:1231	lower	1227:1231	lower	1227:1231	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	7	12	theme	free	1184:1187	arg1	concentrations					1194:1207	the corresponding free Cu2+ concentrations	1166:1207	the corresponding free Cu2+ concentrations in leachates	1166:1220	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	11	13	theme	high-performance	1680:1695	arg1	adsorbents					1697:1706	high-performance adsorbents	1680:1706	high-performance adsorbents for the immobilization of Cu2+ to reduce Cu2+ bioaccumulation in plants	1680:1778	Overall, our results collectively indicated that porous GO-COOH/CS spheres were high-performance adsorbents for the immobilization of Cu2+ to reduce Cu2+ bioaccumulation in plants.					
31677578	6	14	from	Ce	1022:1023	arg1	0.096 mg/g/μM					1005:1017	0.096 mg/g/μM	1005:1017	0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g	1005:1054	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	8	15	theme	Cu2+	1252:1255	arg1	fixation					1257:1264	The Cu2+ fixation	1248:1264	The Cu2+ fixation on GO-COOH/CS spheres	1248:1286	The Cu2+ fixation on GO-COOH/CS spheres largely reduced its bioaccumulation in wheat roots from 127.8 μg/g to 51.2 μg/g.					
31677578	5	16	theme	soil	824:827	arg1	fixation					834:841	the soil Cu2+ fixation	820:841	the soil Cu2+ fixation	820:841	Therefore, the high adsorption capacity of GO-COOH/CS spheres (78 mg/g) was largely retained for the soil Cu2+ fixation.					
31677578	4	17	theme	adsorptive	686:695	arg1	sites					697:701	the adsorptive sites	682:701	the adsorptive sites on graphene sheets	682:720	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	4	18	from	soil	673:676	arg1	sheets					715:720	graphene sheets	706:720	graphene sheets	706:720	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	2	19	theme	soil	390:393	arg1	components					395:404	soil components	390:404	soil components	390:404	However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components.					
31677578	7	20	theme	fixation	1115:1122	arg1	efficiencies					1124:1135	the fixation efficiencies	1111:1135	the fixation efficiencies	1111:1135	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	7	20	theme	fixation	1115:1122	arg1	higher					1146:1151	higher	1146:1151	higher	1146:1151	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	0	21	from	Cu2+	56:59	arg1	soil					64:67	soil	64:67	soil	64:67	Carboxylated graphene oxide-chitosan spheres immobilize Cu2+ in soil and reduce its bioaccumulation in wheat plants.					
31677578	11	22	theme	Cu2+	1734:1737	arg1	immobilization					1716:1729	the immobilization	1712:1729	the immobilization of Cu2+ to reduce Cu2+ bioaccumulation in plants	1712:1778	Overall, our results collectively indicated that porous GO-COOH/CS spheres were high-performance adsorbents for the immobilization of Cu2+ to reduce Cu2+ bioaccumulation in plants.					
31677578	9	23	theme	wheat	1452:1456	arg1	seedlings					1458:1466	wheat seedlings	1452:1466	wheat seedlings	1452:1466	The toxicity evaluations suggested that GO-COOH/CS spheres were of low toxicity to wheat seedlings and did not amplify the toxicity of Cu2+.					
31677578	2	24	theme	graphene	271:278	arg1	adsorbents					280:289	graphene adsorbents	271:289	graphene adsorbents	271:289	However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components.					
31677578	1	25	theme	huge	171:174	arg1	capacity					187:194	the huge adsorption capacity	167:194	the huge adsorption capacity	167:194	Due to the strong interaction with pollutants and the huge adsorption capacity, graphene adsorbents are widely applied in water decontamination.					
31677578	0	26	theme	graphene	13:20	arg1	spheres					37:43	Carboxylated graphene oxide-chitosan spheres	0:43	Carboxylated graphene oxide-chitosan spheres	0:43	Carboxylated graphene oxide-chitosan spheres immobilize Cu2+ in soil and reduce its bioaccumulation in wheat plants.					
31677578	11	27	theme	Cu2+	1749:1752	arg1	bioaccumulation					1754:1768	Cu2+ bioaccumulation	1749:1768	Cu2+ bioaccumulation in plants	1749:1778	Overall, our results collectively indicated that porous GO-COOH/CS spheres were high-performance adsorbents for the immobilization of Cu2+ to reduce Cu2+ bioaccumulation in plants.					
31677578	11	28	from	bioaccumulation	1754:1768	arg1	plants					1773:1778	plants	1773:1778	plants	1773:1778	Overall, our results collectively indicated that porous GO-COOH/CS spheres were high-performance adsorbents for the immobilization of Cu2+ to reduce Cu2+ bioaccumulation in plants.					
31677578	2	29	from	sites	351:355	arg1	graphene					360:367	graphene	360:367	graphene	360:367	However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components.					
31677578	7	30	theme	Cu2+	1189:1192	arg1	lower					1227:1231	lower	1227:1231	lower	1227:1231	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	7	30	theme	Cu2+	1189:1192	arg1	concentrations					1194:1207	the corresponding free Cu2+ concentrations	1166:1207	the corresponding free Cu2+ concentrations in leachates	1166:1220	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	1	31	theme	adsorption	176:185	arg1	capacity					187:194	the huge adsorption capacity	167:194	the huge adsorption capacity	167:194	Due to the strong interaction with pollutants and the huge adsorption capacity, graphene adsorbents are widely applied in water decontamination.					
31677578	0	32	theme	Carboxylated	0:11	arg1	spheres					37:43	Carboxylated graphene oxide-chitosan spheres	0:43	Carboxylated graphene oxide-chitosan spheres	0:43	Carboxylated graphene oxide-chitosan spheres immobilize Cu2+ in soil and reduce its bioaccumulation in wheat plants.					
31677578	7	33	from	concentrations	1194:1207	arg1	leachates					1212:1220	leachates	1212:1220	leachates	1212:1220	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	2	34	theme	soil	310:313	arg1	remediation					315:325	soil remediation	310:325	soil remediation	310:325	However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components.					
31677578	10	35	theme	soil	1568:1571	arg1	remediation					1573:1583	soil remediation	1568:1583	soil remediation	1568:1583	The implications to the design of graphene adsorbents for soil remediation are discussed.					
31677578	7	36	theme	120 mg/L	1091:1098	arg1	concentrations					1073:1086	initial Cu2+ concentrations	1060:1086	initial Cu2+ concentrations of 120 mg/L and lower	1060:1108	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	4	37	theme	graphene	706:713	arg1	sheets					715:720	graphene sheets	706:720	graphene sheets	706:720	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	3	38	theme	oxide-chitosan	456:469	arg1	spheres					484:490	carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres	434:490	carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil	434:541	In this study, we prepared carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil.					
31677578	0	39	theme	oxide-chitosan	22:35	arg1	spheres					37:43	Carboxylated graphene oxide-chitosan spheres	0:43	Carboxylated graphene oxide-chitosan spheres	0:43	Carboxylated graphene oxide-chitosan spheres immobilize Cu2+ in soil and reduce its bioaccumulation in wheat plants.					
31677578	0	40	from	bioaccumulation	84:98	arg1	plants					109:114	wheat plants	103:114	wheat plants	103:114	Carboxylated graphene oxide-chitosan spheres immobilize Cu2+ in soil and reduce its bioaccumulation in wheat plants.					
31677578	1	41	theme	graphene	197:204	arg1	adsorbents					206:215	graphene adsorbents	197:215	graphene adsorbents	197:215	Due to the strong interaction with pollutants and the huge adsorption capacity, graphene adsorbents are widely applied in water decontamination.					
31677578	9	42	theme	low	1436:1438	arg1	toxicity					1440:1447	low toxicity	1436:1447	low toxicity	1436:1447	The toxicity evaluations suggested that GO-COOH/CS spheres were of low toxicity to wheat seedlings and did not amplify the toxicity of Cu2+.					
31677578	6	43	theme	PC	983:984	arg1	value					986:990	the PC value	979:990	the PC value	979:990	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	8	44	theme	wheat	1327:1331	arg1	roots					1333:1337	wheat roots	1327:1337	wheat roots	1327:1337	The Cu2+ fixation on GO-COOH/CS spheres largely reduced its bioaccumulation in wheat roots from 127.8 μg/g to 51.2 μg/g.					
31677578	8	45	from	127.8 μg/g	1344:1353	arg1	bioaccumulation					1308:1322	its bioaccumulation	1304:1322	its bioaccumulation in wheat roots from 127.8 μg/g	1304:1353	The Cu2+ fixation on GO-COOH/CS spheres largely reduced its bioaccumulation in wheat roots from 127.8 μg/g to 51.2 μg/g.					
31677578	3	46	theme	carboxylated	434:445	arg1	spheres					484:490	carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres	434:490	carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil	434:541	In this study, we prepared carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil.					
31677578	4	47	theme	direct	640:645	arg1	contact					647:653	the direct contact	636:653	the direct contact between the solid soil and the adsorptive sites on graphene sheets	636:720	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	10	48	theme	adsorbents	1553:1562	arg1	design					1534:1539	the design	1530:1539	the design of graphene adsorbents	1530:1562	The implications to the design of graphene adsorbents for soil remediation are discussed.					
31677578	6	49	theme	31 mg/g	964:970	arg1	qe					958:959	qe	958:959	qe	958:959	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	6	49	theme	31 mg/g	964:970	arg1	0.48 mg/L					944:952	0.48 mg/L	944:952	0.48 mg/L	944:952	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	3	50	theme	graphene	447:454	arg1	spheres					484:490	carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres	434:490	carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil	434:541	In this study, we prepared carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil.					
31677578	5	51	theme	GO-COOH/CS	766:775	arg1	78 mg/g					786:792	78 mg/g	786:792	78 mg/g	786:792	Therefore, the high adsorption capacity of GO-COOH/CS spheres (78 mg/g) was largely retained for the soil Cu2+ fixation.					
31677578	5	51	theme	GO-COOH/CS	766:775	arg1	spheres					777:783	GO-COOH/CS spheres	766:783	GO-COOH/CS spheres (78 mg/g)	766:793	Therefore, the high adsorption capacity of GO-COOH/CS spheres (78 mg/g) was largely retained for the soil Cu2+ fixation.					
31677578	6	52	theme	GO-COOH/CS	900:909	arg1	spheres					911:917	GO-COOH/CS spheres	900:917	GO-COOH/CS spheres	900:917	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	6	53	theme	partition	848:856	arg1	coefficient					858:868	The partition coefficient	844:868	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres	844:917	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	6	53	theme	partition	848:856	arg1	PC					871:872	PC	871:872	PC	871:872	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	2	54	theme	adsorptive	340:349	arg1	sites					351:355	the adsorptive sites	336:355	the adsorptive sites on graphene	336:367	However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components.					
31677578	5	55	theme	spheres	777:783	arg1	capacity					754:761	the high adsorption capacity	734:761	the high adsorption capacity of GO-COOH/CS spheres (78 mg/g)	734:793	Therefore, the high adsorption capacity of GO-COOH/CS spheres (78 mg/g) was largely retained for the soil Cu2+ fixation.					
31677578	1	56	with	capacity	187:194	arg1	pollutants					152:161	pollutants	152:161	pollutants	152:161	Due to the strong interaction with pollutants and the huge adsorption capacity, graphene adsorbents are widely applied in water decontamination.					
31677578	8	57	from	fixation	1257:1264	arg1	spheres					1280:1286	GO-COOH/CS spheres	1269:1286	GO-COOH/CS spheres	1269:1286	The Cu2+ fixation on GO-COOH/CS spheres largely reduced its bioaccumulation in wheat roots from 127.8 μg/g to 51.2 μg/g.					
31677578	9	58	theme	GO-COOH/CS	1409:1418	arg1	spheres					1420:1426	GO-COOH/CS spheres	1409:1426	GO-COOH/CS spheres	1409:1426	The toxicity evaluations suggested that GO-COOH/CS spheres were of low toxicity to wheat seedlings and did not amplify the toxicity of Cu2+.					
31677578	9	59	theme	Cu2+	1504:1507	arg1	toxicity					1492:1499	the toxicity	1488:1499	the toxicity of Cu2+	1488:1507	The toxicity evaluations suggested that GO-COOH/CS spheres were of low toxicity to wheat seedlings and did not amplify the toxicity of Cu2+.					
31677578	2	60	used	used	302:305	arg2	adsorbents					280:289	graphene adsorbents	271:289	graphene adsorbents	271:289	However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components.					
31677578	7	61	theme	corresponding	1170:1182	arg1	lower					1227:1231	lower	1227:1231	lower	1227:1231	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	7	61	theme	corresponding	1170:1182	arg1	concentrations					1194:1207	the corresponding free Cu2+ concentrations	1166:1207	the corresponding free Cu2+ concentrations in leachates	1166:1220	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	7	62	theme	lower	1104:1108	arg1	concentrations					1073:1086	initial Cu2+ concentrations	1060:1086	initial Cu2+ concentrations of 120 mg/L and lower	1060:1108	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	7	63	theme	Cu2+	1068:1071	arg1	concentrations					1073:1086	initial Cu2+ concentrations	1060:1086	initial Cu2+ concentrations of 120 mg/L and lower	1060:1108	At initial Cu2+ concentrations of 120 mg/L and lower, the fixation efficiencies were all higher than 99% and the corresponding free Cu2+ concentrations in leachates were lower than 1.0 mg/L.					
31677578	3	64	theme	GO-COOH/CS	472:481	arg1	spheres					484:490	carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres	434:490	carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil	434:541	In this study, we prepared carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil.					
31677578	4	65	from	sites	697:701	arg1	sheets					715:720	graphene sheets	706:720	graphene sheets	706:720	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	6	66	theme	0.48 mg/L	944:952	arg1	Ce					938:939	Ce	938:939	Ce of 0.48 mg/L and qe of 31 mg/g	938:970	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	8	67	theme	GO-COOH/CS	1269:1278	arg1	spheres					1280:1286	GO-COOH/CS spheres	1269:1286	GO-COOH/CS spheres	1269:1286	The Cu2+ fixation on GO-COOH/CS spheres largely reduced its bioaccumulation in wheat roots from 127.8 μg/g to 51.2 μg/g.					
31677578	11	68	theme	porous	1649:1654	arg1	spheres					1667:1673	porous GO-COOH/CS spheres	1649:1673	porous GO-COOH/CS spheres	1649:1673	Overall, our results collectively indicated that porous GO-COOH/CS spheres were high-performance adsorbents for the immobilization of Cu2+ to reduce Cu2+ bioaccumulation in plants.					
31677578	4	69	from	pores	548:552	arg1	GO-COOH/CS					557:566	GO-COOH/CS	557:566	GO-COOH/CS	557:566	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	1	70	theme	strong	128:133	arg1	interaction					135:145	the strong interaction	124:145	the strong interaction with pollutants	124:161	Due to the strong interaction with pollutants and the huge adsorption capacity, graphene adsorbents are widely applied in water decontamination.					
31677578	6	71	theme	78 mg/g	1048:1054	arg1	qe					1042:1043	qe	1042:1043	qe	1042:1043	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	6	71	theme	78 mg/g	1048:1054	arg1	91.4 mg/L					1028:1036	91.4 mg/L	1028:1036	91.4 mg/L	1028:1036	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	11	72	theme	GO-COOH/CS	1656:1665	arg1	spheres					1667:1673	porous GO-COOH/CS spheres	1649:1673	porous GO-COOH/CS spheres	1649:1673	Overall, our results collectively indicated that porous GO-COOH/CS spheres were high-performance adsorbents for the immobilization of Cu2+ to reduce Cu2+ bioaccumulation in plants.					
31677578	2	73	used	occupied	378:385	arg2	graphene					360:367	graphene	360:367	graphene	360:367	However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components.					
31677578	2	73	used	occupied	378:385	arg2	sites					351:355	the adsorptive sites	336:355	the adsorptive sites on graphene	336:367	However, graphene adsorbents are seldom used in soil remediation, because the adsorptive sites on graphene would be occupied by soil components.					
31677578	4	74	theme	solid	667:671	arg1	soil					673:676	the solid soil	663:676	the solid soil	663:676	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	5	75	theme	Cu2+	829:832	arg1	fixation					834:841	the soil Cu2+ fixation	820:841	the soil Cu2+ fixation	820:841	Therefore, the high adsorption capacity of GO-COOH/CS spheres (78 mg/g) was largely retained for the soil Cu2+ fixation.					
31677578	4	76	theme	solution	607:614	arg1	diffusion					589:597	the internal diffusion	576:597	the internal diffusion of Cu2+ solution	576:614	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	6	77	theme	qe	1042:1043	arg1	0.096 mg/g/μM					1005:1017	0.096 mg/g/μM	1005:1017	0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g	1005:1054	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	6	78	theme	Cu2+	879:882	arg1	adsorption					884:893	Cu2+ adsorption	879:893	Cu2+ adsorption onto GO-COOH/CS spheres	879:917	The partition coefficient (PC) for Cu2+ adsorption onto GO-COOH/CS spheres was 4.2 mg/g/μM at Ce of 0.48 mg/L and qe of 31 mg/g, while the PC value decreased to 0.096 mg/g/μM at Ce of 91.4 mg/L and qe of 78 mg/g.					
31677578	4	79	theme	Cu2+	602:605	arg1	solution					607:614	Cu2+ solution	602:614	Cu2+ solution	602:614	The pores in GO-COOH/CS allowed the internal diffusion of Cu2+ solution, while they blocked the direct contact between the solid soil and the adsorptive sites on graphene sheets.					
31677578	3	80	theme	Cu2+	518:521	arg1	immobilization					500:513	the immobilization	496:513	the immobilization of Cu2+ from water and soil	496:541	In this study, we prepared carboxylated graphene oxide-chitosan (GO-COOH/CS) spheres for the immobilization of Cu2+ from water and soil.					
31677578	8	81	from	bioaccumulation	1308:1322	arg1	roots					1333:1337	wheat roots	1327:1337	wheat roots	1327:1337	The Cu2+ fixation on GO-COOH/CS spheres largely reduced its bioaccumulation in wheat roots from 127.8 μg/g to 51.2 μg/g.					
31677578	1	82	theme	water	239:243	arg1	decontamination					245:259	water decontamination	239:259	water decontamination	239:259	Due to the strong interaction with pollutants and the huge adsorption capacity, graphene adsorbents are widely applied in water decontamination.					
30139654	8	0	theme	soluble	1485:1491	arg1	EC					1493:1494	soluble EC	1485:1494	soluble EC	1485:1494	The values of Vmax for immobilized EC and urea were 1.08 μmol min-1 and 1.16 μmol min-1, respectively, whereas they were 0.782 μmol min-1 and 0.931 μmol min-1, respectively, for soluble EC and urea.					
30139654	1	1	theme	rice	160:163	arg1	wine					165:168	rice wine	160:168	rice wine	160:168	Bifunctional acid urease can not only remove urea from rice wine, but also effectively remove ethyl carbamate (EC), thereby reducing harmful substances in rice wine.					
30139654	4	2	theme	oxide/chitosan	572:585	arg1	beads					587:591	graphene oxide/chitosan beads	563:591	graphene oxide/chitosan beads	563:591	The acid urease thus obtained was immobilized on graphene oxide/chitosan beads.					
30139654	0	3	theme	composite	88:96	arg1	beads					98:102	graphene oxide-chitosan composite beads	64:102	graphene oxide-chitosan composite beads	64:102	Characteristics of refold acid urease immobilized covalently by graphene oxide-chitosan composite beads.					
30139654	9	4	used	used	1623:1626	arg2	beads					1612:1616	the beads	1608:1616	the beads	1608:1616	Furthermore, 90% of the original activity of graphene oxide/chitosan-urease beads were retained after the beads were used 10 times, indicating excellent reusability.					
30139654	2	5	theme	Escherichia	334:344	arg1	DE3					356:358	DE3	356:358	DE3	356:358	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	2	5	theme	Escherichia	334:344	arg1	BL21					351:354	Escherichia coli BL21	334:354	Escherichia coli BL21(DE3)	334:359	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	2	6	from	rettgeri	300:307	arg1	body					377:380	an inclusion body	364:380	an inclusion body	364:380	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	2	6	from	rettgeri	300:307	arg1	urease					276:281	Acid urease	271:281	Acid urease from Providencia rettgeri JN-B815	271:315	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	1	7	theme	harmful	238:244	arg1	substances					246:255	harmful substances	238:255	harmful substances	238:255	Bifunctional acid urease can not only remove urea from rice wine, but also effectively remove ethyl carbamate (EC), thereby reducing harmful substances in rice wine.					
30139654	5	8	theme	graphene	645:652	arg1	composite					669:677	the graphene oxide/chitosan composite	641:677	the graphene oxide/chitosan composite	641:677	The recovery of immobilized urease was 77% for the graphene oxide/chitosan composite when coupled for 8 h with 5% glutaraldehyde.					
30139654	2	9	theme	Acid	271:274	arg1	body					377:380	an inclusion body	364:380	an inclusion body	364:380	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	2	9	theme	Acid	271:274	arg1	urease					276:281	Acid urease	271:281	Acid urease from Providencia rettgeri JN-B815	271:315	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	6	10	from	4°C	1081:1083	arg1	days					1014:1017	43 days	1011:1017	43 days for soluble and chitosan/graphene oxide immobilized urease	1011:1076	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	2	11	dep	Escherichia	334:344	arg1	coli					346:349	coli	346:349	coli	346:349	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	3	12	theme	12.3 U mg-1	488:498	arg1	activity					476:483	specific activity	467:483	specific activity of 12.3 U mg-1	467:498	Subsequently, acid urease was refolded gradually using dilution-ultrafiltration and specific activity of 12.3 U mg-1 was obtained.					
30139654	6	13	theme	urease	909:914	arg1	stability					880:888	the stability	876:888	the stability of the immobilized urease	876:914	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	5	14	theme	urease	622:627	arg1	recovery					598:605	The recovery	594:605	The recovery of immobilized urease	594:627	The recovery of immobilized urease was 77% for the graphene oxide/chitosan composite when coupled for 8 h with 5% glutaraldehyde.					
30139654	5	14	theme	urease	622:627	arg1	%					635:635	77%	633:635	77% for the graphene oxide/chitosan composite	633:677	The recovery of immobilized urease was 77% for the graphene oxide/chitosan composite when coupled for 8 h with 5% glutaraldehyde.					
30139654	5	15	theme	immobilized	610:620	arg1	urease					622:627	immobilized urease	610:627	immobilized urease	610:627	The recovery of immobilized urease was 77% for the graphene oxide/chitosan composite when coupled for 8 h with 5% glutaraldehyde.					
30139654	6	16	theme	soluble	1023:1029	arg1	urease					1071:1076	soluble and chitosan/graphene oxide immobilized urease	1023:1076	soluble and chitosan/graphene oxide immobilized urease	1023:1076	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	17	theme	immobilized	897:907	arg1	urease					909:914	the immobilized urease	893:914	the immobilized urease	893:914	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	18	theme	urease	794:799	arg1	activity					801:808	urease activity	794:808	urease activity	794:808	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	19	theme	immobilized	1059:1069	arg1	urease					1071:1076	soluble and chitosan/graphene oxide immobilized urease	1023:1076	soluble and chitosan/graphene oxide immobilized urease	1023:1076	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	7	20	theme	free	1179:1182	arg1	state					1184:1188	the free state	1175:1188	the free state	1175:1188	A significant change in Km occurred during the immobilization; the Km for urea and EC in the free state were 9.14 mM and 386 mM, respectively, whereas they were 5.28 mM and 180 mM, respectively, in the immobilized state.					
30139654	6	21	theme	urease	970:975	arg1	activity					977:984	urease activity	970:984	urease activity	970:984	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	9	22	theme	excellent	1649:1657	arg1	reusability					1659:1669	excellent reusability	1649:1669	excellent reusability	1649:1669	Furthermore, 90% of the original activity of graphene oxide/chitosan-urease beads were retained after the beads were used 10 times, indicating excellent reusability.					
30139654	1	23	theme	rice	260:263	arg1	wine					265:268	rice wine	260:268	rice wine	260:268	Bifunctional acid urease can not only remove urea from rice wine, but also effectively remove ethyl carbamate (EC), thereby reducing harmful substances in rice wine.					
30139654	1	24	theme	Bifunctional	105:116	arg1	urease					123:128	Bifunctional acid urease	105:128	Bifunctional acid urease	105:128	Bifunctional acid urease can not only remove urea from rice wine, but also effectively remove ethyl carbamate (EC), thereby reducing harmful substances in rice wine.					
30139654	3	25	theme	acid	397:400	arg1	urease					402:407	acid urease	397:407	acid urease	397:407	Subsequently, acid urease was refolded gradually using dilution-ultrafiltration and specific activity of 12.3 U mg-1 was obtained.					
30139654	0	26	theme	acid	26:29	arg1	urease					31:36	refold acid urease	19:36	refold acid urease	19:36	Characteristics of refold acid urease immobilized covalently by graphene oxide-chitosan composite beads.					
30139654	6	27	theme	optimum	770:776	arg1	showed					864:869	showed	864:869	showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C	864:1083	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	27	theme	optimum	770:776	arg1	temperature					778:788	the optimum temperature	766:788	the optimum temperature for urease activity	766:808	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	27	theme	optimum	770:776	arg1	Time-dependent					820:833	Time-dependent	820:833	Time-dependent	820:833	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	1	28	theme	acid	118:121	arg1	urease					123:128	Bifunctional acid urease	105:128	Bifunctional acid urease	105:128	Bifunctional acid urease can not only remove urea from rice wine, but also effectively remove ethyl carbamate (EC), thereby reducing harmful substances in rice wine.					
30139654	2	29	theme	Providencia	288:298	arg1	rettgeri					300:307	Providencia rettgeri JN-B815	288:315	Providencia rettgeri JN-B815	288:315	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	6	30	theme	decay	961:965	arg1	half-life					947:955	half-life	947:955	half-life	947:955	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	30	theme	decay	961:965	arg1	12 h					990:993	12 h	990:993	12 h	990:993	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	30	theme	decay	961:965	arg1	t1/2					941:944	the t1/2	937:944	the t1/2 (half-life) of decay in urease activity	937:984	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	31	dep	Time-dependent	820:833	arg1	showed					864:869	showed	864:869	showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C	864:1083	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	31	dep	Time-dependent	820:833	arg1	temperature					778:788	the optimum temperature	766:788	the optimum temperature for urease activity	766:808	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	6	31	dep	Time-dependent	820:833	arg1	Time-dependent					820:833	Time-dependent	820:833	Time-dependent	820:833	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	1	32	theme	ethyl	199:203	arg1	EC					216:217	EC	216:217	EC	216:217	Bifunctional acid urease can not only remove urea from rice wine, but also effectively remove ethyl carbamate (EC), thereby reducing harmful substances in rice wine.					
30139654	1	32	theme	ethyl	199:203	arg1	carbamate					205:213	ethyl carbamate	199:213	ethyl carbamate (EC)	199:218	Bifunctional acid urease can not only remove urea from rice wine, but also effectively remove ethyl carbamate (EC), thereby reducing harmful substances in rice wine.					
30139654	8	33	dep	1.08 μmol min-1	1359:1373	arg1	whereas					1410:1416	whereas	1410:1416	whereas	1410:1416	The values of Vmax for immobilized EC and urea were 1.08 μmol min-1 and 1.16 μmol min-1, respectively, whereas they were 0.782 μmol min-1 and 0.931 μmol min-1, respectively, for soluble EC and urea.					
30139654	5	34	theme	5	705:705	arg1	%					706:706	%	706:706	%	706:706	The recovery of immobilized urease was 77% for the graphene oxide/chitosan composite when coupled for 8 h with 5% glutaraldehyde.					
30139654	8	35	theme	immobilized	1330:1340	arg1	EC					1342:1343	immobilized EC	1330:1343	immobilized EC	1330:1343	The values of Vmax for immobilized EC and urea were 1.08 μmol min-1 and 1.16 μmol min-1, respectively, whereas they were 0.782 μmol min-1 and 0.931 μmol min-1, respectively, for soluble EC and urea.					
30139654	0	36	theme	urease	31:36	arg1	Characteristics					0:14	Characteristics	0:14	Characteristics of refold acid urease	0:36	Characteristics of refold acid urease immobilized covalently by graphene oxide-chitosan composite beads.					
30139654	4	37	theme	acid	518:521	arg1	urease					523:528	The acid urease	514:528	The acid urease thus obtained	514:542	The acid urease thus obtained was immobilized on graphene oxide/chitosan beads.					
30139654	4	38	theme	graphene	563:570	arg1	beads					587:591	graphene oxide/chitosan beads	563:591	graphene oxide/chitosan beads	563:591	The acid urease thus obtained was immobilized on graphene oxide/chitosan beads.					
30139654	9	39	theme	original	1530:1537	arg1	activity					1539:1546	the original activity	1526:1546	the original activity of graphene oxide/chitosan-urease beads	1526:1586	Furthermore, 90% of the original activity of graphene oxide/chitosan-urease beads were retained after the beads were used 10 times, indicating excellent reusability.					
30139654	2	40	theme	inclusion	367:375	arg1	body					377:380	an inclusion body	364:380	an inclusion body	364:380	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	2	40	theme	inclusion	367:375	arg1	urease					276:281	Acid urease	271:281	Acid urease from Providencia rettgeri JN-B815	271:315	Acid urease from Providencia rettgeri JN-B815 was expressed in Escherichia coli BL21(DE3) as an inclusion body.					
30139654	7	41	from	change	1100:1105	arg1	Km					1110:1111	Km	1110:1111	Km	1110:1111	A significant change in Km occurred during the immobilization; the Km for urea and EC in the free state were 9.14 mM and 386 mM, respectively, whereas they were 5.28 mM and 180 mM, respectively, in the immobilized state.					
30139654	9	42	theme	activity	1539:1546	arg1	activity					1539:1546	the original activity	1526:1546	the original activity of graphene oxide/chitosan-urease beads	1526:1586	Furthermore, 90% of the original activity of graphene oxide/chitosan-urease beads were retained after the beads were used 10 times, indicating excellent reusability.					
30139654	9	42	theme	activity	1539:1546	arg1	%					1521:1521	90%	1519:1521	90% of the original activity of graphene oxide/chitosan-urease beads	1519:1586	Furthermore, 90% of the original activity of graphene oxide/chitosan-urease beads were retained after the beads were used 10 times, indicating excellent reusability.					
30139654	7	43	from	5.28 mM	1247:1253	arg1	state					1300:1304	the immobilized state	1284:1304	the immobilized state	1284:1304	A significant change in Km occurred during the immobilization; the Km for urea and EC in the free state were 9.14 mM and 386 mM, respectively, whereas they were 5.28 mM and 180 mM, respectively, in the immobilized state.					
30139654	5	44	theme	%	706:706	arg1	glutaraldehyde					708:721	5% glutaraldehyde	705:721	5% glutaraldehyde	705:721	The recovery of immobilized urease was 77% for the graphene oxide/chitosan composite when coupled for 8 h with 5% glutaraldehyde.					
30139654	6	45	from	t1/2	941:944	arg1	activity					977:984	urease activity	970:984	urease activity	970:984	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	7	46	theme	significant	1088:1098	arg1	change					1100:1105	A significant change	1086:1105	A significant change in Km	1086:1111	A significant change in Km occurred during the immobilization; the Km for urea and EC in the free state were 9.14 mM and 386 mM, respectively, whereas they were 5.28 mM and 180 mM, respectively, in the immobilized state.					
30139654	7	47	theme	immobilized	1288:1298	arg1	state					1300:1304	the immobilized state	1284:1304	the immobilized state	1284:1304	A significant change in Km occurred during the immobilization; the Km for urea and EC in the free state were 9.14 mM and 386 mM, respectively, whereas they were 5.28 mM and 180 mM, respectively, in the immobilized state.					
30139654	9	48	theme	graphene	1551:1558	arg1	beads					1582:1586	graphene oxide/chitosan-urease beads	1551:1586	graphene oxide/chitosan-urease beads	1551:1586	Furthermore, 90% of the original activity of graphene oxide/chitosan-urease beads were retained after the beads were used 10 times, indicating excellent reusability.					
30139654	7	49	from	180 mM	1259:1264	arg1	state					1300:1304	the immobilized state	1284:1304	the immobilized state	1284:1304	A significant change in Km occurred during the immobilization; the Km for urea and EC in the free state were 9.14 mM and 386 mM, respectively, whereas they were 5.28 mM and 180 mM, respectively, in the immobilized state.					
30139654	8	50	theme	Vmax	1321:1324	arg1	values					1311:1316	The values	1307:1316	The values of Vmax for immobilized EC and urea	1307:1352	The values of Vmax for immobilized EC and urea were 1.08 μmol min-1 and 1.16 μmol min-1, respectively, whereas they were 0.782 μmol min-1 and 0.931 μmol min-1, respectively, for soluble EC and urea.					
30139654	8	50	theme	Vmax	1321:1324	arg1	1.08 μmol min-1					1359:1373	1.08 μmol min-1	1359:1373	1.08 μmol min-1	1359:1373	The values of Vmax for immobilized EC and urea were 1.08 μmol min-1 and 1.16 μmol min-1, respectively, whereas they were 0.782 μmol min-1 and 0.931 μmol min-1, respectively, for soluble EC and urea.					
30139654	7	51	from	Km	1153:1154	arg1	state					1184:1188	the free state	1175:1188	the free state	1175:1188	A significant change in Km occurred during the immobilization; the Km for urea and EC in the free state were 9.14 mM and 386 mM, respectively, whereas they were 5.28 mM and 180 mM, respectively, in the immobilized state.					
30139654	6	52	theme	inactivation	843:854	arg1	studies					856:862	thermal inactivation studies	835:862	thermal inactivation studies	835:862	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	9	53	theme	oxide/chitosan-urease	1560:1580	arg1	beads					1582:1586	graphene oxide/chitosan-urease beads	1551:1586	graphene oxide/chitosan-urease beads	1551:1586	Furthermore, 90% of the original activity of graphene oxide/chitosan-urease beads were retained after the beads were used 10 times, indicating excellent reusability.					
30139654	6	54	theme	kinetic	737:743	arg1	analysis					745:752	Steady-state kinetic analysis	724:752	Steady-state kinetic analysis	724:752	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	3	55	theme	specific	467:474	arg1	activity					476:483	specific activity	467:483	specific activity of 12.3 U mg-1	467:498	Subsequently, acid urease was refolded gradually using dilution-ultrafiltration and specific activity of 12.3 U mg-1 was obtained.					
30139654	0	56	theme	oxide-chitosan	73:86	arg1	beads					98:102	graphene oxide-chitosan composite beads	64:102	graphene oxide-chitosan composite beads	64:102	Characteristics of refold acid urease immobilized covalently by graphene oxide-chitosan composite beads.					
30139654	6	57	theme	thermal	835:841	arg1	studies					856:862	thermal inactivation studies	835:862	thermal inactivation studies	835:862	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	9	58	theme	beads	1582:1586	arg1	activity					1539:1546	the original activity	1526:1546	the original activity of graphene oxide/chitosan-urease beads	1526:1586	Furthermore, 90% of the original activity of graphene oxide/chitosan-urease beads were retained after the beads were used 10 times, indicating excellent reusability.					
30139654	6	59	theme	Steady-state	724:735	arg1	analysis					745:752	Steady-state kinetic analysis	724:752	Steady-state kinetic analysis	724:752	Steady-state kinetic analysis showed that the optimum temperature for urease activity was 37°C. Time-dependent thermal inactivation studies showed that the stability of the immobilized urease improved at 37°C and the t1/2 (half-life) of decay in urease activity was 12 h, whereas it was 43 days for soluble and chitosan/graphene oxide immobilized urease at 4°C.					
30139654	5	60	theme	oxide/chitosan	654:667	arg1	composite					669:677	the graphene oxide/chitosan composite	641:677	the graphene oxide/chitosan composite	641:677	The recovery of immobilized urease was 77% for the graphene oxide/chitosan composite when coupled for 8 h with 5% glutaraldehyde.					
30139654	0	61	theme	graphene	64:71	arg1	beads					98:102	graphene oxide-chitosan composite beads	64:102	graphene oxide-chitosan composite beads	64:102	Characteristics of refold acid urease immobilized covalently by graphene oxide-chitosan composite beads.					
30139654	0	62	theme	refold	19:24	arg1	urease					31:36	refold acid urease	19:36	refold acid urease	19:36	Characteristics of refold acid urease immobilized covalently by graphene oxide-chitosan composite beads.					
31051086	0	0	theme	Milk	70:73	arg1	Sulfate					40:46	Chondroitin Sulfate	28:46	Chondroitin Sulfate	28:46	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	0	0	theme	Milk	70:73	arg1	Component					51:59	a Component	49:59	a Component of Human Milk	49:73	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	0	1	from	Influence	15:23	arg1	Translocation					113:125	Translocation	113:125	Translocation	113:125	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	0	1	from	Influence	15:23	arg1	Invasion					100:107	Intestinal Bacterial Invasion	79:107	Intestinal Bacterial Invasion	79:107	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	5	2	theme	cell	909:912	arg1	monolayers					914:923	T84 cell monolayers	905:923	T84 cell monolayers	905:923	METHODS T84 cell monolayers were treated with chondroitin sulfate and cell viability was assessed across a number of doses.					
31051086	3	3	from	intestine	566:574	arg1	prevalent					529:537	prevalent	529:537	prevalent	529:537	Glycosaminoglycans, compounds naturally prevalent in both human milk and the intestine, are thought to be anti-inflammatory and capable of altering bacterial interactions within the gut.					
31051086	0	4	theme	Human	64:68	arg1	Milk					70:73	Human Milk	64:73	Human Milk	64:73	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	4	5	theme	in	881:882	arg1	model					890:894	an intestinal in vitro model	867:894	an intestinal in vitro model	867:894	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	4	6	from	glycosaminoglycans	791:808	arg1	milk					819:822	human milk	813:822	human milk	813:822	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	3	7	theme	human	547:551	arg1	milk					553:556	human milk	547:556	human milk	547:556	Glycosaminoglycans, compounds naturally prevalent in both human milk and the intestine, are thought to be anti-inflammatory and capable of altering bacterial interactions within the gut.					
31051086	10	8	theme	attendant	1789:1797	arg1	reduction					1799:1807	attendant reduction	1789:1807	attendant reduction in cell viability	1789:1825	CONCLUSIONS These data suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability.					
31051086	4	9	from	class	782:786	arg1	milk					819:822	human milk	813:822	human milk	813:822	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	5	10	theme	T84	905:907	arg1	monolayers					914:923	T84 cell monolayers	905:923	T84 cell monolayers	905:923	METHODS T84 cell monolayers were treated with chondroitin sulfate and cell viability was assessed across a number of doses.					
31051086	6	11	theme	compound	1185:1192	arg1	role					1173:1176	any protective role	1158:1176	any protective role of the compound against infection	1158:1210	Monolayers were then pretreated with chondroitin sulfate and subsequently challenged with E. coli invasion and translocation to evaluate any protective role of the compound against infection.					
31051086	3	12	from	milk	553:556	arg1	prevalent					529:537	prevalent	529:537	prevalent	529:537	Glycosaminoglycans, compounds naturally prevalent in both human milk and the intestine, are thought to be anti-inflammatory and capable of altering bacterial interactions within the gut.					
31051086	4	13	theme	prominent	772:780	arg1	class					782:786	the most prominent class	763:786	the most prominent class of glycosaminoglycans in human milk	763:822	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	4	13	theme	prominent	772:780	arg1	sulfate					754:760	chondroitin sulfate	742:760	chondroitin sulfate	742:760	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	1	14	theme	preterm	271:277	arg1	population					279:288	the preterm population	267:288	the preterm population	267:288	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	0	15	theme	Bacterial	90:98	arg1	Invasion					100:107	Intestinal Bacterial Invasion	79:107	Intestinal Bacterial Invasion	79:107	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	6	16	theme	protective	1162:1171	arg1	role					1173:1176	any protective role	1158:1176	any protective role of the compound against infection	1158:1210	Monolayers were then pretreated with chondroitin sulfate and subsequently challenged with E. coli invasion and translocation to evaluate any protective role of the compound against infection.					
31051086	10	17	dep	suggest	1645:1651	arg1	protect					1677:1683	protect	1677:1683	protect	1677:1683	CONCLUSIONS These data suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability.					
31051086	10	18	theme	chondroitin	1653:1663	arg1	sulfate					1665:1671	chondroitin sulfate	1653:1671	chondroitin sulfate	1653:1671	CONCLUSIONS These data suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability.					
31051086	4	19	theme	glycosaminoglycans	791:808	arg1	class					782:786	the most prominent class	763:786	the most prominent class of glycosaminoglycans in human milk	763:822	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	4	19	theme	glycosaminoglycans	791:808	arg1	sulfate					754:760	chondroitin sulfate	742:760	chondroitin sulfate	742:760	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	8	20	dep	μg/ml	1386:1390	arg1	750					1382:1384	750	1382:1384	750	1382:1384	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml was not associated with any statistically significant decrease in cell viability.					
31051086	6	21	theme	E.	1111:1112	arg1	invasion					1119:1126	E. coli invasion	1111:1126	E. coli invasion	1111:1126	Monolayers were then pretreated with chondroitin sulfate and subsequently challenged with E. coli invasion and translocation to evaluate any protective role of the compound against infection.					
31051086	0	22	theme	Intestinal	79:88	arg1	Invasion					100:107	Intestinal Bacterial Invasion	79:107	Intestinal Bacterial Invasion	79:107	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	10	23	theme	bacterial	1693:1701	arg1	infection					1703:1711	bacterial infection	1693:1711	bacterial infection	1693:1711	CONCLUSIONS These data suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability.					
31051086	10	24	from	reduction	1723:1731	arg1	translocation					1754:1766	translocation	1754:1766	translocation	1754:1766	CONCLUSIONS These data suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability.					
31051086	10	24	from	reduction	1723:1731	arg1	invasion					1741:1748	invasion	1741:1748	invasion	1741:1748	CONCLUSIONS These data suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability.					
31051086	2	25	theme	important	472:480	arg1	roles					482:486	important roles	472:486	important roles	472:486	Although the pathogenesis of necrotizing enterocolitis is yet to be solidified, intestinal integrity dysfunction, bacterial invasion and/or translocation, and inflammation may play important roles.					
31051086	8	26	from	decrease	1446:1453	arg1	viability					1463:1471	cell viability	1458:1471	cell viability	1458:1471	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml was not associated with any statistically significant decrease in cell viability.					
31051086	2	27	theme	bacterial	405:413	arg1	invasion					415:422	bacterial invasion	405:422	bacterial invasion	405:422	Although the pathogenesis of necrotizing enterocolitis is yet to be solidified, intestinal integrity dysfunction, bacterial invasion and/or translocation, and inflammation may play important roles.					
31051086	0	28	theme	Protective	4:13	arg1	Influence					15:23	The Protective Influence	0:23	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation	0:125	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	4	29	from	infection	854:862	arg1	model					890:894	an intestinal in vitro model	867:894	an intestinal in vitro model	867:894	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	4	30	theme	intestinal	870:879	arg1	model					890:894	an intestinal in vitro model	867:894	an intestinal in vitro model	867:894	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	9	31	from	decrease	1547:1554	arg1	translocation					1587:1599	translocation	1587:1599	translocation	1587:1599	Additionally, chondroitin sulfate at 750 μg/ml was associated with a 75% decrease in both bacterial invasion and translocation compared to control.					
31051086	9	31	from	decrease	1547:1554	arg1	invasion					1574:1581	bacterial invasion	1564:1581	bacterial invasion	1564:1581	Additionally, chondroitin sulfate at 750 μg/ml was associated with a 75% decrease in both bacterial invasion and translocation compared to control.					
31051086	2	32	theme	enterocolitis	332:344	arg1	pathogenesis					304:315	the pathogenesis	300:315	the pathogenesis of necrotizing enterocolitis	300:344	Although the pathogenesis of necrotizing enterocolitis is yet to be solidified, intestinal integrity dysfunction, bacterial invasion and/or translocation, and inflammation may play important roles.					
31051086	7	33	theme	Tight	1213:1217	arg1	function					1236:1243	Tight junction barrier function	1213:1243	Tight junction barrier function	1213:1243	Tight junction barrier function was assessed by transepithelial electrical resistance, and cytokine levels were evaluated.					
31051086	3	34	from	prevalent	529:537	arg1	milk					553:556	human milk	547:556	human milk	547:556	Glycosaminoglycans, compounds naturally prevalent in both human milk and the intestine, are thought to be anti-inflammatory and capable of altering bacterial interactions within the gut.					
31051086	3	34	from	prevalent	529:537	arg1	intestine					566:574	the intestine	562:574	the intestine	562:574	Glycosaminoglycans, compounds naturally prevalent in both human milk and the intestine, are thought to be anti-inflammatory and capable of altering bacterial interactions within the gut.					
31051086	2	35	theme	necrotizing	320:330	arg1	enterocolitis					332:344	necrotizing enterocolitis	320:344	necrotizing enterocolitis	320:344	Although the pathogenesis of necrotizing enterocolitis is yet to be solidified, intestinal integrity dysfunction, bacterial invasion and/or translocation, and inflammation may play important roles.					
31051086	5	36	theme	doses	1014:1018	arg1	number					1004:1009	a number	1002:1009	a number of doses	1002:1018	METHODS T84 cell monolayers were treated with chondroitin sulfate and cell viability was assessed across a number of doses.					
31051086	5	37	theme	chondroitin	943:953	arg1	sulfate					955:961	chondroitin sulfate	943:961	chondroitin sulfate	943:961	METHODS T84 cell monolayers were treated with chondroitin sulfate and cell viability was assessed across a number of doses.					
31051086	5	38	theme	cell	967:970	arg1	viability					972:980	cell viability	967:980	cell viability	967:980	METHODS T84 cell monolayers were treated with chondroitin sulfate and cell viability was assessed across a number of doses.					
31051086	4	39	theme	sulfate	754:760	arg1	potential					729:737	the potential	725:737	the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model	725:894	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	9	40	theme	%	1545:1545	arg1	decrease					1547:1554	a 75% decrease	1541:1554	a 75% decrease in both bacterial invasion and translocation compared to control	1541:1619	Additionally, chondroitin sulfate at 750 μg/ml was associated with a 75% decrease in both bacterial invasion and translocation compared to control.					
31051086	9	41	from	μg/ml	1515:1519	arg1	sulfate					1500:1506	chondroitin sulfate	1488:1506	chondroitin sulfate at 750 μg/ml	1488:1519	Additionally, chondroitin sulfate at 750 μg/ml was associated with a 75% decrease in both bacterial invasion and translocation compared to control.					
31051086	6	42	dep	E.	1111:1112	arg1	coli					1114:1117	coli	1114:1117	coli	1114:1117	Monolayers were then pretreated with chondroitin sulfate and subsequently challenged with E. coli invasion and translocation to evaluate any protective role of the compound against infection.					
31051086	1	43	theme	necrotizing	184:194	arg1	enterocolitis					196:208	necrotizing enterocolitis	184:208	necrotizing enterocolitis	184:208	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	1	43	theme	necrotizing	184:194	arg1	disease					249:255	a devastating intestinal inflammatory disease	211:255	a devastating intestinal inflammatory disease affecting the preterm population	211:288	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	0	44	theme	Sulfate	40:46	arg1	Influence					15:23	The Protective Influence	0:23	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation	0:125	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	9	45	theme	75	1543:1544	arg1	%					1545:1545	%	1545:1545	%	1545:1545	Additionally, chondroitin sulfate at 750 μg/ml was associated with a 75% decrease in both bacterial invasion and translocation compared to control.					
31051086	8	46	dep	dose	1371:1374	arg1	μg/ml					1386:1390	μg/ml	1386:1390	μg/ml	1386:1390	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml was not associated with any statistically significant decrease in cell viability.					
31051086	6	47	theme	chondroitin	1058:1068	arg1	sulfate					1070:1076	chondroitin sulfate	1058:1076	chondroitin sulfate	1058:1076	Monolayers were then pretreated with chondroitin sulfate and subsequently challenged with E. coli invasion and translocation to evaluate any protective role of the compound against infection.					
31051086	0	48	theme	Chondroitin	28:38	arg1	Sulfate					40:46	Chondroitin Sulfate	28:46	Chondroitin Sulfate	28:46	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	0	48	theme	Chondroitin	28:38	arg1	Component					51:59	a Component	49:59	a Component of Human Milk	49:73	The Protective Influence of Chondroitin Sulfate, a Component of Human Milk, on Intestinal Bacterial Invasion and Translocation.					
31051086	10	49	dep	CONCLUSIONS	1622:1632	arg1	suggest					1645:1651	suggest	1645:1651	suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability	1645:1825	CONCLUSIONS These data suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability.					
31051086	8	50	theme	significant	1434:1444	arg1	decrease					1446:1453	any statistically significant decrease	1416:1453	any statistically significant decrease in cell viability	1416:1471	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml was not associated with any statistically significant decrease in cell viability.					
31051086	4	51	theme	RESEARCH	676:683	arg1	AIM					685:687	RESEARCH AIM	676:687	RESEARCH AIM	676:687	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	8	52	theme	Chondroitin	1344:1354	arg1	sulfate					1356:1362	RESULTS Chondroitin sulfate	1336:1362	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml	1336:1390	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml was not associated with any statistically significant decrease in cell viability.					
31051086	9	53	theme	bacterial	1564:1572	arg1	invasion					1574:1581	bacterial invasion	1564:1581	bacterial invasion	1564:1581	Additionally, chondroitin sulfate at 750 μg/ml was associated with a 75% decrease in both bacterial invasion and translocation compared to control.					
31051086	4	54	theme	bacterial	844:852	arg1	infection					854:862	bacterial infection	844:862	bacterial infection in an intestinal in vitro model	844:894	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	9	55	theme	chondroitin	1488:1498	arg1	sulfate					1500:1506	chondroitin sulfate	1488:1506	chondroitin sulfate at 750 μg/ml	1488:1519	Additionally, chondroitin sulfate at 750 μg/ml was associated with a 75% decrease in both bacterial invasion and translocation compared to control.					
31051086	3	56	theme	prevalent	529:537	arg1	compounds					509:517	compounds	509:517	compounds naturally prevalent in both human milk and the intestine	509:574	Glycosaminoglycans, compounds naturally prevalent in both human milk and the intestine, are thought to be anti-inflammatory and capable of altering bacterial interactions within the gut.					
31051086	3	56	theme	prevalent	529:537	arg1	Glycosaminoglycans					489:506	Glycosaminoglycans	489:506	Glycosaminoglycans	489:506	Glycosaminoglycans, compounds naturally prevalent in both human milk and the intestine, are thought to be anti-inflammatory and capable of altering bacterial interactions within the gut.					
31051086	4	57	dep	in	881:882	arg1	vitro					884:888	vitro	884:888	vitro	884:888	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	8	58	theme	cell	1458:1461	arg1	viability					1463:1471	cell viability	1458:1471	cell viability	1458:1471	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml was not associated with any statistically significant decrease in cell viability.					
31051086	7	59	theme	barrier	1228:1234	arg1	function					1236:1243	Tight junction barrier function	1213:1243	Tight junction barrier function	1213:1243	Tight junction barrier function was assessed by transepithelial electrical resistance, and cytokine levels were evaluated.					
31051086	8	60	from	dose	1371:1374	arg1	sulfate					1356:1362	RESULTS Chondroitin sulfate	1336:1362	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml	1336:1390	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml was not associated with any statistically significant decrease in cell viability.					
31051086	8	61	theme	RESULTS	1336:1342	arg1	sulfate					1356:1362	RESULTS Chondroitin sulfate	1336:1362	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml	1336:1390	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml was not associated with any statistically significant decrease in cell viability.					
31051086	7	62	theme	cytokine	1304:1311	arg1	levels					1313:1318	cytokine levels	1304:1318	cytokine levels	1304:1318	Tight junction barrier function was assessed by transepithelial electrical resistance, and cytokine levels were evaluated.					
31051086	1	63	theme	BACKGROUND	128:137	arg1	milk					145:148	BACKGROUND Human milk	128:148	BACKGROUND Human milk	128:148	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	4	64	theme	human	813:817	arg1	milk					819:822	human milk	813:822	human milk	813:822	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	4	65	theme	chondroitin	742:752	arg1	class					782:786	the most prominent class	763:786	the most prominent class of glycosaminoglycans in human milk	763:822	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	4	65	theme	chondroitin	742:752	arg1	sulfate					754:760	chondroitin sulfate	742:760	chondroitin sulfate	742:760	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	5	66	dep	METHODS	897:903	arg1	treated					930:936	treated	930:936	were treated with chondroitin sulfate and cell viability	925:980	METHODS T84 cell monolayers were treated with chondroitin sulfate and cell viability was assessed across a number of doses.					
31051086	10	67	theme	cell	1812:1815	arg1	viability					1817:1825	cell viability	1812:1825	cell viability	1812:1825	CONCLUSIONS These data suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability.					
31051086	1	68	theme	Human	139:143	arg1	milk					145:148	BACKGROUND Human milk	128:148	BACKGROUND Human milk	128:148	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	1	69	theme	devastating	213:223	arg1	enterocolitis					196:208	necrotizing enterocolitis	184:208	necrotizing enterocolitis	184:208	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	1	69	theme	devastating	213:223	arg1	disease					249:255	a devastating intestinal inflammatory disease	211:255	a devastating intestinal inflammatory disease affecting the preterm population	211:288	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	7	70	theme	transepithelial	1261:1275	arg1	resistance					1288:1297	transepithelial electrical resistance	1261:1297	transepithelial electrical resistance	1261:1297	Tight junction barrier function was assessed by transepithelial electrical resistance, and cytokine levels were evaluated.					
31051086	8	71	dep	750	1382:1384	arg1	to					1379:1380	to	1379:1380	to	1379:1380	RESULTS Chondroitin sulfate at any dose up to 750 μg/ml was not associated with any statistically significant decrease in cell viability.					
31051086	3	72	theme	bacterial	637:645	arg1	interactions					647:658	bacterial interactions	637:658	bacterial interactions	637:658	Glycosaminoglycans, compounds naturally prevalent in both human milk and the intestine, are thought to be anti-inflammatory and capable of altering bacterial interactions within the gut.					
31051086	4	73	from	milk	819:822	arg1	class					782:786	the most prominent class	763:786	the most prominent class of glycosaminoglycans in human milk	763:822	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	4	73	from	milk	819:822	arg1	sulfate					754:760	chondroitin sulfate	742:760	chondroitin sulfate	742:760	RESEARCH AIM In this study, we aimed to evaluate the potential of chondroitin sulfate, the most prominent class of glycosaminoglycans in human milk, to protect against bacterial infection in an intestinal in vitro model.					
31051086	2	74	theme	integrity	382:390	arg1	dysfunction					392:402	intestinal integrity dysfunction	371:402	intestinal integrity dysfunction	371:402	Although the pathogenesis of necrotizing enterocolitis is yet to be solidified, intestinal integrity dysfunction, bacterial invasion and/or translocation, and inflammation may play important roles.					
31051086	1	75	theme	intestinal	225:234	arg1	enterocolitis					196:208	necrotizing enterocolitis	184:208	necrotizing enterocolitis	184:208	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	1	75	theme	intestinal	225:234	arg1	disease					249:255	a devastating intestinal inflammatory disease	211:255	a devastating intestinal inflammatory disease affecting the preterm population	211:288	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	7	76	theme	electrical	1277:1286	arg1	resistance					1288:1297	transepithelial electrical resistance	1261:1297	transepithelial electrical resistance	1261:1297	Tight junction barrier function was assessed by transepithelial electrical resistance, and cytokine levels were evaluated.					
31051086	7	77	theme	junction	1219:1226	arg1	function					1236:1243	Tight junction barrier function	1213:1243	Tight junction barrier function	1213:1243	Tight junction barrier function was assessed by transepithelial electrical resistance, and cytokine levels were evaluated.					
31051086	10	78	from	reduction	1799:1807	arg1	viability					1817:1825	cell viability	1812:1825	cell viability	1812:1825	CONCLUSIONS These data suggest chondroitin sulfate may protect against bacterial infection through a reduction in both invasion and translocation, importantly without attendant reduction in cell viability.					
31051086	2	79	theme	intestinal	371:380	arg1	dysfunction					392:402	intestinal integrity dysfunction	371:402	intestinal integrity dysfunction	371:402	Although the pathogenesis of necrotizing enterocolitis is yet to be solidified, intestinal integrity dysfunction, bacterial invasion and/or translocation, and inflammation may play important roles.					
31051086	1	80	theme	inflammatory	236:247	arg1	enterocolitis					196:208	necrotizing enterocolitis	184:208	necrotizing enterocolitis	184:208	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31051086	1	80	theme	inflammatory	236:247	arg1	disease					249:255	a devastating intestinal inflammatory disease	211:255	a devastating intestinal inflammatory disease affecting the preterm population	211:288	BACKGROUND Human milk is known to be protective against necrotizing enterocolitis, a devastating intestinal inflammatory disease affecting the preterm population.					
31356953	0	0	theme	improved	81:88	arg1	performance					101:111	improved hemostatic performance	81:111	improved hemostatic performance	81:111	Construction of a composite sponge containing tilapia peptides and chitosan with improved hemostatic performance.					
31356953	6	1	from	efficiency	1063:1072	arg1	models					1131:1136	New Zealand rabbit ear and femoral artery models	1089:1136	New Zealand rabbit ear and femoral artery models	1089:1136	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	1	2	theme	tilapia	163:169	arg1	peptide					171:177	tilapia peptide's	163:179	tilapia peptide's application as wounding dressing	163:212	Despite superior hemostasis and repair function, tilapia peptide's application as wounding dressing is greatly limited due to its poor biostability.					
31356953	7	3	theme	bleeding	1172:1179	arg1	time					1181:1184	the bleeding time	1168:1184	the bleeding time	1168:1184	Particularly, the bleeding time was shortened greatly, and the bleeding volume was reduced significantly.					
31356953	4	4	theme	hemorrhage	825:834	arg1	control					836:842	hemorrhage control	825:842	hemorrhage control	825:842	In addition, a new composite sponge using CS/TPM as fillers and CS as matrix was then constructed (S-CS/TPM) and used for hemorrhage control.					
31356953	3	5	theme	loading	495:501	arg1	peptides					528:535	the tilapia peptides	516:535	the tilapia peptides	516:535	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	3	5	theme	loading	495:501	arg1	%					492:492	10.6w%	487:492	10.6w% (loading capacity) of the tilapia peptides	487:535	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	3	5	theme	loading	495:501	arg1	capacity					503:510	loading capacity	495:510	loading capacity	495:510	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	6	6	theme	hemostatic	1052:1061	arg1	efficiency					1063:1072	the hemostatic efficiency	1048:1072	the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models	1048:1136	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	6	6	theme	hemostatic	1052:1061	arg1	higher					1146:1151	higher	1146:1151	higher	1146:1151	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	3	7	theme	improved	680:687	arg1	biostability					689:700	greatly improved biostability	672:700	greatly improved biostability	672:700	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	3	8	theme	tilapia	520:526	arg1	peptides					528:535	the tilapia peptides	516:535	the tilapia peptides	516:535	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	6	9	theme	rabbit	1101:1106	arg1	ear					1108:1110	Zealand rabbit ear	1093:1110	Zealand rabbit ear	1093:1110	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	2	10	with	chitosan	316:323	arg1	biodegradability					381:396	biodegradability	381:396	biodegradability	381:396	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	2	10	with	chitosan	316:323	arg1	biocompatibility					360:375	biocompatibility	360:375	biocompatibility	360:375	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	2	10	with	chitosan	316:323	arg1	activity					350:357	antibacterial activity	336:357	antibacterial activity	336:357	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	0	11	theme	hemostatic	90:99	arg1	performance					101:111	improved hemostatic performance	81:111	improved hemostatic performance	81:111	Construction of a composite sponge containing tilapia peptides and chitosan with improved hemostatic performance.					
31356953	3	12	dep	show	482:485	arg1	dispersed					554:562	dispersed	554:562	show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability	482:700	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	3	13	theme	peptides	528:535	arg1	peptides					528:535	the tilapia peptides	516:535	the tilapia peptides	516:535	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	3	13	theme	peptides	528:535	arg1	%					492:492	10.6w%	487:492	10.6w% (loading capacity) of the tilapia peptides	487:535	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	3	13	theme	peptides	528:535	arg1	capacity					503:510	loading capacity	495:510	loading capacity	495:510	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	6	14	theme	S-CS/TPM	1077:1084	arg1	efficiency					1063:1072	the hemostatic efficiency	1048:1072	the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models	1048:1136	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	6	14	theme	S-CS/TPM	1077:1084	arg1	higher					1146:1151	higher	1146:1151	higher	1146:1151	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	6	15	theme	Zealand	1093:1099	arg1	ear					1108:1110	Zealand rabbit ear	1093:1110	Zealand rabbit ear	1093:1110	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	0	16	theme	tilapia	46:52	arg1	peptides					54:61	tilapia peptides	46:61	tilapia peptides	46:61	Construction of a composite sponge containing tilapia peptides and chitosan with improved hemostatic performance.					
31356953	6	17	theme	artery	1124:1129	arg1	models					1131:1136	New Zealand rabbit ear and femoral artery models	1089:1136	New Zealand rabbit ear and femoral artery models	1089:1136	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	2	18	theme	ionic	446:450	arg1	method					462:467	ionic crosslink method	446:467	ionic crosslink method	446:467	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	6	19	theme	femoral	1116:1122	arg1	artery					1124:1129	femoral artery	1116:1129	femoral artery	1116:1129	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	4	20	theme	composite	722:730	arg1	sponge					732:737	a new composite sponge	716:737	a new composite sponge using CS/TPM as fillers and CS as matrix	716:778	In addition, a new composite sponge using CS/TPM as fillers and CS as matrix was then constructed (S-CS/TPM) and used for hemorrhage control.					
31356953	7	21	theme	bleeding	1217:1224	arg1	volume					1226:1231	the bleeding volume	1213:1231	the bleeding volume	1213:1231	Particularly, the bleeding time was shortened greatly, and the bleeding volume was reduced significantly.					
31356953	6	22	theme	CS	1034:1035	arg1	andCS/TPM					1037:1045	the CS andCS/TPM	1030:1045	the CS andCS/TPM	1030:1045	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	4	23	theme	new	718:720	arg1	sponge					732:737	a new composite sponge	716:737	a new composite sponge using CS/TPM as fillers and CS as matrix	716:778	In addition, a new composite sponge using CS/TPM as fillers and CS as matrix was then constructed (S-CS/TPM) and used for hemorrhage control.					
31356953	1	24	theme	wounding	196:203	arg1	dressing					205:212	wounding dressing	196:212	wounding dressing	196:212	Despite superior hemostasis and repair function, tilapia peptide's application as wounding dressing is greatly limited due to its poor biostability.					
31356953	0	25	theme	composite	18:26	arg1	sponge					28:33	a composite sponge	16:33	a composite sponge	16:33	Construction of a composite sponge containing tilapia peptides and chitosan with improved hemostatic performance.					
31356953	6	26	theme	ear	1108:1110	arg1	models					1131:1136	New Zealand rabbit ear and femoral artery models	1089:1136	New Zealand rabbit ear and femoral artery models	1089:1136	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	5	27	theme	blood	925:929	arg1	clotting					931:938	blood clotting	925:938	blood clotting	925:938	The results indicate that S-CS/TPM can absorb large volume of water, accelerate blood clotting, increase platelet adhesion and promote conversion of fibrinogen to fibrin.					
31356953	2	28	theme	tilapia	423:429	arg1	peptides					431:438	tilapia peptides	423:438	tilapia peptides using ionic crosslink method	423:467	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	5	29	theme	water	907:911	arg1	volume					897:902	large volume	891:902	large volume of water	891:911	The results indicate that S-CS/TPM can absorb large volume of water, accelerate blood clotting, increase platelet adhesion and promote conversion of fibrinogen to fibrin.					
31356953	2	30	theme	dressing	301:308	arg1	area					310:313	dressing area	301:313	dressing area	301:313	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	4	31	dep	constructed	789:799	arg1	S-CS/TPM					802:809	S-CS/TPM	802:809	S-CS/TPM	802:809	In addition, a new composite sponge using CS/TPM as fillers and CS as matrix was then constructed (S-CS/TPM) and used for hemorrhage control.					
31356953	0	32	theme	sponge	28:33	arg1	Construction					0:11	Construction	0:11	Construction of a composite sponge	0:33	Construction of a composite sponge containing tilapia peptides and chitosan with improved hemostatic performance.					
31356953	4	33	used	used	816:819	arg2	sponge					732:737	a new composite sponge	716:737	a new composite sponge using CS/TPM as fillers and CS as matrix	716:778	In addition, a new composite sponge using CS/TPM as fillers and CS as matrix was then constructed (S-CS/TPM) and used for hemorrhage control.					
31356953	3	34	dep	peptides	625:632	arg1	CS/TPM					648:653	CS/TPM	648:653	CS/TPM	648:653	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	3	34	dep	peptides	625:632	arg1	microspheres					634:645	microspheres	634:645	the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM)	590:654	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	5	35	theme	fibrinogen	994:1003	arg1	conversion					980:989	conversion	980:989	conversion of fibrinogen to fibrin	980:1013	The results indicate that S-CS/TPM can absorb large volume of water, accelerate blood clotting, increase platelet adhesion and promote conversion of fibrinogen to fibrin.					
31356953	2	36	used	used	403:406	arg2	chitosan					316:323	chitosan	316:323	chitosan (CS)	316:328	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	2	36	used	used	403:406	arg2	CS					326:327	CS	326:327	CS	326:327	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	1	37	theme	superior	122:129	arg1	hemostasis					131:140	superior hemostasis	122:140	superior hemostasis	122:140	Despite superior hemostasis and repair function, tilapia peptide's application as wounding dressing is greatly limited due to its poor biostability.					
31356953	2	38	theme	crosslink	452:460	arg1	method					462:467	ionic crosslink method	446:467	ionic crosslink method	446:467	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	6	39	theme	New	1089:1091	arg1	models					1131:1136	New Zealand rabbit ear and femoral artery models	1089:1136	New Zealand rabbit ear and femoral artery models	1089:1136	Compared with the CS andCS/TPM, the hemostatic efficiency of S-CS/TPM in New Zealand rabbit ear and femoral artery models is much higher.					
31356953	5	40	theme	platelet	950:957	arg1	adhesion					959:966	platelet adhesion	950:966	platelet adhesion	950:966	The results indicate that S-CS/TPM can absorb large volume of water, accelerate blood clotting, increase platelet adhesion and promote conversion of fibrinogen to fibrin.					
31356953	3	41	theme	chitosan/tilapia	608:623	arg1	peptides					625:632	the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM)	590:654	the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM)	590:654	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	5	42	theme	large	891:895	arg1	volume					897:902	large volume	891:902	large volume of water	891:911	The results indicate that S-CS/TPM can absorb large volume of water, accelerate blood clotting, increase platelet adhesion and promote conversion of fibrinogen to fibrin.					
31356953	3	43	theme	peptides	625:632	arg1	interior					578:585	interior	578:585	interior	578:585	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	3	43	theme	peptides	625:632	arg1	surface					567:573	surface	567:573	surface	567:573	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	1	44	theme	repair	146:151	arg1	function					153:160	repair function	146:160	repair function	146:160	Despite superior hemostasis and repair function, tilapia peptide's application as wounding dressing is greatly limited due to its poor biostability.					
31356953	2	45	theme	antibacterial	336:348	arg1	activity					350:357	antibacterial activity	336:357	antibacterial activity	336:357	To expand its application in wounding dressing area, chitosan (CS), with antibacterial activity, biocompatibility and biodegradability, was used to encapsulate tilapia peptides using ionic crosslink method.					
31356953	3	46	theme	as-fabricated	594:606	arg1	peptides					625:632	the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM)	590:654	the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM)	590:654	The results show 10.6w% (loading capacity) of the tilapia peptides are homogenously dispersed in surface or interior of the as-fabricated chitosan/tilapia peptides microspheres (CS/TPM), which leads to greatly improved biostability.					
31356953	1	47	theme	poor	244:247	arg1	biostability					249:260	its poor biostability	240:260	its poor biostability	240:260	Despite superior hemostasis and repair function, tilapia peptide's application as wounding dressing is greatly limited due to its poor biostability.					
31589419	0	0	theme	Polyunsaturated	98:112	arg1	Acids					120:124	Polyunsaturated Fatty Acids	98:124	Polyunsaturated Fatty Acids	98:124	pH-Responsive Emulsions with β-Cyclodextrin/Vitamin E Assembled Shells for Controlled Delivery of Polyunsaturated Fatty Acids.					
31589419	4	1	theme	sheetlike	713:721	arg1	shape					723:727	a uniform sheetlike shape	703:727	a uniform sheetlike shape	703:727	The resulting dual-functional particle presented a uniform sheetlike shape and nanometer size.					
31589419	1	2	theme	components	273:282	arg1	bioavailability					243:257	the increased bioavailability	229:257	the increased bioavailability of lipophilic components	229:282	Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals.					
31589419	7	3	from	pH	1171:1172	arg1	steady					1161:1166	steady	1161:1166	steady	1161:1166	Meanwhile, pH-induced protonation/deprotonation of carboxyl groups guaranteed that the emulsions kept steady at pH ≤4 but were unsteady under neutral conditions.					
31589419	2	4	theme	in	375:376	arg1	release					383:389	their in vivo release	369:389	their in vivo release	369:389	However, how to accurately control their in vivo release and stability is still challenging.					
31589419	9	5	from	pharmaceuticals	1546:1560	arg1	tract					1584:1588	a living digestive tract	1565:1588	a living digestive tract	1565:1588	This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.					
31589419	0	6	theme	Acids	120:124	arg1	Delivery					86:93	Controlled Delivery	75:93	Controlled Delivery of Polyunsaturated Fatty Acids	75:124	pH-Responsive Emulsions with β-Cyclodextrin/Vitamin E Assembled Shells for Controlled Delivery of Polyunsaturated Fatty Acids.					
31589419	5	7	dep	Fourier	847:853	arg1	transform					855:863	transform	855:863	transform infrared spectroscopy (FTIR)	855:892	In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).					
31589419	5	8	dep	transform	855:863	arg1	infrared					865:872	infrared	865:872	transform infrared spectroscopy (FTIR)	855:892	In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).					
31589419	8	9	from	acids	1376:1380	arg1	environment					1416:1426	the simulated intestine environment	1392:1426	the simulated intestine environment	1392:1426	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	6	10	theme	lipid	967:971	arg1	oxidation					973:981	lipid oxidation	967:981	lipid oxidation in the ODS-β-CD-VE-stabilized Pickering emulsion	967:1030	Benefiting from the antioxygenation of VE, lipid oxidation in the ODS-β-CD-VE-stabilized Pickering emulsion was effectively inhibited.					
31589419	0	11	theme	Fatty	114:118	arg1	Acids					120:124	Polyunsaturated Fatty Acids	98:124	Polyunsaturated Fatty Acids	98:124	pH-Responsive Emulsions with β-Cyclodextrin/Vitamin E Assembled Shells for Controlled Delivery of Polyunsaturated Fatty Acids.					
31589419	2	12	dep	in	375:376	arg1	vivo					378:381	vivo	378:381	vivo	378:381	However, how to accurately control their in vivo release and stability is still challenging.					
31589419	4	13	theme	uniform	705:711	arg1	shape					723:727	a uniform sheetlike shape	703:727	a uniform sheetlike shape	703:727	The resulting dual-functional particle presented a uniform sheetlike shape and nanometer size.					
31589419	3	14	theme	dual-functional	513:527	arg1	ODS-β-CD-VE					539:549	the dual-functional composite ODS-β-CD-VE	509:549	the dual-functional composite ODS-β-CD-VE	509:549	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	7	15	theme	groups	1119:1124	arg1	Meanwhile					1059:1067	Meanwhile	1059:1067	Meanwhile	1059:1067	Meanwhile, pH-induced protonation/deprotonation of carboxyl groups guaranteed that the emulsions kept steady at pH ≤4 but were unsteady under neutral conditions.					
31589419	7	15	theme	groups	1119:1124	arg1	protonation/deprotonation					1081:1105	pH-induced protonation/deprotonation	1070:1105	pH-induced protonation/deprotonation of carboxyl groups	1070:1124	Meanwhile, pH-induced protonation/deprotonation of carboxyl groups guaranteed that the emulsions kept steady at pH ≤4 but were unsteady under neutral conditions.					
31589419	9	16	theme	rational	1467:1474	arg1	construction					1490:1501	the rational and efficient construction	1463:1501	the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract	1463:1588	This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.					
31589419	8	17	theme	fatty	1370:1374	arg1	lipids					1238:1243	the lipids	1234:1243	the lipids contained in the emulsion	1234:1269	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	8	17	theme	fatty	1370:1374	arg1	PUFA					1383:1386	PUFA	1383:1386	PUFA	1383:1386	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	8	17	theme	fatty	1370:1374	arg1	acids					1376:1380	n-3 polyunsaturated fatty acids	1350:1380	n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment	1350:1426	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	3	18	theme	simple	455:460	arg1	esterification					462:475	the simple esterification	451:475	the simple esterification	451:475	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	3	19	theme	β-cyclodextrin	572:585	arg1	coassembly					558:567	the coassembly	554:567	the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE)	554:651	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	7	20	theme	neutral	1201:1207	arg1	conditions					1209:1218	neutral conditions	1201:1218	neutral conditions	1201:1218	Meanwhile, pH-induced protonation/deprotonation of carboxyl groups guaranteed that the emulsions kept steady at pH ≤4 but were unsteady under neutral conditions.					
31589419	5	21	theme	nuclear	813:819	arg1	NMR					841:843	NMR	841:843	NMR	841:843	In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).					
31589419	5	21	theme	nuclear	813:819	arg1	resonance					830:838	nuclear magnetic resonance	813:838	nuclear magnetic resonance (NMR)	813:844	In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).					
31589419	9	22	theme	living	1567:1572	arg1	tract					1584:1588	a living digestive tract	1565:1588	a living digestive tract	1565:1588	This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.					
31589419	3	23	theme	octadecenyl	595:605	arg1	ODSA					627:630	ODSA	627:630	ODSA	627:630	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	3	23	theme	octadecenyl	595:605	arg1	anhydride					616:624	octadecenyl succinic anhydride	595:624	octadecenyl succinic anhydride (ODSA)	595:631	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	0	24	theme	pH-Responsive	0:12	arg1	Emulsions					14:22	pH-Responsive Emulsions	0:22	pH-Responsive Emulsions with β-Cyclodextrin/Vitamin E	0:52	pH-Responsive Emulsions with β-Cyclodextrin/Vitamin E Assembled Shells for Controlled Delivery of Polyunsaturated Fatty Acids.					
31589419	6	25	theme	VE	963:964	arg1	antioxygenation					944:958	the antioxygenation	940:958	the antioxygenation of VE	940:964	Benefiting from the antioxygenation of VE, lipid oxidation in the ODS-β-CD-VE-stabilized Pickering emulsion was effectively inhibited.					
31589419	6	26	from	oxidation	973:981	arg1	emulsion					1023:1030	the ODS-β-CD-VE-stabilized Pickering emulsion	986:1030	the ODS-β-CD-VE-stabilized Pickering emulsion	986:1030	Benefiting from the antioxygenation of VE, lipid oxidation in the ODS-β-CD-VE-stabilized Pickering emulsion was effectively inhibited.					
31589419	3	27	theme	succinic	607:614	arg1	ODSA					627:630	ODSA	627:630	ODSA	627:630	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	3	27	theme	succinic	607:614	arg1	anhydride					616:624	octadecenyl succinic anhydride	595:624	octadecenyl succinic anhydride (ODSA)	595:631	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	1	28	theme	health	206:211	arg1	care					213:216	health care	206:216	health care	206:216	Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals.					
31589419	0	29	theme	β-Cyclodextrin/Vitamin	29:50	arg1	E					52:52	β-Cyclodextrin/Vitamin E	29:52	β-Cyclodextrin/Vitamin E	29:52	pH-Responsive Emulsions with β-Cyclodextrin/Vitamin E Assembled Shells for Controlled Delivery of Polyunsaturated Fatty Acids.					
31589419	5	30	theme	magnetic	821:828	arg1	NMR					841:843	NMR	841:843	NMR	841:843	In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).					
31589419	5	30	theme	magnetic	821:828	arg1	resonance					830:838	nuclear magnetic resonance	813:838	nuclear magnetic resonance (NMR)	813:844	In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).					
31589419	7	31	theme	pH-induced	1070:1079	arg1	Meanwhile					1059:1067	Meanwhile	1059:1067	Meanwhile	1059:1067	Meanwhile, pH-induced protonation/deprotonation of carboxyl groups guaranteed that the emulsions kept steady at pH ≤4 but were unsteady under neutral conditions.					
31589419	7	31	theme	pH-induced	1070:1079	arg1	protonation/deprotonation					1081:1105	pH-induced protonation/deprotonation	1070:1105	pH-induced protonation/deprotonation of carboxyl groups	1070:1124	Meanwhile, pH-induced protonation/deprotonation of carboxyl groups guaranteed that the emulsions kept steady at pH ≤4 but were unsteady under neutral conditions.					
31589419	9	32	from	supplements	1525:1535	arg1	tract					1584:1588	a living digestive tract	1565:1588	a living digestive tract	1565:1588	This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.					
31589419	1	33	theme	high-fat	318:325	arg1	meals					327:331	high-fat meals	318:331	high-fat meals	318:331	Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals.					
31589419	1	34	theme	Lipid-based	127:137	arg1	LBDSs					157:161	LBDSs	157:161	LBDSs	157:161	Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals.					
31589419	1	34	theme	Lipid-based	127:137	arg1	systems					148:154	Lipid-based delivery systems	127:154	Lipid-based delivery systems (LBDSs)	127:162	Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals.					
31589419	9	35	theme	even	1541:1544	arg1	pharmaceuticals					1546:1560	even pharmaceuticals	1541:1560	even pharmaceuticals in a living digestive tract	1541:1588	This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.					
31589419	7	36	theme	carboxyl	1110:1117	arg1	groups					1119:1124	carboxyl groups	1110:1124	carboxyl groups	1110:1124	Meanwhile, pH-induced protonation/deprotonation of carboxyl groups guaranteed that the emulsions kept steady at pH ≤4 but were unsteady under neutral conditions.					
31589419	8	37	theme	gastric	1291:1297	arg1	juice					1299:1303	gastric juice	1291:1303	gastric juice	1291:1303	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	4	38	theme	dual-functional	668:682	arg1	particle					684:691	The resulting dual-functional particle	654:691	The resulting dual-functional particle	654:691	The resulting dual-functional particle presented a uniform sheetlike shape and nanometer size.					
31589419	4	39	theme	resulting	658:666	arg1	particle					684:691	The resulting dual-functional particle	654:691	The resulting dual-functional particle	654:691	The resulting dual-functional particle presented a uniform sheetlike shape and nanometer size.					
31589419	9	40	theme	efficient	1480:1488	arg1	construction					1490:1501	the rational and efficient construction	1463:1501	the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract	1463:1588	This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.					
31589419	9	41	theme	digestive	1574:1582	arg1	tract					1584:1588	a living digestive tract	1565:1588	a living digestive tract	1565:1588	This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.					
31589419	5	42	theme	chemical	766:773	arg1	structure					775:783	its chemical structure	762:783	its chemical structure	762:783	In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).					
31589419	3	43	theme	anhydride	616:624	arg1	coassembly					558:567	the coassembly	554:567	the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE)	554:651	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	1	44	theme	delivery	139:146	arg1	LBDSs					157:161	LBDSs	157:161	LBDSs	157:161	Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals.					
31589419	1	44	theme	delivery	139:146	arg1	systems					148:154	Lipid-based delivery systems	127:154	Lipid-based delivery systems (LBDSs)	127:162	Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals.					
31589419	8	45	theme	intestine	1406:1414	arg1	environment					1416:1426	the simulated intestine environment	1392:1426	the simulated intestine environment	1392:1426	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	8	46	theme	simulated	1396:1404	arg1	environment					1416:1426	the simulated intestine environment	1392:1426	the simulated intestine environment	1392:1426	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	0	47	with	Emulsions	14:22	arg1	E					52:52	β-Cyclodextrin/Vitamin E	29:52	β-Cyclodextrin/Vitamin E	29:52	pH-Responsive Emulsions with β-Cyclodextrin/Vitamin E Assembled Shells for Controlled Delivery of Polyunsaturated Fatty Acids.					
31589419	3	48	theme	composite	529:537	arg1	ODS-β-CD-VE					539:549	the dual-functional composite ODS-β-CD-VE	509:549	the dual-functional composite ODS-β-CD-VE	509:549	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	3	49	theme	vitamin	638:644	arg1	VE					649:650	VE	649:650	VE	649:650	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	3	49	theme	vitamin	638:644	arg1	E					646:646	vitamin E	638:646	vitamin E (VE)	638:651	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	9	50	theme	LBDSs	1506:1510	arg1	construction					1490:1501	the rational and efficient construction	1463:1501	the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract	1463:1588	This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.					
31589419	1	51	theme	increased	233:241	arg1	bioavailability					243:257	the increased bioavailability	229:257	the increased bioavailability of lipophilic components	229:282	Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals.					
31589419	8	52	theme	polyunsaturated	1354:1368	arg1	lipids					1238:1243	the lipids	1234:1243	the lipids contained in the emulsion	1234:1269	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	8	52	theme	polyunsaturated	1354:1368	arg1	PUFA					1383:1386	PUFA	1383:1386	PUFA	1383:1386	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	8	52	theme	polyunsaturated	1354:1368	arg1	acids					1376:1380	n-3 polyunsaturated fatty acids	1350:1380	n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment	1350:1426	In this way, the lipids contained in the emulsion were protected from gastric juice and then digested and accurately released as n-3 polyunsaturated fatty acids (PUFA) in the simulated intestine environment.					
31589419	5	53	theme	X-ray	899:903	arg1	diffraction					905:915	X-ray diffraction	899:915	X-ray diffraction (XRD)	899:921	In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).					
31589419	5	53	theme	X-ray	899:903	arg1	XRD					918:920	XRD	918:920	XRD	918:920	In addition, its chemical structure was clarified in detail via nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD).					
31589419	3	54	theme	E	646:646	arg1	coassembly					558:567	the coassembly	554:567	the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE)	554:651	Here, after introducing the simple esterification and coprecipitation, we created the dual-functional composite ODS-β-CD-VE by the coassembly of β-cyclodextrin (β-CD), octadecenyl succinic anhydride (ODSA), and vitamin E (VE).					
31589419	4	55	theme	nanometer	733:741	arg1	size					743:746	nanometer size	733:746	nanometer size	733:746	The resulting dual-functional particle presented a uniform sheetlike shape and nanometer size.					
31589419	6	56	theme	Pickering	1013:1021	arg1	emulsion					1023:1030	the ODS-β-CD-VE-stabilized Pickering emulsion	986:1030	the ODS-β-CD-VE-stabilized Pickering emulsion	986:1030	Benefiting from the antioxygenation of VE, lipid oxidation in the ODS-β-CD-VE-stabilized Pickering emulsion was effectively inhibited.					
31589419	9	57	theme	nutrient	1516:1523	arg1	supplements					1525:1535	nutrient supplements	1516:1535	nutrient supplements	1516:1535	This strategy sheds some light on the rational and efficient construction of LBDSs for nutrient supplements and even pharmaceuticals in a living digestive tract.					
31589419	6	58	theme	ODS-β-CD-VE-stabilized	990:1011	arg1	emulsion					1023:1030	the ODS-β-CD-VE-stabilized Pickering emulsion	986:1030	the ODS-β-CD-VE-stabilized Pickering emulsion	986:1030	Benefiting from the antioxygenation of VE, lipid oxidation in the ODS-β-CD-VE-stabilized Pickering emulsion was effectively inhibited.					
31589419	0	59	theme	Controlled	75:84	arg1	Delivery					86:93	Controlled Delivery	75:93	Controlled Delivery of Polyunsaturated Fatty Acids	75:124	pH-Responsive Emulsions with β-Cyclodextrin/Vitamin E Assembled Shells for Controlled Delivery of Polyunsaturated Fatty Acids.					
31589419	1	60	theme	lipophilic	262:271	arg1	components					273:282	lipophilic components	262:282	lipophilic components	262:282	Lipid-based delivery systems (LBDSs) are widely applied in pharmaceuticals and health care because of the increased bioavailability of lipophilic components when they are coadministered with high-fat meals.					
31328701	8	0	theme	inulin	1015:1020	arg1	use					1008:1010	the use	1004:1010	the use of inulin in semi-skimmed sheep cheese	1004:1049	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	8	1	theme	whole	1123:1127	arg1	cheese					1140:1145	whole milk sheep cheese	1123:1145	whole milk sheep cheese	1123:1145	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	3	2	with	cheese	450:455	arg1	g					527:527	5 g/100 g	519:527	5 g/100 g inulin (SSCI)	519:541	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	0	3	with	cheese	31:36	arg1	inulin					43:48	inulin	43:48	inulin	43:48	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.					
31328701	8	4	theme	caloric	1199:1205	arg1	content					1207:1213	lower caloric content	1193:1213	lower caloric content	1193:1213	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	1	5	theme	Frescal	211:217	arg1	cheese					230:235	Frescal sheep milk cheese	211:235	Frescal sheep milk cheese	211:235	This research paper aimed to evaluate the role of inulin as a fat replacer on the quality of Frescal sheep milk cheese.					
31328701	6	6	theme	textural	785:792	arg1	adhesiveness					816:827	adhesiveness	816:827	adhesiveness	816:827	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	6	6	theme	textural	785:792	arg1	firmness					806:813	firmness	806:813	firmness	806:813	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	6	6	theme	textural	785:792	arg1	parameters					794:803	textural parameters	785:803	textural parameters (firmness, adhesiveness, cohesiveness, and gumminess)	785:857	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	6	6	theme	textural	785:792	arg1	gumminess					848:856	gumminess	848:856	gumminess	848:856	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	2	7	from	niche	285:289	arg1	industry					304:311	the dairy industry	294:311	the dairy industry	294:311	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	2	8	theme	dairy	298:302	arg1	industry					304:311	the dairy industry	294:311	the dairy industry	294:311	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	1	9	theme	sheep	219:223	arg1	cheese					230:235	Frescal sheep milk cheese	211:235	Frescal sheep milk cheese	211:235	This research paper aimed to evaluate the role of inulin as a fat replacer on the quality of Frescal sheep milk cheese.					
31328701	3	10	with	cheese	507:512	arg1	g					527:527	5 g/100 g	519:527	5 g/100 g inulin (SSCI)	519:541	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	1	11	theme	milk	225:228	arg1	cheese					230:235	Frescal sheep milk cheese	211:235	Frescal sheep milk cheese	211:235	This research paper aimed to evaluate the role of inulin as a fat replacer on the quality of Frescal sheep milk cheese.					
31328701	0	12	theme	technological	72:84	arg1	aspects					86:92	technological aspects	72:92	technological aspects	72:92	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.					
31328701	1	13	theme	research	123:130	arg1	paper					132:136	This research paper	118:136	This research paper	118:136	This research paper aimed to evaluate the role of inulin as a fat replacer on the quality of Frescal sheep milk cheese.					
31328701	0	14	theme	sensory	98:104	arg1	evaluation					106:115	sensory evaluation	98:115	sensory evaluation	98:115	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.					
31328701	6	15	theme	SSCI	764:767	arg1	cheeses					769:775	SSCI cheeses	764:775	SSCI cheeses	764:775	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	7	16	theme	sensory	971:977	arg1	analysis					979:986	sensory analysis	971:986	sensory analysis	971:986	All the formulations presented scores higher than 7.6 in sensory analysis.					
31328701	2	17	theme	Sheep	238:242	arg1	milk					244:247	Sheep milk	238:247	Sheep milk	238:247	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	2	17	theme	Sheep	238:242	arg1	derivatives					257:267	its derivatives	253:267	its derivatives	253:267	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	2	17	theme	Sheep	238:242	arg1	niche					285:289	a promising niche	273:289	a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products	273:383	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	1	18	theme	cheese	230:235	arg1	quality					200:206	the quality	196:206	the quality of Frescal sheep milk cheese	196:235	This research paper aimed to evaluate the role of inulin as a fat replacer on the quality of Frescal sheep milk cheese.					
31328701	5	19	theme	reduced	711:717	arg1	calorie					719:725	'reduced calorie'	710:726	'reduced calorie'	710:726	SSC and SSCI were considered 'reduced fat' or 'reduced calorie' products.					
31328701	3	20	theme	semi-skimmed	464:475	arg1	cheese					477:482	semi-skimmed cheese	464:482	semi-skimmed cheese (SSC)	464:488	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	20	theme	semi-skimmed	464:475	arg1	SSC					485:487	SSC	485:487	SSC	485:487	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	20	theme	semi-skimmed	464:475	arg1	formulations					418:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	21	theme	Frescal	392:398	arg1	cheese					477:482	semi-skimmed cheese	464:482	semi-skimmed cheese (SSC)	464:488	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	21	theme	Frescal	392:398	arg1	cheese					507:512	semi-skimmed cheese	494:512	semi-skimmed cheese with 5 g/100 g inulin (SSCI)	494:541	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	21	theme	Frescal	392:398	arg1	cheese					450:455	whole milk cheese	439:455	whole milk cheese (WMC)	439:461	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	21	theme	Frescal	392:398	arg1	formulations					418:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	0	22	theme	Frescal	12:18	arg1	cheese					31:36	Reduced-fat Frescal sheep milk cheese	0:36	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.	0:116	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.					
31328701	6	23	theme	inulin	754:759	arg1	addition					742:749	The addition	738:749	The addition of inulin to SSCI cheeses	738:775	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	8	24	theme	milk	1129:1132	arg1	cheese					1140:1145	whole milk sheep cheese	1123:1145	whole milk sheep cheese	1123:1145	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	0	25	theme	Reduced-fat	0:10	arg1	cheese					31:36	Reduced-fat Frescal sheep milk cheese	0:36	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.	0:116	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.					
31328701	3	26	theme	semi-skimmed	494:505	arg1	cheese					507:512	semi-skimmed cheese	494:512	semi-skimmed cheese with 5 g/100 g inulin (SSCI)	494:541	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	26	theme	semi-skimmed	494:505	arg1	formulations					418:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	27	dep	g	527:527	arg1	inulin					529:534	inulin	529:534	5 g/100 g inulin (SSCI)	519:541	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	27	dep	g	527:527	arg1	SSCI					537:540	SSCI	537:540	SSCI	537:540	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	2	28	theme	due	321:323	arg1	industry					304:311	the dairy industry	294:311	the dairy industry	294:311	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	0	29	theme	milk	26:29	arg1	cheese					31:36	Reduced-fat Frescal sheep milk cheese	0:36	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.	0:116	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.					
31328701	8	30	theme	sheep	1038:1042	arg1	cheese					1044:1049	semi-skimmed sheep cheese	1025:1049	semi-skimmed sheep cheese	1025:1049	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	2	31	theme	diversified	364:374	arg1	products					376:383	diversified products	364:383	diversified products	364:383	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	0	32	theme	sheep	20:24	arg1	cheese					31:36	Reduced-fat Frescal sheep milk cheese	0:36	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.	0:116	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.					
31328701	8	33	theme	lower	1193:1197	arg1	content					1207:1213	lower caloric content	1193:1213	lower caloric content	1193:1213	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	8	34	theme	sheep	1134:1138	arg1	cheese					1140:1145	whole milk sheep cheese	1123:1145	whole milk sheep cheese	1123:1145	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	1	35	theme	fat	180:182	arg1	replacer					184:191	a fat replacer	178:191	a fat replacer on the quality of Frescal sheep milk cheese	178:235	This research paper aimed to evaluate the role of inulin as a fat replacer on the quality of Frescal sheep milk cheese.					
31328701	1	36	theme	inulin	168:173	arg1	role					160:163	the role	156:163	the role of inulin as a fat replacer on the quality of Frescal sheep milk cheese	156:235	This research paper aimed to evaluate the role of inulin as a fat replacer on the quality of Frescal sheep milk cheese.					
31328701	2	37	theme	consumers	351:359	arg1	interest					339:346	interest	339:346	interest of consumers in diversified products	339:383	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	8	38	theme	cheese	1077:1082	arg1	production					1063:1072	the production	1059:1072	the production of cheese	1059:1082	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	6	39	theme	intermediate	864:875	arg1	characteristics					877:891	intermediate characteristics	864:891	intermediate characteristics between SSC and WMC	864:911	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	8	40	from	use	1008:1010	arg1	cheese					1044:1049	semi-skimmed sheep cheese	1025:1049	semi-skimmed sheep cheese	1025:1049	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	3	41	theme	milk	406:409	arg1	cheese					477:482	semi-skimmed cheese	464:482	semi-skimmed cheese (SSC)	464:488	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	41	theme	milk	406:409	arg1	cheese					507:512	semi-skimmed cheese	494:512	semi-skimmed cheese with 5 g/100 g inulin (SSCI)	494:541	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	41	theme	milk	406:409	arg1	cheese					450:455	whole milk cheese	439:455	whole milk cheese (WMC)	439:461	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	41	theme	milk	406:409	arg1	formulations					418:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	42	with	cheese	477:482	arg1	g					527:527	5 g/100 g	519:527	5 g/100 g inulin (SSCI)	519:541	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	8	43	theme	semi-skimmed	1025:1036	arg1	cheese					1044:1049	semi-skimmed sheep cheese	1025:1049	semi-skimmed sheep cheese	1025:1049	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	1	44	from	replacer	184:191	arg1	quality					200:206	the quality	196:206	the quality of Frescal sheep milk cheese	196:235	This research paper aimed to evaluate the role of inulin as a fat replacer on the quality of Frescal sheep milk cheese.					
31328701	3	45	theme	whole	439:443	arg1	WMC					458:460	WMC	458:460	WMC	458:460	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	45	theme	whole	439:443	arg1	cheese					450:455	whole milk cheese	439:455	whole milk cheese (WMC)	439:461	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	45	theme	whole	439:443	arg1	formulations					418:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	5	46	theme	reduced	694:700	arg1	fat					702:704	reduced fat'	694:705	reduced fat'	694:705	SSC and SSCI were considered 'reduced fat' or 'reduced calorie' products.					
31328701	0	47	dep	cheese	31:36	arg1	report					59:64	a first report	51:64	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.	0:116	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.					
31328701	2	48	from	interest	339:346	arg1	products					376:383	diversified products	364:383	diversified products	364:383	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	3	49	theme	milk	445:448	arg1	WMC					458:460	WMC	458:460	WMC	458:460	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	49	theme	milk	445:448	arg1	cheese					450:455	whole milk cheese	439:455	whole milk cheese (WMC)	439:461	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	49	theme	milk	445:448	arg1	formulations					418:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	6	50	dep	parameters	794:803	arg1	adhesiveness					816:827	adhesiveness	816:827	adhesiveness	816:827	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	6	50	dep	parameters	794:803	arg1	firmness					806:813	firmness	806:813	firmness	806:813	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	6	50	dep	parameters	794:803	arg1	parameters					794:803	textural parameters	785:803	textural parameters (firmness, adhesiveness, cohesiveness, and gumminess)	785:857	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	6	50	dep	parameters	794:803	arg1	cohesiveness					830:841	cohesiveness	830:841	cohesiveness	830:841	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	6	50	dep	parameters	794:803	arg1	gumminess					848:856	gumminess	848:856	gumminess	848:856	The addition of inulin to SSCI cheeses yielded textural parameters (firmness, adhesiveness, cohesiveness, and gumminess) with intermediate characteristics between SSC and WMC.					
31328701	8	51	theme	beneficial	1219:1228	arg1	characteristics					1230:1244	beneficial characteristics	1219:1244	beneficial characteristics valued by consumers	1219:1264	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	2	52	theme	promising	275:283	arg1	milk					244:247	Sheep milk	238:247	Sheep milk	238:247	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	2	52	theme	promising	275:283	arg1	derivatives					257:267	its derivatives	253:267	its derivatives	253:267	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	2	52	theme	promising	275:283	arg1	niche					285:289	a promising niche	273:289	a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products	273:383	Sheep milk and its derivatives are a promising niche in the dairy industry, mainly due to increasing interest of consumers in diversified products.					
31328701	0	53	theme	first	53:57	arg1	report					59:64	a first report	51:64	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.	0:116	Reduced-fat Frescal sheep milk cheese with inulin: a first report about technological aspects and sensory evaluation.					
31328701	8	54	with	foodstuff	1178:1186	arg1	content					1207:1213	lower caloric content	1193:1213	lower caloric content	1193:1213	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	8	54	with	foodstuff	1178:1186	arg1	characteristics					1230:1244	beneficial characteristics	1219:1244	beneficial characteristics valued by consumers	1219:1264	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	3	55	theme	sheep	400:404	arg1	cheese					477:482	semi-skimmed cheese	464:482	semi-skimmed cheese (SSC)	464:488	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	55	theme	sheep	400:404	arg1	cheese					507:512	semi-skimmed cheese	494:512	semi-skimmed cheese with 5 g/100 g inulin (SSCI)	494:541	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	55	theme	sheep	400:404	arg1	cheese					450:455	whole milk cheese	439:455	whole milk cheese (WMC)	439:461	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	55	theme	sheep	400:404	arg1	formulations					418:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	56	theme	cheese	411:416	arg1	cheese					477:482	semi-skimmed cheese	464:482	semi-skimmed cheese (SSC)	464:488	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	56	theme	cheese	411:416	arg1	cheese					507:512	semi-skimmed cheese	494:512	semi-skimmed cheese with 5 g/100 g inulin (SSCI)	494:541	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	56	theme	cheese	411:416	arg1	cheese					450:455	whole milk cheese	439:455	whole milk cheese (WMC)	439:461	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	3	56	theme	cheese	411:416	arg1	formulations					418:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations	386:429	Three Frescal sheep milk cheese formulations, namely whole milk cheese (WMC), semi-skimmed cheese (SSC) and semi-skimmed cheese with 5 g/100 g inulin (SSCI) were prepared.					
31328701	8	57	theme	similar	1112:1118	arg1	properties					1101:1110	properties	1101:1110	properties similar to whole milk sheep cheese	1101:1145	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
31328701	4	58	dep	fat	631:633	arg1	substitute					635:644	substitute	635:644	substitute	635:644	Their composition was evaluated and the feasibility of using inulin as a fat substitute was investigated.					
31328701	8	59	theme	foodstuff	1178:1186	arg1	development					1161:1171	the development	1157:1171	the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers	1157:1264	In conclusion, the use of inulin in semi-skimmed sheep cheese allowed the production of cheese with texturizing properties similar to whole milk sheep cheese, enabling the development of a foodstuff with lower caloric content and beneficial characteristics valued by consumers.					
30447356	0	0	theme	cationic	76:83	arg1	adsorption					89:98	cationic dye adsorption	76:98	cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites	76:168	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	5	1	theme	maximum	745:751	arg1	capacity					764:771	a maximum adsorption capacity	743:771	a maximum adsorption capacity of 905.5 mg·g-1	743:787	The Langmuir isotherm model exhibited a maximum adsorption capacity of 905.5 mg·g-1.					
30447356	1	2	theme	Magnetic	171:178	arg1	A					212:212	A	212:212	A	212:212	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	2	theme	Magnetic	171:178	arg1	AO-γ-Fe2O3					187:196	AO-γ-Fe2O3	187:196	AO-γ-Fe2O3	187:196	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	2	theme	Magnetic	171:178	arg1	beads					180:184	Magnetic beads	171:184	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs)	171:331	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	10	3	theme	adsorptive	1243:1252	arg1	loss					1266:1269	significant adsorptive performance loss	1231:1269	significant adsorptive performance loss	1231:1269	The AO-γ-Fe2O3 beads were used for six cycles without significant adsorptive performance loss.					
30447356	1	4	theme	maghemite	247:255	arg1	nanoparticles					257:269	citrate coated maghemite nanoparticles	232:269	citrate coated maghemite nanoparticles (γ-Fe2O3)	232:279	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	4	theme	maghemite	247:255	arg1	γ-Fe2O3					272:278	γ-Fe2O3	272:278	γ-Fe2O3	272:278	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	2	5	theme	γ-Fe2O3	465:471	arg1	10.24 nm					476:483	10.24 nm	476:483	10.24 nm	476:483	The XRD analysis revealed that the diameter of γ-Fe2O3 is 10.24 nm.					
30447356	2	5	theme	γ-Fe2O3	465:471	arg1	diameter					453:460	the diameter	449:460	the diameter of γ-Fe2O3	449:471	The XRD analysis revealed that the diameter of γ-Fe2O3 is 10.24 nm.					
30447356	9	6	theme	intraparticle	1146:1158	arg1	model					1170:1174	the intraparticle diffusion model	1142:1174	the intraparticle diffusion model	1142:1174	Adsorption kinetic data followed the intraparticle diffusion model.					
30447356	3	7	theme	mass	490:493	arg1	27.16					563:567	27.16	563:567	27.16	563:567	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3 were found to be 27.16 and 42.63 emu·g-1, respectively.					
30447356	3	7	theme	mass	490:493	arg1	magnetization					506:518	The mass saturation magnetization	486:518	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3	486:544	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3 were found to be 27.16 and 42.63 emu·g-1, respectively.					
30447356	8	8	from	nature	1101:1106	arg1	spontaneous					1070:1080	spontaneous	1070:1080	spontaneous	1070:1080	The thermodynamic parameters determined at 283, 293, 303, and 313 K revealed that the adsorption occurring was spontaneous and endothermic in nature.					
30447356	8	8	from	nature	1101:1106	arg1	occurring					1056:1064	the adsorption occurring	1041:1064	the adsorption occurring	1041:1064	The thermodynamic parameters determined at 283, 293, 303, and 313 K revealed that the adsorption occurring was spontaneous and endothermic in nature.					
30447356	0	9	theme	adsorption	89:98	arg1	thermodynamics					10:23	thermodynamics	10:23	thermodynamics	10:23	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	0	9	theme	adsorption	89:98	arg1	isotherms					38:46	equilibrium isotherms	26:46	equilibrium isotherms	26:46	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	0	9	theme	adsorption	89:98	arg1	Kinetics					0:7	Kinetics	0:7	Kinetics	0:7	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	0	9	theme	adsorption	89:98	arg1	studies					65:71	reusability studies	53:71	reusability studies	53:71	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	9	10	theme	diffusion	1160:1168	arg1	model					1170:1174	the intraparticle diffusion model	1142:1174	the intraparticle diffusion model	1142:1174	Adsorption kinetic data followed the intraparticle diffusion model.					
30447356	8	11	from	spontaneous	1070:1080	arg1	nature					1101:1106	nature	1101:1106	nature	1101:1106	The thermodynamic parameters determined at 283, 293, 303, and 313 K revealed that the adsorption occurring was spontaneous and endothermic in nature.					
30447356	0	12	theme	dye	85:87	arg1	adsorption					89:98	cationic dye adsorption	76:98	cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites	76:168	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	8	13	theme	thermodynamic	963:975	arg1	parameters					977:986	The thermodynamic parameters	959:986	The thermodynamic parameters determined at 283, 293, 303, and 313 K	959:1025	The thermodynamic parameters determined at 283, 293, 303, and 313 K revealed that the adsorption occurring was spontaneous and endothermic in nature.					
30447356	3	14	theme	γ-Fe2O3	538:544	arg1	27.16					563:567	27.16	563:567	27.16	563:567	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3 were found to be 27.16 and 42.63 emu·g-1, respectively.					
30447356	3	14	theme	γ-Fe2O3	538:544	arg1	magnetization					506:518	The mass saturation magnetization	486:518	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3	486:544	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3 were found to be 27.16 and 42.63 emu·g-1, respectively.					
30447356	0	15	theme	magnetic	103:110	arg1	composites					159:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	7	16	from	stability	919:927	arg1	pH					948:949	pH	948:949	pH (4-10)	948:956	The beads exhibited high adsorption stability in large domain of pH (4-10).					
30447356	7	16	from	stability	919:927	arg1	domain					938:943	large domain	932:943	large domain of pH (4-10)	932:956	The beads exhibited high adsorption stability in large domain of pH (4-10).					
30447356	11	17	theme	prepared	1300:1307	arg1	recyclable					1352:1361	recyclable	1352:1361	recyclable	1352:1361	Therefore, the eco-friendly prepared AO-γ-Fe2O3 beads were considered as highly recyclable and efficient adsorbent for methylene blue as they can be easily separated from water after treatment.					
30447356	11	17	theme	prepared	1300:1307	arg1	beads					1320:1324	the eco-friendly prepared AO-γ-Fe2O3 beads	1283:1324	the eco-friendly prepared AO-γ-Fe2O3 beads	1283:1324	Therefore, the eco-friendly prepared AO-γ-Fe2O3 beads were considered as highly recyclable and efficient adsorbent for methylene blue as they can be easily separated from water after treatment.					
30447356	11	17	theme	prepared	1300:1307	arg1	adsorbent					1377:1385	adsorbent	1377:1385	adsorbent	1377:1385	Therefore, the eco-friendly prepared AO-γ-Fe2O3 beads were considered as highly recyclable and efficient adsorbent for methylene blue as they can be easily separated from water after treatment.					
30447356	7	18	theme	pH	948:949	arg1	pH					948:949	pH	948:949	pH (4-10)	948:956	The beads exhibited high adsorption stability in large domain of pH (4-10).					
30447356	7	18	theme	pH	948:949	arg1	domain					938:943	large domain	932:943	large domain of pH (4-10)	932:956	The beads exhibited high adsorption stability in large domain of pH (4-10).					
30447356	10	19	used	used	1203:1206	arg2	beads					1192:1196	The AO-γ-Fe2O3 beads	1177:1196	The AO-γ-Fe2O3 beads	1177:1196	The AO-γ-Fe2O3 beads were used for six cycles without significant adsorptive performance loss.					
30447356	7	20	theme	large	932:936	arg1	pH					948:949	pH	948:949	pH (4-10)	948:956	The beads exhibited high adsorption stability in large domain of pH (4-10).					
30447356	7	20	theme	large	932:936	arg1	domain					938:943	large domain	932:943	large domain of pH (4-10)	932:956	The beads exhibited high adsorption stability in large domain of pH (4-10).					
30447356	3	21	theme	AO-γ-Fe2O3	523:532	arg1	27.16					563:567	27.16	563:567	27.16	563:567	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3 were found to be 27.16 and 42.63 emu·g-1, respectively.					
30447356	3	21	theme	AO-γ-Fe2O3	523:532	arg1	magnetization					506:518	The mass saturation magnetization	486:518	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3	486:544	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3 were found to be 27.16 and 42.63 emu·g-1, respectively.					
30447356	5	22	theme	isotherm	718:725	arg1	model					727:731	The Langmuir isotherm model	705:731	The Langmuir isotherm model	705:731	The Langmuir isotherm model exhibited a maximum adsorption capacity of 905.5 mg·g-1.					
30447356	1	23	theme	multiwalled	294:304	arg1	nanotubes					313:321	oxidized multiwalled carbon nanotubes	285:321	oxidized multiwalled carbon nanotubes (OMWCNTs)	285:331	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	23	theme	multiwalled	294:304	arg1	OMWCNTs					324:330	OMWCNTs	324:330	OMWCNTs	324:330	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	4	24	theme	isotherm	654:661	arg1	data					643:646	the data	639:646	the data of MB isotherm	639:661	The adsorption studies revealed that the data of MB isotherm were well fitted to the Freundlich model.					
30447356	11	25	theme	blue	1401:1404	arg1	methylene					1391:1399	methylene blue	1391:1404	methylene blue	1391:1404	Therefore, the eco-friendly prepared AO-γ-Fe2O3 beads were considered as highly recyclable and efficient adsorbent for methylene blue as they can be easily separated from water after treatment.					
30447356	4	26	theme	MB	651:652	arg1	isotherm					654:661	MB isotherm	651:661	MB isotherm	651:661	The adsorption studies revealed that the data of MB isotherm were well fitted to the Freundlich model.					
30447356	2	27	theme	XRD	422:424	arg1	analysis					426:433	The XRD analysis	418:433	The XRD analysis	418:433	The XRD analysis revealed that the diameter of γ-Fe2O3 is 10.24 nm.					
30447356	9	28	theme	kinetic	1120:1126	arg1	data					1128:1131	Adsorption kinetic data	1109:1131	Adsorption kinetic data	1109:1131	Adsorption kinetic data followed the intraparticle diffusion model.					
30447356	0	29	theme	multiwalled	130:140	arg1	composites					159:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	1	30	theme	alginate	202:209	arg1	A					212:212	A	212:212	A	212:212	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	30	theme	alginate	202:209	arg1	AO-γ-Fe2O3					187:196	AO-γ-Fe2O3	187:196	AO-γ-Fe2O3	187:196	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	30	theme	alginate	202:209	arg1	beads					180:184	Magnetic beads	171:184	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs)	171:331	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	31	theme	oxidized	285:292	arg1	nanotubes					313:321	oxidized multiwalled carbon nanotubes	285:321	oxidized multiwalled carbon nanotubes (OMWCNTs)	285:331	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	31	theme	oxidized	285:292	arg1	OMWCNTs					324:330	OMWCNTs	324:330	OMWCNTs	324:330	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	0	32	theme	alginate/oxidized	112:128	arg1	composites					159:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	1	33	used	used	354:357	arg2	beads					180:184	Magnetic beads	171:184	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs)	171:331	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	33	used	used	354:357	arg2	A					212:212	A	212:212	A	212:212	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	33	used	used	354:357	arg2	AO-γ-Fe2O3					187:196	AO-γ-Fe2O3	187:196	AO-γ-Fe2O3	187:196	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	0	34	theme	nanotubes	149:157	arg1	composites					159:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	10	35	theme	significant	1231:1241	arg1	loss					1266:1269	significant adsorptive performance loss	1231:1269	significant adsorptive performance loss	1231:1269	The AO-γ-Fe2O3 beads were used for six cycles without significant adsorptive performance loss.					
30447356	0	36	theme	equilibrium	26:36	arg1	isotherms					38:46	equilibrium isotherms	26:46	equilibrium isotherms	26:46	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	1	37	theme	carbon	306:311	arg1	nanotubes					313:321	oxidized multiwalled carbon nanotubes	285:321	oxidized multiwalled carbon nanotubes (OMWCNTs)	285:331	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	37	theme	carbon	306:311	arg1	OMWCNTs					324:330	OMWCNTs	324:330	OMWCNTs	324:330	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	0	38	theme	carbon	142:147	arg1	composites					159:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	magnetic alginate/oxidized multiwalled carbon nanotubes composites	103:168	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	5	39	theme	Langmuir	709:716	arg1	model					727:731	The Langmuir isotherm model	705:731	The Langmuir isotherm model	705:731	The Langmuir isotherm model exhibited a maximum adsorption capacity of 905.5 mg·g-1.					
30447356	4	40	theme	adsorption	606:615	arg1	studies					617:623	The adsorption studies	602:623	The adsorption studies	602:623	The adsorption studies revealed that the data of MB isotherm were well fitted to the Freundlich model.					
30447356	5	41	theme	905.5 mg·g-1	776:787	arg1	capacity					764:771	a maximum adsorption capacity	743:771	a maximum adsorption capacity of 905.5 mg·g-1	743:787	The Langmuir isotherm model exhibited a maximum adsorption capacity of 905.5 mg·g-1.					
30447356	1	42	theme	methylene	391:399	arg1	removal					380:386	the removal	376:386	the removal of methylene blue from water	376:415	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	10	43	theme	AO-γ-Fe2O3	1181:1190	arg1	beads					1192:1196	The AO-γ-Fe2O3 beads	1177:1196	The AO-γ-Fe2O3 beads	1177:1196	The AO-γ-Fe2O3 beads were used for six cycles without significant adsorptive performance loss.					
30447356	4	44	theme	Freundlich	687:696	arg1	model					698:702	the Freundlich model	683:702	the Freundlich model	683:702	The adsorption studies revealed that the data of MB isotherm were well fitted to the Freundlich model.					
30447356	8	45	theme	adsorption	1045:1054	arg1	spontaneous					1070:1080	spontaneous	1070:1080	spontaneous	1070:1080	The thermodynamic parameters determined at 283, 293, 303, and 313 K revealed that the adsorption occurring was spontaneous and endothermic in nature.					
30447356	8	45	theme	adsorption	1045:1054	arg1	occurring					1056:1064	the adsorption occurring	1041:1064	the adsorption occurring	1041:1064	The thermodynamic parameters determined at 283, 293, 303, and 313 K revealed that the adsorption occurring was spontaneous and endothermic in nature.					
30447356	1	46	theme	blue	401:404	arg1	methylene					391:399	methylene blue	391:404	methylene blue	391:404	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	11	47	theme	AO-γ-Fe2O3	1309:1318	arg1	recyclable					1352:1361	recyclable	1352:1361	recyclable	1352:1361	Therefore, the eco-friendly prepared AO-γ-Fe2O3 beads were considered as highly recyclable and efficient adsorbent for methylene blue as they can be easily separated from water after treatment.					
30447356	11	47	theme	AO-γ-Fe2O3	1309:1318	arg1	beads					1320:1324	the eco-friendly prepared AO-γ-Fe2O3 beads	1283:1324	the eco-friendly prepared AO-γ-Fe2O3 beads	1283:1324	Therefore, the eco-friendly prepared AO-γ-Fe2O3 beads were considered as highly recyclable and efficient adsorbent for methylene blue as they can be easily separated from water after treatment.					
30447356	11	47	theme	AO-γ-Fe2O3	1309:1318	arg1	adsorbent					1377:1385	adsorbent	1377:1385	adsorbent	1377:1385	Therefore, the eco-friendly prepared AO-γ-Fe2O3 beads were considered as highly recyclable and efficient adsorbent for methylene blue as they can be easily separated from water after treatment.					
30447356	6	48	theme	adsorbent	850:858	arg1	dose					860:863	adsorbent dose	850:863	adsorbent dose	850:863	The adsorption was very dependent on initial concentration, adsorbent dose, and temperature.					
30447356	7	49	theme	high	903:906	arg1	stability					919:927	high adsorption stability	903:927	high adsorption stability in large domain of pH (4-10)	903:956	The beads exhibited high adsorption stability in large domain of pH (4-10).					
30447356	6	50	theme	initial	827:833	arg1	concentration					835:847	initial concentration	827:847	initial concentration	827:847	The adsorption was very dependent on initial concentration, adsorbent dose, and temperature.					
30447356	9	51	theme	Adsorption	1109:1118	arg1	data					1128:1131	Adsorption kinetic data	1109:1131	Adsorption kinetic data	1109:1131	Adsorption kinetic data followed the intraparticle diffusion model.					
30447356	7	52	theme	adsorption	908:917	arg1	stability					919:927	high adsorption stability	903:927	high adsorption stability in large domain of pH (4-10)	903:956	The beads exhibited high adsorption stability in large domain of pH (4-10).					
30447356	5	53	theme	adsorption	753:762	arg1	capacity					764:771	a maximum adsorption capacity	743:771	a maximum adsorption capacity of 905.5 mg·g-1	743:787	The Langmuir isotherm model exhibited a maximum adsorption capacity of 905.5 mg·g-1.					
30447356	1	54	from	water	411:415	arg1	removal					380:386	the removal	376:386	the removal of methylene blue from water	376:415	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	3	55	theme	saturation	495:504	arg1	27.16					563:567	27.16	563:567	27.16	563:567	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3 were found to be 27.16 and 42.63 emu·g-1, respectively.					
30447356	3	55	theme	saturation	495:504	arg1	magnetization					506:518	The mass saturation magnetization	486:518	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3	486:544	The mass saturation magnetization of AO-γ-Fe2O3 and γ-Fe2O3 were found to be 27.16 and 42.63 emu·g-1, respectively.					
30447356	1	56	theme	citrate	232:238	arg1	nanoparticles					257:269	citrate coated maghemite nanoparticles	232:269	citrate coated maghemite nanoparticles (γ-Fe2O3)	232:279	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	56	theme	citrate	232:238	arg1	γ-Fe2O3					272:278	γ-Fe2O3	272:278	γ-Fe2O3	272:278	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	0	57	theme	reusability	53:63	arg1	studies					65:71	reusability studies	53:71	reusability studies	53:71	Kinetics, thermodynamics, equilibrium isotherms, and reusability studies of cationic dye adsorption by magnetic alginate/oxidized multiwalled carbon nanotubes composites.					
30447356	10	58	theme	performance	1254:1264	arg1	loss					1266:1269	significant adsorptive performance loss	1231:1269	significant adsorptive performance loss	1231:1269	The AO-γ-Fe2O3 beads were used for six cycles without significant adsorptive performance loss.					
30447356	1	59	theme	coated	240:245	arg1	nanoparticles					257:269	citrate coated maghemite nanoparticles	232:269	citrate coated maghemite nanoparticles (γ-Fe2O3)	232:279	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
30447356	1	59	theme	coated	240:245	arg1	γ-Fe2O3					272:278	γ-Fe2O3	272:278	γ-Fe2O3	272:278	Magnetic beads (AO-γ-Fe2O3) of alginate (A) impregnated with citrate coated maghemite nanoparticles (γ-Fe2O3) and oxidized multiwalled carbon nanotubes (OMWCNTs) were synthesized and used as adsorbent for the removal of methylene blue from water.					
31279890	0	0	theme	Au	88:89	arg1	recovery					76:83	selective recovery	66:83	selective recovery of Au(III) from the aqueous solution	66:120	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.					
31279890	7	1	theme	great	1090:1094	arg1	potential					1096:1104	great potential	1090:1104	great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity	1090:1201	CDF-CS has great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity.					
31279890	1	2	theme	magnetic	131:138	arg1	CDF-CS					160:165	CDF-CS	160:165	CDF-CS	160:165	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	1	2	theme	magnetic	131:138	arg1	chitosan					140:147	A novel magnetic chitosan adsorbent	123:157	A novel magnetic chitosan adsorbent (CDF-CS)	123:166	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	1	3	theme	crosslinked	225:235	arg1	compound					237:244	the crosslinked compound	221:244	the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution	221:357	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	3	4	theme	CDF-CS	709:714	arg1	capacity					697:704	the maximum adsorption capacity	674:704	the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0	674:764	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	4	theme	CDF-CS	709:714	arg1	capacity					630:637	a higher adsorption capacity	610:637	a higher adsorption capacity for Au(III) than the raw chitosan	610:671	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	5	from	range	569:573	arg1	large					553:557	large	553:557	large	553:557	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	5	from	range	569:573	arg1	capacity					525:532	the adsorption capacity	510:532	the adsorption capacity of CDF-CS	510:542	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	5	6	theme	adsorption	859:868	arg1	process					870:876	The adsorption process	855:876	The adsorption process of gold ions by CDF-CS	855:899	The adsorption process of gold ions by CDF-CS follows the Langmuir adsorption isotherm.					
31279890	6	7	theme	endothermic	1052:1062	arg1	chemisorption					1064:1076	an endothermic chemisorption	1049:1076	an endothermic chemisorption	1049:1076	Furthermore, the thermodynamic parameter indicates that the adsorption reaction of gold ions by CDF-CS is an endothermic chemisorption.					
31279890	6	7	theme	endothermic	1052:1062	arg1	reaction					1014:1021	the adsorption reaction	999:1021	the adsorption reaction of gold ions by CDF-CS	999:1044	Furthermore, the thermodynamic parameter indicates that the adsorption reaction of gold ions by CDF-CS is an endothermic chemisorption.					
31279890	3	8	theme	adsorption	686:695	arg1	capacity					697:704	the maximum adsorption capacity	674:704	the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0	674:764	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	8	theme	adsorption	686:695	arg1	capacity					630:637	a higher adsorption capacity	610:637	a higher adsorption capacity for Au(III) than the raw chitosan	610:671	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	9	theme	adsorption	619:628	arg1	capacity					697:704	the maximum adsorption capacity	674:704	the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0	674:764	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	9	theme	adsorption	619:628	arg1	capacity					630:637	a higher adsorption capacity	610:637	a higher adsorption capacity for Au(III) than the raw chitosan	610:671	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	8	10	theme	adsorption	1217:1226	arg1	mechanism					1228:1236	the adsorption mechanism	1213:1236	the adsorption mechanism	1213:1236	Finally, the adsorption mechanism is that chelation reaction and ion exchange mainly occurred between CDF-CS and Au(III).					
31279890	1	11	link	crosslinked	225:235	arg1	compound					237:244	the crosslinked compound	221:244	the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution	221:357	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	3	12	contain	has	606:608	arg2	capacity					630:637	a higher adsorption capacity	610:637	a higher adsorption capacity for Au(III) than the raw chitosan	610:671	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	12	contain	has	606:608	arg2	capacity					697:704	the maximum adsorption capacity	674:704	the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0	674:764	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	12	contain	has	606:608	arg1	large					553:557	large	553:557	large	553:557	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	12	contain	has	606:608	arg1	capacity					525:532	the adsorption capacity	510:532	the adsorption capacity of CDF-CS	510:542	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	13	from	large	553:557	arg1	range					569:573	a wide range	562:573	a wide range of pH values	562:586	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	1	14	theme	chitosan	249:256	arg1	compound					237:244	the crosslinked compound	221:244	the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution	221:357	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	6	15	theme	thermodynamic	960:972	arg1	parameter					974:982	the thermodynamic parameter	956:982	the thermodynamic parameter	956:982	Furthermore, the thermodynamic parameter indicates that the adsorption reaction of gold ions by CDF-CS is an endothermic chemisorption.					
31279890	1	16	theme	adsorbent	149:157	arg1	CDF-CS					160:165	CDF-CS	160:165	CDF-CS	160:165	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	1	16	theme	adsorbent	149:157	arg1	chitosan					140:147	A novel magnetic chitosan adsorbent	123:157	A novel magnetic chitosan adsorbent (CDF-CS)	123:166	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	9	17	from	recovery	1365:1372	arg1	promising					1352:1360	promising	1352:1360	promising	1352:1360	Therefore, CDF-CS is very promising in recovery of Au(III) from aqueous solutions.					
31279890	3	18	theme	maximum	678:684	arg1	capacity					697:704	the maximum adsorption capacity	674:704	the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0	674:764	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	18	theme	maximum	678:684	arg1	capacity					630:637	a higher adsorption capacity	610:637	a higher adsorption capacity for Au(III) than the raw chitosan	610:671	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	0	19	theme	pot	4:6	arg1	preparation					8:18	One pot preparation	0:18	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.	0:121	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.					
31279890	3	20	theme	higher	612:617	arg1	capacity					697:704	the maximum adsorption capacity	674:704	the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0	674:764	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	20	theme	higher	612:617	arg1	capacity					630:637	a higher adsorption capacity	610:637	a higher adsorption capacity for Au(III) than the raw chitosan	610:671	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	21	theme	adsorption	514:523	arg1	large					553:557	large	553:557	large	553:557	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	21	theme	adsorption	514:523	arg1	capacity					525:532	the adsorption capacity	510:532	the adsorption capacity of CDF-CS	510:542	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	2	22	theme	CDF-CS	409:414	arg1	characterization					364:379	characterization	364:379	characterization	364:379	The characterization and adsorption mechanism of CDF-CS were studied by SEM-EDS, VSM, FT-IR and XPS, respectively.					
31279890	2	22	theme	CDF-CS	409:414	arg1	mechanism					396:404	adsorption mechanism	385:404	adsorption mechanism	385:404	The characterization and adsorption mechanism of CDF-CS were studied by SEM-EDS, VSM, FT-IR and XPS, respectively.					
31279890	3	23	dep	7	599:599	arg1	to					596:597	to	596:597	to	596:597	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	24	dep	478.47 mg/g	732:742	arg1	III					723:725	III	723:725	III	723:725	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	6	25	theme	ions	1031:1034	arg1	chemisorption					1064:1076	an endothermic chemisorption	1049:1076	an endothermic chemisorption	1049:1076	Furthermore, the thermodynamic parameter indicates that the adsorption reaction of gold ions by CDF-CS is an endothermic chemisorption.					
31279890	6	25	theme	ions	1031:1034	arg1	reaction					1014:1021	the adsorption reaction	999:1021	the adsorption reaction of gold ions by CDF-CS	999:1044	Furthermore, the thermodynamic parameter indicates that the adsorption reaction of gold ions by CDF-CS is an endothermic chemisorption.					
31279890	3	26	theme	values	581:586	arg1	range					569:573	a wide range	562:573	a wide range of pH values	562:586	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	9	27	from	promising	1352:1360	arg1	recovery					1365:1372	recovery	1365:1372	recovery of Au(III) from aqueous solutions	1365:1406	Therefore, CDF-CS is very promising in recovery of Au(III) from aqueous solutions.					
31279890	0	28	theme	aqueous	105:111	arg1	solution					113:120	the aqueous solution	101:120	the aqueous solution	101:120	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.					
31279890	6	29	theme	gold	1026:1029	arg1	ions					1031:1034	gold ions	1026:1034	gold ions	1026:1034	Furthermore, the thermodynamic parameter indicates that the adsorption reaction of gold ions by CDF-CS is an endothermic chemisorption.					
31279890	5	30	theme	gold	881:884	arg1	ions					886:889	gold ions	881:889	gold ions	881:889	The adsorption process of gold ions by CDF-CS follows the Langmuir adsorption isotherm.					
31279890	2	31	theme	adsorption	385:394	arg1	mechanism					396:404	adsorption mechanism	385:404	adsorption mechanism	385:404	The characterization and adsorption mechanism of CDF-CS were studied by SEM-EDS, VSM, FT-IR and XPS, respectively.					
31279890	8	32	theme	chelation	1246:1254	arg1	reaction					1256:1263	chelation reaction	1246:1263	chelation reaction	1246:1263	Finally, the adsorption mechanism is that chelation reaction and ion exchange mainly occurred between CDF-CS and Au(III).					
31279890	3	33	theme	pH	578:579	arg1	values					581:586	pH values	578:586	pH values	578:586	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	0	34	theme	chitosan-cystamine	32:49	arg1	composites					51:60	magnetic chitosan-cystamine composites	23:60	magnetic chitosan-cystamine composites	23:60	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.					
31279890	1	35	theme	aqueous	342:348	arg1	solution					350:357	aqueous solution	342:357	aqueous solution	342:357	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	2	36	dep	characterization	364:379	arg1	The					360:362	The	360:362	The	360:362	The characterization and adsorption mechanism of CDF-CS were studied by SEM-EDS, VSM, FT-IR and XPS, respectively.					
31279890	9	37	from	solutions	1398:1406	arg1	recovery					1365:1372	recovery	1365:1372	recovery of Au(III) from aqueous solutions	1365:1406	Therefore, CDF-CS is very promising in recovery of Au(III) from aqueous solutions.					
31279890	7	38	theme	excellent	1163:1171	arg1	repeatability					1173:1185	the excellent repeatability	1159:1185	the excellent repeatability	1159:1185	CDF-CS has great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity.					
31279890	0	39	theme	magnetic	23:30	arg1	composites					51:60	magnetic chitosan-cystamine composites	23:60	magnetic chitosan-cystamine composites	23:60	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.					
31279890	1	40	theme	cystamine	267:275	arg1	compound					237:244	the crosslinked compound	221:244	the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution	221:357	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	5	41	theme	ions	886:889	arg1	process					870:876	The adsorption process	855:876	The adsorption process of gold ions by CDF-CS	855:899	The adsorption process of gold ions by CDF-CS follows the Langmuir adsorption isotherm.					
31279890	3	42	theme	raw	660:662	arg1	chitosan					664:671	the raw chitosan	656:671	the raw chitosan	656:671	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	4	43	theme	pseudo-second-order	820:838	arg1	model					848:852	this pseudo-second-order kinetic model	815:852	this pseudo-second-order kinetic model	815:852	The adsorption behavior is most consistent with this pseudo-second-order kinetic model.					
31279890	0	44	theme	composites	51:60	arg1	preparation					8:18	One pot preparation	0:18	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.	0:121	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.					
31279890	4	45	theme	adsorption	771:780	arg1	behavior					782:789	The adsorption behavior	767:789	The adsorption behavior	767:789	The adsorption behavior is most consistent with this pseudo-second-order kinetic model.					
31279890	4	45	theme	adsorption	771:780	arg1	consistent					799:808	consistent	799:808	consistent	799:808	The adsorption behavior is most consistent with this pseudo-second-order kinetic model.					
31279890	3	46	theme	CDF-CS	537:542	arg1	large					553:557	large	553:557	large	553:557	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	46	theme	CDF-CS	537:542	arg1	capacity					525:532	the adsorption capacity	510:532	the adsorption capacity of CDF-CS	510:542	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	7	47	contain	has	1086:1088	arg1	CDF-CS					1079:1084	CDF-CS	1079:1084	CDF-CS	1079:1084	CDF-CS has great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity.					
31279890	7	47	contain	has	1086:1088	arg2	potential					1096:1104	great potential	1090:1104	great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity	1090:1201	CDF-CS has great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity.					
31279890	6	48	theme	adsorption	1003:1012	arg1	chemisorption					1064:1076	an endothermic chemisorption	1049:1076	an endothermic chemisorption	1049:1076	Furthermore, the thermodynamic parameter indicates that the adsorption reaction of gold ions by CDF-CS is an endothermic chemisorption.					
31279890	6	48	theme	adsorption	1003:1012	arg1	reaction					1014:1021	the adsorption reaction	999:1021	the adsorption reaction of gold ions by CDF-CS	999:1044	Furthermore, the thermodynamic parameter indicates that the adsorption reaction of gold ions by CDF-CS is an endothermic chemisorption.					
31279890	1	49	theme	one-step	286:293	arg1	method					295:300	one-step method	286:300	one-step method for selectively recovering Au(III) from aqueous solution	286:357	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	8	50	theme	ion	1269:1271	arg1	exchange					1273:1280	ion exchange	1269:1280	ion exchange	1269:1280	Finally, the adsorption mechanism is that chelation reaction and ion exchange mainly occurred between CDF-CS and Au(III).					
31279890	1	51	theme	magnetic	197:204	arg1	particles					206:214	magnetic particles	197:214	magnetic particles	197:214	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	3	52	theme	experimental	479:490	arg1	results					492:498	The experimental results	475:498	The experimental results	475:498	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	5	53	theme	Langmuir	913:920	arg1	adsorption					922:931	the Langmuir adsorption	909:931	the Langmuir adsorption isotherm	909:940	The adsorption process of gold ions by CDF-CS follows the Langmuir adsorption isotherm.					
31279890	3	54	theme	wide	564:567	arg1	range					569:573	a wide range	562:573	a wide range of pH values	562:586	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	7	55	attach	removing	1110:1117	arg2	potential					1096:1104	great potential	1090:1104	great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity	1090:1201	CDF-CS has great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity.					
31279890	7	55	attach	removing	1110:1117	arg1	solutions					1142:1150	aqueous solutions	1134:1150	aqueous solutions	1134:1150	CDF-CS has great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity.					
31279890	7	56	theme	aqueous	1134:1140	arg1	solutions					1142:1150	aqueous solutions	1134:1150	aqueous solutions	1134:1150	CDF-CS has great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity.					
31279890	0	57	from	solution	113:120	arg1	recovery					76:83	selective recovery	66:83	selective recovery of Au(III) from the aqueous solution	66:120	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.					
31279890	4	58	theme	kinetic	840:846	arg1	model					848:852	this pseudo-second-order kinetic model	815:852	this pseudo-second-order kinetic model	815:852	The adsorption behavior is most consistent with this pseudo-second-order kinetic model.					
31279890	5	59	theme	adsorption	922:931	arg1	isotherm					933:940	the Langmuir adsorption isotherm	909:940	the Langmuir adsorption isotherm	909:940	The adsorption process of gold ions by CDF-CS follows the Langmuir adsorption isotherm.					
31279890	3	60	from	pH = 7.0	757:764	arg1	6 h					750:752	6 h	750:752	6 h at pH = 7.0	750:764	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	3	61	dep	large	553:557	arg1	7					599:599	7	599:599	7	599:599	The experimental results show that the adsorption capacity of CDF-CS is still large in a wide range of pH values (from 1 to 7) and has a higher adsorption capacity for Au(III) than the raw chitosan, the maximum adsorption capacity of CDF-CS for Au(III) was 478.47 mg/g about 6 h at pH = 7.0.					
31279890	9	62	theme	Au	1377:1378	arg1	recovery					1365:1372	recovery	1365:1372	recovery of Au(III) from aqueous solutions	1365:1406	Therefore, CDF-CS is very promising in recovery of Au(III) from aqueous solutions.					
31279890	9	63	theme	aqueous	1390:1396	arg1	solutions					1398:1406	aqueous solutions	1390:1406	aqueous solutions	1390:1406	Therefore, CDF-CS is very promising in recovery of Au(III) from aqueous solutions.					
31279890	0	64	theme	selective	66:74	arg1	recovery					76:83	selective recovery	66:83	selective recovery of Au(III) from the aqueous solution	66:120	One pot preparation of magnetic chitosan-cystamine composites for selective recovery of Au(III) from the aqueous solution.					
31279890	4	65	with	consistent	799:808	arg1	model					848:852	this pseudo-second-order kinetic model	815:852	this pseudo-second-order kinetic model	815:852	The adsorption behavior is most consistent with this pseudo-second-order kinetic model.					
31279890	1	66	theme	novel	125:129	arg1	CDF-CS					160:165	CDF-CS	160:165	CDF-CS	160:165	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	1	66	theme	novel	125:129	arg1	chitosan					140:147	A novel magnetic chitosan adsorbent	123:157	A novel magnetic chitosan adsorbent (CDF-CS)	123:166	A novel magnetic chitosan adsorbent (CDF-CS) was synthesized by inserting magnetic particles into the crosslinked compound of chitosan (CS) and cystamine based on one-step method for selectively recovering Au(III) from aqueous solution.					
31279890	7	67	theme	gold	1119:1122	arg1	ions					1124:1127	gold ions	1119:1127	gold ions	1119:1127	CDF-CS has great potential for removing gold ions from aqueous solutions due to the excellent repeatability and selectivity.					
31506050	0	0	theme	graphene	85:92	arg1	oxide					94:98	graphene oxide	85:98	graphene oxide composite membranes for guided bone regeneration	85:147	The physical, mechanical, and biological properties of silk fibroin/chitosan/reduced graphene oxide composite membranes for guided bone regeneration.					
31506050	2	1	theme	electron	553:560	arg1	SEM					574:576	SEM	574:576	SEM	574:576	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	2	1	theme	electron	553:560	arg1	microscopy					562:571	scanning electron microscopy	544:571	scanning electron microscopy (SEM) images	544:584	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	3	2	theme	osteogenic	649:658	arg1	differentiation					660:674	The osteogenic differentiation	645:674	The osteogenic differentiation	645:674	The osteogenic differentiation was determined by the alizarin red staining and alkaline phosphatase activity.					
31506050	1	3	theme	reduced	211:217	arg1	rGO					235:237	rGO	235:237	rGO	235:237	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	3	theme	reduced	211:217	arg1	oxide					228:232	reduced graphene oxide	211:232	reduced graphene oxide (rGO)	211:238	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	4	4	theme	content	867:873	arg1	increase					852:859	the increase	848:859	the increase of SF content	848:873	The results demonstrated that, the hydrophilicity, swelling and degradability decreases with the increase of SF content, whereas tensile strength increases accordingly.					
31506050	1	5	theme	solvent	297:303	arg1	method					313:318	solvent casting method	297:318	solvent casting method	297:318	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	2	6	theme	scanning	544:551	arg1	SEM					574:576	SEM	574:576	SEM	574:576	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	2	6	theme	scanning	544:551	arg1	microscopy					562:571	scanning electron microscopy	544:571	scanning electron microscopy (SEM) images	544:584	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	1	7	theme	graphene	219:226	arg1	rGO					235:237	rGO	235:237	rGO	235:237	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	7	theme	graphene	219:226	arg1	oxide					228:232	reduced graphene oxide	211:232	reduced graphene oxide (rGO)	211:238	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	0	8	theme	composite	100:108	arg1	membranes					110:118	graphene oxide composite membranes	85:118	graphene oxide composite membranes for guided bone regeneration	85:147	The physical, mechanical, and biological properties of silk fibroin/chitosan/reduced graphene oxide composite membranes for guided bone regeneration.					
31506050	1	9	theme	casting	305:311	arg1	method					313:318	solvent casting method	297:318	solvent casting method	297:318	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	2	10	theme	DAPI	590:593	arg1	assay					638:642	DAPI (4', 6-diamidoino-2-phenylindole) staining assay	590:642	DAPI (4', 6-diamidoino-2-phenylindole) staining assay	590:642	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	7	11	theme	weight	1363:1368	arg1	ratio					1370:1374	84:7:9 weight ratio	1356:1374	84:7:9 weight ratio	1356:1374	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	2	12	dep	DAPI	590:593	arg1	4					596:596	4	596:596	4	596:596	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	2	12	dep	DAPI	590:593	arg1	6-diamidoino-2-phenylindole					600:626	6-diamidoino-2-phenylindole	600:626	6-diamidoino-2-phenylindole	600:626	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	1	13	theme	oxide	228:232	arg1	blends					165:170	blends	165:170	blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes	165:276	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	0	14	theme	oxide	94:98	arg1	membranes					110:118	graphene oxide composite membranes	85:118	graphene oxide composite membranes for guided bone regeneration	85:147	The physical, mechanical, and biological properties of silk fibroin/chitosan/reduced graphene oxide composite membranes for guided bone regeneration.					
31506050	2	15	theme	SF	469:470	arg1	influence					452:460	The influence	448:460	The influence of CS, SF, and rGO on osteoblasts spreading and attachment	448:519	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	1	16	theme	mechanical	407:416	arg1	features					344:351	their architectural features	324:351	their architectural features	324:351	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	16	theme	mechanical	407:416	arg1	properties					418:427	mechanical properties	407:427	mechanical properties	407:427	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	6	17	theme	CS	1132:1133	arg1	content					1135:1141	the CS content	1128:1141	the CS content	1128:1141	The cell behaviors of G-292 cells were enhanced by increasing the CS content.					
31506050	7	18	theme	optimum	1281:1287	arg1	results					1289:1295	optimum results	1281:1295	optimum results	1281:1295	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	2	19	dep	osteoblasts	484:494	arg1	osteoblasts					484:494	osteoblasts spreading and attachment	484:519	osteoblasts spreading and attachment	484:519	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	2	19	dep	osteoblasts	484:494	arg1	attachment					510:519	attachment	510:519	attachment	510:519	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	2	19	dep	osteoblasts	484:494	arg1	spreading					496:504	spreading	496:504	spreading	496:504	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	7	20	with	SF	1340:1341	arg1	ratio					1370:1374	84:7:9 weight ratio	1356:1374	84:7:9 weight ratio	1356:1374	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	7	21	theme	84:7:9	1356:1361	arg1	ratio					1370:1374	84:7:9 weight ratio	1356:1374	84:7:9 weight ratio	1356:1374	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	0	22	theme	physical	4:11	arg1	properties					41:50	The physical, mechanical, and biological properties	0:50	The physical, mechanical, and biological properties of silk	0:58	The physical, mechanical, and biological properties of silk fibroin/chitosan/reduced graphene oxide composite membranes for guided bone regeneration.					
31506050	1	23	theme	architectural	330:342	arg1	hydrophilicity					354:367	hydrophilicity	354:367	hydrophilicity	354:367	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	23	theme	architectural	330:342	arg1	degradation					390:400	degradation	390:400	degradation	390:400	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	23	theme	architectural	330:342	arg1	porosity					370:377	porosity	370:377	porosity	370:377	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	23	theme	architectural	330:342	arg1	swelling					380:387	swelling	380:387	swelling	380:387	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	23	theme	architectural	330:342	arg1	properties					418:427	mechanical properties	407:427	mechanical properties	407:427	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	23	theme	architectural	330:342	arg1	features					344:351	their architectural features	324:351	their architectural features	324:351	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	2	24	theme	CS	465:466	arg1	influence					452:460	The influence	448:460	The influence of CS, SF, and rGO on osteoblasts spreading and attachment	448:519	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	1	25	theme	chitosan	175:182	arg1	SF					203:204	SF	203:204	SF	203:204	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	25	theme	chitosan	175:182	arg1	fibroin					194:200	chitosan (CS)/silk fibroin	175:200	chitosan (CS)/silk fibroin (SF)	175:205	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	2	26	theme	microscopy	562:571	arg1	images					579:584	scanning electron microscopy (SEM) images	544:584	scanning electron microscopy (SEM) images	544:584	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	7	27	dep	SF	1340:1341	arg1	rGO					1347:1349	rGO	1347:1349	SF:CS: rGO with 84:7:9 weight ratio	1340:1374	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	7	27	dep	SF	1340:1341	arg1	CS					1343:1344	CS	1343:1344	SF:CS: rGO with 84:7:9 weight ratio	1340:1374	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	0	28	theme	mechanical	14:23	arg1	properties					41:50	The physical, mechanical, and biological properties	0:50	The physical, mechanical, and biological properties of silk	0:58	The physical, mechanical, and biological properties of silk fibroin/chitosan/reduced graphene oxide composite membranes for guided bone regeneration.					
31506050	3	29	theme	alizarin	698:705	arg1	staining					711:718	the alizarin red staining	694:718	the alizarin red staining	694:718	The osteogenic differentiation was determined by the alizarin red staining and alkaline phosphatase activity.					
31506050	0	30	theme	bone	131:134	arg1	regeneration					136:147	guided bone regeneration	124:147	guided bone regeneration	124:147	The physical, mechanical, and biological properties of silk fibroin/chitosan/reduced graphene oxide composite membranes for guided bone regeneration.					
31506050	7	31	theme	bone	1253:1256	arg1	engineering					1265:1275	bone tissue engineering	1253:1275	bone tissue engineering	1253:1275	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	0	32	theme	guided	124:129	arg1	regeneration					136:147	guided bone regeneration	124:147	guided bone regeneration	124:147	The physical, mechanical, and biological properties of silk fibroin/chitosan/reduced graphene oxide composite membranes for guided bone regeneration.					
31506050	2	33	from	influence	452:460	arg1	osteoblasts					484:494	osteoblasts spreading and attachment	484:519	osteoblasts spreading and attachment	484:519	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	2	33	from	influence	452:460	arg1	attachment					510:519	attachment	510:519	attachment	510:519	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	2	33	from	influence	452:460	arg1	spreading					496:504	spreading	496:504	spreading	496:504	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	7	34	theme	tissue	1258:1263	arg1	engineering					1265:1275	bone tissue engineering	1253:1275	bone tissue engineering	1253:1275	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	1	35	theme	guided	243:248	arg1	regeneration					255:266	guided bone regeneration	243:266	guided bone regeneration membranes	243:276	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	4	36	theme	tensile	884:890	arg1	strength					892:899	tensile strength	884:899	tensile strength	884:899	The results demonstrated that, the hydrophilicity, swelling and degradability decreases with the increase of SF content, whereas tensile strength increases accordingly.					
31506050	1	37	theme	/silk	188:192	arg1	SF					203:204	SF	203:204	SF	203:204	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	37	theme	/silk	188:192	arg1	fibroin					194:200	chitosan (CS)/silk fibroin	175:200	chitosan (CS)/silk fibroin (SF)	175:205	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	4	38	dep	decreases	833:841	arg1	whereas					876:882	whereas	876:882	whereas	876:882	The results demonstrated that, the hydrophilicity, swelling and degradability decreases with the increase of SF content, whereas tensile strength increases accordingly.					
31506050	1	39	theme	bone	250:253	arg1	regeneration					255:266	guided bone regeneration	243:266	guided bone regeneration membranes	243:276	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	2	40	theme	rGO	477:479	arg1	influence					452:460	The influence	448:460	The influence of CS, SF, and rGO on osteoblasts spreading and attachment	448:519	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	1	41	theme	fibroin	194:200	arg1	blends					165:170	blends	165:170	blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes	165:276	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	1	42	theme	regeneration	255:266	arg1	membranes					268:276	guided bone regeneration membranes	243:276	guided bone regeneration membranes	243:276	In this study, blends of chitosan (CS)/silk fibroin (SF) and reduced graphene oxide (rGO) as guided bone regeneration membranes were fabricated by solvent casting method and their architectural features, hydrophilicity, porosity, swelling, degradation, and mechanical properties were investigated.					
31506050	6	43	theme	cells	1094:1098	arg1	behaviors					1075:1083	The cell behaviors	1066:1083	The cell behaviors of G-292 cells	1066:1098	The cell behaviors of G-292 cells were enhanced by increasing the CS content.					
31506050	0	44	theme	biological	30:39	arg1	properties					41:50	The physical, mechanical, and biological properties	0:50	The physical, mechanical, and biological properties of silk	0:58	The physical, mechanical, and biological properties of silk fibroin/chitosan/reduced graphene oxide composite membranes for guided bone regeneration.					
31506050	3	45	theme	red	707:709	arg1	staining					711:718	the alizarin red staining	694:718	the alizarin red staining	694:718	The osteogenic differentiation was determined by the alizarin red staining and alkaline phosphatase activity.					
31506050	2	46	theme	staining	629:636	arg1	assay					638:642	DAPI (4', 6-diamidoino-2-phenylindole) staining assay	590:642	DAPI (4', 6-diamidoino-2-phenylindole) staining assay	590:642	The influence of CS, SF, and rGO on osteoblasts spreading and attachment was also determined by scanning electron microscopy (SEM) images and DAPI (4', 6-diamidoino-2-phenylindole) staining assay.					
31506050	6	47	theme	G-292	1088:1092	arg1	cells					1094:1098	G-292 cells	1088:1098	G-292 cells	1088:1098	The cell behaviors of G-292 cells were enhanced by increasing the CS content.					
31506050	4	48	theme	SF	864:865	arg1	content					867:873	SF content	864:873	SF content	864:873	The results demonstrated that, the hydrophilicity, swelling and degradability decreases with the increase of SF content, whereas tensile strength increases accordingly.					
31506050	5	49	theme	tensile	1002:1008	arg1	strength					1010:1017	tensile strength	1002:1017	tensile strength	1002:1017	It was confirmed that with increasing the rGO concentration, the porosity and tensile strength decreased but the hydrophilicity was improved.					
31506050	7	50	theme	blended	1206:1212	arg1	membranes					1214:1222	SF/CS/rGO blended membranes	1196:1222	SF/CS/rGO blended membranes	1196:1222	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	7	50	theme	blended	1206:1212	arg1	candidates					1238:1247	promising candidates	1228:1247	promising candidates for bone tissue engineering	1228:1275	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	7	51	theme	promising	1228:1236	arg1	membranes					1214:1222	SF/CS/rGO blended membranes	1196:1222	SF/CS/rGO blended membranes	1196:1222	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	7	51	theme	promising	1228:1236	arg1	candidates					1238:1247	promising candidates	1228:1247	promising candidates for bone tissue engineering	1228:1275	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	8	52	theme	optimized	1405:1413	arg1	membranes					1415:1423	the optimized membranes	1401:1423	the optimized membranes	1401:1423	It should be noted that the optimized membranes should be further studied for clinical applications.					
31506050	0	53	theme	silk	55:58	arg1	properties					41:50	The physical, mechanical, and biological properties	0:50	The physical, mechanical, and biological properties of silk	0:58	The physical, mechanical, and biological properties of silk fibroin/chitosan/reduced graphene oxide composite membranes for guided bone regeneration.					
31506050	8	54	theme	clinical	1455:1462	arg1	applications					1464:1475	clinical applications	1455:1475	clinical applications	1455:1475	It should be noted that the optimized membranes should be further studied for clinical applications.					
31506050	6	55	theme	cell	1070:1073	arg1	behaviors					1075:1083	The cell behaviors	1066:1083	The cell behaviors of G-292 cells	1066:1098	The cell behaviors of G-292 cells were enhanced by increasing the CS content.					
31506050	3	56	theme	phosphatase	733:743	arg1	activity					745:752	alkaline phosphatase activity	724:752	alkaline phosphatase activity	724:752	The osteogenic differentiation was determined by the alizarin red staining and alkaline phosphatase activity.					
31506050	5	57	theme	rGO	966:968	arg1	concentration					970:982	the rGO concentration	962:982	the rGO concentration	962:982	It was confirmed that with increasing the rGO concentration, the porosity and tensile strength decreased but the hydrophilicity was improved.					
31506050	7	58	theme	SF/CS/rGO	1196:1204	arg1	membranes					1214:1222	SF/CS/rGO blended membranes	1196:1222	SF/CS/rGO blended membranes	1196:1222	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	7	58	theme	SF/CS/rGO	1196:1204	arg1	candidates					1238:1247	promising candidates	1228:1247	promising candidates for bone tissue engineering	1228:1275	According to the results, it can be concluded that, SF/CS/rGO blended membranes are promising candidates for bone tissue engineering and optimum results were obtained for the membrane composed of SF:CS: rGO with 84:7:9 weight ratio.					
31506050	3	59	theme	alkaline	724:731	arg1	activity					745:752	alkaline phosphatase activity	724:752	alkaline phosphatase activity	724:752	The osteogenic differentiation was determined by the alizarin red staining and alkaline phosphatase activity.					
30060077	13	0	theme	Solobacterium	1923:1935	arg1	abundance					1887:1895	a lower relative abundance	1870:1895	a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05)	1870:1976	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	15	1	theme	HF-LS	2162:2166	arg1	diet					2168:2171	the HF-LS diet	2158:2171	the HF-LS diet	2158:2171	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	3	2	theme	crossover	769:777	arg1	arrangement					779:789	a 2-period crossover arrangement	758:789	a 2-period crossover arrangement	758:789	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	7	3	theme	Fecal	1107:1111	arg1	samples					1113:1119	Fecal samples	1107:1119	Fecal samples	1107:1119	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	4	4	theme	fat	813:815	arg1	fat					813:815	fat	813:815	fat	813:815	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	4	4	theme	fat	813:815	arg1	amount					803:808	The higher amount	792:808	The higher amount of fat in the HF-LS diet	792:833	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	3	5	theme	%	575:575	arg1	ME					577:578	25% ME	573:578	25% ME	573:578	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	4	6	theme	LF-HS	900:904	arg1	diet					906:909	the LF-HS diet	896:909	the LF-HS diet	896:909	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	1	7	theme	high-fat	216:223	arg1	diets					225:229	high-fat diets	216:229	high-fat diets	216:229	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	15	8	theme	dogs	2149:2152	arg1	feces					2140:2144	feces	2140:2144	feces of dogs fed the HF-LS diet	2140:2171	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	13	9	dep	fed	1983:1985	arg1	of					1987:1988	of	1987:1988	of	1987:1988	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	11	10	theme	HF-LS	1676:1680	arg1	diet					1682:1685	the HF-LS diet	1672:1685	the HF-LS diet	1672:1685	Feeding the HF-LS diet led to a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet.					
30060077	4	11	theme	higher	869:874	arg1	starch					886:891	starch	886:891	starch	886:891	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	4	11	theme	higher	869:874	arg1	amount					876:881	the higher amount	865:881	the higher amount of starch in the LF-HS diet	865:909	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	13	12	theme	˂	1970:1970	arg1	P					1968:1968	P ˂ 0.05	1968:1975	P ˂ 0.05	1968:1975	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	13	12	theme	˂	1970:1970	arg1	Coprobacillus					1953:1965	Coprobacillus	1953:1965	Coprobacillus (P ˂ 0.05)	1953:1976	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	15	13	theme	LF-HS	2091:2095	arg1	diet					2097:2100	the LF-HS diet	2087:2100	the LF-HS diet	2087:2100	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	15	14	theme	P	2199:2199	arg1	h					2196:2196	6 h	2194:2196	6 h (P < 0.01)	2194:2207	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	15	14	theme	P	2199:2199	arg1	<					2201:2201	P < 0.01	2199:2206	P < 0.01	2199:2206	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	3	15	dep	dogs	496:499	arg1	yr					471:472	3 to 7 yr	464:472	3 to 7 yr of age	464:479	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	0	16	from	composition	60:70	arg1	dogs1					88:92	dogs1	88:92	dogs1	88:92	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.					
30060077	10	17	theme	blood	1549:1553	arg1	samples					1555:1561	blood samples	1549:1561	blood samples	1549:1561	On week 7, blood samples were collected at 0- and 180-min postfeeding for the analysis of bacterial lipopolysaccharide (LPS).					
30060077	1	18	from	impact	206:211	arg1	microbiota					245:254	canine gut microbiota	234:254	canine gut microbiota	234:254	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	4	19	theme	higher	796:801	arg1	fat					813:815	fat	813:815	fat	813:815	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	4	19	theme	higher	796:801	arg1	amount					803:808	The higher amount	792:808	The higher amount of fat in the HF-LS diet	792:833	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	7	20	theme	bile	1280:1283	arg1	acids					1285:1289	bile acids	1280:1289	bile acids	1280:1289	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	9	21	theme	incubation	1526:1535	arg1	h					1521:1521	2, 4, 6, 9, 12, and 24 h	1498:1521	2, 4, 6, 9, 12, and 24 h of incubation	1498:1535	Gas production was measured at 2, 4, 6, 9, 12, and 24 h of incubation.					
30060077	3	22	theme	age	477:479	arg1	yr					471:472	3 to 7 yr	464:472	3 to 7 yr of age	464:479	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	14	23	theme	VFA	2024:2026	arg1	contents					2028:2035	Fecal ammonia and VFA contents	2006:2035	Fecal ammonia and VFA contents	2006:2035	Fecal ammonia and VFA contents were not affected by diet (P > 0.10).					
30060077	15	24	theme	less	2182:2185	arg1	gas					2187:2189	less gas	2182:2189	less gas	2182:2189	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	3	25	dep	diet	653:656	arg1	HF-LS					659:663	HF-LS	659:663	HF-LS	659:663	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	7	26	theme	next-generation	1251:1265	arg1	sequencing					1267:1276	composition [by Ion-Torrent next-generation sequencing	1223:1276	composition [by Ion-Torrent next-generation sequencing	1223:1276	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	10	27	theme	bacterial	1628:1636	arg1	lipopolysaccharide					1638:1655	bacterial lipopolysaccharide	1628:1655	bacterial lipopolysaccharide (LPS)	1628:1661	On week 7, blood samples were collected at 0- and 180-min postfeeding for the analysis of bacterial lipopolysaccharide (LPS).					
30060077	10	27	theme	bacterial	1628:1636	arg1	LPS					1658:1660	LPS	1658:1660	LPS	1658:1660	On week 7, blood samples were collected at 0- and 180-min postfeeding for the analysis of bacterial lipopolysaccharide (LPS).					
30060077	0	28	theme	fecal	43:47	arg1	composition					60:70	fecal microbiota composition	43:70	fecal microbiota composition	43:70	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.					
30060077	17	29	dep	Prevotella	2475:2484	arg1	fermentation					2496:2507	fermentation	2496:2507	fermentation	2496:2507	These findings indicate that feeding a HF-LS diet to dogs does not affect bacterial diversity or fermentative end products in feces, but may have a negative impact on Prevotella and xylan fermentation.					
30060077	16	30	theme	180-min	2259:2265	arg1	postfeeding					2267:2277	180-min postfeeding	2259:2277	180-min postfeeding	2259:2277	Blood LPS did not increase at 180-min postfeeding with either diet (P < 0.10).					
30060077	1	31	from	microbiota	140:149	arg1	rodents					154:160	rodents	154:160	rodents	154:160	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	7	32	theme	[by	1235:1237	arg1	sequencing					1267:1276	composition [by Ion-Torrent next-generation sequencing	1223:1276	composition [by Ion-Torrent next-generation sequencing	1223:1276	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	11	33	theme	bile	1721:1724	arg1	concentration					1731:1743	a greater (P < 0.05) fecal bile acid concentration	1694:1743	a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet	1694:1772	Feeding the HF-LS diet led to a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet.					
30060077	3	34	dep	HF-LS	659:663	arg1	ME					698:699	25% ME	694:699	25% ME	694:699	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	3	34	dep	HF-LS	659:663	arg1	%					682:682	approximately 43%	666:682	approximately 43%	666:682	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	3	34	dep	HF-LS	659:663	arg1	%					687:687	22%	685:687	22%	685:687	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	1	35	theme	hindgut	132:138	arg1	microbiota					140:149	the hindgut microbiota	128:149	the hindgut microbiota in rodents	128:160	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	2	36	theme	microbiota	438:447	arg1	activity					413:420	activity	413:420	activity	413:420	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	2	36	theme	microbiota	438:447	arg1	composition					397:407	composition	397:407	composition	397:407	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	0	37	from	Effect	0:5	arg1	composition					60:70	fecal microbiota composition	43:70	fecal microbiota composition	43:70	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.					
30060077	0	37	from	Effect	0:5	arg1	activity					76:83	activity	76:83	activity	76:83	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.					
30060077	4	38	theme	broken	947:952	arg1	rice					954:957	broken rice	947:957	broken rice	947:957	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	6	39	theme	daily	1051:1055	arg1	requirements					1064:1075	their daily energy requirements	1045:1075	their daily energy requirements (set at 480 kJ ME/kg BW0.75)	1045:1104	Dogs were fed to meet their daily energy requirements (set at 480 kJ ME/kg BW0.75).					
30060077	7	40	theme	diversity	1208:1216	arg1	analysis					1176:1183	the analysis	1172:1183	the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA	1172:1307	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	2	41	theme	canine	425:430	arg1	microbiota					438:447	canine fecal microbiota	425:447	canine fecal microbiota	425:447	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	2	42	theme	diets	327:331	arg1	feeding					316:322	feeding	316:322	feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota	316:447	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	6	43	dep	requirements	1064:1075	arg1	set					1078:1080	set	1078:1080	set at 480 kJ ME/kg BW0.75	1078:1103	Dogs were fed to meet their daily energy requirements (set at 480 kJ ME/kg BW0.75).					
30060077	14	44	theme	ammonia	2012:2018	arg1	contents					2028:2035	Fecal ammonia and VFA contents	2006:2035	Fecal ammonia and VFA contents	2006:2035	Fecal ammonia and VFA contents were not affected by diet (P > 0.10).					
30060077	2	45	theme	feeding	316:322	arg1	effect					306:311	the effect	302:311	the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota	302:447	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	15	46	theme	xylan	2128:2132	arg1	fermentation					2112:2123	in vitro fermentation	2103:2123	in vitro fermentation of xylan using feces of dogs fed the HF-LS diet	2103:2171	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	1	47	theme	diets	225:229	arg1	impact					206:211	the impact	202:211	the impact of high-fat diets on canine gut microbiota	202:254	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	0	48	from	activity	76:83	arg1	dogs1					88:92	dogs1	88:92	dogs1	88:92	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.					
30060077	13	49	theme	Prevotella	1900:1909	arg1	abundance					1887:1895	a lower relative abundance	1870:1895	a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05)	1870:1976	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	2	50	theme	ME	360:361	arg1	ME					360:361	ME	360:361	ME provided by fat and starch on the composition and activity of canine fecal microbiota	360:447	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	2	50	theme	ME	360:361	arg1	amount					350:355	the amount	346:355	the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota	346:447	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	5	51	theme	diet	1006:1009	arg1	adaptation					1011:1020	diet adaptation	1006:1020	diet adaptation	1006:1020	Each period lasted 7 wk and included 4 wk for diet adaptation.					
30060077	0	52	theme	dietary	10:16	arg1	fat					18:20	dietary fat	10:20	dietary fat	10:20	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.					
30060077	1	53	theme	canine	234:239	arg1	microbiota					245:254	canine gut microbiota	234:254	canine gut microbiota	234:254	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	7	54	theme	bacterial	1188:1196	arg1	richness					1198:1205	bacterial richness	1188:1205	bacterial richness	1188:1205	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	13	55	theme	P	1912:1912	arg1	Prevotella					1900:1909	Prevotella	1900:1909	Prevotella (P < 0.01)	1900:1920	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	13	55	theme	P	1912:1912	arg1	<					1914:1914	P < 0.01	1912:1919	P < 0.01	1912:1919	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	6	56	theme	ME/kg	1092:1096	arg1	BW0.75					1098:1103	480 kJ ME/kg BW0.75	1085:1103	480 kJ ME/kg BW0.75	1085:1103	Dogs were fed to meet their daily energy requirements (set at 480 kJ ME/kg BW0.75).					
30060077	15	57	theme	in	2103:2104	arg1	fermentation					2112:2123	in vitro fermentation	2103:2123	in vitro fermentation of xylan using feces of dogs fed the HF-LS diet	2103:2171	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	13	58	theme	lower	1872:1876	arg1	abundance					1887:1895	a lower relative abundance	1870:1895	a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05)	1870:1976	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	9	59	theme	Gas	1467:1469	arg1	production					1471:1480	Gas production	1467:1480	Gas production	1467:1480	Gas production was measured at 2, 4, 6, 9, 12, and 24 h of incubation.					
30060077	16	60	theme	P	2297:2297	arg1	diet					2291:2294	either diet	2284:2294	either diet (P < 0.10)	2284:2305	Blood LPS did not increase at 180-min postfeeding with either diet (P < 0.10).					
30060077	16	60	theme	P	2297:2297	arg1	<					2299:2299	P < 0.10	2297:2304	P < 0.10	2297:2304	Blood LPS did not increase at 180-min postfeeding with either diet (P < 0.10).					
30060077	16	61	theme	Blood	2229:2233	arg1	LPS					2235:2237	Blood LPS	2229:2237	Blood LPS	2229:2237	Blood LPS did not increase at 180-min postfeeding with either diet (P < 0.10).					
30060077	17	62	theme	bacterial	2382:2390	arg1	diversity					2392:2400	bacterial diversity	2382:2400	bacterial diversity	2382:2400	These findings indicate that feeding a HF-LS diet to dogs does not affect bacterial diversity or fermentative end products in feces, but may have a negative impact on Prevotella and xylan fermentation.					
30060077	4	63	theme	HF-LS	824:828	arg1	diet					830:833	the HF-LS diet	820:833	the HF-LS diet	820:833	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	17	64	theme	fermentative	2405:2416	arg1	products					2422:2429	fermentative end products	2405:2429	fermentative end products	2405:2429	These findings indicate that feeding a HF-LS diet to dogs does not affect bacterial diversity or fermentative end products in feces, but may have a negative impact on Prevotella and xylan fermentation.					
30060077	17	65	from	products	2422:2429	arg1	feces					2434:2438	feces	2434:2438	feces	2434:2438	These findings indicate that feeding a HF-LS diet to dogs does not affect bacterial diversity or fermentative end products in feces, but may have a negative impact on Prevotella and xylan fermentation.					
30060077	13	66	theme	HF-LS	1994:1998	arg1	diet					2000:2003	the HF-LS diet	1990:2003	the HF-LS diet	1990:2003	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	14	67	theme	Fecal	2006:2010	arg1	contents					2028:2035	Fecal ammonia and VFA contents	2006:2035	Fecal ammonia and VFA contents	2006:2035	Fecal ammonia and VFA contents were not affected by diet (P > 0.10).					
30060077	8	68	theme	fecal	1321:1325	arg1	samples					1327:1333	Additional fecal samples	1310:1333	Additional fecal samples	1310:1333	Additional fecal samples were collected from four dogs per diet and period to use as inocula for in vitro fermentation using xylan and pectin as substrates.					
30060077	3	69	theme	2-period	760:767	arg1	arrangement					779:789	a 2-period crossover arrangement	758:789	a 2-period crossover arrangement	758:789	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	14	70	theme	P	2064:2064	arg1	diet					2058:2061	diet	2058:2061	diet (P > 0.10)	2058:2072	Fecal ammonia and VFA contents were not affected by diet (P > 0.10).					
30060077	14	70	theme	P	2064:2064	arg1	>					2066:2066	P > 0.10	2064:2071	P > 0.10	2064:2071	Fecal ammonia and VFA contents were not affected by diet (P > 0.10).					
30060077	10	71	theme	lipopolysaccharide	1638:1655	arg1	analysis					1616:1623	the analysis	1612:1623	the analysis of bacterial lipopolysaccharide (LPS)	1612:1661	On week 7, blood samples were collected at 0- and 180-min postfeeding for the analysis of bacterial lipopolysaccharide (LPS).					
30060077	17	72	theme	HF-LS	2347:2351	arg1	diet					2353:2356	a HF-LS diet	2345:2356	a HF-LS diet to dogs	2345:2364	These findings indicate that feeding a HF-LS diet to dogs does not affect bacterial diversity or fermentative end products in feces, but may have a negative impact on Prevotella and xylan fermentation.					
30060077	3	73	theme	adult	457:461	arg1	dogs					496:499	Twelve adult (3 to 7 yr of age) spayed Beagle dogs	450:499	Twelve adult (3 to 7 yr of age) spayed Beagle dogs	450:499	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	15	74	theme	P	2218:2218	arg1	h					2215:2215	9 h	2213:2215	9 h (P < 0.05)	2213:2226	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	15	74	theme	P	2218:2218	arg1	<					2220:2220	P < 0.05	2218:2225	P < 0.05	2218:2225	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	13	75	theme	Coprobacillus	1953:1965	arg1	abundance					1887:1895	a lower relative abundance	1870:1895	a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05)	1870:1976	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	4	76	from	amount	876:881	arg1	diet					906:909	the LF-HS diet	896:909	the LF-HS diet	896:909	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	7	77	theme	ammonia	1292:1298	arg1	analysis					1176:1183	the analysis	1172:1183	the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA	1172:1307	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	7	78	theme	acids	1285:1289	arg1	analysis					1176:1183	the analysis	1172:1183	the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA	1172:1307	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	4	79	from	amount	803:808	arg1	diet					830:833	the HF-LS diet	820:833	the HF-LS diet	820:833	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	11	80	theme	LF-HS	1763:1767	arg1	diet					1769:1772	the LF-HS diet	1759:1772	the LF-HS diet	1759:1772	Feeding the HF-LS diet led to a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet.					
30060077	1	81	from	evidence	190:197	arg1	impact					206:211	the impact	202:211	the impact of high-fat diets on canine gut microbiota	202:254	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	1	82	theme	Dietary	95:101	arg1	fat					103:105	Dietary fat	95:105	Dietary fat	95:105	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	7	83	theme	sequencing	1267:1276	arg1	analysis					1176:1183	the analysis	1172:1183	the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA	1172:1307	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	13	84	theme	P	1938:1938	arg1	Solobacterium					1923:1935	Solobacterium	1923:1935	Solobacterium (P < 0.05)	1923:1946	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	13	84	theme	P	1938:1938	arg1	<					1940:1940	P < 0.05	1938:1945	P < 0.05	1938:1945	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	4	85	dep	provided	839:846	arg1	whereas					857:863	whereas	857:863	whereas	857:863	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	7	86	theme	Ion-Torrent	1239:1249	arg1	sequencing					1267:1276	composition [by Ion-Torrent next-generation sequencing	1223:1276	composition [by Ion-Torrent next-generation sequencing	1223:1276	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	12	87	theme	P	1838:1838	arg1	diets					1831:1835	diets	1831:1835	diets (P > 0.10)	1831:1846	Bacterial richness and diversity did not differ between diets (P > 0.10).					
30060077	12	87	theme	P	1838:1838	arg1	>					1840:1840	P > 0.10	1838:1845	P > 0.10	1838:1845	Bacterial richness and diversity did not differ between diets (P > 0.10).					
30060077	4	88	theme	starch	886:891	arg1	starch					886:891	starch	886:891	starch	886:891	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	4	88	theme	starch	886:891	arg1	amount					876:881	the higher amount	865:881	the higher amount of starch in the LF-HS diet	865:909	The higher amount of fat in the HF-LS diet was provided by lard, whereas the higher amount of starch in the LF-HS diet was provided primarily by maize and broken rice.					
30060077	0	89	theme	microbiota	49:58	arg1	composition					60:70	fecal microbiota composition	43:70	fecal microbiota composition	43:70	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.					
30060077	3	90	theme	Beagle	489:494	arg1	dogs					496:499	Twelve adult (3 to 7 yr of age) spayed Beagle dogs	450:499	Twelve adult (3 to 7 yr of age) spayed Beagle dogs	450:499	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	8	91	theme	in	1407:1408	arg1	fermentation					1416:1427	in vitro fermentation	1407:1427	in vitro fermentation using xylan	1407:1439	Additional fecal samples were collected from four dogs per diet and period to use as inocula for in vitro fermentation using xylan and pectin as substrates.					
30060077	7	92	theme	composition	1223:1233	arg1	sequencing					1267:1276	composition [by Ion-Torrent next-generation sequencing	1223:1276	composition [by Ion-Torrent next-generation sequencing	1223:1276	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	17	93	from	diversity	2392:2400	arg1	feces					2434:2438	feces	2434:2438	feces	2434:2438	These findings indicate that feeding a HF-LS diet to dogs does not affect bacterial diversity or fermentative end products in feces, but may have a negative impact on Prevotella and xylan fermentation.					
30060077	3	94	dep	7	469:469	arg1	to					466:467	to	466:467	to	466:467	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	11	95	theme	acid	1726:1729	arg1	concentration					1731:1743	a greater (P < 0.05) fecal bile acid concentration	1694:1743	a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet	1694:1772	Feeding the HF-LS diet led to a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet.					
30060077	11	96	dep	greater	1696:1702	arg1	<					1707:1707	P < 0.05	1705:1712	P < 0.05	1705:1712	Feeding the HF-LS diet led to a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet.					
30060077	10	97	theme	180-min	1588:1594	arg1	postfeeding					1596:1606	0- and 180-min postfeeding	1581:1606	0- and 180-min postfeeding	1581:1606	On week 7, blood samples were collected at 0- and 180-min postfeeding for the analysis of bacterial lipopolysaccharide (LPS).					
30060077	6	98	theme	energy	1057:1062	arg1	requirements					1064:1075	their daily energy requirements	1045:1075	their daily energy requirements (set at 480 kJ ME/kg BW0.75)	1045:1104	Dogs were fed to meet their daily energy requirements (set at 480 kJ ME/kg BW0.75).					
30060077	2	99	theme	fecal	432:436	arg1	microbiota					438:447	canine fecal microbiota	425:447	canine fecal microbiota	425:447	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	12	100	theme	Bacterial	1775:1783	arg1	richness					1785:1792	Bacterial richness	1775:1792	Bacterial richness	1775:1792	Bacterial richness and diversity did not differ between diets (P > 0.10).					
30060077	3	101	theme	high-fat-low-starch	633:651	arg1	diet					653:656	a high-fat-low-starch diet	631:656	a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively)	631:746	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	3	102	theme	%	696:696	arg1	ME					698:699	25% ME	694:699	25% ME	694:699	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	10	103	theme	0-	1581:1582	arg1	postfeeding					1596:1606	0- and 180-min postfeeding	1581:1606	0- and 180-min postfeeding	1581:1606	On week 7, blood samples were collected at 0- and 180-min postfeeding for the analysis of bacterial lipopolysaccharide (LPS).					
30060077	1	104	theme	clear	184:188	arg1	evidence					190:197	no clear evidence	181:197	no clear evidence on the impact of high-fat diets on canine gut microbiota	181:254	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	11	105	theme	P	1705:1705	arg1	<					1707:1707	P < 0.05	1705:1712	P < 0.05	1705:1712	Feeding the HF-LS diet led to a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet.					
30060077	15	106	dep	in	2103:2104	arg1	vitro					2106:2110	vitro	2106:2110	vitro	2106:2110	Relative to the LF-HS diet, in vitro fermentation of xylan using feces of dogs fed the HF-LS diet produced less gas at 6 h (P < 0.01) and 9 h (P < 0.05).					
30060077	0	107	theme	fat	18:20	arg1	Effect					0:5	Effect	0:5	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.	0:93	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.					
30060077	11	108	theme	greater	1696:1702	arg1	concentration					1731:1743	a greater (P < 0.05) fecal bile acid concentration	1694:1743	a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet	1694:1772	Feeding the HF-LS diet led to a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet.					
30060077	0	109	theme	starch	25:30	arg1	content					32:38	starch content	25:38	starch content	25:38	Effect of dietary fat to starch content on fecal microbiota composition and activity in dogs1.					
30060077	1	110	theme	gut	241:243	arg1	microbiota					245:254	canine gut microbiota	234:254	canine gut microbiota	234:254	Dietary fat is known to modulate the hindgut microbiota in rodents; however, there is no clear evidence on the impact of high-fat diets on canine gut microbiota.					
30060077	7	111	theme	richness	1198:1205	arg1	analysis					1176:1183	the analysis	1172:1183	the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA	1172:1307	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	7	112	theme	VFA	1305:1307	arg1	analysis					1176:1183	the analysis	1172:1183	the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA	1172:1307	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	11	113	theme	fecal	1715:1719	arg1	concentration					1731:1743	a greater (P < 0.05) fecal bile acid concentration	1694:1743	a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet	1694:1772	Feeding the HF-LS diet led to a greater (P < 0.05) fecal bile acid concentration compared with the LF-HS diet.					
30060077	3	114	theme	low-fat-high-starch	512:530	arg1	diet					532:535	a low-fat-high-starch diet	510:535	a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively)	510:625	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	6	115	theme	kJ	1089:1090	arg1	BW0.75					1098:1103	480 kJ ME/kg BW0.75	1085:1103	480 kJ ME/kg BW0.75	1085:1103	Dogs were fed to meet their daily energy requirements (set at 480 kJ ME/kg BW0.75).					
30060077	2	116	dep	composition	397:407	arg1	the					393:395	the	393:395	the	393:395	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	13	117	theme	relative	1878:1885	arg1	abundance					1887:1895	a lower relative abundance	1870:1895	a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05)	1870:1976	However, dogs showed a lower relative abundance of Prevotella (P < 0.01), Solobacterium (P < 0.05), and Coprobacillus (P ˂ 0.05) when fed of the HF-LS diet.					
30060077	2	118	theme	study	277:281	arg1	purpose					261:267	The purpose	257:267	The purpose of this study	257:281	The purpose of this study was to investigate the effect of feeding of diets differing in the amount of ME provided by fat and starch on the composition and activity of canine fecal microbiota.					
30060077	17	119	theme	negative	2456:2463	arg1	impact					2465:2470	a negative impact	2454:2470	a negative impact	2454:2470	These findings indicate that feeding a HF-LS diet to dogs does not affect bacterial diversity or fermentative end products in feces, but may have a negative impact on Prevotella and xylan fermentation.					
30060077	3	120	theme	spayed	482:487	arg1	dogs					496:499	Twelve adult (3 to 7 yr of age) spayed Beagle dogs	450:499	Twelve adult (3 to 7 yr of age) spayed Beagle dogs	450:499	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	17	121	theme	end	2418:2420	arg1	products					2422:2429	fermentative end products	2405:2429	fermentative end products	2405:2429	These findings indicate that feeding a HF-LS diet to dogs does not affect bacterial diversity or fermentative end products in feces, but may have a negative impact on Prevotella and xylan fermentation.					
30060077	8	122	theme	Additional	1310:1319	arg1	samples					1327:1333	Additional fecal samples	1310:1333	Additional fecal samples	1310:1333	Additional fecal samples were collected from four dogs per diet and period to use as inocula for in vitro fermentation using xylan and pectin as substrates.					
30060077	3	123	dep	diet	532:535	arg1	LF-HS					538:542	LF-HS	538:542	LF-HS	538:542	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	7	124	theme	period	1161:1166	arg1	weeks					1139:1143	weeks 5 and 6	1139:1151	weeks	1139:1143	Fecal samples were collected on weeks 5 and 6 of each period for the analysis of bacterial richness, diversity, and composition [by Ion-Torrent next-generation sequencing], bile acids, ammonia, and VFA.					
30060077	8	125	dep	in	1407:1408	arg1	vitro					1410:1414	vitro	1410:1414	vitro	1410:1414	Additional fecal samples were collected from four dogs per diet and period to use as inocula for in vitro fermentation using xylan and pectin as substrates.					
30060077	3	126	dep	LF-HS	538:542	arg1	%					566:566	42%	564:566	42%	564:566	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	3	126	dep	LF-HS	538:542	arg1	ME					577:578	25% ME	573:578	25% ME	573:578	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30060077	3	126	dep	LF-HS	538:542	arg1	%					561:561	approximately 23%	545:561	approximately 23%	545:561	Twelve adult (3 to 7 yr of age) spayed Beagle dogs received a low-fat-high-starch diet (LF-HS; approximately 23%, 42%, and 25% ME provided by fat, starch, and CP, respectively) and a high-fat-low-starch diet (HF-LS; approximately 43%, 22%, and 25% ME provided by fat, starch, and CP, respectively) following a 2-period crossover arrangement.					
30195117	1	0	theme	weight	173:178	arg1	UHMWPE					194:199	UHMWPE	194:199	UHMWPE	194:199	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	1	0	theme	weight	173:178	arg1	polyethylene					180:191	The ultra-high molecular weight polyethylene	148:191	The ultra-high molecular weight polyethylene (UHMWPE)	148:200	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	2	1	theme	ethylene	488:495	arg1	dots/poly					477:485	carbon dots/poly	470:485	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	2	1	theme	ethylene	488:495	arg1	glycol					497:502	ethylene glycol	488:502	ethylene glycol	488:502	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	3	2	theme	slow-release	700:711	arg1	properties					713:722	slow-release properties	700:722	slow-release properties	700:722	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	4	3	theme	H&E	854:856	arg1	staining					858:865	H&E staining	854:865	H&E staining	854:865	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	2	4	theme	lubricant	344:352	arg1	release					354:360	lubricant release	344:360	lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties	344:657	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	0	5	theme	synovium	103:110	arg1	tissue					112:117	artificial synovium tissue	92:117	artificial synovium tissue with slow-release lubricant	92:145	Carbon dots intensified poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel as artificial synovium tissue with slow-release lubricant.					
30195117	1	6	theme	short	264:268	arg1	life					278:281	short service life	264:281	short service life	264:281	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	0	7	theme	artificial	92:101	arg1	tissue					112:117	artificial synovium tissue	92:117	artificial synovium tissue with slow-release lubricant	92:145	Carbon dots intensified poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel as artificial synovium tissue with slow-release lubricant.					
30195117	1	8	theme	service	270:276	arg1	life					278:281	short service life	264:281	short service life	264:281	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	3	9	theme	excellent	780:788	arg1	effect					802:807	excellent lubrication effect	780:807	excellent lubrication effect	780:807	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	4	10	from	staining	844:851	arg1	mice					907:910	BALC/c mice	900:910	BALC/c mice	900:910	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	4	11	theme	LIVE/DEAD	834:842	arg1	staining					844:851	LIVE/DEAD staining	834:851	LIVE/DEAD staining	834:851	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	3	12	contain	had	696:698	arg1	hydrogel					687:694	the composite hydrogel	673:694	the composite hydrogel	673:694	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	3	12	contain	had	696:698	arg2	properties					713:722	slow-release properties	700:722	slow-release properties	700:722	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	2	13	theme	glycerophosphate	521:536	arg1	hydrogel					564:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	2	14	theme	mechanical	637:646	arg1	properties					648:657	the rheological and mechanical properties	617:657	properties	648:657	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	3	15	theme	release	733:739	arg1	solution					741:748	the release solution	729:748	the release solution	729:748	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	0	16	with	tissue	112:117	arg1	lubricant					137:145	lubricant	137:145	lubricant	137:145	Carbon dots intensified poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel as artificial synovium tissue with slow-release lubricant.					
30195117	2	17	theme	lubrication	379:389	arg1	effect					391:396	the lubrication effect	375:396	the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties	375:657	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	0	18	theme	Carbon	0:5	arg1	dots					7:10	Carbon dots	0:10	Carbon dots	0:10	Carbon dots intensified poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel as artificial synovium tissue with slow-release lubricant.					
30195117	2	19	theme	rheological	621:631	arg1	properties					648:657	the rheological and mechanical properties	617:657	properties	648:657	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	0	20	theme	poly	24:27	arg1	hydrogel					80:87	poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel	24:87	poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel	24:87	Carbon dots intensified poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel as artificial synovium tissue with slow-release lubricant.					
30195117	4	21	theme	good	942:945	arg1	biocompatibilility					947:964	good biocompatibilility	942:964	good biocompatibilility	942:964	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	1	22	theme	metal	206:210	arg1	pair					229:232	metal artificial joint pair	206:232	metal artificial joint pair	206:232	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	1	23	theme	artificial	212:221	arg1	pair					229:232	metal artificial joint pair	206:232	metal artificial joint pair	206:232	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	0	24	theme	ethylene	30:37	arg1	poly					24:27	poly	24:27	poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel	24:87	Carbon dots intensified poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel as artificial synovium tissue with slow-release lubricant.					
30195117	0	24	theme	ethylene	30:37	arg1	glycol					39:44	ethylene glycol	30:44	ethylene glycol	30:44	Carbon dots intensified poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel as artificial synovium tissue with slow-release lubricant.					
30195117	0	25	theme	glycerophosphate	63:78	arg1	hydrogel					80:87	poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel	24:87	poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel	24:87	Carbon dots intensified poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel as artificial synovium tissue with slow-release lubricant.					
30195117	2	26	theme	joints.This	412:422	arg1	effect					391:396	the lubrication effect	375:396	the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties	375:657	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	2	27	dep	joints.This	412:422	arg1	used					599:602	used	599:602	were used to intensify the rheological and mechanical properties	594:657	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	2	27	dep	joints.This	412:422	arg1	adopted					430:436	adopted	430:436	adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	430:571	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	1	28	theme	joint	223:227	arg1	pair					229:232	metal artificial joint pair	206:232	metal artificial joint pair	206:232	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	2	29	theme	dots/poly	477:485	arg1	hydrogel					564:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	2	30	used	used	599:602	arg2	dots					589:592	the carbon dots	578:592	the carbon dots	578:592	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	2	31	theme	composite	554:562	arg1	hydrogel					564:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	2	32	theme	injectable	441:450	arg1	method					452:457	an injectable method	438:457	an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	438:571	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	4	33	from	assay	827:831	arg1	mice					907:910	BALC/c mice	900:910	BALC/c mice	900:910	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	2	34	theme	artificial	401:410	arg1	joints.This					412:422	artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties	401:657	artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties	401:657	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	2	35	theme	carbon	470:475	arg1	dots/poly					477:485	carbon dots/poly	470:485	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	2	35	theme	carbon	470:475	arg1	glycol					497:502	ethylene glycol	488:502	ethylene glycol	488:502	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	3	36	theme	lubrication	790:800	arg1	effect					802:807	excellent lubrication effect	780:807	excellent lubrication effect	780:807	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	2	37	theme	CDs/PEG/CS/GP	539:551	arg1	hydrogel					564:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	4	38	theme	BALC/c	900:905	arg1	mice					907:910	BALC/c mice	900:910	BALC/c mice	900:910	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	4	39	from	staining	858:865	arg1	mice					907:910	BALC/c mice	900:910	BALC/c mice	900:910	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	4	40	theme	safety	879:884	arg1	evaluation					886:895	safety evaluation	879:895	safety evaluation	879:895	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	4	41	theme	MTT	823:825	arg1	assay					827:831	the MTT assay	819:831	the MTT assay	819:831	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	2	42	theme	carbon	582:587	arg1	dots					589:592	the carbon dots	578:592	the carbon dots	578:592	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	4	43	contain	had	938:940	arg1	hydrogels					928:936	the hydrogels	924:936	the hydrogels	924:936	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	4	43	contain	had	938:940	arg2	biocompatibilility					947:964	good biocompatibilility	942:964	good biocompatibilility	942:964	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	2	44	theme	hydrogel	320:327	arg1	development					303:313	the development	299:313	the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties	299:657	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	4	45	from	evaluation	886:895	arg1	mice					907:910	BALC/c mice	900:910	BALC/c mice	900:910	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	3	46	contain	contained	750:758	arg2	CDs					760:762	CDs	760:762	CDs	760:762	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	3	46	contain	contained	750:758	arg1	solution					741:748	the release solution	729:748	the release solution	729:748	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	3	46	contain	contained	750:758	arg2	GP					773:774	GP	773:774	GP	773:774	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	3	46	contain	contained	750:758	arg2	PEG					765:767	PEG	765:767	PEG	765:767	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	2	47	theme	/chitosan/sodium	504:519	arg1	hydrogel					564:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel	470:571	Here we report the development of a hydrogel which exhibits lubricant release to intensify the lubrication effect of artificial joints.This study adopted an injectable method to prepare carbon dots/poly (ethylene glycol)/chitosan/sodium glycerophosphate (CDs/PEG/CS/GP) composite hydrogel, and the carbon dots were used to intensify the rheological and mechanical properties.					
30195117	3	48	theme	composite	677:685	arg1	hydrogel					687:694	the composite hydrogel	673:694	the composite hydrogel	673:694	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	4	49	dep	assay	827:831	arg1	results					867:873	results	867:873	results	867:873	At last, the MTT assay, LIVE/DEAD staining, H&E staining results and safety evaluation in BALC/c mice proved that the hydrogels had good biocompatibilility and were safety for application in vivo.					
30195117	1	50	theme	ultra-high	152:161	arg1	UHMWPE					194:199	UHMWPE	194:199	UHMWPE	194:199	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	1	50	theme	ultra-high	152:161	arg1	polyethylene					180:191	The ultra-high molecular weight polyethylene	148:191	The ultra-high molecular weight polyethylene (UHMWPE)	148:200	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	0	51	theme	/chitosan/sodium	46:61	arg1	hydrogel					80:87	poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel	24:87	poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel	24:87	Carbon dots intensified poly (ethylene glycol)/chitosan/sodium glycerophosphate hydrogel as artificial synovium tissue with slow-release lubricant.					
30195117	3	52	dep	CDs	760:762	arg1	has					776:778	has	776:778	has excellent lubrication effect	776:807	In addition, the composite hydrogel had slow-release properties, and the release solution contained CDs, PEG and GP has excellent lubrication effect.					
30195117	1	53	theme	molecular	163:171	arg1	UHMWPE					194:199	UHMWPE	194:199	UHMWPE	194:199	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	1	53	theme	molecular	163:171	arg1	polyethylene					180:191	The ultra-high molecular weight polyethylene	148:191	The ultra-high molecular weight polyethylene (UHMWPE)	148:200	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
30195117	1	54	theme	wear	248:251	arg1	debris					253:258	wear debris	248:258	wear debris	248:258	The ultra-high molecular weight polyethylene (UHMWPE) and metal artificial joint pair is limited by wear debris and short service life.					
29846874	6	0	theme	soil	963:966	arg1	sand					986:989	sand	986:989	sand	986:989	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	6	0	theme	soil	963:966	arg1	variables					968:976	soil variables	963:976	soil variables such as sand	963:989	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	11	1	theme	plant	1851:1855	arg1	biomass					1857:1863	plant biomass and soil organic matter	1851:1887	biomass	1857:1863	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	1	2	theme	segregated	282:291	arg1	types					298:302	spatially segregated soil types	272:302	spatially segregated soil types	272:302	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	11	3	from	rhizosphere	1801:1811	arg1	pattern					1771:1777	the abundance pattern	1757:1777	the abundance pattern of diazotrophs in the rhizosphere	1757:1811	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	3	4	from	changes	560:566	arg1	composition					585:595	community composition	575:595	community composition	575:595	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	3	4	from	changes	560:566	arg1	abundance					601:609	abundance	601:609	abundance	601:609	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	10	5	theme	bulk	1736:1739	arg1	soil					1741:1744	bulk soil	1736:1744	bulk soil	1736:1744	The abundance of diazotrophic communities in rhizosphere samples was more affected by seasonality than those of bulk soil.					
29846874	11	6	theme	soil	1869:1872	arg1	matter					1882:1887	plant biomass and soil organic matter	1851:1887	matter	1882:1887	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	4	7	theme	oxidizers	706:714	arg1	genes					673:677	genes	673:677	genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare	673:798	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	3	8	theme	community	575:583	arg1	composition					585:595	community composition	575:595	community composition	575:595	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	12	9	theme	season-dependent	1956:1971	arg1	regulation					1973:1982	a potential season-dependent regulation	1944:1982	a potential season-dependent regulation of diazotrophs exerted by the plant host	1944:2023	These results suggest a potential season-dependent regulation of diazotrophs exerted by the plant host.					
29846874	3	10	theme	set	616:618	arg1	composition					585:595	community composition	575:595	community composition	575:595	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	3	10	theme	set	616:618	arg1	abundance					601:609	abundance	601:609	abundance	601:609	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	6	11	theme	clay	996:999	arg1	content					1003:1009	clay % content	996:1009	clay % content	996:1009	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	8	12	theme	stronger	1364:1371	arg1	control					1373:1379	a potentially stronger control	1350:1379	a potentially stronger control of plant host	1350:1393	This result indicates a potentially stronger control of plant host (rather than soil) on the abundance of these organisms.					
29846874	11	13	theme	abundance	1761:1769	arg1	pattern					1771:1777	the abundance pattern	1757:1777	the abundance pattern of diazotrophs in the rhizosphere	1757:1811	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	1	14	theme	natural	229:235	arg1	systems					237:243	natural systems	229:243	natural systems	229:243	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	11	15	theme	organic	1874:1880	arg1	matter					1882:1887	plant biomass and soil organic matter	1851:1887	matter	1882:1887	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	13	16	theme	host	2127:2130	arg1	plants					2132:2137	host plants	2127:2137	host plants	2127:2137	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	3	17	theme	nitrogen-cycling	623:638	arg1	genes					640:644	nitrogen-cycling genes	623:644	nitrogen-cycling genes	623:644	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	4	18	theme	plant	769:773	arg1	species					775:781	plant species	769:781	the plant species Limonium vulgare	765:798	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	8	19	dep	soil	1408:1411	arg1	rather					1396:1401	rather	1396:1401	rather	1396:1401	This result indicates a potentially stronger control of plant host (rather than soil) on the abundance of these organisms.					
29846874	0	20	theme	plant-root	65:74	arg1	microbiomes					76:86	plant-root microbiomes	65:86	plant-root microbiomes along a salt marsh chronosequence	65:120	Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence.					
29846874	1	21	theme	soil	164:167	arg1	properties					169:178	soil properties	164:178	soil properties	164:178	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	4	22	theme	rhizosphere	745:755	arg1	soil					757:760	rhizosphere soil	745:760	rhizosphere soil	745:760	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	13	23	theme	better	2063:2068	arg1	understanding					2070:2082	a better understanding	2061:2082	a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats	2061:2256	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	0	24	from	Compositional	0:12	arg1	microbiomes					76:86	plant-root microbiomes	65:86	plant-root microbiomes along a salt marsh chronosequence	65:120	Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence.					
29846874	6	25	from	abundance	1042:1050	arg1	soil					1083:1086	the bulk soil	1074:1086	the bulk soil	1074:1086	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	0	26	theme	salt	96:99	arg1	chronosequence					107:120	a salt marsh chronosequence	94:120	a salt marsh chronosequence	94:120	Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence.					
29846874	3	27	theme	salt	470:473	arg1	chronosequence					481:494	an undisturbed salt marsh chronosequence	455:494	an undisturbed salt marsh chronosequence spanning over a century of ecosystem development	455:543	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	0	28	theme	nitrogen-cycling	39:54	arg1	genes					56:60	nitrogen-cycling genes	39:60	nitrogen-cycling genes	39:60	Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence.					
29846874	4	29	theme	Limonium	783:790	arg1	vulgare					792:798	the plant species Limonium vulgare	765:798	the plant species Limonium vulgare	765:798	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	2	30	theme	microbial	418:426	arg1	communities					428:438	microbial communities	418:438	microbial communities	418:438	In addition, distant locations may also lead to biogeographical patterns of microbial communities.					
29846874	8	31	theme	plant	1384:1388	arg1	host					1390:1393	plant host	1384:1393	plant host	1384:1393	This result indicates a potentially stronger control of plant host (rather than soil) on the abundance of these organisms.					
29846874	0	32	theme	abundance	18:26	arg1	changes					28:34	abundance changes	18:34	abundance changes	18:34	Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence.					
29846874	1	33	theme	historical	321:330	arg1	legacies					332:339	distinct historical legacies	312:339	distinct historical legacies	312:339	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	9	34	theme	communities	1549:1559	arg1	composition					1522:1532	the composition	1518:1532	the composition of AOA and AOB communities	1518:1559	Interestingly, the plant host did not have a significant effect on the composition of AOA and AOB communities, being mostly divergent according to soil successional stages.					
29846874	6	35	theme	genes	1065:1069	arg1	abundance					1042:1050	the abundance	1038:1050	the abundance of N-cycling genes in the bulk soil	1038:1086	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	10	36	theme	diazotrophic	1641:1652	arg1	communities					1654:1664	diazotrophic communities	1641:1664	diazotrophic communities	1641:1664	The abundance of diazotrophic communities in rhizosphere samples was more affected by seasonality than those of bulk soil.					
29846874	7	37	theme	organisms	1161:1169	arg1	constant					1241:1248	constant	1241:1248	constant	1241:1248	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	7	37	theme	organisms	1161:1169	arg1	abundance					1130:1138	the abundance	1126:1138	the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively)	1126:1224	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	5	38	theme	distinct	836:843	arg1	stages					858:863	five distinct successional stages	831:863	five distinct successional stages of the chronosequence (ranging from 5 to 105 years)	831:915	Samples were collected across five distinct successional stages of the chronosequence (ranging from 5 to 105 years) at two time-points.					
29846874	7	39	from	samples	1117:1123	arg1	constant					1241:1248	constant	1241:1248	constant	1241:1248	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	7	39	from	samples	1117:1123	arg1	abundance					1130:1138	the abundance	1126:1138	the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively)	1126:1224	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	3	40	theme	ecosystem	523:531	arg1	development					533:543	ecosystem development	523:543	ecosystem development	523:543	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	0	41	theme	genes	56:60	arg1	Compositional					0:12	Compositional	0:12	Compositional	0:12	Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence.					
29846874	0	41	theme	genes	56:60	arg1	changes					28:34	abundance changes	18:34	abundance changes	18:34	Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence.					
29846874	13	42	theme	nitrogen-cycling	2192:2207	arg1	genes					2209:2213	nitrogen-cycling genes	2192:2213	nitrogen-cycling genes	2192:2213	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	10	43	from	abundance	1628:1636	arg1	samples					1681:1687	rhizosphere samples	1669:1687	rhizosphere samples	1669:1687	The abundance of diazotrophic communities in rhizosphere samples was more affected by seasonality than those of bulk soil.					
29846874	7	44	theme	successional	1269:1280	arg1	stages					1282:1287	the successional stages	1265:1287	the successional stages	1265:1287	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	11	45	theme	biomass	1857:1863	arg1	increase					1839:1846	the systematic increase	1824:1846	the systematic increase of plant biomass and soil organic matter along the successional gradient	1824:1919	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	12	46	theme	plant	2014:2018	arg1	host					2020:2023	the plant host	2010:2023	the plant host	2010:2023	These results suggest a potential season-dependent regulation of diazotrophs exerted by the plant host.					
29846874	9	47	theme	successional	1603:1614	arg1	stages					1616:1621	soil successional stages	1598:1621	soil successional stages	1598:1621	Interestingly, the plant host did not have a significant effect on the composition of AOA and AOB communities, being mostly divergent according to soil successional stages.					
29846874	7	48	theme	rhizosphere	1105:1115	arg1	samples					1117:1123	the rhizosphere samples	1101:1123	the rhizosphere samples	1101:1123	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	1	49	theme	soil	293:296	arg1	types					298:302	spatially segregated soil types	272:302	spatially segregated soil types	272:302	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	12	50	theme	diazotrophs	1987:1997	arg1	regulation					1973:1982	a potential season-dependent regulation	1944:1982	a potential season-dependent regulation of diazotrophs exerted by the plant host	1944:2023	These results suggest a potential season-dependent regulation of diazotrophs exerted by the plant host.					
29846874	7	51	from	constant	1241:1248	arg1	samples					1117:1123	the rhizosphere samples	1101:1123	the rhizosphere samples	1101:1123	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	9	52	theme	significant	1496:1506	arg1	effect					1508:1513	a significant effect	1494:1513	a significant effect	1494:1513	Interestingly, the plant host did not have a significant effect on the composition of AOA and AOB communities, being mostly divergent according to soil successional stages.					
29846874	11	53	theme	systematic	1828:1837	arg1	increase					1839:1846	the systematic increase	1824:1846	the systematic increase of plant biomass and soil organic matter along the successional gradient	1824:1919	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	6	54	from	soil	1083:1086	arg1	abundance					1042:1050	the abundance	1038:1050	the abundance of N-cycling genes in the bulk soil	1038:1086	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	1	55	theme	plant-host	184:193	arg1	influences					150:159	the relative influences	137:159	the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems	137:243	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	11	56	from	pattern	1771:1777	arg1	rhizosphere					1801:1811	the rhizosphere	1797:1811	the rhizosphere	1797:1811	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	4	57	theme	ammonia	698:704	arg1	oxidizers					706:714	diazotrophs and ammonia oxidizers	682:714	diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare	682:798	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	1	58	theme	root-associated	198:212	arg1	microbiomes					214:224	root-associated microbiomes	198:224	root-associated microbiomes in natural systems	198:243	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	12	59	theme	potential	1946:1954	arg1	regulation					1973:1982	a potential season-dependent regulation	1944:1982	a potential season-dependent regulation of diazotrophs exerted by the plant host	1944:2023	These results suggest a potential season-dependent regulation of diazotrophs exerted by the plant host.					
29846874	6	60	theme	%	1001:1001	arg1	content					1003:1009	clay % content	996:1009	clay % content	996:1009	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	11	61	from	diazotrophs	1782:1792	arg1	rhizosphere					1801:1811	the rhizosphere	1797:1811	the rhizosphere	1797:1811	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	11	62	theme	diazotrophs	1782:1792	arg1	pattern					1771:1777	the abundance pattern	1757:1777	the abundance pattern of diazotrophs in the rhizosphere	1757:1811	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	6	63	from	genes	1065:1069	arg1	soil					1083:1086	the bulk soil	1074:1086	the bulk soil	1074:1086	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	3	64	theme	genes	640:644	arg1	genes					640:644	nitrogen-cycling genes	623:644	nitrogen-cycling genes	623:644	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	3	64	theme	genes	640:644	arg1	set					616:618	a set	614:618	a set of nitrogen-cycling genes	614:644	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	8	65	dep	indicates	1340:1348	arg1	soil					1408:1411	soil	1408:1411	soil	1408:1411	This result indicates a potentially stronger control of plant host (rather than soil) on the abundance of these organisms.					
29846874	8	66	theme	organisms	1440:1448	arg1	abundance					1421:1429	the abundance	1417:1429	the abundance of these organisms	1417:1448	This result indicates a potentially stronger control of plant host (rather than soil) on the abundance of these organisms.					
29846874	4	67	theme	diazotrophs	682:692	arg1	oxidizers					706:714	diazotrophs and ammonia oxidizers	682:714	diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare	682:798	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	13	68	theme	soil	2118:2121	arg1	formation					2103:2111	the natural formation	2091:2111	the natural formation of a soil and host plants	2091:2137	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	9	69	theme	plant	1470:1474	arg1	host					1476:1479	the plant host	1466:1479	the plant host	1466:1479	Interestingly, the plant host did not have a significant effect on the composition of AOA and AOB communities, being mostly divergent according to soil successional stages.					
29846874	13	70	from	changes	2181:2187	arg1	bulk					2218:2221	bulk	2218:2221	bulk	2218:2221	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	13	70	from	changes	2181:2187	arg1	microhabitats					2244:2256	rhizosphere soil microhabitats	2227:2256	rhizosphere soil microhabitats	2227:2256	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	1	71	theme	relative	141:148	arg1	influences					150:159	the relative influences	137:159	the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems	137:243	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	7	72	theme	seasonal	1308:1315	arg1	variation					1317:1325	seasonal variation	1308:1325	seasonal variation	1308:1325	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	13	73	theme	natural	2095:2101	arg1	formation					2103:2111	the natural formation	2091:2111	the natural formation of a soil and host plants	2091:2137	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	1	74	from	microbiomes	214:224	arg1	systems					237:243	natural systems	229:243	natural systems	229:243	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	13	75	theme	soil	2239:2242	arg1	microhabitats					2244:2256	rhizosphere soil microhabitats	2227:2256	rhizosphere soil microhabitats	2227:2256	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	0	76	from	changes	28:34	arg1	microbiomes					76:86	plant-root microbiomes	65:86	plant-root microbiomes along a salt marsh chronosequence	65:120	Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence.					
29846874	1	77	theme	properties	169:178	arg1	influences					150:159	the relative influences	137:159	the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems	137:243	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	0	78	theme	marsh	101:105	arg1	chronosequence					107:120	a salt marsh chronosequence	94:120	a salt marsh chronosequence	94:120	Compositional and abundance changes of nitrogen-cycling genes in plant-root microbiomes along a salt marsh chronosequence.					
29846874	10	79	theme	rhizosphere	1669:1679	arg1	samples					1681:1687	rhizosphere samples	1669:1687	rhizosphere samples	1669:1687	The abundance of diazotrophic communities in rhizosphere samples was more affected by seasonality than those of bulk soil.					
29846874	4	80	dep	bulk	736:739	arg1	the					732:734	the	732:734	the	732:734	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	2	81	theme	communities	428:438	arg1	patterns					406:413	biogeographical patterns	390:413	biogeographical patterns of microbial communities	390:438	In addition, distant locations may also lead to biogeographical patterns of microbial communities.					
29846874	1	82	from	influences	150:159	arg1	microbiomes					214:224	root-associated microbiomes	198:224	root-associated microbiomes in natural systems	198:243	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	9	83	theme	AOA	1537:1539	arg1	communities					1549:1559	AOA and AOB communities	1537:1559	AOA and AOB communities	1537:1559	Interestingly, the plant host did not have a significant effect on the composition of AOA and AOB communities, being mostly divergent according to soil successional stages.					
29846874	6	84	theme	bulk	1078:1081	arg1	soil					1083:1086	the bulk soil	1074:1086	the bulk soil	1074:1086	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	7	85	dep	organisms	1161:1169	arg1	AOB					1199:1201	AOB	1199:1201	AOB	1199:1201	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	7	85	dep	organisms	1161:1169	arg1	archaea					1190:1196	archaea	1190:1196	archaea	1190:1196	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	7	85	dep	organisms	1161:1169	arg1	bacteria					1177:1184	bacteria	1177:1184	bacteria	1177:1184	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	7	85	dep	organisms	1161:1169	arg1	AOA					1207:1209	AOA	1207:1209	AOA	1207:1209	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	4	86	theme	species	775:781	arg1	vulgare					792:798	the plant species Limonium vulgare	765:798	the plant species Limonium vulgare	765:798	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	3	87	theme	undisturbed	458:468	arg1	chronosequence					481:494	an undisturbed salt marsh chronosequence	455:494	an undisturbed salt marsh chronosequence spanning over a century of ecosystem development	455:543	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	5	88	theme	chronosequence	872:885	arg1	stages					858:863	five distinct successional stages	831:863	five distinct successional stages of the chronosequence (ranging from 5 to 105 years)	831:915	Samples were collected across five distinct successional stages of the chronosequence (ranging from 5 to 105 years) at two time-points.					
29846874	1	89	theme	distinct	312:319	arg1	legacies					332:339	distinct historical legacies	312:339	distinct historical legacies	312:339	Disentangling the relative influences of soil properties and plant-host on root-associated microbiomes in natural systems is challenging, given that spatially segregated soil types display distinct historical legacies.					
29846874	9	90	theme	AOB	1545:1547	arg1	communities					1549:1559	AOA and AOB communities	1537:1559	AOA and AOB communities	1537:1559	Interestingly, the plant host did not have a significant effect on the composition of AOA and AOB communities, being mostly divergent according to soil successional stages.					
29846874	4	91	theme	vulgare	792:798	arg1	bulk					736:739	bulk	736:739	bulk	736:739	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	4	91	theme	vulgare	792:798	arg1	soil					757:760	rhizosphere soil	745:760	rhizosphere soil	745:760	Specifically, we targeted genes of diazotrophs and ammonia oxidizers associated with the bulk and rhizosphere soil of the plant species Limonium vulgare.					
29846874	10	92	theme	communities	1654:1664	arg1	abundance					1628:1636	The abundance	1624:1636	The abundance of diazotrophic communities in rhizosphere samples	1624:1687	The abundance of diazotrophic communities in rhizosphere samples was more affected by seasonality than those of bulk soil.					
29846874	7	93	theme	ammonia-oxidizing	1143:1159	arg1	organisms					1161:1169	ammonia-oxidizing organisms	1143:1169	ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively)	1143:1224	However, in the rhizosphere samples, the abundance of ammonia-oxidizing organisms (both bacteria and archaea, AOB and AOA, respectively) was relatively constant across most of the successional stages, albeit displaying seasonal variation.					
29846874	13	94	theme	rhizosphere	2227:2237	arg1	microhabitats					2244:2256	rhizosphere soil microhabitats	2227:2256	rhizosphere soil microhabitats	2227:2256	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	6	95	theme	N-cycling	1055:1063	arg1	genes					1065:1069	N-cycling genes	1055:1069	N-cycling genes in the bulk soil	1055:1086	Our results indicate that soil variables such as sand:silt:clay % content and pH strongly relates to the abundance of N-cycling genes in the bulk soil.					
29846874	11	96	theme	matter	1882:1887	arg1	increase					1839:1846	the systematic increase	1824:1846	the systematic increase of plant biomass and soil organic matter along the successional gradient	1824:1919	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	8	97	theme	host	1390:1393	arg1	control					1373:1379	a potentially stronger control	1350:1379	a potentially stronger control of plant host	1350:1393	This result indicates a potentially stronger control of plant host (rather than soil) on the abundance of these organisms.					
29846874	11	98	theme	successional	1899:1910	arg1	gradient					1912:1919	the successional gradient	1895:1919	the successional gradient	1895:1919	Moreover, the abundance pattern of diazotrophs in the rhizosphere related to the systematic increase of plant biomass and soil organic matter along the successional gradient.					
29846874	5	99	theme	successional	845:856	arg1	stages					858:863	five distinct successional stages	831:863	five distinct successional stages of the chronosequence (ranging from 5 to 105 years)	831:915	Samples were collected across five distinct successional stages of the chronosequence (ranging from 5 to 105 years) at two time-points.					
29846874	3	100	theme	development	533:543	arg1	century					512:518	a century	510:518	a century of ecosystem development	510:543	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	13	101	theme	genes	2209:2213	arg1	changes					2181:2187	the compositional and abundance changes	2149:2187	changes	2181:2187	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	3	102	used	used	450:453	arg2	we					447:448	we	447:448	we	447:448	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	9	103	contain	have	1489:1492	arg2	effect					1508:1513	a significant effect	1494:1513	a significant effect	1494:1513	Interestingly, the plant host did not have a significant effect on the composition of AOA and AOB communities, being mostly divergent according to soil successional stages.					
29846874	9	103	contain	have	1489:1492	arg1	host					1476:1479	the plant host	1466:1479	the plant host	1466:1479	Interestingly, the plant host did not have a significant effect on the composition of AOA and AOB communities, being mostly divergent according to soil successional stages.					
29846874	2	104	theme	distant	355:361	arg1	locations					363:371	distant locations	355:371	distant locations	355:371	In addition, distant locations may also lead to biogeographical patterns of microbial communities.					
29846874	13	105	theme	compositional	2153:2165	arg1	changes					2181:2187	the compositional and abundance changes	2149:2187	changes	2181:2187	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	9	106	theme	soil	1598:1601	arg1	stages					1616:1621	soil successional stages	1598:1621	soil successional stages	1598:1621	Interestingly, the plant host did not have a significant effect on the composition of AOA and AOB communities, being mostly divergent according to soil successional stages.					
29846874	13	107	theme	plants	2132:2137	arg1	formation					2103:2111	the natural formation	2091:2111	the natural formation of a soil and host plants	2091:2137	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
29846874	3	108	theme	marsh	475:479	arg1	chronosequence					481:494	an undisturbed salt marsh chronosequence	455:494	an undisturbed salt marsh chronosequence spanning over a century of ecosystem development	455:543	Here, we used an undisturbed salt marsh chronosequence spanning over a century of ecosystem development to investigate changes in the community composition and abundance of a set of nitrogen-cycling genes.					
29846874	2	109	theme	biogeographical	390:404	arg1	patterns					406:413	biogeographical patterns	390:413	biogeographical patterns of microbial communities	390:438	In addition, distant locations may also lead to biogeographical patterns of microbial communities.					
29846874	13	110	theme	abundance	2171:2179	arg1	changes					2181:2187	the compositional and abundance changes	2149:2187	changes	2181:2187	Overall, this study contributes to a better understanding of how the natural formation of a soil and host plants influence the compositional and abundance changes of nitrogen-cycling genes in bulk and rhizosphere soil microhabitats.					
30879808	0	0	theme	Acid	77:80	arg1	whey					82:85	Acid whey	77:85	Acid whey	77:85	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30879808	7	1	dep	value-added	1135:1145	arg1	uses					1147:1150	uses	1147:1150	uses	1147:1150	These data will assist processors and researchers in developing value-added uses of these dairy coproducts.					
30879808	1	2	from	cheese	287:292	arg1	whey					234:237	acid whey	229:237	acid whey from Greek yogurt (GAW)	229:261	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	2	from	cheese	287:292	arg1	streams					220:226	3 abundantly available but little characterized dairy coproduct streams	156:226	3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	156:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	2	from	cheese	287:292	arg1	whey					269:272	acid whey	264:272	acid whey from cottage cheese (CAW)	264:298	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	2	from	cheese	287:292	arg1	milk					305:308	milk permeate	305:317	milk permeate (MP)	305:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	2	from	cheese	287:292	arg1	MP					320:321	MP	320:321	MP	320:321	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	3	3	dep	2.1	485:487	arg1	to					477:478	to	477:478	to	477:478	The main component in all streams was lactose, with up to 3.5, 2.1, and 11.9% in GAW, CAW, and MP, respectively.					
30879808	1	4	theme	Greek	244:248	arg1	yogurt					250:255	Greek yogurt	244:255	Greek yogurt (GAW)	244:261	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	4	theme	Greek	244:248	arg1	GAW					258:260	GAW	258:260	GAW	258:260	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	6	5	theme	highest	1031:1037	arg1	content					1047:1053	highest mineral content	1031:1053	highest mineral content of all streams	1031:1068	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	5	6	theme	biochemical	859:869	arg1	demand					878:883	biochemical oxygen demand	859:883	biochemical oxygen demand	859:883	Chemical oxygen demand varied from 52,400 to 62,400 mg/L for GAW, 31,900 to 40,000 mg/L for CAW, and 127000 to 142,000 mg/L for MP; biochemical oxygen demand ranged from 45,800 to 50,500 mg/L (GAW), 32,700 to 40,000 mg/L (CAW), and 110,000 to 182,000 mg/L (MP).					
30879808	5	7	dep	40,000	936:941	arg1	to					933:934	to	933:934	to	933:934	Chemical oxygen demand varied from 52,400 to 62,400 mg/L for GAW, 31,900 to 40,000 mg/L for CAW, and 127000 to 142,000 mg/L for MP; biochemical oxygen demand ranged from 45,800 to 50,500 mg/L (GAW), 32,700 to 40,000 mg/L (CAW), and 110,000 to 182,000 mg/L (MP).					
30879808	5	8	dep	50,500	907:912	arg1	to					904:905	to	904:905	to	904:905	Chemical oxygen demand varied from 52,400 to 62,400 mg/L for GAW, 31,900 to 40,000 mg/L for CAW, and 127000 to 142,000 mg/L for MP; biochemical oxygen demand ranged from 45,800 to 50,500 mg/L (GAW), 32,700 to 40,000 mg/L (CAW), and 110,000 to 182,000 mg/L (MP).					
30879808	4	9	dep	6.37	707:710	arg1	to					704:705	to	704:705	to	704:705	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	5	10	theme	oxygen	871:876	arg1	demand					878:883	biochemical oxygen demand	859:883	biochemical oxygen demand	859:883	Chemical oxygen demand varied from 52,400 to 62,400 mg/L for GAW, 31,900 to 40,000 mg/L for CAW, and 127000 to 142,000 mg/L for MP; biochemical oxygen demand ranged from 45,800 to 50,500 mg/L (GAW), 32,700 to 40,000 mg/L (CAW), and 110,000 to 182,000 mg/L (MP).					
30879808	5	11	theme	oxygen	736:741	arg1	demand					743:748	Chemical oxygen demand	727:748	Chemical oxygen demand	727:748	Chemical oxygen demand varied from 52,400 to 62,400 mg/L for GAW, 31,900 to 40,000 mg/L for CAW, and 127000 to 142,000 mg/L for MP; biochemical oxygen demand ranged from 45,800 to 50,500 mg/L (GAW), 32,700 to 40,000 mg/L (CAW), and 110,000 to 182,000 mg/L (MP).					
30879808	5	12	theme	Chemical	727:734	arg1	demand					743:748	Chemical oxygen demand	727:748	Chemical oxygen demand	727:748	Chemical oxygen demand varied from 52,400 to 62,400 mg/L for GAW, 31,900 to 40,000 mg/L for CAW, and 127000 to 142,000 mg/L for MP; biochemical oxygen demand ranged from 45,800 to 50,500 mg/L (GAW), 32,700 to 40,000 mg/L (CAW), and 110,000 to 182,000 mg/L (MP).					
30879808	3	13	with	lactose	460:466	arg1	%					498:498	up to 3.5, 2.1, and 11.9%	474:498	up to 3.5, 2.1, and 11.9% in GAW, CAW, and MP, respectively	474:532	The main component in all streams was lactose, with up to 3.5, 2.1, and 11.9% in GAW, CAW, and MP, respectively.					
30879808	5	14	dep	62,400	772:777	arg1	to					769:770	to	769:770	to	769:770	Chemical oxygen demand varied from 52,400 to 62,400 mg/L for GAW, 31,900 to 40,000 mg/L for CAW, and 127000 to 142,000 mg/L for MP; biochemical oxygen demand ranged from 45,800 to 50,500 mg/L (GAW), 32,700 to 40,000 mg/L (CAW), and 110,000 to 182,000 mg/L (MP).					
30879808	2	15	from	processors	396:405	arg1	dates					371:375	different dates	361:375	different dates from several dairy processors	361:405	Three replicate samples obtained on different dates from several dairy processors were analyzed.					
30879808	4	16	from	mg/g	608:611	arg1	MP					645:646	MP	645:646	MP	645:646	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	4	16	from	mg/g	608:611	arg1	CAW					616:618	CAW	616:618	CAW	616:618	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	4	17	theme	Crude	535:539	arg1	content					549:555	Crude protein content	535:555	Crude protein content	535:555	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	5	18	dep	40,000	803:808	arg1	to					800:801	to	800:801	to	800:801	Chemical oxygen demand varied from 52,400 to 62,400 mg/L for GAW, 31,900 to 40,000 mg/L for CAW, and 127000 to 142,000 mg/L for MP; biochemical oxygen demand ranged from 45,800 to 50,500 mg/L (GAW), 32,700 to 40,000 mg/L (CAW), and 110,000 to 182,000 mg/L (MP).					
30879808	6	19	theme	streams	1062:1068	arg1	content					1047:1053	highest mineral content	1031:1053	highest mineral content of all streams	1031:1068	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	6	19	theme	streams	1062:1068	arg1	4.21-4.48					1016:1024	4.21-4.48	1016:1024	4.21-4.48	1016:1024	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	6	19	theme	streams	1062:1068	arg1	pH					1012:1013	the lowest pH	1001:1013	the lowest pH (4.21-4.48)	1001:1025	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	0	20	dep	whey	82:85	arg1	permeate					96:103	permeate	96:103	permeate	96:103	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30879808	1	21	theme	available	169:177	arg1	whey					234:237	acid whey	229:237	acid whey from Greek yogurt (GAW)	229:261	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	21	theme	available	169:177	arg1	whey					269:272	acid whey	264:272	acid whey from cottage cheese (CAW)	264:298	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	21	theme	available	169:177	arg1	milk					305:308	milk permeate	305:317	milk permeate (MP)	305:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	21	theme	available	169:177	arg1	streams					220:226	3 abundantly available but little characterized dairy coproduct streams	156:226	3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	156:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	2	22	theme	dairy	390:394	arg1	processors					396:405	several dairy processors	382:405	several dairy processors	382:405	Three replicate samples obtained on different dates from several dairy processors were analyzed.					
30879808	0	23	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.	0:104	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30879808	3	24	from	component	431:439	arg1	streams					448:454	all streams	444:454	all streams	444:454	The main component in all streams was lactose, with up to 3.5, 2.1, and 11.9% in GAW, CAW, and MP, respectively.					
30879808	4	25	theme	protein	541:547	arg1	content					549:555	Crude protein content	535:555	Crude protein content	535:555	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	1	26	dep	characterized	190:202	arg1	little					183:188	little	183:188	little	183:188	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	27	theme	characterized	190:202	arg1	whey					234:237	acid whey	229:237	acid whey from Greek yogurt (GAW)	229:261	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	27	theme	characterized	190:202	arg1	whey					269:272	acid whey	264:272	acid whey from cottage cheese (CAW)	264:298	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	27	theme	characterized	190:202	arg1	milk					305:308	milk permeate	305:317	milk permeate (MP)	305:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	27	theme	characterized	190:202	arg1	streams					220:226	3 abundantly available but little characterized dairy coproduct streams	156:226	3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	156:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	28	theme	acid	264:267	arg1	whey					269:272	acid whey	264:272	acid whey from cottage cheese (CAW)	264:298	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	28	theme	acid	264:267	arg1	streams					220:226	3 abundantly available but little characterized dairy coproduct streams	156:226	3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	156:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	2	29	theme	several	382:388	arg1	processors					396:405	several dairy processors	382:405	several dairy processors	382:405	Three replicate samples obtained on different dates from several dairy processors were analyzed.					
30879808	4	30	dep	3.71	577:580	arg1	to					574:575	to	574:575	to	574:575	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	3	31	from	%	498:498	arg1	CAW					508:510	CAW	508:510	CAW	508:510	The main component in all streams was lactose, with up to 3.5, 2.1, and 11.9% in GAW, CAW, and MP, respectively.					
30879808	3	31	from	%	498:498	arg1	MP					517:518	MP	517:518	MP	517:518	The main component in all streams was lactose, with up to 3.5, 2.1, and 11.9% in GAW, CAW, and MP, respectively.					
30879808	3	31	from	%	498:498	arg1	GAW					503:505	GAW	503:505	GAW	503:505	The main component in all streams was lactose, with up to 3.5, 2.1, and 11.9% in GAW, CAW, and MP, respectively.					
30879808	1	32	theme	dairy	204:208	arg1	whey					234:237	acid whey	229:237	acid whey from Greek yogurt (GAW)	229:261	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	32	theme	dairy	204:208	arg1	whey					269:272	acid whey	264:272	acid whey from cottage cheese (CAW)	264:298	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	32	theme	dairy	204:208	arg1	milk					305:308	milk permeate	305:317	milk permeate (MP)	305:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	32	theme	dairy	204:208	arg1	streams					220:226	3 abundantly available but little characterized dairy coproduct streams	156:226	3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	156:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	0	33	theme	coproduct	36:44	arg1	streams					46:52	coproduct streams	36:52	coproduct streams	36:52	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30879808	1	34	theme	coproduct	210:218	arg1	whey					234:237	acid whey	229:237	acid whey from Greek yogurt (GAW)	229:261	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	34	theme	coproduct	210:218	arg1	whey					269:272	acid whey	264:272	acid whey from cottage cheese (CAW)	264:298	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	34	theme	coproduct	210:218	arg1	milk					305:308	milk permeate	305:317	milk permeate (MP)	305:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	34	theme	coproduct	210:218	arg1	streams					220:226	3 abundantly available but little characterized dairy coproduct streams	156:226	3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	156:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	4	35	dep	4.35	632:635	arg1	to					629:630	to	629:630	to	629:630	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	1	36	from	yogurt	250:255	arg1	whey					234:237	acid whey	229:237	acid whey from Greek yogurt (GAW)	229:261	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	36	from	yogurt	250:255	arg1	streams					220:226	3 abundantly available but little characterized dairy coproduct streams	156:226	3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	156:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	36	from	yogurt	250:255	arg1	whey					269:272	acid whey	264:272	acid whey from cottage cheese (CAW)	264:298	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	36	from	yogurt	250:255	arg1	milk					305:308	milk permeate	305:317	milk permeate (MP)	305:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	36	from	yogurt	250:255	arg1	MP					320:321	MP	320:321	MP	320:321	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	0	37	from	processing	65:74	arg1	Composition					21:31	Composition	21:31	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.	0:104	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30879808	2	38	dep	samples	341:347	arg1	replicate					331:339	replicate	331:339	replicate	331:339	Three replicate samples obtained on different dates from several dairy processors were analyzed.					
30879808	6	39	theme	lowest	1005:1010	arg1	4.21-4.48					1016:1024	4.21-4.48	1016:1024	4.21-4.48	1016:1024	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	6	39	theme	lowest	1005:1010	arg1	pH					1012:1013	the lowest pH	1001:1013	the lowest pH (4.21-4.48)	1001:1025	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	1	40	theme	cottage	279:285	arg1	CAW					295:297	CAW	295:297	CAW	295:297	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	40	theme	cottage	279:285	arg1	cheese					287:292	cottage cheese	279:292	cottage cheese (CAW)	279:298	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	4	41	dep	4.51	690:693	arg1	to					687:688	to	687:688	to	687:688	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	4	42	dep	5.05	603:606	arg1	to					600:601	to	600:601	to	600:601	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	0	43	theme	streams	46:52	arg1	Composition					21:31	Composition	21:31	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.	0:104	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30879808	1	44	dep	milk	305:308	arg1	permeate					310:317	permeate	310:317	permeate	310:317	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	7	45	theme	dairy	1161:1165	arg1	coproducts					1167:1176	these dairy coproducts	1155:1176	these dairy coproducts	1155:1176	These data will assist processors and researchers in developing value-added uses of these dairy coproducts.					
30879808	4	46	from	GAW	590:592	arg1	MP					645:646	MP	645:646	MP	645:646	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	4	46	from	GAW	590:592	arg1	CAW					616:618	CAW	616:618	CAW	616:618	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	5	47	dep	142,000	838:844	arg1	to					835:836	to	835:836	to	835:836	Chemical oxygen demand varied from 52,400 to 62,400 mg/L for GAW, 31,900 to 40,000 mg/L for CAW, and 127000 to 142,000 mg/L for MP; biochemical oxygen demand ranged from 45,800 to 50,500 mg/L (GAW), 32,700 to 40,000 mg/L (CAW), and 110,000 to 182,000 mg/L (MP).					
30879808	7	48	theme	coproducts	1167:1176	arg1	value-added					1135:1145	value-added	1135:1145	value-added	1135:1145	These data will assist processors and researchers in developing value-added uses of these dairy coproducts.					
30879808	4	49	from	mg/g	637:640	arg1	MP					645:646	MP	645:646	MP	645:646	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	4	49	from	mg/g	637:640	arg1	CAW					616:618	CAW	616:618	CAW	616:618	Crude protein content ranged from 1.71 to 3.71 mg/g in GAW, 1.65 to 5.05 mg/g in CAW, and 3.2 to 4.35 mg/g in MP, and pH ranged from 4.21 to 4.48, 4.35 to 4.51, and 5.4 to 6.37, respectively.					
30879808	1	50	dep	streams	220:226	arg1	whey					234:237	acid whey	229:237	acid whey from Greek yogurt (GAW)	229:261	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	50	dep	streams	220:226	arg1	streams					220:226	3 abundantly available but little characterized dairy coproduct streams	156:226	3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	156:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	50	dep	streams	220:226	arg1	whey					269:272	acid whey	264:272	acid whey from cottage cheese (CAW)	264:298	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	50	dep	streams	220:226	arg1	milk					305:308	milk permeate	305:317	milk permeate (MP)	305:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	50	dep	streams	220:226	arg1	MP					320:321	MP	320:321	MP	320:321	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	3	51	theme	main	426:429	arg1	component					431:439	The main component	422:439	The main component in all streams	422:454	The main component in all streams was lactose, with up to 3.5, 2.1, and 11.9% in GAW, CAW, and MP, respectively.					
30879808	3	51	theme	main	426:429	arg1	lactose					460:466	lactose	460:466	lactose	460:466	The main component in all streams was lactose, with up to 3.5, 2.1, and 11.9% in GAW, CAW, and MP, respectively.					
30879808	6	52	contain	had	997:999	arg1	GAW					993:995	The GAW	989:995	The GAW	989:995	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	6	52	contain	had	997:999	arg2	4.21-4.48					1016:1024	4.21-4.48	1016:1024	4.21-4.48	1016:1024	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	6	52	contain	had	997:999	arg2	pH					1012:1013	the lowest pH	1001:1013	the lowest pH (4.21-4.48)	1001:1025	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	6	52	contain	had	997:999	arg2	content					1047:1053	highest mineral content	1031:1053	highest mineral content of all streams	1031:1068	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	1	53	theme	acid	229:232	arg1	whey					234:237	acid whey	229:237	acid whey from Greek yogurt (GAW)	229:261	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	1	53	theme	acid	229:232	arg1	streams					220:226	3 abundantly available but little characterized dairy coproduct streams	156:226	3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	156:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	6	54	theme	mineral	1039:1045	arg1	content					1047:1053	highest mineral content	1031:1053	highest mineral content of all streams	1031:1068	The GAW had the lowest pH (4.21-4.48) and highest mineral content of all streams.					
30879808	0	55	dep	communication	6:18	arg1	Composition					21:31	Composition	21:31	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.	0:104	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30879808	0	55	dep	communication	6:18	arg1	milk					91:94	milk	91:94	milk	91:94	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30879808	0	55	dep	communication	6:18	arg1	whey					82:85	Acid whey	77:85	Acid whey	77:85	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30879808	2	56	theme	different	361:369	arg1	dates					371:375	different dates	361:375	different dates from several dairy processors	361:405	Three replicate samples obtained on different dates from several dairy processors were analyzed.					
30879808	1	57	theme	composition	128:138	arg1	information					140:150	composition information	128:150	composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP)	128:322	This article provides composition information for 3 abundantly available but little characterized dairy coproduct streams: acid whey from Greek yogurt (GAW), acid whey from cottage cheese (CAW), and milk permeate (MP).					
30879808	0	58	theme	dairy	59:63	arg1	processing					65:74	dairy processing	59:74	dairy processing	59:74	Short communication: Composition of coproduct streams from dairy processing: Acid whey and milk permeate.					
30204399	6	0	from	%	959:959	arg1	h					986:986	6 h	984:986	6 h	984:986	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	2	1	from	tissue	406:411	arg1	applications					426:437	practical applications	416:437	practical applications	416:437	Additionally, excellent cell affinity and tissue adhesiveness are also necessary for the hydrogel to integrate with the wound tissue in practical applications.					
30204399	1	2	theme	peculiar	154:161	arg1	properties					163:172	several peculiar properties	146:172	several peculiar properties	146:172	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	4	3	theme	hydrogel	585:592	arg1	ability					570:576	The self-healing ability	553:576	The self-healing ability of the hydrogel	553:592	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	7	4	theme	catechol	1236:1243	arg1	groups					1245:1250	catechol groups	1236:1250	catechol groups	1236:1250	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	6	5	dep	ability	948:954	arg1	%					959:959	80%	957:959	80% mechanical recovery	957:979	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	6	theme	desirable	1075:1083	arg1	properties					1085:1094	highly desirable properties	1068:1094	highly desirable properties	1068:1094	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	6	theme	desirable	1075:1083	arg1	superior					1104:1111	superior	1104:1111	superior	1104:1111	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	6	theme	desirable	1075:1083	arg1	hydrogel					911:918	a novel hydrogel	903:918	a novel hydrogel	903:918	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	7	theme	dressing	1179:1186	arg1	applications					1188:1199	wound dressing applications	1173:1199	wound dressing applications	1173:1199	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	1	8	theme	muscle	256:261	arg1	damage					272:277	muscle and skin damage	256:277	damage	272:277	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	6	9	theme	self-healing	935:946	arg1	ability					948:954	efficient self-healing ability	925:954	efficient self-healing ability (80% mechanical recovery in 6 h)	925:987	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	10	theme	wound	1173:1177	arg1	applications					1188:1199	wound dressing applications	1173:1199	wound dressing applications	1173:1199	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	3	11	theme	cell	502:505	arg1	affinity					507:514	superior cell affinity	493:514	superior cell affinity	493:514	Herein, an ultratough and self-healing hydrogel with superior cell affinity and tissue adhesiveness is prepared.					
30204399	6	12	theme	tensile	995:1001	arg1	strength					1003:1010	high tensile strength	990:1010	high tensile strength (0.109 MPa)	990:1022	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	12	theme	tensile	995:1001	arg1	MPa					1019:1021	0.109 MPa	1013:1021	0.109 MPa	1013:1021	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	13	theme	efficient	925:933	arg1	ability					948:954	efficient self-healing ability	925:954	efficient self-healing ability (80% mechanical recovery in 6 h)	925:987	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	7	14	theme	unique	1291:1296	arg1	affinity					1303:1310	unique cell affinity and tissue adhesiveness	1291:1334	affinity	1303:1310	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	8	15	theme	practical	1366:1374	arg1	utility					1376:1382	the practical utility	1362:1382	the practical utility of our fabricated hydrogel	1362:1409	Moreover, we demonstrate the practical utility of our fabricated hydrogel via both in vivo and in vitro experiments.					
30204399	4	16	theme	oxidized	687:694	arg1	OSA-DA					713:718	OSA-DA	713:718	OSA-DA	713:718	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	4	16	theme	oxidized	687:694	arg1	alginate					703:710	dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains	670:751	alginate	703:710	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	5	17	theme	covalent	758:765	arg1	responsible					784:794	responsible	784:794	responsible	784:794	The covalent cross-linking is responsible for its stable mechanical structure.					
30204399	5	17	theme	covalent	758:765	arg1	cross-linking					767:779	The covalent cross-linking	754:779	The covalent cross-linking	754:779	The covalent cross-linking is responsible for its stable mechanical structure.					
30204399	3	18	with	hydrogel	479:486	arg1	affinity					507:514	superior cell affinity	493:514	superior cell affinity	493:514	Herein, an ultratough and self-healing hydrogel with superior cell affinity and tissue adhesiveness is prepared.					
30204399	3	18	with	hydrogel	479:486	arg1	adhesiveness					527:538	tissue adhesiveness	520:538	tissue adhesiveness	520:538	Herein, an ultratough and self-healing hydrogel with superior cell affinity and tissue adhesiveness is prepared.					
30204399	6	19	theme	tough	1136:1140	arg1	hydrogels					1159:1167	previously reported tough and self-healing hydrogels	1116:1167	previously reported tough and self-healing hydrogels for wound dressing applications	1116:1199	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	4	20	theme	dopamine-grafted	670:685	arg1	OSA-DA					713:718	OSA-DA	713:718	OSA-DA	713:718	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	4	20	theme	dopamine-grafted	670:685	arg1	alginate					703:710	dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains	670:751	alginate	703:710	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	1	21	contain	possess	138:144	arg2	properties					163:172	several peculiar properties	146:172	several peculiar properties	146:172	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	1	21	contain	possess	138:144	arg1	hydrogel					77:84	A hydrogel	75:84	A hydrogel for potential applications in wound dressing	75:129	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	4	22	theme	self-healing	557:568	arg1	ability					570:576	The self-healing ability	553:576	The self-healing ability of the hydrogel	553:592	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	6	23	theme	self-healing	1146:1157	arg1	hydrogels					1159:1167	previously reported tough and self-healing hydrogels	1116:1167	previously reported tough and self-healing hydrogels for wound dressing applications	1116:1199	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	7	24	theme	OSA-DA	1259:1264	arg1	chains					1266:1271	the OSA-DA chains	1255:1271	the OSA-DA chains	1255:1271	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	7	25	theme	cell	1298:1301	arg1	affinity					1303:1310	unique cell affinity and tissue adhesiveness	1291:1334	affinity	1303:1310	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	1	26	theme	skin	267:270	arg1	damage					272:277	muscle and skin damage	256:277	damage	272:277	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	7	27	from	plenty	1226:1231	arg1	chains					1266:1271	the OSA-DA chains	1255:1271	the OSA-DA chains	1255:1271	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	2	28	theme	cell	304:307	arg1	affinity					309:316	excellent cell affinity	294:316	excellent cell affinity	294:316	Additionally, excellent cell affinity and tissue adhesiveness are also necessary for the hydrogel to integrate with the wound tissue in practical applications.					
30204399	1	29	theme	efficient	183:191	arg1	ability					206:212	efficient self-healing ability	183:212	efficient self-healing ability	183:212	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	8	30	theme	in	1432:1433	arg1	experiments					1441:1451	both in vivo and in vitro experiments	1415:1451	both in vivo and in vitro experiments	1415:1451	Moreover, we demonstrate the practical utility of our fabricated hydrogel via both in vivo and in vitro experiments.					
30204399	2	31	theme	excellent	294:302	arg1	affinity					309:316	excellent cell affinity	294:316	excellent cell affinity	294:316	Additionally, excellent cell affinity and tissue adhesiveness are also necessary for the hydrogel to integrate with the wound tissue in practical applications.					
30204399	3	32	theme	ultratough	451:460	arg1	hydrogel					479:486	an ultratough and self-healing hydrogel	448:486	an ultratough and self-healing hydrogel with superior cell affinity and tissue adhesiveness	448:538	Herein, an ultratough and self-healing hydrogel with superior cell affinity and tissue adhesiveness is prepared.					
30204399	2	33	theme	practical	416:424	arg1	applications					426:437	practical applications	416:437	practical applications	416:437	Additionally, excellent cell affinity and tissue adhesiveness are also necessary for the hydrogel to integrate with the wound tissue in practical applications.					
30204399	6	34	theme	novel	905:909	arg1	properties					1085:1094	highly desirable properties	1068:1094	highly desirable properties	1068:1094	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	34	theme	novel	905:909	arg1	superior					1104:1111	superior	1104:1111	superior	1104:1111	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	34	theme	novel	905:909	arg1	hydrogel					911:918	a novel hydrogel	903:918	a novel hydrogel	903:918	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	35	theme	reported	1127:1134	arg1	hydrogels					1159:1167	previously reported tough and self-healing hydrogels	1116:1167	previously reported tough and self-healing hydrogels for wound dressing applications	1116:1199	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	4	36	theme	dynamic	633:639	arg1	cross-linking					648:660	dynamic Schiff cross-linking	633:660	dynamic Schiff cross-linking	633:660	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	0	37	theme	Tissue-Adhesive	30:44	arg1	Hydrogel					46:53	Tissue-Adhesive Hydrogel	30:53	Tissue-Adhesive Hydrogel for Wound Dressing	30:72	Ultratough, Self-Healing, and Tissue-Adhesive Hydrogel for Wound Dressing.					
30204399	3	38	theme	superior	493:500	arg1	affinity					507:514	superior cell affinity	493:514	superior cell affinity	493:514	Herein, an ultratough and self-healing hydrogel with superior cell affinity and tissue adhesiveness is prepared.					
30204399	6	39	with	hydrogel	911:918	arg1	ultrastretchability					1029:1047	ultrastretchability	1029:1047	ultrastretchability (2550%)	1029:1055	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	39	with	hydrogel	911:918	arg1	%					1054:1054	2550%	1050:1054	2550%	1050:1054	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	39	with	hydrogel	911:918	arg1	strength					1003:1010	high tensile strength	990:1010	high tensile strength (0.109 MPa)	990:1022	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	39	with	hydrogel	911:918	arg1	ability					948:954	efficient self-healing ability	925:954	efficient self-healing ability (80% mechanical recovery in 6 h)	925:987	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	39	with	hydrogel	911:918	arg1	MPa					1019:1021	0.109 MPa	1013:1021	0.109 MPa	1013:1021	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	1	40	theme	potential	90:98	arg1	applications					100:111	potential applications	90:111	potential applications in wound dressing	90:129	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	2	41	theme	wound	400:404	arg1	tissue					406:411	the wound tissue	396:411	the wound tissue in practical applications	396:437	Additionally, excellent cell affinity and tissue adhesiveness are also necessary for the hydrogel to integrate with the wound tissue in practical applications.					
30204399	5	42	theme	stable	804:809	arg1	structure					822:830	its stable mechanical structure	800:830	its stable mechanical structure	800:830	The covalent cross-linking is responsible for its stable mechanical structure.					
30204399	6	43	dep	%	959:959	arg1	recovery					972:979	mechanical recovery	961:979	80% mechanical recovery	957:979	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	1	44	theme	mechanical	218:227	arg1	toughness					229:237	mechanical toughness	218:237	mechanical toughness	218:237	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	5	45	theme	mechanical	811:820	arg1	structure					822:830	its stable mechanical structure	800:830	its stable mechanical structure	800:830	The covalent cross-linking is responsible for its stable mechanical structure.					
30204399	8	46	dep	in	1432:1433	arg1	vitro					1435:1439	vitro	1435:1439	vitro	1435:1439	Moreover, we demonstrate the practical utility of our fabricated hydrogel via both in vivo and in vitro experiments.					
30204399	7	47	contain	has	1287:1289	arg2	adhesiveness					1323:1334	unique cell affinity and tissue adhesiveness	1291:1334	adhesiveness	1323:1334	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	7	47	contain	has	1287:1289	arg2	affinity					1303:1310	unique cell affinity and tissue adhesiveness	1291:1334	affinity	1303:1310	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	7	47	contain	has	1287:1289	arg1	hydrogel					1278:1285	the hydrogel	1274:1285	the hydrogel	1274:1285	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	8	48	theme	fabricated	1391:1400	arg1	hydrogel					1402:1409	our fabricated hydrogel	1387:1409	our fabricated hydrogel	1387:1409	Moreover, we demonstrate the practical utility of our fabricated hydrogel via both in vivo and in vitro experiments.					
30204399	8	49	dep	in	1420:1421	arg1	vivo					1423:1426	vivo	1423:1426	vivo	1423:1426	Moreover, we demonstrate the practical utility of our fabricated hydrogel via both in vivo and in vitro experiments.					
30204399	6	50	theme	cross-linking	874:886	arg1	combination					837:847	The combination	833:847	The combination of physical and chemical cross-linking	833:886	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	51	theme	chemical	865:872	arg1	cross-linking					874:886	physical and chemical cross-linking	852:886	physical and chemical cross-linking	852:886	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	1	52	theme	wound	116:120	arg1	dressing					122:129	wound dressing	116:129	wound dressing	116:129	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	6	53	theme	mechanical	961:970	arg1	recovery					972:979	mechanical recovery	961:979	80% mechanical recovery	957:979	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	4	54	theme	sodium	696:701	arg1	OSA-DA					713:718	OSA-DA	713:718	OSA-DA	713:718	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	4	54	theme	sodium	696:701	arg1	alginate					703:710	dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains	670:751	alginate	703:710	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	2	55	theme	tissue	322:327	arg1	adhesiveness					329:340	tissue adhesiveness	322:340	tissue adhesiveness	322:340	Additionally, excellent cell affinity and tissue adhesiveness are also necessary for the hydrogel to integrate with the wound tissue in practical applications.					
30204399	1	56	from	applications	100:111	arg1	dressing					122:129	wound dressing	116:129	wound dressing	116:129	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	4	57	theme	hydrogen	614:621	arg1	bonds					623:627	hydrogen bonds	614:627	hydrogen bonds	614:627	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	8	58	theme	hydrogel	1402:1409	arg1	utility					1376:1382	the practical utility	1362:1382	the practical utility of our fabricated hydrogel	1362:1409	Moreover, we demonstrate the practical utility of our fabricated hydrogel via both in vivo and in vitro experiments.					
30204399	1	59	theme	several	146:152	arg1	properties					163:172	several peculiar properties	146:172	several peculiar properties	146:172	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	3	60	theme	self-healing	466:477	arg1	hydrogel					479:486	an ultratough and self-healing hydrogel	448:486	an ultratough and self-healing hydrogel with superior cell affinity and tissue adhesiveness	448:538	Herein, an ultratough and self-healing hydrogel with superior cell affinity and tissue adhesiveness is prepared.					
30204399	6	61	theme	physical	852:859	arg1	cross-linking					874:886	physical and chemical cross-linking	852:886	physical and chemical cross-linking	852:886	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	8	62	theme	in	1420:1421	arg1	experiments					1441:1451	both in vivo and in vitro experiments	1415:1451	both in vivo and in vitro experiments	1415:1451	Moreover, we demonstrate the practical utility of our fabricated hydrogel via both in vivo and in vitro experiments.					
30204399	1	63	theme	self-healing	193:204	arg1	ability					206:212	efficient self-healing ability	183:212	efficient self-healing ability	183:212	A hydrogel for potential applications in wound dressing should possess several peculiar properties, such as efficient self-healing ability and mechanical toughness, so as to repair muscle and skin damage.					
30204399	7	64	theme	groups	1245:1250	arg1	plenty					1226:1231	plenty	1226:1231	plenty of catechol groups on the OSA-DA chains	1226:1271	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	7	65	theme	tissue	1316:1321	arg1	adhesiveness					1323:1334	unique cell affinity and tissue adhesiveness	1291:1334	adhesiveness	1323:1334	More remarkably, due to plenty of catechol groups on the OSA-DA chains, the hydrogel has unique cell affinity and tissue adhesiveness.					
30204399	0	66	theme	Wound	59:63	arg1	Dressing					65:72	Wound Dressing	59:72	Wound Dressing	59:72	Ultratough, Self-Healing, and Tissue-Adhesive Hydrogel for Wound Dressing.					
30204399	4	67	theme	Schiff	641:646	arg1	cross-linking					648:660	dynamic Schiff cross-linking	633:660	dynamic Schiff cross-linking	633:660	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	6	68	theme	high	990:993	arg1	strength					1003:1010	high tensile strength	990:1010	high tensile strength (0.109 MPa)	990:1022	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	6	68	theme	high	990:993	arg1	MPa					1019:1021	0.109 MPa	1013:1021	0.109 MPa	1013:1021	The combination of physical and chemical cross-linking contributes to a novel hydrogel with efficient self-healing ability (80% mechanical recovery in 6 h), high tensile strength (0.109 MPa), and ultrastretchability (2550%), which are highly desirable properties and are superior to previously reported tough and self-healing hydrogels for wound dressing applications.					
30204399	3	69	theme	tissue	520:525	arg1	adhesiveness					527:538	tissue adhesiveness	520:538	tissue adhesiveness	520:538	Herein, an ultratough and self-healing hydrogel with superior cell affinity and tissue adhesiveness is prepared.					
30204399	4	70	theme	polyacrylamide	725:738	arg1	chains					746:751	dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains	670:751	chains	746:751	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
30204399	4	70	theme	polyacrylamide	725:738	arg1	PAM					741:743	PAM	741:743	PAM	741:743	The self-healing ability of the hydrogel is obtained through hydrogen bonds and dynamic Schiff cross-linking between dopamine-grafted oxidized sodium alginate (OSA-DA) and polyacrylamide (PAM) chains.					
31825967	1	0	from	use	252:254	arg1	livestock					259:267	livestock	259:267	livestock	259:267	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	6	1	theme	Bacteroides	1189:1199	arg1	abundance					1114:1122	higher relative abundance	1098:1122	higher relative abundance of Blautia, Coprococcus and Desulfovibrio	1098:1164	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	6	1	theme	Bacteroides	1189:1199	arg1	abundance					1176:1184	lower abundance	1170:1184	lower abundance of Bacteroides	1170:1199	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	7	2	theme	genetic	1269:1275	arg1	analyses					1288:1295	genetic functional analyses	1269:1295	genetic functional analyses	1269:1295	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	7	3	theme	enrofloxacin	1330:1341	arg1	group					1343:1347	the enrofloxacin group	1326:1347	the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds	1326:1438	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	7	4	theme	higher	1364:1369	arg1	abundance					1380:1388	a higher relative abundance	1362:1388	a higher relative abundance of "Resistance to antibiotics and toxic compounds	1362:1438	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	11	5	theme	minimal	2057:2063	arg1	impacts					2065:2071	minimal impacts	2057:2071	minimal impacts on the microbial composition and genetic functional microbiota of calves	2057:2144	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	8	6	theme	resistance	1614:1623	arg1	"					1624:1624	"Erythromycin resistance"	1600:1624	"Erythromycin resistance"	1600:1624	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	2	7	from	changes	380:386	arg1	function					428:435	function	428:435	function	428:435	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	2	7	from	changes	380:386	arg1	composition					412:422	the fecal microbiome composition	391:422	the fecal microbiome composition	391:422	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	6	8	theme	genus	1062:1066	arg1	level					1068:1072	the genus level	1058:1072	the genus level	1058:1072	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	10	9	theme	study	1995:1999	arg1	groups					2001:2006	all study groups	1991:2006	all study groups	1991:2006	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	3	10	theme	enrofloxacin	715:726	arg1	control					669:675	control	669:675	control (no treatment)	669:690	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	10	theme	enrofloxacin	715:726	arg1	treatment					681:689	no treatment	678:689	no treatment	678:689	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	10	theme	enrofloxacin	715:726	arg1	groups					661:666	one of three study groups	642:666	one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin	642:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	10	theme	enrofloxacin	715:726	arg1	treatment					702:710	a single treatment	693:710	a single treatment of enrofloxacin	693:726	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	10	theme	enrofloxacin	715:726	arg1	treatment					741:749	a single treatment	732:749	a single treatment of tulathromycin	732:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	2	11	theme	shotgun	340:346	arg1	sequencing					348:357	shotgun sequencing	340:357	shotgun sequencing	340:357	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	4	12	theme	DNA	851:853	arg1	extraction					855:864	DNA extraction	851:864	DNA extraction	851:864	Fecal samples were collected at days 4, 14, 56 and 112 days after enrollment, and DNA extraction and sequencing was conducted.					
31825967	0	13	theme	calves	118:123	arg1	composition					76:86	fecal microbiota composition	59:86	fecal microbiota composition	59:86	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	0	13	theme	calves	118:123	arg1	function					100:107	genetic function	92:107	genetic function	92:107	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	8	14	theme	relevance	1630:1638	arg1	"					1624:1624	"Erythromycin resistance"	1600:1624	"Erythromycin resistance"	1600:1624	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	8	14	theme	relevance	1630:1638	arg1	"					1594:1594	"Resistance to Fluoroquinolones"	1563:1594	"Resistance to Fluoroquinolones"	1563:1594	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	6	15	theme	Coprococcus	1136:1146	arg1	abundance					1114:1122	higher relative abundance	1098:1122	higher relative abundance of Blautia, Coprococcus and Desulfovibrio	1098:1164	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	6	15	theme	Coprococcus	1136:1146	arg1	abundance					1176:1184	lower abundance	1170:1184	lower abundance of Bacteroides	1170:1199	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	3	16	theme	single	734:739	arg1	groups					661:666	one of three study groups	642:666	one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin	642:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	16	theme	single	734:739	arg1	treatment					741:749	a single treatment	732:749	a single treatment of tulathromycin	732:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	6	17	theme	Blautia	1127:1133	arg1	abundance					1114:1122	higher relative abundance	1098:1122	higher relative abundance of Blautia, Coprococcus and Desulfovibrio	1098:1164	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	6	17	theme	Blautia	1127:1133	arg1	abundance					1176:1184	lower abundance	1170:1184	lower abundance of Bacteroides	1170:1199	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	8	18	from	abundance	1709:1717	arg1	different					1687:1695	different	1687:1695	different	1687:1695	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	6	19	theme	other	1218:1222	arg1	groups					1230:1235	other study groups	1218:1235	other study groups	1218:1235	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	7	20	theme	resistance	1494:1503	arg1	genes					1505:1509	antibiotic resistance genes	1483:1509	antibiotic resistance genes	1483:1509	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	11	21	theme	genetic	2106:2112	arg1	microbiota					2125:2134	genetic functional microbiota	2106:2134	genetic functional microbiota	2106:2134	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	6	22	theme	higher	1098:1103	arg1	abundance					1114:1122	higher relative abundance	1098:1122	higher relative abundance of Blautia, Coprococcus and Desulfovibrio	1098:1164	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	1	23	theme	antimicrobial	272:284	arg1	resistance					286:295	antimicrobial resistance	272:295	antimicrobial resistance using new sequencing technologies	272:329	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	2	24	theme	calves	510:515	arg1	functions					454:462	functions	454:462	functions	454:462	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	11	25	theme	study	2155:2159	arg1	period					2161:2166	the study period	2151:2166	the study period	2151:2166	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	11	26	theme	calves	2139:2144	arg1	composition					2090:2100	microbial composition	2080:2100	microbial composition	2080:2100	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	11	26	theme	calves	2139:2144	arg1	microbiota					2125:2134	genetic functional microbiota	2106:2134	genetic functional microbiota	2106:2134	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	0	27	from	Effects	0:6	arg1	composition					76:86	fecal microbiota composition	59:86	fecal microbiota composition	59:86	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	0	27	from	Effects	0:6	arg1	function					100:107	genetic function	92:107	genetic function	92:107	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	1	28	dep	The	126:128	arg1	concerns					141:148	concerns	141:148	concerns	141:148	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	0	29	theme	genetic	92:98	arg1	function					100:107	genetic function	92:107	genetic function	92:107	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	7	30	with	treatment	1517:1525	arg1	enrofloxacin					1532:1543	enrofloxacin	1532:1543	enrofloxacin	1532:1543	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	5	31	theme	antibiotic	910:919	arg1	treatment					921:929	antibiotic treatment	910:929	antibiotic treatment	910:929	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	7	32	theme	SEED	1242:1245	arg1	database					1247:1254	The SEED database	1238:1254	The SEED database	1238:1254	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	11	33	dep	composition	2090:2100	arg1	the					2076:2078	the	2076:2078	the	2076:2078	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	0	34	theme	treatment	11:19	arg1	Effects					0:6	Effects	0:6	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves	0:123	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	1	35	theme	sequencing	307:316	arg1	technologies					318:329	new sequencing technologies	303:329	new sequencing technologies	303:329	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	7	36	theme	toxic	1424:1428	arg1	compounds					1430:1438	toxic compounds	1424:1438	toxic compounds	1424:1438	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	5	37	theme	genetic	949:955	arg1	level					968:972	genetic functional level	949:972	genetic functional level	949:972	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	0	38	theme	fecal	59:63	arg1	composition					76:86	fecal microbiota composition	59:86	fecal microbiota composition	59:86	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	0	39	with	treatment	11:19	arg1	tulathromycin					42:54	tulathromycin	42:54	tulathromycin	42:54	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	0	39	with	treatment	11:19	arg1	enrofloxacin					26:37	enrofloxacin	26:37	enrofloxacin	26:37	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	7	40	theme	Resistance	1394:1403	arg1	abundance					1380:1388	a higher relative abundance	1362:1388	a higher relative abundance of "Resistance to antibiotics and toxic compounds	1362:1438	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	10	41	theme	genetic	1946:1952	arg1	profile					1965:1971	genetic functional profile	1946:1971	genetic functional profile	1946:1971	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	3	42	theme	study	655:659	arg1	treatment					702:710	a single treatment	693:710	a single treatment of enrofloxacin	693:726	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	42	theme	study	655:659	arg1	groups					661:666	one of three study groups	642:666	one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin	642:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	42	theme	study	655:659	arg1	control					669:675	control	669:675	control (no treatment)	669:690	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	42	theme	study	655:659	arg1	treatment					741:749	a single treatment	732:749	a single treatment of tulathromycin	732:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	4	43	theme	Fecal	769:773	arg1	samples					775:781	Fecal samples	769:781	Fecal samples	769:781	Fecal samples were collected at days 4, 14, 56 and 112 days after enrollment, and DNA extraction and sequencing was conducted.					
31825967	2	44	theme	fecal	395:399	arg1	composition					412:422	the fecal microbiome composition	391:422	the fecal microbiome composition	391:422	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	1	45	theme	use	252:254	arg1	impacts					227:233	the impacts	223:233	the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies	223:329	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	2	46	from	focus	445:449	arg1	functions					454:462	functions	454:462	functions	454:462	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	8	47	theme	Resistance	1564:1573	arg1	"					1594:1594	"Resistance to Fluoroquinolones"	1563:1594	"Resistance to Fluoroquinolones"	1563:1594	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	7	48	theme	relative	1371:1378	arg1	abundance					1380:1388	a higher relative abundance	1362:1388	a higher relative abundance of "Resistance to antibiotics and toxic compounds	1362:1438	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	5	49	from	effect	900:905	arg1	taxon					939:943	taxon	939:943	taxon	939:943	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	5	49	from	effect	900:905	arg1	level					968:972	genetic functional level	949:972	genetic functional level	949:972	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	3	50	dep	groups	661:666	arg1	treatment					702:710	a single treatment	693:710	a single treatment of enrofloxacin	693:726	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	50	dep	groups	661:666	arg1	treatment					681:689	no treatment	678:689	no treatment	678:689	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	50	dep	groups	661:666	arg1	groups					661:666	one of three study groups	642:666	one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin	642:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	50	dep	groups	661:666	arg1	control					669:675	control	669:675	control (no treatment)	669:690	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	50	dep	groups	661:666	arg1	treatment					741:749	a single treatment	732:749	a single treatment of tulathromycin	732:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	6	51	theme	enrofloxacin	1075:1086	arg1	group					1088:1092	enrofloxacin group	1075:1092	enrofloxacin group	1075:1092	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	9	52	theme	study	1795:1799	arg1	period					1801:1806	the study period	1791:1806	the study period regardless of study group	1791:1832	"Resistance to fluoroquinolones" increased during the study period regardless of study group.					
31825967	5	53	dep	time	977:980	arg1	including					983:991	including	983:991	including Day 0 as a covariate	983:1012	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	3	54	theme	single	695:700	arg1	treatment					702:710	a single treatment	693:710	a single treatment of enrofloxacin	693:726	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	54	theme	single	695:700	arg1	groups					661:666	one of three study groups	642:666	one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin	642:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	6	55	theme	lower	1170:1174	arg1	abundance					1176:1184	lower abundance	1170:1184	lower abundance of Bacteroides	1170:1199	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	3	56	theme	old	539:541	arg1	Heifers					518:524	Heifers	518:524	Heifers	518:524	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	6	57	theme	Desulfovibrio	1152:1164	arg1	abundance					1114:1122	higher relative abundance	1098:1122	higher relative abundance of Blautia, Coprococcus and Desulfovibrio	1098:1164	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	6	57	theme	Desulfovibrio	1152:1164	arg1	abundance					1176:1184	lower abundance	1170:1184	lower abundance of Bacteroides	1170:1199	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	8	58	theme	Erythromycin	1601:1612	arg1	"					1624:1624	"Erythromycin resistance"	1600:1624	"Erythromycin resistance"	1600:1624	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	9	59	theme	study	1822:1826	arg1	group					1828:1832	study group	1822:1832	study group	1822:1832	"Resistance to fluoroquinolones" increased during the study period regardless of study group.					
31825967	5	60	dep	taxon	939:943	arg1	each					934:937	each	934:937	each	934:937	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	10	61	theme	small	1843:1847	arg1	differences					1849:1859	small differences	1843:1859	small differences over the first weeks between study groups	1843:1901	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	1	62	from	livestock	259:267	arg1	impacts					227:233	the impacts	223:233	the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies	223:329	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	6	63	theme	study	1224:1228	arg1	groups					1230:1235	other study groups	1218:1235	other study groups	1218:1235	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	1	64	from	impacts	227:233	arg1	livestock					259:267	livestock	259:267	livestock	259:267	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	1	64	from	impacts	227:233	arg1	resistance					286:295	antimicrobial resistance	272:295	antimicrobial resistance using new sequencing technologies	272:329	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	7	65	theme	antibiotic	1483:1492	arg1	genes					1505:1509	antibiotic resistance genes	1483:1509	antibiotic resistance genes	1483:1509	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	7	66	theme	functional	1277:1286	arg1	analyses					1288:1295	genetic functional analyses	1269:1295	genetic functional analyses	1269:1295	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	10	67	theme	first	1870:1874	arg1	weeks					1876:1880	the first weeks	1866:1880	the first weeks between study groups	1866:1901	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	8	68	theme	study	1650:1654	arg1	groups					1656:1661	the study groups	1646:1661	the study groups	1646:1661	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	6	69	theme	relative	1105:1112	arg1	abundance					1114:1122	higher relative abundance	1098:1122	higher relative abundance of Blautia, Coprococcus and Desulfovibrio	1098:1164	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	8	70	from	different	1687:1695	arg1	abundance					1709:1717	relative abundance	1700:1717	relative abundance	1700:1717	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	0	71	theme	microbiota	65:74	arg1	composition					76:86	fecal microbiota composition	59:86	fecal microbiota composition	59:86	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	7	72	from	calves	1316:1321	arg1	group					1343:1347	the enrofloxacin group	1326:1347	the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds	1326:1438	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	11	73	theme	functional	2114:2123	arg1	microbiota					2125:2134	genetic functional microbiota	2106:2134	genetic functional microbiota	2106:2134	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	3	74	theme	tulathromycin	754:766	arg1	control					669:675	control	669:675	control (no treatment)	669:690	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	74	theme	tulathromycin	754:766	arg1	treatment					681:689	no treatment	678:689	no treatment	678:689	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	74	theme	tulathromycin	754:766	arg1	groups					661:666	one of three study groups	642:666	one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin	642:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	74	theme	tulathromycin	754:766	arg1	treatment					702:710	a single treatment	693:710	a single treatment of enrofloxacin	693:726	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	74	theme	tulathromycin	754:766	arg1	treatment					741:749	a single treatment	732:749	a single treatment of tulathromycin	732:766	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	1	75	theme	antimicrobial	155:167	arg1	resistance					169:178	antimicrobial resistance	155:178	antimicrobial resistance	155:178	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	2	76	theme	dairy	504:508	arg1	calves					510:515	dairy calves	504:515	dairy calves	504:515	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	7	77	used	used	1260:1263	arg2	database					1247:1254	The SEED database	1238:1254	The SEED database	1238:1254	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	10	78	theme	microbiota	1919:1928	arg1	similar					1977:1983	similar	1977:1983	similar	1977:1983	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	10	78	theme	microbiota	1919:1928	arg1	composition					1930:1940	the microbiota composition	1915:1940	the microbiota composition	1915:1940	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	0	79	theme	dairy	112:116	arg1	calves					118:123	dairy calves	112:123	dairy calves	112:123	Effects of treatment with enrofloxacin or tulathromycin on fecal microbiota composition and genetic function of dairy calves.					
31825967	8	80	theme	relative	1700:1707	arg1	abundance					1709:1717	relative abundance	1700:1717	relative abundance	1700:1717	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	5	81	theme	few	1024:1026	arg1	changes					1028:1034	few changes	1024:1034	few changes in the microbiota	1024:1052	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	2	82	theme	related	464:470	arg1	functions					454:462	functions	454:462	functions	454:462	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	2	83	theme	microbiome	401:410	arg1	composition					412:422	the fecal microbiome composition	391:422	the fecal microbiome composition	391:422	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	1	84	theme	new	303:305	arg1	technologies					318:329	new sequencing technologies	303:329	new sequencing technologies	303:329	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	3	85	dep	3	531:531	arg1	to					528:529	to	528:529	to	528:529	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	10	86	from	Day	1907:1909	arg1	similar					1977:1983	similar	1977:1983	similar	1977:1983	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	10	86	from	Day	1907:1909	arg1	composition					1930:1940	the microbiota composition	1915:1940	the microbiota composition	1915:1940	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	11	87	theme	microbial	2080:2088	arg1	composition					2090:2100	microbial composition	2080:2100	microbial composition	2080:2100	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	6	88	contain	had	1094:1096	arg2	abundance					1114:1122	higher relative abundance	1098:1122	higher relative abundance of Blautia, Coprococcus and Desulfovibrio	1098:1164	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	6	88	contain	had	1094:1096	arg2	abundance					1176:1184	lower abundance	1170:1184	lower abundance of Bacteroides	1170:1199	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	6	88	contain	had	1094:1096	arg1	group					1088:1092	enrofloxacin group	1075:1092	enrofloxacin group	1075:1092	At the genus level, enrofloxacin group had higher relative abundance of Blautia, Coprococcus and Desulfovibrio and lower abundance of Bacteroides when compared to other study groups.					
31825967	5	89	from	changes	1028:1034	arg1	microbiota					1043:1052	the microbiota	1039:1052	the microbiota	1039:1052	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	10	90	theme	study	1890:1894	arg1	groups					1896:1901	study groups	1890:1901	study groups	1890:1901	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	5	91	theme	functional	957:966	arg1	level					968:972	genetic functional level	949:972	genetic functional level	949:972	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	2	92	theme	antimicrobial	475:487	arg1	resistance					489:498	antimicrobial resistance	475:498	antimicrobial resistance	475:498	Through shotgun sequencing, we investigated the changes in the fecal microbiome composition and function, with a focus on functions related to antimicrobial resistance, of dairy calves.					
31825967	11	93	from	impacts	2065:2071	arg1	composition					2090:2100	microbial composition	2080:2100	microbial composition	2080:2100	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	11	93	from	impacts	2065:2071	arg1	microbiota					2125:2134	genetic functional microbiota	2106:2134	genetic functional microbiota	2106:2134	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	7	94	from	increase	1471:1478	arg1	genes					1505:1509	antibiotic resistance genes	1483:1509	antibiotic resistance genes	1483:1509	The SEED database was used for genetic functional analyses, which showed that calves in the enrofloxacin group started with a higher relative abundance of "Resistance to antibiotics and toxic compounds" function on Day 0, however an increase in antibiotic resistance genes after treatment with enrofloxacin was not observed.					
31825967	8	95	theme	study	1727:1731	arg1	groups					1733:1738	study groups	1727:1738	study groups	1727:1738	"Resistance to Fluoroquinolones" and "Erythromycin resistance", of relevance given the study groups, were not statistically different in relative abundance between study groups.					
31825967	5	96	theme	treatment	921:929	arg1	effect					900:905	The effect	896:905	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate)	896:1013	The effect of antibiotic treatment on each taxon and genetic functional level by time (including Day 0 as a covariate) revealed few changes in the microbiota.					
31825967	10	97	theme	functional	1954:1963	arg1	profile					1965:1971	genetic functional profile	1946:1971	genetic functional profile	1946:1971	Despite small differences over the first weeks between study groups, at Day 112 the microbiota composition and genetic functional profile was similar among all study groups.					
31825967	1	98	theme	antimicrobial	238:250	arg1	use					252:254	antimicrobial use	238:254	antimicrobial use in livestock	238:267	The increasing concerns with antimicrobial resistance highlights the need for studies evaluating the impacts of antimicrobial use in livestock on antimicrobial resistance using new sequencing technologies.					
31825967	3	99	theme	no	678:679	arg1	control					669:675	control	669:675	control (no treatment)	669:690	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	3	99	theme	no	678:679	arg1	treatment					681:689	no treatment	678:689	no treatment	678:689	Heifers 2 to 3 weeks old, which were not treated with antibiotics by the farm before enrollment, were randomly allocated to one of three study groups: control (no treatment), a single treatment of enrofloxacin, or a single treatment of tulathromycin.					
31825967	11	100	contain	had	2053:2055	arg1	tulathromycin					2039:2051	tulathromycin	2039:2051	tulathromycin	2039:2051	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	11	100	contain	had	2053:2055	arg2	impacts					2065:2071	minimal impacts	2057:2071	minimal impacts on the microbial composition and genetic functional microbiota of calves	2057:2144	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31825967	11	100	contain	had	2053:2055	arg1	enrofloxacin					2023:2034	enrofloxacin	2023:2034	enrofloxacin	2023:2034	In our study, enrofloxacin or tulathromycin had minimal impacts on the microbial composition and genetic functional microbiota of calves over the study period.					
31575491	2	0	theme	vaccine	496:502	arg1	candidates					504:513	vaccine candidates	496:513	vaccine candidates	496:513	Adjuvants have these properties helping vaccine candidates to induce a strong, durable, and fast immune response.					
31575491	8	1	theme	LBIFA	1394:1398	arg1	groups					1415:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups.					
31575491	0	2	theme	braziliensis	166:177	arg1	antigens					179:186	Leishmania (Viannia) braziliensis antigens	145:186	Leishmania (Viannia) braziliensis antigens	145:186	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	5	3	theme	higher	1044:1049	arg1	ability					1051:1057	higher ability	1044:1057	higher ability to increase the capacity of APCs to present antigens	1044:1110	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	5	4	theme	increased	1118:1126	arg1	frequency					1128:1136	increased frequency	1118:1136	increased frequency of CD11c+CD86+ cells	1118:1157	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	4	5	theme	specific	828:835	arg1	response					844:851	The specific immune response	824:851	The specific immune response induced by these compositions	824:881	The specific immune response induced by these compositions demonstrated that they were powerfully immunogenic, increasing cellular infiltration in the skin.					
31575491	0	6	with	saponin	75:81	arg1	antigens					179:186	Leishmania (Viannia) braziliensis antigens	145:186	Leishmania (Viannia) braziliensis antigens	145:186	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	0	7	from	response	53:60	arg1	recruitment					19:29	cellular recruitment	10:29	cellular recruitment	10:29	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	8	8	theme	MPL	1401:1403	arg1	groups					1415:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups.					
31575491	8	9	theme	cytokines	1352:1360	arg1	production					1362:1371	higher cytokines production	1345:1371	higher cytokines production	1345:1371	At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups.					
31575491	0	10	theme	monophosphoryl	84:97	arg1	lipid-A					99:105	monophosphoryl lipid-A	84:105	monophosphoryl lipid-A	84:105	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	0	11	from	Effect	0:5	arg1	recruitment					19:29	cellular recruitment	10:29	cellular recruitment	10:29	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	1	12	theme	vaccine	438:444	arg1	response					446:453	vaccine response	438:453	vaccine response	438:453	The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response.					
31575491	9	13	theme	potential	1469:1477	arg1	LBMPL					1459:1463	LBMPL	1459:1463	LBMPL	1459:1463	Our data demonstrate that LBSAP and LBMPL are potential formulations to be tested in other experimental models.					
31575491	9	13	theme	potential	1469:1477	arg1	formulations					1479:1490	potential formulations	1469:1490	potential formulations	1469:1490	Our data demonstrate that LBSAP and LBMPL are potential formulations to be tested in other experimental models.					
31575491	9	13	theme	potential	1469:1477	arg1	LBSAP					1449:1453	LBSAP	1449:1453	LBSAP	1449:1453	Our data demonstrate that LBSAP and LBMPL are potential formulations to be tested in other experimental models.					
31575491	3	14	theme	total	761:765	arg1	antigen					767:773	total antigen	761:773	total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL	761:821	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	2	15	theme	strong	527:532	arg1	response					560:567	a strong, durable, and fast immune response	525:567	a strong, durable, and fast immune response	525:567	Adjuvants have these properties helping vaccine candidates to induce a strong, durable, and fast immune response.					
31575491	5	16	theme	CD11c+CD86+	1141:1151	arg1	cells					1153:1157	CD11c+CD86+ cells	1141:1157	CD11c+CD86+ cells	1141:1157	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	1	17	theme	vaccine	356:362	arg1	candidates					364:373	vaccine candidates	356:373	vaccine candidates	356:373	The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response.					
31575491	0	18	theme	vaccine	194:200	arg1	formulation					202:212	a vaccine formulation	192:212	a vaccine formulation	192:212	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	3	19	theme	braziliensis	781:792	arg1	antigen					767:773	total antigen	761:773	total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL	761:821	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	8	20	theme	IFA	1389:1391	arg1	groups					1415:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups.					
31575491	1	21	theme	candidates	364:373	arg1	safety					346:351	safety	346:351	safety of vaccine candidates	346:373	The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response.					
31575491	1	21	theme	candidates	364:373	arg1	response					333:340	the immune response	322:340	the immune response	322:340	The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response.					
31575491	9	22	theme	other	1508:1512	arg1	models					1527:1532	other experimental models	1508:1532	other experimental models	1508:1532	Our data demonstrate that LBSAP and LBMPL are potential formulations to be tested in other experimental models.					
31575491	6	23	theme	CD25	1254:1257	arg1	expression					1231:1240	lymphocytes increasing expression	1208:1240	lymphocytes increasing expression of CD69 and CD25	1208:1257	SAP, MPL, LBSAP, LBIFA and LBMPL could activate lymphocytes increasing expression of CD69 and CD25.					
31575491	3	24	theme	Adjuvant	682:689	arg1	Freund					673:678	Incomplete Freund's Adjuvant (IFA)	662:695	Incomplete Freund's Adjuvant (IFA)	662:695	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	3	24	theme	Adjuvant	682:689	arg1	IFA					692:694	IFA	692:694	IFA	692:694	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	0	25	with	lipid-A	99:105	arg1	antigens					179:186	Leishmania (Viannia) braziliensis antigens	145:186	Leishmania (Viannia) braziliensis antigens	145:186	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	6	26	theme	increasing	1220:1229	arg1	expression					1231:1240	lymphocytes increasing expression	1208:1240	lymphocytes increasing expression of CD69 and CD25	1208:1257	SAP, MPL, LBSAP, LBIFA and LBMPL could activate lymphocytes increasing expression of CD69 and CD25.					
31575491	6	27	theme	CD69	1245:1248	arg1	expression					1231:1240	lymphocytes increasing expression	1208:1240	lymphocytes increasing expression of CD69 and CD25	1208:1257	SAP, MPL, LBSAP, LBIFA and LBMPL could activate lymphocytes increasing expression of CD69 and CD25.					
31575491	3	28	theme	Monophosphoryl	701:714	arg1	MPL-SE®					728:734	MPL-SE®	728:734	MPL-SE®	728:734	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	3	28	theme	Monophosphoryl	701:714	arg1	SE					724:725	Monophosphoryl lipid-A SE	701:725	Monophosphoryl lipid-A SE (MPL-SE®)	701:735	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	5	29	theme	nodes	996:1000	arg1	cultures					1002:1009	Draining lymph nodes cultures	981:1009	Draining lymph nodes cultures	981:1009	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	10	30	theme	safe	1670:1673	arg1	vaccines					1702:1709	safe, immunogenic and effective vaccines	1670:1709	safe, immunogenic and effective vaccines	1670:1709	Also, the data obtained could expand the knowledge about immune response after sensitization and also contribute to the development of safe, immunogenic and effective vaccines.					
31575491	3	31	theme	lipid-A	716:722	arg1	MPL-SE®					728:734	MPL-SE®	728:734	MPL-SE®	728:734	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	3	31	theme	lipid-A	716:722	arg1	SE					724:725	Monophosphoryl lipid-A SE	701:725	Monophosphoryl lipid-A SE (MPL-SE®)	701:735	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	2	32	theme	fast	548:551	arg1	response					560:567	a strong, durable, and fast immune response	525:567	a strong, durable, and fast immune response	525:567	Adjuvants have these properties helping vaccine candidates to induce a strong, durable, and fast immune response.					
31575491	7	33	theme	excellent	1279:1287	arg1	group					1266:1270	LBSAP group	1260:1270	LBSAP group	1260:1270	LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h.					
31575491	7	33	theme	excellent	1279:1287	arg1	inducer					1289:1295	an excellent inducer	1276:1295	an excellent inducer of pro-inflammatory cytokines	1276:1325	LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h.					
31575491	7	34	theme	pro-inflammatory	1300:1315	arg1	cytokines					1317:1325	pro-inflammatory cytokines	1300:1325	pro-inflammatory cytokines	1300:1325	LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h.					
31575491	3	35	dep	braziliensis	781:792	arg1	LBSAP					800:804	LBSAP	800:804	LBSAP	800:804	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	3	35	dep	braziliensis	781:792	arg1	LBMPL					817:821	LBMPL	817:821	LBMPL	817:821	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	3	35	dep	braziliensis	781:792	arg1	LBIFA					807:811	LBIFA	807:811	LBIFA	807:811	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	0	36	theme	cellular	10:17	arg1	recruitment					19:29	cellular recruitment	10:29	cellular recruitment	10:29	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	8	37	theme	LBMPL	1409:1413	arg1	groups					1415:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups.					
31575491	0	38	theme	Incomplete	111:120	arg1	Freund					122:127	Incomplete Freund's Adjuvant	111:138	Incomplete Freund's Adjuvant	111:138	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	3	39	theme	adjuvants	630:638	arg1	response					618:625	the specific immune response	598:625	the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL	598:821	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	7	40	theme	cytokines	1317:1325	arg1	group					1266:1270	LBSAP group	1260:1270	LBSAP group	1260:1270	LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h.					
31575491	7	40	theme	cytokines	1317:1325	arg1	inducer					1289:1295	an excellent inducer	1276:1295	an excellent inducer of pro-inflammatory cytokines	1276:1325	LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h.					
31575491	0	41	theme	Adjuvant	131:138	arg1	Freund					122:127	Incomplete Freund's Adjuvant	111:138	Incomplete Freund's Adjuvant	111:138	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	8	42	theme	higher	1345:1350	arg1	production					1362:1371	higher cytokines production	1345:1371	higher cytokines production	1345:1371	At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups.					
31575491	10	43	theme	vaccines	1702:1709	arg1	development					1655:1665	the development	1651:1665	the development of safe, immunogenic and effective vaccines	1651:1709	Also, the data obtained could expand the knowledge about immune response after sensitization and also contribute to the development of safe, immunogenic and effective vaccines.					
31575491	7	44	from	24 h	1330:1333	arg1	group					1266:1270	LBSAP group	1260:1270	LBSAP group	1260:1270	LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h.					
31575491	7	44	from	24 h	1330:1333	arg1	inducer					1289:1295	an excellent inducer	1276:1295	an excellent inducer of pro-inflammatory cytokines	1276:1325	LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h.					
31575491	1	45	theme	strategies	300:309	arg1	development					285:295	the development	281:295	the development of strategies	281:309	The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response.					
31575491	2	46	theme	durable	535:541	arg1	response					560:567	a strong, durable, and fast immune response	525:567	a strong, durable, and fast immune response	525:567	Adjuvants have these properties helping vaccine candidates to induce a strong, durable, and fast immune response.					
31575491	10	47	theme	effective	1692:1700	arg1	vaccines					1702:1709	safe, immunogenic and effective vaccines	1670:1709	safe, immunogenic and effective vaccines	1670:1709	Also, the data obtained could expand the knowledge about immune response after sensitization and also contribute to the development of safe, immunogenic and effective vaccines.					
31575491	0	48	theme	immune	46:51	arg1	response					53:60	the innate immune response	35:60	the innate immune response	35:60	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	3	49	from	response	618:625	arg1	combination					744:754	combination	744:754	combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL	744:821	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	0	50	theme	Leishmania	145:154	arg1	antigens					179:186	Leishmania (Viannia) braziliensis antigens	145:186	Leishmania (Viannia) braziliensis antigens	145:186	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	1	51	theme	intense	392:398	arg1	search					400:405	an intense search	389:405	an intense search for substances that potentiate vaccine response	389:453	The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response.					
31575491	6	52	theme	lymphocytes	1208:1218	arg1	expression					1231:1240	lymphocytes increasing expression	1208:1240	lymphocytes increasing expression of CD69 and CD25	1208:1257	SAP, MPL, LBSAP, LBIFA and LBMPL could activate lymphocytes increasing expression of CD69 and CD25.					
31575491	10	53	theme	immune	1592:1597	arg1	response					1599:1606	immune response	1592:1606	immune response after sensitization	1592:1626	Also, the data obtained could expand the knowledge about immune response after sensitization and also contribute to the development of safe, immunogenic and effective vaccines.					
31575491	0	54	theme	innate	39:44	arg1	response					53:60	the innate immune response	35:60	the innate immune response	35:60	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	5	55	theme	APCs	1087:1090	arg1	capacity					1075:1082	the capacity	1071:1082	the capacity of APCs to present antigens	1071:1110	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	3	56	theme	Incomplete	662:671	arg1	Freund					673:678	Incomplete Freund's Adjuvant (IFA)	662:695	Incomplete Freund's Adjuvant (IFA)	662:695	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	3	56	theme	Incomplete	662:671	arg1	IFA					692:694	IFA	692:694	IFA	692:694	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	5	57	theme	Draining	981:988	arg1	nodes					996:1000	Draining lymph nodes	981:1000	Draining lymph nodes cultures	981:1009	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	10	58	theme	immunogenic	1676:1686	arg1	vaccines					1702:1709	safe, immunogenic and effective vaccines	1670:1709	safe, immunogenic and effective vaccines	1670:1709	Also, the data obtained could expand the knowledge about immune response after sensitization and also contribute to the development of safe, immunogenic and effective vaccines.					
31575491	0	59	theme	Viannia	157:163	arg1	antigens					179:186	Leishmania (Viannia) braziliensis antigens	145:186	Leishmania (Viannia) braziliensis antigens	145:186	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	2	60	contain	have	466:469	arg2	properties					477:486	these properties	471:486	these properties	471:486	Adjuvants have these properties helping vaccine candidates to induce a strong, durable, and fast immune response.					
31575491	2	60	contain	have	466:469	arg1	Adjuvants					456:464	Adjuvants	456:464	Adjuvants	456:464	Adjuvants have these properties helping vaccine candidates to induce a strong, durable, and fast immune response.					
31575491	3	61	theme	specific	602:609	arg1	response					618:625	the specific immune response	598:625	the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL	598:821	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	5	62	theme	cells	1153:1157	arg1	frequency					1128:1136	increased frequency	1118:1136	increased frequency of CD11c+CD86+ cells	1118:1157	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	2	63	theme	immune	553:558	arg1	response					560:567	a strong, durable, and fast immune response	525:567	a strong, durable, and fast immune response	525:567	Adjuvants have these properties helping vaccine candidates to induce a strong, durable, and fast immune response.					
31575491	5	64	theme	lymph	990:994	arg1	nodes					996:1000	Draining lymph nodes	981:1000	Draining lymph nodes cultures	981:1009	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	3	65	theme	immune	611:616	arg1	response					618:625	the specific immune response	598:625	the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL	598:821	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	0	66	with	Freund	122:127	arg1	antigens					179:186	Leishmania (Viannia) braziliensis antigens	145:186	Leishmania (Viannia) braziliensis antigens	145:186	Effect on cellular recruitment and the innate immune response by combining saponin, monophosphoryl lipid-A and Incomplete Freund's Adjuvant with Leishmania (Viannia) braziliensis antigens for a vaccine formulation.					
31575491	1	67	theme	poor	219:222	arg1	immunogenicity					224:237	The poor immunogenicity	215:237	The poor immunogenicity displayed by some antigens	215:264	The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response.					
31575491	4	68	theme	immune	837:842	arg1	response					844:851	The specific immune response	824:851	The specific immune response induced by these compositions	824:881	The specific immune response induced by these compositions demonstrated that they were powerfully immunogenic, increasing cellular infiltration in the skin.					
31575491	9	69	theme	experimental	1514:1525	arg1	models					1527:1532	other experimental models	1508:1532	other experimental models	1508:1532	Our data demonstrate that LBSAP and LBMPL are potential formulations to be tested in other experimental models.					
31575491	5	70	theme	present	1095:1101	arg1	antigens					1103:1110	present antigens	1095:1110	present antigens	1095:1110	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	4	71	theme	cellular	946:953	arg1	infiltration					955:966	cellular infiltration	946:966	cellular infiltration	946:966	The specific immune response induced by these compositions demonstrated that they were powerfully immunogenic, increasing cellular infiltration in the skin.					
31575491	1	72	theme	immune	326:331	arg1	response					333:340	the immune response	322:340	the immune response	322:340	The poor immunogenicity displayed by some antigens has encouraged the development of strategies to improve the immune response and safety of vaccine candidates, resulting in an intense search for substances that potentiate vaccine response.					
31575491	7	73	theme	LBSAP	1260:1264	arg1	group					1266:1270	LBSAP group	1260:1270	LBSAP group	1260:1270	LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h.					
31575491	7	73	theme	LBSAP	1260:1264	arg1	inducer					1289:1295	an excellent inducer	1276:1295	an excellent inducer of pro-inflammatory cytokines	1276:1325	LBSAP group was an excellent inducer of pro-inflammatory cytokines at 24 h.					
31575491	3	74	with	combination	744:754	arg1	antigen					767:773	total antigen	761:773	total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL	761:821	In this study, we evaluated the specific immune response of adjuvants alone, Saponin (SAP), Incomplete Freund's Adjuvant (IFA) and Monophosphoryl lipid-A SE (MPL-SE®) and in combination with total antigen of L. braziliensis (LB): LBSAP, LBIFA and LBMPL.					
31575491	8	75	located	observed	1377:1384	arg1	groups					1415:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	IFA, LBIFA, MPL and LBMPL groups	1389:1420	At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups.					
31575491	8	75	located	observed	1377:1384	arg1	48 h					1339:1342	48 h	1339:1342	48 h	1339:1342	At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups.					
31575491	8	75	located	observed	1377:1384	arg2	production					1362:1371	higher cytokines production	1345:1371	higher cytokines production	1345:1371	At 48 h, higher cytokines production was observed in IFA, LBIFA, MPL and LBMPL groups.					
31575491	5	76	contain	have	1039:1042	arg1	LBMPL					1033:1037	LBMPL	1033:1037	LBMPL	1033:1037	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	5	76	contain	have	1039:1042	arg2	ability					1051:1057	higher ability	1044:1057	higher ability to increase the capacity of APCs to present antigens	1044:1110	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
31575491	5	76	contain	have	1039:1042	arg1	LBIFA					1023:1027	LBIFA	1023:1027	LBIFA	1023:1027	Draining lymph nodes cultures showed that LBIFA and LBMPL have higher ability to increase the capacity of APCs to present antigens, with increased frequency of CD11c+CD86+ cells.					
30472620	8	0	theme	study	1596:1600	arg1	period					1586:1591	the period	1582:1591	the period of study	1582:1600	Analysis of pH during heating and holding at 60 °C indicated that SMUF acidified continuously under the period of study, while acidification in BCC/SMUF mixtures terminated after a short period, indicating that the type of CaP formed on heating is altered in the presence of BCC.					
30472620	4	1	theme	various	669:675	arg1	media					684:688	various liquid media	669:688	various liquid media at pH 6.8	669:698	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	1	2	theme	serum	269:273	arg1	composition					275:285	serum composition	269:285	serum composition	269:285	Monomeric bovine β-casein self-associates into micelles under appropriate conditions of protein concentration, serum composition and temperature.					
30472620	8	3	theme	BCC	1757:1759	arg1	presence					1745:1752	the presence	1741:1752	the presence of BCC	1741:1759	Analysis of pH during heating and holding at 60 °C indicated that SMUF acidified continuously under the period of study, while acidification in BCC/SMUF mixtures terminated after a short period, indicating that the type of CaP formed on heating is altered in the presence of BCC.					
30472620	1	4	theme	Monomeric	158:166	arg1	β-casein					175:182	Monomeric bovine β-casein	158:182	Monomeric bovine β-casein	158:182	Monomeric bovine β-casein self-associates into micelles under appropriate conditions of protein concentration, serum composition and temperature.					
30472620	6	5	theme	turbidity	1304:1312	arg1	development					1314:1324	turbidity development	1304:1324	turbidity development in BCC solutions	1304:1341	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	1	6	theme	composition	275:285	arg1	conditions					232:241	appropriate conditions	220:241	appropriate conditions of protein concentration, serum composition and temperature	220:301	Monomeric bovine β-casein self-associates into micelles under appropriate conditions of protein concentration, serum composition and temperature.					
30472620	8	7	from	acidification	1609:1621	arg1	mixtures					1635:1642	BCC/SMUF mixtures	1626:1642	BCC/SMUF mixtures	1626:1642	Analysis of pH during heating and holding at 60 °C indicated that SMUF acidified continuously under the period of study, while acidification in BCC/SMUF mixtures terminated after a short period, indicating that the type of CaP formed on heating is altered in the presence of BCC.					
30472620	5	8	from	calcium	874:880	arg1	thermo-reversible					812:828	thermo-reversible	812:828	thermo-reversible	812:828	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	6	9	theme	most	903:906	arg1	phases					914:919	most serum phases	903:919	most serum phases	903:919	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	8	10	theme	pH	1494:1495	arg1	Analysis					1482:1489	Analysis	1482:1489	Analysis of pH during heating and holding at 60 °C	1482:1531	Analysis of pH during heating and holding at 60 °C indicated that SMUF acidified continuously under the period of study, while acidification in BCC/SMUF mixtures terminated after a short period, indicating that the type of CaP formed on heating is altered in the presence of BCC.					
30472620	9	11	theme	temperature-dependant	1826:1846	arg1	changes					1848:1854	pronounced temperature-dependant changes	1815:1854	pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations	1815:1984	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	4	12	theme	different	717:725	arg1	temperatures					727:738	different temperatures	717:738	different temperatures from 4 to 63 °C	717:754	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	5	13	from	lactose	850:856	arg1	thermo-reversible					812:828	thermo-reversible	812:828	thermo-reversible	812:828	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	2	14	theme	present	308:314	arg1	study					316:320	The present study	304:320	The present study	304:320	The present study investigated self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration.					
30472620	1	15	theme	temperature	291:301	arg1	conditions					232:241	appropriate conditions	220:241	appropriate conditions of protein concentration, serum composition and temperature	220:301	Monomeric bovine β-casein self-associates into micelles under appropriate conditions of protein concentration, serum composition and temperature.					
30472620	6	16	theme	residual	1210:1217	arg1	turbidity					1219:1227	residual turbidity	1210:1227	residual turbidity	1210:1227	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	8	17	from	60 °C	1527:1531	arg1	heating					1504:1510	heating	1504:1510	heating	1504:1510	Analysis of pH during heating and holding at 60 °C indicated that SMUF acidified continuously under the period of study, while acidification in BCC/SMUF mixtures terminated after a short period, indicating that the type of CaP formed on heating is altered in the presence of BCC.					
30472620	8	17	from	60 °C	1527:1531	arg1	holding					1516:1522	holding	1516:1522	holding	1516:1522	Analysis of pH during heating and holding at 60 °C indicated that SMUF acidified continuously under the period of study, while acidification in BCC/SMUF mixtures terminated after a short period, indicating that the type of CaP formed on heating is altered in the presence of BCC.					
30472620	4	18	dep	63 °C	750:754	arg1	to					747:748	to	747:748	to	747:748	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	1	19	theme	bovine	168:173	arg1	β-casein					175:182	Monomeric bovine β-casein	158:182	Monomeric bovine β-casein	158:182	Monomeric bovine β-casein self-associates into micelles under appropriate conditions of protein concentration, serum composition and temperature.					
30472620	8	20	theme	CaP	1705:1707	arg1	type					1697:1700	the type	1693:1700	the type of CaP formed on heating	1693:1725	Analysis of pH during heating and holding at 60 °C indicated that SMUF acidified continuously under the period of study, while acidification in BCC/SMUF mixtures terminated after a short period, indicating that the type of CaP formed on heating is altered in the presence of BCC.					
30472620	4	21	from	level	641:645	arg1	formula					657:663	infant formula	650:663	infant formula	650:663	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	3	22	theme	casein	475:480	arg1	ratio					495:499	a casein:whey protein ratio	473:499	a casein:whey protein ratio of 77:23	473:508	The BCC had a casein:whey protein ratio of 77:23, with ∼95% of casein consisting of β-casein, and the remainder being mostly κ-CN.					
30472620	5	23	from	Self-association	768:783	arg1	heating					800:806	heating	800:806	heating	800:806	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	6	24	theme	calcium	1127:1133	arg1	phosphate					1135:1143	calcium phosphate	1127:1143	calcium phosphate (CaP)	1127:1149	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	6	24	theme	calcium	1127:1133	arg1	CaP					1146:1148	CaP	1146:1148	CaP	1146:1148	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	8	25	theme	short	1663:1667	arg1	period					1669:1674	a short period	1661:1674	a short period	1661:1674	Analysis of pH during heating and holding at 60 °C indicated that SMUF acidified continuously under the period of study, while acidification in BCC/SMUF mixtures terminated after a short period, indicating that the type of CaP formed on heating is altered in the presence of BCC.					
30472620	4	26	theme	infant	650:655	arg1	formula					657:663	infant formula	650:663	infant formula	650:663	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	0	27	theme	Colloidal	0:8	arg1	properties					10:19	Colloidal properties	0:19	Colloidal properties of protein complexes formed in β-casein	0:59	Colloidal properties of protein complexes formed in β-casein concentrate solutions as influenced by heating and cooling in the presence of different solutes.					
30472620	9	28	theme	minerals	1926:1933	arg1	presence					1914:1921	the presence	1910:1921	the presence of minerals commonly found in nutritional product formulations	1910:1984	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	5	29	theme	deionised	833:841	arg1	water					843:847	deionised water	833:847	deionised water	833:847	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	9	30	theme	product	1965:1971	arg1	formulations					1973:1984	nutritional product formulations	1953:1984	nutritional product formulations	1953:1984	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	0	31	theme	protein	24:30	arg1	complexes					32:40	protein complexes	24:40	protein complexes formed in β-casein	24:59	Colloidal properties of protein complexes formed in β-casein concentrate solutions as influenced by heating and cooling in the presence of different solutes.					
30472620	2	32	theme	self-association	335:350	arg1	characteristics					352:366	self-association characteristics	335:366	self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration	335:458	The present study investigated self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration.					
30472620	9	33	theme	BCC	1791:1793	arg1	ingredients					1795:1805	BCC ingredients	1791:1805	BCC ingredients	1791:1805	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	3	34	theme	protein	487:493	arg1	ratio					495:499	a casein:whey protein ratio	473:499	a casein:whey protein ratio of 77:23	473:508	The BCC had a casein:whey protein ratio of 77:23, with ∼95% of casein consisting of β-casein, and the remainder being mostly κ-CN.					
30472620	5	35	theme	β-casein	788:795	arg1	Self-association					768:783	Self-association	768:783	Self-association of β-casein on heating	768:806	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	6	36	theme	simulated	1033:1041	arg1	ultrafiltrate					1048:1060	simulated milk ultrafiltrate	1033:1060	simulated milk ultrafiltrate (SMUF)	1033:1067	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	6	36	theme	simulated	1033:1041	arg1	SMUF					1063:1066	SMUF	1063:1066	SMUF	1063:1066	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	4	37	theme	%	620:620	arg1	protein					622:628	1.2% protein	617:628	1.2% protein (a typical level in infant formula)	617:664	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	4	38	theme	liquid	677:682	arg1	media					684:688	various liquid media	669:688	various liquid media at pH 6.8	669:698	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	7	39	theme	turbidity	1400:1408	arg1	development					1410:1420	this turbidity development	1395:1420	this turbidity development	1395:1420	The complexes responsible for this turbidity development were successfully dissociated with 50 mM trisodium citrate.					
30472620	5	40	dep	water	843:847	arg1	solutions					889:897	solutions	889:897	solutions	889:897	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	6	41	theme	BCC	1329:1331	arg1	solutions					1333:1341	BCC solutions	1329:1341	BCC solutions	1329:1341	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	1	42	theme	appropriate	220:230	arg1	conditions					232:241	appropriate conditions	220:241	appropriate conditions of protein concentration, serum composition and temperature	220:301	Monomeric bovine β-casein self-associates into micelles under appropriate conditions of protein concentration, serum composition and temperature.					
30472620	0	43	theme	complexes	32:40	arg1	properties					10:19	Colloidal properties	0:19	Colloidal properties of protein complexes formed in β-casein	0:59	Colloidal properties of protein complexes formed in β-casein concentrate solutions as influenced by heating and cooling in the presence of different solutes.					
30472620	6	44	contain	has	1076:1078	arg1	ultrafiltrate					1048:1060	simulated milk ultrafiltrate	1033:1060	simulated milk ultrafiltrate (SMUF)	1033:1067	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	6	44	contain	has	1076:1078	arg2	strength					1093:1100	a high ionic strength	1080:1100	a high ionic strength	1080:1100	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	6	44	contain	has	1076:1078	arg1	SMUF					1063:1066	SMUF	1063:1066	SMUF	1063:1066	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	6	45	theme	serum	908:912	arg1	phases					914:919	most serum phases	903:919	most serum phases	903:919	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	7	46	theme	responsible	1379:1389	arg1	complexes					1369:1377	The complexes	1365:1377	The complexes responsible for this turbidity development	1365:1420	The complexes responsible for this turbidity development were successfully dissociated with 50 mM trisodium citrate.					
30472620	4	47	theme	1.2	617:619	arg1	%					620:620	%	620:620	%	620:620	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	9	48	theme	pronounced	1815:1824	arg1	changes					1848:1854	pronounced temperature-dependant changes	1815:1854	pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations	1815:1984	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	3	49	theme	whey	482:485	arg1	ratio					495:499	a casein:whey protein ratio	473:499	a casein:whey protein ratio of 77:23	473:508	The BCC had a casein:whey protein ratio of 77:23, with ∼95% of casein consisting of β-casein, and the remainder being mostly κ-CN.					
30472620	9	50	theme	nutritional	1953:1963	arg1	formulations					1973:1984	nutritional product formulations	1953:1984	nutritional product formulations	1953:1984	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	0	51	theme	solutes	149:155	arg1	presence					127:134	the presence	123:134	the presence of different solutes	123:155	Colloidal properties of protein complexes formed in β-casein concentrate solutions as influenced by heating and cooling in the presence of different solutes.					
30472620	9	52	located	found	1944:1948	arg2	minerals					1926:1933	minerals	1926:1933	minerals commonly found in nutritional product formulations	1926:1984	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	9	52	located	found	1944:1948	arg1	formulations					1973:1984	nutritional product formulations	1953:1984	nutritional product formulations	1953:1984	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	4	53	dep	protein	622:628	arg1	level					641:645	a typical level	631:645	a typical level in infant formula	631:663	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	5	54	from	thermo-reversible	812:828	arg1	calcium					874:880	calcium	874:880	calcium (9 mM)	874:887	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	5	54	from	thermo-reversible	812:828	arg1	lactose					850:856	lactose	850:856	lactose (4, 6 or 8%)	850:869	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	5	54	from	thermo-reversible	812:828	arg1	water					843:847	deionised water	833:847	deionised water	833:847	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	5	54	from	thermo-reversible	812:828	arg1	9 mM					883:886	9 mM	883:886	9 mM	883:886	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	5	54	from	thermo-reversible	812:828	arg1	%					868:868	4, 6 or 8%	859:868	4, 6 or 8%	859:868	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	0	55	theme	different	139:147	arg1	solutes					149:155	different solutes	139:155	different solutes	139:155	Colloidal properties of protein complexes formed in β-casein concentrate solutions as influenced by heating and cooling in the presence of different solutes.					
30472620	3	56	contain	had	469:471	arg2	ratio					495:499	a casein:whey protein ratio	473:499	a casein:whey protein ratio of 77:23	473:508	The BCC had a casein:whey protein ratio of 77:23, with ∼95% of casein consisting of β-casein, and the remainder being mostly κ-CN.					
30472620	3	56	contain	had	469:471	arg1	BCC					465:467	The BCC	461:467	The BCC	461:467	The BCC had a casein:whey protein ratio of 77:23, with ∼95% of casein consisting of β-casein, and the remainder being mostly κ-CN.					
30472620	2	57	theme	concentrate	382:392	arg1	characteristics					352:366	self-association characteristics	335:366	self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration	335:458	The present study investigated self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration.					
30472620	4	58	from	pH	693:694	arg1	media					684:688	various liquid media	669:688	various liquid media at pH 6.8	669:698	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	9	59	theme	colloidal	1859:1867	arg1	properties					1869:1878	colloidal properties	1859:1878	colloidal properties	1859:1878	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	6	60	theme	ionic	1087:1091	arg1	strength					1093:1100	a high ionic strength	1080:1100	a high ionic strength	1080:1100	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	2	61	from	pilot-scale	422:432	arg1	milk					414:417	milk	414:417	milk at pilot-scale using membrane filtration	414:458	The present study investigated self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration.					
30472620	2	62	theme	β-casein	373:380	arg1	concentrate					382:392	a β-casein concentrate	371:392	a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration	371:458	The present study investigated self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration.					
30472620	2	62	theme	β-casein	373:380	arg1	BCC					395:397	BCC	395:397	BCC	395:397	The present study investigated self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration.					
30472620	6	63	theme	milk	1043:1046	arg1	ultrafiltrate					1048:1060	simulated milk ultrafiltrate	1033:1060	simulated milk ultrafiltrate (SMUF)	1033:1067	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	6	63	theme	milk	1043:1046	arg1	SMUF					1063:1066	SMUF	1063:1066	SMUF	1063:1066	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	7	64	theme	trisodium	1463:1471	arg1	citrate					1473:1479	50 mM trisodium citrate	1457:1479	50 mM trisodium citrate	1457:1479	The complexes responsible for this turbidity development were successfully dissociated with 50 mM trisodium citrate.					
30472620	6	65	theme	high	1082:1085	arg1	strength					1093:1100	a high ionic strength	1080:1100	a high ionic strength	1080:1100	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	5	66	from	water	843:847	arg1	thermo-reversible					812:828	thermo-reversible	812:828	thermo-reversible	812:828	Self-association of β-casein on heating was thermo-reversible in deionised water, lactose (4, 6 or 8%) or calcium (9 mM) solutions.					
30472620	3	67	theme	77:23	504:508	arg1	ratio					495:499	a casein:whey protein ratio	473:499	a casein:whey protein ratio of 77:23	473:508	The BCC had a casein:whey protein ratio of 77:23, with ∼95% of casein consisting of β-casein, and the remainder being mostly κ-CN.					
30472620	6	68	from	63 °C	967:971	arg1	incubation					953:962	incubation	953:962	incubation at 63 °C	953:971	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	1	69	theme	protein	246:252	arg1	concentration					254:266	protein concentration	246:266	protein concentration	246:266	Monomeric bovine β-casein self-associates into micelles under appropriate conditions of protein concentration, serum composition and temperature.					
30472620	2	70	theme	membrane	440:447	arg1	filtration					449:458	membrane filtration	440:458	membrane filtration	440:458	The present study investigated self-association characteristics of a β-casein concentrate (BCC) prepared from milk at pilot-scale using membrane filtration.					
30472620	9	71	from	changes	1848:1854	arg1	properties					1869:1878	colloidal properties	1859:1878	colloidal properties	1859:1878	This study demonstrates that BCC ingredients exhibit pronounced temperature-dependant changes in colloidal properties that are strongly affected by the presence of minerals commonly found in nutritional product formulations.					
30472620	4	72	theme	typical	633:639	arg1	level					641:645	a typical level	631:645	a typical level in infant formula	631:663	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	1	73	theme	concentration	254:266	arg1	conditions					232:241	appropriate conditions	220:241	appropriate conditions of protein concentration, serum composition and temperature	220:301	Monomeric bovine β-casein self-associates into micelles under appropriate conditions of protein concentration, serum composition and temperature.					
30472620	6	74	from	development	1314:1324	arg1	solutions					1333:1341	BCC solutions	1329:1341	BCC solutions	1329:1341	In most serum phases, BCC became highly opaque after incubation at 63 °C, but clarified rapidly during cooling to 25 °C. However, in simulated milk ultrafiltrate (SMUF), which has a high ionic strength and is supersaturated in calcium phosphate (CaP), BCC remained opaque during cooling to 25 °C, and retained residual turbidity after 15 h of holding at 4 °C; if SMUF was prepared without phosphate then turbidity development in BCC solutions was markedly reduced.					
30472620	4	75	from	63 °C	750:754	arg1	temperatures					727:738	different temperatures	717:738	different temperatures from 4 to 63 °C	717:754	BCC was reconstituted to 1.2% protein (a typical level in infant formula) in various liquid media at pH 6.8 and incubated at different temperatures from 4 to 63 °C for 30 min.					
30472620	3	76	theme	casein	524:529	arg1	%					519:519	∼95%	516:519	∼95% of casein consisting of β-casein	516:552	The BCC had a casein:whey protein ratio of 77:23, with ∼95% of casein consisting of β-casein, and the remainder being mostly κ-CN.					
30472620	3	76	theme	casein	524:529	arg1	casein					524:529	casein	524:529	casein consisting of β-casein	524:552	The BCC had a casein:whey protein ratio of 77:23, with ∼95% of casein consisting of β-casein, and the remainder being mostly κ-CN.					
30472620	8	77	theme	BCC/SMUF	1626:1633	arg1	mixtures					1635:1642	BCC/SMUF mixtures	1626:1642	BCC/SMUF mixtures	1626:1642	Analysis of pH during heating and holding at 60 °C indicated that SMUF acidified continuously under the period of study, while acidification in BCC/SMUF mixtures terminated after a short period, indicating that the type of CaP formed on heating is altered in the presence of BCC.					
30472620	7	78	theme	50 mM	1457:1461	arg1	citrate					1473:1479	50 mM trisodium citrate	1457:1479	50 mM trisodium citrate	1457:1479	The complexes responsible for this turbidity development were successfully dissociated with 50 mM trisodium citrate.					
31915687	9	0	theme	wheat	1543:1547	arg1	bran					1549:1552	wheat bran	1543:1552	wheat bran	1543:1552	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	1	1	theme	microbial	273:281	arg1	lipids					283:288	microbial lipids	273:288	microbial lipids	273:288	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	4	2	theme	oleic	724:728	arg1	acid					730:733	oleic acid	724:733	oleic acid	724:733	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
31915687	10	3	theme	biodiesel	1659:1667	arg1	production					1669:1678	biodiesel production	1659:1678	biodiesel production	1659:1678	The predictive determination of biodiesel properties suggests that this oil may effectively be used for biodiesel production.					
31915687	0	4	theme	Hydrolysate	31:41	arg1	Utilization					0:10	Utilization	0:10	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.	0:144	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.					
31915687	3	5	theme	substrates	540:549	arg1	sucrose					604:610	sucrose	604:610	sucrose	604:610	This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose.					
31915687	3	5	theme	substrates	540:549	arg1	glycerol					591:598	glycerol	591:598	glycerol	591:598	This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose.					
31915687	3	5	theme	substrates	540:549	arg1	range					531:535	a wide range	524:535	a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose	524:610	This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose.					
31915687	9	6	theme	fatty	1391:1395	arg1	composition					1402:1412	The relative fatty acid composition	1378:1412	The relative fatty acid composition	1378:1412	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	6	7	theme	sugars	966:971	arg1	yield					948:952	The highest yield	936:952	The highest yield of reducing sugars (56.6 g/L)	936:982	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	7	8	theme	Hydrolysate	1048:1058	arg1	step					1075:1078	Hydrolysate detoxification step	1048:1078	Hydrolysate detoxification step	1048:1078	Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements.					
31915687	5	9	theme	diluted	795:801	arg1	treatment					808:816	diluted acid treatment	795:816	diluted acid treatment	795:816	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	1	10	theme	mucilaginosa	232:243	arg1	Y-MG1					245:249	Rhodotorula mucilaginosa Y-MG1	220:249	Rhodotorula mucilaginosa Y-MG1	220:249	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	6	11	theme	highest	940:946	arg1	yield					948:952	The highest yield	936:952	The highest yield of reducing sugars (56.6 g/L)	936:982	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	5	12	theme	characterized	898:910	arg1	wastes					928:933	different characterized lignocellulosic wastes	888:933	different characterized lignocellulosic wastes	888:933	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	8	13	theme	fed-batch	1272:1280	arg1	fermentation					1282:1293	controlled fed-batch fermentation	1261:1293	controlled fed-batch fermentation	1261:1293	Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively.					
31915687	9	14	theme	levels	1447:1452	arg1	presence					1425:1432	the presence	1421:1432	the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran	1421:1552	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	6	15	theme	wheat	1007:1011	arg1	material					1038:1045	the raw material	1030:1045	the raw material	1030:1045	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	6	15	theme	wheat	1007:1011	arg1	bran					1013:1016	wheat bran	1007:1016	wheat bran	1007:1016	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	7	16	theme	inhibitors	1190:1199	arg1	presence					1178:1185	the presence	1174:1185	the presence of inhibitors	1174:1199	Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements.					
31915687	5	17	theme	acid	803:806	arg1	treatment					808:816	diluted acid treatment	795:816	diluted acid treatment	795:816	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	9	18	theme	monounsaturated	1457:1471	arg1	acids					1509:1513	monounsaturated (66.8%) and saturated (23.4%) fatty acids	1457:1513	monounsaturated (66.8%) and saturated (23.4%) fatty acids	1457:1513	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	4	19	theme	carbon	687:692	arg1	source					694:699	used carbon source	682:699	used carbon source with a predominance of oleic acid or linoleic acid	682:750	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
31915687	4	20	theme	acid	747:750	arg1	predominance					708:719	a predominance	706:719	a predominance of oleic acid or linoleic acid	706:750	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
31915687	4	21	theme	acid	730:733	arg1	predominance					708:719	a predominance	706:719	a predominance of oleic acid or linoleic acid	706:750	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
31915687	0	22	theme	Lipid	91:95	arg1	Production					97:106	Microbial Lipid Production	81:106	Microbial Lipid Production	81:106	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.					
31915687	5	23	theme	effective	756:764	arg1	process					777:783	An effective hydrolysis process	753:783	An effective hydrolysis process	753:783	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	9	24	theme	saturated	1485:1493	arg1	acids					1509:1513	monounsaturated (66.8%) and saturated (23.4%) fatty acids	1457:1513	monounsaturated (66.8%) and saturated (23.4%) fatty acids	1457:1513	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	8	25	theme	up	1334:1335	arg1	%					1355:1355	38.7%	1351:1355	38.7% (w/w)	1351:1361	Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively.					
31915687	8	25	theme	up	1334:1335	arg1	up					1334:1335	up	1334:1335	up to 11 g/L	1334:1345	Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively.					
31915687	8	25	theme	up	1334:1335	arg1	biomass					1309:1315	a dry biomass and oil yield	1303:1329	biomass	1309:1315	Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively.					
31915687	8	25	theme	up	1334:1335	arg1	yield					1325:1329	a dry biomass and oil yield	1303:1329	yield	1325:1329	Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively.					
31915687	8	25	theme	up	1334:1335	arg1	w/w					1358:1360	w/w	1358:1360	w/w	1358:1360	Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively.					
31915687	2	26	theme	Y-MG1	407:411	arg1	strain					413:418	the Y-MG1 strain	403:418	the Y-MG1 strain	403:418	On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content.					
31915687	6	27	theme	raw	1034:1036	arg1	material					1038:1045	the raw material	1030:1045	the raw material	1030:1045	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	6	27	theme	raw	1034:1036	arg1	bran					1013:1016	wheat bran	1007:1016	wheat bran	1007:1016	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	10	28	theme	biodiesel	1587:1595	arg1	properties					1597:1606	biodiesel properties	1587:1606	biodiesel properties	1587:1606	The predictive determination of biodiesel properties suggests that this oil may effectively be used for biodiesel production.					
31915687	0	29	theme	Wheat	15:19	arg1	Bran					21:24	Wheat Bran	15:24	Wheat Bran Acid Hydrolysate	15:41	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.					
31915687	1	30	theme	raw	307:309	arg1	material					311:318	the potential raw material	293:318	the potential raw material for biodiesel synthesis	293:342	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	1	30	theme	raw	307:309	arg1	hydrolysate					166:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	0	31	theme	Acid	26:29	arg1	Hydrolysate					31:41	Wheat Bran Acid Hydrolysate	15:41	Wheat Bran Acid Hydrolysate	15:41	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.					
31915687	5	32	theme	hydrolysis	766:775	arg1	process					777:783	An effective hydrolysis process	753:783	An effective hydrolysis process	753:783	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	9	33	theme	acids	1509:1513	arg1	levels					1447:1452	increased levels	1437:1452	increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids	1437:1513	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	2	34	theme	content	480:486	arg1	%					469:469	32.7%	465:469	32.7% of lipid content	465:486	On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content.					
31915687	2	34	theme	content	480:486	arg1	content					480:486	lipid content	474:486	lipid content	474:486	On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content.					
31915687	0	35	theme	mucilaginosa	58:69	arg1	Y-MG1					71:75	Rhodotorula mucilaginosa Y-MG1	46:75	Rhodotorula mucilaginosa Y-MG1	46:75	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.					
31915687	2	36	theme	synthetic	348:356	arg1	media					358:362	synthetic media	348:362	synthetic media	348:362	On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content.					
31915687	1	37	theme	Y-MG1	245:249	arg1	hydrolysate					166:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	1	37	theme	Y-MG1	245:249	arg1	feedstock					207:215	the fermentation feedstock	190:215	the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids	190:288	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	9	38	theme	Y-MG1	1528:1532	arg1	lipids					1518:1523	lipids	1518:1523	lipids of Y-MG1 grown on wheat bran	1518:1552	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	7	39	theme	external	1229:1236	arg1	elements					1238:1245	external elements	1229:1245	external elements	1229:1245	Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements.					
31915687	3	40	theme	wide	526:529	arg1	range					531:535	a wide range	524:535	a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose	524:610	This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose.					
31915687	2	41	theme	nitrogen-limiting	374:390	arg1	condition					392:400	nitrogen-limiting condition	374:400	nitrogen-limiting condition	374:400	On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content.					
31915687	4	42	with	source	694:699	arg1	predominance					708:719	a predominance	706:719	a predominance of oleic acid or linoleic acid	706:750	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
31915687	1	43	theme	lipids	283:288	arg1	production					259:268	the production	255:268	the production of microbial lipids	255:288	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	8	44	theme	dry	1305:1307	arg1	biomass					1309:1315	a dry biomass and oil yield	1303:1329	biomass	1309:1315	Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively.					
31915687	7	45	theme	detoxification	1060:1073	arg1	step					1075:1078	Hydrolysate detoxification step	1048:1078	Hydrolysate detoxification step	1048:1078	Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements.					
31915687	3	46	theme	C6	570:571	arg1	substrates					540:549	substrates	540:549	substrates	540:549	This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose.					
31915687	3	46	theme	C6	570:571	arg1	sugars					573:578	C6 sugars	570:578	C6 sugars	570:578	This strain was able to assimilate a wide range of substrates, especially C5 and C6 sugars as well as glycerol and sucrose.					
31915687	9	47	theme	relative	1382:1389	arg1	composition					1402:1412	The relative fatty acid composition	1378:1412	The relative fatty acid composition	1378:1412	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	9	48	from	presence	1425:1432	arg1	lipids					1518:1523	lipids	1518:1523	lipids of Y-MG1 grown on wheat bran	1518:1552	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	0	49	theme	Biodiesel	125:133	arg1	Synthesis					135:143	Biodiesel Synthesis	125:143	Biodiesel Synthesis	125:143	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.					
31915687	9	50	theme	acid	1397:1400	arg1	composition					1402:1412	The relative fatty acid composition	1378:1412	The relative fatty acid composition	1378:1412	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	6	51	theme	reducing	957:964	arg1	56.6 g/L					974:981	56.6 g/L	974:981	56.6 g/L	974:981	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	6	51	theme	reducing	957:964	arg1	sugars					966:971	reducing sugars	957:971	reducing sugars (56.6 g/L)	957:982	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	4	52	theme	acid	619:622	arg1	composition					624:634	Fatty acid composition	613:634	Fatty acid composition	613:634	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
31915687	1	53	theme	fermentation	194:205	arg1	hydrolysate					166:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	1	53	theme	fermentation	194:205	arg1	feedstock					207:215	the fermentation feedstock	190:215	the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids	190:288	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	1	54	theme	Rhodotorula	220:230	arg1	Y-MG1					245:249	Rhodotorula mucilaginosa Y-MG1	220:249	Rhodotorula mucilaginosa Y-MG1	220:249	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	7	55	from	lipids	1164:1169	arg1	presence					1178:1185	the presence	1174:1185	the presence of inhibitors	1174:1199	Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements.					
31915687	5	56	theme	different	888:896	arg1	wastes					928:933	different characterized lignocellulosic wastes	888:933	different characterized lignocellulosic wastes	888:933	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	4	57	theme	source	694:699	arg1	nature					672:677	the nature	668:677	the nature of used carbon source with a predominance of oleic acid or linoleic acid	668:750	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
31915687	9	58	theme	increased	1437:1445	arg1	levels					1447:1452	increased levels	1437:1452	increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids	1437:1513	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	8	59	theme	controlled	1261:1270	arg1	fermentation					1282:1293	controlled fed-batch fermentation	1261:1293	controlled fed-batch fermentation	1261:1293	Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively.					
31915687	10	60	used	used	1650:1653	arg2	oil					1627:1629	this oil	1622:1629	this oil	1622:1629	The predictive determination of biodiesel properties suggests that this oil may effectively be used for biodiesel production.					
31915687	5	61	theme	fermentable	864:874	arg1	sugars					876:881	fermentable sugars	864:881	fermentable sugars from different characterized lignocellulosic wastes	864:933	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	1	62	used	used	182:185	arg2	hydrolysate					166:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	1	62	used	used	182:185	arg2	material					311:318	the potential raw material	293:318	the potential raw material for biodiesel synthesis	293:342	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	1	62	used	used	182:185	arg2	feedstock					207:215	the fermentation feedstock	190:215	the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids	190:288	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	0	63	theme	Microbial	81:89	arg1	Production					97:106	Microbial Lipid Production	81:106	Microbial Lipid Production	81:106	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.					
31915687	5	64	theme	sugars	876:881	arg1	maximum					853:859	the maximum	849:859	the maximum of fermentable sugars from different characterized lignocellulosic wastes	849:933	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	4	65	theme	used	682:685	arg1	source					694:699	used carbon source	682:699	used carbon source with a predominance of oleic acid or linoleic acid	682:750	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
31915687	6	66	used	used	1022:1025	arg2	bran					1013:1016	wheat bran	1007:1016	wheat bran	1007:1016	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	6	66	used	used	1022:1025	arg2	material					1038:1045	the raw material	1030:1045	the raw material	1030:1045	The highest yield of reducing sugars (56.6 g/L) could be achieved when wheat bran was used as the raw material.					
31915687	4	67	theme	linoleic	738:745	arg1	acid					747:750	linoleic acid	738:750	linoleic acid	738:750	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
31915687	5	68	theme	lignocellulosic	912:926	arg1	wastes					928:933	different characterized lignocellulosic wastes	888:933	different characterized lignocellulosic wastes	888:933	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	8	69	theme	oil	1321:1323	arg1	yield					1325:1329	a dry biomass and oil yield	1303:1329	yield	1325:1329	Operating by controlled fed-batch fermentation yielded a dry biomass and oil yield of up to 11 g/L and 38.7% (w/w), respectively.					
31915687	5	70	from	wastes	928:933	arg1	sugars					876:881	fermentable sugars	864:881	fermentable sugars from different characterized lignocellulosic wastes	864:933	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	5	70	from	wastes	928:933	arg1	maximum					853:859	the maximum	849:859	the maximum of fermentable sugars from different characterized lignocellulosic wastes	849:933	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	0	71	theme	Bran	21:24	arg1	Hydrolysate					31:41	Wheat Bran Acid Hydrolysate	15:41	Wheat Bran Acid Hydrolysate	15:41	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.					
31915687	1	72	theme	potential	297:305	arg1	material					311:318	the potential raw material	293:318	the potential raw material for biodiesel synthesis	293:342	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	1	72	theme	potential	297:305	arg1	hydrolysate					166:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	9	73	attach	presence	1425:1432	arg1	lipids					1518:1523	lipids	1518:1523	lipids of Y-MG1 grown on wheat bran	1518:1552	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	9	73	attach	presence	1425:1432	arg2	levels					1447:1452	increased levels	1437:1452	increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids	1437:1513	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	9	74	dep	monounsaturated	1457:1471	arg1	%					1478:1478	66.8%	1474:1478	66.8%	1474:1478	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	9	74	dep	monounsaturated	1457:1471	arg1	%					1500:1500	23.4%	1496:1500	23.4%	1496:1500	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	2	75	theme	lipids	441:446	arg1	2.13 g/L					429:436	2.13 g/L	429:436	2.13 g/L of lipids corresponding to 32.7% of lipid content	429:486	On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content.					
31915687	7	76	theme	Y-MG1	1121:1125	arg1	strain					1127:1132	the Y-MG1 strain	1117:1132	the Y-MG1 strain	1117:1132	Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements.					
31915687	9	77	theme	fatty	1503:1507	arg1	acids					1509:1513	monounsaturated (66.8%) and saturated (23.4%) fatty acids	1457:1513	monounsaturated (66.8%) and saturated (23.4%) fatty acids	1457:1513	The relative fatty acid composition showed the presence of increased levels of monounsaturated (66.8%) and saturated (23.4%) fatty acids in lipids of Y-MG1 grown on wheat bran.					
31915687	10	78	theme	predictive	1559:1568	arg1	determination					1570:1582	The predictive determination	1555:1582	The predictive determination of biodiesel properties	1555:1606	The predictive determination of biodiesel properties suggests that this oil may effectively be used for biodiesel production.					
31915687	0	79	theme	Rhodotorula	46:56	arg1	Y-MG1					71:75	Rhodotorula mucilaginosa Y-MG1	46:75	Rhodotorula mucilaginosa Y-MG1	46:75	Utilization of Wheat Bran Acid Hydrolysate by Rhodotorula mucilaginosa Y-MG1 for Microbial Lipid Production as Feedstock for Biodiesel Synthesis.					
31915687	1	80	theme	biodiesel	324:332	arg1	synthesis					334:342	biodiesel synthesis	324:342	biodiesel synthesis	324:342	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	5	81	from	maximum	853:859	arg1	wastes					928:933	different characterized lignocellulosic wastes	888:933	different characterized lignocellulosic wastes	888:933	An effective hydrolysis process, based on diluted acid treatment, was established for providing the maximum of fermentable sugars from different characterized lignocellulosic wastes.					
31915687	7	82	theme	elements	1238:1245	arg1	addition					1217:1224	the addition	1213:1224	the addition of external elements	1213:1245	Hydrolysate detoxification step was not required in this study since the Y-MG1 strain was shown to grow and produce lipids in the presence of inhibitors and without the addition of external elements.					
31915687	2	83	theme	lipid	474:478	arg1	content					480:486	lipid content	474:486	lipid content	474:486	On synthetic media and under nitrogen-limiting condition, the Y-MG1 strain produces 2.13 g/L of lipids corresponding to 32.7% of lipid content.					
31915687	1	84	theme	lignocellulosic	150:164	arg1	material					311:318	the potential raw material	293:318	the potential raw material for biodiesel synthesis	293:342	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	1	84	theme	lignocellulosic	150:164	arg1	hydrolysate					166:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate	146:176	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	1	84	theme	lignocellulosic	150:164	arg1	feedstock					207:215	the fermentation feedstock	190:215	the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids	190:288	The lignocellulosic hydrolysate was used as the fermentation feedstock of Rhodotorula mucilaginosa Y-MG1 for the production of microbial lipids as the potential raw material for biodiesel synthesis.					
31915687	10	85	theme	properties	1597:1606	arg1	determination					1570:1582	The predictive determination	1555:1582	The predictive determination of biodiesel properties	1555:1606	The predictive determination of biodiesel properties suggests that this oil may effectively be used for biodiesel production.					
31915687	4	86	theme	Fatty	613:617	arg1	composition					624:634	Fatty acid composition	613:634	Fatty acid composition	613:634	Fatty acid composition shows a divergence depending on the nature of used carbon source with a predominance of oleic acid or linoleic acid.					
29701745	4	0	theme	electrochemical	466:480	arg1	spectroscopy					492:503	electrochemical impedance spectroscopy	466:503	electrochemical impedance spectroscopy as well as neutron scattering techniques	466:544	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	5	1	theme	bilayer	842:848	arg1	interaction					823:833	the interaction	819:833	the interaction of the bilayer with the antibiotic colistin	819:877	The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin.					
29701745	5	2	from	influence	739:747	arg1	stability					782:790	the membrane stability	769:790	the membrane stability	769:790	The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin.					
29701745	4	3	theme	membrane	657:664	arg1	leaflet					666:672	the outer membrane leaflet	647:672	the outer membrane leaflet	647:672	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	6	4	theme	drug	1045:1048	arg1	candidates					1050:1059	potential drug candidates	1035:1059	potential drug candidates	1035:1059	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	4	5	theme	spectroscopy	492:503	arg1	techniques					535:544	electrochemical impedance spectroscopy as well as neutron scattering techniques	466:544	electrochemical impedance spectroscopy as well as neutron scattering techniques	466:544	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	2	6	theme	lipopolysaccharide	263:280	arg1	molecules					282:290	lipopolysaccharide molecules	263:290	lipopolysaccharide molecules	263:290	The structure is based on a tethered monolayer which has been fused with vesicles containing lipopolysaccharide molecules.					
29701745	4	7	theme	diluting	628:635	arg1	lipids					637:642	diluting lipids	628:642	diluting lipids	628:642	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	3	8	from	lipids	348:353	arg1	layer					368:372	the outer layer	358:372	the outer layer	358:372	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	4	9	theme	supported	715:723	arg1	membrane					725:732	a stable solid supported membrane	700:732	a stable solid supported membrane	700:732	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	6	10	theme	membrane	970:977	arg1	proteins					979:986	bacterial membrane proteins	960:986	bacterial membrane proteins	960:986	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	6	11	theme	antimicrobial	1009:1021	arg1	activity					1023:1030	antimicrobial activity	1009:1030	antimicrobial activity of potential drug candidates	1009:1059	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	1	12	theme	Gram	146:149	arg1	bacteria					160:167	Gram negative bacteria	146:167	Gram negative bacteria	146:167	A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria.					
29701745	3	13	theme	monolayer	330:338	arg1	composition					311:321	the composition	307:321	the composition of the monolayer	307:338	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	3	13	theme	monolayer	330:338	arg1	lipids					348:353	the lipids	344:353	the lipids in the outer layer	344:372	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	1	14	theme	model	69:73	arg1	system					84:89	A model membrane system	67:89	A model membrane system	67:89	A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria.					
29701745	6	15	theme	potential	1035:1043	arg1	candidates					1050:1059	potential drug candidates	1035:1059	potential drug candidates	1035:1059	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	1	16	theme	negative	151:158	arg1	bacteria					160:167	Gram negative bacteria	146:167	Gram negative bacteria	146:167	A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria.					
29701745	0	17	theme	tethered	2:9	arg1	membrane					25:32	A tethered bilayer lipid membrane	0:32	A tethered bilayer lipid membrane that mimics microbial membranes	0:64	A tethered bilayer lipid membrane that mimics microbial membranes.					
29701745	4	18	from	density	598:604	arg1	leaflet					666:672	the outer membrane leaflet	647:672	the outer membrane leaflet	647:672	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	1	19	theme	membrane	75:82	arg1	system					84:89	A model membrane system	67:89	A model membrane system	67:89	A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria.					
29701745	5	20	with	interaction	823:833	arg1	colistin					870:877	the antibiotic colistin	855:877	the antibiotic colistin	855:877	The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin.					
29701745	1	21	theme	bacteria	160:167	arg1	membrane					134:141	the outer membrane	124:141	the outer membrane of Gram negative bacteria	124:167	A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria.					
29701745	3	22	theme	outer	362:366	arg1	layer					368:372	the outer layer	358:372	the outer layer	358:372	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	0	23	theme	lipid	19:23	arg1	membrane					25:32	A tethered bilayer lipid membrane	0:32	A tethered bilayer lipid membrane that mimics microbial membranes	0:64	A tethered bilayer lipid membrane that mimics microbial membranes.					
29701745	4	24	from	amount	618:623	arg1	leaflet					666:672	the outer membrane leaflet	647:672	the outer membrane leaflet	647:672	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	3	25	theme	electrical	396:405	arg1	properties					407:416	the structural and electrical properties	377:416	the structural and electrical properties of the membrane	377:432	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	3	26	theme	lipids	348:353	arg1	effect					297:302	The effect	293:302	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane	293:432	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	4	27	theme	solid	709:713	arg1	membrane					725:732	a stable solid supported membrane	700:732	a stable solid supported membrane	700:732	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	0	28	theme	bilayer	11:17	arg1	membrane					25:32	A tethered bilayer lipid membrane	0:32	A tethered bilayer lipid membrane that mimics microbial membranes	0:64	A tethered bilayer lipid membrane that mimics microbial membranes.					
29701745	2	29	contain	containing	252:261	arg1	vesicles					243:250	vesicles	243:250	vesicles containing lipopolysaccharide molecules	243:290	The structure is based on a tethered monolayer which has been fused with vesicles containing lipopolysaccharide molecules.					
29701745	2	29	contain	containing	252:261	arg2	molecules					282:290	lipopolysaccharide molecules	263:290	lipopolysaccharide molecules	263:290	The structure is based on a tethered monolayer which has been fused with vesicles containing lipopolysaccharide molecules.					
29701745	4	30	theme	stable	702:707	arg1	membrane					725:732	a stable solid supported membrane	700:732	a stable solid supported membrane	700:732	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	5	31	theme	membrane	773:780	arg1	stability					782:790	the membrane stability	769:790	the membrane stability	769:790	The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin.					
29701745	6	32	theme	architectures	902:914	arg1	number					882:887	A number	880:887	A number of different architectures	880:914	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	4	33	theme	lipids	637:642	arg1	amount					618:623	a small amount	610:623	a small amount of diluting lipids	610:642	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	4	33	theme	lipids	637:642	arg1	lipids					637:642	diluting lipids	628:642	diluting lipids	628:642	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	4	33	theme	lipids	637:642	arg1	density					598:604	a relatively high tethering density	570:604	a relatively high tethering density	570:604	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	3	34	theme	structural	381:390	arg1	properties					407:416	the structural and electrical properties	377:416	the structural and electrical properties of the membrane	377:432	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	6	35	theme	different	892:900	arg1	architectures					902:914	different architectures	892:914	different architectures	892:914	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	2	36	theme	tethered	198:205	arg1	monolayer					207:215	a tethered monolayer	196:215	a tethered monolayer which has been fused with vesicles containing lipopolysaccharide molecules	196:290	The structure is based on a tethered monolayer which has been fused with vesicles containing lipopolysaccharide molecules.					
29701745	3	37	theme	membrane	425:432	arg1	properties					407:416	the structural and electrical properties	377:416	the structural and electrical properties of the membrane	377:432	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	0	38	theme	microbial	46:54	arg1	membranes					56:64	microbial membranes	46:64	microbial membranes	46:64	A tethered bilayer lipid membrane that mimics microbial membranes.					
29701745	6	39	theme	proteins	979:986	arg1	screening					996:1004	the screening	992:1004	the screening of antimicrobial activity of potential drug candidates	992:1059	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	6	39	theme	proteins	979:986	arg1	study					951:955	the study	947:955	the study of bacterial membrane proteins	947:986	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	5	40	theme	ions	761:764	arg1	influence					739:747	The influence	735:747	The influence of divalent ions on the membrane stability	735:790	The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin.					
29701745	4	41	theme	scattering	524:533	arg1	techniques					535:544	electrochemical impedance spectroscopy as well as neutron scattering techniques	466:544	electrochemical impedance spectroscopy as well as neutron scattering techniques	466:544	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	4	42	theme	impedance	482:490	arg1	spectroscopy					492:503	electrochemical impedance spectroscopy	466:503	electrochemical impedance spectroscopy as well as neutron scattering techniques	466:544	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	5	43	theme	antibiotic	859:868	arg1	colistin					870:877	the antibiotic colistin	855:877	the antibiotic colistin	855:877	The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin.					
29701745	4	44	theme	neutron	516:522	arg1	scattering					524:533	neutron scattering	516:533	electrochemical impedance spectroscopy as well as neutron scattering techniques	466:544	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	4	45	theme	high	583:586	arg1	density					598:604	a relatively high tethering density	570:604	a relatively high tethering density	570:604	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	3	46	from	composition	311:321	arg1	layer					368:372	the outer layer	358:372	the outer layer	358:372	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	4	47	theme	membrane	725:732	arg1	formation					687:695	the formation	683:695	the formation of a stable solid supported membrane	683:732	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	6	48	theme	candidates	1050:1059	arg1	activity					1023:1030	antimicrobial activity	1009:1030	antimicrobial activity of potential drug candidates	1009:1059	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	6	49	theme	bacterial	960:968	arg1	proteins					979:986	bacterial membrane proteins	960:986	bacterial membrane proteins	960:986	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	3	50	theme	composition	311:321	arg1	effect					297:302	The effect	293:302	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane	293:432	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	4	51	theme	small	612:616	arg1	amount					618:623	a small amount	610:623	a small amount of diluting lipids	610:642	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	4	51	theme	small	612:616	arg1	lipids					637:642	diluting lipids	628:642	diluting lipids	628:642	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	4	52	theme	outer	651:655	arg1	leaflet					666:672	the outer membrane leaflet	647:672	the outer membrane leaflet	647:672	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	6	53	theme	activity	1023:1030	arg1	screening					996:1004	the screening	992:1004	the screening of antimicrobial activity of potential drug candidates	992:1059	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	6	53	theme	activity	1023:1030	arg1	study					951:955	the study	947:955	the study of bacterial membrane proteins	947:986	A number of different architectures were developed, suited to both the study of bacterial membrane proteins and the screening of antimicrobial activity of potential drug candidates.					
29701745	5	54	theme	divalent	752:759	arg1	ions					761:764	divalent ions	752:764	divalent ions	752:764	The influence of divalent ions on the membrane stability has been probed as well as the interaction of the bilayer with the antibiotic colistin.					
29701745	3	55	from	layer	368:372	arg1	effect					297:302	The effect	293:302	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane	293:432	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	4	56	theme	tethering	588:596	arg1	density					598:604	a relatively high tethering density	570:604	a relatively high tethering density	570:604	By using electrochemical impedance spectroscopy as well as neutron scattering techniques, it could be shown that a relatively high tethering density and a small amount of diluting lipids in the outer membrane leaflet leads to the formation of a stable solid supported membrane.					
29701745	1	57	theme	outer	128:132	arg1	membrane					134:141	the outer membrane	124:141	the outer membrane of Gram negative bacteria	124:167	A model membrane system has been developed, which mimics the outer membrane of Gram negative bacteria.					
29701745	3	58	from	effect	297:302	arg1	properties					407:416	the structural and electrical properties	377:416	the structural and electrical properties of the membrane	377:432	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
29701745	3	58	from	effect	297:302	arg1	layer					368:372	the outer layer	358:372	the outer layer	358:372	The effect of the composition of the monolayer and the lipids in the outer layer on the structural and electrical properties of the membrane has been investigated.					
31659420	4	0	theme	MiSeq	827:831	arg1	sequencing					849:858	Illumina MiSeq high-throughput sequencing	818:858	Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene	818:906	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	5	1	gly	sialylated	988:997	arg1	groups					1009:1014	the sialylated lactulose groups	984:1014	the sialylated lactulose groups	984:1014	At the phylum level, the relative abundance of Firmicutes was increased in the sialylated lactulose groups, while the abundance of Bacteroidetes was decreased.					
31659420	3	2	theme	Neu5Ac-α2,6-lactulose	643:663	arg1	groups					491:496	six groups	487:496	six groups	487:496	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	3	2	theme	Neu5Ac-α2,6-lactulose	643:663	arg1	group					665:669	a Neu5Ac-α2,6-lactulose group	641:669	a Neu5Ac-α2,6-lactulose group	641:669	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	7	3	theme	lactulose	1424:1432	arg1	groups					1434:1439	the sialylated lactulose groups	1409:1439	the sialylated lactulose groups	1409:1439	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	9	4	with	component	1865:1873	arg1	applications					1902:1913	potential therapeutic applications	1880:1913	potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health	1880:2000	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	3	5	theme	control	516:522	arg1	groups					491:496	six groups	487:496	six groups	487:496	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	3	5	theme	control	516:522	arg1	group					524:528	a normal control group	507:528	a normal control group	507:528	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	1	6	theme	ammonia	273:279	arg1	levels					237:242	the levels	233:242	the levels of blood endotoxin and blood ammonia	233:279	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31659420	7	7	gly	sialylated	1413:1422	arg1	groups					1434:1439	the sialylated lactulose groups	1409:1439	the sialylated lactulose groups	1409:1439	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	7	8	theme	normal	1453:1458	arg1	group					1468:1472	the normal control group	1449:1472	the normal control group	1449:1472	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	9	9	theme	therapeutic	1890:1900	arg1	applications					1902:1913	potential therapeutic applications	1880:1913	potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health	1880:2000	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	9	10	theme	intestinal	1931:1940	arg1	microbiota					1942:1951	intestinal microbiota	1931:1951	intestinal microbiota	1931:1951	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	6	11	gly	sialylated	1296:1305	arg1	lactulose					1307:1315	sialylated lactulose	1296:1315	sialylated lactulose	1296:1315	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	3	12	theme	lactulose	533:541	arg1	groups					491:496	six groups	487:496	six groups	487:496	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	3	12	theme	lactulose	533:541	arg1	group					543:547	a lactulose group	531:547	a lactulose group	531:547	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	7	13	theme	Chao	1390:1393	arg1	higher					1399:1404	higher	1399:1404	higher	1399:1404	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	7	13	theme	Chao	1390:1393	arg1	index					1381:1385	the index	1377:1385	the index of Chao	1377:1393	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	9	14	theme	beneficial	1962:1971	arg1	effects					1973:1979	beneficial effects	1962:1979	beneficial effects	1962:1979	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	6	15	gly	sialylated	1090:1099	arg1	intervention					1111:1122	sialylated lactulose intervention	1090:1122	sialylated lactulose intervention	1090:1122	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	6	16	theme	intestinal	1320:1329	arg1	microbiota					1331:1340	intestinal microbiota	1320:1340	intestinal microbiota	1320:1340	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	8	17	theme	lactulose	1756:1764	arg1	groups					1766:1771	the sialylated lactulose groups	1741:1771	the sialylated lactulose groups	1741:1771	The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.					
31659420	1	18	theme	bifidobacteria	208:221	arg1	level					199:203	the level	195:203	the level of bifidobacteria	195:221	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31659420	4	19	theme	rDNA	898:901	arg1	gene					903:906	the 16S rDNA gene	890:906	the 16S rDNA gene	890:906	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	6	20	theme	lactulose	1307:1315	arg1	effect					1286:1291	the modulatory effect	1271:1291	the modulatory effect of sialylated lactulose on intestinal microbiota	1271:1340	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	3	21	theme	Kdn-α2,3-lactulose	552:569	arg1	groups					491:496	six groups	487:496	six groups	487:496	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	3	21	theme	Kdn-α2,3-lactulose	552:569	arg1	group					571:575	a Kdn-α2,3-lactulose group	550:575	a Kdn-α2,3-lactulose group	550:575	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	7	22	theme	normal	1613:1618	arg1	group					1628:1632	the normal control group	1609:1632	the normal control group	1609:1632	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	6	23	theme	Helicobacteraceae	1190:1206	arg1	abundance					1147:1155	the relative abundance	1134:1155	the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae	1134:1206	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	5	24	theme	sialylated	988:997	arg1	groups					1009:1014	the sialylated lactulose groups	984:1014	the sialylated lactulose groups	984:1014	At the phylum level, the relative abundance of Firmicutes was increased in the sialylated lactulose groups, while the abundance of Bacteroidetes was decreased.					
31659420	8	25	theme	normal	1781:1786	arg1	group					1796:1800	the normal control group	1777:1800	the normal control group	1777:1800	The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.					
31659420	8	26	gly	sialylated	1745:1754	arg1	groups					1766:1771	the sialylated lactulose groups	1741:1771	the sialylated lactulose groups	1741:1771	The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.					
31659420	6	27	theme	group	1180:1184	arg1	abundance					1147:1155	the relative abundance	1134:1155	the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae	1134:1206	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	4	28	theme	lactulose	689:697	arg1	administration					699:712	lactulose administration	689:712	lactulose administration	689:712	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	6	29	theme	modulatory	1275:1284	arg1	effect					1286:1291	the modulatory effect	1271:1291	the modulatory effect of sialylated lactulose on intestinal microbiota	1271:1340	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	3	30	theme	Kdn-α2,6-lactulose	580:597	arg1	groups					491:496	six groups	487:496	six groups	487:496	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	3	30	theme	Kdn-α2,6-lactulose	580:597	arg1	group					599:603	a Kdn-α2,6-lactulose group	578:603	a Kdn-α2,6-lactulose group	578:603	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	6	31	theme	Bacteroidales	1160:1172	arg1	group					1180:1184	Bacteroidales S24-7 group	1160:1184	Bacteroidales S24-7 group	1160:1184	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	4	32	from	group	749:753	arg1	feces					719:723	the feces	715:723	the feces of three mice from each group	715:753	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	0	33	theme	Illumina	73:80	arg1	sequencing					98:107	Illumina high-throughput sequencing	73:107	Illumina high-throughput sequencing	73:107	Effects of sialylated lactulose on the mouse intestinal microbiome using Illumina high-throughput sequencing.					
31659420	9	34	gly	sialylated	1809:1818	arg1	lactulose					1820:1828	sialylated lactulose	1809:1828	sialylated lactulose	1809:1828	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	9	34	gly	sialylated	1809:1818	arg1	component					1865:1873	a functional food component	1847:1873	a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health	1847:2000	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	0	35	from	Effects	0:6	arg1	microbiome					56:65	the mouse intestinal microbiome	35:65	the mouse intestinal microbiome	35:65	Effects of sialylated lactulose on the mouse intestinal microbiome using Illumina high-throughput sequencing.					
31659420	5	36	theme	Bacteroidetes	1040:1052	arg1	abundance					1027:1035	the abundance	1023:1035	the abundance of Bacteroidetes	1023:1052	At the phylum level, the relative abundance of Firmicutes was increased in the sialylated lactulose groups, while the abundance of Bacteroidetes was decreased.					
31659420	3	37	theme	healthy	447:453	arg1	mice					455:458	60 healthy mice	444:458	60 healthy mice	444:458	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	0	38	theme	sialylated	11:20	arg1	lactulose					22:30	sialylated lactulose	11:30	sialylated lactulose	11:30	Effects of sialylated lactulose on the mouse intestinal microbiome using Illumina high-throughput sequencing.					
31659420	3	39	theme	Neu5Ac-α2,3-lactulose	608:628	arg1	groups					491:496	six groups	487:496	six groups	487:496	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	3	39	theme	Neu5Ac-α2,3-lactulose	608:628	arg1	group					630:634	a Neu5Ac-α2,3-lactulose group	606:634	a Neu5Ac-α2,3-lactulose group	606:634	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	6	40	from	effect	1286:1291	arg1	microbiota					1331:1340	intestinal microbiota	1320:1340	intestinal microbiota	1320:1340	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	7	41	theme	diversity	1503:1511	arg1	indices					1513:1519	the Shannon and Simpson diversity indices	1479:1519	the Shannon and Simpson diversity indices	1479:1519	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	7	41	theme	diversity	1503:1511	arg1	higher					1526:1531	higher	1526:1531	higher	1526:1531	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	4	42	theme	intestinal	779:788	arg1	microbiota					790:799	the intestinal microbiota	775:799	the intestinal microbiota	775:799	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	0	43	theme	mouse	39:43	arg1	microbiome					56:65	the mouse intestinal microbiome	35:65	the mouse intestinal microbiome	35:65	Effects of sialylated lactulose on the mouse intestinal microbiome using Illumina high-throughput sequencing.					
31659420	1	44	theme	immune	307:312	arg1	system					314:319	the body's immune system	296:319	the body's immune system	296:319	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31659420	6	45	theme	family	1076:1081	arg1	level					1083:1087	the family level	1072:1087	the family level	1072:1087	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	9	46	theme	functional	1849:1858	arg1	component					1865:1873	a functional food component	1847:1873	a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health	1847:2000	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	9	46	theme	functional	1849:1858	arg1	lactulose					1820:1828	sialylated lactulose	1809:1828	sialylated lactulose	1809:1828	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	2	47	theme	sialylated	353:362	arg1	lactuloses					364:373	sialylated lactuloses	353:373	sialylated lactuloses	353:373	However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota.					
31659420	5	48	theme	Firmicutes	956:965	arg1	abundance					943:951	the relative abundance	930:951	the relative abundance of Firmicutes	930:965	At the phylum level, the relative abundance of Firmicutes was increased in the sialylated lactulose groups, while the abundance of Bacteroidetes was decreased.					
31659420	9	49	theme	potential	1880:1888	arg1	applications					1902:1913	potential therapeutic applications	1880:1913	potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health	1880:2000	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	8	50	theme	microbiota	1665:1674	arg1	composition					1683:1693	the intestinal microbiota sample composition	1650:1693	the intestinal microbiota sample composition	1650:1693	The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.					
31659420	1	51	theme	endotoxin	253:261	arg1	levels					237:242	the levels	233:242	the levels of blood endotoxin and blood ammonia	233:279	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31659420	7	52	theme	control	1460:1466	arg1	group					1468:1472	the normal control group	1449:1472	the normal control group	1449:1472	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	4	53	theme	Illumina	818:825	arg1	sequencing					849:858	Illumina MiSeq high-throughput sequencing	818:858	Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene	818:906	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	3	54	theme	normal	509:514	arg1	groups					491:496	six groups	487:496	six groups	487:496	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	3	54	theme	normal	509:514	arg1	group					524:528	a normal control group	507:528	a normal control group	507:528	In this study, 60 healthy mice were randomly divided into six groups, namely, a normal control group, a lactulose group, a Kdn-α2,3-lactulose group, a Kdn-α2,6-lactulose group, a Neu5Ac-α2,3-lactulose group, and a Neu5Ac-α2,6-lactulose group.					
31659420	8	55	theme	composition	1683:1693	arg1	results					1639:1645	The results	1635:1645	The results of the intestinal microbiota sample composition	1635:1693	The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.					
31659420	1	56	theme	blood	267:271	arg1	ammonia					273:279	blood ammonia	267:279	blood ammonia	267:279	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31659420	4	57	theme	high-throughput	833:847	arg1	sequencing					849:858	Illumina MiSeq high-throughput sequencing	818:858	Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene	818:906	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	6	58	theme	relative	1138:1145	arg1	abundance					1147:1155	the relative abundance	1134:1155	the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae	1134:1206	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	6	59	theme	Lactobacillaceae	1238:1253	arg1	abundance					1225:1233	the abundance	1221:1233	the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota	1221:1340	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	7	60	theme	sialylated	1413:1422	arg1	groups					1434:1439	the sialylated lactulose groups	1409:1439	the sialylated lactulose groups	1409:1439	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	6	61	theme	lactulose	1101:1109	arg1	intervention					1111:1122	sialylated lactulose intervention	1090:1122	sialylated lactulose intervention	1090:1122	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	7	62	theme	control	1620:1626	arg1	group					1628:1632	the normal control group	1609:1632	the normal control group	1609:1632	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	2	63	contain	have	375:378	arg1	lactuloses					364:373	sialylated lactuloses	353:373	sialylated lactuloses	353:373	However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota.					
31659420	2	63	contain	have	375:378	arg2	effects					391:397	modulatory effects	380:397	modulatory effects	380:397	However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota.					
31659420	8	64	theme	sialylated	1745:1754	arg1	groups					1766:1771	the sialylated lactulose groups	1741:1771	the sialylated lactulose groups	1741:1771	The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.					
31659420	4	65	theme	16S	894:896	arg1	gene					903:906	the 16S rDNA gene	890:906	the 16S rDNA gene	890:906	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	6	66	theme	sialylated	1296:1305	arg1	lactulose					1307:1315	sialylated lactulose	1296:1315	sialylated lactulose	1296:1315	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	5	67	theme	lactulose	999:1007	arg1	groups					1009:1014	the sialylated lactulose groups	984:1014	the sialylated lactulose groups	984:1014	At the phylum level, the relative abundance of Firmicutes was increased in the sialylated lactulose groups, while the abundance of Bacteroidetes was decreased.					
31659420	6	68	theme	sialylated	1090:1099	arg1	intervention					1111:1122	sialylated lactulose intervention	1090:1122	sialylated lactulose intervention	1090:1122	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	7	69	theme	Neu5Ac-α2,3-lactulose	1573:1593	arg1	group					1595:1599	the Neu5Ac-α2,3-lactulose group	1569:1599	the Neu5Ac-α2,3-lactulose group	1569:1599	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	4	70	theme	V3-V4	874:878	arg1	region					880:885	the V3-V4 region	870:885	the V3-V4 region of the 16S rDNA gene	870:906	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	6	71	theme	S24-7	1174:1178	arg1	group					1180:1184	Bacteroidales S24-7 group	1160:1184	Bacteroidales S24-7 group	1160:1184	At the family level, sialylated lactulose intervention decreased the relative abundance of Bacteroidales S24-7 group and Helicobacteraceae and enhanced the abundance of Lactobacillaceae, which reflects the modulatory effect of sialylated lactulose on intestinal microbiota.					
31659420	8	72	theme	control	1788:1794	arg1	group					1796:1800	the normal control group	1777:1800	the normal control group	1777:1800	The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.					
31659420	1	73	theme	beneficial	156:165	arg1	effects					167:173	beneficial effects	156:173	beneficial effects	156:173	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31659420	7	74	theme	Diversity	1343:1351	arg1	analysis					1353:1360	Diversity analysis	1343:1360	Diversity analysis	1343:1360	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	0	75	theme	high-throughput	82:96	arg1	sequencing					98:107	Illumina high-throughput sequencing	73:107	Illumina high-throughput sequencing	73:107	Effects of sialylated lactulose on the mouse intestinal microbiome using Illumina high-throughput sequencing.					
31659420	9	76	used	used	1839:1842	arg2	component					1865:1873	a functional food component	1847:1873	a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health	1847:2000	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	9	76	used	used	1839:1842	arg2	lactulose					1820:1828	sialylated lactulose	1809:1828	sialylated lactulose	1809:1828	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	7	77	theme	Kdnα-2,6-lactulose	1540:1557	arg1	group					1559:1563	the Kdnα-2,6-lactulose group	1536:1563	the Kdnα-2,6-lactulose group	1536:1563	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	5	78	theme	phylum	916:921	arg1	level					923:927	the phylum level	912:927	the phylum level	912:927	At the phylum level, the relative abundance of Firmicutes was increased in the sialylated lactulose groups, while the abundance of Bacteroidetes was decreased.					
31659420	4	79	theme	gene	903:906	arg1	region					880:885	the V3-V4 region	870:885	the V3-V4 region of the 16S rDNA gene	870:906	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	4	80	theme	mice	734:737	arg1	feces					719:723	the feces	715:723	the feces of three mice from each group	715:753	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	2	81	theme	modulatory	380:389	arg1	effects					391:397	modulatory effects	380:397	modulatory effects	380:397	However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota.					
31659420	9	82	theme	sialylated	1809:1818	arg1	lactulose					1820:1828	sialylated lactulose	1809:1828	sialylated lactulose	1809:1828	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	9	82	theme	sialylated	1809:1818	arg1	component					1865:1873	a functional food component	1847:1873	a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health	1847:2000	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	4	83	theme	administration	699:712	arg1	days					681:684	14 days	678:684	14 days of lactulose administration	678:712	After 14 days of lactulose administration, the feces of three mice from each group were collected, and the intestinal microbiota were detected by Illumina MiSeq high-throughput sequencing targeting the V3-V4 region of the 16S rDNA gene.					
31659420	0	84	theme	lactulose	22:30	arg1	Effects					0:6	Effects	0:6	Effects of sialylated lactulose on the mouse intestinal microbiome	0:65	Effects of sialylated lactulose on the mouse intestinal microbiome using Illumina high-throughput sequencing.					
31659420	1	85	theme	Sialylated	110:119	arg1	oligosaccharides					121:136	Sialylated oligosaccharides	110:136	Sialylated oligosaccharides	110:136	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31659420	2	86	gly	sialylated	353:362	arg1	lactuloses					364:373	sialylated lactuloses	353:373	sialylated lactuloses	353:373	However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota.					
31659420	2	87	theme	intestinal	406:415	arg1	microbiota					417:426	the intestinal microbiota	402:426	the intestinal microbiota	402:426	However, it is unknown whether sialylated lactuloses have modulatory effects on the intestinal microbiota.					
31659420	0	88	theme	intestinal	45:54	arg1	microbiome					56:65	the mouse intestinal microbiome	35:65	the mouse intestinal microbiome	35:65	Effects of sialylated lactulose on the mouse intestinal microbiome using Illumina high-throughput sequencing.					
31659420	0	89	gly	sialylated	11:20	arg1	lactulose					22:30	sialylated lactulose	11:30	sialylated lactulose	11:30	Effects of sialylated lactulose on the mouse intestinal microbiome using Illumina high-throughput sequencing.					
31659420	7	90	theme	Simpson	1495:1501	arg1	indices					1513:1519	the Shannon and Simpson diversity indices	1479:1519	the Shannon and Simpson diversity indices	1479:1519	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	7	90	theme	Simpson	1495:1501	arg1	higher					1526:1531	higher	1526:1531	higher	1526:1531	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	5	91	theme	relative	934:941	arg1	abundance					943:951	the relative abundance	930:951	the relative abundance of Firmicutes	930:965	At the phylum level, the relative abundance of Firmicutes was increased in the sialylated lactulose groups, while the abundance of Bacteroidetes was decreased.					
31659420	9	92	theme	food	1860:1863	arg1	component					1865:1873	a functional food component	1847:1873	a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health	1847:2000	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	9	92	theme	food	1860:1863	arg1	lactulose					1820:1828	sialylated lactulose	1809:1828	sialylated lactulose	1809:1828	Thus, sialylated lactulose could be used as a functional food component with potential therapeutic applications in manipulating intestinal microbiota to exert beneficial effects on the host's health.					
31659420	8	93	theme	intestinal	1654:1663	arg1	composition					1683:1693	the intestinal microbiota sample composition	1650:1693	the intestinal microbiota sample composition	1650:1693	The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.					
31659420	7	94	theme	Shannon	1483:1489	arg1	indices					1513:1519	the Shannon and Simpson diversity indices	1479:1519	the Shannon and Simpson diversity indices	1479:1519	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	7	94	theme	Shannon	1483:1489	arg1	higher					1526:1531	higher	1526:1531	higher	1526:1531	Diversity analysis indicated that the index of Chao was higher in the sialylated lactulose groups than in the normal control group, and the Shannon and Simpson diversity indices were higher in the Kdnα-2,6-lactulose group and the Neu5Ac-α2,3-lactulose group than in the normal control group.					
31659420	1	95	contain	have	151:154	arg2	effects					167:173	beneficial effects	156:173	beneficial effects	156:173	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31659420	1	95	contain	have	151:154	arg1	oligosaccharides					121:136	Sialylated oligosaccharides	110:136	Sialylated oligosaccharides	110:136	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31659420	8	96	theme	sample	1676:1681	arg1	composition					1683:1693	the intestinal microbiota sample composition	1650:1693	the intestinal microbiota sample composition	1650:1693	The results of the intestinal microbiota sample composition indicated that there were differences between the sialylated lactulose groups and the normal control group.					
31659420	1	97	theme	blood	247:251	arg1	endotoxin					253:261	blood endotoxin	247:261	blood endotoxin	247:261	Sialylated oligosaccharides are known to have beneficial effects, such as increasing the level of bifidobacteria, reducing the levels of blood endotoxin and blood ammonia, and enhancing the body's immune system.					
31362021	7	0	theme	biofilm	1588:1594	arg1	damage					1596:1601	biofilm damage	1588:1601	biofilm damage	1588:1601	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	11	1	theme	A549	2228:2231	arg1	cells					2233:2237	A549 cells	2228:2237	A549 cells	2228:2237	Composite prompted 50% of cytotoxicity at 80 μg/mL unmistakably uncovers cytotoxicity against A549 cells.					
31362021	13	2	theme	apoptosis-like	2586:2599	arg1	death					2615:2619	apoptosis-like necrotic cell death	2586:2619	apoptosis-like necrotic cell death	2586:2619	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	14	3	theme	present	2626:2632	arg1	findings					2634:2641	The present findings	2622:2641	The present findings	2622:2641	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	1	4	theme	iron	279:282	arg1	nanoparticles					290:302	fundamentally adjusted iron oxide nanoparticles	256:302	fundamentally adjusted iron oxide nanoparticles	256:302	The logical research on fundamentally adjusted iron oxide nanoparticles has turned out to expanded in biomedicine because of the improved activity and best biocompatibility.					
31362021	4	5	theme	nanocomposite	926:938	arg1	morphology					896:905	Particle morphology	887:905	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM)	887:983	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	6	6	theme	composite	1249:1257	arg1	phase					1211:1215	Crystalline phase	1199:1215	Crystalline phase	1199:1215	Crystalline phase and magnetisation impact of the composite resolved from XRD and VSM studies.					
31362021	6	6	theme	composite	1249:1257	arg1	impact					1235:1240	magnetisation impact	1221:1240	magnetisation impact	1221:1240	Crystalline phase and magnetisation impact of the composite resolved from XRD and VSM studies.					
31362021	3	7	theme	test	760:763	arg1	VSM					779:781	VSM	779:781	VSM	779:781	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	7	theme	test	760:763	arg1	magnetometer					765:776	Vibrating test magnetometer	750:776	Vibrating test magnetometer (VSM)	750:782	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	9	8	theme	Biofilm	1765:1771	arg1	restraint					1773:1781	Biofilm restraint	1765:1781	Biofilm restraint	1765:1781	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	12	9	theme	Nanocomposite	2240:2252	arg1	treatment					2254:2262	Nanocomposite treatment	2240:2262	Nanocomposite treatment	2240:2262	Nanocomposite treatment revealed a high decrease of cell feasibility at all the fixation and most extreme impact seen in 100 μg.					
31362021	0	10	theme	malignant	214:222	arg1	growth					224:229	malignant growth	214:229	malignant growth	214:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	10	theme	malignant	214:222	arg1	activities					132:141	the upgraded bio-restorative and pharmacotherapeutic activities	79:141	the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth	79:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	3	11	dep	microscopy	656:665	arg1	studies					784:790	studies	784:790	studies	784:790	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	7	12	theme	inhibition	1553:1562	arg1	concentration					1564:1576	least minimum inhibition concentration	1539:1576	least minimum inhibition concentration (MIC)	1539:1582	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	7	12	theme	inhibition	1553:1562	arg1	inhibition					1527:1536	inhibition	1527:1536	inhibition	1527:1536	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	7	12	theme	inhibition	1553:1562	arg1	MIC					1579:1581	MIC	1579:1581	MIC	1579:1581	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	6	13	theme	magnetisation	1221:1233	arg1	impact					1235:1240	magnetisation impact	1221:1240	magnetisation impact	1221:1240	Crystalline phase and magnetisation impact of the composite resolved from XRD and VSM studies.					
31362021	3	14	theme	scanning	638:645	arg1	XRD					741:743	XRD	741:743	XRD	741:743	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	14	theme	scanning	638:645	arg1	SEM					668:670	SEM	668:670	SEM	668:670	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	14	theme	scanning	638:645	arg1	Fourier					674:680	Fourier	674:680	Fourier transform infrared spectroscopy (FTIR)	674:719	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	14	theme	scanning	638:645	arg1	microscopy					656:665	scanning electron microscopy	638:665	scanning electron microscopy (SEM)	638:671	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	14	theme	scanning	638:645	arg1	diffraction					728:738	x-ray diffraction	722:738	x-ray diffraction	722:738	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	13	15	theme	atomic	2463:2468	arg1	material					2470:2477	atomic material	2463:2477	atomic material related to trademark changes in against oxidative enzymes	2463:2535	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	7	16	theme	tried	1474:1478	arg1	strains					1490:1496	the tried bacterial strains	1470:1496	the tried bacterial strains	1470:1496	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	0	17	theme	bio-restorative	92:106	arg1	growth					224:229	malignant growth	214:229	malignant growth	214:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	17	theme	bio-restorative	92:106	arg1	activities					132:141	the upgraded bio-restorative and pharmacotherapeutic activities	79:141	the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth	79:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	17	theme	bio-restorative	92:106	arg1	nanoformulation					161:175	Supra molecular nanoformulation	145:175	Supra molecular nanoformulation against drug-resistant pathogens	145:208	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	3	18	dep	spherical	819:827	arg1	electron-dense					830:843	electron-dense	830:843	electron-dense	830:843	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	19	theme	nanocomposite	621:633	arg1	Characterisation					585:600	Characterisation	585:600	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies	585:790	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	12	20	from	fixation	2320:2327	arg1	decrease					2280:2287	a high decrease	2273:2287	a high decrease of cell feasibility at all the fixation	2273:2327	Nanocomposite treatment revealed a high decrease of cell feasibility at all the fixation and most extreme impact seen in 100 μg.					
31362021	12	20	from	fixation	2320:2327	arg1	impact					2346:2351	most extreme impact	2333:2351	most extreme impact seen in 100 μg	2333:2366	Nanocomposite treatment revealed a high decrease of cell feasibility at all the fixation and most extreme impact seen in 100 μg.					
31362021	13	21	theme	cell	2430:2433	arg1	morphology					2435:2444	cell morphology	2430:2444	cell morphology	2430:2444	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	13	21	theme	cell	2430:2433	arg1	build-up					2451:2458	the build-up	2447:2458	the build-up of atomic material related to trademark changes in against oxidative enzymes	2447:2535	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	13	21	theme	cell	2430:2433	arg1	design					2559:2564	quality articulation design	2538:2564	quality articulation design	2538:2564	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	7	22	theme	bacterial	1480:1488	arg1	strains					1490:1496	the tried bacterial strains	1470:1496	the tried bacterial strains	1470:1496	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	10	23	theme	Bax	2099:2101	arg1	assurance					2004:2012	the assurance	2000:2012	the assurance of cell feasibility as opposed to oxidative proteins	2000:2065	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	10	23	theme	Bax	2099:2101	arg1	example					2081:2087	articulation example	2068:2087	articulation example of TNF-α, Bax, PARP qualities and apoptosis	2068:2131	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	0	24	theme	pharmacotherapeutic	112:130	arg1	growth					224:229	malignant growth	214:229	malignant growth	214:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	24	theme	pharmacotherapeutic	112:130	arg1	activities					132:141	the upgraded bio-restorative and pharmacotherapeutic activities	79:141	the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth	79:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	24	theme	pharmacotherapeutic	112:130	arg1	nanoformulation					161:175	Supra molecular nanoformulation	145:175	Supra molecular nanoformulation against drug-resistant pathogens	145:208	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	9	25	theme	solubilised	1893:1903	arg1	biofilm					1905:1911	ethanol solubilised biofilm	1885:1911	ethanol solubilised biofilm of both tested strains	1885:1934	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	14	26	theme	malignant	2775:2783	arg1	cells					2792:2796	malignant growth cells	2775:2796	malignant growth cells	2775:2796	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	4	27	theme	scanning	950:957	arg1	SEM					980:982	SEM	980:982	SEM	980:982	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	4	27	theme	scanning	950:957	arg1	microscopy					968:977	scanning electron microscopy	950:977	scanning electron microscopy (SEM)	950:983	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	10	28	theme	TNF-α	2092:2096	arg1	assurance					2004:2012	the assurance	2000:2012	the assurance of cell feasibility as opposed to oxidative proteins	2000:2065	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	10	28	theme	TNF-α	2092:2096	arg1	example					2081:2087	articulation example	2068:2087	articulation example of TNF-α, Bax, PARP qualities and apoptosis	2068:2131	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	13	29	theme	necrotic	2601:2608	arg1	death					2615:2619	apoptosis-like necrotic cell death	2586:2619	apoptosis-like necrotic cell death	2586:2619	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	0	30	theme	molecular	151:159	arg1	activities					132:141	the upgraded bio-restorative and pharmacotherapeutic activities	79:141	the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth	79:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	30	theme	molecular	151:159	arg1	nanoformulation					161:175	Supra molecular nanoformulation	145:175	Supra molecular nanoformulation against drug-resistant pathogens	145:208	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	3	31	theme	50-60 nm	877:884	arg1	particles					864:872	rough particles	858:872	rough particles of 50-60 nm	858:884	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	14	32	theme	safe	2745:2748	arg1	pathogens					2761:2769	safe medication pathogens	2745:2769	safe medication pathogens	2745:2769	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	2	33	theme	eco-friendly	543:554	arg1	technique					574:582	eco-friendly in situ substance technique	543:582	eco-friendly in situ substance technique	543:582	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
31362021	3	34	theme	spherical	819:827	arg1	core					845:848	highly stable spherical, electron-dense core	805:848	highly stable spherical, electron-dense core	805:848	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	7	35	theme	Anti-bacterial	1294:1307	arg1	activity					1309:1316	Anti-bacterial activity	1294:1316	Anti-bacterial activity of the nanocomposite	1294:1337	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	5	36	theme	iron	1173:1176	arg1	oxide					1178:1182	iron oxide	1173:1182	iron oxide	1173:1182	FTIR studies show that the specific interaction of practical gatherings of chitosan with iron oxide nanoparticles.					
31362021	14	37	theme	medication	2750:2759	arg1	pathogens					2761:2769	safe medication pathogens	2745:2769	safe medication pathogens	2745:2769	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	9	38	theme	optical	1866:1872	arg1	density					1874:1880	lower optical density	1860:1880	lower optical density of ethanol solubilised biofilm of both tested strains	1860:1934	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	1	39	from	research	244:251	arg1	nanoparticles					290:302	fundamentally adjusted iron oxide nanoparticles	256:302	fundamentally adjusted iron oxide nanoparticles	256:302	The logical research on fundamentally adjusted iron oxide nanoparticles has turned out to expanded in biomedicine because of the improved activity and best biocompatibility.					
31362021	10	40	theme	oxidative	2048:2056	arg1	proteins					2058:2065	oxidative proteins	2048:2065	oxidative proteins	2048:2065	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	1	41	theme	improved	361:368	arg1	activity					370:377	the improved activity	357:377	the improved activity	357:377	The logical research on fundamentally adjusted iron oxide nanoparticles has turned out to expanded in biomedicine because of the improved activity and best biocompatibility.					
31362021	13	42	theme	treated	2383:2389	arg1	cells					2391:2395	Nanocomposite treated cells	2369:2395	Nanocomposite treated cells	2369:2395	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	9	43	from	high	1815:1818	arg1	treatment					1837:1845	nanocomposite treatment	1823:1845	nanocomposite treatment	1823:1845	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	5	44	theme	gatherings	1145:1154	arg1	interaction					1120:1130	the specific interaction	1107:1130	the specific interaction of practical gatherings of chitosan with iron oxide	1107:1182	FTIR studies show that the specific interaction of practical gatherings of chitosan with iron oxide nanoparticles.					
31362021	14	45	theme	nanocomposite	2702:2714	arg1	remedial					2728:2735	a viable remedial	2719:2735	a viable remedial against safe medication pathogens and malignant growth cells	2719:2796	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	14	45	theme	nanocomposite	2702:2714	arg1	usage					2673:2677	the conceivable usage	2657:2677	the conceivable usage of chitosan‑iron oxide nanocomposite	2657:2714	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	4	46	theme	electron	1011:1018	arg1	centre					1020:1025	thick electron centre	1005:1025	thick electron centre	1005:1025	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	5	47	theme	chitosan	1159:1166	arg1	gatherings					1145:1154	practical gatherings	1135:1154	practical gatherings of chitosan	1135:1166	FTIR studies show that the specific interaction of practical gatherings of chitosan with iron oxide nanoparticles.					
31362021	4	48	theme	50-60 nm	1074:1081	arg1	scope					1065:1069	the size scope	1056:1069	the size scope of 50-60 nm	1056:1081	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	7	49	theme	readied	1405:1411	arg1	nanocomposite					1413:1425	the readied nanocomposite	1401:1425	the readied nanocomposite	1401:1425	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	9	50	theme	tested	1921:1926	arg1	strains					1928:1934	both tested strains	1916:1934	both tested strains	1916:1934	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	2	51	theme	pharmacotherapeutic	456:474	arg1	property					476:483	bio-restorative and pharmacotherapeutic property	436:483	bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique	436:582	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
31362021	2	52	dep	in	556:557	arg1	situ					559:562	situ	559:562	situ	559:562	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
31362021	14	53	theme	oxide	2696:2700	arg1	nanocomposite					2702:2714	chitosan‑iron oxide nanocomposite	2682:2714	chitosan‑iron oxide nanocomposite	2682:2714	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	13	54	theme	articulation	2546:2557	arg1	morphology					2435:2444	cell morphology	2430:2444	cell morphology	2430:2444	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	13	54	theme	articulation	2546:2557	arg1	design					2559:2564	quality articulation design	2538:2564	quality articulation design	2538:2564	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	8	55	theme	nanocomposites	1654:1667	arg1	dosages					1643:1649	100 μg dosages	1636:1649	100 μg dosages of nanocomposites	1636:1667	100 μg dosages of nanocomposites recorded 20.0 and 21.0 mm of the zone of inhibition against E. coli and S. aureus respectively.					
31362021	12	56	theme	feasibility	2297:2307	arg1	decrease					2280:2287	a high decrease	2273:2287	a high decrease of cell feasibility at all the fixation	2273:2327	Nanocomposite treatment revealed a high decrease of cell feasibility at all the fixation and most extreme impact seen in 100 μg.					
31362021	12	56	theme	feasibility	2297:2307	arg1	impact					2346:2351	most extreme impact	2333:2351	most extreme impact seen in 100 μg	2333:2366	Nanocomposite treatment revealed a high decrease of cell feasibility at all the fixation and most extreme impact seen in 100 μg.					
31362021	5	57	with	interaction	1120:1130	arg1	oxide					1178:1182	iron oxide	1173:1182	iron oxide	1173:1182	FTIR studies show that the specific interaction of practical gatherings of chitosan with iron oxide nanoparticles.					
31362021	2	58	theme	bio-restorative	436:450	arg1	property					476:483	bio-restorative and pharmacotherapeutic property	436:483	bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique	436:582	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
31362021	7	59	theme	tested	1620:1625	arg1	strains					1627:1633	both tested strains	1615:1633	the both tested strains	1611:1633	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	10	60	theme	PARP	2104:2107	arg1	qualities					2109:2117	PARP qualities	2104:2117	PARP qualities	2104:2117	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	10	61	dep	assurance	2004:2012	arg1	opposed					2037:2043	opposed	2037:2043	opposed to oxidative proteins	2037:2065	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	0	62	theme	Chitosan	42:49	arg1	nanocomposite					51:63	Chitosan nanocomposite	42:63	Chitosan nanocomposite	42:63	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	14	63	theme	conceivable	2661:2671	arg1	remedial					2728:2735	a viable remedial	2719:2735	a viable remedial against safe medication pathogens and malignant growth cells	2719:2796	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	14	63	theme	conceivable	2661:2671	arg1	usage					2673:2677	the conceivable usage	2657:2677	the conceivable usage of chitosan‑iron oxide nanocomposite	2657:2714	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	5	64	theme	FTIR	1084:1087	arg1	studies					1089:1095	FTIR studies	1084:1095	FTIR studies	1084:1095	FTIR studies show that the specific interaction of practical gatherings of chitosan with iron oxide nanoparticles.					
31362021	7	65	theme	bacterial	1362:1370	arg1	pathogens					1372:1380	human bacterial pathogens	1356:1380	human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains	1356:1633	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	12	66	theme	high	2275:2278	arg1	decrease					2280:2287	a high decrease	2273:2287	a high decrease of cell feasibility at all the fixation	2273:2327	Nanocomposite treatment revealed a high decrease of cell feasibility at all the fixation and most extreme impact seen in 100 μg.					
31362021	1	67	theme	logical	236:242	arg1	research					244:251	The logical research	232:251	The logical research on fundamentally adjusted iron oxide nanoparticles	232:302	The logical research on fundamentally adjusted iron oxide nanoparticles has turned out to expanded in biomedicine because of the improved activity and best biocompatibility.					
31362021	10	68	theme	apoptosis	2123:2131	arg1	assurance					2004:2012	the assurance	2000:2012	the assurance of cell feasibility as opposed to oxidative proteins	2000:2065	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	10	68	theme	apoptosis	2123:2131	arg1	example					2081:2087	articulation example	2068:2087	articulation example of TNF-α, Bax, PARP qualities and apoptosis	2068:2131	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	9	69	theme	biofilm	1905:1911	arg1	density					1874:1880	lower optical density	1860:1880	lower optical density of ethanol solubilised biofilm of both tested strains	1860:1934	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	10	70	theme	cell	1987:1990	arg1	line					1992:1995	the A549 cell line	1978:1995	the A549 cell line	1978:1995	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	13	71	theme	trademark	2490:2498	arg1	changes					2500:2506	trademark changes	2490:2506	trademark changes in against oxidative enzymes	2490:2535	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	7	72	theme	nanocomposite	1325:1337	arg1	activity					1309:1316	Anti-bacterial activity	1294:1316	Anti-bacterial activity of the nanocomposite	1294:1337	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	10	73	theme	cell	2017:2020	arg1	feasibility					2022:2032	cell feasibility	2017:2032	cell feasibility	2017:2032	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	0	74	theme	drug-resistant	185:198	arg1	pathogens					200:208	drug-resistant pathogens	185:208	drug-resistant pathogens	185:208	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	1	75	theme	adjusted	270:277	arg1	nanoparticles					290:302	fundamentally adjusted iron oxide nanoparticles	256:302	fundamentally adjusted iron oxide nanoparticles	256:302	The logical research on fundamentally adjusted iron oxide nanoparticles has turned out to expanded in biomedicine because of the improved activity and best biocompatibility.					
31362021	1	76	theme	oxide	284:288	arg1	nanoparticles					290:302	fundamentally adjusted iron oxide nanoparticles	256:302	fundamentally adjusted iron oxide nanoparticles	256:302	The logical research on fundamentally adjusted iron oxide nanoparticles has turned out to expanded in biomedicine because of the improved activity and best biocompatibility.					
31362021	7	77	theme	maximum	1511:1517	arg1	zone					1519:1522	maximum zone	1511:1522	maximum zone of inhibition, least minimum inhibition concentration (MIC)	1511:1582	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	4	78	theme	synthesised	914:924	arg1	nanocomposite					926:938	the synthesised nanocomposite	910:938	the synthesised nanocomposite utilising scanning electron microscopy (SEM)	910:983	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	7	79	theme	least	1539:1543	arg1	concentration					1564:1576	least minimum inhibition concentration	1539:1576	least minimum inhibition concentration (MIC)	1539:1582	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	7	79	theme	least	1539:1543	arg1	inhibition					1527:1536	inhibition	1527:1536	inhibition	1527:1536	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	7	79	theme	least	1539:1543	arg1	MIC					1579:1581	MIC	1579:1581	MIC	1579:1581	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	2	80	theme	nanocomposite	508:520	arg1	property					476:483	bio-restorative and pharmacotherapeutic property	436:483	bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique	436:582	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
31362021	7	81	theme	minimum	1545:1551	arg1	concentration					1564:1576	least minimum inhibition concentration	1539:1576	least minimum inhibition concentration (MIC)	1539:1582	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	7	81	theme	minimum	1545:1551	arg1	inhibition					1527:1536	inhibition	1527:1536	inhibition	1527:1536	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	7	81	theme	minimum	1545:1551	arg1	MIC					1579:1581	MIC	1579:1581	MIC	1579:1581	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	7	82	theme	inhibition	1527:1536	arg1	damage					1596:1601	biofilm damage	1588:1601	biofilm damage	1588:1601	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	7	82	theme	inhibition	1527:1536	arg1	zone					1519:1522	maximum zone	1511:1522	maximum zone of inhibition, least minimum inhibition concentration (MIC)	1511:1582	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	3	83	theme	Vibrating	750:758	arg1	VSM					779:781	VSM	779:781	VSM	779:781	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	83	theme	Vibrating	750:758	arg1	magnetometer					765:776	Vibrating test magnetometer	750:776	Vibrating test magnetometer (VSM)	750:782	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	13	84	from	changes	2419:2425	arg1	morphology					2435:2444	cell morphology	2430:2444	cell morphology	2430:2444	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	13	84	from	changes	2419:2425	arg1	build-up					2451:2458	the build-up	2447:2458	the build-up of atomic material related to trademark changes in against oxidative enzymes	2447:2535	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	13	84	from	changes	2419:2425	arg1	design					2559:2564	quality articulation design	2538:2564	quality articulation design	2538:2564	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	4	85	theme	Particle	887:894	arg1	morphology					896:905	Particle morphology	887:905	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM)	887:983	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	10	86	theme	articulation	2068:2079	arg1	assurance					2004:2012	the assurance	2000:2012	the assurance of cell feasibility as opposed to oxidative proteins	2000:2065	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	10	86	theme	articulation	2068:2079	arg1	example					2081:2087	articulation example	2068:2087	articulation example of TNF-α, Bax, PARP qualities and apoptosis	2068:2131	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	13	87	theme	oxidative	2519:2527	arg1	enzymes					2529:2535	oxidative enzymes	2519:2535	oxidative enzymes	2519:2535	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	3	88	theme	synthesised	609:619	arg1	nanocomposite					621:633	the synthesised nanocomposite	605:633	the synthesised nanocomposite	605:633	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	11	89	theme	cytotoxicity	2160:2171	arg1	cytotoxicity					2160:2171	cytotoxicity	2160:2171	cytotoxicity	2160:2171	Composite prompted 50% of cytotoxicity at 80 μg/mL unmistakably uncovers cytotoxicity against A549 cells.					
31362021	11	89	theme	cytotoxicity	2160:2171	arg1	%					2155:2155	50%	2153:2155	50% of cytotoxicity	2153:2171	Composite prompted 50% of cytotoxicity at 80 μg/mL unmistakably uncovers cytotoxicity against A549 cells.					
31362021	6	90	theme	Crystalline	1199:1209	arg1	phase					1211:1215	Crystalline phase	1199:1215	Crystalline phase	1199:1215	Crystalline phase and magnetisation impact of the composite resolved from XRD and VSM studies.					
31362021	3	91	dep	Fourier	674:680	arg1	transform					682:690	transform	682:690	transform infrared spectroscopy (FTIR)	682:719	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	14	92	theme	growth	2785:2790	arg1	cells					2792:2796	malignant growth cells	2775:2796	malignant growth cells	2775:2796	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	7	93	theme	strains	1490:1496	arg1	development					1455:1465	the development	1451:1465	the development of the tried bacterial strains	1451:1496	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	3	94	theme	stable	812:817	arg1	core					845:848	highly stable spherical, electron-dense core	805:848	highly stable spherical, electron-dense core	805:848	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	0	95	theme	Supra	145:149	arg1	activities					132:141	the upgraded bio-restorative and pharmacotherapeutic activities	79:141	the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth	79:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	95	theme	Supra	145:149	arg1	nanoformulation					161:175	Supra molecular nanoformulation	145:175	Supra molecular nanoformulation against drug-resistant pathogens	145:208	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	5	96	theme	practical	1135:1143	arg1	gatherings					1145:1154	practical gatherings	1135:1154	practical gatherings of chitosan	1135:1166	FTIR studies show that the specific interaction of practical gatherings of chitosan with iron oxide nanoparticles.					
31362021	3	97	theme	electron	647:654	arg1	XRD					741:743	XRD	741:743	XRD	741:743	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	97	theme	electron	647:654	arg1	SEM					668:670	SEM	668:670	SEM	668:670	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	97	theme	electron	647:654	arg1	Fourier					674:680	Fourier	674:680	Fourier transform infrared spectroscopy (FTIR)	674:719	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	97	theme	electron	647:654	arg1	microscopy					656:665	scanning electron microscopy	638:665	scanning electron microscopy (SEM)	638:671	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	97	theme	electron	647:654	arg1	diffraction					728:738	x-ray diffraction	722:738	x-ray diffraction	722:738	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	9	98	theme	nanocomposite	1823:1835	arg1	treatment					1837:1845	nanocomposite treatment	1823:1845	nanocomposite treatment	1823:1845	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	6	99	theme	VSM	1281:1283	arg1	studies					1285:1291	VSM studies	1281:1291	VSM studies	1281:1291	Crystalline phase and magnetisation impact of the composite resolved from XRD and VSM studies.					
31362021	13	100	theme	cell	2610:2613	arg1	death					2615:2619	apoptosis-like necrotic cell death	2586:2619	apoptosis-like necrotic cell death	2586:2619	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	13	101	theme	striking	2410:2417	arg1	changes					2419:2425	striking changes	2410:2425	striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death	2410:2619	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	9	102	from	treatment	1837:1845	arg1	high					1815:1818	high	1815:1818	high	1815:1818	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	4	103	theme	electron	959:966	arg1	SEM					980:982	SEM	980:982	SEM	980:982	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	4	103	theme	electron	959:966	arg1	microscopy					968:977	scanning electron microscopy	950:977	scanning electron microscopy (SEM)	950:983	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	10	104	theme	qualities	2109:2117	arg1	assurance					2004:2012	the assurance	2000:2012	the assurance of cell feasibility as opposed to oxidative proteins	2000:2065	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	10	104	theme	qualities	2109:2117	arg1	example					2081:2087	articulation example	2068:2087	articulation example of TNF-α, Bax, PARP qualities and apoptosis	2068:2131	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	10	105	theme	Anticancer	1937:1946	arg1	activity					1948:1955	Anticancer activity	1937:1955	Anticancer activity	1937:1955	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	0	106	dep	activities	132:141	arg1	growth					224:229	malignant growth	214:229	malignant growth	214:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	106	dep	activities	132:141	arg1	activities					132:141	the upgraded bio-restorative and pharmacotherapeutic activities	79:141	the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth	79:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	106	dep	activities	132:141	arg1	nanoformulation					161:175	Supra molecular nanoformulation	145:175	Supra molecular nanoformulation against drug-resistant pathogens	145:208	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	9	107	theme	lower	1860:1864	arg1	density					1874:1880	lower optical density	1860:1880	lower optical density of ethanol solubilised biofilm of both tested strains	1860:1934	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	9	108	theme	ethanol	1885:1891	arg1	biofilm					1905:1911	ethanol solubilised biofilm	1885:1911	ethanol solubilised biofilm of both tested strains	1885:1934	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	13	109	theme	Nanocomposite	2369:2381	arg1	cells					2391:2395	Nanocomposite treated cells	2369:2395	Nanocomposite treated cells	2369:2395	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	14	110	theme	viable	2721:2726	arg1	usage					2673:2677	the conceivable usage	2657:2677	the conceivable usage of chitosan‑iron oxide nanocomposite	2657:2714	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	14	110	theme	viable	2721:2726	arg1	remedial					2728:2735	a viable remedial	2719:2735	a viable remedial against safe medication pathogens and malignant growth cells	2719:2796	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	4	111	theme	harsh	1035:1039	arg1	particles					1041:1049	harsh particles	1035:1049	harsh particles	1035:1049	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	5	112	theme	specific	1111:1118	arg1	interaction					1120:1130	the specific interaction	1107:1130	the specific interaction of practical gatherings of chitosan with iron oxide	1107:1182	FTIR studies show that the specific interaction of practical gatherings of chitosan with iron oxide nanoparticles.					
31362021	13	113	theme	related	2479:2485	arg1	material					2470:2477	atomic material	2463:2477	atomic material related to trademark changes in against oxidative enzymes	2463:2535	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	2	114	theme	substance	564:572	arg1	technique					574:582	eco-friendly in situ substance technique	543:582	eco-friendly in situ substance technique	543:582	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
31362021	12	115	theme	extreme	2338:2344	arg1	impact					2346:2351	most extreme impact	2333:2351	most extreme impact seen in 100 μg	2333:2366	Nanocomposite treatment revealed a high decrease of cell feasibility at all the fixation and most extreme impact seen in 100 μg.					
31362021	3	116	theme	rough	858:862	arg1	particles					864:872	rough particles	858:872	rough particles of 50-60 nm	858:884	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	0	117	theme	upgraded	83:90	arg1	growth					224:229	malignant growth	214:229	malignant growth	214:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	117	theme	upgraded	83:90	arg1	activities					132:141	the upgraded bio-restorative and pharmacotherapeutic activities	79:141	the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth	79:229	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	117	theme	upgraded	83:90	arg1	nanoformulation					161:175	Supra molecular nanoformulation	145:175	Supra molecular nanoformulation against drug-resistant pathogens	145:208	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	1	118	theme	best	383:386	arg1	biocompatibility					388:403	best biocompatibility	383:403	best biocompatibility	383:403	The logical research on fundamentally adjusted iron oxide nanoparticles has turned out to expanded in biomedicine because of the improved activity and best biocompatibility.					
31362021	2	119	theme	in	556:557	arg1	technique					574:582	eco-friendly in situ substance technique	543:582	eco-friendly in situ substance technique	543:582	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
31362021	8	120	theme	100 μg	1636:1641	arg1	dosages					1643:1649	100 μg dosages	1636:1649	100 μg dosages of nanocomposites	1636:1667	100 μg dosages of nanocomposites recorded 20.0 and 21.0 mm of the zone of inhibition against E. coli and S. aureus respectively.					
31362021	2	121	theme	present	414:420	arg1	work					422:425	this present work	409:425	this present work	409:425	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
31362021	0	122	theme	green	2:6	arg1	chemistry					8:16	A green chemistry	0:16	A green chemistry	0:16	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	2	123	theme	chitosan‑iron	488:500	arg1	nanocomposite					508:520	chitosan‑iron oxide nanocomposite	488:520	chitosan‑iron oxide nanocomposite	488:520	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
31362021	13	124	theme	quality	2538:2544	arg1	morphology					2435:2444	cell morphology	2430:2444	cell morphology	2430:2444	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	13	124	theme	quality	2538:2544	arg1	design					2559:2564	quality articulation design	2538:2564	quality articulation design	2538:2564	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	9	125	theme	strains	1928:1934	arg1	biofilm					1905:1911	ethanol solubilised biofilm	1885:1911	ethanol solubilised biofilm of both tested strains	1885:1934	Biofilm restraint was additionally observed to be high in nanocomposite treatment by recording lower optical density of ethanol solubilised biofilm of both tested strains.					
31362021	10	126	theme	A549	1982:1985	arg1	line					1992:1995	the A549 cell line	1978:1995	the A549 cell line	1978:1995	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	0	127	theme	iron	29:32	arg1	CS-IONC					66:72	CS-IONC	66:72	CS-IONC	66:72	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	0	127	theme	iron	29:32	arg1	oxide					34:38	iron oxide	29:38	iron oxide	29:38	A green chemistry to produce iron oxide - Chitosan nanocomposite (CS-IONC) for the upgraded bio-restorative and pharmacotherapeutic activities - Supra molecular nanoformulation against drug-resistant pathogens and malignant growth.					
31362021	14	128	theme	chitosan‑iron	2682:2694	arg1	nanocomposite					2702:2714	chitosan‑iron oxide nanocomposite	2682:2714	chitosan‑iron oxide nanocomposite	2682:2714	The present findings would propose the conceivable usage of chitosan‑iron oxide nanocomposite as a viable remedial against safe medication pathogens and malignant growth cells.					
31362021	3	129	theme	infrared	692:699	arg1	FTIR					715:718	FTIR	715:718	FTIR	715:718	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	129	theme	infrared	692:699	arg1	spectroscopy					701:712	infrared spectroscopy	692:712	infrared spectroscopy (FTIR)	692:719	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	12	130	theme	cell	2292:2295	arg1	feasibility					2297:2307	cell feasibility	2292:2307	cell feasibility	2292:2307	Nanocomposite treatment revealed a high decrease of cell feasibility at all the fixation and most extreme impact seen in 100 μg.					
31362021	4	131	theme	thick	1005:1009	arg1	centre					1020:1025	thick electron centre	1005:1025	thick electron centre	1005:1025	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	7	132	theme	human	1356:1360	arg1	pathogens					1372:1380	human bacterial pathogens	1356:1380	human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains	1356:1633	Anti-bacterial activity of the nanocomposite examined against human bacterial pathogens which suggest that the readied nanocomposite successfully restrained the development of the tried bacterial strains by recording maximum zone of inhibition, least minimum inhibition concentration (MIC) and biofilm damage against the both tested strains.					
31362021	4	133	theme	size	1060:1063	arg1	scope					1065:1069	the size scope	1056:1069	the size scope of 50-60 nm	1056:1081	Particle morphology of the synthesised nanocomposite utilising scanning electron microscopy (SEM) uncovers spherical; thick electron centre shelled harsh particles with the size scope of 50-60 nm.					
31362021	3	134	theme	x-ray	722:726	arg1	microscopy					656:665	scanning electron microscopy	638:665	scanning electron microscopy (SEM)	638:671	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	3	134	theme	x-ray	722:726	arg1	diffraction					728:738	x-ray diffraction	722:738	x-ray diffraction	722:738	Characterisation of the synthesised nanocomposite by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction,(XRD) and Vibrating test magnetometer (VSM) studies reveals that highly stable spherical, electron-dense core shelled rough particles of 50-60 nm.					
31362021	13	135	theme	material	2470:2477	arg1	morphology					2435:2444	cell morphology	2430:2444	cell morphology	2430:2444	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	13	135	theme	material	2470:2477	arg1	build-up					2451:2458	the build-up	2447:2458	the build-up of atomic material related to trademark changes in against oxidative enzymes	2447:2535	Nanocomposite treated cells demonstrated striking changes in cell morphology, the build-up of atomic material related to trademark changes in against oxidative enzymes, quality articulation design which brought about apoptosis-like necrotic cell death.					
31362021	8	136	theme	zone	1702:1705	arg1	zone					1702:1705	the zone	1698:1705	the zone of inhibition against E. coli and S. aureus	1698:1749	100 μg dosages of nanocomposites recorded 20.0 and 21.0 mm of the zone of inhibition against E. coli and S. aureus respectively.					
31362021	8	136	theme	zone	1702:1705	arg1	20.0					1678:1681	20.0	1678:1681	20.0	1678:1681	100 μg dosages of nanocomposites recorded 20.0 and 21.0 mm of the zone of inhibition against E. coli and S. aureus respectively.					
31362021	8	137	theme	inhibition	1710:1719	arg1	zone					1702:1705	the zone	1698:1705	the zone of inhibition against E. coli and S. aureus	1698:1749	100 μg dosages of nanocomposites recorded 20.0 and 21.0 mm of the zone of inhibition against E. coli and S. aureus respectively.					
31362021	10	138	theme	feasibility	2022:2032	arg1	assurance					2004:2012	the assurance	2000:2012	the assurance of cell feasibility as opposed to oxidative proteins	2000:2065	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	10	138	theme	feasibility	2022:2032	arg1	example					2081:2087	articulation example	2068:2087	articulation example of TNF-α, Bax, PARP qualities and apoptosis	2068:2131	Anticancer activity was examined against the A549 cell line by the assurance of cell feasibility as opposed to oxidative proteins, articulation example of TNF-α, Bax, PARP qualities and apoptosis.					
31362021	2	139	theme	oxide	502:506	arg1	nanocomposite					508:520	chitosan‑iron oxide nanocomposite	488:520	chitosan‑iron oxide nanocomposite	488:520	In this present work upgraded bio-restorative and pharmacotherapeutic property of chitosan‑iron oxide nanocomposite, which was set up by eco-friendly in situ substance technique.					
30350625	0	0	theme	Human	92:96	arg1	Serum					98:102	Human Serum	92:102	Human Serum	92:102	Chemoenzymatic Approach for the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum.					
30350625	9	1	theme	modified	1313:1320	arg1	sequences					1330:1338	59 O-GalNAc modified peptide sequences	1301:1338	59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum	1301:1392	This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.					
30350625	3	2	from	serum	451:455	arg1	type					418:421	core-1 type	411:421	core-1 type of O-GalNAcylation in human serum	411:455	Herein, we presented a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum.					
30350625	9	3	gly	glycoproteins	1360:1372	arg1	glycoproteins					1360:1372	38 glycoproteins	1357:1372	38 glycoproteins from 50 μL of serum	1357:1392	This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.					
30350625	8	4	theme	O-GalNAcylated	1167:1180	arg1	peptides					1182:1189	O-GalNAcylated peptides	1167:1189	O-GalNAcylated peptides	1167:1189	We have demonstrated that this method was fitted to analyze O-GalNAcylated peptides with high confidence.					
30350625	3	5	from	type	418:421	arg1	serum					451:455	human serum	445:455	human serum	445:455	Herein, we presented a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum.					
30350625	10	6	theme	O-glycoproteome	1465:1479	arg1	analysis					1453:1460	the analysis	1449:1460	the analysis of O-glycoproteome	1449:1479	This method is expected to have broad applications in the analysis of O-glycoproteome.					
30350625	1	7	theme	various	155:161	arg1	glycoproteins					185:197	various N-linked and O-linked glycoproteins	155:197	various N-linked and O-linked glycoproteins	155:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	2	8	link	O-linked	248:255	arg1	glycoproteins					257:269	the serum O-linked glycoproteins	238:269	the serum O-linked glycoproteins	238:269	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	2	8	link	O-linked	248:255	arg1	well-studied					279:290	well-studied	279:290	well-studied	279:290	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	8	9	theme	high	1196:1199	arg1	confidence					1201:1210	high confidence	1196:1210	high confidence	1196:1210	We have demonstrated that this method was fitted to analyze O-GalNAcylated peptides with high confidence.					
30350625	9	10	theme	sequences	1330:1338	arg1	identification					1283:1296	the identification	1279:1296	the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum	1279:1392	This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.					
30350625	1	11	gly	glycoproteins	185:197	arg1	glycoproteins					185:197	various N-linked and O-linked glycoproteins	155:197	various N-linked and O-linked glycoproteins	155:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	9	12	theme	O-GalNAc	1304:1311	arg1	sequences					1330:1338	59 O-GalNAc modified peptide sequences	1301:1338	59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum	1301:1392	This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.					
30350625	2	13	theme	low	334:336	arg1	abundance					338:346	their low abundance	328:346	their low abundance	328:346	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	1	14	contain	contains	146:153	arg2	glycoproteins					185:197	various N-linked and O-linked glycoproteins	155:197	various N-linked and O-linked glycoproteins	155:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	1	14	contain	contains	146:153	arg1	fluid					135:139	a complex body fluid	120:139	a complex body fluid that contains various N-linked and O-linked glycoproteins	120:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	1	14	contain	contains	146:153	arg1	serum					111:115	Human serum	105:115	Human serum	105:115	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	1	15	link	O-linked	176:183	arg1	glycoproteins					185:197	various N-linked and O-linked glycoproteins	155:197	various N-linked and O-linked glycoproteins	155:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	3	16	theme	O-GalNAcylation	426:440	arg1	type					418:421	core-1 type	411:421	core-1 type of O-GalNAcylation in human serum	411:455	Herein, we presented a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum.					
30350625	1	17	theme	N-linked	163:170	arg1	glycoproteins					185:197	various N-linked and O-linked glycoproteins	155:197	various N-linked and O-linked glycoproteins	155:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	0	18	from	Analysis	43:50	arg1	Serum					98:102	Human Serum	92:102	Human Serum	92:102	Chemoenzymatic Approach for the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum.					
30350625	9	19	from	μL	1382:1383	arg1	glycoproteins					1360:1372	38 glycoproteins	1357:1372	38 glycoproteins from 50 μL of serum	1357:1392	This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.					
30350625	5	20	theme	aldehyde	782:789	arg1	groups					791:796	the aldehyde groups	778:796	the aldehyde groups	778:796	In this way, the internal Gal/GalNAc residues were exposed and were oxidized by the galactose oxidase to carry the aldehyde groups.					
30350625	4	21	theme	tryptic	480:486	arg1	digest					488:493	the tryptic digest	476:493	the tryptic digest of serum	476:502	In this approach, the tryptic digest of serum was first subjected to PNGase F treatment to release the N-glycan and was then treated with strong acid to release sialic acid residues from mucin-type O-glycans.					
30350625	1	22	link	N-linked	163:170	arg1	glycoproteins					185:197	various N-linked and O-linked glycoproteins	155:197	various N-linked and O-linked glycoproteins	155:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	1	23	theme	O-linked	176:183	arg1	glycoproteins					185:197	various N-linked and O-linked glycoproteins	155:197	various N-linked and O-linked glycoproteins	155:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	0	24	theme	Chemoenzymatic	0:13	arg1	Approach					15:22	Chemoenzymatic Approach	0:22	Chemoenzymatic Approach for the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum	0:102	Chemoenzymatic Approach for the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum.					
30350625	9	25	theme	human	1248:1252	arg1	serum					1254:1258	human serum	1248:1258	human serum	1248:1258	This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.					
30350625	5	26	contain	carry	772:776	arg1	residues					704:711	the internal Gal/GalNAc residues	680:711	the internal Gal/GalNAc residues	680:711	In this way, the internal Gal/GalNAc residues were exposed and were oxidized by the galactose oxidase to carry the aldehyde groups.					
30350625	5	26	contain	carry	772:776	arg2	groups					791:796	the aldehyde groups	778:796	the aldehyde groups	778:796	In this way, the internal Gal/GalNAc residues were exposed and were oxidized by the galactose oxidase to carry the aldehyde groups.					
30350625	4	27	theme	mucin-type	645:654	arg1	O-glycans					656:664	mucin-type O-glycans	645:664	mucin-type O-glycans	645:664	In this approach, the tryptic digest of serum was first subjected to PNGase F treatment to release the N-glycan and was then treated with strong acid to release sialic acid residues from mucin-type O-glycans.					
30350625	3	28	theme	novel	372:376	arg1	method					393:398	a novel chemoenzymatic method	370:398	a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum	370:455	Herein, we presented a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum.					
30350625	10	29	theme	broad	1427:1431	arg1	applications					1433:1444	broad applications	1427:1444	broad applications	1427:1444	This method is expected to have broad applications in the analysis of O-glycoproteome.					
30350625	1	30	theme	Human	105:109	arg1	fluid					135:139	a complex body fluid	120:139	a complex body fluid that contains various N-linked and O-linked glycoproteins	120:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	1	30	theme	Human	105:109	arg1	serum					111:115	Human serum	105:115	Human serum	105:115	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	2	31	gly	glycoproteins	257:269	arg1	glycoproteins					257:269	the serum O-linked glycoproteins	238:269	the serum O-linked glycoproteins	238:269	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	2	31	gly	glycoproteins	257:269	arg1	well-studied					279:290	well-studied	279:290	well-studied	279:290	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	2	32	theme	high	305:308	arg1	heterogeneity					310:322	their high heterogeneity	299:322	their high heterogeneity	299:322	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	3	33	theme	chemoenzymatic	378:391	arg1	method					393:398	a novel chemoenzymatic method	370:398	a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum	370:455	Herein, we presented a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum.					
30350625	5	34	theme	galactose	751:759	arg1	oxidase					761:767	the galactose oxidase	747:767	the galactose oxidase	747:767	In this way, the internal Gal/GalNAc residues were exposed and were oxidized by the galactose oxidase to carry the aldehyde groups.					
30350625	7	35	gly	N-glycopeptides	979:993	arg2	N-glycopeptides					979:993	N-glycopeptides	979:993	N-glycopeptides	979:993	The de-N-deglycosylation decreased the abundance of N-glycopeptides, the desialylation simplified the O-glycans and the enzymatic oxidization conferred the enrichment specificity.					
30350625	7	35	gly	N-glycopeptides	979:993	arg2	desialylation					1000:1012	the desialylation	996:1012	the desialylation simplified the O-glycans	996:1037	The de-N-deglycosylation decreased the abundance of N-glycopeptides, the desialylation simplified the O-glycans and the enzymatic oxidization conferred the enrichment specificity.					
30350625	5	36	theme	internal	684:691	arg1	residues					704:711	the internal Gal/GalNAc residues	680:711	the internal Gal/GalNAc residues	680:711	In this way, the internal Gal/GalNAc residues were exposed and were oxidized by the galactose oxidase to carry the aldehyde groups.					
30350625	4	37	theme	acid	626:629	arg1	residues					631:638	sialic acid residues	619:638	sialic acid residues	619:638	In this approach, the tryptic digest of serum was first subjected to PNGase F treatment to release the N-glycan and was then treated with strong acid to release sialic acid residues from mucin-type O-glycans.					
30350625	2	38	theme	serum	242:246	arg1	glycoproteins					257:269	the serum O-linked glycoproteins	238:269	the serum O-linked glycoproteins	238:269	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	2	38	theme	serum	242:246	arg1	well-studied					279:290	well-studied	279:290	well-studied	279:290	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	2	39	link	N-linked	214:221	arg1	glycoproteins					223:235	N-linked glycoproteins	214:235	N-linked glycoproteins	214:235	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	0	40	theme	Proteomics	32:41	arg1	Analysis					43:50	the Proteomics Analysis	28:50	the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum	28:102	Chemoenzymatic Approach for the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum.					
30350625	9	41	theme	serum	1388:1392	arg1	μL					1382:1383	50 μL	1379:1383	50 μL of serum	1379:1392	This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.					
30350625	5	42	theme	Gal/GalNAc	693:702	arg1	residues					704:711	the internal Gal/GalNAc residues	680:711	the internal Gal/GalNAc residues	680:711	In this way, the internal Gal/GalNAc residues were exposed and were oxidized by the galactose oxidase to carry the aldehyde groups.					
30350625	4	43	theme	sialic	619:624	arg1	residues					631:638	sialic acid residues	619:638	sialic acid residues	619:638	In this approach, the tryptic digest of serum was first subjected to PNGase F treatment to release the N-glycan and was then treated with strong acid to release sialic acid residues from mucin-type O-glycans.					
30350625	0	44	theme	Mucin-Type	55:64	arg1	O-Glycosylation					73:87	Mucin-Type Core-1 O-Glycosylation	55:87	Mucin-Type Core-1 O-Glycosylation	55:87	Chemoenzymatic Approach for the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum.					
30350625	0	45	theme	Core-1	66:71	arg1	O-Glycosylation					73:87	Mucin-Type Core-1 O-Glycosylation	55:87	Mucin-Type Core-1 O-Glycosylation	55:87	Chemoenzymatic Approach for the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum.					
30350625	7	46	theme	enrichment	1083:1092	arg1	specificity					1094:1104	the enrichment specificity	1079:1104	the enrichment specificity	1079:1104	The de-N-deglycosylation decreased the abundance of N-glycopeptides, the desialylation simplified the O-glycans and the enzymatic oxidization conferred the enrichment specificity.					
30350625	3	47	theme	core-1	411:416	arg1	type					418:421	core-1 type	411:421	core-1 type of O-GalNAcylation in human serum	411:455	Herein, we presented a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum.					
30350625	4	48	theme	F	534:534	arg1	treatment					536:544	PNGase F treatment	527:544	PNGase F treatment	527:544	In this approach, the tryptic digest of serum was first subjected to PNGase F treatment to release the N-glycan and was then treated with strong acid to release sialic acid residues from mucin-type O-glycans.					
30350625	6	49	theme	LC-MS/MS	908:915	arg1	analysis					917:924	LC-MS/MS analysis	908:924	LC-MS/MS analysis	908:924	The oxidized O-GalNAcylated peptides were then captured by hydrazide beads and eluted with methoxylamine for LC-MS/MS analysis.					
30350625	2	50	theme	N-linked	214:221	arg1	glycoproteins					223:235	N-linked glycoproteins	214:235	N-linked glycoproteins	214:235	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	6	51	theme	hydrazide	858:866	arg1	beads					868:872	hydrazide beads	858:872	hydrazide beads	858:872	The oxidized O-GalNAcylated peptides were then captured by hydrazide beads and eluted with methoxylamine for LC-MS/MS analysis.					
30350625	3	52	theme	human	445:449	arg1	serum					451:455	human serum	445:455	human serum	445:455	Herein, we presented a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum.					
30350625	4	53	theme	PNGase	527:532	arg1	treatment					536:544	PNGase F treatment	527:544	PNGase F treatment	527:544	In this approach, the tryptic digest of serum was first subjected to PNGase F treatment to release the N-glycan and was then treated with strong acid to release sialic acid residues from mucin-type O-glycans.					
30350625	6	54	theme	O-GalNAcylated	812:825	arg1	peptides					827:834	The oxidized O-GalNAcylated peptides	799:834	The oxidized O-GalNAcylated peptides	799:834	The oxidized O-GalNAcylated peptides were then captured by hydrazide beads and eluted with methoxylamine for LC-MS/MS analysis.					
30350625	10	55	contain	have	1422:1425	arg2	applications					1433:1444	broad applications	1427:1444	broad applications	1427:1444	This method is expected to have broad applications in the analysis of O-glycoproteome.					
30350625	10	55	contain	have	1422:1425	arg1	method					1400:1405	This method	1395:1405	This method	1395:1405	This method is expected to have broad applications in the analysis of O-glycoproteome.					
30350625	1	56	theme	complex	122:128	arg1	fluid					135:139	a complex body fluid	120:139	a complex body fluid that contains various N-linked and O-linked glycoproteins	120:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	1	56	theme	complex	122:128	arg1	serum					111:115	Human serum	105:115	Human serum	105:115	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	7	57	theme	enzymatic	1047:1055	arg1	oxidization					1057:1067	the enzymatic oxidization	1043:1067	the enzymatic oxidization	1043:1067	The de-N-deglycosylation decreased the abundance of N-glycopeptides, the desialylation simplified the O-glycans and the enzymatic oxidization conferred the enrichment specificity.					
30350625	6	58	theme	oxidized	803:810	arg1	peptides					827:834	The oxidized O-GalNAcylated peptides	799:834	The oxidized O-GalNAcylated peptides	799:834	The oxidized O-GalNAcylated peptides were then captured by hydrazide beads and eluted with methoxylamine for LC-MS/MS analysis.					
30350625	3	59	from	O-GalNAcylation	426:440	arg1	serum					451:455	human serum	445:455	human serum	445:455	Herein, we presented a novel chemoenzymatic method to analyze core-1 type of O-GalNAcylation in human serum.					
30350625	1	60	theme	body	130:133	arg1	fluid					135:139	a complex body fluid	120:139	a complex body fluid that contains various N-linked and O-linked glycoproteins	120:197	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	1	60	theme	body	130:133	arg1	serum					111:115	Human serum	105:115	Human serum	105:115	Human serum is a complex body fluid that contains various N-linked and O-linked glycoproteins.					
30350625	0	61	theme	O-Glycosylation	73:87	arg1	Analysis					43:50	the Proteomics Analysis	28:50	the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum	28:102	Chemoenzymatic Approach for the Proteomics Analysis of Mucin-Type Core-1 O-Glycosylation in Human Serum.					
30350625	9	62	theme	peptide	1322:1328	arg1	sequences					1330:1338	59 O-GalNAc modified peptide sequences	1301:1338	59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum	1301:1392	This method was applied to analyze human serum, which resulted in the identification of 59 O-GalNAc modified peptide sequences corresponding to 38 glycoproteins from 50 μL of serum.					
30350625	2	63	gly	glycoproteins	223:235	arg1	glycoproteins					223:235	N-linked glycoproteins	214:235	N-linked glycoproteins	214:235	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	4	64	theme	serum	498:502	arg1	digest					488:493	the tryptic digest	476:493	the tryptic digest of serum	476:502	In this approach, the tryptic digest of serum was first subjected to PNGase F treatment to release the N-glycan and was then treated with strong acid to release sialic acid residues from mucin-type O-glycans.					
30350625	2	65	theme	O-linked	248:255	arg1	glycoproteins					257:269	the serum O-linked glycoproteins	238:269	the serum O-linked glycoproteins	238:269	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	2	65	theme	O-linked	248:255	arg1	well-studied					279:290	well-studied	279:290	well-studied	279:290	Compared with N-linked glycoproteins, the serum O-linked glycoproteins are not well-studied due to their high heterogeneity and their low abundance.					
30350625	7	66	theme	N-glycopeptides	979:993	arg1	abundance					966:974	the abundance	962:974	the abundance of N-glycopeptides, the desialylation simplified the O-glycans	962:1037	The de-N-deglycosylation decreased the abundance of N-glycopeptides, the desialylation simplified the O-glycans and the enzymatic oxidization conferred the enrichment specificity.					
30350625	4	67	theme	strong	596:601	arg1	acid					603:606	strong acid	596:606	strong acid	596:606	In this approach, the tryptic digest of serum was first subjected to PNGase F treatment to release the N-glycan and was then treated with strong acid to release sialic acid residues from mucin-type O-glycans.					
31195299	0	0	theme	carotid	75:81	arg1	artery					83:88	the ovine carotid artery	65:88	the ovine carotid artery	65:88	MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery.					
31195299	4	1	theme	textile	718:724	arg1	mesh					726:729	the textile mesh	714:729	the textile mesh in the developing tissue	714:754	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	7	2	theme	USPIO	1019:1023	arg1	labeling					1025:1032	USPIO labeling	1019:1032	USPIO labeling	1019:1032	USPIO labeling neither induced calcifications, nor negatively affected their remodeling with respect to tissue-specific extracellular matrix composition and endothelialization.					
31195299	1	3	theme	employed	150:157	arg1	techniques					170:179	clinically employed monitoring techniques	139:179	clinically employed monitoring techniques	139:179	The modification of biomaterials to comply with clinically employed monitoring techniques is a promising strategy to support clinical translation in regenerative medicine.					
31195299	11	4	theme	surgical	1600:1607	arg1	interventions					1609:1621	further pharmacological or surgical interventions	1573:1621	further pharmacological or surgical interventions	1573:1621	Thereby, it can support clinical decision-making on the need for further pharmacological or surgical interventions.					
31195299	0	5	theme	ovine	69:73	arg1	artery					83:88	the ovine carotid artery	65:88	the ovine carotid artery	65:88	MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery.					
31195299	7	6	theme	tissue-specific	1123:1137	arg1	composition					1160:1170	tissue-specific extracellular matrix composition	1123:1170	tissue-specific extracellular matrix composition	1123:1170	USPIO labeling neither induced calcifications, nor negatively affected their remodeling with respect to tissue-specific extracellular matrix composition and endothelialization.					
31195299	1	7	theme	monitoring	159:168	arg1	techniques					170:179	clinically employed monitoring techniques	139:179	clinically employed monitoring techniques	139:179	The modification of biomaterials to comply with clinically employed monitoring techniques is a promising strategy to support clinical translation in regenerative medicine.					
31195299	11	8	theme	further	1573:1579	arg1	interventions					1609:1621	further pharmacological or surgical interventions	1573:1621	further pharmacological or surgical interventions	1573:1621	Thereby, it can support clinical decision-making on the need for further pharmacological or surgical interventions.					
31195299	0	9	from	MR	0:1	arg1	artery					83:88	the ovine carotid artery	65:88	the ovine carotid artery	65:88	MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery.					
31195299	2	10	theme	vascular	309:316	arg1	TEVG					326:329	TEVG	326:329	TEVG	326:329	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	2	10	theme	vascular	309:316	arg1	grafts					318:323	tissue-engineered vascular grafts	291:323	tissue-engineered vascular grafts (TEVG)	291:330	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	3	11	theme	longitudinal	591:602	arg1	evaluation					615:624	longitudinal functional evaluation	591:624	longitudinal functional evaluation in an ovine model for up to 8 weeks	591:660	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	6	12	theme	native	971:976	arg1	arteries					978:985	native arteries	971:985	native arteries	971:985	Contrary to unlabeled constructs, iTEVG could be delineated from native arteries and precisely localized by MRI.					
31195299	2	13	theme	tissue-engineered	291:307	arg1	TEVG					326:329	TEVG	326:329	TEVG	326:329	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	2	13	theme	tissue-engineered	291:307	arg1	grafts					318:323	tissue-engineered vascular grafts	291:323	tissue-engineered vascular grafts (TEVG)	291:330	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	2	14	theme	textile	367:373	arg1	scaffold					375:382	the textile scaffold	363:382	the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	363:449	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	4	15	from	mesh	726:729	arg1	tissue					749:754	the developing tissue	734:754	the developing tissue	734:754	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	10	16	theme	imaging	1443:1449	arg1	approaches					1451:1460	multimodal imaging approaches	1432:1460	multimodal imaging approaches	1432:1460	In conclusion, USPIO-labeling enables accurate localization of TEVG and opens up opportunities for multimodal imaging approaches to assess transplant acceptance and function.					
31195299	4	17	theme	complete	692:699	arg1	embedding					701:709	the complete embedding	688:709	the complete embedding of the textile mesh in the developing tissue	688:754	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	2	18	theme	oxide	423:427	arg1	nanoparticles					437:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	7	19	theme	matrix	1153:1158	arg1	composition					1160:1170	tissue-specific extracellular matrix composition	1123:1170	tissue-specific extracellular matrix composition	1123:1170	USPIO labeling neither induced calcifications, nor negatively affected their remodeling with respect to tissue-specific extracellular matrix composition and endothelialization.					
31195299	4	20	theme	7T-MRI	835:840	arg1	scanners					842:849	1T-, 3T- and 7T-MRI scanners	822:849	1T-, 3T- and 7T-MRI scanners	822:849	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	9	21	theme	18F-FDG	1243:1249	arg1	uptake					1251:1256	18F-FDG uptake	1243:1256	18F-FDG uptake (assessed via PET-CT)	1243:1278	18F-FDG uptake (assessed via PET-CT) indicated only transient post-surgical inflammation.					
31195299	3	22	theme	initial	564:570	arg1	control					580:586	initial quality control	564:586	initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks	564:660	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	4	23	from	embedding	701:709	arg1	tissue					749:754	the developing tissue	734:754	the developing tissue	734:754	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	2	24	theme	iron	418:421	arg1	nanoparticles					437:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	4	25	theme	Crucial	663:669	arg1	embedding					701:709	the complete embedding	688:709	the complete embedding of the textile mesh in the developing tissue	688:754	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	4	25	theme	Crucial	663:669	arg1	features					671:678	Crucial features	663:678	Crucial features such as the complete embedding of the textile mesh in the developing tissue	663:754	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	3	26	theme	functional	604:613	arg1	evaluation					615:624	longitudinal functional evaluation	591:624	longitudinal functional evaluation in an ovine model for up to 8 weeks	591:660	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	2	27	theme	superparamagnetic	400:416	arg1	nanoparticles					437:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	4	28	theme	structural	772:781	arg1	stability					783:791	the grafts' structural stability	760:791	the grafts' structural stability	760:791	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	2	29	theme	ultrasmall	389:398	arg1	nanoparticles					437:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	4	30	from	tissue	749:754	arg1	embedding					701:709	the complete embedding	688:709	the complete embedding of the textile mesh in the developing tissue	688:754	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	10	31	theme	accurate	1371:1378	arg1	localization					1380:1391	accurate localization	1371:1391	accurate localization of TEVG	1371:1399	In conclusion, USPIO-labeling enables accurate localization of TEVG and opens up opportunities for multimodal imaging approaches to assess transplant acceptance and function.					
31195299	0	32	theme	PET-CT	7:12	arg1	monitoring					14:23	PET-CT monitoring	7:23	PET-CT monitoring	7:23	MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery.					
31195299	10	33	theme	TEVG	1396:1399	arg1	localization					1380:1391	accurate localization	1371:1391	accurate localization of TEVG	1371:1399	In conclusion, USPIO-labeling enables accurate localization of TEVG and opens up opportunities for multimodal imaging approaches to assess transplant acceptance and function.					
31195299	2	34	theme	grafts	318:323	arg1	imaging					280:286	multimodal imaging	269:286	multimodal imaging of tissue-engineered vascular grafts (TEVG)	269:330	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	11	35	theme	clinical	1532:1539	arg1	decision-making					1541:1555	clinical decision-making	1532:1555	clinical decision-making	1532:1555	Thereby, it can support clinical decision-making on the need for further pharmacological or surgical interventions.					
31195299	0	36	theme	tissue-engineered	28:44	arg1	grafts					55:60	tissue-engineered vascular grafts	28:60	tissue-engineered vascular grafts	28:60	MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery.					
31195299	4	37	theme	mesh	726:729	arg1	embedding					701:709	the complete embedding	688:709	the complete embedding of the textile mesh in the developing tissue	688:754	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	3	38	theme	ovine	632:636	arg1	model					638:642	an ovine model	629:642	an ovine model for up to 8 weeks	629:660	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	1	39	theme	promising	186:194	arg1	strategy					196:203	a promising strategy	184:203	a promising strategy to support clinical translation in regenerative medicine	184:260	The modification of biomaterials to comply with clinically employed monitoring techniques is a promising strategy to support clinical translation in regenerative medicine.					
31195299	1	39	theme	promising	186:194	arg1	modification					95:106	The modification	91:106	The modification of biomaterials to comply with clinically employed monitoring techniques	91:179	The modification of biomaterials to comply with clinically employed monitoring techniques is a promising strategy to support clinical translation in regenerative medicine.					
31195299	2	40	theme	USPIO	430:434	arg1	nanoparticles					437:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	3	41	theme	resulting	456:464	arg1	iTEVG					487:491	iTEVG	487:491	iTEVG	487:491	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	3	41	theme	resulting	456:464	arg1	grafts					479:484	The resulting MR-imageable grafts	452:484	The resulting MR-imageable grafts (iTEVG)	452:492	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	4	42	theme	1T-	822:824	arg1	scanners					842:849	1T-, 3T- and 7T-MRI scanners	822:849	1T-, 3T- and 7T-MRI scanners	822:849	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	1	43	theme	clinical	216:223	arg1	translation					225:235	clinical translation	216:235	clinical translation	216:235	The modification of biomaterials to comply with clinically employed monitoring techniques is a promising strategy to support clinical translation in regenerative medicine.					
31195299	0	44	theme	grafts	55:60	arg1	MR					0:1	MR	0:1	MR	0:1	MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery.					
31195299	0	44	theme	grafts	55:60	arg1	monitoring					14:23	PET-CT monitoring	7:23	PET-CT monitoring	7:23	MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery.					
31195299	0	45	theme	vascular	46:53	arg1	grafts					55:60	tissue-engineered vascular grafts	28:60	tissue-engineered vascular grafts	28:60	MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery.					
31195299	6	46	theme	unlabeled	918:926	arg1	constructs					928:937	unlabeled constructs	918:937	unlabeled constructs	918:937	Contrary to unlabeled constructs, iTEVG could be delineated from native arteries and precisely localized by MRI.					
31195299	4	47	theme	3T-	827:829	arg1	scanners					842:849	1T-, 3T- and 7T-MRI scanners	822:849	1T-, 3T- and 7T-MRI scanners	822:849	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	3	48	theme	whole	541:545	arg1	life-cycle					547:556	their whole life-cycle	535:556	their whole life-cycle	535:556	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	1	49	theme	biomaterials	111:122	arg1	strategy					196:203	a promising strategy	184:203	a promising strategy to support clinical translation in regenerative medicine	184:260	The modification of biomaterials to comply with clinically employed monitoring techniques is a promising strategy to support clinical translation in regenerative medicine.					
31195299	1	49	theme	biomaterials	111:122	arg1	modification					95:106	The modification	91:106	The modification of biomaterials to comply with clinically employed monitoring techniques	91:179	The modification of biomaterials to comply with clinically employed monitoring techniques is a promising strategy to support clinical translation in regenerative medicine.					
31195299	10	50	theme	transplant	1472:1481	arg1	acceptance					1483:1492	transplant acceptance	1472:1492	transplant acceptance	1472:1492	In conclusion, USPIO-labeling enables accurate localization of TEVG and opens up opportunities for multimodal imaging approaches to assess transplant acceptance and function.					
31195299	9	51	theme	transient	1295:1303	arg1	inflammation					1319:1330	only transient post-surgical inflammation	1290:1330	only transient post-surgical inflammation	1290:1330	18F-FDG uptake (assessed via PET-CT) indicated only transient post-surgical inflammation.					
31195299	2	52	theme	multimodal	269:278	arg1	imaging					280:286	multimodal imaging	269:286	multimodal imaging of tissue-engineered vascular grafts (TEVG)	269:330	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	4	53	theme	developing	738:747	arg1	tissue					749:754	the developing tissue	734:754	the developing tissue	734:754	Crucial features such as the complete embedding of the textile mesh in the developing tissue and the grafts' structural stability were assessed in vitro using 1T-, 3T- and 7T-MRI scanners.					
31195299	3	54	from	evaluation	615:624	arg1	model					638:642	an ovine model	629:642	an ovine model for up to 8 weeks	629:660	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	9	55	theme	post-surgical	1305:1317	arg1	inflammation					1319:1330	only transient post-surgical inflammation	1290:1330	only transient post-surgical inflammation	1290:1330	18F-FDG uptake (assessed via PET-CT) indicated only transient post-surgical inflammation.					
31195299	3	56	theme	quality	572:578	arg1	control					580:586	initial quality control	564:586	initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks	564:660	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	10	57	theme	multimodal	1432:1441	arg1	approaches					1451:1460	multimodal imaging approaches	1432:1460	multimodal imaging approaches	1432:1460	In conclusion, USPIO-labeling enables accurate localization of TEVG and opens up opportunities for multimodal imaging approaches to assess transplant acceptance and function.					
31195299	3	58	dep	8	654:654	arg1	to					651:652	to	651:652	to	651:652	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	2	59	with	scaffold	375:382	arg1	nanoparticles					437:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles	389:449	Here, multimodal imaging of tissue-engineered vascular grafts (TEVG) was enabled by functionalizing the textile scaffold with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles.					
31195299	11	60	theme	pharmacological	1581:1595	arg1	interventions					1609:1621	further pharmacological or surgical interventions	1573:1621	further pharmacological or surgical interventions	1573:1621	Thereby, it can support clinical decision-making on the need for further pharmacological or surgical interventions.					
31195299	3	61	theme	MR-imageable	466:477	arg1	iTEVG					487:491	iTEVG	487:491	iTEVG	487:491	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	3	61	theme	MR-imageable	466:477	arg1	grafts					479:484	The resulting MR-imageable grafts	452:484	The resulting MR-imageable grafts (iTEVG)	452:492	The resulting MR-imageable grafts (iTEVG) were monitored non-invasively throughout their whole life-cycle, from initial quality control to longitudinal functional evaluation in an ovine model for up to 8 weeks.					
31195299	1	62	theme	regenerative	240:251	arg1	medicine					253:260	regenerative medicine	240:260	regenerative medicine	240:260	The modification of biomaterials to comply with clinically employed monitoring techniques is a promising strategy to support clinical translation in regenerative medicine.					
31195299	7	63	theme	extracellular	1139:1151	arg1	composition					1160:1170	tissue-specific extracellular matrix composition	1123:1170	tissue-specific extracellular matrix composition	1123:1170	USPIO labeling neither induced calcifications, nor negatively affected their remodeling with respect to tissue-specific extracellular matrix composition and endothelialization.					
31195299	0	64	from	monitoring	14:23	arg1	artery					83:88	the ovine carotid artery	65:88	the ovine carotid artery	65:88	MR and PET-CT monitoring of tissue-engineered vascular grafts in the ovine carotid artery.					
31669657	4	0	theme	observed	783:790	arg1	DMA					792:794	observed DMA	783:794	observed DMA	783:794	CS/PS foams exhibited a decrease in the storage modulus and glass transition temperatures as observed DMA.					
31669657	3	1	theme	dynamic	545:551	arg1	DMA					574:576	DMA	574:576	DMA	574:576	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	3	1	theme	dynamic	545:551	arg1	analysis					564:571	dynamic mechanical analysis	545:571	dynamic mechanical analysis (DMA)	545:577	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	1	2	theme	agro-industrial	135:149	arg1	skin					167:170	peanut skin	160:170	peanut skin	160:170	The present work reports the effects of adding an agro-industrial residue (peanut skin) to cassava starch-based foams developed by thermopressing process.					
31669657	1	2	theme	agro-industrial	135:149	arg1	residue					151:157	an agro-industrial residue	132:157	an agro-industrial residue (peanut skin)	132:171	The present work reports the effects of adding an agro-industrial residue (peanut skin) to cassava starch-based foams developed by thermopressing process.					
31669657	7	3	theme	peanut	1297:1302	arg1	skin					1304:1307	peanut skin	1297:1307	peanut skin	1297:1307	The reduction of hydrophilicity was related to the composition of the additive, mainly regarding lipids and proteins fractions, as well as to the interactions between cassava starch and peanut skin, reducing the availability of OH groups to bond with water.					
31669657	0	4	theme	biodegradable	64:76	arg1	foams					78:82	biodegradable foams	64:82	biodegradable foams	64:82	Study of interactions between cassava starch and peanut skin on biodegradable foams.					
31669657	3	5	theme	skin	609:612	arg1	addition					590:597	the addition	586:597	the addition of peanut skin	586:612	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	5	6	theme	peanut	813:818	arg1	skin					820:823	peanut skin	813:823	peanut skin	813:823	The addition of peanut skin did not influence on tensile stress and Young's modulus but reduced the tensile strain of the foams.					
31669657	0	7	from	Study	0:4	arg1	foams					78:82	biodegradable foams	64:82	biodegradable foams	64:82	Study of interactions between cassava starch and peanut skin on biodegradable foams.					
31669657	5	8	theme	foams	919:923	arg1	strain					905:910	the tensile strain	893:910	the tensile strain of the foams	893:923	The addition of peanut skin did not influence on tensile stress and Young's modulus but reduced the tensile strain of the foams.					
31669657	5	9	theme	Young	865:869	arg1	modulus					873:879	Young's modulus	865:879	Young's modulus	865:879	The addition of peanut skin did not influence on tensile stress and Young's modulus but reduced the tensile strain of the foams.					
31669657	6	10	theme	water	930:934	arg1	higher					968:973	higher	968:973	higher	968:973	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	6	10	theme	water	930:934	arg1	angle					944:948	The water contact angle	926:948	The water contact angle of CS/PS foam	926:962	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	2	11	theme	mechanical	447:456	arg1	structures					408:417	their chemical structures	393:417	their chemical structures	393:417	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	11	theme	mechanical	447:456	arg1	properties					458:467	thermal, morphological and mechanical properties	420:467	thermal, morphological and mechanical properties	420:467	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	6	12	theme	CS/PS	1006:1010	arg1	result					998:1003	a result	996:1003	a result	996:1003	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	6	12	theme	CS/PS	1006:1010	arg1	foams					1012:1016	CS/PS foams	1006:1016	CS/PS foams	1006:1016	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	7	13	theme	cassava	1278:1284	arg1	starch					1286:1291	cassava starch	1278:1291	cassava starch	1278:1291	The reduction of hydrophilicity was related to the composition of the additive, mainly regarding lipids and proteins fractions, as well as to the interactions between cassava starch and peanut skin, reducing the availability of OH groups to bond with water.					
31669657	5	14	theme	skin	820:823	arg1	addition					801:808	The addition	797:808	The addition of peanut skin	797:823	The addition of peanut skin did not influence on tensile stress and Young's modulus but reduced the tensile strain of the foams.					
31669657	4	15	theme	glass	750:754	arg1	transition					756:765	glass transition	750:765	glass transition	750:765	CS/PS foams exhibited a decrease in the storage modulus and glass transition temperatures as observed DMA.					
31669657	2	16	theme	skin	350:353	arg1	w/w					335:337	w/w	335:337	w/w	335:337	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	16	theme	skin	350:353	arg1	%					332:332	24%	330:332	24% (w/w) of peanut skin (CS/PS foams)	330:367	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	16	theme	skin	350:353	arg1	skin					350:353	peanut skin	343:353	peanut skin (CS/PS foams)	343:367	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	16	theme	skin	350:353	arg1	foams					362:366	CS/PS foams	356:366	CS/PS foams	356:366	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	4	17	theme	CS/PS	690:694	arg1	foams					696:700	CS/PS foams	690:700	CS/PS foams	690:700	CS/PS foams exhibited a decrease in the storage modulus and glass transition temperatures as observed DMA.					
31669657	6	18	theme	contact	936:942	arg1	higher					968:973	higher	968:973	higher	968:973	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	6	18	theme	contact	936:942	arg1	angle					944:948	The water contact angle	926:948	The water contact angle of CS/PS foam	926:962	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	2	19	theme	morphological	429:441	arg1	structures					408:417	their chemical structures	393:417	their chemical structures	393:417	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	19	theme	morphological	429:441	arg1	properties					458:467	thermal, morphological and mechanical properties	420:467	thermal, morphological and mechanical properties	420:467	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	6	20	theme	materials	1100:1108	arg1	application					1079:1089	the application	1075:1089	the application of these materials	1075:1108	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	1	21	theme	peanut	160:165	arg1	skin					167:170	peanut skin	160:170	peanut skin	160:170	The present work reports the effects of adding an agro-industrial residue (peanut skin) to cassava starch-based foams developed by thermopressing process.					
31669657	1	21	theme	peanut	160:165	arg1	residue					151:157	an agro-industrial residue	132:157	an agro-industrial residue (peanut skin)	132:171	The present work reports the effects of adding an agro-industrial residue (peanut skin) to cassava starch-based foams developed by thermopressing process.					
31669657	2	22	theme	CS	292:293	arg1	starch					284:289	cassava starch	276:289	cassava starch (CS foams)	276:300	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	22	theme	CS	292:293	arg1	foams					295:299	CS foams	292:299	CS foams	292:299	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	6	23	theme	CS/PS	953:957	arg1	foam					959:962	CS/PS foam	953:962	CS/PS foam	953:962	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	4	24	theme	transition	756:765	arg1	temperatures					767:778	the storage modulus and glass transition temperatures	726:778	the storage modulus and glass transition temperatures as observed DMA	726:794	CS/PS foams exhibited a decrease in the storage modulus and glass transition temperatures as observed DMA.					
31669657	5	25	theme	tensile	846:852	arg1	stress					854:859	tensile stress	846:859	tensile stress	846:859	The addition of peanut skin did not influence on tensile stress and Young's modulus but reduced the tensile strain of the foams.					
31669657	7	26	with	bond	1352:1355	arg1	water					1362:1366	water	1362:1366	water	1362:1366	The reduction of hydrophilicity was related to the composition of the additive, mainly regarding lipids and proteins fractions, as well as to the interactions between cassava starch and peanut skin, reducing the availability of OH groups to bond with water.					
31669657	6	27	theme	CS	1051:1052	arg1	foams					1054:1058	CS foams	1051:1058	CS foams	1051:1058	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	1	28	theme	present	89:95	arg1	work					97:100	The present work	85:100	The present work	85:100	The present work reports the effects of adding an agro-industrial residue (peanut skin) to cassava starch-based foams developed by thermopressing process.					
31669657	2	29	theme	CS/PS	356:360	arg1	skin					350:353	peanut skin	343:353	peanut skin (CS/PS foams)	343:367	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	29	theme	CS/PS	356:360	arg1	foams					362:366	CS/PS foams	356:366	CS/PS foams	356:366	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	30	theme	sorption	479:486	arg1	structures					408:417	their chemical structures	393:417	their chemical structures	393:417	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	30	theme	sorption	479:486	arg1	isotherms					488:496	moisture sorption isotherms	470:496	moisture sorption isotherms	470:496	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	31	theme	cassava	276:282	arg1	starch					284:289	cassava starch	276:289	cassava starch (CS foams)	276:300	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	31	theme	cassava	276:282	arg1	foams					295:299	CS foams	292:299	CS foams	292:299	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	1	32	theme	cassava	176:182	arg1	foams					197:201	cassava starch-based foams	176:201	cassava starch-based foams developed by thermopressing process	176:237	The present work reports the effects of adding an agro-industrial residue (peanut skin) to cassava starch-based foams developed by thermopressing process.					
31669657	2	33	theme	chemical	399:406	arg1	structures					408:417	their chemical structures	393:417	their chemical structures	393:417	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	33	theme	chemical	399:406	arg1	isotherms					488:496	moisture sorption isotherms	470:496	moisture sorption isotherms	470:496	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	33	theme	chemical	399:406	arg1	angle					507:511	contact angle	499:511	contact angle	499:511	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	33	theme	chemical	399:406	arg1	properties					458:467	thermal, morphological and mechanical properties	420:467	thermal, morphological and mechanical properties	420:467	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	3	34	theme	mobility	659:666	arg1	increase					647:654	the increase	643:654	the increase of mobility of the starch chains	643:687	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	2	35	theme	moisture	470:477	arg1	structures					408:417	their chemical structures	393:417	their chemical structures	393:417	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	35	theme	moisture	470:477	arg1	isotherms					488:496	moisture sorption isotherms	470:496	moisture sorption isotherms	470:496	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	1	36	theme	starch-based	184:195	arg1	foams					197:201	cassava starch-based foams	176:201	cassava starch-based foams developed by thermopressing process	176:237	The present work reports the effects of adding an agro-industrial residue (peanut skin) to cassava starch-based foams developed by thermopressing process.					
31669657	2	37	theme	peanut	343:348	arg1	skin					350:353	peanut skin	343:353	peanut skin (CS/PS foams)	343:367	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	37	theme	peanut	343:348	arg1	foams					362:366	CS/PS foams	356:366	CS/PS foams	356:366	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	0	38	theme	cassava	30:36	arg1	starch					38:43	cassava starch	30:43	cassava starch	30:43	Study of interactions between cassava starch and peanut skin on biodegradable foams.					
31669657	7	39	dep	lipids	1208:1213	arg1	fractions					1228:1236	fractions	1228:1236	fractions	1228:1236	The reduction of hydrophilicity was related to the composition of the additive, mainly regarding lipids and proteins fractions, as well as to the interactions between cassava starch and peanut skin, reducing the availability of OH groups to bond with water.					
31669657	0	40	theme	peanut	49:54	arg1	skin					56:59	peanut skin	49:59	peanut skin	49:59	Study of interactions between cassava starch and peanut skin on biodegradable foams.					
31669657	4	41	theme	modulus	738:744	arg1	temperatures					767:778	the storage modulus and glass transition temperatures	726:778	the storage modulus and glass transition temperatures as observed DMA	726:794	CS/PS foams exhibited a decrease in the storage modulus and glass transition temperatures as observed DMA.					
31669657	2	42	theme	cassava	306:312	arg1	starch					314:319	cassava starch	306:319	cassava starch	306:319	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	3	43	theme	starch	675:680	arg1	chains					682:687	the starch chains	671:687	the starch chains	671:687	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	7	44	theme	groups	1342:1347	arg1	availability					1323:1334	the availability	1319:1334	the availability of OH groups to bond with water	1319:1366	The reduction of hydrophilicity was related to the composition of the additive, mainly regarding lipids and proteins fractions, as well as to the interactions between cassava starch and peanut skin, reducing the availability of OH groups to bond with water.					
31669657	4	45	theme	storage	730:736	arg1	modulus					738:744	storage modulus	730:744	storage modulus	730:744	CS/PS foams exhibited a decrease in the storage modulus and glass transition temperatures as observed DMA.					
31669657	7	46	theme	additive	1181:1188	arg1	composition					1162:1172	the composition	1158:1172	the composition of the additive, mainly regarding lipids and proteins fractions	1158:1236	The reduction of hydrophilicity was related to the composition of the additive, mainly regarding lipids and proteins fractions, as well as to the interactions between cassava starch and peanut skin, reducing the availability of OH groups to bond with water.					
31669657	7	47	theme	hydrophilicity	1128:1141	arg1	reduction					1115:1123	The reduction	1111:1123	The reduction of hydrophilicity	1111:1141	The reduction of hydrophilicity was related to the composition of the additive, mainly regarding lipids and proteins fractions, as well as to the interactions between cassava starch and peanut skin, reducing the availability of OH groups to bond with water.					
31669657	7	47	theme	hydrophilicity	1128:1141	arg1	related					1147:1153	related	1147:1153	related	1147:1153	The reduction of hydrophilicity was related to the composition of the additive, mainly regarding lipids and proteins fractions, as well as to the interactions between cassava starch and peanut skin, reducing the availability of OH groups to bond with water.					
31669657	3	48	theme	chains	682:687	arg1	mobility					659:666	mobility	659:666	mobility of the starch chains	659:687	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	2	49	theme	contact	499:505	arg1	structures					408:417	their chemical structures	393:417	their chemical structures	393:417	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	49	theme	contact	499:505	arg1	angle					507:511	contact angle	499:511	contact angle	499:511	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	3	50	theme	peanut	602:607	arg1	skin					609:612	peanut skin	602:612	peanut skin	602:612	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	3	51	theme	mechanical	553:562	arg1	DMA					574:576	DMA	574:576	DMA	574:576	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	3	51	theme	mechanical	553:562	arg1	analysis					564:571	dynamic mechanical analysis	545:571	dynamic mechanical analysis (DMA)	545:577	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	3	52	theme	analysis	564:571	arg1	Results					534:540	Results	534:540	Results of dynamic mechanical analysis (DMA)	534:577	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
31669657	4	53	from	decrease	714:721	arg1	temperatures					767:778	the storage modulus and glass transition temperatures	726:778	the storage modulus and glass transition temperatures as observed DMA	726:794	CS/PS foams exhibited a decrease in the storage modulus and glass transition temperatures as observed DMA.					
31669657	7	54	theme	OH	1339:1340	arg1	groups					1342:1347	OH groups	1339:1347	OH groups	1339:1347	The reduction of hydrophilicity was related to the composition of the additive, mainly regarding lipids and proteins fractions, as well as to the interactions between cassava starch and peanut skin, reducing the availability of OH groups to bond with water.					
31669657	5	55	theme	tensile	897:903	arg1	strain					905:910	the tensile strain	893:910	the tensile strain of the foams	893:923	The addition of peanut skin did not influence on tensile stress and Young's modulus but reduced the tensile strain of the foams.					
31669657	6	56	theme	foam	959:962	arg1	higher					968:973	higher	968:973	higher	968:973	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	6	56	theme	foam	959:962	arg1	angle					944:948	The water contact angle	926:948	The water contact angle of CS/PS foam	926:962	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	2	57	theme	thermal	420:426	arg1	structures					408:417	their chemical structures	393:417	their chemical structures	393:417	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	2	57	theme	thermal	420:426	arg1	properties					458:467	thermal, morphological and mechanical properties	420:467	thermal, morphological and mechanical properties	420:467	For this purpose, foams composed of cassava starch (CS foams) and cassava starch added of 24% (w/w) of peanut skin (CS/PS foams) were compared regarding their chemical structures, thermal, morphological and mechanical properties, moisture sorption isotherms, contact angle, and biodegradation.					
31669657	0	58	theme	interactions	9:20	arg1	Study					0:4	Study	0:4	Study of interactions between cassava starch and peanut skin on biodegradable foams.	0:83	Study of interactions between cassava starch and peanut skin on biodegradable foams.					
31669657	6	59	theme	CS	980:981	arg1	foam					983:986	CS foam	980:986	CS foam	980:986	The water contact angle of CS/PS foam was higher than CS foam, and as a result, CS/PS foams were found less hydrophilic than CS foams, which broaden the application of these materials.					
31669657	3	60	dep	showed	579:584	arg1	reduced					614:620	reduced	614:620	showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains	579:687	Results of dynamic mechanical analysis (DMA) showed the addition of peanut skin reduced the stiffness due to the increase of mobility of the starch chains.					
30793484	7	0	theme	close	1308:1312	arg1	NBRC					1346:1349	the close related strain K. medellinensis NBRC 3288	1304:1354	the close related strain K. medellinensis NBRC 3288 unable to produce BC	1304:1375	Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed.					
30793484	6	1	theme	BC	1275:1276	arg1	synthesis					1262:1270	the synthesis	1258:1270	the synthesis of BC	1258:1276	qRT-PCR experiments demonstrated that mostly bcs1 and bcs4 are expressed under BC production conditions, suggesting that these operons direct the synthesis of BC.					
30793484	5	2	theme	polycistronic	1080:1092	arg1	mRNAs					1094:1098	four independent polycistronic mRNAs	1063:1098	four independent polycistronic mRNAs	1063:1098	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	3	3	from	bacterium	551:559	arg1	bases					514:518	the molecular bases	500:518	the molecular bases of the BC biosynthesis in this bacterium	500:559	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	1	4	theme	crystalline	275:285	arg1	BC					308:309	BC	308:309	BC	308:309	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	1	4	theme	crystalline	275:285	arg1	cellulose					297:305	crystalline bacterial cellulose	275:305	crystalline bacterial cellulose (BC)	275:310	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	7	5	theme	unable	1356:1361	arg1	NBRC					1346:1349	the close related strain K. medellinensis NBRC 3288	1304:1354	the close related strain K. medellinensis NBRC 3288 unable to produce BC	1304:1375	Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed.					
30793484	4	6	theme	genes	857:861	arg1	identification					831:844	the identification	827:844	the identification of the bcs genes involved in the cellulose biosynthesis	827:900	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	1	7	theme	Komagataeibacter	166:181	arg1	medellinensis					233:245	formerly Gluconacetobacter medellinensis ID13488	206:253	formerly Gluconacetobacter medellinensis ID13488	206:253	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	1	7	theme	Komagataeibacter	166:181	arg1	ID13488					197:203	Komagataeibacter medellinensis ID13488	166:203	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488)	166:254	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	1	8	theme	bacterial	287:295	arg1	BC					308:309	BC	308:309	BC	308:309	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	1	8	theme	bacterial	287:295	arg1	cellulose					297:305	crystalline bacterial cellulose	275:305	crystalline bacterial cellulose (BC)	275:310	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	7	9	theme	related	1314:1320	arg1	NBRC					1346:1349	the close related strain K. medellinensis NBRC 3288	1304:1354	the close related strain K. medellinensis NBRC 3288 unable to produce BC	1304:1375	Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed.					
30793484	4	10	with	analyses	731:738	arg1	strains					807:813	other cellulose-producing related strains	773:813	other cellulose-producing related strains	773:813	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	4	11	theme	Genome	713:718	arg1	analyses					731:738	Genome comparison analyses	713:738	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains	713:813	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	0	12	theme	nanocellulose	79:91	arg1	production					65:74	the production	61:74	the production of nanocellulose from the low pH resistant strain	61:124	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	3	13	from	biosynthesis	530:541	arg1	bacterium					551:559	this bacterium	546:559	this bacterium	546:559	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	3	14	theme	molecular	504:512	arg1	bases					514:518	the molecular bases	500:518	the molecular bases of the BC biosynthesis in this bacterium	500:559	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	0	15	theme	low	102:104	arg1	pH					106:107	low pH	102:107	the low pH resistant strain	98:124	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	4	16	theme	ID13488	760:766	arg1	analyses					731:738	Genome comparison analyses	713:738	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains	713:813	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	7	17	theme	K. medellinensis	1329:1344	arg1	NBRC					1346:1349	the close related strain K. medellinensis NBRC 3288	1304:1354	the close related strain K. medellinensis NBRC 3288 unable to produce BC	1304:1375	Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed.					
30793484	1	18	theme	medellinensis	183:195	arg1	medellinensis					233:245	formerly Gluconacetobacter medellinensis ID13488	206:253	formerly Gluconacetobacter medellinensis ID13488	206:253	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	1	18	theme	medellinensis	183:195	arg1	ID13488					197:203	Komagataeibacter medellinensis ID13488	166:203	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488)	166:254	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	5	19	theme	independent	1068:1078	arg1	mRNAs					1094:1098	four independent polycistronic mRNAs	1063:1098	four independent polycistronic mRNAs	1063:1098	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	20	dep	clusters	949:956	arg1	bcs4					980:983	bcs4	980:983	bcs4	980:983	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	20	dep	clusters	949:956	arg1	clusters					949:956	four bcs clusters	940:956	four bcs clusters (bcs1, bcs2, bcs3 and bcs4)	940:984	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	20	dep	clusters	949:956	arg1	bcs3					971:974	bcs3	971:974	bcs3	971:974	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	20	dep	clusters	949:956	arg1	bcs2					965:968	bcs2	965:968	bcs2	965:968	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	20	dep	clusters	949:956	arg1	bcs1					959:962	bcs1	959:962	bcs1	959:962	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	0	21	theme	Genome	0:5	arg1	sequence					7:14	Genome sequence	0:14	Genome sequence	0:14	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	7	22	theme	strain	1322:1327	arg1	NBRC					1346:1349	the close related strain K. medellinensis NBRC 3288	1304:1354	the close related strain K. medellinensis NBRC 3288 unable to produce BC	1304:1375	Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed.					
30793484	3	23	theme	Kb	701:702	arg1	plasmids					649:656	three putative plasmids	634:656	three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03)	634:710	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	4	24	theme	bcs	853:855	arg1	genes					857:861	the bcs genes	849:861	the bcs genes involved in the cellulose biosynthesis	849:900	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	2	25	theme	cider	470:474	arg1	production					476:485	cider production	470:485	cider production	470:485	These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated from cider production).					
30793484	3	26	from	bases	514:518	arg1	bacterium					551:559	this bacterium	546:559	this bacterium	546:559	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	5	27	theme	bcs	945:947	arg1	clusters					949:956	four bcs clusters	940:956	four bcs clusters (bcs1, bcs2, bcs3 and bcs4)	940:984	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	27	theme	bcs	945:947	arg1	bcs4					980:983	bcs4	980:983	bcs4	980:983	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	27	theme	bcs	945:947	arg1	bcs3					971:974	bcs3	971:974	bcs3	971:974	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	27	theme	bcs	945:947	arg1	bcs2					965:968	bcs2	965:968	bcs2	965:968	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	27	theme	bcs	945:947	arg1	bcs1					959:962	bcs1	959:962	bcs1	959:962	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	2	28	theme	BC	407:408	arg1	production					410:419	industrial BC production	396:419	industrial BC production from acidic residues (e.g. wastes generated from cider production)	396:486	These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated from cider production).					
30793484	4	29	theme	related	799:805	arg1	strains					807:813	other cellulose-producing related strains	773:813	other cellulose-producing related strains	773:813	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	0	30	theme	resistant	109:117	arg1	strain					119:124	the low pH resistant strain	98:124	the low pH resistant strain	98:124	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	4	31	theme	comparison	720:729	arg1	analyses					731:738	Genome comparison analyses	713:738	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains	713:813	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	1	32	theme	Gluconacetobacter	215:231	arg1	medellinensis					233:245	formerly Gluconacetobacter medellinensis ID13488	206:253	formerly Gluconacetobacter medellinensis ID13488	206:253	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	1	32	theme	Gluconacetobacter	215:231	arg1	ID13488					197:203	Komagataeibacter medellinensis ID13488	166:203	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488)	166:254	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	5	33	dep	Genes	903:907	arg1	Genes					903:907	Genes arrangement and composition	903:935	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4)	903:984	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	33	dep	Genes	903:907	arg1	composition					925:935	composition	925:935	composition	925:935	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	33	dep	Genes	903:907	arg1	arrangement					909:919	arrangement	909:919	arrangement	909:919	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	0	34	theme	pH	106:107	arg1	strain					119:124	the low pH resistant strain	98:124	the low pH resistant strain	98:124	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	3	35	theme	38.1 kb	661:667	arg1	plasmids					649:656	three putative plasmids	634:656	three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03)	634:710	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	3	36	theme	BC	527:528	arg1	biosynthesis					530:541	the BC biosynthesis	523:541	the BC biosynthesis in this bacterium	523:559	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	3	37	theme	biosynthesis	530:541	arg1	bases					514:518	the molecular bases	500:518	the molecular bases of the BC biosynthesis in this bacterium	500:559	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	1	38	theme	high	318:321	arg1	conditions					337:346	high acidic growth conditions	318:346	high acidic growth conditions	318:346	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	7	39	with	differences	1287:1297	arg1	NBRC					1346:1349	the close related strain K. medellinensis NBRC 3288	1304:1354	the close related strain K. medellinensis NBRC 3288 unable to produce BC	1304:1375	Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed.					
30793484	1	40	theme	formerly	206:213	arg1	medellinensis					233:245	formerly Gluconacetobacter medellinensis ID13488	206:253	formerly Gluconacetobacter medellinensis ID13488	206:253	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	1	40	theme	formerly	206:213	arg1	ID13488					197:203	Komagataeibacter medellinensis ID13488	166:203	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488)	166:254	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	2	41	theme	acidic	426:431	arg1	residues					433:440	acidic residues	426:440	acidic residues (e.g. wastes generated from cider production)	426:486	These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated from cider production).					
30793484	1	42	theme	acidic	323:328	arg1	conditions					337:346	high acidic growth conditions	318:346	high acidic growth conditions	318:346	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	2	43	dep	residues	433:440	arg1	wastes					448:453	e.g. wastes	443:453	e.g. wastes generated from cider production	443:485	These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated from cider production).					
30793484	7	44	theme	Genomic	1279:1285	arg1	differences					1287:1297	Genomic differences	1279:1297	Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC	1279:1375	Genomic differences with the close related strain K. medellinensis NBRC 3288 unable to produce BC were also described and discussed.					
30793484	0	45	theme	clusters	48:55	arg1	characterization					20:35	characterization	20:35	characterization	20:35	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	0	45	theme	clusters	48:55	arg1	sequence					7:14	Genome sequence	0:14	Genome sequence	0:14	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	1	46	theme	growth	330:335	arg1	conditions					337:346	high acidic growth conditions	318:346	high acidic growth conditions	318:346	Komagataeibacter medellinensis ID13488 (formerly Gluconacetobacter medellinensis ID13488) is able to produce crystalline bacterial cellulose (BC) under high acidic growth conditions.					
30793484	0	47	theme	bcs	44:46	arg1	clusters					48:55	the bcs clusters	40:55	the bcs clusters	40:55	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	4	48	theme	cellulose	879:887	arg1	biosynthesis					889:900	the cellulose biosynthesis	875:900	the cellulose biosynthesis	875:900	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	6	49	theme	production	1198:1207	arg1	conditions					1209:1218	BC production conditions	1195:1218	BC production conditions	1195:1218	qRT-PCR experiments demonstrated that mostly bcs1 and bcs4 are expressed under BC production conditions, suggesting that these operons direct the synthesis of BC.					
30793484	3	50	theme	4.3 kb	678:683	arg1	plasmids					649:656	three putative plasmids	634:656	three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03)	634:710	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	4	51	theme	other	773:777	arg1	strains					807:813	other cellulose-producing related strains	773:813	other cellulose-producing related strains	773:813	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	5	52	theme	clusters	949:956	arg1	Genes					903:907	Genes arrangement and composition	903:935	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4)	903:984	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	52	theme	clusters	949:956	arg1	composition					925:935	composition	925:935	composition	925:935	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	5	52	theme	clusters	949:956	arg1	arrangement					909:919	arrangement	909:919	arrangement	909:919	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	3	53	contain	containing	623:632	arg2	plasmids					649:656	three putative plasmids	634:656	three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03)	634:710	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	3	53	contain	containing	623:632	arg1	3.4 Mb					616:621	3.4 Mb	616:621	3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03)	616:710	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	4	54	theme	K. medellinensis	743:758	arg1	ID13488					760:766	K. medellinensis ID13488	743:766	K. medellinensis ID13488	743:766	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	3	55	theme	putative	640:647	arg1	plasmids					649:656	three putative plasmids	634:656	three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03)	634:710	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	0	56	dep	medellinensis	143:155	arg1	characterization					20:35	characterization	20:35	characterization	20:35	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	0	56	dep	medellinensis	143:155	arg1	sequence					7:14	Genome sequence	0:14	Genome sequence	0:14	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	0	57	from	strain	119:124	arg1	production					65:74	the production	61:74	the production of nanocellulose from the low pH resistant strain	61:124	Genome sequence and characterization of the bcs clusters for the production of nanocellulose from the low pH resistant strain Komagataeibacter medellinensis ID13488.					
30793484	3	58	theme	3.4 Mb	616:621	arg1	sequence					604:611	a sequence	602:611	a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03)	602:710	To explore the molecular bases of the BC biosynthesis in this bacterium, the genome has been sequenced revealing a sequence of 3.4 Mb containing three putative plasmids of 38.1 kb (pKM01), 4.3 kb (pKM02) and 3.3 Kb (pKM03).					
30793484	2	59	from	residues	433:440	arg1	production					410:419	industrial BC production	396:419	industrial BC production from acidic residues (e.g. wastes generated from cider production)	396:486	These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated from cider production).					
30793484	2	60	theme	industrial	396:405	arg1	production					410:419	industrial BC production	396:419	industrial BC production from acidic residues (e.g. wastes generated from cider production)	396:486	These abilities make this strain desirable for industrial BC production from acidic residues (e.g. wastes generated from cider production).					
30793484	5	61	from	organization	1019:1030	arg1	operons					1040:1046	four operons	1035:1046	four operons transcribed as four independent polycistronic mRNAs	1035:1098	Genes arrangement and composition of four bcs clusters (bcs1, bcs2, bcs3 and bcs4) was studied by RT-PCR, and their organization in four operons transcribed as four independent polycistronic mRNAs was determined.					
30793484	6	62	theme	qRT-PCR	1116:1122	arg1	experiments					1124:1134	qRT-PCR experiments	1116:1134	qRT-PCR experiments	1116:1134	qRT-PCR experiments demonstrated that mostly bcs1 and bcs4 are expressed under BC production conditions, suggesting that these operons direct the synthesis of BC.					
30793484	4	63	theme	cellulose-producing	779:797	arg1	strains					807:813	other cellulose-producing related strains	773:813	other cellulose-producing related strains	773:813	Genome comparison analyses of K. medellinensis ID13488 with other cellulose-producing related strains resulted in the identification of the bcs genes involved in the cellulose biosynthesis.					
30793484	6	64	theme	BC	1195:1196	arg1	conditions					1209:1218	BC production conditions	1195:1218	BC production conditions	1195:1218	qRT-PCR experiments demonstrated that mostly bcs1 and bcs4 are expressed under BC production conditions, suggesting that these operons direct the synthesis of BC.					
30176524	7	0	theme	chemical	1157:1164	arg1	stability					1166:1174	the chemical stability	1153:1174	the chemical stability of the systems	1153:1189	Indeed, no drug content decaying was observed, which corroborates the chemical stability of the systems as indicated by thermogravimetry, chromatographic, morphological and spectroscopic assays.					
30176524	7	1	theme	systems	1183:1189	arg1	stability					1166:1174	the chemical stability	1153:1174	the chemical stability of the systems	1153:1189	Indeed, no drug content decaying was observed, which corroborates the chemical stability of the systems as indicated by thermogravimetry, chromatographic, morphological and spectroscopic assays.					
30176524	4	2	theme	selected	539:546	arg1	excipients					548:557	The selected excipients	535:557	The selected excipients	535:557	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	9	3	theme	better	1617:1622	arg1	results					1624:1630	better results	1617:1630	better results in terms of stability and drug delivery	1617:1670	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	6	4	theme	thermal	1025:1031	arg1	treatment					1033:1041	a thermal treatment	1023:1041	a thermal treatment used to simulate pharmaceutical processing	1023:1084	The stability of samples was preserved even after a thermal treatment used to simulate pharmaceutical processing.					
30176524	8	5	theme	form	1339:1342	arg1	drug					1320:1323	the drug	1316:1323	the drug (orthorhombic form I)	1316:1345	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	8	5	theme	form	1339:1342	arg1	I					1344:1344	orthorhombic form I	1326:1344	orthorhombic form I	1326:1344	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	9	6	theme	components	1594:1603	arg1	proportions					1579:1589	proportions	1579:1589	proportions of components	1579:1603	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	9	6	theme	components	1594:1603	arg1	combinations					1562:1573	combinations	1562:1573	combinations	1562:1573	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	9	7	theme	FIN	1543:1545	arg1	use					1536:1538	topical use	1528:1538	topical use of FIN	1528:1545	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	3	8	theme	drug	503:506	arg1	stability					486:494	the stability	482:494	the stability of the drug in the different mixtures	482:532	An extensive analytical arsenal was used to encompass the stability of the drug in the different mixtures.					
30176524	5	9	theme	inclusion	900:908	arg1	complexes					910:918	inclusion complexes	900:918	inclusion complexes with HPβCD	900:929	The mixture design allowed measuring the interactions between components, showing that KLU enhanced the ability of the drug to form inclusion complexes with HPβCD, while SOL exhibited the opposite effect.					
30176524	5	10	theme	drug	887:890	arg1	ability					872:878	the ability	868:878	the ability of the drug to form inclusion complexes with HPβCD	868:929	The mixture design allowed measuring the interactions between components, showing that KLU enhanced the ability of the drug to form inclusion complexes with HPβCD, while SOL exhibited the opposite effect.					
30176524	9	11	theme	topical	1528:1534	arg1	use					1536:1538	topical use	1528:1538	topical use of FIN	1528:1545	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	0	12	theme	Preformulation	0:13	arg1	studies					15:21	Preformulation studies	0:21	Preformulation studies of finasteride	0:36	Preformulation studies of finasteride to design matrix systems for topical delivery.					
30176524	3	13	theme	extensive	431:439	arg1	arsenal					452:458	An extensive analytical arsenal	428:458	An extensive analytical arsenal	428:458	An extensive analytical arsenal was used to encompass the stability of the drug in the different mixtures.					
30176524	8	14	theme	drug	1320:1323	arg1	phase					1307:1311	The original crystalline phase	1282:1311	The original crystalline phase of the drug (orthorhombic form I)	1282:1345	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	6	15	theme	samples	990:996	arg1	stability					977:985	The stability	973:985	The stability of samples	973:996	The stability of samples was preserved even after a thermal treatment used to simulate pharmaceutical processing.					
30176524	6	16	theme	pharmaceutical	1060:1073	arg1	processing					1075:1084	pharmaceutical processing	1060:1084	pharmaceutical processing	1060:1084	The stability of samples was preserved even after a thermal treatment used to simulate pharmaceutical processing.					
30176524	2	17	theme	drug	253:256	arg1	compatibility					232:244	The compatibility	228:244	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL)	228:363	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	1	18	with	study	102:106	arg1	FIN					126:128	FIN	126:128	FIN	126:128	A preformulation study with finasteride (FIN) was conducted to enable the development of a topical matrix system to treat androgenic alopecia.					
30176524	1	18	with	study	102:106	arg1	finasteride					113:123	finasteride	113:123	finasteride (FIN)	113:129	A preformulation study with finasteride (FIN) was conducted to enable the development of a topical matrix system to treat androgenic alopecia.					
30176524	9	19	theme	experiments	1466:1476	arg1	series					1456:1461	these series	1450:1461	these series of experiments	1450:1476	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	2	20	dep	polymers	320:327	arg1	KLU					341:343	KLU	341:343	KLU	341:343	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	20	dep	polymers	320:327	arg1	Soluplus					350:357	Soluplus	350:357	Soluplus	350:357	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	20	dep	polymers	320:327	arg1	EXF					336:338	EXF	336:338	EXF	336:338	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	20	dep	polymers	320:327	arg1	polymers					320:327	the hydrophilic polymers	304:327	the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL)	304:363	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	21	theme	centroid	403:410	arg1	design					420:425	a simplex centroid mixture design	393:425	a simplex centroid mixture design	393:425	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	9	22	theme	delivery	1507:1514	arg1	systems					1516:1522	delivery systems	1507:1522	delivery systems	1507:1522	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	8	23	theme	physical	1424:1431	arg1	stability					1433:1441	its physical stability	1420:1441	its physical stability	1420:1441	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	1	24	theme	preformulation	87:100	arg1	study					102:106	A preformulation study	85:106	A preformulation study with finasteride (FIN)	85:129	A preformulation study with finasteride (FIN) was conducted to enable the development of a topical matrix system to treat androgenic alopecia.					
30176524	0	25	theme	finasteride	26:36	arg1	studies					15:21	Preformulation studies	0:21	Preformulation studies of finasteride	0:36	Preformulation studies of finasteride to design matrix systems for topical delivery.					
30176524	9	26	from	results	1624:1630	arg1	terms					1635:1639	terms	1635:1639	terms of stability and drug delivery	1635:1670	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	3	27	from	stability	486:494	arg1	mixtures					525:532	the different mixtures	511:532	the different mixtures	511:532	An extensive analytical arsenal was used to encompass the stability of the drug in the different mixtures.					
30176524	4	28	theme	reduced	695:701	arg1	temperatures					703:714	reduced temperatures	695:714	reduced temperatures	695:714	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	1	29	theme	topical	176:182	arg1	system					191:196	a topical matrix system	174:196	a topical matrix system to treat androgenic alopecia	174:225	A preformulation study with finasteride (FIN) was conducted to enable the development of a topical matrix system to treat androgenic alopecia.					
30176524	4	30	theme	melting	679:685	arg1	peak					687:690	FIN melting peak	675:690	FIN melting peak to reduced temperatures	675:714	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	3	31	theme	different	515:523	arg1	mixtures					525:532	the different mixtures	511:532	the different mixtures	511:532	An extensive analytical arsenal was used to encompass the stability of the drug in the different mixtures.					
30176524	1	32	theme	matrix	184:189	arg1	system					191:196	a topical matrix system	174:196	a topical matrix system to treat androgenic alopecia	174:225	A preformulation study with finasteride (FIN) was conducted to enable the development of a topical matrix system to treat androgenic alopecia.					
30176524	4	33	theme	sample	658:663	arg1	composition					639:649	the composition	635:649	the composition of the sample	635:663	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	7	34	theme	morphological	1242:1254	arg1	assays					1274:1279	thermogravimetry, chromatographic, morphological and spectroscopic assays	1207:1279	thermogravimetry, chromatographic, morphological and spectroscopic assays	1207:1279	Indeed, no drug content decaying was observed, which corroborates the chemical stability of the systems as indicated by thermogravimetry, chromatographic, morphological and spectroscopic assays.					
30176524	5	35	theme	opposite	956:963	arg1	effect					965:970	the opposite effect	952:970	the opposite effect	952:970	The mixture design allowed measuring the interactions between components, showing that KLU enhanced the ability of the drug to form inclusion complexes with HPβCD, while SOL exhibited the opposite effect.					
30176524	1	36	theme	system	191:196	arg1	development					159:169	the development	155:169	the development of a topical matrix system to treat androgenic alopecia	155:225	A preformulation study with finasteride (FIN) was conducted to enable the development of a topical matrix system to treat androgenic alopecia.					
30176524	7	37	theme	drug	1098:1101	arg1	content					1103:1109	no drug content	1095:1109	no drug content decaying	1095:1118	Indeed, no drug content decaying was observed, which corroborates the chemical stability of the systems as indicated by thermogravimetry, chromatographic, morphological and spectroscopic assays.					
30176524	2	38	with	compatibility	232:244	arg1	SOL					360:362	SOL	360:362	SOL	360:362	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	38	with	compatibility	232:244	arg1	hidroxypropyl-β-cyclodextrin					263:290	hidroxypropyl-β-cyclodextrin	263:290	hidroxypropyl-β-cyclodextrin (HPβCD)	263:298	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	38	with	compatibility	232:244	arg1	polymers					320:327	the hydrophilic polymers	304:327	the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL)	304:363	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	38	with	compatibility	232:244	arg1	Soluplus					350:357	Soluplus	350:357	Soluplus	350:357	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	38	with	compatibility	232:244	arg1	EXF					336:338	EXF	336:338	EXF	336:338	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	38	with	compatibility	232:244	arg1	HPβCD					293:297	HPβCD	293:297	HPβCD	293:297	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	9	39	theme	stability	1644:1652	arg1	terms					1635:1639	terms	1635:1639	terms of stability and drug delivery	1635:1670	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	2	40	theme	simplex	395:401	arg1	design					420:425	a simplex centroid mixture design	393:425	a simplex centroid mixture design	393:425	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	5	41	with	complexes	910:918	arg1	HPβCD					925:929	HPβCD	925:929	HPβCD	925:929	The mixture design allowed measuring the interactions between components, showing that KLU enhanced the ability of the drug to form inclusion complexes with HPβCD, while SOL exhibited the opposite effect.					
30176524	8	42	theme	orthorhombic	1326:1337	arg1	drug					1320:1323	the drug	1316:1323	the drug (orthorhombic form I)	1316:1345	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	8	42	theme	orthorhombic	1326:1337	arg1	I					1344:1344	orthorhombic form I	1326:1344	orthorhombic form I	1326:1344	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	8	43	theme	samples	1397:1403	arg1	treatment					1380:1388	the heating treatment	1368:1388	the heating treatment of the samples	1368:1403	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	3	44	used	used	464:467	arg2	arsenal					452:458	An extensive analytical arsenal	428:458	An extensive analytical arsenal	428:458	An extensive analytical arsenal was used to encompass the stability of the drug in the different mixtures.					
30176524	7	45	theme	chromatographic	1225:1239	arg1	assays					1274:1279	thermogravimetry, chromatographic, morphological and spectroscopic assays	1207:1279	thermogravimetry, chromatographic, morphological and spectroscopic assays	1207:1279	Indeed, no drug content decaying was observed, which corroborates the chemical stability of the systems as indicated by thermogravimetry, chromatographic, morphological and spectroscopic assays.					
30176524	0	46	theme	matrix	48:53	arg1	systems					55:61	matrix systems	48:61	matrix systems for topical delivery	48:82	Preformulation studies of finasteride to design matrix systems for topical delivery.					
30176524	9	47	theme	drug	1658:1661	arg1	delivery					1663:1670	drug delivery	1658:1670	drug delivery	1658:1670	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	8	48	theme	original	1286:1293	arg1	phase					1307:1311	The original crystalline phase	1282:1311	The original crystalline phase of the drug (orthorhombic form I)	1282:1345	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	4	49	theme	FIN	675:677	arg1	peak					687:690	FIN melting peak	675:690	FIN melting peak to reduced temperatures	675:714	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	8	50	theme	heating	1372:1378	arg1	treatment					1380:1388	the heating treatment	1368:1388	the heating treatment of the samples	1368:1403	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	9	51	theme	delivery	1663:1670	arg1	terms					1635:1639	terms	1635:1639	terms of stability and drug delivery	1635:1670	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	4	52	theme	enthalpy	758:765	arg1	decrease					731:738	the decrease	727:738	the decrease of its associated enthalpy	727:765	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	3	53	theme	analytical	441:450	arg1	arsenal					452:458	An extensive analytical arsenal	428:458	An extensive analytical arsenal	428:458	An extensive analytical arsenal was used to encompass the stability of the drug in the different mixtures.					
30176524	5	54	theme	mixture	772:778	arg1	design					780:785	The mixture design	768:785	The mixture design	768:785	The mixture design allowed measuring the interactions between components, showing that KLU enhanced the ability of the drug to form inclusion complexes with HPβCD, while SOL exhibited the opposite effect.					
30176524	2	55	theme	hydrophilic	308:318	arg1	SOL					360:362	SOL	360:362	SOL	360:362	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	55	theme	hydrophilic	308:318	arg1	Soluplus					350:357	Soluplus	350:357	Soluplus	350:357	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	55	theme	hydrophilic	308:318	arg1	EXF					336:338	EXF	336:338	EXF	336:338	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	2	55	theme	hydrophilic	308:318	arg1	polymers					320:327	the hydrophilic polymers	304:327	the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL)	304:363	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	8	56	theme	crystalline	1295:1305	arg1	phase					1307:1311	The original crystalline phase	1282:1311	The original crystalline phase of the drug (orthorhombic form I)	1282:1345	The original crystalline phase of the drug (orthorhombic form I) did not change after the heating treatment of the samples, demonstrating its physical stability.					
30176524	1	57	theme	androgenic	207:216	arg1	alopecia					218:225	androgenic alopecia	207:225	androgenic alopecia	207:225	A preformulation study with finasteride (FIN) was conducted to enable the development of a topical matrix system to treat androgenic alopecia.					
30176524	0	58	theme	topical	67:73	arg1	delivery					75:82	topical delivery	67:82	topical delivery	67:82	Preformulation studies of finasteride to design matrix systems for topical delivery.					
30176524	4	59	with	interaction	590:600	arg1	FIN					607:609	FIN	607:609	FIN	607:609	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	4	60	theme	associated	747:756	arg1	enthalpy					758:765	its associated enthalpy	743:765	its associated enthalpy	743:765	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	7	61	theme	spectroscopic	1260:1272	arg1	assays					1274:1279	thermogravimetry, chromatographic, morphological and spectroscopic assays	1207:1279	thermogravimetry, chromatographic, morphological and spectroscopic assays	1207:1279	Indeed, no drug content decaying was observed, which corroborates the chemical stability of the systems as indicated by thermogravimetry, chromatographic, morphological and spectroscopic assays.					
30176524	9	62	theme	systems	1516:1522	arg1	development					1492:1502	the development	1488:1502	the development of delivery systems	1488:1522	Thus, these series of experiments may guide the development of delivery systems for topical use of FIN, showing which combinations and proportions of components can lead to better results in terms of stability and drug delivery.					
30176524	4	63	theme	thermal	582:588	arg1	interaction					590:600	intense thermal interaction	574:600	intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy	574:765	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	4	64	contain	have	569:572	arg1	excipients					548:557	The selected excipients	535:557	The selected excipients	535:557	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	4	64	contain	have	569:572	arg2	interaction					590:600	intense thermal interaction	574:600	intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy	574:765	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30176524	7	65	theme	thermogravimetry	1207:1222	arg1	assays					1274:1279	thermogravimetry, chromatographic, morphological and spectroscopic assays	1207:1279	thermogravimetry, chromatographic, morphological and spectroscopic assays	1207:1279	Indeed, no drug content decaying was observed, which corroborates the chemical stability of the systems as indicated by thermogravimetry, chromatographic, morphological and spectroscopic assays.					
30176524	2	66	theme	mixture	412:418	arg1	design					420:425	a simplex centroid mixture design	393:425	a simplex centroid mixture design	393:425	The compatibility of the drug with hidroxypropyl-β-cyclodextrin (HPβCD) and the hydrophilic polymers Klucel EXF (KLU) and Soluplus (SOL) were evaluated according to a simplex centroid mixture design.					
30176524	4	67	theme	intense	574:580	arg1	interaction					590:600	intense thermal interaction	574:600	intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy	574:765	The selected excipients showed to have intense thermal interaction with FIN, which was dependent on the composition of the sample, shifting FIN melting peak to reduced temperatures along with the decrease of its associated enthalpy.					
30921126	5	0	theme	hyaluronic	896:905	arg1	acid					907:910	hyaluronic acid	896:910	hyaluronic acid	896:910	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	9	1	theme	Group	1566:1570	arg1	A					1572:1572	Group A	1566:1572	Group A	1566:1572	Group A differed significantly from groups B, C, D, and E (p < 0.001).					
30921126	0	2	theme	Clinicopathologic	71:87	arg1	Observations					89:100	Clinicopathologic Observations	71:100	Clinicopathologic Observations	71:100	Experimentally Induced Arterial Embolism by Hyaluronic Acid Injection: Clinicopathologic Observations and Treatment.					
30921126	5	3	theme	second	760:765	arg1	part					767:770	the second part	756:770	the second part of the study	756:783	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	3	4	theme	animal	606:611	arg1	model					613:617	an animal model	603:617	an animal model	603:617	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	4	5	theme	epigastric	693:702	arg1	artery					704:709	The inferior epigastric artery	680:709	The inferior epigastric artery	680:709	The inferior epigastric artery was retrieved for pathologic examination.					
30921126	1	6	theme	procedures	235:244	arg1	number					225:230	the rapidly growing number	205:230	the rapidly growing number of procedures performed and their expanding applications	205:287	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	11	7	theme	subcutaneous	1758:1769	arg1	hyaluronidase					1771:1783	intravenous or subcutaneous hyaluronidase	1743:1783	intravenous or subcutaneous hyaluronidase	1743:1783	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	8	8	theme	survival	1321:1328	arg1	rates					1330:1334	flap survival rates	1316:1334	flap survival rates (mean percentages)	1316:1353	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	8	theme	survival	1321:1328	arg1	percentages					1342:1352	mean percentages	1337:1352	mean percentages	1337:1352	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	1	9	theme	hyaluronic	160:169	arg1	injection					176:184	hyaluronic acid injection	160:184	hyaluronic acid injection	160:184	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	1	10	dep	BACKGROUND	117:126	arg1	occur					193:197	occur	193:197	occur	193:197	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	9	11	dep	groups	1602:1607	arg1	D					1615:1615	D	1615:1615	D	1615:1615	Group A differed significantly from groups B, C, D, and E (p < 0.001).					
30921126	9	11	dep	groups	1602:1607	arg1	E					1622:1622	E	1622:1622	E	1622:1622	Group A differed significantly from groups B, C, D, and E (p < 0.001).					
30921126	9	11	dep	groups	1602:1607	arg1	C					1612:1612	C	1612:1612	C	1612:1612	Group A differed significantly from groups B, C, D, and E (p < 0.001).					
30921126	9	11	dep	groups	1602:1607	arg1	groups					1602:1607	groups B, C, D, and E	1602:1622	groups B, C, D, and E	1602:1622	Group A differed significantly from groups B, C, D, and E (p < 0.001).					
30921126	9	11	dep	groups	1602:1607	arg1	B					1609:1609	B	1609:1609	B	1609:1609	Group A differed significantly from groups B, C, D, and E (p < 0.001).					
30921126	0	12	theme	Arterial	23:30	arg1	Embolism					32:39	Experimentally Induced Arterial Embolism	0:39	Experimentally Induced Arterial Embolism by Hyaluronic Acid Injection: Clinicopathologic Observations and Treatment.	0:115	Experimentally Induced Arterial Embolism by Hyaluronic Acid Injection: Clinicopathologic Observations and Treatment.					
30921126	1	13	theme	injection	176:184	arg1	complications					143:155	major complications	137:155	major complications of hyaluronic acid injection	137:184	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	8	14	theme	59.27	1444:1448	arg1	percent					1458:1464	59.27 ± 13.40 percent	1444:1464	59.27 ± 13.40 percent	1444:1464	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	14	theme	59.27	1444:1448	arg1	C					1441:1441	group C	1435:1441	group C	1435:1441	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	5	15	theme	right	934:938	arg1	side					940:943	the right side	930:943	the right side	930:943	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	5	16	theme	study	779:783	arg1	part					767:770	the second part	756:770	the second part of the study	756:783	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	6	17	theme	survival	1007:1014	arg1	rate					1016:1019	The flap survival rate	998:1019	The flap survival rate	998:1019	The flap survival rate was calculated and analyzed.					
30921126	11	18	theme	intravenous	1743:1753	arg1	hyaluronidase					1771:1783	intravenous or subcutaneous hyaluronidase	1743:1783	intravenous or subcutaneous hyaluronidase	1743:1783	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	3	19	theme	study	477:481	arg1	part					465:468	the first part	455:468	the first part of the study	455:481	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	5	20	theme	bilateral	786:794	arg1	flaps					806:810	bilateral abdominal flaps	786:810	bilateral abdominal flaps supplied by the inferior epigastric artery	786:853	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	7	21	theme	hyaluronic	1249:1258	arg1	mixture					1274:1280	a hyaluronic acid-thrombus mixture	1247:1280	a hyaluronic acid-thrombus mixture	1247:1280	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	8	22	theme	group	1372:1376	arg1	A					1378:1378	group A	1372:1378	group A	1372:1378	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	22	theme	group	1372:1376	arg1	percent					1557:1563	57.47 ± 13.64 percent	1543:1563	57.47 ± 13.64 percent	1543:1563	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	7	23	dep	RESULTS	1050:1056	arg1	revealed					1113:1120	revealed	1113:1120	revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture	1113:1280	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	7	24	theme	acid-induced	1187:1198	arg1	occlusion					1200:1208	arterial hyaluronic acid-induced occlusion	1167:1208	arterial hyaluronic acid-induced occlusion	1167:1208	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	5	25	theme	different	974:982	arg1	intervention					984:995	a different intervention	972:995	a different intervention	972:995	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	3	26	theme	first	459:463	arg1	part					465:468	the first part	455:468	the first part of the study	455:481	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	8	27	theme	±	1387:1387	arg1	B					1409:1409	43.29 ± 9.28 percent; group B	1381:1409	43.29 ± 9.28 percent; group B	1381:1409	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	11	28	theme	combined	1727:1734	arg1	use					1736:1738	The combined use	1723:1738	The combined use of intravenous or subcutaneous hyaluronidase	1723:1783	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	5	29	theme	inferior	828:835	arg1	artery					848:853	the inferior epigastric artery	824:853	the inferior epigastric artery	824:853	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	7	30	theme	arterial	1167:1174	arg1	occlusion					1200:1208	arterial hyaluronic acid-induced occlusion	1167:1208	arterial hyaluronic acid-induced occlusion	1167:1208	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	7	31	theme	acid-thrombus	1260:1272	arg1	mixture					1274:1280	a hyaluronic acid-thrombus mixture	1247:1280	a hyaluronic acid-thrombus mixture	1247:1280	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	8	32	theme	percent	1394:1400	arg1	B					1409:1409	43.29 ± 9.28 percent; group B	1381:1409	43.29 ± 9.28 percent; group B	1381:1409	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	2	33	theme	treatment	378:386	arg1	methods					388:394	optimal treatment methods	370:394	optimal treatment methods for hyaluronic acid-related vascular occlusion	370:441	Our study was designed to explore optimal treatment methods for hyaluronic acid-related vascular occlusion.					
30921126	0	34	theme	Acid	55:58	arg1	Injection					60:68	Hyaluronic Acid Injection	44:68	Hyaluronic Acid Injection	44:68	Experimentally Induced Arterial Embolism by Hyaluronic Acid Injection: Clinicopathologic Observations and Treatment.					
30921126	3	35	theme	postinjection	653:665	arg1	points					672:677	different postinjection time points	643:677	different postinjection time points	643:677	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	8	36	theme	group	1403:1407	arg1	B					1409:1409	43.29 ± 9.28 percent; group B	1381:1409	43.29 ± 9.28 percent; group B	1381:1409	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	3	37	theme	intraarterial	503:515	arg1	acid					528:531	intraarterial hyaluronic acid	503:531	intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model	503:617	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	8	38	theme	71.92	1507:1511	arg1	percent					1521:1527	71.92 ± 19.06 percent	1507:1527	71.92 ± 19.06 percent	1507:1527	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	38	theme	71.92	1507:1511	arg1	E					1504:1504	group E	1498:1504	group E	1498:1504	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	3	39	theme	bilateral	551:559	arg1	inferior					561:568	the bilateral inferior epigastric arteries to establish an animal model	547:617	the bilateral inferior epigastric arteries to establish an animal model	547:617	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	8	40	theme	±	1549:1549	arg1	A					1378:1378	group A	1372:1378	group A	1372:1378	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	40	theme	±	1549:1549	arg1	percent					1557:1563	57.47 ± 13.64 percent	1543:1563	57.47 ± 13.64 percent	1543:1563	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	5	41	theme	epigastric	837:846	arg1	artery					848:853	the inferior epigastric artery	824:853	the inferior epigastric artery	824:853	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	1	42	theme	applications	276:287	arg1	number					225:230	the rapidly growing number	205:230	the rapidly growing number of procedures performed and their expanding applications	205:287	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	10	43	dep	groups	1684:1689	arg1	groups					1684:1689	groups A and F	1684:1697	groups A and F (p > 0.05)	1684:1708	No significant difference was observed between groups A and F (p > 0.05).					
30921126	10	43	dep	groups	1684:1689	arg1	F					1697:1697	F	1697:1697	F	1697:1697	No significant difference was observed between groups A and F (p > 0.05).					
30921126	10	43	dep	groups	1684:1689	arg1	A					1691:1691	A	1691:1691	A	1691:1691	No significant difference was observed between groups A and F (p > 0.05).					
30921126	11	44	theme	hyaluronic	1830:1839	arg1	acid					1841:1844	hyaluronic acid	1830:1844	hyaluronic acid	1830:1844	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	1	45	theme	such	290:293	arg1	complications					295:307	such complications	290:307	such complications	290:307	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	8	46	theme	group	1534:1538	arg1	F					1540:1540	group F	1534:1540	group F	1534:1540	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	3	47	theme	epigastric	570:579	arg1	arteries					581:588	epigastric arteries	570:588	epigastric arteries	570:588	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	7	48	theme	pathologic	1090:1099	arg1	examination					1101:1111	pathologic examination	1090:1111	pathologic examination	1090:1111	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	6	49	theme	flap	1002:1005	arg1	rate					1016:1019	The flap survival rate	998:1019	The flap survival rate	998:1019	The flap survival rate was calculated and analyzed.					
30921126	7	50	theme	study	1083:1087	arg1	part					1071:1074	the first part	1061:1074	the first part of the study	1061:1087	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	11	51	theme	injection-related	1846:1862	arg1	embolism					1873:1880	injection-related arterial embolism	1846:1880	injection-related arterial embolism	1846:1880	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	1	52	theme	growing	217:223	arg1	number					225:230	the rapidly growing number	205:230	the rapidly growing number of procedures performed and their expanding applications	205:287	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	8	53	theme	±	1418:1418	arg1	percent					1426:1432	54.17 ± 10.86 percent	1412:1432	54.17 ± 10.86 percent	1412:1432	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	54	theme	second	1290:1295	arg1	part					1297:1300	the second part	1286:1300	the second part of the study	1286:1313	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	10	55	theme	significant	1640:1650	arg1	difference					1652:1661	No significant difference	1637:1661	No significant difference	1637:1661	No significant difference was observed between groups A and F (p > 0.05).					
30921126	5	56	theme	rats	886:889	arg1	rats					886:889	rats	886:889	rats	886:889	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	5	56	theme	rats	886:889	arg1	groups					876:881	six groups	872:881	six groups of rats	872:889	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	4	57	theme	inferior	684:691	arg1	artery					704:709	The inferior epigastric artery	680:709	The inferior epigastric artery	680:709	The inferior epigastric artery was retrieved for pathologic examination.					
30921126	7	58	theme	first	1065:1069	arg1	part					1071:1074	the first part	1061:1074	the first part of the study	1061:1087	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	1	59	theme	major	137:141	arg1	complications					143:155	major complications	137:155	major complications of hyaluronic acid injection	137:184	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	8	60	theme	group	1435:1439	arg1	percent					1458:1464	59.27 ± 13.40 percent	1444:1464	59.27 ± 13.40 percent	1444:1464	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	60	theme	group	1435:1439	arg1	C					1441:1441	group C	1435:1441	group C	1435:1441	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	61	theme	study	1309:1313	arg1	part					1297:1300	the second part	1286:1300	the second part of the study	1286:1313	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	3	62	theme	time	667:670	arg1	points					672:677	different postinjection time points	643:677	different postinjection time points	643:677	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	8	63	theme	flap	1316:1319	arg1	rates					1330:1334	flap survival rates	1316:1334	flap survival rates (mean percentages)	1316:1353	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	63	theme	flap	1316:1319	arg1	percentages					1342:1352	mean percentages	1337:1352	mean percentages	1337:1352	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	5	64	theme	abdominal	796:804	arg1	flaps					806:810	bilateral abdominal flaps	786:810	bilateral abdominal flaps supplied by the inferior epigastric artery	786:853	In the second part of the study, bilateral abdominal flaps supplied by the inferior epigastric artery were elevated in six groups of rats, and hyaluronic acid was injected into the right side, with each group receiving a different intervention.					
30921126	3	65	theme	different	643:651	arg1	points					672:677	different postinjection time points	643:677	different postinjection time points	643:677	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	1	66	theme	acid	171:174	arg1	injection					176:184	hyaluronic acid injection	160:184	hyaluronic acid injection	160:184	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	8	67	theme	±	1450:1450	arg1	percent					1458:1464	59.27 ± 13.40 percent	1444:1464	59.27 ± 13.40 percent	1444:1464	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	67	theme	±	1450:1450	arg1	C					1441:1441	group C	1435:1441	group C	1435:1441	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	4	68	theme	pathologic	729:738	arg1	examination					740:750	pathologic examination	729:750	pathologic examination	729:750	The inferior epigastric artery was retrieved for pathologic examination.					
30921126	9	69	dep	differed	1574:1581	arg1	<					1627:1627	p < 0.001	1625:1633	p < 0.001	1625:1633	Group A differed significantly from groups B, C, D, and E (p < 0.001).					
30921126	11	70	dep	embolism	1873:1880	arg1	minutes					1891:1897	45 minutes	1888:1897	45 minutes	1888:1897	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	11	70	dep	embolism	1873:1880	arg1	hours					1906:1910	24 hours	1903:1910	24 hours	1903:1910	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	8	71	theme	mean	1337:1340	arg1	rates					1330:1334	flap survival rates	1316:1334	flap survival rates (mean percentages)	1316:1353	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	71	theme	mean	1337:1340	arg1	percentages					1342:1352	mean percentages	1337:1352	mean percentages	1337:1352	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	7	72	theme	hyaluronic	1228:1237	arg1	acid					1239:1242	pure hyaluronic acid	1223:1242	pure hyaluronic acid	1223:1242	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	11	73	theme	arterial	1864:1871	arg1	embolism					1873:1880	injection-related arterial embolism	1846:1880	injection-related arterial embolism	1846:1880	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	9	74	theme	p	1625:1625	arg1	<					1627:1627	p < 0.001	1625:1633	p < 0.001	1625:1633	Group A differed significantly from groups B, C, D, and E (p < 0.001).					
30921126	8	75	theme	group	1467:1471	arg1	percent					1489:1495	64.37 ± 8.61 percent	1476:1495	64.37 ± 8.61 percent	1476:1495	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	75	theme	group	1467:1471	arg1	D					1473:1473	group D	1467:1473	group D	1467:1473	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	0	76	theme	Induced	15:21	arg1	Embolism					32:39	Experimentally Induced Arterial Embolism	0:39	Experimentally Induced Arterial Embolism by Hyaluronic Acid Injection: Clinicopathologic Observations and Treatment.	0:115	Experimentally Induced Arterial Embolism by Hyaluronic Acid Injection: Clinicopathologic Observations and Treatment.					
30921126	3	77	from	METHODS	444:450	arg1	part					465:468	the first part	455:468	the first part of the study	455:481	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	8	78	theme	±	1482:1482	arg1	percent					1489:1495	64.37 ± 8.61 percent	1476:1495	64.37 ± 8.61 percent	1476:1495	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	78	theme	±	1482:1482	arg1	D					1473:1473	group D	1467:1473	group D	1467:1473	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	2	79	theme	vascular	424:431	arg1	occlusion					433:441	hyaluronic acid-related vascular occlusion	400:441	hyaluronic acid-related vascular occlusion	400:441	Our study was designed to explore optimal treatment methods for hyaluronic acid-related vascular occlusion.					
30921126	7	80	theme	hyaluronic	1176:1185	arg1	occlusion					1200:1208	arterial hyaluronic acid-induced occlusion	1167:1208	arterial hyaluronic acid-induced occlusion	1167:1208	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	1	81	theme	greater	317:323	arg1	attention					325:333	greater attention	317:333	greater attention	317:333	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
30921126	11	82	theme	hyaluronidase	1771:1783	arg1	use					1736:1738	The combined use	1723:1738	The combined use of intravenous or subcutaneous hyaluronidase	1723:1783	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	2	83	theme	acid-related	411:422	arg1	occlusion					433:441	hyaluronic acid-related vascular occlusion	400:441	hyaluronic acid-related vascular occlusion	400:441	Our study was designed to explore optimal treatment methods for hyaluronic acid-related vascular occlusion.					
30921126	0	84	theme	Hyaluronic	44:53	arg1	Injection					60:68	Hyaluronic Acid Injection	44:68	Hyaluronic Acid Injection	44:68	Experimentally Induced Arterial Embolism by Hyaluronic Acid Injection: Clinicopathologic Observations and Treatment.					
30921126	8	85	dep	were	1355:1358	arg1	percent					1521:1527	71.92 ± 19.06 percent	1507:1527	71.92 ± 19.06 percent	1507:1527	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	D					1473:1473	group D	1467:1473	group D	1467:1473	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	E					1504:1504	group E	1498:1504	group E	1498:1504	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	percent					1426:1432	54.17 ± 10.86 percent	1412:1432	54.17 ± 10.86 percent	1412:1432	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	A					1378:1378	group A	1372:1378	group A	1372:1378	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	percent					1458:1464	59.27 ± 13.40 percent	1444:1464	59.27 ± 13.40 percent	1444:1464	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	B					1409:1409	43.29 ± 9.28 percent; group B	1381:1409	43.29 ± 9.28 percent; group B	1381:1409	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	percent					1557:1563	57.47 ± 13.64 percent	1543:1563	57.47 ± 13.64 percent	1543:1563	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	F					1540:1540	group F	1534:1540	group F	1534:1540	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	percent					1489:1495	64.37 ± 8.61 percent	1476:1495	64.37 ± 8.61 percent	1476:1495	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	85	dep	were	1355:1358	arg1	C					1441:1441	group C	1435:1441	group C	1435:1441	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	86	theme	group	1498:1502	arg1	percent					1521:1527	71.92 ± 19.06 percent	1507:1527	71.92 ± 19.06 percent	1507:1527	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	86	theme	group	1498:1502	arg1	E					1504:1504	group E	1498:1504	group E	1498:1504	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	7	87	theme	pure	1223:1226	arg1	acid					1239:1242	pure hyaluronic acid	1223:1242	pure hyaluronic acid	1223:1242	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	7	88	theme	emboli	1150:1155	arg1	composition					1131:1141	the composition	1127:1141	the composition of the emboli caused by arterial hyaluronic acid-induced occlusion	1127:1208	RESULTS In the first part of the study, pathologic examination revealed that the composition of the emboli caused by arterial hyaluronic acid-induced occlusion changed from pure hyaluronic acid to a hyaluronic acid-thrombus mixture.					
30921126	11	89	dep	CONCLUSIONS	1711:1721	arg1	use					1736:1738	The combined use	1723:1738	The combined use of intravenous or subcutaneous hyaluronidase	1723:1783	CONCLUSIONS The combined use of intravenous or subcutaneous hyaluronidase and urokinase was most effective in treating hyaluronic acid injection-related arterial embolism after 45 minutes and 24 hours.					
30921126	2	90	theme	optimal	370:376	arg1	methods					388:394	optimal treatment methods	370:394	optimal treatment methods for hyaluronic acid-related vascular occlusion	370:441	Our study was designed to explore optimal treatment methods for hyaluronic acid-related vascular occlusion.					
30921126	8	91	theme	57.47	1543:1547	arg1	A					1378:1378	group A	1372:1378	group A	1372:1378	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	91	theme	57.47	1543:1547	arg1	percent					1557:1563	57.47 ± 13.64 percent	1543:1563	57.47 ± 13.64 percent	1543:1563	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	0	92	dep	Embolism	32:39	arg1	Observations					89:100	Clinicopathologic Observations	71:100	Clinicopathologic Observations	71:100	Experimentally Induced Arterial Embolism by Hyaluronic Acid Injection: Clinicopathologic Observations and Treatment.					
30921126	0	92	dep	Embolism	32:39	arg1	Treatment					106:114	Treatment	106:114	Treatment	106:114	Experimentally Induced Arterial Embolism by Hyaluronic Acid Injection: Clinicopathologic Observations and Treatment.					
30921126	8	93	theme	±	1513:1513	arg1	percent					1521:1527	71.92 ± 19.06 percent	1507:1527	71.92 ± 19.06 percent	1507:1527	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	8	93	theme	±	1513:1513	arg1	E					1504:1504	group E	1498:1504	group E	1498:1504	In the second part of the study, flap survival rates (mean percentages) were as follows: group A, 43.29 ± 9.28 percent; group B, 54.17 ± 10.86 percent; group C, 59.27 ± 13.40 percent; group D, 64.37 ± 8.61 percent; group E, 71.92 ± 19.06 percent; and group F, 57.47 ± 13.64 percent.					
30921126	10	94	theme	p	1700:1700	arg1	groups					1684:1689	groups A and F	1684:1697	groups A and F (p > 0.05)	1684:1708	No significant difference was observed between groups A and F (p > 0.05).					
30921126	10	94	theme	p	1700:1700	arg1	>					1702:1702	p > 0.05	1700:1707	p > 0.05	1700:1707	No significant difference was observed between groups A and F (p > 0.05).					
30921126	3	95	theme	hyaluronic	517:526	arg1	acid					528:531	intraarterial hyaluronic acid	503:531	intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model	503:617	METHODS In the first part of the study, 60 rats were given intraarterial hyaluronic acid injected into the bilateral inferior epigastric arteries to establish an animal model, and were euthanized at different postinjection time points.					
30921126	2	96	theme	hyaluronic	400:409	arg1	occlusion					433:441	hyaluronic acid-related vascular occlusion	400:441	hyaluronic acid-related vascular occlusion	400:441	Our study was designed to explore optimal treatment methods for hyaluronic acid-related vascular occlusion.					
30921126	1	97	theme	expanding	266:274	arg1	applications					276:287	their expanding applications	260:287	their expanding applications	260:287	BACKGROUND Although major complications of hyaluronic acid injection rarely occur, with the rapidly growing number of procedures performed and their expanding applications, such complications warrant greater attention.					
29670183	2	0	theme	olytic	449:454	arg1	activity					456:463	fibrin(ogen)olytic activity	437:463	fibrin(ogen)olytic activity	437:463	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	1	1	theme	therapeutic	296:306	arg1	agents					308:313	novel, potent and safe therapeutic agents	273:313	novel, potent and safe therapeutic agents	273:313	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	4	2	theme	FXa	842:844	arg1	inhibition					815:824	its dual inhibition	806:824	its dual inhibition of thrombin and FXa by a non-enzymatic mechanism	806:873	Lunathrombase is an αβ-fibrinogenase, demonstrating anticoagulant activity with its dual inhibition of thrombin and FXa by a non-enzymatic mechanism.					
29670183	9	3	from	effects	1645:1651	arg1	animals					1664:1670	treated animals	1656:1670	treated animals	1656:1670	Lunathrombase (10 mg/kg) did not show toxicity or adverse pharmacological effects in treated animals.					
29670183	1	4	theme	bioactive	224:232	arg1	molecules					234:242	bioactive molecules	224:242	bioactive molecules	224:242	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	0	5	theme	safety	81:86	arg1	mechanism					14:22	Anticoagulant mechanism	0:22	Anticoagulant mechanism	0:22	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	0	5	theme	safety	81:86	arg1	activity					41:48	pharmacological activity	25:48	pharmacological activity	25:48	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	0	5	theme	safety	81:86	arg1	assessment					55:64	assessment	55:64	assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica	55:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	1	6	theme	agents	308:313	arg1	discovery					260:268	the discovery	256:268	the discovery of novel, potent and safe therapeutic agents	256:313	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	0	7	theme	Leucas	149:154	arg1	indica					156:161	Leucas indica	149:161	Leucas indica	149:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	5	8	theme	Spectrofluorometric	876:894	arg1	analyses					924:931	Spectrofluorometric and isothermal calorimetric analyses	876:931	Spectrofluorometric and isothermal calorimetric analyses	876:931	Spectrofluorometric and isothermal calorimetric analyses revealed the binding of lunathrombase to fibrinogen, thrombin, and/or FXa with the generation of endothermic heat.					
29670183	0	9	from	leaves	139:144	arg1	safety					81:86	preclinical safety	69:86	preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica	69:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	0	9	from	leaves	139:144	arg1	protease					125:132	a novel fibrin(ogen)olytic serine protease	91:132	a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica	91:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	0	10	theme	novel	93:97	arg1	ogen					106:109	ogen	106:109	ogen	106:109	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	0	10	theme	novel	93:97	arg1	fibrin					99:104	a novel fibrin	91:104	a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica	91:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	8	11	theme	in	1490:1491	arg1	anticoagulant					1498:1510	in vivo anticoagulant	1490:1510	in vivo anticoagulant	1490:1510	Lunathrombase was non-cytotoxic to mammalian cells, non-hemolytic, and demonstrated dose-dependent (0.125-0.5 mg/kg) in vivo anticoagulant and plasma defibrinogenation activities in a rodent model.					
29670183	2	12	theme	aqueous	545:551	arg1	extract					534:540	an extract	531:540	an extract of aqueous leaves	531:558	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	9	13	from	toxicity	1609:1616	arg1	animals					1664:1670	treated animals	1656:1670	treated animals	1656:1670	Lunathrombase (10 mg/kg) did not show toxicity or adverse pharmacological effects in treated animals.					
29670183	5	14	theme	lunathrombase	957:969	arg1	binding					946:952	the binding	942:952	the binding of lunathrombase to fibrinogen, thrombin, and/or FXa	942:1005	Spectrofluorometric and isothermal calorimetric analyses revealed the binding of lunathrombase to fibrinogen, thrombin, and/or FXa with the generation of endothermic heat.					
29670183	2	15	theme	N-	479:480	arg1	devoid					469:474	devoid	469:474	devoid	469:474	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	9	16	theme	adverse	1621:1627	arg1	effects					1645:1651	adverse pharmacological effects	1621:1651	adverse pharmacological effects	1621:1651	Lunathrombase (10 mg/kg) did not show toxicity or adverse pharmacological effects in treated animals.					
29670183	2	17	link	linked	489:494	arg1	oligosaccharides					496:511	O- linked oligosaccharides	486:511	O- linked oligosaccharides	486:511	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	8	18	dep	in	1490:1491	arg1	vivo					1493:1496	vivo	1493:1496	vivo	1493:1496	Lunathrombase was non-cytotoxic to mammalian cells, non-hemolytic, and demonstrated dose-dependent (0.125-0.5 mg/kg) in vivo anticoagulant and plasma defibrinogenation activities in a rodent model.					
29670183	1	19	theme	thrombosis-associated	324:344	arg1	diseases					361:368	thrombosis-associated cardiovascular diseases	324:368	thrombosis-associated cardiovascular diseases	324:368	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	9	20	theme	pharmacological	1629:1643	arg1	effects					1645:1651	adverse pharmacological effects	1621:1651	adverse pharmacological effects	1621:1651	Lunathrombase (10 mg/kg) did not show toxicity or adverse pharmacological effects in treated animals.					
29670183	4	21	theme	dual	810:813	arg1	inhibition					815:824	its dual inhibition	806:824	its dual inhibition of thrombin and FXa by a non-enzymatic mechanism	806:873	Lunathrombase is an αβ-fibrinogenase, demonstrating anticoagulant activity with its dual inhibition of thrombin and FXa by a non-enzymatic mechanism.					
29670183	0	22	theme	fibrin	99:104	arg1	protease					125:132	a novel fibrin(ogen)olytic serine protease	91:132	a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica	91:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	1	23	theme	cardiovascular	346:359	arg1	diseases					361:368	thrombosis-associated cardiovascular diseases	324:368	thrombosis-associated cardiovascular diseases	324:368	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	2	24	dep	protease	404:411	arg1	showing					429:435	showing	429:435	showing fibrin(ogen)olytic activity	429:463	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	2	24	dep	protease	404:411	arg1	devoid					469:474	devoid	469:474	devoid	469:474	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	6	25	theme	platelet	1109:1116	arg1	aggregation					1118:1128	collagen/ADP/arachidonic acid-induced mammalian platelet aggregation	1061:1128	collagen/ADP/arachidonic acid-induced mammalian platelet aggregation	1061:1128	It inhibited collagen/ADP/arachidonic acid-induced mammalian platelet aggregation, and demonstrated antiplatelet activity via COX-1 inhibition and the upregulation of the cAMP level.					
29670183	2	26	theme	35 kDa	373:378	arg1	lunathrombase					414:426	lunathrombase	414:426	lunathrombase	414:426	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	2	26	theme	35 kDa	373:378	arg1	protease					404:411	A 35 kDa (m/z 34747.5230) serine protease	371:411	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides	371:511	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	3	27	theme	composition	655:665	arg1	determination					667:679	amino acid composition determination	644:679	amino acid composition determination	644:679	The LC-MS/MS analysis, de novo sequencing, secondary structure, and amino acid composition determination suggested the enzyme's novel characteristic.					
29670183	4	28	theme	thrombin	829:836	arg1	inhibition					815:824	its dual inhibition	806:824	its dual inhibition of thrombin and FXa by a non-enzymatic mechanism	806:873	Lunathrombase is an αβ-fibrinogenase, demonstrating anticoagulant activity with its dual inhibition of thrombin and FXa by a non-enzymatic mechanism.					
29670183	7	29	theme	thrombolytic	1261:1272	arg1	activity					1274:1281	in vitro thrombolytic activity	1252:1281	in vitro thrombolytic activity	1252:1281	Lunathrombase showed in vitro thrombolytic activity and was not inhibited by endogenous protease inhibitors α2 macroglobulin and antiplasmin.					
29670183	4	30	theme	non-enzymatic	851:863	arg1	mechanism					865:873	a non-enzymatic mechanism	849:873	a non-enzymatic mechanism	849:873	Lunathrombase is an αβ-fibrinogenase, demonstrating anticoagulant activity with its dual inhibition of thrombin and FXa by a non-enzymatic mechanism.					
29670183	6	31	theme	collagen/ADP/arachidonic	1061:1084	arg1	aggregation					1118:1128	collagen/ADP/arachidonic acid-induced mammalian platelet aggregation	1061:1128	collagen/ADP/arachidonic acid-induced mammalian platelet aggregation	1061:1128	It inhibited collagen/ADP/arachidonic acid-induced mammalian platelet aggregation, and demonstrated antiplatelet activity via COX-1 inhibition and the upregulation of the cAMP level.					
29670183	0	32	theme	Anticoagulant	0:12	arg1	mechanism					14:22	Anticoagulant mechanism	0:22	Anticoagulant mechanism	0:22	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	2	33	theme	L.	565:566	arg1	indica					568:573	L. indica	565:573	L. indica	565:573	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	8	34	theme	rodent	1557:1562	arg1	model					1564:1568	a rodent model	1555:1568	a rodent model	1555:1568	Lunathrombase was non-cytotoxic to mammalian cells, non-hemolytic, and demonstrated dose-dependent (0.125-0.5 mg/kg) in vivo anticoagulant and plasma defibrinogenation activities in a rodent model.					
29670183	3	35	theme	de	599:600	arg1	sequencing					607:616	de novo sequencing	599:616	de novo sequencing	599:616	The LC-MS/MS analysis, de novo sequencing, secondary structure, and amino acid composition determination suggested the enzyme's novel characteristic.					
29670183	0	36	theme	pharmacological	25:39	arg1	activity					41:48	pharmacological activity	25:48	pharmacological activity	25:48	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	3	37	theme	novel	704:708	arg1	characteristic					710:723	the enzyme's novel characteristic	691:723	the enzyme's novel characteristic	691:723	The LC-MS/MS analysis, de novo sequencing, secondary structure, and amino acid composition determination suggested the enzyme's novel characteristic.					
29670183	7	38	dep	in	1252:1253	arg1	vitro					1255:1259	vitro	1255:1259	vitro	1255:1259	Lunathrombase showed in vitro thrombolytic activity and was not inhibited by endogenous protease inhibitors α2 macroglobulin and antiplasmin.					
29670183	1	39	theme	molecules	234:242	arg1	plethora					212:219	a plethora	210:219	a plethora of bioactive molecules	210:242	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	2	40	theme	serine	397:402	arg1	lunathrombase					414:426	lunathrombase	414:426	lunathrombase	414:426	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	2	40	theme	serine	397:402	arg1	protease					404:411	A 35 kDa (m/z 34747.5230) serine protease	371:411	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides	371:511	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	9	41	theme	treated	1656:1662	arg1	animals					1664:1670	treated animals	1656:1670	treated animals	1656:1670	Lunathrombase (10 mg/kg) did not show toxicity or adverse pharmacological effects in treated animals.					
29670183	2	42	theme	oligosaccharides	496:511	arg1	devoid					469:474	devoid	469:474	devoid	469:474	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	8	43	theme	defibrinogenation	1523:1539	arg1	activities					1541:1550	plasma defibrinogenation activities	1516:1550	plasma defibrinogenation activities	1516:1550	Lunathrombase was non-cytotoxic to mammalian cells, non-hemolytic, and demonstrated dose-dependent (0.125-0.5 mg/kg) in vivo anticoagulant and plasma defibrinogenation activities in a rodent model.					
29670183	6	44	theme	COX-1	1174:1178	arg1	inhibition					1180:1189	COX-1 inhibition	1174:1189	COX-1 inhibition	1174:1189	It inhibited collagen/ADP/arachidonic acid-induced mammalian platelet aggregation, and demonstrated antiplatelet activity via COX-1 inhibition and the upregulation of the cAMP level.					
29670183	2	45	dep	aqueous	545:551	arg1	leaves					553:558	leaves	553:558	leaves	553:558	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	0	46	theme	serine	118:123	arg1	protease					125:132	a novel fibrin(ogen)olytic serine protease	91:132	a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica	91:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	0	47	from	safety	81:86	arg1	leaves					139:144	leaves	139:144	leaves	139:144	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	8	48	dep	non-cytotoxic	1391:1403	arg1	non-hemolytic					1425:1437	non-hemolytic	1425:1437	non-hemolytic	1425:1437	Lunathrombase was non-cytotoxic to mammalian cells, non-hemolytic, and demonstrated dose-dependent (0.125-0.5 mg/kg) in vivo anticoagulant and plasma defibrinogenation activities in a rodent model.					
29670183	1	49	theme	medicinal	182:190	arg1	plants					192:197	medicinal plants	182:197	medicinal plants containing a plethora of bioactive molecules	182:242	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	6	50	theme	mammalian	1099:1107	arg1	aggregation					1118:1128	collagen/ADP/arachidonic acid-induced mammalian platelet aggregation	1061:1128	collagen/ADP/arachidonic acid-induced mammalian platelet aggregation	1061:1128	It inhibited collagen/ADP/arachidonic acid-induced mammalian platelet aggregation, and demonstrated antiplatelet activity via COX-1 inhibition and the upregulation of the cAMP level.					
29670183	1	51	theme	novel	273:277	arg1	agents					308:313	novel, potent and safe therapeutic agents	273:313	novel, potent and safe therapeutic agents	273:313	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	0	52	theme	olytic	111:116	arg1	protease					125:132	a novel fibrin(ogen)olytic serine protease	91:132	a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica	91:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	3	53	theme	LC-MS/MS	580:587	arg1	analysis					589:596	The LC-MS/MS analysis	576:596	The LC-MS/MS analysis	576:596	The LC-MS/MS analysis, de novo sequencing, secondary structure, and amino acid composition determination suggested the enzyme's novel characteristic.					
29670183	4	54	theme	anticoagulant	778:790	arg1	activity					792:799	anticoagulant activity	778:799	anticoagulant activity	778:799	Lunathrombase is an αβ-fibrinogenase, demonstrating anticoagulant activity with its dual inhibition of thrombin and FXa by a non-enzymatic mechanism.					
29670183	2	55	theme	fibrin	437:442	arg1	activity					456:463	fibrin(ogen)olytic activity	437:463	fibrin(ogen)olytic activity	437:463	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	2	56	dep	linked	489:494	arg1	O-					486:487	O-	486:487	O-	486:487	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	8	57	theme	mammalian	1408:1416	arg1	cells					1418:1422	mammalian cells	1408:1422	mammalian cells	1408:1422	Lunathrombase was non-cytotoxic to mammalian cells, non-hemolytic, and demonstrated dose-dependent (0.125-0.5 mg/kg) in vivo anticoagulant and plasma defibrinogenation activities in a rodent model.					
29670183	5	58	theme	heat	1042:1045	arg1	generation					1016:1025	the generation	1012:1025	the generation of endothermic heat	1012:1045	Spectrofluorometric and isothermal calorimetric analyses revealed the binding of lunathrombase to fibrinogen, thrombin, and/or FXa with the generation of endothermic heat.					
29670183	3	59	theme	acid	650:653	arg1	determination					667:679	amino acid composition determination	644:679	amino acid composition determination	644:679	The LC-MS/MS analysis, de novo sequencing, secondary structure, and amino acid composition determination suggested the enzyme's novel characteristic.					
29670183	7	60	theme	protease	1319:1326	arg1	inhibitors					1328:1337	endogenous protease inhibitors	1308:1337	endogenous protease inhibitors α2 macroglobulin and antiplasmin	1308:1370	Lunathrombase showed in vitro thrombolytic activity and was not inhibited by endogenous protease inhibitors α2 macroglobulin and antiplasmin.					
29670183	1	61	theme	potent	280:285	arg1	agents					308:313	novel, potent and safe therapeutic agents	273:313	novel, potent and safe therapeutic agents	273:313	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	0	62	theme	protease	125:132	arg1	safety					81:86	preclinical safety	69:86	preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica	69:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	5	63	theme	endothermic	1030:1040	arg1	heat					1042:1045	endothermic heat	1030:1045	endothermic heat	1030:1045	Spectrofluorometric and isothermal calorimetric analyses revealed the binding of lunathrombase to fibrinogen, thrombin, and/or FXa with the generation of endothermic heat.					
29670183	1	64	contain	containing	199:208	arg2	plethora					212:219	a plethora	210:219	a plethora of bioactive molecules	210:242	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	1	64	contain	containing	199:208	arg1	plants					192:197	medicinal plants	182:197	medicinal plants containing a plethora of bioactive molecules	182:242	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	5	65	theme	isothermal	900:909	arg1	analyses					924:931	Spectrofluorometric and isothermal calorimetric analyses	876:931	Spectrofluorometric and isothermal calorimetric analyses	876:931	Spectrofluorometric and isothermal calorimetric analyses revealed the binding of lunathrombase to fibrinogen, thrombin, and/or FXa with the generation of endothermic heat.					
29670183	8	66	theme	plasma	1516:1521	arg1	activities					1541:1550	plasma defibrinogenation activities	1516:1550	plasma defibrinogenation activities	1516:1550	Lunathrombase was non-cytotoxic to mammalian cells, non-hemolytic, and demonstrated dose-dependent (0.125-0.5 mg/kg) in vivo anticoagulant and plasma defibrinogenation activities in a rodent model.					
29670183	6	67	theme	level	1224:1228	arg1	activity					1161:1168	antiplatelet activity	1148:1168	antiplatelet activity via COX-1 inhibition	1148:1189	It inhibited collagen/ADP/arachidonic acid-induced mammalian platelet aggregation, and demonstrated antiplatelet activity via COX-1 inhibition and the upregulation of the cAMP level.					
29670183	6	67	theme	level	1224:1228	arg1	upregulation					1199:1210	the upregulation	1195:1210	the upregulation of the cAMP level	1195:1228	It inhibited collagen/ADP/arachidonic acid-induced mammalian platelet aggregation, and demonstrated antiplatelet activity via COX-1 inhibition and the upregulation of the cAMP level.					
29670183	3	68	theme	secondary	619:627	arg1	structure					629:637	secondary structure	619:637	secondary structure	619:637	The LC-MS/MS analysis, de novo sequencing, secondary structure, and amino acid composition determination suggested the enzyme's novel characteristic.					
29670183	8	69	theme	dose-dependent	1457:1470	arg1	anticoagulant					1498:1510	in vivo anticoagulant	1490:1510	in vivo anticoagulant	1490:1510	Lunathrombase was non-cytotoxic to mammalian cells, non-hemolytic, and demonstrated dose-dependent (0.125-0.5 mg/kg) in vivo anticoagulant and plasma defibrinogenation activities in a rodent model.					
29670183	5	70	theme	calorimetric	911:922	arg1	analyses					924:931	Spectrofluorometric and isothermal calorimetric analyses	876:931	Spectrofluorometric and isothermal calorimetric analyses	876:931	Spectrofluorometric and isothermal calorimetric analyses revealed the binding of lunathrombase to fibrinogen, thrombin, and/or FXa with the generation of endothermic heat.					
29670183	7	71	theme	in	1252:1253	arg1	activity					1274:1281	in vitro thrombolytic activity	1252:1281	in vitro thrombolytic activity	1252:1281	Lunathrombase showed in vitro thrombolytic activity and was not inhibited by endogenous protease inhibitors α2 macroglobulin and antiplasmin.					
29670183	6	72	theme	cAMP	1219:1222	arg1	level					1224:1228	the cAMP level	1215:1228	the cAMP level	1215:1228	It inhibited collagen/ADP/arachidonic acid-induced mammalian platelet aggregation, and demonstrated antiplatelet activity via COX-1 inhibition and the upregulation of the cAMP level.					
29670183	7	73	theme	endogenous	1308:1317	arg1	inhibitors					1328:1337	endogenous protease inhibitors	1308:1337	endogenous protease inhibitors α2 macroglobulin and antiplasmin	1308:1370	Lunathrombase showed in vitro thrombolytic activity and was not inhibited by endogenous protease inhibitors α2 macroglobulin and antiplasmin.					
29670183	6	74	theme	antiplatelet	1148:1159	arg1	activity					1161:1168	antiplatelet activity	1148:1168	antiplatelet activity via COX-1 inhibition	1148:1189	It inhibited collagen/ADP/arachidonic acid-induced mammalian platelet aggregation, and demonstrated antiplatelet activity via COX-1 inhibition and the upregulation of the cAMP level.					
29670183	0	75	theme	preclinical	69:79	arg1	safety					81:86	preclinical safety	69:86	preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica	69:161	Anticoagulant mechanism, pharmacological activity, and assessment of preclinical safety of a novel fibrin(ogen)olytic serine protease from leaves of Leucas indica.					
29670183	3	76	dep	de	599:600	arg1	novo					602:605	novo	602:605	novo	602:605	The LC-MS/MS analysis, de novo sequencing, secondary structure, and amino acid composition determination suggested the enzyme's novel characteristic.					
29670183	6	77	theme	acid-induced	1086:1097	arg1	aggregation					1118:1128	collagen/ADP/arachidonic acid-induced mammalian platelet aggregation	1061:1128	collagen/ADP/arachidonic acid-induced mammalian platelet aggregation	1061:1128	It inhibited collagen/ADP/arachidonic acid-induced mammalian platelet aggregation, and demonstrated antiplatelet activity via COX-1 inhibition and the upregulation of the cAMP level.					
29670183	1	78	theme	safe	291:294	arg1	agents					308:313	novel, potent and safe therapeutic agents	273:313	novel, potent and safe therapeutic agents	273:313	The harnessing of medicinal plants containing a plethora of bioactive molecules may lead to the discovery of novel, potent and safe therapeutic agents to treat thrombosis-associated cardiovascular diseases.					
29670183	2	79	dep	35 kDa	373:378	arg1	m/z					381:383	m/z 34747.5230	381:394	m/z 34747.5230	381:394	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29670183	3	80	theme	amino	644:648	arg1	determination					667:679	amino acid composition determination	644:679	amino acid composition determination	644:679	The LC-MS/MS analysis, de novo sequencing, secondary structure, and amino acid composition determination suggested the enzyme's novel characteristic.					
29670183	8	81	dep	dose-dependent	1457:1470	arg1	0.125-0.5 mg/kg					1473:1487	0.125-0.5 mg/kg	1473:1487	0.125-0.5 mg/kg	1473:1487	Lunathrombase was non-cytotoxic to mammalian cells, non-hemolytic, and demonstrated dose-dependent (0.125-0.5 mg/kg) in vivo anticoagulant and plasma defibrinogenation activities in a rodent model.					
29670183	2	82	theme	linked	489:494	arg1	oligosaccharides					496:511	O- linked oligosaccharides	486:511	O- linked oligosaccharides	486:511	A 35 kDa (m/z 34747.5230) serine protease (lunathrombase) showing fibrin(ogen)olytic activity and devoid of N- and O- linked oligosaccharides was purified from an extract of aqueous leaves from L. indica.					
29859279	11	0	theme	enzyme	1325:1330	arg1	films					1340:1344	the Pulpzyme HC 2500 enzyme treated films	1304:1344	the Pulpzyme HC 2500 enzyme treated films	1304:1344	Higher thermal stability, thermo-mechanical and mechanical properties were determined in the Pulpzyme HC 2500 enzyme treated films.					
29859279	1	1	theme	treated	302:308	arg1	pulps					322:326	enzyme treated kraft-NaBH4 pulps	295:326	enzyme treated kraft-NaBH4 pulps	295:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	11	2	theme	thermal	1222:1228	arg1	stability					1230:1238	Higher thermal stability	1215:1238	Higher thermal stability	1215:1238	Higher thermal stability, thermo-mechanical and mechanical properties were determined in the Pulpzyme HC 2500 enzyme treated films.					
29859279	2	3	theme	Morphological	329:341	arg1	alterations					343:353	Morphological alterations	329:353	Morphological alterations	329:353	Morphological alterations were monitored with SEM.					
29859279	10	4	theme	CNF	1204:1206	arg1	films					1208:1212	the neat CNF films	1195:1212	the neat CNF films	1195:1212	The PCNF and SiCNF films exhibit more visible dielectric behavior than that of the neat CNF films.					
29859279	10	5	theme	dielectric	1162:1171	arg1	behavior					1173:1180	more visible dielectric behavior	1149:1180	more visible dielectric behavior	1149:1180	The PCNF and SiCNF films exhibit more visible dielectric behavior than that of the neat CNF films.					
29859279	1	6	dep	HC	266:267	arg1	pulps					322:326	enzyme treated kraft-NaBH4 pulps	295:326	enzyme treated kraft-NaBH4 pulps	295:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	1	7	theme	kraft-NaBH4	310:320	arg1	pulps					322:326	enzyme treated kraft-NaBH4 pulps	295:326	enzyme treated kraft-NaBH4 pulps	295:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	5	8	theme	Dielectric	580:589	arg1	properties					591:600	Dielectric properties	580:600	Dielectric properties	580:600	Dielectric properties were evaluated using an impedance analyzer.					
29859279	1	9	theme	thermal	139:145	arg1	properties					147:156	the chemical, mechanical, electrical and thermal properties	98:156	the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps	98:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	7	10	theme	lowest	844:849	arg1	value					865:869	the lowest crystallinity value	840:869	the lowest crystallinity value	840:869	The crystallinity values of the SiCNF films calculated from the FT-IR were in agreement with the TGA results, revealing that the lowest crystallinity value was in the SiCNF.					
29859279	1	11	theme	SiCNF	230:234	arg1	films					237:241	silica (SiCNF) films	222:241	silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps	222:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	12	12	theme	films	1402:1406	arg1	properties					1384:1393	the properties	1380:1393	the properties of CNF films having different matrix	1380:1430	Hence, this study evidenced that the properties of CNF films having different matrix are influenced by both the enzymes employed.					
29859279	8	13	theme	lowest	994:999	arg1	values					1001:1006	the lowest values	990:1006	the lowest values	990:1006	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	7	14	theme	crystallinity	851:863	arg1	value					865:869	the lowest crystallinity value	840:869	the lowest crystallinity value	840:869	The crystallinity values of the SiCNF films calculated from the FT-IR were in agreement with the TGA results, revealing that the lowest crystallinity value was in the SiCNF.					
29859279	12	15	contain	having	1408:1413	arg1	films					1402:1406	CNF films	1398:1406	CNF films having different matrix	1398:1430	Hence, this study evidenced that the properties of CNF films having different matrix are influenced by both the enzymes employed.					
29859279	12	15	contain	having	1408:1413	arg2	matrix					1425:1430	different matrix	1415:1430	different matrix	1415:1430	Hence, this study evidenced that the properties of CNF films having different matrix are influenced by both the enzymes employed.					
29859279	11	16	theme	Higher	1215:1220	arg1	stability					1230:1238	Higher thermal stability	1215:1238	Higher thermal stability	1215:1238	Higher thermal stability, thermo-mechanical and mechanical properties were determined in the Pulpzyme HC 2500 enzyme treated films.					
29859279	12	17	theme	CNF	1398:1400	arg1	films					1402:1406	CNF films	1398:1406	CNF films having different matrix	1398:1430	Hence, this study evidenced that the properties of CNF films having different matrix are influenced by both the enzymes employed.					
29859279	1	18	theme	films	237:241	arg1	properties					147:156	the chemical, mechanical, electrical and thermal properties	98:156	the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps	98:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	11	19	theme	Pulpzyme	1308:1315	arg1	films					1340:1344	the Pulpzyme HC 2500 enzyme treated films	1304:1344	the Pulpzyme HC 2500 enzyme treated films	1304:1344	Higher thermal stability, thermo-mechanical and mechanical properties were determined in the Pulpzyme HC 2500 enzyme treated films.					
29859279	10	20	theme	SiCNF	1129:1133	arg1	films					1135:1139	The PCNF and SiCNF films	1116:1139	The PCNF and SiCNF films	1116:1139	The PCNF and SiCNF films exhibit more visible dielectric behavior than that of the neat CNF films.					
29859279	11	21	theme	mechanical	1263:1272	arg1	properties					1274:1283	mechanical properties	1263:1283	mechanical properties	1263:1283	Higher thermal stability, thermo-mechanical and mechanical properties were determined in the Pulpzyme HC 2500 enzyme treated films.					
29859279	1	22	theme	study	69:73	arg1	objective					51:59	The objective	47:59	The objective of this study	47:73	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	6	23	theme	chemical	684:691	arg1	shifts					693:698	chemical shifts	684:698	chemical shifts	684:698	In the PCNF films, new vibrations and chemical shifts were observed.					
29859279	0	24	theme	treated	7:13	arg1	biofilms					19:26	Enzyme treated CNF biofilms	0:26	Enzyme treated CNF biofilms	0:26	Enzyme treated CNF biofilms: Characterization.					
29859279	4	25	theme	Young	482:486	arg1	's					487:488	Young's	482:488	Young's	482:488	Young's and storage moduli of the films were determined via a universal testing machine and DTMA.					
29859279	5	26	theme	impedance	626:634	arg1	analyzer					636:643	an impedance analyzer	623:643	an impedance analyzer	623:643	Dielectric properties were evaluated using an impedance analyzer.					
29859279	1	27	theme	nanofibrillated	161:175	arg1	PCNF					212:215	PCNF	212:215	PCNF	212:215	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	1	27	theme	nanofibrillated	161:175	arg1	alcohol					203:209	nanofibrillated cellulose based polyvinyl alcohol	161:209	nanofibrillated cellulose based polyvinyl alcohol (PCNF)	161:216	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	0	28	theme	Enzyme	0:5	arg1	biofilms					19:26	Enzyme treated CNF biofilms	0:26	Enzyme treated CNF biofilms	0:26	Enzyme treated CNF biofilms: Characterization.					
29859279	8	29	theme	CNF	974:976	arg1	films					978:982	the CNF films	970:982	the neat the CNF films	961:982	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	7	30	theme	TGA	812:814	arg1	results					816:822	the TGA results	808:822	the TGA results	808:822	The crystallinity values of the SiCNF films calculated from the FT-IR were in agreement with the TGA results, revealing that the lowest crystallinity value was in the SiCNF.					
29859279	1	31	theme	cellulose	177:185	arg1	PCNF					212:215	PCNF	212:215	PCNF	212:215	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	1	31	theme	cellulose	177:185	arg1	alcohol					203:209	nanofibrillated cellulose based polyvinyl alcohol	161:209	nanofibrillated cellulose based polyvinyl alcohol (PCNF)	161:216	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	6	32	located	observed	705:712	arg1	films					658:662	the PCNF films	649:662	the PCNF films	649:662	In the PCNF films, new vibrations and chemical shifts were observed.					
29859279	6	32	located	observed	705:712	arg2	shifts					693:698	chemical shifts	684:698	chemical shifts	684:698	In the PCNF films, new vibrations and chemical shifts were observed.					
29859279	6	32	located	observed	705:712	arg2	vibrations					669:678	new vibrations	665:678	new vibrations	665:678	In the PCNF films, new vibrations and chemical shifts were observed.					
29859279	8	33	theme	neat	1029:1032	arg1	films					1040:1044	the neat SiCNF films	1025:1044	the neat SiCNF films	1025:1044	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	7	34	with	agreement	793:801	arg1	results					816:822	the TGA results	808:822	the TGA results	808:822	The crystallinity values of the SiCNF films calculated from the FT-IR were in agreement with the TGA results, revealing that the lowest crystallinity value was in the SiCNF.					
29859279	6	35	theme	new	665:667	arg1	vibrations					669:678	new vibrations	665:678	new vibrations	665:678	In the PCNF films, new vibrations and chemical shifts were observed.					
29859279	1	36	theme	based	187:191	arg1	PCNF					212:215	PCNF	212:215	PCNF	212:215	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	1	36	theme	based	187:191	arg1	alcohol					203:209	nanofibrillated cellulose based polyvinyl alcohol	161:209	nanofibrillated cellulose based polyvinyl alcohol (PCNF)	161:216	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	0	37	theme	CNF	15:17	arg1	biofilms					19:26	Enzyme treated CNF biofilms	0:26	Enzyme treated CNF biofilms	0:26	Enzyme treated CNF biofilms: Characterization.					
29859279	4	38	theme	testing	554:560	arg1	machine					562:568	a universal testing machine	542:568	a universal testing machine	542:568	Young's and storage moduli of the films were determined via a universal testing machine and DTMA.					
29859279	8	39	dep	neat	965:968	arg1	films					978:982	the CNF films	970:982	the neat the CNF films	961:982	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	1	40	theme	polyvinyl	193:201	arg1	PCNF					212:215	PCNF	212:215	PCNF	212:215	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	1	40	theme	polyvinyl	193:201	arg1	alcohol					203:209	nanofibrillated cellulose based polyvinyl alcohol	161:209	nanofibrillated cellulose based polyvinyl alcohol (PCNF)	161:216	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	11	41	theme	HC	1317:1318	arg1	films					1340:1344	the Pulpzyme HC 2500 enzyme treated films	1304:1344	the Pulpzyme HC 2500 enzyme treated films	1304:1344	Higher thermal stability, thermo-mechanical and mechanical properties were determined in the Pulpzyme HC 2500 enzyme treated films.					
29859279	4	42	theme	universal	544:552	arg1	machine					562:568	a universal testing machine	542:568	a universal testing machine	542:568	Young's and storage moduli of the films were determined via a universal testing machine and DTMA.					
29859279	8	43	located	observed	1013:1020	arg1	films					1040:1044	the neat SiCNF films	1025:1044	the neat SiCNF films	1025:1044	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	8	43	located	observed	1013:1020	arg2	values					1001:1006	the lowest values	990:1006	the lowest values	990:1006	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	1	44	theme	alcohol	203:209	arg1	properties					147:156	the chemical, mechanical, electrical and thermal properties	98:156	the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps	98:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	3	45	theme	chemical	399:406	arg1	characterization					408:423	chemical characterization	399:423	chemical characterization	399:423	Thermal stability, chemical characterization and crystallization were determined using TGA and FT-IR.					
29859279	4	46	theme	films	516:520	arg1	's					487:488	Young's	482:488	Young's	482:488	Young's and storage moduli of the films were determined via a universal testing machine and DTMA.					
29859279	4	46	theme	films	516:520	arg1	moduli					502:507	storage moduli	494:507	storage moduli	494:507	Young's and storage moduli of the films were determined via a universal testing machine and DTMA.					
29859279	7	47	theme	crystallinity	719:731	arg1	values					733:738	The crystallinity values	715:738	The crystallinity values of the SiCNF films calculated from the FT-IR	715:783	The crystallinity values of the SiCNF films calculated from the FT-IR were in agreement with the TGA results, revealing that the lowest crystallinity value was in the SiCNF.					
29859279	9	48	theme	SiCNF	1060:1064	arg1	films					1066:1070	the SiCNF films	1056:1070	the SiCNF films	1056:1070	However, the SiCNF films revealed higher thermo-mechanical property.					
29859279	8	49	theme	higher	893:898	arg1	modulus					906:912	The higher Young modulus	889:912	The higher Young modulus	889:912	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	3	50	theme	Thermal	380:386	arg1	stability					388:396	Thermal stability	380:396	Thermal stability	380:396	Thermal stability, chemical characterization and crystallization were determined using TGA and FT-IR.					
29859279	0	51	dep	Characterization	29:44	arg1	biofilms					19:26	Enzyme treated CNF biofilms	0:26	Enzyme treated CNF biofilms	0:26	Enzyme treated CNF biofilms: Characterization.					
29859279	8	52	theme	SiCNF	1034:1038	arg1	films					1040:1044	the neat SiCNF films	1025:1044	the neat SiCNF films	1025:1044	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	7	53	theme	films	753:757	arg1	values					733:738	The crystallinity values	715:738	The crystallinity values of the SiCNF films calculated from the FT-IR	715:783	The crystallinity values of the SiCNF films calculated from the FT-IR were in agreement with the TGA results, revealing that the lowest crystallinity value was in the SiCNF.					
29859279	4	54	theme	storage	494:500	arg1	moduli					502:507	storage moduli	494:507	storage moduli	494:507	Young's and storage moduli of the films were determined via a universal testing machine and DTMA.					
29859279	1	55	theme	chemical	102:109	arg1	properties					147:156	the chemical, mechanical, electrical and thermal properties	98:156	the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps	98:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	6	56	theme	PCNF	653:656	arg1	films					658:662	the PCNF films	649:662	the PCNF films	649:662	In the PCNF films, new vibrations and chemical shifts were observed.					
29859279	12	57	theme	different	1415:1423	arg1	matrix					1425:1430	different matrix	1415:1430	different matrix	1415:1430	Hence, this study evidenced that the properties of CNF films having different matrix are influenced by both the enzymes employed.					
29859279	8	58	theme	Young	900:904	arg1	modulus					906:912	The higher Young modulus	889:912	The higher Young modulus	889:912	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	11	59	theme	treated	1332:1338	arg1	films					1340:1344	the Pulpzyme HC 2500 enzyme treated films	1304:1344	the Pulpzyme HC 2500 enzyme treated films	1304:1344	Higher thermal stability, thermo-mechanical and mechanical properties were determined in the Pulpzyme HC 2500 enzyme treated films.					
29859279	10	60	theme	visible	1154:1160	arg1	behavior					1173:1180	more visible dielectric behavior	1149:1180	more visible dielectric behavior	1149:1180	The PCNF and SiCNF films exhibit more visible dielectric behavior than that of the neat CNF films.					
29859279	10	61	theme	neat	1199:1202	arg1	films					1208:1212	the neat CNF films	1195:1212	the neat CNF films	1195:1212	The PCNF and SiCNF films exhibit more visible dielectric behavior than that of the neat CNF films.					
29859279	9	62	theme	higher	1081:1086	arg1	property					1106:1113	higher thermo-mechanical property	1081:1113	higher thermo-mechanical property	1081:1113	However, the SiCNF films revealed higher thermo-mechanical property.					
29859279	8	63	from	value	938:942	arg1	elongation					918:927	elongation	918:927	elongation at break value	918:942	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	8	63	from	value	938:942	arg1	modulus					906:912	The higher Young modulus	889:912	The higher Young modulus	889:912	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	9	64	theme	thermo-mechanical	1088:1104	arg1	property					1106:1113	higher thermo-mechanical property	1081:1113	higher thermo-mechanical property	1081:1113	However, the SiCNF films revealed higher thermo-mechanical property.					
29859279	1	65	theme	mechanical	112:121	arg1	properties					147:156	the chemical, mechanical, electrical and thermal properties	98:156	the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps	98:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	7	66	theme	SiCNF	747:751	arg1	films					753:757	the SiCNF films	743:757	the SiCNF films	743:757	The crystallinity values of the SiCNF films calculated from the FT-IR were in agreement with the TGA results, revealing that the lowest crystallinity value was in the SiCNF.					
29859279	1	67	theme	enzyme	295:300	arg1	pulps					322:326	enzyme treated kraft-NaBH4 pulps	295:326	enzyme treated kraft-NaBH4 pulps	295:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	10	68	theme	PCNF	1120:1123	arg1	films					1135:1139	The PCNF and SiCNF films	1116:1139	The PCNF and SiCNF films	1116:1139	The PCNF and SiCNF films exhibit more visible dielectric behavior than that of the neat CNF films.					
29859279	8	69	theme	break	932:936	arg1	value					938:942	break value	932:942	break value	932:942	The higher Young modulus and elongation at break value were obtained in the neat the CNF films while the lowest values were observed in the neat SiCNF films.					
29859279	1	70	theme	electrical	124:133	arg1	properties					147:156	the chemical, mechanical, electrical and thermal properties	98:156	the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps	98:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
29859279	1	71	theme	silica	222:227	arg1	films					237:241	silica (SiCNF) films	222:241	silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps	222:326	The objective of this study was to characterize on the chemical, mechanical, electrical and thermal properties of nanofibrillated cellulose based polyvinyl alcohol (PCNF) and silica (SiCNF) films obtained from Pulpzyme HC 2500 and Celluclast 1.5 L enzyme treated kraft-NaBH4 pulps.					
31129204	0	0	theme	mulberry	87:94	arg1	extract					109:115	mulberry polyphenolic extract	87:115	mulberry polyphenolic extract	87:115	Preparation of pH-sensitive and antioxidant packaging films based on κ-carrageenan and mulberry polyphenolic extract.					
31129204	9	1	theme	κ-carrageenan-MPE	1356:1372	arg1	films					1374:1378	κ-carrageenan-MPE films	1356:1378	κ-carrageenan-MPE films	1356:1378	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	9	1	theme	κ-carrageenan-MPE	1356:1372	arg1	materials					1441:1449	novel active and intelligent food packaging materials	1397:1449	novel active and intelligent food packaging materials	1397:1449	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	4	2	theme	antioxidant	671:681	arg1	activity					683:690	antioxidant activity	671:690	antioxidant activity	671:690	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	9	3	theme	active	1403:1408	arg1	films					1374:1378	κ-carrageenan-MPE films	1356:1378	κ-carrageenan-MPE films	1356:1378	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	9	3	theme	active	1403:1408	arg1	materials					1441:1449	novel active and intelligent food packaging materials	1397:1449	novel active and intelligent food packaging materials	1397:1449	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	1	4	theme	κ-carrageenan	162:174	arg1	films					182:186	antioxidant and pH-sensitive κ-carrageenan based films	133:186	antioxidant and pH-sensitive κ-carrageenan based films	133:186	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	9	5	theme	food	1426:1429	arg1	films					1374:1378	κ-carrageenan-MPE films	1356:1378	κ-carrageenan-MPE films	1356:1378	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	9	5	theme	food	1426:1429	arg1	materials					1441:1449	novel active and intelligent food packaging materials	1397:1449	novel active and intelligent food packaging materials	1397:1449	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	5	6	theme	moisture	754:761	arg1	content					763:769	the moisture content	750:769	the moisture content	750:769	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	7	7	theme	infrared	1131:1138	arg1	spectroscopy					1140:1151	infrared spectroscopy	1131:1151	infrared spectroscopy	1131:1151	The existence of intermolecular interactions between MPE and κ-carrageenan matrix was confirmed by infrared spectroscopy and X-ray diffraction.					
31129204	8	8	theme	κ-carrageenan-MPE	1223:1239	arg1	films					1241:1245	κ-carrageenan-MPE films	1223:1245	κ-carrageenan-MPE films	1223:1245	When applied to monitor the freshness of milk, κ-carrageenan-MPE films showed evident color changes as milk deteriorated after storage at 40 °C for 8 h.					
31129204	1	9	theme	based	176:180	arg1	films					182:186	antioxidant and pH-sensitive κ-carrageenan based films	133:186	antioxidant and pH-sensitive κ-carrageenan based films	133:186	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	6	10	theme	rougher	978:984	arg1	surfaces					986:993	heterogeneous and rougher surfaces	960:993	heterogeneous and rougher surfaces	960:993	In addition, κ-carrageenan-MPE films exhibited heterogeneous and rougher surfaces as compared with κ-carrageenan film.					
31129204	0	11	theme	polyphenolic	96:107	arg1	extract					109:115	mulberry polyphenolic extract	87:115	mulberry polyphenolic extract	87:115	Preparation of pH-sensitive and antioxidant packaging films based on κ-carrageenan and mulberry polyphenolic extract.					
31129204	6	12	theme	heterogeneous	960:972	arg1	surfaces					986:993	heterogeneous and rougher surfaces	960:993	heterogeneous and rougher surfaces	960:993	In addition, κ-carrageenan-MPE films exhibited heterogeneous and rougher surfaces as compared with κ-carrageenan film.					
31129204	9	13	used	used	1389:1392	arg2	materials					1441:1449	novel active and intelligent food packaging materials	1397:1449	novel active and intelligent food packaging materials	1397:1449	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	9	13	used	used	1389:1392	arg2	films					1374:1378	κ-carrageenan-MPE films	1356:1378	κ-carrageenan-MPE films	1356:1378	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	2	14	theme	anthocyanin	294:304	arg1	content					306:312	abundant anthocyanin content	285:312	abundant anthocyanin content	285:312	Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13.					
31129204	1	15	theme	antioxidant	133:143	arg1	films					182:186	antioxidant and pH-sensitive κ-carrageenan based films	133:186	antioxidant and pH-sensitive κ-carrageenan based films	133:186	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	7	16	theme	κ-carrageenan	1093:1105	arg1	matrix					1107:1112	κ-carrageenan matrix	1093:1112	κ-carrageenan matrix	1093:1112	The existence of intermolecular interactions between MPE and κ-carrageenan matrix was confirmed by infrared spectroscopy and X-ray diffraction.					
31129204	4	17	theme	κ-carrageenan	721:733	arg1	film					735:738	κ-carrageenan film	721:738	κ-carrageenan film	721:738	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	2	18	theme	abundant	285:292	arg1	content					306:312	abundant anthocyanin content	285:312	abundant anthocyanin content	285:312	Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13.					
31129204	4	19	theme	tensile	634:640	arg1	strength					642:649	tensile strength	634:649	tensile strength	634:649	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	5	20	theme	vapor	778:782	arg1	permeability					784:795	water vapor permeability	772:795	water vapor permeability	772:795	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	7	21	theme	X-ray	1157:1161	arg1	diffraction					1163:1173	X-ray diffraction	1157:1173	X-ray diffraction	1157:1173	The existence of intermolecular interactions between MPE and κ-carrageenan matrix was confirmed by infrared spectroscopy and X-ray diffraction.					
31129204	3	22	theme	blue	529:532	arg1	color					534:538	blue color	529:538	blue color	529:538	When MPE was incorporated into κ-carrageenan film, the composite films presented blue color.					
31129204	2	23	theme	final	399:403	arg1	gray					405:408	final gray	399:408	final gray	399:408	Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13.					
31129204	2	24	dep	13	444:445	arg1	to					441:442	to	441:442	to	441:442	Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13.					
31129204	2	25	theme	pH-sensitive	326:337	arg1	property					339:346	pH-sensitive property	326:346	pH-sensitive property with solution color	326:366	Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13.					
31129204	6	26	theme	κ-carrageenan	1012:1024	arg1	film					1026:1029	κ-carrageenan film	1012:1029	κ-carrageenan film	1012:1029	In addition, κ-carrageenan-MPE films exhibited heterogeneous and rougher surfaces as compared with κ-carrageenan film.					
31129204	4	27	theme	pH-sensitive	696:707	arg1	property					709:716	pH-sensitive property	696:716	pH-sensitive property	696:716	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	8	28	dep	color	1262:1266	arg1	evident					1254:1260	evident	1254:1260	evident	1254:1260	When applied to monitor the freshness of milk, κ-carrageenan-MPE films showed evident color changes as milk deteriorated after storage at 40 °C for 8 h.					
31129204	8	29	theme	color	1262:1266	arg1	changes					1268:1274	evident color changes	1254:1274	evident color changes	1254:1274	When applied to monitor the freshness of milk, κ-carrageenan-MPE films showed evident color changes as milk deteriorated after storage at 40 °C for 8 h.					
31129204	4	30	theme	%	581:581	arg1	incorporation					554:566	the incorporation	550:566	the incorporation of 2 and 4 wt% of MPE	550:588	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	7	31	theme	interactions	1064:1075	arg1	existence					1036:1044	The existence	1032:1044	The existence of intermolecular interactions between MPE and κ-carrageenan matrix	1032:1112	The existence of intermolecular interactions between MPE and κ-carrageenan matrix was confirmed by infrared spectroscopy and X-ray diffraction.					
31129204	0	32	theme	pH-sensitive	15:26	arg1	films					54:58	pH-sensitive and antioxidant packaging films	15:58	pH-sensitive and antioxidant packaging films	15:58	Preparation of pH-sensitive and antioxidant packaging films based on κ-carrageenan and mulberry polyphenolic extract.					
31129204	6	33	theme	κ-carrageenan-MPE	926:942	arg1	films					944:948	κ-carrageenan-MPE films	926:948	κ-carrageenan-MPE films	926:948	In addition, κ-carrageenan-MPE films exhibited heterogeneous and rougher surfaces as compared with κ-carrageenan film.					
31129204	4	34	theme	film	735:738	arg1	thickness					623:631	the thickness	619:631	the thickness	619:631	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	4	34	theme	film	735:738	arg1	strength					642:649	tensile strength	634:649	tensile strength	634:649	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	4	34	theme	film	735:738	arg1	stability					660:668	thermal stability	652:668	thermal stability	652:668	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	4	34	theme	film	735:738	arg1	activity					683:690	antioxidant activity	671:690	antioxidant activity	671:690	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	4	34	theme	film	735:738	arg1	property					709:716	pH-sensitive property	696:716	pH-sensitive property	696:716	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	1	35	theme	different	220:228	arg1	extract					263:269	mulberry polyphenolic extract	241:269	mulberry polyphenolic extract (MPE)	241:275	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	1	35	theme	different	220:228	arg1	amounts					230:236	different amounts	220:236	different amounts of mulberry polyphenolic extract (MPE)	220:275	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	1	35	theme	different	220:228	arg1	MPE					272:274	MPE	272:274	MPE	272:274	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	0	36	theme	packaging	44:52	arg1	films					54:58	pH-sensitive and antioxidant packaging films	15:58	pH-sensitive and antioxidant packaging films	15:58	Preparation of pH-sensitive and antioxidant packaging films based on κ-carrageenan and mulberry polyphenolic extract.					
31129204	2	37	with	property	339:346	arg1	color					362:366	solution color	353:366	solution color	353:366	Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13.					
31129204	2	38	theme	pH	415:416	arg1	value					418:422	pH value	415:422	pH value	415:422	Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13.					
31129204	0	39	theme	antioxidant	32:42	arg1	films					54:58	pH-sensitive and antioxidant packaging films	15:58	pH-sensitive and antioxidant packaging films	15:58	Preparation of pH-sensitive and antioxidant packaging films based on κ-carrageenan and mulberry polyphenolic extract.					
31129204	7	40	theme	intermolecular	1049:1062	arg1	interactions					1064:1075	intermolecular interactions	1049:1075	intermolecular interactions between MPE and κ-carrageenan matrix	1049:1112	The existence of intermolecular interactions between MPE and κ-carrageenan matrix was confirmed by infrared spectroscopy and X-ray diffraction.					
31129204	5	41	theme	UV-vis	798:803	arg1	transmittance					811:823	UV-vis light transmittance	798:823	UV-vis light transmittance	798:823	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	5	42	theme	water	772:776	arg1	permeability					784:795	water vapor permeability	772:795	water vapor permeability	772:795	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	8	43	theme	milk	1217:1220	arg1	freshness					1204:1212	the freshness	1200:1212	the freshness of milk	1200:1220	When applied to monitor the freshness of milk, κ-carrageenan-MPE films showed evident color changes as milk deteriorated after storage at 40 °C for 8 h.					
31129204	3	44	theme	κ-carrageenan	479:491	arg1	film					493:496	κ-carrageenan film	479:496	κ-carrageenan film	479:496	When MPE was incorporated into κ-carrageenan film, the composite films presented blue color.					
31129204	0	45	theme	films	54:58	arg1	Preparation					0:10	Preparation	0:10	Preparation of pH-sensitive and antioxidant packaging films	0:58	Preparation of pH-sensitive and antioxidant packaging films based on κ-carrageenan and mulberry polyphenolic extract.					
31129204	2	46	theme	solution	353:360	arg1	color					362:366	solution color	353:366	solution color	353:366	Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13.					
31129204	4	47	theme	thermal	652:658	arg1	stability					660:668	thermal stability	652:668	thermal stability	652:668	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	5	48	theme	MPE	890:892	arg1	MPE					890:892	MPE	890:892	MPE	890:892	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	5	48	theme	MPE	890:892	arg1	%					885:885	2 and 4 wt%	875:885	2 and 4 wt% of MPE	875:892	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	9	49	theme	novel	1397:1401	arg1	films					1374:1378	κ-carrageenan-MPE films	1356:1378	κ-carrageenan-MPE films	1356:1378	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	9	49	theme	novel	1397:1401	arg1	materials					1441:1449	novel active and intelligent food packaging materials	1397:1449	novel active and intelligent food packaging materials	1397:1449	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	1	50	theme	mulberry	241:248	arg1	extract					263:269	mulberry polyphenolic extract	241:269	mulberry polyphenolic extract (MPE)	241:275	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	1	50	theme	mulberry	241:248	arg1	MPE					272:274	MPE	272:274	MPE	272:274	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	9	51	theme	packaging	1431:1439	arg1	films					1374:1378	κ-carrageenan-MPE films	1356:1378	κ-carrageenan-MPE films	1356:1378	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	9	51	theme	packaging	1431:1439	arg1	materials					1441:1449	novel active and intelligent food packaging materials	1397:1449	novel active and intelligent food packaging materials	1397:1449	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	2	52	dep	showed	319:324	arg1	changed					368:374	changed	368:374	showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13	319:445	Due to abundant anthocyanin content, MPE showed pH-sensitive property with solution color changed from red to purple and final gray when pH value increased from 2 to 13.					
31129204	1	53	theme	polyphenolic	250:261	arg1	extract					263:269	mulberry polyphenolic extract	241:269	mulberry polyphenolic extract (MPE)	241:275	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	1	53	theme	polyphenolic	250:261	arg1	MPE					272:274	MPE	272:274	MPE	272:274	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	3	54	theme	composite	503:511	arg1	films					513:517	the composite films	499:517	the composite films	499:517	When MPE was incorporated into κ-carrageenan film, the composite films presented blue color.					
31129204	1	55	theme	extract	263:269	arg1	extract					263:269	mulberry polyphenolic extract	241:269	mulberry polyphenolic extract (MPE)	241:275	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	1	55	theme	extract	263:269	arg1	amounts					230:236	different amounts	220:236	different amounts of mulberry polyphenolic extract (MPE)	220:275	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	1	55	theme	extract	263:269	arg1	MPE					272:274	MPE	272:274	MPE	272:274	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	5	56	theme	light	805:809	arg1	transmittance					811:823	UV-vis light transmittance	798:823	UV-vis light transmittance	798:823	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	5	57	from	break	843:847	arg1	elongation					829:838	elongation	829:838	elongation	829:838	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	5	57	from	break	843:847	arg1	content					763:769	the moisture content	750:769	the moisture content	750:769	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	5	57	from	break	843:847	arg1	transmittance					811:823	UV-vis light transmittance	798:823	UV-vis light transmittance	798:823	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	5	57	from	break	843:847	arg1	permeability					784:795	water vapor permeability	772:795	water vapor permeability	772:795	However, the moisture content, water vapor permeability, UV-vis light transmittance and elongation at break were greatly reduced when 2 and 4 wt% of MPE were incorporated.					
31129204	1	58	theme	pH-sensitive	149:160	arg1	films					182:186	antioxidant and pH-sensitive κ-carrageenan based films	133:186	antioxidant and pH-sensitive κ-carrageenan based films	133:186	In this study, antioxidant and pH-sensitive κ-carrageenan based films were developed by incorporating different amounts of mulberry polyphenolic extract (MPE).					
31129204	9	59	theme	intelligent	1414:1424	arg1	films					1374:1378	κ-carrageenan-MPE films	1356:1378	κ-carrageenan-MPE films	1356:1378	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	9	59	theme	intelligent	1414:1424	arg1	materials					1441:1449	novel active and intelligent food packaging materials	1397:1449	novel active and intelligent food packaging materials	1397:1449	Our results suggested that κ-carrageenan-MPE films could be used as novel active and intelligent food packaging materials.					
31129204	4	60	theme	MPE	586:588	arg1	MPE					586:588	MPE	586:588	MPE	586:588	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
31129204	4	60	theme	MPE	586:588	arg1	%					581:581	2 and 4 wt%	571:581	2 and 4 wt% of MPE	571:588	Notably, the incorporation of 2 and 4 wt% of MPE could significantly increase the thickness, tensile strength, thermal stability, antioxidant activity and pH-sensitive property of κ-carrageenan film.					
30483266	7	0	from	offspring	907:915	arg1	group					925:929	MHFD group	920:929	MHFD group	920:929	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	2	1	theme	offspring	327:335	arg1	susceptibility					309:322	the susceptibility	305:322	the susceptibility of offspring to colitis in adulthood	305:359	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	9	2	theme	H&E	1251:1253	arg1	staining					1255:1262	H&E staining	1251:1262	H&E staining	1251:1262	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	11	3	theme	bacterial	1789:1797	arg1	diversity					1799:1807	the bacterial diversity	1785:1807	the bacterial diversity	1785:1807	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	2	4	theme	high	281:284	arg1	MHFD					296:299	MHFD	296:299	MHFD	296:299	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	2	4	theme	high	281:284	arg1	diet					290:293	maternal high fat diet	272:293	maternal high fat diet (MHFD)	272:300	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	3	5	theme	control	468:474	arg1	diet					476:479	control diet [10 E% fat	468:490	control diet [10 E% fat	468:490	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	5	theme	control	468:474	arg1	diet					510:513	maternal control diet	493:513	maternal control diet	493:513	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	5	theme	control	468:474	arg1	MCD					516:518	MCD	516:518	MCD	516:518	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	9	6	theme	obvious	1274:1280	arg1	inflammation					1294:1305	no obvious microscopic inflammation	1271:1305	no obvious microscopic inflammation	1271:1305	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	13	7	theme	mucosal	2190:2196	arg1	barrier					2198:2204	intestinal mucosal barrier	2179:2204	intestinal mucosal barrier	2179:2204	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	2	8	from	colitis	340:346	arg1	adulthood					351:359	adulthood	351:359	adulthood	351:359	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	8	9	theme	disrupted	1190:1198	arg1	function					1208:1215	disrupted barrier function	1190:1215	disrupted barrier function	1190:1215	Compared with MCD group, MHFD significantly inhibited intestinal development and disrupted barrier function in 3-week old offspring.					
30483266	11	10	theme	diet	1657:1660	arg1	consumption					1634:1644	5 weeks consumption	1626:1644	5 weeks consumption of control diet in both groups	1626:1675	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	3	11	theme	%	486:486	arg1	fat					488:490	E% fat	485:490	control diet [10 E% fat	468:490	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	1	12	theme	inflammatory	194:205	arg1	disease					213:219	inflammatory bowel disease	194:219	inflammatory bowel disease	194:219	Background: Accumulating evidence shows that high fat diet is closely associated with inflammatory bowel disease.					
30483266	6	13	theme	DSS	828:830	arg1	solution					832:839	2% DSS solution	825:839	2% DSS solution	825:839	Offspring were also treated with 2% DSS solution for 5 days and the severity of colitis was assessed.					
30483266	12	14	theme	colitis	1950:1956	arg1	severity					1938:1945	the severity	1934:1945	the severity of colitis	1934:1956	Finally, after DSS treatment in 8-week old offspring, MHFD significantly exacerbated the severity of colitis and increased the production of proinflammatory cytokine.					
30483266	11	15	theme	microbiota	1682:1691	arg1	different					1742:1750	different	1742:1750	different	1742:1750	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	11	15	theme	microbiota	1682:1691	arg1	composition					1693:1703	the microbiota composition	1678:1703	the microbiota composition of offspring in MHFD group	1678:1730	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	6	16	theme	2	825:825	arg1	%					826:826	%	826:826	%	826:826	Offspring were also treated with 2% DSS solution for 5 days and the severity of colitis was assessed.					
30483266	10	17	theme	fecal	1464:1468	arg1	analysis					1470:1477	fecal analysis	1464:1477	fecal analysis of the 3-week old offspring	1464:1505	Moreover, fecal analysis of the 3-week old offspring indicated that the microbiota compositions and diversity were significantly changed in MHFD group.					
30483266	3	18	theme	control	502:508	arg1	diet					510:513	maternal control diet	493:513	maternal control diet	493:513	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	18	theme	control	502:508	arg1	diet					476:479	control diet [10 E% fat	468:490	control diet [10 E% fat	468:490	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	9	19	theme	old	1332:1334	arg1	offspring					1336:1344	3-week old offspring	1325:1344	3-week old offspring	1325:1344	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	13	20	theme	intestinal	2081:2090	arg1	development					2092:2102	intestinal development	2081:2102	intestinal development	2081:2102	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	8	21	theme	MCD	1123:1125	arg1	group					1127:1131	MCD group	1123:1131	MCD group	1123:1131	Compared with MCD group, MHFD significantly inhibited intestinal development and disrupted barrier function in 3-week old offspring.					
30483266	2	22	theme	underlying	247:256	arg1	mechanisms					258:267	underlying mechanisms	247:267	underlying mechanisms	247:267	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	1	23	theme	Accumulating	120:131	arg1	evidence					133:140	Accumulating evidence	120:140	Accumulating evidence	120:140	Background: Accumulating evidence shows that high fat diet is closely associated with inflammatory bowel disease.					
30483266	0	24	from	Colitis	86:92	arg1	Adulthood					97:105	Adulthood	97:105	Adulthood	97:105	Maternal High Fat Diet Alters Gut Microbiota of Offspring and Exacerbates DSS-Induced Colitis in Adulthood.					
30483266	11	25	from	that	1757:1760	arg1	composition					1693:1703	the microbiota composition	1678:1703	the microbiota composition of offspring in MHFD group	1678:1730	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	11	25	from	that	1757:1760	arg1	different					1742:1750	different	1742:1750	different	1742:1750	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	4	26	theme	mucosal	588:594	arg1	function					604:611	mucosal barrier function	588:611	mucosal barrier function	588:611	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	0	27	theme	Offspring	48:56	arg1	Microbiota					34:43	Gut Microbiota	30:43	Gut Microbiota of Offspring	30:56	Maternal High Fat Diet Alters Gut Microbiota of Offspring and Exacerbates DSS-Induced Colitis in Adulthood.					
30483266	7	28	theme	age	1003:1005	arg1	weeks					994:998	2-4 weeks	990:998	2-4 weeks of age	990:1005	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	13	29	from	MHFD	2050:2053	arg1	life					2064:2067	early life	2058:2067	early life	2058:2067	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	1	30	theme	fat	158:160	arg1	diet					162:165	high fat diet	153:165	high fat diet	153:165	Background: Accumulating evidence shows that high fat diet is closely associated with inflammatory bowel disease.					
30483266	4	31	theme	offspring	665:673	arg1	microbiota					614:623	microbiota	614:623	microbiota	614:623	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	4	31	theme	offspring	665:673	arg1	function					604:611	mucosal barrier function	588:611	mucosal barrier function	588:611	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	4	31	theme	offspring	665:673	arg1	inflammation					638:649	mucosal inflammation	630:649	mucosal inflammation	630:649	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	4	31	theme	offspring	665:673	arg1	development					575:585	The intestinal development	560:585	The intestinal development	560:585	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	11	32	theme	weeks	1628:1632	arg1	consumption					1634:1644	5 weeks consumption	1626:1644	5 weeks consumption of control diet in both groups	1626:1675	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	8	33	theme	barrier	1200:1206	arg1	function					1208:1215	disrupted barrier function	1190:1215	disrupted barrier function	1190:1215	Compared with MCD group, MHFD significantly inhibited intestinal development and disrupted barrier function in 3-week old offspring.					
30483266	10	34	theme	old	1493:1495	arg1	offspring					1497:1505	the 3-week old offspring	1482:1505	the 3-week old offspring	1482:1505	Moreover, fecal analysis of the 3-week old offspring indicated that the microbiota compositions and diversity were significantly changed in MHFD group.					
30483266	0	35	theme	Maternal	0:7	arg1	Diet					18:21	Maternal High Fat Diet	0:21	Maternal High Fat Diet	0:21	Maternal High Fat Diet Alters Gut Microbiota of Offspring and Exacerbates DSS-Induced Colitis in Adulthood.					
30483266	13	36	theme	early	2058:2062	arg1	life					2064:2067	early life	2058:2067	early life	2058:2067	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	4	37	theme	3-week	654:659	arg1	offspring					665:673	3-week old offspring	654:673	3-week old offspring	654:673	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	13	38	from	colitis	2244:2250	arg1	adulthood					2255:2263	adulthood	2255:2263	adulthood	2255:2263	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	0	39	theme	Fat	14:16	arg1	Diet					18:21	Maternal High Fat Diet	0:21	Maternal High Fat Diet	0:21	Maternal High Fat Diet Alters Gut Microbiota of Offspring and Exacerbates DSS-Induced Colitis in Adulthood.					
30483266	13	40	theme	intestinal	2179:2188	arg1	barrier					2198:2204	intestinal mucosal barrier	2179:2204	intestinal mucosal barrier	2179:2204	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	9	41	theme	MHFD	1442:1445	arg1	group					1447:1451	MHFD group	1442:1451	MHFD group	1442:1451	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	2	42	from	mechanisms	258:267	arg1	susceptibility					309:322	the susceptibility	305:322	the susceptibility of offspring to colitis in adulthood	305:359	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	12	43	theme	8-week	1881:1886	arg1	offspring					1892:1900	8-week old offspring	1881:1900	8-week old offspring	1881:1900	Finally, after DSS treatment in 8-week old offspring, MHFD significantly exacerbated the severity of colitis and increased the production of proinflammatory cytokine.					
30483266	3	44	theme	C57BL/6	390:396	arg1	mice					407:410	C57BL/6 pregnant mice	390:410	C57BL/6 pregnant mice	390:410	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	8	45	theme	old	1227:1229	arg1	offspring					1231:1239	3-week old offspring	1220:1239	3-week old offspring	1220:1239	Compared with MCD group, MHFD significantly inhibited intestinal development and disrupted barrier function in 3-week old offspring.					
30483266	3	46	theme	%	446:446	arg1	fat					448:450	60 E% fat	442:450	60 E% fat	442:450	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	46	theme	%	446:446	arg1	group					458:462	MHFD group	453:462	MHFD group	453:462	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	12	47	theme	proinflammatory	1990:2004	arg1	cytokine					2006:2013	proinflammatory cytokine	1990:2013	proinflammatory cytokine	1990:2013	Finally, after DSS treatment in 8-week old offspring, MHFD significantly exacerbated the severity of colitis and increased the production of proinflammatory cytokine.					
30483266	11	48	theme	age	1844:1846	arg1	weeks					1835:1839	8 weeks	1833:1839	8 weeks of age	1833:1846	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	7	49	theme	measured	1087:1094	arg1	points					1101:1106	other measured time points	1081:1106	other measured time points	1081:1106	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	12	50	from	treatment	1868:1876	arg1	offspring					1892:1900	8-week old offspring	1881:1900	8-week old offspring	1881:1900	Finally, after DSS treatment in 8-week old offspring, MHFD significantly exacerbated the severity of colitis and increased the production of proinflammatory cytokine.					
30483266	12	51	theme	old	1888:1890	arg1	offspring					1892:1900	8-week old offspring	1881:1900	8-week old offspring	1881:1900	Finally, after DSS treatment in 8-week old offspring, MHFD significantly exacerbated the severity of colitis and increased the production of proinflammatory cytokine.					
30483266	7	52	theme	MCD	972:974	arg1	group					976:980	MCD group	972:980	MCD group	972:980	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	12	53	theme	DSS	1864:1866	arg1	treatment					1868:1876	DSS treatment	1864:1876	DSS treatment in 8-week old offspring	1864:1900	Finally, after DSS treatment in 8-week old offspring, MHFD significantly exacerbated the severity of colitis and increased the production of proinflammatory cytokine.					
30483266	2	54	theme	fat	286:288	arg1	MHFD					296:299	MHFD	296:299	MHFD	296:299	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	2	54	theme	fat	286:288	arg1	diet					290:293	maternal high fat diet	272:293	maternal high fat diet (MHFD)	272:300	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	11	55	from	composition	1693:1703	arg1	group					1726:1730	MHFD group	1721:1730	MHFD group	1721:1730	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	2	56	theme	maternal	272:279	arg1	MHFD					296:299	MHFD	296:299	MHFD	296:299	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	2	56	theme	maternal	272:279	arg1	diet					290:293	maternal high fat diet	272:293	maternal high fat diet (MHFD)	272:300	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	5	57	theme	age	754:756	arg1	weeks					745:749	8 weeks	743:749	8 weeks of age	743:756	After weaning all mice were fed a control diet until 8 weeks of age when the microbiota was analyzed.					
30483266	2	58	dep	effects	235:241	arg1	the					231:233	the	231:233	the	231:233	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	9	59	theme	microscopic	1282:1292	arg1	inflammation					1294:1305	no obvious microscopic inflammation	1271:1305	no obvious microscopic inflammation	1271:1305	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	13	60	theme	low-grade	2126:2134	arg1	inflammation					2136:2147	low-grade inflammation	2126:2147	low-grade inflammation	2126:2147	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	4	61	theme	intestinal	564:573	arg1	development					575:585	The intestinal development	560:585	The intestinal development	560:585	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	7	62	theme	MHFD	920:923	arg1	group					925:929	MHFD group	920:929	MHFD group	920:929	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	3	63	theme	E	485:485	arg1	fat					488:490	E% fat	485:490	control diet [10 E% fat	468:490	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	64	dep	MCD	516:518	arg1	group					521:525	group	521:525	group	521:525	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	11	65	from	consumption	1634:1644	arg1	groups					1670:1675	both groups	1665:1675	both groups	1665:1675	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	9	66	dep	induced	1431:1437	arg1	indicated					1394:1402	indicated	1394:1402	indicated	1394:1402	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	9	67	theme	inflammatory	1371:1382	arg1	cytokines					1384:1392	inflammatory cytokines	1371:1392	inflammatory cytokines	1371:1392	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	1	68	theme	bowel	207:211	arg1	disease					213:219	inflammatory bowel disease	194:219	inflammatory bowel disease	194:219	Background: Accumulating evidence shows that high fat diet is closely associated with inflammatory bowel disease.					
30483266	6	69	theme	%	826:826	arg1	solution					832:839	2% DSS solution	825:839	2% DSS solution	825:839	Offspring were also treated with 2% DSS solution for 5 days and the severity of colitis was assessed.					
30483266	3	70	theme	maternal	493:500	arg1	diet					510:513	maternal control diet	493:513	maternal control diet	493:513	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	70	theme	maternal	493:500	arg1	diet					476:479	control diet [10 E% fat	468:490	control diet [10 E% fat	468:490	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	9	71	theme	3-week	1325:1330	arg1	offspring					1336:1344	3-week old offspring	1325:1344	3-week old offspring	1325:1344	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	7	72	from	points	1101:1106	arg1	groups					1071:1076	two groups	1067:1076	two groups at other measured time points	1067:1106	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	9	73	theme	offspring	1336:1344	arg1	offspring					1336:1344	3-week old offspring	1325:1344	3-week old offspring	1325:1344	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	9	73	theme	offspring	1336:1344	arg1	groups					1315:1320	both groups	1310:1320	both groups of 3-week old offspring	1310:1344	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	8	74	theme	intestinal	1163:1172	arg1	development					1174:1184	intestinal development	1163:1184	intestinal development	1163:1184	Compared with MCD group, MHFD significantly inhibited intestinal development and disrupted barrier function in 3-week old offspring.					
30483266	7	75	theme	body	1047:1050	arg1	weight					1052:1057	the body weight	1043:1057	the body weight between two groups at other measured time points	1043:1106	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	5	76	theme	control	724:730	arg1	diet					732:735	a control diet	722:735	a control diet	722:735	After weaning all mice were fed a control diet until 8 weeks of age when the microbiota was analyzed.					
30483266	9	77	theme	increased	1347:1355	arg1	production					1357:1366	increased production	1347:1366	increased production of inflammatory cytokines	1347:1392	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	6	78	theme	colitis	872:878	arg1	severity					860:867	the severity	856:867	the severity of colitis	856:878	Offspring were also treated with 2% DSS solution for 5 days and the severity of colitis was assessed.					
30483266	0	79	theme	DSS-Induced	74:84	arg1	Colitis					86:92	DSS-Induced Colitis	74:92	DSS-Induced Colitis in Adulthood	74:105	Maternal High Fat Diet Alters Gut Microbiota of Offspring and Exacerbates DSS-Induced Colitis in Adulthood.					
30483266	1	80	theme	high	153:156	arg1	diet					162:165	high fat diet	153:165	high fat diet	153:165	Background: Accumulating evidence shows that high fat diet is closely associated with inflammatory bowel disease.					
30483266	4	81	theme	barrier	596:602	arg1	function					604:611	mucosal barrier function	588:611	mucosal barrier function	588:611	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	11	82	theme	control	1649:1655	arg1	diet					1657:1660	control diet	1649:1660	control diet	1649:1660	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	9	83	theme	cytokines	1384:1392	arg1	production					1357:1366	increased production	1347:1366	increased production of inflammatory cytokines	1347:1392	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	2	84	theme	diet	290:293	arg1	effects					235:241	effects	235:241	effects	235:241	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	2	84	theme	diet	290:293	arg1	mechanisms					258:267	underlying mechanisms	247:267	underlying mechanisms	247:267	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	4	85	theme	old	661:663	arg1	offspring					665:673	3-week old offspring	654:673	3-week old offspring	654:673	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	11	86	theme	offspring	1708:1716	arg1	different					1742:1750	different	1742:1750	different	1742:1750	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	11	86	theme	offspring	1708:1716	arg1	composition					1693:1703	the microbiota composition	1678:1703	the microbiota composition of offspring in MHFD group	1678:1730	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	9	87	theme	low-grade	1404:1412	arg1	inflammation					1414:1425	low-grade inflammation	1404:1425	low-grade inflammation	1404:1425	Although H&E staining showed no obvious microscopic inflammation in both groups of 3-week old offspring, increased production of inflammatory cytokines indicated low-grade inflammation was induced in MHFD group.					
30483266	10	88	theme	offspring	1497:1505	arg1	analysis					1470:1477	fecal analysis	1464:1477	fecal analysis of the 3-week old offspring	1464:1505	Moreover, fecal analysis of the 3-week old offspring indicated that the microbiota compositions and diversity were significantly changed in MHFD group.					
30483266	0	89	theme	High	9:12	arg1	Diet					18:21	Maternal High Fat Diet	0:21	Maternal High Fat Diet	0:21	Maternal High Fat Diet Alters Gut Microbiota of Offspring and Exacerbates DSS-Induced Colitis in Adulthood.					
30483266	4	90	theme	mucosal	630:636	arg1	inflammation					638:649	mucosal inflammation	630:649	mucosal inflammation	630:649	The intestinal development, mucosal barrier function, microbiota, and mucosal inflammation of 3-week old offspring were assessed.					
30483266	10	91	theme	3-week	1486:1491	arg1	offspring					1497:1505	the 3-week old offspring	1482:1505	the 3-week old offspring	1482:1505	Moreover, fecal analysis of the 3-week old offspring indicated that the microbiota compositions and diversity were significantly changed in MHFD group.					
30483266	2	92	from	effects	235:241	arg1	susceptibility					309:322	the susceptibility	305:322	the susceptibility of offspring to colitis in adulthood	305:359	However, the effects and underlying mechanisms of maternal high fat diet (MHFD) on the susceptibility of offspring to colitis in adulthood lacks confirmation.					
30483266	3	93	dep	diet	476:479	arg1	fat					488:490	E% fat	485:490	control diet [10 E% fat	468:490	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	10	94	theme	microbiota	1526:1535	arg1	compositions					1537:1548	the microbiota compositions	1522:1548	the microbiota compositions	1522:1548	Moreover, fecal analysis of the 3-week old offspring indicated that the microbiota compositions and diversity were significantly changed in MHFD group.					
30483266	0	95	theme	Gut	30:32	arg1	Microbiota					34:43	Gut Microbiota	30:43	Gut Microbiota of Offspring	30:56	Maternal High Fat Diet Alters Gut Microbiota of Offspring and Exacerbates DSS-Induced Colitis in Adulthood.					
30483266	3	96	theme	pregnant	398:405	arg1	mice					407:410	C57BL/6 pregnant mice	390:410	C57BL/6 pregnant mice	390:410	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	8	97	theme	3-week	1220:1225	arg1	offspring					1231:1239	3-week old offspring	1220:1239	3-week old offspring	1220:1239	Compared with MCD group, MHFD significantly inhibited intestinal development and disrupted barrier function in 3-week old offspring.					
30483266	7	98	located	found	1034:1038	arg2	differences					1017:1027	no differences	1014:1027	no differences	1014:1027	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	7	98	located	found	1034:1038	arg1	weight					1052:1057	the body weight	1043:1057	the body weight between two groups at other measured time points	1043:1106	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	3	99	theme	high	432:435	arg1	fat					437:439	a high fat	430:439	a high fat (60 E% fat, MHFD group)	430:463	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	13	100	theme	DSS-induced	2232:2242	arg1	colitis					2244:2250	DSS-induced colitis	2232:2250	DSS-induced colitis in adulthood	2232:2263	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	7	101	theme	time	1096:1099	arg1	points					1101:1106	other measured time points	1081:1106	other measured time points	1081:1106	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	3	102	theme	MHFD	453:456	arg1	fat					448:450	60 E% fat	442:450	60 E% fat	442:450	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	102	theme	MHFD	453:456	arg1	group					458:462	MHFD group	453:462	MHFD group	453:462	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	11	103	theme	MHFD	1721:1724	arg1	group					1726:1730	MHFD group	1721:1730	MHFD group	1721:1730	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	13	104	theme	barrier	2198:2204	arg1	disruption					2165:2174	the disruption	2161:2174	the disruption of intestinal mucosal barrier in offspring	2161:2217	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	7	105	theme	other	1081:1085	arg1	points					1101:1106	other measured time points	1081:1106	other measured time points	1081:1106	Results: The offspring in MHFD group were significantly heavier than those in MCD group only at 2-4 weeks of age, while no differences were found in the body weight between two groups at other measured time points.					
30483266	3	106	theme	E	445:445	arg1	fat					448:450	60 E% fat	442:450	60 E% fat	442:450	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	106	theme	E	445:445	arg1	group					458:462	MHFD group	453:462	MHFD group	453:462	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	107	dep	fat	437:439	arg1	fat					448:450	60 E% fat	442:450	60 E% fat	442:450	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	3	107	dep	fat	437:439	arg1	group					458:462	MHFD group	453:462	MHFD group	453:462	Methods: C57BL/6 pregnant mice were given either a high fat (60 E% fat, MHFD group) or control diet [10 E% fat, maternal control diet (MCD) group] during gestation and lactation.					
30483266	11	108	theme	MCD	1765:1767	arg1	group					1769:1773	MCD group	1765:1773	MCD group	1765:1773	Interestingly after 5 weeks consumption of control diet in both groups, the microbiota composition of offspring in MHFD group was still different from that in MCD group, although the bacterial diversity was partly recovered at 8 weeks of age.					
30483266	12	109	theme	cytokine	2006:2013	arg1	production					1976:1985	the production	1972:1985	the production of proinflammatory cytokine	1972:2013	Finally, after DSS treatment in 8-week old offspring, MHFD significantly exacerbated the severity of colitis and increased the production of proinflammatory cytokine.					
30483266	13	110	from	disruption	2165:2174	arg1	offspring					2209:2217	offspring	2209:2217	offspring	2209:2217	Conclusions: Our data reveal that MHFD in early life can inhibit intestinal development, induce dysbiosis and low-grade inflammation and lead to the disruption of intestinal mucosal barrier in offspring, and enhance DSS-induced colitis in adulthood.					
30483266	10	111	theme	MHFD	1594:1597	arg1	group					1599:1603	MHFD group	1594:1603	MHFD group	1594:1603	Moreover, fecal analysis of the 3-week old offspring indicated that the microbiota compositions and diversity were significantly changed in MHFD group.					
29845906	6	0	theme	zonula	1075:1080	arg1	occludens-1					1082:1092	zonula occludens-1	1075:1092	zonula occludens-1	1075:1092	By contrast, TGS down-regulated the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade.					
29845906	8	1	theme	caecal	1632:1637	arg1	mucosa					1639:1644	the caecal mucosa	1628:1644	the caecal mucosa	1628:1644	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	1	2	theme	intestinal	265:274	arg1	inflammation					276:287	intestinal inflammation	265:287	intestinal inflammation	265:287	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	7	3	from	signature	1239:1247	arg1	signalling					1286:1295	ileal, caecal and colonic mucosal signalling	1252:1295	ileal, caecal and colonic mucosal signalling	1252:1295	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	6	4	theme	NF-κB	1155:1159	arg1	cascade					1189:1195	NF-κB pro-inflammatory signalling cascade	1155:1195	NF-κB pro-inflammatory signalling cascade	1155:1195	By contrast, TGS down-regulated the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade.					
29845906	3	5	theme	mucosal	551:557	arg1	defence					559:565	the mucosal defence	547:565	the mucosal defence in jejunum, ileum, caecum and colon of pigs	547:609	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	4	6	theme	alterations	650:660	arg1	interactions					622:633	interactions	622:633	interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses	622:729	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	1	7	link	activity-derived	233:248	arg1	attenuation					250:260	microbial activity-derived attenuation	223:260	microbial activity-derived attenuation of intestinal inflammation	223:287	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	4	8	from	alterations	650:660	arg1	composition					691:701	composition	691:701	composition	691:701	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	4	8	from	alterations	650:660	arg1	metabolites					675:685	bacterial metabolites	665:685	bacterial metabolites	665:685	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	6	9	theme	toll-like	1130:1138	arg1	receptor					1140:1147	toll-like receptor 4	1130:1149	toll-like receptor 4	1130:1149	By contrast, TGS down-regulated the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade.					
29845906	4	10	theme	bacterial	665:673	arg1	metabolites					675:685	bacterial metabolites	665:685	bacterial metabolites	665:685	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	2	11	theme	anti-inflammatory	321:337	arg1	properties					339:348	anti-inflammatory properties	321:348	anti-inflammatory properties of RS of type 4	321:364	However, little is known about anti-inflammatory properties of RS of type 4.					
29845906	8	12	theme	microbial	1719:1727	arg1	action					1729:1734	microbial action	1719:1734	microbial action in the large intestine	1719:1757	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	7	13	theme	propionic	1372:1380	arg1	acid					1382:1385	propionic acid	1372:1385	propionic acid	1372:1385	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	0	14	theme	pig	137:139	arg1	model					141:145	a pig model	135:145	a pig model	135:145	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	4	15	theme	mucosal	713:719	arg1	responses					721:729	host mucosal responses	708:729	host mucosal responses	708:729	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	8	16	theme	large	1743:1747	arg1	intestine					1749:1757	the large intestine	1739:1757	the large intestine	1739:1757	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	6	17	theme	signalling	1178:1187	arg1	cascade					1189:1195	NF-κB pro-inflammatory signalling cascade	1155:1195	NF-κB pro-inflammatory signalling cascade	1155:1195	By contrast, TGS down-regulated the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade.					
29845906	5	18	dep	d.	935:936	arg1	modify					965:970	modify	965:970	did not modify jejunal barrier function and gene expression	957:1015	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	0	19	theme	innate	77:82	arg1	genes					100:104	mucosal innate immune response genes	69:104	mucosal innate immune response genes	69:104	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	5	20	theme	age	891:893	arg1	months					881:886	4 months	879:886	n 8/diet; 4 months of age	869:893	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	0	21	theme	response	91:98	arg1	genes					100:104	mucosal innate immune response genes	69:104	mucosal innate immune response genes	69:104	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	1	22	theme	resistant	160:168	arg1	RS					178:179	RS	178:179	RS	178:179	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	1	22	theme	resistant	160:168	arg1	starch					170:175	resistant starch	160:175	resistant starch (RS) consumption	160:192	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	7	23	from	genes	1430:1434	arg1	caecum					1446:1451	caecum	1446:1451	caecum	1446:1451	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	7	23	from	genes	1430:1434	arg1	ileum					1439:1443	ileum	1439:1443	ileum	1439:1443	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	7	23	from	genes	1430:1434	arg1	colon					1457:1461	colon	1457:1461	colon	1457:1461	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	4	24	theme	squares	772:778	arg1	regression					780:789	sparse partial least squares regression and relevance network analysis	751:820	regression	780:789	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	3	25	gly	transglycosylated	402:418	arg1	consumption					433:443	transglycosylated starch (TGS) consumption	402:443	transglycosylated starch (TGS) consumption	402:443	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	5	26	theme	TGS	953:955	arg1	Consumption					938:948	Consumption	938:948	Consumption of TGS	938:955	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	8	27	theme	present	1491:1497	arg1	findings					1499:1506	the present findings	1487:1506	the present findings	1487:1506	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	0	28	theme	large	113:117	arg1	intestine					119:127	the large intestine	109:127	the large intestine using a pig model	109:145	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	3	29	from	effects	391:397	arg1	expression					481:490	expression	481:490	expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs	481:609	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	29	from	effects	391:397	arg1	function					468:475	the jejunal barrier function	448:475	the jejunal barrier function	448:475	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	6	30	theme	caecal	1054:1059	arg1	expression					1061:1070	the caecal expression	1050:1070	the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade	1050:1195	By contrast, TGS down-regulated the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade.					
29845906	8	31	theme	anti-inflammatory	1524:1540	arg1	capabilities					1542:1553	certain anti-inflammatory capabilities	1516:1553	certain anti-inflammatory capabilities of TGS	1516:1560	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	4	32	theme	partial	758:764	arg1	squares					772:778	sparse partial least squares	751:778	sparse partial least squares	751:778	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	0	33	theme	transglycosylated	15:31	arg1	starch					33:38	transglycosylated starch	15:38	transglycosylated starch	15:38	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	7	34	theme	TGS-derived	1300:1310	arg1	changes					1312:1318	TGS-derived changes	1300:1318	TGS-derived changes	1300:1318	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	7	34	theme	TGS-derived	1300:1310	arg1	acid					1382:1385	propionic acid	1372:1385	propionic acid	1372:1385	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	3	35	theme	jejunal	452:458	arg1	function					468:475	the jejunal barrier function	448:475	the jejunal barrier function	448:475	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	5	36	theme	gene	1001:1004	arg1	expression					1006:1015	gene expression	1001:1015	gene expression	1001:1015	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	3	37	theme	transglycosylated	402:418	arg1	consumption					433:443	transglycosylated starch (TGS) consumption	402:443	transglycosylated starch (TGS) consumption	402:443	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	38	theme	genes	495:499	arg1	expression					481:490	expression	481:490	expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs	481:609	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	38	theme	genes	495:499	arg1	function					468:475	the jejunal barrier function	448:475	the jejunal barrier function	448:475	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	7	39	theme	colonic	1270:1276	arg1	signalling					1286:1295	ileal, caecal and colonic mucosal signalling	1252:1295	ileal, caecal and colonic mucosal signalling	1252:1295	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	6	40	theme	pro-inflammatory	1161:1176	arg1	cascade					1189:1195	NF-κB pro-inflammatory signalling cascade	1155:1195	NF-κB pro-inflammatory signalling cascade	1155:1195	By contrast, TGS down-regulated the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade.					
29845906	4	41	with	interactions	622:633	arg1	responses					721:729	host mucosal responses	708:729	host mucosal responses	708:729	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	4	42	theme	network	805:811	arg1	analysis					813:820	sparse partial least squares regression and relevance network analysis	751:820	analysis	813:820	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	2	43	theme	RS	353:354	arg1	properties					339:348	anti-inflammatory properties	321:348	anti-inflammatory properties of RS of type 4	321:364	However, little is known about anti-inflammatory properties of RS of type 4.					
29845906	5	44	theme	jejunal	972:978	arg1	function					988:995	jejunal barrier function	972:995	jejunal barrier function	972:995	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	7	45	from	changes	1312:1318	arg1	acids					1357:1361	fermentation acids	1344:1361	fermentation acids	1344:1361	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	7	45	from	changes	1312:1318	arg1	genera					1333:1338	bacterial genera	1323:1338	bacterial genera	1323:1338	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	7	46	theme	caecal	1259:1264	arg1	signalling					1286:1295	ileal, caecal and colonic mucosal signalling	1252:1295	ileal, caecal and colonic mucosal signalling	1252:1295	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	8	47	from	changes	1708:1714	arg1	action					1729:1734	microbial action	1719:1734	microbial action in the large intestine	1719:1757	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	1	48	theme	activity-derived	233:248	arg1	attenuation					250:260	microbial activity-derived attenuation	223:260	microbial activity-derived attenuation of intestinal inflammation	223:287	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	7	49	theme	pigs	1466:1469	arg1	caecum					1446:1451	caecum	1446:1451	caecum	1446:1451	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	7	49	theme	pigs	1466:1469	arg1	ileum					1439:1443	ileum	1439:1443	ileum	1439:1443	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	7	49	theme	pigs	1466:1469	arg1	colon					1457:1461	colon	1457:1461	colon	1457:1461	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	7	50	theme	ileal	1252:1256	arg1	signalling					1286:1295	ileal, caecal and colonic mucosal signalling	1252:1295	ileal, caecal and colonic mucosal signalling	1252:1295	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	8	51	theme	pathways	1616:1623	arg1	expression					1585:1594	the expression	1581:1594	the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine	1581:1757	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	7	52	theme	Relevance	1198:1206	arg1	networks					1208:1215	Relevance networks	1198:1215	Relevance networks	1198:1215	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	4	53	from	composition	691:701	arg1	interactions					622:633	interactions	622:633	interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses	622:729	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	3	54	from	inflammation	512:523	arg1	caecum					586:591	caecum	586:591	caecum	586:591	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	54	from	inflammation	512:523	arg1	ileum					579:583	ileum	579:583	ileum	579:583	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	54	from	inflammation	512:523	arg1	jejunum					570:576	jejunum	570:576	jejunum	570:576	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	54	from	inflammation	512:523	arg1	colon					597:601	colon	597:601	colon	597:601	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	4	55	from	metabolites	675:685	arg1	interactions					622:633	interactions	622:633	interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses	622:729	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	1	56	theme	inflammation	276:287	arg1	attenuation					250:260	microbial activity-derived attenuation	223:260	microbial activity-derived attenuation of intestinal inflammation	223:287	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	7	57	link	TGS-derived	1300:1310	arg1	changes					1312:1318	TGS-derived changes	1300:1318	TGS-derived changes	1300:1318	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	7	57	link	TGS-derived	1300:1310	arg1	acid					1382:1385	propionic acid	1372:1385	propionic acid	1372:1385	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	2	58	theme	type	359:362	arg1	RS					353:354	RS	353:354	RS of type 4	353:364	However, little is known about anti-inflammatory properties of RS of type 4.					
29845906	8	59	from	expression	1585:1594	arg1	mucosa					1639:1644	the caecal mucosa	1628:1644	the caecal mucosa	1628:1644	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	1	60	theme	starch	170:175	arg1	consumption					182:192	resistant starch (RS) consumption	160:192	resistant starch (RS) consumption	160:192	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	7	61	theme	expressed	1420:1428	arg1	genes					1430:1434	the differently expressed genes	1404:1434	the differently expressed genes in ileum, caecum and colon of pigs	1404:1469	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	4	62	theme	TGS-induced	638:648	arg1	alterations					650:660	TGS-induced alterations	638:660	TGS-induced alterations in bacterial metabolites and composition	638:701	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	8	63	from	action	1729:1734	arg1	intestine					1749:1757	the large intestine	1739:1757	the large intestine	1739:1757	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	4	64	from	interactions	622:633	arg1	composition					691:701	composition	691:701	composition	691:701	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	4	64	from	interactions	622:633	arg1	metabolites					675:685	bacterial metabolites	665:685	bacterial metabolites	665:685	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	1	65	theme	consumption	182:192	arg1	Benefits					148:155	Benefits	148:155	Benefits of resistant starch (RS) consumption on host physiology	148:211	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	6	66	theme	genes	1113:1117	arg1	expression					1061:1070	the caecal expression	1050:1070	the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade	1050:1195	By contrast, TGS down-regulated the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade.					
29845906	6	67	dep	receptor	1140:1147	arg1	the					1126:1128	the	1126:1128	the	1126:1128	By contrast, TGS down-regulated the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade.					
29845906	1	68	theme	host	197:200	arg1	physiology					202:211	host physiology	197:211	host physiology	197:211	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	4	69	theme	host	708:711	arg1	responses					721:729	host mucosal responses	708:729	host mucosal responses	708:729	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	3	70	from	defence	559:565	arg1	caecum					586:591	caecum	586:591	caecum	586:591	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	70	from	defence	559:565	arg1	ileum					579:583	ileum	579:583	ileum	579:583	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	70	from	defence	559:565	arg1	jejunum					570:576	jejunum	570:576	jejunum	570:576	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	70	from	defence	559:565	arg1	colon					597:601	colon	597:601	colon	597:601	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	71	theme	pigs	606:609	arg1	caecum					586:591	caecum	586:591	caecum	586:591	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	71	theme	pigs	606:609	arg1	ileum					579:583	ileum	579:583	ileum	579:583	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	71	theme	pigs	606:609	arg1	jejunum					570:576	jejunum	570:576	jejunum	570:576	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	71	theme	pigs	606:609	arg1	colon					597:601	colon	597:601	colon	597:601	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	5	72	theme	experimental	909:920	arg1	diets					922:926	the experimental diets	905:926	the experimental diets	905:926	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	8	73	theme	TGS-induced	1696:1706	arg1	changes					1708:1714	TGS-induced changes	1696:1714	TGS-induced changes in microbial action in the large intestine	1696:1757	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	0	74	theme	immune	84:89	arg1	genes					100:104	mucosal innate immune response genes	69:104	mucosal innate immune response genes	69:104	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	7	75	theme	fermentation	1344:1355	arg1	acids					1357:1361	fermentation acids	1344:1361	fermentation acids	1344:1361	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	3	76	from	function	534:541	arg1	caecum					586:591	caecum	586:591	caecum	586:591	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	76	from	function	534:541	arg1	ileum					579:583	ileum	579:583	ileum	579:583	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	76	from	function	534:541	arg1	jejunum					570:576	jejunum	570:576	jejunum	570:576	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	76	from	function	534:541	arg1	colon					597:601	colon	597:601	colon	597:601	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	0	77	theme	genes	100:104	arg1	expression					55:64	expression	55:64	expression of mucosal innate immune response genes in the large intestine using a pig model	55:145	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	4	78	theme	relevance	795:803	arg1	analysis					813:820	sparse partial least squares regression and relevance network analysis	751:820	analysis	813:820	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	4	79	theme	least	766:770	arg1	squares					772:778	sparse partial least squares	751:778	sparse partial least squares	751:778	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	1	80	from	Benefits	148:155	arg1	physiology					202:211	host physiology	197:211	host physiology	197:211	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	7	81	theme	bacterial	1323:1331	arg1	genera					1333:1338	bacterial genera	1323:1338	bacterial genera	1323:1338	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	0	82	from	expression	55:64	arg1	intestine					119:127	the large intestine	109:127	the large intestine using a pig model	109:145	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	5	83	theme	Intestinal	823:832	arg1	samples					834:840	Intestinal samples	823:840	Intestinal samples	823:840	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	3	84	theme	consumption	433:443	arg1	effects					391:397	the effects	387:397	the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs	387:609	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	0	85	theme	starch	33:38	arg1	Consumption					0:10	Consumption	0:10	Consumption of transglycosylated starch	0:38	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	8	86	theme	TGS	1558:1560	arg1	capabilities					1542:1553	certain anti-inflammatory capabilities	1516:1553	certain anti-inflammatory capabilities of TGS	1516:1560	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	4	87	theme	sparse	751:756	arg1	squares					772:778	sparse partial least squares	751:778	sparse partial least squares	751:778	Moreover, interactions of TGS-induced alterations in bacterial metabolites and composition with host mucosal responses were assessed using sparse partial least squares regression and relevance network analysis.					
29845906	7	88	theme	mucosal	1278:1284	arg1	signalling					1286:1295	ileal, caecal and colonic mucosal signalling	1252:1295	ileal, caecal and colonic mucosal signalling	1252:1295	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29845906	3	89	theme	barrier	460:466	arg1	function					468:475	the jejunal barrier function	448:475	the jejunal barrier function	448:475	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	0	90	theme	mucosal	69:75	arg1	genes					100:104	mucosal innate immune response genes	69:104	mucosal innate immune response genes	69:104	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	0	91	gly	transglycosylated	15:31	arg1	starch					33:38	transglycosylated starch	15:38	transglycosylated starch	15:38	Consumption of transglycosylated starch down-regulates expression of mucosal innate immune response genes in the large intestine using a pig model.					
29845906	8	92	theme	certain	1516:1522	arg1	capabilities					1542:1553	certain anti-inflammatory capabilities	1516:1553	certain anti-inflammatory capabilities of TGS	1516:1560	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	3	93	theme	starch	420:425	arg1	consumption					433:443	transglycosylated starch (TGS) consumption	402:443	transglycosylated starch (TGS) consumption	402:443	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	3	94	theme	related	501:507	arg1	genes					495:499	genes	495:499	genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs	495:609	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	8	95	theme	pro-inflammatory	1599:1614	arg1	pathways					1616:1623	pro-inflammatory pathways	1599:1623	pro-inflammatory pathways	1599:1623	In conclusion, the present findings suggest certain anti-inflammatory capabilities of TGS by down-regulating the expression of pro-inflammatory pathways in the caecal mucosa, which seems to be mediated, at least in part, by TGS-induced changes in microbial action in the large intestine.					
29845906	5	96	dep	n	869:869	arg1	8/diet					871:876	8/diet	871:876	n 8/diet; 4 months of age	869:893	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	5	96	dep	n	869:869	arg1	months					881:886	4 months	879:886	n 8/diet; 4 months of age	869:893	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	6	97	theme	occludens-1	1082:1092	arg1	expression					1061:1070	the caecal expression	1050:1070	the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade	1050:1195	By contrast, TGS down-regulated the caecal expression of zonula occludens-1 and mucin 2 and of genes within the toll-like receptor 4 and NF-κB pro-inflammatory signalling cascade.					
29845906	1	98	theme	microbial	223:231	arg1	attenuation					250:260	microbial activity-derived attenuation	223:260	microbial activity-derived attenuation of intestinal inflammation	223:287	Benefits of resistant starch (RS) consumption on host physiology encompass microbial activity-derived attenuation of intestinal inflammation.					
29845906	5	99	theme	barrier	980:986	arg1	function					988:995	jejunal barrier function	972:995	jejunal barrier function	972:995	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	5	100	dep	pigs	863:866	arg1	n					869:869	n	869:869	n 8/diet; 4 months of age	869:893	Intestinal samples were collected after pigs (n 8/diet; 4 months of age) were fed the experimental diets for 10 d. Consumption of TGS did not modify jejunal barrier function and gene expression.					
29845906	3	101	theme	barrier	526:532	arg1	function					534:541	barrier function	526:541	barrier function	526:541	This study compared the effects of transglycosylated starch (TGS) consumption on the jejunal barrier function and expression of genes related to inflammation, barrier function and the mucosal defence in jejunum, ileum, caecum and colon of pigs.					
29845906	7	102	theme	microbiome	1228:1237	arg1	signature					1239:1247	a microbiome signature	1226:1247	a microbiome signature on ileal, caecal and colonic mucosal signalling	1226:1295	Relevance networks revealed a microbiome signature on ileal, caecal and colonic mucosal signalling as TGS-derived changes in bacterial genera and fermentation acids, such as propionic acid, correlated with the differently expressed genes in ileum, caecum and colon of pigs.					
29635103	7	0	theme	chemical	980:987	arg1	compounds					989:997	well-defined chemical compounds	967:997	well-defined chemical compounds	967:997	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	1	theme	for	1075:1077	arg1	BAM					1090:1092	BAM	1090:1092	BAM	1090:1092	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	1	theme	for	1075:1077	arg1	membranes					1079:1087	biocompatible anchor for membranes	1054:1087	biocompatible anchor for membranes (BAM)	1054:1093	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	10	2	theme	treatment	1570:1578	arg1	efficacy					1554:1561	the efficacy	1550:1561	the efficacy of our treatment in Panc02 murine model of aggressive pancreatic tumor	1550:1632	We tested the efficacy of our treatment in Panc02 murine model of aggressive pancreatic tumor as well.					
29635103	8	3	dep	effect	1357:1362	arg1	3					1328:1328	3	1328:1328	3	1328:1328	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	12	4	theme	cancer	1870:1875	arg1	approach					1846:1853	herein presented immunotherapeutic approach	1811:1853	herein presented immunotherapeutic approach	1811:1853	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	12	4	theme	cancer	1870:1875	arg1	strategy					1887:1894	a promising cancer treatment strategy	1858:1894	a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases	1858:1968	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	8	5	from	eradication	1197:1207	arg1	mice					1258:1261	mice	1258:1261	mice	1258:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	8	5	from	eradication	1197:1207	arg1	%					1253:1253	83%	1251:1253	83% of mice	1251:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	7	6	theme	biocompatible	1054:1066	arg1	BAM					1090:1092	BAM	1090:1092	BAM	1090:1092	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	6	theme	biocompatible	1054:1066	arg1	membranes					1079:1087	biocompatible anchor for membranes	1054:1087	biocompatible anchor for membranes (BAM)	1054:1093	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	10	7	theme	aggressive	1606:1615	arg1	tumor					1628:1632	aggressive pancreatic tumor	1606:1632	aggressive pancreatic tumor	1606:1632	We tested the efficacy of our treatment in Panc02 murine model of aggressive pancreatic tumor as well.					
29635103	8	8	from	effect	1357:1362	arg1	mice					1258:1261	mice	1258:1261	mice	1258:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	8	8	from	effect	1357:1362	arg1	%					1253:1253	83%	1251:1253	83% of mice	1251:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	7	9	theme	tumor	1032:1036	arg1	surface					1043:1049	tumor cell surface	1032:1049	tumor cell surface	1032:1049	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	5	10	theme	melanoma	814:821	arg1	treatment					801:809	successful treatment	790:809	successful treatment of melanoma	790:821	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	11	11	theme	Simultaneous	1643:1654	arg1	application					1656:1666	Simultaneous application	1643:1666	Simultaneous application of agonistic anti-CD40 antibody	1643:1698	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	10	12	theme	tumor	1628:1632	arg1	model					1597:1601	Panc02 murine model	1583:1601	Panc02 murine model of aggressive pancreatic tumor	1583:1632	We tested the efficacy of our treatment in Panc02 murine model of aggressive pancreatic tumor as well.					
29635103	8	13	from	acquisition	1268:1278	arg1	mice					1258:1261	mice	1258:1261	mice	1258:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	8	13	from	acquisition	1268:1278	arg1	%					1253:1253	83%	1251:1253	83% of mice	1251:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	12	14	theme	presented	1818:1826	arg1	approach					1846:1853	herein presented immunotherapeutic approach	1811:1853	herein presented immunotherapeutic approach	1811:1853	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	12	14	theme	presented	1818:1826	arg1	strategy					1887:1894	a promising cancer treatment strategy	1858:1894	a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases	1858:1968	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	3	15	theme	immune	299:304	arg1	activation					311:320	immune cell activation	299:320	immune cell activation	299:320	The use of microorganisms or their parts for immune cell activation, referred to as Pathogen-Associated Molecular Patterns (PAMPs), represents highly promising concept.					
29635103	11	16	theme	anti-CD40	1681:1689	arg1	antibody					1691:1698	agonistic anti-CD40 antibody	1671:1698	agonistic anti-CD40 antibody	1671:1698	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	5	17	theme	ligand	674:679	arg1	combination					635:645	the combination	631:645	the combination of phagocytosis activating ligand (mannan)	631:688	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	17	theme	ligand	674:679	arg1	mixture					694:700	mixture	694:700	mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma	694:821	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	12	18	with	strategy	1887:1894	arg1	ability					1905:1911	the ability to eradicate not only primary tumors but also metastases	1901:1968	the ability to eradicate not only primary tumors but also metastases	1901:1968	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	2	19	theme	different	208:216	arg1	immunotherapies					218:232	numerous different immunotherapies	199:232	numerous different immunotherapies	199:232	Up to date, the efficacy of numerous different immunotherapies has been evaluated.					
29635103	3	20	theme	parts	289:293	arg1	use					258:260	The use	254:260	The use	254:260	The use of microorganisms or their parts for immune cell activation, referred to as Pathogen-Associated Molecular Patterns (PAMPs), represents highly promising concept.					
29635103	7	21	theme	well-defined	967:978	arg1	compounds					989:997	well-defined chemical compounds	967:997	well-defined chemical compounds	967:997	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	1	22	theme	fundamental	129:139	arg1	approach					141:148	a fundamental approach	127:148	a fundamental approach in cancer treatment	127:168	Immunotherapy emerges as a fundamental approach in cancer treatment.					
29635103	4	23	theme	therapeutic	427:437	arg1	effect					439:444	The therapeutic effect	423:444	The therapeutic effect of PAMPs	423:453	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	0	24	theme	agonists	59:66	arg1	combination					40:50	combination	40:50	combination of TLR agonists with stimulation of phagocytosis	40:99	Effective cancer immunotherapy based on combination of TLR agonists with stimulation of phagocytosis.					
29635103	7	25	theme	TLR	1099:1101	arg1	acid					1152:1155	lipoteichoic acid	1139:1155	lipoteichoic acid (LTA)	1139:1161	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	25	theme	TLR	1099:1101	arg1	agonists					1103:1110	TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA)	1099:1161	TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA)	1099:1161	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	25	theme	TLR	1099:1101	arg1	poly					1124:1127	poly	1124:1127	poly(I:C)	1124:1132	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	25	theme	TLR	1099:1101	arg1	resiquimod					1112:1121	resiquimod	1112:1121	resiquimod	1112:1121	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	9	26	theme	further	1371:1377	arg1	investigation					1379:1391	further investigation	1371:1391	further investigation of mechanisms, underlying anti-tumor responses	1371:1438	After further investigation of mechanisms, underlying anti-tumor responses, we concluded that both innate and adaptive immunity are activated and involved in these processes.					
29635103	8	27	theme	potential	1331:1339	arg1	effect					1357:1362	(3) potential anti-metastatic effect	1327:1362	(3) potential anti-metastatic effect	1327:1362	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	12	28	theme	primary	1935:1941	arg1	tumors					1943:1948	primary tumors	1935:1948	not only primary tumors but also metastases	1926:1968	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	5	29	theme	phagocytosis	650:661	arg1	mannan					682:687	mannan	682:687	mannan	682:687	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	29	theme	phagocytosis	650:661	arg1	ligand					674:679	phagocytosis activating ligand	650:679	phagocytosis activating ligand (mannan)	650:688	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	6	30	theme	used	924:927	arg1	mixture					929:935	previously used mixture	913:935	previously used mixture	913:935	In the present study, we optimized the composition and timing of previously used mixture.					
29635103	3	31	theme	Molecular	358:366	arg1	PAMPs					378:382	PAMPs	378:382	PAMPs	378:382	The use of microorganisms or their parts for immune cell activation, referred to as Pathogen-Associated Molecular Patterns (PAMPs), represents highly promising concept.					
29635103	3	31	theme	Molecular	358:366	arg1	Patterns					368:375	Pathogen-Associated Molecular Patterns	338:375	Pathogen-Associated Molecular Patterns (PAMPs)	338:383	The use of microorganisms or their parts for immune cell activation, referred to as Pathogen-Associated Molecular Patterns (PAMPs), represents highly promising concept.					
29635103	4	32	theme	different	507:515	arg1	types					517:521	different types	507:521	different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands	507:608	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	7	33	dep	agonists	1103:1110	arg1	acid					1152:1155	lipoteichoic acid	1139:1155	lipoteichoic acid (LTA)	1139:1161	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	33	dep	agonists	1103:1110	arg1	agonists					1103:1110	TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA)	1099:1161	TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA)	1099:1161	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	33	dep	agonists	1103:1110	arg1	resiquimod					1112:1121	resiquimod	1112:1121	resiquimod	1112:1121	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	33	dep	agonists	1103:1110	arg1	C					1131:1131	I:C	1129:1131	I:C	1129:1131	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	33	dep	agonists	1103:1110	arg1	LTA					1158:1160	LTA	1158:1160	LTA	1158:1160	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	33	dep	agonists	1103:1110	arg1	poly					1124:1127	poly	1124:1127	poly(I:C)	1124:1132	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	8	34	theme	stage	1221:1225	arg1	melanoma					1239:1246	advanced stage progressive melanoma	1212:1246	advanced stage progressive melanoma in 83% of mice	1212:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	0	35	theme	phagocytosis	88:99	arg1	stimulation					73:83	stimulation	73:83	stimulation of phagocytosis	73:99	Effective cancer immunotherapy based on combination of TLR agonists with stimulation of phagocytosis.					
29635103	8	36	theme	melanoma	1239:1246	arg1	eradication					1197:1207	(1) eradication	1193:1207	(1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect	1193:1362	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	9	37	theme	anti-tumor	1419:1428	arg1	responses					1430:1438	anti-tumor responses	1419:1438	anti-tumor responses	1419:1438	After further investigation of mechanisms, underlying anti-tumor responses, we concluded that both innate and adaptive immunity are activated and involved in these processes.					
29635103	0	38	theme	Effective	0:8	arg1	immunotherapy					17:29	Effective cancer immunotherapy	0:29	Effective cancer immunotherapy	0:29	Effective cancer immunotherapy based on combination of TLR agonists with stimulation of phagocytosis.					
29635103	4	39	theme	Toll	540:543	arg1	TLR					560:562	TLR	560:562	TLR	560:562	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	4	39	theme	Toll	540:543	arg1	receptor					550:557	Toll like receptor	540:557	Toll like receptor (TLR) agonists	540:572	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	5	40	theme	inactivated	750:760	arg1	resiquimod					719:728	resiquimod	719:728	resiquimod (R-848)	719:736	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	40	theme	inactivated	750:760	arg1	monocytogenes					771:783	inactivated Listeria monocytogenes	750:783	inactivated Listeria monocytogenes	750:783	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	11	41	dep	%	1759:1759	arg1	recovery					1761:1768	recovery	1761:1768	80% recovery	1757:1768	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	3	42	theme	promising	404:412	arg1	concept					414:420	highly promising concept	397:420	highly promising concept	397:420	The use of microorganisms or their parts for immune cell activation, referred to as Pathogen-Associated Molecular Patterns (PAMPs), represents highly promising concept.					
29635103	4	43	theme	receptor	550:557	arg1	agonists					565:572	Toll like receptor (TLR) agonists	540:572	Toll like receptor (TLR) agonists	540:572	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	8	44	dep	eradication	1197:1207	arg1	1					1194:1194	1	1194:1194	1	1194:1194	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	11	45	theme	effective	1725:1733	arg1	response					1747:1754	effective therapeutic response	1725:1754	effective therapeutic response	1725:1754	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	8	46	theme	acquisition	1268:1278	arg1	eradication					1197:1207	(1) eradication	1193:1207	(1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect	1193:1362	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	5	47	theme	murine	826:831	arg1	model					841:845	murine B16-F10 model	826:845	murine B16-F10 model	826:845	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	4	48	theme	phagocytosis	578:589	arg1	ligands					602:608	phagocytosis activating ligands	578:608	phagocytosis activating ligands	578:608	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	8	49	theme	resistance	1283:1292	arg1	effect					1357:1362	(3) potential anti-metastatic effect	1327:1362	(3) potential anti-metastatic effect	1327:1362	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	8	49	theme	resistance	1283:1292	arg1	acquisition					1268:1278	(2) acquisition	1264:1278	(2) acquisition of resistance to tumor re-transplantation	1264:1320	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	8	49	theme	resistance	1283:1292	arg1	melanoma					1239:1246	advanced stage progressive melanoma	1212:1246	advanced stage progressive melanoma in 83% of mice	1212:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	10	50	theme	murine	1590:1595	arg1	model					1597:1601	Panc02 murine model	1583:1601	Panc02 murine model of aggressive pancreatic tumor	1583:1632	We tested the efficacy of our treatment in Panc02 murine model of aggressive pancreatic tumor as well.					
29635103	0	51	theme	TLR	55:57	arg1	agonists					59:66	TLR agonists	55:66	TLR agonists	55:66	Effective cancer immunotherapy based on combination of TLR agonists with stimulation of phagocytosis.					
29635103	6	52	theme	present	855:861	arg1	study					863:867	the present study	851:867	the present study	851:867	In the present study, we optimized the composition and timing of previously used mixture.					
29635103	7	53	theme	anchor	1068:1073	arg1	BAM					1090:1092	BAM	1090:1092	BAM	1090:1092	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	53	theme	anchor	1068:1073	arg1	membranes					1079:1087	biocompatible anchor for membranes	1054:1087	biocompatible anchor for membranes (BAM)	1054:1093	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	8	54	from	%	1253:1253	arg1	eradication					1197:1207	(1) eradication	1193:1207	(1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect	1193:1362	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	10	55	from	efficacy	1554:1561	arg1	model					1597:1601	Panc02 murine model	1583:1601	Panc02 murine model of aggressive pancreatic tumor	1583:1632	We tested the efficacy of our treatment in Panc02 murine model of aggressive pancreatic tumor as well.					
29635103	7	56	theme	lipoteichoic	1139:1150	arg1	acid					1152:1155	lipoteichoic acid	1139:1155	lipoteichoic acid (LTA)	1139:1161	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	56	theme	lipoteichoic	1139:1150	arg1	agonists					1103:1110	TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA)	1099:1161	TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA)	1099:1161	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	56	theme	lipoteichoic	1139:1150	arg1	LTA					1158:1160	LTA	1158:1160	LTA	1158:1160	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	10	57	theme	pancreatic	1617:1626	arg1	tumor					1628:1632	aggressive pancreatic tumor	1606:1632	aggressive pancreatic tumor	1606:1632	We tested the efficacy of our treatment in Panc02 murine model of aggressive pancreatic tumor as well.					
29635103	12	58	theme	promising	1860:1868	arg1	approach					1846:1853	herein presented immunotherapeutic approach	1811:1853	herein presented immunotherapeutic approach	1811:1853	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	12	58	theme	promising	1860:1868	arg1	strategy					1887:1894	a promising cancer treatment strategy	1858:1894	a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases	1858:1968	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	11	59	from	necessary	1704:1712	arg1	model					1779:1783	this model	1774:1783	this model	1774:1783	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	7	60	theme	cell	1038:1041	arg1	surface					1043:1049	tumor cell surface	1032:1049	tumor cell surface	1032:1049	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	5	61	theme	successful	790:799	arg1	treatment					801:809	successful treatment	790:809	successful treatment of melanoma	790:821	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	12	62	theme	treatment	1877:1885	arg1	approach					1846:1853	herein presented immunotherapeutic approach	1811:1853	herein presented immunotherapeutic approach	1811:1853	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	12	62	theme	treatment	1877:1885	arg1	strategy					1887:1894	a promising cancer treatment strategy	1858:1894	a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases	1858:1968	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	12	63	theme	immunotherapeutic	1828:1844	arg1	approach					1846:1853	herein presented immunotherapeutic approach	1811:1853	herein presented immunotherapeutic approach	1811:1853	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	12	63	theme	immunotherapeutic	1828:1844	arg1	strategy					1887:1894	a promising cancer treatment strategy	1858:1894	a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases	1858:1968	Our results suggest that herein presented immunotherapeutic approach is a promising cancer treatment strategy with the ability to eradicate not only primary tumors but also metastases.					
29635103	11	64	theme	agonistic	1671:1679	arg1	antibody					1691:1698	agonistic anti-CD40 antibody	1671:1698	agonistic anti-CD40 antibody	1671:1698	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	7	65	theme	mannan	1012:1017	arg1	anchoring					1019:1027	mannan anchoring	1012:1027	mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA)	1012:1161	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	5	66	theme	Listeria	762:769	arg1	resiquimod					719:728	resiquimod	719:728	resiquimod (R-848)	719:736	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	66	theme	Listeria	762:769	arg1	monocytogenes					771:783	inactivated Listeria monocytogenes	750:783	inactivated Listeria monocytogenes	750:783	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	4	67	theme	PAMPs	449:453	arg1	effect					439:444	The therapeutic effect	423:444	The therapeutic effect of PAMPs	423:453	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	3	68	theme	microorganisms	265:278	arg1	use					258:260	The use	254:260	The use	254:260	The use of microorganisms or their parts for immune cell activation, referred to as Pathogen-Associated Molecular Patterns (PAMPs), represents highly promising concept.					
29635103	5	69	theme	agonists	709:716	arg1	combination					635:645	the combination	631:645	the combination of phagocytosis activating ligand (mannan)	631:688	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	69	theme	agonists	709:716	arg1	mixture					694:700	mixture	694:700	mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma	694:821	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	11	70	from	model	1779:1783	arg1	necessary					1704:1712	necessary	1704:1712	necessary	1704:1712	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	5	71	theme	activating	663:672	arg1	mannan					682:687	mannan	682:687	mannan	682:687	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	71	theme	activating	663:672	arg1	ligand					674:679	phagocytosis activating ligand	650:679	phagocytosis activating ligand (mannan)	650:688	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	2	72	theme	immunotherapies	218:232	arg1	efficacy					187:194	the efficacy	183:194	the efficacy of numerous different immunotherapies	183:232	Up to date, the efficacy of numerous different immunotherapies has been evaluated.					
29635103	8	73	theme	effect	1357:1362	arg1	eradication					1197:1207	(1) eradication	1193:1207	(1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect	1193:1362	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	3	74	theme	cell	306:309	arg1	activation					311:320	immune cell activation	299:320	immune cell activation	299:320	The use of microorganisms or their parts for immune cell activation, referred to as Pathogen-Associated Molecular Patterns (PAMPs), represents highly promising concept.					
29635103	6	75	dep	composition	887:897	arg1	the					883:885	the	883:885	the	883:885	In the present study, we optimized the composition and timing of previously used mixture.					
29635103	11	76	theme	antibody	1691:1698	arg1	application					1656:1666	Simultaneous application	1643:1666	Simultaneous application of agonistic anti-CD40 antibody	1643:1698	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	7	77	theme	Therapeutic	938:948	arg1	mixture					950:956	Therapeutic mixture	938:956	Therapeutic mixture based on well-defined chemical compounds	938:997	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	8	78	dep	acquisition	1268:1278	arg1	2					1265:1265	2	1265:1265	2	1265:1265	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	5	79	dep	agonists	709:716	arg1	R-848					731:735	R-848	731:735	R-848	731:735	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	79	dep	agonists	709:716	arg1	poly					739:742	poly	739:742	poly(I:C)	739:747	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	79	dep	agonists	709:716	arg1	resiquimod					719:728	resiquimod	719:728	resiquimod (R-848)	719:736	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	79	dep	agonists	709:716	arg1	monocytogenes					771:783	inactivated Listeria monocytogenes	750:783	inactivated Listeria monocytogenes	750:783	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	8	80	theme	anti-metastatic	1341:1355	arg1	effect					1357:1362	(3) potential anti-metastatic effect	1327:1362	(3) potential anti-metastatic effect	1327:1362	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	7	81	theme	I	1129:1129	arg1	C					1131:1131	I:C	1129:1131	I:C	1129:1131	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	7	81	theme	I	1129:1129	arg1	poly					1124:1127	poly	1124:1127	poly(I:C)	1124:1132	Therapeutic mixture based on well-defined chemical compounds consisted of mannan anchoring to tumor cell surface by biocompatible anchor for membranes (BAM) and TLR agonists resiquimod, poly(I:C), and lipoteichoic acid (LTA).					
29635103	5	82	theme	B16-F10	833:839	arg1	model					841:845	murine B16-F10 model	826:845	murine B16-F10 model	826:845	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	6	83	theme	mixture	929:935	arg1	composition					887:897	composition	887:897	composition	887:897	In the present study, we optimized the composition and timing of previously used mixture.					
29635103	6	83	theme	mixture	929:935	arg1	timing					903:908	timing	903:908	timing	903:908	In the present study, we optimized the composition and timing of previously used mixture.					
29635103	4	84	theme	types	517:521	arg1	combination					492:502	suitable combination	483:502	suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands	483:608	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	8	85	theme	tumor	1297:1301	arg1	re-transplantation					1303:1320	tumor re-transplantation	1297:1320	tumor re-transplantation	1297:1320	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	5	86	theme	TLR	705:707	arg1	agonists					709:716	TLR agonists	705:716	TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma	705:821	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	9	87	theme	mechanisms	1396:1405	arg1	investigation					1379:1391	further investigation	1371:1391	further investigation of mechanisms, underlying anti-tumor responses	1371:1438	After further investigation of mechanisms, underlying anti-tumor responses, we concluded that both innate and adaptive immunity are activated and involved in these processes.					
29635103	8	88	theme	advanced	1212:1219	arg1	melanoma					1239:1246	advanced stage progressive melanoma	1212:1246	advanced stage progressive melanoma in 83% of mice	1212:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	3	89	theme	Pathogen-Associated	338:356	arg1	PAMPs					378:382	PAMPs	378:382	PAMPs	378:382	The use of microorganisms or their parts for immune cell activation, referred to as Pathogen-Associated Molecular Patterns (PAMPs), represents highly promising concept.					
29635103	3	89	theme	Pathogen-Associated	338:356	arg1	Patterns					368:375	Pathogen-Associated Molecular Patterns	338:375	Pathogen-Associated Molecular Patterns (PAMPs)	338:383	The use of microorganisms or their parts for immune cell activation, referred to as Pathogen-Associated Molecular Patterns (PAMPs), represents highly promising concept.					
29635103	4	90	theme	suitable	483:490	arg1	combination					492:502	suitable combination	483:502	suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands	483:608	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	8	91	theme	progressive	1227:1237	arg1	melanoma					1239:1246	advanced stage progressive melanoma	1212:1246	advanced stage progressive melanoma in 83% of mice	1212:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	0	92	theme	cancer	10:15	arg1	immunotherapy					17:29	Effective cancer immunotherapy	0:29	Effective cancer immunotherapy	0:29	Effective cancer immunotherapy based on combination of TLR agonists with stimulation of phagocytosis.					
29635103	11	93	dep	necessary	1704:1712	arg1	%					1759:1759	80%	1757:1759	80% recovery	1757:1768	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	1	94	from	approach	141:148	arg1	treatment					160:168	cancer treatment	153:168	cancer treatment	153:168	Immunotherapy emerges as a fundamental approach in cancer treatment.					
29635103	4	95	theme	like	545:548	arg1	TLR					560:562	TLR	560:562	TLR	560:562	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	4	95	theme	like	545:548	arg1	receptor					550:557	Toll like receptor	540:557	Toll like receptor (TLR) agonists	540:572	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	4	96	theme	PAMPs	526:530	arg1	types					517:521	different types	507:521	different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands	507:608	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	8	97	theme	mice	1258:1261	arg1	mice					1258:1261	mice	1258:1261	mice	1258:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	8	97	theme	mice	1258:1261	arg1	%					1253:1253	83%	1251:1253	83% of mice	1251:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	5	98	theme	I	744:744	arg1	poly					739:742	poly	739:742	poly(I:C)	739:747	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	5	98	theme	I	744:744	arg1	C					746:746	I:C	744:746	I:C	744:746	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	9	99	theme	innate	1464:1469	arg1	immunity					1484:1491	both innate and adaptive immunity	1459:1491	both innate and adaptive immunity	1459:1491	After further investigation of mechanisms, underlying anti-tumor responses, we concluded that both innate and adaptive immunity are activated and involved in these processes.					
29635103	2	100	theme	numerous	199:206	arg1	immunotherapies					218:232	numerous different immunotherapies	199:232	numerous different immunotherapies	199:232	Up to date, the efficacy of numerous different immunotherapies has been evaluated.					
29635103	1	101	theme	cancer	153:158	arg1	treatment					160:168	cancer treatment	153:168	cancer treatment	153:168	Immunotherapy emerges as a fundamental approach in cancer treatment.					
29635103	11	102	theme	therapeutic	1735:1745	arg1	response					1747:1754	effective therapeutic response	1725:1754	effective therapeutic response	1725:1754	Simultaneous application of agonistic anti-CD40 antibody was necessary to achieve effective therapeutic response (80% recovery) in this model.					
29635103	8	103	from	melanoma	1239:1246	arg1	mice					1258:1261	mice	1258:1261	mice	1258:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	8	103	from	melanoma	1239:1246	arg1	%					1253:1253	83%	1251:1253	83% of mice	1251:1261	The optimization resulted in (1) eradication of advanced stage progressive melanoma in 83% of mice, (2) acquisition of resistance to tumor re-transplantation, and (3) potential anti-metastatic effect.					
29635103	9	104	theme	adaptive	1475:1482	arg1	immunity					1484:1491	both innate and adaptive immunity	1459:1491	both innate and adaptive immunity	1459:1491	After further investigation of mechanisms, underlying anti-tumor responses, we concluded that both innate and adaptive immunity are activated and involved in these processes.					
29635103	4	105	theme	activating	591:600	arg1	ligands					602:608	phagocytosis activating ligands	578:608	phagocytosis activating ligands	578:608	The therapeutic effect of PAMPs can be further amplified by suitable combination of different types of PAMPs such as Toll like receptor (TLR) agonists and phagocytosis activating ligands.					
29635103	5	106	used	used	626:629	arg2	we					623:624	we	623:624	we	623:624	Previously, we used the combination of phagocytosis activating ligand (mannan) and mixture of TLR agonists (resiquimod (R-848), poly(I:C), inactivated Listeria monocytogenes) for successful treatment of melanoma in murine B16-F10 model.					
29635103	0	107	with	combination	40:50	arg1	stimulation					73:83	stimulation	73:83	stimulation of phagocytosis	73:99	Effective cancer immunotherapy based on combination of TLR agonists with stimulation of phagocytosis.					
29635103	10	108	theme	Panc02	1583:1588	arg1	model					1597:1601	Panc02 murine model	1583:1601	Panc02 murine model of aggressive pancreatic tumor	1583:1632	We tested the efficacy of our treatment in Panc02 murine model of aggressive pancreatic tumor as well.					
31029334	8	0	theme	particular	1052:1061	arg1	performance					1079:1089	The particular high antifungal performance	1048:1089	The particular high antifungal performance of CuO/C nanocomposite	1048:1112	The particular high antifungal performance of CuO/C nanocomposite was found against Aspergillus niger while compare to Aspergillus flavus fungal strain.					
31029334	1	1	theme	composite	146:154	arg1	synthesis					112:120	The synthesis	108:120	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent	108:187	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
31029334	1	2	theme	Response	235:242	arg1	Methodology					252:262	Response Surface Methodology	235:262	Response Surface Methodology	235:262	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
31029334	5	3	theme	Aspergillus	811:821	arg1	species					830:836	Aspergillus flavus species	811:836	Aspergillus flavus species	811:836	The antifungal activities of CuO/C nanocomposite were tested against Aspergillus niger and Aspergillus flavus species.					
31029334	6	4	theme	nanocomposite	860:872	arg1	1000 ppm					842:849	1000 ppm	842:849	1000 ppm of CuO/C nanocomposite	842:872	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	1	5	theme	Surface	244:250	arg1	Methodology					252:262	Response Surface Methodology	235:262	Response Surface Methodology	235:262	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
31029334	0	6	theme	antifungal	87:96	arg1	activity					98:105	its antifungal activity	83:105	its antifungal activity	83:105	CuO/C nanocomposite: Synthesis and optimization using sucrose as carbon source and its antifungal activity.					
31029334	7	7	theme	inhibition	954:963	arg1	mechanism					965:973	The fungal inhibition mechanism	943:973	The fungal inhibition mechanism of bioactive CuO/C nanocomposite	943:1006	The fungal inhibition mechanism of bioactive CuO/C nanocomposite was discussed in this research article.					
31029334	7	8	theme	CuO/C	988:992	arg1	nanocomposite					994:1006	bioactive CuO/C nanocomposite	978:1006	bioactive CuO/C nanocomposite	978:1006	The fungal inhibition mechanism of bioactive CuO/C nanocomposite was discussed in this research article.					
31029334	6	9	theme	CuO/C	854:858	arg1	nanocomposite					860:872	CuO/C nanocomposite	854:872	CuO/C nanocomposite	854:872	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	6	10	theme	%	918:918	arg1	hindrance					920:928	90% hindrance	916:928	90% hindrance	916:928	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	8	11	theme	high	1063:1066	arg1	performance					1079:1089	The particular high antifungal performance	1048:1089	The particular high antifungal performance of CuO/C nanocomposite	1048:1112	The particular high antifungal performance of CuO/C nanocomposite was found against Aspergillus niger while compare to Aspergillus flavus fungal strain.					
31029334	2	12	theme	sucrose	349:355	arg1	aggregation					334:344	aggregation	334:344	aggregation of sucrose	334:355	The synthesis process was done in a green environment which prevents aggregation of sucrose and promotes nanoparticles formation.					
31029334	3	13	theme	CuO/C	493:497	arg1	nanocomposite					499:511	CuO/C nanocomposite	493:511	CuO/C nanocomposite	493:511	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	3	13	theme	CuO/C	493:497	arg1	nanocomposites					477:490	copper oxide nanocomposites	464:490	copper oxide nanocomposites (CuO/C nanocomposite)	464:512	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	6	14	theme	90	916:917	arg1	%					918:918	%	918:918	%	918:918	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	2	15	theme	synthesis	269:277	arg1	process					279:285	The synthesis process	265:285	The synthesis process	265:285	The synthesis process was done in a green environment which prevents aggregation of sucrose and promotes nanoparticles formation.					
31029334	3	16	theme	carbon	441:446	arg1	source					448:453	a carbon source	439:453	a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm	439:544	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	3	16	theme	carbon	441:446	arg1	sucrose					428:434	sucrose	428:434	sucrose	428:434	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	7	17	theme	fungal	947:952	arg1	mechanism					965:973	The fungal inhibition mechanism	943:973	The fungal inhibition mechanism of bioactive CuO/C nanocomposite	943:1006	The fungal inhibition mechanism of bioactive CuO/C nanocomposite was discussed in this research article.					
31029334	3	18	theme	50 nm	540:544	arg1	size					532:535	the particle size	519:535	the particle size of 50 nm	519:544	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	6	19	from	restraint	889:897	arg1	flavus					905:910	A. flavus	902:910	A. flavus	902:910	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	6	19	from	restraint	889:897	arg1	hindrance					920:928	90% hindrance	916:928	90% hindrance	916:928	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	6	19	from	restraint	889:897	arg1	niger					936:940	niger	936:940	niger	936:940	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	8	20	theme	fungal	1186:1191	arg1	strain					1193:1198	Aspergillus flavus fungal strain	1167:1198	Aspergillus flavus fungal strain	1167:1198	The particular high antifungal performance of CuO/C nanocomposite was found against Aspergillus niger while compare to Aspergillus flavus fungal strain.					
31029334	8	21	theme	nanocomposite	1100:1112	arg1	performance					1079:1089	The particular high antifungal performance	1048:1089	The particular high antifungal performance of CuO/C nanocomposite	1048:1112	The particular high antifungal performance of CuO/C nanocomposite was found against Aspergillus niger while compare to Aspergillus flavus fungal strain.					
31029334	5	22	theme	Aspergillus	789:799	arg1	niger					801:805	Aspergillus niger	789:805	Aspergillus niger	789:805	The antifungal activities of CuO/C nanocomposite were tested against Aspergillus niger and Aspergillus flavus species.					
31029334	2	23	theme	green	301:305	arg1	environment					307:317	a green environment	299:317	a green environment which prevents aggregation of sucrose and promotes nanoparticles formation	299:392	The synthesis process was done in a green environment which prevents aggregation of sucrose and promotes nanoparticles formation.					
31029334	8	24	theme	CuO/C	1094:1098	arg1	nanocomposite					1100:1112	CuO/C nanocomposite	1094:1112	CuO/C nanocomposite	1094:1112	The particular high antifungal performance of CuO/C nanocomposite was found against Aspergillus niger while compare to Aspergillus flavus fungal strain.					
31029334	2	25	theme	nanoparticles	370:382	arg1	formation					384:392	nanoparticles formation	370:392	nanoparticles formation	370:392	The synthesis process was done in a green environment which prevents aggregation of sucrose and promotes nanoparticles formation.					
31029334	5	26	theme	antifungal	724:733	arg1	activities					735:744	The antifungal activities	720:744	The antifungal activities of CuO/C nanocomposite	720:767	The antifungal activities of CuO/C nanocomposite were tested against Aspergillus niger and Aspergillus flavus species.					
31029334	7	27	theme	bioactive	978:986	arg1	nanocomposite					994:1006	bioactive CuO/C nanocomposite	978:1006	bioactive CuO/C nanocomposite	978:1006	The fungal inhibition mechanism of bioactive CuO/C nanocomposite was discussed in this research article.					
31029334	3	28	theme	oxide	471:475	arg1	nanocomposite					499:511	CuO/C nanocomposite	493:511	CuO/C nanocomposite	493:511	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	3	28	theme	oxide	471:475	arg1	nanocomposites					477:490	copper oxide nanocomposites	464:490	copper oxide nanocomposites (CuO/C nanocomposite)	464:512	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	4	29	theme	CuO/C	574:578	arg1	nanocomposite					580:592	the produced CuO/C nanocomposite	561:592	the produced CuO/C nanocomposite	561:592	Additionally, the produced CuO/C nanocomposite were characterized using microscopic techniques like SEM, TEM and spectroscopic techniques like UV-vis and X-ray diffraction.					
31029334	5	30	dep	Aspergillus	811:821	arg1	flavus					823:828	flavus	823:828	flavus	823:828	The antifungal activities of CuO/C nanocomposite were tested against Aspergillus niger and Aspergillus flavus species.					
31029334	6	31	theme	A.	902:903	arg1	flavus					905:910	A. flavus	902:910	A. flavus	902:910	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	1	32	theme	CuO/C	135:139	arg1	composite					146:154	bioactive CuO/C nano composite	125:154	bioactive CuO/C nano composite using sucrose as a capping agent	125:187	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
31029334	4	33	theme	produced	565:572	arg1	nanocomposite					580:592	the produced CuO/C nanocomposite	561:592	the produced CuO/C nanocomposite	561:592	Additionally, the produced CuO/C nanocomposite were characterized using microscopic techniques like SEM, TEM and spectroscopic techniques like UV-vis and X-ray diffraction.					
31029334	8	34	theme	Aspergillus	1132:1142	arg1	niger					1144:1148	Aspergillus niger	1132:1148	Aspergillus niger	1132:1148	The particular high antifungal performance of CuO/C nanocomposite was found against Aspergillus niger while compare to Aspergillus flavus fungal strain.					
31029334	1	35	theme	capping	175:181	arg1	sucrose					162:168	sucrose	162:168	sucrose	162:168	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
31029334	1	35	theme	capping	175:181	arg1	agent					183:187	a capping agent	173:187	a capping agent	173:187	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
31029334	4	36	theme	spectroscopic	660:672	arg1	techniques					674:683	spectroscopic techniques	660:683	spectroscopic techniques like UV-vis and X-ray diffraction	660:717	Additionally, the produced CuO/C nanocomposite were characterized using microscopic techniques like SEM, TEM and spectroscopic techniques like UV-vis and X-ray diffraction.					
31029334	5	37	theme	CuO/C	749:753	arg1	nanocomposite					755:767	CuO/C nanocomposite	749:767	CuO/C nanocomposite	749:767	The antifungal activities of CuO/C nanocomposite were tested against Aspergillus niger and Aspergillus flavus species.					
31029334	8	38	dep	Aspergillus	1167:1177	arg1	flavus					1179:1184	flavus	1179:1184	flavus	1179:1184	The particular high antifungal performance of CuO/C nanocomposite was found against Aspergillus niger while compare to Aspergillus flavus fungal strain.					
31029334	3	39	theme	copper	464:469	arg1	nanocomposite					499:511	CuO/C nanocomposite	493:511	CuO/C nanocomposite	493:511	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	3	39	theme	copper	464:469	arg1	nanocomposites					477:490	copper oxide nanocomposites	464:490	copper oxide nanocomposites (CuO/C nanocomposite)	464:512	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	3	40	theme	innovative	399:408	arg1	approach					410:417	The innovative approach	395:417	The innovative approach	395:417	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	5	41	theme	nanocomposite	755:767	arg1	activities					735:744	The antifungal activities	720:744	The antifungal activities of CuO/C nanocomposite	720:767	The antifungal activities of CuO/C nanocomposite were tested against Aspergillus niger and Aspergillus flavus species.					
31029334	6	42	theme	%	887:887	arg1	restraint					889:897	70% restraint	885:897	70% restraint on A. flavus and 90% hindrance on A. niger	885:940	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	4	43	theme	microscopic	619:629	arg1	techniques					631:640	microscopic techniques	619:640	microscopic techniques like SEM, TEM and spectroscopic techniques like UV-vis and X-ray diffraction	619:717	Additionally, the produced CuO/C nanocomposite were characterized using microscopic techniques like SEM, TEM and spectroscopic techniques like UV-vis and X-ray diffraction.					
31029334	6	44	theme	70	885:886	arg1	%					887:887	%	887:887	%	887:887	At 1000 ppm of CuO/C nanocomposite, it showed 70% restraint on A. flavus and 90% hindrance on A. niger.					
31029334	8	45	theme	antifungal	1068:1077	arg1	performance					1079:1089	The particular high antifungal performance	1048:1089	The particular high antifungal performance of CuO/C nanocomposite	1048:1112	The particular high antifungal performance of CuO/C nanocomposite was found against Aspergillus niger while compare to Aspergillus flavus fungal strain.					
31029334	4	46	theme	X-ray	701:705	arg1	diffraction					707:717	X-ray diffraction	701:717	X-ray diffraction	701:717	Additionally, the produced CuO/C nanocomposite were characterized using microscopic techniques like SEM, TEM and spectroscopic techniques like UV-vis and X-ray diffraction.					
31029334	1	47	theme	simple	209:214	arg1	approach					222:229	simple green approach	209:229	simple green approach	209:229	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
31029334	7	48	theme	research	1030:1037	arg1	article					1039:1045	this research article	1025:1045	this research article	1025:1045	The fungal inhibition mechanism of bioactive CuO/C nanocomposite was discussed in this research article.					
31029334	1	49	theme	bioactive	125:133	arg1	composite					146:154	bioactive CuO/C nano composite	125:154	bioactive CuO/C nano composite using sucrose as a capping agent	125:187	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
31029334	1	50	theme	green	216:220	arg1	approach					222:229	simple green approach	209:229	simple green approach	209:229	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
31029334	0	51	theme	carbon	65:70	arg1	source					72:77	carbon source	65:77	carbon source	65:77	CuO/C nanocomposite: Synthesis and optimization using sucrose as carbon source and its antifungal activity.					
31029334	3	52	theme	particle	523:530	arg1	size					532:535	the particle size	519:535	the particle size of 50 nm	519:544	The innovative approach produces sucrose as a carbon source mediated copper oxide nanocomposites (CuO/C nanocomposite) with the particle size of 50 nm.					
31029334	8	53	theme	Aspergillus	1167:1177	arg1	strain					1193:1198	Aspergillus flavus fungal strain	1167:1198	Aspergillus flavus fungal strain	1167:1198	The particular high antifungal performance of CuO/C nanocomposite was found against Aspergillus niger while compare to Aspergillus flavus fungal strain.					
31029334	7	54	theme	nanocomposite	994:1006	arg1	mechanism					965:973	The fungal inhibition mechanism	943:973	The fungal inhibition mechanism of bioactive CuO/C nanocomposite	943:1006	The fungal inhibition mechanism of bioactive CuO/C nanocomposite was discussed in this research article.					
31029334	1	55	theme	nano	141:144	arg1	composite					146:154	bioactive CuO/C nano composite	125:154	bioactive CuO/C nano composite using sucrose as a capping agent	125:187	The synthesis of bioactive CuO/C nano composite using sucrose as a capping agent is achieved through simple green approach via Response Surface Methodology.					
30699337	4	0	theme	~7 ± 2 nm	654:662	arg1	size					664:667	~7 ± 2 nm size	654:667	~7 ± 2 nm size	654:667	TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7 ± 2 nm size.					
30699337	1	1	theme	significant	173:183	arg1	materials					199:207	significant antibacterial materials	173:207	significant antibacterial materials in the clinical field	173:229	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	1	1	theme	significant	173:183	arg1	nanomaterials					135:147	Chitosan (CH) capped inorganic nanomaterials	104:147	Chitosan (CH) capped inorganic nanomaterials	104:147	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	4	2	theme	TEM	544:546	arg1	analysis					548:555	TEM analysis	544:555	TEM analysis	544:555	TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7 ± 2 nm size.					
30699337	1	3	theme	antibacterial	185:197	arg1	materials					199:207	significant antibacterial materials	173:207	significant antibacterial materials in the clinical field	173:229	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	1	3	theme	antibacterial	185:197	arg1	nanomaterials					135:147	Chitosan (CH) capped inorganic nanomaterials	104:147	Chitosan (CH) capped inorganic nanomaterials	104:147	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	6	4	theme	CHCuO-CH	877:884	arg1	film					886:889	CHCuO-CH film	877:889	CHCuO-CH film	877:889	The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials.					
30699337	6	4	theme	CHCuO-CH	877:884	arg1	CHCuO					863:867	the CHCuO NPs and CHCuO-CH film	859:889	the CHCuO NPs and CHCuO-CH film	859:889	The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials.					
30699337	2	5	theme	oxide	366:370	arg1	incorporation					332:344	the incorporation	328:344	the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs)	328:416	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	3	6	theme	ascorbic	529:536	arg1	acid					538:541	ascorbic acid	529:541	ascorbic acid	529:541	Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid.					
30699337	3	7	theme	sodium	508:513	arg1	hydroxide					515:523	sodium hydroxide	508:523	sodium hydroxide	508:523	Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid.					
30699337	5	8	theme	developed	700:708	arg1	materials					710:718	the developed materials	696:718	the developed materials	696:718	Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria.					
30699337	2	9	theme	copper	359:364	arg1	CHCuO					373:377	CHCuO	373:377	CHCuO	373:377	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	2	9	theme	copper	359:364	arg1	oxide					366:370	CH capped copper oxide	349:370	CH capped copper oxide (CHCuO)	349:378	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	2	10	theme	films	307:311	arg1	synthesis					252:260	the synthesis	248:260	the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs)	248:416	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	4	11	theme	spherical	620:628	arg1	shape					630:634	a spherical shape	618:634	a spherical shape for CHCu NPs with ~7 ± 2 nm size	618:667	TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7 ± 2 nm size.					
30699337	5	12	theme	materials	710:718	arg1	activity					684:691	Antimicrobial activity	670:691	Antimicrobial activity of the developed materials	670:718	Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria.					
30699337	4	13	with	NPs	645:647	arg1	size					664:667	~7 ± 2 nm size	654:667	~7 ± 2 nm size	654:667	TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7 ± 2 nm size.					
30699337	5	14	theme	inhibition	739:748	arg1	method					755:760	the inhibition zone method	735:760	the inhibition zone method	735:760	Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria.					
30699337	2	15	theme	composite	297:305	arg1	films					307:311	two new different antibacterial composite films	265:311	two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs)	265:416	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	0	16	theme	capped	9:14	arg1	nanoparticles					36:48	Chitosan capped copper oxide/copper nanoparticles	0:48	Chitosan capped copper oxide/copper nanoparticles	0:48	Chitosan capped copper oxide/copper nanoparticles encapsulated microbial resistant nanocomposite films.					
30699337	2	17	theme	incorporation	332:344	arg1	result					318:323	a result	316:323	a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs)	316:416	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	2	18	theme	copper	384:389	arg1	nanoparticles					398:410	copper (CHCu) nanoparticles	384:410	copper (CHCu) nanoparticles (NPs)	384:416	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	2	18	theme	copper	384:389	arg1	NPs					413:415	NPs	413:415	NPs	413:415	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	0	19	theme	Chitosan	0:7	arg1	nanoparticles					36:48	Chitosan capped copper oxide/copper nanoparticles	0:48	Chitosan capped copper oxide/copper nanoparticles	0:48	Chitosan capped copper oxide/copper nanoparticles encapsulated microbial resistant nanocomposite films.					
30699337	1	20	theme	clinical	216:223	arg1	field					225:229	the clinical field	212:229	the clinical field	212:229	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	0	21	theme	oxide/copper	23:34	arg1	nanoparticles					36:48	Chitosan capped copper oxide/copper nanoparticles	0:48	Chitosan capped copper oxide/copper nanoparticles	0:48	Chitosan capped copper oxide/copper nanoparticles encapsulated microbial resistant nanocomposite films.					
30699337	5	22	theme	gram-positive	794:806	arg1	bacteria					808:815	both gram-negative and gram-positive bacteria	771:815	both gram-negative and gram-positive bacteria	771:815	Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria.					
30699337	0	23	theme	copper	16:21	arg1	nanoparticles					36:48	Chitosan capped copper oxide/copper nanoparticles	0:48	Chitosan capped copper oxide/copper nanoparticles	0:48	Chitosan capped copper oxide/copper nanoparticles encapsulated microbial resistant nanocomposite films.					
30699337	6	24	theme	other	932:936	arg1	nanomaterials					938:950	the other nanomaterials	928:950	the other nanomaterials	928:950	The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials.					
30699337	3	25	theme	Cu2+	492:495	arg1	ions					497:500	Cu2+ ions	492:500	Cu2+ ions using sodium hydroxide and ascorbic acid	492:541	Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid.					
30699337	1	26	theme	capped	118:123	arg1	materials					199:207	significant antibacterial materials	173:207	significant antibacterial materials in the clinical field	173:229	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	1	26	theme	capped	118:123	arg1	nanomaterials					135:147	Chitosan (CH) capped inorganic nanomaterials	104:147	Chitosan (CH) capped inorganic nanomaterials	104:147	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	3	27	theme	facile	463:468	arg1	reduction					479:487	a facile chemical reduction	461:487	a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid	461:541	Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid.					
30699337	5	28	theme	Antimicrobial	670:682	arg1	activity					684:691	Antimicrobial activity	670:691	Antimicrobial activity of the developed materials	670:718	Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria.					
30699337	2	29	theme	nanoparticles	398:410	arg1	incorporation					332:344	the incorporation	328:344	the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs)	328:416	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	5	30	theme	zone	750:753	arg1	method					755:760	the inhibition zone method	735:760	the inhibition zone method	735:760	Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria.					
30699337	5	31	theme	gram-negative	776:788	arg1	bacteria					808:815	both gram-negative and gram-positive bacteria	771:815	both gram-negative and gram-positive bacteria	771:815	Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria.					
30699337	0	32	theme	resistant	73:81	arg1	films					97:101	microbial resistant nanocomposite films	63:101	microbial resistant nanocomposite films	63:101	Chitosan capped copper oxide/copper nanoparticles encapsulated microbial resistant nanocomposite films.					
30699337	6	33	theme	antimicrobial	822:834	arg1	activity					836:843	The antimicrobial activity	818:843	The antimicrobial activity	818:843	The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials.					
30699337	1	34	theme	inorganic	125:133	arg1	materials					199:207	significant antibacterial materials	173:207	significant antibacterial materials in the clinical field	173:229	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	1	34	theme	inorganic	125:133	arg1	nanomaterials					135:147	Chitosan (CH) capped inorganic nanomaterials	104:147	Chitosan (CH) capped inorganic nanomaterials	104:147	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	0	35	theme	microbial	63:71	arg1	films					97:101	microbial resistant nanocomposite films	63:101	microbial resistant nanocomposite films	63:101	Chitosan capped copper oxide/copper nanoparticles encapsulated microbial resistant nanocomposite films.					
30699337	6	36	theme	inhibition	907:916	arg1	zone					918:921	a higher inhibition zone	898:921	a higher inhibition zone than the other nanomaterials	898:950	The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials.					
30699337	7	37	theme	wound	1021:1025	arg1	applications					1036:1047	wound dressing applications	1021:1047	wound dressing applications	1021:1047	The results suggested that the synthesized materials can be used in wound dressing applications.					
30699337	3	38	theme	chemical	470:477	arg1	reduction					479:487	a facile chemical reduction	461:487	a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid	461:541	Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid.					
30699337	2	39	theme	capped	352:357	arg1	CHCuO					373:377	CHCuO	373:377	CHCuO	373:377	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	2	39	theme	capped	352:357	arg1	oxide					366:370	CH capped copper oxide	349:370	CH capped copper oxide (CHCuO)	349:378	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	6	40	theme	higher	900:905	arg1	zone					918:921	a higher inhibition zone	898:921	a higher inhibition zone than the other nanomaterials	898:950	The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials.					
30699337	3	41	dep	CHCuO	425:429	arg1	NPs					440:442	NPs	440:442	NPs	440:442	Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid.					
30699337	2	42	theme	antibacterial	283:295	arg1	films					307:311	two new different antibacterial composite films	265:311	two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs)	265:416	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	7	43	theme	synthesized	984:994	arg1	materials					996:1004	the synthesized materials	980:1004	the synthesized materials	980:1004	The results suggested that the synthesized materials can be used in wound dressing applications.					
30699337	2	44	theme	CH	349:350	arg1	CHCuO					373:377	CHCuO	373:377	CHCuO	373:377	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	2	44	theme	CH	349:350	arg1	oxide					366:370	CH capped copper oxide	349:370	CH capped copper oxide (CHCuO)	349:378	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	6	45	dep	CHCuO	863:867	arg1	NPs					869:871	NPs	869:871	NPs	869:871	The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials.					
30699337	6	45	dep	CHCuO	863:867	arg1	film					886:889	CHCuO-CH film	877:889	CHCuO-CH film	877:889	The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials.					
30699337	6	45	dep	CHCuO	863:867	arg1	CHCuO					863:867	the CHCuO NPs and CHCuO-CH film	859:889	the CHCuO NPs and CHCuO-CH film	859:889	The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials.					
30699337	3	46	theme	ions	497:500	arg1	reduction					479:487	a facile chemical reduction	461:487	a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid	461:541	Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu2+ ions using sodium hydroxide and ascorbic acid.					
30699337	2	47	theme	different	273:281	arg1	films					307:311	two new different antibacterial composite films	265:311	two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs)	265:416	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	2	48	theme	new	269:271	arg1	films					307:311	two new different antibacterial composite films	265:311	two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs)	265:416	This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs).					
30699337	7	49	used	used	1013:1016	arg2	materials					996:1004	the synthesized materials	980:1004	the synthesized materials	980:1004	The results suggested that the synthesized materials can be used in wound dressing applications.					
30699337	4	50	theme	rod-type	584:591	arg1	nanoflakes					593:602	rod-type nanoflakes	584:602	rod-type nanoflakes for CHCuO	584:612	TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7 ± 2 nm size.					
30699337	0	51	theme	nanocomposite	83:95	arg1	films					97:101	microbial resistant nanocomposite films	63:101	microbial resistant nanocomposite films	63:101	Chitosan capped copper oxide/copper nanoparticles encapsulated microbial resistant nanocomposite films.					
30699337	7	52	theme	dressing	1027:1034	arg1	applications					1036:1047	wound dressing applications	1021:1047	wound dressing applications	1021:1047	The results suggested that the synthesized materials can be used in wound dressing applications.					
30699337	1	53	from	materials	199:207	arg1	field					225:229	the clinical field	212:229	the clinical field	212:229	Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field.					
30699337	4	54	theme	CHCu	640:643	arg1	NPs					645:647	CHCu NPs	640:647	CHCu NPs with ~7 ± 2 nm size	640:667	TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7 ± 2 nm size.					
31350933	0	0	theme	glycine-rich	90:101	arg1	CpGRP1					111:116	the glycine-rich protein CpGRP1	86:116	the glycine-rich protein CpGRP1	86:116	Craterostigma plantagineum cell wall composition is remodelled during desiccation and the glycine-rich protein CpGRP1 interacts with pectins through clustered arginines.					
31350933	9	1	theme	phosphatidic	1418:1429	arg1	acid					1431:1434	phosphatidic acid	1418:1434	phosphatidic acid (PA)	1418:1439	CpGRP1 can also bind phosphatidic acid (PA) and cardiolipin.					
31350933	9	1	theme	phosphatidic	1418:1429	arg1	PA					1437:1438	PA	1437:1438	PA	1437:1438	CpGRP1 can also bind phosphatidic acid (PA) and cardiolipin.					
31350933	10	2	theme	higher	1569:1574	arg1	affinity					1576:1583	a higher affinity	1567:1583	a higher affinity to pectin than its binding partner CpWAK1	1567:1625	The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1.					
31350933	10	3	theme	CpGRP1	1473:1478	arg1	binding					1462:1468	The binding	1458:1468	The binding of CpGRP1 to pectin	1458:1488	The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1.					
31350933	10	3	theme	CpGRP1	1473:1478	arg1	dependent					1504:1512	dependent	1504:1512	dependent	1504:1512	The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1.					
31350933	8	4	theme	de-methylesterified	1270:1288	arg1	pectin					1290:1295	de-methylesterified pectin	1270:1295	de-methylesterified pectin	1270:1295	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	8	5	theme	residues	1257:1264	arg1	cluster					1237:1243	a cluster	1235:1243	a cluster of arginine residues	1235:1264	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	4	6	theme	rehydrated	548:557	arg1	sections					564:571	hydrated, desiccated and rehydrated leaf sections	523:571	hydrated, desiccated and rehydrated leaf sections	523:571	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy.					
31350933	5	7	theme	different	645:653	arg1	fractions					665:673	different cell wall fractions	645:673	different cell wall fractions	645:673	Pectin composition in different cell wall fractions was analysed with monoclonal antibodies against homogalacturonan, rhamnogalacturonan I, rhamnogalacturonan II and hemicellulose epitopes.					
31350933	10	8	theme	methylesterification	1528:1547	arg1	status					1549:1554	the pectin methylesterification status	1517:1554	the pectin methylesterification status	1517:1554	The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1.					
31350933	5	9	theme	cell	655:658	arg1	fractions					665:673	different cell wall fractions	645:673	different cell wall fractions	645:673	Pectin composition in different cell wall fractions was analysed with monoclonal antibodies against homogalacturonan, rhamnogalacturonan I, rhamnogalacturonan II and hemicellulose epitopes.					
31350933	0	10	theme	protein	103:109	arg1	CpGRP1					111:116	the glycine-rich protein CpGRP1	86:116	the glycine-rich protein CpGRP1	86:116	Craterostigma plantagineum cell wall composition is remodelled during desiccation and the glycine-rich protein CpGRP1 interacts with pectins through clustered arginines.					
31350933	11	11	theme	folding	1800:1806	arg1	activation					1747:1756	the activation	1743:1756	the activation of dehydration-related responses and leaf folding	1743:1806	It is hypothesised that changes in pectin composition are sensed by the CpGRP1-CpWAK1 complex therefore leading to the activation of dehydration-related responses and leaf folding.					
31350933	8	12	theme	cell	1164:1167	arg1	adaptations					1169:1179	cell adaptations	1164:1179	cell adaptations to water deficit	1164:1196	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	5	13	theme	wall	660:663	arg1	fractions					665:673	different cell wall fractions	645:673	different cell wall fractions	645:673	Pectin composition in different cell wall fractions was analysed with monoclonal antibodies against homogalacturonan, rhamnogalacturonan I, rhamnogalacturonan II and hemicellulose epitopes.					
31350933	11	14	from	changes	1652:1658	arg1	composition					1670:1680	pectin composition	1663:1680	pectin composition	1663:1680	It is hypothesised that changes in pectin composition are sensed by the CpGRP1-CpWAK1 complex therefore leading to the activation of dehydration-related responses and leaf folding.					
31350933	3	15	theme	wall	384:387	arg1	composition					396:406	cell wall pectin composition	379:406	cell wall pectin composition	379:406	To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed.					
31350933	4	16	theme	microstructural	496:510	arg1	changes					512:518	Cellular microstructural changes	487:518	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections	487:571	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy.					
31350933	8	17	theme	water	1184:1188	arg1	deficit					1190:1196	water deficit	1184:1196	water deficit	1184:1196	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	7	18	theme	rhamnogalacturonan	1083:1100	arg1	detection					1048:1056	the detection	1044:1056	the detection of rhamnogalacturonan I, rhamnogalacturonan II and hemicelluloses	1044:1122	Homogalacturonan was less methylesterified upon desiccation and changes were also demonstrated in the detection of rhamnogalacturonan I, rhamnogalacturonan II and hemicelluloses.					
31350933	12	19	theme	CpGRP1	1851:1856	arg1	activity					1858:1865	CpGRP1 activity	1851:1865	CpGRP1 activity	1851:1865	PA might participate in the modulation of CpGRP1 activity.					
31350933	10	20	contain	has	1563:1565	arg2	affinity					1576:1583	a higher affinity	1567:1583	a higher affinity to pectin than its binding partner CpWAK1	1567:1625	The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1.					
31350933	10	20	contain	has	1563:1565	arg1	it					1560:1561	it	1560:1561	it	1560:1561	The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1.					
31350933	11	21	theme	dehydration-related	1761:1779	arg1	responses					1781:1789	dehydration-related responses	1761:1789	dehydration-related responses	1761:1789	It is hypothesised that changes in pectin composition are sensed by the CpGRP1-CpWAK1 complex therefore leading to the activation of dehydration-related responses and leaf folding.					
31350933	3	22	theme	pectin	389:394	arg1	composition					396:406	cell wall pectin composition	379:406	cell wall pectin composition	379:406	To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed.					
31350933	4	23	theme	hydrated	523:530	arg1	sections					564:571	hydrated, desiccated and rehydrated leaf sections	523:571	hydrated, desiccated and rehydrated leaf sections	523:571	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy.					
31350933	7	24	theme	rhamnogalacturonan	1061:1078	arg1	detection					1048:1056	the detection	1044:1056	the detection of rhamnogalacturonan I, rhamnogalacturonan II and hemicelluloses	1044:1122	Homogalacturonan was less methylesterified upon desiccation and changes were also demonstrated in the detection of rhamnogalacturonan I, rhamnogalacturonan II and hemicelluloses.					
31350933	8	25	theme	binding	1311:1317	arg1	sites					1319:1323	more binding sites	1306:1323	more binding sites	1306:1323	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	8	26	theme	more	1306:1309	arg1	sites					1319:1323	more binding sites	1306:1323	more binding sites	1306:1323	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	0	27	theme	plantagineum	14:25	arg1	composition					37:47	Craterostigma plantagineum cell wall composition	0:47	Craterostigma plantagineum cell wall composition	0:47	Craterostigma plantagineum cell wall composition is remodelled during desiccation and the glycine-rich protein CpGRP1 interacts with pectins through clustered arginines.					
31350933	11	28	theme	leaf	1795:1798	arg1	folding					1800:1806	leaf folding	1795:1806	leaf folding	1795:1806	It is hypothesised that changes in pectin composition are sensed by the CpGRP1-CpWAK1 complex therefore leading to the activation of dehydration-related responses and leaf folding.					
31350933	4	29	from	changes	512:518	arg1	sections					564:571	hydrated, desiccated and rehydrated leaf sections	523:571	hydrated, desiccated and rehydrated leaf sections	523:571	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy.					
31350933	5	30	theme	Pectin	623:628	arg1	composition					630:640	Pectin composition	623:640	Pectin composition in different cell wall fractions	623:673	Pectin composition in different cell wall fractions was analysed with monoclonal antibodies against homogalacturonan, rhamnogalacturonan I, rhamnogalacturonan II and hemicellulose epitopes.					
31350933	1	31	theme	desiccation-tolerant	212:231	arg1	plants					244:249	the desiccation-tolerant angiosperm plants	208:249	the desiccation-tolerant angiosperm plants	208:249	Craterostigma plantagineum belongs to the desiccation-tolerant angiosperm plants.					
31350933	0	32	theme	Craterostigma	0:12	arg1	composition					37:47	Craterostigma plantagineum cell wall composition	0:47	Craterostigma plantagineum cell wall composition	0:47	Craterostigma plantagineum cell wall composition is remodelled during desiccation and the glycine-rich protein CpGRP1 interacts with pectins through clustered arginines.					
31350933	4	33	theme	scanning	593:600	arg1	microscopy					611:620	scanning electron microscopy	593:620	scanning electron microscopy	593:620	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy.					
31350933	5	34	theme	monoclonal	693:702	arg1	antibodies					704:713	monoclonal antibodies	693:713	monoclonal antibodies against homogalacturonan, rhamnogalacturonan I, rhamnogalacturonan II and hemicellulose epitopes	693:810	Pectin composition in different cell wall fractions was analysed with monoclonal antibodies against homogalacturonan, rhamnogalacturonan I, rhamnogalacturonan II and hemicellulose epitopes.					
31350933	8	35	theme	central	1148:1154	arg1	role					1156:1159	a central role	1146:1159	a central role	1146:1159	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	8	36	dep	hydrated	1380:1387	arg1	leaves					1389:1394	leaves	1389:1394	leaves	1389:1394	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	1	37	theme	angiosperm	233:242	arg1	plants					244:249	the desiccation-tolerant angiosperm plants	208:249	the desiccation-tolerant angiosperm plants	208:249	Craterostigma plantagineum belongs to the desiccation-tolerant angiosperm plants.					
31350933	0	38	theme	wall	32:35	arg1	composition					37:47	Craterostigma plantagineum cell wall composition	0:47	Craterostigma plantagineum cell wall composition	0:47	Craterostigma plantagineum cell wall composition is remodelled during desiccation and the glycine-rich protein CpGRP1 interacts with pectins through clustered arginines.					
31350933	10	39	theme	pectin	1521:1526	arg1	status					1549:1554	the pectin methylesterification status	1517:1554	the pectin methylesterification status	1517:1554	The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1.					
31350933	3	40	dep	process	360:366	arg1	changes					368:374	changes	368:374	changes in cell wall pectin composition	368:406	To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed.					
31350933	6	41	theme	cell	924:927	arg1	properties					934:943	cell wall properties	924:943	cell wall properties	924:943	Our data demonstrate changes in pectin composition during dehydration/rehydration which is suggested to affect cell wall properties.					
31350933	0	42	theme	cell	27:30	arg1	composition					37:47	Craterostigma plantagineum cell wall composition	0:47	Craterostigma plantagineum cell wall composition	0:47	Craterostigma plantagineum cell wall composition is remodelled during desiccation and the glycine-rich protein CpGRP1 interacts with pectins through clustered arginines.					
31350933	10	43	theme	binding	1604:1610	arg1	CpWAK1					1620:1625	its binding partner CpWAK1	1600:1625	its binding partner CpWAK1	1600:1625	The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1.					
31350933	3	44	theme	glycine-rich	440:451	arg1	CpGRP1					464:469	CpGRP1	464:469	CpGRP1	464:469	To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed.					
31350933	3	44	theme	glycine-rich	440:451	arg1	protein					453:459	apoplastic glycine-rich protein 1	429:461	the apoplastic glycine-rich protein 1 (CpGRP1)	425:470	To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed.					
31350933	4	45	theme	electron	602:609	arg1	microscopy					611:620	scanning electron microscopy	593:620	scanning electron microscopy	593:620	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy.					
31350933	5	46	theme	hemicellulose	789:801	arg1	epitopes					803:810	hemicellulose epitopes	789:810	hemicellulose epitopes	789:810	Pectin composition in different cell wall fractions was analysed with monoclonal antibodies against homogalacturonan, rhamnogalacturonan I, rhamnogalacturonan II and hemicellulose epitopes.					
31350933	3	47	theme	protein	453:459	arg1	role					417:420	the role	413:420	the role of the apoplastic glycine-rich protein 1 (CpGRP1)	413:470	To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed.					
31350933	8	48	from	hydrated	1380:1387	arg1	pectin					1368:1373	pectin	1368:1373	pectin from hydrated leaves	1368:1394	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	0	49	theme	clustered	149:157	arg1	arginines					159:167	clustered arginines	149:167	clustered arginines	149:167	Craterostigma plantagineum cell wall composition is remodelled during desiccation and the glycine-rich protein CpGRP1 interacts with pectins through clustered arginines.					
31350933	4	50	theme	Cellular	487:494	arg1	changes					512:518	Cellular microstructural changes	487:518	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections	487:571	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy.					
31350933	11	51	theme	CpGRP1-CpWAK1	1700:1712	arg1	complex					1714:1720	the CpGRP1-CpWAK1 complex	1696:1720	the CpGRP1-CpWAK1 complex therefore leading to the activation of dehydration-related responses and leaf folding	1696:1806	It is hypothesised that changes in pectin composition are sensed by the CpGRP1-CpWAK1 complex therefore leading to the activation of dehydration-related responses and leaf folding.					
31350933	4	52	theme	leaf	559:562	arg1	sections					564:571	hydrated, desiccated and rehydrated leaf sections	523:571	hydrated, desiccated and rehydrated leaf sections	523:571	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy.					
31350933	8	53	theme	protein-pectin	1333:1346	arg1	interaction					1348:1358	the protein-pectin interaction	1329:1358	the protein-pectin interaction than to pectin from hydrated leaves	1329:1394	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	6	54	theme	pectin	845:850	arg1	composition					852:862	pectin composition	845:862	pectin composition	845:862	Our data demonstrate changes in pectin composition during dehydration/rehydration which is suggested to affect cell wall properties.					
31350933	5	55	from	composition	630:640	arg1	fractions					665:673	different cell wall fractions	645:673	different cell wall fractions	645:673	Pectin composition in different cell wall fractions was analysed with monoclonal antibodies against homogalacturonan, rhamnogalacturonan I, rhamnogalacturonan II and hemicellulose epitopes.					
31350933	3	56	theme	apoplastic	429:438	arg1	CpGRP1					464:469	CpGRP1	464:469	CpGRP1	464:469	To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed.					
31350933	3	56	theme	apoplastic	429:438	arg1	protein					453:459	apoplastic glycine-rich protein 1	429:461	the apoplastic glycine-rich protein 1 (CpGRP1)	425:470	To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed.					
31350933	10	57	theme	partner	1612:1618	arg1	CpWAK1					1620:1625	its binding partner CpWAK1	1600:1625	its binding partner CpWAK1	1600:1625	The binding of CpGRP1 to pectin appears to be dependent on the pectin methylesterification status and it has a higher affinity to pectin than its binding partner CpWAK1.					
31350933	6	58	from	changes	834:840	arg1	composition					852:862	pectin composition	845:862	pectin composition	845:862	Our data demonstrate changes in pectin composition during dehydration/rehydration which is suggested to affect cell wall properties.					
31350933	11	59	theme	pectin	1663:1668	arg1	composition					1670:1680	pectin composition	1663:1680	pectin composition	1663:1680	It is hypothesised that changes in pectin composition are sensed by the CpGRP1-CpWAK1 complex therefore leading to the activation of dehydration-related responses and leaf folding.					
31350933	11	60	theme	responses	1781:1789	arg1	activation					1747:1756	the activation	1743:1756	the activation of dehydration-related responses and leaf folding	1743:1806	It is hypothesised that changes in pectin composition are sensed by the CpGRP1-CpWAK1 complex therefore leading to the activation of dehydration-related responses and leaf folding.					
31350933	8	61	theme	arginine	1248:1255	arg1	residues					1257:1264	arginine residues	1248:1264	arginine residues	1248:1264	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	6	62	theme	wall	929:932	arg1	properties					934:943	cell wall properties	924:943	cell wall properties	924:943	Our data demonstrate changes in pectin composition during dehydration/rehydration which is suggested to affect cell wall properties.					
31350933	3	63	theme	cell	379:382	arg1	composition					396:406	cell wall pectin composition	379:406	cell wall pectin composition	379:406	To understand this process changes in cell wall pectin composition, and the role of the apoplastic glycine-rich protein 1 (CpGRP1) were analysed.					
31350933	4	64	theme	desiccated	533:542	arg1	sections					564:571	hydrated, desiccated and rehydrated leaf sections	523:571	hydrated, desiccated and rehydrated leaf sections	523:571	Cellular microstructural changes in hydrated, desiccated and rehydrated leaf sections were analysed using scanning electron microscopy.					
31350933	7	65	theme	hemicelluloses	1109:1122	arg1	detection					1048:1056	the detection	1044:1056	the detection of rhamnogalacturonan I, rhamnogalacturonan II and hemicelluloses	1044:1122	Homogalacturonan was less methylesterified upon desiccation and changes were also demonstrated in the detection of rhamnogalacturonan I, rhamnogalacturonan II and hemicelluloses.					
31350933	1	66	theme	Craterostigma	170:182	arg1	plantagineum					184:195	Craterostigma plantagineum	170:195	Craterostigma plantagineum	170:195	Craterostigma plantagineum belongs to the desiccation-tolerant angiosperm plants.					
31350933	12	67	theme	activity	1858:1865	arg1	modulation					1837:1846	the modulation	1833:1846	the modulation of CpGRP1 activity	1833:1865	PA might participate in the modulation of CpGRP1 activity.					
31350933	8	68	contain	have	1141:1144	arg1	CpGRP1					1125:1130	CpGRP1	1125:1130	CpGRP1	1125:1130	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
31350933	8	68	contain	have	1141:1144	arg2	role					1156:1159	a central role	1146:1159	a central role	1146:1159	CpGRP1 seems to have a central role in cell adaptations to water deficit, as it interacts with pectin through a cluster of arginine residues and de-methylesterified pectin presents more binding sites for the protein-pectin interaction than to pectin from hydrated leaves.					
30707707	0	0	theme	stalk-specific	86:99	arg1	peptidoglycan					101:113	stalk-specific peptidoglycan	86:113	stalk-specific peptidoglycan	86:113	A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.					
30707707	6	1	dep	divisome	1150:1157	arg1	the					1146:1148	the	1146:1148	the	1146:1148	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	4	2	theme	specific	779:786	arg1	properties					799:808	specific mechanical properties	779:808	specific mechanical properties	779:808	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	4	3	theme	cell	582:585	arg1	body					587:590	cell body	582:590	cell body	582:590	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	2	4	theme	surface	385:391	arg1	attachment					393:402	surface attachment	385:402	surface attachment	385:402	Caulobacter crescentus, for instance, exhibits a stalk-like extension that carries an adhesive holdfast mediating surface attachment.					
30707707	6	5	theme	specific	1123:1130	arg1	components					1132:1141	specific components	1123:1141	specific components of the divisome and elongasome	1123:1172	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	6	6	theme	morphological	1279:1291	arg1	traits					1293:1298	new morphological traits	1275:1298	new morphological traits	1275:1298	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	0	7	theme	peptidoglycan	101:113	arg1	formation					73:81	the formation	69:81	the formation of stalk-specific peptidoglycan in Caulobacter crescentus	69:139	A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.					
30707707	5	8	theme	cell	940:943	arg1	machinery					963:971	the cell wall biosynthetic machinery	936:971	the cell wall biosynthetic machinery	936:971	To identify factors that participate in stalk formation, we systematically inactivated and localized predicted components of the cell wall biosynthetic machinery of C. crescentus.					
30707707	1	9	theme	distinctive	217:227	arg1	morphologies					229:240	their distinctive morphologies	211:240	their distinctive morphologies	211:240	Many bacteria have complex cell shapes, but the mechanisms producing their distinctive morphologies are still poorly understood.					
30707707	4	10	theme	distinct	746:753	arg1	domain					767:772	a distinct subcellular domain	744:772	a distinct subcellular domain with specific mechanical properties	744:808	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	5	11	theme	machinery	963:971	arg1	components					922:931	predicted components	912:931	predicted components of the cell wall biosynthetic machinery of C. crescentus	912:988	To identify factors that participate in stalk formation, we systematically inactivated and localized predicted components of the cell wall biosynthetic machinery of C. crescentus.					
30707707	0	12	theme	Caulobacter	118:128	arg1	crescentus					130:139	Caulobacter crescentus	118:139	Caulobacter crescentus	118:139	A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.					
30707707	4	13	with	domain	767:772	arg1	properties					799:808	specific mechanical properties	779:808	specific mechanical properties	779:808	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	5	14	theme	predicted	912:920	arg1	components					922:931	predicted components	912:931	predicted components of the cell wall biosynthetic machinery of C. crescentus	912:988	To identify factors that participate in stalk formation, we systematically inactivated and localized predicted components of the cell wall biosynthetic machinery of C. crescentus.					
30707707	6	15	theme	machinery	1222:1230	arg1	repurposing					1195:1205	the repurposing	1191:1205	the repurposing of preexisting machinery	1191:1230	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	6	16	theme	peptidoglycan	1039:1051	arg1	biosynthesis					1017:1028	the biosynthesis	1013:1028	the biosynthesis of stalk peptidoglycan	1013:1051	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	0	17	theme	specialized	2:12	arg1	complex					52:58	A specialized MreB-dependent cell wall biosynthetic complex	0:58	A specialized MreB-dependent cell wall biosynthetic complex	0:58	A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.					
30707707	6	18	theme	dedicated	1064:1072	arg1	complex					1101:1107	a dedicated peptidoglycan biosynthetic complex	1062:1107	a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits	1062:1298	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	6	19	theme	preexisting	1210:1220	arg1	machinery					1222:1230	preexisting machinery	1210:1230	preexisting machinery	1210:1230	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	3	20	theme	old	474:476	arg1	pole					483:486	the old cell pole	470:486	the old cell pole	470:486	This structure forms through zonal peptidoglycan biosynthesis at the old cell pole and elongates extensively under phosphate-limiting conditions.					
30707707	3	21	theme	zonal	434:438	arg1	biosynthesis					454:465	zonal peptidoglycan biosynthesis	434:465	zonal peptidoglycan biosynthesis	434:465	This structure forms through zonal peptidoglycan biosynthesis at the old cell pole and elongates extensively under phosphate-limiting conditions.					
30707707	6	22	theme	stalk	1033:1037	arg1	peptidoglycan					1039:1051	stalk peptidoglycan	1033:1051	stalk peptidoglycan	1033:1051	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	3	23	theme	cell	478:481	arg1	pole					483:486	the old cell pole	470:486	the old cell pole	470:486	This structure forms through zonal peptidoglycan biosynthesis at the old cell pole and elongates extensively under phosphate-limiting conditions.					
30707707	0	24	theme	cell	29:32	arg1	wall					34:37	MreB-dependent cell wall	14:37	A specialized MreB-dependent cell wall biosynthetic complex	0:58	A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.					
30707707	6	25	theme	biosynthetic	1088:1099	arg1	complex					1101:1107	a dedicated peptidoglycan biosynthetic complex	1062:1107	a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits	1062:1298	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	4	26	theme	mechanical	788:797	arg1	properties					799:808	specific mechanical properties	779:808	specific mechanical properties	779:808	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	0	27	theme	MreB-dependent	14:27	arg1	wall					34:37	MreB-dependent cell wall	14:37	A specialized MreB-dependent cell wall biosynthetic complex	0:58	A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.					
30707707	6	28	theme	peptidoglycan	1074:1086	arg1	complex					1101:1107	a dedicated peptidoglycan biosynthetic complex	1062:1107	a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits	1062:1298	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	2	29	theme	stalk-like	320:329	arg1	extension					331:339	a stalk-like extension	318:339	a stalk-like extension that carries an adhesive holdfast mediating surface attachment	318:402	Caulobacter crescentus, for instance, exhibits a stalk-like extension that carries an adhesive holdfast mediating surface attachment.					
30707707	3	30	theme	phosphate-limiting	520:537	arg1	conditions					539:548	phosphate-limiting conditions	520:548	phosphate-limiting conditions	520:548	This structure forms through zonal peptidoglycan biosynthesis at the old cell pole and elongates extensively under phosphate-limiting conditions.					
30707707	0	31	theme	biosynthetic	39:50	arg1	complex					52:58	A specialized MreB-dependent cell wall biosynthetic complex	0:58	A specialized MreB-dependent cell wall biosynthetic complex	0:58	A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.					
30707707	4	32	theme	significant	631:641	arg1	differences					643:653	significant differences	631:653	significant differences in the nature and proportion of peptide crosslinks	631:704	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	4	33	dep	nature	662:667	arg1	the					658:660	the	658:660	the	658:660	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	5	34	theme	crescentus	979:988	arg1	components					922:931	predicted components	912:931	predicted components of the cell wall biosynthetic machinery of C. crescentus	912:988	To identify factors that participate in stalk formation, we systematically inactivated and localized predicted components of the cell wall biosynthetic machinery of C. crescentus.					
30707707	5	35	theme	wall	945:948	arg1	machinery					963:971	the cell wall biosynthetic machinery	936:971	the cell wall biosynthetic machinery	936:971	To identify factors that participate in stalk formation, we systematically inactivated and localized predicted components of the cell wall biosynthetic machinery of C. crescentus.					
30707707	0	36	theme	wall	34:37	arg1	complex					52:58	A specialized MreB-dependent cell wall biosynthetic complex	0:58	A specialized MreB-dependent cell wall biosynthetic complex	0:58	A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.					
30707707	0	37	from	formation	73:81	arg1	crescentus					130:139	Caulobacter crescentus	118:139	Caulobacter crescentus	118:139	A specialized MreB-dependent cell wall biosynthetic complex mediates the formation of stalk-specific peptidoglycan in Caulobacter crescentus.					
30707707	4	38	theme	stalk	596:600	arg1	peptidoglycan					602:614	stalk peptidoglycan	596:614	stalk peptidoglycan	596:614	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	3	39	theme	peptidoglycan	440:452	arg1	biosynthesis					454:465	zonal peptidoglycan biosynthesis	434:465	zonal peptidoglycan biosynthesis	434:465	This structure forms through zonal peptidoglycan biosynthesis at the old cell pole and elongates extensively under phosphate-limiting conditions.					
30707707	4	40	theme	body	587:590	arg1	composition					567:577	the composition	563:577	the composition of cell body and stalk peptidoglycan	563:614	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	6	41	theme	elongasome	1163:1172	arg1	components					1132:1141	specific components	1123:1141	specific components of the divisome and elongasome	1123:1172	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	6	42	theme	straightforward	1243:1257	arg1	means					1259:1263	a straightforward means	1241:1263	a straightforward means to evolve new morphological traits	1241:1298	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	6	43	theme	new	1275:1277	arg1	traits					1293:1298	new morphological traits	1275:1298	new morphological traits	1275:1298	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	4	44	theme	peptidoglycan	602:614	arg1	composition					567:577	the composition	563:577	the composition of cell body and stalk peptidoglycan	563:614	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	4	45	from	differences	643:653	arg1	nature					662:667	nature	662:667	nature	662:667	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	4	45	from	differences	643:653	arg1	proportion					673:682	proportion	673:682	proportion	673:682	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	1	46	theme	Many	142:145	arg1	bacteria					147:154	Many bacteria	142:154	Many bacteria	142:154	Many bacteria have complex cell shapes, but the mechanisms producing their distinctive morphologies are still poorly understood.					
30707707	2	47	contain	carries	346:352	arg1	extension					331:339	a stalk-like extension	318:339	a stalk-like extension that carries an adhesive holdfast mediating surface attachment	318:402	Caulobacter crescentus, for instance, exhibits a stalk-like extension that carries an adhesive holdfast mediating surface attachment.					
30707707	2	47	contain	carries	346:352	arg2	holdfast					366:373	an adhesive holdfast	354:373	an adhesive holdfast mediating surface attachment	354:402	Caulobacter crescentus, for instance, exhibits a stalk-like extension that carries an adhesive holdfast mediating surface attachment.					
30707707	2	48	theme	adhesive	357:364	arg1	holdfast					366:373	an adhesive holdfast	354:373	an adhesive holdfast mediating surface attachment	354:402	Caulobacter crescentus, for instance, exhibits a stalk-like extension that carries an adhesive holdfast mediating surface attachment.					
30707707	4	49	theme	subcellular	755:765	arg1	domain					767:772	a distinct subcellular domain	744:772	a distinct subcellular domain with specific mechanical properties	744:808	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	6	50	theme	divisome	1150:1157	arg1	components					1132:1141	specific components	1123:1141	specific components of the divisome and elongasome	1123:1172	Our results show that the biosynthesis of stalk peptidoglycan involves a dedicated peptidoglycan biosynthetic complex that combines specific components of the divisome and elongasome, suggesting that the repurposing of preexisting machinery provides a straightforward means to evolve new morphological traits.					
30707707	5	51	theme	biosynthetic	950:961	arg1	machinery					963:971	the cell wall biosynthetic machinery	936:971	the cell wall biosynthetic machinery	936:971	To identify factors that participate in stalk formation, we systematically inactivated and localized predicted components of the cell wall biosynthetic machinery of C. crescentus.					
30707707	1	52	theme	complex	161:167	arg1	shapes					174:179	complex cell shapes	161:179	complex cell shapes	161:179	Many bacteria have complex cell shapes, but the mechanisms producing their distinctive morphologies are still poorly understood.					
30707707	4	53	theme	crosslinks	695:704	arg1	nature					662:667	nature	662:667	nature	662:667	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	4	53	theme	crosslinks	695:704	arg1	proportion					673:682	proportion	673:682	proportion	673:682	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
30707707	5	54	theme	stalk	851:855	arg1	formation					857:865	stalk formation	851:865	stalk formation	851:865	To identify factors that participate in stalk formation, we systematically inactivated and localized predicted components of the cell wall biosynthetic machinery of C. crescentus.					
30707707	1	55	contain	have	156:159	arg1	bacteria					147:154	Many bacteria	142:154	Many bacteria	142:154	Many bacteria have complex cell shapes, but the mechanisms producing their distinctive morphologies are still poorly understood.					
30707707	1	55	contain	have	156:159	arg2	shapes					174:179	complex cell shapes	161:179	complex cell shapes	161:179	Many bacteria have complex cell shapes, but the mechanisms producing their distinctive morphologies are still poorly understood.					
30707707	1	56	theme	cell	169:172	arg1	shapes					174:179	complex cell shapes	161:179	complex cell shapes	161:179	Many bacteria have complex cell shapes, but the mechanisms producing their distinctive morphologies are still poorly understood.					
30707707	4	57	theme	peptide	687:693	arg1	crosslinks					695:704	peptide crosslinks	687:704	peptide crosslinks	687:704	We analyzed the composition of cell body and stalk peptidoglycan and identified significant differences in the nature and proportion of peptide crosslinks, indicating that the stalk represents a distinct subcellular domain with specific mechanical properties.					
29702172	3	0	dep	ratios	342:347	arg1	100:0					350:354	100:0	350:354	100:0	350:354	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	7	1	theme	plant	932:936	arg1	ESNFs					953:957	plant mucilage-based ESNFs	932:957	plant mucilage-based ESNFs	932:957	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	3	2	from	conductivity	464:475	arg1	similar					428:434	similar	428:434	similar	428:434	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	4	3	theme	ESNFs	525:529	arg1	Morphology					507:516	Morphology	507:516	Morphology of the ESNFs	507:529	Morphology of the ESNFs was examined using scanning electron microscopy (SEM).					
29702172	5	4	theme	chemical	711:718	arg1	composition					720:730	chemical composition	711:730	chemical composition	711:730	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	7	5	theme	beans	1068:1072	arg1	better					1077:1082	better	1077:1082	better	1077:1082	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	7	5	theme	beans	1068:1072	arg1	mucilage					1051:1058	the mucilage	1047:1058	the mucilage of chan beans	1047:1072	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	3	6	from	similar	428:434	arg1	viscosity					439:447	viscosity	439:447	viscosity	439:447	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	3	6	from	similar	428:434	arg1	conductivity					464:475	electrical conductivity	453:475	electrical conductivity	453:475	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	7	7	theme	chan	1063:1066	arg1	beans					1068:1072	chan beans	1063:1072	chan beans	1063:1072	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	3	8	theme	different	325:333	arg1	ratios					342:347	different volume ratios	325:347	different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100)	325:393	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	3	9	theme	suitable	477:484	arg1	viscosity					439:447	viscosity	439:447	viscosity	439:447	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	3	10	theme	volume	335:340	arg1	ratios					342:347	different volume ratios	325:347	different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100)	325:393	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	6	11	theme	cell	830:833	arg1	proliferation					835:847	fibroblast cell proliferation	819:847	fibroblast cell proliferation	819:847	The ability of the NFs to support fibroblast cell proliferation was investigated in vitro using the optimized mucilage/PVA solutions.					
29702172	3	12	from	viscosity	439:447	arg1	similar					428:434	similar	428:434	similar	428:434	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	1	13	attach	isolated	148:155	arg1	chan					162:165	chan	162:165	chan	162:165	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	1	13	attach	isolated	148:155	arg2	mucilage					139:146	mucilage	139:146	mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica	139:235	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	1	13	attach	isolated	148:155	arg1	beans					178:182	linaza beans	171:182	linaza beans	171:182	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	3	14	theme	electrical	453:462	arg1	conductivity					464:475	electrical conductivity	453:475	electrical conductivity	453:475	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	5	15	dep	Fourier	586:592	arg1	transform					594:602	transform	594:602	transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies	594:683	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	1	16	theme	linaza	171:176	arg1	beans					178:182	linaza beans	171:182	linaza beans	171:182	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	1	17	theme	Electrospun	89:99	arg1	ESNFs					113:117	ESNFs	113:117	ESNFs	113:117	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	1	17	theme	Electrospun	89:99	arg1	nanofibers					101:110	Electrospun nanofibers	89:110	Electrospun nanofibers (ESNFs)	89:118	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	0	18	theme	plant	12:16	arg1	mucilage					18:25	Electrospun plant mucilage	0:25	Electrospun plant mucilage	0:25	Electrospun plant mucilage nanofibers as biocompatible scaffolds for cell proliferation.					
29702172	7	19	theme	PVA	1038:1040	arg1	ESNFs					1029:1033	ESNFs	1029:1033	ESNFs of PVA	1029:1040	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	2	20	theme	aiding	279:284	arg1	agent					286:290	an aiding agent	276:290	an aiding agent	276:290	Poly(vinyl alcohol) (PVA) was used as an aiding agent.					
29702172	2	20	theme	aiding	279:284	arg1	Poly					238:241	Poly	238:241	Poly(vinyl alcohol) (PVA)	238:262	Poly(vinyl alcohol) (PVA) was used as an aiding agent.					
29702172	6	21	theme	fibroblast	819:828	arg1	proliferation					835:847	fibroblast cell proliferation	819:847	fibroblast cell proliferation	819:847	The ability of the NFs to support fibroblast cell proliferation was investigated in vitro using the optimized mucilage/PVA solutions.					
29702172	0	22	theme	Electrospun	0:10	arg1	mucilage					18:25	Electrospun plant mucilage	0:25	Electrospun plant mucilage	0:25	Electrospun plant mucilage nanofibers as biocompatible scaffolds for cell proliferation.					
29702172	5	23	used	used	690:693	arg2	Fourier					586:592	Fourier	586:592	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies	586:683	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	1	24	theme	mozote	188:193	arg1	stem					195:198	mozote stem	188:198	mozote stem	188:198	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	3	25	theme	ratios	342:347	arg1	solutions					312:320	Mucilage/PVA mixed solutions	293:320	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100)	293:393	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	5	26	theme	differential	637:648	arg1	DSC					672:674	DSC	672:674	DSC	672:674	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	5	26	theme	differential	637:648	arg1	calorimetry					659:669	differential scanning calorimetry	637:669	differential scanning calorimetry (DSC)	637:675	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	5	27	dep	transform	594:602	arg1	infrared					604:611	infrared	604:611	transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies	594:683	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	5	28	theme	scanning	650:657	arg1	DSC					672:674	DSC	672:674	DSC	672:674	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	5	28	theme	scanning	650:657	arg1	calorimetry					659:669	differential scanning calorimetry	637:669	differential scanning calorimetry (DSC)	637:675	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	6	29	theme	NFs	804:806	arg1	ability					789:795	The ability	785:795	The ability of the NFs to support fibroblast cell proliferation	785:847	The ability of the NFs to support fibroblast cell proliferation was investigated in vitro using the optimized mucilage/PVA solutions.					
29702172	0	30	theme	biocompatible	41:53	arg1	scaffolds					55:63	biocompatible scaffolds	41:63	biocompatible scaffolds for cell proliferation	41:86	Electrospun plant mucilage nanofibers as biocompatible scaffolds for cell proliferation.					
29702172	6	31	theme	mucilage/PVA	895:906	arg1	solutions					908:916	the optimized mucilage/PVA solutions	881:916	the optimized mucilage/PVA solutions	881:916	The ability of the NFs to support fibroblast cell proliferation was investigated in vitro using the optimized mucilage/PVA solutions.					
29702172	7	32	theme	better	1017:1022	arg1	growth					995:1000	fibroblast cell growth	979:1000	fibroblast cell growth	979:1000	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	1	33	theme	available	213:221	arg1	beans					178:182	linaza beans	171:182	linaza beans	171:182	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	6	34	theme	optimized	885:893	arg1	solutions					908:916	the optimized mucilage/PVA solutions	881:916	the optimized mucilage/PVA solutions	881:916	The ability of the NFs to support fibroblast cell proliferation was investigated in vitro using the optimized mucilage/PVA solutions.					
29702172	5	35	theme	nanofibers	767:776	arg1	composition					720:730	chemical composition	711:730	chemical composition	711:730	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	5	35	theme	nanofibers	767:776	arg1	characteristics					744:758	thermal characteristics	736:758	thermal characteristics	736:758	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	1	36	from	available	213:221	arg1	Rica					232:235	Rica	232:235	Rica	232:235	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	5	37	theme	thermal	736:742	arg1	characteristics					744:758	thermal characteristics	736:758	thermal characteristics	736:758	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	7	38	theme	cell	1131:1134	arg1	proliferation					1136:1148	cell proliferation	1131:1148	cell proliferation	1131:1148	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	3	39	theme	mixed	306:310	arg1	solutions					312:320	Mucilage/PVA mixed solutions	293:320	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100)	293:393	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	7	40	theme	fibroblast	979:988	arg1	growth					995:1000	fibroblast cell growth	979:1000	fibroblast cell growth	979:1000	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	5	41	dep	spectrometer	613:624	arg1	studies					677:683	studies	677:683	studies	677:683	Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC) studies were used to characterize chemical composition and thermal characteristics of the nanofibers (NFs).					
29702172	2	42	used	used	268:271	arg2	agent					286:290	an aiding agent	276:290	an aiding agent	276:290	Poly(vinyl alcohol) (PVA) was used as an aiding agent.					
29702172	2	42	used	used	268:271	arg2	alcohol					249:255	vinyl alcohol	243:255	vinyl alcohol	243:255	Poly(vinyl alcohol) (PVA) was used as an aiding agent.					
29702172	2	42	used	used	268:271	arg2	Poly					238:241	Poly	238:241	Poly(vinyl alcohol) (PVA)	238:262	Poly(vinyl alcohol) (PVA) was used as an aiding agent.					
29702172	2	42	used	used	268:271	arg2	PVA					259:261	PVA	259:261	PVA	259:261	Poly(vinyl alcohol) (PVA) was used as an aiding agent.					
29702172	7	43	theme	cell	990:993	arg1	growth					995:1000	fibroblast cell growth	979:1000	fibroblast cell growth	979:1000	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	4	44	theme	scanning	550:557	arg1	SEM					580:582	SEM	580:582	SEM	580:582	Morphology of the ESNFs was examined using scanning electron microscopy (SEM).					
29702172	4	44	theme	scanning	550:557	arg1	microscopy					568:577	scanning electron microscopy	550:577	scanning electron microscopy (SEM)	550:583	Morphology of the ESNFs was examined using scanning electron microscopy (SEM).					
29702172	1	45	from	Rica	232:235	arg1	available					213:221	available	213:221	available	213:221	Electrospun nanofibers (ESNFs) were prepared from mucilage isolated from chan and linaza beans and mozote stem commercially available in Costa Rica.					
29702172	7	46	theme	mucilage-based	938:951	arg1	ESNFs					953:957	plant mucilage-based ESNFs	932:957	plant mucilage-based ESNFs	932:957	Results show plant mucilage-based ESNFs are well-suited for fibroblast cell growth, significantly better than ESNFs of PVA; and the mucilage of chan beans is better than those of mozote and linaza for supporting cell proliferation.					
29702172	3	47	theme	Mucilage/PVA	293:304	arg1	solutions					312:320	Mucilage/PVA mixed solutions	293:320	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100)	293:393	Mucilage/PVA mixed solutions of different volume ratios (100:0, 80:20, 60:40, 40:60, 20:80 and 0:100) were prepared and adjusted to be similar in viscosity and electrical conductivity suitable for electrospinning.					
29702172	2	48	theme	vinyl	243:247	arg1	Poly					238:241	Poly	238:241	Poly(vinyl alcohol) (PVA)	238:262	Poly(vinyl alcohol) (PVA) was used as an aiding agent.					
29702172	2	48	theme	vinyl	243:247	arg1	alcohol					249:255	vinyl alcohol	243:255	vinyl alcohol	243:255	Poly(vinyl alcohol) (PVA) was used as an aiding agent.					
29702172	0	49	theme	cell	69:72	arg1	proliferation					74:86	cell proliferation	69:86	cell proliferation	69:86	Electrospun plant mucilage nanofibers as biocompatible scaffolds for cell proliferation.					
29702172	4	50	theme	electron	559:566	arg1	SEM					580:582	SEM	580:582	SEM	580:582	Morphology of the ESNFs was examined using scanning electron microscopy (SEM).					
29702172	4	50	theme	electron	559:566	arg1	microscopy					568:577	scanning electron microscopy	550:577	scanning electron microscopy (SEM)	550:583	Morphology of the ESNFs was examined using scanning electron microscopy (SEM).					
30241825	3	0	theme	oil	772:774	arg1	phase					776:780	oil phase	772:780	oil phase with medium chain triglyceride (MCT) and Tween 80	772:830	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	1	1	from	stability	378:386	arg1	water					411:415	water	411:415	water	411:415	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	10	2	theme	normal	1775:1780	arg1	cells					1782:1786	normal cells	1775:1786	normal cells	1775:1786	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	1	3	contain	have	193:196	arg1	curcumin					184:191	curcumin	184:191	curcumin	184:191	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	3	contain	have	193:196	arg1	Coumarin					171:178	Coumarin	171:178	Coumarin	171:178	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	3	contain	have	193:196	arg2	spectrum					205:212	a wide spectrum	198:212	a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer	198:327	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	7	4	theme	encapsulated	1170:1181	arg1	PEs					1183:1185	encapsulated PEs	1170:1185	encapsulated PEs	1170:1185	Release kinetic profiles for encapsulated PEs displayed sustained release with supposed increase bioavailability.					
30241825	8	5	theme	Higher	1255:1260	arg1	percent					1270:1276	Higher release percent	1255:1276	Higher release percent	1255:1276	Higher release percent were detected for curcumin encapsulated PE in contrast to coumarin.					
30241825	3	6	theme	chain	794:798	arg1	triglyceride					800:811	medium chain triglyceride	787:811	medium chain triglyceride (MCT)	787:817	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	3	6	theme	chain	794:798	arg1	MCT					814:816	MCT	814:816	MCT	814:816	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	1	7	theme	wide	200:203	arg1	spectrum					205:212	a wide spectrum	198:212	a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer	198:327	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	4	8	with	nanoemulsions	848:860	arg1	sizes					892:896	average particle sizes	875:896	average particle sizes (≤150 nm)	875:906	The fabricated nanoemulsions and PEs with average particle sizes (≤150 nm) were obtained.					
30241825	4	8	with	nanoemulsions	848:860	arg1	≤150 nm					899:905	≤150 nm	899:905	≤150 nm	899:905	The fabricated nanoemulsions and PEs with average particle sizes (≤150 nm) were obtained.					
30241825	7	9	theme	Release	1141:1147	arg1	profiles					1157:1164	Release kinetic profiles	1141:1164	Release kinetic profiles for encapsulated PEs	1141:1185	Release kinetic profiles for encapsulated PEs displayed sustained release with supposed increase bioavailability.					
30241825	3	10	theme	nanocellulose	628:640	arg1	particles					648:656	aminated nanocellulose (ANC) particles	619:656	aminated nanocellulose (ANC) particles	619:656	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	7	11	theme	increase	1229:1236	arg1	bioavailability					1238:1252	supposed increase bioavailability	1220:1252	supposed increase bioavailability	1220:1252	Release kinetic profiles for encapsulated PEs displayed sustained release with supposed increase bioavailability.					
30241825	1	12	theme	activities	248:257	arg1	spectrum					205:212	a wide spectrum	198:212	a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer	198:327	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	10	13	theme	promising	1662:1670	arg1	curcumin					1650:1657	curcumin	1650:1657	curcumin	1650:1657	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	10	13	theme	promising	1662:1670	arg1	candidates					1672:1681	promising candidates	1662:1681	promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells	1662:1786	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	10	13	theme	promising	1662:1670	arg1	coumarin					1637:1644	PE coumarin	1634:1644	PE coumarin	1634:1644	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	3	14	theme	factorial	688:696	arg1	design					711:716	a full factorial optimization design	681:716	a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80	681:830	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	0	15	theme	curcumin	83:90	arg1	activities					159:168	anticancer and antimicrobial activities	130:168	anticancer and antimicrobial activities	130:168	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	0	15	theme	curcumin	83:90	arg1	nanoencapsulations					92:109	coumarin and curcumin nanoencapsulations	70:109	coumarin and curcumin nanoencapsulations	70:109	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	9	16	theme	coumarin	1383:1390	arg1	PEs					1412:1414	coumarin and curcumin loaded PEs	1383:1414	coumarin and curcumin loaded PEs	1383:1414	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	5	17	theme	storage	970:976	arg1	time					978:981	storage time	970:981	storage time	970:981	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	6	18	theme	Encapsulation	1061:1073	arg1	EE					1087:1088	EE	1087:1088	EE	1087:1088	Encapsulation efficiency (EE) of coumarin and curcumin were determined as >90%.					
30241825	6	18	theme	Encapsulation	1061:1073	arg1	efficiency					1075:1084	Encapsulation efficiency	1061:1084	Encapsulation efficiency (EE) of coumarin and curcumin	1061:1114	Encapsulation efficiency (EE) of coumarin and curcumin were determined as >90%.					
30241825	6	18	theme	Encapsulation	1061:1073	arg1	%					1138:1138	>90%	1135:1138	>90%	1135:1138	Encapsulation efficiency (EE) of coumarin and curcumin were determined as >90%.					
30241825	4	19	with	PEs	866:868	arg1	sizes					892:896	average particle sizes	875:896	average particle sizes (≤150 nm)	875:906	The fabricated nanoemulsions and PEs with average particle sizes (≤150 nm) were obtained.					
30241825	4	19	with	PEs	866:868	arg1	≤150 nm					899:905	≤150 nm	899:905	≤150 nm	899:905	The fabricated nanoemulsions and PEs with average particle sizes (≤150 nm) were obtained.					
30241825	9	20	theme	curcumin	1396:1403	arg1	PEs					1412:1414	coumarin and curcumin loaded PEs	1383:1414	coumarin and curcumin loaded PEs	1383:1414	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	2	21	theme	current	444:450	arg1	study					452:456	the current study	440:456	the current study	440:456	The main objective of the current study was to overcome these drawbacks via improved bioavailability by nanoencapsulated emulsions.					
30241825	5	22	from	pH	987:988	arg1	stability					997:1005	the stability	993:1005	the stability of emulsions	993:1018	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	5	23	theme	zeta	1044:1047	arg1	potentials					1049:1058	zeta potentials	1044:1058	zeta potentials	1044:1058	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	2	24	theme	improved	494:501	arg1	bioavailability					503:517	improved bioavailability	494:517	improved bioavailability by nanoencapsulated emulsions	494:547	The main objective of the current study was to overcome these drawbacks via improved bioavailability by nanoencapsulated emulsions.					
30241825	0	25	theme	Pickering	0:8	arg1	emulsions					10:18	Pickering emulsions	0:18	Pickering emulsions	0:18	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	5	26	from	time	978:981	arg1	stability					997:1005	the stability	993:1005	the stability of emulsions	993:1018	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	1	27	theme	therapeutic	342:352	arg1	applications					354:365	therapeutic applications	342:365	therapeutic applications	342:365	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	5	28	theme	emulsions	1010:1018	arg1	stability					997:1005	the stability	993:1005	the stability of emulsions	993:1018	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	6	29	theme	curcumin	1107:1114	arg1	EE					1087:1088	EE	1087:1088	EE	1087:1088	Encapsulation efficiency (EE) of coumarin and curcumin were determined as >90%.					
30241825	6	29	theme	curcumin	1107:1114	arg1	efficiency					1075:1084	Encapsulation efficiency	1061:1084	Encapsulation efficiency (EE) of coumarin and curcumin	1061:1114	Encapsulation efficiency (EE) of coumarin and curcumin were determined as >90%.					
30241825	6	29	theme	curcumin	1107:1114	arg1	%					1138:1138	>90%	1135:1138	>90%	1135:1138	Encapsulation efficiency (EE) of coumarin and curcumin were determined as >90%.					
30241825	9	30	dep	microorganisms	1540:1553	arg1	microorganisms					1540:1553	different microorganisms	1530:1553	different microorganisms (Gram (+), Gram (-) and fungi)	1530:1584	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	30	dep	microorganisms	1540:1553	arg1	fungi					1579:1583	fungi	1579:1583	fungi	1579:1583	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	30	dep	microorganisms	1540:1553	arg1	Gram					1566:1569	Gram	1566:1569	Gram	1566:1569	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	30	dep	microorganisms	1540:1553	arg1	Gram					1556:1559	Gram	1556:1559	Gram	1556:1559	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	10	31	theme	preferential	1726:1737	arg1	killing					1739:1745	preferential killing	1726:1745	preferential killing of cancer cells compared to normal cells	1726:1786	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	7	32	theme	supposed	1220:1227	arg1	bioavailability					1238:1252	supposed increase bioavailability	1220:1252	supposed increase bioavailability	1220:1252	Release kinetic profiles for encapsulated PEs displayed sustained release with supposed increase bioavailability.					
30241825	6	33	theme	coumarin	1094:1101	arg1	EE					1087:1088	EE	1087:1088	EE	1087:1088	Encapsulation efficiency (EE) of coumarin and curcumin were determined as >90%.					
30241825	6	33	theme	coumarin	1094:1101	arg1	efficiency					1075:1084	Encapsulation efficiency	1061:1084	Encapsulation efficiency (EE) of coumarin and curcumin	1061:1114	Encapsulation efficiency (EE) of coumarin and curcumin were determined as >90%.					
30241825	6	33	theme	coumarin	1094:1101	arg1	%					1138:1138	>90%	1135:1138	>90%	1135:1138	Encapsulation efficiency (EE) of coumarin and curcumin were determined as >90%.					
30241825	3	34	theme	design	711:716	arg1	application					666:676	application	666:676	application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80	666:830	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	0	35	theme	coumarin	70:77	arg1	activities					159:168	anticancer and antimicrobial activities	130:168	anticancer and antimicrobial activities	130:168	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	0	35	theme	coumarin	70:77	arg1	nanoencapsulations					92:109	coumarin and curcumin nanoencapsulations	70:109	coumarin and curcumin nanoencapsulations	70:109	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	1	36	theme	low	374:376	arg1	stability					378:386	low stability	374:386	low stability	374:386	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	10	37	theme	cells	1757:1761	arg1	killing					1739:1745	preferential killing	1726:1745	preferential killing of cancer cells compared to normal cells	1726:1786	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	9	38	theme	human	1492:1496	arg1	lines					1503:1507	human cell lines	1492:1507	human cell lines (L929 and MCF-7)	1492:1524	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	38	theme	human	1492:1496	arg1	MCF-7					1519:1523	MCF-7	1519:1523	MCF-7	1519:1523	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	38	theme	human	1492:1496	arg1	L929					1510:1513	L929	1510:1513	L929	1510:1513	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	3	39	theme	different	747:755	arg1	composition					757:767	different composition	747:767	different composition of oil phase with medium chain triglyceride (MCT) and Tween 80	747:830	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	4	40	theme	average	875:881	arg1	sizes					892:896	average particle sizes	875:896	average particle sizes (≤150 nm)	875:906	The fabricated nanoemulsions and PEs with average particle sizes (≤150 nm) were obtained.					
30241825	4	40	theme	average	875:881	arg1	≤150 nm					899:905	≤150 nm	899:905	≤150 nm	899:905	The fabricated nanoemulsions and PEs with average particle sizes (≤150 nm) were obtained.					
30241825	2	41	theme	main	422:425	arg1	objective					427:435	The main objective	418:435	The main objective of the current study	418:456	The main objective of the current study was to overcome these drawbacks via improved bioavailability by nanoencapsulated emulsions.					
30241825	9	42	theme	different	1530:1538	arg1	microorganisms					1540:1553	different microorganisms	1530:1553	different microorganisms (Gram (+), Gram (-) and fungi)	1530:1584	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	42	theme	different	1530:1538	arg1	fungi					1579:1583	fungi	1579:1583	fungi	1579:1583	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	42	theme	different	1530:1538	arg1	Gram					1556:1559	Gram	1556:1559	Gram	1556:1559	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	3	43	theme	medium	787:792	arg1	triglyceride					800:811	medium chain triglyceride	787:811	medium chain triglyceride (MCT)	787:817	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	3	43	theme	medium	787:792	arg1	MCT					814:816	MCT	814:816	MCT	814:816	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	10	44	dep	candidates	1672:1681	arg1	prevent					1718:1724	prevent	1718:1724	to prevent preferential killing of cancer cells compared to normal cells	1715:1786	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	10	44	dep	candidates	1672:1681	arg1	inhibit					1686:1692	inhibit	1686:1692	to inhibit microbial growth	1683:1709	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	3	45	with	phase	776:780	arg1	Tween					823:827	Tween	823:827	Tween	823:827	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	3	45	with	phase	776:780	arg1	triglyceride					800:811	medium chain triglyceride	787:811	medium chain triglyceride (MCT)	787:817	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	3	45	with	phase	776:780	arg1	MCT					814:816	MCT	814:816	MCT	814:816	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	10	46	theme	PE	1634:1635	arg1	curcumin					1650:1657	curcumin	1650:1657	curcumin	1650:1657	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	10	46	theme	PE	1634:1635	arg1	candidates					1672:1681	promising candidates	1662:1681	promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells	1662:1786	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	10	46	theme	PE	1634:1635	arg1	coumarin					1637:1644	PE coumarin	1634:1644	PE coumarin	1634:1644	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	3	47	theme	phase	776:780	arg1	composition					757:767	different composition	747:767	different composition of oil phase with medium chain triglyceride (MCT) and Tween 80	747:830	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	9	48	dep	In	1346:1347	arg1	vitro					1349:1353	vitro	1349:1353	vitro	1349:1353	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	7	49	theme	kinetic	1149:1155	arg1	profiles					1157:1164	Release kinetic profiles	1141:1164	Release kinetic profiles for encapsulated PEs	1141:1185	Release kinetic profiles for encapsulated PEs displayed sustained release with supposed increase bioavailability.					
30241825	5	50	theme	Influencing	923:933	arg1	factors					935:941	Influencing factors	923:941	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions	923:1018	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	5	50	theme	Influencing	923:933	arg1	pH					987:988	pH	987:988	pH on the stability of emulsions	987:1018	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	5	50	theme	Influencing	923:933	arg1	concentration					955:967	ANC concentration	951:967	ANC concentration	951:967	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	5	50	theme	Influencing	923:933	arg1	time					978:981	storage time	970:981	storage time	970:981	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	3	51	contain	containing	736:745	arg1	nanoemulsions					722:734	nanoemulsions	722:734	nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80	722:830	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	3	51	contain	containing	736:745	arg2	composition					757:767	different composition	747:767	different composition of oil phase with medium chain triglyceride (MCT) and Tween 80	747:830	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	8	52	theme	release	1262:1268	arg1	percent					1270:1276	Higher release percent	1255:1276	Higher release percent	1255:1276	Higher release percent were detected for curcumin encapsulated PE in contrast to coumarin.					
30241825	0	53	theme	antimicrobial	145:157	arg1	activities					159:168	anticancer and antimicrobial activities	130:168	anticancer and antimicrobial activities	130:168	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	0	53	theme	antimicrobial	145:157	arg1	nanoencapsulations					92:109	coumarin and curcumin nanoencapsulations	70:109	coumarin and curcumin nanoencapsulations	70:109	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	3	54	theme	aminated	619:626	arg1	nanocellulose					628:640	aminated nanocellulose	619:640	aminated nanocellulose (ANC) particles	619:656	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	3	54	theme	aminated	619:626	arg1	ANC					643:645	ANC	643:645	ANC	643:645	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	1	55	theme	biological	217:226	arg1	antimicrobial					300:312	antimicrobial	300:312	antimicrobial	300:312	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	55	theme	biological	217:226	arg1	anti-inflammatory					282:297	anti-inflammatory	282:297	anti-inflammatory	282:297	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	55	theme	biological	217:226	arg1	antioxidant					269:279	antioxidant	269:279	antioxidant	269:279	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	55	theme	biological	217:226	arg1	anticancer					318:327	anticancer	318:327	anticancer	318:327	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	55	theme	biological	217:226	arg1	activities					248:257	biological and pharmacological activities	217:257	biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer	217:327	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	9	56	dep	lines	1503:1507	arg1	lines					1503:1507	human cell lines	1492:1507	human cell lines (L929 and MCF-7)	1492:1524	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	56	dep	lines	1503:1507	arg1	MCF-7					1519:1523	MCF-7	1519:1523	MCF-7	1519:1523	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	56	dep	lines	1503:1507	arg1	L929					1510:1513	L929	1510:1513	L929	1510:1513	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	1	57	theme	pharmacological	232:246	arg1	antimicrobial					300:312	antimicrobial	300:312	antimicrobial	300:312	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	57	theme	pharmacological	232:246	arg1	anti-inflammatory					282:297	anti-inflammatory	282:297	anti-inflammatory	282:297	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	57	theme	pharmacological	232:246	arg1	antioxidant					269:279	antioxidant	269:279	antioxidant	269:279	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	57	theme	pharmacological	232:246	arg1	anticancer					318:327	anticancer	318:327	anticancer	318:327	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	1	57	theme	pharmacological	232:246	arg1	activities					248:257	biological and pharmacological activities	217:257	biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer	217:327	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	8	58	theme	encapsulated	1305:1316	arg1	PE					1318:1319	curcumin encapsulated PE	1296:1319	curcumin encapsulated PE	1296:1319	Higher release percent were detected for curcumin encapsulated PE in contrast to coumarin.					
30241825	9	59	theme	In	1346:1347	arg1	evaluation					1368:1377	In vitro cytotoxicity evaluation	1346:1377	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs	1346:1414	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	3	60	theme	full	683:686	arg1	design					711:716	a full factorial optimization design	681:716	a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80	681:830	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	9	61	theme	cytotoxicity	1355:1366	arg1	evaluation					1368:1377	In vitro cytotoxicity evaluation	1346:1377	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs	1346:1414	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	5	62	theme	ANC	951:953	arg1	concentration					955:967	ANC concentration	951:967	ANC concentration	951:967	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	0	63	theme	In	112:113	arg1	release					121:127	In vitro release	112:127	In vitro release	112:127	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	1	64	theme	poor	392:395	arg1	solubility					397:406	poor solubility	392:406	poor solubility	392:406	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	3	65	theme	Pickering	550:558	arg1	emulsion					560:567	Pickering emulsion	550:567	Pickering emulsion (PE) via oil-in-water approach	550:598	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	3	65	theme	Pickering	550:558	arg1	PE					570:571	PE	570:571	PE	570:571	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	0	66	theme	anticancer	130:139	arg1	activities					159:168	anticancer and antimicrobial activities	130:168	anticancer and antimicrobial activities	130:168	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	0	66	theme	anticancer	130:139	arg1	nanoencapsulations					92:109	coumarin and curcumin nanoencapsulations	70:109	coumarin and curcumin nanoencapsulations	70:109	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	9	67	theme	loaded	1405:1410	arg1	PEs					1412:1414	coumarin and curcumin loaded PEs	1383:1414	coumarin and curcumin loaded PEs	1383:1414	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	5	68	from	concentration	955:967	arg1	stability					997:1005	the stability	993:1005	the stability of emulsions	993:1018	Influencing factors such as ANC concentration, storage time and pH on the stability of emulsions were examined alongside zeta potentials.					
30241825	4	69	theme	fabricated	837:846	arg1	nanoemulsions					848:860	The fabricated nanoemulsions	833:860	The fabricated nanoemulsions	833:860	The fabricated nanoemulsions and PEs with average particle sizes (≤150 nm) were obtained.					
30241825	0	70	dep	In	112:113	arg1	vitro					115:119	vitro	115:119	vitro	115:119	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	10	71	theme	microbial	1694:1702	arg1	growth					1704:1709	microbial growth	1694:1709	microbial growth	1694:1709	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	0	72	theme	nanocellulosic-based	31:50	arg1	nanoparticles					52:64	nanocellulosic-based nanoparticles	31:64	nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities	31:168	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	0	73	dep	nanoencapsulations	92:109	arg1	release					121:127	In vitro release	112:127	In vitro release	112:127	Pickering emulsions stabilized nanocellulosic-based nanoparticles for coumarin and curcumin nanoencapsulations: In vitro release, anticancer and antimicrobial activities.					
30241825	1	74	from	solubility	397:406	arg1	water					411:415	water	411:415	water	411:415	Coumarin and curcumin have a wide spectrum of biological and pharmacological activities including antioxidant, anti-inflammatory, antimicrobial and anticancer but hindered therapeutic applications due to low stability and poor solubility in water.					
30241825	9	75	theme	anticancer	1446:1455	arg1	activities					1475:1484	anticancer and antimicrobial activities	1446:1484	anticancer and antimicrobial activities	1446:1484	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	2	76	theme	nanoencapsulated	522:537	arg1	emulsions					539:547	nanoencapsulated emulsions	522:547	nanoencapsulated emulsions	522:547	The main objective of the current study was to overcome these drawbacks via improved bioavailability by nanoencapsulated emulsions.					
30241825	3	77	theme	optimization	698:709	arg1	design					711:716	a full factorial optimization design	681:716	a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80	681:830	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
30241825	4	78	theme	particle	883:890	arg1	sizes					892:896	average particle sizes	875:896	average particle sizes (≤150 nm)	875:906	The fabricated nanoemulsions and PEs with average particle sizes (≤150 nm) were obtained.					
30241825	4	78	theme	particle	883:890	arg1	≤150 nm					899:905	≤150 nm	899:905	≤150 nm	899:905	The fabricated nanoemulsions and PEs with average particle sizes (≤150 nm) were obtained.					
30241825	2	79	theme	study	452:456	arg1	objective					427:435	The main objective	418:435	The main objective of the current study	418:456	The main objective of the current study was to overcome these drawbacks via improved bioavailability by nanoencapsulated emulsions.					
30241825	7	80	theme	sustained	1197:1205	arg1	release					1207:1213	sustained release	1197:1213	sustained release	1197:1213	Release kinetic profiles for encapsulated PEs displayed sustained release with supposed increase bioavailability.					
30241825	9	81	theme	antimicrobial	1461:1473	arg1	activities					1475:1484	anticancer and antimicrobial activities	1446:1484	anticancer and antimicrobial activities	1446:1484	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	8	82	located	detected	1283:1290	arg1	contrast					1324:1331	contrast	1324:1331	contrast to coumarin	1324:1343	Higher release percent were detected for curcumin encapsulated PE in contrast to coumarin.					
30241825	8	82	located	detected	1283:1290	arg2	percent					1270:1276	Higher release percent	1255:1276	Higher release percent	1255:1276	Higher release percent were detected for curcumin encapsulated PE in contrast to coumarin.					
30241825	10	83	theme	cancer	1750:1755	arg1	cells					1757:1761	cancer cells	1750:1761	cancer cells	1750:1761	The results clearly demonstrated PE coumarin and curcumin as promising candidates to inhibit microbial growth and to prevent preferential killing of cancer cells compared to normal cells.					
30241825	9	84	theme	cell	1498:1501	arg1	lines					1503:1507	human cell lines	1492:1507	human cell lines (L929 and MCF-7)	1492:1524	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	84	theme	cell	1498:1501	arg1	MCF-7					1519:1523	MCF-7	1519:1523	MCF-7	1519:1523	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	9	84	theme	cell	1498:1501	arg1	L929					1510:1513	L929	1510:1513	L929	1510:1513	In vitro cytotoxicity evaluation for coumarin and curcumin loaded PEs were further investigated for anticancer and antimicrobial activities using human cell lines (L929 and MCF-7) and different microorganisms (Gram (+), Gram (-) and fungi), respectively.					
30241825	3	85	theme	oil-in-water	578:589	arg1	approach					591:598	oil-in-water approach	578:598	oil-in-water approach	578:598	Pickering emulsion (PE) via oil-in-water approach were stabilized by aminated nanocellulose (ANC) particles through application of a full factorial optimization design for nanoemulsions containing different composition of oil phase with medium chain triglyceride (MCT) and Tween 80.					
31882640	0	0	theme	PMEI	77:80	arg1	families					87:94	the PME and PMEI gene families	65:94	families	87:94	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	6	1	theme	other	1029:1033	arg1	tissues					1035:1041	other tissues	1029:1041	other tissues from the NimbleGen maize microarray data	1029:1082	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	5	2	theme	pectin	855:860	arg1	process					872:878	pectin catabolic process	855:878	pectin catabolic process	855:878	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	5	3	theme	pectinesterase	926:939	arg1	activities					951:960	pectinesterase inhibitor activities	926:960	pectinesterase inhibitor activities	926:960	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	0	4	from	identification	12:25	arg1	maize					99:103	maize	99:103	maize	99:103	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	6	5	theme	anther	1228:1233	arg1	development					1246:1256	anther and pollen development	1228:1256	development	1246:1256	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	1	6	theme	cell	139:142	arg1	walls					144:148	cell walls	139:148	cell walls in plants	139:158	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	1	7	theme	methyl-esterified	214:230	arg1	polymers					232:239	highly methyl-esterified polymers	207:239	highly methyl-esterified polymers	207:239	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	1	7	theme	methyl-esterified	214:230	arg1	Pectins					106:112	Pectins	106:112	Pectins	106:112	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	0	8	theme	families	87:94	arg1	phylogeny					28:36	phylogeny	28:36	phylogeny	28:36	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	0	8	theme	families	87:94	arg1	analysis					53:60	expression analysis	42:60	expression analysis	42:60	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	0	8	theme	families	87:94	arg1	identification					12:25	Genome-wide identification	0:25	Genome-wide identification	0:25	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	5	9	with	modification	838:849	arg1	functions					895:903	molecular functions	885:903	molecular functions of pectinesterase or pectinesterase inhibitor activities	885:960	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	4	10	theme	ZmPME/PMEI	719:728	arg1	genes					730:734	the closely related ZmPME/PMEI genes	699:734	the closely related ZmPME/PMEI genes	699:734	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	4	11	theme	common	598:603	arg1	compositions					611:622	common motif compositions	598:622	common motif compositions	598:622	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	4	12	theme	same	538:541	arg1	pairs					554:558	the same paralogous pairs	534:558	the same paralogous pairs	534:558	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	0	13	theme	gene	82:85	arg1	families					87:94	the PME and PMEI gene families	65:94	families	87:94	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	4	14	theme	related	711:717	arg1	genes					730:734	the closely related ZmPME/PMEI genes	699:734	the closely related ZmPME/PMEI genes	699:734	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	4	15	theme	functional	669:678	arg1	similarity					680:689	functional similarity	669:689	functional similarity between the closely related ZmPME/PMEI genes	669:734	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	0	16	from	analysis	53:60	arg1	maize					99:103	maize	99:103	maize	99:103	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	5	17	theme	ZmPME/PMEI	795:804	arg1	genes					806:810	the ZmPME/PMEI genes	791:810	the ZmPME/PMEI genes	791:810	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	1	18	from	components	125:134	arg1	plants					153:158	plants	153:158	plants	153:158	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	5	19	theme	molecular	885:893	arg1	functions					895:903	molecular functions	885:903	molecular functions of pectinesterase or pectinesterase inhibitor activities	885:960	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	1	20	theme	walls	144:148	arg1	components					125:134	the major components	115:134	the major components of cell walls in plants	115:158	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	1	20	theme	walls	144:148	arg1	Pectins					106:112	Pectins	106:112	Pectins	106:112	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	4	21	from	members	523:529	arg1	subgroup					575:582	the same subgroup	566:582	the same subgroup	566:582	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	4	21	from	members	523:529	arg1	pairs					554:558	the same paralogous pairs	534:558	the same paralogous pairs	534:558	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	5	22	theme	catabolic	862:870	arg1	process					872:878	pectin catabolic process	855:878	pectin catabolic process	855:878	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	5	23	theme	Gene	737:740	arg1	annotation					751:760	Gene ontology annotation	737:760	Gene ontology annotation analysis	737:769	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	4	24	theme	paralogous	543:552	arg1	pairs					554:558	the same paralogous pairs	534:558	the same paralogous pairs	534:558	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	6	25	theme	microarray	1068:1077	arg1	data					1079:1082	the NimbleGen maize microarray data	1048:1082	the NimbleGen maize microarray data	1048:1082	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	6	26	theme	NimbleGen	1052:1060	arg1	data					1079:1082	the NimbleGen maize microarray data	1048:1082	the NimbleGen maize microarray data	1048:1082	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	5	27	with	process	872:878	arg1	functions					895:903	molecular functions	885:903	molecular functions of pectinesterase or pectinesterase inhibitor activities	885:960	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	7	28	theme	sterile	1372:1378	arg1	anthers					1380:1386	sterile anthers	1372:1386	sterile anthers	1372:1386	In addition, these ZmPME/PMEI genes were highly expressed in the fertile anthers, while lowly or no expressed in sterile anthers.					
31882640	5	29	theme	cell	828:831	arg1	modification					838:849	cell wall modification	828:849	cell wall modification	828:849	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	0	30	theme	Genome-wide	0:10	arg1	identification					12:25	Genome-wide identification	0:25	Genome-wide identification	0:25	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	6	31	theme	pollen	1239:1244	arg1	development					1246:1256	anther and pollen development	1228:1256	development	1246:1256	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	5	32	theme	pectinesterase	908:921	arg1	functions					895:903	molecular functions	885:903	molecular functions of pectinesterase or pectinesterase inhibitor activities	885:960	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	2	33	theme	methylesterase	347:360	arg1	PMEIs					374:378	PMEIs	374:378	PMEIs	374:378	The PMEs are spatially regulated by pectin methylesterase inhibitors (PMEIs).					
31882640	2	33	theme	methylesterase	347:360	arg1	inhibitors					362:371	pectin methylesterase inhibitors	340:371	pectin methylesterase inhibitors (PMEIs)	340:379	The PMEs are spatially regulated by pectin methylesterase inhibitors (PMEIs).					
31882640	4	34	theme	motif	494:498	arg1	analysis					500:507	motif analysis	494:507	motif analysis	494:507	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	3	35	theme	PME	416:418	arg1	genes					429:433	43 and 49 putative PME and PMEI genes	397:433	43 and 49 putative PME and PMEI genes	397:433	In this study, 43 and 49 putative PME and PMEI genes were identified in maize, respectively.					
31882640	6	36	contain	had	1216:1218	arg2	role					1220:1223	role	1220:1223	role	1220:1223	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	6	36	contain	had	1216:1218	arg1	they					1202:1205	they	1202:1205	they	1202:1205	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	4	37	theme	structure	633:641	arg1	patterns					643:650	gene structure patterns	628:650	gene structure patterns	628:650	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	1	38	theme	major	119:123	arg1	components					125:134	the major components	115:134	the major components of cell walls in plants	115:158	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	1	38	theme	major	119:123	arg1	Pectins					106:112	Pectins	106:112	Pectins	106:112	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	0	39	theme	expression	42:51	arg1	analysis					53:60	expression analysis	42:60	expression analysis	42:60	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	1	40	theme	pectin	273:278	arg1	PMEs					297:300	PMEs	297:300	PMEs	297:300	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	1	40	theme	pectin	273:278	arg1	methylesterases					280:294	pectin methylesterases	273:294	pectin methylesterases (PMEs)	273:301	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	4	41	theme	motif	605:609	arg1	compositions					611:622	common motif compositions	598:622	common motif compositions	598:622	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	4	42	theme	gene	628:631	arg1	patterns					643:650	gene structure patterns	628:650	gene structure patterns	628:650	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	4	43	theme	Gene	475:478	arg1	structure					480:488	Gene structure	475:488	Gene structure	475:488	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	5	44	theme	ontology	742:749	arg1	annotation					751:760	Gene ontology annotation	737:760	Gene ontology annotation analysis	737:769	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	6	45	theme	ZmPME/PMEI	976:985	arg1	genes					987:991	35 ZmPME/PMEI genes	973:991	35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data	973:1082	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	6	46	theme	maize	1062:1066	arg1	data					1079:1082	the NimbleGen maize microarray data	1048:1082	the NimbleGen maize microarray data	1048:1082	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	3	47	theme	putative	407:414	arg1	genes					429:433	43 and 49 putative PME and PMEI genes	397:433	43 and 49 putative PME and PMEI genes	397:433	In this study, 43 and 49 putative PME and PMEI genes were identified in maize, respectively.					
31882640	5	48	theme	annotation	751:760	arg1	analysis					762:769	Gene ontology annotation analysis	737:769	Gene ontology annotation analysis	737:769	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	5	49	theme	activities	951:960	arg1	functions					895:903	molecular functions	885:903	molecular functions of pectinesterase or pectinesterase inhibitor activities	885:960	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	6	50	theme	semiq-RT-PCR	1093:1104	arg1	assay					1106:1110	the semiq-RT-PCR assay	1089:1110	the semiq-RT-PCR assay	1089:1110	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	8	51	theme	anther	1464:1469	arg1	development					1449:1459	the development	1445:1459	the development of anther and pollen	1445:1480	This further indicated these genes might be involved in the development of anther and pollen.					
31882640	7	52	theme	fertile	1324:1330	arg1	anthers					1332:1338	the fertile anthers	1320:1338	the fertile anthers	1320:1338	In addition, these ZmPME/PMEI genes were highly expressed in the fertile anthers, while lowly or no expressed in sterile anthers.					
31882640	3	53	theme	PMEI	424:427	arg1	genes					429:433	43 and 49 putative PME and PMEI genes	397:433	43 and 49 putative PME and PMEI genes	397:433	In this study, 43 and 49 putative PME and PMEI genes were identified in maize, respectively.					
31882640	5	54	theme	wall	833:836	arg1	modification					838:849	cell wall modification	828:849	cell wall modification	828:849	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	4	55	contain	had	594:596	arg2	compositions					611:622	common motif compositions	598:622	common motif compositions	598:622	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	4	55	contain	had	594:596	arg1	members					523:529	members	523:529	members in the same paralogous pairs or in the same subgroup	523:582	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	4	55	contain	had	594:596	arg2	patterns					643:650	gene structure patterns	628:650	gene structure patterns	628:650	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	5	56	theme	inhibitor	941:949	arg1	activities					951:960	pectinesterase inhibitor activities	926:960	pectinesterase inhibitor activities	926:960	Gene ontology annotation analysis showed that most of the ZmPME/PMEI genes are involved in cell wall modification and pectin catabolic process with molecular functions of pectinesterase or pectinesterase inhibitor activities.					
31882640	1	57	from	plants	153:158	arg1	components					125:134	the major components	115:134	the major components of cell walls in plants	115:158	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	1	57	from	plants	153:158	arg1	Pectins					106:112	Pectins	106:112	Pectins	106:112	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	1	58	theme	cell	193:196	arg1	walls					198:202	cell walls	193:202	cell walls	193:202	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	0	59	theme	PME	69:71	arg1	phylogeny					28:36	phylogeny	28:36	phylogeny	28:36	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	0	59	theme	PME	69:71	arg1	analysis					53:60	expression analysis	42:60	expression analysis	42:60	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	0	59	theme	PME	69:71	arg1	identification					12:25	Genome-wide identification	0:25	Genome-wide identification	0:25	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	4	60	theme	same	570:573	arg1	subgroup					575:582	the same subgroup	566:582	the same subgroup	566:582	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	2	61	theme	pectin	340:345	arg1	PMEIs					374:378	PMEIs	374:378	PMEIs	374:378	The PMEs are spatially regulated by pectin methylesterase inhibitors (PMEIs).					
31882640	2	61	theme	pectin	340:345	arg1	inhibitors					362:371	pectin methylesterase inhibitors	340:371	pectin methylesterase inhibitors (PMEIs)	340:379	The PMEs are spatially regulated by pectin methylesterase inhibitors (PMEIs).					
31882640	7	62	theme	ZmPME/PMEI	1278:1287	arg1	genes					1289:1293	these ZmPME/PMEI genes	1272:1293	these ZmPME/PMEI genes	1272:1293	In addition, these ZmPME/PMEI genes were highly expressed in the fertile anthers, while lowly or no expressed in sterile anthers.					
31882640	6	63	from	data	1079:1082	arg1	tissues					1035:1041	other tissues	1029:1041	other tissues from the NimbleGen maize microarray data	1029:1082	There are 35 ZmPME/PMEI genes expressed higher in anthers than in other tissues from the NimbleGen maize microarray data, and the semiq-RT-PCR assay revealed most of these ZmPME/PMEIs specially expressed in anthers and pollens, indicating they possibly had role in anther and pollen development.					
31882640	0	64	from	phylogeny	28:36	arg1	maize					99:103	maize	99:103	maize	99:103	Genome-wide identification, phylogeny and expression analysis of the PME and PMEI gene families in maize.					
31882640	4	65	dep	had	594:596	arg1	indicates					659:667	indicates	659:667	indicates functional similarity between the closely related ZmPME/PMEI genes	659:734	Gene structure and motif analysis revealed that members in the same paralogous pairs or in the same subgroup generally had common motif compositions and gene structure patterns, which indicates functional similarity between the closely related ZmPME/PMEI genes.					
31882640	1	66	from	walls	144:148	arg1	plants					153:158	plants	153:158	plants	153:158	Pectins, the major components of cell walls in plants, are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs).					
31882640	8	67	theme	pollen	1475:1480	arg1	development					1449:1459	the development	1445:1459	the development of anther and pollen	1445:1480	This further indicated these genes might be involved in the development of anther and pollen.					
29703974	6	0	theme	individual	1118:1127	arg1	composition					1139:1149	the individual cell wall composition	1114:1149	the individual cell wall composition of Gram-negative and Gram-positive organisms	1114:1194	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	6	1	from	involvement	1218:1228	arg1	virulence					1257:1265	virulence	1257:1265	virulence	1257:1265	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	1	2	theme	key	165:167	arg1	players					169:175	key players	165:175	key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes	165:317	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
29703974	2	3	from	prevalent	343:351	arg1	genomes					371:377	many bacterial genomes	356:377	many bacterial genomes	356:377	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	2	4	theme	bacterial	361:369	arg1	genomes					371:377	many bacterial genomes	356:377	many bacterial genomes	356:377	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	0	5	theme	wall	97:100	arg1	synthesis					102:110	cell wall synthesis	92:110	cell wall synthesis	92:110	The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system drains precursors for cell wall synthesis.					
29703974	2	6	from	synthesis	457:465	arg1	bacteria					484:491	Gram-positive bacteria	470:491	Gram-positive bacteria	470:491	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	3	7	theme	Neisseria	596:604	arg1	gonorrhoeae					606:616	the Gram-negative pathogen Neisseria gonorrhoeae	569:616	the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1	569:637	Here, we characterize the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1.					
29703974	0	8	theme	cell	92:95	arg1	synthesis					102:110	cell wall synthesis	92:110	cell wall synthesis	92:110	The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system drains precursors for cell wall synthesis.					
29703974	3	9	theme	the Gram-negative	569:585	arg1	gonorrhoeae					606:616	the Gram-negative pathogen Neisseria gonorrhoeae	569:616	the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1	569:637	Here, we characterize the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1.					
29703974	3	10	theme	epsilon/zeta	520:531	arg1	homologue					554:562	the epsilon/zeta toxin-antitoxin (TA) homologue	516:562	the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1	516:637	Here, we characterize the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1.					
29703974	4	11	theme	substrate	773:781	arg1	specificity					783:793	broader substrate specificity	765:793	broader substrate specificity	765:793	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	2	12	theme	many	356:359	arg1	genomes					371:377	many bacterial genomes	356:377	many bacterial genomes	356:377	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	6	13	theme	distinct	1209:1216	arg1	involvement					1218:1228	the distinct involvement	1205:1228	adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence	1100:1265	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	5	14	theme	phosphorylation	929:943	arg1	site					945:948	the phosphorylation site	925:948	the phosphorylation site	925:948	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	5	14	theme	phosphorylation	929:943	arg1	different					953:961	different	953:961	different	953:961	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	4	15	dep	peptidoglycan	867:879	arg1	synthesis					904:912	synthesis	904:912	synthesis	904:912	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	6	16	theme	Gram-negative	1154:1166	arg1	organisms					1186:1194	Gram-negative and Gram-positive organisms	1154:1194	Gram-negative and Gram-positive organisms	1154:1194	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	2	17	theme	Gram-positive	470:482	arg1	bacteria					484:491	Gram-positive bacteria	470:491	Gram-positive bacteria	470:491	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	5	18	with	interference	1024:1035	arg1	synthesis					1052:1060	cell wall synthesis	1042:1060	cell wall synthesis	1042:1060	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	2	19	from	genomes	371:377	arg1	prevalent					343:351	prevalent	343:351	prevalent	343:351	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	1	20	theme	bacterial	188:196	arg1	physiology					198:207	bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes	188:317	bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes	188:317	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
29703974	0	21	theme	ng_ζ1	4:8	arg1	toxin					10:14	The ng_ζ1 toxin	0:14	The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system	0:68	The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system drains precursors for cell wall synthesis.					
29703974	5	22	theme	wall	1047:1050	arg1	synthesis					1052:1060	cell wall synthesis	1042:1060	cell wall synthesis	1042:1060	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	3	23	dep	ng_ζ1	633:637	arg1	ng_ɛ1					625:629	ng_ɛ1	625:629	ng_ɛ1	625:629	Here, we characterize the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1.					
29703974	1	24	theme	vital	294:298	arg1	processes					309:317	vital cellular processes	294:317	vital cellular processes	294:317	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
29703974	6	25	from	adaptation	1100:1109	arg1	virulence					1257:1265	virulence	1257:1265	virulence	1257:1265	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	5	26	theme	cell	1042:1045	arg1	synthesis					1052:1060	cell wall synthesis	1042:1060	cell wall synthesis	1042:1060	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	5	27	theme	streptococcal	972:984	arg1	toxins					991:996	the streptococcal zeta toxins	968:996	the streptococcal zeta toxins	968:996	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	2	28	theme	peptidoglycan	443:455	arg1	synthesis					457:465	peptidoglycan synthesis	443:465	peptidoglycan synthesis in Gram-positive bacteria	443:491	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	1	29	theme	cellular	300:307	arg1	processes					309:317	vital cellular processes	294:317	vital cellular processes	294:317	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
29703974	5	30	from	toxins	991:996	arg1	site					945:948	the phosphorylation site	925:948	the phosphorylation site	925:948	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	5	30	from	toxins	991:996	arg1	different					953:961	different	953:961	different	953:961	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	3	31	theme	pathogen	587:594	arg1	gonorrhoeae					606:616	the Gram-negative pathogen Neisseria gonorrhoeae	569:616	the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1	569:637	Here, we characterize the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1.					
29703974	4	32	theme	epsilon/zeta	685:696	arg1	systems					701:707	previously studied streptococcal epsilon/zeta TA systems	652:707	previously studied streptococcal epsilon/zeta TA systems	652:707	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	6	33	theme	cell	1233:1236	arg1	components					1243:1252	cell wall components	1233:1252	cell wall components	1233:1252	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	2	34	theme	Zeta	320:323	arg1	toxins					325:330	Zeta toxins	320:330	Zeta toxins	320:330	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	4	35	theme	streptococcal	671:683	arg1	systems					701:707	previously studied streptococcal epsilon/zeta TA systems	652:707	previously studied streptococcal epsilon/zeta TA systems	652:707	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	0	36	theme	gonococcal	23:32	arg1	system					63:68	the gonococcal epsilon/zeta toxin/antitoxin system	19:68	the gonococcal epsilon/zeta toxin/antitoxin system	19:68	The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system drains precursors for cell wall synthesis.					
29703974	6	37	theme	components	1243:1252	arg1	adaptation					1100:1109	adaptation	1100:1109	adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence	1100:1265	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	6	37	theme	components	1243:1252	arg1	involvement					1218:1228	the distinct involvement	1205:1228	adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence	1100:1265	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	1	38	theme	toxins	226:231	arg1	activation					212:221	activation	212:221	activation of toxins	212:231	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
29703974	6	39	theme	Gram-positive	1172:1184	arg1	organisms					1186:1194	Gram-negative and Gram-positive organisms	1154:1194	Gram-negative and Gram-positive organisms	1154:1194	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	2	40	theme	wall	414:417	arg1	formation					419:427	cell wall formation	409:427	cell wall formation	409:427	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	6	41	theme	organisms	1186:1194	arg1	composition					1139:1149	the individual cell wall composition	1114:1149	the individual cell wall composition of Gram-negative and Gram-positive organisms	1114:1194	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	4	42	theme	studied	663:669	arg1	systems					701:707	previously studied streptococcal epsilon/zeta TA systems	652:707	previously studied streptococcal epsilon/zeta TA systems	652:707	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	1	43	with	interference	276:287	arg1	processes					309:317	vital cellular processes	294:317	vital cellular processes	294:317	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
29703974	2	44	theme	cell	409:412	arg1	formation					419:427	cell wall formation	409:427	cell wall formation	409:427	Zeta toxins, which are prevalent in many bacterial genomes, were shown to interfere with cell wall formation by perturbing peptidoglycan synthesis in Gram-positive bacteria.					
29703974	0	45	theme	toxin/antitoxin	47:61	arg1	system					63:68	the gonococcal epsilon/zeta toxin/antitoxin system	19:68	the gonococcal epsilon/zeta toxin/antitoxin system	19:68	The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system drains precursors for cell wall synthesis.					
29703974	6	46	theme	wall	1238:1241	arg1	components					1243:1252	cell wall components	1233:1252	cell wall components	1233:1252	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	1	47	theme	Bacterial	113:121	arg1	complexes					139:147	Bacterial toxin-antitoxin complexes	113:147	Bacterial toxin-antitoxin complexes	113:147	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
29703974	0	48	theme	epsilon/zeta	34:45	arg1	system					63:68	the gonococcal epsilon/zeta toxin/antitoxin system	19:68	the gonococcal epsilon/zeta toxin/antitoxin system	19:68	The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system drains precursors for cell wall synthesis.					
29703974	4	49	contain	has	716:718	arg1	ng_ɛ1					710:714	ng_ɛ1	710:714	ng_ɛ1	710:714	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	4	49	contain	has	716:718	arg2	fold					741:744	an epsilon-unrelated fold	720:744	an epsilon-unrelated fold	720:744	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	3	50	theme	toxin-antitoxin	533:547	arg1	homologue					554:562	the epsilon/zeta toxin-antitoxin (TA) homologue	516:562	the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1	516:637	Here, we characterize the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1.					
29703974	1	51	theme	toxin-antitoxin	123:137	arg1	complexes					139:147	Bacterial toxin-antitoxin complexes	113:147	Bacterial toxin-antitoxin complexes	113:147	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
29703974	4	52	theme	broader	765:771	arg1	specificity					783:793	broader substrate specificity	765:793	broader substrate specificity	765:793	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	4	53	theme	peptidoglycan	867:879	arg1	sugars					837:842	multiple UDP-activated sugars	814:842	multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis	814:912	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	4	53	theme	peptidoglycan	867:879	arg1	precursors					853:862	precursors	853:862	precursors of peptidoglycan and lipopolysaccharide synthesis	853:912	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	5	54	theme	different	1014:1022	arg1	interference					1024:1035	a different interference	1012:1035	a different interference with cell wall synthesis	1012:1060	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	4	55	theme	UDP-activated	823:835	arg1	sugars					837:842	multiple UDP-activated sugars	814:842	multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis	814:912	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	4	55	theme	UDP-activated	823:835	arg1	precursors					853:862	precursors	853:862	precursors of peptidoglycan and lipopolysaccharide synthesis	853:912	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	6	56	theme	wall	1134:1137	arg1	composition					1139:1149	the individual cell wall composition	1114:1149	the individual cell wall composition of Gram-negative and Gram-positive organisms	1114:1194	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	4	57	theme	multiple	814:821	arg1	sugars					837:842	multiple UDP-activated sugars	814:842	multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis	814:912	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	4	57	theme	multiple	814:821	arg1	precursors					853:862	precursors	853:862	precursors of peptidoglycan and lipopolysaccharide synthesis	853:912	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	4	58	theme	epsilon-unrelated	723:739	arg1	fold					741:744	an epsilon-unrelated fold	720:744	an epsilon-unrelated fold	720:744	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	3	59	from	gonorrhoeae	606:616	arg1	homologue					554:562	the epsilon/zeta toxin-antitoxin (TA) homologue	516:562	the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1	516:637	Here, we characterize the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1.					
29703974	6	60	theme	cell	1129:1132	arg1	composition					1139:1149	the individual cell wall composition	1114:1149	the individual cell wall composition of Gram-negative and Gram-positive organisms	1114:1194	This difference most likely reflects adaptation to the individual cell wall composition of Gram-negative and Gram-positive organisms but also the distinct involvement of cell wall components in virulence.					
29703974	4	61	theme	TA	698:699	arg1	systems					701:707	previously studied streptococcal epsilon/zeta TA systems	652:707	previously studied streptococcal epsilon/zeta TA systems	652:707	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	4	62	theme	lipopolysaccharide	885:902	arg1	sugars					837:842	multiple UDP-activated sugars	814:842	multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis	814:912	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	4	62	theme	lipopolysaccharide	885:902	arg1	precursors					853:862	precursors	853:862	precursors of peptidoglycan and lipopolysaccharide synthesis	853:912	Contrary to previously studied streptococcal epsilon/zeta TA systems, ng_ɛ1 has an epsilon-unrelated fold and ng_ζ1 displays broader substrate specificity and phosphorylates multiple UDP-activated sugars that are precursors of peptidoglycan and lipopolysaccharide synthesis.					
29703974	5	63	theme	zeta	986:989	arg1	toxins					991:996	the streptococcal zeta toxins	968:996	the streptococcal zeta toxins	968:996	Moreover, the phosphorylation site is different from the streptococcal zeta toxins, resulting in a different interference with cell wall synthesis.					
29703974	1	64	theme	programmed	251:260	arg1	death					267:271	programmed cell death	251:271	programmed cell death	251:271	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
29703974	0	65	theme	system	63:68	arg1	toxin					10:14	The ng_ζ1 toxin	0:14	The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system	0:68	The ng_ζ1 toxin of the gonococcal epsilon/zeta toxin/antitoxin system drains precursors for cell wall synthesis.					
29703974	3	66	theme	TA	550:551	arg1	homologue					554:562	the epsilon/zeta toxin-antitoxin (TA) homologue	516:562	the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1	516:637	Here, we characterize the epsilon/zeta toxin-antitoxin (TA) homologue from the Gram-negative pathogen Neisseria gonorrhoeae termed ng_ɛ1 / ng_ζ1.					
29703974	1	67	theme	cell	262:265	arg1	death					267:271	programmed cell death	251:271	programmed cell death	251:271	Bacterial toxin-antitoxin complexes are emerging as key players modulating bacterial physiology as activation of toxins induces stasis or programmed cell death by interference with vital cellular processes.					
30904531	0	0	theme	electrospun	84:94	arg1	membrane					108:115	chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane	52:115	chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane	52:115	Degradation of methyl orange and congo red by using chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane.					
30904531	9	1	theme	isotherm	977:984	arg1	models					986:991	both Langmuir and Freundlich isotherm models	948:991	models	986:991	On the other hand, adsorption characteristic of methyl orange fitted well with both Langmuir and Freundlich isotherm models.					
30904531	11	2	theme	high	1130:1133	arg1	dose					1135:1138	a high dose	1128:1138	a high dose of adsorbent	1128:1151	However, a high dose of adsorbent was required for congo red.					
30904531	8	3	theme	Langmuir	844:851	arg1	model					862:866	the Langmuir isotherm model	840:866	the Langmuir isotherm model	840:866	The adsorption mechanism of congo red obeyed the Langmuir isotherm model.					
30904531	0	4	theme	alcohol/TiO2	71:82	arg1	membrane					108:115	chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane	52:115	chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane	52:115	Degradation of methyl orange and congo red by using chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane.					
30904531	2	5	theme	free	319:322	arg1	nanofiber					330:338	bead free finer nanofiber	314:338	bead free finer nanofiber	314:338	Field-emission scanning electron microscopic images showed bead free finer nanofiber.					
30904531	8	6	theme	red	829:831	arg1	mechanism					810:818	The adsorption mechanism	795:818	The adsorption mechanism of congo red	795:831	The adsorption mechanism of congo red obeyed the Langmuir isotherm model.					
30904531	10	7	theme	membrane	1043:1050	arg1	capacity					1017:1024	The maximum adsorption capacity	994:1024	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange	994:1082	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange is 131 and 314 mg/g, respectively.					
30904531	10	7	theme	membrane	1043:1050	arg1	131					1087:1089	131	1087:1089	131	1087:1089	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange is 131 and 314 mg/g, respectively.					
30904531	0	8	theme	nanofibrous	96:106	arg1	membrane					108:115	chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane	52:115	chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane	52:115	Degradation of methyl orange and congo red by using chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane.					
30904531	9	9	theme	other	876:880	arg1	hand					882:885	the other hand	872:885	the other hand	872:885	On the other hand, adsorption characteristic of methyl orange fitted well with both Langmuir and Freundlich isotherm models.					
30904531	2	10	theme	bead	314:317	arg1	nanofiber					330:338	bead free finer nanofiber	314:338	bead free finer nanofiber	314:338	Field-emission scanning electron microscopic images showed bead free finer nanofiber.					
30904531	8	11	theme	isotherm	853:860	arg1	model					862:866	the Langmuir isotherm model	840:866	the Langmuir isotherm model	840:866	The adsorption mechanism of congo red obeyed the Langmuir isotherm model.					
30904531	3	12	dep	Fourier	341:347	arg1	transform					349:357	transform	349:357	transform infrared spectra	349:374	Fourier transform infrared spectra proved the formation of strong bond among chitosan, polyvinyl alcohol and TiO2.					
30904531	4	13	theme	composite	522:530	arg1	nanofiber					532:540	the composite nanofiber	518:540	the composite nanofiber	518:540	X-ray powder diffraction showed that TiO2 became amorphous in the composite nanofiber.					
30904531	6	14	theme	nanofibrous	672:682	arg1	stable					697:702	stable	697:702	stable	697:702	The chitosan/PVA/TiO2 nanofibrous membrane was stable at basic medium.					
30904531	6	14	theme	nanofibrous	672:682	arg1	membrane					684:691	The chitosan/PVA/TiO2 nanofibrous membrane	650:691	The chitosan/PVA/TiO2 nanofibrous membrane	650:691	The chitosan/PVA/TiO2 nanofibrous membrane was stable at basic medium.					
30904531	3	15	theme	infrared	359:366	arg1	spectra					368:374	infrared spectra	359:374	infrared spectra	359:374	Fourier transform infrared spectra proved the formation of strong bond among chitosan, polyvinyl alcohol and TiO2.					
30904531	9	16	theme	adsorption	888:897	arg1	characteristic					899:912	adsorption characteristic	888:912	adsorption characteristic of methyl orange	888:929	On the other hand, adsorption characteristic of methyl orange fitted well with both Langmuir and Freundlich isotherm models.					
30904531	10	17	theme	adsorption	1006:1015	arg1	capacity					1017:1024	The maximum adsorption capacity	994:1024	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange	994:1082	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange is 131 and 314 mg/g, respectively.					
30904531	10	17	theme	adsorption	1006:1015	arg1	131					1087:1089	131	1087:1089	131	1087:1089	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange is 131 and 314 mg/g, respectively.					
30904531	6	18	theme	chitosan/PVA/TiO2	654:670	arg1	stable					697:702	stable	697:702	stable	697:702	The chitosan/PVA/TiO2 nanofibrous membrane was stable at basic medium.					
30904531	6	18	theme	chitosan/PVA/TiO2	654:670	arg1	membrane					684:691	The chitosan/PVA/TiO2 nanofibrous membrane	650:691	The chitosan/PVA/TiO2 nanofibrous membrane	650:691	The chitosan/PVA/TiO2 nanofibrous membrane was stable at basic medium.					
30904531	5	19	theme	addition	635:642	arg1	TiO2					644:647	addition TiO2	635:647	addition TiO2	635:647	Toughness and thermal stability of the chitosan/PVA nanofibrous membrane was increased with addition TiO2.					
30904531	7	20	theme	acidic	737:742	arg1	medium					754:759	acidic and water medium	737:759	medium	754:759	But degraded in acidic and water medium after 93 and 162 h, respectively.					
30904531	3	21	theme	bond	407:410	arg1	formation					387:395	the formation	383:395	the formation of strong bond among chitosan, polyvinyl alcohol and TiO2	383:453	Fourier transform infrared spectra proved the formation of strong bond among chitosan, polyvinyl alcohol and TiO2.					
30904531	10	22	theme	resulting	1033:1041	arg1	membrane					1043:1050	the resulting membrane	1029:1050	the resulting membrane	1029:1050	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange is 131 and 314 mg/g, respectively.					
30904531	1	23	theme	pump	215:218	arg1	rate					220:223	a pump rate	213:223	a pump rate of 1.5 mL/h and voltage 6 kV	213:252	In this study, chitosan/polyvinyl alcohol/TiO2 nanofiber was fabricated via electrospinning at a pump rate of 1.5 mL/h and voltage 6 kV.					
30904531	0	24	theme	methyl	15:20	arg1	orange					22:27	methyl orange	15:27	methyl orange	15:27	Degradation of methyl orange and congo red by using chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane.					
30904531	9	25	theme	methyl	917:922	arg1	orange					924:929	methyl orange	917:929	methyl orange	917:929	On the other hand, adsorption characteristic of methyl orange fitted well with both Langmuir and Freundlich isotherm models.					
30904531	2	26	theme	finer	324:328	arg1	nanofiber					330:338	bead free finer nanofiber	314:338	bead free finer nanofiber	314:338	Field-emission scanning electron microscopic images showed bead free finer nanofiber.					
30904531	9	27	theme	orange	924:929	arg1	characteristic					899:912	adsorption characteristic	888:912	adsorption characteristic of methyl orange	888:929	On the other hand, adsorption characteristic of methyl orange fitted well with both Langmuir and Freundlich isotherm models.					
30904531	6	28	from	medium	713:718	arg1	stable					697:702	stable	697:702	stable	697:702	The chitosan/PVA/TiO2 nanofibrous membrane was stable at basic medium.					
30904531	6	28	from	medium	713:718	arg1	membrane					684:691	The chitosan/PVA/TiO2 nanofibrous membrane	650:691	The chitosan/PVA/TiO2 nanofibrous membrane	650:691	The chitosan/PVA/TiO2 nanofibrous membrane was stable at basic medium.					
30904531	7	29	theme	water	748:752	arg1	medium					754:759	acidic and water medium	737:759	medium	754:759	But degraded in acidic and water medium after 93 and 162 h, respectively.					
30904531	0	30	theme	orange	22:27	arg1	Degradation					0:10	Degradation	0:10	Degradation of methyl orange and congo red by using chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane.	0:116	Degradation of methyl orange and congo red by using chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane.					
30904531	5	31	theme	chitosan/PVA	582:593	arg1	membrane					607:614	the chitosan/PVA nanofibrous membrane	578:614	the chitosan/PVA nanofibrous membrane	578:614	Toughness and thermal stability of the chitosan/PVA nanofibrous membrane was increased with addition TiO2.					
30904531	1	32	theme	1.5 mL/h	228:235	arg1	rate					220:223	a pump rate	213:223	a pump rate of 1.5 mL/h and voltage 6 kV	213:252	In this study, chitosan/polyvinyl alcohol/TiO2 nanofiber was fabricated via electrospinning at a pump rate of 1.5 mL/h and voltage 6 kV.					
30904531	3	33	theme	polyvinyl	428:436	arg1	alcohol					438:444	polyvinyl alcohol	428:444	polyvinyl alcohol	428:444	Fourier transform infrared spectra proved the formation of strong bond among chitosan, polyvinyl alcohol and TiO2.					
30904531	11	34	theme	adsorbent	1143:1151	arg1	dose					1135:1138	a high dose	1128:1138	a high dose of adsorbent	1128:1151	However, a high dose of adsorbent was required for congo red.					
30904531	5	35	theme	nanofibrous	595:605	arg1	membrane					607:614	the chitosan/PVA nanofibrous membrane	578:614	the chitosan/PVA nanofibrous membrane	578:614	Toughness and thermal stability of the chitosan/PVA nanofibrous membrane was increased with addition TiO2.					
30904531	0	36	theme	red	39:41	arg1	Degradation					0:10	Degradation	0:10	Degradation of methyl orange and congo red by using chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane.	0:116	Degradation of methyl orange and congo red by using chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane.					
30904531	4	37	theme	X-ray	456:460	arg1	diffraction					469:479	X-ray powder diffraction	456:479	X-ray powder diffraction	456:479	X-ray powder diffraction showed that TiO2 became amorphous in the composite nanofiber.					
30904531	5	38	theme	membrane	607:614	arg1	Toughness					543:551	Toughness	543:551	Toughness	543:551	Toughness and thermal stability of the chitosan/PVA nanofibrous membrane was increased with addition TiO2.					
30904531	5	38	theme	membrane	607:614	arg1	stability					565:573	thermal stability	557:573	thermal stability	557:573	Toughness and thermal stability of the chitosan/PVA nanofibrous membrane was increased with addition TiO2.					
30904531	10	39	theme	methyl	1070:1075	arg1	orange					1077:1082	methyl orange	1070:1082	methyl orange	1070:1082	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange is 131 and 314 mg/g, respectively.					
30904531	8	40	theme	adsorption	799:808	arg1	mechanism					810:818	The adsorption mechanism	795:818	The adsorption mechanism of congo red	795:831	The adsorption mechanism of congo red obeyed the Langmuir isotherm model.					
30904531	2	41	theme	microscopic	288:298	arg1	images					300:305	Field-emission scanning electron microscopic images	255:305	Field-emission scanning electron microscopic images	255:305	Field-emission scanning electron microscopic images showed bead free finer nanofiber.					
30904531	10	42	theme	maximum	998:1004	arg1	capacity					1017:1024	The maximum adsorption capacity	994:1024	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange	994:1082	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange is 131 and 314 mg/g, respectively.					
30904531	10	42	theme	maximum	998:1004	arg1	131					1087:1089	131	1087:1089	131	1087:1089	The maximum adsorption capacity of the resulting membrane for congo red and methyl orange is 131 and 314 mg/g, respectively.					
30904531	2	43	theme	electron	279:286	arg1	images					300:305	Field-emission scanning electron microscopic images	255:305	Field-emission scanning electron microscopic images	255:305	Field-emission scanning electron microscopic images showed bead free finer nanofiber.					
30904531	5	44	theme	thermal	557:563	arg1	stability					565:573	thermal stability	557:573	thermal stability	557:573	Toughness and thermal stability of the chitosan/PVA nanofibrous membrane was increased with addition TiO2.					
30904531	1	45	theme	chitosan/polyvinyl	133:150	arg1	nanofiber					165:173	chitosan/polyvinyl alcohol/TiO2 nanofiber	133:173	chitosan/polyvinyl alcohol/TiO2 nanofiber	133:173	In this study, chitosan/polyvinyl alcohol/TiO2 nanofiber was fabricated via electrospinning at a pump rate of 1.5 mL/h and voltage 6 kV.					
30904531	1	46	theme	voltage	241:247	arg1	6 kV					249:252	voltage 6 kV	241:252	voltage 6 kV	241:252	In this study, chitosan/polyvinyl alcohol/TiO2 nanofiber was fabricated via electrospinning at a pump rate of 1.5 mL/h and voltage 6 kV.					
30904531	0	47	theme	chitosan/polyvinyl	52:69	arg1	membrane					108:115	chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane	52:115	chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane	52:115	Degradation of methyl orange and congo red by using chitosan/polyvinyl alcohol/TiO2 electrospun nanofibrous membrane.					
30904531	4	48	theme	powder	462:467	arg1	diffraction					469:479	X-ray powder diffraction	456:479	X-ray powder diffraction	456:479	X-ray powder diffraction showed that TiO2 became amorphous in the composite nanofiber.					
30904531	2	49	theme	scanning	270:277	arg1	images					300:305	Field-emission scanning electron microscopic images	255:305	Field-emission scanning electron microscopic images	255:305	Field-emission scanning electron microscopic images showed bead free finer nanofiber.					
30904531	1	50	theme	alcohol/TiO2	152:163	arg1	nanofiber					165:173	chitosan/polyvinyl alcohol/TiO2 nanofiber	133:173	chitosan/polyvinyl alcohol/TiO2 nanofiber	133:173	In this study, chitosan/polyvinyl alcohol/TiO2 nanofiber was fabricated via electrospinning at a pump rate of 1.5 mL/h and voltage 6 kV.					
30904531	6	51	theme	basic	707:711	arg1	medium					713:718	basic medium	707:718	basic medium	707:718	The chitosan/PVA/TiO2 nanofibrous membrane was stable at basic medium.					
30904531	1	52	theme	6 kV	249:252	arg1	rate					220:223	a pump rate	213:223	a pump rate of 1.5 mL/h and voltage 6 kV	213:252	In this study, chitosan/polyvinyl alcohol/TiO2 nanofiber was fabricated via electrospinning at a pump rate of 1.5 mL/h and voltage 6 kV.					
30904531	9	53	theme	Freundlich	966:975	arg1	models					986:991	both Langmuir and Freundlich isotherm models	948:991	models	986:991	On the other hand, adsorption characteristic of methyl orange fitted well with both Langmuir and Freundlich isotherm models.					
30904531	2	54	theme	Field-emission	255:268	arg1	images					300:305	Field-emission scanning electron microscopic images	255:305	Field-emission scanning electron microscopic images	255:305	Field-emission scanning electron microscopic images showed bead free finer nanofiber.					
30904531	3	55	theme	strong	400:405	arg1	bond					407:410	strong bond	400:410	strong bond	400:410	Fourier transform infrared spectra proved the formation of strong bond among chitosan, polyvinyl alcohol and TiO2.					
30115879	3	0	from	sequencing	645:654	arg1	platform					705:712	the MiSeq platform	695:712	the MiSeq platform	695:712	In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3⁻V4 region and the groEL gene on the MiSeq platform, respectively.					
30115879	3	1	from	FOS	519:521	arg1	doses					536:540	different doses	526:540	different doses	526:540	In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3⁻V4 region and the groEL gene on the MiSeq platform, respectively.					
30115879	6	2	theme	Bifidobacterium	1022:1036	arg1	species					1011:1017	14 species	1008:1017	14 species of Bifidobacterium	1008:1036	Through the groEL sequencing, 14 species of Bifidobacterium were identified, among which B.pseudolongum was most abundant.					
30115879	3	3	theme	V3⁻V4	660:664	arg1	region					666:671	the V3⁻V4 region	656:671	the V3⁻V4 region	656:671	In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3⁻V4 region and the groEL gene on the MiSeq platform, respectively.					
30115879	7	4	theme	FOS	1107:1109	arg1	treatment					1111:1119	FOS treatment	1107:1119	FOS treatment	1107:1119	After FOS treatment, B.pseudolongum became almost the sole bifidobacterial species (>95%).					
30115879	9	5	theme	B.pseudolongum	1399:1412	arg1	growth					1389:1394	the growth	1385:1394	the growth of B.pseudolongum in mice	1385:1420	Therefore, we inferred that FOS significantly stimulated the growth of B.pseudolongum in mice.					
30115879	0	6	theme	Mice	81:84	arg1	Composition					66:76	the Composition	62:76	the Composition of Mice	62:84	Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition.					
30115879	0	7	from	Effects	0:6	arg1	Composition					66:76	the Composition	62:76	the Composition of Mice	62:84	Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition.					
30115879	10	8	theme	Further	1423:1429	arg1	investigations					1431:1444	Further investigations	1423:1444	Further investigations	1423:1444	Further investigations are needed to reveal the mechanism of selectiveness between FOS and B.pseudolongum, which would aid our understanding of the basic principles between dietary carbohydrates and host health.					
30115879	5	9	theme	Coprococcus	931:941	arg1	abundances					905:914	the relative abundances	892:914	the relative abundances of Blautia and Coprococcus	892:941	At the genus level, the relative abundances of Blautia and Coprococcus were also significantly increased.					
30115879	3	10	theme	different	526:534	arg1	doses					536:540	different doses	526:540	different doses	526:540	In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3⁻V4 region and the groEL gene on the MiSeq platform, respectively.					
30115879	5	11	theme	genus	879:883	arg1	level					885:889	the genus level	875:889	the genus level	875:889	At the genus level, the relative abundances of Blautia and Coprococcus were also significantly increased.					
30115879	4	12	theme	high-dose	736:744	arg1	group					746:750	the high-dose group	732:750	the high-dose group	732:750	In the high-dose group, the relative abundance of Actinobacteria was significantly increased, which was mainly contributed by Bifidobacterium.					
30115879	3	13	theme	groEL	681:685	arg1	gene					687:690	the groEL gene	677:690	the groEL gene	677:690	In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3⁻V4 region and the groEL gene on the MiSeq platform, respectively.					
30115879	4	14	theme	relative	757:764	arg1	abundance					766:774	the relative abundance	753:774	the relative abundance of Actinobacteria	753:792	In the high-dose group, the relative abundance of Actinobacteria was significantly increased, which was mainly contributed by Bifidobacterium.					
30115879	10	15	theme	dietary	1596:1602	arg1	carbohydrates					1604:1616	dietary carbohydrates	1596:1616	dietary carbohydrates	1596:1616	Further investigations are needed to reveal the mechanism of selectiveness between FOS and B.pseudolongum, which would aid our understanding of the basic principles between dietary carbohydrates and host health.					
30115879	2	16	theme	specific	446:453	arg1	species					455:461	the specific species	442:461	the specific species that were stimulated	442:482	Previous studies focused on the growth stimulation of Bifidobacterium, but they did not further investigate the bifidobacterial composition and the specific species that were stimulated.					
30115879	5	17	theme	relative	896:903	arg1	abundances					905:914	the relative abundances	892:914	the relative abundances of Blautia and Coprococcus	892:941	At the genus level, the relative abundances of Blautia and Coprococcus were also significantly increased.					
30115879	7	18	theme	sole	1155:1158	arg1	species					1176:1182	almost the sole bifidobacterial species	1144:1182	almost the sole bifidobacterial species (>95%)	1144:1189	After FOS treatment, B.pseudolongum became almost the sole bifidobacterial species (>95%).					
30115879	7	18	theme	sole	1155:1158	arg1	%					1188:1188	>95%	1185:1188	>95%	1185:1188	After FOS treatment, B.pseudolongum became almost the sole bifidobacterial species (>95%).					
30115879	7	19	theme	bifidobacterial	1160:1174	arg1	species					1176:1182	almost the sole bifidobacterial species	1144:1182	almost the sole bifidobacterial species (>95%)	1144:1189	After FOS treatment, B.pseudolongum became almost the sole bifidobacterial species (>95%).					
30115879	7	19	theme	bifidobacterial	1160:1174	arg1	%					1188:1188	>95%	1185:1188	>95%	1185:1188	After FOS treatment, B.pseudolongum became almost the sole bifidobacterial species (>95%).					
30115879	2	20	theme	Previous	298:305	arg1	studies					307:313	Previous studies	298:313	Previous studies	298:313	Previous studies focused on the growth stimulation of Bifidobacterium, but they did not further investigate the bifidobacterial composition and the specific species that were stimulated.					
30115879	0	21	theme	Doses	21:25	arg1	Effects					0:6	Effects	0:6	Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice	0:84	Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition.					
30115879	1	22	theme	bifidobacteria	271:284	arg1	growth					261:266	the growth	257:266	the growth of bifidobacteria in the gut	257:295	Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut.					
30115879	2	23	theme	bifidobacterial	410:424	arg1	composition					426:436	the bifidobacterial composition	406:436	the bifidobacterial composition	406:436	Previous studies focused on the growth stimulation of Bifidobacterium, but they did not further investigate the bifidobacterial composition and the specific species that were stimulated.					
30115879	0	24	theme	Different	11:19	arg1	Doses					21:25	Different Doses	11:25	Different Doses of Fructooligosaccharides (FOS)	11:57	Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition.					
30115879	3	25	theme	MiSeq	699:703	arg1	platform					705:712	the MiSeq platform	695:712	the MiSeq platform	695:712	In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3⁻V4 region and the groEL gene on the MiSeq platform, respectively.					
30115879	1	26	from	growth	261:266	arg1	gut					293:295	the gut	289:295	the gut	289:295	Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut.					
30115879	5	27	theme	Blautia	919:925	arg1	abundances					905:914	the relative abundances	892:914	the relative abundances of Blautia and Coprococcus	892:941	At the genus level, the relative abundances of Blautia and Coprococcus were also significantly increased.					
30115879	0	28	theme	Fructooligosaccharides	30:51	arg1	Doses					21:25	Different Doses	11:25	Different Doses of Fructooligosaccharides (FOS)	11:57	Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition.					
30115879	0	29	theme	Bifidobacterium	119:133	arg1	Composition					135:145	the Bifidobacterium Composition	115:145	Especially the Bifidobacterium Composition	104:145	Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition.					
30115879	0	29	theme	Bifidobacterium	119:133	arg1	Microbiota					92:101	Microbiota	92:101	Microbiota	92:101	Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition.					
30115879	10	30	theme	principles	1577:1586	arg1	understanding					1550:1562	our understanding	1546:1562	our understanding of the basic principles between dietary carbohydrates and host health	1546:1632	Further investigations are needed to reveal the mechanism of selectiveness between FOS and B.pseudolongum, which would aid our understanding of the basic principles between dietary carbohydrates and host health.					
30115879	8	31	theme	liquid	1298:1303	arg1	chromatography					1305:1318	high performance liquid chromatography	1281:1318	high performance liquid chromatography (HPLC)	1281:1325	B.pseudolongum strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC).					
30115879	8	31	theme	liquid	1298:1303	arg1	HPLC					1321:1324	HPLC	1321:1324	HPLC	1321:1324	B.pseudolongum strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC).					
30115879	1	32	theme	prebiotic	203:211	arg1	class					194:198	a well-known class	181:198	a well-known class of prebiotic	181:211	Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut.					
30115879	1	32	theme	prebiotic	203:211	arg1	Fructooligosaccharides					148:169	Fructooligosaccharides	148:169	Fructooligosaccharides (FOS)	148:175	Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut.					
30115879	10	33	theme	host	1622:1625	arg1	health					1627:1632	host health	1622:1632	host health	1622:1632	Further investigations are needed to reveal the mechanism of selectiveness between FOS and B.pseudolongum, which would aid our understanding of the basic principles between dietary carbohydrates and host health.					
30115879	10	34	theme	basic	1571:1575	arg1	principles					1577:1586	the basic principles	1567:1586	the basic principles between dietary carbohydrates and host health	1567:1632	Further investigations are needed to reveal the mechanism of selectiveness between FOS and B.pseudolongum, which would aid our understanding of the basic principles between dietary carbohydrates and host health.					
30115879	2	35	theme	growth	330:335	arg1	stimulation					337:347	the growth stimulation	326:347	the growth stimulation of Bifidobacterium	326:366	Previous studies focused on the growth stimulation of Bifidobacterium, but they did not further investigate the bifidobacterial composition and the specific species that were stimulated.					
30115879	8	36	theme	B.pseudolongum	1192:1205	arg1	strains					1207:1213	B.pseudolongum strains	1192:1213	B.pseudolongum strains	1192:1213	B.pseudolongum strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC).					
30115879	9	37	from	growth	1389:1394	arg1	mice					1417:1420	mice	1417:1420	mice	1417:1420	Therefore, we inferred that FOS significantly stimulated the growth of B.pseudolongum in mice.					
30115879	4	38	theme	Actinobacteria	779:792	arg1	abundance					766:774	the relative abundance	753:774	the relative abundance of Actinobacteria	753:792	In the high-dose group, the relative abundance of Actinobacteria was significantly increased, which was mainly contributed by Bifidobacterium.					
30115879	8	39	theme	performance	1286:1296	arg1	chromatography					1305:1318	high performance liquid chromatography	1281:1318	high performance liquid chromatography (HPLC)	1281:1325	B.pseudolongum strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC).					
30115879	8	39	theme	performance	1286:1296	arg1	HPLC					1321:1324	HPLC	1321:1324	HPLC	1321:1324	B.pseudolongum strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC).					
30115879	8	40	theme	high	1281:1284	arg1	chromatography					1305:1318	high performance liquid chromatography	1281:1318	high performance liquid chromatography (HPLC)	1281:1325	B.pseudolongum strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC).					
30115879	8	40	theme	high	1281:1284	arg1	HPLC					1321:1324	HPLC	1321:1324	HPLC	1321:1324	B.pseudolongum strains were isolated and demonstrated their ability to metabolize FOS by high performance liquid chromatography (HPLC).					
30115879	10	41	theme	selectiveness	1484:1496	arg1	mechanism					1471:1479	the mechanism	1467:1479	the mechanism of selectiveness	1467:1496	Further investigations are needed to reveal the mechanism of selectiveness between FOS and B.pseudolongum, which would aid our understanding of the basic principles between dietary carbohydrates and host health.					
30115879	3	42	theme	fecal	580:584	arg1	microbiota					586:595	fecal microbiota	580:595	fecal microbiota	580:595	In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3⁻V4 region and the groEL gene on the MiSeq platform, respectively.					
30115879	1	43	theme	well-known	183:192	arg1	class					194:198	a well-known class	181:198	a well-known class of prebiotic	181:211	Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut.					
30115879	1	43	theme	well-known	183:192	arg1	Fructooligosaccharides					148:169	Fructooligosaccharides	148:169	Fructooligosaccharides (FOS)	148:175	Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut.					
30115879	6	44	theme	groEL	990:994	arg1	sequencing					996:1005	the groEL sequencing	986:1005	the groEL sequencing	986:1005	Through the groEL sequencing, 14 species of Bifidobacterium were identified, among which B.pseudolongum was most abundant.					
30115879	3	45	theme	microbiota	586:595	arg1	composition					565:575	the composition	561:575	the composition of fecal microbiota	561:595	In this study, mice were fed with FOS in different doses for four weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3⁻V4 region and the groEL gene on the MiSeq platform, respectively.					
30115879	2	46	theme	Bifidobacterium	352:366	arg1	stimulation					337:347	the growth stimulation	326:347	the growth stimulation of Bifidobacterium	326:366	Previous studies focused on the growth stimulation of Bifidobacterium, but they did not further investigate the bifidobacterial composition and the specific species that were stimulated.					
31923961	4	0	theme	freeze-dried	910:921	arg1	aerogels					943:950	other reported freeze-dried nanocellulose-based aerogels	895:950	other reported freeze-dried nanocellulose-based aerogels	895:950	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	6	1	theme	molecular	1175:1183	arg1	weight					1185:1190	low molecular weight	1171:1190	low molecular weight S-nitrosothiols (RSNOs)	1171:1214	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	4	2	theme	similar	884:890	arg1	450 kPa					875:881	450 kPa	875:881	450 kPa	875:881	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	1	3	theme	ratio	225:229	arg1	structures					231:240	polymer-coated copper-cystine high-aspect ratio structures	183:240	polymer-coated copper-cystine high-aspect ratio structures (CuHARS)	183:249	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	1	3	theme	ratio	225:229	arg1	CuHARS					243:248	CuHARS	243:248	CuHARS	243:248	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	4	4	theme	other	895:899	arg1	aerogels					943:950	other reported freeze-dried nanocellulose-based aerogels	895:950	other reported freeze-dried nanocellulose-based aerogels	895:950	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	7	5	theme	redox	1443:1447	arg1	species					1449:1455	redox species	1443:1455	redox species	1443:1455	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	6	6	from	effective	1137:1145	arg1	decomposition					1154:1166	the decomposition	1150:1166	the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO)	1150:1279	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	10	7	theme	further	2161:2167	arg1	studies					2180:2186	further biological studies	2161:2186	further biological studies	2161:2186	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	9	8	theme	anti-inflammatory	1968:1984	arg1	effects					2006:2012	anti-inflammatory and anti-thrombotic effects	1968:2012	anti-inflammatory and anti-thrombotic effects	1968:2012	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	1	9	theme	cellulose	255:263	arg1	fibers					265:270	cellulose fibers	255:270	cellulose fibers	255:270	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	9	10	theme	NO	1782:1783	arg1	amount					1762:1767	the amount	1758:1767	the amount of generated NO (around 360 nM)	1758:1799	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	9	10	theme	NO	1782:1783	arg1	NO					1782:1783	generated NO	1772:1783	generated NO (around 360 nM)	1772:1799	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	9	11	theme	anti-thrombotic	1990:2004	arg1	effects					2006:2012	anti-inflammatory and anti-thrombotic effects	1968:2012	anti-inflammatory and anti-thrombotic effects	1968:2012	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	10	12	theme	cytotoxic	2019:2027	arg1	significant					2107:2117	significant	2107:2117	significant	2107:2117	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	12	theme	cytotoxic	2019:2027	arg1	effect					2029:2034	The cytotoxic effect	2015:2034	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs)	2015:2096	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	12	theme	cytotoxic	2019:2027	arg1	least					2101:2105	least	2101:2105	least	2101:2105	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	2	13	theme	metal-organic	292:304	arg1	materials					306:314	the metal-organic materials	288:314	the metal-organic materials with polyallylamine hydrochloride (PAH)	288:354	The coating of the metal-organic materials with polyallylamine hydrochloride (PAH) allows their covalent linkage to TEMPO-oxidized cellulose by means of EDC/NHS.					
31923961	5	14	theme	cellulose/PAH-CuHARS	989:1008	arg1	promising					1025:1033	promising	1025:1033	promising	1025:1033	Based on these characteristics, the cellulose/PAH-CuHARS composites are promising for potential biomedical applications as implants or wound dressing materials.					
31923961	5	14	theme	cellulose/PAH-CuHARS	989:1008	arg1	composites					1010:1019	the cellulose/PAH-CuHARS composites	985:1019	the cellulose/PAH-CuHARS composites	985:1019	Based on these characteristics, the cellulose/PAH-CuHARS composites are promising for potential biomedical applications as implants or wound dressing materials.					
31923961	8	15	theme	resulting	1507:1515	arg1	biomaterials					1517:1528	The resulting biomaterials	1503:1528	The resulting biomaterials	1503:1528	The resulting biomaterials can offer the interesting properties associated with NO, like antimicrobial activity as preliminary tests showed here with Escherichia coli and Staphylococcus epidermidis.					
31923961	4	16	theme	interconnected	715:728	arg1	macropores					730:739	interconnected macropores	715:739	interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels	715:950	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	8	17	theme	preliminary	1618:1628	arg1	tests					1630:1634	preliminary tests	1618:1634	preliminary tests showed here with Escherichia coli and Staphylococcus epidermidis	1618:1699	The resulting biomaterials can offer the interesting properties associated with NO, like antimicrobial activity as preliminary tests showed here with Escherichia coli and Staphylococcus epidermidis.					
31923961	8	17	theme	preliminary	1618:1628	arg1	properties					1556:1565	the interesting properties	1540:1565	the interesting properties	1540:1565	The resulting biomaterials can offer the interesting properties associated with NO, like antimicrobial activity as preliminary tests showed here with Escherichia coli and Staphylococcus epidermidis.					
31923961	0	18	theme	nitric	95:100	arg1	oxide					102:106	nitric oxide	95:106	nitric oxide generation	95:117	Cellulose-based biomaterials integrated with copper-cystine hybrid structures as catalysts for nitric oxide generation.					
31923961	1	19	theme	copper-cystine	198:211	arg1	structures					231:240	polymer-coated copper-cystine high-aspect ratio structures	183:240	polymer-coated copper-cystine high-aspect ratio structures (CuHARS)	183:249	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	1	19	theme	copper-cystine	198:211	arg1	CuHARS					243:248	CuHARS	243:248	CuHARS	243:248	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	10	20	theme	brain	2065:2069	arg1	BMVECs					2090:2095	BMVECs	2090:2095	BMVECs	2090:2095	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	20	theme	brain	2065:2069	arg1	cells					2083:2087	rat brain endothelial cells	2061:2087	rat brain endothelial cells (BMVECs)	2061:2096	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	5	21	theme	biomedical	1049:1058	arg1	applications					1060:1071	potential biomedical applications	1039:1071	potential biomedical applications as implants or wound dressing materials	1039:1111	Based on these characteristics, the cellulose/PAH-CuHARS composites are promising for potential biomedical applications as implants or wound dressing materials.					
31923961	9	22	theme	bactericidal	1823:1834	arg1	effect					1836:1841	bactericidal effect	1823:1841	bactericidal effect on the studied bacteria	1823:1865	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	4	23	theme	initial	817:823	arg1	suspension					825:834	the initial suspension	813:834	the initial suspension	813:834	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	7	24	theme	unit	1386:1389	arg1	structure					1353:1361	the crystalline structure	1337:1361	the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood	1337:1500	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	0	25	theme	Cellulose-based	0:14	arg1	biomaterials					16:27	Cellulose-based biomaterials	0:27	Cellulose-based biomaterials	0:27	Cellulose-based biomaterials integrated with copper-cystine hybrid structures as catalysts for nitric oxide generation.					
31923961	10	26	from	effect	2029:2034	arg1	BMVECs					2090:2095	BMVECs	2090:2095	BMVECs	2090:2095	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	26	from	effect	2029:2034	arg1	cells					2083:2087	rat brain endothelial cells	2061:2087	rat brain endothelial cells (BMVECs)	2061:2096	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	9	27	theme	physiological	1721:1733	arg1	concentration					1735:1747	physiological concentration	1721:1747	physiological concentration of RSNOs	1721:1756	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	7	28	theme	CuHARS	1370:1375	arg1	unit					1386:1389	the CuHARS building unit	1366:1389	the CuHARS building unit	1366:1389	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	9	29	theme	studied	1850:1856	arg1	bacteria					1858:1865	the studied bacteria	1846:1865	the studied bacteria	1846:1865	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	4	30	theme	Young	604:608	arg1	moduli					612:617	Young's moduli	604:617	Young's moduli around 1.5 GPa	604:632	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	3	31	theme	solvent	509:515	arg1	casting					517:523	solvent casting	509:523	solvent casting	509:523	The resulting materials can be processed as films or macroporous foams by solvent casting and lyophilization, respectively.					
31923961	8	32	theme	antimicrobial	1592:1604	arg1	activity					1606:1613	antimicrobial activity	1592:1613	antimicrobial activity	1592:1613	The resulting biomaterials can offer the interesting properties associated with NO, like antimicrobial activity as preliminary tests showed here with Escherichia coli and Staphylococcus epidermidis.					
31923961	0	33	theme	copper-cystine	45:58	arg1	structures					67:76	copper-cystine hybrid structures	45:76	copper-cystine hybrid structures as catalysts for nitric oxide generation	45:117	Cellulose-based biomaterials integrated with copper-cystine hybrid structures as catalysts for nitric oxide generation.					
31923961	4	34	dep	show	569:572	arg1	while					666:670	while	666:670	while	666:670	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	4	35	from	behavior	590:597	arg1	water					659:663	water	659:663	water	659:663	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	7	36	theme	crystalline	1341:1351	arg1	structure					1353:1361	the crystalline structure	1337:1361	the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood	1337:1500	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	4	37	theme	good	574:577	arg1	behavior					590:597	good mechanical behavior	574:597	good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water	574:663	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	2	38	theme	TEMPO-oxidized	389:402	arg1	cellulose					404:412	TEMPO-oxidized cellulose	389:412	TEMPO-oxidized cellulose	389:412	The coating of the metal-organic materials with polyallylamine hydrochloride (PAH) allows their covalent linkage to TEMPO-oxidized cellulose by means of EDC/NHS.					
31923961	9	39	dep	concentration	1735:1747	arg1	presence					1709:1716	presence	1709:1716	presence	1709:1716	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	9	39	dep	concentration	1735:1747	arg1	the					1705:1707	the	1705:1707	the	1705:1707	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	7	40	from	structure	1353:1361	arg1	presence					1315:1322	the presence	1311:1322	the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood	1311:1500	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	6	41	theme	nitric	1263:1268	arg1	NO					1277:1278	NO	1277:1278	NO	1277:1278	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	6	41	theme	nitric	1263:1268	arg1	oxide					1270:1274	nitric oxide	1263:1274	nitric oxide (NO)	1263:1279	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	1	42	theme	fibers	265:270	arg1	assembly					171:178	the assembly	167:178	the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers	167:270	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	4	43	theme	compression	841:851	arg1	values					861:866	compression modulus values	841:866	compression modulus values	841:866	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	5	44	theme	dressing	1094:1101	arg1	materials					1103:1111	wound dressing materials	1088:1111	wound dressing materials	1088:1111	Based on these characteristics, the cellulose/PAH-CuHARS composites are promising for potential biomedical applications as implants or wound dressing materials.					
31923961	6	45	theme	weight	1185:1190	arg1	RSNOs					1209:1213	RSNOs	1209:1213	RSNOs	1209:1213	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	6	45	theme	weight	1185:1190	arg1	S-nitrosothiols					1192:1206	low molecular weight S-nitrosothiols	1171:1206	low molecular weight S-nitrosothiols (RSNOs)	1171:1214	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	4	46	theme	reported	901:908	arg1	aerogels					943:950	other reported freeze-dried nanocellulose-based aerogels	895:950	other reported freeze-dried nanocellulose-based aerogels	895:950	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	10	47	dep	significant	2107:2117	arg1	significant					2107:2117	significant	2107:2117	significant	2107:2117	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	47	dep	significant	2107:2117	arg1	effect					2029:2034	The cytotoxic effect	2015:2034	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs)	2015:2096	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	47	dep	significant	2107:2117	arg1	least					2101:2105	least	2101:2105	least	2101:2105	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	6	48	theme	low	1171:1173	arg1	weight					1185:1190	low molecular weight	1171:1190	low molecular weight S-nitrosothiols (RSNOs)	1171:1214	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	4	49	theme	nanocellulose-based	923:941	arg1	aerogels					943:950	other reported freeze-dried nanocellulose-based aerogels	895:950	other reported freeze-dried nanocellulose-based aerogels	895:950	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	9	50	theme	low	1929:1931	arg1	concentration					1933:1945	low concentration	1929:1945	low concentration in the nM range like anti-inflammatory and anti-thrombotic effects	1929:2012	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	4	51	with	macropores	730:739	arg1	diameters					754:762	average diameters	746:762	average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels	746:950	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	6	52	from	decomposition	1154:1166	arg1	effective					1137:1145	effective	1137:1145	effective	1137:1145	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	2	53	theme	polyallylamine	321:334	arg1	hydrochloride					336:348	polyallylamine hydrochloride	321:348	polyallylamine hydrochloride (PAH)	321:354	The coating of the metal-organic materials with polyallylamine hydrochloride (PAH) allows their covalent linkage to TEMPO-oxidized cellulose by means of EDC/NHS.					
31923961	2	53	theme	polyallylamine	321:334	arg1	PAH					351:353	PAH	351:353	PAH	351:353	The coating of the metal-organic materials with polyallylamine hydrochloride (PAH) allows their covalent linkage to TEMPO-oxidized cellulose by means of EDC/NHS.					
31923961	1	54	theme	high-aspect	213:223	arg1	structures					231:240	polymer-coated copper-cystine high-aspect ratio structures	183:240	polymer-coated copper-cystine high-aspect ratio structures (CuHARS)	183:249	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	1	54	theme	high-aspect	213:223	arg1	CuHARS					243:248	CuHARS	243:248	CuHARS	243:248	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	7	55	from	presence	1315:1322	arg1	structure					1353:1361	the crystalline structure	1337:1361	the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood	1337:1500	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	7	56	theme	ascorbic	1462:1469	arg1	acid					1471:1474	ascorbic acid	1462:1474	ascorbic acid	1462:1474	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	4	57	from	resistance	645:654	arg1	water					659:663	water	659:663	water	659:663	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	1	58	theme	structures	231:240	arg1	assembly					171:178	the assembly	167:178	the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers	167:270	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	9	59	theme	RSNOs	1752:1756	arg1	concentration					1735:1747	physiological concentration	1721:1747	physiological concentration of RSNOs	1721:1756	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	7	60	from	copper	1327:1332	arg1	structure					1353:1361	the crystalline structure	1337:1361	the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood	1337:1500	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	7	61	theme	species	1449:1455	arg1	presence					1431:1438	the presence	1427:1438	the presence of redox species like ascorbic acid, typically found in blood	1427:1500	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	9	62	theme	nM	1954:1955	arg1	range					1957:1961	the nM range	1950:1961	the nM range like anti-inflammatory and anti-thrombotic effects	1950:2012	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	4	63	theme	open	699:702	arg1	network					704:710	an open network	696:710	an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels	696:950	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	6	64	theme	similar	1217:1223	arg1	RSNOs					1209:1213	RSNOs	1209:1213	RSNOs	1209:1213	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	6	64	theme	similar	1217:1223	arg1	S-nitrosothiols					1192:1206	low molecular weight S-nitrosothiols	1171:1206	low molecular weight S-nitrosothiols (RSNOs)	1171:1214	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	10	65	theme	biological	2169:2178	arg1	studies					2180:2186	further biological studies	2161:2186	further biological studies	2161:2186	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	9	66	theme	generated	1772:1780	arg1	NO					1782:1783	generated NO	1772:1783	generated NO (around 360 nM)	1772:1799	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	4	67	theme	obtained	676:683	arg1	foams					685:689	the obtained foams	672:689	the obtained foams	672:689	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	2	68	with	materials	306:314	arg1	hydrochloride					336:348	polyallylamine hydrochloride	321:348	polyallylamine hydrochloride (PAH)	321:354	The coating of the metal-organic materials with polyallylamine hydrochloride (PAH) allows their covalent linkage to TEMPO-oxidized cellulose by means of EDC/NHS.					
31923961	2	68	with	materials	306:314	arg1	PAH					351:353	PAH	351:353	PAH	351:353	The coating of the metal-organic materials with polyallylamine hydrochloride (PAH) allows their covalent linkage to TEMPO-oxidized cellulose by means of EDC/NHS.					
31923961	4	69	theme	average	746:752	arg1	diameters					754:762	average diameters	746:762	average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels	746:950	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	2	70	theme	materials	306:314	arg1	coating					277:283	The coating	273:283	The coating of the metal-organic materials with polyallylamine hydrochloride (PAH)	273:354	The coating of the metal-organic materials with polyallylamine hydrochloride (PAH) allows their covalent linkage to TEMPO-oxidized cellulose by means of EDC/NHS.					
31923961	7	71	located	found	1487:1491	arg1	blood					1496:1500	blood	1496:1500	blood	1496:1500	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	7	71	located	found	1487:1491	arg2	species					1449:1455	redox species	1443:1455	redox species	1443:1455	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	10	72	theme	films	2052:2056	arg1	significant					2107:2117	significant	2107:2117	significant	2107:2117	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	72	theme	films	2052:2056	arg1	effect					2029:2034	The cytotoxic effect	2015:2034	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs)	2015:2096	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	72	theme	films	2052:2056	arg1	least					2101:2105	least	2101:2105	least	2101:2105	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	9	73	from	concentration	1933:1945	arg1	range					1957:1961	the nM range	1950:1961	the nM range like anti-inflammatory and anti-thrombotic effects	1950:2012	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	0	74	theme	oxide	102:106	arg1	generation					108:117	nitric oxide generation	95:117	nitric oxide generation	95:117	Cellulose-based biomaterials integrated with copper-cystine hybrid structures as catalysts for nitric oxide generation.					
31923961	1	75	theme	polymer-coated	183:196	arg1	structures					231:240	polymer-coated copper-cystine high-aspect ratio structures	183:240	polymer-coated copper-cystine high-aspect ratio structures (CuHARS)	183:249	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	1	75	theme	polymer-coated	183:196	arg1	CuHARS					243:248	CuHARS	243:248	CuHARS	243:248	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	8	76	theme	interesting	1544:1554	arg1	tests					1630:1634	preliminary tests	1618:1634	preliminary tests showed here with Escherichia coli and Staphylococcus epidermidis	1618:1699	The resulting biomaterials can offer the interesting properties associated with NO, like antimicrobial activity as preliminary tests showed here with Escherichia coli and Staphylococcus epidermidis.					
31923961	8	76	theme	interesting	1544:1554	arg1	properties					1556:1565	the interesting properties	1540:1565	the interesting properties	1540:1565	The resulting biomaterials can offer the interesting properties associated with NO, like antimicrobial activity as preliminary tests showed here with Escherichia coli and Staphylococcus epidermidis.					
31923961	4	77	theme	macropores	730:739	arg1	network					704:710	an open network	696:710	an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels	696:950	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	10	78	theme	endothelial	2071:2081	arg1	BMVECs					2090:2095	BMVECs	2090:2095	BMVECs	2090:2095	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	78	theme	endothelial	2071:2081	arg1	cells					2083:2087	rat brain endothelial cells	2061:2087	rat brain endothelial cells (BMVECs)	2061:2096	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	5	79	theme	potential	1039:1047	arg1	applications					1060:1071	potential biomedical applications	1039:1071	potential biomedical applications as implants or wound dressing materials	1039:1111	Based on these characteristics, the cellulose/PAH-CuHARS composites are promising for potential biomedical applications as implants or wound dressing materials.					
31923961	10	80	theme	rat	2061:2063	arg1	BMVECs					2090:2095	BMVECs	2090:2095	BMVECs	2090:2095	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	10	80	theme	rat	2061:2063	arg1	cells					2083:2087	rat brain endothelial cells	2061:2087	rat brain endothelial cells (BMVECs)	2061:2096	The cytotoxic effect recorded of the films on rat brain endothelial cells (BMVECs) is least significant and proves them to be friendly enough for further biological studies.					
31923961	7	81	theme	building	1377:1384	arg1	unit					1386:1389	the CuHARS building unit	1366:1389	the CuHARS building unit	1366:1389	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	3	82	theme	macroporous	488:498	arg1	materials					449:457	The resulting materials	435:457	The resulting materials	435:457	The resulting materials can be processed as films or macroporous foams by solvent casting and lyophilization, respectively.					
31923961	3	82	theme	macroporous	488:498	arg1	foams					500:504	macroporous foams	488:504	macroporous foams	488:504	The resulting materials can be processed as films or macroporous foams by solvent casting and lyophilization, respectively.					
31923961	3	83	theme	resulting	439:447	arg1	foams					500:504	macroporous foams	488:504	macroporous foams	488:504	The resulting materials can be processed as films or macroporous foams by solvent casting and lyophilization, respectively.					
31923961	3	83	theme	resulting	439:447	arg1	films					479:483	films	479:483	films	479:483	The resulting materials can be processed as films or macroporous foams by solvent casting and lyophilization, respectively.					
31923961	3	83	theme	resulting	439:447	arg1	materials					449:457	The resulting materials	435:457	The resulting materials	435:457	The resulting materials can be processed as films or macroporous foams by solvent casting and lyophilization, respectively.					
31923961	1	84	theme	Bionanocomposite	120:135	arg1	materials					137:145	Bionanocomposite materials	120:145	Bionanocomposite materials	120:145	Bionanocomposite materials were developed from the assembly of polymer-coated copper-cystine high-aspect ratio structures (CuHARS) and cellulose fibers.					
31923961	0	85	theme	hybrid	60:65	arg1	structures					67:76	copper-cystine hybrid structures	45:76	copper-cystine hybrid structures as catalysts for nitric oxide generation	45:117	Cellulose-based biomaterials integrated with copper-cystine hybrid structures as catalysts for nitric oxide generation.					
31923961	9	86	from	effect	1836:1841	arg1	bacteria					1858:1865	the studied bacteria	1846:1865	the studied bacteria	1846:1865	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	4	87	theme	mechanical	579:588	arg1	behavior					590:597	good mechanical behavior	574:597	good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water	574:663	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	4	88	theme	modulus	853:859	arg1	values					861:866	compression modulus values	841:866	compression modulus values	841:866	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	7	89	theme	copper	1327:1332	arg1	presence					1315:1322	the presence	1311:1322	the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood	1311:1500	This effect is attributed to the presence of copper in the crystalline structure of the CuHARS building unit, which can be gradually released in the presence of redox species like ascorbic acid, typically found in blood.					
31923961	9	90	theme	other	1889:1893	arg1	properties					1895:1904	other properties	1889:1904	other properties inherent to NO	1889:1919	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31923961	4	91	theme	suspension	825:834	arg1	concentration					796:808	the concentration	792:808	the concentration of the initial suspension	792:834	The films show good mechanical behavior with Young's moduli around 1.5 GPa as well as resistance in water, while the obtained foams show an open network of interconnected macropores with average diameters around 130 μm, depending on the concentration of the initial suspension, and compression modulus values around 450 kPa, similar to other reported freeze-dried nanocellulose-based aerogels.					
31923961	2	92	theme	covalent	369:376	arg1	linkage					378:384	their covalent linkage	363:384	their covalent linkage to TEMPO-oxidized cellulose	363:412	The coating of the metal-organic materials with polyallylamine hydrochloride (PAH) allows their covalent linkage to TEMPO-oxidized cellulose by means of EDC/NHS.					
31923961	5	93	theme	wound	1088:1092	arg1	materials					1103:1111	wound dressing materials	1088:1111	wound dressing materials	1088:1111	Based on these characteristics, the cellulose/PAH-CuHARS composites are promising for potential biomedical applications as implants or wound dressing materials.					
31923961	6	94	theme	S-nitrosothiols	1192:1206	arg1	decomposition					1154:1166	the decomposition	1150:1166	the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO)	1150:1279	They have proved to be effective in the decomposition of low molecular weight S-nitrosothiols (RSNOs), similar to those existing in blood, releasing nitric oxide (NO).					
31923961	9	95	theme	inherent	1906:1913	arg1	properties					1895:1904	other properties	1889:1904	other properties inherent to NO	1889:1919	In the presence of physiological concentration of RSNOs the amount of generated NO (around 360 nM) is not enough to show bactericidal effect on the studied bacteria, but it could provide other properties inherent to NO even at low concentration in the nM range like anti-inflammatory and anti-thrombotic effects.					
31687407	1	0	theme	animal	325:330	arg1	models					332:337	animal models	325:337	animal models	325:337	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	2	1	theme	konjac	652:657	arg1	group					645:649	konjac extract group	630:649	konjac extract group (konjac group)	630:664	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	1	theme	konjac	652:657	arg1	group					659:663	konjac group	652:663	konjac group	652:663	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	2	theme	inulin	693:698	arg1	group					700:704	inulin group	693:704	inulin group	693:704	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	2	theme	inulin	693:698	arg1	group					610:614	high dose group	600:614	high dose group (KI-H group)	600:627	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	3	theme	inulin	1372:1377	arg1	group					1365:1369	inulin extract group	1350:1369	inulin extract group (inulin group)	1350:1384	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	3	theme	inulin	1372:1377	arg1	group					1379:1383	inulin group	1372:1383	inulin group	1372:1383	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	13	4	theme	high-risk	2145:2153	arg1	population					2155:2164	diabetic or diabetic high-risk population	2124:2164	population	2155:2164	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	2	5	theme	KI	485:486	arg1	combination					488:498	KI combination	485:498	KI combination at low dose group (KI-L group)	485:529	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	5	theme	KI	485:486	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	6	6	theme	plasma	928:933	arg1	proteins					983:990	glycated serum proteins	968:990	glycated serum proteins	968:990	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	6	6	theme	plasma	928:933	arg1	cholesterol					999:1009	total cholesterol	993:1009	total cholesterol	993:1009	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	6	6	theme	plasma	928:933	arg1	triglycerides					1016:1028	triglycerides	1016:1028	triglycerides	1016:1028	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	6	6	theme	plasma	928:933	arg1	indicators					947:956	plasma biochemical indicators	928:956	plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides	928:1028	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	1	7	theme	diet	226:229	arg1	supplementary					231:243	as diet supplementary	223:243	as diet supplementary	223:243	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	1	7	theme	diet	226:229	arg1	combination					178:188	their combination	172:188	their combination	172:188	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	7	8	theme	db/db	1180:1184	arg1	mice					1186:1189	db/db mice	1180:1189	db/db mice	1180:1189	Type 2 diabetes was developed in db/db mice.					
31687407	2	9	theme	high	600:603	arg1	group					700:704	inulin group	693:704	inulin group	693:704	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	9	theme	high	600:603	arg1	group					610:614	high dose group	600:614	high dose group (KI-H group)	600:627	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	9	theme	high	600:603	arg1	group					622:626	KI-H group	617:626	KI-H group	617:626	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	10	from	group	562:566	arg1	combination					535:545	KI combination	532:545	KI combination at medium dose group (KI-M group)	532:579	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	10	from	group	562:566	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	13	11	theme	diabetic	2124:2131	arg1	population					2155:2164	diabetic or diabetic high-risk population	2124:2164	population	2155:2164	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	11	12	theme	blood	1648:1652	arg1	triglycerides					1654:1666	blood triglycerides	1648:1666	blood triglycerides	1648:1666	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	2	13	theme	dose	507:510	arg1	group					512:516	low dose group	503:516	low dose group (KI-L group)	503:529	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	13	theme	dose	507:510	arg1	group					524:528	KI-L group	519:528	KI-L group	519:528	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	13	14	theme	diabetic	2136:2143	arg1	population					2155:2164	diabetic or diabetic high-risk population	2124:2164	population	2155:2164	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	13	15	theme	composition	1966:1976	arg1	use					1956:1958	daily use	1950:1958	daily use of KI composition	1950:1976	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	2	16	theme	KI-H	617:620	arg1	group					610:614	high dose group	600:614	high dose group (KI-H group)	600:627	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	16	theme	KI-H	617:620	arg1	group					622:626	KI-H group	617:626	KI-H group	617:626	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	11	17	theme	diabetic	1771:1778	arg1	rats					1780:1783	STZ-induced diabetic rats	1759:1783	STZ-induced diabetic rats	1759:1783	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	6	18	theme	total	993:997	arg1	cholesterol					999:1009	total cholesterol	993:1009	total cholesterol	993:1009	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	6	19	theme	pancreas	1089:1096	arg1	staining					1069:1076	immunohistochemistry staining	1048:1076	immunohistochemistry staining of the rat pancreas	1048:1096	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	13	20	theme	daily	1950:1954	arg1	use					1956:1958	daily use	1950:1958	daily use of KI composition	1950:1976	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	9	21	theme	wild-type	1389:1397	arg1	group					1407:1411	A wild-type control group	1387:1411	A wild-type control group (wild-type group) for db/db mice	1387:1444	A wild-type control group (wild-type group) for db/db mice was also included.					
31687407	9	21	theme	wild-type	1389:1397	arg1	group					1424:1428	wild-type group	1414:1428	wild-type group	1414:1428	A wild-type control group (wild-type group) for db/db mice was also included.					
31687407	11	22	theme	composition	1584:1594	arg1	use					1570:1572	RESULTS Daily use	1556:1572	RESULTS Daily use of the KI composition	1556:1594	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	1	23	theme	present	94:100	arg1	study					102:106	The present study	90:106	The present study	90:106	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	2	24	theme	STZ	473:475	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	24	theme	STZ	473:475	arg1	group					477:481	STZ group	473:481	STZ group	473:481	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	25	theme	mice	1212:1215	arg1	total					1194:1198	A total	1192:1198	A total of 28 db/db mice	1192:1215	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	6	26	theme	glycated	968:975	arg1	proteins					983:990	glycated serum proteins	968:990	glycated serum proteins	968:990	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	2	27	theme	dose	557:560	arg1	group					562:566	medium dose group	550:566	medium dose group (KI-M group)	550:579	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	27	theme	dose	557:560	arg1	group					574:578	KI-M group	569:578	KI-M group	569:578	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	13	28	dep	CONCLUSION	1900:1909	arg1	suggest					1937:1943	suggest	1937:1943	suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population	1937:2164	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	13	29	theme	composition	2087:2097	arg1	development					2069:2079	further development	2061:2079	further development of KI composition	2061:2097	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	11	30	theme	glucose	1636:1642	arg1	levels					1620:1625	levels	1620:1625	levels of blood glucose and blood triglycerides	1620:1666	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	9	31	theme	wild-type	1414:1422	arg1	group					1407:1411	A wild-type control group	1387:1411	A wild-type control group (wild-type group) for db/db mice	1387:1444	A wild-type control group (wild-type group) for db/db mice was also included.					
31687407	9	31	theme	wild-type	1414:1422	arg1	group					1424:1428	wild-type group	1414:1428	wild-type group	1414:1428	A wild-type control group (wild-type group) for db/db mice was also included.					
31687407	8	32	dep	groups	1237:1242	arg1	control					1253:1259	vehicle control	1245:1259	4 groups: vehicle control (db/db group)	1235:1273	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	32	dep	groups	1237:1242	arg1	group					1268:1272	db/db group	1262:1272	db/db group	1262:1272	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	5	33	theme	tolerance	870:878	arg1	test					880:883	a glucose tolerance test	860:883	a glucose tolerance test	860:883	After continuous treatment of each diet for 24 days, a glucose tolerance test was performed.					
31687407	10	34	theme	blood	1475:1479	arg1	glucose					1481:1487	blood glucose	1475:1487	blood glucose	1475:1487	Levels of blood glucose, body weight, and blood triglycerides were monitored at each week.					
31687407	2	35	from	group	686:690	arg1	combination					585:595	KI combination	582:595	KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group)	582:705	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	35	from	group	686:690	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	9	36	theme	db/db	1435:1439	arg1	mice					1441:1444	db/db mice	1435:1444	db/db mice	1435:1444	A wild-type control group (wild-type group) for db/db mice was also included.					
31687407	12	37	theme	inulin	1884:1889	arg1	extract					1891:1897	inulin extract	1884:1897	inulin extract	1884:1897	Such effects from KI composition were better than single ingredient of konjac or inulin extract.					
31687407	2	38	theme	KI-L	519:522	arg1	group					512:516	low dose group	503:516	low dose group (KI-L group)	503:529	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	38	theme	KI-L	519:522	arg1	group					524:528	KI-L group	519:528	KI-L group	519:528	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	39	theme	extract	678:684	arg1	group					686:690	inulin extract group	671:690	inulin extract group	671:690	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	13	40	theme	further	2061:2067	arg1	development					2069:2079	further development	2061:2079	further development of KI composition	2061:2097	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	8	41	theme	db/db	1262:1266	arg1	control					1253:1259	vehicle control	1245:1259	4 groups: vehicle control (db/db group)	1235:1273	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	41	theme	db/db	1262:1266	arg1	group					1268:1272	db/db group	1262:1272	db/db group	1262:1272	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	0	42	theme	Protective	0:9	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes	0:77	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.					
31687407	11	43	theme	db/db	1791:1795	arg1	mice					1797:1800	db/db mice	1791:1800	db/db mice	1791:1800	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	2	44	theme	KI	532:533	arg1	combination					535:545	KI combination	532:545	KI combination at medium dose group (KI-M group)	532:579	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	44	theme	KI	532:533	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	12	45	theme	single	1853:1858	arg1	ingredient					1860:1869	single ingredient	1853:1869	single ingredient of konjac or inulin extract	1853:1897	Such effects from KI composition were better than single ingredient of konjac or inulin extract.					
31687407	2	46	theme	diabetic	362:369	arg1	rats					380:383	60 diabetic (type 1) rats	359:383	60 diabetic (type 1) rats induced by streptozotocin (STZ)	359:415	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	6	47	theme	treatment	917:925	arg1	days					909:912	28 days	906:912	28 days of treatment	906:925	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	3	48	theme	sham	710:713	arg1	group					715:719	A sham group	708:719	A sham group (without STZ)	708:733	A sham group (without STZ) was also included.					
31687407	4	49	theme	glucose	770:776	arg1	Levels					754:759	Levels	754:759	Levels of blood glucose	754:776	Levels of blood glucose were monitored at each week.					
31687407	8	50	theme	KI	1276:1277	arg1	groups					1237:1242	4 groups	1235:1242	4 groups: vehicle control (db/db group)	1235:1273	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	50	theme	KI	1276:1277	arg1	group					1291:1295	KI composition group	1276:1295	KI composition group (KI group)	1276:1306	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	50	theme	KI	1276:1277	arg1	group					1301:1305	KI group	1298:1305	KI group	1298:1305	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	11	51	theme	Daily	1564:1568	arg1	use					1570:1572	RESULTS Daily use	1556:1572	RESULTS Daily use of the KI composition	1556:1594	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	1	52	theme	beneficial	256:265	arg1	effects					267:273	beneficial effects	256:273	beneficial effects	256:273	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	10	53	theme	weight	1495:1500	arg1	Levels					1465:1470	Levels	1465:1470	Levels of blood glucose, body weight, and blood triglycerides	1465:1525	Levels of blood glucose, body weight, and blood triglycerides were monitored at each week.					
31687407	2	54	dep	diabetic	362:369	arg1	type					372:375	type 1	372:377	type 1	372:377	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	55	theme	extract	637:643	arg1	group					645:649	konjac extract group	630:649	konjac extract group (konjac group)	630:664	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	55	theme	extract	637:643	arg1	group					659:663	konjac group	652:663	konjac group	652:663	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	56	theme	KI	1298:1299	arg1	group					1291:1295	KI composition group	1276:1295	KI composition group (KI group)	1276:1306	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	56	theme	KI	1298:1299	arg1	group					1301:1305	KI group	1298:1305	KI group	1298:1305	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	1	57	theme	konjac-inulin	191:203	arg1	combination					178:188	their combination	172:188	their combination	172:188	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	1	57	theme	konjac-inulin	191:203	arg1	composition					210:220	konjac-inulin (KI) composition	191:220	konjac-inulin (KI) composition	191:220	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	10	58	theme	triglycerides	1513:1525	arg1	Levels					1465:1470	Levels	1465:1470	Levels of blood glucose, body weight, and blood triglycerides	1465:1525	Levels of blood glucose, body weight, and blood triglycerides were monitored at each week.					
31687407	12	59	theme	Such	1803:1806	arg1	effects					1808:1814	Such effects	1803:1814	Such effects from KI composition	1803:1834	Such effects from KI composition were better than single ingredient of konjac or inulin extract.					
31687407	1	60	theme	KI	206:207	arg1	combination					178:188	their combination	172:188	their combination	172:188	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	1	60	theme	KI	206:207	arg1	composition					210:220	konjac-inulin (KI) composition	191:220	konjac-inulin (KI) composition	191:220	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	8	61	theme	konjac	1309:1314	arg1	groups					1237:1242	4 groups	1235:1242	4 groups: vehicle control (db/db group)	1235:1273	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	61	theme	konjac	1309:1314	arg1	group					1324:1328	konjac extract group	1309:1328	konjac extract group (konjac group)	1309:1343	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	61	theme	konjac	1309:1314	arg1	group					1338:1342	konjac group	1331:1342	konjac group	1331:1342	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	6	62	theme	immunohistochemistry	1048:1067	arg1	staining					1069:1076	immunohistochemistry staining	1048:1076	immunohistochemistry staining of the rat pancreas	1048:1096	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	8	63	theme	extract	1357:1363	arg1	group					1365:1369	inulin extract group	1350:1369	inulin extract group (inulin group)	1350:1384	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	63	theme	extract	1357:1363	arg1	group					1379:1383	inulin group	1372:1383	inulin group	1372:1383	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	63	theme	extract	1357:1363	arg1	groups					1237:1242	4 groups	1235:1242	4 groups: vehicle control (db/db group)	1235:1273	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	11	64	theme	STZ-induced	1759:1769	arg1	rats					1780:1783	STZ-induced diabetic rats	1759:1783	STZ-induced diabetic rats	1759:1783	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	11	65	theme	triglycerides	1654:1666	arg1	levels					1620:1625	levels	1620:1625	levels of blood glucose and blood triglycerides	1620:1666	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	1	66	theme	as	223:224	arg1	supplementary					231:243	as diet supplementary	223:243	as diet supplementary	223:243	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	1	66	theme	as	223:224	arg1	combination					178:188	their combination	172:188	their combination	172:188	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	2	67	dep	groups	448:453	arg1	combination					488:498	KI combination	485:498	KI combination at low dose group (KI-L group)	485:529	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	67	dep	groups	448:453	arg1	combination					535:545	KI combination	532:545	KI combination at medium dose group (KI-M group)	532:579	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	67	dep	groups	448:453	arg1	combination					585:595	KI combination	582:595	KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group)	582:705	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	67	dep	groups	448:453	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	67	dep	groups	448:453	arg1	group					477:481	STZ group	473:481	STZ group	473:481	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	11	68	theme	obesity	1748:1754	arg1	development					1733:1743	development	1733:1743	development of obesity	1733:1754	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	2	69	theme	dose	605:608	arg1	group					700:704	inulin group	693:704	inulin group	693:704	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	69	theme	dose	605:608	arg1	group					610:614	high dose group	600:614	high dose group (KI-H group)	600:627	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	69	theme	dose	605:608	arg1	group					622:626	KI-H group	617:626	KI-H group	617:626	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	70	theme	konjac	1331:1336	arg1	group					1324:1328	konjac extract group	1309:1328	konjac extract group (konjac group)	1309:1343	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	70	theme	konjac	1331:1336	arg1	group					1338:1342	konjac group	1331:1342	konjac group	1331:1342	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	13	71	theme	KI	1963:1964	arg1	composition					1966:1976	KI composition	1963:1976	KI composition	1963:1976	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	1	72	theme	konjac	142:147	arg1	extracts					160:167	konjac and inulin extracts	142:167	konjac and inulin extracts	142:167	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	13	73	contain	has	1978:1980	arg2	role					1995:1998	a protective role	1982:1998	a protective role	1982:1998	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	13	73	contain	has	1978:1980	arg1	use					1956:1958	daily use	1950:1958	daily use of KI composition	1950:1976	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	1	74	theme	inulin	153:158	arg1	extracts					160:167	konjac and inulin extracts	142:167	konjac and inulin extracts	142:167	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	8	75	theme	vehicle	1245:1251	arg1	control					1253:1259	vehicle control	1245:1259	4 groups: vehicle control (db/db group)	1235:1273	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	75	theme	vehicle	1245:1251	arg1	group					1268:1272	db/db group	1262:1272	db/db group	1262:1272	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	76	theme	low	503:505	arg1	group					512:516	low dose group	503:516	low dose group (KI-L group)	503:529	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	76	theme	low	503:505	arg1	group					524:528	KI-L group	519:528	KI-L group	519:528	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	13	77	theme	study	1931:1935	arg1	results					1915:1921	The results	1911:1921	The results of this study	1911:1935	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	2	78	theme	vehicle	456:462	arg1	combination					488:498	KI combination	485:498	KI combination at low dose group (KI-L group)	485:529	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	78	theme	vehicle	456:462	arg1	combination					535:545	KI combination	532:545	KI combination at medium dose group (KI-M group)	532:579	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	78	theme	vehicle	456:462	arg1	combination					585:595	KI combination	582:595	KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group)	582:705	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	78	theme	vehicle	456:462	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	78	theme	vehicle	456:462	arg1	group					477:481	STZ group	473:481	STZ group	473:481	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	13	79	theme	food	2104:2107	arg1	basis					2051:2055	experimental basis	2038:2055	experimental basis for further development of KI composition	2038:2097	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	13	79	theme	food	2104:2107	arg1	supplement					2109:2118	a food supplement	2102:2118	a food supplement for diabetic or diabetic high-risk population	2102:2164	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	5	80	theme	diet	842:845	arg1	treatment					824:832	continuous treatment	813:832	continuous treatment of each diet for 24 days	813:857	After continuous treatment of each diet for 24 days, a glucose tolerance test was performed.					
31687407	11	81	theme	insulin	1693:1699	arg1	production					1701:1710	the insulin production	1689:1710	the insulin production	1689:1710	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	8	82	theme	db/db	1206:1210	arg1	mice					1212:1215	28 db/db mice	1203:1215	28 db/db mice	1203:1215	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	83	theme	KI-M	569:572	arg1	group					562:566	medium dose group	550:566	medium dose group (KI-M group)	550:579	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	83	theme	KI-M	569:572	arg1	group					574:578	KI-M group	569:578	KI-M group	569:578	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	84	from	group	512:516	arg1	combination					488:498	KI combination	485:498	KI combination at low dose group (KI-L group)	485:529	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	84	from	group	512:516	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	11	85	theme	blood	1630:1634	arg1	glucose					1636:1642	blood glucose	1630:1642	blood glucose	1630:1642	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	6	86	theme	rat	1085:1087	arg1	pancreas					1089:1096	the rat pancreas	1081:1096	the rat pancreas	1081:1096	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	11	87	theme	KI	1581:1582	arg1	composition					1584:1594	the KI composition	1577:1594	the KI composition	1577:1594	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	9	88	theme	control	1399:1405	arg1	group					1407:1411	A wild-type control group	1387:1411	A wild-type control group (wild-type group) for db/db mice	1387:1444	A wild-type control group (wild-type group) for db/db mice was also included.					
31687407	9	88	theme	control	1399:1405	arg1	group					1424:1428	wild-type group	1414:1428	wild-type group	1414:1428	A wild-type control group (wild-type group) for db/db mice was also included.					
31687407	6	89	theme	serum	977:981	arg1	proteins					983:990	glycated serum proteins	968:990	glycated serum proteins	968:990	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	13	90	theme	KI	2084:2085	arg1	composition					2087:2097	KI composition	2084:2097	KI composition	2084:2097	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	5	91	theme	continuous	813:822	arg1	treatment					824:832	continuous treatment	813:832	continuous treatment of each diet for 24 days	813:857	After continuous treatment of each diet for 24 days, a glucose tolerance test was performed.					
31687407	10	92	theme	body	1490:1493	arg1	weight					1495:1500	body weight	1490:1500	body weight	1490:1500	Levels of blood glucose, body weight, and blood triglycerides were monitored at each week.					
31687407	2	93	theme	KI	582:583	arg1	combination					585:595	KI combination	582:595	KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group)	582:705	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	93	theme	KI	582:583	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	10	94	theme	glucose	1481:1487	arg1	Levels					1465:1470	Levels	1465:1470	Levels of blood glucose, body weight, and blood triglycerides	1465:1525	Levels of blood glucose, body weight, and blood triglycerides were monitored at each week.					
31687407	6	95	theme	biochemical	935:945	arg1	proteins					983:990	glycated serum proteins	968:990	glycated serum proteins	968:990	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	6	95	theme	biochemical	935:945	arg1	cholesterol					999:1009	total cholesterol	993:1009	total cholesterol	993:1009	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	6	95	theme	biochemical	935:945	arg1	triglycerides					1016:1028	triglycerides	1016:1028	triglycerides	1016:1028	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	6	95	theme	biochemical	935:945	arg1	indicators					947:956	plasma biochemical indicators	928:956	plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides	928:1028	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	1	96	dep	OBJECTIVE	80:88	arg1	designed					112:119	designed	112:119	was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models	108:337	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	0	97	theme	Type	63:66	arg1	Diabetes					70:77	Type 1 and Type 2 Diabetes	52:77	Diabetes	70:77	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.					
31687407	0	98	from	Effects	11:17	arg1	Diabetes					70:77	Type 1 and Type 2 Diabetes	52:77	Diabetes	70:77	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.					
31687407	0	98	from	Effects	11:17	arg1	Type					52:55	Type 1 and Type 2 Diabetes	52:77	Type	52:55	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.					
31687407	6	99	theme	insulin	1126:1132	arg1	expressions					1134:1144	the insulin expressions	1122:1144	the insulin expressions	1122:1144	After 28 days of treatment, plasma biochemical indicators including glycated serum proteins, total cholesterol, and triglycerides were measured and immunohistochemistry staining of the rat pancreas was performed, to study the insulin expressions.					
31687407	1	100	theme	type	303:306	arg1	diabetes					310:317	type 2 diabetes	303:317	type 2 diabetes	303:317	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	13	101	theme	experimental	2038:2049	arg1	basis					2051:2055	experimental basis	2038:2055	experimental basis for further development of KI composition	2038:2097	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	13	101	theme	experimental	2038:2049	arg1	supplement					2109:2118	a food supplement	2102:2118	a food supplement for diabetic or diabetic high-risk population	2102:2164	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	12	102	theme	extract	1891:1897	arg1	ingredient					1860:1869	single ingredient	1853:1869	single ingredient of konjac or inulin extract	1853:1897	Such effects from KI composition were better than single ingredient of konjac or inulin extract.					
31687407	2	103	theme	medium	550:555	arg1	group					562:566	medium dose group	550:566	medium dose group (KI-M group)	550:579	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	103	theme	medium	550:555	arg1	group					574:578	KI-M group	569:578	KI-M group	569:578	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	12	104	theme	konjac	1874:1879	arg1	ingredient					1860:1869	single ingredient	1853:1869	single ingredient of konjac or inulin extract	1853:1897	Such effects from KI composition were better than single ingredient of konjac or inulin extract.					
31687407	11	105	theme	RESULTS	1556:1562	arg1	use					1570:1572	RESULTS Daily use	1556:1572	RESULTS Daily use of the KI composition	1556:1594	RESULTS Daily use of the KI composition significantly decreased levels of blood glucose and blood triglycerides, as well as improved the insulin production in islets or reduced development of obesity in STZ-induced diabetic rats or in db/db mice.					
31687407	5	106	theme	glucose	862:868	arg1	test					880:883	a glucose tolerance test	860:883	a glucose tolerance test	860:883	After continuous treatment of each diet for 24 days, a glucose tolerance test was performed.					
31687407	2	107	from	group	645:649	arg1	combination					585:595	KI combination	582:595	KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group)	582:705	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	107	from	group	645:649	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	0	108	theme	Konjac	22:27	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes	0:77	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.					
31687407	7	109	theme	Type	1147:1150	arg1	diabetes					1154:1161	Type 2 diabetes	1147:1161	Type 2 diabetes	1147:1161	Type 2 diabetes was developed in db/db mice.					
31687407	8	110	theme	composition	1279:1289	arg1	groups					1237:1242	4 groups	1235:1242	4 groups: vehicle control (db/db group)	1235:1273	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	110	theme	composition	1279:1289	arg1	group					1291:1295	KI composition group	1276:1295	KI composition group (KI group)	1276:1306	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	110	theme	composition	1279:1289	arg1	group					1301:1305	KI group	1298:1305	KI group	1298:1305	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	10	111	theme	blood	1507:1511	arg1	triglycerides					1513:1525	blood triglycerides	1507:1525	blood triglycerides	1507:1525	Levels of blood glucose, body weight, and blood triglycerides were monitored at each week.					
31687407	0	112	theme	Inulin	33:38	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes	0:77	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.					
31687407	4	113	theme	blood	764:768	arg1	glucose					770:776	blood glucose	764:776	blood glucose	764:776	Levels of blood glucose were monitored at each week.					
31687407	2	114	theme	rats	380:383	arg1	total					350:354	A total	348:354	A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ)	348:415	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	12	115	theme	KI	1821:1822	arg1	composition					1824:1834	KI composition	1821:1834	KI composition	1821:1834	Such effects from KI composition were better than single ingredient of konjac or inulin extract.					
31687407	12	116	from	composition	1824:1834	arg1	effects					1808:1814	Such effects	1803:1814	Such effects from KI composition	1803:1834	Such effects from KI composition were better than single ingredient of konjac or inulin extract.					
31687407	1	117	theme	type	283:286	arg1	diabetes					290:297	type 1 diabetes	283:297	type 1 diabetes	283:297	OBJECTIVE The present study was designed to determine whether konjac and inulin extracts or their combination, konjac-inulin (KI) composition, as diet supplementary, can exert beneficial effects against type 1 diabetes and type 2 diabetes using animal models.					
31687407	0	118	dep	Konjac	22:27	arg1	Extracts					40:47	Extracts	40:47	Extracts	40:47	Protective Effects of Konjac and Inulin Extracts on Type 1 and Type 2 Diabetes.					
31687407	2	119	theme	konjac	630:635	arg1	group					645:649	konjac extract group	630:649	konjac extract group (konjac group)	630:664	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	119	theme	konjac	630:635	arg1	group					659:663	konjac group	652:663	konjac group	652:663	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	120	theme	inulin	671:676	arg1	group					686:690	inulin extract group	671:690	inulin extract group	671:690	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	121	from	group	610:614	arg1	combination					585:595	KI combination	582:595	KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group)	582:705	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	121	from	group	610:614	arg1	control					464:470	vehicle control	456:470	vehicle control (STZ group)	456:482	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	122	theme	inulin	1350:1355	arg1	group					1365:1369	inulin extract group	1350:1369	inulin extract group (inulin group)	1350:1384	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	122	theme	inulin	1350:1355	arg1	group					1379:1383	inulin group	1372:1383	inulin group	1372:1383	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	122	theme	inulin	1350:1355	arg1	groups					1237:1242	4 groups	1235:1242	4 groups: vehicle control (db/db group)	1235:1273	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	13	123	theme	protective	1984:1993	arg1	role					1995:1998	a protective role	1982:1998	a protective role	1982:1998	CONCLUSION The results of this study suggest that daily use of KI composition has a protective role on type 1 and 2 diabetes and provided experimental basis for further development of KI composition as a food supplement for diabetic or diabetic high-risk population.					
31687407	8	124	theme	extract	1316:1322	arg1	groups					1237:1242	4 groups	1235:1242	4 groups: vehicle control (db/db group)	1235:1273	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	124	theme	extract	1316:1322	arg1	group					1324:1328	konjac extract group	1309:1328	konjac extract group (konjac group)	1309:1343	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	8	124	theme	extract	1316:1322	arg1	group					1338:1342	konjac group	1331:1342	konjac group	1331:1342	A total of 28 db/db mice were divided into 4 groups: vehicle control (db/db group), KI composition group (KI group), konjac extract group (konjac group), and inulin extract group (inulin group).					
31687407	2	125	dep	METHODS	340:346	arg1	assigned					431:438	assigned	431:438	were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group)	417:705	METHODS A total of 60 diabetic (type 1) rats induced by streptozotocin (STZ) were randomly assigned to five groups: vehicle control (STZ group), KI combination at low dose group (KI-L group), KI combination at medium dose group (KI-M group), KI combination at high dose group (KI-H group), konjac extract group (konjac group), and inulin extract group (inulin group).					
30051560	0	0	theme	bacterial	79:87	arg1	communities					89:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	Structure and function of high Arctic pelagic, particle-associated and benthic bacterial communities.					
30051560	5	1	theme	ice-covered	839:849	arg1	regions					851:857	ice-covered regions	839:857	ice-covered regions	839:857	Peptide hydrolysis rates were highest in surface waters, especially at ice-free and first year ice-covered regions, and decreased with depth.					
30051560	2	2	theme	central	373:379	arg1	Ocean					388:392	the central Arctic Ocean	369:392	the central Arctic Ocean	369:392	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	5	3	dep	ice-free	815:822	arg1	regions					851:857	ice-covered regions	839:857	ice-covered regions	839:857	Peptide hydrolysis rates were highest in surface waters, especially at ice-free and first year ice-covered regions, and decreased with depth.					
30051560	4	4	theme	community	632:640	arg1	differences					656:666	community compositional differences	632:666	community compositional differences	632:666	In addition to community compositional differences, regional and depth-related patterns in enzymatic activities were observed.					
30051560	0	5	theme	benthic	71:77	arg1	communities					89:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	Structure and function of high Arctic pelagic, particle-associated and benthic bacterial communities.					
30051560	6	6	theme	wider	993:997	arg1	spectrum					999:1006	a wider spectrum	991:1006	a wider spectrum of polysaccharide hydrolase activities	991:1045	While the range of hydrolysed polysaccharides showed varying geographic patterns, particles often showed a wider spectrum of polysaccharide hydrolase activities.					
30051560	9	7	from	shifts	1556:1561	arg1	response					1568:1575	response	1568:1575	response to the changing Arctic	1568:1598	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	7	8	theme	peptidase	1063:1071	arg1	rates					1073:1077	Summed benthic peptidase rates	1048:1077	Summed benthic peptidase rates	1048:1077	Summed benthic peptidase rates differed across stations but showed similar proportions of individual enzyme activities.					
30051560	10	9	dep	Society	1700:1706	arg1	Sons					1750:1753	Sons	1750:1753	Sons	1750:1753	© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.					
30051560	9	10	theme	changing	1584:1591	arg1	Arctic					1593:1598	the changing Arctic	1580:1598	the changing Arctic	1580:1598	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	8	11	theme	direct	1301:1306	arg1	link					1308:1311	no direct link	1298:1311	no direct link	1298:1311	Analysing for potential linkages between structure and function after subtracting the effect of environmental conditions revealed no direct link, indicating functional redundancy to carry out peptide hydrolysis among pelagic microbes.					
30051560	9	12	contain	have	1606:1609	arg2	consequences					1651:1662	complex and less predictable functional consequences	1611:1662	complex and less predictable functional consequences	1611:1662	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	9	12	contain	have	1606:1609	arg1	redundancy					1517:1526	bacterial functional redundancy	1496:1526	bacterial functional redundancy	1496:1526	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	3	13	theme	community	557:565	arg1	composition					567:577	community composition	557:577	community composition	557:577	Our findings showed influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts.					
30051560	2	14	theme	pelagic	303:309	arg1	communities					354:364	pelagic, particle-associated and benthic bacterial communities	303:364	pelagic, particle-associated and benthic bacterial communities	303:364	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	0	15	theme	communities	89:99	arg1	function					14:21	function	14:21	function	14:21	Structure and function of high Arctic pelagic, particle-associated and benthic bacterial communities.					
30051560	0	15	theme	communities	89:99	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and function of high Arctic pelagic, particle-associated and benthic bacterial communities.					
30051560	5	16	theme	Peptide	744:750	arg1	rates					763:767	Peptide hydrolysis rates	744:767	Peptide hydrolysis rates	744:767	Peptide hydrolysis rates were highest in surface waters, especially at ice-free and first year ice-covered regions, and decreased with depth.					
30051560	7	17	theme	Summed	1048:1053	arg1	rates					1073:1077	Summed benthic peptidase rates	1048:1077	Summed benthic peptidase rates	1048:1077	Summed benthic peptidase rates differed across stations but showed similar proportions of individual enzyme activities.					
30051560	3	18	theme	association	542:552	arg1	influences					491:500	influences	491:500	influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts	491:614	Our findings showed influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts.					
30051560	3	19	theme	conditions	518:527	arg1	influences					491:500	influences	491:500	influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts	491:614	Our findings showed influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts.					
30051560	7	20	theme	benthic	1055:1061	arg1	rates					1073:1077	Summed benthic peptidase rates	1048:1077	Summed benthic peptidase rates	1048:1077	Summed benthic peptidase rates differed across stations but showed similar proportions of individual enzyme activities.					
30051560	2	21	theme	enzymatic	279:287	arg1	activities					289:298	enzymatic activities	279:298	enzymatic activities	279:298	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	9	22	theme	complex	1611:1617	arg1	consequences					1651:1662	complex and less predictable functional consequences	1611:1662	complex and less predictable functional consequences	1611:1662	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	8	23	theme	pelagic	1385:1391	arg1	microbes					1393:1400	pelagic microbes	1385:1400	pelagic microbes	1385:1400	Analysing for potential linkages between structure and function after subtracting the effect of environmental conditions revealed no direct link, indicating functional redundancy to carry out peptide hydrolysis among pelagic microbes.					
30051560	4	24	theme	compositional	642:654	arg1	differences					656:666	community compositional differences	632:666	community compositional differences	632:666	In addition to community compositional differences, regional and depth-related patterns in enzymatic activities were observed.					
30051560	9	25	theme	environmental	1470:1482	arg1	conditions					1484:1493	environmental conditions	1470:1493	environmental conditions	1470:1493	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	9	26	theme	compositional	1542:1554	arg1	shifts					1556:1561	compositional shifts	1542:1561	compositional shifts - in response to the changing Arctic	1542:1598	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	8	27	theme	functional	1325:1334	arg1	redundancy					1336:1345	functional redundancy	1325:1345	functional redundancy to carry out peptide hydrolysis among pelagic microbes	1325:1400	Analysing for potential linkages between structure and function after subtracting the effect of environmental conditions revealed no direct link, indicating functional redundancy to carry out peptide hydrolysis among pelagic microbes.					
30051560	8	28	theme	conditions	1278:1287	arg1	effect					1254:1259	the effect	1250:1259	the effect of environmental conditions	1250:1287	Analysing for potential linkages between structure and function after subtracting the effect of environmental conditions revealed no direct link, indicating functional redundancy to carry out peptide hydrolysis among pelagic microbes.					
30051560	8	29	theme	environmental	1264:1276	arg1	conditions					1278:1287	environmental conditions	1264:1287	environmental conditions	1264:1287	Analysing for potential linkages between structure and function after subtracting the effect of environmental conditions revealed no direct link, indicating functional redundancy to carry out peptide hydrolysis among pelagic microbes.					
30051560	2	30	dep	structure	265:273	arg1	the					261:263	the	261:263	the	261:263	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	3	31	dep	pelagic-benthic	590:604	arg1	contrasts					606:614	contrasts	606:614	contrasts	606:614	Our findings showed influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts.					
30051560	8	32	theme	potential	1182:1190	arg1	linkages					1192:1199	potential linkages	1182:1199	potential linkages between structure and function	1182:1230	Analysing for potential linkages between structure and function after subtracting the effect of environmental conditions revealed no direct link, indicating functional redundancy to carry out peptide hydrolysis among pelagic microbes.					
30051560	2	33	theme	particle-associated	312:330	arg1	communities					354:364	pelagic, particle-associated and benthic bacterial communities	303:364	pelagic, particle-associated and benthic bacterial communities	303:364	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	8	34	theme	peptide	1360:1366	arg1	hydrolysis					1368:1377	peptide hydrolysis	1360:1377	peptide hydrolysis	1360:1377	Analysing for potential linkages between structure and function after subtracting the effect of environmental conditions revealed no direct link, indicating functional redundancy to carry out peptide hydrolysis among pelagic microbes.					
30051560	9	35	theme	bacterial	1496:1504	arg1	redundancy					1517:1526	bacterial functional redundancy	1496:1526	bacterial functional redundancy	1496:1526	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	1	36	theme	Arctic	102:107	arg1	microbes					116:123	Arctic marine microbes	102:123	Arctic marine microbes	102:123	Arctic marine microbes are affected by environmental changes that may ultimately influence their functions in carbon cycling.					
30051560	2	37	theme	microbial	427:435	arg1	relationships					456:468	microbial structure-function relationships	427:468	microbial structure-function relationships	427:468	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	2	38	theme	structure-function	437:454	arg1	relationships					456:468	microbial structure-function relationships	427:468	microbial structure-function relationships	427:468	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	9	39	theme	functional	1506:1515	arg1	redundancy					1517:1526	bacterial functional redundancy	1496:1526	bacterial functional redundancy	1496:1526	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	5	40	theme	surface	785:791	arg1	waters					793:798	surface waters	785:798	surface waters	785:798	Peptide hydrolysis rates were highest in surface waters, especially at ice-free and first year ice-covered regions, and decreased with depth.					
30051560	4	41	dep	differences	656:666	arg1	addition					620:627	addition	620:627	addition	620:627	In addition to community compositional differences, regional and depth-related patterns in enzymatic activities were observed.					
30051560	1	42	theme	marine	109:114	arg1	microbes					116:123	Arctic marine microbes	102:123	Arctic marine microbes	102:123	Arctic marine microbes are affected by environmental changes that may ultimately influence their functions in carbon cycling.					
30051560	2	43	from	structure	265:273	arg1	Ocean					388:392	the central Arctic Ocean	369:392	the central Arctic Ocean	369:392	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	1	44	theme	carbon	212:217	arg1	cycling					219:225	carbon cycling	212:225	carbon cycling	212:225	Arctic marine microbes are affected by environmental changes that may ultimately influence their functions in carbon cycling.					
30051560	0	45	theme	Arctic	31:36	arg1	communities					89:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	Structure and function of high Arctic pelagic, particle-associated and benthic bacterial communities.					
30051560	3	46	theme	particle	533:540	arg1	association					542:552	particle association	533:552	particle association	533:552	Our findings showed influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts.					
30051560	6	47	theme	geographic	947:956	arg1	patterns					958:965	varying geographic patterns	939:965	varying geographic patterns	939:965	While the range of hydrolysed polysaccharides showed varying geographic patterns, particles often showed a wider spectrum of polysaccharide hydrolase activities.					
30051560	4	48	from	patterns	696:703	arg1	activities					718:727	enzymatic activities	708:727	enzymatic activities	708:727	In addition to community compositional differences, regional and depth-related patterns in enzymatic activities were observed.					
30051560	9	49	theme	functional	1640:1649	arg1	consequences					1651:1662	complex and less predictable functional consequences	1611:1662	complex and less predictable functional consequences	1611:1662	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	0	50	theme	high	26:29	arg1	communities					89:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	Structure and function of high Arctic pelagic, particle-associated and benthic bacterial communities.					
30051560	9	51	theme	predictable	1628:1638	arg1	consequences					1651:1662	complex and less predictable functional consequences	1611:1662	complex and less predictable functional consequences	1611:1662	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	6	52	theme	varying	939:945	arg1	patterns					958:965	varying geographic patterns	939:965	varying geographic patterns	939:965	While the range of hydrolysed polysaccharides showed varying geographic patterns, particles often showed a wider spectrum of polysaccharide hydrolase activities.					
30051560	4	53	theme	enzymatic	708:716	arg1	activities					718:727	enzymatic activities	708:727	enzymatic activities	708:727	In addition to community compositional differences, regional and depth-related patterns in enzymatic activities were observed.					
30051560	6	54	theme	activities	1036:1045	arg1	spectrum					999:1006	a wider spectrum	991:1006	a wider spectrum of polysaccharide hydrolase activities	991:1045	While the range of hydrolysed polysaccharides showed varying geographic patterns, particles often showed a wider spectrum of polysaccharide hydrolase activities.					
30051560	5	55	theme	hydrolysis	752:761	arg1	rates					763:767	Peptide hydrolysis rates	744:767	Peptide hydrolysis rates	744:767	Peptide hydrolysis rates were highest in surface waters, especially at ice-free and first year ice-covered regions, and decreased with depth.					
30051560	10	56	theme	Applied	1712:1718	arg1	Microbiology					1720:1731	Applied Microbiology	1712:1731	Applied Microbiology	1712:1731	© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.					
30051560	0	57	theme	pelagic	38:44	arg1	communities					89:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	Structure and function of high Arctic pelagic, particle-associated and benthic bacterial communities.					
30051560	6	58	theme	hydrolase	1026:1034	arg1	activities					1036:1045	polysaccharide hydrolase activities	1011:1045	polysaccharide hydrolase activities	1011:1045	While the range of hydrolysed polysaccharides showed varying geographic patterns, particles often showed a wider spectrum of polysaccharide hydrolase activities.					
30051560	6	59	theme	polysaccharides	916:930	arg1	range					896:900	the range	892:900	the range of hydrolysed polysaccharides	892:930	While the range of hydrolysed polysaccharides showed varying geographic patterns, particles often showed a wider spectrum of polysaccharide hydrolase activities.					
30051560	3	60	from	influences	491:500	arg1	pelagic-benthic					590:604	pelagic-benthic	590:604	pelagic-benthic	590:604	Our findings showed influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts.					
30051560	3	60	from	influences	491:500	arg1	composition					567:577	community composition	557:577	community composition	557:577	Our findings showed influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts.					
30051560	2	61	theme	communities	354:364	arg1	activities					289:298	enzymatic activities	279:298	enzymatic activities	279:298	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	2	61	theme	communities	354:364	arg1	structure					265:273	structure	265:273	structure	265:273	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	6	62	theme	polysaccharide	1011:1024	arg1	activities					1036:1045	polysaccharide hydrolase activities	1011:1045	polysaccharide hydrolase activities	1011:1045	While the range of hydrolysed polysaccharides showed varying geographic patterns, particles often showed a wider spectrum of polysaccharide hydrolase activities.					
30051560	7	63	theme	individual	1138:1147	arg1	activities					1156:1165	individual enzyme activities	1138:1165	individual enzyme activities	1138:1165	Summed benthic peptidase rates differed across stations but showed similar proportions of individual enzyme activities.					
30051560	6	64	theme	hydrolysed	905:914	arg1	polysaccharides					916:930	hydrolysed polysaccharides	905:930	hydrolysed polysaccharides	905:930	While the range of hydrolysed polysaccharides showed varying geographic patterns, particles often showed a wider spectrum of polysaccharide hydrolase activities.					
30051560	7	65	theme	similar	1115:1121	arg1	proportions					1123:1133	similar proportions	1115:1133	similar proportions of individual enzyme activities	1115:1165	Summed benthic peptidase rates differed across stations but showed similar proportions of individual enzyme activities.					
30051560	2	66	theme	bacterial	344:352	arg1	communities					354:364	pelagic, particle-associated and benthic bacterial communities	303:364	pelagic, particle-associated and benthic bacterial communities	303:364	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	4	67	theme	regional	669:676	arg1	patterns					696:703	regional and depth-related patterns	669:703	regional and depth-related patterns in enzymatic activities	669:727	In addition to community compositional differences, regional and depth-related patterns in enzymatic activities were observed.					
30051560	7	68	theme	enzyme	1149:1154	arg1	activities					1156:1165	individual enzyme activities	1138:1165	individual enzyme activities	1138:1165	Summed benthic peptidase rates differed across stations but showed similar proportions of individual enzyme activities.					
30051560	2	69	theme	benthic	336:342	arg1	communities					354:364	pelagic, particle-associated and benthic bacterial communities	303:364	pelagic, particle-associated and benthic bacterial communities	303:364	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	3	70	theme	hydrographic	505:516	arg1	conditions					518:527	hydrographic conditions	505:527	hydrographic conditions	505:527	Our findings showed influences of hydrographic conditions and particle association on community composition, and sharp pelagic-benthic contrasts.					
30051560	5	71	theme	first	828:832	arg1	year					834:837	first year	828:837	first year	828:837	Peptide hydrolysis rates were highest in surface waters, especially at ice-free and first year ice-covered regions, and decreased with depth.					
30051560	9	72	theme	community	1415:1423	arg1	composition					1425:1435	community composition	1415:1435	community composition	1415:1435	Thus, while community composition and activities are influenced by environmental conditions, bacterial functional redundancy suggests that compositional shifts - in response to the changing Arctic - may have complex and less predictable functional consequences than previously anticipated.					
30051560	7	73	theme	activities	1156:1165	arg1	proportions					1123:1133	similar proportions	1115:1133	similar proportions of individual enzyme activities	1115:1165	Summed benthic peptidase rates differed across stations but showed similar proportions of individual enzyme activities.					
30051560	1	74	theme	environmental	141:153	arg1	changes					155:161	environmental changes	141:161	environmental changes that may ultimately influence their functions in carbon cycling	141:225	Arctic marine microbes are affected by environmental changes that may ultimately influence their functions in carbon cycling.					
30051560	2	75	from	activities	289:298	arg1	Ocean					388:392	the central Arctic Ocean	369:392	the central Arctic Ocean	369:392	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	4	76	theme	depth-related	682:694	arg1	patterns					696:703	regional and depth-related patterns	669:703	regional and depth-related patterns in enzymatic activities	669:727	In addition to community compositional differences, regional and depth-related patterns in enzymatic activities were observed.					
30051560	0	77	theme	particle-associated	47:65	arg1	communities					89:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	high Arctic pelagic, particle-associated and benthic bacterial communities	26:99	Structure and function of high Arctic pelagic, particle-associated and benthic bacterial communities.					
30051560	2	78	used	used	399:402	arg2	we					234:235	we	234:235	we	234:235	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
30051560	2	79	theme	Arctic	381:386	arg1	Ocean					388:392	the central Arctic Ocean	369:392	the central Arctic Ocean	369:392	Here, we investigated in concert the structure and enzymatic activities of pelagic, particle-associated and benthic bacterial communities in the central Arctic Ocean, and used these data to evaluate microbial structure-function relationships.					
29299925	0	0	theme	Nanosheet-Supported	78:96	arg1	Recognitions					109:120	MoS2 Nanosheet-Supported Host-Guest Recognitions	73:120	MoS2 Nanosheet-Supported Host-Guest Recognitions	73:120	A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring Based on MoS2 Nanosheet-Supported Host-Guest Recognitions.					
29299925	1	1	theme	antibacterial	339:351	arg1	mechanisms					353:362	antibacterial mechanisms	339:362	antibacterial mechanisms	339:362	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	6	2	theme	subtle	1115:1120	arg1	change					1122:1127	the subtle change	1111:1127	tracking the subtle change of amino acid composition of PG	1102:1159	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	1	3	theme	wall	250:253	arg1	biosynthesis					255:266	cell wall biosynthesis	245:266	cell wall biosynthesis	245:266	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	0	4	theme	MoS2	73:76	arg1	Recognitions					109:120	MoS2 Nanosheet-Supported Host-Guest Recognitions	73:120	MoS2 Nanosheet-Supported Host-Guest Recognitions	73:120	A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring Based on MoS2 Nanosheet-Supported Host-Guest Recognitions.					
29299925	5	5	theme	indicator	769:777	arg1	strategy					792:799	indicator displacement strategy	769:799	indicator displacement strategy	769:799	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	2	6	theme	l-amino	494:500	arg1	acids					502:506	d- and l-amino acids	487:506	d- and l-amino acids	487:506	However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG.					
29299925	6	7	theme	composition	1143:1153	arg1	change					1122:1127	the subtle change	1111:1127	tracking the subtle change of amino acid composition of PG	1102:1159	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	6	8	used	used	1059:1062	arg2	array					1039:1043	The sensor array	1028:1043	The sensor array	1028:1043	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	3	9	theme	chiral	532:537	arg1	array					546:550	a chiral sensor array	530:550	a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition	530:619	Here we report a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition.					
29299925	0	10	theme	Host-Guest	98:107	arg1	Recognitions					109:120	MoS2 Nanosheet-Supported Host-Guest Recognitions	73:120	MoS2 Nanosheet-Supported Host-Guest Recognitions	73:120	A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring Based on MoS2 Nanosheet-Supported Host-Guest Recognitions.					
29299925	2	11	from	coexistence	472:482	arg1	PG					511:512	PG	511:512	PG	511:512	However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG.					
29299925	3	12	theme	sensor	539:544	arg1	array					546:550	a chiral sensor array	530:550	a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition	530:619	Here we report a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition.					
29299925	6	13	theme	amino	1132:1136	arg1	composition					1143:1153	amino acid composition	1132:1153	amino acid composition of PG	1132:1159	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	4	14	theme	accurate	726:733	arg1	sensing					748:754	more accurate and specific sensing	721:754	more accurate and specific sensing	721:754	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	5	15	theme	corresponding	981:993	arg1	d-enantiomers					998:1010	the corresponding 19 d-enantiomers	977:1010	the corresponding 19 d-enantiomers	977:1010	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	5	16	theme	discriminant	888:899	arg1	LDA					911:913	LDA	911:913	LDA	911:913	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	5	16	theme	discriminant	888:899	arg1	analysis					901:908	linear discriminant analysis	881:908	linear discriminant analysis (LDA)	881:914	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	4	17	theme	nanosheets	664:673	arg1	units					704:708	receptor units	695:708	receptor units	695:708	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	4	17	theme	nanosheets	664:673	arg1	Multitypes					622:631	Multitypes	622:631	Multitypes of host molecule modified MoS2 nanosheets (MNSs)	622:680	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	2	18	theme	d-	487:488	arg1	acids					502:506	d- and l-amino acids	487:506	d- and l-amino acids	487:506	However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG.					
29299925	1	19	from	change	146:151	arg1	PG					234:235	PG	234:235	PG	234:235	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	1	19	from	change	146:151	arg1	peptidoglycan					219:231	bacterial peptidoglycan	209:231	bacterial peptidoglycan (PG)	209:236	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	5	20	theme	distinct	806:813	arg1	patterns					854:861	the distinct and reproducible fluorescence-response patterns	802:861	the distinct and reproducible fluorescence-response patterns	802:861	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	2	21	theme	reported	402:409	arg1	methods					411:417	reported methods	402:417	reported methods	402:417	However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG.					
29299925	0	22	theme	Chiral	2:7	arg1	Array					16:20	A Chiral Sensor Array	0:20	A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring	0:62	A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring Based on MoS2 Nanosheet-Supported Host-Guest Recognitions.					
29299925	3	23	theme	PG	556:557	arg1	biosynthesis					559:570	PG biosynthesis	556:570	PG biosynthesis monitoring through chiral amino acid recognition	556:619	Here we report a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition.					
29299925	6	24	from	biosynthesis	1200:1211	arg1	status					1233:1238	different growth status	1216:1238	different growth status	1216:1238	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	6	24	from	biosynthesis	1200:1211	arg1	stimulation					1267:1277	exogenous d-amino acid stimulation	1244:1277	exogenous d-amino acid stimulation	1244:1277	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	1	25	theme	amino	194:198	arg1	acids					200:204	amino acids	194:204	amino acids	194:204	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	2	26	theme	analysis	454:461	arg1	ability					436:442	the ability	432:442	the ability of chiral analysis with the coexistence of d- and l-amino acids in PG	432:512	However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG.					
29299925	6	27	theme	growth	1226:1231	arg1	status					1233:1238	different growth status	1216:1238	different growth status	1216:1238	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	5	28	theme	linear	881:886	arg1	LDA					911:913	LDA	911:913	LDA	911:913	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	5	28	theme	linear	881:886	arg1	analysis					901:908	linear discriminant analysis	881:908	linear discriminant analysis (LDA)	881:914	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	1	29	theme	acids	200:204	arg1	species					183:189	species	183:189	species	183:189	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	1	29	theme	acids	200:204	arg1	chirality					169:177	chirality	169:177	chirality	169:177	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	2	30	theme	chiral	447:452	arg1	analysis					454:461	chiral analysis	447:461	chiral analysis with the coexistence of d- and l-amino acids in PG	447:512	However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG.					
29299925	1	31	theme	bacterial	287:295	arg1	taxonomy					297:304	bacterial taxonomy	287:304	bacterial taxonomy	287:304	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	4	32	theme	specific	739:746	arg1	sensing					748:754	more accurate and specific sensing	721:754	more accurate and specific sensing	721:754	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	4	33	theme	host	636:639	arg1	nanosheets					664:673	host molecule modified MoS2 nanosheets	636:673	host molecule modified MoS2 nanosheets (MNSs)	636:680	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	4	33	theme	host	636:639	arg1	MNSs					676:679	MNSs	676:679	MNSs	676:679	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	0	34	theme	Sensor	9:14	arg1	Array					16:20	A Chiral Sensor Array	0:20	A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring	0:62	A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring Based on MoS2 Nanosheet-Supported Host-Guest Recognitions.					
29299925	6	35	theme	sensor	1032:1037	arg1	array					1039:1043	The sensor array	1028:1043	The sensor array	1028:1043	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	6	36	theme	PG	1158:1159	arg1	composition					1143:1153	amino acid composition	1132:1153	amino acid composition of PG	1132:1159	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	1	37	theme	bacterial	209:217	arg1	PG					234:235	PG	234:235	PG	234:235	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	1	37	theme	bacterial	209:217	arg1	peptidoglycan					219:231	bacterial peptidoglycan	209:231	bacterial peptidoglycan (PG)	209:236	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	0	38	theme	Peptidoglycan	26:38	arg1	Monitoring					53:62	Peptidoglycan Biosynthesis Monitoring	26:62	Peptidoglycan Biosynthesis Monitoring	26:62	A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring Based on MoS2 Nanosheet-Supported Host-Guest Recognitions.					
29299925	4	39	theme	receptor	695:702	arg1	units					704:708	receptor units	695:708	receptor units	695:708	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	4	39	theme	receptor	695:702	arg1	Multitypes					622:631	Multitypes	622:631	Multitypes of host molecule modified MoS2 nanosheets (MNSs)	622:680	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	3	40	theme	chiral	591:596	arg1	recognition					609:619	chiral amino acid recognition	591:619	chiral amino acid recognition	591:619	Here we report a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition.					
29299925	6	41	theme	bacterial	1080:1088	arg1	species					1090:1096	bacterial species	1080:1096	bacterial species	1080:1096	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	4	42	theme	modified	650:657	arg1	nanosheets					664:673	host molecule modified MoS2 nanosheets	636:673	host molecule modified MoS2 nanosheets (MNSs)	636:680	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	4	42	theme	modified	650:657	arg1	MNSs					676:679	MNSs	676:679	MNSs	676:679	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	5	43	theme	displacement	779:790	arg1	strategy					792:799	indicator displacement strategy	769:799	indicator displacement strategy	769:799	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	3	44	theme	amino	598:602	arg1	recognition					609:619	chiral amino acid recognition	591:619	chiral amino acid recognition	591:619	Here we report a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition.					
29299925	4	45	theme	molecule	641:648	arg1	nanosheets					664:673	host molecule modified MoS2 nanosheets	636:673	host molecule modified MoS2 nanosheets (MNSs)	636:680	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	4	45	theme	molecule	641:648	arg1	MNSs					676:679	MNSs	676:679	MNSs	676:679	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	6	46	dep	tracking	1102:1109	arg1	change					1122:1127	the subtle change	1111:1127	tracking the subtle change of amino acid composition of PG	1102:1159	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	6	47	theme	d-amino	1254:1260	arg1	acid					1262:1265	exogenous d-amino acid	1244:1265	exogenous d-amino acid stimulation	1244:1277	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	0	48	theme	Biosynthesis	40:51	arg1	Monitoring					53:62	Peptidoglycan Biosynthesis Monitoring	26:62	Peptidoglycan Biosynthesis Monitoring	26:62	A Chiral Sensor Array for Peptidoglycan Biosynthesis Monitoring Based on MoS2 Nanosheet-Supported Host-Guest Recognitions.					
29299925	6	49	theme	acid	1262:1265	arg1	stimulation					1267:1277	exogenous d-amino acid stimulation	1244:1277	exogenous d-amino acid stimulation	1244:1277	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	6	50	theme	exogenous	1244:1252	arg1	acid					1262:1265	exogenous d-amino acid	1244:1265	exogenous d-amino acid stimulation	1244:1277	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	1	51	theme	dynamic	138:144	arg1	change					146:151	the dynamic change	134:151	the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG)	134:236	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
29299925	6	52	theme	different	1216:1224	arg1	status					1233:1238	different growth status	1216:1238	different growth status	1216:1238	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	4	53	used	used	687:690	arg2	Multitypes					622:631	Multitypes	622:631	Multitypes of host molecule modified MoS2 nanosheets (MNSs)	622:680	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	4	53	used	used	687:690	arg2	units					704:708	receptor units	695:708	receptor units	695:708	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	2	54	with	analysis	454:461	arg1	coexistence					472:482	the coexistence	468:482	the coexistence of d- and l-amino acids in PG	468:512	However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG.					
29299925	5	55	theme	achiral	943:949	arg1	acids					967:971	Gly, 19 l-amino acids	951:971	acids	967:971	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	5	56	theme	l-amino	959:965	arg1	acids					967:971	Gly, 19 l-amino acids	951:971	acids	967:971	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	5	57	theme	Gly	951:953	arg1	acids					967:971	Gly, 19 l-amino acids	951:971	acids	967:971	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	6	58	theme	acid	1138:1141	arg1	composition					1143:1153	amino acid composition	1132:1153	amino acid composition of PG	1132:1159	The sensor array has also been used for identifying bacterial species and tracking the subtle change of amino acid composition of PG including chirality and species during biosynthesis in different growth status and exogenous d-amino acid stimulation.					
29299925	3	59	theme	acid	604:607	arg1	recognition					609:619	chiral amino acid recognition	591:619	chiral amino acid recognition	591:619	Here we report a chiral sensor array for PG biosynthesis monitoring through chiral amino acid recognition.					
29299925	5	60	theme	fluorescence-response	832:852	arg1	patterns					854:861	the distinct and reproducible fluorescence-response patterns	802:861	the distinct and reproducible fluorescence-response patterns	802:861	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	4	61	theme	MoS2	659:662	arg1	nanosheets					664:673	host molecule modified MoS2 nanosheets	636:673	host molecule modified MoS2 nanosheets (MNSs)	636:680	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	4	61	theme	MoS2	659:662	arg1	MNSs					676:679	MNSs	676:679	MNSs	676:679	Multitypes of host molecule modified MoS2 nanosheets (MNSs) were used as receptor units to achieve more accurate and specific sensing.					
29299925	2	62	theme	acids	502:506	arg1	coexistence					472:482	the coexistence	468:482	the coexistence of d- and l-amino acids in PG	468:512	However, this is challenging because reported methods usually lack the ability of chiral analysis with the coexistence of d- and l-amino acids in PG.					
29299925	5	63	theme	reproducible	819:830	arg1	patterns					854:861	the distinct and reproducible fluorescence-response patterns	802:861	the distinct and reproducible fluorescence-response patterns	802:861	By applying indicator displacement strategy, the distinct and reproducible fluorescence-response patterns were obtained for linear discriminant analysis (LDA) to accurately discriminate achiral Gly, 19 l-amino acids and the corresponding 19 d-enantiomers simultaneously.					
29299925	1	64	theme	cell	245:248	arg1	biosynthesis					255:266	cell wall biosynthesis	245:266	cell wall biosynthesis	245:266	Monitoring the dynamic change with respect to chirality and species of amino acids in bacterial peptidoglycan (PG) during cell wall biosynthesis is correlated with bacterial taxonomy, physiology, micropathology, and antibacterial mechanisms.					
31173831	5	0	theme	distal	1165:1170	arg1	intestine					1178:1186	the distal small intestine	1161:1186	the distal small intestine	1161:1186	2B-HBEO-G minimized exposure of the loaded HBEO in the simulated gastric fluids and delivered the oil to the distal small intestine.					
31173831	1	1	from	use	200:202	arg1	food					225:228	food	225:228	food	225:228	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	3	2	theme	coated	833:838	arg1	microcapsules					840:852	the 3, 1, and 2% beeswax-CMC coated microcapsules	804:852	the 3, 1, and 2% beeswax-CMC coated microcapsules	804:852	After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.					
31173831	1	3	theme	health	260:265	arg1	benefits					267:274	health benefits	260:274	health benefits	260:274	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	0	4	theme	intestinal	89:98	arg1	delivery					100:107	intestinal delivery	89:107	intestinal delivery	89:107	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	2	5	from	effects	474:480	arg1	cellulose					556:564	carboxymethyl cellulose	542:564	carboxymethyl cellulose	542:564	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	5	from	effects	474:480	arg1	beeswax					515:521	beeswax	515:521	beeswax (1, 2, and 3%)	515:536	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	5	from	effects	474:480	arg1	CMC					567:569	CMC	567:569	CMC	567:569	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	5	from	effects	474:480	arg1	stability					591:599	the stability	587:599	the stability	587:599	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	5	from	effects	474:480	arg1	%					535:535	1, 2, and 3%	524:535	1, 2, and 3%	524:535	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	5	from	effects	474:480	arg1	delivery					605:612	delivery	605:612	delivery	605:612	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	1	6	from	interests	183:191	arg1	use					200:202	the use	196:202	the use of essential oils in food and feed additives to promote health benefits	196:274	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	5	7	theme	simulated	1111:1119	arg1	fluids					1129:1134	the simulated gastric fluids	1107:1134	the simulated gastric fluids	1107:1134	2B-HBEO-G minimized exposure of the loaded HBEO in the simulated gastric fluids and delivered the oil to the distal small intestine.					
31173831	2	8	from	cellulose	556:564	arg1	effects					474:480	the effects	470:480	the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation	470:710	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	8	from	cellulose	556:564	arg1	composite					500:508	emulsion-based composite	485:508	emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating	485:582	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	0	9	theme	holy	112:115	arg1	microcapsules					152:164	holy basil essential oil-loaded gelatin microcapsules	112:164	holy basil essential oil-loaded gelatin microcapsules	112:164	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	0	10	from	Effects	0:6	arg1	stability					75:83	shelf-life stability	64:83	shelf-life stability	64:83	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	0	10	from	Effects	0:6	arg1	delivery					100:107	intestinal delivery	89:107	intestinal delivery	89:107	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	2	11	theme	essential	628:636	arg1	microcapsules					657:669	holy basil essential oil-loaded gelatin microcapsules	617:669	holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation	617:710	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	11	theme	essential	628:636	arg1	HBEO-G					672:677	HBEO-G	672:677	HBEO-G	672:677	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	4	12	theme	beeswax-CMC	895:905	arg1	2B-HBEO-G					928:936	2B-HBEO-G	928:936	2B-HBEO-G	928:936	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	4	12	theme	beeswax-CMC	895:905	arg1	microcapsule					914:925	only the 2% beeswax-CMC coated microcapsule	883:925	only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G)	883:937	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	4	13	theme	antibacterial	970:982	arg1	activities					984:993	the antibacterial activities	966:993	the antibacterial activities of all microcapsules	966:1014	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	2	14	theme	basil	622:626	arg1	microcapsules					657:669	holy basil essential oil-loaded gelatin microcapsules	617:669	holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation	617:710	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	14	theme	basil	622:626	arg1	HBEO-G					672:677	HBEO-G	672:677	HBEO-G	672:677	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	1	15	theme	several	277:283	arg1	studies					285:291	several studies	277:291	several studies	277:291	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	0	16	theme	essential	123:131	arg1	microcapsules					152:164	holy basil essential oil-loaded gelatin microcapsules	112:164	holy basil essential oil-loaded gelatin microcapsules	112:164	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	3	17	theme	3-month	719:725	arg1	storage					727:733	3-month storage	719:733	3-month storage	719:733	After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.					
31173831	2	18	theme	holy	617:620	arg1	microcapsules					657:669	holy basil essential oil-loaded gelatin microcapsules	617:669	holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation	617:710	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	18	theme	holy	617:620	arg1	HBEO-G					672:677	HBEO-G	672:677	HBEO-G	672:677	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	0	19	theme	basil	117:121	arg1	microcapsules					152:164	holy basil essential oil-loaded gelatin microcapsules	112:164	holy basil essential oil-loaded gelatin microcapsules	112:164	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	5	20	from	exposure	1076:1083	arg1	fluids					1129:1134	the simulated gastric fluids	1107:1134	the simulated gastric fluids	1107:1134	2B-HBEO-G minimized exposure of the loaded HBEO in the simulated gastric fluids and delivered the oil to the distal small intestine.					
31173831	5	21	theme	small	1172:1176	arg1	intestine					1178:1186	the distal small intestine	1161:1186	the distal small intestine	1161:1186	2B-HBEO-G minimized exposure of the loaded HBEO in the simulated gastric fluids and delivered the oil to the distal small intestine.					
31173831	4	22	theme	coated	907:912	arg1	2B-HBEO-G					928:936	2B-HBEO-G	928:936	2B-HBEO-G	928:936	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	4	22	theme	coated	907:912	arg1	microcapsule					914:925	only the 2% beeswax-CMC coated microcapsule	883:925	only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G)	883:937	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	2	23	theme	composite	500:508	arg1	effects					474:480	the effects	470:480	the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation	470:710	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	5	24	from	fluids	1129:1134	arg1	exposure					1076:1083	exposure	1076:1083	exposure of the loaded HBEO in the simulated gastric fluids	1076:1134	2B-HBEO-G minimized exposure of the loaded HBEO in the simulated gastric fluids and delivered the oil to the distal small intestine.					
31173831	5	25	theme	gastric	1121:1127	arg1	fluids					1129:1134	the simulated gastric fluids	1107:1134	the simulated gastric fluids	1107:1134	2B-HBEO-G minimized exposure of the loaded HBEO in the simulated gastric fluids and delivered the oil to the distal small intestine.					
31173831	5	26	theme	loaded	1092:1097	arg1	HBEO					1099:1102	the loaded HBEO	1088:1102	the loaded HBEO in the simulated gastric fluids	1088:1134	2B-HBEO-G minimized exposure of the loaded HBEO in the simulated gastric fluids and delivered the oil to the distal small intestine.					
31173831	3	27	located	observed	772:779	arg1	HBEO-G					784:789	HBEO-G	784:789	HBEO-G	784:789	After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.					
31173831	3	27	located	observed	772:779	arg2	content					760:766	the highest surface oil content	736:766	the highest surface oil content	736:766	After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.					
31173831	2	28	theme	microcapsules	657:669	arg1	delivery					605:612	delivery	605:612	delivery	605:612	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	28	theme	microcapsules	657:669	arg1	stability					591:599	the stability	587:599	the stability	587:599	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	1	29	theme	essential	207:215	arg1	oils					217:220	essential oils	207:220	essential oils in food	207:228	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	0	30	theme	cellulose	33:41	arg1	coating					53:59	beeswax-carboxymethyl cellulose composite coating	11:59	beeswax-carboxymethyl cellulose composite coating	11:59	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	3	31	theme	highest	740:746	arg1	content					760:766	the highest surface oil content	736:766	the highest surface oil content	736:766	After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.					
31173831	0	32	theme	gelatin	144:150	arg1	microcapsules					152:164	holy basil essential oil-loaded gelatin microcapsules	112:164	holy basil essential oil-loaded gelatin microcapsules	112:164	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	2	33	theme	gelatin	649:655	arg1	microcapsules					657:669	holy basil essential oil-loaded gelatin microcapsules	617:669	holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation	617:710	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	33	theme	gelatin	649:655	arg1	HBEO-G					672:677	HBEO-G	672:677	HBEO-G	672:677	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	34	theme	oil-loaded	638:647	arg1	microcapsules					657:669	holy basil essential oil-loaded gelatin microcapsules	617:669	holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation	617:710	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	34	theme	oil-loaded	638:647	arg1	HBEO-G					672:677	HBEO-G	672:677	HBEO-G	672:677	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	1	35	theme	oils	217:220	arg1	use					200:202	the use	196:202	the use of essential oils in food and feed additives to promote health benefits	196:274	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	0	36	theme	beeswax-carboxymethyl	11:31	arg1	coating					53:59	beeswax-carboxymethyl cellulose composite coating	11:59	beeswax-carboxymethyl cellulose composite coating	11:59	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	0	37	theme	oil-loaded	133:142	arg1	microcapsules					152:164	holy basil essential oil-loaded gelatin microcapsules	112:164	holy basil essential oil-loaded gelatin microcapsules	112:164	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	3	38	dep	observed	772:779	arg1	followed					792:799	followed	792:799	followed by the 3, 1, and 2% beeswax-CMC coated microcapsules	792:852	After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.					
31173831	5	39	from	HBEO	1099:1102	arg1	fluids					1129:1134	the simulated gastric fluids	1107:1134	the simulated gastric fluids	1107:1134	2B-HBEO-G minimized exposure of the loaded HBEO in the simulated gastric fluids and delivered the oil to the distal small intestine.					
31173831	0	40	theme	coating	53:59	arg1	Effects					0:6	Effects	0:6	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.	0:165	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	2	41	from	beeswax	515:521	arg1	effects					474:480	the effects	470:480	the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation	470:710	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	2	41	from	beeswax	515:521	arg1	composite					500:508	emulsion-based composite	485:508	emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating	485:582	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	0	42	theme	composite	43:51	arg1	coating					53:59	beeswax-carboxymethyl cellulose composite coating	11:59	beeswax-carboxymethyl cellulose composite coating	11:59	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	0	43	theme	microcapsules	152:164	arg1	stability					75:83	shelf-life stability	64:83	shelf-life stability	64:83	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	0	43	theme	microcapsules	152:164	arg1	delivery					100:107	intestinal delivery	89:107	intestinal delivery	89:107	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	1	44	theme	release	420:426	arg1	profiles					428:435	their release profiles	414:435	their release profiles	414:435	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	1	45	from	food	225:228	arg1	use					200:202	the use	196:202	the use of essential oils in food and feed additives to promote health benefits	196:274	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	3	46	theme	oil	756:758	arg1	content					760:766	the highest surface oil content	736:766	the highest surface oil content	736:766	After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.					
31173831	0	47	theme	shelf-life	64:73	arg1	stability					75:83	shelf-life stability	64:83	shelf-life stability	64:83	Effects of beeswax-carboxymethyl cellulose composite coating on shelf-life stability and intestinal delivery of holy basil essential oil-loaded gelatin microcapsules.					
31173831	4	48	theme	%	893:893	arg1	2B-HBEO-G					928:936	2B-HBEO-G	928:936	2B-HBEO-G	928:936	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	4	48	theme	%	893:893	arg1	microcapsule					914:925	only the 2% beeswax-CMC coated microcapsule	883:925	only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G)	883:937	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	4	49	theme	microcapsule	914:925	arg1	activity					871:878	The antioxidant activity	855:878	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G)	855:937	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	5	50	theme	HBEO	1099:1102	arg1	exposure					1076:1083	exposure	1076:1083	exposure of the loaded HBEO in the simulated gastric fluids	1076:1134	2B-HBEO-G minimized exposure of the loaded HBEO in the simulated gastric fluids and delivered the oil to the distal small intestine.					
31173831	3	51	theme	surface	748:754	arg1	content					760:766	the highest surface oil content	736:766	the highest surface oil content	736:766	After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.					
31173831	2	52	theme	carboxymethyl	542:554	arg1	cellulose					556:564	carboxymethyl cellulose	542:564	carboxymethyl cellulose	542:564	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	1	53	from	oils	217:220	arg1	food					225:228	food	225:228	food	225:228	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	1	54	theme	sensitive	341:349	arg1	materials					351:359	these sensitive materials	335:359	these sensitive materials	335:359	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	4	55	theme	antioxidant	859:869	arg1	activity					871:878	The antioxidant activity	855:878	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G)	855:937	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	1	56	theme	feed	234:237	arg1	additives					239:247	feed additives	234:247	feed additives	234:247	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	1	57	from	additives	239:247	arg1	food					225:228	food	225:228	food	225:228	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	2	58	theme	simple	692:697	arg1	coacervation					699:710	simple coacervation	692:710	simple coacervation	692:710	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	1	59	theme	additives	239:247	arg1	use					200:202	the use	196:202	the use of essential oils in food and feed additives to promote health benefits	196:274	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	4	60	theme	microcapsules	1002:1014	arg1	activities					984:993	the antibacterial activities	966:993	the antibacterial activities of all microcapsules	966:1014	The antioxidant activity of only the 2% beeswax-CMC coated microcapsule (2B-HBEO-G) remained constant, whereas the antibacterial activities of all microcapsules did not significantly change over time.					
31173831	2	61	theme	emulsion-based	485:498	arg1	composite					500:508	emulsion-based composite	485:508	emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating	485:582	This study aimed to investigate the effects of emulsion-based composite from beeswax (1, 2, and 3%) and carboxymethyl cellulose (CMC) for coating on the stability and delivery of holy basil essential oil-loaded gelatin microcapsules (HBEO-G) prepared by simple coacervation.					
31173831	1	62	theme	materials	351:359	arg1	microencapsulation					313:330	microencapsulation	313:330	microencapsulation of these sensitive materials	313:359	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	1	63	theme	growing	175:181	arg1	interests					183:191	growing interests	175:191	growing interests in the use of essential oils in food and feed additives to promote health benefits	175:274	Despite growing interests in the use of essential oils in food and feed additives to promote health benefits, several studies have indicated that microencapsulation of these sensitive materials is necessary to increase their stability and improve their release profiles.					
31173831	3	64	theme	beeswax-CMC	821:831	arg1	microcapsules					840:852	the 3, 1, and 2% beeswax-CMC coated microcapsules	804:852	the 3, 1, and 2% beeswax-CMC coated microcapsules	804:852	After 3-month storage, the highest surface oil content was observed in HBEO-G, followed by the 3, 1, and 2% beeswax-CMC coated microcapsules.					
29852376	3	0	theme	cell	668:671	arg1	response					673:680	stem cell response	663:680	stem cell response	663:680	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	3	1	theme	cell	464:467	arg1	suspensions					469:479	single cell suspensions	457:479	single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels	457:553	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	8	2	dep	relevant	1468:1475	arg1	regenerative					1485:1496	regenerative	1485:1496	regenerative	1485:1496	Moreover, as stem cells are most regenerative if activated from a quiescent state, our results suggest that ex vivo native-like niches that incorporate signaling from neighboring cells may enable the production of clinically relevant, highly regenerative cells.					
29852376	0	3	theme	MSC	66:68	arg1	quiescence					70:79	human MSC quiescence	60:79	human MSC quiescence	60:79	Neighboring cells override 3D hydrogel matrix cues to drive human MSC quiescence.					
29852376	8	4	theme	relevant	1468:1475	arg1	cells					1498:1502	clinically relevant, highly regenerative cells	1457:1502	clinically relevant, highly regenerative cells	1457:1502	Moreover, as stem cells are most regenerative if activated from a quiescent state, our results suggest that ex vivo native-like niches that incorporate signaling from neighboring cells may enable the production of clinically relevant, highly regenerative cells.					
29852376	7	5	contain	have	1117:1120	arg2	implications					1132:1143	important implications	1122:1143	important implications for tissue engineering	1122:1166	Our observations may have important implications for tissue engineering as neighboring cells may act counter to matrix cues provided by scaffolds.					
29852376	7	5	contain	have	1117:1120	arg1	observations					1100:1111	Our observations	1096:1111	Our observations	1096:1111	Our observations may have important implications for tissue engineering as neighboring cells may act counter to matrix cues provided by scaffolds.					
29852376	1	6	theme	stem	149:152	arg1	differentiation					159:173	stem cell differentiation	149:173	stem cell differentiation	149:173	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
29852376	6	7	theme	HD	1030:1031	arg1	cultures					1033:1040	HD cultures	1030:1040	HD cultures	1030:1040	Preventing quiescence precluded the formation of a glycoprotein-rich PCM and forced HD cultures to differentiate in response to hydrogel composition.					
29852376	3	8	theme	hyaluronic	523:532	arg1	hydrogels					545:553	hyaluronic acid-based hydrogels	523:553	hyaluronic acid-based hydrogels	523:553	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	3	9	from	suspensions	469:479	arg1	hydrogels					545:553	hyaluronic acid-based hydrogels	523:553	hyaluronic acid-based hydrogels	523:553	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	3	10	theme	cells	507:511	arg1	suspensions					469:479	single cell suspensions	457:479	single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels	457:553	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	6	11	theme	hydrogel	1074:1081	arg1	composition					1083:1093	hydrogel composition	1074:1093	hydrogel composition	1074:1093	Preventing quiescence precluded the formation of a glycoprotein-rich PCM and forced HD cultures to differentiate in response to hydrogel composition.					
29852376	1	12	theme	native	236:241	arg1	niches					253:258	native stem cell niches	236:258	native stem cell niches	236:258	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
29852376	8	13	theme	quiescent	1309:1317	arg1	state					1319:1323	a quiescent state	1307:1323	a quiescent state	1307:1323	Moreover, as stem cells are most regenerative if activated from a quiescent state, our results suggest that ex vivo native-like niches that incorporate signaling from neighboring cells may enable the production of clinically relevant, highly regenerative cells.					
29852376	8	14	theme	neighboring	1410:1420	arg1	cells					1422:1426	neighboring cells	1410:1426	neighboring cells	1410:1426	Moreover, as stem cells are most regenerative if activated from a quiescent state, our results suggest that ex vivo native-like niches that incorporate signaling from neighboring cells may enable the production of clinically relevant, highly regenerative cells.					
29852376	4	15	theme	HD	713:714	arg1	cultures					717:724	high-density (HD) cultures	699:724	high-density (HD) cultures	699:724	We show that in high-density (HD) cultures, hMSC do not rely on hydrogel cues to guide their fate.					
29852376	1	16	theme	stem	243:246	arg1	niches					253:258	native stem cell niches	236:258	native stem cell niches	236:258	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
29852376	2	17	theme	matrix	299:304	arg1	cues					306:309	matrix cues	299:309	matrix cues	299:309	However, stem cells do not respond to matrix cues in isolation, but rather integrate soluble and non-soluble signals to balance quiescence, self-renewal and differentiation.					
29852376	3	18	theme	mesenchymal	490:500	arg1	cells					507:511	human mesenchymal stem cells	484:511	human mesenchymal stem cells (hMSC)	484:518	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	3	18	theme	mesenchymal	490:500	arg1	hMSC					514:517	hMSC	514:517	hMSC	514:517	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	1	19	theme	cell	154:157	arg1	differentiation					159:173	stem cell differentiation	149:173	stem cell differentiation	149:173	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
29852376	8	20	from	cells	1422:1426	arg1	signaling					1395:1403	signaling	1395:1403	signaling from neighboring cells	1395:1426	Moreover, as stem cells are most regenerative if activated from a quiescent state, our results suggest that ex vivo native-like niches that incorporate signaling from neighboring cells may enable the production of clinically relevant, highly regenerative cells.					
29852376	5	21	theme	quiescent	823:831	arg1	cells					833:837	quiescent cells	823:837	quiescent cells	823:837	Instead, they take on characteristics of quiescent cells and secrete a glycoprotein-rich pericellular matrix (PCM) in response to signaling from neighboring cells.					
29852376	3	22	theme	stem	502:505	arg1	cells					507:511	human mesenchymal stem cells	484:511	human mesenchymal stem cells (hMSC)	484:518	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	3	22	theme	stem	502:505	arg1	hMSC					514:517	hMSC	514:517	hMSC	514:517	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	6	23	theme	PCM	1015:1017	arg1	formation					982:990	the formation	978:990	the formation of a glycoprotein-rich PCM	978:1017	Preventing quiescence precluded the formation of a glycoprotein-rich PCM and forced HD cultures to differentiate in response to hydrogel composition.					
29852376	7	24	theme	neighboring	1171:1181	arg1	cells					1183:1187	neighboring cells	1171:1187	neighboring cells	1171:1187	Our observations may have important implications for tissue engineering as neighboring cells may act counter to matrix cues provided by scaffolds.					
29852376	5	25	theme	glycoprotein-rich	853:869	arg1	matrix					884:889	a glycoprotein-rich pericellular matrix	851:889	a glycoprotein-rich pericellular matrix (PCM)	851:895	Instead, they take on characteristics of quiescent cells and secrete a glycoprotein-rich pericellular matrix (PCM) in response to signaling from neighboring cells.					
29852376	5	25	theme	glycoprotein-rich	853:869	arg1	PCM					892:894	PCM	892:894	PCM	892:894	Instead, they take on characteristics of quiescent cells and secrete a glycoprotein-rich pericellular matrix (PCM) in response to signaling from neighboring cells.					
29852376	6	26	theme	glycoprotein-rich	997:1013	arg1	PCM					1015:1017	a glycoprotein-rich PCM	995:1017	a glycoprotein-rich PCM	995:1017	Preventing quiescence precluded the formation of a glycoprotein-rich PCM and forced HD cultures to differentiate in response to hydrogel composition.					
29852376	0	27	theme	Neighboring	0:10	arg1	cells					12:16	Neighboring cells	0:16	Neighboring cells	0:16	Neighboring cells override 3D hydrogel matrix cues to drive human MSC quiescence.					
29852376	1	28	theme	cell	248:251	arg1	niches					253:258	native stem cell niches	236:258	native stem cell niches	236:258	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
29852376	1	29	theme	Physical	82:89	arg1	properties					91:100	Physical properties	82:100	Physical properties of modifiable hydrogels	82:124	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
29852376	0	30	theme	3D	27:28	arg1	cues					46:49	3D hydrogel matrix cues	27:49	3D hydrogel matrix cues	27:49	Neighboring cells override 3D hydrogel matrix cues to drive human MSC quiescence.					
29852376	5	31	theme	cells	833:837	arg1	characteristics					804:818	characteristics	804:818	characteristics of quiescent cells	804:837	Instead, they take on characteristics of quiescent cells and secrete a glycoprotein-rich pericellular matrix (PCM) in response to signaling from neighboring cells.					
29852376	2	32	theme	stem	270:273	arg1	cells					275:279	stem cells	270:279	stem cells	270:279	However, stem cells do not respond to matrix cues in isolation, but rather integrate soluble and non-soluble signals to balance quiescence, self-renewal and differentiation.					
29852376	2	33	theme	balance	381:387	arg1	quiescence					389:398	balance quiescence	381:398	balance quiescence	381:398	However, stem cells do not respond to matrix cues in isolation, but rather integrate soluble and non-soluble signals to balance quiescence, self-renewal and differentiation.					
29852376	5	34	theme	pericellular	871:882	arg1	matrix					884:889	a glycoprotein-rich pericellular matrix	851:889	a glycoprotein-rich pericellular matrix (PCM)	851:895	Instead, they take on characteristics of quiescent cells and secrete a glycoprotein-rich pericellular matrix (PCM) in response to signaling from neighboring cells.					
29852376	5	34	theme	pericellular	871:882	arg1	PCM					892:894	PCM	892:894	PCM	892:894	Instead, they take on characteristics of quiescent cells and secrete a glycoprotein-rich pericellular matrix (PCM) in response to signaling from neighboring cells.					
29852376	4	35	theme	high-density	699:710	arg1	cultures					717:724	high-density (HD) cultures	699:724	high-density (HD) cultures	699:724	We show that in high-density (HD) cultures, hMSC do not rely on hydrogel cues to guide their fate.					
29852376	5	36	from	cells	939:943	arg1	signaling					912:920	signaling	912:920	signaling from neighboring cells	912:943	Instead, they take on characteristics of quiescent cells and secrete a glycoprotein-rich pericellular matrix (PCM) in response to signaling from neighboring cells.					
29852376	1	37	theme	akin	185:188	arg1	role					180:183	a role	178:183	a role akin to that played by the extracellular matrix in native stem cell niches	178:258	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
29852376	0	38	theme	matrix	39:44	arg1	cues					46:49	3D hydrogel matrix cues	27:49	3D hydrogel matrix cues	27:49	Neighboring cells override 3D hydrogel matrix cues to drive human MSC quiescence.					
29852376	3	39	theme	single	457:462	arg1	suspensions					469:479	single cell suspensions	457:479	single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels	457:553	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	7	40	theme	important	1122:1130	arg1	implications					1132:1143	important implications	1122:1143	important implications for tissue engineering	1122:1166	Our observations may have important implications for tissue engineering as neighboring cells may act counter to matrix cues provided by scaffolds.					
29852376	4	41	theme	hydrogel	747:754	arg1	cues					756:759	hydrogel cues	747:759	hydrogel cues	747:759	We show that in high-density (HD) cultures, hMSC do not rely on hydrogel cues to guide their fate.					
29852376	2	42	theme	non-soluble	358:368	arg1	differentiation					418:432	differentiation	418:432	differentiation	418:432	However, stem cells do not respond to matrix cues in isolation, but rather integrate soluble and non-soluble signals to balance quiescence, self-renewal and differentiation.					
29852376	2	42	theme	non-soluble	358:368	arg1	self-renewal					401:412	self-renewal	401:412	self-renewal	401:412	However, stem cells do not respond to matrix cues in isolation, but rather integrate soluble and non-soluble signals to balance quiescence, self-renewal and differentiation.					
29852376	2	42	theme	non-soluble	358:368	arg1	signals					370:376	soluble and non-soluble signals	346:376	soluble and non-soluble signals to balance quiescence	346:398	However, stem cells do not respond to matrix cues in isolation, but rather integrate soluble and non-soluble signals to balance quiescence, self-renewal and differentiation.					
29852376	0	43	theme	hydrogel	30:37	arg1	cues					46:49	3D hydrogel matrix cues	27:49	3D hydrogel matrix cues	27:49	Neighboring cells override 3D hydrogel matrix cues to drive human MSC quiescence.					
29852376	8	44	theme	stem	1256:1259	arg1	cells					1261:1265	stem cells	1256:1265	stem cells	1256:1265	Moreover, as stem cells are most regenerative if activated from a quiescent state, our results suggest that ex vivo native-like niches that incorporate signaling from neighboring cells may enable the production of clinically relevant, highly regenerative cells.					
29852376	3	45	theme	high	558:561	arg1	densities					571:579	high and low densities	558:579	high and low densities	558:579	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	5	46	dep	signaling	912:920	arg1	response					900:907	response	900:907	response	900:907	Instead, they take on characteristics of quiescent cells and secrete a glycoprotein-rich pericellular matrix (PCM) in response to signaling from neighboring cells.					
29852376	7	47	theme	tissue	1149:1154	arg1	engineering					1156:1166	tissue engineering	1149:1166	tissue engineering	1149:1166	Our observations may have important implications for tissue engineering as neighboring cells may act counter to matrix cues provided by scaffolds.					
29852376	3	48	theme	acid-based	534:543	arg1	hydrogels					545:553	hyaluronic acid-based hydrogels	523:553	hyaluronic acid-based hydrogels	523:553	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	8	49	theme	cells	1498:1502	arg1	production					1443:1452	the production	1439:1452	the production of clinically relevant, highly regenerative cells	1439:1502	Moreover, as stem cells are most regenerative if activated from a quiescent state, our results suggest that ex vivo native-like niches that incorporate signaling from neighboring cells may enable the production of clinically relevant, highly regenerative cells.					
29852376	1	50	theme	modifiable	105:114	arg1	hydrogels					116:124	modifiable hydrogels	105:124	modifiable hydrogels	105:124	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
29852376	2	51	theme	soluble	346:352	arg1	differentiation					418:432	differentiation	418:432	differentiation	418:432	However, stem cells do not respond to matrix cues in isolation, but rather integrate soluble and non-soluble signals to balance quiescence, self-renewal and differentiation.					
29852376	2	51	theme	soluble	346:352	arg1	self-renewal					401:412	self-renewal	401:412	self-renewal	401:412	However, stem cells do not respond to matrix cues in isolation, but rather integrate soluble and non-soluble signals to balance quiescence, self-renewal and differentiation.					
29852376	2	51	theme	soluble	346:352	arg1	signals					370:376	soluble and non-soluble signals	346:376	soluble and non-soluble signals to balance quiescence	346:398	However, stem cells do not respond to matrix cues in isolation, but rather integrate soluble and non-soluble signals to balance quiescence, self-renewal and differentiation.					
29852376	3	52	theme	non-matrix-mediated	625:643	arg1	cues					645:648	non-matrix-mediated cues	625:648	non-matrix-mediated cues	625:648	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	1	53	theme	hydrogels	116:124	arg1	properties					91:100	Physical properties	82:100	Physical properties of modifiable hydrogels	82:124	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
29852376	5	54	theme	neighboring	927:937	arg1	cells					939:943	neighboring cells	927:943	neighboring cells	927:943	Instead, they take on characteristics of quiescent cells and secrete a glycoprotein-rich pericellular matrix (PCM) in response to signaling from neighboring cells.					
29852376	3	55	theme	cues	645:648	arg1	contributions					596:608	the contributions	592:608	the contributions of matrix- and non-matrix-mediated cues in directing stem cell response	592:680	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	8	56	theme	native-like	1359:1369	arg1	niches					1371:1376	ex vivo native-like niches	1351:1376	ex vivo native-like niches that incorporate signaling from neighboring cells	1351:1426	Moreover, as stem cells are most regenerative if activated from a quiescent state, our results suggest that ex vivo native-like niches that incorporate signaling from neighboring cells may enable the production of clinically relevant, highly regenerative cells.					
29852376	3	57	theme	matrix-	613:619	arg1	contributions					596:608	the contributions	592:608	the contributions of matrix- and non-matrix-mediated cues in directing stem cell response	592:680	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	8	58	theme	ex vivo	1351:1357	arg1	niches					1371:1376	ex vivo native-like niches	1351:1376	ex vivo native-like niches that incorporate signaling from neighboring cells	1351:1426	Moreover, as stem cells are most regenerative if activated from a quiescent state, our results suggest that ex vivo native-like niches that incorporate signaling from neighboring cells may enable the production of clinically relevant, highly regenerative cells.					
29852376	7	59	theme	matrix	1208:1213	arg1	cues					1215:1218	matrix cues	1208:1218	matrix cues provided by scaffolds	1208:1240	Our observations may have important implications for tissue engineering as neighboring cells may act counter to matrix cues provided by scaffolds.					
29852376	0	60	theme	human	60:64	arg1	quiescence					70:79	human MSC quiescence	60:79	human MSC quiescence	60:79	Neighboring cells override 3D hydrogel matrix cues to drive human MSC quiescence.					
29852376	3	61	theme	stem	663:666	arg1	response					673:680	stem cell response	663:680	stem cell response	663:680	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	3	62	theme	low	567:569	arg1	densities					571:579	high and low densities	558:579	high and low densities	558:579	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	3	63	theme	human	484:488	arg1	cells					507:511	human mesenchymal stem cells	484:511	human mesenchymal stem cells (hMSC)	484:518	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	3	63	theme	human	484:488	arg1	hMSC					514:517	hMSC	514:517	hMSC	514:517	Here, we encapsulated single cell suspensions of human mesenchymal stem cells (hMSC) in hyaluronic acid-based hydrogels at high and low densities to unravel the contributions of matrix- and non-matrix-mediated cues in directing stem cell response.					
29852376	1	64	theme	extracellular	212:224	arg1	matrix					226:231	the extracellular matrix	208:231	the extracellular matrix	208:231	Physical properties of modifiable hydrogels can be tuned to direct stem cell differentiation in a role akin to that played by the extracellular matrix in native stem cell niches.					
30193400	8	0	theme	soil	1280:1283	arg1	enzymes					1285:1291	soil enzymes	1280:1291	soil enzymes	1280:1291	Seed mucilage directly improved seedling emergence and indirectly interacted with the soil microbial community through strengthening fungal-bacterial interactions and providing favourable environment for soil enzymes to affect seedling emergence.					
30193400	2	1	theme	seedling	337:344	arg1	emergence					346:354	seedling emergence	337:354	seedling emergence	337:354	Soil microbes participate in plant growth and development, but information is lacking with regard to the role of microbes on seedling emergence.					
30193400	3	2	from	emergence	523:531	arg1	dune					580:583	the desert sand dune	564:583	the desert sand dune	564:583	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	8	3	theme	microbial	1167:1175	arg1	community					1177:1185	the soil microbial community	1158:1185	the soil microbial community	1158:1185	Seed mucilage directly improved seedling emergence and indirectly interacted with the soil microbial community through strengthening fungal-bacterial interactions and providing favourable environment for soil enzymes to affect seedling emergence.					
30193400	9	4	theme	seed	1439:1442	arg1	mucilage					1444:1451	seed mucilage	1439:1451	seed mucilage	1439:1451	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	8	5	theme	seedling	1108:1115	arg1	emergence					1117:1125	seedling emergence	1108:1125	seedling emergence	1108:1125	Seed mucilage directly improved seedling emergence and indirectly interacted with the soil microbial community through strengthening fungal-bacterial interactions and providing favourable environment for soil enzymes to affect seedling emergence.					
30193400	2	6	from	role	317:320	arg1	emergence					346:354	seedling emergence	337:354	seedling emergence	337:354	Soil microbes participate in plant growth and development, but information is lacking with regard to the role of microbes on seedling emergence.					
30193400	1	7	theme	critical	158:165	arg1	stage					167:171	a critical stage	156:171	a critical stage in the establishment of desert plants	156:209	Seedling emergence is a critical stage in the establishment of desert plants.					
30193400	1	7	theme	critical	158:165	arg1	emergence					143:151	Seedling emergence	134:151	Seedling emergence	134:151	Seedling emergence is a critical stage in the establishment of desert plants.					
30193400	5	8	theme	enzyme	747:752	arg1	activities					754:763	soil enzyme activities	742:763	soil enzyme activities	742:763	Mucilage increased soil enzyme activities and fungal-bacterial interactions.					
30193400	9	9	theme	soil	1410:1413	arg1	community					1425:1433	soil microbial community	1410:1433	soil microbial community	1410:1433	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	7	10	theme	keystone	938:945	arg1	species					947:953	the keystone species	934:953	the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen	934:1073	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	7	10	theme	keystone	938:945	arg1	Members					879:885	Members	879:885	Members of the phyla Firmicutes and Glomeromycota	879:927	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	4	11	theme	Fungal	586:591	arg1	composition					617:627	Fungal and bacterial community composition	586:627	Fungal and bacterial community composition	586:627	Fungal and bacterial community composition and diversity and fungal-bacterial interactions were changed by both captan and streptomycin.					
30193400	0	12	theme	seedling	93:100	arg1	emergence					102:110	seedling emergence	93:110	seedling emergence on desert sand dunes	93:131	Seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence on desert sand dunes.					
30193400	3	13	dep	biocides	372:379	arg1	captan					382:387	captan	382:387	captan	382:387	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	3	13	dep	biocides	372:379	arg1	biocides					372:379	the biocides	368:379	the biocides (captan and streptomycin)	368:405	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	3	13	dep	biocides	372:379	arg1	streptomycin					393:404	streptomycin	393:404	streptomycin	393:404	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	3	14	theme	Artemisia	536:544	arg1	sphaerocephala					546:559	Artemisia sphaerocephala	536:559	Artemisia sphaerocephala	536:559	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	3	15	theme	microbial	455:463	arg1	community					465:473	soil microbial community	450:473	soil microbial community	450:473	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	0	16	theme	desert	115:120	arg1	sand					122:125	desert sand	115:125	desert sand dunes	115:131	Seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence on desert sand dunes.					
30193400	7	17	theme	young	1035:1039	arg1	seedlings					1041:1049	young seedlings	1035:1049	young seedlings	1035:1049	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	3	18	theme	seedling	514:521	arg1	emergence					523:531	seedling emergence	514:531	seedling emergence of Artemisia sphaerocephala on the desert sand dune	514:583	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	5	19	theme	fungal-bacterial	769:784	arg1	interactions					786:797	fungal-bacterial interactions	769:797	fungal-bacterial interactions	769:797	Mucilage increased soil enzyme activities and fungal-bacterial interactions.					
30193400	9	20	theme	successful	1475:1484	arg1	establishment					1486:1498	successful establishment	1475:1498	successful establishment of populations of desert plants	1475:1530	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	6	21	dep	streptomycin	842:853	arg1	treatment					868:876	treatment	868:876	treatment	868:876	Highest seedling emergence occurred under streptomycin and mucilage treatment.					
30193400	8	22	theme	seedling	1303:1310	arg1	emergence					1312:1320	seedling emergence	1303:1320	seedling emergence	1303:1320	Seed mucilage directly improved seedling emergence and indirectly interacted with the soil microbial community through strengthening fungal-bacterial interactions and providing favourable environment for soil enzymes to affect seedling emergence.					
30193400	8	23	theme	soil	1162:1165	arg1	community					1177:1185	the soil microbial community	1158:1185	the soil microbial community	1158:1185	Seed mucilage directly improved seedling emergence and indirectly interacted with the soil microbial community through strengthening fungal-bacterial interactions and providing favourable environment for soil enzymes to affect seedling emergence.					
30193400	8	24	theme	Seed	1076:1079	arg1	mucilage					1081:1088	Seed mucilage	1076:1088	Seed mucilage	1076:1088	Seed mucilage directly improved seedling emergence and indirectly interacted with the soil microbial community through strengthening fungal-bacterial interactions and providing favourable environment for soil enzymes to affect seedling emergence.					
30193400	4	25	theme	fungal-bacterial	647:662	arg1	interactions					664:675	fungal-bacterial interactions	647:675	fungal-bacterial interactions	647:675	Fungal and bacterial community composition and diversity and fungal-bacterial interactions were changed by both captan and streptomycin.					
30193400	0	26	theme	Seed	0:3	arg1	mucilage					5:12	Seed mucilage	0:12	Seed mucilage	0:12	Seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence on desert sand dunes.					
30193400	9	27	theme	comprehensive	1344:1356	arg1	understanding					1358:1370	a comprehensive understanding	1342:1370	a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune	1342:1568	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	3	28	theme	desert	568:573	arg1	sand					575:578	desert sand	568:578	the desert sand dune	564:583	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	9	29	theme	populations	1503:1513	arg1	establishment					1486:1498	successful establishment	1475:1498	successful establishment of populations of desert plants	1475:1530	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	3	30	theme	sphaerocephala	546:559	arg1	emergence					523:531	seedling emergence	514:531	seedling emergence of Artemisia sphaerocephala on the desert sand dune	514:583	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	7	31	theme	phyla	894:898	arg1	species					947:953	the keystone species	934:953	the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen	934:1073	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	7	31	theme	phyla	894:898	arg1	Members					879:885	Members	879:885	Members of the phyla Firmicutes and Glomeromycota	879:927	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	7	31	theme	phyla	894:898	arg1	Glomeromycota					915:927	Glomeromycota	915:927	Glomeromycota	915:927	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	7	31	theme	phyla	894:898	arg1	Firmicutes					900:909	Firmicutes	900:909	Firmicutes	900:909	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	0	32	theme	sand	122:125	arg1	dunes					127:131	desert sand dunes	115:131	desert sand dunes	115:131	Seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence on desert sand dunes.					
30193400	9	33	theme	mechanisms	1390:1399	arg1	understanding					1358:1370	a comprehensive understanding	1342:1370	a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune	1342:1568	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	1	34	theme	desert	197:202	arg1	plants					204:209	desert plants	197:209	desert plants	197:209	Seedling emergence is a critical stage in the establishment of desert plants.					
30193400	0	35	theme	microbial	34:42	arg1	community					44:52	soil microbial community	29:52	soil microbial community	29:52	Seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence on desert sand dunes.					
30193400	9	36	theme	desert	1518:1523	arg1	plants					1525:1530	desert plants	1518:1530	desert plants	1518:1530	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	3	37	theme	seed	421:424	arg1	mucilage					426:433	seed mucilage	421:433	seed mucilage	421:433	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	1	38	theme	plants	204:209	arg1	establishment					180:192	the establishment	176:192	the establishment of desert plants	176:209	Seedling emergence is a critical stage in the establishment of desert plants.					
30193400	0	39	theme	soil	29:32	arg1	community					44:52	soil microbial community	29:52	soil microbial community	29:52	Seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence on desert sand dunes.					
30193400	9	40	theme	plants	1525:1530	arg1	populations					1503:1513	populations	1503:1513	populations of desert plants	1503:1530	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	5	41	theme	soil	742:745	arg1	activities					754:763	soil enzyme activities	742:763	soil enzyme activities	742:763	Mucilage increased soil enzyme activities and fungal-bacterial interactions.					
30193400	9	42	theme	regulatory	1379:1388	arg1	mechanisms					1390:1399	the regulatory mechanisms	1375:1399	the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune	1375:1568	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	6	43	theme	seedling	808:815	arg1	emergence					817:825	Highest seedling emergence	800:825	Highest seedling emergence	800:825	Highest seedling emergence occurred under streptomycin and mucilage treatment.					
30193400	3	44	theme	physiochemical	479:492	arg1	processes					494:502	physiochemical processes	479:502	physiochemical processes	479:502	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	8	45	theme	favourable	1253:1262	arg1	environment					1264:1274	favourable environment	1253:1274	favourable environment for soil enzymes to affect seedling emergence	1253:1320	Seed mucilage directly improved seedling emergence and indirectly interacted with the soil microbial community through strengthening fungal-bacterial interactions and providing favourable environment for soil enzymes to affect seedling emergence.					
30193400	3	46	theme	sand	575:578	arg1	dune					580:583	the desert sand dune	564:583	the desert sand dune	564:583	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	6	47	theme	Highest	800:806	arg1	emergence					817:825	Highest seedling emergence	800:825	Highest seedling emergence	800:825	Highest seedling emergence occurred under streptomycin and mucilage treatment.					
30193400	2	48	theme	microbes	325:332	arg1	role					317:320	the role	313:320	the role of microbes on seedling emergence	313:354	Soil microbes participate in plant growth and development, but information is lacking with regard to the role of microbes on seedling emergence.					
30193400	8	49	theme	fungal-bacterial	1209:1224	arg1	interactions					1226:1237	fungal-bacterial interactions	1209:1237	fungal-bacterial interactions	1209:1237	Seed mucilage directly improved seedling emergence and indirectly interacted with the soil microbial community through strengthening fungal-bacterial interactions and providing favourable environment for soil enzymes to affect seedling emergence.					
30193400	2	50	theme	Soil	212:215	arg1	microbes					217:224	Soil microbes	212:224	Soil microbes	212:224	Soil microbes participate in plant growth and development, but information is lacking with regard to the role of microbes on seedling emergence.					
30193400	7	51	theme	seedling	987:994	arg1	emergence					996:1004	A. sphaerocephala seedling emergence	969:1004	A. sphaerocephala seedling emergence	969:1004	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	9	52	theme	barren	1539:1544	arg1	dune					1565:1568	the barren and stressful sand dune	1535:1568	the barren and stressful sand dune	1535:1568	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	3	53	theme	soil	450:453	arg1	community					465:473	soil microbial community	450:473	soil microbial community	450:473	We applied the biocides (captan and streptomycin) to assess how seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence of Artemisia sphaerocephala on the desert sand dune.					
30193400	4	54	theme	bacterial	597:605	arg1	composition					617:627	Fungal and bacterial community composition	586:627	Fungal and bacterial community composition	586:627	Fungal and bacterial community composition and diversity and fungal-bacterial interactions were changed by both captan and streptomycin.					
30193400	0	55	from	emergence	102:110	arg1	dunes					127:131	desert sand dunes	115:131	desert sand dunes	115:131	Seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence on desert sand dunes.					
30193400	7	56	dep	Members	879:885	arg1	Members					879:885	Members	879:885	Members of the phyla Firmicutes and Glomeromycota	879:927	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	7	56	dep	Members	879:885	arg1	Glomeromycota					915:927	Glomeromycota	915:927	Glomeromycota	915:927	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	7	56	dep	Members	879:885	arg1	Firmicutes					900:909	Firmicutes	900:909	Firmicutes	900:909	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	1	57	theme	Seedling	134:141	arg1	stage					167:171	a critical stage	156:171	a critical stage in the establishment of desert plants	156:209	Seedling emergence is a critical stage in the establishment of desert plants.					
30193400	1	57	theme	Seedling	134:141	arg1	emergence					143:151	Seedling emergence	134:151	Seedling emergence	134:151	Seedling emergence is a critical stage in the establishment of desert plants.					
30193400	9	58	theme	stressful	1550:1558	arg1	dune					1565:1568	the barren and stressful sand dune	1535:1568	the barren and stressful sand dune	1535:1568	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	9	59	theme	microbial	1415:1423	arg1	community					1425:1433	soil microbial community	1410:1433	soil microbial community	1410:1433	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	7	60	theme	seedlings	1041:1049	arg1	resistance					1021:1030	resistance	1021:1030	resistance of young seedlings to drought and pathogen	1021:1073	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	7	61	theme	A. sphaerocephala	969:985	arg1	emergence					996:1004	A. sphaerocephala seedling emergence	969:1004	A. sphaerocephala seedling emergence	969:1004	Members of the phyla Firmicutes and Glomeromycota were the keystone species that improved A. sphaerocephala seedling emergence, by increasing resistance of young seedlings to drought and pathogen.					
30193400	9	62	theme	sand	1560:1563	arg1	dune					1565:1568	the barren and stressful sand dune	1535:1568	the barren and stressful sand dune	1535:1568	Our study provides a comprehensive understanding of the regulatory mechanisms by which soil microbial community and seed mucilage interactively promote successful establishment of populations of desert plants on the barren and stressful sand dune.					
30193400	0	63	theme	physiochemical	58:71	arg1	processes					73:81	physiochemical processes	58:81	physiochemical processes	58:81	Seed mucilage interacts with soil microbial community and physiochemical processes to affect seedling emergence on desert sand dunes.					
30193400	2	64	theme	plant	241:245	arg1	growth					247:252	plant growth	241:252	plant growth	241:252	Soil microbes participate in plant growth and development, but information is lacking with regard to the role of microbes on seedling emergence.					
30193400	1	65	from	stage	167:171	arg1	establishment					180:192	the establishment	176:192	the establishment of desert plants	176:209	Seedling emergence is a critical stage in the establishment of desert plants.					
30193400	4	66	theme	community	607:615	arg1	composition					617:627	Fungal and bacterial community composition	586:627	Fungal and bacterial community composition	586:627	Fungal and bacterial community composition and diversity and fungal-bacterial interactions were changed by both captan and streptomycin.					
29606425	1	0	theme	healthy	153:159	arg1	pattern					173:179	its healthy nutritional pattern	149:179	its healthy nutritional pattern	149:179	Banana flour has been recognized as functional ingredient, owing to its healthy nutritional pattern.					
29606425	1	1	theme	nutritional	161:171	arg1	pattern					173:179	its healthy nutritional pattern	149:179	its healthy nutritional pattern	149:179	Banana flour has been recognized as functional ingredient, owing to its healthy nutritional pattern.					
29606425	3	2	theme	physiochemical	377:390	arg1	characteristics					578:592	physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics	377:592	physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour	377:608	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	4	3	theme	3rd	811:813	arg1	stages					815:820	2nd and 3rd stages	803:820	2nd and 3rd stages	803:820	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	6	4	from	stage	1147:1151	arg1	characteristics					1098:1112	the physicochemical and nutritional characteristics	1062:1112	the physicochemical and nutritional characteristics of banana flour at each ripening stage	1062:1151	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	6	4	from	stage	1147:1151	arg1	flour					1124:1128	banana flour	1117:1128	banana flour at each ripening stage	1117:1151	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	5	5	theme	antioxidant	1012:1022	arg1	activity					1024:1031	antioxidant activity	1012:1031	antioxidant activity	1012:1031	Between those stages, a significant decrease in total and resistant starch was produced together with an increase in total phenolic content and antioxidant activity.					
29606425	3	6	theme	banana	597:602	arg1	flour					604:608	banana flour	597:608	banana flour	597:608	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	6	7	theme	industrial	1194:1203	arg1	application					1205:1215	the industrial application	1190:1215	the industrial application	1190:1215	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	4	8	theme	protein	675:681	arg1	content					683:689	protein content	675:689	protein content	675:689	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	1	9	theme	Banana	81:86	arg1	flour					88:92	Banana flour	81:92	Banana flour	81:92	Banana flour has been recognized as functional ingredient, owing to its healthy nutritional pattern.					
29606425	1	9	theme	Banana	81:86	arg1	ingredient					128:137	functional ingredient	117:137	functional ingredient	117:137	Banana flour has been recognized as functional ingredient, owing to its healthy nutritional pattern.					
29606425	6	10	theme	ripening	1138:1145	arg1	stage					1147:1151	each ripening stage	1133:1151	each ripening stage	1133:1151	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	3	11	theme	rheological	437:447	arg1	properties					449:458	rheological properties	437:458	rheological properties	437:458	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	6	12	from	characteristics	1098:1112	arg1	stage					1147:1151	each ripening stage	1133:1151	each ripening stage	1133:1151	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	3	13	theme	hydration	415:423	arg1	properties					425:434	hydration properties	415:434	hydration properties	415:434	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	5	14	from	increase	973:980	arg1	content					1000:1006	total phenolic content	985:1006	total phenolic content	985:1006	Between those stages, a significant decrease in total and resistant starch was produced together with an increase in total phenolic content and antioxidant activity.					
29606425	5	14	from	increase	973:980	arg1	activity					1024:1031	antioxidant activity	1012:1031	antioxidant activity	1012:1031	Between those stages, a significant decrease in total and resistant starch was produced together with an increase in total phenolic content and antioxidant activity.					
29606425	4	15	theme	2nd	803:805	arg1	stages					815:820	2nd and 3rd stages	803:820	2nd and 3rd stages	803:820	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	4	16	theme	apparent	724:731	arg1	content					741:747	apparent amylose content	724:747	apparent amylose content	724:747	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	0	17	theme	Physicochemical	0:14	arg1	characteristics					32:46	Physicochemical and nutritional characteristics	0:46	Physicochemical and nutritional characteristics of banana	0:56	Physicochemical and nutritional characteristics of banana flour during ripening.					
29606425	3	18	theme	initial	617:623	arg1	stages					639:644	the initial four ripening stages	613:644	the initial four ripening stages	613:644	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	4	19	from	decrease	695:702	arg1	carbohydrate					707:718	carbohydrate	707:718	carbohydrate	707:718	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	4	19	from	decrease	695:702	arg1	content					683:689	protein content	675:689	protein content	675:689	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	4	19	from	decrease	695:702	arg1	content					741:747	apparent amylose content	724:747	apparent amylose content	724:747	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	3	20	theme	structural	464:473	arg1	characteristic					475:488	structural characteristic	464:488	structural characteristic	464:488	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	21	theme	chemical	393:400	arg1	composition					402:412	chemical composition	393:412	chemical composition	393:412	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	6	22	from	selection	1167:1175	arg1	application					1205:1215	the industrial application	1190:1215	the industrial application	1190:1215	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	3	23	theme	flour	604:608	arg1	characteristics					578:592	physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics	377:592	physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour	377:608	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	2	24	dep	show	226:229	arg1	available					283:291	available	283:291	available	283:291	Nevertheless, unripe and ripe banana flours show different characteristics and scarce information is available about changes undergone during banana ripening.					
29606425	0	25	theme	nutritional	20:30	arg1	characteristics					32:46	Physicochemical and nutritional characteristics	0:46	Physicochemical and nutritional characteristics of banana	0:56	Physicochemical and nutritional characteristics of banana flour during ripening.					
29606425	5	26	theme	total	985:989	arg1	content					1000:1006	total phenolic content	985:1006	total phenolic content	985:1006	Between those stages, a significant decrease in total and resistant starch was produced together with an increase in total phenolic content and antioxidant activity.					
29606425	3	27	from	changes	366:372	arg1	characteristics					578:592	physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics	377:592	physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour	377:608	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	28	theme	resistant	508:516	arg1	content					525:531	resistant starch content	508:531	resistant starch content	508:531	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	0	29	theme	banana	51:56	arg1	characteristics					32:46	Physicochemical and nutritional characteristics	0:46	Physicochemical and nutritional characteristics of banana	0:56	Physicochemical and nutritional characteristics of banana flour during ripening.					
29606425	6	30	theme	flour	1124:1128	arg1	characteristics					1098:1112	the physicochemical and nutritional characteristics	1062:1112	the physicochemical and nutritional characteristics of banana flour at each ripening stage	1062:1151	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	6	31	theme	characteristics	1098:1112	arg1	knowledge					1049:1057	the knowledge	1045:1057	the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage	1045:1151	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	2	32	theme	different	231:239	arg1	characteristics					241:255	different characteristics	231:255	different characteristics	231:255	Nevertheless, unripe and ripe banana flours show different characteristics and scarce information is available about changes undergone during banana ripening.					
29606425	5	33	theme	significant	892:902	arg1	decrease					904:911	a significant decrease	890:911	a significant decrease in total and resistant starch	890:941	Between those stages, a significant decrease in total and resistant starch was produced together with an increase in total phenolic content and antioxidant activity.					
29606425	3	34	theme	nutritional	495:505	arg1	characteristics					578:592	physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics	377:592	physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour	377:608	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	5	35	theme	resistant	926:934	arg1	starch					936:941	resistant starch	926:941	resistant starch	926:941	Between those stages, a significant decrease in total and resistant starch was produced together with an increase in total phenolic content and antioxidant activity.					
29606425	6	36	theme	nutritional	1086:1096	arg1	characteristics					1098:1112	the physicochemical and nutritional characteristics	1062:1112	the physicochemical and nutritional characteristics of banana flour at each ripening stage	1062:1151	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	1	37	theme	functional	117:126	arg1	flour					88:92	Banana flour	81:92	Banana flour	81:92	Banana flour has been recognized as functional ingredient, owing to its healthy nutritional pattern.					
29606425	1	37	theme	functional	117:126	arg1	ingredient					128:137	functional ingredient	117:137	functional ingredient	117:137	Banana flour has been recognized as functional ingredient, owing to its healthy nutritional pattern.					
29606425	5	38	theme	phenolic	991:998	arg1	content					1000:1006	total phenolic content	985:1006	total phenolic content	985:1006	Between those stages, a significant decrease in total and resistant starch was produced together with an increase in total phenolic content and antioxidant activity.					
29606425	2	39	theme	banana	212:217	arg1	flours					219:224	unripe and ripe banana flours	196:224	unripe and ripe banana flours	196:224	Nevertheless, unripe and ripe banana flours show different characteristics and scarce information is available about changes undergone during banana ripening.					
29606425	3	40	theme	ripening	630:637	arg1	stages					639:644	the initial four ripening stages	613:644	the initial four ripening stages	613:644	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	41	theme	phenolic	534:541	arg1	compound					543:550	phenolic compound	534:550	phenolic compound	534:550	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	42	dep	physiochemical	377:390	arg1	activity					568:575	antioxidant activity	556:575	antioxidant activity	556:575	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	42	dep	physiochemical	377:390	arg1	characteristic					475:488	structural characteristic	464:488	structural characteristic	464:488	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	42	dep	physiochemical	377:390	arg1	content					525:531	resistant starch content	508:531	resistant starch content	508:531	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	42	dep	physiochemical	377:390	arg1	properties					425:434	hydration properties	415:434	hydration properties	415:434	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	42	dep	physiochemical	377:390	arg1	properties					449:458	rheological properties	437:458	rheological properties	437:458	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	42	dep	physiochemical	377:390	arg1	composition					402:412	chemical composition	393:412	chemical composition	393:412	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	3	42	dep	physiochemical	377:390	arg1	compound					543:550	phenolic compound	534:550	phenolic compound	534:550	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	2	43	theme	ripe	207:210	arg1	flours					219:224	unripe and ripe banana flours	196:224	unripe and ripe banana flours	196:224	Nevertheless, unripe and ripe banana flours show different characteristics and scarce information is available about changes undergone during banana ripening.					
29606425	4	44	theme	climacteric	834:844	arg1	peak					846:849	a climacteric peak	832:849	a climacteric peak during ripening	832:865	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	6	45	theme	physicochemical	1066:1080	arg1	characteristics					1098:1112	the physicochemical and nutritional characteristics	1062:1112	the physicochemical and nutritional characteristics of banana flour at each ripening stage	1062:1151	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	3	46	theme	starch	518:523	arg1	content					525:531	resistant starch content	508:531	resistant starch content	508:531	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	2	47	theme	banana	324:329	arg1	ripening					331:338	banana ripening	324:338	banana ripening	324:338	Nevertheless, unripe and ripe banana flours show different characteristics and scarce information is available about changes undergone during banana ripening.					
29606425	4	48	theme	significant	651:661	arg1	increase					663:670	The significant increase	647:670	The significant increase in protein content	647:689	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	6	49	theme	better	1160:1165	arg1	selection					1167:1175	better selection	1160:1175	better selection depending on the industrial application	1160:1215	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	4	50	from	reduction	762:770	arg1	properties					783:792	pasting properties	775:792	pasting properties	775:792	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	4	51	theme	pasting	775:781	arg1	properties					783:792	pasting properties	775:792	pasting properties	775:792	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	2	52	theme	unripe	196:201	arg1	flours					219:224	unripe and ripe banana flours	196:224	unripe and ripe banana flours	196:224	Nevertheless, unripe and ripe banana flours show different characteristics and scarce information is available about changes undergone during banana ripening.					
29606425	2	53	theme	scarce	261:266	arg1	information					268:278	scarce information	261:278	scarce information	261:278	Nevertheless, unripe and ripe banana flours show different characteristics and scarce information is available about changes undergone during banana ripening.					
29606425	6	54	theme	banana	1117:1122	arg1	flour					1124:1128	banana flour	1117:1128	banana flour at each ripening stage	1117:1151	Therefore, the knowledge of the physicochemical and nutritional characteristics of banana flour at each ripening stage allows better selection depending on the industrial application.					
29606425	3	55	theme	antioxidant	556:566	arg1	activity					568:575	antioxidant activity	556:575	antioxidant activity	556:575	This study evaluates the changes on physiochemical (chemical composition, hydration properties, rheological properties and structural characteristic) and nutritional (resistant starch content, phenolic compound and antioxidant activity) characteristics of banana flour at the initial four ripening stages.					
29606425	4	56	from	increase	663:670	arg1	carbohydrate					707:718	carbohydrate	707:718	carbohydrate	707:718	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	4	56	from	increase	663:670	arg1	content					683:689	protein content	675:689	protein content	675:689	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	4	56	from	increase	663:670	arg1	content					741:747	apparent amylose content	724:747	apparent amylose content	724:747	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29606425	5	57	from	decrease	904:911	arg1	total					916:920	total	916:920	total	916:920	Between those stages, a significant decrease in total and resistant starch was produced together with an increase in total phenolic content and antioxidant activity.					
29606425	5	57	from	decrease	904:911	arg1	starch					936:941	resistant starch	926:941	resistant starch	926:941	Between those stages, a significant decrease in total and resistant starch was produced together with an increase in total phenolic content and antioxidant activity.					
29606425	4	58	theme	amylose	733:739	arg1	content					741:747	apparent amylose content	724:747	apparent amylose content	724:747	The significant increase in protein content and decrease in carbohydrate and apparent amylose content, besides the reduction in pasting properties, between 2nd and 3rd stages suggested a climacteric peak during ripening.					
29352487	10	0	theme	motile	1579:1584	arg1	sperm					1586:1590	motile sperm	1579:1590	motile sperm	1579:1590	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	1	theme	positive	1424:1431	arg1	correlations					1433:1444	significant positive correlations	1412:1444	significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm	1412:1707	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	2	2	theme	membrane	375:382	arg1	ATPase					389:394	plasma membrane Ca2+ ATPase	368:394	plasma membrane Ca2+ ATPase isoform (PMCA2)	368:410	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29352487	10	3	theme	straight-line	1615:1627	arg1	velocity					1629:1636	straight-line velocity	1615:1636	straight-line velocity	1615:1636	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	5	4	theme	low	705:707	arg1	optimal					764:770	optimal	764:770	optimal	764:770	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	5	4	theme	low	705:707	arg1	concentration					709:721	A low concentration	703:721	A low concentration of sucrose plus trehalose (S15 T15 )	703:758	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	5	4	theme	low	705:707	arg1	T15					754:756	S15 T15	750:756	S15 T15	750:756	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	2	5	theme	membrane-impermeable	306:325	arg1	cryoprotectants					327:341	membrane-impermeable cryoprotectants	306:341	membrane-impermeable cryoprotectants	306:341	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29352487	2	5	theme	membrane-impermeable	306:325	arg1	Disaccharides					281:293	Disaccharides	281:293	Disaccharides	281:293	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29352487	1	6	theme	endangered	191:200	arg1	species					202:208	critically endangered species	180:208	critically endangered species	180:208	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	1	6	theme	endangered	191:200	arg1	sinensis					143:150	Acipenser sinensis	133:150	Acipenser sinensis	133:150	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	1	6	theme	endangered	191:200	arg1	dabryanus					166:174	Acipenser dabryanus	156:174	Acipenser dabryanus	156:174	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	8	7	theme	sperm	1205:1209	arg1	quality					1211:1217	sperm quality	1205:1217	sperm quality	1205:1217	Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis.					
29352487	10	8	theme	path	1650:1653	arg1	velocity					1655:1662	average path velocity	1642:1662	average path velocity	1642:1662	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	11	9	theme	expression	1878:1887	arg1	levels					1889:1894	PMCA2 expression levels	1872:1894	PMCA2 expression levels	1872:1894	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	9	theme	expression	1878:1887	arg1	indicator					1922:1930	a valuable indicator	1911:1930	a valuable indicator of sperm quality in A. sinensis	1911:1962	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	9	theme	expression	1878:1887	arg1	ENO3					1863:1866	ENO3	1863:1866	ENO3	1863:1866	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	10	dep	A. sinensis	1811:1821	arg1	sperm					1840:1844	sperm	1840:1844	sperm	1840:1844	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	1	11	theme	germplasm	214:222	arg1	conservation					224:235	germplasm conservation	214:235	germplasm conservation via cryopreservation of sperm	214:265	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	4	12	theme	A. dabryanus	683:694	arg1	sperm					696:700	A. dabryanus sperm	683:700	A. dabryanus sperm	683:700	We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm.					
29352487	6	13	theme	best	933:936	arg1	results					938:944	the best results	929:944	the best results for both A. sinensis and A. dabryanus	929:982	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	7	14	theme	A. sinensis	1053:1063	arg1	sperm					1065:1069	cryopreserved A. sinensis sperm	1039:1069	cryopreserved A. sinensis sperm	1039:1069	Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting.					
29352487	7	15	theme	PMCA2	998:1002	arg1	levels					1015:1020	Enolase3 and PMCA2 expression levels	985:1020	Enolase3 and PMCA2 expression levels	985:1020	Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting.					
29352487	11	16	theme	A. dabryanus	1827:1838	arg1	cryopreservation					1791:1806	cryopreservation	1791:1806	cryopreservation of A. sinensis and A. dabryanus sperm	1791:1844	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	7	17	theme	Enolase3	985:992	arg1	levels					1015:1020	Enolase3 and PMCA2 expression levels	985:1020	Enolase3 and PMCA2 expression levels	985:1020	Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting.					
29352487	10	18	from	expression	1523:1532	arg1	sperm					1703:1707	cryopreserved A. sinensis sperm	1677:1707	cryopreserved A. sinensis sperm	1677:1707	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	11	19	theme	A. sinensis	1811:1821	arg1	cryopreservation					1791:1806	cryopreservation	1791:1806	cryopreservation of A. sinensis and A. dabryanus sperm	1791:1844	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	0	20	theme	plasma	61:66	arg1	ATPase					82:87	plasma membrane Ca2+ ATPase	61:87	plasma membrane Ca2+ ATPase	61:87	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	1	21	theme	Acipenser	156:164	arg1	species					202:208	critically endangered species	180:208	critically endangered species	180:208	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	1	21	theme	Acipenser	156:164	arg1	sinensis					143:150	Acipenser sinensis	133:150	Acipenser sinensis	133:150	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	1	21	theme	Acipenser	156:164	arg1	dabryanus					166:174	Acipenser dabryanus	156:174	Acipenser dabryanus	156:174	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	0	22	theme	Ca2+	77:80	arg1	ATPase					82:87	plasma membrane Ca2+ ATPase	61:87	plasma membrane Ca2+ ATPase	61:87	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	9	23	theme	PMCA2	1313:1317	arg1	ENO3					1304:1307	relative ENO3 and PMCA2 expression levels	1295:1335	ENO3	1304:1307	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	9	23	theme	PMCA2	1313:1317	arg1	levels					1330:1335	relative ENO3 and PMCA2 expression levels	1295:1335	levels	1330:1335	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	9	23	theme	PMCA2	1313:1317	arg1	highest					1346:1352	highest	1346:1352	highest	1346:1352	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	10	24	from	parameters	1553:1562	arg1	sperm					1703:1707	cryopreserved A. sinensis sperm	1677:1707	cryopreserved A. sinensis sperm	1677:1707	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	25	theme	percentage	1474:1483	arg1	integrity					1494:1502	percentage membrane integrity	1474:1502	percentage membrane integrity	1474:1502	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	0	26	theme	isoform	89:95	arg1	combinations					13:24	Disaccharide combinations	0:24	Disaccharide combinations	0:24	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	0	26	theme	isoform	89:95	arg1	expression					34:43	the expression	30:43	the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation	30:130	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	10	27	theme	PMCA2	1517:1521	arg1	expression					1523:1532	PMCA2 expression	1517:1532	PMCA2 expression	1517:1532	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	0	28	theme	sturgeon	100:107	arg1	cryopreservation					115:130	sturgeon sperm cryopreservation	100:130	sturgeon sperm cryopreservation	100:130	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	0	29	from	expression	34:43	arg1	cryopreservation					115:130	sturgeon sperm cryopreservation	100:130	sturgeon sperm cryopreservation	100:130	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	11	30	from	indicator	1922:1930	arg1	A. sinensis					1952:1962	A. sinensis	1952:1962	A. sinensis	1952:1962	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	10	31	theme	A. sinensis	1691:1701	arg1	sperm					1703:1707	cryopreserved A. sinensis sperm	1677:1707	cryopreserved A. sinensis sperm	1677:1707	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	0	32	theme	Disaccharide	0:11	arg1	combinations					13:24	Disaccharide combinations	0:24	Disaccharide combinations	0:24	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	10	33	theme	motility	1544:1551	arg1	percentage					1565:1574	percentage	1565:1574	percentage of motile sperm	1565:1590	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	33	theme	motility	1544:1551	arg1	parameters					1553:1562	sperm motility parameters	1538:1562	sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05)	1538:1672	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	2	34	theme	sperm	441:445	arg1	quality					447:453	sperm quality	441:453	sperm quality	441:453	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29352487	4	35	theme	sucrose	585:591	arg1	use					578:580	use	578:580	use of sucrose or trehalose alone and in combination	578:629	We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm.					
29352487	1	36	dep	species	202:208	arg1	so					211:212	so	211:212	so	211:212	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	9	37	theme	cryopreserved	1368:1380	arg1	sperm					1394:1398	cryopreserved A. sinensis sperm	1368:1398	cryopreserved A. sinensis sperm	1368:1398	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	11	38	theme	disaccharide	1743:1754	arg1	combination					1756:1766	the optimal disaccharide combination	1731:1766	the optimal disaccharide combination	1731:1766	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	3	39	from	characteristics	476:490	arg1	stock					527:531	cultured brood stock	512:531	cultured brood stock	512:531	We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus.					
29352487	3	40	theme	cultured	512:519	arg1	stock					527:531	cultured brood stock	512:531	cultured brood stock	512:531	We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus.					
29352487	0	41	theme	enolase3	48:55	arg1	isoform					89:95	enolase3 and plasma membrane Ca2+ ATPase isoform	48:95	enolase3 and plasma membrane Ca2+ ATPase isoform	48:95	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	8	42	theme	PMCA2	1111:1115	arg1	levels					1128:1133	Relative ENO3 and PMCA2 expression levels	1093:1133	levels	1128:1133	Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis.					
29352487	11	43	theme	quality	1941:1947	arg1	levels					1889:1894	PMCA2 expression levels	1872:1894	PMCA2 expression levels	1872:1894	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	43	theme	quality	1941:1947	arg1	indicator					1922:1930	a valuable indicator	1911:1930	a valuable indicator of sperm quality in A. sinensis	1911:1962	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	43	theme	quality	1941:1947	arg1	ENO3					1863:1866	ENO3	1863:1866	ENO3	1863:1866	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	3	44	theme	quality	501:507	arg1	characteristics					476:490	seven characteristics	470:490	seven characteristics of sperm quality in cultured brood stock	470:531	We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus.					
29352487	10	45	theme	curvilinear	1593:1603	arg1	velocity					1605:1612	curvilinear velocity	1593:1612	curvilinear velocity	1593:1612	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	1	46	theme	sperm	261:265	arg1	conservation					224:235	germplasm conservation	214:235	germplasm conservation via cryopreservation of sperm	214:265	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	4	47	theme	different	634:642	arg1	concentrations					644:657	different concentrations	634:657	different concentrations for cryopreservation of A. dabryanus sperm	634:700	We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm.					
29352487	10	48	theme	sperm	1586:1590	arg1	percentage					1565:1574	percentage	1565:1574	percentage of motile sperm	1565:1590	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	48	theme	sperm	1586:1590	arg1	parameters					1553:1562	sperm motility parameters	1538:1562	sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05)	1538:1672	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	2	49	theme	Ca2+	384:387	arg1	ATPase					389:394	plasma membrane Ca2+ ATPase	368:394	plasma membrane Ca2+ ATPase isoform (PMCA2)	368:410	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29352487	6	50	theme	L15	914:916	arg1	trehalose					903:911	trehalose	903:911	trehalose (L15 T15 )	903:922	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	6	50	theme	L15	914:916	arg1	T15					918:920	L15 T15	914:920	L15 T15	914:920	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	4	51	theme	trehalose	596:604	arg1	use					578:580	use	578:580	use of sucrose or trehalose alone and in combination	578:629	We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm.					
29352487	10	52	theme	significant	1412:1422	arg1	correlations					1433:1444	significant positive correlations	1412:1444	significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm	1412:1707	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	6	53	theme	trehalose	903:911	arg1	mixture					880:886	a mixture	878:886	a mixture of lactose and trehalose (L15 T15 )	878:922	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	2	54	theme	plasma	368:373	arg1	ATPase					389:394	plasma membrane Ca2+ ATPase	368:394	plasma membrane Ca2+ ATPase isoform (PMCA2)	368:410	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29352487	8	55	theme	disaccharide	1179:1190	arg1	composition					1192:1202	disaccharide composition	1179:1202	disaccharide composition	1179:1202	Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis.					
29352487	6	56	theme	lactose	891:897	arg1	mixture					880:886	a mixture	878:886	a mixture of lactose and trehalose (L15 T15 )	878:922	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	4	57	theme	sperm	696:700	arg1	cryopreservation					663:678	cryopreservation	663:678	cryopreservation of A. dabryanus sperm	663:700	We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A. dabryanus sperm.					
29352487	10	58	dep	percentage	1565:1574	arg1	p < .05					1665:1671	p < .05	1665:1671	p < .05	1665:1671	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	58	dep	percentage	1565:1574	arg1	velocity					1655:1662	average path velocity	1642:1662	average path velocity	1642:1662	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	58	dep	percentage	1565:1574	arg1	velocity					1605:1612	curvilinear velocity	1593:1612	curvilinear velocity	1593:1612	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	58	dep	percentage	1565:1574	arg1	velocity					1629:1636	straight-line velocity	1615:1636	straight-line velocity	1615:1636	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	59	theme	ENO3	1454:1457	arg1	expression					1459:1468	ENO3 expression	1454:1468	ENO3 expression	1454:1468	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	60	theme	average	1642:1648	arg1	velocity					1655:1662	average path velocity	1642:1662	average path velocity	1642:1662	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	5	61	theme	trehalose	739:747	arg1	optimal					764:770	optimal	764:770	optimal	764:770	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	5	61	theme	trehalose	739:747	arg1	concentration					709:721	A low concentration	703:721	A low concentration of sucrose plus trehalose (S15 T15 )	703:758	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	5	61	theme	trehalose	739:747	arg1	T15					754:756	S15 T15	750:756	S15 T15	750:756	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	11	62	theme	PMCA2	1872:1876	arg1	levels					1889:1894	PMCA2 expression levels	1872:1894	PMCA2 expression levels	1872:1894	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	62	theme	PMCA2	1872:1876	arg1	indicator					1922:1930	a valuable indicator	1911:1930	a valuable indicator of sperm quality in A. sinensis	1911:1962	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	62	theme	PMCA2	1872:1876	arg1	ENO3					1863:1866	ENO3	1863:1866	ENO3	1863:1866	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	5	63	theme	S15	750:752	arg1	concentration					709:721	A low concentration	703:721	A low concentration of sucrose plus trehalose (S15 T15 )	703:758	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	5	63	theme	S15	750:752	arg1	T15					754:756	S15 T15	750:756	S15 T15	750:756	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	8	64	theme	protein	1223:1229	arg1	expression					1231:1240	protein expression	1223:1240	protein expression	1223:1240	Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis.					
29352487	7	65	theme	cryopreserved	1039:1051	arg1	sperm					1065:1069	cryopreserved A. sinensis sperm	1039:1069	cryopreserved A. sinensis sperm	1039:1069	Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting.					
29352487	7	66	theme	Western	1075:1081	arg1	blotting					1083:1090	Western blotting	1075:1090	Western blotting	1075:1090	Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting.					
29352487	5	67	theme	sucrose	726:732	arg1	optimal					764:770	optimal	764:770	optimal	764:770	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	5	67	theme	sucrose	726:732	arg1	concentration					709:721	A low concentration	703:721	A low concentration of sucrose plus trehalose (S15 T15 )	703:758	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	5	67	theme	sucrose	726:732	arg1	T15					754:756	S15 T15	750:756	S15 T15	750:756	A low concentration of sucrose plus trehalose (S15 T15 ) was optimal.					
29352487	7	68	theme	expression	1004:1013	arg1	levels					1015:1020	Enolase3 and PMCA2 expression levels	985:1020	Enolase3 and PMCA2 expression levels	985:1020	Enolase3 and PMCA2 expression levels were measured in cryopreserved A. sinensis sperm via Western blotting.					
29352487	1	69	theme	Acipenser	133:141	arg1	species					202:208	critically endangered species	180:208	critically endangered species	180:208	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	1	69	theme	Acipenser	133:141	arg1	sinensis					143:150	Acipenser sinensis	133:150	Acipenser sinensis	133:150	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	1	69	theme	Acipenser	133:141	arg1	dabryanus					166:174	Acipenser dabryanus	156:174	Acipenser dabryanus	156:174	Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary.					
29352487	11	70	theme	valuable	1913:1920	arg1	levels					1889:1894	PMCA2 expression levels	1872:1894	PMCA2 expression levels	1872:1894	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	70	theme	valuable	1913:1920	arg1	indicator					1922:1930	a valuable indicator	1911:1930	a valuable indicator of sperm quality in A. sinensis	1911:1962	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	70	theme	valuable	1913:1920	arg1	ENO3					1863:1866	ENO3	1863:1866	ENO3	1863:1866	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	0	71	theme	membrane	68:75	arg1	ATPase					82:87	plasma membrane Ca2+ ATPase	61:87	plasma membrane Ca2+ ATPase	61:87	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	9	72	theme	relative	1295:1302	arg1	ENO3					1304:1307	relative ENO3 and PMCA2 expression levels	1295:1335	ENO3	1304:1307	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	9	72	theme	relative	1295:1302	arg1	levels					1330:1335	relative ENO3 and PMCA2 expression levels	1295:1335	levels	1330:1335	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	9	72	theme	relative	1295:1302	arg1	highest					1346:1352	highest	1346:1352	highest	1346:1352	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	0	73	theme	ATPase	82:87	arg1	isoform					89:95	enolase3 and plasma membrane Ca2+ ATPase isoform	48:95	enolase3 and plasma membrane Ca2+ ATPase isoform	48:95	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	0	74	from	combinations	13:24	arg1	cryopreservation					115:130	sturgeon sperm cryopreservation	100:130	sturgeon sperm cryopreservation	100:130	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	10	75	theme	membrane	1485:1492	arg1	integrity					1494:1502	percentage membrane integrity	1474:1502	percentage membrane integrity	1474:1502	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	11	76	from	quality	1941:1947	arg1	A. sinensis					1952:1962	A. sinensis	1952:1962	A. sinensis	1952:1962	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	9	77	theme	expression	1319:1328	arg1	ENO3					1304:1307	relative ENO3 and PMCA2 expression levels	1295:1335	ENO3	1304:1307	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	9	77	theme	expression	1319:1328	arg1	levels					1330:1335	relative ENO3 and PMCA2 expression levels	1295:1335	levels	1330:1335	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	9	77	theme	expression	1319:1328	arg1	highest					1346:1352	highest	1346:1352	highest	1346:1352	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	11	78	from	A. sinensis	1952:1962	arg1	levels					1889:1894	PMCA2 expression levels	1872:1894	PMCA2 expression levels	1872:1894	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	78	from	A. sinensis	1952:1962	arg1	indicator					1922:1930	a valuable indicator	1911:1930	a valuable indicator of sperm quality in A. sinensis	1911:1962	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	11	78	from	A. sinensis	1952:1962	arg1	ENO3					1863:1866	ENO3	1863:1866	ENO3	1863:1866	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	0	79	theme	sperm	109:113	arg1	cryopreservation					115:130	sturgeon sperm cryopreservation	100:130	sturgeon sperm cryopreservation	100:130	Disaccharide combinations and the expression of enolase3 and plasma membrane Ca2+ ATPase isoform in sturgeon sperm cryopreservation.					
29352487	10	80	theme	cryopreserved	1677:1689	arg1	sperm					1703:1707	cryopreserved A. sinensis sperm	1677:1707	cryopreserved A. sinensis sperm	1677:1707	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	9	81	theme	L15	1357:1359	arg1	T15					1361:1363	L15 T15	1357:1363	L15 T15	1357:1363	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	8	82	theme	Relative	1093:1100	arg1	ENO3					1102:1105	Relative ENO3 and PMCA2 expression levels	1093:1133	ENO3	1102:1105	Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis.					
29352487	10	83	theme	sperm	1538:1542	arg1	percentage					1565:1574	percentage	1565:1574	percentage of motile sperm	1565:1590	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	10	83	theme	sperm	1538:1542	arg1	parameters					1553:1562	sperm motility parameters	1538:1562	sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05)	1538:1672	There were significant positive correlations between ENO3 expression and percentage membrane integrity, and between PMCA2 expression and sperm motility parameters (percentage of motile sperm, curvilinear velocity, straight-line velocity and average path velocity; p < .05) in cryopreserved A. sinensis sperm.					
29352487	3	84	theme	sperm	495:499	arg1	quality					501:507	sperm quality	495:507	sperm quality	495:507	We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus.					
29352487	11	85	theme	optimal	1735:1741	arg1	combination					1756:1766	the optimal disaccharide combination	1731:1766	the optimal disaccharide combination	1731:1766	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	6	86	theme	extender	787:794	arg1	Mixing					773:778	Mixing	773:778	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures	773:862	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	9	87	theme	A. sinensis	1382:1392	arg1	sperm					1394:1398	cryopreserved A. sinensis sperm	1368:1398	cryopreserved A. sinensis sperm	1368:1398	The results showed that relative ENO3 and PMCA2 expression levels were the highest at L15 T15 in cryopreserved A. sinensis sperm.					
29352487	8	88	theme	expression	1117:1126	arg1	levels					1128:1133	Relative ENO3 and PMCA2 expression levels	1093:1133	levels	1128:1133	Relative ENO3 and PMCA2 expression levels were examined, and the relationship between disaccharide composition, sperm quality and protein expression was explored in A. sinensis.					
29352487	3	89	theme	brood	521:525	arg1	stock					527:531	cultured brood stock	512:531	cultured brood stock	512:531	We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus.					
29352487	11	90	theme	sperm	1935:1939	arg1	quality					1941:1947	sperm quality	1935:1947	sperm quality in A. sinensis	1935:1962	Our results indicate the optimal disaccharide combination and concentrations for cryopreservation of A. sinensis and A. dabryanus sperm and suggest that ENO3 and PMCA2 expression levels could serve as a valuable indicator of sperm quality in A. sinensis.					
29352487	6	91	theme	pairwise	846:853	arg1	mixtures					855:862	pairwise mixtures	846:862	pairwise mixtures	846:862	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	6	92	with	Mixing	773:778	arg1	lactose					810:816	lactose	810:816	lactose	810:816	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	6	92	with	Mixing	773:778	arg1	trehalose					822:830	trehalose	822:830	trehalose	822:830	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	6	92	with	Mixing	773:778	arg1	sucrose					801:807	sucrose	801:807	sucrose	801:807	Mixing of the extender with sucrose, lactose, or trehalose alone or with pairwise mixtures revealed that a mixture of lactose and trehalose (L15 T15 ) gave the best results for both A. sinensis and A. dabryanus.					
29352487	2	93	theme	ATPase	389:394	arg1	enolase3					348:355	enolase3	348:355	enolase3 (ENO3)	348:362	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29352487	2	93	theme	ATPase	389:394	arg1	PMCA2					405:409	PMCA2	405:409	PMCA2	405:409	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29352487	2	93	theme	ATPase	389:394	arg1	proteins					416:423	proteins	416:423	proteins associated with sperm quality	416:453	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29352487	2	93	theme	ATPase	389:394	arg1	isoform					396:402	plasma membrane Ca2+ ATPase isoform	368:402	plasma membrane Ca2+ ATPase isoform (PMCA2)	368:410	Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality.					
29857273	7	0	theme	endosome	1313:1320	arg1	escape					1322:1327	early endosome escape	1307:1327	early endosome escape	1307:1327	We introduced pH sensitive moieties within the construct to enhance early endosome escape and efficiently improve the silencing effect in vitro.					
29857273	1	1	theme	constructs	248:257	arg1	ability					233:239	the ability	229:239	the ability of the constructs to permeate the 3D multicellular matrix	229:297	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	1	2	theme	constructs	128:137	arg1	penetration					95:105	The poor penetration	86:105	The poor penetration of nanocarrier-siRNA constructs into tumor tissue	86:155	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	1	2	theme	constructs	128:137	arg1	hurdle					168:173	a major hurdle	160:173	a major hurdle	160:173	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	6	3	theme	cells	1232:1236	arg1	penetration					1129:1139	penetration	1129:1139	penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells	1129:1236	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	9	4	theme	circulation	1560:1570	arg1	lifetime					1572:1579	an in vivo circulation lifetime	1549:1579	an in vivo circulation lifetime of 8 h in mice	1549:1594	The optimized construct exhibited an in vivo circulation lifetime of 8 h in mice, with preferential accumulation in the liver.					
29857273	6	5	theme	prostate	1216:1223	arg1	cells					1232:1236	human prostate cancer cells	1210:1236	human prostate cancer cells	1210:1236	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	8	6	theme	multicellular	1453:1465	arg1	spheroids					1467:1475	3D multicellular spheroids	1450:1475	3D multicellular spheroids causing ∼60% specific gene silencing	1450:1512	Glycogen-siRNA constructs were found to mediate gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing.					
29857273	3	7	theme	different	595:603	arg1	bio-sources					605:615	different bio-sources	595:615	different bio-sources	595:615	Glycogen nanoparticles from different bio-sources and with different structural features were investigated.					
29857273	5	8	theme	20 nm	880:884	arg1	nanoparticles					895:907	20 nm glycogen nanoparticles	880:907	20 nm glycogen nanoparticles	880:907	Our studies suggest that 20 nm glycogen nanoparticles are optimal for complexation and efficient delivery of siRNA.					
29857273	4	9	theme	glycogen	695:702	arg1	systems					758:764	suboptimal systems	747:764	suboptimal systems for complexation of nucleic acids	747:798	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	4	9	theme	glycogen	695:702	arg1	nanoparticles					704:716	larger glycogen nanoparticles	688:716	larger glycogen nanoparticles ranging from 50 to 80 nm	688:741	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	6	10	theme	surface	997:1003	arg1	charge					1005:1010	surface charge	997:1010	surface charge	997:1010	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	6	11	with	interactions	1086:1097	arg1	proteins					1110:1117	serum proteins	1104:1117	serum proteins	1104:1117	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	11	12	theme	therapeutic	1887:1897	arg1	efficacy					1899:1906	the predictive therapeutic efficacy	1872:1906	the predictive therapeutic efficacy of glycogen-based platforms	1872:1934	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	3	13	theme	different	626:634	arg1	features					647:654	different structural features	626:654	different structural features	626:654	Glycogen nanoparticles from different bio-sources and with different structural features were investigated.					
29857273	8	14	theme	∼60	1485:1487	arg1	%					1488:1488	%	1488:1488	%	1488:1488	Glycogen-siRNA constructs were found to mediate gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing.					
29857273	11	15	theme	3D	1819:1820	arg1	models					1846:1851	3D cultured prostate tumor models	1819:1851	3D cultured prostate tumor models	1819:1851	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	9	16	theme	preferential	1602:1613	arg1	accumulation					1615:1626	preferential accumulation	1602:1626	preferential accumulation in the liver	1602:1639	The optimized construct exhibited an in vivo circulation lifetime of 8 h in mice, with preferential accumulation in the liver.					
29857273	7	17	theme	early	1307:1311	arg1	escape					1322:1327	early endosome escape	1307:1327	early endosome escape	1307:1327	We introduced pH sensitive moieties within the construct to enhance early endosome escape and efficiently improve the silencing effect in vitro.					
29857273	1	18	theme	physicochemical	322:336	arg1	properties					338:347	their physicochemical properties	316:347	their physicochemical properties	316:347	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	5	19	theme	efficient	942:950	arg1	delivery					952:959	efficient delivery	942:959	efficient delivery	942:959	Our studies suggest that 20 nm glycogen nanoparticles are optimal for complexation and efficient delivery of siRNA.					
29857273	0	20	from	constructs	22:31	arg1	spheroids					75:83	multicellular tumor spheroids	55:83	multicellular tumor spheroids	55:83	Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids.					
29857273	4	21	theme	suboptimal	747:756	arg1	nanoparticles					704:716	larger glycogen nanoparticles	688:716	larger glycogen nanoparticles ranging from 50 to 80 nm	688:741	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	4	21	theme	suboptimal	747:756	arg1	systems					758:764	suboptimal systems	747:764	suboptimal systems for complexation of nucleic acids	747:798	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	2	22	theme	silencing	549:557	arg1	effect					559:564	a significant gene silencing effect	530:564	a significant gene silencing effect	530:564	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
29857273	11	23	theme	prostate	1831:1838	arg1	models					1846:1851	3D cultured prostate tumor models	1819:1851	3D cultured prostate tumor models	1819:1851	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	6	24	theme	glycogen-siRNA	1025:1038	arg1	constructs					1040:1049	glycogen-siRNA constructs	1025:1049	glycogen-siRNA constructs	1025:1049	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	2	25	theme	significant	532:542	arg1	effect					559:564	a significant gene silencing effect	530:564	a significant gene silencing effect	530:564	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
29857273	4	26	theme	size	823:826	arg1	control					808:814	fine control	803:814	fine control of the size of constructs	803:840	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	2	27	theme	glycogen	387:394	arg1	nanoparticles					396:408	soft glycogen nanoparticles	382:408	soft glycogen nanoparticles	382:408	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
29857273	4	28	theme	acids	794:798	arg1	complexation					770:781	complexation	770:781	complexation of nucleic acids	770:798	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	0	29	theme	Glycogen-nucleic	0:15	arg1	constructs					22:31	Glycogen-nucleic acid constructs	0:31	Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids	0:83	Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids.					
29857273	2	30	theme	glycogen-siRNA	433:446	arg1	constructs					448:457	glycogen-siRNA constructs	433:457	glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect	433:564	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
29857273	4	31	theme	fine	803:806	arg1	control					808:814	fine control	803:814	fine control of the size of constructs	803:840	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	1	32	theme	multicellular	278:290	arg1	matrix					292:297	the 3D multicellular matrix	271:297	the 3D multicellular matrix	271:297	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	1	33	theme	nanocarrier-siRNA	110:126	arg1	constructs					128:137	nanocarrier-siRNA constructs	110:137	nanocarrier-siRNA constructs	110:137	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	2	34	theme	multicellular	490:502	arg1	spheroids					510:518	multicellular tumor spheroids	490:518	multicellular tumor spheroids	490:518	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
29857273	8	35	theme	specific	1490:1497	arg1	silencing					1504:1512	∼60% specific gene silencing	1485:1512	∼60% specific gene silencing	1485:1512	Glycogen-siRNA constructs were found to mediate gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing.					
29857273	0	36	theme	gene	37:40	arg1	silencing					42:50	gene silencing	37:50	gene silencing	37:50	Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids.					
29857273	0	37	theme	tumor	69:73	arg1	spheroids					75:83	multicellular tumor spheroids	55:83	multicellular tumor spheroids	55:83	Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids.					
29857273	11	38	theme	human	1939:1943	arg1	conditions					1959:1968	human physiological conditions	1939:1968	human physiological conditions	1939:1968	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	3	39	with	nanoparticles	576:588	arg1	features					647:654	different structural features	626:654	different structural features	626:654	Glycogen nanoparticles from different bio-sources and with different structural features were investigated.					
29857273	10	40	from	kidney	1665:1670	arg1	mice					1727:1730	mice	1727:1730	mice	1727:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	9	41	from	lifetime	1572:1579	arg1	mice					1591:1594	mice	1591:1594	mice	1591:1594	The optimized construct exhibited an in vivo circulation lifetime of 8 h in mice, with preferential accumulation in the liver.					
29857273	7	42	theme	effect	1367:1372	arg1	in vitro					1374:1381	the silencing effect in vitro	1353:1381	the silencing effect in vitro	1353:1381	We introduced pH sensitive moieties within the construct to enhance early endosome escape and efficiently improve the silencing effect in vitro.					
29857273	11	43	theme	siRNA	1797:1801	arg1	nanocarriers					1803:1814	siRNA nanocarriers	1797:1814	siRNA nanocarriers	1797:1814	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	4	44	theme	constructs	831:840	arg1	size					823:826	the size	819:826	the size of constructs	819:840	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	11	45	theme	platforms	1926:1934	arg1	efficacy					1899:1906	the predictive therapeutic efficacy	1872:1906	the predictive therapeutic efficacy of glycogen-based platforms	1872:1934	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	10	46	from	lung	1673:1676	arg1	mice					1727:1730	mice	1727:1730	mice	1727:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	6	47	theme	kidney	1182:1187	arg1	cells					1200:1204	human kidney epithelial cells	1176:1204	human kidney epithelial cells	1176:1204	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	6	48	theme	chemical	975:982	arg1	composition					984:994	The chemical composition	971:994	The chemical composition	971:994	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	10	49	from	heart	1687:1691	arg1	mice					1727:1730	mice	1727:1730	mice	1727:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	6	50	theme	multicellular	1149:1161	arg1	spheroids					1163:1171	3D multicellular spheroids	1146:1171	3D multicellular spheroids	1146:1171	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	3	51	theme	Glycogen	567:574	arg1	nanoparticles					576:588	Glycogen nanoparticles	567:588	Glycogen nanoparticles from different bio-sources and with different structural features	567:654	Glycogen nanoparticles from different bio-sources and with different structural features were investigated.					
29857273	9	52	theme	optimized	1519:1527	arg1	construct					1529:1537	The optimized construct	1515:1537	The optimized construct	1515:1537	The optimized construct exhibited an in vivo circulation lifetime of 8 h in mice, with preferential accumulation in the liver.					
29857273	10	53	from	brain	1696:1700	arg1	mice					1727:1730	mice	1727:1730	mice	1727:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	9	54	from	accumulation	1615:1626	arg1	liver					1635:1639	the liver	1631:1639	the liver	1631:1639	The optimized construct exhibited an in vivo circulation lifetime of 8 h in mice, with preferential accumulation in the liver.					
29857273	1	55	theme	in vivo	183:189	arg1	efficacy					191:198	the in vivo efficacy	179:198	the in vivo efficacy of siRNA therapeutics	179:220	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	9	56	theme	in vivo	1552:1558	arg1	lifetime					1572:1579	an in vivo circulation lifetime	1549:1579	an in vivo circulation lifetime of 8 h in mice	1549:1594	The optimized construct exhibited an in vivo circulation lifetime of 8 h in mice, with preferential accumulation in the liver.					
29857273	6	57	theme	cancer	1225:1230	arg1	cells					1232:1236	human prostate cancer cells	1210:1236	human prostate cancer cells	1210:1236	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	11	58	theme	predictive	1876:1885	arg1	efficacy					1899:1906	the predictive therapeutic efficacy	1872:1906	the predictive therapeutic efficacy of glycogen-based platforms	1872:1934	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	10	59	dep	kidney	1665:1670	arg1	the					1661:1663	the	1661:1663	the	1661:1663	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	6	60	theme	human	1210:1214	arg1	cells					1232:1236	human prostate cancer cells	1210:1236	human prostate cancer cells	1210:1236	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	6	61	theme	cells	1200:1204	arg1	penetration					1129:1139	penetration	1129:1139	penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells	1129:1236	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	3	62	theme	structural	636:645	arg1	features					647:654	different structural features	626:654	different structural features	626:654	Glycogen nanoparticles from different bio-sources and with different structural features were investigated.					
29857273	5	63	theme	glycogen	886:893	arg1	nanoparticles					895:907	20 nm glycogen nanoparticles	880:907	20 nm glycogen nanoparticles	880:907	Our studies suggest that 20 nm glycogen nanoparticles are optimal for complexation and efficient delivery of siRNA.					
29857273	4	64	theme	larger	688:693	arg1	systems					758:764	suboptimal systems	747:764	suboptimal systems for complexation of nucleic acids	747:798	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	4	64	theme	larger	688:693	arg1	nanoparticles					704:716	larger glycogen nanoparticles	688:716	larger glycogen nanoparticles ranging from 50 to 80 nm	688:741	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	11	65	theme	glycogen-based	1911:1924	arg1	platforms					1926:1934	glycogen-based platforms	1911:1934	glycogen-based platforms	1911:1934	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	5	66	theme	siRNA	964:968	arg1	delivery					952:959	efficient delivery	942:959	efficient delivery	942:959	Our studies suggest that 20 nm glycogen nanoparticles are optimal for complexation and efficient delivery of siRNA.					
29857273	5	66	theme	siRNA	964:968	arg1	complexation					925:936	complexation	925:936	complexation	925:936	Our studies suggest that 20 nm glycogen nanoparticles are optimal for complexation and efficient delivery of siRNA.					
29857273	1	67	theme	tumor	144:148	arg1	tissue					150:155	tumor tissue	144:155	tumor tissue	144:155	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	8	68	theme	Glycogen-siRNA	1384:1397	arg1	constructs					1399:1408	Glycogen-siRNA constructs	1384:1408	Glycogen-siRNA constructs	1384:1408	Glycogen-siRNA constructs were found to mediate gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing.					
29857273	3	69	from	bio-sources	605:615	arg1	nanoparticles					576:588	Glycogen nanoparticles	567:588	Glycogen nanoparticles from different bio-sources and with different structural features	567:654	Glycogen nanoparticles from different bio-sources and with different structural features were investigated.					
29857273	11	70	theme	cultured	1822:1829	arg1	models					1846:1851	3D cultured prostate tumor models	1819:1851	3D cultured prostate tumor models	1819:1851	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	1	71	theme	major	162:166	arg1	penetration					95:105	The poor penetration	86:105	The poor penetration of nanocarrier-siRNA constructs into tumor tissue	86:155	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	1	71	theme	major	162:166	arg1	hurdle					168:173	a major hurdle	160:173	a major hurdle	160:173	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	2	72	theme	tumor	504:508	arg1	spheroids					510:518	multicellular tumor spheroids	490:518	multicellular tumor spheroids	490:518	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
29857273	6	73	theme	constructs	1040:1049	arg1	composition					984:994	The chemical composition	971:994	The chemical composition	971:994	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	6	73	theme	constructs	1040:1049	arg1	charge					1005:1010	surface charge	997:1010	surface charge	997:1010	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	6	73	theme	constructs	1040:1049	arg1	size					1017:1020	size	1017:1020	size	1017:1020	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	2	74	theme	gene	544:547	arg1	effect					559:564	a significant gene silencing effect	530:564	a significant gene silencing effect	530:564	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
29857273	8	75	theme	gene	1432:1435	arg1	silencing					1437:1445	gene silencing	1432:1445	gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing	1432:1512	Glycogen-siRNA constructs were found to mediate gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing.					
29857273	11	76	theme	tumor	1840:1844	arg1	models					1846:1851	3D cultured prostate tumor models	1819:1851	3D cultured prostate tumor models	1819:1851	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	7	77	theme	sensitive	1256:1264	arg1	moieties					1266:1273	pH sensitive moieties	1253:1273	pH sensitive moieties	1253:1273	We introduced pH sensitive moieties within the construct to enhance early endosome escape and efficiently improve the silencing effect in vitro.					
29857273	1	78	theme	siRNA	203:207	arg1	therapeutics					209:220	siRNA therapeutics	203:220	siRNA therapeutics	203:220	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	6	79	theme	3D	1146:1147	arg1	spheroids					1163:1171	3D multicellular spheroids	1146:1171	3D multicellular spheroids	1146:1171	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	10	80	theme	toxicity	1715:1722	arg1	signs					1706:1710	signs	1706:1710	signs of toxicity in mice	1706:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	80	theme	toxicity	1715:1722	arg1	heart					1687:1691	heart	1687:1691	heart	1687:1691	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	80	theme	toxicity	1715:1722	arg1	kidney					1665:1670	kidney	1665:1670	kidney	1665:1670	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	80	theme	toxicity	1715:1722	arg1	lung					1673:1676	lung	1673:1676	lung	1673:1676	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	80	theme	toxicity	1715:1722	arg1	brain					1696:1700	brain	1696:1700	brain	1696:1700	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	80	theme	toxicity	1715:1722	arg1	spleen					1679:1684	spleen	1679:1684	spleen	1679:1684	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	0	81	theme	acid	17:20	arg1	constructs					22:31	Glycogen-nucleic acid constructs	0:31	Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids	0:83	Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids.					
29857273	1	82	theme	poor	90:93	arg1	penetration					95:105	The poor penetration	86:105	The poor penetration of nanocarrier-siRNA constructs into tumor tissue	86:155	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	1	82	theme	poor	90:93	arg1	hurdle					168:173	a major hurdle	160:173	a major hurdle	160:173	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	2	83	theme	constructs	448:457	arg1	engineering					418:428	the engineering	414:428	the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect	414:564	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
29857273	4	84	theme	nucleic	786:792	arg1	acids					794:798	nucleic acids	786:798	nucleic acids	786:798	We show that larger glycogen nanoparticles ranging from 50 to 80 nm are suboptimal systems for complexation of nucleic acids if fine control of the size of constructs is required.					
29857273	1	85	theme	3D	275:276	arg1	matrix					292:297	the 3D multicellular matrix	271:297	the 3D multicellular matrix	271:297	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	8	86	theme	3D	1450:1451	arg1	spheroids					1467:1475	3D multicellular spheroids	1450:1475	3D multicellular spheroids causing ∼60% specific gene silencing	1450:1512	Glycogen-siRNA constructs were found to mediate gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing.					
29857273	1	87	theme	therapeutics	209:220	arg1	efficacy					191:198	the in vivo efficacy	179:198	the in vivo efficacy of siRNA therapeutics	179:220	The poor penetration of nanocarrier-siRNA constructs into tumor tissue is a major hurdle for the in vivo efficacy of siRNA therapeutics, where the ability of the constructs to permeate the 3D multicellular matrix is determined by their physicochemical properties.					
29857273	6	88	theme	serum	1104:1108	arg1	proteins					1110:1117	serum proteins	1104:1117	serum proteins	1104:1117	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	8	89	theme	%	1488:1488	arg1	silencing					1504:1512	∼60% specific gene silencing	1485:1512	∼60% specific gene silencing	1485:1512	Glycogen-siRNA constructs were found to mediate gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing.					
29857273	2	90	theme	nanoparticles	396:408	arg1	use					375:377	the use	371:377	the use of soft glycogen nanoparticles	371:408	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
29857273	0	91	theme	multicellular	55:67	arg1	spheroids					75:83	multicellular tumor spheroids	55:83	multicellular tumor spheroids	55:83	Glycogen-nucleic acid constructs for gene silencing in multicellular tumor spheroids.					
29857273	10	92	from	accumulation	1645:1656	arg1	kidney					1665:1670	kidney	1665:1670	kidney	1665:1670	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	92	from	accumulation	1645:1656	arg1	signs					1706:1710	signs	1706:1710	signs of toxicity in mice	1706:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	92	from	accumulation	1645:1656	arg1	heart					1687:1691	heart	1687:1691	heart	1687:1691	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	92	from	accumulation	1645:1656	arg1	spleen					1679:1684	spleen	1679:1684	spleen	1679:1684	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	92	from	accumulation	1645:1656	arg1	lung					1673:1676	lung	1673:1676	lung	1673:1676	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	9	93	theme	8 h	1584:1586	arg1	lifetime					1572:1579	an in vivo circulation lifetime	1549:1579	an in vivo circulation lifetime of 8 h in mice	1549:1594	The optimized construct exhibited an in vivo circulation lifetime of 8 h in mice, with preferential accumulation in the liver.					
29857273	10	94	from	signs	1706:1710	arg1	mice					1727:1730	mice	1727:1730	mice	1727:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	7	95	theme	silencing	1357:1365	arg1	in vitro					1374:1381	the silencing effect in vitro	1353:1381	the silencing effect in vitro	1353:1381	We introduced pH sensitive moieties within the construct to enhance early endosome escape and efficiently improve the silencing effect in vitro.					
29857273	10	96	from	spleen	1679:1684	arg1	mice					1727:1730	mice	1727:1730	mice	1727:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	8	97	theme	gene	1499:1502	arg1	silencing					1504:1512	∼60% specific gene silencing	1485:1512	∼60% specific gene silencing	1485:1512	Glycogen-siRNA constructs were found to mediate gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing.					
29857273	11	98	theme	physiological	1945:1957	arg1	conditions					1959:1968	human physiological conditions	1939:1968	human physiological conditions	1939:1968	Our results highlight the potential for screening siRNA nanocarriers in 3D cultured prostate tumor models, thereby improving the predictive therapeutic efficacy of glycogen-based platforms in human physiological conditions.					
29857273	10	99	from	toxicity	1715:1722	arg1	mice					1727:1730	mice	1727:1730	mice	1727:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	6	100	theme	epithelial	1189:1198	arg1	cells					1200:1204	human kidney epithelial cells	1176:1204	human kidney epithelial cells	1176:1204	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	8	101	from	silencing	1437:1445	arg1	spheroids					1467:1475	3D multicellular spheroids	1450:1475	3D multicellular spheroids causing ∼60% specific gene silencing	1450:1512	Glycogen-siRNA constructs were found to mediate gene silencing in 3D multicellular spheroids causing ∼60% specific gene silencing.					
29857273	10	102	from	mice	1727:1730	arg1	signs					1706:1710	signs	1706:1710	signs of toxicity in mice	1706:1730	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	102	from	mice	1727:1730	arg1	heart					1687:1691	heart	1687:1691	heart	1687:1691	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	102	from	mice	1727:1730	arg1	kidney					1665:1670	kidney	1665:1670	kidney	1665:1670	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	102	from	mice	1727:1730	arg1	lung					1673:1676	lung	1673:1676	lung	1673:1676	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	102	from	mice	1727:1730	arg1	brain					1696:1700	brain	1696:1700	brain	1696:1700	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	10	102	from	mice	1727:1730	arg1	spleen					1679:1684	spleen	1679:1684	spleen	1679:1684	No accumulation in the kidney, lung, spleen, heart or brain, or signs of toxicity in mice were observed.					
29857273	6	103	theme	human	1176:1180	arg1	cells					1200:1204	human kidney epithelial cells	1176:1204	human kidney epithelial cells	1176:1204	The chemical composition, surface charge, and size of glycogen-siRNA constructs were finely controlled to minimize interactions with serum proteins and allow penetration into 3D multicellular spheroids of human kidney epithelial cells and human prostate cancer cells.					
29857273	2	104	theme	soft	382:385	arg1	nanoparticles					396:408	soft glycogen nanoparticles	382:408	soft glycogen nanoparticles	382:408	Herein, we optimized the use of soft glycogen nanoparticles for the engineering of glycogen-siRNA constructs that can efficiently penetrate multicellular tumor spheroids and exert a significant gene silencing effect.					
31638820	2	0	theme	biomimetic	350:359	arg1	materials					361:369	functional biomimetic materials	339:369	functional biomimetic materials	339:369	Synthetic biomolecular liquids offer a means to study the mechanisms of this process, as well as offering a route to the creation of functional biomimetic materials.					
31638820	0	1	theme	Liquid	71:76	arg1	DNA					67:69	a DNA Liquid	65:76	a DNA Liquid	65:76	Length-Dependence and Spatial Structure of DNA Partitioning into a DNA Liquid.					
31638820	3	2	theme	small	493:497	arg1	"					524:524	"nanostars"	514:524	"nanostars"	514:524	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	3	2	theme	small	493:497	arg1	particles					503:511	small DNA particles	493:511	small DNA particles ("nanostars") whose phase separation is driven by base pairing	493:574	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	6	3	theme	droplets	1084:1091	arg1	surface					1066:1072	the surface	1062:1072	the surface of liquid droplets	1062:1091	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	3	4	theme	nanostars	515:523	arg1	"					524:524	"nanostars"	514:524	"nanostars"	514:524	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	3	4	theme	nanostars	515:523	arg1	particles					503:511	small DNA particles	493:511	small DNA particles ("nanostars") whose phase separation is driven by base pairing	493:574	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	4	5	theme	characteristic	709:722	arg1	size					729:732	the liquid's characteristic mesh size	696:732	the liquid's characteristic mesh size	696:732	We find that linker partitioning is length-dependent because of a confinement penalty of inserting long strands within the liquid's characteristic mesh size.					
31638820	6	6	theme	long	978:981	arg1	linkers					983:989	long linkers	978:989	long linkers excluded from the liquid interior	978:1023	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	6	7	theme	high	1148:1151	arg1	concentrations					1153:1166	high concentrations	1148:1166	high concentrations	1148:1166	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	4	8	theme	inserting	666:674	arg1	strands					681:687	inserting long strands	666:687	inserting long strands within the liquid's characteristic mesh size	666:732	We find that linker partitioning is length-dependent because of a confinement penalty of inserting long strands within the liquid's characteristic mesh size.					
31638820	5	9	theme	confined	888:895	arg1	chains					907:912	confined worm-like chains	888:912	confined worm-like chains	888:912	We quantify this entropic-confinement effect using a simple partitioning theory and show that its magnitude is consistent with classic Odijk pictures of confined worm-like chains.					
31638820	6	10	theme	droplet-in-droplet	1241:1258	arg1	structures					1260:1269	metastable droplet-in-droplet structures	1230:1269	metastable droplet-in-droplet structures	1230:1269	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	3	11	theme	linkers	459:465	arg1	partitioning					418:429	the partitioning	414:429	the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing	414:574	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	6	12	dep	accumulate	1048:1057	arg1	i.e.					1094:1097	i.e.	1094:1097	i.e.	1094:1097	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	3	13	from	goals	383:387	arg1	mind					392:395	mind	392:395	mind	392:395	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	6	14	theme	metastable	1230:1239	arg1	structures					1260:1269	metastable droplet-in-droplet structures	1230:1269	metastable droplet-in-droplet structures	1230:1269	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	2	15	theme	process	283:289	arg1	mechanisms					264:273	the mechanisms	260:273	the mechanisms of this process	260:289	Synthetic biomolecular liquids offer a means to study the mechanisms of this process, as well as offering a route to the creation of functional biomimetic materials.					
31638820	6	16	theme	liquid	1077:1082	arg1	droplets					1084:1091	liquid droplets	1077:1091	liquid droplets	1077:1091	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	1	17	theme	membrane-less	180:192	arg1	organelles					194:203	membrane-less organelles	180:203	membrane-less organelles	180:203	Cells can spatially and temporally control biochemistry using liquid-liquid phase separation to form membrane-less organelles.					
31638820	3	18	theme	phase	533:537	arg1	separation					539:548	separation	539:548	separation	539:548	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	5	19	theme	simple	788:793	arg1	theory					808:813	a simple partitioning theory	786:813	a simple partitioning theory	786:813	We quantify this entropic-confinement effect using a simple partitioning theory and show that its magnitude is consistent with classic Odijk pictures of confined worm-like chains.					
31638820	5	20	theme	Odijk	870:874	arg1	pictures					876:883	classic Odijk pictures	862:883	classic Odijk pictures of confined worm-like chains	862:912	We quantify this entropic-confinement effect using a simple partitioning theory and show that its magnitude is consistent with classic Odijk pictures of confined worm-like chains.					
31638820	5	21	theme	partitioning	795:806	arg1	theory					808:813	a simple partitioning theory	786:813	a simple partitioning theory	786:813	We quantify this entropic-confinement effect using a simple partitioning theory and show that its magnitude is consistent with classic Odijk pictures of confined worm-like chains.					
31638820	6	22	theme	inhomogeneous	952:964	arg1	structures					966:975	inhomogeneous structures	952:975	inhomogeneous structures	952:975	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	4	23	theme	mesh	724:727	arg1	size					729:732	the liquid's characteristic mesh size	696:732	the liquid's characteristic mesh size	696:732	We find that linker partitioning is length-dependent because of a confinement penalty of inserting long strands within the liquid's characteristic mesh size.					
31638820	3	24	theme	DNA	455:457	arg1	linkers					459:465	long double-stranded DNA linkers	434:465	long double-stranded DNA linkers	434:465	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	0	25	theme	Spatial	22:28	arg1	Structure					30:38	Spatial Structure	22:38	Spatial Structure	22:38	Length-Dependence and Spatial Structure of DNA Partitioning into a DNA Liquid.					
31638820	2	26	theme	functional	339:348	arg1	materials					361:369	functional biomimetic materials	339:369	functional biomimetic materials	339:369	Synthetic biomolecular liquids offer a means to study the mechanisms of this process, as well as offering a route to the creation of functional biomimetic materials.					
31638820	1	27	theme	liquid-liquid	141:153	arg1	separation					161:170	liquid-liquid phase separation	141:170	liquid-liquid phase separation	141:170	Cells can spatially and temporally control biochemistry using liquid-liquid phase separation to form membrane-less organelles.					
31638820	0	28	theme	DNA	43:45	arg1	Partitioning					47:58	DNA Partitioning	43:58	DNA Partitioning	43:58	Length-Dependence and Spatial Structure of DNA Partitioning into a DNA Liquid.					
31638820	5	29	theme	worm-like	897:905	arg1	chains					907:912	confined worm-like chains	888:912	confined worm-like chains	888:912	We quantify this entropic-confinement effect using a simple partitioning theory and show that its magnitude is consistent with classic Odijk pictures of confined worm-like chains.					
31638820	7	30	theme	model	1376:1380	arg1	structure					1361:1369	structure	1361:1369	structure	1361:1369	Altogether, our work demonstrates the ability to rationally engineer the composition and structure of a model biomolecular liquid.					
31638820	7	30	theme	model	1376:1380	arg1	composition					1345:1355	composition	1345:1355	composition	1345:1355	Altogether, our work demonstrates the ability to rationally engineer the composition and structure of a model biomolecular liquid.					
31638820	3	31	theme	long	434:437	arg1	linkers					459:465	long double-stranded DNA linkers	434:465	long double-stranded DNA linkers	434:465	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	5	32	theme	entropic-confinement	752:771	arg1	effect					773:778	this entropic-confinement effect	747:778	this entropic-confinement effect using a simple partitioning theory	747:813	We quantify this entropic-confinement effect using a simple partitioning theory and show that its magnitude is consistent with classic Odijk pictures of confined worm-like chains.					
31638820	3	33	theme	base	563:566	arg1	pairing					568:574	base pairing	563:574	base pairing	563:574	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	3	34	theme	double-stranded	439:453	arg1	linkers					459:465	long double-stranded DNA linkers	434:465	long double-stranded DNA linkers	434:465	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	5	35	theme	classic	862:868	arg1	pictures					876:883	classic Odijk pictures	862:883	classic Odijk pictures of confined worm-like chains	862:912	We quantify this entropic-confinement effect using a simple partitioning theory and show that its magnitude is consistent with classic Odijk pictures of confined worm-like chains.					
31638820	5	36	with	consistent	846:855	arg1	pictures					876:883	classic Odijk pictures	862:883	classic Odijk pictures of confined worm-like chains	862:912	We quantify this entropic-confinement effect using a simple partitioning theory and show that its magnitude is consistent with classic Odijk pictures of confined worm-like chains.					
31638820	6	37	theme	secondary	1194:1202	arg1	separation					1210:1219	a secondary phase separation	1192:1219	a secondary phase separation	1192:1219	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	4	38	theme	strands	681:687	arg1	penalty					655:661	a confinement penalty	641:661	a confinement penalty of inserting long strands within the liquid's characteristic mesh size	641:732	We find that linker partitioning is length-dependent because of a confinement penalty of inserting long strands within the liquid's characteristic mesh size.					
31638820	0	39	theme	Partitioning	47:58	arg1	Length-Dependence					0:16	Length-Dependence	0:16	Length-Dependence	0:16	Length-Dependence and Spatial Structure of DNA Partitioning into a DNA Liquid.					
31638820	0	39	theme	Partitioning	47:58	arg1	Structure					30:38	Spatial Structure	22:38	Spatial Structure	22:38	Length-Dependence and Spatial Structure of DNA Partitioning into a DNA Liquid.					
31638820	6	40	theme	phase	1204:1208	arg1	separation					1210:1219	a secondary phase separation	1192:1219	a secondary phase separation	1192:1219	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	4	41	theme	long	676:679	arg1	strands					681:687	inserting long strands	666:687	inserting long strands within the liquid's characteristic mesh size	666:732	We find that linker partitioning is length-dependent because of a confinement penalty of inserting long strands within the liquid's characteristic mesh size.					
31638820	6	42	theme	Linker	915:920	arg1	partitioning					922:933	Linker partitioning	915:933	Linker partitioning	915:933	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	4	43	theme	confinement	643:653	arg1	penalty					655:661	a confinement penalty	641:661	a confinement penalty of inserting long strands within the liquid's characteristic mesh size	641:732	We find that linker partitioning is length-dependent because of a confinement penalty of inserting long strands within the liquid's characteristic mesh size.					
31638820	5	44	theme	chains	907:912	arg1	pictures					876:883	classic Odijk pictures	862:883	classic Odijk pictures of confined worm-like chains	862:912	We quantify this entropic-confinement effect using a simple partitioning theory and show that its magnitude is consistent with classic Odijk pictures of confined worm-like chains.					
31638820	7	45	theme	liquid	1395:1400	arg1	model					1376:1380	a model	1374:1380	a model biomolecular liquid	1374:1400	Altogether, our work demonstrates the ability to rationally engineer the composition and structure of a model biomolecular liquid.					
31638820	2	46	theme	biomolecular	216:227	arg1	liquids					229:235	Synthetic biomolecular liquids	206:235	Synthetic biomolecular liquids	206:235	Synthetic biomolecular liquids offer a means to study the mechanisms of this process, as well as offering a route to the creation of functional biomimetic materials.					
31638820	7	47	dep	composition	1345:1355	arg1	the					1341:1343	the	1341:1343	the	1341:1343	Altogether, our work demonstrates the ability to rationally engineer the composition and structure of a model biomolecular liquid.					
31638820	1	48	theme	phase	155:159	arg1	separation					161:170	liquid-liquid phase separation	141:170	liquid-liquid phase separation	141:170	Cells can spatially and temporally control biochemistry using liquid-liquid phase separation to form membrane-less organelles.					
31638820	6	49	theme	liquid	1009:1014	arg1	interior					1016:1023	the liquid interior	1005:1023	the liquid interior	1005:1023	Linker partitioning can also lead to inhomogeneous structures: long linkers excluded from the liquid interior tend to preferentially accumulate on the surface of liquid droplets (i.e., acting as surfactants), while linkers forced at high concentrations into the liquid undergo a secondary phase separation, forming metastable droplet-in-droplet structures.					
31638820	2	50	theme	Synthetic	206:214	arg1	liquids					229:235	Synthetic biomolecular liquids	206:235	Synthetic biomolecular liquids	206:235	Synthetic biomolecular liquids offer a means to study the mechanisms of this process, as well as offering a route to the creation of functional biomimetic materials.					
31638820	4	51	theme	linker	590:595	arg1	partitioning					597:608	linker partitioning	590:608	linker partitioning	590:608	We find that linker partitioning is length-dependent because of a confinement penalty of inserting long strands within the liquid's characteristic mesh size.					
31638820	3	52	theme	DNA	499:501	arg1	"					524:524	"nanostars"	514:524	"nanostars"	514:524	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	3	52	theme	DNA	499:501	arg1	particles					503:511	small DNA particles	493:511	small DNA particles ("nanostars") whose phase separation is driven by base pairing	493:574	With these goals in mind, we here examine the partitioning of long double-stranded DNA linkers into a liquid composed of small DNA particles ("nanostars") whose phase separation is driven by base pairing.					
31638820	2	53	theme	materials	361:369	arg1	creation					327:334	the creation	323:334	the creation of functional biomimetic materials	323:369	Synthetic biomolecular liquids offer a means to study the mechanisms of this process, as well as offering a route to the creation of functional biomimetic materials.					
29412914	1	0	theme	resulting	341:349	arg1	wines					351:355	the resulting wines	337:355	the resulting wines	337:355	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	0	1	theme	immobilized	58:68	arg1	forms					84:88	free, bioimmobilized or immobilized with alginate forms	34:88	free, bioimmobilized or immobilized with alginate forms	34:88	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	4	2	theme	overall	843:849	arg1	value					867:871	the overall aromatic series value	839:871	the overall aromatic series value	839:871	Taken together these contribute more than 70% of the overall aromatic series value.					
29412914	2	3	dep	cells	425:429	arg1	bioimmobilized					464:477	bioimmobilized	464:477	bioimmobilized as biocapsules	464:492	Sparkling wines obtained with two yeast strains inoculated as free cells, immobilized in alginate bed and bioimmobilized as biocapsules, were aged during 32 months.					
29412914	2	3	dep	cells	425:429	arg1	immobilized					432:442	immobilized	432:442	immobilized in alginate bed	432:458	Sparkling wines obtained with two yeast strains inoculated as free cells, immobilized in alginate bed and bioimmobilized as biocapsules, were aged during 32 months.					
29412914	5	4	theme	sparkling	1059:1067	arg1	wines					1069:1073	sparkling wines	1059:1073	sparkling wines	1059:1073	Although some results rely more on the yeast strain than the inoculation format, specific aroma compounds were associated with the immobilization format, allowing the classification of sparkling wines by PCA.					
29412914	5	5	theme	immobilization	1005:1018	arg1	format					1020:1025	the immobilization format	1001:1025	the immobilization format	1001:1025	Although some results rely more on the yeast strain than the inoculation format, specific aroma compounds were associated with the immobilization format, allowing the classification of sparkling wines by PCA.					
29412914	4	6	theme	series	860:865	arg1	value					867:871	the overall aromatic series value	839:871	the overall aromatic series value	839:871	Taken together these contribute more than 70% of the overall aromatic series value.					
29412914	1	7	theme	sensory	318:324	arg1	quality					326:332	sensory quality	318:332	sensory quality	318:332	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	6	8	theme	immobilized	1156:1166	arg1	yeasts					1168:1173	immobilized yeasts	1156:1173	immobilized yeasts	1156:1173	As a result the aroma quality of sparkling wines could be improved using immobilized yeasts.					
29412914	6	9	theme	sparkling	1116:1124	arg1	wines					1126:1130	sparkling wines	1116:1130	sparkling wines	1116:1130	As a result the aroma quality of sparkling wines could be improved using immobilized yeasts.					
29412914	3	10	theme	volatile	533:540	arg1	compounds					542:550	the volatile compounds	529:550	the volatile compounds	529:550	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	3	11	theme	ethyl	692:696	arg1	octanoate					698:706	ethyl octanoate	692:706	ethyl octanoate	692:706	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	5	12	theme	yeast	913:917	arg1	strain					919:924	the yeast strain	909:924	the yeast strain	909:924	Although some results rely more on the yeast strain than the inoculation format, specific aroma compounds were associated with the immobilization format, allowing the classification of sparkling wines by PCA.					
29412914	0	13	theme	free	34:37	arg1	forms					84:88	free, bioimmobilized or immobilized with alginate forms	34:88	free, bioimmobilized or immobilized with alginate forms	34:88	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	2	14	theme	alginate	447:454	arg1	bed					456:458	alginate bed	447:458	alginate bed	447:458	Sparkling wines obtained with two yeast strains inoculated as free cells, immobilized in alginate bed and bioimmobilized as biocapsules, were aged during 32 months.					
29412914	5	15	theme	wines	1069:1073	arg1	classification					1041:1054	the classification	1037:1054	the classification of sparkling wines	1037:1073	Although some results rely more on the yeast strain than the inoculation format, specific aroma compounds were associated with the immobilization format, allowing the classification of sparkling wines by PCA.					
29412914	6	16	theme	aroma	1099:1103	arg1	result					1088:1093	a result	1086:1093	a result	1086:1093	As a result the aroma quality of sparkling wines could be improved using immobilized yeasts.					
29412914	6	16	theme	aroma	1099:1103	arg1	quality					1105:1111	the aroma quality	1095:1111	the aroma quality of sparkling wines	1095:1130	As a result the aroma quality of sparkling wines could be improved using immobilized yeasts.					
29412914	2	17	theme	Sparkling	358:366	arg1	wines					368:372	Sparkling wines	358:372	Sparkling wines obtained with two yeast strains inoculated as free cells, immobilized in alginate bed and bioimmobilized as biocapsules,	358:493	Sparkling wines obtained with two yeast strains inoculated as free cells, immobilized in alginate bed and bioimmobilized as biocapsules, were aged during 32 months.					
29412914	2	18	theme	free	420:423	arg1	cells					425:429	free cells	420:429	free cells	420:429	Sparkling wines obtained with two yeast strains inoculated as free cells, immobilized in alginate bed and bioimmobilized as biocapsules, were aged during 32 months.					
29412914	0	19	from	strains	23:29	arg1	forms					84:88	free, bioimmobilized or immobilized with alginate forms	34:88	free, bioimmobilized or immobilized with alginate forms	34:88	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	5	20	theme	inoculation	935:945	arg1	format					947:952	the inoculation format	931:952	the inoculation format	931:952	Although some results rely more on the yeast strain than the inoculation format, specific aroma compounds were associated with the immobilization format, allowing the classification of sparkling wines by PCA.					
29412914	1	21	dep	composition	302:312	arg1	the					292:294	the	292:294	the	292:294	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	1	22	theme	sparkling	158:166	arg1	wines					168:172	sparkling wines	158:172	sparkling wines	158:172	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	1	23	theme	extended	250:257	arg1	period					259:264	an extended period	247:264	an extended period of time	247:272	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	1	24	theme	wines	168:172	arg1	Production					144:153	Production	144:153	Production of sparkling wines	144:172	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	0	25	theme	yeast	17:21	arg1	strains					23:29	two yeast strains	13:29	two yeast strains in free, bioimmobilized or immobilized with alginate forms	13:88	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	3	26	theme	ethyl	650:654	arg1	3-methylbutanoate					656:672	ethyl 3-methylbutanoate	650:672	ethyl 3-methylbutanoate	650:672	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	4	27	theme	value	867:871	arg1	value					867:871	the overall aromatic series value	839:871	the overall aromatic series value	839:871	Taken together these contribute more than 70% of the overall aromatic series value.					
29412914	4	27	theme	value	867:871	arg1	%					834:834	more than 70%	822:834	more than 70% of the overall aromatic series value	822:871	Taken together these contribute more than 70% of the overall aromatic series value.					
29412914	2	28	theme	yeast	392:396	arg1	strains					398:404	two yeast strains	388:404	two yeast strains inoculated as free cells, immobilized in alginate bed and bioimmobilized as biocapsules,	388:493	Sparkling wines obtained with two yeast strains inoculated as free cells, immobilized in alginate bed and bioimmobilized as biocapsules, were aged during 32 months.					
29412914	0	29	theme	aromatic	97:104	arg1	profile					106:112	the aromatic profile	93:112	the aromatic profile of long aged sparkling wines	93:141	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	1	30	theme	wines	351:355	arg1	composition					302:312	aroma composition	296:312	aroma composition	296:312	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	1	30	theme	wines	351:355	arg1	quality					326:332	sensory quality	318:332	sensory quality	318:332	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	1	31	theme	time	269:272	arg1	period					259:264	an extended period	247:264	an extended period of time	247:272	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	0	32	theme	long	117:120	arg1	wines					137:141	long aged sparkling wines	117:141	long aged sparkling wines	117:141	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	3	33	theme	ethyl	615:619	arg1	propanoate					621:630	ethyl propanoate	615:630	ethyl propanoate	615:630	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	3	34	theme	Odor	558:561	arg1	Values					572:577	high Odor Activity Values	553:577	high Odor Activity Values	553:577	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	1	35	theme	second	184:189	arg1	fermentation					201:212	a second alcoholic fermentation	182:212	a second alcoholic fermentation	182:212	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	0	36	theme	strains	23:29	arg1	Influence					0:8	Influence	0:8	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.	0:142	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	3	37	theme	isoamyl	598:604	arg1	acetate					606:612	isoamyl acetate	598:612	isoamyl acetate	598:612	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	3	38	theme	Activity	563:570	arg1	Values					572:577	high Odor Activity Values	553:577	high Odor Activity Values	553:577	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	1	39	theme	alcoholic	191:199	arg1	fermentation					201:212	a second alcoholic fermentation	182:212	a second alcoholic fermentation	182:212	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	0	40	theme	sparkling	127:135	arg1	wines					137:141	long aged sparkling wines	117:141	long aged sparkling wines	117:141	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	5	41	theme	aroma	964:968	arg1	compounds					970:978	specific aroma compounds	955:978	specific aroma compounds	955:978	Although some results rely more on the yeast strain than the inoculation format, specific aroma compounds were associated with the immobilization format, allowing the classification of sparkling wines by PCA.					
29412914	3	42	theme	ethyl	675:679	arg1	hexanoate					681:689	ethyl hexanoate	675:689	ethyl hexanoate	675:689	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	0	43	from	Influence	0:8	arg1	forms					84:88	free, bioimmobilized or immobilized with alginate forms	34:88	free, bioimmobilized or immobilized with alginate forms	34:88	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	0	43	from	Influence	0:8	arg1	profile					106:112	the aromatic profile	93:112	the aromatic profile of long aged sparkling wines	93:141	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	0	44	theme	aged	122:125	arg1	wines					137:141	long aged sparkling wines	117:141	long aged sparkling wines	117:141	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	3	45	theme	ethyl	633:637	arg1	butanoate					639:647	ethyl butanoate	633:647	ethyl butanoate	633:647	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	3	46	theme	high	553:556	arg1	Values					572:577	high Odor Activity Values	553:577	high Odor Activity Values	553:577	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	4	47	theme	aromatic	851:858	arg1	value					867:871	the overall aromatic series value	839:871	the overall aromatic series value	839:871	Taken together these contribute more than 70% of the overall aromatic series value.					
29412914	5	48	theme	specific	955:962	arg1	compounds					970:978	specific aroma compounds	955:978	specific aroma compounds	955:978	Although some results rely more on the yeast strain than the inoculation format, specific aroma compounds were associated with the immobilization format, allowing the classification of sparkling wines by PCA.					
29412914	0	49	theme	wines	137:141	arg1	profile					106:112	the aromatic profile	93:112	the aromatic profile of long aged sparkling wines	93:141	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	0	50	from	forms	84:88	arg1	Influence					0:8	Influence	0:8	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.	0:142	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	1	51	with	contact	218:224	arg1	yeast					231:235	yeast	231:235	yeast	231:235	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	3	52	theme	decanoic	767:774	arg1	acid					776:779	decanoic acid	767:779	decanoic acid	767:779	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	1	53	dep	involve	174:180	arg1	influences					281:290	influences	281:290	influences the aroma composition and sensory quality of the resulting wines	281:355	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	3	54	theme	octanoic	752:759	arg1	acid					761:764	octanoic acid	752:764	octanoic acid	752:764	Among the volatile compounds, high Odor Activity Values were obtained with isoamyl acetate, ethyl propanoate, ethyl butanoate, ethyl 3-methylbutanoate, ethyl hexanoate, ethyl octanoate, hexanol, 2-methoxy-4-vinylphenol, decanal, octanoic acid, decanoic acid and TDN.					
29412914	0	55	theme	bioimmobilized	40:53	arg1	forms					84:88	free, bioimmobilized or immobilized with alginate forms	34:88	free, bioimmobilized or immobilized with alginate forms	34:88	Influence of two yeast strains in free, bioimmobilized or immobilized with alginate forms on the aromatic profile of long aged sparkling wines.					
29412914	1	56	theme	aroma	296:300	arg1	composition					302:312	aroma composition	296:312	aroma composition	296:312	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	6	57	theme	wines	1126:1130	arg1	result					1088:1093	a result	1086:1093	a result	1086:1093	As a result the aroma quality of sparkling wines could be improved using immobilized yeasts.					
29412914	6	57	theme	wines	1126:1130	arg1	quality					1105:1111	the aroma quality	1095:1111	the aroma quality of sparkling wines	1095:1130	As a result the aroma quality of sparkling wines could be improved using immobilized yeasts.					
29412914	1	58	with	fermentation	201:212	arg1	yeast					231:235	yeast	231:235	yeast	231:235	Production of sparkling wines involve a second alcoholic fermentation and contact with yeast less over an extended period of time, which influences the aroma composition and sensory quality of the resulting wines.					
29412914	4	59	dep	contribute	811:820	arg1	Taken					790:794	Taken	790:794	Taken together	790:803	Taken together these contribute more than 70% of the overall aromatic series value.					
29392453	0	0	theme	pretreated	92:101	arg1	straw					109:113	pretreated wheat straw	92:113	pretreated wheat straw	92:113	Properties important for solid-liquid separations change during the enzymatic hydrolysis of pretreated wheat straw.					
29392453	5	1	dep	RESULTS	825:831	arg1	measurements					855:866	Water retention value measurements	833:866	RESULTS Water retention value measurements	825:866	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	7	2	from	fraction	1436:1443	arg1	separate					1391:1398	separate	1391:1398	separate	1391:1398	CONCLUSIONS With increased water retained by the insoluble fraction as cellulose hydrolysis proceeds, it may prove more difficult to efficiently separate hydrolysis residues from the liquid fraction with improved hydrolysis.					
29392453	4	3	theme	hydrolysis	699:708	arg1	effect					679:684	the effect	675:684	the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass	675:779	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	2	4	theme	product	368:374	arg1	recovery					376:383	efficient product recovery	358:383	efficient product recovery	358:383	One such process, which has received little attention, but is of great importance for efficient product recovery, is solid-liquid separations, which may occur both after pretreatment and after the enzymatic hydrolysis steps.					
29392453	7	5	with	fraction	1436:1443	arg1	hydrolysis					1459:1468	improved hydrolysis	1450:1468	improved hydrolysis	1450:1468	CONCLUSIONS With increased water retained by the insoluble fraction as cellulose hydrolysis proceeds, it may prove more difficult to efficiently separate hydrolysis residues from the liquid fraction with improved hydrolysis.					
29392453	0	6	theme	straw	109:113	arg1	hydrolysis					78:87	the enzymatic hydrolysis	64:87	the enzymatic hydrolysis of pretreated wheat straw	64:113	Properties important for solid-liquid separations change during the enzymatic hydrolysis of pretreated wheat straw.					
29392453	6	7	theme	dominant	1221:1228	arg1	changes					1176:1182	changes	1176:1182	structural decomposition rather than changes in chemical composition	1139:1206	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	6	7	theme	dominant	1221:1228	arg1	decomposition					1150:1162	structural decomposition	1139:1162	structural decomposition rather than changes in chemical composition	1139:1206	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	6	7	theme	dominant	1221:1228	arg1	characteristic					1230:1243	the most dominant characteristic	1212:1243	the most dominant characteristic	1212:1243	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	4	8	theme	biomass	773:779	arg1	properties					748:757	the water retention properties	728:757	the water retention properties of pretreated biomass	728:779	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	3	9	theme	solid-liquid	568:579	arg1	properties					592:601	the solid-liquid separation properties	564:601	the solid-liquid separation properties of the biomass	564:616	Due to the changing nature of the solid biomass during processing, the solid-liquid separation properties of the biomass can also change.					
29392453	0	10	theme	wheat	103:107	arg1	straw					109:113	pretreated wheat straw	92:113	pretreated wheat straw	92:113	Properties important for solid-liquid separations change during the enzymatic hydrolysis of pretreated wheat straw.					
29392453	6	11	theme	structural	1139:1148	arg1	changes					1176:1182	changes	1176:1182	structural decomposition rather than changes in chemical composition	1139:1206	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	6	11	theme	structural	1139:1148	arg1	decomposition					1150:1162	structural decomposition	1139:1162	structural decomposition rather than changes in chemical composition	1139:1206	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	6	11	theme	structural	1139:1148	arg1	characteristic					1230:1243	the most dominant characteristic	1212:1243	the most dominant characteristic	1212:1243	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	4	12	theme	pretreated	762:771	arg1	biomass					773:779	pretreated biomass	762:779	pretreated biomass	762:779	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	2	13	theme	little	309:314	arg1	attention					316:324	little attention	309:324	little attention	309:324	One such process, which has received little attention, but is of great importance for efficient product recovery, is solid-liquid separations, which may occur both after pretreatment and after the enzymatic hydrolysis steps.					
29392453	7	14	theme	liquid	1429:1434	arg1	fraction					1436:1443	the liquid fraction	1425:1443	the liquid fraction with improved hydrolysis	1425:1468	CONCLUSIONS With increased water retained by the insoluble fraction as cellulose hydrolysis proceeds, it may prove more difficult to efficiently separate hydrolysis residues from the liquid fraction with improved hydrolysis.					
29392453	4	15	theme	enzymatic	689:697	arg1	hydrolysis					699:708	enzymatic hydrolysis	689:708	enzymatic hydrolysis of cellulose	689:721	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	4	16	theme	cellulose	713:721	arg1	hydrolysis					699:708	enzymatic hydrolysis	689:708	enzymatic hydrolysis of cellulose	689:721	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	5	17	theme	value	849:853	arg1	measurements					855:866	Water retention value measurements	833:866	RESULTS Water retention value measurements	825:866	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	4	18	theme	retention	738:746	arg1	properties					748:757	the water retention properties	728:757	the water retention properties of pretreated biomass	728:779	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	3	19	theme	biomass	537:543	arg1	nature					517:522	the changing nature	504:522	the changing nature of the solid biomass during processing	504:561	Due to the changing nature of the solid biomass during processing, the solid-liquid separation properties of the biomass can also change.					
29392453	6	20	theme	fines	1075:1079	arg1	fraction					1081:1088	the fines fraction	1071:1088	the fines fraction	1071:1088	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	5	21	from	increase	920:927	arg1	retention					938:946	water retention	932:946	water retention	932:946	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	5	22	theme	water	932:936	arg1	retention					938:946	water retention	932:946	water retention	932:946	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	0	23	theme	important	11:19	arg1	Properties					0:9	Properties	0:9	Properties important for solid-liquid separations	0:48	Properties important for solid-liquid separations change during the enzymatic hydrolysis of pretreated wheat straw.					
29392453	3	24	theme	solid	531:535	arg1	biomass					537:543	the solid biomass	527:543	the solid biomass	527:543	Due to the changing nature of the solid biomass during processing, the solid-liquid separation properties of the biomass can also change.					
29392453	6	25	from	decrease	1096:1103	arg1	fraction					1081:1088	the fines fraction	1071:1088	the fines fraction	1071:1088	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	6	25	from	decrease	1096:1103	arg1	size					1117:1120	particle size	1108:1120	particle size	1108:1120	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	3	26	theme	separation	581:590	arg1	properties					592:601	the solid-liquid separation properties	564:601	the solid-liquid separation properties of the biomass	564:616	Due to the changing nature of the solid biomass during processing, the solid-liquid separation properties of the biomass can also change.					
29392453	6	27	from	changes	1176:1182	arg1	composition					1196:1206	chemical composition	1187:1206	chemical composition	1187:1206	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	5	28	theme	1H	882:883	arg1	NMR					885:887	1H NMR	882:887	1H NMR T2 relaxometry data	882:907	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	4	29	theme	water	732:736	arg1	properties					748:757	the water retention properties	728:757	the water retention properties of pretreated biomass	728:779	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	2	30	theme	solid-liquid	389:400	arg1	separations					402:412	solid-liquid separations	389:412	solid-liquid separations	389:412	One such process, which has received little attention, but is of great importance for efficient product recovery, is solid-liquid separations, which may occur both after pretreatment and after the enzymatic hydrolysis steps.					
29392453	0	31	theme	solid-liquid	25:36	arg1	separations					38:48	solid-liquid separations	25:48	solid-liquid separations	25:48	Properties important for solid-liquid separations change during the enzymatic hydrolysis of pretreated wheat straw.					
29392453	7	32	with	CONCLUSIONS	1246:1256	arg1	water					1273:1277	increased water	1263:1277	increased water	1263:1277	CONCLUSIONS With increased water retained by the insoluble fraction as cellulose hydrolysis proceeds, it may prove more difficult to efficiently separate hydrolysis residues from the liquid fraction with improved hydrolysis.					
29392453	3	33	theme	biomass	610:616	arg1	properties					592:601	the solid-liquid separation properties	564:601	the solid-liquid separation properties of the biomass	564:616	Due to the changing nature of the solid biomass during processing, the solid-liquid separation properties of the biomass can also change.					
29392453	1	34	theme	new	225:227	arg1	challenges					229:238	new challenges	225:238	new challenges for industrial scale processes	225:269	OBJECTIVES The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes.					
29392453	5	35	theme	Water	833:837	arg1	measurements					855:866	Water retention value measurements	833:866	RESULTS Water retention value measurements	825:866	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	1	36	theme	renewable	186:194	arg1	fuels					196:200	renewable fuels	186:200	renewable fuels	186:200	OBJECTIVES The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes.					
29392453	5	37	theme	T2	889:890	arg1	data					904:907	1H NMR T2 relaxometry data	882:907	1H NMR T2 relaxometry data	882:907	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	3	38	theme	changing	508:515	arg1	nature					517:522	the changing nature	504:522	the changing nature of the solid biomass during processing	504:561	Due to the changing nature of the solid biomass during processing, the solid-liquid separation properties of the biomass can also change.					
29392453	7	39	theme	increased	1263:1271	arg1	water					1273:1277	increased water	1263:1277	increased water	1263:1277	CONCLUSIONS With increased water retained by the insoluble fraction as cellulose hydrolysis proceeds, it may prove more difficult to efficiently separate hydrolysis residues from the liquid fraction with improved hydrolysis.					
29392453	1	40	dep	chemicals	206:214	arg1	provides					216:223	provides	216:223	provides new challenges for industrial scale processes	216:269	OBJECTIVES The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes.					
29392453	5	41	theme	NMR	885:887	arg1	data					904:907	1H NMR T2 relaxometry data	882:907	1H NMR T2 relaxometry data	882:907	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	4	42	theme	study	657:661	arg1	objective					639:647	The objective	635:647	The objective of this study	635:661	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	4	43	theme	reaction	815:822	arg1	course					790:795	the course	786:795	the course of the hydrolysis reaction	786:822	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	1	44	theme	industrial	244:253	arg1	processes					261:269	industrial scale processes	244:269	industrial scale processes	244:269	OBJECTIVES The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes.					
29392453	2	45	theme	hydrolysis	479:488	arg1	steps					490:494	the enzymatic hydrolysis steps	465:494	the enzymatic hydrolysis steps	465:494	One such process, which has received little attention, but is of great importance for efficient product recovery, is solid-liquid separations, which may occur both after pretreatment and after the enzymatic hydrolysis steps.					
29392453	5	46	theme	hydrolysis	1023:1032	arg1	levels					1003:1008	levels	1003:1008	levels of cellulose hydrolysis	1003:1032	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	2	47	theme	efficient	358:366	arg1	recovery					376:383	efficient product recovery	358:383	efficient product recovery	358:383	One such process, which has received little attention, but is of great importance for efficient product recovery, is solid-liquid separations, which may occur both after pretreatment and after the enzymatic hydrolysis steps.					
29392453	2	48	theme	enzymatic	469:477	arg1	steps					490:494	the enzymatic hydrolysis steps	465:494	the enzymatic hydrolysis steps	465:494	One such process, which has received little attention, but is of great importance for efficient product recovery, is solid-liquid separations, which may occur both after pretreatment and after the enzymatic hydrolysis steps.					
29392453	7	49	theme	hydrolysis	1400:1409	arg1	residues					1411:1418	hydrolysis residues	1400:1418	hydrolysis residues	1400:1418	CONCLUSIONS With increased water retained by the insoluble fraction as cellulose hydrolysis proceeds, it may prove more difficult to efficiently separate hydrolysis residues from the liquid fraction with improved hydrolysis.					
29392453	6	50	theme	most	1216:1219	arg1	changes					1176:1182	changes	1176:1182	structural decomposition rather than changes in chemical composition	1139:1206	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	6	50	theme	most	1216:1219	arg1	decomposition					1150:1162	structural decomposition	1139:1162	structural decomposition rather than changes in chemical composition	1139:1206	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	6	50	theme	most	1216:1219	arg1	characteristic					1230:1243	the most dominant characteristic	1212:1243	the most dominant characteristic	1212:1243	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	1	51	theme	biochemical	131:141	arg1	conversion					143:152	The biochemical conversion	127:152	The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes	127:269	OBJECTIVES The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes.					
29392453	5	52	theme	cellulose	1013:1021	arg1	hydrolysis					1023:1032	cellulose hydrolysis	1013:1032	cellulose hydrolysis	1013:1032	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	6	53	theme	particle	1108:1115	arg1	size					1117:1120	particle size	1108:1120	particle size	1108:1120	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	5	54	from	constraint	952:961	arg1	retention					938:946	water retention	932:946	water retention	932:946	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	1	55	theme	scale	255:259	arg1	processes					261:269	industrial scale processes	244:269	industrial scale processes	244:269	OBJECTIVES The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes.					
29392453	7	56	theme	improved	1450:1457	arg1	hydrolysis					1459:1468	improved hydrolysis	1450:1468	improved hydrolysis	1450:1468	CONCLUSIONS With increased water retained by the insoluble fraction as cellulose hydrolysis proceeds, it may prove more difficult to efficiently separate hydrolysis residues from the liquid fraction with improved hydrolysis.					
29392453	7	57	theme	insoluble	1295:1303	arg1	fraction					1305:1312	the insoluble fraction	1291:1312	the insoluble fraction	1291:1312	CONCLUSIONS With increased water retained by the insoluble fraction as cellulose hydrolysis proceeds, it may prove more difficult to efficiently separate hydrolysis residues from the liquid fraction with improved hydrolysis.					
29392453	2	58	theme	great	337:341	arg1	importance					343:352	great importance	337:352	great importance	337:352	One such process, which has received little attention, but is of great importance for efficient product recovery, is solid-liquid separations, which may occur both after pretreatment and after the enzymatic hydrolysis steps.					
29392453	5	59	theme	relaxometry	892:902	arg1	data					904:907	1H NMR T2 relaxometry data	882:907	1H NMR T2 relaxometry data	882:907	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	4	60	theme	hydrolysis	804:813	arg1	reaction					815:822	the hydrolysis reaction	800:822	the hydrolysis reaction	800:822	The objective of this study was to show the effect of enzymatic hydrolysis of cellulose upon the water retention properties of pretreated biomass over the course of the hydrolysis reaction.					
29392453	5	61	theme	retention	839:847	arg1	measurements					855:866	Water retention value measurements	833:866	RESULTS Water retention value measurements	825:866	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	1	62	theme	lignocellulosic	157:171	arg1	biomass					173:179	lignocellulosic biomass	157:179	lignocellulosic biomass	157:179	OBJECTIVES The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes.					
29392453	7	63	theme	cellulose	1317:1325	arg1	hydrolysis					1327:1336	cellulose hydrolysis	1317:1336	cellulose hydrolysis	1317:1336	CONCLUSIONS With increased water retained by the insoluble fraction as cellulose hydrolysis proceeds, it may prove more difficult to efficiently separate hydrolysis residues from the liquid fraction with improved hydrolysis.					
29392453	0	64	theme	enzymatic	68:76	arg1	hydrolysis					78:87	the enzymatic hydrolysis	64:87	the enzymatic hydrolysis of pretreated wheat straw	64:113	Properties important for solid-liquid separations change during the enzymatic hydrolysis of pretreated wheat straw.					
29392453	5	65	theme	water	966:970	arg1	constraint					952:961	constraint	952:961	constraint of water	952:970	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	5	65	theme	water	966:970	arg1	increase					920:927	an increase	917:927	an increase in water retention	917:946	RESULTS Water retention value measurements, coupled with 1H NMR T2 relaxometry data, showed an increase in water retention and constraint of water by the biomass with increasing levels of cellulose hydrolysis.					
29392453	6	66	theme	chemical	1187:1194	arg1	composition					1196:1206	chemical composition	1187:1206	chemical composition	1187:1206	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	6	67	from	increase	1059:1066	arg1	fraction					1081:1088	the fines fraction	1071:1088	the fines fraction	1071:1088	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	6	67	from	increase	1059:1066	arg1	size					1117:1120	particle size	1108:1120	particle size	1108:1120	This correlated with an increase in the fines fraction and a decrease in particle size, suggesting that structural decomposition rather than changes in chemical composition was the most dominant characteristic.					
29392453	1	68	theme	biomass	173:179	arg1	conversion					143:152	The biochemical conversion	127:152	The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes	127:269	OBJECTIVES The biochemical conversion of lignocellulosic biomass into renewable fuels and chemicals provides new challenges for industrial scale processes.					
30593802	1	0	theme	fabricated	217:226	arg1	Co3O4					228:232	green fabricated Co3O4	211:232	green fabricated Co3O4	211:232	Bentonite/chitosan composite supported by green fabricated Co3O4 was successfully synthesized and its physicochemical properties were investigated utilizing several analytic techniques.					
30593802	3	1	dep	Co3O4	508:512	arg1	ions					552:555	ions	552:555	ions	552:555	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions were investigated.					
30593802	7	2	theme	adsorption	1020:1029	arg1	qmax					1041:1044	qmax	1041:1044	qmax	1041:1044	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	2	theme	adsorption	1020:1029	arg1	303 mg/g					1050:1057	303 mg/g	1050:1057	303 mg/g	1050:1057	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	2	theme	adsorption	1020:1029	arg1	capacity					1031:1038	The theoretical calculated maximum adsorption capacity	985:1038	The theoretical calculated maximum adsorption capacity (qmax)	985:1045	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	10	3	theme	promising	1546:1554	arg1	adsorbents					1556:1565	the promising adsorbents	1542:1565	the promising adsorbents	1542:1565	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	3	4	dep	Cr	545:546	arg1	VI					548:549	VI	548:549	VI	548:549	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions were investigated.					
30593802	7	5	theme	calculated	1001:1010	arg1	qmax					1041:1044	qmax	1041:1044	qmax	1041:1044	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	5	theme	calculated	1001:1010	arg1	303 mg/g					1050:1057	303 mg/g	1050:1057	303 mg/g	1050:1057	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	5	theme	calculated	1001:1010	arg1	capacity					1031:1038	The theoretical calculated maximum adsorption capacity	985:1038	The theoretical calculated maximum adsorption capacity (qmax)	985:1045	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	9	6	theme	oxidation	1294:1302	arg1	properties					1304:1313	high oxidation properties	1289:1313	high oxidation properties	1289:1313	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	8	7	theme	red	1239:1241	arg1	molecules					1243:1251	Congo red molecules	1233:1251	Congo red molecules	1233:1251	The composite is of high reusability and can be used effectively for six runs of decontamination of Congo red molecules and Cr(VI) ions.					
30593802	10	8	theme	@	1446:1446	arg1	composite					1459:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	0	9	theme	oxidation	110:118	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	8	10	used	used	1181:1184	arg2	composite					1137:1145	The composite	1133:1145	The composite	1133:1145	The composite is of high reusability and can be used effectively for six runs of decontamination of Congo red molecules and Cr(VI) ions.					
30593802	5	11	theme	excellent	771:779	arg1	fitting					781:787	excellent fitting	771:787	excellent fitting	771:787	Additionally, the systems are of chemisorption nature and showed excellent fitting with Pseudo-second order model.					
30593802	3	12	theme	Cr	545:546	arg1	properties					475:484	The adsorption properties	460:484	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions	460:555	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions were investigated.					
30593802	6	13	theme	models	865:870	arg1	parameters					838:847	The mathematical parameters	821:847	The mathematical parameters of the isotherm models	821:870	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	8	14	theme	Cr	1257:1258	arg1	ions					1264:1267	Cr(VI) ions	1257:1267	Cr(VI) ions	1257:1267	The composite is of high reusability and can be used effectively for six runs of decontamination of Congo red molecules and Cr(VI) ions.					
30593802	0	15	theme	red	137:139	arg1	dye					141:143	Congo red dye	131:143	Congo red dye	131:143	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	3	16	dep	dye	525:527	arg1	red					536:538	red	536:538	red	536:538	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions were investigated.					
30593802	6	17	theme	ions	934:937	arg1	uptake					893:898	a monolayer uptake	881:898	a monolayer uptake of Congo red molecules and Cr(VI) ions	881:937	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	4	18	theme	composite	628:636	arg1	surface					638:644	the composite surface	624:644	the composite surface after 480 min	624:658	The kinetic studies reflected the saturation of the composite surface after 480 min for both Congo red molecules and Cr(VI) ions.					
30593802	0	19	theme	@	69:69	arg1	Co					70:71	BE/CH@Co	64:71	BE/CH@Co	64:71	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	19	theme	@	69:69	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	19	theme	@	69:69	arg1	composite					53:61	bentonite/chitosan@cobalt oxide composite	21:61	bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	21:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	1	20	theme	analytic	334:341	arg1	techniques					343:352	several analytic techniques	326:352	several analytic techniques	326:352	Bentonite/chitosan composite supported by green fabricated Co3O4 was successfully synthesized and its physicochemical properties were investigated utilizing several analytic techniques.					
30593802	9	21	theme	photocatalytic	1338:1351	arg1	reduction					1353:1361	the photocatalytic reduction	1334:1361	the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water	1334:1411	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	9	22	theme	red	1372:1374	arg1	molecules					1376:1384	Congo red molecules	1366:1384	Congo red molecules	1366:1384	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	6	23	theme	Cr	927:928	arg1	ions					934:937	Cr(VI) ions	927:937	Cr(VI) ions	927:937	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	6	24	dep	Cr	927:928	arg1	VI					930:931	VI	930:931	VI	930:931	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	8	25	theme	decontamination	1214:1228	arg1	runs					1206:1209	six runs	1202:1209	six runs of decontamination of Congo red molecules and Cr(VI) ions	1202:1267	The composite is of high reusability and can be used effectively for six runs of decontamination of Congo red molecules and Cr(VI) ions.					
30593802	6	26	theme	molecules	913:921	arg1	uptake					893:898	a monolayer uptake	881:898	a monolayer uptake of Congo red molecules and Cr(VI) ions	881:937	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	0	27	theme	enhanced	77:84	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	2	28	theme	HRTEM	426:430	arg1	images					432:437	HRTEM images	426:437	HRTEM images	426:437	The formation of the composite was confirmed by the XRD patterns, SEM, HRTEM images, and FT-IR analysis.					
30593802	8	29	theme	molecules	1243:1251	arg1	decontamination					1214:1228	decontamination	1214:1228	decontamination of Congo red molecules and Cr(VI) ions	1214:1267	The composite is of high reusability and can be used effectively for six runs of decontamination of Congo red molecules and Cr(VI) ions.					
30593802	2	30	theme	composite	376:384	arg1	formation					359:367	The formation	355:367	The formation of the composite	355:384	The formation of the composite was confirmed by the XRD patterns, SEM, HRTEM images, and FT-IR analysis.					
30593802	0	31	theme	Green	0:4	arg1	fabrication					6:16	Green fabrication	0:16	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.	0:167	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	6	32	theme	Langmuir	969:976	arg1	model					978:982	the Langmuir model	965:982	the Langmuir model	965:982	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	4	33	theme	Cr	693:694	arg1	ions					700:703	Cr(VI) ions	693:703	Cr(VI) ions	693:703	The kinetic studies reflected the saturation of the composite surface after 480 min for both Congo red molecules and Cr(VI) ions.					
30593802	10	34	theme	realistic	1497:1505	arg1	samples					1513:1519	realistic water samples	1497:1519	realistic water samples which make it one of the promising adsorbents	1497:1565	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	5	35	theme	chemisorption	739:751	arg1	nature					753:758	chemisorption nature	739:758	chemisorption nature	739:758	Additionally, the systems are of chemisorption nature and showed excellent fitting with Pseudo-second order model.					
30593802	10	36	theme	adsorbents	1556:1565	arg1	one					1535:1537	one	1535:1537	one	1535:1537	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	10	36	theme	adsorbents	1556:1565	arg1	adsorbents					1556:1565	the promising adsorbents	1542:1565	the promising adsorbents	1542:1565	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	10	37	used	used	1473:1476	arg2	composite					1459:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	9	38	from	water	1407:1411	arg1	molecules					1376:1384	Congo red molecules	1366:1384	Congo red molecules	1366:1384	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	9	38	from	water	1407:1411	arg1	ions					1397:1400	ions	1397:1400	Cr(VI) ions from water	1390:1411	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	9	38	from	water	1407:1411	arg1	reduction					1353:1361	the photocatalytic reduction	1334:1361	the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water	1334:1411	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	9	39	dep	ions	1397:1400	arg1	VI					1393:1394	VI	1393:1394	VI	1393:1394	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	2	40	theme	FT-IR	444:448	arg1	analysis					450:457	FT-IR analysis	444:457	FT-IR analysis	444:457	The formation of the composite was confirmed by the XRD patterns, SEM, HRTEM images, and FT-IR analysis.					
30593802	0	41	theme	@	39:39	arg1	Co					70:71	BE/CH@Co	64:71	BE/CH@Co	64:71	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	41	theme	@	39:39	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	41	theme	@	39:39	arg1	composite					53:61	bentonite/chitosan@cobalt oxide composite	21:61	bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	21:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	1	42	theme	physicochemical	271:285	arg1	properties					287:296	its physicochemical properties	267:296	its physicochemical properties	267:296	Bentonite/chitosan composite supported by green fabricated Co3O4 was successfully synthesized and its physicochemical properties were investigated utilizing several analytic techniques.					
30593802	0	43	from	water	162:166	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	43	from	water	162:166	arg1	Co					70:71	BE/CH@Co	64:71	BE/CH@Co	64:71	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	43	from	water	162:166	arg1	composite					53:61	bentonite/chitosan@cobalt oxide composite	21:61	bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	21:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	8	44	theme	ions	1264:1267	arg1	decontamination					1214:1228	decontamination	1214:1228	decontamination of Congo red molecules and Cr(VI) ions	1214:1267	The composite is of high reusability and can be used effectively for six runs of decontamination of Congo red molecules and Cr(VI) ions.					
30593802	0	45	theme	oxide	47:51	arg1	Co					70:71	BE/CH@Co	64:71	BE/CH@Co	64:71	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	45	theme	oxide	47:51	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	45	theme	oxide	47:51	arg1	composite					53:61	bentonite/chitosan@cobalt oxide composite	21:61	bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	21:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	10	46	theme	samples	1513:1519	arg1	purification					1481:1492	purification	1481:1492	purification of realistic water samples which make it one of the promising adsorbents	1481:1565	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	1	47	theme	Bentonite/chitosan	169:186	arg1	composite					188:196	Bentonite/chitosan composite	169:196	Bentonite/chitosan composite supported by green fabricated Co3O4	169:232	Bentonite/chitosan composite supported by green fabricated Co3O4 was successfully synthesized and its physicochemical properties were investigated utilizing several analytic techniques.					
30593802	3	48	theme	bentonite/chitosan	489:506	arg1	Co3O4					508:512	bentonite/chitosan@Co3O4	489:512	bentonite/chitosan@Co3O4 for acidic dye (Congo red)	489:539	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions were investigated.					
30593802	6	49	theme	isotherm	856:863	arg1	models					865:870	the isotherm models	852:870	the isotherm models	852:870	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	0	50	theme	Cr	149:150	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	1	51	theme	green	211:215	arg1	Co3O4					228:232	green fabricated Co3O4	211:232	green fabricated Co3O4	211:232	Bentonite/chitosan composite supported by green fabricated Co3O4 was successfully synthesized and its physicochemical properties were investigated utilizing several analytic techniques.					
30593802	3	52	theme	acidic	518:523	arg1	dye					525:527	acidic dye	518:527	acidic dye (Congo red)	518:539	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions were investigated.					
30593802	6	53	theme	mathematical	825:836	arg1	parameters					838:847	The mathematical parameters	821:847	The mathematical parameters of the isotherm models	821:870	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	9	54	from	reduction	1353:1361	arg1	water					1407:1411	water	1407:1411	water	1407:1411	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	7	55	theme	maximum	1012:1018	arg1	qmax					1041:1044	qmax	1041:1044	qmax	1041:1044	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	55	theme	maximum	1012:1018	arg1	303 mg/g					1050:1057	303 mg/g	1050:1057	303 mg/g	1050:1057	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	55	theme	maximum	1012:1018	arg1	capacity					1031:1038	The theoretical calculated maximum adsorption capacity	985:1038	The theoretical calculated maximum adsorption capacity (qmax)	985:1045	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	3	56	theme	Co3O4	508:512	arg1	properties					475:484	The adsorption properties	460:484	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions	460:555	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions were investigated.					
30593802	5	57	theme	Pseudo-second	794:806	arg1	model					814:818	Pseudo-second order model	794:818	Pseudo-second order model	794:818	Additionally, the systems are of chemisorption nature and showed excellent fitting with Pseudo-second order model.					
30593802	8	58	dep	Cr	1257:1258	arg1	VI					1260:1261	VI	1260:1261	VI	1260:1261	The composite is of high reusability and can be used effectively for six runs of decontamination of Congo red molecules and Cr(VI) ions.					
30593802	7	59	theme	metal	1107:1111	arg1	ions					1113:1116	metal ions	1107:1116	metal ions	1107:1116	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	60	theme	theoretical	989:999	arg1	qmax					1041:1044	qmax	1041:1044	qmax	1041:1044	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	60	theme	theoretical	989:999	arg1	303 mg/g					1050:1057	303 mg/g	1050:1057	303 mg/g	1050:1057	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	60	theme	theoretical	989:999	arg1	capacity					1031:1038	The theoretical calculated maximum adsorption capacity	985:1038	The theoretical calculated maximum adsorption capacity (qmax)	985:1045	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	9	61	theme	high	1289:1292	arg1	properties					1304:1313	high oxidation properties	1289:1313	high oxidation properties	1289:1313	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	6	62	theme	red	909:911	arg1	molecules					913:921	Congo red molecules	903:921	Congo red molecules	903:921	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	0	63	theme	advanced	101:108	arg1	oxidation					110:118	advanced oxidation	101:118	advanced oxidation	101:118	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	10	64	theme	Co3O4	1447:1451	arg1	composite					1459:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	0	65	theme	removal	120:126	arg1	Co					70:71	BE/CH@Co	64:71	BE/CH@Co	64:71	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	65	theme	removal	120:126	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	65	theme	removal	120:126	arg1	composite					53:61	bentonite/chitosan@cobalt oxide composite	21:61	bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	21:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	5	66	theme	order	808:812	arg1	model					814:818	Pseudo-second order model	794:818	Pseudo-second order model	794:818	Additionally, the systems are of chemisorption nature and showed excellent fitting with Pseudo-second order model.					
30593802	0	67	dep	Cr	149:150	arg1	VI					153:154	VI	153:154	VI	153:154	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	6	68	theme	monolayer	883:891	arg1	uptake					893:898	a monolayer uptake	881:898	a monolayer uptake of Congo red molecules and Cr(VI) ions	881:937	The mathematical parameters of the isotherm models revealed a monolayer uptake of Congo red molecules and Cr(VI) ions and represented mainly by the Langmuir model.					
30593802	4	69	theme	kinetic	580:586	arg1	studies					588:594	The kinetic studies	576:594	The kinetic studies	576:594	The kinetic studies reflected the saturation of the composite surface after 480 min for both Congo red molecules and Cr(VI) ions.					
30593802	10	70	theme	bentonite/chitosan	1428:1445	arg1	composite					1459:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	0	71	theme	Congo	131:135	arg1	dye					141:143	Congo red dye	131:143	Congo red dye	131:143	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	4	72	dep	Cr	693:694	arg1	VI					696:697	VI	696:697	VI	696:697	The kinetic studies reflected the saturation of the composite surface after 480 min for both Congo red molecules and Cr(VI) ions.					
30593802	0	73	theme	dye	141:143	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	74	theme	BE/CH	64:68	arg1	Co					70:71	BE/CH@Co	64:71	BE/CH@Co	64:71	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	74	theme	BE/CH	64:68	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	74	theme	BE/CH	64:68	arg1	composite					53:61	bentonite/chitosan@cobalt oxide composite	21:61	bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	21:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	1	75	theme	several	326:332	arg1	techniques					343:352	several analytic techniques	326:352	several analytic techniques	326:352	Bentonite/chitosan composite supported by green fabricated Co3O4 was successfully synthesized and its physicochemical properties were investigated utilizing several analytic techniques.					
30593802	4	76	theme	surface	638:644	arg1	saturation					610:619	the saturation	606:619	the saturation of the composite surface after 480 min for both Congo red molecules and Cr(VI) ions	606:703	The kinetic studies reflected the saturation of the composite surface after 480 min for both Congo red molecules and Cr(VI) ions.					
30593802	9	77	theme	molecules	1376:1384	arg1	reduction					1353:1361	the photocatalytic reduction	1334:1361	the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water	1334:1411	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	9	78	used	used	1326:1329	arg2	it					1280:1281	it	1280:1281	it	1280:1281	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	0	79	from	composite	53:61	arg1	water					162:166	water	162:166	water	162:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	80	theme	adsorption	86:95	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	9	81	theme	Cr	1390:1391	arg1	ions					1397:1400	ions	1397:1400	Cr(VI) ions from water	1390:1411	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	7	82	theme	red	1082:1084	arg1	molecules					1086:1094	Congo red molecules	1076:1094	Congo red molecules	1076:1094	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	4	83	theme	red	675:677	arg1	molecules					679:687	both Congo red molecules	664:687	both Congo red molecules	664:687	The kinetic studies reflected the saturation of the composite surface after 480 min for both Congo red molecules and Cr(VI) ions.					
30593802	10	84	theme	green	1453:1457	arg1	composite					1459:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	bentonite/chitosan@Co3O4 green composite	1428:1467	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	0	85	theme	bentonite/chitosan	21:38	arg1	Co					70:71	BE/CH@Co	64:71	BE/CH@Co	64:71	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	85	theme	bentonite/chitosan	21:38	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	85	theme	bentonite/chitosan	21:38	arg1	composite					53:61	bentonite/chitosan@cobalt oxide composite	21:61	bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	21:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	9	86	theme	ions	1397:1400	arg1	reduction					1353:1361	the photocatalytic reduction	1334:1361	the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water	1334:1411	Moreover, it is of high oxidation properties and can be used in the photocatalytic reduction of Congo red molecules and Cr(VI) ions from water.					
30593802	2	87	theme	XRD	407:409	arg1	patterns					411:418	the XRD patterns	403:418	the XRD patterns	403:418	The formation of the composite was confirmed by the XRD patterns, SEM, HRTEM images, and FT-IR analysis.					
30593802	0	88	theme	cobalt	40:45	arg1	Co					70:71	BE/CH@Co	64:71	BE/CH@Co	64:71	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	88	theme	cobalt	40:45	arg1	removal					120:126	enhanced adsorption and advanced oxidation removal	77:126	enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	77:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	88	theme	cobalt	40:45	arg1	composite					53:61	bentonite/chitosan@cobalt oxide composite	21:61	bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water	21:166	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	0	89	theme	composite	53:61	arg1	fabrication					6:16	Green fabrication	0:16	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.	0:167	Green fabrication of bentonite/chitosan@cobalt oxide composite (BE/CH@Co) of enhanced adsorption and advanced oxidation removal of Congo red dye and Cr (VI) from water.					
30593802	7	90	dep	Cr	1100:1101	arg1	VI					1103:1104	VI	1103:1104	VI	1103:1104	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	7	90	dep	Cr	1100:1101	arg1	ions					1113:1116	metal ions	1107:1116	metal ions	1107:1116	The theoretical calculated maximum adsorption capacity (qmax) is 303 mg/g and 250 mg/g for Congo red molecules and Cr(VI) metal ions, respectively.					
30593802	3	91	theme	adsorption	464:473	arg1	properties					475:484	The adsorption properties	460:484	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions	460:555	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions were investigated.					
30593802	10	92	theme	water	1507:1511	arg1	samples					1513:1519	realistic water samples	1497:1519	realistic water samples which make it one of the promising adsorbents	1497:1565	Besides that, bentonite/chitosan@Co3O4 green composite was used in purification of realistic water samples which make it one of the promising adsorbents.					
30593802	8	93	theme	high	1153:1156	arg1	reusability					1158:1168	high reusability	1153:1168	high reusability	1153:1168	The composite is of high reusability and can be used effectively for six runs of decontamination of Congo red molecules and Cr(VI) ions.					
30593802	3	94	theme	@	507:507	arg1	Co3O4					508:512	bentonite/chitosan@Co3O4	489:512	bentonite/chitosan@Co3O4 for acidic dye (Congo red)	489:539	The adsorption properties of bentonite/chitosan@Co3O4 for acidic dye (Congo red) and Cr(VI) ions were investigated.					
31635003	5	0	theme	pollutant	760:768	arg1	values					786:791	pollutant and temperature values	760:791	pollutant and temperature values	760:791	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	11	1	theme	alternative	1710:1720	arg1	reuse					1722:1726	the alternative reuse	1706:1726	the alternative reuse of olive pomace	1706:1742	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
31635003	6	2	from	pH 8	911:914	arg1	maximum					900:906	a maximum	898:906	a maximum at pH 8	898:914	The results showed that the adsorption process improved increasing the pH values, with a maximum at pH 8, and it was negatively affected by the presence of salts that retarded the adsorption.					
31635003	4	3	theme	environmental	518:530	arg1	applications					532:543	environmental applications	518:543	environmental applications avoiding the dispersion of the pomace blocked inside the chitosan film	518:614	However, chitosan/olive pomace is proposed as suitable for environmental applications avoiding the dispersion of the pomace blocked inside the chitosan film.					
31635003	1	4	from	water	150:154	arg1	removal					112:118	the removal	108:118	the removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films	108:195	This paper focuses on the removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	9	5	theme	endothermic	1469:1479	arg1	ΔH° > 0					1492:1498	ΔH° > 0	1492:1498	ΔH° > 0	1492:1498	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	5	theme	endothermic	1469:1479	arg1	character					1481:1489	and an endothermic character	1462:1489	character	1481:1489	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	8	6	theme	fundamental	1117:1127	arg1	approach					1107:1114	a low-cost and cleaner approach	1084:1114	a low-cost and cleaner approach	1084:1114	So, a low-cost and cleaner approach, fundamental for the pollutant recovery and for an adsorbent safe reuse, for several cycles of adsorption/desorption, transforming a waste in resource is presented.					
31635003	5	7	theme	several	665:671	arg1	values					712:717	the pH values	705:717	the pH values	705:717	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	7	theme	several	665:671	arg1	adsorbent					746:754	adsorbent	746:754	adsorbent	746:754	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	7	theme	several	665:671	arg1	parameters					686:695	several experimental parameters	665:695	several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values	665:791	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	7	theme	several	665:671	arg1	strength					726:733	ionic strength	720:733	ionic strength	720:733	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	7	theme	several	665:671	arg1	amount					736:741	amount	736:741	amount of adsorbent	736:754	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	7	theme	several	665:671	arg1	values					786:791	pollutant and temperature values	760:791	pollutant and temperature values	760:791	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	11	8	theme	contaminants	1855:1866	arg1	removal/recover					1827:1841	the removal/recover	1823:1841	the removal/recover of emerging contaminants from water	1823:1877	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
31635003	2	9	theme	olive	268:272	arg1	composite					296:304	the olive solid wastes/chitosan composite	264:304	the olive solid wastes/chitosan composite	264:304	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	6	10	theme	salts	967:971	arg1	presence					955:962	the presence	951:962	the presence of salts that retarded the adsorption	951:1000	The results showed that the adsorption process improved increasing the pH values, with a maximum at pH 8, and it was negatively affected by the presence of salts that retarded the adsorption.					
31635003	3	11	theme	Adsorption	347:356	arg1	values					369:374	Adsorption capacities values	347:374	Adsorption capacities values of 16 mg × g-1 and 1.6 mg × g-1	347:406	Adsorption capacities values of 16 mg × g-1 and 1.6 mg × g-1 were obtained for the two adsorbents respectively.					
31635003	5	12	theme	experimental	673:684	arg1	values					712:717	the pH values	705:717	the pH values	705:717	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	12	theme	experimental	673:684	arg1	adsorbent					746:754	adsorbent	746:754	adsorbent	746:754	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	12	theme	experimental	673:684	arg1	parameters					686:695	several experimental parameters	665:695	several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values	665:791	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	12	theme	experimental	673:684	arg1	strength					726:733	ionic strength	720:733	ionic strength	720:733	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	12	theme	experimental	673:684	arg1	amount					736:741	amount	736:741	amount of adsorbent	736:754	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	12	theme	experimental	673:684	arg1	values					786:791	pollutant and temperature values	760:791	pollutant and temperature values	760:791	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	10	13	theme	Oxidation	1547:1555	arg1	Processes					1557:1565	Advanced Oxidation Processes	1538:1565	Advanced Oxidation Processes	1538:1565	The use of Advanced Oxidation Processes was proposed as possible alternative to the tetracycline recovery, obtaining its degradation after the desorption.					
31635003	4	14	theme	chitosan	602:609	arg1	film					611:614	the chitosan film	598:614	the chitosan film	598:614	However, chitosan/olive pomace is proposed as suitable for environmental applications avoiding the dispersion of the pomace blocked inside the chitosan film.					
31635003	4	15	theme	chitosan/olive	468:481	arg1	pomace					483:488	chitosan/olive pomace	468:488	chitosan/olive pomace	468:488	However, chitosan/olive pomace is proposed as suitable for environmental applications avoiding the dispersion of the pomace blocked inside the chitosan film.					
31635003	5	16	theme	adsorption	631:640	arg1	process					642:648	the adsorption process	627:648	the adsorption process	627:648	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	1	17	theme	chitosan-olive	159:172	arg1	films					191:195	chitosan-olive pomace adsorbing films	159:195	chitosan-olive pomace adsorbing films	159:195	This paper focuses on the removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	8	18	theme	safe	1177:1180	arg1	reuse					1182:1186	an adsorbent safe reuse	1164:1186	an adsorbent safe reuse	1164:1186	So, a low-cost and cleaner approach, fundamental for the pollutant recovery and for an adsorbent safe reuse, for several cycles of adsorption/desorption, transforming a waste in resource is presented.					
31635003	0	19	from	water	38:42	arg1	Removal					0:6	Removal	0:6	Removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.	0:84	Removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	8	20	theme	adsorbent	1167:1175	arg1	reuse					1182:1186	an adsorbent safe reuse	1164:1186	an adsorbent safe reuse	1164:1186	So, a low-cost and cleaner approach, fundamental for the pollutant recovery and for an adsorbent safe reuse, for several cycles of adsorption/desorption, transforming a waste in resource is presented.					
31635003	8	21	theme	pollutant	1137:1145	arg1	recovery					1147:1154	the pollutant recovery	1133:1154	the pollutant recovery	1133:1154	So, a low-cost and cleaner approach, fundamental for the pollutant recovery and for an adsorbent safe reuse, for several cycles of adsorption/desorption, transforming a waste in resource is presented.					
31635003	1	22	theme	pomace	174:179	arg1	films					191:195	chitosan-olive pomace adsorbing films	159:195	chitosan-olive pomace adsorbing films	159:195	This paper focuses on the removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	9	23	dep	parameters	1348:1357	arg1	ΔH°					1365:1367	ΔH°	1365:1367	ΔH°	1365:1367	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	23	dep	parameters	1348:1357	arg1	ΔG°					1360:1362	ΔG°	1360:1362	ΔG°	1360:1362	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	23	dep	parameters	1348:1357	arg1	parameters					1348:1357	the thermodynamic parameters	1330:1357	the thermodynamic parameters (ΔG°, ΔH° and ΔS°)	1330:1376	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	23	dep	parameters	1348:1357	arg1	ΔS°					1373:1375	ΔS°	1373:1375	ΔS°	1373:1375	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	8	24	theme	cleaner	1099:1105	arg1	approach					1107:1114	a low-cost and cleaner approach	1084:1114	a low-cost and cleaner approach	1084:1114	So, a low-cost and cleaner approach, fundamental for the pollutant recovery and for an adsorbent safe reuse, for several cycles of adsorption/desorption, transforming a waste in resource is presented.					
31635003	5	25	theme	adsorbent	746:754	arg1	amount					736:741	amount	736:741	amount of adsorbent	736:754	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	25	theme	adsorbent	746:754	arg1	values					712:717	the pH values	705:717	the pH values	705:717	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	25	theme	adsorbent	746:754	arg1	strength					726:733	ionic strength	720:733	ionic strength	720:733	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	25	theme	adsorbent	746:754	arg1	values					786:791	pollutant and temperature values	760:791	pollutant and temperature values	760:791	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	5	25	theme	adsorbent	746:754	arg1	adsorbent					746:754	adsorbent	746:754	adsorbent	746:754	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	1	26	theme	adsorbing	181:189	arg1	films					191:195	chitosan-olive pomace adsorbing films	159:195	chitosan-olive pomace adsorbing films	159:195	This paper focuses on the removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	3	27	theme	16 mg × g-1	379:389	arg1	values					369:374	Adsorption capacities values	347:374	Adsorption capacities values of 16 mg × g-1 and 1.6 mg × g-1	347:406	Adsorption capacities values of 16 mg × g-1 and 1.6 mg × g-1 were obtained for the two adsorbents respectively.					
31635003	10	28	theme	Advanced	1538:1545	arg1	Processes					1557:1565	Advanced Oxidation Processes	1538:1565	Advanced Oxidation Processes	1538:1565	The use of Advanced Oxidation Processes was proposed as possible alternative to the tetracycline recovery, obtaining its degradation after the desorption.					
31635003	7	29	theme	MgCl2	1060:1064	arg1	solution					1070:1077	a MgCl2 2 M solution	1058:1077	a MgCl2 2 M solution	1058:1077	Indeed, the desorption of tetracycline was obtained in a MgCl2 2 M solution.					
31635003	2	30	used	used	324:327	arg2	pomace					252:257	olive pomace	246:257	olive pomace	246:257	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	2	30	used	used	324:327	arg2	wastes					238:243	raw olive solid wastes	222:243	raw olive solid wastes (olive pomace)	222:258	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	7	31	theme	2 M	1066:1068	arg1	solution					1070:1077	a MgCl2 2 M solution	1058:1077	a MgCl2 2 M solution	1058:1077	Indeed, the desorption of tetracycline was obtained in a MgCl2 2 M solution.					
31635003	8	32	theme	several	1193:1199	arg1	cycles					1201:1206	several cycles	1193:1206	several cycles of adsorption/desorption	1193:1231	So, a low-cost and cleaner approach, fundamental for the pollutant recovery and for an adsorbent safe reuse, for several cycles of adsorption/desorption, transforming a waste in resource is presented.					
31635003	3	33	theme	capacities	358:367	arg1	values					369:374	Adsorption capacities values	347:374	Adsorption capacities values of 16 mg × g-1 and 1.6 mg × g-1	347:406	Adsorption capacities values of 16 mg × g-1 and 1.6 mg × g-1 were obtained for the two adsorbents respectively.					
31635003	5	34	theme	pH	709:710	arg1	values					712:717	the pH values	705:717	the pH values	705:717	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	2	35	theme	wastes/chitosan	280:294	arg1	composite					296:304	the olive solid wastes/chitosan composite	264:304	the olive solid wastes/chitosan composite	264:304	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	8	36	theme	adsorption/desorption	1211:1231	arg1	cycles					1201:1206	several cycles	1193:1206	several cycles of adsorption/desorption	1193:1231	So, a low-cost and cleaner approach, fundamental for the pollutant recovery and for an adsorbent safe reuse, for several cycles of adsorption/desorption, transforming a waste in resource is presented.					
31635003	0	37	theme	tetracycline	11:22	arg1	Removal					0:6	Removal	0:6	Removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.	0:84	Removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	9	38	theme	isotherms	1295:1303	arg1	models					1305:1310	isotherms models	1295:1310	isotherms models of adsorption	1295:1324	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	6	39	theme	pH	882:883	arg1	values					885:890	the pH values	878:890	the pH values	878:890	The results showed that the adsorption process improved increasing the pH values, with a maximum at pH 8, and it was negatively affected by the presence of salts that retarded the adsorption.					
31635003	2	40	theme	solid	274:278	arg1	composite					296:304	the olive solid wastes/chitosan composite	264:304	the olive solid wastes/chitosan composite	264:304	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	10	41	theme	tetracycline	1611:1622	arg1	recovery					1624:1631	the tetracycline recovery	1607:1631	the tetracycline recovery	1607:1631	The use of Advanced Oxidation Processes was proposed as possible alternative to the tetracycline recovery, obtaining its degradation after the desorption.					
31635003	5	42	theme	temperature	774:784	arg1	values					786:791	pollutant and temperature values	760:791	pollutant and temperature values	760:791	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	4	43	theme	pomace	576:581	arg1	dispersion					558:567	the dispersion	554:567	the dispersion of the pomace blocked inside the chitosan film	554:614	However, chitosan/olive pomace is proposed as suitable for environmental applications avoiding the dispersion of the pomace blocked inside the chitosan film.					
31635003	7	44	theme	tetracycline	1029:1040	arg1	desorption					1015:1024	the desorption	1011:1024	the desorption of tetracycline	1011:1040	Indeed, the desorption of tetracycline was obtained in a MgCl2 2 M solution.					
31635003	2	45	theme	solid	232:236	arg1	wastes					238:243	raw olive solid wastes	222:243	raw olive solid wastes (olive pomace)	222:258	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	2	45	theme	solid	232:236	arg1	pomace					252:257	olive pomace	246:257	olive pomace	246:257	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	0	46	theme	polluted	29:36	arg1	water					38:42	polluted water	29:42	polluted water	29:42	Removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	5	47	theme	ionic	720:724	arg1	strength					726:733	ionic strength	720:733	ionic strength	720:733	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	2	48	theme	olive	226:230	arg1	wastes					238:243	raw olive solid wastes	222:243	raw olive solid wastes (olive pomace)	222:258	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	2	48	theme	olive	226:230	arg1	pomace					252:257	olive pomace	246:257	olive pomace	246:257	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	11	49	theme	pomace	1737:1742	arg1	reuse					1722:1726	the alternative reuse	1706:1726	the alternative reuse of olive pomace	1706:1742	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
31635003	0	50	theme	chitosan-olive	47:60	arg1	films					79:83	chitosan-olive pomace adsorbing films	47:83	chitosan-olive pomace adsorbing films	47:83	Removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	9	51	theme	adsorption	1315:1324	arg1	ΔG°					1360:1362	ΔG°	1360:1362	ΔG°	1360:1362	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	51	theme	adsorption	1315:1324	arg1	ΔH°					1365:1367	ΔH°	1365:1367	ΔH°	1365:1367	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	51	theme	adsorption	1315:1324	arg1	kinetics					1285:1292	The kinetics	1281:1292	The kinetics	1281:1292	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	51	theme	adsorption	1315:1324	arg1	ΔS°					1373:1375	ΔS°	1373:1375	ΔS°	1373:1375	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	51	theme	adsorption	1315:1324	arg1	models					1305:1310	isotherms models	1295:1310	isotherms models of adsorption	1295:1324	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	51	theme	adsorption	1315:1324	arg1	parameters					1348:1357	the thermodynamic parameters	1330:1357	the thermodynamic parameters (ΔG°, ΔH° and ΔS°)	1330:1376	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	5	52	theme	parameters	686:695	arg1	effect					655:660	the effect	651:660	the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values	651:791	To detail the adsorption process, the effect of several experimental parameters such as the pH values, ionic strength, amount of adsorbent and pollutant and temperature values was investigated.					
31635003	8	53	theme	low-cost	1086:1093	arg1	approach					1107:1114	a low-cost and cleaner approach	1084:1114	a low-cost and cleaner approach	1084:1114	So, a low-cost and cleaner approach, fundamental for the pollutant recovery and for an adsorbent safe reuse, for several cycles of adsorption/desorption, transforming a waste in resource is presented.					
31635003	2	54	theme	raw	222:224	arg1	wastes					238:243	raw olive solid wastes	222:243	raw olive solid wastes (olive pomace)	222:258	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	2	54	theme	raw	222:224	arg1	pomace					252:257	olive pomace	246:257	olive pomace	246:257	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	11	55	from	water	1873:1877	arg1	removal/recover					1827:1841	the removal/recover	1823:1841	the removal/recover of emerging contaminants from water	1823:1877	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
31635003	11	55	from	water	1873:1877	arg1	contaminants					1855:1866	emerging contaminants	1846:1866	emerging contaminants from water	1846:1877	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
31635003	3	56	theme	1.6 mg × g-1	395:406	arg1	values					369:374	Adsorption capacities values	347:374	Adsorption capacities values of 16 mg × g-1 and 1.6 mg × g-1	347:406	Adsorption capacities values of 16 mg × g-1 and 1.6 mg × g-1 were obtained for the two adsorbents respectively.					
31635003	6	57	theme	adsorption	839:848	arg1	process					850:856	the adsorption process	835:856	the adsorption process	835:856	The results showed that the adsorption process improved increasing the pH values, with a maximum at pH 8, and it was negatively affected by the presence of salts that retarded the adsorption.					
31635003	11	58	from	potential	1810:1818	arg1	removal/recover					1827:1841	the removal/recover	1823:1841	the removal/recover of emerging contaminants from water	1823:1877	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
31635003	9	59	theme	pollutant	1438:1446	arg1	physisorption					1417:1429	the physisorption	1413:1429	the physisorption of the pollutant	1413:1446	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	60	theme	thermodynamic	1334:1346	arg1	ΔH°					1365:1367	ΔH°	1365:1367	ΔH°	1365:1367	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	60	theme	thermodynamic	1334:1346	arg1	ΔG°					1360:1362	ΔG°	1360:1362	ΔG°	1360:1362	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	60	theme	thermodynamic	1334:1346	arg1	parameters					1348:1357	the thermodynamic parameters	1330:1357	the thermodynamic parameters (ΔG°, ΔH° and ΔS°)	1330:1376	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	9	60	theme	thermodynamic	1334:1346	arg1	ΔS°					1373:1375	ΔS°	1373:1375	ΔS°	1373:1375	The kinetics, isotherms models of adsorption and the thermodynamic parameters (ΔG°, ΔH° and ΔS°) were also evaluated observing that the physisorption of the pollutant occurred with and an endothermic character (ΔH° > 0) with ΔG° < 0 and ΔS° > 0.					
31635003	11	61	theme	olive	1731:1735	arg1	pomace					1737:1742	olive pomace	1731:1742	olive pomace	1731:1742	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
31635003	2	62	theme	olive	246:250	arg1	wastes					238:243	raw olive solid wastes	222:243	raw olive solid wastes (olive pomace)	222:258	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	2	62	theme	olive	246:250	arg1	pomace					252:257	olive pomace	246:257	olive pomace	246:257	More specifically, both raw olive solid wastes (olive pomace) and the olive solid wastes/chitosan composite were compared and used for this purpose.					
31635003	11	63	from	removal/recover	1827:1841	arg1	water					1873:1877	water	1873:1877	water	1873:1877	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
31635003	1	64	theme	tetracycline	123:134	arg1	removal					112:118	the removal	108:118	the removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films	108:195	This paper focuses on the removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	0	65	theme	adsorbing	69:77	arg1	films					79:83	chitosan-olive pomace adsorbing films	47:83	chitosan-olive pomace adsorbing films	47:83	Removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	11	66	theme	present	1691:1697	arg1	paper					1699:1703	the present paper	1687:1703	the present paper	1687:1703	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
31635003	10	67	theme	possible	1583:1590	arg1	use					1531:1533	The use	1527:1533	The use of Advanced Oxidation Processes	1527:1565	The use of Advanced Oxidation Processes was proposed as possible alternative to the tetracycline recovery, obtaining its degradation after the desorption.					
31635003	10	67	theme	possible	1583:1590	arg1	alternative					1592:1602	possible alternative	1583:1602	possible alternative to the tetracycline recovery	1583:1631	The use of Advanced Oxidation Processes was proposed as possible alternative to the tetracycline recovery, obtaining its degradation after the desorption.					
31635003	0	68	theme	pomace	62:67	arg1	films					79:83	chitosan-olive pomace adsorbing films	47:83	chitosan-olive pomace adsorbing films	47:83	Removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	1	69	theme	polluted	141:148	arg1	water					150:154	polluted water	141:154	polluted water	141:154	This paper focuses on the removal of tetracycline from polluted water by chitosan-olive pomace adsorbing films.					
31635003	10	70	theme	Processes	1557:1565	arg1	use					1531:1533	The use	1527:1533	The use of Advanced Oxidation Processes	1527:1565	The use of Advanced Oxidation Processes was proposed as possible alternative to the tetracycline recovery, obtaining its degradation after the desorption.					
31635003	10	70	theme	Processes	1557:1565	arg1	alternative					1592:1602	possible alternative	1583:1602	possible alternative to the tetracycline recovery	1583:1631	The use of Advanced Oxidation Processes was proposed as possible alternative to the tetracycline recovery, obtaining its degradation after the desorption.					
31635003	11	71	theme	emerging	1846:1853	arg1	contaminants					1855:1866	emerging contaminants	1846:1866	emerging contaminants from water	1846:1877	With the present paper, the alternative reuse of olive pomace is reported avoiding its disposal in the environment claiming its potential in the removal/recover of emerging contaminants from water.					
29986260	2	0	theme	bone	495:498	arg1	infection					500:508	bone infection	495:508	bone infection	495:508	Herein, we proposed a strategy of preparing pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection.					
29986260	8	1	theme	loading	1083:1089	arg1	DLE					1103:1105	DLE	1103:1105	DLE	1103:1105	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	1	theme	loading	1083:1089	arg1	efficiency					1091:1100	drug loading efficiency	1078:1100	drug loading efficiency (DLE) of 92.96%	1078:1116	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	9	2	theme	SA	1203:1204	arg1	MSs					1212:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	3	3	theme	Tea	511:513	arg1	polyphenol					515:524	Tea polyphenol	511:524	Tea polyphenol (TP)	511:529	Tea polyphenol (TP) were encapsulated by porous silica nanospheres (SiO2 NSs).					
29986260	3	3	theme	Tea	511:513	arg1	TP					527:528	TP	527:528	TP	527:528	Tea polyphenol (TP) were encapsulated by porous silica nanospheres (SiO2 NSs).					
29986260	8	4	theme	excellent	1033:1041	arg1	properties					1057:1066	excellent drug delivery properties	1033:1066	excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%	1033:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	4	theme	excellent	1033:1041	arg1	content					1135:1141	drug loading content	1122:1141	drug loading content (DLC) of 19.62%	1122:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	4	theme	excellent	1033:1041	arg1	efficiency					1091:1100	drug loading efficiency	1078:1100	drug loading efficiency (DLE) of 92.96%	1078:1116	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	9	5	theme	osteoblasts	1308:1318	arg1	proliferation					1271:1283	proliferation	1271:1283	proliferation	1271:1283	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	9	5	theme	osteoblasts	1308:1318	arg1	differentiation					1289:1303	differentiation	1289:1303	differentiation	1289:1303	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	7	6	theme	oxidative	993:1001	arg1	stress					1003:1008	oxidative stress	993:1008	oxidative stress	993:1008	At the same time, encapsulated TP is released pH sensitively to resist oxidative stress.					
29986260	6	7	theme	microspheres	833:844	arg1	out-layer					806:814	Mineral out-layer	798:814	Mineral out-layer of the composite microspheres	798:844	Mineral out-layer of the composite microspheres is used to neutralize the acidic environment caused by bacterial infection.					
29986260	6	8	theme	composite	823:831	arg1	microspheres					833:844	the composite microspheres	819:844	the composite microspheres	819:844	Mineral out-layer of the composite microspheres is used to neutralize the acidic environment caused by bacterial infection.					
29986260	4	9	theme	NSs	693:695	arg1	NSs					693:695	TP loaded SiO2 NSs	678:695	TP loaded SiO2 NSs	678:695	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	4	9	theme	NSs	693:695	arg1	lot					671:673	a lot	669:673	a lot of TP loaded SiO2 NSs	669:695	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	9	10	theme	TP	1195:1196	arg1	MSs					1212:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	9	11	theme	@	1197:1197	arg1	MSs					1212:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	9	12	theme	SiO2	1198:1201	arg1	MSs					1212:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	8	13	theme	%	1157:1157	arg1	DLE					1103:1105	DLE	1103:1105	DLE	1103:1105	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	13	theme	%	1157:1157	arg1	DLC					1144:1146	DLC	1144:1146	DLC	1144:1146	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	13	theme	%	1157:1157	arg1	content					1135:1141	drug loading content	1122:1141	drug loading content (DLC) of 19.62%	1122:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	13	theme	%	1157:1157	arg1	efficiency					1091:1100	drug loading efficiency	1078:1100	drug loading efficiency (DLE) of 92.96%	1078:1116	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	2	14	dep	responsive	353:362	arg1	multistage					380:389	multistage	380:389	multistage	380:389	Herein, we proposed a strategy of preparing pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection.					
29986260	2	14	dep	responsive	353:362	arg1	antibacterial					365:377	antibacterial	365:377	antibacterial	365:377	Herein, we proposed a strategy of preparing pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection.					
29986260	8	15	theme	%	1116:1116	arg1	DLE					1103:1105	DLE	1103:1105	DLE	1103:1105	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	15	theme	%	1116:1116	arg1	DLC					1144:1146	DLC	1144:1146	DLC	1144:1146	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	15	theme	%	1116:1116	arg1	content					1135:1141	drug loading content	1122:1141	drug loading content (DLC) of 19.62%	1122:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	15	theme	%	1116:1116	arg1	efficiency					1091:1100	drug loading efficiency	1078:1100	drug loading efficiency (DLE) of 92.96%	1078:1116	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	5	16	theme	TP	726:727	arg1	spheres					746:752	obtained TP@SiO2@SA microgel spheres	717:752	obtained TP@SiO2@SA microgel spheres	717:752	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	5	17	theme	SiO2	729:732	arg1	spheres					746:752	obtained TP@SiO2@SA microgel spheres	717:752	obtained TP@SiO2@SA microgel spheres	717:752	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	9	18	theme	@	1202:1202	arg1	MSs					1212:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	8	19	theme	loading	1127:1133	arg1	DLC					1144:1146	DLC	1144:1146	DLC	1144:1146	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	19	theme	loading	1127:1133	arg1	content					1135:1141	drug loading content	1122:1141	drug loading content (DLC) of 19.62%	1122:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	4	20	theme	SA	613:614	arg1	MSs					635:637	MSs	635:637	MSs	635:637	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	4	20	theme	SA	613:614	arg1	spheres					626:632	sodium alginate (SA) microgel spheres	596:632	sodium alginate (SA) microgel spheres (MSs)	596:638	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	0	21	dep	microgels	80:88	arg1	used					90:93	used	90:93	microgels used for minimally invasive treatment of bone infection	80:144	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	1	22	theme	bone	164:167	arg1	infection					169:177	bone infection	164:177	bone infection	164:177	The treatment of bone infection requires drug carriers take large number of cargo, be antibacterial, promote proliferation and differentiation of osteoblasts.					
29986260	5	23	theme	@	733:733	arg1	spheres					746:752	obtained TP@SiO2@SA microgel spheres	717:752	obtained TP@SiO2@SA microgel spheres	717:752	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	6	24	theme	Mineral	798:804	arg1	out-layer					806:814	Mineral out-layer	798:814	Mineral out-layer of the composite microspheres	798:844	Mineral out-layer of the composite microspheres is used to neutralize the acidic environment caused by bacterial infection.					
29986260	9	25	theme	@	1205:1205	arg1	MSs					1212:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	4	26	theme	SiO2	688:691	arg1	NSs					693:695	TP loaded SiO2 NSs	678:695	TP loaded SiO2 NSs	678:695	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	1	27	theme	infection	169:177	arg1	treatment					151:159	The treatment	147:159	The treatment of bone infection	147:177	The treatment of bone infection requires drug carriers take large number of cargo, be antibacterial, promote proliferation and differentiation of osteoblasts.					
29986260	5	28	theme	SA	734:735	arg1	spheres					746:752	obtained TP@SiO2@SA microgel spheres	717:752	obtained TP@SiO2@SA microgel spheres	717:752	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	6	29	theme	bacterial	901:909	arg1	infection					911:919	bacterial infection	901:919	bacterial infection	901:919	Mineral out-layer of the composite microspheres is used to neutralize the acidic environment caused by bacterial infection.					
29986260	4	30	theme	loaded	681:686	arg1	NSs					693:695	TP loaded SiO2 NSs	678:695	TP loaded SiO2 NSs	678:695	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	0	31	theme	tea	23:25	arg1	polyphenol					27:36	green tea polyphenol	17:36	green tea polyphenol	17:36	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	9	32	theme	CaCO3	1206:1210	arg1	MSs					1212:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	TP@SiO2@SA@CaCO3 MSs	1195:1214	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	3	33	theme	porous	552:557	arg1	nanospheres					566:576	porous silica nanospheres	552:576	porous silica nanospheres (SiO2 NSs)	552:587	Tea polyphenol (TP) were encapsulated by porous silica nanospheres (SiO2 NSs).					
29986260	3	33	theme	porous	552:557	arg1	NSs					584:586	NSs	584:586	NSs	584:586	Tea polyphenol (TP) were encapsulated by porous silica nanospheres (SiO2 NSs).					
29986260	1	34	theme	drug	188:191	arg1	carriers					193:200	drug carriers	188:200	drug carriers	188:200	The treatment of bone infection requires drug carriers take large number of cargo, be antibacterial, promote proliferation and differentiation of osteoblasts.					
29986260	2	35	theme	green	433:437	arg1	polyphenol					443:452	green tea polyphenol	433:452	green tea polyphenol used for minimally invasive treatment of bone infection	433:508	Herein, we proposed a strategy of preparing pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection.					
29986260	0	36	theme	invasive	109:116	arg1	treatment					118:126	minimally invasive treatment	99:126	minimally invasive treatment of bone infection	99:144	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	9	37	from	pH = 5.5	1349:1356	arg1	stimulation					1326:1336	stimulation	1326:1336	stimulation of H2O2 at pH = 5.5	1326:1356	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	3	38	theme	silica	559:564	arg1	nanospheres					566:576	porous silica nanospheres	552:576	porous silica nanospheres (SiO2 NSs)	552:587	Tea polyphenol (TP) were encapsulated by porous silica nanospheres (SiO2 NSs).					
29986260	3	38	theme	silica	559:564	arg1	NSs					584:586	NSs	584:586	NSs	584:586	Tea polyphenol (TP) were encapsulated by porous silica nanospheres (SiO2 NSs).					
29986260	5	39	theme	microgel	737:744	arg1	spheres					746:752	obtained TP@SiO2@SA microgel spheres	717:752	obtained TP@SiO2@SA microgel spheres	717:752	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	5	40	theme	obtained	717:724	arg1	spheres					746:752	obtained TP@SiO2@SA microgel spheres	717:752	obtained TP@SiO2@SA microgel spheres	717:752	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	0	41	theme	bone	131:134	arg1	infection					136:144	bone infection	131:144	bone infection	131:144	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	4	42	theme	microgel	617:624	arg1	MSs					635:637	MSs	635:637	MSs	635:637	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	4	42	theme	microgel	617:624	arg1	spheres					626:632	sodium alginate (SA) microgel spheres	596:632	sodium alginate (SA) microgel spheres (MSs)	596:638	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	0	43	theme	polyphenol	27:36	arg1	Encapsulation					0:12	Encapsulation	0:12	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate	0:78	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	1	44	theme	osteoblasts	293:303	arg1	differentiation					274:288	differentiation	274:288	differentiation	274:288	The treatment of bone infection requires drug carriers take large number of cargo, be antibacterial, promote proliferation and differentiation of osteoblasts.					
29986260	1	44	theme	osteoblasts	293:303	arg1	proliferation					256:268	proliferation	256:268	proliferation	256:268	The treatment of bone infection requires drug carriers take large number of cargo, be antibacterial, promote proliferation and differentiation of osteoblasts.					
29986260	2	45	theme	invasive	473:480	arg1	treatment					482:490	minimally invasive treatment	463:490	minimally invasive treatment of bone infection	463:508	Herein, we proposed a strategy of preparing pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection.					
29986260	8	46	theme	drug	1078:1081	arg1	DLE					1103:1105	DLE	1103:1105	DLE	1103:1105	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	46	theme	drug	1078:1081	arg1	efficiency					1091:1100	drug loading efficiency	1078:1100	drug loading efficiency (DLE) of 92.96%	1078:1116	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	2	47	theme	tea	439:441	arg1	polyphenol					443:452	green tea polyphenol	433:452	green tea polyphenol used for minimally invasive treatment of bone infection	433:508	Herein, we proposed a strategy of preparing pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection.					
29986260	2	48	theme	structured	391:400	arg1	microspheres					402:413	pH responsive, antibacterial, multistage structured microspheres	350:413	pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection	350:508	Herein, we proposed a strategy of preparing pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection.					
29986260	5	49	theme	@	728:728	arg1	spheres					746:752	obtained TP@SiO2@SA microgel spheres	717:752	obtained TP@SiO2@SA microgel spheres	717:752	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	6	50	theme	acidic	872:877	arg1	environment					879:889	the acidic environment	868:889	the acidic environment caused by bacterial infection	868:919	Mineral out-layer of the composite microspheres is used to neutralize the acidic environment caused by bacterial infection.					
29986260	8	51	theme	drug	1122:1125	arg1	DLC					1144:1146	DLC	1144:1146	DLC	1144:1146	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	51	theme	drug	1122:1125	arg1	content					1135:1141	drug loading content	1122:1141	drug loading content (DLC) of 19.62%	1122:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	5	52	theme	outer	702:706	arg1	layer					708:712	The outer layer	698:712	The outer layer of obtained TP@SiO2@SA microgel spheres	698:752	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	7	53	theme	encapsulated	940:951	arg1	TP					953:954	encapsulated TP	940:954	encapsulated TP	940:954	At the same time, encapsulated TP is released pH sensitively to resist oxidative stress.					
29986260	0	54	theme	pH	41:42	arg1	alginate					71:78	alginate	71:78	alginate	71:78	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	0	54	theme	pH	41:42	arg1	antibacterial					56:68	antibacterial	56:68	antibacterial	56:68	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	0	54	theme	pH	41:42	arg1	responsive					44:53	pH responsive	41:53	pH responsive	41:53	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	0	55	theme	infection	136:144	arg1	treatment					118:126	minimally invasive treatment	99:126	minimally invasive treatment of bone infection	99:144	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	7	56	theme	same	929:932	arg1	time					934:937	the same time	925:937	the same time	925:937	At the same time, encapsulated TP is released pH sensitively to resist oxidative stress.					
29986260	8	57	theme	delivery	1048:1055	arg1	properties					1057:1066	excellent drug delivery properties	1033:1066	excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%	1033:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	57	theme	delivery	1048:1055	arg1	content					1135:1141	drug loading content	1122:1141	drug loading content (DLC) of 19.62%	1122:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	57	theme	delivery	1048:1055	arg1	efficiency					1091:1100	drug loading efficiency	1078:1100	drug loading efficiency (DLE) of 92.96%	1078:1116	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	1	58	dep	take	202:205	arg1	antibacterial					233:245	antibacterial	233:245	antibacterial	233:245	The treatment of bone infection requires drug carriers take large number of cargo, be antibacterial, promote proliferation and differentiation of osteoblasts.					
29986260	1	58	dep	take	202:205	arg1	promote					248:254	promote	248:254	promote proliferation and differentiation of osteoblasts	248:303	The treatment of bone infection requires drug carriers take large number of cargo, be antibacterial, promote proliferation and differentiation of osteoblasts.					
29986260	8	59	theme	drug	1043:1046	arg1	properties					1057:1066	excellent drug delivery properties	1033:1066	excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%	1033:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	59	theme	drug	1043:1046	arg1	content					1135:1141	drug loading content	1122:1141	drug loading content (DLC) of 19.62%	1122:1157	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	8	59	theme	drug	1043:1046	arg1	efficiency					1091:1100	drug loading efficiency	1078:1100	drug loading efficiency (DLE) of 92.96%	1078:1116	Our results exhibited excellent drug delivery properties including drug loading efficiency (DLE) of 92.96% and drug loading content (DLC) of 19.62%.					
29986260	5	60	theme	spheres	746:752	arg1	layer					708:712	The outer layer	698:712	The outer layer of obtained TP@SiO2@SA microgel spheres	698:752	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	9	61	theme	H2O2	1341:1344	arg1	stimulation					1326:1336	stimulation	1326:1336	stimulation of H2O2 at pH = 5.5	1326:1356	Besides, results demonstrated that TP@SiO2@SA@CaCO3 MSs can effectively kill Staphylococcus aureus and promote proliferation and differentiation of osteoblasts under stimulation of H2O2 at pH = 5.5.					
29986260	1	62	theme	large	207:211	arg1	number					213:218	large number	207:218	large number of cargo	207:227	The treatment of bone infection requires drug carriers take large number of cargo, be antibacterial, promote proliferation and differentiation of osteoblasts.					
29986260	6	63	used	used	849:852	arg2	out-layer					806:814	Mineral out-layer	798:814	Mineral out-layer of the composite microspheres	798:844	Mineral out-layer of the composite microspheres is used to neutralize the acidic environment caused by bacterial infection.					
29986260	4	64	theme	alginate	603:610	arg1	MSs					635:637	MSs	635:637	MSs	635:637	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	4	64	theme	alginate	603:610	arg1	spheres					626:632	sodium alginate (SA) microgel spheres	596:632	sodium alginate (SA) microgel spheres (MSs)	596:638	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	2	65	theme	responsive	353:362	arg1	microspheres					402:413	pH responsive, antibacterial, multistage structured microspheres	350:413	pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection	350:508	Herein, we proposed a strategy of preparing pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection.					
29986260	5	66	theme	sensitive	781:789	arg1	CaCO3					791:795	pH sensitive CaCO3	778:795	pH sensitive CaCO3	778:795	The outer layer of obtained TP@SiO2@SA microgel spheres were further wrapped by pH sensitive CaCO3.					
29986260	1	67	theme	cargo	223:227	arg1	number					213:218	large number	207:218	large number of cargo	207:227	The treatment of bone infection requires drug carriers take large number of cargo, be antibacterial, promote proliferation and differentiation of osteoblasts.					
29986260	2	68	theme	infection	500:508	arg1	treatment					482:490	minimally invasive treatment	463:490	minimally invasive treatment of bone infection	463:508	Herein, we proposed a strategy of preparing pH responsive, antibacterial, multistage structured microspheres encapsulated with green tea polyphenol used for minimally invasive treatment of bone infection.					
29986260	0	69	theme	green	17:21	arg1	polyphenol					27:36	green tea polyphenol	17:36	green tea polyphenol	17:36	Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection.					
29986260	4	70	theme	sodium	596:601	arg1	MSs					635:637	MSs	635:637	MSs	635:637	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29986260	4	70	theme	sodium	596:601	arg1	spheres					626:632	sodium alginate (SA) microgel spheres	596:632	sodium alginate (SA) microgel spheres (MSs)	596:638	Then, sodium alginate (SA) microgel spheres (MSs) were prepared to encapsulate a lot of TP loaded SiO2 NSs.					
29578023	2	0	theme	fatty	320:324	arg1	simulant					326:333	food fatty simulant	315:333	food fatty simulant	315:333	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	7	1	theme	physical	829:836	arg1	mixture					838:844	physical mixture	829:844	physical mixture of β-CD and GA	829:859	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	8	2	theme	water	1085:1089	arg1	WVP					1111:1113	WVP	1111:1113	WVP	1111:1113	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	8	2	theme	water	1085:1089	arg1	permeability					1097:1108	water vapor permeability	1085:1108	water vapor permeability (WVP)	1085:1114	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	10	3	theme	anti-oxidant	1446:1457	arg1	demands					1459:1465	higher anti-oxidant demands	1439:1465	higher anti-oxidant demands	1439:1465	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	2	4	theme	Gallic	281:286	arg1	GA					294:295	GA	294:295	GA	294:295	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	2	4	theme	Gallic	281:286	arg1	acid					288:291	Gallic acid	281:291	Gallic acid (GA) from the film	281:310	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	4	5	theme	physical	632:639	arg1	mixture					641:647	physical mixture	632:647	physical mixture	632:647	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	9	6	with	properties	1241:1250	arg1	ethanol					1174:1180	ethanol	1174:1180	ethanol	1174:1180	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	5	7	theme	GA	689:690	arg1	causes					692:697	GA causes	689:697	GA causes	689:697	Incorporation of GA causes leads to formation of weak and dark samples.					
29578023	9	8	theme	release	1135:1141	arg1	rate					1143:1146	the lowest release rate	1124:1146	the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties	1124:1250	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	6	9	with	Use	744:746	arg1	GA					761:762	GA	761:762	GA	761:762	Use of β-CD with GA decreased tensile strength (TS) significantly.					
29578023	1	10	theme	optical	216:222	arg1	properties					249:258	some optical, physical and mechanical properties	211:258	some optical, physical and mechanical properties	211:258	Chitosan, gelatin and their blended films prepared and compared in some optical, physical and mechanical properties.					
29578023	8	11	from	concentration	1011:1023	arg1	Incorporation					977:989	Incorporation	977:989	Incorporation	977:989	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	7	12	theme	inclusion	958:966	arg1	complex					968:974	their inclusion complex	952:974	their inclusion complex	952:974	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	10	13	contain	contained	1379:1387	arg2	complex					1408:1414	GA- β-CD inclusion complex	1389:1414	GA- β-CD inclusion complex	1389:1414	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	10	13	contain	contained	1379:1387	arg2	rate					1355:1358	highest rate	1347:1358	highest rate related to samples	1347:1377	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	10	13	contain	contained	1379:1387	arg1	hand					1341:1344	the other hand	1331:1344	the other hand	1331:1344	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	10	13	contain	contained	1379:1387	arg1	rate					1355:1358	highest rate	1347:1358	highest rate related to samples	1347:1377	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	7	14	with	comparison	936:945	arg1	complex					968:974	their inclusion complex	952:974	their inclusion complex	952:974	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	5	15	theme	dark	730:733	arg1	samples					735:741	weak and dark samples	721:741	weak and dark samples	721:741	Incorporation of GA causes leads to formation of weak and dark samples.					
29578023	4	16	with	concentration	564:576	arg1	β-CD					623:626	β-CD	623:626	β-CD	623:626	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	4	16	with	concentration	564:576	arg1	ethanol					593:599	ethanol	593:599	ethanol	593:599	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	4	16	with	concentration	564:576	arg1	β-Cyclodextrin					607:620	β-Cyclodextrin	607:620	β-Cyclodextrin (β-CD) in physical mixture and encapsulated form	607:669	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	10	17	theme	other	1335:1339	arg1	hand					1341:1344	the other hand	1331:1344	the other hand	1331:1344	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	7	18	theme	release	920:926	arg1	rate					928:931	release rate	920:931	release rate	920:931	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	0	19	from	Effect	0:5	arg1	properties					46:55	physiochemical properties	31:55	physiochemical properties	31:55	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	0	19	from	Effect	0:5	arg1	release					74:80	anti-oxidant release	61:80	anti-oxidant release from chitosan-gelatin composite films to fatty food simulant	61:141	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	0	20	theme	composite	104:112	arg1	films					114:118	chitosan-gelatin composite films	87:118	chitosan-gelatin composite films	87:118	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	5	21	theme	weak	721:724	arg1	samples					735:741	weak and dark samples	721:741	weak and dark samples	721:741	Incorporation of GA causes leads to formation of weak and dark samples.					
29578023	3	22	theme	gelatin	358:364	arg1	films					372:376	The chitosan- gelatin based films	344:376	The chitosan- gelatin based films	344:376	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	7	23	theme	barrier	877:883	arg1	properties					885:894	lower barrier properties	871:894	lower barrier properties	871:894	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	9	24	dep	good	1200:1203	arg1	desirable					1206:1214	desirable	1206:1214	desirable	1206:1214	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	3	25	theme	singular	455:462	arg1	gelatin					464:470	singular gelatin	455:470	singular gelatin	455:470	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	0	26	theme	organic	10:16	arg1	additives					18:26	organic additives	10:26	organic additives	10:26	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	3	27	theme	appropriate	385:395	arg1	compatibility					397:409	appropriate compatibility	385:409	appropriate compatibility	385:409	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	8	28	theme	release	1041:1047	arg1	rate					1049:1052	higher release rate	1034:1052	higher release rate	1034:1052	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	4	29	theme	different	510:518	arg1	methods					520:526	different methods	510:526	different methods including simple addition	510:552	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	0	30	theme	anti-oxidant	61:72	arg1	release					74:80	anti-oxidant release	61:80	anti-oxidant release from chitosan-gelatin composite films to fatty food simulant	61:141	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	2	31	theme	food	315:318	arg1	simulant					326:333	food fatty simulant	315:333	food fatty simulant	315:333	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	10	32	theme	β-CD	1393:1396	arg1	complex					1408:1414	GA- β-CD inclusion complex	1389:1414	GA- β-CD inclusion complex	1389:1414	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	7	33	theme	lower	871:875	arg1	properties					885:894	lower barrier properties	871:894	lower barrier properties	871:894	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	7	34	with	samples	816:822	arg1	mixture					838:844	physical mixture	829:844	physical mixture of β-CD and GA	829:859	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	7	35	theme	GA	858:859	arg1	mixture					838:844	physical mixture	829:844	physical mixture of β-CD and GA	829:859	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	9	36	theme	fatty	1291:1295	arg1	packaging					1302:1310	fatty food packaging	1291:1310	fatty food packaging	1291:1310	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	5	37	theme	samples	735:741	arg1	formation					708:716	formation	708:716	formation of weak and dark samples	708:741	Incorporation of GA causes leads to formation of weak and dark samples.					
29578023	7	38	theme	β-CD	849:852	arg1	mixture					838:844	physical mixture	829:844	physical mixture of β-CD and GA	829:859	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	2	39	from	film	307:310	arg1	GA					294:295	GA	294:295	GA	294:295	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	2	39	from	film	307:310	arg1	rate					273:276	The release rate	261:276	The release rate of Gallic acid (GA) from the film to food fatty simulant	261:333	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	2	39	from	film	307:310	arg1	acid					288:291	Gallic acid	281:291	Gallic acid (GA) from the film	281:310	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	6	40	theme	tensile	774:780	arg1	TS					792:793	TS	792:793	TS	792:793	Use of β-CD with GA decreased tensile strength (TS) significantly.					
29578023	6	40	theme	tensile	774:780	arg1	strength					782:789	tensile strength	774:789	tensile strength (TS)	774:794	Use of β-CD with GA decreased tensile strength (TS) significantly.					
29578023	2	41	theme	acid	288:291	arg1	rate					273:276	The release rate	261:276	The release rate of Gallic acid (GA) from the film to food fatty simulant	261:333	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	9	42	theme	long	1315:1318	arg1	period					1320:1325	long period	1315:1325	long period	1315:1325	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	4	43	theme	tween	581:585	arg1	concentration					564:576	two concentration	560:576	two concentration of tween	560:585	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	10	44	theme	higher	1439:1444	arg1	demands					1459:1465	higher anti-oxidant demands	1439:1465	higher anti-oxidant demands	1439:1465	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	1	45	theme	blended	172:178	arg1	films					180:184	their blended films	166:184	their blended films	166:184	Chitosan, gelatin and their blended films prepared and compared in some optical, physical and mechanical properties.					
29578023	8	46	theme	vapor	1091:1095	arg1	WVP					1111:1113	WVP	1111:1113	WVP	1111:1113	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	8	46	theme	vapor	1091:1095	arg1	permeability					1097:1108	water vapor permeability	1085:1108	water vapor permeability (WVP)	1085:1114	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	6	47	theme	β-CD	751:754	arg1	Use					744:746	Use	744:746	Use of β-CD with GA	744:762	Use of β-CD with GA decreased tensile strength (TS) significantly.					
29578023	7	48	theme	Film	811:814	arg1	samples					816:822	Film samples	811:822	Film samples with physical mixture of β-CD and GA	811:859	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	10	49	theme	short-term	1471:1480	arg1	protection					1482:1491	short-term protection	1471:1491	short-term protection	1471:1491	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	3	50	with	comparison	439:448	arg1	film					485:488	chitosan film	476:488	chitosan film	476:488	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	3	50	with	comparison	439:448	arg1	gelatin					464:470	singular gelatin	455:470	singular gelatin	455:470	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	9	51	theme	lowest	1128:1133	arg1	rate					1143:1146	the lowest release rate	1124:1146	the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties	1124:1250	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	5	52	theme	causes	692:697	arg1	Incorporation					672:684	Incorporation	672:684	Incorporation of GA causes	672:697	Incorporation of GA causes leads to formation of weak and dark samples.					
29578023	4	53	from	β-Cyclodextrin	607:620	arg1	mixture					641:647	physical mixture	632:647	physical mixture	632:647	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	4	53	from	β-Cyclodextrin	607:620	arg1	form					666:669	encapsulated form	653:669	encapsulated form	653:669	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	9	54	theme	good	1200:1203	arg1	properties					1241:1250	almost good (desirable) physical and mechanical properties	1193:1250	samples with ethanol as well as almost good (desirable) physical and mechanical properties	1161:1250	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	2	55	theme	release	265:271	arg1	rate					273:276	The release rate	261:276	The release rate of Gallic acid (GA) from the film to food fatty simulant	261:333	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	2	56	from	rate	273:276	arg1	film					307:310	the film	303:310	the film	303:310	The release rate of Gallic acid (GA) from the film to food fatty simulant studied.					
29578023	0	57	from	films	114:118	arg1	properties					46:55	physiochemical properties	31:55	physiochemical properties	31:55	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	0	57	from	films	114:118	arg1	release					74:80	anti-oxidant release	61:80	anti-oxidant release from chitosan-gelatin composite films to fatty food simulant	61:141	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	4	58	theme	encapsulated	653:664	arg1	form					666:669	encapsulated form	653:669	encapsulated form	653:669	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	0	59	theme	chitosan-gelatin	87:102	arg1	films					114:118	chitosan-gelatin composite films	87:118	chitosan-gelatin composite films	87:118	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	8	60	theme	tween	994:998	arg1	Incorporation					977:989	Incorporation	977:989	Incorporation	977:989	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	8	61	from	break	1069:1073	arg1	%					1078:1078	EB%	1076:1078	EB%	1076:1078	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	8	61	from	break	1069:1073	arg1	elongation					1055:1064	elongation	1055:1064	elongation at break (EB%)	1055:1079	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	8	61	from	break	1069:1073	arg1	rate					1049:1052	higher release rate	1034:1052	higher release rate	1034:1052	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	8	61	from	break	1069:1073	arg1	WVP					1111:1113	WVP	1111:1113	WVP	1111:1113	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	8	61	from	break	1069:1073	arg1	permeability					1097:1108	water vapor permeability	1085:1108	water vapor permeability (WVP)	1085:1114	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	0	62	theme	food	129:132	arg1	simulant					134:141	fatty food simulant	123:141	fatty food simulant	123:141	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	3	63	from	compatibility	397:409	arg1	comparison					439:448	comparison	439:448	comparison with singular gelatin and chitosan film	439:488	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	0	64	theme	fatty	123:127	arg1	simulant					134:141	fatty food simulant	123:141	fatty food simulant	123:141	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	8	65	theme	low	1007:1009	arg1	concentration					1011:1023	low concentration	1007:1023	low concentration	1007:1023	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	10	66	theme	highest	1347:1353	arg1	rate					1355:1358	highest rate	1347:1358	highest rate related to samples	1347:1377	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	9	67	with	samples	1161:1167	arg1	ethanol					1174:1180	ethanol	1174:1180	ethanol	1174:1180	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	9	68	theme	physical	1217:1224	arg1	properties					1241:1250	almost good (desirable) physical and mechanical properties	1193:1250	samples with ethanol as well as almost good (desirable) physical and mechanical properties	1161:1250	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	7	69	theme	water	904:908	arg1	vapor					910:914	water vapor	904:914	water vapor	904:914	Film samples with physical mixture of β-CD and GA indicated lower barrier properties against water vapor and release rate in comparison with their inclusion complex.					
29578023	3	70	theme	based	366:370	arg1	films					372:376	The chitosan- gelatin based films	344:376	The chitosan- gelatin based films	344:376	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	3	71	theme	desirable	415:423	arg1	properties					425:434	desirable properties	415:434	desirable properties	415:434	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	0	72	theme	additives	18:26	arg1	Effect					0:5	Effect	0:5	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.	0:142	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	9	73	theme	mechanical	1230:1239	arg1	properties					1241:1250	almost good (desirable) physical and mechanical properties	1193:1250	samples with ethanol as well as almost good (desirable) physical and mechanical properties	1161:1250	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	8	74	theme	higher	1034:1039	arg1	rate					1049:1052	higher release rate	1034:1052	higher release rate	1034:1052	Incorporation of tween 20, at low concentration leads to higher release rate, elongation at break (EB%) and water vapor permeability (WVP).					
29578023	3	75	theme	chitosan-	348:356	arg1	films					372:376	The chitosan- gelatin based films	344:376	The chitosan- gelatin based films	344:376	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	0	76	theme	physiochemical	31:44	arg1	properties					46:55	physiochemical properties	31:55	physiochemical properties	31:55	Effect of organic additives on physiochemical properties and anti-oxidant release from chitosan-gelatin composite films to fatty food simulant.					
29578023	10	77	theme	related	1360:1366	arg1	rate					1355:1358	highest rate	1347:1358	highest rate related to samples	1347:1377	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	4	78	theme	simple	538:543	arg1	addition					545:552	simple addition	538:552	simple addition	538:552	GA was added using different methods including simple addition, with two concentration of tween, with ethanol, with β-Cyclodextrin (β-CD) in physical mixture and encapsulated form.					
29578023	10	79	theme	inclusion	1398:1406	arg1	complex					1408:1414	GA- β-CD inclusion complex	1389:1414	GA- β-CD inclusion complex	1389:1414	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	9	80	theme	appropriate	1265:1275	arg1	candidate					1277:1285	appropriate candidate	1265:1285	appropriate candidate for fatty food packaging in long period	1265:1325	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	9	81	from	candidate	1277:1285	arg1	period					1320:1325	long period	1315:1325	long period	1315:1325	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
29578023	10	82	theme	GA-	1389:1391	arg1	complex					1408:1414	GA- β-CD inclusion complex	1389:1414	GA- β-CD inclusion complex	1389:1414	On the other hand, highest rate related to samples contained GA- β-CD inclusion complex, which are suitable to higher anti-oxidant demands and short-term protection.					
29578023	3	83	theme	chitosan	476:483	arg1	film					485:488	chitosan film	476:488	chitosan film	476:488	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	1	84	theme	physical	225:232	arg1	properties					249:258	some optical, physical and mechanical properties	211:258	some optical, physical and mechanical properties	211:258	Chitosan, gelatin and their blended films prepared and compared in some optical, physical and mechanical properties.					
29578023	1	85	theme	mechanical	238:247	arg1	properties					249:258	some optical, physical and mechanical properties	211:258	some optical, physical and mechanical properties	211:258	Chitosan, gelatin and their blended films prepared and compared in some optical, physical and mechanical properties.					
29578023	3	86	from	properties	425:434	arg1	comparison					439:448	comparison	439:448	comparison with singular gelatin and chitosan film	439:488	The chitosan- gelatin based films showed appropriate compatibility and desirable properties in comparison with singular gelatin and chitosan film.					
29578023	9	87	theme	food	1297:1300	arg1	packaging					1302:1310	fatty food packaging	1291:1310	fatty food packaging	1291:1310	Due to the lowest release rate observed for samples with ethanol as well as almost good (desirable) physical and mechanical properties, it could be appropriate candidate for fatty food packaging in long period.					
31752698	11	0	theme	IXB-mediated	1781:1792	arg1	induction					1794:1802	the TA- and IXB-mediated induction	1769:1802	the TA- and IXB-mediated induction of cell death	1769:1816	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures prevented the TA- and IXB-mediated induction of cell death.					
31752698	9	1	theme	-based	1340:1345	arg1	spectroscopy					1353:1364	Atomic Force Microscopy (AFM)-based force spectroscopy	1311:1364	Atomic Force Microscopy (AFM)-based force spectroscopy	1311:1364	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	14	2	theme	wall	2133:2136	arg1	properties					2110:2119	the mechanical properties	2095:2119	the mechanical properties of the cell wall perturbed by CBIs	2095:2154	Auxin treatment alone or with CBI also decreased cell wall stiffness, showing that the mechanical properties of the cell wall perturbed by CBIs were not restored by auxin.					
31752698	3	3	theme	plant	532:536	arg1	tissues					538:544	plant tissues	532:544	plant tissues	532:544	These processes may facilitate S. scabies entry into plant tissues.					
31752698	8	4	from	range	1171:1175	arg1	cells					1197:1201	plant cells	1191:1201	plant cells	1191:1201	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	11	5	theme	cell	1807:1810	arg1	death					1812:1816	cell death	1807:1816	cell death	1807:1816	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures prevented the TA- and IXB-mediated induction of cell death.					
31752698	5	6	theme	death	830:834	arg1	induction					812:820	The induction	808:820	The induction of cell death by TA	808:840	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	6	theme	death	830:834	arg1	RESULTS					800:806	RESULTS	800:806	RESULTS The induction of cell death by TA and IXB	800:848	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	6	theme	death	830:834	arg1	IXB					846:848	IXB	846:848	IXB	846:848	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	12	7	theme	transport	1882:1890	arg1	inhibition					1856:1865	inhibition	1856:1865	inhibition of polar auxin transport during CBI-treatment	1856:1911	Cell survival was also stimulated by inhibition of polar auxin transport during CBI-treatment.					
31752698	9	8	from	IXB	1425:1427	arg1	cells					1452:1456	suspension-cultured cells	1432:1456	suspension-cultured cells	1432:1456	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	4	9	theme	synthesis	652:660	arg1	inhibition					628:637	inhibition	628:637	inhibition of cellulose synthesis	628:660	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	9	10	from	TA	1418:1419	arg1	cells					1452:1456	suspension-cultured cells	1432:1456	suspension-cultured cells	1432:1456	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	11	11	theme	CONCLUSIONS	1689:1699	arg1	Addition					1701:1708	CONCLUSIONS Addition	1689:1708	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures	1689:1757	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures prevented the TA- and IXB-mediated induction of cell death.					
31752698	8	12	theme	wide	1166:1169	arg1	range					1171:1175	a wide range	1164:1175	a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation	1164:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	8	12	theme	wide	1166:1169	arg1	role					1216:1219	a role	1214:1219	a role in the control of cell wall composition and rigidity to facilitate cell elongation	1214:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	11	13	theme	cell	1734:1737	arg1	cultures					1750:1757	Arabidopsis cell suspension cultures	1722:1757	Arabidopsis cell suspension cultures	1722:1757	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures prevented the TA- and IXB-mediated induction of cell death.					
31752698	8	14	from	cells	1197:1201	arg1	range					1171:1175	a wide range	1164:1175	a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation	1164:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	8	14	from	cells	1197:1201	arg1	role					1216:1219	a role	1214:1219	a role in the control of cell wall composition and rigidity to facilitate cell elongation	1214:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	9	15	theme	synthesis	1405:1413	arg1	inhibition					1381:1390	inhibition	1381:1390	inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells	1381:1456	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	12	16	theme	polar	1870:1874	arg1	transport					1882:1890	polar auxin transport	1870:1890	polar auxin transport	1870:1890	Cell survival was also stimulated by inhibition of polar auxin transport during CBI-treatment.					
31752698	4	17	theme	cell	692:695	arg1	cultures					708:715	Arabidopsis thaliana cell suspension cultures	671:715	Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB)	671:797	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	15	18	dep	limit	2311:2315	arg1	restoring					2339:2347	restoring	2339:2347	restoring its own transport	2339:2365	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	15	18	dep	limit	2311:2315	arg1	stabilizing					2377:2387	stabilizing	2377:2387	stabilizing the plasma membrane - cell wall - cytoskeleton continuum	2377:2444	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	8	19	contain	has	1160:1162	arg1	Auxin					1154:1158	Auxin	1154:1158	Auxin	1154:1158	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	8	19	contain	has	1160:1162	arg2	role					1216:1219	a role	1214:1219	a role in the control of cell wall composition and rigidity to facilitate cell elongation	1214:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	8	19	contain	has	1160:1162	arg2	range					1171:1175	a wide range	1164:1175	a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation	1164:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	8	20	from	role	1216:1219	arg1	control					1228:1234	the control	1224:1234	the control of cell wall composition and rigidity to facilitate cell elongation	1224:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	8	21	theme	wall	1244:1247	arg1	control					1228:1234	the control	1224:1234	the control of cell wall composition and rigidity to facilitate cell elongation	1224:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	0	22	theme	programmed	66:75	arg1	death					82:86	programmed cell death	66:86	programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben	66:160	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	13	23	theme	cells	2005:2009	arg1	properties					1979:1988	cell wall mechanical properties	1958:1988	cell wall mechanical properties of Arabidopsis cells	1958:2009	Inhibition of cellulose synthesis perturbed cell wall mechanical properties of Arabidopsis cells.					
31752698	0	24	theme	Arabidopsis	15:25	arg1	cultures					52:59	Arabidopsis thaliana cell suspension cultures	15:59	Arabidopsis thaliana cell suspension cultures	15:59	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	9	25	theme	suspension-cultured	1432:1450	arg1	cells					1452:1456	suspension-cultured cells	1432:1456	suspension-cultured cells	1432:1456	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	14	26	theme	wall	2066:2069	arg1	stiffness					2071:2079	cell wall stiffness	2061:2079	cell wall stiffness	2061:2079	Auxin treatment alone or with CBI also decreased cell wall stiffness, showing that the mechanical properties of the cell wall perturbed by CBIs were not restored by auxin.					
31752698	4	27	theme	Arabidopsis	671:681	arg1	cultures					708:715	Arabidopsis thaliana cell suspension cultures	671:715	Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB)	671:797	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	0	28	dep	inhibitors	126:135	arg1	A					147:147	thaxtomin A	137:147	thaxtomin A	137:147	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	0	28	dep	inhibitors	126:135	arg1	inhibitors					126:135	the cellulose biosynthesis inhibitors	99:135	the cellulose biosynthesis inhibitors thaxtomin A and isoxaben	99:160	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	0	28	dep	inhibitors	126:135	arg1	isoxaben					153:160	isoxaben	153:160	isoxaben	153:160	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	13	29	theme	mechanical	1968:1977	arg1	properties					1979:1988	cell wall mechanical properties	1958:1988	cell wall mechanical properties of Arabidopsis cells	1958:2009	Inhibition of cellulose synthesis perturbed cell wall mechanical properties of Arabidopsis cells.					
31752698	15	30	theme	cell	2411:2414	arg1	wall					2416:2419	cell wall	2411:2419	the plasma membrane - cell wall - cytoskeleton continuum	2389:2444	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	9	31	theme	wall	1473:1476	arg1	stiffness					1478:1486	cell wall stiffness	1468:1486	cell wall stiffness	1468:1486	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	8	32	theme	cell	1288:1291	arg1	elongation					1293:1302	cell elongation	1288:1302	cell elongation	1288:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	0	33	theme	cellulose	103:111	arg1	A					147:147	thaxtomin A	137:147	thaxtomin A	137:147	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	0	33	theme	cellulose	103:111	arg1	inhibitors					126:135	the cellulose biosynthesis inhibitors	99:135	the cellulose biosynthesis inhibitors thaxtomin A and isoxaben	99:160	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	0	33	theme	cellulose	103:111	arg1	isoxaben					153:160	isoxaben	153:160	isoxaben	153:160	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	4	34	used	used	666:669	arg2	we					663:664	we	663:664	we	663:664	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	6	35	theme	inhibitors	1030:1039	arg1	addition					1005:1012	The addition	1001:1012	The addition of auxin efflux inhibitors	1001:1039	The addition of auxin efflux inhibitors also inhibited the CBI-mediated induction of PCD.					
31752698	2	36	theme	program	450:456	arg1	induction					425:433	the induction	421:433	the induction of an atypical program of cell death (PCD)	421:476	Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD).					
31752698	10	37	theme	cell	1558:1561	arg1	stiffness					1568:1576	the cell wall stiffness	1554:1576	the cell wall stiffness in cells pretreated with auxin prior to CBI treatment	1554:1630	However, the cell wall stiffness in cells pretreated with auxin prior to CBI treatment was equivalent to that of cells treated with auxin only.					
31752698	10	37	theme	cell	1558:1561	arg1	equivalent					1636:1645	equivalent	1636:1645	equivalent	1636:1645	However, the cell wall stiffness in cells pretreated with auxin prior to CBI treatment was equivalent to that of cells treated with auxin only.					
31752698	1	38	theme	potato	262:267	arg1	scabies					311:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	5	39	theme	auxin	906:910	arg1	2,4-D					944:948	2,4-D	944:948	2,4-D	944:948	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	39	theme	auxin	906:910	arg1	acid					938:941	the synthetic auxin 2,4-dichlorophenoxyacetic acid	892:941	the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D)	892:949	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	6	40	theme	auxin	1017:1021	arg1	inhibitors					1030:1039	auxin efflux inhibitors	1017:1039	auxin efflux inhibitors	1017:1039	The addition of auxin efflux inhibitors also inhibited the CBI-mediated induction of PCD.					
31752698	7	41	theme	intracellular	1117:1129	arg1	accumulation					1131:1142	intracellular accumulation	1117:1142	intracellular accumulation of auxin	1117:1151	This effect may be due to intracellular accumulation of auxin.					
31752698	1	42	theme	scab-causing	276:287	arg1	scabies					311:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	9	43	theme	different	1508:1516	arg1	level					1493:1497	a level	1491:1497	a level slightly different than that caused by auxin	1491:1542	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	0	44	theme	cell	36:39	arg1	cultures					52:59	Arabidopsis thaliana cell suspension cultures	15:59	Arabidopsis thaliana cell suspension cultures	15:59	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	1	45	theme	Streptomyces	298:309	arg1	scabies					311:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	2	46	theme	cell	461:464	arg1	PCD					473:475	PCD	473:475	PCD	473:475	Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD).					
31752698	2	46	theme	cell	461:464	arg1	death					466:470	cell death	461:470	cell death (PCD)	461:476	Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD).					
31752698	10	47	theme	CBI	1618:1620	arg1	treatment					1622:1630	CBI treatment	1618:1630	CBI treatment	1618:1630	However, the cell wall stiffness in cells pretreated with auxin prior to CBI treatment was equivalent to that of cells treated with auxin only.					
31752698	10	48	from	stiffness	1568:1576	arg1	cells					1581:1585	cells	1581:1585	cells pretreated with auxin prior to CBI treatment	1581:1630	However, the cell wall stiffness in cells pretreated with auxin prior to CBI treatment was equivalent to that of cells treated with auxin only.					
31752698	13	49	theme	wall	1963:1966	arg1	properties					1979:1988	cell wall mechanical properties	1958:1988	cell wall mechanical properties of Arabidopsis cells	1958:2009	Inhibition of cellulose synthesis perturbed cell wall mechanical properties of Arabidopsis cells.					
31752698	2	50	theme	cellulose	334:342	arg1	synthesis					344:352	cellulose synthesis	334:352	cellulose synthesis	334:352	Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD).					
31752698	9	51	theme	Force	1318:1322	arg1	AFM					1336:1338	AFM	1336:1338	AFM	1336:1338	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	9	51	theme	Force	1318:1322	arg1	Microscopy					1324:1333	Atomic Force Microscopy	1311:1333	Atomic Force Microscopy (AFM)-based force spectroscopy	1311:1364	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	5	52	theme	natural	959:965	arg1	IAA					995:997	IAA	995:997	IAA	995:997	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	52	theme	natural	959:965	arg1	acid					989:992	the natural auxin indole-3-acetic acid	955:992	the natural auxin indole-3-acetic acid (IAA)	955:998	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	53	dep	RESULTS	800:806	arg1	induction					812:820	The induction	808:820	The induction of cell death by TA	808:840	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	53	dep	RESULTS	800:806	arg1	RESULTS					800:806	RESULTS	800:806	RESULTS The induction of cell death by TA and IXB	800:848	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	53	dep	RESULTS	800:806	arg1	IXB					846:848	IXB	846:848	IXB	846:848	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	2	54	theme	wall	377:380	arg1	organization					382:393	cell wall organization	372:393	cell wall organization	372:393	Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD).					
31752698	15	55	theme	cell	2222:2225	arg1	stiffness					2232:2240	the cell wall stiffness	2218:2240	the cell wall stiffness	2218:2240	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	5	56	theme	indole-3-acetic	973:987	arg1	IAA					995:997	IAA	995:997	IAA	995:997	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	56	theme	indole-3-acetic	973:987	arg1	acid					989:992	the natural auxin indole-3-acetic acid	955:992	the natural auxin indole-3-acetic acid (IAA)	955:998	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	6	57	theme	CBI-mediated	1060:1071	arg1	induction					1073:1081	the CBI-mediated induction	1056:1081	the CBI-mediated induction of PCD	1056:1088	The addition of auxin efflux inhibitors also inhibited the CBI-mediated induction of PCD.					
31752698	13	58	theme	cellulose	1928:1936	arg1	synthesis					1938:1946	cellulose synthesis	1928:1946	cellulose synthesis	1928:1946	Inhibition of cellulose synthesis perturbed cell wall mechanical properties of Arabidopsis cells.					
31752698	9	59	theme	force	1347:1351	arg1	spectroscopy					1353:1364	Atomic Force Microscopy (AFM)-based force spectroscopy	1311:1364	Atomic Force Microscopy (AFM)-based force spectroscopy	1311:1364	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	14	60	theme	cell	2128:2131	arg1	wall					2133:2136	the cell wall	2124:2136	the cell wall perturbed by CBIs	2124:2154	Auxin treatment alone or with CBI also decreased cell wall stiffness, showing that the mechanical properties of the cell wall perturbed by CBIs were not restored by auxin.					
31752698	3	61	theme	scabies	513:519	arg1	entry					521:525	S. scabies entry	510:525	S. scabies entry into plant tissues	510:544	These processes may facilitate S. scabies entry into plant tissues.					
31752698	5	62	theme	cell	825:828	arg1	death					830:834	cell death	825:834	cell death	825:834	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	8	63	theme	effects	1180:1186	arg1	range					1171:1175	a wide range	1164:1175	a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation	1164:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	8	63	theme	effects	1180:1186	arg1	role					1216:1219	a role	1214:1219	a role in the control of cell wall composition and rigidity to facilitate cell elongation	1214:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	12	64	theme	auxin	1876:1880	arg1	transport					1882:1890	polar auxin transport	1870:1890	polar auxin transport	1870:1890	Cell survival was also stimulated by inhibition of polar auxin transport during CBI-treatment.					
31752698	8	65	theme	plant	1191:1195	arg1	cells					1197:1201	plant cells	1191:1201	plant cells	1191:1201	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	14	66	theme	mechanical	2099:2108	arg1	properties					2110:2119	the mechanical properties	2095:2119	the mechanical properties of the cell wall perturbed by CBIs	2095:2154	Auxin treatment alone or with CBI also decreased cell wall stiffness, showing that the mechanical properties of the cell wall perturbed by CBIs were not restored by auxin.					
31752698	1	67	theme	biosynthesis	214:225	arg1	A					184:184	BACKGROUND Thaxtomin A	163:184	BACKGROUND Thaxtomin A (TA)	163:189	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	67	theme	biosynthesis	214:225	arg1	CBI					238:240	CBI	238:240	CBI	238:240	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	67	theme	biosynthesis	214:225	arg1	inhibitor					227:235	a natural cellulose biosynthesis inhibitor	194:235	a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies	194:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	0	68	theme	thaxtomin	137:145	arg1	A					147:147	thaxtomin A	137:147	thaxtomin A	137:147	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	0	68	theme	thaxtomin	137:145	arg1	inhibitors					126:135	the cellulose biosynthesis inhibitors	99:135	the cellulose biosynthesis inhibitors thaxtomin A and isoxaben	99:160	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	15	69	from	effects	2207:2213	arg1	stiffness					2232:2240	the cell wall stiffness	2218:2240	the cell wall stiffness	2218:2240	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	1	70	theme	Thaxtomin	174:182	arg1	TA					187:188	TA	187:188	TA	187:188	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	70	theme	Thaxtomin	174:182	arg1	A					184:184	BACKGROUND Thaxtomin A	163:184	BACKGROUND Thaxtomin A (TA)	163:189	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	70	theme	Thaxtomin	174:182	arg1	inhibitor					227:235	a natural cellulose biosynthesis inhibitor	194:235	a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies	194:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	4	71	theme	death	607:611	arg1	induction					589:597	the induction	585:597	the induction of cell death in response to inhibition of cellulose synthesis	585:660	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	11	72	theme	TA-	1773:1775	arg1	induction					1794:1802	the TA- and IXB-mediated induction	1769:1802	the TA- and IXB-mediated induction of cell death	1769:1816	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures prevented the TA- and IXB-mediated induction of cell death.					
31752698	4	73	theme	cellulose	642:650	arg1	synthesis					652:660	cellulose synthesis	642:660	cellulose synthesis	642:660	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	9	74	theme	cellulose	1395:1403	arg1	synthesis					1405:1413	cellulose synthesis	1395:1413	cellulose synthesis	1395:1413	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	11	75	theme	suspension	1739:1748	arg1	cultures					1750:1757	Arabidopsis cell suspension cultures	1722:1757	Arabidopsis cell suspension cultures	1722:1757	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures prevented the TA- and IXB-mediated induction of cell death.					
31752698	11	76	theme	auxin	1713:1717	arg1	Addition					1701:1708	CONCLUSIONS Addition	1689:1708	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures	1689:1757	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures prevented the TA- and IXB-mediated induction of cell death.					
31752698	11	77	theme	Arabidopsis	1722:1732	arg1	cultures					1750:1757	Arabidopsis cell suspension cultures	1722:1757	Arabidopsis cell suspension cultures	1722:1757	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures prevented the TA- and IXB-mediated induction of cell death.					
31752698	8	78	from	effects	1180:1186	arg1	cells					1197:1201	plant cells	1191:1201	plant cells	1191:1201	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	9	79	theme	cell	1468:1471	arg1	stiffness					1478:1486	cell wall stiffness	1468:1486	cell wall stiffness	1468:1486	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	4	80	theme	suspension	697:706	arg1	cultures					708:715	Arabidopsis thaliana cell suspension cultures	671:715	Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB)	671:797	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	0	81	theme	cell	77:80	arg1	death					82:86	programmed cell death	66:86	programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben	66:160	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	15	82	theme	own	2353:2355	arg1	transport					2357:2365	its own transport	2349:2365	its own transport	2349:2365	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	8	83	theme	cell	1239:1242	arg1	wall					1244:1247	cell wall composition and rigidity	1239:1272	wall	1244:1247	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	4	84	from	induction	589:597	arg1	response					616:623	response	616:623	response to inhibition of cellulose synthesis	616:660	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	15	85	theme	CBIs	2331:2334	arg1	impact					2321:2326	the impact	2317:2326	the impact of CBIs	2317:2334	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	1	86	theme	BACKGROUND	163:172	arg1	TA					187:188	TA	187:188	TA	187:188	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	86	theme	BACKGROUND	163:172	arg1	A					184:184	BACKGROUND Thaxtomin A	163:184	BACKGROUND Thaxtomin A (TA)	163:189	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	86	theme	BACKGROUND	163:172	arg1	inhibitor					227:235	a natural cellulose biosynthesis inhibitor	194:235	a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies	194:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	14	87	theme	cell	2061:2064	arg1	stiffness					2071:2079	cell wall stiffness	2061:2079	cell wall stiffness	2061:2079	Auxin treatment alone or with CBI also decreased cell wall stiffness, showing that the mechanical properties of the cell wall perturbed by CBIs were not restored by auxin.					
31752698	15	88	dep	membrane	2400:2407	arg1	wall					2416:2419	cell wall	2411:2419	the plasma membrane - cell wall - cytoskeleton continuum	2389:2444	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	15	88	dep	membrane	2400:2407	arg1	continuum					2436:2444	continuum	2436:2444	the plasma membrane - cell wall - cytoskeleton continuum	2389:2444	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	15	88	dep	membrane	2400:2407	arg1	cytoskeleton					2423:2434	cytoskeleton	2423:2434	the plasma membrane - cell wall - cytoskeleton continuum	2389:2444	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	0	89	dep	Arabidopsis	15:25	arg1	thaliana					27:34	thaliana	27:34	thaliana	27:34	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	4	90	dep	Arabidopsis	671:681	arg1	thaliana					683:690	thaliana	683:690	thaliana	683:690	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	8	91	theme	rigidity	1265:1272	arg1	control					1228:1234	the control	1224:1234	the control of cell wall composition and rigidity to facilitate cell elongation	1224:1302	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	4	92	theme	different	747:755	arg1	CBIs					757:760	two structurally different CBIs	730:760	two structurally different CBIs	730:760	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	4	92	theme	different	747:755	arg1	isoxaben					784:791	the herbicide isoxaben	770:791	the herbicide isoxaben (IXB)	770:797	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	4	92	theme	different	747:755	arg1	TA					763:764	TA	763:764	TA	763:764	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	0	93	theme	biosynthesis	113:124	arg1	A					147:147	thaxtomin A	137:147	thaxtomin A	137:147	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	0	93	theme	biosynthesis	113:124	arg1	inhibitors					126:135	the cellulose biosynthesis inhibitors	99:135	the cellulose biosynthesis inhibitors thaxtomin A and isoxaben	99:160	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	0	93	theme	biosynthesis	113:124	arg1	isoxaben					153:160	isoxaben	153:160	isoxaben	153:160	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	7	94	theme	auxin	1147:1151	arg1	accumulation					1131:1142	intracellular accumulation	1117:1142	intracellular accumulation of auxin	1117:1151	This effect may be due to intracellular accumulation of auxin.					
31752698	13	95	theme	Arabidopsis	1993:2003	arg1	cells					2005:2009	Arabidopsis cells	1993:2009	Arabidopsis cells	1993:2009	Inhibition of cellulose synthesis perturbed cell wall mechanical properties of Arabidopsis cells.					
31752698	12	96	theme	Cell	1819:1822	arg1	survival					1824:1831	Cell survival	1819:1831	Cell survival	1819:1831	Cell survival was also stimulated by inhibition of polar auxin transport during CBI-treatment.					
31752698	5	97	theme	synthetic	896:904	arg1	2,4-D					944:948	2,4-D	944:948	2,4-D	944:948	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	97	theme	synthetic	896:904	arg1	acid					938:941	the synthetic auxin 2,4-dichlorophenoxyacetic acid	892:941	the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D)	892:949	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	6	98	theme	efflux	1023:1028	arg1	inhibitors					1030:1039	auxin efflux inhibitors	1017:1039	auxin efflux inhibitors	1017:1039	The addition of auxin efflux inhibitors also inhibited the CBI-mediated induction of PCD.					
31752698	2	99	theme	atypical	441:448	arg1	program					450:456	an atypical program	438:456	an atypical program of cell death (PCD)	438:476	Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD).					
31752698	5	100	with	pretreatment	874:885	arg1	2,4-D					944:948	2,4-D	944:948	2,4-D	944:948	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	100	with	pretreatment	874:885	arg1	acid					938:941	the synthetic auxin 2,4-dichlorophenoxyacetic acid	892:941	the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D)	892:949	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	100	with	pretreatment	874:885	arg1	IAA					995:997	IAA	995:997	IAA	995:997	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	100	with	pretreatment	874:885	arg1	acid					989:992	the natural auxin indole-3-acetic acid	955:992	the natural auxin indole-3-acetic acid (IAA)	955:998	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	11	101	theme	death	1812:1816	arg1	induction					1794:1802	the TA- and IXB-mediated induction	1769:1802	the TA- and IXB-mediated induction of cell death	1769:1816	CONCLUSIONS Addition of auxin to Arabidopsis cell suspension cultures prevented the TA- and IXB-mediated induction of cell death.					
31752698	1	102	theme	common	269:274	arg1	scabies					311:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	15	103	theme	plasma	2393:2398	arg1	membrane					2400:2407	the plasma membrane	2389:2407	the plasma membrane - cell wall - cytoskeleton continuum	2389:2444	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31752698	5	104	theme	2,4-dichlorophenoxyacetic	912:936	arg1	2,4-D					944:948	2,4-D	944:948	2,4-D	944:948	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	104	theme	2,4-dichlorophenoxyacetic	912:936	arg1	acid					938:941	the synthetic auxin 2,4-dichlorophenoxyacetic acid	892:941	the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D)	892:949	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	14	105	theme	Auxin	2012:2016	arg1	treatment					2018:2026	Auxin treatment	2012:2026	Auxin treatment alone or with CBI	2012:2044	Auxin treatment alone or with CBI also decreased cell wall stiffness, showing that the mechanical properties of the cell wall perturbed by CBIs were not restored by auxin.					
31752698	0	106	theme	suspension	41:50	arg1	cultures					52:59	Arabidopsis thaliana cell suspension cultures	15:59	Arabidopsis thaliana cell suspension cultures	15:59	Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben.					
31752698	1	107	theme	pathogen	289:296	arg1	scabies					311:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	the potato common scab-causing pathogen Streptomyces scabies	258:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	108	theme	natural	196:202	arg1	A					184:184	BACKGROUND Thaxtomin A	163:184	BACKGROUND Thaxtomin A (TA)	163:189	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	108	theme	natural	196:202	arg1	CBI					238:240	CBI	238:240	CBI	238:240	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	108	theme	natural	196:202	arg1	inhibitor					227:235	a natural cellulose biosynthesis inhibitor	194:235	a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies	194:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	2	109	theme	death	466:470	arg1	program					450:456	an atypical program	438:456	an atypical program of cell death (PCD)	438:476	Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD).					
31752698	13	110	theme	synthesis	1938:1946	arg1	Inhibition					1914:1923	Inhibition	1914:1923	Inhibition of cellulose synthesis	1914:1946	Inhibition of cellulose synthesis perturbed cell wall mechanical properties of Arabidopsis cells.					
31752698	8	111	dep	wall	1244:1247	arg1	composition					1249:1259	composition	1249:1259	composition	1249:1259	Auxin has a wide range of effects in plant cells, including a role in the control of cell wall composition and rigidity to facilitate cell elongation.					
31752698	13	112	theme	cell	1958:1961	arg1	properties					1979:1988	cell wall mechanical properties	1958:1988	cell wall mechanical properties of Arabidopsis cells	1958:2009	Inhibition of cellulose synthesis perturbed cell wall mechanical properties of Arabidopsis cells.					
31752698	2	113	theme	synthesis	344:352	arg1	Inhibition					320:329	Inhibition	320:329	Inhibition of cellulose synthesis by TA	320:358	Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD).					
31752698	9	114	theme	Atomic	1311:1316	arg1	AFM					1336:1338	AFM	1336:1338	AFM	1336:1338	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	9	114	theme	Atomic	1311:1316	arg1	Microscopy					1324:1333	Atomic Force Microscopy	1311:1333	Atomic Force Microscopy (AFM)-based force spectroscopy	1311:1364	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	6	115	theme	PCD	1086:1088	arg1	induction					1073:1081	the CBI-mediated induction	1056:1081	the CBI-mediated induction of PCD	1056:1088	The addition of auxin efflux inhibitors also inhibited the CBI-mediated induction of PCD.					
31752698	2	116	theme	cell	372:375	arg1	organization					382:393	cell wall organization	372:393	cell wall organization	372:393	Inhibition of cellulose synthesis by TA compromises cell wall organization and integrity, leading to the induction of an atypical program of cell death (PCD).					
31752698	9	117	theme	Microscopy	1324:1333	arg1	spectroscopy					1353:1364	Atomic Force Microscopy (AFM)-based force spectroscopy	1311:1364	Atomic Force Microscopy (AFM)-based force spectroscopy	1311:1364	Using Atomic Force Microscopy (AFM)-based force spectroscopy, we found that inhibition of cellulose synthesis by TA and IXB in suspension-cultured cells decreased cell wall stiffness to a level slightly different than that caused by auxin.					
31752698	1	118	theme	cellulose	204:212	arg1	A					184:184	BACKGROUND Thaxtomin A	163:184	BACKGROUND Thaxtomin A (TA)	163:189	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	118	theme	cellulose	204:212	arg1	CBI					238:240	CBI	238:240	CBI	238:240	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	1	118	theme	cellulose	204:212	arg1	inhibitor					227:235	a natural cellulose biosynthesis inhibitor	194:235	a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies	194:317	BACKGROUND Thaxtomin A (TA) is a natural cellulose biosynthesis inhibitor (CBI) synthesized by the potato common scab-causing pathogen Streptomyces scabies.					
31752698	5	119	theme	auxin	967:971	arg1	IAA					995:997	IAA	995:997	IAA	995:997	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	5	119	theme	auxin	967:971	arg1	acid					989:992	the natural auxin indole-3-acetic acid	955:992	the natural auxin indole-3-acetic acid (IAA)	955:998	RESULTS The induction of cell death by TA and IXB was abrogated following pretreatment with the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the natural auxin indole-3-acetic acid (IAA).					
31752698	10	120	theme	wall	1563:1566	arg1	stiffness					1568:1576	the cell wall stiffness	1554:1576	the cell wall stiffness in cells pretreated with auxin prior to CBI treatment	1554:1630	However, the cell wall stiffness in cells pretreated with auxin prior to CBI treatment was equivalent to that of cells treated with auxin only.					
31752698	10	120	theme	wall	1563:1566	arg1	equivalent					1636:1645	equivalent	1636:1645	equivalent	1636:1645	However, the cell wall stiffness in cells pretreated with auxin prior to CBI treatment was equivalent to that of cells treated with auxin only.					
31752698	4	121	theme	cell	602:605	arg1	death					607:611	cell death	602:611	cell death	602:611	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	4	122	theme	herbicide	774:782	arg1	IXB					794:796	IXB	794:796	IXB	794:796	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	4	122	theme	herbicide	774:782	arg1	isoxaben					784:791	the herbicide isoxaben	770:791	the herbicide isoxaben (IXB)	770:797	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	4	122	theme	herbicide	774:782	arg1	CBIs					757:760	two structurally different CBIs	730:760	two structurally different CBIs	730:760	To study the mechanisms that regulate the induction of cell death in response to inhibition of cellulose synthesis, we used Arabidopsis thaliana cell suspension cultures treated with two structurally different CBIs, TA and the herbicide isoxaben (IXB).					
31752698	3	123	theme	S.	510:511	arg1	entry					521:525	S. scabies entry	510:525	S. scabies entry into plant tissues	510:544	These processes may facilitate S. scabies entry into plant tissues.					
31752698	15	124	theme	wall	2227:2230	arg1	stiffness					2232:2240	the cell wall stiffness	2218:2240	the cell wall stiffness	2218:2240	However, since auxin's effects on the cell wall stiffness apparently overrode those induced by CBIs, we suggest that auxin may limit the impact of CBIs by restoring its own transport and/or by stabilizing the plasma membrane - cell wall - cytoskeleton continuum.					
31676555	2	0	theme	free	321:324	arg1	O-glycans					326:334	free O-glycans	321:334	free O-glycans	321:334	Hirayama et al. now reveal, by using only mannose as an energy source, the generation of free O-glycans in Saccharomyces cerevisiae, in the lumen of a secretory compartment, possibly the vacuole.					
31676555	3	1	theme	possible	469:476	arg1	pathway					500:506	a possible regulated degradation pathway	467:506	a possible regulated degradation pathway for O-mannosylated glycoproteins	467:539	These findings uncover the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins.					
31676555	1	2	theme	free	43:46	arg1	N-glycans					48:56	free N-glycans	43:56	free N-glycans	43:56	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	3	3	theme	O-mannosylated	512:525	arg1	glycoproteins					527:539	O-mannosylated glycoproteins	512:539	O-mannosylated glycoproteins	512:539	These findings uncover the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins.					
31676555	2	4	theme	energy	288:293	arg1	source					295:300	an energy source	285:300	an energy source	285:300	Hirayama et al. now reveal, by using only mannose as an energy source, the generation of free O-glycans in Saccharomyces cerevisiae, in the lumen of a secretory compartment, possibly the vacuole.					
31676555	2	4	theme	energy	288:293	arg1	mannose					274:280	only mannose	269:280	only mannose	269:280	Hirayama et al. now reveal, by using only mannose as an energy source, the generation of free O-glycans in Saccharomyces cerevisiae, in the lumen of a secretory compartment, possibly the vacuole.					
31676555	1	5	link	O-linked	183:190	arg1	carbohydrates					205:217	O-linked glycoprotein carbohydrates	183:217	O-linked glycoprotein carbohydrates	183:217	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	2	6	from	generation	307:316	arg1	cerevisiae					353:362	Saccharomyces cerevisiae	339:362	Saccharomyces cerevisiae	339:362	Hirayama et al. now reveal, by using only mannose as an energy source, the generation of free O-glycans in Saccharomyces cerevisiae, in the lumen of a secretory compartment, possibly the vacuole.					
31676555	1	7	theme	N-glycans	48:56	arg1	oligosaccharides					75:90	unconjugated oligosaccharides	62:90	unconjugated oligosaccharides derived from N-linked glycoproteins	62:126	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	7	theme	N-glycans	48:56	arg1	generation					29:38	The generation	25:38	The generation of free N-glycans	25:56	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	7	theme	N-glycans	48:56	arg1	understood					137:146	understood	137:146	well understood	132:146	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	3	8	theme	degradation	488:498	arg1	pathway					500:506	a possible regulated degradation pathway	467:506	a possible regulated degradation pathway for O-mannosylated glycoproteins	467:539	These findings uncover the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins.					
31676555	3	9	theme	pathway	500:506	arg1	presence					455:462	the presence	451:462	the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins	451:539	These findings uncover the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins.					
31676555	3	10	theme	regulated	478:486	arg1	pathway					500:506	a possible regulated degradation pathway	467:506	a possible regulated degradation pathway for O-mannosylated glycoproteins	467:539	These findings uncover the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins.					
31676555	1	11	theme	unconjugated	62:73	arg1	oligosaccharides					75:90	unconjugated oligosaccharides	62:90	unconjugated oligosaccharides derived from N-linked glycoproteins	62:126	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	12	theme	similar	163:169	arg1	fate					171:174	a similar fate	161:174	a similar fate awaits O-linked glycoprotein carbohydrates	161:217	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	12	theme	similar	163:169	arg1	unknown					223:229	unknown	223:229	unknown	223:229	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	13	gly	glycoprotein	192:203	arg1	glycoprotein					192:203	O-linked glycoprotein carbohydrates	183:217	O-linked glycoprotein carbohydrates	183:217	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	2	14	theme	compartment	393:403	arg1	lumen					372:376	the lumen	368:376	the lumen of a secretory compartment	368:403	Hirayama et al. now reveal, by using only mannose as an energy source, the generation of free O-glycans in Saccharomyces cerevisiae, in the lumen of a secretory compartment, possibly the vacuole.					
31676555	1	15	gly	glycoproteins	114:126	arg1	glycoproteins					114:126	N-linked glycoproteins	105:126	N-linked glycoproteins	105:126	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	2	16	theme	secretory	383:391	arg1	compartment					393:403	a secretory compartment	381:403	a secretory compartment	381:403	Hirayama et al. now reveal, by using only mannose as an energy source, the generation of free O-glycans in Saccharomyces cerevisiae, in the lumen of a secretory compartment, possibly the vacuole.					
31676555	1	17	theme	O-linked	183:190	arg1	carbohydrates					205:217	O-linked glycoprotein carbohydrates	183:217	O-linked glycoprotein carbohydrates	183:217	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	18	theme	glycoprotein	192:203	arg1	carbohydrates					205:217	O-linked glycoprotein carbohydrates	183:217	O-linked glycoprotein carbohydrates	183:217	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	19	dep	fate	171:174	arg1	awaits					176:181	awaits	176:181	awaits O-linked glycoprotein carbohydrates	176:217	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	20	theme	N-linked	105:112	arg1	glycoproteins					114:126	N-linked glycoproteins	105:126	N-linked glycoproteins	105:126	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	21	attach	derived	92:98	arg2	oligosaccharides					75:90	unconjugated oligosaccharides	62:90	unconjugated oligosaccharides derived from N-linked glycoproteins	62:126	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	21	attach	derived	92:98	arg1	glycoproteins					114:126	N-linked glycoproteins	105:126	N-linked glycoproteins	105:126	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	1	22	link	N-linked	105:112	arg1	glycoproteins					114:126	N-linked glycoproteins	105:126	N-linked glycoproteins	105:126	The generation of free N-glycans, or unconjugated oligosaccharides derived from N-linked glycoproteins, is well understood, but whether a similar fate awaits O-linked glycoprotein carbohydrates was unknown.					
31676555	3	23	gly	glycoproteins	527:539	arg1	glycoproteins					527:539	O-mannosylated glycoproteins	512:539	O-mannosylated glycoproteins	512:539	These findings uncover the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins.					
31676555	3	24	gly	O-mannosylated	512:525	arg1	glycoproteins					527:539	O-mannosylated glycoproteins	512:539	O-mannosylated glycoproteins	512:539	These findings uncover the presence of a possible regulated degradation pathway for O-mannosylated glycoproteins.					
31676555	2	25	theme	O-glycans	326:334	arg1	generation					307:316	the generation	303:316	the generation of free O-glycans in Saccharomyces cerevisiae	303:362	Hirayama et al. now reveal, by using only mannose as an energy source, the generation of free O-glycans in Saccharomyces cerevisiae, in the lumen of a secretory compartment, possibly the vacuole.					
30563124	6	0	theme	phosphate	1091:1099	arg1	hydrolysis					1052:1061	the hydrolysis	1048:1061	the hydrolysis of the substrate α-naphthyl phosphate, which produced an electrochemical signal	1048:1141	Lastly, alkaline phosphatase catalyzed the hydrolysis of the substrate α-naphthyl phosphate, which produced an electrochemical signal.					
30563124	6	1	theme	electrochemical	1120:1134	arg1	signal					1136:1141	an electrochemical signal	1117:1141	an electrochemical signal	1117:1141	Lastly, alkaline phosphatase catalyzed the hydrolysis of the substrate α-naphthyl phosphate, which produced an electrochemical signal.					
30563124	3	2	theme	carbon	528:533	arg1	composite					572:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established.					
30563124	4	3	theme	electrochemical	620:634	arg1	immunosensor					636:647	the electrochemical immunosensor	616:647	the electrochemical immunosensor	616:647	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	1	4	dep	animal	274:279	arg1	feeds					281:285	feeds	281:285	feeds	281:285	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	3	5	theme	nanotubes/chitosan	535:552	arg1	composite					572:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established.					
30563124	1	6	theme	human	261:265	arg1	foods					267:271	human foods	261:271	human foods	261:271	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	0	7	theme	Toxin	134:138	arg1	Determination					113:125	Sensitive Determination	103:125	Sensitive Determination of T-2 Toxin in Feed and Swine Meat	103:161	Construction of Electrochemical Immunosensor Based on Gold-Nanoparticles/Carbon Nanotubes/Chitosan for Sensitive Determination of T-2 Toxin in Feed and Swine Meat.					
30563124	5	8	theme	electrode	952:960	arg1	surface					962:968	the electrode surface	948:968	the electrode surface	948:968	Afterwards, the alkaline phosphatase labeled anti-mouse secondary antibody was bound to the electrode surface by reacting with the primary antibody.					
30563124	8	9	dep	100	1324:1326	arg1	to					1321:1322	to	1321:1322	to	1321:1322	Under optimal conditions, this method could quantitatively detect T-2 from 0.01 to 100 μg·L-1 with a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%.					
30563124	0	10	theme	T-2	130:132	arg1	Toxin					134:138	T-2 Toxin	130:138	T-2 Toxin	130:138	Construction of Electrochemical Immunosensor Based on Gold-Nanoparticles/Carbon Nanotubes/Chitosan for Sensitive Determination of T-2 Toxin in Feed and Swine Meat.					
30563124	4	11	theme	serum	747:751	arg1	albumin					753:759	T-2-bovine serum albumin	736:759	T-2-bovine serum albumin	736:759	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	4	12	theme	immunosensor	636:647	arg1	mechanism					603:611	The mechanism	599:611	The mechanism of the electrochemical immunosensor	599:647	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	4	12	theme	immunosensor	636:647	arg1	binding					677:683	an indirect competitive binding	653:683	an indirect competitive binding to a given amount of anti-T-2	653:713	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	4	13	theme	T-2-bovine	736:745	arg1	albumin					753:759	T-2-bovine serum albumin	736:759	T-2-bovine serum albumin	736:759	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	9	14	theme	liquid	1561:1566	arg1	LC-MS/MS					1609:1616	LC-MS/MS	1609:1616	LC-MS/MS	1609:1616	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	9	14	theme	liquid	1561:1566	arg1	spectrometry					1595:1606	liquid chromatography-tandem mass spectrometry	1561:1606	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1561:1617	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	5	15	theme	labeled	897:903	arg1	antibody					926:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	Afterwards, the alkaline phosphatase labeled anti-mouse secondary antibody was bound to the electrode surface by reacting with the primary antibody.					
30563124	4	16	theme	free	723:726	arg1	T-2					728:730	free T-2	723:730	free T-2	723:730	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	2	17	theme	toxin	384:388	arg1	detection					390:398	accurate T-2 toxin detection	371:398	accurate T-2 toxin detection	371:398	Thus, a facile and sensitive method is essential for accurate T-2 toxin detection.					
30563124	6	18	theme	substrate	1070:1078	arg1	phosphate					1091:1099	the substrate α-naphthyl phosphate	1066:1099	the substrate α-naphthyl phosphate	1066:1099	Lastly, alkaline phosphatase catalyzed the hydrolysis of the substrate α-naphthyl phosphate, which produced an electrochemical signal.					
30563124	9	19	theme	chromatography-tandem	1568:1588	arg1	LC-MS/MS					1609:1616	LC-MS/MS	1609:1616	LC-MS/MS	1609:1616	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	9	19	theme	chromatography-tandem	1568:1588	arg1	spectrometry					1595:1606	liquid chromatography-tandem mass spectrometry	1561:1606	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1561:1617	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	2	20	theme	T-2	380:382	arg1	detection					390:398	accurate T-2 toxin detection	371:398	accurate T-2 toxin detection	371:398	Thus, a facile and sensitive method is essential for accurate T-2 toxin detection.					
30563124	6	21	theme	alkaline	1017:1024	arg1	phosphatase					1026:1036	alkaline phosphatase	1017:1036	alkaline phosphatase	1017:1036	Lastly, alkaline phosphatase catalyzed the hydrolysis of the substrate α-naphthyl phosphate, which produced an electrochemical signal.					
30563124	2	22	theme	accurate	371:378	arg1	detection					390:398	accurate T-2 toxin detection	371:398	accurate T-2 toxin detection	371:398	Thus, a facile and sensitive method is essential for accurate T-2 toxin detection.					
30563124	9	23	theme	mass	1590:1593	arg1	LC-MS/MS					1609:1616	LC-MS/MS	1609:1616	LC-MS/MS	1609:1616	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	9	23	theme	mass	1590:1593	arg1	spectrometry					1595:1606	liquid chromatography-tandem mass spectrometry	1561:1606	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1561:1617	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	8	24	theme	detection	1342:1350	arg1	limit					1352:1356	a detection limit	1340:1356	a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%	1340:1408	Under optimal conditions, this method could quantitatively detect T-2 from 0.01 to 100 μg·L-1 with a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%.					
30563124	0	25	theme	Immunosensor	32:43	arg1	Construction					0:11	Construction	0:11	Construction of Electrochemical Immunosensor	0:43	Construction of Electrochemical Immunosensor Based on Gold-Nanoparticles/Carbon Nanotubes/Chitosan for Sensitive Determination of T-2 Toxin in Feed and Swine Meat.					
30563124	5	26	theme	anti-mouse	905:914	arg1	antibody					926:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	Afterwards, the alkaline phosphatase labeled anti-mouse secondary antibody was bound to the electrode surface by reacting with the primary antibody.					
30563124	0	27	theme	Swine	152:156	arg1	Meat					158:161	Swine Meat	152:161	Swine Meat	152:161	Construction of Electrochemical Immunosensor Based on Gold-Nanoparticles/Carbon Nanotubes/Chitosan for Sensitive Determination of T-2 Toxin in Feed and Swine Meat.					
30563124	5	28	theme	primary	991:997	arg1	antibody					999:1006	the primary antibody	987:1006	the primary antibody	987:1006	Afterwards, the alkaline phosphatase labeled anti-mouse secondary antibody was bound to the electrode surface by reacting with the primary antibody.					
30563124	4	29	theme	functionalized	797:810	arg1	cSWNTs					812:817	covalently functionalized cSWNTs	786:817	covalently functionalized cSWNTs decorated on the glass carbon electrode	786:857	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	1	30	theme	major	190:194	arg1	mycotoxins					204:213	major concern mycotoxins	190:213	major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products	190:315	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	0	31	theme	Electrochemical	16:30	arg1	Immunosensor					32:43	Electrochemical Immunosensor	16:43	Electrochemical Immunosensor	16:43	Construction of Electrochemical Immunosensor Based on Gold-Nanoparticles/Carbon Nanotubes/Chitosan for Sensitive Determination of T-2 Toxin in Feed and Swine Meat.					
30563124	7	32	theme	conventional	1158:1169	arg1	methods					1171:1177	conventional methods	1158:1177	conventional methods	1158:1177	Compared with conventional methods, the established immunosensor was more sensitive and simpler.					
30563124	9	33	dep	feed	1479:1482	arg1	meat					1494:1497	meat	1494:1497	meat	1494:1497	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	2	34	theme	sensitive	337:345	arg1	method					347:352	a facile and sensitive method	324:352	a facile and sensitive method	324:352	Thus, a facile and sensitive method is essential for accurate T-2 toxin detection.					
30563124	2	34	theme	sensitive	337:345	arg1	essential					357:365	essential	357:365	essential	357:365	Thus, a facile and sensitive method is essential for accurate T-2 toxin detection.					
30563124	1	35	theme	concern	196:202	arg1	mycotoxins					204:213	major concern mycotoxins	190:213	major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products	190:315	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	3	36	theme	single-walled	514:526	arg1	composite					572:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established.					
30563124	0	37	from	Determination	113:125	arg1	Meat					158:161	Swine Meat	152:161	Swine Meat	152:161	Construction of Electrochemical Immunosensor Based on Gold-Nanoparticles/Carbon Nanotubes/Chitosan for Sensitive Determination of T-2 Toxin in Feed and Swine Meat.					
30563124	0	37	from	Determination	113:125	arg1	Feed					143:146	Feed	143:146	Feed	143:146	Construction of Electrochemical Immunosensor Based on Gold-Nanoparticles/Carbon Nanotubes/Chitosan for Sensitive Determination of T-2 Toxin in Feed and Swine Meat.					
30563124	8	38	theme	%	1408:1408	arg1	limit					1352:1356	a detection limit	1340:1356	a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%	1340:1408	Under optimal conditions, this method could quantitatively detect T-2 from 0.01 to 100 μg·L-1 with a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%.					
30563124	1	39	theme	mycotoxins	204:213	arg1	one					183:185	one	183:185	one	183:185	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	1	39	theme	mycotoxins	204:213	arg1	mycotoxins					204:213	major concern mycotoxins	190:213	major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products	190:315	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	4	40	theme	anti-T-2	706:713	arg1	anti-T-2					706:713	anti-T-2	706:713	anti-T-2	706:713	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	4	40	theme	anti-T-2	706:713	arg1	amount					696:701	a given amount	688:701	a given amount of anti-T-2	688:713	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	1	41	theme	agriculture	296:306	arg1	products					308:315	agriculture products	296:315	agriculture products	296:315	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	8	42	theme	0.13	1361:1364	arg1	μg·L-1					1366:1371	0.13 μg·L-1	1361:1371	0.13 μg·L-1	1361:1371	Under optimal conditions, this method could quantitatively detect T-2 from 0.01 to 100 μg·L-1 with a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%.					
30563124	6	43	theme	α-naphthyl	1080:1089	arg1	phosphate					1091:1099	the substrate α-naphthyl phosphate	1066:1099	the substrate α-naphthyl phosphate	1066:1099	Lastly, alkaline phosphatase catalyzed the hydrolysis of the substrate α-naphthyl phosphate, which produced an electrochemical signal.					
30563124	8	44	theme	favorable	1377:1385	arg1	%					1408:1408	favorable recovery 91.42⁻102.49%	1377:1408	favorable recovery 91.42⁻102.49%	1377:1408	Under optimal conditions, this method could quantitatively detect T-2 from 0.01 to 100 μg·L-1 with a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%.					
30563124	4	45	theme	given	690:694	arg1	anti-T-2					706:713	anti-T-2	706:713	anti-T-2	706:713	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	4	45	theme	given	690:694	arg1	amount					696:701	a given amount	688:701	a given amount of anti-T-2	688:713	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	9	46	theme	good	1513:1516	arg1	correlation					1518:1528	good correlation	1513:1528	good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1513:1617	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	9	47	with	correlation	1518:1528	arg1	results					1539:1545	the results	1535:1545	the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1535:1617	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	3	48	theme	group-functionalized	493:512	arg1	composite					572:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established.					
30563124	1	49	from	contaminant	246:256	arg1	products					308:315	agriculture products	296:315	agriculture products	296:315	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	1	49	from	contaminant	246:256	arg1	animal					274:279	animal	274:279	animal	274:279	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	1	49	from	contaminant	246:256	arg1	foods					267:271	human foods	261:271	human foods	261:271	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	4	50	dep	binding	677:683	arg1	conjugated					772:781	conjugated	772:781	was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode	768:857	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	7	51	theme	established	1184:1194	arg1	immunosensor					1196:1207	the established immunosensor	1180:1207	the established immunosensor	1180:1207	Compared with conventional methods, the established immunosensor was more sensitive and simpler.					
30563124	7	51	theme	established	1184:1194	arg1	sensitive					1218:1226	sensitive	1218:1226	sensitive	1218:1226	Compared with conventional methods, the established immunosensor was more sensitive and simpler.					
30563124	2	52	theme	facile	326:331	arg1	method					347:352	a facile and sensitive method	324:352	a facile and sensitive method	324:352	Thus, a facile and sensitive method is essential for accurate T-2 toxin detection.					
30563124	2	52	theme	facile	326:331	arg1	essential					357:365	essential	357:365	essential	357:365	Thus, a facile and sensitive method is essential for accurate T-2 toxin detection.					
30563124	9	53	from	T-2	1472:1474	arg1	feed					1479:1482	feed	1479:1482	feed	1479:1482	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	9	53	from	T-2	1472:1474	arg1	swine					1488:1492	swine	1488:1492	swine	1488:1492	Moreover, the immunosensor was successfully applied to assay T-2 in feed and swine meat, which showed good correlation with the results obtained from liquid chromatography-tandem mass spectrometry (LC-MS/MS).					
30563124	8	54	theme	optimal	1247:1253	arg1	conditions					1255:1264	optimal conditions	1247:1264	optimal conditions	1247:1264	Under optimal conditions, this method could quantitatively detect T-2 from 0.01 to 100 μg·L-1 with a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%.					
30563124	4	55	theme	carbon	842:847	arg1	electrode					849:857	the glass carbon electrode	832:857	the glass carbon electrode	832:857	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	3	56	theme	gold	463:466	arg1	composite					572:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established.					
30563124	4	57	theme	indirect	656:663	arg1	mechanism					603:611	The mechanism	599:611	The mechanism of the electrochemical immunosensor	599:647	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	4	57	theme	indirect	656:663	arg1	binding					677:683	an indirect competitive binding	653:683	an indirect competitive binding to a given amount of anti-T-2	653:713	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	4	58	theme	glass	836:840	arg1	electrode					849:857	the glass carbon electrode	832:857	the glass carbon electrode	832:857	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	3	59	theme	nanoparticles/carboxylic	468:491	arg1	composite					572:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established.					
30563124	8	60	theme	recovery	1387:1394	arg1	%					1408:1408	favorable recovery 91.42⁻102.49%	1377:1408	favorable recovery 91.42⁻102.49%	1377:1408	Under optimal conditions, this method could quantitatively detect T-2 from 0.01 to 100 μg·L-1 with a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%.					
30563124	0	61	theme	Sensitive	103:111	arg1	Determination					113:125	Sensitive Determination	103:125	Sensitive Determination of T-2 Toxin in Feed and Swine Meat	103:161	Construction of Electrochemical Immunosensor Based on Gold-Nanoparticles/Carbon Nanotubes/Chitosan for Sensitive Determination of T-2 Toxin in Feed and Swine Meat.					
30563124	5	62	theme	secondary	916:924	arg1	antibody					926:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	Afterwards, the alkaline phosphatase labeled anti-mouse secondary antibody was bound to the electrode surface by reacting with the primary antibody.					
30563124	3	63	theme	electrochemical	425:439	arg1	immunosensor					441:452	a specific electrochemical immunosensor	414:452	a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	414:580	In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established.					
30563124	5	64	theme	phosphatase	885:895	arg1	antibody					926:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	Afterwards, the alkaline phosphatase labeled anti-mouse secondary antibody was bound to the electrode surface by reacting with the primary antibody.					
30563124	1	65	theme	T-2	164:166	arg1	toxin					168:172	T-2 toxin	164:172	T-2 toxin (T-2)	164:178	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	1	65	theme	T-2	164:166	arg1	T-2					175:177	T-2	175:177	T-2	175:177	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	3	66	theme	specific	416:423	arg1	immunosensor					441:452	a specific electrochemical immunosensor	414:452	a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	414:580	In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established.					
30563124	1	67	theme	unavoidable	234:244	arg1	contaminant					246:256	an unavoidable contaminant	231:256	an unavoidable contaminant in human foods, animal feeds and also agriculture products	231:315	T-2 toxin (T-2) is one of major concern mycotoxins acknowledged as an unavoidable contaminant in human foods, animal feeds and also agriculture products.					
30563124	5	68	theme	alkaline	876:883	arg1	antibody					926:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	the alkaline phosphatase labeled anti-mouse secondary antibody	872:933	Afterwards, the alkaline phosphatase labeled anti-mouse secondary antibody was bound to the electrode surface by reacting with the primary antibody.					
30563124	3	69	theme	AuNPs/cSWNTs/CS	555:569	arg1	composite					572:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite	463:580	In our work, a specific electrochemical immunosensor based on gold nanoparticles/carboxylic group-functionalized single-walled carbon nanotubes/chitosan (AuNPs/cSWNTs/CS) composite was established.					
30563124	8	70	theme	μg·L-1	1366:1371	arg1	limit					1352:1356	a detection limit	1340:1356	a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%	1340:1408	Under optimal conditions, this method could quantitatively detect T-2 from 0.01 to 100 μg·L-1 with a detection limit of 0.13 μg·L-1 and favorable recovery 91.42⁻102.49%.					
30563124	4	71	theme	competitive	665:675	arg1	mechanism					603:611	The mechanism	599:611	The mechanism of the electrochemical immunosensor	599:647	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
30563124	4	71	theme	competitive	665:675	arg1	binding					677:683	an indirect competitive binding	653:683	an indirect competitive binding to a given amount of anti-T-2	653:713	The mechanism of the electrochemical immunosensor was an indirect competitive binding to a given amount of anti-T-2 between free T-2 and T-2-bovine serum albumin, which was conjugated on covalently functionalized cSWNTs decorated on the glass carbon electrode.					
31760223	8	0	theme	NIR	2157:2159	arg1	irradiation					2161:2171	NIR irradiation	2157:2171	NIR irradiation	2157:2171	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	4	1	theme	final	1100:1104	arg1	effects					1106:1112	the final effects	1096:1112	the final effects of bone tissue repair	1096:1134	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	3	2	theme	M2	731:732	arg1	"					754:754	"M2 polarization inducer"	730:754	"M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence	730:848	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	3	3	theme	cell	588:591	arg1	membranes					593:601	lipopolysaccharide-treated macrophage cell membranes	550:601	lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC)	550:657	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	1	4	theme	bone	298:301	arg1	repair					310:315	bone tissue repair	298:315	bone tissue repair	298:315	Controlling of pro-inflammation induced by pro-inflammatory cytokines and anti-inflammatory response induced by M2 macrophages is important for osteogenesis in the process of bone tissue repair.					
31760223	3	5	theme	lipopolysaccharide-treated	550:575	arg1	membranes					593:601	lipopolysaccharide-treated macrophage cell membranes	550:601	lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC)	550:657	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	4	6	dep	sequence	1207:1214	arg1	accord					1219:1224	accord	1219:1224	accord with the in vitro investigation	1219:1256	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	1	7	theme	anti-inflammatory	197:213	arg1	response					215:222	anti-inflammatory response	197:222	anti-inflammatory response	197:222	Controlling of pro-inflammation induced by pro-inflammatory cytokines and anti-inflammatory response induced by M2 macrophages is important for osteogenesis in the process of bone tissue repair.					
31760223	1	8	theme	tissue	303:308	arg1	repair					310:315	bone tissue repair	298:315	bone tissue repair	298:315	Controlling of pro-inflammation induced by pro-inflammatory cytokines and anti-inflammatory response induced by M2 macrophages is important for osteogenesis in the process of bone tissue repair.					
31760223	4	9	theme	M2	1188:1189	arg1	polarization					1191:1202	M2 polarization	1188:1202	M2 polarization	1188:1202	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	3	10	theme	inducer	747:753	arg1	"					754:754	"M2 polarization inducer"	730:754	"M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence	730:848	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	6	11	theme	anti-inflammatory	1616:1632	arg1	effects					1634:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	1	12	theme	M2	235:236	arg1	macrophages					238:248	M2 macrophages	235:248	M2 macrophages	235:248	Controlling of pro-inflammation induced by pro-inflammatory cytokines and anti-inflammatory response induced by M2 macrophages is important for osteogenesis in the process of bone tissue repair.					
31760223	7	13	theme	infection	1767:1775	arg1	diseases					1777:1784	some infection diseases	1762:1784	some infection diseases	1762:1784	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	4	14	theme	in	956:957	arg1	application					964:974	in vivo application	956:974	in vivo application of femoral bone defects	956:998	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	4	15	theme	inflammatory	1155:1166	arg1	response					1168:1175	inflammatory response	1155:1175	inflammatory response	1155:1175	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	1	16	from	process	287:293	arg1	important					253:261	important	253:261	important	253:261	Controlling of pro-inflammation induced by pro-inflammatory cytokines and anti-inflammatory response induced by M2 macrophages is important for osteogenesis in the process of bone tissue repair.					
31760223	8	17	theme	Anti-inflammatory	1882:1898	arg1	Nano-Capsule					1900:1911	Biomimetic Anti-inflammatory Nano-Capsule	1871:1911	Biomimetic Anti-inflammatory Nano-Capsule (BANC)	1871:1918	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	8	17	theme	Anti-inflammatory	1882:1898	arg1	BANC					1914:1917	BANC	1914:1917	BANC	1914:1917	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	9	18	dep	cytokines	2305:2313	arg1	blocker					2315:2321	blocker	2315:2321	blocker	2315:2321	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	9	18	dep	cytokines	2305:2313	arg1	inducer					2343:2349	M2 polarization inducer	2327:2349	M2 polarization inducer	2327:2349	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	9	18	dep	cytokines	2305:2313	arg1	cytokines					2305:2313	cytokines	2305:2313	cytokines blocker and M2 polarization inducer	2305:2349	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	7	19	from	role	1797:1800	arg1	process					1816:1822	biological process	1805:1822	biological process of bone repair	1805:1837	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	8	20	theme	Resolvin	2098:2105	arg1	inside					2110:2115	Resolvin D1 inside	2098:2115	Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair	2098:2276	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	3	21	theme	gold	638:641	arg1	nanocage					643:650	enveloping gold nanocage	627:650	cytokine receptors enveloping gold nanocage (AuNC)	608:657	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	3	21	theme	gold	638:641	arg1	AuNC					653:656	AuNC	653:656	AuNC	653:656	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	7	22	theme	cell	1743:1746	arg1	responses					1748:1756	natural cell responses	1735:1756	natural cell responses for some infection diseases	1735:1784	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	4	23	theme	in	1235:1236	arg1	investigation					1244:1256	the in vitro investigation	1231:1256	the in vitro investigation	1231:1256	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	9	24	theme	bone	2366:2369	arg1	regeneration					2371:2382	the bone regeneration	2362:2382	the bone regeneration	2362:2382	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	3	25	theme	chronological	861:873	arg1	events					875:880	these chronological events	855:880	these chronological events	855:880	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	4	26	theme	defects	992:998	arg1	application					964:974	in vivo application	956:974	in vivo application of femoral bone defects	956:998	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	3	27	theme	near-infrared	806:818	arg1	irradiation					826:836	near-infrared laser irradiation	806:836	near-infrared laser irradiation in sequence	806:848	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	6	28	theme	potential	1447:1455	arg1	strategies					1469:1478	potential therapeutic strategies	1447:1478	potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects	1447:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	3	29	theme	healing	907:913	arg1	process					915:921	the healing process	903:921	the healing process of bone tissue repair	903:943	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	8	30	from	receptors	1962:1970	arg1	surface					1979:1985	the surface	1975:1985	the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair	1975:2276	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	0	31	theme	polarization	78:89	arg1	inducer					91:97	M2 polarization inducer	75:97	M2 polarization inducer	75:97	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	8	32	theme	cell	1933:1936	arg1	membrane					1938:1945	cell membrane	1933:1945	cell membrane	1933:1945	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	3	33	dep	receptors	617:625	arg1	nanocage					643:650	enveloping gold nanocage	627:650	cytokine receptors enveloping gold nanocage (AuNC)	608:657	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	3	33	dep	receptors	617:625	arg1	AuNC					653:656	AuNC	653:656	AuNC	653:656	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	7	34	theme	repair	1832:1837	arg1	process					1816:1822	biological process	1805:1822	biological process of bone repair	1805:1837	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	9	35	theme	promising	2403:2411	arg1	potential					2413:2421	a promising potential	2401:2421	a promising potential for the treatment of bone repair and regeneration	2401:2471	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	3	36	theme	tissue	931:936	arg1	repair					938:943	bone tissue repair	926:943	bone tissue repair	926:943	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	7	37	theme	membrane-mimic	1675:1688	arg1	nanomaterials					1690:1702	Cell membrane-mimic nanomaterials	1670:1702	Cell membrane-mimic nanomaterials	1670:1702	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	4	38	theme	femoral	979:985	arg1	defects					992:998	femoral bone defects	979:998	femoral bone defects	979:998	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	5	39	theme	biomimetic	1377:1386	arg1	repair					1365:1370	the bone tissue repair	1349:1370	the bone tissue repair	1349:1370	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	5	39	theme	biomimetic	1377:1386	arg1	effector					1406:1413	a biomimetic anti-inflammation effector	1375:1413	a biomimetic anti-inflammation effector	1375:1413	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	2	40	theme	M2	424:425	arg1	polarization					438:449	M2 macrophage polarization	424:449	M2 macrophage polarization	424:449	Thus, we fabricated biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair.					
31760223	0	41	theme	tissue	108:113	arg1	repair					115:120	bone tissue repair	103:120	bone tissue repair	103:120	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	7	42	theme	biological	1805:1814	arg1	process					1816:1822	biological process	1805:1822	biological process of bone repair	1805:1837	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	4	43	theme	bioactive	1061:1069	arg1	scaffolds					1077:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	9	44	theme	BANC	2297:2300	arg1	Administration					2279:2292	Administration	2279:2292	Administration of BANC as cytokines blocker and M2 polarization inducer	2279:2349	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	0	45	theme	Biomimetic	0:9	arg1	nano-capsule					29:40	Biomimetic anti-inflammatory nano-capsule	0:40	Biomimetic anti-inflammatory nano-capsule	0:40	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	0	45	theme	Biomimetic	0:9	arg1	blocker					63:69	a cytokine blocker	52:69	a cytokine blocker	52:69	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	3	46	dep	D1	707:708	arg1	inside					710:715	inside	710:715	inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence	710:848	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	6	47	dep	potential	1447:1455	arg1	therapeutic					1457:1467	therapeutic	1457:1467	therapeutic	1457:1467	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	3	48	dep	nanosystem	526:535	arg1	loaded					686:691	loaded	686:691	loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence	686:848	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	3	48	dep	nanosystem	526:535	arg1	coated					538:543	coated	538:543	coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker"	538:679	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	8	49	theme	bone	2229:2232	arg1	formation					2234:2242	the following bone formation	2215:2242	the following bone formation	2215:2242	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	2	50	theme	positive	465:472	arg1	role					474:477	a positive role	463:477	a positive role for bone tissue repair	463:500	Thus, we fabricated biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair.					
31760223	5	51	theme	tissue	1358:1363	arg1	repair					1365:1370	the bone tissue repair	1349:1370	the bone tissue repair	1349:1370	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	5	51	theme	tissue	1358:1363	arg1	effector					1406:1413	a biomimetic anti-inflammation effector	1375:1413	a biomimetic anti-inflammation effector	1375:1413	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	9	52	theme	bone	2444:2447	arg1	repair					2449:2454	bone repair	2444:2454	bone repair	2444:2454	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	6	53	theme	inflammation-related	1503:1522	arg1	defects					1529:1535	trauma-mediated or inflammation-related bone defects	1484:1535	trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects	1484:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	7	54	theme	SIGNIFICANCE	1656:1667	arg1	STATEMENT					1643:1651	STATEMENT	1643:1651	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.	1643:1849	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	4	55	theme	BANC	1018:1021	arg1	scaffolds					1077:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	6	56	theme	trauma-mediated	1484:1498	arg1	defects					1529:1535	trauma-mediated or inflammation-related bone defects	1484:1535	trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects	1484:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	8	57	theme	M2	2184:2185	arg1	polarization					2198:2209	M2 macrophage polarization	2184:2209	M2 macrophage polarization for the following bone formation	2184:2242	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	4	58	theme	boron-containing	1033:1048	arg1	scaffolds					1077:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	3	59	theme	blocker	672:678	arg1	"					679:679	"cytokine blocker"	662:679	"cytokine blocker"	662:679	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	0	60	theme	cytokine	54:61	arg1	nano-capsule					29:40	Biomimetic anti-inflammatory nano-capsule	0:40	Biomimetic anti-inflammatory nano-capsule	0:40	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	0	60	theme	cytokine	54:61	arg1	blocker					63:69	a cytokine blocker	52:69	a cytokine blocker	52:69	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	8	61	theme	bone	2266:2269	arg1	repair					2271:2276	bone repair	2266:2276	bone repair	2266:2276	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	4	62	theme	bone	1117:1120	arg1	repair					1129:1134	bone tissue repair	1117:1134	bone tissue repair	1117:1134	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	3	63	from	irradiation	826:836	arg1	sequence					841:848	sequence	841:848	sequence	841:848	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	3	64	theme	resolvin	698:705	arg1	D1					707:708	resolvin D1	698:708	resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence	698:848	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	6	65	theme	pro-inflammatory	1586:1601	arg1	effects					1634:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	5	66	theme	cytokine	1266:1273	arg1	neutralization					1275:1288	cytokine neutralization	1266:1288	cytokine neutralization	1266:1288	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	4	67	theme	repair	1129:1134	arg1	effects					1106:1112	the final effects	1096:1112	the final effects of bone tissue repair	1096:1134	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	9	68	theme	regeneration	2460:2471	arg1	treatment					2431:2439	the treatment	2427:2439	the treatment of bone repair and regeneration	2427:2471	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	2	69	theme	tissue	488:493	arg1	repair					495:500	bone tissue repair	483:500	bone tissue repair	483:500	Thus, we fabricated biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair.					
31760223	3	70	theme	biomimic	517:524	arg1	nanosystem					526:535	a biomimic nanosystem	515:535	a biomimic nanosystem	515:535	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	3	70	theme	biomimic	517:524	arg1	BANC					507:510	The BANC	503:510	The BANC	503:510	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	6	71	theme	biomimetic	1548:1557	arg1	nanomaterial					1559:1570	a biomimetic nanomaterial	1546:1570	a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects	1546:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	3	72	theme	macrophage	577:586	arg1	membranes					593:601	lipopolysaccharide-treated macrophage cell membranes	550:601	lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC)	550:657	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	2	73	theme	biomimetic	338:347	arg1	BANC					381:384	BANC	381:384	BANC	381:384	Thus, we fabricated biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair.					
31760223	2	73	theme	biomimetic	338:347	arg1	nano-capsule					367:378	biomimetic anti-inflammatory nano-capsule	338:378	biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair	338:500	Thus, we fabricated biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair.					
31760223	3	74	theme	polarization	734:745	arg1	"					754:754	"M2 polarization inducer"	730:754	"M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence	730:848	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	8	75	theme	pro-inflammatory	2014:2029	arg1	cytokine					2031:2038	the pro-inflammatory cytokine	2010:2038	the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair	2010:2276	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	2	76	theme	bone	483:486	arg1	repair					495:500	bone tissue repair	483:500	bone tissue repair	483:500	Thus, we fabricated biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair.					
31760223	6	77	with	nanomaterial	1559:1570	arg1	effects					1634:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	3	78	theme	cytokine	608:615	arg1	receptors					617:625	cytokine receptors enveloping gold nanocage (AuNC)	608:657	cytokine receptors enveloping gold nanocage (AuNC)	608:657	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	8	79	theme	D1	2107:2108	arg1	inside					2110:2115	Resolvin D1 inside	2098:2115	Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair	2098:2276	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	4	80	dep	in	1235:1236	arg1	vitro					1238:1242	vitro	1238:1242	vitro	1238:1242	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	9	81	theme	polarization	2330:2341	arg1	inducer					2343:2349	M2 polarization inducer	2327:2349	M2 polarization inducer	2327:2349	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	9	81	theme	polarization	2330:2341	arg1	cytokines					2305:2313	cytokines	2305:2313	cytokines blocker and M2 polarization inducer	2305:2349	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	6	82	theme	enhanced	1607:1614	arg1	effects					1634:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	4	83	dep	in	956:957	arg1	vivo					959:962	vivo	959:962	vivo	959:962	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	3	84	theme	enveloping	627:636	arg1	nanocage					643:650	enveloping gold nanocage	627:650	cytokine receptors enveloping gold nanocage (AuNC)	608:657	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	3	84	theme	enveloping	627:636	arg1	AuNC					653:656	AuNC	653:656	AuNC	653:656	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	8	85	theme	Biomimetic	1871:1880	arg1	Nano-Capsule					1900:1911	Biomimetic Anti-inflammatory Nano-Capsule	1871:1911	Biomimetic Anti-inflammatory Nano-Capsule (BANC)	1871:1918	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	8	85	theme	Biomimetic	1871:1880	arg1	BANC					1914:1917	BANC	1914:1917	BANC	1914:1917	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	7	86	theme	natural	1735:1741	arg1	responses					1748:1756	natural cell responses	1735:1756	natural cell responses for some infection diseases	1735:1784	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	1	87	from	important	253:261	arg1	process					287:293	the process	283:293	the process of bone tissue repair	283:315	Controlling of pro-inflammation induced by pro-inflammatory cytokines and anti-inflammatory response induced by M2 macrophages is important for osteogenesis in the process of bone tissue repair.					
31760223	0	88	theme	M2	75:76	arg1	inducer					91:97	M2 polarization inducer	75:97	M2 polarization inducer	75:97	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	4	89	theme	bone	987:990	arg1	defects					992:998	femoral bone defects	979:998	femoral bone defects	979:998	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	7	90	dep	STATEMENT	1643:1651	arg1	unknown					1842:1848	unknown	1842:1848	unknown	1842:1848	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	7	90	dep	STATEMENT	1643:1651	arg1	popular					1714:1720	popular	1714:1720	popular	1714:1720	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	5	91	theme	macrophage	1297:1306	arg1	polarization					1308:1319	M2 macrophage polarization	1294:1319	M2 macrophage polarization	1294:1319	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	3	92	theme	laser	820:824	arg1	irradiation					826:836	near-infrared laser irradiation	806:836	near-infrared laser irradiation in sequence	806:848	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	9	93	theme	M2	2327:2328	arg1	inducer					2343:2349	M2 polarization inducer	2327:2349	M2 polarization inducer	2327:2349	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	9	93	theme	M2	2327:2328	arg1	cytokines					2305:2313	cytokines	2305:2313	cytokines blocker and M2 polarization inducer	2305:2349	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	1	94	theme	pro-inflammatory	166:181	arg1	cytokines					183:191	pro-inflammatory cytokines	166:191	pro-inflammatory cytokines	166:191	Controlling of pro-inflammation induced by pro-inflammatory cytokines and anti-inflammatory response induced by M2 macrophages is important for osteogenesis in the process of bone tissue repair.					
31760223	0	95	theme	bone	103:106	arg1	repair					115:120	bone tissue repair	103:120	bone tissue repair	103:120	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	7	96	theme	Cell	1670:1673	arg1	nanomaterials					1690:1702	Cell membrane-mimic nanomaterials	1670:1702	Cell membrane-mimic nanomaterials	1670:1702	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	8	97	theme	activated	2058:2066	arg1	pro-inflammation					2068:2083	activated pro-inflammation	2058:2083	activated pro-inflammation	2058:2083	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	3	98	theme	repair	938:943	arg1	process					915:921	the healing process	903:921	the healing process of bone tissue repair	903:943	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	8	99	theme	following	2219:2227	arg1	formation					2234:2242	the following bone formation	2215:2242	the following bone formation	2215:2242	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	5	100	theme	anti-inflammation	1388:1404	arg1	repair					1365:1370	the bone tissue repair	1349:1370	the bone tissue repair	1349:1370	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	5	100	theme	anti-inflammation	1388:1404	arg1	effector					1406:1413	a biomimetic anti-inflammation effector	1375:1413	a biomimetic anti-inflammation effector	1375:1413	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	0	101	theme	anti-inflammatory	11:27	arg1	nano-capsule					29:40	Biomimetic anti-inflammatory nano-capsule	0:40	Biomimetic anti-inflammatory nano-capsule	0:40	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	0	101	theme	anti-inflammatory	11:27	arg1	blocker					63:69	a cytokine blocker	52:69	a cytokine blocker	52:69	Biomimetic anti-inflammatory nano-capsule serves as a cytokine blocker and M2 polarization inducer for bone tissue repair.					
31760223	3	102	with	consistent	887:896	arg1	process					915:921	the healing process	903:921	the healing process of bone tissue repair	903:943	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	8	103	theme	cytokines	1952:1960	arg1	receptors					1962:1970	cytokines receptors	1952:1970	cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair	1952:2276	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	3	104	theme	bone	926:929	arg1	repair					938:943	bone tissue repair	926:943	bone tissue repair	926:943	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	4	105	theme	mesoporous	1050:1059	arg1	scaffolds					1077:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	5	106	theme	bone	1353:1356	arg1	repair					1365:1370	the bone tissue repair	1349:1370	the bone tissue repair	1349:1370	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	5	106	theme	bone	1353:1356	arg1	effector					1406:1413	a biomimetic anti-inflammation effector	1375:1413	a biomimetic anti-inflammation effector	1375:1413	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
31760223	6	107	theme	bone	1524:1527	arg1	defects					1529:1535	trauma-mediated or inflammation-related bone defects	1484:1535	trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects	1484:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	1	108	theme	repair	310:315	arg1	process					287:293	the process	283:293	the process of bone tissue repair	283:315	Controlling of pro-inflammation induced by pro-inflammatory cytokines and anti-inflammatory response induced by M2 macrophages is important for osteogenesis in the process of bone tissue repair.					
31760223	4	109	theme	glass	1071:1075	arg1	scaffolds					1077:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	9	110	theme	repair	2449:2454	arg1	treatment					2431:2439	the treatment	2427:2439	the treatment of bone repair and regeneration	2427:2471	Administration of BANC as cytokines blocker and M2 polarization inducer to enhance the bone regeneration, thus presenting a promising potential for the treatment of bone repair and regeneration.					
31760223	8	111	theme	macrophage	2187:2196	arg1	polarization					2198:2209	M2 macrophage polarization	2184:2209	M2 macrophage polarization for the following bone formation	2184:2242	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	7	112	theme	bone	1827:1830	arg1	repair					1832:1837	bone repair	1827:1837	bone repair	1827:1837	STATEMENT OF SIGNIFICANCE: Cell membrane-mimic nanomaterials have been popular for blocking natural cell responses for some infection diseases, yet their role in biological process of bone repair is unknown.					
31760223	3	113	theme	cytokine	663:670	arg1	"					679:679	"cytokine blocker"	662:679	"cytokine blocker"	662:679	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	2	114	theme	macrophage	427:436	arg1	polarization					438:449	M2 macrophage polarization	424:449	M2 macrophage polarization	424:449	Thus, we fabricated biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair.					
31760223	8	115	theme	repair	2271:2276	arg1	process					2255:2261	the process	2251:2261	the process of bone repair	2251:2276	Here, we fabricated Biomimetic Anti-inflammatory Nano-Capsule (BANC), coated with cell membrane with cytokines receptors on the surface which could neutralize the pro-inflammatory cytokine receptor to block activated pro-inflammation, loaded with Resolvin D1 inside which could be controllably released by NIR irradiation to promote M2 macrophage polarization for the following bone formation during the process of bone repair.					
31760223	4	116	theme	composite	1023:1031	arg1	scaffolds					1077:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	the BANC composite boron-containing mesoporous bioactive glass scaffolds	1014:1085	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	2	117	theme	anti-inflammatory	349:365	arg1	BANC					381:384	BANC	381:384	BANC	381:384	Thus, we fabricated biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair.					
31760223	2	117	theme	anti-inflammatory	349:365	arg1	nano-capsule					367:378	biomimetic anti-inflammatory nano-capsule	338:378	biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair	338:500	Thus, we fabricated biomimetic anti-inflammatory nano-capsule (BANC) that can block cytokines and promote M2 macrophage polarization, presenting a positive role for bone tissue repair.					
31760223	6	118	theme	weakened	1577:1584	arg1	effects					1634:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	weakened pro-inflammatory and enhanced anti-inflammatory effects	1577:1640	Therefore, this study provides potential therapeutic strategies for trauma-mediated or inflammation-related bone defects based on a biomimetic nanomaterial with weakened pro-inflammatory and enhanced anti-inflammatory effects.					
31760223	4	119	theme	tissue	1122:1127	arg1	repair					1129:1134	bone tissue repair	1117:1134	bone tissue repair	1117:1134	Moreover, in vivo application of femoral bone defects revealed that the BANC composite boron-containing mesoporous bioactive glass scaffolds improved the final effects of bone tissue repair through preventing inflammatory response, promoting M2 polarization in sequence in accord with the in vitro investigation.					
31760223	3	120	with	membranes	593:601	arg1	receptors					617:625	cytokine receptors enveloping gold nanocage (AuNC)	608:657	cytokine receptors enveloping gold nanocage (AuNC)	608:657	The BANC is a biomimic nanosystem, coated with lipopolysaccharide-treated macrophage cell membranes with cytokine receptors enveloping gold nanocage (AuNC) as "cytokine blocker", and loaded with resolvin D1 inside into AuNC as "M2 polarization inducer" whose controlled-release could be triggered under near-infrared laser irradiation in sequence, and these chronological events were consistent with the healing process of bone tissue repair.					
31760223	5	121	theme	M2	1294:1295	arg1	polarization					1308:1319	M2 macrophage polarization	1294:1319	M2 macrophage polarization	1294:1319	Hence, cytokine neutralization and M2 macrophage polarization enables the BANC to enhance the bone tissue repair as a biomimetic anti-inflammation effector.					
30031352	3	0	located	observed	1069:1076	arg1	carp					1093:1096	fresh grass carp	1081:1096	fresh grass carp	1081:1096	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	3	0	located	observed	1069:1076	arg2	family					1019:1024	the bacteria family	1006:1024	the bacteria family of Lachnospiraceae and Bacteroidaceae	1006:1062	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	3	0	located	observed	1069:1076	arg2	phyla					949:953	the major bacteria phyla	930:953	the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes	930:1000	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	1	1	theme	high-throughput	482:496	arg1	sequencing					498:507	high-throughput sequencing	482:507	high-throughput sequencing	482:507	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	2	theme	Ctenopharyngodon	236:251	arg1	carp					230:233	grass carp	224:233	grass carp (Ctenopharyngodon idellus) fillets	224:268	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	2	theme	Ctenopharyngodon	236:251	arg1	idellus					253:259	Ctenopharyngodon idellus	236:259	Ctenopharyngodon idellus	236:259	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	3	theme	pH	335:336	arg1	terms					326:330	terms	326:330	terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing	326:507	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	2	4	theme	chitosan-based	562:575	arg1	coating					577:583	chitosan-based coating	562:583	chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage	562:884	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	4	5	theme	storage	1102:1108	arg1	time					1110:1113	storage time	1102:1113	storage time	1102:1113	As storage time increased, the coated samples retained bacterial diversity.					
30031352	6	6	theme	fillets	1607:1613	arg1	storage					1585:1591	refrigerated storage	1572:1591	refrigerated storage of grass carp fillets	1572:1613	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	5	7	theme	spoiled	1290:1296	arg1	samples					1306:1312	spoiled control samples	1290:1312	spoiled control samples	1290:1312	However, Shewanellaceae, Pseudomonadaceae and Flavobacteriaceae increased and became the predominant microbiota in spoiled control samples.					
30031352	3	8	theme	high-throughput	893:907	arg1	sequencing					909:918	high-throughput sequencing	893:918	high-throughput sequencing analysis	893:927	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	3	9	theme	sequencing	909:918	arg1	analysis					920:927	high-throughput sequencing analysis	893:927	high-throughput sequencing analysis	893:927	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	1	10	theme	adenosine	399:407	arg1	triphosphate					409:420	adenosine triphosphate	399:420	adenosine triphosphate (ATP)-related compounds	399:444	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	10	theme	adenosine	399:407	arg1	ATP					423:425	ATP	423:425	ATP	423:425	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	2	11	theme	TVB-N	674:678	arg1	values					664:669	lower values	658:669	lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx)	658:731	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	11	theme	TVB-N	674:678	arg1	value					739:743	K value	737:743	K value	737:743	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	6	12	contain	had	1429:1431	arg1	coating					1421:1427	the coating	1417:1427	the coating	1417:1427	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	6	12	contain	had	1429:1431	arg2	potential					1437:1445	the potential to inhibit microbial growth, especially spoilage microorganisms	1433:1509	the potential to inhibit microbial growth, especially spoilage microorganisms	1433:1509	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	1	13	theme	total	339:343	arg1	TVB-N					369:373	TVB-N	369:373	TVB-N	369:373	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	13	theme	total	339:343	arg1	nitrogen					359:366	total volatile base nitrogen	339:366	total volatile base nitrogen (TVB-N)	339:374	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	6	14	theme	control	1378:1384	arg1	samples					1397:1403	the control and coated samples	1374:1403	samples	1397:1403	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	1	15	theme	triphosphate	409:420	arg1	compounds					436:444	adenosine triphosphate (ATP)-related compounds	399:444	adenosine triphosphate (ATP)-related compounds	399:444	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	16	theme	sequencing	498:507	arg1	terms					326:330	terms	326:330	terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing	326:507	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	17	theme	fillets	262:268	arg1	spoilage					212:219	microbial spoilage	202:219	microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets	202:268	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	3	18	theme	Bacteroidaceae	1049:1062	arg1	family					1019:1024	the bacteria family	1006:1024	the bacteria family of Lachnospiraceae and Bacteroidaceae	1006:1062	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	3	18	theme	Bacteroidaceae	1049:1062	arg1	phyla					949:953	the major bacteria phyla	930:953	the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes	930:1000	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	1	19	theme	volatile	345:352	arg1	TVB-N					369:373	TVB-N	369:373	TVB-N	369:373	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	19	theme	volatile	345:352	arg1	nitrogen					359:366	total volatile base nitrogen	339:366	total volatile base nitrogen (TVB-N)	339:374	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	2	20	theme	%	602:602	arg1	monolaurate					613:623	0.3% glycerol monolaurate	599:623	0.3% glycerol monolaurate	599:623	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	1	21	from	effects	145:151	arg1	inhibition					188:197	the inhibition	184:197	the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets	184:268	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	3	22	theme	Lachnospiraceae	1029:1043	arg1	family					1019:1024	the bacteria family	1006:1024	the bacteria family of Lachnospiraceae and Bacteroidaceae	1006:1062	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	3	22	theme	Lachnospiraceae	1029:1043	arg1	phyla					949:953	the major bacteria phyla	930:953	the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes	930:1000	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	1	23	theme	chitosan-based	158:171	arg1	coating					173:179	a chitosan-based coating	156:179	a chitosan-based coating	156:179	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	0	24	theme	chitosan-based	89:102	arg1	coating					104:110	a chitosan-based coating	87:110	a chitosan-based coating during refrigerated storage	87:138	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets with a chitosan-based coating during refrigerated storage.					
30031352	1	25	theme	base	354:357	arg1	TVB-N					369:373	TVB-N	369:373	TVB-N	369:373	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	25	theme	base	354:357	arg1	nitrogen					359:366	total volatile base nitrogen	339:366	total volatile base nitrogen (TVB-N)	339:374	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	2	26	theme	0.3	599:601	arg1	%					602:602	%	602:602	%	602:602	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	6	27	theme	significant	1319:1329	arg1	difference					1331:1340	The significant difference	1315:1340	The significant difference between the bacteria species in the control and coated samples	1315:1403	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	1	28	theme	coating	173:179	arg1	effects					145:151	The effects	141:151	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets	141:268	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	6	29	theme	bacteria	1354:1361	arg1	species					1363:1369	the bacteria species	1350:1369	the bacteria species in the control and coated samples	1350:1403	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	1	30	theme	nitrogen	359:366	arg1	terms					326:330	terms	326:330	terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing	326:507	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	3	31	theme	major	934:938	arg1	phyla					949:953	the major bacteria phyla	930:953	the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes	930:1000	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	2	32	theme	essential	640:648	arg1	oil					650:652	essential oil	640:652	essential oil	640:652	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	33	theme	hypoxanthine	686:697	arg1	HxR					709:711	HxR	709:711	HxR	709:711	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	33	theme	hypoxanthine	686:697	arg1	riboside					699:706	hypoxanthine riboside	686:706	hypoxanthine riboside (HxR)	686:712	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	33	theme	hypoxanthine	686:697	arg1	TVB-N					674:678	TVB-N	674:678	TVB-N	674:678	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	3	34	theme	bacteria	940:947	arg1	phyla					949:953	the major bacteria phyla	930:953	the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes	930:1000	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	1	35	theme	-related	427:434	arg1	compounds					436:444	adenosine triphosphate (ATP)-related compounds	399:444	adenosine triphosphate (ATP)-related compounds	399:444	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	6	36	theme	carp	1602:1605	arg1	fillets					1607:1613	grass carp fillets	1596:1613	grass carp fillets	1596:1613	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	0	37	theme	spoilage	24:31	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus)	0:72	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets with a chitosan-based coating during refrigerated storage.					
30031352	2	38	theme	clove	634:638	arg1	%					632:632	0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage	629:884	0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage	629:884	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	0	39	theme	refrigerated	119:130	arg1	storage					132:138	refrigerated storage	119:138	refrigerated storage	119:138	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets with a chitosan-based coating during refrigerated storage.					
30031352	1	40	theme	compounds	436:444	arg1	terms					326:330	terms	326:330	terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing	326:507	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	2	41	theme	glycerol	604:611	arg1	monolaurate					613:623	0.3% glycerol monolaurate	599:623	0.3% glycerol monolaurate	599:623	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	6	42	theme	refrigerated	1572:1583	arg1	storage					1585:1591	refrigerated storage	1572:1591	refrigerated storage of grass carp fillets	1572:1613	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	0	43	theme	microbial	14:22	arg1	spoilage					24:31	microbial spoilage	14:31	microbial spoilage of grass carp (Ctenopharyngodon idellus)	14:72	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets with a chitosan-based coating during refrigerated storage.					
30031352	2	44	theme	lower	658:662	arg1	values					664:669	lower values	658:669	lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx)	658:731	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	4	45	theme	bacterial	1154:1162	arg1	diversity					1164:1172	bacterial diversity	1154:1172	bacterial diversity	1154:1172	As storage time increased, the coated samples retained bacterial diversity.					
30031352	0	46	theme	grass	36:40	arg1	carp					42:45	grass carp	36:45	grass carp (Ctenopharyngodon idellus)	36:72	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets with a chitosan-based coating during refrigerated storage.					
30031352	0	46	theme	grass	36:40	arg1	idellus					65:71	Ctenopharyngodon idellus	48:71	Ctenopharyngodon idellus	48:71	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets with a chitosan-based coating during refrigerated storage.					
30031352	1	47	theme	refrigerated	290:301	arg1	storage					303:309	refrigerated storage	290:309	refrigerated storage	290:309	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	3	48	theme	Firmicutes	958:967	arg1	family					1019:1024	the bacteria family	1006:1024	the bacteria family of Lachnospiraceae and Bacteroidaceae	1006:1062	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	3	48	theme	Firmicutes	958:967	arg1	phyla					949:953	the major bacteria phyla	930:953	the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes	930:1000	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	1	49	theme	microbial	202:210	arg1	spoilage					212:219	microbial spoilage	202:219	microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets	202:268	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	2	50	theme	%	819:819	arg1	P < 0.05					778:785	P < 0.05	778:785	P < 0.05	778:785	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	50	theme	%	819:819	arg1	reductions					766:775	the significant reductions	750:775	the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%	750:819	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	6	51	theme	spoilage	1487:1494	arg1	microorganisms					1496:1509	spoilage microorganisms	1487:1509	spoilage microorganisms	1487:1509	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	3	52	theme	fresh	1081:1085	arg1	carp					1093:1096	fresh grass carp	1081:1096	fresh grass carp	1081:1096	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	1	53	theme	spoilage	212:219	arg1	inhibition					188:197	the inhibition	184:197	the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets	184:268	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	2	54	dep	indicated	522:530	arg1	fillets					541:547	the fillets	537:547	the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage	537:884	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	1	55	theme	K	447:447	arg1	value					449:453	K value	447:453	K value	447:453	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	0	56	theme	carp	42:45	arg1	spoilage					24:31	microbial spoilage	14:31	microbial spoilage of grass carp (Ctenopharyngodon idellus)	14:72	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets with a chitosan-based coating during refrigerated storage.					
30031352	6	57	theme	quality	1524:1530	arg1	deterioration					1532:1544	quality deterioration	1524:1544	quality deterioration caused by bacteria	1524:1563	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	3	58	theme	bacteria	1010:1017	arg1	family					1019:1024	the bacteria family	1006:1024	the bacteria family of Lachnospiraceae and Bacteroidaceae	1006:1062	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	1	59	theme	trimethylamine	377:390	arg1	terms					326:330	terms	326:330	terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing	326:507	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	6	60	theme	grass	1596:1600	arg1	carp					1602:1605	grass carp	1596:1605	grass carp fillets	1596:1613	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	1	61	theme	value	449:453	arg1	terms					326:330	terms	326:330	terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing	326:507	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	2	62	theme	K	737:737	arg1	value					739:743	K value	737:743	K value	737:743	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	63	from	day	868:870	arg1	control					857:863	the control	853:863	the control at day 15 of storage	853:884	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	4	64	theme	coated	1130:1135	arg1	samples					1137:1143	the coated samples	1126:1143	the coated samples	1126:1143	As storage time increased, the coated samples retained bacterial diversity.					
30031352	5	65	theme	predominant	1264:1274	arg1	microbiota					1276:1285	the predominant microbiota	1260:1285	the predominant microbiota	1260:1285	However, Shewanellaceae, Pseudomonadaceae and Flavobacteriaceae increased and became the predominant microbiota in spoiled control samples.					
30031352	2	66	dep	%	632:632	arg1	had					654:656	had	654:656	had	654:656	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	5	67	theme	control	1298:1304	arg1	samples					1306:1312	spoiled control samples	1290:1312	spoiled control samples	1290:1312	However, Shewanellaceae, Pseudomonadaceae and Flavobacteriaceae increased and became the predominant microbiota in spoiled control samples.					
30031352	6	68	theme	microbial	1458:1466	arg1	growth					1468:1473	microbial growth	1458:1473	microbial growth	1458:1473	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	6	69	from	species	1363:1369	arg1	samples					1397:1403	the control and coated samples	1374:1403	samples	1397:1403	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	1	70	theme	microbial	456:464	arg1	enumeration					466:476	microbial enumeration	456:476	microbial enumeration	456:476	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	6	71	theme	coated	1390:1395	arg1	samples					1397:1403	the control and coated samples	1374:1403	samples	1397:1403	The significant difference between the bacteria species in the control and coated samples showed that the coating had the potential to inhibit microbial growth, especially spoilage microorganisms, and reduced quality deterioration caused by bacteria during refrigerated storage of grass carp fillets.					
30031352	1	72	theme	grass	224:228	arg1	carp					230:233	grass carp	224:233	grass carp (Ctenopharyngodon idellus) fillets	224:268	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	72	theme	grass	224:228	arg1	idellus					253:259	Ctenopharyngodon idellus	236:259	Ctenopharyngodon idellus	236:259	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	2	73	contain	had	654:656	arg2	values					664:669	lower values	658:669	lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx)	658:731	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	73	contain	had	654:656	arg2	value					739:743	K value	737:743	K value	737:743	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	73	contain	had	654:656	arg1	oil					650:652	essential oil	640:652	essential oil	640:652	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	74	theme	storage	878:884	arg1	day					868:870	day 15	868:873	day 15 of storage	868:884	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	1	75	theme	enumeration	466:476	arg1	terms					326:330	terms	326:330	terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing	326:507	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	1	76	theme	carp	230:233	arg1	fillets					262:268	grass carp (Ctenopharyngodon idellus) fillets	224:268	grass carp (Ctenopharyngodon idellus) fillets	224:268	The effects of a chitosan-based coating on the inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets were studied during refrigerated storage for 15 days in terms of pH, total volatile base nitrogen (TVB-N), trimethylamine (TMA), adenosine triphosphate (ATP)-related compounds, K value, microbial enumeration and high-throughput sequencing.					
30031352	0	77	theme	Ctenopharyngodon	48:63	arg1	carp					42:45	grass carp	36:45	grass carp (Ctenopharyngodon idellus)	36:72	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets with a chitosan-based coating during refrigerated storage.					
30031352	0	77	theme	Ctenopharyngodon	48:63	arg1	idellus					65:71	Ctenopharyngodon idellus	48:71	Ctenopharyngodon idellus	48:71	Inhibition of microbial spoilage of grass carp (Ctenopharyngodon idellus) fillets with a chitosan-based coating during refrigerated storage.					
30031352	3	78	theme	grass	1087:1091	arg1	carp					1093:1096	fresh grass carp	1081:1096	fresh grass carp	1081:1096	Using high-throughput sequencing analysis, the major bacteria phyla of Firmicutes, Cyanobacteria and Bacteroidetes and the bacteria family of Lachnospiraceae and Bacteroidaceae were observed in fresh grass carp.					
30031352	2	79	theme	significant	754:764	arg1	P < 0.05					778:785	P < 0.05	778:785	P < 0.05	778:785	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30031352	2	79	theme	significant	754:764	arg1	reductions					766:775	the significant reductions	750:775	the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%	750:819	The results indicated that the fillets treated with chitosan-based coating enriched with 0.3% glycerol monolaurate and 0.5% clove essential oil had lower values of TVB-N, TMA, hypoxanthine riboside (HxR), hypoxanthine (Hx) and K value with the significant reductions (P < 0.05) of nearly 34, 73, 32, 74 and 38%, respectively, when compared to the control at day 15 of storage.					
30521894	1	0	theme	genus	174:178	arg1	Aspergillus					180:190	the genus Aspergillus	170:190	the genus Aspergillus	170:190	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
30521894	0	1	theme	structural	86:95	arg1	insight					97:103	structural insight	86:103	structural insight of β-glucosidases	86:121	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.					
30521894	5	2	theme	Glycosyl	806:813	arg1	family					830:835	Glycosyl hydrolase (GH) family 1	806:837	Glycosyl hydrolase (GH) family 1 (3 gene)	806:846	Further, it was found that 29 genes, encoding β-glucosidase belong to Glycosyl hydrolase (GH) family 1 (3 gene), 3 (17 gene), 5 (4 gene), 17 (3 gene), 132 (2 gene).					
30521894	5	2	theme	Glycosyl	806:813	arg1	gene					842:845	3 gene	840:845	3 gene	840:845	Further, it was found that 29 genes, encoding β-glucosidase belong to Glycosyl hydrolase (GH) family 1 (3 gene), 3 (17 gene), 5 (4 gene), 17 (3 gene), 132 (2 gene).					
30521894	5	2	theme	Glycosyl	806:813	arg1	3					849:849	3	849:849	3	849:849	Further, it was found that 29 genes, encoding β-glucosidase belong to Glycosyl hydrolase (GH) family 1 (3 gene), 3 (17 gene), 5 (4 gene), 17 (3 gene), 132 (2 gene).					
30521894	1	3	theme	Aspergillus	180:190	arg1	Members					159:165	Members	159:165	Members of the genus Aspergillus	159:190	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
30521894	1	3	theme	Aspergillus	180:190	arg1	ascomycetes					216:226	extensively studied ascomycetes	196:226	extensively studied ascomycetes	196:226	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
30521894	0	4	theme	β-glucosidases	108:121	arg1	insight					97:103	structural insight	86:103	structural insight of β-glucosidases	86:121	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.					
30521894	8	5	theme	amino	1309:1313	arg1	acids					1315:1319	some other amino acids	1298:1319	some other amino acids i.e. Ser, Phe, Gln and Asn	1298:1346	Moreover, some other amino acids i.e. Ser, Phe, Gln and Asn are found to be involved in hydrogen bond formation and catalysis.					
30521894	4	6	theme	carbohydrate	706:717	arg1	polysaccharides					719:733	complex carbohydrate polysaccharides	698:733	complex carbohydrate polysaccharides	698:733	A total of 13,340 protein-coding genes were predicted, among them 493 are involved in degradation of complex carbohydrate polysaccharides.					
30521894	2	7	theme	Precise	330:336	arg1	assembly					351:358	Precise whole genome assembly	330:358	Precise whole genome assembly	330:358	Precise whole genome assembly and gene annotation are significant for gene functional analyses.					
30521894	4	8	theme	genes	630:634	arg1	total					599:603	A total	597:603	A total of 13,340 protein-coding genes	597:634	A total of 13,340 protein-coding genes were predicted, among them 493 are involved in degradation of complex carbohydrate polysaccharides.					
30521894	8	9	theme	bond	1385:1388	arg1	formation					1390:1398	hydrogen bond formation	1376:1398	hydrogen bond formation	1376:1398	Moreover, some other amino acids i.e. Ser, Phe, Gln and Asn are found to be involved in hydrogen bond formation and catalysis.					
30521894	3	10	dep	terreus	521:527	arg1	P14_T3					529:534	P14_T3	529:534	Aspergillus terreus P14_T3	509:534	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	6	11	theme	barrel	1098:1103	arg1	motif					1105:1109	classical (α/β) TIM-like barrel motif	1073:1109	classical (α/β) TIM-like barrel motif	1073:1109	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	2	12	theme	gene	400:403	arg1	analyses					416:423	gene functional analyses	400:423	gene functional analyses	400:423	Precise whole genome assembly and gene annotation are significant for gene functional analyses.					
30521894	9	13	theme	cellulose-hydrolyzing	1500:1520	arg1	efficiency					1522:1531	higher cellulose-hydrolyzing efficiency	1493:1531	higher cellulose-hydrolyzing efficiency	1493:1531	These findings may provide valuable insights in designing β-glucosidases with higher cellulose-hydrolyzing efficiency.					
30521894	3	14	theme	terreus	521:527	arg1	assembly					471:478	assembly	471:478	assembly	471:478	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	3	14	theme	terreus	521:527	arg1	sequencing					459:468	the draft genome sequencing	442:468	the draft genome sequencing	442:468	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	3	14	theme	terreus	521:527	arg1	analysis					497:504	whole genome analysis	484:504	whole genome analysis	484:504	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	0	15	theme	molecular	131:139	arg1	approach					149:156	molecular docking approach	131:156	molecular docking approach	131:156	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.					
30521894	7	16	theme	substrate	1266:1274	arg1	hydrolysis					1276:1285	substrate hydrolysis	1266:1285	substrate hydrolysis	1266:1285	Molecular docking of different β-glucosidases with cellobiose revealed that conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp are taking part in substrate hydrolysis.					
30521894	6	17	theme	modeled	985:991	arg1	structure					993:1001	modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17)	985:1066	modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17)	985:1066	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	6	18	theme	β-glucosidases	935:948	arg1	structure					914:922	The tertiary structure	901:922	The tertiary structure of all the β-glucosidases	901:948	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	4	19	theme	complex	698:704	arg1	polysaccharides					719:733	complex carbohydrate polysaccharides	698:733	complex carbohydrate polysaccharides	698:733	A total of 13,340 protein-coding genes were predicted, among them 493 are involved in degradation of complex carbohydrate polysaccharides.					
30521894	5	20	theme	hydrolase	815:823	arg1	family					830:835	Glycosyl hydrolase (GH) family 1	806:837	Glycosyl hydrolase (GH) family 1 (3 gene)	806:846	Further, it was found that 29 genes, encoding β-glucosidase belong to Glycosyl hydrolase (GH) family 1 (3 gene), 3 (17 gene), 5 (4 gene), 17 (3 gene), 132 (2 gene).					
30521894	5	20	theme	hydrolase	815:823	arg1	gene					842:845	3 gene	840:845	3 gene	840:845	Further, it was found that 29 genes, encoding β-glucosidase belong to Glycosyl hydrolase (GH) family 1 (3 gene), 3 (17 gene), 5 (4 gene), 17 (3 gene), 132 (2 gene).					
30521894	5	20	theme	hydrolase	815:823	arg1	3					849:849	3	849:849	3	849:849	Further, it was found that 29 genes, encoding β-glucosidase belong to Glycosyl hydrolase (GH) family 1 (3 gene), 3 (17 gene), 5 (4 gene), 17 (3 gene), 132 (2 gene).					
30521894	4	21	theme	protein-coding	615:628	arg1	genes					630:634	13,340 protein-coding genes	608:634	13,340 protein-coding genes	608:634	A total of 13,340 protein-coding genes were predicted, among them 493 are involved in degradation of complex carbohydrate polysaccharides.					
30521894	3	22	theme	draft	446:450	arg1	sequencing					459:468	the draft genome sequencing	442:468	the draft genome sequencing	442:468	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	7	23	theme	β-glucosidases	1143:1156	arg1	docking					1122:1128	Molecular docking	1112:1128	Molecular docking of different β-glucosidases with cellobiose	1112:1172	Molecular docking of different β-glucosidases with cellobiose revealed that conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp are taking part in substrate hydrolysis.					
30521894	3	24	theme	cattle	567:572	arg1	sample					557:562	rumen sample	551:562	rumen sample of cattle fed with coconut-coir	551:594	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	6	25	theme	homology	966:973	arg1	modeling					975:982	homology modeling	966:982	homology modeling	966:982	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	0	26	theme	genome	6:11	arg1	analysis					13:20	Draft genome analysis	0:20	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.	0:157	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.					
30521894	8	27	dep	Ser	1326:1328	arg1	i.e.					1321:1324	i.e.	1321:1324	i.e.	1321:1324	Moreover, some other amino acids i.e. Ser, Phe, Gln and Asn are found to be involved in hydrogen bond formation and catalysis.					
30521894	8	28	theme	hydrogen	1376:1383	arg1	formation					1390:1398	hydrogen bond formation	1376:1398	hydrogen bond formation	1376:1398	Moreover, some other amino acids i.e. Ser, Phe, Gln and Asn are found to be involved in hydrogen bond formation and catalysis.					
30521894	1	29	theme	studied	208:214	arg1	ascomycetes					216:226	extensively studied ascomycetes	196:226	extensively studied ascomycetes	196:226	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
30521894	1	29	theme	studied	208:214	arg1	Members					159:165	Members	159:165	Members of the genus Aspergillus	159:190	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
30521894	0	30	theme	Draft	0:4	arg1	analysis					13:20	Draft genome analysis	0:20	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.	0:157	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.					
30521894	1	31	theme	industrial	309:318	arg1	interest					320:327	industrial interest	309:327	industrial interest	309:327	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
30521894	9	32	theme	designing	1463:1471	arg1	β-glucosidases					1473:1486	designing β-glucosidases	1463:1486	designing β-glucosidases with higher cellulose-hydrolyzing efficiency	1463:1531	These findings may provide valuable insights in designing β-glucosidases with higher cellulose-hydrolyzing efficiency.					
30521894	6	33	theme	TIM-like	1089:1096	arg1	motif					1105:1109	classical (α/β) TIM-like barrel motif	1073:1109	classical (α/β) TIM-like barrel motif	1073:1109	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	1	34	theme	interest	320:327	arg1	enzymes					298:304	enzymes	298:304	enzymes of industrial interest	298:327	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
30521894	1	34	theme	interest	320:327	arg1	compounds					284:292	high value-added compounds	267:292	high value-added compounds	267:292	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
30521894	7	35	with	docking	1122:1128	arg1	cellobiose					1163:1172	cellobiose	1163:1172	cellobiose	1163:1172	Molecular docking of different β-glucosidases with cellobiose revealed that conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp are taking part in substrate hydrolysis.					
30521894	2	36	theme	genome	344:349	arg1	assembly					351:358	Precise whole genome assembly	330:358	Precise whole genome assembly	330:358	Precise whole genome assembly and gene annotation are significant for gene functional analyses.					
30521894	7	37	theme	different	1133:1141	arg1	β-glucosidases					1143:1156	different β-glucosidases	1133:1156	different β-glucosidases	1133:1156	Molecular docking of different β-glucosidases with cellobiose revealed that conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp are taking part in substrate hydrolysis.					
30521894	0	38	theme	docking	141:147	arg1	approach					149:156	molecular docking approach	131:156	molecular docking approach	131:156	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.					
30521894	2	39	theme	whole	338:342	arg1	assembly					351:358	Precise whole genome assembly	330:358	Precise whole genome assembly	330:358	Precise whole genome assembly and gene annotation are significant for gene functional analyses.					
30521894	4	40	theme	polysaccharides	719:733	arg1	degradation					683:693	degradation	683:693	degradation of complex carbohydrate polysaccharides	683:733	A total of 13,340 protein-coding genes were predicted, among them 493 are involved in degradation of complex carbohydrate polysaccharides.					
30521894	0	41	theme	enzymes	43:49	arg1	analysis					13:20	Draft genome analysis	0:20	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.	0:157	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.					
30521894	9	42	from	insights	1451:1458	arg1	β-glucosidases					1473:1486	designing β-glucosidases	1463:1486	designing β-glucosidases with higher cellulose-hydrolyzing efficiency	1463:1531	These findings may provide valuable insights in designing β-glucosidases with higher cellulose-hydrolyzing efficiency.					
30521894	9	43	theme	higher	1493:1498	arg1	efficiency					1522:1531	higher cellulose-hydrolyzing efficiency	1493:1531	higher cellulose-hydrolyzing efficiency	1493:1531	These findings may provide valuable insights in designing β-glucosidases with higher cellulose-hydrolyzing efficiency.					
30521894	9	44	with	β-glucosidases	1473:1486	arg1	efficiency					1522:1531	higher cellulose-hydrolyzing efficiency	1493:1531	higher cellulose-hydrolyzing efficiency	1493:1531	These findings may provide valuable insights in designing β-glucosidases with higher cellulose-hydrolyzing efficiency.					
30521894	6	45	theme	classical	1073:1081	arg1	motif					1105:1109	classical (α/β) TIM-like barrel motif	1073:1109	classical (α/β) TIM-like barrel motif	1073:1109	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	3	46	theme	whole	484:488	arg1	analysis					497:504	whole genome analysis	484:504	whole genome analysis	484:504	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	0	47	theme	lignocellulolytic	25:41	arg1	enzymes					43:49	lignocellulolytic enzymes	25:49	lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach	25:156	Draft genome analysis of lignocellulolytic enzymes producing Aspergillus terreus with structural insight of β-glucosidases through molecular docking approach.					
30521894	3	48	theme	genome	452:457	arg1	sequencing					459:468	the draft genome sequencing	442:468	the draft genome sequencing	442:468	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	6	49	theme	tertiary	905:912	arg1	structure					914:922	The tertiary structure	901:922	The tertiary structure of all the β-glucosidases	901:948	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	3	50	theme	genome	490:495	arg1	analysis					497:504	whole genome analysis	484:504	whole genome analysis	484:504	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	5	51	theme	GH	826:827	arg1	family					830:835	Glycosyl hydrolase (GH) family 1	806:837	Glycosyl hydrolase (GH) family 1 (3 gene)	806:846	Further, it was found that 29 genes, encoding β-glucosidase belong to Glycosyl hydrolase (GH) family 1 (3 gene), 3 (17 gene), 5 (4 gene), 17 (3 gene), 132 (2 gene).					
30521894	5	51	theme	GH	826:827	arg1	gene					842:845	3 gene	840:845	3 gene	840:845	Further, it was found that 29 genes, encoding β-glucosidase belong to Glycosyl hydrolase (GH) family 1 (3 gene), 3 (17 gene), 5 (4 gene), 17 (3 gene), 132 (2 gene).					
30521894	5	51	theme	GH	826:827	arg1	3					849:849	3	849:849	3	849:849	Further, it was found that 29 genes, encoding β-glucosidase belong to Glycosyl hydrolase (GH) family 1 (3 gene), 3 (17 gene), 5 (4 gene), 17 (3 gene), 132 (2 gene).					
30521894	6	52	dep	structure	993:1001	arg1	AtBgl17.1					1051:1059	AtBgl17.1	1051:1059	AtBgl17.1	1051:1059	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	6	52	dep	structure	993:1001	arg1	GH1					1013:1015	GH1	1013:1015	GH1	1013:1015	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	6	52	dep	structure	993:1001	arg1	AtBgl3.1					1019:1026	AtBgl3.1	1019:1026	AtBgl3.1	1019:1026	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	6	52	dep	structure	993:1001	arg1	AtBgl1.3					1003:1010	AtBgl1.3	1003:1010	AtBgl1.3	1003:1010	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	6	52	dep	structure	993:1001	arg1	AtBgl5.4					1035:1042	AtBgl5.4	1035:1042	AtBgl5.4	1035:1042	The tertiary structure of all the β-glucosidases was designed by homology modeling; modeled structure AtBgl1.3 (GH1), AtBgl3.1 (GH3), AtBgl5.4 (GH5), AtBgl17.1 (GH17) show classical (α/β) TIM-like barrel motif.					
30521894	7	53	theme	Molecular	1112:1120	arg1	docking					1122:1128	Molecular docking	1112:1128	Molecular docking of different β-glucosidases with cellobiose	1112:1172	Molecular docking of different β-glucosidases with cellobiose revealed that conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp are taking part in substrate hydrolysis.					
30521894	3	54	attach	isolated	537:544	arg2	terreus					521:527	Aspergillus terreus	509:527	Aspergillus terreus P14_T3	509:534	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	3	54	attach	isolated	537:544	arg1	sample					557:562	rumen sample	551:562	rumen sample of cattle fed with coconut-coir	551:594	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	9	55	theme	valuable	1442:1449	arg1	insights					1451:1458	valuable insights	1442:1458	valuable insights in designing β-glucosidases with higher cellulose-hydrolyzing efficiency	1442:1531	These findings may provide valuable insights in designing β-glucosidases with higher cellulose-hydrolyzing efficiency.					
30521894	7	56	dep	Glu	1215:1217	arg1	i.e.					1210:1213	i.e.	1210:1213	i.e.	1210:1213	Molecular docking of different β-glucosidases with cellobiose revealed that conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp are taking part in substrate hydrolysis.					
30521894	2	57	theme	gene	364:367	arg1	annotation					369:378	gene annotation	364:378	gene annotation	364:378	Precise whole genome assembly and gene annotation are significant for gene functional analyses.					
30521894	7	58	theme	conserved	1188:1196	arg1	acids					1204:1208	conserved amino acids	1188:1208	conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp	1188:1245	Molecular docking of different β-glucosidases with cellobiose revealed that conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp are taking part in substrate hydrolysis.					
30521894	3	59	theme	rumen	551:555	arg1	sample					557:562	rumen sample	551:562	rumen sample of cattle fed with coconut-coir	551:594	Here, we report the draft genome sequencing, assembly and whole genome analysis of Aspergillus terreus P14_T3, isolated from rumen sample of cattle fed with coconut-coir.					
30521894	1	60	theme	high	267:270	arg1	compounds					284:292	high value-added compounds	267:292	high value-added compounds	267:292	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
30521894	8	61	theme	other	1303:1307	arg1	acids					1315:1319	some other amino acids	1298:1319	some other amino acids i.e. Ser, Phe, Gln and Asn	1298:1346	Moreover, some other amino acids i.e. Ser, Phe, Gln and Asn are found to be involved in hydrogen bond formation and catalysis.					
30521894	7	62	theme	amino	1198:1202	arg1	acids					1204:1208	conserved amino acids	1188:1208	conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp	1188:1245	Molecular docking of different β-glucosidases with cellobiose revealed that conserved amino acids i.e. Glu, Trp, Arg, His, Tyr and Asp are taking part in substrate hydrolysis.					
30521894	2	63	theme	functional	405:414	arg1	analyses					416:423	gene functional analyses	400:423	gene functional analyses	400:423	Precise whole genome assembly and gene annotation are significant for gene functional analyses.					
30521894	1	64	theme	value-added	272:282	arg1	compounds					284:292	high value-added compounds	267:292	high value-added compounds	267:292	Members of the genus Aspergillus are extensively studied ascomycetes because of their ability to synthesize high value-added compounds and enzymes of industrial interest.					
29666989	0	0	theme	osteogenic	82:91	arg1	differentiation					93:107	the osteogenic differentiation	78:107	the osteogenic differentiation of mesenchymal stem cells	78:133	Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells.					
29666989	4	1	theme	heat	901:904	arg1	treatment					906:914	direct heat treatment	894:914	direct heat treatment applied under equal temperatures	894:947	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	4	2	theme	cells	775:779	arg1	viability					745:753	high viability	740:753	high viability of mesenchymal stem cells (MSCs)	740:786	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	5	3	theme	alternative	1072:1082	arg1	candidate					1084:1092	an alternative candidate	1069:1092	an alternative candidate in bone tissue engineering regenerative applications	1069:1145	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	5	3	theme	alternative	1072:1082	arg1	method					986:991	the nanoheat stimulation method	961:991	the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF	961:1046	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	4	4	theme	high	740:743	arg1	viability					745:753	high viability	740:753	high viability of mesenchymal stem cells (MSCs)	740:786	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	4	5	theme	elevated	797:804	arg1	temperature					806:816	the elevated temperature	793:816	the elevated temperature	793:816	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	3	6	theme	nanocomposite	379:391	arg1	hydrogel					393:400	This nanocomposite hydrogel	374:400	This nanocomposite hydrogel	374:400	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	4	7	theme	equal	930:934	arg1	temperatures					936:947	equal temperatures	930:947	equal temperatures	930:947	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	3	8	theme	increasing	598:607	arg1	temperatures					609:620	increasing temperatures	598:620	increasing temperatures	598:620	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	1	9	theme	promising	174:182	arg1	treatment					192:200	a promising healing treatment	172:200	a promising healing treatment in bone regeneration	172:221	Hyperthermia has been considered as a promising healing treatment in bone regeneration.					
29666989	1	9	theme	promising	174:182	arg1	Hyperthermia					136:147	Hyperthermia	136:147	Hyperthermia	136:147	Hyperthermia has been considered as a promising healing treatment in bone regeneration.					
29666989	0	10	theme	mesenchymal	112:122	arg1	cells					129:133	mesenchymal stem cells	112:133	mesenchymal stem cells	112:133	Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells.					
29666989	4	11	theme	highest	837:843	arg1	ability					872:878	the highest osteogenic differentiation ability	833:878	the highest osteogenic differentiation ability	833:878	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	5	12	from	candidate	1084:1092	arg1	tissue					1102:1107	bone tissue	1097:1107	bone tissue engineering regenerative applications	1097:1145	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	1	13	theme	healing	184:190	arg1	treatment					192:200	a promising healing treatment	172:200	a promising healing treatment in bone regeneration	172:221	Hyperthermia has been considered as a promising healing treatment in bone regeneration.					
29666989	1	13	theme	healing	184:190	arg1	Hyperthermia					136:147	Hyperthermia	136:147	Hyperthermia	136:147	Hyperthermia has been considered as a promising healing treatment in bone regeneration.					
29666989	5	14	theme	regenerative	1121:1132	arg1	applications					1134:1145	regenerative applications	1121:1145	regenerative applications	1121:1145	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	0	15	theme	stem	124:127	arg1	cells					129:133	mesenchymal stem cells	112:133	mesenchymal stem cells	112:133	Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells.					
29666989	3	16	theme	magnetic	447:454	arg1	nanoparticles					462:474	magnetic Fe3O4 nanoparticles	447:474	magnetic Fe3O4 nanoparticles	447:474	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	2	17	theme	hyperthermia	332:343	arg1	characteristics					313:327	the characteristics	309:327	the characteristics of hyperthermia for osteogenic regeneration	309:371	We designed a tissue engineering hydrogel based on magnetic nanoparticles to explore the characteristics of hyperthermia for osteogenic regeneration.					
29666989	5	18	theme	nanoheat	965:972	arg1	candidate					1084:1092	an alternative candidate	1069:1092	an alternative candidate in bone tissue engineering regenerative applications	1069:1145	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	5	18	theme	nanoheat	965:972	arg1	method					986:991	the nanoheat stimulation method	961:991	the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF	961:1046	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	0	19	theme	stimulation	19:29	arg1	Effect					0:5	Effect	0:5	Effect of nanoheat stimulation	0:29	Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells.					
29666989	3	20	theme	alternative	631:641	arg1	AMF					659:661	AMF	659:661	AMF	659:661	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	3	20	theme	alternative	631:641	arg1	field					652:656	an alternative magnetic field	628:656	an alternative magnetic field (AMF)	628:662	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	3	21	dep	glycol	503:508	arg1	hydrogel					516:523	hydrogel	516:523	hydrogel	516:523	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	1	22	theme	bone	205:208	arg1	regeneration					210:221	bone regeneration	205:221	bone regeneration	205:221	Hyperthermia has been considered as a promising healing treatment in bone regeneration.					
29666989	0	23	theme	nanoheat	10:17	arg1	stimulation					19:29	nanoheat stimulation	10:29	nanoheat stimulation	10:29	Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells.					
29666989	0	24	theme	cells	129:133	arg1	differentiation					93:107	the osteogenic differentiation	78:107	the osteogenic differentiation of mesenchymal stem cells	78:133	Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells.					
29666989	3	25	theme	magnetic	643:650	arg1	AMF					659:661	AMF	659:661	AMF	659:661	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	3	25	theme	magnetic	643:650	arg1	field					652:656	an alternative magnetic field	628:656	an alternative magnetic field (AMF)	628:662	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	5	26	theme	stimulation	974:984	arg1	candidate					1084:1092	an alternative candidate	1069:1092	an alternative candidate in bone tissue engineering regenerative applications	1069:1145	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	5	26	theme	stimulation	974:984	arg1	method					986:991	the nanoheat stimulation method	961:991	the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF	961:1046	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	2	27	theme	engineering	245:255	arg1	hydrogel					257:264	a tissue engineering hydrogel	236:264	a tissue engineering hydrogel based on magnetic nanoparticles	236:296	We designed a tissue engineering hydrogel based on magnetic nanoparticles to explore the characteristics of hyperthermia for osteogenic regeneration.					
29666989	3	28	theme	chitosan/polyethylene	481:501	arg1	PEG					511:513	PEG	511:513	PEG	511:513	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	3	28	theme	chitosan/polyethylene	481:501	arg1	glycol					503:508	chitosan/polyethylene glycol	481:508	chitosan/polyethylene glycol (PEG)	481:514	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	2	29	theme	tissue	238:243	arg1	hydrogel					257:264	a tissue engineering hydrogel	236:264	a tissue engineering hydrogel based on magnetic nanoparticles	236:296	We designed a tissue engineering hydrogel based on magnetic nanoparticles to explore the characteristics of hyperthermia for osteogenic regeneration.					
29666989	0	30	theme	nanocomposite	52:64	arg1	hydrogel					66:73	magnetic nanocomposite hydrogel	43:73	magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells	43:133	Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells.					
29666989	0	31	from	hydrogel	66:73	arg1	differentiation					93:107	the osteogenic differentiation	78:107	the osteogenic differentiation of mesenchymal stem cells	78:133	Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells.					
29666989	5	32	theme	magnetic	1003:1010	arg1	hydrogel					1026:1033	the magnetic nanocomposite hydrogel	999:1033	the magnetic nanocomposite hydrogel	999:1033	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	0	33	theme	magnetic	43:50	arg1	hydrogel					66:73	magnetic nanocomposite hydrogel	43:73	magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells	43:133	Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells.					
29666989	5	34	theme	nanocomposite	1012:1024	arg1	hydrogel					1026:1033	the magnetic nanocomposite hydrogel	999:1033	the magnetic nanocomposite hydrogel	999:1033	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	2	35	theme	magnetic	275:282	arg1	nanoparticles					284:296	magnetic nanoparticles	275:296	magnetic nanoparticles	275:296	We designed a tissue engineering hydrogel based on magnetic nanoparticles to explore the characteristics of hyperthermia for osteogenic regeneration.					
29666989	4	36	theme	direct	894:899	arg1	treatment					906:914	direct heat treatment	894:914	direct heat treatment applied under equal temperatures	894:947	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	3	37	theme	excellent	539:547	arg1	biocompatibility					549:564	excellent biocompatibility	539:564	excellent biocompatibility	539:564	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	4	38	theme	dispersed	680:688	arg1	nanoparticles					690:702	uniformly dispersed nanoparticles	670:702	uniformly dispersed nanoparticles	670:702	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	4	39	theme	osteogenic	845:854	arg1	ability					872:878	the highest osteogenic differentiation ability	833:878	the highest osteogenic differentiation ability	833:878	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	4	40	theme	differentiation	856:870	arg1	ability					872:878	the highest osteogenic differentiation ability	833:878	the highest osteogenic differentiation ability	833:878	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	1	41	from	treatment	192:200	arg1	regeneration					210:221	bone regeneration	205:221	bone regeneration	205:221	Hyperthermia has been considered as a promising healing treatment in bone regeneration.					
29666989	2	42	theme	osteogenic	349:358	arg1	regeneration					360:371	osteogenic regeneration	349:371	osteogenic regeneration	349:371	We designed a tissue engineering hydrogel based on magnetic nanoparticles to explore the characteristics of hyperthermia for osteogenic regeneration.					
29666989	5	43	theme	bone	1097:1100	arg1	tissue					1102:1107	bone tissue	1097:1107	bone tissue engineering regenerative applications	1097:1145	Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.					
29666989	4	44	theme	composite	709:717	arg1	hydrogel					719:726	the composite hydrogel	705:726	the composite hydrogel	705:726	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	4	45	theme	stem	770:773	arg1	cells					775:779	mesenchymal stem cells	758:779	mesenchymal stem cells (MSCs)	758:786	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	4	45	theme	stem	770:773	arg1	MSCs					782:785	MSCs	782:785	MSCs	782:785	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	3	46	theme	Fe3O4	456:460	arg1	nanoparticles					462:474	magnetic Fe3O4 nanoparticles	447:474	magnetic Fe3O4 nanoparticles	447:474	This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe3O4 nanoparticles into chitosan/polyethylene glycol (PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field (AMF).					
29666989	4	47	theme	mesenchymal	758:768	arg1	cells					775:779	mesenchymal stem cells	758:779	mesenchymal stem cells (MSCs)	758:786	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
29666989	4	47	theme	mesenchymal	758:768	arg1	MSCs					782:785	MSCs	782:785	MSCs	782:785	With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells (MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures.					
31819938	3	0	theme	ternary	584:590	arg1	collagen					572:579	collagen	572:579	collagen	572:579	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	3	0	theme	ternary	584:590	arg1	scaffold					601:608	a ternary hydrogel scaffold	582:608	a ternary hydrogel scaffold	582:608	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	9	1	theme	good	1853:1856	arg1	biocompatibility					1858:1873	good biocompatibility	1853:1873	good biocompatibility	1853:1873	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	3	2	theme	collagen	628:635	arg1	molecules					637:645	rigid collagen molecules	622:645	rigid collagen molecules	622:645	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	8	3	dep	in	1670:1671	arg1	vivo					1673:1676	vivo	1673:1676	vivo	1673:1676	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	0	4	link	Proanthocyanidin-crosslinked	0:27	arg1	hydrogel					57:64	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel	0:64	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering	0:166	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	3	5	theme	synergistic	777:787	arg1	interaction					789:799	a synergistic interaction	775:799	a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity	775:974	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	9	6	theme	biodegradation	1889:1902	arg1	integration					1838:1848	the essential integration	1824:1848	the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications	1824:2016	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	3	7	theme	mechanical	832:841	arg1	properties					843:852	the mechanical properties	828:852	the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity	828:974	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	3	8	theme	konjac	657:662	arg1	chains					682:687	flexible konjac glucomannan (KGM) chains	648:687	flexible konjac glucomannan (KGM) chains	648:687	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	9	9	theme	improved	1908:1915	arg1	properties					1928:1937	improved mechanical properties	1908:1937	improved mechanical properties	1908:1937	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	6	10	theme	Col/KGM/PA	1293:1302	arg1	implants					1304:1311	Col/KGM/PA implants	1293:1311	Col/KGM/PA implants	1293:1311	The in vivo variations of Col/KGM/PA implants are visualized in real-time by magnetic resonance imaging (MRI).					
31819938	3	11	dep	possesses	900:908	arg1	improved					933:940	improved	933:940	possesses not only substantially improved strength but also good elasticity	900:974	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	9	12	theme	properties	1928:1937	arg1	integration					1838:1848	the essential integration	1824:1848	the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications	1824:2016	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	3	13	theme	so-obtained	861:871	arg1	hydrogel					884:891	the so-obtained Col/KGM/PA hydrogel	857:891	the so-obtained Col/KGM/PA hydrogel	857:891	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	5	14	theme	KGM	1222:1224	arg1	macromolecules					1226:1239	KGM macromolecules	1222:1239	KGM macromolecules	1222:1239	TEM discovered the co-existence of two types of fibrils with distinctly different arrangement patterns, explaining the contribution of KGM macromolecules to elasticity generation.					
31819938	1	15	theme	excellent	250:258	arg1	biocompatibility					260:275	its excellent biocompatibility	246:275	its excellent biocompatibility	246:275	Collagen (Col) has been intensively exploited as a biomaterial for its excellent biocompatibility, biodegradation and bioactivity.					
31819938	7	16	theme	layer-by-layer	1520:1533	arg1	erosion					1535:1541	layer-by-layer erosion	1520:1541	layer-by-layer erosion	1520:1541	Moreover, a quantitative technique of MRI T2-mapping combined with histology is designed to visualize the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels.					
31819938	9	17	theme	desired	1956:1962	arg1	material					1973:1980	a desired scaffold material	1954:1980	a desired scaffold material for tissue engineering applications	1954:2016	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	4	18	theme	controllable	1008:1019	arg1	biodegradation					1021:1034	controllable biodegradation	1008:1034	controllable biodegradation	1008:1034	PA endows these scaffolds with controllable biodegradation and anti-calcification and antioxidant activities.					
31819938	8	19	theme	in	1646:1647	arg1	degradation					1654:1664	in vivo degradation	1646:1664	in vivo degradation	1646:1664	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	8	20	theme	degradation	1654:1664	arg1	processes					1633:1641	the respective processes	1618:1641	the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants	1618:1726	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	6	21	theme	resonance	1353:1361	arg1	imaging					1363:1369	magnetic resonance imaging	1344:1369	magnetic resonance imaging (MRI)	1344:1375	The in vivo variations of Col/KGM/PA implants are visualized in real-time by magnetic resonance imaging (MRI).					
31819938	6	21	theme	resonance	1353:1361	arg1	MRI					1372:1374	MRI	1372:1374	MRI	1372:1374	The in vivo variations of Col/KGM/PA implants are visualized in real-time by magnetic resonance imaging (MRI).					
31819938	3	22	theme	hydrogel	884:891	arg1	properties					843:852	the mechanical properties	828:852	the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity	828:974	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	9	23	theme	tissue	1986:1991	arg1	applications					2005:2016	tissue engineering applications	1986:2016	tissue engineering applications	1986:2016	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	8	24	theme	in	1670:1671	arg1	dehydration					1678:1688	in vivo dehydration	1670:1688	in vivo dehydration	1670:1688	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	2	25	dep	properties	339:348	arg1	the					319:321	the	319:321	the	319:321	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	0	26	theme	trackable	110:118	arg1	biodegradation					120:133	MRI trackable biodegradation	106:133	MRI trackable biodegradation	106:133	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	8	27	theme	dehydration	1678:1688	arg1	processes					1633:1641	the respective processes	1618:1641	the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants	1618:1726	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	2	28	theme	mechanical	328:337	arg1	properties					339:348	poor mechanical properties	323:348	poor mechanical properties	323:348	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	2	28	theme	mechanical	328:337	arg1	biodegradation					360:373	rapid biodegradation	354:373	rapid biodegradation	354:373	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	2	28	theme	mechanical	328:337	arg1	bottlenecks					432:442	the bottlenecks	428:442	the bottlenecks for their further development especially for vascular tissue engineering	428:515	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	2	29	theme	vascular	489:496	arg1	engineering					505:515	vascular tissue engineering	489:515	vascular tissue engineering	489:515	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	0	30	theme	tissue	149:154	arg1	engineering					156:166	potential tissue engineering	139:166	potential tissue engineering	139:166	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	7	31	theme	in	1484:1485	arg1	mechanism					1507:1515	the in vivo biodegradation mechanism	1480:1515	the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels	1480:1561	Moreover, a quantitative technique of MRI T2-mapping combined with histology is designed to visualize the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels.					
31819938	8	32	theme	hydrogel	1710:1717	arg1	implants					1719:1726	these controlled hydrogel implants	1693:1726	these controlled hydrogel implants	1693:1726	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	0	33	theme	Proanthocyanidin-crosslinked	0:27	arg1	hydrogel					57:64	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel	0:64	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering	0:166	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	2	34	theme	tissue	498:503	arg1	engineering					505:515	vascular tissue engineering	489:515	vascular tissue engineering	489:515	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	0	35	theme	glucomannan	45:55	arg1	hydrogel					57:64	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel	0:64	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering	0:166	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	9	36	theme	designed	1776:1783	arg1	hydrogel					1806:1813	Such a designed Col/KGM/PA composite hydrogel	1769:1813	Such a designed Col/KGM/PA composite hydrogel	1769:1813	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	3	37	theme	proanthocyanidin	723:738	arg1	chains					682:687	flexible konjac glucomannan (KGM) chains	648:687	flexible konjac glucomannan (KGM) chains	648:687	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	3	37	theme	proanthocyanidin	723:738	arg1	molecules					637:645	rigid collagen molecules	622:645	rigid collagen molecules	622:645	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	3	37	theme	proanthocyanidin	723:738	arg1	crosslinkers					707:718	biocompatible crosslinkers	693:718	biocompatible crosslinkers of proanthocyanidin (PA)	693:743	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	3	38	theme	collagen	572:579	arg1	characteristics					553:567	the self-assembly characteristics	535:567	the self-assembly characteristics	535:567	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	0	39	theme	mechanical	80:89	arg1	properties					91:100	improved mechanical properties	71:100	improved mechanical properties	71:100	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	9	40	theme	composite	1796:1804	arg1	hydrogel					1806:1813	Such a designed Col/KGM/PA composite hydrogel	1769:1813	Such a designed Col/KGM/PA composite hydrogel	1769:1813	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	6	41	theme	magnetic	1344:1351	arg1	imaging					1363:1369	magnetic resonance imaging	1344:1369	magnetic resonance imaging (MRI)	1344:1375	The in vivo variations of Col/KGM/PA implants are visualized in real-time by magnetic resonance imaging (MRI).					
31819938	6	41	theme	magnetic	1344:1351	arg1	MRI					1372:1374	MRI	1372:1374	MRI	1372:1374	The in vivo variations of Col/KGM/PA implants are visualized in real-time by magnetic resonance imaging (MRI).					
31819938	8	42	theme	different	1586:1594	arg1	relationships					1596:1608	three different relationships	1580:1608	three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants	1580:1726	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	2	43	theme	collagen	392:399	arg1	hydrogels					401:409	reconstituted collagen hydrogels	378:409	reconstituted collagen hydrogels	378:409	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	5	44	theme	types	1126:1130	arg1	co-existence					1106:1117	the co-existence	1102:1117	the co-existence of two types of fibrils with distinctly different arrangement patterns	1102:1188	TEM discovered the co-existence of two types of fibrils with distinctly different arrangement patterns, explaining the contribution of KGM macromolecules to elasticity generation.					
31819938	7	45	theme	MRI	1416:1418	arg1	T2-mapping					1420:1429	MRI T2-mapping	1416:1429	MRI T2-mapping	1416:1429	Moreover, a quantitative technique of MRI T2-mapping combined with histology is designed to visualize the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels.					
31819938	3	46	theme	hydrogel	592:599	arg1	collagen					572:579	collagen	572:579	collagen	572:579	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	3	46	theme	hydrogel	592:599	arg1	scaffold					601:608	a ternary hydrogel scaffold	582:608	a ternary hydrogel scaffold	582:608	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	9	47	theme	essential	1828:1836	arg1	integration					1838:1848	the essential integration	1824:1848	the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications	1824:2016	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	5	48	theme	different	1159:1167	arg1	patterns					1181:1188	distinctly different arrangement patterns	1148:1188	distinctly different arrangement patterns	1148:1188	TEM discovered the co-existence of two types of fibrils with distinctly different arrangement patterns, explaining the contribution of KGM macromolecules to elasticity generation.					
31819938	3	49	theme	rigid	622:626	arg1	molecules					637:645	rigid collagen molecules	622:645	rigid collagen molecules	622:645	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	9	50	theme	biocompatibility	1858:1873	arg1	integration					1838:1848	the essential integration	1824:1848	the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications	1824:2016	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	2	51	theme	poor	323:326	arg1	properties					339:348	poor mechanical properties	323:348	poor mechanical properties	323:348	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	2	51	theme	poor	323:326	arg1	biodegradation					360:373	rapid biodegradation	354:373	rapid biodegradation	354:373	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	2	51	theme	poor	323:326	arg1	bottlenecks					432:442	the bottlenecks	428:442	the bottlenecks for their further development especially for vascular tissue engineering	428:515	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	9	52	theme	controllable	1876:1887	arg1	biodegradation					1889:1902	controllable biodegradation	1876:1902	controllable biodegradation	1876:1902	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	3	53	theme	flexible	648:655	arg1	chains					682:687	flexible konjac glucomannan (KGM) chains	648:687	flexible konjac glucomannan (KGM) chains	648:687	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	6	54	theme	implants	1304:1311	arg1	variations					1279:1288	The in vivo variations	1267:1288	The in vivo variations of Col/KGM/PA implants	1267:1311	The in vivo variations of Col/KGM/PA implants are visualized in real-time by magnetic resonance imaging (MRI).					
31819938	7	55	dep	in	1484:1485	arg1	vivo					1487:1490	vivo	1487:1490	vivo	1487:1490	Moreover, a quantitative technique of MRI T2-mapping combined with histology is designed to visualize the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels.					
31819938	2	56	theme	rapid	354:358	arg1	properties					339:348	poor mechanical properties	323:348	poor mechanical properties	323:348	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	2	56	theme	rapid	354:358	arg1	biodegradation					360:373	rapid biodegradation	354:373	rapid biodegradation	354:373	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	2	56	theme	rapid	354:358	arg1	bottlenecks					432:442	the bottlenecks	428:442	the bottlenecks for their further development especially for vascular tissue engineering	428:515	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	9	57	theme	mechanical	1917:1926	arg1	properties					1928:1937	improved mechanical properties	1908:1937	improved mechanical properties	1908:1937	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	8	58	theme	respective	1622:1631	arg1	processes					1633:1641	the respective processes	1618:1641	the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants	1618:1726	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	3	59	theme	KGM	677:679	arg1	chains					682:687	flexible konjac glucomannan (KGM) chains	648:687	flexible konjac glucomannan (KGM) chains	648:687	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	4	60	theme	anti-calcification	1040:1057	arg1	activities					1075:1084	anti-calcification and antioxidant activities	1040:1084	anti-calcification and antioxidant activities	1040:1084	PA endows these scaffolds with controllable biodegradation and anti-calcification and antioxidant activities.					
31819938	3	61	theme	Col/KGM/PA	873:882	arg1	hydrogel					884:891	the so-obtained Col/KGM/PA hydrogel	857:891	the so-obtained Col/KGM/PA hydrogel	857:891	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	5	62	theme	macromolecules	1226:1239	arg1	contribution					1206:1217	the contribution	1202:1217	the contribution of KGM macromolecules to elasticity generation	1202:1264	TEM discovered the co-existence of two types of fibrils with distinctly different arrangement patterns, explaining the contribution of KGM macromolecules to elasticity generation.					
31819938	3	63	theme	glucomannan	664:674	arg1	chains					682:687	flexible konjac glucomannan (KGM) chains	648:687	flexible konjac glucomannan (KGM) chains	648:687	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	7	64	theme	erosion	1535:1541	arg1	mechanism					1507:1515	the in vivo biodegradation mechanism	1480:1515	the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels	1480:1561	Moreover, a quantitative technique of MRI T2-mapping combined with histology is designed to visualize the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels.					
31819938	6	65	dep	in	1271:1272	arg1	vivo					1274:1277	vivo	1274:1277	vivo	1274:1277	The in vivo variations of Col/KGM/PA implants are visualized in real-time by magnetic resonance imaging (MRI).					
31819938	3	66	theme	biocompatible	693:705	arg1	crosslinkers					707:718	biocompatible crosslinkers	693:718	biocompatible crosslinkers of proanthocyanidin (PA)	693:743	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	3	67	theme	good	960:963	arg1	elasticity					965:974	good elasticity	960:974	strength but also good elasticity	942:974	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	9	68	theme	scaffold	1964:1971	arg1	material					1973:1980	a desired scaffold material	1954:1980	a desired scaffold material for tissue engineering applications	1954:2016	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	0	69	theme	MRI	106:108	arg1	biodegradation					120:133	MRI trackable biodegradation	106:133	MRI trackable biodegradation	106:133	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	7	70	theme	biodegradation	1492:1505	arg1	mechanism					1507:1515	the in vivo biodegradation mechanism	1480:1515	the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels	1480:1561	Moreover, a quantitative technique of MRI T2-mapping combined with histology is designed to visualize the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels.					
31819938	9	71	theme	engineering	1993:2003	arg1	applications					2005:2016	tissue engineering applications	1986:2016	tissue engineering applications	1986:2016	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	0	72	theme	potential	139:147	arg1	engineering					156:166	potential tissue engineering	139:166	potential tissue engineering	139:166	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	4	73	theme	antioxidant	1063:1073	arg1	activities					1075:1084	anti-calcification and antioxidant activities	1040:1084	anti-calcification and antioxidant activities	1040:1084	PA endows these scaffolds with controllable biodegradation and anti-calcification and antioxidant activities.					
31819938	8	74	theme	controlled	1699:1708	arg1	implants					1719:1726	these controlled hydrogel implants	1693:1726	these controlled hydrogel implants	1693:1726	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	5	75	theme	elasticity	1244:1253	arg1	generation					1255:1264	elasticity generation	1244:1264	elasticity generation	1244:1264	TEM discovered the co-existence of two types of fibrils with distinctly different arrangement patterns, explaining the contribution of KGM macromolecules to elasticity generation.					
31819938	8	76	theme	implants	1719:1726	arg1	dehydration					1678:1688	in vivo dehydration	1670:1688	in vivo dehydration	1670:1688	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	8	76	theme	implants	1719:1726	arg1	degradation					1654:1664	in vivo degradation	1646:1664	in vivo degradation	1646:1664	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	2	77	theme	further	454:460	arg1	development					462:472	their further development	448:472	their further development	448:472	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	0	78	theme	collagen/konjac	29:43	arg1	hydrogel					57:64	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel	0:64	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering	0:166	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	3	79	theme	self-assembly	539:551	arg1	characteristics					553:567	the self-assembly characteristics	535:567	the self-assembly characteristics	535:567	Herein, based on the self-assembly characteristics of collagen, a ternary hydrogel scaffold, comprising rigid collagen molecules, flexible konjac glucomannan (KGM) chains and biocompatible crosslinkers of proanthocyanidin (PA), has been designed to achieve a synergistic interaction for essentially optimizing the mechanical properties of the so-obtained Col/KGM/PA hydrogel, which possesses not only substantially improved strength but also good elasticity.					
31819938	0	80	theme	improved	71:78	arg1	properties					91:100	improved mechanical properties	71:100	improved mechanical properties	71:100	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	8	81	dep	in	1646:1647	arg1	vivo					1649:1652	vivo	1649:1652	vivo	1649:1652	Simultaneously, three different relationships between the respective processes of in vivo degradation and in vivo dehydration of these controlled hydrogel implants were clearly revealed by this technique.					
31819938	0	82	with	hydrogel	57:64	arg1	biodegradation					120:133	MRI trackable biodegradation	106:133	MRI trackable biodegradation	106:133	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	0	82	with	hydrogel	57:64	arg1	properties					91:100	improved mechanical properties	71:100	improved mechanical properties	71:100	Proanthocyanidin-crosslinked collagen/konjac glucomannan hydrogel with improved mechanical properties and MRI trackable biodegradation for potential tissue engineering scaffolds.					
31819938	9	83	theme	Col/KGM/PA	1785:1794	arg1	hydrogel					1806:1813	Such a designed Col/KGM/PA composite hydrogel	1769:1813	Such a designed Col/KGM/PA composite hydrogel	1769:1813	Such a designed Col/KGM/PA composite hydrogel realizes the essential integration of good biocompatibility, controllable biodegradation and improved mechanical properties for developing a desired scaffold material for tissue engineering applications.					
31819938	7	84	theme	T2-mapping	1420:1429	arg1	technique					1403:1411	a quantitative technique	1388:1411	a quantitative technique of MRI T2-mapping combined with histology	1388:1453	Moreover, a quantitative technique of MRI T2-mapping combined with histology is designed to visualize the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels.					
31819938	5	85	theme	arrangement	1169:1179	arg1	patterns					1181:1188	distinctly different arrangement patterns	1148:1188	distinctly different arrangement patterns	1148:1188	TEM discovered the co-existence of two types of fibrils with distinctly different arrangement patterns, explaining the contribution of KGM macromolecules to elasticity generation.					
31819938	2	86	theme	hydrogels	401:409	arg1	properties					339:348	poor mechanical properties	323:348	poor mechanical properties	323:348	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	2	86	theme	hydrogels	401:409	arg1	biodegradation					360:373	rapid biodegradation	354:373	rapid biodegradation	354:373	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	2	86	theme	hydrogels	401:409	arg1	bottlenecks					432:442	the bottlenecks	428:442	the bottlenecks for their further development especially for vascular tissue engineering	428:515	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	5	87	theme	fibrils	1135:1141	arg1	types					1126:1130	two types	1122:1130	two types of fibrils with distinctly different arrangement patterns	1122:1188	TEM discovered the co-existence of two types of fibrils with distinctly different arrangement patterns, explaining the contribution of KGM macromolecules to elasticity generation.					
31819938	6	88	theme	in	1271:1272	arg1	variations					1279:1288	The in vivo variations	1267:1288	The in vivo variations of Col/KGM/PA implants	1267:1311	The in vivo variations of Col/KGM/PA implants are visualized in real-time by magnetic resonance imaging (MRI).					
31819938	2	89	theme	reconstituted	378:390	arg1	hydrogels					401:409	reconstituted collagen hydrogels	378:409	reconstituted collagen hydrogels	378:409	However, the poor mechanical properties and rapid biodegradation of reconstituted collagen hydrogels have always been the bottlenecks for their further development especially for vascular tissue engineering.					
31819938	7	90	theme	quantitative	1390:1401	arg1	technique					1403:1411	a quantitative technique	1388:1411	a quantitative technique of MRI T2-mapping combined with histology	1388:1453	Moreover, a quantitative technique of MRI T2-mapping combined with histology is designed to visualize the in vivo biodegradation mechanism of layer-by-layer erosion for these hydrogels.					
31819938	5	91	with	fibrils	1135:1141	arg1	patterns					1181:1188	distinctly different arrangement patterns	1148:1188	distinctly different arrangement patterns	1148:1188	TEM discovered the co-existence of two types of fibrils with distinctly different arrangement patterns, explaining the contribution of KGM macromolecules to elasticity generation.					
30666109	2	0	theme	silver	442:447	arg1	nanoparticles					449:461	silver nanoparticles	442:461	silver nanoparticles (nAg)	442:467	METHODS PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared.					
30666109	2	0	theme	silver	442:447	arg1	nAg					464:466	nAg	464:466	nAg	464:466	METHODS PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared.					
30666109	4	1	theme	nCS	674:676	arg1	ratios					654:659	Different ratios	644:659	Different ratios of nPLGA and nCS	644:676	Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined.					
30666109	0	2	theme	lactic-co-glycolic	67:84	arg1	poly					62:65	poly	62:65	poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles	62:116	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	0	2	theme	lactic-co-glycolic	67:84	arg1	acid					86:89	lactic-co-glycolic acid	67:89	lactic-co-glycolic acid	67:89	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	5	3	from	nCS	858:860	arg1	ratio					873:877	optimal ratio	865:877	optimal ratio	865:877	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	11	4	theme	50	1658:1659	arg1	µg/mL					1661:1665	µg/mL	1661:1665	µg/mL	1661:1665	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	3	5	theme	cells	619:623	arg1	mineralization					580:593	mineralization	580:593	mineralization	580:593	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	3	5	theme	cells	619:623	arg1	proliferation					562:574	proliferation	562:574	proliferation	562:574	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	0	6	theme	/chitosan/Ag	91:102	arg1	nanoparticles					104:116	poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles	62:116	poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles	62:116	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	7	7	from	nAg	1192:1194	arg1	concentrations					1203:1216	low concentrations	1199:1216	low concentrations	1199:1216	nCS and nAg in low concentrations showed antibacterial activity; however, nAg inhibited cell proliferation.					
30666109	3	8	theme	antibacterial	488:500	arg1	properties					502:511	The antibacterial properties	484:511	The antibacterial properties of single nanoparticles	484:535	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	3	9	from	properties	502:511	arg1	mineralization					580:593	mineralization	580:593	mineralization	580:593	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	3	9	from	properties	502:511	arg1	proliferation					562:574	proliferation	562:574	proliferation	562:574	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	9	10	theme	cell	1513:1516	arg1	mineralization					1518:1531	cell mineralization	1513:1531	cell mineralization	1513:1531	nPLGA/nCS/nAg complex, which had the optimal proportion of the three materials, showed no cytotoxicity and contributed to cell mineralization.					
30666109	5	11	from	nPLGA	848:852	arg1	ratio					873:877	optimal ratio	865:877	optimal ratio	865:877	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	4	12	theme	periodontal	733:743	arg1	cells					754:758	periodontal membrane cells	733:758	periodontal membrane cells	733:758	Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined.					
30666109	11	13	theme	materials	1721:1729	arg1	ratio					1635:1639	The 3:7 ratio	1627:1639	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg	1627:1669	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	11	13	theme	materials	1721:1729	arg1	proportion					1697:1706	the optimal proportion	1685:1706	the optimal proportion of the three materials	1685:1729	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	1	14	theme	materials	331:339	arg1	ratio					310:314	the optimal composite ratio	288:314	the optimal composite ratio of these three materials for periodontal tissue regeneration	288:375	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	4	15	theme	optimal	809:815	arg1	ratio					817:821	the optimal ratio	805:821	the optimal ratio	805:821	Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined.					
30666109	8	16	theme	nCS	1306:1308	arg1	complex					1310:1316	nCS complex	1306:1316	nCS complex	1306:1316	The nPLGA and nCS complex in 3:7 ratio contributed to cell mineralization and had no cytotoxicity.					
30666109	8	17	from	nPLGA	1296:1300	arg1	ratio					1325:1329	3:7 ratio	1321:1329	3:7 ratio	1321:1329	The nPLGA and nCS complex in 3:7 ratio contributed to cell mineralization and had no cytotoxicity.					
30666109	2	18	theme	METHODS	378:384	arg1	nanoparticles					391:403	METHODS PLGA nanoparticles	378:403	METHODS PLGA nanoparticles (nPLGA)	378:411	METHODS PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared.					
30666109	2	18	theme	METHODS	378:384	arg1	nPLGA					406:410	nPLGA	406:410	nPLGA	406:410	METHODS PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared.					
30666109	0	19	theme	nanoparticles	104:116	arg1	mixture					51:57	the mixture	47:57	the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles	47:116	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	11	20	theme	3:7	1631:1633	arg1	ratio					1635:1639	The 3:7 ratio	1627:1639	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg	1627:1669	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	11	20	theme	3:7	1631:1633	arg1	proportion					1697:1706	the optimal proportion	1685:1706	the optimal proportion of the three materials	1685:1729	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	4	21	theme	cells	754:758	arg1	mineralization					715:728	mineralization	715:728	mineralization	715:728	Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined.					
30666109	4	21	theme	cells	754:758	arg1	proliferation					697:709	proliferation	697:709	proliferation	697:709	Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined.					
30666109	5	22	dep	proliferation	966:978	arg1	the					962:964	the	962:964	the	962:964	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	1	23	theme	periodontal	345:355	arg1	regeneration					364:375	periodontal tissue regeneration	345:375	periodontal tissue regeneration	345:375	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	9	24	contain	had	1420:1422	arg1	complex					1405:1411	nPLGA/nCS/nAg complex	1391:1411	nPLGA/nCS/nAg complex	1391:1411	nPLGA/nCS/nAg complex, which had the optimal proportion of the three materials, showed no cytotoxicity and contributed to cell mineralization.					
30666109	9	24	contain	had	1420:1422	arg2	proportion					1436:1445	the optimal proportion	1424:1445	the optimal proportion of the three materials	1424:1468	nPLGA/nCS/nAg complex, which had the optimal proportion of the three materials, showed no cytotoxicity and contributed to cell mineralization.					
30666109	3	25	theme	nanoparticles	523:535	arg1	properties					502:511	The antibacterial properties	484:511	The antibacterial properties of single nanoparticles	484:535	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	3	25	theme	nanoparticles	523:535	arg1	effects					547:553	their effects	541:553	their effects on the proliferation and mineralization of periodontal membrane cells	541:623	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	11	26	theme	nPLGA/nCS	1644:1652	arg1	ratio					1635:1639	The 3:7 ratio	1627:1639	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg	1627:1669	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	11	26	theme	nPLGA/nCS	1644:1652	arg1	proportion					1697:1706	the optimal proportion	1685:1706	the optimal proportion of the three materials	1685:1729	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	11	27	theme	µg/mL	1661:1665	arg1	nAg					1667:1669	50 µg/mL nAg	1658:1669	50 µg/mL nAg	1658:1669	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	2	28	theme	CS	414:415	arg1	nCS					432:434	nCS	432:434	nCS	432:434	METHODS PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared.					
30666109	2	28	theme	CS	414:415	arg1	nanoparticles					417:429	CS nanoparticles	414:429	CS nanoparticles (nCS)	414:435	METHODS PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared.					
30666109	7	29	from	nCS	1184:1186	arg1	concentrations					1203:1216	low concentrations	1199:1216	low concentrations	1199:1216	nCS and nAg in low concentrations showed antibacterial activity; however, nAg inhibited cell proliferation.					
30666109	4	30	theme	Different	644:652	arg1	ratios					654:659	Different ratios	644:659	Different ratios of nPLGA and nCS	644:676	Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined.					
30666109	0	31	theme	tissue	134:139	arg1	engineering					141:151	periodontal tissue engineering	122:151	periodontal tissue engineering	122:151	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	8	32	theme	cell	1346:1349	arg1	mineralization					1351:1364	cell mineralization	1346:1364	cell mineralization	1346:1364	The nPLGA and nCS complex in 3:7 ratio contributed to cell mineralization and had no cytotoxicity.					
30666109	1	33	dep	OBJECTIVE	154:162	arg1	aims					175:178	aims	175:178	aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration	175:375	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	3	34	from	effects	547:553	arg1	mineralization					580:593	mineralization	580:593	mineralization	580:593	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	3	34	from	effects	547:553	arg1	proliferation					562:574	proliferation	562:574	proliferation	562:574	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	1	35	theme	chitosan	208:215	arg1	nanoparticles					191:203	nanoparticles	191:203	nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver	191:270	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	0	36	theme	biological	16:25	arg1	characterization					27:42	biological characterization	16:42	biological characterization	16:42	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	0	37	theme	periodontal	122:132	arg1	engineering					141:151	periodontal tissue engineering	122:151	periodontal tissue engineering	122:151	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	1	38	theme	tissue	357:362	arg1	regeneration					364:375	periodontal tissue regeneration	345:375	periodontal tissue regeneration	345:375	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	3	39	theme	single	516:521	arg1	nanoparticles					523:535	single nanoparticles	516:535	single nanoparticles	516:535	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	9	40	theme	materials	1460:1468	arg1	proportion					1436:1445	the optimal proportion	1424:1445	the optimal proportion of the three materials	1424:1468	nPLGA/nCS/nAg complex, which had the optimal proportion of the three materials, showed no cytotoxicity and contributed to cell mineralization.					
30666109	1	41	theme	silver	265:270	arg1	nanoparticles					191:203	nanoparticles	191:203	nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver	191:270	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	5	42	from	effects	911:917	arg1	mineralization					984:997	mineralization	984:997	mineralization	984:997	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	5	42	from	effects	911:917	arg1	proliferation					966:978	proliferation	966:978	proliferation	966:978	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	5	43	theme	cells	1023:1027	arg1	mineralization					984:997	mineralization	984:997	mineralization	984:997	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	5	43	theme	cells	1023:1027	arg1	proliferation					966:978	proliferation	966:978	proliferation	966:978	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	5	44	theme	materials	949:957	arg1	complex					926:932	the complex	922:932	the complex of these three materials	922:957	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	7	45	theme	cell	1272:1275	arg1	proliferation					1277:1289	cell proliferation	1272:1289	cell proliferation	1272:1289	nCS and nAg in low concentrations showed antibacterial activity; however, nAg inhibited cell proliferation.					
30666109	8	46	dep	nPLGA	1296:1300	arg1	The					1292:1294	The	1292:1294	The	1292:1294	The nPLGA and nCS complex in 3:7 ratio contributed to cell mineralization and had no cytotoxicity.					
30666109	10	47	theme	nPLGA/nCS/nAg	1545:1557	arg1	complex					1559:1565	CONCLUSION nPLGA/nCS/nAg complex	1534:1565	CONCLUSION nPLGA/nCS/nAg complex	1534:1565	CONCLUSION nPLGA/nCS/nAg complex had no cytotoxicity and contributed to cell mineralization.					
30666109	3	48	theme	periodontal	598:608	arg1	cells					619:623	periodontal membrane cells	598:623	periodontal membrane cells	598:623	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	8	49	from	complex	1310:1316	arg1	ratio					1325:1329	3:7 ratio	1321:1329	3:7 ratio	1321:1329	The nPLGA and nCS complex in 3:7 ratio contributed to cell mineralization and had no cytotoxicity.					
30666109	2	50	theme	PLGA	386:389	arg1	nanoparticles					391:403	METHODS PLGA nanoparticles	378:403	METHODS PLGA nanoparticles (nPLGA)	378:411	METHODS PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared.					
30666109	2	50	theme	PLGA	386:389	arg1	nPLGA					406:410	nPLGA	406:410	nPLGA	406:410	METHODS PLGA nanoparticles (nPLGA), CS nanoparticles (nCS), and silver nanoparticles (nAg) were prepared.					
30666109	5	51	theme	membrane	1014:1021	arg1	cells					1023:1027	periodontal membrane cells	1002:1027	periodontal membrane cells	1002:1027	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	10	52	theme	cell	1606:1609	arg1	mineralization					1611:1624	cell mineralization	1606:1624	cell mineralization	1606:1624	CONCLUSION nPLGA/nCS/nAg complex had no cytotoxicity and contributed to cell mineralization.					
30666109	10	53	theme	CONCLUSION	1534:1543	arg1	complex					1559:1565	CONCLUSION nPLGA/nCS/nAg complex	1534:1565	CONCLUSION nPLGA/nCS/nAg complex	1534:1565	CONCLUSION nPLGA/nCS/nAg complex had no cytotoxicity and contributed to cell mineralization.					
30666109	3	54	theme	membrane	610:617	arg1	cells					619:623	periodontal membrane cells	598:623	periodontal membrane cells	598:623	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	10	55	contain	had	1567:1569	arg1	complex					1559:1565	CONCLUSION nPLGA/nCS/nAg complex	1534:1565	CONCLUSION nPLGA/nCS/nAg complex	1534:1565	CONCLUSION nPLGA/nCS/nAg complex had no cytotoxicity and contributed to cell mineralization.					
30666109	10	55	contain	had	1567:1569	arg2	cytotoxicity					1574:1585	no cytotoxicity	1571:1585	no cytotoxicity	1571:1585	CONCLUSION nPLGA/nCS/nAg complex had no cytotoxicity and contributed to cell mineralization.					
30666109	5	56	theme	optimal	865:871	arg1	ratio					873:877	optimal ratio	865:877	optimal ratio	865:877	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	0	57	theme	mixture	51:57	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	0	57	theme	mixture	51:57	arg1	characterization					27:42	biological characterization	16:42	biological characterization	16:42	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	6	58	theme	cell	1163:1166	arg1	mineralization					1168:1181	cell mineralization	1163:1181	cell mineralization	1163:1181	RESULTS The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization.					
30666109	7	59	theme	antibacterial	1225:1237	arg1	activity					1239:1246	antibacterial activity	1225:1246	antibacterial activity	1225:1246	nCS and nAg in low concentrations showed antibacterial activity; however, nAg inhibited cell proliferation.					
30666109	1	60	theme	optimal	292:298	arg1	ratio					310:314	the optimal composite ratio	288:314	the optimal composite ratio of these three materials for periodontal tissue regeneration	288:375	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	11	61	theme	nAg	1667:1669	arg1	ratio					1635:1639	The 3:7 ratio	1627:1639	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg	1627:1669	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	11	61	theme	nAg	1667:1669	arg1	proportion					1697:1706	the optimal proportion	1685:1706	the optimal proportion of the three materials	1685:1729	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	6	62	dep	RESULTS	1070:1076	arg1	found					1108:1112	found	1108:1112	were found to have no cytotoxicity	1103:1136	RESULTS The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization.					
30666109	6	62	dep	RESULTS	1070:1076	arg1	nanoparticles					1089:1101	The single nanoparticles	1078:1101	The single nanoparticles	1078:1101	RESULTS The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization.					
30666109	6	62	dep	RESULTS	1070:1076	arg1	able					1147:1150	able	1147:1150	able	1147:1150	RESULTS The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization.					
30666109	5	63	theme	complex	926:932	arg1	effects					911:917	the effects	907:917	the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells	907:1027	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	6	64	contain	have	1117:1120	arg1	nanoparticles					1089:1101	The single nanoparticles	1078:1101	The single nanoparticles	1078:1101	RESULTS The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization.					
30666109	6	64	contain	have	1117:1120	arg2	cytotoxicity					1125:1136	no cytotoxicity	1122:1136	no cytotoxicity	1122:1136	RESULTS The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization.					
30666109	6	64	contain	have	1117:1120	arg1	able					1147:1150	able	1147:1150	able	1147:1150	RESULTS The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization.					
30666109	4	65	theme	membrane	745:752	arg1	cells					754:758	periodontal membrane cells	733:758	periodontal membrane cells	733:758	Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined.					
30666109	4	66	dep	proliferation	697:709	arg1	the					693:695	the	693:695	the	693:695	Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined.					
30666109	6	67	theme	single	1082:1087	arg1	nanoparticles					1089:1101	The single nanoparticles	1078:1101	The single nanoparticles	1078:1101	RESULTS The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization.					
30666109	6	67	theme	single	1082:1087	arg1	able					1147:1150	able	1147:1150	able	1147:1150	RESULTS The single nanoparticles were found to have no cytotoxicity and were able to promote cell mineralization.					
30666109	1	68	theme	poly	223:226	arg1	nanoparticles					191:203	nanoparticles	191:203	nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver	191:270	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	9	69	theme	optimal	1428:1434	arg1	proportion					1436:1445	the optimal proportion	1424:1445	the optimal proportion of the three materials	1424:1468	nPLGA/nCS/nAg complex, which had the optimal proportion of the three materials, showed no cytotoxicity and contributed to cell mineralization.					
30666109	5	70	theme	periodontal	1002:1012	arg1	cells					1023:1027	periodontal membrane cells	1002:1027	periodontal membrane cells	1002:1027	Finally, nPLGA and nCS in optimal ratio were combined with nAg, and the effects of the complex of these three materials on the proliferation and mineralization of periodontal membrane cells were investigated and tested in animals.					
30666109	3	71	dep	proliferation	562:574	arg1	the					558:560	the	558:560	the	558:560	The antibacterial properties of single nanoparticles and their effects on the proliferation and mineralization of periodontal membrane cells were investigated.					
30666109	4	72	theme	nPLGA	664:668	arg1	ratios					654:659	Different ratios	644:659	Different ratios of nPLGA and nCS	644:676	Different ratios of nPLGA and nCS were combined, the proliferation and mineralization of periodontal membrane cells were investigated, and based on the results, the optimal ratio was determined.					
30666109	9	73	theme	nPLGA/nCS/nAg	1391:1403	arg1	complex					1405:1411	nPLGA/nCS/nAg complex	1391:1411	nPLGA/nCS/nAg complex	1391:1411	nPLGA/nCS/nAg complex, which had the optimal proportion of the three materials, showed no cytotoxicity and contributed to cell mineralization.					
30666109	11	74	theme	optimal	1689:1695	arg1	ratio					1635:1639	The 3:7 ratio	1627:1639	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg	1627:1669	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	11	74	theme	optimal	1689:1695	arg1	proportion					1697:1706	the optimal proportion	1685:1706	the optimal proportion of the three materials	1685:1729	The 3:7 ratio of nPLGA/nCS and 50 µg/mL nAg were found as the optimal proportion of the three materials.					
30666109	0	75	theme	poly	62:65	arg1	nanoparticles					104:116	poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles	62:116	poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles	62:116	Preparation and biological characterization of the mixture of poly(lactic-co-glycolic acid)/chitosan/Ag nanoparticles for periodontal tissue engineering.					
30666109	1	76	theme	composite	300:308	arg1	ratio					310:314	the optimal composite ratio	288:314	the optimal composite ratio of these three materials for periodontal tissue regeneration	288:375	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	8	77	contain	had	1370:1372	arg2	cytotoxicity					1377:1388	no cytotoxicity	1374:1388	no cytotoxicity	1374:1388	The nPLGA and nCS complex in 3:7 ratio contributed to cell mineralization and had no cytotoxicity.					
30666109	8	77	contain	had	1370:1372	arg1	nPLGA					1296:1300	nPLGA	1296:1300	nPLGA	1296:1300	The nPLGA and nCS complex in 3:7 ratio contributed to cell mineralization and had no cytotoxicity.					
30666109	7	78	theme	low	1199:1201	arg1	concentrations					1203:1216	low concentrations	1199:1216	low concentrations	1199:1216	nCS and nAg in low concentrations showed antibacterial activity; however, nAg inhibited cell proliferation.					
30666109	1	79	theme	lactic-co-glycolic	228:245	arg1	poly					223:226	poly	223:226	poly(lactic-co-glycolic acid) (PLGA)	223:258	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30666109	1	79	theme	lactic-co-glycolic	228:245	arg1	acid					247:250	lactic-co-glycolic acid	228:250	lactic-co-glycolic acid	228:250	OBJECTIVE This study aims to produce nanoparticles of chitosan (CS), poly(lactic-co-glycolic acid) (PLGA), and silver and investigate the optimal composite ratio of these three materials for periodontal tissue regeneration.					
30678156	9	0	theme	immunosuppressive	1065:1081	arg1	potential					1083:1091	the immunosuppressive potential	1061:1091	the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6	1061:1143	Gastrointestinal metabolism of PYC revealed the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6 when compared to the control fraction containing microbiota and enzymes only.					
30678156	6	1	theme	partial	822:828	arg1	agonist					830:836	a partial agonist	820:836	a partial agonist of TLR5	820:844	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	6	1	theme	partial	822:828	arg1	PYC					752:754	intact non-metabolized PYC	729:754	intact non-metabolized PYC	729:754	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	1	2	used	used	152:155	arg2	Pycnogenol®					82:92	BACKGROUND Pycnogenol®	71:92	BACKGROUND Pycnogenol® (PYC)	71:98	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	1	2	used	used	152:155	arg2	extract					104:110	an extract	101:110	an extract of French maritime pine bark	101:139	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	1	2	used	used	152:155	arg2	supplement					170:179	a dietary supplement	160:179	a dietary supplement	160:179	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	1	2	used	used	152:155	arg2	PYC					95:97	PYC	95:97	PYC	95:97	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	4	3	theme	membrane	538:545	arg1	TLR5					561:564	TLR5	561:564	TLR5	561:564	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	3	theme	membrane	538:545	arg1	TLR2/6					571:576	TLR2/6	571:576	TLR2/6	571:576	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	3	theme	membrane	538:545	arg1	TLR1/2					553:558	TLR1/2	553:558	TLR1/2	553:558	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	3	theme	membrane	538:545	arg1	TLRs					547:550	cell membrane TLRs	533:550	cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4	533:593	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	6	4	theme	intact	729:734	arg1	agonist					830:836	a partial agonist	820:836	a partial agonist of TLR5	820:844	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	6	4	theme	intact	729:734	arg1	agonist					784:790	an agonist	781:790	an agonist of TLR1/2 and TLR2/6	781:811	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	6	4	theme	intact	729:734	arg1	PYC					752:754	intact non-metabolized PYC	729:754	intact non-metabolized PYC	729:754	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	8	5	with	complexes	931:939	arg1	LPS					967:969	LPS	967:969	LPS	967:969	However, after the formation of complexes with lipopolysaccharides (LPS), it is a potent activator of TLR4 signaling.					
30678156	8	5	with	complexes	931:939	arg1	lipopolysaccharides					946:964	lipopolysaccharides	946:964	lipopolysaccharides (LPS)	946:970	However, after the formation of complexes with lipopolysaccharides (LPS), it is a potent activator of TLR4 signaling.					
30678156	4	6	theme	other	579:583	arg1	TLR5					561:564	TLR5	561:564	TLR5	561:564	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	6	theme	other	579:583	arg1	TLR2/6					571:576	TLR2/6	571:576	TLR2/6	571:576	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	6	theme	other	579:583	arg1	TLR1/2					553:558	TLR1/2	553:558	TLR1/2	553:558	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	6	theme	other	579:583	arg1	TLRs					547:550	cell membrane TLRs	533:550	cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4	533:593	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	9	7	theme	retentate	1100:1108	arg1	fraction					1110:1117	the retentate fraction	1096:1117	the retentate fraction	1096:1117	Gastrointestinal metabolism of PYC revealed the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6 when compared to the control fraction containing microbiota and enzymes only.					
30678156	2	8	theme	Toll-like	255:263	arg1	receptor					265:272	the Toll-like receptor 4	251:274	the Toll-like receptor 4 (TLR4) pathway	251:289	PYC has been shown to exert anti-inflammatory actions via inhibiting the Toll-like receptor 4 (TLR4) pathway.					
30678156	2	8	theme	Toll-like	255:263	arg1	TLR4					277:280	TLR4	277:280	TLR4	277:280	PYC has been shown to exert anti-inflammatory actions via inhibiting the Toll-like receptor 4 (TLR4) pathway.					
30678156	6	9	theme	TLR2/6	806:811	arg1	agonist					784:790	an agonist	781:790	an agonist of TLR1/2 and TLR2/6	781:811	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	6	9	theme	TLR2/6	806:811	arg1	PYC					752:754	intact non-metabolized PYC	729:754	intact non-metabolized PYC	729:754	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	12	10	theme	potential	1571:1579	arg1	inhibitor					1581:1589	a potential inhibitor	1569:1589	a potential inhibitor of TLRs signaling	1569:1607	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	12	10	theme	potential	1571:1579	arg1	activity					1557:1564	its biological activity	1542:1564	its biological activity	1542:1564	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	13	11	theme	TLR	1740:1742	arg1	agonists					1744:1751	the microbiota-derived TLR agonists	1717:1751	the microbiota-derived TLR agonists (retentate fraction)	1717:1772	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	11	theme	TLR	1740:1742	arg1	fraction					1764:1771	retentate fraction	1754:1771	retentate fraction	1754:1771	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	5	12	theme	metabolism	637:646	arg1	effect					610:615	the effect	606:615	the effect of gastrointestinal metabolism on the immunomodulatory effects of PYC	606:685	Moreover, the effect of gastrointestinal metabolism on the immunomodulatory effects of PYC was investigated.					
30678156	12	13	theme	biological	1546:1555	arg1	inhibitor					1581:1589	a potential inhibitor	1569:1589	a potential inhibitor of TLRs signaling	1569:1607	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	12	13	theme	biological	1546:1555	arg1	activity					1557:1564	its biological activity	1542:1564	its biological activity	1542:1564	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	1	14	theme	maritime	122:129	arg1	bark					136:139	French maritime pine bark	115:139	French maritime pine bark	115:139	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	10	15	theme	IL-10	1322:1326	arg1	secretion					1328:1336	anti-inflammatory IL-10 secretion	1304:1336	anti-inflammatory IL-10 secretion	1304:1336	The dialyzed fraction containing PYC metabolites revealed the capacity to induce anti-inflammatory IL-10 secretion.					
30678156	13	16	theme	dialysate	1884:1892	arg1	macrophages					1871:1881	THP-1 macrophages	1865:1881	THP-1 macrophages (dialysate fraction)	1865:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	16	theme	dialysate	1884:1892	arg1	fraction					1894:1901	dialysate fraction	1884:1901	dialysate fraction	1884:1901	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	9	17	theme	control	1166:1172	arg1	fraction					1174:1181	the control fraction	1162:1181	the control fraction containing microbiota and enzymes only	1162:1220	Gastrointestinal metabolism of PYC revealed the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6 when compared to the control fraction containing microbiota and enzymes only.					
30678156	1	18	theme	bark	136:139	arg1	Pycnogenol®					82:92	BACKGROUND Pycnogenol®	71:92	BACKGROUND Pycnogenol® (PYC)	71:98	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	1	18	theme	bark	136:139	arg1	extract					104:110	an extract	101:110	an extract of French maritime pine bark	101:139	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	13	19	contain	possesses	1786:1794	arg2	PYC					1647:1649	metabolized PYC	1635:1649	metabolized PYC	1635:1649	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	19	contain	possesses	1786:1794	arg2	potential					1814:1822	anti-inflammatory potential	1796:1822	anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction)	1796:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	19	contain	possesses	1786:1794	arg2	agonist					1669:1675	a partial agonist	1659:1675	a partial agonist of TLR1/2 and TLR2/6	1659:1696	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	19	contain	possesses	1786:1794	arg1	it					1783:1784	it	1783:1784	it	1783:1784	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	5	20	theme	PYC	683:685	arg1	effects					672:678	the immunomodulatory effects	651:678	the immunomodulatory effects of PYC	651:685	Moreover, the effect of gastrointestinal metabolism on the immunomodulatory effects of PYC was investigated.					
30678156	3	21	theme	other	317:321	arg1	receptors					323:331	the other receptors	313:331	the other receptors from the TLR family	313:351	However, the role of the other receptors from the TLR family in the immunomodulatory activity of PYC has not been described so far.					
30678156	4	22	theme	study	444:448	arg1	aim					432:434	The aim	428:434	The aim of this study	428:448	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	13	23	theme	TLR1/2	1680:1685	arg1	potential					1814:1822	anti-inflammatory potential	1796:1822	anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction)	1796:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	23	theme	TLR1/2	1680:1685	arg1	agonist					1669:1675	a partial agonist	1659:1675	a partial agonist of TLR1/2 and TLR2/6	1659:1696	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	23	theme	TLR1/2	1680:1685	arg1	PYC					1647:1649	metabolized PYC	1635:1649	metabolized PYC	1635:1649	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	10	24	theme	anti-inflammatory	1304:1320	arg1	secretion					1328:1336	anti-inflammatory IL-10 secretion	1304:1336	anti-inflammatory IL-10 secretion	1304:1336	The dialyzed fraction containing PYC metabolites revealed the capacity to induce anti-inflammatory IL-10 secretion.					
30678156	12	25	dep	CONCLUSIONS	1464:1474	arg1	showed					1487:1492	showed	1487:1492	showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling	1487:1607	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	8	26	theme	complexes	931:939	arg1	formation					918:926	the formation	914:926	the formation of complexes with lipopolysaccharides (LPS)	914:970	However, after the formation of complexes with lipopolysaccharides (LPS), it is a potent activator of TLR4 signaling.					
30678156	13	27	from	macrophages	1871:1881	arg1	induction					1841:1849	the induction	1837:1849	the induction of IL-10 from THP-1 macrophages (dialysate fraction)	1837:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	12	28	theme	gastrointestinal	1499:1514	arg1	metabolism					1516:1525	gastrointestinal metabolism	1499:1525	gastrointestinal metabolism of PYC	1499:1532	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	3	29	theme	TLR	342:344	arg1	family					346:351	the TLR family	338:351	the TLR family	338:351	However, the role of the other receptors from the TLR family in the immunomodulatory activity of PYC has not been described so far.					
30678156	9	30	contain	containing	1183:1192	arg2	enzymes					1209:1215	enzymes	1209:1215	enzymes	1209:1215	Gastrointestinal metabolism of PYC revealed the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6 when compared to the control fraction containing microbiota and enzymes only.					
30678156	9	30	contain	containing	1183:1192	arg2	microbiota					1194:1203	microbiota	1194:1203	microbiota	1194:1203	Gastrointestinal metabolism of PYC revealed the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6 when compared to the control fraction containing microbiota and enzymes only.					
30678156	9	30	contain	containing	1183:1192	arg1	fraction					1174:1181	the control fraction	1162:1181	the control fraction containing microbiota and enzymes only	1162:1220	Gastrointestinal metabolism of PYC revealed the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6 when compared to the control fraction containing microbiota and enzymes only.					
30678156	0	31	theme	Toll-Like	0:8	arg1	Activity					46:53	Toll-Like Receptor-Dependent Immunomodulatory Activity	0:53	Toll-Like Receptor-Dependent Immunomodulatory Activity of Pycnogenol®	0:68	Toll-Like Receptor-Dependent Immunomodulatory Activity of Pycnogenol®.					
30678156	3	32	from	role	305:308	arg1	family					346:351	the TLR family	338:351	the TLR family	338:351	However, the role of the other receptors from the TLR family in the immunomodulatory activity of PYC has not been described so far.					
30678156	3	32	from	role	305:308	arg1	activity					377:384	the immunomodulatory activity	356:384	the immunomodulatory activity of PYC	356:391	However, the role of the other receptors from the TLR family in the immunomodulatory activity of PYC has not been described so far.					
30678156	13	33	theme	anti-inflammatory	1796:1812	arg1	potential					1814:1822	anti-inflammatory potential	1796:1822	anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction)	1796:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	33	theme	anti-inflammatory	1796:1812	arg1	agonist					1669:1675	a partial agonist	1659:1675	a partial agonist of TLR1/2 and TLR2/6	1659:1696	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	33	theme	anti-inflammatory	1796:1812	arg1	PYC					1647:1649	metabolized PYC	1635:1649	metabolized PYC	1635:1649	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	0	34	theme	Immunomodulatory	29:44	arg1	Activity					46:53	Toll-Like Receptor-Dependent Immunomodulatory Activity	0:53	Toll-Like Receptor-Dependent Immunomodulatory Activity of Pycnogenol®	0:68	Toll-Like Receptor-Dependent Immunomodulatory Activity of Pycnogenol®.					
30678156	3	35	theme	immunomodulatory	360:375	arg1	activity					377:384	the immunomodulatory activity	356:384	the immunomodulatory activity of PYC	356:391	However, the role of the other receptors from the TLR family in the immunomodulatory activity of PYC has not been described so far.					
30678156	13	36	theme	metabolized	1635:1645	arg1	potential					1814:1822	anti-inflammatory potential	1796:1822	anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction)	1796:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	36	theme	metabolized	1635:1645	arg1	agonist					1669:1675	a partial agonist	1659:1675	a partial agonist of TLR1/2 and TLR2/6	1659:1696	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	36	theme	metabolized	1635:1645	arg1	PYC					1647:1649	metabolized PYC	1635:1649	metabolized PYC	1635:1649	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	11	37	theme	microbiota	1397:1406	arg1	composition					1408:1418	the colonic microbiota composition	1385:1418	the colonic microbiota composition	1385:1418	Finally, microbially metabolized PYC affected the colonic microbiota composition during in vitro gastrointestinal digestion.					
30678156	4	38	dep	was	450:452	arg1	AIM					424:426	AIM	424:426	AIM	424:426	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	10	39	theme	PYC	1256:1258	arg1	metabolites					1260:1270	PYC metabolites	1256:1270	PYC metabolites	1256:1270	The dialyzed fraction containing PYC metabolites revealed the capacity to induce anti-inflammatory IL-10 secretion.					
30678156	8	40	theme	potent	981:986	arg1	activator					988:996	a potent activator	979:996	a potent activator of TLR4 signaling	979:1014	However, after the formation of complexes with lipopolysaccharides (LPS), it is a potent activator of TLR4 signaling.					
30678156	8	40	theme	potent	981:986	arg1	it					973:974	it	973:974	it	973:974	However, after the formation of complexes with lipopolysaccharides (LPS), it is a potent activator of TLR4 signaling.					
30678156	9	41	theme	PYC	1048:1050	arg1	metabolism					1034:1043	Gastrointestinal metabolism	1017:1043	Gastrointestinal metabolism of PYC	1017:1050	Gastrointestinal metabolism of PYC revealed the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6 when compared to the control fraction containing microbiota and enzymes only.					
30678156	8	42	theme	signaling	1006:1014	arg1	activator					988:996	a potent activator	979:996	a potent activator of TLR4 signaling	979:1014	However, after the formation of complexes with lipopolysaccharides (LPS), it is a potent activator of TLR4 signaling.					
30678156	8	42	theme	signaling	1006:1014	arg1	it					973:974	it	973:974	it	973:974	However, after the formation of complexes with lipopolysaccharides (LPS), it is a potent activator of TLR4 signaling.					
30678156	6	43	theme	TLR5	841:844	arg1	agonist					830:836	a partial agonist	820:836	a partial agonist of TLR5	820:844	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	6	43	theme	TLR5	841:844	arg1	PYC					752:754	intact non-metabolized PYC	729:754	intact non-metabolized PYC	729:754	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	1	44	theme	BACKGROUND	71:80	arg1	PYC					95:97	PYC	95:97	PYC	95:97	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	1	44	theme	BACKGROUND	71:80	arg1	Pycnogenol®					82:92	BACKGROUND Pycnogenol®	71:92	BACKGROUND Pycnogenol® (PYC)	71:98	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	1	44	theme	BACKGROUND	71:80	arg1	supplement					170:179	a dietary supplement	160:179	a dietary supplement	160:179	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	1	44	theme	BACKGROUND	71:80	arg1	extract					104:110	an extract	101:110	an extract of French maritime pine bark	101:139	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	13	45	theme	agonists	1744:1751	arg1	presence					1705:1712	the presence	1701:1712	the presence of the microbiota-derived TLR agonists (retentate fraction)	1701:1772	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	6	46	theme	non-metabolized	736:750	arg1	agonist					830:836	a partial agonist	820:836	a partial agonist of TLR5	820:844	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	6	46	theme	non-metabolized	736:750	arg1	agonist					784:790	an agonist	781:790	an agonist of TLR1/2 and TLR2/6	781:811	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	6	46	theme	non-metabolized	736:750	arg1	PYC					752:754	intact non-metabolized PYC	729:754	intact non-metabolized PYC	729:754	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	5	47	from	effect	610:615	arg1	effects					672:678	the immunomodulatory effects	651:678	the immunomodulatory effects of PYC	651:685	Moreover, the effect of gastrointestinal metabolism on the immunomodulatory effects of PYC was investigated.					
30678156	6	48	dep	FINDINGS	705:712	arg1	We					714:715	We	714:715	We	714:715	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	4	49	theme	cell	533:536	arg1	TLR5					561:564	TLR5	561:564	TLR5	561:564	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	49	theme	cell	533:536	arg1	TLR2/6					571:576	TLR2/6	571:576	TLR2/6	571:576	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	49	theme	cell	533:536	arg1	TLR1/2					553:558	TLR1/2	553:558	TLR1/2	553:558	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	49	theme	cell	533:536	arg1	TLRs					547:550	cell membrane TLRs	533:550	cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4	533:593	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	13	50	theme	retentate	1754:1762	arg1	agonists					1744:1751	the microbiota-derived TLR agonists	1717:1751	the microbiota-derived TLR agonists (retentate fraction)	1717:1772	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	50	theme	retentate	1754:1762	arg1	fraction					1764:1771	retentate fraction	1754:1771	retentate fraction	1754:1771	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	2	51	theme	receptor	265:272	arg1	pathway					283:289	the Toll-like receptor 4 (TLR4) pathway	251:289	the Toll-like receptor 4 (TLR4) pathway	251:289	PYC has been shown to exert anti-inflammatory actions via inhibiting the Toll-like receptor 4 (TLR4) pathway.					
30678156	9	52	theme	fraction	1110:1117	arg1	potential					1083:1091	the immunosuppressive potential	1061:1091	the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6	1061:1143	Gastrointestinal metabolism of PYC revealed the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6 when compared to the control fraction containing microbiota and enzymes only.					
30678156	1	53	theme	dietary	162:168	arg1	Pycnogenol®					82:92	BACKGROUND Pycnogenol®	71:92	BACKGROUND Pycnogenol® (PYC)	71:98	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	1	53	theme	dietary	162:168	arg1	supplement					170:179	a dietary supplement	160:179	a dietary supplement	160:179	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	13	54	theme	microbiota-derived	1721:1738	arg1	agonists					1744:1751	the microbiota-derived TLR agonists	1717:1751	the microbiota-derived TLR agonists (retentate fraction)	1717:1772	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	54	theme	microbiota-derived	1721:1738	arg1	fraction					1764:1771	retentate fraction	1754:1771	retentate fraction	1754:1771	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	12	55	theme	TLRs	1594:1597	arg1	signaling					1599:1607	TLRs signaling	1594:1607	TLRs signaling	1594:1607	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	13	56	theme	TLR2/6	1691:1696	arg1	potential					1814:1822	anti-inflammatory potential	1796:1822	anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction)	1796:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	56	theme	TLR2/6	1691:1696	arg1	agonist					1669:1675	a partial agonist	1659:1675	a partial agonist of TLR1/2 and TLR2/6	1659:1696	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	56	theme	TLR2/6	1691:1696	arg1	PYC					1647:1649	metabolized PYC	1635:1649	metabolized PYC	1635:1649	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	5	57	theme	gastrointestinal	620:635	arg1	metabolism					637:646	gastrointestinal metabolism	620:646	gastrointestinal metabolism	620:646	Moreover, the effect of gastrointestinal metabolism on the immunomodulatory effects of PYC was investigated.					
30678156	2	58	theme	anti-inflammatory	210:226	arg1	actions					228:234	anti-inflammatory actions	210:234	anti-inflammatory actions	210:234	PYC has been shown to exert anti-inflammatory actions via inhibiting the Toll-like receptor 4 (TLR4) pathway.					
30678156	1	59	theme	French	115:120	arg1	bark					136:139	French maritime pine bark	115:139	French maritime pine bark	115:139	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	7	60	from	PYC	847:849	arg1	own					858:860	own	858:860	own	858:860	PYC on its own does not agonize or antagonize TLR4.					
30678156	11	61	theme	gastrointestinal	1436:1451	arg1	digestion					1453:1461	in vitro gastrointestinal digestion	1427:1461	in vitro gastrointestinal digestion	1427:1461	Finally, microbially metabolized PYC affected the colonic microbiota composition during in vitro gastrointestinal digestion.					
30678156	1	62	theme	pine	131:134	arg1	bark					136:139	French maritime pine bark	115:139	French maritime pine bark	115:139	BACKGROUND Pycnogenol® (PYC), an extract of French maritime pine bark, is widely used as a dietary supplement.					
30678156	12	63	theme	PYC	1530:1532	arg1	metabolism					1516:1525	gastrointestinal metabolism	1499:1525	gastrointestinal metabolism of PYC	1499:1532	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	11	64	theme	in	1427:1428	arg1	digestion					1453:1461	in vitro gastrointestinal digestion	1427:1461	in vitro gastrointestinal digestion	1427:1461	Finally, microbially metabolized PYC affected the colonic microbiota composition during in vitro gastrointestinal digestion.					
30678156	4	65	dep	TLRs	547:550	arg1	TLR5					561:564	TLR5	561:564	TLR5	561:564	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	65	dep	TLRs	547:550	arg1	TLR2/6					571:576	TLR2/6	571:576	TLR2/6	571:576	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	65	dep	TLRs	547:550	arg1	TLR1/2					553:558	TLR1/2	553:558	TLR1/2	553:558	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	4	65	dep	TLRs	547:550	arg1	TLRs					547:550	cell membrane TLRs	533:550	cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4	533:593	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	13	66	theme	IL-10	1854:1858	arg1	induction					1841:1849	the induction	1837:1849	the induction of IL-10 from THP-1 macrophages (dialysate fraction)	1837:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	11	67	dep	in	1427:1428	arg1	vitro					1430:1434	vitro	1430:1434	vitro	1430:1434	Finally, microbially metabolized PYC affected the colonic microbiota composition during in vitro gastrointestinal digestion.					
30678156	13	68	theme	THP-1	1865:1869	arg1	macrophages					1871:1881	THP-1 macrophages	1865:1881	THP-1 macrophages (dialysate fraction)	1865:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	68	theme	THP-1	1865:1869	arg1	fraction					1894:1901	dialysate fraction	1884:1901	dialysate fraction	1884:1901	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	3	69	theme	receptors	323:331	arg1	role					305:308	the role	301:308	the role of the other receptors from the TLR family in the immunomodulatory activity of PYC	301:391	However, the role of the other receptors from the TLR family in the immunomodulatory activity of PYC has not been described so far.					
30678156	5	70	theme	immunomodulatory	655:670	arg1	effects					672:678	the immunomodulatory effects	651:678	the immunomodulatory effects of PYC	651:685	Moreover, the effect of gastrointestinal metabolism on the immunomodulatory effects of PYC was investigated.					
30678156	11	71	theme	metabolized	1360:1370	arg1	PYC					1372:1374	microbially metabolized PYC	1348:1374	microbially metabolized PYC	1348:1374	Finally, microbially metabolized PYC affected the colonic microbiota composition during in vitro gastrointestinal digestion.					
30678156	0	72	theme	Receptor-Dependent	10:27	arg1	Activity					46:53	Toll-Like Receptor-Dependent Immunomodulatory Activity	0:53	Toll-Like Receptor-Dependent Immunomodulatory Activity of Pycnogenol®	0:68	Toll-Like Receptor-Dependent Immunomodulatory Activity of Pycnogenol®.					
30678156	3	73	from	family	346:351	arg1	role					305:308	the role	301:308	the role of the other receptors from the TLR family in the immunomodulatory activity of PYC	301:391	However, the role of the other receptors from the TLR family in the immunomodulatory activity of PYC has not been described so far.					
30678156	3	73	from	family	346:351	arg1	receptors					323:331	the other receptors	313:331	the other receptors from the TLR family	313:351	However, the role of the other receptors from the TLR family in the immunomodulatory activity of PYC has not been described so far.					
30678156	13	74	theme	partial	1661:1667	arg1	potential					1814:1822	anti-inflammatory potential	1796:1822	anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction)	1796:1902	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	74	theme	partial	1661:1667	arg1	agonist					1669:1675	a partial agonist	1659:1675	a partial agonist of TLR1/2 and TLR2/6	1659:1696	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	74	theme	partial	1661:1667	arg1	PYC					1647:1649	metabolized PYC	1635:1649	metabolized PYC	1635:1649	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	10	75	theme	dialyzed	1227:1234	arg1	fraction					1236:1243	The dialyzed fraction	1223:1243	The dialyzed fraction containing PYC metabolites	1223:1270	The dialyzed fraction containing PYC metabolites revealed the capacity to induce anti-inflammatory IL-10 secretion.					
30678156	3	76	theme	PYC	389:391	arg1	activity					377:384	the immunomodulatory activity	356:384	the immunomodulatory activity of PYC	356:391	However, the role of the other receptors from the TLR family in the immunomodulatory activity of PYC has not been described so far.					
30678156	10	77	contain	containing	1245:1254	arg1	fraction					1236:1243	The dialyzed fraction	1223:1243	The dialyzed fraction containing PYC metabolites	1223:1270	The dialyzed fraction containing PYC metabolites revealed the capacity to induce anti-inflammatory IL-10 secretion.					
30678156	10	77	contain	containing	1245:1254	arg2	metabolites					1260:1270	PYC metabolites	1256:1270	PYC metabolites	1256:1270	The dialyzed fraction containing PYC metabolites revealed the capacity to induce anti-inflammatory IL-10 secretion.					
30678156	12	78	theme	signaling	1599:1607	arg1	inhibitor					1581:1589	a potential inhibitor	1569:1589	a potential inhibitor of TLRs signaling	1569:1607	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	12	78	theme	signaling	1599:1607	arg1	activity					1557:1564	its biological activity	1542:1564	its biological activity	1542:1564	CONCLUSIONS This study showed that gastrointestinal metabolism of PYC reveals its biological activity as a potential inhibitor of TLRs signaling.					
30678156	4	79	theme	immunomodulatory	497:512	arg1	properties					514:523	its immunomodulatory properties	493:523	its immunomodulatory properties	493:523	AIM The aim of this study was to investigate whether PYC might exert its immunomodulatory properties through cell membrane TLRs (TLR1/2, TLR5, and TLR2/6) other than TLR4.					
30678156	11	80	theme	colonic	1389:1395	arg1	composition					1408:1418	the colonic microbiota composition	1385:1418	the colonic microbiota composition	1385:1418	Finally, microbially metabolized PYC affected the colonic microbiota composition during in vitro gastrointestinal digestion.					
30678156	0	81	theme	Pycnogenol®	58:68	arg1	Activity					46:53	Toll-Like Receptor-Dependent Immunomodulatory Activity	0:53	Toll-Like Receptor-Dependent Immunomodulatory Activity of Pycnogenol®	0:68	Toll-Like Receptor-Dependent Immunomodulatory Activity of Pycnogenol®.					
30678156	6	82	theme	TLR1/2	795:800	arg1	agonist					784:790	an agonist	781:790	an agonist of TLR1/2 and TLR2/6	781:811	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	6	82	theme	TLR1/2	795:800	arg1	PYC					752:754	intact non-metabolized PYC	729:754	intact non-metabolized PYC	729:754	FINDINGS We showed that intact non-metabolized PYC dose-dependently acts as an agonist of TLR1/2 and TLR2/6 and as a partial agonist of TLR5.					
30678156	9	83	theme	Gastrointestinal	1017:1032	arg1	metabolism					1034:1043	Gastrointestinal metabolism	1017:1043	Gastrointestinal metabolism of PYC	1017:1050	Gastrointestinal metabolism of PYC revealed the immunosuppressive potential of the retentate fraction against TLR1/2 and TLR2/6 when compared to the control fraction containing microbiota and enzymes only.					
30678156	8	84	theme	TLR4	1001:1004	arg1	signaling					1006:1014	TLR4 signaling	1001:1014	TLR4 signaling	1001:1014	However, after the formation of complexes with lipopolysaccharides (LPS), it is a potent activator of TLR4 signaling.					
30678156	13	85	link	microbiota-derived	1721:1738	arg1	agonists					1744:1751	the microbiota-derived TLR agonists	1717:1751	the microbiota-derived TLR agonists (retentate fraction)	1717:1772	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
30678156	13	85	link	microbiota-derived	1721:1738	arg1	fraction					1764:1771	retentate fraction	1754:1771	retentate fraction	1754:1771	The results suggest that metabolized PYC acts as a partial agonist of TLR1/2 and TLR2/6 in the presence of the microbiota-derived TLR agonists (retentate fraction) and that it possesses anti-inflammatory potential reflected by the induction of IL-10 from THP-1 macrophages (dialysate fraction).					
31103595	0	0	theme	dye	75:77	arg1	removal					79:85	efficient dye removal	65:85	efficient dye removal from aqueous solution	65:107	An adsorbent based on humic acid and carboxymethyl cellulose for efficient dye removal from aqueous solution.					
31103595	2	1	theme	calcium	332:338	arg1	PCH					351:353	PCH	351:353	PCH	351:353	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	2	1	theme	calcium	332:338	arg1	hydroxide					340:348	calcium hydroxide	332:348	calcium hydroxide (PCH)	332:354	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	6	2	theme	behavior	945:952	arg1	analysis					954:961	Adsorption behavior analysis	934:961	Adsorption behavior analysis	934:961	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	0	3	theme	efficient	65:73	arg1	removal					79:85	efficient dye removal	65:85	efficient dye removal from aqueous solution	65:107	An adsorbent based on humic acid and carboxymethyl cellulose for efficient dye removal from aqueous solution.					
31103595	4	4	theme	organic	635:641	arg1	pollutant					643:651	a typical organic pollutant	625:651	a typical organic pollutant of methylene blue (MB) removal from aqueous solution	625:704	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	6	5	theme	Adsorption	934:943	arg1	analysis					954:961	Adsorption behavior analysis	934:961	Adsorption behavior analysis	934:961	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	4	6	theme	high	709:712	arg1	capacity					725:732	a high adsorption capacity	707:732	a high adsorption capacity of 666.67 mg/g	707:747	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	8	7	theme	adsorption	1307:1316	arg1	capacity					1318:1325	adsorption capacity remaining	1307:1335	adsorption capacity remaining in the fifth recycle runs	1307:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	4	8	from	solution	697:704	arg1	removal					676:682	methylene blue (MB) removal	656:682	methylene blue (MB) removal from aqueous solution	656:704	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	4	8	from	solution	697:704	arg1	pollutant					643:651	a typical organic pollutant	625:651	a typical organic pollutant of methylene blue (MB) removal from aqueous solution	625:704	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	5	9	theme	exchanging	824:833	arg1	process					873:879	this adsorption process	857:879	this adsorption process mainly occurred on both anionic and aromatic groups	857:931	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	5	9	theme	exchanging	824:833	arg1	interactions					843:854	ion exchanging and π-π interactions	820:854	ion exchanging and π-π interactions	820:854	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	8	10	theme	fifth	1344:1348	arg1	runs					1358:1361	the fifth recycle runs	1340:1361	the fifth recycle runs	1340:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	5	11	theme	anionic	905:911	arg1	groups					926:931	both anionic and aromatic groups	900:931	both anionic and aromatic groups	900:931	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	1	12	from	precursors	138:147	arg1	Adsorbents					110:119	Adsorbents	110:119	Adsorbents from sustainable precursors	110:147	Adsorbents from sustainable precursors are highly desirable for practical applications.					
31103595	4	13	theme	aqueous	689:695	arg1	solution					697:704	aqueous solution	689:704	aqueous solution	689:704	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	4	14	theme	blue	666:669	arg1	methylene					656:664	methylene blue	656:669	methylene blue (MB) removal from aqueous solution	656:704	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	4	14	theme	blue	666:669	arg1	MB					672:673	MB	672:673	MB	672:673	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	9	15	theme	adsorption	1448:1457	arg1	applications					1459:1470	adsorption applications	1448:1470	adsorption applications	1448:1470	Therefore, the adsorbent of HA-CMC/PMH is suggested to be a promising candidate for adsorption applications.					
31103595	9	16	theme	promising	1424:1432	arg1	adsorbent					1379:1387	adsorbent	1379:1387	adsorbent	1379:1387	Therefore, the adsorbent of HA-CMC/PMH is suggested to be a promising candidate for adsorption applications.					
31103595	9	16	theme	promising	1424:1432	arg1	candidate					1434:1442	a promising candidate	1422:1442	a promising candidate for adsorption applications	1422:1470	Therefore, the adsorbent of HA-CMC/PMH is suggested to be a promising candidate for adsorption applications.					
31103595	8	17	theme	remaining	1327:1335	arg1	capacity					1318:1325	adsorption capacity remaining	1307:1335	adsorption capacity remaining in the fifth recycle runs	1307:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	6	18	theme	kinetic	1026:1032	arg1	model					1034:1038	pseudo-second order kinetic model	1006:1038	pseudo-second order kinetic model	1006:1038	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	2	19	theme	ferric	360:365	arg1	PFH					378:380	PFH	378:380	PFH	378:380	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	2	19	theme	ferric	360:365	arg1	hydroxide					367:375	ferric hydroxide	360:375	ferric hydroxide (PFH)	360:381	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	0	20	theme	aqueous	92:98	arg1	solution					100:107	aqueous solution	92:107	aqueous solution	92:107	An adsorbent based on humic acid and carboxymethyl cellulose for efficient dye removal from aqueous solution.					
31103595	5	21	theme	π-π	839:841	arg1	process					873:879	this adsorption process	857:879	this adsorption process mainly occurred on both anionic and aromatic groups	857:931	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	5	21	theme	π-π	839:841	arg1	interactions					843:854	ion exchanging and π-π interactions	820:854	ion exchanging and π-π interactions	820:854	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	8	22	from	%	1302:1302	arg1	runs					1358:1361	the fifth recycle runs	1340:1361	the fifth recycle runs	1340:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	5	23	theme	aromatic	917:924	arg1	groups					926:931	both anionic and aromatic groups	900:931	both anionic and aromatic groups	900:931	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	6	24	theme	experimental	1047:1058	arg1	data					1060:1063	experimental data	1047:1063	experimental data	1047:1063	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	1	25	theme	practical	174:182	arg1	applications					184:195	practical applications	174:195	practical applications	174:195	Adsorbents from sustainable precursors are highly desirable for practical applications.					
31103595	7	26	theme	Further	1132:1138	arg1	analysis					1154:1161	Further thermodynamic analysis	1132:1161	Further thermodynamic analysis	1132:1161	Further thermodynamic analysis proved that the adsorption of MB onto HA-CMC/PMH was an exothermal spontaneous process.					
31103595	3	27	theme	infrared	488:495	arg1	spectroscopy					497:508	infrared spectroscopy	488:508	infrared spectroscopy	488:508	The as-obtained adsorbent, denoted as HA-CMC/PMH (PCH and PFH were referred to as PMH), was analyzed by infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and thermo gravimetric analysis.					
31103595	8	28	from	runs	1358:1361	arg1	capacity					1318:1325	adsorption capacity remaining	1307:1335	adsorption capacity remaining in the fifth recycle runs	1307:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	8	28	from	runs	1358:1361	arg1	%					1302:1302	79.93%	1297:1302	79.93% of adsorption capacity remaining in the fifth recycle runs	1297:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	5	29	theme	MB	808:809	arg1	mechanisms					794:803	The adsorption mechanisms	779:803	The adsorption mechanisms of MB	779:809	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	7	30	theme	thermodynamic	1140:1152	arg1	analysis					1154:1161	Further thermodynamic analysis	1132:1161	Further thermodynamic analysis	1132:1161	Further thermodynamic analysis proved that the adsorption of MB onto HA-CMC/PMH was an exothermal spontaneous process.					
31103595	8	31	with	reusable	1283:1290	arg1	capacity					1318:1325	adsorption capacity remaining	1307:1335	adsorption capacity remaining in the fifth recycle runs	1307:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	8	31	with	reusable	1283:1290	arg1	%					1302:1302	79.93%	1297:1302	79.93% of adsorption capacity remaining in the fifth recycle runs	1297:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	6	32	theme	order	1020:1024	arg1	model					1034:1038	pseudo-second order kinetic model	1006:1038	pseudo-second order kinetic model	1006:1038	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	2	33	theme	carboxymethyl	213:225	arg1	CMC					238:240	CMC	238:240	CMC	238:240	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	2	33	theme	carboxymethyl	213:225	arg1	cellulose					227:235	carboxymethyl cellulose	213:235	carboxymethyl cellulose (CMC)	213:241	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	6	34	theme	MB	1109:1110	arg1	adsorption					1095:1104	a mono-layer adsorption	1082:1104	a mono-layer adsorption of MB onto the adsorbent	1082:1129	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	6	35	theme	pseudo-second	1006:1018	arg1	model					1034:1038	pseudo-second order kinetic model	1006:1038	pseudo-second order kinetic model	1006:1038	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	3	36	theme	gravimetric	585:595	arg1	analysis					597:604	thermo gravimetric analysis	578:604	thermo gravimetric analysis	578:604	The as-obtained adsorbent, denoted as HA-CMC/PMH (PCH and PFH were referred to as PMH), was analyzed by infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and thermo gravimetric analysis.					
31103595	4	37	theme	removal	676:682	arg1	pollutant					643:651	a typical organic pollutant	625:651	a typical organic pollutant of methylene blue (MB) removal from aqueous solution	625:704	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	9	38	theme	HA-CMC/PMH	1392:1401	arg1	adsorbent					1379:1387	adsorbent	1379:1387	adsorbent	1379:1387	Therefore, the adsorbent of HA-CMC/PMH is suggested to be a promising candidate for adsorption applications.					
31103595	9	38	theme	HA-CMC/PMH	1392:1401	arg1	candidate					1434:1442	a promising candidate	1422:1442	a promising candidate for adsorption applications	1422:1470	Therefore, the adsorbent of HA-CMC/PMH is suggested to be a promising candidate for adsorption applications.					
31103595	5	39	theme	ion	820:822	arg1	process					873:879	this adsorption process	857:879	this adsorption process mainly occurred on both anionic and aromatic groups	857:931	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	5	39	theme	ion	820:822	arg1	interactions					843:854	ion exchanging and π-π interactions	820:854	ion exchanging and π-π interactions	820:854	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	3	40	theme	electron	520:527	arg1	microscopy					529:538	scanning electron microscopy	511:538	scanning electron microscopy	511:538	The as-obtained adsorbent, denoted as HA-CMC/PMH (PCH and PFH were referred to as PMH), was analyzed by infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and thermo gravimetric analysis.					
31103595	0	41	theme	humic	22:26	arg1	acid					28:31	humic acid	22:31	humic acid	22:31	An adsorbent based on humic acid and carboxymethyl cellulose for efficient dye removal from aqueous solution.					
31103595	4	42	theme	666.67 mg/g	737:747	arg1	capacity					725:732	a high adsorption capacity	707:732	a high adsorption capacity of 666.67 mg/g	707:747	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	2	43	theme	hydroxide	367:375	arg1	precipitates					316:327	precipitates	316:327	precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH)	316:381	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	0	44	theme	carboxymethyl	37:49	arg1	cellulose					51:59	carboxymethyl cellulose	37:59	carboxymethyl cellulose	37:59	An adsorbent based on humic acid and carboxymethyl cellulose for efficient dye removal from aqueous solution.					
31103595	6	45	theme	isotherm	987:994	arg1	model					996:1000	Langmuir isotherm model	978:1000	Langmuir isotherm model	978:1000	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	3	46	theme	thermo	578:583	arg1	analysis					597:604	thermo gravimetric analysis	578:604	thermo gravimetric analysis	578:604	The as-obtained adsorbent, denoted as HA-CMC/PMH (PCH and PFH were referred to as PMH), was analyzed by infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and thermo gravimetric analysis.					
31103595	4	47	theme	methylene	656:664	arg1	removal					676:682	methylene blue (MB) removal	656:682	methylene blue (MB) removal from aqueous solution	656:704	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	0	48	from	solution	100:107	arg1	removal					79:85	efficient dye removal	65:85	efficient dye removal from aqueous solution	65:107	An adsorbent based on humic acid and carboxymethyl cellulose for efficient dye removal from aqueous solution.					
31103595	3	49	theme	scanning	511:518	arg1	microscopy					529:538	scanning electron microscopy	511:538	scanning electron microscopy	511:538	The as-obtained adsorbent, denoted as HA-CMC/PMH (PCH and PFH were referred to as PMH), was analyzed by infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and thermo gravimetric analysis.					
31103595	6	50	theme	mono-layer	1084:1093	arg1	adsorption					1095:1104	a mono-layer adsorption	1082:1104	a mono-layer adsorption of MB onto the adsorbent	1082:1129	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	7	51	theme	exothermal	1219:1228	arg1	process					1242:1248	an exothermal spontaneous process	1216:1248	an exothermal spontaneous process	1216:1248	Further thermodynamic analysis proved that the adsorption of MB onto HA-CMC/PMH was an exothermal spontaneous process.					
31103595	7	51	theme	exothermal	1219:1228	arg1	adsorption					1179:1188	the adsorption	1175:1188	the adsorption of MB onto HA-CMC/PMH	1175:1210	Further thermodynamic analysis proved that the adsorption of MB onto HA-CMC/PMH was an exothermal spontaneous process.					
31103595	4	52	theme	adsorption	714:723	arg1	capacity					725:732	a high adsorption capacity	707:732	a high adsorption capacity of 666.67 mg/g	707:747	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	3	53	theme	photoelectron	547:559	arg1	spectroscopy					561:572	X-ray photoelectron spectroscopy	541:572	X-ray photoelectron spectroscopy	541:572	The as-obtained adsorbent, denoted as HA-CMC/PMH (PCH and PFH were referred to as PMH), was analyzed by infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and thermo gravimetric analysis.					
31103595	7	54	theme	spontaneous	1230:1240	arg1	process					1242:1248	an exothermal spontaneous process	1216:1248	an exothermal spontaneous process	1216:1248	Further thermodynamic analysis proved that the adsorption of MB onto HA-CMC/PMH was an exothermal spontaneous process.					
31103595	7	54	theme	spontaneous	1230:1240	arg1	adsorption					1179:1188	the adsorption	1175:1188	the adsorption of MB onto HA-CMC/PMH	1175:1210	Further thermodynamic analysis proved that the adsorption of MB onto HA-CMC/PMH was an exothermal spontaneous process.					
31103595	8	55	theme	recycle	1350:1356	arg1	runs					1358:1361	the fifth recycle runs	1340:1361	the fifth recycle runs	1340:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	6	56	theme	Langmuir	978:985	arg1	model					996:1000	Langmuir isotherm model	978:1000	Langmuir isotherm model	978:1000	Adsorption behavior analysis indicated that Langmuir isotherm model and pseudo-second order kinetic model fitted experimental data well, suggesting a mono-layer adsorption of MB onto the adsorbent.					
31103595	8	57	theme	capacity	1318:1325	arg1	capacity					1318:1325	adsorption capacity remaining	1307:1335	adsorption capacity remaining in the fifth recycle runs	1307:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	8	57	theme	capacity	1318:1325	arg1	%					1302:1302	79.93%	1297:1302	79.93% of adsorption capacity remaining in the fifth recycle runs	1297:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	2	58	theme	humic	247:251	arg1	HA					259:260	HA	259:260	HA	259:260	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	2	58	theme	humic	247:251	arg1	acid					253:256	humic acid	247:256	humic acid (HA)	247:261	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	1	59	theme	sustainable	126:136	arg1	precursors					138:147	sustainable precursors	126:147	sustainable precursors	126:147	Adsorbents from sustainable precursors are highly desirable for practical applications.					
31103595	5	60	theme	adsorption	862:871	arg1	process					873:879	this adsorption process	857:879	this adsorption process mainly occurred on both anionic and aromatic groups	857:931	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	5	60	theme	adsorption	862:871	arg1	interactions					843:854	ion exchanging and π-π interactions	820:854	ion exchanging and π-π interactions	820:854	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	7	61	theme	MB	1193:1194	arg1	process					1242:1248	an exothermal spontaneous process	1216:1248	an exothermal spontaneous process	1216:1248	Further thermodynamic analysis proved that the adsorption of MB onto HA-CMC/PMH was an exothermal spontaneous process.					
31103595	7	61	theme	MB	1193:1194	arg1	adsorption					1179:1188	the adsorption	1175:1188	the adsorption of MB onto HA-CMC/PMH	1175:1210	Further thermodynamic analysis proved that the adsorption of MB onto HA-CMC/PMH was an exothermal spontaneous process.					
31103595	5	62	theme	adsorption	783:792	arg1	mechanisms					794:803	The adsorption mechanisms	779:803	The adsorption mechanisms of MB	779:809	The adsorption mechanisms of MB involved ion exchanging and π-π interactions, this adsorption process mainly occurred on both anionic and aromatic groups.					
31103595	4	63	theme	typical	627:633	arg1	pollutant					643:651	a typical organic pollutant	625:651	a typical organic pollutant of methylene blue (MB) removal from aqueous solution	625:704	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31103595	8	64	from	capacity	1318:1325	arg1	runs					1358:1361	the fifth recycle runs	1340:1361	the fifth recycle runs	1340:1361	The developed adsorbent is also reusable with 79.93% of adsorption capacity remaining in the fifth recycle runs.					
31103595	3	65	theme	X-ray	541:545	arg1	spectroscopy					561:572	X-ray photoelectron spectroscopy	541:572	X-ray photoelectron spectroscopy	541:572	The as-obtained adsorbent, denoted as HA-CMC/PMH (PCH and PFH were referred to as PMH), was analyzed by infrared spectroscopy, scanning electron microscopy, X-ray photoelectron spectroscopy and thermo gravimetric analysis.					
31103595	2	66	theme	hydroxide	340:348	arg1	precipitates					316:327	precipitates	316:327	precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH)	316:381	In this study, carboxymethyl cellulose (CMC) and humic acid (HA) were adopted to fabricate a composite immobilized on precipitates of calcium hydroxide (PCH) and ferric hydroxide (PFH).					
31103595	4	67	from	pollutant	643:651	arg1	solution					697:704	aqueous solution	689:704	aqueous solution	689:704	When applying for a typical organic pollutant of methylene blue (MB) removal from aqueous solution, a high adsorption capacity of 666.67 mg/g was achieved over HA-CMC/PMH.					
31683441	11	0	theme	great	1719:1723	arg1	potential					1725:1733	great potential	1719:1733	great potential	1719:1733	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	7	1	theme	adsorption	1055:1064	arg1	capacity					1066:1073	the adsorption capacity	1051:1073	the adsorption capacity of the NH2-DN for Cu2+ and CIP	1051:1104	Compared with that of an ordinary graphene/alginate single-network hydrogel (SN), the adsorption capacity of the NH2-DN for Cu2+ and CIP reached 153.91 mg g-1 and 301.36 mg g-1, respectively, which was increased by 130% and 182%, respectively.					
31683441	0	2	theme	aqueous	107:113	arg1	solution					115:122	aqueous solution	107:122	aqueous solution	107:122	Amino-functionalized alginate/graphene double-network hydrogel beads for emerging contaminant removal from aqueous solution.					
31683441	9	3	theme	Adsorption	1348:1357	arg1	properties					1359:1368	Adsorption properties	1348:1368	Adsorption properties	1348:1368	Adsorption properties were comprehensively examined, including the effects of the solid-liquid ratios, pH, and ionic strength.					
31683441	6	4	theme	typical	907:913	arg1	ciprofloxacin					947:959	ciprofloxacin	947:959	ciprofloxacin	947:959	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	6	4	theme	typical	907:913	arg1	Cu2+					938:941	Cu2+	938:941	Cu2+	938:941	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	6	4	theme	typical	907:913	arg1	contaminants					924:935	typical emerging contaminants	907:935	typical emerging contaminants (Cu2+ and ciprofloxacin (CIP))	907:966	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	3	5	theme	macropore	570:578	arg1	structures					580:589	mesopore and macropore structures	557:589	mesopore and macropore structures on the hydrogel surface	557:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	8	6	theme	Adsorption	1213:1222	arg1	isotherm					1224:1231	Adsorption isotherm and kinetic analyses	1213:1252	isotherm	1224:1231	Adsorption isotherm and kinetic analyses reveal that the adsorption process of CIP onto the NH2-DN was dominated by chemical affinity.					
31683441	3	7	used	used	411:414	arg2	TETA					401:404	TETA	401:404	TETA	401:404	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	3	7	used	used	411:414	arg2	Triethylenetetramine					379:398	Triethylenetetramine	379:398	Triethylenetetramine (TETA)	379:405	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	3	7	used	used	411:414	arg2	crosslinker					421:431	a crosslinker	419:431	a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface	419:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	4	8	theme	thermal	662:668	arg1	stability					670:678	enhanced thermal stability	653:678	enhanced thermal stability	653:678	Compared to single network hydrogel, enhanced thermal stability and mechanical properties were achieved in NH2-DN.					
31683441	10	9	theme	alkaline	1636:1643	arg1	solutions					1645:1653	weak acidic and alkaline solutions	1620:1653	weak acidic and alkaline solutions	1620:1653	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	11	10	theme	environmental	1738:1750	arg1	applications					1752:1763	environmental applications	1738:1763	environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater	1738:1883	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	3	11	from	structures	580:589	arg1	surface					607:613	the hydrogel surface	594:613	the hydrogel surface	594:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	11	12	theme	organic-inorganic	1688:1704	arg1	hydrogel					1706:1713	This novel amino-functionalized organic-inorganic hydrogel	1656:1713	This novel amino-functionalized organic-inorganic hydrogel	1656:1713	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	4	13	theme	enhanced	653:660	arg1	stability					670:678	enhanced thermal stability	653:678	enhanced thermal stability	653:678	Compared to single network hydrogel, enhanced thermal stability and mechanical properties were achieved in NH2-DN.					
31683441	7	14	dep	reached	1106:1112	arg1	increased					1171:1179	increased	1171:1179	was increased by 130% and 182%, respectively	1167:1210	Compared with that of an ordinary graphene/alginate single-network hydrogel (SN), the adsorption capacity of the NH2-DN for Cu2+ and CIP reached 153.91 mg g-1 and 301.36 mg g-1, respectively, which was increased by 130% and 182%, respectively.					
31683441	3	15	dep	sheets	474:479	arg1	stacking					481:488	stacking	481:488	stacking	481:488	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	3	15	dep	sheets	474:479	arg1	resulted					494:501	resulted	494:501	resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface	494:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	6	16	theme	adsorption	881:890	arg1	properties					892:901	excellent adsorption properties	871:901	excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP))	871:966	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	3	17	theme	reduced	508:514	arg1	network					537:543	a reduced graphene oxide (rGO) network	506:543	a reduced graphene oxide (rGO) network	506:543	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	10	18	theme	adsorption	1597:1606	arg1	capacity					1608:1615	its adsorption capacity	1593:1615	its adsorption capacity	1593:1615	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	11	19	theme	adsorption	1823:1832	arg1	properties					1834:1843	its outstanding physicochemical, mechanical, and adsorption properties	1774:1843	properties	1834:1843	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	10	20	dep	acidic	1625:1630	arg1	weak					1620:1623	weak	1620:1623	weak	1620:1623	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	5	21	theme	elasticity	735:744	arg1	modulus					746:752	The elasticity modulus	731:752	The elasticity modulus	731:752	The elasticity modulus was improved by approximately 3 times due to the formation of the double-network.					
31683441	2	22	theme	adsorption	330:339	arg1	properties					341:350	excellent mechanical and adsorption properties	305:350	properties	341:350	In this study, an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties was successfully prepared.					
31683441	3	23	theme	graphene	516:523	arg1	rGO					532:534	rGO	532:534	rGO	532:534	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	3	23	theme	graphene	516:523	arg1	oxide					525:529	graphene oxide	516:529	a reduced graphene oxide (rGO) network	506:543	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	0	24	theme	alginate/graphene	21:37	arg1	beads					63:67	alginate/graphene double-network hydrogel beads	21:67	alginate/graphene double-network hydrogel beads	21:67	Amino-functionalized alginate/graphene double-network hydrogel beads for emerging contaminant removal from aqueous solution.					
31683441	3	25	contain	containing	546:555	arg1	network					537:543	a reduced graphene oxide (rGO) network	506:543	a reduced graphene oxide (rGO) network	506:543	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	3	25	contain	containing	546:555	arg2	structures					580:589	mesopore and macropore structures	557:589	mesopore and macropore structures on the hydrogel surface	557:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	10	26	theme	capacity	1513:1520	arg1	capacity					1513:1520	its adsorption capacity	1498:1520	its adsorption capacity	1498:1520	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	10	26	theme	capacity	1513:1520	arg1	%					1493:1493	94%	1491:1493	94% of its adsorption capacity	1491:1520	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	3	27	theme	graphene	465:472	arg1	sheets					474:479	random few-layer graphene sheets	448:479	random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface	448:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	6	28	dep	contaminants	924:935	arg1	ciprofloxacin					947:959	ciprofloxacin	947:959	ciprofloxacin	947:959	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	6	28	dep	contaminants	924:935	arg1	Cu2+					938:941	Cu2+	938:941	Cu2+	938:941	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	6	28	dep	contaminants	924:935	arg1	contaminants					924:935	typical emerging contaminants	907:935	typical emerging contaminants (Cu2+ and ciprofloxacin (CIP))	907:966	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	6	28	dep	contaminants	924:935	arg1	CIP					962:964	CIP	962:964	CIP	962:964	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	10	29	theme	adsorption	1502:1511	arg1	capacity					1513:1520	its adsorption capacity	1498:1520	its adsorption capacity	1498:1520	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	7	30	theme	ordinary	994:1001	arg1	SN					1046:1047	SN	1046:1047	SN	1046:1047	Compared with that of an ordinary graphene/alginate single-network hydrogel (SN), the adsorption capacity of the NH2-DN for Cu2+ and CIP reached 153.91 mg g-1 and 301.36 mg g-1, respectively, which was increased by 130% and 182%, respectively.					
31683441	7	30	theme	ordinary	994:1001	arg1	hydrogel					1036:1043	an ordinary graphene/alginate single-network hydrogel	991:1043	an ordinary graphene/alginate single-network hydrogel (SN)	991:1048	Compared with that of an ordinary graphene/alginate single-network hydrogel (SN), the adsorption capacity of the NH2-DN for Cu2+ and CIP reached 153.91 mg g-1 and 301.36 mg g-1, respectively, which was increased by 130% and 182%, respectively.					
31683441	1	31	theme	Inorganic-organic	125:141	arg1	hydrogels					153:161	Inorganic-organic composite hydrogels	125:161	Inorganic-organic composite hydrogels	125:161	Inorganic-organic composite hydrogels have attracted much attention in recent years.					
31683441	6	32	theme	excellent	871:879	arg1	properties					892:901	excellent adsorption properties	871:901	excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP))	871:966	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	9	33	theme	ionic	1459:1463	arg1	strength					1465:1472	ionic strength	1459:1472	ionic strength	1459:1472	Adsorption properties were comprehensively examined, including the effects of the solid-liquid ratios, pH, and ionic strength.					
31683441	0	34	theme	hydrogel	54:61	arg1	beads					63:67	alginate/graphene double-network hydrogel beads	21:67	alginate/graphene double-network hydrogel beads	21:67	Amino-functionalized alginate/graphene double-network hydrogel beads for emerging contaminant removal from aqueous solution.					
31683441	2	35	with	hydrogel	282:289	arg1	mechanical					315:324	mechanical	315:324	mechanical	315:324	In this study, an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties was successfully prepared.					
31683441	2	35	with	hydrogel	282:289	arg1	properties					341:350	excellent mechanical and adsorption properties	305:350	properties	341:350	In this study, an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties was successfully prepared.					
31683441	7	36	theme	graphene/alginate	1003:1019	arg1	SN					1046:1047	SN	1046:1047	SN	1046:1047	Compared with that of an ordinary graphene/alginate single-network hydrogel (SN), the adsorption capacity of the NH2-DN for Cu2+ and CIP reached 153.91 mg g-1 and 301.36 mg g-1, respectively, which was increased by 130% and 182%, respectively.					
31683441	7	36	theme	graphene/alginate	1003:1019	arg1	hydrogel					1036:1043	an ordinary graphene/alginate single-network hydrogel	991:1043	an ordinary graphene/alginate single-network hydrogel (SN)	991:1048	Compared with that of an ordinary graphene/alginate single-network hydrogel (SN), the adsorption capacity of the NH2-DN for Cu2+ and CIP reached 153.91 mg g-1 and 301.36 mg g-1, respectively, which was increased by 130% and 182%, respectively.					
31683441	1	37	theme	composite	143:151	arg1	hydrogels					153:161	Inorganic-organic composite hydrogels	125:161	Inorganic-organic composite hydrogels	125:161	Inorganic-organic composite hydrogels have attracted much attention in recent years.					
31683441	0	38	theme	double-network	39:52	arg1	beads					63:67	alginate/graphene double-network hydrogel beads	21:67	alginate/graphene double-network hydrogel beads	21:67	Amino-functionalized alginate/graphene double-network hydrogel beads for emerging contaminant removal from aqueous solution.					
31683441	4	39	theme	network	635:641	arg1	hydrogel					643:650	single network hydrogel	628:650	single network hydrogel	628:650	Compared to single network hydrogel, enhanced thermal stability and mechanical properties were achieved in NH2-DN.					
31683441	11	40	from	contaminants	1858:1869	arg1	wastewater					1874:1883	wastewater	1874:1883	wastewater	1874:1883	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	0	41	from	solution	115:122	arg1	removal					94:100	emerging contaminant removal	73:100	emerging contaminant removal from aqueous solution	73:122	Amino-functionalized alginate/graphene double-network hydrogel beads for emerging contaminant removal from aqueous solution.					
31683441	8	42	theme	adsorption	1270:1279	arg1	process					1281:1287	the adsorption process	1266:1287	the adsorption process of CIP onto the NH2-DN	1266:1310	Adsorption isotherm and kinetic analyses reveal that the adsorption process of CIP onto the NH2-DN was dominated by chemical affinity.					
31683441	4	43	theme	single	628:633	arg1	hydrogel					643:650	single network hydrogel	628:650	single network hydrogel	628:650	Compared to single network hydrogel, enhanced thermal stability and mechanical properties were achieved in NH2-DN.					
31683441	5	44	theme	due	792:794	arg1	times					786:790	approximately 3 times	770:790	approximately 3 times due to the formation of the double-network	770:833	The elasticity modulus was improved by approximately 3 times due to the formation of the double-network.					
31683441	8	45	theme	chemical	1329:1336	arg1	affinity					1338:1345	chemical affinity	1329:1345	chemical affinity	1329:1345	Adsorption isotherm and kinetic analyses reveal that the adsorption process of CIP onto the NH2-DN was dominated by chemical affinity.					
31683441	11	46	contain	has	1715:1717	arg1	hydrogel					1706:1713	This novel amino-functionalized organic-inorganic hydrogel	1656:1713	This novel amino-functionalized organic-inorganic hydrogel	1656:1713	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	11	46	contain	has	1715:1717	arg2	potential					1725:1733	great potential	1719:1733	great potential	1719:1733	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	3	47	theme	oxide	525:529	arg1	network					537:543	a reduced graphene oxide (rGO) network	506:543	a reduced graphene oxide (rGO) network	506:543	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	10	48	theme	acidic	1625:1630	arg1	solutions					1645:1653	weak acidic and alkaline solutions	1620:1653	weak acidic and alkaline solutions	1620:1653	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	10	49	theme	ionic	1531:1535	arg1	0.5 mol L-1					1550:1560	0.5 mol L-1	1550:1560	0.5 mol L-1	1550:1560	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	10	49	theme	ionic	1531:1535	arg1	strength					1537:1544	the ionic strength	1527:1544	the ionic strength	1527:1544	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	0	50	theme	contaminant	82:92	arg1	removal					94:100	emerging contaminant removal	73:100	emerging contaminant removal from aqueous solution	73:122	Amino-functionalized alginate/graphene double-network hydrogel beads for emerging contaminant removal from aqueous solution.					
31683441	9	51	theme	solid-liquid	1430:1441	arg1	ratios					1443:1448	the solid-liquid ratios	1426:1448	the solid-liquid ratios	1426:1448	Adsorption properties were comprehensively examined, including the effects of the solid-liquid ratios, pH, and ionic strength.					
31683441	0	52	theme	emerging	73:80	arg1	removal					94:100	emerging contaminant removal	73:100	emerging contaminant removal from aqueous solution	73:122	Amino-functionalized alginate/graphene double-network hydrogel beads for emerging contaminant removal from aqueous solution.					
31683441	9	53	theme	ratios	1443:1448	arg1	effects					1415:1421	the effects	1411:1421	the effects of the solid-liquid ratios	1411:1448	Adsorption properties were comprehensively examined, including the effects of the solid-liquid ratios, pH, and ionic strength.					
31683441	9	53	theme	ratios	1443:1448	arg1	pH					1451:1452	pH	1451:1452	pH	1451:1452	Adsorption properties were comprehensively examined, including the effects of the solid-liquid ratios, pH, and ionic strength.					
31683441	9	53	theme	ratios	1443:1448	arg1	strength					1465:1472	ionic strength	1459:1472	ionic strength	1459:1472	Adsorption properties were comprehensively examined, including the effects of the solid-liquid ratios, pH, and ionic strength.					
31683441	2	54	theme	double-network	267:280	arg1	NH2-DN					292:297	NH2-DN	292:297	NH2-DN	292:297	In this study, an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties was successfully prepared.					
31683441	2	54	theme	double-network	267:280	arg1	hydrogel					282:289	an amino-functionalized graphene/alginate double-network hydrogel	225:289	an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties	225:350	In this study, an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties was successfully prepared.					
31683441	7	55	theme	single-network	1021:1034	arg1	SN					1046:1047	SN	1046:1047	SN	1046:1047	Compared with that of an ordinary graphene/alginate single-network hydrogel (SN), the adsorption capacity of the NH2-DN for Cu2+ and CIP reached 153.91 mg g-1 and 301.36 mg g-1, respectively, which was increased by 130% and 182%, respectively.					
31683441	7	55	theme	single-network	1021:1034	arg1	hydrogel					1036:1043	an ordinary graphene/alginate single-network hydrogel	991:1043	an ordinary graphene/alginate single-network hydrogel (SN)	991:1048	Compared with that of an ordinary graphene/alginate single-network hydrogel (SN), the adsorption capacity of the NH2-DN for Cu2+ and CIP reached 153.91 mg g-1 and 301.36 mg g-1, respectively, which was increased by 130% and 182%, respectively.					
31683441	3	56	theme	mesopore	557:564	arg1	structures					580:589	mesopore and macropore structures	557:589	mesopore and macropore structures on the hydrogel surface	557:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	2	57	theme	graphene/alginate	249:265	arg1	NH2-DN					292:297	NH2-DN	292:297	NH2-DN	292:297	In this study, an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties was successfully prepared.					
31683441	2	57	theme	graphene/alginate	249:265	arg1	hydrogel					282:289	an amino-functionalized graphene/alginate double-network hydrogel	225:289	an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties	225:350	In this study, an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties was successfully prepared.					
31683441	7	58	theme	NH2-DN	1082:1087	arg1	capacity					1066:1073	the adsorption capacity	1051:1073	the adsorption capacity of the NH2-DN for Cu2+ and CIP	1051:1104	Compared with that of an ordinary graphene/alginate single-network hydrogel (SN), the adsorption capacity of the NH2-DN for Cu2+ and CIP reached 153.91 mg g-1 and 301.36 mg g-1, respectively, which was increased by 130% and 182%, respectively.					
31683441	1	59	theme	much	178:181	arg1	attention					183:191	much attention	178:191	much attention	178:191	Inorganic-organic composite hydrogels have attracted much attention in recent years.					
31683441	3	60	theme	hydrogel	598:605	arg1	surface					607:613	the hydrogel surface	594:613	the hydrogel surface	594:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	2	61	theme	amino-functionalized	228:247	arg1	NH2-DN					292:297	NH2-DN	292:297	NH2-DN	292:297	In this study, an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties was successfully prepared.					
31683441	2	61	theme	amino-functionalized	228:247	arg1	hydrogel					282:289	an amino-functionalized graphene/alginate double-network hydrogel	225:289	an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties	225:350	In this study, an amino-functionalized graphene/alginate double-network hydrogel (NH2-DN) with excellent mechanical and adsorption properties was successfully prepared.					
31683441	8	62	theme	kinetic	1237:1243	arg1	analyses					1245:1252	Adsorption isotherm and kinetic analyses	1213:1252	analyses	1245:1252	Adsorption isotherm and kinetic analyses reveal that the adsorption process of CIP onto the NH2-DN was dominated by chemical affinity.					
31683441	11	63	theme	novel	1661:1665	arg1	hydrogel					1706:1713	This novel amino-functionalized organic-inorganic hydrogel	1656:1713	This novel amino-functionalized organic-inorganic hydrogel	1656:1713	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	1	64	theme	recent	196:201	arg1	years					203:207	recent years	196:207	recent years	196:207	Inorganic-organic composite hydrogels have attracted much attention in recent years.					
31683441	5	65	theme	double-network	820:833	arg1	formation					803:811	the formation	799:811	the formation of the double-network	799:833	The elasticity modulus was improved by approximately 3 times due to the formation of the double-network.					
31683441	6	66	theme	emerging	915:922	arg1	ciprofloxacin					947:959	ciprofloxacin	947:959	ciprofloxacin	947:959	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	6	66	theme	emerging	915:922	arg1	Cu2+					938:941	Cu2+	938:941	Cu2+	938:941	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	6	66	theme	emerging	915:922	arg1	contaminants					924:935	typical emerging contaminants	907:935	typical emerging contaminants (Cu2+ and ciprofloxacin (CIP))	907:966	More importantly, NH2-DN exhibited excellent adsorption properties for typical emerging contaminants (Cu2+ and ciprofloxacin (CIP)).					
31683441	11	67	theme	amino-functionalized	1667:1686	arg1	hydrogel					1706:1713	This novel amino-functionalized organic-inorganic hydrogel	1656:1713	This novel amino-functionalized organic-inorganic hydrogel	1656:1713	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	10	68	theme	capacity	1608:1615	arg1	capacity					1608:1615	its adsorption capacity	1593:1615	its adsorption capacity	1593:1615	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	10	68	theme	capacity	1608:1615	arg1	%					1588:1588	at least 87%	1577:1588	at least 87% of its adsorption capacity	1577:1615	NH2-DN retained 94% of its adsorption capacity when the ionic strength was 0.5 mol L-1 and maintained at least 87% of its adsorption capacity in weak acidic and alkaline solutions.					
31683441	8	69	theme	CIP	1292:1294	arg1	process					1281:1287	the adsorption process	1266:1287	the adsorption process of CIP onto the NH2-DN	1266:1310	Adsorption isotherm and kinetic analyses reveal that the adsorption process of CIP onto the NH2-DN was dominated by chemical affinity.					
31683441	11	70	theme	emerging	1849:1856	arg1	contaminants					1858:1869	emerging contaminants	1849:1869	emerging contaminants in wastewater	1849:1883	This novel amino-functionalized organic-inorganic hydrogel has great potential in environmental applications owing to its outstanding physicochemical, mechanical, and adsorption properties for emerging contaminants in wastewater.					
31683441	3	71	theme	random	448:453	arg1	sheets					474:479	random few-layer graphene sheets	448:479	random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface	448:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	3	72	theme	few-layer	455:463	arg1	sheets					474:479	random few-layer graphene sheets	448:479	random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface	448:613	Triethylenetetramine (TETA) was used as a crosslinker which promotes random few-layer graphene sheets stacking and resulted in a reduced graphene oxide (rGO) network, containing mesopore and macropore structures on the hydrogel surface.					
31683441	4	73	theme	mechanical	684:693	arg1	properties					695:704	mechanical properties	684:704	mechanical properties	684:704	Compared to single network hydrogel, enhanced thermal stability and mechanical properties were achieved in NH2-DN.					
31079331	4	0	theme	pairwise	779:786	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	9	1	dep	characteristics	1676:1690	arg1	basis					1611:1615	basis	1611:1615	basis	1611:1615	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	9	1	dep	characteristics	1676:1690	arg1	the					1607:1609	the	1607:1609	the	1607:1609	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	9	2	theme	dokdonellae	1793:1803	arg1	sp					1805:1806	the name Arthrobacter dokdonellae sp	1771:1806	the name Arthrobacter dokdonellae sp	1771:1806	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	2	3	theme	aerobic	143:149	arg1	strain					177:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	3	4	theme	NaCl	431:434	arg1	concentration					436:448	0-5% (w/v) NaCl concentration	420:448	0-5% (w/v) NaCl concentration (optimum 0%)	420:461	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	4	5	theme	Arthrobacter	735:746	arg1	GP3T					769:772	Arthrobacter psychrochitiniphilus GP3T	735:772	Arthrobacter psychrochitiniphilus GP3T	735:772	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	7	6	theme	unidentified	1462:1473	arg1	GL					1487:1488	GL	1487:1488	GL	1487:1488	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), unidentified glycolipid (GL), two unidentified aminophospholipids (APLs), and three unidentified lipids (Ls).					
31079331	7	6	theme	unidentified	1462:1473	arg1	glycolipid					1475:1484	unidentified glycolipid	1462:1484	unidentified glycolipid (GL)	1462:1489	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), unidentified glycolipid (GL), two unidentified aminophospholipids (APLs), and three unidentified lipids (Ls).					
31079331	2	7	theme	motile	135:140	arg1	strain					177:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	6	8	theme	major	1246:1250	arg1	MK-9					1264:1267	MK-9	1264:1267	MK-9(H2)	1264:1271	The major quinone was MK-9(H2) and major fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C15:0, and iso-C16:0.					
31079331	6	8	theme	major	1246:1250	arg1	quinone					1252:1258	The major quinone	1242:1258	The major quinone	1242:1258	The major quinone was MK-9(H2) and major fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C15:0, and iso-C16:0.					
31079331	5	9	theme	34.73	1179:1183	arg1	%					1191:1191	34.73 ± 1.86%	1179:1191	34.73 ± 1.86%	1179:1191	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	2	10	theme	Dokdo	276:280	arg1	Republic					290:297	Republic	290:297	Republic of Korea	290:306	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	2	10	theme	Dokdo	276:280	arg1	island					282:287	Dokdo island	276:287	Dokdo island	276:287	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	4	11	theme	%	826:826	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	12	dep	A.	1081:1082	arg1	ramosus					1084:1090	ramosus	1084:1090	ramosus	1084:1090	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	4	13	dep	Arthrobacter	592:603	arg1	livingstonensis					605:619	livingstonensis	605:619	livingstonensis	605:619	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	4	14	with	2783T	659:663	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	15	theme	DCT-5T	909:914	arg1	%					928:928	64.7 mol%	920:928	64.7 mol%	920:928	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	15	theme	DCT-5T	909:914	arg1	content					891:897	The DNA G+C content	879:897	The DNA G+C content of strain DCT-5T	879:914	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	4	16	theme	strain	514:519	arg1	DCT-5T					521:526	strain DCT-5T	514:526	strain DCT-5T	514:526	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	17	theme	64.7	920:923	arg1	mol					925:927	mol	925:927	mol	925:927	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	18	dep	A.	1051:1052	arg1	GP3T					1075:1078	A. psychrochitiniphilus GP3T	1051:1078	A. psychrochitiniphilus GP3T	1051:1078	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	4	19	with	KIS14-16T	581:589	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	20	theme	G+C	887:889	arg1	%					928:928	64.7 mol%	920:928	64.7 mol%	920:928	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	20	theme	G+C	887:889	arg1	content					891:897	The DNA G+C content	879:897	The DNA G+C content of strain DCT-5T	879:914	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	6	21	theme	major	1277:1281	arg1	acids					1289:1293	major fatty acids	1277:1293	major fatty acids	1277:1293	The major quinone was MK-9(H2) and major fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C15:0, and iso-C16:0.					
31079331	2	22	theme	native	227:232	arg1	plant					234:238	a native plant	225:238	a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea	225:306	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	5	23	theme	DNA-DNA	939:945	arg1	relatedness					947:957	its DNA-DNA relatedness	935:957	its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T	935:1127	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	4	24	with	GP3T	769:772	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	4	25	theme	silviterrae	569:579	arg1	KIS14-16T					581:589	Arthrobacter silviterrae KIS14-16T	556:589	Arthrobacter silviterrae KIS14-16T	556:589	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	26	theme	A.	1081:1082	arg1	KIS14-16T					986:994	A. silviterrae KIS14-16T	971:994	A. silviterrae KIS14-16T	971:994	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	26	theme	A.	1081:1082	arg1	1646T					1096:1100	A. ramosus CCM 1646T	1081:1100	A. ramosus CCM 1646T	1081:1100	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	2	27	from	island	282:287	arg1	Campanula					263:271	the genus Campanula	253:271	the genus Campanula at Dokdo island, Republic of Korea	253:306	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	2	28	attach	isolated	211:218	arg2	strain					177:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	2	28	attach	isolated	211:218	arg1	plant					234:238	a native plant	225:238	a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea	225:306	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	11	29	theme	=	1868:1868	arg1	31284T					1874:1879	= KCTC 49189T = LMG 31284T	1854:1879	= KCTC 49189T = LMG 31284T	1854:1879	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	11	29	theme	=	1868:1868	arg1	DCT-5T					1846:1851	DCT-5T	1846:1851	DCT-5T (= KCTC 49189T = LMG 31284T)	1846:1880	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	5	30	theme	±	1219:1219	arg1	%					1225:1225	27.23 ± 0.88%	1213:1225	27.23 ± 0.88%	1213:1225	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	31	theme	CCM	1092:1094	arg1	KIS14-16T					986:994	A. silviterrae KIS14-16T	971:994	A. silviterrae KIS14-16T	971:994	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	31	theme	CCM	1092:1094	arg1	1646T					1096:1100	A. ramosus CCM 1646T	1081:1100	A. ramosus CCM 1646T	1081:1100	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	4	32	theme	%	842:842	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	33	theme	±	1200:1200	arg1	%					1206:1206	29.12 ± 1.56%	1194:1206	29.12 ± 1.56%	1194:1206	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	9	34	theme	chemotaxonomic	1661:1674	arg1	characteristics					1676:1690	phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics	1620:1690	phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics	1620:1690	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	5	35	theme	2.02	1142:1145	arg1	±					1140:1140	±	1140:1140	±	1140:1140	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	0	36	theme	Arthrobacter	0:11	arg1	sp					25:26	Arthrobacter dokdonellae sp	0:26	Arthrobacter dokdonellae sp.	0:27	Arthrobacter dokdonellae sp.					
31079331	1	37	theme	Campanula	70:78	arg1	plant					51:55	a plant	49:55	a plant of the genus Campanula	49:78	nov., isolated from a plant of the genus Campanula.					
31079331	3	38	theme	strain	323:328	arg1	DCT-5T					330:335	the strain DCT-5T	319:335	the strain DCT-5T	319:335	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	4	39	theme	stackebrandtii	640:653	arg1	2783T					659:663	Arthrobacter stackebrandtii CCM 2783T	627:663	Arthrobacter stackebrandtii CCM 2783T	627:663	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	3	40	theme	pH	390:391	arg1	optimum					402:408	pH 6.0-8.0 (optimum 7.0)	390:413	pH 6.0-8.0 (optimum 7.0)	390:413	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	3	41	dep	%	423:423	arg1	w/v					426:428	w/v	426:428	w/v	426:428	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	11	42	theme	type	1831:1834	arg1	DCT-5T					1846:1851	DCT-5T	1846:1851	DCT-5T (= KCTC 49189T = LMG 31284T)	1846:1880	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	11	42	theme	type	1831:1834	arg1	strain					1836:1841	The type strain	1827:1841	The type strain	1827:1841	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	2	43	theme	rod-shaped	156:165	arg1	strain					177:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	4	44	theme	CCM	720:722	arg1	1646T					724:728	Arthrobacter ramosus CCM 1646T	699:728	Arthrobacter ramosus CCM 1646T	699:728	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	45	theme	32.57	1134:1138	arg1	±					1140:1140	±	1140:1140	±	1140:1140	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	46	theme	A.	971:972	arg1	A.					997:998	A.	997:998	A.	997:998	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	46	theme	A.	971:972	arg1	Cr6-08T					1121:1127	Cr6-08T	1121:1127	Cr6-08T	1121:1127	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	46	theme	A.	971:972	arg1	A.					1051:1052	A.	1051:1052	A.	1051:1052	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	46	theme	A.	971:972	arg1	1646T					1096:1100	A. ramosus CCM 1646T	1081:1100	A. ramosus CCM 1646T	1081:1100	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	46	theme	A.	971:972	arg1	KIS14-16T					986:994	A. silviterrae KIS14-16T	971:994	A. silviterrae KIS14-16T	971:994	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	46	theme	A.	971:972	arg1	A.					1022:1023	A.	1022:1023	A.	1022:1023	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	47	theme	31.93	1164:1168	arg1	%					1176:1176	31.93 ± 1.15%	1164:1176	31.93 ± 1.15%	1164:1176	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	2	48	theme	Korea	302:306	arg1	Republic					290:297	Republic	290:297	Republic of Korea	290:306	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	2	48	theme	Korea	302:306	arg1	island					282:287	Dokdo island	276:287	Dokdo island	276:287	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	3	49	located	observed	341:348	arg1	30°C					370:373	optimum 30°C	362:373	optimum 30°C	362:373	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	3	49	located	observed	341:348	arg1	concentration					436:448	0-5% (w/v) NaCl concentration	420:448	0-5% (w/v) NaCl concentration (optimum 0%)	420:461	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	3	49	located	observed	341:348	arg1	optimum					402:408	pH 6.0-8.0 (optimum 7.0)	390:413	pH 6.0-8.0 (optimum 7.0)	390:413	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	3	49	located	observed	341:348	arg1	15-37°C					353:359	15-37°C	353:359	15-37°C (optimum 30°C)	353:374	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	3	49	located	observed	341:348	arg2	Growth					309:314	Growth	309:314	Growth of the strain DCT-5T	309:335	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	3	49	located	observed	341:348	arg1	broth					383:387	R2A broth	379:387	R2A broth	379:387	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	5	50	theme	DNA	883:885	arg1	%					928:928	64.7 mol%	920:928	64.7 mol%	920:928	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	50	theme	DNA	883:885	arg1	content					891:897	The DNA G+C content	879:897	The DNA G+C content of strain DCT-5T	879:914	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	7	51	theme	unidentified	1496:1507	arg1	aminophospholipids					1509:1526	two unidentified aminophospholipids	1492:1526	two unidentified aminophospholipids (APLs)	1492:1533	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), unidentified glycolipid (GL), two unidentified aminophospholipids (APLs), and three unidentified lipids (Ls).					
31079331	7	51	theme	unidentified	1496:1507	arg1	APLs					1529:1532	APLs	1529:1532	APLs	1529:1532	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), unidentified glycolipid (GL), two unidentified aminophospholipids (APLs), and three unidentified lipids (Ls).					
31079331	4	52	theme	16S	468:470	arg1	rRNA					472:475	The 16S rRNA	464:475	The 16S rRNA gene sequence analysis	464:498	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	9	53	theme	Arthrobacter	1747:1758	arg1	species					1726:1732	a novel species	1718:1732	a novel species	1718:1732	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	4	54	theme	Arthrobacter	699:710	arg1	1646T					724:728	Arthrobacter ramosus CCM 1646T	699:728	Arthrobacter ramosus CCM 1646T	699:728	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	55	theme	±	1155:1155	arg1	%					1161:1161	28.75 ± 0.88%	1149:1161	28.75 ± 0.88%	1149:1161	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	3	56	theme	0-5	420:422	arg1	%					423:423	%	423:423	%	423:423	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	4	57	theme	gene	477:480	arg1	analysis					491:498	The 16S rRNA gene sequence analysis	464:498	The 16S rRNA gene sequence analysis	464:498	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	2	58	theme	oxidase-	104:111	arg1	strain					177:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	2	59	theme	Gram-stain-positive	83:101	arg1	strain					177:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	2	60	theme	genus	257:261	arg1	Campanula					263:271	the genus Campanula	253:271	the genus Campanula at Dokdo island, Republic of Korea	253:306	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	5	61	theme	CCM	1040:1042	arg1	2783T					1044:1048	CCM 2783T	1040:1048	A. stackebrandtii CCM 2783T	1022:1048	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	4	62	theme	sequence	788:795	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	9	63	theme	Arthrobacter	1780:1791	arg1	sp					1805:1806	the name Arthrobacter dokdonellae sp	1771:1806	the name Arthrobacter dokdonellae sp	1771:1806	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	5	64	theme	29.12	1194:1198	arg1	%					1206:1206	29.12 ± 1.56%	1194:1206	29.12 ± 1.56%	1194:1206	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	4	65	dep	Arthrobacter	699:710	arg1	ramosus					712:718	ramosus	712:718	ramosus	712:718	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	2	66	theme	catalase-positive	117:133	arg1	strain					177:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	3	67	theme	optimum	451:457	arg1	concentration					436:448	0-5% (w/v) NaCl concentration	420:448	0-5% (w/v) NaCl concentration (optimum 0%)	420:461	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	5	68	theme	±	1185:1185	arg1	%					1191:1191	34.73 ± 1.86%	1179:1191	34.73 ± 1.86%	1179:1191	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	8	69	theme	peptidoglycan	1576:1588	arg1	A3α					1599:1601	A3α	1599:1601	A3α	1599:1601	The peptidoglycan type was A3α.					
31079331	8	69	theme	peptidoglycan	1576:1588	arg1	type					1590:1593	The peptidoglycan type	1572:1593	The peptidoglycan type	1572:1593	The peptidoglycan type was A3α.					
31079331	5	70	theme	mol	925:927	arg1	%					928:928	64.7 mol%	920:928	64.7 mol%	920:928	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	70	theme	mol	925:927	arg1	content					891:897	The DNA G+C content	879:897	The DNA G+C content of strain DCT-5T	879:914	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	6	71	theme	fatty	1283:1287	arg1	acids					1289:1293	major fatty acids	1277:1293	major fatty acids	1277:1293	The major quinone was MK-9(H2) and major fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C15:0, and iso-C16:0.					
31079331	4	72	with	LI2T	621:624	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	4	73	theme	%	818:818	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	74	dep	A.	1022:1023	arg1	2783T					1044:1048	CCM 2783T	1040:1048	A. stackebrandtii CCM 2783T	1022:1048	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	74	dep	A.	1022:1023	arg1	stackebrandtii					1025:1038	A. stackebrandtii CCM 2783T	1022:1048	A. stackebrandtii CCM 2783T	1022:1048	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	9	75	theme	phenotypic	1620:1629	arg1	characteristics					1676:1690	phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics	1620:1690	phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics	1620:1690	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	4	76	theme	Arthrobacter	592:603	arg1	LI2T					621:624	Arthrobacter livingstonensis LI2T	592:624	Arthrobacter livingstonensis LI2T	592:624	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	4	77	with	1646T	724:728	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	78	theme	strain	902:907	arg1	DCT-5T					909:914	strain DCT-5T	902:914	strain DCT-5T	902:914	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	11	79	theme	=	1854:1854	arg1	31284T					1874:1879	= KCTC 49189T = LMG 31284T	1854:1879	= KCTC 49189T = LMG 31284T	1854:1879	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	11	79	theme	=	1854:1854	arg1	DCT-5T					1846:1851	DCT-5T	1846:1851	DCT-5T (= KCTC 49189T = LMG 31284T)	1846:1880	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	9	80	theme	phylogenetic	1632:1643	arg1	characteristics					1676:1690	phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics	1620:1690	phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics	1620:1690	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	5	81	with	values	959:964	arg1	A.					997:998	A.	997:998	A.	997:998	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	81	with	values	959:964	arg1	Cr6-08T					1121:1127	Cr6-08T	1121:1127	Cr6-08T	1121:1127	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	81	with	values	959:964	arg1	A.					1051:1052	A.	1051:1052	A.	1051:1052	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	81	with	values	959:964	arg1	1646T					1096:1100	A. ramosus CCM 1646T	1081:1100	A. ramosus CCM 1646T	1081:1100	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	81	with	values	959:964	arg1	KIS14-16T					986:994	A. silviterrae KIS14-16T	971:994	A. silviterrae KIS14-16T	971:994	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	81	with	values	959:964	arg1	A.					1022:1023	A.	1022:1023	A.	1022:1023	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	11	82	theme	LMG	1870:1872	arg1	31284T					1874:1879	= KCTC 49189T = LMG 31284T	1854:1879	= KCTC 49189T = LMG 31284T	1854:1879	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	11	82	theme	LMG	1870:1872	arg1	DCT-5T					1846:1851	DCT-5T	1846:1851	DCT-5T (= KCTC 49189T = LMG 31284T)	1846:1880	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	5	83	theme	relatedness	947:957	arg1	values					959:964	its DNA-DNA relatedness values	935:964	its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T	935:1127	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	3	84	dep	optimum	451:457	arg1	%					460:460	0%	459:460	0%	459:460	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	4	85	theme	%	850:850	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	86	dep	A.	997:998	arg1	livingstonensis					1000:1014	A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T	997:1127	livingstonensis	1000:1014	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	86	dep	A.	997:998	arg1	LI2T					1016:1019	LI2T	1016:1019	LI2T	1016:1019	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	5	87	theme	27.23	1213:1217	arg1	%					1225:1225	27.23 ± 0.88%	1213:1225	27.23 ± 0.88%	1213:1225	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	9	88	theme	genotypic	1646:1654	arg1	characteristics					1676:1690	phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics	1620:1690	phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics	1620:1690	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	4	89	theme	Arthrobacter	556:567	arg1	KIS14-16T					581:589	Arthrobacter silviterrae KIS14-16T	556:589	Arthrobacter silviterrae KIS14-16T	556:589	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	4	90	theme	%	834:834	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	4	91	theme	CCM	655:657	arg1	2783T					659:663	Arthrobacter stackebrandtii CCM 2783T	627:663	Arthrobacter stackebrandtii CCM 2783T	627:663	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	11	92	theme	49189T	1861:1866	arg1	31284T					1874:1879	= KCTC 49189T = LMG 31284T	1854:1879	= KCTC 49189T = LMG 31284T	1854:1879	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	11	92	theme	49189T	1861:1866	arg1	DCT-5T					1846:1851	DCT-5T	1846:1851	DCT-5T (= KCTC 49189T = LMG 31284T)	1846:1880	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	5	93	dep	A.	971:972	arg1	silviterrae					974:984	silviterrae	974:984	silviterrae	974:984	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	3	94	theme	R2A	379:381	arg1	broth					383:387	R2A broth	379:387	R2A broth	379:387	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	0	95	theme	dokdonellae	13:23	arg1	sp					25:26	Arthrobacter dokdonellae sp	0:26	Arthrobacter dokdonellae sp.	0:27	Arthrobacter dokdonellae sp.					
31079331	1	96	theme	genus	64:68	arg1	Campanula					70:78	the genus Campanula	60:78	the genus Campanula	60:78	nov., isolated from a plant of the genus Campanula.					
31079331	9	97	theme	strain	1693:1698	arg1	DCT-5T					1700:1705	strain DCT-5T	1693:1705	strain DCT-5T	1693:1705	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	4	98	dep	Arthrobacter	735:746	arg1	psychrochitiniphilus					748:767	psychrochitiniphilus	748:767	psychrochitiniphilus	748:767	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	7	99	theme	polar	1360:1364	arg1	diphosphatidylglycerol					1378:1399	diphosphatidylglycerol	1378:1399	diphosphatidylglycerol (DPG)	1378:1405	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), unidentified glycolipid (GL), two unidentified aminophospholipids (APLs), and three unidentified lipids (Ls).					
31079331	7	99	theme	polar	1360:1364	arg1	lipids					1366:1371	The polar lipids	1356:1371	The polar lipids	1356:1371	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), unidentified glycolipid (GL), two unidentified aminophospholipids (APLs), and three unidentified lipids (Ls).					
31079331	3	100	theme	DCT-5T	330:335	arg1	Growth					309:314	Growth	309:314	Growth of the strain DCT-5T	309:335	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	2	101	theme	bacterial	167:175	arg1	strain					177:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain	81:182	A Gram-stain-positive, oxidase- and catalase-positive motile, aerobic, and rod-shaped bacterial strain, designated as DCT-5T, was isolated from a native plant belonging to the genus Campanula at Dokdo island, Republic of Korea.					
31079331	4	102	theme	Arthrobacter	627:638	arg1	2783T					659:663	Arthrobacter stackebrandtii CCM 2783T	627:663	Arthrobacter stackebrandtii CCM 2783T	627:663	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	7	103	theme	unidentified	1546:1557	arg1	lipids					1559:1564	three unidentified lipids	1540:1564	three unidentified lipids (Ls)	1540:1569	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), unidentified glycolipid (GL), two unidentified aminophospholipids (APLs), and three unidentified lipids (Ls).					
31079331	7	103	theme	unidentified	1546:1557	arg1	Ls					1567:1568	Ls	1567:1568	Ls	1567:1568	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI), unidentified glycolipid (GL), two unidentified aminophospholipids (APLs), and three unidentified lipids (Ls).					
31079331	3	104	theme	optimum	362:368	arg1	15-37°C					353:359	15-37°C	353:359	15-37°C (optimum 30°C)	353:374	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	3	104	theme	optimum	362:368	arg1	30°C					370:373	optimum 30°C	362:373	optimum 30°C	362:373	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31079331	9	105	theme	novel	1720:1724	arg1	species					1726:1732	a novel species	1718:1732	a novel species	1718:1732	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	4	106	theme	%	862:862	arg1	similarities					797:808	pairwise sequence similarities	779:808	pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively	779:876	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	5	107	theme	±	1170:1170	arg1	%					1176:1176	31.93 ± 1.15%	1164:1176	31.93 ± 1.15%	1164:1176	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	11	108	theme	KCTC	1856:1859	arg1	31284T					1874:1879	= KCTC 49189T = LMG 31284T	1854:1879	= KCTC 49189T = LMG 31284T	1854:1879	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	11	108	theme	KCTC	1856:1859	arg1	DCT-5T					1846:1851	DCT-5T	1846:1851	DCT-5T (= KCTC 49189T = LMG 31284T)	1846:1880	The type strain is DCT-5T (= KCTC 49189T = LMG 31284T).					
31079331	4	109	theme	rRNA	472:475	arg1	analysis					491:498	The 16S rRNA gene sequence analysis	464:498	The 16S rRNA gene sequence analysis	464:498	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	9	110	theme	genus	1741:1745	arg1	Arthrobacter					1747:1758	the genus Arthrobacter	1737:1758	the genus Arthrobacter	1737:1758	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	5	111	theme	28.75	1149:1153	arg1	%					1161:1161	28.75 ± 0.88%	1149:1161	28.75 ± 0.88%	1149:1161	The DNA G+C content of strain DCT-5T was 64.7 mol%, and its DNA-DNA relatedness values with A. silviterrae KIS14-16T, A. livingstonensis LI2T, A. stackebrandtii CCM 2783T, A. psychrochitiniphilus GP3T, A. ramosus CCM 1646T, and A. cryoconiti Cr6-08T were 32.57 ± 2.02%, 28.75 ± 0.88%, 31.93 ± 1.15%, 34.73 ± 1.86%, 29.12 ± 1.56%, and 27.23 ± 0.88%, respectively.					
31079331	9	112	theme	name	1775:1778	arg1	sp					1805:1806	the name Arthrobacter dokdonellae sp	1771:1806	the name Arthrobacter dokdonellae sp	1771:1806	On the basis of phenotypic, phylogenetic, genotypic, and chemotaxonomic characteristics, strain DCT-5T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter dokdonellae sp.					
31079331	4	113	theme	sequence	482:489	arg1	analysis					491:498	The 16S rRNA gene sequence analysis	464:498	The 16S rRNA gene sequence analysis	464:498	The 16S rRNA gene sequence analysis revealed that strain DCT-5T was most closely related to Arthrobacter silviterrae KIS14-16T, Arthrobacter livingstonensis LI2T, Arthrobacter stackebrandtii CCM 2783T, Arthrobacter cryoconiti Cr6-08T, Arthrobacter ramosus CCM 1646T, and Arthrobacter psychrochitiniphilus GP3T with pairwise sequence similarities of 98.76%, 97.47%, 97.25%, 97.11%, 97.11%, and 97.00%, respectively.					
31079331	3	114	theme	%	423:423	arg1	concentration					436:448	0-5% (w/v) NaCl concentration	420:448	0-5% (w/v) NaCl concentration (optimum 0%)	420:461	Growth of the strain DCT-5T was observed at 15-37°C (optimum 30°C) on R2A broth, pH 6.0-8.0 (optimum 7.0), and 0-5% (w/v) NaCl concentration (optimum 0%).					
31195972	12	0	from	SCFAs	2002:2006	arg1	mice					2024:2027	mice	2024:2027	mice	2024:2027	CONCLUSION We observed a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose.					
31195972	6	1	theme	mice	1057:1060	arg1	feces					1048:1052	the feces	1044:1052	the feces of mice treated with acarbose	1044:1082	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	9	2	theme	acetate	1522:1528	arg1	concentrations					1504:1517	the concentrations	1500:1517	the concentrations of acetate and butyrate	1500:1541	Propionate concentrations in feces were consistently elevated in treated mice, while the concentrations of acetate and butyrate reflected a dependence on study site.					
31195972	10	3	theme	concentrations	1727:1740	arg1	composition					1680:1690	community composition	1670:1690	community composition	1670:1690	Across all samples, Muribaculaceae abundance was strongly correlated with propionate and community composition was an important predictor of SCFA concentrations.					
31195972	10	3	theme	concentrations	1727:1740	arg1	predictor					1709:1717	an important predictor	1696:1717	an important predictor of SCFA concentrations	1696:1740	Across all samples, Muribaculaceae abundance was strongly correlated with propionate and community composition was an important predictor of SCFA concentrations.					
31195972	13	4	theme	responses	2249:2257	arg1	site-dependence					2226:2240	the site-dependence	2222:2240	the site-dependence of the responses	2222:2257	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	12	5	theme	longevity-enhancing	2077:2095	arg1	properties					2097:2106	the longevity-enhancing properties	2073:2106	the longevity-enhancing properties of acarbose	2073:2118	CONCLUSION We observed a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose.					
31195972	14	6	theme	future	2373:2378	arg1	studies					2380:2386	future studies	2373:2386	future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose	2373:2561	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	2	7	theme	longevity	264:272	arg1	extension					274:282	This longevity extension	259:282	This longevity extension	259:282	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	13	8	theme	microbiome	2330:2339	arg1	studies					2341:2347	microbiome studies	2330:2347	microbiome studies	2330:2347	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	6	9	theme	control	1121:1127	arg1	mice					1129:1132	control mice	1121:1132	control mice	1121:1132	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	4	10	theme	short-chain	660:670	arg1	products					706:713	the dominant products	693:713	the dominant products of starch fermentation by gut bacteria	693:752	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	4	10	theme	short-chain	660:670	arg1	SCFAs					685:689	SCFAs	685:689	SCFAs	685:689	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	4	10	theme	short-chain	660:670	arg1	acids					678:682	short-chain fatty acids	660:682	short-chain fatty acids (SCFAs)	660:690	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	8	11	from	two	1391:1393	arg1	unit					1383:1386	the same taxonomic unit	1364:1386	the same taxonomic unit	1364:1386	The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites.					
31195972	12	12	theme	gut	2055:2057	arg1	microbiota					2059:2068	the gut microbiota	2051:2068	the gut microbiota	2051:2068	CONCLUSION We observed a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose.					
31195972	2	13	theme	dietary	297:303	arg1	restriction					305:315	dietary restriction	297:315	dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect	297:442	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	9	14	theme	Propionate	1415:1424	arg1	concentrations					1426:1439	Propionate concentrations	1415:1439	Propionate concentrations in feces	1415:1448	Propionate concentrations in feces were consistently elevated in treated mice, while the concentrations of acetate and butyrate reflected a dependence on study site.					
31195972	4	15	theme	documented	630:639	arg1	benefits					648:655	the documented health benefits	626:655	the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria	626:752	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	11	16	theme	acetate	1819:1825	arg1	predictive					1869:1878	predictive	1869:1878	predictive	1869:1878	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	11	16	theme	acetate	1819:1825	arg1	concentrations					1801:1814	the fecal concentrations	1791:1814	the fecal concentrations of acetate, butyrate, and propionate	1791:1851	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	1	17	theme	male	230:233	arg1	mice					235:238	male mice	230:238	male mice	230:238	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	1	18	from	%	245:245	arg1	females					250:256	females	250:256	females	250:256	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	1	18	from	%	245:245	arg1	mice					235:238	male mice	230:238	male mice	230:238	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	5	19	theme	fecal	882:886	arg1	microbiome					888:897	the fecal microbiome	878:897	the fecal microbiome of mice treated with acarbose to control mice	878:943	To explore this hypothesis, we compared the fecal microbiome of mice treated with acarbose to control mice at three independent study sites.					
31195972	4	20	theme	dominant	697:704	arg1	products					706:713	the dominant products	693:713	the dominant products of starch fermentation by gut bacteria	693:752	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	4	20	theme	dominant	697:704	arg1	acids					678:682	short-chain fatty acids	660:682	short-chain fatty acids (SCFAs)	660:690	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	6	21	theme	Microbial	987:995	arg1	communities					997:1007	Microbial communities	987:1007	Microbial communities	987:1007	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	8	22	theme	blooming	1256:1263	arg1	populations					1265:1275	The blooming populations	1252:1275	The blooming populations	1252:1275	The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites.					
31195972	0	23	theme	concurrent	56:65	arg1	products					47:54	and fermentation products	30:54	products	47:54	Changes in the gut microbiome and fermentation products concurrent with enhanced longevity in acarbose-treated mice.					
31195972	9	24	theme	treated	1480:1486	arg1	mice					1488:1491	treated mice	1480:1491	treated mice	1480:1491	Propionate concentrations in feces were consistently elevated in treated mice, while the concentrations of acetate and butyrate reflected a dependence on study site.					
31195972	11	25	theme	hazards	1760:1766	arg1	regression					1768:1777	Cox proportional hazards regression	1743:1777	Cox proportional hazards regression	1743:1777	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	0	26	theme	enhanced	72:79	arg1	longevity					81:89	enhanced longevity	72:89	enhanced longevity in acarbose-treated mice	72:114	Changes in the gut microbiome and fermentation products concurrent with enhanced longevity in acarbose-treated mice.					
31195972	0	27	from	Changes	0:6	arg1	gut					15:17	the gut	11:17	the gut	11:17	Changes in the gut microbiome and fermentation products concurrent with enhanced longevity in acarbose-treated mice.					
31195972	6	28	from	communities	997:1007	arg1	feces					1048:1052	the feces	1044:1052	the feces of mice treated with acarbose	1044:1082	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	4	29	theme	gut	741:743	arg1	bacteria					745:752	gut bacteria	741:752	gut bacteria	741:752	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	4	30	theme	starch	718:723	arg1	fermentation					725:736	starch fermentation	718:736	starch fermentation	718:736	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	11	31	theme	Cox	1743:1745	arg1	regression					1768:1777	Cox proportional hazards regression	1743:1777	Cox proportional hazards regression	1743:1777	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	5	32	theme	control	932:938	arg1	mice					940:943	control mice	932:943	control mice	932:943	To explore this hypothesis, we compared the fecal microbiome of mice treated with acarbose to control mice at three independent study sites.					
31195972	12	33	dep	CONCLUSION	1951:1960	arg1	observed					1965:1972	observed	1965:1972	observed	1965:1972	CONCLUSION We observed a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose.					
31195972	11	34	theme	mouse	1883:1887	arg1	longevity					1889:1897	mouse longevity	1883:1897	mouse longevity	1883:1897	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	7	35	theme	study	1148:1152	arg1	sites					1154:1158	all three study sites	1138:1158	all three study sites	1138:1158	At all three study sites, the bloom of a single bacterial taxon was the most obvious response to acarbose treatment.					
31195972	4	36	from	longevity	819:827	arg1	mice					832:835	mice	832:835	mice	832:835	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	8	37	theme	Bacteroidales	1319:1331	arg1	Muribaculaceae					1340:1353	the largely uncultured Bacteroidales family Muribaculaceae	1296:1353	the largely uncultured Bacteroidales family Muribaculaceae	1296:1353	The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites.					
31195972	7	38	from	sites	1154:1158	arg1	bloom					1165:1169	bloom	1165:1169	bloom	1165:1169	At all three study sites, the bloom of a single bacterial taxon was the most obvious response to acarbose treatment.					
31195972	7	38	from	sites	1154:1158	arg1	response					1220:1227	the most obvious response	1203:1227	the most obvious response to acarbose treatment	1203:1249	At all three study sites, the bloom of a single bacterial taxon was the most obvious response to acarbose treatment.					
31195972	13	39	theme	gut	2200:2202	arg1	microbiome					2204:2213	the gut microbiome	2196:2213	the gut microbiome	2196:2213	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	1	40	with	Treatment	128:136	arg1	acarbose					171:178	the α-glucosidase inhibitor acarbose	143:178	the α-glucosidase inhibitor acarbose	143:178	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	6	41	theme	SCFAs	1035:1039	arg1	concentrations					1017:1030	the concentrations	1013:1030	the concentrations of SCFAs in the feces of mice treated with acarbose	1013:1082	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	6	41	theme	SCFAs	1035:1039	arg1	different					1097:1105	different	1097:1105	different	1097:1105	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	6	41	theme	SCFAs	1035:1039	arg1	communities					997:1007	Microbial communities	987:1007	Microbial communities	987:1007	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	2	42	theme	lifespan	428:435	arg1	effect					437:442	the lifespan effect	424:442	the lifespan effect	424:442	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	4	43	theme	fermentation	725:736	arg1	products					706:713	the dominant products	693:713	the dominant products of starch fermentation by gut bacteria	693:752	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	4	43	theme	fermentation	725:736	arg1	acids					678:682	short-chain fatty acids	660:682	short-chain fatty acids (SCFAs)	660:690	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	3	44	theme	host	459:462	arg1	digestion					464:472	host digestion	459:472	host digestion	459:472	By inhibiting host digestion, acarbose increases the flux of starch to the lower digestive system, resulting in changes to the gut microbiota and their fermentation products.					
31195972	4	45	theme	acids	678:682	arg1	benefits					648:655	the documented health benefits	626:655	the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria	626:752	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	13	46	theme	fermentation	2171:2182	arg1	products					2184:2191	fermentation products	2171:2191	fermentation products	2171:2191	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	9	47	from	concentrations	1426:1439	arg1	feces					1444:1448	feces	1444:1448	feces	1444:1448	Propionate concentrations in feces were consistently elevated in treated mice, while the concentrations of acetate and butyrate reflected a dependence on study site.					
31195972	7	48	theme	acarbose	1232:1239	arg1	treatment					1241:1249	acarbose treatment	1232:1249	acarbose treatment	1232:1249	At all three study sites, the bloom of a single bacterial taxon was the most obvious response to acarbose treatment.					
31195972	8	49	theme	same	1368:1371	arg1	unit					1383:1386	the same taxonomic unit	1364:1386	the same taxonomic unit	1364:1386	The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites.					
31195972	14	50	theme	fermentation	2450:2461	arg1	products					2463:2470	its fermentation products	2446:2470	its fermentation products	2446:2470	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	2	51	theme	features	338:345	arg1	number					328:333	a number	326:333	a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect	326:442	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	2	51	theme	features	338:345	arg1	effects					379:385	the relatively small effects	358:385	the relatively small effects on weight and the sex-specificity of the lifespan effect	358:442	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	0	52	theme	fermentation	34:45	arg1	products					47:54	and fermentation products	30:54	products	47:54	Changes in the gut microbiome and fermentation products concurrent with enhanced longevity in acarbose-treated mice.					
31195972	14	53	theme	observed	2534:2541	arg1	effects					2543:2549	the observed effects	2530:2549	the observed effects of acarbose	2530:2561	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	9	54	theme	study	1569:1573	arg1	site					1575:1578	study site	1569:1578	study site	1569:1578	Propionate concentrations in feces were consistently elevated in treated mice, while the concentrations of acetate and butyrate reflected a dependence on study site.					
31195972	4	55	theme	secondary	760:768	arg1	effect					770:775	this secondary effect	755:775	this secondary effect of acarbose	755:787	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	11	56	theme	butyrate	1828:1835	arg1	predictive					1869:1878	predictive	1869:1878	predictive	1869:1878	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	11	56	theme	butyrate	1828:1835	arg1	concentrations					1801:1814	the fecal concentrations	1791:1814	the fecal concentrations of acetate, butyrate, and propionate	1791:1851	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	3	57	theme	lower	520:524	arg1	system					536:541	the lower digestive system	516:541	the lower digestive system	516:541	By inhibiting host digestion, acarbose increases the flux of starch to the lower digestive system, resulting in changes to the gut microbiota and their fermentation products.					
31195972	5	58	theme	independent	954:964	arg1	sites					972:976	three independent study sites	948:976	three independent study sites	948:976	To explore this hypothesis, we compared the fecal microbiome of mice treated with acarbose to control mice at three independent study sites.					
31195972	1	59	theme	inhibitor	161:169	arg1	acarbose					171:178	the α-glucosidase inhibitor acarbose	143:178	the α-glucosidase inhibitor acarbose	143:178	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	14	60	theme	microbial	2422:2430	arg1	community					2432:2440	the gut microbial community	2414:2440	the gut microbial community	2414:2440	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	3	61	theme	starch	506:511	arg1	flux					498:501	the flux	494:501	the flux of starch to the lower digestive system	494:541	By inhibiting host digestion, acarbose increases the flux of starch to the lower digestive system, resulting in changes to the gut microbiota and their fermentation products.					
31195972	14	62	theme	causal	2505:2510	arg1	roles					2512:2516	causal roles	2505:2516	causal roles of SCFAs in the observed effects of acarbose	2505:2561	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	7	63	theme	bacterial	1183:1191	arg1	taxon					1193:1197	a single bacterial taxon	1174:1197	a single bacterial taxon	1174:1197	At all three study sites, the bloom of a single bacterial taxon was the most obvious response to acarbose treatment.					
31195972	3	64	theme	digestive	526:534	arg1	system					536:541	the lower digestive system	516:541	the lower digestive system	516:541	By inhibiting host digestion, acarbose increases the flux of starch to the lower digestive system, resulting in changes to the gut microbiota and their fermentation products.					
31195972	0	65	with	concurrent	56:65	arg1	longevity					81:89	enhanced longevity	72:89	enhanced longevity in acarbose-treated mice	72:114	Changes in the gut microbiome and fermentation products concurrent with enhanced longevity in acarbose-treated mice.					
31195972	10	66	theme	SCFA	1722:1725	arg1	concentrations					1727:1740	SCFA concentrations	1722:1740	SCFA concentrations	1722:1740	Across all samples, Muribaculaceae abundance was strongly correlated with propionate and community composition was an important predictor of SCFA concentrations.					
31195972	1	67	theme	median	190:195	arg1	lifespan					197:204	median lifespan	190:204	median lifespan	190:204	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	14	68	theme	increased	2476:2484	arg1	longevity					2486:2494	increased longevity	2476:2494	increased longevity	2476:2494	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	4	69	theme	increased	809:817	arg1	longevity					819:827	increased longevity	809:827	increased longevity in mice	809:835	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	4	70	theme	health	641:646	arg1	benefits					648:655	the documented health benefits	626:655	the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria	626:752	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	11	71	theme	fecal	1795:1799	arg1	predictive					1869:1878	predictive	1869:1878	predictive	1869:1878	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	11	71	theme	fecal	1795:1799	arg1	concentrations					1801:1814	the fecal concentrations	1791:1814	the fecal concentrations of acetate, butyrate, and propionate	1791:1851	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	6	72	from	concentrations	1017:1030	arg1	feces					1048:1052	the feces	1044:1052	the feces of mice treated with acarbose	1044:1082	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	0	73	from	longevity	81:89	arg1	mice					111:114	acarbose-treated mice	94:114	acarbose-treated mice	94:114	Changes in the gut microbiome and fermentation products concurrent with enhanced longevity in acarbose-treated mice.					
31195972	11	74	theme	propionate	1842:1851	arg1	predictive					1869:1878	predictive	1869:1878	predictive	1869:1878	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	11	74	theme	propionate	1842:1851	arg1	concentrations					1801:1814	the fecal concentrations	1791:1814	the fecal concentrations of acetate, butyrate, and propionate	1791:1851	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	2	75	from	effects	379:385	arg1	weight					390:395	weight	390:395	weight	390:395	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	2	75	from	effects	379:385	arg1	sex-specificity					405:419	the sex-specificity	401:419	the sex-specificity of the lifespan effect	401:442	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	3	76	theme	gut	572:574	arg1	microbiota					576:585	the gut microbiota	568:585	the gut microbiota	568:585	By inhibiting host digestion, acarbose increases the flux of starch to the lower digestive system, resulting in changes to the gut microbiota and their fermentation products.					
31195972	8	77	theme	sites	1408:1412	arg1	sites					1408:1412	the three sites	1398:1412	the three sites	1398:1412	The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites.					
31195972	8	77	theme	sites	1408:1412	arg1	two					1391:1393	two	1391:1393	two	1391:1393	The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites.					
31195972	12	78	theme	microbiota	2059:2068	arg1	role					2043:2046	a role	2041:2046	a role of the gut microbiota in the longevity-enhancing properties of acarbose	2041:2118	CONCLUSION We observed a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose.					
31195972	12	79	from	role	2043:2046	arg1	properties					2097:2106	the longevity-enhancing properties	2073:2106	the longevity-enhancing properties of acarbose	2073:2118	CONCLUSION We observed a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose.					
31195972	1	80	theme	BACKGROUND	117:126	arg1	Treatment					128:136	BACKGROUND Treatment	117:136	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose	117:178	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	3	81	theme	fermentation	597:608	arg1	products					610:617	their fermentation products	591:617	their fermentation products	591:617	By inhibiting host digestion, acarbose increases the flux of starch to the lower digestive system, resulting in changes to the gut microbiota and their fermentation products.					
31195972	1	82	from	%	225:225	arg1	females					250:256	females	250:256	females	250:256	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	1	82	from	%	225:225	arg1	mice					235:238	male mice	230:238	male mice	230:238	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	1	83	theme	α-glucosidase	147:159	arg1	acarbose					171:178	the α-glucosidase inhibitor acarbose	143:178	the α-glucosidase inhibitor acarbose	143:178	BACKGROUND Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females.					
31195972	4	84	theme	fatty	672:676	arg1	products					706:713	the dominant products	693:713	the dominant products of starch fermentation by gut bacteria	693:752	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	4	84	theme	fatty	672:676	arg1	SCFAs					685:689	SCFAs	685:689	SCFAs	685:689	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	4	84	theme	fatty	672:676	arg1	acids					678:682	short-chain fatty acids	660:682	short-chain fatty acids (SCFAs)	660:690	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	12	85	from	lifespan	2012:2019	arg1	mice					2024:2027	mice	2024:2027	mice	2024:2027	CONCLUSION We observed a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose.					
31195972	13	86	dep	composition	2155:2165	arg1	the					2141:2143	the	2141:2143	the	2141:2143	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	0	87	theme	acarbose-treated	94:109	arg1	mice					111:114	acarbose-treated mice	94:114	acarbose-treated mice	94:114	Changes in the gut microbiome and fermentation products concurrent with enhanced longevity in acarbose-treated mice.					
31195972	12	88	theme	acarbose	2111:2118	arg1	properties					2097:2106	the longevity-enhancing properties	2073:2106	the longevity-enhancing properties of acarbose	2073:2118	CONCLUSION We observed a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose.					
31195972	6	89	dep	RESULTS	979:985	arg1	concentrations					1017:1030	the concentrations	1013:1030	the concentrations of SCFAs in the feces of mice treated with acarbose	1013:1082	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	6	89	dep	RESULTS	979:985	arg1	different					1097:1105	different	1097:1105	different	1097:1105	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	14	90	from	roles	2512:2516	arg1	effects					2543:2549	the observed effects	2530:2549	the observed effects of acarbose	2530:2561	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	11	91	theme	proportional	1747:1758	arg1	regression					1768:1777	Cox proportional hazards regression	1743:1777	Cox proportional hazards regression	1743:1777	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	13	92	dep	illustrate	2259:2268	arg1	site-dependence					2226:2240	the site-dependence	2222:2240	the site-dependence of the responses	2222:2257	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	13	92	dep	illustrate	2259:2268	arg1	while					2216:2220	while	2216:2220	while	2216:2220	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	10	93	theme	Muribaculaceae	1601:1614	arg1	abundance					1616:1624	Muribaculaceae abundance	1601:1624	Muribaculaceae abundance	1601:1624	Across all samples, Muribaculaceae abundance was strongly correlated with propionate and community composition was an important predictor of SCFA concentrations.					
31195972	5	94	theme	mice	902:905	arg1	microbiome					888:897	the fecal microbiome	878:897	the fecal microbiome of mice treated with acarbose to control mice	878:943	To explore this hypothesis, we compared the fecal microbiome of mice treated with acarbose to control mice at three independent study sites.					
31195972	2	95	theme	effect	437:442	arg1	weight					390:395	weight	390:395	weight	390:395	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	2	95	theme	effect	437:442	arg1	sex-specificity					405:419	the sex-specificity	401:419	the sex-specificity of the lifespan effect	401:442	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	8	96	theme	uncultured	1308:1317	arg1	Muribaculaceae					1340:1353	the largely uncultured Bacteroidales family Muribaculaceae	1296:1353	the largely uncultured Bacteroidales family Muribaculaceae	1296:1353	The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites.					
31195972	6	97	from	those	1112:1116	arg1	concentrations					1017:1030	the concentrations	1013:1030	the concentrations of SCFAs in the feces of mice treated with acarbose	1013:1082	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	6	97	from	those	1112:1116	arg1	different					1097:1105	different	1097:1105	different	1097:1105	RESULTS Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice.					
31195972	2	98	theme	small	373:377	arg1	effects					379:385	the relatively small effects	358:385	the relatively small effects on weight and the sex-specificity of the lifespan effect	358:442	This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect.					
31195972	13	99	theme	microbiome	2204:2213	arg1	composition					2155:2165	taxonomic composition	2145:2165	taxonomic composition	2145:2165	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	13	99	theme	microbiome	2204:2213	arg1	products					2184:2191	fermentation products	2171:2191	fermentation products	2171:2191	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	9	100	theme	butyrate	1534:1541	arg1	concentrations					1504:1517	the concentrations	1500:1517	the concentrations of acetate and butyrate	1500:1541	Propionate concentrations in feces were consistently elevated in treated mice, while the concentrations of acetate and butyrate reflected a dependence on study site.					
31195972	8	101	theme	family	1333:1338	arg1	Muribaculaceae					1340:1353	the largely uncultured Bacteroidales family Muribaculaceae	1296:1353	the largely uncultured Bacteroidales family Muribaculaceae	1296:1353	The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites.					
31195972	14	102	theme	acarbose	2554:2561	arg1	effects					2543:2549	the observed effects	2530:2549	the observed effects of acarbose	2530:2561	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	9	103	from	dependence	1555:1564	arg1	site					1575:1578	study site	1569:1578	study site	1569:1578	Propionate concentrations in feces were consistently elevated in treated mice, while the concentrations of acetate and butyrate reflected a dependence on study site.					
31195972	4	104	theme	acarbose	780:787	arg1	effect					770:775	this secondary effect	755:775	this secondary effect of acarbose	755:787	Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice.					
31195972	7	105	theme	obvious	1212:1218	arg1	bloom					1165:1169	bloom	1165:1169	bloom	1165:1169	At all three study sites, the bloom of a single bacterial taxon was the most obvious response to acarbose treatment.					
31195972	7	105	theme	obvious	1212:1218	arg1	response					1220:1227	the most obvious response	1203:1227	the most obvious response to acarbose treatment	1203:1249	At all three study sites, the bloom of a single bacterial taxon was the most obvious response to acarbose treatment.					
31195972	10	106	theme	community	1670:1678	arg1	composition					1680:1690	community composition	1670:1690	community composition	1670:1690	Across all samples, Muribaculaceae abundance was strongly correlated with propionate and community composition was an important predictor of SCFA concentrations.					
31195972	10	106	theme	community	1670:1678	arg1	predictor					1709:1717	an important predictor	1696:1717	an important predictor of SCFA concentrations	1696:1740	Across all samples, Muribaculaceae abundance was strongly correlated with propionate and community composition was an important predictor of SCFA concentrations.					
31195972	8	107	theme	taxonomic	1373:1381	arg1	unit					1383:1386	the same taxonomic unit	1364:1386	the same taxonomic unit	1364:1386	The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites.					
31195972	14	108	theme	community	2432:2440	arg1	manipulation					2398:2409	manipulation	2398:2409	manipulation of the gut microbial community	2398:2440	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	14	108	theme	community	2432:2440	arg1	products					2463:2470	its fermentation products	2446:2470	its fermentation products	2446:2470	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	13	109	theme	taxonomic	2145:2153	arg1	composition					2155:2165	taxonomic composition	2145:2165	taxonomic composition	2145:2165	Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the responses illustrate the challenges facing reproducibility and interpretation in microbiome studies.					
31195972	12	110	theme	fecal	1996:2000	arg1	SCFAs					2002:2006	fecal SCFAs	1996:2006	fecal SCFAs	1996:2006	CONCLUSION We observed a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose.					
31195972	14	111	theme	SCFAs	2521:2525	arg1	roles					2512:2516	causal roles	2505:2516	causal roles of SCFAs in the observed effects of acarbose	2505:2561	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	5	112	theme	study	966:970	arg1	sites					972:976	three independent study sites	948:976	three independent study sites	948:976	To explore this hypothesis, we compared the fecal microbiome of mice treated with acarbose to control mice at three independent study sites.					
31195972	11	113	theme	longevity	1889:1897	arg1	predictive					1869:1878	predictive	1869:1878	predictive	1869:1878	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	11	113	theme	longevity	1889:1897	arg1	concentrations					1801:1814	the fecal concentrations	1791:1814	the fecal concentrations of acetate, butyrate, and propionate	1791:1851	Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.					
31195972	14	114	theme	gut	2418:2420	arg1	community					2432:2440	the gut microbial community	2414:2440	the gut microbial community	2414:2440	These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, testing causal roles of SCFAs in the observed effects of acarbose.					
31195972	10	115	theme	important	1699:1707	arg1	composition					1680:1690	community composition	1670:1690	community composition	1670:1690	Across all samples, Muribaculaceae abundance was strongly correlated with propionate and community composition was an important predictor of SCFA concentrations.					
31195972	10	115	theme	important	1699:1707	arg1	predictor					1709:1717	an important predictor	1696:1717	an important predictor of SCFA concentrations	1696:1740	Across all samples, Muribaculaceae abundance was strongly correlated with propionate and community composition was an important predictor of SCFA concentrations.					
31195972	7	116	theme	single	1176:1181	arg1	taxon					1193:1197	a single bacterial taxon	1174:1197	a single bacterial taxon	1174:1197	At all three study sites, the bloom of a single bacterial taxon was the most obvious response to acarbose treatment.					
29760423	1	0	theme	microbiota	153:162	arg1	composition					164:174	the gut microbiota composition	145:174	the gut microbiota composition	145:174	Disturbances in the gut microbiota composition are associated with chronic inflammatory diseases of the intestine and the liver.					
29760423	6	1	theme	TNBS-suppressed	1025:1039	arg1	expression					1064:1073	TNBS-suppressed tight junction protein expression	1025:1073	TNBS-suppressed tight junction protein expression	1025:1073	Second, LC27 and LC67 inhibited TNBS-induced NF-κB activation, reversed TNBS-suppressed tight junction protein expression, and restored Th17/Treg balance.					
29760423	5	2	theme	lipopolysaccharide	926:943	arg1	levels					945:950	Bacteroidetes ratio and fecal and blood lipopolysaccharide levels	886:950	levels	945:950	These probiotics restored TNBS-induced disturbance of gut microbiota, leading to the suppression of Proteobacteria to Bacteroidetes ratio and fecal and blood lipopolysaccharide levels.					
29760423	0	3	from	Amelioratation	13:26	arg1	Mice					59:62	Mice	59:62	Mice	59:62	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.					
29760423	7	4	theme	TNBS-induced	1166:1177	arg1	transaminase					1187:1198	TNBS-induced alanine transaminase [ALT	1166:1203	TNBS-induced alanine transaminase [ALT	1166:1203	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	4	5	theme	LC27	608:611	arg1	administration					590:603	oral administration	585:603	oral administration of LC27 or LC67 (1 × 109 CFU/mouse)	585:639	First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05] and myeloperoxidase activity [F(5,30) = 56.48, P < 0.05].					
29760423	7	6	theme	P < 0.05	1222:1229	arg1	levels					1292:1297	TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels	1166:1297	TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood	1166:1310	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	8	7	theme	LC27	1397:1400	arg1	mixture					1386:1392	the mixture	1382:1392	the mixture of LC27 and LC67 (0.5 × 109 CFU/mouse, respectively)	1382:1445	Finally, the mixture of LC27 and LC67 (0.5 × 109 CFU/mouse, respectively) synergistically attenuated TNBS- or t-butylhydroperoxide-induced colitis and liver damage.					
29760423	8	8	theme	TNBS-	1474:1478	arg1	colitis					1512:1518	colitis	1512:1518	colitis	1512:1518	Finally, the mixture of LC27 and LC67 (0.5 × 109 CFU/mouse, respectively) synergistically attenuated TNBS- or t-butylhydroperoxide-induced colitis and liver damage.					
29760423	8	9	theme	LC67	1406:1409	arg1	mixture					1386:1392	the mixture	1382:1392	the mixture of LC27 and LC67 (0.5 × 109 CFU/mouse, respectively)	1382:1445	Finally, the mixture of LC27 and LC67 (0.5 × 109 CFU/mouse, respectively) synergistically attenuated TNBS- or t-butylhydroperoxide-induced colitis and liver damage.					
29760423	9	10	theme	LC67	1565:1568	arg1	capability					1542:1551	The capability	1538:1551	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals	1538:1629	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals suggests that these probiotics may synergistically attenuate colitis and liver injury by alleviating gut microbiota imbalance.					
29760423	4	11	theme	TNBS-induced	651:662	arg1	shortening					670:679	TNBS-induced colon shortening [F(5,30) = 100.66	651:697	TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05]	651:708	First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05] and myeloperoxidase activity [F(5,30) = 56.48, P < 0.05].					
29760423	4	12	theme	LC67	616:619	arg1	administration					590:603	oral administration	585:603	oral administration of LC27 or LC67 (1 × 109 CFU/mouse)	585:639	First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05] and myeloperoxidase activity [F(5,30) = 56.48, P < 0.05].					
29760423	0	13	from	Injury	49:54	arg1	Mice					59:62	Mice	59:62	Mice	59:62	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.					
29760423	3	14	from	damage	562:567	arg1	mice					572:575	mice	572:575	mice	572:575	Here, we investigated their effects on 2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis and liver damage in mice.					
29760423	3	14	from	damage	562:567	arg1	colitis					544:550	2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis	496:550	2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis	496:550	Here, we investigated their effects on 2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis and liver damage in mice.					
29760423	9	15	theme	LC27	1556:1559	arg1	capability					1542:1551	The capability	1538:1551	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals	1538:1629	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals suggests that these probiotics may synergistically attenuate colitis and liver injury by alleviating gut microbiota imbalance.					
29760423	4	16	dep	 = 56.48	747:754	arg1	P < 0.05					757:764	P < 0.05	757:764	P < 0.05	757:764	First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05] and myeloperoxidase activity [F(5,30) = 56.48, P < 0.05].					
29760423	7	17	dep	transaminase	1187:1198	arg1	F					1265:1265	F(5,30) = 12.81	1265:1279	F(5,30) = 12.81	1265:1279	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	7	17	dep	transaminase	1187:1198	arg1	P < 0.05					1282:1289	P < 0.05	1282:1289	P < 0.05	1282:1289	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	1	18	from	Disturbances	129:140	arg1	composition					164:174	the gut microbiota composition	145:174	the gut microbiota composition	145:174	Disturbances in the gut microbiota composition are associated with chronic inflammatory diseases of the intestine and the liver.					
29760423	5	19	theme	gut	822:824	arg1	microbiota					826:835	gut microbiota	822:835	gut microbiota	822:835	These probiotics restored TNBS-induced disturbance of gut microbiota, leading to the suppression of Proteobacteria to Bacteroidetes ratio and fecal and blood lipopolysaccharide levels.					
29760423	5	20	theme	Proteobacteria	868:881	arg1	suppression					853:863	the suppression	849:863	the suppression of Proteobacteria	849:881	These probiotics restored TNBS-induced disturbance of gut microbiota, leading to the suppression of Proteobacteria to Bacteroidetes ratio and fecal and blood lipopolysaccharide levels.					
29760423	7	21	theme	aspartate	1236:1244	arg1	transaminase					1246:1257	aspartate transaminase [AST	1236:1262	aspartate transaminase [AST	1236:1262	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	9	22	theme	liver	1704:1708	arg1	injury					1710:1715	liver injury	1704:1715	liver injury	1704:1715	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals suggests that these probiotics may synergistically attenuate colitis and liver injury by alleviating gut microbiota imbalance.					
29760423	6	23	theme	NF-κB	998:1002	arg1	activation					1004:1013	TNBS-induced NF-κB activation	985:1013	TNBS-induced NF-κB activation	985:1013	Second, LC27 and LC67 inhibited TNBS-induced NF-κB activation, reversed TNBS-suppressed tight junction protein expression, and restored Th17/Treg balance.					
29760423	2	24	theme	longum	331:336	arg1	LC67					338:341	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67	282:341	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67	282:341	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	8	25	theme	liver	1524:1528	arg1	damage					1530:1535	liver damage	1524:1535	liver damage	1524:1535	Finally, the mixture of LC27 and LC67 (0.5 × 109 CFU/mouse, respectively) synergistically attenuated TNBS- or t-butylhydroperoxide-induced colitis and liver damage.					
29760423	0	26	theme	Simultaneous	0:11	arg1	Amelioratation					13:26	Simultaneous Amelioratation	0:26	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.	0:127	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.					
29760423	4	27	theme	oral	585:588	arg1	administration					590:603	oral administration	585:603	oral administration of LC27 or LC67 (1 × 109 CFU/mouse)	585:639	First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05] and myeloperoxidase activity [F(5,30) = 56.48, P < 0.05].					
29760423	9	28	theme	reverse	1573:1579	arg1	shift					1606:1610	reverse TNBS-mediated microbiota shift	1573:1610	reverse TNBS-mediated microbiota shift	1573:1610	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals suggests that these probiotics may synergistically attenuate colitis and liver injury by alleviating gut microbiota imbalance.					
29760423	1	29	theme	chronic	196:202	arg1	diseases					217:224	chronic inflammatory diseases	196:224	chronic inflammatory diseases of the intestine and the liver	196:255	Disturbances in the gut microbiota composition are associated with chronic inflammatory diseases of the intestine and the liver.					
29760423	0	30	theme	Colitis	31:37	arg1	Amelioratation					13:26	Simultaneous Amelioratation	0:26	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.	0:127	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.					
29760423	6	31	theme	TNBS-induced	985:996	arg1	activation					1004:1013	TNBS-induced NF-κB activation	985:1013	TNBS-induced NF-κB activation	985:1013	Second, LC27 and LC67 inhibited TNBS-induced NF-κB activation, reversed TNBS-suppressed tight junction protein expression, and restored Th17/Treg balance.					
29760423	7	32	theme	t-butylhydroperoxide-induced	1324:1351	arg1	ALT					1353:1355	t-butylhydroperoxide-induced ALT	1324:1355	t-butylhydroperoxide-induced ALT	1324:1355	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	2	33	dep	Escherichia	357:367	arg1	coli					369:372	coli	369:372	coli	369:372	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	7	34	from	levels	1365:1370	arg1	blood					1306:1310	the blood	1302:1310	the blood	1302:1310	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	1	35	theme	inflammatory	204:215	arg1	diseases					217:224	chronic inflammatory diseases	196:224	chronic inflammatory diseases of the intestine and the liver	196:255	Disturbances in the gut microbiota composition are associated with chronic inflammatory diseases of the intestine and the liver.					
29760423	2	36	theme	LC27	306:309	arg1	LC67					338:341	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67	282:341	Lactobacillus plantarum LC27 and Bifidobacterium longum LC67	282:341	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	3	37	theme	liver	556:560	arg1	damage					562:567	liver damage	556:567	liver damage in mice	556:575	Here, we investigated their effects on 2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis and liver damage in mice.					
29760423	0	38	theme	Liver	43:47	arg1	Injury					49:54	Liver Injury	43:54	Liver Injury	43:54	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.					
29760423	4	39	theme	myeloperoxidase	714:728	arg1	activity					730:737	myeloperoxidase activity	714:737	myeloperoxidase activity	714:737	First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05] and myeloperoxidase activity [F(5,30) = 56.48, P < 0.05].					
29760423	9	40	theme	gut	1732:1734	arg1	imbalance					1747:1755	gut microbiota imbalance	1732:1755	gut microbiota imbalance	1732:1755	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals suggests that these probiotics may synergistically attenuate colitis and liver injury by alleviating gut microbiota imbalance.					
29760423	9	41	theme	microbiota	1595:1604	arg1	shift					1606:1610	reverse TNBS-mediated microbiota shift	1573:1610	reverse TNBS-mediated microbiota shift	1573:1610	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals suggests that these probiotics may synergistically attenuate colitis and liver injury by alleviating gut microbiota imbalance.					
29760423	2	42	theme	NF-κB	412:416	arg1	activation					418:427	lipopolysaccharide-induced NF-κB activation	385:427	lipopolysaccharide-induced NF-κB activation	385:427	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	9	43	theme	microbiota	1736:1745	arg1	imbalance					1747:1755	gut microbiota imbalance	1732:1755	gut microbiota imbalance	1732:1755	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals suggests that these probiotics may synergistically attenuate colitis and liver injury by alleviating gut microbiota imbalance.					
29760423	4	44	dep	shortening	670:679	arg1	 = 56.48					747:754	 = 56.48	747:754	 = 56.48	747:754	First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05] and myeloperoxidase activity [F(5,30) = 56.48, P < 0.05].					
29760423	4	44	dep	shortening	670:679	arg1	P < 0.05					700:707	P < 0.05	700:707	P < 0.05	700:707	First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05] and myeloperoxidase activity [F(5,30) = 56.48, P < 0.05].					
29760423	3	45	from	effects	485:491	arg1	mice					572:575	mice	572:575	mice	572:575	Here, we investigated their effects on 2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis and liver damage in mice.					
29760423	3	45	from	effects	485:491	arg1	colitis					544:550	2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis	496:550	2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis	496:550	Here, we investigated their effects on 2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis and liver damage in mice.					
29760423	2	46	theme	gut	439:441	arg1	inflammation					443:454	gut inflammation	439:454	gut inflammation	439:454	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	5	47	theme	TNBS-induced	794:805	arg1	disturbance					807:817	TNBS-induced disturbance	794:817	TNBS-induced disturbance of gut microbiota	794:835	These probiotics restored TNBS-induced disturbance of gut microbiota, leading to the suppression of Proteobacteria to Bacteroidetes ratio and fecal and blood lipopolysaccharide levels.					
29760423	0	48	from	Mice	59:62	arg1	Amelioratation					13:26	Simultaneous Amelioratation	0:26	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.	0:127	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.					
29760423	7	49	theme	AST	1361:1363	arg1	levels					1365:1370	t-butylhydroperoxide-induced ALT and AST levels	1324:1370	t-butylhydroperoxide-induced ALT and AST levels	1324:1370	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	7	50	from	levels	1292:1297	arg1	blood					1306:1310	the blood	1302:1310	the blood	1302:1310	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	2	51	theme	Escherichia	357:367	arg1	growth					374:379	Escherichia coli growth	357:379	Escherichia coli growth	357:379	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	0	52	theme	Injury	49:54	arg1	Amelioratation					13:26	Simultaneous Amelioratation	0:26	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.	0:127	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.					
29760423	8	53	theme	t-butylhydroperoxide-induced	1483:1510	arg1	colitis					1512:1518	colitis	1512:1518	colitis	1512:1518	Finally, the mixture of LC27 and LC67 (0.5 × 109 CFU/mouse, respectively) synergistically attenuated TNBS- or t-butylhydroperoxide-induced colitis and liver damage.					
29760423	2	54	attach	linked	429:434	arg1	inflammation					443:454	gut inflammation	439:454	gut inflammation	439:454	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	2	54	attach	linked	429:434	arg2	growth					374:379	Escherichia coli growth	357:379	Escherichia coli growth	357:379	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	5	55	theme	microbiota	826:835	arg1	disturbance					807:817	TNBS-induced disturbance	794:817	TNBS-induced disturbance of gut microbiota	794:835	These probiotics restored TNBS-induced disturbance of gut microbiota, leading to the suppression of Proteobacteria to Bacteroidetes ratio and fecal and blood lipopolysaccharide levels.					
29760423	3	56	theme	-induced	535:542	arg1	colitis					544:550	2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis	496:550	2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis	496:550	Here, we investigated their effects on 2,4,6-trinitrobenzesulfonic acid (TNBS)-induced colitis and liver damage in mice.					
29760423	7	57	theme	F	1206:1206	arg1	levels					1292:1297	TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels	1166:1297	TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood	1166:1310	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	7	58	theme	transaminase	1187:1198	arg1	levels					1292:1297	TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels	1166:1297	TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood	1166:1310	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	7	59	theme	ALT	1353:1355	arg1	levels					1365:1370	t-butylhydroperoxide-induced ALT and AST levels	1324:1370	t-butylhydroperoxide-induced ALT and AST levels	1324:1370	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	2	60	theme	preliminary	263:273	arg1	study					275:279	a preliminary study	261:279	a preliminary study	261:279	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	9	61	theme	TNBS-mediated	1581:1593	arg1	shift					1606:1610	reverse TNBS-mediated microbiota shift	1573:1610	reverse TNBS-mediated microbiota shift	1573:1610	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals suggests that these probiotics may synergistically attenuate colitis and liver injury by alleviating gut microbiota imbalance.					
29760423	5	62	theme	Bacteroidetes	886:898	arg1	ratio					900:904	Bacteroidetes ratio and fecal and blood lipopolysaccharide levels	886:950	ratio	900:904	These probiotics restored TNBS-induced disturbance of gut microbiota, leading to the suppression of Proteobacteria to Bacteroidetes ratio and fecal and blood lipopolysaccharide levels.					
29760423	1	63	theme	intestine	233:241	arg1	diseases					217:224	chronic inflammatory diseases	196:224	chronic inflammatory diseases of the intestine and the liver	196:255	Disturbances in the gut microbiota composition are associated with chronic inflammatory diseases of the intestine and the liver.					
29760423	5	64	theme	blood	920:924	arg1	levels					945:950	Bacteroidetes ratio and fecal and blood lipopolysaccharide levels	886:950	levels	945:950	These probiotics restored TNBS-induced disturbance of gut microbiota, leading to the suppression of Proteobacteria to Bacteroidetes ratio and fecal and blood lipopolysaccharide levels.					
29760423	6	65	theme	Th17/Treg	1089:1097	arg1	balance					1099:1105	Th17/Treg balance	1089:1105	Th17/Treg balance	1089:1105	Second, LC27 and LC67 inhibited TNBS-induced NF-κB activation, reversed TNBS-suppressed tight junction protein expression, and restored Th17/Treg balance.					
29760423	8	66	dep	LC27	1397:1400	arg1	CFU/mouse					1422:1430	0.5 × 109 CFU/mouse	1412:1430	0.5 × 109 CFU/mouse	1412:1430	Finally, the mixture of LC27 and LC67 (0.5 × 109 CFU/mouse, respectively) synergistically attenuated TNBS- or t-butylhydroperoxide-induced colitis and liver damage.					
29760423	7	67	with	treatment	1114:1122	arg1	LC27					1129:1132	LC27	1129:1132	LC27	1129:1132	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	7	67	with	treatment	1114:1122	arg1	LC67					1137:1140	LC67	1137:1140	LC67	1137:1140	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	6	68	theme	protein	1056:1062	arg1	expression					1064:1073	TNBS-suppressed tight junction protein expression	1025:1073	TNBS-suppressed tight junction protein expression	1025:1073	Second, LC27 and LC67 inhibited TNBS-induced NF-κB activation, reversed TNBS-suppressed tight junction protein expression, and restored Th17/Treg balance.					
29760423	7	69	theme	transaminase	1246:1257	arg1	levels					1292:1297	TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels	1166:1297	TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood	1166:1310	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	4	70	theme	colon	664:668	arg1	shortening					670:679	TNBS-induced colon shortening [F(5,30) = 100.66	651:697	TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05]	651:708	First, oral administration of LC27 or LC67 (1 × 109 CFU/mouse) inhibited TNBS-induced colon shortening [F(5,30) = 100.66, P < 0.05] and myeloperoxidase activity [F(5,30) = 56.48, P < 0.05].					
29760423	2	71	theme	lipopolysaccharide-induced	385:410	arg1	activation					418:427	lipopolysaccharide-induced NF-κB activation	385:427	lipopolysaccharide-induced NF-κB activation	385:427	In a preliminary study, Lactobacillus plantarum LC27 and Bifidobacterium longum LC67 could inhibit Escherichia coli growth and lipopolysaccharide-induced NF-κB activation linked to gut inflammation.					
29760423	7	72	theme	[AST	1259:1262	arg1	transaminase					1246:1257	aspartate transaminase [AST	1236:1262	aspartate transaminase [AST	1236:1262	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	6	73	theme	junction	1047:1054	arg1	expression					1064:1073	TNBS-suppressed tight junction protein expression	1025:1073	TNBS-suppressed tight junction protein expression	1025:1073	Second, LC27 and LC67 inhibited TNBS-induced NF-κB activation, reversed TNBS-suppressed tight junction protein expression, and restored Th17/Treg balance.					
29760423	7	74	theme	alanine	1179:1185	arg1	transaminase					1187:1198	TNBS-induced alanine transaminase [ALT	1166:1203	TNBS-induced alanine transaminase [ALT	1166:1203	Also, treatment with LC27 or LC67 significantly decreased TNBS-induced alanine transaminase [ALT, F(5,30) = 3.50, P < 0.05] and aspartate transaminase [AST, F(5,30) = 12.81, P < 0.05] levels in the blood, as well as t-butylhydroperoxide-induced ALT and AST levels.					
29760423	0	75	from	Colitis	31:37	arg1	Mice					59:62	Mice	59:62	Mice	59:62	Simultaneous Amelioratation of Colitis and Liver Injury in Mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27.					
29760423	6	76	theme	tight	1041:1045	arg1	expression					1064:1073	TNBS-suppressed tight junction protein expression	1025:1073	TNBS-suppressed tight junction protein expression	1025:1073	Second, LC27 and LC67 inhibited TNBS-induced NF-κB activation, reversed TNBS-suppressed tight junction protein expression, and restored Th17/Treg balance.					
29760423	1	77	theme	gut	149:151	arg1	composition					164:174	the gut microbiota composition	145:174	the gut microbiota composition	145:174	Disturbances in the gut microbiota composition are associated with chronic inflammatory diseases of the intestine and the liver.					
29760423	9	78	theme	damage	1616:1621	arg1	signals					1623:1629	damage signals	1616:1629	damage signals	1616:1629	The capability of LC27 and LC67 to reverse TNBS-mediated microbiota shift and damage signals suggests that these probiotics may synergistically attenuate colitis and liver injury by alleviating gut microbiota imbalance.					
29760423	1	79	theme	liver	251:255	arg1	diseases					217:224	chronic inflammatory diseases	196:224	chronic inflammatory diseases of the intestine and the liver	196:255	Disturbances in the gut microbiota composition are associated with chronic inflammatory diseases of the intestine and the liver.					
29502837	0	0	theme	bean	80:83	arg1	varieties					85:93	bean varieties	80:93	bean varieties	80:93	Seed characteristics and physicochemical properties of powders of 25 edible dry bean varieties.					
29502837	2	1	theme	pasting	484:490	arg1	properties					504:513	pasting and thermal properties	484:513	pasting and thermal properties	484:513	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	1	2	from	varieties	176:184	arg1	variability					131:141	the physicochemical variability	111:141	the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years	111:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	1	2	from	varieties	176:184	arg1	seeds					155:159	dry bean seeds	146:159	dry bean seeds from different varieties grown over distinct crop years	146:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	2	3	theme	bean	355:358	arg1	varieties					360:368	25 edible dry bean varieties	341:368	25 edible dry bean varieties	341:368	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	2	4	theme	dry	351:353	arg1	varieties					360:368	25 edible dry bean varieties	341:368	25 edible dry bean varieties	341:368	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	6	5	used	used	952:955	arg2	information					933:943	The accumulated information	917:943	The accumulated information	917:943	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	3	6	theme	genotype	535:542	arg1	impact					520:525	The impact	516:525	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm)	516:640	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	6	7	theme	breeding	960:967	arg1	programs					969:976	breeding programs	960:976	breeding programs	960:976	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	6	8	theme	crop	1090:1093	arg1	value					1077:1081	the market value	1066:1081	the market value of the crop	1066:1093	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	2	9	theme	proximate	407:415	arg1	composition					417:427	their proximate composition	401:427	their proximate composition	401:427	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	1	10	theme	different	166:174	arg1	varieties					176:184	different varieties	166:184	different varieties grown over distinct crop years	166:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	0	11	theme	powders	55:61	arg1	characteristics					5:19	Seed characteristics	0:19	Seed characteristics	0:19	Seed characteristics and physicochemical properties of powders of 25 edible dry bean varieties.					
29502837	0	11	theme	powders	55:61	arg1	properties					41:50	physicochemical properties	25:50	physicochemical properties	25:50	Seed characteristics and physicochemical properties of powders of 25 edible dry bean varieties.					
29502837	6	12	contain	possessing	999:1008	arg1	lines					993:997	bean lines	988:997	bean lines possessing unique properties	988:1026	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	6	12	contain	possessing	999:1008	arg2	properties					1017:1026	unique properties	1010:1026	unique properties	1010:1026	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	1	13	from	variability	131:141	arg1	seeds					155:159	dry bean seeds	146:159	dry bean seeds from different varieties grown over distinct crop years	146:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	1	13	from	variability	131:141	arg1	varieties					176:184	different varieties	166:184	different varieties grown over distinct crop years	166:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	3	14	theme	bean	530:533	arg1	genotype					535:542	bean genotype	530:542	bean genotype (25 varieties)	530:557	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	3	14	theme	bean	530:533	arg1	varieties					548:556	25 varieties	545:556	25 varieties	545:556	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	6	15	theme	food	1032:1035	arg1	ingredients					1037:1047	food ingredients	1032:1047	food ingredients	1032:1047	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	2	16	theme	thermal	496:502	arg1	properties					504:513	pasting and thermal properties	484:513	pasting and thermal properties	484:513	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	2	17	theme	seed	317:320	arg1	characteristics					322:336	the seed characteristics	313:336	the seed characteristics of 25 edible dry bean varieties	313:368	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	5	18	theme	thermal	817:823	arg1	properties					825:834	Unique pasting and thermal properties	798:834	Unique pasting and thermal properties	798:834	Unique pasting and thermal properties were observed, and genotype and particle size greatly affected these properties.					
29502837	0	19	theme	Seed	0:3	arg1	characteristics					5:19	Seed characteristics	0:19	Seed characteristics	0:19	Seed characteristics and physicochemical properties of powders of 25 edible dry bean varieties.					
29502837	6	20	theme	human	1109:1113	arg1	health					1115:1120	human health	1109:1120	human health	1109:1120	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	0	21	theme	physicochemical	25:39	arg1	properties					41:50	physicochemical properties	25:50	physicochemical properties	25:50	Seed characteristics and physicochemical properties of powders of 25 edible dry bean varieties.					
29502837	6	22	theme	accumulated	921:931	arg1	information					933:943	The accumulated information	917:943	The accumulated information	917:943	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	1	23	theme	distinct	197:204	arg1	years					211:215	distinct crop years	197:215	distinct crop years	197:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	3	24	theme	particle	609:616	arg1	size					618:621	powder particle size	602:621	powder particle size (≤0.5 mm, ≤1.0 mm)	602:640	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	4	25	theme	seed	698:701	arg1	characteristics					703:717	seed characteristics	698:717	seed characteristics	698:717	Statistical differences (P > 0.05) in seed characteristics and in starch, amylose and protein contents were found among the 25 varieties.					
29502837	1	26	theme	crop	206:209	arg1	years					211:215	distinct crop years	197:215	distinct crop years	197:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	4	27	theme	Statistical	660:670	arg1	differences					672:682	Statistical differences	660:682	Statistical differences (P > 0.05) in seed characteristics and in starch, amylose and protein contents	660:761	Statistical differences (P > 0.05) in seed characteristics and in starch, amylose and protein contents were found among the 25 varieties.					
29502837	4	27	theme	Statistical	660:670	arg1	P > 0.05					685:692	P > 0.05	685:692	P > 0.05	685:692	Statistical differences (P > 0.05) in seed characteristics and in starch, amylose and protein contents were found among the 25 varieties.					
29502837	3	28	theme	size	618:621	arg1	impact					520:525	The impact	516:525	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm)	516:640	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	5	29	theme	pasting	805:811	arg1	properties					825:834	Unique pasting and thermal properties	798:834	Unique pasting and thermal properties	798:834	Unique pasting and thermal properties were observed, and genotype and particle size greatly affected these properties.					
29502837	2	30	theme	starch	430:435	arg1	digestibility					437:449	starch digestibility	430:449	starch digestibility	430:449	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	5	31	theme	Unique	798:803	arg1	properties					825:834	Unique pasting and thermal properties	798:834	Unique pasting and thermal properties	798:834	Unique pasting and thermal properties were observed, and genotype and particle size greatly affected these properties.					
29502837	2	32	theme	retention	460:468	arg1	capacity					470:477	solvent retention capacity	452:477	solvent retention capacity	452:477	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	3	33	theme	powder	602:607	arg1	size					618:621	powder particle size	602:621	powder particle size (≤0.5 mm, ≤1.0 mm)	602:640	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	3	34	theme	growing	560:566	arg1	years					590:594	two crop years	581:594	two crop years	581:594	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	3	34	theme	growing	560:566	arg1	environment					568:578	growing environment	560:578	growing environment (two crop years)	560:595	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	6	35	theme	market	1070:1075	arg1	value					1077:1081	the market value	1066:1081	the market value of the crop	1066:1093	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	2	36	theme	edible	344:349	arg1	varieties					360:368	25 edible dry bean varieties	341:368	25 edible dry bean varieties	341:368	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	2	37	theme	solvent	452:458	arg1	capacity					470:477	solvent retention capacity	452:477	solvent retention capacity	452:477	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	3	38	theme	environment	568:578	arg1	impact					520:525	The impact	516:525	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm)	516:640	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	2	39	from	knowledge	388:396	arg1	digestibility					437:449	starch digestibility	430:449	starch digestibility	430:449	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	2	39	from	knowledge	388:396	arg1	capacity					470:477	solvent retention capacity	452:477	solvent retention capacity	452:477	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	2	39	from	knowledge	388:396	arg1	properties					504:513	pasting and thermal properties	484:513	pasting and thermal properties	484:513	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	2	39	from	knowledge	388:396	arg1	composition					417:427	their proximate composition	401:427	their proximate composition	401:427	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	1	40	theme	physicochemical	115:129	arg1	variability					131:141	the physicochemical variability	111:141	the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years	111:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	1	41	from	Information	96:106	arg1	variability					131:141	the physicochemical variability	111:141	the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years	111:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	5	42	theme	particle	868:875	arg1	size					877:880	particle size	868:880	particle size	868:880	Unique pasting and thermal properties were observed, and genotype and particle size greatly affected these properties.					
29502837	1	43	theme	bean	150:153	arg1	seeds					155:159	dry bean seeds	146:159	dry bean seeds from different varieties grown over distinct crop years	146:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	4	44	from	differences	672:682	arg1	amylose					734:740	amylose	734:740	amylose	734:740	Statistical differences (P > 0.05) in seed characteristics and in starch, amylose and protein contents were found among the 25 varieties.					
29502837	4	44	from	differences	672:682	arg1	contents					754:761	protein contents	746:761	protein contents	746:761	Statistical differences (P > 0.05) in seed characteristics and in starch, amylose and protein contents were found among the 25 varieties.					
29502837	4	44	from	differences	672:682	arg1	starch					726:731	starch	726:731	starch	726:731	Statistical differences (P > 0.05) in seed characteristics and in starch, amylose and protein contents were found among the 25 varieties.					
29502837	4	44	from	differences	672:682	arg1	characteristics					703:717	seed characteristics	698:717	seed characteristics	698:717	Statistical differences (P > 0.05) in seed characteristics and in starch, amylose and protein contents were found among the 25 varieties.					
29502837	0	45	theme	edible	69:74	arg1	powders					55:61	powders	55:61	powders of 25 edible	55:74	Seed characteristics and physicochemical properties of powders of 25 edible dry bean varieties.					
29502837	6	46	theme	bean	988:991	arg1	lines					993:997	bean lines	988:997	bean lines possessing unique properties	988:1026	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	3	47	theme	crop	585:588	arg1	years					590:594	two crop years	581:594	two crop years	581:594	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	3	47	theme	crop	585:588	arg1	environment					568:578	growing environment	560:578	growing environment (two crop years)	560:595	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	6	48	theme	unique	1010:1015	arg1	properties					1017:1026	unique properties	1010:1026	unique properties	1010:1026	The accumulated information can be used in breeding programs to select bean lines possessing unique properties for food ingredients while increasing the market value of the crop and enhancing human health.					
29502837	1	49	theme	dry	146:148	arg1	seeds					155:159	dry bean seeds	146:159	dry bean seeds from different varieties grown over distinct crop years	146:215	Information on the physicochemical variability in dry bean seeds from different varieties grown over distinct crop years is lacking.					
29502837	4	50	theme	protein	746:752	arg1	contents					754:761	protein contents	746:761	protein contents	746:761	Statistical differences (P > 0.05) in seed characteristics and in starch, amylose and protein contents were found among the 25 varieties.					
29502837	2	51	theme	varieties	360:368	arg1	characteristics					322:336	the seed characteristics	313:336	the seed characteristics of 25 edible dry bean varieties	313:368	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	2	51	theme	varieties	360:368	arg1	environment					297:307	the environment	293:307	the environment	293:307	This study was designed to investigate the relationship between the environment and the seed characteristics of 25 edible dry bean varieties and to expand the knowledge on their proximate composition, starch digestibility, solvent retention capacity, and pasting and thermal properties.					
29502837	3	52	dep	size	618:621	arg1	≤0.5 mm					624:630	≤0.5 mm	624:630	≤0.5 mm	624:630	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
29502837	3	52	dep	size	618:621	arg1	≤1.0 mm					633:639	≤1.0 mm	633:639	≤1.0 mm	633:639	The impact of bean genotype (25 varieties), growing environment (two crop years), and powder particle size (≤0.5 mm, ≤1.0 mm) was investigated.					
31563306	10	0	theme	factors	1419:1425	arg1	effects					1387:1393	fixed effects	1381:1393	fixed effects of previously mentioned factors	1381:1425	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	10	0	theme	factors	1419:1425	arg1	effect					1466:1471	the random animal additive genetic effect	1431:1471	the random animal additive genetic effect	1431:1471	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	20	1	theme	genetic	2598:2604	arg1	selection					2606:2614	genetic selection	2598:2614	genetic selection	2598:2614	In conclusion, colostrum quality traits are heritable and can be amended with genetic selection.					
31563306	10	2	theme	genetic	1458:1464	arg1	effect					1466:1471	the random animal additive genetic effect	1431:1471	the random animal additive genetic effect	1431:1471	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	20	3	from	heritable	2564:2572	arg1	conclusion					2523:2532	conclusion	2523:2532	conclusion	2523:2532	In conclusion, colostrum quality traits are heritable and can be amended with genetic selection.					
31563306	10	4	theme	animal	1442:1447	arg1	effect					1466:1471	the random animal additive genetic effect	1431:1471	the random animal additive genetic effect	1431:1471	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	11	5	theme	total	1542:1546	arg1	number					1555:1560	the total animal number	1538:1560	the total animal number	1538:1560	All available pedigrees were included in the analysis, bringing the total animal number to 5,662.					
31563306	10	6	theme	quality	1311:1317	arg1	traits					1319:1324	quality traits	1311:1324	quality traits	1311:1324	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	7	7	theme	digital	827:833	arg1	refractometer					840:852	a digital Brix refractometer	825:852	a digital Brix refractometer	825:852	Colostrum total solids (TS) content was determined on-farm using a digital Brix refractometer.					
31563306	14	8	theme	TS	1790:1791	arg1	contents					1820:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents	1758:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents	1758:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	12	9	theme	components	1598:1607	arg1	Estimates					1572:1580	Estimates	1572:1580	Estimates of (co)variance components	1572:1607	Estimates of (co)variance components were used to calculate heritability for each trait.					
31563306	17	10	theme	content	2200:2206	arg1	correlation					2182:2192	The genetic correlation	2170:2192	The genetic correlation of TS content measured on-farm with colostrum protein	2170:2246	The genetic correlation of TS content measured on-farm with colostrum protein was practically unity, whereas the correlation with energy content was moderate (0.61).					
31563306	17	10	theme	content	2200:2206	arg1	unity					2264:2268	unity	2264:2268	unity	2264:2268	The genetic correlation of TS content measured on-farm with colostrum protein was practically unity, whereas the correlation with energy content was moderate (0.61).					
31563306	14	11	theme	colostrum	1951:1959	arg1	yield					1961:1965	mean colostrum yield	1946:1965	mean colostrum yield	1946:1965	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	19	12	theme	other	2506:2510	arg1	traits					2512:2517	the other traits	2502:2517	the other traits	2502:2517	Colostrum yield was not correlated genetically with any of the other traits.					
31563306	5	13	theme	body	628:631	arg1	condition					633:641	body condition	628:641	body condition	628:641	All cows were clinically examined on the day of calving and scored for body condition.					
31563306	16	14	theme	genetic	2120:2126	arg1	correlations					2143:2154	Several significant genetic and phenotypic correlations	2100:2154	Several significant genetic and phenotypic correlations	2100:2154	Several significant genetic and phenotypic correlations were derived.					
31563306	2	15	theme	laboratory-based	294:309	arg1	traits					342:347	laboratory-based and on-farm-measured colostrum traits	294:347	laboratory-based and on-farm-measured colostrum traits	294:347	The secondary objective was to investigate genetic and phenotypic correlations among laboratory-based and on-farm-measured colostrum traits.					
31563306	12	16	used	used	1614:1617	arg2	Estimates					1572:1580	Estimates	1572:1580	Estimates of (co)variance components	1572:1607	Estimates of (co)variance components were used to calculate heritability for each trait.					
31563306	1	17	theme	traits	164:169	arg1	background					122:131	the genetic background	110:131	the genetic background of colostrum yield and quality traits	110:169	The main objective of this study was to assess the genetic background of colostrum yield and quality traits after calving in Holstein dairy cows.					
31563306	8	18	theme	National	989:996	arg1	2001					1016:1019	2001	1016:1019	2001	1016:1019	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	8	18	theme	National	989:996	arg1	Council					1007:1013	National Research Council	989:1013	National Research Council (2001) equations	989:1030	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	20	19	theme	quality	2545:2551	arg1	traits					2553:2558	colostrum quality traits	2535:2558	colostrum quality traits	2535:2558	In conclusion, colostrum quality traits are heritable and can be amended with genetic selection.					
31563306	17	20	theme	colostrum	2230:2238	arg1	protein					2240:2246	colostrum protein	2230:2246	colostrum protein	2230:2246	The genetic correlation of TS content measured on-farm with colostrum protein was practically unity, whereas the correlation with energy content was moderate (0.61).					
31563306	20	21	from	conclusion	2523:2532	arg1	heritable					2564:2572	heritable	2564:2572	heritable	2564:2572	In conclusion, colostrum quality traits are heritable and can be amended with genetic selection.					
31563306	14	22	theme	0.3	1930:1932	arg1	±					1928:1928	±	1928:1928	±	1928:1928	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	1	23	theme	Holstein	188:195	arg1	cows					203:206	Holstein dairy cows	188:206	Holstein dairy cows	188:206	The main objective of this study was to assess the genetic background of colostrum yield and quality traits after calving in Holstein dairy cows.					
31563306	4	24	theme	healthy	448:454	arg1	cows					465:468	1,074 healthy Holstein cows	442:468	1,074 healthy Holstein cows	442:468	A total of 1,074 healthy Holstein cows with detailed pedigree information were examined from February 2015 to September 2016.					
31563306	14	25	theme	1.35	1923:1926	arg1	±					1928:1928	±	1928:1928	±	1928:1928	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	14	26	dep	±	1839:1839	arg1	4.7					1841:1843	4.7	1841:1843	4.7	1841:1843	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	14	26	dep	±	1839:1839	arg1	±					1850:1850	6.4 ± 3.3	1846:1854	6.4 ± 3.3	1846:1854	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	14	26	dep	±	1839:1839	arg1	%					1882:1882	2.2 ± 0.7%	1873:1882	2.2 ± 0.7%	1873:1882	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	14	26	dep	±	1839:1839	arg1	±					1862:1862	17.8 ± 4.0	1857:1866	17.8 ± 4.0	1857:1866	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	13	27	theme	bivariate	1717:1725	arg1	analysis					1727:1734	bivariate analysis	1717:1734	bivariate analysis using the same model	1717:1755	Correlations among colostrum traits were estimated with bivariate analysis using the same model.					
31563306	12	28	theme	variance	1589:1596	arg1	components					1598:1607	(co)variance components	1585:1607	(co)variance components	1585:1607	Estimates of (co)variance components were used to calculate heritability for each trait.					
31563306	9	29	theme	calving	1181:1187	arg1	yield					1258:1262	milk yield	1253:1262	milk yield	1253:1262	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	29	theme	calving	1181:1187	arg1	length					1044:1049	Dry period length	1033:1049	Dry period length (for cows of parity ≥2)	1033:1073	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	29	theme	calving	1181:1187	arg1	interval					1195:1202	time interval	1190:1202	time interval between calving and first colostrum milking	1190:1246	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	29	theme	calving	1181:1187	arg1	number					1163:1168	parity number	1156:1168	parity number	1156:1168	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	29	theme	calving	1181:1187	arg1	age					1140:1142	age	1140:1142	age at calving	1140:1153	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	29	theme	calving	1181:1187	arg1	yield					1081:1085	milk yield	1076:1085	milk yield of previous 305-d lactation (for cows of parity ≥2)	1076:1137	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	29	theme	calving	1181:1187	arg1	season					1171:1176	season	1171:1176	season of calving	1171:1187	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	2	30	theme	secondary	213:221	arg1	objective					223:231	The secondary objective	209:231	The secondary objective	209:231	The secondary objective was to investigate genetic and phenotypic correlations among laboratory-based and on-farm-measured colostrum traits.					
31563306	14	31	theme	±	1976:1976	arg1	kg					1983:1984	6.18 ± 3.77 kg	1971:1984	6.18 ± 3.77 kg	1971:1984	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	9	32	theme	time	1190:1193	arg1	interval					1195:1202	time interval	1190:1202	time interval between calving and first colostrum milking	1190:1246	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	8	33	theme	Council	1007:1013	arg1	equations					1022:1030	National Research Council (2001) equations	989:1030	National Research Council (2001) equations	989:1030	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	1	34	theme	colostrum	136:144	arg1	yield					146:150	colostrum yield	136:150	colostrum yield	136:150	The main objective of this study was to assess the genetic background of colostrum yield and quality traits after calving in Holstein dairy cows.					
31563306	14	35	theme	energy	1904:1909	arg1	content					1911:1917	mean energy content	1899:1917	mean energy content	1899:1917	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	17	36	theme	energy	2300:2305	arg1	content					2307:2313	energy content	2300:2313	energy content	2300:2313	The genetic correlation of TS content measured on-farm with colostrum protein was practically unity, whereas the correlation with energy content was moderate (0.61).					
31563306	9	37	theme	period	1037:1042	arg1	length					1044:1049	Dry period length	1033:1049	Dry period length (for cows of parity ≥2)	1033:1073	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	8	38	theme	fat	865:867	arg1	contents					891:898	Colostrum fat, protein, and lactose contents	855:898	Colostrum fat, protein, and lactose contents	855:898	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	4	39	theme	cows	465:468	arg1	total					433:437	A total	431:437	A total of 1,074 healthy Holstein cows with detailed pedigree information	431:503	A total of 1,074 healthy Holstein cows with detailed pedigree information were examined from February 2015 to September 2016.					
31563306	10	40	theme	mixed	1358:1362	arg1	model					1364:1368	a univariate mixed model	1345:1368	a univariate mixed model	1345:1368	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	6	41	theme	representative	686:699	arg1	sample					725:730	a representative and composite colostrum sample	684:730	a representative and composite colostrum sample	684:730	All 4 quarters were machine-milked, and a representative and composite colostrum sample was collected and examined.					
31563306	1	42	theme	main	67:70	arg1	objective					72:80	The main objective	63:80	The main objective of this study	63:94	The main objective of this study was to assess the genetic background of colostrum yield and quality traits after calving in Holstein dairy cows.					
31563306	8	43	theme	protein	870:876	arg1	contents					891:898	Colostrum fat, protein, and lactose contents	855:898	Colostrum fat, protein, and lactose contents	855:898	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	14	44	theme	6.18	1971:1974	arg1	kg					1983:1984	6.18 ± 3.77 kg	1971:1984	6.18 ± 3.77 kg	1971:1984	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	3	45	theme	commercial	380:389	arg1	herds					397:401	10 commercial dairy herds	377:401	10 commercial dairy herds located in northern Greece	377:428	The study was conducted in 10 commercial dairy herds located in northern Greece.					
31563306	4	46	theme	detailed	475:482	arg1	information					493:503	detailed pedigree information	475:503	detailed pedigree information	475:503	A total of 1,074 healthy Holstein cows with detailed pedigree information were examined from February 2015 to September 2016.					
31563306	18	47	theme	TS	2380:2381	arg1	content					2383:2389	TS content	2380:2389	TS content	2380:2389	Fat content had no genetic correlation with TS content; their phenotypic correlation was positive and low.					
31563306	0	48	theme	Genetic	0:6	arg1	parameters					8:17	Genetic parameters	0:17	Genetic parameters of colostrum traits in Holstein dairy cows	0:60	Genetic parameters of colostrum traits in Holstein dairy cows.					
31563306	9	49	theme	colostrum	1230:1238	arg1	milking					1240:1246	first colostrum milking	1224:1246	first colostrum milking	1224:1246	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	10	50	theme	fixed	1381:1385	arg1	effects					1387:1393	fixed effects	1381:1393	fixed effects of previously mentioned factors	1381:1425	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	18	51	with	correlation	2363:2373	arg1	content					2383:2389	TS content	2380:2389	TS content	2380:2389	Fat content had no genetic correlation with TS content; their phenotypic correlation was positive and low.					
31563306	9	52	theme	parity	1064:1069	arg1	cows					1056:1059	cows	1056:1059	cows of parity ≥2	1056:1072	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	4	53	with	total	433:437	arg1	information					493:503	detailed pedigree information	475:503	detailed pedigree information	475:503	A total of 1,074 healthy Holstein cows with detailed pedigree information were examined from February 2015 to September 2016.					
31563306	1	54	theme	study	90:94	arg1	objective					72:80	The main objective	63:80	The main objective of this study	63:94	The main objective of this study was to assess the genetic background of colostrum yield and quality traits after calving in Holstein dairy cows.					
31563306	18	55	theme	phenotypic	2398:2407	arg1	correlation					2409:2419	their phenotypic correlation	2392:2419	their phenotypic correlation	2392:2419	Fat content had no genetic correlation with TS content; their phenotypic correlation was positive and low.					
31563306	0	56	theme	traits	32:37	arg1	parameters					8:17	Genetic parameters	0:17	Genetic parameters of colostrum traits in Holstein dairy cows	0:60	Genetic parameters of colostrum traits in Holstein dairy cows.					
31563306	7	57	theme	solids	776:781	arg1	content					788:794	Colostrum total solids (TS) content	760:794	Colostrum total solids (TS) content	760:794	Colostrum total solids (TS) content was determined on-farm using a digital Brix refractometer.					
31563306	19	58	theme	Colostrum	2443:2451	arg1	yield					2453:2457	Colostrum yield	2443:2457	Colostrum yield	2443:2457	Colostrum yield was not correlated genetically with any of the other traits.					
31563306	9	59	theme	milk	1253:1256	arg1	yield					1258:1262	milk yield	1253:1262	milk yield	1253:1262	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	10	60	theme	mentioned	1409:1417	arg1	factors					1419:1425	previously mentioned factors	1398:1425	previously mentioned factors	1398:1425	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	17	61	with	on-farm	2217:2223	arg1	protein					2240:2246	colostrum protein	2230:2246	colostrum protein	2230:2246	The genetic correlation of TS content measured on-farm with colostrum protein was practically unity, whereas the correlation with energy content was moderate (0.61).					
31563306	9	62	theme	milk	1076:1079	arg1	yield					1081:1085	milk yield	1076:1085	milk yield of previous 305-d lactation (for cows of parity ≥2)	1076:1137	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	0	63	theme	Holstein	42:49	arg1	cows					57:60	Holstein dairy cows	42:60	Holstein dairy cows	42:60	Genetic parameters of colostrum traits in Holstein dairy cows.					
31563306	7	64	theme	Colostrum	760:768	arg1	solids					776:781	Colostrum total solids	760:781	Colostrum total solids (TS) content	760:794	Colostrum total solids (TS) content was determined on-farm using a digital Brix refractometer.					
31563306	7	64	theme	Colostrum	760:768	arg1	TS					784:785	TS	784:785	TS	784:785	Colostrum total solids (TS) content was determined on-farm using a digital Brix refractometer.					
31563306	17	65	theme	genetic	2174:2180	arg1	correlation					2182:2192	The genetic correlation	2170:2192	The genetic correlation of TS content measured on-farm with colostrum protein	2170:2246	The genetic correlation of TS content measured on-farm with colostrum protein was practically unity, whereas the correlation with energy content was moderate (0.61).					
31563306	17	65	theme	genetic	2174:2180	arg1	unity					2264:2268	unity	2264:2268	unity	2264:2268	The genetic correlation of TS content measured on-farm with colostrum protein was practically unity, whereas the correlation with energy content was moderate (0.61).					
31563306	16	66	theme	Several	2100:2106	arg1	correlations					2143:2154	Several significant genetic and phenotypic correlations	2100:2154	Several significant genetic and phenotypic correlations	2100:2154	Several significant genetic and phenotypic correlations were derived.					
31563306	18	67	contain	had	2348:2350	arg1	content					2340:2346	Fat content	2336:2346	Fat content	2336:2346	Fat content had no genetic correlation with TS content; their phenotypic correlation was positive and low.					
31563306	18	67	contain	had	2348:2350	arg2	correlation					2363:2373	no genetic correlation	2352:2373	no genetic correlation with TS content	2352:2389	Fat content had no genetic correlation with TS content; their phenotypic correlation was positive and low.					
31563306	2	68	theme	colostrum	332:340	arg1	traits					342:347	laboratory-based and on-farm-measured colostrum traits	294:347	laboratory-based and on-farm-measured colostrum traits	294:347	The secondary objective was to investigate genetic and phenotypic correlations among laboratory-based and on-farm-measured colostrum traits.					
31563306	1	69	theme	dairy	197:201	arg1	cows					203:206	Holstein dairy cows	188:206	Holstein dairy cows	188:206	The main objective of this study was to assess the genetic background of colostrum yield and quality traits after calving in Holstein dairy cows.					
31563306	14	70	theme	protein	1799:1805	arg1	contents					1820:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents	1758:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents	1758:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	8	71	theme	milk	934:937	arg1	analyzer					939:946	an infrared milk analyzer	922:946	an infrared milk analyzer	922:946	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	10	72	theme	random	1435:1440	arg1	effect					1466:1471	the random animal additive genetic effect	1431:1471	the random animal additive genetic effect	1431:1471	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	9	73	theme	305-d	1099:1103	arg1	lactation					1105:1113	previous 305-d lactation	1090:1113	previous 305-d lactation (for cows of parity ≥2)	1090:1137	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	14	74	theme	fat	1794:1796	arg1	contents					1820:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents	1758:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents	1758:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	18	75	theme	Fat	2336:2338	arg1	content					2340:2346	Fat content	2336:2346	Fat content	2336:2346	Fat content had no genetic correlation with TS content; their phenotypic correlation was positive and low.					
31563306	17	76	with	correlation	2283:2293	arg1	content					2307:2313	energy content	2300:2313	energy content	2300:2313	The genetic correlation of TS content measured on-farm with colostrum protein was practically unity, whereas the correlation with energy content was moderate (0.61).					
31563306	6	77	theme	composite	705:713	arg1	sample					725:730	a representative and composite colostrum sample	684:730	a representative and composite colostrum sample	684:730	All 4 quarters were machine-milked, and a representative and composite colostrum sample was collected and examined.					
31563306	8	78	theme	energy	953:958	arg1	content					960:966	energy content	953:966	energy content	953:966	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	2	79	theme	phenotypic	264:273	arg1	correlations					275:286	genetic and phenotypic correlations	252:286	genetic and phenotypic correlations among laboratory-based and on-farm-measured colostrum traits	252:347	The secondary objective was to investigate genetic and phenotypic correlations among laboratory-based and on-farm-measured colostrum traits.					
31563306	10	80	dep	trait	1284:1288	arg1	yield					1301:1305	colostrum yield	1291:1305	colostrum yield	1291:1305	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	10	80	dep	trait	1284:1288	arg1	traits					1319:1324	quality traits	1311:1324	quality traits	1311:1324	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	10	81	theme	additive	1449:1456	arg1	effect					1466:1471	the random animal additive genetic effect	1431:1471	the random animal additive genetic effect	1431:1471	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	9	82	theme	parity	1128:1133	arg1	cows					1120:1123	cows	1120:1123	cows of parity ≥2	1120:1136	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	2	83	theme	genetic	252:258	arg1	correlations					275:286	genetic and phenotypic correlations	252:286	genetic and phenotypic correlations among laboratory-based and on-farm-measured colostrum traits	252:347	The secondary objective was to investigate genetic and phenotypic correlations among laboratory-based and on-farm-measured colostrum traits.					
31563306	14	84	theme	colostrum	1780:1788	arg1	TS					1790:1791	Mean percentage (±SD) colostrum TS	1758:1791	Mean percentage (±SD) colostrum TS	1758:1791	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	1	85	theme	yield	146:150	arg1	traits					164:169	colostrum yield and quality traits	136:169	colostrum yield and quality traits	136:169	The main objective of this study was to assess the genetic background of colostrum yield and quality traits after calving in Holstein dairy cows.					
31563306	9	86	from	calving	1147:1153	arg1	yield					1258:1262	milk yield	1253:1262	milk yield	1253:1262	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	86	from	calving	1147:1153	arg1	length					1044:1049	Dry period length	1033:1049	Dry period length (for cows of parity ≥2)	1033:1073	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	86	from	calving	1147:1153	arg1	interval					1195:1202	time interval	1190:1202	time interval between calving and first colostrum milking	1190:1246	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	86	from	calving	1147:1153	arg1	number					1163:1168	parity number	1156:1168	parity number	1156:1168	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	86	from	calving	1147:1153	arg1	age					1140:1142	age	1140:1142	age at calving	1140:1153	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	86	from	calving	1147:1153	arg1	yield					1081:1085	milk yield	1076:1085	milk yield of previous 305-d lactation (for cows of parity ≥2)	1076:1137	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	86	from	calving	1147:1153	arg1	season					1171:1176	season	1171:1176	season of calving	1171:1187	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	17	87	theme	TS	2197:2198	arg1	content					2200:2206	TS content	2197:2206	TS content measured on-farm with colostrum protein	2197:2246	The genetic correlation of TS content measured on-farm with colostrum protein was practically unity, whereas the correlation with energy content was moderate (0.61).					
31563306	3	88	from	Greece	423:428	arg1	located					403:409	located	403:409	located	403:409	The study was conducted in 10 commercial dairy herds located in northern Greece.					
31563306	11	89	theme	animal	1548:1553	arg1	number					1555:1560	the total animal number	1538:1560	the total animal number	1538:1560	All available pedigrees were included in the analysis, bringing the total animal number to 5,662.					
31563306	7	90	theme	Brix	835:838	arg1	refractometer					840:852	a digital Brix refractometer	825:852	a digital Brix refractometer	825:852	Colostrum total solids (TS) content was determined on-farm using a digital Brix refractometer.					
31563306	1	91	theme	quality	156:162	arg1	traits					164:169	colostrum yield and quality traits	136:169	colostrum yield and quality traits	136:169	The main objective of this study was to assess the genetic background of colostrum yield and quality traits after calving in Holstein dairy cows.					
31563306	8	92	theme	Research	998:1005	arg1	2001					1016:1019	2001	1016:1019	2001	1016:1019	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	8	92	theme	Research	998:1005	arg1	Council					1007:1013	National Research Council	989:1013	National Research Council (2001) equations	989:1030	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	14	93	theme	mean	1946:1949	arg1	yield					1961:1965	mean colostrum yield	1946:1965	mean colostrum yield	1946:1965	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	10	94	theme	colostrum	1291:1299	arg1	yield					1301:1305	colostrum yield	1291:1305	colostrum yield	1291:1305	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	14	95	theme	±SD	1775:1777	arg1	TS					1790:1791	Mean percentage (±SD) colostrum TS	1758:1791	Mean percentage (±SD) colostrum TS	1758:1791	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	16	96	theme	phenotypic	2132:2141	arg1	correlations					2143:2154	Several significant genetic and phenotypic correlations	2100:2154	Several significant genetic and phenotypic correlations	2100:2154	Several significant genetic and phenotypic correlations were derived.					
31563306	14	97	theme	percentage	1763:1772	arg1	TS					1790:1791	Mean percentage (±SD) colostrum TS	1758:1791	Mean percentage (±SD) colostrum TS	1758:1791	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	4	98	theme	Holstein	456:463	arg1	cows					465:468	1,074 healthy Holstein cows	442:468	1,074 healthy Holstein cows	442:468	A total of 1,074 healthy Holstein cows with detailed pedigree information were examined from February 2015 to September 2016.					
31563306	9	99	theme	parity	1156:1161	arg1	number					1163:1168	parity number	1156:1168	parity number	1156:1168	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	15	100	theme	Heritability	1987:1998	arg1	estimates					2000:2008	Heritability estimates	1987:2008	Heritability estimates for the above colostrum traits	1987:2039	Heritability estimates for the above colostrum traits were 0.27, 0.21, 0.19, 0.15, 0.22, and 0.04, respectively.					
31563306	16	101	theme	significant	2108:2118	arg1	correlations					2143:2154	Several significant genetic and phenotypic correlations	2100:2154	Several significant genetic and phenotypic correlations	2100:2154	Several significant genetic and phenotypic correlations were derived.					
31563306	20	102	theme	colostrum	2535:2543	arg1	traits					2553:2558	colostrum quality traits	2535:2558	colostrum quality traits	2535:2558	In conclusion, colostrum quality traits are heritable and can be amended with genetic selection.					
31563306	1	103	theme	genetic	114:120	arg1	background					122:131	the genetic background	110:131	the genetic background of colostrum yield and quality traits	110:169	The main objective of this study was to assess the genetic background of colostrum yield and quality traits after calving in Holstein dairy cows.					
31563306	3	104	from	located	403:409	arg1	Greece					423:428	northern Greece	414:428	northern Greece	414:428	The study was conducted in 10 commercial dairy herds located in northern Greece.					
31563306	0	105	from	parameters	8:17	arg1	cows					57:60	Holstein dairy cows	42:60	Holstein dairy cows	42:60	Genetic parameters of colostrum traits in Holstein dairy cows.					
31563306	9	106	theme	Dry	1033:1035	arg1	length					1044:1049	Dry period length	1033:1049	Dry period length (for cows of parity ≥2)	1033:1073	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	4	107	dep	February	524:531	arg1	to					538:539	to	538:539	to	538:539	A total of 1,074 healthy Holstein cows with detailed pedigree information were examined from February 2015 to September 2016.					
31563306	4	107	dep	February	524:531	arg1	September					541:549	September	541:549	September	541:549	A total of 1,074 healthy Holstein cows with detailed pedigree information were examined from February 2015 to September 2016.					
31563306	9	108	theme	previous	1090:1097	arg1	lactation					1105:1113	previous 305-d lactation	1090:1113	previous 305-d lactation (for cows of parity ≥2)	1090:1137	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	18	109	theme	genetic	2355:2361	arg1	correlation					2363:2373	no genetic correlation	2352:2373	no genetic correlation with TS content	2352:2389	Fat content had no genetic correlation with TS content; their phenotypic correlation was positive and low.					
31563306	14	110	theme	mean	1899:1902	arg1	content					1911:1917	mean energy content	1899:1917	mean energy content	1899:1917	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	5	111	theme	calving	605:611	arg1	day					598:600	the day	594:600	the day of calving	594:611	All cows were clinically examined on the day of calving and scored for body condition.					
31563306	8	112	theme	Colostrum	855:863	arg1	fat					865:867	Colostrum fat	855:867	Colostrum fat	855:867	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	4	113	theme	pedigree	484:491	arg1	information					493:503	detailed pedigree information	475:503	detailed pedigree information	475:503	A total of 1,074 healthy Holstein cows with detailed pedigree information were examined from February 2015 to September 2016.					
31563306	10	114	theme	univariate	1347:1356	arg1	model					1364:1368	a univariate mixed model	1345:1368	a univariate mixed model	1345:1368	Each trait (colostrum yield and quality traits) was analyzed with a univariate mixed model, including fixed effects of previously mentioned factors and the random animal additive genetic effect.					
31563306	9	115	theme	first	1224:1228	arg1	milking					1240:1246	first colostrum milking	1224:1246	first colostrum milking	1224:1246	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	13	116	theme	colostrum	1680:1688	arg1	traits					1690:1695	colostrum traits	1680:1695	colostrum traits	1680:1695	Correlations among colostrum traits were estimated with bivariate analysis using the same model.					
31563306	3	117	theme	dairy	391:395	arg1	herds					397:401	10 commercial dairy herds	377:401	10 commercial dairy herds located in northern Greece	377:428	The study was conducted in 10 commercial dairy herds located in northern Greece.					
31563306	0	118	theme	colostrum	22:30	arg1	traits					32:37	colostrum traits	22:37	colostrum traits	22:37	Genetic parameters of colostrum traits in Holstein dairy cows.					
31563306	14	119	theme	±	1877:1877	arg1	%					1882:1882	2.2 ± 0.7%	1873:1882	2.2 ± 0.7%	1873:1882	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	13	120	theme	same	1746:1749	arg1	model					1751:1755	the same model	1742:1755	the same model	1742:1755	Correlations among colostrum traits were estimated with bivariate analysis using the same model.					
31563306	8	121	theme	lactose	883:889	arg1	contents					891:898	Colostrum fat, protein, and lactose contents	855:898	Colostrum fat, protein, and lactose contents	855:898	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	3	122	theme	northern	414:421	arg1	Greece					423:428	northern Greece	414:428	northern Greece	414:428	The study was conducted in 10 commercial dairy herds located in northern Greece.					
31563306	11	123	theme	available	1478:1486	arg1	pedigrees					1488:1496	All available pedigrees	1474:1496	All available pedigrees	1474:1496	All available pedigrees were included in the analysis, bringing the total animal number to 5,662.					
31563306	0	124	theme	dairy	51:55	arg1	cows					57:60	Holstein dairy cows	42:60	Holstein dairy cows	42:60	Genetic parameters of colostrum traits in Holstein dairy cows.					
31563306	15	125	theme	colostrum	2024:2032	arg1	traits					2034:2039	the above colostrum traits	2014:2039	the above colostrum traits	2014:2039	Heritability estimates for the above colostrum traits were 0.27, 0.21, 0.19, 0.15, 0.22, and 0.04, respectively.					
31563306	14	126	theme	lactose	1812:1818	arg1	contents					1820:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents	1758:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents	1758:1827	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	3	127	theme	located	403:409	arg1	herds					397:401	10 commercial dairy herds	377:401	10 commercial dairy herds located in northern Greece	377:428	The study was conducted in 10 commercial dairy herds located in northern Greece.					
31563306	15	128	theme	above	2018:2022	arg1	traits					2034:2039	the above colostrum traits	2014:2039	the above colostrum traits	2014:2039	Heritability estimates for the above colostrum traits were 0.27, 0.21, 0.19, 0.15, 0.22, and 0.04, respectively.					
31563306	7	129	theme	total	770:774	arg1	solids					776:781	Colostrum total solids	760:781	Colostrum total solids (TS) content	760:794	Colostrum total solids (TS) content was determined on-farm using a digital Brix refractometer.					
31563306	7	129	theme	total	770:774	arg1	TS					784:785	TS	784:785	TS	784:785	Colostrum total solids (TS) content was determined on-farm using a digital Brix refractometer.					
31563306	6	130	theme	colostrum	715:723	arg1	sample					725:730	a representative and composite colostrum sample	684:730	a representative and composite colostrum sample	684:730	All 4 quarters were machine-milked, and a representative and composite colostrum sample was collected and examined.					
31563306	7	131	dep	determined	800:809	arg1	using					819:823	using	819:823	using a digital Brix refractometer	819:852	Colostrum total solids (TS) content was determined on-farm using a digital Brix refractometer.					
31563306	2	132	theme	on-farm-measured	315:330	arg1	traits					342:347	laboratory-based and on-farm-measured colostrum traits	294:347	laboratory-based and on-farm-measured colostrum traits	294:347	The secondary objective was to investigate genetic and phenotypic correlations among laboratory-based and on-farm-measured colostrum traits.					
31563306	14	133	theme	Mean	1758:1761	arg1	TS					1790:1791	Mean percentage (±SD) colostrum TS	1758:1791	Mean percentage (±SD) colostrum TS	1758:1791	Mean percentage (±SD) colostrum TS, fat, protein, and lactose contents were 25.8 ± 4.7, 6.4 ± 3.3, 17.8 ± 4.0, and 2.2 ± 0.7%, respectively; mean energy content was 1.35 ± 0.3 Mcal/kg and mean colostrum yield was 6.18 ± 3.77 kg.					
31563306	8	134	theme	infrared	925:932	arg1	analyzer					939:946	an infrared milk analyzer	922:946	an infrared milk analyzer	922:946	Colostrum fat, protein, and lactose contents were determined using an infrared milk analyzer, and energy content was calculated using National Research Council (2001) equations.					
31563306	9	135	theme	lactation	1105:1113	arg1	yield					1258:1262	milk yield	1253:1262	milk yield	1253:1262	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	135	theme	lactation	1105:1113	arg1	length					1044:1049	Dry period length	1033:1049	Dry period length (for cows of parity ≥2)	1033:1073	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	135	theme	lactation	1105:1113	arg1	interval					1195:1202	time interval	1190:1202	time interval between calving and first colostrum milking	1190:1246	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	135	theme	lactation	1105:1113	arg1	number					1163:1168	parity number	1156:1168	parity number	1156:1168	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	135	theme	lactation	1105:1113	arg1	age					1140:1142	age	1140:1142	age at calving	1140:1153	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	135	theme	lactation	1105:1113	arg1	yield					1081:1085	milk yield	1076:1085	milk yield of previous 305-d lactation (for cows of parity ≥2)	1076:1137	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31563306	9	135	theme	lactation	1105:1113	arg1	season					1171:1176	season	1171:1176	season of calving	1171:1187	Dry period length (for cows of parity ≥2), milk yield of previous 305-d lactation (for cows of parity ≥2), age at calving, parity number, season of calving, time interval between calving and first colostrum milking, and milk yield were recorded.					
31500058	0	0	theme	enhanced	109:116	arg1	therapy					129:135	implantable enhanced anticancer therapy	97:135	implantable enhanced anticancer therapy	97:135	An ultrasound-controllable release system based on waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy.					
31500058	7	1	theme	chemotherapeutic	1640:1655	arg1	drugs					1657:1661	chemotherapeutic drugs	1640:1661	chemotherapeutic drugs	1640:1661	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	4	2	theme	favorable	922:930	arg1	cytocompatibility					932:948	favorable cytocompatibility	922:948	favorable cytocompatibility	922:948	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	7	3	theme	adjuvant	1387:1394	arg1	treatment					1402:1410	adjuvant local treatment	1387:1410	adjuvant local treatment	1387:1410	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	7	4	theme	long-term	1561:1569	arg1	efficiency					1583:1592	the higher and long-term therapeutic efficiency	1546:1592	the higher and long-term therapeutic efficiency	1546:1592	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	0	5	theme	implantable	97:107	arg1	therapy					129:135	implantable enhanced anticancer therapy	97:135	implantable enhanced anticancer therapy	97:135	An ultrasound-controllable release system based on waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy.					
31500058	7	6	theme	ultrasound-regulated	1467:1486	arg1	matrix					1497:1502	ultrasound-regulated membrane matrix	1467:1502	ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells	1467:1703	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	4	7	theme	resulting	863:871	arg1	films					883:887	the resulting composite films	859:887	the resulting composite films	859:887	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	3	8	theme	implantable	545:555	arg1	release-system					557:570	an ultrasound-controllable and implantable release-system	514:570	an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX)	514:725	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	3	9	theme	flexible	679:686	arg1	capacity					696:703	wide flexible loading capacity	674:703	wide flexible loading capacity for doxorubicin (DOX)	674:725	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	5	10	theme	drug-loading	1035:1046	arg1	membranes					1048:1056	the drug-loading membranes	1031:1056	the drug-loading membranes	1031:1056	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	4	11	theme	fine	899:902	arg1	biodegradability					904:919	fine biodegradability	899:919	fine biodegradability	899:919	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	4	12	from	WPU	801:803	arg1	chains					845:850	side chains	840:850	side chains of CS	840:856	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	2	13	theme	normal	314:319	arg1	tissues					321:327	normal tissues	314:327	normal tissues	314:327	New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs are still urgently required.					
31500058	4	14	from	bioactivity	809:819	arg1	chains					845:850	side chains	840:850	side chains of CS	840:856	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	7	15	theme	higher	1550:1555	arg1	efficiency					1583:1592	the higher and long-term therapeutic efficiency	1546:1592	the higher and long-term therapeutic efficiency	1546:1592	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	0	16	theme	anticancer	118:127	arg1	therapy					129:135	implantable enhanced anticancer therapy	97:135	implantable enhanced anticancer therapy	97:135	An ultrasound-controllable release system based on waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy.					
31500058	5	17	theme	no	1142:1143	arg1	release					1152:1158	no sudden release	1142:1158	no sudden release	1142:1158	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	5	18	theme	sudden	1145:1150	arg1	release					1152:1158	no sudden release	1142:1158	no sudden release	1142:1158	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	4	19	from	segment	790:796	arg1	chains					845:850	side chains	840:850	side chains of CS	840:856	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	4	19	from	segment	790:796	arg1	bioactivity					809:819	bioactivity	809:819	bioactivity	809:819	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	4	19	from	segment	790:796	arg1	WPU					801:803	WPU	801:803	WPU	801:803	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	4	19	from	segment	790:796	arg1	Benefiting					758:767	Benefiting	758:767	Benefiting	758:767	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	1	20	theme	cancer	198:203	arg1	therapy					205:211	cancer therapy	198:211	cancer therapy	198:211	Anti-relapse therapy after surgery plays a critical role in cancer therapy.					
31500058	3	21	theme	delivery	412:419	arg1	distribution					436:447	inadequate distribution	425:447	inadequate distribution of cytotoxic chemotherapeutic drugs	425:483	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	3	21	theme	delivery	412:419	arg1	problems					391:398	the problems	387:398	the problems of the poor delivery	387:419	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	4	22	theme	CS	855:856	arg1	chains					845:850	side chains	840:850	side chains of CS	840:856	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	2	23	theme	side	298:301	arg1	effects					303:309	toxic side effects	292:309	toxic side effects on normal tissues and organs	292:338	New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs are still urgently required.					
31500058	7	24	theme	normal	1692:1697	arg1	cells					1699:1703	normal cells	1692:1703	normal cells	1692:1703	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	3	25	used	utilized	577:584	arg2	release-system					557:570	an ultrasound-controllable and implantable release-system	514:570	an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX)	514:725	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	0	26	theme	ultrasound-controllable	3:25	arg1	system					35:40	An ultrasound-controllable release system	0:40	An ultrasound-controllable release system	0:40	An ultrasound-controllable release system based on waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy.					
31500058	3	27	theme	present	744:750	arg1	work					752:755	present work	744:755	present work	744:755	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	3	28	with	carrier	661:667	arg1	capacity					696:703	wide flexible loading capacity	674:703	wide flexible loading capacity for doxorubicin (DOX)	674:725	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	6	29	theme	uptake	1238:1243	arg1	assay					1245:1249	Cellular uptake assay	1229:1249	Cellular uptake assay	1229:1249	Cellular uptake assay and CCK 8 assay showed that the DOX can be released efficiently from the drug-loading matrix and taken up by tumor cells.					
31500058	7	30	theme	facile	1434:1439	arg1	means					1378:1382	a means	1376:1382	a means of adjuvant local treatment	1376:1410	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	7	30	theme	facile	1434:1439	arg1	approach					1441:1448	a facile approach	1432:1448	a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells	1432:1703	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	4	31	theme	composite	873:881	arg1	films					883:887	the resulting composite films	859:887	the resulting composite films	859:887	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	7	32	theme	treatment	1402:1410	arg1	means					1378:1382	a means	1376:1382	a means of adjuvant local treatment	1376:1410	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	7	32	theme	treatment	1402:1410	arg1	approach					1441:1448	a facile approach	1432:1448	a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells	1432:1703	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	5	33	theme	ultrasound-controlled	1199:1219	arg1	manner					1221:1226	an ultrasound-controlled manner	1196:1226	an ultrasound-controlled manner	1196:1226	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	4	34	theme	excellent	954:962	arg1	compatibility					970:982	excellent blood compatibility	954:982	excellent blood compatibility	954:982	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	6	35	theme	CCK	1255:1257	arg1	assay					1261:1265	CCK 8 assay	1255:1265	CCK 8 assay	1255:1265	Cellular uptake assay and CCK 8 assay showed that the DOX can be released efficiently from the drug-loading matrix and taken up by tumor cells.					
31500058	4	36	theme	hydrophilic	778:788	arg1	segment					790:796	the hydrophilic segment	774:796	the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS	774:856	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	4	37	theme	side	840:843	arg1	chains					845:850	side chains	840:850	side chains of CS	840:856	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	0	38	theme	release	27:33	arg1	system					35:40	An ultrasound-controllable release system	0:40	An ultrasound-controllable release system	0:40	An ultrasound-controllable release system based on waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy.					
31500058	7	39	theme	therapeutic	1571:1581	arg1	efficiency					1583:1592	the higher and long-term therapeutic efficiency	1546:1592	the higher and long-term therapeutic efficiency	1546:1592	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	7	40	theme	membrane	1488:1495	arg1	matrix					1497:1502	ultrasound-regulated membrane matrix	1467:1502	ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells	1467:1703	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	2	41	theme	New	214:216	arg1	strategies					218:227	New strategies	214:227	New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs	214:338	New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs are still urgently required.					
31500058	1	42	theme	Anti-relapse	138:149	arg1	therapy					151:157	Anti-relapse therapy	138:157	Anti-relapse therapy after surgery	138:171	Anti-relapse therapy after surgery plays a critical role in cancer therapy.					
31500058	5	43	theme	sustained	1075:1083	arg1	effect					1093:1098	a well sustained release effect	1068:1098	a well sustained release effect manifested in slow release, stability and no sudden release	1068:1158	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	3	44	theme	clinical	492:499	arg1	application					501:511	the clinical application	488:511	the clinical application	488:511	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	7	45	theme	drugs	1657:1661	arg1	efficiency					1626:1635	utilization efficiency	1614:1635	utilization efficiency of chemotherapeutic drugs	1614:1661	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	3	46	theme	loading	688:694	arg1	capacity					696:703	wide flexible loading capacity	674:703	wide flexible loading capacity for doxorubicin (DOX)	674:725	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	2	47	theme	tumor	265:269	arg1	cells					271:275	tumor cells	265:275	tumor cells	265:275	New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs are still urgently required.					
31500058	6	48	attach	released	1294:1301	arg1	matrix					1337:1342	the drug-loading matrix	1320:1342	the drug-loading matrix	1320:1342	Cellular uptake assay and CCK 8 assay showed that the DOX can be released efficiently from the drug-loading matrix and taken up by tumor cells.					
31500058	6	48	attach	released	1294:1301	arg2	DOX					1283:1285	the DOX	1279:1285	the DOX	1279:1285	Cellular uptake assay and CCK 8 assay showed that the DOX can be released efficiently from the drug-loading matrix and taken up by tumor cells.					
31500058	3	49	theme	drugs	479:483	arg1	distribution					436:447	inadequate distribution	425:447	inadequate distribution of cytotoxic chemotherapeutic drugs	425:483	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	3	49	theme	drugs	479:483	arg1	problems					391:398	the problems	387:398	the problems of the poor delivery	387:419	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	3	50	theme	inadequate	425:434	arg1	distribution					436:447	inadequate distribution	425:447	inadequate distribution of cytotoxic chemotherapeutic drugs	425:483	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	7	51	theme	efficiency	1626:1635	arg1	toxicity					1680:1687	the low toxicity	1672:1687	the low toxicity to normal cells	1672:1703	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	7	51	theme	efficiency	1626:1635	arg1	efficiency					1583:1592	the higher and long-term therapeutic efficiency	1546:1592	the higher and long-term therapeutic efficiency	1546:1592	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	7	51	theme	efficiency	1626:1635	arg1	improvement					1599:1609	improvement	1599:1609	improvement of utilization efficiency of chemotherapeutic drugs	1599:1661	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	4	52	theme	blood	964:968	arg1	compatibility					970:982	excellent blood compatibility	954:982	excellent blood compatibility	954:982	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	3	53	theme	composite	634:642	arg1	membrane					644:651	composite membrane	634:651	composite membrane	634:651	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	7	54	theme	utilization	1614:1624	arg1	efficiency					1626:1635	utilization efficiency	1614:1635	utilization efficiency of chemotherapeutic drugs	1614:1661	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	0	55	theme	polyurethane/chitosan	62:82	arg1	membrane					84:91	waterborne polyurethane/chitosan membrane	51:91	waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy	51:135	An ultrasound-controllable release system based on waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy.					
31500058	3	56	theme	cytotoxic	452:460	arg1	drugs					479:483	cytotoxic chemotherapeutic drugs	452:483	cytotoxic chemotherapeutic drugs	452:483	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	2	57	theme	drugs	256:260	arg1	delivery					244:251	the delivery	240:251	the delivery of drugs to tumor cells	240:275	New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs are still urgently required.					
31500058	4	58	from	chains	845:850	arg1	segment					790:796	the hydrophilic segment	774:796	the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS	774:856	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	0	59	theme	waterborne	51:60	arg1	membrane					84:91	waterborne polyurethane/chitosan membrane	51:91	waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy	51:135	An ultrasound-controllable release system based on waterborne polyurethane/chitosan membrane for implantable enhanced anticancer therapy.					
31500058	6	60	theme	tumor	1360:1364	arg1	cells					1366:1370	tumor cells	1360:1370	tumor cells	1360:1370	Cellular uptake assay and CCK 8 assay showed that the DOX can be released efficiently from the drug-loading matrix and taken up by tumor cells.					
31500058	3	61	theme	wide	674:677	arg1	capacity					696:703	wide flexible loading capacity	674:703	wide flexible loading capacity for doxorubicin (DOX)	674:725	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	5	62	theme	release	1085:1091	arg1	effect					1093:1098	a well sustained release effect	1068:1098	a well sustained release effect manifested in slow release, stability and no sudden release	1068:1158	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	3	63	theme	ultrasound-controllable	517:539	arg1	release-system					557:570	an ultrasound-controllable and implantable release-system	514:570	an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX)	514:725	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	2	64	from	effects	303:309	arg1	organs					333:338	organs	333:338	organs	333:338	New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs are still urgently required.					
31500058	2	64	from	effects	303:309	arg1	tissues					321:327	normal tissues	314:327	normal tissues	314:327	New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs are still urgently required.					
31500058	6	65	theme	Cellular	1229:1236	arg1	assay					1245:1249	Cellular uptake assay	1229:1249	Cellular uptake assay	1229:1249	Cellular uptake assay and CCK 8 assay showed that the DOX can be released efficiently from the drug-loading matrix and taken up by tumor cells.					
31500058	6	66	theme	drug-loading	1324:1335	arg1	matrix					1337:1342	the drug-loading matrix	1320:1342	the drug-loading matrix	1320:1342	Cellular uptake assay and CCK 8 assay showed that the DOX can be released efficiently from the drug-loading matrix and taken up by tumor cells.					
31500058	3	67	theme	drug	656:659	arg1	carrier					661:667	drug carrier	656:667	drug carrier with wide flexible loading capacity for doxorubicin (DOX)	656:725	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	2	68	theme	toxic	292:296	arg1	effects					303:309	toxic side effects	292:309	toxic side effects on normal tissues and organs	292:338	New strategies maximizing the delivery of drugs to tumor cells while reducing toxic side effects on normal tissues and organs are still urgently required.					
31500058	7	69	theme	local	1396:1400	arg1	treatment					1402:1410	adjuvant local treatment	1387:1410	adjuvant local treatment	1387:1410	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	3	70	dep	polyurethane	597:608	arg1	membrane					644:651	composite membrane	634:651	composite membrane	634:651	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	5	71	theme	in	989:990	arg1	studies					1006:1012	The in vitro release studies	985:1012	The in vitro release studies	985:1012	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	3	72	theme	waterborne	586:595	arg1	polyurethane					597:608	waterborne polyurethane	586:608	waterborne polyurethane (WPU)	586:614	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	3	72	theme	waterborne	586:595	arg1	WPU					611:613	WPU	611:613	WPU	611:613	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	5	73	theme	release	998:1004	arg1	studies					1006:1012	The in vitro release studies	985:1012	The in vitro release studies	985:1012	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	7	74	theme	low	1676:1678	arg1	toxicity					1680:1687	the low toxicity	1672:1687	the low toxicity to normal cells	1672:1703	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	5	75	theme	slow	1114:1117	arg1	release					1119:1125	slow release	1114:1125	slow release	1114:1125	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	4	76	theme	groups	830:835	arg1	bioactivity					809:819	bioactivity	809:819	bioactivity	809:819	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	4	76	theme	groups	830:835	arg1	WPU					801:803	WPU	801:803	WPU	801:803	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	5	77	dep	in	989:990	arg1	vitro					992:996	vitro	992:996	vitro	992:996	The in vitro release studies illustrated that the drug-loading membranes displayed a well sustained release effect manifested in slow release, stability and no sudden release, and the DOX was able to release in an ultrasound-controlled manner.					
31500058	3	78	theme	chemotherapeutic	462:477	arg1	drugs					479:483	cytotoxic chemotherapeutic drugs	452:483	cytotoxic chemotherapeutic drugs	452:483	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	3	79	theme	poor	407:410	arg1	delivery					412:419	the poor delivery	403:419	the poor delivery	403:419	In order to solve the problems of the poor delivery and inadequate distribution of cytotoxic chemotherapeutic drugs in the clinical application, an ultrasound-controllable and implantable release-system that utilized waterborne polyurethane (WPU) and chitosan (CS) composite membrane as drug carrier with wide flexible loading capacity for doxorubicin (DOX) was described in present work.					
31500058	7	80	theme	matrix	1497:1502	arg1	design					1457:1462	the design	1453:1462	the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells	1453:1703	As a means of adjuvant local treatment, this work provided a facile approach to the design of ultrasound-regulated membrane matrix that is highly beneficial not only due to the higher and long-term therapeutic efficiency, and improvement of utilization efficiency of chemotherapeutic drugs but also the low toxicity to normal cells.					
31500058	4	81	theme	amino	824:828	arg1	groups					830:835	amino groups	824:835	amino groups	824:835	Benefiting from the hydrophilic segment in WPU and bioactivity of amino groups on side chains of CS, the resulting composite films exhibited fine biodegradability, favorable cytocompatibility and excellent blood compatibility.					
31500058	1	82	theme	critical	181:188	arg1	role					190:193	a critical role	179:193	a critical role	179:193	Anti-relapse therapy after surgery plays a critical role in cancer therapy.					
31574117	9	0	theme	EIM	1538:1540	arg1	parameters					1542:1551	low frequency EIM parameters	1524:1551	low frequency EIM parameters	1524:1551	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	4	1	theme	%	674:674	arg1	λ-carrageenan					676:688	either 0.3% λ-carrageenan	664:688	either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone	664:736	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	9	2	theme	infiltrates	1599:1609	arg1	presence					1574:1581	the presence	1570:1581	the presence of inflammatory infiltrates	1570:1609	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	7	3	theme	PBS-treated	1030:1040	arg1	animals					1042:1048	PBS-treated animals	1030:1048	PBS-treated animals	1030:1048	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	6	4	from	reductions	979:988	arg1	reactance					1000:1008	reactance	1000:1008	reactance	1000:1008	Parallel alterations in both 5 and 50 kHz EIM values were identified at 4 and 24 hours, including reductions in phase, reactance, and resistance.					
31574117	6	4	from	reductions	979:988	arg1	resistance					1015:1024	resistance	1015:1024	resistance	1015:1024	Parallel alterations in both 5 and 50 kHz EIM values were identified at 4 and 24 hours, including reductions in phase, reactance, and resistance.					
31574117	6	4	from	reductions	979:988	arg1	phase					993:997	phase	993:997	phase	993:997	Parallel alterations in both 5 and 50 kHz EIM values were identified at 4 and 24 hours, including reductions in phase, reactance, and resistance.					
31574117	4	5	theme	0.3	671:673	arg1	%					674:674	%	674:674	%	674:674	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	6	theme	mice	557:560	arg1	total					545:549	A total	543:549	A total of 91 mice	543:560	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	9	7	dep	presence	1683:1690	arg1	the					1679:1681	the	1679:1681	the	1679:1681	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	7	8	dep	PBS	1311:1313	arg1	p<0.001					1315:1321	p<0.001	1315:1321	p<0.001	1315:1321	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	3	9	theme	λ-carrageenan	528:540	arg1	injection					515:523	the localized intramuscular injection	487:523	the localized intramuscular injection of λ-carrageenan	487:540	In this study, we sought to assess EIM sensitivity to experimental inflammation induced by the localized intramuscular injection of λ-carrageenan.					
31574117	4	10	theme	kHz	579:581	arg1	measurements					587:598	1-1000 kHz EIM measurements	572:598	1-1000 kHz EIM measurements of gastrocnemius	572:615	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	8	11	theme	basophilic	1357:1366	arg1	area					1368:1371	basophilic area	1357:1371	basophilic area observed in muscle sections by histology	1357:1412	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31574117	5	12	theme	quantitative	822:833	arg1	assessment					835:844	quantitative assessment	822:844	quantitative assessment of muscle histology	822:864	Animals were then remeasured with EIM at 4, 24, 48, or 72 hours and euthanized and quantitative assessment of muscle histology was performed.					
31574117	4	13	from	injection	651:659	arg1	PBS					720:722	PBS	720:722	PBS	720:722	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	13	from	injection	651:659	arg1	PBS					728:730	PBS	728:730	PBS	728:730	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	13	from	injection	651:659	arg1	saline					712:717	phosphate-buffered saline	693:717	phosphate-buffered saline (PBS)	693:723	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	14	theme	phosphate-buffered	693:710	arg1	PBS					720:722	PBS	720:722	PBS	720:722	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	14	theme	phosphate-buffered	693:710	arg1	saline					712:717	phosphate-buffered saline	693:717	phosphate-buffered saline (PBS)	693:723	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	15	from	PBS	728:730	arg1	injection					651:659	injection	651:659	injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone	651:736	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	2	16	theme	pure	377:380	arg1	inflammation					382:393	pure inflammation	377:393	pure inflammation	377:393	However, it has been minimally studied in pure inflammation.					
31574117	7	17	theme	substantial	1095:1105	arg1	reductions					1107:1116	substantial reductions	1095:1116	substantial reductions in phase and reactance in 5 kHz EIM values	1095:1159	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	7	18	theme	phase	1207:1211	arg1	post-injection					1222:1235	phase 72 hours post-injection	1207:1235	phase 72 hours post-injection	1207:1235	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	0	19	theme	impedance	11:19	arg1	myography					21:29	Electrical impedance myography	0:29	Electrical impedance myography for the detection of muscle inflammation induced by λ-carrageenan	0:95	Electrical impedance myography for the detection of muscle inflammation induced by λ-carrageenan.					
31574117	8	20	theme	area	1368:1371	arg1	degree					1347:1352	The degree	1343:1352	The degree of basophilic area observed in muscle sections by histology	1343:1412	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31574117	8	21	theme	muscle	1385:1390	arg1	sections					1392:1399	muscle sections	1385:1399	muscle sections	1385:1399	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31574117	9	22	theme	muscle	1706:1711	arg1	inflammation					1713:1724	muscle inflammation	1706:1724	muscle inflammation	1706:1724	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	1	23	theme	muscle	172:177	arg1	health					179:184	muscle health	172:184	muscle health	172:184	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	0	24	theme	Electrical	0:9	arg1	myography					21:29	Electrical impedance myography	0:29	Electrical impedance myography for the detection of muscle inflammation induced by λ-carrageenan	0:95	Electrical impedance myography for the detection of muscle inflammation induced by λ-carrageenan.					
31574117	7	25	dep	=	1326:1326	arg1	i.e.					1191:1194	i.e.	1191:1194	i.e.	1191:1194	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	7	26	dep	normalized	1063:1072	arg1	whereas					1087:1093	whereas	1087:1093	whereas	1087:1093	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	1	27	theme	Electrical	98:107	arg1	technique					140:148	a technique	138:148	a technique for the assessment of muscle health and composition	138:200	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	27	theme	Electrical	98:107	arg1	EIM					130:132	EIM	130:132	EIM	130:132	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	27	theme	Electrical	98:107	arg1	myography					119:127	Electrical impedance myography	98:127	Electrical impedance myography (EIM)	98:133	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	9	28	theme	inflammation	1713:1724	arg1	extent					1696:1701	extent	1696:1701	extent	1696:1701	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	9	28	theme	inflammation	1713:1724	arg1	presence					1683:1690	presence	1683:1690	presence	1683:1690	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	1	29	theme	health	179:184	arg1	assessment					158:167	the assessment	154:167	the assessment of muscle health and composition	154:200	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	30	theme	muscle	253:258	arg1	pathologies					260:270	muscle pathologies	253:270	muscle pathologies including neurogenic atrophy and connective tissue deposition	253:332	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	30	theme	muscle	253:258	arg1	atrophy					293:299	neurogenic atrophy	282:299	neurogenic atrophy	282:299	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	30	theme	muscle	253:258	arg1	deposition					323:332	connective tissue deposition	305:332	connective tissue deposition	305:332	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	31	theme	impedance	109:117	arg1	technique					140:148	a technique	138:148	a technique for the assessment of muscle health and composition	138:200	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	31	theme	impedance	109:117	arg1	EIM					130:132	EIM	130:132	EIM	130:132	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	31	theme	impedance	109:117	arg1	myography					119:127	Electrical impedance myography	98:127	Electrical impedance myography (EIM)	98:133	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	3	32	theme	EIM	431:433	arg1	sensitivity					435:445	EIM sensitivity	431:445	EIM sensitivity to experimental inflammation induced by the localized intramuscular injection of λ-carrageenan	431:540	In this study, we sought to assess EIM sensitivity to experimental inflammation induced by the localized intramuscular injection of λ-carrageenan.					
31574117	1	33	theme	pathologies	260:270	arg1	pathologies					260:270	muscle pathologies	253:270	muscle pathologies including neurogenic atrophy and connective tissue deposition	253:332	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	33	theme	pathologies	260:270	arg1	atrophy					293:299	neurogenic atrophy	282:299	neurogenic atrophy	282:299	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	33	theme	pathologies	260:270	arg1	deposition					323:332	connective tissue deposition	305:332	connective tissue deposition	305:332	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	1	33	theme	pathologies	260:270	arg1	variety					242:248	a variety	240:248	a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition	240:332	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	7	34	theme	kHz	1146:1148	arg1	values					1154:1159	5 kHz EIM values	1144:1159	5 kHz EIM values	1144:1159	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	1	35	theme	composition	190:200	arg1	assessment					158:167	the assessment	154:167	the assessment of muscle health and composition	154:200	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	5	36	theme	muscle	849:854	arg1	histology					856:864	muscle histology	849:864	muscle histology	849:864	Animals were then remeasured with EIM at 4, 24, 48, or 72 hours and euthanized and quantitative assessment of muscle histology was performed.					
31574117	7	37	theme	EIM	1150:1152	arg1	values					1154:1159	5 kHz EIM values	1144:1159	5 kHz EIM values	1144:1159	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	9	38	contain	have	1616:1619	arg2	potential					1625:1633	the potential	1621:1633	the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy	1621:1752	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	9	38	contain	have	1616:1619	arg1	Changes					1513:1519	Changes	1513:1519	Changes in low frequency EIM parameters	1513:1551	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	8	39	located	observed	1373:1380	arg1	sections					1392:1399	muscle sections	1385:1399	muscle sections	1385:1399	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31574117	8	39	located	observed	1373:1380	arg2	area					1368:1371	basophilic area	1357:1371	basophilic area observed in muscle sections by histology	1357:1412	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31574117	1	40	theme	neurogenic	282:291	arg1	atrophy					293:299	neurogenic atrophy	282:299	neurogenic atrophy	282:299	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	6	41	theme	EIM	923:925	arg1	values					927:932	both 5 and 50 kHz EIM values	905:932	values	927:932	Parallel alterations in both 5 and 50 kHz EIM values were identified at 4 and 24 hours, including reductions in phase, reactance, and resistance.					
31574117	4	42	dep	underwent	562:570	arg1	followed					639:646	followed	639:646	followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone	639:736	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	8	43	theme	change	1448:1453	arg1	degree					1432:1437	the degree	1428:1437	the degree of phase change	1428:1453	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31574117	8	44	dep	=	1502:1502	arg1	Rspearman					1481:1489	Rspearman	1481:1489	Rspearman	1481:1489	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31574117	4	45	theme	needle	625:630	arg1	array					632:636	a needle array	623:636	a needle array	623:636	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	0	46	theme	muscle	52:57	arg1	inflammation					59:70	muscle inflammation	52:70	muscle inflammation induced by λ-carrageenan	52:95	Electrical impedance myography for the detection of muscle inflammation induced by λ-carrageenan.					
31574117	6	47	theme	Parallel	881:888	arg1	alterations					890:900	Parallel alterations	881:900	Parallel alterations in both 5 and 50 kHz EIM values	881:932	Parallel alterations in both 5 and 50 kHz EIM values were identified at 4 and 24 hours, including reductions in phase, reactance, and resistance.					
31574117	9	48	theme	frequency	1528:1536	arg1	parameters					1542:1551	low frequency EIM parameters	1524:1551	low frequency EIM parameters	1524:1551	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	9	49	theme	low	1524:1526	arg1	parameters					1542:1551	low frequency EIM parameters	1524:1551	low frequency EIM parameters	1524:1551	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	9	50	from	Changes	1513:1519	arg1	parameters					1542:1551	low frequency EIM parameters	1524:1551	low frequency EIM parameters	1524:1551	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	4	51	theme	λ-carrageenan	676:688	arg1	injection					651:659	injection	651:659	injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone	651:736	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	52	from	saline	712:717	arg1	injection					651:659	injection	651:659	injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone	651:736	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	1	53	theme	connective	305:314	arg1	deposition					323:332	connective tissue deposition	305:332	connective tissue deposition	305:332	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	6	54	from	alterations	890:900	arg1	values					927:932	both 5 and 50 kHz EIM values	905:932	values	927:932	Parallel alterations in both 5 and 50 kHz EIM values were identified at 4 and 24 hours, including reductions in phase, reactance, and resistance.					
31574117	6	54	from	alterations	890:900	arg1	5					910:910	5	910:910	5	910:910	Parallel alterations in both 5 and 50 kHz EIM values were identified at 4 and 24 hours, including reductions in phase, reactance, and resistance.					
31574117	7	55	theme	post-injection	1222:1235	arg1	values					1197:1202	values	1197:1202	values of phase 72 hours post-injection	1197:1235	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	5	56	theme	histology	856:864	arg1	assessment					835:844	quantitative assessment	822:844	quantitative assessment of muscle histology	822:864	Animals were then remeasured with EIM at 4, 24, 48, or 72 hours and euthanized and quantitative assessment of muscle histology was performed.					
31574117	6	57	theme	kHz	919:921	arg1	values					927:932	both 5 and 50 kHz EIM values	905:932	values	927:932	Parallel alterations in both 5 and 50 kHz EIM values were identified at 4 and 24 hours, including reductions in phase, reactance, and resistance.					
31574117	7	58	from	reductions	1107:1116	arg1	values					1154:1159	5 kHz EIM values	1144:1159	5 kHz EIM values	1144:1159	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	7	58	from	reductions	1107:1116	arg1	reactance					1131:1139	reactance	1131:1139	reactance	1131:1139	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	7	58	from	reductions	1107:1116	arg1	phase					1121:1125	phase	1121:1125	phase	1121:1125	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	9	59	theme	inflammatory	1586:1597	arg1	infiltrates					1599:1609	inflammatory infiltrates	1586:1609	inflammatory infiltrates	1586:1609	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	8	60	dep	=	1491:1491	arg1	-0.51					1493:1497	-0.51	1493:1497	-0.51	1493:1497	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31574117	3	61	theme	experimental	450:461	arg1	inflammation					463:474	experimental inflammation	450:474	experimental inflammation induced by the localized intramuscular injection of λ-carrageenan	450:540	In this study, we sought to assess EIM sensitivity to experimental inflammation induced by the localized intramuscular injection of λ-carrageenan.					
31574117	9	62	theme	simple	1651:1656	arg1	means					1658:1662	a simple means	1649:1662	a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy	1649:1752	Changes in low frequency EIM parameters are sensitive to the presence of inflammatory infiltrates, and have the potential of serving as a simple means of quantifying the presence and extent of muscle inflammation without the need for biopsy.					
31574117	4	63	theme	EIM	583:585	arg1	measurements					587:598	1-1000 kHz EIM measurements	572:598	1-1000 kHz EIM measurements of gastrocnemius	572:615	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	3	64	theme	localized	491:499	arg1	injection					515:523	the localized intramuscular injection	487:523	the localized intramuscular injection of λ-carrageenan	487:540	In this study, we sought to assess EIM sensitivity to experimental inflammation induced by the localized intramuscular injection of λ-carrageenan.					
31574117	4	65	from	λ-carrageenan	676:688	arg1	PBS					720:722	PBS	720:722	PBS	720:722	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	65	from	λ-carrageenan	676:688	arg1	PBS					728:730	PBS	728:730	PBS	728:730	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	65	from	λ-carrageenan	676:688	arg1	saline					712:717	phosphate-buffered saline	693:717	phosphate-buffered saline (PBS)	693:723	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	4	66	theme	gastrocnemius	603:615	arg1	measurements					587:598	1-1000 kHz EIM measurements	572:598	1-1000 kHz EIM measurements of gastrocnemius	572:615	A total of 91 mice underwent 1-1000 kHz EIM measurements of gastrocnemius using a needle array, followed by injection of either 0.3% λ-carrageenan in phosphate-buffered saline (PBS) or PBS alone.					
31574117	0	67	theme	inflammation	59:70	arg1	detection					39:47	the detection	35:47	the detection of muscle inflammation induced by λ-carrageenan	35:95	Electrical impedance myography for the detection of muscle inflammation induced by λ-carrageenan.					
31574117	1	68	theme	tissue	316:321	arg1	deposition					323:332	connective tissue deposition	305:332	connective tissue deposition	305:332	Electrical impedance myography (EIM) is a technique for the assessment of muscle health and composition and has been shown to be sensitive to a variety of muscle pathologies including neurogenic atrophy and connective tissue deposition.					
31574117	7	69	theme	hours	1216:1220	arg1	post-injection					1222:1235	phase 72 hours post-injection	1207:1235	phase 72 hours post-injection	1207:1235	In PBS-treated animals these values normalized by 48 hours, whereas substantial reductions in phase and reactance in 5 kHz EIM values persisted at 48 and 72 hours (i.e., values of phase 72 hours post-injection were 6.51 ± 0.40 degrees for λ-carrageenan versus 8.44 ± 0.35 degrees for PBS p<0.001, n = 11 per group).					
31574117	3	70	theme	intramuscular	501:513	arg1	injection					515:523	the localized intramuscular injection	487:523	the localized intramuscular injection of λ-carrageenan	487:540	In this study, we sought to assess EIM sensitivity to experimental inflammation induced by the localized intramuscular injection of λ-carrageenan.					
31574117	8	71	theme	time	1468:1471	arg1	points					1473:1478	these two time points	1458:1478	these two time points	1458:1478	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31574117	8	72	theme	phase	1442:1446	arg1	change					1448:1453	phase change	1442:1453	phase change	1442:1453	The degree of basophilic area observed in muscle sections by histology correlated to the degree of phase change at these two time points (Rspearman = -0.51, p = 0.0029).					
31740324	14	0	dep	reached	1408:1414	arg1	recycling					1363:1371	recycling	1363:1371	recycling	1363:1371	YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1).					
31740324	14	0	dep	reached	1408:1414	arg1	1.50 μg/mL/h					1416:1427	1.50 μg/mL/h	1416:1427	reached 1.50 μg/mL/h in the sixth cycle	1408:1446	YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1).					
31740324	14	0	dep	reached	1408:1414	arg1	reusing					1377:1383	reusing	1377:1383	reusing	1377:1383	YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1).					
31740324	10	1	theme	immobilization	981:994	arg1	conditions					996:1005	the optimal immobilization conditions	969:1005	the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃	969:1075	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	5	2	theme	vibrating	661:669	arg1	VSM					692:694	VSM	692:694	VSM	692:694	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	2	theme	vibrating	661:669	arg1	magnetometry					678:689	vibrating sample magnetometry	661:689	vibrating sample magnetometry (VSM)	661:695	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	17	3	theme	environment	1809:1819	arg1	treatment					1777:1785	treatment	1777:1785	treatment of MC-LR contaminated environment	1777:1819	YF1 can be potentially used for treatment of MC-LR contaminated environment.					
31740324	8	4	theme	Fe3O4	874:878	arg1	sp					897:898	Fe3O4@CTS/Sphingopyxis sp	874:898	Fe3O4@CTS/Sphingopyxis sp	874:898	And the MC-LR-degrading capabilities of Fe3O4@CTS/Sphingopyxis sp.					
31740324	11	5	theme	CTS/Sphingopyxis	1088:1103	arg1	sp					1105:1106	The Fe3O4@CTS/Sphingopyxis sp	1078:1106	The Fe3O4@CTS/Sphingopyxis sp.	1078:1107	The Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	6	theme	sample	671:676	arg1	VSM					692:694	VSM	692:694	VSM	692:694	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	6	theme	sample	671:676	arg1	magnetometry					678:689	vibrating sample magnetometry	661:689	vibrating sample magnetometry (VSM)	661:695	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	7	theme	emission	613:620	arg1	SEM					652:654	SEM	652:654	SEM	652:654	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	7	theme	emission	613:620	arg1	microscopy					640:649	Field emission scanning electron microscopy	607:649	Field emission scanning electron microscopy (SEM)	607:655	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	10	8	contain	containing	1007:1016	arg2	immobilization					1046:1059	immobilization	1046:1059	immobilization for 4 h	1046:1067	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	10	8	contain	containing	1007:1016	arg2	glutaraldehyde					1030:1043	1.0 % (v/v) glutaraldehyde	1018:1043	1.0 % (v/v) glutaraldehyde	1018:1043	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	10	8	contain	containing	1007:1016	arg1	conditions					996:1005	the optimal immobilization conditions	969:1005	the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃	969:1075	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	8	9	theme	sp	897:898	arg1	capabilities					858:869	the MC-LR-degrading capabilities	838:869	the MC-LR-degrading capabilities of Fe3O4@CTS/Sphingopyxis sp	838:898	And the MC-LR-degrading capabilities of Fe3O4@CTS/Sphingopyxis sp.					
31740324	3	10	theme	bio-functionalized	348:365	arg1	composite					367:375	a novel bio-functionalized composite	340:375	a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp	340:488	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	4	11	theme	@	548:548	arg1	particles					532:540	chitosan-grafted Fe3O4 magnetic particles	500:540	chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS)	500:552	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	4	11	theme	@	548:548	arg1	CTS					549:551	Fe3O4@CTS	543:551	Fe3O4@CTS	543:551	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	5	12	theme	scanning	622:629	arg1	SEM					652:654	SEM	652:654	SEM	652:654	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	12	theme	scanning	622:629	arg1	microscopy					640:649	Field emission scanning electron microscopy	607:649	Field emission scanning electron microscopy (SEM)	607:655	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	8	13	theme	CTS/Sphingopyxis	880:895	arg1	sp					897:898	Fe3O4@CTS/Sphingopyxis sp	874:898	Fe3O4@CTS/Sphingopyxis sp	874:898	And the MC-LR-degrading capabilities of Fe3O4@CTS/Sphingopyxis sp.					
31740324	15	14	dep	intermediates	1516:1528	arg1	intermediates					1516:1528	Two intermediates	1512:1528	Two intermediates (tetrapeptide and Adda)	1512:1552	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	14	dep	intermediates	1516:1528	arg1	Adda					1548:1551	Adda	1548:1551	Adda	1548:1551	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	14	dep	intermediates	1516:1528	arg1	tetrapeptide					1531:1542	tetrapeptide	1531:1542	tetrapeptide	1531:1542	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	4	15	theme	Fe3O4	517:521	arg1	particles					532:540	chitosan-grafted Fe3O4 magnetic particles	500:540	chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS)	500:552	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	4	15	theme	Fe3O4	517:521	arg1	CTS					549:551	Fe3O4@CTS	543:551	Fe3O4@CTS	543:551	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	5	16	theme	electron	631:638	arg1	SEM					652:654	SEM	652:654	SEM	652:654	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	16	theme	electron	631:638	arg1	microscopy					640:649	Field emission scanning electron microscopy	607:649	Field emission scanning electron microscopy (SEM)	607:655	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	17	17	used	used	1768:1771	arg2	YF1					1745:1747	YF1	1745:1747	YF1	1745:1747	YF1 can be potentially used for treatment of MC-LR contaminated environment.					
31740324	16	18	theme	Fe3O4	1718:1722	arg1	sp					1741:1742	Fe3O4@CTS/Sphingopyxis sp	1718:1742	Fe3O4@CTS/Sphingopyxis sp	1718:1742	These results revealed that Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	19	theme	Fe3O4	756:760	arg1	sp					779:780	Fe3O4@CTS/Sphingopyxis sp	756:780	Fe3O4@CTS/Sphingopyxis sp	756:780	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	4	20	theme	chitosan-grafted	500:515	arg1	particles					532:540	chitosan-grafted Fe3O4 magnetic particles	500:540	chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS)	500:552	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	4	20	theme	chitosan-grafted	500:515	arg1	CTS					549:551	Fe3O4@CTS	543:551	Fe3O4@CTS	543:551	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	2	21	theme	health	273:278	arg1	attention					280:288	a wide public health attention	259:288	a wide public health attention	259:288	The microcystin-LR (MC-LR) produced due to harmful cyanobacterial blooms have brought great harm to human and aquatic organisms, attracting a wide public health attention.					
31740324	3	22	theme	immobilizing	461:472	arg1	sp					487:488	covalent immobilizing Sphingopyxis sp	452:488	covalent immobilizing Sphingopyxis sp	452:488	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	3	23	theme	novel	342:346	arg1	composite					367:375	a novel bio-functionalized composite	340:375	a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp	340:488	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	12	24	theme	degradation	1134:1144	arg1	performance					1146:1156	an attractive degradation performance	1120:1156	an attractive degradation performance which possesed a wide torlerance to pH (6.0-9.0) and temperature (25-35 ℃)	1120:1231	YF1 showed an attractive degradation performance which possesed a wide torlerance to pH (6.0-9.0) and temperature (25-35 ℃).					
31740324	14	25	theme	sixth	1436:1440	arg1	cycle					1442:1446	the sixth cycle	1432:1446	the sixth cycle	1432:1446	YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1).					
31740324	2	26	theme	public	266:271	arg1	attention					280:288	a wide public health attention	259:288	a wide public health attention	259:288	The microcystin-LR (MC-LR) produced due to harmful cyanobacterial blooms have brought great harm to human and aquatic organisms, attracting a wide public health attention.					
31740324	3	27	theme	Sphingopyxis	474:485	arg1	sp					487:488	covalent immobilizing Sphingopyxis sp	452:488	covalent immobilizing Sphingopyxis sp	452:488	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	3	28	theme	high-efficient	385:398	arg1	biodegradation					416:429	the high-efficient and sustainable biodegradation	381:429	the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp	381:488	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	4	29	theme	magnetic	523:530	arg1	particles					532:540	chitosan-grafted Fe3O4 magnetic particles	500:540	chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS)	500:552	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	4	29	theme	magnetic	523:530	arg1	CTS					549:551	Fe3O4@CTS	543:551	Fe3O4@CTS	543:551	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	14	30	dep	exhibited	1294:1302	arg1	increased					1318:1326	increased	1318:1326	exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1)	1294:1509	YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1).					
31740324	13	31	theme	@	1268:1268	arg1	sp					1286:1287	the Fe3O4@CTS/Sphingopyxis sp	1259:1287	the Fe3O4@CTS/Sphingopyxis sp	1259:1287	More interesting is that the Fe3O4@CTS/Sphingopyxis sp.					
31740324	1	32	theme	Fe3O4	103:107	arg1	chitosan					109:116	Fe3O4@chitosan	103:116	Fe3O4@chitosan	103:116	YF1 immobilized Fe3O4@chitosan.					
31740324	9	33	theme	various	925:931	arg1	conditions					933:942	various conditions	925:942	various conditions	925:942	YF1 were assessed under various conditions.					
31740324	11	34	theme	Fe3O4	1082:1086	arg1	sp					1105:1106	The Fe3O4@CTS/Sphingopyxis sp	1078:1106	The Fe3O4@CTS/Sphingopyxis sp.	1078:1107	The Fe3O4@CTS/Sphingopyxis sp.					
31740324	0	35	theme	High-efficient	0:13	arg1	biodegradation					31:44	High-efficient and sustainable biodegradation	0:44	High-efficient and sustainable biodegradation of microcystin-LR	0:62	High-efficient and sustainable biodegradation of microcystin-LR using Sphingopyxis sp.					
31740324	12	36	theme	attractive	1123:1132	arg1	performance					1146:1156	an attractive degradation performance	1120:1156	an attractive degradation performance which possesed a wide torlerance to pH (6.0-9.0) and temperature (25-35 ℃)	1120:1231	YF1 showed an attractive degradation performance which possesed a wide torlerance to pH (6.0-9.0) and temperature (25-35 ℃).					
31740324	13	37	theme	CTS/Sphingopyxis	1269:1284	arg1	sp					1286:1287	the Fe3O4@CTS/Sphingopyxis sp	1259:1287	the Fe3O4@CTS/Sphingopyxis sp	1259:1287	More interesting is that the Fe3O4@CTS/Sphingopyxis sp.					
31740324	1	38	theme	@	108:108	arg1	chitosan					109:116	Fe3O4@chitosan	103:116	Fe3O4@chitosan	103:116	YF1 immobilized Fe3O4@chitosan.					
31740324	3	39	theme	covalent	452:459	arg1	sp					487:488	covalent immobilizing Sphingopyxis sp	452:488	covalent immobilizing Sphingopyxis sp	452:488	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	16	40	theme	CTS/Sphingopyxis	1724:1739	arg1	sp					1741:1742	Fe3O4@CTS/Sphingopyxis sp	1718:1742	Fe3O4@CTS/Sphingopyxis sp	1718:1742	These results revealed that Fe3O4@CTS/Sphingopyxis sp.					
31740324	14	41	theme	MC-LR-degrading	1328:1342	arg1	capabilities					1344:1355	MC-LR-degrading capabilities	1328:1355	MC-LR-degrading capabilities	1328:1355	YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1).					
31740324	5	42	theme	Field	607:611	arg1	SEM					652:654	SEM	652:654	SEM	652:654	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	42	theme	Field	607:611	arg1	microscopy					640:649	Field emission scanning electron microscopy	607:649	Field emission scanning electron microscopy (SEM)	607:655	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	10	43	theme	optimal	973:979	arg1	conditions					996:1005	the optimal immobilization conditions	969:1005	the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃	969:1075	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	5	44	dep	Fourier	559:565	arg1	transform					567:575	transform	567:575	transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM)	567:695	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	0	45	theme	sustainable	19:29	arg1	biodegradation					31:44	High-efficient and sustainable biodegradation	0:44	High-efficient and sustainable biodegradation of microcystin-LR	0:62	High-efficient and sustainable biodegradation of microcystin-LR using Sphingopyxis sp.					
31740324	13	46	dep	is	1251:1252	arg1	sp					1286:1287	the Fe3O4@CTS/Sphingopyxis sp	1259:1287	the Fe3O4@CTS/Sphingopyxis sp	1259:1287	More interesting is that the Fe3O4@CTS/Sphingopyxis sp.					
31740324	10	47	dep	showed	957:962	arg1	conditions					996:1005	the optimal immobilization conditions	969:1005	the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃	969:1075	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	14	48	theme	degradation	1391:1401	arg1	rate					1403:1406	degradation rate	1391:1406	degradation rate	1391:1406	YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1).					
31740324	0	49	theme	microcystin-LR	49:62	arg1	biodegradation					31:44	High-efficient and sustainable biodegradation	0:44	High-efficient and sustainable biodegradation of microcystin-LR	0:62	High-efficient and sustainable biodegradation of microcystin-LR using Sphingopyxis sp.					
31740324	11	50	theme	@	1087:1087	arg1	sp					1105:1106	The Fe3O4@CTS/Sphingopyxis sp	1078:1106	The Fe3O4@CTS/Sphingopyxis sp.	1078:1107	The Fe3O4@CTS/Sphingopyxis sp.					
31740324	15	51	theme	degradation	1564:1574	arg1	mlrC					1606:1609	mlrC	1606:1609	mlrC	1606:1609	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	51	theme	degradation	1564:1574	arg1	mlrB					1597:1600	mlrB	1597:1600	mlrB	1597:1600	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	51	theme	degradation	1564:1574	arg1	mlrA					1591:1594	mlrA	1591:1594	mlrA	1591:1594	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	51	theme	degradation	1564:1574	arg1	genes					1584:1588	three degradation related genes	1558:1588	three degradation related genes (mlrA, mlrB and mlrC)	1558:1610	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	3	52	theme	microcystin-LR	434:447	arg1	biodegradation					416:429	the high-efficient and sustainable biodegradation	381:429	the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp	381:488	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	7	53	theme	immobilization	792:805	arg1	conditions					807:816	The immobilization conditions	788:816	The immobilization conditions	788:816	The immobilization conditions were optimized.					
31740324	5	54	theme	structural	731:740	arg1	properties					742:751	the structural properties	727:751	the structural properties of Fe3O4@CTS/Sphingopyxis sp	727:780	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	2	55	theme	cyanobacterial	170:183	arg1	blooms					185:190	harmful cyanobacterial blooms	162:190	harmful cyanobacterial blooms	162:190	The microcystin-LR (MC-LR) produced due to harmful cyanobacterial blooms have brought great harm to human and aquatic organisms, attracting a wide public health attention.					
31740324	2	56	theme	aquatic	229:235	arg1	organisms					237:245	aquatic organisms	229:245	aquatic organisms	229:245	The microcystin-LR (MC-LR) produced due to harmful cyanobacterial blooms have brought great harm to human and aquatic organisms, attracting a wide public health attention.					
31740324	5	57	theme	@	761:761	arg1	sp					779:780	Fe3O4@CTS/Sphingopyxis sp	756:780	Fe3O4@CTS/Sphingopyxis sp	756:780	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	0	58	theme	Sphingopyxis	70:81	arg1	sp					83:84	Sphingopyxis sp	70:84	Sphingopyxis sp	70:84	High-efficient and sustainable biodegradation of microcystin-LR using Sphingopyxis sp.					
31740324	17	59	theme	contaminated	1796:1807	arg1	environment					1809:1819	MC-LR contaminated environment	1790:1819	MC-LR contaminated environment	1790:1819	YF1 can be potentially used for treatment of MC-LR contaminated environment.					
31740324	2	60	theme	harmful	162:168	arg1	blooms					185:190	harmful cyanobacterial blooms	162:190	harmful cyanobacterial blooms	162:190	The microcystin-LR (MC-LR) produced due to harmful cyanobacterial blooms have brought great harm to human and aquatic organisms, attracting a wide public health attention.					
31740324	10	61	theme	1.0	1018:1020	arg1	%					1022:1022	%	1022:1022	%	1022:1022	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	5	62	theme	infrared	577:584	arg1	FTIR					600:603	FTIR	600:603	FTIR	600:603	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	5	62	theme	infrared	577:584	arg1	spectroscopy					586:597	infrared spectroscopy	577:597	infrared spectroscopy (FTIR)	577:604	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	17	63	theme	MC-LR	1790:1794	arg1	environment					1809:1819	MC-LR contaminated environment	1790:1819	MC-LR contaminated environment	1790:1819	YF1 can be potentially used for treatment of MC-LR contaminated environment.					
31740324	15	64	theme	related	1576:1582	arg1	mlrC					1606:1609	mlrC	1606:1609	mlrC	1606:1609	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	64	theme	related	1576:1582	arg1	mlrB					1597:1600	mlrB	1597:1600	mlrB	1597:1600	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	64	theme	related	1576:1582	arg1	mlrA					1591:1594	mlrA	1591:1594	mlrA	1591:1594	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	64	theme	related	1576:1582	arg1	genes					1584:1588	three degradation related genes	1558:1588	three degradation related genes (mlrA, mlrB and mlrC)	1558:1610	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	3	65	theme	MC-LR	304:308	arg1	contamination					310:322	MC-LR contamination	304:322	MC-LR contamination	304:322	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	8	66	theme	MC-LR-degrading	842:856	arg1	capabilities					858:869	the MC-LR-degrading capabilities	838:869	the MC-LR-degrading capabilities of Fe3O4@CTS/Sphingopyxis sp	838:898	And the MC-LR-degrading capabilities of Fe3O4@CTS/Sphingopyxis sp.					
31740324	13	67	theme	Fe3O4	1263:1267	arg1	sp					1286:1287	the Fe3O4@CTS/Sphingopyxis sp	1259:1287	the Fe3O4@CTS/Sphingopyxis sp	1259:1287	More interesting is that the Fe3O4@CTS/Sphingopyxis sp.					
31740324	2	68	theme	wide	261:264	arg1	attention					280:288	a wide public health attention	259:288	a wide public health attention	259:288	The microcystin-LR (MC-LR) produced due to harmful cyanobacterial blooms have brought great harm to human and aquatic organisms, attracting a wide public health attention.					
31740324	5	69	theme	sp	779:780	arg1	properties					742:751	the structural properties	727:751	the structural properties of Fe3O4@CTS/Sphingopyxis sp	727:780	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	16	70	theme	@	1723:1723	arg1	sp					1741:1742	Fe3O4@CTS/Sphingopyxis sp	1718:1742	Fe3O4@CTS/Sphingopyxis sp	1718:1742	These results revealed that Fe3O4@CTS/Sphingopyxis sp.					
31740324	10	71	theme	%	1022:1022	arg1	immobilization					1046:1059	immobilization	1046:1059	immobilization for 4 h	1046:1067	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	10	71	theme	%	1022:1022	arg1	glutaraldehyde					1030:1043	1.0 % (v/v) glutaraldehyde	1018:1043	1.0 % (v/v) glutaraldehyde	1018:1043	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	2	72	theme	great	205:209	arg1	harm					211:214	great harm	205:214	great harm	205:214	The microcystin-LR (MC-LR) produced due to harmful cyanobacterial blooms have brought great harm to human and aquatic organisms, attracting a wide public health attention.					
31740324	14	73	theme	Ms	1495:1496	arg1	magnet					1487:1492	a magnet	1485:1492	a magnet (Ms 21.5 emug-1)	1485:1509	YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1).					
31740324	14	73	theme	Ms	1495:1496	arg1	emug-1					1503:1508	Ms 21.5 emug-1	1495:1508	Ms 21.5 emug-1	1495:1508	YF1 exhibited significantly increased MC-LR-degrading capabilities after recycling and reusing which degradation rate reached 1.50 μg/mL/h in the sixth cycle, and it was easily recycled by using a magnet (Ms 21.5 emug-1).					
31740324	15	74	dep	genes	1584:1588	arg1	mlrC					1606:1609	mlrC	1606:1609	mlrC	1606:1609	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	74	dep	genes	1584:1588	arg1	mlrB					1597:1600	mlrB	1597:1600	mlrB	1597:1600	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	74	dep	genes	1584:1588	arg1	mlrA					1591:1594	mlrA	1591:1594	mlrA	1591:1594	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	15	74	dep	genes	1584:1588	arg1	genes					1584:1588	three degradation related genes	1558:1588	three degradation related genes (mlrA, mlrB and mlrC)	1558:1610	Two intermediates (tetrapeptide and Adda) and three degradation related genes (mlrA, mlrB and mlrC) were obtained in this study and the pathway for the degradation was proposed.					
31740324	5	75	theme	CTS/Sphingopyxis	762:777	arg1	sp					779:780	Fe3O4@CTS/Sphingopyxis sp	756:780	Fe3O4@CTS/Sphingopyxis sp	756:780	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	10	76	dep	%	1022:1022	arg1	v/v					1025:1027	v/v	1025:1027	v/v	1025:1027	The results showed that the optimal immobilization conditions containing 1.0 % (v/v) glutaraldehyde, immobilization for 4 h at 30 ℃.					
31740324	3	77	with	deal	294:297	arg1	contamination					310:322	MC-LR contamination	304:322	MC-LR contamination	304:322	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	3	78	theme	sustainable	404:414	arg1	biodegradation					416:429	the high-efficient and sustainable biodegradation	381:429	the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp	381:488	To deal with MC-LR contamination, we synthesized a novel bio-functionalized composite for the high-efficient and sustainable biodegradation of microcystin-LR by covalent immobilizing Sphingopyxis sp.					
31740324	4	79	theme	Fe3O4	543:547	arg1	particles					532:540	chitosan-grafted Fe3O4 magnetic particles	500:540	chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS)	500:552	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	4	79	theme	Fe3O4	543:547	arg1	CTS					549:551	Fe3O4@CTS	543:551	Fe3O4@CTS	543:551	YF1 onto chitosan-grafted Fe3O4 magnetic particles (Fe3O4@CTS).					
31740324	12	80	theme	wide	1175:1178	arg1	torlerance					1180:1189	a wide torlerance	1173:1189	a wide torlerance to pH (6.0-9.0) and temperature (25-35 ℃)	1173:1231	YF1 showed an attractive degradation performance which possesed a wide torlerance to pH (6.0-9.0) and temperature (25-35 ℃).					
31740324	5	81	used	utilized	702:709	arg2	Fourier					559:565	The Fourier	555:565	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM)	555:695	The Fourier transform infrared spectroscopy (FTIR), Field emission scanning electron microscopy (SEM) and vibrating sample magnetometry (VSM) were utilized to characterize the structural properties of Fe3O4@CTS/Sphingopyxis sp.					
31740324	8	82	theme	@	879:879	arg1	sp					897:898	Fe3O4@CTS/Sphingopyxis sp	874:898	Fe3O4@CTS/Sphingopyxis sp	874:898	And the MC-LR-degrading capabilities of Fe3O4@CTS/Sphingopyxis sp.					
31740324	16	83	dep	revealed	1704:1711	arg1	sp					1741:1742	Fe3O4@CTS/Sphingopyxis sp	1718:1742	Fe3O4@CTS/Sphingopyxis sp	1718:1742	These results revealed that Fe3O4@CTS/Sphingopyxis sp.					
29889600	4	0	theme	glucansucrase	677:689	arg1	activity					691:698	glucansucrase activity	677:698	glucansucrase activity	677:698	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	6	1	theme	crude	999:1003	arg1	enzyme					1005:1010	the crude enzyme	995:1010	the crude enzyme	995:1010	In addition, the crude enzyme was purified using ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration.					
29889600	5	2	theme	higher	962:967	arg1	fermentation					938:949	24 hr fermentation	932:949	24 hr fermentation	932:949	Optimum results showed that glucansucrase activity was increased to 3.94 ± 0.43 U/mL in 24 hr fermentation, 2.66-fold higher than before.					
29889600	7	3	theme	glucansucrase	1136:1148	arg1	weight					1126:1131	The molecular weight	1112:1131	The molecular weight of glucansucrase	1112:1148	The molecular weight of glucansucrase was determined as approximately 170 kDa by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).					
29889600	7	3	theme	glucansucrase	1136:1148	arg1	170 kDa					1182:1188	170 kDa	1182:1188	170 kDa	1182:1188	The molecular weight of glucansucrase was determined as approximately 170 kDa by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).					
29889600	6	4	theme	gel	1096:1098	arg1	filtration					1100:1109	gel filtration	1096:1109	gel filtration	1096:1109	In addition, the crude enzyme was purified using ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration.					
29889600	7	5	theme	molecular	1116:1124	arg1	weight					1126:1131	The molecular weight	1112:1131	The molecular weight of glucansucrase	1112:1148	The molecular weight of glucansucrase was determined as approximately 170 kDa by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).					
29889600	7	5	theme	molecular	1116:1124	arg1	170 kDa					1182:1188	170 kDa	1182:1188	170 kDa	1182:1188	The molecular weight of glucansucrase was determined as approximately 170 kDa by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).					
29889600	4	6	theme	composite	737:745	arg1	design					747:752	central composite design	729:752	central composite design	729:752	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	0	7	theme	Chinese	107:113	arg1	Sauerkraut					115:124	Chinese Sauerkraut	107:124	Chinese Sauerkraut	107:124	Optimization and purification of glucansucrase produced by Leuconostoc mesenteroides DRP2-19 isolated from Chinese Sauerkraut.					
29889600	5	8	theme	24 hr	932:936	arg1	fermentation					938:949	24 hr fermentation	932:949	24 hr fermentation	932:949	Optimum results showed that glucansucrase activity was increased to 3.94 ± 0.43 U/mL in 24 hr fermentation, 2.66-fold higher than before.					
29889600	7	9	theme	sulfate-polyacrylamide	1208:1229	arg1	electrophoresis					1235:1249	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	1193:1249	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	1193:1260	The molecular weight of glucansucrase was determined as approximately 170 kDa by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).					
29889600	7	9	theme	sulfate-polyacrylamide	1208:1229	arg1	SDS-PAGE					1252:1259	SDS-PAGE	1252:1259	SDS-PAGE	1252:1259	The molecular weight of glucansucrase was determined as approximately 170 kDa by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).					
29889600	2	10	theme	glucansucrase	286:298	arg1	production					300:309	industrial glucansucrase production	275:309	industrial glucansucrase production of L. mesenteroides DRP2-19	275:337	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	1	11	theme	glucansucrase	180:192	arg1	yield					171:175	high yield	166:175	high yield of glucansucrase	166:192	Strain DRP2-19 was detected to produce high yield of glucansucrase in MRS broth, which was identified to be Leuconostoc mesenteroides.					
29889600	7	12	theme	gel	1231:1233	arg1	electrophoresis					1235:1249	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis	1193:1249	Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)	1193:1260	The molecular weight of glucansucrase was determined as approximately 170 kDa by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).					
29889600	7	12	theme	gel	1231:1233	arg1	SDS-PAGE					1252:1259	SDS-PAGE	1252:1259	SDS-PAGE	1252:1259	The molecular weight of glucansucrase was determined as approximately 170 kDa by Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).					
29889600	4	13	theme	initial	827:833	arg1	pH					835:836	initial pH 5.56	827:841	initial pH 5.56	827:841	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	2	14	theme	industrial	275:284	arg1	production					300:309	industrial glucansucrase production	275:309	industrial glucansucrase production of L. mesenteroides DRP2-19	275:337	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	2	15	theme	best	453:456	arg1	condition					469:477	the best production condition	449:477	the best production condition	449:477	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	4	16	theme	central	729:735	arg1	design					747:752	central composite design	729:752	central composite design	729:752	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	4	17	from	effects	640:646	arg1	activity					691:698	glucansucrase activity	677:698	glucansucrase activity	677:698	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	1	18	theme	MRS	197:199	arg1	broth					201:205	MRS broth	197:205	MRS broth	197:205	Strain DRP2-19 was detected to produce high yield of glucansucrase in MRS broth, which was identified to be Leuconostoc mesenteroides.					
29889600	5	19	theme	Optimum	844:850	arg1	results					852:858	Optimum results	844:858	Optimum results	844:858	Optimum results showed that glucansucrase activity was increased to 3.94 ± 0.43 U/mL in 24 hr fermentation, 2.66-fold higher than before.					
29889600	2	20	theme	DRP2-19	331:337	arg1	production					300:309	industrial glucansucrase production	275:309	industrial glucansucrase production of L. mesenteroides DRP2-19	275:337	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	5	21	dep	higher	962:967	arg1	before					974:979	before	974:979	before	974:979	Optimum results showed that glucansucrase activity was increased to 3.94 ± 0.43 U/mL in 24 hr fermentation, 2.66-fold higher than before.					
29889600	2	22	theme	surface	387:393	arg1	method					395:400	response surface method	378:400	response surface method	378:400	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	3	23	theme	initial	541:547	arg1	pH					549:550	initial pH	541:550	initial pH	541:550	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	3	23	theme	initial	541:547	arg1	experiment					510:519	experiment	510:519	experiment	510:519	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	3	23	theme	initial	541:547	arg1	Ca2+					531:534	Ca2+	531:534	Ca2+	531:534	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	3	23	theme	initial	541:547	arg1	sucrose					522:528	sucrose	522:528	sucrose	522:528	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	3	23	theme	initial	541:547	arg1	factors					590:596	the most significant factors	569:596	the most significant factors for glucansucrase production	569:625	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	2	24	theme	L.	314:315	arg1	DRP2-19					331:337	L. mesenteroides DRP2-19	314:337	L. mesenteroides DRP2-19	314:337	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	4	25	theme	factors	666:672	arg1	effects					640:646	effects	640:646	effects of the three main factors on glucansucrase activity	640:698	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	8	26	theme	final	1311:1315	arg1	activity					1326:1333	a final specific activity	1309:1333	a final specific activity of 338.56 U/mg protein	1309:1356	The enzyme was purified 15.77-fold and showed a final specific activity of 338.56 U/mg protein.					
29889600	6	27	theme	ion-exchange	1063:1074	arg1	chromatography					1076:1089	ion-exchange chromatography	1063:1089	ion-exchange chromatography	1063:1089	In addition, the crude enzyme was purified using ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration.					
29889600	6	28	theme	sulfate	1040:1046	arg1	precipitation					1048:1060	ammonium sulfate precipitation	1031:1060	ammonium sulfate precipitation	1031:1060	In addition, the crude enzyme was purified using ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration.					
29889600	0	29	theme	glucansucrase	33:45	arg1	purification					17:28	purification	17:28	purification	17:28	Optimization and purification of glucansucrase produced by Leuconostoc mesenteroides DRP2-19 isolated from Chinese Sauerkraut.					
29889600	0	29	theme	glucansucrase	33:45	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization and purification of glucansucrase produced by Leuconostoc mesenteroides DRP2-19 isolated from Chinese Sauerkraut.					
29889600	4	30	theme	Ca2+	805:808	arg1	0.21 mmol/L					810:820	Ca2+ 0.21 mmol/L	805:820	Ca2+ 0.21 mmol/L	805:820	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	6	31	theme	ammonium	1031:1038	arg1	precipitation					1048:1060	ammonium sulfate precipitation	1031:1060	ammonium sulfate precipitation	1031:1060	In addition, the crude enzyme was purified using ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration.					
29889600	0	32	theme	Leuconostoc	59:69	arg1	mesenteroides					71:83	Leuconostoc mesenteroides DRP2-19	59:91	Leuconostoc mesenteroides DRP2-19 isolated from Chinese Sauerkraut	59:124	Optimization and purification of glucansucrase produced by Leuconostoc mesenteroides DRP2-19 isolated from Chinese Sauerkraut.					
29889600	5	33	theme	glucansucrase	872:884	arg1	activity					886:893	glucansucrase activity	872:893	glucansucrase activity	872:893	Optimum results showed that glucansucrase activity was increased to 3.94 ± 0.43 U/mL in 24 hr fermentation, 2.66-fold higher than before.					
29889600	8	34	theme	protein	1350:1356	arg1	activity					1326:1333	a final specific activity	1309:1333	a final specific activity of 338.56 U/mg protein	1309:1356	The enzyme was purified 15.77-fold and showed a final specific activity of 338.56 U/mg protein.					
29889600	2	35	theme	production	458:467	arg1	condition					469:477	the best production condition	449:477	the best production condition	449:477	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	0	36	attach	isolated	93:100	arg2	mesenteroides					71:83	Leuconostoc mesenteroides DRP2-19	59:91	Leuconostoc mesenteroides DRP2-19 isolated from Chinese Sauerkraut	59:124	Optimization and purification of glucansucrase produced by Leuconostoc mesenteroides DRP2-19 isolated from Chinese Sauerkraut.					
29889600	0	36	attach	isolated	93:100	arg1	Sauerkraut					115:124	Chinese Sauerkraut	107:124	Chinese Sauerkraut	107:124	Optimization and purification of glucansucrase produced by Leuconostoc mesenteroides DRP2-19 isolated from Chinese Sauerkraut.					
29889600	8	37	theme	specific	1317:1324	arg1	activity					1326:1333	a final specific activity	1309:1333	a final specific activity of 338.56 U/mg protein	1309:1356	The enzyme was purified 15.77-fold and showed a final specific activity of 338.56 U/mg protein.					
29889600	4	38	theme	sucrose	786:792	arg1	35.87 g/L					794:802	sucrose 35.87 g/L	786:802	sucrose 35.87 g/L	786:802	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	4	38	theme	sucrose	786:792	arg1	composition					770:780	the optimum composition	758:780	the optimum composition	758:780	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	2	39	theme	one-factor	342:351	arg1	test					353:356	a one-factor test	340:356	a one-factor test	340:356	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	2	40	theme	mesenteroides	317:329	arg1	DRP2-19					331:337	L. mesenteroides DRP2-19	314:337	L. mesenteroides DRP2-19	314:337	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	4	41	theme	optimum	762:768	arg1	35.87 g/L					794:802	sucrose 35.87 g/L	786:802	sucrose 35.87 g/L	786:802	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	4	41	theme	optimum	762:768	arg1	composition					770:780	the optimum composition	758:780	the optimum composition	758:780	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	3	42	theme	glucansucrase	602:614	arg1	production					616:625	glucansucrase production	602:625	glucansucrase production	602:625	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	4	43	theme	main	661:664	arg1	factors					666:672	the three main factors	651:672	the three main factors	651:672	Afterwards, effects of the three main factors on glucansucrase activity were further investigated by central composite design and the optimum composition was sucrose 35.87 g/L, Ca2+ 0.21 mmol/L, and initial pH 5.56.					
29889600	8	44	theme	338.56 U/mg	1338:1348	arg1	protein					1350:1356	338.56 U/mg protein	1338:1356	338.56 U/mg protein	1338:1356	The enzyme was purified 15.77-fold and showed a final specific activity of 338.56 U/mg protein.					
29889600	3	45	theme	significant	578:588	arg1	pH					549:550	initial pH	541:550	initial pH	541:550	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	3	45	theme	significant	578:588	arg1	factors					590:596	the most significant factors	569:596	the most significant factors for glucansucrase production	569:625	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	3	45	theme	significant	578:588	arg1	sucrose					522:528	sucrose	522:528	sucrose	522:528	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	3	45	theme	significant	578:588	arg1	Ca2+					531:534	Ca2+	531:534	Ca2+	531:534	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	3	45	theme	significant	578:588	arg1	experiment					510:519	experiment	510:519	experiment	510:519	Based on Plackett-Burman (PB) experiment, sucrose, Ca2+, and initial pH were found to be the most significant factors for glucansucrase production.					
29889600	2	46	theme	response	378:385	arg1	method					395:400	response surface method	378:400	response surface method	378:400	In order for industrial glucansucrase production of L. mesenteroides DRP2-19, a one-factor test was conducted, then response surface method was applied to optimize its yield and discover the best production condition.					
29889600	1	47	theme	Leuconostoc	235:245	arg1	mesenteroides					247:259	Leuconostoc mesenteroides	235:259	Leuconostoc mesenteroides	235:259	Strain DRP2-19 was detected to produce high yield of glucansucrase in MRS broth, which was identified to be Leuconostoc mesenteroides.					
29889600	1	48	theme	high	166:169	arg1	yield					171:175	high yield	166:175	high yield of glucansucrase	166:192	Strain DRP2-19 was detected to produce high yield of glucansucrase in MRS broth, which was identified to be Leuconostoc mesenteroides.					
29432560	11	0	dep	DE	1861:1862	arg1	the					1857:1859	the	1857:1859	the	1857:1859	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	10	1	theme	P	1647:1647	arg1	<					1649:1649	P < 0.05	1647:1654	P < 0.05	1647:1654	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	2	2	dep	BW	439:440	arg1	kg					454:455	31.0 ± 1.0 kg	443:455	initial BW: 31.0 ± 1.0 kg	431:455	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	9	3	theme	bulk	1417:1420	arg1	density					1422:1428	greater bulk density	1409:1428	greater bulk density (498.5 g/L)	1409:1440	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	3	theme	bulk	1417:1420	arg1	g/L					1437:1439	498.5 g/L	1431:1439	498.5 g/L	1431:1439	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	5	4	theme	GE	935:936	arg1	ATTD					927:930	the ATTD	923:930	the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog	923:998	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	7	5	theme	average	1080:1086	arg1	%					1117:1117	17.67%	1112:1117	17.67% in wheat midlings	1112:1135	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	5	theme	average	1080:1086	arg1	concentration					1088:1100	The average concentration	1076:1100	The average concentration of CP	1076:1106	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	1	6	theme	different	366:374	arg1	mills					382:386	different flour mills	366:386	different flour mills in the United States	366:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	7	theme	nutrient	157:164	arg1	composition					166:176	nutrient composition	157:176	nutrient composition	157:176	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	4	8	theme	soybean	688:694	arg1	meal					696:699	soybean meal	688:699	soybean meal	688:699	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	1	9	theme	OM	235:236	arg1	composition					166:176	nutrient composition	157:176	nutrient composition	157:176	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	9	theme	OM	235:236	arg1	concentration					255:267	the concentration	251:267	the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States	251:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	9	theme	OM	235:236	arg1	ATTD					218:221	ATTD	218:221	ATTD	218:221	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	9	theme	OM	235:236	arg1	digestibility					203:215	apparent total tract digestibility	182:215	apparent total tract digestibility (ATTD)	182:222	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	7	10	from	%	1300:1300	arg1	midlings					1128:1135	wheat midlings	1122:1135	wheat midlings	1122:1135	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	10	from	%	1300:1300	arg1	dog					1154:1156	red dog	1150:1156	red dog	1150:1156	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	10	from	%	1300:1300	arg1	dog					1277:1279	red dog	1273:1279	red dog	1273:1279	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	10	from	%	1300:1300	arg1	middlings					1311:1319	wheat middlings	1305:1319	wheat middlings	1305:1319	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	3	11	theme	metabolism	598:607	arg1	crates					609:614	metabolism crates	598:614	metabolism crates	598:614	Pigs were individually housed in metabolism crates for total collection of feces and urine.					
29432560	4	12	contain	containing	715:724	arg2	%					754:754	39.4%	750:754	39.4% of one of the 10 sources of wheat middlings or of red dog	750:812	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	12	contain	containing	715:724	arg2	corn					726:729	corn	726:729	corn	726:729	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	12	contain	containing	715:724	arg1	diets					709:713	11 diets	706:713	11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog	706:812	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	12	contain	containing	715:724	arg2	one					759:761	one	759:761	one	759:761	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	12	contain	containing	715:724	arg2	sources					773:779	the 10 sources	766:779	the 10 sources of wheat middlings or of red dog	766:812	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	12	contain	containing	715:724	arg2	meal					740:743	soybean meal	732:743	soybean meal	732:743	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	7	13	from	%	1117:1117	arg1	midlings					1128:1135	wheat midlings	1122:1135	wheat midlings	1122:1135	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	13	from	%	1117:1117	arg1	dog					1154:1156	red dog	1150:1156	red dog	1150:1156	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	13	from	%	1117:1117	arg1	dog					1277:1279	red dog	1273:1279	red dog	1273:1279	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	13	from	%	1117:1117	arg1	middlings					1311:1319	wheat middlings	1305:1319	wheat middlings	1305:1319	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	10	14	theme	wheat	1574:1578	arg1	middlings					1580:1588	wheat middlings	1574:1588	wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively)	1574:1635	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	0	15	theme	growing	101:107	arg1	pigs					109:112	growing pigs	101:112	growing pigs	101:112	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	1	16	theme	middlings	305:313	arg1	sources					288:294	10 sources	285:294	10 sources of wheat middlings	285:313	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	17	theme	total	191:195	arg1	ATTD					218:221	ATTD	218:221	ATTD	218:221	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	17	theme	total	191:195	arg1	digestibility					203:215	apparent total tract digestibility	182:215	apparent total tract digestibility (ATTD)	182:222	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	10	18	dep	less	1641:1644	arg1	<					1649:1649	P < 0.05	1647:1654	P < 0.05	1647:1654	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	19	theme	red	1665:1667	arg1	dog					1669:1671	red dog	1665:1671	red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively)	1665:1719	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	20	theme	GE	1551:1552	arg1	ATTD					1543:1546	The average ATTD	1531:1546	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively)	1531:1635	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	20	theme	GE	1551:1552	arg1	less					1641:1644	less	1641:1644	less	1641:1644	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	9	21	theme	particle	1454:1461	arg1	size					1463:1466	smaller particle size	1446:1466	smaller particle size (146 µm)	1446:1475	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	21	theme	particle	1454:1461	arg1	µm					1473:1474	146 µm	1469:1474	146 µm	1469:1474	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	12	22	contain	contains	1943:1950	arg1	conclusion					1915:1924	conclusion	1915:1924	conclusion	1915:1924	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	12	22	contain	contains	1943:1950	arg2	middlings					1933:1941	wheat middlings	1927:1941	wheat middlings	1927:1941	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	5	23	from	ATTD	927:930	arg1	source					963:968	each source	958:968	each source of wheat middlings or red dog	958:998	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	8	24	theme	Red	1322:1324	arg1	dog					1326:1328	Red dog	1322:1328	Red dog	1322:1328	Red dog also contained more starch (42.98%) than wheat middlings (20.28%).					
29432560	11	25	dep	2,990	1786:1790	arg1	DM					1810:1811	kcal/kg DM	1802:1811	kcal/kg DM	1802:1811	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	7	26	theme	fiber	1238:1242	arg1	%					1268:1268	2.44 and 13.90%	1254:1268	2.44 and 13.90% in red dog	1254:1279	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	26	theme	fiber	1238:1242	arg1	concentration					1167:1179	the concentration	1163:1179	the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF)	1163:1248	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	2	27	theme	d	554:554	arg1	periods					556:562	eight 14 d periods	545:562	eight 14 d periods	545:562	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	7	28	theme	wheat	1305:1309	arg1	middlings					1311:1319	wheat middlings	1305:1319	wheat middlings	1305:1319	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	29	theme	total	1224:1228	arg1	TDF					1245:1247	TDF	1245:1247	TDF	1245:1247	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	29	theme	total	1224:1228	arg1	fiber					1238:1242	total dietary fiber	1224:1242	total dietary fiber (TDF)	1224:1248	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	3	30	theme	urine	650:654	arg1	collection					626:635	total collection	620:635	total collection of feces and urine	620:654	Pigs were individually housed in metabolism crates for total collection of feces and urine.					
29432560	1	31	theme	red	334:336	arg1	dog					338:340	red dog	334:340	red dog that were obtained from different flour mills in the United States	334:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	32	from	composition	166:176	arg1	sources					288:294	10 sources	285:294	10 sources of wheat middlings	285:313	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	32	from	composition	166:176	arg1	source					324:329	1 source	322:329	1 source of red dog that were obtained from different flour mills in the United States	322:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	5	33	theme	wheat	973:977	arg1	middlings					979:987	wheat middlings	973:987	wheat middlings	973:987	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	3	34	theme	feces	640:644	arg1	collection					626:635	total collection	620:635	total collection of feces and urine	620:654	Pigs were individually housed in metabolism crates for total collection of feces and urine.					
29432560	5	35	from	ATTD	835:838	arg1	diets					870:874	all diets	866:874	all diets	866:874	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	5	36	theme	NDF	859:861	arg1	ATTD					835:838	The ATTD	831:838	The ATTD of GE, DM, OM, and NDF in all diets	831:874	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	4	37	theme	sources	773:779	arg1	one					759:761	one	759:761	one	759:761	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	37	theme	sources	773:779	arg1	sources					773:779	the 10 sources	766:779	the 10 sources of wheat middlings or of red dog	766:812	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	9	38	theme	wheat	1491:1495	arg1	middlings					1497:1505	wheat middlings	1491:1505	wheat middlings (315.1 g/L and 783 µm)	1491:1528	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	38	theme	wheat	1491:1495	arg1	g/L					1514:1516	315.1 g/L	1508:1516	315.1 g/L	1508:1516	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	38	theme	wheat	1491:1495	arg1	µm					1526:1527	783 µm	1522:1527	783 µm	1522:1527	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	2	39	with	design	507:512	arg1	periods					556:562	eight 14 d periods	545:562	eight 14 d periods	545:562	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	2	39	with	design	507:512	arg1	treatments					530:539	12 dietary treatments	519:539	12 dietary treatments	519:539	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	1	40	theme	ME	279:280	arg1	composition					166:176	nutrient composition	157:176	nutrient composition	157:176	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	40	theme	ME	279:280	arg1	concentration					255:267	the concentration	251:267	the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States	251:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	40	theme	ME	279:280	arg1	ATTD					218:221	ATTD	218:221	ATTD	218:221	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	40	theme	ME	279:280	arg1	digestibility					203:215	apparent total tract digestibility	182:215	apparent total tract digestibility (ATTD)	182:222	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	7	41	theme	ether	1200:1204	arg1	AEE					1215:1217	AEE	1215:1217	AEE	1215:1217	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	41	theme	ether	1200:1204	arg1	extract					1206:1212	acid hydrolyzed ether extract	1184:1212	acid hydrolyzed ether extract (AEE)	1184:1218	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	12	42	theme	GE	2008:2009	arg1	greater					2028:2034	greater	2028:2034	greater	2028:2034	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	12	42	theme	GE	2008:2009	arg1	ATTD					2000:2003	the ATTD	1996:2003	the ATTD of GE and nutrients	1996:2023	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	0	43	theme	red	86:88	arg1	dog					90:92	red dog	86:92	red dog	86:92	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	7	44	theme	acid	1184:1187	arg1	AEE					1215:1217	AEE	1215:1217	AEE	1215:1217	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	44	theme	acid	1184:1187	arg1	extract					1206:1212	acid hydrolyzed ether extract	1184:1212	acid hydrolyzed ether extract (AEE)	1184:1218	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	2	45	theme	growing	417:423	arg1	pigs					425:428	Twelve growing pigs	410:428	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg)	410:456	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	2	46	theme	Youden	493:498	arg1	design					507:512	a 12 × 8 Youden square design	484:512	a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods	484:562	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	10	47	from	ATTD	1543:1546	arg1	middlings					1580:1588	wheat middlings	1574:1588	wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively)	1574:1635	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	11	48	from	DE	1861:1862	arg1	dog					1878:1880	red dog	1874:1880	red dog (3,408 and 3,292 kcal/kg DM)	1874:1909	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	11	48	from	DE	1861:1862	arg1	DM					1907:1908	3,408 and 3,292 kcal/kg DM	1883:1908	3,408 and 3,292 kcal/kg DM	1883:1908	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	4	49	theme	red	806:808	arg1	dog					810:812	red dog	806:812	red dog	806:812	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	11	50	from	concentrations	1734:1747	arg1	middlings					1771:1779	wheat middlings	1765:1779	wheat middlings	1765:1779	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	0	51	theme	Nutrient	0:7	arg1	composition					9:19	Nutrient composition	0:19	Nutrient composition	0:19	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	5	52	theme	direct	901:906	arg1	procedure					908:916	the direct procedure	897:916	the direct procedure	897:916	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	11	53	from	ME	1868:1869	arg1	dog					1878:1880	red dog	1874:1880	red dog (3,408 and 3,292 kcal/kg DM)	1874:1909	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	11	53	from	ME	1868:1869	arg1	DM					1907:1908	3,408 and 3,292 kcal/kg DM	1883:1908	3,408 and 3,292 kcal/kg DM	1883:1908	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	13	54	theme	DE	2108:2109	arg1	concentrations					2090:2103	concentrations	2090:2103	concentrations of DE and ME	2090:2116	As a consequence, concentrations of DE and ME are greater in red dog than in wheat middlings.					
29432560	4	55	theme	wheat	784:788	arg1	middlings					790:798	wheat middlings	784:798	wheat middlings	784:798	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	56	theme	basal	659:663	arg1	diet					665:668	A basal diet	657:668	A basal diet	657:668	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	5	57	theme	dog	996:998	arg1	source					963:968	each source	958:968	each source of wheat middlings or red dog	958:998	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	2	58	dep	pigs	425:428	arg1	BW					439:440	initial BW	431:440	initial BW: 31.0 ± 1.0 kg	431:455	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	13	59	theme	ME	2115:2116	arg1	concentrations					2090:2103	concentrations	2090:2103	concentrations of DE and ME	2090:2116	As a consequence, concentrations of DE and ME are greater in red dog than in wheat middlings.					
29432560	13	60	theme	wheat	2149:2153	arg1	middlings					2155:2163	wheat middlings	2149:2163	wheat middlings	2149:2163	As a consequence, concentrations of DE and ME are greater in red dog than in wheat middlings.					
29432560	12	61	theme	red	1984:1986	arg1	dog					1988:1990	red dog	1984:1990	red dog	1984:1990	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	1	62	theme	United	395:400	arg1	States					402:407	the United States	391:407	the United States	391:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	63	theme	DE	272:273	arg1	composition					166:176	nutrient composition	157:176	nutrient composition	157:176	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	63	theme	DE	272:273	arg1	concentration					255:267	the concentration	251:267	the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States	251:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	63	theme	DE	272:273	arg1	ATTD					218:221	ATTD	218:221	ATTD	218:221	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	63	theme	DE	272:273	arg1	digestibility					203:215	apparent total tract digestibility	182:215	apparent total tract digestibility (ATTD)	182:222	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	7	64	theme	red	1150:1152	arg1	dog					1154:1156	red dog	1150:1156	red dog	1150:1156	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	10	65	dep	middlings	1580:1588	arg1	%					1595:1595	67.2%	1591:1595	67.2%	1591:1595	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	65	dep	middlings	1580:1588	arg1	%					1602:1602	71.2%	1598:1602	71.2%	1598:1602	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	65	dep	middlings	1580:1588	arg1	%					1609:1609	72.9%	1605:1609	72.9%	1605:1609	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	65	dep	middlings	1580:1588	arg1	%					1620:1620	53.0%	1616:1620	53.0%	1616:1620	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	11	66	theme	red	1874:1876	arg1	dog					1878:1880	red dog	1874:1880	red dog (3,408 and 3,292 kcal/kg DM)	1874:1909	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	11	66	theme	red	1874:1876	arg1	DM					1907:1908	3,408 and 3,292 kcal/kg DM	1883:1908	3,408 and 3,292 kcal/kg DM	1883:1908	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	11	67	theme	P	1842:1842	arg1	<					1844:1844	P < 0.05	1842:1849	P < 0.05	1842:1849	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	10	68	theme	DM	1555:1556	arg1	ATTD					1543:1546	The average ATTD	1531:1546	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively)	1531:1635	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	68	theme	DM	1555:1556	arg1	less					1641:1644	less	1641:1644	less	1641:1644	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	12	69	theme	red	2039:2041	arg1	dog					2043:2045	red dog	2039:2045	red dog	2039:2045	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	1	70	from	concentration	255:267	arg1	sources					288:294	10 sources	285:294	10 sources of wheat middlings	285:313	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	70	from	concentration	255:267	arg1	source					324:329	1 source	322:329	1 source of red dog that were obtained from different flour mills in the United States	322:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	12	71	theme	wheat	2055:2059	arg1	middlings					2061:2069	wheat middlings	2055:2069	wheat middlings	2055:2069	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	1	72	theme	GE	227:228	arg1	composition					166:176	nutrient composition	157:176	nutrient composition	157:176	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	72	theme	GE	227:228	arg1	concentration					255:267	the concentration	251:267	the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States	251:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	72	theme	GE	227:228	arg1	ATTD					218:221	ATTD	218:221	ATTD	218:221	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	72	theme	GE	227:228	arg1	digestibility					203:215	apparent total tract digestibility	182:215	apparent total tract digestibility (ATTD)	182:222	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	7	73	theme	wheat	1122:1126	arg1	midlings					1128:1135	wheat midlings	1122:1135	wheat midlings	1122:1135	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	9	74	theme	greater	1409:1415	arg1	density					1422:1428	greater bulk density	1409:1428	greater bulk density (498.5 g/L)	1409:1440	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	74	theme	greater	1409:1415	arg1	g/L					1437:1439	498.5 g/L	1431:1439	498.5 g/L	1431:1439	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	11	75	theme	average	1726:1732	arg1	concentrations					1734:1747	The average concentrations	1722:1747	The average concentrations of DE and ME in wheat middlings	1722:1779	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	11	75	theme	average	1726:1732	arg1	2,990					1786:1790	2,990	1786:1790	2,990	1786:1790	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	1	76	theme	DM	231:232	arg1	composition					166:176	nutrient composition	157:176	nutrient composition	157:176	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	76	theme	DM	231:232	arg1	concentration					255:267	the concentration	251:267	the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States	251:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	76	theme	DM	231:232	arg1	ATTD					218:221	ATTD	218:221	ATTD	218:221	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	76	theme	DM	231:232	arg1	digestibility					203:215	apparent total tract digestibility	182:215	apparent total tract digestibility (ATTD)	182:222	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	7	77	theme	CP	1105:1106	arg1	%					1117:1117	17.67%	1112:1117	17.67% in wheat midlings	1112:1135	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	77	theme	CP	1105:1106	arg1	concentration					1088:1100	The average concentration	1076:1100	The average concentration of CP	1076:1106	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	11	78	theme	ME	1759:1760	arg1	concentrations					1734:1747	The average concentrations	1722:1747	The average concentrations of DE and ME in wheat middlings	1722:1779	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	11	78	theme	ME	1759:1760	arg1	2,990					1786:1790	2,990	1786:1790	2,990	1786:1790	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	10	79	dep	dog	1669:1671	arg1	%					1679:1679	79.35%	1674:1679	79.35%	1674:1679	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	79	dep	dog	1669:1671	arg1	%					1686:1686	82.9%	1682:1686	82.9%	1682:1686	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	79	dep	dog	1669:1671	arg1	%					1693:1693	86.6%	1689:1693	86.6%	1689:1693	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	79	dep	dog	1669:1671	arg1	%					1704:1704	58.7%	1700:1704	58.7%	1700:1704	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	2	80	theme	×	489:489	arg1	design					507:512	a 12 × 8 Youden square design	484:512	a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods	484:562	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	10	81	theme	OM	1559:1560	arg1	ATTD					1543:1546	The average ATTD	1531:1546	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively)	1531:1635	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	81	theme	OM	1559:1560	arg1	less					1641:1644	less	1641:1644	less	1641:1644	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	12	82	theme	nutrients	2015:2023	arg1	greater					2028:2034	greater	2028:2034	greater	2028:2034	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	12	82	theme	nutrients	2015:2023	arg1	ATTD					2000:2003	the ATTD	1996:2003	the ATTD of GE and nutrients	1996:2023	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	1	83	theme	flour	376:380	arg1	mills					382:386	different flour mills	366:386	different flour mills in the United States	366:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	11	84	theme	DE	1752:1753	arg1	concentrations					1734:1747	The average concentrations	1722:1747	The average concentrations of DE and ME in wheat middlings	1722:1779	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	11	84	theme	DE	1752:1753	arg1	2,990					1786:1790	2,990	1786:1790	2,990	1786:1790	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	12	85	theme	wheat	1927:1931	arg1	middlings					1933:1941	wheat middlings	1927:1941	wheat middlings	1927:1941	In conclusion, wheat middlings contains more fiber and less starch than red dog and the ATTD of GE and nutrients is greater in red dog than in wheat middlings.					
29432560	1	86	theme	apparent	182:189	arg1	ATTD					218:221	ATTD	218:221	ATTD	218:221	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	86	theme	apparent	182:189	arg1	digestibility					203:215	apparent total tract digestibility	182:215	apparent total tract digestibility (ATTD)	182:222	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	4	87	theme	soybean	732:738	arg1	meal					740:743	soybean meal	732:743	soybean meal	732:743	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	11	88	theme	kcal/kg	1802:1808	arg1	DM					1810:1811	kcal/kg DM	1802:1811	kcal/kg DM	1802:1811	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	9	89	theme	smaller	1446:1452	arg1	size					1463:1466	smaller particle size	1446:1466	smaller particle size (146 µm)	1446:1475	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	89	theme	smaller	1446:1452	arg1	µm					1473:1474	146 µm	1469:1474	146 µm	1469:1474	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	5	90	theme	GE	843:844	arg1	ATTD					835:838	The ATTD	831:838	The ATTD of GE, DM, OM, and NDF in all diets	831:874	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	1	91	theme	tract	197:201	arg1	ATTD					218:221	ATTD	218:221	ATTD	218:221	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	91	theme	tract	197:201	arg1	digestibility					203:215	apparent total tract digestibility	182:215	apparent total tract digestibility (ATTD)	182:222	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	7	92	theme	dietary	1230:1236	arg1	TDF					1245:1247	TDF	1245:1247	TDF	1245:1247	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	92	theme	dietary	1230:1236	arg1	fiber					1238:1242	total dietary fiber	1224:1242	total dietary fiber (TDF)	1224:1248	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	2	93	theme	initial	431:437	arg1	BW					439:440	initial BW	431:440	initial BW: 31.0 ± 1.0 kg	431:455	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	5	94	theme	DM	847:848	arg1	ATTD					835:838	The ATTD	831:838	The ATTD of GE, DM, OM, and NDF in all diets	831:874	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	1	95	from	digestibility	203:215	arg1	sources					288:294	10 sources	285:294	10 sources of wheat middlings	285:313	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	95	from	digestibility	203:215	arg1	source					324:329	1 source	322:329	1 source of red dog that were obtained from different flour mills in the United States	322:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	7	96	from	%	1268:1268	arg1	midlings					1128:1135	wheat midlings	1122:1135	wheat midlings	1122:1135	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	96	from	%	1268:1268	arg1	dog					1154:1156	red dog	1150:1156	red dog	1150:1156	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	96	from	%	1268:1268	arg1	dog					1277:1279	red dog	1273:1279	red dog	1273:1279	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	96	from	%	1268:1268	arg1	middlings					1311:1319	wheat middlings	1305:1319	wheat middlings	1305:1319	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	11	97	theme	kcal/kg	1899:1905	arg1	dog					1878:1880	red dog	1874:1880	red dog (3,408 and 3,292 kcal/kg DM)	1874:1909	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	11	97	theme	kcal/kg	1899:1905	arg1	DM					1907:1908	3,408 and 3,292 kcal/kg DM	1883:1908	3,408 and 3,292 kcal/kg DM	1883:1908	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	3	98	theme	total	620:624	arg1	collection					626:635	total collection	620:635	total collection of feces and urine	620:654	Pigs were individually housed in metabolism crates for total collection of feces and urine.					
29432560	2	99	theme	±	448:448	arg1	kg					454:455	31.0 ± 1.0 kg	443:455	initial BW: 31.0 ± 1.0 kg	431:455	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	0	100	theme	wheat	66:70	arg1	middlings					72:80	wheat middlings	66:80	wheat middlings	66:80	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	1	101	theme	dog	338:340	arg1	source					324:329	1 source	322:329	1 source of red dog that were obtained from different flour mills in the United States	322:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	10	102	theme	average	1535:1541	arg1	ATTD					1543:1546	The average ATTD	1531:1546	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively)	1531:1635	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	102	theme	average	1535:1541	arg1	less					1641:1644	less	1641:1644	less	1641:1644	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	7	103	theme	extract	1206:1212	arg1	%					1268:1268	2.44 and 13.90%	1254:1268	2.44 and 13.90% in red dog	1254:1279	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	103	theme	extract	1206:1212	arg1	concentration					1167:1179	the concentration	1163:1179	the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF)	1163:1248	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	0	104	from	digestibility	25:37	arg1	middlings					72:80	wheat middlings	66:80	wheat middlings	66:80	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	0	104	from	digestibility	25:37	arg1	dog					90:92	red dog	86:92	red dog	86:92	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	7	105	theme	hydrolyzed	1189:1198	arg1	AEE					1215:1217	AEE	1215:1217	AEE	1215:1217	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	105	theme	hydrolyzed	1189:1198	arg1	extract					1206:1212	acid hydrolyzed ether extract	1184:1212	acid hydrolyzed ether extract (AEE)	1184:1218	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	0	106	from	composition	9:19	arg1	middlings					72:80	wheat middlings	66:80	wheat middlings	66:80	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	0	106	from	composition	9:19	arg1	dog					90:92	red dog	86:92	red dog	86:92	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	5	107	theme	NDF	951:953	arg1	ATTD					927:930	the ATTD	923:930	the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog	923:998	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	13	108	theme	red	2133:2135	arg1	dog					2137:2139	red dog	2133:2139	red dog	2133:2139	As a consequence, concentrations of DE and ME are greater in red dog than in wheat middlings.					
29432560	2	109	theme	square	500:505	arg1	design					507:512	a 12 × 8 Youden square design	484:512	a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods	484:562	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	5	110	theme	OM	851:852	arg1	ATTD					835:838	The ATTD	831:838	The ATTD of GE, DM, OM, and NDF in all diets	831:874	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	11	111	theme	wheat	1765:1769	arg1	middlings					1771:1779	wheat middlings	1765:1779	wheat middlings	1765:1779	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	4	112	theme	one	759:761	arg1	corn					726:729	corn	726:729	corn	726:729	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	112	theme	one	759:761	arg1	meal					740:743	soybean meal	732:743	soybean meal	732:743	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	112	theme	one	759:761	arg1	one					759:761	one	759:761	one	759:761	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	112	theme	one	759:761	arg1	%					754:754	39.4%	750:754	39.4% of one of the 10 sources of wheat middlings or of red dog	750:812	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	4	112	theme	one	759:761	arg1	sources					773:779	the 10 sources	766:779	the 10 sources of wheat middlings or of red dog	766:812	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	1	113	theme	wheat	299:303	arg1	middlings					305:313	wheat middlings	299:313	wheat middlings	299:313	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	8	114	theme	wheat	1371:1375	arg1	middlings					1377:1385	wheat middlings	1371:1385	wheat middlings	1371:1385	Red dog also contained more starch (42.98%) than wheat middlings (20.28%).					
29432560	7	115	theme	red	1273:1275	arg1	dog					1277:1279	red dog	1273:1279	red dog	1273:1279	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	2	116	theme	dietary	522:528	arg1	treatments					530:539	12 dietary treatments	519:539	12 dietary treatments	519:539	Twelve growing pigs (initial BW: 31.0 ± 1.0 kg) were randomly allotted to a 12 × 8 Youden square design with 12 dietary treatments and eight 14 d periods.					
29432560	5	117	theme	middlings	979:987	arg1	source					963:968	each source	958:968	each source of wheat middlings or red dog	958:998	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	4	118	theme	dog	810:812	arg1	sources					773:779	the 10 sources	766:779	the 10 sources of wheat middlings or of red dog	766:812	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	10	119	theme	NDF	1567:1569	arg1	ATTD					1543:1546	The average ATTD	1531:1546	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively)	1531:1635	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	10	119	theme	NDF	1567:1569	arg1	less					1641:1644	less	1641:1644	less	1641:1644	The average ATTD of GE, DM, OM, and NDF in wheat middlings (67.2%, 71.2%, 72.9%, and 53.0%, respectively) was less (P < 0.05) than in red dog (79.35%, 82.9%, 86.6%, and 58.7%, respectively).					
29432560	5	120	theme	red	992:994	arg1	dog					996:998	red dog	992:998	red dog	992:998	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	0	121	theme	energy	42:47	arg1	digestibility					25:37	digestibility	25:37	digestibility	25:37	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	0	121	theme	energy	42:47	arg1	composition					9:19	Nutrient composition	0:19	Nutrient composition	0:19	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	1	122	theme	NDF	243:245	arg1	composition					166:176	nutrient composition	157:176	nutrient composition	157:176	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	122	theme	NDF	243:245	arg1	concentration					255:267	the concentration	251:267	the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States	251:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	122	theme	NDF	243:245	arg1	ATTD					218:221	ATTD	218:221	ATTD	218:221	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	1	122	theme	NDF	243:245	arg1	digestibility					203:215	apparent total tract digestibility	182:215	apparent total tract digestibility (ATTD)	182:222	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	11	123	dep	less	1836:1839	arg1	<					1844:1844	P < 0.05	1842:1849	P < 0.05	1842:1849	The average concentrations of DE and ME in wheat middlings were 2,990 and 2,893 kcal/kg DM, and these values were less (P < 0.05) than the DE and ME in red dog (3,408 and 3,292 kcal/kg DM).					
29432560	9	124	theme	Red	1397:1399	arg1	dog					1401:1403	Red dog	1397:1403	Red dog	1397:1403	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	0	125	theme	nutrients	53:61	arg1	digestibility					25:37	digestibility	25:37	digestibility	25:37	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	0	125	theme	nutrients	53:61	arg1	composition					9:19	Nutrient composition	0:19	Nutrient composition	0:19	Nutrient composition and digestibility of energy and nutrients in wheat middlings and red dog fed to growing pigs.					
29432560	1	126	from	mills	382:386	arg1	States					402:407	the United States	391:407	the United States	391:407	This experiment was designed to determine nutrient composition and apparent total tract digestibility (ATTD) of GE, DM, OM, and NDF and the concentration of DE and ME in 10 sources of wheat middlings and in 1 source of red dog that were obtained from different flour mills in the United States.					
29432560	4	127	theme	middlings	790:798	arg1	sources					773:779	the 10 sources	766:779	the 10 sources of wheat middlings or of red dog	766:812	A basal diet based on corn and soybean meal, and 11 diets containing corn, soybean meal, and 39.4% of one of the 10 sources of wheat middlings or of red dog were formulated.					
29432560	8	128	dep	starch	1350:1355	arg1	%					1363:1363	42.98%	1358:1363	42.98%	1358:1363	Red dog also contained more starch (42.98%) than wheat middlings (20.28%).					
29432560	9	129	contain	had	1405:1407	arg2	density					1422:1428	greater bulk density	1409:1428	greater bulk density (498.5 g/L)	1409:1440	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	129	contain	had	1405:1407	arg2	g/L					1437:1439	498.5 g/L	1431:1439	498.5 g/L	1431:1439	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	129	contain	had	1405:1407	arg2	µm					1473:1474	146 µm	1469:1474	146 µm	1469:1474	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	129	contain	had	1405:1407	arg1	dog					1401:1403	Red dog	1397:1403	Red dog	1397:1403	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	129	contain	had	1405:1407	arg2	size					1463:1466	smaller particle size	1446:1466	smaller particle size (146 µm)	1446:1475	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	130	dep	middlings	1497:1505	arg1	middlings					1497:1505	wheat middlings	1491:1505	wheat middlings (315.1 g/L and 783 µm)	1491:1528	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	130	dep	middlings	1497:1505	arg1	g/L					1514:1516	315.1 g/L	1508:1516	315.1 g/L	1508:1516	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	9	130	dep	middlings	1497:1505	arg1	µm					1526:1527	783 µm	1522:1527	783 µm	1522:1527	Red dog had greater bulk density (498.5 g/L) and smaller particle size (146 µm) compared with wheat middlings (315.1 g/L and 783 µm).					
29432560	5	131	theme	DM	939:940	arg1	ATTD					927:930	the ATTD	923:930	the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog	923:998	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
29432560	7	132	from	%	1145:1145	arg1	midlings					1128:1135	wheat midlings	1122:1135	wheat midlings	1122:1135	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	132	from	%	1145:1145	arg1	dog					1154:1156	red dog	1150:1156	red dog	1150:1156	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	132	from	%	1145:1145	arg1	dog					1277:1279	red dog	1273:1279	red dog	1273:1279	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	7	132	from	%	1145:1145	arg1	middlings					1311:1319	wheat middlings	1305:1319	wheat middlings	1305:1319	The average concentration of CP was 17.67% in wheat midlings and 17.0% in red dog, and the concentration of acid hydrolyzed ether extract (AEE) and total dietary fiber (TDF) was 2.44 and 13.90% in red dog, but 4.07 and 36.45% in wheat middlings.					
29432560	8	133	dep	contained	1335:1343	arg1	%					1393:1393	20.28%	1388:1393	20.28%	1388:1393	Red dog also contained more starch (42.98%) than wheat middlings (20.28%).					
29432560	5	134	theme	OM	943:944	arg1	ATTD					927:930	the ATTD	923:930	the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog	923:998	The ATTD of GE, DM, OM, and NDF in all diets was calculated using the direct procedure, and the ATTD of GE, DM, OM, and NDF in each source of wheat middlings or red dog was calculated by difference.					
31020407	16	0	theme	nectar	2302:2307	arg1	characteristics					2254:2268	the characteristics	2250:2268	the characteristics of P. bifolia and P. chlorantha nectar	2250:2307	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	6	1	theme	habitat	1047:1053	arg1	properties					1055:1064	habitat properties	1047:1064	habitat properties	1047:1064	We found the same primary sugars and AA components in the nectar of both species, although their content varied between the populations according to habitat properties.					
31020407	7	2	with	hexoses	1180:1186	arg1	sucrose					1194:1200	sucrose	1194:1200	sucrose	1194:1200	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	12	3	theme	effectiveness	1885:1897	arg1	context					1874:1880	the context	1870:1880	the context of effectiveness of reproduction	1870:1913	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	12	4	theme	reproduction	1902:1913	arg1	effectiveness					1885:1897	effectiveness	1885:1897	effectiveness of reproduction	1885:1913	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	2	5	theme	pollinator-mediated	353:371	arg1	selection					373:381	pollinator-mediated selection	353:381	pollinator-mediated selection	353:381	Nectar quantity and quality are floral traits that are subjected to pollinator-mediated selection.					
31020407	7	6	contain	had	1115:1117	arg2	ratios					1210:1215	hexoses ratios	1202:1215	hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05)	1202:1385	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	7	6	contain	had	1115:1117	arg1	nectar					1071:1076	The nectar	1067:1076	The nectar of P. bifolia and P. chlorantha	1067:1108	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	7	6	contain	had	1115:1117	arg2	%					1155:1155	9.04-20.68%	1145:1155	9.04-20.68%	1145:1155	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	7	6	contain	had	1115:1117	arg2	concentrations					1129:1142	low sugar concentrations	1119:1142	low sugar concentrations (9.04-20.68%)	1119:1156	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	0	7	theme	Nectar	0:5	arg1	composition					7:17	Nectar composition	0:17	Nectar composition in moth-pollinated Platanthera bifolia and P. chlorantha	0:74	Nectar composition in moth-pollinated Platanthera bifolia and P. chlorantha and its importance for reproductive success.					
31020407	2	8	theme	Nectar	285:290	arg1	quality					305:311	quality	305:311	quality	305:311	Nectar quantity and quality are floral traits that are subjected to pollinator-mediated selection.					
31020407	2	8	theme	Nectar	285:290	arg1	traits					324:329	floral traits	317:329	floral traits that are subjected to pollinator-mediated selection	317:381	Nectar quantity and quality are floral traits that are subjected to pollinator-mediated selection.					
31020407	2	8	theme	Nectar	285:290	arg1	quantity					292:299	quantity	292:299	quantity	292:299	Nectar quantity and quality are floral traits that are subjected to pollinator-mediated selection.					
31020407	8	9	from	selection	1469:1477	arg1	content					1491:1497	fructose content	1482:1497	fructose content	1482:1497	Total sugar content did not influence reproductive success and we found positive selection on fructose content.					
31020407	12	10	theme	chlorantha	1833:1842	arg1	nectar					1805:1810	the nectar	1801:1810	the nectar of P. bifolia and P. chlorantha	1801:1842	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	6	11	theme	primary	916:922	arg1	sugars					924:929	the same primary sugars	907:929	the same primary sugars	907:929	We found the same primary sugars and AA components in the nectar of both species, although their content varied between the populations according to habitat properties.					
31020407	7	12	theme	bifolia	1296:1302	arg1	populations					1304:1314	the P. bifolia populations	1289:1314	the P. bifolia populations was 0.17	1289:1323	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	1	13	theme	amino	204:208	arg1	acids					210:214	proteogenic and non-proteogenic amino acids	172:214	proteogenic and non-proteogenic amino acids	172:214	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	12	14	theme	natural	1847:1853	arg1	populations					1855:1865	natural populations	1847:1865	natural populations in the context of effectiveness of reproduction	1847:1913	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	16	15	theme	nectar	2475:2480	arg1	quality					2482:2488	nectar quality	2475:2488	nectar quality	2475:2488	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	1	16	from	present	221:227	arg1	nectar					236:241	the nectar	232:241	the nectar of Platanthera bifolia and P. chlorantha	232:282	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	5	17	theme	liquid	875:880	arg1	chromatography					882:895	high-performance liquid chromatography	858:895	high-performance liquid chromatography	858:895	Nectar from about 30 flowers (from each population) was sampled and analysed using high-performance liquid chromatography.					
31020407	3	18	from	differences	516:526	arg1	importance					546:555	the importance	542:555	the importance of these nectar characteristics for reproductive success	542:612	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	18	from	differences	516:526	arg1	nectar					531:536	nectar	531:536	nectar	531:536	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	10	19	located	present	1610:1616	arg2	acid					1600:1603	γ-aminobutyric acid	1585:1603	γ-aminobutyric acid	1585:1603	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	10	19	located	present	1610:1616	arg1	population					1630:1639	only one population	1621:1639	only one population of P. chlorantha	1621:1656	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	10	19	located	present	1610:1616	arg2	Cysteine					1572:1579	Cysteine	1572:1579	Cysteine	1572:1579	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	6	20	theme	species	971:977	arg1	nectar					956:961	the nectar	952:961	the nectar of both species	952:977	We found the same primary sugars and AA components in the nectar of both species, although their content varied between the populations according to habitat properties.					
31020407	8	21	theme	Total	1388:1392	arg1	content					1400:1406	Total sugar content	1388:1406	Total sugar content	1388:1406	Total sugar content did not influence reproductive success and we found positive selection on fructose content.					
31020407	3	22	theme	nectar	566:571	arg1	characteristics					573:587	these nectar characteristics	560:587	these nectar characteristics	560:587	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	11	23	theme	non-proteogenic	1689:1703	arg1	AAs					1705:1707	the non-proteogenic AAs	1685:1707	the non-proteogenic AAs	1685:1707	Sarcosine dominated among the non-proteogenic AAs.					
31020407	16	24	theme	pollinators	2426:2436	arg1	requirements					2410:2421	the requirements	2406:2421	the requirements of pollinators	2406:2436	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	12	25	theme	bifolia	1818:1824	arg1	nectar					1805:1810	the nectar	1801:1810	the nectar of P. bifolia and P. chlorantha	1801:1842	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	12	26	from	profiles	1789:1796	arg1	nectar					1805:1810	the nectar	1801:1810	the nectar of P. bifolia and P. chlorantha	1801:1842	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	8	27	theme	reproductive	1426:1437	arg1	success					1439:1445	reproductive success	1426:1445	reproductive success	1426:1445	Total sugar content did not influence reproductive success and we found positive selection on fructose content.					
31020407	14	28	theme	investigated	2007:2018	arg1	species					2020:2026	the investigated species	2003:2026	the investigated species	2003:2026	Pollinators of the investigated species were found to be sensitive to the AAs' taste, from taste classes I and IV.					
31020407	3	29	theme	sister	436:441	arg1	species					443:449	two sister species	432:449	two sister species	432:449	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	29	theme	sister	436:441	arg1	bifolia					455:461	P. bifolia	452:461	P. bifolia	452:461	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	29	theme	sister	436:441	arg1	chlorantha					470:479	P. chlorantha	467:479	P. chlorantha	467:479	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	1	30	dep	Sugars	121:126	arg1	glucose					129:135	glucose	129:135	glucose	129:135	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	30	dep	Sugars	121:126	arg1	Sugars					121:126	Sugars	121:126	Sugars (glucose, fructose and sucrose)	121:158	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	30	dep	Sugars	121:126	arg1	sucrose					151:157	sucrose	151:157	sucrose	151:157	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	30	dep	Sugars	121:126	arg1	fructose					138:145	fructose	138:145	fructose	138:145	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	13	31	theme	reproductive	1953:1964	arg1	r = - 0.79					1975:1984	r = - 0.79	1975:1984	r = - 0.79	1975:1984	Total AAs negatively influenced male reproductive success (r = - 0.79).					
31020407	13	31	theme	reproductive	1953:1964	arg1	success					1966:1972	male reproductive success	1948:1972	male reproductive success (r = - 0.79)	1948:1985	Total AAs negatively influenced male reproductive success (r = - 0.79).					
31020407	3	32	theme	acid	407:410	arg1	composition					417:427	Nectar sugar and amino acid (AA) composition	384:427	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha,	384:480	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	7	33	theme	sugar	1123:1127	arg1	concentrations					1129:1142	low sugar concentrations	1119:1142	low sugar concentrations (9.04-20.68%)	1119:1156	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	7	33	theme	sugar	1123:1127	arg1	%					1155:1155	9.04-20.68%	1145:1155	9.04-20.68%	1145:1155	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	7	34	theme	chlorantha	1099:1108	arg1	nectar					1071:1076	The nectar	1067:1076	The nectar of P. bifolia and P. chlorantha	1067:1108	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	1	35	theme	chlorantha	273:282	arg1	nectar					236:241	the nectar	232:241	the nectar of Platanthera bifolia and P. chlorantha	232:282	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	3	36	theme	Nectar	384:389	arg1	composition					417:427	Nectar sugar and amino acid (AA) composition	384:427	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha,	384:480	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	8	37	theme	fructose	1482:1489	arg1	content					1491:1497	fructose content	1482:1497	fructose content	1482:1497	Total sugar content did not influence reproductive success and we found positive selection on fructose content.					
31020407	13	38	theme	Total	1916:1920	arg1	AAs					1922:1924	Total AAs	1916:1924	Total AAs	1916:1924	Total AAs negatively influenced male reproductive success (r = - 0.79).					
31020407	2	39	theme	floral	317:322	arg1	quality					305:311	quality	305:311	quality	305:311	Nectar quantity and quality are floral traits that are subjected to pollinator-mediated selection.					
31020407	2	39	theme	floral	317:322	arg1	traits					324:329	floral traits	317:329	floral traits that are subjected to pollinator-mediated selection	317:381	Nectar quantity and quality are floral traits that are subjected to pollinator-mediated selection.					
31020407	2	39	theme	floral	317:322	arg1	quantity					292:299	quantity	292:299	quantity	292:299	Nectar quantity and quality are floral traits that are subjected to pollinator-mediated selection.					
31020407	12	40	theme	first	1740:1744	arg1	this					1728:1731	this	1728:1731	this	1728:1731	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	12	40	theme	first	1740:1744	arg1	report					1746:1751	the first report	1736:1751	the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction	1736:1913	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	7	41	dep	populations	1260:1270	arg1	average					1277:1283	the average	1273:1283	the average for the P. bifolia populations was 0.17	1273:1323	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	7	41	dep	populations	1260:1270	arg1	- 0.05					1379:1384	- 0.05	1379:1384	- 0.05	1379:1384	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	7	41	dep	populations	1260:1270	arg1	average					1333:1339	the average	1329:1339	the average for the P. chlorantha populations	1329:1373	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	10	42	theme	chlorantha	1647:1656	arg1	population					1630:1639	only one population	1621:1639	only one population of P. chlorantha	1621:1656	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	16	43	theme	adaptations	2391:2401	arg1	context					2374:2380	the context	2370:2380	the context of their adaptations to the requirements of pollinators	2370:2436	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	4	44	theme	Poland	767:772	arg1	regions					753:759	three regions	747:759	three regions of NE Poland	747:772	Nectar was collected from four P. bifolia and three P. chlorantha populations that exist in different habitats in three regions of NE Poland.					
31020407	16	45	theme	bifolia	2276:2282	arg1	nectar					2302:2307	P. bifolia and P. chlorantha nectar	2273:2307	P. bifolia and P. chlorantha nectar	2273:2307	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	10	46	theme	P.	1644:1645	arg1	chlorantha					1647:1656	P. chlorantha	1644:1656	P. chlorantha	1644:1656	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	16	47	theme	species	2530:2536	arg1	success					2507:2513	reproductive success	2494:2513	reproductive success of the studied species	2494:2536	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	9	48	theme	Platanthera	1551:1561	arg1	species					1563:1569	both Platanthera species	1546:1569	both Platanthera species	1546:1569	In general, 23 different AAs were detected in both Platanthera species.					
31020407	10	49	from	present	1610:1616	arg1	population					1630:1639	only one population	1621:1639	only one population of P. chlorantha	1621:1656	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	15	50	theme	AAs	2168:2170	arg1	success					2141:2147	male reproductive success	2123:2147	male reproductive success	2123:2147	Correlation between male reproductive success and the content of AAs from these groups was 0.79 in both cases.					
31020407	15	50	theme	AAs	2168:2170	arg1	content					2157:2163	the content	2153:2163	the content of AAs from these groups	2153:2188	Correlation between male reproductive success and the content of AAs from these groups was 0.79 in both cases.					
31020407	7	51	theme	P.	1349:1350	arg1	populations					1363:1373	the P. chlorantha populations	1345:1373	the P. chlorantha populations	1345:1373	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	14	52	theme	taste	2079:2083	arg1	classes					2085:2091	taste classes I and IV	2079:2100	taste classes I and IV	2079:2100	Pollinators of the investigated species were found to be sensitive to the AAs' taste, from taste classes I and IV.					
31020407	14	52	theme	taste	2079:2083	arg1	IV					2099:2100	IV	2099:2100	IV	2099:2100	Pollinators of the investigated species were found to be sensitive to the AAs' taste, from taste classes I and IV.					
31020407	14	52	theme	taste	2079:2083	arg1	I					2093:2093	I	2093:2093	I	2093:2093	Pollinators of the investigated species were found to be sensitive to the AAs' taste, from taste classes I and IV.					
31020407	3	53	theme	sugar	391:395	arg1	AA					413:414	AA	413:414	AA	413:414	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	53	theme	sugar	391:395	arg1	acid					407:410	sugar and amino acid	391:410	acid	407:410	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	7	54	theme	chlorantha	1352:1361	arg1	populations					1363:1373	the P. chlorantha populations	1345:1373	the P. chlorantha populations	1345:1373	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	10	55	from	population	1630:1639	arg1	present					1610:1616	present	1610:1616	present	1610:1616	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	6	56	theme	AA	935:936	arg1	components					938:947	AA components	935:947	AA components	935:947	We found the same primary sugars and AA components in the nectar of both species, although their content varied between the populations according to habitat properties.					
31020407	1	57	from	nectar	236:241	arg1	present					221:227	present	221:227	present	221:227	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	9	58	theme	different	1515:1523	arg1	AAs					1525:1527	23 different AAs	1512:1527	23 different AAs	1512:1527	In general, 23 different AAs were detected in both Platanthera species.					
31020407	3	59	theme	interspecies	503:514	arg1	differences					516:526	the interspecies differences	499:526	the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success	499:612	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	1	60	theme	proteogenic	172:182	arg1	acids					210:214	proteogenic and non-proteogenic amino acids	172:214	proteogenic and non-proteogenic amino acids	172:214	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	0	61	from	importance	84:93	arg1	bifolia					50:56	Platanthera bifolia	38:56	Platanthera bifolia	38:56	Nectar composition in moth-pollinated Platanthera bifolia and P. chlorantha and its importance for reproductive success.					
31020407	0	61	from	importance	84:93	arg1	chlorantha					65:74	P. chlorantha	62:74	P. chlorantha	62:74	Nectar composition in moth-pollinated Platanthera bifolia and P. chlorantha and its importance for reproductive success.					
31020407	1	62	theme	non-proteogenic	188:202	arg1	acids					210:214	proteogenic and non-proteogenic amino acids	172:214	proteogenic and non-proteogenic amino acids	172:214	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	6	63	theme	same	911:914	arg1	sugars					924:929	the same primary sugars	907:929	the same primary sugars	907:929	We found the same primary sugars and AA components in the nectar of both species, although their content varied between the populations according to habitat properties.					
31020407	15	64	from	groups	2183:2188	arg1	success					2141:2147	male reproductive success	2123:2147	male reproductive success	2123:2147	Correlation between male reproductive success and the content of AAs from these groups was 0.79 in both cases.					
31020407	15	64	from	groups	2183:2188	arg1	content					2157:2163	the content	2153:2163	the content of AAs from these groups	2153:2188	Correlation between male reproductive success and the content of AAs from these groups was 0.79 in both cases.					
31020407	1	65	attach	present	221:227	arg2	Sugars					121:126	Sugars	121:126	Sugars (glucose, fructose and sucrose)	121:158	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	65	attach	present	221:227	arg2	glucose					129:135	glucose	129:135	glucose	129:135	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	65	attach	present	221:227	arg2	fructose					138:145	fructose	138:145	fructose	138:145	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	65	attach	present	221:227	arg2	acids					210:214	proteogenic and non-proteogenic amino acids	172:214	proteogenic and non-proteogenic amino acids	172:214	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	65	attach	present	221:227	arg2	sucrose					151:157	sucrose	151:157	sucrose	151:157	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	65	attach	present	221:227	arg1	nectar					236:241	the nectar	232:241	the nectar of Platanthera bifolia and P. chlorantha	232:282	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	5	66	theme	high-performance	858:873	arg1	chromatography					882:895	high-performance liquid chromatography	858:895	high-performance liquid chromatography	858:895	Nectar from about 30 flowers (from each population) was sampled and analysed using high-performance liquid chromatography.					
31020407	16	67	theme	reproductive	2494:2505	arg1	success					2507:2513	reproductive success	2494:2513	reproductive success of the studied species	2494:2536	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	15	68	from	0.79	2194:2197	arg1	cases					2207:2211	both cases	2202:2211	both cases	2202:2211	Correlation between male reproductive success and the content of AAs from these groups was 0.79 in both cases.					
31020407	7	69	theme	P.	1293:1294	arg1	populations					1304:1314	the P. bifolia populations	1289:1314	the P. bifolia populations was 0.17	1289:1323	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	3	70	theme	characteristics	573:587	arg1	importance					546:555	the importance	542:555	the importance of these nectar characteristics for reproductive success	542:612	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	70	theme	characteristics	573:587	arg1	nectar					531:536	nectar	531:536	nectar	531:536	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	5	71	from	flowers	796:802	arg1	Nectar					775:780	Nectar	775:780	Nectar from about 30 flowers (from each population)	775:825	Nectar from about 30 flowers (from each population) was sampled and analysed using high-performance liquid chromatography.					
31020407	8	72	theme	sugar	1394:1398	arg1	content					1400:1406	Total sugar content	1388:1406	Total sugar content	1388:1406	Total sugar content did not influence reproductive success and we found positive selection on fructose content.					
31020407	5	73	from	population	815:824	arg1	Nectar					775:780	Nectar	775:780	Nectar from about 30 flowers (from each population)	775:825	Nectar from about 30 flowers (from each population) was sampled and analysed using high-performance liquid chromatography.					
31020407	14	74	dep	classes	2085:2091	arg1	classes					2085:2091	taste classes I and IV	2079:2100	taste classes I and IV	2079:2100	Pollinators of the investigated species were found to be sensitive to the AAs' taste, from taste classes I and IV.					
31020407	14	74	dep	classes	2085:2091	arg1	IV					2099:2100	IV	2099:2100	IV	2099:2100	Pollinators of the investigated species were found to be sensitive to the AAs' taste, from taste classes I and IV.					
31020407	14	74	dep	classes	2085:2091	arg1	I					2093:2093	I	2093:2093	I	2093:2093	Pollinators of the investigated species were found to be sensitive to the AAs' taste, from taste classes I and IV.					
31020407	0	75	from	composition	7:17	arg1	bifolia					50:56	Platanthera bifolia	38:56	Platanthera bifolia	38:56	Nectar composition in moth-pollinated Platanthera bifolia and P. chlorantha and its importance for reproductive success.					
31020407	0	75	from	composition	7:17	arg1	chlorantha					65:74	P. chlorantha	62:74	P. chlorantha	62:74	Nectar composition in moth-pollinated Platanthera bifolia and P. chlorantha and its importance for reproductive success.					
31020407	7	76	dep	had	1115:1117	arg1	both					1110:1113	both	1110:1113	both	1110:1113	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	0	77	theme	reproductive	99:110	arg1	success					112:118	reproductive success	99:118	reproductive success	99:118	Nectar composition in moth-pollinated Platanthera bifolia and P. chlorantha and its importance for reproductive success.					
31020407	4	78	dep	bifolia	667:673	arg1	populations					699:709	populations	699:709	populations that exist in different habitats in three regions of NE Poland	699:772	Nectar was collected from four P. bifolia and three P. chlorantha populations that exist in different habitats in three regions of NE Poland.					
31020407	14	79	theme	species	2020:2026	arg1	Pollinators					1988:1998	Pollinators	1988:1998	Pollinators of the investigated species	1988:2026	Pollinators of the investigated species were found to be sensitive to the AAs' taste, from taste classes I and IV.					
31020407	4	80	theme	different	725:733	arg1	habitats					735:742	different habitats	725:742	different habitats	725:742	Nectar was collected from four P. bifolia and three P. chlorantha populations that exist in different habitats in three regions of NE Poland.					
31020407	13	81	theme	male	1948:1951	arg1	r = - 0.79					1975:1984	r = - 0.79	1975:1984	r = - 0.79	1975:1984	Total AAs negatively influenced male reproductive success (r = - 0.79).					
31020407	13	81	theme	male	1948:1951	arg1	success					1966:1972	male reproductive success	1948:1972	male reproductive success (r = - 0.79)	1948:1985	Total AAs negatively influenced male reproductive success (r = - 0.79).					
31020407	7	82	theme	different	1250:1258	arg1	populations					1260:1270	the different populations	1246:1270	the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05)	1246:1385	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	14	83	from	classes	2085:2091	arg1	taste					2067:2071	the AAs' taste	2058:2071	the AAs' taste	2058:2071	Pollinators of the investigated species were found to be sensitive to the AAs' taste, from taste classes I and IV.					
31020407	7	84	theme	low	1119:1121	arg1	concentrations					1129:1142	low sugar concentrations	1119:1142	low sugar concentrations (9.04-20.68%)	1119:1156	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	7	84	theme	low	1119:1121	arg1	%					1155:1155	9.04-20.68%	1145:1155	9.04-20.68%	1145:1155	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	8	85	theme	positive	1460:1467	arg1	selection					1469:1477	positive selection	1460:1477	positive selection on fructose content	1460:1497	Total sugar content did not influence reproductive success and we found positive selection on fructose content.					
31020407	1	86	theme	bifolia	258:264	arg1	nectar					236:241	the nectar	232:241	the nectar of Platanthera bifolia and P. chlorantha	232:282	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	12	87	theme	sugar	1776:1780	arg1	profiles					1789:1796	the sugar and AA profiles	1772:1796	profiles	1789:1796	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	10	88	theme	γ-aminobutyric	1585:1598	arg1	acid					1600:1603	γ-aminobutyric acid	1585:1603	γ-aminobutyric acid	1585:1603	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	12	89	theme	AA	1786:1787	arg1	profiles					1789:1796	the sugar and AA profiles	1772:1796	profiles	1789:1796	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	16	90	theme	available	2352:2360	arg1	data					2362:2365	the available data	2348:2365	the available data	2348:2365	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	3	91	theme	amino	401:405	arg1	AA					413:414	AA	413:414	AA	413:414	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	91	theme	amino	401:405	arg1	acid					407:410	sugar and amino acid	391:410	acid	407:410	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	92	theme	reproductive	593:604	arg1	success					606:612	reproductive success	593:612	reproductive success	593:612	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	93	from	composition	417:427	arg1	species					443:449	two sister species	432:449	two sister species	432:449	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	93	from	composition	417:427	arg1	bifolia					455:461	P. bifolia	452:461	P. bifolia	452:461	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	3	93	from	composition	417:427	arg1	chlorantha					470:479	P. chlorantha	467:479	P. chlorantha	467:479	Nectar sugar and amino acid (AA) composition in two sister species, P. bifolia and P. chlorantha, was analysed and the interspecies differences in nectar and the importance of these nectar characteristics for reproductive success were investigated.					
31020407	7	94	theme	bifolia	1084:1090	arg1	nectar					1071:1076	The nectar	1067:1076	The nectar of P. bifolia and P. chlorantha	1067:1108	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	16	95	theme	quality	2482:2488	arg1	importance					2461:2470	the importance	2457:2470	the importance of nectar quality for reproductive success of the studied species	2457:2536	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	12	96	from	populations	1855:1865	arg1	context					1874:1880	the context	1870:1880	the context of effectiveness of reproduction	1870:1913	To our knowledge, this is the first report that characterizes the sugar and AA profiles in the nectar of P. bifolia and P. chlorantha in natural populations in the context of effectiveness of reproduction.					
31020407	1	97	located	present	221:227	arg2	Sugars					121:126	Sugars	121:126	Sugars (glucose, fructose and sucrose)	121:158	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	97	located	present	221:227	arg2	glucose					129:135	glucose	129:135	glucose	129:135	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	97	located	present	221:227	arg2	fructose					138:145	fructose	138:145	fructose	138:145	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	97	located	present	221:227	arg2	acids					210:214	proteogenic and non-proteogenic amino acids	172:214	proteogenic and non-proteogenic amino acids	172:214	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	97	located	present	221:227	arg2	sucrose					151:157	sucrose	151:157	sucrose	151:157	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	1	97	located	present	221:227	arg1	nectar					236:241	the nectar	232:241	the nectar of Platanthera bifolia and P. chlorantha	232:282	Sugars (glucose, fructose and sucrose), as well as proteogenic and non-proteogenic amino acids, are present in the nectar of Platanthera bifolia and P. chlorantha.					
31020407	7	98	theme	hexoses	1202:1208	arg1	ratios					1210:1215	hexoses ratios	1202:1215	hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05)	1202:1385	The nectar of P. bifolia and P. chlorantha both had low sugar concentrations (9.04-20.68%) and were dominated by hexoses, with sucrose:hexoses ratios between 0.03 and 0.31 across the different populations (the average for the P. bifolia populations was 0.17 and the average for the P. chlorantha populations was - 0.05).					
31020407	9	99	located	detected	1534:1541	arg1	species					1563:1569	both Platanthera species	1546:1569	both Platanthera species	1546:1569	In general, 23 different AAs were detected in both Platanthera species.					
31020407	9	99	located	detected	1534:1541	arg1	general					1503:1509	general	1503:1509	general	1503:1509	In general, 23 different AAs were detected in both Platanthera species.					
31020407	9	99	located	detected	1534:1541	arg2	AAs					1525:1527	23 different AAs	1512:1527	23 different AAs	1512:1527	In general, 23 different AAs were detected in both Platanthera species.					
31020407	15	100	theme	reproductive	2128:2139	arg1	success					2141:2147	male reproductive success	2123:2147	male reproductive success	2123:2147	Correlation between male reproductive success and the content of AAs from these groups was 0.79 in both cases.					
31020407	15	101	theme	male	2123:2126	arg1	success					2141:2147	male reproductive success	2123:2147	male reproductive success	2123:2147	Correlation between male reproductive success and the content of AAs from these groups was 0.79 in both cases.					
31020407	16	102	theme	studied	2522:2528	arg1	species					2530:2536	the studied species	2518:2536	the studied species	2518:2536	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31020407	10	103	attach	present	1610:1616	arg2	acid					1600:1603	γ-aminobutyric acid	1585:1603	γ-aminobutyric acid	1585:1603	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	10	103	attach	present	1610:1616	arg1	population					1630:1639	only one population	1621:1639	only one population of P. chlorantha	1621:1656	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	10	103	attach	present	1610:1616	arg2	Cysteine					1572:1579	Cysteine	1572:1579	Cysteine	1572:1579	Cysteine and γ-aminobutyric acid were present in only one population of P. chlorantha.					
31020407	4	104	theme	NE	764:765	arg1	Poland					767:772	NE Poland	764:772	NE Poland	764:772	Nectar was collected from four P. bifolia and three P. chlorantha populations that exist in different habitats in three regions of NE Poland.					
31020407	16	105	theme	chlorantha	2291:2300	arg1	nectar					2302:2307	P. bifolia and P. chlorantha nectar	2273:2307	P. bifolia and P. chlorantha nectar	2273:2307	In this manuscript, we investigated the characteristics of P. bifolia and P. chlorantha nectar, and compared these characteristics to the available data in the context of their adaptations to the requirements of pollinators and with regard to the importance of nectar quality for reproductive success of the studied species.					
31075335	0	0	theme	tetracycline	105:116	arg1	removal					94:100	the removal	90:100	the removal of tetracycline from aqueous solution	90:138	Fabrication of MnFe2O4 nanoparticles embedded chitosan-diphenylureaformaldehyde resin for the removal of tetracycline from aqueous solution.					
31075335	0	1	theme	aqueous	123:129	arg1	solution					131:138	aqueous solution	123:138	aqueous solution	123:138	Fabrication of MnFe2O4 nanoparticles embedded chitosan-diphenylureaformaldehyde resin for the removal of tetracycline from aqueous solution.					
31075335	5	2	theme	fitting	1088:1094	arg1	R2					1121:1122	R2	1121:1122	R2	1121:1122	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	2	theme	fitting	1088:1094	arg1	0.9955					1126:1131	0.9955	1126:1131	0.9955	1126:1131	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	2	theme	fitting	1088:1094	arg1	coefficient					1108:1118	fitting correlation coefficient	1088:1118	fitting correlation coefficient (R2)	1088:1123	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	7	3	theme	porous	1340:1345	arg1	MF					1351:1352	the prepared porous CDF@MF	1327:1352	the prepared porous CDF@MF	1327:1352	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	7	3	theme	porous	1340:1345	arg1	adsorbent					1403:1411	adsorbent	1403:1411	adsorbent	1403:1411	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	7	4	theme	CDF	1347:1349	arg1	MF					1351:1352	the prepared porous CDF@MF	1327:1352	the prepared porous CDF@MF	1327:1352	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	7	4	theme	CDF	1347:1349	arg1	adsorbent					1403:1411	adsorbent	1403:1411	adsorbent	1403:1411	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	3	5	theme	antibiotic	692:701	arg1	removal					678:684	removal	678:684	removal of an antibiotic, namely, tetracycline (TC), from aqueous solution	678:751	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	3	6	theme	contact	841:847	arg1	time					849:852	the contact time	837:852	the contact time	837:852	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	1	7	theme	condensation	282:293	arg1	polymerization					295:308	condensation polymerization	282:308	condensation polymerization	282:308	In this study, a magnetic composite was synthesized using chitosan, diphenylurea and formaldehyde with magnetic nanoparticles, (MnFe2O4) via condensation polymerization.					
31075335	5	8	theme	Langmuir	1047:1054	arg1	adsorption					1056:1065	Langmuir adsorption	1047:1065	the Langmuir adsorption isotherm model	1043:1080	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	9	theme	adsorption	1056:1065	arg1	model					1076:1080	the Langmuir adsorption isotherm model	1043:1080	the Langmuir adsorption isotherm model	1043:1080	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	6	10	theme	adsorption	1191:1200	arg1	capacity					1202:1209	the adsorption capacity	1187:1209	the adsorption capacity	1187:1209	The regeneration study advice that no major change in the adsorption capacity was noticed and show 157.97% adsorption performance after seven cycles.					
31075335	2	11	theme	scanning	477:484	arg1	microscopy					495:504	scanning electron microscopy	477:504	scanning electron microscopy (SEM)	477:510	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	11	theme	scanning	477:484	arg1	SEM					507:509	SEM	507:509	SEM	507:509	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	5	12	theme	isotherm	1067:1074	arg1	model					1076:1080	the Langmuir adsorption isotherm model	1043:1080	the Langmuir adsorption isotherm model	1043:1080	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	6	13	theme	major	1171:1175	arg1	change					1177:1182	no major change	1168:1182	no major change in the adsorption capacity	1168:1209	The regeneration study advice that no major change in the adsorption capacity was noticed and show 157.97% adsorption performance after seven cycles.					
31075335	6	14	theme	adsorption	1240:1249	arg1	performance					1251:1261	157.97% adsorption performance	1232:1261	157.97% adsorption performance	1232:1261	The regeneration study advice that no major change in the adsorption capacity was noticed and show 157.97% adsorption performance after seven cycles.					
31075335	7	15	used	used	1363:1366	arg2	adsorbent					1403:1411	adsorbent	1403:1411	adsorbent	1403:1411	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	7	15	used	used	1363:1366	arg2	MF					1351:1352	the prepared porous CDF@MF	1327:1352	the prepared porous CDF@MF	1327:1352	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	4	16	theme	kinetics	870:877	arg1	results					879:885	The adsorption kinetics results	855:885	The adsorption kinetics results	855:885	The adsorption kinetics results exhibited that the adsorption process followed a pseudo-first-order model.					
31075335	7	17	theme	@	1350:1350	arg1	MF					1351:1352	the prepared porous CDF@MF	1327:1352	the prepared porous CDF@MF	1327:1352	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	7	17	theme	@	1350:1350	arg1	adsorbent					1403:1411	adsorbent	1403:1411	adsorbent	1403:1411	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	2	18	theme	analytical	397:406	arg1	Fourier					429:435	Fourier	429:435	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc	429:587	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	18	theme	analytical	397:406	arg1	techniques					408:417	several analytical techniques	389:417	several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc	389:587	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	6	19	theme	regeneration	1137:1148	arg1	study					1150:1154	The regeneration study	1133:1154	The regeneration study	1133:1154	The regeneration study advice that no major change in the adsorption capacity was noticed and show 157.97% adsorption performance after seven cycles.					
31075335	1	20	with	formaldehyde	226:237	arg1	nanoparticles					253:265	magnetic nanoparticles	244:265	magnetic nanoparticles	244:265	In this study, a magnetic composite was synthesized using chitosan, diphenylurea and formaldehyde with magnetic nanoparticles, (MnFe2O4) via condensation polymerization.					
31075335	2	21	theme	magnetic	333:340	arg1	MF					361:362	CDF@MF	357:362	CDF@MF	357:362	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	21	theme	magnetic	333:340	arg1	nanocomposite					342:354	The newly synthesized magnetic nanocomposite	311:354	The newly synthesized magnetic nanocomposite (CDF@MF)	311:363	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	0	22	theme	MnFe2O4	15:21	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of MnFe2O4	0:21	Fabrication of MnFe2O4 nanoparticles embedded chitosan-diphenylureaformaldehyde resin for the removal of tetracycline from aqueous solution.					
31075335	2	23	theme	X-ray	557:561	arg1	XRD					576:578	XRD	576:578	XRD	576:578	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	23	theme	X-ray	557:561	arg1	diffraction					563:573	X-ray diffraction	557:573	X-ray diffraction (XRD)	557:579	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	24	theme	synthesized	321:331	arg1	MF					361:362	CDF@MF	357:362	CDF@MF	357:362	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	24	theme	synthesized	321:331	arg1	nanocomposite					342:354	The newly synthesized magnetic nanocomposite	311:354	The newly synthesized magnetic nanocomposite (CDF@MF)	311:363	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	0	25	theme	chitosan-diphenylureaformaldehyde	46:78	arg1	resin					80:84	embedded chitosan-diphenylureaformaldehyde resin	37:84	embedded chitosan-diphenylureaformaldehyde resin for the removal of tetracycline from aqueous solution	37:138	Fabrication of MnFe2O4 nanoparticles embedded chitosan-diphenylureaformaldehyde resin for the removal of tetracycline from aqueous solution.					
31075335	1	26	with	diphenylurea	209:220	arg1	nanoparticles					253:265	magnetic nanoparticles	244:265	magnetic nanoparticles	244:265	In this study, a magnetic composite was synthesized using chitosan, diphenylurea and formaldehyde with magnetic nanoparticles, (MnFe2O4) via condensation polymerization.					
31075335	5	27	theme	maximum	966:972	arg1	168.24 mg/g					1022:1032	168.24 mg/g	1022:1032	168.24 mg/g based on the Langmuir adsorption isotherm model	1022:1080	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	27	theme	maximum	966:972	arg1	capacity					985:992	The maximum adsorption capacity	962:992	The maximum adsorption capacity for TC	962:999	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	28	with	168.24 mg/g	1022:1032	arg1	R2					1121:1122	R2	1121:1122	R2	1121:1122	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	28	with	168.24 mg/g	1022:1032	arg1	0.9955					1126:1131	0.9955	1126:1131	0.9955	1126:1131	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	28	with	168.24 mg/g	1022:1032	arg1	coefficient					1108:1118	fitting correlation coefficient	1088:1118	fitting correlation coefficient (R2)	1088:1123	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	0	29	theme	embedded	37:44	arg1	resin					80:84	embedded chitosan-diphenylureaformaldehyde resin	37:84	embedded chitosan-diphenylureaformaldehyde resin for the removal of tetracycline from aqueous solution	37:138	Fabrication of MnFe2O4 nanoparticles embedded chitosan-diphenylureaformaldehyde resin for the removal of tetracycline from aqueous solution.					
31075335	5	30	theme	correlation	1096:1106	arg1	R2					1121:1122	R2	1121:1122	R2	1121:1122	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	30	theme	correlation	1096:1106	arg1	0.9955					1126:1131	0.9955	1126:1131	0.9955	1126:1131	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	30	theme	correlation	1096:1106	arg1	coefficient					1108:1118	fitting correlation coefficient	1088:1118	fitting correlation coefficient (R2)	1088:1123	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	4	31	theme	adsorption	859:868	arg1	results					879:885	The adsorption kinetics results	855:885	The adsorption kinetics results	855:885	The adsorption kinetics results exhibited that the adsorption process followed a pseudo-first-order model.					
31075335	3	32	theme	@	629:629	arg1	MF					630:631	CDF@MF	626:631	CDF@MF	626:631	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	2	33	dep	transform	437:445	arg1	infrared					447:454	infrared	447:454	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc	437:587	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	3	34	theme	MF	630:631	arg1	applicability					609:621	the applicability	605:621	the applicability of CDF@MF	605:631	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	2	35	theme	electron	486:493	arg1	microscopy					495:504	scanning electron microscopy	477:504	scanning electron microscopy (SEM)	477:510	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	35	theme	electron	486:493	arg1	SEM					507:509	SEM	507:509	SEM	507:509	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	7	36	dep	low-cost	1373:1380	arg1	reusable					1394:1401	reusable	1394:1401	reusable	1394:1401	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	7	36	dep	low-cost	1373:1380	arg1	effective					1383:1391	effective	1383:1391	effective	1383:1391	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	3	37	from	solution	744:751	arg1	removal					678:684	removal	678:684	removal of an antibiotic, namely, tetracycline (TC), from aqueous solution	678:751	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	7	38	theme	wastewater	1432:1441	arg1	treatment					1443:1451	pharmaceutical wastewater treatment	1417:1451	pharmaceutical wastewater treatment	1417:1451	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	4	39	theme	adsorption	906:915	arg1	process					917:923	the adsorption process	902:923	the adsorption process	902:923	The adsorption kinetics results exhibited that the adsorption process followed a pseudo-first-order model.					
31075335	3	40	theme	adsorbent	791:799	arg1	dose					801:804	the adsorbent dose	787:804	the adsorbent dose	787:804	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	6	41	theme	%	1238:1238	arg1	performance					1251:1261	157.97% adsorption performance	1232:1261	157.97% adsorption performance	1232:1261	The regeneration study advice that no major change in the adsorption capacity was noticed and show 157.97% adsorption performance after seven cycles.					
31075335	0	42	from	solution	131:138	arg1	removal					94:100	the removal	90:100	the removal of tetracycline from aqueous solution	90:138	Fabrication of MnFe2O4 nanoparticles embedded chitosan-diphenylureaformaldehyde resin for the removal of tetracycline from aqueous solution.					
31075335	5	43	theme	adsorption	974:983	arg1	168.24 mg/g					1022:1032	168.24 mg/g	1022:1032	168.24 mg/g based on the Langmuir adsorption isotherm model	1022:1080	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	5	43	theme	adsorption	974:983	arg1	capacity					985:992	The maximum adsorption capacity	962:992	The maximum adsorption capacity for TC	962:999	The maximum adsorption capacity for TC was calculated to be 168.24 mg/g based on the Langmuir adsorption isotherm model, with fitting correlation coefficient (R2), 0.9955.					
31075335	2	44	theme	@	360:360	arg1	MF					361:362	CDF@MF	357:362	CDF@MF	357:362	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	44	theme	@	360:360	arg1	nanocomposite					342:354	The newly synthesized magnetic nanocomposite	311:354	The newly synthesized magnetic nanocomposite (CDF@MF)	311:363	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	7	45	theme	prepared	1331:1338	arg1	MF					1351:1352	the prepared porous CDF@MF	1327:1352	the prepared porous CDF@MF	1327:1352	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	7	45	theme	prepared	1331:1338	arg1	adsorbent					1403:1411	adsorbent	1403:1411	adsorbent	1403:1411	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	1	46	theme	magnetic	244:251	arg1	nanoparticles					253:265	magnetic nanoparticles	244:265	magnetic nanoparticles	244:265	In this study, a magnetic composite was synthesized using chitosan, diphenylurea and formaldehyde with magnetic nanoparticles, (MnFe2O4) via condensation polymerization.					
31075335	6	47	theme	157.97	1232:1237	arg1	%					1238:1238	%	1238:1238	%	1238:1238	The regeneration study advice that no major change in the adsorption capacity was noticed and show 157.97% adsorption performance after seven cycles.					
31075335	3	48	theme	CDF	626:628	arg1	MF					630:631	CDF@MF	626:631	CDF@MF	626:631	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	2	49	theme	CDF	357:359	arg1	MF					361:362	CDF@MF	357:362	CDF@MF	357:362	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	49	theme	CDF	357:359	arg1	nanocomposite					342:354	The newly synthesized magnetic nanocomposite	311:354	The newly synthesized magnetic nanocomposite (CDF@MF)	311:363	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	6	50	from	change	1177:1182	arg1	capacity					1202:1209	the adsorption capacity	1187:1209	the adsorption capacity	1187:1209	The regeneration study advice that no major change in the adsorption capacity was noticed and show 157.97% adsorption performance after seven cycles.					
31075335	3	51	theme	several	759:765	arg1	dose					801:804	the adsorbent dose	787:804	the adsorbent dose	787:804	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	3	51	theme	several	759:765	arg1	time					849:852	the contact time	837:852	the contact time	837:852	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	3	51	theme	several	759:765	arg1	concentration					819:831	the initial concentration	807:831	the initial concentration	807:831	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	3	51	theme	several	759:765	arg1	factors					767:773	several factors	759:773	several factors such as pH, the adsorbent dose, the initial concentration and the contact time	759:852	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	3	51	theme	several	759:765	arg1	pH					783:784	pH	783:784	pH	783:784	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	2	52	theme	electron	526:533	arg1	TEM					547:549	TEM	547:549	TEM	547:549	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	52	theme	electron	526:533	arg1	microscopy					535:544	transmission electron microscopy	513:544	transmission electron microscopy (TEM)	513:550	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	7	53	theme	pharmaceutical	1417:1430	arg1	treatment					1443:1451	pharmaceutical wastewater treatment	1417:1451	pharmaceutical wastewater treatment	1417:1451	These results are standing to indicate that the prepared porous CDF@MF could be used as a low-cost, effective, reusable adsorbent for pharmaceutical wastewater treatment.					
31075335	2	54	dep	Fourier	429:435	arg1	transform					437:445	transform	437:445	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc	437:587	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	55	theme	several	389:395	arg1	Fourier					429:435	Fourier	429:435	Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc	429:587	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	55	theme	several	389:395	arg1	techniques					408:417	several analytical techniques	389:417	several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc	389:587	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	3	56	theme	aqueous	736:742	arg1	solution					744:751	aqueous solution	736:751	aqueous solution	736:751	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31075335	2	57	theme	transmission	513:524	arg1	TEM					547:549	TEM	547:549	TEM	547:549	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	2	57	theme	transmission	513:524	arg1	microscopy					535:544	transmission electron microscopy	513:544	transmission electron microscopy (TEM)	513:550	The newly synthesized magnetic nanocomposite (CDF@MF) was characterized using several analytical techniques including Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD) and etc.					
31075335	4	58	theme	pseudo-first-order	936:953	arg1	model					955:959	a pseudo-first-order model	934:959	a pseudo-first-order model	934:959	The adsorption kinetics results exhibited that the adsorption process followed a pseudo-first-order model.					
31075335	1	59	theme	magnetic	158:165	arg1	composite					167:175	a magnetic composite	156:175	a magnetic composite	156:175	In this study, a magnetic composite was synthesized using chitosan, diphenylurea and formaldehyde with magnetic nanoparticles, (MnFe2O4) via condensation polymerization.					
31075335	3	60	theme	initial	811:817	arg1	concentration					819:831	the initial concentration	807:831	the initial concentration	807:831	To investigate the applicability of CDF@MF, it was employed as an adsorbent for rapidly removal of an antibiotic, namely, tetracycline (TC), from aqueous solution using several factors such as pH, the adsorbent dose, the initial concentration and the contact time.					
31006449	2	0	theme	pressure	491:498	arg1	level					500:504	moderate pressure level	482:504	moderate pressure level (0.1-300 MPa)	482:518	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	2	0	theme	pressure	491:498	arg1	0.1-300 MPa					507:517	0.1-300 MPa	507:517	0.1-300 MPa	507:517	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	3	1	theme	random	694:699	arg1	structures					662:671	other structures	656:671	other structures (β-sheet, β-turn and random coil)	656:705	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	1	theme	random	694:699	arg1	coil					701:704	random coil	694:704	random coil	694:704	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	1	2	theme	gelation	162:169	arg1	properties					171:180	the gelation properties	158:180	the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan	158:287	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	6	3	theme	immobilized	1009:1019	arg1	water					1021:1025	immobilized water	1009:1025	immobilized water of pressurised gels	1009:1045	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	2	4	theme	moderate	482:489	arg1	level					500:504	moderate pressure level	482:504	moderate pressure level (0.1-300 MPa)	482:518	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	2	4	theme	moderate	482:489	arg1	0.1-300 MPa					507:517	0.1-300 MPa	507:517	0.1-300 MPa	507:517	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	2	5	theme	konjac	350:355	arg1	glucomannan					357:367	Deacetylated konjac glucomannan	337:367	Deacetylated konjac glucomannan	337:367	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	3	6	dep	structures	662:671	arg1	structures					662:671	other structures	656:671	other structures (β-sheet, β-turn and random coil)	656:705	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	6	dep	structures	662:671	arg1	β-turn					683:688	β-turn	683:688	β-turn	683:688	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	6	dep	structures	662:671	arg1	coil					701:704	random coil	694:704	random coil	694:704	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	6	dep	structures	662:671	arg1	β-sheet					674:680	β-sheet	674:680	β-sheet	674:680	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	0	7	from	Effects	0:6	arg1	forces					73:78	molecular forces	63:78	molecular forces	63:78	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	0	7	from	Effects	0:6	arg1	properties					48:57	gelation properties	39:57	gelation properties	39:57	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	0	8	theme	myosin	83:88	arg1	forces					73:78	molecular forces	63:78	molecular forces	63:78	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	0	8	theme	myosin	83:88	arg1	properties					48:57	gelation properties	39:57	gelation properties	39:57	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	4	9	theme	storage	749:755	arg1	modulus					757:763	storage modulus	749:763	storage modulus of the pressurised myosin	749:789	Dynamic rheological tests indicated that storage modulus of the pressurised myosin was increased to maximum under 100 MPa.					
31006449	1	10	theme	deacetylated	257:268	arg1	glucomannan					277:287	2% (w/v) deacetylated konjac glucomannan	248:287	2% (w/v) deacetylated konjac glucomannan	248:287	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	0	11	theme	konjac	114:119	arg1	glucomannan					121:131	deacetylated konjac glucomannan	101:131	deacetylated konjac glucomannan	101:131	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	5	12	theme	compact	907:913	arg1	microstructure					925:938	a compact networked microstructure	905:938	a compact networked microstructure	905:938	Scanning electron microscopy images revealed that HPP at 300 MPa produced a compact networked microstructure.					
31006449	6	13	from	increase	997:1004	arg1	water					1021:1025	immobilized water	1009:1025	immobilized water of pressurised gels	1009:1045	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	5	14	theme	Scanning	831:838	arg1	microscopy					849:858	Scanning electron microscopy	831:858	Scanning electron microscopy images	831:865	Scanning electron microscopy images revealed that HPP at 300 MPa produced a compact networked microstructure.					
31006449	6	15	theme	gel	1212:1214	arg1	strength					1216:1223	gel strength	1212:1223	gel strength	1212:1223	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	6	16	theme	hydrogen	1081:1088	arg1	bonds					1090:1094	enhanced hydrogen bonds	1072:1094	enhanced hydrogen bonds	1072:1094	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	6	17	theme	hydrophobic	1150:1160	arg1	interactions					1162:1173	the HPP-induced hydrophobic interactions	1134:1173	the HPP-induced hydrophobic interactions	1134:1173	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	0	18	theme	deacetylated	101:112	arg1	glucomannan					121:131	deacetylated konjac glucomannan	101:131	deacetylated konjac glucomannan	101:131	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	2	19	theme	gel	419:421	arg1	strength					423:430	the gel strength	415:430	the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa)	415:574	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	6	20	theme	HPP-induced	1138:1148	arg1	interactions					1162:1173	the HPP-induced hydrophobic interactions	1134:1173	the HPP-induced hydrophobic interactions	1134:1173	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	1	21	theme	composite	185:193	arg1	gels					195:198	composite gels	185:198	composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan	185:287	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	5	22	dep	microscopy	849:858	arg1	images					860:865	images	860:865	images	860:865	Scanning electron microscopy images revealed that HPP at 300 MPa produced a compact networked microstructure.					
31006449	1	23	theme	gels	195:198	arg1	properties					171:180	the gelation properties	158:180	the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan	158:287	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	4	24	theme	pressurised	772:782	arg1	myosin					784:789	the pressurised myosin	768:789	the pressurised myosin	768:789	Dynamic rheological tests indicated that storage modulus of the pressurised myosin was increased to maximum under 100 MPa.					
31006449	1	25	theme	konjac	270:275	arg1	glucomannan					277:287	2% (w/v) deacetylated konjac glucomannan	248:287	2% (w/v) deacetylated konjac glucomannan	248:287	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	4	26	theme	rheological	716:726	arg1	tests					728:732	Dynamic rheological tests	708:732	Dynamic rheological tests	708:732	Dynamic rheological tests indicated that storage modulus of the pressurised myosin was increased to maximum under 100 MPa.					
31006449	2	27	theme	immobilized	383:393	arg1	water					395:399	the immobilized water	379:399	the immobilized water	379:399	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	0	28	theme	pressure	16:23	arg1	processing					25:34	high pressure processing	11:34	high pressure processing	11:34	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	5	29	theme	electron	840:847	arg1	microscopy					849:858	Scanning electron microscopy	831:858	Scanning electron microscopy images	831:865	Scanning electron microscopy images revealed that HPP at 300 MPa produced a compact networked microstructure.					
31006449	5	30	from	300 MPa	888:894	arg1	at					885:886	HPP at 300 MPa	881:894	HPP at 300 MPa	881:894	Scanning electron microscopy images revealed that HPP at 300 MPa produced a compact networked microstructure.					
31006449	5	30	from	300 MPa	888:894	arg1	HPP					881:883	HPP at 300 MPa	881:894	HPP at 300 MPa	881:894	Scanning electron microscopy images revealed that HPP at 300 MPa produced a compact networked microstructure.					
31006449	6	31	theme	dominating	1180:1189	arg1	mechanism					1191:1199	dominating mechanism	1180:1199	dominating mechanism	1180:1199	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	0	32	theme	high	11:14	arg1	processing					25:34	high pressure processing	11:34	high pressure processing	11:34	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	2	33	theme	pressure	547:554	arg1	400-500 MPa					563:573	400-500 MPa	563:573	400-500 MPa	563:573	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	2	33	theme	pressure	547:554	arg1	level					556:560	strong pressure level	540:560	strong pressure level (400-500 MPa)	540:574	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	3	34	theme	α-helix	643:649	arg1	unfolding					589:597	unfolding	589:597	unfolding of myosin	589:607	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	34	theme	α-helix	643:649	arg1	transformation					625:638	the partial transformation	613:638	the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil)	613:705	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	6	35	theme	electrostatic	1100:1112	arg1	interactions					1114:1125	electrostatic interactions	1100:1125	electrostatic interactions	1100:1125	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	1	36	theme	golden	214:219	arg1	myosin					237:242	golden threadfin bream myosin	214:242	golden threadfin bream myosin	214:242	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	1	37	theme	different	295:303	arg1	HPP					331:333	HPP	331:333	HPP	331:333	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	1	37	theme	different	295:303	arg1	processing					319:328	different high pressure processing	295:328	different high pressure processing (HPP)	295:334	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	2	38	theme	strong	540:545	arg1	400-500 MPa					563:573	400-500 MPa	563:573	400-500 MPa	563:573	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	2	38	theme	strong	540:545	arg1	level					556:560	strong pressure level	540:560	strong pressure level (400-500 MPa)	540:574	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	1	39	dep	%	249:249	arg1	w/v					252:254	w/v	252:254	w/v	252:254	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	6	40	theme	forces	965:970	arg1	analysis					972:979	the molecular forces analysis	951:979	the molecular forces analysis	951:979	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	5	41	theme	HPP	881:883	arg1	300 MPa					888:894	HPP at 300 MPa	881:894	HPP at 300 MPa	881:894	Scanning electron microscopy images revealed that HPP at 300 MPa produced a compact networked microstructure.					
31006449	1	42	theme	threadfin	221:229	arg1	myosin					237:242	golden threadfin bream myosin	214:242	golden threadfin bream myosin	214:242	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	0	43	theme	processing	25:34	arg1	Effects					0:6	Effects	0:6	Effects of high pressure processing on gelation properties and molecular forces of myosin	0:88	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	1	44	theme	high	305:308	arg1	HPP					331:333	HPP	331:333	HPP	331:333	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	1	44	theme	high	305:308	arg1	processing					319:328	different high pressure processing	295:328	different high pressure processing (HPP)	295:334	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	2	45	theme	gel	442:444	arg1	strength					423:430	the gel strength	415:430	the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa)	415:574	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	1	46	theme	bream	231:235	arg1	myosin					237:242	golden threadfin bream myosin	214:242	golden threadfin bream myosin	214:242	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	1	47	theme	pressure	310:317	arg1	HPP					331:333	HPP	331:333	HPP	331:333	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	1	47	theme	pressure	310:317	arg1	processing					319:328	different high pressure processing	295:328	different high pressure processing (HPP)	295:334	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	2	48	theme	Deacetylated	337:348	arg1	glucomannan					357:367	Deacetylated konjac glucomannan	337:367	Deacetylated konjac glucomannan	337:367	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	0	49	theme	gelation	39:46	arg1	properties					48:57	gelation properties	39:57	gelation properties	39:57	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	6	50	theme	enhanced	1072:1079	arg1	bonds					1090:1094	enhanced hydrogen bonds	1072:1094	enhanced hydrogen bonds	1072:1094	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	5	51	theme	networked	915:923	arg1	microstructure					925:938	a compact networked microstructure	905:938	a compact networked microstructure	905:938	Scanning electron microscopy images revealed that HPP at 300 MPa produced a compact networked microstructure.					
31006449	6	52	theme	molecular	955:963	arg1	analysis					972:979	the molecular forces analysis	951:979	the molecular forces analysis	951:979	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	4	53	theme	myosin	784:789	arg1	modulus					757:763	storage modulus	749:763	storage modulus of the pressurised myosin	749:789	Dynamic rheological tests indicated that storage modulus of the pressurised myosin was increased to maximum under 100 MPa.					
31006449	4	54	theme	Dynamic	708:714	arg1	tests					728:732	Dynamic rheological tests	708:732	Dynamic rheological tests	708:732	Dynamic rheological tests indicated that storage modulus of the pressurised myosin was increased to maximum under 100 MPa.					
31006449	6	55	theme	gels	1042:1045	arg1	water					1021:1025	immobilized water	1009:1025	immobilized water of pressurised gels	1009:1045	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	3	56	theme	other	656:660	arg1	structures					662:671	other structures	656:671	other structures (β-sheet, β-turn and random coil)	656:705	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	56	theme	other	656:660	arg1	β-turn					683:688	β-turn	683:688	β-turn	683:688	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	56	theme	other	656:660	arg1	coil					701:704	random coil	694:704	random coil	694:704	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	56	theme	other	656:660	arg1	β-sheet					674:680	β-sheet	674:680	β-sheet	674:680	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	57	theme	partial	617:623	arg1	transformation					625:638	the partial transformation	613:638	the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil)	613:705	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	6	58	theme	pressurised	1030:1040	arg1	gels					1042:1045	pressurised gels	1030:1045	pressurised gels	1030:1045	Moreover, the molecular forces analysis proved that the increase in immobilized water of pressurised gels was mainly attributed to enhanced hydrogen bonds and electrostatic interactions, while the HPP-induced hydrophobic interactions were dominating mechanism to improve gel strength.					
31006449	0	59	theme	molecular	63:71	arg1	forces					73:78	molecular forces	63:78	molecular forces	63:78	Effects of high pressure processing on gelation properties and molecular forces of myosin containing deacetylated konjac glucomannan.					
31006449	3	60	theme	myosin	602:607	arg1	unfolding					589:597	unfolding	589:597	unfolding of myosin	589:607	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	3	60	theme	myosin	602:607	arg1	transformation					625:638	the partial transformation	613:638	the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil)	613:705	HPP induced unfolding of myosin and the partial transformation of α-helix into other structures (β-sheet, β-turn and random coil).					
31006449	5	61	theme	at	885:886	arg1	300 MPa					888:894	HPP at 300 MPa	881:894	HPP at 300 MPa	881:894	Scanning electron microscopy images revealed that HPP at 300 MPa produced a compact networked microstructure.					
31006449	1	62	theme	2	248:248	arg1	%					249:249	%	249:249	%	249:249	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
31006449	2	63	theme	myosin	435:440	arg1	gel					442:444	myosin gel	435:444	myosin gel	435:444	Deacetylated konjac glucomannan increased the immobilized water and increased the gel strength of myosin gel, which were further increased under moderate pressure level (0.1-300 MPa) but decreased under strong pressure level (400-500 MPa).					
31006449	1	64	theme	%	249:249	arg1	glucomannan					277:287	2% (w/v) deacetylated konjac glucomannan	248:287	2% (w/v) deacetylated konjac glucomannan	248:287	This study investigated the gelation properties of composite gels prepared with golden threadfin bream myosin and 2% (w/v) deacetylated konjac glucomannan under different high pressure processing (HPP).					
30940007	0	0	theme	cell	111:114	arg1	culture					116:122	three-dimensional cell culture	93:122	three-dimensional cell culture	93:122	Nanocellulose/PEGDA aerogel scaffolds with tunable modulus prepared by stereolithography for three-dimensional cell culture.					
30940007	3	1	theme	CNFs/PEGDA	542:551	arg1	scaffold					562:569	3D cytocompatibility CNFs/PEGDA hydrogel scaffold	521:569	3D cytocompatibility CNFs/PEGDA hydrogel scaffold	521:569	Then the CNFs/PEGDA mixture was used to fabricate 3D cytocompatibility CNFs/PEGDA hydrogel scaffold by stereolithograph（SLA）process.					
30940007	0	2	theme	three-dimensional	93:109	arg1	culture					116:122	three-dimensional cell culture	93:122	three-dimensional cell culture	93:122	Nanocellulose/PEGDA aerogel scaffolds with tunable modulus prepared by stereolithography for three-dimensional cell culture.					
30940007	9	3	theme	tissue	1362:1367	arg1	scaffolds					1381:1389	tissue engineering scaffolds	1362:1389	tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation	1362:1479	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	9	4	theme	superior	1335:1342	arg1	method					1344:1349	a superior method	1333:1349	a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation	1333:1479	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	6	5	theme	fabricated	889:898	arg1	scaffolds					919:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds	881:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds	881:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	3	6	theme	hydrogel	553:560	arg1	scaffold					562:569	3D cytocompatibility CNFs/PEGDA hydrogel scaffold	521:569	3D cytocompatibility CNFs/PEGDA hydrogel scaffold	521:569	Then the CNFs/PEGDA mixture was used to fabricate 3D cytocompatibility CNFs/PEGDA hydrogel scaffold by stereolithograph（SLA）process.					
30940007	5	7	theme	CNFs	792:795	arg1	contents					797:804	different CNFs contents	782:804	different CNFs contents	782:804	The results showed that the CNFs/PEGDA mixtures with different CNFs contents are all transparent, homogeneous and with obvious shear-thinning property.					
30940007	9	8	theme	engineering	1369:1379	arg1	scaffolds					1381:1389	tissue engineering scaffolds	1362:1389	tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation	1362:1479	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	4	9	theme	hydrogels	718:726	arg1	freeze-drying					701:713	the freeze-drying	697:713	the freeze-drying of hydrogels	697:726	The CNFs/PEGDA hydrogels were shaped by SLA, and then the aerogel scaffolds were prepared by the freeze-drying of hydrogels.					
30940007	8	10	theme	CNFs/PEGDA	1215:1224	arg1	materials					1226:1234	the CNFs/PEGDA materials	1211:1234	the CNFs/PEGDA materials	1211:1234	In addition, the NIH 3T3 cells tightly adhere on the CNFs/PEGDA materials and spread on the scaffolds with good differentiation and viability.					
30940007	8	11	with	scaffolds	1254:1262	arg1	differentiation					1274:1288	good differentiation	1269:1288	good differentiation	1269:1288	In addition, the NIH 3T3 cells tightly adhere on the CNFs/PEGDA materials and spread on the scaffolds with good differentiation and viability.					
30940007	8	11	with	scaffolds	1254:1262	arg1	viability					1294:1302	viability	1294:1302	viability	1294:1302	In addition, the NIH 3T3 cells tightly adhere on the CNFs/PEGDA materials and spread on the scaffolds with good differentiation and viability.					
30940007	6	12	theme	high	978:981	arg1	porosity					983:990	high porosity	978:990	high porosity of approximately 90%	978:1011	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	2	13	theme	photopolymerizable	429:446	arg1	resin					456:460	waterborne photopolymerizable acrylic resin	418:460	a waterborne photopolymerizable acrylic resin (PEGDA)	416:468	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	13	theme	photopolymerizable	429:446	arg1	PEGDA					463:467	PEGDA	463:467	PEGDA	463:467	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	14	theme	CNFs/PEGDA	326:335	arg1	mixture					338:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	14	theme	CNFs/PEGDA	326:335	arg1	material					360:367	a novelty 3D material	347:367	a novelty 3D material	347:367	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	1	15	theme	significant	200:210	arg1	attention					212:220	significant attention	200:220	significant attention	200:220	Three-dimensional (3D) porous scaffolds made of biopolymers have attracted significant attention in tissue engineering applications.					
30940007	9	16	theme	suitable	1407:1414	arg1	strength					1427:1434	suitable mechanical strength	1407:1434	suitable mechanical strength	1407:1434	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	7	17	theme	porous	1120:1125	arg1	structure					1127:1135	porous structure	1120:1135	porous structure	1120:1135	It is found that CNFs in the composite scaffolds played a significant role in structural shape integrity, porous structure and mechanical strength.					
30940007	0	18	theme	Nanocellulose/PEGDA	0:18	arg1	aerogel					20:26	Nanocellulose/PEGDA aerogel	0:26	Nanocellulose/PEGDA aerogel	0:26	Nanocellulose/PEGDA aerogel scaffolds with tunable modulus prepared by stereolithography for three-dimensional cell culture.					
30940007	9	19	theme	mechanical	1416:1425	arg1	strength					1427:1434	suitable mechanical strength	1407:1434	suitable mechanical strength	1407:1434	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	6	20	theme	CNFs/PEGDA	900:909	arg1	scaffolds					919:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds	881:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds	881:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	9	21	theme	cell	1464:1467	arg1	cultivation					1469:1479	3D cell cultivation	1461:1479	3D cell cultivation	1461:1479	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	2	22	theme	3D	357:358	arg1	mixture					338:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	22	theme	3D	357:358	arg1	material					360:367	a novelty 3D material	347:367	a novelty 3D material	347:367	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	5	23	theme	CNFs/PEGDA	757:766	arg1	mixtures					768:775	the CNFs/PEGDA mixtures	753:775	the CNFs/PEGDA mixtures with different CNFs contents	753:804	The results showed that the CNFs/PEGDA mixtures with different CNFs contents are all transparent, homogeneous and with obvious shear-thinning property.					
30940007	5	23	theme	CNFs/PEGDA	757:766	arg1	transparent					814:824	transparent	814:824	transparent	814:824	The results showed that the CNFs/PEGDA mixtures with different CNFs contents are all transparent, homogeneous and with obvious shear-thinning property.					
30940007	3	24	used	used	503:506	arg2	mixture					491:497	Then the CNFs/PEGDA mixture	471:497	Then the CNFs/PEGDA mixture	471:497	Then the CNFs/PEGDA mixture was used to fabricate 3D cytocompatibility CNFs/PEGDA hydrogel scaffold by stereolithograph（SLA）process.					
30940007	9	25	contain	possesses	1397:1405	arg2	biocompatibility					1440:1455	biocompatibility	1440:1455	biocompatibility	1440:1455	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	9	25	contain	possesses	1397:1405	arg2	strength					1427:1434	suitable mechanical strength	1407:1434	suitable mechanical strength	1407:1434	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	9	25	contain	possesses	1397:1405	arg1	scaffolds					1381:1389	tissue engineering scaffolds	1362:1389	tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation	1362:1479	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	1	26	theme	tissue	225:230	arg1	applications					244:255	tissue engineering applications	225:255	tissue engineering applications	225:255	Three-dimensional (3D) porous scaffolds made of biopolymers have attracted significant attention in tissue engineering applications.					
30940007	6	27	theme	compressive	954:964	arg1	modulus					966:972	high and tunable compressive modulus	937:972	high and tunable compressive modulus	937:972	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	6	28	contain	possess	929:935	arg1	scaffolds					919:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds	881:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds	881:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	6	28	contain	possess	929:935	arg2	modulus					966:972	high and tunable compressive modulus	937:972	high and tunable compressive modulus	937:972	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	6	28	contain	possess	929:935	arg2	porosity					983:990	high porosity	978:990	high porosity of approximately 90%	978:1011	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	6	29	dep	fabricated	889:898	arg1	SLA					885:887	SLA	885:887	SLA	885:887	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	1	30	theme	engineering	232:242	arg1	applications					244:255	tissue engineering applications	225:255	tissue engineering applications	225:255	Three-dimensional (3D) porous scaffolds made of biopolymers have attracted significant attention in tissue engineering applications.					
30940007	6	31	theme	tunable	946:952	arg1	modulus					966:972	high and tunable compressive modulus	937:972	high and tunable compressive modulus	937:972	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	1	32	theme	Three-dimensional	125:141	arg1	scaffolds					155:163	Three-dimensional (3D) porous scaffolds	125:163	Three-dimensional (3D) porous scaffolds made of biopolymers	125:183	Three-dimensional (3D) porous scaffolds made of biopolymers have attracted significant attention in tissue engineering applications.					
30940007	3	33	theme	CNFs/PEGDA	480:489	arg1	mixture					491:497	Then the CNFs/PEGDA mixture	471:497	Then the CNFs/PEGDA mixture	471:497	Then the CNFs/PEGDA mixture was used to fabricate 3D cytocompatibility CNFs/PEGDA hydrogel scaffold by stereolithograph（SLA）process.					
30940007	0	34	theme	tunable	43:49	arg1	modulus					51:57	tunable modulus	43:57	tunable modulus prepared by stereolithography for three-dimensional cell culture	43:122	Nanocellulose/PEGDA aerogel scaffolds with tunable modulus prepared by stereolithography for three-dimensional cell culture.					
30940007	5	35	theme	obvious	848:854	arg1	property					871:878	obvious shear-thinning property	848:878	obvious shear-thinning property	848:878	The results showed that the CNFs/PEGDA mixtures with different CNFs contents are all transparent, homogeneous and with obvious shear-thinning property.					
30940007	2	36	theme	acrylic	448:454	arg1	resin					456:460	waterborne photopolymerizable acrylic resin	418:460	a waterborne photopolymerizable acrylic resin (PEGDA)	416:468	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	36	theme	acrylic	448:454	arg1	PEGDA					463:467	PEGDA	463:467	PEGDA	463:467	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	5	37	theme	shear-thinning	856:869	arg1	property					871:878	obvious shear-thinning property	848:878	obvious shear-thinning property	848:878	The results showed that the CNFs/PEGDA mixtures with different CNFs contents are all transparent, homogeneous and with obvious shear-thinning property.					
30940007	9	38	theme	3D	1461:1462	arg1	cultivation					1469:1479	3D cell cultivation	1461:1479	3D cell cultivation	1461:1479	These results have revealed a superior method to prepare tissue engineering scaffolds which possesses suitable mechanical strength and biocompatibility for 3D cell cultivation.					
30940007	8	39	theme	NIH	1179:1181	arg1	cells					1187:1191	the NIH 3T3 cells	1175:1191	the NIH 3T3 cells	1175:1191	In addition, the NIH 3T3 cells tightly adhere on the CNFs/PEGDA materials and spread on the scaffolds with good differentiation and viability.					
30940007	6	40	theme	high	937:940	arg1	modulus					966:972	high and tunable compressive modulus	937:972	high and tunable compressive modulus	937:972	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	7	41	theme	structural	1092:1101	arg1	integrity					1109:1117	structural shape integrity	1092:1117	structural shape integrity	1092:1117	It is found that CNFs in the composite scaffolds played a significant role in structural shape integrity, porous structure and mechanical strength.					
30940007	3	42	theme	3D	521:522	arg1	scaffold					562:569	3D cytocompatibility CNFs/PEGDA hydrogel scaffold	521:569	3D cytocompatibility CNFs/PEGDA hydrogel scaffold	521:569	Then the CNFs/PEGDA mixture was used to fabricate 3D cytocompatibility CNFs/PEGDA hydrogel scaffold by stereolithograph（SLA）process.					
30940007	5	43	theme	different	782:790	arg1	contents					797:804	different CNFs contents	782:804	different CNFs contents	782:804	The results showed that the CNFs/PEGDA mixtures with different CNFs contents are all transparent, homogeneous and with obvious shear-thinning property.					
30940007	4	44	theme	CNFs/PEGDA	608:617	arg1	hydrogels					619:627	The CNFs/PEGDA hydrogels	604:627	The CNFs/PEGDA hydrogels	604:627	The CNFs/PEGDA hydrogels were shaped by SLA, and then the aerogel scaffolds were prepared by the freeze-drying of hydrogels.					
30940007	7	45	theme	mechanical	1141:1150	arg1	strength					1152:1159	mechanical strength	1141:1159	mechanical strength	1141:1159	It is found that CNFs in the composite scaffolds played a significant role in structural shape integrity, porous structure and mechanical strength.					
30940007	7	46	theme	significant	1072:1082	arg1	role					1084:1087	a significant role	1070:1087	a significant role	1070:1087	It is found that CNFs in the composite scaffolds played a significant role in structural shape integrity, porous structure and mechanical strength.					
30940007	3	47	theme	cytocompatibility	524:540	arg1	scaffold					562:569	3D cytocompatibility CNFs/PEGDA hydrogel scaffold	521:569	3D cytocompatibility CNFs/PEGDA hydrogel scaffold	521:569	Then the CNFs/PEGDA mixture was used to fabricate 3D cytocompatibility CNFs/PEGDA hydrogel scaffold by stereolithograph（SLA）process.					
30940007	7	48	theme	shape	1103:1107	arg1	integrity					1109:1117	structural shape integrity	1092:1117	structural shape integrity	1092:1117	It is found that CNFs in the composite scaffolds played a significant role in structural shape integrity, porous structure and mechanical strength.					
30940007	1	49	theme	porous	148:153	arg1	scaffolds					155:163	Three-dimensional (3D) porous scaffolds	125:163	Three-dimensional (3D) porous scaffolds made of biopolymers	125:183	Three-dimensional (3D) porous scaffolds made of biopolymers have attracted significant attention in tissue engineering applications.					
30940007	2	50	theme	novelty	349:355	arg1	mixture					338:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	50	theme	novelty	349:355	arg1	material					360:367	a novelty 3D material	347:367	a novelty 3D material	347:367	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	4	51	theme	aerogel	662:668	arg1	scaffolds					670:678	the aerogel scaffolds	658:678	the aerogel scaffolds	658:678	The CNFs/PEGDA hydrogels were shaped by SLA, and then the aerogel scaffolds were prepared by the freeze-drying of hydrogels.					
30940007	8	52	theme	3T3	1183:1185	arg1	cells					1187:1191	the NIH 3T3 cells	1175:1191	the NIH 3T3 cells	1175:1191	In addition, the NIH 3T3 cells tightly adhere on the CNFs/PEGDA materials and spread on the scaffolds with good differentiation and viability.					
30940007	6	53	theme	aerogel	911:917	arg1	scaffolds					919:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds	881:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds	881:927	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	2	54	theme	diacrylate	314:323	arg1	mixture					338:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	54	theme	diacrylate	314:323	arg1	material					360:367	a novelty 3D material	347:367	a novelty 3D material	347:367	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	8	55	theme	good	1269:1272	arg1	differentiation					1274:1288	good differentiation	1269:1288	good differentiation	1269:1288	In addition, the NIH 3T3 cells tightly adhere on the CNFs/PEGDA materials and spread on the scaffolds with good differentiation and viability.					
30940007	5	56	with	mixtures	768:775	arg1	contents					797:804	different CNFs contents	782:804	different CNFs contents	782:804	The results showed that the CNFs/PEGDA mixtures with different CNFs contents are all transparent, homogeneous and with obvious shear-thinning property.					
30940007	2	57	theme	glycol	307:312	arg1	mixture					338:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	57	theme	glycol	307:312	arg1	material					360:367	a novelty 3D material	347:367	a novelty 3D material	347:367	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	1	58	dep	Three-dimensional	125:141	arg1	3D					144:145	3D	144:145	3D	144:145	Three-dimensional (3D) porous scaffolds made of biopolymers have attracted significant attention in tissue engineering applications.					
30940007	2	59	theme	waterborne	418:427	arg1	resin					456:460	waterborne photopolymerizable acrylic resin	418:460	a waterborne photopolymerizable acrylic resin (PEGDA)	416:468	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	59	theme	waterborne	418:427	arg1	PEGDA					463:467	PEGDA	463:467	PEGDA	463:467	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	6	60	theme	%	1011:1011	arg1	modulus					966:972	high and tunable compressive modulus	937:972	high and tunable compressive modulus	937:972	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	6	60	theme	%	1011:1011	arg1	porosity					983:990	high porosity	978:990	high porosity of approximately 90%	978:1011	The SLA fabricated CNFs/PEGDA aerogel scaffolds possess high and tunable compressive modulus and high porosity of approximately 90%.					
30940007	2	61	theme	cellulose-nanofibers/polyethylene	273:305	arg1	mixture					338:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture	273:344	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	2	61	theme	cellulose-nanofibers/polyethylene	273:305	arg1	material					360:367	a novelty 3D material	347:367	a novelty 3D material	347:367	In this study, cellulose-nanofibers/polyethylene glycol diacrylate (CNFs/PEGDA) mixture, a novelty 3D material, was prepared by physical mixing the CNFs with a waterborne photopolymerizable acrylic resin (PEGDA).					
30940007	7	62	theme	composite	1043:1051	arg1	scaffolds					1053:1061	the composite scaffolds	1039:1061	the composite scaffolds	1039:1061	It is found that CNFs in the composite scaffolds played a significant role in structural shape integrity, porous structure and mechanical strength.					
30940007	7	63	from	CNFs	1031:1034	arg1	scaffolds					1053:1061	the composite scaffolds	1039:1061	the composite scaffolds	1039:1061	It is found that CNFs in the composite scaffolds played a significant role in structural shape integrity, porous structure and mechanical strength.					
30415847	13	0	theme	BMRSS	2449:2453	arg1	diets					2463:2467	BMRSS and CCS diets	2449:2467	BMRSS and CCS diets	2449:2467	Overall, lactation performance improved when cows were fed diets formulated with BMRSS compared with CSS, but performance was not different for cows fed BMRSS and CCS diets.					
30415847	9	1	theme	fat	1614:1616	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	1	theme	fat	1614:1616	arg1	yield					1618:1622	milk fat yield	1609:1622	milk fat yield (0.08 kg/d)	1609:1634	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	1	theme	fat	1614:1616	arg1	kg/d					1630:1633	0.08 kg/d	1625:1633	0.08 kg/d	1625:1633	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	14	2	theme	sample	2489:2494	arg1	size					2496:2499	the small sample size	2479:2499	the small sample size	2479:2499	However, the small sample size may have influenced these results by increasing the margin of the error and, concurrently, the power of the meta-analysis.					
30415847	13	3	theme	CCS	2459:2461	arg1	diets					2463:2467	BMRSS and CCS diets	2449:2467	BMRSS and CCS diets	2449:2467	Overall, lactation performance improved when cows were fed diets formulated with BMRSS compared with CSS, but performance was not different for cows fed BMRSS and CCS diets.					
30415847	2	4	theme	means	485:489	arg1	comparisons					491:501	7 means comparisons	483:501	7 means comparisons	483:501	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	2	4	theme	means	485:489	arg1	CSS					478:480	CSS	478:480	CSS (7 means comparisons; 104 cows)	478:512	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	1	5	theme	conventional	245:256	arg1	CCS					271:273	CCS	271:273	CCS	271:273	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	5	theme	conventional	245:256	arg1	silage					263:268	conventional corn silage	245:268	conventional corn silage (CCS)	245:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	5	theme	conventional	245:256	arg1	cows					203:206	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	11	6	from	days	1962:1965	arg1	milk					1970:1973	milk	1970:1973	milk	1970:1973	Meta-regression indicated that days in milk affected DMI and milk production when CSS diets were compared with BMRSS diets, and DMI when CCS diets were compared with BMRSS diets.					
30415847	10	7	theme	lactose	1904:1910	arg1	yield					1912:1916	lactose yield	1904:1916	lactose yield (0.08 kg/d)	1904:1928	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	10	7	theme	lactose	1904:1910	arg1	kg/d					1924:1927	0.08 kg/d	1919:1927	0.08 kg/d	1919:1927	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	6	8	theme	milk	1001:1004	arg1	composition					1006:1016	milk composition	1001:1016	milk composition	1001:1016	Estimated effect size was calculated for DMI, milk production, and milk composition.					
30415847	10	9	theme	CCS	1758:1760	arg1	diets					1762:1766	CCS diets	1758:1766	CCS diets	1758:1766	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	8	10	theme	highest	1093:1099	arg1	degree					1101:1106	The highest degree	1089:1106	The highest degree of heterogeneity (I2 = 41.5 and 72.6%)	1089:1145	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	3	11	theme	fixed	615:619	arg1	models					639:644	fixed or random effects models	615:644	fixed or random effects models with the Metafor package of R (https://www.R-project.org)	615:702	Statistical analysis was performed using fixed or random effects models with the Metafor package of R (https://www.R-project.org).					
30415847	2	12	theme	contrast	458:465	arg1	diets					467:471	contrast diets	458:471	contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows)	458:552	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	8	13	contain	had	1324:1326	arg1	production					1313:1322	milk production	1308:1322	milk production	1308:1322	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	8	13	contain	had	1324:1326	arg2	degree					1339:1344	the lowest degree	1328:1344	the lowest degree of heterogeneity (I2 = 0%)	1328:1371	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	0	14	theme	midrib	110:115	arg1	silage					125:130	brown midrib sorghum silage	104:130	brown midrib sorghum silage	104:130	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.					
30415847	1	15	theme	dry	335:337	arg1	intake					346:351	dry matter intake	335:351	dry matter intake (DMI)	335:357	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	15	theme	dry	335:337	arg1	DMI					354:356	DMI	354:356	DMI	354:356	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	16	theme	feeding	189:195	arg1	silage					229:234	conventional sorghum silage	208:234	conventional sorghum silage (CSS)	208:240	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	16	theme	feeding	189:195	arg1	silage					263:268	conventional corn silage	245:268	conventional corn silage (CCS)	245:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	16	theme	feeding	189:195	arg1	cows					203:206	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	4	17	theme	publication	773:783	arg1	bias					785:788	publication bias	773:788	publication bias	773:788	The degree of heterogeneity was measured with the I2 statistic, and publication bias was determined with funnel plots and Egger's regression test.					
30415847	8	18	theme	silage	1258:1263	arg1	type					1265:1268	silage type	1258:1268	silage type	1258:1268	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	1	19	theme	cows	203:206	arg1	effects					178:184	the effects	174:184	the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	174:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	9	20	theme	BMRSS	1496:1500	arg1	diets					1502:1506	BMRSS diets	1496:1506	BMRSS diets	1496:1506	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	2	21	from	articles	421:428	arg1	Data					399:402	Data	399:402	Data from 9 published articles (1984 to 2015)	399:443	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	2	22	theme	BMRSS	561:565	arg1	diets					567:571	BMRSS diets	561:571	BMRSS diets	561:571	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	15	23	theme	silages	2758:2764	arg1	rates					2749:2753	the inclusion rates	2735:2753	the inclusion rates of silages in the diets	2735:2777	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	15	23	theme	silages	2758:2764	arg1	milk					2726:2729	milk	2726:2729	milk	2726:2729	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	3	24	theme	random	624:629	arg1	models					639:644	fixed or random effects models	615:644	fixed or random effects models with the Metafor package of R (https://www.R-project.org)	615:702	Statistical analysis was performed using fixed or random effects models with the Metafor package of R (https://www.R-project.org).					
30415847	3	25	dep	R	674:674	arg1	https					677:681	https	677:681	https://www.R-project.org	677:701	Statistical analysis was performed using fixed or random effects models with the Metafor package of R (https://www.R-project.org).					
30415847	4	26	theme	I2	755:756	arg1	statistic					758:766	the I2 statistic	751:766	the I2 statistic	751:766	The degree of heterogeneity was measured with the I2 statistic, and publication bias was determined with funnel plots and Egger's regression test.					
30415847	10	27	theme	milk	1800:1803	arg1	fat					1805:1807	milk fat	1800:1807	milk fat concentration (0.10%)	1800:1829	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	12	28	theme	fat	2225:2227	arg1	yields					2210:2215	yields	2210:2215	yields of milk fat and lactose	2210:2239	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	3	29	dep	https	677:681	arg1	//www.R-project.org					683:701	//www.R-project.org	683:701	https://www.R-project.org	677:701	Statistical analysis was performed using fixed or random effects models with the Metafor package of R (https://www.R-project.org).					
30415847	9	30	theme	milk	1673:1676	arg1	kg/d					1698:1701	0.16 kg/d	1693:1701	0.16 kg/d	1693:1701	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	30	theme	milk	1673:1676	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	30	theme	milk	1673:1676	arg1	yield					1686:1690	milk lactose yield	1673:1690	milk lactose yield (0.16 kg/d)	1673:1702	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	0	31	theme	conventional	53:64	arg1	sorghum					66:72	conventional sorghum	53:72	conventional sorghum	53:72	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.					
30415847	1	32	theme	midrib	296:301	arg1	silage					311:316	brown midrib sorghum silage	290:316	brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition	290:396	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	32	theme	midrib	296:301	arg1	BMRSS					319:323	BMRSS	319:323	BMRSS	319:323	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	2	33	theme	published	411:419	arg1	articles					421:428	9 published articles	409:428	9 published articles (1984 to 2015)	409:443	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	2	33	theme	published	411:419	arg1	2015					439:442	2015	439:442	2015	439:442	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	11	34	theme	CCS	2068:2070	arg1	diets					2072:2076	CCS diets	2068:2076	CCS diets	2068:2076	Meta-regression indicated that days in milk affected DMI and milk production when CSS diets were compared with BMRSS diets, and DMI when CCS diets were compared with BMRSS diets.					
30415847	11	35	theme	BMRSS	2042:2046	arg1	diets					2048:2052	BMRSS diets	2042:2052	BMRSS diets	2042:2052	Meta-regression indicated that days in milk affected DMI and milk production when CSS diets were compared with BMRSS diets, and DMI when CCS diets were compared with BMRSS diets.					
30415847	1	36	theme	silage	311:316	arg1	diets					326:330	brown midrib sorghum silage (BMRSS) diets	290:330	brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition	290:396	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	9	37	theme	decreased	1538:1546	arg1	yield					1650:1654	milk protein yield	1637:1654	milk protein yield (0.04 kg/d)	1637:1666	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	37	theme	decreased	1538:1546	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	37	theme	decreased	1538:1546	arg1	yield					1686:1690	milk lactose yield	1673:1690	milk lactose yield (0.16 kg/d)	1673:1702	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	37	theme	decreased	1538:1546	arg1	concentration					1586:1598	milk fat concentration	1577:1598	milk fat concentration (0.09%)	1577:1606	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	37	theme	decreased	1538:1546	arg1	yield					1618:1622	milk fat yield	1609:1622	milk fat yield (0.08 kg/d)	1609:1634	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	37	theme	decreased	1538:1546	arg1	kg/d					1570:1573	1.64 kg/d	1565:1573	1.64 kg/d	1565:1573	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	10	38	theme	milk	1846:1849	arg1	concentration					1859:1871	milk protein concentration	1846:1871	milk protein concentration (0.06%)	1846:1879	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	10	38	theme	milk	1846:1849	arg1	%					1878:1878	0.06%	1874:1878	0.06%	1874:1878	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	3	39	theme	R	674:674	arg1	package					663:669	the Metafor package	651:669	the Metafor package of R (https://www.R-project.org)	651:702	Statistical analysis was performed using fixed or random effects models with the Metafor package of R (https://www.R-project.org).					
30415847	3	40	with	models	639:644	arg1	package					663:669	the Metafor package	651:669	the Metafor package of R (https://www.R-project.org)	651:702	Statistical analysis was performed using fixed or random effects models with the Metafor package of R (https://www.R-project.org).					
30415847	8	41	dep	heterogeneity	1111:1123	arg1	=					1129:1129	=	1129:1129	=	1129:1129	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	15	42	theme	analysis	2640:2647	arg1	Results					2624:2630	Results	2624:2630	Results of this analysis	2624:2647	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	12	43	theme	dietary	2169:2175	arg1	fiber					2195:2199	the diet and dietary neutral detergent fiber	2156:2199	fiber	2195:2199	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	7	44	theme	publication	1034:1044	arg1	bias					1046:1049	publication bias	1034:1049	publication bias	1034:1049	No evidence of publication bias was observed for any variable tested.					
30415847	9	45	theme	milk	1577:1580	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	45	theme	milk	1577:1580	arg1	concentration					1586:1598	milk fat concentration	1577:1598	milk fat concentration (0.09%)	1577:1606	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	45	theme	milk	1577:1580	arg1	%					1605:1605	0.09%	1601:1605	0.09%	1601:1605	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	0	46	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.	0:131	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.					
30415847	1	47	theme	corn	258:261	arg1	CCS					271:273	CCS	271:273	CCS	271:273	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	47	theme	corn	258:261	arg1	silage					263:268	conventional corn silage	245:268	conventional corn silage (CCS)	245:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	47	theme	corn	258:261	arg1	cows					203:206	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	5	48	gly	heterogeneity	869:881	arg1	response					886:893	response	886:893	response	886:893	Other sources of heterogeneity of response were analyzed through meta-regression.					
30415847	8	49	theme	heterogeneity	1349:1361	arg1	degree					1339:1344	the lowest degree	1328:1344	the lowest degree of heterogeneity (I2 = 0%)	1328:1371	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	11	50	theme	milk	1992:1995	arg1	production					1997:2006	milk production	1992:2006	milk production	1992:2006	Meta-regression indicated that days in milk affected DMI and milk production when CSS diets were compared with BMRSS diets, and DMI when CCS diets were compared with BMRSS diets.					
30415847	12	51	from	rate	2138:2141	arg1	fiber					2195:2199	the diet and dietary neutral detergent fiber	2156:2199	fiber	2195:2199	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	12	51	from	rate	2138:2141	arg1	diet					2160:2163	the diet and dietary neutral detergent fiber	2156:2199	diet	2160:2163	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	12	52	theme	detergent	2185:2193	arg1	fiber					2195:2199	the diet and dietary neutral detergent fiber	2156:2199	fiber	2195:2199	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	11	53	theme	CSS	2013:2015	arg1	diets					2017:2021	CSS diets	2013:2021	CSS diets	2013:2021	Meta-regression indicated that days in milk affected DMI and milk production when CSS diets were compared with BMRSS diets, and DMI when CCS diets were compared with BMRSS diets.					
30415847	4	54	theme	regression	835:844	arg1	test					846:849	Egger's regression test	827:849	Egger's regression test	827:849	The degree of heterogeneity was measured with the I2 statistic, and publication bias was determined with funnel plots and Egger's regression test.					
30415847	8	55	theme	I2	1364:1365	arg1	%					1370:1370	I2 = 0%	1364:1370	I2 = 0%	1364:1370	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	8	55	theme	I2	1364:1365	arg1	heterogeneity					1349:1361	heterogeneity	1349:1361	heterogeneity (I2 = 0%)	1349:1371	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	2	56	dep	2015	439:442	arg1	to					436:437	to	436:437	to	436:437	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	12	57	theme	lactose	2233:2239	arg1	yields					2210:2215	yields	2210:2215	yields of milk fat and lactose	2210:2239	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	4	58	theme	funnel	810:815	arg1	plots					817:821	funnel plots	810:821	funnel plots	810:821	The degree of heterogeneity was measured with the I2 statistic, and publication bias was determined with funnel plots and Egger's regression test.					
30415847	0	59	theme	cows	44:47	arg1	Meta-analysis					21:33	Meta-analysis	21:33	Meta-analysis of dairy cows	21:47	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.					
30415847	5	60	theme	heterogeneity	869:881	arg1	sources					858:864	Other sources	852:864	Other sources of heterogeneity of response	852:893	Other sources of heterogeneity of response were analyzed through meta-regression.					
30415847	8	61	theme	intake	1238:1243	arg1	responses					1245:1253	intake responses	1238:1253	intake responses to silage type	1238:1268	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	8	62	theme	variable	1421:1428	arg1	responses					1403:1411	the responses	1399:1411	the responses of this variable to silage type	1399:1443	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	8	62	theme	variable	1421:1428	arg1	consistent					1455:1464	consistent	1455:1464	consistent	1455:1464	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	1	63	theme	conventional	208:219	arg1	silage					229:234	conventional sorghum silage	208:234	conventional sorghum silage (CSS)	208:240	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	63	theme	conventional	208:219	arg1	CSS					237:239	CSS	237:239	CSS	237:239	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	63	theme	conventional	208:219	arg1	cows					203:206	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	8	64	theme	silage	1433:1438	arg1	type					1440:1443	silage type	1433:1443	silage type	1433:1443	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	9	65	theme	milk	1609:1612	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	65	theme	milk	1609:1612	arg1	yield					1618:1622	milk fat yield	1609:1622	milk fat yield (0.08 kg/d)	1609:1634	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	65	theme	milk	1609:1612	arg1	kg/d					1630:1633	0.08 kg/d	1625:1633	0.08 kg/d	1625:1633	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	2	66	with	diets	467:471	arg1	comparisons					491:501	7 means comparisons	483:501	7 means comparisons	483:501	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	2	66	with	diets	467:471	arg1	CCS					517:519	CCS	517:519	CCS (13 means comparisons; 204 cows)	517:552	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	2	66	with	diets	467:471	arg1	CSS					478:480	CSS	478:480	CSS (7 means comparisons; 104 cows)	478:512	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	2	66	with	diets	467:471	arg1	comparisons					531:541	13 means comparisons	522:541	13 means comparisons	522:541	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	15	67	from	effects	2707:2713	arg1	rates					2749:2753	the inclusion rates	2735:2753	the inclusion rates of silages in the diets	2735:2777	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	15	67	from	effects	2707:2713	arg1	milk					2726:2729	milk	2726:2729	milk	2726:2729	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	6	68	theme	effect	944:949	arg1	size					951:954	Estimated effect size	934:954	Estimated effect size	934:954	Estimated effect size was calculated for DMI, milk production, and milk composition.					
30415847	14	69	theme	small	2483:2487	arg1	size					2496:2499	the small sample size	2479:2499	the small sample size	2479:2499	However, the small sample size may have influenced these results by increasing the margin of the error and, concurrently, the power of the meta-analysis.					
30415847	15	70	theme	inclusion	2739:2747	arg1	rates					2749:2753	the inclusion rates	2735:2753	the inclusion rates of silages in the diets	2735:2777	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	4	71	theme	heterogeneity	719:731	arg1	degree					709:714	The degree	705:714	The degree of heterogeneity	705:731	The degree of heterogeneity was measured with the I2 statistic, and publication bias was determined with funnel plots and Egger's regression test.					
30415847	1	72	theme	milk	381:384	arg1	composition					386:396	milk composition	381:396	milk composition	381:396	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	15	73	from	milk	2726:2729	arg1	diets					2773:2777	the diets	2769:2777	the diets	2769:2777	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	3	74	theme	Statistical	574:584	arg1	analysis					586:593	Statistical analysis	574:593	Statistical analysis	574:593	Statistical analysis was performed using fixed or random effects models with the Metafor package of R (https://www.R-project.org).					
30415847	14	75	theme	meta-analysis	2609:2621	arg1	power					2596:2600	the power	2592:2600	the power of the meta-analysis	2592:2621	However, the small sample size may have influenced these results by increasing the margin of the error and, concurrently, the power of the meta-analysis.					
30415847	9	76	theme	milk	1637:1640	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	76	theme	milk	1637:1640	arg1	yield					1650:1654	milk protein yield	1637:1654	milk protein yield (0.04 kg/d)	1637:1666	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	76	theme	milk	1637:1640	arg1	kg/d					1662:1665	0.04 kg/d	1657:1665	0.04 kg/d	1657:1665	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	0	77	theme	sorghum	117:123	arg1	silage					125:130	brown midrib sorghum silage	104:130	brown midrib sorghum silage	104:130	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.					
30415847	10	78	theme	protein	1851:1857	arg1	concentration					1859:1871	milk protein concentration	1846:1871	milk protein concentration (0.06%)	1846:1879	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	10	78	theme	protein	1851:1857	arg1	%					1878:1878	0.06%	1874:1878	0.06%	1874:1878	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	15	79	from	rates	2749:2753	arg1	diets					2773:2777	the diets	2769:2777	the diets	2769:2777	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	10	80	theme	BMRSS	1778:1782	arg1	diets					1784:1788	BMRSS diets	1778:1788	BMRSS diets	1778:1788	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	1	81	theme	matter	339:344	arg1	intake					346:351	dry matter intake	335:351	dry matter intake (DMI)	335:357	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	81	theme	matter	339:344	arg1	DMI					354:356	DMI	354:356	DMI	354:356	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	82	theme	dairy	197:201	arg1	silage					229:234	conventional sorghum silage	208:234	conventional sorghum silage (CSS)	208:240	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	82	theme	dairy	197:201	arg1	silage					263:268	conventional corn silage	245:268	conventional corn silage (CCS)	245:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	82	theme	dairy	197:201	arg1	cows					203:206	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	11	83	theme	BMRSS	2097:2101	arg1	diets					2103:2107	BMRSS diets	2097:2107	BMRSS diets	2097:2107	Meta-regression indicated that days in milk affected DMI and milk production when CSS diets were compared with BMRSS diets, and DMI when CCS diets were compared with BMRSS diets.					
30415847	12	84	theme	milk	2220:2223	arg1	fat					2225:2227	milk fat	2220:2227	milk fat	2220:2227	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	8	85	theme	heterogeneity	1111:1123	arg1	degree					1101:1106	The highest degree	1089:1106	The highest degree of heterogeneity (I2 = 41.5 and 72.6%)	1089:1145	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	14	86	theme	error	2567:2571	arg1	margin					2553:2558	the margin	2549:2558	the margin of the error	2549:2571	However, the small sample size may have influenced these results by increasing the margin of the error and, concurrently, the power of the meta-analysis.					
30415847	2	87	dep	comparisons	491:501	arg1	cows					508:511	104 cows	504:511	104 cows	504:511	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	12	88	theme	CCS	2261:2263	arg1	diets					2275:2279	CCS and BMRSS diets	2261:2279	CCS and BMRSS diets	2261:2279	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	10	89	theme	fat	1805:1807	arg1	concentration					1809:1821	milk fat concentration	1800:1821	milk fat concentration (0.10%)	1800:1829	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	10	89	theme	fat	1805:1807	arg1	%					1828:1828	0.10%	1824:1828	0.10%	1824:1828	Compared with CCS diets, cows fed BMRSS diets increased milk fat concentration (0.10%), but decreased milk protein concentration (0.06%) and tended to increase lactose yield (0.08 kg/d).					
30415847	3	90	theme	effects	631:637	arg1	models					639:644	fixed or random effects models	615:644	fixed or random effects models with the Metafor package of R (https://www.R-project.org)	615:702	Statistical analysis was performed using fixed or random effects models with the Metafor package of R (https://www.R-project.org).					
30415847	5	91	theme	response	886:893	arg1	heterogeneity					869:881	heterogeneity	869:881	heterogeneity of response	869:893	Other sources of heterogeneity of response were analyzed through meta-regression.					
30415847	2	92	dep	comparisons	531:541	arg1	cows					548:551	204 cows	544:551	204 cows	544:551	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	9	93	theme	protein	1642:1648	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	93	theme	protein	1642:1648	arg1	yield					1650:1654	milk protein yield	1637:1654	milk protein yield (0.04 kg/d)	1637:1666	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	93	theme	protein	1642:1648	arg1	kg/d					1662:1665	0.04 kg/d	1657:1665	0.04 kg/d	1657:1665	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	1	94	theme	milk	360:363	arg1	production					365:374	milk production	360:374	milk production	360:374	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	15	95	theme	additional	2662:2671	arg1	research					2673:2680	additional research	2662:2680	additional research	2662:2680	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	9	96	theme	CSS	1518:1520	arg1	diets					1522:1526	CSS diets	1518:1526	CSS diets	1518:1526	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	8	97	theme	milk	1308:1311	arg1	production					1313:1322	milk production	1308:1322	milk production	1308:1322	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	9	98	theme	lactose	1678:1684	arg1	kg/d					1698:1701	0.16 kg/d	1693:1701	0.16 kg/d	1693:1701	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	98	theme	lactose	1678:1684	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	98	theme	lactose	1678:1684	arg1	yield					1686:1690	milk lactose yield	1673:1690	milk lactose yield (0.16 kg/d)	1673:1702	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	3	99	theme	Metafor	655:661	arg1	package					663:669	the Metafor package	651:669	the Metafor package of R (https://www.R-project.org)	651:702	Statistical analysis was performed using fixed or random effects models with the Metafor package of R (https://www.R-project.org).					
30415847	0	100	theme	corn	77:80	arg1	silages					82:88	corn silages	77:88	corn silages	77:88	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.					
30415847	1	101	theme	sorghum	303:309	arg1	silage					311:316	brown midrib sorghum silage	290:316	brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition	290:396	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	101	theme	sorghum	303:309	arg1	BMRSS					319:323	BMRSS	319:323	BMRSS	319:323	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	2	102	theme	means	525:529	arg1	CCS					517:519	CCS	517:519	CCS (13 means comparisons; 204 cows)	517:552	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	2	102	theme	means	525:529	arg1	comparisons					531:541	13 means comparisons	522:541	13 means comparisons	522:541	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	2	103	used	used	450:453	arg2	Data					399:402	Data	399:402	Data from 9 published articles (1984 to 2015)	399:443	Data from 9 published articles (1984 to 2015) were used to contrast diets with CSS (7 means comparisons; 104 cows) or CCS (13 means comparisons; 204 cows) versus BMRSS diets.					
30415847	7	104	theme	bias	1046:1049	arg1	evidence					1022:1029	No evidence	1019:1029	No evidence of publication bias	1019:1049	No evidence of publication bias was observed for any variable tested.					
30415847	9	105	theme	milk	1548:1551	arg1	yield					1650:1654	milk protein yield	1637:1654	milk protein yield (0.04 kg/d)	1637:1666	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	105	theme	milk	1548:1551	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	105	theme	milk	1548:1551	arg1	yield					1686:1690	milk lactose yield	1673:1690	milk lactose yield (0.16 kg/d)	1673:1702	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	105	theme	milk	1548:1551	arg1	concentration					1586:1598	milk fat concentration	1577:1598	milk fat concentration (0.09%)	1577:1606	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	105	theme	milk	1548:1551	arg1	yield					1618:1622	milk fat yield	1609:1622	milk fat yield (0.08 kg/d)	1609:1634	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	105	theme	milk	1548:1551	arg1	kg/d					1570:1573	1.64 kg/d	1565:1573	1.64 kg/d	1565:1573	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	8	106	theme	lowest	1332:1337	arg1	degree					1339:1344	the lowest degree	1328:1344	the lowest degree of heterogeneity (I2 = 0%)	1328:1371	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	12	107	theme	neutral	2177:2183	arg1	fiber					2195:2199	the diet and dietary neutral detergent fiber	2156:2199	fiber	2195:2199	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	0	108	theme	brown	104:108	arg1	silage					125:130	brown midrib sorghum silage	104:130	brown midrib sorghum silage	104:130	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.					
30415847	6	109	theme	Estimated	934:942	arg1	size					951:954	Estimated effect size	934:954	Estimated effect size	934:954	Estimated effect size was calculated for DMI, milk production, and milk composition.					
30415847	12	110	theme	silage	2146:2151	arg1	rate					2138:2141	the inclusion rate	2124:2141	the inclusion rate of silage in the diet and dietary neutral detergent fiber	2124:2199	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	1	111	from	diets	326:330	arg1	intake					346:351	dry matter intake	335:351	dry matter intake (DMI)	335:357	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	111	from	diets	326:330	arg1	production					365:374	milk production	360:374	milk production	360:374	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	111	from	diets	326:330	arg1	DMI					354:356	DMI	354:356	DMI	354:356	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	111	from	diets	326:330	arg1	composition					386:396	milk composition	381:396	milk composition	381:396	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	12	112	theme	BMRSS	2269:2273	arg1	diets					2275:2279	CCS and BMRSS diets	2261:2279	CCS and BMRSS diets	2261:2279	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
30415847	8	113	theme	dependent	1174:1182	arg1	variables					1184:1192	dependent variables	1174:1192	dependent variables tested in both comparisons	1174:1219	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	8	114	theme	=	1367:1367	arg1	%					1370:1370	I2 = 0%	1364:1370	I2 = 0%	1364:1370	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	8	114	theme	=	1367:1367	arg1	heterogeneity					1349:1361	heterogeneity	1349:1361	heterogeneity (I2 = 0%)	1349:1371	The highest degree of heterogeneity (I2 = 41.5 and 72.6%) was observed for DMI among dependent variables tested in both comparisons, indicating that intake responses to silage type are rather inconsistent; in contrast, milk production had the lowest degree of heterogeneity (I2 = 0%), supporting the idea that the responses of this variable to silage type were very consistent across studies.					
30415847	0	115	theme	dairy	38:42	arg1	cows					44:47	dairy cows	38:47	dairy cows	38:47	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.					
30415847	5	116	theme	Other	852:856	arg1	sources					858:864	Other sources	852:864	Other sources of heterogeneity of response	852:893	Other sources of heterogeneity of response were analyzed through meta-regression.					
30415847	9	117	theme	fat	1582:1584	arg1	production					1553:1562	decreased milk production	1538:1562	decreased milk production (1.64 kg/d)	1538:1574	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	117	theme	fat	1582:1584	arg1	concentration					1586:1598	milk fat concentration	1577:1598	milk fat concentration (0.09%)	1577:1606	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	9	117	theme	fat	1582:1584	arg1	%					1605:1605	0.09%	1601:1605	0.09%	1601:1605	Compared with BMRSS diets, cows fed CSS diets exhibited decreased milk production (1.64 kg/d), milk fat concentration (0.09%), milk fat yield (0.08 kg/d), milk protein yield (0.04 kg/d), and milk lactose yield (0.16 kg/d) and tended to decrease DMI (0.83 kg/d).					
30415847	15	118	theme	days	2718:2721	arg1	effects					2707:2713	the effects	2703:2713	the effects of days in milk and the inclusion rates of silages in the diets	2703:2777	Results of this analysis suggest that additional research is needed to explore the effects of days in milk and the inclusion rates of silages in the diets when comparing BMRSS with CSS or CCS.					
30415847	6	119	theme	milk	980:983	arg1	production					985:994	milk production	980:994	milk production	980:994	Estimated effect size was calculated for DMI, milk production, and milk composition.					
30415847	1	120	theme	brown	290:294	arg1	silage					311:316	brown midrib sorghum silage	290:316	brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition	290:396	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	120	theme	brown	290:294	arg1	BMRSS					319:323	BMRSS	319:323	BMRSS	319:323	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	121	dep	cows	203:206	arg1	silage					229:234	conventional sorghum silage	208:234	conventional sorghum silage (CSS)	208:240	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	121	dep	cows	203:206	arg1	CCS					271:273	CCS	271:273	CCS	271:273	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	121	dep	cows	203:206	arg1	silage					263:268	conventional corn silage	245:268	conventional corn silage (CCS)	245:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	121	dep	cows	203:206	arg1	CSS					237:239	CSS	237:239	CSS	237:239	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	121	dep	cows	203:206	arg1	cows					203:206	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	13	122	theme	lactation	2305:2313	arg1	performance					2315:2325	lactation performance	2305:2325	lactation performance	2305:2325	Overall, lactation performance improved when cows were fed diets formulated with BMRSS compared with CSS, but performance was not different for cows fed BMRSS and CCS diets.					
30415847	1	123	theme	sorghum	221:227	arg1	silage					229:234	conventional sorghum silage	208:234	conventional sorghum silage (CSS)	208:240	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	123	theme	sorghum	221:227	arg1	CSS					237:239	CSS	237:239	CSS	237:239	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	1	123	theme	sorghum	221:227	arg1	cows					203:206	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS)	189:274	A meta-analysis was conducted to compare the effects of feeding dairy cows conventional sorghum silage (CSS) or conventional corn silage (CCS) compared with brown midrib sorghum silage (BMRSS) diets on dry matter intake (DMI), milk production, and milk composition.					
30415847	0	124	dep	communication	6:18	arg1	fed					49:51	fed	49:51	fed conventional sorghum or corn silages compared with brown midrib sorghum silage	49:130	Short communication: Meta-analysis of dairy cows fed conventional sorghum or corn silages compared with brown midrib sorghum silage.					
30415847	12	125	theme	inclusion	2128:2136	arg1	rate					2138:2141	the inclusion rate	2124:2141	the inclusion rate of silage in the diet and dietary neutral detergent fiber	2124:2199	Additionally, the inclusion rate of silage in the diet and dietary neutral detergent fiber affected yields of milk fat and lactose, respectively, when CCS and BMRSS diets were compared.					
31743704	6	0	dep	transparency	1367:1378	arg1	the					1363:1365	the	1363:1365	the	1363:1365	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	6	1	theme	water	1314:1318	arg1	barrier					1326:1332	water vapor barrier	1314:1332	water vapor barrier	1314:1332	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	1	2	theme	growing	166:172	arg1	demand					174:179	the growing demand	162:179	the growing demand for high quality food	162:201	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	4	3	theme	microscope	914:923	arg1	Characterization					856:871	Characterization	856:871	Characterization of macromorphology and scanning electron microscope	856:923	Characterization of macromorphology and scanning electron microscope indicated the starch composite films with WMSNs/KC were smooth, uniform, and transparent.					
31743704	6	4	theme	starch-based	1189:1200	arg1	films					1202:1206	the pure starch-based films	1180:1206	the pure starch-based films	1180:1206	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	1	5	theme	packing	251:257	arg1	material					259:266	packing material	251:266	packing material with optimized physical, mechanical properties and antimicrobial activity	251:340	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	4	6	theme	starch	939:944	arg1	smooth					981:986	smooth	981:986	smooth	981:986	Characterization of macromorphology and scanning electron microscope indicated the starch composite films with WMSNs/KC were smooth, uniform, and transparent.					
31743704	4	6	theme	starch	939:944	arg1	films					956:960	the starch composite films	935:960	the starch composite films with WMSNs/KC	935:974	Characterization of macromorphology and scanning electron microscope indicated the starch composite films with WMSNs/KC were smooth, uniform, and transparent.					
31743704	7	7	theme	starch	1438:1443	arg1	films					1455:1459	the starch composite films	1434:1459	the starch composite films	1434:1459	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	5	8	theme	X-ray	1015:1019	arg1	pattern					1033:1039	X-ray diffraction pattern and Thermogravimetric analysis	1015:1070	pattern	1033:1039	X-ray diffraction pattern and Thermogravimetric analysis also showed strong interactions such as hydrogen bond formation among films, WMSNs/KC and SA.					
31743704	7	9	from	microorganisms	1529:1542	arg1	foods					1547:1551	foods	1547:1551	foods	1547:1551	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	3	10	theme	%	853:853	arg1	concentration					833:845	a concentration	831:845	a concentration of 0-9%	831:853	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
31743704	0	11	theme	salicylic	107:115	arg1	acid					117:120	salicylic acid	107:120	salicylic acid	107:120	Mechanical properties and antibacterial activities of novel starch-based composite films incorporated with salicylic acid.					
31743704	6	12	theme	thermal	1338:1344	arg1	stability					1346:1354	thermal stability	1338:1354	thermal stability	1338:1354	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	5	13	theme	diffraction	1021:1031	arg1	pattern					1033:1039	X-ray diffraction pattern and Thermogravimetric analysis	1015:1070	pattern	1033:1039	X-ray diffraction pattern and Thermogravimetric analysis also showed strong interactions such as hydrogen bond formation among films, WMSNs/KC and SA.					
31743704	3	14	used	used	727:730	arg2	stabilizer					752:761	stabilizer	752:761	stabilizer	752:761	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
31743704	3	14	used	used	727:730	arg2	WMSNs					581:585	WMSNs	581:585	WMSNs	581:585	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
31743704	3	14	used	used	727:730	arg2	nanofiller					737:746	nanofiller	737:746	nanofiller	737:746	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
31743704	2	15	theme	salicylic	417:425	arg1	SA					433:434	SA	433:434	SA	433:434	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	2	15	theme	salicylic	417:425	arg1	acid					427:430	salicylic acid	417:430	salicylic acid (SA)	417:435	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	2	16	theme	antimicrobial	519:531	arg1	activity					533:540	antimicrobial activity	519:540	antimicrobial activity	519:540	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	4	17	theme	macromorphology	876:890	arg1	Characterization					856:871	Characterization	856:871	Characterization of macromorphology and scanning electron microscope	856:923	Characterization of macromorphology and scanning electron microscope indicated the starch composite films with WMSNs/KC were smooth, uniform, and transparent.					
31743704	2	18	theme	maize	446:450	arg1	WMSNs/KC					488:495	WMSNs/KC	488:495	WMSNs/KC	488:495	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	2	18	theme	maize	446:450	arg1	nanoparticles/κ-carrageenan					459:485	waxy maize starch nanoparticles/κ-carrageenan	441:485	waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC)	441:496	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	1	19	theme	high	185:188	arg1	food					198:201	high quality food	185:201	high quality food	185:201	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	2	20	theme	starch	452:457	arg1	WMSNs/KC					488:495	WMSNs/KC	488:495	WMSNs/KC	488:495	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	2	20	theme	starch	452:457	arg1	nanoparticles/κ-carrageenan					459:485	waxy maize starch nanoparticles/κ-carrageenan	441:485	waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC)	441:496	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	4	21	theme	electron	905:912	arg1	microscope					914:923	scanning electron microscope	896:923	scanning electron microscope	896:923	Characterization of macromorphology and scanning electron microscope indicated the starch composite films with WMSNs/KC were smooth, uniform, and transparent.					
31743704	6	22	from	break	1398:1402	arg1	elongation					1384:1393	elongation	1384:1393	elongation	1384:1393	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	6	22	from	break	1398:1402	arg1	transparency					1367:1378	transparency	1367:1378	transparency	1367:1378	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	1	23	theme	quality	190:196	arg1	food					198:201	high quality food	185:201	high quality food	185:201	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	0	24	theme	Mechanical	0:9	arg1	properties					11:20	Mechanical properties	0:20	Mechanical properties	0:20	Mechanical properties and antibacterial activities of novel starch-based composite films incorporated with salicylic acid.					
31743704	1	25	theme	optimized	273:281	arg1	properties					304:313	optimized physical, mechanical properties	273:313	properties	304:313	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	4	26	theme	scanning	896:903	arg1	microscope					914:923	scanning electron microscope	896:923	scanning electron microscope	896:923	Characterization of macromorphology and scanning electron microscope indicated the starch composite films with WMSNs/KC were smooth, uniform, and transparent.					
31743704	6	27	theme	composite	1213:1221	arg1	films					1223:1227	the composite films	1209:1227	the composite films reinforced by the addition of WMSNs/KC	1209:1266	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	0	28	theme	antibacterial	26:38	arg1	activities					40:49	antibacterial activities	26:49	antibacterial activities	26:49	Mechanical properties and antibacterial activities of novel starch-based composite films incorporated with salicylic acid.					
31743704	1	29	theme	physical	283:290	arg1	properties					304:313	optimized physical, mechanical properties	273:313	properties	304:313	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	7	30	theme	composite	1445:1453	arg1	films					1455:1459	the starch composite films	1434:1459	the starch composite films	1434:1459	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	7	31	theme	excellent	1468:1476	arg1	activity					1492:1499	excellent antimicrobial activity	1468:1499	excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis	1468:1615	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	5	32	theme	strong	1084:1089	arg1	formation					1126:1134	hydrogen bond formation	1112:1134	hydrogen bond formation	1112:1134	X-ray diffraction pattern and Thermogravimetric analysis also showed strong interactions such as hydrogen bond formation among films, WMSNs/KC and SA.					
31743704	5	32	theme	strong	1084:1089	arg1	interactions					1091:1102	strong interactions	1084:1102	strong interactions such as hydrogen bond formation among films, WMSNs/KC and SA	1084:1163	X-ray diffraction pattern and Thermogravimetric analysis also showed strong interactions such as hydrogen bond formation among films, WMSNs/KC and SA.					
31743704	7	33	theme	antimicrobial	1478:1490	arg1	activity					1492:1499	excellent antimicrobial activity	1468:1499	excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis	1468:1615	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	4	34	with	films	956:960	arg1	WMSNs/KC					967:974	WMSNs/KC	967:974	WMSNs/KC	967:974	Characterization of macromorphology and scanning electron microscope indicated the starch composite films with WMSNs/KC were smooth, uniform, and transparent.					
31743704	1	35	theme	mechanical	293:302	arg1	properties					304:313	optimized physical, mechanical properties	273:313	properties	304:313	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	2	36	theme	mechanical	558:567	arg1	properties					569:578	the mechanical properties	554:578	the mechanical properties	554:578	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	3	37	theme	recrystallisation	627:643	arg1	method					645:650	recrystallisation method	627:650	recrystallisation method	627:650	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
31743704	1	38	theme	novel	206:210	arg1	film					243:246	a novel and excellent starch composite film	204:246	a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity	204:340	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	3	39	dep	nanofiller	737:746	arg1	a					735:735	a	735:735	a	735:735	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
31743704	3	40	theme	tapioca	801:807	arg1	films					822:826	hydroxypropyl tapioca starch-based films	787:826	hydroxypropyl tapioca starch-based films	787:826	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
31743704	2	41	theme	waxy	441:444	arg1	WMSNs/KC					488:495	WMSNs/KC	488:495	WMSNs/KC	488:495	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	2	41	theme	waxy	441:444	arg1	nanoparticles/κ-carrageenan					459:485	waxy maize starch nanoparticles/κ-carrageenan	441:485	waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC)	441:496	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	1	42	with	material	259:266	arg1	properties					304:313	optimized physical, mechanical properties	273:313	properties	304:313	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	1	42	with	material	259:266	arg1	activity					333:340	antimicrobial activity	319:340	antimicrobial activity	319:340	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	0	43	theme	starch-based	60:71	arg1	films					83:87	novel starch-based composite films	54:87	novel starch-based composite films	54:87	Mechanical properties and antibacterial activities of novel starch-based composite films incorporated with salicylic acid.					
31743704	3	44	theme	hydroxypropyl	787:799	arg1	films					822:826	hydroxypropyl tapioca starch-based films	787:826	hydroxypropyl tapioca starch-based films	787:826	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
31743704	6	45	theme	tensile	1296:1302	arg1	strength					1304:1311	the tensile strength	1292:1311	the tensile strength	1292:1311	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	0	46	theme	novel	54:58	arg1	films					83:87	novel starch-based composite films	54:87	novel starch-based composite films	54:87	Mechanical properties and antibacterial activities of novel starch-based composite films incorporated with salicylic acid.					
31743704	1	47	theme	antimicrobial	319:331	arg1	activity					333:340	antimicrobial activity	319:340	antimicrobial activity	319:340	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	4	48	theme	composite	946:954	arg1	smooth					981:986	smooth	981:986	smooth	981:986	Characterization of macromorphology and scanning electron microscope indicated the starch composite films with WMSNs/KC were smooth, uniform, and transparent.					
31743704	4	48	theme	composite	946:954	arg1	films					956:960	the starch composite films	935:960	the starch composite films with WMSNs/KC	935:974	Characterization of macromorphology and scanning electron microscope indicated the starch composite films with WMSNs/KC were smooth, uniform, and transparent.					
31743704	5	49	theme	hydrogen	1112:1119	arg1	formation					1126:1134	hydrogen bond formation	1112:1134	hydrogen bond formation	1112:1134	X-ray diffraction pattern and Thermogravimetric analysis also showed strong interactions such as hydrogen bond formation among films, WMSNs/KC and SA.					
31743704	5	50	theme	bond	1121:1124	arg1	formation					1126:1134	hydrogen bond formation	1112:1134	hydrogen bond formation	1112:1134	X-ray diffraction pattern and Thermogravimetric analysis also showed strong interactions such as hydrogen bond formation among films, WMSNs/KC and SA.					
31743704	1	51	theme	food	134:137	arg1	contamination					139:151	food contamination	134:151	food contamination	134:151	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	3	52	theme	starch-based	809:820	arg1	films					822:826	hydroxypropyl tapioca starch-based films	787:826	hydroxypropyl tapioca starch-based films	787:826	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
31743704	2	53	used	used	503:506	arg2	films					393:397	Starch-based composite films	370:397	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC)	370:496	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	5	54	theme	Thermogravimetric	1045:1061	arg1	analysis					1063:1070	X-ray diffraction pattern and Thermogravimetric analysis	1015:1070	analysis	1063:1070	X-ray diffraction pattern and Thermogravimetric analysis also showed strong interactions such as hydrogen bond formation among films, WMSNs/KC and SA.					
31743704	6	55	theme	vapor	1320:1324	arg1	barrier					1326:1332	water vapor barrier	1314:1332	water vapor barrier	1314:1332	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	6	56	theme	pure	1184:1187	arg1	films					1202:1206	the pure starch-based films	1180:1206	the pure starch-based films	1180:1206	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	1	57	theme	excellent	216:224	arg1	film					243:246	a novel and excellent starch composite film	204:246	a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity	204:340	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	7	58	theme	typical	1511:1517	arg1	subtilis					1608:1615	Bacillus subtilis	1599:1615	Bacillus subtilis	1599:1615	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	7	58	theme	typical	1511:1517	arg1	aureus					1587:1592	Staphylococcus aureus	1572:1592	Staphylococcus aureus	1572:1592	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	7	58	theme	typical	1511:1517	arg1	coli					1566:1569	Escherichia coli	1554:1569	Escherichia coli	1554:1569	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	7	58	theme	typical	1511:1517	arg1	microorganisms					1529:1542	three typical undesired microorganisms	1505:1542	three typical undesired microorganisms in foods	1505:1551	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	7	59	theme	undesired	1519:1527	arg1	subtilis					1608:1615	Bacillus subtilis	1599:1615	Bacillus subtilis	1599:1615	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	7	59	theme	undesired	1519:1527	arg1	aureus					1587:1592	Staphylococcus aureus	1572:1592	Staphylococcus aureus	1572:1592	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	7	59	theme	undesired	1519:1527	arg1	coli					1566:1569	Escherichia coli	1554:1569	Escherichia coli	1554:1569	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	7	59	theme	undesired	1519:1527	arg1	microorganisms					1529:1542	three typical undesired microorganisms	1505:1542	three typical undesired microorganisms in foods	1505:1551	Moreover, the starch composite films showed excellent antimicrobial activity for three typical undesired microorganisms in foods, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis.					
31743704	1	60	theme	starch	226:231	arg1	film					243:246	a novel and excellent starch composite film	204:246	a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity	204:340	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	0	61	theme	films	83:87	arg1	properties					11:20	Mechanical properties	0:20	Mechanical properties	0:20	Mechanical properties and antibacterial activities of novel starch-based composite films incorporated with salicylic acid.					
31743704	0	61	theme	films	83:87	arg1	activities					40:49	antibacterial activities	26:49	antibacterial activities	26:49	Mechanical properties and antibacterial activities of novel starch-based composite films incorporated with salicylic acid.					
31743704	6	62	theme	WMSNs/KC	1259:1266	arg1	addition					1247:1254	the addition	1243:1254	the addition of WMSNs/KC	1243:1266	Compared with the pure starch-based films, the composite films reinforced by the addition of WMSNs/KC significantly increased the tensile strength, water vapor barrier and thermal stability, while the transparency and elongation at break decreased slightly.					
31743704	2	63	theme	composite	383:391	arg1	films					393:397	Starch-based composite films	370:397	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC)	370:496	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	1	64	theme	composite	233:241	arg1	film					243:246	a novel and excellent starch composite film	204:246	a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity	204:340	To control food contamination and meet the growing demand for high quality food, a novel and excellent starch composite film as packing material with optimized physical, mechanical properties and antimicrobial activity was produced in this paper.					
31743704	0	65	theme	composite	73:81	arg1	films					83:87	novel starch-based composite films	54:87	novel starch-based composite films	54:87	Mechanical properties and antibacterial activities of novel starch-based composite films incorporated with salicylic acid.					
31743704	2	66	theme	Starch-based	370:381	arg1	films					393:397	Starch-based composite films	370:397	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC)	370:496	Starch-based composite films incorporated with salicylic acid (SA) and waxy maize starch nanoparticles/κ-carrageenan (WMSNs/KC) were used to achieve antimicrobial activity and improve the mechanical properties.					
31743704	3	67	dep	form	691:694	arg1	WMSNs/KC					696:703	WMSNs/KC	696:703	to form WMSNs/KC by self-assembly	688:720	WMSNs were fabricated through enzymolysis and recrystallisation method, followed by individually adding KC to form WMSNs/KC by self-assembly, and used as a nanofiller and stabilizer to be incorporated into hydroxypropyl tapioca starch-based films at a concentration of 0-9%.					
29413003	2	0	theme	microbial	466:474	arg1	growth					476:481	microbial growth	466:481	microbial growth	466:481	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	3	1	from	microbiota	711:720	arg1	samples					735:741	different samples	725:741	different samples	725:741	Meanwhile, variability in the predominant microbiota in different samples was investigated by culture-dependent and -independent methods.					
29413003	2	2	theme	1	352:352	arg1	%					353:353	%	353:353	%	353:353	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	4	3	theme	COS	910:912	arg1	samples					922:928	COS treated samples	910:928	COS treated samples	910:928	Based on sensory analysis, shelf-life of silver carp fillets was 4 days for the control and 6 days for COS treated samples.					
29413003	2	4	theme	basic	529:533	arg1	putrescine					545:554	putrescine	545:554	putrescine	545:554	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	4	theme	basic	529:533	arg1	nitrogen					535:542	total volatile basic nitrogen	514:542	total volatile basic nitrogen	514:542	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	4	theme	basic	529:533	arg1	hypoxanthine					572:583	hypoxanthine	572:583	hypoxanthine	572:583	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	4	theme	basic	529:533	arg1	cadaverine					557:566	cadaverine	557:566	cadaverine	557:566	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	9	5	theme	fillets	1474:1480	arg1	life					1454:1457	the shelf life	1444:1457	the shelf life of silver carp fillets by 2 days	1444:1490	Therefore, COS improved the quality of fillets and prolonged the shelf life of silver carp fillets by 2 days during chilled storage, which was mainly due to their modulating effects on microbiota.					
29413003	2	6	theme	ribonucleotide	653:666	arg1	degradation					599:609	degradation	599:609	degradation of inosine monophosphate and hypoxanthine ribonucleotide	599:666	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	9	7	theme	chilled	1499:1505	arg1	storage					1507:1513	chilled storage	1499:1513	chilled storage	1499:1513	Therefore, COS improved the quality of fillets and prolonged the shelf life of silver carp fillets by 2 days during chilled storage, which was mainly due to their modulating effects on microbiota.					
29413003	0	8	from	Effects	0:6	arg1	composition					51:61	microbiota composition	40:61	microbiota composition of silver carp (Hypophthalmichthys molitrix)	40:106	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	2	9	theme	hypoxanthine	640:651	arg1	ribonucleotide					653:666	hypoxanthine ribonucleotide	640:666	hypoxanthine ribonucleotide	640:666	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	6	10	theme	Shewanella	1163:1172	arg1	growth					1125:1130	the growth	1121:1130	the growth of Pseudomonas, Aeromonas, and Shewanella	1121:1172	However, COS inhibited the growth of Pseudomonas, Aeromonas, and Shewanella significantly.					
29413003	5	11	dep	Meanwhile	931:939	arg1	Pseudomonas					942:952	Meanwhile, Pseudomonas, followed by Aeromonas, Acinetobacter, and Shewanella	931:1006	Pseudomonas	942:952	Meanwhile, Pseudomonas, followed by Aeromonas, Acinetobacter, and Shewanella were dominated in the control samples at day 4 and contributed to fish spoilage at day 6.					
29413003	8	12	theme	COS	1340:1342	arg1	samples					1352:1358	COS treated samples	1340:1358	COS treated samples	1340:1358	With the growth of Pseudomonas, COS treated samples were spoiled at day 8.					
29413003	8	13	theme	Pseudomonas	1327:1337	arg1	growth					1317:1322	the growth	1313:1322	the growth of Pseudomonas	1313:1337	With the growth of Pseudomonas, COS treated samples were spoiled at day 8.					
29413003	1	14	theme	quality	274:280	arg1	fillets					312:318	quality and microbiota of silver carp fillets	274:318	quality and microbiota of silver carp fillets stored at 4 °C	274:333	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	3	15	from	variability	680:690	arg1	microbiota					711:720	the predominant microbiota	695:720	the predominant microbiota in different samples	695:741	Meanwhile, variability in the predominant microbiota in different samples was investigated by culture-dependent and -independent methods.					
29413003	2	16	theme	inosine	614:620	arg1	monophosphate					622:634	inosine monophosphate	614:634	inosine monophosphate	614:634	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	17	theme	monophosphate	622:634	arg1	degradation					599:609	degradation	599:609	degradation of inosine monophosphate and hypoxanthine ribonucleotide	599:666	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	8	18	theme	treated	1344:1350	arg1	samples					1352:1358	COS treated samples	1340:1358	COS treated samples	1340:1358	With the growth of Pseudomonas, COS treated samples were spoiled at day 8.					
29413003	2	19	theme	treated	365:371	arg1	samples					373:379	1% (w/v) COS treated samples	352:379	1% (w/v) COS treated samples	352:379	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	20	theme	retarding	422:430	arg1	deterioration					440:452	retarding sensory deterioration	422:452	retarding sensory deterioration	422:452	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	4	21	theme	fillets	860:866	arg1	shelf-life					834:843	shelf-life	834:843	shelf-life of silver carp fillets	834:866	Based on sensory analysis, shelf-life of silver carp fillets was 4 days for the control and 6 days for COS treated samples.					
29413003	2	22	dep	%	353:353	arg1	w/v					356:358	w/v	356:358	w/v	356:358	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	23	theme	COS	361:363	arg1	samples					373:379	1% (w/v) COS treated samples	352:379	1% (w/v) COS treated samples	352:379	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	3	24	theme	different	725:733	arg1	samples					735:741	different samples	725:741	different samples	725:741	Meanwhile, variability in the predominant microbiota in different samples was investigated by culture-dependent and -independent methods.					
29413003	0	25	dep	Hypophthalmichthys	79:96	arg1	molitrix					98:105	Hypophthalmichthys molitrix	79:105	Hypophthalmichthys molitrix	79:105	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	9	26	theme	fillets	1422:1428	arg1	quality					1411:1417	the quality	1407:1417	the quality of fillets	1407:1428	Therefore, COS improved the quality of fillets and prolonged the shelf life of silver carp fillets by 2 days during chilled storage, which was mainly due to their modulating effects on microbiota.					
29413003	3	27	theme	culture-dependent	763:779	arg1	methods					798:804	culture-dependent and -independent methods	763:804	culture-dependent and -independent methods	763:804	Meanwhile, variability in the predominant microbiota in different samples was investigated by culture-dependent and -independent methods.					
29413003	6	28	theme	Pseudomonas	1135:1145	arg1	growth					1125:1130	the growth	1121:1130	the growth of Pseudomonas, Aeromonas, and Shewanella	1121:1172	However, COS inhibited the growth of Pseudomonas, Aeromonas, and Shewanella significantly.					
29413003	0	29	theme	oligosaccharides	20:35	arg1	Effects					0:6	Effects	0:6	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix)	0:106	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	1	30	theme	microbiota	286:295	arg1	fillets					312:318	quality and microbiota of silver carp fillets	274:318	quality and microbiota of silver carp fillets stored at 4 °C	274:333	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	2	31	theme	nitrogen	535:542	arg1	production					500:509	the production	496:509	the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine	496:583	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	7	32	from	microbiota	1264:1273	arg1	samples					1290:1296	COS treated samples	1278:1296	COS treated samples	1278:1296	Consequently, Acinetobacter followed by Pseudomonas became the predominant microbiota in COS treated samples at day 6.					
29413003	9	33	theme	modulating	1546:1555	arg1	effects					1557:1563	their modulating effects	1540:1563	their modulating effects on microbiota	1540:1577	Therefore, COS improved the quality of fillets and prolonged the shelf life of silver carp fillets by 2 days during chilled storage, which was mainly due to their modulating effects on microbiota.					
29413003	0	34	theme	chitosan	11:18	arg1	oligosaccharides					20:35	chitosan oligosaccharides	11:35	chitosan oligosaccharides	11:35	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	9	35	theme	shelf	1448:1452	arg1	life					1454:1457	the shelf life	1444:1457	the shelf life of silver carp fillets by 2 days	1444:1490	Therefore, COS improved the quality of fillets and prolonged the shelf life of silver carp fillets by 2 days during chilled storage, which was mainly due to their modulating effects on microbiota.					
29413003	0	36	theme	culture-dependent	122:138	arg1	methods					156:162	culture-dependent and independent methods	122:162	culture-dependent and independent methods	122:162	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	2	37	theme	good	392:395	arg1	quality					397:403	good quality	392:403	good quality	392:403	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	0	38	theme	microbiota	40:49	arg1	composition					51:61	microbiota composition	40:61	microbiota composition of silver carp (Hypophthalmichthys molitrix)	40:106	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	1	39	theme	silver	300:305	arg1	carp					307:310	silver carp	300:310	silver carp	300:310	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	2	40	theme	volatile	520:527	arg1	putrescine					545:554	putrescine	545:554	putrescine	545:554	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	40	theme	volatile	520:527	arg1	nitrogen					535:542	total volatile basic nitrogen	514:542	total volatile basic nitrogen	514:542	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	40	theme	volatile	520:527	arg1	hypoxanthine					572:583	hypoxanthine	572:583	hypoxanthine	572:583	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	40	theme	volatile	520:527	arg1	cadaverine					557:566	cadaverine	557:566	cadaverine	557:566	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	5	41	theme	fish	1074:1077	arg1	spoilage					1079:1086	fish spoilage	1074:1086	fish spoilage	1074:1086	Meanwhile, Pseudomonas, followed by Aeromonas, Acinetobacter, and Shewanella were dominated in the control samples at day 4 and contributed to fish spoilage at day 6.					
29413003	2	42	dep	maintained	381:390	arg1	inhibiting					455:464	inhibiting	455:464	inhibiting microbial growth	455:481	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	42	dep	maintained	381:390	arg1	delaying					590:597	delaying	590:597	delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide	590:666	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	42	dep	maintained	381:390	arg1	attenuating					484:494	attenuating	484:494	attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine	484:583	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	9	43	from	effects	1557:1563	arg1	microbiota					1568:1577	microbiota	1568:1577	microbiota	1568:1577	Therefore, COS improved the quality of fillets and prolonged the shelf life of silver carp fillets by 2 days during chilled storage, which was mainly due to their modulating effects on microbiota.					
29413003	1	44	theme	chitosan	224:231	arg1	COS					251:253	COS	251:253	COS	251:253	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	1	44	theme	chitosan	224:231	arg1	oligosaccharides					233:248	chitosan oligosaccharides	224:248	chitosan oligosaccharides (COS)	224:254	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	1	45	theme	carp	307:310	arg1	microbiota					286:295	microbiota	286:295	microbiota of silver carp	286:310	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	1	45	theme	carp	307:310	arg1	quality					274:280	quality	274:280	quality	274:280	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	1	46	from	changes	263:269	arg1	fillets					312:318	quality and microbiota of silver carp fillets	274:318	quality and microbiota of silver carp fillets stored at 4 °C	274:333	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	5	47	theme	control	1030:1036	arg1	samples					1038:1044	the control samples	1026:1044	the control samples	1026:1044	Meanwhile, Pseudomonas, followed by Aeromonas, Acinetobacter, and Shewanella were dominated in the control samples at day 4 and contributed to fish spoilage at day 6.					
29413003	2	48	theme	total	514:518	arg1	putrescine					545:554	putrescine	545:554	putrescine	545:554	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	48	theme	total	514:518	arg1	nitrogen					535:542	total volatile basic nitrogen	514:542	total volatile basic nitrogen	514:542	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	48	theme	total	514:518	arg1	hypoxanthine					572:583	hypoxanthine	572:583	hypoxanthine	572:583	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	2	48	theme	total	514:518	arg1	cadaverine					557:566	cadaverine	557:566	cadaverine	557:566	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	1	49	theme	oligosaccharides	233:248	arg1	effects					213:219	the effects	209:219	the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C	209:333	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	2	50	theme	sensory	432:438	arg1	deterioration					440:452	retarding sensory deterioration	422:452	retarding sensory deterioration	422:452	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
29413003	0	51	theme	chilled	171:177	arg1	storage					179:185	chilled storage	171:185	chilled storage	171:185	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	4	52	theme	carp	855:858	arg1	fillets					860:866	silver carp fillets	848:866	silver carp fillets	848:866	Based on sensory analysis, shelf-life of silver carp fillets was 4 days for the control and 6 days for COS treated samples.					
29413003	7	53	from	day	1301:1303	arg1	microbiota					1264:1273	the predominant microbiota	1248:1273	the predominant microbiota in COS treated samples at day 6	1248:1305	Consequently, Acinetobacter followed by Pseudomonas became the predominant microbiota in COS treated samples at day 6.					
29413003	7	54	theme	treated	1282:1288	arg1	samples					1290:1296	COS treated samples	1278:1296	COS treated samples	1278:1296	Consequently, Acinetobacter followed by Pseudomonas became the predominant microbiota in COS treated samples at day 6.					
29413003	0	55	theme	independent	144:154	arg1	methods					156:162	culture-dependent and independent methods	122:162	culture-dependent and independent methods	122:162	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	3	56	theme	-independent	785:796	arg1	methods					798:804	culture-dependent and -independent methods	763:804	culture-dependent and -independent methods	763:804	Meanwhile, variability in the predominant microbiota in different samples was investigated by culture-dependent and -independent methods.					
29413003	4	57	theme	silver	848:853	arg1	fillets					860:866	silver carp fillets	848:866	silver carp fillets	848:866	Based on sensory analysis, shelf-life of silver carp fillets was 4 days for the control and 6 days for COS treated samples.					
29413003	1	58	from	effects	213:219	arg1	changes					263:269	the changes	259:269	the changes in quality and microbiota of silver carp fillets stored at 4 °C	259:333	This study evaluated the effects of chitosan oligosaccharides (COS) on the changes in quality and microbiota of silver carp fillets stored at 4 °C.					
29413003	9	59	theme	silver	1462:1467	arg1	fillets					1474:1480	silver carp fillets	1462:1480	silver carp fillets	1462:1480	Therefore, COS improved the quality of fillets and prolonged the shelf life of silver carp fillets by 2 days during chilled storage, which was mainly due to their modulating effects on microbiota.					
29413003	4	60	theme	sensory	816:822	arg1	analysis					824:831	sensory analysis	816:831	sensory analysis	816:831	Based on sensory analysis, shelf-life of silver carp fillets was 4 days for the control and 6 days for COS treated samples.					
29413003	9	61	theme	carp	1469:1472	arg1	fillets					1474:1480	silver carp fillets	1462:1480	silver carp fillets	1462:1480	Therefore, COS improved the quality of fillets and prolonged the shelf life of silver carp fillets by 2 days during chilled storage, which was mainly due to their modulating effects on microbiota.					
29413003	4	62	theme	treated	914:920	arg1	samples					922:928	COS treated samples	910:928	COS treated samples	910:928	Based on sensory analysis, shelf-life of silver carp fillets was 4 days for the control and 6 days for COS treated samples.					
29413003	7	63	theme	COS	1278:1280	arg1	samples					1290:1296	COS treated samples	1278:1296	COS treated samples	1278:1296	Consequently, Acinetobacter followed by Pseudomonas became the predominant microbiota in COS treated samples at day 6.					
29413003	0	64	theme	carp	73:76	arg1	composition					51:61	microbiota composition	40:61	microbiota composition of silver carp (Hypophthalmichthys molitrix)	40:106	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	6	65	theme	Aeromonas	1148:1156	arg1	growth					1125:1130	the growth	1121:1130	the growth of Pseudomonas, Aeromonas, and Shewanella	1121:1172	However, COS inhibited the growth of Pseudomonas, Aeromonas, and Shewanella significantly.					
29413003	7	66	theme	predominant	1252:1262	arg1	microbiota					1264:1273	the predominant microbiota	1248:1273	the predominant microbiota in COS treated samples at day 6	1248:1305	Consequently, Acinetobacter followed by Pseudomonas became the predominant microbiota in COS treated samples at day 6.					
29413003	0	67	theme	silver	66:71	arg1	Hypophthalmichthys					79:96	Hypophthalmichthys	79:96	Hypophthalmichthys	79:96	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	0	67	theme	silver	66:71	arg1	carp					73:76	silver carp	66:76	silver carp (Hypophthalmichthys molitrix)	66:106	Effects of chitosan oligosaccharides on microbiota composition of silver carp (Hypophthalmichthys molitrix) determined by culture-dependent and independent methods during chilled storage.					
29413003	3	68	theme	predominant	699:709	arg1	microbiota					711:720	the predominant microbiota	695:720	the predominant microbiota in different samples	695:741	Meanwhile, variability in the predominant microbiota in different samples was investigated by culture-dependent and -independent methods.					
29413003	4	69	dep	control	887:893	arg1	the					883:885	the	883:885	the	883:885	Based on sensory analysis, shelf-life of silver carp fillets was 4 days for the control and 6 days for COS treated samples.					
29413003	2	70	theme	%	353:353	arg1	samples					373:379	1% (w/v) COS treated samples	352:379	1% (w/v) COS treated samples	352:379	During storage, 1% (w/v) COS treated samples maintained good quality, as evidenced by retarding sensory deterioration, inhibiting microbial growth, attenuating the production of total volatile basic nitrogen, putrescine, cadaverine and hypoxanthine, and delaying degradation of inosine monophosphate and hypoxanthine ribonucleotide.					
30787611	0	0	theme	titanium	84:91	arg1	surface					73:79	the surface	69:79	the surface of titanium primed with phase-transited lysozyme	69:128	Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme.					
30787611	5	1	theme	marrow	806:811	arg1	BMSCs					828:832	BMSCs	828:832	BMSCs	828:832	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	5	1	theme	marrow	806:811	arg1	cells					821:825	bone marrow stromal cells	801:825	bone marrow stromal cells (BMSCs)	801:833	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	6	2	theme	surface	1321:1327	arg1	modification					1329:1340	surface modification	1321:1340	surface modification	1321:1340	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	3	3	theme	prime	551:555	arg1	discs					566:570	prime titanium discs	551:570	prime titanium discs with a positive charge	551:593	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	1	4	theme	implants	270:277	arg1	technology					251:260	the surface modification technology	226:260	the surface modification technology of bone implants	226:277	BACKGROUND To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly.					
30787611	5	5	theme	stromal	813:819	arg1	BMSCs					828:832	BMSCs	828:832	BMSCs	828:832	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	5	5	theme	stromal	813:819	arg1	cells					821:825	bone marrow stromal cells	801:825	bone marrow stromal cells (BMSCs)	801:833	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	8	6	theme	appropriate	1562:1572	arg1	characteristics					1582:1596	appropriate surface characteristics	1562:1596	appropriate surface characteristics	1562:1596	CONCLUSION This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant.					
30787611	9	7	theme	biomaterials	1780:1791	arg1	modification					1764:1775	the surface modification	1752:1775	the surface modification of biomaterials	1752:1791	Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials.					
30787611	3	8	theme	titanium	557:564	arg1	discs					566:570	prime titanium discs	551:570	prime titanium discs with a positive charge	551:593	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	4	9	theme	hyaluronate	616:626	arg1	nanoparticles					693:705	sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles	609:705	sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles	609:705	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	9	10	with	modification	1685:1696	arg1	ASA					1703:1705	ASA	1703:1705	ASA	1703:1705	Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials.					
30787611	3	11	with	discs	566:570	arg1	charge					588:593	a positive charge	577:593	a positive charge	577:593	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	8	12	theme	characteristics	1582:1596	arg1	effects					1551:1557	the significant effects	1535:1557	the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant	1535:1674	CONCLUSION This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant.					
30787611	6	13	theme	Ti	1304:1305	arg1	discs					1307:1311	Ti discs	1304:1311	Ti discs without surface modification	1304:1340	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	4	14	theme	Ti	736:737	arg1	discs					739:743	PTL-primed Ti discs	725:743	PTL-primed Ti discs	725:743	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	6	15	theme	macrophages	1015:1025	arg1	secretion					968:976	the secretion	964:976	the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs	964:1063	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	2	16	contain	possess	393:399	arg2	osteoimmunomodulation					411:431	favorable osteoimmunomodulation	401:431	favorable osteoimmunomodulation	401:431	Intensive research on osteoimmunomodulation has shown that the surfaces of implants should possess favorable osteoimmunomodulation to facilitate osteogenesis.					
30787611	2	16	contain	possess	393:399	arg1	surfaces					365:372	the surfaces	361:372	the surfaces of implants	361:384	Intensive research on osteoimmunomodulation has shown that the surfaces of implants should possess favorable osteoimmunomodulation to facilitate osteogenesis.					
30787611	3	17	dep	METHODS	461:467	arg1	used					543:546	used	543:546	was used to prime titanium discs with a positive charge	539:593	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	2	18	theme	Intensive	302:310	arg1	research					312:319	Intensive research	302:319	Intensive research on osteoimmunomodulation	302:344	Intensive research on osteoimmunomodulation has shown that the surfaces of implants should possess favorable osteoimmunomodulation to facilitate osteogenesis.					
30787611	5	19	theme	different	891:899	arg1	CM					920:921	CM	920:921	CM	920:921	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	5	19	theme	different	891:899	arg1	media					913:917	different conditioned media	891:917	different conditioned media (CM) generated by macrophages	891:947	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	1	20	theme	success	186:192	arg1	rate					194:197	the success rate	182:197	the success rate of implanted biomaterials	182:223	BACKGROUND To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly.					
30787611	0	21	theme	phase-transited	105:119	arg1	lysozyme					121:128	phase-transited lysozyme	105:128	phase-transited lysozyme	105:128	Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme.					
30787611	5	22	theme	cells	821:825	arg1	behaviors					788:796	The behaviors	784:796	The behaviors of bone marrow stromal cells (BMSCs)	784:833	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	1	23	dep	BACKGROUND	131:140	arg1	improve					145:151	improve	145:151	improve osseointegration	145:168	BACKGROUND To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly.					
30787611	1	23	dep	BACKGROUND	131:140	arg1	enhance					174:180	enhance	174:180	enhance the success rate of implanted biomaterials	174:223	BACKGROUND To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly.					
30787611	3	24	theme	phase-transited	498:512	arg1	PTL					524:526	PTL	524:526	PTL	524:526	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	3	24	theme	phase-transited	498:512	arg1	lysozyme					514:521	phase-transited lysozyme	498:521	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	9	25	mod	modification	1764:1775	arg1	biomaterials					1780:1791	biomaterials	1780:1791	biomaterials	1780:1791	Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials.					
30787611	9	25	mod	modification	1764:1775	arg3	surface					1756:1762	the surface modification	1752:1775	the surface modification of biomaterials	1752:1791	Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials.					
30787611	6	26	theme	macrophages	1278:1288	arg1	response					1266:1273	the inflammatory response	1249:1273	the inflammatory response of macrophages	1249:1288	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	8	27	theme	surface	1574:1580	arg1	characteristics					1582:1596	appropriate surface characteristics	1562:1596	appropriate surface characteristics	1562:1596	CONCLUSION This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant.					
30787611	3	28	theme	green	478:482	arg1	technique					529:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	6	29	theme	discs	1059:1063	arg1	surfaces					1034:1041	the surfaces	1030:1041	the surfaces of different Ti discs	1030:1063	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	5	30	contain	containing	859:868	arg1	surfaces					850:857	the Ti disc surfaces	838:857	the Ti disc surfaces containing ASA	838:872	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	5	30	contain	containing	859:868	arg2	ASA					870:872	ASA	870:872	ASA	870:872	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	1	31	theme	implanted	202:210	arg1	biomaterials					212:223	implanted biomaterials	202:223	implanted biomaterials	202:223	BACKGROUND To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly.					
30787611	5	32	theme	Ti	842:843	arg1	surfaces					850:857	the Ti disc surfaces	838:857	the Ti disc surfaces containing ASA	838:872	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	6	33	theme	cytokines	1002:1010	arg1	secretion					968:976	the secretion	964:976	the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs	964:1063	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	6	34	theme	inflammation-related	981:1000	arg1	cytokines					1002:1010	inflammation-related cytokines	981:1010	inflammation-related cytokines	981:1010	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	1	35	theme	biomaterials	212:223	arg1	rate					194:197	the success rate	182:197	the success rate of implanted biomaterials	182:223	BACKGROUND To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly.					
30787611	0	36	theme	osteoimmunomodulatory	16:36	arg1	coating					38:44	an osteoimmunomodulatory coating	13:44	an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme	13:128	Establishing an osteoimmunomodulatory coating loaded with aspirin on the surface of titanium primed with phase-transited lysozyme.					
30787611	4	37	theme	sodium	609:614	arg1	HA					629:630	HA	629:630	HA	629:630	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	4	37	theme	sodium	609:614	arg1	hyaluronate					616:626	sodium hyaluronate	609:626	sodium hyaluronate (HA)	609:631	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	4	38	theme	self-assembled	637:650	arg1	collagen					659:666	self-assembled type I collagen	637:666	self-assembled type I collagen containing aspirin (ASA)	637:691	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	5	39	from	RESULTS	776:782	arg1	behaviors					788:796	The behaviors	784:796	The behaviors of bone marrow stromal cells (BMSCs)	784:833	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	4	40	theme	PTL-primed	725:734	arg1	discs					739:743	PTL-primed Ti discs	725:743	PTL-primed Ti discs	725:743	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	4	41	theme	collagen	659:666	arg1	nanoparticles					693:705	sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles	609:705	sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles	609:705	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	3	42	theme	novel	471:475	arg1	technique					529:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	8	43	theme	osteogenesis	1619:1630	arg1	regulation					1605:1614	the regulation	1601:1614	the regulation of osteogenesis and osteoimmunomodulation around an implant	1601:1674	CONCLUSION This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant.					
30787611	6	44	contain	containing	1140:1149	arg2	ASA					1151:1153	ASA	1151:1153	ASA	1151:1153	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	6	44	contain	containing	1140:1149	arg1	surface					1132:1138	the Ti surface	1125:1138	the Ti surface containing ASA	1125:1153	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	7	45	theme	bone	1428:1431	arg1	formation					1433:1441	bone formation	1428:1441	bone formation around the implant, which mirrors the evaluation in vitro	1428:1499	After implantation in vivo, the ASA-modified implant can significantly contribute to bone formation around the implant, which mirrors the evaluation in vitro.					
30787611	5	46	theme	conditioned	901:911	arg1	CM					920:921	CM	920:921	CM	920:921	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	5	46	theme	conditioned	901:911	arg1	media					913:917	different conditioned media	891:917	different conditioned media (CM) generated by macrophages	891:947	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	3	47	theme	lysozyme	514:521	arg1	technique					529:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	3	48	theme	efficient	488:496	arg1	technique					529:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	8	49	dep	CONCLUSION	1502:1511	arg1	highlights					1524:1533	highlights	1524:1533	highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant	1524:1674	CONCLUSION This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant.					
30787611	6	50	theme	inflammatory	1253:1264	arg1	response					1266:1273	the inflammatory response	1249:1273	the inflammatory response of macrophages	1249:1288	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	1	51	theme	surface	230:236	arg1	technology					251:260	the surface modification technology	226:260	the surface modification technology of bone implants	226:277	BACKGROUND To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly.					
30787611	8	52	theme	osteoimmunomodulation	1636:1656	arg1	regulation					1605:1614	the regulation	1601:1614	the regulation of osteogenesis and osteoimmunomodulation around an implant	1601:1674	CONCLUSION This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant.					
30787611	7	53	theme	ASA-modified	1375:1386	arg1	implant					1388:1394	the ASA-modified implant	1371:1394	the ASA-modified implant	1371:1394	After implantation in vivo, the ASA-modified implant can significantly contribute to bone formation around the implant, which mirrors the evaluation in vitro.					
30787611	4	54	contain	containing	668:677	arg1	collagen					659:666	self-assembled type I collagen	637:666	self-assembled type I collagen containing aspirin (ASA)	637:691	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	4	54	contain	containing	668:677	arg2	ASA					688:690	ASA	688:690	ASA	688:690	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	4	54	contain	containing	668:677	arg2	aspirin					679:685	aspirin	679:685	aspirin (ASA)	679:691	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	8	55	theme	significant	1539:1549	arg1	effects					1551:1557	the significant effects	1535:1557	the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant	1535:1674	CONCLUSION This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant.					
30787611	2	56	theme	implants	377:384	arg1	surfaces					365:372	the surfaces	361:372	the surfaces of implants	361:384	Intensive research on osteoimmunomodulation has shown that the surfaces of implants should possess favorable osteoimmunomodulation to facilitate osteogenesis.					
30787611	6	57	dep	in	1085:1086	arg1	vitro					1088:1092	vitro	1088:1092	vitro	1088:1092	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	5	58	dep	behaviors	788:796	arg1	surfaces					850:857	the Ti disc surfaces	838:857	the Ti disc surfaces containing ASA	838:872	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	3	59	used	used	543:546	arg2	technique					529:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	A novel, green and efficient phase-transited lysozyme (PTL) technique	469:537	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	9	60	theme	surface	1756:1762	arg1	modification					1764:1775	the surface modification	1752:1775	the surface modification of biomaterials	1752:1791	Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials.					
30787611	9	61	theme	superior	1728:1735	arg1	strategies					1737:1746	superior strategies	1728:1746	superior strategies for the surface modification of biomaterials	1728:1791	Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials.					
30787611	6	62	from	secretion	968:976	arg1	surfaces					1034:1041	the surfaces	1030:1041	the surfaces of different Ti discs	1030:1063	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	9	63	theme	Implant	1677:1683	arg1	modification					1685:1696	Implant modification	1677:1696	Implant modification with ASA	1677:1705	Implant modification with ASA potentially provides superior strategies for the surface modification of biomaterials.					
30787611	1	64	theme	modification	238:249	arg1	technology					251:260	the surface modification technology	226:260	the surface modification technology of bone implants	226:277	BACKGROUND To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly.					
30787611	3	65	theme	positive	579:586	arg1	charge					588:593	a positive charge	577:593	a positive charge	577:593	METHODS A novel, green and efficient phase-transited lysozyme (PTL) technique was used to prime titanium discs with a positive charge.					
30787611	5	66	theme	disc	845:848	arg1	surfaces					850:857	the Ti disc surfaces	838:857	the Ti disc surfaces containing ASA	838:872	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	4	67	theme	electrostatic	749:761	arg1	interaction					763:773	electrostatic interaction	749:773	electrostatic interaction	749:773	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	6	68	theme	Ti	1056:1057	arg1	discs					1059:1063	different Ti discs	1046:1063	different Ti discs	1046:1063	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	8	69	from	effects	1551:1557	arg1	regulation					1605:1614	the regulation	1601:1614	the regulation of osteogenesis and osteoimmunomodulation around an implant	1601:1674	CONCLUSION This study highlights the significant effects of appropriate surface characteristics on the regulation of osteogenesis and osteoimmunomodulation around an implant.					
30787611	6	70	theme	BMSCs	1226:1230	arg1	proliferation					1189:1201	proliferation	1189:1201	proliferation	1189:1201	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	6	70	theme	BMSCs	1226:1230	arg1	migration					1178:1186	the migration	1174:1186	the migration	1174:1186	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	6	70	theme	BMSCs	1226:1230	arg1	differentiation					1207:1221	differentiation	1207:1221	differentiation	1207:1221	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	6	71	theme	in	1085:1086	arg1	experiments					1094:1104	in vitro experiments	1085:1104	in vitro experiments	1085:1104	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	6	72	theme	Ti	1129:1130	arg1	surface					1132:1138	the Ti surface	1125:1138	the Ti surface containing ASA	1125:1153	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	2	73	from	research	312:319	arg1	osteoimmunomodulation					324:344	osteoimmunomodulation	324:344	osteoimmunomodulation	324:344	Intensive research on osteoimmunomodulation has shown that the surfaces of implants should possess favorable osteoimmunomodulation to facilitate osteogenesis.					
30787611	6	74	theme	different	1046:1054	arg1	discs					1059:1063	different Ti discs	1046:1063	different Ti discs	1046:1063	Additionally, the secretion of inflammation-related cytokines of macrophages on the surfaces of different Ti discs was investigated in in vitro experiments, which showed that the Ti surface containing ASA not only supported the migration, proliferation and differentiation of BMSCs but also reduced the inflammatory response of macrophages compared with Ti discs without surface modification.					
30787611	5	75	theme	bone	801:804	arg1	marrow					806:811	bone marrow	801:811	bone marrow stromal cells (BMSCs)	801:833	RESULTS The behaviors of bone marrow stromal cells (BMSCs) on the Ti disc surfaces containing ASA were analyzed in different conditioned media (CM) generated by macrophages.					
30787611	4	76	theme	type	652:655	arg1	collagen					659:666	self-assembled type I collagen	637:666	self-assembled type I collagen containing aspirin (ASA)	637:691	In addition, sodium hyaluronate (HA) and self-assembled type I collagen containing aspirin (ASA) nanoparticles were decorated on PTL-primed Ti discs via electrostatic interaction.					
30787611	2	77	theme	favorable	401:409	arg1	osteoimmunomodulation					411:431	favorable osteoimmunomodulation	401:431	favorable osteoimmunomodulation	401:431	Intensive research on osteoimmunomodulation has shown that the surfaces of implants should possess favorable osteoimmunomodulation to facilitate osteogenesis.					
30787611	1	78	theme	bone	265:268	arg1	implants					270:277	bone implants	265:277	bone implants	265:277	BACKGROUND To improve osseointegration and enhance the success rate of implanted biomaterials, the surface modification technology of bone implants has developed rapidly.					
31626822	10	0	theme	ASCs	1293:1296	arg1	potential					1276:1284	the angiogenic potential	1261:1284	the angiogenic potential of the ASCs	1261:1296	Among the groups treated with different NPs, the angiogenic potential of the ASCs was most robust in the ST-Fe2O3-treated group.					
31626822	10	0	theme	ASCs	1293:1296	arg1	robust					1307:1312	robust	1307:1312	robust	1307:1312	Among the groups treated with different NPs, the angiogenic potential of the ASCs was most robust in the ST-Fe2O3-treated group.					
31626822	1	1	theme	cells	159:163	arg1	labelling					141:149	labelling	141:149	labelling of stem cells using nanoparticles (NPs) for tracking purpose	141:210	However, labelling of stem cells using nanoparticles (NPs) for tracking purpose has been intensively investigated, the biosafety of these materials needs more clarification.					
31626822	8	2	theme	cells	1072:1076	arg1	capacity					1060:1067	the migration capacity	1046:1067	the migration capacity of cells labelled with Fe2O3, Fe3O4 and CoxNi1-xFe2O4	1046:1121	Compared to that of the TA-treated cells, the migration capacity of cells labelled with Fe2O3, Fe3O4 and CoxNi1-xFe2O4 was significantly compromised.					
31626822	11	3	theme	angiogenic	1421:1430	arg1	potential					1432:1440	angiogenic potential	1421:1440	angiogenic potential	1421:1440	In conclusion, labelling ASCs with ST-Fe2O3 NPs enhanced cell migration and angiogenic potential and conferred higher resistance to apoptosis than labelling the cells with the other tested NPs.					
31626822	2	4	theme	Fe2O3	344:348	arg1	forms					324:328	different forms	314:328	different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated)	314:424	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	0	5	theme	stem	112:115	arg1	tracking					122:129	stem cell tracking	112:129	stem cell tracking	112:129	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	10	6	theme	ST-Fe2O3-treated	1321:1336	arg1	group					1338:1342	the ST-Fe2O3-treated group	1317:1342	the ST-Fe2O3-treated group	1317:1342	Among the groups treated with different NPs, the angiogenic potential of the ASCs was most robust in the ST-Fe2O3-treated group.					
31626822	11	7	theme	higher	1456:1461	arg1	resistance					1463:1472	higher resistance	1456:1472	higher resistance to apoptosis	1456:1485	In conclusion, labelling ASCs with ST-Fe2O3 NPs enhanced cell migration and angiogenic potential and conferred higher resistance to apoptosis than labelling the cells with the other tested NPs.					
31626822	2	8	dep	Fe2O3	344:348	arg1	either					381:386	either	381:386	either	381:386	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	3	9	theme	adipose-derived	466:480	arg1	ASCs					494:497	ASCs	494:497	ASCs	494:497	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	3	9	theme	adipose-derived	466:480	arg1	cells					487:491	adipose-derived stem cells	466:491	adipose-derived stem cells (ASCs)	466:498	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	3	10	theme	stem	482:485	arg1	ASCs					494:497	ASCs	494:497	ASCs	494:497	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	3	10	theme	stem	482:485	arg1	cells					487:491	adipose-derived stem cells	466:491	adipose-derived stem cells (ASCs)	466:498	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	4	11	dep	cell	626:629	arg1	proliferation					631:643	proliferation	631:643	proliferation	631:643	We then evaluated the effect of these NPs on stem cell proliferation, viability, migration and angiogenesis.					
31626822	4	11	dep	cell	626:629	arg1	angiogenesis					671:682	angiogenesis	671:682	angiogenesis	671:682	We then evaluated the effect of these NPs on stem cell proliferation, viability, migration and angiogenesis.					
31626822	4	11	dep	cell	626:629	arg1	migration					657:665	migration	657:665	migration	657:665	We then evaluated the effect of these NPs on stem cell proliferation, viability, migration and angiogenesis.					
31626822	4	11	dep	cell	626:629	arg1	viability					646:654	viability	646:654	viability	646:654	We then evaluated the effect of these NPs on stem cell proliferation, viability, migration and angiogenesis.					
31626822	11	12	theme	ST-Fe2O3	1380:1387	arg1	NPs					1389:1391	ST-Fe2O3 NPs	1380:1391	ST-Fe2O3 NPs	1380:1391	In conclusion, labelling ASCs with ST-Fe2O3 NPs enhanced cell migration and angiogenic potential and conferred higher resistance to apoptosis than labelling the cells with the other tested NPs.					
31626822	2	13	theme	NPs	377:379	arg1	forms					324:328	different forms	314:328	different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated)	314:424	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	0	14	theme	cell	117:120	arg1	tracking					122:129	stem cell tracking	112:129	stem cell tracking	112:129	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	11	15	theme	labelling	1360:1368	arg1	ASCs					1370:1373	labelling ASCs	1360:1373	labelling ASCs with ST-Fe2O3 NPs	1360:1391	In conclusion, labelling ASCs with ST-Fe2O3 NPs enhanced cell migration and angiogenic potential and conferred higher resistance to apoptosis than labelling the cells with the other tested NPs.					
31626822	2	16	theme	Fe2O4	371:375	arg1	NPs					377:379	CoxNi1-x Fe2O4 NPs	362:379	CoxNi1-x Fe2O4 NPs	362:379	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	11	17	theme	other	1521:1525	arg1	NPs					1534:1536	the other tested NPs	1517:1536	the other tested NPs	1517:1536	In conclusion, labelling ASCs with ST-Fe2O3 NPs enhanced cell migration and angiogenic potential and conferred higher resistance to apoptosis than labelling the cells with the other tested NPs.					
31626822	2	18	theme	different	314:322	arg1	forms					324:328	different forms	314:328	different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated)	314:424	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	7	19	theme	cell	984:987	arg1	proliferation					989:1001	cell proliferation	984:1001	cell proliferation	984:1001	Conversely, CoxNi1-x Fe2O4 NPs caused a significant decrease in cell proliferation.					
31626822	2	20	theme	CoxNi1-x	362:369	arg1	NPs					377:379	CoxNi1-x Fe2O4 NPs	362:379	CoxNi1-x Fe2O4 NPs	362:379	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	1	21	theme	materials	270:278	arg1	biosafety					251:259	the biosafety	247:259	the biosafety of these materials	247:278	However, labelling of stem cells using nanoparticles (NPs) for tracking purpose has been intensively investigated, the biosafety of these materials needs more clarification.					
31626822	4	22	from	effect	598:603	arg1	cell					626:629	stem cell proliferation, viability, migration and angiogenesis	621:682	stem cell proliferation, viability, migration and angiogenesis	621:682	We then evaluated the effect of these NPs on stem cell proliferation, viability, migration and angiogenesis.					
31626822	4	23	theme	NPs	614:616	arg1	effect					598:603	the effect	594:603	the effect of these NPs on stem cell proliferation, viability, migration and angiogenesis	594:682	We then evaluated the effect of these NPs on stem cell proliferation, viability, migration and angiogenesis.					
31626822	0	24	theme	uncoated	24:31	arg1	nanoparticles					80:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	3	25	dep	the	521:523	arg1	aid					525:527	aid	525:527	aid	525:527	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	0	26	theme	different	14:22	arg1	nanoparticles					80:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	11	27	theme	tested	1527:1532	arg1	NPs					1534:1536	the other tested NPs	1517:1536	the other tested NPs	1517:1536	In conclusion, labelling ASCs with ST-Fe2O3 NPs enhanced cell migration and angiogenic potential and conferred higher resistance to apoptosis than labelling the cells with the other tested NPs.					
31626822	5	28	theme	TA-treated	837:846	arg1	cells					848:852	TA-treated cells	837:852	TA-treated cells	837:852	Results showed that ASCs labelled with Fe2O3, Fe3O4, ST-Fe2O3 and ST-Fe3O4 did not show any significant difference in proliferation compared to that of TA-treated cells.					
31626822	1	29	theme	tracking	195:202	arg1	purpose					204:210	tracking purpose	195:210	tracking purpose	195:210	However, labelling of stem cells using nanoparticles (NPs) for tracking purpose has been intensively investigated, the biosafety of these materials needs more clarification.					
31626822	0	30	theme	starch-coated	37:49	arg1	nanoparticles					80:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	6	31	theme	protective	883:892	arg1	effect					894:899	a protective effect	881:899	a protective effect against apoptosis	881:917	Moreover, they have shown a protective effect against apoptosis.					
31626822	8	32	theme	TA-treated	1028:1037	arg1	cells					1039:1043	the TA-treated cells	1024:1043	the TA-treated cells	1024:1043	Compared to that of the TA-treated cells, the migration capacity of cells labelled with Fe2O3, Fe3O4 and CoxNi1-xFe2O4 was significantly compromised.					
31626822	9	33	theme	ST-coated	1173:1181	arg1	composites					1183:1192	the ST-coated composites	1169:1192	the ST-coated composites	1169:1192	Interestingly, the ST-coated composites reversed this effect.					
31626822	2	34	dep	uncoated	388:395	arg1	ST-coated					415:423	ST-coated	415:423	ST-coated	415:423	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	10	35	theme	different	1246:1254	arg1	NPs					1256:1258	different NPs	1246:1258	different NPs	1246:1258	Among the groups treated with different NPs, the angiogenic potential of the ASCs was most robust in the ST-Fe2O3-treated group.					
31626822	2	36	theme	uncoated	388:395	arg1	forms					324:328	different forms	314:328	different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated)	314:424	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	3	37	with	NPs	512:514	arg1	the					521:523	the	521:523	the	521:523	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	5	38	theme	significant	777:787	arg1	difference					789:798	any significant difference	773:798	any significant difference in proliferation compared to that of TA-treated cells	773:852	Results showed that ASCs labelled with Fe2O3, Fe3O4, ST-Fe2O3 and ST-Fe3O4 did not show any significant difference in proliferation compared to that of TA-treated cells.					
31626822	0	39	theme	iron	69:72	arg1	nanoparticles					80:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	10	40	from	robust	1307:1312	arg1	group					1338:1342	the ST-Fe2O3-treated group	1317:1342	the ST-Fe2O3-treated group	1317:1342	Among the groups treated with different NPs, the angiogenic potential of the ASCs was most robust in the ST-Fe2O3-treated group.					
31626822	8	41	theme	migration	1050:1058	arg1	capacity					1060:1067	the migration capacity	1046:1067	the migration capacity of cells labelled with Fe2O3, Fe3O4 and CoxNi1-xFe2O4	1046:1121	Compared to that of the TA-treated cells, the migration capacity of cells labelled with Fe2O3, Fe3O4 and CoxNi1-xFe2O4 was significantly compromised.					
31626822	2	42	theme	oxide	338:342	arg1	Fe2O3					344:348	iron oxide Fe2O3	333:348	iron oxide Fe2O3	333:348	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	0	43	theme	superparamagnetic	51:67	arg1	nanoparticles					80:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	11	44	with	labelling	1492:1500	arg1	NPs					1534:1536	the other tested NPs	1517:1536	the other tested NPs	1517:1536	In conclusion, labelling ASCs with ST-Fe2O3 NPs enhanced cell migration and angiogenic potential and conferred higher resistance to apoptosis than labelling the cells with the other tested NPs.					
31626822	3	45	link	adipose-derived	466:480	arg1	ASCs					494:497	ASCs	494:497	ASCs	494:497	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	3	45	link	adipose-derived	466:480	arg1	cells					487:491	adipose-derived stem cells	466:491	adipose-derived stem cells (ASCs)	466:498	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	3	46	theme	transfection	551:562	arg1	agent					564:568	a transfection agent	549:568	a transfection agent (TA)	549:573	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	3	46	theme	transfection	551:562	arg1	NPs					512:514	these NPs	506:514	these NPs with the aid of lipofectamine	506:544	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	3	46	theme	transfection	551:562	arg1	TA					571:572	TA	571:572	TA	571:572	We successfully labelled adipose-derived stem cells (ASCs) using these NPs with the aid of lipofectamine as a transfection agent (TA).					
31626822	7	47	theme	Fe2O4	941:945	arg1	NPs					947:949	CoxNi1-x Fe2O4 NPs	932:949	CoxNi1-x Fe2O4 NPs	932:949	Conversely, CoxNi1-x Fe2O4 NPs caused a significant decrease in cell proliferation.					
31626822	2	48	theme	iron	333:336	arg1	Fe2O3					344:348	iron oxide Fe2O3	333:348	iron oxide Fe2O3	333:348	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31626822	7	49	theme	significant	960:970	arg1	decrease					972:979	a significant decrease	958:979	a significant decrease in cell proliferation	958:1001	Conversely, CoxNi1-x Fe2O4 NPs caused a significant decrease in cell proliferation.					
31626822	11	50	with	ASCs	1370:1373	arg1	NPs					1389:1391	ST-Fe2O3 NPs	1380:1391	ST-Fe2O3 NPs	1380:1391	In conclusion, labelling ASCs with ST-Fe2O3 NPs enhanced cell migration and angiogenic potential and conferred higher resistance to apoptosis than labelling the cells with the other tested NPs.					
31626822	4	51	theme	stem	621:624	arg1	cell					626:629	stem cell proliferation, viability, migration and angiogenesis	621:682	stem cell proliferation, viability, migration and angiogenesis	621:682	We then evaluated the effect of these NPs on stem cell proliferation, viability, migration and angiogenesis.					
31626822	10	52	from	group	1338:1342	arg1	potential					1276:1284	the angiogenic potential	1261:1284	the angiogenic potential of the ASCs	1261:1296	Among the groups treated with different NPs, the angiogenic potential of the ASCs was most robust in the ST-Fe2O3-treated group.					
31626822	10	52	from	group	1338:1342	arg1	robust					1307:1312	robust	1307:1312	robust	1307:1312	Among the groups treated with different NPs, the angiogenic potential of the ASCs was most robust in the ST-Fe2O3-treated group.					
31626822	10	53	theme	angiogenic	1265:1274	arg1	potential					1276:1284	the angiogenic potential	1261:1284	the angiogenic potential of the ASCs	1261:1296	Among the groups treated with different NPs, the angiogenic potential of the ASCs was most robust in the ST-Fe2O3-treated group.					
31626822	10	53	theme	angiogenic	1265:1274	arg1	robust					1307:1312	robust	1307:1312	robust	1307:1312	Among the groups treated with different NPs, the angiogenic potential of the ASCs was most robust in the ST-Fe2O3-treated group.					
31626822	1	54	theme	stem	154:157	arg1	cells					159:163	stem cells	154:163	stem cells	154:163	However, labelling of stem cells using nanoparticles (NPs) for tracking purpose has been intensively investigated, the biosafety of these materials needs more clarification.					
31626822	7	55	from	decrease	972:979	arg1	proliferation					989:1001	cell proliferation	984:1001	cell proliferation	984:1001	Conversely, CoxNi1-x Fe2O4 NPs caused a significant decrease in cell proliferation.					
31626822	0	56	theme	nanoparticles	80:92	arg1	Comparison					0:9	Comparison	0:9	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.	0:130	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	7	57	theme	CoxNi1-x	932:939	arg1	NPs					947:949	CoxNi1-x Fe2O4 NPs	932:949	CoxNi1-x Fe2O4 NPs	932:949	Conversely, CoxNi1-x Fe2O4 NPs caused a significant decrease in cell proliferation.					
31626822	11	58	theme	cell	1402:1405	arg1	migration					1407:1415	cell migration	1402:1415	cell migration	1402:1415	In conclusion, labelling ASCs with ST-Fe2O3 NPs enhanced cell migration and angiogenic potential and conferred higher resistance to apoptosis than labelling the cells with the other tested NPs.					
31626822	0	59	theme	oxide	74:78	arg1	nanoparticles					80:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	different uncoated and starch-coated superparamagnetic iron oxide nanoparticles	14:92	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	0	60	dep	Comparison	0:9	arg1	Implications					95:106	Implications	95:106	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.	0:130	Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking.					
31626822	5	61	from	difference	789:798	arg1	proliferation					803:815	proliferation	803:815	proliferation	803:815	Results showed that ASCs labelled with Fe2O3, Fe3O4, ST-Fe2O3 and ST-Fe3O4 did not show any significant difference in proliferation compared to that of TA-treated cells.					
31626822	2	62	theme	Fe3O4	351:355	arg1	forms					324:328	different forms	314:328	different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated)	314:424	Herein, different forms of iron oxide Fe2O3, Fe3O4, and CoxNi1-x Fe2O4 NPs either uncoated or starch-coated (ST-coated) were prepared.					
31279475	8	0	theme	monolithic	1709:1718	arg1	columns					1720:1726	the prepared monolithic columns	1696:1726	the prepared monolithic columns	1696:1726	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	1	1	theme	sequential	313:322	arg1	reaction					324:331	one-pot sequential reaction	305:331	one-pot sequential reaction for the first time	305:350	In this study, a hydroxypropyl-β-cyclodextrin (HP-β-CD) functionalized monolithic capillary column was prepared by one-pot sequential reaction for the first time.					
31279475	2	2	dep	DBU	590:592	arg1	2					597:597	2	597:597	2	597:597	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	2	dep	DBU	590:592	arg1	copolymerization					604:619	the copolymerization	600:619	1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD	554:634	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	3	3	theme	columns	747:753	arg1	series					726:731	A series	724:731	A series of monolithic columns	724:753	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	4	4	theme	adsorption	1162:1171	arg1	analysis					1173:1180	nitrogen adsorption analysis	1153:1180	nitrogen adsorption analysis	1153:1180	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	9	5	theme	strategy	1928:1935	arg1	potential					1867:1875	potential	1867:1875	potential	1867:1875	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	8	6	theme	efficiency	1824:1833	arg1	reproducibilities					1765:1781	reproducibilities	1765:1781	reproducibilities	1765:1781	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	8	6	theme	efficiency	1824:1833	arg1	stability					1751:1759	satisfactory stability	1738:1759	satisfactory stability	1738:1759	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	6	7	theme	stationary	1298:1307	arg1	CSP					1316:1318	CSP	1316:1318	CSP	1316:1318	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	6	7	theme	stationary	1298:1307	arg1	phase					1309:1313	the prepared chiral stationary phase (CSP)	1278:1319	the prepared chiral stationary phase (CSP)	1278:1319	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	6	8	theme	racemic	1364:1370	arg1	compounds					1372:1380	racemic compounds	1364:1380	racemic compounds	1364:1380	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	6	9	theme	chiral	1291:1296	arg1	CSP					1316:1318	CSP	1316:1318	CSP	1316:1318	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	6	9	theme	chiral	1291:1296	arg1	phase					1309:1313	the prepared chiral stationary phase (CSP)	1278:1319	the prepared chiral stationary phase (CSP)	1278:1319	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	6	10	theme	set	1357:1359	arg1	separation					1341:1350	the separation	1337:1350	the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates	1337:1516	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	1	11	theme	capillary	272:280	arg1	column					282:287	a hydroxypropyl-β-cyclodextrin (HP-β-CD) functionalized monolithic capillary column	205:287	a hydroxypropyl-β-cyclodextrin (HP-β-CD) functionalized monolithic capillary column	205:287	In this study, a hydroxypropyl-β-cyclodextrin (HP-β-CD) functionalized monolithic capillary column was prepared by one-pot sequential reaction for the first time.					
31279475	3	12	theme	reaction	829:836	arg1	temperature					797:807	the temperature	793:807	the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature)	793:985	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	13	dep	parameters	866:875	arg1	composition					891:901	composition	891:901	composition	891:901	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	13	dep	parameters	866:875	arg1	parameters					866:875	several copolymerization parameters	841:875	several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature)	841:985	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	13	dep	parameters	866:875	arg1	type					882:885	type	882:885	type	882:885	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	9	14	theme	sequential	1917:1926	arg1	strategy					1928:1935	the developed one-pot sequential strategy	1895:1935	the developed one-pot sequential strategy	1895:1935	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	3	15	theme	ring	816:819	arg1	reaction					829:836	the ring opening reaction	812:836	the ring opening reaction	812:836	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	0	16	theme	one-pot	82:88	arg1	reaction					101:108	one-pot sequential reaction	82:108	one-pot sequential reaction	82:108	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	3	17	theme	solvents	916:923	arg1	composition					891:901	composition	891:901	composition	891:901	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	17	theme	solvents	916:923	arg1	parameters					866:875	several copolymerization parameters	841:875	several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature)	841:985	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	17	theme	solvents	916:923	arg1	type					882:885	type	882:885	type	882:885	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	7	18	theme	flavanone	1653:1661	arg1	epimers					1674:1680	four flavanone glycosides epimers	1648:1680	four flavanone glycosides epimers	1648:1680	Additionally, the column also showed excellent separation abilities towards four flavanone glycosides epimers.					
31279475	3	19	theme	parameters	866:875	arg1	temperature					797:807	the temperature	793:807	the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature)	793:985	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	2	20	theme	undec-7-ene	577:587	arg1	DBU					590:592	1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD	554:634	1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD	554:634	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	21	theme	2-acrylamido-2-methyl	672:692	arg1	acid					711:714	2-acrylamido-2-methyl propane sulfonic acid	672:714	2-acrylamido-2-methyl propane sulfonic acid (AMPS)	672:721	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	21	theme	2-acrylamido-2-methyl	672:692	arg1	AMPS					717:720	AMPS	717:720	AMPS	717:720	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	3	22	theme	several	841:847	arg1	composition					891:901	composition	891:901	composition	891:901	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	22	theme	several	841:847	arg1	parameters					866:875	several copolymerization parameters	841:875	several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature)	841:985	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	22	theme	several	841:847	arg1	type					882:885	type	882:885	type	882:885	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	23	theme	GMA-HP-β-CD	935:945	arg1	ratio					926:930	ratio	926:930	ratio of GMA-HP-β-CD to EDMA	926:953	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	23	theme	GMA-HP-β-CD	935:945	arg1	solvents					916:923	porogenic solvents	906:923	porogenic solvents	906:923	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	23	theme	GMA-HP-β-CD	935:945	arg1	temperature					974:984	polymerization temperature	959:984	polymerization temperature	959:984	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	7	24	theme	separation	1619:1628	arg1	abilities					1630:1638	excellent separation abilities	1609:1638	excellent separation abilities	1609:1638	Additionally, the column also showed excellent separation abilities towards four flavanone glycosides epimers.					
31279475	9	25	theme	developed	1899:1907	arg1	strategy					1928:1935	the developed one-pot sequential strategy	1895:1935	the developed one-pot sequential strategy	1895:1935	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	3	26	theme	ratio	926:930	arg1	composition					891:901	composition	891:901	composition	891:901	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	26	theme	ratio	926:930	arg1	parameters					866:875	several copolymerization parameters	841:875	several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature)	841:985	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	26	theme	ratio	926:930	arg1	type					882:885	type	882:885	type	882:885	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	4	27	theme	phases	1065:1070	arg1	morphologies					998:1009	morphologies	998:1009	morphologies	998:1009	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	4	27	theme	phases	1065:1070	arg1	structures					1015:1024	structures	1015:1024	structures	1015:1024	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	5	28	theme	successful	1224:1233	arg1	bonding					1235:1241	the successful bonding	1220:1241	the successful bonding of HP-β-CD onto the monolith	1220:1270	Raman spectroscopy clearly indicated the successful bonding of HP-β-CD onto the monolith.					
31279475	2	29	dep	reaction	484:491	arg1	1					464:464	1	464:464	1	464:464	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	3	30	theme	polymerization	959:972	arg1	temperature					974:984	polymerization temperature	959:984	polymerization temperature	959:984	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	2	31	theme	opening	476:482	arg1	reaction					484:491	the ring opening reaction	467:491	(1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS)	463:721	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	32	theme	sulfonic	702:709	arg1	acid					711:714	2-acrylamido-2-methyl propane sulfonic acid	672:714	2-acrylamido-2-methyl propane sulfonic acid (AMPS)	672:721	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	32	theme	sulfonic	702:709	arg1	AMPS					717:720	AMPS	717:720	AMPS	717:720	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	6	33	theme	β-adrenergic	1465:1476	arg1	drugs					1478:1482	β-adrenergic drugs	1465:1482	β-adrenergic drugs	1465:1482	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	0	34	theme	functionalized	46:59	arg1	column					72:77	a hydroxypropyl-β-cyclodextrin functionalized monolithic column	15:77	a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction	15:108	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	4	35	theme	monolithic	1043:1052	arg1	phases					1065:1070	the resulting monolithic stationary phases	1029:1070	the resulting monolithic stationary phases	1029:1070	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	2	36	theme	column	410:415	arg1	preparation					357:367	The preparation	353:367	The preparation of the HP-β-CD functionalized monolithic column	353:415	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	0	37	theme	column	72:77	arg1	application					118:128	its application	114:128	its application for capillary electrochromatographic enantiomer separation	114:187	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	0	37	theme	column	72:77	arg1	Preparation					0:10	Preparation	0:10	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction	0:108	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	9	38	theme	CD	1977:1978	arg1	columns					2006:2012	other derivatized CD functionalized monolithic columns	1959:2012	other derivatized CD functionalized monolithic columns	1959:2012	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	2	39	theme	functionalized	384:397	arg1	column					410:415	the HP-β-CD functionalized monolithic column	372:415	the HP-β-CD functionalized monolithic column	372:415	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	8	40	theme	satisfactory	1738:1749	arg1	stability					1751:1759	satisfactory stability	1738:1759	satisfactory stability	1738:1759	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	9	41	theme	monolithic	1995:2004	arg1	columns					2006:2012	other derivatized CD functionalized monolithic columns	1959:2012	other derivatized CD functionalized monolithic columns	1959:2012	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	6	42	theme	satisfactory	1519:1530	arg1	selectivities					1543:1555	satisfactory separation selectivities	1519:1555	satisfactory separation selectivities	1519:1555	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	9	43	theme	other	1959:1963	arg1	columns					2006:2012	other derivatized CD functionalized monolithic columns	1959:2012	other derivatized CD functionalized monolithic columns	1959:2012	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	4	44	theme	optical	1094:1100	arg1	microscopy					1102:1111	optical microscopy	1094:1111	optical microscopy	1094:1111	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	1	45	theme	one-pot	305:311	arg1	reaction					324:331	one-pot sequential reaction	305:331	one-pot sequential reaction for the first time	305:350	In this study, a hydroxypropyl-β-cyclodextrin (HP-β-CD) functionalized monolithic capillary column was prepared by one-pot sequential reaction for the first time.					
31279475	9	46	from	preparation	1944:1954	arg1	potential					1867:1875	potential	1867:1875	potential	1867:1875	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	2	47	theme	GMA-HP-β-CD	624:634	arg1	AMPS					717:720	AMPS	717:720	AMPS	717:720	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	47	theme	GMA-HP-β-CD	624:634	arg1	acid					711:714	2-acrylamido-2-methyl propane sulfonic acid	672:714	2-acrylamido-2-methyl propane sulfonic acid (AMPS)	672:721	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	47	theme	GMA-HP-β-CD	624:634	arg1	EDMA					662:665	EDMA	662:665	EDMA	662:665	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	47	theme	GMA-HP-β-CD	624:634	arg1	DBU					590:592	1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD	554:634	1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD	554:634	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	47	theme	GMA-HP-β-CD	624:634	arg1	dimethacrylate					646:659	ethylene dimethacrylate	637:659	ethylene dimethacrylate (EDMA)	637:666	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	8	48	theme	prepared	1700:1707	arg1	columns					1720:1726	the prepared monolithic columns	1696:1726	the prepared monolithic columns	1696:1726	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	8	49	theme	time	1796:1799	arg1	reproducibilities					1765:1781	reproducibilities	1765:1781	reproducibilities	1765:1781	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	8	49	theme	time	1796:1799	arg1	stability					1751:1759	satisfactory stability	1738:1759	satisfactory stability	1738:1759	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	8	50	theme	resolution	1802:1811	arg1	reproducibilities					1765:1781	reproducibilities	1765:1781	reproducibilities	1765:1781	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	8	50	theme	resolution	1802:1811	arg1	stability					1751:1759	satisfactory stability	1738:1759	satisfactory stability	1738:1759	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	6	51	theme	racemic	1434:1440	arg1	drugs					1458:1462	racemic anticholinergic drugs	1434:1462	racemic anticholinergic drugs	1434:1462	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	3	52	theme	monolithic	736:745	arg1	columns					747:753	monolithic columns	736:753	monolithic columns	736:753	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	8	53	theme	column	1817:1822	arg1	efficiency					1824:1833	column efficiency	1817:1833	column efficiency	1817:1833	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31279475	2	54	theme	ethylene	637:644	arg1	dimethacrylate					646:659	ethylene dimethacrylate	637:659	ethylene dimethacrylate (EDMA)	637:666	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	54	theme	ethylene	637:644	arg1	EDMA					662:665	EDMA	662:665	EDMA	662:665	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	55	from	reactions	441:449	arg1	pot					458:460	one pot	454:460	one pot	454:460	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	0	56	theme	enantiomer	167:176	arg1	separation					178:187	capillary electrochromatographic enantiomer separation	134:187	capillary electrochromatographic enantiomer separation	134:187	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	4	57	theme	nitrogen	1153:1160	arg1	analysis					1173:1180	nitrogen adsorption analysis	1153:1180	nitrogen adsorption analysis	1153:1180	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	1	58	theme	monolithic	261:270	arg1	column					282:287	a hydroxypropyl-β-cyclodextrin (HP-β-CD) functionalized monolithic capillary column	205:287	a hydroxypropyl-β-cyclodextrin (HP-β-CD) functionalized monolithic capillary column	205:287	In this study, a hydroxypropyl-β-cyclodextrin (HP-β-CD) functionalized monolithic capillary column was prepared by one-pot sequential reaction for the first time.					
31279475	4	59	theme	electron	1123:1130	arg1	SEM					1144:1146	SEM	1144:1146	SEM	1144:1146	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	4	59	theme	electron	1123:1130	arg1	microscopy					1132:1141	scanning electron microscopy	1114:1141	scanning electron microscopy (SEM)	1114:1147	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	6	60	theme	prepared	1282:1289	arg1	CSP					1316:1318	CSP	1316:1318	CSP	1316:1318	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	6	60	theme	prepared	1282:1289	arg1	phase					1309:1313	the prepared chiral stationary phase (CSP)	1278:1319	the prepared chiral stationary phase (CSP)	1278:1319	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	6	61	theme	compounds	1372:1380	arg1	set					1357:1359	a set	1355:1359	a set of racemic compounds	1355:1380	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	6	61	theme	compounds	1372:1380	arg1	compounds					1372:1380	racemic compounds	1364:1380	racemic compounds	1364:1380	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	4	62	dep	morphologies	998:1009	arg1	the					994:996	the	994:996	the	994:996	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	7	63	theme	glycosides	1663:1672	arg1	epimers					1674:1680	four flavanone glycosides epimers	1648:1680	four flavanone glycosides epimers	1648:1680	Additionally, the column also showed excellent separation abilities towards four flavanone glycosides epimers.					
31279475	0	64	theme	sequential	90:99	arg1	reaction					101:108	one-pot sequential reaction	82:108	one-pot sequential reaction	82:108	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	9	65	theme	functionalized	1980:1993	arg1	columns					2006:2012	other derivatized CD functionalized monolithic columns	1959:2012	other derivatized CD functionalized monolithic columns	1959:2012	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	3	66	theme	opening	821:827	arg1	reaction					829:836	the ring opening reaction	812:836	the ring opening reaction	812:836	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	0	67	theme	electrochromatographic	144:165	arg1	separation					178:187	capillary electrochromatographic enantiomer separation	134:187	capillary electrochromatographic enantiomer separation	134:187	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	2	68	theme	propane	694:700	arg1	acid					711:714	2-acrylamido-2-methyl propane sulfonic acid	672:714	2-acrylamido-2-methyl propane sulfonic acid (AMPS)	672:721	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	68	theme	propane	694:700	arg1	AMPS					717:720	AMPS	717:720	AMPS	717:720	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	5	69	theme	Raman	1183:1187	arg1	spectroscopy					1189:1200	Raman spectroscopy	1183:1200	Raman spectroscopy	1183:1200	Raman spectroscopy clearly indicated the successful bonding of HP-β-CD onto the monolith.					
31279475	3	70	dep	type	882:885	arg1	the					878:880	the	878:880	the	878:880	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	71	theme	porogenic	906:914	arg1	solvents					916:923	porogenic solvents	906:923	porogenic solvents	906:923	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	7	72	theme	excellent	1609:1617	arg1	abilities					1630:1638	excellent separation abilities	1609:1638	excellent separation abilities	1609:1638	Additionally, the column also showed excellent separation abilities towards four flavanone glycosides epimers.					
31279475	2	73	dep	involves	417:424	arg1	reaction					484:491	the ring opening reaction	467:491	(1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS)	463:721	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	3	74	theme	copolymerization	849:864	arg1	composition					891:901	composition	891:901	composition	891:901	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	74	theme	copolymerization	849:864	arg1	parameters					866:875	several copolymerization parameters	841:875	several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature)	841:985	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	74	theme	copolymerization	849:864	arg1	type					882:885	type	882:885	type	882:885	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	0	75	theme	capillary	134:142	arg1	separation					178:187	capillary electrochromatographic enantiomer separation	134:187	capillary electrochromatographic enantiomer separation	134:187	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	9	76	theme	one-pot	1909:1915	arg1	strategy					1928:1935	the developed one-pot sequential strategy	1895:1935	the developed one-pot sequential strategy	1895:1935	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	6	77	theme	capillary	1385:1393	arg1	electrochromatography					1395:1415	capillary electrochromatography	1385:1415	capillary electrochromatography (CEC)	1385:1421	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	6	77	theme	capillary	1385:1393	arg1	CEC					1418:1420	CEC	1418:1420	CEC	1418:1420	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	4	78	theme	stationary	1054:1063	arg1	phases					1065:1070	the resulting monolithic stationary phases	1029:1070	the resulting monolithic stationary phases	1029:1070	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	0	79	theme	hydroxypropyl-β-cyclodextrin	17:44	arg1	column					72:77	a hydroxypropyl-β-cyclodextrin functionalized monolithic column	15:77	a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction	15:108	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	1	80	theme	first	341:345	arg1	time					347:350	the first time	337:350	the first time	337:350	In this study, a hydroxypropyl-β-cyclodextrin (HP-β-CD) functionalized monolithic capillary column was prepared by one-pot sequential reaction for the first time.					
31279475	3	81	theme	temperature	974:984	arg1	composition					891:901	composition	891:901	composition	891:901	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	81	theme	temperature	974:984	arg1	parameters					866:875	several copolymerization parameters	841:875	several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature)	841:985	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	3	81	theme	temperature	974:984	arg1	type					882:885	type	882:885	type	882:885	A series of monolithic columns were successfully prepared by varying the temperature of the ring opening reaction or several copolymerization parameters (the type and composition of porogenic solvents, ratio of GMA-HP-β-CD to EDMA and polymerization temperature).					
31279475	9	82	theme	derivatized	1965:1975	arg1	columns					2006:2012	other derivatized CD functionalized monolithic columns	1959:2012	other derivatized CD functionalized monolithic columns	1959:2012	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	2	83	theme	ring	471:474	arg1	reaction					484:491	the ring opening reaction	467:491	(1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS)	463:721	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	0	84	theme	monolithic	61:70	arg1	column					72:77	a hydroxypropyl-β-cyclodextrin functionalized monolithic column	15:77	a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction	15:108	Preparation of a hydroxypropyl-β-cyclodextrin functionalized monolithic column by one-pot sequential reaction and its application for capillary electrochromatographic enantiomer separation.					
31279475	5	85	theme	HP-β-CD	1246:1252	arg1	bonding					1235:1241	the successful bonding	1220:1241	the successful bonding of HP-β-CD onto the monolith	1220:1270	Raman spectroscopy clearly indicated the successful bonding of HP-β-CD onto the monolith.					
31279475	2	86	theme	glycidyl	513:520	arg1	GMA					536:538	GMA	536:538	GMA	536:538	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	2	86	theme	glycidyl	513:520	arg1	methacrylate					522:533	glycidyl methacrylate	513:533	glycidyl methacrylate (GMA)	513:539	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	6	87	theme	anticholinergic	1442:1456	arg1	drugs					1458:1462	racemic anticholinergic drugs	1434:1462	racemic anticholinergic drugs	1434:1462	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	4	88	theme	resulting	1033:1041	arg1	phases					1065:1070	the resulting monolithic stationary phases	1029:1070	the resulting monolithic stationary phases	1029:1070	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	2	89	theme	monolithic	399:408	arg1	column					410:415	the HP-β-CD functionalized monolithic column	372:415	the HP-β-CD functionalized monolithic column	372:415	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	4	90	theme	scanning	1114:1121	arg1	SEM					1144:1146	SEM	1144:1146	SEM	1144:1146	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	4	90	theme	scanning	1114:1121	arg1	microscopy					1132:1141	scanning electron microscopy	1114:1141	scanning electron microscopy (SEM)	1114:1147	Then, the morphologies and structures of the resulting monolithic stationary phases were characterized by optical microscopy, scanning electron microscopy (SEM) and nitrogen adsorption analysis.					
31279475	2	91	theme	HP-β-CD	376:382	arg1	column					410:415	the HP-β-CD functionalized monolithic column	372:415	the HP-β-CD functionalized monolithic column	372:415	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	6	92	theme	separation	1532:1541	arg1	selectivities					1543:1555	satisfactory separation selectivities	1519:1555	satisfactory separation selectivities	1519:1555	When the prepared chiral stationary phase (CSP) was applied for the separation of a set of racemic compounds by capillary electrochromatography (CEC), including racemic anticholinergic drugs, β-adrenergic drugs, meptazinol and its intermediates, satisfactory separation selectivities were obtained.					
31279475	9	93	from	potential	1867:1875	arg1	preparation					1944:1954	the preparation	1940:1954	the preparation of other derivatized CD functionalized monolithic columns	1940:2012	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	9	94	theme	columns	2006:2012	arg1	preparation					1944:1954	the preparation	1940:1954	the preparation of other derivatized CD functionalized monolithic columns	1940:2012	These results demonstrated the potential and usefulness of the developed one-pot sequential strategy in the preparation of other derivatized CD functionalized monolithic columns.					
31279475	2	95	theme	sequential	430:439	arg1	reactions					441:449	two sequential reactions	426:449	two sequential reactions in one pot	426:460	The preparation of the HP-β-CD functionalized monolithic column involves two sequential reactions in one pot: (1) the ring opening reaction between HP-β-CD and glycidyl methacrylate (GMA) catalyzed by 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU); (2) the copolymerization of GMA-HP-β-CD, ethylene dimethacrylate (EDMA) and 2-acrylamido-2-methyl propane sulfonic acid (AMPS).					
31279475	8	96	theme	retention	1786:1794	arg1	time					1796:1799	retention time	1786:1799	retention time	1786:1799	Furthermore, the prepared monolithic columns exhibited satisfactory stability and reproducibilities of retention time, resolution and column efficiency.					
31052157	0	0	from	Patterns	24:31	arg1	Community					75:83	the Gut Microbial Community	57:83	the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide	57:150	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	7	1	theme	blood	1104:1108	arg1	nitrogen					1115:1122	blood urea nitrogen	1104:1122	blood urea nitrogen levels	1104:1129	For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels (r = 0.90 and -0.75, respectively).					
31052157	2	2	theme	theory	357:362	arg1	approach					370:377	a random matrix theory based approach	341:377	a random matrix theory based approach	341:377	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	4	3	theme	Thirty-four	594:604	arg1	connectors					675:684	connectors	675:684	connectors	675:684	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	3	theme	Thirty-four	594:604	arg1	OTU					635:637	OTU	635:637	OTU	635:637	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	3	theme	Thirty-four	594:604	arg1	hubs					666:669	module hubs	659:669	module hubs	659:669	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	3	theme	Thirty-four	594:604	arg1	Units					628:632	Thirty-four Operational Taxonomic Units	594:632	Thirty-four Operational Taxonomic Units (OTU)	594:638	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	2	4	theme	healthy	469:475	arg1	conditions					490:499	healthy and stressed conditions	469:499	healthy and stressed conditions	469:499	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	7	5	theme	urea	1110:1113	arg1	nitrogen					1115:1122	blood urea nitrogen	1104:1122	blood urea nitrogen levels	1104:1129	For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels (r = 0.90 and -0.75, respectively).					
31052157	1	6	theme	microbial	163:171	arg1	interactions					173:184	microbial interactions	163:184	microbial interactions	163:184	Detecting microbial interactions is essential to the understanding of the structure and function of the gut microbiome.					
31052157	9	7	theme	novel	1447:1451	arg1	insights					1453:1460	novel insights	1447:1460	novel insights into gut microbial interactions	1447:1492	Our findings provided novel insights into gut microbial interactions and may facilitate future endeavor in microbial community engineering.					
31052157	1	8	theme	gut	257:259	arg1	microbiome					261:270	the gut microbiome	253:270	the gut microbiome	253:270	Detecting microbial interactions is essential to the understanding of the structure and function of the gut microbiome.					
31052157	2	9	theme	matrix	350:355	arg1	approach					370:377	a random matrix theory based approach	341:377	a random matrix theory based approach	341:377	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	3	10	theme	network	536:542	arg1	composition					544:554	the network composition and microbial co-occurrence patterns	532:591	composition	544:554	The exercise stress disrupted the network composition and microbial co-occurrence patterns.					
31052157	2	11	theme	microbial	288:296	arg1	patterns					312:319	microbial co-occurrence patterns	288:319	microbial co-occurrence patterns	288:319	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	1	12	theme	microbiome	261:270	arg1	function					241:248	function	241:248	function	241:248	Detecting microbial interactions is essential to the understanding of the structure and function of the gut microbiome.					
31052157	1	12	theme	microbiome	261:270	arg1	structure					227:235	structure	227:235	structure	227:235	Detecting microbial interactions is essential to the understanding of the structure and function of the gut microbiome.					
31052157	0	13	theme	Mice	88:91	arg1	Community					75:83	the Gut Microbial Community	57:83	the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide	57:150	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	8	14	with	Proteobacteria	1240:1253	arg1	activities					1281:1290	superoxide dismutase activities	1260:1290	superoxide dismutase activities	1260:1290	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	9	15	theme	future	1513:1518	arg1	endeavor					1520:1527	future endeavor	1513:1527	future endeavor in microbial community engineering	1513:1562	Our findings provided novel insights into gut microbial interactions and may facilitate future endeavor in microbial community engineering.					
31052157	8	16	theme	=	1308:1308	arg1	OTUs					1223:1226	the OTUs	1219:1226	the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05)	1219:1324	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	8	16	theme	=	1308:1308	arg1	r					1306:1306	r	1306:1306	r = 0.57	1306:1313	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	4	17	theme	Operational	606:616	arg1	connectors					675:684	connectors	675:684	connectors	675:684	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	17	theme	Operational	606:616	arg1	OTU					635:637	OTU	635:637	OTU	635:637	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	17	theme	Operational	606:616	arg1	hubs					666:669	module hubs	659:669	module hubs	659:669	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	17	theme	Operational	606:616	arg1	Units					628:632	Thirty-four Operational Taxonomic Units	594:632	Thirty-four Operational Taxonomic Units (OTU)	594:638	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	8	18	theme	superoxide	1260:1269	arg1	dismutase					1271:1279	superoxide dismutase	1260:1279	superoxide dismutase activities	1260:1290	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	8	19	theme	OTUs	1223:1226	arg1	connectivity					1203:1214	node connectivity	1198:1214	node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05)	1198:1324	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	5	20	dep	host	914:917	arg1	the					910:912	the	910:912	the	910:912	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	7	21	dep	Modules	1024:1030	arg1	stress					1060:1065	stress + CSD	1060:1071	stress	1060:1065	For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels (r = 0.90 and -0.75, respectively).					
31052157	7	21	dep	Modules	1024:1030	arg1	CSD					1069:1071	stress + CSD	1060:1071	CSD	1069:1071	For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels (r = 0.90 and -0.75, respectively).					
31052157	7	21	dep	Modules	1024:1030	arg1	M02					1032:1034	M02	1032:1034	M02	1032:1034	For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels (r = 0.90 and -0.75, respectively).					
31052157	7	21	dep	Modules	1024:1030	arg1	S05					1055:1057	S05	1055:1057	S05	1055:1057	For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels (r = 0.90 and -0.75, respectively).					
31052157	7	21	dep	Modules	1024:1030	arg1	Modules					1024:1030	Modules M02 (under stress) and S05 (stress + CSD)	1024:1072	Modules M02 (under stress) and S05 (stress + CSD)	1024:1072	For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels (r = 0.90 and -0.75, respectively).					
31052157	5	22	theme	stressed	797:804	arg1	network					806:812	the stressed network	793:812	the stressed network in response to CSD supplement	793:842	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	8	23	dep	assigned	1228:1235	arg1	OTUs					1223:1226	the OTUs	1219:1226	the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05)	1219:1324	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	8	23	dep	assigned	1228:1235	arg1	r					1306:1306	r	1306:1306	r = 0.57	1306:1313	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	0	24	from	Species	46:52	arg1	Community					75:83	the Gut Microbial Community	57:83	the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide	57:150	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	0	25	theme	Microbial	0:8	arg1	Patterns					24:31	Microbial Co-Occurrence Patterns	0:31	Microbial Co-Occurrence Patterns	0:31	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	2	26	theme	gut	386:388	arg1	microbiome					390:399	the gut microbiome	382:399	the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD)	382:461	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	0	27	theme	Co-Occurrence	10:22	arg1	Patterns					24:31	Microbial Co-Occurrence Patterns	0:31	Microbial Co-Occurrence Patterns	0:31	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	3	28	theme	co-occurrence	570:582	arg1	patterns					584:591	the network composition and microbial co-occurrence patterns	532:591	patterns	584:591	The exercise stress disrupted the network composition and microbial co-occurrence patterns.					
31052157	4	29	theme	module	659:664	arg1	Units					628:632	Thirty-four Operational Taxonomic Units	594:632	Thirty-four Operational Taxonomic Units (OTU)	594:638	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	29	theme	module	659:664	arg1	hubs					666:669	module hubs	659:669	module hubs	659:669	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	2	30	theme	sulfate	436:442	arg1	CSD					458:460	CSD	458:460	CSD	458:460	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	2	30	theme	sulfate	436:442	arg1	disaccharide					444:455	chondroitin sulfate disaccharide	424:455	chondroitin sulfate disaccharide (CSD)	424:461	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	3	31	theme	microbial	560:568	arg1	patterns					584:591	the network composition and microbial co-occurrence patterns	532:591	patterns	584:591	The exercise stress disrupted the network composition and microbial co-occurrence patterns.					
31052157	5	32	theme	key	859:861	arg1	schaedleri					758:767	Mucispirillum schaedleri	744:767	Mucispirillum schaedleri	744:767	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	5	32	theme	key	859:861	arg1	role					863:866	a key role	857:866	a key role	857:866	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	5	32	theme	key	859:861	arg1	connector					780:788	a connector	778:788	a connector in the stressed network in response to CSD supplement	778:842	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	5	33	from	connector	780:788	arg1	network					806:812	the stressed network	793:812	the stressed network in response to CSD supplement	793:842	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	2	34	theme	chondroitin	424:434	arg1	CSD					458:460	CSD	458:460	CSD	458:460	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	2	34	theme	chondroitin	424:434	arg1	disaccharide					444:455	chondroitin sulfate disaccharide	424:455	chondroitin sulfate disaccharide (CSD)	424:461	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	8	35	theme	pathological	1404:1415	arg1	marker					1417:1422	a potential pathological marker	1392:1422	a potential pathological marker	1392:1422	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	8	35	theme	pathological	1404:1415	arg1	Proteobacteria					1357:1370	Proteobacteria	1357:1370	Proteobacteria	1357:1370	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	2	36	theme	co-occurrence	298:310	arg1	patterns					312:319	microbial co-occurrence patterns	288:319	microbial co-occurrence patterns	288:319	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	9	37	theme	community	1542:1550	arg1	engineering					1552:1562	microbial community engineering	1532:1562	microbial community engineering	1532:1562	Our findings provided novel insights into gut microbial interactions and may facilitate future endeavor in microbial community engineering.					
31052157	6	38	theme	physiological	971:983	arg1	parameters					985:994	physiological parameters	971:994	physiological parameters	971:994	Several modules correlated with physiological parameters were detected.					
31052157	8	39	theme	p	1316:1316	arg1	<					1318:1318	p < 0.05	1316:1323	p < 0.05	1316:1323	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	8	40	theme	potential	1394:1402	arg1	marker					1417:1422	a potential pathological marker	1392:1422	a potential pathological marker	1392:1422	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	8	40	theme	potential	1394:1402	arg1	Proteobacteria					1357:1370	Proteobacteria	1357:1370	Proteobacteria	1357:1370	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	0	41	dep	Stress	108:113	arg1	Disaccharide					139:150	Disaccharide	139:150	Disaccharide	139:150	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	5	42	theme	intimate	880:887	arg1	interactions					889:900	intimate interactions	880:900	intimate interactions between the host and its microbiome	880:936	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	8	43	theme	positive	1169:1176	arg1	correlation					1178:1188	A positive correlation	1167:1188	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05)	1167:1324	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	9	44	theme	microbial	1532:1540	arg1	engineering					1552:1562	microbial community engineering	1532:1562	microbial community engineering	1532:1562	Our findings provided novel insights into gut microbial interactions and may facilitate future endeavor in microbial community engineering.					
31052157	0	45	from	Community	75:83	arg1	Response					96:103	Response	96:103	Response to Stress and Chondroitin Sulfate Disaccharide	96:150	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	8	46	dep	r	1306:1306	arg1	<					1318:1318	p < 0.05	1316:1323	p < 0.05	1316:1323	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	2	47	theme	mice	404:407	arg1	microbiome					390:399	the gut microbiome	382:399	the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD)	382:461	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	0	48	theme	Keystone	37:44	arg1	Species					46:52	Keystone Species	37:52	Keystone Species	37:52	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	0	49	theme	Chondroitin	119:129	arg1	Sulfate					131:137	Chondroitin Sulfate	119:137	Chondroitin Sulfate	119:137	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	1	50	dep	structure	227:235	arg1	the					223:225	the	223:225	the	223:225	Detecting microbial interactions is essential to the understanding of the structure and function of the gut microbiome.					
31052157	5	51	theme	CSD	829:831	arg1	supplement					833:842	CSD supplement	829:842	CSD supplement	829:842	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	8	52	theme	dismutase	1271:1279	arg1	activities					1281:1290	superoxide dismutase activities	1260:1290	superoxide dismutase activities	1260:1290	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	7	53	theme	nitrogen	1115:1122	arg1	levels					1124:1129	blood urea nitrogen levels	1104:1129	blood urea nitrogen levels	1104:1129	For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels (r = 0.90 and -0.75, respectively).					
31052157	2	54	theme	stressed	481:488	arg1	conditions					490:499	healthy and stressed conditions	469:499	healthy and stressed conditions	469:499	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	2	55	theme	random	343:348	arg1	approach					370:377	a random matrix theory based approach	341:377	a random matrix theory based approach	341:377	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	9	56	theme	gut	1467:1469	arg1	interactions					1481:1492	gut microbial interactions	1467:1492	gut microbial interactions	1467:1492	Our findings provided novel insights into gut microbial interactions and may facilitate future endeavor in microbial community engineering.					
31052157	6	57	theme	Several	939:945	arg1	modules					947:953	Several modules	939:953	Several modules correlated with physiological parameters	939:994	Several modules correlated with physiological parameters were detected.					
31052157	4	58	theme	Taxonomic	618:626	arg1	connectors					675:684	connectors	675:684	connectors	675:684	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	58	theme	Taxonomic	618:626	arg1	OTU					635:637	OTU	635:637	OTU	635:637	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	58	theme	Taxonomic	618:626	arg1	hubs					666:669	module hubs	659:669	module hubs	659:669	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	4	58	theme	Taxonomic	618:626	arg1	Units					628:632	Thirty-four Operational Taxonomic Units	594:632	Thirty-four Operational Taxonomic Units (OTU)	594:638	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	2	59	from	microbiome	390:399	arg1	response					412:419	response	412:419	response to chondroitin sulfate disaccharide (CSD)	412:461	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	8	60	theme	further	1335:1341	arg1	evidence					1343:1350	further evidence that Proteobacteria can be developed as a potential pathological marker	1335:1422	further evidence that Proteobacteria can be developed as a potential pathological marker	1335:1422	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	1	61	theme	structure	227:235	arg1	understanding					206:218	the understanding	202:218	the understanding of the structure and function of the gut microbiome	202:270	Detecting microbial interactions is essential to the understanding of the structure and function of the gut microbiome.					
31052157	9	62	from	endeavor	1520:1527	arg1	engineering					1552:1562	microbial community engineering	1532:1562	microbial community engineering	1532:1562	Our findings provided novel insights into gut microbial interactions and may facilitate future endeavor in microbial community engineering.					
31052157	4	63	theme	microbial	723:731	arg1	community					733:741	the microbial community	719:741	the microbial community	719:741	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	0	64	theme	Microbial	65:73	arg1	Community					75:83	the Gut Microbial Community	57:83	the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide	57:150	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	8	65	dep	=	1308:1308	arg1	0.57					1310:1313	0.57	1310:1313	0.57	1310:1313	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	9	66	theme	microbial	1471:1479	arg1	interactions					1481:1492	gut microbial interactions	1467:1492	gut microbial interactions	1467:1492	Our findings provided novel insights into gut microbial interactions and may facilitate future endeavor in microbial community engineering.					
31052157	3	67	theme	exercise	506:513	arg1	stress					515:520	The exercise stress	502:520	The exercise stress	502:520	The exercise stress disrupted the network composition and microbial co-occurrence patterns.					
31052157	8	68	theme	node	1198:1201	arg1	connectivity					1203:1214	node connectivity	1198:1214	node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05)	1198:1324	A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress (r = 0.57, p < 0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker.					
31052157	1	69	theme	function	241:248	arg1	understanding					206:218	the understanding	202:218	the understanding of the structure and function of the gut microbiome	202:270	Detecting microbial interactions is essential to the understanding of the structure and function of the gut microbiome.					
31052157	0	70	theme	Gut	61:63	arg1	Community					75:83	the Gut Microbial Community	57:83	the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide	57:150	Microbial Co-Occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide.					
31052157	5	71	from	network	806:812	arg1	response					817:824	response	817:824	response to CSD supplement	817:842	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	2	72	theme	based	364:368	arg1	approach					370:377	a random matrix theory based approach	341:377	a random matrix theory based approach	341:377	In this study, microbial co-occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions.					
31052157	4	73	from	generalists	704:714	arg1	community					733:741	the microbial community	719:741	the microbial community	719:741	Thirty-four Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community.					
31052157	5	74	theme	Mucispirillum	744:756	arg1	schaedleri					758:767	Mucispirillum schaedleri	744:767	Mucispirillum schaedleri	744:767	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	5	74	theme	Mucispirillum	744:756	arg1	role					863:866	a key role	857:866	a key role	857:866	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31052157	5	74	theme	Mucispirillum	744:756	arg1	connector					780:788	a connector	778:788	a connector in the stressed network in response to CSD supplement	778:842	Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role in bridging intimate interactions between the host and its microbiome.					
31184123	3	0	theme	condensed	470:478	arg1	tannins					480:486	condensed tannins	470:486	condensed tannins	470:486	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	6	1	theme	procyanidin	884:894	arg1	dimer					903:907	procyanidin B-type dimer	884:907	procyanidin B-type dimer	884:907	Among them, procyanidin B-type dimer, caffeoylquinic-acid-hexoside, and quercetin-hexose-deoxyhexose have not been previously reported in noni bagasse, noni juice, or noni fruit.					
31184123	0	2	theme	Carotenoids	86:96	arg1	Analysis					0:7	Analysis	0:7	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds	0:48	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	2	theme	Carotenoids	86:96	arg1	Determination					69:81	HPLC-DAD-Based Determination	54:81	HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse	54:137	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	2	3	from	fruit	425:429	arg1	period					352:357	a 1 week period	343:357	a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit	343:429	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	3	4	theme	tannins	480:486	arg1	Totals					432:437	Totals	432:437	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals	432:525	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	6	5	theme	noni	1010:1013	arg1	bagasse					1015:1021	noni bagasse	1010:1021	noni bagasse	1010:1021	Among them, procyanidin B-type dimer, caffeoylquinic-acid-hexoside, and quercetin-hexose-deoxyhexose have not been previously reported in noni bagasse, noni juice, or noni fruit.					
31184123	0	6	from	Analysis	0:7	arg1	Bagasse					131:137	Bagasse	131:137	Bagasse	131:137	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	6	from	Analysis	0:7	arg1	Morinda					108:114	Morinda	108:114	Morinda	108:114	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	6	from	Analysis	0:7	arg1	Noni					101:104	Noni	101:104	Noni	101:104	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	3	7	theme	most	506:509	arg1	Totals					432:437	Totals	432:437	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals	432:525	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	6	8	theme	noni	1039:1042	arg1	fruit					1044:1048	noni fruit	1039:1048	noni fruit	1039:1048	Among them, procyanidin B-type dimer, caffeoylquinic-acid-hexoside, and quercetin-hexose-deoxyhexose have not been previously reported in noni bagasse, noni juice, or noni fruit.					
31184123	3	9	theme	insoluble	642:650	arg1	fiber					660:664	total and insoluble dietary fiber	632:664	total and insoluble dietary fiber	632:664	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	0	10	dep	Morinda	108:114	arg1	L.					127:128	Morinda citrifolia L.	108:128	Morinda citrifolia L.	108:128	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	6	11	theme	noni	1024:1027	arg1	juice					1029:1033	noni juice	1024:1033	noni juice	1024:1033	Among them, procyanidin B-type dimer, caffeoylquinic-acid-hexoside, and quercetin-hexose-deoxyhexose have not been previously reported in noni bagasse, noni juice, or noni fruit.					
31184123	7	12	theme	abundant	1093:1100	arg1	compound					1102:1109	the most abundant compound	1084:1109	the most abundant compound in both bagasses	1084:1126	Isorhamnetin-3- O-rutinoside was the most abundant compound in both bagasses.					
31184123	7	12	theme	abundant	1093:1100	arg1	O-rutinoside					1067:1078	Isorhamnetin-3- O-rutinoside	1051:1078	Isorhamnetin-3- O-rutinoside	1051:1078	Isorhamnetin-3- O-rutinoside was the most abundant compound in both bagasses.					
31184123	3	13	theme	dietary	652:658	arg1	fiber					660:664	total and insoluble dietary fiber	632:664	total and insoluble dietary fiber	632:664	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	2	14	theme	drip-extraction	381:395	arg1	process					397:403	a short-term juice drip-extraction process	362:403	a short-term juice drip-extraction process	362:403	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	2	15	theme	phytochemical	261:273	arg1	composition					275:285	the phytochemical composition	257:285	the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit	257:429	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	3	16	theme	total	632:636	arg1	fiber					660:664	total and insoluble dietary fiber	632:664	total and insoluble dietary fiber	632:664	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	2	17	theme	noni	420:423	arg1	fruit					425:429	over-ripe noni fruit	410:429	over-ripe noni fruit	410:429	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	8	18	theme	potential	1162:1170	arg1	bagasses					1149:1156	both bagasses	1144:1156	both bagasses	1144:1156	In conclusion, both bagasses are potential sources of phytochemical compounds for the food and pharmaceutical industries.					
31184123	8	18	theme	potential	1162:1170	arg1	sources					1172:1178	potential sources	1162:1178	potential sources of phytochemical compounds for the food and pharmaceutical industries	1162:1248	In conclusion, both bagasses are potential sources of phytochemical compounds for the food and pharmaceutical industries.					
31184123	1	19	theme	noni	181:184	arg1	process					203:209	the noni juice extraction process	177:209	the noni juice extraction process	177:209	Noni bagasse is usually wasted after the noni juice extraction process.					
31184123	2	20	theme	over-ripe	410:418	arg1	fruit					425:429	over-ripe noni fruit	410:429	over-ripe noni fruit	410:429	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	8	21	theme	food	1215:1218	arg1	industries					1239:1248	the food and pharmaceutical industries	1211:1248	the food and pharmaceutical industries	1211:1248	In conclusion, both bagasses are potential sources of phytochemical compounds for the food and pharmaceutical industries.					
31184123	2	22	theme	bagasse	295:301	arg1	composition					275:285	the phytochemical composition	257:285	the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit	257:429	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	3	23	with	bagasse	578:584	arg1	WSB					598:600	WSB	598:600	WSB	598:600	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	3	23	with	bagasse	578:584	arg1	seeds					591:595	seeds	591:595	seeds (WSB)	591:601	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	2	24	theme	process	397:403	arg1	period					352:357	a 1 week period	343:357	a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit	343:429	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	2	25	theme	noni	290:293	arg1	bagasse					295:301	noni bagasse	290:301	noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit	290:429	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	3	26	dep	higher	532:537	arg1	whereas					604:610	whereas	604:610	whereas	604:610	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	1	27	theme	extraction	192:201	arg1	process					203:209	the noni juice extraction process	177:209	the noni juice extraction process	177:209	Noni bagasse is usually wasted after the noni juice extraction process.					
31184123	0	28	theme	Compounds	40:48	arg1	UPLC-DAD-ESI-MS					12:26	UPLC-DAD-ESI-MS	12:26	UPLC-DAD-ESI-MS of Phenolic Compounds	12:48	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	29	from	Carotenoids	86:96	arg1	Bagasse					131:137	Bagasse	131:137	Bagasse	131:137	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	29	from	Carotenoids	86:96	arg1	Morinda					108:114	Morinda	108:114	Morinda	108:114	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	29	from	Carotenoids	86:96	arg1	Noni					101:104	Noni	101:104	Noni	101:104	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	2	30	theme	week	347:350	arg1	period					352:357	a 1 week period	343:357	a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit	343:429	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	2	31	theme	juice	375:379	arg1	process					397:403	a short-term juice drip-extraction process	362:403	a short-term juice drip-extraction process	362:403	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	0	32	theme	Phenolic	31:38	arg1	Compounds					40:48	Phenolic Compounds	31:48	Phenolic Compounds	31:48	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	7	33	theme	Isorhamnetin-3-	1051:1065	arg1	compound					1102:1109	the most abundant compound	1084:1109	the most abundant compound in both bagasses	1084:1126	Isorhamnetin-3- O-rutinoside was the most abundant compound in both bagasses.					
31184123	7	33	theme	Isorhamnetin-3-	1051:1065	arg1	O-rutinoside					1067:1078	Isorhamnetin-3- O-rutinoside	1051:1078	Isorhamnetin-3- O-rutinoside	1051:1078	Isorhamnetin-3- O-rutinoside was the most abundant compound in both bagasses.					
31184123	8	34	theme	phytochemical	1183:1195	arg1	compounds					1197:1205	phytochemical compounds	1183:1205	phytochemical compounds	1183:1205	In conclusion, both bagasses are potential sources of phytochemical compounds for the food and pharmaceutical industries.					
31184123	2	35	theme	short-term	364:373	arg1	process					397:403	a short-term juice drip-extraction process	362:403	a short-term juice drip-extraction process	362:403	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	0	36	theme	HPLC-DAD-Based	54:67	arg1	Determination					69:81	HPLC-DAD-Based Determination	54:81	HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse	54:137	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	37	from	Determination	69:81	arg1	Bagasse					131:137	Bagasse	131:137	Bagasse	131:137	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	37	from	Determination	69:81	arg1	Morinda					108:114	Morinda	108:114	Morinda	108:114	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	37	from	Determination	69:81	arg1	Noni					101:104	Noni	101:104	Noni	101:104	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	3	38	theme	flavonoids	458:467	arg1	Totals					432:437	Totals	432:437	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals	432:525	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	3	39	theme	bound	612:616	arg1	phenolics					618:626	bound phenolics	612:626	bound phenolics	612:626	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	8	40	theme	compounds	1197:1205	arg1	bagasses					1149:1156	both bagasses	1144:1156	both bagasses	1144:1156	In conclusion, both bagasses are potential sources of phytochemical compounds for the food and pharmaceutical industries.					
31184123	8	40	theme	compounds	1197:1205	arg1	sources					1172:1178	potential sources	1162:1178	potential sources of phytochemical compounds for the food and pharmaceutical industries	1162:1248	In conclusion, both bagasses are potential sources of phytochemical compounds for the food and pharmaceutical industries.					
31184123	1	41	theme	Noni	140:143	arg1	bagasse					145:151	Noni bagasse	140:151	Noni bagasse	140:151	Noni bagasse is usually wasted after the noni juice extraction process.					
31184123	7	42	from	compound	1102:1109	arg1	bagasses					1119:1126	both bagasses	1114:1126	both bagasses	1114:1126	Isorhamnetin-3- O-rutinoside was the most abundant compound in both bagasses.					
31184123	3	43	theme	phenolics	447:455	arg1	Totals					432:437	Totals	432:437	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals	432:525	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	5	44	theme	iridoids	827:834	arg1	total					790:794	A total	788:794	A total of 16 phenolic compounds and 2 iridoids	788:834	A total of 16 phenolic compounds and 2 iridoids were determined by UPLC-DAD-ESI-MS.					
31184123	8	45	theme	pharmaceutical	1224:1237	arg1	industries					1239:1248	the food and pharmaceutical industries	1211:1248	the food and pharmaceutical industries	1211:1248	In conclusion, both bagasses are potential sources of phytochemical compounds for the food and pharmaceutical industries.					
31184123	5	46	theme	compounds	811:819	arg1	total					790:794	A total	788:794	A total of 16 phenolic compounds and 2 iridoids	788:834	A total of 16 phenolic compounds and 2 iridoids were determined by UPLC-DAD-ESI-MS.					
31184123	3	47	theme	free	442:445	arg1	phenolics					447:455	free phenolics	442:455	free phenolics	442:455	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	5	48	theme	phenolic	802:809	arg1	compounds					811:819	16 phenolic compounds	799:819	16 phenolic compounds	799:819	A total of 16 phenolic compounds and 2 iridoids were determined by UPLC-DAD-ESI-MS.					
31184123	0	49	from	Noni	101:104	arg1	Analysis					0:7	Analysis	0:7	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds	0:48	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	0	49	from	Noni	101:104	arg1	Determination					69:81	HPLC-DAD-Based Determination	54:81	HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse	54:137	Analysis by UPLC-DAD-ESI-MS of Phenolic Compounds and HPLC-DAD-Based Determination of Carotenoids in Noni ( Morinda citrifolia L.) Bagasse.					
31184123	1	50	theme	juice	186:190	arg1	process					203:209	the noni juice extraction process	177:209	the noni juice extraction process	177:209	Noni bagasse is usually wasted after the noni juice extraction process.					
31184123	2	51	theme	1	345:345	arg1	week					347:350	week	347:350	week	347:350	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	2	52	theme	study	232:236	arg1	purpose					216:222	The purpose	212:222	The purpose of this study	212:236	The purpose of this study was to investigate the phytochemical composition of noni bagasse (with and without seeds) obtained after a 1 week period of a short-term juice drip-extraction process from over-ripe noni fruit.					
31184123	8	53	from	sources	1172:1178	arg1	conclusion					1132:1141	conclusion	1132:1141	conclusion	1132:1141	In conclusion, both bagasses are potential sources of phytochemical compounds for the food and pharmaceutical industries.					
31184123	3	54	theme	carotenoids	489:499	arg1	Totals					432:437	Totals	432:437	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals	432:525	Totals of free phenolics, flavonoids, condensed tannins, carotenoids, and most of the minerals were higher in bagasse without seeds (NSB) than in bagasse with seeds (WSB), whereas bound phenolics and total and insoluble dietary fiber were higher in WSB than in NSB.					
31184123	6	55	theme	B-type	896:901	arg1	dimer					903:907	procyanidin B-type dimer	884:907	procyanidin B-type dimer	884:907	Among them, procyanidin B-type dimer, caffeoylquinic-acid-hexoside, and quercetin-hexose-deoxyhexose have not been previously reported in noni bagasse, noni juice, or noni fruit.					
30244042	5	0	theme	control	853:859	arg1	group					861:865	An identically comprised reference control group	818:865	An identically comprised reference control group	818:865	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	3	1	from	tests	526:530	arg1	rats					604:607	neonatal Sprague-Dawley rats	580:607	neonatal Sprague-Dawley rats	580:607	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	5	2	theme	LNT	1039:1041	arg1	group					1043:1047	the high-dose LNT group	1025:1047	the high-dose LNT group	1025:1047	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	6	3	dep	in	1079:1080	arg1	vitro					1082:1086	vitro	1082:1086	vitro	1082:1086	LNT was non-genotoxic in the in vitro tests.					
30244042	5	4	theme	direct	999:1004	arg1	comparison					1006:1015	direct comparison	999:1015	direct comparison against the high-dose LNT group	999:1047	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	3	5	theme	toxicity	561:568	arg1	study					570:574	a subchronic oral gavage toxicity study	536:574	a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats)	536:608	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	5	theme	toxicity	561:568	arg1	part					466:469	part	466:469	part of the safety evaluation of LNT	466:501	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	5	theme	toxicity	561:568	arg1	tests					526:530	in vitro genotoxicity tests	504:530	in vitro genotoxicity tests	504:530	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	6	from	study	570:574	arg1	rats					604:607	neonatal Sprague-Dawley rats	580:607	neonatal Sprague-Dawley rats	580:607	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	4	7	theme	weight	724:729	arg1	/day					735:738	0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day	690:738	/day	735:738	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	3	8	dep	in	504:505	arg1	vitro					507:511	vitro	507:511	vitro	507:511	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	2	9	theme	infant	286:291	arg1	formula					293:299	commercial infant formula	275:299	commercial infant formula	275:299	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	1	10	theme	abundant	134:141	arg1	present					182:188	present	182:188	present	182:188	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	1	10	theme	abundant	134:141	arg1	oligosaccharides					143:158	the most abundant oligosaccharides	125:158	the most abundant oligosaccharides that are endogenously present in human breast milk	125:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	5	11	theme	non-digestible	909:922	arg1	oligosaccharide					924:938	a non-digestible oligosaccharide	907:938	a non-digestible oligosaccharide used in infant formula	907:961	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	3	12	theme	LNT	499:501	arg1	evaluation					485:494	the safety evaluation	474:494	the safety evaluation of LNT	474:501	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	2	13	theme	commercial	275:284	arg1	formula					293:299	commercial infant formula	275:299	commercial infant formula	275:299	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	3	14	theme	safety	478:483	arg1	evaluation					485:494	the safety evaluation	474:494	the safety evaluation of LNT	474:501	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	8	15	located	found	1393:1397	arg2	those					1387:1391	those	1387:1391	those	1387:1391	These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.					
30244042	8	15	located	found	1393:1397	arg1	milk					1425:1428	human breast milk	1412:1428	human breast milk	1412:1428	These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.					
30244042	8	16	theme	safe	1303:1306	arg1	use					1308:1310	the safe use	1299:1310	the safe use of LNT in infant formula and as a food ingredient	1299:1360	These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.					
30244042	7	17	theme	compound-related	1109:1124	arg1	effects					1134:1140	no compound-related adverse effects	1106:1140	no compound-related adverse effects	1106:1140	There were no compound-related adverse effects in the 90-day study; therefore, 4000 mg/kg bw/day (the highest feasible dose) was established as the no-observed-adverse-effect-level.					
30244042	3	18	theme	gavage	554:559	arg1	study					570:574	a subchronic oral gavage toxicity study	536:574	a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats)	536:608	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	18	theme	gavage	554:559	arg1	part					466:469	part	466:469	part of the safety evaluation of LNT	466:501	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	18	theme	gavage	554:559	arg1	tests					526:530	in vitro genotoxicity tests	504:530	in vitro genotoxicity tests	504:530	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	0	19	theme	safety	12:17	arg1	evaluation					19:28	Preclinical safety evaluation	0:28	Preclinical safety evaluation of the human-identical milk oligosaccharide	0:72	Preclinical safety evaluation of the human-identical milk oligosaccharide lacto-N-tetraose.					
30244042	4	20	theme	treatment-free	795:808	arg1	period					810:815	a 4-week treatment-free period	786:815	a 4-week treatment-free period	786:815	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	8	21	from	use	1308:1310	arg1	formula					1329:1335	infant formula	1322:1335	infant formula	1322:1335	These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.					
30244042	3	22	theme	neonatal	580:587	arg1	rats					604:607	neonatal Sprague-Dawley rats	580:607	neonatal Sprague-Dawley rats	580:607	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	1	23	theme	human	193:197	arg1	milk					206:209	human breast milk	193:209	human breast milk	193:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	0	24	theme	Preclinical	0:10	arg1	evaluation					19:28	Preclinical safety evaluation	0:28	Preclinical safety evaluation of the human-identical milk oligosaccharide	0:72	Preclinical safety evaluation of the human-identical milk oligosaccharide lacto-N-tetraose.					
30244042	4	25	theme	4-week	788:793	arg1	period					810:815	a 4-week treatment-free period	786:815	a 4-week treatment-free period	786:815	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	3	26	theme	Sprague-Dawley	589:602	arg1	rats					604:607	neonatal Sprague-Dawley rats	580:607	neonatal Sprague-Dawley rats	580:607	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	1	27	theme	breast	199:204	arg1	milk					206:209	human breast milk	193:209	human breast milk	193:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	4	28	theme	bw	732:733	arg1	/day					735:738	0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day	690:738	/day	735:738	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	4	29	theme	body	719:722	arg1	/day					735:738	0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day	690:738	/day	735:738	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	3	30	theme	oral	549:552	arg1	gavage					554:559	a subchronic oral gavage	536:559	a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats)	536:608	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	7	31	theme	feasible	1205:1212	arg1	dose					1214:1217	the highest feasible dose	1193:1217	the highest feasible dose	1193:1217	There were no compound-related adverse effects in the 90-day study; therefore, 4000 mg/kg bw/day (the highest feasible dose) was established as the no-observed-adverse-effect-level.					
30244042	7	31	theme	feasible	1205:1212	arg1	4000 mg/kg bw/day					1174:1190	4000 mg/kg bw/day	1174:1190	4000 mg/kg bw/day (the highest feasible dose)	1174:1218	There were no compound-related adverse effects in the 90-day study; therefore, 4000 mg/kg bw/day (the highest feasible dose) was established as the no-observed-adverse-effect-level.					
30244042	2	32	theme	counterparts	449:460	arg1	versions					411:418	structurally identical versions	388:418	structurally identical versions of their naturally occurring counterparts	388:460	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	0	33	theme	human-identical	37:51	arg1	oligosaccharide					58:72	the human-identical milk oligosaccharide	33:72	the human-identical milk oligosaccharide	33:72	Preclinical safety evaluation of the human-identical milk oligosaccharide lacto-N-tetraose.					
30244042	5	34	theme	fructooligosaccharides	876:897	arg1	powder					899:904	fructooligosaccharides powder	876:904	fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula)	876:962	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	1	35	attach	present	182:188	arg2	present					182:188	present	182:188	present	182:188	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	1	35	attach	present	182:188	arg2	oligosaccharides					143:158	the most abundant oligosaccharides	125:158	the most abundant oligosaccharides that are endogenously present in human breast milk	125:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	1	35	attach	present	182:188	arg1	milk					206:209	human breast milk	193:209	human breast milk	193:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	2	36	theme	human	243:247	arg1	milk					256:259	human breast milk	243:259	human breast milk	243:259	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	2	37	theme	identical	401:409	arg1	versions					411:418	structurally identical versions	388:418	structurally identical versions of their naturally occurring counterparts	388:460	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	2	38	theme	occurring	439:447	arg1	counterparts					449:460	their naturally occurring counterparts	423:460	their naturally occurring counterparts	423:460	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	4	39	theme	0	690:690	arg1	/day					735:738	0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day	690:738	/day	735:738	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	7	40	theme	adverse	1126:1132	arg1	effects					1134:1140	no compound-related adverse effects	1106:1140	no compound-related adverse effects	1106:1140	There were no compound-related adverse effects in the 90-day study; therefore, 4000 mg/kg bw/day (the highest feasible dose) was established as the no-observed-adverse-effect-level.					
30244042	8	41	theme	breast	1418:1423	arg1	milk					1425:1428	human breast milk	1412:1428	human breast milk	1412:1428	These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.					
30244042	6	42	from	tests	1088:1092	arg1	non-genotoxic					1058:1070	non-genotoxic	1058:1070	non-genotoxic	1058:1070	LNT was non-genotoxic in the in vitro tests.					
30244042	3	43	theme	evaluation	485:494	arg1	study					570:574	a subchronic oral gavage toxicity study	536:574	a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats)	536:608	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	43	theme	evaluation	485:494	arg1	part					466:469	part	466:469	part of the safety evaluation of LNT	466:501	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	43	theme	evaluation	485:494	arg1	tests					526:530	in vitro genotoxicity tests	504:530	in vitro genotoxicity tests	504:530	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	0	44	theme	oligosaccharide	58:72	arg1	evaluation					19:28	Preclinical safety evaluation	0:28	Preclinical safety evaluation of the human-identical milk oligosaccharide	0:72	Preclinical safety evaluation of the human-identical milk oligosaccharide lacto-N-tetraose.					
30244042	5	45	theme	comprised	833:841	arg1	group					861:865	An identically comprised reference control group	818:865	An identically comprised reference control group	818:865	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	5	46	dep	powder	899:904	arg1	oligosaccharide					924:938	a non-digestible oligosaccharide	907:938	a non-digestible oligosaccharide used in infant formula	907:961	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	8	47	theme	human	1412:1416	arg1	milk					1425:1428	human breast milk	1412:1428	human breast milk	1412:1428	These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.					
30244042	5	48	theme	infant	948:953	arg1	formula					955:961	infant formula	948:961	infant formula	948:961	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	6	49	theme	in	1079:1080	arg1	tests					1088:1092	the in vitro tests	1075:1092	the in vitro tests	1075:1092	LNT was non-genotoxic in the in vitro tests.					
30244042	1	50	dep	Lacto-N-tetraose	92:107	arg1	LNT					110:112	LNT	110:112	LNT	110:112	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	0	51	theme	milk	53:56	arg1	oligosaccharide					58:72	the human-identical milk oligosaccharide	33:72	the human-identical milk oligosaccharide	33:72	Preclinical safety evaluation of the human-identical milk oligosaccharide lacto-N-tetraose.					
30244042	1	52	from	milk	206:209	arg1	present					182:188	present	182:188	present	182:188	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	1	52	from	milk	206:209	arg1	oligosaccharides					143:158	the most abundant oligosaccharides	125:158	the most abundant oligosaccharides that are endogenously present in human breast milk	125:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	3	53	theme	genotoxicity	513:524	arg1	study					570:574	a subchronic oral gavage toxicity study	536:574	a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats)	536:608	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	53	theme	genotoxicity	513:524	arg1	part					466:469	part	466:469	part of the safety evaluation of LNT	466:501	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	53	theme	genotoxicity	513:524	arg1	tests					526:530	in vitro genotoxicity tests	504:530	in vitro genotoxicity tests	504:530	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	4	54	dep	administered	659:670	arg1	followed					774:781	followed	774:781	followed by a 4-week treatment-free period	774:815	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	4	55	theme	dose	675:678	arg1	levels					680:685	dose levels	675:685	dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day	675:738	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	2	56	theme	milk	342:345	arg1	oligosaccharides					347:362	human-identical milk oligosaccharides	326:362	human-identical milk oligosaccharides	326:362	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	8	57	theme	food	1346:1349	arg1	ingredient					1351:1360	a food ingredient	1344:1360	a food ingredient	1344:1360	These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.					
30244042	8	58	theme	infant	1322:1327	arg1	formula					1329:1335	infant formula	1322:1335	infant formula	1322:1335	These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.					
30244042	5	59	theme	high-dose	1029:1037	arg1	group					1043:1047	the high-dose LNT group	1025:1047	the high-dose LNT group	1025:1047	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	4	60	theme	/day	735:738	arg1	levels					680:685	dose levels	675:685	dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day	675:738	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	1	61	located	present	182:188	arg2	present					182:188	present	182:188	present	182:188	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	1	61	located	present	182:188	arg2	oligosaccharides					143:158	the most abundant oligosaccharides	125:158	the most abundant oligosaccharides that are endogenously present in human breast milk	125:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	1	61	located	present	182:188	arg1	milk					206:209	human breast milk	193:209	human breast milk	193:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	2	62	theme	human-identical	326:340	arg1	oligosaccharides					347:362	human-identical milk oligosaccharides	326:362	human-identical milk oligosaccharides	326:362	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	8	63	theme	LNT	1315:1317	arg1	use					1308:1310	the safe use	1299:1310	the safe use of LNT in infant formula and as a food ingredient	1299:1360	These results support the safe use of LNT in infant formula and as a food ingredient, at levels not exceeding those found naturally in human breast milk.					
30244042	4	64	theme	4000 mg/kg	708:717	arg1	/day					735:738	0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day	690:738	/day	735:738	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	5	65	theme	reference	843:851	arg1	group					861:865	An identically comprised reference control group	818:865	An identically comprised reference control group	818:865	An identically comprised reference control group received fructooligosaccharides powder (a non-digestible oligosaccharide used in infant formula) at 4000 mg/kg bw/day, to allow for direct comparison against the high-dose LNT group.					
30244042	2	66	theme	milk	256:259	arg1	composition					228:238	the composition	224:238	the composition of human breast milk	224:259	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	6	67	from	non-genotoxic	1058:1070	arg1	tests					1088:1092	the in vitro tests	1075:1092	the in vitro tests	1075:1092	LNT was non-genotoxic in the in vitro tests.					
30244042	1	68	theme	oligosaccharides	143:158	arg1	present					182:188	present	182:188	present	182:188	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	1	68	theme	oligosaccharides	143:158	arg1	oligosaccharides					143:158	the most abundant oligosaccharides	125:158	the most abundant oligosaccharides that are endogenously present in human breast milk	125:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	1	68	theme	oligosaccharides	143:158	arg1	one					118:120	one	118:120	one	118:120	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	3	69	theme	in	504:505	arg1	study					570:574	a subchronic oral gavage toxicity study	536:574	a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats)	536:608	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	69	theme	in	504:505	arg1	part					466:469	part	466:469	part of the safety evaluation of LNT	466:501	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	3	69	theme	in	504:505	arg1	tests					526:530	in vitro genotoxicity tests	504:530	in vitro genotoxicity tests	504:530	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	4	70	theme	subchronic	633:642	arg1	study					644:648	the subchronic study	629:648	the subchronic study	629:648	In the subchronic study, LNT was administered at dose levels of 0, 1,000, 2500 or 4000 mg/kg body weight (bw)/day, once daily for at least 90 days, followed by a 4-week treatment-free period.					
30244042	7	71	theme	highest	1197:1203	arg1	dose					1214:1217	the highest feasible dose	1193:1217	the highest feasible dose	1193:1217	There were no compound-related adverse effects in the 90-day study; therefore, 4000 mg/kg bw/day (the highest feasible dose) was established as the no-observed-adverse-effect-level.					
30244042	7	71	theme	highest	1197:1203	arg1	4000 mg/kg bw/day					1174:1190	4000 mg/kg bw/day	1174:1190	4000 mg/kg bw/day (the highest feasible dose)	1174:1218	There were no compound-related adverse effects in the 90-day study; therefore, 4000 mg/kg bw/day (the highest feasible dose) was established as the no-observed-adverse-effect-level.					
30244042	2	72	theme	breast	249:254	arg1	milk					256:259	human breast milk	243:259	human breast milk	243:259	To simulate the composition of human breast milk more closely, commercial infant formula can be supplemented with human-identical milk oligosaccharides, which are manufactured structurally identical versions of their naturally occurring counterparts.					
30244042	1	73	from	present	182:188	arg1	milk					206:209	human breast milk	193:209	human breast milk	193:209	Lacto-N-tetraose (LNT) is one of the most abundant oligosaccharides that are endogenously present in human breast milk.					
30244042	3	74	theme	subchronic	538:547	arg1	gavage					554:559	a subchronic oral gavage	536:559	a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats)	536:608	As part of the safety evaluation of LNT, in vitro genotoxicity tests and a subchronic oral gavage toxicity study (in neonatal Sprague-Dawley rats) were conducted.					
30244042	7	75	theme	90-day	1149:1154	arg1	study					1156:1160	the 90-day study	1145:1160	the 90-day study	1145:1160	There were no compound-related adverse effects in the 90-day study; therefore, 4000 mg/kg bw/day (the highest feasible dose) was established as the no-observed-adverse-effect-level.					
31060346	5	0	theme	matrix	1099:1104	arg1	structure					1082:1090	the three-dimensional structure	1060:1090	the three-dimensional structure of the matrix	1060:1104	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	5	0	theme	matrix	1099:1104	arg1	arrangement					958:968	the arrangement	954:968	the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix	954:1054	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	2	1	theme	several	392:398	arg1	bone					400:403	several bone and joint disease	392:421	bone	400:403	Due to its excellent bio-mimicry property, collagen is used for the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia.					
31060346	8	2	theme	bone	1699:1702	arg1	engineering					1711:1721	bone tissue engineering	1699:1721	bone tissue engineering	1699:1721	From that, it was hypothesized that the morphology of the CCHCs 3D-matrix resembles trabecular bone, which enhances bone growth, limits bone resorption, and could be a novel biomaterial for bone tissue engineering.					
31060346	5	3	dep	three-dimensional	1026:1042	arg1	3D					1045:1046	3D	1045:1046	3D	1045:1046	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	4	4	theme	bone	841:844	arg1	marrow					846:851	bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages	841:915	marrow	846:851	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	4	5	theme	mesenchymal	853:863	arg1	cells					870:874	mesenchymal stem cells	853:874	mesenchymal stem cells	853:874	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	0	6	theme	Osteoblast	113:122	arg1	Cues					90:93	RANKL-Mediated Paracrine Cues	65:93	RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis	65:203	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	4	7	theme	bone-like	765:773	arg1	structure					778:786	a natural bone-like 3D structure	755:786	a natural bone-like 3D structure	755:786	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	4	8	theme	stem	865:868	arg1	cells					870:874	mesenchymal stem cells	853:874	mesenchymal stem cells	853:874	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	2	9	theme	bio-mimicry	332:342	arg1	property					344:351	its excellent bio-mimicry property	318:351	its excellent bio-mimicry property	318:351	Due to its excellent bio-mimicry property, collagen is used for the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia.					
31060346	4	10	from	effect	808:813	arg1	homeostasis					823:833	bone homeostasis	818:833	bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages	818:915	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	0	11	theme	Differentiated	98:111	arg1	Osteoblast					113:122	Differentiated Osteoblast and Mesenchymal Stem Cells	98:149	Osteoblast	113:122	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	4	12	theme	perceptions	641:651	arg1	view					623:626	view	623:626	view of the above perceptions	623:651	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	3	13	theme	bone	565:568	arg1	materials					570:578	other bone materials	559:578	other bone materials	559:578	In bone, the biological action of collagen is highly influenced by the interactions of other bone materials such as glycosaminoglycan and minerals.					
31060346	2	14	theme	excellent	322:330	arg1	property					344:351	its excellent bio-mimicry property	318:351	its excellent bio-mimicry property	318:351	Due to its excellent bio-mimicry property, collagen is used for the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia.					
31060346	0	15	theme	Mesenchymal	128:138	arg1	Cells					145:149	Differentiated Osteoblast and Mesenchymal Stem Cells	98:149	Cells	145:149	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	5	16	theme	three-dimensional	1064:1080	arg1	structure					1082:1090	the three-dimensional structure	1060:1090	the three-dimensional structure of the matrix	1060:1104	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	4	17	theme	bone	818:821	arg1	homeostasis					823:833	bone homeostasis	818:833	bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages	818:915	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	3	18	theme	biological	485:494	arg1	action					496:501	the biological action	481:501	the biological action of collagen	481:513	In bone, the biological action of collagen is highly influenced by the interactions of other bone materials such as glycosaminoglycan and minerals.					
31060346	4	19	theme	natural	757:763	arg1	structure					778:786	a natural bone-like 3D structure	755:786	a natural bone-like 3D structure	755:786	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	1	20	theme	Recent	206:211	arg1	studies					213:219	Recent studies	206:219	Recent studies	206:219	Recent studies have identified the regulatory mechanism of collagen in bone ossification and resorption.					
31060346	7	21	theme	CCHCs	1492:1496	arg1	3D-matrix					1498:1506	the CCHCs 3D-matrix	1488:1506	the CCHCs 3D-matrix	1488:1506	Besides, osteogenic progenitor's paracrine cues for osteoclastogenesis showed that the differentiated osteoblast secreted higher levels of RANKL to support osteoclastogenesis, and the effect was downregulated by the CCHCs 3D-matrix.					
31060346	2	22	used	used	366:369	arg2	collagen					354:361	collagen	354:361	collagen	354:361	Due to its excellent bio-mimicry property, collagen is used for the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia.					
31060346	6	23	theme	osteoclastogenic	1155:1170	arg1	activity					1172:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity	1107:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices	1107:1194	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices were identified using differentiated osteoblasts and osteoclasts, respectively.					
31060346	5	24	from	arrangement	958:968	arg1	-matrix					1048:1054	the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix	991:1054	the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix	991:1054	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	0	25	theme	3D	18:19	arg1	Matrix					21:26	Chitosan-Collagen 3D Matrix	0:26	Chitosan-Collagen 3D Matrix	0:26	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	8	26	theme	CCHCs	1567:1571	arg1	3D-matrix					1573:1581	the CCHCs 3D-matrix	1563:1581	the CCHCs 3D-matrix	1563:1581	From that, it was hypothesized that the morphology of the CCHCs 3D-matrix resembles trabecular bone, which enhances bone growth, limits bone resorption, and could be a novel biomaterial for bone tissue engineering.					
31060346	6	27	theme	exploitive	1144:1153	arg1	activity					1172:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity	1107:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices	1107:1194	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices were identified using differentiated osteoblasts and osteoclasts, respectively.					
31060346	2	28	theme	disease	415:421	arg1	treatment					379:387	the treatment	375:387	the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia	375:469	Due to its excellent bio-mimicry property, collagen is used for the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia.					
31060346	0	29	theme	Chitosan-Collagen	0:16	arg1	Matrix					21:26	Chitosan-Collagen 3D Matrix	0:26	Chitosan-Collagen 3D Matrix	0:26	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	8	30	theme	trabecular	1593:1602	arg1	bone					1604:1607	trabecular bone	1593:1607	trabecular bone	1593:1607	From that, it was hypothesized that the morphology of the CCHCs 3D-matrix resembles trabecular bone, which enhances bone growth, limits bone resorption, and could be a novel biomaterial for bone tissue engineering.					
31060346	2	31	theme	joint	409:413	arg1	disease					415:421	several bone and joint disease	392:421	disease	415:421	Due to its excellent bio-mimicry property, collagen is used for the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia.					
31060346	0	32	theme	Cells	145:149	arg1	Cues					90:93	RANKL-Mediated Paracrine Cues	65:93	RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis	65:203	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	3	33	theme	materials	570:578	arg1	interactions					543:554	the interactions	539:554	the interactions of other bone materials such as glycosaminoglycan and minerals	539:617	In bone, the biological action of collagen is highly influenced by the interactions of other bone materials such as glycosaminoglycan and minerals.					
31060346	3	33	theme	materials	570:578	arg1	minerals					610:617	minerals	610:617	minerals	610:617	In bone, the biological action of collagen is highly influenced by the interactions of other bone materials such as glycosaminoglycan and minerals.					
31060346	3	33	theme	materials	570:578	arg1	glycosaminoglycan					588:604	glycosaminoglycan	588:604	glycosaminoglycan	588:604	In bone, the biological action of collagen is highly influenced by the interactions of other bone materials such as glycosaminoglycan and minerals.					
31060346	5	34	theme	micro-CT	930:937	arg1	data					939:942	The XRD and micro-CT data	918:942	data	939:942	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	5	35	from	structure	1082:1090	arg1	-matrix					1048:1054	the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix	991:1054	the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix	991:1054	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	6	36	theme	3D-matrices	1184:1194	arg1	activity					1172:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity	1107:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices	1107:1194	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices were identified using differentiated osteoblasts and osteoclasts, respectively.					
31060346	0	37	theme	Stem	140:143	arg1	Cells					145:149	Differentiated Osteoblast and Mesenchymal Stem Cells	98:149	Cells	145:149	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	4	38	attach	crosslinked	667:677	arg3	view					623:626	view	623:626	view of the above perceptions	623:651	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	6	39	theme	stimulatory	1111:1121	arg1	activity					1172:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity	1107:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices	1107:1194	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices were identified using differentiated osteoblasts and osteoclasts, respectively.					
31060346	1	40	theme	regulatory	241:250	arg1	mechanism					252:260	the regulatory mechanism	237:260	the regulatory mechanism of collagen in bone ossification and resorption	237:308	Recent studies have identified the regulatory mechanism of collagen in bone ossification and resorption.					
31060346	0	41	theme	Bone	155:158	arg1	Marrow					160:165	Bone Marrow Macrophage-Derived	155:184	Bone Marrow Macrophage-Derived Osteoclastogenesis	155:203	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	0	42	theme	Trabecular	35:44	arg1	Bone					46:49	Trabecular Bone	35:49	Trabecular Bone	35:49	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	4	43	theme	marrow	898:903	arg1	macrophages					905:915	bone marrow macrophages	893:915	bone marrow macrophages	893:915	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	7	44	theme	differentiated	1363:1376	arg1	osteoblast					1378:1387	the differentiated osteoblast	1359:1387	the differentiated osteoblast	1359:1387	Besides, osteogenic progenitor's paracrine cues for osteoclastogenesis showed that the differentiated osteoblast secreted higher levels of RANKL to support osteoclastogenesis, and the effect was downregulated by the CCHCs 3D-matrix.					
31060346	4	45	theme	above	635:639	arg1	perceptions					641:651	the above perceptions	631:651	the above perceptions	631:651	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	1	46	from	mechanism	252:260	arg1	resorption					299:308	resorption	299:308	resorption	299:308	Recent studies have identified the regulatory mechanism of collagen in bone ossification and resorption.					
31060346	1	46	from	mechanism	252:260	arg1	ossification					282:293	bone ossification	277:293	bone ossification	277:293	Recent studies have identified the regulatory mechanism of collagen in bone ossification and resorption.					
31060346	8	47	theme	bone	1625:1628	arg1	growth					1630:1635	bone growth	1625:1635	bone growth	1625:1635	From that, it was hypothesized that the morphology of the CCHCs 3D-matrix resembles trabecular bone, which enhances bone growth, limits bone resorption, and could be a novel biomaterial for bone tissue engineering.					
31060346	4	48	dep	marrow	846:851	arg1	cells					870:874	mesenchymal stem cells	853:874	mesenchymal stem cells	853:874	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	0	49	theme	Macrophage-Derived	167:184	arg1	Marrow					160:165	Bone Marrow Macrophage-Derived	155:184	Bone Marrow Macrophage-Derived Osteoclastogenesis	155:203	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	7	50	theme	RANKL	1415:1419	arg1	levels					1405:1410	higher levels	1398:1410	higher levels of RANKL	1398:1419	Besides, osteogenic progenitor's paracrine cues for osteoclastogenesis showed that the differentiated osteoblast secreted higher levels of RANKL to support osteoclastogenesis, and the effect was downregulated by the CCHCs 3D-matrix.					
31060346	8	51	theme	3D-matrix	1573:1581	arg1	morphology					1549:1558	the morphology	1545:1558	the morphology of the CCHCs 3D-matrix	1545:1581	From that, it was hypothesized that the morphology of the CCHCs 3D-matrix resembles trabecular bone, which enhances bone growth, limits bone resorption, and could be a novel biomaterial for bone tissue engineering.					
31060346	3	52	theme	collagen	506:513	arg1	action					496:501	the biological action	481:501	the biological action of collagen	481:513	In bone, the biological action of collagen is highly influenced by the interactions of other bone materials such as glycosaminoglycan and minerals.					
31060346	0	53	theme	Marrow	160:165	arg1	Osteoclastogenesis					186:203	Bone Marrow Macrophage-Derived Osteoclastogenesis	155:203	Bone Marrow Macrophage-Derived Osteoclastogenesis	155:203	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	6	54	theme	osteoblastogenic	1123:1138	arg1	activity					1172:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity	1107:1179	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices	1107:1194	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices were identified using differentiated osteoblasts and osteoclasts, respectively.					
31060346	7	55	theme	paracrine	1309:1317	arg1	cues					1319:1322	osteogenic progenitor's paracrine cues	1285:1322	osteogenic progenitor's paracrine cues for osteoclastogenesis	1285:1345	Besides, osteogenic progenitor's paracrine cues for osteoclastogenesis showed that the differentiated osteoblast secreted higher levels of RANKL to support osteoclastogenesis, and the effect was downregulated by the CCHCs 3D-matrix.					
31060346	8	56	theme	novel	1677:1681	arg1	biomaterial					1683:1693	a novel biomaterial	1675:1693	a novel biomaterial for bone tissue engineering	1675:1721	From that, it was hypothesized that the morphology of the CCHCs 3D-matrix resembles trabecular bone, which enhances bone growth, limits bone resorption, and could be a novel biomaterial for bone tissue engineering.					
31060346	7	57	theme	higher	1398:1403	arg1	levels					1405:1410	higher levels	1398:1410	higher levels of RANKL	1398:1419	Besides, osteogenic progenitor's paracrine cues for osteoclastogenesis showed that the differentiated osteoblast secreted higher levels of RANKL to support osteoclastogenesis, and the effect was downregulated by the CCHCs 3D-matrix.					
31060346	6	58	theme	differentiated	1218:1231	arg1	osteoblasts					1233:1243	osteoblasts	1233:1243	osteoblasts	1233:1243	The stimulatory osteoblastogenic and exploitive osteoclastogenic activity of 3D-matrices were identified using differentiated osteoblasts and osteoclasts, respectively.					
31060346	4	59	theme	3D	775:776	arg1	structure					778:786	a natural bone-like 3D structure	755:786	a natural bone-like 3D structure	755:786	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	7	60	theme	osteogenic	1285:1294	arg1	cues					1319:1322	osteogenic progenitor's paracrine cues	1285:1322	osteogenic progenitor's paracrine cues for osteoclastogenesis	1285:1345	Besides, osteogenic progenitor's paracrine cues for osteoclastogenesis showed that the differentiated osteoblast secreted higher levels of RANKL to support osteoclastogenesis, and the effect was downregulated by the CCHCs 3D-matrix.					
31060346	5	61	theme	XRD	922:924	arg1	data					939:942	The XRD and micro-CT data	918:942	data	939:942	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	1	62	theme	collagen	265:272	arg1	mechanism					252:260	the regulatory mechanism	237:260	the regulatory mechanism of collagen in bone ossification and resorption	237:308	Recent studies have identified the regulatory mechanism of collagen in bone ossification and resorption.					
31060346	5	63	theme	three-dimensional	1026:1042	arg1	-matrix					1048:1054	the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix	991:1054	the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix	991:1054	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	3	64	theme	other	559:563	arg1	materials					570:578	other bone materials	559:578	other bone materials	559:578	In bone, the biological action of collagen is highly influenced by the interactions of other bone materials such as glycosaminoglycan and minerals.					
31060346	4	65	theme	chondroitin	718:728	arg1	Cs					739:740	Cs	739:740	Cs	739:740	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	4	65	theme	chondroitin	718:728	arg1	sulfate					730:736	chondroitin sulfate	718:736	chondroitin sulfate (Cs)	718:741	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	0	66	theme	Paracrine	80:88	arg1	Cues					90:93	RANKL-Mediated Paracrine Cues	65:93	RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis	65:203	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	4	67	theme	bone	893:896	arg1	macrophages					905:915	bone marrow macrophages	893:915	bone marrow macrophages	893:915	In view of the above perceptions, collagen was crosslinked with chitosan, hydroxyapatite (H), and chondroitin sulfate (Cs), to produce a natural bone-like 3D structure and to evaluate its effect on bone homeostasis using bone marrow mesenchymal stem cells, osteoblast, and bone marrow macrophages.					
31060346	5	68	theme	H	973:973	arg1	crystallites					975:986	H crystallites	973:986	H crystallites	973:986	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	1	69	theme	bone	277:280	arg1	ossification					282:293	bone ossification	277:293	bone ossification	277:293	Recent studies have identified the regulatory mechanism of collagen in bone ossification and resorption.					
31060346	0	70	theme	RANKL-Mediated	65:78	arg1	Cues					90:93	RANKL-Mediated Paracrine Cues	65:93	RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis	65:203	Chitosan-Collagen 3D Matrix Mimics Trabecular Bone and Regulates RANKL-Mediated Paracrine Cues of Differentiated Osteoblast and Mesenchymal Stem Cells for Bone Marrow Macrophage-Derived Osteoclastogenesis.					
31060346	5	71	theme	crystallites	975:986	arg1	structure					1082:1090	the three-dimensional structure	1060:1090	the three-dimensional structure of the matrix	1060:1104	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	5	71	theme	crystallites	975:986	arg1	arrangement					958:968	the arrangement	954:968	the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix	954:1054	The XRD and micro-CT data confirmed the arrangement of H crystallites in the chitosan-collagen-H-Cs (CCHCs) three-dimensional (3D)-matrix and the three-dimensional structure of the matrix.					
31060346	2	72	theme	bone	400:403	arg1	treatment					379:387	the treatment	375:387	the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia	375:469	Due to its excellent bio-mimicry property, collagen is used for the treatment of several bone and joint disease such as arthritis, osteoporosis, and osteopenia.					
31060346	8	73	theme	tissue	1704:1709	arg1	engineering					1711:1721	bone tissue engineering	1699:1721	bone tissue engineering	1699:1721	From that, it was hypothesized that the morphology of the CCHCs 3D-matrix resembles trabecular bone, which enhances bone growth, limits bone resorption, and could be a novel biomaterial for bone tissue engineering.					
31060346	8	74	theme	bone	1645:1648	arg1	resorption					1650:1659	bone resorption	1645:1659	bone resorption	1645:1659	From that, it was hypothesized that the morphology of the CCHCs 3D-matrix resembles trabecular bone, which enhances bone growth, limits bone resorption, and could be a novel biomaterial for bone tissue engineering.					
29743543	0	0	theme	cell	92:95	arg1	potential					64:72	metastatic potential	53:72	metastatic potential of mouse hepatoma cell	53:95	MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3.					
29743543	7	1	theme	Mgat3	1426:1430	arg1	targeting					1389:1397	the targeting	1385:1397	the targeting of the glycosyltransferase Mgat3	1385:1430	Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3.					
29743543	1	2	theme	aberrant	150:157	arg1	glycosylation					159:171	aberrant glycosylation	150:171	aberrant glycosylation	150:171	MicroRNAs (miRNAs) and aberrant glycosylation both play important roles in tumor metastasis.					
29743543	2	3	from	metastasis	282:291	arg1	N-glycosylation					258:272	N-glycosylation	258:272	N-glycosylation	258:272	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	0	4	theme	hepatoma	83:90	arg1	cell					92:95	mouse hepatoma cell	77:95	mouse hepatoma cell	77:95	MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3.					
29743543	2	5	theme	cells	333:337	arg1	role					239:242	the role	235:242	the role of miR-23a in N-glycosylation	235:272	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	2	5	theme	cells	333:337	arg1	metastasis					282:291	the metastasis	278:291	the metastasis of mouse hepatocellular carcinoma (HCC) cells	278:337	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	5	6	theme	gene	979:982	arg1	assays					984:989	dual-luciferase reporter gene assays	954:989	dual-luciferase reporter gene assays	954:989	Bioinformatics analysis indicated that Mgat3 may be a target of miR-23a, and this hypothesis was verified by dual-luciferase reporter gene assays.					
29743543	6	7	theme	gene	1083:1086	arg1	promoter					1088:1095	the miR-23a gene promoter	1071:1095	the miR-23a gene promoter	1071:1095	Furthermore, we found that the transcription factor Runx2 can directly bind to the miR-23a gene promoter and promote its expression, as shown in dual-luciferase reporter gene assays and ChIP assays.					
29743543	6	8	theme	reporter	1153:1160	arg1	assays					1167:1172	dual-luciferase reporter gene assays	1137:1172	dual-luciferase reporter gene assays	1137:1172	Furthermore, we found that the transcription factor Runx2 can directly bind to the miR-23a gene promoter and promote its expression, as shown in dual-luciferase reporter gene assays and ChIP assays.					
29743543	3	9	theme	Hepa1-6	550:556	arg1	cells					558:562	Hepa1-6 cells	550:562	Hepa1-6 cells (with no lymphatic metastasis potential)	550:603	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	3	10	theme	metastasis	711:720	arg1	potential					722:730	metastasis potential	711:730	metastasis potential	711:730	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	4	11	theme	key	744:746	arg1	glycosyltransferase					748:766	a key glycosyltransferase	742:766	a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure	742:842	Mgat3 is a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure.					
29743543	4	11	theme	key	744:746	arg1	Mgat3					733:737	Mgat3	733:737	Mgat3	733:737	Mgat3 is a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure.					
29743543	4	12	from	glycosyltransferase	748:766	arg1	synthesis					775:783	the synthesis	771:783	the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure	771:842	Mgat3 is a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure.					
29743543	3	13	theme	Hca-P	492:496	arg1	cells					498:502	Hca-P cells	492:502	Hca-P cells	492:502	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	3	14	theme	expression	471:480	arg1	levels					482:487	higher miR-23a expression levels	456:487	higher miR-23a expression levels	456:487	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	8	15	theme	aberrant	1528:1535	arg1	glycosylation					1537:1549	aberrant glycosylation	1528:1549	aberrant glycosylation	1528:1549	These findings may provide insight into the relationship between abnormal miRNA expression and aberrant glycosylation during tumor lymphatic metastasis.					
29743543	4	16	theme	structure	834:842	arg1	synthesis					775:783	the synthesis	771:783	the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure	771:842	Mgat3 is a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure.					
29743543	3	17	theme	higher	456:461	arg1	levels					482:487	higher miR-23a expression levels	456:487	higher miR-23a expression levels	456:487	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	3	18	theme	lymphatic	510:518	arg1	potential					531:539	lymphatic metastasis potential	510:539	lymphatic metastasis potential	510:539	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	7	19	theme	N-glycan	1331:1338	arg1	chains					1340:1345	N-glycan chains	1331:1345	N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3	1331:1430	Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3.					
29743543	8	20	theme	tumor	1558:1562	arg1	metastasis					1574:1583	tumor lymphatic metastasis	1558:1583	tumor lymphatic metastasis	1558:1583	These findings may provide insight into the relationship between abnormal miRNA expression and aberrant glycosylation during tumor lymphatic metastasis.					
29743543	4	21	theme	N-glycan	825:832	arg1	structure					834:842	the bisecting (β1,4GlcNAc branching) N-glycan structure	788:842	the bisecting (β1,4GlcNAc branching) N-glycan structure	788:842	Mgat3 is a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure.					
29743543	1	22	dep	MicroRNAs	127:135	arg1	both					173:176	both	173:176	both	173:176	MicroRNAs (miRNAs) and aberrant glycosylation both play important roles in tumor metastasis.					
29743543	3	23	theme	Western	425:431	arg1	analyses					438:445	Western blot analyses	425:445	Western blot analyses	425:445	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	0	24	theme	transcriptional	8:22	arg1	MiR-23a					0:6	MiR-23a transcriptional	0:22	MiR-23a transcriptional activated by Runx2	0:41	MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3.					
29743543	6	25	theme	dual-luciferase	1137:1151	arg1	assays					1167:1172	dual-luciferase reporter gene assays	1137:1172	dual-luciferase reporter gene assays	1137:1172	Furthermore, we found that the transcription factor Runx2 can directly bind to the miR-23a gene promoter and promote its expression, as shown in dual-luciferase reporter gene assays and ChIP assays.					
29743543	7	26	theme	mouse	1284:1288	arg1	HCC					1290:1292	mouse HCC	1284:1292	mouse HCC	1284:1292	Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3.					
29743543	2	27	theme	hepatocellular	302:315	arg1	HCC					328:330	HCC	328:330	HCC	328:330	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	2	27	theme	hepatocellular	302:315	arg1	carcinoma					317:325	mouse hepatocellular carcinoma	296:325	mouse hepatocellular carcinoma (HCC) cells	296:337	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	7	28	theme	metastatic	1260:1269	arg1	potential					1271:1279	the metastatic potential	1256:1279	the metastatic potential of mouse HCC	1256:1292	Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3.					
29743543	1	29	theme	important	183:191	arg1	roles					193:197	important roles	183:197	important roles	183:197	MicroRNAs (miRNAs) and aberrant glycosylation both play important roles in tumor metastasis.					
29743543	3	30	theme	array	378:382	arg1	profiles					384:391	The miRNA expression array profiles	357:391	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses	357:445	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	2	31	theme	mouse	296:300	arg1	HCC					328:330	HCC	328:330	HCC	328:330	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	2	31	theme	mouse	296:300	arg1	carcinoma					317:325	mouse hepatocellular carcinoma	296:325	mouse hepatocellular carcinoma (HCC) cells	296:337	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	2	32	from	role	239:242	arg1	N-glycosylation					258:272	N-glycosylation	258:272	N-glycosylation	258:272	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	7	33	theme	branch	1311:1316	arg1	formation					1318:1326	the branch formation	1307:1326	the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3	1307:1430	Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3.					
29743543	4	34	theme	bisecting	792:800	arg1	structure					834:842	the bisecting (β1,4GlcNAc branching) N-glycan structure	788:842	the bisecting (β1,4GlcNAc branching) N-glycan structure	788:842	Mgat3 is a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure.					
29743543	7	35	theme	cell	1364:1367	arg1	surface					1369:1375	the cell surface	1360:1375	the cell surface	1360:1375	Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3.					
29743543	3	36	theme	miRNA	361:365	arg1	profiles					384:391	The miRNA expression array profiles	357:391	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses	357:445	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	5	37	theme	reporter	970:977	arg1	assays					984:989	dual-luciferase reporter gene assays	954:989	dual-luciferase reporter gene assays	954:989	Bioinformatics analysis indicated that Mgat3 may be a target of miR-23a, and this hypothesis was verified by dual-luciferase reporter gene assays.					
29743543	6	38	theme	miR-23a	1075:1081	arg1	promoter					1088:1095	the miR-23a gene promoter	1071:1095	the miR-23a gene promoter	1071:1095	Furthermore, we found that the transcription factor Runx2 can directly bind to the miR-23a gene promoter and promote its expression, as shown in dual-luciferase reporter gene assays and ChIP assays.					
29743543	1	39	theme	tumor	202:206	arg1	metastasis					208:217	tumor metastasis	202:217	tumor metastasis	202:217	MicroRNAs (miRNAs) and aberrant glycosylation both play important roles in tumor metastasis.					
29743543	7	40	theme	HCC	1290:1292	arg1	potential					1271:1279	the metastatic potential	1256:1279	the metastatic potential of mouse HCC	1256:1292	Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3.					
29743543	6	41	theme	factor	1037:1042	arg1	Runx2					1044:1048	the transcription factor Runx2	1019:1048	the transcription factor Runx2	1019:1048	Furthermore, we found that the transcription factor Runx2 can directly bind to the miR-23a gene promoter and promote its expression, as shown in dual-luciferase reporter gene assays and ChIP assays.					
29743543	4	42	theme	β1,4GlcNAc	803:812	arg1	structure					834:842	the bisecting (β1,4GlcNAc branching) N-glycan structure	788:842	the bisecting (β1,4GlcNAc branching) N-glycan structure	788:842	Mgat3 is a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure.					
29743543	3	43	theme	blot	433:436	arg1	analyses					438:445	Western blot analyses	425:445	Western blot analyses	425:445	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	3	44	theme	lymphatic	573:581	arg1	metastasis					583:592	no lymphatic metastasis	570:592	no lymphatic metastasis potential	570:602	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	5	45	theme	Bioinformatics	845:858	arg1	analysis					860:867	Bioinformatics analysis	845:867	Bioinformatics analysis	845:867	Bioinformatics analysis indicated that Mgat3 may be a target of miR-23a, and this hypothesis was verified by dual-luciferase reporter gene assays.					
29743543	6	46	theme	transcription	1023:1035	arg1	Runx2					1044:1048	the transcription factor Runx2	1019:1048	the transcription factor Runx2	1019:1048	Furthermore, we found that the transcription factor Runx2 can directly bind to the miR-23a gene promoter and promote its expression, as shown in dual-luciferase reporter gene assays and ChIP assays.					
29743543	8	47	theme	lymphatic	1564:1572	arg1	metastasis					1574:1583	tumor lymphatic metastasis	1558:1583	tumor lymphatic metastasis	1558:1583	These findings may provide insight into the relationship between abnormal miRNA expression and aberrant glycosylation during tumor lymphatic metastasis.					
29743543	5	48	theme	dual-luciferase	954:968	arg1	assays					984:989	dual-luciferase reporter gene assays	954:989	dual-luciferase reporter gene assays	954:989	Bioinformatics analysis indicated that Mgat3 may be a target of miR-23a, and this hypothesis was verified by dual-luciferase reporter gene assays.					
29743543	0	49	theme	metastatic	53:62	arg1	potential					64:72	metastatic potential	53:72	metastatic potential of mouse hepatoma cell	53:95	MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3.					
29743543	4	50	theme	branching	814:822	arg1	structure					834:842	the bisecting (β1,4GlcNAc branching) N-glycan structure	788:842	the bisecting (β1,4GlcNAc branching) N-glycan structure	788:842	Mgat3 is a key glycosyltransferase in the synthesis of the bisecting (β1,4GlcNAc branching) N-glycan structure.					
29743543	7	51	theme	chains	1340:1345	arg1	formation					1318:1326	the branch formation	1307:1326	the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3	1307:1430	Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3.					
29743543	5	52	theme	miR-23a	909:915	arg1	target					899:904	a target	897:904	a target of miR-23a	897:915	Bioinformatics analysis indicated that Mgat3 may be a target of miR-23a, and this hypothesis was verified by dual-luciferase reporter gene assays.					
29743543	5	52	theme	miR-23a	909:915	arg1	Mgat3					884:888	Mgat3	884:888	Mgat3	884:888	Bioinformatics analysis indicated that Mgat3 may be a target of miR-23a, and this hypothesis was verified by dual-luciferase reporter gene assays.					
29743543	6	53	theme	ChIP	1178:1181	arg1	assays					1183:1188	ChIP assays	1178:1188	ChIP assays	1178:1188	Furthermore, we found that the transcription factor Runx2 can directly bind to the miR-23a gene promoter and promote its expression, as shown in dual-luciferase reporter gene assays and ChIP assays.					
29743543	2	54	theme	miR-23a	247:253	arg1	role					239:242	the role	235:242	the role of miR-23a in N-glycosylation	235:272	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	2	54	theme	miR-23a	247:253	arg1	metastasis					282:291	the metastasis	278:291	the metastasis of mouse hepatocellular carcinoma (HCC) cells	278:337	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	7	55	theme	glycosyltransferase	1406:1424	arg1	Mgat3					1426:1430	the glycosyltransferase Mgat3	1402:1430	the glycosyltransferase Mgat3	1402:1430	Collectively, these results indicate that miR-23a might increase the metastatic potential of mouse HCC by affecting the branch formation of N-glycan chains presented on the cell surface through the targeting of the glycosyltransferase Mgat3.					
29743543	3	56	theme	miR-23a	463:469	arg1	levels					482:487	higher miR-23a expression levels	456:487	higher miR-23a expression levels	456:487	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	3	57	theme	metastasis	520:529	arg1	potential					531:539	lymphatic metastasis potential	510:539	lymphatic metastasis potential	510:539	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	3	58	theme	mannoside	630:638	arg1	acetylglucosaminyltransferase					640:668	mannoside acetylglucosaminyltransferase 3	630:670	mannoside acetylglucosaminyltransferase 3 (Mgat3)	630:678	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	3	58	theme	mannoside	630:638	arg1	Mgat3					673:677	Mgat3	673:677	Mgat3	673:677	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	8	59	theme	abnormal	1498:1505	arg1	expression					1513:1522	abnormal miRNA expression	1498:1522	abnormal miRNA expression	1498:1522	These findings may provide insight into the relationship between abnormal miRNA expression and aberrant glycosylation during tumor lymphatic metastasis.					
29743543	2	60	theme	carcinoma	317:325	arg1	cells					333:337	mouse hepatocellular carcinoma (HCC) cells	296:337	mouse hepatocellular carcinoma (HCC) cells	296:337	In this study, the role of miR-23a in N-glycosylation and the metastasis of mouse hepatocellular carcinoma (HCC) cells was investigated.					
29743543	0	61	theme	mouse	77:81	arg1	cell					92:95	mouse hepatoma cell	77:95	mouse hepatoma cell	77:95	MiR-23a transcriptional activated by Runx2 increases metastatic potential of mouse hepatoma cell via directly targeting Mgat3.					
29743543	3	62	theme	acetylglucosaminyltransferase	640:668	arg1	expression					616:625	the expression	612:625	the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3)	612:678	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	3	63	theme	metastasis	583:592	arg1	potential					594:602	no lymphatic metastasis potential	570:602	no lymphatic metastasis potential	570:602	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	6	64	theme	gene	1162:1165	arg1	assays					1167:1172	dual-luciferase reporter gene assays	1137:1172	dual-luciferase reporter gene assays	1137:1172	Furthermore, we found that the transcription factor Runx2 can directly bind to the miR-23a gene promoter and promote its expression, as shown in dual-luciferase reporter gene assays and ChIP assays.					
29743543	8	65	theme	miRNA	1507:1511	arg1	expression					1513:1522	abnormal miRNA expression	1498:1522	abnormal miRNA expression	1498:1522	These findings may provide insight into the relationship between abnormal miRNA expression and aberrant glycosylation during tumor lymphatic metastasis.					
29743543	3	66	with	cells	558:562	arg1	potential					594:602	no lymphatic metastasis potential	570:602	no lymphatic metastasis potential	570:602	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
29743543	3	67	theme	expression	367:376	arg1	profiles					384:391	The miRNA expression array profiles	357:391	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses	357:445	The miRNA expression array profiles that were confirmed by qPCR and Western blot analyses revealed higher miR-23a expression levels in Hca-P cells (with lymphatic metastasis potential) than in Hepa1-6 cells (with no lymphatic metastasis potential), while the expression of mannoside acetylglucosaminyltransferase 3 (Mgat3) was negatively associated with metastasis potential.					
31328910	6	0	theme	nm	926:927	arg1	scaffolds					987:995	514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	917:995	514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	917:995	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	5	1	theme	mechanical	654:663	arg1	properties					665:674	The physicochemical and mechanical properties	630:674	The physicochemical and mechanical properties of samples	630:685	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	4	2	theme	simulated	602:610	arg1	fluid					617:621	simulated body fluid	602:621	simulated body fluid (SBF)	602:627	Apatite deposition was evaluated using simulated body fluid (SBF).					
31328910	4	2	theme	simulated	602:610	arg1	SBF					624:626	SBF	624:626	SBF	624:626	Apatite deposition was evaluated using simulated body fluid (SBF).					
31328910	6	3	theme	NF	901:902	arg1	diameters					904:912	NF diameters	901:912	NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	901:995	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	5	4	theme	physicochemical	634:648	arg1	properties					665:674	The physicochemical and mechanical properties	630:674	The physicochemical and mechanical properties of samples	630:685	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	2	5	theme	electrospun	317:327	arg1	NFs					341:343	NFs	341:343	NFs	341:343	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	2	5	theme	electrospun	317:327	arg1	nanofibers					329:338	electrospun nanofibers	317:338	electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing	317:462	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	8	6	theme	ions	1188:1191	arg1	consumption					1149:1159	the consumption	1145:1159	the consumption of calcium and phosphorous ions from SBF	1145:1200	This was confirmed by measuring the consumption of calcium and phosphorous ions from SBF.					
31328910	12	7	theme	contact	1662:1668	arg1	angle					1670:1674	contact angle	1662:1674	contact angle from 116.6 to 57.6°	1662:1694	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	1	8	theme	bone	201:204	arg1	matrix					220:225	bone extracellular matrix	201:225	bone extracellular matrix with osteogenic activity	201:250	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	7	9	from	Deposition	1012:1021	arg1	scaffolds					1045:1053	scaffolds	1045:1053	scaffolds	1045:1053	Deposition of apatite crystal on scaffolds started at week 2 followed by heavy deposition at week 8.					
31328910	9	10	theme	scaffolds	1224:1232	arg1	stability					1211:1219	Thermal stability	1203:1219	Thermal stability of scaffolds	1203:1232	Thermal stability of scaffolds was confirmed using DSC and TGA.					
31328910	1	11	theme	matrix	220:225	arg1	structure					172:180	structure	172:180	structure	172:180	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	1	11	theme	matrix	220:225	arg1	properties					187:196	properties	187:196	properties	187:196	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	1	11	theme	matrix	220:225	arg1	composition					159:169	composition	159:169	composition	159:169	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	15	12	from	solution	2030:2037	arg1	conclusion					1973:1982	conclusion	1973:1982	conclusion	1973:1982	In conclusion, the developed scaffolds could be a promising solution to stimulate bone regeneration.					
31328910	1	13	theme	biomaterials	124:135	arg1	range					115:119	a wide range	108:119	a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity	108:250	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	13	14	theme	scaffolds	1831:1839	arg1	cytocompatibility					1795:1811	the cytocompatibility	1791:1811	the cytocompatibility of the fabricated scaffolds	1791:1839	Cell viability study on mesenchymal stem cells proved the cytocompatibility of the fabricated scaffolds.					
31328910	7	15	theme	crystal	1034:1040	arg1	Deposition					1012:1021	Deposition	1012:1021	Deposition of apatite crystal on scaffolds	1012:1053	Deposition of apatite crystal on scaffolds started at week 2 followed by heavy deposition at week 8.					
31328910	13	16	theme	viability	1742:1750	arg1	study					1752:1756	Cell viability study	1737:1756	Cell viability study on mesenchymal stem cells	1737:1782	Cell viability study on mesenchymal stem cells proved the cytocompatibility of the fabricated scaffolds.					
31328910	10	17	from	layer	1288:1292	arg1	middle					1301:1306	the middle	1297:1306	the middle of the developed sandwich structure	1297:1342	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	5	18	dep	Fourier	704:710	arg1	transform					712:720	transform	712:720	transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine	712:835	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	5	19	theme	thermogravimetric	773:789	arg1	TGA					801:803	TGA	801:803	TGA	801:803	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	5	19	theme	thermogravimetric	773:789	arg1	analysis					791:798	thermogravimetric analysis	773:798	thermogravimetric analysis (TGA)	773:804	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	6	20	theme	scaffolds	987:995	arg1	diameters					904:912	NF diameters	901:912	NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	901:995	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	10	21	theme	±	1397:1397	arg1	MPa					1404:1406	12.224 ± 1.12 MPa	1390:1406	12.224 ± 1.12 MPa	1390:1406	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	5	22	theme	scanning	745:752	arg1	calorimetry					754:764	differential scanning calorimetry	732:764	differential scanning calorimetry (DSC)	732:770	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	5	22	theme	scanning	745:752	arg1	DSC					767:769	DSC	767:769	DSC	767:769	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	1	23	theme	bone	75:78	arg1	healing					80:86	Advanced bone healing	66:86	Advanced bone healing approaches	66:97	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	6	24	theme	sandwich-like	969:981	arg1	NFs					983:985	the sandwich-like NFs	965:985	the sandwich-like NFs	965:985	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	13	25	theme	stem	1773:1776	arg1	cells					1778:1782	mesenchymal stem cells	1761:1782	mesenchymal stem cells	1761:1782	Cell viability study on mesenchymal stem cells proved the cytocompatibility of the fabricated scaffolds.					
31328910	2	26	theme	oxide	404:408	arg1	composite					419:427	chitosan/polyethylene oxide (CS/PEO) composite	382:427	chitosan/polyethylene oxide (CS/PEO) composite	382:427	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	12	27	theme	scaffolds	1589:1597	arg1	layers					1575:1580	the outer NF layers	1562:1580	the outer NF layers of the scaffolds	1562:1597	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	2	28	theme	fracture	447:454	arg1	healing					456:462	bone fracture healing	442:462	bone fracture healing	442:462	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	10	29	theme	Young	1412:1416	arg1	modulus					1420:1426	Young's modulus	1412:1426	Young's modulus of 17.53 ±	1412:1437	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	1	30	dep	composition	159:169	arg1	the					155:157	the	155:157	the	155:157	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	0	31	theme	Sandwich-Like	0:12	arg1	Nanofibrous					14:24	Sandwich-Like Nanofibrous	0:24	Sandwich-Like Nanofibrous	0:24	Sandwich-Like Nanofibrous Scaffolds for Bone Tissue Regeneration.					
31328910	15	32	theme	promising	2020:2028	arg1	solution					2030:2037	a promising solution	2018:2037	a promising solution to stimulate bone regeneration	2018:2068	In conclusion, the developed scaffolds could be a promising solution to stimulate bone regeneration.					
31328910	15	32	theme	promising	2020:2028	arg1	scaffolds					1999:2007	the developed scaffolds	1985:2007	the developed scaffolds	1985:2007	In conclusion, the developed scaffolds could be a promising solution to stimulate bone regeneration.					
31328910	10	33	theme	NF	1285:1286	arg1	layer					1288:1292	the PCL NF layer	1277:1292	the PCL NF layer in the middle of the developed sandwich structure	1277:1342	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	5	34	theme	testing	821:827	arg1	machine					829:835	universal testing machine	811:835	universal testing machine	811:835	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	1	35	with	matrix	220:225	arg1	activity					243:250	osteogenic activity	232:250	osteogenic activity	232:250	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	6	36	theme	three-dimensional	868:884	arg1	structure					886:894	a porous three-dimensional structure	859:894	a porous three-dimensional structure	859:894	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	12	37	theme	NF	1572:1573	arg1	layers					1575:1580	the outer NF layers	1562:1580	the outer NF layers of the scaffolds	1562:1597	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	4	38	theme	Apatite	563:569	arg1	deposition					571:580	Apatite deposition	563:580	Apatite deposition	563:580	Apatite deposition was evaluated using simulated body fluid (SBF).					
31328910	0	39	theme	Bone	40:43	arg1	Regeneration					52:63	Bone Tissue Regeneration	40:63	Bone Tissue Regeneration	40:63	Sandwich-Like Nanofibrous Scaffolds for Bone Tissue Regeneration.					
31328910	14	40	theme	rabbit	1882:1887	arg1	model					1889:1893	in vivo mandibular bone defect rabbit model	1851:1893	in vivo mandibular bone defect rabbit model	1851:1893	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	12	41	theme	cell	1720:1723	arg1	attachment					1725:1734	cell attachment	1720:1734	cell attachment	1720:1734	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	12	42	from	reduction	1649:1657	arg1	57.6°					1690:1694	57.6°	1690:1694	57.6°	1690:1694	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	7	43	from	week	1105:1108	arg1	deposition					1091:1100	heavy deposition	1085:1100	heavy deposition at week 8	1085:1110	Deposition of apatite crystal on scaffolds started at week 2 followed by heavy deposition at week 8.					
31328910	12	44	attach	presence	1544:1551	arg2	CS					1556:1557	CS	1556:1557	CS	1556:1557	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	12	44	attach	presence	1544:1551	arg1	layers					1575:1580	the outer NF layers	1562:1580	the outer NF layers of the scaffolds	1562:1597	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	3	45	theme	fabricated	487:496	arg1	scaffolds					498:506	the fabricated scaffolds	483:506	the fabricated scaffolds	483:506	The morphology of the fabricated scaffolds was examined using scanning electron microscopy (SEM).					
31328910	14	46	theme	bone	1870:1873	arg1	model					1889:1893	in vivo mandibular bone defect rabbit model	1851:1893	in vivo mandibular bone defect rabbit model	1851:1893	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	14	47	theme	healthy	1941:1947	arg1	bone					1949:1952	a new healthy bone	1935:1952	a new healthy bone	1935:1952	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	15	48	theme	developed	1989:1997	arg1	solution					2030:2037	a promising solution	2018:2037	a promising solution to stimulate bone regeneration	2018:2068	In conclusion, the developed scaffolds could be a promising solution to stimulate bone regeneration.					
31328910	15	48	theme	developed	1989:1997	arg1	scaffolds					1999:2007	the developed scaffolds	1985:2007	the developed scaffolds	1985:2007	In conclusion, the developed scaffolds could be a promising solution to stimulate bone regeneration.					
31328910	4	49	theme	body	612:615	arg1	fluid					617:621	simulated body fluid	602:621	simulated body fluid (SBF)	602:627	Apatite deposition was evaluated using simulated body fluid (SBF).					
31328910	4	49	theme	body	612:615	arg1	SBF					624:626	SBF	624:626	SBF	624:626	Apatite deposition was evaluated using simulated body fluid (SBF).					
31328910	7	50	dep	started	1055:1061	arg1	followed					1073:1080	followed	1073:1080	followed by heavy deposition at week 8	1073:1110	Deposition of apatite crystal on scaffolds started at week 2 followed by heavy deposition at week 8.					
31328910	10	51	theme	sandwich	1325:1332	arg1	structure					1334:1342	the developed sandwich structure	1311:1342	the developed sandwich structure	1311:1342	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	10	52	theme	bear	1374:1377	arg1	load					1379:1382	bear load	1374:1382	bear load	1374:1382	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	10	53	with	scaffolds	1359:1367	arg1	load					1379:1382	bear load	1374:1382	bear load	1374:1382	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	14	54	used	used	1899:1902	arg2	model					1889:1893	in vivo mandibular bone defect rabbit model	1851:1893	in vivo mandibular bone defect rabbit model	1851:1893	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	2	55	theme	sandwich-like	290:302	arg1	structure					304:312	a sandwich-like structure	288:312	a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing	288:462	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	7	56	theme	heavy	1085:1089	arg1	deposition					1091:1100	heavy deposition	1085:1100	heavy deposition at week 8	1085:1110	Deposition of apatite crystal on scaffolds started at week 2 followed by heavy deposition at week 8.					
31328910	11	57	theme	porous	1464:1469	arg1	structure					1471:1479	The scaffolds' porous structure	1449:1479	The scaffolds' porous structure	1449:1479	The scaffolds' porous structure enhanced both cell propagation and proliferation.					
31328910	3	58	theme	electron	536:543	arg1	SEM					557:559	SEM	557:559	SEM	557:559	The morphology of the fabricated scaffolds was examined using scanning electron microscopy (SEM).					
31328910	3	58	theme	electron	536:543	arg1	microscopy					545:554	scanning electron microscopy	527:554	scanning electron microscopy (SEM)	527:560	The morphology of the fabricated scaffolds was examined using scanning electron microscopy (SEM).					
31328910	8	59	from	SBF	1198:1200	arg1	consumption					1149:1159	the consumption	1145:1159	the consumption of calcium and phosphorous ions from SBF	1145:1200	This was confirmed by measuring the consumption of calcium and phosphorous ions from SBF.					
31328910	2	60	theme	nanofibers	329:338	arg1	structure					304:312	a sandwich-like structure	288:312	a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing	288:462	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	15	61	theme	bone	2052:2055	arg1	regeneration					2057:2068	bone regeneration	2052:2068	bone regeneration	2052:2068	In conclusion, the developed scaffolds could be a promising solution to stimulate bone regeneration.					
31328910	8	62	theme	calcium	1164:1170	arg1	consumption					1149:1159	the consumption	1145:1159	the consumption of calcium and phosphorous ions from SBF	1145:1200	This was confirmed by measuring the consumption of calcium and phosphorous ions from SBF.					
31328910	12	63	theme	angle	1670:1674	arg1	reduction					1649:1657	the reduction	1645:1657	the reduction	1645:1657	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	12	63	theme	angle	1670:1674	arg1	essential					1706:1714	essential	1706:1714	essential	1706:1714	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	11	64	theme	cell	1495:1498	arg1	propagation					1500:1510	cell propagation	1495:1510	cell propagation	1495:1510	The scaffolds' porous structure enhanced both cell propagation and proliferation.					
31328910	8	65	theme	phosphorous	1176:1186	arg1	ions					1188:1191	phosphorous ions	1176:1191	phosphorous ions	1176:1191	This was confirmed by measuring the consumption of calcium and phosphorous ions from SBF.					
31328910	12	66	theme	CS	1556:1557	arg1	presence					1544:1551	the presence	1540:1551	the presence of CS in the outer NF layers of the scaffolds	1540:1597	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	14	67	dep	in	1851:1852	arg1	vivo					1854:1857	vivo	1854:1857	vivo	1854:1857	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	1	68	theme	extracellular	206:218	arg1	matrix					220:225	bone extracellular matrix	201:225	bone extracellular matrix with osteogenic activity	201:250	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	6	69	theme	PCL	947:949	arg1	NFs					951:953	PCL NFs	947:953	PCL NFs	947:953	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	5	70	theme	samples	679:685	arg1	properties					665:674	The physicochemical and mechanical properties	630:674	The physicochemical and mechanical properties of samples	630:685	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	6	71	theme	nm	940:941	arg1	scaffolds					987:995	514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	917:995	514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	917:995	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	13	72	theme	fabricated	1820:1829	arg1	scaffolds					1831:1839	the fabricated scaffolds	1816:1839	the fabricated scaffolds	1816:1839	Cell viability study on mesenchymal stem cells proved the cytocompatibility of the fabricated scaffolds.					
31328910	9	73	theme	Thermal	1203:1209	arg1	stability					1211:1219	Thermal stability	1203:1219	Thermal stability of scaffolds	1203:1232	Thermal stability of scaffolds was confirmed using DSC and TGA.					
31328910	7	74	theme	apatite	1026:1032	arg1	crystal					1034:1040	apatite crystal	1026:1040	apatite crystal	1026:1040	Deposition of apatite crystal on scaffolds started at week 2 followed by heavy deposition at week 8.					
31328910	2	75	theme	present	257:263	arg1	study					265:269	The present study	253:269	The present study	253:269	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	12	76	dep	57.6°	1690:1694	arg1	to					1687:1688	to	1687:1688	to	1687:1688	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	6	77	theme	layer	955:959	arg1	scaffolds					987:995	514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	917:995	514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	917:995	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	5	78	dep	transform	712:720	arg1	infrared					722:729	infrared	722:729	infrared	722:729	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	5	79	theme	differential	732:743	arg1	calorimetry					754:764	differential scanning calorimetry	732:764	differential scanning calorimetry (DSC)	732:770	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	5	79	theme	differential	732:743	arg1	DSC					767:769	DSC	767:769	DSC	767:769	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	1	80	theme	Advanced	66:73	arg1	healing					80:86	Advanced bone healing	66:86	Advanced bone healing approaches	66:97	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	6	81	theme	NFs	983:985	arg1	scaffolds					987:995	514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	917:995	514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds	917:995	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	10	82	theme	12.224	1390:1395	arg1	MPa					1404:1406	12.224 ± 1.12 MPa	1390:1406	12.224 ± 1.12 MPa	1390:1406	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	13	83	theme	mesenchymal	1761:1771	arg1	cells					1778:1782	mesenchymal stem cells	1761:1782	mesenchymal stem cells	1761:1782	Cell viability study on mesenchymal stem cells proved the cytocompatibility of the fabricated scaffolds.					
31328910	1	84	theme	healing	80:86	arg1	approaches					88:97	Advanced bone healing approaches	66:97	Advanced bone healing approaches	66:97	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	12	85	from	57.6°	1690:1694	arg1	reduction					1649:1657	the reduction	1645:1657	the reduction	1645:1657	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	12	85	from	57.6°	1690:1694	arg1	angle					1670:1674	contact angle	1662:1674	contact angle from 116.6 to 57.6°	1662:1694	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	12	85	from	57.6°	1690:1694	arg1	essential					1706:1714	essential	1706:1714	essential	1706:1714	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	2	86	theme	chitosan/polyethylene	382:402	arg1	CS/PEO					411:416	CS/PEO	411:416	CS/PEO	411:416	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	2	86	theme	chitosan/polyethylene	382:402	arg1	oxide					404:408	chitosan/polyethylene oxide	382:408	chitosan/polyethylene oxide (CS/PEO) composite	382:427	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	2	87	theme	bone	442:445	arg1	healing					456:462	bone fracture healing	442:462	bone fracture healing	442:462	The present study aimed to develop a sandwich-like structure of electrospun nanofibers (NFs) based on polycaprolactone (PCL) and chitosan/polyethylene oxide (CS/PEO) composite to stimulate bone fracture healing.					
31328910	13	88	theme	Cell	1737:1740	arg1	study					1752:1756	Cell viability study	1737:1756	Cell viability study on mesenchymal stem cells	1737:1782	Cell viability study on mesenchymal stem cells proved the cytocompatibility of the fabricated scaffolds.					
31328910	5	89	theme	universal	811:819	arg1	machine					829:835	universal testing machine	811:835	universal testing machine	811:835	The physicochemical and mechanical properties of samples were analyzed by Fourier transform infrared, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and universal testing machine.					
31328910	1	90	theme	wide	110:113	arg1	range					115:119	a wide range	108:119	a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity	108:250	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	10	91	theme	±	1437:1437	arg1	modulus					1420:1426	Young's modulus	1412:1426	Young's modulus of 17.53 ±	1412:1437	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	10	92	theme	PCL	1281:1283	arg1	layer					1288:1292	the PCL NF layer	1277:1292	the PCL NF layer in the middle of the developed sandwich structure	1277:1342	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	0	93	theme	Tissue	45:50	arg1	Regeneration					52:63	Bone Tissue Regeneration	40:63	Bone Tissue Regeneration	40:63	Sandwich-Like Nanofibrous Scaffolds for Bone Tissue Regeneration.					
31328910	12	94	from	presence	1544:1551	arg1	layers					1575:1580	the outer NF layers	1562:1580	the outer NF layers of the scaffolds	1562:1597	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	6	95	theme	porous	861:866	arg1	structure					886:894	a porous three-dimensional structure	859:894	a porous three-dimensional structure	859:894	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	13	96	from	study	1752:1756	arg1	cells					1778:1782	mesenchymal stem cells	1761:1782	mesenchymal stem cells	1761:1782	Cell viability study on mesenchymal stem cells proved the cytocompatibility of the fabricated scaffolds.					
31328910	12	97	theme	outer	1566:1570	arg1	layers					1575:1580	the outer NF layers	1562:1580	the outer NF layers of the scaffolds	1562:1597	Besides, the presence of CS in the outer NF layers of the scaffolds increased the hydrophilicity, as evidenced by the reduction of contact angle from 116.6 to 57.6°, which is essential for cell attachment.					
31328910	10	98	dep	MPa	1404:1406	arg1	up					1384:1385	up	1384:1385	up	1384:1385	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	10	98	dep	MPa	1404:1406	arg1	MPa					1444:1446	3.24 MPa	1439:1446	3.24 MPa	1439:1446	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	14	99	theme	defect	1875:1880	arg1	model					1889:1893	in vivo mandibular bone defect rabbit model	1851:1893	in vivo mandibular bone defect rabbit model	1851:1893	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	14	100	theme	bone	1949:1952	arg1	regeneration					1919:1930	the regeneration	1915:1930	the regeneration of a new healthy bone within 28 days	1915:1967	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	3	101	theme	scaffolds	498:506	arg1	morphology					469:478	The morphology	465:478	The morphology of the fabricated scaffolds	465:506	The morphology of the fabricated scaffolds was examined using scanning electron microscopy (SEM).					
31328910	6	102	theme	SEM	838:840	arg1	images					842:847	SEM images	838:847	SEM images	838:847	SEM images exhibited a porous three-dimensional structure with NF diameters of 514-4745 nm and 68-786 nm for PCL NFs layer and the sandwich-like NFs scaffolds, respectively.					
31328910	14	103	theme	mandibular	1859:1868	arg1	model					1889:1893	in vivo mandibular bone defect rabbit model	1851:1893	in vivo mandibular bone defect rabbit model	1851:1893	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	14	104	theme	new	1937:1939	arg1	bone					1949:1952	a new healthy bone	1935:1952	a new healthy bone	1935:1952	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	10	105	theme	structure	1334:1342	arg1	middle					1301:1306	the middle	1297:1306	the middle of the developed sandwich structure	1297:1342	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	1	106	theme	osteogenic	232:241	arg1	activity					243:250	osteogenic activity	232:250	osteogenic activity	232:250	Advanced bone healing approaches included a wide range of biomaterials that mainly mimic the composition, structure, and properties of bone extracellular matrix with osteogenic activity.					
31328910	14	107	theme	in	1851:1852	arg1	model					1889:1893	in vivo mandibular bone defect rabbit model	1851:1893	in vivo mandibular bone defect rabbit model	1851:1893	Finally, in vivo mandibular bone defect rabbit model was used to confirm the regeneration of a new healthy bone within 28 days.					
31328910	10	108	theme	developed	1315:1323	arg1	structure					1334:1342	the developed sandwich structure	1311:1342	the developed sandwich structure	1311:1342	Moreover, the PCL NF layer in the middle of the developed sandwich structure reinforced the scaffolds with bear load up to 12.224 ± 1.12 MPa and Young's modulus of 17.53 ± 3.24 MPa.					
31328910	3	109	theme	scanning	527:534	arg1	SEM					557:559	SEM	557:559	SEM	557:559	The morphology of the fabricated scaffolds was examined using scanning electron microscopy (SEM).					
31328910	3	109	theme	scanning	527:534	arg1	microscopy					545:554	scanning electron microscopy	527:554	scanning electron microscopy (SEM)	527:560	The morphology of the fabricated scaffolds was examined using scanning electron microscopy (SEM).					
30601102	3	0	theme	ecoimmunological	325:340	arg1	unclear					407:413	unclear	407:413	unclear	407:413	However, the ecoimmunological impacts of this supplementation on free-living birds are largely unclear.					
30601102	3	0	theme	ecoimmunological	325:340	arg1	impacts					342:348	the ecoimmunological impacts	321:348	the ecoimmunological impacts of this supplementation on free-living birds	321:393	However, the ecoimmunological impacts of this supplementation on free-living birds are largely unclear.					
30601102	5	1	contain	had	754:756	arg1	groups					747:752	all three groups	737:752	all three groups	737:752	At capture, all three groups had similar body mass and fat scores.					
30601102	5	1	contain	had	754:756	arg2	scores					784:789	fat scores	780:789	fat scores	780:789	At capture, all three groups had similar body mass and fat scores.					
30601102	5	1	contain	had	754:756	arg2	mass					771:774	similar body mass	758:774	similar body mass	758:774	At capture, all three groups had similar body mass and fat scores.					
30601102	6	2	theme	Y	863:863	arg1	antibodies					865:874	circulating immunoglobulin Y antibodies	836:874	circulating immunoglobulin Y antibodies	836:874	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	1	3	theme	wild	166:169	arg1	birds					171:175	wild birds	166:175	wild birds	166:175	Food availability might sometimes be unpredictable for wild birds.					
30601102	6	4	theme	complement	880:889	arg1	ability					897:903	complement lysis ability	880:903	complement lysis ability	880:903	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	6	4	theme	complement	880:889	arg1	measures					910:917	two measures	906:917	two measures of constitutive immune function	906:949	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	4	5	theme	supplemented	612:623	arg1	"					624:624	supplemented"	612:624	supplemented"	612:624	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	5	theme	supplemented	612:623	arg1	supplementation					594:608	constant food supplementation	580:608	constant food supplementation ("supplemented")	580:625	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	5	6	theme	similar	758:764	arg1	mass					771:774	similar body mass	758:774	similar body mass	758:774	At capture, all three groups had similar body mass and fat scores.					
30601102	2	7	theme	food	205:208	arg1	limitation					210:219	this possible food limitation	191:219	this possible food limitation	191:219	To alleviate this possible food limitation, millions of households in North America provide food supplementation to bird populations.					
30601102	8	8	theme	supplementation	1339:1353	arg1	level					1325:1329	their level	1319:1329	their level of food supplementation	1319:1353	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	0	9	theme	Supplementation	94:108	arg1	Gradient					77:84	a Gradient	75:84	a Gradient of Food Supplementation	75:108	Physiological and Immune Responses of Free-Living Temperate Birds Provided a Gradient of Food Supplementation.					
30601102	6	10	theme	lysis	891:895	arg1	ability					897:903	complement lysis ability	880:903	complement lysis ability	880:903	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	6	10	theme	lysis	891:895	arg1	measures					910:917	two measures	906:917	two measures of constitutive immune function	906:949	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	7	11	theme	interrupted	1224:1234	arg1	group					1236:1240	the interrupted group	1220:1240	the interrupted group	1220:1240	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	8	12	theme	food	1396:1399	arg1	removal					1372:1378	sudden removal	1365:1378	sudden removal of supplemental food	1365:1399	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	0	13	theme	Food	89:92	arg1	Supplementation					94:108	Food Supplementation	89:108	Food Supplementation	89:108	Physiological and Immune Responses of Free-Living Temperate Birds Provided a Gradient of Food Supplementation.					
30601102	4	14	theme	free-living	495:505	arg1	Poecile					532:538	Poecile	532:538	Poecile	532:538	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	14	theme	free-living	495:505	arg1	chickadees					520:529	free-living black-capped chickadees	495:529	free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented")	495:722	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	8	15	theme	sudden	1365:1370	arg1	removal					1372:1378	sudden removal	1365:1378	sudden removal of supplemental food	1365:1399	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	7	16	theme	acute-phase	1146:1156	arg1	response					1167:1174	a higher haptoglobin (acute-phase protein) response	1124:1174	a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group	1124:1240	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	7	16	theme	acute-phase	1146:1156	arg1	protein					1158:1164	acute-phase protein	1146:1164	acute-phase protein	1146:1164	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	2	17	theme	bird	294:297	arg1	populations					299:309	bird populations	294:309	bird populations	294:309	To alleviate this possible food limitation, millions of households in North America provide food supplementation to bird populations.					
30601102	8	18	from	decline	1427:1433	arg1	aspects					1438:1444	aspects	1438:1444	aspects of immunity	1438:1456	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	8	19	theme	similar	1289:1295	arg1	levels					1297:1302	similar levels	1289:1302	similar levels of fat	1289:1309	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	3	20	theme	supplementation	358:372	arg1	unclear					407:413	unclear	407:413	unclear	407:413	However, the ecoimmunological impacts of this supplementation on free-living birds are largely unclear.					
30601102	3	20	theme	supplementation	358:372	arg1	impacts					342:348	the ecoimmunological impacts	321:348	the ecoimmunological impacts of this supplementation on free-living birds	321:393	However, the ecoimmunological impacts of this supplementation on free-living birds are largely unclear.					
30601102	8	21	theme	fat	1307:1309	arg1	levels					1297:1302	similar levels	1289:1302	similar levels of fat	1289:1309	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	2	22	from	millions	222:229	arg1	America					254:260	America	254:260	America	254:260	To alleviate this possible food limitation, millions of households in North America provide food supplementation to bird populations.					
30601102	6	23	contain	had	814:816	arg2	measures					910:917	two measures	906:917	two measures of constitutive immune function	906:949	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	6	23	contain	had	814:816	arg1	groups					802:807	All three groups	792:807	All three groups	792:807	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	6	23	contain	had	814:816	arg2	ability					897:903	complement lysis ability	880:903	complement lysis ability	880:903	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	6	23	contain	had	814:816	arg2	levels					826:831	similar levels	818:831	similar levels of circulating immunoglobulin Y antibodies	818:874	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	5	24	theme	fat	780:782	arg1	scores					784:789	fat scores	780:789	fat scores	780:789	At capture, all three groups had similar body mass and fat scores.					
30601102	4	25	theme	chickadees	520:529	arg1	Poecile					532:538	Poecile	532:538	Poecile	532:538	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	25	theme	chickadees	520:529	arg1	chickadees					520:529	free-living black-capped chickadees	495:529	free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented")	495:722	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	25	theme	chickadees	520:529	arg1	groups					485:490	three groups	479:490	three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented")	479:722	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	5	26	theme	body	766:769	arg1	mass					771:774	similar body mass	758:774	similar body mass	758:774	At capture, all three groups had similar body mass and fat scores.					
30601102	7	27	theme	lipopolysaccharide	1179:1196	arg1	injection					1198:1206	lipopolysaccharide injection	1179:1206	lipopolysaccharide injection	1179:1206	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	9	28	theme	Future	1459:1464	arg1	studies					1466:1472	Future studies	1459:1472	Future studies	1459:1472	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	4	29	theme	black-capped	507:518	arg1	Poecile					532:538	Poecile	532:538	Poecile	532:538	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	29	theme	black-capped	507:518	arg1	chickadees					520:529	free-living black-capped chickadees	495:529	free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented")	495:722	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	8	30	theme	supplemental	1383:1394	arg1	food					1396:1399	supplemental food	1383:1399	supplemental food	1383:1399	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	2	31	from	households	234:243	arg1	America					254:260	America	254:260	America	254:260	To alleviate this possible food limitation, millions of households in North America provide food supplementation to bird populations.					
30601102	0	32	theme	Physiological	0:12	arg1	Responses					25:33	Physiological and Immune Responses	0:33	Physiological and Immune Responses of Free-Living Temperate Birds	0:64	Physiological and Immune Responses of Free-Living Temperate Birds Provided a Gradient of Food Supplementation.					
30601102	4	33	theme	food	589:592	arg1	"					624:624	supplemented"	612:624	supplemented"	612:624	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	33	theme	food	589:592	arg1	supplementation					594:608	constant food supplementation	580:608	constant food supplementation ("supplemented")	580:625	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	2	34	from	America	254:260	arg1	millions					222:229	millions	222:229	millions of households in North America	222:260	To alleviate this possible food limitation, millions of households in North America provide food supplementation to bird populations.					
30601102	7	35	theme	higher	1126:1131	arg1	response					1167:1174	a higher haptoglobin (acute-phase protein) response	1124:1174	a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group	1124:1240	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	7	35	theme	higher	1126:1131	arg1	protein					1158:1164	acute-phase protein	1146:1164	acute-phase protein	1146:1164	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	4	36	theme	constant	580:587	arg1	"					624:624	supplemented"	612:624	supplemented"	612:624	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	36	theme	constant	580:587	arg1	supplementation					594:608	constant food supplementation	580:608	constant food supplementation ("supplemented")	580:625	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	8	37	theme	short-term	1416:1425	arg1	decline					1427:1433	a short-term decline	1414:1433	a short-term decline in aspects of immunity	1414:1456	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	7	38	theme	supplemented	1101:1112	arg1	group					1114:1118	the supplemented group	1097:1118	the supplemented group	1097:1118	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	7	39	with	injection	1053:1061	arg1	lipopolysaccharide					1068:1085	lipopolysaccharide	1068:1085	lipopolysaccharide	1068:1085	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	0	40	theme	Immune	18:23	arg1	Responses					25:33	Physiological and Immune Responses	0:33	Physiological and Immune Responses of Free-Living Temperate Birds	0:64	Physiological and Immune Responses of Free-Living Temperate Birds Provided a Gradient of Food Supplementation.					
30601102	6	41	theme	immunoglobulin	848:861	arg1	antibodies					865:874	circulating immunoglobulin Y antibodies	836:874	circulating immunoglobulin Y antibodies	836:874	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	1	42	theme	Food	111:114	arg1	availability					116:127	Food availability	111:127	Food availability	111:127	Food availability might sometimes be unpredictable for wild birds.					
30601102	9	43	theme	immune	1562:1567	arg1	function					1569:1576	immune function	1562:1576	immune function	1562:1576	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	9	44	theme	adaptive	1542:1549	arg1	aspects					1551:1557	induced or adaptive aspects	1531:1557	induced or adaptive aspects of immune function	1531:1576	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	0	45	theme	Free-Living	38:48	arg1	Birds					60:64	Free-Living Temperate Birds	38:64	Free-Living Temperate Birds	38:64	Physiological and Immune Responses of Free-Living Temperate Birds Provided a Gradient of Food Supplementation.					
30601102	6	46	theme	function	942:949	arg1	ability					897:903	complement lysis ability	880:903	complement lysis ability	880:903	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	6	46	theme	function	942:949	arg1	measures					910:917	two measures	906:917	two measures of constitutive immune function	906:949	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	6	47	theme	circulating	836:846	arg1	antibodies					865:874	circulating immunoglobulin Y antibodies	836:874	circulating immunoglobulin Y antibodies	836:874	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	9	48	theme	free-living	1623:1633	arg1	animals					1635:1641	free-living animals	1623:1641	free-living animals	1623:1641	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	9	49	theme	food	1497:1500	arg1	supplementation					1502:1516	food supplementation	1497:1516	food supplementation	1497:1516	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	6	50	theme	immune	935:940	arg1	function					942:949	constitutive immune function	922:949	constitutive immune function	922:949	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	4	51	theme	groups	485:490	arg1	function					446:453	immune function	439:453	immune function	439:453	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	51	theme	groups	485:490	arg1	composition					464:474	body composition	459:474	body composition	459:474	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	2	52	theme	households	234:243	arg1	millions					222:229	millions	222:229	millions of households in North America	222:260	To alleviate this possible food limitation, millions of households in North America provide food supplementation to bird populations.					
30601102	7	53	theme	body	1015:1018	arg1	mass					1020:1023	a somewhat similar body mass	996:1023	a somewhat similar body mass	996:1023	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	4	54	theme	interrupted	663:673	arg1	"					674:674	"interrupted"	662:674	"interrupted"	662:674	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	54	theme	interrupted	663:673	arg1	supplementation					645:659	interrupted food supplementation	628:659	interrupted food supplementation ("interrupted")	628:675	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	0	55	theme	Birds	60:64	arg1	Responses					25:33	Physiological and Immune Responses	0:33	Physiological and Immune Responses of Free-Living Temperate Birds	0:64	Physiological and Immune Responses of Free-Living Temperate Birds Provided a Gradient of Food Supplementation.					
30601102	3	56	theme	free-living	377:387	arg1	birds					389:393	free-living birds	377:393	free-living birds	377:393	However, the ecoimmunological impacts of this supplementation on free-living birds are largely unclear.					
30601102	9	57	from	animals	1635:1641	arg1	understanding					1592:1604	our understanding	1588:1604	our understanding of immunology in free-living animals	1588:1641	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	6	58	theme	antibodies	865:874	arg1	ability					897:903	complement lysis ability	880:903	complement lysis ability	880:903	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	6	58	theme	antibodies	865:874	arg1	measures					910:917	two measures	906:917	two measures of constitutive immune function	906:949	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	6	58	theme	antibodies	865:874	arg1	levels					826:831	similar levels	818:831	similar levels of circulating immunoglobulin Y antibodies	818:874	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	0	59	theme	Temperate	50:58	arg1	Birds					60:64	Free-Living Temperate Birds	38:64	Free-Living Temperate Birds	38:64	Physiological and Immune Responses of Free-Living Temperate Birds Provided a Gradient of Food Supplementation.					
30601102	4	60	dep	Poecile	532:538	arg1	atricapillus					540:551	Poecile atricapillus	532:551	Poecile atricapillus	532:551	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	6	61	theme	similar	818:824	arg1	levels					826:831	similar levels	818:831	similar levels of circulating immunoglobulin Y antibodies	818:874	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	4	62	theme	immune	439:444	arg1	function					446:453	immune function	439:453	immune function	439:453	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	63	theme	unsupplemented	707:720	arg1	"					721:721	"unsupplemented"	706:721	"unsupplemented"	706:721	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	63	theme	unsupplemented	707:720	arg1	supplementation					689:703	no food supplementation	681:703	no food supplementation ("unsupplemented")	681:722	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	3	64	from	impacts	342:348	arg1	birds					389:393	free-living birds	377:393	free-living birds	377:393	However, the ecoimmunological impacts of this supplementation on free-living birds are largely unclear.					
30601102	2	65	theme	food	270:273	arg1	supplementation					275:289	food supplementation	270:289	food supplementation to bird populations	270:309	To alleviate this possible food limitation, millions of households in North America provide food supplementation to bird populations.					
30601102	7	66	theme	similar	1007:1013	arg1	mass					1020:1023	a somewhat similar body mass	996:1023	a somewhat similar body mass	996:1023	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	9	67	theme	immunology	1609:1618	arg1	understanding					1592:1604	our understanding	1588:1604	our understanding of immunology in free-living animals	1588:1641	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	9	68	from	immunology	1609:1618	arg1	animals					1635:1641	free-living animals	1623:1641	free-living animals	1623:1641	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	4	69	theme	interrupted	628:638	arg1	"					674:674	"interrupted"	662:674	"interrupted"	662:674	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	69	theme	interrupted	628:638	arg1	supplementation					645:659	interrupted food supplementation	628:659	interrupted food supplementation ("interrupted")	628:675	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	7	70	theme	temperature	1029:1039	arg1	response					1041:1048	temperature response	1029:1048	temperature response to injection with lipopolysaccharide	1029:1085	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	4	71	theme	body	459:462	arg1	composition					464:474	body composition	459:474	body composition	459:474	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	9	72	theme	function	1569:1576	arg1	aspects					1551:1557	induced or adaptive aspects	1531:1557	induced or adaptive aspects of immune function	1531:1576	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	9	73	theme	induced	1531:1537	arg1	aspects					1551:1557	induced or adaptive aspects	1531:1557	induced or adaptive aspects of immune function	1531:1576	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	2	74	theme	possible	196:203	arg1	limitation					210:219	this possible food limitation	191:219	this possible food limitation	191:219	To alleviate this possible food limitation, millions of households in North America provide food supplementation to bird populations.					
30601102	8	75	theme	immunity	1449:1456	arg1	aspects					1438:1444	aspects	1438:1444	aspects of immunity	1438:1456	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	7	76	contain	had	1120:1122	arg2	response					1167:1174	a higher haptoglobin (acute-phase protein) response	1124:1174	a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group	1124:1240	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	7	76	contain	had	1120:1122	arg2	protein					1158:1164	acute-phase protein	1146:1164	acute-phase protein	1146:1164	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	7	76	contain	had	1120:1122	arg1	group					1114:1118	the supplemented group	1097:1118	the supplemented group	1097:1118	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	8	77	theme	food	1334:1337	arg1	supplementation					1339:1353	food supplementation	1334:1353	food supplementation	1334:1353	This study demonstrates that birds maintained similar levels of fat despite their level of food supplementation; however, sudden removal of supplemental food might elicit a short-term decline in aspects of immunity.					
30601102	9	78	from	understanding	1592:1604	arg1	animals					1635:1641	free-living animals	1623:1641	free-living animals	1623:1641	Future studies should investigate how food supplementation might impact induced or adaptive aspects of immune function to add to our understanding of immunology in free-living animals.					
30601102	7	79	theme	haptoglobin	1133:1143	arg1	response					1167:1174	a higher haptoglobin (acute-phase protein) response	1124:1174	a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group	1124:1240	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	7	79	theme	haptoglobin	1133:1143	arg1	protein					1158:1164	acute-phase protein	1146:1164	acute-phase protein	1146:1164	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	7	80	theme	interrupted	969:979	arg1	groups					981:986	interrupted groups	969:986	interrupted groups	969:986	Supplemented and interrupted groups mounted a somewhat similar body mass and temperature response to injection with lipopolysaccharide; however, the supplemented group had a higher haptoglobin (acute-phase protein) response to lipopolysaccharide injection compared to the interrupted group.					
30601102	4	81	theme	food	684:687	arg1	"					721:721	"unsupplemented"	706:721	"unsupplemented"	706:721	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	81	theme	food	684:687	arg1	supplementation					689:703	no food supplementation	681:703	no food supplementation ("unsupplemented")	681:722	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	6	82	theme	constitutive	922:933	arg1	function					942:949	constitutive immune function	922:949	constitutive immune function	922:949	All three groups also had similar levels of circulating immunoglobulin Y antibodies and complement lysis ability, two measures of constitutive immune function.					
30601102	4	83	theme	food	640:643	arg1	"					674:674	"interrupted"	662:674	"interrupted"	662:674	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30601102	4	83	theme	food	640:643	arg1	supplementation					645:659	interrupted food supplementation	628:659	interrupted food supplementation ("interrupted")	628:675	Therefore, we compared immune function and body composition of three groups of free-living black-capped chickadees (Poecile atricapillus) that were provided either constant food supplementation ("supplemented"), interrupted food supplementation ("interrupted"), or no food supplementation ("unsupplemented").					
30447366	7	0	theme	wound	1492:1496	arg1	healing					1498:1504	accelerated wound healing	1480:1504	accelerated wound healing	1480:1504	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	5	1	dep	decreased	1030:1038	arg1	compared					1040:1047	compared	1040:1047	decreased compared to Alg hydrogel	1030:1063	The antibacterial property of the composite hydrogel against E. coli and S. aureus was confirmed through colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel.					
30447366	4	2	theme	CaF2	716:719	arg1	content					721:727	CaF2 content	716:727	CaF2 content inside the composite hydrogel	716:757	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	4	3	from	amount	621:626	arg1	hydrogel					681:688	the nanocomposite hydrogel	663:688	the nanocomposite hydrogel	663:688	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	7	4	theme	accelerated	1480:1490	arg1	healing					1498:1504	accelerated wound healing	1480:1504	accelerated wound healing	1480:1504	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	6	5	theme	wound	1112:1116	arg1	model					1118:1122	a full-thickness wound model	1095:1122	a full-thickness wound model	1095:1122	The in vivo results based on a full-thickness wound model showed that the nanocomposite hydrogel effectively enhanced the deposition of the extracellular matrix compared to that of the Alg hydrogel.					
30447366	6	6	dep	in	1070:1071	arg1	vivo					1073:1076	vivo	1073:1076	vivo	1073:1076	The in vivo results based on a full-thickness wound model showed that the nanocomposite hydrogel effectively enhanced the deposition of the extracellular matrix compared to that of the Alg hydrogel.					
30447366	6	7	theme	in	1070:1071	arg1	results					1078:1084	The in vivo results	1066:1084	The in vivo results based on a full-thickness wound model	1066:1122	The in vivo results based on a full-thickness wound model showed that the nanocomposite hydrogel effectively enhanced the deposition of the extracellular matrix compared to that of the Alg hydrogel.					
30447366	5	8	theme	colony	955:960	arg1	formation					962:970	colony formation	955:970	colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel	955:1063	The antibacterial property of the composite hydrogel against E. coli and S. aureus was confirmed through colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel.					
30447366	3	9	dep	nano-sized	507:516	arg1	90 nm					525:529	90 nm	525:529	90 nm	525:529	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	2	10	theme	excellent	306:314	arg1	candidate					316:324	an excellent candidate	303:324	an excellent candidate for injectable wound dressing materials	303:364	Alginate (Alg) is an excellent candidate for injectable wound dressing materials because it can form a gel in a mild environment.					
30447366	2	10	theme	excellent	306:314	arg1	Alginate					285:292	Alginate	285:292	Alginate (Alg)	285:298	Alginate (Alg) is an excellent candidate for injectable wound dressing materials because it can form a gel in a mild environment.					
30447366	4	11	from	hydrogel	681:688	arg1	ion					654:656	released fluorine (F-) ion	631:656	released fluorine (F-) ion from the nanocomposite hydrogel	631:688	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	4	11	from	hydrogel	681:688	arg1	amount					621:626	The amount	617:626	The amount of released fluorine (F-) ion from the nanocomposite hydrogel	617:688	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	3	12	theme	nano-sized	507:516	arg1	particles					556:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	3	13	contain	containing	496:505	arg2	particles					556:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	3	13	contain	containing	496:505	arg1	hydrogel					487:494	an injectable nano composite hydrogel	458:494	an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles	458:564	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	7	14	theme	cell	1443:1446	arg1	proliferation					1448:1460	cell proliferation	1443:1460	cell proliferation	1443:1460	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	5	15	theme	antibacterial	854:866	arg1	property					868:875	The antibacterial property	850:875	The antibacterial property of the composite hydrogel against E. coli and S. aureus	850:931	The antibacterial property of the composite hydrogel against E. coli and S. aureus was confirmed through colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel.					
30447366	6	16	theme	extracellular	1206:1218	arg1	matrix					1220:1225	the extracellular matrix	1202:1225	the extracellular matrix	1202:1225	The in vivo results based on a full-thickness wound model showed that the nanocomposite hydrogel effectively enhanced the deposition of the extracellular matrix compared to that of the Alg hydrogel.					
30447366	3	17	theme	gelation	439:446	arg1	property					448:455	its gelation property	435:455	its gelation property	435:455	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	6	18	theme	full-thickness	1097:1110	arg1	model					1118:1122	a full-thickness wound model	1095:1122	a full-thickness wound model	1095:1122	The in vivo results based on a full-thickness wound model showed that the nanocomposite hydrogel effectively enhanced the deposition of the extracellular matrix compared to that of the Alg hydrogel.					
30447366	6	19	theme	nanocomposite	1140:1152	arg1	hydrogel					1154:1161	the nanocomposite hydrogel	1136:1161	the nanocomposite hydrogel	1136:1161	The in vivo results based on a full-thickness wound model showed that the nanocomposite hydrogel effectively enhanced the deposition of the extracellular matrix compared to that of the Alg hydrogel.					
30447366	7	20	theme	injectable	1349:1358	arg1	material					1375:1382	a bioactive injectable wound-dressing material	1337:1382	a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing	1337:1504	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	7	20	theme	injectable	1349:1358	arg1	potential					1293:1301	the potential	1289:1301	the potential of this nanocomposite hydrogel	1289:1332	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	3	21	theme	CaF2	550:553	arg1	particles					556:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	5	22	theme	composite	884:892	arg1	hydrogel					894:901	the composite hydrogel	880:901	the composite hydrogel against E. coli and S. aureus	880:931	The antibacterial property of the composite hydrogel against E. coli and S. aureus was confirmed through colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel.					
30447366	0	23	theme	injectable	23:32	arg1	hydrogel					57:64	injectable alginate nanocomposite hydrogel	23:64	injectable alginate nanocomposite hydrogel	23:64	Fluorine-ion-releasing injectable alginate nanocomposite hydrogel for enhanced bioactivity and antibacterial property.					
30447366	2	24	theme	dressing	347:354	arg1	materials					356:364	injectable wound dressing materials	330:364	injectable wound dressing materials	330:364	Alginate (Alg) is an excellent candidate for injectable wound dressing materials because it can form a gel in a mild environment.					
30447366	2	25	theme	mild	397:400	arg1	environment					402:412	a mild environment	395:412	a mild environment	395:412	Alginate (Alg) is an excellent candidate for injectable wound dressing materials because it can form a gel in a mild environment.					
30447366	2	26	theme	wound	341:345	arg1	materials					356:364	injectable wound dressing materials	330:364	injectable wound dressing materials	330:364	Alginate (Alg) is an excellent candidate for injectable wound dressing materials because it can form a gel in a mild environment.					
30447366	1	27	theme	antibacterial	219:231	arg1	property					233:240	antibacterial property	219:240	antibacterial property	219:240	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	0	28	theme	nanocomposite	43:55	arg1	hydrogel					57:64	injectable alginate nanocomposite hydrogel	23:64	injectable alginate nanocomposite hydrogel	23:64	Fluorine-ion-releasing injectable alginate nanocomposite hydrogel for enhanced bioactivity and antibacterial property.					
30447366	6	29	theme	Alg	1251:1253	arg1	hydrogel					1255:1262	the Alg hydrogel	1247:1262	the Alg hydrogel	1247:1262	The in vivo results based on a full-thickness wound model showed that the nanocomposite hydrogel effectively enhanced the deposition of the extracellular matrix compared to that of the Alg hydrogel.					
30447366	2	30	theme	injectable	330:339	arg1	materials					356:364	injectable wound dressing materials	330:364	injectable wound dressing materials	330:364	Alginate (Alg) is an excellent candidate for injectable wound dressing materials because it can form a gel in a mild environment.					
30447366	3	31	theme	composite	477:485	arg1	hydrogel					487:494	an injectable nano composite hydrogel	458:494	an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles	458:564	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	0	32	theme	alginate	34:41	arg1	hydrogel					57:64	injectable alginate nanocomposite hydrogel	23:64	injectable alginate nanocomposite hydrogel	23:64	Fluorine-ion-releasing injectable alginate nanocomposite hydrogel for enhanced bioactivity and antibacterial property.					
30447366	5	33	theme	formation	962:970	arg1	test					972:975	colony formation test	955:975	colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel	955:1063	The antibacterial property of the composite hydrogel against E. coli and S. aureus was confirmed through colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel.					
30447366	1	34	dep	cell	172:175	arg1	migration					195:203	migration	195:203	migration	195:203	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	1	34	dep	cell	172:175	arg1	proliferation					177:189	proliferation	177:189	proliferation	177:189	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	4	35	dep	proliferation	792:804	arg1	the					788:790	the	788:790	the	788:790	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	7	36	theme	hydrogel	1325:1332	arg1	material					1375:1382	a bioactive injectable wound-dressing material	1337:1382	a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing	1337:1504	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	7	36	theme	hydrogel	1325:1332	arg1	potential					1293:1301	the potential	1289:1301	the potential of this nanocomposite hydrogel	1289:1332	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	3	37	theme	injectable	461:470	arg1	hydrogel					487:494	an injectable nano composite hydrogel	458:494	an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles	458:564	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	3	38	theme	property	448:455	arg1	advantage					422:430	advantage	422:430	advantage of its gelation property	422:455	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	3	39	theme	in-situ	586:592	arg1	process					608:614	in-situ precipitation process	586:614	in-situ precipitation process	586:614	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	5	40	theme	hydrogel	894:901	arg1	property					868:875	The antibacterial property	850:875	The antibacterial property of the composite hydrogel against E. coli and S. aureus	850:931	The antibacterial property of the composite hydrogel against E. coli and S. aureus was confirmed through colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel.					
30447366	4	41	theme	cells	834:838	arg1	migration					810:818	migration	810:818	migration	810:818	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	4	41	theme	cells	834:838	arg1	proliferation					792:804	proliferation	792:804	proliferation	792:804	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	3	42	theme	nano	472:475	arg1	hydrogel					487:494	an injectable nano composite hydrogel	458:494	an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles	458:564	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	4	43	theme	ion	654:656	arg1	ion					654:656	released fluorine (F-) ion	631:656	released fluorine (F-) ion from the nanocomposite hydrogel	631:688	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	4	43	theme	ion	654:656	arg1	amount					621:626	The amount	617:626	The amount of released fluorine (F-) ion from the nanocomposite hydrogel	617:688	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	3	44	theme	precipitation	594:606	arg1	process					608:614	in-situ precipitation process	586:614	in-situ precipitation process	586:614	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	1	45	theme	moist	137:141	arg1	environment					143:153	a moist environment	135:153	a moist environment	135:153	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	0	46	theme	enhanced	70:77	arg1	bioactivity					79:89	enhanced bioactivity	70:89	enhanced bioactivity	70:89	Fluorine-ion-releasing injectable alginate nanocomposite hydrogel for enhanced bioactivity and antibacterial property.					
30447366	6	47	theme	matrix	1220:1225	arg1	deposition					1188:1197	the deposition	1184:1197	the deposition of the extracellular matrix	1184:1225	The in vivo results based on a full-thickness wound model showed that the nanocomposite hydrogel effectively enhanced the deposition of the extracellular matrix compared to that of the Alg hydrogel.					
30447366	7	48	with	material	1375:1382	arg1	ability					1393:1399	the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing	1389:1504	the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing	1389:1504	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	1	49	dep	together	205:212	arg1	with					214:217	with	214:217	with	214:217	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	7	50	theme	nanocomposite	1311:1323	arg1	hydrogel					1325:1332	this nanocomposite hydrogel	1306:1332	this nanocomposite hydrogel	1306:1332	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	1	51	theme	environment	143:153	arg1	creation					123:130	The creation	119:130	The creation of a moist environment	119:153	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	1	51	theme	environment	143:153	arg1	promotion					159:167	promotion	159:167	promotion of cell proliferation and migration	159:203	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	1	51	theme	environment	143:153	arg1	critical					246:253	critical	246:253	critical	246:253	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	4	52	theme	nanocomposite	667:679	arg1	hydrogel					681:688	the nanocomposite hydrogel	663:688	the nanocomposite hydrogel	663:688	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	5	53	theme	colonies	1007:1014	arg1	number					987:992	the number	983:992	the number of bacterial colonies	983:1014	The antibacterial property of the composite hydrogel against E. coli and S. aureus was confirmed through colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel.					
30447366	5	54	theme	Alg	1052:1054	arg1	hydrogel					1056:1063	Alg hydrogel	1052:1063	Alg hydrogel	1052:1063	The antibacterial property of the composite hydrogel against E. coli and S. aureus was confirmed through colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel.					
30447366	4	55	theme	composite	740:748	arg1	hydrogel					750:757	the composite hydrogel	736:757	the composite hydrogel	736:757	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	5	56	theme	bacterial	997:1005	arg1	colonies					1007:1014	bacterial colonies	997:1014	bacterial colonies	997:1014	The antibacterial property of the composite hydrogel against E. coli and S. aureus was confirmed through colony formation test where the number of bacterial colonies significantly decreased compared to Alg hydrogel.					
30447366	7	57	theme	bacterial	1412:1420	arg1	growth					1422:1427	bacterial growth	1412:1427	bacterial growth	1412:1427	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	1	58	theme	cell	172:175	arg1	creation					123:130	The creation	119:130	The creation of a moist environment	119:153	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	1	58	theme	cell	172:175	arg1	promotion					159:167	promotion	159:167	promotion of cell proliferation and migration	159:203	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	1	58	theme	cell	172:175	arg1	critical					246:253	critical	246:253	critical	246:253	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	7	59	theme	wound-dressing	1360:1373	arg1	material					1375:1382	a bioactive injectable wound-dressing material	1337:1382	a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing	1337:1504	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	7	59	theme	wound-dressing	1360:1373	arg1	potential					1293:1301	the potential	1289:1301	the potential of this nanocomposite hydrogel	1289:1332	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	1	60	theme	wound-healing	262:274	arg1	process					276:282	the wound-healing process	258:282	the wound-healing process	258:282	The creation of a moist environment and promotion of cell proliferation and migration together with antibacterial property are critical to the wound-healing process.					
30447366	0	61	theme	antibacterial	95:107	arg1	property					109:116	antibacterial property	95:116	antibacterial property	95:116	Fluorine-ion-releasing injectable alginate nanocomposite hydrogel for enhanced bioactivity and antibacterial property.					
30447366	4	62	theme	fibroblast	823:832	arg1	cells					834:838	fibroblast cells	823:838	fibroblast cells	823:838	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	4	63	theme	F-	650:651	arg1	ion					654:656	released fluorine (F-) ion	631:656	released fluorine (F-) ion from the nanocomposite hydrogel	631:688	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	3	64	theme	calcium	532:538	arg1	particles					556:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	7	65	theme	bioactive	1339:1347	arg1	material					1375:1382	a bioactive injectable wound-dressing material	1337:1382	a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing	1337:1504	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	7	65	theme	bioactive	1339:1347	arg1	potential					1293:1301	the potential	1289:1301	the potential of this nanocomposite hydrogel	1289:1332	This study demonstrates the potential of this nanocomposite hydrogel as a bioactive injectable wound-dressing material with the ability to inhibit bacterial growth and stimulate cell proliferation and migration for accelerated wound healing.					
30447366	4	66	theme	fluorine	640:647	arg1	ion					654:656	released fluorine (F-) ion	631:656	released fluorine (F-) ion from the nanocomposite hydrogel	631:688	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
30447366	3	67	theme	fluoride	540:547	arg1	particles					556:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	nano-sized (about 90 nm) calcium fluoride (CaF2) particles	507:564	Taking advantage of its gelation property, an injectable nano composite hydrogel containing nano-sized (about 90 nm) calcium fluoride (CaF2) particles was developed using in-situ precipitation process.					
30447366	4	68	theme	released	631:638	arg1	ion					654:656	released fluorine (F-) ion	631:656	released fluorine (F-) ion from the nanocomposite hydrogel	631:688	The amount of released fluorine (F-) ion from the nanocomposite hydrogel increased with increasing CaF2 content inside the composite hydrogel and the ions stimulated both the proliferation and migration of fibroblast cells in vitro.					
31887376	0	0	theme	Tilapia	69:75	arg1	performance					11:21	growth performance	4:21	growth performance	4:21	The growth performance, body composition and nonspecific immunity of Tilapia (Oreochromis niloticus) affected by chitosan.					
31887376	0	0	theme	Tilapia	69:75	arg1	composition					29:39	body composition	24:39	body composition	24:39	The growth performance, body composition and nonspecific immunity of Tilapia (Oreochromis niloticus) affected by chitosan.					
31887376	0	0	theme	Tilapia	69:75	arg1	immunity					57:64	nonspecific immunity	45:64	nonspecific immunity	45:64	The growth performance, body composition and nonspecific immunity of Tilapia (Oreochromis niloticus) affected by chitosan.					
31887376	1	1	theme	study	139:143	arg1	aim					127:129	The aim	123:129	The aim of this study	123:143	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	1	2	theme	body	223:226	arg1	composition					228:238	body composition	223:238	body composition	223:238	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	4	3	theme	chitosan	875:882	arg1	level					866:870	a high level	859:870	a high level of chitosan (8 g kg-1)	859:893	However, a high level of chitosan (8 g kg-1) decreased its efficiency compared to moderate level of chitosan (4 g kg-1).					
31887376	3	4	theme	disease	590:596	arg1	ability					609:615	disease resistance ability	590:615	disease resistance ability	590:615	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	4	theme	disease	590:596	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	5	theme	weight	459:464	arg1	gain					466:469	body weight gain	454:469	body weight gain	454:469	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	5	theme	weight	459:464	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	6	dep	chitosan	435:442	arg1	increased					444:452	increased	444:452	increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila	444:644	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	6	dep	chitosan	435:442	arg1	decreased					650:658	decreased	650:658	decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group	650:847	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	7	theme	aspartate	740:748	arg1	aminotransferase					750:765	aspartate aminotransferase	740:765	aspartate aminotransferase	740:765	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	7	theme	aspartate	740:748	arg1	levels					682:687	hepatopancrease lipid levels	660:687	hepatopancrease lipid levels	660:687	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	0	8	theme	Oreochromis	78:88	arg1	Tilapia					69:75	Tilapia	69:75	Tilapia (Oreochromis niloticus)	69:99	The growth performance, body composition and nonspecific immunity of Tilapia (Oreochromis niloticus) affected by chitosan.					
31887376	0	8	theme	Oreochromis	78:88	arg1	niloticus					90:98	Oreochromis niloticus	78:98	Oreochromis niloticus	78:98	The growth performance, body composition and nonspecific immunity of Tilapia (Oreochromis niloticus) affected by chitosan.					
31887376	4	9	theme	chitosan	950:957	arg1	level					941:945	moderate level	932:945	moderate level of chitosan (4 g kg-1)	932:968	However, a high level of chitosan (8 g kg-1) decreased its efficiency compared to moderate level of chitosan (4 g kg-1).					
31887376	3	10	theme	body	454:457	arg1	gain					466:469	body weight gain	454:469	body weight gain	454:469	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	10	theme	body	454:457	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	11	theme	control	835:841	arg1	group					843:847	the control group	831:847	the control group	831:847	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	0	12	theme	growth	4:9	arg1	performance					11:21	growth performance	4:21	growth performance	4:21	The growth performance, body composition and nonspecific immunity of Tilapia (Oreochromis niloticus) affected by chitosan.					
31887376	3	13	theme	hepatopancrease	660:674	arg1	triacylglycerol					723:737	plasma triacylglycerol	716:737	plasma triacylglycerol	716:737	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	13	theme	hepatopancrease	660:674	arg1	aminotransferase					750:765	aspartate aminotransferase	740:765	aspartate aminotransferase	740:765	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	13	theme	hepatopancrease	660:674	arg1	aminotransferase					779:794	alanine aminotransferase	771:794	alanine aminotransferase	771:794	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	13	theme	hepatopancrease	660:674	arg1	levels					682:687	hepatopancrease lipid levels	660:687	hepatopancrease lipid levels	660:687	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	13	theme	hepatopancrease	660:674	arg1	cholesterol					703:713	plasma total cholesterol	690:713	plasma total cholesterol	690:713	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	14	theme	lipid	676:680	arg1	triacylglycerol					723:737	plasma triacylglycerol	716:737	plasma triacylglycerol	716:737	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	14	theme	lipid	676:680	arg1	aminotransferase					750:765	aspartate aminotransferase	740:765	aspartate aminotransferase	740:765	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	14	theme	lipid	676:680	arg1	aminotransferase					779:794	alanine aminotransferase	771:794	alanine aminotransferase	771:794	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	14	theme	lipid	676:680	arg1	levels					682:687	hepatopancrease lipid levels	660:687	hepatopancrease lipid levels	660:687	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	14	theme	lipid	676:680	arg1	cholesterol					703:713	plasma total cholesterol	690:713	plasma total cholesterol	690:713	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	15	theme	conversion	477:486	arg1	rate					510:513	specific growth rate	494:513	specific growth rate	494:513	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	15	theme	conversion	477:486	arg1	activity					551:558	superoxide dismutase activity	530:558	superoxide dismutase activity	530:558	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	15	theme	conversion	477:486	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	15	theme	conversion	477:486	arg1	ability					609:615	disease resistance ability	590:615	disease resistance ability	590:615	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	15	theme	conversion	477:486	arg1	protein					521:527	body protein	516:527	body protein	516:527	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	15	theme	conversion	477:486	arg1	activity					570:577	catalase activity	561:577	catalase activity	561:577	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	15	theme	conversion	477:486	arg1	gain					466:469	body weight gain	454:469	body weight gain	454:469	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	15	theme	conversion	477:486	arg1	lysozyme					580:587	lysozyme	580:587	lysozyme	580:587	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	1	16	theme	non-specific	244:255	arg1	immunity					257:264	non-specific immunity	244:264	non-specific immunity	244:264	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	5	17	theme	disease	1065:1071	arg1	resistance					1073:1082	their disease resistance	1059:1082	their disease resistance against A. hydrophila	1059:1104	The results demonstrated that chitosan could promote the growth of tilapias and improve their disease resistance against A. hydrophila.					
31887376	3	18	theme	growth	503:508	arg1	rate					510:513	specific growth rate	494:513	specific growth rate	494:513	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	18	theme	growth	503:508	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	4	19	theme	high	861:864	arg1	level					866:870	a high level	859:870	a high level of chitosan (8 g kg-1)	859:893	However, a high level of chitosan (8 g kg-1) decreased its efficiency compared to moderate level of chitosan (4 g kg-1).					
31887376	3	20	theme	feed	472:475	arg1	rate					510:513	specific growth rate	494:513	specific growth rate	494:513	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	20	theme	feed	472:475	arg1	activity					551:558	superoxide dismutase activity	530:558	superoxide dismutase activity	530:558	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	20	theme	feed	472:475	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	20	theme	feed	472:475	arg1	ability					609:615	disease resistance ability	590:615	disease resistance ability	590:615	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	20	theme	feed	472:475	arg1	protein					521:527	body protein	516:527	body protein	516:527	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	20	theme	feed	472:475	arg1	activity					570:577	catalase activity	561:577	catalase activity	561:577	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	20	theme	feed	472:475	arg1	gain					466:469	body weight gain	454:469	body weight gain	454:469	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	20	theme	feed	472:475	arg1	lysozyme					580:587	lysozyme	580:587	lysozyme	580:587	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	1	21	theme	tilapia	269:275	arg1	performance					210:220	growth performance	203:220	growth performance	203:220	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	1	21	theme	tilapia	269:275	arg1	composition					228:238	body composition	223:238	body composition	223:238	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	1	21	theme	tilapia	269:275	arg1	immunity					257:264	non-specific immunity	244:264	non-specific immunity	244:264	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	1	22	theme	dietary	179:185	arg1	chitosan					187:194	dietary chitosan	179:194	dietary chitosan	179:194	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	2	23	dep	test	367:370	arg1	feeds					372:376	feeds	372:376	feeds	372:376	Chitosan were added to the basic diet to formulate five kinds of test feeds (0, 2, 4, 6 and 8 g kg-1).					
31887376	2	24	theme	test	367:370	arg1	kinds					358:362	five kinds	353:362	five kinds of test feeds (0, 2, 4, 6 and 8 g kg-1)	353:402	Chitosan were added to the basic diet to formulate five kinds of test feeds (0, 2, 4, 6 and 8 g kg-1).					
31887376	0	25	theme	body	24:27	arg1	composition					29:39	body composition	24:39	body composition	24:39	The growth performance, body composition and nonspecific immunity of Tilapia (Oreochromis niloticus) affected by chitosan.					
31887376	3	26	theme	total	697:701	arg1	levels					682:687	hepatopancrease lipid levels	660:687	hepatopancrease lipid levels	660:687	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	26	theme	total	697:701	arg1	cholesterol					703:713	plasma total cholesterol	690:713	plasma total cholesterol	690:713	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	27	theme	alanine	771:777	arg1	aminotransferase					779:794	alanine aminotransferase	771:794	alanine aminotransferase	771:794	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	27	theme	alanine	771:777	arg1	levels					682:687	hepatopancrease lipid levels	660:687	hepatopancrease lipid levels	660:687	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	28	theme	superoxide	530:539	arg1	activity					551:558	superoxide dismutase activity	530:558	superoxide dismutase activity	530:558	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	28	theme	superoxide	530:539	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	1	29	theme	chitosan	187:194	arg1	effects					168:174	the effects	164:174	the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus)	164:299	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	0	30	theme	nonspecific	45:55	arg1	immunity					57:64	nonspecific immunity	45:64	nonspecific immunity	45:64	The growth performance, body composition and nonspecific immunity of Tilapia (Oreochromis niloticus) affected by chitosan.					
31887376	1	31	theme	Oreochromis	278:288	arg1	tilapia					269:275	tilapia	269:275	tilapia (Oreochromis niloticus)	269:299	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	1	31	theme	Oreochromis	278:288	arg1	niloticus					290:298	Oreochromis niloticus	278:298	Oreochromis niloticus	278:298	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	3	32	theme	dismutase	541:549	arg1	activity					551:558	superoxide dismutase activity	530:558	superoxide dismutase activity	530:558	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	32	theme	dismutase	541:549	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	4	33	theme	moderate	932:939	arg1	level					941:945	moderate level	932:945	moderate level of chitosan (4 g kg-1)	932:968	However, a high level of chitosan (8 g kg-1) decreased its efficiency compared to moderate level of chitosan (4 g kg-1).					
31887376	5	34	theme	tilapias	1038:1045	arg1	growth					1028:1033	the growth	1024:1033	the growth of tilapias	1024:1045	The results demonstrated that chitosan could promote the growth of tilapias and improve their disease resistance against A. hydrophila.					
31887376	3	35	theme	specific	494:501	arg1	rate					510:513	specific growth rate	494:513	specific growth rate	494:513	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	35	theme	specific	494:501	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	1	36	from	effects	168:174	arg1	performance					210:220	growth performance	203:220	growth performance	203:220	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	1	36	from	effects	168:174	arg1	composition					228:238	body composition	223:238	body composition	223:238	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	1	36	from	effects	168:174	arg1	immunity					257:264	non-specific immunity	244:264	non-specific immunity	244:264	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	3	37	theme	tilapias	799:806	arg1	aminotransferase					750:765	aspartate aminotransferase	740:765	aspartate aminotransferase	740:765	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	37	theme	tilapias	799:806	arg1	aminotransferase					779:794	alanine aminotransferase	771:794	alanine aminotransferase	771:794	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	37	theme	tilapias	799:806	arg1	levels					682:687	hepatopancrease lipid levels	660:687	hepatopancrease lipid levels	660:687	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	38	theme	plasma	690:695	arg1	levels					682:687	hepatopancrease lipid levels	660:687	hepatopancrease lipid levels	660:687	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	38	theme	plasma	690:695	arg1	cholesterol					703:713	plasma total cholesterol	690:713	plasma total cholesterol	690:713	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	39	theme	catalase	561:568	arg1	activity					570:577	catalase activity	561:577	catalase activity	561:577	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	39	theme	catalase	561:568	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	0	40	dep	performance	11:21	arg1	The					0:2	The	0:2	The	0:2	The growth performance, body composition and nonspecific immunity of Tilapia (Oreochromis niloticus) affected by chitosan.					
31887376	3	41	theme	4 g kg-1	426:433	arg1	chitosan					435:442	4 g kg-1 chitosan	426:442	4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group	426:847	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	1	42	theme	growth	203:208	arg1	performance					210:220	growth performance	203:220	growth performance	203:220	The aim of this study was to investigate the effects of dietary chitosan on the growth performance, body composition and non-specific immunity of tilapia (Oreochromis niloticus).					
31887376	3	43	theme	resistance	598:607	arg1	ability					609:615	disease resistance ability	590:615	disease resistance ability	590:615	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	43	theme	resistance	598:607	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	44	theme	body	516:519	arg1	protein					521:527	body protein	516:527	body protein	516:527	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	44	theme	body	516:519	arg1	rate					488:491	feed conversion rate	472:491	feed conversion rate	472:491	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	2	45	theme	basic	329:333	arg1	diet					335:338	the basic diet to formulate five kinds of test feeds (0, 2, 4, 6 and 8 g kg-1)	325:402	the basic diet to formulate five kinds of test feeds (0, 2, 4, 6 and 8 g kg-1)	325:402	Chitosan were added to the basic diet to formulate five kinds of test feeds (0, 2, 4, 6 and 8 g kg-1).					
31887376	3	46	theme	plasma	716:721	arg1	triacylglycerol					723:737	plasma triacylglycerol	716:737	plasma triacylglycerol	716:737	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
31887376	3	46	theme	plasma	716:721	arg1	levels					682:687	hepatopancrease lipid levels	660:687	hepatopancrease lipid levels	660:687	The diets containing 4 g kg-1 chitosan increased body weight gain, feed conversion rate, specific growth rate, body protein, superoxide dismutase activity, catalase activity, lysozyme, disease resistance ability against Aeromonas hydrophila and decreased hepatopancrease lipid levels, plasma total cholesterol, plasma triacylglycerol, aspartate aminotransferase and alanine aminotransferase of tilapias compared with those of the control group.					
30808727	4	0	theme	label-free	994:1003	arg1	spectrometry					1024:1035	label-free, quantitative mass spectrometry	994:1035	label-free, quantitative mass spectrometry	994:1035	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	4	1	theme	activation	680:689	arg1	status					691:696	the activation status	676:696	the activation status of the phagocyte	676:713	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	4	1	theme	activation	680:689	arg1	able					762:765	able	762:765	able	762:765	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	4	2	theme	DC	868:869	arg1	phagosomes					871:880	DC phagosomes	868:880	DC phagosomes	868:880	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	8	3	theme	maturation	1828:1837	arg1	duration					1806:1813	the duration	1802:1813	the duration of phagosome maturation	1802:1837	Hierarchical clustering of phagosomal proteins demonstrated that their acquisition to DC phagosomes is not only dependent on the duration of phagosome maturation but also on the activation state of DCs.					
30808727	3	4	theme	DCs	500:502	arg1	proteome					488:495	The phagosomal proteome	473:495	The phagosomal proteome of DCs	473:502	The phagosomal proteome of DCs is only partially studied and is highly dynamic as it changes during phagosome maturation, when phagosomes sequentially interact with endosomes and lysosomes.					
30808727	3	4	theme	DCs	500:502	arg1	dynamic					544:550	dynamic	544:550	dynamic	544:550	The phagosomal proteome of DCs is only partially studied and is highly dynamic as it changes during phagosome maturation, when phagosomes sequentially interact with endosomes and lysosomes.					
30808727	8	5	theme	phagosomal	1704:1713	arg1	proteins					1715:1722	phagosomal proteins	1704:1722	phagosomal proteins	1704:1722	Hierarchical clustering of phagosomal proteins demonstrated that their acquisition to DC phagosomes is not only dependent on the duration of phagosome maturation but also on the activation state of DCs.					
30808727	6	6	theme	proteins	1371:1378	arg1	recruitment					1356:1366	an increased recruitment	1343:1366	an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin	1343:1489	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	0	7	from	Changes	15:21	arg1	Response					72:79	Response	72:79	Response to LPS Stimulation	72:98	Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.					
30808727	0	7	from	Changes	15:21	arg1	Cells					63:67	Dendritic Cells	53:67	Dendritic Cells	53:67	Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.					
30808727	9	8	theme	comprehensive	1907:1919	arg1	overview					1921:1928	a comprehensive overview	1905:1928	a comprehensive overview	1905:1928	Thus, our study provides a comprehensive overview of how DCs alter their phagosome composition in response to LPS, which has profound impact on the initiation of efficient immune responses.					
30808727	1	9	theme	foreign	235:241	arg1	material					243:250	foreign material	235:250	foreign material	235:250	Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes.					
30808727	5	10	theme	proteins	1171:1178	arg1	recruitment					1156:1166	decreased recruitment	1146:1166	decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7	1146:1288	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	11	theme	latex	1056:1060	arg1	system					1100:1105	phagocytosis model system	1081:1105	phagocytosis model system	1081:1105	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	11	theme	latex	1056:1060	arg1	beads					1062:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	6	12	theme	molecules	1453:1461	arg1	tapasin					1483:1489	tapasin	1483:1489	tapasin	1483:1489	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	6	12	theme	molecules	1453:1461	arg1	proteasome					1468:1477	the proteasome	1464:1477	the proteasome	1464:1477	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	6	12	theme	molecules	1453:1461	arg1	subunits					1435:1442	different subunits	1425:1442	different subunits of MHC I molecules	1425:1461	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	5	13	theme	vacuolar	1238:1245	arg1	ATPase					1254:1259	the vacuolar proton ATPase	1234:1259	the vacuolar proton ATPase	1234:1259	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	0	14	theme	LPS	84:86	arg1	Stimulation					88:98	LPS Stimulation	84:98	LPS Stimulation	84:98	Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.					
30808727	4	15	theme	phagosomes	871:880	arg1	proteome					856:863	the proteome	852:863	the proteome of DC phagosomes	852:880	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	8	16	theme	activation	1855:1864	arg1	state					1866:1870	the activation state	1851:1870	the activation state of DCs	1851:1877	Hierarchical clustering of phagosomal proteins demonstrated that their acquisition to DC phagosomes is not only dependent on the duration of phagosome maturation but also on the activation state of DCs.					
30808727	9	17	dep	LPS	1990:1992	arg1	response					1978:1985	response	1978:1985	response	1978:1985	Thus, our study provides a comprehensive overview of how DCs alter their phagosome composition in response to LPS, which has profound impact on the initiation of efficient immune responses.					
30808727	5	18	theme	proton	1247:1252	arg1	ATPase					1254:1259	the vacuolar proton ATPase	1234:1259	the vacuolar proton ATPase	1234:1259	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	6	19	theme	increased	1346:1354	arg1	recruitment					1356:1366	an increased recruitment	1343:1366	an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin	1343:1489	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	5	20	theme	LPS-treated	1125:1135	arg1	DCs					1137:1139	LPS-treated DCs	1125:1139	LPS-treated DCs	1125:1139	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	4	21	theme	mass	1019:1022	arg1	spectrometry					1024:1035	label-free, quantitative mass spectrometry	994:1035	label-free, quantitative mass spectrometry	994:1035	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	6	22	theme	different	1425:1433	arg1	tapasin					1483:1489	tapasin	1483:1489	tapasin	1483:1489	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	6	22	theme	different	1425:1433	arg1	proteasome					1468:1477	the proteasome	1464:1477	the proteasome	1464:1477	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	6	22	theme	different	1425:1433	arg1	subunits					1435:1442	different subunits	1425:1442	different subunits of MHC I molecules	1425:1461	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	6	23	from	increase	1516:1523	arg1	efficacy					1547:1554	cross-presentation efficacy	1528:1554	cross-presentation efficacy	1528:1554	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	6	23	from	increase	1516:1523	arg1	cells					1565:1569	those cells	1559:1569	those cells	1559:1569	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	6	24	theme	MHC	1447:1449	arg1	molecules					1453:1461	MHC I molecules	1447:1461	MHC I molecules	1447:1461	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	8	25	theme	DC	1763:1764	arg1	phagosomes					1766:1775	DC phagosomes	1763:1775	DC phagosomes	1763:1775	Hierarchical clustering of phagosomal proteins demonstrated that their acquisition to DC phagosomes is not only dependent on the duration of phagosome maturation but also on the activation state of DCs.					
30808727	1	26	theme	innate	160:165	arg1	sensing					167:173	innate sensing	160:173	innate sensing	160:173	Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes.					
30808727	6	27	theme	I	1451:1451	arg1	molecules					1453:1461	MHC I molecules	1447:1461	MHC I molecules	1447:1461	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	5	28	used	used	1073:1076	arg2	beads					1062:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	28	used	used	1073:1076	arg2	system					1100:1105	phagocytosis model system	1081:1105	phagocytosis model system	1081:1105	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	4	29	theme	proteome	856:863	arg1	changes					841:847	spatiotemporal changes	826:847	spatiotemporal changes of the proteome of DC phagosomes	826:880	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	9	30	theme	immune	2052:2057	arg1	responses					2059:2067	efficient immune responses	2042:2067	efficient immune responses	2042:2067	Thus, our study provides a comprehensive overview of how DCs alter their phagosome composition in response to LPS, which has profound impact on the initiation of efficient immune responses.					
30808727	4	31	theme	phagosomal	732:741	arg1	composition					743:753	the phagosomal composition	728:753	the phagosomal composition	728:753	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	6	32	theme	cross-presentation	1528:1545	arg1	efficacy					1547:1554	cross-presentation efficacy	1528:1554	cross-presentation efficacy	1528:1554	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	0	33	theme	Spatiotemporal	0:13	arg1	Changes					15:21	Spatiotemporal Changes	0:21	Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.	0:99	Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.					
30808727	7	34	theme	phagosomal	1655:1664	arg1	functions					1666:1674	phagosomal functions	1655:1674	phagosomal functions	1655:1674	Further, several proteins were identified that were not previously associated with phagosomal functions.					
30808727	9	35	theme	responses	2059:2067	arg1	initiation					2028:2037	the initiation	2024:2037	the initiation of efficient immune responses	2024:2067	Thus, our study provides a comprehensive overview of how DCs alter their phagosome composition in response to LPS, which has profound impact on the initiation of efficient immune responses.					
30808727	2	36	theme	T	427:427	arg1	cells					429:433	T cells	427:433	T cells initiating adaptive immune responses	427:470	These intracellular compartments are equipped to generate antigenic peptides that serve as source for antigen presentation to T cells initiating adaptive immune responses.					
30808727	8	37	theme	DCs	1875:1877	arg1	state					1866:1870	the activation state	1851:1870	the activation state of DCs	1851:1877	Hierarchical clustering of phagosomal proteins demonstrated that their acquisition to DC phagosomes is not only dependent on the duration of phagosome maturation but also on the activation state of DCs.					
30808727	9	38	theme	phagosome	1953:1961	arg1	composition					1963:1973	their phagosome composition	1947:1973	their phagosome composition	1947:1973	Thus, our study provides a comprehensive overview of how DCs alter their phagosome composition in response to LPS, which has profound impact on the initiation of efficient immune responses.					
30808727	1	39	theme	pathogenic	261:270	arg1	bacteria					272:279	pathogenic bacteria	261:279	pathogenic bacteria	261:279	Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes.					
30808727	4	40	theme	resting	919:925	arg1	DCs					987:989	resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs	919:989	resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs	919:989	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	5	41	theme	model	1094:1098	arg1	system					1100:1105	phagocytosis model system	1081:1105	phagocytosis model system	1081:1105	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	41	theme	model	1094:1098	arg1	beads					1062:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	1	42	theme	Dendritic	101:109	arg1	DCs					118:120	DCs	118:120	DCs	118:120	Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes.					
30808727	1	42	theme	Dendritic	101:109	arg1	cells					111:115	Dendritic cells	101:115	Dendritic cells (DCs)	101:121	Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes.					
30808727	1	42	theme	Dendritic	101:109	arg1	phagocytes					140:149	professional phagocytes	127:149	professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes	127:298	Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes.					
30808727	6	43	theme	observed	1507:1514	arg1	increase					1516:1523	the observed increase	1503:1523	the observed increase in cross-presentation efficacy in those cells	1503:1569	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	2	44	theme	antigen	403:409	arg1	presentation					411:422	antigen presentation	403:422	antigen presentation to T cells initiating adaptive immune responses	403:470	These intracellular compartments are equipped to generate antigenic peptides that serve as source for antigen presentation to T cells initiating adaptive immune responses.					
30808727	0	45	theme	Proteome	41:48	arg1	Changes					15:21	Spatiotemporal Changes	0:21	Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.	0:99	Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.					
30808727	4	46	dep	shape	770:774	arg1	compared					910:917	compared	910:917	compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry	910:1035	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	4	46	dep	shape	770:774	arg1	determined					815:824	determined	815:824	determined spatiotemporal changes of the proteome of DC phagosomes during their maturation	815:904	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	0	47	theme	Phagosomal	30:39	arg1	Proteome					41:48	the Phagosomal Proteome	26:48	the Phagosomal Proteome	26:48	Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.					
30808727	4	48	link	marrow-derived	972:985	arg1	DCs					987:989	resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs	919:989	resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs	919:989	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	9	49	contain	has	2001:2003	arg2	impact					2014:2019	profound impact	2005:2019	profound impact	2005:2019	Thus, our study provides a comprehensive overview of how DCs alter their phagosome composition in response to LPS, which has profound impact on the initiation of efficient immune responses.					
30808727	9	49	contain	has	2001:2003	arg1	overview					1921:1928	a comprehensive overview	1905:1928	a comprehensive overview	1905:1928	Thus, our study provides a comprehensive overview of how DCs alter their phagosome composition in response to LPS, which has profound impact on the initiation of efficient immune responses.					
30808727	5	50	theme	Ovalbumin-coupled	1038:1054	arg1	system					1100:1105	phagocytosis model system	1081:1105	phagocytosis model system	1081:1105	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	50	theme	Ovalbumin-coupled	1038:1054	arg1	beads					1062:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	4	51	theme	-stimulated	955:965	arg1	DCs					987:989	resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs	919:989	resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs	919:989	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	0	52	theme	Dendritic	53:61	arg1	Cells					63:67	Dendritic Cells	53:67	Dendritic Cells	53:67	Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation.					
30808727	4	53	dep	label-free	994:1003	arg1	quantitative					1006:1017	quantitative	1006:1017	quantitative	1006:1017	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	4	54	theme	marrow-derived	972:985	arg1	DCs					987:989	resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs	919:989	resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs	919:989	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	4	55	theme	spatiotemporal	826:839	arg1	changes					841:847	spatiotemporal changes	826:847	spatiotemporal changes of the proteome of DC phagosomes	826:880	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	4	56	theme	phagocyte	705:713	arg1	status					691:696	the activation status	676:696	the activation status of the phagocyte	676:713	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	4	56	theme	phagocyte	705:713	arg1	able					762:765	able	762:765	able	762:765	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	2	57	theme	adaptive	446:453	arg1	responses					462:470	adaptive immune responses	446:470	adaptive immune responses	446:470	These intracellular compartments are equipped to generate antigenic peptides that serve as source for antigen presentation to T cells initiating adaptive immune responses.					
30808727	5	58	theme	phagosome	1192:1200	arg1	maturation					1202:1211	phagosome maturation	1192:1211	phagosome maturation	1192:1211	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	6	59	dep	subunits	1435:1442	arg1	e.g.					1420:1423	e.g.	1420:1423	e.g.	1420:1423	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	3	60	theme	phagosome	573:581	arg1	maturation					583:592	phagosome maturation	573:592	phagosome maturation	573:592	The phagosomal proteome of DCs is only partially studied and is highly dynamic as it changes during phagosome maturation, when phagosomes sequentially interact with endosomes and lysosomes.					
30808727	8	61	theme	Hierarchical	1677:1688	arg1	clustering					1690:1699	Hierarchical clustering	1677:1699	Hierarchical clustering of phagosomal proteins	1677:1722	Hierarchical clustering of phagosomal proteins demonstrated that their acquisition to DC phagosomes is not only dependent on the duration of phagosome maturation but also on the activation state of DCs.					
30808727	9	62	theme	efficient	2042:2050	arg1	responses					2059:2067	efficient immune responses	2042:2067	efficient immune responses	2042:2067	Thus, our study provides a comprehensive overview of how DCs alter their phagosome composition in response to LPS, which has profound impact on the initiation of efficient immune responses.					
30808727	2	63	theme	intracellular	307:319	arg1	compartments					321:332	These intracellular compartments	301:332	These intracellular compartments	301:332	These intracellular compartments are equipped to generate antigenic peptides that serve as source for antigen presentation to T cells initiating adaptive immune responses.					
30808727	5	64	theme	ATPase	1254:1259	arg1	RAB7					1285:1288	RAB7	1285:1288	RAB7	1285:1288	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	64	theme	ATPase	1254:1259	arg1	B					1272:1272	cathepsin B	1262:1272	cathepsin B	1262:1272	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	64	theme	ATPase	1254:1259	arg1	S					1278:1278	S	1278:1278	S	1278:1278	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	64	theme	ATPase	1254:1259	arg1	D					1275:1275	D	1275:1275	D	1275:1275	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	64	theme	ATPase	1254:1259	arg1	subunits					1222:1229	subunits	1222:1229	subunits of the vacuolar proton ATPase	1222:1259	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	4	65	theme	phagosomal	776:785	arg1	functions.In					787:798	phagosomal functions.In	776:798	phagosomal functions.In	776:798	In addition, the activation status of the phagocyte can modulate the phagosomal composition and is able to shape phagosomal functions.In this study, we determined spatiotemporal changes of the proteome of DC phagosomes during their maturation and compared resting and lipopolysaccharide (LPS)-stimulated bone marrow-derived DCs by label-free, quantitative mass spectrometry.					
30808727	8	66	theme	phagosome	1818:1826	arg1	maturation					1828:1837	phagosome maturation	1818:1837	phagosome maturation	1818:1837	Hierarchical clustering of phagosomal proteins demonstrated that their acquisition to DC phagosomes is not only dependent on the duration of phagosome maturation but also on the activation state of DCs.					
30808727	3	67	theme	phagosomal	477:486	arg1	proteome					488:495	The phagosomal proteome	473:495	The phagosomal proteome of DCs	473:502	The phagosomal proteome of DCs is only partially studied and is highly dynamic as it changes during phagosome maturation, when phagosomes sequentially interact with endosomes and lysosomes.					
30808727	3	67	theme	phagosomal	477:486	arg1	dynamic					544:550	dynamic	544:550	dynamic	544:550	The phagosomal proteome of DCs is only partially studied and is highly dynamic as it changes during phagosome maturation, when phagosomes sequentially interact with endosomes and lysosomes.					
30808727	7	68	theme	several	1581:1587	arg1	proteins					1589:1596	several proteins	1581:1596	several proteins	1581:1596	Further, several proteins were identified that were not previously associated with phagosomal functions.					
30808727	6	69	theme	antigen	1392:1398	arg1	cross-presentation					1400:1417	antigen cross-presentation	1392:1417	antigen cross-presentation	1392:1417	In contrast, those phagosomes were characterized by an increased recruitment of proteins involved in antigen cross-presentation, e.g. different subunits of MHC I molecules, the proteasome and tapasin, confirming the observed increase in cross-presentation efficacy in those cells.					
30808727	5	70	theme	phagocytosis	1081:1092	arg1	system					1100:1105	phagocytosis model system	1081:1105	phagocytosis model system	1081:1105	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	70	theme	phagocytosis	1081:1092	arg1	beads					1062:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads	1038:1066	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	2	71	theme	antigenic	359:367	arg1	peptides					369:376	antigenic peptides	359:376	antigenic peptides that serve as source for antigen presentation to T cells initiating adaptive immune responses	359:470	These intracellular compartments are equipped to generate antigenic peptides that serve as source for antigen presentation to T cells initiating adaptive immune responses.					
30808727	5	72	theme	cathepsin	1262:1270	arg1	subunits					1222:1229	subunits	1222:1229	subunits of the vacuolar proton ATPase	1222:1259	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	5	72	theme	cathepsin	1262:1270	arg1	B					1272:1272	cathepsin B	1262:1272	cathepsin B	1262:1272	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	2	73	theme	immune	455:460	arg1	responses					462:470	adaptive immune responses	446:470	adaptive immune responses	446:470	These intracellular compartments are equipped to generate antigenic peptides that serve as source for antigen presentation to T cells initiating adaptive immune responses.					
30808727	5	74	theme	decreased	1146:1154	arg1	recruitment					1156:1166	decreased recruitment	1146:1166	decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7	1146:1288	Ovalbumin-coupled latex beads were used as phagocytosis model system and revealed that LPS-treated DCs show decreased recruitment of proteins involved in phagosome maturation, such as subunits of the vacuolar proton ATPase, cathepsin B, D, S, and RAB7.					
30808727	9	75	theme	profound	2005:2012	arg1	impact					2014:2019	profound impact	2005:2019	profound impact	2005:2019	Thus, our study provides a comprehensive overview of how DCs alter their phagosome composition in response to LPS, which has profound impact on the initiation of efficient immune responses.					
30808727	1	76	theme	professional	127:138	arg1	cells					111:115	Dendritic cells	101:115	Dendritic cells (DCs)	101:121	Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes.					
30808727	1	76	theme	professional	127:138	arg1	phagocytes					140:149	professional phagocytes	127:149	professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes	127:298	Dendritic cells (DCs) are professional phagocytes that use innate sensing and phagocytosis to internalize and degrade self as well as foreign material, such as pathogenic bacteria, within phagosomes.					
30808727	8	77	theme	proteins	1715:1722	arg1	clustering					1690:1699	Hierarchical clustering	1677:1699	Hierarchical clustering of phagosomal proteins	1677:1722	Hierarchical clustering of phagosomal proteins demonstrated that their acquisition to DC phagosomes is not only dependent on the duration of phagosome maturation but also on the activation state of DCs.					
29479806	0	0	theme	new	72:74	arg1	material					87:94	a new bone graft material	70:94	a new bone graft material	70:94	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.					
29479806	7	1	used	used	782:785	arg2	It					775:776	It	775:776	It	775:776	It was used in combination with different proportions of nano-hydroxyapatite (n-HA), to develop new series of β-CS/n-HA nanocomposites.					
29479806	8	2	theme	70	976:977	arg1	%					978:978	%	978:978	%	978:978	Sample were obtained in a powder form with the ratio of 30 CH to 70% nHA.					
29479806	6	3	theme	β-type	682:687	arg1	chitosan					689:696	β-type chitosan	682:696	β-type chitosan	682:696	MATERIAL AND METHODS β-type chitosan was obtained through a modified procedure from squid pens (Loligo vulgaris).					
29479806	4	4	theme	physical	424:431	arg1	properties					448:457	the physical and mechanical properties	420:457	the physical and mechanical properties	420:457	Some of the physical and mechanical properties were examined by using (scanning electron microscope and transmission electron microscope).					
29479806	9	5	theme	adult	1050:1054	arg1	animals					1041:1047	the animals	1037:1047	the animals (adult rabbits)	1037:1063	The product was implanted in the femoral condyle of the animals (adult rabbits).					
29479806	9	5	theme	adult	1050:1054	arg1	rabbits					1056:1062	adult rabbits	1050:1062	adult rabbits	1050:1062	The product was implanted in the femoral condyle of the animals (adult rabbits).					
29479806	0	6	theme	graft	81:85	arg1	material					87:94	a new bone graft material	70:94	a new bone graft material	70:94	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.					
29479806	3	7	with	PURPOSE	337:343	arg1	percentages					386:396	different percentages	376:396	different percentages	376:396	PURPOSE β-Chitosan/n-HA composite with different percentages was prepared.					
29479806	11	8	theme	bone	1263:1266	arg1	growth					1268:1273	bone growth	1263:1273	bone growth	1263:1273	Histological examinations showed that the implant not only biological compatible but also its presence promotes and accelerate bone growth.					
29479806	13	9	theme	artificial	1516:1525	arg1	graft					1532:1536	artificial bone graft	1516:1536	artificial bone graft	1516:1536	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	12	10	theme	biodegradable	1322:1334	arg1	bone					1336:1339	biodegradable bone	1322:1339	biodegradable bone	1322:1339	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone substitute that not only enhance bone generation but also accelerate the formation of Haversian system.					
29479806	12	11	theme	bone	1374:1377	arg1	generation					1379:1388	bone generation	1374:1388	bone generation	1374:1388	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone substitute that not only enhance bone generation but also accelerate the formation of Haversian system.					
29479806	0	12	theme	bone	76:79	arg1	material					87:94	a new bone graft material	70:94	a new bone graft material	70:94	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.					
29479806	13	13	theme	30/70	1585:1589	arg1	ratio					1591:1595	30/70 ratio	1585:1595	30/70 ratio of β-CS/HPa	1585:1607	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	4	14	theme	mechanical	437:446	arg1	properties					448:457	the physical and mechanical properties	420:457	the physical and mechanical properties	420:457	Some of the physical and mechanical properties were examined by using (scanning electron microscope and transmission electron microscope).					
29479806	2	15	theme	ceramics	304:311	arg1	CTS					324:326	CTS	324:326	CTS	324:326	Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap.					
29479806	2	15	theme	ceramics	304:311	arg1	combinations					251:262	combinations	251:262	combinations	251:262	Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap.					
29479806	2	15	theme	ceramics	304:311	arg1	Hap					332:334	Hap	332:334	Hap	332:334	Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap.					
29479806	7	16	theme	nanocomposites	895:908	arg1	series					875:880	new series	871:880	new series of β-CS/n-HA nanocomposites	871:908	It was used in combination with different proportions of nano-hydroxyapatite (n-HA), to develop new series of β-CS/n-HA nanocomposites.					
29479806	7	17	theme	β-CS/n-HA	885:893	arg1	nanocomposites					895:908	β-CS/n-HA nanocomposites	885:908	β-CS/n-HA nanocomposites	885:908	It was used in combination with different proportions of nano-hydroxyapatite (n-HA), to develop new series of β-CS/n-HA nanocomposites.					
29479806	2	18	theme	bioactive	294:302	arg1	ceramics					304:311	bioactive ceramics	294:311	bioactive ceramics	294:311	Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap.					
29479806	6	19	theme	Loligo	757:762	arg1	pens					751:754	squid pens	745:754	squid pens (Loligo vulgaris)	745:772	MATERIAL AND METHODS β-type chitosan was obtained through a modified procedure from squid pens (Loligo vulgaris).					
29479806	6	19	theme	Loligo	757:762	arg1	vulgaris					764:771	Loligo vulgaris	757:771	Loligo vulgaris	757:771	MATERIAL AND METHODS β-type chitosan was obtained through a modified procedure from squid pens (Loligo vulgaris).					
29479806	12	20	theme	composite	1292:1300	arg1	30/70					1312:1316	30/70	1312:1316	30/70	1312:1316	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone substitute that not only enhance bone generation but also accelerate the formation of Haversian system.					
29479806	12	20	theme	composite	1292:1300	arg1	β-CS/HPa					1302:1309	The composite β-CS/HPa	1288:1309	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone	1276:1339	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone substitute that not only enhance bone generation but also accelerate the formation of Haversian system.					
29479806	3	21	theme	different	376:384	arg1	percentages					386:396	different percentages	376:396	different percentages	376:396	PURPOSE β-Chitosan/n-HA composite with different percentages was prepared.					
29479806	4	22	theme	electron	529:536	arg1	microscope					538:547	transmission electron microscope	516:547	transmission electron microscope	516:547	Some of the physical and mechanical properties were examined by using (scanning electron microscope and transmission electron microscope).					
29479806	8	23	with	form	944:947	arg1	ratio					958:962	the ratio	954:962	the ratio of 30 CH to 70% nHA	954:982	Sample were obtained in a powder form with the ratio of 30 CH to 70% nHA.					
29479806	13	24	theme	suitable	1615:1622	arg1	strength					1635:1642	suitable mechanical strength	1615:1642	suitable mechanical strength to be employed as a solid-shaped implants	1615:1684	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	10	25	theme	30/70	1129:1133	arg1	ratio					1120:1124	the weight ratio	1109:1124	the weight ratio of 30/70	1109:1133	RESULTS Compact strength was 13.05 MPa for the weight ratio of 30/70.					
29479806	4	26	theme	transmission	516:527	arg1	microscope					538:547	transmission electron microscope	516:547	transmission electron microscope	516:547	Some of the physical and mechanical properties were examined by using (scanning electron microscope and transmission electron microscope).					
29479806	13	27	theme	solid-shaped	1664:1675	arg1	implants					1677:1684	a solid-shaped implants	1662:1684	a solid-shaped implants	1662:1684	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	5	28	theme	bone	631:634	arg1	material					642:649	bone graft material	631:649	bone graft material	631:649	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done.					
29479806	13	29	theme	mechanical	1547:1556	arg1	properties					1558:1567	the mechanical properties	1543:1567	the mechanical properties	1543:1567	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	5	30	theme	Histological	551:562	arg1	evaluation					564:573	Histological evaluation	551:573	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material	551:649	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done.					
29479806	13	31	theme	bone	1527:1530	arg1	graft					1532:1536	artificial bone graft	1516:1536	artificial bone graft	1516:1536	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	0	32	theme	In	0:1	arg1	evaluation					8:17	In vivo evaluation	0:17	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.	0:95	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.					
29479806	5	33	theme	graft	636:640	arg1	material					642:649	bone graft material	631:649	bone graft material	631:649	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done.					
29479806	5	34	dep	in	578:579	arg1	vivo					581:584	vivo	581:584	vivo	581:584	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done.					
29479806	12	35	theme	system	1437:1442	arg1	formation					1414:1422	the formation	1410:1422	the formation of Haversian system	1410:1442	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone substitute that not only enhance bone generation but also accelerate the formation of Haversian system.					
29479806	10	36	theme	weight	1113:1118	arg1	ratio					1120:1124	the weight ratio	1109:1124	the weight ratio of 30/70	1109:1133	RESULTS Compact strength was 13.05 MPa for the weight ratio of 30/70.					
29479806	7	37	theme	new	871:873	arg1	series					875:880	new series	871:880	new series of β-CS/n-HA nanocomposites	871:908	It was used in combination with different proportions of nano-hydroxyapatite (n-HA), to develop new series of β-CS/n-HA nanocomposites.					
29479806	1	38	theme	bone	197:200	arg1	engineering					209:219	bone tissue engineering	197:219	bone tissue engineering	197:219	BACKGROUND Natural polymer composite materials are becoming increasingly important as scaffolds for bone tissue engineering.					
29479806	13	39	from	composite	1457:1465	arg1	form					1479:1482	a powder form	1470:1482	a powder form	1470:1482	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	11	40	theme	Histological	1136:1147	arg1	examinations					1149:1160	Histological examinations	1136:1160	Histological examinations	1136:1160	Histological examinations showed that the implant not only biological compatible but also its presence promotes and accelerate bone growth.					
29479806	1	41	theme	tissue	202:207	arg1	engineering					209:219	bone tissue engineering	197:219	bone tissue engineering	197:219	BACKGROUND Natural polymer composite materials are becoming increasingly important as scaffolds for bone tissue engineering.					
29479806	13	42	used	used	1448:1451	arg2	We					1445:1446	We	1445:1446	We	1445:1446	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	12	43	dep	CONCLUSIONS	1276:1286	arg1	30/70					1312:1316	30/70	1312:1316	30/70	1312:1316	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone substitute that not only enhance bone generation but also accelerate the formation of Haversian system.					
29479806	12	43	dep	CONCLUSIONS	1276:1286	arg1	β-CS/HPa					1302:1309	The composite β-CS/HPa	1288:1309	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone	1276:1339	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone substitute that not only enhance bone generation but also accelerate the formation of Haversian system.					
29479806	6	44	theme	modified	721:728	arg1	procedure					730:738	a modified procedure	719:738	a modified procedure	719:738	MATERIAL AND METHODS β-type chitosan was obtained through a modified procedure from squid pens (Loligo vulgaris).					
29479806	0	45	theme	β-CS/n-HA	22:30	arg1	evaluation					8:17	In vivo evaluation	0:17	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.	0:95	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.					
29479806	4	46	theme	electron	492:499	arg1	microscope					501:510	scanning electron microscope	483:510	scanning electron microscope	483:510	Some of the physical and mechanical properties were examined by using (scanning electron microscope and transmission electron microscope).					
29479806	12	47	theme	Haversian	1427:1435	arg1	system					1437:1442	Haversian system	1427:1442	Haversian system	1427:1442	CONCLUSIONS The composite β-CS/HPa (30/70) as biodegradable bone substitute that not only enhance bone generation but also accelerate the formation of Haversian system.					
29479806	5	48	theme	implantation	586:597	arg1	evaluation					564:573	Histological evaluation	551:573	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material	551:649	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done.					
29479806	13	49	theme	powder	1472:1477	arg1	form					1479:1482	a powder form	1470:1482	a powder form	1470:1482	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	0	50	theme	physical	47:54	arg1	properties					56:65	different physical properties	37:65	different physical properties as a new bone graft material	37:94	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.					
29479806	7	51	theme	nano-hydroxyapatite	832:850	arg1	proportions					817:827	different proportions	807:827	different proportions of nano-hydroxyapatite (n-HA)	807:857	It was used in combination with different proportions of nano-hydroxyapatite (n-HA), to develop new series of β-CS/n-HA nanocomposites.					
29479806	13	52	theme	β-CS/HPa	1600:1607	arg1	ratio					1591:1595	30/70 ratio	1585:1595	30/70 ratio of β-CS/HPa	1585:1607	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	4	53	theme	scanning	483:490	arg1	microscope					501:510	scanning electron microscope	483:510	scanning electron microscope	483:510	Some of the physical and mechanical properties were examined by using (scanning electron microscope and transmission electron microscope).					
29479806	0	54	with	evaluation	8:17	arg1	properties					56:65	different physical properties	37:65	different physical properties as a new bone graft material	37:94	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.					
29479806	1	55	theme	BACKGROUND	97:106	arg1	materials					134:142	BACKGROUND Natural polymer composite materials	97:142	BACKGROUND Natural polymer composite materials	97:142	BACKGROUND Natural polymer composite materials are becoming increasingly important as scaffolds for bone tissue engineering.					
29479806	0	56	theme	different	37:45	arg1	properties					56:65	different physical properties	37:65	different physical properties as a new bone graft material	37:94	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.					
29479806	7	57	theme	different	807:815	arg1	proportions					817:827	different proportions	807:827	different proportions of nano-hydroxyapatite (n-HA)	807:857	It was used in combination with different proportions of nano-hydroxyapatite (n-HA), to develop new series of β-CS/n-HA nanocomposites.					
29479806	2	58	theme	Composite	222:230	arg1	materials					232:240	Composite materials	222:240	Composite materials	222:240	Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap.					
29479806	5	59	theme	β-Chitosan/n-HA	602:616	arg1	composite					618:626	β-Chitosan/n-HA composite	602:626	β-Chitosan/n-HA composite	602:626	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done.					
29479806	1	60	theme	Natural	108:114	arg1	materials					134:142	BACKGROUND Natural polymer composite materials	97:142	BACKGROUND Natural polymer composite materials	97:142	BACKGROUND Natural polymer composite materials are becoming increasingly important as scaffolds for bone tissue engineering.					
29479806	9	61	theme	femoral	1018:1024	arg1	condyle					1026:1032	the femoral condyle	1014:1032	the femoral condyle of the animals (adult rabbits)	1014:1063	The product was implanted in the femoral condyle of the animals (adult rabbits).					
29479806	10	62	dep	RESULTS	1066:1072	arg1	MPa					1101:1103	13.05 MPa	1095:1103	13.05 MPa	1095:1103	RESULTS Compact strength was 13.05 MPa for the weight ratio of 30/70.					
29479806	6	63	dep	MATERIAL	661:668	arg1	chitosan					689:696	β-type chitosan	682:696	β-type chitosan	682:696	MATERIAL AND METHODS β-type chitosan was obtained through a modified procedure from squid pens (Loligo vulgaris).					
29479806	8	64	theme	%	978:978	arg1	nHA					980:982	70% nHA	976:982	70% nHA	976:982	Sample were obtained in a powder form with the ratio of 30 CH to 70% nHA.					
29479806	2	65	theme	polymers	281:288	arg1	CTS					324:326	CTS	324:326	CTS	324:326	Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap.					
29479806	2	65	theme	polymers	281:288	arg1	combinations					251:262	combinations	251:262	combinations	251:262	Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap.					
29479806	2	65	theme	polymers	281:288	arg1	Hap					332:334	Hap	332:334	Hap	332:334	Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap.					
29479806	5	66	theme	composite	618:626	arg1	implantation					586:597	in vivo implantation	578:597	in vivo implantation of β-Chitosan/n-HA composite	578:626	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done.					
29479806	1	67	theme	polymer	116:122	arg1	materials					134:142	BACKGROUND Natural polymer composite materials	97:142	BACKGROUND Natural polymer composite materials	97:142	BACKGROUND Natural polymer composite materials are becoming increasingly important as scaffolds for bone tissue engineering.					
29479806	7	68	with	combination	790:800	arg1	proportions					817:827	different proportions	807:827	different proportions of nano-hydroxyapatite (n-HA)	807:857	It was used in combination with different proportions of nano-hydroxyapatite (n-HA), to develop new series of β-CS/n-HA nanocomposites.					
29479806	13	69	theme	mechanical	1624:1633	arg1	strength					1635:1642	suitable mechanical strength	1615:1642	suitable mechanical strength to be employed as a solid-shaped implants	1615:1684	We used the composite in a powder form and examined its suitability as artificial bone graft; yet the mechanical properties have shown that 30/70 ratio of β-CS/HPa offer suitable mechanical strength to be employed as a solid-shaped implants.					
29479806	2	70	theme	biodegradable	267:279	arg1	polymers					281:288	biodegradable polymers	267:288	biodegradable polymers	267:288	Composite materials based on combinations of biodegradable polymers and bioactive ceramics, including CTS and Hap.					
29479806	1	71	theme	composite	124:132	arg1	materials					134:142	BACKGROUND Natural polymer composite materials	97:142	BACKGROUND Natural polymer composite materials	97:142	BACKGROUND Natural polymer composite materials are becoming increasingly important as scaffolds for bone tissue engineering.					
29479806	3	72	theme	β-Chitosan/n-HA	345:359	arg1	PURPOSE					337:343	PURPOSE β-Chitosan/n-HA composite	337:369	PURPOSE β-Chitosan/n-HA composite with different percentages	337:396	PURPOSE β-Chitosan/n-HA composite with different percentages was prepared.					
29479806	8	73	theme	powder	937:942	arg1	form					944:947	a powder form	935:947	a powder form with the ratio of 30 CH to 70% nHA	935:982	Sample were obtained in a powder form with the ratio of 30 CH to 70% nHA.					
29479806	8	74	theme	CH	970:971	arg1	ratio					958:962	the ratio	954:962	the ratio of 30 CH to 70% nHA	954:982	Sample were obtained in a powder form with the ratio of 30 CH to 70% nHA.					
29479806	6	75	theme	squid	745:749	arg1	pens					751:754	squid pens	745:754	squid pens (Loligo vulgaris)	745:772	MATERIAL AND METHODS β-type chitosan was obtained through a modified procedure from squid pens (Loligo vulgaris).					
29479806	6	75	theme	squid	745:749	arg1	vulgaris					764:771	Loligo vulgaris	757:771	Loligo vulgaris	757:771	MATERIAL AND METHODS β-type chitosan was obtained through a modified procedure from squid pens (Loligo vulgaris).					
29479806	3	76	theme	composite	361:369	arg1	PURPOSE					337:343	PURPOSE β-Chitosan/n-HA composite	337:369	PURPOSE β-Chitosan/n-HA composite with different percentages	337:396	PURPOSE β-Chitosan/n-HA composite with different percentages was prepared.					
29479806	10	77	theme	Compact	1074:1080	arg1	strength					1082:1089	Compact strength	1074:1089	Compact strength	1074:1089	RESULTS Compact strength was 13.05 MPa for the weight ratio of 30/70.					
29479806	0	78	dep	In	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	In vivo evaluation of β-CS/n-HA with different physical properties as a new bone graft material.					
29479806	5	79	theme	in	578:579	arg1	implantation					586:597	in vivo implantation	578:597	in vivo implantation of β-Chitosan/n-HA composite	578:626	Histological evaluation of in vivo implantation of β-Chitosan/n-HA composite as bone graft material was done.					
29479806	9	80	theme	animals	1041:1047	arg1	condyle					1026:1032	the femoral condyle	1014:1032	the femoral condyle of the animals (adult rabbits)	1014:1063	The product was implanted in the femoral condyle of the animals (adult rabbits).					
31338614	9	0	theme	plant-based	1855:1865	arg1	endoglucanases					1871:1884	novel plant-based GH9 endoglucanases	1849:1884	novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass	1849:1964	Results presented may aid the development of novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass.					
31338614	7	1	theme	Crystalline	1411:1421	arg1	substrate					1449:1457	the de facto substrate	1436:1457	the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology	1436:1578	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	7	1	theme	Crystalline	1411:1421	arg1	cellulose					1423:1431	Crystalline cellulose	1411:1431	Crystalline cellulose	1411:1431	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	6	2	theme	measurable	1109:1118	arg1	cavities					1120:1127	measurable cavities	1109:1127	measurable cavities	1109:1127	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	9	3	theme	endoglucanases	1871:1884	arg1	development					1834:1844	the development	1830:1844	the development of novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass	1830:1964	Results presented may aid the development of novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass.					
31338614	6	4	theme	acid	1196:1199	arg1	composition					1201:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	8	5	theme	cellulose	1667:1675	arg1	catalysis					1642:1650	general acid-base catalysis	1624:1650	general acid-base catalysis of crystalline cellulose	1624:1675	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	4	6	theme	accessible	801:810	arg1	cavity					823:828	a solvent accessible subsurface cavity	791:828	a solvent accessible subsurface cavity	791:828	Crystalline cellulose may be accommodated in a surface groove which is imperfectly bounded by the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces and thence digested in a solvent accessible subsurface cavity.					
31338614	4	7	theme	Crystalline	621:631	arg1	cellulose					633:641	Crystalline cellulose	621:641	Crystalline cellulose	621:641	Crystalline cellulose may be accommodated in a surface groove which is imperfectly bounded by the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces and thence digested in a solvent accessible subsurface cavity.					
31338614	6	8	theme	heterogeneous	1176:1188	arg1	composition					1201:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	2	9	theme	C	249:249	arg1	endoglucanases					255:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	2	9	theme	C	249:249	arg1	hydrolases					293:302	CBM49-encompassing hydrolases	274:302	CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose	274:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	10	10	dep	in	2059:2060	arg1	silico					2062:2067	silico	2062:2067	silico	2062:2067	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	10	11	theme	Graphical	1967:1975	arg1	digestion					2008:2016	Graphical Abstract Crystalline cellulose digestion	1967:2016	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.	1967:2091	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	9	12	theme	potential	1917:1925	arg1	carbohydrates					1939:1951	potential fermentable carbohydrates	1917:1951	potential fermentable carbohydrates from biomass	1917:1964	Results presented may aid the development of novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass.					
31338614	0	13	theme	GH9	86:88	arg1	endoglucanases					90:103	plant class C GH9 endoglucanases	72:103	plant class C GH9 endoglucanases	72:103	Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.					
31338614	2	14	theme	CBM49-encompassing	274:291	arg1	endoglucanases					255:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	2	14	theme	CBM49-encompassing	274:291	arg1	hydrolases					293:302	CBM49-encompassing hydrolases	274:302	CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose	274:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	8	15	theme	qualitative	1705:1715	arg1	differences					1717:1727	qualitative differences	1705:1727	qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members	1705:1801	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	6	16	theme	hydrophobic	1159:1169	arg1	composition					1201:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	6	17	theme	CBM49_linker	1245:1256	arg1	junction					1258:1265	the CBM49_linker junction	1241:1265	the CBM49_linker junction	1241:1265	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	6	18	from	role	1382:1385	arg1	catalysis					1400:1408	catalysis	1400:1408	catalysis	1400:1408	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	4	19	dep	thence	772:777	arg1	digested					779:786	digested	779:786	thence digested in a solvent accessible subsurface cavity	772:828	Crystalline cellulose may be accommodated in a surface groove which is imperfectly bounded by the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces and thence digested in a solvent accessible subsurface cavity.					
31338614	6	20	theme	conserved	1148:1156	arg1	composition					1201:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	10	21	theme	Crystalline	1986:1996	arg1	digestion					2008:2016	Graphical Abstract Crystalline cellulose digestion	1967:2016	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.	1967:2091	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	3	22	theme	CBM49	559:563	arg1	contribution					522:533	the contribution	518:533	the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion	518:618	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	10	23	theme	C	2033:2033	arg1	endoglucanases					2039:2052	plant class C GH9 endoglucanases	2021:2052	plant class C GH9 endoglucanases	2021:2052	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	6	24	theme	atomic	1224:1229	arg1	motion					1231:1236	increased atomic motion	1214:1236	increased atomic motion of the CBM49_linker junction	1214:1265	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	2	25	theme	Plant	237:241	arg1	endoglucanases					255:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	2	25	theme	Plant	237:241	arg1	hydrolases					293:302	CBM49-encompassing hydrolases	274:302	CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose	274:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	6	26	with	experiements	1280:1291	arg1	ligands					1298:1304	ligands	1298:1304	ligands of lower degrees of polymerization	1298:1339	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	10	27	theme	function	2083:2090	arg1	assessment					2069:2078	an in silico assessment	2056:2078	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.	1967:2091	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	10	28	theme	plant	2021:2025	arg1	endoglucanases					2039:2052	plant class C GH9 endoglucanases	2021:2052	plant class C GH9 endoglucanases	2021:2052	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	6	29	theme	degrees	1315:1321	arg1	ligands					1298:1304	ligands	1298:1304	ligands of lower degrees of polymerization	1298:1339	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	5	30	theme	polymer	1048:1054	arg1	geometry					1016:1023	a strained geometry	1005:1023	a strained geometry of the bound cellulose polymer	1005:1054	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	0	31	theme	crystalline	47:57	arg1	cellulose					59:67	crystalline cellulose	47:67	crystalline cellulose	47:67	Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.					
31338614	8	32	theme	class	1787:1791	arg1	members					1795:1801	class C members	1787:1801	class C members	1787:1801	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	8	33	theme	glycosidic	1754:1763	arg1	cleavage					1770:1777	glycosidic bond cleavage	1754:1777	glycosidic bond cleavage	1754:1777	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	7	34	theme	GH9	1498:1500	arg1	enzymes					1502:1508	CBM-containing plant and non-plant GH9 enzymes	1463:1508	CBM-containing plant and non-plant GH9 enzymes	1463:1508	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	7	34	theme	GH9	1498:1500	arg1	finding					1513:1519	a finding	1511:1519	a finding supported by exceptional sequence- and structural-homology	1511:1578	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	0	35	theme	class	78:82	arg1	endoglucanases					90:103	plant class C GH9 endoglucanases	72:103	plant class C GH9 endoglucanases	72:103	Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.					
31338614	5	36	theme	bound	1032:1036	arg1	polymer					1048:1054	the bound cellulose polymer	1028:1054	the bound cellulose polymer	1028:1054	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	5	37	theme	side	929:932	arg1	chains					934:939	the bulky side chains	919:939	the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer	919:1054	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	2	38	theme	D-glucopyranose	363:377	arg1	residues					379:386	contiguous D-glucopyranose residues	352:386	contiguous D-glucopyranose residues of crystalline cellulose	352:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	2	38	theme	D-glucopyranose	363:377	arg1	cellulose					403:411	crystalline cellulose	391:411	crystalline cellulose	391:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	6	39	theme	direct	1375:1380	arg1	role					1382:1385	a modulatory rather than direct role	1350:1385	a modulatory rather than direct role for CBM49 in catalysis	1350:1408	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	3	40	theme	characterised	452:464	arg1	enzymes					487:493	characterised and putative class C enzymes	452:493	characterised and putative class C enzymes	452:493	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	7	41	theme	plant	1478:1482	arg1	enzymes					1502:1508	CBM-containing plant and non-plant GH9 enzymes	1463:1508	CBM-containing plant and non-plant GH9 enzymes	1463:1508	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	7	41	theme	plant	1478:1482	arg1	finding					1513:1519	a finding	1511:1519	a finding supported by exceptional sequence- and structural-homology	1511:1578	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	0	42	theme	digestion	34:42	arg1	s					28:28	the mechanism(s)	14:29	the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases	14:103	Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.					
31338614	3	43	theme	digestion	610:618	arg1	s					582:582	the mechanism(s)	568:583	the mechanism(s) of crystalline cellulose digestion	568:618	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	2	44	theme	cellulose	403:411	arg1	residues					379:386	contiguous D-glucopyranose residues	352:386	contiguous D-glucopyranose residues of crystalline cellulose	352:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	2	44	theme	cellulose	403:411	arg1	cellulose					403:411	crystalline cellulose	391:411	crystalline cellulose	391:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	9	45	from	biomass	1958:1964	arg1	carbohydrates					1939:1951	potential fermentable carbohydrates	1917:1951	potential fermentable carbohydrates from biomass	1917:1964	Results presented may aid the development of novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass.					
31338614	3	46	theme	crystalline	588:598	arg1	digestion					610:618	crystalline cellulose digestion	588:618	crystalline cellulose digestion	588:618	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	3	47	theme	C	485:485	arg1	enzymes					487:493	characterised and putative class C enzymes	452:493	characterised and putative class C enzymes	452:493	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	6	48	theme	polymerization	1326:1339	arg1	degrees					1315:1321	lower degrees	1309:1321	lower degrees of polymerization	1309:1339	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	7	49	theme	facto	1443:1447	arg1	substrate					1449:1457	the de facto substrate	1436:1457	the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology	1436:1578	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	7	49	theme	facto	1443:1447	arg1	cellulose					1423:1431	Crystalline cellulose	1411:1431	Crystalline cellulose	1411:1431	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	4	50	theme	linker_CBM49	746:757	arg1	surfaces					759:766	the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces	715:766	the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces	715:766	Crystalline cellulose may be accommodated in a surface groove which is imperfectly bounded by the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces and thence digested in a solvent accessible subsurface cavity.					
31338614	3	51	theme	putative	470:477	arg1	enzymes					487:493	characterised and putative class C enzymes	452:493	characterised and putative class C enzymes	452:493	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	1	52	theme	fermentable	195:205	arg1	oils					231:234	potentially fermentable cellulose and vegetable oils	183:234	potentially fermentable cellulose and vegetable oils	183:234	Biofuels such as γ-valerolactone, bioethanol, and biodiesel are derived from potentially fermentable cellulose and vegetable oils.					
31338614	2	53	theme	crystalline	391:401	arg1	cellulose					403:411	crystalline cellulose	391:411	crystalline cellulose	391:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	5	54	theme	amino	953:957	arg1	acids					959:963	aromatic amino acids	944:963	aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer	944:1054	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	9	55	theme	novel	1849:1853	arg1	endoglucanases					1871:1884	novel plant-based GH9 endoglucanases	1849:1884	novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass	1849:1964	Results presented may aid the development of novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass.					
31338614	6	56	theme	lower	1309:1313	arg1	degrees					1315:1321	lower degrees	1309:1321	lower degrees of polymerization	1309:1339	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	6	57	theme	increased	1214:1222	arg1	motion					1231:1236	increased atomic motion	1214:1236	increased atomic motion of the CBM49_linker junction	1214:1265	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	2	58	theme	glycosidic	330:339	arg1	linkage					341:347	the β (1 → 4) glycosidic linkage	316:347	the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose	316:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	8	59	theme	general	1624:1630	arg1	catalysis					1642:1650	general acid-base catalysis	1624:1650	general acid-base catalysis of crystalline cellulose	1624:1675	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	9	60	theme	GH9	1867:1869	arg1	endoglucanases					1871:1884	novel plant-based GH9 endoglucanases	1849:1884	novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass	1849:1964	Results presented may aid the development of novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass.					
31338614	6	61	theme	amino	1190:1194	arg1	composition					1201:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	poorly conserved, hydrophobic, and heterogeneous amino acid composition	1141:1211	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	2	62	theme	GH9	251:253	arg1	endoglucanases					255:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	2	62	theme	GH9	251:253	arg1	hydrolases					293:302	CBM49-encompassing hydrolases	274:302	CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose	274:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	8	63	theme	crystalline	1655:1665	arg1	cellulose					1667:1675	crystalline cellulose	1655:1675	crystalline cellulose	1655:1675	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	6	64	theme	complete	1089:1096	arg1	absence					1098:1104	an almost complete absence	1079:1104	an almost complete absence of measurable cavities	1079:1127	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	3	65	theme	linker	547:552	arg1	contribution					522:533	the contribution	518:533	the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion	518:618	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	5	66	theme	physical	835:842	arg1	dimensions					844:853	The physical dimensions	831:853	The physical dimensions	831:853	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	4	67	theme	subsurface	812:821	arg1	cavity					823:828	a solvent accessible subsurface cavity	791:828	a solvent accessible subsurface cavity	791:828	Crystalline cellulose may be accommodated in a surface groove which is imperfectly bounded by the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces and thence digested in a solvent accessible subsurface cavity.					
31338614	8	68	from	differences	1717:1727	arg1	binding					1742:1748	substrate binding	1732:1748	substrate binding	1732:1748	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	8	68	from	differences	1717:1727	arg1	cleavage					1770:1777	glycosidic bond cleavage	1754:1777	glycosidic bond cleavage	1754:1777	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	3	69	theme	GH9	542:544	arg1	contribution					522:533	the contribution	518:533	the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion	518:618	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	9	70	theme	fermentable	1927:1937	arg1	carbohydrates					1939:1951	potential fermentable carbohydrates	1917:1951	potential fermentable carbohydrates from biomass	1917:1964	Results presented may aid the development of novel plant-based GH9 endoglucanases that could extract and utilise potential fermentable carbohydrates from biomass.					
31338614	3	71	theme	enzymes	487:493	arg1	models					442:447	3D-homology models	430:447	3D-homology models of characterised and putative class C enzymes	430:493	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	6	72	theme	junction	1258:1265	arg1	motion					1231:1236	increased atomic motion	1214:1236	increased atomic motion of the CBM49_linker junction	1214:1265	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	10	73	theme	cellulose	1998:2006	arg1	digestion					2008:2016	Graphical Abstract Crystalline cellulose digestion	1967:2016	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.	1967:2091	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	7	74	theme	exceptional	1534:1544	arg1	sequence-					1546:1554	exceptional sequence-	1534:1554	exceptional sequence-	1534:1554	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	4	75	theme	surface	668:674	arg1	groove					676:681	a surface groove	666:681	a surface groove which is imperfectly bounded by the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces	666:766	Crystalline cellulose may be accommodated in a surface groove which is imperfectly bounded by the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces and thence digested in a solvent accessible subsurface cavity.					
31338614	10	76	theme	Abstract	1977:1984	arg1	digestion					2008:2016	Graphical Abstract Crystalline cellulose digestion	1967:2016	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.	1967:2091	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	2	77	theme	class	243:247	arg1	endoglucanases					255:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases	237:268	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	2	77	theme	class	243:247	arg1	hydrolases					293:302	CBM49-encompassing hydrolases	274:302	CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose	274:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	8	78	theme	substrate	1732:1740	arg1	binding					1742:1748	substrate binding	1732:1748	substrate binding	1732:1748	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	5	79	theme	groove	887:892	arg1	distortions					859:869	distortions	859:869	distortions thereof	859:877	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	5	79	theme	groove	887:892	arg1	dimensions					844:853	The physical dimensions	831:853	The physical dimensions	831:853	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	6	80	dep	along	1130:1134	arg1	with					1136:1139	with	1136:1139	with	1136:1139	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	10	81	theme	class	2027:2031	arg1	endoglucanases					2039:2052	plant class C GH9 endoglucanases	2021:2052	plant class C GH9 endoglucanases	2021:2052	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	3	82	theme	mechanism	572:580	arg1	s					582:582	the mechanism(s)	568:583	the mechanism(s) of crystalline cellulose digestion	568:618	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	3	83	dep	GH9	542:544	arg1	the					538:540	the	538:540	the	538:540	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	6	84	theme	cavities	1120:1127	arg1	absence					1098:1104	an almost complete absence	1079:1104	an almost complete absence of measurable cavities	1079:1127	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	0	85	theme	cellulose	59:67	arg1	digestion					34:42	digestion	34:42	digestion of crystalline cellulose by plant class C GH9 endoglucanases	34:103	Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.					
31338614	8	86	theme	bond	1765:1768	arg1	cleavage					1770:1777	glycosidic bond cleavage	1754:1777	glycosidic bond cleavage	1754:1777	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	10	87	dep	digestion	2008:2016	arg1	assessment					2069:2078	an in silico assessment	2056:2078	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.	1967:2091	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	0	88	theme	plant	72:76	arg1	endoglucanases					90:103	plant class C GH9 endoglucanases	72:103	plant class C GH9 endoglucanases	72:103	Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.					
31338614	7	89	theme	non-plant	1488:1496	arg1	GH9					1498:1500	non-plant GH9	1488:1500	non-plant GH9	1488:1500	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	8	90	from	similarity	1610:1619	arg1	catalysis					1642:1650	general acid-base catalysis	1624:1650	general acid-base catalysis of crystalline cellulose	1624:1675	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	4	91	theme	GH9_CBM49	719:727	arg1	surfaces					759:766	the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces	715:766	the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces	715:766	Crystalline cellulose may be accommodated in a surface groove which is imperfectly bounded by the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces and thence digested in a solvent accessible subsurface cavity.					
31338614	10	92	theme	GH9	2035:2037	arg1	endoglucanases					2039:2052	plant class C GH9 endoglucanases	2021:2052	plant class C GH9 endoglucanases	2021:2052	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	0	93	theme	C	84:84	arg1	endoglucanases					90:103	plant class C GH9 endoglucanases	72:103	plant class C GH9 endoglucanases	72:103	Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.					
31338614	8	94	theme	C	1793:1793	arg1	members					1795:1801	class C members	1787:1801	class C members	1787:1801	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	5	95	theme	bulky	923:927	arg1	chains					934:939	the bulky side chains	919:939	the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer	919:1054	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	4	96	theme	GH9_linker	730:739	arg1	surfaces					759:766	the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces	715:766	the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces	715:766	Crystalline cellulose may be accommodated in a surface groove which is imperfectly bounded by the GH9_CBM49, GH9_linker, and linker_CBM49 surfaces and thence digested in a solvent accessible subsurface cavity.					
31338614	2	97	theme	residues	379:386	arg1	linkage					341:347	the β (1 → 4) glycosidic linkage	316:347	the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose	316:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	5	98	theme	cellulose	1038:1046	arg1	polymer					1048:1054	the bound cellulose polymer	1028:1054	the bound cellulose polymer	1028:1054	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	2	99	theme	contiguous	352:361	arg1	residues					379:386	contiguous D-glucopyranose residues	352:386	contiguous D-glucopyranose residues of crystalline cellulose	352:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	2	99	theme	contiguous	352:361	arg1	cellulose					403:411	crystalline cellulose	391:411	crystalline cellulose	391:411	Plant class C GH9 endoglucanases are CBM49-encompassing hydrolases that cleave the β (1 → 4) glycosidic linkage of contiguous D-glucopyranose residues of crystalline cellulose.					
31338614	0	100	theme	mechanism	18:26	arg1	s					28:28	the mechanism(s)	14:29	the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases	14:103	Insights into the mechanism(s) of digestion of crystalline cellulose by plant class C GH9 endoglucanases.					
31338614	3	101	theme	cellulose	600:608	arg1	digestion					610:618	crystalline cellulose digestion	588:618	crystalline cellulose digestion	588:618	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	6	102	theme	modulatory	1352:1361	arg1	role					1382:1385	a modulatory rather than direct role	1350:1385	a modulatory rather than direct role for CBM49 in catalysis	1350:1408	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	5	103	theme	acids	959:963	arg1	chains					934:939	the bulky side chains	919:939	the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer	919:1054	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	10	104	theme	in	2059:2060	arg1	assessment					2069:2078	an in silico assessment	2056:2078	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.	1967:2091	Graphical Abstract Crystalline cellulose digestion by plant class C GH9 endoglucanases - an in silico assessment of function.					
31338614	6	105	theme	docking	1272:1278	arg1	experiements					1280:1291	docking experiements	1272:1291	docking experiements with ligands of lower degrees of polymerization	1272:1339	These data along with an almost complete absence of measurable cavities, along with poorly conserved, hydrophobic, and heterogeneous amino acid composition, increased atomic motion of the CBM49_linker junction, and docking experiements with ligands of lower degrees of polymerization suggests a modulatory rather than direct role for CBM49 in catalysis.					
31338614	7	106	theme	CBM-containing	1463:1476	arg1	plant					1478:1482	CBM-containing plant	1463:1482	CBM-containing plant	1463:1482	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	1	107	attach	derived	170:176	arg2	bioethanol					140:149	bioethanol	140:149	bioethanol	140:149	Biofuels such as γ-valerolactone, bioethanol, and biodiesel are derived from potentially fermentable cellulose and vegetable oils.					
31338614	1	107	attach	derived	170:176	arg2	γ-valerolactone					123:137	γ-valerolactone	123:137	γ-valerolactone	123:137	Biofuels such as γ-valerolactone, bioethanol, and biodiesel are derived from potentially fermentable cellulose and vegetable oils.					
31338614	1	107	attach	derived	170:176	arg2	Biofuels					106:113	Biofuels	106:113	Biofuels such as γ-valerolactone, bioethanol, and biodiesel	106:164	Biofuels such as γ-valerolactone, bioethanol, and biodiesel are derived from potentially fermentable cellulose and vegetable oils.					
31338614	1	107	attach	derived	170:176	arg1	oils					231:234	potentially fermentable cellulose and vegetable oils	183:234	potentially fermentable cellulose and vegetable oils	183:234	Biofuels such as γ-valerolactone, bioethanol, and biodiesel are derived from potentially fermentable cellulose and vegetable oils.					
31338614	1	107	attach	derived	170:176	arg2	biodiesel					156:164	biodiesel	156:164	biodiesel	156:164	Biofuels such as γ-valerolactone, bioethanol, and biodiesel are derived from potentially fermentable cellulose and vegetable oils.					
31338614	5	108	theme	strained	1007:1014	arg1	geometry					1016:1023	a strained geometry	1005:1023	a strained geometry of the bound cellulose polymer	1005:1054	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	8	109	theme	implied	1602:1608	arg1	similarity					1610:1619	the implied similarity	1598:1619	the implied similarity in general acid-base catalysis of crystalline cellulose	1598:1675	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	3	110	theme	class	479:483	arg1	enzymes					487:493	characterised and putative class C enzymes	452:493	characterised and putative class C enzymes	452:493	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	1	111	theme	cellulose	207:215	arg1	oils					231:234	potentially fermentable cellulose and vegetable oils	183:234	potentially fermentable cellulose and vegetable oils	183:234	Biofuels such as γ-valerolactone, bioethanol, and biodiesel are derived from potentially fermentable cellulose and vegetable oils.					
31338614	7	112	theme	de	1440:1441	arg1	substrate					1449:1457	the de facto substrate	1436:1457	the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology	1436:1578	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	7	112	theme	de	1440:1441	arg1	cellulose					1423:1431	Crystalline cellulose	1411:1431	Crystalline cellulose	1411:1431	Crystalline cellulose is the de facto substrate for CBM-containing plant and non-plant GH9 enzymes, a finding supported by exceptional sequence- and structural-homology.					
31338614	5	113	theme	aromatic	944:951	arg1	acids					959:963	aromatic amino acids	944:963	aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer	944:1054	The physical dimensions and distortions thereof, of the groove, are mediated in part by the bulky side chains of aromatic amino acids that comprise it and may also result in a strained geometry of the bound cellulose polymer.					
31338614	3	114	theme	3D-homology	430:440	arg1	models					442:447	3D-homology models	430:447	3D-homology models of characterised and putative class C enzymes	430:493	Here, I analyse 3D-homology models of characterised and putative class C enzymes to glean insights into the contribution of the GH9, linker, and CBM49 to the mechanism(s) of crystalline cellulose digestion.					
31338614	8	115	theme	acid-base	1632:1640	arg1	catalysis					1642:1650	general acid-base catalysis	1624:1650	general acid-base catalysis of crystalline cellulose	1624:1675	However, despite the implied similarity in general acid-base catalysis of crystalline cellulose, this study also highlights qualitative differences in substrate binding and glycosidic bond cleavage amongst class C members.					
31338614	1	116	theme	vegetable	221:229	arg1	oils					231:234	potentially fermentable cellulose and vegetable oils	183:234	potentially fermentable cellulose and vegetable oils	183:234	Biofuels such as γ-valerolactone, bioethanol, and biodiesel are derived from potentially fermentable cellulose and vegetable oils.					
29325043	0	0	theme	Lipopolysaccharide	61:78	arg1	Synthesis					44:52	the Synthesis	40:52	the Synthesis of the Lipopolysaccharide	40:78	RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella.					
29325043	4	1	theme	outer	809:813	arg1	membrane					815:822	the outer membrane	805:822	the outer membrane	805:822	We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane.					
29325043	7	2	with	strain	1241:1246	arg1	phenotype					1273:1281	a hyperinflammatory phenotype	1253:1281	a hyperinflammatory phenotype	1253:1281	Its absence alters the normal synthesis of this macromolecule and affects the homeostasis of the outer membrane, resulting in a strain with a hyperinflammatory phenotype.					
29325043	3	3	theme	main	476:479	arg1	component					481:489	the main component	472:489	the main component of the outer membrane	472:511	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	3	3	theme	main	476:479	arg1	Lipopolysaccharide					446:463	Lipopolysaccharide	446:463	Lipopolysaccharide (LPS)	446:469	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	5	4	theme	LPS	908:910	arg1	composition					912:922	LPS composition	908:922	LPS composition	908:922	A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation.					
29325043	6	5	theme	biosynthetic	1074:1085	arg1	complex					1087:1093	the biosynthetic complex	1070:1093	the biosynthetic complex	1070:1093	We show that RomA is involved in the synthesis of LPS, probably coordinating part of the biosynthetic complex in the periplasm.					
29325043	3	6	theme	membrane	504:511	arg1	component					481:489	the main component	472:489	the main component of the outer membrane	472:511	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	3	6	theme	membrane	504:511	arg1	Lipopolysaccharide					446:463	Lipopolysaccharide	446:463	Lipopolysaccharide (LPS)	446:469	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	4	7	theme	membrane	815:822	arg1	homeostasis					790:800	the homeostasis	786:800	the homeostasis of the outer membrane	786:822	We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane.					
29325043	2	8	contain	have	409:412	arg1	components					393:402	their structural components	376:402	their structural components that have low proinflammatory activities	376:443	This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities.					
29325043	2	8	contain	have	409:412	arg2	activities					434:443	low proinflammatory activities	414:443	low proinflammatory activities	414:443	This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities.					
29325043	4	9	theme	periplasmic	703:713	arg1	protein					715:721	a novel periplasmic protein	695:721	a novel periplasmic protein (RomA)	695:728	We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane.					
29325043	4	9	theme	periplasmic	703:713	arg1	RomA					724:727	RomA	724:727	RomA	724:727	We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane.					
29325043	5	10	from	mutant	827:832	arg1	gene					842:845	this gene	837:845	this gene	837:845	A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation.					
29325043	6	11	theme	complex	1087:1093	arg1	part					1062:1065	part	1062:1065	part of the biosynthetic complex	1062:1093	We show that RomA is involved in the synthesis of LPS, probably coordinating part of the biosynthetic complex in the periplasm.					
29325043	1	12	theme	stealthy	158:165	arg1	Brucellaceae					141:152	Brucellaceae	141:152	Brucellaceae	141:152	Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response.					
29325043	1	12	theme	stealthy	158:165	arg1	pathogens					167:175	stealthy pathogens	158:175	stealthy pathogens	158:175	Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response.					
29325043	5	13	dep	showed	847:852	arg1	reduced					925:931	reduced	925:931	reduced adhesion	925:940	A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation.					
29325043	5	13	dep	showed	847:852	arg1	altered					900:906	altered	900:906	altered LPS composition	900:922	A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation.					
29325043	5	13	dep	showed	847:852	arg1	increased					947:955	increased	947:955	increased virulence and inflammation	947:982	A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation.					
29325043	3	14	theme	outer	498:502	arg1	membrane					504:511	the outer membrane	494:511	the outer membrane	494:511	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	2	15	theme	immune	355:360	arg1	system					362:367	the immune system	351:367	the immune system	351:367	This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities.					
29325043	2	16	theme	low	414:416	arg1	activities					434:443	low proinflammatory activities	414:443	low proinflammatory activities	414:443	This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities.					
29325043	3	17	theme	central	519:525	arg1	factor					537:542	a central virulence factor	517:542	a central virulence factor of Brucella	517:554	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	3	17	theme	central	519:525	arg1	Lipopolysaccharide					446:463	Lipopolysaccharide	446:463	Lipopolysaccharide (LPS)	446:469	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	3	18	theme	virulence	527:535	arg1	factor					537:542	a central virulence factor	517:542	a central virulence factor of Brucella	517:554	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	3	18	theme	virulence	527:535	arg1	Lipopolysaccharide					446:463	Lipopolysaccharide	446:463	Lipopolysaccharide (LPS)	446:469	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	0	19	theme	Periplasmic	8:18	arg1	RomA					0:3	RomA	0:3	RomA	0:3	RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella.					
29325043	0	19	theme	Periplasmic	8:18	arg1	Protein					20:26	A Periplasmic Protein	6:26	A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide	6:78	RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella.					
29325043	1	20	theme	strong	251:256	arg1	response					265:272	a strong immune response	249:272	a strong immune response	249:272	Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response.					
29325043	4	21	theme	novel	697:701	arg1	protein					715:721	a novel periplasmic protein	695:721	a novel periplasmic protein (RomA)	695:728	We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane.					
29325043	4	21	theme	novel	697:701	arg1	RomA					724:727	RomA	724:727	RomA	724:727	We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane.					
29325043	2	22	theme	structural	382:391	arg1	components					393:402	their structural components	376:402	their structural components that have low proinflammatory activities	376:443	This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities.					
29325043	4	23	dep	identification	656:669	arg1	the					652:654	the	652:654	the	652:654	We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane.					
29325043	1	24	theme	immune	258:263	arg1	response					265:272	a strong immune response	249:272	a strong immune response	249:272	Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response.					
29325043	0	25	theme	Inflammatory	96:107	arg1	Response					109:116	the Inflammatory Response	92:116	the Inflammatory Response Triggered by Brucella	92:138	RomA, A Periplasmic Protein Involved in the Synthesis of the Lipopolysaccharide, Tunes Down the Inflammatory Response Triggered by Brucella.					
29325043	5	26	theme	membrane	882:889	arg1	defects					891:897	membrane defects	882:897	membrane defects	882:897	A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation.					
29325043	1	27	theme	response	265:272	arg1	context					238:244	the context	234:244	the context of a strong immune response	234:272	Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response.					
29325043	3	28	theme	Brucella	547:554	arg1	factor					537:542	a central virulence factor	517:542	a central virulence factor of Brucella	517:554	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	3	28	theme	Brucella	547:554	arg1	Lipopolysaccharide					446:463	Lipopolysaccharide	446:463	Lipopolysaccharide (LPS)	446:469	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	7	29	theme	outer	1210:1214	arg1	membrane					1216:1223	the outer membrane	1206:1223	the outer membrane	1206:1223	Its absence alters the normal synthesis of this macromolecule and affects the homeostasis of the outer membrane, resulting in a strain with a hyperinflammatory phenotype.					
29325043	6	30	theme	LPS	1035:1037	arg1	synthesis					1022:1030	the synthesis	1018:1030	the synthesis of LPS	1018:1037	We show that RomA is involved in the synthesis of LPS, probably coordinating part of the biosynthetic complex in the periplasm.					
29325043	2	31	theme	proinflammatory	418:432	arg1	activities					434:443	low proinflammatory activities	414:443	low proinflammatory activities	414:443	This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities.					
29325043	3	32	theme	low	608:610	arg1	response					625:632	a low inflammatory response	606:632	a low inflammatory response	606:632	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	8	33	theme	proper	1313:1318	arg1	synthesis					1320:1328	the proper synthesis	1309:1328	the proper synthesis of LPS	1309:1335	Our results suggest that the proper synthesis of LPS is central to maximize virulence and minimize inflammation.					
29325043	8	33	theme	proper	1313:1318	arg1	central					1340:1346	central	1340:1346	central	1340:1346	Our results suggest that the proper synthesis of LPS is central to maximize virulence and minimize inflammation.					
29325043	2	34	theme	virulence	307:315	arg1	factors					317:323	several virulence factors	299:323	several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities	299:443	This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities.					
29325043	8	35	theme	LPS	1333:1335	arg1	synthesis					1320:1328	the proper synthesis	1309:1328	the proper synthesis of LPS	1309:1335	Our results suggest that the proper synthesis of LPS is central to maximize virulence and minimize inflammation.					
29325043	8	35	theme	LPS	1333:1335	arg1	central					1340:1346	central	1340:1346	central	1340:1346	Our results suggest that the proper synthesis of LPS is central to maximize virulence and minimize inflammation.					
29325043	3	36	theme	inflammatory	612:623	arg1	response					625:632	a low inflammatory response	606:632	a low inflammatory response	606:632	Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella, and it has been well established that it induces a low inflammatory response.					
29325043	7	37	theme	hyperinflammatory	1255:1271	arg1	phenotype					1273:1281	a hyperinflammatory phenotype	1253:1281	a hyperinflammatory phenotype	1253:1281	Its absence alters the normal synthesis of this macromolecule and affects the homeostasis of the outer membrane, resulting in a strain with a hyperinflammatory phenotype.					
29325043	2	38	theme	several	299:305	arg1	factors					317:323	several virulence factors	299:323	several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities	299:443	This capacity relies on several virulence factors that are able to modulate the immune system and in their structural components that have low proinflammatory activities.					
29325043	1	39	with	pathogens	167:175	arg1	ability					186:192	the ability to survive and replicate in the host in the context of a strong immune response	182:272	the ability to survive and replicate in the host in the context of a strong immune response	182:272	Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response.					
29325043	4	40	theme	protein	715:721	arg1	characterization					675:690	characterization	675:690	characterization	675:690	We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane.					
29325043	4	40	theme	protein	715:721	arg1	identification					656:669	identification	656:669	identification	656:669	We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, which is involved in the homeostasis of the outer membrane.					
29325043	5	41	theme	several	854:860	arg1	defects					891:897	membrane defects	882:897	membrane defects	882:897	A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation.					
29325043	5	41	theme	several	854:860	arg1	phenotypes					862:871	several phenotypes	854:871	several phenotypes	854:871	A mutant in this gene showed several phenotypes, such as membrane defects, altered LPS composition, reduced adhesion, and increased virulence and inflammation.					
29325043	7	42	theme	normal	1136:1141	arg1	synthesis					1143:1151	the normal synthesis	1132:1151	the normal synthesis of this macromolecule	1132:1173	Its absence alters the normal synthesis of this macromolecule and affects the homeostasis of the outer membrane, resulting in a strain with a hyperinflammatory phenotype.					
29325043	7	43	theme	membrane	1216:1223	arg1	homeostasis					1191:1201	the homeostasis	1187:1201	the homeostasis of the outer membrane	1187:1223	Its absence alters the normal synthesis of this macromolecule and affects the homeostasis of the outer membrane, resulting in a strain with a hyperinflammatory phenotype.					
29325043	7	44	theme	macromolecule	1161:1173	arg1	synthesis					1143:1151	the normal synthesis	1132:1151	the normal synthesis of this macromolecule	1132:1173	Its absence alters the normal synthesis of this macromolecule and affects the homeostasis of the outer membrane, resulting in a strain with a hyperinflammatory phenotype.					
31751722	3	0	theme	feed	579:582	arg1	ratio					595:599	the feed conversion ratio	575:599	the feed conversion ratio	575:599	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	4	1	theme	nutrient	732:739	arg1	digestibility					741:753	nutrient digestibility	732:753	nutrient digestibility	732:753	Dietary inulin supplementation improved eggshell thickness, nutrient digestibility and cecum Bacteroidales_S24-7_ group abundance in the laying hens.					
31751722	1	2	theme	nutrient	137:144	arg1	digestibility					146:158	nutrient digestibility	137:158	nutrient digestibility	137:158	Egg production performance, egg quality, nutrient digestibility, and microbial composition as affected by dietary inulin supplementation were evaluated in laying hens.					
31751722	5	3	theme	communities	1082:1092	arg1	digestibility					1019:1031	increased nutrient digestibility	1000:1031	increased nutrient digestibility	1000:1031	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	5	3	theme	communities	1082:1092	arg1	modulations					1047:1057	selective modulations	1037:1057	selective modulations of the cecum microbial communities	1037:1092	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	5	4	theme	increased	1000:1008	arg1	digestibility					1019:1031	increased nutrient digestibility	1000:1031	increased nutrient digestibility	1000:1031	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	2	5	theme	fed	322:324	arg1	diets					326:330	fed diets	322:330	fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively	322:410	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	1	6	theme	laying	251:256	arg1	hens					258:261	laying hens	251:261	laying hens	251:261	Egg production performance, egg quality, nutrient digestibility, and microbial composition as affected by dietary inulin supplementation were evaluated in laying hens.					
31751722	0	7	theme	hens	90:93	arg1	microbiota					69:78	the cecum microbiota	59:78	the cecum microbiota of laying hens	59:93	Inulin improves the egg production performance and affects the cecum microbiota of laying hens.					
31751722	5	8	theme	nutrient	1010:1017	arg1	digestibility					1019:1031	increased nutrient digestibility	1000:1031	increased nutrient digestibility	1000:1031	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	5	9	theme	dietary	837:843	arg1	supplementation					852:866	dietary inulin supplementation	837:866	dietary inulin supplementation	837:866	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	2	10	theme	laying	279:284	arg1	hens					286:289	300 laying hens	275:289	300 laying hens	275:289	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	0	11	theme	laying	83:88	arg1	hens					90:93	laying hens	83:93	laying hens	83:93	Inulin improves the egg production performance and affects the cecum microbiota of laying hens.					
31751722	5	12	theme	laying	973:978	arg1	hens					980:983	laying hens	973:983	laying hens	973:983	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	5	13	theme	inulin	845:850	arg1	supplementation					852:866	dietary inulin supplementation	837:866	dietary inulin supplementation	837:866	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	5	14	theme	hens	980:983	arg1	performance					935:945	egg production performance	920:945	egg production performance	920:945	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	5	14	theme	hens	980:983	arg1	thickness					960:968	eggshell thickness	951:968	eggshell thickness	951:968	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	1	15	dep	production	100:109	arg1	performance					111:121	performance	111:121	performance	111:121	Egg production performance, egg quality, nutrient digestibility, and microbial composition as affected by dietary inulin supplementation were evaluated in laying hens.					
31751722	2	16	theme	0	363:363	arg1	levels					353:358	levels	353:358	levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively	353:410	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	2	17	contain	containing	332:341	arg2	inulin					343:348	inulin	343:348	inulin	343:348	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	2	17	contain	containing	332:341	arg1	diets					326:330	fed diets	322:330	fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively	322:410	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	3	18	theme	supplementation	456:470	arg1	level					472:476	the 15 g/kg inulin supplementation level	437:476	the 15 g/kg inulin supplementation level	437:476	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	1	19	theme	microbial	165:173	arg1	composition					175:185	microbial composition	165:185	microbial composition	165:185	Egg production performance, egg quality, nutrient digestibility, and microbial composition as affected by dietary inulin supplementation were evaluated in laying hens.					
31751722	2	20	theme	10	379:380	arg1	levels					353:358	levels	353:358	levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively	353:410	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	3	21	theme	egg	495:497	arg1	weight					499:504	average egg weight	487:504	average egg weight by 2.54%	487:513	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	4	22	theme	eggshell	712:719	arg1	thickness					721:729	eggshell thickness	712:729	eggshell thickness	712:729	Dietary inulin supplementation improved eggshell thickness, nutrient digestibility and cecum Bacteroidales_S24-7_ group abundance in the laying hens.					
31751722	5	23	theme	eggshell	951:958	arg1	thickness					960:968	eggshell thickness	951:968	eggshell thickness	951:968	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	0	24	theme	egg	20:22	arg1	performance					35:45	the egg production performance	16:45	the egg production performance	16:45	Inulin improves the egg production performance and affects the cecum microbiota of laying hens.					
31751722	2	25	theme	hens	286:289	arg1	total					266:270	A total	264:270	A total of 300 laying hens	264:289	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	2	26	theme	5	376:376	arg1	levels					353:358	levels	353:358	levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively	353:410	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	5	27	theme	cecum	1066:1070	arg1	communities					1082:1092	the cecum microbial communities	1062:1092	the cecum microbial communities	1062:1092	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	3	28	theme	inulin	449:454	arg1	level					472:476	the 15 g/kg inulin supplementation level	437:476	the 15 g/kg inulin supplementation level	437:476	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	3	29	theme	laying	539:544	arg1	rate					546:549	laying rate	539:549	laying rate	539:549	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	1	30	theme	Egg	96:98	arg1	production					100:109	Egg production	96:109	Egg production	96:109	Egg production performance, egg quality, nutrient digestibility, and microbial composition as affected by dietary inulin supplementation were evaluated in laying hens.					
31751722	5	31	theme	microbial	1072:1080	arg1	communities					1082:1092	the cecum microbial communities	1062:1092	the cecum microbial communities	1062:1092	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	3	32	dep	weeks	658:662	arg1	8					669:669	8	669:669	8	669:669	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	4	33	theme	laying	809:814	arg1	hens					816:819	the laying hens	805:819	the laying hens	805:819	Dietary inulin supplementation improved eggshell thickness, nutrient digestibility and cecum Bacteroidales_S24-7_ group abundance in the laying hens.					
31751722	0	34	theme	production	24:33	arg1	performance					35:45	the egg production performance	16:45	the egg production performance	16:45	Inulin improves the egg production performance and affects the cecum microbiota of laying hens.					
31751722	2	35	theme	20 g/kg	390:396	arg1	levels					353:358	levels	353:358	levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively	353:410	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	3	36	theme	egg	516:518	arg1	mass					520:523	egg mass	516:523	egg mass by 5.76%	516:532	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	1	37	theme	dietary	202:208	arg1	supplementation					217:231	dietary inulin supplementation	202:231	dietary inulin supplementation	202:231	Egg production performance, egg quality, nutrient digestibility, and microbial composition as affected by dietary inulin supplementation were evaluated in laying hens.					
31751722	4	38	theme	Bacteroidales_S24-7_	765:784	arg1	abundance					792:800	cecum Bacteroidales_S24-7_ group abundance	759:800	cecum Bacteroidales_S24-7_ group abundance	759:800	Dietary inulin supplementation improved eggshell thickness, nutrient digestibility and cecum Bacteroidales_S24-7_ group abundance in the laying hens.					
31751722	4	39	theme	inulin	680:685	arg1	supplementation					687:701	Dietary inulin supplementation	672:701	Dietary inulin supplementation	672:701	Dietary inulin supplementation improved eggshell thickness, nutrient digestibility and cecum Bacteroidales_S24-7_ group abundance in the laying hens.					
31751722	3	40	theme	conversion	584:593	arg1	ratio					595:599	the feed conversion ratio	575:599	the feed conversion ratio	575:599	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	2	41	theme	15	383:384	arg1	levels					353:358	levels	353:358	levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively	353:410	A total of 300 laying hens were divided into 5 groups and fed diets containing inulin at levels of 0 (control), 5, 10, 15 and 20 g/kg, respectively.					
31751722	5	42	theme	selective	1037:1045	arg1	modulations					1047:1057	selective modulations	1037:1057	selective modulations of the cecum microbial communities	1037:1092	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	4	43	theme	cecum	759:763	arg1	abundance					792:800	cecum Bacteroidales_S24-7_ group abundance	759:800	cecum Bacteroidales_S24-7_ group abundance	759:800	Dietary inulin supplementation improved eggshell thickness, nutrient digestibility and cecum Bacteroidales_S24-7_ group abundance in the laying hens.					
31751722	3	44	dep	8	669:669	arg1	to					666:667	to	666:667	to	666:667	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	4	45	theme	Dietary	672:678	arg1	supplementation					687:701	Dietary inulin supplementation	672:701	Dietary inulin supplementation	672:701	Dietary inulin supplementation improved eggshell thickness, nutrient digestibility and cecum Bacteroidales_S24-7_ group abundance in the laying hens.					
31751722	5	46	dep	performance	935:945	arg1	the					916:918	the	916:918	the	916:918	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	5	47	theme	egg	920:922	arg1	performance					935:945	egg production performance	920:945	egg production performance	920:945	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	5	48	theme	15 g/kg	898:904	arg1	level					889:893	the level	885:893	the level of 15 g/kg	885:904	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	3	49	theme	average	487:493	arg1	weight					499:504	average egg weight	487:504	average egg weight by 2.54%	487:513	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	1	50	theme	inulin	210:215	arg1	supplementation					217:231	dietary inulin supplementation	202:231	dietary inulin supplementation	202:231	Egg production performance, egg quality, nutrient digestibility, and microbial composition as affected by dietary inulin supplementation were evaluated in laying hens.					
31751722	4	51	theme	group	786:790	arg1	abundance					792:800	cecum Bacteroidales_S24-7_ group abundance	759:800	cecum Bacteroidales_S24-7_ group abundance	759:800	Dietary inulin supplementation improved eggshell thickness, nutrient digestibility and cecum Bacteroidales_S24-7_ group abundance in the laying hens.					
31751722	5	52	theme	production	924:933	arg1	performance					935:945	egg production performance	920:945	egg production performance	920:945	In conclusion, dietary inulin supplementation, particularly at the level of 15 g/kg, improved the egg production performance and eggshell thickness of laying hens, mainly due to increased nutrient digestibility and selective modulations of the cecum microbial communities.					
31751722	1	53	theme	egg	124:126	arg1	quality					128:134	egg quality	124:134	egg quality	124:134	Egg production performance, egg quality, nutrient digestibility, and microbial composition as affected by dietary inulin supplementation were evaluated in laying hens.					
31751722	0	54	theme	cecum	63:67	arg1	microbiota					69:78	the cecum microbiota	59:78	the cecum microbiota of laying hens	59:93	Inulin improves the egg production performance and affects the cecum microbiota of laying hens.					
31751722	3	55	theme	feeding	650:656	arg1	weeks					658:662	feeding weeks 1 to 8	650:669	feeding weeks 1 to 8	650:669	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
31751722	3	56	theme	15 g/kg	441:447	arg1	level					472:476	the 15 g/kg inulin supplementation level	437:476	the 15 g/kg inulin supplementation level	437:476	The results showed that the 15 g/kg inulin supplementation level improved average egg weight by 2.54%, egg mass by 5.76%, and laying rate by 3.09%, and decreased the feed conversion ratio by 3.61% compared to those of the control during feeding weeks 1 to 8.					
30159959	0	0	theme	carcass	64:70	arg1	characteristics					72:86	carcass characteristics	64:86	carcass characteristics	64:86	Sweet potato vines in diets for growing rabbits on performance, carcass characteristics and meat quality.					
30159959	7	1	theme	Tukey	1075:1079	arg1	test					1081:1084	the Tukey test	1071:1084	the Tukey test (0.05)	1071:1091	The data obtained were statistically compared by analysis of variance followed by the Tukey test (0.05).					
30159959	7	1	theme	Tukey	1075:1079	arg1	0.05					1087:1090	0.05	1087:1090	0.05	1087:1090	The data obtained were statistically compared by analysis of variance followed by the Tukey test (0.05).					
30159959	1	2	from	ingredients	158:168	arg1	feed					180:183	animal feed	173:183	animal feed	173:183	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	4	3	theme	dietary	636:642	arg1	groups					644:649	three dietary groups	630:649	three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet	630:751	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	9	4	dep	hay	1229:1231	arg1	to					1236:1237	to	1236:1237	to	1236:1237	It can be concluded, therefore, that SPV may be used as a substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition.					
30159959	9	4	dep	hay	1229:1231	arg1	up					1233:1234	up	1233:1234	up	1233:1234	It can be concluded, therefore, that SPV may be used as a substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition.					
30159959	2	5	theme	potato	325:330	arg1	vines					332:336	sweet potato vines	319:336	sweet potato vines (SPV)	319:342	This study aimed to evaluate the viability of using sweet potato vines (SPV) as a replacement for alfalfa hay in diets for growing rabbits.					
30159959	2	5	theme	potato	325:330	arg1	replacement					349:359	a replacement	347:359	a replacement for alfalfa hay in diets for growing rabbits	347:404	This study aimed to evaluate the viability of using sweet potato vines (SPV) as a replacement for alfalfa hay in diets for growing rabbits.					
30159959	2	5	theme	potato	325:330	arg1	SPV					339:341	SPV	339:341	SPV	339:341	This study aimed to evaluate the viability of using sweet potato vines (SPV) as a replacement for alfalfa hay in diets for growing rabbits.					
30159959	4	6	theme	%	761:761	arg1	replacement					763:773	100% replacement	758:773	100% replacement of alfalfa hay by SPV	758:795	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	1	7	dep	use	110:112	arg1	substitutes					129:139	substitutes	129:139	substitutes for conventional ingredients in animal feed	129:183	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	2	8	from	hay	373:375	arg1	diets					380:384	diets	380:384	diets for growing rabbits	380:404	This study aimed to evaluate the viability of using sweet potato vines (SPV) as a replacement for alfalfa hay in diets for growing rabbits.					
30159959	9	9	theme	100	1239:1241	arg1	hay					1229:1231	hay	1229:1231	hay	1229:1231	It can be concluded, therefore, that SPV may be used as a substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition.					
30159959	0	10	from	rabbits	40:46	arg1	performance					51:61	performance	51:61	performance	51:61	Sweet potato vines in diets for growing rabbits on performance, carcass characteristics and meat quality.					
30159959	0	10	from	rabbits	40:46	arg1	characteristics					72:86	carcass characteristics	64:86	carcass characteristics	64:86	Sweet potato vines in diets for growing rabbits on performance, carcass characteristics and meat quality.					
30159959	0	10	from	rabbits	40:46	arg1	quality					97:103	meat quality	92:103	meat quality	92:103	Sweet potato vines in diets for growing rabbits on performance, carcass characteristics and meat quality.					
30159959	4	11	theme	100	758:760	arg1	%					761:761	%	761:761	%	761:761	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	1	12	theme	environmental	242:254	arg1	pollution					256:264	environmental pollution	242:264	environmental pollution	242:264	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	7	13	theme	variance	1050:1057	arg1	analysis					1038:1045	analysis	1038:1045	analysis of variance followed by the Tukey test (0.05)	1038:1091	The data obtained were statistically compared by analysis of variance followed by the Tukey test (0.05).					
30159959	9	14	dep	a	1204:1204	arg1	substitute					1206:1215	substitute	1206:1215	substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition	1206:1318	It can be concluded, therefore, that SPV may be used as a substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition.					
30159959	6	15	theme	glycogen	906:913	arg1	analysis					874:881	analysis	874:881	analysis of glucose and hepatic glycogen	874:913	The liver was collected for analysis of glucose and hepatic glycogen and meat samples were collected for analysis of the chemical composition.					
30159959	5	16	theme	age	841:843	arg1	77 days					830:836	77 days	830:836	77 days of age	830:843	The animals were slaughtered at 77 days of age.					
30159959	1	17	theme	conventional	145:156	arg1	ingredients					158:168	conventional ingredients	145:168	conventional ingredients in animal feed	145:183	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	0	18	theme	meat	92:95	arg1	quality					97:103	meat quality	92:103	meat quality	92:103	Sweet potato vines in diets for growing rabbits on performance, carcass characteristics and meat quality.					
30159959	6	19	theme	composition	976:986	arg1	analysis					951:958	analysis	951:958	analysis of the chemical composition	951:986	The liver was collected for analysis of glucose and hepatic glycogen and meat samples were collected for analysis of the chemical composition.					
30159959	0	20	theme	Sweet	0:4	arg1	potato					6:11	Sweet potato	0:11	Sweet potato	0:11	Sweet potato vines in diets for growing rabbits on performance, carcass characteristics and meat quality.					
30159959	3	21	theme	30 New	522:527	arg1	rabbits					537:543	30 New Zealand rabbits	522:543	30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g	522:596	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	2	22	theme	growing	390:396	arg1	rabbits					398:404	growing rabbits	390:404	growing rabbits	390:404	This study aimed to evaluate the viability of using sweet potato vines (SPV) as a replacement for alfalfa hay in diets for growing rabbits.					
30159959	4	23	with	diet	691:694	arg1	replacement					705:715	50% replacement	701:715	50% replacement of alfalfa hay by SPV	701:737	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	4	24	dep	diet	666:669	arg1	100SPV					740:745	100SPV	740:745	100SPV	740:745	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	4	24	dep	diet	666:669	arg1	50SPV					684:688	50SPV	684:688	50SPV	684:688	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	3	25	theme	Zealand	529:535	arg1	rabbits					537:543	30 New Zealand rabbits	522:543	30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g	522:596	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	1	26	theme	animal	173:178	arg1	feed					180:183	animal feed	173:183	animal feed	173:183	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	9	27	used	used	1196:1199	arg2	a					1204:1204	a	1204:1204	a	1204:1204	It can be concluded, therefore, that SPV may be used as a substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition.					
30159959	9	27	used	used	1196:1199	arg2	SPV					1185:1187	SPV	1185:1187	SPV	1185:1187	It can be concluded, therefore, that SPV may be used as a substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition.					
30159959	8	28	theme	analyzed	1106:1113	arg1	variables					1115:1123	the analyzed variables	1102:1123	the analyzed variables	1102:1123	None of the analyzed variables presented differences.					
30159959	4	29	theme	control	658:664	arg1	0SPV					652:655	0SPV	652:655	0SPV	652:655	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	4	29	theme	control	658:664	arg1	diet					666:669	control diet	658:669	control diet without SPV	658:681	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	4	29	theme	control	658:664	arg1	diet					748:751	diet	748:751	diet	748:751	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	4	29	theme	control	658:664	arg1	diet					691:694	diet	691:694	diet with 50% replacement of alfalfa hay by SPV	691:737	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	9	30	from	%	1242:1242	arg1	diets					1247:1251	diets	1247:1251	diets for rabbits	1247:1263	It can be concluded, therefore, that SPV may be used as a substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition.					
30159959	4	31	dep	groups	644:649	arg1	0SPV					652:655	0SPV	652:655	0SPV	652:655	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	4	31	dep	groups	644:649	arg1	diet					666:669	control diet	658:669	control diet without SPV	658:681	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	3	32	theme	metabolic	470:478	arg1	parameters					480:489	metabolic parameters	470:489	metabolic parameters	470:489	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	9	33	theme	meat	1303:1306	arg1	composition					1308:1318	meat composition	1303:1318	meat composition	1303:1318	It can be concluded, therefore, that SPV may be used as a substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition.					
30159959	3	34	theme	900 g	592:596	arg1	weight					582:587	an average weight	571:587	an average weight of 900 g	571:596	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	3	34	theme	900 g	592:596	arg1	days					558:561	35 days old	555:565	35 days old	555:565	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	4	35	theme	alfalfa	720:726	arg1	hay					728:730	alfalfa hay	720:730	alfalfa hay	720:730	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	6	36	theme	meat	919:922	arg1	samples					924:930	meat samples	919:930	meat samples	919:930	The liver was collected for analysis of glucose and hepatic glycogen and meat samples were collected for analysis of the chemical composition.					
30159959	6	37	theme	hepatic	898:904	arg1	glycogen					906:913	hepatic glycogen	898:913	hepatic glycogen	898:913	The liver was collected for analysis of glucose and hepatic glycogen and meat samples were collected for analysis of the chemical composition.					
30159959	3	38	theme	average	574:580	arg1	weight					582:587	an average weight	571:587	an average weight of 900 g	571:596	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	1	39	theme	promising	190:198	arg1	use					110:112	The use	106:112	The use of residues as substitutes for conventional ingredients in animal feed	106:183	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	1	39	theme	promising	190:198	arg1	alternative					200:210	a promising alternative	188:210	a promising alternative able to reduce both costs and environmental pollution	188:264	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	2	40	theme	sweet	319:323	arg1	vines					332:336	sweet potato vines	319:336	sweet potato vines (SPV)	319:342	This study aimed to evaluate the viability of using sweet potato vines (SPV) as a replacement for alfalfa hay in diets for growing rabbits.					
30159959	2	40	theme	sweet	319:323	arg1	replacement					349:359	a replacement	347:359	a replacement for alfalfa hay in diets for growing rabbits	347:404	This study aimed to evaluate the viability of using sweet potato vines (SPV) as a replacement for alfalfa hay in diets for growing rabbits.					
30159959	2	40	theme	sweet	319:323	arg1	SPV					339:341	SPV	339:341	SPV	339:341	This study aimed to evaluate the viability of using sweet potato vines (SPV) as a replacement for alfalfa hay in diets for growing rabbits.					
30159959	0	41	theme	growing	32:38	arg1	rabbits					40:46	growing rabbits	32:46	growing rabbits on performance, carcass characteristics and meat quality	32:103	Sweet potato vines in diets for growing rabbits on performance, carcass characteristics and meat quality.					
30159959	4	42	theme	hay	728:730	arg1	replacement					705:715	50% replacement	701:715	50% replacement of alfalfa hay by SPV	701:737	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	3	43	theme	meat	449:452	arg1	composition					454:464	meat composition	449:464	meat composition	449:464	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	4	44	theme	%	703:703	arg1	replacement					705:715	50% replacement	701:715	50% replacement of alfalfa hay by SPV	701:737	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	4	45	theme	hay	786:788	arg1	replacement					763:773	100% replacement	758:773	100% replacement of alfalfa hay by SPV	758:795	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	4	46	theme	50	701:702	arg1	%					703:703	%	703:703	%	703:703	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	3	47	theme	carcass	424:430	arg1	characteristics					432:446	carcass characteristics	424:446	carcass characteristics	424:446	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	6	48	theme	glucose	886:892	arg1	analysis					874:881	analysis	874:881	analysis of glucose and hepatic glycogen	874:913	The liver was collected for analysis of glucose and hepatic glycogen and meat samples were collected for analysis of the chemical composition.					
30159959	4	49	theme	alfalfa	778:784	arg1	hay					786:788	alfalfa hay	778:788	alfalfa hay	778:788	The animals were allotted into three dietary groups: 0SPV, control diet without SPV; 50SPV, diet with 50% replacement of alfalfa hay by SPV; 100SPV, diet with 100% replacement of alfalfa hay by SPV.					
30159959	3	50	theme	liver	498:502	arg1	performance					411:421	performance	411:421	performance	411:421	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	3	50	theme	liver	498:502	arg1	characteristics					432:446	carcass characteristics	424:446	carcass characteristics	424:446	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	3	50	theme	liver	498:502	arg1	composition					454:464	meat composition	449:464	meat composition	449:464	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	3	50	theme	liver	498:502	arg1	parameters					480:489	metabolic parameters	470:489	metabolic parameters	470:489	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	6	51	theme	chemical	967:974	arg1	composition					976:986	the chemical composition	963:986	the chemical composition	963:986	The liver was collected for analysis of glucose and hepatic glycogen and meat samples were collected for analysis of the chemical composition.					
30159959	1	52	theme	able	212:215	arg1	use					110:112	The use	106:112	The use of residues as substitutes for conventional ingredients in animal feed	106:183	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	1	52	theme	able	212:215	arg1	alternative					200:210	a promising alternative	188:210	a promising alternative able to reduce both costs and environmental pollution	188:264	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	8	53	theme	variables	1115:1123	arg1	None					1094:1097	None	1094:1097	None of the analyzed variables	1094:1123	None of the analyzed variables presented differences.					
30159959	3	54	dep	performance	411:421	arg1	The					407:409	The	407:409	The	407:409	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30159959	1	55	theme	residues	117:124	arg1	use					110:112	The use	106:112	The use of residues as substitutes for conventional ingredients in animal feed	106:183	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	1	55	theme	residues	117:124	arg1	alternative					200:210	a promising alternative	188:210	a promising alternative able to reduce both costs and environmental pollution	188:264	The use of residues as substitutes for conventional ingredients in animal feed is a promising alternative able to reduce both costs and environmental pollution.					
30159959	9	56	theme	alfalfa	1221:1227	arg1	hay					1229:1231	hay	1229:1231	hay	1229:1231	It can be concluded, therefore, that SPV may be used as a substitute for alfalfa hay up to 100% in diets for rabbits without impairing the performance and meat composition.					
30159959	3	57	theme	old	563:565	arg1	days					558:561	35 days old	555:565	35 days old	555:565	The performance, carcass characteristics, meat composition and metabolic parameters of the liver were evaluated in 30 New Zealand rabbits weaned at 35 days old and an average weight of 900 g.					
30301776	5	0	theme	incrementing	840:851	arg1	ploidy					853:858	incrementing ploidy	840:858	incrementing ploidy	840:858	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	7	1	theme	polysaccharide	1099:1112	arg1	content					1114:1120	matrix polysaccharide content	1092:1120	matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue	1092:1171	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	3	2	theme	rigorous	445:452	arg1	characterization					465:480	rigorous phenotypic characterization	445:480	rigorous phenotypic characterization	445:480	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	6	3	theme	inflorescence	874:886	arg1	highest					908:914	highest	908:914	highest	908:914	However, the inflorescence stem dry weight was highest in tetraploids.					
30301776	6	3	theme	inflorescence	874:886	arg1	weight					897:902	the inflorescence stem dry weight	870:902	the inflorescence stem dry weight	870:902	However, the inflorescence stem dry weight was highest in tetraploids.					
30301776	8	4	theme	higher	1187:1192	arg1	plants					1201:1206	higher ploidy plants	1187:1206	higher ploidy plants	1187:1206	In addition, higher ploidy plants displayed altered sugar composition.					
30301776	3	5	theme	phenotypic	454:463	arg1	characterization					465:480	rigorous phenotypic characterization	445:480	rigorous phenotypic characterization	445:480	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	5	6	theme	morphometric	681:692	arg1	analysis					694:701	morphometric analysis	681:701	morphometric analysis of leaves	681:711	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	7	7	theme	Cell	932:935	arg1	characterization					942:957	Cell wall characterization	932:957	Cell wall characterization	932:957	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	3	8	theme	Arabidopsis	344:354	arg1	plants					366:371	Arabidopsis (Arabidopsis thaliana) plants	331:371	Arabidopsis (Arabidopsis thaliana) plants	331:371	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	3	8	theme	Arabidopsis	344:354	arg1	thaliana					356:363	Arabidopsis thaliana	344:363	Arabidopsis thaliana	344:363	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	9	9	theme	delayed	1277:1283	arg1	development					1285:1295	the delayed development	1273:1295	the delayed development of polyploids	1273:1309	Such effects were linked to the delayed development of polyploids.					
30301776	7	10	theme	matrix	1092:1097	arg1	content					1114:1120	matrix polysaccharide content	1092:1120	matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue	1092:1171	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	9	11	attach	linked	1263:1268	arg2	effects					1250:1256	Such effects	1245:1256	Such effects	1245:1256	Such effects were linked to the delayed development of polyploids.					
30301776	9	11	attach	linked	1263:1268	arg1	development					1285:1295	the delayed development	1273:1295	the delayed development of polyploids	1273:1309	Such effects were linked to the delayed development of polyploids.					
30301776	7	12	theme	stem	1161:1164	arg1	tissue					1166:1171	the stem tissue	1157:1171	the stem tissue	1157:1171	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	11	13	theme	study	1422:1426	arg1	results					1406:1412	The results	1402:1412	The results of this study	1402:1426	The results of this study indicate that induction of polyploidy is a promising breeding strategy to further tailor crops for biomass production.					
30301776	10	14	theme	wall	1352:1355	arg1	composition					1357:1367	polyploid cell wall composition	1337:1367	polyploid cell wall composition	1337:1367	Moreover, the changes in polyploid cell wall composition promoted saccharification yield.					
30301776	3	15	theme	Arabidopsis	331:341	arg1	plants					366:371	Arabidopsis (Arabidopsis thaliana) plants	331:371	Arabidopsis (Arabidopsis thaliana) plants	331:371	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	3	15	theme	Arabidopsis	331:341	arg1	thaliana					356:363	Arabidopsis thaliana	344:363	Arabidopsis thaliana	344:363	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	4	16	dep	developed	525:533	arg1	compared					542:549	compared	542:549	developed slower compared to diploids	525:561	Kinematic analysis showed that polyploids developed slower compared to diploids; however, tetra- and hexaploids, but not octaploids, generated larger rosettes due to delayed flowering.					
30301776	5	17	theme	epidermal	745:753	arg1	cells					764:768	epidermal pavement cells	745:768	epidermal pavement cells	745:768	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	2	18	from	effect	217:222	arg1	composition					255:265	biomass composition	247:265	biomass composition	247:265	Although its potential to increase biomass yield is well described, the effect of polyploidization on biomass composition has largely remained unexplored.					
30301776	5	19	theme	leaf	824:827	arg1	blade					829:833	leaf blade	824:833	leaf blade with incrementing ploidy	824:858	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	3	20	theme	plants	366:371	arg1	series					321:326	a series	319:326	a series of Arabidopsis (Arabidopsis thaliana) plants	319:371	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	9	21	theme	polyploids	1300:1309	arg1	development					1285:1295	the delayed development	1273:1295	the delayed development of polyploids	1273:1309	Such effects were linked to the delayed development of polyploids.					
30301776	7	22	dep	hemicellulose	1128:1140	arg1	i.e.					1123:1126	i.e.	1123:1126	i.e.	1123:1126	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	8	23	theme	sugar	1226:1230	arg1	composition					1232:1242	altered sugar composition	1218:1242	altered sugar composition	1218:1242	In addition, higher ploidy plants displayed altered sugar composition.					
30301776	5	24	theme	increased	776:784	arg1	size					791:794	increased cell size	776:794	increased cell size	776:794	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	10	25	from	changes	1326:1332	arg1	composition					1357:1367	polyploid cell wall composition	1337:1367	polyploid cell wall composition	1337:1367	Moreover, the changes in polyploid cell wall composition promoted saccharification yield.					
30301776	0	26	theme	Cell	43:46	arg1	Composition					53:63	Cell Wall Composition	43:63	Cell Wall Composition	43:63	Polyploidy Affects Plant Growth and Alters Cell Wall Composition.					
30301776	4	27	theme	larger	626:631	arg1	rosettes					633:640	larger rosettes	626:640	larger rosettes due to delayed flowering	626:665	Kinematic analysis showed that polyploids developed slower compared to diploids; however, tetra- and hexaploids, but not octaploids, generated larger rosettes due to delayed flowering.					
30301776	5	28	theme	cell	786:789	arg1	size					791:794	increased cell size	776:794	increased cell size	776:794	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	7	29	theme	cellulose	1042:1050	arg1	content					1052:1058	cellulose content	1042:1058	cellulose content	1042:1058	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	1	30	theme	key	96:98	arg1	role					100:103	a key role	94:103	a key role	94:103	Polyploidization has played a key role in plant breeding and crop improvement.					
30301776	10	31	theme	saccharification	1378:1393	arg1	yield					1395:1399	saccharification yield	1378:1399	saccharification yield	1378:1399	Moreover, the changes in polyploid cell wall composition promoted saccharification yield.					
30301776	0	32	theme	Plant	19:23	arg1	Growth					25:30	Plant Growth	19:30	Plant Growth	19:30	Polyploidy Affects Plant Growth and Alters Cell Wall Composition.					
30301776	3	33	dep	generated	309:317	arg1	2n					411:412	2n	411:412	2n	411:412	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	4	34	theme	delayed	649:655	arg1	flowering					657:665	delayed flowering	649:665	delayed flowering	649:665	Kinematic analysis showed that polyploids developed slower compared to diploids; however, tetra- and hexaploids, but not octaploids, generated larger rosettes due to delayed flowering.					
30301776	5	35	theme	reduced	800:806	arg1	number					813:818	reduced cell number	800:818	reduced cell number per leaf blade with incrementing ploidy	800:858	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	3	36	theme	ploidy	396:401	arg1	levels					403:408	different somatic ploidy levels	378:408	different somatic ploidy levels	378:408	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	8	37	theme	altered	1218:1224	arg1	composition					1232:1242	altered sugar composition	1218:1242	altered sugar composition	1218:1242	In addition, higher ploidy plants displayed altered sugar composition.					
30301776	5	38	theme	cell	808:811	arg1	number					813:818	reduced cell number	800:818	reduced cell number per leaf blade with incrementing ploidy	800:858	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	5	39	theme	pavement	755:762	arg1	cells					764:768	epidermal pavement cells	745:768	epidermal pavement cells	745:768	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	11	40	theme	biomass	1527:1533	arg1	production					1535:1544	biomass production	1527:1544	biomass production	1527:1544	The results of this study indicate that induction of polyploidy is a promising breeding strategy to further tailor crops for biomass production.					
30301776	3	41	dep	2n	411:412	arg1	6n					419:420	6n	419:420	6n	419:420	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	3	41	dep	2n	411:412	arg1	8n					427:428	8n	427:428	8n	427:428	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	3	41	dep	2n	411:412	arg1	4n					415:416	4n	415:416	4n	415:416	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	7	42	theme	somatic	983:989	arg1	level					998:1002	the basic somatic ploidy level	973:1002	the basic somatic ploidy level	973:1002	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	5	43	theme	leaves	706:711	arg1	analysis					694:701	morphometric analysis	681:701	morphometric analysis of leaves	681:711	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	3	44	theme	different	378:386	arg1	levels					403:408	different somatic ploidy levels	378:408	different somatic ploidy levels	378:408	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	4	45	theme	Kinematic	483:491	arg1	analysis					493:500	Kinematic analysis	483:500	Kinematic analysis	483:500	Kinematic analysis showed that polyploids developed slower compared to diploids; however, tetra- and hexaploids, but not octaploids, generated larger rosettes due to delayed flowering.					
30301776	5	46	with	blade	829:833	arg1	ploidy					853:858	incrementing ploidy	840:858	incrementing ploidy	840:858	In addition, morphometric analysis of leaves showed that polyploidy affected epidermal pavement cells, with increased cell size and reduced cell number per leaf blade with incrementing ploidy.					
30301776	7	47	from	content	1114:1120	arg1	tissue					1166:1171	the stem tissue	1157:1171	the stem tissue	1157:1171	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	7	48	theme	ploidy	991:996	arg1	level					998:1002	the basic somatic ploidy level	973:1002	the basic somatic ploidy level	973:1002	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	3	49	theme	somatic	388:394	arg1	levels					403:408	different somatic ploidy levels	378:408	different somatic ploidy levels	378:408	Here, we generated a series of Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, and 8n) and performed rigorous phenotypic characterization.					
30301776	1	50	theme	plant	108:112	arg1	breeding					114:121	plant breeding	108:121	plant breeding	108:121	Polyploidization has played a key role in plant breeding and crop improvement.					
30301776	11	51	theme	promising	1471:1479	arg1	induction					1442:1450	induction	1442:1450	induction of polyploidy	1442:1464	The results of this study indicate that induction of polyploidy is a promising breeding strategy to further tailor crops for biomass production.					
30301776	11	51	theme	promising	1471:1479	arg1	strategy					1490:1497	a promising breeding strategy	1469:1497	a promising breeding strategy to further tailor crops for biomass production	1469:1544	The results of this study indicate that induction of polyploidy is a promising breeding strategy to further tailor crops for biomass production.					
30301776	6	52	theme	dry	893:895	arg1	highest					908:914	highest	908:914	highest	908:914	However, the inflorescence stem dry weight was highest in tetraploids.					
30301776	6	52	theme	dry	893:895	arg1	weight					897:902	the inflorescence stem dry weight	870:902	the inflorescence stem dry weight	870:902	However, the inflorescence stem dry weight was highest in tetraploids.					
30301776	2	53	theme	biomass	247:253	arg1	composition					255:265	biomass composition	247:265	biomass composition	247:265	Although its potential to increase biomass yield is well described, the effect of polyploidization on biomass composition has largely remained unexplored.					
30301776	7	54	theme	basic	977:981	arg1	level					998:1002	the basic somatic ploidy level	973:1002	the basic somatic ploidy level	973:1002	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	11	55	theme	breeding	1481:1488	arg1	induction					1442:1450	induction	1442:1450	induction of polyploidy	1442:1464	The results of this study indicate that induction of polyploidy is a promising breeding strategy to further tailor crops for biomass production.					
30301776	11	55	theme	breeding	1481:1488	arg1	strategy					1490:1497	a promising breeding strategy	1469:1497	a promising breeding strategy to further tailor crops for biomass production	1469:1544	The results of this study indicate that induction of polyploidy is a promising breeding strategy to further tailor crops for biomass production.					
30301776	9	56	theme	Such	1245:1248	arg1	effects					1250:1256	Such effects	1245:1256	Such effects	1245:1256	Such effects were linked to the delayed development of polyploids.					
30301776	10	57	theme	cell	1347:1350	arg1	composition					1357:1367	polyploid cell wall composition	1337:1367	polyploid cell wall composition	1337:1367	Moreover, the changes in polyploid cell wall composition promoted saccharification yield.					
30301776	11	58	theme	polyploidy	1455:1464	arg1	induction					1442:1450	induction	1442:1450	induction of polyploidy	1442:1464	The results of this study indicate that induction of polyploidy is a promising breeding strategy to further tailor crops for biomass production.					
30301776	11	58	theme	polyploidy	1455:1464	arg1	strategy					1490:1497	a promising breeding strategy	1469:1497	a promising breeding strategy to further tailor crops for biomass production	1469:1544	The results of this study indicate that induction of polyploidy is a promising breeding strategy to further tailor crops for biomass production.					
30301776	8	59	theme	ploidy	1194:1199	arg1	plants					1201:1206	higher ploidy plants	1187:1206	higher ploidy plants	1187:1206	In addition, higher ploidy plants displayed altered sugar composition.					
30301776	2	60	theme	biomass	180:186	arg1	yield					188:192	biomass yield	180:192	biomass yield	180:192	Although its potential to increase biomass yield is well described, the effect of polyploidization on biomass composition has largely remained unexplored.					
30301776	2	61	theme	polyploidization	227:242	arg1	effect					217:222	the effect	213:222	the effect of polyploidization on biomass composition	213:265	Although its potential to increase biomass yield is well described, the effect of polyploidization on biomass composition has largely remained unexplored.					
30301776	10	62	theme	polyploid	1337:1345	arg1	composition					1357:1367	polyploid cell wall composition	1337:1367	polyploid cell wall composition	1337:1367	Moreover, the changes in polyploid cell wall composition promoted saccharification yield.					
30301776	1	63	theme	crop	127:130	arg1	improvement					132:142	crop improvement	127:142	crop improvement	127:142	Polyploidization has played a key role in plant breeding and crop improvement.					
30301776	0	64	theme	Wall	48:51	arg1	Composition					53:63	Cell Wall Composition	43:63	Cell Wall Composition	43:63	Polyploidy Affects Plant Growth and Alters Cell Wall Composition.					
30301776	6	65	theme	stem	888:891	arg1	highest					908:914	highest	908:914	highest	908:914	However, the inflorescence stem dry weight was highest in tetraploids.					
30301776	6	65	theme	stem	888:891	arg1	weight					897:902	the inflorescence stem dry weight	870:902	the inflorescence stem dry weight	870:902	However, the inflorescence stem dry weight was highest in tetraploids.					
30301776	7	66	dep	content	1114:1120	arg1	hemicellulose					1128:1140	hemicellulose	1128:1140	hemicellulose	1128:1140	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	7	66	dep	content	1114:1120	arg1	pectin					1146:1151	pectin	1146:1151	pectin	1146:1151	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	7	67	theme	wall	937:940	arg1	characterization					942:957	Cell wall characterization	932:957	Cell wall characterization	932:957	Cell wall characterization revealed that the basic somatic ploidy level negatively correlated with lignin and cellulose content, and positively correlated with matrix polysaccharide content (i.e. hemicellulose and pectin) in the stem tissue.					
30301776	4	68	theme	due	642:644	arg1	rosettes					633:640	larger rosettes	626:640	larger rosettes due to delayed flowering	626:665	Kinematic analysis showed that polyploids developed slower compared to diploids; however, tetra- and hexaploids, but not octaploids, generated larger rosettes due to delayed flowering.					
29609177	1	0	theme	adsorption-UF	122:134	arg1	process					136:142	a pilot-scale adsorption-UF process	108:142	a pilot-scale adsorption-UF process equipped with an aerated system	108:174	In this study, a pilot-scale adsorption-UF process equipped with an aerated system is established for arsenic removal from As-spiked Songhua river water.					
29609177	1	1	theme	Songhua	226:232	arg1	water					240:244	As-spiked Songhua river water	216:244	As-spiked Songhua river water	216:244	In this study, a pilot-scale adsorption-UF process equipped with an aerated system is established for arsenic removal from As-spiked Songhua river water.					
29609177	2	2	attach	derived	337:343	arg2	PEI-coffee					316:325	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee)	247:326	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder	247:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	2	2	attach	derived	337:343	arg1	powder					363:368	spent coffee powder	350:368	spent coffee powder	350:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	10	3	theme	NaCl	1518:1521	arg1	agent					1502:1506	an eluting agent	1491:1506	an eluting agent of 10 wt% NaCl and 10 wt% NaOH	1491:1537	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	4	4	theme	negative	633:640	arg1	value					642:646	The negative value	629:646	The negative value of △H and △G	629:659	The negative value of △H and △G indicated the exothermic and spontaneous nature of As adsorption on PEI-coffee.					
29609177	1	5	theme	river	234:238	arg1	water					240:244	As-spiked Songhua river water	216:244	As-spiked Songhua river water	216:244	In this study, a pilot-scale adsorption-UF process equipped with an aerated system is established for arsenic removal from As-spiked Songhua river water.					
29609177	0	6	theme	membrane	75:82	arg1	process					84:90	UF membrane process	72:90	Arsenic removal from water and river water by the combined adsorption - UF membrane process.	0:91	Arsenic removal from water and river water by the combined adsorption - UF membrane process.					
29609177	2	7	from	water	427:431	arg1	removal					414:420	arsenic removal	406:420	arsenic removal from water	406:431	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	2	8	theme	amino-functionalized	267:286	arg1	PEI-coffee					316:325	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee)	247:326	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder	247:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	3	9	theme	maximum	542:548	arg1	capacity					561:568	a maximum adsorption capacity	540:568	a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively	540:626	The batch experiments revealed that the adsorption process could be well described by Langmuir model with a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively.					
29609177	0	10	theme	UF	72:73	arg1	process					84:90	UF membrane process	72:90	Arsenic removal from water and river water by the combined adsorption - UF membrane process.	0:91	Arsenic removal from water and river water by the combined adsorption - UF membrane process.					
29609177	4	11	theme	spontaneous	690:700	arg1	nature					702:707	the exothermic and spontaneous nature	671:707	the exothermic and spontaneous nature of As adsorption on PEI-coffee	671:738	The negative value of △H and △G indicated the exothermic and spontaneous nature of As adsorption on PEI-coffee.					
29609177	4	12	from	nature	702:707	arg1	PEI-coffee					729:738	PEI-coffee	729:738	PEI-coffee	729:738	The negative value of △H and △G indicated the exothermic and spontaneous nature of As adsorption on PEI-coffee.					
29609177	2	13	theme	synthesized	255:265	arg1	PEI-coffee					316:325	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee)	247:326	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder	247:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	5	14	theme	surface	860:866	arg1	RSM					881:883	RSM	881:883	RSM	881:883	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	5	14	theme	surface	860:866	arg1	methodology					868:878	response surface methodology	851:878	response surface methodology (RSM) based on a central composite design (CCD)	851:926	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	7	15	theme	removal	1092:1098	arg1	efficiency					1100:1109	the removal efficiency	1088:1109	the removal efficiency by oxidizing As(III) to As(V)	1088:1139	It was demonstrated that aeration not only increased the removal efficiency by oxidizing As(III) to As(V), but mitigated the membrane fouling process.					
29609177	0	16	from	water	37:41	arg1	removal					8:14	Arsenic removal	0:14	Arsenic removal from water and river water by the combined adsorption - UF membrane process.	0:91	Arsenic removal from water and river water by the combined adsorption - UF membrane process.					
29609177	6	17	theme	UF	955:956	arg1	process					958:964	UF process	955:964	UF process	955:964	The combined adsorption - UF process was employed for arsenic removal from As-spiked Songhua river water.					
29609177	4	18	theme	As	712:713	arg1	adsorption					715:724	As adsorption	712:724	As adsorption	712:724	The negative value of △H and △G indicated the exothermic and spontaneous nature of As adsorption on PEI-coffee.					
29609177	10	19	theme	eluting	1494:1500	arg1	agent					1502:1506	an eluting agent	1491:1506	an eluting agent of 10 wt% NaCl and 10 wt% NaOH	1491:1537	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	6	20	theme	arsenic	983:989	arg1	removal					991:997	arsenic removal	983:997	arsenic removal from As-spiked Songhua river water	983:1032	The combined adsorption - UF process was employed for arsenic removal from As-spiked Songhua river water.					
29609177	10	21	theme	%	1532:1532	arg1	NaOH					1534:1537	10 wt% NaOH	1527:1537	10 wt% NaOH	1527:1537	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	9	22	theme	suspended	1380:1388	arg1	solids					1390:1395	suspended solids	1380:1395	suspended solids (SS)	1380:1400	After UF filtration, the dissolved As, suspended solids (SS), and TOC can be effectively eliminated.					
29609177	9	22	theme	suspended	1380:1388	arg1	SS					1398:1399	SS	1398:1399	SS	1398:1399	After UF filtration, the dissolved As, suspended solids (SS), and TOC can be effectively eliminated.					
29609177	3	23	theme	Langmuir	520:527	arg1	model					529:533	Langmuir model	520:533	Langmuir model with a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively	520:626	The batch experiments revealed that the adsorption process could be well described by Langmuir model with a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively.					
29609177	5	24	theme	initial	789:795	arg1	pH					785:786	pH	785:786	pH	785:786	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	5	24	theme	initial	789:795	arg1	concentration					797:809	initial concentration	789:809	initial concentration	789:809	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	10	25	theme	high	1589:1592	arg1	capacity					1605:1612	a very high adsorption capacity	1582:1612	a very high adsorption capacity	1582:1612	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	3	26	theme	adsorption	550:559	arg1	capacity					561:568	a maximum adsorption capacity	540:568	a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively	540:626	The batch experiments revealed that the adsorption process could be well described by Langmuir model with a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively.					
29609177	9	27	theme	UF	1347:1348	arg1	filtration					1350:1359	UF filtration	1347:1359	UF filtration	1347:1359	After UF filtration, the dissolved As, suspended solids (SS), and TOC can be effectively eliminated.					
29609177	0	28	theme	Arsenic	0:6	arg1	removal					8:14	Arsenic removal	0:14	Arsenic removal from water and river water by the combined adsorption - UF membrane process.	0:91	Arsenic removal from water and river water by the combined adsorption - UF membrane process.					
29609177	10	29	theme	10 wt	1527:1531	arg1	NaOH					1534:1537	10 wt% NaOH	1527:1537	10 wt% NaOH	1527:1537	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	2	30	theme	adsorbent	305:313	arg1	PEI-coffee					316:325	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee)	247:326	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder	247:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	1	31	theme	aerated	161:167	arg1	system					169:174	an aerated system	158:174	an aerated system	158:174	In this study, a pilot-scale adsorption-UF process equipped with an aerated system is established for arsenic removal from As-spiked Songhua river water.					
29609177	1	32	from	water	240:244	arg1	removal					203:209	arsenic removal	195:209	arsenic removal from As-spiked Songhua river water	195:244	In this study, a pilot-scale adsorption-UF process equipped with an aerated system is established for arsenic removal from As-spiked Songhua river water.					
29609177	2	33	theme	cellulose	295:303	arg1	PEI-coffee					316:325	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee)	247:326	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder	247:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	2	34	theme	arsenic	406:412	arg1	removal					414:420	arsenic removal	406:420	arsenic removal from water	406:431	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	5	35	theme	operating	756:764	arg1	parameters					766:775	operating parameters	756:775	operating parameters such as pH, initial concentration and adsorbent dosage,	756:831	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	5	35	theme	operating	756:764	arg1	pH					785:786	pH	785:786	pH	785:786	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	8	36	theme	UF	1221:1222	arg1	membrane					1224:1231	UF membrane	1221:1231	UF membrane	1221:1231	Besides of the adsorption process, UF membrane could also reject arsenic through the electrostatic repulsion between arsenic species and membrane surface.					
29609177	8	37	theme	membrane	1323:1330	arg1	surface					1332:1338	membrane surface	1323:1338	membrane surface	1323:1338	Besides of the adsorption process, UF membrane could also reject arsenic through the electrostatic repulsion between arsenic species and membrane surface.					
29609177	2	38	theme	coffee	288:293	arg1	PEI-coffee					316:325	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee)	247:326	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder	247:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	5	39	theme	adsorbent	815:823	arg1	pH					785:786	pH	785:786	pH	785:786	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	5	39	theme	adsorbent	815:823	arg1	dosage					825:830	adsorbent dosage	815:830	adsorbent dosage	815:830	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	0	40	theme	river	31:35	arg1	water					37:41	river water	31:41	river water	31:41	Arsenic removal from water and river water by the combined adsorption - UF membrane process.					
29609177	7	41	theme	membrane	1160:1167	arg1	process					1177:1183	the membrane fouling process	1156:1183	the membrane fouling process	1156:1183	It was demonstrated that aeration not only increased the removal efficiency by oxidizing As(III) to As(V), but mitigated the membrane fouling process.					
29609177	10	42	theme	process	1656:1662	arg1	cycles					1622:1627	6 cycles	1620:1627	6 cycles of adsorption-regeneration process	1620:1662	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	10	43	theme	%	1516:1516	arg1	NaCl					1518:1521	10 wt% NaCl	1511:1521	10 wt% NaCl	1511:1521	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	6	44	theme	combined	933:940	arg1	adsorption					942:951	The combined adsorption	929:951	The combined adsorption	929:951	The combined adsorption - UF process was employed for arsenic removal from As-spiked Songhua river water.					
29609177	3	45	theme	46.1 mg/g	582:590	arg1	capacity					561:568	a maximum adsorption capacity	540:568	a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively	540:626	The batch experiments revealed that the adsorption process could be well described by Langmuir model with a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively.					
29609177	6	46	from	water	1028:1032	arg1	removal					991:997	arsenic removal	983:997	arsenic removal from As-spiked Songhua river water	983:1032	The combined adsorption - UF process was employed for arsenic removal from As-spiked Songhua river water.					
29609177	3	47	theme	batch	438:442	arg1	experiments					444:454	The batch experiments	434:454	The batch experiments	434:454	The batch experiments revealed that the adsorption process could be well described by Langmuir model with a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively.					
29609177	3	48	with	model	529:533	arg1	capacity					561:568	a maximum adsorption capacity	540:568	a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively	540:626	The batch experiments revealed that the adsorption process could be well described by Langmuir model with a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively.					
29609177	8	49	theme	electrostatic	1271:1283	arg1	repulsion					1285:1293	the electrostatic repulsion	1267:1293	the electrostatic repulsion between arsenic species and membrane surface	1267:1338	Besides of the adsorption process, UF membrane could also reject arsenic through the electrostatic repulsion between arsenic species and membrane surface.					
29609177	2	50	used	used	397:400	arg2	PEI-coffee					316:325	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee)	247:326	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder	247:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	3	51	theme	adsorption	474:483	arg1	process					485:491	the adsorption process	470:491	the adsorption process	470:491	The batch experiments revealed that the adsorption process could be well described by Langmuir model with a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively.					
29609177	10	52	theme	10 wt	1511:1515	arg1	NaCl					1518:1521	10 wt% NaCl	1511:1521	10 wt% NaCl	1511:1521	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	3	53	theme	13.2	573:576	arg1	capacity					561:568	a maximum adsorption capacity	540:568	a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively	540:626	The batch experiments revealed that the adsorption process could be well described by Langmuir model with a maximum adsorption capacity of 13.2 and 46.1 mg/g for As(III) and As(V), respectively.					
29609177	10	54	theme	NaOH	1534:1537	arg1	agent					1502:1506	an eluting agent	1491:1506	an eluting agent of 10 wt% NaCl and 10 wt% NaOH	1491:1537	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	10	55	theme	adsorption	1594:1603	arg1	capacity					1605:1612	a very high adsorption capacity	1582:1612	a very high adsorption capacity	1582:1612	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	4	56	theme	△H	651:652	arg1	value					642:646	The negative value	629:646	The negative value of △H and △G	629:659	The negative value of △H and △G indicated the exothermic and spontaneous nature of As adsorption on PEI-coffee.					
29609177	5	57	theme	composite	905:913	arg1	CCD					923:925	CCD	923:925	CCD	923:925	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	5	57	theme	composite	905:913	arg1	design					915:920	a central composite design	895:920	a central composite design (CCD)	895:926	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	10	58	theme	adsorption-regeneration	1632:1654	arg1	process					1656:1662	adsorption-regeneration process	1632:1662	adsorption-regeneration process	1632:1662	The saturated adsorbent was regenerated by using an eluting agent of 10 wt% NaCl and 10 wt% NaOH, the regenerated adsorbent still sustained a very high adsorption capacity after 6 cycles of adsorption-regeneration process.					
29609177	4	59	theme	△G	658:659	arg1	value					642:646	The negative value	629:646	The negative value of △H and △G	629:659	The negative value of △H and △G indicated the exothermic and spontaneous nature of As adsorption on PEI-coffee.					
29609177	6	60	theme	river	1022:1026	arg1	water					1028:1032	As-spiked Songhua river water	1004:1032	As-spiked Songhua river water	1004:1032	The combined adsorption - UF process was employed for arsenic removal from As-spiked Songhua river water.					
29609177	1	61	theme	arsenic	195:201	arg1	removal					203:209	arsenic removal	195:209	arsenic removal from As-spiked Songhua river water	195:244	In this study, a pilot-scale adsorption-UF process equipped with an aerated system is established for arsenic removal from As-spiked Songhua river water.					
29609177	5	62	theme	central	897:903	arg1	CCD					923:925	CCD	923:925	CCD	923:925	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	5	62	theme	central	897:903	arg1	design					915:920	a central composite design	895:920	a central composite design (CCD)	895:926	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	4	63	theme	adsorption	715:724	arg1	nature					702:707	the exothermic and spontaneous nature	671:707	the exothermic and spontaneous nature of As adsorption on PEI-coffee	671:738	The negative value of △H and △G indicated the exothermic and spontaneous nature of As adsorption on PEI-coffee.					
29609177	4	64	theme	exothermic	675:684	arg1	nature					702:707	the exothermic and spontaneous nature	671:707	the exothermic and spontaneous nature of As adsorption on PEI-coffee	671:738	The negative value of △H and △G indicated the exothermic and spontaneous nature of As adsorption on PEI-coffee.					
29609177	2	65	theme	spent	350:354	arg1	powder					363:368	spent coffee powder	350:368	spent coffee powder	350:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	8	66	theme	adsorption	1201:1210	arg1	process					1212:1218	the adsorption process	1197:1218	the adsorption process	1197:1218	Besides of the adsorption process, UF membrane could also reject arsenic through the electrostatic repulsion between arsenic species and membrane surface.					
29609177	5	67	theme	response	851:858	arg1	RSM					881:883	RSM	881:883	RSM	881:883	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	5	67	theme	response	851:858	arg1	methodology					868:878	response surface methodology	851:878	response surface methodology (RSM) based on a central composite design (CCD)	851:926	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	0	68	theme	combined	50:57	arg1	adsorption					59:68	the combined adsorption	46:68	the combined adsorption	46:68	Arsenic removal from water and river water by the combined adsorption - UF membrane process.					
29609177	6	69	theme	As-spiked	1004:1012	arg1	water					1028:1032	As-spiked Songhua river water	1004:1032	As-spiked Songhua river water	1004:1032	The combined adsorption - UF process was employed for arsenic removal from As-spiked Songhua river water.					
29609177	0	70	dep	removal	8:14	arg1	process					84:90	UF membrane process	72:90	Arsenic removal from water and river water by the combined adsorption - UF membrane process.	0:91	Arsenic removal from water and river water by the combined adsorption - UF membrane process.					
29609177	5	71	theme	parameters	766:775	arg1	effects					745:751	The effects	741:751	The effects of operating parameters such as pH, initial concentration and adsorbent dosage,	741:831	The effects of operating parameters such as pH, initial concentration and adsorbent dosage, were optimized by response surface methodology (RSM) based on a central composite design (CCD).					
29609177	2	72	theme	coffee	356:361	arg1	powder					363:368	spent coffee powder	350:368	spent coffee powder	350:368	A newly synthesized amino-functionalized coffee cellulose adsorbent (PEI-coffee) which is derived from spent coffee powder is fully characterized and used for arsenic removal from water.					
29609177	7	73	theme	fouling	1169:1175	arg1	process					1177:1183	the membrane fouling process	1156:1183	the membrane fouling process	1156:1183	It was demonstrated that aeration not only increased the removal efficiency by oxidizing As(III) to As(V), but mitigated the membrane fouling process.					
29609177	6	74	theme	Songhua	1014:1020	arg1	water					1028:1032	As-spiked Songhua river water	1004:1032	As-spiked Songhua river water	1004:1032	The combined adsorption - UF process was employed for arsenic removal from As-spiked Songhua river water.					
29609177	0	75	from	water	21:25	arg1	removal					8:14	Arsenic removal	0:14	Arsenic removal from water and river water by the combined adsorption - UF membrane process.	0:91	Arsenic removal from water and river water by the combined adsorption - UF membrane process.					
29609177	8	76	theme	arsenic	1303:1309	arg1	species					1311:1317	arsenic species	1303:1317	arsenic species	1303:1317	Besides of the adsorption process, UF membrane could also reject arsenic through the electrostatic repulsion between arsenic species and membrane surface.					
29609177	1	77	theme	pilot-scale	110:120	arg1	process					136:142	a pilot-scale adsorption-UF process	108:142	a pilot-scale adsorption-UF process equipped with an aerated system	108:174	In this study, a pilot-scale adsorption-UF process equipped with an aerated system is established for arsenic removal from As-spiked Songhua river water.					
29609177	1	78	theme	As-spiked	216:224	arg1	water					240:244	As-spiked Songhua river water	216:244	As-spiked Songhua river water	216:244	In this study, a pilot-scale adsorption-UF process equipped with an aerated system is established for arsenic removal from As-spiked Songhua river water.					
29438883	12	0	theme	cartilage	1836:1844	arg1	regeneration					1846:1857	hyaline cartilage regeneration	1828:1857	hyaline cartilage regeneration	1828:1857	Thus, the developed nano-engineered gradient hydrogel enhanced hyaline cartilage regeneration with subchondral bone formation and lateral host-tissue integration.					
29438883	7	1	theme	rabbit	1135:1140	arg1	defect					1156:1161	rabbit osteochondral defect	1135:1161	rabbit osteochondral defect	1135:1161	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels was compared with control using rabbit osteochondral defect after 3 and 8 weeks.					
29438883	2	2	theme	subchondral	415:425	arg1	zones					436:440	chondral and subchondral hydrogel zones	402:440	chondral and subchondral hydrogel zones respectively	402:453	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	12	3	theme	bone	1876:1879	arg1	formation					1881:1889	subchondral bone formation	1864:1889	subchondral bone formation	1864:1889	Thus, the developed nano-engineered gradient hydrogel enhanced hyaline cartilage regeneration with subchondral bone formation and lateral host-tissue integration.					
29438883	4	4	theme	ChS-NPs	713:719	arg1	interface					692:700	gradient interface	683:700	gradient interface of nHA and ChS-NPs	683:719	Osteochondral hydrogel exhibited interconnected porous structure and spatial variation with gradient interface of nHA and ChS-NPs.					
29438883	8	5	theme	other	1271:1275	arg1	groups					1277:1282	other groups	1271:1282	other groups	1271:1282	Complete closure of defect was observed in gradient (8 weeks) while defect remained in other groups.					
29438883	8	6	theme	Complete	1184:1191	arg1	closure					1193:1199	Complete closure	1184:1199	Complete closure of defect	1184:1209	Complete closure of defect was observed in gradient (8 weeks) while defect remained in other groups.					
29438883	4	7	theme	porous	639:644	arg1	structure					646:654	interconnected porous structure	624:654	interconnected porous structure	624:654	Osteochondral hydrogel exhibited interconnected porous structure and spatial variation with gradient interface of nHA and ChS-NPs.					
29438883	5	8	theme	predominant	802:812	arg1	∼930 Pa					831:837	∼930 Pa	831:837	∼930 Pa	831:837	Microcomputed tomography (μCT) demonstrated nHA gradation while rheology showed predominant elastic modulus (∼930 Pa) at the interface.					
29438883	5	8	theme	predominant	802:812	arg1	modulus					822:828	predominant elastic modulus	802:828	predominant elastic modulus (∼930 Pa)	802:838	Microcomputed tomography (μCT) demonstrated nHA gradation while rheology showed predominant elastic modulus (∼930 Pa) at the interface.					
29438883	6	9	theme	cell-cell	968:976	arg1	interaction					978:988	cell-cell interaction	968:988	cell-cell interaction at the interface	968:1005	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	9	10	theme	collagen	1308:1315	arg1	deposition					1339:1348	collagen and glycosaminoglycan deposition	1308:1348	deposition	1339:1348	Histology demonstrated collagen and glycosaminoglycan deposition in neo-matrix and presence of hyaline cartilage-characteristic matrix, chondrocytes and osteoblasts.					
29438883	10	11	theme	mineral	1550:1556	arg1	density					1558:1564	higher bone mineral density	1538:1564	higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group	1538:1637	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	5	12	theme	elastic	814:820	arg1	∼930 Pa					831:837	∼930 Pa	831:837	∼930 Pa	831:837	Microcomputed tomography (μCT) demonstrated nHA gradation while rheology showed predominant elastic modulus (∼930 Pa) at the interface.					
29438883	5	12	theme	elastic	814:820	arg1	modulus					822:828	predominant elastic modulus	802:828	predominant elastic modulus (∼930 Pa)	802:838	Microcomputed tomography (μCT) demonstrated nHA gradation while rheology showed predominant elastic modulus (∼930 Pa) at the interface.					
29438883	11	13	theme	gradient	1717:1724	arg1	378 ± 56 N					1733:1742	378 ± 56 N	1733:1742	378 ± 56 N	1733:1742	Further, biomechanical push-out studies showed significantly higher load for gradient group (378 ± 56 N) compared to others.					
29438883	11	13	theme	gradient	1717:1724	arg1	group					1726:1730	gradient group	1717:1730	gradient group (378 ± 56 N)	1717:1743	Further, biomechanical push-out studies showed significantly higher load for gradient group (378 ± 56 N) compared to others.					
29438883	10	14	theme	neo-tissue	1474:1483	arg1	formation					1485:1493	mineralized neo-tissue formation	1462:1493	mineralized neo-tissue formation	1462:1493	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	10	15	from	density	1558:1564	arg1	group					1633:1637	gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group	1569:1637	gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group	1569:1637	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	12	16	theme	host-tissue	1903:1913	arg1	integration					1915:1925	lateral host-tissue integration	1895:1925	lateral host-tissue integration	1895:1925	Thus, the developed nano-engineered gradient hydrogel enhanced hyaline cartilage regeneration with subchondral bone formation and lateral host-tissue integration.					
29438883	12	17	theme	subchondral	1864:1874	arg1	formation					1881:1889	subchondral bone formation	1864:1889	subchondral bone formation	1864:1889	Thus, the developed nano-engineered gradient hydrogel enhanced hyaline cartilage regeneration with subchondral bone formation and lateral host-tissue integration.					
29438883	8	18	theme	defect	1204:1209	arg1	closure					1193:1199	Complete closure	1184:1199	Complete closure of defect	1184:1209	Complete closure of defect was observed in gradient (8 weeks) while defect remained in other groups.					
29438883	6	19	theme	cells	958:962	arg1	retention					945:953	layer-specific retention	930:953	layer-specific retention of cells	930:962	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	6	19	theme	cells	958:962	arg1	interaction					978:988	cell-cell interaction	968:988	cell-cell interaction at the interface	968:1005	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	10	20	theme	mineralized	1462:1472	arg1	formation					1485:1493	mineralized neo-tissue formation	1462:1493	mineralized neo-tissue formation	1462:1493	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	5	21	theme	nHA	766:768	arg1	gradation					770:778	nHA gradation	766:778	nHA gradation	766:778	Microcomputed tomography (μCT) demonstrated nHA gradation while rheology showed predominant elastic modulus (∼930 Pa) at the interface.					
29438883	7	22	dep	biphasic	1046:1053	arg1	nHA					1056:1058	nHA	1056:1058	nHA	1056:1058	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels was compared with control using rabbit osteochondral defect after 3 and 8 weeks.					
29438883	7	22	dep	biphasic	1046:1053	arg1	ChS					1063:1065	ChS	1063:1065	ChS	1063:1065	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels was compared with control using rabbit osteochondral defect after 3 and 8 weeks.					
29438883	7	23	theme	In vivo	1008:1014	arg1	regeneration					1030:1041	In vivo osteochondral regeneration	1008:1041	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels	1008:1101	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels was compared with control using rabbit osteochondral defect after 3 and 8 weeks.					
29438883	3	24	theme	alkaline	549:556	arg1	phosphatase					558:568	alkaline phosphatase	549:568	alkaline phosphatase activity (p < 0.05)	549:588	Mineralized subchondral hydrogel exhibited significantly higher osteoblast proliferation and alkaline phosphatase activity (p < 0.05).					
29438883	3	25	theme	Mineralized	456:466	arg1	hydrogel					480:487	Mineralized subchondral hydrogel	456:487	Mineralized subchondral hydrogel	456:487	Mineralized subchondral hydrogel exhibited significantly higher osteoblast proliferation and alkaline phosphatase activity (p < 0.05).					
29438883	4	26	theme	Osteochondral	591:603	arg1	hydrogel					605:612	Osteochondral hydrogel	591:612	Osteochondral hydrogel	591:612	Osteochondral hydrogel exhibited interconnected porous structure and spatial variation with gradient interface of nHA and ChS-NPs.					
29438883	12	27	theme	nano-engineered	1785:1799	arg1	hydrogel					1810:1817	the developed nano-engineered gradient hydrogel	1771:1817	the developed nano-engineered gradient hydrogel	1771:1817	Thus, the developed nano-engineered gradient hydrogel enhanced hyaline cartilage regeneration with subchondral bone formation and lateral host-tissue integration.					
29438883	7	28	theme	osteochondral	1016:1028	arg1	regeneration					1030:1041	In vivo osteochondral regeneration	1008:1041	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels	1008:1101	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels was compared with control using rabbit osteochondral defect after 3 and 8 weeks.					
29438883	3	29	theme	osteoblast	520:529	arg1	proliferation					531:543	significantly higher osteoblast proliferation	499:543	significantly higher osteoblast proliferation	499:543	Mineralized subchondral hydrogel exhibited significantly higher osteoblast proliferation and alkaline phosphatase activity (p < 0.05).					
29438883	3	30	theme	subchondral	468:478	arg1	hydrogel					480:487	Mineralized subchondral hydrogel	456:487	Mineralized subchondral hydrogel	456:487	Mineralized subchondral hydrogel exhibited significantly higher osteoblast proliferation and alkaline phosphatase activity (p < 0.05).					
29438883	9	31	theme	glycosaminoglycan	1321:1337	arg1	deposition					1339:1348	collagen and glycosaminoglycan deposition	1308:1348	deposition	1339:1348	Histology demonstrated collagen and glycosaminoglycan deposition in neo-matrix and presence of hyaline cartilage-characteristic matrix, chondrocytes and osteoblasts.					
29438883	6	32	from	Co-culture	858:867	arg1	hydrogels					913:921	gradient hydrogels	904:921	gradient hydrogels	904:921	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	6	33	theme	layer-specific	930:943	arg1	retention					945:953	layer-specific retention	930:953	layer-specific retention of cells	930:962	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	1	34	theme	anisotropic	107:117	arg1	scaffold					141:148	anisotropic osteochondral-mimetic scaffold	107:148	anisotropic osteochondral-mimetic scaffold	107:148	Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging.					
29438883	12	35	theme	hyaline	1828:1834	arg1	cartilage					1836:1844	hyaline cartilage	1828:1844	hyaline cartilage regeneration	1828:1857	Thus, the developed nano-engineered gradient hydrogel enhanced hyaline cartilage regeneration with subchondral bone formation and lateral host-tissue integration.					
29438883	10	36	with	defect	1526:1531	arg1	density					1558:1564	higher bone mineral density	1538:1564	higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group	1538:1637	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	4	37	theme	gradient	683:690	arg1	interface					692:700	gradient interface	683:700	gradient interface of nHA and ChS-NPs	683:719	Osteochondral hydrogel exhibited interconnected porous structure and spatial variation with gradient interface of nHA and ChS-NPs.					
29438883	12	38	theme	gradient	1801:1808	arg1	hydrogel					1810:1817	the developed nano-engineered gradient hydrogel	1771:1817	the developed nano-engineered gradient hydrogel	1771:1817	Thus, the developed nano-engineered gradient hydrogel enhanced hyaline cartilage regeneration with subchondral bone formation and lateral host-tissue integration.					
29438883	5	39	theme	Microcomputed	722:734	arg1	μCT					748:750	μCT	748:750	μCT	748:750	Microcomputed tomography (μCT) demonstrated nHA gradation while rheology showed predominant elastic modulus (∼930 Pa) at the interface.					
29438883	5	39	theme	Microcomputed	722:734	arg1	tomography					736:745	Microcomputed tomography	722:745	Microcomputed tomography (μCT)	722:751	Microcomputed tomography (μCT) demonstrated nHA gradation while rheology showed predominant elastic modulus (∼930 Pa) at the interface.					
29438883	1	40	theme	osteochondral-mimetic	119:139	arg1	scaffold					141:148	anisotropic osteochondral-mimetic scaffold	107:148	anisotropic osteochondral-mimetic scaffold	107:148	Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging.					
29438883	0	41	theme	in	25:26	arg1	hydrogel					51:58	in situ forming composite hydrogel	25:58	in situ forming composite hydrogel for osteochondral regeneration	25:89	Gradient nano-engineered in situ forming composite hydrogel for osteochondral regeneration.					
29438883	4	42	theme	nHA	705:707	arg1	interface					692:700	gradient interface	683:700	gradient interface of nHA and ChS-NPs	683:719	Osteochondral hydrogel exhibited interconnected porous structure and spatial variation with gradient interface of nHA and ChS-NPs.					
29438883	9	43	theme	hyaline	1380:1386	arg1	matrix					1413:1418	hyaline cartilage-characteristic matrix	1380:1418	hyaline cartilage-characteristic matrix	1380:1418	Histology demonstrated collagen and glycosaminoglycan deposition in neo-matrix and presence of hyaline cartilage-characteristic matrix, chondrocytes and osteoblasts.					
29438883	1	44	theme	scaffold	141:148	arg1	Fabrication					92:102	Fabrication	92:102	Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface	92:205	Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging.					
29438883	0	45	theme	composite	41:49	arg1	hydrogel					51:58	in situ forming composite hydrogel	25:58	in situ forming composite hydrogel for osteochondral regeneration	25:89	Gradient nano-engineered in situ forming composite hydrogel for osteochondral regeneration.					
29438883	2	46	with	construct	299:307	arg1	nHA					382:384	nHA	382:384	nHA	382:384	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	2	46	with	construct	299:307	arg1	∼30-90 nm					388:396	∼30-90 nm	388:396	∼30-90 nm	388:396	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	2	46	with	construct	299:307	arg1	nanohydroxyapatite					362:379	nanohydroxyapatite	362:379	nanohydroxyapatite (nHA) (∼30-90 nm)	362:397	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	2	46	with	construct	299:307	arg1	nanoparticles					334:346	chondroitin sulfate nanoparticles	314:346	chondroitin sulfate nanoparticles (ChS-NPs)	314:356	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	2	46	with	construct	299:307	arg1	ChS-NPs					349:355	ChS-NPs	349:355	ChS-NPs	349:355	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	9	47	theme	cartilage-characteristic	1388:1411	arg1	matrix					1413:1418	hyaline cartilage-characteristic matrix	1380:1418	hyaline cartilage-characteristic matrix	1380:1418	Histology demonstrated collagen and glycosaminoglycan deposition in neo-matrix and presence of hyaline cartilage-characteristic matrix, chondrocytes and osteoblasts.					
29438883	12	48	theme	lateral	1895:1901	arg1	integration					1915:1925	lateral host-tissue integration	1895:1925	lateral host-tissue integration	1895:1925	Thus, the developed nano-engineered gradient hydrogel enhanced hyaline cartilage regeneration with subchondral bone formation and lateral host-tissue integration.					
29438883	11	49	theme	push-out	1663:1670	arg1	studies					1672:1678	biomechanical push-out studies	1649:1678	biomechanical push-out studies	1649:1678	Further, biomechanical push-out studies showed significantly higher load for gradient group (378 ± 56 N) compared to others.					
29438883	3	50	theme	phosphatase	558:568	arg1	activity					570:577	alkaline phosphatase activity	549:577	alkaline phosphatase activity (p < 0.05)	549:588	Mineralized subchondral hydrogel exhibited significantly higher osteoblast proliferation and alkaline phosphatase activity (p < 0.05).					
29438883	3	50	theme	phosphatase	558:568	arg1	p < 0.05					580:587	p < 0.05	580:587	p < 0.05	580:587	Mineralized subchondral hydrogel exhibited significantly higher osteoblast proliferation and alkaline phosphatase activity (p < 0.05).					
29438883	0	51	theme	forming	33:39	arg1	hydrogel					51:58	in situ forming composite hydrogel	25:58	in situ forming composite hydrogel for osteochondral regeneration	25:89	Gradient nano-engineered in situ forming composite hydrogel for osteochondral regeneration.					
29438883	4	52	theme	spatial	660:666	arg1	variation					668:676	spatial variation	660:676	spatial variation	660:676	Osteochondral hydrogel exhibited interconnected porous structure and spatial variation with gradient interface of nHA and ChS-NPs.					
29438883	1	53	with	Fabrication	92:102	arg1	zone					179:182	mineralized subchondral zone	155:182	mineralized subchondral zone	155:182	Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging.					
29438883	1	53	with	Fabrication	92:102	arg1	interface					197:205	gradient interface	188:205	gradient interface	188:205	Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging.					
29438883	9	54	theme	matrix	1413:1418	arg1	presence					1368:1375	presence	1368:1375	presence	1368:1375	Histology demonstrated collagen and glycosaminoglycan deposition in neo-matrix and presence of hyaline cartilage-characteristic matrix, chondrocytes and osteoblasts.					
29438883	9	54	theme	matrix	1413:1418	arg1	neo-matrix					1353:1362	neo-matrix	1353:1362	neo-matrix	1353:1362	Histology demonstrated collagen and glycosaminoglycan deposition in neo-matrix and presence of hyaline cartilage-characteristic matrix, chondrocytes and osteoblasts.					
29438883	8	55	located	observed	1215:1222	arg2	closure					1193:1199	Complete closure	1184:1199	Complete closure of defect	1184:1209	Complete closure of defect was observed in gradient (8 weeks) while defect remained in other groups.					
29438883	8	55	located	observed	1215:1222	arg1	gradient					1227:1234	gradient	1227:1234	gradient	1227:1234	Complete closure of defect was observed in gradient (8 weeks) while defect remained in other groups.					
29438883	12	56	theme	developed	1775:1783	arg1	hydrogel					1810:1817	the developed nano-engineered gradient hydrogel	1771:1817	the developed nano-engineered gradient hydrogel	1771:1817	Thus, the developed nano-engineered gradient hydrogel enhanced hyaline cartilage regeneration with subchondral bone formation and lateral host-tissue integration.					
29438883	2	57	theme	hydrogel	290:297	arg1	construct					299:307	an injectable semi-interpenetrating network hydrogel construct	246:307	an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm)	246:397	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	6	58	theme	gradient	904:911	arg1	hydrogels					913:921	gradient hydrogels	904:921	gradient hydrogels	904:921	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	2	59	theme	chondral	402:409	arg1	zones					436:440	chondral and subchondral hydrogel zones	402:440	chondral and subchondral hydrogel zones respectively	402:453	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	6	60	from	interface	997:1005	arg1	retention					945:953	layer-specific retention	930:953	layer-specific retention of cells	930:962	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	6	60	from	interface	997:1005	arg1	interaction					978:988	cell-cell interaction	968:988	cell-cell interaction at the interface	968:1005	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	10	61	theme	gradient	1569:1576	arg1	group					1633:1637	gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group	1569:1637	gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group	1569:1637	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	4	62	theme	interconnected	624:637	arg1	structure					646:654	interconnected porous structure	624:654	interconnected porous structure	624:654	Osteochondral hydrogel exhibited interconnected porous structure and spatial variation with gradient interface of nHA and ChS-NPs.					
29438883	2	63	theme	semi-interpenetrating	260:280	arg1	construct					299:307	an injectable semi-interpenetrating network hydrogel construct	246:307	an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm)	246:397	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	2	64	theme	network	282:288	arg1	construct					299:307	an injectable semi-interpenetrating network hydrogel construct	246:307	an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm)	246:397	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	1	65	theme	mineralized	155:165	arg1	zone					179:182	mineralized subchondral zone	155:182	mineralized subchondral zone	155:182	Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging.					
29438883	2	66	theme	injectable	249:258	arg1	construct					299:307	an injectable semi-interpenetrating network hydrogel construct	246:307	an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm)	246:397	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	6	67	theme	chondrocytes	888:899	arg1	Co-culture					858:867	Co-culture	858:867	Co-culture of osteoblasts and chondrocytes in gradient hydrogels	858:921	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	1	68	theme	subchondral	167:177	arg1	zone					179:182	mineralized subchondral zone	155:182	mineralized subchondral zone	155:182	Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging.					
29438883	7	69	theme	gradient	1072:1079	arg1	hydrogels					1093:1101	biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels	1046:1101	hydrogels	1093:1101	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels was compared with control using rabbit osteochondral defect after 3 and 8 weeks.					
29438883	7	69	theme	gradient	1072:1079	arg1	nHA + ChS					1082:1090	nHA + ChS	1082:1090	nHA + ChS	1082:1090	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels was compared with control using rabbit osteochondral defect after 3 and 8 weeks.					
29438883	0	70	dep	in	25:26	arg1	situ					28:31	situ	28:31	situ	28:31	Gradient nano-engineered in situ forming composite hydrogel for osteochondral regeneration.					
29438883	7	71	theme	osteochondral	1142:1154	arg1	defect					1156:1161	rabbit osteochondral defect	1135:1161	rabbit osteochondral defect	1135:1161	In vivo osteochondral regeneration by biphasic (nHA or ChS) and gradient (nHA + ChS) hydrogels was compared with control using rabbit osteochondral defect after 3 and 8 weeks.					
29438883	1	72	theme	gradient	188:195	arg1	interface					197:205	gradient interface	188:205	gradient interface	188:205	Fabrication of anisotropic osteochondral-mimetic scaffold with mineralized subchondral zone and gradient interface remains challenging.					
29438883	11	73	theme	biomechanical	1649:1661	arg1	studies					1672:1678	biomechanical push-out studies	1649:1678	biomechanical push-out studies	1649:1678	Further, biomechanical push-out studies showed significantly higher load for gradient group (378 ± 56 N) compared to others.					
29438883	8	74	dep	observed	1215:1222	arg1	weeks					1239:1243	8 weeks	1237:1243	8 weeks	1237:1243	Complete closure of defect was observed in gradient (8 weeks) while defect remained in other groups.					
29438883	10	75	theme	bone	1545:1548	arg1	density					1558:1564	higher bone mineral density	1538:1564	higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group	1538:1637	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	2	76	theme	sulfate	326:332	arg1	ChS-NPs					349:355	ChS-NPs	349:355	ChS-NPs	349:355	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	2	76	theme	sulfate	326:332	arg1	nanoparticles					334:346	chondroitin sulfate nanoparticles	314:346	chondroitin sulfate nanoparticles (ChS-NPs)	314:356	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	6	77	theme	osteoblasts	872:882	arg1	Co-culture					858:867	Co-culture	858:867	Co-culture of osteoblasts and chondrocytes in gradient hydrogels	858:921	Co-culture of osteoblasts and chondrocytes in gradient hydrogels showed layer-specific retention of cells and cell-cell interaction at the interface.					
29438883	10	78	dep	chondral	1579:1586	arg1	0.64 ± 0.08 g/cc					1615:1630	0.64 ± 0.08 g/cc	1615:1630	0.64 ± 0.08 g/cc	1615:1630	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	10	78	dep	chondral	1579:1586	arg1	osteal					1607:1612	osteal	1607:1612	osteal	1607:1612	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	10	78	dep	chondral	1579:1586	arg1	0.42 ± 0.07 g/cc					1589:1604	0.42 ± 0.07 g/cc	1589:1604	0.42 ± 0.07 g/cc	1589:1604	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	0	79	theme	osteochondral	64:76	arg1	regeneration					78:89	osteochondral regeneration	64:89	osteochondral regeneration	64:89	Gradient nano-engineered in situ forming composite hydrogel for osteochondral regeneration.					
29438883	3	80	theme	higher	513:518	arg1	proliferation					531:543	significantly higher osteoblast proliferation	499:543	significantly higher osteoblast proliferation	499:543	Mineralized subchondral hydrogel exhibited significantly higher osteoblast proliferation and alkaline phosphatase activity (p < 0.05).					
29438883	2	81	theme	chondroitin	314:324	arg1	sulfate					326:332	chondroitin sulfate	314:332	chondroitin sulfate nanoparticles (ChS-NPs)	314:356	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	10	82	theme	higher	1538:1543	arg1	density					1558:1564	higher bone mineral density	1538:1564	higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group	1538:1637	μCT showed mineralized neo-tissue formation, which was confined within the defect with higher bone mineral density in gradient (chondral: 0.42 ± 0.07 g/cc, osteal: 0.64 ± 0.08 g/cc) group.					
29438883	2	83	theme	hydrogel	427:434	arg1	zones					436:440	chondral and subchondral hydrogel zones	402:440	chondral and subchondral hydrogel zones respectively	402:453	We have developed an injectable semi-interpenetrating network hydrogel construct with chondroitin sulfate nanoparticles (ChS-NPs) and nanohydroxyapatite (nHA) (∼30-90 nm) in chondral and subchondral hydrogel zones respectively.					
29438883	11	84	theme	higher	1701:1706	arg1	load					1708:1711	significantly higher load	1687:1711	significantly higher load for gradient group (378 ± 56 N)	1687:1743	Further, biomechanical push-out studies showed significantly higher load for gradient group (378 ± 56 N) compared to others.					
31320078	8	0	from	purifier	1148:1155	arg1	treatment					1167:1175	sewage treatment	1160:1175	sewage treatment	1160:1175	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	5	1	theme	uptake	628:633	arg1	ability					635:641	significant uptake ability	616:641	significant uptake ability	616:641	Typically, the sample showed significant uptake ability towards MB and TC with uptake characteristics of pseudo-second-order model and Langmuir isotherm model.					
31320078	7	2	theme	consecutive	971:981	arg1	recycles					988:995	consecutive four recycles	971:995	consecutive four recycles	971:995	Meanwhile, the sample was ease of regeneration and kept stable reusability through consecutive four recycles.					
31320078	2	3	theme	complex	338:344	arg1	wastewater					346:355	complex wastewater	338:355	complex wastewater	338:355	Here, we used an organic-inorganic material, i.e. bacterial cellulose (BC) and Ca-montmorillonite (Ca-MMT) composites to treat complex wastewater.					
31320078	8	4	theme	raw	1073:1075	arg1	materials					1077:1085	the two raw materials	1065:1085	the two raw materials	1065:1085	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	0	5	theme	efficient	82:90	arg1	removal					92:98	efficient removal	82:98	efficient removal of dyes and antibiotics	82:122	Handy purifier based on bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics.					
31320078	4	6	theme	superior	508:515	arg1	efficiency					525:534	superior removal efficiency	508:534	superior removal efficiency towards methylene blue (MB) and tetracycline (TC)	508:584	We demonstrated the BC/Ca-MMT has superior removal efficiency towards methylene blue (MB) and tetracycline (TC).					
31320078	3	7	dep	surface	362:368	arg1	The					358:360	The	358:360	The	358:360	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	7	8	theme	stable	944:949	arg1	reusability					951:961	kept stable reusability	939:961	kept stable reusability through consecutive four recycles	939:995	Meanwhile, the sample was ease of regeneration and kept stable reusability through consecutive four recycles.					
31320078	1	9	theme	imperative	164:173	arg1	issue					191:195	an imperative but challenging issue	161:195	an imperative but challenging issue of our times	161:208	Water purification has always being an imperative but challenging issue of our times.					
31320078	1	9	theme	imperative	164:173	arg1	purification					131:142	Water purification	125:142	Water purification	125:142	Water purification has always being an imperative but challenging issue of our times.					
31320078	0	10	theme	dyes	103:106	arg1	removal					92:98	efficient removal	82:98	efficient removal of dyes and antibiotics	82:122	Handy purifier based on bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics.					
31320078	7	11	theme	regeneration	922:933	arg1	sample					903:908	the sample	899:908	the sample	899:908	Meanwhile, the sample was ease of regeneration and kept stable reusability through consecutive four recycles.					
31320078	7	11	theme	regeneration	922:933	arg1	ease					914:917	ease	914:917	ease of regeneration	914:933	Meanwhile, the sample was ease of regeneration and kept stable reusability through consecutive four recycles.					
31320078	7	11	theme	regeneration	922:933	arg1	reusability					951:961	kept stable reusability	939:961	kept stable reusability through consecutive four recycles	939:995	Meanwhile, the sample was ease of regeneration and kept stable reusability through consecutive four recycles.					
31320078	8	12	theme	low	1025:1027	arg1	cost					1029:1032	low cost	1025:1032	low cost	1025:1032	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	5	13	theme	Langmuir	722:729	arg1	model					740:744	Langmuir isotherm model	722:744	Langmuir isotherm model	722:744	Typically, the sample showed significant uptake ability towards MB and TC with uptake characteristics of pseudo-second-order model and Langmuir isotherm model.					
31320078	0	14	theme	antibiotics	112:122	arg1	removal					92:98	efficient removal	82:98	efficient removal of dyes and antibiotics	82:122	Handy purifier based on bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics.					
31320078	8	15	theme	promising	1122:1130	arg1	purifier					1148:1155	a promising versatile water purifier	1120:1155	a promising versatile water purifier in sewage treatment	1120:1175	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	8	15	theme	promising	1122:1130	arg1	BC/Ca-MMT					1092:1100	the BC/Ca-MMT	1088:1100	the BC/Ca-MMT	1088:1100	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	3	16	theme	adsorption	456:465	arg1	sites					467:471	abundant adsorption sites	447:471	abundant adsorption sites	447:471	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	5	17	theme	model	740:744	arg1	characteristics					673:687	uptake characteristics	666:687	uptake characteristics of pseudo-second-order model and Langmuir isotherm model	666:744	Typically, the sample showed significant uptake ability towards MB and TC with uptake characteristics of pseudo-second-order model and Langmuir isotherm model.					
31320078	6	18	theme	coexisting	865:874	arg1	components					876:885	other coexisting components	859:885	other coexisting components	859:885	More interestingly, in MB-TC binary system, the removal of the two contaminative species was hardly affected by other coexisting components.					
31320078	3	19	theme	BC/Ca-MMT	388:396	arg1	structure					416:424	a microporous structure	402:424	a microporous structure capable of providing abundant adsorption sites	402:471	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	3	19	theme	BC/Ca-MMT	388:396	arg1	inside					374:379	inside	374:379	inside	374:379	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	3	19	theme	BC/Ca-MMT	388:396	arg1	surface					362:368	surface	362:368	surface	362:368	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	2	20	theme	bacterial	261:269	arg1	BC					282:283	BC	282:283	BC	282:283	Here, we used an organic-inorganic material, i.e. bacterial cellulose (BC) and Ca-montmorillonite (Ca-MMT) composites to treat complex wastewater.					
31320078	2	20	theme	bacterial	261:269	arg1	cellulose					271:279	bacterial cellulose	261:279	bacterial cellulose (BC)	261:284	Here, we used an organic-inorganic material, i.e. bacterial cellulose (BC) and Ca-montmorillonite (Ca-MMT) composites to treat complex wastewater.					
31320078	1	21	theme	challenging	179:189	arg1	issue					191:195	an imperative but challenging issue	161:195	an imperative but challenging issue of our times	161:208	Water purification has always being an imperative but challenging issue of our times.					
31320078	1	21	theme	challenging	179:189	arg1	purification					131:142	Water purification	125:142	Water purification	125:142	Water purification has always being an imperative but challenging issue of our times.					
31320078	8	22	theme	water	1142:1146	arg1	purifier					1148:1155	a promising versatile water purifier	1120:1155	a promising versatile water purifier in sewage treatment	1120:1175	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	8	22	theme	water	1142:1146	arg1	BC/Ca-MMT					1092:1100	the BC/Ca-MMT	1088:1100	the BC/Ca-MMT	1088:1100	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	6	23	theme	other	859:863	arg1	components					876:885	other coexisting components	859:885	other coexisting components	859:885	More interestingly, in MB-TC binary system, the removal of the two contaminative species was hardly affected by other coexisting components.					
31320078	6	24	theme	binary	776:781	arg1	system					783:788	MB-TC binary system	770:788	MB-TC binary system	770:788	More interestingly, in MB-TC binary system, the removal of the two contaminative species was hardly affected by other coexisting components.					
31320078	0	25	theme	Handy	0:4	arg1	purifier					6:13	Handy purifier	0:13	Handy purifier	0:13	Handy purifier based on bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics.					
31320078	5	26	theme	significant	616:626	arg1	ability					635:641	significant uptake ability	616:641	significant uptake ability	616:641	Typically, the sample showed significant uptake ability towards MB and TC with uptake characteristics of pseudo-second-order model and Langmuir isotherm model.					
31320078	6	27	theme	MB-TC	770:774	arg1	system					783:788	MB-TC binary system	770:788	MB-TC binary system	770:788	More interestingly, in MB-TC binary system, the removal of the two contaminative species was hardly affected by other coexisting components.					
31320078	3	28	theme	microporous	404:414	arg1	structure					416:424	a microporous structure	402:424	a microporous structure capable of providing abundant adsorption sites	402:471	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	3	28	theme	microporous	404:414	arg1	inside					374:379	inside	374:379	inside	374:379	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	3	28	theme	microporous	404:414	arg1	surface					362:368	surface	362:368	surface	362:368	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	4	29	theme	blue	554:557	arg1	methylene					544:552	methylene blue	544:557	methylene blue (MB)	544:562	We demonstrated the BC/Ca-MMT has superior removal efficiency towards methylene blue (MB) and tetracycline (TC).					
31320078	4	29	theme	blue	554:557	arg1	MB					560:561	MB	560:561	MB	560:561	We demonstrated the BC/Ca-MMT has superior removal efficiency towards methylene blue (MB) and tetracycline (TC).					
31320078	8	30	theme	resources	1052:1060	arg1	cost					1029:1032	low cost	1025:1032	low cost	1025:1032	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	8	30	theme	resources	1052:1060	arg1	range					1043:1047	wide range	1038:1047	wide range	1038:1047	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	8	31	theme	more	1003:1006	arg1	range					1043:1047	wide range	1038:1047	wide range	1038:1047	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	8	31	theme	more	1003:1006	arg1	cost					1029:1032	low cost	1025:1032	low cost	1025:1032	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	8	31	theme	more	1003:1006	arg1	virtues					1008:1014	more virtues	1003:1014	more virtues	1003:1014	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	3	32	theme	abundant	447:454	arg1	sites					467:471	abundant adsorption sites	447:471	abundant adsorption sites	447:471	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	2	33	used	used	220:223	arg2	we					217:218	we	217:218	we	217:218	Here, we used an organic-inorganic material, i.e. bacterial cellulose (BC) and Ca-montmorillonite (Ca-MMT) composites to treat complex wastewater.					
31320078	1	34	theme	times	204:208	arg1	issue					191:195	an imperative but challenging issue	161:195	an imperative but challenging issue of our times	161:208	Water purification has always being an imperative but challenging issue of our times.					
31320078	1	34	theme	times	204:208	arg1	purification					131:142	Water purification	125:142	Water purification	125:142	Water purification has always being an imperative but challenging issue of our times.					
31320078	0	35	theme	cellulose	34:42	arg1	composites					67:76	bacterial cellulose and Ca-montmorillonite composites	24:76	bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics	24:122	Handy purifier based on bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics.					
31320078	5	36	theme	pseudo-second-order	692:710	arg1	model					712:716	pseudo-second-order model	692:716	pseudo-second-order model	692:716	Typically, the sample showed significant uptake ability towards MB and TC with uptake characteristics of pseudo-second-order model and Langmuir isotherm model.					
31320078	0	37	theme	bacterial	24:32	arg1	composites					67:76	bacterial cellulose and Ca-montmorillonite composites	24:76	bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics	24:122	Handy purifier based on bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics.					
31320078	5	38	theme	model	712:716	arg1	characteristics					673:687	uptake characteristics	666:687	uptake characteristics of pseudo-second-order model and Langmuir isotherm model	666:744	Typically, the sample showed significant uptake ability towards MB and TC with uptake characteristics of pseudo-second-order model and Langmuir isotherm model.					
31320078	0	39	theme	Ca-montmorillonite	48:65	arg1	composites					67:76	bacterial cellulose and Ca-montmorillonite composites	24:76	bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics	24:122	Handy purifier based on bacterial cellulose and Ca-montmorillonite composites for efficient removal of dyes and antibiotics.					
31320078	4	40	theme	removal	517:523	arg1	efficiency					525:534	superior removal efficiency	508:534	superior removal efficiency towards methylene blue (MB) and tetracycline (TC)	508:584	We demonstrated the BC/Ca-MMT has superior removal efficiency towards methylene blue (MB) and tetracycline (TC).					
31320078	8	41	theme	sewage	1160:1165	arg1	treatment					1167:1175	sewage treatment	1160:1175	sewage treatment	1160:1175	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	4	42	dep	BC/Ca-MMT	494:502	arg1	has					504:506	has	504:506	has superior removal efficiency towards methylene blue (MB) and tetracycline (TC)	504:584	We demonstrated the BC/Ca-MMT has superior removal efficiency towards methylene blue (MB) and tetracycline (TC).					
31320078	6	43	theme	species	828:834	arg1	removal					795:801	the removal	791:801	the removal of the two contaminative species	791:834	More interestingly, in MB-TC binary system, the removal of the two contaminative species was hardly affected by other coexisting components.					
31320078	1	44	theme	Water	125:129	arg1	purification					131:142	Water purification	125:142	Water purification	125:142	Water purification has always being an imperative but challenging issue of our times.					
31320078	1	44	theme	Water	125:129	arg1	issue					191:195	an imperative but challenging issue	161:195	an imperative but challenging issue of our times	161:208	Water purification has always being an imperative but challenging issue of our times.					
31320078	6	45	theme	contaminative	814:826	arg1	species					828:834	the two contaminative species	806:834	the two contaminative species	806:834	More interestingly, in MB-TC binary system, the removal of the two contaminative species was hardly affected by other coexisting components.					
31320078	8	46	theme	materials	1077:1085	arg1	cost					1029:1032	low cost	1025:1032	low cost	1025:1032	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	8	46	theme	materials	1077:1085	arg1	range					1043:1047	wide range	1038:1047	wide range	1038:1047	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	5	47	theme	isotherm	731:738	arg1	model					740:744	Langmuir isotherm model	722:744	Langmuir isotherm model	722:744	Typically, the sample showed significant uptake ability towards MB and TC with uptake characteristics of pseudo-second-order model and Langmuir isotherm model.					
31320078	5	48	theme	uptake	666:671	arg1	characteristics					673:687	uptake characteristics	666:687	uptake characteristics of pseudo-second-order model and Langmuir isotherm model	666:744	Typically, the sample showed significant uptake ability towards MB and TC with uptake characteristics of pseudo-second-order model and Langmuir isotherm model.					
31320078	8	49	theme	versatile	1132:1140	arg1	purifier					1148:1155	a promising versatile water purifier	1120:1155	a promising versatile water purifier in sewage treatment	1120:1175	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	8	49	theme	versatile	1132:1140	arg1	BC/Ca-MMT					1092:1100	the BC/Ca-MMT	1088:1100	the BC/Ca-MMT	1088:1100	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	8	50	theme	wide	1038:1041	arg1	range					1043:1047	wide range	1038:1047	wide range	1038:1047	With more virtues, such as low cost and wide range of resources of the two raw materials, the BC/Ca-MMT is expected to be a promising versatile water purifier in sewage treatment.					
31320078	2	51	dep	cellulose	271:279	arg1	composites					318:327	composites	318:327	composites	318:327	Here, we used an organic-inorganic material, i.e. bacterial cellulose (BC) and Ca-montmorillonite (Ca-MMT) composites to treat complex wastewater.					
31320078	2	51	dep	cellulose	271:279	arg1	i.e.					256:259	i.e.	256:259	i.e.	256:259	Here, we used an organic-inorganic material, i.e. bacterial cellulose (BC) and Ca-montmorillonite (Ca-MMT) composites to treat complex wastewater.					
31320078	3	52	theme	capable	426:432	arg1	structure					416:424	a microporous structure	402:424	a microporous structure capable of providing abundant adsorption sites	402:471	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	3	52	theme	capable	426:432	arg1	inside					374:379	inside	374:379	inside	374:379	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	3	52	theme	capable	426:432	arg1	surface					362:368	surface	362:368	surface	362:368	The surface and inside of the BC/Ca-MMT was a microporous structure capable of providing abundant adsorption sites.					
31320078	2	53	theme	organic-inorganic	228:244	arg1	material					246:253	an organic-inorganic material	225:253	an organic-inorganic material	225:253	Here, we used an organic-inorganic material, i.e. bacterial cellulose (BC) and Ca-montmorillonite (Ca-MMT) composites to treat complex wastewater.					
30719912	0	0	theme	Lipopolysaccharide-Peptide	74:99	arg1	Interaction					101:111	That Involved Lipopolysaccharide-Peptide Interaction	60:111	That Involved Lipopolysaccharide-Peptide Interaction	60:111	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide-Peptide Interaction.					
30719912	4	1	theme	fatty	768:772	arg1	SCFAs					781:785	SCFAs	781:785	SCFAs	781:785	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	4	1	theme	fatty	768:772	arg1	acids					774:778	short-chain fatty acids	756:778	short-chain fatty acids (SCFAs)	756:786	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	2	2	theme	7S	385:386	arg1	globulin					388:395	soybean 7S globulin	377:395	soybean 7S globulin on gut microbiota	377:413	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	0	3	theme	Involved	65:72	arg1	Interaction					101:111	That Involved Lipopolysaccharide-Peptide Interaction	60:111	That Involved Lipopolysaccharide-Peptide Interaction	60:111	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide-Peptide Interaction.					
30719912	1	4	theme	metabolic	183:191	arg1	syndrome					193:200	metabolic syndrome	183:200	metabolic syndrome	183:200	Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders.					
30719912	4	5	theme	relative	832:839	arg1	abundance					841:849	the relative abundance	828:849	the relative abundance of Lachnospiraceae and Lactobacillaceae	828:889	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	6	6	from	improvement	1198:1208	arg1	disease					1261:1267	metabolic disease	1251:1267	metabolic disease	1251:1267	This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.					
30719912	6	7	theme	globulin	1239:1246	arg1	effects					1217:1223	the effects	1213:1223	the effects of soybean 7S globulin on metabolic disease	1213:1267	This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.					
30719912	3	8	theme	extension	586:594	arg1	ER					604:605	ER	604:605	ER	604:605	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	3	8	theme	extension	586:594	arg1	region					596:601	extension region	586:601	the extension region (ER) fragments of soybean 7S globulin	582:639	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	6	9	theme	effects	1217:1223	arg1	improvement					1198:1208	improvement	1198:1208	improvement of the effects of soybean 7S globulin on metabolic disease	1198:1267	This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.					
30719912	1	10	theme	metabolic	278:286	arg1	disorders					288:296	metabolic disorders	278:296	metabolic disorders	278:296	Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders.					
30719912	6	11	theme	metabolic	1251:1259	arg1	disease					1261:1267	metabolic disease	1251:1267	metabolic disease	1251:1267	This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.					
30719912	3	12	theme	region	596:601	arg1	fragments					608:616	the extension region (ER) fragments	582:616	the extension region (ER) fragments of soybean 7S globulin	582:639	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	1	13	theme	disorders	288:296	arg1	control					267:273	the control	263:273	the control of metabolic disorders	263:296	Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders.					
30719912	4	14	theme	short-chain	756:766	arg1	SCFAs					781:785	SCFAs	781:785	SCFAs	781:785	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	4	14	theme	short-chain	756:766	arg1	acids					774:778	short-chain fatty acids	756:778	short-chain fatty acids (SCFAs)	756:786	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	2	15	from	peptides	365:372	arg1	microbiota					404:413	gut microbiota	400:413	gut microbiota	400:413	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	5	16	theme	Ca2+	1042:1045	arg1	presence					1030:1037	the presence	1026:1037	the presence of Ca2+	1026:1045	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	5	17	theme	Langmuir	935:942	arg1	studies					954:960	Langmuir monolayer studies	935:960	Langmuir monolayer studies	935:960	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	1	18	theme	syndrome	193:200	arg1	control					172:178	the control	168:178	the control of metabolic syndrome	168:200	Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders.					
30719912	2	19	theme	lipopolysaccharide	490:507	arg1	changes					448:454	changes	448:454	changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction	448:533	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	5	20	theme	ER	980:981	arg1	peptides					983:990	ER peptides	980:990	ER peptides	980:990	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	0	21	dep	Interaction	101:111	arg1	Modulation					0:9	Modulation	0:9	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide	0:58	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide-Peptide Interaction.					
30719912	6	22	from	disease	1261:1267	arg1	improvement					1198:1208	improvement	1198:1208	improvement of the effects of soybean 7S globulin on metabolic disease	1198:1267	This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.					
30719912	2	23	theme	-peptide	514:521	arg1	interaction					523:533	-peptide interaction	514:533	-peptide interaction	514:533	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	6	24	theme	7S	1236:1237	arg1	globulin					1239:1246	soybean 7S globulin	1228:1246	soybean 7S globulin	1228:1246	This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.					
30719912	4	25	theme	highest	734:740	arg1	production					742:751	the highest production	730:751	the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae	730:889	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	2	26	theme	composition	474:484	arg1	changes					448:454	changes	448:454	changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction	448:533	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	2	27	dep	composition	474:484	arg1	interaction					523:533	-peptide interaction	514:533	-peptide interaction	514:533	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	6	28	theme	soybean	1228:1234	arg1	globulin					1239:1246	soybean 7S globulin	1228:1246	soybean 7S globulin	1228:1246	This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.					
30719912	2	29	theme	gut	400:402	arg1	microbiota					404:413	gut microbiota	400:413	gut microbiota	400:413	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	2	30	theme	microbiota	463:472	arg1	composition					474:484	gut microbiota composition	459:484	gut microbiota composition	459:484	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	5	31	theme	β-sheet-rich	1066:1077	arg1	structures					1089:1098	β-sheet-rich aggregate structures	1066:1098	β-sheet-rich aggregate structures	1066:1098	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	2	32	from	globulin	388:395	arg1	microbiota					404:413	gut microbiota	400:413	gut microbiota	400:413	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	1	33	theme	Soybean	114:120	arg1	protein					122:128	Soybean protein	114:128	Soybean protein	114:128	Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders.					
30719912	4	34	theme	Lactobacillaceae	874:889	arg1	abundance					841:849	the relative abundance	828:849	the relative abundance of Lachnospiraceae and Lactobacillaceae	828:889	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	5	35	theme	titration	903:911	arg1	ITC					926:928	ITC	926:928	ITC	926:928	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	5	35	theme	titration	903:911	arg1	calorimetry					913:923	Isothermal titration calorimetry	892:923	Isothermal titration calorimetry (ITC)	892:929	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	2	36	theme	gut	459:461	arg1	composition					474:484	gut microbiota composition	459:484	gut microbiota composition	459:484	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	6	37	from	effects	1217:1223	arg1	disease					1261:1267	metabolic disease	1251:1267	metabolic disease	1251:1267	This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.					
30719912	5	38	theme	monolayers	1137:1146	arg1	stability					1120:1128	the stability	1116:1128	the stability of LPS monolayers	1116:1146	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	2	39	theme	soybean	377:383	arg1	globulin					388:395	soybean 7S globulin	377:395	soybean 7S globulin on gut microbiota	377:413	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	0	40	theme	Soybean	32:38	arg1	Peptide					52:58	Soybean 7S Globulin Peptide	32:58	Soybean 7S Globulin Peptide	32:58	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide-Peptide Interaction.					
30719912	2	41	theme	peptides	365:372	arg1	association					428:438	possible association	419:438	possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction	419:533	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	2	41	theme	peptides	365:372	arg1	modulation					335:344	the modulation	331:344	the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota	331:413	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	3	42	theme	7S	629:630	arg1	globulin					632:639	soybean 7S globulin	621:639	soybean 7S globulin	621:639	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	5	43	theme	monolayer	944:952	arg1	studies					954:960	Langmuir monolayer studies	935:960	Langmuir monolayer studies	935:960	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	1	44	theme	gut	230:232	arg1	microbiota					234:243	gut microbiota	230:243	gut microbiota	230:243	Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders.					
30719912	2	45	theme	possible	419:426	arg1	association					428:438	possible association	419:438	possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction	419:533	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	0	46	theme	Microbiota	18:27	arg1	Modulation					0:9	Modulation	0:9	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide	0:58	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide-Peptide Interaction.					
30719912	2	47	from	microbiota	404:413	arg1	peptides					365:372	pepsin-released peptides	349:372	pepsin-released peptides of soybean 7S globulin on gut microbiota	349:413	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	4	48	theme	ER	704:705	arg1	peptides					707:714	ER peptides	704:714	ER peptides	704:714	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	0	49	theme	Globulin	43:50	arg1	Peptide					52:58	Soybean 7S Globulin Peptide	32:58	Soybean 7S Globulin Peptide	32:58	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide-Peptide Interaction.					
30719912	5	50	theme	Isothermal	892:901	arg1	ITC					926:928	ITC	926:928	ITC	926:928	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	5	50	theme	Isothermal	892:901	arg1	calorimetry					913:923	Isothermal titration calorimetry	892:923	Isothermal titration calorimetry (ITC)	892:929	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	0	51	theme	7S	40:41	arg1	Peptide					52:58	Soybean 7S Globulin Peptide	32:58	Soybean 7S Globulin Peptide	32:58	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide-Peptide Interaction.					
30719912	3	52	theme	soybean	621:627	arg1	globulin					632:639	soybean 7S globulin	621:639	soybean 7S globulin	621:639	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	3	53	theme	fermentation	545:556	arg1	experiments					558:568	In vitro fermentation experiments	536:568	In vitro fermentation experiments	536:568	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	1	54	theme	nutritional	139:149	arg1	significance					151:162	nutritional significance	139:162	nutritional significance for the control of metabolic syndrome	139:200	Soybean protein exhibits nutritional significance for the control of metabolic syndrome, and evidence suggests that gut microbiota are implicated in the control of metabolic disorders.					
30719912	5	55	theme	aggregate	1079:1087	arg1	structures					1089:1098	β-sheet-rich aggregate structures	1066:1098	β-sheet-rich aggregate structures	1066:1098	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	4	56	theme	abundance	841:849	arg1	increase					816:823	increase	816:823	increase of the relative abundance of Lachnospiraceae and Lactobacillaceae	816:889	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	3	57	theme	proinflammatory	664:678	arg1	bacteria					694:701	proinflammatory Gram-negative bacteria	664:701	proinflammatory Gram-negative bacteria	664:701	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	3	58	theme	globulin	632:639	arg1	fragments					608:616	the extension region (ER) fragments	582:616	the extension region (ER) fragments of soybean 7S globulin	582:639	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	2	59	theme	pepsin-released	349:363	arg1	peptides					365:372	pepsin-released peptides	349:372	pepsin-released peptides of soybean 7S globulin on gut microbiota	349:413	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	3	60	dep	In	536:537	arg1	vitro					539:543	vitro	539:543	vitro	539:543	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	3	61	theme	Gram-negative	680:692	arg1	bacteria					694:701	proinflammatory Gram-negative bacteria	664:701	proinflammatory Gram-negative bacteria	664:701	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	6	62	theme	possible	1173:1180	arg1	explanation					1182:1192	a possible explanation	1171:1192	a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease	1171:1267	This finding supplies a possible explanation for improvement of the effects of soybean 7S globulin on metabolic disease.					
30719912	5	63	theme	high	1002:1005	arg1	affinity					1007:1014	high affinity	1002:1014	high affinity to LPS	1002:1021	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	5	64	theme	LPS	1133:1135	arg1	monolayers					1137:1146	LPS monolayers	1133:1146	LPS monolayers	1133:1146	Isothermal titration calorimetry (ITC) and Langmuir monolayer studies demonstrated that ER peptides exhibited high affinity to LPS in the presence of Ca2+ and developed into β-sheet-rich aggregate structures, thus weakening the stability of LPS monolayers.					
30719912	4	65	theme	Lachnospiraceae	854:868	arg1	abundance					841:849	the relative abundance	828:849	the relative abundance of Lachnospiraceae and Lactobacillaceae	828:889	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
30719912	0	66	theme	Gut	14:16	arg1	Microbiota					18:27	Gut Microbiota	14:27	Gut Microbiota	14:27	Modulation of Gut Microbiota by Soybean 7S Globulin Peptide That Involved Lipopolysaccharide-Peptide Interaction.					
30719912	2	67	theme	globulin	388:395	arg1	peptides					365:372	pepsin-released peptides	349:372	pepsin-released peptides of soybean 7S globulin on gut microbiota	349:413	This study aimed to investigate the modulation of pepsin-released peptides of soybean 7S globulin on gut microbiota and possible association between changes of gut microbiota composition and lipopolysaccharide (LPS)-peptide interaction.					
30719912	3	68	theme	In	536:537	arg1	experiments					558:568	In vitro fermentation experiments	536:568	In vitro fermentation experiments	536:568	In vitro fermentation experiments showed that the extension region (ER) fragments of soybean 7S globulin selectively suppressed proinflammatory Gram-negative bacteria.					
30719912	4	69	theme	acids	774:778	arg1	production					742:751	the highest production	730:751	the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae	730:889	ER peptides also promoted the highest production of short-chain fatty acids (SCFAs), which were associated with increase of the relative abundance of Lachnospiraceae and Lactobacillaceae.					
31132162	11	0	theme	PRACTICAL	1792:1800	arg1	by-products					1894:1904	aquatic by-products	1886:1904	aquatic by-products	1886:1904	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable.					
31132162	11	0	theme	PRACTICAL	1792:1800	arg1	APPLICATION					1802:1812	PRACTICAL APPLICATION	1792:1812	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study	1792:1880	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable.					
31132162	9	1	theme	bactericidal	1550:1561	arg1	104.12					1583:1588	104.12	1583:1588	104.12	1583:1588	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	9	1	theme	bactericidal	1550:1561	arg1	concentrations					1563:1576	the minimal bactericidal concentrations	1538:1576	the minimal bactericidal concentrations	1538:1576	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	10	2	theme	good	1668:1671	arg1	properties					1702:1711	good mechanical and antibacterial properties	1668:1711	good mechanical and antibacterial properties	1668:1711	These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising.					
31132162	5	3	theme	composite	806:814	arg1	film					816:819	the ternary composite film	794:819	the ternary composite film (CSGEo film, containing CS, GE, and CEo)	794:860	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	13	4	theme	excellent	2180:2188	arg1	properties					2201:2210	excellent mechanical properties	2180:2210	excellent mechanical properties	2180:2210	CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can, therefore, be used in the food packaging industry.					
31132162	3	5	theme	mechanical	520:529	arg1	properties					531:540	The mechanical properties	516:540	The mechanical properties	516:540	The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined.					
31132162	12	6	dep	crystallinity	2047:2059	arg1	the					2043:2045	the	2043:2045	the	2043:2045	The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials.					
31132162	12	7	theme	thermal	2010:2016	arg1	properties					2018:2027	the UV barrier and thermal properties	1991:2027	properties	2018:2027	The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials.					
31132162	12	8	theme	CEo	1978:1980	arg1	addition					1954:1961	The addition	1950:1961	The addition of gelatin and CEo	1950:1980	The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials.					
31132162	13	9	theme	antibacterial	2216:2228	arg1	activity					2230:2237	antibacterial activity	2216:2237	antibacterial activity	2216:2237	CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can, therefore, be used in the food packaging industry.					
31132162	0	10	theme	Films	72:76	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	0	10	theme	Films	72:76	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	11	11	theme	aquatic	1886:1892	arg1	APPLICATION					1802:1812	PRACTICAL APPLICATION	1792:1812	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study	1792:1880	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable.					
31132162	11	11	theme	aquatic	1886:1892	arg1	by-products					1894:1904	aquatic by-products	1886:1904	aquatic by-products	1886:1904	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable.					
31132162	1	12	theme	mechanical	166:175	arg1	properties					195:204	the mechanical and antibacterial properties	162:204	the mechanical and antibacterial properties of chitosan (CS) films	162:227	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	6	13	contain	had	960:962	arg2	surfaces					971:978	smooth surfaces	964:978	smooth surfaces	964:978	SEM images revealed that all films had smooth surfaces, although some obvious differences between CS and CSGEo films were observed by AFM.					
31132162	6	13	contain	had	960:962	arg1	films					954:958	all films	950:958	all films	950:958	SEM images revealed that all films had smooth surfaces, although some obvious differences between CS and CSGEo films were observed by AFM.					
31132162	12	14	theme	gelatin	1966:1972	arg1	addition					1954:1961	The addition	1950:1961	The addition of gelatin and CEo	1950:1980	The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials.					
31132162	0	15	theme	Efficient	83:91	arg1	Activities					107:116	Efficient Antimicrobial Activities	83:116	Efficient Antimicrobial Activities for Food Packaging Applications	83:148	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	1	16	theme	cinnamon	318:325	arg1	CEo					342:344	CEo	342:344	CEo	342:344	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	1	16	theme	cinnamon	318:325	arg1	oil					337:339	natural cinnamon essential oil	310:339	natural cinnamon essential oil (CEo)	310:345	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	1	17	theme	antibacterial	181:193	arg1	properties					195:204	the mechanical and antibacterial properties	162:204	the mechanical and antibacterial properties of chitosan (CS) films	162:227	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	4	18	theme	UV	719:720	arg1	protection					722:731	excellent UV protection	709:731	excellent UV protection	709:731	The results confirmed all of the films exhibited excellent UV protection with low transparency at 600 nm.					
31132162	5	19	theme	higher	868:873	arg1	elongation					875:884	a higher elongation	866:884	a higher elongation	866:884	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	2	20	theme	X-ray	460:464	arg1	diffraction					466:476	X-ray diffraction	460:476	X-ray diffraction	460:476	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	8	21	theme	activity	1244:1251	arg1	results					1253:1259	The antimicrobial activity results	1226:1259	The antimicrobial activity results	1226:1259	The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%.					
31132162	11	22	theme	raw	1824:1826	arg1	materials					1828:1836	The main raw materials	1815:1836	The main raw materials of the edible films	1815:1856	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable.					
31132162	0	23	theme	Food	122:125	arg1	Applications					137:148	Food Packaging Applications	122:148	Food Packaging Applications	122:148	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	5	24	theme	containing	834:843	arg1	CS					845:846	containing CS	834:846	containing CS	834:846	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	9	25	theme	CSGEo	1466:1470	arg1	solution					1472:1479	the CSGEo solution	1462:1479	the CSGEo solution	1462:1479	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	6	26	theme	smooth	964:969	arg1	surfaces					971:978	smooth surfaces	964:978	smooth surfaces	964:978	SEM images revealed that all films had smooth surfaces, although some obvious differences between CS and CSGEo films were observed by AFM.					
31132162	2	27	theme	Scanning	348:355	arg1	SEM					378:380	SEM	378:380	SEM	378:380	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	2	27	theme	Scanning	348:355	arg1	microscopy					366:375	Scanning electron microscopy	348:375	Scanning electron microscopy (SEM)	348:381	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	2	28	theme	force	391:395	arg1	microscopy					397:406	Atomic force microscopy	384:406	Atomic force microscopy (AFM)	384:412	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	2	28	theme	force	391:395	arg1	AFM					409:411	AFM	409:411	AFM	409:411	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	8	29	theme	CSGEo	1273:1277	arg1	films					1279:1283	CSGEo films	1273:1283	CSGEo films	1273:1283	The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%.					
31132162	12	30	theme	films	2087:2091	arg1	hydrophilicity					2065:2078	hydrophilicity	2065:2078	hydrophilicity	2065:2078	The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials.					
31132162	12	30	theme	films	2087:2091	arg1	crystallinity					2047:2059	crystallinity	2047:2059	crystallinity	2047:2059	The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials.					
31132162	11	31	theme	edible	1845:1850	arg1	films					1852:1856	the edible films	1841:1856	the edible films	1841:1856	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable.					
31132162	13	32	theme	CEo-incorporated	2147:2162	arg1	films					2164:2168	CEo-incorporated films	2147:2168	CEo-incorporated films	2147:2168	CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can, therefore, be used in the food packaging industry.					
31132162	3	33	theme	antibacterial	602:614	arg1	activity					616:623	antibacterial activity	602:623	antibacterial activity	602:623	The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined.					
31132162	5	34	dep	film	816:819	arg1	GE					849:850	GE	849:850	GE	849:850	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	5	34	dep	film	816:819	arg1	CEo					857:859	CEo	857:859	CEo	857:859	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	5	34	dep	film	816:819	arg1	CS					845:846	containing CS	834:846	containing CS	834:846	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	5	34	dep	film	816:819	arg1	film					828:831	CSGEo film	822:831	CSGEo film	822:831	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	0	35	theme	Chitosan-Based	36:49	arg1	Films					72:76	Chitosan-Based Ternary Blend Edible Films	36:76	Chitosan-Based Ternary Blend Edible Films	36:76	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	10	36	theme	packaging	1759:1767	arg1	industry					1769:1776	the food packaging industry	1750:1776	the food packaging industry	1750:1776	These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising.					
31132162	8	37	theme	antimicrobial	1299:1311	arg1	activity					1313:1320	excellent antimicrobial activity	1289:1320	excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%	1289:1418	The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%.					
31132162	0	38	with	Characterization	16:31	arg1	Activities					107:116	Efficient Antimicrobial Activities	83:116	Efficient Antimicrobial Activities for Food Packaging Applications	83:148	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	0	39	theme	Blend	59:63	arg1	Films					72:76	Chitosan-Based Ternary Blend Edible Films	36:76	Chitosan-Based Ternary Blend Edible Films	36:76	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	2	40	dep	transform	423:431	arg1	infrared					433:440	infrared	433:440	transform infrared spectroscopy	423:453	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	7	41	theme	contact	1160:1166	arg1	angle					1168:1172	contact angle	1160:1172	contact angle	1160:1172	Additionally, the incorporation of GE and CEo to the films enhanced their thermal stability and contact angle, but decreased their crystallinity and wettability.					
31132162	3	42	theme	thermal	563:569	arg1	stability					571:579	thermal stability	563:579	thermal stability	563:579	The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined.					
31132162	3	43	theme	films	632:636	arg1	properties					531:540	The mechanical properties	516:540	The mechanical properties	516:540	The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined.					
31132162	3	43	theme	films	632:636	arg1	transmission					549:560	light transmission	543:560	light transmission	543:560	The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined.					
31132162	3	43	theme	films	632:636	arg1	stability					571:579	thermal stability	563:579	thermal stability	563:579	The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined.					
31132162	3	43	theme	films	632:636	arg1	hydrophilicity					582:595	hydrophilicity	582:595	hydrophilicity	582:595	The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined.					
31132162	3	43	theme	films	632:636	arg1	activity					616:623	antibacterial activity	602:623	antibacterial activity	602:623	The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined.					
31132162	0	44	with	Preparation	0:10	arg1	Activities					107:116	Efficient Antimicrobial Activities	83:116	Efficient Antimicrobial Activities for Food Packaging Applications	83:148	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	13	45	used	used	2262:2265	arg2	films					2164:2168	CEo-incorporated films	2147:2168	CEo-incorporated films	2147:2168	CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can, therefore, be used in the food packaging industry.					
31132162	10	46	theme	CSGEo	1648:1652	arg1	films					1654:1658	CSGEo films	1648:1658	CSGEo films	1648:1658	These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising.					
31132162	1	47	theme	blend	240:244	arg1	film					253:256	a ternary blend edible film	230:256	a ternary blend edible film	230:256	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	5	48	theme	lower	901:905	arg1	strength					915:922	a lower tensile strength	899:922	a lower tensile strength	899:922	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	7	49	theme	GE	1099:1100	arg1	incorporation					1082:1094	the incorporation	1078:1094	the incorporation of GE and CEo to the films	1078:1121	Additionally, the incorporation of GE and CEo to the films enhanced their thermal stability and contact angle, but decreased their crystallinity and wettability.					
31132162	9	50	theme	minimal	1542:1548	arg1	104.12					1583:1588	104.12	1583:1588	104.12	1583:1588	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	9	50	theme	minimal	1542:1548	arg1	concentrations					1563:1576	the minimal bactericidal concentrations	1538:1576	the minimal bactericidal concentrations	1538:1576	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	10	51	theme	mechanical	1673:1682	arg1	properties					1702:1711	good mechanical and antibacterial properties	1668:1711	good mechanical and antibacterial properties	1668:1711	These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising.					
31132162	5	52	theme	ternary	798:804	arg1	film					816:819	the ternary composite film	794:819	the ternary composite film (CSGEo film, containing CS, GE, and CEo)	794:860	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	1	53	theme	chitosan	209:216	arg1	films					223:227	chitosan (CS) films	209:227	chitosan (CS) films	209:227	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	5	54	contain	had	862:864	arg2	elongation					875:884	a higher elongation	866:884	a higher elongation	866:884	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	5	54	contain	had	862:864	arg1	film					816:819	the ternary composite film	794:819	the ternary composite film (CSGEo film, containing CS, GE, and CEo)	794:860	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	10	55	theme	antibacterial	1688:1700	arg1	properties					1702:1711	good mechanical and antibacterial properties	1668:1711	good mechanical and antibacterial properties	1668:1711	These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising.					
31132162	5	56	theme	CS	784:785	arg1	films					787:791	the CS films	780:791	the CS films	780:791	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	6	57	theme	CSGEo	1030:1034	arg1	films					1036:1040	CS and CSGEo films	1023:1040	CS and CSGEo films	1023:1040	SEM images revealed that all films had smooth surfaces, although some obvious differences between CS and CSGEo films were observed by AFM.					
31132162	9	58	theme	inhibitory	1433:1442	arg1	52.06 µg/mL					1521:1531	both 52.06 µg/mL	1516:1531	both 52.06 µg/mL	1516:1531	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	9	58	theme	inhibitory	1433:1442	arg1	concentrations					1444:1457	The minimum inhibitory concentrations	1421:1457	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus	1421:1509	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	12	59	theme	UV	1995:1996	arg1	barrier					1998:2004	the UV barrier and thermal properties	1991:2027	barrier	1998:2004	The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials.					
31132162	6	60	theme	CS	1023:1024	arg1	films					1036:1040	CS and CSGEo films	1023:1040	CS and CSGEo films	1023:1040	SEM images revealed that all films had smooth surfaces, although some obvious differences between CS and CSGEo films were observed by AFM.					
31132162	3	61	theme	light	543:547	arg1	transmission					549:560	light transmission	543:560	light transmission	543:560	The mechanical properties, light transmission, thermal stability, hydrophilicity, and antibacterial activity of the films were also determined.					
31132162	1	62	theme	films	223:227	arg1	properties					195:204	the mechanical and antibacterial properties	162:204	the mechanical and antibacterial properties of chitosan (CS) films	162:227	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	13	63	theme	mechanical	2190:2199	arg1	properties					2201:2210	excellent mechanical properties	2180:2210	excellent mechanical properties	2180:2210	CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can, therefore, be used in the food packaging industry.					
31132162	10	64	theme	food	1754:1757	arg1	industry					1769:1776	the food packaging industry	1750:1776	the food packaging industry	1750:1776	These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising.					
31132162	6	65	theme	SEM	925:927	arg1	images					929:934	SEM images	925:934	SEM images	925:934	SEM images revealed that all films had smooth surfaces, although some obvious differences between CS and CSGEo films were observed by AFM.					
31132162	8	66	theme	antibacterial	1388:1400	arg1	rate					1402:1405	the antibacterial rate	1384:1405	the antibacterial rate	1384:1405	The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%.					
31132162	12	67	theme	packaging	2126:2134	arg1	materials					2136:2144	packaging materials	2126:2144	packaging materials	2126:2144	The addition of gelatin and CEo improved the UV barrier and thermal properties but decreased the crystallinity and hydrophilicity of the films, making them suitable for use as packaging materials.					
31132162	7	68	theme	CEo	1106:1108	arg1	incorporation					1082:1094	the incorporation	1078:1094	the incorporation of GE and CEo to the films	1078:1121	Additionally, the incorporation of GE and CEo to the films enhanced their thermal stability and contact angle, but decreased their crystallinity and wettability.					
31132162	5	69	theme	CSGEo	822:826	arg1	film					828:831	CSGEo film	822:831	CSGEo film	822:831	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	10	70	contain	possess	1660:1666	arg1	films					1654:1658	CSGEo films	1648:1658	CSGEo films	1648:1658	These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising.					
31132162	10	70	contain	possess	1660:1666	arg2	properties					1702:1711	good mechanical and antibacterial properties	1668:1711	good mechanical and antibacterial properties	1668:1711	These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising.					
31132162	0	71	theme	Antimicrobial	93:105	arg1	Activities					107:116	Efficient Antimicrobial Activities	83:116	Efficient Antimicrobial Activities for Food Packaging Applications	83:148	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	1	72	theme	natural	310:316	arg1	CEo					342:344	CEo	342:344	CEo	342:344	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	1	72	theme	natural	310:316	arg1	oil					337:339	natural cinnamon essential oil	310:339	natural cinnamon essential oil (CEo)	310:345	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	9	73	theme	minimum	1425:1431	arg1	52.06 µg/mL					1521:1531	both 52.06 µg/mL	1516:1531	both 52.06 µg/mL	1516:1531	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	9	73	theme	minimum	1425:1431	arg1	concentrations					1444:1457	The minimum inhibitory concentrations	1421:1457	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus	1421:1509	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	1	74	theme	essential	327:335	arg1	CEo					342:344	CEo	342:344	CEo	342:344	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	1	74	theme	essential	327:335	arg1	oil					337:339	natural cinnamon essential oil	310:339	natural cinnamon essential oil (CEo)	310:345	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	2	75	dep	Fourier	415:421	arg1	transform					423:431	transform	423:431	transform infrared spectroscopy	423:453	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	4	76	theme	excellent	709:717	arg1	protection					722:731	excellent UV protection	709:731	excellent UV protection	709:731	The results confirmed all of the films exhibited excellent UV protection with low transparency at 600 nm.					
31132162	0	77	theme	Packaging	127:135	arg1	Applications					137:148	Food Packaging Applications	122:148	Food Packaging Applications	122:148	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	8	78	theme	antimicrobial	1230:1242	arg1	results					1253:1259	The antimicrobial activity results	1226:1259	The antimicrobial activity results	1226:1259	The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%.					
31132162	2	79	theme	electron	357:364	arg1	SEM					378:380	SEM	378:380	SEM	378:380	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	2	79	theme	electron	357:364	arg1	microscopy					366:375	Scanning electron microscopy	348:375	Scanning electron microscopy (SEM)	348:381	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	9	80	theme	solution	1472:1479	arg1	52.06 µg/mL					1521:1531	both 52.06 µg/mL	1516:1531	both 52.06 µg/mL	1516:1531	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	9	80	theme	solution	1472:1479	arg1	concentrations					1444:1457	The minimum inhibitory concentrations	1421:1457	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus	1421:1509	The minimum inhibitory concentrations of the CSGEo solution against E. coli and S. aureus were both 52.06 µg/mL, and the minimal bactericidal concentrations were 104.12 and 52.06 µg/mL, respectively.					
31132162	11	81	theme	main	1819:1822	arg1	materials					1828:1836	The main raw materials	1815:1836	The main raw materials of the edible films	1815:1856	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable.					
31132162	11	82	dep	APPLICATION	1802:1812	arg1	developed					1858:1866	developed	1858:1866	developed in this study	1858:1880	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable.					
31132162	10	83	from	application	1735:1745	arg1	industry					1769:1776	the food packaging industry	1750:1776	the food packaging industry	1750:1776	These results suggest that CSGEo films possess good mechanical and antibacterial properties, and therefore, their application in the food packaging industry is promising.					
31132162	2	84	theme	Atomic	384:389	arg1	microscopy					397:406	Atomic force microscopy	384:406	Atomic force microscopy (AFM)	384:412	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	2	84	theme	Atomic	384:389	arg1	AFM					409:411	AFM	409:411	AFM	409:411	Scanning electron microscopy (SEM), Atomic force microscopy (AFM), Fourier transform infrared spectroscopy, and X-ray diffraction were performed to evaluate the films.					
31132162	11	85	theme	films	1852:1856	arg1	materials					1828:1836	The main raw materials	1815:1836	The main raw materials of the edible films	1815:1856	PRACTICAL APPLICATION: The main raw materials of the edible films developed in this study are aquatic by-products, so the films are edible and biodegradable.					
31132162	0	86	theme	Ternary	51:57	arg1	Films					72:76	Chitosan-Based Ternary Blend Edible Films	36:76	Chitosan-Based Ternary Blend Edible Films	36:76	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	5	87	theme	tensile	907:913	arg1	strength					915:922	a lower tensile strength	899:922	a lower tensile strength	899:922	Compared with the CS films, the ternary composite film (CSGEo film, containing CS, GE, and CEo) had a higher elongation at break but a lower tensile strength.					
31132162	0	88	theme	Edible	65:70	arg1	Films					72:76	Chitosan-Based Ternary Blend Edible Films	36:76	Chitosan-Based Ternary Blend Edible Films	36:76	Preparation and Characterization of Chitosan-Based Ternary Blend Edible Films with Efficient Antimicrobial Activities for Food Packaging Applications.					
31132162	8	89	contain	had	1285:1287	arg1	films					1279:1283	CSGEo films	1273:1283	CSGEo films	1273:1283	The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%.					
31132162	8	89	contain	had	1285:1287	arg2	activity					1313:1320	excellent antimicrobial activity	1289:1320	excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%	1289:1418	The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%.					
31132162	8	90	theme	excellent	1289:1297	arg1	activity					1313:1320	excellent antimicrobial activity	1289:1320	excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%	1289:1418	The antimicrobial activity results showed that CSGEo films had excellent antimicrobial activity against Escherichia coli and Staphylococcus aureus, for which the antibacterial rate exceeded 98%.					
31132162	13	91	theme	packaging	2279:2287	arg1	industry					2289:2296	the food packaging industry	2270:2296	the food packaging industry	2270:2296	CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can, therefore, be used in the food packaging industry.					
31132162	7	92	theme	thermal	1138:1144	arg1	stability					1146:1154	their thermal stability	1132:1154	their thermal stability	1132:1154	Additionally, the incorporation of GE and CEo to the films enhanced their thermal stability and contact angle, but decreased their crystallinity and wettability.					
31132162	4	93	theme	low	738:740	arg1	transparency					742:753	low transparency	738:753	low transparency	738:753	The results confirmed all of the films exhibited excellent UV protection with low transparency at 600 nm.					
31132162	1	94	theme	ternary	232:238	arg1	film					253:256	a ternary blend edible film	230:256	a ternary blend edible film	230:256	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31132162	13	95	theme	food	2274:2277	arg1	industry					2289:2296	the food packaging industry	2270:2296	the food packaging industry	2270:2296	CEo-incorporated films exhibited excellent mechanical properties and antibacterial activity and can, therefore, be used in the food packaging industry.					
31132162	6	96	theme	obvious	995:1001	arg1	differences					1003:1013	some obvious differences	990:1013	some obvious differences between CS and CSGEo films	990:1040	SEM images revealed that all films had smooth surfaces, although some obvious differences between CS and CSGEo films were observed by AFM.					
31132162	1	97	theme	edible	246:251	arg1	film					253:256	a ternary blend edible film	230:256	a ternary blend edible film	230:256	To improve the mechanical and antibacterial properties of chitosan (CS) films, a ternary blend edible film was prepared by incorporating CS, gelatin (GE), and natural cinnamon essential oil (CEo).					
31094142	8	0	theme	citrate	1165:1171	arg1	nanoparticles					1122:1134	nanoparticles	1122:1134	nanoparticles of hydroxyapatite or calcium citrate	1122:1171	Addition of nanoparticles of hydroxyapatite or calcium citrate to dentifrices improved their adherence to dentin without any pre-treatment.					
31094142	10	1	theme	pastes	1671:1676	arg1	ability					1648:1654	The ability	1644:1654	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules	1644:1756	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	5	2	theme	calcium-based	827:839	arg1	nanoparticles					841:853	calcium-based nanoparticles	827:853	calcium-based nanoparticles	827:853	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	10	3	theme	desensitizing	1785:1797	arg1	dentifrices					1799:1809	desensitizing dentifrices	1785:1809	desensitizing dentifrices	1785:1809	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	7	4	theme	calcium	1046:1052	arg1	nanoparticles					1064:1076	calcium carbonate nanoparticles	1046:1076	calcium carbonate nanoparticles	1046:1076	Pretreatment by L-arginine improved adherence of calcium carbonate nanoparticles, but less efficiently than ChS.					
31094142	4	5	theme	elemental	577:585	arg1	composition					587:597	the elemental composition	573:597	the elemental composition of the deposits	573:613	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	0	6	theme	tubule	80:85	arg1	occlusion					87:95	dentin tubule occlusion	73:95	dentin tubule occlusion	73:95	Pre-treatment of dentin with chondroitin sulfate or L-arginine modulates dentin tubule occlusion by toothpaste components.					
31094142	4	7	theme	dentin	507:512	arg1	tubules					514:520	dentin tubules	507:520	dentin tubules	507:520	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	9	8	theme	two-step	1503:1510	arg1	treatments					1519:1528	two-step dental treatments	1503:1528	two-step dental treatments	1503:1528	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	0	9	theme	toothpaste	100:109	arg1	components					111:120	toothpaste components	100:120	toothpaste components	100:120	Pre-treatment of dentin with chondroitin sulfate or L-arginine modulates dentin tubule occlusion by toothpaste components.					
31094142	9	10	theme	dentifrices	1335:1345	arg1	surface					1324:1330	the dentin surface	1313:1330	the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles	1313:1395	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	5	11	theme	Rinsing	678:684	arg1	sample					695:700	Rinsing a dentin sample	678:700	Rinsing a dentin sample with a solution of ChS	678:723	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	9	12	with	pre-treatment	1429:1441	arg1	L-arginine					1455:1464	L-arginine	1455:1464	L-arginine	1455:1464	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	9	12	with	pre-treatment	1429:1441	arg1	ChS					1448:1450	ChS	1448:1450	ChS	1448:1450	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	9	13	theme	active	1592:1597	arg1	components					1610:1619	active dentifrice components	1592:1619	active dentifrice components	1592:1619	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	10	14	theme	calcium	1716:1722	arg1	citrate					1724:1730	calcium citrate	1716:1730	calcium citrate	1716:1730	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	3	15	theme	L-arginine	350:359	arg1	solution					308:315	solution	308:315	solution of chondroitin sulfate ( ChS) or L-arginine prior to application of a commercial or custom-made toothpaste.	308:423	solution of chondroitin sulfate ( ChS) or L-arginine prior to application of a commercial or custom-made toothpaste.					
31094142	10	16	theme	tooth	1871:1875	arg1	surface					1877:1883	the tooth surface	1867:1883	the tooth surface	1867:1883	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	5	17	theme	a	686:686	arg1	sample					695:700	Rinsing a dentin sample	678:700	Rinsing a dentin sample with a solution of ChS	678:723	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	1	18	from	effect	147:152	arg1	dentin					157:162	dentin	157:162	dentin of chondroitin sulfate and L-arginine	157:200	PURPOSE To evaluate the effect on dentin of chondroitin sulfate and L-arginine on dentin tubule occlusion.					
31094142	1	18	from	effect	147:152	arg1	occlusion					219:227	dentin tubule occlusion	205:227	dentin tubule occlusion	205:227	PURPOSE To evaluate the effect on dentin of chondroitin sulfate and L-arginine on dentin tubule occlusion.					
31094142	9	19	theme	dentifrice	1599:1608	arg1	components					1610:1619	active dentifrice components	1592:1619	active dentifrice components	1592:1619	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	6	20	theme	dentifrices	971:981	arg1	adherence					958:966	the adherence	954:966	the adherence of dentifrices lacking TiO₂	954:994	ChS does not appear to enhance the adherence of dentifrices lacking TiO₂.					
31094142	5	21	theme	dentifrices	769:779	arg1	adherence					756:764	the adherence	752:764	the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface	752:920	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	9	22	theme	treatments	1519:1528	arg1	development					1488:1498	the development	1484:1498	the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface	1484:1641	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	10	23	theme	hydroxyapatite	1698:1711	arg1	nanoparticles					1681:1693	nanoparticles	1681:1693	nanoparticles of hydroxyapatite or calcium citrate	1681:1730	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	1	24	theme	chondroitin	167:177	arg1	sulfate					179:185	chondroitin sulfate	167:185	chondroitin sulfate	167:185	PURPOSE To evaluate the effect on dentin of chondroitin sulfate and L-arginine on dentin tubule occlusion.					
31094142	9	25	theme	dental	1512:1517	arg1	treatments					1519:1528	two-step dental treatments	1503:1528	two-step dental treatments	1503:1528	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	9	26	theme	TiO₂	1357:1360	arg1	dentifrices					1335:1345	dentifrices	1335:1345	dentifrices of either TiO₂ or calcium-supplying nanoparticles	1335:1395	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	9	27	theme	significant	1276:1286	arg1	increase					1288:1295	The significant increase	1272:1295	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine	1250:1464	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	1	28	theme	sulfate	179:185	arg1	dentin					157:162	dentin	157:162	dentin of chondroitin sulfate and L-arginine	157:200	PURPOSE To evaluate the effect on dentin of chondroitin sulfate and L-arginine on dentin tubule occlusion.					
31094142	3	29	theme	chondroitin	320:330	arg1	sulfate					332:338	chondroitin sulfate	320:338	chondroitin sulfate ( ChS)	320:345	solution of chondroitin sulfate ( ChS) or L-arginine prior to application of a commercial or custom-made toothpaste.					
31094142	3	29	theme	chondroitin	320:330	arg1	ChS					342:344	ChS	342:344	ChS	342:344	solution of chondroitin sulfate ( ChS) or L-arginine prior to application of a commercial or custom-made toothpaste.					
31094142	3	30	theme	sulfate	332:338	arg1	solution					308:315	solution	308:315	solution of chondroitin sulfate ( ChS) or L-arginine prior to application of a commercial or custom-made toothpaste.	308:423	solution of chondroitin sulfate ( ChS) or L-arginine prior to application of a commercial or custom-made toothpaste.					
31094142	3	31	theme	toothpaste	413:422	arg1	application					370:380	application	370:380	application of a commercial or custom-made toothpaste	370:422	solution of chondroitin sulfate ( ChS) or L-arginine prior to application of a commercial or custom-made toothpaste.					
31094142	7	32	theme	nanoparticles	1064:1076	arg1	adherence					1033:1041	adherence	1033:1041	adherence of calcium carbonate nanoparticles	1033:1076	Pretreatment by L-arginine improved adherence of calcium carbonate nanoparticles, but less efficiently than ChS.					
31094142	4	33	theme	electron	549:556	arg1	microscopy					558:567	scanning electron microscopy	540:567	scanning electron microscopy	540:567	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	9	34	theme	dentin	1628:1633	arg1	surface					1635:1641	the dentin surface	1624:1641	the dentin surface	1624:1641	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	2	35	dep	activated	262:270	arg1	aq.					302:304	aq.	302:304	aq.	302:304	METHODS The dentin samples were activated by submersion in an aqueous ( aq. )					
31094142	4	36	theme	scanning	540:547	arg1	microscopy					558:567	scanning electron microscopy	540:567	scanning electron microscopy	540:567	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	10	37	theme	dentin	1743:1748	arg1	tubules					1750:1756	dentin tubules	1743:1756	dentin tubules	1743:1756	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	9	38	theme	CLINICAL	1250:1257	arg1	SIGNIFICANCE					1259:1270	CLINICAL SIGNIFICANCE	1250:1270	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine	1250:1464	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	2	39	dep	METHODS	230:236	arg1	activated					262:270	activated	262:270	were activated by submersion in an aqueous ( aq. )	257:306	METHODS The dentin samples were activated by submersion in an aqueous ( aq. )					
31094142	0	40	theme	dentin	17:22	arg1	Pre-treatment					0:12	Pre-treatment	0:12	Pre-treatment of dentin with chondroitin sulfate or L-arginine	0:61	Pre-treatment of dentin with chondroitin sulfate or L-arginine modulates dentin tubule occlusion by toothpaste components.					
31094142	9	41	theme	calcium-supplying	1365:1381	arg1	nanoparticles					1383:1395	calcium-supplying nanoparticles	1365:1395	calcium-supplying nanoparticles	1365:1395	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	5	42	from	increase	740:747	arg1	adherence					756:764	the adherence	752:764	the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface	752:920	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	10	43	theme	mineral	1834:1840	arg1	nutrients					1854:1862	the mineral and organic nutrients	1830:1862	the mineral and organic nutrients to the tooth surface	1830:1883	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	1	44	theme	dentin	205:210	arg1	occlusion					219:227	dentin tubule occlusion	205:227	dentin tubule occlusion	205:227	PURPOSE To evaluate the effect on dentin of chondroitin sulfate and L-arginine on dentin tubule occlusion.					
31094142	10	45	theme	nanoparticles	1681:1693	arg1	pastes					1671:1676	the aqueous pastes	1659:1676	the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate	1659:1730	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	5	46	theme	ChS	721:723	arg1	solution					709:716	a solution	707:716	a solution of ChS	707:723	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	2	47	theme	dentin	242:247	arg1	samples					249:255	The dentin samples	238:255	The dentin samples	238:255	METHODS The dentin samples were activated by submersion in an aqueous ( aq. )					
31094142	1	48	theme	L-arginine	191:200	arg1	dentin					157:162	dentin	157:162	dentin of chondroitin sulfate and L-arginine	157:200	PURPOSE To evaluate the effect on dentin of chondroitin sulfate and L-arginine on dentin tubule occlusion.					
31094142	1	49	theme	tubule	212:217	arg1	occlusion					219:227	dentin tubule occlusion	205:227	dentin tubule occlusion	205:227	PURPOSE To evaluate the effect on dentin of chondroitin sulfate and L-arginine on dentin tubule occlusion.					
31094142	0	50	theme	chondroitin	29:39	arg1	sulfate					41:47	chondroitin sulfate	29:47	chondroitin sulfate	29:47	Pre-treatment of dentin with chondroitin sulfate or L-arginine modulates dentin tubule occlusion by toothpaste components.					
31094142	9	51	theme	dentin	1404:1409	arg1	surface					1411:1417	the dentin surface	1400:1417	the dentin surface following pre-treatment with ChS or L-arginine	1400:1464	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	3	52	theme	custom-made	401:411	arg1	toothpaste					413:422	a commercial or custom-made toothpaste	385:422	a commercial or custom-made toothpaste	385:422	solution of chondroitin sulfate ( ChS) or L-arginine prior to application of a commercial or custom-made toothpaste.					
31094142	8	53	theme	calcium	1157:1163	arg1	citrate					1165:1171	calcium citrate	1157:1171	calcium citrate	1157:1171	Addition of nanoparticles of hydroxyapatite or calcium citrate to dentifrices improved their adherence to dentin without any pre-treatment.					
31094142	9	54	theme	nanoparticles	1383:1395	arg1	dentifrices					1335:1345	dentifrices	1335:1345	dentifrices of either TiO₂ or calcium-supplying nanoparticles	1335:1395	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	5	55	theme	hydroxyapatite	857:870	arg1	HA\					874:876	hydroxyapatite ( HA\ or calcium carbonate	857:897	HA\	874:876	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	4	56	theme	x-ray	650:654	arg1	spectroscopy					656:667	energy-dispersive x-ray spectroscopy	632:667	energy-dispersive x-ray spectroscopy	632:667	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	4	57	with	rinsing	431:437	arg1	water					444:448	water	444:448	water	444:448	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	4	57	with	rinsing	431:437	arg1	ultrasonication					454:468	ultrasonication	454:468	ultrasonication	454:468	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	4	58	theme	tubules	514:520	arg1	adhesion					471:478	adhesion	471:478	adhesion to dentin and occlusion of dentin tubules	471:520	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	5	59	theme	dentin	688:693	arg1	sample					695:700	Rinsing a dentin sample	678:700	Rinsing a dentin sample with a solution of ChS	678:723	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	8	60	theme	hydroxyapatite	1139:1152	arg1	nanoparticles					1122:1134	nanoparticles	1122:1134	nanoparticles of hydroxyapatite or calcium citrate	1122:1171	Addition of nanoparticles of hydroxyapatite or calcium citrate to dentifrices improved their adherence to dentin without any pre-treatment.					
31094142	5	61	theme	dentin	907:912	arg1	surface					914:920	the dentin surface	903:920	the dentin surface	903:920	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	3	62	theme	commercial	387:396	arg1	toothpaste					413:422	a commercial or custom-made toothpaste	385:422	a commercial or custom-made toothpaste	385:422	solution of chondroitin sulfate ( ChS) or L-arginine prior to application of a commercial or custom-made toothpaste.					
31094142	7	63	theme	carbonate	1054:1062	arg1	nanoparticles					1064:1076	calcium carbonate nanoparticles	1046:1076	calcium carbonate nanoparticles	1046:1076	Pretreatment by L-arginine improved adherence of calcium carbonate nanoparticles, but less efficiently than ChS.					
31094142	9	64	theme	dentin	1548:1553	arg1	occlusion					1562:1570	dentin tubule occlusion	1548:1570	dentin tubule occlusion	1548:1570	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	5	65	dep	adherence	756:764	arg1	HA\					874:876	hydroxyapatite ( HA\ or calcium carbonate	857:897	HA\	874:876	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	5	65	dep	adherence	756:764	arg1	carbonate					889:897	hydroxyapatite ( HA\ or calcium carbonate	857:897	carbonate	889:897	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	0	66	with	Pre-treatment	0:12	arg1	sulfate					41:47	chondroitin sulfate	29:47	chondroitin sulfate	29:47	Pre-treatment of dentin with chondroitin sulfate or L-arginine modulates dentin tubule occlusion by toothpaste components.					
31094142	0	66	with	Pre-treatment	0:12	arg1	L-arginine					52:61	L-arginine	52:61	L-arginine	52:61	Pre-treatment of dentin with chondroitin sulfate or L-arginine modulates dentin tubule occlusion by toothpaste components.					
31094142	9	67	theme	tubule	1555:1560	arg1	occlusion					1562:1570	dentin tubule occlusion	1548:1570	dentin tubule occlusion	1548:1570	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	5	68	theme	calcium	881:887	arg1	carbonate					889:897	hydroxyapatite ( HA\ or calcium carbonate	857:897	carbonate	889:897	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	4	69	theme	deposits	606:613	arg1	composition					587:597	the elemental composition	573:597	the elemental composition of the deposits	573:613	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	8	70	theme	nanoparticles	1122:1134	arg1	Addition					1110:1117	Addition	1110:1117	Addition of nanoparticles of hydroxyapatite or calcium citrate to dentifrices	1110:1186	Addition of nanoparticles of hydroxyapatite or calcium citrate to dentifrices improved their adherence to dentin without any pre-treatment.					
31094142	10	71	theme	citrate	1724:1730	arg1	nanoparticles					1681:1693	nanoparticles	1681:1693	nanoparticles of hydroxyapatite or calcium citrate	1681:1730	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	5	72	with	sample	695:700	arg1	solution					709:716	a solution	707:716	a solution of ChS	707:723	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	0	73	theme	dentin	73:78	arg1	occlusion					87:95	dentin tubule occlusion	73:95	dentin tubule occlusion	73:95	Pre-treatment of dentin with chondroitin sulfate or L-arginine modulates dentin tubule occlusion by toothpaste components.					
31094142	5	74	contain	containing	781:790	arg2	TiO₂					817:820	TiO₂	817:820	TiO₂	817:820	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	5	74	contain	containing	781:790	arg2	dioxide					808:814	titanium dioxide	799:814	titanium dioxide (TiO₂ )	799:822	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	5	74	contain	containing	781:790	arg2	nanoparticles					841:853	calcium-based nanoparticles	827:853	calcium-based nanoparticles	827:853	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	5	74	contain	containing	781:790	arg1	dentifrices					769:779	dentifrices	769:779	dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles	769:853	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	10	75	theme	aqueous	1663:1669	arg1	pastes					1671:1676	the aqueous pastes	1659:1676	the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate	1659:1730	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	4	76	theme	energy-dispersive	632:648	arg1	spectroscopy					656:667	energy-dispersive x-ray spectroscopy	632:667	energy-dispersive x-ray spectroscopy	632:667	After rinsing with water and ultrasonication, adhesion to dentin and occlusion of dentin tubules were evaluated by scanning electron microscopy and the elemental composition of the deposits was evaluated by energy-dispersive x-ray spectroscopy.					
31094142	9	77	dep	SIGNIFICANCE	1259:1270	arg1	increase					1288:1295	The significant increase	1272:1295	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine	1250:1464	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	5	78	theme	titanium	799:806	arg1	TiO₂					817:820	TiO₂	817:820	TiO₂	817:820	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	5	78	theme	titanium	799:806	arg1	dioxide					808:814	titanium dioxide	799:814	titanium dioxide (TiO₂ )	799:822	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	9	79	theme	dentin	1317:1322	arg1	surface					1324:1330	the dentin surface	1313:1330	the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles	1313:1395	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
31094142	5	80	dep	RESULTS	670:676	arg1	resulted					725:732	resulted	725:732	resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface	725:920	RESULTS Rinsing a dentin sample with a solution of ChS resulted in an increase in the adherence of dentifrices containing either titanium dioxide (TiO₂ ) or calcium-based nanoparticles [ hydroxyapatite ( HA\ or calcium carbonate( to the dentin surface.					
31094142	10	81	theme	dentifrices	1799:1809	arg1	formulation					1770:1780	the formulation	1766:1780	the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface	1766:1883	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	10	82	theme	organic	1846:1852	arg1	nutrients					1854:1862	the mineral and organic nutrients	1830:1862	the mineral and organic nutrients to the tooth surface	1830:1883	The ability of the aqueous pastes of nanoparticles of hydroxyapatite or calcium citrate to occlude dentin tubules enables the formulation of desensitizing dentifrices, which also supply the mineral and organic nutrients to the tooth surface.					
31094142	9	83	from	increase	1288:1295	arg1	adherence					1300:1308	adherence	1300:1308	adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine	1300:1464	CLINICAL SIGNIFICANCE The significant increase in adherence to the dentin surface of dentifrices of either TiO₂ or calcium-supplying nanoparticles to the dentin surface following pre-treatment with ChS or L-arginine opens the door to the development of two-step dental treatments, which accomplish dentin tubule occlusion and help to deliver active dentifrice components to the dentin surface.					
30500765	3	0	from	carbohydrate	659:670	arg1	material					697:704	pretreated material	686:704	pretreated material	686:704	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	1	1	theme	material	334:341	arg1	digestibility					389:401	enzymatic digestibility	379:401	enzymatic digestibility of glucan in SMS	379:418	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	1	theme	material	334:341	arg1	composition					303:313	chemical composition	294:313	chemical composition of heat-pretreated material	294:341	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	1	theme	material	334:341	arg1	substrate					362:370	spent mushroom substrate	347:370	spent mushroom substrate	347:370	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	1	theme	material	334:341	arg1	growth					276:281	shiitake growth and yield	267:291	growth	276:281	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	1	theme	material	334:341	arg1	SMS					373:375	SMS	373:375	SMS	373:375	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	1	theme	material	334:341	arg1	yield					287:291	shiitake growth and yield	267:291	yield	287:291	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	1	theme	material	334:341	arg1	yield					448:452	theoretical bioethanol yield	425:452	theoretical bioethanol yield	425:452	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	2	from	composition	303:313	arg1	SMS					416:418	SMS	416:418	SMS	416:418	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	4	3	theme	initial	866:872	arg1	half					854:857	less than half	844:857	less than half of the initial	844:872	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	3	4	theme	lignin	676:681	arg1	SMS					710:712	SMS	710:712	SMS	710:712	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	3	4	theme	lignin	676:681	arg1	fractions					646:654	the fractions	642:654	the fractions of carbohydrate and lignin in pretreated material	642:704	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	4	5	theme	less	844:847	arg1	half					854:857	less than half	844:857	less than half of the initial	844:872	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	4	6	theme	than	849:852	arg1	half					854:857	less than half	844:857	less than half of the initial	844:872	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	4	7	theme	mass	913:916	arg1	fraction					918:925	the mass fraction	909:925	the mass fraction of glucan	909:935	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	1	8	theme	spent	347:351	arg1	substrate					362:370	spent mushroom substrate	347:370	spent mushroom substrate	347:370	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	9	from	digestibility	389:401	arg1	SMS					416:418	SMS	416:418	SMS	416:418	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	10	theme	mushroom	353:360	arg1	substrate					362:370	spent mushroom substrate	347:370	spent mushroom substrate	347:370	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	2	11	theme	fruit-body	569:578	arg1	yield					580:584	higher or comparable fruit-body yield	548:584	higher or comparable fruit-body yield	548:584	Compared to autoclaving, HAP resulted in faster mycelial growth, earlier fructification, and higher or comparable fruit-body yield.					
30500765	1	12	theme	theoretical	425:435	arg1	yield					448:452	theoretical bioethanol yield	425:452	theoretical bioethanol yield	425:452	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	2	13	theme	faster	496:501	arg1	growth					512:517	faster mycelial growth	496:517	faster mycelial growth	496:517	Compared to autoclaving, HAP resulted in faster mycelial growth, earlier fructification, and higher or comparable fruit-body yield.					
30500765	1	14	theme	bioethanol	437:446	arg1	yield					448:452	theoretical bioethanol yield	425:452	theoretical bioethanol yield	425:452	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	6	15	theme	bioethanol	1149:1158	arg1	production					1160:1169	bioethanol production	1149:1169	bioethanol production	1149:1169	Thus, HAP could substitute autoclaving and facilitate combined shiitake mushroom and bioethanol production.					
30500765	4	16	theme	glucan	971:976	arg1	hydrolysis					957:966	enzymatic hydrolysis	947:966	enzymatic hydrolysis of glucan	947:976	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	1	17	from	yield	287:291	arg1	SMS					416:418	SMS	416:418	SMS	416:418	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	3	18	from	SMS	710:712	arg1	material					697:704	pretreated material	686:704	pretreated material	686:704	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	1	19	theme	birch-wood-based	166:181	arg1	substrate					183:191	birch-wood-based substrate	166:191	birch-wood-based substrate	166:191	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	0	20	theme	substrate	17:25	arg1	pasteurisation					27:40	Energy-efficient substrate pasteurisation	0:40	Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol.	0:120	Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol.					
30500765	1	21	theme	substrate	183:191	arg1	HAP					158:160	HAP	158:160	HAP	158:160	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	21	theme	substrate	183:191	arg1	pasteurisation					142:155	Hot-air (75-100 °C) pasteurisation	122:155	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate	122:191	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	0	22	theme	Energy-efficient	0:15	arg1	pasteurisation					27:40	Energy-efficient substrate pasteurisation	0:40	Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol.	0:120	Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol.					
30500765	1	23	theme	shiitake	267:274	arg1	growth					276:281	shiitake growth and yield	267:291	growth	276:281	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	23	theme	shiitake	267:274	arg1	SMS					373:375	SMS	373:375	SMS	373:375	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	24	from	growth	276:281	arg1	SMS					416:418	SMS	416:418	SMS	416:418	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	6	25	theme	shiitake	1127:1134	arg1	mushroom					1136:1143	combined shiitake mushroom	1118:1143	combined shiitake mushroom	1118:1143	Thus, HAP could substitute autoclaving and facilitate combined shiitake mushroom and bioethanol production.					
30500765	3	26	theme	extractives	764:774	arg1	fractions					751:759	lower fractions	745:759	lower fractions of extractives	745:774	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	4	27	theme	major	893:897	arg1	impact					899:904	any major impact	889:904	any major impact on the mass fraction of glucan	889:935	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	6	28	theme	combined	1118:1125	arg1	mushroom					1136:1143	combined shiitake mushroom	1118:1143	combined shiitake mushroom	1118:1143	Thus, HAP could substitute autoclaving and facilitate combined shiitake mushroom and bioethanol production.					
30500765	4	29	theme	enzymatic	947:955	arg1	hydrolysis					957:966	enzymatic hydrolysis	947:966	enzymatic hydrolysis of glucan	947:976	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	1	30	from	substrate	362:370	arg1	SMS					416:418	SMS	416:418	SMS	416:418	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	3	31	dep	pretreatment	596:607	arg1	methods					609:615	methods	609:615	The heat pretreatment methods	587:615	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	3	32	from	lignin	676:681	arg1	material					697:704	pretreated material	686:704	pretreated material	686:704	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	2	33	theme	earlier	520:526	arg1	fructification					528:541	earlier fructification	520:541	earlier fructification	520:541	Compared to autoclaving, HAP resulted in faster mycelial growth, earlier fructification, and higher or comparable fruit-body yield.					
30500765	4	34	from	impact	899:904	arg1	fraction					918:925	the mass fraction	909:925	the mass fraction of glucan	909:935	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	1	35	theme	conventional	209:220	arg1	autoclaving					222:232	conventional autoclaving	209:232	conventional autoclaving (steam at 121 °C)	209:250	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	0	36	theme	combined	46:53	arg1	production					55:64	combined production	46:64	combined production of shiitake mushroom (Lentinula edodes)	46:104	Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol.					
30500765	1	37	theme	enzymatic	379:387	arg1	digestibility					389:401	enzymatic digestibility	379:401	enzymatic digestibility of glucan in SMS	379:418	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	0	38	theme	shiitake	69:76	arg1	mushroom					78:85	shiitake mushroom	69:85	shiitake mushroom (Lentinula edodes)	69:104	Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol.					
30500765	0	38	theme	shiitake	69:76	arg1	edodes					98:103	edodes	98:103	edodes	98:103	Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol.					
30500765	1	39	theme	chemical	294:301	arg1	composition					303:313	chemical composition	294:313	chemical composition of heat-pretreated material	294:341	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	2	40	theme	comparable	558:567	arg1	yield					580:584	higher or comparable fruit-body yield	548:584	higher or comparable fruit-body yield	548:584	Compared to autoclaving, HAP resulted in faster mycelial growth, earlier fructification, and higher or comparable fruit-body yield.					
30500765	4	41	theme	glucan	930:935	arg1	fraction					918:925	the mass fraction	909:925	the mass fraction of glucan	909:935	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	4	42	theme	mass	817:820	arg1	fraction					822:829	the mass fraction	813:829	the mass fraction of lignin to less than half of the initial	813:872	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	4	43	theme	lignin	834:839	arg1	fraction					822:829	the mass fraction	813:829	the mass fraction of lignin to less than half of the initial	813:872	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	1	44	theme	Hot-air	122:128	arg1	HAP					158:160	HAP	158:160	HAP	158:160	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	44	theme	Hot-air	122:128	arg1	pasteurisation					142:155	Hot-air (75-100 °C) pasteurisation	122:155	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate	122:191	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	3	45	from	material	697:704	arg1	SMS					710:712	SMS	710:712	SMS	710:712	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	3	45	from	material	697:704	arg1	fractions					646:654	the fractions	642:654	the fractions of carbohydrate and lignin in pretreated material	642:704	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	3	46	theme	lower	745:749	arg1	fractions					751:759	lower fractions	745:759	lower fractions of extractives	745:774	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	1	47	from	121 °C	244:249	arg1	steam					235:239	steam	235:239	steam at 121 °C	235:249	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	3	48	theme	carbohydrate	659:670	arg1	SMS					710:712	SMS	710:712	SMS	710:712	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	3	48	theme	carbohydrate	659:670	arg1	fractions					646:654	the fractions	642:654	the fractions of carbohydrate and lignin in pretreated material	642:704	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	5	49	theme	heating	1009:1015	arg1	method					1017:1022	heating method	1009:1022	heating method	1009:1022	The choice of heating method did not affect enzymatic digestibility.					
30500765	3	50	theme	pretreated	686:695	arg1	material					697:704	pretreated material	686:704	pretreated material	686:704	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	2	51	theme	higher	548:553	arg1	yield					580:584	higher or comparable fruit-body yield	548:584	higher or comparable fruit-body yield	548:584	Compared to autoclaving, HAP resulted in faster mycelial growth, earlier fructification, and higher or comparable fruit-body yield.					
30500765	1	52	theme	glucan	406:411	arg1	digestibility					389:401	enzymatic digestibility	379:401	enzymatic digestibility of glucan in SMS	379:418	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	52	theme	glucan	406:411	arg1	composition					303:313	chemical composition	294:313	chemical composition of heat-pretreated material	294:341	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	52	theme	glucan	406:411	arg1	substrate					362:370	spent mushroom substrate	347:370	spent mushroom substrate	347:370	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	52	theme	glucan	406:411	arg1	growth					276:281	shiitake growth and yield	267:291	growth	276:281	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	52	theme	glucan	406:411	arg1	SMS					373:375	SMS	373:375	SMS	373:375	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	52	theme	glucan	406:411	arg1	yield					287:291	shiitake growth and yield	267:291	yield	287:291	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	52	theme	glucan	406:411	arg1	yield					448:452	theoretical bioethanol yield	425:452	theoretical bioethanol yield	425:452	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	4	53	theme	Shiitake	777:784	arg1	cultivation					786:796	Shiitake cultivation	777:796	Shiitake cultivation	777:796	Shiitake cultivation, which reduced the mass fraction of lignin to less than half of the initial without having any major impact on the mass fraction of glucan, enhanced enzymatic hydrolysis of glucan about four-fold.					
30500765	5	54	theme	enzymatic	1039:1047	arg1	digestibility					1049:1061	enzymatic digestibility	1039:1061	enzymatic digestibility	1039:1061	The choice of heating method did not affect enzymatic digestibility.					
30500765	1	55	theme	75-100 °C	131:139	arg1	HAP					158:160	HAP	158:160	HAP	158:160	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	55	theme	75-100 °C	131:139	arg1	pasteurisation					142:155	Hot-air (75-100 °C) pasteurisation	122:155	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate	122:191	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	5	56	theme	method	1017:1022	arg1	choice					999:1004	The choice	995:1004	The choice of heating method	995:1022	The choice of heating method did not affect enzymatic digestibility.					
30500765	1	57	dep	autoclaving	222:232	arg1	steam					235:239	steam	235:239	steam at 121 °C	235:249	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	1	58	from	yield	448:452	arg1	SMS					416:418	SMS	416:418	SMS	416:418	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	3	59	from	fractions	646:654	arg1	material					697:704	pretreated material	686:704	pretreated material	686:704	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	3	60	theme	heat	591:594	arg1	pretreatment					596:607	The heat pretreatment methods	587:615	The heat pretreatment methods	587:615	The heat pretreatment methods did not differ regarding the fractions of carbohydrate and lignin in pretreated material and SMS, but HAP typically resulted in lower fractions of extractives.					
30500765	0	61	theme	mushroom	78:85	arg1	production					55:64	combined production	46:64	combined production of shiitake mushroom (Lentinula edodes)	46:104	Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol.					
30500765	0	61	theme	mushroom	78:85	arg1	bioethanol					110:119	bioethanol	110:119	bioethanol	110:119	Energy-efficient substrate pasteurisation for combined production of shiitake mushroom (Lentinula edodes) and bioethanol.					
30500765	1	62	theme	heat-pretreated	318:332	arg1	material					334:341	heat-pretreated material	318:341	heat-pretreated material	318:341	Hot-air (75-100 °C) pasteurisation (HAP) of birch-wood-based substrate was compared to conventional autoclaving (steam at 121 °C) with regard to shiitake growth and yield, chemical composition of heat-pretreated material and spent mushroom substrate (SMS), enzymatic digestibility of glucan in SMS, and theoretical bioethanol yield.					
30500765	2	63	theme	mycelial	503:510	arg1	growth					512:517	faster mycelial growth	496:517	faster mycelial growth	496:517	Compared to autoclaving, HAP resulted in faster mycelial growth, earlier fructification, and higher or comparable fruit-body yield.					
29778838	12	0	theme	fruit	1852:1856	arg1	firmness					1832:1839	firmness	1832:1839	firmness of avocado fruit	1832:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	12	1	theme	firmness	1832:1839	arg1	loss					1824:1827	the loss	1820:1827	the loss of firmness of avocado fruit	1820:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	2	2	theme	storage	298:304	arg1	capacity					306:313	cold storage capacity	293:313	cold storage capacity	293:313	During ripening, the mesocarp changes in texture, and this affects fruit quality and cold storage capacity.					
29778838	0	3	from	relation	39:46	arg1	pectins					21:27	cell wall pectins	11:27	cell wall pectins	11:27	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	0	4	theme	mesocarp	63:70	arg1	softening					72:80	postharvest mesocarp softening	51:80	postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)	51:124	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	4	5	theme	avocado	466:472	arg1	softening					474:482	avocado softening	466:482	avocado softening	466:482	However, changes in the cell wall structure and composition during avocado softening are poorly understood.					
29778838	2	6	theme	cold	293:296	arg1	capacity					306:313	cold storage capacity	293:313	cold storage capacity	293:313	During ripening, the mesocarp changes in texture, and this affects fruit quality and cold storage capacity.					
29778838	11	7	theme	fruit	1378:1382	arg1	storage					1359:1365	The cold storage	1350:1365	The cold storage of avocado fruit	1350:1382	The cold storage of avocado fruit did not induce softening of the fruit, but the content of GalA showed a substantial decrease, accompanied by an increase in PG activity.					
29778838	12	8	theme	sugars	1595:1600	arg1	solubilization					1569:1582	the solubilization	1565:1582	the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase,	1565:1713	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	12	8	theme	sugars	1595:1600	arg1	factors					1729:1735	the main factors	1720:1735	the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit	1720:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	3	9	from	disassembly	364:374	arg1	fruits					391:396	climacteric fruits	379:396	climacteric fruits	379:396	Softening is commonly associated with cell wall disassembly in climacteric fruits.					
29778838	8	10	theme	same	959:962	arg1	way					964:966	the same way	955:966	the same way	955:966	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	10	11	theme	pectin	1286:1291	arg1	fractions					1273:1281	the different fractions	1259:1281	the different fractions of pectin	1259:1291	However, there was a change in arabinose ratios between the different fractions of pectin, mainly in the fractions soluble in water and in Na2CO3.					
29778838	7	12	theme	water-soluble	843:855	arg1	pectin					857:862	water-soluble pectin	843:862	water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity	843:949	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	4	13	theme	cell	423:426	arg1	wall					428:431	the cell wall structure and composition	419:457	the cell wall structure and composition	419:457	However, changes in the cell wall structure and composition during avocado softening are poorly understood.					
29778838	10	14	theme	soluble	1318:1324	arg1	fractions					1308:1316	the fractions	1304:1316	the fractions soluble in water and in Na2CO3	1304:1347	However, there was a change in arabinose ratios between the different fractions of pectin, mainly in the fractions soluble in water and in Na2CO3.					
29778838	5	15	from	pectins	545:551	arg1	fruit					571:575	"Hass" avocado fruit	556:575	"Hass" avocado fruit	556:575	To understand this process, cell wall pectins in "Hass" avocado fruit were studied during ripening at 20 °C after harvest and after cold storage.					
29778838	0	16	theme	"	90:90	arg1	avocados					92:99	"Hass" avocados	85:99	"Hass" avocados (Persea americana Mill.)	85:124	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	0	16	theme	"	90:90	arg1	Mill					119:122	Persea americana Mill.	102:123	Persea americana Mill.	102:123	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	5	17	theme	"	561:561	arg1	fruit					571:575	"Hass" avocado fruit	556:575	"Hass" avocado fruit	556:575	To understand this process, cell wall pectins in "Hass" avocado fruit were studied during ripening at 20 °C after harvest and after cold storage.					
29778838	12	18	theme	neutral	1587:1593	arg1	loss					1649:1652	the loss	1645:1652	the loss of galactose content mediated by the enzyme β-galactosidase	1645:1712	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	12	18	theme	neutral	1587:1593	arg1	rhamnose					1624:1631	rhamnose	1624:1631	rhamnose	1624:1631	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	12	18	theme	neutral	1587:1593	arg1	sugars					1595:1600	neutral sugars	1587:1600	neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase,	1587:1713	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	12	18	theme	neutral	1587:1593	arg1	arabinose					1610:1618	arabinose	1610:1618	arabinose	1610:1618	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	5	19	theme	cold	639:642	arg1	storage					644:650	cold storage	639:650	cold storage	639:650	To understand this process, cell wall pectins in "Hass" avocado fruit were studied during ripening at 20 °C after harvest and after cold storage.					
29778838	8	20	theme	cell	1070:1073	arg1	material					1080:1087	cell wall material	1070:1087	cell wall material	1070:1087	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	11	21	theme	PG	1508:1509	arg1	activity					1511:1518	PG activity	1508:1518	PG activity	1508:1518	The cold storage of avocado fruit did not induce softening of the fruit, but the content of GalA showed a substantial decrease, accompanied by an increase in PG activity.					
29778838	5	22	theme	avocado	563:569	arg1	fruit					571:575	"Hass" avocado fruit	556:575	"Hass" avocado fruit	556:575	To understand this process, cell wall pectins in "Hass" avocado fruit were studied during ripening at 20 °C after harvest and after cold storage.					
29778838	7	23	theme	concomitant	864:874	arg1	pectin					857:862	water-soluble pectin	843:862	water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity	843:949	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	12	24	theme	remodeling	1784:1793	arg1	enzymes					1795:1801	cell wall remodeling enzymes	1774:1801	cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit	1774:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	7	25	theme	galacturonic	778:789	arg1	acid					791:794	galacturonic acid	778:794	galacturonic acid (GalA)	778:801	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	7	25	theme	galacturonic	778:789	arg1	GalA					797:800	GalA	797:800	GalA	797:800	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	7	26	from	increase	907:914	arg1	activity					942:949	polygalacturonase (PG) activity	919:949	polygalacturonase (PG) activity	919:949	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	12	27	theme	enzymes	1795:1801	arg1	action					1764:1769	the coordinated action	1748:1769	the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit	1748:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	8	28	from	fraction	1112:1119	arg1	material					1080:1087	cell wall material	1070:1087	cell wall material	1070:1087	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	8	28	from	fraction	1112:1119	arg1	galactose					1057:1065	galactose	1057:1065	galactose	1057:1065	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	8	29	theme	β-galactosidase	973:987	arg1	activity					989:996	the β-galactosidase activity	969:996	the β-galactosidase activity	969:996	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	12	30	theme	galactose	1657:1665	arg1	content					1667:1673	galactose content	1657:1673	galactose content mediated by the enzyme β-galactosidase	1657:1712	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	8	31	theme	soft	1011:1014	arg1	fruit					1024:1028	soft avocado fruit	1011:1028	soft avocado fruit	1011:1028	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	7	32	theme	Biochemical	736:746	arg1	analysis					748:755	Biochemical analysis	736:755	Biochemical analysis	736:755	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	12	33	theme	main	1724:1727	arg1	solubilization					1569:1582	the solubilization	1565:1582	the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase,	1565:1713	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	12	33	theme	main	1724:1727	arg1	factors					1729:1735	the main factors	1720:1735	the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit	1720:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	5	34	theme	cell	535:538	arg1	pectins					545:551	cell wall pectins	535:551	cell wall pectins in "Hass" avocado fruit	535:575	To understand this process, cell wall pectins in "Hass" avocado fruit were studied during ripening at 20 °C after harvest and after cold storage.					
29778838	3	35	theme	climacteric	379:389	arg1	fruits					391:396	climacteric fruits	379:396	climacteric fruits	379:396	Softening is commonly associated with cell wall disassembly in climacteric fruits.					
29778838	0	36	theme	wall	16:19	arg1	pectins					21:27	cell wall pectins	11:27	cell wall pectins	11:27	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	4	37	dep	wall	428:431	arg1	composition					447:457	composition	447:457	composition	447:457	However, changes in the cell wall structure and composition during avocado softening are poorly understood.					
29778838	4	37	dep	wall	428:431	arg1	structure					433:441	structure	433:441	structure	433:441	However, changes in the cell wall structure and composition during avocado softening are poorly understood.					
29778838	12	38	theme	enzyme	1691:1696	arg1	β-galactosidase					1698:1712	the enzyme β-galactosidase	1687:1712	the enzyme β-galactosidase	1687:1712	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	5	39	theme	wall	540:543	arg1	pectins					545:551	cell wall pectins	535:551	cell wall pectins in "Hass" avocado fruit	535:575	To understand this process, cell wall pectins in "Hass" avocado fruit were studied during ripening at 20 °C after harvest and after cold storage.					
29778838	0	40	theme	cell	11:14	arg1	pectins					21:27	cell wall pectins	11:27	cell wall pectins	11:27	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	0	41	theme	avocados	92:99	arg1	softening					72:80	postharvest mesocarp softening	51:80	postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)	51:124	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	3	42	theme	wall	359:362	arg1	disassembly					364:374	cell wall disassembly	354:374	cell wall disassembly in climacteric fruits	354:396	Softening is commonly associated with cell wall disassembly in climacteric fruits.					
29778838	10	43	from	water	1329:1333	arg1	soluble					1318:1324	soluble	1318:1324	soluble	1318:1324	However, there was a change in arabinose ratios between the different fractions of pectin, mainly in the fractions soluble in water and in Na2CO3.					
29778838	0	44	theme	americana	109:117	arg1	avocados					92:99	"Hass" avocados	85:99	"Hass" avocados (Persea americana Mill.)	85:124	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	0	44	theme	americana	109:117	arg1	Mill					119:122	Persea americana Mill.	102:123	Persea americana Mill.	102:123	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	8	45	from	reduction	1044:1052	arg1	material					1080:1087	cell wall material	1070:1087	cell wall material	1070:1087	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	8	45	from	reduction	1044:1052	arg1	galactose					1057:1065	galactose	1057:1065	galactose	1057:1065	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	12	46	theme	cell	1774:1777	arg1	enzymes					1795:1801	cell wall remodeling enzymes	1774:1801	cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit	1774:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	1	47	theme	climacteric	144:154	arg1	avocado					131:137	The avocado	127:137	The avocado	127:137	The avocado is a climacteric fruit and begins a softening process after harvest.					
29778838	1	47	theme	climacteric	144:154	arg1	fruit					156:160	a climacteric fruit	142:160	a climacteric fruit	142:160	The avocado is a climacteric fruit and begins a softening process after harvest.					
29778838	9	48	from	content	1136:1142	arg1	material					1161:1168	the cell wall material	1147:1168	the cell wall material	1147:1168	The arabinose content in the cell wall material did not change during softening.					
29778838	10	49	from	change	1224:1229	arg1	ratios					1244:1249	arabinose ratios	1234:1249	arabinose ratios	1234:1249	However, there was a change in arabinose ratios between the different fractions of pectin, mainly in the fractions soluble in water and in Na2CO3.					
29778838	10	50	from	Na2CO3	1342:1347	arg1	soluble					1318:1324	soluble	1318:1324	soluble	1318:1324	However, there was a change in arabinose ratios between the different fractions of pectin, mainly in the fractions soluble in water and in Na2CO3.					
29778838	0	51	theme	Persea	102:107	arg1	avocados					92:99	"Hass" avocados	85:99	"Hass" avocados (Persea americana Mill.)	85:124	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	0	51	theme	Persea	102:107	arg1	Mill					119:122	Persea americana Mill.	102:123	Persea americana Mill.	102:123	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	8	52	theme	Na2CO3-soluble	1097:1110	arg1	fraction					1112:1119	the Na2CO3-soluble fraction	1093:1119	the Na2CO3-soluble fraction	1093:1119	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	10	53	theme	arabinose	1234:1242	arg1	ratios					1244:1249	arabinose ratios	1234:1249	arabinose ratios	1234:1249	However, there was a change in arabinose ratios between the different fractions of pectin, mainly in the fractions soluble in water and in Na2CO3.					
29778838	6	54	from	delay	716:720	arg1	softening					725:733	softening	725:733	softening	725:733	Additionally, avocados were treated with 1-MCP to evaluate the delay in softening.					
29778838	11	55	from	increase	1496:1503	arg1	activity					1511:1518	PG activity	1508:1518	PG activity	1508:1518	The cold storage of avocado fruit did not induce softening of the fruit, but the content of GalA showed a substantial decrease, accompanied by an increase in PG activity.					
29778838	12	56	theme	content	1667:1673	arg1	loss					1649:1652	the loss	1645:1652	the loss of galactose content mediated by the enzyme β-galactosidase	1645:1712	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	12	56	theme	content	1667:1673	arg1	rhamnose					1624:1631	rhamnose	1624:1631	rhamnose	1624:1631	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	12	56	theme	content	1667:1673	arg1	arabinose					1610:1618	arabinose	1610:1618	arabinose	1610:1618	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	9	57	theme	cell	1151:1154	arg1	material					1161:1168	the cell wall material	1147:1168	the cell wall material	1147:1168	The arabinose content in the cell wall material did not change during softening.					
29778838	7	58	from	decrease	766:773	arg1	acid					791:794	galacturonic acid	778:794	galacturonic acid (GalA)	778:801	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	7	58	from	decrease	766:773	arg1	residues					824:831	alcohol-insoluble residues	806:831	alcohol-insoluble residues (AIR)	806:837	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	7	58	from	decrease	766:773	arg1	AIR					834:836	AIR	834:836	AIR	834:836	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	7	58	from	decrease	766:773	arg1	GalA					797:800	GalA	797:800	GalA	797:800	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	4	59	from	changes	408:414	arg1	wall					428:431	the cell wall structure and composition	419:457	the cell wall structure and composition	419:457	However, changes in the cell wall structure and composition during avocado softening are poorly understood.					
29778838	3	60	theme	cell	354:357	arg1	disassembly					364:374	cell wall disassembly	354:374	cell wall disassembly in climacteric fruits	354:396	Softening is commonly associated with cell wall disassembly in climacteric fruits.					
29778838	8	61	theme	avocado	1016:1022	arg1	fruit					1024:1028	soft avocado fruit	1011:1028	soft avocado fruit	1011:1028	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	9	62	theme	wall	1156:1159	arg1	material					1161:1168	the cell wall material	1147:1168	the cell wall material	1147:1168	The arabinose content in the cell wall material did not change during softening.					
29778838	11	63	theme	fruit	1416:1420	arg1	softening					1399:1407	softening	1399:1407	softening of the fruit	1399:1420	The cold storage of avocado fruit did not induce softening of the fruit, but the content of GalA showed a substantial decrease, accompanied by an increase in PG activity.					
29778838	7	64	from	pectin	857:862	arg1	acid					791:794	galacturonic acid	778:794	galacturonic acid (GalA)	778:801	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	7	64	from	pectin	857:862	arg1	residues					824:831	alcohol-insoluble residues	806:831	alcohol-insoluble residues (AIR)	806:837	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	7	64	from	pectin	857:862	arg1	AIR					834:836	AIR	834:836	AIR	834:836	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	7	64	from	pectin	857:862	arg1	GalA					797:800	GalA	797:800	GalA	797:800	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	9	65	theme	arabinose	1126:1134	arg1	content					1136:1142	The arabinose content	1122:1142	The arabinose content in the cell wall material	1122:1168	The arabinose content in the cell wall material did not change during softening.					
29778838	7	66	theme	PG	938:939	arg1	activity					942:949	polygalacturonase (PG) activity	919:949	polygalacturonase (PG) activity	919:949	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	2	67	theme	fruit	275:279	arg1	quality					281:287	fruit quality	275:287	fruit quality	275:287	During ripening, the mesocarp changes in texture, and this affects fruit quality and cold storage capacity.					
29778838	10	68	from	soluble	1318:1324	arg1	water					1329:1333	water	1329:1333	water	1329:1333	However, there was a change in arabinose ratios between the different fractions of pectin, mainly in the fractions soluble in water and in Na2CO3.					
29778838	10	68	from	soluble	1318:1324	arg1	Na2CO3					1342:1347	Na2CO3	1342:1347	Na2CO3	1342:1347	However, there was a change in arabinose ratios between the different fractions of pectin, mainly in the fractions soluble in water and in Na2CO3.					
29778838	1	69	theme	softening	175:183	arg1	process					185:191	a softening process	173:191	a softening process after harvest	173:205	The avocado is a climacteric fruit and begins a softening process after harvest.					
29778838	11	70	theme	GalA	1442:1445	arg1	content					1431:1437	the content	1427:1437	the content of GalA	1427:1445	The cold storage of avocado fruit did not induce softening of the fruit, but the content of GalA showed a substantial decrease, accompanied by an increase in PG activity.					
29778838	8	71	theme	wall	1075:1078	arg1	material					1080:1087	cell wall material	1070:1087	cell wall material	1070:1087	In the same way, the β-galactosidase activity increased in soft avocado fruit, along with a reduction in galactose in cell wall material and the Na2CO3-soluble fraction.					
29778838	10	72	theme	different	1263:1271	arg1	fractions					1273:1281	the different fractions	1259:1281	the different fractions of pectin	1259:1291	However, there was a change in arabinose ratios between the different fractions of pectin, mainly in the fractions soluble in water and in Na2CO3.					
29778838	11	73	theme	cold	1354:1357	arg1	storage					1359:1365	The cold storage	1350:1365	The cold storage of avocado fruit	1350:1382	The cold storage of avocado fruit did not induce softening of the fruit, but the content of GalA showed a substantial decrease, accompanied by an increase in PG activity.					
29778838	0	74	theme	postharvest	51:61	arg1	softening					72:80	postharvest mesocarp softening	51:80	postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)	51:124	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	7	75	theme	polygalacturonase	919:935	arg1	activity					942:949	polygalacturonase (PG) activity	919:949	polygalacturonase (PG) activity	919:949	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	12	76	theme	coordinated	1752:1762	arg1	action					1764:1769	the coordinated action	1748:1769	the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit	1748:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	11	77	theme	avocado	1370:1376	arg1	fruit					1378:1382	avocado fruit	1370:1382	avocado fruit	1370:1382	The cold storage of avocado fruit did not induce softening of the fruit, but the content of GalA showed a substantial decrease, accompanied by an increase in PG activity.					
29778838	12	78	theme	wall	1779:1782	arg1	enzymes					1795:1801	cell wall remodeling enzymes	1774:1801	cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit	1774:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	12	79	theme	avocado	1844:1850	arg1	fruit					1852:1856	avocado fruit	1844:1856	avocado fruit	1844:1856	Thus, our work supports the hypothesis that the solubilization of neutral sugars such as arabinose and rhamnose, as well as the loss of galactose content mediated by the enzyme β-galactosidase, were the main factors that began the coordinated action of cell wall remodeling enzymes that resulted in the loss of firmness of avocado fruit.					
29778838	0	80	from	Changes	0:6	arg1	pectins					21:27	cell wall pectins	11:27	cell wall pectins	11:27	Changes in cell wall pectins and their relation to postharvest mesocarp softening of "Hass" avocados (Persea americana Mill.)					
29778838	11	81	theme	substantial	1456:1466	arg1	decrease					1468:1475	a substantial decrease	1454:1475	a substantial decrease	1454:1475	The cold storage of avocado fruit did not induce softening of the fruit, but the content of GalA showed a substantial decrease, accompanied by an increase in PG activity.					
29778838	7	82	theme	alcohol-insoluble	806:822	arg1	AIR					834:836	AIR	834:836	AIR	834:836	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
29778838	7	82	theme	alcohol-insoluble	806:822	arg1	residues					824:831	alcohol-insoluble residues	806:831	alcohol-insoluble residues (AIR)	806:837	Biochemical analysis showed a decrease in galacturonic acid (GalA) in alcohol-insoluble residues (AIR) and water-soluble pectin concomitant to softening, paralleled by an increase in polygalacturonase (PG) activity.					
30369607	3	0	from	composition	351:361	arg1	menisci					435:441	human OA menisci	426:441	human OA menisci	426:441	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	8	1	theme	meniscus	950:957	arg1	layers					936:941	intact superficial layers	917:941	intact superficial layers of the meniscus	917:957	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	1	2	theme	single	132:137	arg1	structure					153:161	a single chondron-like structure	130:161	a single chondron-like structure	130:161	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	9	3	theme	cell	1064:1067	arg1	clusters					1069:1076	cell clusters	1064:1076	cell clusters	1064:1076	In addition, a substantial difference was detected between cluster areas and degenerative areas without cell clusters.					
30369607	1	4	theme	OA	229:230	arg1	meniscus					233:240	the osteoarthritic (OA) meniscus	209:240	the osteoarthritic (OA) meniscus	209:240	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	0	5	theme	Matrix	69:74	arg1	Components					76:85	Their Extracellular Matrix Components	49:85	Their Extracellular Matrix Components	49:85	Composition of Cell Clusters in Torn Menisci and Their Extracellular Matrix Components.					
30369607	5	6	theme	Sry-type	619:626	arg1	SOX9					639:642	SOX9	639:642	SOX9	639:642	The specimens were subjected to safranin O staining and immunostaining for Sry-type HMG box 9 (SOX9), type II collagen, and aggrecan.					
30369607	5	6	theme	Sry-type	619:626	arg1	box					632:634	Sry-type HMG box 9	619:636	Sry-type HMG box 9 (SOX9)	619:643	The specimens were subjected to safranin O staining and immunostaining for Sry-type HMG box 9 (SOX9), type II collagen, and aggrecan.					
30369607	1	7	theme	chondron-like	139:151	arg1	structure					153:161	a single chondron-like structure	130:161	a single chondron-like structure	130:161	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	0	8	from	Composition	0:10	arg1	Components					76:85	Their Extracellular Matrix Components	49:85	Their Extracellular Matrix Components	49:85	Composition of Cell Clusters in Torn Menisci and Their Extracellular Matrix Components.					
30369607	0	8	from	Composition	0:10	arg1	Menisci					37:43	Torn Menisci	32:43	Torn Menisci	32:43	Composition of Cell Clusters in Torn Menisci and Their Extracellular Matrix Components.					
30369607	5	9	theme	HMG	628:630	arg1	SOX9					639:642	SOX9	639:642	SOX9	639:642	The specimens were subjected to safranin O staining and immunostaining for Sry-type HMG box 9 (SOX9), type II collagen, and aggrecan.					
30369607	5	9	theme	HMG	628:630	arg1	box					632:634	Sry-type HMG box 9	619:636	Sry-type HMG box 9 (SOX9)	619:643	The specimens were subjected to safranin O staining and immunostaining for Sry-type HMG box 9 (SOX9), type II collagen, and aggrecan.					
30369607	2	10	from	clusters	289:296	arg1	humans					301:306	humans	301:306	humans	301:306	However, little is known about these meniscal clusters in humans.					
30369607	10	11	from	uniformity	1167:1176	arg1	menisci					1181:1187	menisci	1181:1187	menisci exposed to a broad range of injuries	1181:1224	We demonstrated that cell clusters localized near fissures and clefts showed remarkable uniformity in menisci exposed to a broad range of injuries.					
30369607	9	12	theme	substantial	975:985	arg1	difference					987:996	a substantial difference	973:996	a substantial difference	973:996	In addition, a substantial difference was detected between cluster areas and degenerative areas without cell clusters.					
30369607	1	13	theme	meniscus	233:240	arg1	areas					200:204	degenerated areas	188:204	degenerated areas of the osteoarthritic (OA) meniscus	188:240	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	8	14	theme	intact	917:922	arg1	layers					936:941	intact superficial layers	917:941	intact superficial layers of the meniscus	917:957	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	5	15	theme	safranin	576:583	arg1	O					585:585	safranin O staining and immunostaining	576:613	O	585:585	The specimens were subjected to safranin O staining and immunostaining for Sry-type HMG box 9 (SOX9), type II collagen, and aggrecan.					
30369607	7	16	theme	tissue	801:806	arg1	sample					808:813	each tissue sample	796:813	each tissue sample	796:813	Five regions of interest were analyzed within each tissue sample.					
30369607	3	17	theme	deposition	391:400	arg1	composition					351:361	the composition	347:361	the composition of the extracellular matrix deposition around cell clusters in human OA menisci	347:441	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	4	18	theme	Twenty-six	444:453	arg1	menisci					455:461	Twenty-six menisci	444:461	Twenty-six menisci	444:461	Twenty-six menisci were obtained through total knee arthroplasty from patients with OA knee joints.					
30369607	8	19	theme	superficial	924:934	arg1	layers					936:941	intact superficial layers	917:941	intact superficial layers of the meniscus	917:957	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	4	20	with	patients	514:521	arg1	joints					536:541	OA knee joints	528:541	OA knee joints	528:541	Twenty-six menisci were obtained through total knee arthroplasty from patients with OA knee joints.					
30369607	8	21	theme	SOX9	820:823	arg1	densities					857:865	The SOX9, type II collagen, and aggrecan densities	816:865	The SOX9, type II collagen, and aggrecan densities	816:865	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	8	21	theme	SOX9	820:823	arg1	higher					885:890	higher	885:890	higher	885:890	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	11	22	dep	proteins	1257:1264	arg1	SOX9					1266:1269	SOX9	1266:1269	SOX9	1266:1269	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	11	22	dep	proteins	1257:1264	arg1	collagen					1280:1287	type II collagen	1272:1287	type II collagen	1272:1287	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	11	22	dep	proteins	1257:1264	arg1	aggrecan					1294:1301	aggrecan	1294:1301	aggrecan	1294:1301	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	11	22	dep	proteins	1257:1264	arg1	proteins					1257:1264	the chondrogenic proteins SOX9, type II collagen, and aggrecan	1240:1301	the chondrogenic proteins SOX9, type II collagen, and aggrecan	1240:1301	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	3	23	from	menisci	435:441	arg1	composition					351:361	the composition	347:361	the composition of the extracellular matrix deposition around cell clusters in human OA menisci	347:441	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	2	24	theme	meniscal	280:287	arg1	clusters					289:296	these meniscal clusters	274:296	these meniscal clusters in humans	274:306	However, little is known about these meniscal clusters in humans.					
30369607	3	25	theme	extracellular	370:382	arg1	deposition					391:400	the extracellular matrix deposition	366:400	the extracellular matrix deposition around cell clusters in human OA menisci	366:441	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	1	26	theme	Cell	88:91	arg1	clusters					93:100	Cell clusters	88:100	Cell clusters	88:100	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	7	27	theme	interest	766:773	arg1	regions					755:761	Five regions	750:761	Five regions of interest	750:773	Five regions of interest were analyzed within each tissue sample.					
30369607	3	28	theme	matrix	384:389	arg1	deposition					391:400	the extracellular matrix deposition	366:400	the extracellular matrix deposition around cell clusters in human OA menisci	366:441	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	3	29	theme	study	324:328	arg1	aim					313:315	The aim	309:315	The aim of our study	309:328	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	10	30	theme	injuries	1217:1224	arg1	range					1208:1212	a broad range	1200:1212	a broad range of injuries	1200:1224	We demonstrated that cell clusters localized near fissures and clefts showed remarkable uniformity in menisci exposed to a broad range of injuries.					
30369607	6	31	theme	ImageJ	733:738	arg1	software					740:747	ImageJ software	733:747	ImageJ software	733:747	Their signal density after staining was assessed using ImageJ software.					
30369607	11	32	theme	chondrogenic	1244:1255	arg1	SOX9					1266:1269	SOX9	1266:1269	SOX9	1266:1269	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	11	32	theme	chondrogenic	1244:1255	arg1	collagen					1280:1287	type II collagen	1272:1287	type II collagen	1272:1287	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	11	32	theme	chondrogenic	1244:1255	arg1	proteins					1257:1264	the chondrogenic proteins SOX9, type II collagen, and aggrecan	1240:1301	the chondrogenic proteins SOX9, type II collagen, and aggrecan	1240:1301	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	11	32	theme	chondrogenic	1244:1255	arg1	aggrecan					1294:1301	aggrecan	1294:1301	aggrecan	1294:1301	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	0	33	theme	Cell	15:18	arg1	Clusters					20:27	Cell Clusters	15:27	Cell Clusters	15:27	Composition of Cell Clusters in Torn Menisci and Their Extracellular Matrix Components.					
30369607	4	34	theme	knee	531:534	arg1	joints					536:541	OA knee joints	528:541	OA knee joints	528:541	Twenty-six menisci were obtained through total knee arthroplasty from patients with OA knee joints.					
30369607	5	35	theme	type	646:649	arg1	collagen					654:661	type II collagen	646:661	type II collagen	646:661	The specimens were subjected to safranin O staining and immunostaining for Sry-type HMG box 9 (SOX9), type II collagen, and aggrecan.					
30369607	8	36	theme	collagen	834:841	arg1	densities					857:865	The SOX9, type II collagen, and aggrecan densities	816:865	The SOX9, type II collagen, and aggrecan densities	816:865	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	8	36	theme	collagen	834:841	arg1	higher					885:890	higher	885:890	higher	885:890	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	3	37	theme	human	426:430	arg1	menisci					435:441	human OA menisci	426:441	human OA menisci	426:441	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	0	38	theme	Torn	32:35	arg1	Menisci					37:43	Torn Menisci	32:43	Torn Menisci	32:43	Composition of Cell Clusters in Torn Menisci and Their Extracellular Matrix Components.					
30369607	4	39	theme	OA	528:529	arg1	joints					536:541	OA knee joints	528:541	OA knee joints	528:541	Twenty-six menisci were obtained through total knee arthroplasty from patients with OA knee joints.					
30369607	1	40	theme	degenerated	188:198	arg1	areas					200:204	degenerated areas	188:204	degenerated areas of the osteoarthritic (OA) meniscus	188:240	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	9	41	theme	degenerative	1037:1048	arg1	areas					1050:1054	degenerative areas	1037:1054	degenerative areas	1037:1054	In addition, a substantial difference was detected between cluster areas and degenerative areas without cell clusters.					
30369607	3	42	theme	cell	409:412	arg1	clusters					414:421	cell clusters	409:421	cell clusters	409:421	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	4	43	theme	knee	491:494	arg1	arthroplasty					496:507	total knee arthroplasty	485:507	total knee arthroplasty	485:507	Twenty-six menisci were obtained through total knee arthroplasty from patients with OA knee joints.					
30369607	9	44	theme	cluster	1019:1025	arg1	areas					1027:1031	cluster areas	1019:1031	cluster areas	1019:1031	In addition, a substantial difference was detected between cluster areas and degenerative areas without cell clusters.					
30369607	0	45	theme	Clusters	20:27	arg1	Composition					0:10	Composition	0:10	Composition of Cell Clusters in Torn Menisci and Their Extracellular Matrix Components.	0:86	Composition of Cell Clusters in Torn Menisci and Their Extracellular Matrix Components.					
30369607	5	46	dep	O	585:585	arg1	staining					587:594	staining	587:594	staining	587:594	The specimens were subjected to safranin O staining and immunostaining for Sry-type HMG box 9 (SOX9), type II collagen, and aggrecan.					
30369607	4	47	theme	total	485:489	arg1	arthroplasty					496:507	total knee arthroplasty	485:507	total knee arthroplasty	485:507	Twenty-six menisci were obtained through total knee arthroplasty from patients with OA knee joints.					
30369607	9	48	located	detected	1002:1009	arg2	difference					987:996	a substantial difference	973:996	a substantial difference	973:996	In addition, a substantial difference was detected between cluster areas and degenerative areas without cell clusters.					
30369607	9	48	located	detected	1002:1009	arg1	addition					963:970	addition	963:970	addition	963:970	In addition, a substantial difference was detected between cluster areas and degenerative areas without cell clusters.					
30369607	8	49	theme	type	826:829	arg1	collagen					834:841	type II collagen	826:841	type II collagen	826:841	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	6	50	theme	signal	684:689	arg1	density					691:697	Their signal density	678:697	Their signal density after staining	678:712	Their signal density after staining was assessed using ImageJ software.					
30369607	1	51	located	found	179:183	arg2	clusters					93:100	Cell clusters	88:100	Cell clusters	88:100	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	1	51	located	found	179:183	arg2	cells					116:120	cells	116:120	cells sharing a single chondron-like structure	116:161	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	1	51	located	found	179:183	arg2	groups					106:111	groups	106:111	groups of cells sharing a single chondron-like structure	106:161	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	1	51	located	found	179:183	arg1	areas					200:204	degenerated areas	188:204	degenerated areas of the osteoarthritic (OA) meniscus	188:240	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	8	52	theme	cluster	895:901	arg1	areas					903:907	cluster areas	895:907	cluster areas	895:907	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	3	53	theme	OA	432:433	arg1	menisci					435:441	human OA menisci	426:441	human OA menisci	426:441	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	8	54	theme	aggrecan	848:855	arg1	densities					857:865	The SOX9, type II collagen, and aggrecan densities	816:865	The SOX9, type II collagen, and aggrecan densities	816:865	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	8	54	theme	aggrecan	848:855	arg1	higher					885:890	higher	885:890	higher	885:890	The SOX9, type II collagen, and aggrecan densities were considerably higher in cluster areas than in intact superficial layers of the meniscus.					
30369607	1	55	theme	cells	116:120	arg1	cells					116:120	cells	116:120	cells sharing a single chondron-like structure	116:161	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	1	55	theme	cells	116:120	arg1	clusters					93:100	Cell clusters	88:100	Cell clusters	88:100	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	1	55	theme	cells	116:120	arg1	groups					106:111	groups	106:111	groups of cells sharing a single chondron-like structure	106:161	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	0	56	theme	Extracellular	55:67	arg1	Components					76:85	Their Extracellular Matrix Components	49:85	Their Extracellular Matrix Components	49:85	Composition of Cell Clusters in Torn Menisci and Their Extracellular Matrix Components.					
30369607	10	57	theme	remarkable	1156:1165	arg1	uniformity					1167:1176	remarkable uniformity	1156:1176	remarkable uniformity in menisci exposed to a broad range of injuries	1156:1224	We demonstrated that cell clusters localized near fissures and clefts showed remarkable uniformity in menisci exposed to a broad range of injuries.					
30369607	1	58	theme	osteoarthritic	213:226	arg1	meniscus					233:240	the osteoarthritic (OA) meniscus	209:240	the osteoarthritic (OA) meniscus	209:240	Cell clusters, or groups of cells sharing a single chondron-like structure, are frequently found in degenerated areas of the osteoarthritic (OA) meniscus.					
30369607	11	59	theme	type	1272:1275	arg1	collagen					1280:1287	type II collagen	1272:1287	type II collagen	1272:1287	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	11	59	theme	type	1272:1275	arg1	proteins					1257:1264	the chondrogenic proteins SOX9, type II collagen, and aggrecan	1240:1301	the chondrogenic proteins SOX9, type II collagen, and aggrecan	1240:1301	In addition, the chondrogenic proteins SOX9, type II collagen, and aggrecan were highly expressed in these tissues.					
30369607	3	60	from	deposition	391:400	arg1	menisci					435:441	human OA menisci	426:441	human OA menisci	426:441	The aim of our study was to determine the composition of the extracellular matrix deposition around cell clusters in human OA menisci.					
30369607	10	61	theme	broad	1202:1206	arg1	range					1208:1212	a broad range	1200:1212	a broad range of injuries	1200:1224	We demonstrated that cell clusters localized near fissures and clefts showed remarkable uniformity in menisci exposed to a broad range of injuries.					
30369607	10	62	theme	cell	1100:1103	arg1	clusters					1105:1112	cell clusters	1100:1112	cell clusters localized near fissures and clefts	1100:1147	We demonstrated that cell clusters localized near fissures and clefts showed remarkable uniformity in menisci exposed to a broad range of injuries.					
29778476	12	0	theme	force	1942:1946	arg1	microscopy					1948:1957	atomic force microscopy	1935:1957	atomic force microscopy images	1935:1964	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	12	1	theme	surface	2045:2051	arg1	coverage					2053:2060	extensive protein surface coverage	2027:2060	extensive protein surface coverage	2027:2060	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	10	2	theme	DX	1623:1624	arg1	MW					1617:1618	the MW	1613:1618	the MW of DX	1613:1624	With the increase in the MW of DX, there was an increase in the MW values of the corresponding glycates but a decrease in the number of bound DX.					
29778476	0	3	theme	milk	157:160	arg1	allergy					170:176	cow milk protein allergy	153:176	cow milk protein allergy	153:176	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	0	4	with	Effect	65:70	arg1	sera					120:123	blood sera	114:123	blood sera obtained from patients with cow milk protein allergy	114:176	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	2	5	theme	protein	320:326	arg1	allergenicity					328:340	protein allergenicity	320:340	protein allergenicity	320:340	One potential approach to decrease protein allergenicity is to block IgE-binding epitopes of the protein allergen by attachment of polysaccharides via the Maillard reaction (i.e., glycation).					
29778476	9	6	theme	1	1495:1495	arg1	values					1485:1490	MW values	1482:1490	MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa	1482:1535	The WPI to DX molar ratios in the glycates made from DX that had MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa were 1:4, 1:3, 1:2, 1:1.5, 1:1, and 1:1, respectively.					
29778476	2	7	dep	approach	299:306	arg1	decrease					311:318	decrease	311:318	to decrease protein allergenicity	308:340	One potential approach to decrease protein allergenicity is to block IgE-binding epitopes of the protein allergen by attachment of polysaccharides via the Maillard reaction (i.e., glycation).					
29778476	13	8	theme	greater	2195:2201	arg1	barrier					2235:2241	a physical barrier	2224:2241	a physical barrier	2224:2241	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	13	8	theme	greater	2195:2201	arg1	hindrance					2210:2218	greater steric hindrance	2195:2218	greater steric hindrance (or a physical barrier)	2195:2242	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	13	9	theme	G10	2165:2167	arg1	glycate					2169:2175	the G10 glycate	2161:2175	the G10 glycate	2161:2175	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	13	10	theme	physical	2226:2233	arg1	barrier					2235:2241	a physical barrier	2224:2241	a physical barrier	2224:2241	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	13	10	theme	physical	2226:2233	arg1	hindrance					2210:2218	greater steric hindrance	2195:2218	greater steric hindrance (or a physical barrier)	2195:2242	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	4	11	theme	milk	649:652	arg1	allergy					662:668	cow milk protein allergy	645:668	cow milk protein allergy	645:668	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	7	12	theme	Blood	1014:1018	arg1	sera					1020:1023	Blood sera	1014:1023	Blood sera	1014:1023	Blood sera were obtained from 8 patients who had been diagnosed with cow milk protein allergy, and a composite sera sample was used for IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method.					
29778476	5	13	theme	Maillard	826:833	arg1	reaction					835:842	the Maillard reaction	822:842	the Maillard reaction performed in aqueous solutions	822:873	Glycation was performed using the initial step of the Maillard reaction performed in aqueous solutions.					
29778476	11	14	theme	WPI-DX	1742:1747	arg1	glycates					1749:1756	The WPI-DX glycates	1738:1756	The WPI-DX glycates	1738:1756	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	12	15	theme	whey	2122:2125	arg1	protein					2127:2133	whey protein	2122:2133	whey protein	2122:2133	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	4	16	theme	IgE	616:618	arg1	binding					620:626	IgE binding	616:626	IgE binding	616:626	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	10	17	theme	MW	1656:1657	arg1	values					1659:1664	the MW values	1652:1664	the MW values of the corresponding glycates	1652:1694	With the increase in the MW of DX, there was an increase in the MW values of the corresponding glycates but a decrease in the number of bound DX.					
29778476	13	18	theme	IgE	2146:2148	arg1	due					2188:2190	due	2188:2190	due	2188:2190	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	13	18	theme	IgE	2146:2148	arg1	binding					2150:2156	The lower IgE binding	2136:2156	The lower IgE binding of the G10 glycate	2136:2175	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	3	19	theme	various	534:540	arg1	properties					553:562	various functional properties	534:562	various functional properties	534:562	Protein glycation has been extensively studied to modify various functional properties.					
29778476	1	20	theme	growing	181:187	arg1	concern					189:195	A growing concern	179:195	A growing concern around the world	179:212	A growing concern around the world is the number of people who are suffering from food protein allergies.					
29778476	1	20	theme	growing	181:187	arg1	number					221:226	the number	217:226	the number of people who are suffering from food protein allergies	217:282	A growing concern around the world is the number of people who are suffering from food protein allergies.					
29778476	14	21	theme	protein	2422:2428	arg1	allergenicity					2400:2412	the allergenicity	2396:2412	the allergenicity of whey protein	2396:2428	In summary, our results demonstrate that glycating WPI with DX via Maillard reaction can potentially be used to decrease the allergenicity of whey protein.					
29778476	9	22	contain	had	1478:1480	arg2	values					1485:1490	MW values	1482:1490	MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa	1482:1535	The WPI to DX molar ratios in the glycates made from DX that had MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa were 1:4, 1:3, 1:2, 1:1.5, 1:1, and 1:1, respectively.					
29778476	9	22	contain	had	1478:1480	arg1	DX					1470:1471	DX	1470:1471	DX that had MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa	1470:1535	The WPI to DX molar ratios in the glycates made from DX that had MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa were 1:4, 1:3, 1:2, 1:1.5, 1:1, and 1:1, respectively.					
29778476	14	23	used	used	2379:2382	arg2	WPI					2326:2328	glycating WPI	2316:2328	glycating WPI with DX via Maillard reaction	2316:2358	In summary, our results demonstrate that glycating WPI with DX via Maillard reaction can potentially be used to decrease the allergenicity of whey protein.					
29778476	5	24	theme	initial	806:812	arg1	step					814:817	the initial step	802:817	the initial step of the Maillard reaction performed in aqueous solutions	802:873	Glycation was performed using the initial step of the Maillard reaction performed in aqueous solutions.					
29778476	11	25	theme	whey	1768:1771	arg1	capacity					1793:1800	lower whey protein IgE-binding capacity	1762:1800	lower whey protein IgE-binding capacity	1762:1800	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	10	26	theme	corresponding	1673:1685	arg1	glycates					1687:1694	the corresponding glycates	1669:1694	the corresponding glycates	1669:1694	With the increase in the MW of DX, there was an increase in the MW values of the corresponding glycates but a decrease in the number of bound DX.					
29778476	4	27	dep	mass	705:708	arg1	MW					711:712	MW	711:712	molar mass; MW	699:712	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	8	28	theme	glycates	1309:1316	arg1	MW					1294:1295	the MW	1290:1295	the MW of purified glycates	1290:1316	The WPI was glycated with DX of MW ranging from 1 to 2,000 kDa, and the MW of purified glycates was determined using size-exclusion chromatography coupled with multiangle laser light scattering.					
29778476	12	29	theme	binding	1892:1898	arg1	ratios					1900:1905	The DX binding ratios	1885:1905	The DX binding ratios	1885:1905	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	13	30	theme	protein	2266:2272	arg1	surface					2251:2257	the surface	2247:2257	the surface of the protein	2247:2272	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	0	31	theme	molar	53:57	arg1	mass					59:62	different molar mass	43:62	different molar mass	43:62	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	0	32	theme	whey	13:16	arg1	protein					18:24	whey protein	13:24	whey protein	13:24	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	9	33	from	WPI	1421:1423	arg1	glycates					1451:1458	the glycates	1447:1458	the glycates made from DX that had MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa	1447:1535	The WPI to DX molar ratios in the glycates made from DX that had MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa were 1:4, 1:3, 1:2, 1:1.5, 1:1, and 1:1, respectively.					
29778476	6	34	theme	protein	955:961	arg1	isolate					963:969	whey protein isolate	950:969	whey protein isolate (WPI)	950:975	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	6	34	theme	protein	955:961	arg1	WPI					972:974	WPI	972:974	WPI	972:974	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	8	35	theme	size-exclusion	1339:1352	arg1	chromatography					1354:1367	size-exclusion chromatography	1339:1367	size-exclusion chromatography coupled with multiangle laser light scattering	1339:1414	The WPI was glycated with DX of MW ranging from 1 to 2,000 kDa, and the MW of purified glycates was determined using size-exclusion chromatography coupled with multiangle laser light scattering.					
29778476	10	36	theme	DX	1734:1735	arg1	number					1718:1723	the number	1714:1723	the number of bound DX	1714:1735	With the increase in the MW of DX, there was an increase in the MW values of the corresponding glycates but a decrease in the number of bound DX.					
29778476	7	37	theme	sera	1125:1128	arg1	sample					1130:1135	a composite sera sample	1113:1135	a composite sera sample	1113:1135	Blood sera were obtained from 8 patients who had been diagnosed with cow milk protein allergy, and a composite sera sample was used for IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method.					
29778476	5	38	theme	aqueous	857:863	arg1	solutions					865:873	aqueous solutions	857:873	aqueous solutions	857:873	Glycation was performed using the initial step of the Maillard reaction performed in aqueous solutions.					
29778476	6	39	theme	IgE-binding	926:936	arg1	capacity					938:945	the IgE-binding capacity	922:945	the IgE-binding capacity of whey protein isolate (WPI)	922:975	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	8	40	theme	multiangle	1382:1391	arg1	scattering					1405:1414	multiangle laser light scattering	1382:1414	multiangle laser light scattering	1382:1414	The WPI was glycated with DX of MW ranging from 1 to 2,000 kDa, and the MW of purified glycates was determined using size-exclusion chromatography coupled with multiangle laser light scattering.					
29778476	0	41	theme	E-binding	90:98	arg1	capacity					100:107	immunoglobulin E-binding capacity	75:107	immunoglobulin E-binding capacity	75:107	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	8	42	theme	light	1399:1403	arg1	scattering					1405:1414	multiangle laser light scattering	1382:1414	multiangle laser light scattering	1382:1414	The WPI was glycated with DX of MW ranging from 1 to 2,000 kDa, and the MW of purified glycates was determined using size-exclusion chromatography coupled with multiangle laser light scattering.					
29778476	4	43	theme	molar	699:703	arg1	mass					705:708	molar mass	699:708	molar mass; MW	699:712	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	4	43	theme	molar	699:703	arg1	size					693:696	the size	689:696	the size (molar mass; MW) of the polysaccharide	689:735	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	2	44	theme	IgE-binding	354:364	arg1	epitopes					366:373	IgE-binding epitopes	354:373	IgE-binding epitopes of the protein allergen	354:397	One potential approach to decrease protein allergenicity is to block IgE-binding epitopes of the protein allergen by attachment of polysaccharides via the Maillard reaction (i.e., glycation).					
29778476	7	45	theme	protein	1092:1098	arg1	allergy					1100:1106	cow milk protein allergy	1083:1106	cow milk protein allergy	1083:1106	Blood sera were obtained from 8 patients who had been diagnosed with cow milk protein allergy, and a composite sera sample was used for IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method.					
29778476	9	46	theme	molar	1431:1435	arg1	ratios					1437:1442	DX molar ratios	1428:1442	DX molar ratios	1428:1442	The WPI to DX molar ratios in the glycates made from DX that had MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa were 1:4, 1:3, 1:2, 1:1.5, 1:1, and 1:1, respectively.					
29778476	11	47	theme	lowest	1828:1833	arg1	capacity					1847:1854	the lowest IgE-binding capacity	1824:1854	the lowest IgE-binding capacity obtained in the G10 glycate	1824:1882	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	8	48	theme	MW	1254:1255	arg1	DX					1248:1249	DX	1248:1249	DX of MW ranging from 1 to 2,000 kDa	1248:1283	The WPI was glycated with DX of MW ranging from 1 to 2,000 kDa, and the MW of purified glycates was determined using size-exclusion chromatography coupled with multiangle laser light scattering.					
29778476	7	49	theme	cow	1083:1085	arg1	allergy					1100:1106	cow milk protein allergy	1083:1106	cow milk protein allergy	1083:1106	Blood sera were obtained from 8 patients who had been diagnosed with cow milk protein allergy, and a composite sera sample was used for IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method.					
29778476	13	50	from	surface	2251:2257	arg1	due					2188:2190	due	2188:2190	due	2188:2190	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	13	50	from	surface	2251:2257	arg1	binding					2150:2156	The lower IgE binding	2136:2156	The lower IgE binding of the G10 glycate	2136:2175	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	11	51	theme	G10	1872:1874	arg1	glycate					1876:1882	the G10 glycate	1868:1882	the G10 glycate	1868:1882	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	12	52	theme	molecules	2108:2116	arg1	attachment					2083:2092	the attachment	2079:2092	the attachment of up to 4 DX molecules per whey protein	2079:2133	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	2	53	theme	protein	382:388	arg1	allergen					390:397	the protein allergen	378:397	the protein allergen	378:397	One potential approach to decrease protein allergenicity is to block IgE-binding epitopes of the protein allergen by attachment of polysaccharides via the Maillard reaction (i.e., glycation).					
29778476	1	54	theme	protein	266:272	arg1	allergies					274:282	food protein allergies	261:282	food protein allergies	261:282	A growing concern around the world is the number of people who are suffering from food protein allergies.					
29778476	14	55	with	WPI	2326:2328	arg1	DX					2335:2336	DX	2335:2336	DX	2335:2336	In summary, our results demonstrate that glycating WPI with DX via Maillard reaction can potentially be used to decrease the allergenicity of whey protein.					
29778476	2	56	theme	Maillard	440:447	arg1	reaction					449:456	the Maillard reaction	436:456	the Maillard reaction (i.e., glycation)	436:474	One potential approach to decrease protein allergenicity is to block IgE-binding epitopes of the protein allergen by attachment of polysaccharides via the Maillard reaction (i.e., glycation).					
29778476	4	57	with	patients	631:638	arg1	allergy					662:668	cow milk protein allergy	645:668	cow milk protein allergy	645:668	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	14	58	theme	glycating	2316:2324	arg1	WPI					2326:2328	glycating WPI	2316:2328	glycating WPI with DX via Maillard reaction	2316:2358	In summary, our results demonstrate that glycating WPI with DX via Maillard reaction can potentially be used to decrease the allergenicity of whey protein.					
29778476	0	59	with	Glycation	0:8	arg1	dextrans					31:38	dextrans	31:38	dextrans of different molar mass	31:62	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	2	60	dep	glycation	465:473	arg1	i.e.					459:462	i.e.	459:462	i.e.	459:462	One potential approach to decrease protein allergenicity is to block IgE-binding epitopes of the protein allergen by attachment of polysaccharides via the Maillard reaction (i.e., glycation).					
29778476	4	61	theme	IgE-binding	750:760	arg1	capacity					762:769	this IgE-binding capacity	745:769	this IgE-binding capacity	745:769	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	6	62	theme	specific	880:887	arg1	goal					889:892	The specific goal	876:892	The specific goal of this study	876:906	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	9	63	theme	MW	1482:1483	arg1	values					1485:1490	MW values	1482:1490	MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa	1482:1535	The WPI to DX molar ratios in the glycates made from DX that had MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa were 1:4, 1:3, 1:2, 1:1.5, 1:1, and 1:1, respectively.					
29778476	0	64	theme	protein	162:168	arg1	allergy					170:176	cow milk protein allergy	153:176	cow milk protein allergy	153:176	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	11	65	theme	native	1807:1812	arg1	WPI					1814:1816	native WPI	1807:1816	native WPI	1807:1816	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	13	66	theme	lower	2140:2144	arg1	due					2188:2190	due	2188:2190	due	2188:2190	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	13	66	theme	lower	2140:2144	arg1	binding					2150:2156	The lower IgE binding	2136:2156	The lower IgE binding of the G10 glycate	2136:2175	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	12	67	theme	protein	2037:2043	arg1	coverage					2053:2060	extensive protein surface coverage	2027:2060	extensive protein surface coverage	2027:2060	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	1	68	theme	people	231:236	arg1	concern					189:195	A growing concern	179:195	A growing concern around the world	179:212	A growing concern around the world is the number of people who are suffering from food protein allergies.					
29778476	1	68	theme	people	231:236	arg1	number					221:226	the number	217:226	the number of people who are suffering from food protein allergies	217:282	A growing concern around the world is the number of people who are suffering from food protein allergies.					
29778476	10	69	from	increase	1601:1608	arg1	MW					1617:1618	the MW	1613:1618	the MW of DX	1613:1624	With the increase in the MW of DX, there was an increase in the MW values of the corresponding glycates but a decrease in the number of bound DX.					
29778476	12	70	with	glycation	1981:1989	arg1	DX					2012:2013	small-MW DX	2003:2013	small-MW DX	2003:2013	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	12	71	from	images	1959:1964	arg1	ratios					1900:1905	The DX binding ratios	1885:1905	The DX binding ratios	1885:1905	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	12	71	from	images	1959:1964	arg1	results					1922:1928	morphology results	1911:1928	morphology results	1911:1928	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	13	72	theme	glycate	2169:2175	arg1	due					2188:2190	due	2188:2190	due	2188:2190	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	13	72	theme	glycate	2169:2175	arg1	binding					2150:2156	The lower IgE binding	2136:2156	The lower IgE binding of the G10 glycate	2136:2175	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	3	73	theme	functional	542:551	arg1	properties					553:562	various functional properties	534:562	various functional properties	534:562	Protein glycation has been extensively studied to modify various functional properties.					
29778476	4	74	theme	cow	645:647	arg1	allergy					662:668	cow milk protein allergy	645:668	cow milk protein allergy	645:668	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	0	75	theme	blood	114:118	arg1	sera					120:123	blood sera	114:123	blood sera obtained from patients with cow milk protein allergy	114:176	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	0	76	from	Effect	65:70	arg1	capacity					100:107	immunoglobulin E-binding capacity	75:107	immunoglobulin E-binding capacity	75:107	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	6	77	theme	study	902:906	arg1	goal					889:892	The specific goal	876:892	The specific goal of this study	876:906	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	8	78	theme	purified	1300:1307	arg1	glycates					1309:1316	purified glycates	1300:1316	purified glycates	1300:1316	The WPI was glycated with DX of MW ranging from 1 to 2,000 kDa, and the MW of purified glycates was determined using size-exclusion chromatography coupled with multiangle laser light scattering.					
29778476	14	79	theme	whey	2417:2420	arg1	protein					2422:2428	whey protein	2417:2428	whey protein	2417:2428	In summary, our results demonstrate that glycating WPI with DX via Maillard reaction can potentially be used to decrease the allergenicity of whey protein.					
29778476	10	80	theme	glycates	1687:1694	arg1	values					1659:1664	the MW values	1652:1664	the MW values of the corresponding glycates	1652:1694	With the increase in the MW of DX, there was an increase in the MW values of the corresponding glycates but a decrease in the number of bound DX.					
29778476	10	81	from	increase	1640:1647	arg1	values					1659:1664	the MW values	1652:1664	the MW values of the corresponding glycates	1652:1694	With the increase in the MW of DX, there was an increase in the MW values of the corresponding glycates but a decrease in the number of bound DX.					
29778476	11	82	theme	protein	1773:1779	arg1	capacity					1793:1800	lower whey protein IgE-binding capacity	1762:1800	lower whey protein IgE-binding capacity	1762:1800	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	12	83	theme	WPI	1994:1996	arg1	glycation					1981:1989	glycation	1981:1989	glycation of WPI with small-MW DX	1981:2013	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	11	84	contain	had	1758:1760	arg1	glycates					1749:1756	The WPI-DX glycates	1738:1756	The WPI-DX glycates	1738:1756	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	11	84	contain	had	1758:1760	arg2	capacity					1793:1800	lower whey protein IgE-binding capacity	1762:1800	lower whey protein IgE-binding capacity	1762:1800	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	0	85	theme	cow	153:155	arg1	allergy					170:176	cow milk protein allergy	153:176	cow milk protein allergy	153:176	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	11	86	theme	lower	1762:1766	arg1	capacity					1793:1800	lower whey protein IgE-binding capacity	1762:1800	lower whey protein IgE-binding capacity	1762:1800	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	0	87	theme	mass	59:62	arg1	dextrans					31:38	dextrans	31:38	dextrans of different molar mass	31:62	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	5	88	theme	reaction	835:842	arg1	step					814:817	the initial step	802:817	the initial step of the Maillard reaction performed in aqueous solutions	802:873	Glycation was performed using the initial step of the Maillard reaction performed in aqueous solutions.					
29778476	6	89	theme	isolate	963:969	arg1	capacity					938:945	the IgE-binding capacity	922:945	the IgE-binding capacity of whey protein isolate (WPI)	922:975	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	4	90	theme	protein	654:660	arg1	allergy					662:668	cow milk protein allergy	645:668	cow milk protein allergy	645:668	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	7	91	theme	composite	1115:1123	arg1	sample					1130:1135	a composite sera sample	1113:1135	a composite sera sample	1113:1135	Blood sera were obtained from 8 patients who had been diagnosed with cow milk protein allergy, and a composite sera sample was used for IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method.					
29778476	12	92	theme	atomic	1935:1940	arg1	microscopy					1948:1957	atomic force microscopy	1935:1957	atomic force microscopy images	1935:1964	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	6	93	theme	whey	950:953	arg1	isolate					963:969	whey protein isolate	950:969	whey protein isolate (WPI)	950:975	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	6	93	theme	whey	950:953	arg1	WPI					972:974	WPI	972:974	WPI	972:974	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	0	94	with	patients	139:146	arg1	allergy					170:176	cow milk protein allergy	153:176	cow milk protein allergy	153:176	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	10	95	theme	bound	1728:1732	arg1	DX					1734:1735	bound DX	1728:1735	bound DX	1728:1735	With the increase in the MW of DX, there was an increase in the MW values of the corresponding glycates but a decrease in the number of bound DX.					
29778476	0	96	theme	immunoglobulin	75:88	arg1	capacity					100:107	immunoglobulin E-binding capacity	75:107	immunoglobulin E-binding capacity	75:107	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	4	97	theme	polysaccharide	722:735	arg1	mass					705:708	molar mass	699:708	molar mass; MW	699:712	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	4	97	theme	polysaccharide	722:735	arg1	size					693:696	the size	689:696	the size (molar mass; MW) of the polysaccharide	689:735	We wanted to examine whether glycates could reduce IgE binding in patients with cow milk protein allergy and to explore how the size (molar mass; MW) of the polysaccharide affects this IgE-binding capacity.					
29778476	8	98	theme	laser	1393:1397	arg1	scattering					1405:1414	multiangle laser light scattering	1382:1414	multiangle laser light scattering	1382:1414	The WPI was glycated with DX of MW ranging from 1 to 2,000 kDa, and the MW of purified glycates was determined using size-exclusion chromatography coupled with multiangle laser light scattering.					
29778476	12	99	theme	microscopy	1948:1957	arg1	images					1959:1964	atomic force microscopy images	1935:1964	atomic force microscopy images	1935:1964	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	12	100	theme	morphology	1911:1920	arg1	results					1922:1928	morphology results	1911:1928	morphology results	1911:1928	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	7	101	theme	milk	1087:1090	arg1	allergy					1100:1106	cow milk protein allergy	1083:1106	cow milk protein allergy	1083:1106	Blood sera were obtained from 8 patients who had been diagnosed with cow milk protein allergy, and a composite sera sample was used for IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method.					
29778476	12	102	dep	4	2103:2103	arg1	to					2100:2101	to	2100:2101	to	2100:2101	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	9	103	theme	DX	1428:1429	arg1	ratios					1437:1442	DX molar ratios	1428:1442	DX molar ratios	1428:1442	The WPI to DX molar ratios in the glycates made from DX that had MW values of 1, 3.5, 10 (G10), 150, 500, and 2,000 kDa were 1:4, 1:3, 1:2, 1:1.5, 1:1, and 1:1, respectively.					
29778476	2	104	theme	polysaccharides	416:430	arg1	attachment					402:411	attachment	402:411	attachment of polysaccharides	402:430	One potential approach to decrease protein allergenicity is to block IgE-binding epitopes of the protein allergen by attachment of polysaccharides via the Maillard reaction (i.e., glycation).					
29778476	12	105	theme	DX	2105:2106	arg1	molecules					2108:2116	up to 4 DX molecules	2097:2116	up to 4 DX molecules per whey protein	2097:2133	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	0	106	theme	protein	18:24	arg1	Glycation					0:8	Glycation	0:8	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.	0:177	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	10	107	from	decrease	1702:1709	arg1	number					1718:1723	the number	1714:1723	the number of bound DX	1714:1735	With the increase in the MW of DX, there was an increase in the MW values of the corresponding glycates but a decrease in the number of bound DX.					
29778476	14	108	theme	Maillard	2342:2349	arg1	reaction					2351:2358	Maillard reaction	2342:2358	Maillard reaction	2342:2358	In summary, our results demonstrate that glycating WPI with DX via Maillard reaction can potentially be used to decrease the allergenicity of whey protein.					
29778476	11	109	theme	IgE-binding	1835:1845	arg1	capacity					1847:1854	the lowest IgE-binding capacity	1824:1854	the lowest IgE-binding capacity obtained in the G10 glycate	1824:1882	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	12	110	theme	DX	1889:1890	arg1	ratios					1900:1905	The DX binding ratios	1885:1905	The DX binding ratios	1885:1905	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	2	111	theme	allergen	390:397	arg1	epitopes					366:373	IgE-binding epitopes	354:373	IgE-binding epitopes of the protein allergen	354:397	One potential approach to decrease protein allergenicity is to block IgE-binding epitopes of the protein allergen by attachment of polysaccharides via the Maillard reaction (i.e., glycation).					
29778476	13	112	theme	steric	2203:2208	arg1	barrier					2235:2241	a physical barrier	2224:2241	a physical barrier	2224:2241	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	13	112	theme	steric	2203:2208	arg1	hindrance					2210:2218	greater steric hindrance	2195:2218	greater steric hindrance (or a physical barrier)	2195:2242	The lower IgE binding of the G10 glycate was likely due to greater steric hindrance (or a physical barrier) at the surface of the protein.					
29778476	1	113	theme	food	261:264	arg1	allergies					274:282	food protein allergies	261:282	food protein allergies	261:282	A growing concern around the world is the number of people who are suffering from food protein allergies.					
29778476	0	114	theme	different	43:51	arg1	mass					59:62	different molar mass	43:62	different molar mass	43:62	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	11	115	theme	IgE-binding	1781:1791	arg1	capacity					1793:1800	lower whey protein IgE-binding capacity	1762:1800	lower whey protein IgE-binding capacity	1762:1800	The WPI-DX glycates had lower whey protein IgE-binding capacity than native WPI, with the lowest IgE-binding capacity obtained in the G10 glycate.					
29778476	7	116	theme	ImmunoCap	1178:1186	arg1	method					1214:1219	the ImmunoCap (Phadia, Uppsala, Sweden) method	1174:1219	the ImmunoCap (Phadia, Uppsala, Sweden) method	1174:1219	Blood sera were obtained from 8 patients who had been diagnosed with cow milk protein allergy, and a composite sera sample was used for IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method.					
29778476	3	117	theme	Protein	477:483	arg1	glycation					485:493	Protein glycation	477:493	Protein glycation	477:493	Protein glycation has been extensively studied to modify various functional properties.					
29778476	12	118	theme	extensive	2027:2035	arg1	coverage					2053:2060	extensive protein surface coverage	2027:2060	extensive protein surface coverage	2027:2060	The DX binding ratios and morphology results from atomic force microscopy images suggested that glycation of WPI with small-MW DX resulted in extensive protein surface coverage, probably due to the attachment of up to 4 DX molecules per whey protein.					
29778476	0	119	dep	Glycation	0:8	arg1	Effect					65:70	Effect	65:70	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.	0:177	Glycation of whey protein with dextrans of different molar mass: Effect on immunoglobulin E-binding capacity with blood sera obtained from patients with cow milk protein allergy.					
29778476	7	120	used	used	1141:1144	arg2	sample					1130:1135	a composite sera sample	1113:1135	a composite sera sample	1113:1135	Blood sera were obtained from 8 patients who had been diagnosed with cow milk protein allergy, and a composite sera sample was used for IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method.					
29778476	2	121	theme	potential	289:297	arg1	approach					299:306	One potential approach	285:306	One potential approach to decrease protein allergenicity	285:340	One potential approach to decrease protein allergenicity is to block IgE-binding epitopes of the protein allergen by attachment of polysaccharides via the Maillard reaction (i.e., glycation).					
29778476	6	122	with	glycation	985:993	arg1	DX					1009:1010	DX	1009:1010	DX	1009:1010	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	6	122	with	glycation	985:993	arg1	dextran					1000:1006	dextran	1000:1006	dextran (DX)	1000:1011	The specific goal of this study was to reduce the IgE-binding capacity of whey protein isolate (WPI) through glycation with dextran (DX).					
29778476	7	123	theme	IgE-binding	1150:1160	arg1	analysis					1162:1169	IgE-binding analysis	1150:1169	IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method	1150:1219	Blood sera were obtained from 8 patients who had been diagnosed with cow milk protein allergy, and a composite sera sample was used for IgE-binding analysis by the ImmunoCap (Phadia, Uppsala, Sweden) method.					
31785876	11	0	theme	DH	1872:1873	arg1	value					1875:1879	a DH value	1870:1879	a DH value of 27.1%	1870:1888	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	0	1	theme	milk	162:165	arg1	allergy					175:181	a cow milk protein allergy	156:181	a cow milk protein allergy	156:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	3	2	theme	enzymatic	515:523	arg1	hydrolysis					525:534	enzymatic hydrolysis	515:534	enzymatic hydrolysis	515:534	We wanted to examine whether enzymatic hydrolysis, combined with glycation, could further reduce IgE binding for people with a cow milk protein allergy.					
31785876	4	3	theme	%	768:768	arg1	values					750:755	hydrolysis (DH) values	734:755	hydrolysis (DH) values of 17 to 27%	734:768	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	2	4	theme	proteins	254:261	arg1	Hydrolysis					235:244	Hydrolysis	235:244	Hydrolysis of food proteins	235:261	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	6	5	theme	aqueous	1045:1051	arg1	solutions					1053:1061	aqueous solutions	1045:1061	aqueous solutions held at 62°C for 24 h	1045:1083	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	5	6	used	used	796:799	arg2	enzymes					783:789	Immobilized enzymes	771:789	Immobilized enzymes	771:789	Immobilized enzymes were used to avoid heat-treating the hydrolysate (to inactivate the enzymes, because heating could also affect the IgE binding ability of the protein).					
31785876	3	7	with	people	599:604	arg1	allergy					630:636	a cow milk protein allergy	611:636	a cow milk protein allergy	611:636	We wanted to examine whether enzymatic hydrolysis, combined with glycation, could further reduce IgE binding for people with a cow milk protein allergy.					
31785876	9	8	theme	DH	1512:1513	arg1	values					1515:1520	DH values	1512:1520	DH values of 18.7, 22.5, and 27.1%	1512:1545	The average MW values of samples WPIH-1 to WPIH-3 decreased from 11.15, 9.46, and 7.57 kDa with increasing DH values of 18.7, 22.5, and 27.1%.					
31785876	0	9	theme	blood	117:121	arg1	sera					123:126	blood sera	117:126	blood sera obtained from patients with a cow milk protein allergy	117:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	8	10	from	patients	1284:1291	arg1	sera					1272:1275	blood sera	1266:1275	blood sera from 8 patients who had been diagnosed with a cow milk protein allergy	1266:1346	We obtained blood sera from 8 patients who had been diagnosed with a cow milk protein allergy, and we used a composite serum for IgE binding analysis.					
31785876	1	11	theme	protein	189:195	arg1	concern					226:232	a major global concern	211:232	a major global concern	211:232	Food protein allergies are a major global concern.					
31785876	1	11	theme	protein	189:195	arg1	allergies					197:205	Food protein allergies	184:205	Food protein allergies	184:205	Food protein allergies are a major global concern.					
31785876	2	12	from	epitopes	452:459	arg1	allergen					476:483	the protein allergen	464:483	the protein allergen	464:483	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	5	13	theme	Immobilized	771:781	arg1	enzymes					783:789	Immobilized enzymes	771:789	Immobilized enzymes	771:789	Immobilized enzymes were used to avoid heat-treating the hydrolysate (to inactivate the enzymes, because heating could also affect the IgE binding ability of the protein).					
31785876	7	14	theme	light	1236:1240	arg1	scattering					1242:1251	multi-angle laser light scattering	1218:1251	multi-angle laser light scattering	1218:1251	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	11	15	dep	reduction	1801:1809	arg1	%					1799:1799	%	1799:1799	%	1799:1799	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	8	16	theme	cow	1323:1325	arg1	allergy					1340:1346	a cow milk protein allergy	1321:1346	a cow milk protein allergy	1321:1346	We obtained blood sera from 8 patients who had been diagnosed with a cow milk protein allergy, and we used a composite serum for IgE binding analysis.					
31785876	7	17	theme	multi-angle	1218:1228	arg1	scattering					1242:1251	multi-angle laser light scattering	1218:1251	multi-angle laser light scattering	1218:1251	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	2	18	theme	protein	468:474	arg1	allergen					476:483	the protein allergen	464:483	the protein allergen	464:483	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	8	19	theme	protein	1332:1338	arg1	allergy					1340:1346	a cow milk protein allergy	1321:1346	a cow milk protein allergy	1321:1346	We obtained blood sera from 8 patients who had been diagnosed with a cow milk protein allergy, and we used a composite serum for IgE binding analysis.					
31785876	7	20	theme	glycates	1158:1165	arg1	MW					1114:1115	MW	1114:1115	MW	1114:1115	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	7	20	theme	glycates	1158:1165	arg1	mass					1108:1111	the molar mass	1098:1111	the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX)	1098:1175	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	4	21	theme	protein	644:650	arg1	isolate					652:658	Whey protein isolate	639:658	Whey protein isolate (WPI)	639:664	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	4	21	theme	protein	644:650	arg1	WPI					661:663	WPI	661:663	WPI	661:663	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	7	22	theme	molar	1102:1106	arg1	MW					1114:1115	MW	1114:1115	MW	1114:1115	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	7	22	theme	molar	1102:1106	arg1	mass					1108:1111	the molar mass	1098:1111	the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX)	1098:1175	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	10	23	theme	samples	1612:1618	arg1	bitterness					1589:1598	the high bitterness	1580:1598	the high bitterness of the WPIH samples	1580:1618	Glycation significantly reduced the high bitterness of the WPIH samples, as assessed by a trained sensory panel.					
31785876	5	24	theme	heat-treating	810:822	arg1	hydrolysate					828:838	heat-treating the hydrolysate	810:838	heat-treating the hydrolysate	810:838	Immobilized enzymes were used to avoid heat-treating the hydrolysate (to inactivate the enzymes, because heating could also affect the IgE binding ability of the protein).					
31785876	0	25	from	Influence	0:8	arg1	combination					40:50	combination	40:50	combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy	40:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	0	26	with	patients	142:149	arg1	allergy					175:181	a cow milk protein allergy	156:181	a cow milk protein allergy	156:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	0	27	theme	E	93:93	arg1	capacity					103:110	immunoglobulin E binding capacity	78:110	immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy	78:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	2	28	theme	Maillard	385:392	arg1	reaction					394:401	the Maillard reaction	381:401	the Maillard reaction (i.e., glycation)	381:419	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	2	29	dep	glycation	410:418	arg1	i.e.					404:407	i.e.	404:407	i.e.	404:407	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	7	30	theme	size-exclusion	1183:1196	arg1	chromatography					1198:1211	size-exclusion chromatography	1183:1211	size-exclusion chromatography with multi-angle laser light scattering	1183:1251	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	12	31	theme	proteins	2015:2022	arg1	allergenicity					1993:2005	the allergenicity	1989:2005	the allergenicity of whey proteins	1989:2022	Hydrolysis of WPI followed by glycation with DX via the Maillard reaction significantly decreased the allergenicity of whey proteins.					
31785876	4	32	theme	immobilized	684:694	arg1	trypsin					696:702	immobilized trypsin	684:702	immobilized trypsin	684:702	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	9	33	theme	average	1409:1415	arg1	values					1420:1425	The average MW values	1405:1425	The average MW values of samples WPIH-1 to WPIH-3	1405:1453	The average MW values of samples WPIH-1 to WPIH-3 decreased from 11.15, 9.46, and 7.57 kDa with increasing DH values of 18.7, 22.5, and 27.1%.					
31785876	6	34	theme	isolate	970:976	arg1	hydrolysates					978:989	The resultant whey protein isolate hydrolysates	943:989	The resultant whey protein isolate hydrolysates (WPIH)	943:996	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	6	34	theme	isolate	970:976	arg1	WPIH					992:995	WPIH	992:995	WPIH	992:995	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	8	35	theme	IgE	1383:1385	arg1	analysis					1395:1402	IgE binding analysis	1383:1402	IgE binding analysis	1383:1402	We obtained blood sera from 8 patients who had been diagnosed with a cow milk protein allergy, and we used a composite serum for IgE binding analysis.					
31785876	11	36	theme	binding	1725:1731	arg1	capacity					1733:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	4	37	dep	27	766:767	arg1	to					763:764	to	763:764	to	763:764	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	6	38	theme	whey	957:960	arg1	hydrolysates					978:989	The resultant whey protein isolate hydrolysates	943:989	The resultant whey protein isolate hydrolysates (WPIH)	943:996	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	6	38	theme	whey	957:960	arg1	WPIH					992:995	WPIH	992:995	WPIH	992:995	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	2	39	theme	binding	444:450	arg1	epitopes					452:459	some IgE binding epitopes	435:459	some IgE binding epitopes on the protein allergen	435:483	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	3	40	theme	cow	613:615	arg1	allergy					630:636	a cow milk protein allergy	611:636	a cow milk protein allergy	611:636	We wanted to examine whether enzymatic hydrolysis, combined with glycation, could further reduce IgE binding for people with a cow milk protein allergy.					
31785876	8	41	theme	blood	1266:1270	arg1	sera					1272:1275	blood sera	1266:1275	blood sera from 8 patients who had been diagnosed with a cow milk protein allergy	1266:1346	We obtained blood sera from 8 patients who had been diagnosed with a cow milk protein allergy, and we used a composite serum for IgE binding analysis.					
31785876	11	42	theme	WPI-specific	1708:1719	arg1	capacity					1733:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	0	43	theme	whey	13:16	arg1	hydrolysis					26:35	whey protein hydrolysis	13:35	whey protein hydrolysis	13:35	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	0	44	theme	hydrolysis	26:35	arg1	Influence					0:8	Influence	0:8	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.	0:182	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	9	45	theme	samples	1430:1436	arg1	values					1420:1425	The average MW values	1405:1425	The average MW values of samples WPIH-1 to WPIH-3	1405:1453	The average MW values of samples WPIH-1 to WPIH-3 decreased from 11.15, 9.46, and 7.57 kDa with increasing DH values of 18.7, 22.5, and 27.1%.					
31785876	7	46	theme	corresponding	1144:1156	arg1	glycates					1158:1165	their corresponding glycates	1138:1165	their corresponding glycates (WPIH-DX)	1138:1175	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	7	46	theme	corresponding	1144:1156	arg1	WPIH-DX					1168:1174	WPIH-DX	1168:1174	WPIH-DX	1168:1174	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	0	47	theme	dextran	57:63	arg1	glycation					65:73	dextran glycation	57:73	dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy	57:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	2	48	theme	covalent	326:333	arg1	approach					310:317	another novel approach	296:317	another novel approach	296:317	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	2	48	theme	covalent	326:333	arg1	attachment					335:344	the covalent attachment	322:344	the covalent attachment of polysaccharides to proteins	322:375	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	3	49	theme	IgE	583:585	arg1	binding					587:593	IgE binding	583:593	IgE binding	583:593	We wanted to examine whether enzymatic hydrolysis, combined with glycation, could further reduce IgE binding for people with a cow milk protein allergy.					
31785876	5	50	theme	protein	933:939	arg1	ability					918:924	the IgE binding ability	902:924	the IgE binding ability of the protein	902:939	Immobilized enzymes were used to avoid heat-treating the hydrolysate (to inactivate the enzymes, because heating could also affect the IgE binding ability of the protein).					
31785876	5	51	theme	binding	910:916	arg1	ability					918:924	the IgE binding ability	902:924	the IgE binding ability of the protein	902:939	Immobilized enzymes were used to avoid heat-treating the hydrolysate (to inactivate the enzymes, because heating could also affect the IgE binding ability of the protein).					
31785876	7	52	theme	WPIH	1121:1124	arg1	samples					1126:1132	WPIH samples	1121:1132	WPIH samples	1121:1132	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	3	53	theme	protein	622:628	arg1	allergy					630:636	a cow milk protein allergy	611:636	a cow milk protein allergy	611:636	We wanted to examine whether enzymatic hydrolysis, combined with glycation, could further reduce IgE binding for people with a cow milk protein allergy.					
31785876	0	54	with	combination	40:50	arg1	glycation					65:73	dextran glycation	57:73	dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy	57:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	1	55	theme	global	219:224	arg1	concern					226:232	a major global concern	211:232	a major global concern	211:232	Food protein allergies are a major global concern.					
31785876	1	55	theme	global	219:224	arg1	allergies					197:205	Food protein allergies	184:205	Food protein allergies	184:205	Food protein allergies are a major global concern.					
31785876	0	56	theme	protein	167:173	arg1	allergy					175:181	a cow milk protein allergy	156:181	a cow milk protein allergy	156:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	2	57	attach	attachment	335:344	arg1	proteins					368:375	proteins	368:375	proteins	368:375	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	2	57	attach	attachment	335:344	arg2	polysaccharides					349:363	polysaccharides	349:363	polysaccharides	349:363	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	11	58	theme	%	1888:1888	arg1	value					1875:1879	a DH value	1870:1879	a DH value of 27.1%	1870:1888	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	10	59	theme	high	1584:1587	arg1	bitterness					1589:1598	the high bitterness	1580:1598	the high bitterness of the WPIH samples	1580:1618	Glycation significantly reduced the high bitterness of the WPIH samples, as assessed by a trained sensory panel.					
31785876	0	60	with	capacity	103:110	arg1	sera					123:126	blood sera	117:126	blood sera obtained from patients with a cow milk protein allergy	117:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	2	61	theme	novel	304:308	arg1	approach					310:317	another novel approach	296:317	another novel approach	296:317	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	2	61	theme	novel	304:308	arg1	attachment					335:344	the covalent attachment	322:344	the covalent attachment of polysaccharides to proteins	322:375	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	1	62	theme	Food	184:187	arg1	concern					226:232	a major global concern	211:232	a major global concern	211:232	Food protein allergies are a major global concern.					
31785876	1	62	theme	Food	184:187	arg1	allergies					197:205	Food protein allergies	184:205	Food protein allergies	184:205	Food protein allergies are a major global concern.					
31785876	11	63	theme	IgE	1814:1816	arg1	binding					1818:1824	IgE binding	1814:1824	IgE binding	1814:1824	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	11	64	theme	WPIH-DX	1834:1840	arg1	glycate					1842:1848	the WPIH-DX glycate	1830:1848	the WPIH-DX glycate made from WPIH with a DH value of 27.1%	1830:1888	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	10	65	theme	WPIH	1607:1610	arg1	samples					1612:1618	the WPIH samples	1603:1618	the WPIH samples	1603:1618	Glycation significantly reduced the high bitterness of the WPIH samples, as assessed by a trained sensory panel.					
31785876	0	66	from	glycation	65:73	arg1	capacity					103:110	immunoglobulin E binding capacity	78:110	immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy	78:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	10	67	theme	trained	1638:1644	arg1	panel					1654:1658	a trained sensory panel	1636:1658	a trained sensory panel	1636:1658	Glycation significantly reduced the high bitterness of the WPIH samples, as assessed by a trained sensory panel.					
31785876	8	68	theme	milk	1327:1330	arg1	allergy					1340:1346	a cow milk protein allergy	1321:1346	a cow milk protein allergy	1321:1346	We obtained blood sera from 8 patients who had been diagnosed with a cow milk protein allergy, and we used a composite serum for IgE binding analysis.					
31785876	0	69	theme	cow	158:160	arg1	allergy					175:181	a cow milk protein allergy	156:181	a cow milk protein allergy	156:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	2	70	theme	food	249:252	arg1	proteins					254:261	food proteins	249:261	food proteins	249:261	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	7	71	theme	laser	1230:1234	arg1	scattering					1242:1251	multi-angle laser light scattering	1218:1251	multi-angle laser light scattering	1218:1251	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	8	72	used	used	1356:1359	arg2	we					1353:1354	we	1353:1354	we	1353:1354	We obtained blood sera from 8 patients who had been diagnosed with a cow milk protein allergy, and we used a composite serum for IgE binding analysis.					
31785876	7	73	with	chromatography	1198:1211	arg1	scattering					1242:1251	multi-angle laser light scattering	1218:1251	multi-angle laser light scattering	1218:1251	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	11	74	theme	unglycated	1761:1770	arg1	WPIH					1772:1775	unglycated WPIH	1761:1775	unglycated WPIH	1761:1775	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	11	75	dep	%	1799:1799	arg1	99					1797:1798	99	1797:1798	99	1797:1798	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	9	76	theme	%	1545:1545	arg1	values					1515:1520	DH values	1512:1520	DH values of 18.7, 22.5, and 27.1%	1512:1545	The average MW values of samples WPIH-1 to WPIH-3 decreased from 11.15, 9.46, and 7.57 kDa with increasing DH values of 18.7, 22.5, and 27.1%.					
31785876	4	77	theme	Whey	639:642	arg1	isolate					652:658	Whey protein isolate	639:658	Whey protein isolate (WPI)	639:664	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	4	77	theme	Whey	639:642	arg1	WPI					661:663	WPI	661:663	WPI	661:663	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	0	78	theme	immunoglobulin	78:91	arg1	capacity					103:110	immunoglobulin E binding capacity	78:110	immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy	78:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	12	79	theme	whey	2010:2013	arg1	proteins					2015:2022	whey proteins	2010:2022	whey proteins	2010:2022	Hydrolysis of WPI followed by glycation with DX via the Maillard reaction significantly decreased the allergenicity of whey proteins.					
31785876	0	80	theme	binding	95:101	arg1	capacity					103:110	immunoglobulin E binding capacity	78:110	immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy	78:181	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	8	81	theme	composite	1363:1371	arg1	serum					1373:1377	a composite serum	1361:1377	a composite serum for IgE binding analysis	1361:1402	We obtained blood sera from 8 patients who had been diagnosed with a cow milk protein allergy, and we used a composite serum for IgE binding analysis.					
31785876	11	82	contain	had	1682:1684	arg2	capacity					1733:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	11	82	contain	had	1682:1684	arg1	glycates					1673:1680	The WPIH-DX glycates	1661:1680	The WPIH-DX glycates	1661:1680	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	12	83	with	glycation	1921:1929	arg1	DX					1936:1937	DX	1936:1937	DX	1936:1937	Hydrolysis of WPI followed by glycation with DX via the Maillard reaction significantly decreased the allergenicity of whey proteins.					
31785876	11	84	theme	reduced	1700:1706	arg1	capacity					1733:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	8	85	theme	binding	1387:1393	arg1	analysis					1395:1402	IgE binding analysis	1383:1402	IgE binding analysis	1383:1402	We obtained blood sera from 8 patients who had been diagnosed with a cow milk protein allergy, and we used a composite serum for IgE binding analysis.					
31785876	0	86	theme	protein	18:24	arg1	hydrolysis					26:35	whey protein hydrolysis	13:35	whey protein hydrolysis	13:35	Influence of whey protein hydrolysis in combination with dextran glycation on immunoglobulin E binding capacity with blood sera obtained from patients with a cow milk protein allergy.					
31785876	9	87	theme	MW	1417:1418	arg1	values					1420:1425	The average MW values	1405:1425	The average MW values of samples WPIH-1 to WPIH-3	1405:1453	The average MW values of samples WPIH-1 to WPIH-3 decreased from 11.15, 9.46, and 7.57 kDa with increasing DH values of 18.7, 22.5, and 27.1%.					
31785876	6	88	theme	protein	962:968	arg1	hydrolysates					978:989	The resultant whey protein isolate hydrolysates	943:989	The resultant whey protein isolate hydrolysates (WPIH)	943:996	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	6	88	theme	protein	962:968	arg1	WPIH					992:995	WPIH	992:995	WPIH	992:995	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	10	89	theme	sensory	1646:1652	arg1	panel					1654:1658	a trained sensory panel	1636:1658	a trained sensory panel	1636:1658	Glycation significantly reduced the high bitterness of the WPIH samples, as assessed by a trained sensory panel.					
31785876	12	90	theme	Maillard	1947:1954	arg1	reaction					1956:1963	the Maillard reaction	1943:1963	the Maillard reaction	1943:1963	Hydrolysis of WPI followed by glycation with DX via the Maillard reaction significantly decreased the allergenicity of whey proteins.					
31785876	6	91	theme	resultant	947:955	arg1	hydrolysates					978:989	The resultant whey protein isolate hydrolysates	943:989	The resultant whey protein isolate hydrolysates (WPIH)	943:996	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	6	91	theme	resultant	947:955	arg1	WPIH					992:995	WPIH	992:995	WPIH	992:995	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	2	92	theme	IgE	440:442	arg1	epitopes					452:459	some IgE binding epitopes	435:459	some IgE binding epitopes on the protein allergen	435:483	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	4	93	theme	values	750:755	arg1	degree					724:729	degree	724:729	degree of hydrolysis (DH) values of 17 to 27%	724:768	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	11	94	theme	IgE	1721:1723	arg1	capacity					1733:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	significantly reduced WPI-specific IgE binding capacity	1686:1740	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	9	95	theme	WPIH-1	1438:1443	arg1	samples					1430:1436	samples	1430:1436	samples WPIH-1 to WPIH-3	1430:1453	The average MW values of samples WPIH-1 to WPIH-3 decreased from 11.15, 9.46, and 7.57 kDa with increasing DH values of 18.7, 22.5, and 27.1%.					
31785876	7	96	theme	samples	1126:1132	arg1	MW					1114:1115	MW	1114:1115	MW	1114:1115	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	7	96	theme	samples	1126:1132	arg1	mass					1108:1111	the molar mass	1098:1111	the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX)	1098:1175	We analyzed the molar mass (MW) of WPIH samples and their corresponding glycates (WPIH-DX) using size-exclusion chromatography with multi-angle laser light scattering.					
31785876	12	97	theme	WPI	1905:1907	arg1	Hydrolysis					1891:1900	Hydrolysis	1891:1900	Hydrolysis of WPI followed by glycation with DX via the Maillard reaction	1891:1963	Hydrolysis of WPI followed by glycation with DX via the Maillard reaction significantly decreased the allergenicity of whey proteins.					
31785876	5	98	theme	IgE	906:908	arg1	ability					918:924	the IgE binding ability	902:924	the IgE binding ability of the protein	902:939	Immobilized enzymes were used to avoid heat-treating the hydrolysate (to inactivate the enzymes, because heating could also affect the IgE binding ability of the protein).					
31785876	6	99	theme	10-kDa	1022:1027	arg1	DX					1038:1039	DX	1038:1039	DX	1038:1039	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	6	99	theme	10-kDa	1022:1027	arg1	dextran					1029:1035	10-kDa dextran	1022:1035	10-kDa dextran (DX)	1022:1040	The resultant whey protein isolate hydrolysates (WPIH) were then glycated with 10-kDa dextran (DX) in aqueous solutions held at 62°C for 24 h.					
31785876	11	100	from	reduction	1801:1809	arg1	binding					1818:1824	IgE binding	1814:1824	IgE binding	1814:1824	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	3	101	theme	milk	617:620	arg1	allergy					630:636	a cow milk protein allergy	611:636	a cow milk protein allergy	611:636	We wanted to examine whether enzymatic hydrolysis, combined with glycation, could further reduce IgE binding for people with a cow milk protein allergy.					
31785876	4	102	theme	hydrolysis	734:743	arg1	values					750:755	hydrolysis (DH) values	734:755	hydrolysis (DH) values of 17 to 27%	734:768	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	11	103	theme	WPIH-DX	1665:1671	arg1	glycates					1673:1680	The WPIH-DX glycates	1661:1680	The WPIH-DX glycates	1661:1680	The WPIH-DX glycates had significantly reduced WPI-specific IgE binding capacity compared to WPI or unglycated WPIH; we found an almost 99% reduction in IgE binding for the WPIH-DX glycate made from WPIH with a DH value of 27.1%.					
31785876	1	104	theme	major	213:217	arg1	concern					226:232	a major global concern	211:232	a major global concern	211:232	Food protein allergies are a major global concern.					
31785876	1	104	theme	major	213:217	arg1	allergies					197:205	Food protein allergies	184:205	Food protein allergies	184:205	Food protein allergies are a major global concern.					
31785876	4	105	theme	DH	746:747	arg1	values					750:755	hydrolysis (DH) values	734:755	hydrolysis (DH) values of 17 to 27%	734:768	Whey protein isolate (WPI) was hydrolyzed by immobilized trypsin and chymotrypsin to degree of hydrolysis (DH) values of 17 to 27%.					
31785876	2	106	theme	polysaccharides	349:363	arg1	approach					310:317	another novel approach	296:317	another novel approach	296:317	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
31785876	2	106	theme	polysaccharides	349:363	arg1	attachment					335:344	the covalent attachment	322:344	the covalent attachment of polysaccharides to proteins	322:375	Hydrolysis of food proteins reduces their allergenicity, but another novel approach is the covalent attachment of polysaccharides to proteins via the Maillard reaction (i.e., glycation), which blocks some IgE binding epitopes on the protein allergen.					
29938358	7	0	theme	narrow	1217:1222	arg1	layer					1224:1228	broad and narrow layer	1207:1228	broad and narrow layer	1207:1228	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	8	1	theme	plants	1508:1513	arg1	utilization					1487:1497	utilization	1487:1497	utilization of woody plants	1487:1513	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	7	2	theme	outermost	1114:1122	arg1	fiber					1131:1135	the outermost phloem fiber	1110:1135	the outermost phloem fiber near the periderm	1110:1153	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	6	3	theme	comparative	857:867	arg1	analysis					869:876	The comparative analysis	853:876	The comparative analysis of cell wall composition in phloem fiber	853:917	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	4	4	theme	xylem	467:471	arg1	fiber					473:477	xylem fiber	467:477	xylem fiber	467:477	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	6	5	theme	phloem	906:911	arg1	fiber					913:917	phloem fiber	906:917	phloem fiber	906:917	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	7	6	theme	phloem	1124:1129	arg1	fiber					1131:1135	the outermost phloem fiber	1110:1135	the outermost phloem fiber near the periderm	1110:1153	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	7	7	theme	broad	1239:1243	arg1	lamellae					1245:1252	the broad lamellae	1235:1252	the broad lamellae	1235:1252	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	4	8	theme	cell	570:573	arg1	corner					575:580	cell corner	570:580	cell corner	570:580	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	3	9	theme	cell	358:361	arg1	deposition					368:377	earlier cell wall deposition	350:377	earlier cell wall deposition in vessel and afterwards in the neighboring fiber	350:427	During xylem formation, earlier cell wall deposition in vessel and afterwards in the neighboring fiber was observed in situ.					
29938358	4	10	theme	wall	500:503	arg1	process					531:537	an ordered process	520:537	an ordered process which lignification started in cell corner following carbohydrates deposition	520:615	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	4	10	theme	wall	500:503	arg1	deposition					505:514	cell wall deposition	495:514	cell wall deposition	495:514	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	6	11	theme	cell	881:884	arg1	composition					891:901	cell wall composition	881:901	cell wall composition in phloem fiber	881:917	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	4	12	theme	carbohydrates	592:604	arg1	deposition					606:615	carbohydrates deposition	592:615	carbohydrates deposition	592:615	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	3	13	theme	wall	363:366	arg1	deposition					368:377	earlier cell wall deposition	350:377	earlier cell wall deposition in vessel and afterwards in the neighboring fiber	350:427	During xylem formation, earlier cell wall deposition in vessel and afterwards in the neighboring fiber was observed in situ.					
29938358	3	14	from	deposition	368:377	arg1	vessel					382:387	vessel	382:387	vessel	382:387	During xylem formation, earlier cell wall deposition in vessel and afterwards in the neighboring fiber was observed in situ.					
29938358	3	14	from	deposition	368:377	arg1	fiber					423:427	the neighboring fiber	407:427	the neighboring fiber	407:427	During xylem formation, earlier cell wall deposition in vessel and afterwards in the neighboring fiber was observed in situ.					
29938358	8	15	theme	cell	1351:1354	arg1	composition					1361:1371	cell wall composition	1351:1371	cell wall composition	1351:1371	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	5	16	theme	Higher	618:623	arg1	deposition					625:634	Higher deposition	618:634	Higher deposition speed of carbohydrates	618:657	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	5	17	theme	units	744:748	arg1	deposition					750:759	the syringyl (S) units deposition	727:759	the syringyl (S) units deposition	727:759	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	2	18	from	variations	202:211	arg1	xylem					229:233	xylem	229:233	xylem	229:233	The topochemical variations in newly formed xylem and phloem of Populus × euramericana were investigated by combined microscopic techniques.					
29938358	2	18	from	variations	202:211	arg1	phloem					239:244	phloem	239:244	phloem	239:244	The topochemical variations in newly formed xylem and phloem of Populus × euramericana were investigated by combined microscopic techniques.					
29938358	6	19	from	composition	891:901	arg1	fiber					913:917	phloem fiber	906:917	phloem fiber	906:917	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	5	20	theme	S	741:741	arg1	units					744:748	the syringyl (S) units	727:748	the syringyl (S) units deposition	727:759	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	6	21	theme	fiber	1040:1044	arg1	location					1046:1053	phloem fiber location	1033:1053	phloem fiber location	1033:1053	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	2	22	theme	Populus × euramericana	249:270	arg1	xylem					229:233	xylem	229:233	xylem	229:233	The topochemical variations in newly formed xylem and phloem of Populus × euramericana were investigated by combined microscopic techniques.					
29938358	2	22	theme	Populus × euramericana	249:270	arg1	phloem					239:244	phloem	239:244	phloem	239:244	The topochemical variations in newly formed xylem and phloem of Populus × euramericana were investigated by combined microscopic techniques.					
29938358	1	23	theme	whole	141:145	arg1	process					161:167	the whole lignification process	137:167	the whole lignification process of xylem fiber	137:182	Both G units and S units deposited in the whole lignification process of xylem fiber.					
29938358	3	24	theme	earlier	350:356	arg1	deposition					368:377	earlier cell wall deposition	350:377	earlier cell wall deposition in vessel and afterwards in the neighboring fiber	350:427	During xylem formation, earlier cell wall deposition in vessel and afterwards in the neighboring fiber was observed in situ.					
29938358	7	25	theme	broad	1207:1211	arg1	layer					1224:1228	broad and narrow layer	1207:1228	broad and narrow layer	1207:1228	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	1	26	theme	lignification	147:159	arg1	process					161:167	the whole lignification process	137:167	the whole lignification process of xylem fiber	137:182	Both G units and S units deposited in the whole lignification process of xylem fiber.					
29938358	7	27	theme	multilayered	1167:1178	arg1	structure					1180:1188	a multilayered structure	1165:1188	a multilayered structure	1165:1188	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	5	28	theme	differentiation	706:720	arg1	beginning					679:687	the beginning	675:687	the beginning of the cell wall differentiation	675:720	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	7	29	theme	interesting	1072:1082	arg1	phenomenon					1084:1093	an interesting phenomenon	1069:1093	an interesting phenomenon	1069:1093	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	3	30	located	observed	433:440	arg2	deposition					368:377	earlier cell wall deposition	350:377	earlier cell wall deposition in vessel and afterwards in the neighboring fiber	350:427	During xylem formation, earlier cell wall deposition in vessel and afterwards in the neighboring fiber was observed in situ.					
29938358	3	30	located	observed	433:440	arg1	situ					445:448	situ	445:448	situ	445:448	During xylem formation, earlier cell wall deposition in vessel and afterwards in the neighboring fiber was observed in situ.					
29938358	6	31	from	fiber	913:917	arg1	analysis					869:876	The comparative analysis	853:876	The comparative analysis of cell wall composition in phloem fiber	853:917	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	5	32	theme	G	805:805	arg1	units					808:812	guaiacyl (G) units	795:812	guaiacyl (G) units	795:812	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	6	33	from	analysis	869:876	arg1	fiber					913:917	phloem fiber	906:917	phloem fiber	906:917	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	2	34	theme	microscopic	302:312	arg1	techniques					314:323	combined microscopic techniques	293:323	combined microscopic techniques	293:323	The topochemical variations in newly formed xylem and phloem of Populus × euramericana were investigated by combined microscopic techniques.					
29938358	8	35	theme	wood	1449:1452	arg1	progresses					1461:1470	the wood growth progresses	1445:1470	the wood growth progresses	1445:1470	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	4	36	from	images	457:462	arg1	fiber					473:477	xylem fiber	467:477	xylem fiber	467:477	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	2	37	theme	combined	293:300	arg1	techniques					314:323	combined microscopic techniques	293:323	combined microscopic techniques	293:323	The topochemical variations in newly formed xylem and phloem of Populus × euramericana were investigated by combined microscopic techniques.					
29938358	1	38	theme	xylem	172:176	arg1	fiber					178:182	xylem fiber	172:182	xylem fiber	172:182	Both G units and S units deposited in the whole lignification process of xylem fiber.					
29938358	0	39	theme	dynamic	12:18	arg1	deposition					20:29	the dynamic deposition	8:29	the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana	8:96	Imaging the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana.					
29938358	0	40	from	phloem	65:70	arg1	Populus × euramericana					75:96	Populus × euramericana	75:96	Populus × euramericana	75:96	Imaging the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana.					
29938358	1	41	theme	fiber	178:182	arg1	process					161:167	the whole lignification process	137:167	the whole lignification process of xylem fiber	137:182	Both G units and S units deposited in the whole lignification process of xylem fiber.					
29938358	0	42	theme	cell	34:37	arg1	polymer					44:50	cell wall polymer	34:50	cell wall polymer	34:50	Imaging the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana.					
29938358	6	43	theme	phloem	1033:1038	arg1	location					1046:1053	phloem fiber location	1033:1053	phloem fiber location	1033:1053	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	8	44	theme	phloem	1407:1412	arg1	composition					1361:1371	cell wall composition	1351:1371	cell wall composition	1351:1371	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	8	44	theme	phloem	1407:1412	arg1	structure					1384:1392	lignin structure	1377:1392	lignin structure	1377:1392	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	5	45	theme	earlier	821:827	arg1	stage					829:833	the earlier stage	817:833	the earlier stage of lignification	817:850	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	3	46	theme	neighboring	411:421	arg1	fiber					423:427	the neighboring fiber	407:427	the neighboring fiber	407:427	During xylem formation, earlier cell wall deposition in vessel and afterwards in the neighboring fiber was observed in situ.					
29938358	0	47	from	xylem	55:59	arg1	Populus × euramericana					75:96	Populus × euramericana	75:96	Populus × euramericana	75:96	Imaging the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana.					
29938358	6	48	theme	monomers	998:1005	arg1	distribution					975:986	the distribution	971:986	the distribution of lignin monomers	971:1005	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	8	49	theme	growth	1454:1459	arg1	progresses					1461:1470	the wood growth progresses	1445:1470	the wood growth progresses	1445:1470	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	7	50	theme	S	1303:1303	arg1	lignin					1305:1310	S lignin	1303:1310	S lignin	1303:1310	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	0	51	theme	polymer	44:50	arg1	deposition					20:29	the dynamic deposition	8:29	the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana	8:96	Imaging the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana.					
29938358	5	52	theme	syringyl	731:738	arg1	units					744:748	the syringyl (S) units	727:748	the syringyl (S) units deposition	727:759	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	5	53	theme	guaiacyl	795:802	arg1	units					808:812	guaiacyl (G) units	795:812	guaiacyl (G) units	795:812	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	4	54	theme	Raman	451:455	arg1	images					457:462	Raman images	451:462	Raman images in xylem fiber	451:477	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	5	55	from	stage	829:833	arg1	pronounced					770:779	pronounced	770:779	pronounced	770:779	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	1	56	theme	G	104:104	arg1	units					106:110	G units	104:110	G units	104:110	Both G units and S units deposited in the whole lignification process of xylem fiber.					
29938358	0	57	theme	wall	39:42	arg1	polymer					44:50	cell wall polymer	34:50	cell wall polymer	34:50	Imaging the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana.					
29938358	4	58	theme	cell	495:498	arg1	process					531:537	an ordered process	520:537	an ordered process which lignification started in cell corner following carbohydrates deposition	520:615	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	4	58	theme	cell	495:498	arg1	deposition					505:514	cell wall deposition	495:514	cell wall deposition	495:514	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	6	59	theme	composition	891:901	arg1	analysis					869:876	The comparative analysis	853:876	The comparative analysis of cell wall composition in phloem fiber	853:917	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	2	60	theme	topochemical	189:200	arg1	variations					202:211	The topochemical variations	185:211	The topochemical variations in newly formed xylem and phloem of Populus × euramericana	185:270	The topochemical variations in newly formed xylem and phloem of Populus × euramericana were investigated by combined microscopic techniques.					
29938358	7	61	theme	carbohydrates	1285:1297	arg1	concentration					1268:1280	higher concentration	1261:1280	higher concentration of carbohydrates and S lignin	1261:1310	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	4	62	theme	ordered	523:529	arg1	process					531:537	an ordered process	520:537	an ordered process which lignification started in cell corner following carbohydrates deposition	520:615	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	4	62	theme	ordered	523:529	arg1	deposition					505:514	cell wall deposition	495:514	cell wall deposition	495:514	Raman images in xylem fiber emphasized that cell wall deposition was an ordered process which lignification started in cell corner following carbohydrates deposition.					
29938358	5	63	theme	cell	696:699	arg1	differentiation					706:720	the cell wall differentiation	692:720	the cell wall differentiation	692:720	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	8	64	theme	lignin	1377:1382	arg1	structure					1384:1392	lignin structure	1377:1392	lignin structure	1377:1392	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	7	65	theme	higher	1261:1266	arg1	concentration					1268:1280	higher concentration	1261:1280	higher concentration of carbohydrates and S lignin	1261:1310	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	8	66	theme	xylem	1397:1401	arg1	composition					1361:1371	cell wall composition	1351:1371	cell wall composition	1351:1371	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	8	66	theme	xylem	1397:1401	arg1	structure					1384:1392	lignin structure	1377:1392	lignin structure	1377:1392	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	5	67	theme	wall	701:704	arg1	differentiation					706:720	the cell wall differentiation	692:720	the cell wall differentiation	692:720	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	8	68	theme	woody	1502:1506	arg1	plants					1508:1513	woody plants	1502:1513	woody plants	1502:1513	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	8	69	theme	wall	1356:1359	arg1	composition					1361:1371	cell wall composition	1351:1371	cell wall composition	1351:1371	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	1	70	theme	S	116:116	arg1	units					118:122	S units	116:122	S units	116:122	Both G units and S units deposited in the whole lignification process of xylem fiber.					
29938358	3	71	theme	xylem	333:337	arg1	formation					339:347	xylem formation	333:347	xylem formation	333:347	During xylem formation, earlier cell wall deposition in vessel and afterwards in the neighboring fiber was observed in situ.					
29938358	7	72	theme	lignin	1305:1310	arg1	concentration					1268:1280	higher concentration	1261:1280	higher concentration of carbohydrates and S lignin	1261:1310	Furthermore, an interesting phenomenon was found that the outermost phloem fiber near the periderm displayed a multilayered structure with alternating broad and narrow layer, and the broad lamellae showed higher concentration of carbohydrates and S lignin.					
29938358	8	73	theme	cytological	1317:1327	arg1	information					1329:1339	The cytological information	1313:1339	The cytological information including cell wall composition and lignin structure of xylem and phloem	1313:1412	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	8	73	theme	cytological	1317:1327	arg1	helpful					1423:1429	helpful	1423:1429	helpful	1423:1429	The cytological information including cell wall composition and lignin structure of xylem and phloem might be helpful to understand the wood growth progresses and facilitate utilization of woody plants.					
29938358	5	74	theme	lignification	838:850	arg1	stage					829:833	the earlier stage	817:833	the earlier stage of lignification	817:850	Higher deposition speed of carbohydrates was revealed at the beginning of the cell wall differentiation, and the syringyl (S) units deposition was more pronounced compared with guaiacyl (G) units at the earlier stage of lignification.					
29938358	6	75	theme	wall	886:889	arg1	composition					891:901	cell wall composition	881:901	cell wall composition in phloem fiber	881:917	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
29938358	0	76	from	deposition	20:29	arg1	phloem					65:70	phloem	65:70	phloem	65:70	Imaging the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana.					
29938358	0	76	from	deposition	20:29	arg1	xylem					55:59	xylem	55:59	xylem	55:59	Imaging the dynamic deposition of cell wall polymer in xylem and phloem in Populus × euramericana.					
29938358	2	77	theme	formed	222:227	arg1	xylem					229:233	xylem	229:233	xylem	229:233	The topochemical variations in newly formed xylem and phloem of Populus × euramericana were investigated by combined microscopic techniques.					
29938358	6	78	theme	lignin	991:996	arg1	monomers					998:1005	lignin monomers	991:1005	lignin monomers	991:1005	The comparative analysis of cell wall composition in phloem fiber indicated that phloem formed earlier than xylem and the distribution of lignin monomers varied significantly with phloem fiber location.					
31867696	1	0	theme	microbiota	327:336	arg1	function					311:318	function	311:318	function	311:318	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	1	0	theme	microbiota	327:336	arg1	composition					295:305	composition	295:305	composition	295:305	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	1	1	from	function	311:318	arg1	rats					365:368	ulcerative colitis (UC) rats	341:368	ulcerative colitis (UC) rats	341:368	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	7	2	theme	serum	1279:1283	arg1	acids					1291:1295	the serum amino acids	1275:1295	the serum amino acids	1275:1295	Furthermore, the serum amino acids were regulated and showed high correlation with the gut microbiota after berberine treatment.					
31867696	1	3	theme	colitis	352:358	arg1	rats					365:368	ulcerative colitis (UC) rats	341:368	ulcerative colitis (UC) rats	341:368	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	0	4	theme	bacteria	86:93	arg1	functions					99:107	functions	99:107	functions in ulcerative colitis	99:129	Berberine ameliorates colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats.					
31867696	0	4	theme	bacteria	86:93	arg1	modulation					58:67	the modulation	54:67	the modulation of dysfunctional bacteria	54:93	Berberine ameliorates colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats.					
31867696	6	5	dep	metabolism	1106:1115	arg1	the					1102:1104	the	1102:1104	the	1102:1104	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	8	6	theme	UC	1673:1674	arg1	treatment					1676:1684	UC treatment	1673:1684	UC treatment	1673:1684	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	1	7	theme	ulcerative	341:350	arg1	UC					361:362	UC	361:362	UC	361:362	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	1	7	theme	ulcerative	341:350	arg1	colitis					352:358	ulcerative colitis	341:358	ulcerative colitis (UC) rats	341:368	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	1	8	from	composition	295:305	arg1	rats					365:368	ulcerative colitis (UC) rats	341:368	ulcerative colitis (UC) rats	341:368	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	6	9	theme	repair	1184:1189	arg1	glycolysis/gluconeogenesis					1234:1259	glycolysis/gluconeogenesis	1234:1259	glycolysis/gluconeogenesis	1234:1259	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	9	theme	repair	1184:1189	arg1	capability					1150:1159	capability	1150:1159	capability of DNA replication and repair	1150:1189	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	9	theme	repair	1184:1189	arg1	metabolism					1106:1115	metabolism	1106:1115	metabolism	1106:1115	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	9	theme	repair	1184:1189	arg1	digestion					1205:1213	carbohydrate digestion	1192:1213	carbohydrate digestion	1192:1213	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	9	theme	repair	1184:1189	arg1	biosynthesis					1121:1132	biosynthesis	1121:1132	biosynthesis	1121:1132	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	9	theme	repair	1184:1189	arg1	absorption					1219:1228	absorption	1219:1228	absorption	1219:1228	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	3	10	theme	tissues	519:525	arg1	changes					480:486	Colonic morphological changes	458:486	Colonic morphological changes	458:486	Colonic morphological changes and claudin-1 protein of colon tissues were primarily examined to validate the protective effects brought by berberine treatment.					
31867696	3	10	theme	tissues	519:525	arg1	protein					502:508	claudin-1 protein	492:508	claudin-1 protein of colon tissues	492:525	Colonic morphological changes and claudin-1 protein of colon tissues were primarily examined to validate the protective effects brought by berberine treatment.					
31867696	6	11	theme	above	1062:1066	arg1	bacteria					1068:1075	above bacteria	1062:1075	above bacteria	1062:1075	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	5	12	theme	carbohydrate	935:946	arg1	bacteria					959:966	carbohydrate hydrolysis bacteria	935:966	carbohydrate hydrolysis bacteria	935:966	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	0	13	from	modulation	58:67	arg1	colitis					123:129	ulcerative colitis	112:129	ulcerative colitis	112:129	Berberine ameliorates colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats.					
31867696	8	14	dep	bacteria	1513:1520	arg1	some					1499:1502	some	1499:1502	some	1499:1502	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	7	15	theme	amino	1285:1289	arg1	acids					1291:1295	the serum amino acids	1275:1295	the serum amino acids	1275:1295	Furthermore, the serum amino acids were regulated and showed high correlation with the gut microbiota after berberine treatment.					
31867696	6	16	theme	carbohydrate	1192:1203	arg1	digestion					1205:1213	carbohydrate digestion	1192:1213	carbohydrate digestion	1192:1213	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	17	theme	amino	1137:1141	arg1	acids					1143:1147	amino acids	1137:1147	amino acids	1137:1147	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	4	18	theme	quantitative	702:713	arg1	analysis					715:722	quantitative analysis	702:722	quantitative analysis of serum amino acids	702:743	Then the composition and function of gut microbiota were analyzed, accompanied with quantitative analysis of serum amino acids.					
31867696	5	19	theme	hydrolysis	948:957	arg1	bacteria					959:966	carbohydrate hydrolysis bacteria	935:966	carbohydrate hydrolysis bacteria	935:966	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	5	20	theme	DSS-induced	829:839	arg1	rats					844:847	DSS-induced UC rats	829:847	DSS-induced UC rats	829:847	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	4	21	dep	analyzed	675:682	arg1	accompanied					685:695	accompanied	685:695	accompanied with quantitative analysis of serum amino acids	685:743	Then the composition and function of gut microbiota were analyzed, accompanied with quantitative analysis of serum amino acids.					
31867696	4	22	theme	microbiota	659:668	arg1	composition					627:637	composition	627:637	composition	627:637	Then the composition and function of gut microbiota were analyzed, accompanied with quantitative analysis of serum amino acids.					
31867696	4	22	theme	microbiota	659:668	arg1	function					643:650	function	643:650	function	643:650	Then the composition and function of gut microbiota were analyzed, accompanied with quantitative analysis of serum amino acids.					
31867696	6	23	theme	bacteria	1068:1075	arg1	functions					1049:1057	the relevant functions	1036:1057	the relevant functions of above bacteria	1036:1075	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	0	24	theme	ulcerative	112:121	arg1	colitis					123:129	ulcerative colitis	112:129	ulcerative colitis	112:129	Berberine ameliorates colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats.					
31867696	1	25	dep	composition	295:305	arg1	the					291:293	the	291:293	the	291:293	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	8	26	from	biomarker	1636:1644	arg1	treatment					1676:1684	UC treatment	1673:1684	UC treatment	1673:1684	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	7	27	theme	gut	1349:1351	arg1	microbiota					1353:1362	the gut microbiota	1345:1362	the gut microbiota	1345:1362	Furthermore, the serum amino acids were regulated and showed high correlation with the gut microbiota after berberine treatment.					
31867696	3	28	theme	colon	513:517	arg1	tissues					519:525	colon tissues	513:525	colon tissues	513:525	Colonic morphological changes and claudin-1 protein of colon tissues were primarily examined to validate the protective effects brought by berberine treatment.					
31867696	1	29	theme	potential	178:186	arg1	pathogenesis					188:199	the potential pathogenesis	174:199	the potential pathogenesis of inflammatory bowel diseases	174:230	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	8	30	theme	microbiota	1609:1618	arg1	target					1663:1668	a therapeutic target	1649:1668	a therapeutic target in UC treatment	1649:1684	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	8	30	theme	microbiota	1609:1618	arg1	biomarker					1636:1644	a diagnostic biomarker	1623:1644	a diagnostic biomarker	1623:1644	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	8	30	theme	microbiota	1609:1618	arg1	potential					1592:1600	the potential	1588:1600	the potential of gut microbiota	1588:1618	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	4	31	theme	amino	733:737	arg1	acids					739:743	serum amino acids	727:743	serum amino acids	727:743	Then the composition and function of gut microbiota were analyzed, accompanied with quantitative analysis of serum amino acids.					
31867696	5	32	from	damages	818:824	arg1	rats					844:847	DSS-induced UC rats	829:847	DSS-induced UC rats	829:847	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	1	33	theme	pathogenesis	188:199	arg1	pathogenesis					188:199	the potential pathogenesis	174:199	the potential pathogenesis of inflammatory bowel diseases	174:230	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	1	33	theme	pathogenesis	188:199	arg1	one					167:169	one	167:169	one	167:169	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	7	34	theme	high	1323:1326	arg1	correlation					1328:1338	high correlation	1323:1338	high correlation with the gut microbiota	1323:1362	Furthermore, the serum amino acids were regulated and showed high correlation with the gut microbiota after berberine treatment.					
31867696	8	35	theme	gut	1605:1607	arg1	microbiota					1609:1618	gut microbiota	1605:1618	gut microbiota	1605:1618	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	8	36	theme	relevant	1526:1533	arg1	functions					1535:1543	relevant functions	1526:1543	relevant functions	1526:1543	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	4	37	theme	serum	727:731	arg1	acids					739:743	serum amino acids	727:743	serum amino acids	727:743	Then the composition and function of gut microbiota were analyzed, accompanied with quantitative analysis of serum amino acids.					
31867696	8	38	from	target	1663:1668	arg1	treatment					1676:1684	UC treatment	1673:1684	UC treatment	1673:1684	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	4	39	theme	gut	655:657	arg1	microbiota					659:668	gut microbiota	655:668	gut microbiota	655:668	Then the composition and function of gut microbiota were analyzed, accompanied with quantitative analysis of serum amino acids.					
31867696	5	40	theme	colonic	810:816	arg1	damages					818:824	the colonic damages	806:824	the colonic damages in DSS-induced UC rats	806:847	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	1	41	theme	berberine	278:286	arg1	effect					268:273	the effect	264:273	the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats	264:368	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	7	42	with	correlation	1328:1338	arg1	microbiota					1353:1362	the gut microbiota	1345:1362	the gut microbiota	1345:1362	Furthermore, the serum amino acids were regulated and showed high correlation with the gut microbiota after berberine treatment.					
31867696	8	43	theme	berberine	1439:1447	arg1	effect					1429:1434	the effect	1425:1434	the effect of berberine on ameliorating the colonic damage	1425:1482	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	6	44	theme	DNA	1164:1166	arg1	replication					1168:1178	DNA replication	1164:1178	DNA replication	1164:1178	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	1	45	theme	inflammatory	204:215	arg1	diseases					223:230	inflammatory bowel diseases	204:230	inflammatory bowel diseases	204:230	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	0	46	theme	colonic	22:28	arg1	damage					30:35	colonic damage	22:35	colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats	22:134	Berberine ameliorates colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats.					
31867696	1	47	from	effect	268:273	arg1	function					311:318	function	311:318	function	311:318	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	1	47	from	effect	268:273	arg1	composition					295:305	composition	295:305	composition	295:305	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	4	48	theme	acids	739:743	arg1	analysis					715:722	quantitative analysis	702:722	quantitative analysis of serum amino acids	702:743	Then the composition and function of gut microbiota were analyzed, accompanied with quantitative analysis of serum amino acids.					
31867696	1	49	theme	bowel	217:221	arg1	diseases					223:230	inflammatory bowel diseases	204:230	inflammatory bowel diseases	204:230	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	5	50	theme	acid-producing	907:920	arg1	bacteria					922:929	lactic acid-producing bacteria	900:929	lactic acid-producing bacteria	900:929	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	5	51	theme	UC	841:842	arg1	rats					844:847	DSS-induced UC rats	829:847	DSS-induced UC rats	829:847	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	0	52	dep	modulation	58:67	arg1	rats					131:134	rats	131:134	rats	131:134	Berberine ameliorates colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats.					
31867696	1	53	theme	diseases	223:230	arg1	pathogenesis					188:199	the potential pathogenesis	174:199	the potential pathogenesis of inflammatory bowel diseases	174:230	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	7	54	theme	berberine	1370:1378	arg1	treatment					1380:1388	berberine treatment	1370:1388	berberine treatment	1370:1388	Furthermore, the serum amino acids were regulated and showed high correlation with the gut microbiota after berberine treatment.					
31867696	8	55	theme	diagnostic	1625:1634	arg1	biomarker					1636:1644	a diagnostic biomarker	1623:1644	a diagnostic biomarker	1623:1644	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	8	55	theme	diagnostic	1625:1634	arg1	potential					1592:1600	the potential	1588:1600	the potential of gut microbiota	1588:1618	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	8	56	theme	specific	1504:1511	arg1	bacteria					1513:1520	specific bacteria	1504:1520	specific bacteria	1504:1520	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	4	57	dep	composition	627:637	arg1	the					623:625	the	623:625	the	623:625	Then the composition and function of gut microbiota were analyzed, accompanied with quantitative analysis of serum amino acids.					
31867696	5	58	theme	gut	871:873	arg1	microbiota					875:884	the gut microbiota	867:884	the gut microbiota	867:884	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	3	59	theme	Colonic	458:464	arg1	changes					480:486	Colonic morphological changes	458:486	Colonic morphological changes	458:486	Colonic morphological changes and claudin-1 protein of colon tissues were primarily examined to validate the protective effects brought by berberine treatment.					
31867696	8	60	theme	berberine	1557:1565	arg1	treatment					1567:1575	berberine treatment	1557:1575	berberine treatment	1557:1575	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	3	61	theme	protective	567:576	arg1	effects					578:584	the protective effects	563:584	the protective effects brought by berberine treatment	563:615	Colonic morphological changes and claudin-1 protein of colon tissues were primarily examined to validate the protective effects brought by berberine treatment.					
31867696	3	62	theme	morphological	466:478	arg1	changes					480:486	Colonic morphological changes	458:486	Colonic morphological changes	458:486	Colonic morphological changes and claudin-1 protein of colon tissues were primarily examined to validate the protective effects brought by berberine treatment.					
31867696	3	63	theme	berberine	597:605	arg1	treatment					607:615	berberine treatment	597:615	berberine treatment	597:615	Colonic morphological changes and claudin-1 protein of colon tissues were primarily examined to validate the protective effects brought by berberine treatment.					
31867696	5	64	theme	pathogenic	1002:1011	arg1	bacteria					1013:1020	conditional pathogenic bacteria	990:1020	conditional pathogenic bacteria	990:1020	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	6	65	theme	relevant	1040:1047	arg1	functions					1049:1057	the relevant functions	1036:1057	the relevant functions of above bacteria	1036:1075	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	1	66	theme	Intestinal	137:146	arg1	imbalance					154:162	Intestinal flora imbalance	137:162	Intestinal flora imbalance	137:162	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	8	67	theme	therapeutic	1651:1661	arg1	target					1663:1668	a therapeutic target	1649:1668	a therapeutic target in UC treatment	1649:1684	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	8	67	theme	therapeutic	1651:1661	arg1	potential					1592:1600	the potential	1588:1600	the potential of gut microbiota	1588:1618	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	5	68	theme	conditional	990:1000	arg1	bacteria					1013:1020	conditional pathogenic bacteria	990:1020	conditional pathogenic bacteria	990:1020	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	5	69	theme	lactic	900:905	arg1	bacteria					922:929	lactic acid-producing bacteria	900:929	lactic acid-producing bacteria	900:929	The results showed that berberine could not only ameliorate the colonic damages in DSS-induced UC rats but also regulate the gut microbiota by increasing lactic acid-producing bacteria and carbohydrate hydrolysis bacteria as well as decreasing conditional pathogenic bacteria.					
31867696	8	70	theme	colonic	1469:1475	arg1	damage					1477:1482	the colonic damage	1465:1482	the colonic damage	1465:1482	In conclusion, the study confirms the effect of berberine on ameliorating the colonic damage and highlights some specific bacteria and relevant functions linked with berberine treatment, exploring the potential of gut microbiota as a diagnostic biomarker or a therapeutic target in UC treatment.					
31867696	1	71	theme	flora	148:152	arg1	imbalance					154:162	Intestinal flora imbalance	137:162	Intestinal flora imbalance	137:162	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	6	72	theme	acids	1143:1147	arg1	glycolysis/gluconeogenesis					1234:1259	glycolysis/gluconeogenesis	1234:1259	glycolysis/gluconeogenesis	1234:1259	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	72	theme	acids	1143:1147	arg1	capability					1150:1159	capability	1150:1159	capability of DNA replication and repair	1150:1189	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	72	theme	acids	1143:1147	arg1	metabolism					1106:1115	metabolism	1106:1115	metabolism	1106:1115	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	72	theme	acids	1143:1147	arg1	digestion					1205:1213	carbohydrate digestion	1192:1213	carbohydrate digestion	1192:1213	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	72	theme	acids	1143:1147	arg1	biosynthesis					1121:1132	biosynthesis	1121:1132	biosynthesis	1121:1132	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	72	theme	acids	1143:1147	arg1	absorption					1219:1228	absorption	1219:1228	absorption	1219:1228	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	0	73	theme	dysfunctional	72:84	arg1	bacteria					86:93	dysfunctional bacteria	72:93	dysfunctional bacteria	72:93	Berberine ameliorates colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats.					
31867696	0	74	from	functions	99:107	arg1	colitis					123:129	ulcerative colitis	112:129	ulcerative colitis	112:129	Berberine ameliorates colonic damage accompanied with the modulation of dysfunctional bacteria and functions in ulcerative colitis rats.					
31867696	2	75	theme	dextran	395:401	arg1	DSS					419:421	DSS	419:421	DSS	419:421	UC rats were induced by dextran sulfate sodium (DSS) and administrated with berberine.					
31867696	2	75	theme	dextran	395:401	arg1	sodium					411:416	dextran sulfate sodium	395:416	dextran sulfate sodium (DSS)	395:422	UC rats were induced by dextran sulfate sodium (DSS) and administrated with berberine.					
31867696	3	76	theme	claudin-1	492:500	arg1	protein					502:508	claudin-1 protein	492:508	claudin-1 protein of colon tissues	492:525	Colonic morphological changes and claudin-1 protein of colon tissues were primarily examined to validate the protective effects brought by berberine treatment.					
31867696	2	77	theme	UC	371:372	arg1	rats					374:377	UC rats	371:377	UC rats	371:377	UC rats were induced by dextran sulfate sodium (DSS) and administrated with berberine.					
31867696	6	78	theme	replication	1168:1178	arg1	glycolysis/gluconeogenesis					1234:1259	glycolysis/gluconeogenesis	1234:1259	glycolysis/gluconeogenesis	1234:1259	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	78	theme	replication	1168:1178	arg1	capability					1150:1159	capability	1150:1159	capability of DNA replication and repair	1150:1189	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	78	theme	replication	1168:1178	arg1	metabolism					1106:1115	metabolism	1106:1115	metabolism	1106:1115	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	78	theme	replication	1168:1178	arg1	digestion					1205:1213	carbohydrate digestion	1192:1213	carbohydrate digestion	1192:1213	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	78	theme	replication	1168:1178	arg1	biosynthesis					1121:1132	biosynthesis	1121:1132	biosynthesis	1121:1132	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	6	78	theme	replication	1168:1178	arg1	absorption					1219:1228	absorption	1219:1228	absorption	1219:1228	Accordingly, the relevant functions of above bacteria were improved, including the metabolism and biosynthesis of amino acids, capability of DNA replication and repair, carbohydrate digestion and absorption and glycolysis/gluconeogenesis.					
31867696	1	79	theme	gut	323:325	arg1	microbiota					327:336	gut microbiota	323:336	gut microbiota	323:336	Intestinal flora imbalance is one of the potential pathogenesis of inflammatory bowel diseases, and the study aims to discover the effect of berberine on the composition and function of gut microbiota in ulcerative colitis (UC) rats.					
31867696	2	80	theme	sulfate	403:409	arg1	DSS					419:421	DSS	419:421	DSS	419:421	UC rats were induced by dextran sulfate sodium (DSS) and administrated with berberine.					
31867696	2	80	theme	sulfate	403:409	arg1	sodium					411:416	dextran sulfate sodium	395:416	dextran sulfate sodium (DSS)	395:422	UC rats were induced by dextran sulfate sodium (DSS) and administrated with berberine.					
30304011	2	0	theme	amino	527:531	arg1	ratios					538:543	ideal amino acid ratios	521:543	ideal amino acid ratios	521:543	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	3	1	theme	post-hatch	597:606	arg1	days					592:595	7 to 27 days post-hatch	584:606	7 to 27 days post-hatch	584:606	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	11	2	theme	<	1567:1567	arg1	r					1554:1554	r	1554:1554	r = 0.441	1554:1562	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	11	2	theme	<	1567:1567	arg1	P					1565:1565	P < 0.005	1565:1573	P < 0.005	1565:1573	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	6	3	theme	energy	1035:1040	arg1	densities					1042:1050	increased energy densities	1025:1050	increased energy densities (P < 0.001)	1025:1062	Feed intake was reduced with increased energy densities (P < 0.001).					
30304011	6	3	theme	energy	1035:1040	arg1	<					1055:1055	P < 0.001	1053:1061	P < 0.001	1053:1061	Feed intake was reduced with increased energy densities (P < 0.001).					
30304011	3	4	dep	days	592:595	arg1	to					586:587	to	586:587	to	586:587	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	3	5	theme	nutrient	626:633	arg1	utilisation					635:645	nutrient utilisation	626:645	nutrient utilisation (AME, AMEn, AME:GE ratios, N retention)	626:685	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	1	6	theme	experimental	175:186	arg1	diets					188:192	Twelve experimental diets	168:192	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1)	168:327	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	2	7	theme	ideal	521:525	arg1	ratios					538:543	ideal amino acid ratios	521:543	ideal amino acid ratios	521:543	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	4	8	theme	Apparent	734:741	arg1	protein					743:749	Apparent protein	734:749	Apparent protein (N)	734:753	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	4	8	theme	Apparent	734:741	arg1	N					752:752	N	752:752	N	752:752	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	13	9	theme	crystalline	2085:2095	arg1	inclusions					2108:2117	high crystalline amino acid inclusions	2080:2117	high crystalline amino acid inclusions	2080:2117	In conclusion, protein and energy need to be considered in tandem in practical diet formulation, especially in diets containing high crystalline amino acid inclusions.					
30304011	10	10	theme	high	1503:1506	arg1	P					1526:1526	P ≤ 0.001	1526:1534	P ≤ 0.001	1526:1534	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	10	10	theme	high	1503:1506	arg1	density					1517:1523	high nutrient density	1503:1523	high nutrient density (P ≤ 0.001)	1503:1535	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	11	11	dep	yield	1673:1677	arg1	=					1693:1693	=	1693:1693	=	1693:1693	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	0	12	from	utilisation	70:80	arg1	chickens					112:119	broiler chickens	104:119	broiler chickens offered diets with different energy densities	104:165	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	12	13	contain	had	1897:1899	arg2	chickens					1798:1805	broiler chickens	1790:1805	broiler chickens offered diet containing the lowest nutrient density	1790:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	13	contain	had	1897:1899	arg1	starch					1875:1880	the highest starch	1863:1880	the highest starch to lipid ratio	1863:1895	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	13	contain	had	1897:1899	arg2	concentration					1927:1939	the highest lipid carcass concentration	1901:1939	the highest lipid carcass concentration of 12.94%	1901:1949	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	14	theme	lipid	1743:1747	arg1	concentrations					1749:1762	lipid concentrations	1743:1762	lipid concentrations	1743:1762	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	10	15	located	observed	1455:1462	arg2	weights					1424:1430	Heavier carcass weights	1408:1430	Heavier carcass weights	1408:1430	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	10	15	located	observed	1455:1462	arg1	chickens					1475:1482	broiler chickens	1467:1482	broiler chickens offered diets with high nutrient density (P ≤ 0.001)	1467:1535	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	10	15	located	observed	1455:1462	arg2	yields					1443:1448	higher yields	1436:1448	higher yields	1436:1448	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	5	16	theme	growth	945:950	arg1	performance					952:962	growth performance	945:962	growth performance	945:962	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	1	17	theme	energy	215:220	arg1	densities					222:230	energy densities	215:230	energy densities (11.25, 12.38 and 13.50 MJ/kg)	215:261	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	1	17	theme	energy	215:220	arg1	MJ/kg					256:260	11.25, 12.38 and 13.50 MJ/kg	233:260	11.25, 12.38 and 13.50 MJ/kg	233:260	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	8	18	theme	feed	1234:1237	arg1	r					1247:1247	r = 0.509	1247:1255	r = 0.509	1247:1255	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	8	18	theme	feed	1234:1237	arg1	intake					1239:1244	feed intake	1234:1244	feed intake (r = 0.509, P < 0.001)	1234:1267	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	4	19	theme	digestibility	766:778	arg1	composition					812:822	composition	812:822	composition	812:822	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	4	19	theme	digestibility	766:778	arg1	coefficients					780:791	starch digestibility coefficients	759:791	starch digestibility coefficients	759:791	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	4	19	theme	digestibility	766:778	arg1	yield					802:806	carcass yield	794:806	carcass yield	794:806	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	0	20	theme	different	140:148	arg1	densities					157:165	different energy densities	140:165	different energy densities	140:165	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	8	21	dep	=	1221:1221	arg1	r					1208:1208	r = 0.448	1208:1216	r = 0.448	1208:1216	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	2	22	theme	digestible	490:499	arg1	ratios					475:480	energy ratios	468:480	energy ratios (0.87 g digestible lysine/MJ AMEn)	468:515	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	2	22	theme	digestible	490:499	arg1	AMEn					511:514	0.87 g digestible lysine/MJ AMEn	483:514	0.87 g digestible lysine/MJ AMEn	483:514	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	12	23	theme	lipid	1913:1917	arg1	concentration					1927:1939	the highest lipid carcass concentration	1901:1939	the highest lipid carcass concentration of 12.94%	1901:1949	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	23	theme	lipid	1913:1917	arg1	chickens					1798:1805	broiler chickens	1790:1805	broiler chickens offered diet containing the lowest nutrient density	1790:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	0	24	with	diets	129:133	arg1	densities					157:165	different energy densities	140:165	different energy densities	140:165	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	1	25	theme	male	349:352	arg1	chickens					371:378	288 male Ross 308 broiler chickens	345:378	288 male Ross 308 broiler chickens	345:378	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	3	26	theme	Growth	546:551	arg1	performance					553:563	Growth performance	546:563	Growth performance	546:563	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	8	27	theme	=	1249:1249	arg1	r					1247:1247	r = 0.509	1247:1255	r = 0.509	1247:1255	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	8	27	theme	=	1249:1249	arg1	intake					1239:1244	feed intake	1234:1244	feed intake (r = 0.509, P < 0.001)	1234:1267	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	11	28	theme	lipid	1617:1621	arg1	ratios					1623:1628	lipid ratios	1617:1628	lipid ratios	1617:1628	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	3	29	theme	N	674:674	arg1	retention					676:684	N retention	674:684	N retention	674:684	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	3	29	theme	N	674:674	arg1	AME					648:650	AME	648:650	AME	648:650	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	7	30	theme	Weight	1065:1070	arg1	gain					1072:1075	Weight gain	1065:1075	Weight gain	1065:1075	Weight gain and FCR were improved with increased dietary energy densities (P < 0.0001).					
30304011	5	31	theme	P	985:985	arg1	weights					976:982	carcass weights	968:982	carcass weights (P > 0.05)	968:993	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	5	31	theme	P	985:985	arg1	>					987:987	P > 0.05	985:992	P > 0.05	985:992	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	10	32	with	diets	1492:1496	arg1	P					1526:1526	P ≤ 0.001	1526:1534	P ≤ 0.001	1526:1534	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	10	32	with	diets	1492:1496	arg1	density					1517:1523	high nutrient density	1503:1523	high nutrient density (P ≤ 0.001)	1503:1535	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	8	33	dep	r	1247:1247	arg1	P					1258:1258	P < 0.001	1258:1266	P < 0.001	1258:1266	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	2	34	theme	g	488:488	arg1	ratios					475:480	energy ratios	468:480	energy ratios (0.87 g digestible lysine/MJ AMEn)	468:515	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	2	34	theme	g	488:488	arg1	AMEn					511:514	0.87 g digestible lysine/MJ AMEn	483:514	0.87 g digestible lysine/MJ AMEn	483:514	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	3	35	dep	26	714:715	arg1	to					711:712	to	711:712	to	711:712	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	8	36	theme	<	1260:1260	arg1	P					1258:1258	P < 0.001	1258:1266	P < 0.001	1258:1266	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	0	37	theme	carcass	86:92	arg1	traits					94:99	carcass traits	86:99	carcass traits	86:99	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	11	38	dep	=	1556:1556	arg1	0.441					1558:1562	0.441	1558:1562	0.441	1558:1562	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	12	39	theme	highest	1867:1873	arg1	starch					1875:1880	the highest starch	1863:1880	the highest starch to lipid ratio	1863:1895	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	11	40	dep	weight	1546:1551	arg1	r					1554:1554	r	1554:1554	r = 0.441	1554:1562	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	11	40	dep	weight	1546:1551	arg1	P					1565:1565	P < 0.005	1565:1573	P < 0.005	1565:1573	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	0	41	theme	Dietary	0:6	arg1	starch					8:13	Dietary starch	0:13	Dietary starch to lipid ratios	0:29	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	1	42	theme	Ross	354:357	arg1	chickens					371:378	288 male Ross 308 broiler chickens	345:378	288 male Ross 308 broiler chickens	345:378	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	12	43	from	interactions	1727:1738	arg1	concentrations					1749:1762	lipid concentrations	1743:1762	lipid concentrations	1743:1762	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	44	theme	lowest	1835:1840	arg1	density					1851:1857	the lowest nutrient density	1831:1857	the lowest nutrient density	1831:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	10	45	theme	carcass	1416:1422	arg1	weights					1424:1430	Heavier carcass weights	1408:1430	Heavier carcass weights	1408:1430	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	1	46	theme	fours	267:271	arg1	levels					273:278	fours levels	267:278	fours levels of starch	267:288	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	14	47	theme	feed	2143:2146	arg1	intake					2148:2153	feed intake	2143:2153	feed intake	2143:2153	The impact of lipid on feed intake and starch on carcass lipid concentrations should also be taken into consideration.					
30304011	9	48	theme	nutrient	1310:1317	arg1	densities					1319:1327	nutrient densities	1310:1327	nutrient densities	1310:1327	Both nutrient densities and starch to lipid ratios significantly impacted on carcass weight and yield.					
30304011	7	49	theme	P	1140:1140	arg1	densities					1129:1137	increased dietary energy densities	1104:1137	increased dietary energy densities (P < 0.0001)	1104:1150	Weight gain and FCR were improved with increased dietary energy densities (P < 0.0001).					
30304011	7	49	theme	P	1140:1140	arg1	<					1142:1142	P < 0.0001	1140:1149	P < 0.0001	1140:1149	Weight gain and FCR were improved with increased dietary energy densities (P < 0.0001).					
30304011	12	50	theme	broiler	1790:1796	arg1	concentration					1927:1939	the highest lipid carcass concentration	1901:1939	the highest lipid carcass concentration of 12.94%	1901:1949	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	50	theme	broiler	1790:1796	arg1	chickens					1798:1805	broiler chickens	1790:1805	broiler chickens offered diet containing the lowest nutrient density	1790:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	14	51	theme	lipid	2134:2138	arg1	impact					2124:2129	The impact	2120:2129	The impact of lipid on feed intake and starch on carcass lipid concentrations	2120:2196	The impact of lipid on feed intake and starch on carcass lipid concentrations should also be taken into consideration.					
30304011	13	52	theme	acid	2103:2106	arg1	inclusions					2108:2117	high crystalline amino acid inclusions	2080:2117	high crystalline amino acid inclusions	2080:2117	In conclusion, protein and energy need to be considered in tandem in practical diet formulation, especially in diets containing high crystalline amino acid inclusions.					
30304011	0	53	theme	growth	41:46	arg1	performance					48:58	growth performance	41:58	growth performance	41:58	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	7	54	theme	dietary	1114:1120	arg1	densities					1129:1137	increased dietary energy densities	1104:1137	increased dietary energy densities (P < 0.0001)	1104:1150	Weight gain and FCR were improved with increased dietary energy densities (P < 0.0001).					
30304011	7	54	theme	dietary	1114:1120	arg1	<					1142:1142	P < 0.0001	1140:1149	P < 0.0001	1140:1149	Weight gain and FCR were improved with increased dietary energy densities (P < 0.0001).					
30304011	14	55	from	impact	2124:2129	arg1	starch					2159:2164	starch	2159:2164	starch	2159:2164	The impact of lipid on feed intake and starch on carcass lipid concentrations should also be taken into consideration.					
30304011	14	55	from	impact	2124:2129	arg1	intake					2148:2153	feed intake	2143:2153	feed intake	2143:2153	The impact of lipid on feed intake and starch on carcass lipid concentrations should also be taken into consideration.					
30304011	14	55	from	impact	2124:2129	arg1	concentrations					2183:2196	carcass lipid concentrations	2169:2196	carcass lipid concentrations	2169:2196	The impact of lipid on feed intake and starch on carcass lipid concentrations should also be taken into consideration.					
30304011	1	56	theme	densities	222:230	arg1	levels					205:210	three levels	199:210	three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg)	199:261	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	1	56	theme	densities	222:230	arg1	levels					273:278	fours levels	267:278	fours levels of starch	267:288	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	11	57	dep	=	1693:1693	arg1	r					1680:1680	r	1680:1680	r = 0.277	1680:1688	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	10	58	theme	broiler	1467:1473	arg1	chickens					1475:1482	broiler chickens	1467:1482	broiler chickens offered diets with high nutrient density (P ≤ 0.001)	1467:1535	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	11	59	dep	ratios	1623:1628	arg1	starch					1607:1612	starch	1607:1612	starch	1607:1612	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	3	60	dep	utilisation	635:645	arg1	retention					676:684	N retention	674:684	N retention	674:684	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	3	60	dep	utilisation	635:645	arg1	AME					659:661	AME	659:661	AME	659:661	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	3	60	dep	utilisation	635:645	arg1	AMEn					653:656	AMEn	653:656	AMEn	653:656	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	3	60	dep	utilisation	635:645	arg1	AME					648:650	AME	648:650	AME	648:650	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	9	61	theme	carcass	1382:1388	arg1	weight					1390:1395	carcass weight	1382:1395	carcass weight	1382:1395	Both nutrient densities and starch to lipid ratios significantly impacted on carcass weight and yield.					
30304011	11	62	theme	Carcass	1538:1544	arg1	weight					1546:1551	Carcass weight	1538:1551	Carcass weight (r = 0.441, P < 0.005)	1538:1574	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	13	63	contain	containing	2069:2078	arg1	diets					2063:2067	diets	2063:2067	diets containing high crystalline amino acid inclusions	2063:2117	In conclusion, protein and energy need to be considered in tandem in practical diet formulation, especially in diets containing high crystalline amino acid inclusions.					
30304011	13	63	contain	containing	2069:2078	arg2	inclusions					2108:2117	high crystalline amino acid inclusions	2080:2117	high crystalline amino acid inclusions	2080:2117	In conclusion, protein and energy need to be considered in tandem in practical diet formulation, especially in diets containing high crystalline amino acid inclusions.					
30304011	1	64	theme	broiler	363:369	arg1	chickens					371:378	288 male Ross 308 broiler chickens	345:378	288 male Ross 308 broiler chickens	345:378	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	6	65	theme	increased	1025:1033	arg1	densities					1042:1050	increased energy densities	1025:1050	increased energy densities (P < 0.001)	1025:1062	Feed intake was reduced with increased energy densities (P < 0.001).					
30304011	6	65	theme	increased	1025:1033	arg1	<					1055:1055	P < 0.001	1053:1061	P < 0.001	1053:1061	Feed intake was reduced with increased energy densities (P < 0.001).					
30304011	11	66	theme	carcass	1665:1671	arg1	yield					1673:1677	carcass yield	1665:1677	carcass yield (r = 0.277, P = 0.057)	1665:1700	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	8	67	dep	gain	1202:1205	arg1	=					1221:1221	=	1221:1221	=	1221:1221	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	3	68	theme	utilisation	635:645	arg1	parameters					612:621	parameters	612:621	parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention)	612:685	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	2	69	theme	acid	533:536	arg1	ratios					538:543	ideal amino acid ratios	521:543	ideal amino acid ratios	521:543	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	13	70	theme	high	2080:2083	arg1	inclusions					2108:2117	high crystalline amino acid inclusions	2080:2117	high crystalline amino acid inclusions	2080:2117	In conclusion, protein and energy need to be considered in tandem in practical diet formulation, especially in diets containing high crystalline amino acid inclusions.					
30304011	10	71	theme	nutrient	1508:1515	arg1	P					1526:1526	P ≤ 0.001	1526:1534	P ≤ 0.001	1526:1534	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	10	71	theme	nutrient	1508:1515	arg1	density					1517:1523	high nutrient density	1503:1523	high nutrient density (P ≤ 0.001)	1503:1535	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	5	72	from	densities	905:913	arg1	weights					976:982	carcass weights	968:982	carcass weights (P > 0.05)	968:993	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	5	72	from	densities	905:913	arg1	>					987:987	P > 0.05	985:992	P > 0.05	985:992	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	5	72	from	densities	905:913	arg1	performance					952:962	growth performance	945:962	growth performance	945:962	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	12	73	theme	%	1949:1949	arg1	concentration					1927:1939	the highest lipid carcass concentration	1901:1939	the highest lipid carcass concentration of 12.94%	1901:1949	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	73	theme	%	1949:1949	arg1	chickens					1798:1805	broiler chickens	1790:1805	broiler chickens offered diet containing the lowest nutrient density	1790:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	13	74	theme	amino	2097:2101	arg1	inclusions					2108:2117	high crystalline amino acid inclusions	2080:2117	high crystalline amino acid inclusions	2080:2117	In conclusion, protein and energy need to be considered in tandem in practical diet formulation, especially in diets containing high crystalline amino acid inclusions.					
30304011	4	75	theme	carcass	794:800	arg1	coefficients					780:791	starch digestibility coefficients	759:791	starch digestibility coefficients	759:791	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	4	75	theme	carcass	794:800	arg1	yield					802:806	carcass yield	794:806	carcass yield	794:806	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	1	76	theme	lipid	293:297	arg1	ratios					299:304	lipid ratios	293:304	lipid ratios (14:1, 12:1, 7:1, 4:1)	293:327	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	0	77	theme	energy	150:155	arg1	densities					157:165	different energy densities	140:165	different energy densities	140:165	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	10	78	theme	≤	1528:1528	arg1	P					1526:1526	P ≤ 0.001	1526:1534	P ≤ 0.001	1526:1534	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	10	78	theme	≤	1528:1528	arg1	density					1517:1523	high nutrient density	1503:1523	high nutrient density (P ≤ 0.001)	1503:1535	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	2	79	theme	lysine/MJ	501:509	arg1	ratios					475:480	energy ratios	468:480	energy ratios (0.87 g digestible lysine/MJ AMEn)	468:515	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	2	79	theme	lysine/MJ	501:509	arg1	AMEn					511:514	0.87 g digestible lysine/MJ AMEn	483:514	0.87 g digestible lysine/MJ AMEn	483:514	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	12	80	theme	carcass	1919:1925	arg1	concentration					1927:1939	the highest lipid carcass concentration	1901:1939	the highest lipid carcass concentration of 12.94%	1901:1949	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	80	theme	carcass	1919:1925	arg1	chickens					1798:1805	broiler chickens	1790:1805	broiler chickens offered diet containing the lowest nutrient density	1790:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	3	81	theme	GE	663:664	arg1	ratios					666:671	GE ratios	663:671	GE ratios	663:671	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	5	82	theme	starch	919:924	arg1	ratios					935:940	starch to lipid ratios	919:940	starch to lipid ratios on growth performance and carcass weights (P > 0.05)	919:993	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	6	83	theme	Feed	996:999	arg1	intake					1001:1006	Feed intake	996:1006	Feed intake	996:1006	Feed intake was reduced with increased energy densities (P < 0.001).					
30304011	4	84	theme	starch	759:764	arg1	composition					812:822	composition	812:822	composition	812:822	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	4	84	theme	starch	759:764	arg1	coefficients					780:791	starch digestibility coefficients	759:791	starch digestibility coefficients	759:791	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	4	84	theme	starch	759:764	arg1	yield					802:806	carcass yield	794:806	carcass yield	794:806	Apparent protein (N) and starch digestibility coefficients, carcass yield and composition were determined at 27 days post-hatch.					
30304011	0	85	from	traits	94:99	arg1	chickens					112:119	broiler chickens	104:119	broiler chickens offered diets with different energy densities	104:165	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	6	86	theme	P	1053:1053	arg1	densities					1042:1050	increased energy densities	1025:1050	increased energy densities (P < 0.001)	1025:1062	Feed intake was reduced with increased energy densities (P < 0.001).					
30304011	6	86	theme	P	1053:1053	arg1	<					1055:1055	P < 0.001	1053:1061	P < 0.001	1053:1061	Feed intake was reduced with increased energy densities (P < 0.001).					
30304011	0	87	theme	nutrient	61:68	arg1	utilisation					70:80	nutrient utilisation	61:80	nutrient utilisation	61:80	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	5	88	theme	lipid	929:933	arg1	ratios					935:940	starch to lipid ratios	919:940	starch to lipid ratios on growth performance and carcass weights (P > 0.05)	919:993	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	11	89	dep	=	1682:1682	arg1	0.277					1684:1688	0.277	1684:1688	0.277	1684:1688	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	14	90	theme	lipid	2177:2181	arg1	concentrations					2183:2196	carcass lipid concentrations	2169:2196	carcass lipid concentrations	2169:2196	The impact of lipid on feed intake and starch on carcass lipid concentrations should also be taken into consideration.					
30304011	12	91	contain	containing	1820:1829	arg1	diet					1815:1818	diet	1815:1818	diet containing the lowest nutrient density	1815:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	91	contain	containing	1820:1829	arg2	density					1851:1857	the lowest nutrient density	1831:1857	the lowest nutrient density	1831:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	92	theme	highest	1905:1911	arg1	concentration					1927:1939	the highest lipid carcass concentration	1901:1939	the highest lipid carcass concentration of 12.94%	1901:1949	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	92	theme	highest	1905:1911	arg1	chickens					1798:1805	broiler chickens	1790:1805	broiler chickens offered diet containing the lowest nutrient density	1790:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	13	93	theme	practical	2021:2029	arg1	formulation					2036:2046	practical diet formulation	2021:2046	practical diet formulation	2021:2046	In conclusion, protein and energy need to be considered in tandem in practical diet formulation, especially in diets containing high crystalline amino acid inclusions.					
30304011	14	94	theme	carcass	2169:2175	arg1	concentrations					2183:2196	carcass lipid concentrations	2169:2196	carcass lipid concentrations	2169:2196	The impact of lipid on feed intake and starch on carcass lipid concentrations should also be taken into consideration.					
30304011	0	95	from	performance	48:58	arg1	chickens					112:119	broiler chickens	104:119	broiler chickens offered diets with different energy densities	104:165	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	13	96	theme	diet	2031:2034	arg1	formulation					2036:2046	practical diet formulation	2021:2046	practical diet formulation	2021:2046	In conclusion, protein and energy need to be considered in tandem in practical diet formulation, especially in diets containing high crystalline amino acid inclusions.					
30304011	12	97	theme	nutrient	1842:1849	arg1	density					1851:1857	the lowest nutrient density	1831:1857	the lowest nutrient density	1831:1857	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	0	98	theme	broiler	104:110	arg1	chickens					112:119	broiler chickens	104:119	broiler chickens offered diets with different energy densities	104:165	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	5	99	theme	carcass	968:974	arg1	weights					976:982	carcass weights	968:982	carcass weights (P > 0.05)	968:993	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	5	99	theme	carcass	968:974	arg1	>					987:987	P > 0.05	985:992	P > 0.05	985:992	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	2	100	theme	digestible	433:442	arg1	lysine					444:449	digestible lysine	433:449	digestible lysine	433:449	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	12	101	theme	lipid	1885:1889	arg1	ratio					1891:1895	lipid ratio	1885:1895	lipid ratio	1885:1895	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	8	102	theme	lipid	1163:1167	arg1	ratios					1169:1174	lipid ratios	1163:1174	lipid ratios	1163:1174	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	5	103	from	ratios	935:940	arg1	weights					976:982	carcass weights	968:982	carcass weights (P > 0.05)	968:993	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	5	103	from	ratios	935:940	arg1	>					987:987	P > 0.05	985:992	P > 0.05	985:992	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	5	103	from	ratios	935:940	arg1	performance					952:962	growth performance	945:962	growth performance	945:962	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	8	104	theme	P	1294:1294	arg1	>					1296:1296	P > 0.75	1294:1301	P > 0.75	1294:1301	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	8	104	theme	P	1294:1294	arg1	FCR					1289:1291	FCR	1289:1291	FCR (P > 0.75)	1289:1302	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	11	105	theme	=	1682:1682	arg1	r					1680:1680	r	1680:1680	r = 0.277	1680:1688	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	0	106	theme	lipid	18:22	arg1	ratios					24:29	lipid ratios	18:29	lipid ratios	18:29	Dietary starch to lipid ratios influence growth performance, nutrient utilisation and carcass traits in broiler chickens offered diets with different energy densities.					
30304011	1	107	dep	ratios	299:304	arg1	14:1					307:310	14:1	307:310	14:1	307:310	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	1	107	dep	ratios	299:304	arg1	4:1					324:326	4:1	324:326	4:1	324:326	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	1	107	dep	ratios	299:304	arg1	7:1					319:321	7:1	319:321	7:1	319:321	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	1	107	dep	ratios	299:304	arg1	12:1					313:316	12:1	313:316	12:1	313:316	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	8	108	theme	weight	1195:1200	arg1	gain					1202:1205	weight gain	1195:1205	weight gain (r = 0.448, P = 0.001)	1195:1228	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	10	109	theme	Heavier	1408:1414	arg1	weights					1424:1430	Heavier carcass weights	1408:1430	Heavier carcass weights	1408:1430	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	7	110	theme	energy	1122:1127	arg1	densities					1129:1137	increased dietary energy densities	1104:1137	increased dietary energy densities (P < 0.0001)	1104:1150	Weight gain and FCR were improved with increased dietary energy densities (P < 0.0001).					
30304011	7	110	theme	energy	1122:1127	arg1	<					1142:1142	P < 0.0001	1140:1149	P < 0.0001	1140:1149	Weight gain and FCR were improved with increased dietary energy densities (P < 0.0001).					
30304011	1	111	theme	starch	283:288	arg1	levels					205:210	three levels	199:210	three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg)	199:261	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	1	111	theme	starch	283:288	arg1	levels					273:278	fours levels	267:278	fours levels of starch	267:288	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	1	112	with	diets	188:192	arg1	levels					205:210	three levels	199:210	three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg)	199:261	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	1	112	with	diets	188:192	arg1	levels					273:278	fours levels	267:278	fours levels of starch	267:288	Twelve experimental diets with three levels of energy densities (11.25, 12.38 and 13.50 MJ/kg) and fours levels of starch to lipid ratios (14:1, 12:1, 7:1, 4:1) were offered to 288 male Ross 308 broiler chickens.					
30304011	5	113	theme	energy	898:903	arg1	densities					905:913	energy densities	898:913	energy densities	898:913	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30304011	7	114	theme	increased	1104:1112	arg1	densities					1129:1137	increased dietary energy densities	1104:1137	increased dietary energy densities (P < 0.0001)	1104:1150	Weight gain and FCR were improved with increased dietary energy densities (P < 0.0001).					
30304011	7	114	theme	increased	1104:1112	arg1	<					1142:1142	P < 0.0001	1140:1149	P < 0.0001	1140:1149	Weight gain and FCR were improved with increased dietary energy densities (P < 0.0001).					
30304011	2	115	theme	energy	468:473	arg1	ratios					475:480	energy ratios	468:480	energy ratios (0.87 g digestible lysine/MJ AMEn)	468:515	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	2	115	theme	energy	468:473	arg1	AMEn					511:514	0.87 g digestible lysine/MJ AMEn	483:514	0.87 g digestible lysine/MJ AMEn	483:514	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	8	116	theme	=	1210:1210	arg1	r					1208:1208	r = 0.448	1208:1216	r = 0.448	1208:1216	Starch to lipid ratios linearly increased weight gain (r = 0.448, P = 0.001) and feed intake (r = 0.509, P < 0.001) without influencing FCR (P > 0.75).					
30304011	10	117	theme	higher	1436:1441	arg1	yields					1443:1448	higher yields	1436:1448	higher yields	1436:1448	Heavier carcass weights and higher yields were observed in broiler chickens offered diets with high nutrient density (P ≤ 0.001).					
30304011	3	118	dep	AME	648:650	arg1	ratios					666:671	GE ratios	663:671	GE ratios	663:671	Growth performance was monitored from 7 to 27 days post-hatch and parameters of nutrient utilisation (AME, AMEn, AME:GE ratios, N retention) were determined from 24 to 26 days post-hatch.					
30304011	2	119	dep	consistent	422:431	arg1	metabolisable					454:466	metabolisable	454:466	to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios	451:543	All the diets were formulated to contain consistent digestible lysine to metabolisable energy ratios (0.87 g digestible lysine/MJ AMEn) and ideal amino acid ratios.					
30304011	9	120	theme	lipid	1343:1347	arg1	ratios					1349:1354	lipid ratios	1343:1354	lipid ratios	1343:1354	Both nutrient densities and starch to lipid ratios significantly impacted on carcass weight and yield.					
30304011	12	121	theme	P	1776:1776	arg1	carcass					1767:1773	carcass	1767:1773	carcass (P < 0.001)	1767:1785	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	12	121	theme	P	1776:1776	arg1	<					1778:1778	P < 0.001	1776:1784	P < 0.001	1776:1784	However, there were interactions on lipid concentrations in carcass (P < 0.001) as broiler chickens offered diet containing the lowest nutrient density and the highest starch to lipid ratio had the highest lipid carcass concentration of 12.94%.					
30304011	11	122	theme	=	1556:1556	arg1	r					1554:1554	r	1554:1554	r = 0.441	1554:1562	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	11	122	theme	=	1556:1556	arg1	P					1565:1565	P < 0.005	1565:1573	P < 0.005	1565:1573	Carcass weight (r = 0.441, P < 0.005) was positively correlated with starch to lipid ratios and this tended to be the case for carcass yield (r = 0.277, P = 0.057) too.					
30304011	5	123	dep	ratios	935:940	arg1	to					926:927	to	926:927	to	926:927	There were no interactions between energy densities and starch to lipid ratios on growth performance and carcass weights (P > 0.05).					
30353341	2	0	theme	shockogenic	365:375	arg1	thrombocytopathy					377:392	shockogenic thrombocytopathy	365:392	shockogenic thrombocytopathy	365:392	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
30353341	0	1	from	Mechanisms	23:32	arg1	Rabbits					61:67	Rabbits	61:67	Rabbits with Hemorrhagic Shock	61:90	Phospholipid-Dependent Mechanisms of Platelet Dysfunction in Rabbits with Hemorrhagic Shock.					
30353341	1	2	from	peak	173:176	arg1	composition					125:135	phospholipid composition	112:135	phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock	112:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	1	2	from	peak	173:176	arg1	membranes					156:164	platelet plasma membranes	140:164	platelet plasma membranes at the peak of hemorrhagic shock	140:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	2	3	theme	structure	290:298	arg1	impairment					272:281	impairment	272:281	impairment of the structure of platelet membrane phospholipid bilayer	272:340	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
30353341	2	4	theme	phospholipid	321:332	arg1	bilayer					334:340	platelet membrane phospholipid bilayer	303:340	platelet membrane phospholipid bilayer	303:340	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
30353341	2	5	theme	bilayer	334:340	arg1	structure					290:298	the structure	286:298	the structure of platelet membrane phospholipid bilayer	286:340	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
30353341	2	6	theme	impairment	272:281	arg1	role					264:267	the key role	256:267	the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy	256:392	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
30353341	1	7	theme	hemorrhagic	181:191	arg1	shock					193:197	hemorrhagic shock	181:197	hemorrhagic shock	181:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	1	8	theme	time	97:100	arg1	course					102:107	The time course	93:107	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock	93:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	1	9	theme	shock	193:197	arg1	peak					173:176	the peak	169:176	the peak of hemorrhagic shock	169:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	0	10	theme	Phospholipid-Dependent	0:21	arg1	Mechanisms					23:32	Phospholipid-Dependent Mechanisms	0:32	Phospholipid-Dependent Mechanisms of Platelet Dysfunction in Rabbits with Hemorrhagic Shock	0:90	Phospholipid-Dependent Mechanisms of Platelet Dysfunction in Rabbits with Hemorrhagic Shock.					
30353341	1	11	from	composition	125:135	arg1	peak					173:176	the peak	169:176	the peak of hemorrhagic shock	169:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	2	12	theme	membrane	312:319	arg1	bilayer					334:340	platelet membrane phospholipid bilayer	303:340	platelet membrane phospholipid bilayer	303:340	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
30353341	0	13	theme	Platelet	37:44	arg1	Dysfunction					46:56	Platelet Dysfunction	37:56	Platelet Dysfunction	37:56	Phospholipid-Dependent Mechanisms of Platelet Dysfunction in Rabbits with Hemorrhagic Shock.					
30353341	2	14	theme	platelet	303:310	arg1	bilayer					334:340	platelet membrane phospholipid bilayer	303:340	platelet membrane phospholipid bilayer	303:340	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
30353341	1	15	theme	phospholipid	112:123	arg1	composition					125:135	phospholipid composition	112:135	phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock	112:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	2	16	from	role	264:267	arg1	pathogenesis					349:360	the pathogenesis	345:360	the pathogenesis of shockogenic thrombocytopathy	345:392	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
30353341	1	17	theme	rabbit	215:220	arg1	experiments					222:232	rabbit experiments	215:232	rabbit experiments	215:232	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	0	18	theme	Dysfunction	46:56	arg1	Mechanisms					23:32	Phospholipid-Dependent Mechanisms	0:32	Phospholipid-Dependent Mechanisms of Platelet Dysfunction in Rabbits with Hemorrhagic Shock	0:90	Phospholipid-Dependent Mechanisms of Platelet Dysfunction in Rabbits with Hemorrhagic Shock.					
30353341	1	19	theme	composition	125:135	arg1	course					102:107	The time course	93:107	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock	93:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	1	20	theme	platelet	140:147	arg1	membranes					156:164	platelet plasma membranes	140:164	platelet plasma membranes at the peak of hemorrhagic shock	140:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	1	21	theme	plasma	149:154	arg1	membranes					156:164	platelet plasma membranes	140:164	platelet plasma membranes at the peak of hemorrhagic shock	140:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	2	22	theme	key	260:262	arg1	role					264:267	the key role	256:267	the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy	256:392	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
30353341	0	23	with	Rabbits	61:67	arg1	Shock					86:90	Hemorrhagic Shock	74:90	Hemorrhagic Shock	74:90	Phospholipid-Dependent Mechanisms of Platelet Dysfunction in Rabbits with Hemorrhagic Shock.					
30353341	1	24	theme	membranes	156:164	arg1	composition					125:135	phospholipid composition	112:135	phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock	112:197	The time course of phospholipid composition of platelet plasma membranes at the peak of hemorrhagic shock were studied in rabbit experiments.					
30353341	0	25	theme	Hemorrhagic	74:84	arg1	Shock					86:90	Hemorrhagic Shock	74:90	Hemorrhagic Shock	74:90	Phospholipid-Dependent Mechanisms of Platelet Dysfunction in Rabbits with Hemorrhagic Shock.					
30353341	2	26	theme	thrombocytopathy	377:392	arg1	pathogenesis					349:360	the pathogenesis	345:360	the pathogenesis of shockogenic thrombocytopathy	345:392	The results indicate the key role of impairment of the structure of platelet membrane phospholipid bilayer in the pathogenesis of shockogenic thrombocytopathy.					
29588196	0	0	theme	alginate-loofah	91:105	arg1	matrices					107:114	alginate-loofah matrices	91:114	alginate-loofah matrices	91:114	Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices.					
29588196	5	1	theme	ALM-immobilized	805:819	arg1	cells					821:825	ALM-immobilized cells	805:825	ALM-immobilized cells	805:825	The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively.					
29588196	6	2	theme	cells	929:933	arg1	successful					939:948	successful	939:948	successful	939:948	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	6	2	theme	cells	929:933	arg1	use					902:904	The use	898:904	The use of the ALM-immobilized cells	898:933	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	2	3	theme	20×20×5mm3	359:368	arg1	dimensions					345:354	dimensions	345:354	dimensions of 20×20×5mm3	345:368	As found in this study, an ALM with dimensions of 20×20×5mm3 is effective for cell immobilization due to its compact structure and long-term stability.					
29588196	4	4	theme	composite	599:607	arg1	CCD					617:619	CCD	617:619	CCD	617:619	By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined.					
29588196	4	4	theme	composite	599:607	arg1	design					609:614	a central composite design	589:614	a central composite design (CCD)	589:620	By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined.					
29588196	6	5	from	application	1072:1082	arg1	production					1106:1115	industrial ethanol production	1087:1115	industrial ethanol production	1087:1115	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	6	6	theme	production	1022:1031	arg1	efficiency					1033:1042	ethanol production efficiency	1014:1042	ethanol production efficiency	1014:1042	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	5	7	theme	volumetric	758:767	arg1	productivity					777:788	volumetric ethanol productivity	758:788	volumetric ethanol productivity	758:788	The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively.					
29588196	6	8	theme	ethanol	1014:1020	arg1	efficiency					1033:1042	ethanol production efficiency	1014:1042	ethanol production efficiency	1014:1042	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	5	9	theme	ethanol	769:775	arg1	productivity					777:788	volumetric ethanol productivity	758:788	volumetric ethanol productivity	758:788	The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively.					
29588196	6	10	theme	ethanol	1098:1104	arg1	production					1106:1115	industrial ethanol production	1087:1115	industrial ethanol production	1087:1115	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	3	11	theme	suspended	557:565	arg1	system					572:577	the freely suspended cell system	546:577	the freely suspended cell system	546:577	The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system.					
29588196	1	12	theme	Saccharomyces	192:204	arg1	strain					217:222	the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53	173:232	the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM)	173:279	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	3	13	theme	cell	567:570	arg1	system					572:577	the freely suspended cell system	546:577	the freely suspended cell system	546:577	The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system.					
29588196	4	14	from	SSJ	674:676	arg1	production					658:667	ethanol production	650:667	ethanol production from SSJ by ALM-immobilized cells	650:701	By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined.					
29588196	1	15	theme	thermotolerant	177:190	arg1	strain					217:222	the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53	173:232	the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM)	173:279	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	0	16	from	sorghum	30:36	arg1	production					8:17	Ethanol production	0:17	Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53	0:74	Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices.					
29588196	1	17	from	juice	155:159	arg1	production					125:134	Ethanol production	117:134	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM)	117:279	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	2	18	theme	cell	387:390	arg1	immobilization					392:405	cell immobilization	387:405	cell immobilization due to its compact structure and long-term stability	387:458	As found in this study, an ALM with dimensions of 20×20×5mm3 is effective for cell immobilization due to its compact structure and long-term stability.					
29588196	0	19	theme	Ethanol	0:6	arg1	production					8:17	Ethanol production	0:17	Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53	0:74	Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices.					
29588196	0	20	theme	sweet	24:28	arg1	sorghum					30:36	sweet sorghum	24:36	sweet sorghum	24:36	Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices.					
29588196	1	21	theme	Ethanol	117:123	arg1	production					125:134	Ethanol production	117:134	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM)	117:279	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	3	22	theme	cell	481:484	arg1	system					486:491	The ALM-immobilized cell system	461:491	The ALM-immobilized cell system	461:491	The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system.					
29588196	4	23	theme	central	591:597	arg1	CCD					617:619	CCD	617:619	CCD	617:619	By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined.					
29588196	4	23	theme	central	591:597	arg1	design					609:614	a central composite design	589:614	a central composite design (CCD)	589:620	By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined.					
29588196	5	24	theme	maximum	724:730	arg1	concentration					740:752	maximum ethanol concentration	724:752	maximum ethanol concentration	724:752	The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively.					
29588196	2	25	with	ALM	336:338	arg1	dimensions					345:354	dimensions	345:354	dimensions of 20×20×5mm3	345:368	As found in this study, an ALM with dimensions of 20×20×5mm3 is effective for cell immobilization due to its compact structure and long-term stability.					
29588196	2	26	theme	compact	418:424	arg1	structure					426:434	compact structure	418:434	compact structure	418:434	As found in this study, an ALM with dimensions of 20×20×5mm3 is effective for cell immobilization due to its compact structure and long-term stability.					
29588196	3	27	theme	production	519:528	arg1	efficiency					530:539	greater ethanol production efficiency	503:539	greater ethanol production efficiency than the freely suspended cell system	503:577	The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system.					
29588196	5	28	theme	ethanol	732:738	arg1	concentration					740:752	maximum ethanol concentration	724:752	maximum ethanol concentration	724:752	The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively.					
29588196	1	29	dep	Saccharomyces	192:204	arg1	cerevisiae					206:215	cerevisiae	206:215	cerevisiae	206:215	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	6	30	theme	industrial	1087:1096	arg1	production					1106:1115	industrial ethanol production	1087:1115	industrial ethanol production	1087:1115	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	6	31	theme	efficiency	1033:1042	arg1	loss					1006:1009	any loss	1002:1009	any loss of ethanol production efficiency	1002:1042	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	2	32	theme	long-term	440:448	arg1	stability					450:458	long-term stability	440:458	long-term stability	440:458	As found in this study, an ALM with dimensions of 20×20×5mm3 is effective for cell immobilization due to its compact structure and long-term stability.					
29588196	3	33	theme	ALM-immobilized	465:479	arg1	system					486:491	The ALM-immobilized cell system	461:491	The ALM-immobilized cell system	461:491	The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system.					
29588196	5	34	theme	optimal	837:843	arg1	conditions					845:854	the optimal conditions	833:854	the optimal conditions	833:854	The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively.					
29588196	4	35	theme	ALM-immobilized	681:695	arg1	cells					697:701	ALM-immobilized cells	681:701	ALM-immobilized cells	681:701	By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined.					
29588196	6	36	theme	ALM-immobilized	913:927	arg1	cells					929:933	the ALM-immobilized cells	909:933	the ALM-immobilized cells	909:933	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	3	37	theme	ethanol	511:517	arg1	efficiency					530:539	greater ethanol production efficiency	503:539	greater ethanol production efficiency than the freely suspended cell system	503:577	The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system.					
29588196	0	38	theme	Saccharomyces	41:53	arg1	cerevisiae					55:64	Saccharomyces cerevisiae DBKKUY-53	41:74	Saccharomyces cerevisiae DBKKUY-53	41:74	Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices.					
29588196	1	39	theme	sweet	141:145	arg1	SSJ					162:164	SSJ	162:164	SSJ	162:164	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	1	39	theme	sweet	141:145	arg1	juice					155:159	sweet sorghum juice	141:159	sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM)	141:279	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	6	40	theme	potential	1062:1070	arg1	application					1072:1082	their potential application	1056:1082	their potential application in industrial ethanol production	1056:1115	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	1	41	theme	sorghum	147:153	arg1	SSJ					162:164	SSJ	162:164	SSJ	162:164	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	1	41	theme	sorghum	147:153	arg1	juice					155:159	sweet sorghum juice	141:159	sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM)	141:279	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	3	42	theme	greater	503:509	arg1	efficiency					530:539	greater ethanol production efficiency	503:539	greater ethanol production efficiency than the freely suspended cell system	503:577	The ALM-immobilized cell system exhibited greater ethanol production efficiency than the freely suspended cell system.					
29588196	1	43	theme	alginate-loofah	252:266	arg1	matrix					268:273	an alginate-loofah matrix	249:273	an alginate-loofah matrix (ALM)	249:279	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	1	43	theme	alginate-loofah	252:266	arg1	ALM					276:278	ALM	276:278	ALM	276:278	Ethanol production from sweet sorghum juice (SSJ) using the thermotolerant Saccharomyces cerevisiae strain DBKKUY-53 immobilized in an alginate-loofah matrix (ALM) was successfully developed.					
29588196	4	44	theme	ethanol	650:656	arg1	production					658:667	ethanol production	650:667	ethanol production from SSJ by ALM-immobilized cells	650:701	By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined.					
29588196	4	45	theme	optimum	627:633	arg1	conditions					635:644	the optimum conditions	623:644	the optimum conditions for ethanol production from SSJ by ALM-immobilized cells	623:701	By using a central composite design (CCD), the optimum conditions for ethanol production from SSJ by ALM-immobilized cells were determined.					
29588196	5	46	dep	concentration	740:752	arg1	The					720:722	The	720:722	The	720:722	The maximum ethanol concentration and volumetric ethanol productivity obtained using ALM-immobilized cells under the optimal conditions were 97.54g/L and 1.36g/Lh, respectively.					
29588196	6	47	theme	consecutive	967:977	arg1	360h					988:991	360h	988:991	360h	988:991	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
29588196	6	47	theme	consecutive	967:977	arg1	batches					979:985	at least six consecutive batches	954:985	at least six consecutive batches (360h)	954:992	The use of the ALM-immobilized cells was successful for at least six consecutive batches (360h) without any loss of ethanol production efficiency, suggesting their potential application in industrial ethanol production.					
31638775	5	0	dep	factors	837:843	arg1	temperature					850:860	temperature	850:860	temperature	850:860	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	0	dep	factors	837:843	arg1	use					867:869	use	867:869	use of catalyst	867:881	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	0	dep	factors	837:843	arg1	pH					846:847	pH	846:847	pH	846:847	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	0	dep	factors	837:843	arg1	factors					837:843	environmental factors	823:843	environmental factors (pH, temperature, and use of catalyst)	823:882	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	7	1	from	encapsulation	1328:1340	arg1	hydrogels					1354:1362	hydrogels	1354:1362	hydrogels (fast calcium cross-linking)	1354:1391	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	1	from	encapsulation	1328:1340	arg1	cross-linking					1378:1390	fast calcium cross-linking	1365:1390	fast calcium cross-linking	1365:1390	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	5	2	theme	mechanical	667:676	arg1	properties					678:687	highly tunable stress relaxation and mechanical properties	630:687	properties	678:687	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	7	3	theme	extended	1476:1483	arg1	cross-linking					1507:1519	durable oxime cross-linking	1493:1519	durable oxime cross-linking	1493:1519	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	3	theme	extended	1476:1483	arg1	period					1485:1490	an extended period	1473:1490	an extended period (durable oxime cross-linking)	1473:1520	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	4	theme	viscoelastic	1425:1436	arg1	environments					1438:1449	controlled viscoelastic environments	1414:1449	controlled viscoelastic environments	1414:1449	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	8	5	theme	hydrogel	1642:1649	arg1	platform					1651:1658	this novel hydrogel platform	1631:1658	this novel hydrogel platform	1631:1658	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	2	6	theme	sodium	282:287	arg1	NaAlg					299:303	NaAlg	299:303	NaAlg	299:303	Here, we report a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation.					
31638775	2	6	theme	sodium	282:287	arg1	alginate					289:296	sodium alginate	282:296	sodium alginate (NaAlg) with tunable stress relaxation	282:335	Here, we report a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation.					
31638775	8	7	theme	stress	1715:1720	arg1	relaxation					1722:1731	stress relaxation	1715:1731	stress relaxation	1715:1731	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	5	8	theme	tunable	637:643	arg1	relaxation					652:661	highly tunable stress relaxation and mechanical properties	630:687	relaxation	652:661	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	1	9	theme	natural	195:201	arg1	ECM					225:227	ECM	225:227	ECM	225:227	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	1	9	theme	natural	195:201	arg1	matrix					217:222	natural extracellular matrix	195:222	natural extracellular matrix (ECM)	195:228	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	7	10	theme	cultured	1454:1461	arg1	cells					1463:1467	cultured cells	1454:1467	cultured cells	1454:1467	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	8	11	theme	key	1741:1743	arg1	role					1745:1748	a key role	1739:1748	a key role	1739:1748	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	5	12	theme	catalyst	874:881	arg1	temperature					850:860	temperature	850:860	temperature	850:860	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	12	theme	catalyst	874:881	arg1	use					867:869	use	867:869	use of catalyst	867:881	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	12	theme	catalyst	874:881	arg1	pH					846:847	pH	846:847	pH	846:847	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	12	theme	catalyst	874:881	arg1	factors					837:843	environmental factors	823:843	environmental factors (pH, temperature, and use of catalyst)	823:882	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	6	13	theme	divalent	960:967	arg1	cations					969:975	divalent cations	960:975	divalent cations	960:975	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	7	14	from	creation	1193:1200	arg1	microbead					1218:1226	microbead	1218:1226	microbead	1218:1226	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	14	from	creation	1193:1200	arg1	geometries					1243:1252	microthread geometries	1231:1252	microthread geometries	1231:1252	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	15	theme	calcium	1370:1376	arg1	hydrogels					1354:1362	hydrogels	1354:1362	hydrogels (fast calcium cross-linking)	1354:1391	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	15	theme	calcium	1370:1376	arg1	cross-linking					1378:1390	fast calcium cross-linking	1365:1390	fast calcium cross-linking	1365:1390	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	16	theme	cross-linking	1140:1152	arg1	capability					1154:1163	this dual cross-linking capability	1130:1163	this dual cross-linking capability	1130:1163	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	17	from	hydrogels	1205:1213	arg1	microbead					1218:1226	microbead	1218:1226	microbead	1218:1226	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	17	from	hydrogels	1205:1213	arg1	geometries					1243:1252	microthread geometries	1231:1252	microthread geometries	1231:1252	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	1	18	theme	biomaterials	124:135	arg1	relaxation					77:86	Stress relaxation	70:86	Stress relaxation	70:86	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	1	18	theme	biomaterials	124:135	arg1	parameter					111:119	an important design parameter	91:119	an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM)	91:228	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	7	19	from	microbead	1218:1226	arg1	creation					1193:1200	the creation	1189:1200	the creation of hydrogels in microbead or microthread geometries	1189:1252	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	2	20	theme	hydrogel	255:262	arg1	platform					264:271	a novel hydrogel platform	247:271	a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation	247:335	Here, we report a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation.					
31638775	5	21	dep	composition	735:745	arg1	concentration					748:760	concentration	748:760	concentration	748:760	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	21	dep	composition	735:745	arg1	degree					786:791	degree	786:791	degree of oxidation of NaAlg-Ald	786:817	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	21	dep	composition	735:745	arg1	ratios					778:783	polymer mixing ratios	763:783	polymer mixing ratios	763:783	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	7	22	theme	microthread	1231:1241	arg1	geometries					1243:1252	microthread geometries	1231:1252	microthread geometries	1231:1252	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	6	23	theme	developed	982:990	arg1	formations					1001:1010	the developed hydrogel formations	978:1010	the developed hydrogel formations	978:1010	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	7	24	dep	utilized	1176:1183	arg1	i					1170:1170	i	1170:1170	i	1170:1170	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	8	25	from	good	1671:1674	arg1	applications					1696:1707	various applications	1688:1707	various applications where stress relaxation plays a key role in cell-matrix interactions	1688:1776	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	7	26	theme	hydrogels	1205:1213	arg1	creation					1193:1200	the creation	1189:1200	the creation of hydrogels in microbead or microthread geometries	1189:1252	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	1	27	theme	Stress	70:75	arg1	relaxation					77:86	Stress relaxation	70:86	Stress relaxation	70:86	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	1	27	theme	Stress	70:75	arg1	parameter					111:119	an important design parameter	91:119	an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM)	91:228	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	3	28	theme	alginate	431:438	arg1	backbone					448:455	the alginate polymer backbone	427:455	the alginate polymer backbone	427:455	We first developed a new synthesis route to introduce alkoxyamine functional groups into the alginate polymer backbone.					
31638775	5	29	theme	mixing	771:776	arg1	concentration					748:760	concentration	748:760	concentration	748:760	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	29	theme	mixing	771:776	arg1	ratios					778:783	polymer mixing ratios	763:783	polymer mixing ratios	763:783	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	30	theme	oxidation	796:804	arg1	concentration					748:760	concentration	748:760	concentration	748:760	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	30	theme	oxidation	796:804	arg1	degree					786:791	degree	786:791	degree of oxidation of NaAlg-Ald	786:817	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	6	31	theme	different	1080:1088	arg1	kinetics					1099:1106	different gelation kinetics	1080:1106	different gelation kinetics	1080:1106	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	8	32	from	applications	1696:1707	arg1	good					1671:1674	good	1671:1674	good	1671:1674	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	5	33	theme	environmental	823:835	arg1	temperature					850:860	temperature	850:860	temperature	850:860	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	33	theme	environmental	823:835	arg1	use					867:869	use	867:869	use of catalyst	867:881	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	33	theme	environmental	823:835	arg1	pH					846:847	pH	846:847	pH	846:847	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	33	theme	environmental	823:835	arg1	factors					837:843	environmental factors	823:843	environmental factors (pH, temperature, and use of catalyst)	823:882	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	1	34	theme	important	94:102	arg1	relaxation					77:86	Stress relaxation	70:86	Stress relaxation	70:86	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	1	34	theme	important	94:102	arg1	parameter					111:119	an important design parameter	91:119	an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM)	91:228	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	6	35	theme	mechanisms	1064:1073	arg1	capability					1031:1040	the unique capability	1020:1040	the unique capability of dual cross-linking mechanisms with different gelation kinetics	1020:1106	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	3	36	theme	new	359:361	arg1	route					373:377	a new synthesis route	357:377	a new synthesis route to introduce alkoxyamine functional groups into the alginate polymer backbone	357:455	We first developed a new synthesis route to introduce alkoxyamine functional groups into the alginate polymer backbone.					
31638775	0	37	theme	Alginate	19:26	arg1	Hydrogels					28:36	Alginate Hydrogels	19:36	Alginate Hydrogels	19:36	Oxime Cross-Linked Alginate Hydrogels with Tunable Stress Relaxation.					
31638775	6	38	theme	dual	1045:1048	arg1	mechanisms					1064:1073	dual cross-linking mechanisms	1045:1073	dual cross-linking mechanisms with different gelation kinetics	1045:1106	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	3	39	theme	functional	404:413	arg1	groups					415:420	alkoxyamine functional groups	392:420	alkoxyamine functional groups	392:420	We first developed a new synthesis route to introduce alkoxyamine functional groups into the alginate polymer backbone.					
31638775	4	40	theme	oxime	557:561	arg1	hydrogels					585:593	oxime cross-linked alginate hydrogels	557:593	oxime cross-linked alginate hydrogels	557:593	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	7	41	used	utilized	1176:1183	arg2	capability					1154:1163	this dual cross-linking capability	1130:1163	this dual cross-linking capability	1130:1163	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	4	42	theme	oxidized	526:533	arg1	NaAlg-Ald					545:553	NaAlg-Ald	545:553	NaAlg-Ald	545:553	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	4	42	theme	oxidized	526:533	arg1	alginate					535:542	aldehyde-containing oxidized alginate	506:542	aldehyde-containing oxidized alginate (NaAlg-Ald)	506:554	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	0	43	theme	Stress	51:56	arg1	Relaxation					58:67	Tunable Stress Relaxation	43:67	Tunable Stress Relaxation	43:67	Oxime Cross-Linked Alginate Hydrogels with Tunable Stress Relaxation.					
31638775	8	44	theme	line	1582:1585	arg1	culture					1562:1568	the culture	1558:1568	the culture of a B-cell line encapsulated within the developed hydrogel	1558:1628	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	2	45	theme	stress	319:324	arg1	relaxation					326:335	tunable stress relaxation	311:335	tunable stress relaxation	311:335	Here, we report a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation.					
31638775	7	46	theme	durable	1493:1499	arg1	cross-linking					1507:1519	durable oxime cross-linking	1493:1519	durable oxime cross-linking	1493:1519	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	46	theme	durable	1493:1499	arg1	period					1485:1490	an extended period	1473:1490	an extended period (durable oxime cross-linking)	1473:1520	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	4	47	link	cross-linked	563:574	arg1	hydrogels					585:593	oxime cross-linked alginate hydrogels	557:593	oxime cross-linked alginate hydrogels	557:593	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	8	48	theme	developed	1611:1619	arg1	hydrogel					1621:1628	the developed hydrogel	1607:1628	the developed hydrogel	1607:1628	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	7	49	theme	dual	1135:1138	arg1	capability					1154:1163	this dual cross-linking capability	1130:1163	this dual cross-linking capability	1130:1163	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	6	50	theme	natural	903:909	arg1	capability					911:920	the natural capability	899:920	the natural capability of the alginate to be cross-linked by divalent cations	899:975	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	7	51	from	geometries	1243:1252	arg1	creation					1193:1200	the creation	1189:1200	the creation of hydrogels in microbead or microthread geometries	1189:1252	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	4	52	theme	alginate	576:583	arg1	hydrogels					585:593	oxime cross-linked alginate hydrogels	557:593	oxime cross-linked alginate hydrogels	557:593	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	8	53	theme	various	1688:1694	arg1	applications					1696:1707	various applications	1688:1707	various applications where stress relaxation plays a key role in cell-matrix interactions	1688:1776	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	7	54	theme	environments	1438:1449	arg1	provision					1401:1409	the provision	1397:1409	the provision of controlled viscoelastic environments to cultured cells	1397:1467	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	54	theme	environments	1438:1449	arg1	encapsulation					1328:1340	the fast encapsulation	1319:1340	the fast encapsulation of cells in hydrogels (fast calcium cross-linking)	1319:1391	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	8	55	theme	novel	1636:1640	arg1	platform					1651:1658	this novel hydrogel platform	1631:1658	this novel hydrogel platform	1631:1658	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	7	56	theme	controlled	1414:1423	arg1	environments					1438:1449	controlled viscoelastic environments	1414:1449	controlled viscoelastic environments	1414:1449	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	5	57	theme	stress	645:650	arg1	relaxation					652:661	highly tunable stress relaxation and mechanical properties	630:687	relaxation	652:661	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	1	58	theme	extracellular	203:215	arg1	ECM					225:227	ECM	225:227	ECM	225:227	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	1	58	theme	extracellular	203:215	arg1	matrix					217:222	natural extracellular matrix	195:222	natural extracellular matrix (ECM)	195:228	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	8	59	theme	cell-matrix	1753:1763	arg1	interactions					1765:1776	cell-matrix interactions	1753:1776	cell-matrix interactions	1753:1776	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	6	60	theme	alginate	929:936	arg1	capability					911:920	the natural capability	899:920	the natural capability of the alginate to be cross-linked by divalent cations	899:975	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	7	61	theme	fast	1365:1368	arg1	hydrogels					1354:1362	hydrogels	1354:1362	hydrogels (fast calcium cross-linking)	1354:1391	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	61	theme	fast	1365:1368	arg1	cross-linking					1378:1390	fast calcium cross-linking	1365:1390	fast calcium cross-linking	1365:1390	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	6	62	theme	unique	1024:1029	arg1	capability					1031:1040	the unique capability	1020:1040	the unique capability of dual cross-linking mechanisms with different gelation kinetics	1020:1106	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	8	63	dep	good	1671:1674	arg1	prospect					1676:1683	prospect	1676:1683	prospect	1676:1683	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	2	64	theme	novel	249:253	arg1	platform					264:271	a novel hydrogel platform	247:271	a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation	247:335	Here, we report a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation.					
31638775	7	65	from	provision	1401:1409	arg1	hydrogels					1354:1362	hydrogels	1354:1362	hydrogels (fast calcium cross-linking)	1354:1391	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	65	from	provision	1401:1409	arg1	cross-linking					1378:1390	fast calcium cross-linking	1365:1390	fast calcium cross-linking	1365:1390	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	2	66	with	alginate	289:296	arg1	relaxation					326:335	tunable stress relaxation	311:335	tunable stress relaxation	311:335	Here, we report a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation.					
31638775	7	67	theme	cells	1345:1349	arg1	provision					1401:1409	the provision	1397:1409	the provision of controlled viscoelastic environments to cultured cells	1397:1467	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	67	theme	cells	1345:1349	arg1	encapsulation					1328:1340	the fast encapsulation	1319:1340	the fast encapsulation of cells in hydrogels (fast calcium cross-linking)	1319:1391	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	1	68	theme	artificial	157:166	arg1	microenvironment					168:183	an artificial microenvironment	154:183	an artificial microenvironment mimicking natural extracellular matrix (ECM)	154:228	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	6	69	theme	hydrogel	992:999	arg1	formations					1001:1010	the developed hydrogel formations	978:1010	the developed hydrogel formations	978:1010	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	4	70	theme	aldehyde-containing	506:524	arg1	NaAlg-Ald					545:553	NaAlg-Ald	545:553	NaAlg-Ald	545:553	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	4	70	theme	aldehyde-containing	506:524	arg1	alginate					535:542	aldehyde-containing oxidized alginate	506:542	aldehyde-containing oxidized alginate (NaAlg-Ald)	506:554	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	4	71	theme	resulting	472:480	arg1	polymer					482:488	the resulting polymer	468:488	the resulting polymer (NaAlg-AA)	468:499	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	4	71	theme	resulting	472:480	arg1	NaAlg-AA					491:498	NaAlg-AA	491:498	NaAlg-AA	491:498	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	6	72	contain	possess	1012:1018	arg2	capability					1031:1040	the unique capability	1020:1040	the unique capability of dual cross-linking mechanisms with different gelation kinetics	1020:1106	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	6	72	contain	possess	1012:1018	arg1	formations					1001:1010	the developed hydrogel formations	978:1010	the developed hydrogel formations	978:1010	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	5	73	theme	polymer	763:769	arg1	concentration					748:760	concentration	748:760	concentration	748:760	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	5	73	theme	polymer	763:769	arg1	ratios					778:783	polymer mixing ratios	763:783	polymer mixing ratios	763:783	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	7	74	dep	useful	1266:1271	arg1	ii					1259:1260	ii	1259:1260	ii	1259:1260	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	75	from	cells	1345:1349	arg1	hydrogels					1354:1362	hydrogels	1354:1362	hydrogels (fast calcium cross-linking)	1354:1391	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	75	from	cells	1345:1349	arg1	cross-linking					1378:1390	fast calcium cross-linking	1365:1390	fast calcium cross-linking	1365:1390	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	76	theme	fast	1323:1326	arg1	encapsulation					1328:1340	the fast encapsulation	1319:1340	the fast encapsulation of cells in hydrogels (fast calcium cross-linking)	1319:1391	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	3	77	theme	polymer	440:446	arg1	backbone					448:455	the alginate polymer backbone	427:455	the alginate polymer backbone	427:455	We first developed a new synthesis route to introduce alkoxyamine functional groups into the alginate polymer backbone.					
31638775	7	78	from	hydrogels	1354:1362	arg1	provision					1401:1409	the provision	1397:1409	the provision of controlled viscoelastic environments to cultured cells	1397:1467	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	78	from	hydrogels	1354:1362	arg1	encapsulation					1328:1340	the fast encapsulation	1319:1340	the fast encapsulation of cells in hydrogels (fast calcium cross-linking)	1319:1391	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	1	79	theme	design	104:109	arg1	relaxation					77:86	Stress relaxation	70:86	Stress relaxation	70:86	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	1	79	theme	design	104:109	arg1	parameter					111:119	an important design parameter	91:119	an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM)	91:228	Stress relaxation is an important design parameter of biomaterials that can provide an artificial microenvironment mimicking natural extracellular matrix (ECM).					
31638775	6	80	theme	cross-linking	1050:1062	arg1	mechanisms					1064:1073	dual cross-linking mechanisms	1045:1073	dual cross-linking mechanisms with different gelation kinetics	1045:1106	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	3	81	theme	synthesis	363:371	arg1	route					373:377	a new synthesis route	357:377	a new synthesis route to introduce alkoxyamine functional groups into the alginate polymer backbone	357:455	We first developed a new synthesis route to introduce alkoxyamine functional groups into the alginate polymer backbone.					
31638775	4	82	theme	cross-linked	563:574	arg1	hydrogels					585:593	oxime cross-linked alginate hydrogels	557:593	oxime cross-linked alginate hydrogels	557:593	By mixing the resulting polymer (NaAlg-AA) with aldehyde-containing oxidized alginate (NaAlg-Ald), oxime cross-linked alginate hydrogels were prepared.					
31638775	0	83	theme	Tunable	43:49	arg1	Relaxation					58:67	Tunable Stress Relaxation	43:67	Tunable Stress Relaxation	43:67	Oxime Cross-Linked Alginate Hydrogels with Tunable Stress Relaxation.					
31638775	6	84	with	mechanisms	1064:1073	arg1	kinetics					1099:1106	different gelation kinetics	1080:1106	different gelation kinetics	1080:1106	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	7	85	theme	biomedical	1277:1286	arg1	applications					1288:1299	biomedical applications	1277:1299	biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking)	1277:1520	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	6	86	theme	gelation	1090:1097	arg1	kinetics					1099:1106	different gelation kinetics	1080:1106	different gelation kinetics	1080:1106	Combined with the natural capability of the alginate to be cross-linked by divalent cations, the developed hydrogel formations possess the unique capability of dual cross-linking mechanisms with different gelation kinetics.					
31638775	8	87	theme	B-cell	1575:1580	arg1	line					1582:1585	a B-cell line	1573:1585	a B-cell line encapsulated within the developed hydrogel	1573:1628	With biocompatibility confirmed by the culture of a B-cell line encapsulated within the developed hydrogel, this novel hydrogel platform provides a good prospect in various applications where stress relaxation plays a key role in cell-matrix interactions.					
31638775	5	88	theme	NaAlg-Ald	809:817	arg1	oxidation					796:804	oxidation	796:804	oxidation of NaAlg-Ald	796:817	We demonstrate that highly tunable stress relaxation and mechanical properties can be achieved by systematically varying the composition (concentration, polymer mixing ratios, degree of oxidation of NaAlg-Ald) or environmental factors (pH, temperature, and use of catalyst).					
31638775	3	89	theme	alkoxyamine	392:402	arg1	groups					415:420	alkoxyamine functional groups	392:420	alkoxyamine functional groups	392:420	We first developed a new synthesis route to introduce alkoxyamine functional groups into the alginate polymer backbone.					
31638775	2	90	theme	tunable	311:317	arg1	relaxation					326:335	tunable stress relaxation	311:335	tunable stress relaxation	311:335	Here, we report a novel hydrogel platform based on sodium alginate (NaAlg) with tunable stress relaxation.					
31638775	7	91	theme	oxime	1501:1505	arg1	cross-linking					1507:1519	durable oxime cross-linking	1493:1519	durable oxime cross-linking	1493:1519	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
31638775	7	91	theme	oxime	1501:1505	arg1	period					1485:1490	an extended period	1473:1490	an extended period (durable oxime cross-linking)	1473:1520	We demonstrated that this dual cross-linking capability can (i) be utilized for the creation of hydrogels in microbead or microthread geometries and (ii) be useful for biomedical applications that require both the fast encapsulation of cells in hydrogels (fast calcium cross-linking) and the provision of controlled viscoelastic environments to cultured cells for an extended period (durable oxime cross-linking).					
29913270	7	0	used	used	1145:1148	arg2	mAb					1051:1053	A mAb	1049:1053	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD)	1049:1139	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	7	0	used	used	1145:1148	arg2	ingredient					1188:1197	incorporated active pharmaceutical ingredient	1153:1197	incorporated active pharmaceutical ingredient	1153:1197	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	6	1	theme	Solid	902:906	arg1	implants					914:921	Solid lipid implants	902:921	Solid lipid implants (SLIs)	902:928	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	6	1	theme	Solid	902:906	arg1	SLIs					924:927	SLIs	924:927	SLIs	924:927	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	11	2	theme	sufficient	1573:1582	arg1	pressure					1584:1591	sufficient pressure and adequately molten material	1573:1622	pressure	1584:1591	We found that both, sufficient pressure and adequately molten material, is needed to form a suitable implant.					
29913270	6	3	theme	lipid	908:912	arg1	implants					914:921	Solid lipid implants	902:921	Solid lipid implants (SLIs)	902:928	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	6	3	theme	lipid	908:912	arg1	SLIs					924:927	SLIs	924:927	SLIs	924:927	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	7	4	from	available	1055:1063	arg1	matrix					1083:1088	a freeze-dried matrix	1068:1088	a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD)	1068:1139	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	7	5	theme	pharmaceutical	1173:1186	arg1	ingredient					1188:1197	incorporated active pharmaceutical ingredient	1153:1197	incorporated active pharmaceutical ingredient	1153:1197	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	7	5	theme	pharmaceutical	1173:1186	arg1	mAb					1051:1053	A mAb	1049:1053	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD)	1049:1139	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	10	6	theme	monitoring	1474:1483	arg1	tool					1485:1488	the pressure monitoring tool	1461:1488	the pressure monitoring tool	1461:1488	Using the pressure monitoring tool, four characteristic phases were defined for an extrusion run.					
29913270	11	7	theme	molten	1608:1613	arg1	material					1615:1622	sufficient pressure and adequately molten material	1573:1622	material	1615:1622	We found that both, sufficient pressure and adequately molten material, is needed to form a suitable implant.					
29913270	0	8	theme	extruded	74:81	arg1	implants					89:96	extruded lipid implants	74:96	extruded lipid implants	74:96	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	2	9	from	temperature	378:388	arg1	characteristics					430:444	implant characteristics	422:444	implant characteristics	422:444	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	3	10	theme	release	476:482	arg1	characteristics					484:498	release characteristics	476:498	release characteristics	476:498	It is difficult to attribute release characteristics to only one parameter, since the release will always be influenced by a combination of parameters.					
29913270	1	11	theme	protein	212:218	arg1	production					226:235	protein depot production	212:235	protein depot production using a lab-scale twin-screw (tsc)-extruder	212:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	2	12	from	speed	397:401	arg1	characteristics					430:444	implant characteristics	422:444	implant characteristics	422:444	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	12	13	theme	double	1673:1678	arg1	technique					1690:1698	the double extrusion technique	1669:1698	the double extrusion technique	1669:1698	Using the double extrusion technique, release rates could substantially be slowed down without changing formulation.					
29913270	7	14	theme	active	1166:1171	arg1	ingredient					1188:1197	incorporated active pharmaceutical ingredient	1153:1197	incorporated active pharmaceutical ingredient	1153:1197	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	7	14	theme	active	1166:1171	arg1	mAb					1051:1053	A mAb	1049:1053	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD)	1049:1139	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	1	15	theme	depot	220:224	arg1	production					226:235	protein depot production	212:235	protein depot production using a lab-scale twin-screw (tsc)-extruder	212:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	0	16	theme	implants	89:96	arg1	SLE					66:68	SLE	66:68	SLE	66:68	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	0	16	theme	implants	89:96	arg1	extrusion					55:63	solid lipid extrusion	43:63	solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery	43:127	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	8	17	theme	Extrusion	1200:1208	arg1	temperature					1210:1220	Extrusion temperature	1200:1220	Extrusion temperature (33-37 °C)	1200:1231	Extrusion temperature (33-37 °C), screw speed (40-80 rpm) and the lipid composition (30-70% of each triglyceride) were modified.					
29913270	8	17	theme	Extrusion	1200:1208	arg1	33-37 °C					1223:1230	33-37 °C	1223:1230	33-37 °C	1223:1230	Extrusion temperature (33-37 °C), screw speed (40-80 rpm) and the lipid composition (30-70% of each triglyceride) were modified.					
29913270	6	18	theme	melting	975:981	arg1	H12					996:998	the low melting triglyceride H12	967:998	the low melting triglyceride H12	967:998	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	9	19	theme	extruded	1351:1358	arg1	SLIs					1360:1363	freshly extruded SLIs	1343:1363	freshly extruded SLIs	1343:1363	Additionally, freshly extruded SLIs were ground and extruded again as a preparation technique to optimize properties of SLIs.					
29913270	0	20	theme	lipid	83:87	arg1	implants					89:96	extruded lipid implants	74:96	extruded lipid implants	74:96	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	7	21	from	matrix	1083:1088	arg1	available					1055:1063	available	1055:1063	available	1055:1063	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	11	22	theme	suitable	1645:1652	arg1	implant					1654:1660	a suitable implant	1643:1660	a suitable implant	1643:1660	We found that both, sufficient pressure and adequately molten material, is needed to form a suitable implant.					
29913270	1	23	theme	work	146:149	arg1	understanding					163:175	a better understanding	154:175	a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder	154:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	1	23	theme	work	146:149	arg1	aim					134:136	The aim	130:136	The aim of this work	130:149	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	10	24	theme	pressure	1465:1472	arg1	tool					1485:1488	the pressure monitoring tool	1461:1488	the pressure monitoring tool	1461:1488	Using the pressure monitoring tool, four characteristic phases were defined for an extrusion run.					
29913270	4	25	theme	pressure	647:654	arg1	tool					668:671	an online pressure measurement tool	637:671	an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run	637:742	In this study, we describe the use of an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run.					
29913270	5	26	theme	process	798:804	arg1	parameters					806:815	various process parameters	790:815	various process parameters	790:815	We systematically investigated the impact of various process parameters on implant properties as well as release patterns using a monoclonal antibody (mAb).					
29913270	0	27	theme	New	0:2	arg1	insights					4:11	New insights	0:11	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.	0:128	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	7	28	contain	containing	1090:1099	arg2	hydroxypropyl-β-cyclodextrine					1101:1129	hydroxypropyl-β-cyclodextrine	1101:1129	hydroxypropyl-β-cyclodextrine (HP-β-CD)	1101:1139	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	7	28	contain	containing	1090:1099	arg1	matrix					1083:1088	a freeze-dried matrix	1068:1088	a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD)	1068:1139	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	7	28	contain	containing	1090:1099	arg2	HP-β-CD					1132:1138	HP-β-CD	1132:1138	HP-β-CD	1132:1138	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	4	29	theme	online	640:645	arg1	tool					668:671	an online pressure measurement tool	637:671	an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run	637:742	In this study, we describe the use of an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run.					
29913270	5	30	theme	parameters	806:815	arg1	impact					780:785	the impact	776:785	the impact of various process parameters on implant properties as well as release patterns	776:865	We systematically investigated the impact of various process parameters on implant properties as well as release patterns using a monoclonal antibody (mAb).					
29913270	2	31	theme	extrusion	368:376	arg1	temperature					378:388	extrusion temperature	368:388	extrusion temperature	368:388	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	6	32	theme	triglyceride	983:994	arg1	H12					996:998	the low melting triglyceride H12	967:998	the low melting triglyceride H12	967:998	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	0	33	theme	sustained	102:110	arg1	delivery					120:127	sustained protein delivery	102:127	sustained protein delivery	102:127	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	4	34	theme	tool	668:671	arg1	use					630:632	the use	626:632	the use of an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run	626:742	In this study, we describe the use of an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run.					
29913270	1	35	theme	better	156:161	arg1	understanding					163:175	a better understanding	154:175	a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder	154:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	1	35	theme	better	156:161	arg1	aim					134:136	The aim	130:136	The aim of this work	130:149	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	7	36	theme	freeze-dried	1070:1081	arg1	matrix					1083:1088	a freeze-dried matrix	1068:1088	a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD)	1068:1139	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	8	37	theme	triglyceride	1300:1311	arg1	%					1290:1290	30-70%	1285:1290	30-70% of each triglyceride	1285:1311	Extrusion temperature (33-37 °C), screw speed (40-80 rpm) and the lipid composition (30-70% of each triglyceride) were modified.					
29913270	8	37	theme	triglyceride	1300:1311	arg1	triglyceride					1300:1311	each triglyceride	1295:1311	each triglyceride	1295:1311	Extrusion temperature (33-37 °C), screw speed (40-80 rpm) and the lipid composition (30-70% of each triglyceride) were modified.					
29913270	4	38	theme	measurement	656:666	arg1	tool					668:671	an online pressure measurement tool	637:671	an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run	637:742	In this study, we describe the use of an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run.					
29913270	9	39	theme	SLIs	1449:1452	arg1	properties					1435:1444	properties	1435:1444	properties of SLIs	1435:1452	Additionally, freshly extruded SLIs were ground and extruded again as a preparation technique to optimize properties of SLIs.					
29913270	2	40	theme	parameters	349:358	arg1	relationship					325:336	the relationship	321:336	the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics	321:444	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	7	41	theme	available	1055:1063	arg1	ingredient					1188:1197	incorporated active pharmaceutical ingredient	1153:1197	incorporated active pharmaceutical ingredient	1153:1197	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	7	41	theme	available	1055:1063	arg1	mAb					1051:1053	A mAb	1049:1053	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD)	1049:1139	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	1	42	theme	lab-scale	245:253	arg1	-extruder					271:279	a lab-scale twin-screw (tsc)-extruder	243:279	a lab-scale twin-screw (tsc)-extruder	243:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	2	43	from	formulation	407:417	arg1	characteristics					430:444	implant characteristics	422:444	implant characteristics	422:444	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	6	44	theme	low	971:973	arg1	H12					996:998	the low melting triglyceride H12	967:998	the low melting triglyceride H12	967:998	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	2	45	theme	process	341:347	arg1	temperature					378:388	extrusion temperature	368:388	extrusion temperature	368:388	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	2	45	theme	process	341:347	arg1	speed					397:401	screw speed	391:401	screw speed	391:401	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	2	45	theme	process	341:347	arg1	formulation					407:417	formulation	407:417	formulation	407:417	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	2	45	theme	process	341:347	arg1	parameters					349:358	process parameters	341:358	process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics	341:444	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	10	46	theme	extrusion	1538:1546	arg1	run					1548:1550	an extrusion run	1535:1550	an extrusion run	1535:1550	Using the pressure monitoring tool, four characteristic phases were defined for an extrusion run.					
29913270	1	47	theme	twin-screw	255:264	arg1	-extruder					271:279	a lab-scale twin-screw (tsc)-extruder	243:279	a lab-scale twin-screw (tsc)-extruder	243:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	0	48	theme	protein	112:118	arg1	delivery					120:127	sustained protein delivery	102:127	sustained protein delivery	102:127	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	8	49	dep	composition	1272:1282	arg1	%					1290:1290	30-70%	1285:1290	30-70% of each triglyceride	1285:1311	Extrusion temperature (33-37 °C), screw speed (40-80 rpm) and the lipid composition (30-70% of each triglyceride) were modified.					
29913270	8	49	dep	composition	1272:1282	arg1	triglyceride					1300:1311	each triglyceride	1295:1311	each triglyceride	1295:1311	Extrusion temperature (33-37 °C), screw speed (40-80 rpm) and the lipid composition (30-70% of each triglyceride) were modified.					
29913270	0	50	theme	process	18:24	arg1	understanding					26:38	process understanding	18:38	process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery	18:127	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	8	51	theme	screw	1234:1238	arg1	40-80 rpm					1247:1255	40-80 rpm	1247:1255	40-80 rpm	1247:1255	Extrusion temperature (33-37 °C), screw speed (40-80 rpm) and the lipid composition (30-70% of each triglyceride) were modified.					
29913270	8	51	theme	screw	1234:1238	arg1	speed					1240:1244	screw speed	1234:1244	screw speed (40-80 rpm)	1234:1256	Extrusion temperature (33-37 °C), screw speed (40-80 rpm) and the lipid composition (30-70% of each triglyceride) were modified.					
29913270	1	52	theme	solid	180:184	arg1	SLE					203:205	SLE	203:205	SLE	203:205	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	1	52	theme	solid	180:184	arg1	extrusion					192:200	solid lipid extrusion	180:200	solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder	180:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	0	53	theme	lipid	49:53	arg1	SLE					66:68	SLE	66:68	SLE	66:68	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	0	53	theme	lipid	49:53	arg1	extrusion					55:63	solid lipid extrusion	43:63	solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery	43:127	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	6	54	dep	H12	996:998	arg1	D118					1043:1046	D118	1043:1046	D118	1043:1046	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	8	55	theme	lipid	1266:1270	arg1	composition					1272:1282	the lipid composition	1262:1282	the lipid composition (30-70% of each triglyceride)	1262:1312	Extrusion temperature (33-37 °C), screw speed (40-80 rpm) and the lipid composition (30-70% of each triglyceride) were modified.					
29913270	2	56	theme	implant	422:428	arg1	characteristics					430:444	implant characteristics	422:444	implant characteristics	422:444	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	5	57	from	impact	780:785	arg1	properties					828:837	implant properties	820:837	implant properties as well as release patterns	820:865	We systematically investigated the impact of various process parameters on implant properties as well as release patterns using a monoclonal antibody (mAb).					
29913270	5	57	from	impact	780:785	arg1	patterns					858:865	release patterns	850:865	implant properties as well as release patterns	820:865	We systematically investigated the impact of various process parameters on implant properties as well as release patterns using a monoclonal antibody (mAb).					
29913270	0	58	theme	solid	43:47	arg1	SLE					66:68	SLE	66:68	SLE	66:68	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	0	58	theme	solid	43:47	arg1	extrusion					55:63	solid lipid extrusion	43:63	solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery	43:127	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	1	59	theme	tsc	267:269	arg1	-extruder					271:279	a lab-scale twin-screw (tsc)-extruder	243:279	a lab-scale twin-screw (tsc)-extruder	243:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	2	60	theme	screw	391:395	arg1	speed					397:401	screw speed	391:401	screw speed	391:401	In this context, little is known about the relationship of process parameters such as extrusion temperature, screw speed, or formulation on implant characteristics.					
29913270	9	61	theme	preparation	1401:1411	arg1	technique					1413:1421	a preparation technique	1399:1421	a preparation technique to optimize properties of SLIs	1399:1452	Additionally, freshly extruded SLIs were ground and extruded again as a preparation technique to optimize properties of SLIs.					
29913270	4	62	theme	extrusion	730:738	arg1	run					740:742	an extrusion run	727:742	an extrusion run	727:742	In this study, we describe the use of an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run.					
29913270	5	63	theme	release	850:856	arg1	patterns					858:865	release patterns	850:865	implant properties as well as release patterns	820:865	We systematically investigated the impact of various process parameters on implant properties as well as release patterns using a monoclonal antibody (mAb).					
29913270	5	64	theme	monoclonal	875:884	arg1	mAb					896:898	mAb	896:898	mAb	896:898	We systematically investigated the impact of various process parameters on implant properties as well as release patterns using a monoclonal antibody (mAb).					
29913270	5	64	theme	monoclonal	875:884	arg1	antibody					886:893	a monoclonal antibody	873:893	a monoclonal antibody (mAb)	873:899	We systematically investigated the impact of various process parameters on implant properties as well as release patterns using a monoclonal antibody (mAb).					
29913270	1	65	theme	lipid	186:190	arg1	SLE					203:205	SLE	203:205	SLE	203:205	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	1	65	theme	lipid	186:190	arg1	extrusion					192:200	solid lipid extrusion	180:200	solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder	180:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	3	66	theme	parameters	587:596	arg1	combination					572:582	a combination	570:582	a combination of parameters	570:596	It is difficult to attribute release characteristics to only one parameter, since the release will always be influenced by a combination of parameters.					
29913270	4	67	theme	pressure	702:709	arg1	profiles					711:718	pressure profiles	702:718	pressure profiles	702:718	In this study, we describe the use of an online pressure measurement tool which allows to characterize pressure profiles during an extrusion run.					
29913270	6	68	theme	high	1008:1011	arg1	triglyceride					1021:1032	the high melting triglyceride	1004:1032	the high melting triglyceride	1004:1032	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	1	69	theme	extrusion	192:200	arg1	understanding					163:175	a better understanding	154:175	a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder	154:279	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	1	69	theme	extrusion	192:200	arg1	aim					134:136	The aim	130:136	The aim of this work	130:149	The aim of this work is a better understanding of solid lipid extrusion (SLE) for protein depot production using a lab-scale twin-screw (tsc)-extruder.					
29913270	7	70	theme	incorporated	1153:1164	arg1	ingredient					1188:1197	incorporated active pharmaceutical ingredient	1153:1197	incorporated active pharmaceutical ingredient	1153:1197	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	7	70	theme	incorporated	1153:1164	arg1	mAb					1051:1053	A mAb	1049:1053	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD)	1049:1139	A mAb available in a freeze-dried matrix containing hydroxypropyl-β-cyclodextrine (HP-β-CD) was used as incorporated active pharmaceutical ingredient.					
29913270	12	71	theme	extrusion	1680:1688	arg1	technique					1690:1698	the double extrusion technique	1669:1698	the double extrusion technique	1669:1698	Using the double extrusion technique, release rates could substantially be slowed down without changing formulation.					
29913270	6	72	theme	melting	1013:1019	arg1	triglyceride					1021:1032	the high melting triglyceride	1004:1032	the high melting triglyceride	1004:1032	Solid lipid implants (SLIs) were produced by tsc-extrusion using the low melting triglyceride H12 and the high melting triglyceride Dynasan® D118.					
29913270	5	73	theme	various	790:796	arg1	parameters					806:815	various process parameters	790:815	various process parameters	790:815	We systematically investigated the impact of various process parameters on implant properties as well as release patterns using a monoclonal antibody (mAb).					
29913270	0	74	theme	extrusion	55:63	arg1	understanding					26:38	process understanding	18:38	process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery	18:127	New insights into process understanding of solid lipid extrusion (SLE) of extruded lipid implants for sustained protein delivery.					
29913270	10	75	theme	characteristic	1496:1509	arg1	phases					1511:1516	four characteristic phases	1491:1516	four characteristic phases	1491:1516	Using the pressure monitoring tool, four characteristic phases were defined for an extrusion run.					
29913270	12	76	theme	release	1701:1707	arg1	rates					1709:1713	release rates	1701:1713	release rates	1701:1713	Using the double extrusion technique, release rates could substantially be slowed down without changing formulation.					
29913270	5	77	theme	implant	820:826	arg1	properties					828:837	implant properties	820:837	implant properties as well as release patterns	820:865	We systematically investigated the impact of various process parameters on implant properties as well as release patterns using a monoclonal antibody (mAb).					
31507213	2	0	theme	myeloperoxidase	705:719	arg1	levels					690:695	the levels	686:695	the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	686:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	1	1	theme	in	149:150	arg1	activities					169:178	the in vivo anti-inflammatory and in vitro hemostatic activities	115:178	the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract	115:205	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	3	2	theme	same	844:847	arg1	extract					849:855	The same extract	840:855	The same extract	840:855	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	2	3	theme	anti-inflammatory	491:507	arg1	inhibition					509:518	high anti-inflammatory inhibition	486:518	high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw	486:560	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	3	theme	anti-inflammatory	491:507	arg1	%					526:526	63.90%	521:526	63.90%	521:526	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	4	theme	tissue	649:654	arg1	injury					656:661	tissue injury	649:661	tissue injury	649:661	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	5	theme	good	360:363	arg1	them					351:354	them	351:354	them	351:354	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	5	theme	good	360:363	arg1	most					343:346	most	343:346	most	343:346	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	5	theme	good	360:363	arg1	agents					383:388	good anti-inflammatory agents	360:388	good anti-inflammatory agents	360:388	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	6	theme	edema	532:536	arg1	swelling					538:545	edema swelling	532:545	edema swelling	532:545	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	1	7	dep	in	119:120	arg1	vivo					122:125	vivo	122:125	vivo	122:125	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	1	7	dep	in	119:120	arg1	anti-inflammatory					127:143	anti-inflammatory	127:143	anti-inflammatory	127:143	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	2	8	theme	peroxidation	790:801	arg1	malondialdehyde					737:751	malondialdehyde	737:751	malondialdehyde	737:751	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	8	theme	peroxidation	790:801	arg1	product					773:779	the final product	763:779	the final product of lipid peroxidation generated by free radicals (58.58%)	763:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	9	theme	response	636:643	arg1	extent					613:618	both extent	608:618	both extent of inflammatory response and tissue injury	608:661	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	10	theme	Twenty-three	291:302	arg1	metabolites					314:324	Twenty-three secondary metabolites	291:324	Twenty-three secondary metabolites	291:324	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	11	theme	malondialdehyde	737:751	arg1	levels					690:695	the levels	686:695	the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	686:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	1	12	theme	chemical	269:276	arg1	composition					278:288	chemical composition	269:288	chemical composition	269:288	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	2	13	theme	rat	432:434	arg1	assays					446:451	carrageenin-induced rat paw edema assays	412:451	carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	412:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	1	14	theme	hemostatic	158:167	arg1	activities					169:178	the in vivo anti-inflammatory and in vitro hemostatic activities	115:178	the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract	115:205	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	2	15	dep	4 h.	590:593	arg1	prevented					668:676	prevented	668:676	were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	663:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	16	theme	high	486:489	arg1	inhibition					509:518	high anti-inflammatory inhibition	486:518	high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw	486:560	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	16	theme	high	486:489	arg1	%					526:526	63.90%	521:526	63.90%	521:526	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	3	17	theme	flavonoid	991:999	arg1	glycosides					1001:1010	its flavonoid glycosides	987:1010	its flavonoid glycosides	987:1010	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	1	18	dep	in	149:150	arg1	vitro					152:156	vitro	152:156	vitro	152:156	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	0	19	theme	Anti-inflammatory	0:16	arg1	effects					33:39	Anti-inflammatory and hemostatic effects	0:39	Anti-inflammatory and hemostatic effects of Linaria	0:50	Anti-inflammatory and hemostatic effects of Linaria reflexa Desf.					
31507213	3	20	theme	related	976:982	arg1	effect					893:898	a remarkable hemostatic effect	869:898	a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s)	869:973	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	3	20	theme	related	976:982	arg1	content					1012:1018	related to its flavonoid glycosides content	976:1018	related to its flavonoid glycosides content	976:1018	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	1	21	theme	Linaria	183:189	arg1	extract					199:205	Linaria reflexa extract	183:205	Linaria reflexa extract	183:205	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	2	22	theme	rat	554:556	arg1	paw					558:560	the rat paw	550:560	the rat paw	550:560	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	23	theme	injury	656:661	arg1	extent					613:618	both extent	608:618	both extent of inflammatory response and tissue injury	608:661	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	24	theme	inflammatory	623:634	arg1	response					636:643	inflammatory response	623:643	inflammatory response	623:643	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	25	theme	paw	436:438	arg1	assays					446:451	carrageenin-induced rat paw edema assays	412:451	carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	412:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	26	theme	edema	440:444	arg1	assays					446:451	carrageenin-induced rat paw edema assays	412:451	carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	412:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	27	from	inhibition	509:518	arg1	paw					558:560	the rat paw	550:560	the rat paw	550:560	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	3	28	theme	remarkable	871:880	arg1	effect					893:898	a remarkable hemostatic effect	869:898	a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s)	869:973	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	3	28	theme	remarkable	871:880	arg1	content					1012:1018	related to its flavonoid glycosides content	976:1018	related to its flavonoid glycosides content	976:1018	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	1	29	theme	extract	199:205	arg1	activities					169:178	the in vivo anti-inflammatory and in vitro hemostatic activities	115:178	the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract	115:205	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	0	30	theme	Linaria	44:50	arg1	effects					33:39	Anti-inflammatory and hemostatic effects	0:39	Anti-inflammatory and hemostatic effects of Linaria	0:50	Anti-inflammatory and hemostatic effects of Linaria reflexa Desf.					
31507213	3	31	theme	hemostatic	882:891	arg1	effect					893:898	a remarkable hemostatic effect	869:898	a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s)	869:973	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	3	31	theme	hemostatic	882:891	arg1	content					1012:1018	related to its flavonoid glycosides content	976:1018	related to its flavonoid glycosides content	976:1018	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	2	32	theme	carrageenin-induced	412:430	arg1	assays					446:451	carrageenin-induced rat paw edema assays	412:451	carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	412:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	33	theme	swelling	538:545	arg1	inhibition					509:518	high anti-inflammatory inhibition	486:518	high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw	486:560	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	33	theme	swelling	538:545	arg1	%					526:526	63.90%	521:526	63.90%	521:526	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	34	theme	rate	700:703	arg1	myeloperoxidase					705:719	rate myeloperoxidase	700:719	rate myeloperoxidase (60.16%)	700:728	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	34	theme	rate	700:703	arg1	%					727:727	60.16%	722:727	60.16%	722:727	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	35	theme	extract	470:476	arg1	assays					446:451	carrageenin-induced rat paw edema assays	412:451	carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	412:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	36	theme	dose	569:572	arg1	200 mg/kg					574:582	the dose 200 mg/kg	565:582	the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	565:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	3	37	theme	coagulation	929:939	arg1	time					941:944	the coagulation time	925:944	the coagulation time of decalcified plasma (45 s)	925:973	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	2	38	theme	n-butanol	460:468	arg1	extract					470:476	the n-butanol extract	456:476	the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	456:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	39	theme	lipid	784:788	arg1	peroxidation					790:801	lipid peroxidation	784:801	lipid peroxidation generated by free radicals (58.58%)	784:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	40	from	agents	383:388	arg1	line					394:397	line	394:397	line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	394:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	3	41	theme	plasma	961:966	arg1	time					941:944	the coagulation time	925:944	the coagulation time of decalcified plasma (45 s)	925:973	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	1	42	theme	in	119:120	arg1	activities					169:178	the in vivo anti-inflammatory and in vitro hemostatic activities	115:178	the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract	115:205	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	2	43	theme	secondary	304:312	arg1	metabolites					314:324	Twenty-three secondary metabolites	291:324	Twenty-three secondary metabolites	291:324	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	3	44	theme	decalcified	949:959	arg1	45 s					969:972	45 s	969:972	45 s	969:972	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	3	44	theme	decalcified	949:959	arg1	plasma					961:966	decalcified plasma	949:966	decalcified plasma (45 s)	949:973	The same extract showed also a remarkable hemostatic effect established by measuring the coagulation time of decalcified plasma (45 s), related to its flavonoid glycosides content.					
31507213	1	45	theme	reflexa	191:197	arg1	extract					199:205	Linaria reflexa extract	183:205	Linaria reflexa extract	183:205	The work presented here was aimed to investigate the in vivo anti-inflammatory and in vitro hemostatic activities of Linaria reflexa extract and to establish the relationship between its bioactivity and chemical composition.					
31507213	2	46	theme	anti-inflammatory	365:381	arg1	them					351:354	them	351:354	them	351:354	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	46	theme	anti-inflammatory	365:381	arg1	most					343:346	most	343:346	most	343:346	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	46	theme	anti-inflammatory	365:381	arg1	agents					383:388	good anti-inflammatory agents	360:388	good anti-inflammatory agents	360:388	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	47	theme	final	767:771	arg1	malondialdehyde					737:751	malondialdehyde	737:751	malondialdehyde	737:751	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	47	theme	final	767:771	arg1	product					773:779	the final product	763:779	the final product of lipid peroxidation generated by free radicals (58.58%)	763:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	48	with	line	394:397	arg1	data					404:407	data	404:407	data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%)	404:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	0	49	theme	hemostatic	22:31	arg1	effects					33:39	Anti-inflammatory and hemostatic effects	0:39	Anti-inflammatory and hemostatic effects of Linaria	0:50	Anti-inflammatory and hemostatic effects of Linaria reflexa Desf.					
31507213	2	50	theme	free	816:819	arg1	radicals					821:828	free radicals	816:828	free radicals (58.58%)	816:837	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
31507213	2	50	theme	free	816:819	arg1	%					836:836	58.58%	831:836	58.58%	831:836	Twenty-three secondary metabolites were identified, most of them are good anti-inflammatory agents, in line with data by carrageenin-induced rat paw edema assays of the n-butanol extract showing high anti-inflammatory inhibition (63.90%) of edema swelling in the rat paw at the dose 200 mg/kg after 4 h. Furthermore, both extent of inflammatory response and tissue injury were prevented keeping the levels of rate myeloperoxidase (60.16%) and of malondialdehyde, which is the final product of lipid peroxidation generated by free radicals (58.58%).					
30454660	6	0	with	endowed	1114:1120	arg1	properties					1154:1163	different physico-chemical properties	1127:1163	different physico-chemical properties	1127:1163	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	2	1	theme	challenges	369:378	arg1	range					346:350	a range	344:350	a range of environmental challenges and stress	344:389	The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress.					
30454660	0	2	theme	haloplanktis	88:99	arg1	biofilm					35:41	the biofilm	31:41	the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125	31:106	Environmental conditions shape the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.					
30454660	2	3	theme	environmental	355:367	arg1	challenges					369:378	environmental challenges	355:378	environmental challenges	355:378	The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress.					
30454660	6	4	from	present	1267:1273	arg1	matrix					1290:1295	the biofilm matrix	1278:1295	the biofilm matrix	1278:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	6	5	theme	relative	1219:1226	arg1	amount					1228:1233	the relative amount	1215:1233	the relative amount of the different macromolecules present in the biofilm matrix	1215:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	6	5	theme	relative	1219:1226	arg1	macromolecules					1252:1265	the different macromolecules	1238:1265	the different macromolecules present in the biofilm matrix	1238:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	4	6	theme	biofilm	755:761	arg1	typology					763:770	biofilm typology	755:770	biofilm typology	755:770	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	5	7	from	report	964:969	arg1	particular					934:943	particular	934:943	particular	934:943	The characterization was performed on biofilms produced by the bacterium in response to different nutrient abundance and temperatures; in particular, this is the first report describing the structure of a biofilm formed at 0 °C.					
30454660	7	8	theme	biofilm	1483:1489	arg1	formation					1491:1499	the biofilm formation	1479:1499	the biofilm formation in cold environments	1479:1520	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	6	9	from	biofilms	1081:1088	arg1	amount					1103:1108	different amount	1093:1108	different amount	1093:1108	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	6	10	theme	different	1093:1101	arg1	amount					1103:1108	different amount	1093:1108	different amount	1093:1108	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	4	11	theme	organisms	684:692	arg1	organisms					684:692	the model organisms	674:692	the model organisms of cold-adapted bacteria	674:717	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	4	11	theme	organisms	684:692	arg1	haloplanktis					646:657	Pseudoalteromonas haloplanktis TAC125	628:664	Pseudoalteromonas haloplanktis TAC125	628:664	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	4	11	theme	organisms	684:692	arg1	one					667:669	one	667:669	one	667:669	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	6	12	theme	physico-chemical	1137:1152	arg1	properties					1154:1163	different physico-chemical properties	1127:1163	different physico-chemical properties	1127:1163	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	6	13	theme	biofilm	1282:1288	arg1	matrix					1290:1295	the biofilm matrix	1278:1295	the biofilm matrix	1278:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	2	14	from	range	346:350	arg1	protection					328:337	protection	328:337	protection from a range of environmental challenges and stress	328:389	The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress.					
30454660	1	15	theme	life	184:187	arg1	Biofilms					109:116	Biofilms	109:116	Biofilms	109:116	Biofilms are the most widely distributed and successful microbial modes of life.					
30454660	1	15	theme	life	184:187	arg1	modes					175:179	the most widely distributed and successful microbial modes	122:179	the most widely distributed and successful microbial modes of life	122:187	Biofilms are the most widely distributed and successful microbial modes of life.					
30454660	4	16	theme	typology	763:770	arg1	terms					746:750	terms	746:750	terms of biofilm typology and matrix composition	746:793	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	6	17	attach	present	1267:1273	arg2	macromolecules					1252:1265	the different macromolecules	1238:1265	the different macromolecules present in the biofilm matrix	1238:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	6	17	attach	present	1267:1273	arg1	matrix					1290:1295	the biofilm matrix	1278:1295	the biofilm matrix	1278:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	5	18	dep	abundance	903:911	arg1	response					872:879	response	872:879	response	872:879	The characterization was performed on biofilms produced by the bacterium in response to different nutrient abundance and temperatures; in particular, this is the first report describing the structure of a biofilm formed at 0 °C.					
30454660	2	19	from	stability	245:253	arg1	environment					269:279	the growth environment	258:279	the growth environment	258:279	The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress.					
30454660	7	20	theme	different	1334:1342	arg1	structures					1352:1361	different biofilm structures	1334:1361	different biofilm structures	1334:1361	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	0	21	theme	Environmental	0:12	arg1	conditions					14:23	Environmental conditions	0:23	Environmental conditions	0:23	Environmental conditions shape the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.					
30454660	7	22	theme	first	1461:1465	arg1	hints					1467:1471	the first hints	1457:1471	the first hints about the biofilm formation in cold environments	1457:1520	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	6	23	theme	present	1267:1273	arg1	macromolecules					1252:1265	the different macromolecules	1238:1265	the different macromolecules present in the biofilm matrix	1238:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	7	24	dep	changes	1390:1396	arg1	response					1366:1373	response	1366:1373	response	1366:1373	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	0	25	dep	haloplanktis	88:99	arg1	TAC125					101:106	TAC125	101:106	TAC125	101:106	Environmental conditions shape the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.					
30454660	7	26	theme	biofilm	1344:1350	arg1	structures					1352:1361	different biofilm structures	1334:1361	different biofilm structures	1334:1361	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	3	27	theme	environmental	542:554	arg1	adaptation					556:565	their environmental adaptation	536:565	their environmental adaptation	536:565	Bacteria living in cold environments, like Antarctica, can be found as biofilms, even though the mechanisms of how this lifestyle is related to their environmental adaptation have been poorly investigated.					
30454660	5	28	theme	first	958:962	arg1	report					964:969	the first report	954:969	the first report describing the structure of a biofilm formed at 0 °C	954:1022	The characterization was performed on biofilms produced by the bacterium in response to different nutrient abundance and temperatures; in particular, this is the first report describing the structure of a biofilm formed at 0 °C.					
30454660	5	28	theme	first	958:962	arg1	this					946:949	this	946:949	this	946:949	The characterization was performed on biofilms produced by the bacterium in response to different nutrient abundance and temperatures; in particular, this is the first report describing the structure of a biofilm formed at 0 °C.					
30454660	7	29	from	formation	1491:1499	arg1	environments					1509:1520	cold environments	1504:1520	cold environments	1504:1520	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	2	30	theme	nutrients	306:314	arg1	capturing					293:301	the capturing	289:301	the capturing of nutrients	289:314	The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress.					
30454660	1	31	theme	successful	154:163	arg1	Biofilms					109:116	Biofilms	109:116	Biofilms	109:116	Biofilms are the most widely distributed and successful microbial modes of life.					
30454660	1	31	theme	successful	154:163	arg1	modes					175:179	the most widely distributed and successful microbial modes	122:179	the most widely distributed and successful microbial modes of life	122:187	Biofilms are the most widely distributed and successful microbial modes of life.					
30454660	6	32	theme	different	1127:1135	arg1	properties					1154:1163	different physico-chemical properties	1127:1163	different physico-chemical properties	1127:1163	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	6	33	theme	macromolecules	1252:1265	arg1	amount					1228:1233	the relative amount	1215:1233	the relative amount of the different macromolecules present in the biofilm matrix	1215:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	6	33	theme	macromolecules	1252:1265	arg1	macromolecules					1252:1265	the different macromolecules	1238:1265	the different macromolecules present in the biofilm matrix	1238:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	4	34	theme	haloplanktis	646:657	arg1	biofilm					617:623	the biofilm	613:623	the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria,	613:718	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	4	35	theme	model	678:682	arg1	organisms					684:692	the model organisms	674:692	the model organisms of cold-adapted bacteria	674:717	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	6	36	theme	different	1242:1250	arg1	macromolecules					1252:1265	the different macromolecules	1238:1265	the different macromolecules present in the biofilm matrix	1238:1295	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	4	37	theme	Pseudoalteromonas	628:644	arg1	organisms					684:692	the model organisms	674:692	the model organisms of cold-adapted bacteria	674:717	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	4	37	theme	Pseudoalteromonas	628:644	arg1	haloplanktis					646:657	Pseudoalteromonas haloplanktis TAC125	628:664	Pseudoalteromonas haloplanktis TAC125	628:664	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	4	37	theme	Pseudoalteromonas	628:644	arg1	one					667:669	one	667:669	one	667:669	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	3	38	theme	cold	411:414	arg1	environments					416:427	cold environments	411:427	cold environments	411:427	Bacteria living in cold environments, like Antarctica, can be found as biofilms, even though the mechanisms of how this lifestyle is related to their environmental adaptation have been poorly investigated.					
30454660	5	39	theme	different	884:892	arg1	abundance					903:911	different nutrient abundance	884:911	different nutrient abundance	884:911	The characterization was performed on biofilms produced by the bacterium in response to different nutrient abundance and temperatures; in particular, this is the first report describing the structure of a biofilm formed at 0 °C.					
30454660	0	40	theme	bacterium	60:68	arg1	haloplanktis					88:99	the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125	46:106	the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125	46:106	Environmental conditions shape the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.					
30454660	4	41	theme	bacteria	710:717	arg1	organisms					684:692	the model organisms	674:692	the model organisms of cold-adapted bacteria	674:717	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	7	42	theme	cold	1504:1507	arg1	environments					1509:1520	cold environments	1504:1520	cold environments	1504:1520	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	4	43	theme	cold-adapted	697:708	arg1	bacteria					710:717	cold-adapted bacteria	697:717	cold-adapted bacteria	697:717	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	7	44	theme	environment	1378:1388	arg1	changes					1390:1396	environment changes	1378:1396	environment changes	1378:1396	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	4	45	theme	composition	783:793	arg1	terms					746:750	terms	746:750	terms of biofilm typology and matrix composition	746:793	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	1	46	theme	distributed	138:148	arg1	Biofilms					109:116	Biofilms	109:116	Biofilms	109:116	Biofilms are the most widely distributed and successful microbial modes of life.					
30454660	1	46	theme	distributed	138:148	arg1	modes					175:179	the most widely distributed and successful microbial modes	122:179	the most widely distributed and successful microbial modes of life	122:187	Biofilms are the most widely distributed and successful microbial modes of life.					
30454660	7	47	theme	interesting	1415:1425	arg1	strategy					1438:1445	an interesting adaptation strategy	1412:1445	an interesting adaptation strategy	1412:1445	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	7	47	theme	interesting	1415:1425	arg1	capability					1302:1311	The capability	1298:1311	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes	1298:1396	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	7	48	theme	adaptation	1427:1436	arg1	strategy					1438:1445	an interesting adaptation strategy	1412:1445	an interesting adaptation strategy	1412:1445	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	7	48	theme	adaptation	1427:1436	arg1	capability					1302:1311	The capability	1298:1311	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes	1298:1396	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	2	49	theme	bacteria	206:213	arg1	capacity					194:201	The capacity	190:201	The capacity of bacteria to colonize surfaces	190:234	The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress.					
30454660	4	50	theme	matrix	776:781	arg1	composition					783:793	matrix composition	776:793	matrix composition	776:793	In this paper, the biofilm of Pseudoalteromonas haloplanktis TAC125, one of the model organisms of cold-adapted bacteria, has been characterized in terms of biofilm typology and matrix composition.					
30454660	5	51	theme	nutrient	894:901	arg1	abundance					903:911	different nutrient abundance	884:911	different nutrient abundance	884:911	The characterization was performed on biofilms produced by the bacterium in response to different nutrient abundance and temperatures; in particular, this is the first report describing the structure of a biofilm formed at 0 °C.					
30454660	6	52	from	matrix	1290:1295	arg1	present					1267:1273	present	1267:1273	present	1267:1273	The results reported demonstrate that PhTAC125 produces biofilms in different amount and endowed with different physico-chemical properties, like hydrophobicity and roughness, by modulating the relative amount of the different macromolecules present in the biofilm matrix.					
30454660	0	53	theme	Pseudoalteromonas	70:86	arg1	haloplanktis					88:99	the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125	46:106	the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125	46:106	Environmental conditions shape the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.					
30454660	7	54	theme	PhTAC125	1316:1323	arg1	strategy					1438:1445	an interesting adaptation strategy	1412:1445	an interesting adaptation strategy	1412:1445	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	7	54	theme	PhTAC125	1316:1323	arg1	capability					1302:1311	The capability	1298:1311	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes	1298:1396	The capability of PhTAC125 to adopt different biofilm structures in response to environment changes appears to be an interesting adaptation strategy and gives the first hints about the biofilm formation in cold environments.					
30454660	2	55	theme	growth	262:267	arg1	environment					269:279	the growth environment	258:279	the growth environment	258:279	The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress.					
30454660	2	56	theme	stress	384:389	arg1	range					346:350	a range	344:350	a range of environmental challenges and stress	344:389	The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress.					
30454660	0	57	theme	Antarctic	50:58	arg1	haloplanktis					88:99	the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125	46:106	the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125	46:106	Environmental conditions shape the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.					
30454660	5	58	theme	biofilm	1001:1007	arg1	structure					986:994	the structure	982:994	the structure of a biofilm formed at 0 °C	982:1022	The characterization was performed on biofilms produced by the bacterium in response to different nutrient abundance and temperatures; in particular, this is the first report describing the structure of a biofilm formed at 0 °C.					
30454660	1	59	theme	microbial	165:173	arg1	Biofilms					109:116	Biofilms	109:116	Biofilms	109:116	Biofilms are the most widely distributed and successful microbial modes of life.					
30454660	1	59	theme	microbial	165:173	arg1	modes					175:179	the most widely distributed and successful microbial modes	122:179	the most widely distributed and successful microbial modes of life	122:187	Biofilms are the most widely distributed and successful microbial modes of life.					
30600048	2	0	dep	gelatin	485:491	arg1	GM					507:508	GM	507:508	GM	507:508	To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker.					
30600048	2	0	dep	gelatin	485:491	arg1	methacryloyl					493:504	methacryloyl	493:504	gelatin methacryloyl (GM)	485:509	To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker.					
30600048	1	1	theme	crosslinked	177:187	arg1	hydrogel					189:196	a terpolymeric covalently crosslinked hydrogel	151:196	a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA)	151:216	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	1	2	theme	hydrogel	189:196	arg1	synthesis					138:146	synthesis	138:146	synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA)	138:216	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	1	3	theme	cell	276:279	arg1	elasticity					238:247	elasticity	238:247	elasticity	238:247	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	1	3	theme	cell	276:279	arg1	interactions					281:292	cell interactions	276:292	cell interactions	276:292	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	5	4	dep	in	871:872	arg1	vivo					874:877	vivo	874:877	vivo	874:877	In vitro biocompatibility was studied against human chondrocytes, whereas, in vivo biocompatibility and tissue regeneration were confirmed using mouse model.					
30600048	6	5	from	pH	1070:1071	arg1	way					1063:1065	a sustain way	1053:1065	a sustain way at pH 7.4	1053:1075	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	3	6	theme	probable	556:563	arg1	structure					565:573	probable structure	556:573	probable structure	556:573	The composition and probable structure of the terpolymer (HA-g-pHEA-x-GM) were analysed by FTIR, 1H HR-MAS-NMR, and TGA analyses.					
30600048	2	7	theme	2-hydroxyethyl	399:412	arg1	HEA					424:426	HEA	424:426	HEA	424:426	To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker.					
30600048	2	7	theme	2-hydroxyethyl	399:412	arg1	acrylate					414:421	2-hydroxyethyl acrylate	399:421	2-hydroxyethyl acrylate (HEA)	399:427	To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker.					
30600048	6	8	theme	corticosteroid	1016:1029	arg1	drug-dexamethasone					1031:1048	corticosteroid drug-dexamethasone	1016:1048	corticosteroid drug-dexamethasone	1016:1048	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	5	9	theme	mouse	941:945	arg1	model					947:951	mouse model	941:951	mouse model	941:951	In vitro biocompatibility was studied against human chondrocytes, whereas, in vivo biocompatibility and tissue regeneration were confirmed using mouse model.					
30600048	6	10	theme	potential	1222:1230	arg1	applicability					1232:1244	its potential applicability	1218:1244	its potential applicability in cartilage tissue engineering and drug delivery	1218:1294	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	6	11	theme	mechanical	1109:1118	arg1	properties					1120:1129	the tunable mechanical properties	1097:1129	the tunable mechanical properties	1097:1129	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	5	12	theme	In	796:797	arg1	biocompatibility					805:820	In vitro biocompatibility	796:820	In vitro biocompatibility	796:820	In vitro biocompatibility was studied against human chondrocytes, whereas, in vivo biocompatibility and tissue regeneration were confirmed using mouse model.					
30600048	1	13	theme	hyaluronate	201:211	arg1	hydrogel					189:196	a terpolymeric covalently crosslinked hydrogel	151:196	a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA)	151:216	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	0	14	theme	terpolymeric	2:13	arg1	hydrogel					15:22	A terpolymeric hydrogel	0:22	A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial.	0:120	A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial.					
30600048	6	15	theme	serum	997:1001	arg1	albumin					1003:1009	model protein-bovine serum albumin	976:1009	model protein-bovine serum albumin	976:1009	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	5	16	dep	In	796:797	arg1	vitro					799:803	vitro	799:803	vitro	799:803	In vitro biocompatibility was studied against human chondrocytes, whereas, in vivo biocompatibility and tissue regeneration were confirmed using mouse model.					
30600048	3	17	theme	terpolymer	582:591	arg1	composition					540:550	composition	540:550	composition	540:550	The composition and probable structure of the terpolymer (HA-g-pHEA-x-GM) were analysed by FTIR, 1H HR-MAS-NMR, and TGA analyses.					
30600048	3	17	theme	terpolymer	582:591	arg1	structure					565:573	probable structure	556:573	probable structure	556:573	The composition and probable structure of the terpolymer (HA-g-pHEA-x-GM) were analysed by FTIR, 1H HR-MAS-NMR, and TGA analyses.					
30600048	6	18	from	applicability	1232:1244	arg1	engineering					1266:1276	cartilage tissue engineering	1249:1276	cartilage tissue engineering	1249:1276	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	6	18	from	applicability	1232:1244	arg1	delivery					1287:1294	drug delivery	1282:1294	drug delivery	1282:1294	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	4	19	theme	SEM	670:672	arg1	analyses					686:693	The SEM and texture analyses	666:693	The SEM and texture analyses of hydrogel	666:705	The SEM and texture analyses of hydrogel showed interconnected micro-porous network and high mechanical properties, respectively.					
30600048	2	20	theme	cellular	462:469	arg1	interactions					471:482	cellular interactions	462:482	cellular interactions	462:482	To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker.					
30600048	1	21	theme	conventional	298:309	arg1	technique					339:347	conventional free radical polymerization technique	298:347	conventional free radical polymerization technique	298:347	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	6	22	theme	hydrogel	1198:1205	arg1	37 °C.					1081:1086	37 °C. Overall	1081:1094	37 °C. Overall	1081:1094	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	6	22	theme	hydrogel	1198:1205	arg1	properties					1120:1129	the tunable mechanical properties	1097:1129	the tunable mechanical properties	1097:1129	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	6	22	theme	hydrogel	1198:1205	arg1	network					1145:1151	micro-porous network	1132:1151	micro-porous network	1132:1151	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	6	22	theme	hydrogel	1198:1205	arg1	cytocompatibility					1158:1174	cytocompatibility	1158:1174	cytocompatibility	1158:1174	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	5	23	theme	tissue	900:905	arg1	regeneration					907:918	tissue regeneration	900:918	tissue regeneration	900:918	In vitro biocompatibility was studied against human chondrocytes, whereas, in vivo biocompatibility and tissue regeneration were confirmed using mouse model.					
30600048	5	24	theme	human	842:846	arg1	chondrocytes					848:859	human chondrocytes	842:859	human chondrocytes	842:859	In vitro biocompatibility was studied against human chondrocytes, whereas, in vivo biocompatibility and tissue regeneration were confirmed using mouse model.					
30600048	1	25	theme	free	311:314	arg1	technique					339:347	conventional free radical polymerization technique	298:347	conventional free radical polymerization technique	298:347	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	1	26	link	crosslinked	177:187	arg1	hydrogel					189:196	a terpolymeric covalently crosslinked hydrogel	151:196	a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA)	151:216	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	4	27	theme	mechanical	759:768	arg1	properties					770:779	high mechanical properties	754:779	high mechanical properties	754:779	The SEM and texture analyses of hydrogel showed interconnected micro-porous network and high mechanical properties, respectively.					
30600048	6	28	theme	Overall	1088:1094	arg1	37 °C.					1081:1086	37 °C. Overall	1081:1094	37 °C. Overall	1081:1094	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	1	29	theme	radical	316:322	arg1	technique					339:347	conventional free radical polymerization technique	298:347	conventional free radical polymerization technique	298:347	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	4	30	theme	high	754:757	arg1	properties					770:779	high mechanical properties	754:779	high mechanical properties	754:779	The SEM and texture analyses of hydrogel showed interconnected micro-porous network and high mechanical properties, respectively.					
30600048	6	31	theme	tissue	1259:1264	arg1	engineering					1266:1276	cartilage tissue engineering	1249:1276	cartilage tissue engineering	1249:1276	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	4	32	theme	texture	678:684	arg1	analyses					686:693	The SEM and texture analyses	666:693	The SEM and texture analyses of hydrogel	666:705	The SEM and texture analyses of hydrogel showed interconnected micro-porous network and high mechanical properties, respectively.					
30600048	0	33	theme	acrylate-gelatin	52:67	arg1	methacryloyl					69:80	hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl	27:80	hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl	27:80	A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial.					
30600048	1	34	theme	polymerization	324:337	arg1	technique					339:347	conventional free radical polymerization technique	298:347	conventional free radical polymerization technique	298:347	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	3	35	theme	TGA	652:654	arg1	analyses					656:663	TGA analyses	652:663	TGA analyses	652:663	The composition and probable structure of the terpolymer (HA-g-pHEA-x-GM) were analysed by FTIR, 1H HR-MAS-NMR, and TGA analyses.					
30600048	0	36	theme	hyaluronate-hydroxyethyl	27:50	arg1	methacryloyl					69:80	hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl	27:80	hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl	27:80	A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial.					
30600048	6	37	theme	protein-bovine	982:995	arg1	albumin					1003:1009	model protein-bovine serum albumin	976:1009	model protein-bovine serum albumin	976:1009	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	6	38	theme	sustain	1055:1061	arg1	way					1063:1065	a sustain way	1053:1065	a sustain way at pH 7.4	1053:1075	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	5	39	theme	in	871:872	arg1	biocompatibility					879:894	in vivo biocompatibility	871:894	in vivo biocompatibility	871:894	In vitro biocompatibility was studied against human chondrocytes, whereas, in vivo biocompatibility and tissue regeneration were confirmed using mouse model.					
30600048	6	40	theme	model	976:980	arg1	albumin					1003:1009	model protein-bovine serum albumin	976:1009	model protein-bovine serum albumin	976:1009	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	0	41	with	hydrogel	15:22	arg1	properties					95:104	tunable properties	87:104	tunable properties as biomaterial	87:119	A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial.					
30600048	3	42	dep	composition	540:550	arg1	The					536:538	The	536:538	The	536:538	The composition and probable structure of the terpolymer (HA-g-pHEA-x-GM) were analysed by FTIR, 1H HR-MAS-NMR, and TGA analyses.					
30600048	0	43	theme	methacryloyl	69:80	arg1	hydrogel					15:22	A terpolymeric hydrogel	0:22	A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial.	0:120	A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial.					
30600048	1	44	with	biomaterial	221:231	arg1	elasticity					238:247	elasticity	238:247	elasticity	238:247	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	1	44	with	biomaterial	221:231	arg1	properties					261:270	mechanical properties	250:270	mechanical properties	250:270	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	1	44	with	biomaterial	221:231	arg1	interactions					281:292	cell interactions	276:292	cell interactions	276:292	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	6	45	theme	drug	1282:1285	arg1	delivery					1287:1294	drug delivery	1282:1294	drug delivery	1282:1294	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	2	46	theme	mechanical	376:385	arg1	properties					387:396	mechanical properties	376:396	mechanical properties	376:396	To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker.					
30600048	4	47	theme	micro-porous	729:740	arg1	network					742:748	interconnected micro-porous network	714:748	interconnected micro-porous network	714:748	The SEM and texture analyses of hydrogel showed interconnected micro-porous network and high mechanical properties, respectively.					
30600048	6	48	theme	HA-g-pHEA-x-GM	1183:1196	arg1	hydrogel					1198:1205	the HA-g-pHEA-x-GM hydrogel	1179:1205	the HA-g-pHEA-x-GM hydrogel	1179:1205	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	3	49	theme	1H	633:634	arg1	HR-MAS-NMR					636:645	1H HR-MAS-NMR	633:645	1H HR-MAS-NMR	633:645	The composition and probable structure of the terpolymer (HA-g-pHEA-x-GM) were analysed by FTIR, 1H HR-MAS-NMR, and TGA analyses.					
30600048	4	50	theme	interconnected	714:727	arg1	network					742:748	interconnected micro-porous network	714:748	interconnected micro-porous network	714:748	The SEM and texture analyses of hydrogel showed interconnected micro-porous network and high mechanical properties, respectively.					
30600048	2	51	used	used	515:518	arg2	gelatin					485:491	gelatin methacryloyl (GM)	485:509	gelatin methacryloyl (GM)	485:509	To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker.					
30600048	2	51	used	used	515:518	arg2	crosslinker					523:533	crosslinker	523:533	crosslinker	523:533	To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker.					
30600048	2	52	dep	grafted	433:439	arg1	while					448:452	while	448:452	while	448:452	To provide elasticity and mechanical properties, 2-hydroxyethyl acrylate (HEA) was grafted in HA, while to tune cellular interactions, gelatin methacryloyl (GM) was used as crosslinker.					
30600048	1	53	theme	terpolymeric	153:164	arg1	hydrogel					189:196	a terpolymeric covalently crosslinked hydrogel	151:196	a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA)	151:216	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	6	54	theme	tunable	1101:1107	arg1	properties					1120:1129	the tunable mechanical properties	1097:1129	the tunable mechanical properties	1097:1129	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	1	55	theme	mechanical	250:259	arg1	elasticity					238:247	elasticity	238:247	elasticity	238:247	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	1	55	theme	mechanical	250:259	arg1	properties					261:270	mechanical properties	250:270	mechanical properties	250:270	Here, we report synthesis of a terpolymeric covalently crosslinked hydrogel of hyaluronate (HA) as biomaterial with elasticity, mechanical properties and cell interactions via conventional free radical polymerization technique.					
30600048	0	56	theme	tunable	87:93	arg1	properties					95:104	tunable properties	87:104	tunable properties as biomaterial	87:119	A terpolymeric hydrogel of hyaluronate-hydroxyethyl acrylate-gelatin methacryloyl with tunable properties as biomaterial.					
30600048	6	57	theme	cartilage	1249:1257	arg1	engineering					1266:1276	cartilage tissue engineering	1249:1276	cartilage tissue engineering	1249:1276	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	6	58	theme	micro-porous	1132:1143	arg1	network					1145:1151	micro-porous network	1132:1151	micro-porous network	1132:1151	The hydrogel releases model protein-bovine serum albumin, and corticosteroid drug-dexamethasone in a sustain way at pH 7.4 and 37 °C. Overall, the tunable mechanical properties, micro-porous network, and cytocompatibility of the HA-g-pHEA-x-GM hydrogel highlights its potential applicability in cartilage tissue engineering and drug delivery.					
30600048	4	59	theme	hydrogel	698:705	arg1	analyses					686:693	The SEM and texture analyses	666:693	The SEM and texture analyses of hydrogel	666:705	The SEM and texture analyses of hydrogel showed interconnected micro-porous network and high mechanical properties, respectively.					
30590159	2	0	from	analysis	420:427	arg1	effect					436:441	the effect	432:441	the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans	432:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	5	1	theme	GOS	1340:1342	arg1	supplementation					1303:1317	dietary supplementation	1295:1317	dietary supplementation of S. cerevisiae and GOS	1295:1342	The intestinal protease, lipase, and amylase enzyme activities also did not increase significantly between weeks 2 and 4, whereas the values increased significantly after 6 weeks in both groups when fed with dietary supplementation of S. cerevisiae and GOS.					
30590159	4	2	theme	feed	1003:1006	arg1	FCR					1026:1028	FCR	1026:1028	FCR	1026:1028	Similarly, during this period the protein efficiency ratio (PER), feed conversion ratio (FCR) and protein intake (PI) did not increase significantly.					
30590159	4	2	theme	feed	1003:1006	arg1	ratio					1019:1023	feed conversion ratio	1003:1023	feed conversion ratio (FCR)	1003:1029	Similarly, during this period the protein efficiency ratio (PER), feed conversion ratio (FCR) and protein intake (PI) did not increase significantly.					
30590159	5	3	theme	enzyme	1132:1137	arg1	activities					1139:1148	amylase enzyme activities	1124:1148	amylase enzyme activities	1124:1148	The intestinal protease, lipase, and amylase enzyme activities also did not increase significantly between weeks 2 and 4, whereas the values increased significantly after 6 weeks in both groups when fed with dietary supplementation of S. cerevisiae and GOS.					
30590159	3	4	dep	weeks	926:930	arg1	6-8					932:934	6-8	932:934	6-8	932:934	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	5	5	theme	cerevisiae	1325:1334	arg1	supplementation					1303:1317	dietary supplementation	1295:1317	dietary supplementation of S. cerevisiae and GOS	1295:1342	The intestinal protease, lipase, and amylase enzyme activities also did not increase significantly between weeks 2 and 4, whereas the values increased significantly after 6 weeks in both groups when fed with dietary supplementation of S. cerevisiae and GOS.					
30590159	6	6	theme	non-infected	1426:1437	arg1	fish					1439:1442	infected and non-infected fish	1413:1442	infected and non-infected fish fed with S. cerevisiae and GOS diets	1413:1479	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	3	7	theme	2.5 g kg-1	736:745	arg1	diet					747:750	feeding 2.5 g kg-1 diet	728:750	feeding 2.5 g kg-1 diet	728:750	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	9	8	theme	non-infected	2224:2235	arg1	fish					2237:2240	the non-infected fish	2220:2240	the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8	2220:2310	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	6	9	theme	infected	1413:1420	arg1	fish					1439:1442	infected and non-infected fish	1413:1442	infected and non-infected fish fed with S. cerevisiae and GOS diets	1413:1479	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	2	10	from	growth	479:484	arg1	punctatus					567:575	Channa punctatus	560:575	Channa punctatus against Aphanomyces invadans	560:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	3	11	from	increase	886:893	arg1	MWG					898:900	MWG	898:900	MWG	898:900	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	3	12	dep	groups	866:871	arg1	both					836:839	both	836:839	both	836:839	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	1	13	used	used	196:199	arg2	prebiotics					168:177	prebiotics	168:177	prebiotics	168:177	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30590159	1	13	used	used	196:199	arg2	probiotics					153:162	probiotics	153:162	probiotics	153:162	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30590159	1	14	theme	human	135:139	arg1	care					148:151	human health care	135:151	human health care	135:151	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30590159	2	15	theme	Channa	560:565	arg1	punctatus					567:575	Channa punctatus	560:575	Channa punctatus against Aphanomyces invadans	560:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	2	16	from	activity	505:512	arg1	punctatus					567:575	Channa punctatus	560:575	Channa punctatus against Aphanomyces invadans	560:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	10	17	dep	fish	2349:2352	arg1	diets					2394:2398	enriched diets	2385:2398	enriched diets	2385:2398	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	2	18	theme	enzymes	497:503	arg1	activity					505:512	digestive enzymes activity	487:512	digestive enzymes activity	487:512	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	10	19	theme	infected	2470:2477	arg1	fish					2479:2482	the infected fish	2466:2482	the infected fish fed with S. cerevisiae and GOS diets	2466:2519	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	3	20	theme	weight	793:798	arg1	MWG					806:808	MWG	806:808	MWG	806:808	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	3	20	theme	weight	793:798	arg1	gain					800:803	the mean weight gain	784:803	the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups	784:871	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	0	21	from	effect	30:35	arg1	punctatus					76:84	Channa punctatus	69:84	Channa punctatus	69:84	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.					
30590159	1	22	theme	immune	353:358	arg1	system					360:365	the host immune system	344:365	the host immune system	344:365	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30590159	8	23	theme	containing	2084:2093	arg1	diets					2095:2099	containing diets	2084:2099	containing diets after 6 weeks	2084:2113	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	10	24	located	observed	2454:2461	arg2	mortality					2440:2448	15% and 10% mortality	2428:2448	15% and 10% mortality	2428:2448	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	10	24	located	observed	2454:2461	arg1	fish					2479:2482	the infected fish	2466:2482	the infected fish fed with S. cerevisiae and GOS diets	2466:2519	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	7	25	theme	peroxidase	1808:1817	arg1	activities					1825:1834	the catalase (CAT) and glutathione peroxidase (GPx) activities	1773:1834	the catalase (CAT) and glutathione peroxidase (GPx) activities	1773:1834	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	7	26	theme	dismutase	1572:1580	arg1	activity					1590:1597	The total superoxide dismutase (t-SOD) activity	1551:1597	The total superoxide dismutase (t-SOD) activity	1551:1597	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	7	27	theme	total	1555:1559	arg1	t-SOD					1583:1587	t-SOD	1583:1587	t-SOD	1583:1587	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	7	27	theme	total	1555:1559	arg1	dismutase					1572:1580	The total superoxide dismutase	1551:1580	The total superoxide dismutase (t-SOD) activity	1551:1597	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	8	28	theme	lysozyme	1920:1927	arg1	activities					1980:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	0	29	theme	Comparative	0:10	arg1	effect					30:35	Comparative immunostimulatory effect	0:35	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.	0:114	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.					
30590159	5	30	theme	intestinal	1091:1100	arg1	protease					1102:1109	The intestinal protease	1087:1109	The intestinal protease	1087:1109	The intestinal protease, lipase, and amylase enzyme activities also did not increase significantly between weeks 2 and 4, whereas the values increased significantly after 6 weeks in both groups when fed with dietary supplementation of S. cerevisiae and GOS.					
30590159	11	31	theme	microbiota	2738:2747	arg1	composition					2749:2759	gut microbiota composition	2734:2759	gut microbiota composition	2734:2759	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	6	32	theme	TB	1508:1509	arg1	count					1512:1516	the total bacterial (TB) count	1487:1516	the total bacterial (TB) count	1487:1516	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	8	33	theme	acid	1930:1933	arg1	ACP					1948:1950	ACP	1948:1950	ACP	1948:1950	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	8	33	theme	acid	1930:1933	arg1	phosphatase					1935:1945	acid phosphatase	1930:1945	acid phosphatase (ACP)	1930:1951	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	11	34	theme	dietary	2614:2620	arg1	supplementation					2622:2636	dietary supplementation	2614:2636	dietary supplementation of GOS diet	2614:2648	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	8	35	theme	innate	1880:1885	arg1	activities					1980:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	8	35	theme	innate	1880:1885	arg1	parameters					1894:1903	The innate immune parameters	1876:1903	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1876:1989	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	1	36	theme	disease	298:304	arg1	resistance					306:315	disease resistance	298:315	disease resistance	298:315	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30590159	7	37	theme	catalase	1777:1784	arg1	activities					1825:1834	the catalase (CAT) and glutathione peroxidase (GPx) activities	1773:1834	the catalase (CAT) and glutathione peroxidase (GPx) activities	1773:1834	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	9	38	contain	containing	2273:2282	arg2	diets					2284:2288	diets	2284:2288	diets between weeks 6 and 8	2284:2310	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	9	38	contain	containing	2273:2282	arg1	GOS					2269:2271	GOS	2269:2271	GOS containing diets between weeks 6 and 8	2269:2310	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	9	39	theme	nitric	2138:2143	arg1	NO					2152:2153	NO	2152:2153	NO	2152:2153	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	9	39	theme	nitric	2138:2143	arg1	oxide					2145:2149	the plasma nitric oxide	2127:2149	the plasma nitric oxide (NO) level	2127:2160	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	10	40	theme	%	2430:2430	arg1	mortality					2440:2448	15% and 10% mortality	2428:2448	15% and 10% mortality	2428:2448	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	2	41	theme	comparative	408:418	arg1	analysis					420:427	a comparative analysis	406:427	a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans	406:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	0	42	theme	probiotics	40:49	arg1	effect					30:35	Comparative immunostimulatory effect	0:35	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.	0:114	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.					
30590159	7	43	theme	supplementation	1721:1735	arg1	diets					1737:1741	GOS supplementation diets	1717:1741	GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities	1717:1834	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	4	44	theme	protein	971:977	arg1	ratio					990:994	the protein efficiency ratio	967:994	the protein efficiency ratio (PER)	967:1000	Similarly, during this period the protein efficiency ratio (PER), feed conversion ratio (FCR) and protein intake (PI) did not increase significantly.					
30590159	0	45	theme	prebiotics	55:64	arg1	effect					30:35	Comparative immunostimulatory effect	0:35	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.	0:114	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.					
30590159	10	46	theme	GOS	2511:2513	arg1	diets					2515:2519	GOS diets	2511:2519	GOS diets	2511:2519	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	11	47	theme	growth	2685:2690	arg1	performance					2692:2702	better growth performance	2678:2702	better growth performance	2678:2702	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	10	48	theme	10	2436:2437	arg1	%					2438:2438	%	2438:2438	%	2438:2438	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	1	49	theme	beneficial	242:251	arg1	microbiota					257:266	beneficial gut microbiota	242:266	beneficial gut microbiota	242:266	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30590159	9	50	theme	protein	2172:2178	arg1	content					2185:2191	total protein (TP) content	2166:2191	total protein (TP) content	2166:2191	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	7	51	theme	non-infected	1672:1683	arg1	fish					1685:1688	the non-infected fish	1668:1688	the non-infected fish fed with S. cerevisiae	1668:1711	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	4	52	theme	conversion	1008:1017	arg1	FCR					1026:1028	FCR	1026:1028	FCR	1026:1028	Similarly, during this period the protein efficiency ratio (PER), feed conversion ratio (FCR) and protein intake (PI) did not increase significantly.					
30590159	4	52	theme	conversion	1008:1017	arg1	ratio					1019:1023	feed conversion ratio	1003:1023	feed conversion ratio (FCR)	1003:1029	Similarly, during this period the protein efficiency ratio (PER), feed conversion ratio (FCR) and protein intake (PI) did not increase significantly.					
30590159	9	53	theme	TP	2181:2182	arg1	content					2185:2191	total protein (TP) content	2166:2191	total protein (TP) content	2166:2191	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	6	54	theme	S.	1355:1356	arg1	count					1369:1373	The total S. cerevisiae count	1345:1373	The total S. cerevisiae count	1345:1373	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	5	55	theme	amylase	1124:1130	arg1	activities					1139:1148	amylase enzyme activities	1124:1148	amylase enzyme activities	1124:1148	The intestinal protease, lipase, and amylase enzyme activities also did not increase significantly between weeks 2 and 4, whereas the values increased significantly after 6 weeks in both groups when fed with dietary supplementation of S. cerevisiae and GOS.					
30590159	2	56	theme	Aphanomyces	585:595	arg1	invadans					597:604	Aphanomyces invadans	585:604	Aphanomyces invadans	585:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	3	57	theme	feeding	728:734	arg1	diet					747:750	feeding 2.5 g kg-1 diet	728:750	feeding 2.5 g kg-1 diet	728:750	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	8	58	theme	infected	2022:2029	arg1	fish					2048:2051	the infected and non-infected fish	2018:2051	the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks	2018:2113	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	4	59	dep	ratio	990:994	arg1	PER					997:999	PER	997:999	PER	997:999	Similarly, during this period the protein efficiency ratio (PER), feed conversion ratio (FCR) and protein intake (PI) did not increase significantly.					
30590159	6	60	theme	fish	1439:1442	arg1	gut					1406:1408	the gut	1402:1408	the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets	1402:1479	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	5	61	dep	increase	1163:1170	arg1	whereas					1209:1215	whereas	1209:1215	whereas	1209:1215	The intestinal protease, lipase, and amylase enzyme activities also did not increase significantly between weeks 2 and 4, whereas the values increased significantly after 6 weeks in both groups when fed with dietary supplementation of S. cerevisiae and GOS.					
30590159	8	62	theme	non-infected	2035:2046	arg1	fish					2048:2051	the infected and non-infected fish	2018:2051	the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks	2018:2113	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	4	63	theme	protein	1035:1041	arg1	intake					1043:1048	protein intake	1035:1048	protein intake (PI)	1035:1053	Similarly, during this period the protein efficiency ratio (PER), feed conversion ratio (FCR) and protein intake (PI) did not increase significantly.					
30590159	4	63	theme	protein	1035:1041	arg1	PI					1051:1052	PI	1051:1052	PI	1051:1052	Similarly, during this period the protein efficiency ratio (PER), feed conversion ratio (FCR) and protein intake (PI) did not increase significantly.					
30590159	11	64	theme	immune	2766:2771	arg1	response					2773:2780	immune response	2766:2780	immune response	2766:2780	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	2	65	theme	immune	541:546	arg1	response					548:555	immune response	541:555	immune response	541:555	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	1	66	theme	important	214:222	arg1	role					224:227	their important role	208:227	their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system	208:365	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30590159	11	67	theme	cerevisiae	2798:2807	arg1	diet					2809:2812	S. cerevisiae diet	2795:2812	S. cerevisiae diet	2795:2812	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	3	68	theme	mean	788:791	arg1	MWG					806:808	MWG	806:808	MWG	806:808	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	3	68	theme	mean	788:791	arg1	gain					800:803	the mean weight gain	784:803	the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups	784:871	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	6	69	theme	total	1491:1495	arg1	count					1512:1516	the total bacterial (TB) count	1487:1516	the total bacterial (TB) count	1487:1516	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	11	70	theme	digestive	2705:2713	arg1	activities					2722:2731	digestive enzyme activities	2705:2731	digestive enzyme activities	2705:2731	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	1	71	theme	health	141:146	arg1	care					148:151	human health care	135:151	human health care	135:151	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30590159	2	72	from	activity	527:534	arg1	punctatus					567:575	Channa punctatus	560:575	Channa punctatus against Aphanomyces invadans	560:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	5	73	from	weeks	1260:1264	arg1	groups					1274:1279	both groups	1269:1279	both groups	1269:1279	The intestinal protease, lipase, and amylase enzyme activities also did not increase significantly between weeks 2 and 4, whereas the values increased significantly after 6 weeks in both groups when fed with dietary supplementation of S. cerevisiae and GOS.					
30590159	6	74	theme	GOS	1471:1473	arg1	diets					1475:1479	GOS diets	1471:1479	GOS diets	1471:1479	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	7	75	theme	malonaldehyde	1607:1619	arg1	concentration					1627:1639	the malonaldehyde (MDA) concentration	1603:1639	the malonaldehyde (MDA) concentration	1603:1639	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	2	76	theme	digestive	487:495	arg1	enzymes					497:503	digestive enzymes	487:503	digestive enzymes activity	487:512	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	2	77	from	effect	436:441	arg1	growth					479:484	growth	479:484	growth	479:484	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	2	77	from	effect	436:441	arg1	response					548:555	immune response	541:555	immune response	541:555	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	2	77	from	effect	436:441	arg1	activity					505:512	digestive enzymes activity	487:512	digestive enzymes activity	487:512	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	2	77	from	effect	436:441	arg1	activity					527:534	antioxidant activity	515:534	antioxidant activity	515:534	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	0	78	theme	Aphanomyces	94:104	arg1	invadans					106:113	Aphanomyces invadans	94:113	Aphanomyces invadans	94:113	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.					
30590159	1	79	theme	host	348:351	arg1	system					360:365	the host immune system	344:365	the host immune system	344:365	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30590159	3	80	theme	control	858:864	arg1	groups					866:871	both the infected and control groups	836:871	groups	866:871	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	10	81	theme	non-infected	2336:2347	arg1	fish					2349:2352	the non-infected fish	2332:2352	the non-infected fish fed with S. cerevisiae	2332:2375	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	11	82	theme	enzyme	2715:2720	arg1	activities					2722:2731	digestive enzyme activities	2705:2731	digestive enzyme activities	2705:2731	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	10	83	theme	%	2438:2438	arg1	mortality					2440:2448	15% and 10% mortality	2428:2448	15% and 10% mortality	2428:2448	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	7	84	theme	glutathione	1796:1806	arg1	peroxidase					1808:1817	glutathione peroxidase	1796:1817	glutathione peroxidase (GPx)	1796:1823	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	7	84	theme	glutathione	1796:1806	arg1	GPx					1820:1822	GPx	1820:1822	GPx	1820:1822	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	3	85	theme	infected	845:852	arg1	groups					866:871	both the infected and control groups	836:871	groups	866:871	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	2	86	theme	antioxidant	515:525	arg1	activity					527:534	antioxidant activity	515:534	antioxidant activity	515:534	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	2	87	theme	first	395:399	arg1	time					401:404	the first time	391:404	the first time	391:404	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	0	88	theme	immunostimulatory	12:28	arg1	effect					30:35	Comparative immunostimulatory effect	0:35	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.	0:114	Comparative immunostimulatory effect of probiotics and prebiotics in Channa punctatus against Aphanomyces invadans.					
30590159	11	89	theme	gut	2734:2736	arg1	composition					2749:2759	gut microbiota composition	2734:2759	gut microbiota composition	2734:2759	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	7	90	theme	superoxide	1561:1570	arg1	t-SOD					1583:1587	t-SOD	1583:1587	t-SOD	1583:1587	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	7	90	theme	superoxide	1561:1570	arg1	dismutase					1572:1580	The total superoxide dismutase	1551:1580	The total superoxide dismutase (t-SOD) activity	1551:1597	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	8	91	theme	phosphatase	1935:1945	arg1	activities					1980:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	4	92	theme	efficiency	979:988	arg1	ratio					990:994	the protein efficiency ratio	967:994	the protein efficiency ratio (PER)	967:1000	Similarly, during this period the protein efficiency ratio (PER), feed conversion ratio (FCR) and protein intake (PI) did not increase significantly.					
30590159	11	93	theme	GOS	2641:2643	arg1	diet					2645:2648	GOS diet	2641:2648	GOS diet	2641:2648	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	8	94	theme	immune	1887:1892	arg1	activities					1980:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	8	94	theme	immune	1887:1892	arg1	parameters					1894:1903	The innate immune parameters	1876:1903	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1876:1989	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	10	95	theme	enriched	2385:2392	arg1	diets					2394:2398	enriched diets	2385:2398	enriched diets	2385:2398	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	9	96	theme	plasma	2131:2136	arg1	NO					2152:2153	NO	2152:2153	NO	2152:2153	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	9	96	theme	plasma	2131:2136	arg1	oxide					2145:2149	the plasma nitric oxide	2127:2149	the plasma nitric oxide (NO) level	2127:2160	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	11	97	theme	diet	2645:2648	arg1	supplementation					2622:2636	dietary supplementation	2614:2636	dietary supplementation of GOS diet	2614:2648	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	3	98	from	weeks	926:930	arg1	significant					909:919	significant	909:919	significant	909:919	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	2	99	theme	prebiotics	465:474	arg1	effect					436:441	the effect	432:441	the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans	432:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	10	100	theme	15	2428:2429	arg1	%					2430:2430	%	2430:2430	%	2430:2430	In the control and the non-infected fish fed with S. cerevisiae and GOS enriched diets caused no mortality whereas 15% and 10% mortality was observed in the infected fish fed with S. cerevisiae and GOS diets, respectively.					
30590159	9	101	theme	oxide	2145:2149	arg1	level					2156:2160	the plasma nitric oxide (NO) level	2127:2160	the plasma nitric oxide (NO) level	2127:2160	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	8	102	theme	plasma	1913:1918	arg1	lysozyme					1920:1927	plasma lysozyme	1913:1927	plasma lysozyme	1913:1927	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	6	103	dep	S.	1355:1356	arg1	cerevisiae					1358:1367	cerevisiae	1358:1367	cerevisiae	1358:1367	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	8	104	theme	myeloperoxidase	1958:1972	arg1	activities					1980:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities	1913:1989	The innate immune parameters such as plasma lysozyme, acid phosphatase (ACP), and myeloperoxidase (MPO) activities increased significantly in the infected and non-infected fish fed with S. cerevisiae and GOS containing diets after 6 weeks.					
30590159	2	105	theme	probiotics	450:459	arg1	effect					436:441	the effect	432:441	the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans	432:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	3	106	from	gain	800:803	arg1	groups					866:871	both the infected and control groups	836:871	groups	866:871	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	5	107	theme	dietary	1295:1301	arg1	supplementation					1303:1317	dietary supplementation	1295:1317	dietary supplementation of S. cerevisiae and GOS	1295:1342	The intestinal protease, lipase, and amylase enzyme activities also did not increase significantly between weeks 2 and 4, whereas the values increased significantly after 6 weeks in both groups when fed with dietary supplementation of S. cerevisiae and GOS.					
30590159	11	108	theme	better	2678:2683	arg1	performance					2692:2702	better growth performance	2678:2702	better growth performance	2678:2702	This study indicates that the infected and non-infected C. punctatus fed with dietary supplementation of GOS diet at 2.5 g kg-1 had exhibited better growth performance, digestive enzyme activities, gut microbiota composition, and immune response than that of S. cerevisiae diet.					
30590159	7	109	theme	GOS	1717:1719	arg1	diets					1737:1741	GOS supplementation diets	1717:1741	GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities	1717:1834	The total superoxide dismutase (t-SOD) activity and the malonaldehyde (MDA) concentration increased significantly in the non-infected fish fed with S. cerevisiae and GOS supplementation diets between weeks 6 and 8 whereas the catalase (CAT) and glutathione peroxidase (GPx) activities increased significantly only on week 8.					
30590159	6	110	theme	bacterial	1497:1505	arg1	count					1512:1516	the total bacterial (TB) count	1487:1516	the total bacterial (TB) count	1487:1516	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	9	111	theme	total	2166:2170	arg1	content					2185:2191	total protein (TP) content	2166:2191	total protein (TP) content	2166:2191	Similarly, the plasma nitric oxide (NO) level and total protein (TP) content significantly increased in the non-infected fish fed with S. cerevisiae and GOS containing diets between weeks 6 and 8.					
30590159	3	112	dep	cerevisiae	651:660	arg1	cerevisiae					666:675	S. cerevisiae	663:675	S. cerevisiae	663:675	Among the diets enriched with Saccharomyces cerevisiae (S. cerevisiae) and Galactooligosaccharide (GOS) in C. punctatus, feeding 2.5 g kg-1 diet did not significantly influence the mean weight gain (MWG) between weeks 2 and 4 in both the infected and control groups; however the increase in MWG became significant from weeks 6-8.					
30590159	2	113	from	response	548:555	arg1	punctatus					567:575	Channa punctatus	560:575	Channa punctatus against Aphanomyces invadans	560:604	This study reports for the first time a comparative analysis on the effect of the probiotics and prebiotics on growth, digestive enzymes activity, antioxidant activity, and immune response in Channa punctatus against Aphanomyces invadans.					
30590159	6	114	theme	total	1349:1353	arg1	count					1369:1373	The total S. cerevisiae count	1345:1373	The total S. cerevisiae count	1345:1373	The total S. cerevisiae count significantly increased in the gut of infected and non-infected fish fed with S. cerevisiae and GOS diets while the total bacterial (TB) count decreased between weeks 6 and 8.					
30590159	1	115	theme	gut	253:255	arg1	microbiota					257:266	beneficial gut microbiota	242:266	beneficial gut microbiota	242:266	In aquaculture and human health care probiotics and prebiotics have been widely used due to their important role in enhancing beneficial gut microbiota, promoting growth, increasing disease resistance, and positively modulating the host immune system.					
30216664	4	0	theme	drug	846:849	arg1	kinetics					859:866	drug release kinetics	846:866	drug release kinetics	846:866	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	2	1	theme	thickness	545:553	arg1	terms					498:502	terms	498:502	terms of suspension stability, homogeneity and thickness of coatings	498:565	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	2	2	theme	coatings	558:565	arg1	homogeneity					529:539	homogeneity	529:539	homogeneity	529:539	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	2	2	theme	coatings	558:565	arg1	stability					518:526	suspension stability	507:526	suspension stability	507:526	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	2	2	theme	coatings	558:565	arg1	thickness					545:553	thickness	545:553	thickness	545:553	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	9	3	theme	close	1588:1592	arg1	interaction					1594:1604	close interaction	1588:1604	close interaction between the coating surface and bone	1588:1641	Moreover, the developed coatings formed apatite-like crystals upon immersion in simulated body fluid, indicating the possibility of achieving close interaction between the coating surface and bone.					
30216664	3	4	theme	PEEK/BG	696:702	arg1	layers					704:709	PEEK/BG layers	696:709	PEEK/BG layers	696:709	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	1	5	theme	electrophoretic	246:260	arg1	EPD					274:276	EPD	274:276	EPD	274:276	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	1	5	theme	electrophoretic	246:260	arg1	deposition					262:271	electrophoretic deposition	246:271	electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel)	246:373	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	4	6	theme	in	824:825	arg1	bioactivity					833:843	in vitro bioactivity	824:843	in vitro bioactivity	824:843	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	4	7	theme	bioactivity	833:843	arg1	terms					764:768	terms	764:768	terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity	764:893	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	9	8	theme	developed	1460:1468	arg1	coatings					1470:1477	the developed coatings	1456:1477	the developed coatings	1456:1477	Moreover, the developed coatings formed apatite-like crystals upon immersion in simulated body fluid, indicating the possibility of achieving close interaction between the coating surface and bone.					
30216664	2	9	theme	stability	518:526	arg1	terms					498:502	terms	498:502	terms of suspension stability, homogeneity and thickness of coatings	498:565	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	1	10	theme	chitosan/bioactive	181:198	arg1	BG					207:208	BG	207:208	BG	207:208	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	1	10	theme	chitosan/bioactive	181:198	arg1	glass					200:204	chitosan/bioactive glass	181:204	chitosan/bioactive glass (BG)/lawsone coatings	181:226	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	1	11	theme	stainless	358:366	arg1	steel					368:372	316-L stainless steel	352:372	316-L stainless steel	352:372	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	0	12	theme	lawsone	30:36	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of lawsone	0:36	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	4	13	theme	antibacterial	872:884	arg1	activity					886:893	antibacterial activity	872:893	antibacterial activity	872:893	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	0	14	theme	antibacterial	129:141	arg1	coating					157:163	antibacterial and bioactive coating	129:163	antibacterial and bioactive coating	129:163	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	4	15	theme	kinetics	859:866	arg1	terms					764:768	terms	764:768	terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity	764:893	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	2	16	theme	homogeneity	529:539	arg1	terms					498:502	terms	498:502	terms of suspension stability, homogeneity and thickness of coatings	498:565	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	8	17	theme	top	1372:1374	arg1	layer					1376:1380	the top layer	1368:1380	the top layer	1368:1380	The presence of lawsone in the top layer provided antibacterial effects against Staphylococcus carnosus.					
30216664	0	18	theme	bioactive	147:155	arg1	coating					157:163	antibacterial and bioactive coating	129:163	antibacterial and bioactive coating	129:163	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	3	19	theme	EPD	596:598	arg1	parameters					600:609	the optimized EPD parameters	582:609	the optimized EPD parameters	582:609	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	9	20	theme	simulated	1526:1534	arg1	fluid					1541:1545	simulated body fluid	1526:1545	simulated body fluid	1526:1545	Moreover, the developed coatings formed apatite-like crystals upon immersion in simulated body fluid, indicating the possibility of achieving close interaction between the coating surface and bone.					
30216664	2	21	from	steel	489:493	arg1	terms					498:502	terms	498:502	terms of suspension stability, homogeneity and thickness of coatings	498:565	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	0	22	theme	BG	62:63	arg1	composite					75:83	bioactive glass (BG)/chitosan composite	45:83	bioactive glass (BG)/chitosan composite	45:83	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	2	23	theme	chitosan/BG/lawsone	442:460	arg1	EPD					435:437	the EPD	431:437	the EPD of chitosan/BG/lawsone	431:460	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	0	24	theme	/chitosan	65:73	arg1	composite					75:83	bioactive glass (BG)/chitosan composite	45:83	bioactive glass (BG)/chitosan composite	45:83	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	2	25	gly	homogeneity	529:539	arg1	coatings					558:565	coatings	558:565	coatings	558:565	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	2	26	theme	stainless	479:487	arg1	steel					489:493	stainless steel	479:493	stainless steel in terms of suspension stability, homogeneity and thickness of coatings	479:565	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	7	27	theme	corrosion	1191:1199	arg1	resistance					1201:1210	higher corrosion resistance	1184:1210	higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel	1184:1273	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	7	27	theme	corrosion	1191:1199	arg1	times					1216:1220	10 times	1213:1220	10 times	1213:1220	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	6	28	theme	Electron	1075:1082	arg1	SEM					1096:1098	SEM	1096:1098	SEM	1096:1098	Moreover, the deposition of chitosan/BG coatings was confirmed by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR).					
30216664	6	28	theme	Electron	1075:1082	arg1	Microscopy					1084:1093	Scanning Electron Microscopy	1066:1093	Scanning Electron Microscopy (SEM)	1066:1099	Moreover, the deposition of chitosan/BG coatings was confirmed by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR).					
30216664	0	29	theme	Electrophoretic	0:14	arg1	deposition					16:25	Electrophoretic deposition	0:25	Electrophoretic deposition of lawsone	0:36	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	7	30	theme	initial	1313:1319	arg1	attachment					1329:1338	initial protein attachment	1313:1338	initial protein attachment	1313:1338	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	4	31	theme	layered	725:731	arg1	coatings					733:740	The produced layered coatings	712:740	The produced layered coatings	712:740	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	7	32	theme	coated	1157:1162	arg1	specimens					1164:1172	The coated specimens	1153:1172	The coated specimens	1153:1172	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	3	33	used	used	616:619	arg2	parameters					600:609	the optimized EPD parameters	582:609	the optimized EPD parameters	582:609	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	6	34	theme	chitosan/BG	1028:1038	arg1	coatings					1040:1047	chitosan/BG coatings	1028:1047	chitosan/BG coatings	1028:1047	Moreover, the deposition of chitosan/BG coatings was confirmed by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR).					
30216664	4	35	theme	resistance	812:821	arg1	terms					764:768	terms	764:768	terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity	764:893	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	0	36	theme	glass	55:59	arg1	composite					75:83	bioactive glass (BG)/chitosan composite	45:83	bioactive glass (BG)/chitosan composite	45:83	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	11	37	dep	Res	1690:1692	arg1	106A					1702:1705	106A	1702:1705	106A	1702:1705	J Biomed Mater Res Part A: 106A: 3111-3122, 2018.					
30216664	11	37	dep	Res	1690:1692	arg1	A					1699:1699	Part A	1694:1699	J Biomed Mater Res Part A: 106A: 3111-3122, 2018.	1675:1723	J Biomed Mater Res Part A: 106A: 3111-3122, 2018.					
30216664	6	38	theme	Infrared	1123:1130	arg1	FTIR					1146:1149	FTIR	1146:1149	FTIR	1146:1149	Moreover, the deposition of chitosan/BG coatings was confirmed by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR).					
30216664	6	38	theme	Infrared	1123:1130	arg1	spectroscopy					1132:1143	Infrared spectroscopy	1123:1143	Infrared spectroscopy (FTIR)	1123:1150	Moreover, the deposition of chitosan/BG coatings was confirmed by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR).					
30216664	3	39	theme	glass	665:669	arg1	coatings					684:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings	632:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers	632:709	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	8	40	from	presence	1345:1352	arg1	layer					1376:1380	the top layer	1368:1380	the top layer	1368:1380	The presence of lawsone in the top layer provided antibacterial effects against Staphylococcus carnosus.					
30216664	7	41	theme	316-L	1253:1257	arg1	steel					1269:1273	bare 316-L stainless steel	1248:1273	bare 316-L stainless steel	1248:1273	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	3	42	theme	bioresorbable	632:644	arg1	coatings					684:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings	632:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers	632:709	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	1	43	theme	/lawsone	210:217	arg1	coatings					219:226	chitosan/bioactive glass (BG)/lawsone coatings	181:226	chitosan/bioactive glass (BG)/lawsone coatings	181:226	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	3	44	theme	/lawsone	675:682	arg1	coatings					684:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings	632:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers	632:709	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	10	45	dep	©	1644:1644	arg1	Inc.					1670:1673	Inc.	1670:1673	Inc.	1670:1673	© 2018 Wiley Periodicals, Inc.					
30216664	3	46	theme	BG	672:673	arg1	coatings					684:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings	632:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers	632:709	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	1	47	theme	antibacterial	400:412	arg1	coatings					414:421	bioactive and antibacterial coatings	386:421	bioactive and antibacterial coatings	386:421	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	4	48	theme	release	851:857	arg1	kinetics					859:866	drug release kinetics	846:866	drug release kinetics	846:866	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	11	49	theme	Part	1694:1697	arg1	A					1699:1699	Part A	1694:1699	J Biomed Mater Res Part A: 106A: 3111-3122, 2018.	1675:1723	J Biomed Mater Res Part A: 106A: 3111-3122, 2018.					
30216664	3	50	from	coatings	684:691	arg1	layers					704:709	PEEK/BG layers	696:709	PEEK/BG layers	696:709	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	1	51	from	deposition	262:271	arg1	layers					313:318	polyetheretherketone (PEEK)/BG layers	282:318	polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel)	282:373	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	5	52	from	coatings	990:997	arg1	release					962:968	the release	958:968	the release of lawsone from the coatings	958:997	Ultraviolet/Visible (UV/VIS) spectroscopic analyses confirmed the release of lawsone from the coatings.					
30216664	0	53	theme	/BG	115:117	arg1	layers					119:124	polyetheretherketone (PEEK)/BG layers	88:124	polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating	88:163	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	4	54	theme	corrosion	802:810	arg1	resistance					812:821	corrosion resistance	802:821	corrosion resistance	802:821	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	2	55	theme	suspension	507:516	arg1	stability					518:526	suspension stability	507:526	suspension stability	507:526	First, the EPD of chitosan/BG/lawsone was optimized on stainless steel in terms of suspension stability, homogeneity and thickness of coatings.					
30216664	1	56	theme	316-L	352:356	arg1	steel					368:372	316-L stainless steel	352:372	316-L stainless steel	352:372	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	9	57	theme	apatite-like	1486:1497	arg1	crystals					1499:1506	apatite-like crystals	1486:1506	apatite-like crystals upon immersion in simulated body fluid	1486:1545	Moreover, the developed coatings formed apatite-like crystals upon immersion in simulated body fluid, indicating the possibility of achieving close interaction between the coating surface and bone.					
30216664	7	58	from	resistance	1201:1210	arg1	comparison					1226:1235	comparison	1226:1235	comparison	1226:1235	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	3	59	theme	optimized	586:594	arg1	parameters					600:609	the optimized EPD parameters	582:609	the optimized EPD parameters	582:609	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	4	60	theme	activity	886:893	arg1	terms					764:768	terms	764:768	terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity	764:893	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	1	61	theme	bioactive	386:394	arg1	coatings					414:421	bioactive and antibacterial coatings	386:421	bioactive and antibacterial coatings	386:421	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	9	62	theme	body	1536:1539	arg1	fluid					1541:1545	simulated body fluid	1526:1545	simulated body fluid	1526:1545	Moreover, the developed coatings formed apatite-like crystals upon immersion in simulated body fluid, indicating the possibility of achieving close interaction between the coating surface and bone.					
30216664	8	63	attach	presence	1345:1352	arg1	layer					1376:1380	the top layer	1368:1380	the top layer	1368:1380	The presence of lawsone in the top layer provided antibacterial effects against Staphylococcus carnosus.					
30216664	8	63	attach	presence	1345:1352	arg2	lawsone					1357:1363	lawsone	1357:1363	lawsone	1357:1363	The presence of lawsone in the top layer provided antibacterial effects against Staphylococcus carnosus.					
30216664	1	64	theme	/BG	309:311	arg1	layers					313:318	polyetheretherketone (PEEK)/BG layers	282:318	polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel)	282:373	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	0	65	theme	polyetheretherketone	88:107	arg1	layers					119:124	polyetheretherketone (PEEK)/BG layers	88:124	polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating	88:163	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	11	66	dep	106A	1702:1705	arg1	3111-3122					1708:1716	3111-3122	1708:1716	3111-3122	1708:1716	J Biomed Mater Res Part A: 106A: 3111-3122, 2018.					
30216664	0	67	theme	PEEK	110:113	arg1	layers					119:124	polyetheretherketone (PEEK)/BG layers	88:124	polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating	88:163	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	5	68	theme	lawsone	973:979	arg1	release					962:968	the release	958:968	the release of lawsone from the coatings	958:997	Ultraviolet/Visible (UV/VIS) spectroscopic analyses confirmed the release of lawsone from the coatings.					
30216664	7	69	theme	protein	1321:1327	arg1	attachment					1329:1338	initial protein attachment	1313:1338	initial protein attachment	1313:1338	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	7	70	theme	higher	1184:1189	arg1	resistance					1201:1210	higher corrosion resistance	1184:1210	higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel	1184:1273	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	7	70	theme	higher	1184:1189	arg1	times					1216:1220	10 times	1213:1220	10 times	1213:1220	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	5	71	theme	spectroscopic	925:937	arg1	analyses					939:946	Ultraviolet/Visible (UV/VIS) spectroscopic analyses	896:946	Ultraviolet/Visible (UV/VIS) spectroscopic analyses	896:946	Ultraviolet/Visible (UV/VIS) spectroscopic analyses confirmed the release of lawsone from the coatings.					
30216664	6	72	theme	Scanning	1066:1073	arg1	SEM					1096:1098	SEM	1096:1098	SEM	1096:1098	Moreover, the deposition of chitosan/BG coatings was confirmed by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR).					
30216664	6	72	theme	Scanning	1066:1073	arg1	Microscopy					1084:1093	Scanning Electron Microscopy	1066:1093	Scanning Electron Microscopy (SEM)	1066:1099	Moreover, the deposition of chitosan/BG coatings was confirmed by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR).					
30216664	8	73	theme	lawsone	1357:1363	arg1	presence					1345:1352	The presence	1341:1352	The presence of lawsone in the top layer	1341:1380	The presence of lawsone in the top layer provided antibacterial effects against Staphylococcus carnosus.					
30216664	4	74	dep	in	824:825	arg1	vitro					827:831	vitro	827:831	vitro	827:831	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	7	75	theme	convenient	1286:1295	arg1	wettability					1297:1307	convenient wettability	1286:1307	convenient wettability for initial protein attachment	1286:1338	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	4	76	theme	produced	716:723	arg1	coatings					733:740	The produced layered coatings	712:740	The produced layered coatings	712:740	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	0	77	theme	bioactive	45:53	arg1	composite					75:83	bioactive glass (BG)/chitosan composite	45:83	bioactive glass (BG)/chitosan composite	45:83	Electrophoretic deposition of lawsone loaded bioactive glass (BG)/chitosan composite on polyetheretherketone (PEEK)/BG layers as antibacterial and bioactive coating.					
30216664	1	78	theme	glass	200:204	arg1	coatings					219:226	chitosan/bioactive glass (BG)/lawsone coatings	181:226	chitosan/bioactive glass (BG)/lawsone coatings	181:226	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	9	79	theme	coating	1618:1624	arg1	surface					1626:1632	the coating surface	1614:1632	the coating surface	1614:1632	Moreover, the developed coatings formed apatite-like crystals upon immersion in simulated body fluid, indicating the possibility of achieving close interaction between the coating surface and bone.					
30216664	6	80	theme	coatings	1040:1047	arg1	deposition					1014:1023	the deposition	1010:1023	the deposition of chitosan/BG coatings	1010:1047	Moreover, the deposition of chitosan/BG coatings was confirmed by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR).					
30216664	4	81	theme	microstructure	786:799	arg1	terms					764:768	terms	764:768	terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity	764:893	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
30216664	1	82	theme	polyetheretherketone	282:301	arg1	layers					313:318	polyetheretherketone (PEEK)/BG layers	282:318	polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel)	282:373	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	9	83	from	crystals	1499:1506	arg1	fluid					1541:1545	simulated body fluid	1526:1545	simulated body fluid	1526:1545	Moreover, the developed coatings formed apatite-like crystals upon immersion in simulated body fluid, indicating the possibility of achieving close interaction between the coating surface and bone.					
30216664	7	84	theme	stainless	1259:1267	arg1	steel					1269:1273	bare 316-L stainless steel	1248:1273	bare 316-L stainless steel	1248:1273	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	3	85	theme	chitosan/bioactive	646:663	arg1	coatings					684:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings	632:691	bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers	632:709	Subsequently, the optimized EPD parameters were used to produce bioresorbable chitosan/bioactive glass (BG)/lawsone coatings on PEEK/BG layers.					
30216664	8	86	theme	antibacterial	1391:1403	arg1	effects					1405:1411	antibacterial effects	1391:1411	antibacterial effects	1391:1411	The presence of lawsone in the top layer provided antibacterial effects against Staphylococcus carnosus.					
30216664	1	87	theme	PEEK	304:307	arg1	layers					313:318	polyetheretherketone (PEEK)/BG layers	282:318	polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel)	282:373	In this study, chitosan/bioactive glass (BG)/lawsone coatings were deposited by electrophoretic deposition (EPD) on polyetheretherketone (PEEK)/BG layers (previously deposited by EPD on 316-L stainless steel) to produce bioactive and antibacterial coatings.					
30216664	7	88	theme	bare	1248:1251	arg1	steel					1269:1273	bare 316-L stainless steel	1248:1273	bare 316-L stainless steel	1248:1273	The coated specimens presented higher corrosion resistance (10 times) in comparison to that of bare 316-L stainless steel and showed convenient wettability for initial protein attachment.					
30216664	6	89	dep	Fourier	1105:1111	arg1	Transform					1113:1121	Transform	1113:1121	Transform Infrared spectroscopy (FTIR)	1113:1150	Moreover, the deposition of chitosan/BG coatings was confirmed by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR).					
30216664	4	90	theme	composition	773:783	arg1	terms					764:768	terms	764:768	terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity	764:893	The produced layered coatings were characterized in terms of composition, microstructure, corrosion resistance, in vitro bioactivity, drug release kinetics and antibacterial activity.					
31702834	6	0	theme	starch	1316:1321	arg1	enzymes					1333:1339	starch degrading enzymes	1316:1339	starch degrading enzymes isoamylase III, alpha-, and beta-amylase	1316:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	0	theme	starch	1316:1321	arg1	isoamylase					1341:1350	isoamylase III	1341:1354	isoamylase III	1341:1354	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	0	theme	starch	1316:1321	arg1	alpha-					1357:1362	alpha-	1357:1362	alpha-	1357:1362	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	0	theme	starch	1316:1321	arg1	beta-amylase					1369:1380	beta-amylase	1369:1380	beta-amylase	1369:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	1	from	metabolism	1080:1089	arg1	grains					1105:1110	developing grains	1094:1110	developing grains	1094:1110	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	1	2	theme	genetic	243:249	arg1	gains					251:255	the genetic gains	239:255	the genetic gains	239:255	Unlike sporadic daytime heat spikes, a consistent increase in night-time temperatures can potentially derail the genetic gains being achieved.					
31702834	7	3	theme	poor	1507:1510	arg1	accumulation					1519:1530	poor starch accumulation	1507:1530	poor starch accumulation in grains	1507:1540	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	6	4	theme	adenosine	1199:1207	arg1	subunit					1253:1259	adenosine diphosphate glucose pyrophosphorylase small subunit	1199:1259	adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1199:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	4	5	theme	laser	597:601	arg1	micrographs					612:622	Confocal laser scanning micrographs	588:622	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue	588:666	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	7	6	theme	key	1455:1457	arg1	enzymes					1459:1465	the key enzymes	1451:1465	the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains	1451:1540	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	4	7	theme	germ	656:659	arg1	tissue					661:666	germ tissue	656:666	germ tissue	656:666	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	3	8	theme	consistent	533:542	arg1	decline					544:550	a consistent decline	531:550	a consistent decline in yields and quality	531:572	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	6	9	theme	-induced	1156:1163	arg1	reduction					1165:1173	a high night-time temperature (HNT)-induced reduction	1121:1173	a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1121:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	2	10	theme	wheat	284:288	arg1	genotypes					290:298	Ten winter wheat genotypes	273:298	Ten winter wheat genotypes	273:298	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	1	11	dep	derail	232:237	arg1	achieved					263:270	achieved	263:270	being achieved	257:270	Unlike sporadic daytime heat spikes, a consistent increase in night-time temperatures can potentially derail the genetic gains being achieved.					
31702834	6	12	theme	starch	1273:1278	arg1	synthesis					1280:1288	starch synthesis	1273:1288	starch synthesis	1273:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	0	13	theme	winter	116:121	arg1	wheat					123:127	winter wheat	116:127	winter wheat	116:127	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	1	14	theme	night-time	192:201	arg1	temperatures					203:214	night-time temperatures	192:214	night-time temperatures	192:214	Unlike sporadic daytime heat spikes, a consistent increase in night-time temperatures can potentially derail the genetic gains being achieved.					
31702834	7	15	theme	threshold	1407:1415	arg1	changes					1438:1444	The identified critical threshold, grain compositional changes	1383:1444	changes	1438:1444	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	4	16	theme	lipid	716:720	arg1	accumulation					691:702	differential accumulation	678:702	differential accumulation of protein, lipid, and starch	678:732	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	7	17	from	changes	1438:1444	arg1	metabolism					1483:1492	grain starch metabolism	1470:1492	grain starch metabolism that lead to poor starch accumulation in grains	1470:1540	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	6	18	theme	small	1247:1251	arg1	subunit					1253:1259	adenosine diphosphate glucose pyrophosphorylase small subunit	1199:1259	adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1199:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	7	19	theme	identified	1387:1396	arg1	changes					1438:1444	The identified critical threshold, grain compositional changes	1383:1444	changes	1438:1444	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	0	20	from	protein	81:87	arg1	wheat					123:127	winter wheat	116:127	winter wheat	116:127	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	6	21	theme	glucose	1221:1227	arg1	subunit					1253:1259	adenosine diphosphate glucose pyrophosphorylase small subunit	1199:1259	adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1199:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	4	22	theme	protein	707:713	arg1	accumulation					691:702	differential accumulation	678:702	differential accumulation of protein, lipid, and starch	678:732	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	1	23	theme	daytime	146:152	arg1	spikes					159:164	sporadic daytime heat spikes	137:164	sporadic daytime heat spikes	137:164	Unlike sporadic daytime heat spikes, a consistent increase in night-time temperatures can potentially derail the genetic gains being achieved.					
31702834	6	24	theme	genes	1008:1012	arg1	analysis					996:1003	Expression analysis	985:1003	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains	985:1110	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	25	theme	pyrophosphorylase	1229:1245	arg1	subunit					1253:1259	adenosine diphosphate glucose pyrophosphorylase small subunit	1199:1259	adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1199:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	5	26	theme	tolerant	963:970	arg1	Monument					975:982	the tolerant SY Monument	959:982	the tolerant SY Monument	959:982	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	5	27	from	increase	823:830	arg1	lipid					847:851	lipid	847:851	lipid	847:851	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	5	27	from	increase	823:830	arg1	endosperm					864:872	central endosperm	856:872	central endosperm	856:872	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	5	27	from	increase	823:830	arg1	protein					835:841	protein	835:841	protein	835:841	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	5	27	from	increase	823:830	arg1	starch					809:814	starch	809:814	starch	809:814	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	2	28	theme	grain-filling	385:397	arg1	stages					399:404	flowering and grain-filling stages	371:404	flowering and grain-filling stages in controlled environment chambers	371:439	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	0	29	from	changes	31:37	arg1	metabolism					55:64	grain starch metabolism	42:64	grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat	42:127	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	2	30	theme	flowering	371:379	arg1	stages					399:404	flowering and grain-filling stages	371:404	flowering and grain-filling stages in controlled environment chambers	371:439	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	0	31	theme	High	0:3	arg1	temperature					11:21	High night temperature	0:21	High night temperature	0:21	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	6	32	theme	grain-starch	1067:1078	arg1	metabolism					1080:1089	grain-starch metabolism	1067:1089	grain-starch metabolism in developing grains	1067:1110	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	4	33	theme	night-time	750:759	arg1	temperatures					761:772	night-time temperatures	750:772	night-time temperatures	750:772	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	2	34	theme	environment	420:430	arg1	chambers					432:439	controlled environment chambers	409:439	controlled environment chambers	409:439	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	7	35	from	tolerance	1597:1605	arg1	wheat					1610:1614	wheat	1610:1614	wheat	1610:1614	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	0	36	dep	metabolism	55:64	arg1	alters					66:71	alters	66:71	alters starch, protein, and lipid accumulation in winter wheat	66:127	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	6	37	from	reduction	1165:1173	arg1	levels					1189:1194	transcript levels	1178:1194	transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1178:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	37	from	reduction	1165:1173	arg1	enzymes					1333:1339	starch degrading enzymes	1316:1339	starch degrading enzymes isoamylase III, alpha-, and beta-amylase	1316:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	37	from	reduction	1165:1173	arg1	isoamylase					1341:1350	isoamylase III	1341:1354	isoamylase III	1341:1354	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	37	from	reduction	1165:1173	arg1	alpha-					1357:1362	alpha-	1357:1362	alpha-	1357:1362	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	37	from	reduction	1165:1173	arg1	beta-amylase					1369:1380	beta-amylase	1369:1380	beta-amylase	1369:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	5	38	theme	central	856:862	arg1	endosperm					864:872	central endosperm	856:872	central endosperm	856:872	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	7	39	theme	foundational	1556:1567	arg1	knowledge					1569:1577	the foundational knowledge	1552:1577	the foundational knowledge for enhancing HNT tolerance in wheat	1552:1614	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	2	40	theme	night-time	330:339	arg1	15-27°C					355:361	15-27°C	355:361	15-27°C	355:361	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	2	40	theme	night-time	330:339	arg1	temperatures					341:352	six different night-time temperatures	316:352	six different night-time temperatures (15-27°C)	316:362	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	0	41	theme	grain	42:46	arg1	metabolism					55:64	grain starch metabolism	42:64	grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat	42:127	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	3	42	theme	23o	486:488	arg1	C					490:490	23o C	486:490	23o C	486:490	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	4	43	theme	scanning	603:610	arg1	micrographs					612:622	Confocal laser scanning micrographs	588:622	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue	588:666	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	7	44	theme	starch	1512:1517	arg1	accumulation					1519:1530	poor starch accumulation	1507:1530	poor starch accumulation in grains	1507:1540	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	0	45	from	accumulation	100:111	arg1	wheat					123:127	winter wheat	116:127	winter wheat	116:127	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	6	46	theme	degrading	1323:1331	arg1	enzymes					1333:1339	starch degrading enzymes	1316:1339	starch degrading enzymes isoamylase III, alpha-, and beta-amylase	1316:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	46	theme	degrading	1323:1331	arg1	isoamylase					1341:1350	isoamylase III	1341:1354	isoamylase III	1341:1354	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	46	theme	degrading	1323:1331	arg1	alpha-					1357:1362	alpha-	1357:1362	alpha-	1357:1362	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	46	theme	degrading	1323:1331	arg1	beta-amylase					1369:1380	beta-amylase	1369:1380	beta-amylase	1369:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	3	47	theme	critical	499:506	arg1	threshold					508:516	the critical threshold	495:516	the critical threshold beyond which a consistent decline in yields and quality was observed	495:585	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	3	47	theme	critical	499:506	arg1	temperature					471:481	the night-time temperature	456:481	the night-time temperature of 23o C	456:490	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	2	48	theme	winter	277:282	arg1	genotypes					290:298	Ten winter wheat genotypes	273:298	Ten winter wheat genotypes	273:298	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	7	49	theme	grain	1470:1474	arg1	metabolism					1483:1492	grain starch metabolism	1470:1492	grain starch metabolism that lead to poor starch accumulation in grains	1470:1540	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	4	50	theme	Confocal	588:595	arg1	micrographs					612:622	Confocal laser scanning micrographs	588:622	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue	588:666	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	6	51	theme	≥2-fold	1296:1302	arg1	increase					1304:1311	a ≥2-fold increase	1294:1311	a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase	1294:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	1	52	theme	heat	154:157	arg1	spikes					159:164	sporadic daytime heat spikes	137:164	sporadic daytime heat spikes	137:164	Unlike sporadic daytime heat spikes, a consistent increase in night-time temperatures can potentially derail the genetic gains being achieved.					
31702834	7	53	theme	starch	1476:1481	arg1	metabolism					1483:1492	grain starch metabolism	1470:1492	grain starch metabolism that lead to poor starch accumulation in grains	1470:1540	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	6	54	theme	transcript	1178:1187	arg1	levels					1189:1194	transcript levels	1178:1194	transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1178:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	55	dep	enzymes	1333:1339	arg1	enzymes					1333:1339	starch degrading enzymes	1316:1339	starch degrading enzymes isoamylase III, alpha-, and beta-amylase	1316:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	55	dep	enzymes	1333:1339	arg1	isoamylase					1341:1350	isoamylase III	1341:1354	isoamylase III	1341:1354	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	55	dep	enzymes	1333:1339	arg1	alpha-					1357:1362	alpha-	1357:1362	alpha-	1357:1362	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	55	dep	enzymes	1333:1339	arg1	beta-amylase					1369:1380	beta-amylase	1369:1380	beta-amylase	1369:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	56	theme	Expression	985:994	arg1	analysis					996:1003	Expression analysis	985:1003	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains	985:1110	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	4	57	theme	tissue	661:666	arg1	micrographs					612:622	Confocal laser scanning micrographs	588:622	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue	588:666	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	1	58	theme	consistent	169:178	arg1	increase					180:187	a consistent increase	167:187	a consistent increase in night-time temperatures	167:214	Unlike sporadic daytime heat spikes, a consistent increase in night-time temperatures can potentially derail the genetic gains being achieved.					
31702834	5	59	dep	recorded	786:793	arg1	whereas					915:921	whereas	915:921	whereas	915:921	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	4	60	theme	endosperm	635:643	arg1	micrographs					612:622	Confocal laser scanning micrographs	588:622	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue	588:666	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	7	61	theme	compositional	1424:1436	arg1	changes					1438:1444	The identified critical threshold, grain compositional changes	1383:1444	changes	1438:1444	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	4	62	theme	bran	646:649	arg1	micrographs					612:622	Confocal laser scanning micrographs	588:622	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue	588:666	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	7	63	from	accumulation	1519:1530	arg1	grains					1535:1540	grains	1535:1540	grains	1535:1540	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	6	64	theme	subunit	1253:1259	arg1	levels					1189:1194	transcript levels	1178:1194	transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1178:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	1	65	from	increase	180:187	arg1	temperatures					203:214	night-time temperatures	192:214	night-time temperatures	192:214	Unlike sporadic daytime heat spikes, a consistent increase in night-time temperatures can potentially derail the genetic gains being achieved.					
31702834	6	66	theme	including	1035:1043	arg1	enzymes					1026:1032	21 enzymes	1023:1032	21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains	1023:1110	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	66	theme	including	1035:1043	arg1	isoforms					1045:1052	including isoforms	1035:1052	including isoforms	1035:1052	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	7	67	theme	critical	1398:1405	arg1	changes					1438:1444	The identified critical threshold, grain compositional changes	1383:1444	changes	1438:1444	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	5	68	theme	night-time	890:899	arg1	temperatures					901:912	night-time temperatures	890:912	night-time temperatures	890:912	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	6	69	theme	diphosphate	1209:1219	arg1	subunit					1253:1259	adenosine diphosphate glucose pyrophosphorylase small subunit	1199:1259	adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1199:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	4	70	theme	differential	678:689	arg1	accumulation					691:702	differential accumulation	678:702	differential accumulation of protein, lipid, and starch	678:732	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	1	71	theme	sporadic	137:144	arg1	spikes					159:164	sporadic daytime heat spikes	137:164	sporadic daytime heat spikes	137:164	Unlike sporadic daytime heat spikes, a consistent increase in night-time temperatures can potentially derail the genetic gains being achieved.					
31702834	7	72	from	enzymes	1459:1465	arg1	metabolism					1483:1492	grain starch metabolism	1470:1492	grain starch metabolism that lead to poor starch accumulation in grains	1470:1540	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	3	73	from	decline	544:550	arg1	quality					566:572	quality	566:572	quality	566:572	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	3	73	from	decline	544:550	arg1	yields					555:560	yields	555:560	yields	555:560	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	5	74	from	decrease	797:804	arg1	lipid					847:851	lipid	847:851	lipid	847:851	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	5	74	from	decrease	797:804	arg1	endosperm					864:872	central endosperm	856:872	central endosperm	856:872	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	5	74	from	decrease	797:804	arg1	protein					835:841	protein	835:841	protein	835:841	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	5	74	from	decrease	797:804	arg1	starch					809:814	starch	809:814	starch	809:814	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	5	75	theme	SY	972:973	arg1	Monument					975:982	the tolerant SY Monument	959:982	the tolerant SY Monument	959:982	KS07077M-1 recorded a decrease in starch and an increase in protein and lipid in central endosperm with increasing night-time temperatures, whereas the same was significantly lower in the tolerant SY Monument.					
31702834	7	76	theme	HNT	1593:1595	arg1	tolerance					1597:1605	HNT tolerance	1593:1605	HNT tolerance in wheat	1593:1614	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	0	77	theme	lipid	94:98	arg1	accumulation					100:111	lipid accumulation	94:111	lipid accumulation	94:111	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	6	78	theme	developing	1094:1103	arg1	grains					1105:1110	developing grains	1094:1110	developing grains	1094:1110	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	0	79	theme	night	5:9	arg1	temperature					11:21	High night temperature	0:21	High night temperature	0:21	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	4	80	theme	starch	727:732	arg1	accumulation					691:702	differential accumulation	678:702	differential accumulation of protein, lipid, and starch	678:732	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
31702834	0	81	from	starch	73:78	arg1	wheat					123:127	winter wheat	116:127	winter wheat	116:127	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	7	82	theme	grain	1418:1422	arg1	changes					1438:1444	The identified critical threshold, grain compositional changes	1383:1444	changes	1438:1444	The identified critical threshold, grain compositional changes, and the key enzymes in grain starch metabolism that lead to poor starch accumulation in grains establish the foundational knowledge for enhancing HNT tolerance in wheat.					
31702834	2	83	theme	controlled	409:418	arg1	chambers					432:439	controlled environment chambers	409:439	controlled environment chambers	409:439	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	3	84	theme	night-time	460:469	arg1	threshold					508:516	the critical threshold	495:516	the critical threshold beyond which a consistent decline in yields and quality was observed	495:585	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	3	84	theme	night-time	460:469	arg1	temperature					471:481	the night-time temperature	456:481	the night-time temperature of 23o C	456:490	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	0	85	theme	starch	48:53	arg1	metabolism					55:64	grain starch metabolism	42:64	grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat	42:127	High night temperature induced changes in grain starch metabolism alters starch, protein, and lipid accumulation in winter wheat.					
31702834	2	86	theme	different	320:328	arg1	15-27°C					355:361	15-27°C	355:361	15-27°C	355:361	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	2	86	theme	different	320:328	arg1	temperatures					341:352	six different night-time temperatures	316:352	six different night-time temperatures (15-27°C)	316:362	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	3	87	theme	C	490:490	arg1	threshold					508:516	the critical threshold	495:516	the critical threshold beyond which a consistent decline in yields and quality was observed	495:585	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	3	87	theme	C	490:490	arg1	temperature					471:481	the night-time temperature	456:481	the night-time temperature of 23o C	456:490	We identified the night-time temperature of 23o C as the critical threshold beyond which a consistent decline in yields and quality was observed.					
31702834	6	88	from	increase	1304:1311	arg1	levels					1189:1194	transcript levels	1178:1194	transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis	1178:1288	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	88	from	increase	1304:1311	arg1	enzymes					1333:1339	starch degrading enzymes	1316:1339	starch degrading enzymes isoamylase III, alpha-, and beta-amylase	1316:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	88	from	increase	1304:1311	arg1	isoamylase					1341:1350	isoamylase III	1341:1354	isoamylase III	1341:1354	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	88	from	increase	1304:1311	arg1	alpha-					1357:1362	alpha-	1357:1362	alpha-	1357:1362	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	6	88	from	increase	1304:1311	arg1	beta-amylase					1369:1380	beta-amylase	1369:1380	beta-amylase	1369:1380	Expression analysis of genes encoding 21 enzymes (including isoforms) involved in grain-starch metabolism in developing grains revealed a high night-time temperature (HNT)-induced reduction in transcript levels of adenosine diphosphate glucose pyrophosphorylase small subunit involved in starch synthesis and a ≥2-fold increase in starch degrading enzymes isoamylase III, alpha-, and beta-amylase.					
31702834	2	89	from	stages	399:404	arg1	chambers					432:439	controlled environment chambers	409:439	controlled environment chambers	409:439	Ten winter wheat genotypes were exposed to six different night-time temperatures (15-27°C) during flowering and grain-filling stages in controlled environment chambers.					
31702834	4	90	theme	central	627:633	arg1	endosperm					635:643	central endosperm	627:643	central endosperm	627:643	Confocal laser scanning micrographs of central endosperm, bran, and germ tissue displayed differential accumulation of protein, lipid, and starch with increasing night-time temperatures.					
30455059	3	0	from	increase	436:443	arg1	times					529:533	the corresponding T1rho relaxation times	494:533	the corresponding T1rho relaxation times	494:533	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	3	0	from	increase	436:443	arg1	concentration					461:473	proteoglycan concentration	448:473	proteoglycan concentration	448:473	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	5	1	dep	cartilage	714:722	arg1	times					741:745	T1rho relaxation times	724:745	tibial cartilage T1rho relaxation times	707:745	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	7	2	theme	MR	938:939	arg1	imaging					941:947	MR imaging	938:947	MR imaging	938:947	Subjects underwent MR imaging in the morning and afternoon on the same day and were free to go about their normal activities between scans.					
30455059	9	3	theme	relaxation	1426:1435	arg1	times					1437:1441	T1rho relaxation times	1420:1441	T1rho relaxation times	1420:1441	Additionally, baseline T1rho values varied with position within the cartilage, supporting a need for site-specific measurements of T1rho relaxation times.					
30455059	9	4	theme	baseline	1303:1310	arg1	values					1318:1323	baseline T1rho values	1303:1323	baseline T1rho values	1303:1323	Additionally, baseline T1rho values varied with position within the cartilage, supporting a need for site-specific measurements of T1rho relaxation times.					
30455059	1	5	theme	early-stage	160:170	arg1	OA					188:189	OA	188:189	OA	188:189	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	1	5	theme	early-stage	160:170	arg1	osteoarthritis					172:185	early-stage osteoarthritis	160:185	early-stage osteoarthritis (OA)	160:190	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	9	6	theme	times	1437:1441	arg1	measurements					1404:1415	site-specific measurements	1390:1415	site-specific measurements of T1rho relaxation times	1390:1441	Additionally, baseline T1rho values varied with position within the cartilage, supporting a need for site-specific measurements of T1rho relaxation times.					
30455059	5	7	theme	human	758:762	arg1	subjects					764:771	healthy human subjects	750:771	healthy human subjects	750:771	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	9	8	theme	T1rho	1312:1316	arg1	values					1318:1323	baseline T1rho values	1303:1323	baseline T1rho values	1303:1323	Additionally, baseline T1rho values varied with position within the cartilage, supporting a need for site-specific measurements of T1rho relaxation times.					
30455059	10	9	theme	clinical	1538:1545	arg1	significance					1547:1558	clinical significance	1538:1558	clinical significance pertaining to cartilage homeostasis	1538:1594	Understanding how loading alters the proteoglycan concentration in healthy cartilage may hold clinical significance pertaining to cartilage homeostasis and potentially help to elucidate a mechanism for OA development.					
30455059	11	10	theme	future	1695:1700	arg1	studies					1702:1708	future studies	1695:1708	future studies using T1rho relaxation times as an indicator of cartilage health	1695:1773	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	7	11	from	morning	956:962	arg1	day					990:992	the same day	981:992	the same day	981:992	Subjects underwent MR imaging in the morning and afternoon on the same day and were free to go about their normal activities between scans.					
30455059	11	12	theme	image	1819:1823	arg1	acquisition					1825:1835	image acquisition	1819:1835	image acquisition	1819:1835	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	7	13	theme	same	985:988	arg1	day					990:992	the same day	981:992	the same day	981:992	Subjects underwent MR imaging in the morning and afternoon on the same day and were free to go about their normal activities between scans.					
30455059	4	14	theme	daily	625:629	arg1	activity					631:638	daily activity	625:638	daily activity	625:638	To date, there is limited information on changes in cartilage composition resulting from daily activity.					
30455059	5	15	theme	following	773:781	arg1	activities					783:792	following activities	773:792	following activities of daily living	773:808	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	3	16	from	exudation	414:422	arg1	times					529:533	the corresponding T1rho relaxation times	494:533	the corresponding T1rho relaxation times	494:533	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	3	16	from	exudation	414:422	arg1	concentration					461:473	proteoglycan concentration	448:473	proteoglycan concentration	448:473	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	10	17	theme	healthy	1511:1517	arg1	cartilage					1519:1527	healthy cartilage	1511:1527	healthy cartilage	1511:1527	Understanding how loading alters the proteoglycan concentration in healthy cartilage may hold clinical significance pertaining to cartilage homeostasis and potentially help to elucidate a mechanism for OA development.					
30455059	6	18	theme	water	836:840	arg1	exudation					842:850	water exudation	836:850	water exudation throughout the day	836:869	It was hypothesized that water exudation throughout the day would lead to decreased T1rho relaxation times.					
30455059	11	19	theme	appropriate	1851:1861	arg1	interpretation					1863:1876	the appropriate interpretation	1847:1876	the appropriate interpretation of the data	1847:1888	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	11	20	theme	T1rho	1716:1720	arg1	times					1733:1737	T1rho relaxation times	1716:1737	T1rho relaxation times	1716:1737	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	11	20	theme	T1rho	1716:1720	arg1	indicator					1745:1753	an indicator	1742:1753	an indicator of cartilage health	1742:1773	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	6	21	theme	decreased	885:893	arg1	times					912:916	decreased T1rho relaxation times	885:916	decreased T1rho relaxation times	885:916	It was hypothesized that water exudation throughout the day would lead to decreased T1rho relaxation times.					
30455059	1	22	theme	Quantitative	78:89	arg1	MRI					125:127	MRI	125:127	MRI	125:127	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	1	22	theme	Quantitative	78:89	arg1	imaging					116:122	Quantitative T1rho magnetic resonance imaging	78:122	Quantitative T1rho magnetic resonance imaging (MRI)	78:128	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	8	23	theme	day	1197:1199	arg1	course					1183:1188	the course	1179:1188	the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration	1179:1286	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	11	24	theme	relaxation	1722:1731	arg1	times					1733:1737	T1rho relaxation times	1716:1737	T1rho relaxation times	1716:1737	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	11	24	theme	relaxation	1722:1731	arg1	indicator					1745:1753	an indicator	1742:1753	an indicator of cartilage health	1742:1773	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	1	25	theme	T1rho	91:95	arg1	resonance					106:114	T1rho magnetic resonance	91:114	Quantitative T1rho magnetic resonance imaging (MRI)	78:128	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	8	26	theme	cartilage	1109:1117	arg1	times					1136:1140	tibial cartilage T1rho relaxation times	1102:1140	tibial cartilage T1rho relaxation times	1102:1140	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	8	27	theme	increase	1249:1256	arg1	day					1197:1199	the day	1193:1199	the day	1193:1199	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	8	27	theme	increase	1249:1256	arg1	indicative					1224:1233	indicative	1224:1233	indicative	1224:1233	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	8	28	dep	decreased	1156:1164	arg1	%					1171:1171	7%	1170:1171	7%	1170:1171	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	5	29	theme	study	674:678	arg1	objective					656:664	the objective	652:664	the objective of this study	652:678	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	8	30	from	increase	1249:1256	arg1	concentration					1274:1286	proteoglycan concentration	1261:1286	proteoglycan concentration	1261:1286	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	1	31	theme	magnetic	97:104	arg1	resonance					106:114	T1rho magnetic resonance	91:114	Quantitative T1rho magnetic resonance imaging (MRI)	78:128	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	8	32	theme	relative	1240:1247	arg1	increase					1249:1256	a relative increase	1238:1256	a relative increase in proteoglycan concentration	1238:1286	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	3	33	theme	water	408:412	arg1	exudation					414:422	water exudation	408:422	water exudation	408:422	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	5	34	theme	relaxation	730:739	arg1	times					741:745	T1rho relaxation times	724:745	tibial cartilage T1rho relaxation times	707:745	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	7	35	dep	morning	956:962	arg1	the					952:954	the	952:954	the	952:954	Subjects underwent MR imaging in the morning and afternoon on the same day and were free to go about their normal activities between scans.					
30455059	1	36	theme	resonance	106:114	arg1	MRI					125:127	MRI	125:127	MRI	125:127	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	1	36	theme	resonance	106:114	arg1	imaging					116:122	Quantitative T1rho magnetic resonance imaging	78:122	Quantitative T1rho magnetic resonance imaging (MRI)	78:128	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	10	37	theme	proteoglycan	1481:1492	arg1	concentration					1494:1506	the proteoglycan concentration	1477:1506	the proteoglycan concentration in healthy cartilage	1477:1527	Understanding how loading alters the proteoglycan concentration in healthy cartilage may hold clinical significance pertaining to cartilage homeostasis and potentially help to elucidate a mechanism for OA development.					
30455059	8	38	theme	proteoglycan	1261:1272	arg1	concentration					1274:1286	proteoglycan concentration	1261:1286	proteoglycan concentration	1261:1286	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	0	39	theme	daily	14:18	arg1	Activities					0:9	Activities	0:9	Activities of daily living	0:25	Activities of daily living influence tibial cartilage T1rho relaxation times.					
30455059	10	40	theme	cartilage	1574:1582	arg1	homeostasis					1584:1594	cartilage homeostasis	1574:1594	cartilage homeostasis	1574:1594	Understanding how loading alters the proteoglycan concentration in healthy cartilage may hold clinical significance pertaining to cartilage homeostasis and potentially help to elucidate a mechanism for OA development.					
30455059	8	41	theme	tibial	1102:1107	arg1	times					1136:1140	tibial cartilage T1rho relaxation times	1102:1140	tibial cartilage T1rho relaxation times	1102:1140	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	2	42	theme	proteoglycan	325:336	arg1	concentration					338:350	proteoglycan concentration	325:350	proteoglycan concentration	325:350	T1rho relaxation times are negatively correlated with proteoglycan concentration.					
30455059	3	43	theme	corresponding	498:510	arg1	times					529:533	the corresponding T1rho relaxation times	494:533	the corresponding T1rho relaxation times	494:533	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	0	44	theme	tibial	37:42	arg1	times					71:75	tibial cartilage T1rho relaxation times	37:75	tibial cartilage T1rho relaxation times	37:75	Activities of daily living influence tibial cartilage T1rho relaxation times.					
30455059	4	45	theme	cartilage	588:596	arg1	composition					598:608	cartilage composition	588:608	cartilage composition resulting from daily activity	588:638	To date, there is limited information on changes in cartilage composition resulting from daily activity.					
30455059	8	46	theme	relaxation	1125:1134	arg1	times					1136:1140	tibial cartilage T1rho relaxation times	1102:1140	tibial cartilage T1rho relaxation times	1102:1140	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	6	47	theme	relaxation	901:910	arg1	times					912:916	decreased T1rho relaxation times	885:916	decreased T1rho relaxation times	885:916	It was hypothesized that water exudation throughout the day would lead to decreased T1rho relaxation times.					
30455059	5	48	theme	healthy	750:756	arg1	subjects					764:771	healthy human subjects	750:771	healthy human subjects	750:771	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	3	49	theme	proteoglycan	448:459	arg1	concentration					461:473	proteoglycan concentration	448:473	proteoglycan concentration	448:473	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	4	50	theme	limited	554:560	arg1	information					562:572	limited information	554:572	limited information on changes in cartilage composition resulting from daily activity	554:638	To date, there is limited information on changes in cartilage composition resulting from daily activity.					
30455059	4	51	from	information	562:572	arg1	changes					577:583	changes	577:583	changes in cartilage composition resulting from daily activity	577:638	To date, there is limited information on changes in cartilage composition resulting from daily activity.					
30455059	6	52	theme	T1rho	895:899	arg1	times					912:916	decreased T1rho relaxation times	885:916	decreased T1rho relaxation times	885:916	It was hypothesized that water exudation throughout the day would lead to decreased T1rho relaxation times.					
30455059	10	53	theme	OA	1646:1647	arg1	development					1649:1659	OA development	1646:1659	OA development	1646:1659	Understanding how loading alters the proteoglycan concentration in healthy cartilage may hold clinical significance pertaining to cartilage homeostasis and potentially help to elucidate a mechanism for OA development.					
30455059	3	54	from	decrease	482:489	arg1	times					529:533	the corresponding T1rho relaxation times	494:533	the corresponding T1rho relaxation times	494:533	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	3	54	from	decrease	482:489	arg1	concentration					461:473	proteoglycan concentration	448:473	proteoglycan concentration	448:473	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	1	55	theme	proteoglycan	220:231	arg1	concentration					233:245	proteoglycan concentration	220:245	proteoglycan concentration	220:245	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	3	56	theme	relative	427:434	arg1	increase					436:443	a relative increase	425:443	a relative increase in proteoglycan concentration	425:473	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	5	57	from	changes	696:702	arg1	cartilage					714:722	tibial cartilage T1rho relaxation times	707:745	tibial cartilage T1rho relaxation times	707:745	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	5	57	from	changes	696:702	arg1	subjects					764:771	healthy human subjects	750:771	healthy human subjects	750:771	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	3	58	dep	load	389:392	arg1	response					377:384	response	377:384	response	377:384	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	0	59	theme	relaxation	60:69	arg1	times					71:75	tibial cartilage T1rho relaxation times	37:75	tibial cartilage T1rho relaxation times	37:75	Activities of daily living influence tibial cartilage T1rho relaxation times.					
30455059	11	60	theme	loading	1794:1800	arg1	history					1802:1808	the loading history	1790:1808	the loading history prior to image acquisition to ensure the appropriate interpretation of the data	1790:1888	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	0	61	theme	cartilage	44:52	arg1	times					71:75	tibial cartilage T1rho relaxation times	37:75	tibial cartilage T1rho relaxation times	37:75	Activities of daily living influence tibial cartilage T1rho relaxation times.					
30455059	5	62	theme	tibial	707:712	arg1	cartilage					714:722	tibial cartilage T1rho relaxation times	707:745	tibial cartilage T1rho relaxation times	707:745	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	8	63	with	day	1197:1199	arg1	loading					1206:1212	loading	1206:1212	loading	1206:1212	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	7	64	from	afternoon	968:976	arg1	day					990:992	the same day	981:992	the same day	981:992	Subjects underwent MR imaging in the morning and afternoon on the same day and were free to go about their normal activities between scans.					
30455059	11	65	theme	data	1885:1888	arg1	interpretation					1863:1876	the appropriate interpretation	1847:1876	the appropriate interpretation of the data	1847:1888	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	11	66	theme	cartilage	1758:1766	arg1	health					1768:1773	cartilage health	1758:1773	cartilage health	1758:1773	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	5	67	theme	daily	797:801	arg1	activities					783:792	following activities	773:792	following activities of daily living	773:808	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	3	68	theme	T1rho	512:516	arg1	times					529:533	the corresponding T1rho relaxation times	494:533	the corresponding T1rho relaxation times	494:533	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	9	69	theme	site-specific	1390:1402	arg1	measurements					1404:1415	site-specific measurements	1390:1415	site-specific measurements of T1rho relaxation times	1390:1441	Additionally, baseline T1rho values varied with position within the cartilage, supporting a need for site-specific measurements of T1rho relaxation times.					
30455059	5	70	theme	T1rho	724:728	arg1	times					741:745	T1rho relaxation times	724:745	tibial cartilage T1rho relaxation times	707:745	Therefore, the objective of this study was to quantify changes in tibial cartilage T1rho relaxation times in healthy human subjects following activities of daily living.					
30455059	11	71	theme	health	1768:1773	arg1	times					1733:1737	T1rho relaxation times	1716:1737	T1rho relaxation times	1716:1737	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	11	71	theme	health	1768:1773	arg1	indicator					1745:1753	an indicator	1742:1753	an indicator of cartilage health	1742:1773	These results also indicate that future studies using T1rho relaxation times as an indicator of cartilage health should control the loading history prior to image acquisition to ensure the appropriate interpretation of the data.					
30455059	2	72	theme	relaxation	277:286	arg1	times					288:292	T1rho relaxation times	271:292	T1rho relaxation times	271:292	T1rho relaxation times are negatively correlated with proteoglycan concentration.					
30455059	3	73	theme	relaxation	518:527	arg1	times					529:533	the corresponding T1rho relaxation times	494:533	the corresponding T1rho relaxation times	494:533	Cartilage compresses in response to load, resulting in water exudation, a relative increase in proteoglycan concentration, and a decrease in the corresponding T1rho relaxation times.					
30455059	10	74	from	concentration	1494:1506	arg1	cartilage					1519:1527	healthy cartilage	1511:1527	healthy cartilage	1511:1527	Understanding how loading alters the proteoglycan concentration in healthy cartilage may hold clinical significance pertaining to cartilage homeostasis and potentially help to elucidate a mechanism for OA development.					
30455059	7	75	theme	normal	1026:1031	arg1	activities					1033:1042	their normal activities	1020:1042	their normal activities between scans	1020:1056	Subjects underwent MR imaging in the morning and afternoon on the same day and were free to go about their normal activities between scans.					
30455059	2	76	theme	T1rho	271:275	arg1	times					288:292	T1rho relaxation times	271:292	T1rho relaxation times	271:292	T1rho relaxation times are negatively correlated with proteoglycan concentration.					
30455059	1	77	theme	articular	250:258	arg1	cartilage					260:268	articular cartilage	250:268	articular cartilage	250:268	Quantitative T1rho magnetic resonance imaging (MRI) can potentially help identify early-stage osteoarthritis (OA) by non-invasively assessing proteoglycan concentration in articular cartilage.					
30455059	0	78	theme	T1rho	54:58	arg1	times					71:75	tibial cartilage T1rho relaxation times	37:75	tibial cartilage T1rho relaxation times	37:75	Activities of daily living influence tibial cartilage T1rho relaxation times.					
30455059	4	79	from	changes	577:583	arg1	composition					598:608	cartilage composition	588:608	cartilage composition resulting from daily activity	588:638	To date, there is limited information on changes in cartilage composition resulting from daily activity.					
30455059	8	80	theme	T1rho	1119:1123	arg1	times					1136:1140	tibial cartilage T1rho relaxation times	1102:1140	tibial cartilage T1rho relaxation times	1102:1140	Our findings confirmed the hypothesis that tibial cartilage T1rho relaxation times significantly decreased (by 7%) over the course of the day with loading, which is indicative of a relative increase in proteoglycan concentration.					
30455059	9	81	theme	T1rho	1420:1424	arg1	times					1437:1441	T1rho relaxation times	1420:1441	T1rho relaxation times	1420:1441	Additionally, baseline T1rho values varied with position within the cartilage, supporting a need for site-specific measurements of T1rho relaxation times.					
31332537	5	0	theme	chitinolytic	857:868	arg1	bacterium					877:885	a chitinolytic marine bacterium	855:885	a chitinolytic marine bacterium	855:885	The enzyme cocktail was extracted after fermentation in a bioreactor by Aeromonas caviae CHZ306, a chitinolytic marine bacterium with great potential for chitinase production.					
31332537	5	0	theme	chitinolytic	857:868	arg1	fermentation					798:809	fermentation	798:809	fermentation in a bioreactor by Aeromonas caviae CHZ306	798:852	The enzyme cocktail was extracted after fermentation in a bioreactor by Aeromonas caviae CHZ306, a chitinolytic marine bacterium with great potential for chitinase production.					
31332537	6	1	theme	concentration	1019:1031	arg1	terms					972:976	terms	972:976	terms of temperature, pH, enzyme and substrate concentration, and reaction time	972:1050	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	1	2	theme	important	162:170	arg1	N-acetyl-D-glucosamine					124:145	N-acetyl-D-glucosamine	124:145	N-acetyl-D-glucosamine (GlcNAc)	124:154	N-acetyl-D-glucosamine (GlcNAc) is an important amino-monosaccharide with great potential for biotechnological applications.					
31332537	1	2	theme	important	162:170	arg1	amino-monosaccharide					172:191	an important amino-monosaccharide	159:191	an important amino-monosaccharide with great potential for biotechnological applications	159:246	N-acetyl-D-glucosamine (GlcNAc) is an important amino-monosaccharide with great potential for biotechnological applications.					
31332537	5	3	theme	marine	870:875	arg1	bacterium					877:885	a chitinolytic marine bacterium	855:885	a chitinolytic marine bacterium	855:885	The enzyme cocktail was extracted after fermentation in a bioreactor by Aeromonas caviae CHZ306, a chitinolytic marine bacterium with great potential for chitinase production.					
31332537	5	3	theme	marine	870:875	arg1	fermentation					798:809	fermentation	798:809	fermentation in a bioreactor by Aeromonas caviae CHZ306	798:852	The enzyme cocktail was extracted after fermentation in a bioreactor by Aeromonas caviae CHZ306, a chitinolytic marine bacterium with great potential for chitinase production.					
31332537	6	4	theme	substrate	1009:1017	arg1	concentration					1019:1031	enzyme and substrate concentration	998:1031	enzyme and substrate concentration	998:1031	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	1	5	with	amino-monosaccharide	172:191	arg1	potential					204:212	great potential	198:212	great potential for biotechnological applications	198:246	N-acetyl-D-glucosamine (GlcNAc) is an important amino-monosaccharide with great potential for biotechnological applications.					
31332537	9	6	theme	substrate	1642:1650	arg1	structures					1652:1661	the substrate structures	1638:1661	the substrate structures	1638:1661	This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures.					
31332537	9	7	theme	enzymatic	1526:1534	arg1	approach					1536:1543	an enzymatic approach	1523:1543	an enzymatic approach	1523:1543	This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures.					
31332537	9	8	from	information	1465:1475	arg1	bioproduction					1491:1503	the GlcNAc bioproduction	1480:1503	the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures	1480:1661	This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures.					
31332537	5	9	from	fermentation	798:809	arg1	bioreactor					816:825	a bioreactor	814:825	a bioreactor by Aeromonas caviae CHZ306	814:852	The enzyme cocktail was extracted after fermentation in a bioreactor by Aeromonas caviae CHZ306, a chitinolytic marine bacterium with great potential for chitinase production.					
31332537	8	10	theme	Such	1244:1247	arg1	result					1249:1254	Such result	1244:1254	Such result	1244:1254	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	6	11	theme	enzyme	998:1003	arg1	concentration					1019:1031	enzyme and substrate concentration	998:1031	enzyme and substrate concentration	998:1031	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	5	12	theme	enzyme	762:767	arg1	cocktail					769:776	The enzyme cocktail	758:776	The enzyme cocktail	758:776	The enzyme cocktail was extracted after fermentation in a bioreactor by Aeromonas caviae CHZ306, a chitinolytic marine bacterium with great potential for chitinase production.					
31332537	6	13	theme	reaction	1038:1045	arg1	time					1047:1050	reaction time	1038:1050	reaction time	1038:1050	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	4	14	theme	enzyme	649:654	arg1	cocktail					656:663	an enzyme cocktail	646:663	an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases)	646:755	In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).					
31332537	2	15	theme	acidic	388:393	arg1	wastes					395:400	acidic wastes	388:400	acidic wastes	388:400	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	7	16	theme	yields	1183:1188	arg1	improvement					1161:1171	a substantial improvement	1147:1171	a substantial improvement of GlcNAc yields	1147:1188	The use of colloidal α-chitin as substrate showed a substantial improvement of GlcNAc yields, when compared with β-chitin and α-chitin polymorphs.					
31332537	2	17	theme	chitin	314:319	arg1	hydrolysis					300:309	the chemical hydrolysis	287:309	the chemical hydrolysis of chitin	287:319	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	9	18	theme	key	1561:1563	arg1	points					1565:1570	the key points	1557:1570	the key points for its production	1557:1589	This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures.					
31332537	6	19	theme	GlcNAc	1072:1077	arg1	yield					1079:1083	90% GlcNAc yield	1068:1083	90% GlcNAc yield within 6 h	1068:1094	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	0	20	from	α-chitin	53:60	arg1	Bioproduction					0:12	Bioproduction	0:12	Bioproduction of N-acetyl-glucosamine from colloidal α-chitin using an enzyme cocktail	0:85	Bioproduction of N-acetyl-glucosamine from colloidal α-chitin using an enzyme cocktail produced by Aeromonas caviae CHZ306.					
31332537	8	21	theme	greater	1385:1391	arg1	accessibility					1393:1405	greater accessibility	1385:1405	greater accessibility to the depolymerized chains	1385:1433	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	1	22	theme	great	198:202	arg1	potential					204:212	great potential	198:212	great potential for biotechnological applications	198:246	N-acetyl-D-glucosamine (GlcNAc) is an important amino-monosaccharide with great potential for biotechnological applications.					
31332537	4	23	from	α-chitin	631:638	arg1	bioproduction					602:614	the GlcNAc bioproduction	591:614	the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases)	591:755	In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).					
31332537	8	24	from	decrease	1293:1300	arg1	viscosity					1323:1331	viscosity	1323:1331	viscosity	1323:1331	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	8	24	from	decrease	1293:1300	arg1	α-chitin					1346:1353	natural α-chitin	1338:1353	natural α-chitin	1338:1353	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	8	24	from	decrease	1293:1300	arg1	crystallinity					1305:1317	crystallinity	1305:1317	crystallinity	1305:1317	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	2	25	theme	environmental	353:365	arg1	costs					423:427	high costs	418:427	high costs	418:427	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	25	theme	environmental	353:365	arg1	drawbacks					367:375	certain industrial and environmental drawbacks	330:375	certain industrial and environmental drawbacks	330:375	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	25	theme	environmental	353:365	arg1	wastes					395:400	acidic wastes	388:400	acidic wastes	388:400	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	25	theme	environmental	353:365	arg1	yields					407:412	low yields	403:412	low yields	403:412	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	26	theme	high	418:421	arg1	costs					423:427	high costs	418:427	high costs	418:427	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	5	27	theme	chitinase	912:920	arg1	production					922:931	chitinase production	912:931	chitinase production	912:931	The enzyme cocktail was extracted after fermentation in a bioreactor by Aeromonas caviae CHZ306, a chitinolytic marine bacterium with great potential for chitinase production.					
31332537	6	28	theme	%	1070:1070	arg1	yield					1079:1083	90% GlcNAc yield	1068:1083	90% GlcNAc yield within 6 h	1068:1094	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	7	29	theme	GlcNAc	1176:1181	arg1	yields					1183:1188	GlcNAc yields	1176:1188	GlcNAc yields	1176:1188	The use of colloidal α-chitin as substrate showed a substantial improvement of GlcNAc yields, when compared with β-chitin and α-chitin polymorphs.					
31332537	7	30	theme	β-chitin	1210:1217	arg1	polymorphs					1232:1241	β-chitin and α-chitin polymorphs	1210:1241	β-chitin and α-chitin polymorphs	1210:1241	The use of colloidal α-chitin as substrate showed a substantial improvement of GlcNAc yields, when compared with β-chitin and α-chitin polymorphs.					
31332537	8	31	theme	significant	1281:1291	arg1	decrease					1293:1300	a significant decrease	1279:1300	a significant decrease in crystallinity and viscosity from natural α-chitin	1279:1353	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	6	32	theme	90	1068:1069	arg1	%					1070:1070	%	1070:1070	%	1070:1070	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	7	33	theme	substantial	1149:1159	arg1	improvement					1161:1171	a substantial improvement	1147:1171	a substantial improvement of GlcNAc yields	1147:1188	The use of colloidal α-chitin as substrate showed a substantial improvement of GlcNAc yields, when compared with β-chitin and α-chitin polymorphs.					
31332537	2	34	theme	industrial	338:347	arg1	costs					423:427	high costs	418:427	high costs	418:427	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	34	theme	industrial	338:347	arg1	drawbacks					367:375	certain industrial and environmental drawbacks	330:375	certain industrial and environmental drawbacks	330:375	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	34	theme	industrial	338:347	arg1	wastes					395:400	acidic wastes	388:400	acidic wastes	388:400	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	34	theme	industrial	338:347	arg1	yields					407:412	low yields	403:412	low yields	403:412	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	3	35	theme	environmentally-friendly	498:521	arg1	attention					473:481	attention	473:481	attention	473:481	Therefore, enzymatic production has gained attention as a promising environmentally-friendly alternative to the chemical processes.					
31332537	3	35	theme	environmentally-friendly	498:521	arg1	alternative					523:533	a promising environmentally-friendly alternative	486:533	a promising environmentally-friendly alternative to the chemical processes	486:559	Therefore, enzymatic production has gained attention as a promising environmentally-friendly alternative to the chemical processes.					
31332537	0	36	theme	N-acetyl-glucosamine	17:36	arg1	Bioproduction					0:12	Bioproduction	0:12	Bioproduction of N-acetyl-glucosamine from colloidal α-chitin using an enzyme cocktail	0:85	Bioproduction of N-acetyl-glucosamine from colloidal α-chitin using an enzyme cocktail produced by Aeromonas caviae CHZ306.					
31332537	6	37	theme	temperature	981:991	arg1	terms					972:976	terms	972:976	terms of temperature, pH, enzyme and substrate concentration, and reaction time	972:1050	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	2	38	theme	certain	330:336	arg1	costs					423:427	high costs	418:427	high costs	418:427	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	38	theme	certain	330:336	arg1	drawbacks					367:375	certain industrial and environmental drawbacks	330:375	certain industrial and environmental drawbacks	330:375	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	38	theme	certain	330:336	arg1	wastes					395:400	acidic wastes	388:400	acidic wastes	388:400	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	2	38	theme	certain	330:336	arg1	yields					407:412	low yields	403:412	low yields	403:412	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	3	39	theme	enzymatic	441:449	arg1	production					451:460	enzymatic production	441:460	enzymatic production	441:460	Therefore, enzymatic production has gained attention as a promising environmentally-friendly alternative to the chemical processes.					
31332537	4	40	theme	colloidal	621:629	arg1	α-chitin					631:638	colloidal α-chitin	621:638	colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases)	621:755	In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).					
31332537	8	41	theme	natural	1338:1344	arg1	α-chitin					1346:1353	natural α-chitin	1338:1353	natural α-chitin	1338:1353	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	0	42	theme	colloidal	43:51	arg1	α-chitin					53:60	colloidal α-chitin	43:60	colloidal α-chitin using an enzyme cocktail	43:85	Bioproduction of N-acetyl-glucosamine from colloidal α-chitin using an enzyme cocktail produced by Aeromonas caviae CHZ306.					
31332537	8	43	from	α-chitin	1346:1353	arg1	viscosity					1323:1331	viscosity	1323:1331	viscosity	1323:1331	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	8	43	from	α-chitin	1346:1353	arg1	crystallinity					1305:1317	crystallinity	1305:1317	crystallinity	1305:1317	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	8	43	from	α-chitin	1346:1353	arg1	decrease					1293:1300	a significant decrease	1279:1300	a significant decrease in crystallinity and viscosity from natural α-chitin	1279:1353	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	3	44	theme	promising	488:496	arg1	attention					473:481	attention	473:481	attention	473:481	Therefore, enzymatic production has gained attention as a promising environmentally-friendly alternative to the chemical processes.					
31332537	3	44	theme	promising	488:496	arg1	alternative					523:533	a promising environmentally-friendly alternative	486:533	a promising environmentally-friendly alternative to the chemical processes	486:559	Therefore, enzymatic production has gained attention as a promising environmentally-friendly alternative to the chemical processes.					
31332537	5	45	theme	great	892:896	arg1	potential					898:906	great potential	892:906	great potential for chitinase production	892:931	The enzyme cocktail was extracted after fermentation in a bioreactor by Aeromonas caviae CHZ306, a chitinolytic marine bacterium with great potential for chitinase production.					
31332537	6	46	theme	time	1047:1050	arg1	terms					972:976	terms	972:976	terms of temperature, pH, enzyme and substrate concentration, and reaction time	972:1050	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	9	47	from	chitin	1510:1515	arg1	bioproduction					1491:1503	the GlcNAc bioproduction	1480:1503	the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures	1480:1661	This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures.					
31332537	6	48	theme	pH	994:995	arg1	terms					972:976	terms	972:976	terms of temperature, pH, enzyme and substrate concentration, and reaction time	972:1050	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	3	49	theme	chemical	542:549	arg1	processes					551:559	the chemical processes	538:559	the chemical processes	538:559	Therefore, enzymatic production has gained attention as a promising environmentally-friendly alternative to the chemical processes.					
31332537	7	50	theme	α-chitin	1223:1230	arg1	polymorphs					1232:1241	β-chitin and α-chitin polymorphs	1210:1241	β-chitin and α-chitin polymorphs	1210:1241	The use of colloidal α-chitin as substrate showed a substantial improvement of GlcNAc yields, when compared with β-chitin and α-chitin polymorphs.					
31332537	4	51	theme	GlcNAc	595:600	arg1	bioproduction					602:614	the GlcNAc bioproduction	591:614	the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases)	591:755	In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).					
31332537	9	52	theme	GlcNAc	1484:1489	arg1	bioproduction					1491:1503	the GlcNAc bioproduction	1480:1503	the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures	1480:1661	This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures.					
31332537	7	53	theme	colloidal	1108:1116	arg1	α-chitin					1118:1125	colloidal α-chitin	1108:1125	colloidal α-chitin	1108:1125	The use of colloidal α-chitin as substrate showed a substantial improvement of GlcNAc yields, when compared with β-chitin and α-chitin polymorphs.					
31332537	9	54	theme	valuable	1456:1463	arg1	information					1465:1475	valuable information	1456:1475	valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures	1456:1661	This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures.					
31332537	9	55	theme	enzyme	1606:1611	arg1	composition					1622:1632	the enzyme cocktail composition	1602:1632	the enzyme cocktail composition	1602:1632	This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures.					
31332537	7	56	theme	α-chitin	1118:1125	arg1	use					1101:1103	The use	1097:1103	The use of colloidal α-chitin as substrate	1097:1138	The use of colloidal α-chitin as substrate showed a substantial improvement of GlcNAc yields, when compared with β-chitin and α-chitin polymorphs.					
31332537	4	57	dep	endochitinases	676:689	arg1	N-acetyl-glucosaminidases					730:754	N-acetyl-glucosaminidases	730:754	N-acetyl-glucosaminidases	730:754	In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).					
31332537	4	57	dep	endochitinases	676:689	arg1	chitobiosidases					710:724	chitobiosidases	710:724	chitobiosidases	710:724	In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).					
31332537	2	58	theme	chemical	291:298	arg1	hydrolysis					300:309	the chemical hydrolysis	287:309	the chemical hydrolysis of chitin	287:319	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
31332537	9	59	theme	cocktail	1613:1620	arg1	composition					1622:1632	the enzyme cocktail composition	1602:1632	the enzyme cocktail composition	1602:1632	This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures.					
31332537	6	60	theme	Hydrolysis	934:943	arg1	parameters					945:954	Hydrolysis parameters	934:954	Hydrolysis parameters	934:954	Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6 h.					
31332537	4	61	contain	containing	665:674	arg2	exochitinases					695:707	exochitinases	695:707	exochitinases	695:707	In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).					
31332537	4	61	contain	containing	665:674	arg2	endochitinases					676:689	endochitinases	676:689	endochitinases	676:689	In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).					
31332537	4	61	contain	containing	665:674	arg1	cocktail					656:663	an enzyme cocktail	646:663	an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases)	646:755	In this study we demonstrate the GlcNAc bioproduction from colloidal α-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases).					
31332537	1	62	theme	biotechnological	218:233	arg1	applications					235:246	biotechnological applications	218:246	biotechnological applications	218:246	N-acetyl-D-glucosamine (GlcNAc) is an important amino-monosaccharide with great potential for biotechnological applications.					
31332537	8	63	theme	depolymerized	1414:1426	arg1	chains					1428:1433	the depolymerized chains	1410:1433	the depolymerized chains	1410:1433	Such result is directly related to a significant decrease in crystallinity and viscosity from natural α-chitin, providing the chitinase with greater accessibility to the depolymerized chains.					
31332537	0	64	theme	enzyme	71:76	arg1	cocktail					78:85	an enzyme cocktail	68:85	an enzyme cocktail	68:85	Bioproduction of N-acetyl-glucosamine from colloidal α-chitin using an enzyme cocktail produced by Aeromonas caviae CHZ306.					
31332537	2	65	theme	low	403:405	arg1	yields					407:412	low yields	403:412	low yields	403:412	It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs.					
30125969	7	0	theme	straw	1028:1032	arg1	cultivars					1034:1042	the different straw cultivars	1014:1042	the different straw cultivars	1014:1042	Among the different straw cultivars, the extent of lignin degradation varied greatly (47% to 93.5%).					
30125969	10	1	theme	degradation	1507:1517	arg1	pronounced					1555:1564	pronounced	1555:1564	pronounced	1555:1564	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	10	1	theme	degradation	1507:1517	arg1	extent					1490:1495	The extent	1486:1495	The extent of lignin degradation caused by fungal treatment	1486:1544	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	9	2	dep	CVs	1449:1451	arg1	%					1458:1458	43.2%	1454:1458	43.2%	1454:1458	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	9	2	dep	CVs	1449:1451	arg1	%					1471:1471	29.1%	1467:1471	29.1%	1467:1471	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	3	3	with	comparison	547:556	arg1	straw					567:571	the straw	563:571	the straw harvested at an earlier stage (MS1; 70.7%)	563:614	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	3	4	theme	pronounced	475:484	arg1	degradation					486:496	a more pronounced degradation	468:496	a more pronounced degradation of lignin	468:506	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	2	5	theme	Different	254:262	arg1	CV					275:276	CV	275:276	CV	275:276	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	2	5	theme	Different	254:262	arg1	cultivars					264:272	Different cultivars	254:272	Different cultivars (CV) of wheat straw at different maturity stages (MS)	254:326	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	8	6	dep	significant	1130:1140	arg1	<					1145:1145	P < 0.001	1143:1151	P < 0.001	1143:1151	This may explain the significant (P < 0.001) effect of cultivar on the IVGP of the fungal-treated straws.					
30125969	11	7	theme	straw	1726:1730	arg1	types					1732:1736	various straw types	1718:1736	various straw types	1718:1736	Both fungi were capable of improving the IVGP of various straw types.					
30125969	6	8	theme	different	953:961	arg1	MS					963:964	different MS	953:964	different MS	953:964	There was no effect of different MS on the IVGP of the fungus-treated straws.					
30125969	12	9	theme	©	1739:1739	arg1	2018					1741:1744	© 2018	1739:1744	© 2018	1739:1744	© 2018 The Authors.					
30125969	2	10	theme	wheat	282:286	arg1	straw					288:292	wheat straw	282:292	wheat straw	282:292	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	3	11	dep	straw	525:529	arg1	MS3					532:534	MS3	532:534	MS3; 89.0%	532:541	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	4	12	theme	mature	781:786	arg1	straw					788:792	more mature straw	776:792	more mature straw	776:792	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	3	13	dep	MS3	532:534	arg1	%					541:541	89.0%	537:541	MS3; 89.0%	532:541	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	9	14	with	capable	1270:1276	arg1	CS					1377:1378	CS	1377:1378	CS	1377:1378	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	1	15	theme	ruminal	120:126	arg1	degradability					128:140	the ruminal degradability	116:140	the ruminal degradability of various wheat straw types	116:169	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	8	16	theme	fungal-treated	1192:1205	arg1	straws					1207:1212	the fungal-treated straws	1188:1212	the fungal-treated straws	1188:1212	This may explain the significant (P < 0.001) effect of cultivar on the IVGP of the fungal-treated straws.					
30125969	6	17	theme	straws	1000:1005	arg1	IVGP					973:976	the IVGP	969:976	the IVGP of the fungus-treated straws	969:1005	There was no effect of different MS on the IVGP of the fungus-treated straws.					
30125969	4	18	theme	greater	825:831	arg1	extent					833:838	a greater extent	823:838	a greater extent	823:838	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	7	19	theme	different	1018:1026	arg1	cultivars					1034:1042	the different straw cultivars	1014:1042	the different straw cultivars	1014:1042	Among the different straw cultivars, the extent of lignin degradation varied greatly (47% to 93.5%).					
30125969	1	20	theme	wheat	153:157	arg1	types					165:169	various wheat straw types	145:169	various wheat straw types	145:169	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	1	21	dep	BACKGROUND	81:90	arg1	investigated					103:114	investigated	103:114	investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE)	103:251	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	10	22	theme	different	1648:1656	arg1	cultivars					1658:1666	different cultivars	1648:1666	different cultivars	1648:1666	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	1	23	theme	types	165:169	arg1	degradability					128:140	the ruminal degradability	116:140	the ruminal degradability of various wheat straw types	116:169	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	13	24	dep	Sons	1832:1835	arg1	Ltd					1837:1839	Ltd	1837:1839	Ltd	1837:1839	Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
30125969	4	25	theme	gas	651:653	arg1	chromatography					655:668	gas chromatography	651:668	gas chromatography	651:668	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	13	26	theme	Wiley	1824:1828	arg1	Sons					1832:1835	John Wiley & Sons Ltd	1819:1839	John Wiley & Sons Ltd on behalf of Society of Chemical Industry	1819:1881	Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
30125969	3	27	dep	RESULTS	442:448	arg1	Both					450:453	Both	450:453	Both	450:453	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	4	28	theme	Quantitative	617:628	arg1	pyrolysis					630:638	Quantitative pyrolysis	617:638	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS	617:750	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	13	29	theme	John	1819:1822	arg1	Sons					1832:1835	John Wiley & Sons Ltd	1819:1839	John Wiley & Sons Ltd on behalf of Society of Chemical Industry	1819:1881	Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
30125969	13	30	theme	&	1830:1830	arg1	Sons					1832:1835	John Wiley & Sons Ltd	1819:1839	John Wiley & Sons Ltd on behalf of Society of Chemical Industry	1819:1881	Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
30125969	2	31	theme	gas	419:421	arg1	IVGP					435:438	IVGP	435:438	IVGP	435:438	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	2	31	theme	gas	419:421	arg1	production					423:432	in vitro gas production	410:432	in vitro gas production (IVGP)	410:439	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	0	32	theme	various	35:41	arg1	types					55:59	various wheat straw types	35:59	various wheat straw types	35:59	Improving ruminal digestibility of various wheat straw types by white-rot fungi.					
30125969	9	33	theme	types	1313:1317	arg1	IVGP					1295:1298	the IVGP	1291:1298	the IVGP of all straw types	1291:1317	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	2	34	theme	in	410:411	arg1	IVGP					435:438	IVGP	435:438	IVGP	435:438	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	2	34	theme	in	410:411	arg1	production					423:432	in vitro gas production	410:432	in vitro gas production (IVGP)	410:439	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	4	35	theme	C	702:702	arg1	lignin					704:709	13 C lignin	699:709	13 C lignin	699:709	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	4	35	theme	C	702:702	arg1	standard					726:733	standard	726:733	standard	726:733	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	4	36	dep	standard	726:733	arg1	Py-GC/MS					743:750	13 C-IS Py-GC/MS	735:750	an internal standard 13 C-IS Py-GC/MS	714:750	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	7	37	dep	%	1105:1105	arg1	to					1098:1099	to	1098:1099	to	1098:1099	Among the different straw cultivars, the extent of lignin degradation varied greatly (47% to 93.5%).					
30125969	7	38	theme	degradation	1066:1076	arg1	extent					1049:1054	the extent	1045:1054	the extent of lignin degradation	1045:1076	Among the different straw cultivars, the extent of lignin degradation varied greatly (47% to 93.5%).					
30125969	2	39	theme	different	297:305	arg1	MS					324:325	MS	324:325	MS	324:325	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	2	39	theme	different	297:305	arg1	stages					316:321	different maturity stages	297:321	different maturity stages (MS)	297:326	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	10	40	dep	CONCLUSION	1475:1484	arg1	pronounced					1555:1564	pronounced	1555:1564	pronounced	1555:1564	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	10	40	dep	CONCLUSION	1475:1484	arg1	extent					1490:1495	The extent	1486:1495	The extent of lignin degradation caused by fungal treatment	1486:1544	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	12	41	dep	Authors	1750:1756	arg1	2018					1741:1744	© 2018	1739:1744	© 2018	1739:1744	© 2018 The Authors.					
30125969	1	42	theme	Lentinula	231:239	arg1	LE					249:250	LE	249:250	LE	249:250	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	1	42	theme	Lentinula	231:239	arg1	edodes					241:246	Lentinula edodes	231:246	Lentinula edodes (LE)	231:251	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	5	43	dep	MS1	905:907	arg1	%					913:913	8.3%	910:913	8.3%	910:913	In contrast, cellulose was less degraded in MS3, as compared to MS1 (8.3% versus 14.6%).					
30125969	5	43	dep	MS1	905:907	arg1	%					926:926	14.6%	922:926	14.6%	922:926	In contrast, cellulose was less degraded in MS3, as compared to MS1 (8.3% versus 14.6%).					
30125969	11	44	theme	various	1718:1724	arg1	types					1732:1736	various straw types	1718:1736	various straw types	1718:1736	Both fungi were capable of improving the IVGP of various straw types.					
30125969	10	45	theme	lignin	1500:1505	arg1	degradation					1507:1517	lignin degradation	1500:1517	lignin degradation caused by fungal treatment	1500:1544	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	2	46	theme	straw	288:292	arg1	CV					275:276	CV	275:276	CV	275:276	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	2	46	theme	straw	288:292	arg1	cultivars					264:272	Different cultivars	254:272	Different cultivars (CV) of wheat straw at different maturity stages (MS)	254:326	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	4	47	theme	C-IS	738:741	arg1	Py-GC/MS					743:750	13 C-IS Py-GC/MS	735:750	an internal standard 13 C-IS Py-GC/MS	714:750	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	2	48	from	stages	316:321	arg1	CV					275:276	CV	275:276	CV	275:276	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	2	48	from	stages	316:321	arg1	cultivars					264:272	Different cultivars	254:272	Different cultivars (CV) of wheat straw at different maturity stages (MS)	254:326	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	9	49	theme	%	1326:1326	arg1	%					1335:1335	15.3% to 47.6%	1322:1335	15.3% to 47.6%, (as compared to untreated straw)	1322:1369	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	0	50	theme	straw	49:53	arg1	types					55:59	various wheat straw types	35:59	various wheat straw types	35:59	Improving ruminal digestibility of various wheat straw types by white-rot fungi.					
30125969	9	51	theme	untreated	1354:1362	arg1	straw					1364:1368	untreated straw	1354:1368	untreated straw	1354:1368	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	6	52	theme	MS	963:964	arg1	effect					943:948	no effect	940:948	no effect of different MS on the IVGP of the fungus-treated straws	940:1005	There was no effect of different MS on the IVGP of the fungus-treated straws.					
30125969	10	53	theme	fungal	1529:1534	arg1	treatment					1536:1544	fungal treatment	1529:1544	fungal treatment	1529:1544	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	3	54	theme	lignin	501:506	arg1	degradation					486:496	a more pronounced degradation	468:496	a more pronounced degradation of lignin	468:506	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	1	55	theme	white-rot	178:186	arg1	subvermispora					208:220	the white-rot fungi Ceriporiopsis subvermispora	174:220	the white-rot fungi Ceriporiopsis subvermispora (CS)	174:225	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	1	55	theme	white-rot	178:186	arg1	CS					223:224	CS	223:224	CS	223:224	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	11	56	theme	types	1732:1736	arg1	IVGP					1710:1713	the IVGP	1706:1713	the IVGP of various straw types	1706:1736	Both fungi were capable of improving the IVGP of various straw types.					
30125969	9	57	dep	MS	1421:1422	arg1	%					1429:1429	33.6%	1425:1429	33.6%	1425:1429	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	9	57	dep	MS	1421:1422	arg1	%					1442:1442	20.4%	1438:1442	20.4%	1438:1442	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	1	58	theme	Ceriporiopsis	194:206	arg1	subvermispora					208:220	the white-rot fungi Ceriporiopsis subvermispora	174:220	the white-rot fungi Ceriporiopsis subvermispora (CS)	174:225	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	1	58	theme	Ceriporiopsis	194:206	arg1	CS					223:224	CS	223:224	CS	223:224	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	9	59	dep	%	1335:1335	arg1	to					1328:1329	to	1328:1329	to	1328:1329	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	9	59	dep	%	1335:1335	arg1	compared					1342:1349	compared	1342:1349	compared to untreated straw	1342:1368	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	3	60	theme	mature	518:523	arg1	straw					525:529	a more mature straw	511:529	a more mature straw (MS3; 89.0%)	511:542	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	8	61	from	effect	1154:1159	arg1	IVGP					1180:1183	the IVGP	1176:1183	the IVGP of the fungal-treated straws	1176:1212	This may explain the significant (P < 0.001) effect of cultivar on the IVGP of the fungal-treated straws.					
30125969	10	62	theme	lignified	1589:1597	arg1	straw					1599:1603	the more mature and lignified straw	1569:1603	the more mature and lignified straw	1569:1603	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	8	63	theme	straws	1207:1212	arg1	IVGP					1180:1183	the IVGP	1176:1183	the IVGP of the fungal-treated straws	1176:1212	This may explain the significant (P < 0.001) effect of cultivar on the IVGP of the fungal-treated straws.					
30125969	10	64	theme	mature	1578:1583	arg1	straw					1599:1603	the more mature and lignified straw	1569:1603	the more mature and lignified straw	1569:1603	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	4	65	from	lignin	766:771	arg1	straw					788:792	more mature straw	776:792	more mature straw	776:792	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	9	66	from	LE	1403:1404	arg1	MS					1421:1422	different MS	1411:1422	different MS (33.6% versus 20.4%)	1411:1443	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	9	66	from	LE	1403:1404	arg1	CVs					1449:1451	CVs	1449:1451	CVs (43.2% versus 29.1%)	1449:1472	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	6	67	theme	fungus-treated	985:998	arg1	straws					1000:1005	the fungus-treated straws	981:1005	the fungus-treated straws	981:1005	There was no effect of different MS on the IVGP of the fungus-treated straws.					
30125969	10	68	theme	variable	1612:1619	arg1	results					1621:1627	variable results	1612:1627	variable results	1612:1627	CONCLUSION The extent of lignin degradation caused by fungal treatment was more pronounced on the more mature and lignified straw, while variable results were obtained with different cultivars.					
30125969	0	69	theme	white-rot	64:72	arg1	fungi					74:78	white-rot fungi	64:78	white-rot fungi	64:78	Improving ruminal digestibility of various wheat straw types by white-rot fungi.					
30125969	13	70	theme	Chemical	1865:1872	arg1	Industry					1874:1881	Chemical Industry	1865:1881	Chemical Industry	1865:1881	Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
30125969	1	71	theme	various	145:151	arg1	types					165:169	various wheat straw types	145:169	various wheat straw types	145:169	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	9	72	theme	different	1411:1419	arg1	MS					1421:1422	different MS	1411:1422	different MS (33.6% versus 20.4%)	1411:1443	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	2	73	dep	in	410:411	arg1	vitro					413:417	vitro	413:417	vitro	413:417	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	1	74	theme	straw	159:163	arg1	types					165:169	various wheat straw types	145:169	various wheat straw types	145:169	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	0	75	theme	ruminal	10:16	arg1	digestibility					18:30	ruminal digestibility	10:30	ruminal digestibility of various wheat straw types by white-rot fungi	10:78	Improving ruminal digestibility of various wheat straw types by white-rot fungi.					
30125969	7	76	theme	%	1096:1096	arg1	%					1105:1105	47% to 93.5%	1094:1105	47% to 93.5%	1094:1105	Among the different straw cultivars, the extent of lignin degradation varied greatly (47% to 93.5%).					
30125969	2	77	theme	chemical	385:392	arg1	composition					394:404	chemical composition	385:404	chemical composition	385:404	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	6	78	from	effect	943:948	arg1	IVGP					973:976	the IVGP	969:976	the IVGP of the fungus-treated straws	969:1005	There was no effect of different MS on the IVGP of the fungus-treated straws.					
30125969	8	79	theme	significant	1130:1140	arg1	effect					1154:1159	the significant (P < 0.001) effect	1126:1159	the significant (P < 0.001) effect of cultivar on the IVGP of the fungal-treated straws	1126:1212	This may explain the significant (P < 0.001) effect of cultivar on the IVGP of the fungal-treated straws.					
30125969	0	80	theme	wheat	43:47	arg1	types					55:59	various wheat straw types	35:59	various wheat straw types	35:59	Improving ruminal digestibility of various wheat straw types by white-rot fungi.					
30125969	3	81	dep	stage	597:601	arg1	MS1					604:606	MS1	604:606	MS1; 70.7%	604:613	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	9	82	theme	straw	1307:1311	arg1	types					1313:1317	all straw types	1303:1317	all straw types	1303:1317	Regardless of the factors tested, both fungi were very capable of improving the IVGP of all straw types by 15.3% to 47.6%, (as compared to untreated straw), with CS performing better than LE - on different MS (33.6% versus 20.4%) and CVs (43.2% versus 29.1%).					
30125969	8	83	theme	P	1143:1143	arg1	<					1145:1145	P < 0.001	1143:1151	P < 0.001	1143:1151	This may explain the significant (P < 0.001) effect of cultivar on the IVGP of the fungal-treated straws.					
30125969	0	84	theme	types	55:59	arg1	digestibility					18:30	ruminal digestibility	10:30	ruminal digestibility of various wheat straw types by white-rot fungi	10:78	Improving ruminal digestibility of various wheat straw types by white-rot fungi.					
30125969	3	85	dep	MS1	604:606	arg1	%					613:613	70.7%	609:613	MS1; 70.7%	604:613	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	1	86	theme	fungi	188:192	arg1	subvermispora					208:220	the white-rot fungi Ceriporiopsis subvermispora	174:220	the white-rot fungi Ceriporiopsis subvermispora (CS)	174:225	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	1	86	theme	fungi	188:192	arg1	CS					223:224	CS	223:224	CS	223:224	BACKGROUND This study investigated the ruminal degradability of various wheat straw types by the white-rot fungi Ceriporiopsis subvermispora (CS) and Lentinula edodes (LE).					
30125969	7	87	theme	lignin	1059:1064	arg1	degradation					1066:1076	lignin degradation	1059:1076	lignin degradation	1059:1076	Among the different straw cultivars, the extent of lignin degradation varied greatly (47% to 93.5%).					
30125969	4	88	theme	13	699:700	arg1	C					702:702	C	702:702	C	702:702	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	2	89	theme	maturity	307:314	arg1	MS					324:325	MS	324:325	MS	324:325	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	2	89	theme	maturity	307:314	arg1	stages					316:321	different maturity stages	297:321	different maturity stages (MS)	297:326	Different cultivars (CV) of wheat straw at different maturity stages (MS) were treated with the fungi for 7 weeks and assessed for chemical composition and in vitro gas production (IVGP).					
30125969	4	90	theme	mass	674:677	arg1	spectrometry					679:690	mass spectrometry	674:690	mass spectrometry	674:690	Quantitative pyrolysis coupled to gas chromatography and mass spectrometry, using 13 C lignin as an internal standard 13 C-IS Py-GC/MS revealed that lignin in more mature straw was degraded and modified to a greater extent.					
30125969	7	91	dep	varied	1078:1083	arg1	%					1105:1105	47% to 93.5%	1094:1105	47% to 93.5%	1094:1105	Among the different straw cultivars, the extent of lignin degradation varied greatly (47% to 93.5%).					
30125969	3	92	theme	earlier	589:595	arg1	stage					597:601	an earlier stage	586:601	an earlier stage (MS1; 70.7%)	586:614	RESULTS Both fungi showed a more pronounced degradation of lignin on a more mature straw (MS3; 89.0%) in comparison with the straw harvested at an earlier stage (MS1; 70.7%).					
30125969	8	93	theme	cultivar	1164:1171	arg1	effect					1154:1159	the significant (P < 0.001) effect	1126:1159	the significant (P < 0.001) effect of cultivar on the IVGP of the fungal-treated straws	1126:1212	This may explain the significant (P < 0.001) effect of cultivar on the IVGP of the fungal-treated straws.					
30125969	13	94	theme	Science	1774:1780	arg1	Journal					1759:1765	Journal	1759:1765	Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.	1759:1882	Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.					
30467374	6	0	theme	correlation	1048:1058	arg1	analysis					1060:1067	correlation analysis	1048:1067	correlation analysis	1048:1067	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	6	1	theme	amylose	1230:1236	arg1	biosynthesis					1238:1249	high amylose biosynthesis	1225:1249	high amylose biosynthesis	1225:1249	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	3	2	theme	EMS-induced	468:478	arg1	lines					487:491	EMS-induced mutant lines	468:491	EMS-induced mutant lines differing in amylose content	468:520	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	5	3	from	variations	898:907	arg1	acids					918:922	amino acids composition and predicted physicochemical properties	912:975	amino acids composition and predicted physicochemical properties	912:975	A genome-wide survey identified 370 wheat bZIPs, clustered in 11 groups, showing variations in amino acids composition and predicted physicochemical properties.					
30467374	8	4	dep	In	1315:1316	arg1	silico					1318:1323	silico	1318:1323	silico	1318:1323	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	6	5	theme	bZIPs	1211:1215	arg1	role					1217:1220	bZIPs role	1211:1220	bZIPs role in high amylose biosynthesis	1211:1249	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	10	6	theme	in-depth	1564:1571	arg1	analysis					1573:1580	An in-depth analysis	1561:1580	An in-depth analysis of molecular mechanism of novel candidate bZIPs	1561:1628	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	3	7	theme	zipper	537:542	arg1	factors					562:568	basic leucine zipper (bZIP) regulatory factors	523:568	basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis	523:623	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	5	8	theme	genome-wide	819:829	arg1	survey					831:836	A genome-wide survey	817:836	A genome-wide survey	817:836	A genome-wide survey identified 370 wheat bZIPs, clustered in 11 groups, showing variations in amino acids composition and predicted physicochemical properties.					
30467374	3	9	theme	basic	523:527	arg1	bZIP					545:548	bZIP	545:548	bZIP	545:548	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	3	9	theme	basic	523:527	arg1	zipper					537:542	basic leucine zipper	523:542	basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis	523:623	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	1	10	theme	wheat	158:162	arg1	grain					164:168	the wheat grain	154:168	the wheat grain	154:168	Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems.					
30467374	10	11	theme	mechanism	1595:1603	arg1	analysis					1573:1580	An in-depth analysis	1561:1580	An in-depth analysis of molecular mechanism of novel candidate bZIPs	1561:1628	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	3	12	theme	regulatory	551:560	arg1	factors					562:568	basic leucine zipper (bZIP) regulatory factors	523:568	basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis	523:623	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	6	13	theme	negative	1178:1185	arg1	regulators					1187:1196	positive and negative regulators	1165:1196	positive and negative regulators	1165:1196	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	6	13	theme	negative	1178:1185	arg1	bZIP					1159:1162	24 candidate bZIP	1146:1162	24 candidate bZIP (positive and negative regulators)	1146:1197	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	2	14	with	varieties	397:405	arg1	benefits					419:426	health benefits	412:426	health benefits	412:426	The challenge is to develop resistant starch including high amylose wheat varieties with health benefits.					
30467374	8	15	theme	functional	1428:1437	arg1	role					1439:1442	their putative functional role	1413:1442	their putative functional role	1413:1442	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	6	16	theme	positive	1165:1172	arg1	regulators					1187:1196	positive and negative regulators	1165:1196	positive and negative regulators	1165:1196	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	6	16	theme	positive	1165:1172	arg1	bZIP					1159:1162	24 candidate bZIP	1146:1162	24 candidate bZIP (positive and negative regulators)	1146:1197	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	4	17	from	role	755:758	arg1	wheat					789:793	wheat	789:793	wheat	789:793	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	7	18	theme	positive	1258:1265	arg1	role					1267:1270	bZIPs positive role	1252:1270	bZIPs positive role in high amylose biosynthesis	1252:1299	bZIPs positive role in high amylose biosynthesis is not known.					
30467374	6	19	from	role	1217:1220	arg1	biosynthesis					1238:1249	high amylose biosynthesis	1225:1249	high amylose biosynthesis	1225:1249	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	1	20	theme	starch	260:265	arg1	overconsumption					235:249	the overconsumption	231:249	the overconsumption of wheat starch	231:265	Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems.					
30467374	9	21	theme	stress-related	1482:1495	arg1	pathways					1497:1504	stress-related pathways	1482:1504	stress-related pathways	1482:1504	The identified bZIPs are involved in stress-related pathways, flower and seed development, and starch biosynthesis.					
30467374	4	22	theme	transcription	656:668	arg1	factors					670:676	bZIP transcription factors	651:676	bZIP transcription factors	651:676	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	4	22	theme	transcription	656:668	arg1	regulators					686:695	key regulators	682:695	key regulators of starch biosynthesis genes in rice and maize	682:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	8	23	from	rice	1397:1400	arg1	studies					1337:1343	In silico interactome studies	1315:1343	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice	1315:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	4	24	from	maize	738:742	arg1	factors					670:676	bZIP transcription factors	651:676	bZIP transcription factors	651:676	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	4	24	from	maize	738:742	arg1	regulators					686:695	key regulators	682:695	key regulators of starch biosynthesis genes in rice and maize	682:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	0	25	dep	Triticum	100:107	arg1	L.					118:119	Triticum aestivum L.	100:119	Triticum aestivum L.	100:119	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.					
30467374	6	26	from	approaches	984:993	arg1	mutants					1105:1111	contrasting high and low amylose mutants	1072:1111	contrasting high and low amylose mutants	1072:1111	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	4	27	theme	biosynthesis	707:718	arg1	genes					720:724	starch biosynthesis genes	700:724	starch biosynthesis genes in rice and maize	700:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	1	28	theme	nutrition-associated	285:304	arg1	problems					313:320	nutrition-associated health problems	285:320	nutrition-associated health problems	285:320	Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems.					
30467374	4	29	from	rice	729:732	arg1	factors					670:676	bZIP transcription factors	651:676	bZIP transcription factors	651:676	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	4	29	from	rice	729:732	arg1	regulators					686:695	key regulators	682:695	key regulators of starch biosynthesis genes in rice and maize	682:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	0	30	from	biosynthesis	33:44	arg1	mutants					122:128	wheat (Triticum aestivum L.) mutants	93:128	wheat (Triticum aestivum L.) mutants	93:128	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.					
30467374	0	31	theme	wheat	93:97	arg1	mutants					122:128	wheat (Triticum aestivum L.) mutants	93:128	wheat (Triticum aestivum L.) mutants	93:128	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.					
30467374	4	32	from	genes	720:724	arg1	rice					729:732	rice	729:732	rice	729:732	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	4	32	from	genes	720:724	arg1	maize					738:742	maize	738:742	maize	738:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	3	33	dep	approaches	454:463	arg1	advance					438:444	advance	438:444	advance	438:444	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	4	34	theme	starch	700:705	arg1	genes					720:724	starch biosynthesis genes	700:724	starch biosynthesis genes in rice and maize	700:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	10	35	theme	candidate	1614:1622	arg1	bZIPs					1624:1628	novel candidate bZIPs	1608:1628	novel candidate bZIPs	1608:1628	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	0	36	theme	Pivotal	0:6	arg1	role					8:11	Pivotal role	0:11	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.	0:129	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.					
30467374	7	37	theme	high	1275:1278	arg1	biosynthesis					1288:1299	high amylose biosynthesis	1275:1299	high amylose biosynthesis	1275:1299	bZIPs positive role in high amylose biosynthesis is not known.					
30467374	2	38	theme	amylose	383:389	arg1	varieties					397:405	high amylose wheat varieties	378:405	high amylose wheat varieties with health benefits	378:426	The challenge is to develop resistant starch including high amylose wheat varieties with health benefits.					
30467374	4	39	theme	genes	720:724	arg1	factors					670:676	bZIP transcription factors	651:676	bZIP transcription factors	651:676	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	4	39	theme	genes	720:724	arg1	regulators					686:695	key regulators	682:695	key regulators of starch biosynthesis genes in rice and maize	682:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	6	40	theme	whole	1003:1007	arg1	sequencing					1023:1032	whole transcriptome sequencing	1003:1032	whole transcriptome sequencing	1003:1032	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	3	41	theme	amylose	604:610	arg1	biosynthesis					612:623	amylose biosynthesis	604:623	amylose biosynthesis	604:623	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	8	42	theme	candidate	1348:1356	arg1	homologs					1369:1376	candidate wheat bZIP homologs	1348:1376	candidate wheat bZIP homologs in Arabidopsis and rice	1348:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	2	43	theme	resistant	351:359	arg1	starch					361:366	resistant starch	351:366	resistant starch including high amylose wheat varieties with health benefits	351:426	The challenge is to develop resistant starch including high amylose wheat varieties with health benefits.					
30467374	7	44	theme	bZIPs	1252:1256	arg1	role					1267:1270	bZIPs positive role	1252:1270	bZIPs positive role in high amylose biosynthesis	1252:1299	bZIPs positive role in high amylose biosynthesis is not known.					
30467374	2	45	theme	health	412:417	arg1	benefits					419:426	health benefits	412:426	health benefits	412:426	The challenge is to develop resistant starch including high amylose wheat varieties with health benefits.					
30467374	0	46	theme	genome	49:54	arg1	survey					56:61	genome survey	49:61	genome survey	49:61	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.					
30467374	8	47	theme	bZIP	1364:1367	arg1	homologs					1369:1376	candidate wheat bZIP homologs	1348:1376	candidate wheat bZIP homologs in Arabidopsis and rice	1348:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	3	48	theme	genomic	446:452	arg1	approaches					454:463	genomic approaches	446:463	advance genomic approaches	438:463	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	10	49	theme	high	1664:1667	arg1	varieties					1683:1691	raising and improving high amylose wheat varieties	1642:1691	raising and improving high amylose wheat varieties	1642:1691	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	6	50	theme	contrasting	1072:1082	arg1	mutants					1105:1111	contrasting high and low amylose mutants	1072:1111	contrasting high and low amylose mutants	1072:1111	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	3	51	theme	mutant	480:485	arg1	lines					487:491	EMS-induced mutant lines	468:491	EMS-induced mutant lines differing in amylose content	468:520	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	5	52	theme	physicochemical	950:964	arg1	properties					966:975	predicted physicochemical properties	940:975	predicted physicochemical properties	940:975	A genome-wide survey identified 370 wheat bZIPs, clustered in 11 groups, showing variations in amino acids composition and predicted physicochemical properties.					
30467374	6	53	from	analysis	1060:1067	arg1	mutants					1105:1111	contrasting high and low amylose mutants	1072:1111	contrasting high and low amylose mutants	1072:1111	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	6	54	theme	high	1225:1228	arg1	biosynthesis					1238:1249	high amylose biosynthesis	1225:1249	high amylose biosynthesis	1225:1249	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	6	55	from	sequencing	1023:1032	arg1	mutants					1105:1111	contrasting high and low amylose mutants	1072:1111	contrasting high and low amylose mutants	1072:1111	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	3	56	theme	amylose	506:512	arg1	content					514:520	amylose content	506:520	amylose content	506:520	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	1	57	theme	calories	201:208	arg1	source					191:196	an important source	178:196	an important source of calories for humans	178:219	Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems.					
30467374	10	58	theme	wheat	1677:1681	arg1	varieties					1683:1691	raising and improving high amylose wheat varieties	1642:1691	raising and improving high amylose wheat varieties	1642:1691	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	6	59	from	qRT-PCR	1035:1041	arg1	mutants					1105:1111	contrasting high and low amylose mutants	1072:1111	contrasting high and low amylose mutants	1072:1111	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	6	60	theme	parent	1123:1128	arg1	line					1130:1133	their parent line	1117:1133	their parent line	1117:1133	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	3	61	theme	leucine	529:535	arg1	bZIP					545:548	bZIP	545:548	bZIP	545:548	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	3	61	theme	leucine	529:535	arg1	zipper					537:542	basic leucine zipper	523:542	basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis	523:623	Adapting advance genomic approaches in EMS-induced mutant lines differing in amylose content, basic leucine zipper (bZIP) regulatory factors that may play role in controlling amylose biosynthesis were identified in wheat.					
30467374	8	62	from	Arabidopsis	1381:1391	arg1	studies					1337:1343	In silico interactome studies	1315:1343	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice	1315:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	5	63	theme	wheat	853:857	arg1	bZIPs					859:863	370 wheat bZIPs	849:863	370 wheat bZIPs	849:863	A genome-wide survey identified 370 wheat bZIPs, clustered in 11 groups, showing variations in amino acids composition and predicted physicochemical properties.					
30467374	8	64	from	homologs	1369:1376	arg1	rice					1397:1400	rice	1397:1400	rice	1397:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	8	64	from	homologs	1369:1376	arg1	Arabidopsis					1381:1391	Arabidopsis	1381:1391	Arabidopsis	1381:1391	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	1	65	theme	grain	164:168	arg1	%					149:149	70%	147:149	70% of the wheat grain	147:168	Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems.					
30467374	1	65	theme	grain	164:168	arg1	grain					164:168	the wheat grain	154:168	the wheat grain	154:168	Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems.					
30467374	8	66	theme	putative	1419:1426	arg1	role					1439:1442	their putative functional role	1413:1442	their putative functional role	1413:1442	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	10	67	theme	molecular	1585:1593	arg1	mechanism					1595:1603	molecular mechanism	1585:1603	molecular mechanism of novel candidate bZIPs	1585:1628	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	9	68	theme	identified	1449:1458	arg1	bZIPs					1460:1464	The identified bZIPs	1445:1464	The identified bZIPs	1445:1464	The identified bZIPs are involved in stress-related pathways, flower and seed development, and starch biosynthesis.					
30467374	5	69	dep	acids	918:922	arg1	composition					924:934	composition	924:934	composition	924:934	A genome-wide survey identified 370 wheat bZIPs, clustered in 11 groups, showing variations in amino acids composition and predicted physicochemical properties.					
30467374	5	69	dep	acids	918:922	arg1	properties					966:975	predicted physicochemical properties	940:975	predicted physicochemical properties	940:975	A genome-wide survey identified 370 wheat bZIPs, clustered in 11 groups, showing variations in amino acids composition and predicted physicochemical properties.					
30467374	4	70	theme	key	682:684	arg1	factors					670:676	bZIP transcription factors	651:676	bZIP transcription factors	651:676	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	4	70	theme	key	682:684	arg1	regulators					686:695	key regulators	682:695	key regulators of starch biosynthesis genes in rice and maize	682:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	6	71	theme	candidate	1149:1157	arg1	regulators					1187:1196	positive and negative regulators	1165:1196	positive and negative regulators	1165:1196	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	6	71	theme	candidate	1149:1157	arg1	bZIP					1159:1162	24 candidate bZIP	1146:1162	24 candidate bZIP (positive and negative regulators)	1146:1197	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	0	72	theme	transcriptome	67:79	arg1	analysis					81:88	transcriptome analysis	67:88	transcriptome analysis	67:88	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.					
30467374	0	73	from	role	8:11	arg1	biosynthesis					33:44	amylose biosynthesis	25:44	amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants	25:128	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.					
30467374	4	74	theme	bZIP	651:654	arg1	factors					670:676	bZIP transcription factors	651:676	bZIP transcription factors	651:676	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	4	74	theme	bZIP	651:654	arg1	regulators					686:695	key regulators	682:695	key regulators of starch biosynthesis genes in rice and maize	682:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	6	75	theme	amylose	1097:1103	arg1	mutants					1105:1111	contrasting high and low amylose mutants	1072:1111	contrasting high and low amylose mutants	1072:1111	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	6	76	from	line	1130:1133	arg1	mutants					1105:1111	contrasting high and low amylose mutants	1072:1111	contrasting high and low amylose mutants	1072:1111	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	1	77	theme	health	306:311	arg1	problems					313:320	nutrition-associated health problems	285:320	nutrition-associated health problems	285:320	Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems.					
30467374	5	78	theme	amino	912:916	arg1	acids					918:922	amino acids composition and predicted physicochemical properties	912:975	amino acids composition and predicted physicochemical properties	912:975	A genome-wide survey identified 370 wheat bZIPs, clustered in 11 groups, showing variations in amino acids composition and predicted physicochemical properties.					
30467374	7	79	theme	amylose	1280:1286	arg1	biosynthesis					1288:1299	high amylose biosynthesis	1275:1299	high amylose biosynthesis	1275:1299	bZIPs positive role in high amylose biosynthesis is not known.					
30467374	10	80	theme	bZIPs	1624:1628	arg1	mechanism					1595:1603	molecular mechanism	1585:1603	molecular mechanism of novel candidate bZIPs	1585:1628	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	2	81	theme	wheat	391:395	arg1	varieties					397:405	high amylose wheat varieties	378:405	high amylose wheat varieties with health benefits	378:426	The challenge is to develop resistant starch including high amylose wheat varieties with health benefits.					
30467374	8	82	theme	In	1315:1316	arg1	studies					1337:1343	In silico interactome studies	1315:1343	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice	1315:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	10	83	theme	novel	1608:1612	arg1	bZIPs					1624:1628	novel candidate bZIPs	1608:1628	novel candidate bZIPs	1608:1628	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	0	84	theme	bZIPs	16:20	arg1	role					8:11	Pivotal role	0:11	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.	0:129	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.					
30467374	9	85	theme	starch	1540:1545	arg1	biosynthesis					1547:1558	starch biosynthesis	1540:1558	starch biosynthesis	1540:1558	The identified bZIPs are involved in stress-related pathways, flower and seed development, and starch biosynthesis.					
30467374	6	86	theme	transcriptome	1009:1021	arg1	sequencing					1023:1032	whole transcriptome sequencing	1003:1032	whole transcriptome sequencing	1003:1032	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	2	87	theme	high	378:381	arg1	varieties					397:405	high amylose wheat varieties	378:405	high amylose wheat varieties with health benefits	378:426	The challenge is to develop resistant starch including high amylose wheat varieties with health benefits.					
30467374	9	88	theme	seed	1518:1521	arg1	development					1523:1533	seed development	1518:1533	seed development	1518:1533	The identified bZIPs are involved in stress-related pathways, flower and seed development, and starch biosynthesis.					
30467374	8	89	theme	interactome	1325:1335	arg1	studies					1337:1343	In silico interactome studies	1315:1343	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice	1315:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	10	90	theme	raising	1642:1648	arg1	varieties					1683:1691	raising and improving high amylose wheat varieties	1642:1691	raising and improving high amylose wheat varieties	1642:1691	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	0	91	theme	amylose	25:31	arg1	biosynthesis					33:44	amylose biosynthesis	25:44	amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants	25:128	Pivotal role of bZIPs in amylose biosynthesis by genome survey and transcriptome analysis in wheat (Triticum aestivum L.) mutants.					
30467374	6	92	theme	low	1093:1095	arg1	mutants					1105:1111	contrasting high and low amylose mutants	1072:1111	contrasting high and low amylose mutants	1072:1111	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	8	93	theme	wheat	1358:1362	arg1	homologs					1369:1376	candidate wheat bZIP homologs	1348:1376	candidate wheat bZIP homologs in Arabidopsis and rice	1348:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	10	94	theme	amylose	1669:1675	arg1	varieties					1683:1691	raising and improving high amylose wheat varieties	1642:1691	raising and improving high amylose wheat varieties	1642:1691	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	4	95	from	regulators	686:695	arg1	rice					729:732	rice	729:732	rice	729:732	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	4	95	from	regulators	686:695	arg1	maize					738:742	maize	738:742	maize	738:742	bZIP transcription factors are key regulators of starch biosynthesis genes in rice and maize, but their role in regulating these genes in wheat is poorly understood.					
30467374	1	96	theme	wheat	254:258	arg1	starch					260:265	wheat starch	254:265	wheat starch	254:265	Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems.					
30467374	7	97	from	role	1267:1270	arg1	biosynthesis					1288:1299	high amylose biosynthesis	1275:1299	high amylose biosynthesis	1275:1299	bZIPs positive role in high amylose biosynthesis is not known.					
30467374	6	98	theme	high	1084:1087	arg1	mutants					1105:1111	contrasting high and low amylose mutants	1072:1111	contrasting high and low amylose mutants	1072:1111	Three approaches namely, whole transcriptome sequencing, qRT-PCR, and correlation analysis in contrasting high and low amylose mutants and their parent line identified 24 candidate bZIP (positive and negative regulators), suggesting bZIPs role in high amylose biosynthesis.					
30467374	8	99	theme	homologs	1369:1376	arg1	studies					1337:1343	In silico interactome studies	1315:1343	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice	1315:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	10	100	theme	improving	1654:1662	arg1	varieties					1683:1691	raising and improving high amylose wheat varieties	1642:1691	raising and improving high amylose wheat varieties	1642:1691	An in-depth analysis of molecular mechanism of novel candidate bZIPs may help in raising and improving high amylose wheat varieties.					
30467374	5	101	theme	predicted	940:948	arg1	properties					966:975	predicted physicochemical properties	940:975	predicted physicochemical properties	940:975	A genome-wide survey identified 370 wheat bZIPs, clustered in 11 groups, showing variations in amino acids composition and predicted physicochemical properties.					
30467374	8	102	from	studies	1337:1343	arg1	rice					1397:1400	rice	1397:1400	rice	1397:1400	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	8	102	from	studies	1337:1343	arg1	Arabidopsis					1381:1391	Arabidopsis	1381:1391	Arabidopsis	1381:1391	In silico interactome studies of candidate wheat bZIP homologs in Arabidopsis and rice identified their putative functional role.					
30467374	1	103	theme	important	181:189	arg1	source					191:196	an important source	178:196	an important source of calories for humans	178:219	Starch makes up 70% of the wheat grain, and is an important source of calories for humans, however, the overconsumption of wheat starch may contribute to nutrition-associated health problems.					
31232647	1	0	theme	osteoarthritis	358:371	arg1	degeneration					373:384	osteoarthritis degeneration	358:384	osteoarthritis degeneration	358:384	It has been experimentally proposed that the discrete regions of articular cartilage, along with different subchondral bone tissues, known as the bone-cartilage unit, are biomechanically altered during osteoarthritis degeneration.					
31232647	4	1	theme	permeability	955:966	arg1	increase					988:995	the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase	897:995	the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis	897:1041	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	2	2	from	changes	452:458	arg1	parameters					479:488	the multiphasic parameters	463:488	the multiphasic parameters	463:488	However, a computational framework capturing all of the dominant changes in the multiphasic parameters has not yet been developed.					
31232647	1	3	theme	bone	275:278	arg1	tissues					280:286	different subchondral bone tissues	253:286	different subchondral bone tissues	253:286	It has been experimentally proposed that the discrete regions of articular cartilage, along with different subchondral bone tissues, known as the bone-cartilage unit, are biomechanically altered during osteoarthritis degeneration.					
31232647	5	4	theme	100 kPa	1228:1234	arg1	maximum					1217:1223	a maximum	1215:1223	a maximum of 100 kPa	1215:1234	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	6	5	theme	composition-based	1394:1410	arg1	studies					1412:1418	the osteoarthritis composition-based studies	1375:1418	the osteoarthritis composition-based studies	1375:1418	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	6	6	theme	calcified	1476:1484	arg1	cartilage					1486:1494	the calcified cartilage	1472:1494	the calcified cartilage	1472:1494	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	3	7	theme	new	542:544	arg1	model					561:565	a new finite element model	540:565	a new finite element model of the bone-cartilage unit	540:592	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	5	8	from	importance	1067:1076	arg1	distribution					1115:1126	fluid distribution	1109:1126	fluid distribution within the bone-cartilage units	1109:1158	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	7	9	theme	unit	1921:1924	arg1	model					1926:1930	the proposed bone-cartilage unit model	1893:1930	the proposed bone-cartilage unit model	1893:1930	To conclude, the new insights provided by the results reveal the significance of the multiphasic osteoarthritis simulation and demonstrate the functionality of the proposed bone-cartilage unit model.					
31232647	6	10	theme	osteoarthritis	1379:1392	arg1	studies					1412:1418	the osteoarthritis composition-based studies	1375:1418	the osteoarthritis composition-based studies	1375:1418	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	4	11	theme	depletion	914:922	arg1	increase					988:995	the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase	897:995	the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis	897:1041	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	6	12	theme	relative	1567:1574	arg1	contributions					1581:1593	relative load contributions	1567:1593	relative load contributions of articular cartilage constituents	1567:1629	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	6	13	theme	70 kPa	1535:1540	arg1	change					1525:1530	a maximum change	1515:1530	a maximum change of 70 kPa in stress	1515:1550	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	6	14	theme	cartilage	1486:1494	arg1	role					1464:1467	the significant biomechanical role	1434:1467	the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration	1434:1730	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	7	15	theme	model	1926:1930	arg1	functionality					1876:1888	the functionality	1872:1888	the functionality of the proposed bone-cartilage unit model	1872:1930	To conclude, the new insights provided by the results reveal the significance of the multiphasic osteoarthritis simulation and demonstrate the functionality of the proposed bone-cartilage unit model.					
31232647	3	16	theme	bone-cartilage	574:587	arg1	unit					589:592	the bone-cartilage unit	570:592	the bone-cartilage unit	570:592	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	0	17	theme	unconfined	126:135	arg1	tests					149:153	unconfined compression tests	126:153	unconfined compression tests	126:153	Computational simulation of the multiphasic degeneration of the bone-cartilage unit during osteoarthritis via indentation and unconfined compression tests.					
31232647	3	18	theme	osteoarthritis	778:791	arg1	degeneration					793:804	osteoarthritis degeneration	778:804	osteoarthritis degeneration by indentation and unconfined compression tests	778:852	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	4	19	theme	collagen	925:932	arg1	softening					944:952	collagen fibrillar softening	925:952	collagen fibrillar softening	925:952	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	1	20	theme	articular	221:229	arg1	cartilage					231:239	articular cartilage	221:239	articular cartilage	221:239	It has been experimentally proposed that the discrete regions of articular cartilage, along with different subchondral bone tissues, known as the bone-cartilage unit, are biomechanically altered during osteoarthritis degeneration.					
31232647	5	21	theme	subchondral	1081:1091	arg1	tissues					1098:1104	subchondral bone tissues	1081:1104	subchondral bone tissues	1081:1104	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	3	22	theme	dominant	751:758	arg1	parameters					760:769	the dominant parameters	747:769	the dominant parameters	747:769	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	7	23	theme	bone-cartilage	1906:1919	arg1	model					1926:1930	the proposed bone-cartilage unit model	1893:1930	the proposed bone-cartilage unit model	1893:1930	To conclude, the new insights provided by the results reveal the significance of the multiphasic osteoarthritis simulation and demonstrate the functionality of the proposed bone-cartilage unit model.					
31232647	5	24	theme	bone	1093:1096	arg1	tissues					1098:1104	subchondral bone tissues	1081:1104	subchondral bone tissues	1081:1104	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	6	25	theme	osmotic	1667:1673	arg1	pressure					1675:1682	the osmotic pressure	1663:1682	the osmotic pressure	1663:1682	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	5	26	theme	tissues	1098:1104	arg1	importance					1067:1076	the importance	1063:1076	the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units	1063:1158	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	4	27	theme	softening	944:952	arg1	increase					988:995	the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase	897:995	the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis	897:1041	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	6	28	theme	maximum	1517:1523	arg1	change					1525:1530	a maximum change	1515:1530	a maximum change of 70 kPa in stress	1515:1550	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	6	29	theme	load	1576:1579	arg1	contributions					1581:1593	relative load contributions	1567:1593	relative load contributions of articular cartilage constituents	1567:1629	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	7	30	theme	multiphasic	1818:1828	arg1	simulation					1845:1854	the multiphasic osteoarthritis simulation	1814:1854	the multiphasic osteoarthritis simulation	1814:1854	To conclude, the new insights provided by the results reveal the significance of the multiphasic osteoarthritis simulation and demonstrate the functionality of the proposed bone-cartilage unit model.					
31232647	1	31	theme	discrete	201:208	arg1	regions					210:216	the discrete regions	197:216	the discrete regions	197:216	It has been experimentally proposed that the discrete regions of articular cartilage, along with different subchondral bone tissues, known as the bone-cartilage unit, are biomechanically altered during osteoarthritis degeneration.					
31232647	0	32	theme	Computational	0:12	arg1	simulation					14:23	Computational simulation	0:23	Computational simulation of the multiphasic degeneration of the bone-cartilage unit during osteoarthritis via indentation and unconfined compression tests.	0:154	Computational simulation of the multiphasic degeneration of the bone-cartilage unit during osteoarthritis via indentation and unconfined compression tests.					
31232647	4	33	theme	fibrillar	934:942	arg1	softening					944:952	collagen fibrillar softening	925:952	collagen fibrillar softening	925:952	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	3	34	theme	several	607:613	arg1	models					686:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	6	35	from	change	1525:1530	arg1	stress					1545:1550	stress	1545:1550	stress	1545:1550	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	2	36	theme	multiphasic	467:477	arg1	parameters					479:488	the multiphasic parameters	463:488	the multiphasic parameters	463:488	However, a computational framework capturing all of the dominant changes in the multiphasic parameters has not yet been developed.					
31232647	5	37	theme	fluid	1109:1113	arg1	distribution					1115:1126	fluid distribution	1109:1126	fluid distribution within the bone-cartilage units	1109:1158	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	4	38	theme	non-advanced	1015:1026	arg1	osteoarthritis					1028:1041	approximately non-advanced osteoarthritis	1001:1041	approximately non-advanced osteoarthritis	1001:1041	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	7	39	theme	proposed	1897:1904	arg1	model					1926:1930	the proposed bone-cartilage unit model	1893:1930	the proposed bone-cartilage unit model	1893:1930	To conclude, the new insights provided by the results reveal the significance of the multiphasic osteoarthritis simulation and demonstrate the functionality of the proposed bone-cartilage unit model.					
31232647	3	40	theme	unit	589:592	arg1	model					561:565	a new finite element model	540:565	a new finite element model of the bone-cartilage unit	540:592	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	1	41	theme	bone-cartilage	302:315	arg1	unit					317:320	the bone-cartilage unit	298:320	the bone-cartilage unit	298:320	It has been experimentally proposed that the discrete regions of articular cartilage, along with different subchondral bone tissues, known as the bone-cartilage unit, are biomechanically altered during osteoarthritis degeneration.					
31232647	0	42	theme	compression	137:147	arg1	tests					149:153	unconfined compression tests	126:153	unconfined compression tests	126:153	Computational simulation of the multiphasic degeneration of the bone-cartilage unit during osteoarthritis via indentation and unconfined compression tests.					
31232647	5	43	theme	subchondral	1287:1297	arg1	bones					1299:1303	subchondral bones	1287:1303	subchondral bones	1287:1303	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	7	44	theme	osteoarthritis	1830:1843	arg1	simulation					1845:1854	the multiphasic osteoarthritis simulation	1814:1854	the multiphasic osteoarthritis simulation	1814:1854	To conclude, the new insights provided by the results reveal the significance of the multiphasic osteoarthritis simulation and demonstrate the functionality of the proposed bone-cartilage unit model.					
31232647	5	45	theme	bone-cartilage	1139:1152	arg1	units					1154:1158	the bone-cartilage units	1135:1158	the bone-cartilage units	1135:1158	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	2	46	theme	computational	398:410	arg1	framework					412:420	a computational framework	396:420	a computational framework capturing all of the dominant changes in the multiphasic parameters	396:488	However, a computational framework capturing all of the dominant changes in the multiphasic parameters has not yet been developed.					
31232647	0	47	theme	degeneration	44:55	arg1	simulation					14:23	Computational simulation	0:23	Computational simulation of the multiphasic degeneration of the bone-cartilage unit during osteoarthritis via indentation and unconfined compression tests.	0:154	Computational simulation of the multiphasic degeneration of the bone-cartilage unit during osteoarthritis via indentation and unconfined compression tests.					
31232647	7	48	theme	new	1750:1752	arg1	insights					1754:1761	the new insights	1746:1761	the new insights provided by the results	1746:1785	To conclude, the new insights provided by the results reveal the significance of the multiphasic osteoarthritis simulation and demonstrate the functionality of the proposed bone-cartilage unit model.					
31232647	6	49	theme	biomechanical	1450:1462	arg1	role					1464:1467	the significant biomechanical role	1434:1467	the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration	1434:1730	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	1	50	theme	cartilage	231:239	arg1	regions					210:216	the discrete regions	197:216	the discrete regions	197:216	It has been experimentally proposed that the discrete regions of articular cartilage, along with different subchondral bone tissues, known as the bone-cartilage unit, are biomechanically altered during osteoarthritis degeneration.					
31232647	3	51	theme	nonlinear	626:634	arg1	models					686:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	2	52	theme	dominant	443:450	arg1	changes					452:458	the dominant changes	439:458	the dominant changes in the multiphasic parameters	439:488	However, a computational framework capturing all of the dominant changes in the multiphasic parameters has not yet been developed.					
31232647	0	53	theme	multiphasic	32:42	arg1	degeneration					44:55	the multiphasic degeneration	28:55	the multiphasic degeneration of the bone-cartilage unit	28:82	Computational simulation of the multiphasic degeneration of the bone-cartilage unit during osteoarthritis via indentation and unconfined compression tests.					
31232647	3	54	from	variations	733:742	arg1	parameters					760:769	the dominant parameters	747:769	the dominant parameters	747:769	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	4	55	theme	fraction	979:986	arg1	increase					988:995	the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase	897:995	the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis	897:1041	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	3	56	theme	swelling	677:684	arg1	models					686:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	3	57	theme	validated	615:623	arg1	models					686:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	6	58	theme	significant	1438:1448	arg1	role					1464:1467	the significant biomechanical role	1434:1467	the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration	1434:1730	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	3	59	theme	unconfined	825:834	arg1	tests					848:852	unconfined compression tests	825:852	unconfined compression tests	825:852	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	4	60	theme	fluid	973:977	arg1	fraction					979:986	fluid fraction	973:986	fluid fraction	973:986	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	4	61	theme	proteoglycan	901:912	arg1	depletion					914:922	proteoglycan depletion	901:922	proteoglycan depletion	901:922	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	6	62	theme	loads	1707:1711	arg1	%					1698:1698	70%	1696:1698	70% of the loads after degeneration	1696:1730	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	6	62	theme	loads	1707:1711	arg1	loads					1707:1711	the loads	1703:1711	the loads after degeneration	1703:1730	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	3	63	theme	compression	836:846	arg1	tests					848:852	unconfined compression tests	825:852	unconfined compression tests	825:852	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	4	64	theme	mentioned	859:867	arg1	parameters					878:887	The mentioned dominant parameters	855:887	The mentioned dominant parameters	855:887	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	3	65	theme	fibril-reinforced	654:670	arg1	models					686:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	6	66	theme	constituents	1618:1629	arg1	contributions					1581:1593	relative load contributions	1567:1593	relative load contributions of articular cartilage constituents	1567:1629	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	6	67	theme	cartilage	1608:1616	arg1	constituents					1618:1629	articular cartilage constituents	1598:1629	articular cartilage constituents	1598:1629	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	0	68	theme	unit	79:82	arg1	degeneration					44:55	the multiphasic degeneration	28:55	the multiphasic degeneration of the bone-cartilage unit	28:82	Computational simulation of the multiphasic degeneration of the bone-cartilage unit during osteoarthritis via indentation and unconfined compression tests.					
31232647	5	69	theme	fluid	1178:1182	arg1	permeation					1184:1193	the fluid permeation and pressure	1174:1206	permeation	1184:1193	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	3	70	theme	depth-dependent	637:651	arg1	models					686:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models	607:691	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	7	71	theme	simulation	1845:1854	arg1	significance					1798:1809	the significance	1794:1809	the significance of the multiphasic osteoarthritis simulation	1794:1854	To conclude, the new insights provided by the results reveal the significance of the multiphasic osteoarthritis simulation and demonstrate the functionality of the proposed bone-cartilage unit model.					
31232647	3	72	theme	finite	546:551	arg1	model					561:565	a new finite element model	540:565	a new finite element model of the bone-cartilage unit	540:592	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	6	73	theme	articular	1598:1606	arg1	constituents					1618:1629	articular cartilage constituents	1598:1629	articular cartilage constituents	1598:1629	Furthermore, the osteoarthritis composition-based studies shed light on the significant biomechanical role of the calcified cartilage, which experienced a maximum change of 70 kPa in stress, together with relative load contributions of articular cartilage constituents during osteoarthritis, in which the osmotic pressure bore around 70% of the loads after degeneration.					
31232647	1	74	theme	different	253:261	arg1	tissues					280:286	different subchondral bone tissues	253:286	different subchondral bone tissues	253:286	It has been experimentally proposed that the discrete regions of articular cartilage, along with different subchondral bone tissues, known as the bone-cartilage unit, are biomechanically altered during osteoarthritis degeneration.					
31232647	0	75	theme	bone-cartilage	64:77	arg1	unit					79:82	the bone-cartilage unit	60:82	the bone-cartilage unit	60:82	Computational simulation of the multiphasic degeneration of the bone-cartilage unit during osteoarthritis via indentation and unconfined compression tests.					
31232647	4	76	theme	dominant	869:876	arg1	parameters					878:887	The mentioned dominant parameters	855:887	The mentioned dominant parameters	855:887	The mentioned dominant parameters include the proteoglycan depletion, collagen fibrillar softening, permeability, and fluid fraction increase for approximately non-advanced osteoarthritis.					
31232647	3	77	theme	element	553:559	arg1	model					561:565	a new finite element model	540:565	a new finite element model of the bone-cartilage unit	540:592	This article proposes a new finite element model of the bone-cartilage unit by combining several validated, nonlinear, depth-dependent, fibril-reinforced, and swelling models, which can computationally simulate the variations in the dominant parameters during osteoarthritis degeneration by indentation and unconfined compression tests.					
31232647	5	78	theme	osteoarthritis	1346:1359	arg1	pathogenesis					1330:1341	the pathogenesis	1326:1341	the pathogenesis of osteoarthritis	1326:1359	The results depict the importance of subchondral bone tissues in fluid distribution within the bone-cartilage units by decreasing the fluid permeation and pressure (up to a maximum of 100 kPa) during osteoarthritis, supporting the notion that subchondral bones might play a role in the pathogenesis of osteoarthritis.					
31232647	1	79	theme	subchondral	263:273	arg1	tissues					280:286	different subchondral bone tissues	253:286	different subchondral bone tissues	253:286	It has been experimentally proposed that the discrete regions of articular cartilage, along with different subchondral bone tissues, known as the bone-cartilage unit, are biomechanically altered during osteoarthritis degeneration.					
29524485	1	0	theme	chitosan/graphene	150:166	arg1	MCGON					189:193	MCGON	189:193	MCGON	189:193	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	1	0	theme	chitosan/graphene	150:166	arg1	nanocomposite					174:186	a novel magnetic chitosan/graphene oxide nanocomposite	133:186	a novel magnetic chitosan/graphene oxide nanocomposite (MCGON)	133:194	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	1	1	theme	aqueous	241:247	arg1	solutions					249:257	aqueous solutions	241:257	aqueous solutions	241:257	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	0	2	theme	chitosan/graphene	72:88	arg1	oxide					90:94	modified magnetic chitosan/graphene oxide	54:94	modified magnetic chitosan/graphene oxide	54:94	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide nanocomposites.					
29524485	1	3	theme	oxide	168:172	arg1	MCGON					189:193	MCGON	189:193	MCGON	189:193	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	1	3	theme	oxide	168:172	arg1	nanocomposite					174:186	a novel magnetic chitosan/graphene oxide nanocomposite	133:186	a novel magnetic chitosan/graphene oxide nanocomposite (MCGON)	133:194	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	6	4	theme	copper	1009:1014	arg1	ions					1021:1024	copper (II) ions	1009:1024	copper (II) ions	1009:1024	Kinetics, thermodynamics and equilibrium isotherm of the removal of copper (II) ions were carefully investigated.					
29524485	4	5	theme	strong	702:707	arg1	magnetization					720:732	strong saturation magnetization	702:732	strong saturation magnetization (3.82emug-1)	702:745	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	5	theme	strong	702:707	arg1	3.82emug-1					735:744	3.82emug-1	735:744	3.82emug-1	735:744	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	5	6	theme	Cu2+	870:873	arg1	adsorption					856:865	the adsorption	852:865	the adsorption of Cu2+ onto MCGON	852:884	The results showed that the adsorption of Cu2+ onto MCGON exhibited a maximum adsorption capacity of 217.4mgg-1.					
29524485	4	7	theme	particle	677:684	arg1	15nm					692:695	15nm	692:695	15nm	692:695	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	7	theme	particle	677:684	arg1	size					686:689	small particle size	671:689	small particle size (15nm)	671:696	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	7	8	theme	isotherm	1135:1142	arg1	models					1144:1149	Langmuir isotherm models	1126:1149	Langmuir isotherm models	1126:1149	Adsorption of Cu2+ onto nanocomposite followed pseudo-second-order and Langmuir isotherm models.					
29524485	4	9	theme	pore	645:648	arg1	4.03cm3g-1					658:667	4.03cm3g-1	658:667	4.03cm3g-1	658:667	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	9	theme	pore	645:648	arg1	volume					650:655	large pore volume	639:655	large pore volume (4.03cm3g-1)	639:668	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	10	theme	large	639:643	arg1	4.03cm3g-1					658:667	4.03cm3g-1	658:667	4.03cm3g-1	658:667	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	10	theme	large	639:643	arg1	volume					650:655	large pore volume	639:655	large pore volume (4.03cm3g-1)	639:668	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	1	11	theme	present	119:125	arg1	work					127:130	the present work	115:130	the present work	115:130	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	4	12	used	used	751:754	arg2	MCGON					582:586	The magnetic MCGON	569:586	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1)	569:745	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	12	used	used	751:754	arg2	adsorbent					772:780	adsorbent	772:780	adsorbent	772:780	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	2	13	theme	chitosan-g-poly	368:382	arg1	copolymer					444:452	chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer	368:452	chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer	368:452	The adsorbent was composed of graphene oxide (GO) modified by ethylenediamine (ED), Fe3O4 nanoparticles and chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer.					
29524485	6	14	dep	Kinetics	941:948	arg1	isotherm					982:989	isotherm	982:989	isotherm	982:989	Kinetics, thermodynamics and equilibrium isotherm of the removal of copper (II) ions were carefully investigated.					
29524485	5	15	theme	maximum	898:904	arg1	capacity					917:924	a maximum adsorption capacity	896:924	a maximum adsorption capacity of 217.4mgg-1	896:938	The results showed that the adsorption of Cu2+ onto MCGON exhibited a maximum adsorption capacity of 217.4mgg-1.					
29524485	4	16	theme	small	671:675	arg1	15nm					692:695	15nm	692:695	15nm	692:695	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	16	theme	small	671:675	arg1	size					686:689	small particle size	671:689	small particle size (15nm)	671:696	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	0	17	from	solutions	41:49	arg1	removal					10:16	Effective removal	0:16	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide	0:94	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide nanocomposites.					
29524485	8	18	theme	sorption	1196:1203	arg1	feasible					1217:1224	feasible	1217:1224	feasible	1217:1224	Thermodynamic parameters exhibited that the sorption process was feasible, spontaneous and endothermic in nature.					
29524485	8	18	theme	sorption	1196:1203	arg1	process					1205:1211	the sorption process	1192:1211	the sorption process	1192:1211	Thermodynamic parameters exhibited that the sorption process was feasible, spontaneous and endothermic in nature.					
29524485	0	19	theme	Effective	0:8	arg1	removal					10:16	Effective removal	0:16	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide	0:94	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide nanocomposites.					
29524485	2	20	theme	Fe3O4	344:348	arg1	nanoparticles					350:362	Fe3O4 nanoparticles	344:362	Fe3O4 nanoparticles	344:362	The adsorbent was composed of graphene oxide (GO) modified by ethylenediamine (ED), Fe3O4 nanoparticles and chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer.					
29524485	0	21	theme	copper	21:26	arg1	removal					10:16	Effective removal	0:16	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide	0:94	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide nanocomposites.					
29524485	5	22	theme	adsorption	906:915	arg1	capacity					917:924	a maximum adsorption capacity	896:924	a maximum adsorption capacity of 217.4mgg-1	896:938	The results showed that the adsorption of Cu2+ onto MCGON exhibited a maximum adsorption capacity of 217.4mgg-1.					
29524485	8	23	from	nature	1258:1263	arg1	feasible					1217:1224	feasible	1217:1224	feasible	1217:1224	Thermodynamic parameters exhibited that the sorption process was feasible, spontaneous and endothermic in nature.					
29524485	8	23	from	nature	1258:1263	arg1	process					1205:1211	the sorption process	1192:1211	the sorption process	1192:1211	Thermodynamic parameters exhibited that the sorption process was feasible, spontaneous and endothermic in nature.					
29524485	9	24	from	wastewater	1356:1365	arg1	removal					1316:1322	the efficient removal	1302:1322	the efficient removal of heavy metals from industrial wastewater	1302:1365	These results provide evidences for the efficient removal of heavy metals from industrial wastewater.					
29524485	9	25	theme	heavy	1327:1331	arg1	metals					1333:1338	heavy metals	1327:1338	heavy metals	1327:1338	These results provide evidences for the efficient removal of heavy metals from industrial wastewater.					
29524485	2	26	theme	sulfonic	429:436	arg1	acid					438:441	acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid	384:441	acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid	384:441	The adsorbent was composed of graphene oxide (GO) modified by ethylenediamine (ED), Fe3O4 nanoparticles and chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer.					
29524485	8	27	from	feasible	1217:1224	arg1	nature					1258:1263	nature	1258:1263	nature	1258:1263	Thermodynamic parameters exhibited that the sorption process was feasible, spontaneous and endothermic in nature.					
29524485	2	28	theme	graphene	290:297	arg1	oxide					299:303	graphene oxide	290:303	graphene oxide (GO) modified by ethylenediamine (ED), Fe3O4 nanoparticles and chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer	290:452	The adsorbent was composed of graphene oxide (GO) modified by ethylenediamine (ED), Fe3O4 nanoparticles and chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer.					
29524485	2	29	theme	acid-co-2-acrylamido-2-methylpropane	392:427	arg1	acid					438:441	acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid	384:441	acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid	384:441	The adsorbent was composed of graphene oxide (GO) modified by ethylenediamine (ED), Fe3O4 nanoparticles and chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer.					
29524485	0	30	theme	aqueous	33:39	arg1	solutions					41:49	aqueous solutions	33:49	aqueous solutions	33:49	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide nanocomposites.					
29524485	4	31	theme	surface	609:615	arg1	area					617:620	a high specific surface area	593:620	a high specific surface area of (132.9m2g-1)	593:636	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	31	theme	surface	609:615	arg1	132.9m2g-1					626:635	132.9m2g-1	626:635	132.9m2g-1	626:635	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	2	32	theme	acrylic	384:390	arg1	acid					438:441	acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid	384:441	acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid	384:441	The adsorbent was composed of graphene oxide (GO) modified by ethylenediamine (ED), Fe3O4 nanoparticles and chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer.					
29524485	6	33	theme	removal	998:1004	arg1	equilibrium					970:980	equilibrium	970:980	equilibrium	970:980	Kinetics, thermodynamics and equilibrium isotherm of the removal of copper (II) ions were carefully investigated.					
29524485	6	33	theme	removal	998:1004	arg1	thermodynamics					951:964	thermodynamics	951:964	thermodynamics	951:964	Kinetics, thermodynamics and equilibrium isotherm of the removal of copper (II) ions were carefully investigated.					
29524485	6	33	theme	removal	998:1004	arg1	Kinetics					941:948	Kinetics	941:948	Kinetics	941:948	Kinetics, thermodynamics and equilibrium isotherm of the removal of copper (II) ions were carefully investigated.					
29524485	9	34	theme	efficient	1306:1314	arg1	removal					1316:1322	the efficient removal	1302:1322	the efficient removal of heavy metals from industrial wastewater	1302:1365	These results provide evidences for the efficient removal of heavy metals from industrial wastewater.					
29524485	1	35	from	solutions	249:257	arg1	removal					220:226	the removal	216:226	the removal of Cu2+ from aqueous solutions	216:257	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	4	36	with	MCGON	582:586	arg1	magnetization					720:732	strong saturation magnetization	702:732	strong saturation magnetization (3.82emug-1)	702:745	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	36	with	MCGON	582:586	arg1	4.03cm3g-1					658:667	4.03cm3g-1	658:667	4.03cm3g-1	658:667	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	36	with	MCGON	582:586	arg1	15nm					692:695	15nm	692:695	15nm	692:695	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	36	with	MCGON	582:586	arg1	3.82emug-1					735:744	3.82emug-1	735:744	3.82emug-1	735:744	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	36	with	MCGON	582:586	arg1	area					617:620	a high specific surface area	593:620	a high specific surface area of (132.9m2g-1)	593:636	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	36	with	MCGON	582:586	arg1	size					686:689	small particle size	671:689	small particle size (15nm)	671:696	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	36	with	MCGON	582:586	arg1	volume					650:655	large pore volume	639:655	large pore volume (4.03cm3g-1)	639:668	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	36	with	MCGON	582:586	arg1	132.9m2g-1					626:635	132.9m2g-1	626:635	132.9m2g-1	626:635	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	37	theme	specific	600:607	arg1	area					617:620	a high specific surface area	593:620	a high specific surface area of (132.9m2g-1)	593:636	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	37	theme	specific	600:607	arg1	132.9m2g-1					626:635	132.9m2g-1	626:635	132.9m2g-1	626:635	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	7	38	theme	Langmuir	1126:1133	arg1	models					1144:1149	Langmuir isotherm models	1126:1149	Langmuir isotherm models	1126:1149	Adsorption of Cu2+ onto nanocomposite followed pseudo-second-order and Langmuir isotherm models.					
29524485	4	39	theme	ions	806:809	arg1	removal					790:796	the removal	786:796	the removal of Cu2+ ions from wastewater	786:825	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	2	40	dep	oxide	299:303	arg1	GO					306:307	GO	306:307	GO	306:307	The adsorbent was composed of graphene oxide (GO) modified by ethylenediamine (ED), Fe3O4 nanoparticles and chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer.					
29524485	8	41	theme	Thermodynamic	1152:1164	arg1	parameters					1166:1175	Thermodynamic parameters	1152:1175	Thermodynamic parameters	1152:1175	Thermodynamic parameters exhibited that the sorption process was feasible, spontaneous and endothermic in nature.					
29524485	4	42	theme	Cu2+	801:804	arg1	ions					806:809	Cu2+ ions	801:809	Cu2+ ions	801:809	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	43	from	wastewater	816:825	arg1	removal					790:796	the removal	786:796	the removal of Cu2+ ions from wastewater	786:825	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	3	44	theme	BET	555:557	arg1	analysis					559:566	BET analysis	555:566	BET analysis	555:566	The composition and structure of adsorbents were characterized by FTIR, SEM, EDX, XRD, TGA, VSM and BET analysis.					
29524485	7	45	theme	Cu2+	1069:1072	arg1	Adsorption					1055:1064	Adsorption	1055:1064	Adsorption of Cu2+ onto nanocomposite	1055:1091	Adsorption of Cu2+ onto nanocomposite followed pseudo-second-order and Langmuir isotherm models.					
29524485	4	46	theme	saturation	709:718	arg1	magnetization					720:732	strong saturation magnetization	702:732	strong saturation magnetization (3.82emug-1)	702:745	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	46	theme	saturation	709:718	arg1	3.82emug-1					735:744	3.82emug-1	735:744	3.82emug-1	735:744	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	5	47	theme	217.4mgg-1	929:938	arg1	capacity					917:924	a maximum adsorption capacity	896:924	a maximum adsorption capacity of 217.4mgg-1	896:938	The results showed that the adsorption of Cu2+ onto MCGON exhibited a maximum adsorption capacity of 217.4mgg-1.					
29524485	6	48	theme	ions	1021:1024	arg1	removal					998:1004	the removal	994:1004	the removal of copper (II) ions	994:1024	Kinetics, thermodynamics and equilibrium isotherm of the removal of copper (II) ions were carefully investigated.					
29524485	9	49	theme	metals	1333:1338	arg1	removal					1316:1322	the efficient removal	1302:1322	the efficient removal of heavy metals from industrial wastewater	1302:1365	These results provide evidences for the efficient removal of heavy metals from industrial wastewater.					
29524485	4	50	theme	magnetic	573:580	arg1	adsorbent					772:780	adsorbent	772:780	adsorbent	772:780	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	50	theme	magnetic	573:580	arg1	MCGON					582:586	The magnetic MCGON	569:586	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1)	569:745	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	3	51	dep	composition	459:469	arg1	The					455:457	The	455:457	The	455:457	The composition and structure of adsorbents were characterized by FTIR, SEM, EDX, XRD, TGA, VSM and BET analysis.					
29524485	9	52	theme	industrial	1345:1354	arg1	wastewater					1356:1365	industrial wastewater	1345:1365	industrial wastewater	1345:1365	These results provide evidences for the efficient removal of heavy metals from industrial wastewater.					
29524485	3	53	theme	adsorbents	488:497	arg1	composition					459:469	composition	459:469	composition	459:469	The composition and structure of adsorbents were characterized by FTIR, SEM, EDX, XRD, TGA, VSM and BET analysis.					
29524485	3	53	theme	adsorbents	488:497	arg1	structure					475:483	structure	475:483	structure	475:483	The composition and structure of adsorbents were characterized by FTIR, SEM, EDX, XRD, TGA, VSM and BET analysis.					
29524485	0	54	theme	magnetic	63:70	arg1	oxide					90:94	modified magnetic chitosan/graphene oxide	54:94	modified magnetic chitosan/graphene oxide	54:94	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide nanocomposites.					
29524485	2	55	dep	chitosan-g-poly	368:382	arg1	acid					438:441	acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid	384:441	acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid	384:441	The adsorbent was composed of graphene oxide (GO) modified by ethylenediamine (ED), Fe3O4 nanoparticles and chitosan-g-poly(acrylic acid-co-2-acrylamido-2-methylpropane sulfonic acid) copolymer.					
29524485	4	56	theme	high	595:598	arg1	area					617:620	a high specific surface area	593:620	a high specific surface area of (132.9m2g-1)	593:636	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	4	56	theme	high	595:598	arg1	132.9m2g-1					626:635	132.9m2g-1	626:635	132.9m2g-1	626:635	The magnetic MCGON with a high specific surface area of (132.9m2g-1), large pore volume (4.03cm3g-1), small particle size (15nm) and strong saturation magnetization (3.82emug-1) was used as an efficient adsorbent for the removal of Cu2+ ions from wastewater.					
29524485	1	57	theme	novel	135:139	arg1	MCGON					189:193	MCGON	189:193	MCGON	189:193	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	1	57	theme	novel	135:139	arg1	nanocomposite					174:186	a novel magnetic chitosan/graphene oxide nanocomposite	133:186	a novel magnetic chitosan/graphene oxide nanocomposite (MCGON)	133:194	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	1	58	theme	Cu2+	231:234	arg1	removal					220:226	the removal	216:226	the removal of Cu2+ from aqueous solutions	216:257	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	0	59	theme	modified	54:61	arg1	oxide					90:94	modified magnetic chitosan/graphene oxide	54:94	modified magnetic chitosan/graphene oxide	54:94	Effective removal of copper from aqueous solutions by modified magnetic chitosan/graphene oxide nanocomposites.					
29524485	1	60	theme	magnetic	141:148	arg1	MCGON					189:193	MCGON	189:193	MCGON	189:193	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
29524485	1	60	theme	magnetic	141:148	arg1	nanocomposite					174:186	a novel magnetic chitosan/graphene oxide nanocomposite	133:186	a novel magnetic chitosan/graphene oxide nanocomposite (MCGON)	133:194	In the present work, a novel magnetic chitosan/graphene oxide nanocomposite (MCGON) was synthesized for the removal of Cu2+ from aqueous solutions.					
30179189	7	0	theme	selected	1181:1188	arg1	energies					1190:1197	selected energies	1181:1197	selected energies	1181:1197	These in turn can be obtained from published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies.					
30179189	14	1	theme	various	2336:2342	arg1	thicknesses					2344:2354	various thicknesses	2336:2354	various thicknesses	2336:2354	The results provide guidance for the most appropriate conditions for X-ray imaging of individual cellular components within cells of various thicknesses.					
30179189	7	2	from	energies	1190:1197	arg1	microscopy					1149:1158	scanning electron microscopy	1131:1158	scanning electron microscopy	1131:1158	These in turn can be obtained from published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies.					
30179189	7	2	from	energies	1190:1197	arg1	imaging					1170:1176	X-ray imaging	1164:1176	X-ray imaging	1164:1176	These in turn can be obtained from published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies.					
30179189	7	2	from	energies	1190:1197	arg1	analysis					1121:1128	chemical analysis	1112:1128	chemical analysis	1112:1128	These in turn can be obtained from published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies.					
30179189	11	3	theme	cellular	1818:1825	arg1	components					1827:1836	some cellular components	1813:1836	some cellular components	1813:1836	The calculations reveal why some cellular components are more visible by absorption contrast and why much greater exposure times are required to see some cellular components.					
30179189	14	4	theme	components	2309:2318	arg1	imaging					2278:2284	X-ray imaging	2272:2284	X-ray imaging of individual cellular components within cells of various thicknesses	2272:2354	The results provide guidance for the most appropriate conditions for X-ray imaging of individual cellular components within cells of various thicknesses.					
30179189	9	5	theme	refractive	1382:1391	arg1	index					1393:1397	The refractive index	1378:1397	The refractive index	1378:1397	The refractive index calculations enable the required doses and fluences to be obtained to provide images with sufficient statistical significance, for X-ray energies between 200 and 4000 eV.					
30179189	13	6	theme	similar	2154:2160	arg1	density					2146:2152	a mass density	2139:2152	a mass density similar to the surrounding cytosol or nucleosol	2139:2200	This particularly applies to those components with a high carbon content but with a mass density similar to the surrounding cytosol or nucleosol.					
30179189	14	7	theme	individual	2289:2298	arg1	components					2309:2318	individual cellular components	2289:2318	individual cellular components within cells of various thicknesses	2289:2354	The results provide guidance for the most appropriate conditions for X-ray imaging of individual cellular components within cells of various thicknesses.					
30179189	5	8	theme	components	818:827	arg1	cytosol					849:855	the surrounding cytosol	833:855	the surrounding cytosol	833:855	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	5	8	theme	components	818:827	arg1	fluids					875:880	the fluids	871:880	the fluids present in the cellular cytoplasm and nucleus	871:926	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	5	8	theme	components	818:827	arg1	indices					794:800	the complex refractive indices	771:800	the complex refractive indices of the cellular components	771:827	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	5	8	theme	components	818:827	arg1	nucleosol					860:868	nucleosol	860:868	nucleosol	860:868	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	12	9	theme	water	1933:1937	arg1	window					1939:1944	the water window	1929:1944	the water window	1929:1944	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	12	10	theme	contrast	2034:2041	arg1	measurements					2043:2054	the phase contrast measurements	2024:2054	the phase contrast measurements	2024:2054	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	8	11	theme	lipid	1307:1311	arg1	droplets					1313:1320	lipid droplets	1307:1320	lipid droplets	1307:1320	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	5	12	theme	complex	775:781	arg1	indices					794:800	the complex refractive indices	771:800	the complex refractive indices of the cellular components	771:827	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	12	13	theme	energy	1894:1899	arg1	function					1875:1882	a function	1873:1882	a function of photon energy with absorption contrast in the water window	1873:1944	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	8	14	theme	refractive	1217:1226	arg1	indices					1228:1234	the refractive indices	1213:1234	the refractive indices of heterochromatin	1213:1253	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	8	14	theme	refractive	1217:1226	arg1	examples					1203:1210	examples	1203:1210	examples	1203:1210	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	10	15	theme	statistical	1574:1584	arg1	significance					1586:1597	The statistical significance	1570:1597	The statistical significance (e.g. the Rose criterion) for various requirements	1570:1648	The statistical significance (e.g. the Rose criterion) for various requirements is discussed.					
30179189	12	16	theme	absorption	1906:1915	arg1	contrast					1917:1924	absorption contrast	1906:1924	absorption contrast	1906:1924	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	11	17	theme	greater	1770:1776	arg1	times					1787:1791	much greater exposure times	1765:1791	much greater exposure times	1765:1791	The calculations reveal why some cellular components are more visible by absorption contrast and why much greater exposure times are required to see some cellular components.					
30179189	1	18	theme	biological	106:115	arg1	cells					117:121	biological cells	106:121	biological cells	106:121	X-ray imaging allows biological cells to be examined at a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples.					
30179189	4	19	theme	X-ray	660:664	arg1	imaging					679:685	X-ray fluorescence imaging	660:685	X-ray fluorescence imaging	660:685	In general these operate at a higher energy, enabling thicker cells to be examined and, in some cases, can be combined with X-ray fluorescence imaging to locate specific metals.					
30179189	9	20	theme	X-ray	1530:1534	arg1	energies					1536:1543	X-ray energies	1530:1543	X-ray energies between 200 and 4000 eV	1530:1567	The refractive index calculations enable the required doses and fluences to be obtained to provide images with sufficient statistical significance, for X-ray energies between 200 and 4000 eV.					
30179189	12	21	from	function	1875:1882	arg1	window					1939:1944	the water window	1929:1944	the water window	1929:1944	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	12	22	theme	higher	1984:1989	arg1	doses					1991:1995	much higher doses	1979:1995	much higher doses	1979:1995	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	14	23	theme	X-ray	2272:2276	arg1	imaging					2278:2284	X-ray imaging	2272:2284	X-ray imaging of individual cellular components within cells of various thicknesses	2272:2354	The results provide guidance for the most appropriate conditions for X-ray imaging of individual cellular components within cells of various thicknesses.					
30179189	0	24	theme	X-ray	50:54	arg1	imaging					56:62	X-ray imaging	50:62	X-ray imaging of biological cells	50:82	A comparison of absorption and phase contrast for X-ray imaging of biological cells.					
30179189	7	25	theme	X-ray	1164:1168	arg1	imaging					1170:1176	X-ray imaging	1164:1176	X-ray imaging	1164:1176	These in turn can be obtained from published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies.					
30179189	6	26	theme	refractive	933:942	arg1	indices					944:950	The refractive indices	929:950	The refractive indices	929:950	The refractive indices can be calculated from the atomic composition and density of the components.					
30179189	8	27	theme	neutral	1291:1297	arg1	core					1299:1302	the neutral core	1287:1302	the neutral core of lipid droplets	1287:1320	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	8	27	theme	neutral	1291:1297	arg1	examples					1203:1210	examples	1203:1210	examples	1203:1210	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	0	28	theme	cells	78:82	arg1	imaging					56:62	X-ray imaging	50:62	X-ray imaging of biological cells	50:82	A comparison of absorption and phase contrast for X-ray imaging of biological cells.					
30179189	9	29	theme	sufficient	1489:1498	arg1	significance					1512:1523	sufficient statistical significance	1489:1523	sufficient statistical significance	1489:1523	The refractive index calculations enable the required doses and fluences to be obtained to provide images with sufficient statistical significance, for X-ray energies between 200 and 4000 eV.					
30179189	10	30	theme	Rose	1609:1612	arg1	criterion					1614:1622	the Rose criterion	1605:1622	the Rose criterion	1605:1622	The statistical significance (e.g. the Rose criterion) for various requirements is discussed.					
30179189	7	31	theme	scanning	1131:1138	arg1	microscopy					1149:1158	scanning electron microscopy	1131:1158	scanning electron microscopy	1131:1158	These in turn can be obtained from published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies.					
30179189	12	32	with	energy	1894:1899	arg1	contrast					1917:1924	absorption contrast	1906:1924	absorption contrast	1906:1924	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	0	33	theme	phase	31:35	arg1	contrast					37:44	phase contrast	31:44	phase contrast	31:44	A comparison of absorption and phase contrast for X-ray imaging of biological cells.					
30179189	13	34	theme	surrounding	2169:2179	arg1	cytosol					2181:2187	the surrounding cytosol	2165:2187	the surrounding cytosol	2165:2187	This particularly applies to those components with a high carbon content but with a mass density similar to the surrounding cytosol or nucleosol.					
30179189	8	35	theme	droplets	1313:1320	arg1	core					1299:1302	the neutral core	1287:1302	the neutral core of lipid droplets	1287:1320	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	8	35	theme	droplets	1313:1320	arg1	examples					1203:1210	examples	1203:1210	examples	1203:1210	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	1	36	theme	electron	259:266	arg1	microscopy					268:277	electron microscopy	259:277	electron microscopy of thick samples	259:294	X-ray imaging allows biological cells to be examined at a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples.					
30179189	12	37	theme	phase	1855:1859	arg1	contrast					1861:1868	phase contrast	1855:1868	phase contrast	1855:1868	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	5	38	theme	present	882:888	arg1	fluids					875:880	the fluids	871:880	the fluids present in the cellular cytoplasm and nucleus	871:926	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	5	38	theme	present	882:888	arg1	cytosol					849:855	the surrounding cytosol	833:855	the surrounding cytosol	833:855	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	8	39	theme	starch	1323:1328	arg1	granules					1330:1337	starch granules	1323:1337	starch granules	1323:1337	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	8	39	theme	starch	1323:1328	arg1	examples					1203:1210	examples	1203:1210	examples	1203:1210	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	10	40	theme	various	1629:1635	arg1	requirements					1637:1648	various requirements	1629:1648	various requirements	1629:1648	The statistical significance (e.g. the Rose criterion) for various requirements is discussed.					
30179189	8	41	theme	mitochondrial	1262:1274	arg1	membranes					1276:1284	inner mitochondrial membranes	1256:1284	inner mitochondrial membranes	1256:1284	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	8	41	theme	mitochondrial	1262:1274	arg1	examples					1203:1210	examples	1203:1210	examples	1203:1210	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	1	42	theme	samples	288:294	arg1	microscopy					268:277	electron microscopy	259:277	electron microscopy of thick samples	259:294	X-ray imaging allows biological cells to be examined at a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples.					
30179189	0	43	theme	contrast	37:44	arg1	comparison					2:11	A comparison	0:11	A comparison of absorption and phase contrast for X-ray imaging of biological cells	0:82	A comparison of absorption and phase contrast for X-ray imaging of biological cells.					
30179189	13	44	theme	mass	2141:2144	arg1	density					2146:2152	a mass density	2139:2152	a mass density similar to the surrounding cytosol or nucleosol	2139:2200	This particularly applies to those components with a high carbon content but with a mass density similar to the surrounding cytosol or nucleosol.					
30179189	13	45	theme	high	2110:2113	arg1	content					2122:2128	a high carbon content	2108:2128	a high carbon content	2108:2128	This particularly applies to those components with a high carbon content but with a mass density similar to the surrounding cytosol or nucleosol.					
30179189	1	46	with	resolution	150:159	arg1	light					188:192	visible light	180:192	visible light	180:192	X-ray imaging allows biological cells to be examined at a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples.					
30179189	4	47	theme	higher	566:571	arg1	energy					573:578	a higher energy	564:578	a higher energy	564:578	In general these operate at a higher energy, enabling thicker cells to be examined and, in some cases, can be combined with X-ray fluorescence imaging to locate specific metals.					
30179189	3	48	theme	phase	484:488	arg1	techniques					499:508	phase contrast techniques	484:508	phase contrast techniques	484:508	A variety of phase contrast techniques are also being developed.					
30179189	5	49	from	present	882:888	arg1	cytoplasm					906:914	cellular cytoplasm	897:914	cellular cytoplasm	897:914	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	5	49	from	present	882:888	arg1	nucleus					920:926	nucleus	920:926	nucleus	920:926	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	4	50	dep	examined	610:617	arg1	combined					646:653	combined	646:653	can be combined with X-ray fluorescence imaging to locate specific metals	639:711	In general these operate at a higher energy, enabling thicker cells to be examined and, in some cases, can be combined with X-ray fluorescence imaging to locate specific metals.					
30179189	14	51	theme	thicknesses	2344:2354	arg1	cells					2327:2331	cells	2327:2331	cells of various thicknesses	2327:2354	The results provide guidance for the most appropriate conditions for X-ray imaging of individual cellular components within cells of various thicknesses.					
30179189	2	52	theme	absorption	338:347	arg1	technique					325:333	The most used and developed technique	297:333	The most used and developed technique	297:333	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	2	52	theme	absorption	338:347	arg1	imaging					358:364	absorption contrast imaging	338:364	absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV	338:468	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	5	53	from	nucleus	920:926	arg1	present					882:888	present	882:888	present	882:888	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	1	54	theme	preparation	218:228	arg1	difficulties					230:241	the preparation difficulties	214:241	the preparation difficulties associated with electron microscopy of thick samples	214:294	X-ray imaging allows biological cells to be examined at a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples.					
30179189	1	55	theme	visible	180:186	arg1	light					188:192	visible light	180:192	visible light	180:192	X-ray imaging allows biological cells to be examined at a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples.					
30179189	5	56	from	cytoplasm	906:914	arg1	present					882:888	present	882:888	present	882:888	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	3	57	theme	techniques	499:508	arg1	techniques					499:508	phase contrast techniques	484:508	phase contrast techniques	484:508	A variety of phase contrast techniques are also being developed.					
30179189	3	57	theme	techniques	499:508	arg1	variety					473:479	A variety	471:479	A variety of phase contrast techniques	471:508	A variety of phase contrast techniques are also being developed.					
30179189	1	58	theme	higher	143:148	arg1	resolution					150:159	a higher resolution	141:159	a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples	141:294	X-ray imaging allows biological cells to be examined at a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples.					
30179189	14	59	theme	cellular	2300:2307	arg1	components					2309:2318	individual cellular components	2289:2318	individual cellular components within cells of various thicknesses	2289:2354	The results provide guidance for the most appropriate conditions for X-ray imaging of individual cellular components within cells of various thicknesses.					
30179189	5	60	theme	cellular	809:816	arg1	components					818:827	the cellular components	805:827	the cellular components	805:827	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	2	61	from	imaging	358:364	arg1	window					379:384	the water window	369:384	the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV	369:468	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	12	62	theme	phase	2028:2032	arg1	measurements					2043:2054	the phase contrast measurements	2024:2054	the phase contrast measurements	2024:2054	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	5	63	theme	refractive	783:792	arg1	indices					794:800	the complex refractive indices	771:800	the complex refractive indices of the cellular components	771:827	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	12	64	theme	photon	1887:1892	arg1	energy					1894:1899	photon energy	1887:1899	photon energy with absorption contrast	1887:1924	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	5	65	attach	present	882:888	arg2	fluids					875:880	the fluids	871:880	the fluids present in the cellular cytoplasm and nucleus	871:926	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	5	65	attach	present	882:888	arg2	cytosol					849:855	the surrounding cytosol	833:855	the surrounding cytosol	833:855	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	5	65	attach	present	882:888	arg1	cytoplasm					906:914	cellular cytoplasm	897:914	cellular cytoplasm	897:914	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	5	65	attach	present	882:888	arg1	nucleus					920:926	nucleus	920:926	nucleus	920:926	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	11	66	theme	exposure	1778:1785	arg1	times					1787:1791	much greater exposure times	1765:1791	much greater exposure times	1765:1791	The calculations reveal why some cellular components are more visible by absorption contrast and why much greater exposure times are required to see some cellular components.					
30179189	8	67	theme	heterochromatin	1239:1253	arg1	indices					1228:1234	the refractive indices	1213:1234	the refractive indices of heterochromatin	1213:1253	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	8	67	theme	heterochromatin	1239:1253	arg1	examples					1203:1210	examples	1203:1210	examples	1203:1210	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	8	68	theme	inner	1256:1260	arg1	membranes					1276:1284	inner mitochondrial membranes	1256:1284	inner mitochondrial membranes	1256:1284	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	8	68	theme	inner	1256:1260	arg1	examples					1203:1210	examples	1203:1210	examples	1203:1210	As examples, the refractive indices of heterochromatin, inner mitochondrial membranes, the neutral core of lipid droplets, starch granules, cytosol and nucleosol are calculated.					
30179189	14	69	theme	appropriate	2245:2255	arg1	conditions					2257:2266	the most appropriate conditions	2236:2266	the most appropriate conditions for X-ray imaging of individual cellular components within cells of various thicknesses	2236:2354	The results provide guidance for the most appropriate conditions for X-ray imaging of individual cellular components within cells of various thicknesses.					
30179189	11	70	theme	cellular	1697:1704	arg1	components					1706:1715	some cellular components	1692:1715	some cellular components	1692:1715	The calculations reveal why some cellular components are more visible by absorption contrast and why much greater exposure times are required to see some cellular components.					
30179189	0	71	theme	biological	67:76	arg1	cells					78:82	biological cells	67:82	biological cells	67:82	A comparison of absorption and phase contrast for X-ray imaging of biological cells.					
30179189	5	72	theme	surrounding	837:847	arg1	fluids					875:880	the fluids	871:880	the fluids present in the cellular cytoplasm and nucleus	871:926	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	5	72	theme	surrounding	837:847	arg1	cytosol					849:855	the surrounding cytosol	833:855	the surrounding cytosol	833:855	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	9	73	with	images	1477:1482	arg1	significance					1512:1523	sufficient statistical significance	1489:1523	sufficient statistical significance	1489:1523	The refractive index calculations enable the required doses and fluences to be obtained to provide images with sufficient statistical significance, for X-ray energies between 200 and 4000 eV.					
30179189	11	74	theme	absorption	1737:1746	arg1	contrast					1748:1755	absorption contrast	1737:1755	absorption contrast	1737:1755	The calculations reveal why some cellular components are more visible by absorption contrast and why much greater exposure times are required to see some cellular components.					
30179189	7	75	theme	electron	1140:1147	arg1	microscopy					1149:1158	scanning electron microscopy	1131:1158	scanning electron microscopy	1131:1158	These in turn can be obtained from published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies.					
30179189	5	76	theme	cellular	897:904	arg1	cytoplasm					906:914	cellular cytoplasm	897:914	cellular cytoplasm	897:914	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	4	77	theme	specific	697:704	arg1	metals					706:711	specific metals	697:711	specific metals	697:711	In general these operate at a higher energy, enabling thicker cells to be examined and, in some cases, can be combined with X-ray fluorescence imaging to locate specific metals.					
30179189	4	78	theme	thicker	590:596	arg1	cells					598:602	thicker cells	590:602	thicker cells	590:602	In general these operate at a higher energy, enabling thicker cells to be examined and, in some cases, can be combined with X-ray fluorescence imaging to locate specific metals.					
30179189	9	79	theme	statistical	1500:1510	arg1	significance					1512:1523	sufficient statistical significance	1489:1523	sufficient statistical significance	1489:1523	The refractive index calculations enable the required doses and fluences to be obtained to provide images with sufficient statistical significance, for X-ray energies between 200 and 4000 eV.					
30179189	6	80	theme	components	1017:1026	arg1	composition					986:996	atomic composition	979:996	atomic composition	979:996	The refractive indices can be calculated from the atomic composition and density of the components.					
30179189	6	80	theme	components	1017:1026	arg1	density					1002:1008	density	1002:1008	density	1002:1008	The refractive indices can be calculated from the atomic composition and density of the components.					
30179189	6	81	dep	composition	986:996	arg1	the					975:977	the	975:977	the	975:977	The refractive indices can be calculated from the atomic composition and density of the components.					
30179189	2	82	theme	water	373:377	arg1	window					379:384	the water window	369:384	the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV	369:468	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	7	83	theme	chemical	1112:1119	arg1	analysis					1121:1128	chemical analysis	1112:1128	chemical analysis	1112:1128	These in turn can be obtained from published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies.					
30179189	12	84	theme	contrast	1861:1868	arg1	comparison					1841:1850	A comparison	1839:1850	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window	1839:1944	A comparison of phase contrast as a function of photon energy with absorption contrast in the water window is provided and it is shown that much higher doses are generally required for the phase contrast measurements.					
30179189	4	85	theme	fluorescence	666:677	arg1	imaging					679:685	X-ray fluorescence imaging	660:685	X-ray fluorescence imaging	660:685	In general these operate at a higher energy, enabling thicker cells to be examined and, in some cases, can be combined with X-ray fluorescence imaging to locate specific metals.					
30179189	0	86	theme	absorption	16:25	arg1	comparison					2:11	A comparison	0:11	A comparison of absorption and phase contrast for X-ray imaging of biological cells	0:82	A comparison of absorption and phase contrast for X-ray imaging of biological cells.					
30179189	1	87	theme	X-ray	85:89	arg1	imaging					91:97	X-ray imaging	85:97	X-ray imaging	85:97	X-ray imaging allows biological cells to be examined at a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples.					
30179189	5	88	theme	various	718:724	arg1	methods					726:732	The various methods	714:732	The various methods	714:732	The various methods are based on the differences between the complex refractive indices of the cellular components and the surrounding cytosol or nucleosol, the fluids present in the cellular cytoplasm and nucleus.					
30179189	1	89	theme	thick	282:286	arg1	samples					288:294	thick samples	282:294	thick samples	282:294	X-ray imaging allows biological cells to be examined at a higher resolution than possible with visible light and without some of the preparation difficulties associated with electron microscopy of thick samples.					
30179189	9	90	theme	required	1423:1430	arg1	doses					1432:1436	the required doses	1419:1436	the required doses	1419:1436	The refractive index calculations enable the required doses and fluences to be obtained to provide images with sufficient statistical significance, for X-ray energies between 200 and 4000 eV.					
30179189	6	91	theme	atomic	979:984	arg1	composition					986:996	atomic composition	979:996	atomic composition	979:996	The refractive indices can be calculated from the atomic composition and density of the components.					
30179189	10	92	dep	criterion	1614:1622	arg1	e.g.					1600:1603	e.g.	1600:1603	e.g.	1600:1603	The statistical significance (e.g. the Rose criterion) for various requirements is discussed.					
30179189	7	93	theme	published	1064:1072	arg1	measurements					1074:1085	published measurements	1064:1085	published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies	1064:1197	These in turn can be obtained from published measurements using techniques such as chemical analysis, scanning electron microscopy and X-ray imaging at selected energies.					
30179189	2	94	theme	contrast	349:356	arg1	technique					325:333	The most used and developed technique	297:333	The most used and developed technique	297:333	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	2	94	theme	contrast	349:356	arg1	imaging					358:364	absorption contrast imaging	338:364	absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV	338:468	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	2	95	theme	developed	315:323	arg1	technique					325:333	The most used and developed technique	297:333	The most used and developed technique	297:333	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	2	95	theme	developed	315:323	arg1	imaging					358:364	absorption contrast imaging	338:364	absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV	338:468	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	13	96	with	components	2092:2101	arg1	density					2146:2152	a mass density	2139:2152	a mass density similar to the surrounding cytosol or nucleosol	2139:2200	This particularly applies to those components with a high carbon content but with a mass density similar to the surrounding cytosol or nucleosol.					
30179189	13	96	with	components	2092:2101	arg1	content					2122:2128	a high carbon content	2108:2128	a high carbon content	2108:2128	This particularly applies to those components with a high carbon content but with a mass density similar to the surrounding cytosol or nucleosol.					
30179189	2	97	theme	used	306:309	arg1	technique					325:333	The most used and developed technique	297:333	The most used and developed technique	297:333	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	2	97	theme	used	306:309	arg1	imaging					358:364	absorption contrast imaging	338:364	absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV	338:468	The most used and developed technique is absorption contrast imaging in the water window which exploits the contrast between carbon and oxygen at an energy of around 500 eV.					
30179189	13	98	theme	carbon	2115:2120	arg1	content					2122:2128	a high carbon content	2108:2128	a high carbon content	2108:2128	This particularly applies to those components with a high carbon content but with a mass density similar to the surrounding cytosol or nucleosol.					
30179189	3	99	theme	contrast	490:497	arg1	techniques					499:508	phase contrast techniques	484:508	phase contrast techniques	484:508	A variety of phase contrast techniques are also being developed.					
30058853	8	0	theme	tendon-to-triceps	1163:1179	arg1	intensity					1203:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity	1149:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity ratio	1149:1217	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	11	1	theme	trilaminar	1617:1626	arg1	appearance					1628:1637	a trilaminar appearance	1615:1637	a trilaminar appearance	1615:1637	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	7	2	theme	thick	878:882	arg1	layer					905:909	a thick, hyperintense center layer	876:909	a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins	876:994	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	7	2	theme	thick	878:882	arg1	substance					920:928	central substance	912:928	central substance	912:928	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	8	3	theme	muscle	1189:1194	arg1	intensity					1203:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity	1149:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity ratio	1149:1217	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	12	4	theme	shoulder	1810:1817	arg1	disease					1825:1831	shoulder joint disease	1810:1831	shoulder joint disease	1810:1831	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	2	5	theme	intact	270:275	arg1	Beagles					282:288	nonlame 2-year-old sexually intact male Beagles	242:288	nonlame 2-year-old sexually intact male Beagles	242:288	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	8	6	theme	intensity	1203:1211	arg1	ratio					1213:1217	supraspinatus tendon-to-triceps brachii muscle signal intensity ratio	1149:1217	supraspinatus tendon-to-triceps brachii muscle signal intensity ratio	1149:1217	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	12	7	theme	disease	1825:1831	arg1	evidence					1798:1805	other MRI evidence	1788:1805	other MRI evidence of shoulder joint disease and diagnostic findings	1788:1855	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	7	8	dep	thick	878:882	arg1	hyperintense					885:896	hyperintense	885:896	hyperintense	885:896	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	2	9	theme	2-year-old	250:259	arg1	Beagles					282:288	nonlame 2-year-old sexually intact male Beagles	242:288	nonlame 2-year-old sexually intact male Beagles	242:288	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	1	10	theme	tendon	175:180	arg1	features					145:152	the MRI and histologic features	122:152	the MRI and histologic features of the supraspinatus tendon in nonlame dogs	122:196	OBJECTIVE To characterize the MRI and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	8	11	theme	±	1006:1006	arg1	margin					1044:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	1	12	theme	nonlame	185:191	arg1	dogs					193:196	nonlame dogs	185:196	nonlame dogs	185:196	OBJECTIVE To characterize the MRI and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	8	13	theme	central	1011:1017	arg1	margin					1044:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	2	14	theme	Beagles	282:288	arg1	cadavers					209:216	ANIMALS 7 cadavers	199:216	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.	199:289	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	2	14	theme	Beagles	282:288	arg1	joints					231:236	14 shoulder joints	219:236	14 shoulder joints	219:236	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	7	15	theme	MRI	837:839	arg1	images					841:846	sagittal and transverse MRI images	813:846	sagittal and transverse MRI images	813:846	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	12	16	theme	supraspinatus	1678:1690	arg1	tendinosis					1692:1701	supraspinatus tendinosis	1678:1701	supraspinatus tendinosis	1678:1701	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	8	17	theme	tendon	1238:1243	arg1	volume					1245:1250	tendon volume	1238:1250	tendon volume	1238:1250	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	1	18	from	dogs	193:196	arg1	features					145:152	the MRI and histologic features	122:152	the MRI and histologic features of the supraspinatus tendon in nonlame dogs	122:196	OBJECTIVE To characterize the MRI and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	12	19	theme	MRI	1776:1778	arg1	images					1780:1785	MRI images	1776:1785	MRI images	1776:1785	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	12	20	from	signal	1766:1771	arg1	images					1780:1785	MRI images	1776:1785	MRI images	1776:1785	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	9	21	with	tendons	1334:1340	arg1	fibers					1371:1376	highly ordered collagen fibers	1347:1376	highly ordered collagen fibers	1347:1376	The superficial and deep margins histologically resembled other tendons with highly ordered collagen fibers.					
30058853	3	22	theme	pulse	331:335	arg1	sequences					337:345	Multiple MRI fluid-sensitive pulse sequences	302:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences	291:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	0	23	theme	supraspinatus	58:70	arg1	tendon					72:77	the supraspinatus tendon	54:77	the supraspinatus tendon	54:77	Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	9	24	theme	superficial	1274:1284	arg1	margins					1295:1301	The superficial and deep margins	1270:1301	The superficial and deep margins	1270:1301	The superficial and deep margins histologically resembled other tendons with highly ordered collagen fibers.					
30058853	3	25	theme	MRI	311:313	arg1	sequences					337:345	Multiple MRI fluid-sensitive pulse sequences	302:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences	291:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	0	26	from	imaging	19:25	arg1	dogs					90:93	nonlame dogs	82:93	nonlame dogs	82:93	Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	9	27	theme	deep	1290:1293	arg1	margins					1295:1301	The superficial and deep margins	1270:1301	The superficial and deep margins	1270:1301	The superficial and deep margins histologically resembled other tendons with highly ordered collagen fibers.					
30058853	8	28	theme	margin	1044:1049	arg1	ratios					1098:1103	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios	997:1103	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios	997:1103	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	11	29	contain	have	1610:1613	arg2	appearance					1628:1637	a trilaminar appearance	1615:1637	a trilaminar appearance	1615:1637	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	11	29	contain	have	1610:1613	arg1	Results					1541:1547	CONCLUSIONS AND CLINICAL RELEVANCE Results	1506:1547	Results	1541:1547	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	10	30	theme	arranged	1479:1486	arg1	bundles					1497:1503	haphazardly arranged collagen bundles	1467:1503	haphazardly arranged collagen bundles	1467:1503	The central substance was comprised of water-rich glycosaminoglycans interspersed among haphazardly arranged collagen bundles.					
30058853	11	31	theme	canine	1581:1586	arg1	tendons					1602:1608	histologically normal canine supraspinatus tendons	1559:1608	histologically normal canine supraspinatus tendons	1559:1608	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	8	32	theme	central	1055:1061	arg1	thickness					1088:1096	central substance-to-deep margin thickness	1055:1096	central substance-to-deep margin thickness	1055:1096	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	7	33	theme	hypointense	955:965	arg1	margins					988:994	thin hypointense superficial and deep margins	950:994	thin hypointense superficial and deep margins	950:994	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	9	34	theme	other	1328:1332	arg1	tendons					1334:1340	other tendons	1328:1340	other tendons with highly ordered collagen fibers	1328:1376	The superficial and deep margins histologically resembled other tendons with highly ordered collagen fibers.					
30058853	8	35	theme	margin	1081:1086	arg1	thickness					1088:1096	central substance-to-deep margin thickness	1055:1096	central substance-to-deep margin thickness	1055:1096	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	9	36	theme	collagen	1362:1369	arg1	fibers					1371:1376	highly ordered collagen fibers	1347:1376	highly ordered collagen fibers	1347:1376	The superficial and deep margins histologically resembled other tendons with highly ordered collagen fibers.					
30058853	0	37	theme	Magnetic	0:7	arg1	imaging					19:25	Magnetic resonance imaging	0:25	Magnetic resonance imaging	0:25	Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	3	38	theme	shoulder	370:377	arg1	joints					379:384	both shoulder joints	365:384	both shoulder joints of each cadaver	365:400	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	11	39	theme	CLINICAL	1522:1529	arg1	RELEVANCE					1531:1539	CLINICAL RELEVANCE	1522:1539	CLINICAL RELEVANCE	1522:1539	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	9	40	theme	ordered	1354:1360	arg1	fibers					1371:1376	highly ordered collagen fibers	1347:1376	highly ordered collagen fibers	1347:1376	The superficial and deep margins histologically resembled other tendons with highly ordered collagen fibers.					
30058853	7	41	dep	RESULTS	748:754	arg1	had					782:784	had	782:784	had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins	782:994	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	11	42	theme	CONCLUSIONS	1506:1516	arg1	Results					1541:1547	CONCLUSIONS AND CLINICAL RELEVANCE Results	1506:1547	Results	1541:1547	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	0	43	theme	histologic	31:40	arg1	features					42:49	histologic features	31:49	histologic features	31:49	Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	7	44	theme	trilaminar	788:797	arg1	appearance					799:808	a trilaminar appearance	786:808	a trilaminar appearance	786:808	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	3	45	theme	cadaver	394:400	arg1	joints					379:384	both shoulder joints	365:384	both shoulder joints of each cadaver	365:400	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	3	46	theme	tendon	473:478	arg1	volume					422:427	volume	422:427	volume	422:427	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	3	46	theme	tendon	473:478	arg1	thickness					411:419	thickness	411:419	thickness	411:419	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	3	46	theme	tendon	473:478	arg1	intensity					441:449	signal intensity	434:449	signal intensity	434:449	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	2	47	theme	ANIMALS	199:205	arg1	cadavers					209:216	ANIMALS 7 cadavers	199:216	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.	199:289	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	2	47	theme	ANIMALS	199:205	arg1	joints					231:236	14 shoulder joints	219:236	14 shoulder joints	219:236	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	7	48	theme	supraspinatus	760:772	arg1	tendons					774:780	All supraspinatus tendons	756:780	All supraspinatus tendons	756:780	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	12	49	theme	findings	1848:1855	arg1	evidence					1798:1805	other MRI evidence	1788:1805	other MRI evidence of shoulder joint disease and diagnostic findings	1788:1855	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	7	50	theme	center	898:903	arg1	layer					905:909	a thick, hyperintense center layer	876:909	a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins	876:994	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	7	50	theme	center	898:903	arg1	substance					920:928	central substance	912:928	central substance	912:928	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	8	51	theme	supraspinatus	1149:1161	arg1	intensity					1203:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity	1149:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity ratio	1149:1217	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	12	52	theme	joint	1819:1823	arg1	disease					1825:1831	shoulder joint disease	1810:1831	shoulder joint disease	1810:1831	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	12	53	theme	other	1788:1792	arg1	evidence					1798:1805	other MRI evidence	1788:1805	other MRI evidence of shoulder joint disease and diagnostic findings	1788:1855	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	5	54	theme	tendon	654:659	arg1	morphology					661:670	tendon morphology	654:670	tendon morphology	654:670	Tissue specimens were stained with various stains to determine tendon morphology and composition.					
30058853	2	55	theme	male	277:280	arg1	Beagles					282:288	nonlame 2-year-old sexually intact male Beagles	242:288	nonlame 2-year-old sexually intact male Beagles	242:288	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	8	56	theme	brachii	1181:1187	arg1	intensity					1203:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity	1149:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity ratio	1149:1217	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	8	57	theme	mean	1001:1004	arg1	margin					1044:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	7	58	contain	had	782:784	arg2	appearance					799:808	a trilaminar appearance	786:808	a trilaminar appearance	786:808	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	7	58	contain	had	782:784	arg1	tendons					774:780	All supraspinatus tendons	756:780	All supraspinatus tendons	756:780	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	4	59	theme	histologic	567:576	arg1	examination					578:588	histologic examination	567:588	histologic examination	567:588	After MRI scanning was complete, the shoulder joints were processed for histologic examination.					
30058853	3	60	dep	PROCEDURES	291:300	arg1	sequences					337:345	Multiple MRI fluid-sensitive pulse sequences	302:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences	291:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	1	61	theme	supraspinatus	161:173	arg1	tendon					175:180	the supraspinatus tendon	157:180	the supraspinatus tendon in nonlame dogs	157:196	OBJECTIVE To characterize the MRI and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	8	62	theme	signal	1196:1201	arg1	intensity					1203:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity	1149:1211	supraspinatus tendon-to-triceps brachii muscle signal intensity ratio	1149:1217	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	8	63	theme	SD	1008:1009	arg1	margin					1044:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	5	64	theme	various	626:632	arg1	stains					634:639	various stains	626:639	various stains	626:639	Tissue specimens were stained with various stains to determine tendon morphology and composition.					
30058853	8	65	theme	substance-to-superficial	1019:1042	arg1	margin					1044:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin	997:1049	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	8	66	theme	445	1256:1258	arg1	±					1260:1260	±	1260:1260	±	1260:1260	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	10	67	theme	water-rich	1418:1427	arg1	glycosaminoglycans					1429:1446	water-rich glycosaminoglycans	1418:1446	water-rich glycosaminoglycans interspersed among haphazardly arranged collagen bundles	1418:1503	The central substance was comprised of water-rich glycosaminoglycans interspersed among haphazardly arranged collagen bundles.					
30058853	12	68	theme	tendinosis	1692:1701	arg1	diagnosis					1665:1673	a diagnosis	1663:1673	a diagnosis of supraspinatus tendinosis	1663:1701	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	7	69	theme	superficial	967:977	arg1	margins					988:994	thin hypointense superficial and deep margins	950:994	thin hypointense superficial and deep margins	950:994	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	8	70	theme	20	1262:1263	arg1	±					1260:1260	±	1260:1260	±	1260:1260	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	2	71	theme	shoulder	222:229	arg1	cadavers					209:216	ANIMALS 7 cadavers	199:216	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.	199:289	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	2	71	theme	shoulder	222:229	arg1	joints					231:236	14 shoulder joints	219:236	14 shoulder joints	219:236	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	3	72	theme	Multiple	302:309	arg1	sequences					337:345	Multiple MRI fluid-sensitive pulse sequences	302:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences	291:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	11	73	theme	MRI	1642:1644	arg1	images					1646:1651	MRI images	1642:1651	MRI images	1642:1651	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	2	74	theme	nonlame	242:248	arg1	Beagles					282:288	nonlame 2-year-old sexually intact male Beagles	242:288	nonlame 2-year-old sexually intact male Beagles	242:288	ANIMALS 7 cadavers (14 shoulder joints) of nonlame 2-year-old sexually intact male Beagles.					
30058853	7	75	theme	deep	983:986	arg1	margins					988:994	thin hypointense superficial and deep margins	950:994	thin hypointense superficial and deep margins	950:994	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	1	76	theme	MRI	126:128	arg1	features					145:152	the MRI and histologic features	122:152	the MRI and histologic features of the supraspinatus tendon in nonlame dogs	122:196	OBJECTIVE To characterize the MRI and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	12	77	theme	MRI	1794:1796	arg1	evidence					1798:1805	other MRI evidence	1788:1805	other MRI evidence of shoulder joint disease and diagnostic findings	1788:1855	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	11	78	theme	RELEVANCE	1531:1539	arg1	Results					1541:1547	CONCLUSIONS AND CLINICAL RELEVANCE Results	1506:1547	Results	1541:1547	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	0	79	theme	tendon	72:77	arg1	imaging					19:25	Magnetic resonance imaging	0:25	Magnetic resonance imaging	0:25	Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	0	79	theme	tendon	72:77	arg1	features					42:49	histologic features	31:49	histologic features	31:49	Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	1	80	theme	histologic	134:143	arg1	features					145:152	the MRI and histologic features	122:152	the MRI and histologic features of the supraspinatus tendon in nonlame dogs	122:196	OBJECTIVE To characterize the MRI and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	11	81	theme	supraspinatus	1588:1600	arg1	tendons					1602:1608	histologically normal canine supraspinatus tendons	1559:1608	histologically normal canine supraspinatus tendons	1559:1608	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	0	82	theme	nonlame	82:88	arg1	dogs					90:93	nonlame dogs	82:93	nonlame dogs	82:93	Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	1	83	from	tendon	175:180	arg1	dogs					193:196	nonlame dogs	185:196	nonlame dogs	185:196	OBJECTIVE To characterize the MRI and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	3	84	theme	fluid-sensitive	315:329	arg1	sequences					337:345	Multiple MRI fluid-sensitive pulse sequences	302:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences	291:345	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	4	85	theme	MRI	501:503	arg1	scanning					505:512	MRI scanning	501:512	MRI scanning	501:512	After MRI scanning was complete, the shoulder joints were processed for histologic examination.					
30058853	10	86	theme	collagen	1488:1495	arg1	bundles					1497:1503	haphazardly arranged collagen bundles	1467:1503	haphazardly arranged collagen bundles	1467:1503	The central substance was comprised of water-rich glycosaminoglycans interspersed among haphazardly arranged collagen bundles.					
30058853	11	87	theme	normal	1574:1579	arg1	tendons					1602:1608	histologically normal canine supraspinatus tendons	1559:1608	histologically normal canine supraspinatus tendons	1559:1608	CONCLUSIONS AND CLINICAL RELEVANCE Results indicated histologically normal canine supraspinatus tendons have a trilaminar appearance on MRI images.					
30058853	8	88	theme	substance-to-deep	1063:1079	arg1	thickness					1088:1096	central substance-to-deep margin thickness	1055:1096	central substance-to-deep margin thickness	1055:1096	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	5	89	theme	Tissue	591:596	arg1	specimens					598:606	Tissue specimens	591:606	Tissue specimens	591:606	Tissue specimens were stained with various stains to determine tendon morphology and composition.					
30058853	12	90	theme	hyperintense	1753:1764	arg1	signal					1766:1771	a hyperintense signal	1751:1771	a hyperintense signal on MRI images	1751:1785	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	8	91	theme	thickness	1088:1096	arg1	ratios					1098:1103	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios	997:1103	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios	997:1103	The mean ± SD central substance-to-superficial margin and central substance-to-deep margin thickness ratios were 8.4 ± 1.2 and 9.0 ± 0.9, respectively; supraspinatus tendon-to-triceps brachii muscle signal intensity ratio was 1.3 ± 0.2; and tendon volume was 445 ± 20 mm3.					
30058853	1	92	from	features	145:152	arg1	dogs					193:196	nonlame dogs	185:196	nonlame dogs	185:196	OBJECTIVE To characterize the MRI and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	0	93	from	features	42:49	arg1	dogs					90:93	nonlame dogs	82:93	nonlame dogs	82:93	Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	6	94	theme	MRI	704:706	arg1	findings					708:715	MRI findings	704:715	MRI findings	704:715	Histologic and MRI findings were correlated and described.					
30058853	0	95	theme	resonance	9:17	arg1	imaging					19:25	Magnetic resonance imaging	0:25	Magnetic resonance imaging	0:25	Magnetic resonance imaging and histologic features of the supraspinatus tendon in nonlame dogs.					
30058853	7	96	theme	thin	950:953	arg1	margins					988:994	thin hypointense superficial and deep margins	950:994	thin hypointense superficial and deep margins	950:994	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	4	97	theme	shoulder	532:539	arg1	joints					541:546	the shoulder joints	528:546	the shoulder joints	528:546	After MRI scanning was complete, the shoulder joints were processed for histologic examination.					
30058853	7	98	theme	transverse	826:835	arg1	images					841:846	sagittal and transverse MRI images	813:846	sagittal and transverse MRI images	813:846	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	7	99	theme	sandwiched	931:940	arg1	layer					905:909	a thick, hyperintense center layer	876:909	a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins	876:994	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	7	99	theme	sandwiched	931:940	arg1	substance					920:928	central substance	912:928	central substance	912:928	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	7	100	theme	sagittal	813:820	arg1	images					841:846	sagittal and transverse MRI images	813:846	sagittal and transverse MRI images	813:846	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	12	101	contain	having	1744:1749	arg2	signal					1766:1771	a hyperintense signal	1751:1771	a hyperintense signal on MRI images	1751:1785	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	12	101	contain	having	1744:1749	arg1	tendon					1737:1742	the tendon	1733:1742	the tendon having a hyperintense signal on MRI images	1733:1785	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
30058853	3	102	theme	signal	434:439	arg1	intensity					441:449	signal intensity	434:449	signal intensity	434:449	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	7	103	theme	central	912:918	arg1	layer					905:909	a thick, hyperintense center layer	876:909	a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins	876:994	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	7	103	theme	central	912:918	arg1	substance					920:928	central substance	912:928	central substance	912:928	RESULTS All supraspinatus tendons had a trilaminar appearance on sagittal and transverse MRI images, which was characterized by a thick, hyperintense center layer (central substance) sandwiched between thin hypointense superficial and deep margins.					
30058853	10	104	theme	central	1383:1389	arg1	substance					1391:1399	The central substance	1379:1399	The central substance	1379:1399	The central substance was comprised of water-rich glycosaminoglycans interspersed among haphazardly arranged collagen bundles.					
30058853	3	105	theme	supraspinatus	459:471	arg1	tendon					473:478	each supraspinatus tendon	454:478	each supraspinatus tendon	454:478	PROCEDURES Multiple MRI fluid-sensitive pulse sequences were obtained for both shoulder joints of each cadaver, and the thickness, volume, and signal intensity of each supraspinatus tendon were assessed.					
30058853	12	106	theme	diagnostic	1837:1846	arg1	findings					1848:1855	diagnostic findings	1837:1855	diagnostic findings	1837:1855	In dogs, a diagnosis of supraspinatus tendinosis should not be based solely on the tendon having a hyperintense signal on MRI images; other MRI evidence of shoulder joint disease and diagnostic findings are necessary to support such a diagnosis.					
31664907	5	0	dep	internodes	803:812	arg1	reads					821:825	reads	821:825	reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species	821:948	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	11	1	theme	association	2066:2076	arg1	panels					2078:2083	association panels	2066:2083	association panels of inbreds and landraces	2066:2108	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	10	2	from	variation	1813:1821	arg1	maize					1826:1830	maize	1826:1830	maize as a species	1826:1843	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	2	3	from	source	307:312	arg1	residues					351:358	crop residues	346:358	crop residues	346:358	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	6	4	theme	co-expressed	1158:1169	arg1	genes					1171:1175	co-expressed genes	1158:1175	co-expressed genes	1158:1175	Using a slope-metric algorithm, five distinct patterns for sub-sets of co-expressed genes were defined across a time course of stem development.					
31664907	4	5	theme	developing	610:619	arg1	tissues					626:632	developing rind tissues	610:632	developing rind tissues of stem internodes	610:651	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	10	6	theme	cell	1876:1879	arg1	networks					1910:1917	cell wall-related gene expression networks	1876:1917	cell wall-related gene expression networks	1876:1917	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	2	7	theme	genetic	259:265	arg1	model					267:271	a genetic model	257:271	a genetic model for grass species	257:289	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	2	7	theme	genetic	259:265	arg1	Maize					243:247	Maize	243:247	Maize	243:247	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	4	8	theme	wall-related	696:707	arg1	genes					709:713	cell wall-related genes	691:713	cell wall-related genes	691:713	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	10	9	theme	networks	1910:1917	arg1	extrapolation					1859:1871	the extrapolation	1855:1871	the extrapolation of cell wall-related gene expression networks	1855:1917	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	6	10	theme	stem	1214:1217	arg1	development					1219:1229	stem development	1214:1229	stem development	1214:1229	Using a slope-metric algorithm, five distinct patterns for sub-sets of co-expressed genes were defined across a time course of stem development.					
31664907	11	11	theme	genotype-specific	1979:1995	arg1	patterns					2008:2015	genotype-specific expression patterns	1979:2015	genotype-specific expression patterns	1979:2015	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	2	12	from	residues	351:358	arg1	source					307:312	a potential source	295:312	a potential source of lignocellulosic biomass from crop residues	295:358	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	2	12	from	residues	351:358	arg1	biomass					333:339	lignocellulosic biomass	317:339	lignocellulosic biomass from crop residues	317:358	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	2	12	from	residues	351:358	arg1	model					267:271	a genetic model	257:271	a genetic model for grass species	257:289	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	2	12	from	residues	351:358	arg1	Maize					243:247	Maize	243:247	Maize	243:247	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	10	13	theme	gene	1894:1897	arg1	networks					1910:1917	cell wall-related gene expression networks	1876:1917	cell wall-related gene expression networks	1876:1917	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	11	14	theme	expression	1997:2006	arg1	patterns					2008:2015	genotype-specific expression patterns	1979:2015	genotype-specific expression patterns	1979:2015	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	4	15	theme	comprehensive	664:676	arg1	inventory					678:686	a comprehensive inventory	662:686	a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73)	662:747	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	10	16	theme	common	1933:1938	arg1	line					1947:1950	one common inbred line	1929:1950	one common inbred line to another	1929:1961	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	8	17	theme	5-fold	1540:1545	arg1	difference					1547:1556	at least a 5-fold difference	1529:1556	at least a 5-fold difference	1529:1556	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	8	18	theme	maize	1416:1420	arg1	B73					1431:1433	B73	1431:1433	B73	1431:1433	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	8	18	theme	maize	1416:1420	arg1	inbreds					1422:1428	two maize inbreds	1412:1428	two maize inbreds	1412:1428	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	8	18	theme	maize	1416:1420	arg1	Mo17					1439:1442	Mo17	1439:1442	Mo17	1439:1442	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	7	19	theme	promoter	1336:1343	arg1	regions					1345:1351	promoter regions	1336:1351	promoter regions	1336:1351	For the subset of genes associated with secondary wall formation, fifteen sequence motifs were found in promoter regions.					
31664907	5	20	theme	20 M.	871:875	arg1	Grasses					877:883	20 M. Grasses	871:883	20 M. Grasses	871:883	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	2	21	from	model	267:271	arg1	residues					351:358	crop residues	346:358	crop residues	346:358	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	11	22	theme	regulatory	2027:2036	arg1	controls					2038:2045	their regulatory controls	2021:2045	their regulatory controls	2021:2045	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	4	23	theme	cultivar	735:742	arg1	mays					729:732	Zea mays	725:732	Zea mays	725:732	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	4	23	theme	cultivar	735:742	arg1	B73					744:746	cultivar B73	735:746	cultivar B73	735:746	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	1	24	theme	cellular	133:140	arg1	machinery					142:150	The cellular machinery	129:150	The cellular machinery for cell wall synthesis and metabolism	129:189	BACKGROUND The cellular machinery for cell wall synthesis and metabolism is encoded by members of large multi-gene families.					
31664907	11	25	theme	grass	2174:2178	arg1	species					2180:2186	other bioenergy grass species	2158:2186	other bioenergy grass species	2158:2186	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	11	26	theme	other	2158:2162	arg1	species					2180:2186	other bioenergy grass species	2158:2186	other bioenergy grass species	2158:2186	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	0	27	theme	common	72:77	arg1	inbreds					85:91	two common maize inbreds	68:91	two common maize inbreds	68:91	Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development.					
31664907	5	28	from	≥500	856:859	arg1	20 M					831:834	20 M	831:834	20 M	831:834	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	5	28	from	≥500	856:859	arg1	693					841:843	693	841:843	693	841:843	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	1	29	theme	wall	161:164	arg1	synthesis					166:174	cell wall synthesis	156:174	cell wall synthesis	156:174	BACKGROUND The cellular machinery for cell wall synthesis and metabolism is encoded by members of large multi-gene families.					
31664907	9	30	theme	CADa	1701:1704	arg1	expression					1685:1694	expression	1685:1694	expression of a CADa	1685:1704	Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements.					
31664907	4	31	dep	maize	718:722	arg1	mays					729:732	Zea mays	725:732	Zea mays	725:732	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	4	31	dep	maize	718:722	arg1	B73					744:746	cultivar B73	735:746	cultivar B73	735:746	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	8	32	theme	gene	1459:1462	arg1	expression					1464:1473	gene expression	1459:1473	gene expression between them	1459:1486	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	0	33	theme	stem	100:103	arg1	development					105:115	stem development	100:115	stem development	100:115	Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development.					
31664907	7	34	theme	sequence	1306:1313	arg1	motifs					1315:1320	fifteen sequence motifs	1298:1320	fifteen sequence motifs	1298:1320	For the subset of genes associated with secondary wall formation, fifteen sequence motifs were found in promoter regions.					
31664907	11	35	theme	genetic	2127:2133	arg1	variation					2135:2143	genetic variation	2127:2143	genetic variation in maize and other bioenergy grass species	2127:2186	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	0	36	theme	Expression	0:9	arg1	profiles					11:18	Expression profiles	0:18	Expression profiles of cell-wall related genes	0:45	Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development.					
31664907	5	37	theme	wall	895:898	arg1	compositions					900:911	cell wall compositions	890:911	cell wall compositions distinct from non-commelinid species	890:948	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	3	38	theme	Genetic	361:367	arg1	improvement					369:379	Genetic improvement	361:379	Genetic improvement of maize for its utility as a bioenergy feedstock	361:429	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	5	39	theme	distinct	913:920	arg1	compositions					900:911	cell wall compositions	890:911	cell wall compositions distinct from non-commelinid species	890:948	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	7	40	theme	wall	1282:1285	arg1	formation					1287:1295	secondary wall formation	1272:1295	secondary wall formation	1272:1295	For the subset of genes associated with secondary wall formation, fifteen sequence motifs were found in promoter regions.					
31664907	6	41	theme	time	1199:1202	arg1	course					1204:1209	a time course	1197:1209	a time course of stem development	1197:1229	Using a slope-metric algorithm, five distinct patterns for sub-sets of co-expressed genes were defined across a time course of stem development.					
31664907	10	42	theme	CONCLUSIONS	1787:1797	arg1	variation					1813:1821	CONCLUSIONS Large genetic variation	1787:1821	CONCLUSIONS Large genetic variation in maize as a species	1787:1843	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	0	43	theme	related	33:39	arg1	genes					41:45	cell-wall related genes	23:45	cell-wall related genes	23:45	Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development.					
31664907	5	44	theme	wall-related	982:993	arg1	genes					995:999	maize cell wall-related genes	971:999	maize cell wall-related genes expressed in stems	971:1018	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	9	45	theme	promoter	1759:1766	arg1	elements					1777:1784	promoter response elements	1759:1784	promoter response elements	1759:1784	Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements.					
31664907	8	46	theme	gene	1374:1377	arg1	families					1379:1386	gene families	1374:1386	gene families	1374:1386	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	3	47	theme	family	478:483	arg1	members					485:491	the specific gene family members	460:491	the specific gene family members expressed during secondary wall development in stems	460:544	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	9	48	theme	copy-number	1602:1612	arg1	variation					1614:1622	copy-number variation	1602:1622	copy-number variation	1602:1622	Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements.					
31664907	6	49	theme	slope-metric	1095:1106	arg1	algorithm					1108:1116	a slope-metric algorithm	1093:1116	a slope-metric algorithm	1093:1116	Using a slope-metric algorithm, five distinct patterns for sub-sets of co-expressed genes were defined across a time course of stem development.					
31664907	3	50	theme	specific	464:471	arg1	members					485:491	the specific gene family members	460:491	the specific gene family members expressed during secondary wall development in stems	460:544	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	1	51	theme	large	216:220	arg1	families					233:240	large multi-gene families	216:240	large multi-gene families	216:240	BACKGROUND The cellular machinery for cell wall synthesis and metabolism is encoded by members of large multi-gene families.					
31664907	4	52	theme	rind	621:624	arg1	tissues					626:632	developing rind tissues	610:632	developing rind tissues of stem internodes	610:651	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	10	53	theme	genetic	1805:1811	arg1	variation					1813:1821	CONCLUSIONS Large genetic variation	1787:1821	CONCLUSIONS Large genetic variation in maize as a species	1787:1843	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	3	54	theme	secondary	510:518	arg1	development					525:535	secondary wall development	510:535	secondary wall development in stems	510:544	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	1	55	theme	families	233:240	arg1	members					205:211	members	205:211	members of large multi-gene families	205:240	BACKGROUND The cellular machinery for cell wall synthesis and metabolism is encoded by members of large multi-gene families.					
31664907	7	56	theme	genes	1250:1254	arg1	subset					1240:1245	the subset	1236:1245	the subset of genes associated with secondary wall formation	1236:1295	For the subset of genes associated with secondary wall formation, fifteen sequence motifs were found in promoter regions.					
31664907	2	57	theme	crop	346:349	arg1	residues					351:358	crop residues	346:358	crop residues	346:358	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	9	58	from	polymorphisms	1742:1754	arg1	elements					1777:1784	promoter response elements	1759:1784	promoter response elements	1759:1784	Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements.					
31664907	4	59	theme	transcripts	585:595	arg1	sequencing					571:580	High-throughput sequencing	555:580	High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes	555:651	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	2	60	theme	grass	277:281	arg1	species					283:289	grass species	277:289	grass species	277:289	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	2	61	theme	biomass	333:339	arg1	source					307:312	a potential source	295:312	a potential source of lignocellulosic biomass from crop residues	295:358	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	2	61	theme	biomass	333:339	arg1	model					267:271	a genetic model	257:271	a genetic model for grass species	257:289	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	2	61	theme	biomass	333:339	arg1	Maize					243:247	Maize	243:247	Maize	243:247	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	5	62	dep	expressed	783:791	arg1	orthologous					1036:1046	orthologous	1036:1046	orthologous	1036:1046	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	5	63	theme	genes	767:771	arg1	1239					753:756	1239	753:756	1239 of these genes	753:771	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	6	64	theme	development	1219:1229	arg1	course					1204:1209	a time course	1197:1209	a time course of stem development	1197:1229	Using a slope-metric algorithm, five distinct patterns for sub-sets of co-expressed genes were defined across a time course of stem development.					
31664907	2	65	theme	potential	297:305	arg1	source					307:312	a potential source	295:312	a potential source of lignocellulosic biomass from crop residues	295:358	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	4	66	from	inventory	678:686	arg1	maize					718:722	maize	718:722	maize (Zea mays, cultivar B73)	718:747	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	11	67	theme	inbreds	2088:2094	arg1	panels					2078:2083	association panels	2066:2083	association panels of inbreds and landraces	2066:2108	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	6	68	theme	distinct	1124:1131	arg1	patterns					1133:1140	five distinct patterns	1119:1140	five distinct patterns for sub-sets of co-expressed genes	1119:1175	Using a slope-metric algorithm, five distinct patterns for sub-sets of co-expressed genes were defined across a time course of stem development.					
31664907	4	69	theme	cell	691:694	arg1	genes					709:713	cell wall-related genes	691:713	cell wall-related genes	691:713	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	10	70	theme	expression	1899:1908	arg1	networks					1910:1917	cell wall-related gene expression networks	1876:1917	cell wall-related gene expression networks	1876:1917	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	4	71	theme	internodes	642:651	arg1	tissues					626:632	developing rind tissues	610:632	developing rind tissues of stem internodes	610:651	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	2	72	theme	lignocellulosic	317:331	arg1	biomass					333:339	lignocellulosic biomass	317:339	lignocellulosic biomass from crop residues	317:358	Maize is both a genetic model for grass species and a potential source of lignocellulosic biomass from crop residues.					
31664907	11	73	theme	patterns	2008:2015	arg1	Elucidation					1964:1974	Elucidation	1964:1974	Elucidation of genotype-specific expression patterns and their regulatory controls	1964:2045	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	5	74	contain	have	885:888	arg1	Grasses					877:883	20 M. Grasses	871:883	20 M. Grasses	871:883	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	5	74	contain	have	885:888	arg2	compositions					900:911	cell wall compositions	890:911	cell wall compositions distinct from non-commelinid species	890:948	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	4	75	theme	Zea	725:727	arg1	mays					729:732	Zea mays	725:732	Zea mays	725:732	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	4	75	theme	Zea	725:727	arg1	B73					744:746	cultivar B73	735:746	cultivar B73	735:746	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	11	76	theme	controls	2038:2045	arg1	Elucidation					1964:1974	Elucidation	1964:1974	Elucidation of genotype-specific expression patterns and their regulatory controls	1964:2045	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	5	77	theme	them	848:851	arg1	20 M					831:834	20 M	831:834	20 M	831:834	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	5	77	theme	them	848:851	arg1	693					841:843	693	841:843	693	841:843	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	9	78	theme	expression	1685:1694	arg1	gene					1718:1721	a FLA11 gene	1710:1721	a FLA11 gene	1710:1721	Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements.					
31664907	9	78	theme	expression	1685:1694	arg1	fold-changes					1669:1680	fold-changes	1669:1680	fold-changes of expression of a CADa	1669:1704	Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements.					
31664907	6	79	theme	genes	1171:1175	arg1	sub-sets					1146:1153	sub-sets	1146:1153	sub-sets of co-expressed genes	1146:1175	Using a slope-metric algorithm, five distinct patterns for sub-sets of co-expressed genes were defined across a time course of stem development.					
31664907	4	80	theme	genes	709:713	arg1	inventory					678:686	a comprehensive inventory	662:686	a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73)	662:747	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	3	81	from	development	525:535	arg1	stems					540:544	stems	540:544	stems	540:544	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	10	82	theme	inbred	1940:1945	arg1	line					1947:1950	one common inbred line	1929:1950	one common inbred line to another	1929:1961	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	0	83	theme	maize	79:83	arg1	inbreds					85:91	two common maize inbreds	68:91	two common maize inbreds	68:91	Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development.					
31664907	1	84	theme	cell	156:159	arg1	synthesis					166:174	cell wall synthesis	156:174	cell wall synthesis	156:174	BACKGROUND The cellular machinery for cell wall synthesis and metabolism is encoded by members of large multi-gene families.					
31664907	11	85	theme	bioenergy	2164:2172	arg1	species					2180:2186	other bioenergy grass species	2158:2186	other bioenergy grass species	2158:2186	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	10	86	theme	wall-related	1881:1892	arg1	networks					1910:1917	cell wall-related gene expression networks	1876:1917	cell wall-related gene expression networks	1876:1917	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	8	87	theme	expression	1464:1473	arg1	levels					1449:1454	levels	1449:1454	levels of gene expression between them	1449:1486	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	5	88	theme	non-commelinid	927:940	arg1	species					942:948	non-commelinid species	927:948	non-commelinid species	927:948	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	5	89	theme	eudicot	1066:1072	arg1	Arabidopsis					1074:1084	the eudicot Arabidopsis	1062:1084	the eudicot Arabidopsis	1062:1084	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	9	90	theme	FLA11	1712:1716	arg1	gene					1718:1721	a FLA11 gene	1710:1721	a FLA11 gene	1710:1721	Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements.					
31664907	11	91	theme	landraces	2100:2108	arg1	panels					2078:2083	association panels	2066:2083	association panels of inbreds and landraces	2066:2108	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	1	92	dep	BACKGROUND	118:127	arg1	encoded					194:200	encoded	194:200	is encoded by members of large multi-gene families	191:240	BACKGROUND The cellular machinery for cell wall synthesis and metabolism is encoded by members of large multi-gene families.					
31664907	4	93	dep	RESULTS	547:553	arg1	provided					653:660	provided	653:660	provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73)	653:747	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	5	94	theme	cell	890:893	arg1	compositions					900:911	cell wall compositions	890:911	cell wall compositions distinct from non-commelinid species	890:948	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	3	95	theme	maize	384:388	arg1	improvement					369:379	Genetic improvement	361:379	Genetic improvement of maize for its utility as a bioenergy feedstock	361:429	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	4	96	theme	High-throughput	555:569	arg1	sequencing					571:580	High-throughput sequencing	555:580	High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes	555:651	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	3	97	theme	bioenergy	411:419	arg1	feedstock					421:429	a bioenergy feedstock	409:429	a bioenergy feedstock	409:429	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	0	98	theme	cell-wall	23:31	arg1	genes					41:45	cell-wall related genes	23:45	cell-wall related genes	23:45	Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development.					
31664907	7	99	theme	secondary	1272:1280	arg1	formation					1287:1295	secondary wall formation	1272:1295	secondary wall formation	1272:1295	For the subset of genes associated with secondary wall formation, fifteen sequence motifs were found in promoter regions.					
31664907	10	100	theme	Large	1799:1803	arg1	variation					1813:1821	CONCLUSIONS Large genetic variation	1787:1821	CONCLUSIONS Large genetic variation in maize as a species	1787:1843	CONCLUSIONS Large genetic variation in maize as a species precludes the extrapolation of cell wall-related gene expression networks even from one common inbred line to another.					
31664907	5	101	with	orthologous	1036:1046	arg1	those					1053:1057	those	1053:1057	those	1053:1057	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	0	102	theme	genes	41:45	arg1	profiles					11:18	Expression profiles	0:18	Expression profiles of cell-wall related genes	0:45	Expression profiles of cell-wall related genes vary broadly between two common maize inbreds during stem development.					
31664907	5	103	theme	cell	977:980	arg1	genes					995:999	maize cell wall-related genes	971:999	maize cell wall-related genes expressed in stems	971:1018	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	8	104	theme	same	1358:1361	arg1	members					1363:1369	The same members	1354:1369	The same members of gene families	1354:1386	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	1	105	theme	multi-gene	222:231	arg1	families					233:240	large multi-gene families	216:240	large multi-gene families	216:240	BACKGROUND The cellular machinery for cell wall synthesis and metabolism is encoded by members of large multi-gene families.					
31664907	5	106	theme	genes	995:999	arg1	one-quarter					956:966	one-quarter	956:966	one-quarter of maize cell wall-related genes expressed in stems	956:1018	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	9	107	theme	response	1768:1775	arg1	elements					1777:1784	promoter response elements	1759:1784	promoter response elements	1759:1784	Although presence-absence and copy-number variation might account for much of these differences, fold-changes of expression of a CADa and a FLA11 gene were attributed to polymorphisms in promoter response elements.					
31664907	8	108	theme	genes	1512:1516	arg1	%					1503:1503	30%	1501:1503	30% of all genes exhibiting at least a 5-fold difference at any stage	1501:1569	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	8	108	theme	genes	1512:1516	arg1	genes					1512:1516	all genes	1508:1516	all genes exhibiting at least a 5-fold difference at any stage	1508:1569	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	3	109	theme	members	485:491	arg1	identification					442:455	identification	442:455	identification of the specific gene family members expressed during secondary wall development in stems	442:544	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	5	110	theme	maize	971:975	arg1	genes					995:999	maize cell wall-related genes	971:999	maize cell wall-related genes expressed in stems	971:1018	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31664907	8	111	theme	families	1379:1386	arg1	members					1363:1369	The same members	1354:1369	The same members of gene families	1354:1386	The same members of gene families were often expressed in two maize inbreds, B73 and Mo17, but levels of gene expression between them varied, with 30% of all genes exhibiting at least a 5-fold difference at any stage.					
31664907	3	112	theme	gene	473:476	arg1	members					485:491	the specific gene family members	460:491	the specific gene family members expressed during secondary wall development in stems	460:544	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	7	113	located	found	1327:1331	arg1	regions					1345:1351	promoter regions	1336:1351	promoter regions	1336:1351	For the subset of genes associated with secondary wall formation, fifteen sequence motifs were found in promoter regions.					
31664907	7	113	located	found	1327:1331	arg2	motifs					1315:1320	fifteen sequence motifs	1298:1320	fifteen sequence motifs	1298:1320	For the subset of genes associated with secondary wall formation, fifteen sequence motifs were found in promoter regions.					
31664907	4	114	theme	stem	637:640	arg1	internodes					642:651	stem internodes	637:651	stem internodes	637:651	RESULTS High-throughput sequencing of transcripts expressed in developing rind tissues of stem internodes provided a comprehensive inventory of cell wall-related genes in maize (Zea mays, cultivar B73).					
31664907	11	115	from	variation	2135:2143	arg1	species					2180:2186	other bioenergy grass species	2158:2186	other bioenergy grass species	2158:2186	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	11	115	from	variation	2135:2143	arg1	maize					2148:2152	maize	2148:2152	maize	2148:2152	Elucidation of genotype-specific expression patterns and their regulatory controls will be needed for association panels of inbreds and landraces to fully exploit genetic variation in maize and other bioenergy grass species.					
31664907	3	116	theme	wall	520:523	arg1	development					525:535	secondary wall development	510:535	secondary wall development in stems	510:544	Genetic improvement of maize for its utility as a bioenergy feedstock depends on identification of the specific gene family members expressed during secondary wall development in stems.					
31664907	5	117	from	species	942:948	arg1	distinct					913:920	distinct	913:920	distinct	913:920	Of 1239 of these genes, 854 were expressed among the internodes at ≥95 reads per 20 M, and 693 of them at ≥500 reads per 20 M. Grasses have cell wall compositions distinct from non-commelinid species; only one-quarter of maize cell wall-related genes expressed in stems were putatively orthologous with those of the eudicot Arabidopsis.					
31121968	7	0	theme	bacteria	1253:1260	arg1	attachment					1262:1271	the bacteria attachment	1249:1271	the bacteria attachment on the coating surface	1249:1294	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	6	1	theme	Zn-Ni	944:948	arg1	coating					956:962	the Zn-Ni alloy coating	940:962	the Zn-Ni alloy coating	940:962	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	2	2	theme	corrosive	356:364	arg1	conditions					366:375	microbial corrosive conditions	346:375	microbial corrosive conditions	346:375	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	8	3	dep	anticorrosive	1440:1452	arg1	enhanced					1431:1438	enhanced	1431:1438	enhanced	1431:1438	As a result, the Zn-Ni-chitosan coatings showed remarkably enhanced anticorrosive and antibacterial properties.					
31121968	2	4	theme	microbial	346:354	arg1	conditions					366:375	microbial corrosive conditions	346:375	microbial corrosive conditions	346:375	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	8	5	theme	anticorrosive	1440:1452	arg1	result					1377:1382	a result	1375:1382	a result	1375:1382	As a result, the Zn-Ni-chitosan coatings showed remarkably enhanced anticorrosive and antibacterial properties.					
31121968	8	5	theme	anticorrosive	1440:1452	arg1	properties					1472:1481	remarkably enhanced anticorrosive and antibacterial properties	1420:1481	remarkably enhanced anticorrosive and antibacterial properties	1420:1481	As a result, the Zn-Ni-chitosan coatings showed remarkably enhanced anticorrosive and antibacterial properties.					
31121968	2	6	theme	Zn-Ni	292:296	arg1	coating					304:310	the Zn-Ni alloy coating	288:310	the Zn-Ni alloy coating	288:310	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	1	7	theme	universal	128:136	arg1	phenomenon					138:147	a universal phenomenon	126:147	a universal phenomenon in salt water media such as seawater and wastewater environments	126:212	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	1	7	theme	universal	128:136	arg1	corrosion					113:121	Microbial corrosion	103:121	Microbial corrosion	103:121	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	6	8	theme	sulfate-reducing	1026:1041	arg1	bacteria					1043:1050	sulfate-reducing bacteria	1026:1050	sulfate-reducing bacteria (SRB)	1026:1056	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	6	8	theme	sulfate-reducing	1026:1041	arg1	SRB					1053:1055	SRB	1053:1055	SRB	1053:1055	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	7	9	theme	coating	1280:1286	arg1	surface					1288:1294	the coating surface	1276:1294	the coating surface	1276:1294	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	4	10	theme	phase	738:742	arg1	composition					744:754	the phase composition	734:754	the phase composition	734:754	It was found that the addition of chitosan significantly and negatively shifted the electrodeposition potentials and influenced the Ni contents, the phase composition, and the surface morphologies.					
31121968	4	11	theme	Ni	721:722	arg1	contents					724:731	the Ni contents	717:731	the Ni contents	717:731	It was found that the addition of chitosan significantly and negatively shifted the electrodeposition potentials and influenced the Ni contents, the phase composition, and the surface morphologies.					
31121968	5	12	theme	microbial	855:863	arg1	resistance					875:884	the microbial corrosion resistance	851:884	the microbial corrosion resistance	851:884	By exposing the coatings in a sulfate-reducing bacteria medium, the microbial corrosion resistance was investigated.					
31121968	4	13	theme	electrodeposition	673:689	arg1	potentials					691:700	the electrodeposition potentials	669:700	the electrodeposition potentials	669:700	It was found that the addition of chitosan significantly and negatively shifted the electrodeposition potentials and influenced the Ni contents, the phase composition, and the surface morphologies.					
31121968	7	14	theme	Further	1131:1137	arg1	research					1139:1146	Further research	1131:1146	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium	1131:1235	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	1	15	theme	Microbial	103:111	arg1	phenomenon					138:147	a universal phenomenon	126:147	a universal phenomenon in salt water media such as seawater and wastewater environments	126:212	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	1	15	theme	Microbial	103:111	arg1	corrosion					113:121	Microbial corrosion	103:121	Microbial corrosion	103:121	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	1	16	theme	wastewater	190:199	arg1	environments					201:212	wastewater environments	190:212	wastewater environments	190:212	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	0	17	theme	Corrosion	10:18	arg1	Resistance					20:29	Microbial Corrosion Resistance	0:29	Microbial Corrosion Resistance	0:29	Microbial Corrosion Resistance and Antibacterial Property of Electrodeposited Zn-Ni-Chitosan Coatings.					
31121968	7	18	theme	enhanced	1337:1344	arg1	properties					1360:1369	enhanced antibacterial properties	1337:1369	enhanced antibacterial properties	1337:1369	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	3	19	theme	coatings	579:586	arg1	ingredient					533:542	a functional ingredient	520:542	a functional ingredient of electrodeposited Zn-Ni-chitosan coatings	520:586	To solve this problem, chitosan, which is considered a natural product with high antibacterial efficiency, was added to Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings.					
31121968	7	20	theme	buffer	1216:1221	arg1	medium					1230:1235	an Escherichia coli-suspended phosphate buffer saline medium	1176:1235	an Escherichia coli-suspended phosphate buffer saline medium	1176:1235	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	0	21	theme	Microbial	0:8	arg1	Resistance					20:29	Microbial Corrosion Resistance	0:29	Microbial Corrosion Resistance	0:29	Microbial Corrosion Resistance and Antibacterial Property of Electrodeposited Zn-Ni-Chitosan Coatings.					
31121968	1	22	from	phenomenon	138:147	arg1	environments					201:212	wastewater environments	190:212	wastewater environments	190:212	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	1	22	from	phenomenon	138:147	arg1	media					163:167	salt water media	152:167	salt water media such as seawater and wastewater environments	152:212	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	1	22	from	phenomenon	138:147	arg1	seawater					177:184	seawater	177:184	seawater	177:184	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	6	23	theme	coatings	1091:1098	arg1	rates					1076:1080	the corrosion rates	1062:1080	the corrosion rates of these coatings	1062:1098	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	7	24	theme	coli-suspended	1191:1204	arg1	buffer					1216:1221	an Escherichia coli-suspended phosphate buffer	1176:1221	an Escherichia coli-suspended phosphate buffer saline medium	1176:1235	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	5	25	theme	bacteria	834:841	arg1	medium					843:848	a sulfate-reducing bacteria medium	815:848	a sulfate-reducing bacteria medium	815:848	By exposing the coatings in a sulfate-reducing bacteria medium, the microbial corrosion resistance was investigated.					
31121968	3	26	with	product	441:447	arg1	efficiency					473:482	high antibacterial efficiency	454:482	high antibacterial efficiency	454:482	To solve this problem, chitosan, which is considered a natural product with high antibacterial efficiency, was added to Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings.					
31121968	2	27	theme	coating	304:310	arg1	damage					278:283	the damage	274:283	the damage of the Zn-Ni alloy coating	274:310	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	2	27	theme	coating	304:310	arg1	kind					220:223	a kind	218:223	a kind of efficient protective metal coating for steel	218:271	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	3	28	theme	high	454:457	arg1	efficiency					473:482	high antibacterial efficiency	454:482	high antibacterial efficiency	454:482	To solve this problem, chitosan, which is considered a natural product with high antibacterial efficiency, was added to Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings.					
31121968	7	29	theme	phosphate	1206:1214	arg1	buffer					1216:1221	an Escherichia coli-suspended phosphate buffer	1176:1221	an Escherichia coli-suspended phosphate buffer saline medium	1176:1235	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	6	30	theme	obvious	996:1002	arg1	effects					1015:1021	obvious inhibiting effects	996:1021	obvious inhibiting effects on sulfate-reducing bacteria (SRB)	996:1056	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	3	31	theme	Zn-Ni-chitosan	564:577	arg1	coatings					579:586	electrodeposited Zn-Ni-chitosan coatings	547:586	electrodeposited Zn-Ni-chitosan coatings	547:586	To solve this problem, chitosan, which is considered a natural product with high antibacterial efficiency, was added to Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings.					
31121968	3	32	theme	antibacterial	459:471	arg1	efficiency					473:482	high antibacterial efficiency	454:482	high antibacterial efficiency	454:482	To solve this problem, chitosan, which is considered a natural product with high antibacterial efficiency, was added to Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings.					
31121968	7	33	theme	antibacterial	1346:1358	arg1	properties					1360:1369	enhanced antibacterial properties	1337:1369	enhanced antibacterial properties	1337:1369	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	6	34	from	effects	1015:1021	arg1	bacteria					1043:1050	sulfate-reducing bacteria	1026:1050	sulfate-reducing bacteria (SRB)	1026:1056	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	6	34	from	effects	1015:1021	arg1	SRB					1053:1055	SRB	1053:1055	SRB	1053:1055	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	7	35	theme	saline	1223:1228	arg1	medium					1230:1235	an Escherichia coli-suspended phosphate buffer saline medium	1176:1235	an Escherichia coli-suspended phosphate buffer saline medium	1176:1235	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	2	36	theme	coating	255:261	arg1	damage					278:283	the damage	274:283	the damage of the Zn-Ni alloy coating	274:310	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	2	36	theme	coating	255:261	arg1	kind					220:223	a kind	218:223	a kind of efficient protective metal coating for steel	218:271	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	5	37	theme	corrosion	865:873	arg1	resistance					875:884	the microbial corrosion resistance	851:884	the microbial corrosion resistance	851:884	By exposing the coatings in a sulfate-reducing bacteria medium, the microbial corrosion resistance was investigated.					
31121968	0	38	theme	Antibacterial	35:47	arg1	Property					49:56	Antibacterial Property	35:56	Antibacterial Property	35:56	Microbial Corrosion Resistance and Antibacterial Property of Electrodeposited Zn-Ni-Chitosan Coatings.					
31121968	7	39	from	research	1139:1146	arg1	coatings					1155:1162	the coatings	1151:1162	the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium	1151:1235	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	7	40	theme	Escherichia	1179:1189	arg1	buffer					1216:1221	an Escherichia coli-suspended phosphate buffer	1176:1221	an Escherichia coli-suspended phosphate buffer saline medium	1176:1235	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	2	41	theme	metal	249:253	arg1	coating					255:261	efficient protective metal coating	228:261	efficient protective metal coating for steel	228:271	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	4	42	theme	surface	765:771	arg1	morphologies					773:784	the surface morphologies	761:784	the surface morphologies	761:784	It was found that the addition of chitosan significantly and negatively shifted the electrodeposition potentials and influenced the Ni contents, the phase composition, and the surface morphologies.					
31121968	0	43	theme	Electrodeposited	61:76	arg1	Coatings					93:100	Electrodeposited Zn-Ni-Chitosan Coatings	61:100	Electrodeposited Zn-Ni-Chitosan Coatings	61:100	Microbial Corrosion Resistance and Antibacterial Property of Electrodeposited Zn-Ni-Chitosan Coatings.					
31121968	6	44	theme	Zn-Ni-chitosan	965:978	arg1	coatings					980:987	Zn-Ni-chitosan coatings	965:987	Zn-Ni-chitosan coatings	965:987	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	6	45	theme	inhibiting	1004:1013	arg1	effects					1015:1021	obvious inhibiting effects	996:1021	obvious inhibiting effects on sulfate-reducing bacteria (SRB)	996:1056	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	2	46	theme	protective	238:247	arg1	coating					255:261	efficient protective metal coating	228:261	efficient protective metal coating for steel	228:271	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	2	47	theme	efficient	228:236	arg1	coating					255:261	efficient protective metal coating	228:261	efficient protective metal coating for steel	228:271	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	3	48	theme	Zn-Ni	498:502	arg1	electrolytes					504:515	Zn-Ni electrolytes	498:515	Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings	498:586	To solve this problem, chitosan, which is considered a natural product with high antibacterial efficiency, was added to Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings.					
31121968	2	49	theme	alloy	298:302	arg1	coating					304:310	the Zn-Ni alloy coating	288:310	the Zn-Ni alloy coating	288:310	As a kind of efficient protective metal coating for steel, the damage of the Zn-Ni alloy coating was found to be accelerated under microbial corrosive conditions.					
31121968	7	50	from	attachment	1262:1271	arg1	surface					1288:1294	the coating surface	1276:1294	the coating surface	1276:1294	Further research on the coatings immersed in an Escherichia coli-suspended phosphate buffer saline medium showed that the bacteria attachment on the coating surface was effectively reduced, which indicated enhanced antibacterial properties.					
31121968	8	51	theme	antibacterial	1458:1470	arg1	result					1377:1382	a result	1375:1382	a result	1375:1382	As a result, the Zn-Ni-chitosan coatings showed remarkably enhanced anticorrosive and antibacterial properties.					
31121968	8	51	theme	antibacterial	1458:1470	arg1	properties					1472:1481	remarkably enhanced anticorrosive and antibacterial properties	1420:1481	remarkably enhanced anticorrosive and antibacterial properties	1420:1481	As a result, the Zn-Ni-chitosan coatings showed remarkably enhanced anticorrosive and antibacterial properties.					
31121968	1	52	theme	salt	152:155	arg1	environments					201:212	wastewater environments	190:212	wastewater environments	190:212	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	1	52	theme	salt	152:155	arg1	media					163:167	salt water media	152:167	salt water media such as seawater and wastewater environments	152:212	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	1	52	theme	salt	152:155	arg1	seawater					177:184	seawater	177:184	seawater	177:184	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	3	53	theme	electrodeposited	547:562	arg1	coatings					579:586	electrodeposited Zn-Ni-chitosan coatings	547:586	electrodeposited Zn-Ni-chitosan coatings	547:586	To solve this problem, chitosan, which is considered a natural product with high antibacterial efficiency, was added to Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings.					
31121968	3	54	theme	functional	522:531	arg1	ingredient					533:542	a functional ingredient	520:542	a functional ingredient of electrodeposited Zn-Ni-chitosan coatings	520:586	To solve this problem, chitosan, which is considered a natural product with high antibacterial efficiency, was added to Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings.					
31121968	8	55	theme	Zn-Ni-chitosan	1389:1402	arg1	coatings					1404:1411	the Zn-Ni-chitosan coatings	1385:1411	the Zn-Ni-chitosan coatings	1385:1411	As a result, the Zn-Ni-chitosan coatings showed remarkably enhanced anticorrosive and antibacterial properties.					
31121968	1	56	theme	water	157:161	arg1	environments					201:212	wastewater environments	190:212	wastewater environments	190:212	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	1	56	theme	water	157:161	arg1	media					163:167	salt water media	152:167	salt water media such as seawater and wastewater environments	152:212	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	1	56	theme	water	157:161	arg1	seawater					177:184	seawater	177:184	seawater	177:184	Microbial corrosion is a universal phenomenon in salt water media such as seawater and wastewater environments.					
31121968	0	57	theme	Coatings	93:100	arg1	Resistance					20:29	Microbial Corrosion Resistance	0:29	Microbial Corrosion Resistance	0:29	Microbial Corrosion Resistance and Antibacterial Property of Electrodeposited Zn-Ni-Chitosan Coatings.					
31121968	0	57	theme	Coatings	93:100	arg1	Property					49:56	Antibacterial Property	35:56	Antibacterial Property	35:56	Microbial Corrosion Resistance and Antibacterial Property of Electrodeposited Zn-Ni-Chitosan Coatings.					
31121968	6	58	theme	corrosion	1066:1074	arg1	rates					1076:1080	the corrosion rates	1062:1080	the corrosion rates of these coatings	1062:1098	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	3	59	theme	natural	433:439	arg1	product					441:447	a natural product	431:447	a natural product with high antibacterial efficiency	431:482	To solve this problem, chitosan, which is considered a natural product with high antibacterial efficiency, was added to Zn-Ni electrolytes as a functional ingredient of electrodeposited Zn-Ni-chitosan coatings.					
31121968	4	60	theme	chitosan	623:630	arg1	addition					611:618	the addition	607:618	the addition of chitosan	607:630	It was found that the addition of chitosan significantly and negatively shifted the electrodeposition potentials and influenced the Ni contents, the phase composition, and the surface morphologies.					
31121968	0	61	theme	Zn-Ni-Chitosan	78:91	arg1	Coatings					93:100	Electrodeposited Zn-Ni-Chitosan Coatings	61:100	Electrodeposited Zn-Ni-Chitosan Coatings	61:100	Microbial Corrosion Resistance and Antibacterial Property of Electrodeposited Zn-Ni-Chitosan Coatings.					
31121968	6	62	theme	alloy	950:954	arg1	coating					956:962	the Zn-Ni alloy coating	940:962	the Zn-Ni alloy coating	940:962	The results showed that compared to the Zn-Ni alloy coating, Zn-Ni-chitosan coatings showed obvious inhibiting effects on sulfate-reducing bacteria (SRB) and the corrosion rates of these coatings were mitigated to some degree.					
31121968	5	63	theme	sulfate-reducing	817:832	arg1	medium					843:848	a sulfate-reducing bacteria medium	815:848	a sulfate-reducing bacteria medium	815:848	By exposing the coatings in a sulfate-reducing bacteria medium, the microbial corrosion resistance was investigated.					
30423746	5	0	theme	blood	1266:1270	arg1	suspensions					1290:1300	blood system injectable suspensions	1266:1300	blood system injectable suspensions	1266:1300	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	4	1	theme	structural	1100:1109	arg1	analyses					1129:1136	structural and compositional analyses	1100:1136	structural and compositional analyses (FT-IR, TEM, XRD)	1100:1154	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	4	2	theme	NMR	960:962	arg1	analyses					972:979	NMR and DSC analyses	960:979	analyses	972:979	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	4	3	theme	DSC	968:970	arg1	analyses					972:979	NMR and DSC analyses	960:979	analyses	972:979	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	3	4	with	complex	775:781	arg1	sulfobutylether-β-cyclodextrin					796:825	anionic sulfobutylether-β-cyclodextrin	788:825	anionic sulfobutylether-β-cyclodextrin	788:825	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	2	5	theme	core-shell	366:375	arg1	nanoparticles					386:398	core-shell magnetic nanoparticles	366:398	core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action	366:499	The purpose of this study was to design, synthesize, and characterize core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action.					
30423746	3	6	theme	inclusion	765:773	arg1	complex					775:781	the protocatechuic acid or its inclusion complex	734:781	the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin	734:825	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	4	7	dep	analyses	1129:1136	arg1	TEM					1146:1148	TEM	1146:1148	TEM	1146:1148	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	4	7	dep	analyses	1129:1136	arg1	FT-IR					1139:1143	FT-IR	1139:1143	FT-IR	1139:1143	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	4	7	dep	analyses	1129:1136	arg1	XRD					1151:1153	XRD	1151:1153	XRD	1151:1153	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	1	8	theme	Core-shell	77:86	arg1	synthesis					110:118	Core-shell magnetic nanoparticle synthesis	77:118	Core-shell magnetic nanoparticle synthesis	77:118	Core-shell magnetic nanoparticle synthesis offers the opportunity to engineering their physical properties for specific applications when the intrinsic magnetic properties can be associated with other interesting ones.					
30423746	3	9	theme	magnetite	902:910	arg1	properties					884:893	the useful properties	873:893	the useful properties of the magnetite and the protocatechuic acid antioxidant effect	873:957	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	6	10	theme	biological	1663:1672	arg1	ability					1674:1680	their biological ability	1657:1680	their biological ability	1657:1680	Moreover, we proposed a nanocarrier that would be able to load unstable active principles and with very low solubility in biological fluids to increase their biological ability.					
30423746	3	11	theme	magnetite	603:611	arg1	nanoparticles					613:625	magnetite nanoparticles	603:625	magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery	603:850	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	1	12	theme	magnetic	88:95	arg1	synthesis					110:118	Core-shell magnetic nanoparticle synthesis	77:118	Core-shell magnetic nanoparticle synthesis	77:118	Core-shell magnetic nanoparticle synthesis offers the opportunity to engineering their physical properties for specific applications when the intrinsic magnetic properties can be associated with other interesting ones.					
30423746	4	13	theme	compositional	1115:1127	arg1	analyses					1129:1136	structural and compositional analyses	1100:1136	structural and compositional analyses (FT-IR, TEM, XRD)	1100:1154	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	5	14	theme	DPPH	1331:1334	arg1	test					1336:1339	DPPH test	1331:1339	DPPH test	1331:1339	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	3	15	theme	active	831:836	arg1	delivery					843:850	active drug delivery	831:850	active drug delivery	831:850	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	1	16	theme	nanoparticle	97:108	arg1	synthesis					110:118	Core-shell magnetic nanoparticle synthesis	77:118	Core-shell magnetic nanoparticle synthesis	77:118	Core-shell magnetic nanoparticle synthesis offers the opportunity to engineering their physical properties for specific applications when the intrinsic magnetic properties can be associated with other interesting ones.					
30423746	4	17	theme	magnetic	1202:1209	arg1	systems					1211:1217	the multifunctional magnetic systems	1182:1217	the multifunctional magnetic systems	1182:1217	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	4	18	theme	protocatechuic	1073:1086	arg1	acid					1088:1091	protocatechuic acid	1073:1091	protocatechuic acid	1073:1091	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	3	19	theme	anionic	788:794	arg1	sulfobutylether-β-cyclodextrin					796:825	anionic sulfobutylether-β-cyclodextrin	788:825	anionic sulfobutylether-β-cyclodextrin	788:825	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	2	20	theme	study	316:320	arg1	purpose					300:306	The purpose	296:306	The purpose of this study	296:320	The purpose of this study was to design, synthesize, and characterize core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action.					
30423746	1	21	theme	intrinsic	219:227	arg1	properties					238:247	the intrinsic magnetic properties	215:247	the intrinsic magnetic properties	215:247	Core-shell magnetic nanoparticle synthesis offers the opportunity to engineering their physical properties for specific applications when the intrinsic magnetic properties can be associated with other interesting ones.					
30423746	0	22	theme	magnetic	16:23	arg1	cargo-complexes					25:39	Multifunctional magnetic cargo-complexes	0:39	Multifunctional magnetic cargo-complexes with radical scavenging properties	0:74	Multifunctional magnetic cargo-complexes with radical scavenging properties.					
30423746	4	23	dep	confirmed	981:989	arg1	while					1094:1098	while	1094:1098	while	1094:1098	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	5	24	theme	dermal	1392:1397	arg1	cells					1411:1415	normal human dermal fibroblasts cells	1379:1415	normal human dermal fibroblasts cells	1379:1415	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	1	25	theme	magnetic	229:236	arg1	properties					238:247	the intrinsic magnetic properties	215:247	the intrinsic magnetic properties	215:247	Core-shell magnetic nanoparticle synthesis offers the opportunity to engineering their physical properties for specific applications when the intrinsic magnetic properties can be associated with other interesting ones.					
30423746	0	26	theme	Multifunctional	0:14	arg1	cargo-complexes					25:39	Multifunctional magnetic cargo-complexes	0:39	Multifunctional magnetic cargo-complexes with radical scavenging properties	0:74	Multifunctional magnetic cargo-complexes with radical scavenging properties.					
30423746	5	27	theme	injectable	1279:1288	arg1	suspensions					1290:1300	blood system injectable suspensions	1266:1300	blood system injectable suspensions	1266:1300	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	6	28	with	able	1555:1558	arg1	solubility					1613:1622	very low solubility	1604:1622	very low solubility in biological fluids	1604:1643	Moreover, we proposed a nanocarrier that would be able to load unstable active principles and with very low solubility in biological fluids to increase their biological ability.					
30423746	3	29	theme	branched	647:654	arg1	polyethyleneimine					656:672	branched polyethyleneimine	647:672	branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery	647:850	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	5	30	theme	fibroblasts	1399:1409	arg1	cells					1411:1415	normal human dermal fibroblasts cells	1379:1415	normal human dermal fibroblasts cells	1379:1415	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	5	31	dep	cytotoxicity	1346:1357	arg1	using					1360:1364	using	1360:1364	using MTS assay on normal human dermal fibroblasts cells	1360:1415	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	6	32	from	solubility	1613:1622	arg1	fluids					1638:1643	biological fluids	1627:1643	biological fluids	1627:1643	Moreover, we proposed a nanocarrier that would be able to load unstable active principles and with very low solubility in biological fluids to increase their biological ability.					
30423746	4	33	theme	inclusion	1012:1020	arg1	complex					1022:1028	the inclusion complex	1008:1028	the inclusion complex	1008:1028	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	5	34	from	assay	1370:1374	arg1	cells					1411:1415	normal human dermal fibroblasts cells	1379:1415	normal human dermal fibroblasts cells	1379:1415	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	4	35	theme	systems	1211:1217	arg1	synthesis					1169:1177	the synthesis	1165:1177	the synthesis of the multifunctional magnetic systems	1165:1217	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	2	36	theme	therapeutic	482:492	arg1	action					494:499	guidance and therapeutic action	469:499	guidance and therapeutic action	469:499	The purpose of this study was to design, synthesize, and characterize core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action.					
30423746	2	37	theme	action	494:499	arg1	possibility					454:464	the possibility	450:464	the possibility of guidance and therapeutic action	450:499	The purpose of this study was to design, synthesize, and characterize core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action.					
30423746	3	38	theme	drug	838:841	arg1	delivery					843:850	active drug delivery	831:850	active drug delivery	831:850	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	39	theme	nanocarriers	577:588	arg1	characterization					553:568	characterization	553:568	characterization	553:568	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	39	theme	nanocarriers	577:588	arg1	synthesis					539:547	synthesis	539:547	synthesis	539:547	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	40	theme	first	523:527	arg1	time					529:532	the first time	519:532	the first time	519:532	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	0	41	theme	scavenging	54:63	arg1	properties					65:74	radical scavenging properties	46:74	radical scavenging properties	46:74	Multifunctional magnetic cargo-complexes with radical scavenging properties.					
30423746	5	42	theme	antioxidant	1303:1313	arg1	activity					1315:1322	antioxidant activity	1303:1322	antioxidant activity (using DPPH test)	1303:1340	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	5	43	theme	MTS	1366:1368	arg1	assay					1370:1374	MTS assay	1366:1374	MTS assay on normal human dermal fibroblasts cells	1366:1415	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	3	44	theme	low	677:679	arg1	1.8 kDa					699:705	1.8 kDa	699:705	1.8 kDa	699:705	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	44	theme	low	677:679	arg1	weight					691:696	low molecular weight	677:696	low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery	677:850	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	4	45	theme	multifunctional	1186:1200	arg1	systems					1211:1217	the multifunctional magnetic systems	1182:1217	the multifunctional magnetic systems	1182:1217	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	2	46	theme	guidance	469:476	arg1	action					494:499	guidance and therapeutic action	469:499	guidance and therapeutic action	469:499	The purpose of this study was to design, synthesize, and characterize core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action.					
30423746	0	47	theme	radical	46:52	arg1	properties					65:74	radical scavenging properties	46:74	radical scavenging properties	46:74	Multifunctional magnetic cargo-complexes with radical scavenging properties.					
30423746	3	48	theme	protocatechuic	920:933	arg1	acid					935:938	the protocatechuic acid	916:938	the protocatechuic acid	916:938	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	49	theme	molecular	681:689	arg1	1.8 kDa					699:705	1.8 kDa	699:705	1.8 kDa	699:705	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	49	theme	molecular	681:689	arg1	weight					691:696	low molecular weight	677:696	low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery	677:850	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	6	50	theme	unstable	1568:1575	arg1	principles					1584:1593	load unstable active principles	1563:1593	load unstable active principles	1563:1593	Moreover, we proposed a nanocarrier that would be able to load unstable active principles and with very low solubility in biological fluids to increase their biological ability.					
30423746	0	51	with	cargo-complexes	25:39	arg1	properties					65:74	radical scavenging properties	46:74	radical scavenging properties	46:74	Multifunctional magnetic cargo-complexes with radical scavenging properties.					
30423746	4	52	theme	complex	1022:1028	arg1	formation					995:1003	the formation	991:1003	the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid	991:1091	NMR and DSC analyses confirmed the formation of the inclusion complex between sulfobutylether-β-cyclodextrin and protocatechuic acid, while structural and compositional analyses (FT-IR, TEM, XRD) revealed the synthesis of the multifunctional magnetic systems.					
30423746	3	53	dep	magnetite	902:910	arg1	effect					952:957	antioxidant effect	940:957	antioxidant effect	940:957	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	53	dep	magnetite	902:910	arg1	the					898:900	the	898:900	the	898:900	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	6	54	theme	low	1609:1611	arg1	solubility					1613:1622	very low solubility	1604:1622	very low solubility in biological fluids	1604:1643	Moreover, we proposed a nanocarrier that would be able to load unstable active principles and with very low solubility in biological fluids to increase their biological ability.					
30423746	3	55	theme	acid	753:756	arg1	complex					775:781	the protocatechuic acid or its inclusion complex	734:781	the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin	734:825	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	6	56	theme	load	1563:1566	arg1	principles					1584:1593	load unstable active principles	1563:1593	load unstable active principles	1563:1593	Moreover, we proposed a nanocarrier that would be able to load unstable active principles and with very low solubility in biological fluids to increase their biological ability.					
30423746	5	57	theme	biomedical	1480:1489	arg1	applications					1491:1502	biomedical applications	1480:1502	biomedical applications	1480:1502	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	2	58	theme	magnetic	377:384	arg1	nanoparticles					386:398	core-shell magnetic nanoparticles	366:398	core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action	366:499	The purpose of this study was to design, synthesize, and characterize core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action.					
30423746	5	59	theme	system	1272:1277	arg1	suspensions					1290:1300	blood system injectable suspensions	1266:1300	blood system injectable suspensions	1266:1300	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	6	60	theme	active	1577:1582	arg1	principles					1584:1593	load unstable active principles	1563:1593	load unstable active principles	1563:1593	Moreover, we proposed a nanocarrier that would be able to load unstable active principles and with very low solubility in biological fluids to increase their biological ability.					
30423746	1	61	theme	physical	164:171	arg1	properties					173:182	their physical properties	158:182	their physical properties	158:182	Core-shell magnetic nanoparticle synthesis offers the opportunity to engineering their physical properties for specific applications when the intrinsic magnetic properties can be associated with other interesting ones.					
30423746	5	62	theme	normal	1379:1384	arg1	cells					1411:1415	normal human dermal fibroblasts cells	1379:1415	normal human dermal fibroblasts cells	1379:1415	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	1	63	theme	other	272:276	arg1	ones					290:293	other interesting ones	272:293	other interesting ones	272:293	Core-shell magnetic nanoparticle synthesis offers the opportunity to engineering their physical properties for specific applications when the intrinsic magnetic properties can be associated with other interesting ones.					
30423746	3	64	dep	synthesis	539:547	arg1	the					535:537	the	535:537	the	535:537	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	5	65	dep	activity	1315:1322	arg1	using					1325:1329	using	1325:1329	using DPPH test	1325:1339	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	5	66	theme	adequate	1446:1453	arg1	properties					1455:1464	adequate properties	1446:1464	adequate properties	1446:1464	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
30423746	2	67	theme	superoxide	411:420	arg1	dismutase					422:430	superoxide dismutase	411:430	superoxide dismutase activity	411:439	The purpose of this study was to design, synthesize, and characterize core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action.					
30423746	3	68	theme	acid	935:938	arg1	properties					884:893	the useful properties	873:893	the useful properties of the magnetite and the protocatechuic acid antioxidant effect	873:957	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	1	69	theme	interesting	278:288	arg1	ones					290:293	other interesting ones	272:293	other interesting ones	272:293	Core-shell magnetic nanoparticle synthesis offers the opportunity to engineering their physical properties for specific applications when the intrinsic magnetic properties can be associated with other interesting ones.					
30423746	6	70	theme	biological	1627:1636	arg1	fluids					1638:1643	biological fluids	1627:1643	biological fluids	1627:1643	Moreover, we proposed a nanocarrier that would be able to load unstable active principles and with very low solubility in biological fluids to increase their biological ability.					
30423746	3	71	theme	protocatechuic	738:751	arg1	acid					753:756	the protocatechuic acid	734:756	the protocatechuic acid	734:756	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	72	theme	weight	691:696	arg1	polyethyleneimine					656:672	branched polyethyleneimine	647:672	branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery	647:850	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	2	73	theme	dismutase	422:430	arg1	activity					432:439	superoxide dismutase activity	411:439	superoxide dismutase activity	411:439	The purpose of this study was to design, synthesize, and characterize core-shell magnetic nanoparticles that mimic superoxide dismutase activity offering the possibility of guidance and therapeutic action.					
30423746	3	74	theme	antioxidant	940:950	arg1	effect					952:957	antioxidant effect	940:957	antioxidant effect	940:957	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	75	theme	useful	877:882	arg1	properties					884:893	the useful properties	873:893	the useful properties of the magnetite and the protocatechuic acid antioxidant effect	873:957	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	3	76	theme	complex	775:781	arg1	loading					723:729	the loading	719:729	the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery	719:850	We proposed, for the first time, the synthesis and characterization of the nanocarriers comprised of magnetite nanoparticles functionalized with branched polyethyleneimine of low molecular weight (1.8 kDa) permitting the loading of the protocatechuic acid or its inclusion complex with anionic sulfobutylether-β-cyclodextrin for active drug delivery, in order to combine the useful properties of the magnetite and the protocatechuic acid antioxidant effect.					
30423746	1	77	theme	specific	188:195	arg1	applications					197:208	specific applications	188:208	specific applications	188:208	Core-shell magnetic nanoparticle synthesis offers the opportunity to engineering their physical properties for specific applications when the intrinsic magnetic properties can be associated with other interesting ones.					
30423746	5	78	theme	human	1386:1390	arg1	cells					1411:1415	normal human dermal fibroblasts cells	1379:1415	normal human dermal fibroblasts cells	1379:1415	Due to the possibility of being formulated as blood system injectable suspensions, antioxidant activity (using DPPH test) and cytotoxicity (using MTS assay on normal human dermal fibroblasts cells) were also measured, showing adequate properties to be used in biomedical applications.					
31369743	0	0	theme	Potential	100:108	arg1	Candidates					110:119	Potential Candidates	100:119	Potential Candidates for Passive Immunization by Oral Administration	100:167	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	4	1	theme	size	864:867	arg1	terms					810:814	terms	810:814	terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding	810:975	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	4	2	theme	relative	892:899	arg1	solubility					910:919	relative apparent solubility	892:919	relative apparent solubility	892:919	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	2	3	theme	oral	372:375	arg1	delivery					377:384	oral delivery	372:384	oral delivery of ETEC-specific secretory IgAs (sIgAs)	372:424	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	5	4	theme	sample	1038:1043	arg1	heterogeneity					1045:1057	increased sample heterogeneity	1028:1057	increased sample heterogeneity	1028:1057	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	1	5	theme	developing	266:275	arg1	countries					277:285	developing countries	266:285	developing countries	266:285	Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrheal disease among children in developing countries, and there are no licensed vaccines to protect against ETEC.					
31369743	3	6	theme	enterotoxin	718:728	arg1	sIgA2					743:747	sIgA2	743:747	sIgA2	743:747	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	6	theme	enterotoxin	718:728	arg1	IgG1					754:757	IgG1	754:757	IgG1	754:757	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	6	theme	enterotoxin	718:728	arg1	sIgA1					736:740	sIgA1	736:740	sIgA1	736:740	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	6	theme	enterotoxin	718:728	arg1	mAbs					730:733	3 anti-heat-labile enterotoxin mAbs	699:733	3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells)	699:780	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	5	7	theme	N-glycan	1083:1090	arg1	composition					1092:1102	N-glycan composition	1083:1102	N-glycan composition	1083:1102	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	3	8	theme	mAbs	730:733	arg1	attributes					662:671	key structural attributes	647:671	key structural attributes	647:671	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	8	theme	mAbs	730:733	arg1	profiles					687:694	stability profiles	677:694	stability profiles	677:694	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	4	9	theme	aggregate	873:881	arg1	content					883:889	aggregate content	873:889	aggregate content	873:889	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	4	10	theme	structure	827:835	arg1	terms					810:814	terms	810:814	terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding	810:975	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	3	11	theme	techniques	558:567	arg1	series					533:538	a series	531:538	a series of physiochemical techniques and an in vitro gastric digestion model	531:607	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	7	12	theme	stable	1387:1392	arg1	formulations					1404:1415	design stable, low-cost formulations	1380:1415	design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world	1380:1535	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	2	13	from	protection	480:489	arg1	populations					503:513	targeted populations	494:513	targeted populations	494:513	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	6	14	theme	activity	1263:1270	arg1	loss					1239:1242	loss	1239:1242	loss of antigen binding activity	1239:1270	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	4	15	link	N-linked	838:845	arg1	profiles					854:861	N-linked glycan profiles	838:861	N-linked glycan profiles	838:861	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	7	16	theme	sIgA	1420:1423	arg1	mAbs					1425:1428	sIgA mAbs	1420:1428	sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world	1420:1535	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	6	17	theme	low	1293:1295	arg1	37°C					1301:1304	37°C	1301:1304	37°C	1301:1304	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	6	17	theme	low	1293:1295	arg1	pH					1297:1298	low pH	1293:1298	low pH	1293:1298	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	4	18	theme	antigen	961:967	arg1	binding					969:975	in vitro antigen binding	952:975	in vitro antigen binding	952:975	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	5	19	theme	composition	1092:1102	arg1	terms					1074:1078	terms	1074:1078	terms of N-glycan composition	1074:1102	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	5	19	theme	composition	1092:1102	arg1	presence					1112:1119	the presence	1108:1119	the presence of higher molecular weight species	1108:1154	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	4	20	theme	N-linked	838:845	arg1	profiles					854:861	N-linked glycan profiles	838:861	N-linked glycan profiles	838:861	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	7	21	dep	stable	1387:1392	arg1	low-cost					1395:1402	low-cost	1395:1402	low-cost	1395:1402	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	0	22	theme	Passive	125:131	arg1	Immunization					133:144	Passive Immunization	125:144	Passive Immunization by Oral Administration	125:167	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	7	23	theme	mAbs	1425:1428	arg1	formulations					1404:1415	design stable, low-cost formulations	1380:1415	design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world	1380:1535	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	6	24	theme	antigen	1247:1253	arg1	activity					1263:1270	antigen binding activity	1247:1270	antigen binding activity	1247:1270	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	0	25	theme	Preformulation	0:13	arg1	Characterization					15:30	Preformulation Characterization	0:30	Preformulation Characterization	0:30	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	2	26	theme	IgAs	413:416	arg1	delivery					377:384	oral delivery	372:384	oral delivery of ETEC-specific secretory IgAs (sIgAs)	372:424	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	4	27	theme	profiles	854:861	arg1	terms					810:814	terms	810:814	terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding	810:975	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	3	28	theme	gastric	585:591	arg1	model					603:607	an in vitro gastric digestion model	573:607	an in vitro gastric digestion model	573:607	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	7	29	theme	oral	1436:1439	arg1	supplement					1441:1450	an oral supplement	1433:1450	an oral supplement for passive immunization to protect against enteric diseases in the developing world	1433:1535	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	2	30	theme	secretory	403:411	arg1	IgAs					413:416	ETEC-specific secretory IgAs	389:416	ETEC-specific secretory IgAs (sIgAs)	389:424	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	2	30	theme	secretory	403:411	arg1	sIgAs					419:423	sIgAs	419:423	sIgAs	419:423	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	2	31	theme	alternative	455:465	arg1	approach					467:474	an alternative approach	452:474	an alternative approach for protection in targeted populations	452:513	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	6	32	theme	binding	1255:1261	arg1	activity					1263:1270	antigen binding activity	1247:1270	antigen binding activity	1247:1270	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	4	33	theme	glycan	847:852	arg1	profiles					854:861	N-linked glycan profiles	838:861	N-linked glycan profiles	838:861	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	3	34	theme	digestion	593:601	arg1	model					603:607	an in vitro gastric digestion model	573:607	an in vitro gastric digestion model	573:607	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	2	35	theme	ETEC-specific	389:401	arg1	IgAs					413:416	ETEC-specific secretory IgAs	389:416	ETEC-specific secretory IgAs (sIgAs)	389:424	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	2	35	theme	ETEC-specific	389:401	arg1	sIgAs					419:423	sIgAs	419:423	sIgAs	419:423	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	0	36	theme	Stability	36:44	arg1	Assessments					46:56	Stability Assessments	36:56	Stability Assessments	36:56	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	3	37	theme	CHO	771:773	arg1	cells					775:779	CHO cells	771:779	CHO cells	771:779	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	0	38	theme	Oral	149:152	arg1	Administration					154:167	Oral Administration	149:167	Oral Administration	149:167	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	4	39	theme	primary	819:825	arg1	structure					827:835	primary structure	819:835	primary structure	819:835	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	1	40	theme	major	215:219	arg1	cause					221:225	a major cause	213:225	a major cause of diarrheal disease among children in developing countries	213:285	Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrheal disease among children in developing countries, and there are no licensed vaccines to protect against ETEC.					
31369743	1	40	theme	major	215:219	arg1	coli					198:201	Escherichia coli	186:201	Enterotoxigenic Escherichia coli (ETEC)	170:208	Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrheal disease among children in developing countries, and there are no licensed vaccines to protect against ETEC.					
31369743	6	41	from	pH	1297:1298	arg1	incubation					1279:1288	incubation	1279:1288	incubation at low pH, 37°C with pepsin	1279:1316	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	4	42	theme	solubility	910:919	arg1	terms					810:814	terms	810:814	terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding	810:975	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	6	43	theme	overall	1178:1184	arg1	stability					1202:1210	overall better physical stability	1178:1210	overall better physical stability	1178:1210	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	3	44	theme	in vitro	576:583	arg1	model					603:607	an in vitro gastric digestion model	573:607	an in vitro gastric digestion model	573:607	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	6	45	theme	physical	1193:1200	arg1	stability					1202:1210	overall better physical stability	1178:1210	overall better physical stability	1178:1210	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	2	46	theme	targeted	494:501	arg1	populations					503:513	targeted populations	494:513	targeted populations	494:513	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	1	47	theme	licensed	305:312	arg1	vaccines					314:321	no licensed vaccines	302:321	no licensed vaccines	302:321	Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrheal disease among children in developing countries, and there are no licensed vaccines to protect against ETEC.					
31369743	4	48	theme	stability	937:945	arg1	terms					810:814	terms	810:814	terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding	810:975	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	3	49	theme	stability	677:685	arg1	profiles					687:694	stability profiles	677:694	stability profiles	677:694	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	7	50	theme	future	1359:1364	arg1	challenges					1366:1375	future challenges	1359:1375	future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world	1359:1535	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	7	51	theme	enteric	1496:1502	arg1	diseases					1504:1511	enteric diseases	1496:1511	enteric diseases in the developing world	1496:1535	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	7	52	from	diseases	1504:1511	arg1	world					1531:1535	the developing world	1516:1535	the developing world	1516:1535	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	5	53	theme	weight	1141:1146	arg1	species					1148:1154	higher molecular weight species	1124:1154	higher molecular weight species	1124:1154	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	4	54	theme	conformational	922:935	arg1	stability					937:945	conformational stability	922:945	conformational stability	922:945	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	3	55	theme	structural	651:660	arg1	attributes					662:671	key structural attributes	647:671	key structural attributes	647:671	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	5	56	theme	sIgA2	1010:1014	arg1	mAbs					1016:1019	sIgA1 and sIgA2 mAbs	1000:1019	sIgA1 and sIgA2 mAbs	1000:1019	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	1	57	theme	diarrheal	230:238	arg1	disease					240:246	diarrheal disease	230:246	diarrheal disease among children in developing countries	230:285	Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrheal disease among children in developing countries, and there are no licensed vaccines to protect against ETEC.					
31369743	0	58	theme	IgA	71:73	arg1	Antibodies					86:95	Secretory IgA Monoclonal Antibodies	61:95	Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration	61:167	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	5	59	theme	species	1148:1154	arg1	terms					1074:1078	terms	1074:1078	terms of N-glycan composition	1074:1102	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	5	59	theme	species	1148:1154	arg1	presence					1112:1119	the presence	1108:1119	the presence of higher molecular weight species	1108:1154	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	1	60	from	children	254:261	arg1	countries					277:285	developing countries	266:285	developing countries	266:285	Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrheal disease among children in developing countries, and there are no licensed vaccines to protect against ETEC.					
31369743	3	61	dep	mAbs	730:733	arg1	sIgA2					743:747	sIgA2	743:747	sIgA2	743:747	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	61	dep	mAbs	730:733	arg1	IgG1					754:757	IgG1	754:757	IgG1	754:757	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	61	dep	mAbs	730:733	arg1	sIgA1					736:740	sIgA1	736:740	sIgA1	736:740	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	61	dep	mAbs	730:733	arg1	mAbs					730:733	3 anti-heat-labile enterotoxin mAbs	699:733	3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells)	699:780	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	62	theme	model	603:607	arg1	series					533:538	a series	531:538	a series of physiochemical techniques and an in vitro gastric digestion model	531:607	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	5	63	theme	higher	1124:1129	arg1	species					1148:1154	higher molecular weight species	1124:1154	higher molecular weight species	1124:1154	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	0	64	theme	Secretory	61:69	arg1	Antibodies					86:95	Secretory IgA Monoclonal Antibodies	61:95	Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration	61:167	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	5	65	theme	increased	1028:1036	arg1	heterogeneity					1045:1057	increased sample heterogeneity	1028:1057	increased sample heterogeneity	1028:1057	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	4	66	theme	binding	969:975	arg1	terms					810:814	terms	810:814	terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding	810:975	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	2	67	theme	Passive	348:354	arg1	immunization					356:367	Passive immunization	348:367	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs)	348:424	Passive immunization by oral delivery of ETEC-specific secretory IgAs (sIgAs) could potentially provide an alternative approach for protection in targeted populations.					
31369743	7	68	theme	developing	1520:1529	arg1	world					1531:1535	the developing world	1516:1535	the developing world	1516:1535	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	4	69	theme	apparent	901:908	arg1	solubility					910:919	relative apparent solubility	892:919	relative apparent solubility	892:919	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
31369743	3	70	theme	anti-heat-labile	701:716	arg1	sIgA2					743:747	sIgA2	743:747	sIgA2	743:747	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	70	theme	anti-heat-labile	701:716	arg1	IgG1					754:757	IgG1	754:757	IgG1	754:757	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	70	theme	anti-heat-labile	701:716	arg1	sIgA1					736:740	sIgA1	736:740	sIgA1	736:740	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	70	theme	anti-heat-labile	701:716	arg1	mAbs					730:733	3 anti-heat-labile enterotoxin mAbs	699:733	3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells)	699:780	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	5	71	theme	IgG1	990:993	arg1	mAb					995:997	IgG1 mAb	990:997	IgG1 mAb	990:997	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	7	72	theme	challenges	1366:1375	arg1	terms					1350:1354	terms	1350:1354	terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world	1350:1535	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	1	73	theme	disease	240:246	arg1	cause					221:225	a major cause	213:225	a major cause of diarrheal disease among children in developing countries	213:285	Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrheal disease among children in developing countries, and there are no licensed vaccines to protect against ETEC.					
31369743	1	73	theme	disease	240:246	arg1	coli					198:201	Escherichia coli	186:201	Enterotoxigenic Escherichia coli (ETEC)	170:208	Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrheal disease among children in developing countries, and there are no licensed vaccines to protect against ETEC.					
31369743	3	74	theme	physiochemical	543:556	arg1	techniques					558:567	physiochemical techniques	543:567	physiochemical techniques	543:567	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	75	theme	key	647:649	arg1	attributes					662:671	key structural attributes	647:671	key structural attributes	647:671	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	3	76	used	used	614:617	arg2	series					533:538	a series	531:538	a series of physiochemical techniques and an in vitro gastric digestion model	531:607	In this study, a series of physiochemical techniques and an in vitro gastric digestion model were used to characterize and compare key structural attributes and stability profiles of 3 anti-heat-labile enterotoxin mAbs (sIgA1, sIgA2, and IgG1 produced in CHO cells).					
31369743	0	77	theme	Antibodies	86:95	arg1	Characterization					15:30	Preformulation Characterization	0:30	Preformulation Characterization	0:30	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	0	77	theme	Antibodies	86:95	arg1	Assessments					46:56	Stability Assessments	36:56	Stability Assessments	36:56	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	5	78	theme	molecular	1131:1139	arg1	species					1148:1154	higher molecular weight species	1124:1154	higher molecular weight species	1124:1154	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	6	79	theme	sIgA	1161:1164	arg1	mAbs					1166:1169	The sIgA mAbs	1157:1169	The sIgA mAbs	1157:1169	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	6	79	theme	sIgA	1161:1164	arg1	resistant					1226:1234	resistant	1226:1234	resistant	1226:1234	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	6	80	with	incubation	1279:1288	arg1	pepsin					1311:1316	pepsin	1311:1316	pepsin	1311:1316	The sIgA mAbs showed overall better physical stability and were more resistant to loss of antigen binding activity during incubation at low pH, 37°C with pepsin.					
31369743	0	81	theme	Monoclonal	75:84	arg1	Antibodies					86:95	Secretory IgA Monoclonal Antibodies	61:95	Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration	61:167	Preformulation Characterization and Stability Assessments of Secretory IgA Monoclonal Antibodies as Potential Candidates for Passive Immunization by Oral Administration.					
31369743	7	82	theme	passive	1456:1462	arg1	immunization					1464:1475	passive immunization	1456:1475	passive immunization	1456:1475	These results are discussed in terms of future challenges to design stable, low-cost formulations of sIgA mAbs as an oral supplement for passive immunization to protect against enteric diseases in the developing world.					
31369743	5	83	theme	sIgA1	1000:1004	arg1	mAbs					1016:1019	sIgA1 and sIgA2 mAbs	1000:1019	sIgA1 and sIgA2 mAbs	1000:1019	Compared to IgG1 mAb, sIgA1 and sIgA2 mAbs showed increased sample heterogeneity, especially in terms of N-glycan composition and the presence of higher molecular weight species.					
31369743	4	84	theme	in vitro	952:959	arg1	binding					969:975	in vitro antigen binding	952:975	in vitro antigen binding	952:975	The mAbs were evaluated in terms of primary structure, N-linked glycan profiles, size and aggregate content, relative apparent solubility, conformational stability, and in vitro antigen binding.					
30655577	6	0	from	clustering	1118:1127	arg1	mice					1197:1200	male and female mice	1181:1200	male and female mice	1181:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	6	1	from	mice	1197:1200	arg1	clustering					1118:1127	distinct clustering	1109:1127	distinct clustering of physiological, neural, and behavioral effects in male and female mice	1109:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	2	2	theme	intestinal	368:377	arg1	health					379:384	intestinal health	368:384	intestinal health	368:384	As both gut microbiota and intestinal health can influence social and anxiety-like behaviors, we investigated whether emulsifier consumption would detrimentally influence behavior.					
30655577	6	3	from	microbiota	1288:1297	arg1	syndrome					1251:1258	a syndrome	1249:1258	a syndrome of sex-dependent changes in microbiota, physiology, and behavior	1249:1323	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	1	4	theme	chronic	253:259	arg1	inflammation					271:282	chronic low-grade inflammation	253:282	chronic low-grade inflammation	253:282	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	3	5	theme	emulsifier	746:755	arg1	consumption					757:767	emulsifier consumption	746:767	emulsifier consumption	746:767	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	6	6	from	effects	1170:1176	arg1	mice					1197:1200	male and female mice	1181:1200	male and female mice	1181:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	5	7	theme	feeding	995:1001	arg1	modulation					981:990	the modulation	977:990	the modulation of feeding	977:1001	It also changed expression of neuropeptides implicated in the modulation of feeding as well as social and anxiety-related behaviors.					
30655577	1	8	theme	low-grade	261:269	arg1	inflammation					271:282	chronic low-grade inflammation	253:282	chronic low-grade inflammation	253:282	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	6	9	theme	female	1190:1195	arg1	mice					1197:1200	male and female mice	1181:1200	male and female mice	1181:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	7	10	theme	social	1439:1444	arg1	behaviors					1446:1454	anxiety-related and social behaviors	1419:1454	anxiety-related and social behaviors	1419:1454	This study reveals that these commonly used food additives may potentially negatively impact anxiety-related and social behaviors and may do so via different mechanisms in males and females.					
30655577	1	11	from	dysregulations	317:330	arg1	mice					335:338	mice	335:338	mice	335:338	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	4	12	from	behaviors	860:868	arg1	males					873:877	males	873:877	males	873:877	Importantly, emulsifier treatment altered anxiety-like behaviors in males and reduced social behavior in females.					
30655577	3	13	theme	increased	601:609	arg1	adiposity					611:619	increased adiposity	601:619	increased adiposity	601:619	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	3	14	from	adiposity	611:619	arg1	mice					685:688	both male and female mice	664:688	both male and female mice	664:688	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	0	15	theme	sex-dependent	94:106	arg1	manner					108:113	a sex-dependent manner	92:113	a sex-dependent manner	92:113	Dietary emulsifiers consumption alters anxiety-like and social-related behaviors in mice in a sex-dependent manner.					
30655577	6	16	theme	changes	1277:1283	arg1	syndrome					1251:1258	a syndrome	1249:1258	a syndrome of sex-dependent changes in microbiota, physiology, and behavior	1249:1323	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	7	17	theme	used	1365:1368	arg1	additives					1375:1383	these commonly used food additives	1350:1383	these commonly used food additives	1350:1383	This study reveals that these commonly used food additives may potentially negatively impact anxiety-related and social behaviors and may do so via different mechanisms in males and females.					
30655577	7	18	theme	food	1370:1373	arg1	additives					1375:1383	these commonly used food additives	1350:1383	these commonly used food additives	1350:1383	This study reveals that these commonly used food additives may potentially negatively impact anxiety-related and social behaviors and may do so via different mechanisms in males and females.					
30655577	6	19	theme	male	1181:1184	arg1	mice					1197:1200	male and female mice	1181:1200	male and female mice	1181:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	6	20	theme	physiological	1132:1144	arg1	effects					1170:1176	physiological, neural, and behavioral effects	1132:1176	physiological, neural, and behavioral effects in male and female mice	1132:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	6	21	theme	effects	1170:1176	arg1	clustering					1118:1127	distinct clustering	1109:1127	distinct clustering of physiological, neural, and behavioral effects in male and female mice	1109:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	2	22	theme	anxiety-like	411:422	arg1	behaviors					424:432	social and anxiety-like behaviors	400:432	behaviors	424:432	As both gut microbiota and intestinal health can influence social and anxiety-like behaviors, we investigated whether emulsifier consumption would detrimentally influence behavior.					
30655577	7	23	from	mechanisms	1484:1493	arg1	females					1508:1514	females	1508:1514	females	1508:1514	This study reveals that these commonly used food additives may potentially negatively impact anxiety-related and social behaviors and may do so via different mechanisms in males and females.					
30655577	7	23	from	mechanisms	1484:1493	arg1	males					1498:1502	males	1498:1502	males	1498:1502	This study reveals that these commonly used food additives may potentially negatively impact anxiety-related and social behaviors and may do so via different mechanisms in males and females.					
30655577	5	24	theme	neuropeptides	949:961	arg1	expression					935:944	expression	935:944	expression of neuropeptides implicated in the modulation of feeding as well as social and anxiety-related behaviors	935:1049	It also changed expression of neuropeptides implicated in the modulation of feeding as well as social and anxiety-related behaviors.					
30655577	0	25	theme	emulsifiers	8:18	arg1	consumption					20:30	Dietary emulsifiers consumption	0:30	Dietary emulsifiers consumption	0:30	Dietary emulsifiers consumption alters anxiety-like and social-related behaviors in mice in a sex-dependent manner.					
30655577	3	26	theme	sex-dependent	783:795	arg1	manner					797:802	a sex-dependent manner	781:802	a sex-dependent manner	781:802	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	3	27	theme	altered	626:632	arg1	composition					649:659	altered gut microbiota composition	626:659	altered gut microbiota composition	626:659	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	6	28	from	changes	1277:1283	arg1	physiology					1300:1309	physiology	1300:1309	physiology	1300:1309	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	6	28	from	changes	1277:1283	arg1	behavior					1316:1323	behavior	1316:1323	behavior	1316:1323	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	6	28	from	changes	1277:1283	arg1	microbiota					1288:1297	microbiota	1288:1297	microbiota	1288:1297	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	2	29	theme	social	400:405	arg1	behaviors					424:432	social and anxiety-like behaviors	400:432	behaviors	424:432	As both gut microbiota and intestinal health can influence social and anxiety-like behaviors, we investigated whether emulsifier consumption would detrimentally influence behavior.					
30655577	0	30	theme	Dietary	0:6	arg1	consumption					20:30	Dietary emulsifiers consumption	0:30	Dietary emulsifiers consumption	0:30	Dietary emulsifiers consumption alters anxiety-like and social-related behaviors in mice in a sex-dependent manner.					
30655577	3	31	theme	gut	634:636	arg1	composition					649:659	altered gut microbiota composition	626:659	altered gut microbiota composition	626:659	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	6	32	theme	behavioral	1159:1168	arg1	effects					1170:1176	physiological, neural, and behavioral effects	1132:1176	physiological, neural, and behavioral effects in male and female mice	1132:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	2	33	theme	emulsifier	459:468	arg1	consumption					470:480	emulsifier consumption	459:480	emulsifier consumption	459:480	As both gut microbiota and intestinal health can influence social and anxiety-like behaviors, we investigated whether emulsifier consumption would detrimentally influence behavior.					
30655577	4	34	theme	social	891:896	arg1	behavior					898:905	social behavior	891:905	social behavior in females	891:916	Importantly, emulsifier treatment altered anxiety-like behaviors in males and reduced social behavior in females.					
30655577	3	35	from	composition	649:659	arg1	mice					685:688	both male and female mice	664:688	both male and female mice	664:688	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	5	36	theme	anxiety-related	1025:1039	arg1	behaviors					1041:1049	anxiety-related behaviors	1025:1049	anxiety-related behaviors	1025:1049	It also changed expression of neuropeptides implicated in the modulation of feeding as well as social and anxiety-related behaviors.					
30655577	6	37	theme	sex-dependent	1263:1275	arg1	changes					1277:1283	sex-dependent changes	1263:1283	sex-dependent changes in microbiota, physiology, and behavior	1263:1323	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	1	38	theme	Dietary	116:122	arg1	CMC					161:163	CMC	161:163	CMC	161:163	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	1	38	theme	Dietary	116:122	arg1	carboxylmethylcellulose					136:158	Dietary emulsifiers carboxylmethylcellulose	116:158	Dietary emulsifiers carboxylmethylcellulose (CMC)	116:164	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	6	39	from	syndrome	1251:1258	arg1	physiology					1300:1309	physiology	1300:1309	physiology	1300:1309	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	6	39	from	syndrome	1251:1258	arg1	behavior					1316:1323	behavior	1316:1323	behavior	1316:1323	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	6	39	from	syndrome	1251:1258	arg1	microbiota					1288:1297	microbiota	1288:1297	microbiota	1288:1297	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	1	40	theme	metabolic	307:315	arg1	dysregulations					317:330	metabolic dysregulations	307:330	metabolic dysregulations in mice	307:338	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	3	41	theme	specific	704:711	arg1	taxa					723:726	the specific microboal taxa	700:726	the specific microboal taxa altered following emulsifier consumption	700:767	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	6	42	theme	Multivariate	1052:1063	arg1	analyses					1065:1072	Multivariate analyses	1052:1072	Multivariate analyses	1052:1072	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	6	43	theme	neural	1147:1152	arg1	effects					1170:1176	physiological, neural, and behavioral effects	1132:1176	physiological, neural, and behavioral effects in male and female mice	1132:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	0	44	theme	anxiety-like	39:50	arg1	behaviors					71:79	anxiety-like and social-related behaviors	39:79	anxiety-like and social-related behaviors	39:79	Dietary emulsifiers consumption alters anxiety-like and social-related behaviors in mice in a sex-dependent manner.					
30655577	3	45	theme	microbiota	638:647	arg1	composition					649:659	altered gut microbiota composition	626:659	altered gut microbiota composition	626:659	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	6	46	theme	emulsifier	1219:1228	arg1	treatment					1230:1238	emulsifier treatment	1219:1238	emulsifier treatment	1219:1238	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	1	47	theme	emulsifiers	124:134	arg1	CMC					161:163	CMC	161:163	CMC	161:163	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	1	47	theme	emulsifiers	124:134	arg1	carboxylmethylcellulose					136:158	Dietary emulsifiers carboxylmethylcellulose	116:158	Dietary emulsifiers carboxylmethylcellulose (CMC)	116:164	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	0	48	theme	social-related	56:69	arg1	behaviors					71:79	anxiety-like and social-related behaviors	39:79	anxiety-like and social-related behaviors	39:79	Dietary emulsifiers consumption alters anxiety-like and social-related behaviors in mice in a sex-dependent manner.					
30655577	7	49	theme	different	1474:1482	arg1	mechanisms					1484:1493	different mechanisms	1474:1493	different mechanisms in males and females	1474:1514	This study reveals that these commonly used food additives may potentially negatively impact anxiety-related and social behaviors and may do so via different mechanisms in males and females.					
30655577	2	50	theme	gut	349:351	arg1	microbiota					353:362	gut microbiota	349:362	gut microbiota	349:362	As both gut microbiota and intestinal health can influence social and anxiety-like behaviors, we investigated whether emulsifier consumption would detrimentally influence behavior.					
30655577	6	51	from	behavior	1316:1323	arg1	syndrome					1251:1258	a syndrome	1249:1258	a syndrome of sex-dependent changes in microbiota, physiology, and behavior	1249:1323	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	3	52	theme	female	678:683	arg1	mice					685:688	both male and female mice	664:688	both male and female mice	664:688	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	4	53	from	behavior	898:905	arg1	females					910:916	females	910:916	females	910:916	Importantly, emulsifier treatment altered anxiety-like behaviors in males and reduced social behavior in females.					
30655577	1	54	theme	intestinal	220:229	arg1	microbiota					231:240	the intestinal microbiota	216:240	the intestinal microbiota	216:240	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	3	55	theme	microboal	713:721	arg1	taxa					723:726	the specific microboal taxa	700:726	the specific microboal taxa altered following emulsifier consumption	700:767	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	4	56	theme	anxiety-like	847:858	arg1	behaviors					860:868	anxiety-like behaviors	847:868	anxiety-like behaviors in males	847:877	Importantly, emulsifier treatment altered anxiety-like behaviors in males and reduced social behavior in females.					
30655577	4	57	theme	emulsifier	818:827	arg1	treatment					829:837	emulsifier treatment	818:837	emulsifier treatment	818:837	Importantly, emulsifier treatment altered anxiety-like behaviors in males and reduced social behavior in females.					
30655577	5	58	theme	social	1014:1019	arg1	expression					935:944	expression	935:944	expression of neuropeptides implicated in the modulation of feeding as well as social and anxiety-related behaviors	935:1049	It also changed expression of neuropeptides implicated in the modulation of feeding as well as social and anxiety-related behaviors.					
30655577	7	59	theme	anxiety-related	1419:1433	arg1	behaviors					1446:1454	anxiety-related and social behaviors	1419:1454	anxiety-related and social behaviors	1419:1454	This study reveals that these commonly used food additives may potentially negatively impact anxiety-related and social behaviors and may do so via different mechanisms in males and females.					
30655577	1	60	theme	microbiota	231:240	arg1	composition					201:211	the composition	197:211	the composition of the intestinal microbiota	197:240	Dietary emulsifiers carboxylmethylcellulose (CMC) and polysorbate 80 (P80) alter the composition of the intestinal microbiota and induce chronic low-grade inflammation, ultimately leading to metabolic dysregulations in mice.					
30655577	6	61	from	physiology	1300:1309	arg1	syndrome					1251:1258	a syndrome	1249:1258	a syndrome of sex-dependent changes in microbiota, physiology, and behavior	1249:1323	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
30655577	3	62	theme	male	669:672	arg1	mice					685:688	both male and female mice	664:688	both male and female mice	664:688	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	3	63	from	inflammation	587:598	arg1	mice					685:688	both male and female mice	664:688	both male and female mice	664:688	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	3	64	theme	chronic	568:574	arg1	inflammation					587:598	chronic intestinal inflammation	568:598	chronic intestinal inflammation	568:598	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	3	65	theme	intestinal	576:585	arg1	inflammation					587:598	chronic intestinal inflammation	568:598	chronic intestinal inflammation	568:598	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	3	66	theme	emulsifier	540:549	arg1	exposure					551:558	emulsifier exposure	540:558	emulsifier exposure	540:558	We confirmed that emulsifier exposure induced chronic intestinal inflammation, increased adiposity, and altered gut microbiota composition in both male and female mice, although the specific microboal taxa altered following emulsifier consumption occurred in a sex-dependent manner.					
30655577	6	67	theme	distinct	1109:1116	arg1	clustering					1118:1127	distinct clustering	1109:1127	distinct clustering of physiological, neural, and behavioral effects in male and female mice	1109:1200	Multivariate analyses revealed that CMC and P80 produced distinct clustering of physiological, neural, and behavioral effects in male and female mice, suggesting that emulsifier treatment leads to a syndrome of sex-dependent changes in microbiota, physiology, and behavior.					
29423317	9	0	theme	vaccines	1641:1648	arg1	production					1602:1611	the production	1598:1611	the production of S. Paratyphi A conjugate vaccines	1598:1648	Our system provides an easier and more economical method for the production of S. Paratyphi A conjugate vaccines.					
29423317	0	1	link	O-linked	76:83	arg1	system					99:104	an O-linked glycosylation system	73:104	an O-linked glycosylation system in vivo	73:112	Design and production of conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo.					
29423317	3	2	theme	Paratyphi	690:698	arg1	vaccine					712:718	an S. Paratyphi A conjugate vaccine	684:718	an S. Paratyphi A conjugate vaccine	684:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	10	3	theme	precise	1767:1773	arg1	vaccines					1785:1792	next-generation "precise conjugate vaccines	1750:1792	next-generation "precise conjugate vaccines	1750:1792	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	6	4	theme	other	1183:1187	arg1	strains					1198:1204	five other epidemic strains	1178:1204	five other epidemic strains	1178:1204	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	10	5	theme	next-generation	1750:1764	arg1	vaccines					1785:1792	next-generation "precise conjugate vaccines	1750:1792	next-generation "precise conjugate vaccines	1750:1792	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	1	6	theme	Paratyphi	175:183	arg1	A					185:185	Salmonella enterica serovar Paratyphi A	147:185	Salmonella enterica serovar Paratyphi A	147:185	Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide.					
29423317	6	7	theme	CMCC	1163:1166	arg1	50973					1168:1172	A strain CMCC 50973	1154:1172	S. Paratyphi A strain CMCC 50973	1141:1172	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	10	8	dep	provides	1697:1704	arg1	"					1794:1794	"	1794:1794	"	1794:1794	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	10	9	theme	vaccines	1785:1792	arg1	development					1735:1745	the development	1731:1745	the development of next-generation "precise conjugate vaccines	1731:1792	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	8	10	theme	animal	1441:1446	arg1	challenge					1448:1456	animal challenge	1441:1456	animal challenge	1441:1456	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	3	11	theme	glycosylation	597:609	arg1	system					664:669	the only currently available glycosylation system	621:669	the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	621:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	3	11	theme	glycosylation	597:609	arg1	approach					611:618	an O-linked glycosylation approach	585:618	an O-linked glycosylation approach	585:618	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	4	12	theme	Paratyphi	764:772	arg1	strain					776:781	a recombinant S. Paratyphi A strain	747:781	a recombinant S. Paratyphi A strain	747:781	We successfully generated a recombinant S. Paratyphi A strain with a longer O-polysaccharide chain and transformed the O-linked glycosylation system into the strain.					
29423317	5	13	theme	O-polysaccharide	936:951	arg1	vector					964:969	an O-polysaccharide expression vector	933:969	an O-polysaccharide expression vector	933:969	Thus, we avoided the need for construction of an O-polysaccharide expression vector.					
29423317	8	14	theme	potential	1490:1498	arg1	strategy					1500:1507	a potential strategy	1488:1507	a potential strategy for further vaccine design	1488:1534	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	0	15	theme	O-linked	76:83	arg1	system					99:104	an O-linked glycosylation system	73:104	an O-linked glycosylation system in vivo	73:112	Design and production of conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo.					
29423317	8	16	theme	vaccine	1521:1527	arg1	design					1529:1534	further vaccine design	1513:1534	further vaccine design	1513:1534	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	10	17	theme	site	1685:1688	arg1	sequon					1690:1695	the glycosylation site sequon	1667:1695	the glycosylation site sequon	1667:1695	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	8	18	theme	anti-lipopolysaccharide	1340:1362	arg1	titer					1373:1377	the anti-lipopolysaccharide antibody titer	1336:1377	the anti-lipopolysaccharide antibody titer	1336:1377	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	9	19	theme	easier	1560:1565	arg1	method					1587:1592	an easier and more economical method	1557:1592	an easier and more economical method for the production of S. Paratyphi A conjugate vaccines	1557:1648	Our system provides an easier and more economical method for the production of S. Paratyphi A conjugate vaccines.					
29423317	6	20	theme	CMCC	1089:1092	arg1	50973					1094:1098	A strain CMCC 50973	1080:1098	A strain CMCC 50973	1080:1098	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	4	21	theme	recombinant	749:759	arg1	strain					776:781	a recombinant S. Paratyphi A strain	747:781	a recombinant S. Paratyphi A strain	747:781	We successfully generated a recombinant S. Paratyphi A strain with a longer O-polysaccharide chain and transformed the O-linked glycosylation system into the strain.					
29423317	5	22	theme	vector	964:969	arg1	construction					917:928	construction	917:928	construction of an O-polysaccharide expression vector	917:969	Thus, we avoided the need for construction of an O-polysaccharide expression vector.					
29423317	8	23	theme	bactericidal	1380:1391	arg1	activity					1393:1400	bactericidal activity	1380:1400	bactericidal activity of serum	1380:1409	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	10	24	theme	sequon	1690:1695	arg1	Modification					1651:1662	Modification	1651:1662	Modification of the glycosylation site sequon	1651:1695	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	2	25	theme	bacterial	385:393	arg1	infection					395:403	bacterial infection	385:403	bacterial infection	385:403	As yet, there are no licensed vaccines against S. Paratyphi A. Biosynthesis of conjugate vaccines has become a promising approach against bacterial infection.					
29423317	3	26	theme	biosynthetic	427:438	arg1	strategy					440:447	the popular biosynthetic strategy	415:447	the popular biosynthetic strategy using N-linked glycosylation systems	415:484	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	0	27	theme	conjugate	25:33	arg1	vaccines					35:42	conjugate vaccines	25:42	conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo	25:112	Design and production of conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo.					
29423317	3	28	link	O-linked	588:595	arg1	system					664:669	the only currently available glycosylation system	621:669	the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	621:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	3	28	link	O-linked	588:595	arg1	approach					611:618	an O-linked glycosylation approach	585:618	an O-linked glycosylation approach	585:618	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	3	29	theme	available	640:648	arg1	system					664:669	the only currently available glycosylation system	621:669	the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	621:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	3	29	theme	available	640:648	arg1	approach					611:618	an O-linked glycosylation approach	585:618	an O-linked glycosylation approach	585:618	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	4	30	theme	glycosylation	849:861	arg1	system					863:868	the O-linked glycosylation system	836:868	the O-linked glycosylation system	836:868	We successfully generated a recombinant S. Paratyphi A strain with a longer O-polysaccharide chain and transformed the O-linked glycosylation system into the strain.					
29423317	8	31	theme	protective	1416:1425	arg1	effect					1427:1432	protective effect	1416:1432	protective effect during animal challenge	1416:1456	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	3	32	theme	glycosylation	464:476	arg1	systems					478:484	N-linked glycosylation systems	455:484	N-linked glycosylation systems	455:484	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	1	33	theme	common	198:203	arg1	worldwide					236:244	a common and serious infectious disease worldwide	196:244	a common and serious infectious disease worldwide	196:244	Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide.					
29423317	9	34	theme	Paratyphi	1619:1627	arg1	vaccines					1641:1648	S. Paratyphi A conjugate vaccines	1616:1648	S. Paratyphi A conjugate vaccines	1616:1648	Our system provides an easier and more economical method for the production of S. Paratyphi A conjugate vaccines.					
29423317	3	35	theme	specialized	509:519	arg1	structure					538:546	the specialized O-polysaccharide structure	505:546	the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	505:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	1	36	theme	serious	209:215	arg1	worldwide					236:244	a common and serious infectious disease worldwide	196:244	a common and serious infectious disease worldwide	196:244	Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide.					
29423317	9	37	theme	conjugate	1631:1639	arg1	vaccines					1641:1648	S. Paratyphi A conjugate vaccines	1616:1648	S. Paratyphi A conjugate vaccines	1616:1648	Our system provides an easier and more economical method for the production of S. Paratyphi A conjugate vaccines.					
29423317	6	38	theme	bactericidal	1111:1122	arg1	activity					1124:1131	bactericidal activity	1111:1131	bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains	1111:1204	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	2	39	theme	conjugate	326:334	arg1	vaccines					336:343	conjugate vaccines	326:343	conjugate vaccines	326:343	As yet, there are no licensed vaccines against S. Paratyphi A. Biosynthesis of conjugate vaccines has become a promising approach against bacterial infection.					
29423317	1	40	theme	disease	228:234	arg1	worldwide					236:244	a common and serious infectious disease worldwide	196:244	a common and serious infectious disease worldwide	196:244	Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide.					
29423317	7	41	theme	glycosylation	1255:1267	arg1	site					1269:1272	the glycosylation site	1251:1272	the glycosylation site (Ser)	1251:1278	Furthermore, we replaced the peptides after the glycosylation site (Ser) with an antigenic peptide (P2).					
29423317	7	41	theme	glycosylation	1255:1267	arg1	Ser					1275:1277	Ser	1275:1277	Ser	1275:1277	Furthermore, we replaced the peptides after the glycosylation site (Ser) with an antigenic peptide (P2).					
29423317	3	42	theme	A	700:700	arg1	vaccine					712:718	an S. Paratyphi A conjugate vaccine	684:718	an S. Paratyphi A conjugate vaccine	684:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	1	43	dep	Salmonella	147:156	arg1	enterica					158:165	enterica	158:165	enterica	158:165	Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide.					
29423317	10	44	theme	conjugate	1775:1783	arg1	vaccines					1785:1792	next-generation "precise conjugate vaccines	1750:1792	next-generation "precise conjugate vaccines	1750:1792	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	3	45	link	N-linked	455:462	arg1	systems					478:484	N-linked glycosylation systems	455:484	N-linked glycosylation systems	455:484	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	6	46	theme	epidemic	1189:1196	arg1	strains					1198:1204	five other epidemic strains	1178:1204	five other epidemic strains	1178:1204	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	1	47	theme	Salmonella	147:156	arg1	A					185:185	Salmonella enterica serovar Paratyphi A	147:185	Salmonella enterica serovar Paratyphi A	147:185	Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide.					
29423317	10	48	theme	glycosylation	1671:1683	arg1	sequon					1690:1695	the glycosylation site sequon	1667:1695	the glycosylation site sequon	1667:1695	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	1	49	theme	serovar	167:173	arg1	A					185:185	Salmonella enterica serovar Paratyphi A	147:185	Salmonella enterica serovar Paratyphi A	147:185	Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide.					
29423317	4	50	theme	O-linked	840:847	arg1	system					863:868	the O-linked glycosylation system	836:868	the O-linked glycosylation system	836:868	We successfully generated a recombinant S. Paratyphi A strain with a longer O-polysaccharide chain and transformed the O-linked glycosylation system into the strain.					
29423317	2	51	theme	licensed	268:275	arg1	vaccines					277:284	no licensed vaccines	265:284	no licensed vaccines against S. Paratyphi A. Biosynthesis of conjugate vaccines has become a promising approach against bacterial infection	265:403	As yet, there are no licensed vaccines against S. Paratyphi A. Biosynthesis of conjugate vaccines has become a promising approach against bacterial infection.					
29423317	6	52	theme	strain	1156:1161	arg1	50973					1168:1172	A strain CMCC 50973	1154:1172	S. Paratyphi A strain CMCC 50973	1141:1172	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	3	53	theme	O-linked	588:595	arg1	system					664:669	the only currently available glycosylation system	621:669	the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	621:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	3	53	theme	O-linked	588:595	arg1	approach					611:618	an O-linked glycosylation approach	585:618	an O-linked glycosylation approach	585:618	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	6	54	theme	In	972:973	arg1	assays					980:985	In vivo assays	972:985	In vivo assays	972:985	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	6	55	dep	In	972:973	arg1	vivo					975:978	vivo	975:978	vivo	975:978	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	10	56	gly	glycosylation	1671:1683	arg2	sequon					1690:1695	the glycosylation site sequon	1667:1695	the glycosylation site sequon	1667:1695	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	10	56	gly	glycosylation	1671:1683	arg2	site					1685:1688	the glycosylation site sequon	1667:1695	the glycosylation site sequon	1667:1695	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	4	57	theme	O-polysaccharide	797:812	arg1	chain					814:818	a longer O-polysaccharide chain	788:818	a longer O-polysaccharide chain	788:818	We successfully generated a recombinant S. Paratyphi A strain with a longer O-polysaccharide chain and transformed the O-linked glycosylation system into the strain.					
29423317	1	58	theme	Enteric	115:121	arg1	fever					123:127	Enteric fever	115:127	Enteric fever	115:127	Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide.					
29423317	3	59	theme	A.	564:565	arg1	structure					538:546	the specialized O-polysaccharide structure	505:546	the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	505:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	3	60	theme	popular	419:425	arg1	strategy					440:447	the popular biosynthetic strategy	415:447	the popular biosynthetic strategy using N-linked glycosylation systems	415:484	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	3	61	dep	A.	564:565	arg1	describe					576:583	describe	576:583	describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	576:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	6	62	theme	IgG1	1037:1040	arg1	antibody					1042:1049	IgG1 antibody	1037:1049	IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973	1037:1098	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	8	63	theme	further	1513:1519	arg1	design					1529:1534	further vaccine design	1513:1534	further vaccine design	1513:1534	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	0	64	theme	glycosylation	85:97	arg1	system					99:104	an O-linked glycosylation system	73:104	an O-linked glycosylation system in vivo	73:112	Design and production of conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo.					
29423317	0	65	dep	in	106:107	arg1	vivo					109:112	vivo	109:112	vivo	109:112	Design and production of conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo.					
29423317	6	66	theme	conjugate	1007:1015	arg1	vaccine					1017:1023	this conjugate vaccine	1002:1023	this conjugate vaccine	1002:1023	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	6	67	dep	O-antigen	1054:1062	arg1	50973					1094:1098	A strain CMCC 50973	1080:1098	A strain CMCC 50973	1080:1098	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	4	68	theme	A	774:774	arg1	strain					776:781	a recombinant S. Paratyphi A strain	747:781	a recombinant S. Paratyphi A strain	747:781	We successfully generated a recombinant S. Paratyphi A strain with a longer O-polysaccharide chain and transformed the O-linked glycosylation system into the strain.					
29423317	0	69	theme	in	106:107	arg1	system					99:104	an O-linked glycosylation system	73:104	an O-linked glycosylation system in vivo	73:112	Design and production of conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo.					
29423317	2	70	theme	promising	358:366	arg1	approach					368:375	a promising approach	356:375	a promising approach against bacterial infection	356:403	As yet, there are no licensed vaccines against S. Paratyphi A. Biosynthesis of conjugate vaccines has become a promising approach against bacterial infection.					
29423317	5	71	theme	expression	953:962	arg1	vector					964:969	an O-polysaccharide expression vector	933:969	an O-polysaccharide expression vector	933:969	Thus, we avoided the need for construction of an O-polysaccharide expression vector.					
29423317	6	72	theme	strain	1082:1087	arg1	50973					1094:1098	A strain CMCC 50973	1080:1098	A strain CMCC 50973	1080:1098	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	8	73	theme	antibody	1364:1371	arg1	titer					1373:1377	the anti-lipopolysaccharide antibody titer	1336:1377	the anti-lipopolysaccharide antibody titer	1336:1377	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	9	74	theme	economical	1576:1585	arg1	method					1587:1592	an easier and more economical method	1557:1592	an easier and more economical method for the production of S. Paratyphi A conjugate vaccines	1557:1648	Our system provides an easier and more economical method for the production of S. Paratyphi A conjugate vaccines.					
29423317	6	75	theme	Paratyphi	1070:1078	arg1	O-antigen					1054:1062	O-antigen	1054:1062	O-antigen of S. Paratyphi	1054:1078	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	3	76	theme	glycosylation	650:662	arg1	system					664:669	the only currently available glycosylation system	621:669	the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	621:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	3	76	theme	glycosylation	650:662	arg1	approach					611:618	an O-linked glycosylation approach	585:618	an O-linked glycosylation approach	585:618	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	2	77	dep	A.	307:308	arg1	become					349:354	become	349:354	has become a promising approach against bacterial infection	345:403	As yet, there are no licensed vaccines against S. Paratyphi A. Biosynthesis of conjugate vaccines has become a promising approach against bacterial infection.					
29423317	3	78	theme	N-linked	455:462	arg1	systems					478:484	N-linked glycosylation systems	455:484	N-linked glycosylation systems	455:484	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	0	79	theme	vaccines	35:42	arg1	production					11:20	production	11:20	production	11:20	Design and production of conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo.					
29423317	0	79	theme	vaccines	35:42	arg1	Design					0:5	Design	0:5	Design	0:5	Design and production of conjugate vaccines against S. Paratyphi A using an O-linked glycosylation system in vivo.					
29423317	7	80	gly	glycosylation	1255:1267	arg2	Ser					1275:1277	Ser	1275:1277	Ser	1275:1277	Furthermore, we replaced the peptides after the glycosylation site (Ser) with an antigenic peptide (P2).					
29423317	7	80	gly	glycosylation	1255:1267	arg2	site					1269:1272	the glycosylation site	1251:1272	the glycosylation site (Ser)	1251:1278	Furthermore, we replaced the peptides after the glycosylation site (Ser) with an antigenic peptide (P2).					
29423317	7	81	theme	antigenic	1288:1296	arg1	P2					1307:1308	P2	1307:1308	P2	1307:1308	Furthermore, we replaced the peptides after the glycosylation site (Ser) with an antigenic peptide (P2).					
29423317	7	81	theme	antigenic	1288:1296	arg1	peptide					1298:1304	an antigenic peptide	1285:1304	an antigenic peptide (P2)	1285:1309	Furthermore, we replaced the peptides after the glycosylation site (Ser) with an antigenic peptide (P2).					
29423317	8	82	theme	serum	1405:1409	arg1	titer					1373:1377	the anti-lipopolysaccharide antibody titer	1336:1377	the anti-lipopolysaccharide antibody titer	1336:1377	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	8	82	theme	serum	1405:1409	arg1	activity					1393:1400	bactericidal activity	1380:1400	bactericidal activity of serum	1380:1409	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	8	82	theme	serum	1405:1409	arg1	effect					1427:1432	protective effect	1416:1432	protective effect during animal challenge	1416:1456	The results showed that the anti-lipopolysaccharide antibody titer, bactericidal activity of serum, and protective effect during animal challenge could be improved, indicating a potential strategy for further vaccine design.					
29423317	10	83	theme	potential	1708:1716	arg1	approach					1718:1725	a potential approach	1706:1725	a potential approach for the development of next-generation "precise conjugate vaccines	1706:1792	Modification of the glycosylation site sequon provides a potential approach for the development of next-generation "precise conjugate vaccines."					
29423317	9	84	theme	S.	1616:1617	arg1	vaccines					1641:1648	S. Paratyphi A conjugate vaccines	1616:1648	S. Paratyphi A conjugate vaccines	1616:1648	Our system provides an easier and more economical method for the production of S. Paratyphi A conjugate vaccines.					
29423317	3	85	theme	suitable	671:678	arg1	system					664:669	the only currently available glycosylation system	621:669	the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	621:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	3	85	theme	suitable	671:678	arg1	approach					611:618	an O-linked glycosylation approach	585:618	an O-linked glycosylation approach	585:618	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	4	86	theme	longer	790:795	arg1	chain					814:818	a longer O-polysaccharide chain	788:818	a longer O-polysaccharide chain	788:818	We successfully generated a recombinant S. Paratyphi A strain with a longer O-polysaccharide chain and transformed the O-linked glycosylation system into the strain.					
29423317	2	87	theme	vaccines	336:343	arg1	Biosynthesis					310:321	Biosynthesis	310:321	Biosynthesis of conjugate vaccines	310:343	As yet, there are no licensed vaccines against S. Paratyphi A. Biosynthesis of conjugate vaccines has become a promising approach against bacterial infection.					
29423317	9	88	theme	A	1629:1629	arg1	vaccines					1641:1648	S. Paratyphi A conjugate vaccines	1616:1648	S. Paratyphi A conjugate vaccines	1616:1648	Our system provides an easier and more economical method for the production of S. Paratyphi A conjugate vaccines.					
29423317	4	89	link	O-linked	840:847	arg1	system					863:868	the O-linked glycosylation system	836:868	the O-linked glycosylation system	836:868	We successfully generated a recombinant S. Paratyphi A strain with a longer O-polysaccharide chain and transformed the O-linked glycosylation system into the strain.					
29423317	3	90	theme	conjugate	702:710	arg1	vaccine					712:718	an S. Paratyphi A conjugate vaccine	684:718	an S. Paratyphi A conjugate vaccine	684:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	6	91	dep	Paratyphi	1144:1152	arg1	50973					1168:1172	A strain CMCC 50973	1154:1172	S. Paratyphi A strain CMCC 50973	1141:1172	In vivo assays indicated that this conjugate vaccine could evoke IgG1 antibody to O-antigen of S. Paratyphi A strain CMCC 50973 and elicit bactericidal activity against S. Paratyphi A strain CMCC 50973 and five other epidemic strains.					
29423317	3	92	theme	O-polysaccharide	521:536	arg1	structure					538:546	the specialized O-polysaccharide structure	505:546	the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine	505:718	However, the popular biosynthetic strategy using N-linked glycosylation systems does not recognize the specialized O-polysaccharide structure of S. Paratyphi A. Here, we describe an O-linked glycosylation approach, the only currently available glycosylation system suitable for an S. Paratyphi A conjugate vaccine.					
29423317	1	93	theme	infectious	217:226	arg1	worldwide					236:244	a common and serious infectious disease worldwide	196:244	a common and serious infectious disease worldwide	196:244	Enteric fever, mainly caused by Salmonella enterica serovar Paratyphi A, remains a common and serious infectious disease worldwide.					
31029157	3	0	theme	secretome	683:691	arg1	effect					664:669	the effect	660:669	the effect of pVW-MSCs secretome on in vitro angiogenesis	660:716	Moreover, considering the importance of angiogenesis in regenerative mechanisms, we analysed the effect of pVW-MSCs secretome on in vitro angiogenesis.					
31029157	1	1	theme	molecules	275:283	arg1	enriched					253:260	enriched	253:260	enriched	253:260	BACKGROUND MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines.					
31029157	4	2	theme	IL-18	890:894	arg1	IL-10					906:910	IL-6 IL-18 IL-4 IL-2 IL-10	885:910	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.	719:911	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	1	3	theme	growth	286:291	arg1	factors					293:299	growth factors	286:299	growth factors	286:299	BACKGROUND MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines.					
31029157	7	4	theme	secretome	1451:1459	arg1	application					1436:1446	possible future application	1420:1446	possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine	1420:1538	CONCLUSIONS The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.					
31029157	5	5	theme	LPS	1165:1167	arg1	stimulation					1169:1179	the LPS stimulation	1161:1179	the LPS stimulation	1161:1179	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	4	6	theme	immunomodulatory	858:873	arg1	proteins					875:882	other immunomodulatory proteins	852:882	other immunomodulatory proteins	852:882	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	1	7	theme	factors	293:299	arg1	enriched					253:260	enriched	253:260	enriched	253:260	BACKGROUND MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines.					
31029157	7	8	theme	evidence	1380:1387	arg1	lines					1371:1375	The lines	1367:1375	The lines of evidence here presented	1367:1402	CONCLUSIONS The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.					
31029157	6	9	theme	conditioned	1302:1312	arg1	medium					1314:1319	the conditioned medium	1298:1319	the conditioned medium	1298:1319	Conditioned medium from unstimulated pVW-MSCs induced in vitro endothelial angiogenesis, which is more evident when the conditioned medium was from LPS stimulated pVW-MSCs.					
31029157	5	10	theme	GM-CSF	1073:1078	arg1	IL-10					1100:1104	IL-10	1100:1104	IL-10	1100:1104	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	10	theme	GM-CSF	1073:1078	arg1	IFN-γ					1081:1085	IFN-γ	1081:1085	IFN-γ	1081:1085	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	10	theme	GM-CSF	1073:1078	arg1	IL-2					1088:1091	IL-2	1088:1091	IL-2	1088:1091	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	10	theme	GM-CSF	1073:1078	arg1	IL-18					1110:1114	IL-18	1110:1114	IL-18	1110:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	10	theme	GM-CSF	1073:1078	arg1	amount					1063:1068	the amount	1059:1068	the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18	1059:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	10	theme	GM-CSF	1073:1078	arg1	GM-CSF					1073:1078	GM-CSF	1073:1078	GM-CSF	1073:1078	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	10	theme	GM-CSF	1073:1078	arg1	IL-4					1094:1097	IL-4	1094:1097	IL-4	1094:1097	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	4	11	theme	IL-2	901:904	arg1	IL-10					906:910	IL-6 IL-18 IL-4 IL-2 IL-10	885:910	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.	719:911	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	2	12	theme	stimulation	554:564	arg1	presence					538:545	presence	538:545	presence of LPS stimulation	538:564	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	6	13	theme	Conditioned	1182:1192	arg1	medium					1194:1199	Conditioned medium	1182:1199	Conditioned medium from unstimulated pVW-MSCs	1182:1226	Conditioned medium from unstimulated pVW-MSCs induced in vitro endothelial angiogenesis, which is more evident when the conditioned medium was from LPS stimulated pVW-MSCs.					
31029157	3	14	theme	regenerative	623:634	arg1	mechanisms					636:645	regenerative mechanisms	623:645	regenerative mechanisms	623:645	Moreover, considering the importance of angiogenesis in regenerative mechanisms, we analysed the effect of pVW-MSCs secretome on in vitro angiogenesis.					
31029157	5	15	theme	gene	936:939	arg1	expression					941:950	pVW-MSCs gene expression	927:950	pVW-MSCs gene expression	927:950	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	1	16	theme	BACKGROUND	143:152	arg1	secretome					159:167	BACKGROUND MSCs secretome	143:167	BACKGROUND MSCs secretome	143:167	BACKGROUND MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines.					
31029157	4	17	contain	contained	803:811	arg1	medium					769:774	conditioned medium	757:774	conditioned medium from unstimulated pVW-MSCs	757:801	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	4	17	contain	contained	803:811	arg2	levels					818:823	high levels	813:823	high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins	813:882	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	0	18	theme	Stem	79:82	arg1	Cells					84:88	porcine Vascular Wall-Mesenchymal Stem Cells	45:88	porcine Vascular Wall-Mesenchymal Stem Cells	45:88	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	5	19	theme	IL-4	1094:1097	arg1	IL-10					1100:1104	IL-10	1100:1104	IL-10	1100:1104	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	19	theme	IL-4	1094:1097	arg1	IFN-γ					1081:1085	IFN-γ	1081:1085	IFN-γ	1081:1085	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	19	theme	IL-4	1094:1097	arg1	IL-2					1088:1091	IL-2	1088:1091	IL-2	1088:1091	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	19	theme	IL-4	1094:1097	arg1	IL-18					1110:1114	IL-18	1110:1114	IL-18	1110:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	19	theme	IL-4	1094:1097	arg1	amount					1063:1068	the amount	1059:1068	the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18	1059:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	19	theme	IL-4	1094:1097	arg1	GM-CSF					1073:1078	GM-CSF	1073:1078	GM-CSF	1073:1078	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	19	theme	IL-4	1094:1097	arg1	IL-4					1094:1097	IL-4	1094:1097	IL-4	1094:1097	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	3	20	theme	angiogenesis	607:618	arg1	importance					593:602	the importance	589:602	the importance of angiogenesis in regenerative mechanisms	589:645	Moreover, considering the importance of angiogenesis in regenerative mechanisms, we analysed the effect of pVW-MSCs secretome on in vitro angiogenesis.					
31029157	2	21	theme	Stem	498:501	arg1	Cells					503:507	porcine Vascular Wall-Mesenchymal Stem Cells	464:507	porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs)	464:518	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	2	21	theme	Stem	498:501	arg1	pVW-MSCs					510:517	pVW-MSCs	510:517	pVW-MSCs	510:517	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	1	22	theme	cytokines	302:310	arg1	enriched					253:260	enriched	253:260	enriched	253:260	BACKGROUND MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines.					
31029157	5	23	theme	IL-10	1100:1104	arg1	IL-10					1100:1104	IL-10	1100:1104	IL-10	1100:1104	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	23	theme	IL-10	1100:1104	arg1	IFN-γ					1081:1085	IFN-γ	1081:1085	IFN-γ	1081:1085	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	23	theme	IL-10	1100:1104	arg1	IL-2					1088:1091	IL-2	1088:1091	IL-2	1088:1091	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	23	theme	IL-10	1100:1104	arg1	IL-18					1110:1114	IL-18	1110:1114	IL-18	1110:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	23	theme	IL-10	1100:1104	arg1	amount					1063:1068	the amount	1059:1068	the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18	1059:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	23	theme	IL-10	1100:1104	arg1	GM-CSF					1073:1078	GM-CSF	1073:1078	GM-CSF	1073:1078	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	23	theme	IL-10	1100:1104	arg1	IL-4					1094:1097	IL-4	1094:1097	IL-4	1094:1097	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	4	24	from	pVW-MSCs	794:801	arg1	medium					769:774	conditioned medium	757:774	conditioned medium from unstimulated pVW-MSCs	757:801	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	5	25	theme	IFN-γ	1081:1085	arg1	IL-10					1100:1104	IL-10	1100:1104	IL-10	1100:1104	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	25	theme	IFN-γ	1081:1085	arg1	IFN-γ					1081:1085	IFN-γ	1081:1085	IFN-γ	1081:1085	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	25	theme	IFN-γ	1081:1085	arg1	IL-2					1088:1091	IL-2	1088:1091	IL-2	1088:1091	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	25	theme	IFN-γ	1081:1085	arg1	IL-18					1110:1114	IL-18	1110:1114	IL-18	1110:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	25	theme	IFN-γ	1081:1085	arg1	amount					1063:1068	the amount	1059:1068	the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18	1059:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	25	theme	IFN-γ	1081:1085	arg1	GM-CSF					1073:1078	GM-CSF	1073:1078	GM-CSF	1073:1078	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	25	theme	IFN-γ	1081:1085	arg1	IL-4					1094:1097	IL-4	1094:1097	IL-4	1094:1097	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	1	26	theme	MSCs	154:157	arg1	secretome					159:167	BACKGROUND MSCs secretome	143:167	BACKGROUND MSCs secretome	143:167	BACKGROUND MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines.					
31029157	2	27	from	secretome	451:459	arg1	presence					538:545	presence	538:545	presence of LPS stimulation	538:564	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	5	28	theme	IL-2	1088:1091	arg1	IL-10					1100:1104	IL-10	1100:1104	IL-10	1100:1104	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	28	theme	IL-2	1088:1091	arg1	IFN-γ					1081:1085	IFN-γ	1081:1085	IFN-γ	1081:1085	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	28	theme	IL-2	1088:1091	arg1	IL-2					1088:1091	IL-2	1088:1091	IL-2	1088:1091	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	28	theme	IL-2	1088:1091	arg1	IL-18					1110:1114	IL-18	1110:1114	IL-18	1110:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	28	theme	IL-2	1088:1091	arg1	amount					1063:1068	the amount	1059:1068	the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18	1059:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	28	theme	IL-2	1088:1091	arg1	GM-CSF					1073:1078	GM-CSF	1073:1078	GM-CSF	1073:1078	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	28	theme	IL-2	1088:1091	arg1	IL-4					1094:1097	IL-4	1094:1097	IL-4	1094:1097	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	0	29	theme	Constitutive	0:11	arg1	secretome					32:40	Constitutive and LPS-stimulated secretome	0:40	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells	0:88	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	5	30	theme	transient	1137:1145	arg1	decrease					1147:1154	a significant transient decrease	1123:1154	a significant transient decrease with the LPS stimulation	1123:1179	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	4	31	dep	RESULTS	719:725	arg1	IL-10					906:910	IL-6 IL-18 IL-4 IL-2 IL-10	885:910	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.	719:911	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	4	31	dep	RESULTS	719:725	arg1	demonstrated					739:750	demonstrated	739:750	demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins	739:882	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	4	32	theme	conditioned	757:767	arg1	medium					769:774	conditioned medium	757:774	conditioned medium from unstimulated pVW-MSCs	757:801	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	2	33	theme	paper	421:425	arg1	aim					402:404	the leading aim	390:404	the leading aim of the present paper	390:425	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	0	34	theme	LPS-stimulated	17:30	arg1	secretome					32:40	Constitutive and LPS-stimulated secretome	0:40	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells	0:88	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	3	35	dep	in	696:697	arg1	vitro					699:703	vitro	699:703	vitro	699:703	Moreover, considering the importance of angiogenesis in regenerative mechanisms, we analysed the effect of pVW-MSCs secretome on in vitro angiogenesis.					
31029157	2	36	theme	Vascular	472:479	arg1	Cells					503:507	porcine Vascular Wall-Mesenchymal Stem Cells	464:507	porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs)	464:518	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	2	36	theme	Vascular	472:479	arg1	pVW-MSCs					510:517	pVW-MSCs	510:517	pVW-MSCs	510:517	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	3	37	from	importance	593:602	arg1	mechanisms					636:645	regenerative mechanisms	623:645	regenerative mechanisms	623:645	Moreover, considering the importance of angiogenesis in regenerative mechanisms, we analysed the effect of pVW-MSCs secretome on in vitro angiogenesis.					
31029157	1	38	theme	chemokines	316:325	arg1	enriched					253:260	enriched	253:260	enriched	253:260	BACKGROUND MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines.					
31029157	7	39	theme	regenerative	1518:1529	arg1	medicine					1531:1538	translational regenerative medicine	1504:1538	translational regenerative medicine	1504:1538	CONCLUSIONS The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.					
31029157	5	40	theme	IL-8	1028:1031	arg1	increase					1007:1014	a significant increase	993:1014	a significant increase of IL-6 and IL-8	993:1031	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	41	theme	IL-18	1110:1114	arg1	IL-10					1100:1104	IL-10	1100:1104	IL-10	1100:1104	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	41	theme	IL-18	1110:1114	arg1	IFN-γ					1081:1085	IFN-γ	1081:1085	IFN-γ	1081:1085	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	41	theme	IL-18	1110:1114	arg1	IL-2					1088:1091	IL-2	1088:1091	IL-2	1088:1091	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	41	theme	IL-18	1110:1114	arg1	IL-18					1110:1114	IL-18	1110:1114	IL-18	1110:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	41	theme	IL-18	1110:1114	arg1	amount					1063:1068	the amount	1059:1068	the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18	1059:1114	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	41	theme	IL-18	1110:1114	arg1	GM-CSF					1073:1078	GM-CSF	1073:1078	GM-CSF	1073:1078	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	41	theme	IL-18	1110:1114	arg1	IL-4					1094:1097	IL-4	1094:1097	IL-4	1094:1097	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	0	42	theme	Vascular	53:60	arg1	Cells					84:88	porcine Vascular Wall-Mesenchymal Stem Cells	45:88	porcine Vascular Wall-Mesenchymal Stem Cells	45:88	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	3	43	theme	pVW-MSCs	674:681	arg1	secretome					683:691	pVW-MSCs secretome	674:691	pVW-MSCs secretome	674:691	Moreover, considering the importance of angiogenesis in regenerative mechanisms, we analysed the effect of pVW-MSCs secretome on in vitro angiogenesis.					
31029157	4	44	theme	high	813:816	arg1	levels					818:823	high levels	813:823	high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins	813:882	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	7	45	theme	research	1484:1491	arg1	studies					1493:1499	research studies	1484:1499	research studies in translational regenerative medicine	1484:1538	CONCLUSIONS The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.					
31029157	2	46	from	change	528:533	arg1	presence					538:545	presence	538:545	presence of LPS stimulation	538:564	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	2	47	theme	model	383:387	arg1	value					366:370	the translational value	348:370	the translational value of the pig model	348:387	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	5	48	located	observed	1037:1044	arg1	particular					982:991	particular	982:991	particular	982:991	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	5	48	located	observed	1037:1044	arg2	increase					1007:1014	a significant increase	993:1014	a significant increase of IL-6 and IL-8	993:1031	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	6	49	dep	induced	1228:1234	arg1	evident					1285:1291	evident	1285:1291	evident	1285:1291	Conditioned medium from unstimulated pVW-MSCs induced in vitro endothelial angiogenesis, which is more evident when the conditioned medium was from LPS stimulated pVW-MSCs.					
31029157	5	50	theme	IL-6	1019:1022	arg1	increase					1007:1014	a significant increase	993:1014	a significant increase of IL-6 and IL-8	993:1031	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	1	51	theme	bioactive	265:273	arg1	molecules					275:283	bioactive molecules	265:283	bioactive molecules	265:283	BACKGROUND MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines.					
31029157	4	52	theme	proteins	875:882	arg1	levels					818:823	high levels	813:823	high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins	813:882	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	4	53	theme	IL-6	885:888	arg1	IL-10					906:910	IL-6 IL-18 IL-4 IL-2 IL-10	885:910	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.	719:911	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	7	54	theme	possible	1420:1427	arg1	application					1436:1446	possible future application	1420:1446	possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine	1420:1538	CONCLUSIONS The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.					
31029157	6	55	theme	endothelial	1245:1255	arg1	angiogenesis					1257:1268	in vitro endothelial angiogenesis	1236:1268	in vitro endothelial angiogenesis	1236:1268	Conditioned medium from unstimulated pVW-MSCs induced in vitro endothelial angiogenesis, which is more evident when the conditioned medium was from LPS stimulated pVW-MSCs.					
31029157	7	56	dep	CONCLUSIONS	1355:1365	arg1	shed					1404:1407	shed	1404:1407	shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine	1404:1538	CONCLUSIONS The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.					
31029157	7	57	theme	future	1429:1434	arg1	application					1436:1446	possible future application	1420:1446	possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine	1420:1538	CONCLUSIONS The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.					
31029157	4	58	theme	IFN-γ	842:846	arg1	levels					818:823	high levels	813:823	high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins	813:882	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	6	59	theme	in	1236:1237	arg1	angiogenesis					1257:1268	in vitro endothelial angiogenesis	1236:1268	in vitro endothelial angiogenesis	1236:1268	Conditioned medium from unstimulated pVW-MSCs induced in vitro endothelial angiogenesis, which is more evident when the conditioned medium was from LPS stimulated pVW-MSCs.					
31029157	4	60	theme	other	852:856	arg1	proteins					875:882	other immunomodulatory proteins	852:882	other immunomodulatory proteins	852:882	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	5	61	with	decrease	1147:1154	arg1	stimulation					1169:1179	the LPS stimulation	1161:1179	the LPS stimulation	1161:1179	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	1	62	theme	whole-cell-based	213:228	arg1	therapies					230:238	whole-cell-based therapies	213:238	whole-cell-based therapies	213:238	BACKGROUND MSCs secretome is under investigation as an alternative to whole-cell-based therapies, since it is enriched of bioactive molecules: growth factors, cytokines and chemokines.					
31029157	6	63	theme	unstimulated	1206:1217	arg1	pVW-MSCs					1219:1226	unstimulated pVW-MSCs	1206:1226	unstimulated pVW-MSCs	1206:1226	Conditioned medium from unstimulated pVW-MSCs induced in vitro endothelial angiogenesis, which is more evident when the conditioned medium was from LPS stimulated pVW-MSCs.					
31029157	4	64	theme	IL-4	896:899	arg1	IL-10					906:910	IL-6 IL-18 IL-4 IL-2 IL-10	885:910	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.	719:911	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	5	65	theme	pVW-MSCs	927:934	arg1	expression					941:950	pVW-MSCs gene expression	927:950	pVW-MSCs gene expression	927:950	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	2	66	theme	LPS	550:552	arg1	stimulation					554:564	LPS stimulation	550:564	LPS stimulation	550:564	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	6	67	from	pVW-MSCs	1219:1226	arg1	medium					1194:1199	Conditioned medium	1182:1199	Conditioned medium from unstimulated pVW-MSCs	1182:1226	Conditioned medium from unstimulated pVW-MSCs induced in vitro endothelial angiogenesis, which is more evident when the conditioned medium was from LPS stimulated pVW-MSCs.					
31029157	0	68	theme	Cells	84:88	arg1	secretome					32:40	Constitutive and LPS-stimulated secretome	0:40	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells	0:88	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	2	69	theme	Cells	503:507	arg1	change					528:533	its change	524:533	its change in presence of LPS stimulation	524:564	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	2	69	theme	Cells	503:507	arg1	secretome					451:459	the secretome	447:459	the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs)	447:518	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	2	70	theme	Wall-Mesenchymal	481:496	arg1	Cells					503:507	porcine Vascular Wall-Mesenchymal Stem Cells	464:507	porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs)	464:518	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	2	70	theme	Wall-Mesenchymal	481:496	arg1	pVW-MSCs					510:517	pVW-MSCs	510:517	pVW-MSCs	510:517	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	0	71	theme	in	108:109	arg1	angiogenesis					129:140	in vitro endothelial angiogenesis	108:140	in vitro endothelial angiogenesis	108:140	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	0	72	theme	endothelial	117:127	arg1	angiogenesis					129:140	in vitro endothelial angiogenesis	108:140	in vitro endothelial angiogenesis	108:140	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	0	73	dep	in	108:109	arg1	vitro					111:115	vitro	111:115	vitro	111:115	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	4	74	theme	unstimulated	781:792	arg1	pVW-MSCs					794:801	unstimulated pVW-MSCs	781:801	unstimulated pVW-MSCs	781:801	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	2	75	theme	leading	394:400	arg1	aim					402:404	the leading aim	390:404	the leading aim of the present paper	390:425	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	5	76	theme	secretome	956:964	arg1	composition					966:976	secretome composition	956:976	secretome composition	956:976	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	2	77	theme	present	413:419	arg1	paper					421:425	the present paper	409:425	the present paper	409:425	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	0	78	theme	porcine	45:51	arg1	Cells					84:88	porcine Vascular Wall-Mesenchymal Stem Cells	45:88	porcine Vascular Wall-Mesenchymal Stem Cells	45:88	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	2	79	theme	porcine	464:470	arg1	Cells					503:507	porcine Vascular Wall-Mesenchymal Stem Cells	464:507	porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs)	464:518	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	2	79	theme	porcine	464:470	arg1	pVW-MSCs					510:517	pVW-MSCs	510:517	pVW-MSCs	510:517	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	4	80	theme	IL-8	828:831	arg1	levels					818:823	high levels	813:823	high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins	813:882	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	0	81	theme	Wall-Mesenchymal	62:77	arg1	Cells					84:88	porcine Vascular Wall-Mesenchymal Stem Cells	45:88	porcine Vascular Wall-Mesenchymal Stem Cells	45:88	Constitutive and LPS-stimulated secretome of porcine Vascular Wall-Mesenchymal Stem Cells exerts effects on in vitro endothelial angiogenesis.					
31029157	7	82	from	studies	1493:1499	arg1	medicine					1531:1538	translational regenerative medicine	1504:1538	translational regenerative medicine	1504:1538	CONCLUSIONS The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.					
31029157	4	83	theme	GM-CSF	834:839	arg1	levels					818:823	high levels	813:823	high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins	813:882	RESULTS Our results demonstrated that conditioned medium from unstimulated pVW-MSCs contained high levels of IL-8, GM-CSF, IFN-γ and other immunomodulatory proteins: IL-6 IL-18 IL-4 IL-2 IL-10.					
31029157	2	84	theme	translational	352:364	arg1	value					366:370	the translational value	348:370	the translational value of the pig model	348:387	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31029157	5	85	theme	significant	1125:1135	arg1	decrease					1147:1154	a significant transient decrease	1123:1154	a significant transient decrease with the LPS stimulation	1123:1179	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	7	86	theme	translational	1504:1516	arg1	medicine					1531:1538	translational regenerative medicine	1504:1538	translational regenerative medicine	1504:1538	CONCLUSIONS The lines of evidence here presented shed a light on possible future application of secretome derived by pVW-MSCs on research studies in translational regenerative medicine.					
31029157	5	87	theme	significant	995:1005	arg1	increase					1007:1014	a significant increase	993:1014	a significant increase of IL-6 and IL-8	993:1031	LPS modulates pVW-MSCs gene expression and secretome composition, in particular a significant increase of IL-6 and IL-8 was observed; conversely, the amount of GM-CSF, IFN-γ, IL-2, IL-4, IL-10 and IL-18 showed a significant transient decrease with the LPS stimulation.					
31029157	6	88	dep	in	1236:1237	arg1	vitro					1239:1243	vitro	1239:1243	vitro	1239:1243	Conditioned medium from unstimulated pVW-MSCs induced in vitro endothelial angiogenesis, which is more evident when the conditioned medium was from LPS stimulated pVW-MSCs.					
31029157	3	89	from	effect	664:669	arg1	angiogenesis					705:716	in vitro angiogenesis	696:716	in vitro angiogenesis	696:716	Moreover, considering the importance of angiogenesis in regenerative mechanisms, we analysed the effect of pVW-MSCs secretome on in vitro angiogenesis.					
31029157	3	90	theme	in	696:697	arg1	angiogenesis					705:716	in vitro angiogenesis	696:716	in vitro angiogenesis	696:716	Moreover, considering the importance of angiogenesis in regenerative mechanisms, we analysed the effect of pVW-MSCs secretome on in vitro angiogenesis.					
31029157	2	91	theme	pig	379:381	arg1	model					383:387	the pig model	375:387	the pig model	375:387	Taking into account the translational value of the pig model, the leading aim of the present paper was to characterize the secretome of porcine Vascular Wall-Mesenchymal Stem Cells (pVW-MSCs) and its change in presence of LPS stimulation.					
31234557	2	0	from	KF-1	356:359	arg1	pppel9a					319:325	pppel9a	319:325	pppel9a	319:325	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	2	0	from	KF-1	356:359	arg1	cloning					276:282	the cloning	272:282	the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1	272:359	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	2	0	from	KF-1	356:359	arg1	gene					313:316	an alkaline pectate lyase gene	287:316	an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1	287:359	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	7	1	attach	isolated	1022:1029	arg2	pectins					1014:1020	water-soluble pectins	1000:1020	water-soluble pectins isolated from different plants	1000:1051	In addition, PpPel9a was active against water-soluble pectins isolated from different plants.					
31234557	7	1	attach	isolated	1022:1029	arg1	plants					1046:1051	different plants	1036:1051	different plants	1036:1051	In addition, PpPel9a was active against water-soluble pectins isolated from different plants.					
31234557	10	2	theme	spectrometer	1447:1458	arg1	analyses					1501:1508	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses	1393:1508	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses	1393:1508	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	2	3	theme	alkaline	290:297	arg1	pppel9a					319:325	pppel9a	319:325	pppel9a	319:325	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	2	3	theme	alkaline	290:297	arg1	gene					313:316	an alkaline pectate lyase gene	287:316	an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1	287:359	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	9	4	theme	pectin	1332:1337	arg1	mass					1317:1320	the molecular mass	1303:1320	the molecular mass of citrus pectin (~230.2 kDa)	1303:1350	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	7	5	from	addition	963:970	arg1	active					985:990	active	985:990	active	985:990	In addition, PpPel9a was active against water-soluble pectins isolated from different plants.					
31234557	10	6	theme	composition	1489:1499	arg1	analyses					1501:1508	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses	1393:1508	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses	1393:1508	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	4	7	theme	single	582:587	arg1	step					589:592	a single step	580:592	a single step using Ni2+-NTA affinity chromatography	580:631	Recombinant PpPel9a produced in Escherichia coli was purified to electrophoretic homogeneity in a single step using Ni2+-NTA affinity chromatography.					
31234557	11	8	dep	In	1626:1627	arg1	vitro					1629:1633	vitro	1629:1633	vitro	1629:1633	In vitro testing showed that the degradation product MCP-0.3 significantly promotes the growth of Lactobacillus plantarum and L. rhamnosus.					
31234557	3	9	theme	lyase	462:466	arg1	family					468:473	the polysaccharide lyase family 9	443:475	the polysaccharide lyase family 9 (PL9)	443:481	The full-length gene (1350 bp) encodes for a 449-residue protein that belongs to the polysaccharide lyase family 9 (PL9).					
31234557	3	9	theme	lyase	462:466	arg1	PL9					478:480	PL9	478:480	PL9	478:480	The full-length gene (1350 bp) encodes for a 449-residue protein that belongs to the polysaccharide lyase family 9 (PL9).					
31234557	1	10	theme	important	147:155	arg1	role					157:160	an important role	144:160	an important role	144:160	Pectate lyases play an important role in pectin degradation, and therefore are highly useful in the food and textile industries.					
31234557	8	11	theme	citrus	1084:1089	arg1	pectin					1091:1096	citrus pectin	1084:1096	citrus pectin	1084:1096	Using this lyase, we degraded citrus pectin, purified fractions using Diethylaminoethyl (DEAE)-sepharose column chromatography, and characterized the main fraction MCP-0.3.					
31234557	1	12	theme	textile	233:239	arg1	industries					241:250	the food and textile industries	220:250	the food and textile industries	220:250	Pectate lyases play an important role in pectin degradation, and therefore are highly useful in the food and textile industries.					
31234557	8	13	theme	-sepharose	1148:1157	arg1	chromatography					1166:1179	Diethylaminoethyl (DEAE)-sepharose column chromatography	1124:1179	Diethylaminoethyl (DEAE)-sepharose column chromatography	1124:1179	Using this lyase, we degraded citrus pectin, purified fractions using Diethylaminoethyl (DEAE)-sepharose column chromatography, and characterized the main fraction MCP-0.3.					
31234557	1	14	from	industries	241:250	arg1	useful					210:215	useful	210:215	useful	210:215	Pectate lyases play an important role in pectin degradation, and therefore are highly useful in the food and textile industries.					
31234557	4	15	theme	electrophoretic	549:563	arg1	homogeneity					565:575	electrophoretic homogeneity	549:575	electrophoretic homogeneity	549:575	Recombinant PpPel9a produced in Escherichia coli was purified to electrophoretic homogeneity in a single step using Ni2+-NTA affinity chromatography.					
31234557	5	16	theme	enzyme	638:643	arg1	optimal					721:727	optimal	721:727	optimal	721:727	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	16	theme	enzyme	638:643	arg1	activity					645:652	The enzyme activity	634:652	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa)	634:703	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	0	17	from	Cloning	60:66	arg1	Degradation					111:121	Pectin Degradation	104:121	Pectin Degradation	104:121	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	10	18	theme	endo-pectate	1549:1560	arg1	lyase					1562:1566	an endo-pectate lyase	1546:1566	an endo-pectate lyase	1546:1566	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	11	19	theme	rhamnosus	1755:1763	arg1	growth					1714:1719	the growth	1710:1719	the growth of Lactobacillus plantarum and L. rhamnosus	1710:1763	In vitro testing showed that the degradation product MCP-0.3 significantly promotes the growth of Lactobacillus plantarum and L. rhamnosus.					
31234557	0	20	theme	Pectin	104:109	arg1	Degradation					111:121	Pectin Degradation	104:121	Pectin Degradation	104:121	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	8	21	theme	Diethylaminoethyl	1124:1140	arg1	-sepharose					1148:1157	Diethylaminoethyl (DEAE)-sepharose	1124:1157	Diethylaminoethyl (DEAE)-sepharose column chromatography	1124:1179	Using this lyase, we degraded citrus pectin, purified fractions using Diethylaminoethyl (DEAE)-sepharose column chromatography, and characterized the main fraction MCP-0.3.					
31234557	0	22	from	KF-1	54:57	arg1	Lyase					20:24	A Novel PL9 Pectate Lyase	0:24	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.	0:122	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	1	23	from	useful	210:215	arg1	industries					241:250	the food and textile industries	220:250	the food and textile industries	220:250	Pectate lyases play an important role in pectin degradation, and therefore are highly useful in the food and textile industries.					
31234557	5	24	dep	PpPel9a	657:663	arg1	weight					685:690	apparent molecular weight	666:690	apparent molecular weight of 45.3 kDa	666:702	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	8	25	theme	fraction	1209:1216	arg1	MCP-0.3					1218:1224	the main fraction MCP-0.3	1200:1224	the main fraction MCP-0.3	1200:1224	Using this lyase, we degraded citrus pectin, purified fractions using Diethylaminoethyl (DEAE)-sepharose column chromatography, and characterized the main fraction MCP-0.3.					
31234557	11	26	theme	degradation	1659:1669	arg1	product					1671:1677	the degradation product MCP-0.3	1655:1685	the degradation product MCP-0.3	1655:1685	In vitro testing showed that the degradation product MCP-0.3 significantly promotes the growth of Lactobacillus plantarum and L. rhamnosus.					
31234557	5	27	theme	vis-à-vis	816:824	arg1	pectins					859:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	0	28	from	Expression	69:78	arg1	Degradation					111:121	Pectin Degradation	104:121	Pectin Degradation	104:121	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	4	29	theme	affinity	609:616	arg1	chromatography					618:631	Ni2+-NTA affinity chromatography	600:631	Ni2+-NTA affinity chromatography	600:631	Recombinant PpPel9a produced in Escherichia coli was purified to electrophoretic homogeneity in a single step using Ni2+-NTA affinity chromatography.					
31234557	5	30	theme	homogalacturonan	781:796	arg1	type					798:801	homogalacturonan type	781:801	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	30	theme	homogalacturonan	781:796	arg1	HG					804:805	HG	804:805	HG	804:805	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	31	theme	kDa	700:702	arg1	weight					685:690	apparent molecular weight	666:690	apparent molecular weight of 45.3 kDa	666:702	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	12	32	theme	pectin	1854:1859	arg1	products					1861:1868	pharmacologically active pectin products	1829:1868	pharmacologically active pectin products	1829:1868	In this regard, the enzyme has potential in the preparation of pharmacologically active pectin products.					
31234557	6	33	theme	HG-type	874:880	arg1	pectins					882:888	HG-type pectins	874:888	HG-type pectins	874:888	Using HG-type pectins as substrate, PpPel9a showed greater activity with de-esterified HGs.					
31234557	12	34	contain	has	1793:1795	arg2	potential					1797:1805	potential	1797:1805	potential	1797:1805	In this regard, the enzyme has potential in the preparation of pharmacologically active pectin products.					
31234557	12	34	contain	has	1793:1795	arg1	enzyme					1786:1791	the enzyme	1782:1791	the enzyme	1782:1791	In this regard, the enzyme has potential in the preparation of pharmacologically active pectin products.					
31234557	5	35	theme	molecular	675:683	arg1	weight					685:690	apparent molecular weight	666:690	apparent molecular weight of 45.3 kDa	666:702	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	9	36	theme	High-performance	1227:1242	arg1	HPGPC					1275:1279	HPGPC	1275:1279	HPGPC	1275:1279	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	9	36	theme	High-performance	1227:1242	arg1	chromatography					1259:1272	High-performance gel permeation chromatography	1227:1272	High-performance gel permeation chromatography (HPGPC) analysis	1227:1289	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	9	37	theme	permeation	1248:1257	arg1	HPGPC					1275:1279	HPGPC	1275:1279	HPGPC	1275:1279	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	9	37	theme	permeation	1248:1257	arg1	chromatography					1259:1272	High-performance gel permeation chromatography	1227:1272	High-performance gel permeation chromatography (HPGPC) analysis	1227:1289	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	10	38	theme	liquid	1411:1416	arg1	UPLC-MS					1461:1467	UPLC-MS	1461:1467	UPLC-MS	1461:1467	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	10	38	theme	liquid	1411:1416	arg1	chromatography					1418:1431	Ultra-performance liquid chromatography	1393:1431	Ultra-performance liquid chromatography	1393:1431	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	0	39	theme	PL9	8:10	arg1	Lyase					20:24	A Novel PL9 Pectate Lyase	0:24	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.	0:122	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	6	40	theme	de-esterified	941:953	arg1	HGs					955:957	de-esterified HGs	941:957	de-esterified HGs	941:957	Using HG-type pectins as substrate, PpPel9a showed greater activity with de-esterified HGs.					
31234557	2	41	theme	polymyxa	347:354	arg1	KF-1					356:359	Paenibacillus polymyxa KF-1	333:359	Paenibacillus polymyxa KF-1	333:359	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	8	42	theme	DEAE	1143:1146	arg1	-sepharose					1148:1157	Diethylaminoethyl (DEAE)-sepharose	1124:1157	Diethylaminoethyl (DEAE)-sepharose column chromatography	1124:1179	Using this lyase, we degraded citrus pectin, purified fractions using Diethylaminoethyl (DEAE)-sepharose column chromatography, and characterized the main fraction MCP-0.3.					
31234557	10	43	theme	mass	1442:1445	arg1	spectrometer					1447:1458	tandem mass spectrometer	1435:1458	tandem mass spectrometer	1435:1458	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	3	44	theme	449-residue	407:417	arg1	protein					419:425	a 449-residue protein	405:425	a 449-residue protein that belongs to the polysaccharide lyase family 9 (PL9)	405:481	The full-length gene (1350 bp) encodes for a 449-residue protein that belongs to the polysaccharide lyase family 9 (PL9).					
31234557	0	45	theme	Paenibacillus	31:43	arg1	KF-1					54:57	Paenibacillus polymyxa KF-1	31:57	Paenibacillus polymyxa KF-1	31:57	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	0	46	dep	Lyase	20:24	arg1	Cloning					60:66	Cloning	60:66	Cloning	60:66	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	0	46	dep	Lyase	20:24	arg1	Application					89:99	Its Application	85:99	Its Application in Pectin Degradation	85:121	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	0	46	dep	Lyase	20:24	arg1	Expression					69:78	Expression	69:78	Expression	69:78	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	10	47	theme	citrus	1611:1616	arg1	pectin					1618:1623	citrus pectin	1611:1623	citrus pectin	1611:1623	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	1	48	theme	Pectate	124:130	arg1	lyases					132:137	Pectate lyases	124:137	Pectate lyases	124:137	Pectate lyases play an important role in pectin degradation, and therefore are highly useful in the food and textile industries.					
31234557	2	49	theme	gene	313:316	arg1	cloning					276:282	the cloning	272:282	the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1	272:359	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	7	50	theme	different	1036:1044	arg1	plants					1046:1051	different plants	1036:1051	different plants	1036:1051	In addition, PpPel9a was active against water-soluble pectins isolated from different plants.					
31234557	0	51	from	Application	89:99	arg1	Degradation					111:121	Pectin Degradation	104:121	Pectin Degradation	104:121	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	2	52	theme	pectate	299:305	arg1	pppel9a					319:325	pppel9a	319:325	pppel9a	319:325	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	2	52	theme	pectate	299:305	arg1	gene					313:316	an alkaline pectate lyase gene	287:316	an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1	287:359	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	9	53	theme	citrus	1325:1330	arg1	pectin					1332:1337	citrus pectin	1325:1337	citrus pectin (~230.2 kDa)	1325:1350	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	9	53	theme	citrus	1325:1330	arg1	kDa					1347:1349	~230.2 kDa	1340:1349	~230.2 kDa	1340:1349	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	2	54	from	cloning	276:282	arg1	KF-1					356:359	Paenibacillus polymyxa KF-1	333:359	Paenibacillus polymyxa KF-1	333:359	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	4	55	theme	Recombinant	484:494	arg1	PpPel9a					496:502	Recombinant PpPel9a	484:502	Recombinant PpPel9a produced in Escherichia coli	484:531	Recombinant PpPel9a produced in Escherichia coli was purified to electrophoretic homogeneity in a single step using Ni2+-NTA affinity chromatography.					
31234557	7	56	theme	water-soluble	1000:1012	arg1	pectins					1014:1020	water-soluble pectins	1000:1020	water-soluble pectins isolated from different plants	1000:1051	In addition, PpPel9a was active against water-soluble pectins isolated from different plants.					
31234557	1	57	theme	food	224:227	arg1	industries					241:250	the food and textile industries	220:250	the food and textile industries	220:250	Pectate lyases play an important role in pectin degradation, and therefore are highly useful in the food and textile industries.					
31234557	10	58	theme	monosaccharide	1474:1487	arg1	composition					1489:1499	monosaccharide composition	1474:1499	monosaccharide composition	1474:1499	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	3	59	theme	polysaccharide	447:460	arg1	family					468:473	the polysaccharide lyase family 9	443:475	the polysaccharide lyase family 9 (PL9)	443:481	The full-length gene (1350 bp) encodes for a 449-residue protein that belongs to the polysaccharide lyase family 9 (PL9).					
31234557	3	59	theme	polysaccharide	447:460	arg1	PL9					478:480	PL9	478:480	PL9	478:480	The full-length gene (1350 bp) encodes for a 449-residue protein that belongs to the polysaccharide lyase family 9 (PL9).					
31234557	5	60	theme	substrate	756:764	arg1	preference					766:775	substrate preference	756:775	substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	756:865	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	11	61	theme	plantarum	1738:1746	arg1	growth					1714:1719	the growth	1710:1719	the growth of Lactobacillus plantarum and L. rhamnosus	1710:1763	In vitro testing showed that the degradation product MCP-0.3 significantly promotes the growth of Lactobacillus plantarum and L. rhamnosus.					
31234557	1	62	theme	pectin	165:170	arg1	degradation					172:182	pectin degradation	165:182	pectin degradation	165:182	Pectate lyases play an important role in pectin degradation, and therefore are highly useful in the food and textile industries.					
31234557	5	63	with	optimal	721:727	arg1	preference					766:775	substrate preference	756:775	substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	756:865	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	8	64	theme	column	1159:1164	arg1	chromatography					1166:1179	Diethylaminoethyl (DEAE)-sepharose column chromatography	1124:1179	Diethylaminoethyl (DEAE)-sepharose column chromatography	1124:1179	Using this lyase, we degraded citrus pectin, purified fractions using Diethylaminoethyl (DEAE)-sepharose column chromatography, and characterized the main fraction MCP-0.3.					
31234557	5	65	from	°C	747:748	arg1	optimal					721:727	optimal	721:727	optimal	721:727	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	65	from	°C	747:748	arg1	activity					645:652	The enzyme activity	634:652	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa)	634:703	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	66	theme	pectins	808:814	arg1	pectins					859:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	6	67	theme	greater	919:925	arg1	activity					927:934	greater activity	919:934	greater activity	919:934	Using HG-type pectins as substrate, PpPel9a showed greater activity with de-esterified HGs.					
31234557	8	68	theme	main	1204:1207	arg1	MCP-0.3					1218:1224	the main fraction MCP-0.3	1200:1224	the main fraction MCP-0.3	1200:1224	Using this lyase, we degraded citrus pectin, purified fractions using Diethylaminoethyl (DEAE)-sepharose column chromatography, and characterized the main fraction MCP-0.3.					
31234557	4	69	theme	Ni2+-NTA	600:607	arg1	chromatography					618:631	Ni2+-NTA affinity chromatography	600:631	Ni2+-NTA affinity chromatography	600:631	Recombinant PpPel9a produced in Escherichia coli was purified to electrophoretic homogeneity in a single step using Ni2+-NTA affinity chromatography.					
31234557	12	70	theme	active	1847:1852	arg1	products					1861:1868	pharmacologically active pectin products	1829:1868	pharmacologically active pectin products	1829:1868	In this regard, the enzyme has potential in the preparation of pharmacologically active pectin products.					
31234557	5	71	theme	type	798:801	arg1	pectins					859:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	72	theme	PpPel9a	657:663	arg1	optimal					721:727	optimal	721:727	optimal	721:727	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	72	theme	PpPel9a	657:663	arg1	activity					645:652	The enzyme activity	634:652	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa)	634:703	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	12	73	theme	products	1861:1868	arg1	preparation					1814:1824	the preparation	1810:1824	the preparation of pharmacologically active pectin products	1810:1868	In this regard, the enzyme has potential in the preparation of pharmacologically active pectin products.					
31234557	5	74	theme	type	854:857	arg1	pectins					859:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	75	theme	apparent	666:673	arg1	weight					685:690	apparent molecular weight	666:690	apparent molecular weight of 45.3 kDa	666:702	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	76	from	pH	732:733	arg1	optimal					721:727	optimal	721:727	optimal	721:727	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	5	76	from	pH	732:733	arg1	activity					645:652	The enzyme activity	634:652	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa)	634:703	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	0	77	theme	Novel	2:6	arg1	Lyase					20:24	A Novel PL9 Pectate Lyase	0:24	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.	0:122	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	9	78	theme	gel	1244:1246	arg1	HPGPC					1275:1279	HPGPC	1275:1279	HPGPC	1275:1279	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	9	78	theme	gel	1244:1246	arg1	chromatography					1259:1272	High-performance gel permeation chromatography	1227:1272	High-performance gel permeation chromatography (HPGPC) analysis	1227:1289	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	5	79	theme	rhamnogalacturonan-I	826:845	arg1	pectins					859:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	3	80	theme	full-length	366:376	arg1	gene					378:381	The full-length gene	362:381	The full-length gene (1350 bp)	362:391	The full-length gene (1350 bp) encodes for a 449-residue protein that belongs to the polysaccharide lyase family 9 (PL9).					
31234557	3	80	theme	full-length	366:376	arg1	bp					389:390	1350 bp	384:390	1350 bp	384:390	The full-length gene (1350 bp) encodes for a 449-residue protein that belongs to the polysaccharide lyase family 9 (PL9).					
31234557	0	81	theme	Pectate	12:18	arg1	Lyase					20:24	A Novel PL9 Pectate Lyase	0:24	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.	0:122	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	9	82	theme	chromatography	1259:1272	arg1	analysis					1282:1289	High-performance gel permeation chromatography (HPGPC) analysis	1227:1289	High-performance gel permeation chromatography (HPGPC) analysis	1227:1289	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	5	83	theme	RG-I	848:851	arg1	pectins					859:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins	781:865	The enzyme activity of PpPel9a (apparent molecular weight of 45.3 kDa) was found to be optimal at pH 10.0 and 40 °C, with substrate preference for homogalacturonan type (HG) pectins vis-à-vis rhamnogalacturonan-I (RG-I) type pectins.					
31234557	7	84	from	active	985:990	arg1	addition					963:970	addition	963:970	addition	963:970	In addition, PpPel9a was active against water-soluble pectins isolated from different plants.					
31234557	10	85	theme	Ultra-performance	1393:1409	arg1	UPLC-MS					1461:1467	UPLC-MS	1461:1467	UPLC-MS	1461:1467	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	10	85	theme	Ultra-performance	1393:1409	arg1	chromatography					1418:1431	Ultra-performance liquid chromatography	1393:1431	Ultra-performance liquid chromatography	1393:1431	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	10	86	theme	HG	1598:1599	arg1	pectin					1618:1623	citrus pectin	1611:1623	citrus pectin	1611:1623	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	10	86	theme	HG	1598:1599	arg1	domain					1601:1606	the HG domain	1594:1606	the HG domain of citrus pectin	1594:1623	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	2	87	theme	Paenibacillus	333:345	arg1	KF-1					356:359	Paenibacillus polymyxa KF-1	333:359	Paenibacillus polymyxa KF-1	333:359	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	0	88	theme	polymyxa	45:52	arg1	KF-1					54:57	Paenibacillus polymyxa KF-1	31:57	Paenibacillus polymyxa KF-1	31:57	A Novel PL9 Pectate Lyase from Paenibacillus polymyxa KF-1: Cloning, Expression, and Its Application in Pectin Degradation.					
31234557	10	89	theme	tandem	1435:1440	arg1	spectrometer					1447:1458	tandem mass spectrometer	1435:1458	tandem mass spectrometer	1435:1458	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	10	90	theme	pectin	1618:1623	arg1	pectin					1618:1623	citrus pectin	1611:1623	citrus pectin	1611:1623	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	10	90	theme	pectin	1618:1623	arg1	domain					1601:1606	the HG domain	1594:1606	the HG domain of citrus pectin	1594:1623	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	10	91	theme	chromatography	1418:1431	arg1	analyses					1501:1508	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses	1393:1508	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses	1393:1508	Ultra-performance liquid chromatography - tandem mass spectrometer (UPLC-MS) and monosaccharide composition analyses demonstrated that PpPel9a worked as an endo-pectate lyase, which acted primarily on the HG domain of citrus pectin.					
31234557	11	92	theme	In	1626:1627	arg1	testing					1635:1641	In vitro testing	1626:1641	In vitro testing	1626:1641	In vitro testing showed that the degradation product MCP-0.3 significantly promotes the growth of Lactobacillus plantarum and L. rhamnosus.					
31234557	9	93	theme	molecular	1307:1315	arg1	mass					1317:1320	the molecular mass	1303:1320	the molecular mass of citrus pectin (~230.2 kDa)	1303:1350	High-performance gel permeation chromatography (HPGPC) analysis showed that the molecular mass of citrus pectin (~230.2 kDa) was reduced to ~24 kDa upon degradation.					
31234557	2	94	theme	lyase	307:311	arg1	pppel9a					319:325	pppel9a	319:325	pppel9a	319:325	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31234557	2	94	theme	lyase	307:311	arg1	gene					313:316	an alkaline pectate lyase gene	287:316	an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1	287:359	Here, we report on the cloning of an alkaline pectate lyase gene (pppel9a) from Paenibacillus polymyxa KF-1.					
31524627	1	0	theme	blue	305:308	arg1	methylene					295:303	methylene blue	295:308	methylene blue (MB)	295:313	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	1	0	theme	blue	305:308	arg1	MB					311:312	MB	311:312	MB	311:312	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	1	1	theme	methyl	238:243	arg1	orange					245:250	methyl orange	238:250	methyl orange (MO)	238:255	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	1	1	theme	methyl	238:243	arg1	MO					253:254	MO	253:254	MO	253:254	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	0	2	from	removal	9:15	arg1	gel					73:75	exfoliated montmorillonite/chitosan gel	37:75	exfoliated montmorillonite/chitosan gel	37:75	Enhanced removal of methyl orange on exfoliated montmorillonite/chitosan gel in presence of methylene blue.					
31524627	6	3	theme	enhanced	1173:1180	arg1	purification					1182:1193	the enhanced purification	1169:1193	the enhanced purification of the wastewater with multiple dyes	1169:1230	This finding of the synergistic effect between the two dyes on the proposed composite opens up new vistas to imagine the enhanced purification of the wastewater with multiple dyes co-existed using the multifunctional adsorbents.					
31524627	0	4	theme	blue	102:105	arg1	methylene					92:100	methylene blue	92:105	methylene blue	92:105	Enhanced removal of methyl orange on exfoliated montmorillonite/chitosan gel in presence of methylene blue.					
31524627	0	5	attach	removal	9:15	arg3	gel					73:75	exfoliated montmorillonite/chitosan gel	37:75	exfoliated montmorillonite/chitosan gel	37:75	Enhanced removal of methyl orange on exfoliated montmorillonite/chitosan gel in presence of methylene blue.					
31524627	0	5	attach	removal	9:15	arg2	orange					27:32	methyl orange	20:32	methyl orange	20:32	Enhanced removal of methyl orange on exfoliated montmorillonite/chitosan gel in presence of methylene blue.					
31524627	2	6	used	used	492:495	arg2	techniques					324:333	Several techniques	316:333	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)	316:485	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	6	used	used	492:495	arg2	microscope					363:372	scanning electron microscope	345:372	scanning electron microscope (SEM)	345:378	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	0	7	theme	methylene	92:100	arg1	presence					80:87	presence	80:87	presence of methylene blue	80:105	Enhanced removal of methyl orange on exfoliated montmorillonite/chitosan gel in presence of methylene blue.					
31524627	2	8	theme	EMCG	529:532	arg1	characterization					505:520	the characterization	501:520	the characterization of the EMCG	501:532	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	5	9	dep	MB	872:873	arg1	presence					860:867	presence	860:867	presence	860:867	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	5	9	dep	MB	872:873	arg1	the					856:858	the	856:858	the	856:858	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	5	10	theme	adsorption	804:813	arg1	capacity					815:822	the maximum adsorption capacity	792:822	the maximum adsorption capacity of MO	792:828	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	6	11	theme	multifunctional	1253:1267	arg1	adsorbents					1269:1278	the multifunctional adsorbents	1249:1278	the multifunctional adsorbents	1249:1278	This finding of the synergistic effect between the two dyes on the proposed composite opens up new vistas to imagine the enhanced purification of the wastewater with multiple dyes co-existed using the multifunctional adsorbents.					
31524627	3	12	theme	cation	656:661	arg1	exchange					663:670	cation exchange	656:670	cation exchange	656:670	The EMCG performed well in the removal of MO attributing to the electrostatic attraction, cation exchange and hydrogen bond.					
31524627	6	13	theme	synergistic	1072:1082	arg1	effect					1084:1089	the synergistic effect	1068:1089	the synergistic effect between the two dyes on the proposed composite	1068:1136	This finding of the synergistic effect between the two dyes on the proposed composite opens up new vistas to imagine the enhanced purification of the wastewater with multiple dyes co-existed using the multifunctional adsorbents.					
31524627	1	14	theme	exfoliated	161:170	arg1	montmorillonite					172:186	exfoliated montmorillonite	161:186	exfoliated montmorillonite	161:186	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	2	15	theme	photoelectron	454:466	arg1	spectroscopy					468:479	X-ray photoelectron spectroscopy	448:479	X-ray photoelectron spectroscopy (XPS)	448:485	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	15	theme	photoelectron	454:466	arg1	XPS					482:484	XPS	482:484	XPS	482:484	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	3	16	theme	electrostatic	630:642	arg1	attraction					644:653	the electrostatic attraction	626:653	the electrostatic attraction	626:653	The EMCG performed well in the removal of MO attributing to the electrostatic attraction, cation exchange and hydrogen bond.					
31524627	2	17	theme	MO	562:563	arg1	removal					551:557	removal	551:557	removal of MO	551:563	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	4	18	theme	adsorption	695:704	arg1	behaviors					706:714	The adsorption behaviors	691:714	The adsorption behaviors	691:714	The adsorption behaviors were followed pseudo-second-order and Langmuir isotherm.					
31524627	2	19	theme	electron	354:361	arg1	fourier					397:403	fourier	397:403	fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)	397:485	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	19	theme	electron	354:361	arg1	SEM					375:377	SEM	375:377	SEM	375:377	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	19	theme	electron	354:361	arg1	microscope					363:372	scanning electron microscope	345:372	scanning electron microscope (SEM)	345:378	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	19	theme	electron	354:361	arg1	potential					386:394	zeta potential	381:394	zeta potential	381:394	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	20	dep	fourier	397:403	arg1	transform					405:413	transform	405:413	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)	405:485	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	6	21	from	effect	1084:1089	arg1	composite					1128:1136	the proposed composite	1115:1136	the proposed composite	1115:1136	This finding of the synergistic effect between the two dyes on the proposed composite opens up new vistas to imagine the enhanced purification of the wastewater with multiple dyes co-existed using the multifunctional adsorbents.					
31524627	2	22	theme	scanning	345:352	arg1	fourier					397:403	fourier	397:403	fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)	397:485	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	22	theme	scanning	345:352	arg1	SEM					375:377	SEM	375:377	SEM	375:377	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	22	theme	scanning	345:352	arg1	microscope					363:372	scanning electron microscope	345:372	scanning electron microscope (SEM)	345:378	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	22	theme	scanning	345:352	arg1	potential					386:394	zeta potential	381:394	zeta potential	381:394	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	3	23	theme	MO	608:609	arg1	removal					597:603	the removal	593:603	the removal of MO attributing to the electrostatic attraction, cation exchange and hydrogen bond	593:688	The EMCG performed well in the removal of MO attributing to the electrostatic attraction, cation exchange and hydrogen bond.					
31524627	3	24	theme	hydrogen	676:683	arg1	bond					685:688	hydrogen bond	676:688	hydrogen bond	676:688	The EMCG performed well in the removal of MO attributing to the electrostatic attraction, cation exchange and hydrogen bond.					
31524627	5	25	theme	MO	827:828	arg1	capacity					815:822	the maximum adsorption capacity	792:822	the maximum adsorption capacity of MO	792:828	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	0	26	theme	methyl	20:25	arg1	orange					27:32	methyl orange	20:32	methyl orange	20:32	Enhanced removal of methyl orange on exfoliated montmorillonite/chitosan gel in presence of methylene blue.					
31524627	5	27	from	effect	959:964	arg1	EMCG					975:978	EMCG	975:978	EMCG which allowed MO and MB to alternately arrange at the adsorption sites	975:1049	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	2	28	theme	zeta	381:384	arg1	microscope					363:372	scanning electron microscope	345:372	scanning electron microscope (SEM)	345:378	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	28	theme	zeta	381:384	arg1	potential					386:394	zeta potential	381:394	zeta potential	381:394	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	5	29	theme	shielding	949:957	arg1	effect					959:964	the shielding effect	945:964	the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites	945:1049	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	6	30	theme	proposed	1119:1126	arg1	composite					1128:1136	the proposed composite	1115:1136	the proposed composite	1115:1136	This finding of the synergistic effect between the two dyes on the proposed composite opens up new vistas to imagine the enhanced purification of the wastewater with multiple dyes co-existed using the multifunctional adsorbents.					
31524627	0	31	theme	orange	27:32	arg1	removal					9:15	removal	9:15	removal of methyl orange on exfoliated montmorillonite/chitosan gel	9:75	Enhanced removal of methyl orange on exfoliated montmorillonite/chitosan gel in presence of methylene blue.					
31524627	5	32	from	sites	1045:1049	arg1	arrange					1019:1025	arrange	1019:1025	arrange	1019:1025	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	4	33	theme	Langmuir	754:761	arg1	isotherm					763:770	Langmuir isotherm	754:770	Langmuir isotherm	754:770	The adsorption behaviors were followed pseudo-second-order and Langmuir isotherm.					
31524627	5	34	theme	maximum	796:802	arg1	capacity					815:822	the maximum adsorption capacity	792:822	the maximum adsorption capacity of MO	792:828	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	0	35	theme	montmorillonite/chitosan	48:71	arg1	gel					73:75	exfoliated montmorillonite/chitosan gel	37:75	exfoliated montmorillonite/chitosan gel	37:75	Enhanced removal of methyl orange on exfoliated montmorillonite/chitosan gel in presence of methylene blue.					
31524627	5	36	dep	arrange	1019:1025	arg1	to					1004:1005	to	1004:1005	to	1004:1005	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	1	37	attach	removing	229:236	arg1	water					262:266	water	262:266	water	262:266	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	1	37	attach	removing	229:236	arg2	gels					137:140	self-assembled gels	122:140	self-assembled gels	122:140	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	1	37	attach	removing	229:236	arg2	adsorbent					215:223	adsorbent	215:223	adsorbent	215:223	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	5	38	theme	adsorption	1034:1043	arg1	sites					1045:1049	the adsorption sites	1030:1049	the adsorption sites	1030:1049	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	0	39	theme	exfoliated	37:46	arg1	gel					73:75	exfoliated montmorillonite/chitosan gel	37:75	exfoliated montmorillonite/chitosan gel	37:75	Enhanced removal of methyl orange on exfoliated montmorillonite/chitosan gel in presence of methylene blue.					
31524627	6	40	theme	wastewater	1202:1211	arg1	purification					1182:1193	the enhanced purification	1169:1193	the enhanced purification of the wastewater with multiple dyes	1169:1230	This finding of the synergistic effect between the two dyes on the proposed composite opens up new vistas to imagine the enhanced purification of the wastewater with multiple dyes co-existed using the multifunctional adsorbents.					
31524627	5	41	theme	MB	969:970	arg1	effect					959:964	the shielding effect	945:964	the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites	945:1049	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	5	42	theme	MB	921:922	arg1	absence					910:916	absence	910:916	absence of MB	910:922	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	6	43	theme	new	1147:1149	arg1	vistas					1151:1156	new vistas	1147:1156	new vistas	1147:1156	This finding of the synergistic effect between the two dyes on the proposed composite opens up new vistas to imagine the enhanced purification of the wastewater with multiple dyes co-existed using the multifunctional adsorbents.					
31524627	6	44	theme	multiple	1218:1225	arg1	dyes					1227:1230	multiple dyes	1218:1230	multiple dyes	1218:1230	This finding of the synergistic effect between the two dyes on the proposed composite opens up new vistas to imagine the enhanced purification of the wastewater with multiple dyes co-existed using the multifunctional adsorbents.					
31524627	1	45	theme	self-assembled	122:135	arg1	adsorbent					215:223	adsorbent	215:223	adsorbent	215:223	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	1	45	theme	self-assembled	122:135	arg1	gels					137:140	self-assembled gels	122:140	self-assembled gels	122:140	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	6	46	with	purification	1182:1193	arg1	dyes					1227:1230	multiple dyes	1218:1230	multiple dyes	1218:1230	This finding of the synergistic effect between the two dyes on the proposed composite opens up new vistas to imagine the enhanced purification of the wastewater with multiple dyes co-existed using the multifunctional adsorbents.					
31524627	2	47	theme	X-ray	448:452	arg1	spectroscopy					468:479	X-ray photoelectron spectroscopy	448:479	X-ray photoelectron spectroscopy (XPS)	448:485	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	47	theme	X-ray	448:452	arg1	XPS					482:484	XPS	482:484	XPS	482:484	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	5	48	dep	increased	883:891	arg1	absence					910:916	absence	910:916	absence of MB	910:922	More surprisingly, the maximum adsorption capacity of MO was obviously enhanced in the presence of MB that it increased from 545 mg g-1 (absence of MB) to 1060 mg g-1 with the shielding effect of MB on EMCG which allowed MO and MB to alternately arrange at the adsorption sites.					
31524627	2	49	theme	infrared	415:422	arg1	FTIR					438:441	FTIR	438:441	FTIR	438:441	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	49	theme	infrared	415:422	arg1	spectroscopy					424:435	infrared spectroscopy	415:435	infrared spectroscopy (FTIR)	415:442	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	1	50	theme	methylene	295:303	arg1	presence					283:290	presence	283:290	presence	283:290	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	1	50	theme	methylene	295:303	arg1	absence					271:277	absence	271:277	absence	271:277	In this work, self-assembled gels were prepared with exfoliated montmorillonite and chitosan (EMCG) as the adsorbent for removing methyl orange (MO) from water in absence and presence of methylene blue (MB).					
31524627	2	51	theme	Several	316:322	arg1	microscope					363:372	scanning electron microscope	345:372	scanning electron microscope (SEM)	345:378	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
31524627	2	51	theme	Several	316:322	arg1	techniques					324:333	Several techniques	316:333	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)	316:485	Several techniques including scanning electron microscope (SEM), zeta potential, fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) were used for the characterization of the EMCG before and after removal of MO.					
30620405	2	0	from	regulation	424:433	arg1	beneficial					390:399	beneficial	390:399	beneficial	390:399	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	7	1	theme	raw	1123:1125	arg1	starch					1145:1150	raw unmodified potato starch	1123:1150	raw unmodified potato starch (resistant starch)	1123:1169	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	7	1	theme	raw	1123:1125	arg1	starch					1163:1168	resistant starch	1153:1168	resistant starch	1153:1168	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	13	2	theme	fractional	1953:1962	arg1	representation					1964:1977	increased fractional representation	1943:1977	increased fractional representation of Alistipes	1943:1990	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	10	3	theme	resistant	1491:1499	arg1	starch					1501:1506	resistant starch	1491:1506	resistant starch supplementation	1491:1522	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	16	4	theme	AAP	2272:2274	arg1	treatment					2276:2284	AAP treatment	2272:2284	AAP treatment	2272:2284	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	11	5	theme	multivariate	1608:1619	arg1	analysis					1621:1628	permutational multivariate analysis	1594:1628	permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively	1594:1694	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	9	6	theme	DNA	1339:1341	arg1	composition					1343:1353	gut microbiome DNA composition	1324:1353	gut microbiome DNA composition	1324:1353	The primary outcome was gut microbiome DNA composition.					
30620405	9	6	theme	DNA	1339:1341	arg1	outcome					1312:1318	The primary outcome	1300:1318	The primary outcome	1300:1318	The primary outcome was gut microbiome DNA composition.					
30620405	10	7	theme	Microbiome	1356:1365	arg1	DNA					1367:1369	Microbiome DNA	1356:1369	Microbiome DNA obtained from stool samples from all patients	1356:1415	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	8	8	theme	patients	1230:1237	arg1	MEASUREMENTS					1190:1201	MEASUREMENTS	1190:1201	MEASUREMENTS	1190:1201	MEASUREMENTS AND MAIN RESULTS Of the 37 patients, the mean ± SD age was 52.2 ± 12.5 years, and 57% were male.					
30620405	8	8	theme	patients	1230:1237	arg1	RESULTS					1212:1218	MAIN RESULTS	1207:1218	MAIN RESULTS	1207:1218	MEASUREMENTS AND MAIN RESULTS Of the 37 patients, the mean ± SD age was 52.2 ± 12.5 years, and 57% were male.					
30620405	6	9	with	adults	839:844	arg1	diagnosis					853:861	a diagnosis	851:861	a diagnosis of bipolar disorder or schizophrenia	851:898	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	15	10	theme	phylum	2216:2221	arg1	abundance					2184:2192	an increased abundance	2171:2192	an increased abundance of the Actinobacteria phylum	2171:2221	Although the microbiome of AAP-treated patients varied with resistant starch administration, an increased abundance of the Actinobacteria phylum was observed.					
30620405	6	11	theme	[21	993:995	arg1	patients					997:1004	ziprasidone [21 patients	981:1004	ziprasidone [21 patients	981:1004	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	12	12	theme	abundant	1712:1719	arg1	organisms					1721:1729	Differentially abundant organisms	1697:1729	Differentially abundant organisms	1697:1729	Differentially abundant organisms were detected by using linear discriminant analysis effect size.					
30620405	16	13	theme	species	2407:2413	arg1	richness					2415:2422	reduced species richness	2399:2422	reduced species richness	2399:2422	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	5	14	theme	SETTING	758:764	arg1	study					770:774	The study	766:774	SETTING The study	758:774	SETTING The study was performed in an outpatient setting.					
30620405	13	15	theme	significant	1808:1818	arg1	difference					1820:1829	no significant difference	1805:1829	no significant difference in overall microbiota composition	1805:1863	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	17	16	theme	starch	2560:2565	arg1	degraders					2567:2575	starch degraders	2560:2575	starch degraders	2560:2575	Additionally, variable microbiome responses to resistant starch supplementation were seen, with a significant increase in starch degraders.					
30620405	8	17	theme	±	1249:1249	arg1	patients					1230:1237	the 37 patients	1223:1237	the 37 patients	1223:1237	MEASUREMENTS AND MAIN RESULTS Of the 37 patients, the mean ± SD age was 52.2 ± 12.5 years, and 57% were male.					
30620405	8	17	theme	±	1249:1249	arg1	age					1254:1256	the mean ± SD age	1240:1256	the mean ± SD age	1240:1256	MEASUREMENTS AND MAIN RESULTS Of the 37 patients, the mean ± SD age was 52.2 ± 12.5 years, and 57% were male.					
30620405	3	18	theme	treatment	627:635	arg1	effects					612:618	the effects	608:618	the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population	608:718	This investigation was undertaken to delineate mechanistically the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population.					
30620405	1	19	theme	gut	229:231	arg1	microbiota					233:242	gut microbiota	229:242	gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden	229:336	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	17	20	theme	microbiome	2461:2470	arg1	responses					2472:2480	variable microbiome responses	2452:2480	variable microbiome responses to resistant starch supplementation	2452:2516	Additionally, variable microbiome responses to resistant starch supplementation were seen, with a significant increase in starch degraders.					
30620405	3	21	theme	supplementation	658:672	arg1	effects					612:618	the effects	608:618	the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population	608:718	This investigation was undertaken to delineate mechanistically the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population.					
30620405	0	22	from	Composition	99:109	arg1	Cohort					116:121	a Cohort	114:121	a Cohort of Patients with Bipolar Disorder or Schizophrenia	114:172	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	4	23	theme	Cross-sectional	728:742	arg1	study					751:755	Cross-sectional cohort study	728:755	DESIGN Cross-sectional cohort study.	721:756	DESIGN Cross-sectional cohort study.					
30620405	0	24	theme	Supplementation	65:79	arg1	Effects					0:6	Effects	0:6	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.	0:173	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	3	25	theme	resistant	641:649	arg1	supplementation					658:672	resistant starch supplementation	641:672	resistant starch supplementation	641:672	This investigation was undertaken to delineate mechanistically the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population.					
30620405	10	26	theme	stool	1385:1389	arg1	samples					1391:1397	stool samples	1385:1397	stool samples from all patients	1385:1415	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	16	27	theme	AAP-treated	2370:2380	arg1	patients					2382:2389	female AAP-treated patients	2363:2389	female AAP-treated patients in whom reduced species richness was observed	2363:2435	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	0	28	theme	Gut	84:86	arg1	Composition					99:109	Gut Microbiome Composition	84:109	Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia	84:172	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	0	29	from	Effects	0:6	arg1	Composition					99:109	Gut Microbiome Composition	84:109	Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia	84:172	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	13	30	theme	AAP	1898:1900	arg1	users					1902:1906	AAP users	1898:1906	AAP users	1898:1906	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	15	31	theme	AAP-treated	2105:2115	arg1	patients					2117:2124	AAP-treated patients	2105:2124	AAP-treated patients	2105:2124	Although the microbiome of AAP-treated patients varied with resistant starch administration, an increased abundance of the Actinobacteria phylum was observed.					
30620405	2	32	theme	AAP-treated	520:530	arg1	individuals					532:542	AAP-treated individuals	520:542	AAP-treated individuals	520:542	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	12	33	dep	analysis	1774:1781	arg1	size					1790:1793	effect size	1783:1793	linear discriminant analysis effect size	1754:1793	Differentially abundant organisms were detected by using linear discriminant analysis effect size.					
30620405	4	34	dep	DESIGN	721:726	arg1	study					751:755	Cross-sectional cohort study	728:755	DESIGN Cross-sectional cohort study.	721:756	DESIGN Cross-sectional cohort study.					
30620405	7	35	from	Patients	1088:1095	arg1	group					1108:1112	the AAP group	1100:1112	the AAP group	1100:1112	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	10	36	theme	16S	1432:1434	arg1	RNA					1446:1448	16S ribosomal RNA	1432:1448	16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation	1432:1522	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	10	36	theme	16S	1432:1434	arg1	rRNA					1451:1454	rRNA	1451:1454	rRNA	1451:1454	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	13	37	theme	Alistipes	1982:1990	arg1	representation					1964:1977	increased fractional representation	1943:1977	increased fractional representation of Alistipes	1943:1990	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	0	38	theme	Atypical	11:18	arg1	Treatment					34:42	Atypical Antipsychotic Treatment	11:42	Atypical Antipsychotic Treatment	11:42	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	16	39	theme	gut	2331:2333	arg1	microbiota					2335:2344	gut microbiota	2331:2344	gut microbiota	2331:2344	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	11	40	theme	Simpson	1658:1664	arg1	Index					1676:1680	the Inverse Simpson Diversity Index	1646:1680	the Inverse Simpson Diversity Index	1646:1680	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	13	41	theme	overall	1834:1840	arg1	composition					1853:1863	overall microbiota composition	1834:1863	overall microbiota composition	1834:1863	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	2	42	theme	glucose	416:422	arg1	regulation					424:433	glucose regulation	416:433	glucose regulation	416:433	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	0	43	theme	Treatment	34:42	arg1	Effects					0:6	Effects	0:6	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.	0:173	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	2	44	from	beneficial	390:399	arg1	obesity					404:410	obesity	404:410	obesity	404:410	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	2	44	from	beneficial	390:399	arg1	regulation					424:433	glucose regulation	416:433	glucose regulation	416:433	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	17	45	theme	starch	2495:2500	arg1	supplementation					2502:2516	resistant starch supplementation	2485:2516	resistant starch supplementation	2485:2516	Additionally, variable microbiome responses to resistant starch supplementation were seen, with a significant increase in starch degraders.					
30620405	3	46	theme	gut	677:679	arg1	microbiota					681:690	gut microbiota	677:690	gut microbiota in a psychiatric population	677:718	This investigation was undertaken to delineate mechanistically the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population.					
30620405	7	47	theme	potato	1138:1143	arg1	starch					1145:1150	raw unmodified potato starch	1123:1150	raw unmodified potato starch (resistant starch)	1123:1169	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	7	47	theme	potato	1138:1143	arg1	starch					1163:1168	resistant starch	1153:1168	resistant starch	1153:1168	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	1	48	theme	metabolic	321:329	arg1	burden					331:336	metabolic burden	321:336	metabolic burden	321:336	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	13	49	from	difference	1820:1829	arg1	composition					1853:1863	overall microbiota composition	1834:1863	overall microbiota composition	1834:1863	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	2	50	from	obesity	404:410	arg1	beneficial					390:399	beneficial	390:399	beneficial	390:399	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	10	51	theme	RNA	1446:1448	arg1	sequencing					1462:1471	16S ribosomal RNA (rRNA) gene sequencing	1432:1471	16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation	1432:1522	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	13	52	theme	non-AAP	1922:1928	arg1	users					1930:1934	non-AAP users	1922:1934	non-AAP users	1922:1934	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	2	53	theme	Dietary	339:345	arg1	prebiotics					347:356	Dietary prebiotics	339:356	Dietary prebiotics such as resistant starch	339:381	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	2	53	theme	Dietary	339:345	arg1	starch					376:381	resistant starch	366:381	resistant starch	366:381	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	6	54	theme	bipolar	866:872	arg1	disorder					874:881	bipolar disorder	866:881	bipolar disorder	866:881	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	15	55	theme	resistant	2138:2146	arg1	administration					2155:2168	resistant starch administration	2138:2168	resistant starch administration	2138:2168	Although the microbiome of AAP-treated patients varied with resistant starch administration, an increased abundance of the Actinobacteria phylum was observed.					
30620405	12	56	theme	discriminant	1761:1772	arg1	analysis					1774:1781	linear discriminant analysis	1754:1781	linear discriminant analysis effect size	1754:1793	Differentially abundant organisms were detected by using linear discriminant analysis effect size.					
30620405	3	57	theme	psychiatric	697:707	arg1	population					709:718	a psychiatric population	695:718	a psychiatric population	695:718	This investigation was undertaken to delineate mechanistically the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population.					
30620405	9	58	theme	gut	1324:1326	arg1	composition					1343:1353	gut microbiome DNA composition	1324:1353	gut microbiome DNA composition	1324:1353	The primary outcome was gut microbiome DNA composition.					
30620405	9	58	theme	gut	1324:1326	arg1	outcome					1312:1318	The primary outcome	1300:1318	The primary outcome	1300:1318	The primary outcome was gut microbiome DNA composition.					
30620405	0	59	theme	Starch	58:63	arg1	Supplementation					65:79	Resistant Starch Supplementation	48:79	Resistant Starch Supplementation	48:79	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	16	60	from	differences	2316:2326	arg1	patients					2382:2389	female AAP-treated patients	2363:2389	female AAP-treated patients in whom reduced species richness was observed	2363:2435	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	16	60	from	differences	2316:2326	arg1	microbiota					2335:2344	gut microbiota	2331:2344	gut microbiota	2331:2344	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	12	61	theme	effect	1783:1788	arg1	size					1790:1793	effect size	1783:1793	linear discriminant analysis effect size	1754:1793	Differentially abundant organisms were detected by using linear discriminant analysis effect size.					
30620405	6	62	dep	AAP	925:927	arg1	quetiapine					966:975	quetiapine	966:975	quetiapine	966:975	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	6	62	dep	AAP	925:927	arg1	risperidone					953:963	risperidone	953:963	risperidone	953:963	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	6	62	dep	AAP	925:927	arg1	olanzapine					941:950	olanzapine	941:950	olanzapine	941:950	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	6	62	dep	AAP	925:927	arg1	clozapine					930:938	clozapine	930:938	clozapine	930:938	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	6	62	dep	AAP	925:927	arg1	patients					997:1004	ziprasidone [21 patients	981:1004	ziprasidone [21 patients	981:1004	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	10	63	theme	gene	1457:1460	arg1	sequencing					1462:1471	16S ribosomal RNA (rRNA) gene sequencing	1432:1471	16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation	1432:1522	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	11	64	theme	permutational	1594:1606	arg1	analysis					1621:1628	permutational multivariate analysis	1594:1628	permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively	1594:1694	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	11	65	theme	microbial	1547:1555	arg1	diversity					1557:1565	intragroup microbial diversity	1536:1565	intragroup microbial diversity	1536:1565	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	2	66	theme	resistant	366:374	arg1	starch					376:381	resistant starch	366:381	resistant starch	366:381	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	1	67	theme	antipsychotic	269:281	arg1	treatment					289:297	atypical antipsychotic (AAP) treatment	260:297	atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden	260:336	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	13	68	located	detected	1869:1876	arg1	baseline					1881:1888	baseline	1881:1888	baseline	1881:1888	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	13	68	located	detected	1869:1876	arg2	difference					1820:1829	no significant difference	1805:1829	no significant difference in overall microbiota composition	1805:1863	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	6	69	dep	PATIENTS	816:823	arg1	total					827:831	A total	825:831	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.	816:1073	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	10	70	theme	starch	1501:1506	arg1	supplementation					1508:1522	resistant starch supplementation	1491:1522	resistant starch supplementation	1491:1522	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	7	71	theme	AAP	1104:1106	arg1	group					1108:1112	the AAP group	1100:1112	the AAP group	1100:1112	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	16	72	theme	reduced	2399:2405	arg1	richness					2415:2422	reduced species richness	2399:2422	reduced species richness	2399:2422	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	1	73	theme	AAP	284:286	arg1	treatment					289:297	atypical antipsychotic (AAP) treatment	260:297	atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden	260:336	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	3	74	from	microbiota	681:690	arg1	population					709:718	a psychiatric population	695:718	a psychiatric population	695:718	This investigation was undertaken to delineate mechanistically the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population.					
30620405	11	75	theme	variance	1633:1640	arg1	analysis					1621:1628	permutational multivariate analysis	1594:1628	permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively	1594:1694	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	6	76	theme	adults	839:844	arg1	total					827:831	A total	825:831	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.	816:1073	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	1	77	attach	link	308:311	arg2	treatment					289:297	atypical antipsychotic (AAP) treatment	260:297	atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden	260:336	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	1	77	attach	link	308:311	arg1	burden					331:336	metabolic burden	321:336	metabolic burden	321:336	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	15	78	theme	Actinobacteria	2201:2214	arg1	phylum					2216:2221	the Actinobacteria phylum	2197:2221	the Actinobacteria phylum	2197:2221	Although the microbiome of AAP-treated patients varied with resistant starch administration, an increased abundance of the Actinobacteria phylum was observed.					
30620405	1	79	theme	STUDY	175:179	arg1	OBJECTIVE					181:189	STUDY OBJECTIVE	175:189	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.	175:337	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	0	80	theme	Resistant	48:56	arg1	Supplementation					65:79	Resistant Starch Supplementation	48:79	Resistant Starch Supplementation	48:79	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	7	81	theme	INTERVENTION	1075:1086	arg1	Patients					1088:1095	INTERVENTION Patients	1075:1095	INTERVENTION Patients in the AAP group	1075:1112	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	1	82	theme	Previous	191:198	arg1	studies					200:206	Previous studies	191:206	Previous studies	191:206	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	17	83	theme	significant	2536:2546	arg1	increase					2548:2555	a significant increase	2534:2555	a significant increase in starch degraders	2534:2575	Additionally, variable microbiome responses to resistant starch supplementation were seen, with a significant increase in starch degraders.					
30620405	0	84	theme	Patients	126:133	arg1	Cohort					116:121	a Cohort	114:121	a Cohort of Patients with Bipolar Disorder or Schizophrenia	114:172	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	13	85	theme	increased	1943:1951	arg1	representation					1964:1977	increased fractional representation	1943:1977	increased fractional representation of Alistipes	1943:1990	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	6	86	theme	ziprasidone	981:991	arg1	patients					997:1004	ziprasidone [21 patients	981:1004	ziprasidone [21 patients	981:1004	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	16	87	located	observed	2428:2435	arg1	patients					2382:2389	female AAP-treated patients	2363:2389	female AAP-treated patients in whom reduced species richness was observed	2363:2435	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	16	87	located	observed	2428:2435	arg2	richness					2415:2422	reduced species richness	2399:2422	reduced species richness	2399:2422	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	0	88	theme	Bipolar	140:146	arg1	Disorder					148:155	Disorder	148:155	Disorder	148:155	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	3	89	theme	AAP	623:625	arg1	treatment					627:635	AAP treatment	623:635	AAP treatment	623:635	This investigation was undertaken to delineate mechanistically the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population.					
30620405	15	90	theme	increased	2174:2182	arg1	abundance					2184:2192	an increased abundance	2171:2192	an increased abundance of the Actinobacteria phylum	2171:2221	Although the microbiome of AAP-treated patients varied with resistant starch administration, an increased abundance of the Actinobacteria phylum was observed.					
30620405	2	91	from	microbiota	506:515	arg1	individuals					532:542	AAP-treated individuals	520:542	AAP-treated individuals	520:542	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	8	92	theme	mean	1244:1247	arg1	patients					1230:1237	the 37 patients	1223:1237	the 37 patients	1223:1237	MEASUREMENTS AND MAIN RESULTS Of the 37 patients, the mean ± SD age was 52.2 ± 12.5 years, and 57% were male.					
30620405	8	92	theme	mean	1244:1247	arg1	age					1254:1256	the mean ± SD age	1240:1256	the mean ± SD age	1240:1256	MEASUREMENTS AND MAIN RESULTS Of the 37 patients, the mean ± SD age was 52.2 ± 12.5 years, and 57% were male.					
30620405	8	93	theme	MAIN	1207:1210	arg1	RESULTS					1212:1218	MAIN RESULTS	1207:1218	MAIN RESULTS	1207:1218	MEASUREMENTS AND MAIN RESULTS Of the 37 patients, the mean ± SD age was 52.2 ± 12.5 years, and 57% were male.					
30620405	10	94	from	patients	1408:1415	arg1	samples					1391:1397	stool samples	1385:1397	stool samples from all patients	1385:1415	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	17	95	from	increase	2548:2555	arg1	degraders					2567:2575	starch degraders	2560:2575	starch degraders	2560:2575	Additionally, variable microbiome responses to resistant starch supplementation were seen, with a significant increase in starch degraders.					
30620405	8	96	theme	SD	1251:1252	arg1	patients					1230:1237	the 37 patients	1223:1237	the 37 patients	1223:1237	MEASUREMENTS AND MAIN RESULTS Of the 37 patients, the mean ± SD age was 52.2 ± 12.5 years, and 57% were male.					
30620405	8	96	theme	SD	1251:1252	arg1	age					1254:1256	the mean ± SD age	1240:1256	the mean ± SD age	1240:1256	MEASUREMENTS AND MAIN RESULTS Of the 37 patients, the mean ± SD age was 52.2 ± 12.5 years, and 57% were male.					
30620405	7	97	theme	resistant	1153:1161	arg1	starch					1145:1150	raw unmodified potato starch	1123:1150	raw unmodified potato starch (resistant starch)	1123:1169	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	7	97	theme	resistant	1153:1161	arg1	starch					1163:1168	resistant starch	1153:1168	resistant starch	1153:1168	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	3	98	theme	starch	651:656	arg1	supplementation					658:672	resistant starch supplementation	641:672	resistant starch supplementation	641:672	This investigation was undertaken to delineate mechanistically the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population.					
30620405	0	99	with	Patients	126:133	arg1	Schizophrenia					160:172	Schizophrenia	160:172	Schizophrenia	160:172	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	0	99	with	Patients	126:133	arg1	Disorder					148:155	Disorder	148:155	Disorder	148:155	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	16	100	theme	female	2363:2368	arg1	patients					2382:2389	female AAP-treated patients	2363:2389	female AAP-treated patients in whom reduced species richness was observed	2363:2435	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	5	101	theme	outpatient	796:805	arg1	setting					807:813	an outpatient setting	793:813	an outpatient setting	793:813	SETTING The study was performed in an outpatient setting.					
30620405	2	102	theme	gut	502:504	arg1	microbiota					506:515	gut microbiota	502:515	gut microbiota in AAP-treated individuals	502:542	Dietary prebiotics such as resistant starch may be beneficial in obesity and glucose regulation, but little is known mechanistically about their ability to modify gut microbiota in AAP-treated individuals.					
30620405	0	103	theme	Microbiome	88:97	arg1	Composition					99:109	Gut Microbiome Composition	84:109	Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia	84:172	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	14	104	theme	non-AAP-treated	2055:2069	arg1	women					2071:2075	non-AAP-treated women	2055:2075	non-AAP-treated women	2055:2075	AAP-treated women exhibited decreased diversity compared with non-AAP-treated women.					
30620405	4	105	theme	cohort	744:749	arg1	study					751:755	Cross-sectional cohort study	728:755	DESIGN Cross-sectional cohort study.	721:756	DESIGN Cross-sectional cohort study.					
30620405	13	106	theme	microbiota	1842:1851	arg1	composition					1853:1863	overall microbiota composition	1834:1863	overall microbiota composition	1834:1863	Although no significant difference in overall microbiota composition was detected at baseline between AAP users and nonusers, non-AAP users showed increased fractional representation of Alistipes.					
30620405	16	107	dep	CONCLUSION	2237:2246	arg1	suggest					2259:2265	suggest	2259:2265	suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed	2259:2435	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	16	108	theme	measurable	2305:2314	arg1	differences					2316:2326	measurable differences	2305:2326	measurable differences	2305:2326	CONCLUSION These data suggest that AAP treatment is associated with measurable differences in gut microbiota, particularly in female AAP-treated patients in whom reduced species richness was observed.					
30620405	17	109	theme	variable	2452:2459	arg1	responses					2472:2480	variable microbiome responses	2452:2480	variable microbiome responses to resistant starch supplementation	2452:2516	Additionally, variable microbiome responses to resistant starch supplementation were seen, with a significant increase in starch degraders.					
30620405	11	110	theme	Index	1676:1680	arg1	analysis					1621:1628	permutational multivariate analysis	1594:1628	permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively	1594:1694	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	3	111	from	effects	612:618	arg1	microbiota					681:690	gut microbiota	677:690	gut microbiota in a psychiatric population	677:718	This investigation was undertaken to delineate mechanistically the effects of AAP treatment and resistant starch supplementation on gut microbiota in a psychiatric population.					
30620405	0	112	theme	Antipsychotic	20:32	arg1	Treatment					34:42	Atypical Antipsychotic Treatment	11:42	Atypical Antipsychotic Treatment	11:42	Effects of Atypical Antipsychotic Treatment and Resistant Starch Supplementation on Gut Microbiome Composition in a Cohort of Patients with Bipolar Disorder or Schizophrenia.					
30620405	14	113	theme	AAP-treated	1993:2003	arg1	women					2005:2009	AAP-treated women	1993:2009	AAP-treated women	1993:2009	AAP-treated women exhibited decreased diversity compared with non-AAP-treated women.					
30620405	11	114	theme	Inverse	1650:1656	arg1	Index					1676:1680	the Inverse Simpson Diversity Index	1646:1680	the Inverse Simpson Diversity Index	1646:1680	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	17	115	theme	resistant	2485:2493	arg1	supplementation					2502:2516	resistant starch supplementation	2485:2516	resistant starch supplementation	2485:2516	Additionally, variable microbiome responses to resistant starch supplementation were seen, with a significant increase in starch degraders.					
30620405	11	116	theme	Inter-	1525:1530	arg1	measures					1567:1574	Inter- and intragroup microbial diversity measures	1525:1574	Inter- and intragroup microbial diversity measures	1525:1574	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	7	117	theme	unmodified	1127:1136	arg1	starch					1145:1150	raw unmodified potato starch	1123:1150	raw unmodified potato starch (resistant starch)	1123:1169	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	7	117	theme	unmodified	1127:1136	arg1	starch					1163:1168	resistant starch	1153:1168	resistant starch	1153:1168	INTERVENTION Patients in the AAP group received raw unmodified potato starch (resistant starch) daily for 14 days.					
30620405	6	118	theme	schizophrenia	886:898	arg1	diagnosis					853:861	a diagnosis	851:861	a diagnosis of bipolar disorder or schizophrenia	851:898	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	15	119	theme	starch	2148:2153	arg1	administration					2155:2168	resistant starch administration	2138:2168	resistant starch administration	2138:2168	Although the microbiome of AAP-treated patients varied with resistant starch administration, an increased abundance of the Actinobacteria phylum was observed.					
30620405	14	120	theme	decreased	2021:2029	arg1	diversity					2031:2039	decreased diversity	2021:2039	decreased diversity	2021:2039	AAP-treated women exhibited decreased diversity compared with non-AAP-treated women.					
30620405	1	121	dep	OBJECTIVE	181:189	arg1	identified					208:217	identified	208:217	identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden	208:336	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	9	122	theme	primary	1304:1310	arg1	composition					1343:1353	gut microbiome DNA composition	1324:1353	gut microbiome DNA composition	1324:1353	The primary outcome was gut microbiome DNA composition.					
30620405	9	122	theme	primary	1304:1310	arg1	outcome					1312:1318	The primary outcome	1300:1318	The primary outcome	1300:1318	The primary outcome was gut microbiome DNA composition.					
30620405	6	123	theme	disorder	874:881	arg1	diagnosis					853:861	a diagnosis	851:861	a diagnosis of bipolar disorder or schizophrenia	851:898	PATIENTS A total of 37 adults with a diagnosis of bipolar disorder or schizophrenia who were treated with an AAP (clozapine, olanzapine, risperidone, quetiapine, or ziprasidone [21 patients]) or lithium and/or lamotrigine (16 patients) for at least 6 months.					
30620405	10	124	theme	ribosomal	1436:1444	arg1	RNA					1446:1448	16S ribosomal RNA	1432:1448	16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation	1432:1522	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	10	124	theme	ribosomal	1436:1444	arg1	rRNA					1451:1454	rRNA	1451:1454	rRNA	1451:1454	Microbiome DNA obtained from stool samples from all patients was subject to 16S ribosomal RNA (rRNA) gene sequencing before and during resistant starch supplementation.					
30620405	11	125	theme	Diversity	1666:1674	arg1	Index					1676:1680	the Inverse Simpson Diversity Index	1646:1680	the Inverse Simpson Diversity Index	1646:1680	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	11	126	theme	diversity	1557:1565	arg1	measures					1567:1574	Inter- and intragroup microbial diversity measures	1525:1574	Inter- and intragroup microbial diversity measures	1525:1574	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	15	127	theme	patients	2117:2124	arg1	microbiome					2091:2100	the microbiome	2087:2100	the microbiome of AAP-treated patients	2087:2124	Although the microbiome of AAP-treated patients varied with resistant starch administration, an increased abundance of the Actinobacteria phylum was observed.					
30620405	12	128	theme	linear	1754:1759	arg1	analysis					1774:1781	linear discriminant analysis	1754:1781	linear discriminant analysis effect size	1754:1793	Differentially abundant organisms were detected by using linear discriminant analysis effect size.					
30620405	1	129	theme	atypical	260:267	arg1	treatment					289:297	atypical antipsychotic (AAP) treatment	260:297	atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden	260:336	STUDY OBJECTIVE Previous studies identified shifts in gut microbiota associated with atypical antipsychotic (AAP) treatment that may link AAPs to metabolic burden.					
30620405	11	130	theme	intragroup	1536:1545	arg1	diversity					1557:1565	intragroup microbial diversity	1536:1565	intragroup microbial diversity	1536:1565	Inter- and intragroup microbial diversity measures were performed by permutational multivariate analysis of variance and the Inverse Simpson Diversity Index, respectively.					
30620405	9	131	theme	microbiome	1328:1337	arg1	composition					1343:1353	gut microbiome DNA composition	1324:1353	gut microbiome DNA composition	1324:1353	The primary outcome was gut microbiome DNA composition.					
30620405	9	131	theme	microbiome	1328:1337	arg1	outcome					1312:1318	The primary outcome	1300:1318	The primary outcome	1300:1318	The primary outcome was gut microbiome DNA composition.					
31190796	6	0	theme	enhanced	1578:1585	arg1	expression					1587:1596	the enhanced expression	1574:1596	the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA)	1574:1681	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	3	1	theme	composite	842:850	arg1	membrane					852:859	the composite membrane	838:859	the composite membrane	838:859	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	6	2	theme	marker	1669:1674	arg1	expression					1587:1596	the enhanced expression	1574:1596	the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA)	1574:1681	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	3	3	dep	hydrophilicity	791:804	arg1	the					779:781	the	779:781	the	779:781	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	3	3	dep	hydrophilicity	791:804	arg1	ability					827:833	ability	827:833	ability	827:833	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	5	4	theme	human	1316:1320	arg1	cells					1349:1353	human umbilical vein endothelial cells	1316:1353	human umbilical vein endothelial cells	1316:1353	Additionally, it promoted the proliferation of keratinocytes and human umbilical vein endothelial cells and increased the secretion of VEGF.					
31190796	4	5	theme	composite	1003:1011	arg1	membrane					1013:1020	the composite membrane	999:1020	the composite membrane	999:1020	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	6	6	theme	proliferation	1655:1667	arg1	marker					1669:1674	keratinocyte proliferation marker	1642:1674	keratinocyte proliferation marker (PCNA)	1642:1681	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	6	6	theme	proliferation	1655:1667	arg1	PCNA					1677:1680	PCNA	1677:1680	PCNA	1677:1680	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	2	7	theme	eco-friendly	642:653	arg1	manner					655:660	an eco-friendly manner	639:660	an eco-friendly manner	639:660	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	5	8	theme	umbilical	1322:1330	arg1	cells					1349:1353	human umbilical vein endothelial cells	1316:1353	human umbilical vein endothelial cells	1316:1353	Additionally, it promoted the proliferation of keratinocytes and human umbilical vein endothelial cells and increased the secretion of VEGF.					
31190796	4	9	theme	excellent	1026:1034	arg1	activity					1050:1057	excellent antibacterial activity	1026:1057	excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli	1026:1188	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	4	10	theme	vitrobiological	961:975	arg1	results					977:983	the in vitrobiological results	954:983	the in vitrobiological results	954:983	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	6	11	theme	keratinocyte	1642:1653	arg1	marker					1669:1674	keratinocyte proliferation marker	1642:1674	keratinocyte proliferation marker (PCNA)	1642:1681	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	6	11	theme	keratinocyte	1642:1653	arg1	PCNA					1677:1680	PCNA	1677:1680	PCNA	1677:1680	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	7	12	theme	composite	1743:1751	arg1	candidate					1777:1785	a potential candidate	1765:1785	a potential candidate of wound dressings	1765:1804	Conclusion: These results indicated that the composite membrane is a potential candidate of wound dressings.					
31190796	7	12	theme	composite	1743:1751	arg1	membrane					1753:1760	the composite membrane	1739:1760	the composite membrane	1739:1760	Conclusion: These results indicated that the composite membrane is a potential candidate of wound dressings.					
31190796	0	13	theme	multi-drug-resistant	112:131	arg1	bacteria					133:140	multi-drug-resistant bacteria	112:140	multi-drug-resistant bacteria	112:140	Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane effectively kills multi-drug-resistant bacteria, promotes angiogenesis and accelerates re-epithelialization.					
31190796	6	14	theme	composite	1444:1452	arg1	membrane					1454:1461	the composite membrane	1440:1461	the composite membrane	1440:1461	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	3	15	theme	physicochemical	667:681	arg1	properties					683:692	The physicochemical properties	663:692	The physicochemical properties of the composite membrane	663:718	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	7	16	theme	dressings	1796:1804	arg1	candidate					1777:1785	a potential candidate	1765:1785	a potential candidate of wound dressings	1765:1804	Conclusion: These results indicated that the composite membrane is a potential candidate of wound dressings.					
31190796	7	16	theme	dressings	1796:1804	arg1	membrane					1753:1760	the composite membrane	1739:1760	the composite membrane	1739:1760	Conclusion: These results indicated that the composite membrane is a potential candidate of wound dressings.					
31190796	2	17	theme	biomimetic	489:498	arg1	nano/microfibres					540:555	biomimetic polydopamine-modified eggshell membrane nano/microfibres	489:555	biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA)	489:620	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	6	18	theme	angiogenetic	1601:1612	arg1	VEGF					1632:1635	VEGF	1632:1635	VEGF	1632:1635	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	6	18	theme	angiogenetic	1601:1612	arg1	CD31					1623:1626	CD31	1623:1626	CD31	1623:1626	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	6	18	theme	angiogenetic	1601:1612	arg1	markers					1614:1620	angiogenetic markers	1601:1620	angiogenetic markers (CD31 and VEGF)	1601:1636	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	3	19	theme	membrane	852:859	arg1	hydrophilicity					791:804	surface hydrophilicity	783:804	surface hydrophilicity	783:804	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	3	19	theme	membrane	852:859	arg1	absorption					816:825	water absorption	810:825	water absorption	810:825	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	5	20	theme	VEGF	1386:1389	arg1	secretion					1373:1381	the secretion	1369:1381	the secretion of VEGF	1369:1389	Additionally, it promoted the proliferation of keratinocytes and human umbilical vein endothelial cells and increased the secretion of VEGF.					
31190796	1	21	theme	wound	227:231	arg1	dressing					233:240	a wound dressing	225:240	a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization	225:358	Background: Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management.					
31190796	2	22	theme	antimicrobial	575:587	arg1	peptide					589:595	KR-12 antimicrobial peptide	569:595	KR-12 antimicrobial peptide	569:595	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	3	23	theme	successive	885:894	arg1	conjugation					896:906	the successive conjugation	881:906	the successive conjugation of the HA and the KR-12 peptide	881:938	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	6	24	theme	markers	1614:1620	arg1	expression					1587:1596	the enhanced expression	1574:1596	the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA)	1574:1681	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	2	25	theme	KR-12	569:573	arg1	peptide					589:595	KR-12 antimicrobial peptide	569:595	KR-12 antimicrobial peptide	569:595	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	1	26	theme	great	366:370	arg1	Background					203:212	Background	203:212	Background: Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management.	203:405	Background: Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management.					
31190796	1	26	theme	great	366:370	arg1	significance					372:383	great significance	366:383	great significance for wound management	366:404	Background: Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management.					
31190796	3	27	theme	composite	701:709	arg1	membrane					711:718	the composite membrane	697:718	the composite membrane	697:718	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	2	28	theme	composite	459:467	arg1	membrane					469:476	a biocompatible composite membrane	443:476	a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA)	443:620	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	0	29	theme	peptide-containing	21:38	arg1	acid					51:54	peptide-containing hyaluronic acid	21:54	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane effectively kills multi-drug-resistant bacteria, promotes angiogenesis and accelerates re-epithelialization.					
31190796	6	30	dep	markers	1614:1620	arg1	VEGF					1632:1635	VEGF	1632:1635	VEGF	1632:1635	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	6	30	dep	markers	1614:1620	arg1	CD31					1623:1626	CD31	1623:1626	CD31	1623:1626	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	6	30	dep	markers	1614:1620	arg1	markers					1614:1620	angiogenetic markers	1601:1620	angiogenetic markers (CD31 and VEGF)	1601:1636	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	3	31	theme	surface	783:789	arg1	hydrophilicity					791:804	surface hydrophilicity	783:804	surface hydrophilicity	783:804	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	6	32	dep	in	1404:1405	arg1	vivo					1407:1410	vivo	1407:1410	vivo	1407:1410	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	4	33	theme	MRSA	1212:1215	arg1	formation					1225:1233	MRSA biofilm formation	1212:1233	MRSA biofilm formation	1212:1233	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	2	34	theme	membrane	531:538	arg1	nano/microfibres					540:555	biomimetic polydopamine-modified eggshell membrane nano/microfibres	489:555	biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA)	489:620	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	0	35	theme	acid	51:54	arg1	membrane					85:92	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane effectively kills multi-drug-resistant bacteria, promotes angiogenesis and accelerates re-epithelialization.					
31190796	1	36	theme	wound	389:393	arg1	management					395:404	wound management	389:404	wound management	389:404	Background: Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management.					
31190796	6	37	theme	in	1404:1405	arg1	study					1419:1423	an in vivo animal study	1401:1423	an in vivo animal study	1401:1423	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	1	38	theme	multi-drug-resistant	268:287	arg1	infection					299:307	multi-drug-resistant bacterial infection	268:307	multi-drug-resistant bacterial infection	268:307	Background: Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management.					
31190796	6	39	theme	animal	1412:1417	arg1	study					1419:1423	an in vivo animal study	1401:1423	an in vivo animal study	1401:1423	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	2	40	theme	eggshell	522:529	arg1	nano/microfibres					540:555	biomimetic polydopamine-modified eggshell membrane nano/microfibres	489:555	biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA)	489:620	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	0	41	theme	hyaluronic	40:49	arg1	acid					51:54	peptide-containing hyaluronic acid	21:54	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane effectively kills multi-drug-resistant bacteria, promotes angiogenesis and accelerates re-epithelialization.					
31190796	5	42	theme	vein	1332:1335	arg1	cells					1349:1353	human umbilical vein endothelial cells	1316:1353	human umbilical vein endothelial cells	1316:1353	Additionally, it promoted the proliferation of keratinocytes and human umbilical vein endothelial cells and increased the secretion of VEGF.					
31190796	2	43	theme	hyaluronic	601:610	arg1	HA					618:619	HA	618:619	HA	618:619	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	2	43	theme	hyaluronic	601:610	arg1	acid					612:615	hyaluronic acid	601:615	hyaluronic acid (HA)	601:620	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	4	44	theme	biofilm	1217:1223	arg1	formation					1225:1233	MRSA biofilm formation	1212:1233	MRSA biofilm formation	1212:1233	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	1	45	theme	bacterial	289:297	arg1	infection					299:307	multi-drug-resistant bacterial infection	268:307	multi-drug-resistant bacterial infection	268:307	Background: Designing a wound dressing that effectively prevents multi-drug-resistant bacterial infection and promotes angiogenesis and re-epithelialization is of great significance for wound management.					
31190796	0	46	theme	fibrous	68:74	arg1	membrane					85:92	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane effectively kills multi-drug-resistant bacteria, promotes angiogenesis and accelerates re-epithelialization.					
31190796	5	47	theme	endothelial	1337:1347	arg1	cells					1349:1353	human umbilical vein endothelial cells	1316:1353	human umbilical vein endothelial cells	1316:1353	Additionally, it promoted the proliferation of keratinocytes and human umbilical vein endothelial cells and increased the secretion of VEGF.					
31190796	4	48	dep	Escherichia	1173:1183	arg1	coli					1185:1188	coli	1185:1188	coli	1185:1188	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	3	49	theme	membrane	711:718	arg1	properties					683:692	The physicochemical properties	663:692	The physicochemical properties of the composite membrane	663:718	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	0	50	theme	immobilized	56:66	arg1	membrane					85:92	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane effectively kills multi-drug-resistant bacteria, promotes angiogenesis and accelerates re-epithelialization.					
31190796	5	51	theme	cells	1349:1353	arg1	proliferation					1281:1293	the proliferation	1277:1293	the proliferation of keratinocytes and human umbilical vein endothelial cells	1277:1353	Additionally, it promoted the proliferation of keratinocytes and human umbilical vein endothelial cells and increased the secretion of VEGF.					
31190796	4	52	theme	antibacterial	1036:1048	arg1	activity					1050:1057	excellent antibacterial activity	1026:1057	excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli	1026:1188	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	3	53	theme	water	810:814	arg1	absorption					816:825	water absorption	810:825	water absorption	810:825	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	4	54	theme	in	958:959	arg1	results					977:983	the in vitrobiological results	954:983	the in vitrobiological results	954:983	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	3	55	theme	HA	915:916	arg1	conjugation					896:906	the successive conjugation	881:906	the successive conjugation of the HA and the KR-12 peptide	881:938	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	7	56	theme	potential	1767:1775	arg1	candidate					1777:1785	a potential candidate	1765:1785	a potential candidate of wound dressings	1765:1804	Conclusion: These results indicated that the composite membrane is a potential candidate of wound dressings.					
31190796	7	56	theme	potential	1767:1775	arg1	membrane					1753:1760	the composite membrane	1739:1760	the composite membrane	1739:1760	Conclusion: These results indicated that the composite membrane is a potential candidate of wound dressings.					
31190796	6	57	theme	wound	1477:1481	arg1	healing					1483:1489	wound healing	1477:1489	wound healing	1477:1489	Finally, an in vivo animal study indicated that the composite membrane could promote wound healing via accelerating angiogenesis and re-epithelialization, which were demonstrated by the enhanced expression of angiogenetic markers (CD31 and VEGF) and keratinocyte proliferation marker (PCNA), respectively.					
31190796	2	58	dep	Methods	407:413	arg1	developed					626:634	developed	626:634	was developed in an eco-friendly manner	622:660	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	7	59	theme	wound	1790:1794	arg1	dressings					1796:1804	wound dressings	1790:1804	wound dressings	1790:1804	Conclusion: These results indicated that the composite membrane is a potential candidate of wound dressings.					
31190796	0	60	theme	membrane	85:92	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	0:92	Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane effectively kills multi-drug-resistant bacteria, promotes angiogenesis and accelerates re-epithelialization.					
31190796	2	61	theme	polydopamine-modified	500:520	arg1	nano/microfibres					540:555	biomimetic polydopamine-modified eggshell membrane nano/microfibres	489:555	biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA)	489:620	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	2	62	theme	biocompatible	445:457	arg1	membrane					469:476	a biocompatible composite membrane	443:476	a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA)	443:620	Methods and results: In this study, a biocompatible composite membrane comprising biomimetic polydopamine-modified eggshell membrane nano/microfibres coated with KR-12 antimicrobial peptide and hyaluronic acid (HA) was developed in an eco-friendly manner.					
31190796	3	63	theme	KR-12	926:930	arg1	peptide					932:938	the HA and the KR-12 peptide	911:938	peptide	932:938	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
31190796	0	64	theme	eggshell	76:83	arg1	membrane					85:92	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane	15:92	Fabrication of KR-12 peptide-containing hyaluronic acid immobilized fibrous eggshell membrane effectively kills multi-drug-resistant bacteria, promotes angiogenesis and accelerates re-epithelialization.					
31190796	5	65	theme	keratinocytes	1298:1310	arg1	proliferation					1281:1293	the proliferation	1277:1293	the proliferation of keratinocytes and human umbilical vein endothelial cells	1277:1353	Additionally, it promoted the proliferation of keratinocytes and human umbilical vein endothelial cells and increased the secretion of VEGF.					
31190796	4	66	contain	had	1022:1024	arg1	membrane					1013:1020	the composite membrane	999:1020	the composite membrane	999:1020	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	4	66	contain	had	1022:1024	arg2	activity					1050:1057	excellent antibacterial activity	1026:1057	excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli	1026:1188	Furthermore, the in vitrobiological results revealed that the composite membrane had excellent antibacterial activity against Gram-positive Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative Escherichia coli, and it could prevent MRSA biofilm formation on its surface.					
31190796	3	67	theme	peptide	932:938	arg1	conjugation					896:906	the successive conjugation	881:906	the successive conjugation of the HA and the KR-12 peptide	881:938	The physicochemical properties of the composite membrane were thoroughly characterized, and the results showed that the surface hydrophilicity and water absorption ability of the composite membrane were improved after the successive conjugation of the HA and the KR-12 peptide.					
29428761	4	0	theme	milk	717:720	arg1	SCS					742:744	SCS	742:744	SCS	742:744	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	4	0	theme	milk	717:720	arg1	score					735:739	milk somatic cell score	717:739	milk somatic cell score (SCS)	717:745	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	3	1	theme	cell	438:441	arg1	count					443:447	somatic cell count	430:447	somatic cell count	430:447	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	9	2	theme	Milk	1357:1360	arg1	samples					1362:1368	Milk samples	1357:1368	Milk samples with pH values lower than 6.56 versus higher than 6.78	1357:1423	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	11	3	theme	curd	1985:1988	arg1	firming					1990:1996	the curd firming	1981:1996	the curd firming over time equation	1981:2015	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	4	4	theme	cell	730:733	arg1	SCS					742:744	SCS	742:744	SCS	742:744	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	4	4	theme	cell	730:733	arg1	score					735:739	milk somatic cell score	717:739	milk somatic cell score (SCS)	717:745	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	6	5	theme	view	969:972	arg1	point					960:964	a phenotypic point	947:964	a phenotypic point of view	947:972	From a phenotypic point of view, higher SCS levels caused a delayed gelification of milk.					
29428761	13	6	theme	present	2179:2185	arg1	study					2187:2191	the present study	2175:2191	the present study	2175:2191	In conclusion, results reported in the present study suggest that SCS, pH, and lactose affect, contemporarily and independently, milk quality and MCP.					
29428761	3	7	theme	model	568:572	arg1	parameters					574:583	CF model parameters	565:583	CF model parameters	565:583	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	14	8	theme	milk	2487:2490	arg1	ability					2470:2476	cheese-making ability	2456:2476	cheese-making ability of ovine milk	2456:2490	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	1	9	theme	pH	237:238	arg1	effect					194:199	the effect	190:199	the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters	190:328	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	3	10	theme	point	457:461	arg1	MCP					463:465	single point MCP	450:465	single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60)	450:558	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	4	11	theme	levels	707:712	arg1	effects					682:688	the effects	678:688	the effects of the different levels of milk somatic cell score (SCS), lactose, and pH	678:762	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	14	12	theme	infrared	2368:2375	arg1	spectra					2377:2383	infrared spectra	2368:2383	infrared spectra prediction	2368:2394	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	9	13	theme	firmness	1555:1562	arg1	traits					1564:1569	the 3 curd firmness traits	1544:1569	all the 3 curd firmness traits	1540:1569	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	10	14	theme	view	1596:1599	arg1	point					1587:1591	a genetic point	1577:1591	a genetic point of view	1577:1599	From a genetic point of view, the marginal posterior distribution of heritability estimates evidenced a large and exploitable variability for all 3 phenotypes.					
29428761	14	15	theme	cheese-making	2456:2468	arg1	ability					2470:2476	cheese-making ability	2456:2476	cheese-making ability of ovine milk	2456:2490	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	4	16	theme	lactose	748:754	arg1	levels					707:712	the different levels	693:712	the different levels of milk somatic cell score (SCS), lactose, and pH	693:762	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	3	17	theme	coagulation	475:485	arg1	time					487:490	rennet coagulation time	468:490	rennet coagulation time	468:490	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	7	18	theme	coagulation	1143:1153	arg1	times					1155:1159	coagulation times	1143:1159	coagulation times	1143:1159	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	5	19	with	herd	797:800	arg1	composition					865:875	milk composition	860:875	milk composition	860:875	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	10	20	theme	posterior	1615:1623	arg1	distribution					1625:1636	the marginal posterior distribution	1602:1636	the marginal posterior distribution of heritability estimates	1602:1662	From a genetic point of view, the marginal posterior distribution of heritability estimates evidenced a large and exploitable variability for all 3 phenotypes.					
29428761	9	21	with	samples	1362:1368	arg1	values					1378:1383	pH values	1375:1383	pH values lower than 6.56 versus higher than 6.78	1375:1423	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	3	22	theme	firming	503:509	arg1	time					511:514	curd firming time	498:514	curd firming time	498:514	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	1	23	from	effect	194:199	arg1	MCP					291:293	MCP	291:293	MCP	291:293	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	1	23	from	effect	194:199	arg1	composition					254:264	sheep milk composition	243:264	sheep milk composition	243:264	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	1	23	from	effect	194:199	arg1	properties					279:288	coagulation properties	267:288	coagulation properties (MCP)	267:294	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	1	23	from	effect	194:199	arg1	parameters					319:328	curd firming (CF) parameters	301:328	curd firming (CF) parameters	301:328	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	9	24	dep	14.3	1480:1483	arg1	to					1477:1478	to	1477:1478	to	1477:1478	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	11	25	theme	mean	1736:1739	arg1	0.173					1780:1784	0.173	1780:1784	0.173	1780:1784	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	11	25	theme	mean	1736:1739	arg1	estimates					1765:1773	The mean intra-farm heritability estimates	1732:1773	The mean intra-farm heritability estimates	1732:1773	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	0	26	from	composition	100:110	arg1	sheep					152:156	dairy sheep	146:156	dairy sheep	146:156	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	7	27	theme	intense	1120:1126	arg1	effect					1128:1133	a very intense effect	1113:1133	a very intense effect on both coagulation times and curd firming	1113:1176	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	10	28	theme	estimates	1654:1662	arg1	distribution					1625:1636	the marginal posterior distribution	1602:1636	the marginal posterior distribution of heritability estimates	1602:1662	From a genetic point of view, the marginal posterior distribution of heritability estimates evidenced a large and exploitable variability for all 3 phenotypes.					
29428761	0	29	theme	dairy	146:150	arg1	sheep					152:156	dairy sheep	146:156	dairy sheep	146:156	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	11	30	theme	intra-farm	1741:1750	arg1	0.173					1780:1784	0.173	1780:1784	0.173	1780:1784	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	11	30	theme	intra-farm	1741:1750	arg1	estimates					1765:1773	The mean intra-farm heritability estimates	1732:1773	The mean intra-farm heritability estimates	1732:1773	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	3	31	dep	MCP	463:465	arg1	firmness					531:538	curd firmness	526:538	curd firmness	526:538	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	3	31	dep	MCP	463:465	arg1	time					487:490	rennet coagulation time	468:490	rennet coagulation time	468:490	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	3	31	dep	MCP	463:465	arg1	RCT					493:495	RCT	493:495	RCT	493:495	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	3	31	dep	MCP	463:465	arg1	k20					517:519	k20	517:519	k20	517:519	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	3	31	dep	MCP	463:465	arg1	time					511:514	curd firming time	498:514	curd firming time	498:514	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	6	32	theme	higher	975:980	arg1	levels					986:991	higher SCS levels	975:991	higher SCS levels	975:991	From a phenotypic point of view, higher SCS levels caused a delayed gelification of milk.					
29428761	0	33	theme	mammary	63:69	arg1	gland					71:75	the mammary gland	59:75	the mammary gland health status	59:89	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	11	34	theme	heritability	1752:1763	arg1	0.173					1780:1784	0.173	1780:1784	0.173	1780:1784	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	11	34	theme	heritability	1752:1763	arg1	estimates					1765:1773	The mean intra-farm heritability estimates	1732:1773	The mean intra-farm heritability estimates	1732:1773	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	9	35	theme	RCT	1462:1464	arg1	decrease					1528:1535	a decrease	1526:1535	a decrease of all the 3 curd firmness traits	1526:1569	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	35	theme	RCT	1462:1464	arg1	increase					1450:1457	an increase	1447:1457	an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min)	1447:1520	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	8	36	theme	time	1240:1243	arg1	equation					1245:1252	time equation	1240:1252	time equation	1240:1252	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	11	37	from	levels	1917:1922	arg1	pH					1873:1874	pH	1873:1874	pH (unfavorably)	1873:1888	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	11	37	from	levels	1917:1922	arg1	SCS					1865:1867	SCS	1865:1867	SCS	1865:1867	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	11	37	from	levels	1917:1922	arg1	unfavorably					1877:1887	unfavorably	1877:1887	unfavorably	1877:1887	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	0	38	theme	health	77:82	arg1	status					84:89	the mammary gland health status	59:89	the mammary gland health status	59:89	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	9	39	theme	pH	1375:1376	arg1	values					1378:1383	pH values	1375:1383	pH values lower than 6.56 versus higher than 6.78	1375:1423	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	11	40	theme	curd	2045:2048	arg1	traits					2058:2063	the other curd firming traits	2035:2063	the other curd firming traits	2035:2063	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	1	41	theme	curd	301:304	arg1	parameters					319:328	curd firming (CF) parameters	301:328	curd firming (CF) parameters	301:328	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	9	42	dep	2.65	1512:1515	arg1	to					1509:1510	to	1509:1510	to	1509:1510	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	5	43	theme	genetic	788:794	arg1	MCP					878:880	MCP	878:880	MCP	878:880	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	5	43	theme	genetic	788:794	arg1	parameters					889:898	CF parameters	886:898	CF parameters	886:898	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	5	43	theme	genetic	788:794	arg1	herd					797:800	Additive genetic, herd, and residual correlations	779:827	herd	797:800	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	2	44	theme	Individual	331:340	arg1	samples					347:353	Individual milk samples	331:353	Individual milk samples	331:353	Individual milk samples were collected from 1,114 Sarda ewes reared in 23 farms.					
29428761	0	45	from	firming	135:141	arg1	sheep					152:156	dairy sheep	146:156	dairy sheep	146:156	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	11	46	theme	lactose	1805:1811	arg1	content					1813:1819	lactose content	1805:1819	lactose content	1805:1819	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	0	47	theme	Phenotypic	0:9	arg1	relationships					23:35	Phenotypic and genetic relationships	0:35	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.	0:157	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	2	48	theme	1,114	375:379	arg1	ewes					387:390	1,114 Sarda ewes	375:390	1,114 Sarda ewes reared in 23 farms	375:409	Individual milk samples were collected from 1,114 Sarda ewes reared in 23 farms.					
29428761	8	49	theme	unfavorable	1274:1284	arg1	increase					1286:1293	an unfavorable increase	1271:1293	an unfavorable increase of about 20%	1271:1306	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	4	50	theme	Phenotypic	600:609	arg1	traits					611:616	Phenotypic traits	600:616	Phenotypic traits	600:616	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	0	51	theme	genetic	15:21	arg1	relationships					23:35	Phenotypic and genetic relationships	0:35	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.	0:157	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	1	52	theme	milk	249:252	arg1	composition					254:264	sheep milk composition	243:264	sheep milk composition	243:264	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	14	53	theme	spectra	2377:2383	arg1	prediction					2385:2394	infrared spectra prediction	2368:2394	infrared spectra prediction	2368:2394	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	7	54	theme	milk	1087:1090	arg1	traits					1100:1105	many milk quality traits	1082:1105	many milk quality traits	1082:1105	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	11	55	dep	Lactose	1840:1846	arg1	favorably					1849:1857	favorably	1849:1857	favorably	1849:1857	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	3	56	theme	curd	526:529	arg1	firmness					531:538	curd firmness	526:538	curd firmness	526:538	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	11	57	theme	genetic	1909:1915	arg1	levels					1917:1922	phenotypic and genetic levels	1894:1922	phenotypic and genetic levels	1894:1922	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	1	58	theme	somatic	204:210	arg1	count					217:221	somatic cell count	204:221	somatic cell count	204:221	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	9	59	from	decrease	1528:1535	arg1	min					1485:1487	6.00 to 14.3 min	1472:1487	6.00 to 14.3 min	1472:1487	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	59	from	decrease	1528:1535	arg1	min					1517:1519	1.65 to 2.65 min	1504:1519	1.65 to 2.65 min	1504:1519	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	60	from	increase	1450:1457	arg1	min					1485:1487	6.00 to 14.3 min	1472:1487	6.00 to 14.3 min	1472:1487	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	60	from	increase	1450:1457	arg1	min					1517:1519	1.65 to 2.65 min	1504:1519	1.65 to 2.65 min	1504:1519	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	11	61	theme	phenotypic	1894:1903	arg1	levels					1917:1922	phenotypic and genetic levels	1894:1922	phenotypic and genetic levels	1894:1922	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	1	62	theme	count	217:221	arg1	effect					194:199	the effect	190:199	the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters	190:328	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	7	63	theme	Lactose	1032:1038	arg1	concentration					1040:1052	Lactose concentration	1032:1052	Lactose concentration	1032:1052	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	0	64	from	coagulation	113:123	arg1	sheep					152:156	dairy sheep	146:156	dairy sheep	146:156	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	3	65	theme	somatic	430:436	arg1	count					443:447	somatic cell count	430:447	somatic cell count	430:447	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	1	66	theme	lactose	224:230	arg1	effect					194:199	the effect	190:199	the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters	190:328	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	13	67	theme	milk	2269:2272	arg1	quality					2274:2280	milk quality	2269:2280	milk quality	2269:2280	In conclusion, results reported in the present study suggest that SCS, pH, and lactose affect, contemporarily and independently, milk quality and MCP.					
29428761	4	68	theme	somatic	722:728	arg1	SCS					742:744	SCS	742:744	SCS	742:744	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	4	68	theme	somatic	722:728	arg1	score					735:739	milk somatic cell score	717:739	milk somatic cell score (SCS)	717:745	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	8	69	theme	lactose	1348:1354	arg1	level					1339:1343	the lowest level	1328:1343	the lowest level of lactose	1328:1354	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	14	70	theme	ovine	2481:2485	arg1	milk					2487:2490	ovine milk	2481:2490	ovine milk	2481:2490	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	3	71	theme	single	450:455	arg1	MCP					463:465	single point MCP	450:465	single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60)	450:558	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	9	72	from	min	1485:1487	arg1	RCT					1462:1464	RCT	1462:1464	RCT (from 6.00 to 14.3 min)	1462:1488	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	72	from	min	1485:1487	arg1	decrease					1528:1535	a decrease	1526:1535	a decrease of all the 3 curd firmness traits	1526:1569	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	72	from	min	1485:1487	arg1	increase					1450:1457	an increase	1447:1457	an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min)	1447:1520	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	72	from	min	1485:1487	arg1	k20					1494:1496	k20	1494:1496	k20 (from 1.65 to 2.65 min)	1494:1520	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	73	theme	lower	1385:1389	arg1	values					1378:1383	pH values	1375:1383	pH values lower than 6.56 versus higher than 6.78	1375:1423	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	6	74	theme	phenotypic	949:958	arg1	point					960:964	a phenotypic point	947:964	a phenotypic point of view	947:972	From a phenotypic point of view, higher SCS levels caused a delayed gelification of milk.					
29428761	9	75	theme	curd	1550:1553	arg1	traits					1564:1569	the 3 curd firmness traits	1544:1569	all the 3 curd firmness traits	1540:1569	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	3	76	theme	CF	565:566	arg1	parameters					574:583	CF model parameters	565:583	CF model parameters	565:583	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	6	77	theme	milk	1026:1029	arg1	gelification					1010:1021	a delayed gelification	1000:1021	a delayed gelification of milk	1000:1029	From a phenotypic point of view, higher SCS levels caused a delayed gelification of milk.					
29428761	1	78	theme	present	163:169	arg1	study					171:175	The present study	159:175	The present study	159:175	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	4	79	theme	different	697:705	arg1	levels					707:712	the different levels	693:712	the different levels of milk somatic cell score (SCS), lactose, and pH	693:762	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	9	80	theme	traits	1564:1569	arg1	decrease					1528:1535	a decrease	1526:1535	a decrease of all the 3 curd firmness traits	1526:1569	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	80	theme	traits	1564:1569	arg1	increase					1450:1457	an increase	1447:1457	an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min)	1447:1520	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	7	81	with	significant	1066:1076	arg1	effect					1128:1133	a very intense effect	1113:1133	a very intense effect on both coagulation times and curd firming	1113:1176	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	3	82	theme	rennet	468:473	arg1	time					487:490	rennet coagulation time	468:490	rennet coagulation time	468:490	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	7	83	theme	curd	1165:1168	arg1	firming					1170:1176	curd firming	1165:1176	curd firming	1165:1176	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	10	84	theme	genetic	1579:1585	arg1	point					1587:1591	a genetic point	1577:1591	a genetic point of view	1577:1599	From a genetic point of view, the marginal posterior distribution of heritability estimates evidenced a large and exploitable variability for all 3 phenotypes.					
29428761	0	85	theme	curd	130:133	arg1	firming					135:141	curd firming	130:141	curd firming	130:141	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	3	86	theme	curd	498:501	arg1	time					511:514	curd firming time	498:514	curd firming time	498:514	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	6	87	theme	delayed	1002:1008	arg1	gelification					1010:1021	a delayed gelification	1000:1021	a delayed gelification of milk	1000:1029	From a phenotypic point of view, higher SCS levels caused a delayed gelification of milk.					
29428761	11	88	theme	time	2003:2006	arg1	equation					2008:2015	time equation	2003:2015	time equation	2003:2015	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	10	89	theme	marginal	1606:1613	arg1	distribution					1625:1636	the marginal posterior distribution	1602:1636	the marginal posterior distribution of heritability estimates	1602:1662	From a genetic point of view, the marginal posterior distribution of heritability estimates evidenced a large and exploitable variability for all 3 phenotypes.					
29428761	14	90	theme	potential	2414:2422	arg1	phenotypes					2297:2306	These phenotypes	2291:2306	These phenotypes	2291:2306	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	14	90	theme	potential	2414:2422	arg1	indicators					2424:2433	potential indicators	2414:2433	potential indicators	2414:2433	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	4	91	theme	score	735:739	arg1	levels					707:712	the different levels	693:712	the different levels of milk somatic cell score (SCS), lactose, and pH	693:762	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	8	92	theme	curd	1222:1225	arg1	firming					1227:1233	the curd firming	1218:1233	the curd firming over time equation	1218:1252	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	14	93	theme	available	2316:2324	arg1	phenotypes					2297:2306	These phenotypes	2291:2306	These phenotypes	2291:2306	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	14	93	theme	available	2316:2324	arg1	indicators					2424:2433	potential indicators	2414:2433	potential indicators	2414:2433	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	8	94	dep	traits	1185:1190	arg1	traits					1185:1190	These traits	1179:1190	These traits (RCT, RCT estimated using the curd firming over time equation, and k20)	1179:1262	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	8	94	dep	traits	1185:1190	arg1	RCT					1198:1200	RCT	1198:1200	RCT estimated using the curd firming over time equation	1198:1252	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	8	94	dep	traits	1185:1190	arg1	k20					1259:1261	k20	1259:1261	k20	1259:1261	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	8	94	dep	traits	1185:1190	arg1	RCT					1193:1195	RCT	1193:1195	RCT	1193:1195	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	6	95	theme	SCS	982:984	arg1	levels					986:991	higher SCS levels	975:991	higher SCS levels	975:991	From a phenotypic point of view, higher SCS levels caused a delayed gelification of milk.					
29428761	10	96	theme	heritability	1641:1652	arg1	estimates					1654:1662	heritability estimates	1641:1662	heritability estimates	1641:1662	From a genetic point of view, the marginal posterior distribution of heritability estimates evidenced a large and exploitable variability for all 3 phenotypes.					
29428761	0	97	theme	gland	71:75	arg1	status					84:89	the mammary gland health status	59:89	the mammary gland health status	59:89	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	5	98	theme	milk	860:863	arg1	composition					865:875	milk composition	860:875	milk composition	860:875	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	11	99	theme	firming	2050:2056	arg1	traits					2058:2063	the other curd firming traits	2035:2063	the other curd firming traits	2035:2063	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	0	100	theme	status	84:89	arg1	indicators					45:54	indicators	45:54	indicators of the mammary gland health status	45:89	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	0	100	theme	status	84:89	arg1	coagulation					113:123	coagulation	113:123	coagulation	113:123	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	0	100	theme	status	84:89	arg1	composition					100:110	milk composition	95:110	milk composition	95:110	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	0	100	theme	status	84:89	arg1	firming					135:141	curd firming	130:141	curd firming	130:141	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	3	101	dep	time	487:490	arg1	a45					546:548	a45	546:548	a45	546:548	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	3	101	dep	time	487:490	arg1	a60					555:557	a60	555:557	a60	555:557	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	3	101	dep	time	487:490	arg1	a30					541:543	a30	541:543	a30	541:543	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	5	102	theme	CF	886:887	arg1	parameters					889:898	CF parameters	886:898	CF parameters	886:898	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	5	102	theme	CF	886:887	arg1	herd					797:800	Additive genetic, herd, and residual correlations	779:827	herd	797:800	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	11	103	theme	pH.	1836:1838	arg1	Lactose					1840:1846	pH. Lactose	1836:1846	pH. Lactose (favorably)	1836:1858	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	11	104	theme	other	2039:2043	arg1	traits					2058:2063	the other curd firming traits	2035:2063	the other curd firming traits	2035:2063	The mean intra-farm heritability estimates were 0.173 for SCS, 0.418 for lactose content, and 0.206 for pH. Lactose (favorably), and SCS and pH (unfavorably), at phenotypic and genetic levels, were correlated mainly with RCT and RCT estimated using the curd firming over time equation and scarcely with the other curd firming traits.					
29428761	10	105	theme	large	1676:1680	arg1	variability					1698:1708	a large and exploitable variability	1674:1708	a large and exploitable variability for all 3 phenotypes	1674:1729	From a genetic point of view, the marginal posterior distribution of heritability estimates evidenced a large and exploitable variability for all 3 phenotypes.					
29428761	0	106	theme	milk	95:98	arg1	composition					100:110	milk composition	95:110	milk composition	95:110	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	5	107	theme	Bayesian	923:930	arg1	approach					932:939	a Bayesian approach	921:939	a Bayesian approach	921:939	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	5	108	theme	Additive	779:786	arg1	MCP					878:880	MCP	878:880	MCP	878:880	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	5	108	theme	Additive	779:786	arg1	parameters					889:898	CF parameters	886:898	CF parameters	886:898	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	5	108	theme	Additive	779:786	arg1	herd					797:800	Additive genetic, herd, and residual correlations	779:827	herd	797:800	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	9	109	from	min	1517:1519	arg1	RCT					1462:1464	RCT	1462:1464	RCT (from 6.00 to 14.3 min)	1462:1488	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	109	from	min	1517:1519	arg1	decrease					1528:1535	a decrease	1526:1535	a decrease of all the 3 curd firmness traits	1526:1569	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	109	from	min	1517:1519	arg1	increase					1450:1457	an increase	1447:1457	an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min)	1447:1520	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	109	from	min	1517:1519	arg1	k20					1494:1496	k20	1494:1496	k20 (from 1.65 to 2.65 min)	1494:1520	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	4	110	theme	pH	761:762	arg1	levels					707:712	the different levels	693:712	the different levels of milk somatic cell score (SCS), lactose, and pH	693:762	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
29428761	1	111	theme	firming	306:312	arg1	parameters					319:328	curd firming (CF) parameters	301:328	curd firming (CF) parameters	301:328	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	0	112	from	indicators	45:54	arg1	sheep					152:156	dairy sheep	146:156	dairy sheep	146:156	Phenotypic and genetic relationships between indicators of the mammary gland health status and milk composition, coagulation, and curd firming in dairy sheep.					
29428761	12	113	theme	other	2131:2135	arg1	's					2136:2137	each other's	2126:2137	each other's	2126:2137	The SCS, lactose, and pH were significantly correlated with each other's.					
29428761	1	114	theme	CF	315:316	arg1	parameters					319:328	curd firming (CF) parameters	301:328	curd firming (CF) parameters	301:328	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	7	115	from	effect	1128:1133	arg1	firming					1170:1176	curd firming	1165:1176	curd firming	1165:1176	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	7	115	from	effect	1128:1133	arg1	times					1155:1159	coagulation times	1143:1159	coagulation times	1143:1159	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	7	116	theme	quality	1092:1098	arg1	traits					1100:1105	many milk quality traits	1082:1105	many milk quality traits	1082:1105	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	2	117	theme	Sarda	381:385	arg1	ewes					387:390	1,114 Sarda ewes	375:390	1,114 Sarda ewes reared in 23 farms	375:409	Individual milk samples were collected from 1,114 Sarda ewes reared in 23 farms.					
29428761	1	118	theme	sheep	243:247	arg1	composition					254:264	sheep milk composition	243:264	sheep milk composition	243:264	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	14	119	dep	used	2406:2409	arg1	traits					2435:2440	traits	2435:2440	traits for improving cheese-making ability of ovine milk	2435:2490	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	14	120	theme	recording	2338:2346	arg1	schemes					2348:2354	milk recording schemes	2333:2354	milk recording schemes measured by infrared spectra prediction	2333:2394	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	7	121	theme	many	1082:1085	arg1	traits					1100:1105	many milk quality traits	1082:1105	many milk quality traits	1082:1105	Lactose concentration and pH were significant for many milk quality traits, with a very intense effect on both coagulation times and curd firming.					
29428761	9	122	theme	k20	1494:1496	arg1	decrease					1528:1535	a decrease	1526:1535	a decrease of all the 3 curd firmness traits	1526:1569	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	9	122	theme	k20	1494:1496	arg1	increase					1450:1457	an increase	1447:1457	an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min)	1447:1520	Milk samples with pH values lower than 6.56 versus higher than 6.78 were characterized by an increase of RCT (from 6.00 to 14.3 min) and k20 (from 1.65 to 2.65 min) and a decrease of all the 3 curd firmness traits.					
29428761	14	123	used	used	2406:2409	arg2	indicators					2424:2433	potential indicators	2414:2433	potential indicators	2414:2433	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	14	123	used	used	2406:2409	arg2	phenotypes					2297:2306	These phenotypes	2291:2306	These phenotypes	2291:2306	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	8	124	theme	%	1306:1306	arg1	increase					1286:1293	an unfavorable increase	1271:1293	an unfavorable increase of about 20%	1271:1306	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	2	125	theme	milk	342:345	arg1	samples					347:353	Individual milk samples	331:353	Individual milk samples	331:353	Individual milk samples were collected from 1,114 Sarda ewes reared in 23 farms.					
29428761	1	126	theme	coagulation	267:277	arg1	MCP					291:293	MCP	291:293	MCP	291:293	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	1	126	theme	coagulation	267:277	arg1	properties					279:288	coagulation properties	267:288	coagulation properties (MCP)	267:294	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	14	127	theme	milk	2333:2336	arg1	schemes					2348:2354	milk recording schemes	2333:2354	milk recording schemes measured by infrared spectra prediction	2333:2394	These phenotypes, easily available during milk recording schemes measured by infrared spectra prediction, could be used as potential indicators traits for improving cheese-making ability of ovine milk.					
29428761	3	128	theme	Milk	412:415	arg1	composition					417:427	Milk composition	412:427	Milk composition	412:427	Milk composition, somatic cell count, single point MCP (rennet coagulation time, RCT; curd firming time, k20; and curd firmness, a30, a45, and a60), and CF model parameters were achieved.					
29428761	1	129	theme	cell	212:215	arg1	count					217:221	somatic cell count	204:221	somatic cell count	204:221	The present study investigated the effect of somatic cell count, lactose, and pH on sheep milk composition, coagulation properties (MCP), and curd firming (CF) parameters.					
29428761	5	130	theme	residual	807:814	arg1	correlations					816:827	Additive genetic, herd, and residual correlations	779:827	correlations	816:827	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	10	131	theme	exploitable	1686:1696	arg1	variability					1698:1708	a large and exploitable variability	1674:1708	a large and exploitable variability for all 3 phenotypes	1674:1729	From a genetic point of view, the marginal posterior distribution of heritability estimates evidenced a large and exploitable variability for all 3 phenotypes.					
29428761	8	132	theme	lowest	1332:1337	arg1	level					1339:1343	the lowest level	1328:1343	the lowest level of lactose	1328:1354	These traits (RCT, RCT estimated using the curd firming over time equation, and k20) showed an unfavorable increase of about 20% from the highest to the lowest level of lactose.					
29428761	5	133	with	correlations	816:827	arg1	composition					865:875	milk composition	860:875	milk composition	860:875	Additive genetic, herd, and residual correlations among these 3 traits, and with milk composition, MCP and CF parameters, were inferred using a Bayesian approach.					
29428761	4	134	theme	mixed	654:658	arg1	model					660:664	a mixed model	652:664	a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively	652:776	Phenotypic traits were statistically analyzed using a mixed model to estimate the effects of the different levels of milk somatic cell score (SCS), lactose, and pH, respectively.					
31589646	0	0	theme	membrane	85:92	arg1	integrity					94:102	damaging membrane integrity	76:102	damaging membrane integrity	76:102	Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity.					
31589646	1	1	dep	Li	319:320	arg1	al					325:326	Li et al	319:326	Li et al	319:326	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	8	2	theme	order	997:1001	arg1	level					979:983	a high level	972:983	a high level of membrane order in lipid vesicles	972:1019	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	7	3	from	37°C	868:871	arg1	at					865:866	at 37°C	865:871	at 37°C	865:871	In some cases, cell damage was also prevented by carrying out exchange at close to room temperature (rather than at 37°C).					
31589646	10	4	theme	structure	1412:1420	arg1	aspects					1392:1398	several aspects	1384:1398	several aspects of membrane structure and function	1384:1433	Thus, it should be possible to use lipid exchange to investigate the effect of plasma membrane lipid composition upon several aspects of membrane structure and function.					
31589646	1	5	theme	cultured	271:278	arg1	cells					290:294	cultured mammalian cells	271:294	cultured mammalian cells	271:294	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	0	6	theme	damaging	76:83	arg1	integrity					94:102	damaging membrane integrity	76:102	damaging membrane integrity	76:102	Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity.					
31589646	5	7	theme	exchange	591:598	arg1	step					600:603	the exchange step	587:603	the exchange step	587:603	We found that this cell damage can be reversed/prevented if cells are allowed to recover from the exchange step by incubation in complete growth medium.					
31589646	8	8	with	Exchange	875:882	arg1	lipids					889:894	lipids	889:894	lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles	889:1019	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	1	9	theme	mammalian	280:288	arg1	cells					290:294	cultured mammalian cells	271:294	cultured mammalian cells	271:294	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	8	10	dep	not	926:928	arg1	phosphatidylcholine					943:961	unsaturated phosphatidylcholine	931:961	unsaturated phosphatidylcholine	931:961	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	9	11	theme	plasma	1182:1187	arg1	properties					1198:1207	plasma membrane properties	1182:1207	plasma membrane properties	1182:1207	Importantly, changes in lipid composition and plasma membrane properties after exchange and recovery persisted for several hours.					
31589646	1	12	theme	cells	290:294	arg1	sphingolipids					254:266	sphingolipids	254:266	sphingolipids of cultured mammalian cells	254:294	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	1	12	theme	cells	290:294	arg1	phospholipids					236:248	plasma membrane outer leaflet phospholipids	206:248	plasma membrane outer leaflet phospholipids	206:248	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	8	13	contain	had	1021:1023	arg2	effect					1039:1044	the analogous effect	1025:1044	the analogous effect	1025:1044	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	8	13	contain	had	1021:1023	arg1	Exchange					875:882	Exchange	875:882	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles	875:1019	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	9	14	theme	membrane	1189:1196	arg1	properties					1198:1207	plasma membrane properties	1182:1207	plasma membrane properties	1182:1207	Importantly, changes in lipid composition and plasma membrane properties after exchange and recovery persisted for several hours.					
31589646	6	15	theme	untreated	735:743	arg1	cells					745:749	untreated cells	735:749	untreated cells	735:749	After exchange and transfer to complete growth medium cell growth was similar to that of untreated cells.					
31589646	8	16	theme	plasma	1049:1054	arg1	order					1065:1069	plasma membrane order	1049:1069	plasma membrane order	1049:1069	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	8	17	theme	unsaturated	931:941	arg1	phosphatidylcholine					943:961	unsaturated phosphatidylcholine	931:961	unsaturated phosphatidylcholine	931:961	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	7	18	theme	at	865:866	arg1	37°C					868:871	at 37°C	865:871	at 37°C	865:871	In some cases, cell damage was also prevented by carrying out exchange at close to room temperature (rather than at 37°C).					
31589646	8	19	theme	plasma	1119:1124	arg1	membrane					1126:1133	the plasma membrane	1115:1133	the plasma membrane	1115:1133	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	10	20	theme	plasma	1345:1350	arg1	composition					1367:1377	plasma membrane lipid composition	1345:1377	plasma membrane lipid composition	1345:1377	Thus, it should be possible to use lipid exchange to investigate the effect of plasma membrane lipid composition upon several aspects of membrane structure and function.					
31589646	5	21	theme	cell	512:515	arg1	damage					517:522	this cell damage	507:522	this cell damage	507:522	We found that this cell damage can be reversed/prevented if cells are allowed to recover from the exchange step by incubation in complete growth medium.					
31589646	7	22	theme	cell	767:770	arg1	damage					772:777	cell damage	767:777	cell damage	767:777	In some cases, cell damage was also prevented by carrying out exchange at close to room temperature (rather than at 37°C).					
31589646	1	23	theme	entire	185:190	arg1	population					192:201	virtually the entire population	171:201	virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells	171:294	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	0	24	theme	plasma	10:15	arg1	lipids					40:45	plasma membrane outer leaflet lipids	10:45	plasma membrane outer leaflet lipids	10:45	Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity.					
31589646	1	25	dep	replace	163:169	arg1	Proc					336:339	Proc	336:339	Proc	336:339	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	1	25	dep	replace	163:169	arg1	Li					319:320	Li	319:320	Li	319:320	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	8	26	theme	membrane	1056:1063	arg1	order					1065:1069	plasma membrane order	1049:1069	plasma membrane order	1049:1069	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	1	27	theme	exogenous	301:309	arg1	lipids					311:316	exogenous lipids	301:316	exogenous lipids	301:316	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	10	28	theme	membrane	1352:1359	arg1	composition					1367:1377	plasma membrane lipid composition	1345:1377	plasma membrane lipid composition	1345:1377	Thus, it should be possible to use lipid exchange to investigate the effect of plasma membrane lipid composition upon several aspects of membrane structure and function.					
31589646	0	29	theme	outer	26:30	arg1	lipids					40:45	plasma membrane outer leaflet lipids	10:45	plasma membrane outer leaflet lipids	10:45	Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity.					
31589646	10	30	theme	several	1384:1390	arg1	aspects					1392:1398	several aspects	1384:1398	several aspects of membrane structure and function	1384:1433	Thus, it should be possible to use lipid exchange to investigate the effect of plasma membrane lipid composition upon several aspects of membrane structure and function.					
31589646	1	31	theme	plasma	206:211	arg1	phospholipids					236:248	plasma membrane outer leaflet phospholipids	206:248	plasma membrane outer leaflet phospholipids	206:248	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	0	32	theme	membrane	17:24	arg1	lipids					40:45	plasma membrane outer leaflet lipids	10:45	plasma membrane outer leaflet lipids	10:45	Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity.					
31589646	8	33	dep	do	901:902	arg1	sphingomyelin					905:917	sphingomyelin	905:917	sphingomyelin	905:917	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	1	34	theme	membrane	213:220	arg1	phospholipids					236:248	plasma membrane outer leaflet phospholipids	206:248	plasma membrane outer leaflet phospholipids	206:248	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	8	35	theme	lipid	1093:1097	arg1	localization					1099:1110	exogenous lipid localization	1083:1110	exogenous lipid localization	1083:1110	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	10	36	theme	composition	1367:1377	arg1	effect					1335:1340	the effect	1331:1340	the effect of plasma membrane lipid composition upon several aspects of membrane structure and function	1331:1433	Thus, it should be possible to use lipid exchange to investigate the effect of plasma membrane lipid composition upon several aspects of membrane structure and function.					
31589646	1	37	theme	outer	222:226	arg1	phospholipids					236:248	plasma membrane outer leaflet phospholipids	206:248	plasma membrane outer leaflet phospholipids	206:248	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	0	38	theme	leaflet	32:38	arg1	lipids					40:45	plasma membrane outer leaflet lipids	10:45	plasma membrane outer leaflet lipids	10:45	Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity.					
31589646	4	39	theme	membrane	473:480	arg1	leakiness					482:490	membrane leakiness	473:490	membrane leakiness	473:490	Here, we show if the lipid-to- MαCD ratio is too high or low, cells can round up and develop membrane leakiness.					
31589646	8	40	theme	high	974:977	arg1	level					979:983	a high level	972:983	a high level of membrane order in lipid vesicles	972:1019	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	5	41	from	incubation	608:617	arg1	medium					638:643	complete growth medium	622:643	complete growth medium	622:643	We found that this cell damage can be reversed/prevented if cells are allowed to recover from the exchange step by incubation in complete growth medium.					
31589646	1	42	theme	leaflet	228:234	arg1	phospholipids					236:248	plasma membrane outer leaflet phospholipids	206:248	plasma membrane outer leaflet phospholipids	206:248	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	0	43	theme	exogenous	52:60	arg1	lipid					62:66	exogenous lipid	52:66	exogenous lipid without damaging membrane integrity	52:102	Replacing plasma membrane outer leaflet lipids with exogenous lipid without damaging membrane integrity.					
31589646	9	44	theme	several	1251:1257	arg1	hours					1259:1263	several hours	1251:1263	several hours	1251:1263	Importantly, changes in lipid composition and plasma membrane properties after exchange and recovery persisted for several hours.					
31589646	8	45	theme	membrane	988:995	arg1	order					997:1001	membrane order	988:1001	membrane order	988:1001	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	6	46	theme	cell	700:703	arg1	growth					705:710	cell growth	700:710	cell growth	700:710	After exchange and transfer to complete growth medium cell growth was similar to that of untreated cells.					
31589646	10	47	theme	lipid	1361:1365	arg1	composition					1367:1377	plasma membrane lipid composition	1345:1377	plasma membrane lipid composition	1345:1377	Thus, it should be possible to use lipid exchange to investigate the effect of plasma membrane lipid composition upon several aspects of membrane structure and function.					
31589646	4	48	dep	round	452:456	arg1	up					458:459	up	458:459	up	458:459	Here, we show if the lipid-to- MαCD ratio is too high or low, cells can round up and develop membrane leakiness.					
31589646	10	49	theme	membrane	1403:1410	arg1	structure					1412:1420	membrane structure	1403:1420	membrane structure	1403:1420	Thus, it should be possible to use lipid exchange to investigate the effect of plasma membrane lipid composition upon several aspects of membrane structure and function.					
31589646	10	50	theme	function	1426:1433	arg1	aspects					1392:1398	several aspects	1384:1398	several aspects of membrane structure and function	1384:1433	Thus, it should be possible to use lipid exchange to investigate the effect of plasma membrane lipid composition upon several aspects of membrane structure and function.					
31589646	5	51	theme	complete	622:629	arg1	medium					638:643	complete growth medium	622:643	complete growth medium	622:643	We found that this cell damage can be reversed/prevented if cells are allowed to recover from the exchange step by incubation in complete growth medium.					
31589646	10	52	theme	lipid	1301:1305	arg1	exchange					1307:1314	lipid exchange	1301:1314	lipid exchange	1301:1314	Thus, it should be possible to use lipid exchange to investigate the effect of plasma membrane lipid composition upon several aspects of membrane structure and function.					
31589646	6	53	theme	growth	686:691	arg1	medium					693:698	complete growth medium	677:698	complete growth medium	677:698	After exchange and transfer to complete growth medium cell growth was similar to that of untreated cells.					
31589646	1	54	theme	MαCD-based	130:139	arg1	method					141:146	a MαCD-based method	128:146	a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc	128:339	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	1	55	theme	phospholipids	236:248	arg1	population					192:201	virtually the entire population	171:201	virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells	171:294	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	5	56	theme	growth	631:636	arg1	medium					638:643	complete growth medium	622:643	complete growth medium	622:643	We found that this cell damage can be reversed/prevented if cells are allowed to recover from the exchange step by incubation in complete growth medium.					
31589646	4	57	theme	MαCD	411:414	arg1	ratio					416:420	the lipid-to- MαCD ratio	397:420	the lipid-to- MαCD ratio	397:420	Here, we show if the lipid-to- MαCD ratio is too high or low, cells can round up and develop membrane leakiness.					
31589646	4	57	theme	MαCD	411:414	arg1	high					429:432	high	429:432	high	429:432	Here, we show if the lipid-to- MαCD ratio is too high or low, cells can round up and develop membrane leakiness.					
31589646	6	58	theme	complete	677:684	arg1	medium					693:698	complete growth medium	677:698	complete growth medium	677:698	After exchange and transfer to complete growth medium cell growth was similar to that of untreated cells.					
31589646	7	59	dep	37°C	868:871	arg1	rather					853:858	rather	853:858	rather	853:858	In some cases, cell damage was also prevented by carrying out exchange at close to room temperature (rather than at 37°C).					
31589646	8	60	theme	exogenous	1083:1091	arg1	localization					1099:1110	exogenous lipid localization	1083:1110	exogenous lipid localization	1083:1110	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	9	61	from	changes	1149:1155	arg1	properties					1198:1207	plasma membrane properties	1182:1207	plasma membrane properties	1182:1207	Importantly, changes in lipid composition and plasma membrane properties after exchange and recovery persisted for several hours.					
31589646	9	61	from	changes	1149:1155	arg1	composition					1166:1176	lipid composition	1160:1176	lipid composition	1160:1176	Importantly, changes in lipid composition and plasma membrane properties after exchange and recovery persisted for several hours.					
31589646	4	62	theme	lipid-to-	401:409	arg1	ratio					416:420	the lipid-to- MαCD ratio	397:420	the lipid-to- MαCD ratio	397:420	Here, we show if the lipid-to- MαCD ratio is too high or low, cells can round up and develop membrane leakiness.					
31589646	4	62	theme	lipid-to-	401:409	arg1	high					429:432	high	429:432	high	429:432	Here, we show if the lipid-to- MαCD ratio is too high or low, cells can round up and develop membrane leakiness.					
31589646	1	63	theme	sphingolipids	254:266	arg1	population					192:201	virtually the entire population	171:201	virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells	171:294	We recently introduced a MαCD-based method to efficiently replace virtually the entire population of plasma membrane outer leaflet phospholipids and sphingolipids of cultured mammalian cells with exogenous lipids (Li et al, (2016) Proc.					
31589646	8	64	theme	lipid	1006:1010	arg1	vesicles					1012:1019	lipid vesicles	1006:1019	lipid vesicles	1006:1019	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	9	65	theme	lipid	1160:1164	arg1	composition					1166:1176	lipid composition	1160:1176	lipid composition	1160:1176	Importantly, changes in lipid composition and plasma membrane properties after exchange and recovery persisted for several hours.					
31589646	8	66	from	level	979:983	arg1	vesicles					1012:1019	lipid vesicles	1006:1019	lipid vesicles	1006:1019	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31589646	7	67	dep	room	835:838	arg1	to					832:833	to	832:833	to	832:833	In some cases, cell damage was also prevented by carrying out exchange at close to room temperature (rather than at 37°C).					
31589646	8	68	theme	analogous	1029:1037	arg1	effect					1039:1044	the analogous effect	1025:1044	the analogous effect	1025:1044	Exchange with lipids that do (sphingomyelin) or do not (unsaturated phosphatidylcholine) support a high level of membrane order in lipid vesicles had the analogous effect on plasma membrane order, confirming exogenous lipid localization in the plasma membrane.					
31868609	11	0	theme	low	1480:1482	arg1	limit					1494:1498	low detection limit	1480:1498	low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles	1480:1584	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	8	1	theme	graphene	1110:1117	arg1	film					1119:1122	Electroreduced graphene film	1095:1122	Electroreduced graphene film as substrate	1095:1135	Electroreduced graphene film as substrate provided phenoxy-derivatized dextran (DexP) with abundant binding sites and improved conductivity.					
31868609	13	2	theme	detection	1794:1802	arg1	capabilities					1804:1815	high detection capabilities	1789:1815	high detection capabilities to ConA	1789:1823	The creational pattern of construction strategy achieves high detection capabilities to ConA and expands the applicability of luminol in ECL system.					
31868609	11	3	theme	excellent	1524:1532	arg1	stability					1534:1542	excellent stability	1524:1542	excellent stability	1524:1542	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	3	4	theme	Cu2O	521:524	arg1	AuNPs-Cys-luminol					526:542	Cu2O@AuNPs-Cys-luminol	521:542	Cu2O@AuNPs-Cys-luminol	521:542	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	3	4	theme	Cu2O	521:524	arg1	emitter					512:518	build the composite emitter	492:518	build the composite emitter (Cu2O@AuNPs-Cys-luminol)	492:543	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	1	5	theme	luminol	205:211	arg1	ECL					197:199	ECL	197:199	ECL	197:199	Herein, a credible construction strategy to improve electrochemiluminescence (ECL) of luminol was developed based on Cu2O-Au heterostructures.					
31868609	1	5	theme	luminol	205:211	arg1	electrochemiluminescence					171:194	electrochemiluminescence	171:194	electrochemiluminescence (ECL) of luminol	171:211	Herein, a credible construction strategy to improve electrochemiluminescence (ECL) of luminol was developed based on Cu2O-Au heterostructures.					
31868609	8	6	theme	binding	1195:1201	arg1	sites					1203:1207	abundant binding sites	1186:1207	abundant binding sites	1186:1207	Electroreduced graphene film as substrate provided phenoxy-derivatized dextran (DexP) with abundant binding sites and improved conductivity.					
31868609	10	7	theme	@	1353:1353	arg1	indicator					1412:1420	ECL signal indicator	1401:1420	ECL signal indicator	1401:1420	Then, Cu2O@AuNPs-Cys-luminol was modified on electrode as ECL signal indicator.					
31868609	10	7	theme	@	1353:1353	arg1	AuNPs-Cys-luminol					1354:1370	Cu2O@AuNPs-Cys-luminol	1349:1370	Cu2O@AuNPs-Cys-luminol	1349:1370	Then, Cu2O@AuNPs-Cys-luminol was modified on electrode as ECL signal indicator.					
31868609	2	8	theme	nanocube	333:340	arg1	surface					317:323	surface	317:323	surface of Cu2O nanocube (Cu2O@AuNPs)	317:353	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	13	9	theme	construction	1758:1769	arg1	strategy					1771:1778	construction strategy	1758:1778	construction strategy	1758:1778	The creational pattern of construction strategy achieves high detection capabilities to ConA and expands the applicability of luminol in ECL system.					
31868609	4	10	theme	@	615:615	arg1	AuNPs					616:620	(I) Cu2O@AuNPs	607:620	(I) Cu2O@AuNPs	607:620	The enhancement mechanism was realized by following aspects: (I) Cu2O@AuNPs worked as electrocatalyst for glucose to generate coreactant of H2O2 in situ, avoiding the instability of direct addition of H2O2.					
31868609	5	11	theme	luminol	758:764	arg1	molecules					766:774	(II) luminol molecules	753:774	(II) luminol molecules	753:774	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	11	12	theme	ECL	1427:1429	arg1	immunosensor					1431:1442	The ECL immunosensor	1423:1442	The ECL immunosensor	1423:1442	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	5	13	theme	centralized	822:832	arg1	luminescence					845:856	centralized and strong luminescence	822:856	centralized and strong luminescence at low consumption	822:875	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	12	14	theme	considerable	1666:1677	arg1	progress					1679:1686	considerable progress	1666:1686	considerable progress	1666:1686	Experimental results demonstrated that the proposed construction strategy made considerable progress in ECL efficiency and stability of luminol.					
31868609	4	15	dep	AuNPs	616:620	arg1	I					608:608	I	608:608	I	608:608	The enhancement mechanism was realized by following aspects: (I) Cu2O@AuNPs worked as electrocatalyst for glucose to generate coreactant of H2O2 in situ, avoiding the instability of direct addition of H2O2.					
31868609	5	16	theme	strong	838:843	arg1	luminescence					845:856	centralized and strong luminescence	822:856	centralized and strong luminescence at low consumption	822:875	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	12	17	theme	proposed	1630:1637	arg1	strategy					1652:1659	the proposed construction strategy	1626:1659	the proposed construction strategy	1626:1659	Experimental results demonstrated that the proposed construction strategy made considerable progress in ECL efficiency and stability of luminol.					
31868609	6	18	theme	intramolecular	900:913	arg1	coreactant					915:924	an intramolecular coreactant	897:924	an intramolecular coreactant	897:924	(III) Cys acted as an intramolecular coreactant and directly linked to luminol to increase luminous efficiency.					
31868609	6	18	theme	intramolecular	900:913	arg1	Cys					884:886	Cys	884:886	Cys	884:886	(III) Cys acted as an intramolecular coreactant and directly linked to luminol to increase luminous efficiency.					
31868609	4	19	theme	addition	735:742	arg1	instability					713:723	the instability	709:723	the instability of direct addition of H2O2	709:750	The enhancement mechanism was realized by following aspects: (I) Cu2O@AuNPs worked as electrocatalyst for glucose to generate coreactant of H2O2 in situ, avoiding the instability of direct addition of H2O2.					
31868609	14	20	with	immunoassay	1940:1950	arg1	universality					1957:1968	universality	1957:1968	universality	1957:1968	It is expected to have more potential application value in immunoassay with universality.					
31868609	10	21	theme	signal	1405:1410	arg1	AuNPs-Cys-luminol					1354:1370	Cu2O@AuNPs-Cys-luminol	1349:1370	Cu2O@AuNPs-Cys-luminol	1349:1370	Then, Cu2O@AuNPs-Cys-luminol was modified on electrode as ECL signal indicator.					
31868609	10	21	theme	signal	1405:1410	arg1	indicator					1412:1420	ECL signal indicator	1401:1420	ECL signal indicator	1401:1420	Then, Cu2O@AuNPs-Cys-luminol was modified on electrode as ECL signal indicator.					
31868609	3	22	theme	Cu2O	435:438	arg1	AuNPs					440:444	Cu2O@AuNPs	435:444	Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol)	435:543	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	0	23	theme	Enhanced	0:7	arg1	electrochemiluminescence					9:32	Enhanced electrochemiluminescence	0:32	Enhanced electrochemiluminescence of luminol based on Cu2O-Au heterostructure	0:76	Enhanced electrochemiluminescence of luminol based on Cu2O-Au heterostructure enabled multiple-amplification strategy.					
31868609	1	24	theme	credible	129:136	arg1	strategy					151:158	a credible construction strategy	127:158	a credible construction strategy to improve electrochemiluminescence (ECL) of luminol	127:211	Herein, a credible construction strategy to improve electrochemiluminescence (ECL) of luminol was developed based on Cu2O-Au heterostructures.					
31868609	14	25	theme	potential	1909:1917	arg1	value					1931:1935	more potential application value	1904:1935	more potential application value	1904:1935	It is expected to have more potential application value in immunoassay with universality.					
31868609	0	26	theme	Cu2O-Au	54:60	arg1	heterostructure					62:76	Cu2O-Au heterostructure	54:76	Cu2O-Au heterostructure	54:76	Enhanced electrochemiluminescence of luminol based on Cu2O-Au heterostructure enabled multiple-amplification strategy.					
31868609	9	27	theme	specific	1303:1310	arg1	interaction					1330:1340	the specific carbohydrate-ConA interaction	1299:1340	the specific carbohydrate-ConA interaction	1299:1340	To improve the specificity, DexP was used to identify ConA via the specific carbohydrate-ConA interaction.					
31868609	4	28	theme	H2O2	747:750	arg1	addition					735:742	direct addition	728:742	direct addition of H2O2	728:750	The enhancement mechanism was realized by following aspects: (I) Cu2O@AuNPs worked as electrocatalyst for glucose to generate coreactant of H2O2 in situ, avoiding the instability of direct addition of H2O2.					
31868609	11	29	theme	continuous	1547:1556	arg1	potential					1558:1566	continuous potential	1547:1566	continuous potential scan for 8 cycles	1547:1584	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	2	30	theme	@	347:347	arg1	AuNPs					348:352	Cu2O@AuNPs	343:352	Cu2O@AuNPs	343:352	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	2	30	theme	@	347:347	arg1	nanocube					333:340	Cu2O nanocube	328:340	Cu2O nanocube (Cu2O@AuNPs)	328:353	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	4	31	theme	following	588:596	arg1	aspects					598:604	following aspects	588:604	following aspects	588:604	The enhancement mechanism was realized by following aspects: (I) Cu2O@AuNPs worked as electrocatalyst for glucose to generate coreactant of H2O2 in situ, avoiding the instability of direct addition of H2O2.					
31868609	3	32	theme	composite	502:510	arg1	AuNPs-Cys-luminol					526:542	Cu2O@AuNPs-Cys-luminol	521:542	Cu2O@AuNPs-Cys-luminol	521:542	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	3	32	theme	composite	502:510	arg1	emitter					512:518	build the composite emitter	492:518	build the composite emitter (Cu2O@AuNPs-Cys-luminol)	492:543	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	14	33	theme	more	1904:1907	arg1	value					1931:1935	more potential application value	1904:1935	more potential application value	1904:1935	It is expected to have more potential application value in immunoassay with universality.					
31868609	14	34	theme	application	1919:1929	arg1	value					1931:1935	more potential application value	1904:1935	more potential application value	1904:1935	It is expected to have more potential application value in immunoassay with universality.					
31868609	2	35	theme	spontaneous	358:368	arg1	reaction					380:387	spontaneous reduction reaction	358:387	spontaneous reduction reaction	358:387	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	5	36	dep	molecules	766:774	arg1	II					754:755	II	754:755	II	754:755	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	13	37	theme	strategy	1771:1778	arg1	pattern					1747:1753	The creational pattern	1732:1753	The creational pattern of construction strategy	1732:1778	The creational pattern of construction strategy achieves high detection capabilities to ConA and expands the applicability of luminol in ECL system.					
31868609	3	38	theme	@	525:525	arg1	AuNPs-Cys-luminol					526:542	Cu2O@AuNPs-Cys-luminol	521:542	Cu2O@AuNPs-Cys-luminol	521:542	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	3	38	theme	@	525:525	arg1	emitter					512:518	build the composite emitter	492:518	build the composite emitter (Cu2O@AuNPs-Cys-luminol)	492:543	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	13	39	theme	high	1789:1792	arg1	capabilities					1804:1815	high detection capabilities	1789:1815	high detection capabilities to ConA	1789:1823	The creational pattern of construction strategy achieves high detection capabilities to ConA and expands the applicability of luminol in ECL system.					
31868609	7	40	theme	sandwiched	1023:1032	arg1	immunoassay					1034:1044	a sandwiched immunoassay	1021:1044	a sandwiched immunoassay	1021:1044	To validate the effectiveness, a sandwiched immunoassay was built using concanavalinA (ConA) as analyte.					
31868609	11	41	theme	detection	1484:1492	arg1	limit					1494:1498	low detection limit	1480:1498	low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles	1480:1584	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	5	42	theme	Cu2O	800:803	arg1	AuNPs					805:809	Cu2O@AuNPs	800:809	Cu2O@AuNPs	800:809	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	8	43	theme	Electroreduced	1095:1108	arg1	film					1119:1122	Electroreduced graphene film	1095:1122	Electroreduced graphene film as substrate	1095:1135	Electroreduced graphene film as substrate provided phenoxy-derivatized dextran (DexP) with abundant binding sites and improved conductivity.					
31868609	12	44	theme	luminol	1723:1729	arg1	efficiency					1695:1704	ECL efficiency	1691:1704	ECL efficiency	1691:1704	Experimental results demonstrated that the proposed construction strategy made considerable progress in ECL efficiency and stability of luminol.					
31868609	12	44	theme	luminol	1723:1729	arg1	stability					1710:1718	stability	1710:1718	stability	1710:1718	Experimental results demonstrated that the proposed construction strategy made considerable progress in ECL efficiency and stability of luminol.					
31868609	11	45	dep	potential	1558:1566	arg1	scan					1568:1571	scan	1568:1571	scan for 8 cycles	1568:1584	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	13	46	theme	creational	1736:1745	arg1	pattern					1747:1753	The creational pattern	1732:1753	The creational pattern of construction strategy	1732:1778	The creational pattern of construction strategy achieves high detection capabilities to ConA and expands the applicability of luminol in ECL system.					
31868609	11	47	theme	stability	1534:1542	arg1	limit					1494:1498	low detection limit	1480:1498	low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles	1480:1584	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	8	48	theme	abundant	1186:1193	arg1	sites					1203:1207	abundant binding sites	1186:1207	abundant binding sites	1186:1207	Electroreduced graphene film as substrate provided phenoxy-derivatized dextran (DexP) with abundant binding sites and improved conductivity.					
31868609	11	49	theme	2.9 × 10-5 ng/mL	1503:1518	arg1	limit					1494:1498	low detection limit	1480:1498	low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles	1480:1584	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	10	50	theme	Cu2O	1349:1352	arg1	indicator					1412:1420	ECL signal indicator	1401:1420	ECL signal indicator	1401:1420	Then, Cu2O@AuNPs-Cys-luminol was modified on electrode as ECL signal indicator.					
31868609	10	50	theme	Cu2O	1349:1352	arg1	AuNPs-Cys-luminol					1354:1370	Cu2O@AuNPs-Cys-luminol	1349:1370	Cu2O@AuNPs-Cys-luminol	1349:1370	Then, Cu2O@AuNPs-Cys-luminol was modified on electrode as ECL signal indicator.					
31868609	2	51	theme	Cu2O	328:331	arg1	AuNPs					348:352	Cu2O@AuNPs	343:352	Cu2O@AuNPs	343:352	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	2	51	theme	Cu2O	328:331	arg1	nanocube					333:340	Cu2O nanocube	328:340	Cu2O nanocube (Cu2O@AuNPs)	328:353	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	2	52	theme	gold	273:276	arg1	AuNPs					293:297	AuNPs	293:297	AuNPs	293:297	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	2	52	theme	gold	273:276	arg1	nanoparticles					278:290	gold nanoparticles	273:290	gold nanoparticles (AuNPs)	273:298	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	1	53	theme	Cu2O-Au	236:242	arg1	heterostructures					244:259	Cu2O-Au heterostructures	236:259	Cu2O-Au heterostructures	236:259	Herein, a credible construction strategy to improve electrochemiluminescence (ECL) of luminol was developed based on Cu2O-Au heterostructures.					
31868609	8	54	theme	phenoxy-derivatized	1146:1164	arg1	dextran					1166:1172	phenoxy-derivatized dextran	1146:1172	phenoxy-derivatized dextran (DexP)	1146:1179	Electroreduced graphene film as substrate provided phenoxy-derivatized dextran (DexP) with abundant binding sites and improved conductivity.					
31868609	8	54	theme	phenoxy-derivatized	1146:1164	arg1	DexP					1175:1178	DexP	1175:1178	DexP	1175:1178	Electroreduced graphene film as substrate provided phenoxy-derivatized dextran (DexP) with abundant binding sites and improved conductivity.					
31868609	4	55	theme	Cu2O	611:614	arg1	AuNPs					616:620	(I) Cu2O@AuNPs	607:620	(I) Cu2O@AuNPs	607:620	The enhancement mechanism was realized by following aspects: (I) Cu2O@AuNPs worked as electrocatalyst for glucose to generate coreactant of H2O2 in situ, avoiding the instability of direct addition of H2O2.					
31868609	1	56	theme	construction	138:149	arg1	strategy					151:158	a credible construction strategy	127:158	a credible construction strategy to improve electrochemiluminescence (ECL) of luminol	127:211	Herein, a credible construction strategy to improve electrochemiluminescence (ECL) of luminol was developed based on Cu2O-Au heterostructures.					
31868609	9	57	used	used	1273:1276	arg2	DexP					1264:1267	DexP	1264:1267	DexP	1264:1267	To improve the specificity, DexP was used to identify ConA via the specific carbohydrate-ConA interaction.					
31868609	6	58	dep	acted	888:892	arg1	III					879:881	III	879:881	III	879:881	(III) Cys acted as an intramolecular coreactant and directly linked to luminol to increase luminous efficiency.					
31868609	5	59	theme	low	861:863	arg1	consumption					865:875	low consumption	861:875	low consumption	861:875	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	6	60	attach	linked	939:944	arg1	luminol					949:955	luminol	949:955	luminol	949:955	(III) Cys acted as an intramolecular coreactant and directly linked to luminol to increase luminous efficiency.					
31868609	6	60	attach	linked	939:944	arg2	Cys					884:886	Cys	884:886	Cys	884:886	(III) Cys acted as an intramolecular coreactant and directly linked to luminol to increase luminous efficiency.					
31868609	6	60	attach	linked	939:944	arg2	coreactant					915:924	an intramolecular coreactant	897:924	an intramolecular coreactant	897:924	(III) Cys acted as an intramolecular coreactant and directly linked to luminol to increase luminous efficiency.					
31868609	4	61	theme	H2O2	686:689	arg1	coreactant					672:681	coreactant	672:681	coreactant of H2O2	672:689	The enhancement mechanism was realized by following aspects: (I) Cu2O@AuNPs worked as electrocatalyst for glucose to generate coreactant of H2O2 in situ, avoiding the instability of direct addition of H2O2.					
31868609	14	62	contain	have	1899:1902	arg1	It					1881:1882	It	1881:1882	It	1881:1882	It is expected to have more potential application value in immunoassay with universality.					
31868609	14	62	contain	have	1899:1902	arg2	value					1931:1935	more potential application value	1904:1935	more potential application value	1904:1935	It is expected to have more potential application value in immunoassay with universality.					
31868609	0	63	theme	multiple-amplification	86:107	arg1	strategy					109:116	multiple-amplification strategy	86:116	multiple-amplification strategy	86:116	Enhanced electrochemiluminescence of luminol based on Cu2O-Au heterostructure enabled multiple-amplification strategy.					
31868609	11	64	with	determination	1453:1465	arg1	limit					1494:1498	low detection limit	1480:1498	low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles	1480:1584	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	11	65	theme	ConA	1470:1473	arg1	determination					1453:1465	determination	1453:1465	determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles	1453:1584	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	13	66	theme	luminol	1858:1864	arg1	applicability					1841:1853	the applicability	1837:1853	the applicability of luminol in ECL system	1837:1878	The creational pattern of construction strategy achieves high detection capabilities to ConA and expands the applicability of luminol in ECL system.					
31868609	2	67	theme	Cu2O	343:346	arg1	AuNPs					348:352	Cu2O@AuNPs	343:352	Cu2O@AuNPs	343:352	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	2	67	theme	Cu2O	343:346	arg1	nanocube					333:340	Cu2O nanocube	328:340	Cu2O nanocube (Cu2O@AuNPs)	328:353	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	4	68	theme	direct	728:733	arg1	addition					735:742	direct addition	728:742	direct addition of H2O2	728:750	The enhancement mechanism was realized by following aspects: (I) Cu2O@AuNPs worked as electrocatalyst for glucose to generate coreactant of H2O2 in situ, avoiding the instability of direct addition of H2O2.					
31868609	13	69	theme	ECL	1869:1871	arg1	system					1873:1878	ECL system	1869:1878	ECL system	1869:1878	The creational pattern of construction strategy achieves high detection capabilities to ConA and expands the applicability of luminol in ECL system.					
31868609	5	70	from	consumption	865:875	arg1	luminescence					845:856	centralized and strong luminescence	822:856	centralized and strong luminescence at low consumption	822:875	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	12	71	theme	construction	1639:1650	arg1	strategy					1652:1659	the proposed construction strategy	1626:1659	the proposed construction strategy	1626:1659	Experimental results demonstrated that the proposed construction strategy made considerable progress in ECL efficiency and stability of luminol.					
31868609	3	72	theme	luminol	396:402	arg1	molecules					404:412	luminol molecules	396:412	luminol molecules	396:412	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	10	73	theme	ECL	1401:1403	arg1	AuNPs-Cys-luminol					1354:1370	Cu2O@AuNPs-Cys-luminol	1349:1370	Cu2O@AuNPs-Cys-luminol	1349:1370	Then, Cu2O@AuNPs-Cys-luminol was modified on electrode as ECL signal indicator.					
31868609	10	73	theme	ECL	1401:1403	arg1	indicator					1412:1420	ECL signal indicator	1401:1420	ECL signal indicator	1401:1420	Then, Cu2O@AuNPs-Cys-luminol was modified on electrode as ECL signal indicator.					
31868609	3	74	theme	@	439:439	arg1	AuNPs					440:444	Cu2O@AuNPs	435:444	Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol)	435:543	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	0	75	theme	luminol	37:43	arg1	electrochemiluminescence					9:32	Enhanced electrochemiluminescence	0:32	Enhanced electrochemiluminescence of luminol based on Cu2O-Au heterostructure	0:76	Enhanced electrochemiluminescence of luminol based on Cu2O-Au heterostructure enabled multiple-amplification strategy.					
31868609	12	76	theme	Experimental	1587:1598	arg1	results					1600:1606	Experimental results	1587:1606	Experimental results	1587:1606	Experimental results demonstrated that the proposed construction strategy made considerable progress in ECL efficiency and stability of luminol.					
31868609	4	77	theme	enhancement	550:560	arg1	mechanism					562:570	The enhancement mechanism	546:570	The enhancement mechanism	546:570	The enhancement mechanism was realized by following aspects: (I) Cu2O@AuNPs worked as electrocatalyst for glucose to generate coreactant of H2O2 in situ, avoiding the instability of direct addition of H2O2.					
31868609	5	78	theme	@	804:804	arg1	AuNPs					805:809	Cu2O@AuNPs	800:809	Cu2O@AuNPs	800:809	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	13	79	from	applicability	1841:1853	arg1	system					1873:1878	ECL system	1869:1878	ECL system	1869:1878	The creational pattern of construction strategy achieves high detection capabilities to ConA and expands the applicability of luminol in ECL system.					
31868609	6	80	theme	luminous	969:976	arg1	efficiency					978:987	luminous efficiency	969:987	luminous efficiency	969:987	(III) Cys acted as an intramolecular coreactant and directly linked to luminol to increase luminous efficiency.					
31868609	11	81	theme	potential	1558:1566	arg1	2.9 × 10-5 ng/mL					1503:1518	2.9 × 10-5 ng/mL	1503:1518	2.9 × 10-5 ng/mL	1503:1518	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	11	81	theme	potential	1558:1566	arg1	stability					1534:1542	excellent stability	1524:1542	excellent stability	1524:1542	The ECL immunosensor achieved determination of ConA with low detection limit of 2.9 × 10-5 ng/mL and excellent stability of continuous potential scan for 8 cycles.					
31868609	9	82	theme	carbohydrate-ConA	1312:1328	arg1	interaction					1330:1340	the specific carbohydrate-ConA interaction	1299:1340	the specific carbohydrate-ConA interaction	1299:1340	To improve the specificity, DexP was used to identify ConA via the specific carbohydrate-ConA interaction.					
31868609	1	83	dep	strategy	151:158	arg1	improve					163:169	improve	163:169	to improve electrochemiluminescence (ECL) of luminol	160:211	Herein, a credible construction strategy to improve electrochemiluminescence (ECL) of luminol was developed based on Cu2O-Au heterostructures.					
31868609	3	84	theme	covalent	472:479	arg1	linkage					481:487	covalent linkage	472:487	covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol)	472:543	Then, luminol molecules were concentrated on Cu2O@AuNPs using L-Cysteine (Cys) as covalent linkage to build the composite emitter (Cu2O@AuNPs-Cys-luminol).					
31868609	5	85	attach	attached	788:795	arg2	molecules					766:774	(II) luminol molecules	753:774	(II) luminol molecules	753:774	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	5	85	attach	attached	788:795	arg3	AuNPs					805:809	Cu2O@AuNPs	800:809	Cu2O@AuNPs	800:809	(II) luminol molecules were firmly attached on Cu2O@AuNPs to achieve centralized and strong luminescence at low consumption.					
31868609	2	86	theme	reduction	370:378	arg1	reaction					380:387	spontaneous reduction reaction	358:387	spontaneous reduction reaction	358:387	Summarily, gold nanoparticles (AuNPs) were anchored on surface of Cu2O nanocube (Cu2O@AuNPs) by spontaneous reduction reaction.					
31868609	12	87	theme	ECL	1691:1693	arg1	efficiency					1695:1704	ECL efficiency	1691:1704	ECL efficiency	1691:1704	Experimental results demonstrated that the proposed construction strategy made considerable progress in ECL efficiency and stability of luminol.					
30196464	0	0	theme	metals	84:89	arg1	removal					53:59	simultaneous removal	40:59	simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water	40:137	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	7	1	from	Application	1117:1127	arg1	PW					1172:1173	real oilfield PW	1158:1173	real oilfield PW	1158:1173	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	6	2	theme	film	1063:1066	arg1	diffusion					1068:1076	film diffusion	1063:1076	film diffusion	1063:1076	Kinetic models' evaluation demonstrated that the adsorption kinetics of metals on CTS-A-MMT are initially controlled by the chemical reaction (film diffusion) followed by intra-particle diffusion.					
30196464	6	2	theme	film	1063:1066	arg1	reaction					1053:1060	the chemical reaction	1040:1060	the chemical reaction (film diffusion) followed by intra-particle diffusion	1040:1114	Kinetic models' evaluation demonstrated that the adsorption kinetics of metals on CTS-A-MMT are initially controlled by the chemical reaction (film diffusion) followed by intra-particle diffusion.					
30196464	5	3	from	CTS-A-MMT	803:811	arg1	model					828:832	a monolayer model	816:832	a monolayer model (best fitted by Langmuir model)	816:864	The adsorption data indicated that crude oil and all studied metals (except As) were adsorbed to CTS-A-MMT in a monolayer model (best fitted by Langmuir model), while As adsorption fits well with Freundlich model.					
30196464	6	4	theme	adsorption	969:978	arg1	kinetics					980:987	the adsorption kinetics	965:987	the adsorption kinetics of metals on CTS-A-MMT	965:1010	Kinetic models' evaluation demonstrated that the adsorption kinetics of metals on CTS-A-MMT are initially controlled by the chemical reaction (film diffusion) followed by intra-particle diffusion.					
30196464	0	5	theme	heavy	78:82	arg1	metals					84:89	heavy metals	78:89	heavy metals	78:89	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	6	6	from	kinetics	980:987	arg1	CTS-A-MMT					1002:1010	CTS-A-MMT	1002:1010	CTS-A-MMT	1002:1010	Kinetic models' evaluation demonstrated that the adsorption kinetics of metals on CTS-A-MMT are initially controlled by the chemical reaction (film diffusion) followed by intra-particle diffusion.					
30196464	2	7	theme	acid-activated	330:343	arg1	montmorillonite					345:359	acid-activated montmorillonite	330:359	acid-activated montmorillonite	330:359	In this study, a novel low-cost bio-adsorbent was prepared from shrimp shell and acid-activated montmorillonite.					
30196464	0	8	theme	synthetic	96:104	arg1	water					133:137	synthetic and real oilfield-produced water	96:137	synthetic and real oilfield-produced water	96:137	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	2	9	theme	novel	266:270	arg1	bio-adsorbent					281:293	a novel low-cost bio-adsorbent	264:293	a novel low-cost bio-adsorbent	264:293	In this study, a novel low-cost bio-adsorbent was prepared from shrimp shell and acid-activated montmorillonite.					
30196464	7	10	from	PW	1172:1173	arg1	Application					1117:1127	Application	1117:1127	Application of the prepared CTS-A-MMT in real oilfield PW	1117:1173	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	5	11	theme	crude	741:745	arg1	oil					747:749	crude oil	741:749	crude oil	741:749	The adsorption data indicated that crude oil and all studied metals (except As) were adsorbed to CTS-A-MMT in a monolayer model (best fitted by Langmuir model), while As adsorption fits well with Freundlich model.					
30196464	5	12	theme	adsorption	710:719	arg1	data					721:724	The adsorption data	706:724	The adsorption data	706:724	The adsorption data indicated that crude oil and all studied metals (except As) were adsorbed to CTS-A-MMT in a monolayer model (best fitted by Langmuir model), while As adsorption fits well with Freundlich model.					
30196464	7	13	theme	%	1215:1215	arg1	efficiency					1193:1202	removal efficiency	1185:1202	removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments	1185:1284	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	0	14	theme	real	110:113	arg1	water					133:137	synthetic and real oilfield-produced water	96:137	synthetic and real oilfield-produced water	96:137	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	7	15	theme	%	1234:1234	arg1	efficiency					1193:1202	removal efficiency	1185:1202	removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments	1185:1284	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	5	16	theme	monolayer	818:826	arg1	model					828:832	a monolayer model	816:832	a monolayer model (best fitted by Langmuir model)	816:864	The adsorption data indicated that crude oil and all studied metals (except As) were adsorbed to CTS-A-MMT in a monolayer model (best fitted by Langmuir model), while As adsorption fits well with Freundlich model.					
30196464	4	17	theme	metal	641:645	arg1	species					647:653	five cationic and anionic metal species	615:653	five cationic and anionic metal species	615:653	The synthesized CTS-A-MMT was applied to remove simultaneously five cationic and anionic metal species and crude oil from synthetic and real oilfield PW.					
30196464	8	18	theme	ocean	1478:1482	arg1	criteria					1494:1501	the ocean discharge criteria	1474:1501	the ocean discharge criteria	1474:1501	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	7	19	theme	simultaneous	1253:1264	arg1	experiments					1274:1284	simultaneous removal experiments	1253:1284	simultaneous removal experiments	1253:1284	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	7	20	theme	real	1158:1161	arg1	PW					1172:1173	real oilfield PW	1158:1173	real oilfield PW	1158:1173	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	4	21	theme	cationic	620:627	arg1	species					647:653	five cationic and anionic metal species	615:653	five cationic and anionic metal species	615:653	The synthesized CTS-A-MMT was applied to remove simultaneously five cationic and anionic metal species and crude oil from synthetic and real oilfield PW.					
30196464	3	22	theme	analysis	427:434	arg1	results					366:372	The results	362:372	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique	362:457	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	0	23	theme	waste-sourced	9:21	arg1	biocomposite					23:34	waste-sourced biocomposite	9:34	waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water	9:137	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	6	24	theme	Kinetic	920:926	arg1	models					928:933	Kinetic models'	920:934	Kinetic models' evaluation	920:945	Kinetic models' evaluation demonstrated that the adsorption kinetics of metals on CTS-A-MMT are initially controlled by the chemical reaction (film diffusion) followed by intra-particle diffusion.					
30196464	9	25	theme	crude	1638:1642	arg1	oil					1644:1646	crude oil	1638:1646	crude oil	1638:1646	It is concluded that the prepared CTS-A-MMT composite is a low-cost and effective adsorbent for treating wastewater contaminated with crude oil and heavy metals (i.e., PW).					
30196464	7	26	dep	93	1213:1214	arg1	to					1210:1211	to	1210:1211	to	1210:1211	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	7	27	from	CTS-A-MMT	1145:1153	arg1	PW					1172:1173	real oilfield PW	1158:1173	real oilfield PW	1158:1173	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	7	28	theme	removal	1266:1272	arg1	experiments					1274:1284	simultaneous removal experiments	1253:1284	simultaneous removal experiments	1253:1284	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	3	29	theme	spectroscopy	383:394	arg1	results					366:372	The results	362:372	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique	362:457	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	4	30	theme	synthesized	556:566	arg1	CTS-A-MMT					568:576	The synthesized CTS-A-MMT	552:576	The synthesized CTS-A-MMT	552:576	The synthesized CTS-A-MMT was applied to remove simultaneously five cationic and anionic metal species and crude oil from synthetic and real oilfield PW.					
30196464	5	31	theme	Freundlich	902:911	arg1	model					913:917	Freundlich model	902:917	Freundlich model	902:917	The adsorption data indicated that crude oil and all studied metals (except As) were adsorbed to CTS-A-MMT in a monolayer model (best fitted by Langmuir model), while As adsorption fits well with Freundlich model.					
30196464	3	32	theme	EDX	422:424	arg1	analysis					427:434	energy dispersive X-ray (EDX) analysis	397:434	energy dispersive X-ray (EDX) analysis	397:434	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	7	33	theme	prepared	1136:1143	arg1	CTS-A-MMT					1145:1153	the prepared CTS-A-MMT	1132:1153	the prepared CTS-A-MMT in real oilfield PW	1132:1173	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	8	34	theme	discharge	1484:1492	arg1	criteria					1494:1501	the ocean discharge criteria	1474:1501	the ocean discharge criteria	1474:1501	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	9	35	theme	prepared	1529:1536	arg1	composite					1548:1556	the prepared CTS-A-MMT composite	1525:1556	the prepared CTS-A-MMT composite	1525:1556	It is concluded that the prepared CTS-A-MMT composite is a low-cost and effective adsorbent for treating wastewater contaminated with crude oil and heavy metals (i.e., PW).					
30196464	9	35	theme	prepared	1529:1536	arg1	low-cost					1563:1570	low-cost	1563:1570	low-cost	1563:1570	It is concluded that the prepared CTS-A-MMT composite is a low-cost and effective adsorbent for treating wastewater contaminated with crude oil and heavy metals (i.e., PW).					
30196464	1	36	theme	various	188:194	arg1	pollutants					196:205	various pollutants	188:205	various pollutants	188:205	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	7	37	theme	CTS-A-MMT	1145:1153	arg1	Application					1117:1127	Application	1117:1127	Application of the prepared CTS-A-MMT in real oilfield PW	1117:1173	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	0	38	theme	oilfield-produced	115:131	arg1	water					133:137	synthetic and real oilfield-produced water	96:137	synthetic and real oilfield-produced water	96:137	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	1	39	contain	contains	179:186	arg2	pollutants					196:205	various pollutants	188:205	various pollutants	188:205	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	1	39	contain	contains	179:186	arg1	Oil-					140:143	Oil-	140:143	Oil-	140:143	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	1	39	contain	contains	179:186	arg1	water					162:166	gas-produced water	149:166	gas-produced water	149:166	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	1	39	contain	contains	179:186	arg1	PW					169:170	PW	169:170	PW	169:170	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	1	39	contain	contains	179:186	arg1	threat					222:227	an enormous threat	210:227	an enormous threat to the environment	210:246	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	2	40	theme	low-cost	272:279	arg1	bio-adsorbent					281:293	a novel low-cost bio-adsorbent	264:293	a novel low-cost bio-adsorbent	264:293	In this study, a novel low-cost bio-adsorbent was prepared from shrimp shell and acid-activated montmorillonite.					
30196464	3	41	theme	technique	449:457	arg1	results					366:372	The results	362:372	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique	362:457	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	3	42	theme	dispersive	404:413	arg1	analysis					427:434	energy dispersive X-ray (EDX) analysis	397:434	energy dispersive X-ray (EDX) analysis	397:434	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	6	43	theme	intra-particle	1091:1104	arg1	diffusion					1106:1114	intra-particle diffusion	1091:1114	intra-particle diffusion	1091:1114	Kinetic models' evaluation demonstrated that the adsorption kinetics of metals on CTS-A-MMT are initially controlled by the chemical reaction (film diffusion) followed by intra-particle diffusion.					
30196464	7	44	theme	crude	1240:1244	arg1	oil					1246:1248	crude oil	1240:1248	crude oil	1240:1248	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	8	45	theme	studied	1346:1352	arg1	metals					1354:1359	studied metals	1346:1359	studied metals	1346:1359	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	4	46	theme	synthetic	674:682	arg1	PW					702:703	synthetic and real oilfield PW	674:703	PW	702:703	The synthesized CTS-A-MMT was applied to remove simultaneously five cationic and anionic metal species and crude oil from synthetic and real oilfield PW.					
30196464	4	47	theme	crude	659:663	arg1	oil					665:667	crude oil	659:667	crude oil	659:667	The synthesized CTS-A-MMT was applied to remove simultaneously five cationic and anionic metal species and crude oil from synthetic and real oilfield PW.					
30196464	0	48	theme	simultaneous	40:51	arg1	removal					53:59	simultaneous removal	40:59	simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water	40:137	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	3	49	theme	X-ray	415:419	arg1	analysis					427:434	energy dispersive X-ray (EDX) analysis	397:434	energy dispersive X-ray (EDX) analysis	397:434	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	4	50	theme	anionic	633:639	arg1	species					647:653	five cationic and anionic metal species	615:653	five cationic and anionic metal species	615:653	The synthesized CTS-A-MMT was applied to remove simultaneously five cationic and anionic metal species and crude oil from synthetic and real oilfield PW.					
30196464	0	51	theme	crude	64:68	arg1	oil					70:72	crude oil	64:72	crude oil	64:72	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	9	52	dep	PW	1672:1673	arg1	i.e.					1666:1669	i.e.	1666:1669	i.e.	1666:1669	It is concluded that the prepared CTS-A-MMT composite is a low-cost and effective adsorbent for treating wastewater contaminated with crude oil and heavy metals (i.e., PW).					
30196464	8	53	theme	additional	1299:1308	arg1	ions					1310:1313	additional ions	1299:1313	additional ions	1299:1313	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	5	54	theme	studied	759:765	arg1	metals					767:772	all studied metals	755:772	all studied metals (except As)	755:784	The adsorption data indicated that crude oil and all studied metals (except As) were adsorbed to CTS-A-MMT in a monolayer model (best fitted by Langmuir model), while As adsorption fits well with Freundlich model.					
30196464	5	55	theme	As	873:874	arg1	adsorption					876:885	As adsorption	873:885	As adsorption	873:885	The adsorption data indicated that crude oil and all studied metals (except As) were adsorbed to CTS-A-MMT in a monolayer model (best fitted by Langmuir model), while As adsorption fits well with Freundlich model.					
30196464	3	56	theme	energy	397:402	arg1	analysis					427:434	energy dispersive X-ray (EDX) analysis	397:434	energy dispersive X-ray (EDX) analysis	397:434	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	8	57	theme	treated	1450:1456	arg1	PW					1458:1459	treated PW	1450:1459	treated PW	1450:1459	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	7	58	from	efficiency	1193:1202	arg1	experiments					1274:1284	simultaneous removal experiments	1253:1284	simultaneous removal experiments	1253:1284	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	8	59	theme	metals	1354:1359	arg1	removal					1335:1341	the removal	1331:1341	the removal of studied metals and crude oil	1331:1373	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	3	60	theme	chitosan-activated	478:495	arg1	CTS-A-MMT					514:522	CTS-A-MMT	514:522	CTS-A-MMT	514:522	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	3	60	theme	chitosan-activated	478:495	arg1	montmorillonite					497:511	the chitosan-activated montmorillonite	474:511	the chitosan-activated montmorillonite (CTS-A-MMT)	474:523	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	8	61	from	Presence	1287:1294	arg1	PW					1318:1319	PW	1318:1319	PW	1318:1319	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	8	62	theme	oil	1371:1373	arg1	removal					1335:1341	the removal	1331:1341	the removal of studied metals and crude oil	1331:1373	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	4	63	theme	real	688:691	arg1	PW					702:703	synthetic and real oilfield PW	674:703	PW	702:703	The synthesized CTS-A-MMT was applied to remove simultaneously five cationic and anionic metal species and crude oil from synthetic and real oilfield PW.					
30196464	2	64	theme	shrimp	313:318	arg1	shell					320:324	shrimp shell	313:324	shrimp shell	313:324	In this study, a novel low-cost bio-adsorbent was prepared from shrimp shell and acid-activated montmorillonite.					
30196464	5	65	theme	Langmuir	850:857	arg1	model					859:863	Langmuir model	850:863	Langmuir model	850:863	The adsorption data indicated that crude oil and all studied metals (except As) were adsorbed to CTS-A-MMT in a monolayer model (best fitted by Langmuir model), while As adsorption fits well with Freundlich model.					
30196464	1	66	theme	enormous	213:220	arg1	Oil-					140:143	Oil-	140:143	Oil-	140:143	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	1	66	theme	enormous	213:220	arg1	water					162:166	gas-produced water	149:166	gas-produced water	149:166	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	1	66	theme	enormous	213:220	arg1	threat					222:227	an enormous threat	210:227	an enormous threat to the environment	210:246	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	8	67	theme	ions	1310:1313	arg1	Presence					1287:1294	Presence	1287:1294	Presence of additional ions in PW	1287:1319	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	8	68	theme	pollutants	1436:1445	arg1	concentration					1402:1414	the concentration	1398:1414	the concentration of the investigated pollutants in treated PW	1398:1459	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	8	68	theme	pollutants	1436:1445	arg1	less					1464:1467	less	1464:1467	less	1464:1467	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	8	69	theme	crude	1365:1369	arg1	oil					1371:1373	crude oil	1365:1373	crude oil	1365:1373	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	1	70	theme	gas-produced	149:160	arg1	Oil-					140:143	Oil-	140:143	Oil-	140:143	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	1	70	theme	gas-produced	149:160	arg1	water					162:166	gas-produced water	149:166	gas-produced water	149:166	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	1	70	theme	gas-produced	149:160	arg1	threat					222:227	an enormous threat	210:227	an enormous threat to the environment	210:246	Oil- and gas-produced water (PW) which contains various pollutants is an enormous threat to the environment.					
30196464	6	71	theme	metals	992:997	arg1	kinetics					980:987	the adsorption kinetics	965:987	the adsorption kinetics of metals on CTS-A-MMT	965:1010	Kinetic models' evaluation demonstrated that the adsorption kinetics of metals on CTS-A-MMT are initially controlled by the chemical reaction (film diffusion) followed by intra-particle diffusion.					
30196464	8	72	from	concentration	1402:1414	arg1	PW					1458:1459	treated PW	1450:1459	treated PW	1450:1459	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	8	73	theme	investigated	1423:1434	arg1	pollutants					1436:1445	the investigated pollutants	1419:1445	the investigated pollutants	1419:1445	Presence of additional ions in PW decreased the removal of studied metals and crude oil considerably; however, the concentration of the investigated pollutants in treated PW is less than the ocean discharge criteria.					
30196464	9	74	theme	CTS-A-MMT	1538:1546	arg1	composite					1548:1556	the prepared CTS-A-MMT composite	1525:1556	the prepared CTS-A-MMT composite	1525:1556	It is concluded that the prepared CTS-A-MMT composite is a low-cost and effective adsorbent for treating wastewater contaminated with crude oil and heavy metals (i.e., PW).					
30196464	9	74	theme	CTS-A-MMT	1538:1546	arg1	low-cost					1563:1570	low-cost	1563:1570	low-cost	1563:1570	It is concluded that the prepared CTS-A-MMT composite is a low-cost and effective adsorbent for treating wastewater contaminated with crude oil and heavy metals (i.e., PW).					
30196464	3	75	theme	SEM-EDX	441:447	arg1	technique					449:457	SEM-EDX technique	441:457	SEM-EDX technique	441:457	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	4	76	theme	oilfield	693:700	arg1	PW					702:703	synthetic and real oilfield PW	674:703	PW	702:703	The synthesized CTS-A-MMT was applied to remove simultaneously five cationic and anionic metal species and crude oil from synthetic and real oilfield PW.					
30196464	6	77	theme	chemical	1044:1051	arg1	diffusion					1068:1076	film diffusion	1063:1076	film diffusion	1063:1076	Kinetic models' evaluation demonstrated that the adsorption kinetics of metals on CTS-A-MMT are initially controlled by the chemical reaction (film diffusion) followed by intra-particle diffusion.					
30196464	6	77	theme	chemical	1044:1051	arg1	reaction					1053:1060	the chemical reaction	1040:1060	the chemical reaction (film diffusion) followed by intra-particle diffusion	1040:1114	Kinetic models' evaluation demonstrated that the adsorption kinetics of metals on CTS-A-MMT are initially controlled by the chemical reaction (film diffusion) followed by intra-particle diffusion.					
30196464	0	78	from	water	133:137	arg1	removal					53:59	simultaneous removal	40:59	simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water	40:137	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	0	79	theme	oil	70:72	arg1	removal					53:59	simultaneous removal	40:59	simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water	40:137	Improved waste-sourced biocomposite for simultaneous removal of crude oil and heavy metals from synthetic and real oilfield-produced water.					
30196464	7	80	theme	oilfield	1163:1170	arg1	PW					1172:1173	real oilfield PW	1158:1173	real oilfield PW	1158:1173	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30196464	3	81	theme	FT-IR	377:381	arg1	spectroscopy					383:394	FT-IR spectroscopy	377:394	FT-IR spectroscopy	377:394	The results of FT-IR spectroscopy, energy dispersive X-ray (EDX) analysis, and SEM-EDX technique indicated that the chitosan-activated montmorillonite (CTS-A-MMT) was prepared successfully.					
30196464	9	82	theme	heavy	1652:1656	arg1	metals					1658:1663	heavy metals	1652:1663	heavy metals (i.e., PW)	1652:1674	It is concluded that the prepared CTS-A-MMT composite is a low-cost and effective adsorbent for treating wastewater contaminated with crude oil and heavy metals (i.e., PW).					
30196464	9	82	theme	heavy	1652:1656	arg1	PW					1672:1673	PW	1672:1673	PW	1672:1673	It is concluded that the prepared CTS-A-MMT composite is a low-cost and effective adsorbent for treating wastewater contaminated with crude oil and heavy metals (i.e., PW).					
30196464	7	83	theme	removal	1185:1191	arg1	efficiency					1193:1202	removal efficiency	1185:1202	removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments	1185:1284	Application of the prepared CTS-A-MMT in real oilfield PW indicated removal efficiency of 65 to 93% for metals and 87% for crude oil in simultaneous removal experiments.					
30734555	0	0	theme	Mesenchymal	78:88	arg1	Engineering					118:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	8	1	theme	bone	1617:1620	arg1	regeneration					1629:1640	osteoprogenitor-cell-based bone tissue regeneration	1590:1640	osteoprogenitor-cell-based bone tissue regeneration	1590:1640	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	6	2	from	expression	1170:1179	arg1	MSCs					1228:1231	MSCs	1228:1231	MSCs	1228:1231	Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro.					
30734555	7	3	theme	BMP9-induced	1314:1325	arg1	formation					1332:1340	BMP9-induced bone formation	1314:1340	BMP9-induced bone formation	1314:1340	The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis.					
30734555	1	4	theme	medical	249:255	arg1	conditions					257:266	certain medical conditions	241:266	certain medical conditions	241:266	Effective bone tissue engineering can restore bone and skeletal functions that are impaired by traumas and/or certain medical conditions.					
30734555	2	5	theme	biomechanical	357:369	arg1	forces					371:376	biomechanical forces	357:376	biomechanical forces	357:376	Bone is a complex tissue and functions through orchestrated interactions between cells, biomechanical forces, and biofactors.					
30734555	0	6	theme	Cell	95:98	arg1	Engineering					118:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	8	7	theme	bioprintable	1519:1530	arg1	hydrogel					1532:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	8	7	theme	bioprintable	1519:1530	arg1	scaffold					1577:1584	a novel scaffold	1569:1584	a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration	1569:1640	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	4	8	theme	controlled-assembly	890:908	arg1	fashion					910:916	a pH-triggered controlled-assembly fashion	875:916	a pH-triggered controlled-assembly fashion	875:916	We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion.					
30734555	3	9	theme	composite	541:549	arg1	hydrogel					564:571	a composite nanoparticle hydrogel	539:571	a composite nanoparticle hydrogel	539:571	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	6	10	theme	markers	1217:1223	arg1	expression					1170:1179	the expression	1166:1179	the expression of osteoblastic regulators and bone markers in MSCs	1166:1231	Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro.					
30734555	3	11	theme	ideal	407:411	arg1	materials					422:430	ideal scaffold materials	407:430	ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration	407:503	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	0	12	theme	Stem	90:93	arg1	Engineering					118:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	3	13	theme	nanoparticle	551:562	arg1	hydrogel					564:571	a composite nanoparticle hydrogel	539:571	a composite nanoparticle hydrogel	539:571	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	7	14	theme	bone	1327:1330	arg1	formation					1332:1340	BMP9-induced bone formation	1314:1340	BMP9-induced bone formation	1314:1340	The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis.					
30734555	2	15	theme	complex	279:285	arg1	tissue					287:292	a complex tissue	277:292	a complex tissue	277:292	Bone is a complex tissue and functions through orchestrated interactions between cells, biomechanical forces, and biofactors.					
30734555	2	15	theme	complex	279:285	arg1	Bone					269:272	Bone	269:272	Bone	269:272	Bone is a complex tissue and functions through orchestrated interactions between cells, biomechanical forces, and biofactors.					
30734555	3	16	theme	tissue	485:490	arg1	regeneration					492:503	effective mesenchymal stem cell (MSC)-based bone tissue regeneration	436:503	effective mesenchymal stem cell (MSC)-based bone tissue regeneration	436:503	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	0	17	theme	Bone	106:109	arg1	Engineering					118:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	4	18	theme	acidic	838:843	arg1	nanoparticles					858:870	the acidic freeze-dried nanoparticles	834:870	the acidic freeze-dried nanoparticles	834:870	We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion.					
30734555	6	19	theme	CMCh-ACP	1111:1118	arg1	hydrogel					1120:1127	the CMCh-ACP hydrogel itself	1107:1134	the CMCh-ACP hydrogel itself	1107:1134	Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro.					
30734555	6	19	theme	CMCh-ACP	1111:1118	arg1	osteoinductive					1139:1152	osteoinductive	1139:1152	osteoinductive	1139:1152	Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro.					
30734555	5	20	theme	cell	1024:1027	arg1	adhesion					1029:1036	cell adhesion	1024:1036	cell adhesion	1024:1036	The CMCh-ACP hydrogel exhibits excellent biocompatibility and effectively supports MSC proliferation and cell adhesion.					
30734555	7	21	theme	long-term	1397:1405	arg1	osteogenesis					1415:1426	long-term ectopic osteogenesis	1397:1426	long-term ectopic osteogenesis	1397:1426	The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis.					
30734555	3	22	theme	stem	458:461	arg1	cell					463:466	effective mesenchymal stem cell	436:466	effective mesenchymal stem cell	436:466	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	0	23	theme	Based	100:104	arg1	Engineering					118:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	5	24	theme	CMCh-ACP	923:930	arg1	hydrogel					932:939	The CMCh-ACP hydrogel	919:939	The CMCh-ACP hydrogel	919:939	The CMCh-ACP hydrogel exhibits excellent biocompatibility and effectively supports MSC proliferation and cell adhesion.					
30734555	6	25	theme	regulators	1197:1206	arg1	expression					1170:1179	the expression	1166:1179	the expression of osteoblastic regulators and bone markers in MSCs	1166:1231	Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro.					
30734555	6	26	theme	osteogenic	1079:1088	arg1	differentiation					1090:1104	BMP9-induced osteogenic differentiation	1066:1104	BMP9-induced osteogenic differentiation	1066:1104	Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro.					
30734555	0	27	theme	pH-Triggered	2:13	arg1	Scaffold					65:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold	0:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.	0:129	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	2	28	theme	orchestrated	316:327	arg1	interactions					329:340	orchestrated interactions	316:340	orchestrated interactions between cells, biomechanical forces, and biofactors	316:392	Bone is a complex tissue and functions through orchestrated interactions between cells, biomechanical forces, and biofactors.					
30734555	3	29	theme	effective	436:444	arg1	cell					463:466	effective mesenchymal stem cell	436:466	effective mesenchymal stem cell	436:466	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	7	30	theme	CMCh-ACP	1247:1254	arg1	scaffold					1256:1263	The CMCh-ACP scaffold	1243:1263	The CMCh-ACP scaffold	1243:1263	The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis.					
30734555	4	31	theme	CMCh-ACP	713:720	arg1	hydrogel					722:729	the CMCh-ACP hydrogel	709:729	the CMCh-ACP hydrogel	709:729	We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion.					
30734555	3	32	theme	carboxymethyl	586:598	arg1	CMCh					610:613	CMCh	610:613	CMCh	610:613	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	3	32	theme	carboxymethyl	586:598	arg1	chitosan					600:607	carboxymethyl chitosan	586:607	carboxymethyl chitosan (CMCh)	586:614	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	1	33	theme	skeletal	186:193	arg1	functions					195:203	skeletal functions	186:203	skeletal functions	186:203	Effective bone tissue engineering can restore bone and skeletal functions that are impaired by traumas and/or certain medical conditions.					
30734555	0	34	theme	Self-Assembled	16:29	arg1	Scaffold					65:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold	0:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.	0:129	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	7	35	dep	efficiency	1287:1296	arg1	the					1283:1285	the	1283:1285	the	1283:1285	The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis.					
30734555	8	36	theme	injectable	1504:1513	arg1	hydrogel					1532:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	8	36	theme	injectable	1504:1513	arg1	scaffold					1577:1584	a novel scaffold	1569:1584	a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration	1569:1640	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	8	37	theme	pH-responsive	1466:1478	arg1	hydrogel					1532:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	8	37	theme	pH-responsive	1466:1478	arg1	scaffold					1577:1584	a novel scaffold	1569:1584	a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration	1569:1640	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	6	38	theme	osteoblastic	1184:1195	arg1	regulators					1197:1206	osteoblastic regulators	1184:1206	osteoblastic regulators	1184:1206	Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro.					
30734555	0	39	theme	Tissue	111:116	arg1	Engineering					118:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	Mesenchymal Stem Cell Based Bone Tissue Engineering	78:128	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	5	40	theme	MSC	1002:1004	arg1	proliferation					1006:1018	MSC proliferation	1002:1018	MSC proliferation	1002:1018	The CMCh-ACP hydrogel exhibits excellent biocompatibility and effectively supports MSC proliferation and cell adhesion.					
30734555	3	41	theme	-based	473:478	arg1	regeneration					492:503	effective mesenchymal stem cell (MSC)-based bone tissue regeneration	436:503	effective mesenchymal stem cell (MSC)-based bone tissue regeneration	436:503	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	7	42	theme	formation	1332:1340	arg1	maturity					1302:1309	maturity	1302:1309	maturity	1302:1309	The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis.					
30734555	7	42	theme	formation	1332:1340	arg1	efficiency					1287:1296	efficiency	1287:1296	efficiency	1287:1296	The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis.					
30734555	8	43	theme	CMCh-ACP	1495:1502	arg1	hydrogel					1532:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	8	43	theme	CMCh-ACP	1495:1502	arg1	scaffold					1577:1584	a novel scaffold	1569:1584	a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration	1569:1640	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	3	44	theme	mesenchymal	446:456	arg1	cell					463:466	effective mesenchymal stem cell	436:466	effective mesenchymal stem cell	436:466	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	7	45	theme	bone	1369:1372	arg1	resorption					1374:1383	bone resorption	1369:1383	bone resorption occurred in long-term ectopic osteogenesis	1369:1426	The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis.					
30734555	4	46	theme	aqueous	800:806	arg1	dispersion					808:817	an aqueous dispersion	797:817	an aqueous dispersion	797:817	We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion.					
30734555	3	47	dep	phosphate	638:646	arg1	designated					655:664	designated	655:664	designated as CMCh-ACP hydrogel	655:685	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	0	48	theme	Hybrid	49:54	arg1	Scaffold					65:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold	0:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.	0:129	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	4	49	theme	freeze-dried	845:856	arg1	nanoparticles					858:870	the acidic freeze-dried nanoparticles	834:870	the acidic freeze-dried nanoparticles	834:870	We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion.					
30734555	6	50	theme	bone	1212:1215	arg1	markers					1217:1223	bone markers	1212:1223	bone markers	1212:1223	Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro.					
30734555	0	51	theme	Bioprintable	36:47	arg1	Scaffold					65:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold	0:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.	0:129	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	7	52	theme	ectopic	1407:1413	arg1	osteogenesis					1415:1426	long-term ectopic osteogenesis	1397:1426	long-term ectopic osteogenesis	1397:1426	The CMCh-ACP scaffold markedly enhances the efficiency and maturity of BMP9-induced bone formation in vivo, while suppressing bone resorption occurred in long-term ectopic osteogenesis.					
30734555	3	53	theme	CMCh-ACP	669:676	arg1	hydrogel					678:685	CMCh-ACP hydrogel	669:685	CMCh-ACP hydrogel	669:685	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	3	54	theme	scaffold	413:420	arg1	materials					422:430	ideal scaffold materials	407:430	ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration	407:503	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	3	55	dep	-based	473:478	arg1	cell					463:466	effective mesenchymal stem cell	436:466	effective mesenchymal stem cell	436:466	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	8	56	theme	novel	1571:1575	arg1	scaffold					1577:1584	a novel scaffold	1569:1584	a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration	1569:1640	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	8	56	theme	novel	1571:1575	arg1	hydrogel					1532:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	3	57	theme	calcium	630:636	arg1	ACP					649:651	ACP	649:651	ACP	649:651	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	3	57	theme	calcium	630:636	arg1	phosphate					638:646	amorphous calcium phosphate	620:646	amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel)	620:686	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	8	58	theme	self-assembled	1480:1493	arg1	hydrogel					1532:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel	1462:1539	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	8	58	theme	self-assembled	1480:1493	arg1	scaffold					1577:1584	a novel scaffold	1569:1584	a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration	1569:1640	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	3	59	theme	bone	480:483	arg1	regeneration					492:503	effective mesenchymal stem cell (MSC)-based bone tissue regeneration	436:503	effective mesenchymal stem cell (MSC)-based bone tissue regeneration	436:503	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	8	60	theme	osteoprogenitor-cell-based	1590:1615	arg1	regeneration					1629:1640	osteoprogenitor-cell-based bone tissue regeneration	1590:1640	osteoprogenitor-cell-based bone tissue regeneration	1590:1640	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	4	61	theme	glucono	768:774	arg1	GDL					787:789	GDL	787:789	GDL	787:789	We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion.					
30734555	4	61	theme	glucono	768:774	arg1	δ-lactone					776:784	glucono δ-lactone	768:784	glucono δ-lactone (GDL)	768:790	We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion.					
30734555	1	62	theme	Effective	131:139	arg1	engineering					153:163	Effective bone tissue engineering	131:163	Effective bone tissue engineering	131:163	Effective bone tissue engineering can restore bone and skeletal functions that are impaired by traumas and/or certain medical conditions.					
30734555	4	63	theme	pH-triggered	877:888	arg1	fashion					910:916	a pH-triggered controlled-assembly fashion	875:916	a pH-triggered controlled-assembly fashion	875:916	We demonstrate that the CMCh-ACP hydrogel is readily prepared by incorporating glucono δ-lactone (GDL) into an aqueous dispersion or rehydrating the acidic freeze-dried nanoparticles in a pH-triggered controlled-assembly fashion.					
30734555	5	64	theme	excellent	950:958	arg1	biocompatibility					960:975	excellent biocompatibility	950:975	excellent biocompatibility	950:975	The CMCh-ACP hydrogel exhibits excellent biocompatibility and effectively supports MSC proliferation and cell adhesion.					
30734555	1	65	theme	bone	141:144	arg1	engineering					153:163	Effective bone tissue engineering	131:163	Effective bone tissue engineering	131:163	Effective bone tissue engineering can restore bone and skeletal functions that are impaired by traumas and/or certain medical conditions.					
30734555	6	66	theme	BMP9-induced	1066:1077	arg1	differentiation					1090:1104	BMP9-induced osteogenic differentiation	1066:1104	BMP9-induced osteogenic differentiation	1066:1104	Moreover, while augmenting BMP9-induced osteogenic differentiation, the CMCh-ACP hydrogel itself is osteoinductive and induces the expression of osteoblastic regulators and bone markers in MSCs in vitro.					
30734555	0	67	theme	Hydrogel	56:63	arg1	Scaffold					65:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold	0:72	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.	0:129	A pH-Triggered, Self-Assembled, and Bioprintable Hybrid Hydrogel Scaffold for Mesenchymal Stem Cell Based Bone Tissue Engineering.					
30734555	8	68	theme	tissue	1622:1627	arg1	regeneration					1629:1640	osteoprogenitor-cell-based bone tissue regeneration	1590:1640	osteoprogenitor-cell-based bone tissue regeneration	1590:1640	Thus, these results suggest that the pH-responsive self-assembled CMCh-ACP injectable and bioprintable hydrogel may be further exploited as a novel scaffold for osteoprogenitor-cell-based bone tissue regeneration.					
30734555	3	69	theme	amorphous	620:628	arg1	ACP					649:651	ACP	649:651	ACP	649:651	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	3	69	theme	amorphous	620:628	arg1	phosphate					638:646	amorphous calcium phosphate	620:646	amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel)	620:686	To identify ideal scaffold materials for effective mesenchymal stem cell (MSC)-based bone tissue regeneration, here we develop and characterize a composite nanoparticle hydrogel by combining carboxymethyl chitosan (CMCh) and amorphous calcium phosphate (ACP) (designated as CMCh-ACP hydrogel).					
30734555	1	70	theme	tissue	146:151	arg1	engineering					153:163	Effective bone tissue engineering	131:163	Effective bone tissue engineering	131:163	Effective bone tissue engineering can restore bone and skeletal functions that are impaired by traumas and/or certain medical conditions.					
30734555	1	71	theme	certain	241:247	arg1	conditions					257:266	certain medical conditions	241:266	certain medical conditions	241:266	Effective bone tissue engineering can restore bone and skeletal functions that are impaired by traumas and/or certain medical conditions.					
31038537	1	0	theme	Cupriavidus	296:306	arg1	necator					308:314	Cupriavidus necator	296:314	Cupriavidus necator	296:314	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
31038537	2	1	theme	composite	678:686	arg1	design					701:706	rotational central composite experimental design	659:706	rotational central composite experimental design	659:706	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	1	2	theme	present	133:139	arg1	work					141:144	The present work	129:144	The present work	129:144	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
31038537	4	3	theme	C.	957:958	arg1	necator					960:966	C. necator	957:966	C. necator	957:966	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	0	4	theme	inexpensive	58:68	arg1	Comparison					0:9	Comparison	0:9	Comparison of acid and enzymatic hydrolysis of pectin	0:52	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	0	4	theme	inexpensive	58:68	arg1	source					70:75	as inexpensive source	55:75	as inexpensive source to cell growth of Cupriavidus necator	55:113	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	1	5	theme	necator	308:314	arg1	growth					286:291	cell growth	281:291	cell growth of Cupriavidus necator	281:314	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
31038537	2	6	theme	inexpensive	557:567	arg1	substrates					569:578	inexpensive substrates	557:578	inexpensive substrates For this	557:587	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	7	dep	acid	609:612	arg1	procedures					639:648	procedures	639:648	procedures	639:648	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	0	8	theme	cell	80:83	arg1	growth					85:90	cell growth	80:90	cell growth of Cupriavidus necator	80:113	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	5	9	theme	enzymatic	1000:1008	arg1	method					1021:1026	the enzymatic hydrolysis method	996:1026	the enzymatic hydrolysis method	996:1026	The results indicated that the enzymatic hydrolysis method was the most efficient, with a 93.0% yield of RCs, while the yield for acid hydrolysis was 60.0%.					
31038537	5	9	theme	enzymatic	1000:1008	arg1	efficient					1041:1049	efficient	1041:1049	efficient	1041:1049	The results indicated that the enzymatic hydrolysis method was the most efficient, with a 93.0% yield of RCs, while the yield for acid hydrolysis was 60.0%.					
31038537	3	10	dep	design	805:810	arg1	both					787:790	both	787:790	both	787:790	It was analyzed as a variable response for both experimental design, the RCs' production.					
31038537	3	10	dep	design	805:810	arg1	production					822:831	the RCs' production	813:831	the RCs' production	813:831	It was analyzed as a variable response for both experimental design, the RCs' production.					
31038537	7	11	theme	other	1452:1456	arg1	substrates					1458:1467	other substrates	1452:1467	other substrates	1452:1467	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	3	12	theme	variable	765:772	arg1	It					744:745	It	744:745	It	744:745	It was analyzed as a variable response for both experimental design, the RCs' production.					
31038537	3	12	theme	variable	765:772	arg1	response					774:781	a variable response	763:781	a variable response for both experimental design, the RCs' production	763:831	It was analyzed as a variable response for both experimental design, the RCs' production.					
31038537	2	13	theme	polyhydroxybutyrate	474:492	arg1	production					460:469	production	460:469	production of polyhydroxybutyrate (PHB)	460:498	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	5	14	theme	hydrolysis	1010:1019	arg1	method					1021:1026	the enzymatic hydrolysis method	996:1026	the enzymatic hydrolysis method	996:1026	The results indicated that the enzymatic hydrolysis method was the most efficient, with a 93.0% yield of RCs, while the yield for acid hydrolysis was 60.0%.					
31038537	5	14	theme	hydrolysis	1010:1019	arg1	efficient					1041:1049	efficient	1041:1049	efficient	1041:1049	The results indicated that the enzymatic hydrolysis method was the most efficient, with a 93.0% yield of RCs, while the yield for acid hydrolysis was 60.0%.					
31038537	6	15	theme	optimum	1131:1137	arg1	concentration					1190:1202	an enzyme concentration	1180:1202	an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin)	1180:1266	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	6	15	theme	optimum	1131:1137	arg1	conditions					1139:1148	The optimum conditions	1127:1148	The optimum conditions for enzymatic hydrolysis	1127:1173	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	7	16	contain	containing	1356:1365	arg1	media					1350:1354	the media	1346:1354	the media containing pectin hydrolysates	1346:1385	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	7	16	contain	containing	1356:1365	arg2	hydrolysates					1374:1385	pectin hydrolysates	1367:1385	pectin hydrolysates	1367:1385	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	4	17	theme	study	885:889	arg1	kinetics					891:898	study kinetics	885:898	study kinetics of RCs' production	885:917	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	2	18	contain	has	336:338	arg1	microorganism					322:334	This microorganism	317:334	This microorganism	317:334	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	18	contain	has	336:338	arg2	importance					346:355	great importance	340:355	great importance industrial	340:366	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	19	theme	pectin	715:720	arg1	concentration					722:734	pectin concentration	715:734	pectin concentration (1.0%)	715:741	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	19	theme	pectin	715:720	arg1	%					740:740	1.0%	737:740	1.0%	737:740	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	5	20	theme	93.0	1059:1062	arg1	%					1063:1063	%	1063:1063	%	1063:1063	The results indicated that the enzymatic hydrolysis method was the most efficient, with a 93.0% yield of RCs, while the yield for acid hydrolysis was 60.0%.					
31038537	4	21	theme	cell	942:945	arg1	growth					947:952	cell growth	942:952	cell growth of C. necator	942:966	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	2	22	theme	studied	434:440	arg1	protein					407:413	potential single cell protein	385:413	potential single cell protein (SCP)	385:419	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	22	theme	studied	434:440	arg1	microorganism					442:454	the most studied microorganism	425:454	the most studied microorganism for production of polyhydroxybutyrate (PHB)	425:498	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	6	23	theme	enzyme	1183:1188	arg1	concentration					1190:1202	an enzyme concentration	1180:1202	an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin)	1180:1266	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	6	23	theme	enzyme	1183:1188	arg1	conditions					1139:1148	The optimum conditions	1127:1148	The optimum conditions for enzymatic hydrolysis	1127:1173	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	2	24	theme	industrial	357:366	arg1	importance					346:355	great importance	340:355	great importance industrial	340:366	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	7	25	theme	satisfactory	1323:1334	arg1	growth					1336:1341	satisfactory growth	1323:1341	satisfactory growth	1323:1341	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	2	26	theme	enzymatic	618:626	arg1	hydrolysis					628:637	enzymatic hydrolysis	618:637	enzymatic hydrolysis	618:637	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	6	27	theme	UI/g	1213:1216	arg1	concentration					1190:1202	an enzyme concentration	1180:1202	an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin)	1180:1266	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	6	27	theme	UI/g	1213:1216	arg1	agitation					1275:1283	an agitation	1272:1283	an agitation speed of 230.3 rpm	1272:1302	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	6	27	theme	UI/g	1213:1216	arg1	conditions					1139:1148	The optimum conditions	1127:1148	The optimum conditions for enzymatic hydrolysis	1127:1173	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	1	28	theme	appropriate	168:178	arg1	methods					180:186	the appropriate methods	164:186	the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator	164:314	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
31038537	0	29	theme	necator	107:113	arg1	growth					85:90	cell growth	80:90	cell growth of Cupriavidus necator	80:113	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	2	30	theme	cell	402:405	arg1	SCP					416:418	SCP	416:418	SCP	416:418	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	30	theme	cell	402:405	arg1	protein					407:413	potential single cell protein	385:413	potential single cell protein (SCP)	385:419	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	30	theme	cell	402:405	arg1	microorganism					442:454	the most studied microorganism	425:454	the most studied microorganism for production of polyhydroxybutyrate (PHB)	425:498	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	0	31	theme	acid	14:17	arg1	Comparison					0:9	Comparison	0:9	Comparison of acid and enzymatic hydrolysis of pectin	0:52	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	0	31	theme	acid	14:17	arg1	source					70:75	as inexpensive source	55:75	as inexpensive source to cell growth of Cupriavidus necator	55:113	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	5	32	theme	RCs	1074:1076	arg1	yield					1065:1069	a 93.0% yield	1057:1069	a 93.0% yield of RCs	1057:1076	The results indicated that the enzymatic hydrolysis method was the most efficient, with a 93.0% yield of RCs, while the yield for acid hydrolysis was 60.0%.					
31038537	0	33	theme	Cupriavidus	95:105	arg1	necator					107:113	Cupriavidus necator	95:113	Cupriavidus necator	95:113	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	7	34	theme	specific	1393:1400	arg1	μMax					1416:1419	μMax	1416:1419	μMax	1416:1419	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	7	34	theme	specific	1393:1400	arg1	rates					1409:1413	specific growth rates	1393:1413	specific growth rates (μMax) similar to those reported for other substrates	1393:1467	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	2	35	theme	single	395:400	arg1	SCP					416:418	SCP	416:418	SCP	416:418	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	35	theme	single	395:400	arg1	protein					407:413	potential single cell protein	385:413	potential single cell protein (SCP)	385:419	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	35	theme	single	395:400	arg1	microorganism					442:454	the most studied microorganism	425:454	the most studied microorganism for production of polyhydroxybutyrate (PHB)	425:498	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	0	36	theme	hydrolysis	33:42	arg1	Comparison					0:9	Comparison	0:9	Comparison of acid and enzymatic hydrolysis of pectin	0:52	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	0	36	theme	hydrolysis	33:42	arg1	source					70:75	as inexpensive source	55:75	as inexpensive source to cell growth of Cupriavidus necator	55:113	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	2	37	theme	potential	385:393	arg1	SCP					416:418	SCP	416:418	SCP	416:418	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	37	theme	potential	385:393	arg1	protein					407:413	potential single cell protein	385:413	potential single cell protein (SCP)	385:419	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	2	37	theme	potential	385:393	arg1	microorganism					442:454	the most studied microorganism	425:454	the most studied microorganism for production of polyhydroxybutyrate (PHB)	425:498	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	1	38	theme	hydrolysis	191:200	arg1	methods					180:186	the appropriate methods	164:186	the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator	164:314	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
31038537	0	39	theme	enzymatic	23:31	arg1	hydrolysis					33:42	enzymatic hydrolysis	23:42	enzymatic hydrolysis of pectin	23:52	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	4	40	theme	procedure	862:870	arg1	conditions					843:852	The best conditions	834:852	The best conditions of each procedure	834:870	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	4	40	theme	procedure	862:870	arg1	substrate					928:936	a substrate	926:936	a substrate for cell growth of C. necator	926:966	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	7	41	theme	C.	1305:1306	arg1	necator					1308:1314	C. necator	1305:1314	C. necator	1305:1314	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	2	42	theme	central	670:676	arg1	design					701:706	rotational central composite experimental design	659:706	rotational central composite experimental design	659:706	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	6	43	theme	International	1219:1231	arg1	Unit					1233:1236	International Unit	1219:1236	International Unit of enzyme per gram of pectin	1219:1265	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	0	44	theme	pectin	47:52	arg1	acid					14:17	acid	14:17	acid	14:17	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	0	44	theme	pectin	47:52	arg1	hydrolysis					33:42	enzymatic hydrolysis	23:42	enzymatic hydrolysis of pectin	23:52	Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator.					
31038537	6	45	theme	enzyme	1241:1246	arg1	Unit					1233:1236	International Unit	1219:1236	International Unit of enzyme per gram of pectin	1219:1265	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	2	46	theme	cell	533:536	arg1	concentration					538:550	high cell concentration	528:550	high cell concentration	528:550	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	1	47	dep	investigated	146:157	arg1	methods					180:186	the appropriate methods	164:186	the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator	164:314	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
31038537	2	48	theme	rotational	659:668	arg1	design					701:706	rotational central composite experimental design	659:706	rotational central composite experimental design	659:706	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	6	49	dep	UI/g	1213:1216	arg1	Unit					1233:1236	International Unit	1219:1236	International Unit of enzyme per gram of pectin	1219:1265	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	1	50	theme	cell	281:284	arg1	growth					286:291	cell growth	281:291	cell growth of Cupriavidus necator	281:314	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
31038537	2	51	theme	high	528:531	arg1	concentration					538:550	high cell concentration	528:550	high cell concentration	528:550	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	7	52	theme	pectin	1367:1372	arg1	hydrolysates					1374:1385	pectin hydrolysates	1367:1385	pectin hydrolysates	1367:1385	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	7	53	theme	growth	1402:1407	arg1	μMax					1416:1419	μMax	1416:1419	μMax	1416:1419	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	7	53	theme	growth	1402:1407	arg1	rates					1409:1413	specific growth rates	1393:1413	specific growth rates (μMax) similar to those reported for other substrates	1393:1467	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	5	54	theme	%	1063:1063	arg1	yield					1065:1069	a 93.0% yield	1057:1069	a 93.0% yield of RCs	1057:1076	The results indicated that the enzymatic hydrolysis method was the most efficient, with a 93.0% yield of RCs, while the yield for acid hydrolysis was 60.0%.					
31038537	6	55	theme	enzymatic	1154:1162	arg1	hydrolysis					1164:1173	enzymatic hydrolysis	1154:1173	enzymatic hydrolysis	1154:1173	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	1	56	theme	pectin	205:210	arg1	hydrolysis					191:200	hydrolysis	191:200	hydrolysis of pectin	191:210	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
31038537	4	57	theme	best	838:841	arg1	conditions					843:852	The best conditions	834:852	The best conditions of each procedure	834:870	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	4	57	theme	best	838:841	arg1	substrate					928:936	a substrate	926:936	a substrate for cell growth of C. necator	926:966	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	4	58	used	used	877:880	arg2	substrate					928:936	a substrate	926:936	a substrate for cell growth of C. necator	926:966	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	4	58	used	used	877:880	arg2	conditions					843:852	The best conditions	834:852	The best conditions of each procedure	834:870	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	6	59	theme	pectin	1260:1265	arg1	gram					1252:1255	gram	1252:1255	gram of pectin	1252:1265	The optimum conditions for enzymatic hydrolysis were an enzyme concentration of 10.01 UI/g (International Unit of enzyme per gram of pectin) and an agitation speed of 230.3 rpm.					
31038537	1	60	dep	methods	180:186	arg1	what					159:162	what	159:162	what	159:162	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
31038537	5	61	with	efficient	1041:1049	arg1	yield					1065:1069	a 93.0% yield	1057:1069	a 93.0% yield of RCs	1057:1076	The results indicated that the enzymatic hydrolysis method was the most efficient, with a 93.0% yield of RCs, while the yield for acid hydrolysis was 60.0%.					
31038537	2	62	theme	great	340:344	arg1	importance					346:355	great importance	340:355	great importance industrial	340:366	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	3	63	theme	experimental	792:803	arg1	design					805:810	experimental design	792:810	experimental design	792:810	It was analyzed as a variable response for both experimental design, the RCs' production.					
31038537	7	64	theme	similar	1422:1428	arg1	μMax					1416:1419	μMax	1416:1419	μMax	1416:1419	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	7	64	theme	similar	1422:1428	arg1	rates					1409:1413	specific growth rates	1393:1413	specific growth rates (μMax) similar to those reported for other substrates	1393:1467	C. necator showed satisfactory growth in the media containing pectin hydrolysates, with specific growth rates (μMax) similar to those reported for other substrates.					
31038537	2	65	theme	experimental	688:699	arg1	design					701:706	rotational central composite experimental design	659:706	rotational central composite experimental design	659:706	This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%).					
31038537	5	66	theme	acid	1099:1102	arg1	hydrolysis					1104:1113	acid hydrolysis	1099:1113	acid hydrolysis	1099:1113	The results indicated that the enzymatic hydrolysis method was the most efficient, with a 93.0% yield of RCs, while the yield for acid hydrolysis was 60.0%.					
31038537	4	67	theme	necator	960:966	arg1	growth					947:952	cell growth	942:952	cell growth of C. necator	942:966	The best conditions of each procedure were used in study kinetics of RCs' production and as a substrate for cell growth of C. necator.					
31038537	1	68	theme	compounds	225:233	arg1	production					241:250	compounds (RCs) production	225:250	compounds (RCs) production	225:250	Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator.					
30172819	2	0	theme	initial	474:480	arg1	concentration					482:494	initial concentration	474:494	initial concentration of Pb(II), adsorption time, pH, and temperature	474:542	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	0	theme	initial	474:480	arg1	dosage					466:471	adsorbent dosage	456:471	adsorbent dosage	456:471	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	1	theme	@	346:346	arg1	composite					351:359	The novel McAPT@Alg composite	331:359	The novel McAPT@Alg composite	331:359	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	4	2	theme	adsorption	899:908	arg1	kinetics					910:917	the adsorption kinetics	895:917	the adsorption kinetics	895:917	The experimental data were suitable for application to the Langmuir isotherm model, and the adsorption kinetics agreed with the pseudo-second-order model.					
30172819	3	3	theme	adsorption	661:670	arg1	equilibrium					672:682	adsorption equilibrium	661:682	adsorption equilibrium	661:682	The obtained results demonstrated that adsorption equilibrium could be reached within 1.5 h, with the maximum adsorption capacity being 471.20 mg g-1 while the temperature was 297.2 K.					
30172819	2	4	theme	adsorption	551:560	arg1	capacity					562:569	the adsorption capacity	547:569	the adsorption capacity	547:569	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	1	5	theme	effective	168:176	arg1	Pb					178:179	an effective Pb	165:179	an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg)	165:277	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	1	6	theme	sodium	257:262	arg1	Alg					274:276	Alg	274:276	Alg	274:276	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	1	6	theme	sodium	257:262	arg1	alginate					264:271	sodium alginate	257:271	sodium alginate (Alg)	257:277	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	4	7	theme	pseudo-second-order	935:953	arg1	model					955:959	the pseudo-second-order model	931:959	the pseudo-second-order model	931:959	The experimental data were suitable for application to the Langmuir isotherm model, and the adsorption kinetics agreed with the pseudo-second-order model.					
30172819	5	8	theme	McAPT	1110:1114	arg1	Alg					1116:1118	McAPT@Alg	1110:1118	McAPT@Alg	1110:1118	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	4	9	theme	isotherm	875:882	arg1	model					884:888	the Langmuir isotherm model	862:888	the Langmuir isotherm model	862:888	The experimental data were suitable for application to the Langmuir isotherm model, and the adsorption kinetics agreed with the pseudo-second-order model.					
30172819	0	10	theme	aqueous	109:115	arg1	solutions					117:125	aqueous solutions	109:125	aqueous solutions	109:125	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.					
30172819	4	11	theme	Langmuir	866:873	arg1	model					884:888	the Langmuir isotherm model	862:888	the Langmuir isotherm model	862:888	The experimental data were suitable for application to the Langmuir isotherm model, and the adsorption kinetics agreed with the pseudo-second-order model.					
30172819	2	12	from	effects	445:451	arg1	capacity					562:569	the adsorption capacity	547:569	the adsorption capacity	547:569	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	13	theme	batch	603:607	arg1	experiments					609:619	batch experiments	603:619	batch experiments	603:619	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	14	theme	Pb	499:500	arg1	concentration					482:494	initial concentration	474:494	initial concentration of Pb(II), adsorption time, pH, and temperature	474:542	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	14	theme	Pb	499:500	arg1	dosage					466:471	adsorbent dosage	456:471	adsorbent dosage	456:471	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	15	theme	dosage	466:471	arg1	effects					445:451	the effects	441:451	the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity	441:569	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	3	16	theme	obtained	626:633	arg1	results					635:641	The obtained results	622:641	The obtained results	622:641	The obtained results demonstrated that adsorption equilibrium could be reached within 1.5 h, with the maximum adsorption capacity being 471.20 mg g-1 while the temperature was 297.2 K.					
30172819	3	17	theme	maximum	724:730	arg1	471.20 mg g-1					758:770	471.20 mg g-1	758:770	471.20 mg g-1	758:770	The obtained results demonstrated that adsorption equilibrium could be reached within 1.5 h, with the maximum adsorption capacity being 471.20 mg g-1 while the temperature was 297.2 K.					
30172819	3	17	theme	maximum	724:730	arg1	capacity					743:750	the maximum adsorption capacity	720:750	the maximum adsorption capacity	720:750	The obtained results demonstrated that adsorption equilibrium could be reached within 1.5 h, with the maximum adsorption capacity being 471.20 mg g-1 while the temperature was 297.2 K.					
30172819	0	18	theme	carboxyl-functionalized	24:46	arg1	beads					77:81	magnetic carboxyl-functionalized attapulgite/calcium alginate beads	15:81	magnetic carboxyl-functionalized attapulgite/calcium alginate beads	15:81	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.					
30172819	3	19	theme	adsorption	732:741	arg1	471.20 mg g-1					758:770	471.20 mg g-1	758:770	471.20 mg g-1	758:770	The obtained results demonstrated that adsorption equilibrium could be reached within 1.5 h, with the maximum adsorption capacity being 471.20 mg g-1 while the temperature was 297.2 K.					
30172819	3	19	theme	adsorption	732:741	arg1	capacity					743:750	the maximum adsorption capacity	720:750	the maximum adsorption capacity	720:750	The obtained results demonstrated that adsorption equilibrium could be reached within 1.5 h, with the maximum adsorption capacity being 471.20 mg g-1 while the temperature was 297.2 K.					
30172819	1	20	from	alginate	264:271	arg1	adsorbent					185:193	adsorbent	185:193	adsorbent	185:193	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	5	21	attach	removing	1124:1131	arg1	water					1158:1162	contaminated water	1145:1162	contaminated water	1145:1162	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	5	21	attach	removing	1124:1131	arg2	potential					1097:1105	the excellent potential	1083:1105	the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water	1083:1162	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	1	22	theme	adsorbent	185:193	arg1	Pb					178:179	an effective Pb	165:179	an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg)	165:277	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	0	23	theme	magnetic	15:22	arg1	beads					77:81	magnetic carboxyl-functionalized attapulgite/calcium alginate beads	15:81	magnetic carboxyl-functionalized attapulgite/calcium alginate beads	15:81	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.					
30172819	2	24	theme	time	518:521	arg1	concentration					482:494	initial concentration	474:494	initial concentration of Pb(II), adsorption time, pH, and temperature	474:542	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	24	theme	time	518:521	arg1	dosage					466:471	adsorbent dosage	456:471	adsorbent dosage	456:471	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	25	theme	McAPT	341:345	arg1	composite					351:359	The novel McAPT@Alg composite	331:359	The novel McAPT@Alg composite	331:359	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	0	26	theme	alginate	68:75	arg1	beads					77:81	magnetic carboxyl-functionalized attapulgite/calcium alginate beads	15:81	magnetic carboxyl-functionalized attapulgite/calcium alginate beads	15:81	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.					
30172819	1	27	theme	simple	282:287	arg1	agitation					300:308	simple mechanical agitation	282:308	simple mechanical agitation	282:308	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	2	28	theme	adsorption	507:516	arg1	time					518:521	adsorption time	507:521	adsorption time	507:521	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	5	29	theme	6×	1040:1041	arg1	recycling					1053:1061	6× adsorbent recycling	1040:1061	6× adsorbent recycling	1040:1061	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	5	30	theme	removal	989:995	arg1	efficiency					997:1006	a Pb(II) removal efficiency	980:1006	a Pb(II) removal efficiency of >70%	980:1014	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	1	31	theme	magnetic	200:207	arg1	McAPT					246:250	McAPT	246:250	McAPT	246:250	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	1	31	theme	magnetic	200:207	arg1	attapulgite					233:243	magnetic carboxyl-functionalized attapulgite	200:243	magnetic carboxyl-functionalized attapulgite (McAPT)	200:251	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	0	32	theme	attapulgite/calcium	48:66	arg1	beads					77:81	magnetic carboxyl-functionalized attapulgite/calcium alginate beads	15:81	magnetic carboxyl-functionalized attapulgite/calcium alginate beads	15:81	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.					
30172819	1	33	theme	mechanical	289:298	arg1	agitation					300:308	simple mechanical agitation	282:308	simple mechanical agitation	282:308	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	2	34	theme	adsorbent	456:464	arg1	concentration					482:494	initial concentration	474:494	initial concentration of Pb(II), adsorption time, pH, and temperature	474:542	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	34	theme	adsorbent	456:464	arg1	dosage					466:471	adsorbent dosage	456:471	adsorbent dosage	456:471	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	5	35	theme	adsorbent	1043:1051	arg1	recycling					1053:1061	6× adsorbent recycling	1040:1061	6× adsorbent recycling	1040:1061	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	1	36	theme	carboxyl-functionalized	209:231	arg1	McAPT					246:250	McAPT	246:250	McAPT	246:250	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	1	36	theme	carboxyl-functionalized	209:231	arg1	attapulgite					233:243	magnetic carboxyl-functionalized attapulgite	200:243	magnetic carboxyl-functionalized attapulgite (McAPT)	200:251	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	5	37	theme	@	1115:1115	arg1	Alg					1116:1118	McAPT@Alg	1110:1118	McAPT@Alg	1110:1118	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	2	38	theme	temperature	532:542	arg1	concentration					482:494	initial concentration	474:494	initial concentration of Pb(II), adsorption time, pH, and temperature	474:542	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	38	theme	temperature	532:542	arg1	dosage					466:471	adsorbent dosage	456:471	adsorbent dosage	456:471	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	5	39	theme	contaminated	1145:1156	arg1	water					1158:1162	contaminated water	1145:1162	contaminated water	1145:1162	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	2	40	theme	novel	335:339	arg1	composite					351:359	The novel McAPT@Alg composite	331:359	The novel McAPT@Alg composite	331:359	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	0	41	theme	beads	77:81	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.	0:126	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.					
30172819	5	42	theme	%	1014:1014	arg1	efficiency					997:1006	a Pb(II) removal efficiency	980:1006	a Pb(II) removal efficiency of >70%	980:1014	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	4	43	theme	experimental	811:822	arg1	suitable					834:841	suitable	834:841	suitable	834:841	The experimental data were suitable for application to the Langmuir isotherm model, and the adsorption kinetics agreed with the pseudo-second-order model.					
30172819	4	43	theme	experimental	811:822	arg1	data					824:827	The experimental data	807:827	The experimental data	807:827	The experimental data were suitable for application to the Langmuir isotherm model, and the adsorption kinetics agreed with the pseudo-second-order model.					
30172819	1	44	theme	present	135:141	arg1	study					143:147	the present study	131:147	the present study	131:147	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	1	45	theme	room	313:316	arg1	temperature					318:328	room temperature	313:328	room temperature	313:328	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	1	46	from	attapulgite	233:243	arg1	adsorbent					185:193	adsorbent	185:193	adsorbent	185:193	In the present study, we synthesized an effective Pb(II) adsorbent from magnetic carboxyl-functionalized attapulgite (McAPT) and sodium alginate (Alg) by simple mechanical agitation at room temperature.					
30172819	5	47	theme	Pb	982:983	arg1	efficiency					997:1006	a Pb(II) removal efficiency	980:1006	a Pb(II) removal efficiency of >70%	980:1014	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	2	48	theme	pH	524:525	arg1	concentration					482:494	initial concentration	474:494	initial concentration of Pb(II), adsorption time, pH, and temperature	474:542	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	48	theme	pH	524:525	arg1	dosage					466:471	adsorbent dosage	456:471	adsorbent dosage	456:471	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	0	49	from	solutions	117:125	arg1	removal					96:102	lead ion removal	87:102	lead ion removal from aqueous solutions	87:125	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.					
30172819	0	50	theme	ion	92:94	arg1	removal					96:102	lead ion removal	87:102	lead ion removal from aqueous solutions	87:125	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.					
30172819	2	51	theme	instrumental	412:423	arg1	techniques					425:434	instrumental techniques	412:434	instrumental techniques	412:434	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	0	52	theme	lead	87:90	arg1	removal					96:102	lead ion removal	87:102	lead ion removal from aqueous solutions	87:125	Fabrication of magnetic carboxyl-functionalized attapulgite/calcium alginate beads for lead ion removal from aqueous solutions.					
30172819	5	53	theme	excellent	1087:1095	arg1	potential					1097:1105	the excellent potential	1083:1105	the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water	1083:1162	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30172819	2	54	theme	techniques	425:434	arg1	number					402:407	a number	400:407	a number of instrumental techniques	400:434	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	2	55	theme	Alg	347:349	arg1	composite					351:359	The novel McAPT@Alg composite	331:359	The novel McAPT@Alg composite	331:359	The novel McAPT@Alg composite was systematically characterized using a number of instrumental techniques, and the effects of adsorbent dosage, initial concentration of Pb(II), adsorption time, pH, and temperature on the adsorption capacity were investigated by performing batch experiments.					
30172819	5	56	theme	Alg	1116:1118	arg1	potential					1097:1105	the excellent potential	1083:1105	the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water	1083:1162	More importantly, a Pb(II) removal efficiency of >70% could be achieved after 6× adsorbent recycling, which demonstrated the excellent potential of McAPT@Alg for removing Pb(II) from contaminated water.					
30818019	5	0	theme	detailed	1124:1131	arg1	profile					1143:1149	a detailed lipogenic profile	1122:1149	a detailed lipogenic profile	1122:1149	Fractional synthetic rates for TAG-bound fatty acids and glycerol were quantified separately providing a detailed lipogenic profile.					
30818019	3	1	theme	high	670:673	arg1	diet					687:690	a control (low starch; LS) or experimental (high starch; HS) diet	626:690	diet	687:690	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	3	1	theme	high	670:673	arg1	HS					683:684	high starch; HS	670:684	high starch; HS	670:684	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	2	2	theme	carnivorous	273:283	arg1	fish					285:288	Farmed carnivorous fish	266:288	Farmed carnivorous fish under high carbohydrate diets	266:318	Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both.					
30818019	7	3	theme	FA	1342:1343	arg1	DNL					1325:1327	DNL	1325:1327	DNL of TAG-bound FA	1325:1343	In European seabass, HS promoted DNL of TAG-bound FA, in muscle and VAT.					
30818019	3	4	theme	experimental	656:667	arg1	diet					687:690	a control (low starch; LS) or experimental (high starch; HS) diet	626:690	diet	687:690	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	3	4	theme	experimental	656:667	arg1	HS					683:684	high starch; HS	670:684	high starch; HS	670:684	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	9	5	theme	2H-enriched	1557:1567	arg1	TAG					1569:1571	no 2H-enriched TAG	1554:1571	no 2H-enriched TAG	1554:1571	In Asian seabass, HS had no effect on muscle FA composition and lipogenic flux, with no 2H-enriched TAG being detected.					
30818019	2	6	theme	carbohydrate	301:312	arg1	diets					314:318	high carbohydrate diets	296:318	high carbohydrate diets	296:318	Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both.					
30818019	3	7	theme	European	548:555	arg1	seabass					609:615	European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass	548:615	European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass	548:615	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	1	8	theme	high	170:173	arg1	interest					175:182	high interest	170:182	high interest in substituting marine-derived with vegetable-based ingredients as energy source	170:263	In aquaculture, there is high interest in substituting marine-derived with vegetable-based ingredients as energy source.					
30818019	6	9	theme	lipogenic	1240:1248	arg1	fluxes					1250:1255	their lipogenic fluxes	1234:1255	their lipogenic fluxes	1234:1255	The FA profile differed substantially between muscle and VAT in both species, but their lipogenic fluxes revealed even greater differences.					
30818019	3	10	theme	Asian	584:588	arg1	seabass					609:615	European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass	548:615	European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass	548:615	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	0	11	from	metabolism	54:63	arg1	seabass					117:123	Asian seabass	111:123	Asian seabass (Lates calcarifer)	111:142	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	0	11	from	metabolism	54:63	arg1	calcarifer					132:141	calcarifer	132:141	calcarifer	132:141	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	0	11	from	metabolism	54:63	arg1	European					75:82	farmed European	68:82	farmed European (Dicentrarchus labrax)	68:105	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	0	11	from	metabolism	54:63	arg1	labrax					99:104	Dicentrarchus labrax	85:104	Dicentrarchus labrax	85:104	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	7	12	theme	European	1295:1302	arg1	seabass					1304:1310	European seabass	1295:1310	European seabass	1295:1310	In European seabass, HS promoted DNL of TAG-bound FA, in muscle and VAT.					
30818019	3	13	theme	deuterated	773:782	arg1	2H2O					790:793	deuterated water 2H2O	773:793	deuterated water 2H2O	773:793	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	4	14	theme	metabolic	847:855	arg1	steps					857:861	well-defined metabolic steps	834:861	well-defined metabolic steps	834:861	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	5	15	theme	lipogenic	1133:1141	arg1	profile					1143:1149	a detailed lipogenic profile	1122:1149	a detailed lipogenic profile	1122:1149	Fractional synthetic rates for TAG-bound fatty acids and glycerol were quantified separately providing a detailed lipogenic profile.					
30818019	11	16	theme	different	1863:1871	arg1	models					1878:1883	different fish models	1863:1883	different fish models	1863:1883	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	10	17	from	enhancement	1629:1639	arg1	fish					1658:1661	HS-fed fish	1651:1661	HS-fed fish	1651:1661	VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling.					
30818019	4	18	theme	2H-enrichment	903:915	arg1	TAG					898:900	TAG	898:900	TAG	898:900	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	4	18	theme	2H-enrichment	903:915	arg1	triacylglycerols					880:895	triacylglycerols	880:895	triacylglycerols (TAG) 2H-enrichment by 2HNMR	880:924	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	3	19	theme	water	784:788	arg1	2H2O					790:793	deuterated water 2H2O	773:793	deuterated water 2H2O	773:793	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	2	20	theme	pathways	452:459	arg1	promotion					429:437	promotion	429:437	promotion of lipogenic pathways	429:459	Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both.					
30818019	2	20	theme	pathways	452:459	arg1	retention/accumulation					405:426	increased lipid retention/accumulation	389:426	increased lipid retention/accumulation	389:426	Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both.					
30818019	5	21	theme	synthetic	1030:1038	arg1	rates					1040:1044	Fractional synthetic rates	1019:1044	Fractional synthetic rates for TAG-bound fatty acids and glycerol	1019:1083	Fractional synthetic rates for TAG-bound fatty acids and glycerol were quantified separately providing a detailed lipogenic profile.					
30818019	10	22	theme	TAG-bound	1679:1687	arg1	cycling					1698:1704	high TAG-bound glycerol cycling	1674:1704	high TAG-bound glycerol cycling	1674:1704	VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling.					
30818019	8	23	theme	muscle	1398:1403	arg1	glycerol					1415:1422	muscle TAG-bound glycerol	1398:1422	muscle TAG-bound glycerol	1398:1422	High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling.					
30818019	0	24	theme	farmed	68:73	arg1	European					75:82	farmed European	68:82	farmed European (Dicentrarchus labrax)	68:105	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	0	24	theme	farmed	68:73	arg1	labrax					99:104	Dicentrarchus labrax	85:104	Dicentrarchus labrax	85:104	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	8	25	located	found	1389:1393	arg1	glycerol					1415:1422	muscle TAG-bound glycerol	1398:1422	muscle TAG-bound glycerol	1398:1422	High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling.					
30818019	8	25	located	found	1389:1393	arg2	2H-enrichment					1370:1382	High 2H-enrichment	1365:1382	High 2H-enrichment also found in muscle TAG-bound glycerol	1365:1422	High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling.					
30818019	4	26	theme	triacylglycerols	880:895	arg1	analysis					868:875	analysis	868:875	analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR	868:924	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	0	27	from	Impact	0:5	arg1	metabolism					54:63	extrahepatic tissue lipid metabolism	28:63	extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer)	28:142	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	11	28	theme	fish	1873:1876	arg1	models					1878:1883	different fish models	1863:1883	different fish models	1863:1883	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	11	29	theme	fish	1761:1764	arg1	metabolism					1772:1781	fish lipid metabolism	1761:1781	fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models	1761:1883	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	6	30	theme	greater	1271:1277	arg1	differences					1279:1289	even greater differences	1266:1289	even greater differences	1266:1289	The FA profile differed substantially between muscle and VAT in both species, but their lipogenic fluxes revealed even greater differences.					
30818019	0	31	theme	extrahepatic	28:39	arg1	metabolism					54:63	extrahepatic tissue lipid metabolism	28:63	extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer)	28:142	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	3	32	dep	determine	483:491	arg1	to					480:481	to	480:481	to	480:481	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	4	33	theme	lipogenesis	956:966	arg1	evaluation					934:943	evaluation	934:943	evaluation of de novo lipogenesis (DNL)	934:972	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	9	34	theme	Asian	1472:1476	arg1	seabass					1478:1484	Asian seabass	1472:1484	Asian seabass	1472:1484	In Asian seabass, HS had no effect on muscle FA composition and lipogenic flux, with no 2H-enriched TAG being detected.					
30818019	11	35	theme	different	1786:1794	arg1	tissues					1796:1802	different tissues	1786:1802	different tissues	1786:1802	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	0	36	theme	dietary	10:16	arg1	starch					18:23	dietary starch	10:23	dietary starch	10:23	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	3	37	theme	tissue	522:527	arg1	response					497:504	the response	493:504	the response of extrahepatic tissue to dietary starch	493:545	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	2	38	theme	lipid	399:403	arg1	retention/accumulation					405:426	increased lipid retention/accumulation	389:426	increased lipid retention/accumulation	389:426	Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both.					
30818019	5	39	theme	fatty	1060:1064	arg1	acids					1066:1070	TAG-bound fatty acids	1050:1070	TAG-bound fatty acids	1050:1070	Fractional synthetic rates for TAG-bound fatty acids and glycerol were quantified separately providing a detailed lipogenic profile.					
30818019	4	40	theme	de	948:949	arg1	lipogenesis					956:966	de novo lipogenesis	948:966	de novo lipogenesis (DNL)	948:972	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	4	40	theme	de	948:949	arg1	DNL					969:971	DNL	969:971	DNL	969:971	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	0	41	theme	tissue	41:46	arg1	metabolism					54:63	extrahepatic tissue lipid metabolism	28:63	extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer)	28:142	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	4	42	theme	adipose	997:1003	arg1	VAT					1013:1015	VAT	1013:1015	VAT	1013:1015	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	4	42	theme	adipose	997:1003	arg1	tissue					1005:1010	visceral adipose tissue	988:1010	visceral adipose tissue (VAT)	988:1016	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	3	43	theme	starch	641:646	arg1	LS					649:650	low starch; LS	637:650	low starch; LS	637:650	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	3	43	theme	starch	641:646	arg1	control					628:634	a control (low starch; LS) or experimental (high starch; HS) diet	626:690	control	628:634	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	9	44	theme	muscle	1507:1512	arg1	composition					1517:1527	muscle FA composition	1507:1527	muscle FA composition	1507:1527	In Asian seabass, HS had no effect on muscle FA composition and lipogenic flux, with no 2H-enriched TAG being detected.					
30818019	11	45	theme	2H2O	1742:1745	arg1	use					1735:1737	the use	1731:1737	the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models	1731:1883	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	11	46	from	use	1856:1858	arg1	models					1878:1883	different fish models	1863:1883	different fish models	1863:1883	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	4	47	dep	de	948:949	arg1	novo					951:954	novo	951:954	novo	951:954	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	9	48	theme	lipogenic	1533:1541	arg1	flux					1543:1546	lipogenic flux	1533:1546	lipogenic flux	1533:1546	In Asian seabass, HS had no effect on muscle FA composition and lipogenic flux, with no 2H-enriched TAG being detected.					
30818019	3	49	theme	dietary	532:538	arg1	starch					540:545	dietary starch	532:545	dietary starch	532:545	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	1	50	theme	energy	251:256	arg1	source					258:263	energy source	251:263	energy source	251:263	In aquaculture, there is high interest in substituting marine-derived with vegetable-based ingredients as energy source.					
30818019	10	51	from	VAT	1589:1591	arg1	hand					1606:1609	the other hand	1596:1609	the other hand	1596:1609	VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling.					
30818019	3	52	theme	starch	675:680	arg1	diet					687:690	a control (low starch; LS) or experimental (high starch; HS) diet	626:690	diet	687:690	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	3	52	theme	starch	675:680	arg1	HS					683:684	high starch; HS	670:684	high starch; HS	670:684	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	2	53	theme	Farmed	266:271	arg1	fish					285:288	Farmed carnivorous fish	266:288	Farmed carnivorous fish under high carbohydrate diets	266:318	Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both.					
30818019	10	54	theme	HS-fed	1651:1656	arg1	fish					1658:1661	HS-fed fish	1651:1661	HS-fed fish	1651:1661	VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling.					
30818019	7	55	theme	TAG-bound	1332:1340	arg1	FA					1342:1343	TAG-bound FA	1332:1343	TAG-bound FA	1332:1343	In European seabass, HS promoted DNL of TAG-bound FA, in muscle and VAT.					
30818019	9	56	contain	had	1490:1492	arg1	HS					1487:1488	HS	1487:1488	HS	1487:1488	In Asian seabass, HS had no effect on muscle FA composition and lipogenic flux, with no 2H-enriched TAG being detected.					
30818019	9	56	contain	had	1490:1492	arg2	effect					1497:1502	no effect	1494:1502	no effect	1494:1502	In Asian seabass, HS had no effect on muscle FA composition and lipogenic flux, with no 2H-enriched TAG being detected.					
30818019	0	57	theme	Asian	111:115	arg1	seabass					117:123	Asian seabass	111:123	Asian seabass (Lates calcarifer)	111:142	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	0	57	theme	Asian	111:115	arg1	calcarifer					132:141	calcarifer	132:141	calcarifer	132:141	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	2	58	theme	high	296:299	arg1	diets					314:318	high carbohydrate diets	296:318	high carbohydrate diets	296:318	Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both.					
30818019	3	59	theme	Dicentrarchus	558:570	arg1	European					548:555	European	548:555	European	548:555	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	3	59	theme	Dicentrarchus	558:570	arg1	labrax					572:577	Dicentrarchus labrax	558:577	Dicentrarchus labrax	558:577	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	10	60	theme	high	1674:1677	arg1	cycling					1698:1704	high TAG-bound glycerol cycling	1674:1704	high TAG-bound glycerol cycling	1674:1704	VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling.					
30818019	8	61	theme	High	1365:1368	arg1	2H-enrichment					1370:1382	High 2H-enrichment	1365:1382	High 2H-enrichment also found in muscle TAG-bound glycerol	1365:1422	High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling.					
30818019	6	62	theme	FA	1156:1157	arg1	profile					1159:1165	The FA profile	1152:1165	The FA profile	1152:1165	The FA profile differed substantially between muscle and VAT in both species, but their lipogenic fluxes revealed even greater differences.					
30818019	11	63	from	metabolism	1772:1781	arg1	tissues					1796:1802	different tissues	1786:1802	different tissues	1786:1802	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	4	64	theme	2H-labelling	813:824	arg1	Incorporation					796:808	Incorporation	796:808	Incorporation of 2H-labelling	796:824	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	10	65	theme	DNL	1644:1646	arg1	enhancement					1629:1639	a strong enhancement	1620:1639	a strong enhancement of DNL in HS-fed fish	1620:1661	VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling.					
30818019	4	66	theme	well-defined	834:845	arg1	steps					857:861	well-defined metabolic steps	834:861	well-defined metabolic steps	834:861	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	3	67	dep	saltwater	749:757	arg1	enriched					759:766	enriched	759:766	to saltwater enriched with deuterated water 2H2O	746:793	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	10	68	theme	glycerol	1689:1696	arg1	cycling					1698:1704	high TAG-bound glycerol cycling	1674:1704	high TAG-bound glycerol cycling	1674:1704	VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling.					
30818019	8	69	theme	TAG-bound	1405:1413	arg1	glycerol					1415:1422	muscle TAG-bound glycerol	1398:1422	muscle TAG-bound glycerol	1398:1422	High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling.					
30818019	0	70	theme	Dicentrarchus	85:97	arg1	European					75:82	farmed European	68:82	farmed European (Dicentrarchus labrax)	68:105	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	0	70	theme	Dicentrarchus	85:97	arg1	labrax					99:104	Dicentrarchus labrax	85:104	Dicentrarchus labrax	85:104	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	5	71	theme	TAG-bound	1050:1058	arg1	acids					1066:1070	TAG-bound fatty acids	1050:1070	TAG-bound fatty acids	1050:1070	Fractional synthetic rates for TAG-bound fatty acids and glycerol were quantified separately providing a detailed lipogenic profile.					
30818019	8	72	theme	role	1446:1449	arg1	indicative					1428:1437	indicative	1428:1437	indicative	1428:1437	High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling.					
30818019	11	73	theme	lipid	1766:1770	arg1	metabolism					1772:1781	fish lipid metabolism	1761:1781	fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models	1761:1883	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	5	74	theme	Fractional	1019:1028	arg1	rates					1040:1044	Fractional synthetic rates	1019:1044	Fractional synthetic rates for TAG-bound fatty acids and glycerol	1019:1083	Fractional synthetic rates for TAG-bound fatty acids and glycerol were quantified separately providing a detailed lipogenic profile.					
30818019	2	75	theme	lipogenic	442:450	arg1	pathways					452:459	lipogenic pathways	442:459	lipogenic pathways	442:459	Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both.					
30818019	8	76	theme	lipid	1454:1458	arg1	cycling					1460:1466	lipid cycling	1454:1466	lipid cycling	1454:1466	High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling.					
30818019	0	77	theme	starch	18:23	arg1	Impact					0:5	Impact	0:5	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).	0:143	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	11	78	theme	different	1811:1819	arg1	conditions					1829:1838	different dietary conditions	1811:1838	different dietary conditions	1811:1838	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	10	79	theme	strong	1622:1627	arg1	enhancement					1629:1639	a strong enhancement	1620:1639	a strong enhancement of DNL in HS-fed fish	1620:1661	VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling.					
30818019	8	80	from	role	1446:1449	arg1	cycling					1460:1466	lipid cycling	1454:1466	lipid cycling	1454:1466	High 2H-enrichment also found in muscle TAG-bound glycerol was indicative of its role on lipid cycling.					
30818019	2	81	theme	increased	389:397	arg1	retention/accumulation					405:426	increased lipid retention/accumulation	389:426	increased lipid retention/accumulation	389:426	Farmed carnivorous fish under high carbohydrate diets tend to increase adiposity but it remains unclear if this happens by increased lipid retention/accumulation, promotion of lipogenic pathways, or both.					
30818019	0	82	theme	lipid	48:52	arg1	metabolism					54:63	extrahepatic tissue lipid metabolism	28:63	extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer)	28:142	Impact of dietary starch on extrahepatic tissue lipid metabolism in farmed European (Dicentrarchus labrax) and Asian seabass (Lates calcarifer).					
30818019	3	83	theme	low	637:639	arg1	LS					649:650	low starch; LS	637:650	low starch; LS	637:650	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	3	83	theme	low	637:639	arg1	control					628:634	a control (low starch; LS) or experimental (high starch; HS) diet	626:690	control	628:634	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	11	84	theme	dietary	1821:1827	arg1	conditions					1829:1838	different dietary conditions	1811:1838	different dietary conditions	1811:1838	This study consolidated the use of 2H2O as tracer for fish lipid metabolism in different tissues, under different dietary conditions and suitable to use in different fish models.					
30818019	4	85	theme	visceral	988:995	arg1	VAT					1013:1015	VAT	1013:1015	VAT	1013:1015	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	4	85	theme	visceral	988:995	arg1	tissue					1005:1010	visceral adipose tissue	988:1010	visceral adipose tissue (VAT)	988:1016	Incorporation of 2H-labelling follows well-defined metabolic steps, and analysis of triacylglycerols (TAG) 2H-enrichment by 2HNMR allowed evaluation of de novo lipogenesis (DNL) in muscle and visceral adipose tissue (VAT).					
30818019	3	86	theme	extrahepatic	509:520	arg1	tissue					522:527	extrahepatic tissue	509:527	extrahepatic tissue	509:527	In order to determine the response of extrahepatic tissue to dietary starch, European (Dicentrarchus labrax) and Asian (Lates calcarifer) seabass were fed a control (low starch; LS) or experimental (high starch; HS) diet, for at least 21 days and then transferred for 6 days to saltwater enriched with deuterated water 2H2O.					
30818019	9	87	theme	FA	1514:1515	arg1	composition					1517:1527	muscle FA composition	1507:1527	muscle FA composition	1507:1527	In Asian seabass, HS had no effect on muscle FA composition and lipogenic flux, with no 2H-enriched TAG being detected.					
30818019	10	88	theme	other	1600:1604	arg1	hand					1606:1609	the other hand	1596:1609	the other hand	1596:1609	VAT on the other hand revealed a strong enhancement of DNL in HS-fed fish along with high TAG-bound glycerol cycling.					
30818019	1	89	theme	vegetable-based	220:234	arg1	ingredients					236:246	vegetable-based ingredients	220:246	vegetable-based ingredients as energy source	220:263	In aquaculture, there is high interest in substituting marine-derived with vegetable-based ingredients as energy source.					
31373133	6	0	theme	protein	1425:1431	arg1	production					1433:1442	protein production	1425:1442	protein production due to the absence of O-mannosylation in the plant host	1425:1498	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	3	1	theme	host-specific	605:617	arg1	PTMs					653:656	PTMs	653:656	PTMs	653:656	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	3	1	theme	host-specific	605:617	arg1	modifications					638:650	extensive host-specific post-translational modifications	595:650	extensive host-specific post-translational modifications (PTMs)	595:657	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	8	2	theme	N. benthamiana	1825:1838	arg1	machinery					1812:1820	the glycosylation machinery	1794:1820	the glycosylation machinery of N. benthamiana	1794:1838	All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.					
31373133	1	3	theme	such	323:326	arg1	biomass					328:334	such biomass	323:334	such biomass	323:334	Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass.					
31373133	7	4	theme	full-length	1562:1572	arg1	TrCel7Arec					1574:1583	the purified full-length TrCel7Arec	1549:1583	the purified full-length TrCel7Arec	1549:1583	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	3	5	theme	extensive	595:603	arg1	PTMs					653:656	PTMs	653:656	PTMs	653:656	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	3	5	theme	extensive	595:603	arg1	modifications					638:650	extensive host-specific post-translational modifications	595:650	extensive host-specific post-translational modifications (PTMs)	595:657	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	5	6	from	PTMs	1125:1128	arg1	substrates					1182:1191	commercial and industrial substrates	1156:1191	commercial and industrial substrates	1156:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	7	7	theme	lower	1593:1597	arg1	activity					1609:1616	25% lower catalytic activity	1589:1616	25% lower catalytic activity	1589:1616	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	8	8	theme	glycosylation	1798:1810	arg1	machinery					1812:1820	the glycosylation machinery	1794:1820	the glycosylation machinery of N. benthamiana	1794:1838	All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.					
31373133	4	9	theme	PTMs	776:779	arg1	impact					760:765	the impact	756:765	the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme	756:911	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	5	10	theme	Nicotiana	938:946	arg1	benthamiana					948:958	Nicotiana benthamiana	938:958	Nicotiana benthamiana using a vacuum-based transient expression technology	938:1011	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	5	11	from	terms	1116:1120	arg1	substrates					1182:1191	commercial and industrial substrates	1156:1191	commercial and industrial substrates	1156:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	4	12	theme	enzyme	784:789	arg1	performance					791:801	enzyme performance	784:801	enzyme performance	784:801	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	2	13	theme	production	376:385	arg1	platform					387:394	a safe and cost-effective production platform	350:394	a safe and cost-effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels	350:490	Plants offer a safe and cost-effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels.					
31373133	8	14	theme	efficient	1886:1894	arg1	cellulases					1896:1905	efficient cellulases	1886:1905	efficient cellulases	1886:1905	All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.					
31373133	5	15	theme	vacuum-based	968:979	arg1	technology					1002:1011	a vacuum-based transient expression technology	966:1011	a vacuum-based transient expression technology	966:1011	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	6	16	theme	TrCel7Arec	1367:1376	arg1	region					1357:1362	the linker region	1346:1362	the linker region of TrCel7Arec	1346:1376	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	6	16	theme	TrCel7Arec	1367:1376	arg1	vulnerable					1382:1391	vulnerable	1382:1391	vulnerable	1382:1391	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	3	17	theme	functional	518:527	arg1	protein					529:535	intact and functional protein	507:535	intact and functional protein	507:535	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	3	18	theme	recombinant	714:724	arg1	enzymes					726:732	recombinant enzymes	714:732	recombinant enzymes	714:732	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	5	19	theme	expression	991:1000	arg1	technology					1002:1011	a vacuum-based transient expression technology	966:1011	a vacuum-based transient expression technology	966:1011	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	4	20	theme	major	824:828	arg1	TrCel7A					848:854	the major cellobiohydrolase TrCel7A	820:854	the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme	820:911	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	0	21	theme	post-translational	179:196	arg1	modifications					198:210	host-specific post-translational modifications	165:210	host-specific post-translational modifications	165:210	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	7	22	contain	had	1585:1587	arg2	activity					1609:1616	25% lower catalytic activity	1589:1616	25% lower catalytic activity	1589:1616	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	7	22	contain	had	1585:1587	arg1	TrCel7Arec					1574:1583	the purified full-length TrCel7Arec	1549:1583	the purified full-length TrCel7Arec	1549:1583	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	4	23	theme	TrCel7A	848:854	arg1	stability					807:815	stability	807:815	stability	807:815	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	4	23	theme	TrCel7A	848:854	arg1	performance					791:801	enzyme performance	784:801	enzyme performance	784:801	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	6	24	theme	protein	1321:1327	arg1	structure					1329:1337	protein structure	1321:1337	protein structure	1321:1337	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	7	25	theme	O-glycans	1733:1741	arg1	lack					1725:1728	lack	1725:1728	lack of O-glycans in TrCel7Arec	1725:1755	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	7	25	theme	O-glycans	1733:1741	arg1	N-glycans					1711:1719	larger N-glycans	1704:1719	larger N-glycans	1704:1719	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	0	26	from	limitations	116:126	arg1	plants					155:160	plants	155:160	plants	155:160	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	6	27	theme	native	1521:1526	arg1	protein					1528:1534	the native protein	1517:1534	the native protein	1517:1534	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	1	28	from	production	225:234	arg1	feedstocks					268:277	lignocellulose feedstocks	253:277	lignocellulose feedstocks	253:277	Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass.					
31373133	1	29	theme	Sustainable	213:223	arg1	production					225:234	Sustainable production	213:234	Sustainable production of biofuels from lignocellulose feedstocks	213:277	Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass.					
31373133	8	30	dep	All	1759:1761	arg1	all					1766:1768	all	1766:1768	all	1766:1768	All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.					
31373133	3	31	theme	protein	529:535	arg1	Production					493:502	Production	493:502	Production of intact and functional protein	493:535	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	3	31	theme	protein	529:535	arg1	prerequisite					542:553	a prerequisite	540:553	a prerequisite for large-scale protein production	540:588	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	0	32	theme	cellulase	131:139	arg1	production					141:150	cellulase production	131:150	cellulase production in plants by host-specific post-translational modifications	131:210	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	0	33	theme	Nicotiana	86:94	arg1	benthamiana					96:106	Nicotiana benthamiana	86:106	Nicotiana benthamiana	86:106	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	4	34	theme	Trichoderma	861:871	arg1	reesei					873:878	Trichoderma reesei	861:878	Trichoderma reesei	861:878	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	4	34	theme	Trichoderma	861:871	arg1	enzyme					906:911	an industrially relevant enzyme	881:911	an industrially relevant enzyme	881:911	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	0	35	theme	In-depth	0:7	arg1	characterization					9:24	In-depth characterization	0:24	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana	0:106	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	5	36	theme	native	1078:1083	arg1	TrCel7Anat					1100:1109	TrCel7Anat	1100:1109	TrCel7Anat	1100:1109	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	5	36	theme	native	1078:1083	arg1	enzyme					1092:1097	the native fungal enzyme	1074:1097	the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates	1074:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	0	37	theme	reesei	41:46	arg1	characterization					9:24	In-depth characterization	0:24	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana	0:106	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	7	38	from	N-glycans	1711:1719	arg1	TrCel7Arec					1746:1755	TrCel7Arec	1746:1755	TrCel7Arec	1746:1755	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	5	39	theme	activity	1144:1151	arg1	terms					1116:1120	terms	1116:1120	terms of PTMs and catalytic activity on commercial and industrial substrates	1116:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	7	40	from	lack	1725:1728	arg1	TrCel7Arec					1746:1755	TrCel7Arec	1746:1755	TrCel7Arec	1746:1755	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	7	41	theme	substrate-binding	1647:1663	arg1	properties					1665:1674	substrate-binding properties	1647:1674	substrate-binding properties	1647:1674	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	5	42	theme	commercial	1156:1165	arg1	substrates					1182:1191	commercial and industrial substrates	1156:1191	commercial and industrial substrates	1156:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	3	43	theme	protein	571:577	arg1	production					579:588	large-scale protein production	559:588	large-scale protein production	559:588	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	5	44	from	enzyme	1092:1097	arg1	terms					1116:1120	terms	1116:1120	terms of PTMs and catalytic activity on commercial and industrial substrates	1116:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	1	45	theme	cheap	290:294	arg1	enzymes					296:302	cheap enzymes	290:302	cheap enzymes for degradation of such biomass	290:334	Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass.					
31373133	3	46	theme	post-translational	619:636	arg1	PTMs					653:656	PTMs	653:656	PTMs	653:656	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	3	46	theme	post-translational	619:636	arg1	modifications					638:650	extensive host-specific post-translational modifications	595:650	extensive host-specific post-translational modifications (PTMs)	595:657	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	6	47	from	production	1433:1442	arg1	host					1495:1498	the plant host	1485:1498	the plant host	1485:1498	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	2	48	theme	industrial	433:442	arg1	enzymes					444:450	industrial enzymes	433:450	industrial enzymes boosting biomass conversion to biofuels	433:490	Plants offer a safe and cost-effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels.					
31373133	4	49	from	stability	807:815	arg1	reesei					873:878	Trichoderma reesei	861:878	Trichoderma reesei	861:878	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	4	49	from	stability	807:815	arg1	enzyme					906:911	an industrially relevant enzyme	881:911	an industrially relevant enzyme	881:911	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	7	50	theme	catalytic	1599:1607	arg1	activity					1609:1616	25% lower catalytic activity	1589:1616	25% lower catalytic activity	1589:1616	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	6	51	theme	N-terminal	1211:1220	arg1	glutamate					1222:1230	the N-terminal glutamate	1207:1230	the N-terminal glutamate of TrCel7Arec	1207:1244	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	2	52	theme	safe	352:355	arg1	platform					387:394	a safe and cost-effective production platform	350:394	a safe and cost-effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels	350:490	Plants offer a safe and cost-effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels.					
31373133	4	53	from	performance	791:801	arg1	reesei					873:878	Trichoderma reesei	861:878	Trichoderma reesei	861:878	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	4	53	from	performance	791:801	arg1	enzyme					906:911	an industrially relevant enzyme	881:911	an industrially relevant enzyme	881:911	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	1	54	theme	biomass	328:334	arg1	degradation					308:318	degradation	308:318	degradation of such biomass	308:334	Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass.					
31373133	6	55	theme	proteolytic	1396:1406	arg1	digestion					1408:1416	proteolytic digestion	1396:1416	proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host	1396:1498	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	7	56	theme	purified	1553:1560	arg1	TrCel7Arec					1574:1583	the purified full-length TrCel7Arec	1549:1583	the purified full-length TrCel7Arec	1549:1583	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	4	57	theme	plant	770:774	arg1	PTMs					776:779	plant PTMs	770:779	plant PTMs	770:779	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	3	58	theme	catalytic	676:684	arg1	properties					686:695	the catalytic properties	672:695	the catalytic properties	672:695	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	1	59	theme	biofuels	239:246	arg1	production					225:234	Sustainable production	213:234	Sustainable production of biofuels from lignocellulose feedstocks	213:277	Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass.					
31373133	0	60	dep	reesei	41:46	arg1	TrCel7A					66:72	cellobiohydrolase TrCel7A	48:72	Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana	29:106	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	2	61	theme	cost-effective	361:374	arg1	platform					387:394	a safe and cost-effective production platform	350:394	a safe and cost-effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels	350:490	Plants offer a safe and cost-effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels.					
31373133	4	62	from	impact	760:765	arg1	stability					807:815	stability	807:815	stability	807:815	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	4	62	from	impact	760:765	arg1	performance					791:801	enzyme performance	784:801	enzyme performance	784:801	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	1	63	theme	lignocellulose	253:266	arg1	feedstocks					268:277	lignocellulose feedstocks	253:277	lignocellulose feedstocks	253:277	Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass.					
31373133	0	64	theme	host-specific	165:177	arg1	modifications					198:210	host-specific post-translational modifications	165:210	host-specific post-translational modifications	165:210	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	5	65	from	activity	1144:1151	arg1	substrates					1182:1191	commercial and industrial substrates	1156:1191	commercial and industrial substrates	1156:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	5	66	theme	transient	981:989	arg1	technology					1002:1011	a vacuum-based transient expression technology	966:1011	a vacuum-based transient expression technology	966:1011	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	8	67	theme	cellulases	1896:1905	arg1	production					1872:1881	the production	1868:1881	the production of efficient cellulases	1868:1905	All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.					
31373133	0	68	from	production	141:150	arg1	plants					155:160	plants	155:160	plants	155:160	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	6	69	theme	linker	1350:1355	arg1	region					1357:1362	the linker region	1346:1362	the linker region of TrCel7Arec	1346:1376	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	6	69	theme	linker	1350:1355	arg1	vulnerable					1382:1391	vulnerable	1382:1391	vulnerable	1382:1391	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	5	70	theme	industrial	1171:1180	arg1	substrates					1182:1191	commercial and industrial substrates	1156:1191	commercial and industrial substrates	1156:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	1	71	from	feedstocks	268:277	arg1	biofuels					239:246	biofuels	239:246	biofuels from lignocellulose feedstocks	239:277	Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass.					
31373133	1	71	from	feedstocks	268:277	arg1	production					225:234	Sustainable production	213:234	Sustainable production of biofuels from lignocellulose feedstocks	213:277	Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass.					
31373133	4	72	theme	cellobiohydrolase	830:846	arg1	TrCel7A					848:854	the major cellobiohydrolase TrCel7A	820:854	the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme	820:911	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	5	73	from	substrates	1182:1191	arg1	terms					1116:1120	terms	1116:1120	terms of PTMs and catalytic activity on commercial and industrial substrates	1116:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	3	74	theme	enzymes	726:732	arg1	stability					701:709	stability	701:709	stability	701:709	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	3	74	theme	enzymes	726:732	arg1	properties					686:695	the catalytic properties	672:695	the catalytic properties	672:695	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	5	75	theme	recombinant	1023:1033	arg1	TrCel7Arec					1043:1052	TrCel7Arec	1043:1052	TrCel7Arec	1043:1052	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	5	75	theme	recombinant	1023:1033	arg1	enzyme					1035:1040	this recombinant enzyme	1018:1040	this recombinant enzyme (TrCel7Arec )	1018:1054	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	7	76	theme	larger	1704:1709	arg1	N-glycans					1711:1719	larger N-glycans	1704:1719	larger N-glycans	1704:1719	In general, the purified full-length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate-binding properties, which can be attributed to larger N-glycans and lack of O-glycans in TrCel7Arec .					
31373133	0	77	theme	production	141:150	arg1	limitations					116:126	limitations	116:126	limitations of cellulase production in plants by host-specific post-translational modifications	116:210	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	4	78	theme	relevant	897:904	arg1	reesei					873:878	Trichoderma reesei	861:878	Trichoderma reesei	861:878	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	4	78	theme	relevant	897:904	arg1	enzyme					906:911	an industrially relevant enzyme	881:911	an industrially relevant enzyme	881:911	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	4	79	from	reesei	873:878	arg1	stability					807:815	stability	807:815	stability	807:815	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	4	79	from	reesei	873:878	arg1	performance					791:801	enzyme performance	784:801	enzyme performance	784:801	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	4	79	from	reesei	873:878	arg1	TrCel7A					848:854	the major cellobiohydrolase TrCel7A	820:854	the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme	820:911	Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme.					
31373133	6	80	theme	O-mannosylation	1466:1480	arg1	absence					1455:1461	the absence	1451:1461	the absence of O-mannosylation	1451:1480	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	2	81	theme	biomass	461:467	arg1	conversion					469:478	biomass conversion	461:478	biomass conversion to biofuels	461:490	Plants offer a safe and cost-effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels.					
31373133	5	82	theme	fungal	1085:1090	arg1	TrCel7Anat					1100:1109	TrCel7Anat	1100:1109	TrCel7Anat	1100:1109	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	5	82	theme	fungal	1085:1090	arg1	enzyme					1092:1097	the native fungal enzyme	1074:1097	the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates	1074:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	3	83	theme	large-scale	559:569	arg1	production					579:588	large-scale protein production	559:588	large-scale protein production	559:588	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	0	84	theme	cellobiohydrolase	48:64	arg1	TrCel7A					66:72	cellobiohydrolase TrCel7A	48:72	Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana	29:106	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31373133	5	85	theme	catalytic	1134:1142	arg1	activity					1144:1151	catalytic activity	1134:1151	catalytic activity	1134:1151	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	3	86	theme	intact	507:512	arg1	protein					529:535	intact and functional protein	507:535	intact and functional protein	507:535	Production of intact and functional protein is a prerequisite for large-scale protein production, and extensive host-specific post-translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes.					
31373133	6	87	theme	plant	1489:1493	arg1	host					1495:1498	the plant host	1485:1498	the plant host	1485:1498	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	5	88	theme	PTMs	1125:1128	arg1	terms					1116:1120	terms	1116:1120	terms of PTMs and catalytic activity on commercial and industrial substrates	1116:1191	TrCel7A was produced in Nicotiana benthamiana using a vacuum-based transient expression technology, and this recombinant enzyme (TrCel7Arec ) was compared with the native fungal enzyme (TrCel7Anat ) in terms of PTMs and catalytic activity on commercial and industrial substrates.					
31373133	6	89	theme	TrCel7Arec	1235:1244	arg1	glutamate					1222:1230	the N-terminal glutamate	1207:1230	the N-terminal glutamate of TrCel7Arec	1207:1244	We show that the N-terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O-mannosylation in the plant host as compared with the native protein.					
31373133	0	90	from	plants	155:160	arg1	limitations					116:126	limitations	116:126	limitations of cellulase production in plants by host-specific post-translational modifications	116:210	In-depth characterization of Trichoderma reesei cellobiohydrolase TrCel7A produced in Nicotiana benthamiana reveals limitations of cellulase production in plants by host-specific post-translational modifications.					
31444661	8	0	theme	Ex	958:959	arg1	permeability					966:977	Ex vivo permeability	958:977	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans	958:1072	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans were examined.					
31444661	3	1	theme	hyaluronic	524:533	arg1	gel					546:548	hyaluronic acid-based gel	524:548	hyaluronic acid-based gel	524:548	The study aims to formulate MZ self-nanoemulsion (MZ-NE) and combine it within hyaluronic acid-based gel.					
31444661	1	2	theme	dissolution	243:253	arg1	properties					255:264	limited dissolution properties	235:264	limited dissolution properties	235:264	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	13	3	theme	oral	1764:1767	arg1	pharmacotherapy					1776:1790	oral thrush pharmacotherapy	1764:1790	oral thrush pharmacotherapy	1764:1790	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	6	4	theme	gel	893:895	arg1	base					897:900	a hyaluronic acid gel base	875:900	a hyaluronic acid gel base	875:900	Further, the optimized MZ-NE was loaded into a hyaluronic acid gel base.					
31444661	8	5	theme	optimized	982:990	arg1	formulation					992:1002	optimized formulation	982:1002	optimized formulation	982:1002	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans were examined.					
31444661	6	6	theme	hyaluronic	877:886	arg1	base					897:900	a hyaluronic acid gel base	875:900	a hyaluronic acid gel base	875:900	Further, the optimized MZ-NE was loaded into a hyaluronic acid gel base.					
31444661	2	7	theme	system-based	331:342	arg1	gel					344:346	Self-nanoemulsifying delivery system-based gel	301:346	Self-nanoemulsifying delivery system-based gel of MZ	301:352	Self-nanoemulsifying delivery system-based gel of MZ can improve both solubility and oral mucosal absorption with enhanced antifungal activity.					
31444661	2	8	theme	Self-nanoemulsifying	301:320	arg1	gel					344:346	Self-nanoemulsifying delivery system-based gel	301:346	Self-nanoemulsifying delivery system-based gel of MZ	301:352	Self-nanoemulsifying delivery system-based gel of MZ can improve both solubility and oral mucosal absorption with enhanced antifungal activity.					
31444661	1	9	theme	class	184:188	arg1	drug					225:228	a BCS class II antifungal poorly water-soluble drug	178:228	a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects	178:298	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	1	9	theme	class	184:188	arg1	nitrate					162:168	Miconazole nitrate	151:168	Miconazole nitrate (MZ)	151:173	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	13	10	theme	better	1708:1713	arg1	activity					1726:1733	better antifungal activity	1708:1733	better antifungal activity	1708:1733	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	5	11	theme	formulation	728:738	arg1	variable					740:747	a formulation variable	726:747	a formulation variable	726:747	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	5	11	theme	formulation	728:738	arg1	levels					699:704	the levels	695:704	the levels of NE components	695:721	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	1	12	theme	antifungal	193:202	arg1	drug					225:228	a BCS class II antifungal poorly water-soluble drug	178:228	a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects	178:298	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	1	12	theme	antifungal	193:202	arg1	nitrate					162:168	Miconazole nitrate	151:168	Miconazole nitrate (MZ)	151:173	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	11	13	theme	Pseudoplastic	1329:1341	arg1	flow					1343:1346	Pseudoplastic flow	1329:1346	Pseudoplastic flow with thixotropic behavior	1329:1372	Pseudoplastic flow with thixotropic behavior was observed, which is desirable for oral gels.					
31444661	8	14	dep	Ex	958:959	arg1	vivo					961:964	vivo	961:964	vivo	961:964	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans were examined.					
31444661	5	15	theme	NE	709:710	arg1	components					712:721	NE components	709:721	NE components	709:721	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	5	16	from	effects	764:770	arg1	zones					823:827	antifungal inhibition zones	801:827	antifungal inhibition zones	801:827	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	5	16	from	effects	764:770	arg1	size					792:795	the mean globule size	775:795	the mean globule size	775:795	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	12	17	dep	ex	1465:1466	arg1	vivo					1468:1471	vivo	1468:1471	vivo	1468:1471	The optimized formulation exhibited higher ex vivo skin permeability and enhanced antifungal activity by 1.85 and 2.179, respectively, compared to MZ-SNEDDS, and by 1.52 and 1.72 folds, respectively, compared to marketed gel.					
31444661	0	18	theme	Acid-Based	56:65	arg1	Hydrogel					67:74	Hyaluronic Acid-Based Hydrogel	45:74	Hyaluronic Acid-Based Hydrogel	45:74	Preparation, Optimization, and Evaluation of Hyaluronic Acid-Based Hydrogel Loaded with Miconazole Self-Nanoemulsion for the Treatment of Oral Thrush.					
31444661	1	19	theme	Miconazole	151:160	arg1	drug					225:228	a BCS class II antifungal poorly water-soluble drug	178:228	a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects	178:298	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	1	19	theme	Miconazole	151:160	arg1	nitrate					162:168	Miconazole nitrate	151:168	Miconazole nitrate (MZ)	151:173	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	13	20	theme	hyaluronic	1664:1673	arg1	gel					1691:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	5	21	theme	mean	779:782	arg1	size					792:795	the mean globule size	775:795	the mean globule size	775:795	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	8	22	theme	sheep	1028:1032	arg1	mucous					1018:1023	buccal mucous	1011:1023	buccal mucous of sheep and inhibition against Candida albicans	1011:1072	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans were examined.					
31444661	13	23	theme	oral	1686:1689	arg1	gel					1691:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	8	24	theme	inhibition	1038:1047	arg1	mucous					1018:1023	buccal mucous	1011:1023	buccal mucous of sheep and inhibition against Candida albicans	1011:1072	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans were examined.					
31444661	11	25	with	flow	1343:1346	arg1	behavior					1365:1372	thixotropic behavior	1353:1372	thixotropic behavior	1353:1372	Pseudoplastic flow with thixotropic behavior was observed, which is desirable for oral gels.					
31444661	9	26	theme	Mixture	1089:1095	arg1	design					1097:1102	Mixture design	1089:1102	Mixture design	1089:1102	Mixture design was used to optimize the composition of MZ-NE formulation as 22, 67, and 10% for clove oil, Labrasol, and propylene glycol, respectively.					
31444661	2	27	theme	enhanced	415:422	arg1	activity					435:442	enhanced antifungal activity	415:442	enhanced antifungal activity	415:442	Self-nanoemulsifying delivery system-based gel of MZ can improve both solubility and oral mucosal absorption with enhanced antifungal activity.					
31444661	8	28	theme	Candida	1057:1063	arg1	albicans					1065:1072	Candida albicans	1057:1072	Candida albicans	1057:1072	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans were examined.					
31444661	7	29	theme	gel	940:942	arg1	behavior					915:922	Rheological behavior	903:922	Rheological behavior of the prepared gel	903:942	Rheological behavior of the prepared gel was assessed.					
31444661	12	30	theme	marketed	1634:1641	arg1	gel					1643:1645	marketed gel	1634:1645	marketed gel	1634:1645	The optimized formulation exhibited higher ex vivo skin permeability and enhanced antifungal activity by 1.85 and 2.179, respectively, compared to MZ-SNEDDS, and by 1.52 and 1.72 folds, respectively, compared to marketed gel.					
31444661	13	31	theme	Optimized	1648:1656	arg1	gel					1691:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	1	32	dep	nitrate	162:168	arg1	MZ					171:172	MZ	171:172	MZ	171:172	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	11	33	theme	oral	1411:1414	arg1	gels					1416:1419	oral gels	1411:1419	oral gels	1411:1419	Pseudoplastic flow with thixotropic behavior was observed, which is desirable for oral gels.					
31444661	1	34	theme	gastrointestinal	270:285	arg1	effects					292:298	gastrointestinal side effects	270:298	gastrointestinal side effects	270:298	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	4	35	theme	various	568:574	arg1	oils					576:579	various oils	568:579	various oils	568:579	MZ solubility in various oils, surfactants, and cosurfactant used in NE formulations were evaluated.					
31444661	10	36	theme	globule	1278:1284	arg1	size					1286:1289	globule size	1278:1289	globule size of 113 nm	1278:1299	The optimized formulation indicated globule size of 113 nm with 29 mm inhibition zone.					
31444661	3	37	theme	MZ	473:474	arg1	self-nanoemulsion					476:492	MZ self-nanoemulsion	473:492	MZ self-nanoemulsion (MZ-NE)	473:500	The study aims to formulate MZ self-nanoemulsion (MZ-NE) and combine it within hyaluronic acid-based gel.					
31444661	3	37	theme	MZ	473:474	arg1	MZ-NE					495:499	MZ-NE	495:499	MZ-NE	495:499	The study aims to formulate MZ self-nanoemulsion (MZ-NE) and combine it within hyaluronic acid-based gel.					
31444661	4	38	from	solubility	554:563	arg1	cosurfactant					599:610	cosurfactant	599:610	cosurfactant	599:610	MZ solubility in various oils, surfactants, and cosurfactant used in NE formulations were evaluated.					
31444661	4	38	from	solubility	554:563	arg1	oils					576:579	various oils	568:579	various oils	568:579	MZ solubility in various oils, surfactants, and cosurfactant used in NE formulations were evaluated.					
31444661	4	38	from	solubility	554:563	arg1	surfactants					582:592	surfactants	582:592	surfactants	582:592	MZ solubility in various oils, surfactants, and cosurfactant used in NE formulations were evaluated.					
31444661	12	39	theme	antifungal	1504:1513	arg1	activity					1515:1522	enhanced antifungal activity	1495:1522	enhanced antifungal activity	1495:1522	The optimized formulation exhibited higher ex vivo skin permeability and enhanced antifungal activity by 1.85 and 2.179, respectively, compared to MZ-SNEDDS, and by 1.52 and 1.72 folds, respectively, compared to marketed gel.					
31444661	10	40	theme	inhibition	1312:1321	arg1	zone					1323:1326	29 mm inhibition zone	1306:1326	29 mm inhibition zone	1306:1326	The optimized formulation indicated globule size of 113 nm with 29 mm inhibition zone.					
31444661	5	41	theme	antifungal	801:810	arg1	zones					823:827	antifungal inhibition zones	801:827	antifungal inhibition zones	801:827	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	9	42	theme	formulation	1150:1160	arg1	composition					1129:1139	the composition	1125:1139	the composition of MZ-NE formulation	1125:1160	Mixture design was used to optimize the composition of MZ-NE formulation as 22, 67, and 10% for clove oil, Labrasol, and propylene glycol, respectively.					
31444661	2	43	theme	MZ	351:352	arg1	gel					344:346	Self-nanoemulsifying delivery system-based gel	301:346	Self-nanoemulsifying delivery system-based gel of MZ	301:352	Self-nanoemulsifying delivery system-based gel of MZ can improve both solubility and oral mucosal absorption with enhanced antifungal activity.					
31444661	12	44	theme	skin	1473:1476	arg1	permeability					1478:1489	higher ex vivo skin permeability	1458:1489	higher ex vivo skin permeability	1458:1489	The optimized formulation exhibited higher ex vivo skin permeability and enhanced antifungal activity by 1.85 and 2.179, respectively, compared to MZ-SNEDDS, and by 1.52 and 1.72 folds, respectively, compared to marketed gel.					
31444661	1	45	with	drug	225:228	arg1	effects					292:298	gastrointestinal side effects	270:298	gastrointestinal side effects	270:298	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	1	45	with	drug	225:228	arg1	properties					255:264	limited dissolution properties	235:264	limited dissolution properties	235:264	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	3	46	theme	acid-based	535:544	arg1	gel					546:548	hyaluronic acid-based gel	524:548	hyaluronic acid-based gel	524:548	The study aims to formulate MZ self-nanoemulsion (MZ-NE) and combine it within hyaluronic acid-based gel.					
31444661	7	47	theme	Rheological	903:913	arg1	behavior					915:922	Rheological behavior	903:922	Rheological behavior of the prepared gel	903:942	Rheological behavior of the prepared gel was assessed.					
31444661	12	48	theme	optimized	1426:1434	arg1	formulation					1436:1446	The optimized formulation	1422:1446	The optimized formulation	1422:1446	The optimized formulation exhibited higher ex vivo skin permeability and enhanced antifungal activity by 1.85 and 2.179, respectively, compared to MZ-SNEDDS, and by 1.52 and 1.72 folds, respectively, compared to marketed gel.					
31444661	11	49	theme	thixotropic	1353:1363	arg1	behavior					1365:1372	thixotropic behavior	1353:1372	thixotropic behavior	1353:1372	Pseudoplastic flow with thixotropic behavior was observed, which is desirable for oral gels.					
31444661	1	50	theme	limited	235:241	arg1	dissolution					243:253	limited dissolution	235:253	limited dissolution properties	235:264	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	8	51	theme	formulation	992:1002	arg1	permeability					966:977	Ex vivo permeability	958:977	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans	958:1072	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans were examined.					
31444661	13	52	theme	thrush	1769:1774	arg1	pharmacotherapy					1776:1790	oral thrush pharmacotherapy	1764:1790	oral thrush pharmacotherapy	1764:1790	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	12	53	dep	1.52	1587:1590	arg1	folds					1601:1605	folds	1601:1605	folds	1601:1605	The optimized formulation exhibited higher ex vivo skin permeability and enhanced antifungal activity by 1.85 and 2.179, respectively, compared to MZ-SNEDDS, and by 1.52 and 1.72 folds, respectively, compared to marketed gel.					
31444661	6	54	theme	acid	888:891	arg1	base					897:900	a hyaluronic acid gel base	875:900	a hyaluronic acid gel base	875:900	Further, the optimized MZ-NE was loaded into a hyaluronic acid gel base.					
31444661	8	55	theme	buccal	1011:1016	arg1	mucous					1018:1023	buccal mucous	1011:1023	buccal mucous of sheep and inhibition against Candida albicans	1011:1072	Ex vivo permeability of optimized formulation across buccal mucous of sheep and inhibition against Candida albicans were examined.					
31444661	5	56	theme	Mixture	652:658	arg1	design					660:665	Mixture design	652:665	Mixture design	652:665	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	9	57	theme	clove	1185:1189	arg1	oil					1191:1193	clove oil	1185:1193	clove oil	1185:1193	Mixture design was used to optimize the composition of MZ-NE formulation as 22, 67, and 10% for clove oil, Labrasol, and propylene glycol, respectively.					
31444661	9	58	used	used	1108:1111	arg2	design					1097:1102	Mixture design	1089:1102	Mixture design	1089:1102	Mixture design was used to optimize the composition of MZ-NE formulation as 22, 67, and 10% for clove oil, Labrasol, and propylene glycol, respectively.					
31444661	5	59	theme	components	712:721	arg1	variable					740:747	a formulation variable	726:747	a formulation variable	726:747	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	5	59	theme	components	712:721	arg1	levels					699:704	the levels	695:704	the levels of NE components	695:721	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	2	60	theme	delivery	322:329	arg1	gel					344:346	Self-nanoemulsifying delivery system-based gel	301:346	Self-nanoemulsifying delivery system-based gel of MZ	301:352	Self-nanoemulsifying delivery system-based gel of MZ can improve both solubility and oral mucosal absorption with enhanced antifungal activity.					
31444661	1	61	theme	BCS	180:182	arg1	drug					225:228	a BCS class II antifungal poorly water-soluble drug	178:228	a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects	178:298	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	1	61	theme	BCS	180:182	arg1	nitrate					162:168	Miconazole nitrate	151:168	Miconazole nitrate (MZ)	151:173	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	9	62	theme	MZ-NE	1144:1148	arg1	formulation					1150:1160	MZ-NE formulation	1144:1160	MZ-NE formulation	1144:1160	Mixture design was used to optimize the composition of MZ-NE formulation as 22, 67, and 10% for clove oil, Labrasol, and propylene glycol, respectively.					
31444661	0	63	theme	Thrush	143:148	arg1	Treatment					125:133	the Treatment	121:133	the Treatment of Oral Thrush	121:148	Preparation, Optimization, and Evaluation of Hyaluronic Acid-Based Hydrogel Loaded with Miconazole Self-Nanoemulsion for the Treatment of Oral Thrush.					
31444661	13	64	theme	antifungal	1715:1724	arg1	activity					1726:1733	better antifungal activity	1708:1733	better antifungal activity	1708:1733	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	9	65	theme	propylene	1210:1218	arg1	glycol					1220:1225	propylene glycol	1210:1225	propylene glycol	1210:1225	Mixture design was used to optimize the composition of MZ-NE formulation as 22, 67, and 10% for clove oil, Labrasol, and propylene glycol, respectively.					
31444661	6	66	theme	optimized	843:851	arg1	MZ-NE					853:857	the optimized MZ-NE	839:857	the optimized MZ-NE	839:857	Further, the optimized MZ-NE was loaded into a hyaluronic acid gel base.					
31444661	0	67	theme	Hydrogel	67:74	arg1	Optimization					13:24	Optimization	13:24	Optimization	13:24	Preparation, Optimization, and Evaluation of Hyaluronic Acid-Based Hydrogel Loaded with Miconazole Self-Nanoemulsion for the Treatment of Oral Thrush.					
31444661	0	67	theme	Hydrogel	67:74	arg1	Evaluation					31:40	Evaluation	31:40	Evaluation	31:40	Preparation, Optimization, and Evaluation of Hyaluronic Acid-Based Hydrogel Loaded with Miconazole Self-Nanoemulsion for the Treatment of Oral Thrush.					
31444661	0	67	theme	Hydrogel	67:74	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, Optimization, and Evaluation of Hyaluronic Acid-Based Hydrogel Loaded with Miconazole Self-Nanoemulsion for the Treatment of Oral Thrush.					
31444661	12	68	dep	higher	1458:1463	arg1	ex					1465:1466	ex	1465:1466	ex	1465:1466	The optimized formulation exhibited higher ex vivo skin permeability and enhanced antifungal activity by 1.85 and 2.179, respectively, compared to MZ-SNEDDS, and by 1.52 and 1.72 folds, respectively, compared to marketed gel.					
31444661	13	69	theme	acid-based	1675:1684	arg1	gel					1691:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	0	70	theme	Self-Nanoemulsion	99:115	arg1	Miconazole					88:97	Miconazole	88:97	Miconazole Self-Nanoemulsion for the Treatment of Oral Thrush	88:148	Preparation, Optimization, and Evaluation of Hyaluronic Acid-Based Hydrogel Loaded with Miconazole Self-Nanoemulsion for the Treatment of Oral Thrush.					
31444661	13	71	from	potential	1751:1759	arg1	pharmacotherapy					1776:1790	oral thrush pharmacotherapy	1764:1790	oral thrush pharmacotherapy	1764:1790	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	10	72	theme	optimized	1246:1254	arg1	formulation					1256:1266	The optimized formulation	1242:1266	The optimized formulation	1242:1266	The optimized formulation indicated globule size of 113 nm with 29 mm inhibition zone.					
31444661	5	73	theme	inhibition	812:821	arg1	zones					823:827	antifungal inhibition zones	801:827	antifungal inhibition zones	801:827	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	13	74	theme	MZ-NE	1658:1662	arg1	gel					1691:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel	1648:1693	Optimized MZ-NE hyaluronic acid-based oral gel demonstrated better antifungal activity, indicating its potential in oral thrush pharmacotherapy.					
31444661	10	75	theme	113 nm	1294:1299	arg1	size					1286:1289	globule size	1278:1289	globule size of 113 nm	1278:1299	The optimized formulation indicated globule size of 113 nm with 29 mm inhibition zone.					
31444661	2	76	theme	mucosal	391:397	arg1	absorption					399:408	oral mucosal absorption	386:408	oral mucosal absorption	386:408	Self-nanoemulsifying delivery system-based gel of MZ can improve both solubility and oral mucosal absorption with enhanced antifungal activity.					
31444661	1	77	theme	side	287:290	arg1	effects					292:298	gastrointestinal side effects	270:298	gastrointestinal side effects	270:298	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	7	78	theme	prepared	931:938	arg1	gel					940:942	the prepared gel	927:942	the prepared gel	927:942	Rheological behavior of the prepared gel was assessed.					
31444661	2	79	theme	oral	386:389	arg1	absorption					399:408	oral mucosal absorption	386:408	oral mucosal absorption	386:408	Self-nanoemulsifying delivery system-based gel of MZ can improve both solubility and oral mucosal absorption with enhanced antifungal activity.					
31444661	5	80	theme	globule	784:790	arg1	size					792:795	the mean globule size	775:795	the mean globule size	775:795	Mixture design was implemented to optimize the levels of NE components as a formulation variable to study their effects on the mean globule size and antifungal inhibition zones.					
31444661	4	81	theme	MZ	551:552	arg1	solubility					554:563	MZ solubility	551:563	MZ solubility in various oils, surfactants, and cosurfactant used in NE formulations	551:634	MZ solubility in various oils, surfactants, and cosurfactant used in NE formulations were evaluated.					
31444661	2	82	theme	antifungal	424:433	arg1	activity					435:442	enhanced antifungal activity	415:442	enhanced antifungal activity	415:442	Self-nanoemulsifying delivery system-based gel of MZ can improve both solubility and oral mucosal absorption with enhanced antifungal activity.					
31444661	0	83	theme	Hyaluronic	45:54	arg1	Hydrogel					67:74	Hyaluronic Acid-Based Hydrogel	45:74	Hyaluronic Acid-Based Hydrogel	45:74	Preparation, Optimization, and Evaluation of Hyaluronic Acid-Based Hydrogel Loaded with Miconazole Self-Nanoemulsion for the Treatment of Oral Thrush.					
31444661	12	84	theme	enhanced	1495:1502	arg1	activity					1515:1522	enhanced antifungal activity	1495:1522	enhanced antifungal activity	1495:1522	The optimized formulation exhibited higher ex vivo skin permeability and enhanced antifungal activity by 1.85 and 2.179, respectively, compared to MZ-SNEDDS, and by 1.52 and 1.72 folds, respectively, compared to marketed gel.					
31444661	10	85	theme	29 mm	1306:1310	arg1	zone					1323:1326	29 mm inhibition zone	1306:1326	29 mm inhibition zone	1306:1326	The optimized formulation indicated globule size of 113 nm with 29 mm inhibition zone.					
31444661	12	86	theme	higher	1458:1463	arg1	permeability					1478:1489	higher ex vivo skin permeability	1458:1489	higher ex vivo skin permeability	1458:1489	The optimized formulation exhibited higher ex vivo skin permeability and enhanced antifungal activity by 1.85 and 2.179, respectively, compared to MZ-SNEDDS, and by 1.52 and 1.72 folds, respectively, compared to marketed gel.					
31444661	4	87	theme	NE	620:621	arg1	formulations					623:634	NE formulations	620:634	NE formulations	620:634	MZ solubility in various oils, surfactants, and cosurfactant used in NE formulations were evaluated.					
31444661	1	88	theme	water-soluble	211:223	arg1	drug					225:228	a BCS class II antifungal poorly water-soluble drug	178:228	a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects	178:298	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
31444661	1	88	theme	water-soluble	211:223	arg1	nitrate					162:168	Miconazole nitrate	151:168	Miconazole nitrate (MZ)	151:173	Miconazole nitrate (MZ) is a BCS class II antifungal poorly water-soluble drug with limited dissolution properties and gastrointestinal side effects.					
30447369	6	0	theme	component	1232:1240	arg1	mixture					1242:1248	the single component mixture	1221:1248	the single component mixture	1221:1248	Batch regeneration of the spent adsorbent from the single component mixture was effective.					
30447369	7	1	theme	0.0	1353:1355	arg1	%					1356:1356	%	1356:1356	%	1356:1356	Multiple adsorption/desorption experiments were also carried out, and it was found that 0.0% mass loss of the G/ECH-CS was observed after the third cycle of adsorption/desorption studies.					
30447369	2	2	theme	functionalized	362:375	arg1	materials					377:385	the functionalized materials	358:385	the functionalized materials	358:385	In this study the functionalized materials were characterize by FTIR, SEM/EDX, TGA/DTA, BET and XRD.					
30447369	5	3	theme	Cd	1068:1069	arg1	ions					1075:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	0	4	theme	chitosan/epichlorohydrin	71:94	arg1	composite					96:104	chitosan/epichlorohydrin composite	71:104	chitosan/epichlorohydrin composite	71:104	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	3	5	theme	batch	670:674	arg1	applications					676:687	batch applications	670:687	batch applications	670:687	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	1	6	from	wastewater	332:341	arg1	removal					284:290	the effective removal	270:290	the effective removal of scavenging metal ions from water and wastewater	270:341	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	5	7	theme	energy	908:913	arg1	∆G°					923:925	∆G°	923:925	∆G°	923:925	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	7	theme	energy	908:913	arg1	change					915:920	Gibb's free energy change	896:920	Gibb's free energy change (∆G°)	896:926	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	1	8	theme	multi-functional	171:186	arg1	important					200:208	important	200:208	important	200:208	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	1	8	theme	multi-functional	171:186	arg1	chitosan					188:195	The multi-functional chitosan	167:195	The multi-functional chitosan	167:195	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	5	9	theme	Pb	1033:1034	arg1	ions					1075:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	6	10	from	mixture	1242:1248	arg1	regeneration					1180:1191	Batch regeneration	1174:1191	Batch regeneration of the spent adsorbent from the single component mixture	1174:1248	Batch regeneration of the spent adsorbent from the single component mixture was effective.					
30447369	4	11	theme	beads	770:774	arg1	performance					725:735	adsorption performance	714:735	adsorption performance of grafted cross-linked chitosan beads	714:774	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	4	12	theme	grafted	740:746	arg1	beads					770:774	grafted cross-linked chitosan beads	740:774	grafted cross-linked chitosan beads	740:774	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	1	13	theme	effective	274:282	arg1	removal					284:290	the effective removal	270:290	the effective removal of scavenging metal ions from water and wastewater	270:341	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	5	14	from	spontaneous	1135:1145	arg1	nature					1166:1171	nature	1166:1171	nature	1166:1171	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	1	15	from	water	322:326	arg1	removal					284:290	the effective removal	270:290	the effective removal of scavenging metal ions from water and wastewater	270:341	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	3	16	theme	batch	552:556	arg1	mode					558:561	batch mode	552:561	batch mode	552:561	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	7	17	theme	Multiple	1265:1272	arg1	experiments					1296:1306	Multiple adsorption/desorption experiments	1265:1306	Multiple adsorption/desorption experiments	1265:1306	Multiple adsorption/desorption experiments were also carried out, and it was found that 0.0% mass loss of the G/ECH-CS was observed after the third cycle of adsorption/desorption studies.					
30447369	6	18	theme	adsorbent	1206:1214	arg1	regeneration					1180:1191	Batch regeneration	1174:1191	Batch regeneration of the spent adsorbent from the single component mixture	1174:1248	Batch regeneration of the spent adsorbent from the single component mixture was effective.					
30447369	5	19	theme	Cu	1041:1042	arg1	ions					1075:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	7	20	theme	adsorption/desorption	1274:1294	arg1	experiments					1296:1306	Multiple adsorption/desorption experiments	1265:1306	Multiple adsorption/desorption experiments	1265:1306	Multiple adsorption/desorption experiments were also carried out, and it was found that 0.0% mass loss of the G/ECH-CS was observed after the third cycle of adsorption/desorption studies.					
30447369	5	21	theme	entropy	955:961	arg1	∆S°					971:973	∆S°	971:973	∆S°	971:973	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	21	theme	entropy	955:961	arg1	change					963:968	entropy change	955:968	entropy change (∆S°)	955:974	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	4	22	theme	kinetic	848:854	arg1	model					856:860	pseudo-second order kinetic model	828:860	pseudo-second order kinetic model	828:860	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	7	23	theme	third	1407:1411	arg1	cycle					1413:1417	the third cycle	1403:1417	the third cycle of adsorption/desorption studies	1403:1450	Multiple adsorption/desorption experiments were also carried out, and it was found that 0.0% mass loss of the G/ECH-CS was observed after the third cycle of adsorption/desorption studies.					
30447369	0	24	theme	heavy	9:13	arg1	ions					21:24	heavy metal ions	9:24	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid	0:58	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	6	25	theme	single	1225:1230	arg1	mixture					1242:1248	the single component mixture	1221:1248	the single component mixture	1221:1248	Batch regeneration of the spent adsorbent from the single component mixture was effective.					
30447369	5	26	link	crosslinked	1093:1103	arg1	G/ECH-CS					1121:1128	G/ECH-CS	1121:1128	G/ECH-CS	1121:1128	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	26	link	crosslinked	1093:1103	arg1	beads					1114:1118	grafted crosslinked chitosan beads	1085:1118	grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature	1085:1171	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	27	theme	enthalpy	929:936	arg1	∆H°					946:948	∆H°	946:948	∆H°	946:948	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	27	theme	enthalpy	929:936	arg1	change					938:943	enthalpy change	929:943	enthalpy change (∆H°)	929:949	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	0	28	theme	Enhanced	0:7	arg1	adsorption					26:35	Enhanced heavy metal ions adsorption	0:35	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid	0:58	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	7	29	theme	mass	1358:1361	arg1	loss					1363:1366	0.0% mass loss	1353:1366	0.0% mass loss of the G/ECH-CS	1353:1382	Multiple adsorption/desorption experiments were also carried out, and it was found that 0.0% mass loss of the G/ECH-CS was observed after the third cycle of adsorption/desorption studies.					
30447369	5	30	theme	free	903:906	arg1	∆G°					923:925	∆G°	923:925	∆G°	923:925	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	30	theme	free	903:906	arg1	change					915:920	Gibb's free energy change	896:920	Gibb's free energy change (∆G°)	896:926	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	0	31	theme	ions	21:24	arg1	adsorption					26:35	Enhanced heavy metal ions adsorption	0:35	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid	0:58	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	1	32	theme	scavenging	295:304	arg1	ions					312:315	scavenging metal ions	295:315	scavenging metal ions	295:315	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	5	33	dep	beads	1114:1118	arg1	endothermic					1151:1161	endothermic	1151:1161	endothermic	1151:1161	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	33	dep	beads	1114:1118	arg1	spontaneous					1135:1145	spontaneous	1135:1145	spontaneous	1135:1145	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	4	34	theme	cross-linked	748:759	arg1	beads					770:774	grafted cross-linked chitosan beads	740:774	grafted cross-linked chitosan beads	740:774	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	7	35	theme	studies	1444:1450	arg1	cycle					1413:1417	the third cycle	1403:1417	the third cycle of adsorption/desorption studies	1403:1450	Multiple adsorption/desorption experiments were also carried out, and it was found that 0.0% mass loss of the G/ECH-CS was observed after the third cycle of adsorption/desorption studies.					
30447369	0	36	theme	metal	15:19	arg1	ions					21:24	heavy metal ions	9:24	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid	0:58	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	1	37	theme	metal	306:310	arg1	ions					312:315	scavenging metal ions	295:315	scavenging metal ions	295:315	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	3	38	theme	maximum	634:640	arg1	removal					642:648	the maximum removal	630:648	the maximum removal of metal ions using batch applications	630:687	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	0	39	dep	grafted	60:66	arg1	Kinetics					107:114	Kinetics	107:114	Kinetics	107:114	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	0	39	dep	grafted	60:66	arg1	thermodynamics					128:141	thermodynamics	128:141	thermodynamics	128:141	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	0	39	dep	grafted	60:66	arg1	studies					158:164	desorption studies	147:164	desorption studies	147:164	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	0	39	dep	grafted	60:66	arg1	isotherms					117:125	isotherms	117:125	isotherms	117:125	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	3	40	theme	ionic	500:504	arg1	strength					506:513	ionic strength	500:513	ionic strength	500:513	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	1	41	theme	ions	312:315	arg1	removal					284:290	the effective removal	270:290	the effective removal of scavenging metal ions from water and wastewater	270:341	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	0	42	theme	desorption	147:156	arg1	studies					158:164	desorption studies	147:164	desorption studies	147:164	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	5	43	from	nature	1166:1171	arg1	spontaneous					1135:1145	spontaneous	1135:1145	spontaneous	1135:1145	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	3	44	theme	Physicochemical	445:459	arg1	time					494:497	contact time	486:497	contact time	486:497	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	3	44	theme	Physicochemical	445:459	arg1	parameters					461:470	Physicochemical parameters	445:470	Physicochemical parameters including pH, contact time, ionic strength and temperature	445:529	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	3	44	theme	Physicochemical	445:459	arg1	strength					506:513	ionic strength	500:513	ionic strength	500:513	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	3	44	theme	Physicochemical	445:459	arg1	temperature					519:529	temperature	519:529	temperature	519:529	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	3	44	theme	Physicochemical	445:459	arg1	pH					482:483	pH	482:483	pH	482:483	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	6	45	theme	Batch	1174:1178	arg1	regeneration					1180:1191	Batch regeneration	1174:1191	Batch regeneration of the spent adsorbent from the single component mixture	1174:1248	Batch regeneration of the spent adsorbent from the single component mixture was effective.					
30447369	4	46	theme	chitosan	761:768	arg1	beads					770:774	grafted cross-linked chitosan beads	740:774	grafted cross-linked chitosan beads	740:774	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	5	47	theme	Thermodynamic	863:875	arg1	change					915:920	Gibb's free energy change	896:920	Gibb's free energy change (∆G°)	896:926	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	47	theme	Thermodynamic	863:875	arg1	change					938:943	enthalpy change	929:943	enthalpy change (∆H°)	929:949	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	47	theme	Thermodynamic	863:875	arg1	parameters					877:886	Thermodynamic parameters	863:886	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°)	863:974	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	47	theme	Thermodynamic	863:875	arg1	change					963:968	entropy change	955:968	entropy change (∆S°)	955:974	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	1	48	theme	novel	233:237	arg1	adsorbents					239:248	novel adsorbents	233:248	novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater	233:341	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	7	49	theme	G/ECH-CS	1375:1382	arg1	loss					1363:1366	0.0% mass loss	1353:1366	0.0% mass loss of the G/ECH-CS	1353:1382	Multiple adsorption/desorption experiments were also carried out, and it was found that 0.0% mass loss of the G/ECH-CS was observed after the third cycle of adsorption/desorption studies.					
30447369	4	50	theme	order	842:846	arg1	model					856:860	pseudo-second order kinetic model	828:860	pseudo-second order kinetic model	828:860	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	5	51	theme	Ni	1049:1050	arg1	ions					1075:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	0	52	theme	4‑aminobenzoic	40:53	arg1	acid					55:58	4‑aminobenzoic acid	40:58	4‑aminobenzoic acid	40:58	Enhanced heavy metal ions adsorption by 4‑aminobenzoic acid grafted on chitosan/epichlorohydrin composite: Kinetics, isotherms, thermodynamics and desorption studies.					
30447369	3	53	theme	ions	659:662	arg1	removal					642:648	the maximum removal	630:648	the maximum removal of metal ions using batch applications	630:687	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	4	54	theme	pseudo-second	828:840	arg1	model					856:860	pseudo-second order kinetic model	828:860	pseudo-second order kinetic model	828:860	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	5	55	theme	Zn	1057:1058	arg1	ions					1075:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions	1033:1078	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	3	56	theme	commercial	609:618	arg1	scale					620:624	a commercial scale	607:624	a commercial scale for the maximum removal of metal ions using batch applications	607:687	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	4	57	theme	adsorption	714:723	arg1	performance					725:735	adsorption performance	714:735	adsorption performance of grafted cross-linked chitosan beads	714:774	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	1	58	theme	adsorbents	239:248	arg1	development					218:228	the development	214:228	the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater	214:341	The multi-functional chitosan is important for the development of novel adsorbents which is needed for the effective removal of scavenging metal ions from water and wastewater.					
30447369	7	59	theme	%	1356:1356	arg1	loss					1363:1366	0.0% mass loss	1353:1366	0.0% mass loss of the G/ECH-CS	1353:1382	Multiple adsorption/desorption experiments were also carried out, and it was found that 0.0% mass loss of the G/ECH-CS was observed after the third cycle of adsorption/desorption studies.					
30447369	3	60	theme	contact	486:492	arg1	time					494:497	contact time	486:497	contact time	486:497	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	3	61	theme	metal	653:657	arg1	ions					659:662	metal ions	653:662	metal ions using batch applications	653:687	Physicochemical parameters including pH, contact time, ionic strength and temperature were investigated in batch mode to optimize the conditions to be applied on a commercial scale for the maximum removal of metal ions using batch applications.					
30447369	4	62	theme	Langmuir	806:813	arg1	isotherm					815:822	Langmuir isotherm	806:822	Langmuir isotherm	806:822	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	4	63	link	cross-linked	748:759	arg1	beads					770:774	grafted cross-linked chitosan beads	740:774	grafted cross-linked chitosan beads	740:774	The results showed that adsorption performance of grafted cross-linked chitosan beads can be modeled efficiently by Langmuir isotherm and pseudo-second order kinetic model.					
30447369	5	64	theme	grafted	1085:1091	arg1	G/ECH-CS					1121:1128	G/ECH-CS	1121:1128	G/ECH-CS	1121:1128	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	64	theme	grafted	1085:1091	arg1	beads					1114:1118	grafted crosslinked chitosan beads	1085:1118	grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature	1085:1171	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	7	65	theme	adsorption/desorption	1422:1442	arg1	studies					1444:1450	adsorption/desorption studies	1422:1450	adsorption/desorption studies	1422:1450	Multiple adsorption/desorption experiments were also carried out, and it was found that 0.0% mass loss of the G/ECH-CS was observed after the third cycle of adsorption/desorption studies.					
30447369	5	66	theme	ions	1075:1078	arg1	adsorption					1019:1028	the adsorption	1015:1028	the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature	1015:1171	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	67	theme	chitosan	1105:1112	arg1	G/ECH-CS					1121:1128	G/ECH-CS	1121:1128	G/ECH-CS	1121:1128	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	67	theme	chitosan	1105:1112	arg1	beads					1114:1118	grafted crosslinked chitosan beads	1085:1118	grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature	1085:1171	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	68	theme	crosslinked	1093:1103	arg1	G/ECH-CS					1121:1128	G/ECH-CS	1121:1128	G/ECH-CS	1121:1128	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
30447369	5	68	theme	crosslinked	1093:1103	arg1	beads					1114:1118	grafted crosslinked chitosan beads	1085:1118	grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature	1085:1171	Thermodynamic parameters such as Gibb's free energy change (∆G°), enthalpy change (∆H°) and entropy change (∆S°) were calculated and the results showed the adsorption of Pb(II), Cu(II), Ni(II), Zn(II) and Cd(II) ions onto grafted crosslinked chitosan beads (G/ECH-CS) are spontaneous and endothermic in nature.					
29641767	0	0	from	application	18:28	arg1	production					44:53	dextranase production	33:53	dextranase production from Pochonia chlamydosporia (VC4)	33:88	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	0	0	from	application	18:28	arg1	application					98:108	its application	94:108	its application on dextran removal from sugarcane juice	94:148	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	1	1	from	reduction	292:300	arg1	juice					315:319	sugarcane juice	305:319	sugarcane juice	305:319	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
29641767	6	2	theme	dextran	1046:1052	arg1	content					1054:1060	the dextran content	1042:1060	the dextran content of the sugarcane juice once treated for 12 hours	1042:1109	The dextranases produced by fungus P. chlamydosporia reduced by 75% the dextran content of the sugarcane juice once treated for 12 hours, when compared to the control treatment.					
29641767	4	3	from	effect	683:688	arg1	removal					697:703	the removal	693:703	the removal of dextran present in sugarcane juice	693:741	The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated.					
29641767	4	4	attach	removal	697:703	arg2	present					716:722	dextran present	708:722	dextran present	708:722	The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated.					
29641767	4	4	attach	removal	697:703	arg3	juice					737:741	sugarcane juice	727:741	sugarcane juice	727:741	The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated.					
29641767	2	5	from	medium	426:431	arg1	effects					326:332	The effects	322:332	The effects	322:332	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	2	5	from	medium	426:431	arg1	components					398:407	different components	388:407	different components from the culture medium	388:431	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	0	6	from	juice	144:148	arg1	removal					121:127	dextran removal	113:127	dextran removal from sugarcane juice	113:148	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	5	7	theme	significant	809:819	arg1	effect					821:826	significant effect	809:826	significant effect	809:826	It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5.					
29641767	0	8	from	production	44:53	arg1	removal					121:127	dextran removal	113:127	dextran removal from sugarcane juice	113:148	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	1	9	from	activity	272:279	arg1	reduction					292:300	dextran reduction	284:300	dextran reduction in sugarcane juice	284:319	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
29641767	0	10	theme	Statistical	0:10	arg1	tools					12:16	Statistical tools	0:16	Statistical tools	0:16	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	2	11	theme	dextranase	362:371	arg1	production					373:382	the P. chlamydosporia dextranase production	340:382	the P. chlamydosporia dextranase production	340:382	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	2	12	from	effects	326:332	arg1	medium					426:431	the culture medium	414:431	the culture medium	414:431	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	1	13	theme	study	167:171	arg1	aim					155:157	The aim	151:157	The aim of this study	151:171	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
29641767	2	14	theme	culture	418:424	arg1	medium					426:431	the culture medium	414:431	the culture medium	414:431	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	4	15	theme	present	716:722	arg1	removal					697:703	the removal	693:703	the removal of dextran present in sugarcane juice	693:741	The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated.					
29641767	2	16	theme	P.	344:345	arg1	production					373:382	the P. chlamydosporia dextranase production	340:382	the P. chlamydosporia dextranase production	340:382	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	6	17	theme	control	1133:1139	arg1	treatment					1141:1149	the control treatment	1129:1149	the control treatment	1129:1149	The dextranases produced by fungus P. chlamydosporia reduced by 75% the dextran content of the sugarcane juice once treated for 12 hours, when compared to the control treatment.					
29641767	5	18	theme	enzyme	921:926	arg1	production					928:937	higher enzyme production	914:937	higher enzyme production	914:937	It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5.					
29641767	2	19	theme	components	398:407	arg1	effects					326:332	The effects	322:332	The effects	322:332	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	5	20	theme	higher	914:919	arg1	production					928:937	higher enzyme production	914:937	higher enzyme production	914:937	It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5.					
29641767	0	21	theme	dextran	113:119	arg1	removal					121:127	dextran removal	113:127	dextran removal from sugarcane juice	113:148	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	3	22	theme	higher	633:638	arg1	production					640:649	higher production	633:649	higher production of these enzymes	633:666	The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes.					
29641767	4	23	theme	sugarcane	727:735	arg1	juice					737:741	sugarcane juice	727:741	sugarcane juice	727:741	The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated.					
29641767	2	24	theme	different	388:396	arg1	components					398:407	different components	388:407	different components from the culture medium	388:431	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	2	25	theme	Plackett-Burman	450:464	arg1	design					466:471	Plackett-Burman design	450:471	Plackett-Burman design	450:471	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	0	26	theme	sugarcane	134:142	arg1	juice					144:148	sugarcane juice	134:148	sugarcane juice	134:148	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	3	27	used	utilized	528:535	arg2	surface					516:522	The response surface	503:522	The response surface	503:522	The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes.					
29641767	0	28	theme	dextranase	33:42	arg1	production					44:53	dextranase production	33:53	dextranase production from Pochonia chlamydosporia (VC4)	33:88	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	1	29	theme	dextranase	193:202	arg1	production					204:213	the dextranase production	189:213	the dextranase production by fungus Pochonia chlamydosporia (VC4)	189:253	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
29641767	1	30	theme	dextran	284:290	arg1	reduction					292:300	dextran reduction	284:300	dextran reduction in sugarcane juice	284:319	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
29641767	0	31	from	chlamydosporia	69:82	arg1	production					44:53	dextranase production	33:53	dextranase production from Pochonia chlamydosporia (VC4)	33:88	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	0	31	from	chlamydosporia	69:82	arg1	application					98:108	its application	94:108	its application on dextran removal from sugarcane juice	94:148	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	3	32	theme	enzymes	660:666	arg1	production					640:649	higher production	633:649	higher production of these enzymes	633:666	The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes.					
29641767	6	33	theme	juice	1079:1083	arg1	content					1054:1060	the dextran content	1042:1060	the dextran content of the sugarcane juice once treated for 12 hours	1042:1109	The dextranases produced by fungus P. chlamydosporia reduced by 75% the dextran content of the sugarcane juice once treated for 12 hours, when compared to the control treatment.					
29641767	0	34	from	application	98:108	arg1	removal					121:127	dextran removal	113:127	dextran removal from sugarcane juice	113:148	Statistical tools application on dextranase production from Pochonia chlamydosporia (VC4) and its application on dextran removal from sugarcane juice.					
29641767	3	35	theme	dextranase	602:611	arg1	production					613:622	dextranase production	602:622	dextranase production	602:622	The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes.					
29641767	4	36	theme	dextran	708:714	arg1	present					716:722	dextran present	708:722	dextran present	708:722	The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated.					
29641767	5	37	theme	dextranase	842:851	arg1	production					853:862	dextranase production	842:862	dextranase production	842:862	It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5.					
29641767	2	38	dep	P.	344:345	arg1	chlamydosporia					347:360	chlamydosporia	347:360	chlamydosporia	347:360	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	4	39	from	removal	697:703	arg1	juice					737:741	sugarcane juice	727:741	sugarcane juice	727:741	The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated.					
29641767	6	40	theme	sugarcane	1069:1077	arg1	juice					1079:1083	the sugarcane juice	1065:1083	the sugarcane juice once treated for 12 hours	1065:1109	The dextranases produced by fungus P. chlamydosporia reduced by 75% the dextran content of the sugarcane juice once treated for 12 hours, when compared to the control treatment.					
29641767	2	41	theme	composite	485:493	arg1	design					495:500	central composite design	477:500	central composite design	477:500	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	5	42	dep	showed	802:807	arg1	p<0.05					829:834	p<0.05	829:834	p<0.05	829:834	It was observed that only NaNO3 and pH showed significant effect (p<0.05) over dextranase production and was determined that the levels which provided higher enzyme production were, respectively, 5 g/L and 5.5.					
29641767	4	43	theme	enzymatic	673:681	arg1	effect					683:688	The enzymatic effect	669:688	The enzymatic effect on the removal of dextran present in sugarcane juice	669:741	The enzymatic effect on the removal of dextran present in sugarcane juice was also evaluated.					
29641767	1	44	theme	fungus	218:223	arg1	VC4					250:252	VC4	250:252	VC4	250:252	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
29641767	1	44	theme	fungus	218:223	arg1	chlamydosporia					234:247	fungus Pochonia chlamydosporia	218:247	fungus Pochonia chlamydosporia (VC4)	218:253	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
29641767	2	45	theme	central	477:483	arg1	design					495:500	central composite design	477:500	central composite design	477:500	The effects, over the P. chlamydosporia dextranase production, of different components from the culture medium were analyzed by Plackett-Burman design and central composite design.					
29641767	1	46	theme	sugarcane	305:313	arg1	juice					315:319	sugarcane juice	305:319	sugarcane juice	305:319	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
29641767	3	47	theme	response	507:514	arg1	surface					516:522	The response surface	503:522	The response surface	503:522	The response surface was utilized to determine the levels that, among the variables that influence dextranase production, provide higher production of these enzymes.					
29641767	1	48	theme	Pochonia	225:232	arg1	VC4					250:252	VC4	250:252	VC4	250:252	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
29641767	1	48	theme	Pochonia	225:232	arg1	chlamydosporia					234:247	fungus Pochonia chlamydosporia	218:247	fungus Pochonia chlamydosporia (VC4)	218:253	The aim of this study was to optimize the dextranase production by fungus Pochonia chlamydosporia (VC4) and evaluate its activity in dextran reduction in sugarcane juice.					
30790703	9	0	theme	mathematical	1378:1389	arg1	modeling					1391:1398	mathematical modeling	1378:1398	mathematical modeling	1378:1398	Besides, mathematical modeling indicated that the experimental fit biexponential equation.					
30790703	11	1	theme	drug	1746:1749	arg1	carriers					1751:1758	promising technological drug carriers	1722:1758	promising technological drug carriers	1722:1758	Therefore, these formulations could be promising technological drug carriers for acyclovir and curcumin, which highlight the great offering a potential alternative treatment for viral herpes.					
30790703	1	2	dep	overcome	327:334	arg1	co-encapsulated					299:313	co-encapsulated	299:313	co-encapsulated	299:313	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	10	3	theme	BoVH-1	1541:1546	arg1	virus					1548:1552	BoVH-1 virus	1541:1552	BoVH-1 virus	1541:1552	Importantly, drugs microencapsulation promoted superior antiviral effect against BoVH-1 virus in comparison to their free form, which could be attributed to the improvement in the aforementioned physicochemical parameters.					
30790703	8	4	theme	curcumin	1359:1366	arg1	solubility					1345:1354	the solubility	1341:1354	the solubility of curcumin	1341:1366	The in vitro release experiment demonstrated that the microencapsulation provided a sustained release of acyclovir as well as increased the solubility of curcumin.					
30790703	6	5	theme	unimodal	999:1006	arg1	distribution					1008:1019	unimodal distribution	999:1019	unimodal distribution	999:1019	The microparticles had spherical shape, size within 11.5-15.3 μm, unimodal distribution and no chemical interactions among the components of the formulations.					
30790703	0	6	theme	in	124:125	arg1	action					143:148	in vitro antiviral action	124:148	in vitro antiviral action	124:148	Co-encapsulation of acyclovir and curcumin into microparticles improves the physicochemical characteristics and potentiates in vitro antiviral action: Influence of the polymeric composition.					
30790703	1	7	theme	drugs	412:416	arg1	effect					397:402	the antiviral effect	383:402	the antiviral effect of both drugs	383:416	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	7	8	theme	polymeric	1122:1130	arg1	composition					1132:1142	the polymeric composition	1118:1142	the polymeric composition	1118:1142	Of particular importance, the polymeric composition considerably influenced on the release profile of the drugs.					
30790703	11	9	theme	viral	1861:1865	arg1	herpes					1867:1872	viral herpes	1861:1872	viral herpes	1861:1872	Therefore, these formulations could be promising technological drug carriers for acyclovir and curcumin, which highlight the great offering a potential alternative treatment for viral herpes.					
30790703	8	10	theme	in	1209:1210	arg1	experiment					1226:1235	The in vitro release experiment	1205:1235	The in vitro release experiment	1205:1235	The in vitro release experiment demonstrated that the microencapsulation provided a sustained release of acyclovir as well as increased the solubility of curcumin.					
30790703	0	11	theme	antiviral	133:141	arg1	action					143:148	in vitro antiviral action	124:148	in vitro antiviral action	124:148	Co-encapsulation of acyclovir and curcumin into microparticles improves the physicochemical characteristics and potentiates in vitro antiviral action: Influence of the polymeric composition.					
30790703	0	12	dep	in	124:125	arg1	vitro					127:131	vitro	127:131	vitro	127:131	Co-encapsulation of acyclovir and curcumin into microparticles improves the physicochemical characteristics and potentiates in vitro antiviral action: Influence of the polymeric composition.					
30790703	8	13	theme	sustained	1289:1297	arg1	release					1299:1305	a sustained release	1287:1305	a sustained release of acyclovir	1287:1318	The in vitro release experiment demonstrated that the microencapsulation provided a sustained release of acyclovir as well as increased the solubility of curcumin.					
30790703	2	14	theme	spray	457:461	arg1	methodology					470:480	a spray drying methodology	455:480	a spray drying methodology	455:480	The microparticles were prepared by a spray drying methodology following the ratio 1:3 (drug:polymer), which were made by hydroxypropylmethylcellulose (HPMC) and/or Eudragit® RS100 (EUD).					
30790703	3	15	theme	MP-3	711:714	arg1	formulation					716:726	MP-3 formulation	711:726	MP-3 formulation	711:726	The MP-1 formulation was composed of HPMC and EUD (1:1), MP-2 formulation was composed only of HPMC and MP-3 formulation was composed only of EUD.					
30790703	9	16	theme	biexponential	1436:1448	arg1	equation					1450:1457	the experimental fit biexponential equation	1415:1457	the experimental fit biexponential equation	1415:1457	Besides, mathematical modeling indicated that the experimental fit biexponential equation.					
30790703	1	17	theme	present	195:201	arg1	study					203:207	The present study	191:207	The present study developed and characterized	191:235	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	2	18	theme	drug	507:510	arg1	polymer					512:518	drug:polymer	507:518	drug:polymer	507:518	The microparticles were prepared by a spray drying methodology following the ratio 1:3 (drug:polymer), which were made by hydroxypropylmethylcellulose (HPMC) and/or Eudragit® RS100 (EUD).					
30790703	2	18	theme	drug	507:510	arg1	1:3					502:504	the ratio 1:3	492:504	the ratio 1:3 (drug:polymer)	492:519	The microparticles were prepared by a spray drying methodology following the ratio 1:3 (drug:polymer), which were made by hydroxypropylmethylcellulose (HPMC) and/or Eudragit® RS100 (EUD).					
30790703	7	19	theme	particular	1095:1104	arg1	importance					1106:1115	particular importance	1095:1115	particular importance	1095:1115	Of particular importance, the polymeric composition considerably influenced on the release profile of the drugs.					
30790703	10	20	theme	drugs	1473:1477	arg1	microencapsulation					1479:1496	drugs microencapsulation	1473:1496	drugs microencapsulation	1473:1496	Importantly, drugs microencapsulation promoted superior antiviral effect against BoVH-1 virus in comparison to their free form, which could be attributed to the improvement in the aforementioned physicochemical parameters.					
30790703	6	21	theme	formulations	1078:1089	arg1	components					1060:1069	the components	1056:1069	the components of the formulations	1056:1089	The microparticles had spherical shape, size within 11.5-15.3 μm, unimodal distribution and no chemical interactions among the components of the formulations.					
30790703	10	22	theme	antiviral	1516:1524	arg1	effect					1526:1531	superior antiviral effect	1507:1531	superior antiviral effect against BoVH-1 virus	1507:1552	Importantly, drugs microencapsulation promoted superior antiviral effect against BoVH-1 virus in comparison to their free form, which could be attributed to the improvement in the aforementioned physicochemical parameters.					
30790703	8	23	theme	release	1218:1224	arg1	experiment					1226:1235	The in vitro release experiment	1205:1235	The in vitro release experiment	1205:1235	The in vitro release experiment demonstrated that the microencapsulation provided a sustained release of acyclovir as well as increased the solubility of curcumin.					
30790703	10	24	theme	free	1577:1580	arg1	form					1582:1585	their free form	1571:1585	their free form	1571:1585	Importantly, drugs microencapsulation promoted superior antiviral effect against BoVH-1 virus in comparison to their free form, which could be attributed to the improvement in the aforementioned physicochemical parameters.					
30790703	4	25	theme	powder	813:818	arg1	flowability					820:830	acceptable powder flowability	802:830	acceptable powder flowability	802:830	All formulations showed yielding around 50% and acceptable powder flowability.					
30790703	5	26	theme	Drug	833:836	arg1	content					838:844	Drug content	833:844	Drug content	833:844	Drug content determination around 82.1-96.8% and 81.8-87% for acyclovir and curcumin, respectively.					
30790703	2	27	theme	ratio	496:500	arg1	polymer					512:518	drug:polymer	507:518	drug:polymer	507:518	The microparticles were prepared by a spray drying methodology following the ratio 1:3 (drug:polymer), which were made by hydroxypropylmethylcellulose (HPMC) and/or Eudragit® RS100 (EUD).					
30790703	2	27	theme	ratio	496:500	arg1	1:3					502:504	the ratio 1:3	492:504	the ratio 1:3 (drug:polymer)	492:519	The microparticles were prepared by a spray drying methodology following the ratio 1:3 (drug:polymer), which were made by hydroxypropylmethylcellulose (HPMC) and/or Eudragit® RS100 (EUD).					
30790703	1	28	theme	biopharmaceutical	340:356	arg1	limitations					358:368	the biopharmaceutical limitations	336:368	the biopharmaceutical limitations	336:368	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	7	29	theme	release	1175:1181	arg1	profile					1183:1189	the release profile	1171:1189	the release profile of the drugs	1171:1202	Of particular importance, the polymeric composition considerably influenced on the release profile of the drugs.					
30790703	10	30	theme	physicochemical	1655:1669	arg1	parameters					1671:1680	the aforementioned physicochemical parameters	1636:1680	the aforementioned physicochemical parameters	1636:1680	Importantly, drugs microencapsulation promoted superior antiviral effect against BoVH-1 virus in comparison to their free form, which could be attributed to the improvement in the aforementioned physicochemical parameters.					
30790703	1	31	dep	study	203:207	arg1	characterized					223:235	characterized	223:235	characterized	223:235	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	1	31	dep	study	203:207	arg1	developed					209:217	developed	209:217	developed	209:217	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	0	32	theme	curcumin	34:41	arg1	Co-encapsulation					0:15	Co-encapsulation	0:15	Co-encapsulation of acyclovir and curcumin into microparticles	0:61	Co-encapsulation of acyclovir and curcumin into microparticles improves the physicochemical characteristics and potentiates in vitro antiviral action: Influence of the polymeric composition.					
30790703	6	33	theme	spherical	956:964	arg1	shape					966:970	spherical shape	956:970	spherical shape	956:970	The microparticles had spherical shape, size within 11.5-15.3 μm, unimodal distribution and no chemical interactions among the components of the formulations.					
30790703	9	34	theme	experimental	1419:1430	arg1	equation					1450:1457	the experimental fit biexponential equation	1415:1457	the experimental fit biexponential equation	1415:1457	Besides, mathematical modeling indicated that the experimental fit biexponential equation.					
30790703	4	35	theme	acceptable	802:811	arg1	flowability					820:830	acceptable powder flowability	802:830	acceptable powder flowability	802:830	All formulations showed yielding around 50% and acceptable powder flowability.					
30790703	2	36	theme	drying	463:468	arg1	methodology					470:480	a spray drying methodology	455:480	a spray drying methodology	455:480	The microparticles were prepared by a spray drying methodology following the ratio 1:3 (drug:polymer), which were made by hydroxypropylmethylcellulose (HPMC) and/or Eudragit® RS100 (EUD).					
30790703	10	37	theme	aforementioned	1640:1653	arg1	parameters					1671:1680	the aforementioned physicochemical parameters	1636:1680	the aforementioned physicochemical parameters	1636:1680	Importantly, drugs microencapsulation promoted superior antiviral effect against BoVH-1 virus in comparison to their free form, which could be attributed to the improvement in the aforementioned physicochemical parameters.					
30790703	0	38	theme	polymeric	168:176	arg1	composition					178:188	the polymeric composition	164:188	the polymeric composition	164:188	Co-encapsulation of acyclovir and curcumin into microparticles improves the physicochemical characteristics and potentiates in vitro antiviral action: Influence of the polymeric composition.					
30790703	3	39	theme	MP-1	611:614	arg1	formulation					616:626	The MP-1 formulation	607:626	The MP-1 formulation	607:626	The MP-1 formulation was composed of HPMC and EUD (1:1), MP-2 formulation was composed only of HPMC and MP-3 formulation was composed only of EUD.					
30790703	9	40	dep	indicated	1400:1408	arg1	equation					1450:1457	the experimental fit biexponential equation	1415:1457	the experimental fit biexponential equation	1415:1457	Besides, mathematical modeling indicated that the experimental fit biexponential equation.					
30790703	11	41	theme	promising	1722:1730	arg1	carriers					1751:1758	promising technological drug carriers	1722:1758	promising technological drug carriers	1722:1758	Therefore, these formulations could be promising technological drug carriers for acyclovir and curcumin, which highlight the great offering a potential alternative treatment for viral herpes.					
30790703	1	42	contain	containing	265:274	arg2	curcumin					290:297	curcumin	290:297	curcumin	290:297	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	1	42	contain	containing	265:274	arg2	acyclovir					276:284	acyclovir	276:284	acyclovir	276:284	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	1	42	contain	containing	265:274	arg1	formulations					252:263	formulations	252:263	formulations containing acyclovir and curcumin	252:297	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	10	43	theme	superior	1507:1514	arg1	effect					1526:1531	superior antiviral effect	1507:1531	superior antiviral effect against BoVH-1 virus	1507:1552	Importantly, drugs microencapsulation promoted superior antiviral effect against BoVH-1 virus in comparison to their free form, which could be attributed to the improvement in the aforementioned physicochemical parameters.					
30790703	1	44	theme	antiviral	387:395	arg1	effect					397:402	the antiviral effect	383:402	the antiviral effect of both drugs	383:416	The present study developed and characterized microparticles formulations containing acyclovir and curcumin co-encapsulated in order to overcome the biopharmaceutical limitations and increase the antiviral effect of both drugs.					
30790703	8	45	dep	in	1209:1210	arg1	vitro					1212:1216	vitro	1212:1216	vitro	1212:1216	The in vitro release experiment demonstrated that the microencapsulation provided a sustained release of acyclovir as well as increased the solubility of curcumin.					
30790703	10	46	from	improvement	1621:1631	arg1	parameters					1671:1680	the aforementioned physicochemical parameters	1636:1680	the aforementioned physicochemical parameters	1636:1680	Importantly, drugs microencapsulation promoted superior antiviral effect against BoVH-1 virus in comparison to their free form, which could be attributed to the improvement in the aforementioned physicochemical parameters.					
30790703	9	47	theme	fit	1432:1434	arg1	equation					1450:1457	the experimental fit biexponential equation	1415:1457	the experimental fit biexponential equation	1415:1457	Besides, mathematical modeling indicated that the experimental fit biexponential equation.					
30790703	0	48	theme	composition	178:188	arg1	Influence					151:159	Influence	151:159	Influence of the polymeric composition	151:188	Co-encapsulation of acyclovir and curcumin into microparticles improves the physicochemical characteristics and potentiates in vitro antiviral action: Influence of the polymeric composition.					
30790703	3	49	theme	MP-2	664:667	arg1	formulation					669:679	MP-2 formulation	664:679	MP-2 formulation	664:679	The MP-1 formulation was composed of HPMC and EUD (1:1), MP-2 formulation was composed only of HPMC and MP-3 formulation was composed only of EUD.					
30790703	6	50	theme	chemical	1028:1035	arg1	interactions					1037:1048	no chemical interactions	1025:1048	no chemical interactions among the components of the formulations	1025:1089	The microparticles had spherical shape, size within 11.5-15.3 μm, unimodal distribution and no chemical interactions among the components of the formulations.					
30790703	0	51	theme	physicochemical	76:90	arg1	characteristics					92:106	the physicochemical characteristics	72:106	the physicochemical characteristics	72:106	Co-encapsulation of acyclovir and curcumin into microparticles improves the physicochemical characteristics and potentiates in vitro antiviral action: Influence of the polymeric composition.					
30790703	8	52	theme	acyclovir	1310:1318	arg1	release					1299:1305	a sustained release	1287:1305	a sustained release of acyclovir	1287:1318	The in vitro release experiment demonstrated that the microencapsulation provided a sustained release of acyclovir as well as increased the solubility of curcumin.					
30790703	6	53	contain	had	952:954	arg2	interactions					1037:1048	no chemical interactions	1025:1048	no chemical interactions among the components of the formulations	1025:1089	The microparticles had spherical shape, size within 11.5-15.3 μm, unimodal distribution and no chemical interactions among the components of the formulations.					
30790703	6	53	contain	had	952:954	arg2	size					973:976	size	973:976	size within 11.5-15.3 μm	973:996	The microparticles had spherical shape, size within 11.5-15.3 μm, unimodal distribution and no chemical interactions among the components of the formulations.					
30790703	6	53	contain	had	952:954	arg2	distribution					1008:1019	unimodal distribution	999:1019	unimodal distribution	999:1019	The microparticles had spherical shape, size within 11.5-15.3 μm, unimodal distribution and no chemical interactions among the components of the formulations.					
30790703	6	53	contain	had	952:954	arg1	microparticles					937:950	The microparticles	933:950	The microparticles	933:950	The microparticles had spherical shape, size within 11.5-15.3 μm, unimodal distribution and no chemical interactions among the components of the formulations.					
30790703	6	53	contain	had	952:954	arg2	shape					966:970	spherical shape	956:970	spherical shape	956:970	The microparticles had spherical shape, size within 11.5-15.3 μm, unimodal distribution and no chemical interactions among the components of the formulations.					
30790703	11	54	theme	potential	1825:1833	arg1	treatment					1847:1855	a potential alternative treatment	1823:1855	a potential alternative treatment for viral herpes	1823:1872	Therefore, these formulations could be promising technological drug carriers for acyclovir and curcumin, which highlight the great offering a potential alternative treatment for viral herpes.					
30790703	10	55	dep	form	1582:1585	arg1	comparison					1557:1566	comparison	1557:1566	comparison	1557:1566	Importantly, drugs microencapsulation promoted superior antiviral effect against BoVH-1 virus in comparison to their free form, which could be attributed to the improvement in the aforementioned physicochemical parameters.					
30790703	11	56	theme	technological	1732:1744	arg1	carriers					1751:1758	promising technological drug carriers	1722:1758	promising technological drug carriers	1722:1758	Therefore, these formulations could be promising technological drug carriers for acyclovir and curcumin, which highlight the great offering a potential alternative treatment for viral herpes.					
30790703	0	57	theme	acyclovir	20:28	arg1	Co-encapsulation					0:15	Co-encapsulation	0:15	Co-encapsulation of acyclovir and curcumin into microparticles	0:61	Co-encapsulation of acyclovir and curcumin into microparticles improves the physicochemical characteristics and potentiates in vitro antiviral action: Influence of the polymeric composition.					
30790703	7	58	theme	drugs	1198:1202	arg1	profile					1183:1189	the release profile	1171:1189	the release profile of the drugs	1171:1202	Of particular importance, the polymeric composition considerably influenced on the release profile of the drugs.					
30790703	11	59	theme	alternative	1835:1845	arg1	treatment					1847:1855	a potential alternative treatment	1823:1855	a potential alternative treatment for viral herpes	1823:1872	Therefore, these formulations could be promising technological drug carriers for acyclovir and curcumin, which highlight the great offering a potential alternative treatment for viral herpes.					
29427944	5	0	dep	either	848:853	arg1	cross-linked					866:877	cross-linked	866:877	covalently cross-linked using carbodiimide crosslinker chemistry	855:918	The CMBCD molecules were either covalently cross-linked using carbodiimide crosslinker chemistry or left unbound.					
29427944	11	1	theme	photoactivated	1757:1770	arg1	surfaces					1786:1793	photoactivated antimicrobial surfaces	1757:1793	photoactivated antimicrobial surfaces controlling bacterial contamination and spread	1757:1840	Stabilized cross-linked curcumin-loaded coatings can serve a basis for developing photoactivated antimicrobial surfaces controlling bacterial contamination and spread.					
29427944	3	2	theme	deposited	549:557	arg1	PLL					593:595	PLL	593:595	PLL	593:595	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	3	2	theme	deposited	549:557	arg1	poly-l-lysine					578:590	alternately deposited positively-charged poly-l-lysine	537:590	alternately deposited positively-charged poly-l-lysine (PLL)	537:596	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	6	3	theme	energy	1063:1068	arg1	spectroscopy					1087:1098	energy dispersive x-ray spectroscopy	1063:1098	energy dispersive x-ray spectroscopy	1063:1098	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	10	4	theme	photodynamic	1650:1661	arg1	properties					1663:1672	the photodynamic properties	1646:1672	the photodynamic properties	1646:1672	After the cross-linking, the coating lost its dark antimicrobial activity but retained the photodynamic properties.					
29427944	0	5	from	coatings	14:21	arg1	terephthalate					39:51	polyethylene terephthalate	26:51	polyethylene terephthalate	26:51	Antimicrobial coatings on polyethylene terephthalate based on curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture.					
29427944	11	6	link	cross-linked	1686:1697	arg1	coatings					1715:1722	Stabilized cross-linked curcumin-loaded coatings	1675:1722	Stabilized cross-linked curcumin-loaded coatings	1675:1722	Stabilized cross-linked curcumin-loaded coatings can serve a basis for developing photoactivated antimicrobial surfaces controlling bacterial contamination and spread.					
29427944	3	7	theme	positively-charged	559:576	arg1	PLL					593:595	PLL	593:595	PLL	593:595	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	3	7	theme	positively-charged	559:576	arg1	poly-l-lysine					578:590	alternately deposited positively-charged poly-l-lysine	537:590	alternately deposited positively-charged poly-l-lysine (PLL)	537:596	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	7	8	theme	compound	1193:1200	arg1	curcumin					1202:1209	a natural phenolic compound curcumin	1174:1209	a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin	1174:1257	To impart antimicrobial properties to the coatings they were loaded with a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin.					
29427944	0	9	theme	polyelectrolyte	117:131	arg1	architecture					133:144	a multilayer polyelectrolyte architecture	104:144	a multilayer polyelectrolyte architecture	104:144	Antimicrobial coatings on polyethylene terephthalate based on curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture.					
29427944	11	10	theme	bacterial	1807:1815	arg1	contamination					1817:1829	contamination	1817:1829	contamination	1817:1829	Stabilized cross-linked curcumin-loaded coatings can serve a basis for developing photoactivated antimicrobial surfaces controlling bacterial contamination and spread.					
29427944	7	11	theme	natural	1176:1182	arg1	curcumin					1202:1209	a natural phenolic compound curcumin	1174:1209	a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin	1174:1257	To impart antimicrobial properties to the coatings they were loaded with a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin.					
29427944	11	12	theme	Stabilized	1675:1684	arg1	coatings					1715:1722	Stabilized cross-linked curcumin-loaded coatings	1675:1722	Stabilized cross-linked curcumin-loaded coatings	1675:1722	Stabilized cross-linked curcumin-loaded coatings can serve a basis for developing photoactivated antimicrobial surfaces controlling bacterial contamination and spread.					
29427944	2	13	theme	terephthalate	336:348	arg1	matrix					374:379	a support matrix	364:379	a support matrix	364:379	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	2	13	theme	terephthalate	336:348	arg1	film					356:359	polyethylene terephthalate (PET) film	323:359	polyethylene terephthalate (PET) film	323:359	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	9	14	theme	cyclodextrin	1527:1538	arg1	complex					1550:1556	cyclodextrin inclusion complex	1527:1556	cyclodextrin inclusion complex	1527:1556	Curcumin was released from the non-cross-linked coatings into an aqueous medium in the form of cyclodextrin inclusion complex.					
29427944	0	15	theme	multilayer	106:115	arg1	architecture					133:144	a multilayer polyelectrolyte architecture	104:144	a multilayer polyelectrolyte architecture	104:144	Antimicrobial coatings on polyethylene terephthalate based on curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture.					
29427944	3	16	theme	negatively-charged	602:619	arg1	acid					637:640	negatively-charged poly-l-glutamic acid	602:640	negatively-charged poly-l-glutamic acid (PLGA)	602:647	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	3	16	theme	negatively-charged	602:619	arg1	CMBCD					683:687	CMBCD	683:687	CMBCD	683:687	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	3	16	theme	negatively-charged	602:619	arg1	PLGA					643:646	PLGA	643:646	PLGA	643:646	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	2	17	theme	polyethylene	323:334	arg1	matrix					374:379	a support matrix	364:379	a support matrix	364:379	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	2	17	theme	polyethylene	323:334	arg1	film					356:359	polyethylene terephthalate (PET) film	323:359	polyethylene terephthalate (PET) film	323:359	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	6	18	theme	electron	1039:1046	arg1	microscopy					1048:1057	scanning electron microscopy	1030:1057	scanning electron microscopy	1030:1057	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	8	19	theme	non-cross-linked	1264:1279	arg1	coatings					1281:1288	The non-cross-linked coatings	1260:1288	The non-cross-linked coatings	1260:1288	The non-cross-linked coatings showed bactericidal activity towards Escherichia coli in the dark, and this activity was further enhanced upon illumination with white light.					
29427944	6	20	theme	surface	941:947	arg1	morphology					949:958	surface morphology	941:958	surface morphology	941:958	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	7	21	with	complexes	1229:1237	arg1	β-cyclodextrin					1244:1257	β-cyclodextrin	1244:1257	β-cyclodextrin	1244:1257	To impart antimicrobial properties to the coatings they were loaded with a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin.					
29427944	9	22	theme	complex	1550:1556	arg1	form					1519:1522	the form	1515:1522	the form of cyclodextrin inclusion complex	1515:1556	Curcumin was released from the non-cross-linked coatings into an aqueous medium in the form of cyclodextrin inclusion complex.					
29427944	9	23	theme	inclusion	1540:1548	arg1	complex					1550:1556	cyclodextrin inclusion complex	1527:1556	cyclodextrin inclusion complex	1527:1556	Curcumin was released from the non-cross-linked coatings into an aqueous medium in the form of cyclodextrin inclusion complex.					
29427944	3	24	theme	sufficient	421:430	arg1	charge					441:446	sufficient electric charge	421:446	sufficient electric charge to the PET surface	421:465	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	4	25	theme	repeated	778:785	arg1	multilayers					787:797	repeated multilayers n varying from 5 to 20	778:820	repeated multilayers n varying from 5 to 20	778:820	The coatings had an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n, with the number of repeated multilayers n varying from 5 to 20.					
29427944	3	26	theme	PET	455:457	arg1	surface					459:465	the PET surface	451:465	the PET surface	451:465	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	0	27	theme	Antimicrobial	0:12	arg1	coatings					14:21	Antimicrobial coatings	0:21	Antimicrobial coatings on polyethylene terephthalate	0:51	Antimicrobial coatings on polyethylene terephthalate based on curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture.					
29427944	6	28	theme	scanning	1030:1037	arg1	microscopy					1048:1057	scanning electron microscopy	1030:1057	scanning electron microscopy	1030:1057	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	10	29	theme	antimicrobial	1610:1622	arg1	activity					1624:1631	its dark antimicrobial activity	1601:1631	its dark antimicrobial activity	1601:1631	After the cross-linking, the coating lost its dark antimicrobial activity but retained the photodynamic properties.					
29427944	11	30	theme	cross-linked	1686:1697	arg1	coatings					1715:1722	Stabilized cross-linked curcumin-loaded coatings	1675:1722	Stabilized cross-linked curcumin-loaded coatings	1675:1722	Stabilized cross-linked curcumin-loaded coatings can serve a basis for developing photoactivated antimicrobial surfaces controlling bacterial contamination and spread.					
29427944	4	31	dep	20	819:820	arg1	to					816:817	to	816:817	to	816:817	The coatings had an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n, with the number of repeated multilayers n varying from 5 to 20.					
29427944	0	32	theme	polyethylene	26:37	arg1	terephthalate					39:51	polyethylene terephthalate	26:51	polyethylene terephthalate	26:51	Antimicrobial coatings on polyethylene terephthalate based on curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture.					
29427944	7	33	theme	phenolic	1184:1191	arg1	curcumin					1202:1209	a natural phenolic compound curcumin	1174:1209	a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin	1174:1257	To impart antimicrobial properties to the coatings they were loaded with a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin.					
29427944	11	34	theme	curcumin-loaded	1699:1713	arg1	coatings					1715:1722	Stabilized cross-linked curcumin-loaded coatings	1675:1722	Stabilized cross-linked curcumin-loaded coatings	1675:1722	Stabilized cross-linked curcumin-loaded coatings can serve a basis for developing photoactivated antimicrobial surfaces controlling bacterial contamination and spread.					
29427944	2	35	theme	PET	351:353	arg1	matrix					374:379	a support matrix	364:379	a support matrix	364:379	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	2	35	theme	PET	351:353	arg1	film					356:359	polyethylene terephthalate (PET) film	323:359	polyethylene terephthalate (PET) film	323:359	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	8	36	theme	bactericidal	1297:1308	arg1	activity					1310:1317	bactericidal activity	1297:1317	bactericidal activity towards Escherichia coli in the dark	1297:1354	The non-cross-linked coatings showed bactericidal activity towards Escherichia coli in the dark, and this activity was further enhanced upon illumination with white light.					
29427944	10	37	theme	dark	1605:1608	arg1	activity					1624:1631	its dark antimicrobial activity	1601:1631	its dark antimicrobial activity	1601:1631	After the cross-linking, the coating lost its dark antimicrobial activity but retained the photodynamic properties.					
29427944	4	38	theme	multilayers	787:797	arg1	number					768:773	the number	764:773	the number of repeated multilayers n varying from 5 to 20	764:820	The coatings had an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n, with the number of repeated multilayers n varying from 5 to 20.					
29427944	4	39	dep	multilayers	787:797	arg1	varying					801:807	varying	801:807	varying from 5 to 20	801:820	The coatings had an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n, with the number of repeated multilayers n varying from 5 to 20.					
29427944	8	40	with	illumination	1401:1412	arg1	light					1425:1429	white light	1419:1429	white light	1419:1429	The non-cross-linked coatings showed bactericidal activity towards Escherichia coli in the dark, and this activity was further enhanced upon illumination with white light.					
29427944	4	41	dep	architecture	711:722	arg1	n					756:756	6-(PLL-PLGA-PLL-CMBCD)n	734:756	an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n	708:756	The coatings had an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n, with the number of repeated multilayers n varying from 5 to 20.					
29427944	5	42	theme	carbodiimide	885:896	arg1	chemistry					910:918	carbodiimide crosslinker chemistry	885:918	carbodiimide crosslinker chemistry	885:918	The CMBCD molecules were either covalently cross-linked using carbodiimide crosslinker chemistry or left unbound.					
29427944	7	43	dep	loaded	1162:1167	arg1	impart					1104:1109	impart	1104:1109	To impart antimicrobial properties to the coatings	1101:1150	To impart antimicrobial properties to the coatings they were loaded with a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin.					
29427944	6	44	theme	coatings	1004:1011	arg1	structure					961:969	structure	961:969	structure	961:969	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	6	44	theme	coatings	1004:1011	arg1	composition					985:995	elemental composition	975:995	elemental composition	975:995	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	6	44	theme	coatings	1004:1011	arg1	morphology					949:958	surface morphology	941:958	surface morphology	941:958	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	0	45	from	embedded	92:99	arg1	architecture					133:144	a multilayer polyelectrolyte architecture	104:144	a multilayer polyelectrolyte architecture	104:144	Antimicrobial coatings on polyethylene terephthalate based on curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture.					
29427944	6	46	dep	morphology	949:958	arg1	The					937:939	The	937:939	The	937:939	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	9	47	attach	released	1445:1452	arg2	Curcumin					1432:1439	Curcumin	1432:1439	Curcumin	1432:1439	Curcumin was released from the non-cross-linked coatings into an aqueous medium in the form of cyclodextrin inclusion complex.					
29427944	9	47	attach	released	1445:1452	arg1	coatings					1480:1487	the non-cross-linked coatings	1459:1487	the non-cross-linked coatings	1459:1487	Curcumin was released from the non-cross-linked coatings into an aqueous medium in the form of cyclodextrin inclusion complex.					
29427944	5	48	theme	crosslinker	898:908	arg1	chemistry					910:918	carbodiimide crosslinker chemistry	885:918	carbodiimide crosslinker chemistry	885:918	The CMBCD molecules were either covalently cross-linked using carbodiimide crosslinker chemistry or left unbound.					
29427944	0	49	theme	curcumin/cyclodextrin	62:82	arg1	complex					84:90	curcumin/cyclodextrin complex	62:90	curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture	62:144	Antimicrobial coatings on polyethylene terephthalate based on curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture.					
29427944	9	50	theme	non-cross-linked	1463:1478	arg1	coatings					1480:1487	the non-cross-linked coatings	1459:1487	the non-cross-linked coatings	1459:1487	Curcumin was released from the non-cross-linked coatings into an aqueous medium in the form of cyclodextrin inclusion complex.					
29427944	7	51	theme	antimicrobial	1111:1123	arg1	properties					1125:1134	antimicrobial properties	1111:1134	antimicrobial properties	1111:1134	To impart antimicrobial properties to the coatings they were loaded with a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin.					
29427944	4	52	contain	had	704:706	arg1	coatings					695:702	The coatings	691:702	The coatings	691:702	The coatings had an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n, with the number of repeated multilayers n varying from 5 to 20.					
29427944	4	52	contain	had	704:706	arg2	architecture					711:722	an architecture	708:722	an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n	708:756	The coatings had an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n, with the number of repeated multilayers n varying from 5 to 20.					
29427944	4	52	contain	had	704:706	arg2	PLL-PLGA					725:732	PLL-PLGA	725:732	PLL-PLGA	725:732	The coatings had an architecture (PLL-PLGA)6-(PLL-PLGA-PLL-CMBCD)n, with the number of repeated multilayers n varying from 5 to 20.					
29427944	2	53	theme	antimicrobial	242:254	arg1	coating					256:262	an antimicrobial coating	239:262	an antimicrobial coating based on curcumin-cyclodextrin inclusion complex	239:311	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	1	54	theme	Bacterial	147:155	arg1	worldwide					192:200	a growing concern worldwide	174:200	a growing concern worldwide	174:200	Bacterial contamination is a growing concern worldwide.					
29427944	1	54	theme	Bacterial	147:155	arg1	contamination					157:169	Bacterial contamination	147:169	Bacterial contamination	147:169	Bacterial contamination is a growing concern worldwide.					
29427944	7	55	theme	inclusion	1219:1227	arg1	complexes					1229:1237	inclusion complexes	1219:1237	inclusion complexes with β-cyclodextrin	1219:1257	To impart antimicrobial properties to the coatings they were loaded with a natural phenolic compound curcumin forming inclusion complexes with β-cyclodextrin.					
29427944	8	56	from	activity	1310:1317	arg1	dark					1351:1354	the dark	1347:1354	the dark	1347:1354	The non-cross-linked coatings showed bactericidal activity towards Escherichia coli in the dark, and this activity was further enhanced upon illumination with white light.					
29427944	9	57	link	non-cross-linked	1463:1478	arg1	coatings					1480:1487	the non-cross-linked coatings	1459:1487	the non-cross-linked coatings	1459:1487	Curcumin was released from the non-cross-linked coatings into an aqueous medium in the form of cyclodextrin inclusion complex.					
29427944	2	58	theme	support	366:372	arg1	matrix					374:379	a support matrix	364:379	a support matrix	364:379	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	2	58	theme	support	366:372	arg1	film					356:359	polyethylene terephthalate (PET) film	323:359	polyethylene terephthalate (PET) film	323:359	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	0	59	from	architecture	133:144	arg1	embedded					92:99	embedded	92:99	embedded	92:99	Antimicrobial coatings on polyethylene terephthalate based on curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture.					
29427944	3	60	theme	repeated	505:512	arg1	multilayers					514:524	repeated multilayers	505:524	repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD)	505:688	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	3	61	theme	poly-l-glutamic	621:635	arg1	acid					637:640	negatively-charged poly-l-glutamic acid	602:640	negatively-charged poly-l-glutamic acid (PLGA)	602:647	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	3	61	theme	poly-l-glutamic	621:635	arg1	CMBCD					683:687	CMBCD	683:687	CMBCD	683:687	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	3	61	theme	poly-l-glutamic	621:635	arg1	PLGA					643:646	PLGA	643:646	PLGA	643:646	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	9	62	theme	aqueous	1497:1503	arg1	medium					1505:1510	an aqueous medium	1494:1510	an aqueous medium in the form of cyclodextrin inclusion complex	1494:1556	Curcumin was released from the non-cross-linked coatings into an aqueous medium in the form of cyclodextrin inclusion complex.					
29427944	9	63	from	medium	1505:1510	arg1	form					1519:1522	the form	1515:1522	the form of cyclodextrin inclusion complex	1515:1556	Curcumin was released from the non-cross-linked coatings into an aqueous medium in the form of cyclodextrin inclusion complex.					
29427944	2	64	theme	inclusion	295:303	arg1	complex					305:311	curcumin-cyclodextrin inclusion complex	273:311	curcumin-cyclodextrin inclusion complex	273:311	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	8	65	link	non-cross-linked	1264:1279	arg1	coatings					1281:1288	The non-cross-linked coatings	1260:1288	The non-cross-linked coatings	1260:1288	The non-cross-linked coatings showed bactericidal activity towards Escherichia coli in the dark, and this activity was further enhanced upon illumination with white light.					
29427944	8	66	theme	white	1419:1423	arg1	light					1425:1429	white light	1419:1429	white light	1419:1429	The non-cross-linked coatings showed bactericidal activity towards Escherichia coli in the dark, and this activity was further enhanced upon illumination with white light.					
29427944	0	67	theme	embedded	92:99	arg1	complex					84:90	curcumin/cyclodextrin complex	62:90	curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture	62:144	Antimicrobial coatings on polyethylene terephthalate based on curcumin/cyclodextrin complex embedded in a multilayer polyelectrolyte architecture.					
29427944	2	68	theme	work	219:222	arg1	aim					207:209	The aim	203:209	The aim of this work	203:222	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	2	69	theme	curcumin-cyclodextrin	273:293	arg1	complex					305:311	curcumin-cyclodextrin inclusion complex	273:311	curcumin-cyclodextrin inclusion complex	273:311	The aim of this work was to develop an antimicrobial coating based on curcumin-cyclodextrin inclusion complex and using polyethylene terephthalate (PET) film as a support matrix.					
29427944	11	70	theme	antimicrobial	1772:1784	arg1	surfaces					1786:1793	photoactivated antimicrobial surfaces	1757:1793	photoactivated antimicrobial surfaces controlling bacterial contamination and spread	1757:1840	Stabilized cross-linked curcumin-loaded coatings can serve a basis for developing photoactivated antimicrobial surfaces controlling bacterial contamination and spread.					
29427944	1	71	theme	growing	176:182	arg1	worldwide					192:200	a growing concern worldwide	174:200	a growing concern worldwide	174:200	Bacterial contamination is a growing concern worldwide.					
29427944	1	71	theme	growing	176:182	arg1	contamination					157:169	Bacterial contamination	147:169	Bacterial contamination	147:169	Bacterial contamination is a growing concern worldwide.					
29427944	6	72	theme	x-ray	1081:1085	arg1	spectroscopy					1087:1098	energy dispersive x-ray spectroscopy	1063:1098	energy dispersive x-ray spectroscopy	1063:1098	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	3	73	theme	electric	432:439	arg1	charge					441:446	sufficient electric charge	421:446	sufficient electric charge to the PET surface	421:465	After a pre-treatment aimed to provide sufficient electric charge to the PET surface, it was electrostatically coated with repeated multilayers comprising alternately deposited positively-charged poly-l-lysine (PLL) and negatively-charged poly-l-glutamic acid (PLGA) and carboxymethyl-β-cyclodextrin (CMBCD).					
29427944	6	74	theme	elemental	975:983	arg1	composition					985:995	elemental composition	975:995	elemental composition	975:995	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29427944	1	75	theme	concern	184:190	arg1	worldwide					192:200	a growing concern worldwide	174:200	a growing concern worldwide	174:200	Bacterial contamination is a growing concern worldwide.					
29427944	1	75	theme	concern	184:190	arg1	contamination					157:169	Bacterial contamination	147:169	Bacterial contamination	147:169	Bacterial contamination is a growing concern worldwide.					
29427944	6	76	theme	dispersive	1070:1079	arg1	spectroscopy					1087:1098	energy dispersive x-ray spectroscopy	1063:1098	energy dispersive x-ray spectroscopy	1063:1098	The surface morphology, structure and elemental composition of the coatings were analysed by scanning electron microscopy and energy dispersive x-ray spectroscopy.					
29444351	6	0	theme	1.0	1314:1316	arg1	%					1317:1317	%	1317:1317	%	1317:1317	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	5	1	theme	cholesterol	1188:1198	arg1	treatment					1153:1161	the 1.0% IMNO treatment	1139:1161	the 1.0% IMNO treatment	1139:1161	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	1	theme	cholesterol	1188:1198	arg1	levels					1178:1183	the lowest levels	1167:1183	the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment	1167:1243	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	2	2	dep	%	540:540	arg1	1.0					529:531	1.0	529:531	1.0	529:531	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	2	2	dep	%	540:540	arg1	1.5					537:539	1.5	537:539	1.5	537:539	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	2	3	theme	per	443:445	arg1	tank					447:450	10 fish per tank	435:450	10 fish per tank	435:450	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	2	3	theme	per	443:445	arg1	tanks					428:432	12 tanks	425:432	12 tanks (10 fish per tank)	425:451	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	1	4	theme	Siberian	312:319	arg1	baeri					340:344	Siberian Sturgeon Acipenser baeri	312:344	Siberian Sturgeon Acipenser baeri	312:344	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	0	5	theme	Biochemical	163:173	arg1	Parameters					175:184	Hematological and Serum Biochemical Parameters	139:184	Hematological and Serum Biochemical Parameters	139:184	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	6	6	theme	study	1267:1271	arg1	findings					1250:1257	The findings	1246:1257	The findings of this study	1246:1271	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	3	7	contain	containing	795:804	arg1	diets					789:793	the control and experimental diets	760:793	the control and experimental diets containing 0.5% and 1.5% IMNO	760:823	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	3	7	contain	containing	795:804	arg2	%					818:818	1.5%	815:818	1.5% IMNO	815:823	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	3	7	contain	containing	795:804	arg2	%					809:809	0.5%	806:809	0.5%	806:809	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	1	8	theme	Sturgeon	321:328	arg1	baeri					340:344	Siberian Sturgeon Acipenser baeri	312:344	Siberian Sturgeon Acipenser baeri	312:344	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	5	9	theme	Siberian	1032:1039	arg1	Sturgeon					1041:1048	Siberian Sturgeon	1032:1048	Siberian Sturgeon	1032:1048	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	3	10	theme	significant	714:724	arg1	differences					726:736	no significant differences	711:736	no significant differences	711:736	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	1	11	theme	Acipenser	330:338	arg1	baeri					340:344	Siberian Sturgeon Acipenser baeri	312:344	Siberian Sturgeon Acipenser baeri	312:344	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	6	12	from	diet	1327:1330	arg1	administration					1296:1309	the administration	1292:1309	the administration of 1.0% IMNO in diet	1292:1330	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	6	13	theme	Sturgeon	1420:1427	arg1	parameters					1397:1406	some serum biochemical parameters	1374:1406	some serum biochemical parameters of Siberian Sturgeon	1374:1427	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	6	13	theme	Sturgeon	1420:1427	arg1	factors					1362:1368	the growth factors	1351:1368	the growth factors	1351:1368	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	6	14	theme	%	1317:1317	arg1	IMNO					1319:1322	1.0% IMNO	1314:1322	1.0% IMNO in diet	1314:1330	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	5	15	theme	IMNO	1148:1151	arg1	treatment					1153:1161	the 1.0% IMNO treatment	1139:1161	the 1.0% IMNO treatment	1139:1161	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	1	16	theme	baeri	340:344	arg1	composition					297:307	carcass composition	289:307	carcass composition	289:307	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	1	16	theme	baeri	340:344	arg1	indices					250:256	the growth indices	239:256	the growth indices	239:256	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	1	16	theme	baeri	340:344	arg1	parameters					273:282	hematological parameters	259:282	hematological parameters	259:282	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	3	17	theme	control	764:770	arg1	diets					789:793	the control and experimental diets	760:793	the control and experimental diets containing 0.5% and 1.5% IMNO	760:823	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	0	18	theme	Growth	97:102	arg1	Performance					104:114	Growth Performance	97:114	Growth Performance	97:114	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	3	19	theme	final	587:591	arg1	length					593:598	final length	587:598	final length	587:598	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	5	20	theme	1.5	1225:1227	arg1	%					1228:1228	%	1228:1228	%	1228:1228	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	0	21	theme	Siberian	12:19	arg1	baeri					40:44	Subyearling Siberian Sturgeon Acipenser baeri	0:44	Subyearling Siberian Sturgeon Acipenser baeri	0:44	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	5	22	theme	%	1228:1228	arg1	treatment					1235:1243	the 1.5% IMNO treatment	1221:1243	the 1.5% IMNO treatment	1221:1243	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	6	23	from	IMNO	1319:1322	arg1	diet					1327:1330	diet	1327:1330	diet	1327:1330	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	3	24	theme	final	601:605	arg1	weight					607:612	final weight	601:612	final weight	601:612	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	0	25	theme	Subyearling	0:10	arg1	baeri					40:44	Subyearling Siberian Sturgeon Acipenser baeri	0:44	Subyearling Siberian Sturgeon Acipenser baeri	0:44	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	1	26	theme	growth	243:248	arg1	indices					250:256	the growth indices	239:256	the growth indices	239:256	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	5	27	theme	IMNO	1230:1233	arg1	treatment					1235:1243	the 1.5% IMNO treatment	1221:1243	the 1.5% IMNO treatment	1221:1243	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	3	28	dep	%	818:818	arg1	IMNO					820:823	IMNO	820:823	1.5% IMNO	815:823	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	5	29	from	levels	1178:1183	arg1	treatment					1235:1243	the 1.5% IMNO treatment	1221:1243	the 1.5% IMNO treatment	1221:1243	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	0	30	theme	Acipenser	30:38	arg1	baeri					40:44	Subyearling Siberian Sturgeon Acipenser baeri	0:44	Subyearling Siberian Sturgeon Acipenser baeri	0:44	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	3	31	theme	weight	619:624	arg1	gain					626:629	weight gain	619:629	weight gain	619:629	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	0	32	theme	Body	117:120	arg1	Composition					122:132	Body Composition	117:132	Body Composition	117:132	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	6	33	theme	Siberian	1411:1418	arg1	Sturgeon					1420:1427	Siberian Sturgeon	1411:1427	Siberian Sturgeon	1411:1427	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	4	34	theme	composition	938:948	arg1	terms					892:896	terms	892:896	terms of hematological parameters and carcass composition	892:948	In addition, there were no significant differences among diets in terms of hematological parameters and carcass composition.					
29444351	6	35	from	administration	1296:1309	arg1	diet					1327:1330	diet	1327:1330	diet	1327:1330	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	0	36	theme	Sturgeon	21:28	arg1	baeri					40:44	Subyearling Siberian Sturgeon Acipenser baeri	0:44	Subyearling Siberian Sturgeon Acipenser baeri	0:44	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	5	37	theme	Sturgeon	1041:1048	arg1	cholesterol					1059:1069	cholesterol	1059:1069	cholesterol	1059:1069	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	37	theme	Sturgeon	1041:1048	arg1	parameters					1018:1027	the serum biochemical parameters	996:1027	the serum biochemical parameters	996:1027	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	37	theme	Sturgeon	1041:1048	arg1	triglycerides					1072:1084	triglycerides	1072:1084	triglycerides	1072:1084	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	37	theme	Sturgeon	1041:1048	arg1	IgM					1091:1093	IgM	1091:1093	IgM	1091:1093	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	38	theme	IgM	1122:1124	arg1	level					1113:1117	the highest level	1101:1117	the highest level of IgM	1101:1124	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	39	theme	%	1146:1146	arg1	treatment					1153:1161	the 1.0% IMNO treatment	1139:1161	the 1.0% IMNO treatment	1139:1161	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	40	theme	1.0	1143:1145	arg1	%					1146:1146	%	1146:1146	%	1146:1146	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	2	41	theme	fish	438:441	arg1	tank					447:450	10 fish per tank	435:450	10 fish per tank	435:450	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	2	41	theme	fish	438:441	arg1	tanks					428:432	12 tanks	425:432	12 tanks (10 fish per tank)	425:451	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	1	42	theme	hematological	259:271	arg1	parameters					273:282	hematological parameters	259:282	hematological parameters	259:282	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	2	43	theme	control	486:492	arg1	diet					494:497	a control diet	484:497	a control diet (0%)	484:502	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	2	43	theme	control	486:492	arg1	%					501:501	0%	500:501	0%	500:501	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	3	44	theme	experimental	776:787	arg1	diets					789:793	the control and experimental diets	760:793	the control and experimental diets containing 0.5% and 1.5% IMNO	760:823	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	3	45	theme	1.0	693:695	arg1	%					696:696	%	696:696	%	696:696	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	4	46	theme	carcass	930:936	arg1	composition					938:948	carcass composition	930:948	carcass composition	930:948	In addition, there were no significant differences among diets in terms of hematological parameters and carcass composition.					
29444351	2	47	theme	triplicate	457:466	arg1	groups					468:473	triplicate groups	457:473	triplicate groups	457:473	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	5	48	theme	serum	1000:1004	arg1	cholesterol					1059:1069	cholesterol	1059:1069	cholesterol	1059:1069	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	48	theme	serum	1000:1004	arg1	parameters					1018:1027	the serum biochemical parameters	996:1027	the serum biochemical parameters	996:1027	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	48	theme	serum	1000:1004	arg1	triglycerides					1072:1084	triglycerides	1072:1084	triglycerides	1072:1084	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	48	theme	serum	1000:1004	arg1	IgM					1091:1093	IgM	1091:1093	IgM	1091:1093	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	0	49	theme	Hematological	139:151	arg1	Parameters					175:184	Hematological and Serum Biochemical Parameters	139:184	Hematological and Serum Biochemical Parameters	139:184	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	4	50	from	differences	865:875	arg1	terms					892:896	terms	892:896	terms of hematological parameters and carcass composition	892:948	In addition, there were no significant differences among diets in terms of hematological parameters and carcass composition.					
29444351	0	51	dep	Fed	46:48	arg1	Effects					86:92	Effects	86:92	Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters	86:184	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	5	52	theme	triglycerides	1204:1216	arg1	treatment					1153:1161	the 1.0% IMNO treatment	1139:1161	the 1.0% IMNO treatment	1139:1161	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	52	theme	triglycerides	1204:1216	arg1	levels					1178:1183	the lowest levels	1167:1183	the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment	1167:1243	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	53	theme	biochemical	1006:1016	arg1	cholesterol					1059:1069	cholesterol	1059:1069	cholesterol	1059:1069	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	53	theme	biochemical	1006:1016	arg1	parameters					1018:1027	the serum biochemical parameters	996:1027	the serum biochemical parameters	996:1027	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	53	theme	biochemical	1006:1016	arg1	triglycerides					1072:1084	triglycerides	1072:1084	triglycerides	1072:1084	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	53	theme	biochemical	1006:1016	arg1	IgM					1091:1093	IgM	1091:1093	IgM	1091:1093	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	5	54	theme	dietary	960:966	arg1	IMNO					968:971	dietary IMNO	960:971	dietary IMNO	960:971	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	3	55	contain	containing	682:691	arg1	diet					677:680	the diet	673:680	the diet containing 1.0% IMNO	673:701	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	3	55	contain	containing	682:691	arg2	IMNO					698:701	1.0% IMNO	693:701	1.0% IMNO	693:701	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	6	56	theme	biochemical	1385:1395	arg1	parameters					1397:1406	some serum biochemical parameters	1374:1406	some serum biochemical parameters of Siberian Sturgeon	1374:1427	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	4	57	theme	parameters	915:924	arg1	terms					892:896	terms	892:896	terms of hematological parameters and carcass composition	892:948	In addition, there were no significant differences among diets in terms of hematological parameters and carcass composition.					
29444351	1	58	theme	carcass	289:295	arg1	composition					297:307	carcass composition	289:307	carcass composition	289:307	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	1	59	from	effect	209:214	arg1	composition					297:307	carcass composition	289:307	carcass composition	289:307	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	1	59	from	effect	209:214	arg1	indices					250:256	the growth indices	239:256	the growth indices	239:256	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	1	59	from	effect	209:214	arg1	parameters					273:282	hematological parameters	259:282	hematological parameters	259:282	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	6	60	theme	serum	1379:1383	arg1	parameters					1397:1406	some serum biochemical parameters	1374:1406	some serum biochemical parameters of Siberian Sturgeon	1374:1427	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	2	61	contain	containing	513:522	arg1	diets					507:511	diets	507:511	diets containing 0.5, 1.0, or 1.5% IMNO for 75 d	507:554	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	2	61	contain	containing	513:522	arg2	IMNO					542:545	0.5, 1.0, or 1.5% IMNO	524:545	0.5, 1.0, or 1.5% IMNO for 75 d	524:554	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
29444351	5	62	theme	highest	1105:1111	arg1	level					1113:1117	the highest level	1101:1117	the highest level of IgM	1101:1124	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	6	63	theme	growth	1355:1360	arg1	factors					1362:1368	the growth factors	1351:1368	the growth factors	1351:1368	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	5	64	theme	lowest	1171:1176	arg1	levels					1178:1183	the lowest levels	1167:1183	the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment	1167:1243	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	6	65	theme	IMNO	1319:1322	arg1	administration					1296:1309	the administration	1292:1309	the administration of 1.0% IMNO in diet	1292:1330	The findings of this study thus indicate that the administration of 1.0% IMNO in diet positively affects the growth factors and some serum biochemical parameters of Siberian Sturgeon.					
29444351	3	66	theme	%	696:696	arg1	IMNO					698:701	1.0% IMNO	693:701	1.0% IMNO	693:701	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	0	67	from	Effects	86:92	arg1	Composition					122:132	Body Composition	117:132	Body Composition	117:132	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	0	67	from	Effects	86:92	arg1	Parameters					175:184	Hematological and Serum Biochemical Parameters	139:184	Hematological and Serum Biochemical Parameters	139:184	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	0	67	from	Effects	86:92	arg1	Performance					104:114	Growth Performance	97:114	Growth Performance	97:114	Subyearling Siberian Sturgeon Acipenser baeri Fed a Diet Supplemented with ImmunoGen: Effects on Growth Performance, Body Composition, and Hematological and Serum Biochemical Parameters.					
29444351	4	68	theme	hematological	901:913	arg1	parameters					915:924	hematological parameters	901:924	hematological parameters	901:924	In addition, there were no significant differences among diets in terms of hematological parameters and carcass composition.					
29444351	1	69	theme	ImmunoGen	219:227	arg1	effect					209:214	the effect	205:214	the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri	205:344	In this research, the effect of ImmunoGen (IMNO) on the growth indices, hematological parameters, and carcass composition of Siberian Sturgeon Acipenser baeri was investigated.					
29444351	3	70	theme	experiment	575:584	arg1	end					564:566	the end	560:566	the end of the experiment	560:584	At the end of the experiment, final length, final weight, and weight gain were significantly higher in the fish fed the diet containing 1.0% IMNO, though no significant differences were observed between the control and experimental diets containing 0.5% and 1.5% IMNO.					
29444351	5	71	from	treatment	1153:1161	arg1	treatment					1235:1243	the 1.5% IMNO treatment	1221:1243	the 1.5% IMNO treatment	1221:1243	However, dietary IMNO significantly affected the serum biochemical parameters of Siberian Sturgeon, such as cholesterol, triglycerides, and IgM, with the highest level of IgM occurring in the 1.0% IMNO treatment and the lowest levels of cholesterol and triglycerides in the 1.5% IMNO treatment.					
29444351	4	72	theme	significant	853:863	arg1	differences					865:875	no significant differences	850:875	no significant differences among diets in terms of hematological parameters and carcass composition	850:948	In addition, there were no significant differences among diets in terms of hematological parameters and carcass composition.					
29444351	2	73	dep	IMNO	542:545	arg1	%					540:540	%	540:540	%	540:540	After acclimation, fish (274.8 ± 16.2 g) were allocated into 12 tanks (10 fish per tank) and triplicate groups were fed a control diet (0%) or diets containing 0.5, 1.0, or 1.5% IMNO for 75 d.					
31254578	0	0	theme	hybrid	62:67	arg1	nanocomposites					69:82	the hybrid nanocomposites	58:82	the hybrid nanocomposites for high-efficiency removal of Congo Red	58:123	In situ growth of ZIF-8 nanoparticles on chitosan to form the hybrid nanocomposites for high-efficiency removal of Congo Red.					
31254578	2	1	theme	X-ray	411:415	arg1	XPS					445:447	XPS	445:447	XPS	445:447	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	1	theme	X-ray	411:415	arg1	spectroscopy					431:442	X-ray photoelectron spectroscopy	411:442	X-ray photoelectron spectroscopy (XPS)	411:448	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	1	theme	X-ray	411:415	arg1	microscope					353:362	scanning electron microscope	335:362	scanning electron microscope (SEM)	335:368	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	1	2	theme	imidazole	139:147	arg1	CS					184:185	CS	184:185	CS	184:185	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	2	theme	imidazole	139:147	arg1	chitosan					174:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan	126:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS)	126:212	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	7	3	theme	bonds	1088:1092	arg1	participation					1062:1074	the participation	1058:1074	the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects	1058:1167	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	1	4	theme	framework	149:157	arg1	CS					184:185	CS	184:185	CS	184:185	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	4	theme	framework	149:157	arg1	chitosan					174:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan	126:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS)	126:212	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	6	5	theme	kinetics	859:866	arg1	parameters					868:877	The kinetics parameters	855:877	The kinetics parameters	855:877	The kinetics parameters were in accord with pseudo-second-order equation, which implied that the adsorption rate was mainly controlled by the chemisorption mechanism.					
31254578	3	6	from	behavior	536:543	arg1	CR					582:583	CR	582:583	CR	582:583	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	3	6	from	behavior	536:543	arg1	Red					577:579	Red	577:579	Red	577:579	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	3	6	from	behavior	536:543	arg1	solution					597:604	aqueous solution	589:604	aqueous solution	589:604	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	4	7	theme	isotherm	654:661	arg1	data					663:666	The adsorption isotherm data	639:666	The adsorption isotherm data	639:666	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	2	8	dep	Fourier	451:457	arg1	transform					459:467	transform	459:467	transform IR spectroscopy (FTIR) and X-ray diffraction (XRD)	459:518	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	9	theme	X-ray	496:500	arg1	XRD					515:517	XRD	515:517	XRD	515:517	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	9	theme	X-ray	496:500	arg1	diffraction					502:512	X-ray diffraction	496:512	X-ray diffraction (XRD)	496:518	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	7	10	theme	π-π	1123:1125	arg1	conjugation					1127:1137	π-π conjugation	1123:1137	π-π conjugation	1123:1137	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	2	11	theme	ZIF-8	305:309	arg1	CS					311:312	ZIF-8@CS	305:312	ZIF-8@CS	305:312	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	5	12	theme	maximum	801:807	arg1	capacity					820:827	The maximum adsorption capacity	797:827	The maximum adsorption capacity of ZIF-8@CS	797:839	The maximum adsorption capacity of ZIF-8@CS was 922 mg/g.					
31254578	5	12	theme	maximum	801:807	arg1	922 mg/g					845:852	922 mg/g	845:852	922 mg/g	845:852	The maximum adsorption capacity of ZIF-8@CS was 922 mg/g.					
31254578	7	13	theme	conjugation	1127:1137	arg1	participation					1062:1074	the participation	1058:1074	the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects	1058:1167	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	6	14	theme	adsorption	952:961	arg1	rate					963:966	the adsorption rate	948:966	the adsorption rate	948:966	The kinetics parameters were in accord with pseudo-second-order equation, which implied that the adsorption rate was mainly controlled by the chemisorption mechanism.					
31254578	2	15	theme	scanning	335:342	arg1	microscope					353:362	scanning electron microscope	335:362	scanning electron microscope (SEM)	335:368	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	15	theme	scanning	335:342	arg1	Fourier					451:457	Fourier	451:457	Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD)	451:518	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	15	theme	scanning	335:342	arg1	spectroscopy					431:442	X-ray photoelectron spectroscopy	411:442	X-ray photoelectron spectroscopy (XPS)	411:448	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	15	theme	scanning	335:342	arg1	microscope					393:402	transmission electron microscope	371:402	transmission electron microscope (TEM)	371:408	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	15	theme	scanning	335:342	arg1	SEM					365:367	SEM	365:367	SEM	365:367	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	0	16	theme	high-efficiency	88:102	arg1	removal					104:110	high-efficiency removal	88:110	high-efficiency removal of Congo Red	88:123	In situ growth of ZIF-8 nanoparticles on chitosan to form the hybrid nanocomposites for high-efficiency removal of Congo Red.					
31254578	0	17	theme	In	0:1	arg1	growth					8:13	In situ growth	0:13	In situ growth of ZIF-8	0:22	In situ growth of ZIF-8 nanoparticles on chitosan to form the hybrid nanocomposites for high-efficiency removal of Congo Red.					
31254578	1	18	theme	ZIF-8	160:164	arg1	CS					184:185	CS	184:185	CS	184:185	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	18	theme	ZIF-8	160:164	arg1	chitosan					174:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan	126:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS)	126:212	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	2	19	theme	electron	384:391	arg1	microscope					353:362	scanning electron microscope	335:362	scanning electron microscope (SEM)	335:368	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	19	theme	electron	384:391	arg1	TEM					405:407	TEM	405:407	TEM	405:407	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	19	theme	electron	384:391	arg1	microscope					393:402	transmission electron microscope	371:402	transmission electron microscope (TEM)	371:408	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	3	20	theme	aqueous	589:595	arg1	solution					597:604	aqueous solution	589:604	aqueous solution	589:604	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	5	21	theme	adsorption	809:818	arg1	capacity					820:827	The maximum adsorption capacity	797:827	The maximum adsorption capacity of ZIF-8@CS	797:839	The maximum adsorption capacity of ZIF-8@CS was 922 mg/g.					
31254578	5	21	theme	adsorption	809:818	arg1	922 mg/g					845:852	922 mg/g	845:852	922 mg/g	845:852	The maximum adsorption capacity of ZIF-8@CS was 922 mg/g.					
31254578	7	22	theme	coordination	1148:1159	arg1	effects					1161:1167	zinc coordination effects	1143:1167	zinc coordination effects	1143:1167	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	6	23	theme	chemisorption	997:1009	arg1	mechanism					1011:1019	the chemisorption mechanism	993:1019	the chemisorption mechanism	993:1019	The kinetics parameters were in accord with pseudo-second-order equation, which implied that the adsorption rate was mainly controlled by the chemisorption mechanism.					
31254578	2	24	theme	transmission	371:382	arg1	microscope					353:362	scanning electron microscope	335:362	scanning electron microscope (SEM)	335:368	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	24	theme	transmission	371:382	arg1	TEM					405:407	TEM	405:407	TEM	405:407	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	24	theme	transmission	371:382	arg1	microscope					393:402	transmission electron microscope	371:402	transmission electron microscope (TEM)	371:408	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	4	25	theme	single-layer	717:728	arg1	adsorption					684:693	the adsorption	680:693	the adsorption of CR by ZIF-8@CS	680:711	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	4	25	theme	single-layer	717:728	arg1	adsorption					730:739	single-layer adsorption	717:739	single-layer adsorption	717:739	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	1	26	theme	dotted	167:172	arg1	CS					184:185	CS	184:185	CS	184:185	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	26	theme	dotted	167:172	arg1	chitosan					174:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan	126:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS)	126:212	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	7	27	theme	CR	1037:1038	arg1	removal					1026:1032	The removal	1022:1032	The removal of CR	1022:1038	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	1	28	theme	in-situ	234:240	arg1	method					249:254	in-situ growth method	234:254	in-situ growth method	234:254	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	29	theme	chitosan	174:181	arg1	nanocomposites					188:201	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites	126:201	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS)	126:212	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	29	theme	chitosan	174:181	arg1	CS					210:211	ZIF-8@CS	204:211	ZIF-8@CS	204:211	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	30	theme	growth	242:247	arg1	method					249:254	in-situ growth method	234:254	in-situ growth method	234:254	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	2	31	theme	IR	469:470	arg1	FTIR					486:489	FTIR	486:489	FTIR	486:489	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	31	theme	IR	469:470	arg1	spectroscopy					472:483	IR spectroscopy	469:483	IR spectroscopy (FTIR)	469:490	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	3	32	theme	ZIF-8	548:552	arg1	composites					557:566	ZIF-8@CS composites	548:566	ZIF-8@CS composites on Congo Red (CR)	548:584	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	5	33	theme	ZIF-8	832:836	arg1	CS					838:839	ZIF-8@CS	832:839	ZIF-8@CS	832:839	The maximum adsorption capacity of ZIF-8@CS was 922 mg/g.					
31254578	5	34	theme	CS	838:839	arg1	capacity					820:827	The maximum adsorption capacity	797:827	The maximum adsorption capacity of ZIF-8@CS	797:839	The maximum adsorption capacity of ZIF-8@CS was 922 mg/g.					
31254578	5	34	theme	CS	838:839	arg1	922 mg/g					845:852	922 mg/g	845:852	922 mg/g	845:852	The maximum adsorption capacity of ZIF-8@CS was 922 mg/g.					
31254578	3	35	from	Red	577:579	arg1	behavior					536:543	The adsorption behavior	521:543	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution	521:604	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	5	36	theme	@	837:837	arg1	CS					838:839	ZIF-8@CS	832:839	ZIF-8@CS	832:839	The maximum adsorption capacity of ZIF-8@CS was 922 mg/g.					
31254578	3	37	theme	@	553:553	arg1	composites					557:566	ZIF-8@CS composites	548:566	ZIF-8@CS composites on Congo Red (CR)	548:584	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	0	38	theme	ZIF-8	18:22	arg1	growth					8:13	In situ growth	0:13	In situ growth of ZIF-8	0:22	In situ growth of ZIF-8 nanoparticles on chitosan to form the hybrid nanocomposites for high-efficiency removal of Congo Red.					
31254578	0	39	theme	Red	121:123	arg1	removal					104:110	high-efficiency removal	88:110	high-efficiency removal of Congo Red	88:123	In situ growth of ZIF-8 nanoparticles on chitosan to form the hybrid nanocomposites for high-efficiency removal of Congo Red.					
31254578	4	40	theme	adsorption	643:652	arg1	data					663:666	The adsorption isotherm data	639:666	The adsorption isotherm data	639:666	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	4	41	theme	CR	698:699	arg1	adsorption					684:693	the adsorption	680:693	the adsorption of CR by ZIF-8@CS	680:711	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	4	41	theme	CR	698:699	arg1	adsorption					730:739	single-layer adsorption	717:739	single-layer adsorption	717:739	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	2	42	theme	electron	344:351	arg1	microscope					353:362	scanning electron microscope	335:362	scanning electron microscope (SEM)	335:368	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	42	theme	electron	344:351	arg1	Fourier					451:457	Fourier	451:457	Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD)	451:518	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	42	theme	electron	344:351	arg1	spectroscopy					431:442	X-ray photoelectron spectroscopy	411:442	X-ray photoelectron spectroscopy (XPS)	411:448	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	42	theme	electron	344:351	arg1	microscope					393:402	transmission electron microscope	371:402	transmission electron microscope (TEM)	371:408	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	42	theme	electron	344:351	arg1	SEM					365:367	SEM	365:367	SEM	365:367	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	6	43	theme	pseudo-second-order	899:917	arg1	equation					919:926	pseudo-second-order equation	899:926	pseudo-second-order equation	899:926	The kinetics parameters were in accord with pseudo-second-order equation, which implied that the adsorption rate was mainly controlled by the chemisorption mechanism.					
31254578	3	44	from	composites	557:566	arg1	CR					582:583	CR	582:583	CR	582:583	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	3	44	from	composites	557:566	arg1	Red					577:579	Red	577:579	Red	577:579	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	4	45	theme	@	709:709	arg1	CS					710:711	ZIF-8@CS	704:711	ZIF-8@CS	704:711	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	7	46	theme	effects	1161:1167	arg1	participation					1062:1074	the participation	1058:1074	the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects	1058:1167	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	4	47	theme	ZIF-8	704:708	arg1	CS					710:711	ZIF-8@CS	704:711	ZIF-8@CS	704:711	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	7	48	theme	hydrogen	1079:1086	arg1	bonds					1088:1092	hydrogen bonds	1079:1092	hydrogen bonds	1079:1092	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	2	49	theme	chemical	287:294	arg1	state					296:300	chemical state	287:300	chemical state	287:300	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	4	50	with	consistent	752:761	arg1	model					790:794	the Langmuir isotherm model	768:794	the Langmuir isotherm model	768:794	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	4	51	theme	isotherm	781:788	arg1	model					790:794	the Langmuir isotherm model	768:794	the Langmuir isotherm model	768:794	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	7	52	theme	zinc	1143:1146	arg1	effects					1161:1167	zinc coordination effects	1143:1167	zinc coordination effects	1143:1167	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	4	53	theme	Langmuir	772:779	arg1	model					790:794	the Langmuir isotherm model	768:794	the Langmuir isotherm model	768:794	The adsorption isotherm data showed that the adsorption of CR by ZIF-8@CS was single-layer adsorption, which was consistent with the Langmuir isotherm model.					
31254578	3	54	theme	CS	554:555	arg1	composites					557:566	ZIF-8@CS composites	548:566	ZIF-8@CS composites on Congo Red (CR)	548:584	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	3	55	theme	adsorption	525:534	arg1	behavior					536:543	The adsorption behavior	521:543	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution	521:604	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	2	56	theme	@	310:310	arg1	CS					311:312	ZIF-8@CS	305:312	ZIF-8@CS	305:312	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	7	57	theme	electrostatic	1095:1107	arg1	interactions					1109:1120	electrostatic interactions	1095:1120	electrostatic interactions	1095:1120	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	3	58	theme	composites	557:566	arg1	behavior					536:543	The adsorption behavior	521:543	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution	521:604	The adsorption behavior of ZIF-8@CS composites on Congo Red (CR) in aqueous solution was systematically investigated.					
31254578	2	59	theme	CS	311:312	arg1	structure					273:281	structure	273:281	structure	273:281	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	59	theme	CS	311:312	arg1	state					296:300	chemical state	287:300	chemical state	287:300	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	59	theme	CS	311:312	arg1	morphology					261:270	The morphology	257:270	The morphology	257:270	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	7	60	theme	interactions	1109:1120	arg1	participation					1062:1074	the participation	1058:1074	the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects	1058:1167	The removal of CR was attributed to the participation of hydrogen bonds, electrostatic interactions, π-π conjugation and zinc coordination effects.					
31254578	1	61	theme	ZIF-8	204:208	arg1	nanocomposites					188:201	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites	126:201	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS)	126:212	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	61	theme	ZIF-8	204:208	arg1	CS					210:211	ZIF-8@CS	204:211	ZIF-8@CS	204:211	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	2	62	theme	photoelectron	417:429	arg1	XPS					445:447	XPS	445:447	XPS	445:447	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	62	theme	photoelectron	417:429	arg1	spectroscopy					431:442	X-ray photoelectron spectroscopy	411:442	X-ray photoelectron spectroscopy (XPS)	411:448	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	2	62	theme	photoelectron	417:429	arg1	microscope					353:362	scanning electron microscope	335:362	scanning electron microscope (SEM)	335:368	The morphology, structure and chemical state of ZIF-8@CS were investigated by scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), Fourier transform IR spectroscopy (FTIR) and X-ray diffraction (XRD).					
31254578	0	63	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ growth of ZIF-8 nanoparticles on chitosan to form the hybrid nanocomposites for high-efficiency removal of Congo Red.					
31254578	1	64	theme	zeolitic	130:137	arg1	CS					184:185	CS	184:185	CS	184:185	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	64	theme	zeolitic	130:137	arg1	chitosan					174:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan	126:181	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS)	126:212	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	65	theme	@	209:209	arg1	nanocomposites					188:201	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites	126:201	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS)	126:212	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31254578	1	65	theme	@	209:209	arg1	CS					210:211	ZIF-8@CS	204:211	ZIF-8@CS	204:211	The zeolitic imidazole framework (ZIF-8) dotted chitosan (CS) nanocomposites (ZIF-8@CS) were fabricated via in-situ growth method.					
31752807	12	0	theme	Garidisan	1877:1885	arg1	effect					1867:1872	The therapeutic effect	1851:1872	The therapeutic effect of Garidisan on UC	1851:1891	The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria.					
31752807	6	1	theme	UC	803:804	arg1	model					812:816	a UC mouse model	801:816	a UC mouse model	801:816	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	9	2	theme	Bayesian	1498:1505	arg1	analysis					1522:1529	Bayesian causal network analysis	1498:1529	Bayesian causal network analysis	1498:1529	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	13	3	theme	good	1998:2001	arg1	effect					2014:2019	a good regulatory effect	1996:2019	a good regulatory effect	1996:2019	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	6	4	theme	gut	783:785	arg1	microbiota					787:796	the gut microbiota	779:796	the gut microbiota	779:796	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	9	5	theme	network	1514:1520	arg1	analysis					1522:1529	Bayesian causal network analysis	1498:1529	Bayesian causal network analysis	1498:1529	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	12	6	theme	bacteria	1961:1968	arg1	family					1951:1956	the Lachnospiraceae family	1931:1956	the Lachnospiraceae family of bacteria	1931:1968	The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria.					
31752807	8	7	theme	rRNA	1121:1124	arg1	region					1139:1144	the 16S rRNA gene V3 + V4 region	1113:1144	the 16S rRNA gene V3 + V4 region	1113:1144	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	11	8	theme	significant	1652:1662	arg1	differences					1664:1674	significant differences	1652:1674	significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group	1652:1771	There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group.					
31752807	5	9	theme	Previous	559:566	arg1	studies					568:574	Previous studies	559:574	Previous studies	559:574	Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear.					
31752807	9	10	from	differences	1336:1346	arg1	structure					1395:1403	microbial community structure	1375:1403	microbial community structure	1375:1403	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	9	10	from	differences	1336:1346	arg1	species					1351:1357	species composition and microbial community structure	1351:1403	species composition and microbial community structure	1351:1403	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	9	10	from	differences	1336:1346	arg1	composition					1359:1369	composition	1359:1369	composition	1359:1369	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	8	11	theme	V3 + V4	1131:1137	arg1	region					1139:1144	the 16S rRNA gene V3 + V4 region	1113:1144	the 16S rRNA gene V3 + V4 region	1113:1144	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	0	12	theme	colitis	99:105	arg1	model					79:83	the mouse model	69:83	the mouse model of ulcerative colitis induced by dextran sulfate sodium	69:139	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	13	13	theme	UC	2134:2135	arg1	treatment					2121:2129	the treatment	2117:2129	the treatment of UC	2117:2135	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	6	14	theme	possible	835:842	arg1	mechanism					844:852	the possible mechanism	831:852	the possible mechanism of the therapeutic effect of Garidisan on UC	831:897	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	12	15	from	effect	1921:1926	arg1	family					1951:1956	the Lachnospiraceae family	1931:1956	the Lachnospiraceae family of bacteria	1931:1968	The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria.					
31752807	1	16	theme	modern	182:187	arg1	disease					200:206	a modern refractory disease	180:206	a modern refractory disease	180:206	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	1	16	theme	modern	182:187	arg1	colitis					164:170	BACKGROUND Ulcerative colitis	142:170	BACKGROUND Ulcerative colitis (UC)	142:175	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	8	17	theme	luminal	1153:1159	arg1	contents					1161:1168	the luminal contents	1149:1168	the luminal contents of the control group, model group, and Garidisan group	1149:1223	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	0	18	theme	sulfate	126:132	arg1	sodium					134:139	dextran sulfate sodium	118:139	dextran sulfate sodium	118:139	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	7	19	theme	UC	912:913	arg1	model					921:925	The UC mouse model	908:925	The UC mouse model	908:925	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	8	20	theme	control	1177:1183	arg1	group					1185:1189	the control group	1173:1189	the control group	1173:1189	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	5	21	theme	therapeutic	657:667	arg1	mechanism					669:677	the therapeutic mechanism	653:677	the therapeutic mechanism	653:677	Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear.					
31752807	5	21	theme	therapeutic	657:667	arg1	unclear					688:694	unclear	688:694	unclear	688:694	Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear.					
31752807	13	22	theme	direct	2086:2091	arg1	target					2093:2098	an important direct target	2073:2098	an important direct target of Garidisan for the treatment of UC	2073:2135	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	13	22	theme	direct	2086:2091	arg1	Lachnospiraceae					2048:2062	Lachnospiraceae	2048:2062	Lachnospiraceae	2048:2062	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	6	23	from	UC	896:897	arg1	mechanism					844:852	the possible mechanism	831:852	the possible mechanism of the therapeutic effect of Garidisan on UC	831:897	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	5	24	theme	good	608:611	arg1	effect					625:630	a good therapeutic effect	606:630	a good therapeutic effect	606:630	Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear.					
31752807	1	25	theme	BACKGROUND	142:151	arg1	UC					173:174	UC	173:174	UC	173:174	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	1	25	theme	BACKGROUND	142:151	arg1	colitis					164:170	BACKGROUND Ulcerative colitis	142:170	BACKGROUND Ulcerative colitis (UC)	142:175	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	1	25	theme	BACKGROUND	142:151	arg1	disease					200:206	a modern refractory disease	180:206	a modern refractory disease	180:206	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	8	26	theme	group	1198:1202	arg1	contents					1161:1168	the luminal contents	1149:1168	the luminal contents of the control group, model group, and Garidisan group	1149:1223	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	12	27	theme	direct	1914:1919	arg1	effect					1921:1926	the direct effect	1910:1926	the direct effect on the Lachnospiraceae family of bacteria	1910:1968	The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria.					
31752807	0	28	theme	mouse	73:77	arg1	model					79:83	the mouse model	69:83	the mouse model of ulcerative colitis induced by dextran sulfate sodium	69:139	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	6	29	theme	therapeutic	861:871	arg1	effect					873:878	the therapeutic effect	857:878	the therapeutic effect of Garidisan on UC	857:897	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	6	30	from	effect	873:878	arg1	UC					896:897	UC	896:897	UC	896:897	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	8	31	theme	group	1219:1223	arg1	contents					1161:1168	the luminal contents	1149:1168	the luminal contents of the control group, model group, and Garidisan group	1149:1223	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	7	32	dep	METHODS	900:906	arg1	established					931:941	established	931:941	was established by the dextran sulfate sodium (DSS) circulating free water drinking method	927:1016	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	7	32	dep	METHODS	900:906	arg1	sampled					1049:1055	sampled	1049:1055	were sampled under sterile conditions	1044:1080	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	3	33	theme	Artemisia	371:379	arg1	Willd					389:393	Artemisia frigida Willd	371:393	Artemisia frigida Willd	371:393	Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC in Inner Mongolia, China.					
31752807	0	34	theme	Regulatory	0:9	arg1	effect					11:16	Regulatory effect	0:16	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.	0:140	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	9	35	theme	samples	1434:1440	arg1	samples					1434:1440	samples	1434:1440	samples	1434:1440	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	9	35	theme	samples	1434:1440	arg1	groups					1424:1429	individual groups	1413:1429	individual groups of samples	1413:1440	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	6	36	from	mechanism	844:852	arg1	UC					896:897	UC	896:897	UC	896:897	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	5	37	with	mice	635:638	arg1	UC					645:646	UC	645:646	UC	645:646	Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear.					
31752807	13	38	theme	gut	2028:2030	arg1	microbiota					2032:2041	the gut microbiota	2024:2041	the gut microbiota	2024:2041	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	6	39	theme	Garidisan	753:761	arg1	effect					743:748	the regulatory effect	728:748	the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model	728:816	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	0	40	theme	gut	51:53	arg1	microbiota					55:64	the gut microbiota	47:64	the gut microbiota	47:64	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	10	41	dep	RESULTS	1532:1538	arg1	model					1553:1557	The UC mouse model	1540:1557	RESULTS The UC mouse model	1532:1557	RESULTS The UC mouse model was successfully established and the sequencing results were of adequate quality.					
31752807	7	42	theme	drinking	1002:1009	arg1	method					1011:1016	the dextran sulfate sodium (DSS) circulating free water drinking method	946:1016	the dextran sulfate sodium (DSS) circulating free water drinking method	946:1016	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	8	43	theme	taxonomic	1270:1278	arg1	OTUs					1287:1290	OTUs	1287:1290	OTUs	1287:1290	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	8	43	theme	taxonomic	1270:1278	arg1	units					1280:1284	operational taxonomic units	1258:1284	operational taxonomic units (OTUs)	1258:1291	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	10	44	theme	UC	1544:1545	arg1	model					1553:1557	The UC mouse model	1540:1557	RESULTS The UC mouse model	1532:1557	RESULTS The UC mouse model was successfully established and the sequencing results were of adequate quality.					
31752807	1	45	theme	refractory	189:198	arg1	disease					200:206	a modern refractory disease	180:206	a modern refractory disease	180:206	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	1	45	theme	refractory	189:198	arg1	colitis					164:170	BACKGROUND Ulcerative colitis	142:170	BACKGROUND Ulcerative colitis (UC)	142:175	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	9	46	theme	rank	1479:1482	arg1	test					1488:1491	rank sum test	1479:1491	rank sum test	1479:1491	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	12	47	theme	therapeutic	1855:1865	arg1	effect					1867:1872	The therapeutic effect	1851:1872	The therapeutic effect of Garidisan on UC	1851:1891	The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria.					
31752807	6	48	theme	mouse	806:810	arg1	model					812:816	a UC mouse model	801:816	a UC mouse model	801:816	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	13	49	contain	has	1992:1994	arg1	Garidisan					1982:1990	CONCLUSION Garidisan	1971:1990	CONCLUSION Garidisan	1971:1990	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	13	49	contain	has	1992:1994	arg2	effect					2014:2019	a good regulatory effect	1996:2019	a good regulatory effect	1996:2019	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	7	50	theme	circulating	979:989	arg1	method					1011:1016	the dextran sulfate sodium (DSS) circulating free water drinking method	946:1016	the dextran sulfate sodium (DSS) circulating free water drinking method	946:1016	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	11	51	theme	control	1725:1731	arg1	group					1733:1737	the control group	1721:1737	the control group	1721:1737	There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group.					
31752807	12	52	from	effect	1867:1872	arg1	UC					1890:1891	UC	1890:1891	UC	1890:1891	The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria.					
31752807	6	53	theme	microbiota	787:796	arg1	dysbiosis					766:774	dysbiosis	766:774	dysbiosis of the gut microbiota in a UC mouse model	766:816	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	9	54	theme	causal	1507:1512	arg1	analysis					1522:1529	Bayesian causal network analysis	1498:1529	Bayesian causal network analysis	1498:1529	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	10	55	theme	sequencing	1596:1605	arg1	results					1607:1613	the sequencing results	1592:1613	the sequencing results	1592:1613	RESULTS The UC mouse model was successfully established and the sequencing results were of adequate quality.					
31752807	1	56	theme	Ulcerative	153:162	arg1	UC					173:174	UC	173:174	UC	173:174	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	1	56	theme	Ulcerative	153:162	arg1	colitis					164:170	BACKGROUND Ulcerative colitis	142:170	BACKGROUND Ulcerative colitis (UC)	142:175	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	1	56	theme	Ulcerative	153:162	arg1	disease					200:206	a modern refractory disease	180:206	a modern refractory disease	180:206	BACKGROUND Ulcerative colitis (UC) is a modern refractory disease, and its etiology has been difficult to discern.					
31752807	4	57	theme	clinical	469:476	arg1	settings					478:485	clinical settings	469:485	clinical settings	469:485	In clinical settings, Garidisan has been found to treat UC effectively, with low recurrence.					
31752807	11	58	from	differences	1664:1674	arg1	diversity					1683:1691	the diversity	1679:1691	the diversity of luminal contents	1679:1711	There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group.					
31752807	8	59	theme	16S	1117:1119	arg1	region					1139:1144	the 16S rRNA gene V3 + V4 region	1113:1144	the 16S rRNA gene V3 + V4 region	1113:1144	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	7	60	dep	circulating	979:989	arg1	free					991:994	free	991:994	free	991:994	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	5	61	theme	therapeutic	613:623	arg1	effect					625:630	a good therapeutic effect	606:630	a good therapeutic effect	606:630	Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear.					
31752807	10	62	theme	adequate	1623:1630	arg1	quality					1632:1638	adequate quality	1623:1638	adequate quality	1623:1638	RESULTS The UC mouse model was successfully established and the sequencing results were of adequate quality.					
31752807	9	63	theme	community	1385:1393	arg1	structure					1395:1403	microbial community structure	1375:1403	microbial community structure	1375:1403	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	9	63	theme	community	1385:1393	arg1	species					1351:1357	species composition and microbial community structure	1351:1403	species composition and microbial community structure	1351:1403	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	8	64	theme	gene	1126:1129	arg1	region					1139:1144	the 16S rRNA gene V3 + V4 region	1113:1144	the 16S rRNA gene V3 + V4 region	1113:1144	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	3	65	theme	UC	437:438	arg1	treatment					424:432	the treatment	420:432	the treatment of UC	420:438	Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC in Inner Mongolia, China.					
31752807	0	66	theme	dextran	118:124	arg1	sodium					134:139	dextran sulfate sodium	118:139	dextran sulfate sodium	118:139	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	8	67	theme	species	1297:1303	arg1	annotation					1305:1314	species annotation	1297:1314	species annotation	1297:1314	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	6	68	from	dysbiosis	766:774	arg1	model					812:816	a UC mouse model	801:816	a UC mouse model	801:816	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	8	69	theme	region	1139:1144	arg1	sequencing					1099:1108	High-throughput sequencing	1083:1108	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group	1083:1223	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	6	70	from	effect	743:748	arg1	dysbiosis					766:774	dysbiosis	766:774	dysbiosis of the gut microbiota in a UC mouse model	766:816	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	7	71	theme	mouse	915:919	arg1	model					921:925	The UC mouse model	908:925	The UC mouse model	908:925	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	13	72	theme	CONCLUSION	1971:1980	arg1	Garidisan					1982:1990	CONCLUSION Garidisan	1971:1990	CONCLUSION Garidisan	1971:1990	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	13	73	theme	Garidisan	2103:2111	arg1	target					2093:2098	an important direct target	2073:2098	an important direct target of Garidisan for the treatment of UC	2073:2135	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	13	73	theme	Garidisan	2103:2111	arg1	Lachnospiraceae					2048:2062	Lachnospiraceae	2048:2062	Lachnospiraceae	2048:2062	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	8	74	theme	High-throughput	1083:1097	arg1	sequencing					1099:1108	High-throughput sequencing	1083:1108	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group	1083:1223	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	8	75	theme	contents	1161:1168	arg1	sequencing					1099:1108	High-throughput sequencing	1083:1108	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group	1083:1223	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	11	76	theme	luminal	1696:1702	arg1	contents					1704:1711	luminal contents	1696:1711	luminal contents	1696:1711	There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group.					
31752807	0	77	theme	microbiota	55:64	arg1	dysbiosis					34:42	dysbiosis	34:42	dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium	34:139	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	13	78	theme	important	2076:2084	arg1	target					2093:2098	an important direct target	2073:2098	an important direct target of Garidisan for the treatment of UC	2073:2135	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	13	78	theme	important	2076:2084	arg1	Lachnospiraceae					2048:2062	Lachnospiraceae	2048:2062	Lachnospiraceae	2048:2062	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	13	79	theme	regulatory	2003:2012	arg1	effect					2014:2019	a good regulatory effect	1996:2019	a good regulatory effect	1996:2019	CONCLUSION Garidisan has a good regulatory effect on the gut microbiota, and Lachnospiraceae could be an important direct target of Garidisan for the treatment of UC.					
31752807	7	80	theme	sterile	1063:1069	arg1	conditions					1071:1080	sterile conditions	1063:1080	sterile conditions	1063:1080	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	9	81	theme	microbial	1375:1383	arg1	structure					1395:1403	microbial community structure	1375:1403	microbial community structure	1375:1403	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	9	81	theme	microbial	1375:1383	arg1	species					1351:1357	species composition and microbial community structure	1351:1403	species composition and microbial community structure	1351:1403	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	6	82	theme	Garidisan	883:891	arg1	effect					873:878	the therapeutic effect	857:878	the therapeutic effect of Garidisan on UC	857:897	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	0	83	from	effect	11:16	arg1	dysbiosis					34:42	dysbiosis	34:42	dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium	34:139	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	8	84	theme	group	1185:1189	arg1	contents					1161:1168	the luminal contents	1149:1168	the luminal contents of the control group, model group, and Garidisan group	1149:1223	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	4	85	located	found	507:511	arg2	Garidisan					488:496	Garidisan	488:496	Garidisan	488:496	In clinical settings, Garidisan has been found to treat UC effectively, with low recurrence.					
31752807	4	85	located	found	507:511	arg1	settings					478:485	clinical settings	469:485	clinical settings	469:485	In clinical settings, Garidisan has been found to treat UC effectively, with low recurrence.					
31752807	6	86	theme	effect	873:878	arg1	mechanism					844:852	the possible mechanism	831:852	the possible mechanism of the therapeutic effect of Garidisan on UC	831:897	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	9	87	dep	species	1351:1357	arg1	structure					1395:1403	microbial community structure	1375:1403	microbial community structure	1375:1403	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	9	87	dep	species	1351:1357	arg1	species					1351:1357	species composition and microbial community structure	1351:1403	species composition and microbial community structure	1351:1403	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	9	87	dep	species	1351:1357	arg1	composition					1359:1369	composition	1359:1369	composition	1359:1369	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	8	88	theme	model	1192:1196	arg1	group					1198:1202	model group	1192:1202	model group	1192:1202	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	11	89	theme	contents	1704:1711	arg1	diversity					1683:1691	the diversity	1679:1691	the diversity of luminal contents	1679:1711	There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group.					
31752807	0	90	theme	ulcerative	88:97	arg1	colitis					99:105	ulcerative colitis	88:105	ulcerative colitis induced by dextran sulfate sodium	88:139	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	4	91	theme	low	543:545	arg1	recurrence					547:556	low recurrence	543:556	low recurrence	543:556	In clinical settings, Garidisan has been found to treat UC effectively, with low recurrence.					
31752807	9	92	theme	individual	1413:1422	arg1	samples					1434:1440	samples	1434:1440	samples	1434:1440	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	9	92	theme	individual	1413:1422	arg1	groups					1424:1429	individual groups	1413:1429	individual groups of samples	1413:1440	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	0	93	from	dysbiosis	34:42	arg1	model					79:83	the mouse model	69:83	the mouse model of ulcerative colitis induced by dextran sulfate sodium	69:139	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	11	94	theme	Garidisan	1757:1765	arg1	group					1767:1771	Garidisan group	1757:1771	Garidisan group	1757:1771	There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group.					
31752807	7	95	theme	luminal	1027:1033	arg1	contents					1035:1042	the luminal contents	1023:1042	the luminal contents	1023:1042	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	8	96	theme	Garidisan	1209:1217	arg1	group					1219:1223	Garidisan group	1209:1223	Garidisan group	1209:1223	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	3	97	theme	frigida	381:387	arg1	Willd					389:393	Artemisia frigida Willd	371:393	Artemisia frigida Willd	371:393	Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC in Inner Mongolia, China.					
31752807	0	98	theme	Garidisan	21:29	arg1	effect					11:16	Regulatory effect	0:16	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.	0:140	Regulatory effect of Garidisan on dysbiosis of the gut microbiota in the mouse model of ulcerative colitis induced by dextran sulfate sodium.					
31752807	3	99	used	used	411:414	arg2	Garidisan					331:339	Garidisan	331:339	Garidisan	331:339	Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC in Inner Mongolia, China.					
31752807	7	100	theme	water	996:1000	arg1	method					1011:1016	the dextran sulfate sodium (DSS) circulating free water drinking method	946:1016	the dextran sulfate sodium (DSS) circulating free water drinking method	946:1016	METHODS The UC mouse model was established by the dextran sulfate sodium (DSS) circulating free water drinking method, and the luminal contents were sampled under sterile conditions.					
31752807	3	101	theme	wild	356:359	arg1	poppy					361:365	wild poppy	356:365	wild poppy	356:365	Garidisan is composed of wild poppy and Artemisia frigida Willd and is commonly used for the treatment of UC in Inner Mongolia, China.					
31752807	5	102	contain	has	602:604	arg1	Garidisan					592:600	Garidisan	592:600	Garidisan	592:600	Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear.					
31752807	5	102	contain	has	602:604	arg2	effect					625:630	a good therapeutic effect	606:630	a good therapeutic effect	606:630	Previous studies have shown that Garidisan has a good therapeutic effect on mice with UC, but the therapeutic mechanism is still unclear.					
31752807	10	103	theme	mouse	1547:1551	arg1	model					1553:1557	The UC mouse model	1540:1557	RESULTS The UC mouse model	1532:1557	RESULTS The UC mouse model was successfully established and the sequencing results were of adequate quality.					
31752807	6	104	theme	regulatory	732:741	arg1	effect					743:748	the regulatory effect	728:748	the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model	728:816	In this study, we investigated the regulatory effect of Garidisan on dysbiosis of the gut microbiota in a UC mouse model and explored the possible mechanism of the therapeutic effect of Garidisan on UC.					
31752807	8	105	theme	operational	1258:1268	arg1	OTUs					1287:1290	OTUs	1287:1290	OTUs	1287:1290	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	8	105	theme	operational	1258:1268	arg1	units					1280:1284	operational taxonomic units	1258:1284	operational taxonomic units (OTUs)	1258:1291	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	11	106	theme	model	1740:1744	arg1	group					1746:1750	model group	1740:1750	model group	1740:1750	There were significant differences in the diversity of luminal contents between the control group, model group, and Garidisan group, and the differences between groups were greater than those within any group.					
31752807	12	107	theme	Lachnospiraceae	1935:1949	arg1	family					1951:1956	the Lachnospiraceae family	1931:1956	the Lachnospiraceae family of bacteria	1931:1968	The therapeutic effect of Garidisan on UC is attributed to the direct effect on the Lachnospiraceae family of bacteria.					
31752807	9	108	theme	sum	1484:1486	arg1	test					1488:1491	rank sum test	1479:1491	rank sum test	1479:1491	The differences in species composition and microbial community structure between individual groups of samples were analyzed using MetaStat, LefSe, rank sum test, and Bayesian causal network analysis.					
31752807	8	109	theme	units	1280:1284	arg1	clustering					1244:1253	clustering	1244:1253	clustering of operational taxonomic units (OTUs)	1244:1291	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	8	109	theme	units	1280:1284	arg1	annotation					1305:1314	species annotation	1297:1314	species annotation	1297:1314	High-throughput sequencing of the 16S rRNA gene V3 + V4 region of the luminal contents of the control group, model group, and Garidisan group was conducted, and clustering of operational taxonomic units (OTUs) and species annotation were performed.					
31752807	2	110	theme	gut	315:317	arg1	microbiota					319:328	the gut microbiota	311:328	the gut microbiota	311:328	Studies have shown that UC is closely associated with the gut microbiota.					
29866005	4	0	theme	Different	889:897	arg1	ratios					907:912	Different polymer ratios	889:912	Different polymer ratios in formulation	889:927	Different polymer ratios in formulation showed significant influence on T50% and dynamic swelling of hydrogel.					
29866005	1	1	theme	interpenetrating	299:314	arg1	films					333:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films	253:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique	253:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	2	2	theme	formulation	633:643	arg1	selection					645:653	optimum formulation selection	625:653	optimum formulation selection	625:653	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	2	3	used	used	515:518	arg2	plots					504:508	the surface plots	492:508	the surface plots	492:508	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	2	3	used	used	515:518	arg2	analysis					479:486	regression analysis	468:486	regression analysis	468:486	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	1	4	theme	network	325:331	arg1	films					333:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films	253:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique	253:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	1	5	link	cross-linked	261:272	arg1	films					333:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films	253:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique	253:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	1	6	theme	composite	199:207	arg1	design					209:214	central composite design	191:214	central composite design	191:214	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	8	7	from	distribution	1434:1445	arg1	system					1474:1479	hydrogel matrix system	1458:1479	hydrogel matrix system	1458:1479	CONCLUSION It indicated the uniform distribution of drug in hydrogel matrix system.					
29866005	3	8	from	pH	881:882	arg1	fluid					872:876	the simulated intestinal fluid	847:876	the simulated intestinal fluid at pH 7.4	847:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	3	8	from	pH	881:882	arg1	hours					838:842	the next 10 hours	826:842	the next 10 hours in the simulated intestinal fluid at pH 7.4	826:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	3	9	theme	gastric	782:788	arg1	pH					790:791	simulated gastric pH 1.2	772:795	simulated gastric pH 1.2	772:795	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	0	10	theme	Surface	144:150	arg1	Methodology					152:162	Response Surface Methodology	135:162	Response Surface Methodology	135:162	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	10	11	theme	crosslinking	1760:1771	arg1	agent					1773:1777	crosslinking agent	1760:1777	crosslinking agent	1760:1777	Acute oral toxicity study was performed to investigate the toxic effect of crosslinking agent and polymer used in formulations.					
29866005	2	12	theme	drug	585:588	arg1	release					590:596	drug release	585:596	drug release	585:596	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	7	13	theme	scanning	1368:1375	arg1	microscope					1386:1395	scanning electron microscope	1368:1395	scanning electron microscope	1368:1395	The physical state of hydrogel was investigated by scanning electron microscope.					
29866005	4	14	from	swelling	978:985	arg1	%					964:964	T50%	961:964	T50%	961:964	Different polymer ratios in formulation showed significant influence on T50% and dynamic swelling of hydrogel.					
29866005	6	15	theme	drug	1223:1226	arg1	rate					1236:1239	drug release rate	1223:1239	drug release rate	1223:1239	Cross-linker also affects drug release rate, i.e. the release rate is decreased with the increase in its concentration.					
29866005	2	16	theme	dynamic	603:609	arg1	swelling					611:618	dynamic swelling	603:618	dynamic swelling	603:618	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	3	17	from	hours	748:752	arg1	release					811:817	then slow release	801:817	then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4	801:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	3	17	from	hours	748:752	arg1	pH					790:791	simulated gastric pH 1.2	772:795	simulated gastric pH 1.2	772:795	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	5	18	theme	dynamic	1047:1053	arg1	swelling					1055:1062	dynamic swelling	1047:1062	dynamic swelling at 7.86 h. RESULT It	1047:1083	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	4	19	theme	significant	936:946	arg1	influence					948:956	significant influence	936:956	significant influence on T50%	936:964	Different polymer ratios in formulation showed significant influence on T50% and dynamic swelling of hydrogel.					
29866005	8	20	theme	matrix	1467:1472	arg1	system					1474:1479	hydrogel matrix system	1458:1479	hydrogel matrix system	1458:1479	CONCLUSION It indicated the uniform distribution of drug in hydrogel matrix system.					
29866005	7	21	theme	hydrogel	1339:1346	arg1	state					1330:1334	The physical state	1317:1334	The physical state of hydrogel	1317:1346	The physical state of hydrogel was investigated by scanning electron microscope.					
29866005	5	22	located	observed	1021:1028	arg1	hour					1038:1041	9.89 hour	1033:1041	9.89 hour	1033:1041	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	5	22	located	observed	1021:1028	arg2	%					1015:1015	The highest T50%	1000:1015	The highest T50%	1000:1015	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	4	23	from	ratios	907:912	arg1	formulation					917:927	formulation	917:927	formulation	917:927	Different polymer ratios in formulation showed significant influence on T50% and dynamic swelling of hydrogel.					
29866005	3	24	theme	burst	664:668	arg1	release					670:676	Initial burst release	656:676	Initial burst release of drug	656:684	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	8	25	dep	CONCLUSION	1398:1407	arg1	It					1409:1410	It	1409:1410	It	1409:1410	CONCLUSION It indicated the uniform distribution of drug in hydrogel matrix system.					
29866005	3	26	theme	simulated	851:859	arg1	fluid					872:876	the simulated intestinal fluid	847:876	the simulated intestinal fluid at pH 7.4	847:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	2	27	with	%	562:562	arg1	selection					645:653	optimum formulation selection	625:653	optimum formulation selection	625:653	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	2	28	theme	optimum	625:631	arg1	selection					645:653	optimum formulation selection	625:653	optimum formulation selection	625:653	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	4	29	theme	dynamic	970:976	arg1	swelling					978:985	dynamic swelling	970:985	dynamic swelling of hydrogel	970:997	Different polymer ratios in formulation showed significant influence on T50% and dynamic swelling of hydrogel.					
29866005	3	30	from	release	811:817	arg1	dissolution					757:767	dissolution	757:767	dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4	757:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	3	30	from	release	811:817	arg1	hours					748:752	the first 2 hours	736:752	the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4	736:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	0	31	theme	Release	115:121	arg1	Study					123:127	Metformin Release Study	105:127	Metformin Release Study Using Response Surface Methodology	105:162	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	2	32	from	effect	536:541	arg1	swelling					611:618	dynamic swelling	603:618	dynamic swelling	603:618	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	2	32	from	effect	536:541	arg1	%					562:562	T50%	559:562	T50% (the time for 50% of drug release)	559:597	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	5	33	theme	highest	1004:1010	arg1	%					1015:1015	The highest T50%	1000:1015	The highest T50%	1000:1015	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	7	34	theme	physical	1321:1328	arg1	state					1330:1334	The physical state	1317:1334	The physical state of hydrogel	1317:1346	The physical state of hydrogel was investigated by scanning electron microscope.					
29866005	2	35	theme	regression	468:477	arg1	analysis					479:486	regression analysis	468:486	regression analysis	468:486	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	1	36	theme	films	333:337	arg1	optimization					237:248	the optimization	233:248	the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique	233:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	9	37	theme	hydrogel	1597:1604	arg1	network					1606:1612	interpenetrating hydrogel network	1580:1612	interpenetrating hydrogel network	1580:1612	Moreover, the presence of hydrogen and ionic bonds between polymers and crosslinking agent formed interpenetrating hydrogel network, likely responsible for increased value of T50%, as confirmed by FTIR.					
29866005	2	38	theme	surface	496:502	arg1	plots					504:508	the surface plots	492:508	the surface plots	492:508	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	0	39	theme	pH-Sensitive	34:45	arg1	Hydrogel					92:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	1	40	theme	evaporation	366:376	arg1	technique					378:386	solvent evaporation technique	358:386	solvent evaporation technique	358:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	6	41	from	increase	1286:1293	arg1	concentration					1302:1314	its concentration	1298:1314	its concentration	1298:1314	Cross-linker also affects drug release rate, i.e. the release rate is decreased with the increase in its concentration.					
29866005	0	42	theme	L100	87:90	arg1	Hydrogel					92:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	0	43	theme	Cross-Linked	55:66	arg1	Hydrogel					92:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	5	44	theme	release	1152:1158	arg1	rate					1160:1163	release rate	1152:1163	release rate of metformin	1152:1176	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	9	45	theme	responsible	1622:1632	arg1	network					1606:1612	interpenetrating hydrogel network	1580:1612	interpenetrating hydrogel network	1580:1612	Moreover, the presence of hydrogen and ionic bonds between polymers and crosslinking agent formed interpenetrating hydrogel network, likely responsible for increased value of T50%, as confirmed by FTIR.					
29866005	10	46	theme	oral	1691:1694	arg1	study					1705:1709	Acute oral toxicity study	1685:1709	Acute oral toxicity study	1685:1709	Acute oral toxicity study was performed to investigate the toxic effect of crosslinking agent and polymer used in formulations.					
29866005	9	47	theme	increased	1638:1646	arg1	value					1648:1652	increased value	1638:1652	increased value of T50%	1638:1660	Moreover, the presence of hydrogen and ionic bonds between polymers and crosslinking agent formed interpenetrating hydrogel network, likely responsible for increased value of T50%, as confirmed by FTIR.					
29866005	3	48	theme	formulated	708:717	arg1	hydrogels					719:727	the formulated hydrogels	704:727	the formulated hydrogels	704:727	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	1	49	theme	cross-linked	261:272	arg1	films					333:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films	253:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique	253:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	2	50	theme	response	438:445	arg1	methodology					455:465	response surface methodology	438:465	response surface methodology	438:465	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	9	51	theme	%	1660:1660	arg1	value					1648:1652	increased value	1638:1652	increased value of T50%	1638:1660	Moreover, the presence of hydrogen and ionic bonds between polymers and crosslinking agent formed interpenetrating hydrogel network, likely responsible for increased value of T50%, as confirmed by FTIR.					
29866005	3	52	theme	first	740:744	arg1	hours					748:752	the first 2 hours	736:752	the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4	736:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	2	53	with	swelling	611:618	arg1	selection					645:653	optimum formulation selection	625:653	optimum formulation selection	625:653	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	1	54	theme	hydrogel	316:323	arg1	films					333:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films	253:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique	253:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	10	55	theme	polymer	1783:1789	arg1	effect					1750:1755	the toxic effect	1740:1755	the toxic effect of crosslinking agent and polymer used in formulations	1740:1810	Acute oral toxicity study was performed to investigate the toxic effect of crosslinking agent and polymer used in formulations.					
29866005	4	56	theme	polymer	899:905	arg1	ratios					907:912	Different polymer ratios	889:912	Different polymer ratios in formulation	889:927	Different polymer ratios in formulation showed significant influence on T50% and dynamic swelling of hydrogel.					
29866005	3	57	theme	simulated	772:780	arg1	pH					790:791	simulated gastric pH 1.2	772:795	simulated gastric pH 1.2	772:795	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	6	58	theme	release	1251:1257	arg1	rate					1259:1262	the release rate	1247:1262	the release rate	1247:1262	Cross-linker also affects drug release rate, i.e. the release rate is decreased with the increase in its concentration.					
29866005	10	59	theme	toxic	1744:1748	arg1	effect					1750:1755	the toxic effect	1740:1755	the toxic effect of crosslinking agent and polymer used in formulations	1740:1810	Acute oral toxicity study was performed to investigate the toxic effect of crosslinking agent and polymer used in formulations.					
29866005	1	60	theme	central	191:197	arg1	design					209:214	central composite design	191:214	central composite design	191:214	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	3	61	theme	dissolution	757:767	arg1	hours					748:752	the first 2 hours	736:752	the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4	736:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	2	62	theme	METHODS	389:395	arg1	formulations					406:417	METHODS Hydrogel formulations	389:417	METHODS Hydrogel formulations	389:417	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	10	63	theme	agent	1773:1777	arg1	effect					1750:1755	the toxic effect	1740:1755	the toxic effect of crosslinking agent and polymer used in formulations	1740:1810	Acute oral toxicity study was performed to investigate the toxic effect of crosslinking agent and polymer used in formulations.					
29866005	2	64	theme	release	590:596	arg1	release					590:596	drug release	585:596	drug release	585:596	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	2	64	theme	release	590:596	arg1	%					580:580	50%	578:580	50% of drug release	578:596	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	8	65	theme	drug	1450:1453	arg1	distribution					1434:1445	the uniform distribution	1422:1445	the uniform distribution of drug in hydrogel matrix system	1422:1479	CONCLUSION It indicated the uniform distribution of drug in hydrogel matrix system.					
29866005	9	66	theme	bonds	1527:1531	arg1	presence					1496:1503	the presence	1492:1503	the presence of hydrogen and ionic bonds between polymers and crosslinking agent	1492:1571	Moreover, the presence of hydrogen and ionic bonds between polymers and crosslinking agent formed interpenetrating hydrogel network, likely responsible for increased value of T50%, as confirmed by FTIR.					
29866005	8	67	theme	hydrogel	1458:1465	arg1	system					1474:1479	hydrogel matrix system	1458:1479	hydrogel matrix system	1458:1479	CONCLUSION It indicated the uniform distribution of drug in hydrogel matrix system.					
29866005	7	68	theme	electron	1377:1384	arg1	microscope					1386:1395	scanning electron microscope	1368:1395	scanning electron microscope	1368:1395	The physical state of hydrogel was investigated by scanning electron microscope.					
29866005	4	69	from	influence	948:956	arg1	%					964:964	T50%	961:964	T50%	961:964	Different polymer ratios in formulation showed significant influence on T50% and dynamic swelling of hydrogel.					
29866005	1	70	used	utilized	220:227	arg2	design					209:214	central composite design	191:214	central composite design	191:214	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	3	71	theme	slow	806:809	arg1	release					811:817	then slow release	801:817	then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4	801:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	0	72	theme	Hydrogel	92:99	arg1	Characterization					14:29	Characterization	14:29	Characterization	14:29	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	0	72	theme	Hydrogel	92:99	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	3	73	theme	Initial	656:662	arg1	release					670:676	Initial burst release	656:676	Initial burst release of drug	656:684	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	9	74	theme	hydrogen	1508:1515	arg1	bonds					1527:1531	hydrogen and ionic bonds	1508:1531	bonds	1527:1531	Moreover, the presence of hydrogen and ionic bonds between polymers and crosslinking agent formed interpenetrating hydrogel network, likely responsible for increased value of T50%, as confirmed by FTIR.					
29866005	4	75	theme	hydrogel	990:997	arg1	swelling					978:985	dynamic swelling	970:985	dynamic swelling of hydrogel	970:997	Different polymer ratios in formulation showed significant influence on T50% and dynamic swelling of hydrogel.					
29866005	4	75	theme	hydrogel	990:997	arg1	influence					948:956	significant influence	936:956	significant influence on T50%	936:964	Different polymer ratios in formulation showed significant influence on T50% and dynamic swelling of hydrogel.					
29866005	3	76	theme	next	830:833	arg1	hours					838:842	the next 10 hours	826:842	the next 10 hours in the simulated intestinal fluid at pH 7.4	826:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	0	77	theme	Metformin	105:113	arg1	Release					115:121	Metformin Release	105:121	Metformin Release Study Using Response Surface Methodology	105:162	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	5	78	theme	polymer	1119:1125	arg1	ratio					1127:1131	the polymer ratio	1115:1131	the polymer ratio	1115:1131	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	9	79	theme	ionic	1521:1525	arg1	bonds					1527:1531	hydrogen and ionic bonds	1508:1531	bonds	1527:1531	Moreover, the presence of hydrogen and ionic bonds between polymers and crosslinking agent formed interpenetrating hydrogel network, likely responsible for increased value of T50%, as confirmed by FTIR.					
29866005	3	80	theme	intestinal	861:870	arg1	fluid					872:876	the simulated intestinal fluid	847:876	the simulated intestinal fluid at pH 7.4	847:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	1	81	dep	BACKGROUND	165:174	arg1	utilized					220:227	utilized	220:227	was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique	216:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	10	82	theme	Acute	1685:1689	arg1	study					1705:1709	Acute oral toxicity study	1685:1709	Acute oral toxicity study	1685:1709	Acute oral toxicity study was performed to investigate the toxic effect of crosslinking agent and polymer used in formulations.					
29866005	0	83	theme	Response	135:142	arg1	Methodology					152:162	Response Surface Methodology	135:162	Response Surface Methodology	135:162	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	3	84	from	pH	790:791	arg1	dissolution					757:767	dissolution	757:767	dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4	757:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	3	84	from	pH	790:791	arg1	hours					748:752	the first 2 hours	736:752	the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4	736:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	5	85	from	h.	1072:1073	arg1	swelling					1055:1062	dynamic swelling	1047:1062	dynamic swelling at 7.86 h. RESULT It	1047:1083	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	9	86	theme	crosslinking	1554:1565	arg1	agent					1567:1571	crosslinking agent	1554:1571	crosslinking agent	1554:1571	Moreover, the presence of hydrogen and ionic bonds between polymers and crosslinking agent formed interpenetrating hydrogel network, likely responsible for increased value of T50%, as confirmed by FTIR.					
29866005	5	87	theme	metformin	1168:1176	arg1	rate					1160:1163	release rate	1152:1163	release rate of metformin	1152:1176	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	9	88	theme	interpenetrating	1580:1595	arg1	network					1606:1612	interpenetrating hydrogel network	1580:1612	interpenetrating hydrogel network	1580:1612	Moreover, the presence of hydrogen and ionic bonds between polymers and crosslinking agent formed interpenetrating hydrogel network, likely responsible for increased value of T50%, as confirmed by FTIR.					
29866005	0	89	theme	Genipin	47:53	arg1	Hydrogel					92:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	1	90	theme	solvent	358:364	arg1	technique					378:386	solvent evaporation technique	358:386	solvent evaporation technique	358:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	2	91	theme	variables	546:554	arg1	effect					536:541	the effect	532:541	the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection	532:653	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	3	92	theme	drug	681:684	arg1	release					670:676	Initial burst release	656:676	Initial burst release of drug	656:684	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	0	93	theme	Chitosan/Eudragit®	68:85	arg1	Hydrogel					92:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel	34:99	Synthesis and Characterization of pH-Sensitive Genipin Cross-Linked Chitosan/Eudragit® L100 Hydrogel for Metformin Release Study Using Response Surface Methodology.					
29866005	2	94	dep	%	562:562	arg1	time					569:572	the time	565:572	the time for 50% of drug release	565:596	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	10	95	theme	toxicity	1696:1703	arg1	study					1705:1709	Acute oral toxicity study	1685:1709	Acute oral toxicity study	1685:1709	Acute oral toxicity study was performed to investigate the toxic effect of crosslinking agent and polymer used in formulations.					
29866005	5	96	theme	T50	1012:1014	arg1	%					1015:1015	The highest T50%	1000:1015	The highest T50%	1000:1015	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	6	97	theme	release	1228:1234	arg1	rate					1236:1239	drug release rate	1223:1239	drug release rate	1223:1239	Cross-linker also affects drug release rate, i.e. the release rate is decreased with the increase in its concentration.					
29866005	3	98	from	hours	838:842	arg1	pH					881:882	pH 7.4	881:886	pH 7.4	881:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	3	98	from	hours	838:842	arg1	fluid					872:876	the simulated intestinal fluid	847:876	the simulated intestinal fluid at pH 7.4	847:886	Initial burst release of drug was observed from the formulated hydrogels during the first 2 hours of dissolution at simulated gastric pH 1.2 and then slow release during the next 10 hours in the simulated intestinal fluid at pH 7.4.					
29866005	2	99	theme	Hydrogel	397:404	arg1	formulations					406:417	METHODS Hydrogel formulations	389:417	METHODS Hydrogel formulations	389:417	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	1	100	theme	genipin	253:259	arg1	films					333:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films	253:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique	253:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
29866005	5	101	dep	h.	1072:1073	arg1	RESULT					1075:1080	RESULT	1075:1080	RESULT It	1075:1083	The highest T50% was observed at 9.89 hour and dynamic swelling at 7.86 h. RESULT It was observed that by changing the polymer ratio with cross-linker, release rate of metformin could be modified.					
29866005	8	102	theme	uniform	1426:1432	arg1	distribution					1434:1445	the uniform distribution	1422:1445	the uniform distribution of drug in hydrogel matrix system	1422:1479	CONCLUSION It indicated the uniform distribution of drug in hydrogel matrix system.					
29866005	2	103	theme	surface	447:453	arg1	methodology					455:465	response surface methodology	438:465	response surface methodology	438:465	METHODS Hydrogel formulations were studied using response surface methodology; regression analysis and the surface plots were used to evaluate the effect of variables on T50% (the time for 50% of drug release) and dynamic swelling with optimum formulation selection.					
29866005	1	104	theme	chitosan/Eudragit®-L	274:293	arg1	films					333:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films	253:337	genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique	253:386	BACKGROUND In this study, central composite design was utilized for the optimization of genipin cross-linked chitosan/Eudragit®-L 100 interpenetrating hydrogel network films fabricated through solvent evaporation technique.					
31427021	4	0	theme	mechanical	693:702	arg1	properties					704:713	good mechanical properties	688:713	good mechanical properties	688:713	Rheological analysis demonstrated that the nanocomposites have good mechanical properties with gelation temperature close to the body temperature; hence, they can be easily administrated locally on wounded skins.					
31427021	3	1	theme	Silver	476:481	arg1	nanoparticles					483:495	Silver nanoparticles	476:495	Silver nanoparticles (Ag NPs)	476:504	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	3	1	theme	Silver	476:481	arg1	NPs					501:503	Ag NPs	498:503	Ag NPs	498:503	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	0	2	theme	silk	91:94	arg1	nanosilver					125:134	nanosilver	125:134	nanosilver for potential wound healing	125:162	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	0	2	theme	silk	91:94	arg1	extract					96:102	corn silk extract	86:102	corn silk extract	86:102	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	0	2	theme	silk	91:94	arg1	acid					116:119	hyaluronic acid	105:119	hyaluronic acid	105:119	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	6	3	theme	Cytotoxicity	932:943	arg1	assay					945:949	Cytotoxicity assay	932:949	Cytotoxicity assay	932:949	Cytotoxicity assay showed that the hydrogels have good biocompatibility.					
31427021	5	4	theme	gram-negative	908:920	arg1	bacteria					922:929	gram-positive and gram-negative bacteria	890:929	gram-positive and gram-negative bacteria	890:929	The samples exhibited antibacterial activity toward gram-positive and gram-negative bacteria.					
31427021	1	5	theme	wounds	169:174	arg1	closure					176:182	The wounds closure	165:182	The wounds closure after physical injury or surgery	165:215	The wounds closure after physical injury or surgery is of significant clinical and research importance.					
31427021	1	5	theme	wounds	169:174	arg1	importance					257:266	importance	257:266	importance	257:266	The wounds closure after physical injury or surgery is of significant clinical and research importance.					
31427021	0	6	theme	hyaluronic	105:114	arg1	extract					96:102	corn silk extract	86:102	corn silk extract	86:102	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	0	6	theme	hyaluronic	105:114	arg1	acid					116:119	hyaluronic acid	105:119	hyaluronic acid	105:119	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	4	7	contain	have	683:686	arg1	nanocomposites					668:681	the nanocomposites	664:681	the nanocomposites	664:681	Rheological analysis demonstrated that the nanocomposites have good mechanical properties with gelation temperature close to the body temperature; hence, they can be easily administrated locally on wounded skins.					
31427021	4	7	contain	have	683:686	arg2	properties					704:713	good mechanical properties	688:713	good mechanical properties	688:713	Rheological analysis demonstrated that the nanocomposites have good mechanical properties with gelation temperature close to the body temperature; hence, they can be easily administrated locally on wounded skins.					
31427021	8	8	theme	potential	1187:1195	arg1	application					1197:1207	the potential application	1183:1207	the potential application of these novel injectable hydrogels	1183:1243	The obtained results highlight the potential application of these novel injectable hydrogels as wound dressing.					
31427021	2	9	theme	thermosensitive	284:298	arg1	hydrogels					315:323	thermosensitive and injectable hydrogels	284:323	thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver	284:393	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	4	10	theme	gelation	720:727	arg1	temperature					729:739	gelation temperature	720:739	gelation temperature close to the body temperature	720:769	Rheological analysis demonstrated that the nanocomposites have good mechanical properties with gelation temperature close to the body temperature; hence, they can be easily administrated locally on wounded skins.					
31427021	4	11	theme	good	688:691	arg1	properties					704:713	good mechanical properties	688:713	good mechanical properties	688:713	Rheological analysis demonstrated that the nanocomposites have good mechanical properties with gelation temperature close to the body temperature; hence, they can be easily administrated locally on wounded skins.					
31427021	0	12	theme	hydrogels	67:75	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	0	12	theme	hydrogels	67:75	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis	0:11	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	0	13	theme	thermosensitive	51:65	arg1	hydrogels					67:75	antibacterial thermosensitive hydrogels	37:75	antibacterial thermosensitive hydrogels	37:75	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	8	14	theme	novel	1218:1222	arg1	hydrogels					1235:1243	these novel injectable hydrogels	1212:1243	these novel injectable hydrogels	1212:1243	The obtained results highlight the potential application of these novel injectable hydrogels as wound dressing.					
31427021	5	15	theme	antibacterial	860:872	arg1	activity					874:881	antibacterial activity	860:881	antibacterial activity toward gram-positive and gram-negative bacteria	860:929	The samples exhibited antibacterial activity toward gram-positive and gram-negative bacteria.					
31427021	8	16	theme	injectable	1224:1233	arg1	hydrogels					1235:1243	these novel injectable hydrogels	1212:1243	these novel injectable hydrogels	1212:1243	The obtained results highlight the potential application of these novel injectable hydrogels as wound dressing.					
31427021	3	17	theme	Ag	498:499	arg1	nanoparticles					483:495	Silver nanoparticles	476:495	Silver nanoparticles (Ag NPs)	476:504	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	3	17	theme	Ag	498:499	arg1	NPs					501:503	Ag NPs	498:503	Ag NPs	498:503	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	3	18	theme	microwave-assisted	531:548	arg1	technique					556:564	a microwave-assisted green technique	529:564	a microwave-assisted green technique using corn silk extract in an organic solvent-free medium	529:622	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	1	19	theme	physical	190:197	arg1	injury					199:204	physical injury	190:204	physical injury	190:204	The wounds closure after physical injury or surgery is of significant clinical and research importance.					
31427021	2	20	theme	potential	419:427	arg1	use					429:431	their potential use	413:431	their potential use as a wound care material	413:456	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	3	21	theme	green	550:554	arg1	technique					556:564	a microwave-assisted green technique	529:564	a microwave-assisted green technique using corn silk extract in an organic solvent-free medium	529:622	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	4	22	theme	body	754:757	arg1	temperature					759:769	the body temperature	750:769	the body temperature	750:769	Rheological analysis demonstrated that the nanocomposites have good mechanical properties with gelation temperature close to the body temperature; hence, they can be easily administrated locally on wounded skins.					
31427021	8	23	theme	hydrogels	1235:1243	arg1	application					1197:1207	the potential application	1183:1207	the potential application of these novel injectable hydrogels	1183:1243	The obtained results highlight the potential application of these novel injectable hydrogels as wound dressing.					
31427021	7	24	theme	faster	1094:1099	arg1	closure					1107:1113	faster wound closure	1094:1113	faster wound closure	1094:1113	Interestingly, an in-vitro model of wound healing revealed that the nanocomposites allow faster wound closure and repair, compared to the control.					
31427021	2	25	theme	injectable	304:313	arg1	hydrogels					315:323	thermosensitive and injectable hydrogels	284:323	thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver	284:393	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	5	26	theme	gram-positive	890:902	arg1	bacteria					922:929	gram-positive and gram-negative bacteria	890:929	gram-positive and gram-negative bacteria	890:929	The samples exhibited antibacterial activity toward gram-positive and gram-negative bacteria.					
31427021	2	27	theme	care	444:447	arg1	material					449:456	a wound care material	436:456	a wound care material	436:456	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	6	28	theme	good	982:985	arg1	biocompatibility					987:1002	good biocompatibility	982:1002	good biocompatibility	982:1002	Cytotoxicity assay showed that the hydrogels have good biocompatibility.					
31427021	0	29	theme	wound	150:154	arg1	healing					156:162	potential wound healing	140:162	potential wound healing	140:162	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	3	30	theme	corn	572:575	arg1	extract					582:588	corn silk extract	572:588	corn silk extract	572:588	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	4	31	theme	Rheological	625:635	arg1	analysis					637:644	Rheological analysis	625:644	Rheological analysis	625:644	Rheological analysis demonstrated that the nanocomposites have good mechanical properties with gelation temperature close to the body temperature; hence, they can be easily administrated locally on wounded skins.					
31427021	2	32	theme	wound	438:442	arg1	material					449:456	a wound care material	436:456	a wound care material	436:456	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	0	33	theme	antibacterial	37:49	arg1	hydrogels					67:75	antibacterial thermosensitive hydrogels	37:75	antibacterial thermosensitive hydrogels	37:75	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	3	34	theme	solvent-free	604:615	arg1	medium					617:622	an organic solvent-free medium	593:622	an organic solvent-free medium	593:622	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	3	35	theme	silk	577:580	arg1	extract					582:588	corn silk extract	572:588	corn silk extract	572:588	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	7	36	theme	healing	1047:1053	arg1	model					1032:1036	an in-vitro model	1020:1036	an in-vitro model of wound healing	1020:1053	Interestingly, an in-vitro model of wound healing revealed that the nanocomposites allow faster wound closure and repair, compared to the control.					
31427021	8	37	theme	wound	1248:1252	arg1	dressing					1254:1261	wound dressing	1248:1261	wound dressing	1248:1261	The obtained results highlight the potential application of these novel injectable hydrogels as wound dressing.					
31427021	1	38	dep	clinical	235:242	arg1	closure					176:182	The wounds closure	165:182	The wounds closure after physical injury or surgery	165:215	The wounds closure after physical injury or surgery is of significant clinical and research importance.					
31427021	1	38	dep	clinical	235:242	arg1	importance					257:266	importance	257:266	importance	257:266	The wounds closure after physical injury or surgery is of significant clinical and research importance.					
31427021	4	39	theme	wounded	823:829	arg1	skins					831:835	wounded skins	823:835	wounded skins	823:835	Rheological analysis demonstrated that the nanocomposites have good mechanical properties with gelation temperature close to the body temperature; hence, they can be easily administrated locally on wounded skins.					
31427021	7	40	theme	wound	1101:1105	arg1	closure					1107:1113	faster wound closure	1094:1113	faster wound closure	1094:1113	Interestingly, an in-vitro model of wound healing revealed that the nanocomposites allow faster wound closure and repair, compared to the control.					
31427021	0	41	theme	potential	140:148	arg1	healing					156:162	potential wound healing	140:162	potential wound healing	140:162	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	7	42	theme	wound	1041:1045	arg1	healing					1047:1053	wound healing	1041:1053	wound healing	1041:1053	Interestingly, an in-vitro model of wound healing revealed that the nanocomposites allow faster wound closure and repair, compared to the control.					
31427021	3	43	theme	organic	596:602	arg1	medium					617:622	an organic solvent-free medium	593:622	an organic solvent-free medium	593:622	Silver nanoparticles (Ag NPs) were biosynthesized by a microwave-assisted green technique using corn silk extract in an organic solvent-free medium.					
31427021	7	44	theme	in-vitro	1023:1030	arg1	model					1032:1036	an in-vitro model	1020:1036	an in-vitro model of wound healing	1020:1053	Interestingly, an in-vitro model of wound healing revealed that the nanocomposites allow faster wound closure and repair, compared to the control.					
31427021	2	45	theme	hyaluronic	334:343	arg1	HA					351:352	HA	351:352	HA	351:352	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	2	45	theme	hyaluronic	334:343	arg1	acid					345:348	hyaluronic acid	334:348	hyaluronic acid (HA)	334:353	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	0	46	theme	corn	86:89	arg1	nanosilver					125:134	nanosilver	125:134	nanosilver for potential wound healing	125:162	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	0	46	theme	corn	86:89	arg1	extract					96:102	corn silk extract	86:102	corn silk extract	86:102	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	0	46	theme	corn	86:89	arg1	acid					116:119	hyaluronic acid	105:119	hyaluronic acid	105:119	Biosynthesis and characterization of antibacterial thermosensitive hydrogels based on corn silk extract, hyaluronic acid and nanosilver for potential wound healing.					
31427021	2	47	theme	silk	361:364	arg1	CSE					375:377	CSE	375:377	CSE	375:377	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	2	47	theme	silk	361:364	arg1	extract					366:372	corn silk extract	356:372	corn silk extract (CSE)	356:378	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	8	48	theme	obtained	1156:1163	arg1	results					1165:1171	The obtained results	1152:1171	The obtained results	1152:1171	The obtained results highlight the potential application of these novel injectable hydrogels as wound dressing.					
31427021	6	49	contain	have	977:980	arg1	hydrogels					967:975	the hydrogels	963:975	the hydrogels	963:975	Cytotoxicity assay showed that the hydrogels have good biocompatibility.					
31427021	6	49	contain	have	977:980	arg2	biocompatibility					987:1002	good biocompatibility	982:1002	good biocompatibility	982:1002	Cytotoxicity assay showed that the hydrogels have good biocompatibility.					
31427021	2	50	theme	corn	356:359	arg1	CSE					375:377	CSE	375:377	CSE	375:377	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
31427021	2	50	theme	corn	356:359	arg1	extract					366:372	corn silk extract	356:372	corn silk extract (CSE)	356:378	In this study, thermosensitive and injectable hydrogels based on hyaluronic acid (HA), corn silk extract (CSE) and nanosilver were prepared and their potential use as a wound care material was investigated.					
29655312	7	0	theme	porous	1591:1596	arg1	scaffold					1598:1605	the pure CS porous scaffold or UHANWs/CS porous scaffold	1550:1605	scaffold	1598:1605	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	4	1	theme	%	930:930	arg1	Zn-UHANWs					932:940	80 wt % Zn-UHANWs	924:940	80 wt % Zn-UHANWs	924:940	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	1	2	theme	great	210:214	arg1	potential					216:224	a great potential	208:224	a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone	208:408	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	7	3	theme	porous	1562:1567	arg1	scaffold					1569:1576	the pure CS porous scaffold or UHANWs/CS porous scaffold	1550:1605	scaffold	1569:1576	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	6	4	theme	sustainable	1281:1291	arg1	release					1293:1299	the sustainable release	1277:1299	the sustainable release of Zn, Ca, and P elements in aqueous solution	1277:1345	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	3	5	theme	rough	655:659	arg1	surface					661:667	a hierarchical rough surface	640:667	a hierarchical rough surface	640:667	The highly flexible Zn-UHANWs exhibit a hierarchical rough surface and enhanced specific surface area as compared with ultralong hydroxyapatite nanowires (UHANWs).					
29655312	7	6	dep	in	1507:1508	arg1	vivo					1510:1513	vivo	1510:1513	vivo	1510:1513	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	7	7	dep	derived	1461:1467	arg1	marrow					1454:1459	rat bone marrow	1445:1459	rat bone marrow	1445:1459	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	6	8	contain	has	1244:1246	arg2	biodegradability					1255:1270	a good biodegradability	1248:1270	a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution	1248:1345	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	6	8	contain	has	1244:1246	arg1	scaffold					1235:1242	the Zn-UHANWs/CS porous scaffold	1211:1242	the Zn-UHANWs/CS porous scaffold	1211:1242	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	0	9	theme	Defect	160:165	arg1	Repair					167:172	Bone Defect Repair	155:172	Bone Defect Repair	155:172	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	3	10	theme	specific	682:689	arg1	area					699:702	enhanced specific surface area	673:702	enhanced specific surface area	673:702	The highly flexible Zn-UHANWs exhibit a hierarchical rough surface and enhanced specific surface area as compared with ultralong hydroxyapatite nanowires (UHANWs).					
29655312	4	11	dep	composite	893:901	arg1	Zn-UHANWs/chitosan					854:871	biomimetic Zn-UHANWs/chitosan	843:871	biomimetic Zn-UHANWs/chitosan (CS)	843:876	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	4	11	dep	composite	893:901	arg1	CS					874:875	CS	874:875	CS	874:875	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	6	12	with	biodegradability	1255:1270	arg1	release					1293:1299	the sustainable release	1277:1299	the sustainable release of Zn, Ca, and P elements in aqueous solution	1277:1345	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	7	13	theme	cells	1486:1490	arg1	differentiation					1426:1440	the osteogenic differentiation	1411:1440	the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells	1411:1490	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	7	14	theme	mesenchymal	1469:1479	arg1	cells					1486:1490	rat bone marrow derived mesenchymal stem cells	1445:1490	rat bone marrow derived mesenchymal stem cells	1445:1490	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	6	15	theme	P	1316:1316	arg1	elements					1318:1325	P elements	1316:1325	P elements	1316:1325	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	5	16	theme	water	1172:1176	arg1	capacity					1188:1195	excellent water retention capacity	1162:1195	excellent water retention capacity	1162:1195	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	1	17	from	potential	216:224	arg1	applications					240:251	biomedical applications	229:251	biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone	229:408	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	0	18	theme	Zinc-Containing	82:96	arg1	Nanoparticles					98:110	Zinc-Containing Nanoparticles	82:110	Zinc-Containing Nanoparticles	82:110	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	1	19	theme	mineralized	365:375	arg1	fibrils					386:392	mineralized collagen fibrils	365:392	mineralized collagen fibrils of natural bone	365:408	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	1	20	theme	Hydroxyapatite	175:188	arg1	nanowires					190:198	Hydroxyapatite nanowires	175:198	Hydroxyapatite nanowires	175:198	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	4	21	theme	potential	782:790	arg1	application					792:802	the potential application	778:802	the potential application of Zn-UHANWs in bone regeneration	778:836	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	1	22	theme	natural	397:403	arg1	bone					405:408	natural bone	397:408	natural bone	397:408	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	6	23	theme	Zn	1304:1305	arg1	release					1293:1299	the sustainable release	1277:1299	the sustainable release of Zn, Ca, and P elements in aqueous solution	1277:1345	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	5	24	theme	porous	1076:1081	arg1	scaffold					1083:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold	1037:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold	1037:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	2	25	theme	solvothermal	581:592	arg1	method					594:599	a one-step solvothermal method	570:599	a one-step solvothermal method	570:599	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	6	26	from	release	1293:1299	arg1	solution					1338:1345	aqueous solution	1330:1345	aqueous solution	1330:1345	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	2	27	theme	zinc-containing	425:439	arg1	Zn-UHANWs					500:508	Zn-UHANWs	500:508	Zn-UHANWs	500:508	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	2	27	theme	zinc-containing	425:439	arg1	nanowires					489:497	zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires	425:497	zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure	425:543	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	7	28	theme	bone	1449:1452	arg1	marrow					1454:1459	rat bone marrow	1445:1459	rat bone marrow	1445:1459	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	8	29	theme	Zn-UHANWs/CS	1637:1648	arg1	scaffold					1657:1664	Zn-UHANWs/CS porous scaffold	1637:1664	Zn-UHANWs/CS porous scaffold	1637:1664	Thus, both the Zn-UHANWs and Zn-UHANWs/CS porous scaffold developed in this work are promising for application in bone defect repair.					
29655312	0	30	theme	Porous	0:5	arg1	Nanocomposite					7:19	Porous Nanocomposite	0:19	Porous Nanocomposite Comprising Ultralong Hydroxyapatite	0:55	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	1	31	theme	mechanical	324:333	arg1	properties					335:344	excellent mechanical properties	314:344	excellent mechanical properties	314:344	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	5	32	theme	Zn-UHANWs/CS	1053:1064	arg1	scaffold					1083:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold	1037:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold	1037:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	7	33	theme	osteogenic	1415:1424	arg1	differentiation					1426:1440	the osteogenic differentiation	1411:1440	the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells	1411:1490	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	4	34	theme	porous	903:908	arg1	scaffold					910:917	the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold	839:917	the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs	839:940	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	2	35	theme	ultralong	464:472	arg1	Zn-UHANWs					500:508	Zn-UHANWs	500:508	Zn-UHANWs	500:508	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	2	35	theme	ultralong	464:472	arg1	nanowires					489:497	zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires	425:497	zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure	425:543	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	5	36	theme	porous	1140:1145	arg1	structure					1147:1155	highly porous structure	1133:1155	highly porous structure	1133:1155	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	2	37	theme	hierarchical	518:529	arg1	nanostructure					531:543	a hierarchical nanostructure	516:543	a hierarchical nanostructure	516:543	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	7	38	theme	CS	1559:1560	arg1	scaffold					1569:1576	the pure CS porous scaffold or UHANWs/CS porous scaffold	1550:1605	scaffold	1569:1576	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	7	39	theme	derived	1461:1467	arg1	cells					1486:1490	rat bone marrow derived mesenchymal stem cells	1445:1490	rat bone marrow derived mesenchymal stem cells	1445:1490	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	5	40	theme	mechanical	1110:1119	arg1	properties					1121:1130	enhanced mechanical properties	1101:1130	enhanced mechanical properties	1101:1130	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	7	41	theme	Zn-UHANWs/CS	1370:1381	arg1	scaffold					1390:1397	the Zn-UHANWs/CS porous scaffold	1366:1397	the Zn-UHANWs/CS porous scaffold	1366:1397	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	4	42	from	application	792:802	arg1	regeneration					825:836	bone regeneration	820:836	bone regeneration	820:836	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	1	43	theme	specific	273:280	arg1	area					290:293	their high specific surface area	262:293	their high specific surface area	262:293	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	6	44	theme	Zn-UHANWs/CS	1215:1226	arg1	scaffold					1235:1242	the Zn-UHANWs/CS porous scaffold	1211:1242	the Zn-UHANWs/CS porous scaffold	1211:1242	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	8	45	theme	bone	1722:1725	arg1	repair					1734:1739	bone defect repair	1722:1739	bone defect repair	1722:1739	Thus, both the Zn-UHANWs and Zn-UHANWs/CS porous scaffold developed in this work are promising for application in bone defect repair.					
29655312	7	46	theme	UHANWs/CS	1581:1589	arg1	scaffold					1598:1605	the pure CS porous scaffold or UHANWs/CS porous scaffold	1550:1605	scaffold	1598:1605	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	3	47	theme	flexible	613:620	arg1	Zn-UHANWs					622:630	The highly flexible Zn-UHANWs	602:630	The highly flexible Zn-UHANWs	602:630	The highly flexible Zn-UHANWs exhibit a hierarchical rough surface and enhanced specific surface area as compared with ultralong hydroxyapatite nanowires (UHANWs).					
29655312	1	48	theme	high	296:299	arg1	flexibility					301:311	high flexibility	296:311	high flexibility	296:311	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	7	49	theme	pure	1554:1557	arg1	scaffold					1569:1576	the pure CS porous scaffold or UHANWs/CS porous scaffold	1550:1605	scaffold	1569:1576	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	6	50	theme	good	1250:1253	arg1	biodegradability					1255:1270	a good biodegradability	1248:1270	a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution	1248:1345	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	2	51	with	nanowires	489:497	arg1	nanostructure					531:543	a hierarchical nanostructure	516:543	a hierarchical nanostructure	516:543	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	4	52	theme	80 wt 	924:929	arg1	%					930:930	%	930:930	%	930:930	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	3	53	theme	hierarchical	642:653	arg1	surface					661:667	a hierarchical rough surface	640:667	a hierarchical rough surface	640:667	The highly flexible Zn-UHANWs exhibit a hierarchical rough surface and enhanced specific surface area as compared with ultralong hydroxyapatite nanowires (UHANWs).					
29655312	0	54	theme	Bone	155:158	arg1	Repair					167:172	Bone Defect Repair	155:172	Bone Defect Repair	155:172	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	1	55	theme	biomedical	229:238	arg1	applications					240:251	biomedical applications	229:251	biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone	229:408	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	7	56	theme	bone	1515:1518	arg1	regeneration					1520:1531	in vivo bone regeneration	1507:1531	in vivo bone regeneration	1507:1531	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	3	57	theme	enhanced	673:680	arg1	area					699:702	enhanced specific surface area	673:702	enhanced specific surface area	673:702	The highly flexible Zn-UHANWs exhibit a hierarchical rough surface and enhanced specific surface area as compared with ultralong hydroxyapatite nanowires (UHANWs).					
29655312	4	58	theme	freeze-drying	1014:1026	arg1	process					1028:1034	the freeze-drying process	1010:1034	the freeze-drying process	1010:1034	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	6	59	theme	aqueous	1330:1336	arg1	solution					1338:1345	aqueous solution	1330:1345	aqueous solution	1330:1345	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	0	60	from	Application	140:150	arg1	Repair					167:172	Bone Defect Repair	155:172	Bone Defect Repair	155:172	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	6	61	theme	elements	1318:1325	arg1	release					1293:1299	the sustainable release	1277:1299	the sustainable release of Zn, Ca, and P elements in aqueous solution	1277:1345	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	5	62	theme	excellent	1162:1170	arg1	capacity					1188:1195	excellent water retention capacity	1162:1195	excellent water retention capacity	1162:1195	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	4	63	theme	chitosan	991:998	arg1	matrix					1000:1005	the chitosan matrix	987:1005	the chitosan matrix by the freeze-drying process	987:1034	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	0	64	from	Synthesis	126:134	arg1	Repair					167:172	Bone Defect Repair	155:172	Bone Defect Repair	155:172	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	7	65	theme	stem	1481:1484	arg1	cells					1486:1490	rat bone marrow derived mesenchymal stem cells	1445:1490	rat bone marrow derived mesenchymal stem cells	1445:1490	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	4	66	theme	Zn-UHANWs	807:815	arg1	application					792:802	the potential application	778:802	the potential application of Zn-UHANWs in bone regeneration	778:836	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	4	67	with	scaffold	910:917	arg1	Zn-UHANWs					932:940	80 wt % Zn-UHANWs	924:940	80 wt % Zn-UHANWs	924:940	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	5	68	theme	retention	1178:1186	arg1	capacity					1188:1195	excellent water retention capacity	1162:1195	excellent water retention capacity	1162:1195	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	3	69	theme	surface	691:697	arg1	area					699:702	enhanced specific surface area	673:702	enhanced specific surface area	673:702	The highly flexible Zn-UHANWs exhibit a hierarchical rough surface and enhanced specific surface area as compared with ultralong hydroxyapatite nanowires (UHANWs).					
29655312	6	70	theme	Ca	1308:1309	arg1	release					1293:1299	the sustainable release	1277:1299	the sustainable release of Zn, Ca, and P elements in aqueous solution	1277:1345	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	1	71	theme	collagen	377:384	arg1	fibrils					386:392	mineralized collagen fibrils	365:392	mineralized collagen fibrils of natural bone	365:408	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	5	72	theme	composite	1066:1074	arg1	scaffold					1083:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold	1037:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold	1037:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	4	73	theme	biomimetic	843:852	arg1	Zn-UHANWs/chitosan					854:871	biomimetic Zn-UHANWs/chitosan	843:871	biomimetic Zn-UHANWs/chitosan (CS)	843:876	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	4	73	theme	biomimetic	843:852	arg1	CS					874:875	CS	874:875	CS	874:875	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	1	74	theme	bone	405:408	arg1	fibrils					386:392	mineralized collagen fibrils	365:392	mineralized collagen fibrils of natural bone	365:408	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	7	75	theme	rat	1445:1447	arg1	marrow					1454:1459	rat bone marrow	1445:1459	rat bone marrow	1445:1459	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	2	76	theme	one-step	572:579	arg1	method					594:599	a one-step solvothermal method	570:599	a one-step solvothermal method	570:599	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	8	77	theme	porous	1650:1655	arg1	scaffold					1657:1664	Zn-UHANWs/CS porous scaffold	1637:1664	Zn-UHANWs/CS porous scaffold	1637:1664	Thus, both the Zn-UHANWs and Zn-UHANWs/CS porous scaffold developed in this work are promising for application in bone defect repair.					
29655312	7	78	link	derived	1461:1467	arg1	cells					1486:1490	rat bone marrow derived mesenchymal stem cells	1445:1490	rat bone marrow derived mesenchymal stem cells	1445:1490	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	4	79	theme	bone	820:823	arg1	regeneration					825:836	bone regeneration	820:836	bone regeneration	820:836	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	0	80	theme	Ultralong	32:40	arg1	Hydroxyapatite					42:55	Ultralong Hydroxyapatite	32:55	Ultralong Hydroxyapatite	32:55	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	1	81	theme	excellent	314:322	arg1	properties					335:344	excellent mechanical properties	314:344	excellent mechanical properties	314:344	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	5	82	theme	as-prepared	1041:1051	arg1	scaffold					1083:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold	1037:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold	1037:1090	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	3	83	theme	hydroxyapatite	731:744	arg1	UHANWs					757:762	UHANWs	757:762	UHANWs	757:762	The highly flexible Zn-UHANWs exhibit a hierarchical rough surface and enhanced specific surface area as compared with ultralong hydroxyapatite nanowires (UHANWs).					
29655312	3	83	theme	hydroxyapatite	731:744	arg1	nanowires					746:754	hydroxyapatite nanowires	731:754	hydroxyapatite nanowires (UHANWs)	731:763	The highly flexible Zn-UHANWs exhibit a hierarchical rough surface and enhanced specific surface area as compared with ultralong hydroxyapatite nanowires (UHANWs).					
29655312	2	84	theme	hydroxyapatite	474:487	arg1	Zn-UHANWs					500:508	Zn-UHANWs	500:508	Zn-UHANWs	500:508	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	2	84	theme	hydroxyapatite	474:487	arg1	nanowires					489:497	zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires	425:497	zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure	425:543	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	4	85	theme	composite	893:901	arg1	scaffold					910:917	the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold	839:917	the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs	839:940	To evaluate the potential application of Zn-UHANWs in bone regeneration, the biomimetic Zn-UHANWs/chitosan (CS) (Zn-UHANWs/CS) composite porous scaffold with 80 wt % Zn-UHANWs was prepared by incorporating Zn-UHANWs into the chitosan matrix by the freeze-drying process.					
29655312	2	86	theme	nanoparticle-decorated	441:462	arg1	Zn-UHANWs					500:508	Zn-UHANWs	500:508	Zn-UHANWs	500:508	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	2	86	theme	nanoparticle-decorated	441:462	arg1	nanowires					489:497	zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires	425:497	zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure	425:543	In this work, zinc-containing nanoparticle-decorated ultralong hydroxyapatite nanowires (Zn-UHANWs) with a hierarchical nanostructure have been synthesized by a one-step solvothermal method.					
29655312	0	87	dep	Nanowires	57:65	arg1	Synthesis					126:134	Synthesis	126:134	Synthesis	126:134	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	0	87	dep	Nanowires	57:65	arg1	Decorated					67:75	Decorated	67:75	Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan	57:123	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	0	87	dep	Nanowires	57:65	arg1	Application					140:150	Application	140:150	Application	140:150	Porous Nanocomposite Comprising Ultralong Hydroxyapatite Nanowires Decorated with Zinc-Containing Nanoparticles and Chitosan: Synthesis and Application in Bone Defect Repair.					
29655312	7	88	theme	porous	1383:1388	arg1	scaffold					1390:1397	the Zn-UHANWs/CS porous scaffold	1366:1397	the Zn-UHANWs/CS porous scaffold	1366:1397	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	8	89	from	application	1707:1717	arg1	repair					1734:1739	bone defect repair	1722:1739	bone defect repair	1722:1739	Thus, both the Zn-UHANWs and Zn-UHANWs/CS porous scaffold developed in this work are promising for application in bone defect repair.					
29655312	5	90	theme	enhanced	1101:1108	arg1	properties					1121:1130	enhanced mechanical properties	1101:1130	enhanced mechanical properties	1101:1130	The as-prepared Zn-UHANWs/CS composite porous scaffold exhibits enhanced mechanical properties, highly porous structure, and excellent water retention capacity.					
29655312	7	91	theme	in	1507:1508	arg1	regeneration					1520:1531	in vivo bone regeneration	1507:1531	in vivo bone regeneration	1507:1531	More importantly, the Zn-UHANWs/CS porous scaffold can promote the osteogenic differentiation of rat bone marrow derived mesenchymal stem cells and facilitate in vivo bone regeneration as compared with the pure CS porous scaffold or UHANWs/CS porous scaffold.					
29655312	1	92	theme	high	268:271	arg1	area					290:293	their high specific surface area	262:293	their high specific surface area	262:293	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
29655312	6	93	theme	porous	1228:1233	arg1	scaffold					1235:1242	the Zn-UHANWs/CS porous scaffold	1211:1242	the Zn-UHANWs/CS porous scaffold	1211:1242	In addition, the Zn-UHANWs/CS porous scaffold has a good biodegradability with the sustainable release of Zn, Ca, and P elements in aqueous solution.					
29655312	8	94	theme	defect	1727:1732	arg1	repair					1734:1739	bone defect repair	1722:1739	bone defect repair	1722:1739	Thus, both the Zn-UHANWs and Zn-UHANWs/CS porous scaffold developed in this work are promising for application in bone defect repair.					
29655312	8	95	dep	Zn-UHANWs	1623:1631	arg1	the					1619:1621	the	1619:1621	the	1619:1621	Thus, both the Zn-UHANWs and Zn-UHANWs/CS porous scaffold developed in this work are promising for application in bone defect repair.					
29655312	1	96	theme	surface	282:288	arg1	area					290:293	their high specific surface area	262:293	their high specific surface area	262:293	Hydroxyapatite nanowires exhibit a great potential in biomedical applications owing to their high specific surface area, high flexibility, excellent mechanical properties, and similarity to mineralized collagen fibrils of natural bone.					
31473409	0	0	theme	chitosan	86:93	arg1	derivatives					95:105	O-carboxymethyl chitosan derivatives	70:105	O-carboxymethyl chitosan derivatives	70:105	A novel manganese chelated macromolecular MRI contrast agent based on O-carboxymethyl chitosan derivatives.					
31473409	3	1	theme	aqueous	645:651	arg1	solution					653:660	aqueous solution	645:660	aqueous solution	645:660	The relaxivity of CMCS-(Mn-DTPA)n is approximately 3.5 and 5.5 times higher than that of Gd-DTPA and Mn-DPDP in aqueous solution, respectively.					
31473409	1	2	theme	contrast	165:172	arg1	agents					174:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	1	3	theme	potential	249:257	arg1	risk					266:269	the potential safety risk	245:269	the potential safety risk under high administration dose or repeat dosing	245:317	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	0	4	theme	O-carboxymethyl	70:84	arg1	derivatives					95:105	O-carboxymethyl chitosan derivatives	70:105	O-carboxymethyl chitosan derivatives	70:105	A novel manganese chelated macromolecular MRI contrast agent based on O-carboxymethyl chitosan derivatives.					
31473409	1	5	theme	safety	259:264	arg1	risk					266:269	the potential safety risk	245:269	the potential safety risk under high administration dose or repeat dosing	245:317	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	7	6	theme	CMCS-	1199:1203	arg1	n					1213:1213	CMCS-(Mn-DTPA)n	1199:1213	CMCS-(Mn-DTPA)n	1199:1213	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	4	7	theme	0.03 mM	794:800	arg1	n					827:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	4	8	theme	rats	749:752	arg1	intensity					692:700	The MRI signal intensity	677:700	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats	677:752	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	4	9	theme	signal	685:690	arg1	intensity					692:700	The MRI signal intensity	677:700	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats	677:752	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	7	10	theme	excellent	1276:1284	arg1	relaxivity					1286:1295	its excellent relaxivity	1272:1295	its excellent relaxivity	1272:1295	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	7	11	theme	due	1265:1267	arg1	agent					1259:1263	a potentially novel MRI contrast agent	1226:1263	a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility	1226:1352	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	2	12	theme	present	350:356	arg1	study					358:362	the present study	346:362	the present study	346:362	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	2	13	theme	Mn-DTPA	493:499	arg1	chitosan					470:477	O-carboxymethyl chitosan	454:477	O-carboxymethyl chitosan (CMCS)	454:484	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	2	13	theme	Mn-DTPA	493:499	arg1	n					501:501	CMCS-(Mn-DTPA)n	487:501	CMCS-(Mn-DTPA)n	487:501	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	6	14	theme	in	1039:1040	arg1	studies					1047:1053	in vivo studies	1039:1053	in vivo studies	1039:1053	Based on the results from in vivo studies, manganese (Mn) is completely excreted from SD rats within ten days after administration and does not exert a pathological effect on the liver.					
31473409	2	15	theme	magnetic	396:403	arg1	resonance					405:413	a biocompatible macromolecular magnetic resonance	365:413	a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n	365:501	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	4	16	theme	long	846:849	arg1	window					869:874	a long effective imaging window	844:874	a long effective imaging window	844:874	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	4	17	theme	Dawley	737:742	arg1	rats					749:752	Sprague Dawley (SD) rats	729:752	Sprague Dawley (SD) rats	729:752	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	4	18	from	intensity	692:700	arg1	liver					720:724	liver	720:724	liver	720:724	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	4	18	from	intensity	692:700	arg1	kidney					709:714	kidney	709:714	kidney	709:714	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	7	19	theme	contrast	1250:1257	arg1	agent					1259:1263	a potentially novel MRI contrast agent	1226:1263	a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility	1226:1352	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	6	20	from	studies	1047:1053	arg1	results					1026:1032	the results	1022:1032	the results from in vivo studies	1022:1053	Based on the results from in vivo studies, manganese (Mn) is completely excreted from SD rats within ten days after administration and does not exert a pathological effect on the liver.					
31473409	2	21	theme	macromolecular	381:394	arg1	resonance					405:413	a biocompatible macromolecular magnetic resonance	365:413	a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n	365:501	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	0	22	theme	novel	2:6	arg1	manganese					8:16	A novel manganese	0:16	A novel manganese	0:16	A novel manganese chelated macromolecular MRI contrast agent based on O-carboxymethyl chitosan derivatives.					
31473409	5	23	theme	necessary	986:994	arg1	dose					981:984	the dose	977:984	the dose necessary for MRI imaging	977:1010	According to in vitro studies, CMCS-(Mn-DTPA)n exhibits good cellular and blood biocompatibility at the dose necessary for MRI imaging.					
31473409	6	24	theme	SD	1099:1100	arg1	rats					1102:1105	SD rats	1099:1105	SD rats	1099:1105	Based on the results from in vivo studies, manganese (Mn) is completely excreted from SD rats within ten days after administration and does not exert a pathological effect on the liver.					
31473409	4	25	theme	MRI	681:683	arg1	intensity					692:700	The MRI signal intensity	677:700	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats	677:752	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	4	26	dep	kidney	709:714	arg1	the					705:707	the	705:707	the	705:707	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	2	27	theme	biocompatible	367:379	arg1	resonance					405:413	a biocompatible macromolecular magnetic resonance	365:413	a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n	365:501	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	4	28	dep	increased	771:779	arg1	accompanied					829:839	accompanied	829:839	accompanied by a long effective imaging window	829:874	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	1	29	theme	high	277:280	arg1	dose					297:300	high administration dose	277:300	high administration dose	277:300	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	7	30	theme	imaging	1313:1319	arg1	window					1321:1326	long effective imaging window	1298:1326	long effective imaging window	1298:1326	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	7	31	theme	novel	1240:1244	arg1	agent					1259:1263	a potentially novel MRI contrast agent	1226:1263	a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility	1226:1352	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	2	32	theme	contrast	429:436	arg1	agents					438:443	a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents	365:443	a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n	365:501	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	1	33	theme	administration	282:295	arg1	dose					297:300	high administration dose	277:300	high administration dose	277:300	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	7	34	theme	Mn-DTPA	1205:1211	arg1	n					1213:1213	CMCS-(Mn-DTPA)n	1199:1213	CMCS-(Mn-DTPA)n	1199:1213	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	6	35	dep	in	1039:1040	arg1	vivo					1042:1045	vivo	1042:1045	vivo	1042:1045	Based on the results from in vivo studies, manganese (Mn) is completely excreted from SD rats within ten days after administration and does not exert a pathological effect on the liver.					
31473409	5	36	theme	MRI	1000:1002	arg1	imaging					1004:1010	MRI imaging	1000:1010	MRI imaging	1000:1010	According to in vitro studies, CMCS-(Mn-DTPA)n exhibits good cellular and blood biocompatibility at the dose necessary for MRI imaging.					
31473409	4	37	theme	Mn-DTPA	819:825	arg1	n					827:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	1	38	theme	used	118:121	arg1	agents					174:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	0	39	theme	MRI	42:44	arg1	agent					55:59	macromolecular MRI contrast agent	27:59	macromolecular MRI contrast agent	27:59	A novel manganese chelated macromolecular MRI contrast agent based on O-carboxymethyl chitosan derivatives.					
31473409	6	40	from	effect	1178:1183	arg1	liver					1192:1196	the liver	1188:1196	the liver	1188:1196	Based on the results from in vivo studies, manganese (Mn) is completely excreted from SD rats within ten days after administration and does not exert a pathological effect on the liver.					
31473409	6	41	theme	pathological	1165:1176	arg1	effect					1178:1183	a pathological effect	1163:1183	a pathological effect on the liver	1163:1196	Based on the results from in vivo studies, manganese (Mn) is completely excreted from SD rats within ten days after administration and does not exert a pathological effect on the liver.					
31473409	2	42	theme	imaging	415:421	arg1	agents					438:443	a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents	365:443	a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n	365:501	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	0	43	theme	macromolecular	27:40	arg1	agent					55:59	macromolecular MRI contrast agent	27:59	macromolecular MRI contrast agent	27:59	A novel manganese chelated macromolecular MRI contrast agent based on O-carboxymethyl chitosan derivatives.					
31473409	1	44	theme	repeat	305:310	arg1	dosing					312:317	repeat dosing	305:317	repeat dosing	305:317	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	4	45	theme	n	827:827	arg1	dose					786:789	a dose	784:789	a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	784:827	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	5	46	theme	blood	951:955	arg1	biocompatibility					957:972	blood biocompatibility	951:972	blood biocompatibility	951:972	According to in vitro studies, CMCS-(Mn-DTPA)n exhibits good cellular and blood biocompatibility at the dose necessary for MRI imaging.					
31473409	5	47	theme	CMCS-	908:912	arg1	n					922:922	CMCS-(Mn-DTPA)n	908:922	CMCS-(Mn-DTPA)n	908:922	According to in vitro studies, CMCS-(Mn-DTPA)n exhibits good cellular and blood biocompatibility at the dose necessary for MRI imaging.					
31473409	1	48	theme	long-term	219:227	arg1	monitoring					229:238	the long-term monitoring	215:238	the long-term monitoring	215:238	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	2	49	theme	O-carboxymethyl	454:468	arg1	CMCS					480:483	CMCS	480:483	CMCS	480:483	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	2	49	theme	O-carboxymethyl	454:468	arg1	chitosan					470:477	O-carboxymethyl chitosan	454:477	O-carboxymethyl chitosan (CMCS)	454:484	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	2	49	theme	O-carboxymethyl	454:468	arg1	n					501:501	CMCS-(Mn-DTPA)n	487:501	CMCS-(Mn-DTPA)n	487:501	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	4	50	theme	b.w.	808:811	arg1	n					827:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	3	51	theme	CMCS-	551:555	arg1	Mn-DTPA					557:563	Mn-DTPA	557:563	Mn-DTPA	557:563	The relaxivity of CMCS-(Mn-DTPA)n is approximately 3.5 and 5.5 times higher than that of Gd-DTPA and Mn-DPDP in aqueous solution, respectively.					
31473409	3	51	theme	CMCS-	551:555	arg1	n					565:565	CMCS-(Mn-DTPA)n	551:565	CMCS-(Mn-DTPA)n	551:565	The relaxivity of CMCS-(Mn-DTPA)n is approximately 3.5 and 5.5 times higher than that of Gd-DTPA and Mn-DPDP in aqueous solution, respectively.					
31473409	1	52	theme	Gd-based	123:130	arg1	agents					174:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	0	53	theme	contrast	46:53	arg1	agent					55:59	macromolecular MRI contrast agent	27:59	macromolecular MRI contrast agent	27:59	A novel manganese chelated macromolecular MRI contrast agent based on O-carboxymethyl chitosan derivatives.					
31473409	4	54	theme	Mn/kg	802:806	arg1	n					827:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	7	55	theme	good	1332:1335	arg1	biocompatibility					1337:1352	good biocompatibility	1332:1352	good biocompatibility	1332:1352	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	5	56	theme	Mn-DTPA	914:920	arg1	n					922:922	CMCS-(Mn-DTPA)n	908:922	CMCS-(Mn-DTPA)n	908:922	According to in vitro studies, CMCS-(Mn-DTPA)n exhibits good cellular and blood biocompatibility at the dose necessary for MRI imaging.					
31473409	4	57	theme	effective	851:859	arg1	window					869:874	a long effective imaging window	844:874	a long effective imaging window	844:874	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	7	58	theme	MRI	1246:1248	arg1	agent					1259:1263	a potentially novel MRI contrast agent	1226:1263	a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility	1226:1352	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	1	59	theme	Mn-based	136:143	arg1	agents					174:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	5	60	dep	in	890:891	arg1	vitro					893:897	vitro	893:897	vitro	893:897	According to in vitro studies, CMCS-(Mn-DTPA)n exhibits good cellular and blood biocompatibility at the dose necessary for MRI imaging.					
31473409	4	61	theme	CMCS-	813:817	arg1	n					827:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n	794:827	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	5	62	theme	in	890:891	arg1	studies					899:905	in vitro studies	890:905	in vitro studies	890:905	According to in vitro studies, CMCS-(Mn-DTPA)n exhibits good cellular and blood biocompatibility at the dose necessary for MRI imaging.					
31473409	1	63	theme	small	145:149	arg1	agents					174:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	2	64	theme	CMCS-	487:491	arg1	chitosan					470:477	O-carboxymethyl chitosan	454:477	O-carboxymethyl chitosan (CMCS)	454:484	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	2	64	theme	CMCS-	487:491	arg1	n					501:501	CMCS-(Mn-DTPA)n	487:501	CMCS-(Mn-DTPA)n	487:501	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	2	65	theme	resonance	405:413	arg1	imaging					415:421	a biocompatible macromolecular magnetic resonance imaging	365:421	a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n	365:501	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	2	65	theme	resonance	405:413	arg1	MRI					424:426	MRI	424:426	MRI	424:426	In the present study, a biocompatible macromolecular magnetic resonance imaging (MRI) contrast agents based on O-carboxymethyl chitosan (CMCS), CMCS-(Mn-DTPA)n was designed and synthesized.					
31473409	3	66	theme	n	565:565	arg1	higher					602:607	higher	602:607	higher	602:607	The relaxivity of CMCS-(Mn-DTPA)n is approximately 3.5 and 5.5 times higher than that of Gd-DTPA and Mn-DPDP in aqueous solution, respectively.					
31473409	3	66	theme	n	565:565	arg1	relaxivity					537:546	The relaxivity	533:546	The relaxivity of CMCS-(Mn-DTPA)n	533:565	The relaxivity of CMCS-(Mn-DTPA)n is approximately 3.5 and 5.5 times higher than that of Gd-DTPA and Mn-DPDP in aqueous solution, respectively.					
31473409	1	67	theme	molecular	151:159	arg1	agents					174:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
31473409	7	68	theme	long	1298:1301	arg1	window					1321:1326	long effective imaging window	1298:1326	long effective imaging window	1298:1326	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	4	69	theme	imaging	861:867	arg1	window					869:874	a long effective imaging window	844:874	a long effective imaging window	844:874	The MRI signal intensity in the kidney and liver of Sprague Dawley (SD) rats is significantly increased at a dose of 0.03 mM Mn/kg b.w. CMCS-(Mn-DTPA)n accompanied by a long effective imaging window.					
31473409	7	70	theme	effective	1303:1311	arg1	window					1321:1326	long effective imaging window	1298:1326	long effective imaging window	1298:1326	CMCS-(Mn-DTPA)n represents a potentially novel MRI contrast agent due to its excellent relaxivity, long effective imaging window and good biocompatibility.					
31473409	1	71	theme	MRI	161:163	arg1	agents					174:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents	108:179	Currently used Gd-based and Mn-based small molecular MRI contrast agents fail to meet the requirements for the long-term monitoring, and the potential safety risk under high administration dose or repeat dosing needs to be considered.					
30715624	8	0	theme	DNA	910:912	arg1	%					955:955	48.7 mol%	947:955	48.7 mol%	947:955	The DNA G+C content was determined to be 48.7 mol%.					
30715624	8	0	theme	DNA	910:912	arg1	content					918:924	The DNA G+C content	906:924	The DNA G+C content	906:924	The DNA G+C content was determined to be 48.7 mol%.					
30715624	8	1	theme	G+C	914:916	arg1	%					955:955	48.7 mol%	947:955	48.7 mol%	947:955	The DNA G+C content was determined to be 48.7 mol%.					
30715624	8	1	theme	G+C	914:916	arg1	content					918:924	The DNA G+C content	906:924	The DNA G+C content	906:924	The DNA G+C content was determined to be 48.7 mol%.					
30715624	5	2	from	Pontibacter	550:560	arg1	Cytophagaceae					576:588	the family Cytophagaceae	565:588	the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter	565:691	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	3	3	dep	%	366:366	arg1	5.5					363:365	5.5	363:365	5.5	363:365	The strain can grow at 7-37 °C, pH 6.0-8.0, and can tolerate up to 5.5% (w/v) NaCl concentration.					
30715624	3	3	dep	%	366:366	arg1	w/v					369:371	w/v	369:371	w/v	369:371	The strain can grow at 7-37 °C, pH 6.0-8.0, and can tolerate up to 5.5% (w/v) NaCl concentration.					
30715624	6	4	theme	iso-C17:0	776:784	arg1	3-OH					786:789	iso-C17:0 3-OH	776:789	iso-C17:0 3-OH	776:789	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	4	theme	iso-C17:0	776:784	arg1	acids					725:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	5	theme	iso-C17:1	824:832	arg1	B					850:850	iso-C17:1 I/anteiso-C17:1 B	824:850	iso-C17:1 I/anteiso-C17:1 B	824:850	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	5	6	theme	genus	675:679	arg1	Pontibacter					681:691	the genus Pontibacter	671:691	the genus Pontibacter	671:691	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	2	7	theme	negative	107:114	arg1	strain					186:191	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain	94:191	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain	94:191	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	2	8	theme	University	278:287	arg1	field					289:293	a Seoul Women's University field	262:293	a Seoul Women's University field	262:293	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	2	9	attach	isolated	219:226	arg1	sample					240:245	a soil sample	233:245	a soil sample collected from a Seoul Women's University field	233:293	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	2	9	attach	isolated	219:226	arg2	strain					186:191	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain	94:191	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain	94:191	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	5	10	theme	Pontibacter	681:691	arg1	strains					660:666	other type strains	649:666	other type strains of the genus Pontibacter	649:691	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	10	11	theme	phylogenetic	1062:1073	arg1	analyses					1075:1082	phenotypic, chemotaxonomic and phylogenetic analyses	1031:1082	phenotypic, chemotaxonomic and phylogenetic analyses	1031:1082	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	6	12	theme	fatty	719:723	arg1	anteiso-C15:0					761:773	anteiso-C15:0	761:773	anteiso-C15:0	761:773	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	12	theme	fatty	719:723	arg1	3-OH					786:789	iso-C17:0 3-OH	776:789	iso-C17:0 3-OH	776:789	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	12	theme	fatty	719:723	arg1	feature					802:808	summed feature 4	795:810	summed feature 4	795:810	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	12	theme	fatty	719:723	arg1	iso-C15:0					750:758	iso-C15:0	750:758	iso-C15:0	750:758	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	12	theme	fatty	719:723	arg1	acids					725:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	10	13	theme	Pontibacter	1216:1226	arg1	sp					1243:1244	the name Pontibacter chitinilyticus sp	1207:1244	the name Pontibacter chitinilyticus sp	1207:1244	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	5	14	dep	92.9	638:641	arg1	to					635:636	to	635:636	to	635:636	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	5	15	theme	family	569:574	arg1	Cytophagaceae					576:588	the family Cytophagaceae	565:588	the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter	565:691	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	10	16	from	Bacteroidetes	1182:1194	arg1	species					1135:1141	a novel species	1127:1141	a novel species	1127:1141	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	3	17	theme	NaCl	374:377	arg1	concentration					379:391	up to 5.5% (w/v) NaCl concentration	357:391	up to 5.5% (w/v) NaCl concentration	357:391	The strain can grow at 7-37 °C, pH 6.0-8.0, and can tolerate up to 5.5% (w/v) NaCl concentration.					
30715624	10	18	theme	chemotaxonomic	1043:1056	arg1	analyses					1075:1082	phenotypic, chemotaxonomic and phylogenetic analyses	1031:1082	phenotypic, chemotaxonomic and phylogenetic analyses	1031:1082	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	3	19	dep	5.5	363:365	arg1	to					360:361	to	360:361	to	360:361	The strain can grow at 7-37 °C, pH 6.0-8.0, and can tolerate up to 5.5% (w/v) NaCl concentration.					
30715624	2	20	theme	Seoul	264:268	arg1	Women					270:274	a Seoul Women's	262:276	a Seoul Women's University field	262:293	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	5	21	theme	16S	463:465	arg1	sequences					477:485	16S rRNA gene sequences	463:485	16S rRNA gene sequences	463:485	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	0	22	theme	chitinilyticus	12:25	arg1	sp					27:28	Pontibacter chitinilyticus sp	0:28	Pontibacter chitinilyticus sp.	0:29	Pontibacter chitinilyticus sp.					
30715624	6	23	theme	I/anteiso-C17:1	834:848	arg1	B					850:850	iso-C17:1 I/anteiso-C17:1 B	824:850	iso-C17:1 I/anteiso-C17:1 B	824:850	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	5	24	theme	sequence	595:602	arg1	similarities					604:615	sequence similarities	595:615	sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter	595:691	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	8	25	theme	48.7 mol	947:954	arg1	%					955:955	48.7 mol%	947:955	48.7 mol%	947:955	The DNA G+C content was determined to be 48.7 mol%.					
30715624	8	25	theme	48.7 mol	947:954	arg1	content					918:924	The DNA G+C content	906:924	The DNA G+C content	906:924	The DNA G+C content was determined to be 48.7 mol%.					
30715624	5	26	theme	rRNA	467:470	arg1	sequences					477:485	16S rRNA gene sequences	463:485	16S rRNA gene sequences	463:485	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	0	27	theme	Pontibacter	0:10	arg1	sp					27:28	Pontibacter chitinilyticus sp	0:28	Pontibacter chitinilyticus sp.	0:29	Pontibacter chitinilyticus sp.					
30715624	10	28	theme	strain	1085:1090	arg1	17gy-14T					1092:1099	strain 17gy-14T	1085:1099	strain 17gy-14T	1085:1099	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	5	29	theme	type	655:658	arg1	strains					660:666	other type strains	649:666	other type strains of the genus Pontibacter	649:691	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	5	30	theme	genus	544:548	arg1	Pontibacter					550:560	the genus Pontibacter	540:560	the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter	540:691	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	6	31	theme	cellular	710:717	arg1	anteiso-C15:0					761:773	anteiso-C15:0	761:773	anteiso-C15:0	761:773	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	31	theme	cellular	710:717	arg1	3-OH					786:789	iso-C17:0 3-OH	776:789	iso-C17:0 3-OH	776:789	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	31	theme	cellular	710:717	arg1	feature					802:808	summed feature 4	795:810	summed feature 4	795:810	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	31	theme	cellular	710:717	arg1	iso-C15:0					750:758	iso-C15:0	750:758	iso-C15:0	750:758	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	31	theme	cellular	710:717	arg1	acids					725:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	4	32	theme	Flexirubin-type	394:408	arg1	pigments					410:417	Flexirubin-type pigments	394:417	Flexirubin-type pigments	394:417	Flexirubin-type pigments were absent.					
30715624	5	33	theme	Phylogenetic	432:443	arg1	analysis					445:452	Phylogenetic analysis	432:452	Phylogenetic analysis based on 16S rRNA gene sequences	432:485	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	2	34	theme	soil	235:238	arg1	sample					240:245	a soil sample	233:245	a soil sample collected from a Seoul Women's University field	233:293	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	10	35	theme	Pontibacter	1156:1166	arg1	species					1135:1141	a novel species	1127:1141	a novel species	1127:1141	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	2	36	theme	bacterial	176:184	arg1	strain					186:191	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain	94:191	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain	94:191	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	12	37	theme	=KCTC	1294:1298	arg1	113056T					1312:1318	=KCTC 52914T=NRBC 113056T	1294:1318	=KCTC 52914T=NRBC 113056T	1294:1318	The type strain is 17gy-14T (=KCTC 52914T=NRBC 113056T).					
30715624	12	37	theme	=KCTC	1294:1298	arg1	17gy-14T					1284:1291	17gy-14T	1284:1291	17gy-14T (=KCTC 52914T=NRBC 113056T)	1284:1319	The type strain is 17gy-14T (=KCTC 52914T=NRBC 113056T).					
30715624	2	38	dep	negative	107:114	arg1	non-motile					117:126	non-motile	117:126	non-motile	117:126	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	2	38	dep	negative	107:114	arg1	rod-shaped					165:174	rod-shaped	165:174	rod-shaped	165:174	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	2	38	dep	negative	107:114	arg1	asporogenous					151:162	asporogenous	151:162	asporogenous	151:162	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	2	38	dep	negative	107:114	arg1	orange-red-pigmented					129:148	orange-red-pigmented	129:148	orange-red-pigmented	129:148	A Gram-stain negative, non-motile, orange-red-pigmented, asporogenous, rod-shaped bacterial strain, designated 17gy-14T, was isolated from a soil sample collected from a Seoul Women's University field.					
30715624	12	39	theme	52914T=NRBC	1300:1310	arg1	113056T					1312:1318	=KCTC 52914T=NRBC 113056T	1294:1318	=KCTC 52914T=NRBC 113056T	1294:1318	The type strain is 17gy-14T (=KCTC 52914T=NRBC 113056T).					
30715624	12	39	theme	52914T=NRBC	1300:1310	arg1	17gy-14T					1284:1291	17gy-14T	1284:1291	17gy-14T (=KCTC 52914T=NRBC 113056T)	1284:1319	The type strain is 17gy-14T (=KCTC 52914T=NRBC 113056T).					
30715624	5	40	theme	strain	506:511	arg1	strain					522:527	the strain 17gy-14T strain	502:527	the strain 17gy-14T strain	502:527	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	10	41	from	species	1135:1141	arg1	Bacteroidetes					1182:1194	the phylum Bacteroidetes	1171:1194	the phylum Bacteroidetes	1171:1194	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	3	42	dep	concentration	379:391	arg1	%					366:366	%	366:366	%	366:366	The strain can grow at 7-37 °C, pH 6.0-8.0, and can tolerate up to 5.5% (w/v) NaCl concentration.					
30715624	5	43	theme	17gy-14T	513:520	arg1	strain					522:527	the strain 17gy-14T strain	502:527	the strain 17gy-14T strain	502:527	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	9	44	theme	major	962:966	arg1	lipid					974:978	The major polar lipid	958:978	The major polar lipid	958:978	The major polar lipid was found to be phosphatidylethanolamine.					
30715624	9	44	theme	major	962:966	arg1	phosphatidylethanolamine					996:1019	phosphatidylethanolamine	996:1019	phosphatidylethanolamine	996:1019	The major polar lipid was found to be phosphatidylethanolamine.					
30715624	10	45	from	Pontibacter	1156:1166	arg1	Bacteroidetes					1182:1194	the phylum Bacteroidetes	1171:1194	the phylum Bacteroidetes	1171:1194	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	6	46	theme	summed	795:800	arg1	feature					802:808	summed feature 4	795:810	summed feature 4	795:810	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	46	theme	summed	795:800	arg1	acids					725:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	12	47	theme	type	1269:1272	arg1	strain					1274:1279	The type strain	1265:1279	The type strain	1265:1279	The type strain is 17gy-14T (=KCTC 52914T=NRBC 113056T).					
30715624	12	47	theme	type	1269:1272	arg1	17gy-14T					1284:1291	17gy-14T	1284:1291	17gy-14T (=KCTC 52914T=NRBC 113056T)	1284:1319	The type strain is 17gy-14T (=KCTC 52914T=NRBC 113056T).					
30715624	6	48	theme	predominant	698:708	arg1	anteiso-C15:0					761:773	anteiso-C15:0	761:773	anteiso-C15:0	761:773	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	48	theme	predominant	698:708	arg1	3-OH					786:789	iso-C17:0 3-OH	776:789	iso-C17:0 3-OH	776:789	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	48	theme	predominant	698:708	arg1	feature					802:808	summed feature 4	795:810	summed feature 4	795:810	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	48	theme	predominant	698:708	arg1	iso-C15:0					750:758	iso-C15:0	750:758	iso-C15:0	750:758	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	6	48	theme	predominant	698:708	arg1	acids					725:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids	694:729	The predominant cellular fatty acids were identified as iso-C15:0, anteiso-C15:0, iso-C17:0 3-OH and summed feature 4 (comprising iso-C17:1 I/anteiso-C17:1 B).					
30715624	5	49	with	Cytophagaceae	576:588	arg1	similarities					604:615	sequence similarities	595:615	sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter	595:691	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	9	50	theme	polar	968:972	arg1	lipid					974:978	The major polar lipid	958:978	The major polar lipid	958:978	The major polar lipid was found to be phosphatidylethanolamine.					
30715624	9	50	theme	polar	968:972	arg1	phosphatidylethanolamine					996:1019	phosphatidylethanolamine	996:1019	phosphatidylethanolamine	996:1019	The major polar lipid was found to be phosphatidylethanolamine.					
30715624	5	51	theme	other	649:653	arg1	strains					660:666	other type strains	649:666	other type strains of the genus Pontibacter	649:691	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	1	52	theme	chitin-hydrolysing	45:62	arg1	bacterium					64:72	a novel chitin-hydrolysing bacterium	37:72	a novel chitin-hydrolysing bacterium	37:72	nov., a novel chitin-hydrolysing bacterium isolated from soil.					
30715624	1	52	theme	chitin-hydrolysing	45:62	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., a novel chitin-hydrolysing bacterium isolated from soil.					
30715624	5	53	theme	gene	472:475	arg1	sequences					477:485	16S rRNA gene sequences	463:485	16S rRNA gene sequences	463:485	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain 17gy-14T strain belongs to the genus Pontibacter in the family Cytophagaceae with sequence similarities ranging from 95.4 to 92.9% with other type strains of the genus Pontibacter.					
30715624	10	54	theme	phylum	1175:1180	arg1	Bacteroidetes					1182:1194	the phylum Bacteroidetes	1171:1194	the phylum Bacteroidetes	1171:1194	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	10	55	theme	chitinilyticus	1228:1241	arg1	sp					1243:1244	the name Pontibacter chitinilyticus sp	1207:1244	the name Pontibacter chitinilyticus sp	1207:1244	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	7	56	theme	predominant	858:868	arg1	MK-7					900:903	MK-7	900:903	MK-7	900:903	The predominant menaquinone was identified as MK-7.					
30715624	7	56	theme	predominant	858:868	arg1	menaquinone					870:880	The predominant menaquinone	854:880	The predominant menaquinone	854:880	The predominant menaquinone was identified as MK-7.					
30715624	10	57	theme	novel	1129:1133	arg1	species					1135:1141	a novel species	1127:1141	a novel species	1127:1141	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	10	58	theme	genus	1150:1154	arg1	Pontibacter					1156:1166	the genus Pontibacter	1146:1166	the genus Pontibacter in the phylum Bacteroidetes	1146:1194	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	10	59	theme	phenotypic	1031:1040	arg1	analyses					1075:1082	phenotypic, chemotaxonomic and phylogenetic analyses	1031:1082	phenotypic, chemotaxonomic and phylogenetic analyses	1031:1082	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30715624	1	60	theme	novel	39:43	arg1	bacterium					64:72	a novel chitin-hydrolysing bacterium	37:72	a novel chitin-hydrolysing bacterium	37:72	nov., a novel chitin-hydrolysing bacterium isolated from soil.					
30715624	1	60	theme	novel	39:43	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., a novel chitin-hydrolysing bacterium isolated from soil.					
30715624	10	61	theme	name	1211:1214	arg1	sp					1243:1244	the name Pontibacter chitinilyticus sp	1207:1244	the name Pontibacter chitinilyticus sp	1207:1244	Based on phenotypic, chemotaxonomic and phylogenetic analyses, strain 17gy-14T is concluded to represent a novel species of the genus Pontibacter in the phylum Bacteroidetes, for which the name Pontibacter chitinilyticus sp.					
30445022	10	0	theme	fatty	1598:1602	arg1	acids					1604:1608	shorter chain fatty acids	1584:1608	shorter chain fatty acids (from C6:0 to C10:0)	1584:1629	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	10	1	from	proportions	1508:1518	arg1	PL					1542:1543	PL	1542:1543	PL	1542:1543	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	4	2	theme	crapemyrtle	599:609	arg1	scale					616:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	4	2	theme	crapemyrtle	599:609	arg1	lagerstroemiae					637:650	lagerstroemiae	637:650	lagerstroemiae	637:650	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	4	2	theme	crapemyrtle	599:609	arg1	pest					716:719	a newly introduced scale pest	691:719	a newly introduced scale pest of crapemyrtles, Lagerstroemia spp	691:754	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	6	3	theme	previous	785:792	arg1	study					794:798	Our previous study	781:798	Our previous study concerning the cold tolerance of this pest	781:841	Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points.					
30445022	3	4	theme	scale	551:555	arg1	insects					557:563	scale insects	551:563	scale insects in the superfamily Coccoidea	551:592	Although the mechanisms of seasonal adaptation to low temperatures have been studied for insects in different taxa, none of these mechanisms have been investigated in scale insects in the superfamily Coccoidea.					
30445022	1	5	theme	important	159:167	arg1	factors					177:183	the most important abiotic factors	150:183	the most important abiotic factors influencing the adaptation and diversification of insects	150:241	Temperature is one of the most important abiotic factors influencing the adaptation and diversification of insects.					
30445022	8	6	theme	model	1137:1141	arg1	system					1143:1148	a model system	1135:1148	a model system	1135:1148	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	8	6	theme	model	1137:1141	arg1	lagerstroemiae					1117:1130	A. lagerstroemiae	1114:1130	A. lagerstroemiae	1114:1130	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	10	7	from	acids	1533:1537	arg1	PL					1542:1543	PL	1542:1543	PL	1542:1543	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	8	8	used	used	1109:1112	arg2	we					1106:1107	we	1106:1107	we	1106:1107	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	3	9	theme	superfamily	572:582	arg1	Coccoidea					584:592	the superfamily Coccoidea	568:592	the superfamily Coccoidea	568:592	Although the mechanisms of seasonal adaptation to low temperatures have been studied for insects in different taxa, none of these mechanisms have been investigated in scale insects in the superfamily Coccoidea.					
30445022	1	10	theme	factors	177:183	arg1	one					143:145	one	143:145	one	143:145	Temperature is one of the most important abiotic factors influencing the adaptation and diversification of insects.					
30445022	1	10	theme	factors	177:183	arg1	factors					177:183	the most important abiotic factors	150:183	the most important abiotic factors influencing the adaptation and diversification of insects	150:241	Temperature is one of the most important abiotic factors influencing the adaptation and diversification of insects.					
30445022	0	11	theme	cold	112:115	arg1	tolerance					117:125	seasonally altered cold tolerance	93:125	seasonally altered cold tolerance	93:125	Physiology of crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana), associated with seasonally altered cold tolerance.					
30445022	8	12	theme	scale	1239:1243	arg1	insects					1245:1251	scale insects	1239:1251	scale insects	1239:1251	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	10	13	theme	acids	1533:1537	arg1	proportions					1508:1518	The proportions	1504:1518	The proportions of the fatty acids in PL	1504:1543	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	10	13	theme	acids	1533:1537	arg1	similar					1550:1556	similar	1550:1556	similar	1550:1556	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	7	14	theme	same	1028:1031	arg1	levels					1033:1038	the same levels	1024:1038	the same levels of mortality at lower temperatures	1024:1073	In addition, time required to achieve the same levels of mortality at lower temperatures also increased.					
30445022	10	15	theme	fatty	1667:1671	arg1	acids					1673:1677	longer chain fatty acids	1654:1677	longer chain fatty acids	1654:1677	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	4	16	theme	introduced	699:708	arg1	scale					616:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	4	16	theme	introduced	699:708	arg1	pest					716:719	a newly introduced scale pest	691:719	a newly introduced scale pest of crapemyrtles, Lagerstroemia spp	691:754	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	10	17	theme	longer	1654:1659	arg1	acids					1673:1677	longer chain fatty acids	1654:1677	longer chain fatty acids	1654:1677	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	9	18	theme	early	1442:1446	arg1	spring					1448:1453	early spring	1442:1453	early spring	1442:1453	Results suggested that water content was lower in winter and early spring than in summer and early fall (40.8% vs. 63.3%).					
30445022	11	19	theme	those	1896:1900	arg1	19-times					1884:1891	19-times	1884:1891	19-times of those in March 2016	1884:1914	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	19	theme	those	1896:1900	arg1	4.5-times					1920:1928	4.5-times	1920:1928	4.5-times of those in September 2016	1920:1955	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	12	20	theme	scale	2118:2122	arg1	insects					2124:2130	other scale insects	2112:2130	other scale insects	2112:2130	Results from this study provide a better understanding on how A. lagerstroemiae overwinters, which may give insights into the overwintering strategies of other scale insects.					
30445022	2	21	theme	complex	256:262	arg1	mechanisms					278:287	Diverse and complex physiological mechanisms	244:287	Diverse and complex physiological mechanisms	244:287	Diverse and complex physiological mechanisms have evolved to help insects adapt to seasonal changes in temperature and prevent cold injury.					
30445022	11	22	from	March	1905:1909	arg1	19-times					1884:1891	19-times	1884:1891	19-times of those in March 2016	1884:1914	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	22	from	March	1905:1909	arg1	4.5-times					1920:1928	4.5-times	1920:1928	4.5-times of those in September 2016	1920:1955	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	2	23	theme	Diverse	244:250	arg1	mechanisms					278:287	Diverse and complex physiological mechanisms	244:287	Diverse and complex physiological mechanisms	244:287	Diverse and complex physiological mechanisms have evolved to help insects adapt to seasonal changes in temperature and prevent cold injury.					
30445022	8	24	from	tolerance	1226:1234	arg1	insects					1245:1251	scale insects	1239:1251	scale insects	1239:1251	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	12	25	theme	overwintering	2084:2096	arg1	strategies					2098:2107	the overwintering strategies	2080:2107	the overwintering strategies of other scale insects	2080:2130	Results from this study provide a better understanding on how A. lagerstroemiae overwinters, which may give insights into the overwintering strategies of other scale insects.					
30445022	8	26	theme	lipid	1282:1286	arg1	content					1288:1294	lipid content	1282:1294	lipid content	1282:1294	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	6	27	theme	supercooling	965:976	arg1	points					978:983	supercooling points	965:983	supercooling points	965:983	Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points.					
30445022	11	28	from	19-times	1884:1891	arg1	September					1942:1950	September	1942:1950	September	1942:1950	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	28	from	19-times	1884:1891	arg1	March					1905:1909	March	1905:1909	March	1905:1909	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	8	29	theme	acid	1306:1309	arg1	composition					1311:1321	fatty acid composition	1300:1321	fatty acid composition	1300:1321	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	3	30	theme	low	434:436	arg1	temperatures					438:449	low temperatures	434:449	low temperatures	434:449	Although the mechanisms of seasonal adaptation to low temperatures have been studied for insects in different taxa, none of these mechanisms have been investigated in scale insects in the superfamily Coccoidea.					
30445022	8	31	theme	cryoprotective	1328:1341	arg1	polyols					1343:1349	cryoprotective polyols	1328:1349	cryoprotective polyols	1328:1349	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	0	32	theme	crapemyrtle	14:24	arg1	scale					31:35	crapemyrtle bark scale	14:35	crapemyrtle bark scale	14:35	Physiology of crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana), associated with seasonally altered cold tolerance.					
30445022	0	32	theme	crapemyrtle	14:24	arg1	lagerstroemiae					52:65	Acanthococcus lagerstroemiae	38:65	Acanthococcus lagerstroemiae (Kuwana)	38:74	Physiology of crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana), associated with seasonally altered cold tolerance.					
30445022	3	33	theme	adaptation	420:429	arg1	mechanisms					397:406	the mechanisms	393:406	the mechanisms of seasonal adaptation to low temperatures	393:449	Although the mechanisms of seasonal adaptation to low temperatures have been studied for insects in different taxa, none of these mechanisms have been investigated in scale insects in the superfamily Coccoidea.					
30445022	7	34	from	temperatures	1062:1073	arg1	levels					1033:1038	the same levels	1024:1038	the same levels of mortality at lower temperatures	1024:1073	In addition, time required to achieve the same levels of mortality at lower temperatures also increased.					
30445022	0	35	theme	scale	31:35	arg1	Physiology					0:9	Physiology	0:9	Physiology of crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana),	0:75	Physiology of crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana), associated with seasonally altered cold tolerance.					
30445022	6	36	theme	pest	838:841	arg1	tolerance					820:828	the cold tolerance	811:828	the cold tolerance of this pest	811:841	Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points.					
30445022	10	37	theme	chain	1592:1596	arg1	acids					1604:1608	shorter chain fatty acids	1584:1608	shorter chain fatty acids (from C6:0 to C10:0)	1584:1629	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	4	38	dep	lagerstroemiae	637:650	arg1	Hemiptera					662:670	Hemiptera	662:670	Hemiptera	662:670	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	1	39	dep	adaptation	201:210	arg1	the					197:199	the	197:199	the	197:199	Temperature is one of the most important abiotic factors influencing the adaptation and diversification of insects.					
30445022	3	40	theme	different	484:492	arg1	taxa					494:497	different taxa	484:497	different taxa	484:497	Although the mechanisms of seasonal adaptation to low temperatures have been studied for insects in different taxa, none of these mechanisms have been investigated in scale insects in the superfamily Coccoidea.					
30445022	6	41	theme	cold	815:818	arg1	tolerance					820:828	the cold tolerance	811:828	the cold tolerance of this pest	811:841	Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points.					
30445022	4	42	theme	bark	611:614	arg1	scale					616:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	4	42	theme	bark	611:614	arg1	lagerstroemiae					637:650	lagerstroemiae	637:650	lagerstroemiae	637:650	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	4	42	theme	bark	611:614	arg1	pest					716:719	a newly introduced scale pest	691:719	a newly introduced scale pest of crapemyrtles, Lagerstroemia spp	691:754	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	11	43	theme	measured	1722:1729	arg1	myo-inositol					1783:1794	myo-inositol	1783:1794	myo-inositol	1783:1794	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	43	theme	measured	1722:1729	arg1	d-trehalose					1801:1811	d-trehalose	1801:1811	d-trehalose	1801:1811	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	43	theme	measured	1722:1729	arg1	d-mannitol					1771:1780	d-mannitol	1771:1780	d-mannitol	1771:1780	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	43	theme	measured	1722:1729	arg1	glycerol					1761:1768	glycerol	1761:1768	glycerol	1761:1768	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	43	theme	measured	1722:1729	arg1	polyols					1731:1737	polyols	1731:1737	polyols	1731:1737	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	1	44	theme	insects	235:241	arg1	diversification					216:230	diversification	216:230	diversification	216:230	Temperature is one of the most important abiotic factors influencing the adaptation and diversification of insects.					
30445022	1	44	theme	insects	235:241	arg1	adaptation					201:210	adaptation	201:210	adaptation	201:210	Temperature is one of the most important abiotic factors influencing the adaptation and diversification of insects.					
30445022	11	45	theme	those	1933:1937	arg1	19-times					1884:1891	19-times	1884:1891	19-times of those in March 2016	1884:1914	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	45	theme	those	1933:1937	arg1	4.5-times					1920:1928	4.5-times	1920:1928	4.5-times of those in September 2016	1920:1955	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	2	46	from	changes	336:342	arg1	temperature					347:357	temperature	347:357	temperature	347:357	Diverse and complex physiological mechanisms have evolved to help insects adapt to seasonal changes in temperature and prevent cold injury.					
30445022	6	47	dep	winter	874:879	arg1	summer					864:869	summer	864:869	summer	864:869	Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points.					
30445022	6	48	dep	A.	882:883	arg1	lagerstroemiae					885:898	A. lagerstroemiae	882:898	A. lagerstroemiae	882:898	Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points.					
30445022	7	49	theme	lower	1056:1060	arg1	temperatures					1062:1073	lower temperatures	1056:1073	lower temperatures	1056:1073	In addition, time required to achieve the same levels of mortality at lower temperatures also increased.					
30445022	11	50	theme	d-mannitol	1828:1837	arg1	highest					1848:1854	highest	1848:1854	highest	1848:1854	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	50	theme	d-mannitol	1828:1837	arg1	levels					1818:1823	the levels	1814:1823	the levels of d-mannitol	1814:1837	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	9	51	theme	water	1404:1408	arg1	content					1410:1416	water content	1404:1416	water content	1404:1416	Results suggested that water content was lower in winter and early spring than in summer and early fall (40.8% vs. 63.3%).					
30445022	2	52	dep	Diverse	244:250	arg1	physiological					264:276	physiological	264:276	physiological	264:276	Diverse and complex physiological mechanisms have evolved to help insects adapt to seasonal changes in temperature and prevent cold injury.					
30445022	7	53	theme	mortality	1043:1051	arg1	levels					1033:1038	the same levels	1024:1038	the same levels of mortality at lower temperatures	1024:1073	In addition, time required to achieve the same levels of mortality at lower temperatures also increased.					
30445022	1	54	theme	abiotic	169:175	arg1	factors					177:183	the most important abiotic factors	150:183	the most important abiotic factors influencing the adaptation and diversification of insects	150:241	Temperature is one of the most important abiotic factors influencing the adaptation and diversification of insects.					
30445022	6	55	theme	5 °C	947:950	arg1	reduction					952:960	a 5 °C reduction	945:960	a 5 °C reduction of supercooling points	945:983	Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points.					
30445022	10	56	dep	C14:0	1685:1689	arg1	C18:0					1694:1698	C18:0	1694:1698	C18:0	1694:1698	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	10	56	dep	C14:0	1685:1689	arg1	to					1691:1692	to	1691:1692	to	1691:1692	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	11	57	from	September	1942:1950	arg1	19-times					1884:1891	19-times	1884:1891	19-times of those in March 2016	1884:1914	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	57	from	September	1942:1950	arg1	4.5-times					1920:1928	4.5-times	1920:1928	4.5-times of those in September 2016	1920:1955	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	5	58	dep	Myrtales	758:765	arg1	Lythraceae					768:777	Lythraceae	768:777	Myrtales: Lythraceae	758:777	(Myrtales: Lythraceae).					
30445022	8	59	theme	cold	1221:1224	arg1	tolerance					1226:1234	cold tolerance	1221:1234	cold tolerance in scale insects	1221:1251	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	6	60	theme	lower	922:926	arg1	temperature					928:938	lower temperature	922:938	lower temperature	922:938	Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points.					
30445022	10	61	theme	fatty	1527:1531	arg1	acids					1533:1537	the fatty acids	1523:1537	the fatty acids in PL	1523:1543	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	11	62	from	4.5-times	1920:1928	arg1	September					1942:1950	September	1942:1950	September	1942:1950	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	11	62	from	4.5-times	1920:1928	arg1	March					1905:1909	March	1905:1909	March	1905:1909	Among all measured polyols and sugars, including glycerol, d-mannitol, myo-inositol, and d-trehalose, the levels of d-mannitol were the highest in January 2016, which were 19-times of those in March 2016 and 4.5-times of those in September 2016.					
30445022	10	63	theme	chain	1661:1665	arg1	acids					1673:1677	longer chain fatty acids	1654:1677	longer chain fatty acids	1654:1677	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	8	64	from	changes	1210:1216	arg1	tolerance					1226:1234	cold tolerance	1221:1234	cold tolerance in scale insects	1221:1251	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	8	65	theme	physiological	1169:1181	arg1	mechanisms					1183:1192	the physiological mechanisms	1165:1192	the physiological mechanisms correlated with changes in cold tolerance in scale insects	1165:1251	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	12	66	theme	insects	2124:2130	arg1	strategies					2098:2107	the overwintering strategies	2080:2107	the overwintering strategies of other scale insects	2080:2130	Results from this study provide a better understanding on how A. lagerstroemiae overwinters, which may give insights into the overwintering strategies of other scale insects.					
30445022	10	67	dep	increased	1631:1639	arg1	decreased					1701:1709	decreased	1701:1709	increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased	1631:1709	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	10	67	dep	increased	1631:1639	arg1	C14:0					1685:1689	C14:0	1685:1689	C14:0	1685:1689	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	3	68	from	insects	557:563	arg1	Coccoidea					584:592	the superfamily Coccoidea	568:592	the superfamily Coccoidea	568:592	Although the mechanisms of seasonal adaptation to low temperatures have been studied for insects in different taxa, none of these mechanisms have been investigated in scale insects in the superfamily Coccoidea.					
30445022	4	69	theme	scale	710:714	arg1	scale					616:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	4	69	theme	scale	710:714	arg1	pest					716:719	a newly introduced scale pest	691:719	a newly introduced scale pest of crapemyrtles, Lagerstroemia spp	691:754	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	8	70	theme	water	1267:1271	arg1	content					1273:1279	water content	1267:1279	water content	1267:1279	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	6	71	theme	points	978:983	arg1	reduction					952:960	a 5 °C reduction	945:960	a 5 °C reduction of supercooling points	945:983	Our previous study concerning the cold tolerance of this pest suggested that, from summer to winter, A. lagerstroemiae seasonally adapted to lower temperature with a 5 °C reduction of supercooling points.					
30445022	12	72	theme	other	2112:2116	arg1	insects					2124:2130	other scale insects	2112:2130	other scale insects	2112:2130	Results from this study provide a better understanding on how A. lagerstroemiae overwinters, which may give insights into the overwintering strategies of other scale insects.					
30445022	10	73	dep	C10:0	1624:1628	arg1	C6:0					1616:1619	C6:0	1616:1619	C6:0	1616:1619	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	4	74	theme	spp	752:754	arg1	scale					616:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale	595:620	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	4	74	theme	spp	752:754	arg1	pest					716:719	a newly introduced scale pest	691:719	a newly introduced scale pest of crapemyrtles, Lagerstroemia spp	691:754	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	0	75	theme	altered	104:110	arg1	tolerance					117:125	seasonally altered cold tolerance	93:125	seasonally altered cold tolerance	93:125	Physiology of crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana), associated with seasonally altered cold tolerance.					
30445022	8	76	theme	fatty	1300:1304	arg1	composition					1311:1321	fatty acid composition	1300:1321	fatty acid composition	1300:1321	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	9	77	dep	early	1474:1478	arg1	fall					1480:1483	fall	1480:1483	fall	1480:1483	Results suggested that water content was lower in winter and early spring than in summer and early fall (40.8% vs. 63.3%).					
30445022	9	77	dep	early	1474:1478	arg1	%					1490:1490	40.8%	1486:1490	40.8%	1486:1490	Results suggested that water content was lower in winter and early spring than in summer and early fall (40.8% vs. 63.3%).					
30445022	9	77	dep	early	1474:1478	arg1	%					1500:1500	63.3%	1496:1500	63.3%	1496:1500	Results suggested that water content was lower in winter and early spring than in summer and early fall (40.8% vs. 63.3%).					
30445022	0	78	theme	bark	26:29	arg1	scale					31:35	crapemyrtle bark scale	14:35	crapemyrtle bark scale	14:35	Physiology of crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana), associated with seasonally altered cold tolerance.					
30445022	0	78	theme	bark	26:29	arg1	lagerstroemiae					52:65	Acanthococcus lagerstroemiae	38:65	Acanthococcus lagerstroemiae (Kuwana)	38:74	Physiology of crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana), associated with seasonally altered cold tolerance.					
30445022	8	79	dep	content	1273:1279	arg1	month					1374:1378	every other month	1362:1378	every other month	1362:1378	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	10	80	from	PL	1542:1543	arg1	proportions					1508:1518	The proportions	1504:1518	The proportions of the fatty acids in PL	1504:1543	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	10	80	from	PL	1542:1543	arg1	similar					1550:1556	similar	1550:1556	similar	1550:1556	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	3	81	theme	seasonal	411:418	arg1	adaptation					420:429	seasonal adaptation	411:429	seasonal adaptation to low temperatures	411:449	Although the mechanisms of seasonal adaptation to low temperatures have been studied for insects in different taxa, none of these mechanisms have been investigated in scale insects in the superfamily Coccoidea.					
30445022	12	82	theme	better	1992:1997	arg1	understanding					1999:2011	a better understanding	1990:2011	a better understanding	1990:2011	Results from this study provide a better understanding on how A. lagerstroemiae overwinters, which may give insights into the overwintering strategies of other scale insects.					
30445022	10	83	theme	shorter	1584:1590	arg1	acids					1604:1608	shorter chain fatty acids	1584:1608	shorter chain fatty acids (from C6:0 to C10:0)	1584:1629	The proportions of the fatty acids in PL were similar over seasons, but in TAG, shorter chain fatty acids (from C6:0 to C10:0) increased in winter as longer chain fatty acids (from C14:0 to C18:0) decreased.					
30445022	8	84	theme	other	1368:1372	arg1	month					1374:1378	every other month	1362:1378	every other month	1362:1378	In this study, we used A. lagerstroemiae as a model system to investigate the physiological mechanisms correlated with changes in cold tolerance in scale insects, by measuring water content, lipid content and fatty acid composition, and cryoprotective polyols and sugars every other month.					
30445022	2	85	theme	seasonal	327:334	arg1	changes					336:342	seasonal changes	327:342	seasonal changes in temperature	327:357	Diverse and complex physiological mechanisms have evolved to help insects adapt to seasonal changes in temperature and prevent cold injury.					
30445022	4	86	dep	Hemiptera	662:670	arg1	Eriococcidae					673:684	Eriococcidae	673:684	Eriococcidae	673:684	The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is a newly introduced scale pest of crapemyrtles, Lagerstroemia spp.					
30445022	2	87	theme	cold	371:374	arg1	injury					376:381	cold injury	371:381	cold injury	371:381	Diverse and complex physiological mechanisms have evolved to help insects adapt to seasonal changes in temperature and prevent cold injury.					
30445022	12	88	from	study	1976:1980	arg1	Results					1958:1964	Results	1958:1964	Results from this study	1958:1980	Results from this study provide a better understanding on how A. lagerstroemiae overwinters, which may give insights into the overwintering strategies of other scale insects.					
29843473	4	0	theme	cycle	524:528	arg1	steps					487:491	the early steps	477:491	the early steps of the bacteriophage infection cycle	477:528	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	7	1	theme	permissive	1103:1112	arg1	temperatures					1114:1125	permissive temperatures	1103:1125	permissive temperatures	1103:1125	Moreover, we found that HK620 irreversibly adsorbed to the LPS receptor via its TSP at restrictive low temperatures, without opening the particle but could eject its DNA at permissive temperatures.					
29843473	5	2	theme	particle	690:697	arg1	opening					699:705	particle opening	690:705	particle opening	690:705	In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620.					
29843473	6	3	theme	comparable	881:890	arg1	velocities					870:879	velocities	870:879	velocities comparable to those found for other podoviruses	870:927	Fluorescence-based monitoring of DNA release showed that HK620 phage particles in vitro ejected their genome at velocities comparable to those found for other podoviruses.					
29843473	6	4	theme	other	911:915	arg1	podoviruses					917:927	other podoviruses	911:927	other podoviruses	911:927	Fluorescence-based monitoring of DNA release showed that HK620 phage particles in vitro ejected their genome at velocities comparable to those found for other podoviruses.					
29843473	6	5	theme	phage	821:825	arg1	particles					827:835	HK620 phage particles	815:835	HK620 phage particles	815:835	Fluorescence-based monitoring of DNA release showed that HK620 phage particles in vitro ejected their genome at velocities comparable to those found for other podoviruses.					
29843473	8	6	theme	DNA	1128:1130	arg1	ejection					1132:1139	DNA ejection	1128:1139	DNA ejection	1128:1139	DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro.					
29843473	8	7	from	HK620	1294:1298	arg1	release					1267:1273	the DNA release	1259:1273	the DNA release from E. coli phage HK620	1259:1298	DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro.					
29843473	5	8	theme	LPS	656:658	arg1	preparations					660:671	LPS preparations	656:671	LPS preparations	656:671	In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620.					
29843473	6	9	theme	HK620	815:819	arg1	particles					827:835	HK620 phage particles	815:835	HK620 phage particles	815:835	Fluorescence-based monitoring of DNA release showed that HK620 phage particles in vitro ejected their genome at velocities comparable to those found for other podoviruses.					
29843473	4	10	theme	phage	603:607	arg1	resistance					609:618	phage resistance	603:618	phage resistance	603:618	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	5	11	theme	E.	733:734	arg1	HK620					751:755	the E. coli podovirus HK620	729:755	the E. coli podovirus HK620	729:755	In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620.					
29843473	2	12	theme	LPS	299:301	arg1	part					291:294	the O-polysaccharide part	270:294	the O-polysaccharide part of LPS	270:301	O-antigen-specific bacteriophages use tailspike proteins (TSP) to recognize and cleave the O-polysaccharide part of LPS.					
29843473	9	13	theme	O-antigen	1424:1432	arg1	structures					1434:1443	O-antigen structures	1424:1443	O-antigen structures	1424:1443	This finding can be significant when optimizing bacteriophage mixtures for therapy, where in natural environments O-antigen structures may rapidly change.					
29843473	9	14	theme	natural	1403:1409	arg1	environments					1411:1422	natural environments	1403:1422	natural environments	1403:1422	This finding can be significant when optimizing bacteriophage mixtures for therapy, where in natural environments O-antigen structures may rapidly change.					
29843473	4	15	theme	infection	514:522	arg1	cycle					524:528	the bacteriophage infection cycle	496:528	the bacteriophage infection cycle	496:528	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	4	16	from	changes	560:566	arg1	range					576:580	host range	571:580	host range	571:580	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	4	16	from	changes	560:566	arg1	occurrence					589:598	the occurrence	585:598	the occurrence of phage resistance	585:618	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	6	17	theme	release	795:801	arg1	monitoring					777:786	Fluorescence-based monitoring	758:786	Fluorescence-based monitoring of DNA release	758:801	Fluorescence-based monitoring of DNA release showed that HK620 phage particles in vitro ejected their genome at velocities comparable to those found for other podoviruses.					
29843473	1	18	theme	Gram-negative	80:92	arg1	bacteria					94:101	Gram-negative bacteria	80:101	Gram-negative bacteria	80:101	Gram-negative bacteria protect themselves with an outermost layer containing lipopolysaccharide (LPS).					
29843473	8	19	dep	stimulated	1152:1161	arg1	dictated					1213:1220	dictated	1213:1220	dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro	1213:1307	DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro.					
29843473	4	20	theme	bacteriophage	500:512	arg1	cycle					524:528	the bacteriophage infection cycle	496:528	the bacteriophage infection cycle	496:528	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	6	21	theme	DNA	791:793	arg1	release					795:801	DNA release	791:801	DNA release	791:801	Fluorescence-based monitoring of DNA release showed that HK620 phage particles in vitro ejected their genome at velocities comparable to those found for other podoviruses.					
29843473	8	22	theme	LPS	1234:1236	arg1	receptor					1238:1245	the LPS receptor	1230:1245	the LPS receptor	1230:1245	DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro.					
29843473	0	23	theme	In	0:1	arg1	Studies					9:15	In Vitro Studies	0:15	In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.	0:78	In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.					
29843473	4	24	theme	host	571:574	arg1	range					576:580	host range	571:580	host range	571:580	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	5	25	from	HK620	751:755	arg1	opening					699:705	particle opening	690:705	particle opening	690:705	In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620.					
29843473	5	25	from	HK620	751:755	arg1	ejection					715:722	DNA ejection	711:722	DNA ejection	711:722	In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620.					
29843473	4	26	attach	linked	550:555	arg1	changes					560:566	changes	560:566	changes in host range or the occurrence of phage resistance	560:618	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	4	26	attach	linked	550:555	arg2	they					538:541	they	538:541	they	538:541	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	3	27	theme	environmental	389:401	arg1	conditions					403:412	the environmental conditions	385:412	the environmental conditions	385:412	However, O-antigen composition and structure can be highly variable depending on the environmental conditions.					
29843473	7	28	theme	low	1029:1031	arg1	temperatures					1033:1044	restrictive low temperatures	1017:1044	restrictive low temperatures	1017:1044	Moreover, we found that HK620 irreversibly adsorbed to the LPS receptor via its TSP at restrictive low temperatures, without opening the particle but could eject its DNA at permissive temperatures.					
29843473	0	29	theme	DNA	48:50	arg1	Release					52:58	Lipopolysaccharide-Mediated DNA Release	20:58	Lipopolysaccharide-Mediated DNA Release of Podovirus HK620	20:77	In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.					
29843473	8	30	theme	DNA	1263:1265	arg1	release					1267:1273	the DNA release	1259:1273	the DNA release from E. coli phage HK620	1259:1298	DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro.					
29843473	5	31	theme	DNA	711:713	arg1	ejection					715:722	DNA ejection	711:722	DNA ejection	711:722	In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620.					
29843473	0	32	theme	Lipopolysaccharide-Mediated	20:46	arg1	Release					52:58	Lipopolysaccharide-Mediated DNA Release	20:58	Lipopolysaccharide-Mediated DNA Release of Podovirus HK620	20:77	In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.					
29843473	2	33	theme	O-antigen-specific	183:200	arg1	bacteriophages					202:215	O-antigen-specific bacteriophages	183:215	O-antigen-specific bacteriophages	183:215	O-antigen-specific bacteriophages use tailspike proteins (TSP) to recognize and cleave the O-polysaccharide part of LPS.					
29843473	6	34	theme	Fluorescence-based	758:775	arg1	monitoring					777:786	Fluorescence-based monitoring	758:786	Fluorescence-based monitoring of DNA release	758:801	Fluorescence-based monitoring of DNA release showed that HK620 phage particles in vitro ejected their genome at velocities comparable to those found for other podoviruses.					
29843473	8	35	theme	O-antigen	1203:1211	arg1	composition					1184:1194	the composition	1180:1194	the composition of the O-antigen	1180:1211	DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro.					
29843473	5	36	dep	E.	733:734	arg1	coli					736:739	coli	736:739	coli	736:739	In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620.					
29843473	4	37	theme	early	481:485	arg1	steps					487:491	the early steps	477:491	the early steps of the bacteriophage infection cycle	477:528	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	0	38	theme	Release	52:58	arg1	Studies					9:15	In Vitro Studies	0:15	In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.	0:78	In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.					
29843473	9	39	theme	bacteriophage	1358:1370	arg1	mixtures					1372:1379	bacteriophage mixtures	1358:1379	bacteriophage mixtures	1358:1379	This finding can be significant when optimizing bacteriophage mixtures for therapy, where in natural environments O-antigen structures may rapidly change.					
29843473	1	40	theme	outermost	130:138	arg1	layer					140:144	an outermost layer	127:144	an outermost layer containing lipopolysaccharide (LPS)	127:180	Gram-negative bacteria protect themselves with an outermost layer containing lipopolysaccharide (LPS).					
29843473	7	41	theme	restrictive	1017:1027	arg1	temperatures					1033:1044	restrictive low temperatures	1017:1044	restrictive low temperatures	1017:1044	Moreover, we found that HK620 irreversibly adsorbed to the LPS receptor via its TSP at restrictive low temperatures, without opening the particle but could eject its DNA at permissive temperatures.					
29843473	8	42	theme	E.	1280:1281	arg1	HK620					1294:1298	E. coli phage HK620	1280:1298	E. coli phage HK620	1280:1298	DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro.					
29843473	3	43	theme	O-antigen	313:321	arg1	composition					323:333	O-antigen composition	313:333	O-antigen composition	313:333	However, O-antigen composition and structure can be highly variable depending on the environmental conditions.					
29843473	2	44	theme	O-polysaccharide	274:289	arg1	part					291:294	the O-polysaccharide part	270:294	the O-polysaccharide part of LPS	270:301	O-antigen-specific bacteriophages use tailspike proteins (TSP) to recognize and cleave the O-polysaccharide part of LPS.					
29843473	7	45	theme	LPS	989:991	arg1	receptor					993:1000	the LPS receptor	985:1000	the LPS receptor	985:1000	Moreover, we found that HK620 irreversibly adsorbed to the LPS receptor via its TSP at restrictive low temperatures, without opening the particle but could eject its DNA at permissive temperatures.					
29843473	4	46	theme	resistance	609:618	arg1	range					576:580	host range	571:580	host range	571:580	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	4	46	theme	resistance	609:618	arg1	occurrence					589:598	the occurrence	585:598	the occurrence of phage resistance	585:618	It is important to understand how these changes may influence the early steps of the bacteriophage infection cycle because they can be linked to changes in host range or the occurrence of phage resistance.					
29843473	5	47	theme	podovirus	741:749	arg1	HK620					751:755	the E. coli podovirus HK620	729:755	the E. coli podovirus HK620	729:755	In this work, we have analyzed how LPS preparations in vitro trigger particle opening and DNA ejection from the E. coli podovirus HK620.					
29843473	2	48	theme	tailspike	221:229	arg1	proteins					231:238	tailspike proteins	221:238	tailspike proteins (TSP)	221:244	O-antigen-specific bacteriophages use tailspike proteins (TSP) to recognize and cleave the O-polysaccharide part of LPS.					
29843473	2	48	theme	tailspike	221:229	arg1	TSP					241:243	TSP	241:243	TSP	241:243	O-antigen-specific bacteriophages use tailspike proteins (TSP) to recognize and cleave the O-polysaccharide part of LPS.					
29843473	8	49	theme	phage	1288:1292	arg1	HK620					1294:1298	E. coli phage HK620	1280:1298	E. coli phage HK620	1280:1298	DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro.					
29843473	7	50	from	temperatures	1033:1044	arg1	TSP					1010:1012	its TSP	1006:1012	its TSP at restrictive low temperatures	1006:1044	Moreover, we found that HK620 irreversibly adsorbed to the LPS receptor via its TSP at restrictive low temperatures, without opening the particle but could eject its DNA at permissive temperatures.					
29843473	0	51	theme	Podovirus	63:71	arg1	Release					52:58	Lipopolysaccharide-Mediated DNA Release	20:58	Lipopolysaccharide-Mediated DNA Release of Podovirus HK620	20:77	In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.					
29843473	1	52	contain	containing	146:155	arg1	layer					140:144	an outermost layer	127:144	an outermost layer containing lipopolysaccharide (LPS)	127:180	Gram-negative bacteria protect themselves with an outermost layer containing lipopolysaccharide (LPS).					
29843473	1	52	contain	containing	146:155	arg2	LPS					177:179	LPS	177:179	LPS	177:179	Gram-negative bacteria protect themselves with an outermost layer containing lipopolysaccharide (LPS).					
29843473	1	52	contain	containing	146:155	arg2	lipopolysaccharide					157:174	lipopolysaccharide	157:174	lipopolysaccharide (LPS)	157:180	Gram-negative bacteria protect themselves with an outermost layer containing lipopolysaccharide (LPS).					
29843473	0	53	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Studies of Lipopolysaccharide-Mediated DNA Release of Podovirus HK620.					
29843473	8	54	theme	coli	1283:1286	arg1	HK620					1294:1298	E. coli phage HK620	1280:1298	E. coli phage HK620	1280:1298	DNA ejection was solely stimulated by LPS, however, the composition of the O-antigen dictated whether the LPS receptor could start the DNA release from E. coli phage HK620 in vitro.					
30321293	3	0	theme	natural	656:662	arg1	rhythms					670:676	natural daily rhythms	656:676	natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are	656:740	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	1	1	theme	behavioral	195:204	arg1	avoidance					206:214	behavioral avoidance	195:214	behavioral avoidance of contagious individuals	195:240	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	1	theme	behavioral	195:204	arg1	strategy					266:273	an adaptive strategy	254:273	an adaptive strategy	254:273	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	2	2	theme	axillary	423:430	arg1	odor					432:435	human axillary odor	417:435	human axillary odor	417:435	Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicated that human axillary odor also becomes more aversive as a function of immune activation.					
30321293	2	3	theme	olfactory	367:375	arg1	cues					377:380	olfactory cues	367:380	olfactory cues	367:380	Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicated that human axillary odor also becomes more aversive as a function of immune activation.					
30321293	1	4	theme	relying	126:132	arg1	cost					118:121	the cost	114:121	the cost of relying on the immune system to defeat pathogens	114:173	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	4	theme	relying	126:132	arg1	high					178:181	high	178:181	high	178:181	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	5	from	relying	126:132	arg1	system					148:153	the immune system	137:153	the immune system	137:153	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	4	6	theme	human	802:806	arg1	urine					808:812	healthy human urine	794:812	healthy human urine	794:812	Whereas healthy human urine decreases in averseness over the course of a single day, inflammation interrupts this process and results in an increased urine odor averseness and an altered volatile composition.					
30321293	3	7	with	participants	527:538	arg1	weight					580:585	0.6 ng/kg body weight	565:585	0.6 ng/kg body weight	565:585	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	3	7	with	participants	527:538	arg1	lipopolysaccharide					545:562	lipopolysaccharide	545:562	lipopolysaccharide (0.6 ng/kg body weight)	545:586	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	1	8	theme	contagious	219:228	arg1	individuals					230:240	contagious individuals	219:240	contagious individuals	219:240	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	3	9	theme	urine	681:685	arg1	odor-its					687:694	urine odor-its	681:694	urine odor-its	681:694	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	1	10	from	cost	118:121	arg1	system					148:153	the immune system	137:153	the immune system	137:153	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	11	theme	Contagious	60:69	arg1	disease					71:77	Contagious disease	60:77	Contagious disease	60:77	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	11	theme	Contagious	60:69	arg1	threat					90:95	a major threat	82:95	a major threat to survival	82:107	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	0	12	from	Cues	39:42	arg1	Urine					53:57	Human Urine	47:57	Human Urine	47:57	Detection of Inflammation via Volatile Cues in Human Urine.					
30321293	2	13	theme	recent	389:394	arg1	study					396:400	a recent study	387:400	a recent study	387:400	Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicated that human axillary odor also becomes more aversive as a function of immune activation.					
30321293	4	14	theme	odor	942:945	arg1	averseness					947:956	an increased urine odor averseness	923:956	an increased urine odor averseness	923:956	Whereas healthy human urine decreases in averseness over the course of a single day, inflammation interrupts this process and results in an increased urine odor averseness and an altered volatile composition.					
30321293	5	15	from	system	1129:1134	arg1	role					1143:1146	its role	1139:1146	its role of keeping us alive and healthy	1139:1178	These results support the notion that subtle and early cues of sickness may be detected and avoided, thereby complementing the immune system in its role of keeping us alive and healthy.					
30321293	3	16	theme	dimensions	706:715	arg1	rhythms					670:676	natural daily rhythms	656:676	natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are	656:740	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	2	17	theme	Animal	276:281	arg1	findings					283:290	Animal findings	276:290	Animal findings	276:290	Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicated that human axillary odor also becomes more aversive as a function of immune activation.					
30321293	5	18	theme	sickness	1058:1065	arg1	cues					1050:1053	subtle and early cues	1033:1053	subtle and early cues of sickness	1033:1065	These results support the notion that subtle and early cues of sickness may be detected and avoided, thereby complementing the immune system in its role of keeping us alive and healthy.					
30321293	1	19	theme	immune	141:146	arg1	system					148:153	the immune system	137:153	the immune system	137:153	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	20	theme	individuals	230:240	arg1	avoidance					206:214	behavioral avoidance	195:214	behavioral avoidance of contagious individuals	195:240	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	20	theme	individuals	230:240	arg1	strategy					266:273	an adaptive strategy	254:273	an adaptive strategy	254:273	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	3	21	theme	healthy	513:519	arg1	participants					527:538	healthy human participants	513:538	healthy human participants with lipopolysaccharide (0.6 ng/kg body weight)	513:586	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	2	22	theme	sick	336:339	arg1	individuals					341:351	sick individuals	336:351	sick individuals	336:351	Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicated that human axillary odor also becomes more aversive as a function of immune activation.					
30321293	2	23	theme	activation	488:497	arg1	function					469:476	a function	467:476	a function of immune activation	467:497	Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicated that human axillary odor also becomes more aversive as a function of immune activation.					
30321293	1	24	theme	major	84:88	arg1	disease					71:77	Contagious disease	60:77	Contagious disease	60:77	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	24	theme	major	84:88	arg1	threat					90:95	a major threat	82:95	a major threat to survival	82:107	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	0	25	theme	Inflammation	13:24	arg1	Detection					0:8	Detection	0:8	Detection of Inflammation via Volatile Cues in Human Urine.	0:58	Detection of Inflammation via Volatile Cues in Human Urine.					
30321293	2	26	theme	immune	481:486	arg1	activation					488:497	immune activation	481:497	immune activation	481:497	Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicated that human axillary odor also becomes more aversive as a function of immune activation.					
30321293	5	27	theme	subtle	1033:1038	arg1	cues					1050:1053	subtle and early cues	1033:1053	subtle and early cues of sickness	1033:1065	These results support the notion that subtle and early cues of sickness may be detected and avoided, thereby complementing the immune system in its role of keeping us alive and healthy.					
30321293	1	28	theme	defeat	158:163	arg1	pathogens					165:173	defeat pathogens	158:173	defeat pathogens	158:173	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	2	29	theme	human	417:421	arg1	odor					432:435	human axillary odor	417:435	human axillary odor	417:435	Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicated that human axillary odor also becomes more aversive as a function of immune activation.					
30321293	3	30	theme	perceived	696:704	arg1	dimensions					706:715	urine odor-its perceived dimensions	681:715	urine odor-its perceived dimensions	681:715	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	0	31	theme	Volatile	30:37	arg1	Cues					39:42	Volatile Cues	30:42	Volatile Cues in Human Urine	30:57	Detection of Inflammation via Volatile Cues in Human Urine.					
30321293	1	32	theme	adaptive	257:264	arg1	strategy					266:273	an adaptive strategy	254:273	an adaptive strategy	254:273	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	32	theme	adaptive	257:264	arg1	avoidance					206:214	behavioral avoidance	195:214	behavioral avoidance of contagious individuals	195:240	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	5	33	theme	early	1044:1048	arg1	cues					1050:1053	subtle and early cues	1033:1053	subtle and early cues of sickness	1033:1065	These results support the notion that subtle and early cues of sickness may be detected and avoided, thereby complementing the immune system in its role of keeping us alive and healthy.					
30321293	5	34	theme	immune	1122:1127	arg1	system					1129:1134	the immune system	1118:1134	the immune system in its role of keeping us alive and healthy	1118:1178	These results support the notion that subtle and early cues of sickness may be detected and avoided, thereby complementing the immune system in its role of keeping us alive and healthy.					
30321293	0	35	theme	Human	47:51	arg1	Urine					53:57	Human Urine	47:57	Human Urine	47:57	Detection of Inflammation via Volatile Cues in Human Urine.					
30321293	4	36	theme	single	859:864	arg1	day					866:868	a single day	857:868	a single day	857:868	Whereas healthy human urine decreases in averseness over the course of a single day, inflammation interrupts this process and results in an increased urine odor averseness and an altered volatile composition.					
30321293	4	37	theme	increased	926:934	arg1	averseness					947:956	an increased urine odor averseness	923:956	an increased urine odor averseness	923:956	Whereas healthy human urine decreases in averseness over the course of a single day, inflammation interrupts this process and results in an increased urine odor averseness and an altered volatile composition.					
30321293	3	38	theme	human	521:525	arg1	participants					527:538	healthy human participants	513:538	healthy human participants with lipopolysaccharide (0.6 ng/kg body weight)	513:586	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	3	39	theme	daily	664:668	arg1	rhythms					670:676	natural daily rhythms	656:676	natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are	656:740	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	4	40	theme	altered	965:971	arg1	composition					982:992	an altered volatile composition	962:992	an altered volatile composition	962:992	Whereas healthy human urine decreases in averseness over the course of a single day, inflammation interrupts this process and results in an increased urine odor averseness and an altered volatile composition.					
30321293	3	41	theme	volatile	721:728	arg1	profile-are					730:740	volatile profile-are	721:740	volatile profile-are	721:740	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	4	42	theme	healthy	794:800	arg1	urine					808:812	healthy human urine	794:812	healthy human urine	794:812	Whereas healthy human urine decreases in averseness over the course of a single day, inflammation interrupts this process and results in an increased urine odor averseness and an altered volatile composition.					
30321293	3	43	dep	perceived	696:704	arg1	odor-its					687:694	urine odor-its	681:694	urine odor-its	681:694	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	3	44	theme	profile-are	730:740	arg1	rhythms					670:676	natural daily rhythms	656:676	natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are	656:740	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	3	45	theme	ng/kg	569:573	arg1	weight					580:585	0.6 ng/kg body weight	565:585	0.6 ng/kg body weight	565:585	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	3	45	theme	ng/kg	569:573	arg1	lipopolysaccharide					545:562	lipopolysaccharide	545:562	lipopolysaccharide (0.6 ng/kg body weight)	545:586	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	3	46	theme	inflammation	766:777	arg1	onset					779:783	inflammation onset	766:783	inflammation onset	766:783	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	4	47	theme	day	866:868	arg1	course					847:852	the course	843:852	the course of a single day	843:868	Whereas healthy human urine decreases in averseness over the course of a single day, inflammation interrupts this process and results in an increased urine odor averseness and an altered volatile composition.					
30321293	3	48	theme	body	575:578	arg1	weight					580:585	0.6 ng/kg body weight	565:585	0.6 ng/kg body weight	565:585	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	3	48	theme	body	575:578	arg1	lipopolysaccharide					545:562	lipopolysaccharide	545:562	lipopolysaccharide (0.6 ng/kg body weight)	545:586	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	3	49	theme	onset	779:783	arg1	hours					757:761	hours	757:761	hours of inflammation onset	757:783	By injecting healthy human participants with lipopolysaccharide (0.6 ng/kg body weight) to experimentally induce inflammation, this study demonstrates that natural daily rhythms of urine odor-its perceived dimensions and volatile profile-are altered within hours of inflammation onset.					
30321293	4	50	theme	urine	936:940	arg1	averseness					947:956	an increased urine odor averseness	923:956	an increased urine odor averseness	923:956	Whereas healthy human urine decreases in averseness over the course of a single day, inflammation interrupts this process and results in an increased urine odor averseness and an altered volatile composition.					
30321293	2	51	dep	the	356:358	arg1	aid					360:362	aid	360:362	aid	360:362	Animal findings demonstrate the ability to detect and avoid sick individuals by the aid of olfactory cues, and a recent study indicated that human axillary odor also becomes more aversive as a function of immune activation.					
30321293	1	52	from	system	148:153	arg1	cost					118:121	the cost	114:121	the cost of relying on the immune system to defeat pathogens	114:173	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	1	52	from	system	148:153	arg1	high					178:181	high	178:181	high	178:181	Contagious disease is a major threat to survival, and the cost of relying on the immune system to defeat pathogens is high; therefore, behavioral avoidance of contagious individuals is arguably an adaptive strategy.					
30321293	4	53	theme	volatile	973:980	arg1	composition					982:992	an altered volatile composition	962:992	an altered volatile composition	962:992	Whereas healthy human urine decreases in averseness over the course of a single day, inflammation interrupts this process and results in an increased urine odor averseness and an altered volatile composition.					
31389409	8	0	theme	microscopy	1254:1263	arg1	results					1271:1277	Transmission electron microscopy (TEM) results	1232:1277	Transmission electron microscopy (TEM) results	1232:1277	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	2	1	with	complexation	370:381	arg1	organization					398:409	nanoscale organization	388:409	nanoscale organization	388:409	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	2	2	theme	proteoglycans	345:357	arg1	complexation					370:381	the proteoglycans'/proteins' complexation	341:381	the proteoglycans'/proteins' complexation with nanoscale organization	341:409	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	10	3	dep	marrow	1661:1666	arg1	HS-5					1683:1686	HS-5	1683:1686	HS-5	1683:1686	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	10	3	dep	marrow	1661:1666	arg1	cells					1676:1680	stromal cells	1668:1680	human bone marrow stromal cells (HS-5)	1650:1687	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	12	4	theme	mitogenic	2002:2010	arg1	characteristics					2012:2026	the metabolic and mitogenic characteristics	1984:2026	the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs)	1984:2077	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	7	5	dep	Fourier	1103:1109	arg1	transform					1111:1119	transform	1111:1119	transform infrared spectroscopy (FTIR) analysis	1111:1157	Fourier transform infrared spectroscopy (FTIR) analysis indicated that complexation occurred through electrostatic interactions.					
31389409	3	6	theme	tissues	580:586	arg1	growth					454:459	growth	454:459	growth	454:459	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	3	6	theme	tissues	580:586	arg1	properties					562:571	mechanical properties	551:571	mechanical properties	551:571	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	3	6	theme	tissues	580:586	arg1	differentiation					509:523	cell differentiation	504:523	cell differentiation	504:523	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	3	6	theme	tissues	580:586	arg1	signaling					536:544	cell-cell signaling	526:544	cell-cell signaling	526:544	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	13	7	theme	regeneration	2190:2201	arg1	applications					2203:2214	tissue regeneration applications	2183:2214	tissue regeneration applications	2183:2214	Our results demonstrated the potential applications of these nanoparticles in therapeutic delivery for tissue regeneration applications.					
31389409	8	8	theme	±	1329:1329	arg1	nm					1334:1335	11 nm	1331:1335	11 nm	1331:1335	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	5	9	theme	chemical	901:908	arg1	composition					910:920	chemical composition	901:920	chemical composition	901:920	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	12	10	theme	vein	2047:2050	arg1	HUVECs					2071:2076	HUVECs	2071:2076	HUVECs	2071:2076	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	12	10	theme	vein	2047:2050	arg1	cells					2064:2068	human umbilical vein endothelial cells	2031:2068	human umbilical vein endothelial cells (HUVECs)	2031:2077	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	8	11	theme	nm	1334:1335	arg1	diameter					1314:1321	a diameter	1312:1321	a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively	1312:1472	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	2	12	theme	proteoglycans	292:304	arg1	chains					282:287	glycosaminoglycan (GAG) polymer chains	250:287	glycosaminoglycan (GAG) polymer chains of proteoglycans	250:304	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	9	13	theme	negative	1528:1535	arg1	mV					1565:1566	i.e.-25 ± 4 mV	1553:1566	i.e.-25 ± 4 mV for DS-PLL	1553:1577	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	9	13	theme	negative	1528:1535	arg1	potential					1542:1550	their negative zeta potential	1522:1550	their negative zeta potential	1522:1550	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	9	13	theme	negative	1528:1535	arg1	mV					1593:1594	-18 ± 3.5 mV	1583:1594	-18 ± 3.5 mV	1583:1594	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	12	14	theme	metabolic	1988:1996	arg1	characteristics					2012:2026	the metabolic and mitogenic characteristics	1984:2026	the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs)	1984:2077	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	8	15	theme	91	1341:1342	arg1	±					1344:1344	±	1344:1344	±	1344:1344	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	2	16	theme	chains	282:287	arg1	residual					238:245	residual	238:245	residual	238:245	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	8	17	theme	33	1346:1347	arg1	±					1344:1344	±	1344:1344	±	1344:1344	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	10	18	theme	stromal	1668:1674	arg1	HS-5					1683:1686	HS-5	1683:1686	HS-5	1683:1686	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	10	18	theme	stromal	1668:1674	arg1	cells					1676:1680	stromal cells	1668:1680	human bone marrow stromal cells (HS-5)	1650:1687	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	4	19	theme	controlled	746:755	arg1	release					757:763	controlled release	746:763	controlled release	746:763	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	10	20	theme	bone	1656:1659	arg1	marrow					1661:1666	human bone marrow stromal cells (HS-5)	1650:1687	human bone marrow stromal cells (HS-5)	1650:1687	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	2	21	theme	native	209:214	arg1	tissues					216:222	native tissues	209:222	native tissues	209:222	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	5	22	theme	proteoglycans	959:971	arg1	immobilization					941:954	growth factor immobilization	927:954	growth factor immobilization of proteoglycans	927:971	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	5	22	theme	proteoglycans	959:971	arg1	dimensions					889:898	the dimensions	885:898	the dimensions	885:898	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	5	22	theme	proteoglycans	959:971	arg1	composition					910:920	chemical composition	901:920	chemical composition	901:920	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	8	23	theme	sulfate	1365:1371	arg1	salt-poly-l-lysine					1380:1397	dermatan sulfate sodium salt-poly-l-lysine	1356:1397	dermatan sulfate sodium salt-poly-l-lysine (DS-PLL)	1356:1406	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	8	23	theme	sulfate	1365:1371	arg1	DS-PLL					1400:1405	DS-PLL	1400:1405	DS-PLL	1400:1405	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	11	24	theme	endothelial	1787:1797	arg1	VEGF					1814:1817	VEGF	1814:1817	VEGF	1814:1817	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	11	24	theme	endothelial	1787:1797	arg1	factor					1806:1811	vascular endothelial growth factor	1778:1811	vascular endothelial growth factor (VEGF)	1778:1818	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	5	25	theme	native	976:981	arg1	tissues					983:989	native tissues	976:989	native tissues	976:989	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	6	26	theme	physicochemical	1075:1089	arg1	properties					1091:1100	physicochemical properties	1075:1100	physicochemical properties	1075:1100	PCNs were prepared by a polymer-polymer pair reaction method and characterized for physicochemical properties.					
31389409	5	27	theme	polyelectrolyte	797:811	arg1	PCNs					836:839	PCNs	836:839	PCNs	836:839	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	5	27	theme	polyelectrolyte	797:811	arg1	nanoparticles					821:833	polyelectrolyte complex nanoparticles	797:833	polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues	797:989	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	5	28	theme	growth	927:932	arg1	immobilization					941:954	growth factor immobilization	927:954	growth factor immobilization of proteoglycans	927:971	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	9	29	theme	±	1587:1587	arg1	potential					1542:1550	their negative zeta potential	1522:1550	their negative zeta potential	1522:1550	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	9	29	theme	±	1587:1587	arg1	mV					1593:1594	-18 ± 3.5 mV	1583:1594	-18 ± 3.5 mV	1583:1594	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	6	30	theme	pair	1032:1035	arg1	method					1046:1051	a polymer-polymer pair reaction method	1014:1051	a polymer-polymer pair reaction method	1014:1051	PCNs were prepared by a polymer-polymer pair reaction method and characterized for physicochemical properties.					
31389409	8	31	theme	tragacanth-poly-l-lysine	1416:1439	arg1	complexes					1450:1458	tragacanth-poly-l-lysine (GT-PLL) complexes	1416:1458	tragacanth-poly-l-lysine (GT-PLL) complexes	1416:1458	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	12	32	theme	PCNs	1908:1911	arg1	capability					1894:1903	The capability	1890:1903	The capability of PCNs to preserve VEGF activity for up to 21 days	1890:1955	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	4	33	theme	factors	775:781	arg1	immobilization					727:740	immobilization	727:740	immobilization	727:740	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	4	33	theme	factors	775:781	arg1	release					757:763	controlled release	746:763	controlled release	746:763	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	0	34	theme	Biomimetic	0:9	arg1	proteoglycan					11:22	Biomimetic proteoglycan	0:22	Biomimetic proteoglycan	0:22	Biomimetic proteoglycan nanoparticles for growth factor immobilization and delivery.					
31389409	1	35	theme	growth	101:106	arg1	factors					108:114	growth factors	101:114	growth factors	101:114	The delivery of growth factors is often challenging due to their short half-life, low stability, and rapid deactivation.					
31389409	10	36	theme	live/dead	1713:1721	arg1	assay					1723:1727	a live/dead assay	1711:1727	a live/dead assay	1711:1727	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	3	37	theme	biological	418:427	arg1	assemblies					429:438	These biological assemblies	412:438	These biological assemblies	412:438	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	3	38	theme	surface	473:479	arg1	interactions					490:501	factor-cell surface receptor interactions	461:501	factor-cell surface receptor interactions	461:501	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	0	39	theme	growth	42:47	arg1	factor					49:54	growth factor immobilization and delivery	42:82	growth factor immobilization and delivery	42:82	Biomimetic proteoglycan nanoparticles for growth factor immobilization and delivery.					
31389409	10	40	with	contact	1637:1643	arg1	marrow					1661:1666	human bone marrow stromal cells (HS-5)	1650:1687	human bone marrow stromal cells (HS-5)	1650:1687	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	10	41	theme	activity	1743:1750	arg1	measurement					1752:1762	metabolic activity measurement	1733:1762	metabolic activity measurement	1733:1762	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	10	42	from	Cytocompatibility	1608:1624	arg1	contact					1637:1643	contact	1637:1643	contact with human bone marrow stromal cells (HS-5)	1637:1687	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	3	43	theme	cell	504:507	arg1	differentiation					509:523	cell differentiation	504:523	cell differentiation	504:523	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	1	44	theme	short	150:154	arg1	half-life					156:164	their short half-life	144:164	their short half-life	144:164	The delivery of growth factors is often challenging due to their short half-life, low stability, and rapid deactivation.					
31389409	13	45	from	applications	2119:2130	arg1	delivery					2170:2177	therapeutic delivery	2158:2177	therapeutic delivery for tissue regeneration applications	2158:2214	Our results demonstrated the potential applications of these nanoparticles in therapeutic delivery for tissue regeneration applications.					
31389409	12	46	theme	endothelial	2052:2062	arg1	HUVECs					2071:2076	HUVECs	2071:2076	HUVECs	2071:2076	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	12	46	theme	endothelial	2052:2062	arg1	cells					2064:2068	human umbilical vein endothelial cells	2031:2068	human umbilical vein endothelial cells (HUVECs)	2031:2077	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	12	47	theme	human	2031:2035	arg1	HUVECs					2071:2076	HUVECs	2071:2076	HUVECs	2071:2076	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	12	47	theme	human	2031:2035	arg1	cells					2064:2068	human umbilical vein endothelial cells	2031:2068	human umbilical vein endothelial cells (HUVECs)	2031:2077	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	4	48	theme	novel	638:642	arg1	nanocarriers					668:679	novel biomimetic proteoglycan nanocarriers	638:679	novel biomimetic proteoglycan nanocarriers	638:679	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	7	49	theme	electrostatic	1204:1216	arg1	interactions					1218:1229	electrostatic interactions	1204:1229	electrostatic interactions	1204:1229	Fourier transform infrared spectroscopy (FTIR) analysis indicated that complexation occurred through electrostatic interactions.					
31389409	9	50	theme	colloidal	1479:1487	arg1	nanoparticles					1489:1501	The colloidal nanoparticles	1475:1501	The colloidal nanoparticles	1475:1501	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	9	50	theme	colloidal	1479:1487	arg1	stable					1508:1513	stable	1508:1513	stable	1508:1513	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	8	51	theme	electron	1245:1252	arg1	TEM					1266:1268	TEM	1266:1268	TEM	1266:1268	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	8	51	theme	electron	1245:1252	arg1	microscopy					1254:1263	Transmission electron microscopy	1232:1263	Transmission electron microscopy (TEM) results	1232:1277	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	10	52	theme	PCNs	1629:1632	arg1	Cytocompatibility					1608:1624	Cytocompatibility	1608:1624	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5)	1608:1687	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	0	53	dep	factor	49:54	arg1	delivery					75:82	delivery	75:82	delivery	75:82	Biomimetic proteoglycan nanoparticles for growth factor immobilization and delivery.					
31389409	0	53	dep	factor	49:54	arg1	immobilization					56:69	immobilization	56:69	immobilization	56:69	Biomimetic proteoglycan nanoparticles for growth factor immobilization and delivery.					
31389409	2	54	theme	polymer	274:280	arg1	chains					282:287	glycosaminoglycan (GAG) polymer chains	250:287	glycosaminoglycan (GAG) polymer chains of proteoglycans	250:304	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	13	55	theme	tissue	2183:2188	arg1	applications					2203:2214	tissue regeneration applications	2183:2214	tissue regeneration applications	2183:2214	Our results demonstrated the potential applications of these nanoparticles in therapeutic delivery for tissue regeneration applications.					
31389409	5	56	dep	factor	852:857	arg1	nanocarriers					859:870	nanocarriers	859:870	growth factor nanocarriers	845:870	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	4	57	theme	facile	610:615	arg1	procedure					617:625	a facile procedure	608:625	a facile procedure to prepare novel biomimetic proteoglycan nanocarriers	608:679	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	7	58	dep	transform	1111:1119	arg1	infrared					1121:1128	infrared	1121:1128	transform infrared spectroscopy (FTIR) analysis	1111:1157	Fourier transform infrared spectroscopy (FTIR) analysis indicated that complexation occurred through electrostatic interactions.					
31389409	11	59	theme	growth	1874:1879	arg1	factors					1881:1887	growth factors	1874:1887	growth factors	1874:1887	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	1	60	theme	low	167:169	arg1	stability					171:179	low stability	167:179	low stability	167:179	The delivery of growth factors is often challenging due to their short half-life, low stability, and rapid deactivation.					
31389409	8	61	theme	60	1326:1327	arg1	±					1329:1329	±	1329:1329	±	1329:1329	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	10	62	theme	human	1650:1654	arg1	marrow					1661:1666	human bone marrow stromal cells (HS-5)	1650:1687	human bone marrow stromal cells (HS-5)	1650:1687	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	6	63	theme	polymer-polymer	1016:1030	arg1	method					1046:1051	a polymer-polymer pair reaction method	1014:1051	a polymer-polymer pair reaction method	1014:1051	PCNs were prepared by a polymer-polymer pair reaction method and characterized for physicochemical properties.					
31389409	4	64	theme	derived	701:707	arg1	polymers					709:716	naturally derived polymers	691:716	naturally derived polymers	691:716	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	9	65	theme	zeta	1537:1540	arg1	mV					1565:1566	i.e.-25 ± 4 mV	1553:1566	i.e.-25 ± 4 mV for DS-PLL	1553:1577	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	9	65	theme	zeta	1537:1540	arg1	potential					1542:1550	their negative zeta potential	1522:1550	their negative zeta potential	1522:1550	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	9	65	theme	zeta	1537:1540	arg1	mV					1593:1594	-18 ± 3.5 mV	1583:1594	-18 ± 3.5 mV	1583:1594	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	4	66	theme	proteoglycan	655:666	arg1	nanocarriers					668:679	novel biomimetic proteoglycan nanocarriers	638:679	novel biomimetic proteoglycan nanocarriers	638:679	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	10	67	theme	metabolic	1733:1741	arg1	measurement					1752:1762	metabolic activity measurement	1733:1762	metabolic activity measurement	1733:1762	Cytocompatibility of PCNs in contact with human bone marrow stromal cells (HS-5) was confirmed through a live/dead assay and metabolic activity measurement.					
31389409	7	68	theme	spectroscopy	1130:1141	arg1	analysis					1150:1157	spectroscopy (FTIR) analysis	1130:1157	spectroscopy (FTIR) analysis	1130:1157	Fourier transform infrared spectroscopy (FTIR) analysis indicated that complexation occurred through electrostatic interactions.					
31389409	13	69	theme	nanoparticles	2141:2153	arg1	applications					2119:2130	the potential applications	2105:2130	the potential applications of these nanoparticles in therapeutic delivery for tissue regeneration applications	2105:2214	Our results demonstrated the potential applications of these nanoparticles in therapeutic delivery for tissue regeneration applications.					
31389409	4	70	link	derived	701:707	arg1	polymers					709:716	naturally derived polymers	691:716	naturally derived polymers	691:716	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	11	71	theme	PCNs	1856:1859	arg1	ability					1845:1851	the ability	1841:1851	the ability of PCNs to stabilize growth factors	1841:1887	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	13	72	theme	therapeutic	2158:2168	arg1	delivery					2170:2177	therapeutic delivery	2158:2177	therapeutic delivery for tissue regeneration applications	2158:2214	Our results demonstrated the potential applications of these nanoparticles in therapeutic delivery for tissue regeneration applications.					
31389409	8	73	contain	had	1308:1310	arg2	diameter					1314:1321	a diameter	1312:1321	a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively	1312:1472	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	8	73	contain	had	1308:1310	arg1	nanocarriers					1295:1306	the nanocarriers	1291:1306	the nanocarriers	1291:1306	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	9	74	theme	±	1561:1561	arg1	mV					1565:1566	i.e.-25 ± 4 mV	1553:1566	i.e.-25 ± 4 mV for DS-PLL	1553:1577	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	9	74	theme	±	1561:1561	arg1	potential					1542:1550	their negative zeta potential	1522:1550	their negative zeta potential	1522:1550	The colloidal nanoparticles were stable due to their negative zeta potential, i.e.-25 ± 4 mV for DS-PLL and -18 ± 3.5 mV for GT-PLL.					
31389409	8	75	theme	nm	1349:1350	arg1	diameter					1314:1321	a diameter	1312:1321	a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively	1312:1472	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	13	76	theme	potential	2109:2117	arg1	applications					2119:2130	the potential applications	2105:2130	the potential applications of these nanoparticles in therapeutic delivery for tissue regeneration applications	2105:2214	Our results demonstrated the potential applications of these nanoparticles in therapeutic delivery for tissue regeneration applications.					
31389409	2	77	theme	glycosaminoglycan	250:266	arg1	chains					282:287	glycosaminoglycan (GAG) polymer chains	250:287	glycosaminoglycan (GAG) polymer chains of proteoglycans	250:304	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	8	78	theme	dermatan	1356:1363	arg1	sulfate					1365:1371	dermatan sulfate	1356:1371	dermatan sulfate sodium salt-poly-l-lysine (DS-PLL)	1356:1406	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	11	79	theme	growth	1799:1804	arg1	VEGF					1814:1817	VEGF	1814:1817	VEGF	1814:1817	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	11	79	theme	growth	1799:1804	arg1	factor					1806:1811	vascular endothelial growth factor	1778:1811	vascular endothelial growth factor (VEGF)	1778:1818	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	1	80	theme	rapid	186:190	arg1	deactivation					192:203	rapid deactivation	186:203	rapid deactivation	186:203	The delivery of growth factors is often challenging due to their short half-life, low stability, and rapid deactivation.					
31389409	8	81	theme	sodium	1373:1378	arg1	salt-poly-l-lysine					1380:1397	dermatan sulfate sodium salt-poly-l-lysine	1356:1397	dermatan sulfate sodium salt-poly-l-lysine (DS-PLL)	1356:1406	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	8	81	theme	sodium	1373:1378	arg1	DS-PLL					1400:1405	DS-PLL	1400:1405	DS-PLL	1400:1405	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	11	82	theme	vascular	1778:1785	arg1	VEGF					1814:1817	VEGF	1814:1817	VEGF	1814:1817	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	11	82	theme	vascular	1778:1785	arg1	factor					1806:1811	vascular endothelial growth factor	1778:1811	vascular endothelial growth factor (VEGF)	1778:1818	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	5	83	theme	complex	813:819	arg1	PCNs					836:839	PCNs	836:839	PCNs	836:839	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	5	83	theme	complex	813:819	arg1	nanoparticles					821:833	polyelectrolyte complex nanoparticles	797:833	polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues	797:989	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	11	84	used	used	1824:1827	arg2	VEGF					1814:1817	VEGF	1814:1817	VEGF	1814:1817	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	11	84	used	used	1824:1827	arg2	factor					1806:1811	vascular endothelial growth factor	1778:1811	vascular endothelial growth factor (VEGF)	1778:1818	In addition, vascular endothelial growth factor (VEGF) was used to evaluate the ability of PCNs to stabilize growth factors.					
31389409	5	85	theme	factor	934:939	arg1	immobilization					941:954	growth factor immobilization	927:954	growth factor immobilization of proteoglycans	927:971	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	4	86	dep	immobilization	727:740	arg1	the					723:725	the	723:725	the	723:725	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	6	87	theme	reaction	1037:1044	arg1	method					1046:1051	a polymer-polymer pair reaction method	1014:1051	a polymer-polymer pair reaction method	1014:1051	PCNs were prepared by a polymer-polymer pair reaction method and characterized for physicochemical properties.					
31389409	12	88	dep	21	1949:1950	arg1	to					1946:1947	to	1946:1947	to	1946:1947	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	2	89	dep	proteoglycans	345:357	arg1	/proteins					359:367	/proteins	359:367	/proteins	359:367	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	2	90	theme	nanoscale	388:396	arg1	organization					398:409	nanoscale organization	388:409	nanoscale organization	388:409	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	5	91	theme	growth	845:850	arg1	factor					852:857	growth factor	845:857	growth factor nanocarriers	845:870	We developed polyelectrolyte complex nanoparticles (PCNs) as growth factor nanocarriers, which mimic the dimensions, chemical composition, and growth factor immobilization of proteoglycans in native tissues.					
31389409	12	92	theme	VEGF	1925:1928	arg1	activity					1930:1937	VEGF activity	1925:1937	VEGF activity	1925:1937	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	12	93	theme	umbilical	2037:2045	arg1	HUVECs					2071:2076	HUVECs	2071:2076	HUVECs	2071:2076	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	12	93	theme	umbilical	2037:2045	arg1	cells					2064:2068	human umbilical vein endothelial cells	2031:2068	human umbilical vein endothelial cells (HUVECs)	2031:2077	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	4	94	theme	growth	768:773	arg1	factors					775:781	growth factors	768:781	growth factors	768:781	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	1	95	theme	factors	108:114	arg1	delivery					89:96	The delivery	85:96	The delivery of growth factors	85:114	The delivery of growth factors is often challenging due to their short half-life, low stability, and rapid deactivation.					
31389409	8	96	theme	GT-PLL	1442:1447	arg1	complexes					1450:1458	tragacanth-poly-l-lysine (GT-PLL) complexes	1416:1458	tragacanth-poly-l-lysine (GT-PLL) complexes	1416:1458	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	12	97	theme	cells	2064:2068	arg1	characteristics					2012:2026	the metabolic and mitogenic characteristics	1984:2026	the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs)	1984:2077	The capability of PCNs to preserve VEGF activity for up to 21 days was confirmed by analyzing the metabolic and mitogenic characteristics of human umbilical vein endothelial cells (HUVECs).					
31389409	3	98	theme	receptor	481:488	arg1	interactions					490:501	factor-cell surface receptor interactions	461:501	factor-cell surface receptor interactions	461:501	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	3	99	dep	growth	454:459	arg1	interactions					490:501	factor-cell surface receptor interactions	461:501	factor-cell surface receptor interactions	461:501	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	3	100	theme	cell-cell	526:534	arg1	signaling					536:544	cell-cell signaling	526:544	cell-cell signaling	526:544	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	2	101	theme	growth	318:323	arg1	factors					325:331	growth factors	318:331	growth factors	318:331	In native tissues, the sulfated residual of glycosaminoglycan (GAG) polymer chains of proteoglycans immobilizes growth factors through the proteoglycans'/proteins' complexation with nanoscale organization.					
31389409	8	102	theme	Transmission	1232:1243	arg1	TEM					1266:1268	TEM	1266:1268	TEM	1266:1268	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	8	102	theme	Transmission	1232:1243	arg1	microscopy					1254:1263	Transmission electron microscopy	1232:1263	Transmission electron microscopy (TEM) results	1232:1277	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	8	103	dep	salt-poly-l-lysine	1380:1397	arg1	complexes					1450:1458	tragacanth-poly-l-lysine (GT-PLL) complexes	1416:1458	tragacanth-poly-l-lysine (GT-PLL) complexes	1416:1458	Transmission electron microscopy (TEM) results showed that the nanocarriers had a diameter of 60 ± 11 nm and 91 ± 33 nm for dermatan sulfate sodium salt-poly-l-lysine (DS-PLL) and gum tragacanth-poly-l-lysine (GT-PLL) complexes, respectively.					
31389409	3	104	theme	factor-cell	461:471	arg1	interactions					490:501	factor-cell surface receptor interactions	461:501	factor-cell surface receptor interactions	461:501	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
31389409	4	105	theme	biomimetic	644:653	arg1	nanocarriers					668:679	novel biomimetic proteoglycan nanocarriers	638:679	novel biomimetic proteoglycan nanocarriers	638:679	Here, we introduce a facile procedure to prepare novel biomimetic proteoglycan nanocarriers, based on naturally derived polymers, for the immobilization and controlled release of growth factors.					
31389409	3	106	theme	mechanical	551:560	arg1	properties					562:571	mechanical properties	551:571	mechanical properties	551:571	These biological assemblies can influence growth factor-cell surface receptor interactions, cell differentiation, cell-cell signaling, and mechanical properties of the tissues.					
30933483	3	0	from	effective	428:436	arg1	polarization					465:476	M1 polarization	462:476	M1 polarization	462:476	We report magnetite IONPs are more effective compared to hematite in M1 polarization and tumor suppression.					
30933483	3	0	from	effective	428:436	arg1	suppression					488:498	tumor suppression	482:498	tumor suppression	482:498	We report magnetite IONPs are more effective compared to hematite in M1 polarization and tumor suppression.					
30933483	3	1	dep	report	396:401	arg1	effective					428:436	effective	428:436	effective	428:436	We report magnetite IONPs are more effective compared to hematite in M1 polarization and tumor suppression.					
30933483	3	2	from	polarization	465:476	arg1	effective					428:436	effective	428:436	effective	428:436	We report magnetite IONPs are more effective compared to hematite in M1 polarization and tumor suppression.					
30933483	0	3	theme	Iron	13:16	arg1	Activation					43:52	Iron Oxide-Induced Macrophage Activation	13:52	Iron Oxide-Induced Macrophage Activation	13:52	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30933483	0	4	theme	Composition	69:79	arg1	Impact					59:64	The Impact	55:64	The Impact of Composition	55:79	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30933483	0	4	theme	Composition	69:79	arg1	Pathway					110:116	the Underlying Signaling Pathway	85:116	the Underlying Signaling Pathway	85:116	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30933483	4	5	theme	interferon	548:557	arg1	factor					570:575	interferon regulatory factor	548:575	interferon regulatory factor 5 signaling pathway	548:595	Moreover, magnetite IONPs specifically rely on interferon regulatory factor 5 signaling pathway for M1 polarization and down-regulate M2-assoicated arginase-1.					
30933483	4	6	theme	magnetite	511:519	arg1	IONPs					521:525	magnetite IONPs	511:525	magnetite IONPs	511:525	Moreover, magnetite IONPs specifically rely on interferon regulatory factor 5 signaling pathway for M1 polarization and down-regulate M2-assoicated arginase-1.					
30933483	0	7	theme	Underlying	89:98	arg1	Pathway					110:116	the Underlying Signaling Pathway	85:116	the Underlying Signaling Pathway	85:116	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30933483	1	8	theme	tumor-suppressing	273:289	arg1	M1					302:303	M1	302:303	M1	302:303	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	1	8	theme	tumor-suppressing	273:289	arg1	phenotype					291:299	tumor-suppressing phenotype	273:299	tumor-suppressing phenotype (M1)	273:304	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	1	9	contain	have	152:155	arg1	IONPs					145:149	IONPs	145:149	IONPs	145:149	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	1	9	contain	have	152:155	arg1	nanoparticles					130:142	Iron oxide nanoparticles	119:142	Iron oxide nanoparticles (IONPs)	119:150	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	1	9	contain	have	152:155	arg2	applications					177:188	emerging anticancer applications	157:188	emerging anticancer applications	157:188	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	4	10	theme	M2-assoicated	635:647	arg1	arginase-1					649:658	M2-assoicated arginase-1	635:658	M2-assoicated arginase-1	635:658	Moreover, magnetite IONPs specifically rely on interferon regulatory factor 5 signaling pathway for M1 polarization and down-regulate M2-assoicated arginase-1.					
30933483	5	11	theme	new	681:683	arg1	understandings					685:698	new understandings	681:698	new understandings	681:698	This study provides new understandings and paves the way for designing advanced iron-based anticancer technologies.					
30933483	1	12	theme	emerging	157:164	arg1	applications					177:188	emerging anticancer applications	157:188	emerging anticancer applications	157:188	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	0	13	theme	Signaling	100:108	arg1	Pathway					110:116	the Underlying Signaling Pathway	85:116	the Underlying Signaling Pathway	85:116	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30933483	1	14	theme	anticancer	166:175	arg1	applications					177:188	emerging anticancer applications	157:188	emerging anticancer applications	157:188	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	4	15	theme	M1	601:602	arg1	polarization					604:615	M1 polarization	601:615	M1 polarization	601:615	Moreover, magnetite IONPs specifically rely on interferon regulatory factor 5 signaling pathway for M1 polarization and down-regulate M2-assoicated arginase-1.					
30933483	2	16	theme	underlying	320:329	arg1	mechanism					331:339	the underlying mechanism	316:339	the underlying mechanism	316:339	However, the underlying mechanism and structure-function relationship remain unclear.					
30933483	0	17	theme	Oxide-Induced	18:30	arg1	Activation					43:52	Iron Oxide-Induced Macrophage Activation	13:52	Iron Oxide-Induced Macrophage Activation	13:52	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30933483	3	18	theme	magnetite	403:411	arg1	IONPs					413:417	magnetite IONPs	403:417	magnetite IONPs	403:417	We report magnetite IONPs are more effective compared to hematite in M1 polarization and tumor suppression.					
30933483	1	19	theme	tumor-associated	205:220	arg1	macrophages					222:232	tumor-associated macrophages	205:232	tumor-associated macrophages from tumor-promoting phenotype (M2)	205:268	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	0	20	theme	Activation	43:52	arg1	Mechanism					0:8	Mechanism	0:8	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.	0:117	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30933483	2	21	theme	structure-function	345:362	arg1	relationship					364:375	structure-function relationship	345:375	structure-function relationship	345:375	However, the underlying mechanism and structure-function relationship remain unclear.					
30933483	0	22	theme	Macrophage	32:41	arg1	Activation					43:52	Iron Oxide-Induced Macrophage Activation	13:52	Iron Oxide-Induced Macrophage Activation	13:52	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30933483	4	23	theme	factor	570:575	arg1	pathway					589:595	interferon regulatory factor 5 signaling pathway	548:595	interferon regulatory factor 5 signaling pathway	548:595	Moreover, magnetite IONPs specifically rely on interferon regulatory factor 5 signaling pathway for M1 polarization and down-regulate M2-assoicated arginase-1.					
30933483	3	24	theme	tumor	482:486	arg1	suppression					488:498	tumor suppression	482:498	tumor suppression	482:498	We report magnetite IONPs are more effective compared to hematite in M1 polarization and tumor suppression.					
30933483	4	25	theme	regulatory	559:568	arg1	factor					570:575	interferon regulatory factor	548:575	interferon regulatory factor 5 signaling pathway	548:595	Moreover, magnetite IONPs specifically rely on interferon regulatory factor 5 signaling pathway for M1 polarization and down-regulate M2-assoicated arginase-1.					
30933483	5	26	theme	advanced	732:739	arg1	technologies					763:774	advanced iron-based anticancer technologies	732:774	advanced iron-based anticancer technologies	732:774	This study provides new understandings and paves the way for designing advanced iron-based anticancer technologies.					
30933483	3	27	theme	M1	462:463	arg1	polarization					465:476	M1 polarization	462:476	M1 polarization	462:476	We report magnetite IONPs are more effective compared to hematite in M1 polarization and tumor suppression.					
30933483	4	28	theme	signaling	579:587	arg1	pathway					589:595	interferon regulatory factor 5 signaling pathway	548:595	interferon regulatory factor 5 signaling pathway	548:595	Moreover, magnetite IONPs specifically rely on interferon regulatory factor 5 signaling pathway for M1 polarization and down-regulate M2-assoicated arginase-1.					
30933483	5	29	theme	iron-based	741:750	arg1	technologies					763:774	advanced iron-based anticancer technologies	732:774	advanced iron-based anticancer technologies	732:774	This study provides new understandings and paves the way for designing advanced iron-based anticancer technologies.					
30933483	3	30	from	suppression	488:498	arg1	effective					428:436	effective	428:436	effective	428:436	We report magnetite IONPs are more effective compared to hematite in M1 polarization and tumor suppression.					
30933483	1	31	theme	Iron	119:122	arg1	oxide					124:128	Iron oxide	119:128	Iron oxide nanoparticles (IONPs)	119:150	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	5	32	theme	anticancer	752:761	arg1	technologies					763:774	advanced iron-based anticancer technologies	732:774	advanced iron-based anticancer technologies	732:774	This study provides new understandings and paves the way for designing advanced iron-based anticancer technologies.					
30933483	1	33	theme	oxide	124:128	arg1	IONPs					145:149	IONPs	145:149	IONPs	145:149	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	1	33	theme	oxide	124:128	arg1	nanoparticles					130:142	Iron oxide nanoparticles	119:142	Iron oxide nanoparticles (IONPs)	119:150	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	1	34	theme	tumor-promoting	239:253	arg1	M2					266:267	M2	266:267	M2	266:267	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	1	34	theme	tumor-promoting	239:253	arg1	phenotype					255:263	tumor-promoting phenotype	239:263	tumor-promoting phenotype (M2)	239:268	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	1	35	from	phenotype	255:263	arg1	macrophages					222:232	tumor-associated macrophages	205:232	tumor-associated macrophages from tumor-promoting phenotype (M2)	205:268	Iron oxide nanoparticles (IONPs) have emerging anticancer applications via polarizing tumor-associated macrophages from tumor-promoting phenotype (M2) to tumor-suppressing phenotype (M1).					
30933483	0	36	dep	Mechanism	0:8	arg1	Impact					59:64	The Impact	55:64	The Impact of Composition	55:79	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30933483	0	36	dep	Mechanism	0:8	arg1	Pathway					110:116	the Underlying Signaling Pathway	85:116	the Underlying Signaling Pathway	85:116	Mechanism of Iron Oxide-Induced Macrophage Activation: The Impact of Composition and the Underlying Signaling Pathway.					
30156533	3	0	theme	strain	183:188	arg1	nov					172:174	nov	172:174	nov	172:174	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	3	0	theme	strain	183:188	arg1	14696T					219:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	8	1	theme	1.4 	729:732	arg1	%					733:733	%	733:733	%	733:733	nov. differed by 1.4 % nucleotide substitutions from Su.					
30156533	9	2	theme	%	795:795	arg1	substitutions					808:820	1.9 % nucleotide substitutions	791:820	1.9 % nucleotide substitutions from Su	791:828	lignohabitans, and by 1.9 % nucleotide substitutions from Su.					
30156533	4	3	theme	Sugiyamaella	439:450	arg1	species					418:424	the species	414:424	the species of the genus Sugiyamaella	414:450	The novel species is able to assimilate cellobiose, salicin and d-xylose, which was typical of the species of the genus Sugiyamaella.					
30156533	3	4	theme	NYNU	190:193	arg1	nov					172:174	nov	172:174	nov	172:174	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	3	4	theme	NYNU	190:193	arg1	14696T					219:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	4	5	theme	genus	433:437	arg1	Sugiyamaella					439:450	the genus Sugiyamaella	429:450	the genus Sugiyamaella	429:450	The novel species is able to assimilate cellobiose, salicin and d-xylose, which was typical of the species of the genus Sugiyamaella.					
30156533	5	6	theme	internal	518:525	arg1	regions					546:552	internal transcribed spacer regions	518:552	internal transcribed spacer regions	518:552	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	13	7	from	species	985:991	arg1	substitutions					951:963	more than 6.5 % nucleotide substitutions	924:963	more than 6.5 % nucleotide substitutions from the latter two species	924:991	nov. displayed more than 6.5 % nucleotide substitutions from the latter two species, showing that it is a genetically separate species.					
30156533	8	8	theme	nucleotide	735:744	arg1	substitutions					746:758	1.4 % nucleotide substitutions	729:758	1.4 % nucleotide substitutions from Su	729:766	nov. differed by 1.4 % nucleotide substitutions from Su.					
30156533	3	9	theme	type	178:181	arg1	nov					172:174	nov	172:174	nov	172:174	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	3	9	theme	type	178:181	arg1	14696T					219:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	5	10	theme	transcribed	527:537	arg1	regions					546:552	internal transcribed spacer regions	518:552	internal transcribed spacer regions	518:552	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	1	11	theme	yeast	44:48	arg1	species					50:56	a yeast species	42:56	a yeast species isolated from rotting wood	42:83	nov., a yeast species isolated from rotting wood.					
30156533	13	12	theme	nucleotide	940:949	arg1	substitutions					951:963	more than 6.5 % nucleotide substitutions	924:963	more than 6.5 % nucleotide substitutions from the latter two species	924:991	nov. displayed more than 6.5 % nucleotide substitutions from the latter two species, showing that it is a genetically separate species.					
30156533	8	13	theme	%	733:733	arg1	substitutions					746:758	1.4 % nucleotide substitutions	729:758	1.4 % nucleotide substitutions from Su	729:766	nov. differed by 1.4 % nucleotide substitutions from Su.					
30156533	5	14	theme	closest	672:678	arg1	relatives					680:688	its closest relatives	668:688	its closest relatives	668:688	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	15	theme	regions	546:552	arg1	Analysis					453:460	Analysis	453:460	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains	453:569	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	16	theme	spacer	539:544	arg1	regions					546:552	internal transcribed spacer regions	518:552	internal transcribed spacer regions	518:552	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	10	17	dep	marionensis	831:841	arg1	sequences					856:864	the D1/D2 sequences	846:864	the D1/D2 sequences	846:864	marionensis in the D1/D2 sequences.					
30156533	10	18	theme	D1/D2	850:854	arg1	sequences					856:864	the D1/D2 sequences	846:864	the D1/D2 sequences	846:864	marionensis in the D1/D2 sequences.					
30156533	2	19	theme	yeast	121:125	arg1	f.a.					162:165	f.a.	162:165	f.a.	162:165	Three strains representing a novel yeast species, Sugiyamaella xiaguanensis f.a., sp.					
30156533	2	19	theme	yeast	121:125	arg1	species					127:133	a novel yeast species	113:133	a novel yeast species	113:133	Three strains representing a novel yeast species, Sugiyamaella xiaguanensis f.a., sp.					
30156533	5	20	theme	large	490:494	arg1	gene					509:512	the large subunit rRNA gene	486:512	the large subunit rRNA gene	486:512	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	2	21	theme	novel	115:119	arg1	f.a.					162:165	f.a.	162:165	f.a.	162:165	Three strains representing a novel yeast species, Sugiyamaella xiaguanensis f.a., sp.					
30156533	2	21	theme	novel	115:119	arg1	species					127:133	a novel yeast species	113:133	a novel yeast species	113:133	Three strains representing a novel yeast species, Sugiyamaella xiaguanensis f.a., sp.					
30156533	3	22	theme	161041T=CICC	195:206	arg1	nov					172:174	nov	172:174	nov	172:174	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	3	22	theme	161041T=CICC	195:206	arg1	14696T					219:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	5	23	theme	subunit	496:502	arg1	gene					509:512	the large subunit rRNA gene	486:512	the large subunit rRNA gene	486:512	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	24	theme	domains	475:481	arg1	Analysis					453:460	Analysis	453:460	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains	453:569	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	3	25	theme	33167T=CBS	208:217	arg1	nov					172:174	nov	172:174	nov	172:174	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	3	25	theme	33167T=CBS	208:217	arg1	14696T					219:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	5	26	theme	rRNA	504:507	arg1	gene					509:512	the large subunit rRNA gene	486:512	the large subunit rRNA gene	486:512	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	1	27	theme	rotting	72:78	arg1	wood					80:83	rotting wood	72:83	rotting wood	72:83	nov., a yeast species isolated from rotting wood.					
30156533	13	28	theme	separate	1027:1034	arg1	species					1036:1042	a genetically separate species	1013:1042	a genetically separate species	1013:1042	nov. displayed more than 6.5 % nucleotide substitutions from the latter two species, showing that it is a genetically separate species.					
30156533	13	28	theme	separate	1027:1034	arg1	it					1007:1008	it	1007:1008	it	1007:1008	nov. displayed more than 6.5 % nucleotide substitutions from the latter two species, showing that it is a genetically separate species.					
30156533	9	29	theme	1.9 	791:794	arg1	%					795:795	%	795:795	%	795:795	lignohabitans, and by 1.9 % nucleotide substitutions from Su.					
30156533	4	30	theme	novel	323:327	arg1	able					340:343	able	340:343	able	340:343	The novel species is able to assimilate cellobiose, salicin and d-xylose, which was typical of the species of the genus Sugiyamaella.					
30156533	4	30	theme	novel	323:327	arg1	species					329:335	The novel species	319:335	The novel species	319:335	The novel species is able to assimilate cellobiose, salicin and d-xylose, which was typical of the species of the genus Sugiyamaella.					
30156533	1	31	attach	isolated	58:65	arg1	wood					80:83	rotting wood	72:83	rotting wood	72:83	nov., a yeast species isolated from rotting wood.					
30156533	1	31	attach	isolated	58:65	arg2	species					50:56	a yeast species	42:56	a yeast species isolated from rotting wood	42:83	nov., a yeast species isolated from rotting wood.					
30156533	3	32	dep	Henan	281:285	arg1	Provinces					298:306	Provinces	298:306	Provinces	298:306	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	11	33	theme	Su	888:889	arg1	sequences					875:883	The ITS sequences	867:883	The ITS sequences of Su	867:889	The ITS sequences of Su.					
30156533	9	34	theme	nucleotide	797:806	arg1	substitutions					808:820	1.9 % nucleotide substitutions	791:820	1.9 % nucleotide substitutions from Su	791:828	lignohabitans, and by 1.9 % nucleotide substitutions from Su.					
30156533	5	35	theme	D1/D2	469:473	arg1	gene					509:512	the large subunit rRNA gene	486:512	the large subunit rRNA gene	486:512	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	35	theme	D1/D2	469:473	arg1	domains					475:481	the D1/D2 domains	465:481	the D1/D2 domains of the large subunit rRNA gene	465:512	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	35	theme	D1/D2	469:473	arg1	strains					563:569	these strains	557:569	these strains	557:569	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	3	36	attach	isolated	233:240	arg2	nov					172:174	nov	172:174	nov	172:174	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	3	36	attach	isolated	233:240	arg1	samples					260:266	rotting wood samples	247:266	rotting wood samples collected in Henan and Yunnan Provinces, PR China	247:316	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	3	36	attach	isolated	233:240	arg2	14696T					219:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	type strain NYNU 161041T=CICC 33167T=CBS 14696T	178:224	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	4	37	theme	species	418:424	arg1	typical					403:409	typical	403:409	typical	403:409	The novel species is able to assimilate cellobiose, salicin and d-xylose, which was typical of the species of the genus Sugiyamaella.					
30156533	1	38	dep	species	50:56	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., a yeast species isolated from rotting wood.					
30156533	8	39	from	Su	765:766	arg1	substitutions					746:758	1.4 % nucleotide substitutions	729:758	1.4 % nucleotide substitutions from Su	729:766	nov. differed by 1.4 % nucleotide substitutions from Su.					
30156533	0	40	dep	sp	32:33	arg1	f.a.					26:29	f.a.	26:29	f.a.	26:29	Sugiyamaella xiaguanensis f.a., sp.					
30156533	5	41	theme	strains	563:569	arg1	gene					509:512	the large subunit rRNA gene	486:512	the large subunit rRNA gene	486:512	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	41	theme	strains	563:569	arg1	domains					475:481	the D1/D2 domains	465:481	the D1/D2 domains of the large subunit rRNA gene	465:512	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	41	theme	strains	563:569	arg1	regions					546:552	internal transcribed spacer regions	518:552	internal transcribed spacer regions	518:552	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	41	theme	strains	563:569	arg1	strains					563:569	these strains	557:569	these strains	557:569	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	42	theme	gene	509:512	arg1	gene					509:512	the large subunit rRNA gene	486:512	the large subunit rRNA gene	486:512	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	42	theme	gene	509:512	arg1	domains					475:481	the D1/D2 domains	465:481	the D1/D2 domains of the large subunit rRNA gene	465:512	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	42	theme	gene	509:512	arg1	regions					546:552	internal transcribed spacer regions	518:552	internal transcribed spacer regions	518:552	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	5	42	theme	gene	509:512	arg1	strains					563:569	these strains	557:569	these strains	557:569	Analysis of the D1/D2 domains of the large subunit rRNA gene and internal transcribed spacer regions of these strains showed that this species was related to Sugiyamaella lignohabitans and Sugiyamaella marionensis, its closest relatives.					
30156533	3	43	theme	rotting	247:253	arg1	samples					260:266	rotting wood samples	247:266	rotting wood samples collected in Henan and Yunnan Provinces, PR China	247:316	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	13	44	dep	substitutions	951:963	arg1	%					938:938	%	938:938	%	938:938	nov. displayed more than 6.5 % nucleotide substitutions from the latter two species, showing that it is a genetically separate species.					
30156533	3	45	theme	wood	255:258	arg1	samples					260:266	rotting wood samples	247:266	rotting wood samples collected in Henan and Yunnan Provinces, PR China	247:316	nov. (type strain NYNU 161041T=CICC 33167T=CBS 14696T), were isolated from rotting wood samples collected in Henan and Yunnan Provinces, PR China.					
30156533	7	46	theme	xiaguanensis	695:706	arg1	sp					708:709	xiaguanensis sp	695:709	xiaguanensis sp.	695:710	xiaguanensis sp.					
30156533	13	47	dep	%	938:938	arg1	6.5 					934:937	6.5 	934:937	6.5 	934:937	nov. displayed more than 6.5 % nucleotide substitutions from the latter two species, showing that it is a genetically separate species.					
30156533	12	48	theme	xiaguanensis	892:903	arg1	sp					905:906	xiaguanensis sp	892:906	xiaguanensis sp.	892:907	xiaguanensis sp.					
30156533	13	49	theme	latter	974:979	arg1	species					985:991	the latter two species	970:991	the latter two species	970:991	nov. displayed more than 6.5 % nucleotide substitutions from the latter two species, showing that it is a genetically separate species.					
30156533	9	50	from	Su	827:828	arg1	substitutions					808:820	1.9 % nucleotide substitutions	791:820	1.9 % nucleotide substitutions from Su	791:828	lignohabitans, and by 1.9 % nucleotide substitutions from Su.					
30156533	9	50	from	Su	827:828	arg1	lignohabitans					769:781	lignohabitans	769:781	lignohabitans	769:781	lignohabitans, and by 1.9 % nucleotide substitutions from Su.					
29633642	6	0	dep	composition	915:925	arg1	The					911:913	The	911:913	The	911:913	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	5	1	with	YCF	795:797	arg1	ml					815:816	0.95 g/100 ml	804:816	0.95 g/100 ml	804:816	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	3	2	theme	Bifidobacterium	555:569	arg1	M-16V					577:581	Bifidobacterium breve M-16V	555:581	Bifidobacterium breve M-16V	555:581	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	13	3	theme	intestinal	2072:2081	arg1	milieu					2083:2088	a more acidic intestinal milieu	2058:2088	a more acidic intestinal milieu	2058:2088	The synbiotic supplementation is also accompanied with a more acidic intestinal milieu and softer stools.					
29633642	12	4	from	development	1898:1908	arg1	toddlers					1946:1953	healthy toddlers	1938:1953	healthy toddlers	1938:1953	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	11	5	from	week	1675:1678	arg1	difference					1702:1711	the between-group difference	1684:1711	the between-group difference in stool consistency	1684:1732	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	5	from	week	1675:1678	arg1	significant					1752:1762	significant	1752:1762	significant	1752:1762	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	5	from	week	1675:1678	arg1	P<0.001					1777:1783	P<0.001	1777:1783	P<0.001	1777:1783	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	5	from	week	1675:1678	arg1	P=0.004					1765:1771	P=0.004	1765:1771	P=0.004	1765:1771	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	4	6	theme	Thai	664:667	arg1	children					669:676	129 Thai children	660:676	129 Thai children aged 1-3 years	660:691	A cohort of 129 Thai children aged 1-3 years were included in a randomised controlled clinical study.					
29633642	5	7	theme	B.	854:855	arg1	Active-YCF					870:879	Active-YCF	870:879	Active-YCF	870:879	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	5	7	theme	B.	854:855	arg1	M-16V					863:867	B. breve M-16V	854:867	B. breve M-16V (Active-YCF)	854:880	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	12	8	theme	breve	1860:1864	arg1	M-16V					1866:1870	B. breve M-16V	1857:1870	B. breve M-16V	1857:1870	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	4	9	theme	aged	678:681	arg1	children					669:676	129 Thai children	660:676	129 Thai children aged 1-3 years	660:691	A cohort of 129 Thai children aged 1-3 years were included in a randomised controlled clinical study.					
29633642	7	10	dep	increased	1088:1096	arg1	%					1142:1142	mean 27.3%	1133:1142	mean 27.3% at baseline to 33.3%, at week 12	1133:1175	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	1	11	theme	gut	184:186	arg1	microbiota					188:197	toddler gut microbiota	176:197	toddler gut microbiota	176:197	Little is known about the impact of nutrition on toddler gut microbiota.					
29633642	10	12	theme	pudding-like	1601:1612	arg1	consistency					1620:1630	a softer pudding-like stool consistency	1592:1630	a softer pudding-like stool consistency compared to the Control-YCF	1592:1658	The consumption of Active-YCF was associated with a softer pudding-like stool consistency compared to the Control-YCF.					
29633642	6	13	theme	metabolic	931:939	arg1	activity					941:948	metabolic activity	931:948	metabolic activity	931:948	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	2	14	theme	toddler	222:228	arg1	microbiota					234:243	the toddler gut microbiota	218:243	the toddler gut microbiota	218:243	The plasticity of the toddler gut microbiota indicates that nutritional modulation beyond infancy could potentially impact its maturation.					
29633642	5	15	theme	cfu/g	845:849	arg1	YCF					795:797	YCF	795:797	YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF)	795:880	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	5	15	theme	cfu/g	845:849	arg1	Control-YCF					885:895	Control-YCF	885:895	Control-YCF	885:895	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	8	16	theme	intestinal	1356:1365	arg1	milieu					1367:1372	a more acidic intestinal milieu	1342:1372	a more acidic intestinal milieu compared to the Control-YCF	1342:1400	The consumption of Active-YCF was accompanied with a more acidic intestinal milieu compared to the Control-YCF.					
29633642	11	17	theme	between-group	1688:1700	arg1	difference					1702:1711	the between-group difference	1684:1711	the between-group difference in stool consistency	1684:1732	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	17	theme	between-group	1688:1700	arg1	significant					1752:1762	significant	1752:1762	significant	1752:1762	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	17	theme	between-group	1688:1700	arg1	P<0.001					1777:1783	P<0.001	1777:1783	P<0.001	1777:1783	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	17	theme	between-group	1688:1700	arg1	P=0.004					1765:1771	P=0.004	1765:1771	P=0.004	1765:1771	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	7	18	from	week	1169:1172	arg1	baseline					1147:1154	baseline	1147:1154	baseline to 33.3%	1147:1163	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	4	19	theme	controlled	723:732	arg1	study					743:747	a randomised controlled clinical study	710:747	a randomised controlled clinical study	710:747	A cohort of 129 Thai children aged 1-3 years were included in a randomised controlled clinical study.					
29633642	13	20	theme	synbiotic	2007:2015	arg1	supplementation					2017:2031	The synbiotic supplementation	2003:2031	The synbiotic supplementation	2003:2031	The synbiotic supplementation is also accompanied with a more acidic intestinal milieu and softer stools.					
29633642	12	21	theme	Bifidobacterium	1986:2000	arg1	levels					1976:1981	higher levels	1969:1981	higher levels of Bifidobacterium	1969:2000	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	0	22	theme	M-16V	61:65	arg1	mixture					12:18	A synbiotic mixture	0:18	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V	0:65	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.					
29633642	2	23	theme	microbiota	234:243	arg1	plasticity					204:213	The plasticity	200:213	The plasticity of the toddler gut microbiota	200:243	The plasticity of the toddler gut microbiota indicates that nutritional modulation beyond infancy could potentially impact its maturation.					
29633642	0	24	theme	faecal	77:82	arg1	Bifidobacterium					84:98	faecal Bifidobacterium	77:98	faecal Bifidobacterium in healthy young children	77:124	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.					
29633642	1	25	from	impact	153:158	arg1	microbiota					188:197	toddler gut microbiota	176:197	toddler gut microbiota	176:197	Little is known about the impact of nutrition on toddler gut microbiota.					
29633642	12	26	theme	Child	1809:1813	arg1	Formula					1815:1821	A Young Child Formula	1801:1821	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V	1801:1870	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	9	27	theme	Active-YCF	1461:1470	arg1	group					1472:1476	the Active-YCF group	1457:1476	the Active-YCF group	1457:1476	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	6	28	theme	faecal	957:962	arg1	microbiota					964:973	the faecal microbiota	953:973	the faecal microbiota	953:973	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	0	29	theme	young	111:115	arg1	children					117:124	healthy young children	103:124	healthy young children	103:124	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.					
29633642	3	30	from	effect	388:393	arg1	development					590:600	the development	586:600	the development of the faecal microbiota in healthy toddlers	586:645	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	11	31	dep	significant	1752:1762	arg1	difference					1702:1711	the between-group difference	1684:1711	the between-group difference in stool consistency	1684:1732	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	31	dep	significant	1752:1762	arg1	P=0.004					1765:1771	P=0.004	1765:1771	P=0.004	1765:1771	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	31	dep	significant	1752:1762	arg1	significant					1752:1762	significant	1752:1762	significant	1752:1762	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	31	dep	significant	1752:1762	arg1	P<0.001					1777:1783	P<0.001	1777:1783	P<0.001	1777:1783	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	6	32	theme	stool	1043:1047	arg1	samples					1049:1055	the stool samples	1039:1055	the stool samples	1039:1055	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	7	33	from	baseline	1147:1154	arg1	%					1142:1142	mean 27.3%	1133:1142	mean 27.3% at baseline to 33.3%, at week 12	1133:1175	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	5	34	with	Control-YCF	885:895	arg1	ml					815:816	0.95 g/100 ml	804:816	0.95 g/100 ml	804:816	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	3	35	theme	chain	458:462	arg1	galactooligosaccharides					464:486	short chain galactooligosaccharides	452:486	short chain galactooligosaccharides	452:486	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	9	36	theme	7.05	1495:1498	arg1	median					1485:1490	a median	1483:1490	a median of 7.05 at baseline to 6.79 at week 12 (P<0.001)	1483:1539	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	3	37	theme	Child	414:418	arg1	YCF					429:431	YCF	429:431	YCF	429:431	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	3	37	theme	Child	414:418	arg1	Formula					420:426	Young Child Formula	408:426	Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1)	408:549	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	7	38	theme	mean	1133:1136	arg1	%					1142:1142	mean 27.3%	1133:1142	mean 27.3% at baseline to 33.3%, at week 12	1133:1175	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	0	39	theme	scGOS/lcFOS	23:33	arg1	mixture					12:18	A synbiotic mixture	0:18	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V	0:65	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.					
29633642	7	40	from	baseline	1220:1227	arg1	change					1208:1213	change	1208:1213	change from baseline at week 12	1208:1238	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	7	40	from	baseline	1220:1227	arg1	difference					1194:1203	a difference	1192:1203	a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030)	1192:1288	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	6	41	theme	immunoglobulin	1003:1016	arg1	A					1018:1018	secretory immunoglobulin A	993:1018	secretory immunoglobulin A	993:1018	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	8	42	theme	Active-YCF	1310:1319	arg1	consumption					1295:1305	The consumption	1291:1305	The consumption of Active-YCF	1291:1319	The consumption of Active-YCF was accompanied with a more acidic intestinal milieu compared to the Control-YCF.					
29633642	10	43	theme	softer	1594:1599	arg1	consistency					1620:1630	a softer pudding-like stool consistency	1592:1630	a softer pudding-like stool consistency compared to the Control-YCF	1592:1658	The consumption of Active-YCF was associated with a softer pudding-like stool consistency compared to the Control-YCF.					
29633642	0	44	theme	Bifidobacterium	39:53	arg1	M-16V					61:65	Bifidobacterium breve M-16V	39:65	Bifidobacterium breve M-16V	39:65	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.					
29633642	7	45	from	week	1232:1235	arg1	baseline					1220:1227	baseline	1220:1227	baseline at week 12	1220:1238	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	2	46	theme	nutritional	260:270	arg1	modulation					272:281	nutritional modulation	260:281	nutritional modulation beyond infancy	260:296	The plasticity of the toddler gut microbiota indicates that nutritional modulation beyond infancy could potentially impact its maturation.					
29633642	12	47	theme	healthy	1938:1944	arg1	toddlers					1946:1953	healthy toddlers	1938:1953	healthy toddlers	1938:1953	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	13	48	theme	softer	2094:2099	arg1	stools					2101:2106	softer stools	2094:2106	softer stools	2094:2106	The synbiotic supplementation is also accompanied with a more acidic intestinal milieu and softer stools.					
29633642	10	49	theme	stool	1614:1618	arg1	consistency					1620:1630	a softer pudding-like stool consistency	1592:1630	a softer pudding-like stool consistency compared to the Control-YCF	1592:1658	The consumption of Active-YCF was associated with a softer pudding-like stool consistency compared to the Control-YCF.					
29633642	12	50	theme	microbiota	1924:1933	arg1	development					1898:1908	the development	1894:1908	the development of the faecal microbiota in healthy toddlers	1894:1953	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	11	51	from	difference	1702:1711	arg1	consistency					1722:1732	stool consistency	1716:1732	stool consistency	1716:1732	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	7	52	theme	Active-YCF	1077:1086	arg1	consumption					1062:1072	The consumption	1058:1072	The consumption of Active-YCF	1058:1086	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	13	53	theme	acidic	2065:2070	arg1	milieu					2083:2088	a more acidic intestinal milieu	2058:2088	a more acidic intestinal milieu	2058:2088	The synbiotic supplementation is also accompanied with a more acidic intestinal milieu and softer stools.					
29633642	3	54	theme	chain	497:501	arg1	fructooligosaccharides					503:524	long chain fructooligosaccharides	492:524	long chain fructooligosaccharides	492:524	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	5	55	theme	breve	857:861	arg1	Active-YCF					870:879	Active-YCF	870:879	Active-YCF	870:879	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	5	55	theme	breve	857:861	arg1	M-16V					863:867	B. breve M-16V	854:867	B. breve M-16V (Active-YCF)	854:880	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	1	56	theme	nutrition	163:171	arg1	impact					153:158	the impact	149:158	the impact of nutrition on toddler gut microbiota	149:197	Little is known about the impact of nutrition on toddler gut microbiota.					
29633642	7	57	theme	Bifidobacterium	1116:1130	arg1	proportion					1102:1111	the proportion	1098:1111	the proportion of Bifidobacterium	1098:1130	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	7	58	from	difference	1194:1203	arg1	baseline					1220:1227	baseline	1220:1227	baseline at week 12	1220:1238	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	7	58	from	difference	1194:1203	arg1	change					1208:1213	change	1208:1213	change from baseline at week 12	1208:1238	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	1	59	theme	toddler	176:182	arg1	microbiota					188:197	toddler gut microbiota	176:197	toddler gut microbiota	176:197	Little is known about the impact of nutrition on toddler gut microbiota.					
29633642	12	60	theme	B.	1857:1858	arg1	M-16V					1866:1870	B. breve M-16V	1857:1870	B. breve M-16V	1857:1870	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	4	61	theme	children	669:676	arg1	cohort					650:655	A cohort	648:655	A cohort of 129 Thai children aged 1-3 years	648:691	A cohort of 129 Thai children aged 1-3 years were included in a randomised controlled clinical study.					
29633642	3	62	theme	breve	571:575	arg1	M-16V					577:581	Bifidobacterium breve M-16V	555:581	Bifidobacterium breve M-16V	555:581	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	5	63	theme	scGOS/lcFOS	821:831	arg1	YCF					795:797	YCF	795:797	YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF)	795:880	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	5	63	theme	scGOS/lcFOS	821:831	arg1	Control-YCF					885:895	Control-YCF	885:895	Control-YCF	885:895	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	11	64	from	week	1664:1667	arg1	difference					1702:1711	the between-group difference	1684:1711	the between-group difference in stool consistency	1684:1732	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	64	from	week	1664:1667	arg1	significant					1752:1762	significant	1752:1762	significant	1752:1762	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	64	from	week	1664:1667	arg1	P<0.001					1777:1783	P<0.001	1777:1783	P<0.001	1777:1783	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	11	64	from	week	1664:1667	arg1	P=0.004					1765:1771	P=0.004	1765:1771	P=0.004	1765:1771	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	8	65	theme	acidic	1349:1354	arg1	milieu					1367:1372	a more acidic intestinal milieu	1342:1372	a more acidic intestinal milieu compared to the Control-YCF	1342:1400	The consumption of Active-YCF was accompanied with a more acidic intestinal milieu compared to the Control-YCF.					
29633642	2	66	theme	gut	230:232	arg1	microbiota					234:243	the toddler gut microbiota	218:243	the toddler gut microbiota	218:243	The plasticity of the toddler gut microbiota indicates that nutritional modulation beyond infancy could potentially impact its maturation.					
29633642	5	67	theme	M-16V	863:867	arg1	scGOS/lcFOS					821:831	scGOS/lcFOS	821:831	scGOS/lcFOS	821:831	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	5	67	theme	M-16V	863:867	arg1	cfu/g					845:849	1.8×107 cfu/g	837:849	1.8×107 cfu/g	837:849	The children were assigned to receive either YCF with 0.95 g/100 ml of scGOS/lcFOS and 1.8×107 cfu/g of B. breve M-16V (Active-YCF) or Control-YCF for 12 weeks.					
29633642	11	68	theme	stool	1716:1720	arg1	consistency					1722:1732	stool consistency	1716:1732	stool consistency	1716:1732	At week 6 and week 12, the between-group difference in stool consistency was statistically significant (P=0.004 and P<0.001, respectively).					
29633642	12	69	theme	higher	1969:1974	arg1	levels					1976:1981	higher levels	1969:1981	higher levels of Bifidobacterium	1969:2000	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	3	70	theme	healthy	630:636	arg1	toddlers					638:645	healthy toddlers	630:645	healthy toddlers	630:645	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	9	71	theme	pH	1407:1408	arg1	value					1410:1414	The pH value	1403:1414	The pH value	1403:1414	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	6	72	theme	secretory	993:1001	arg1	A					1018:1018	secretory immunoglobulin A	993:1018	secretory immunoglobulin A	993:1018	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	7	73	theme	%	1278:1278	arg1	Control					1263:1269	Control	1263:1269	Control	1263:1269	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	4	74	theme	randomised	712:721	arg1	study					743:747	a randomised controlled clinical study	710:747	a randomised controlled clinical study	710:747	A cohort of 129 Thai children aged 1-3 years were included in a randomised controlled clinical study.					
29633642	3	75	theme	microbiota	616:625	arg1	development					590:600	the development	586:600	the development of the faecal microbiota in healthy toddlers	586:645	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	9	76	from	median	1485:1490	arg1	baseline					1503:1510	baseline	1503:1510	baseline	1503:1510	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	9	77	dep	7.05	1495:1498	arg1	to					1512:1513	to	1512:1513	to	1512:1513	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	9	77	dep	7.05	1495:1498	arg1	P<0.001					1532:1538	P<0.001	1532:1538	P<0.001	1532:1538	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	9	77	dep	7.05	1495:1498	arg1	6.79					1515:1518	6.79	1515:1518	6.79	1515:1518	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	12	78	theme	Young	1803:1807	arg1	Formula					1815:1821	A Young Child Formula	1801:1821	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V	1801:1870	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	7	79	dep	%	1142:1142	arg1	P=0.012					1178:1184	P=0.012	1178:1184	P=0.012	1178:1184	The consumption of Active-YCF increased the proportion of Bifidobacterium (mean 27.3% at baseline to 33.3%, at week 12, P=0.012) with a difference in change from baseline at week 12 between the Active and Control of 7.48% (P=0.030).					
29633642	0	80	from	Bifidobacterium	84:98	arg1	children					117:124	healthy young children	103:124	healthy young children	103:124	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.					
29633642	0	81	theme	healthy	103:109	arg1	children					117:124	healthy young children	103:124	healthy young children	103:124	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.					
29633642	6	82	theme	microbiota	964:973	arg1	composition					915:925	composition	915:925	composition	915:925	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	6	82	theme	microbiota	964:973	arg1	level					984:988	the level	980:988	the level of secretory immunoglobulin A	980:1018	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	6	82	theme	microbiota	964:973	arg1	activity					941:948	metabolic activity	931:948	metabolic activity	931:948	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	3	83	theme	study	361:365	arg1	objective					343:351	The objective	339:351	The objective of this study	339:365	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	0	84	theme	synbiotic	2:10	arg1	mixture					12:18	A synbiotic mixture	0:18	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V	0:65	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.					
29633642	9	85	from	week	1523:1526	arg1	P<0.001					1532:1538	P<0.001	1532:1538	P<0.001	1532:1538	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	9	85	from	week	1523:1526	arg1	6.79					1515:1518	6.79	1515:1518	6.79	1515:1518	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	3	86	dep	Formula	420:426	arg1	scGOS/lcFOS					527:537	scGOS/lcFOS	527:537	scGOS/lcFOS	527:537	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	3	86	dep	Formula	420:426	arg1	9:1					546:548	ratio 9:1	540:548	ratio 9:1	540:548	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	4	87	theme	clinical	734:741	arg1	study					743:747	a randomised controlled clinical study	710:747	a randomised controlled clinical study	710:747	A cohort of 129 Thai children aged 1-3 years were included in a randomised controlled clinical study.					
29633642	3	88	from	development	590:600	arg1	toddlers					638:645	healthy toddlers	630:645	healthy toddlers	630:645	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	10	89	theme	Active-YCF	1561:1570	arg1	consumption					1546:1556	The consumption	1542:1556	The consumption of Active-YCF	1542:1570	The consumption of Active-YCF was associated with a softer pudding-like stool consistency compared to the Control-YCF.					
29633642	9	90	from	baseline	1503:1510	arg1	median					1485:1490	a median	1483:1490	a median of 7.05 at baseline to 6.79 at week 12 (P<0.001)	1483:1539	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	9	90	from	baseline	1503:1510	arg1	7.05					1495:1498	7.05	1495:1498	7.05	1495:1498	The pH value decreased statistically significantly in the Active-YCF group from a median of 7.05 at baseline to 6.79 at week 12 (P<0.001).					
29633642	6	91	theme	A	1018:1018	arg1	composition					915:925	composition	915:925	composition	915:925	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	6	91	theme	A	1018:1018	arg1	level					984:988	the level	980:988	the level of secretory immunoglobulin A	980:1018	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	6	91	theme	A	1018:1018	arg1	activity					941:948	metabolic activity	931:948	metabolic activity	931:948	The composition and metabolic activity of the faecal microbiota, and the level of secretory immunoglobulin A were determined in the stool samples.					
29633642	3	92	theme	short	452:456	arg1	galactooligosaccharides					464:486	short chain galactooligosaccharides	452:486	short chain galactooligosaccharides	452:486	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	0	93	theme	breve	55:59	arg1	M-16V					61:65	Bifidobacterium breve M-16V	39:65	Bifidobacterium breve M-16V	39:65	A synbiotic mixture of scGOS/lcFOS and Bifidobacterium breve M-16V increases faecal Bifidobacterium in healthy young children.					
29633642	3	94	theme	Young	408:412	arg1	YCF					429:431	YCF	429:431	YCF	429:431	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	3	94	theme	Young	408:412	arg1	Formula					420:426	Young Child Formula	408:426	Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1)	408:549	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	3	95	theme	faecal	609:614	arg1	microbiota					616:625	the faecal microbiota	605:625	the faecal microbiota	605:625	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	3	96	theme	long	492:495	arg1	fructooligosaccharides					503:524	long chain fructooligosaccharides	492:524	long chain fructooligosaccharides	492:524	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	12	97	theme	faecal	1917:1922	arg1	microbiota					1924:1933	the faecal microbiota	1913:1933	the faecal microbiota	1913:1933	A Young Child Formula supplemented with scGOS/lcFOS and B. breve M-16V positively influences the development of the faecal microbiota in healthy toddlers by supporting higher levels of Bifidobacterium.					
29633642	3	98	theme	ratio	540:544	arg1	scGOS/lcFOS					527:537	scGOS/lcFOS	527:537	scGOS/lcFOS	527:537	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
29633642	3	98	theme	ratio	540:544	arg1	9:1					546:548	ratio 9:1	540:548	ratio 9:1	540:548	The objective of this study was to determine the effect of consuming Young Child Formula (YCF) supplemented with short chain galactooligosaccharides and long chain fructooligosaccharides (scGOS/lcFOS, ratio 9:1) and Bifidobacterium breve M-16V on the development of the faecal microbiota in healthy toddlers.					
30276716	6	0	theme	identified	1154:1163	arg1	proteins					1165:1172	identified proteins	1154:1172	identified proteins	1154:1172	Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.					
30276716	5	1	with	comparison	1003:1012	arg1	12					1019:1020	12	1019:1020	12	1019:1020	Predominantly, protease inhibitors II were identified for the protein samples obtained by 100 °C and here we identified 7 spots in comparison with 12 identified for the 80 °C samples.					
30276716	10	2	theme	same	1747:1750	arg1	time					1752:1755	the same time	1743:1755	the same time	1743:1755	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	6	3	with	Samples	1056:1062	arg1	activities					1100:1109	low antifungal activities	1085:1109	low antifungal activities	1085:1109	Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.					
30276716	6	4	theme	detected	1141:1148	arg1	variability					1126:1136	high variability	1121:1136	high variability of detected and identified proteins	1121:1172	Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.					
30276716	3	5	theme	species	677:683	arg1	strains					653:659	five strains	648:659	five strains of two Fusarium species	648:683	The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml-1.					
30276716	4	6	theme	tuber	836:840	arg1	inhibitors					851:860	potato tuber protease inhibitors I and II	829:869	potato tuber protease inhibitors I and II	829:869	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	6	theme	tuber	836:840	arg1	II					868:869	II	868:869	II	868:869	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	6	theme	tuber	836:840	arg1	I					862:862	I	862:862	I	862:862	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	9	7	from	temperatures	1506:1517	arg1	stability					1488:1496	Functional stability	1477:1496	Functional stability at high temperatures	1477:1517	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	7	from	temperatures	1506:1517	arg1	activity					1534:1541	antifungal activity	1523:1541	antifungal activity of isolated potato protease inhibitors I and II	1523:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	3	8	theme	protein	543:549	arg1	samples					551:557	The protein samples	539:557	The protein samples of 80 °C	539:566	The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml-1.					
30276716	1	9	theme	manufacture	200:210	arg1	effluent					179:186	effluent	179:186	effluent of a starch manufacture	179:210	Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 °C).					
30276716	0	10	theme	temperature	130:140	arg1	regimes					142:148	different temperature regimes	120:148	different temperature regimes	120:148	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	9	11	theme	new	1702:1704	arg1	products					1706:1713	new products	1702:1713	new products for starch manufactures	1702:1737	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	8	12	theme	thermal	1420:1426	arg1	unstableness					1428:1439	thermal unstableness	1420:1439	thermal unstableness of Kunitz-type protease inhibitors	1420:1474	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	10	13	theme	fraction	1792:1799	arg1	utilization					1758:1768	utilization	1758:1768	utilization of the stable protein fraction of waste deproteinized potato water	1758:1835	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	9	14	theme	Functional	1477:1486	arg1	stability					1488:1496	Functional stability	1477:1496	Functional stability at high temperatures	1477:1517	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	10	15	theme	stable	1777:1782	arg1	fraction					1792:1799	the stable protein fraction	1773:1799	the stable protein fraction of waste deproteinized potato water	1773:1835	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	8	16	theme	protease	1456:1463	arg1	inhibitors					1465:1474	Kunitz-type protease inhibitors	1444:1474	Kunitz-type protease inhibitors	1444:1474	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	10	17	theme	potato	1824:1829	arg1	water					1831:1835	waste deproteinized potato water	1804:1835	waste deproteinized potato water	1804:1835	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	0	18	theme	proteins	67:74	arg1	characterization					10:25	Proteomic characterization	0:25	Proteomic characterization	0:25	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	0	18	theme	proteins	67:74	arg1	activity					42:49	antifungal activity	31:49	antifungal activity	31:49	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	3	19	theme	highest	582:588	arg1	activity					601:608	the highest antifungal activity	578:608	the highest antifungal activity	578:608	The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml-1.					
30276716	9	20	theme	antifungal	1523:1532	arg1	activity					1534:1541	antifungal activity	1523:1541	antifungal activity of isolated potato protease inhibitors I and II	1523:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	3	21	theme	average	618:624	arg1	value					626:630	its average value	614:630	its average value of IC50 against five strains of two Fusarium species	614:683	The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml-1.					
30276716	7	22	theme	various	1189:1195	arg1	representatives					1197:1211	various representatives	1189:1211	various representatives of aspartic, cysteine, and serine protease inhibitors	1189:1265	We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples.					
30276716	0	23	theme	production	97:106	arg1	waste					108:112	starch production waste	90:112	starch production waste under different temperature regimes	90:148	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	9	24	theme	potato	1555:1560	arg1	inhibitors					1571:1580	isolated potato protease inhibitors I and II	1546:1589	isolated potato protease inhibitors I and II	1546:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	24	theme	potato	1555:1560	arg1	II					1588:1589	II	1588:1589	II	1588:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	24	theme	potato	1555:1560	arg1	I					1582:1582	I	1582:1582	I	1582:1582	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	8	25	contain	contained	1312:1320	arg2	inhibitors					1343:1352	Kunitz-type protease inhibitors	1322:1352	Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors	1322:1474	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	8	25	contain	contained	1312:1320	arg1	samples					1299:1305	These samples	1293:1305	These samples	1293:1305	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	2	26	with	comparison	493:502	arg1	variants					513:520	the variants	509:520	the variants of 40 and 60 °C	509:536	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	9	27	theme	inhibitors	1571:1580	arg1	stability					1488:1496	Functional stability	1477:1496	Functional stability at high temperatures	1477:1517	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	27	theme	inhibitors	1571:1580	arg1	activity					1534:1541	antifungal activity	1523:1541	antifungal activity of isolated potato protease inhibitors I and II	1523:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	28	theme	agricultural	1670:1681	arg1	industry					1683:1690	agricultural industry	1670:1690	agricultural industry	1670:1690	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	0	29	theme	Proteomic	0:8	arg1	characterization					10:25	Proteomic characterization	0:25	Proteomic characterization	0:25	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	2	30	theme	structural	410:419	arg1	stability					421:429	structural stability	410:429	structural stability	410:429	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	5	31	theme	protein	934:940	arg1	samples					942:948	the protein samples	930:948	the protein samples obtained by 100 °C	930:967	Predominantly, protease inhibitors II were identified for the protein samples obtained by 100 °C and here we identified 7 spots in comparison with 12 identified for the 80 °C samples.					
30276716	7	32	theme	samples	1284:1290	arg1	types					1275:1279	both types	1270:1279	both types of samples	1270:1290	We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples.					
30276716	2	33	theme	trypsin	445:451	arg1	activities					479:488	trypsin inhibitory and antifungal activities	445:488	trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C	445:536	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	7	34	theme	aspartic	1216:1223	arg1	cysteine					1226:1233	cysteine	1226:1233	cysteine	1226:1233	We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples.					
30276716	0	35	theme	potato	54:59	arg1	proteins					67:74	potato tuber proteins	54:74	potato tuber proteins	54:74	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	10	36	theme	water	1831:1835	arg1	fraction					1792:1799	the stable protein fraction	1773:1799	the stable protein fraction of waste deproteinized potato water	1773:1835	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	6	37	theme	low	1085:1087	arg1	activities					1100:1109	low antifungal activities	1085:1109	low antifungal activities	1085:1109	Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.					
30276716	10	38	theme	waste	1804:1808	arg1	water					1831:1835	waste deproteinized potato water	1804:1835	waste deproteinized potato water	1804:1835	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	10	39	theme	resources	1887:1895	arg1	exploitation					1846:1857	exploitation	1846:1857	exploitation of potato starch production resources	1846:1895	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	9	40	theme	usage	1630:1634	arg1	potential					1603:1611	the potential	1599:1611	the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry	1599:1690	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	7	41	theme	protease	1247:1254	arg1	inhibitors					1256:1265	serine protease inhibitors	1240:1265	serine protease inhibitors	1240:1265	We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples.					
30276716	8	42	theme	Kunitz-type	1444:1454	arg1	inhibitors					1465:1474	Kunitz-type protease inhibitors	1444:1474	Kunitz-type protease inhibitors	1444:1474	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	10	43	theme	starch	1869:1874	arg1	resources					1887:1895	potato starch production resources	1862:1895	potato starch production resources	1862:1895	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	4	44	theme	low-molecular	781:793	arg1	7-17 kDa					805:812	7-17 kDa	805:812	7-17 kDa	805:812	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	44	theme	low-molecular	781:793	arg1	proteins					795:802	low-molecular proteins	781:802	low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II	781:869	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	45	dep	inhibitors	851:860	arg1	inhibitors					851:860	potato tuber protease inhibitors I and II	829:869	potato tuber protease inhibitors I and II	829:869	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	45	dep	inhibitors	851:860	arg1	II					868:869	II	868:869	II	868:869	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	45	dep	inhibitors	851:860	arg1	I					862:862	I	862:862	I	862:862	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	6	46	theme	40	1067:1068	arg1	Samples					1056:1062	Samples	1056:1062	Samples of 40 and 60 °C with low antifungal activities	1056:1109	Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.					
30276716	3	47	theme	Fusarium	668:675	arg1	species					677:683	two Fusarium species	664:683	two Fusarium species	664:683	The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml-1.					
30276716	2	48	theme	potato	335:340	arg1	juice					342:346	potato juice	335:346	potato juice at 80 and 100 °C	335:363	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	4	49	theme	protease	842:849	arg1	inhibitors					851:860	potato tuber protease inhibitors I and II	829:869	potato tuber protease inhibitors I and II	829:869	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	49	theme	protease	842:849	arg1	II					868:869	II	868:869	II	868:869	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	49	theme	protease	842:849	arg1	I					862:862	I	862:862	I	862:862	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	7	50	theme	cysteine	1226:1233	arg1	representatives					1197:1211	various representatives	1189:1211	various representatives of aspartic, cysteine, and serine protease inhibitors	1189:1265	We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples.					
30276716	2	51	from	treatment	322:330	arg1	100 °C					358:363	100 °C	358:363	100 °C	358:363	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	2	51	from	treatment	322:330	arg1	80					351:352	80	351:352	80	351:352	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	7	52	theme	inhibitors	1256:1265	arg1	representatives					1197:1211	various representatives	1189:1211	various representatives of aspartic, cysteine, and serine protease inhibitors	1189:1265	We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples.					
30276716	8	53	located	found	1368:1372	arg1	samples					1395:1401	the 80 and 100 °C samples	1377:1401	the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors	1377:1474	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	8	53	located	found	1368:1372	arg2	inhibitors					1343:1352	Kunitz-type protease inhibitors	1322:1352	Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors	1322:1474	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	1	54	theme	starch	193:198	arg1	manufacture					200:210	a starch manufacture	191:210	a starch manufacture	191:210	Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 °C).					
30276716	4	55	theme	potato	829:834	arg1	inhibitors					851:860	potato tuber protease inhibitors I and II	829:869	potato tuber protease inhibitors I and II	829:869	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	55	theme	potato	829:834	arg1	II					868:869	II	868:869	II	868:869	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	4	55	theme	potato	829:834	arg1	I					862:862	I	862:862	I	862:862	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	6	56	theme	high	1121:1124	arg1	variability					1126:1136	high variability	1121:1136	high variability of detected and identified proteins	1121:1172	Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.					
30276716	2	57	theme	40	525:526	arg1	variants					513:520	the variants	509:520	the variants of 40 and 60 °C	509:536	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	5	58	theme	80 °C	1041:1045	arg1	samples					1047:1053	the 80 °C samples	1037:1053	the 80 °C samples	1037:1053	Predominantly, protease inhibitors II were identified for the protein samples obtained by 100 °C and here we identified 7 spots in comparison with 12 identified for the 80 °C samples.					
30276716	3	59	theme	80 °C	562:566	arg1	samples					551:557	The protein samples	539:557	The protein samples of 80 °C	539:566	The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml-1.					
30276716	1	60	theme	different	221:229	arg1	temperatures					241:252	different isolation temperatures	221:252	different isolation temperatures (40, 60, 80, and 100 °C)	221:277	Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 °C).					
30276716	1	61	dep	temperatures	241:252	arg1	80					263:264	80	263:264	80	263:264	Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 °C).					
30276716	1	61	dep	temperatures	241:252	arg1	100 °C					271:276	100 °C	271:276	100 °C	271:276	Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 °C).					
30276716	10	62	theme	protein	1784:1790	arg1	fraction					1792:1799	the stable protein fraction	1773:1799	the stable protein fraction of waste deproteinized potato water	1773:1835	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	5	63	theme	protease	887:894	arg1	inhibitors					896:905	protease inhibitors II	887:908	protease inhibitors II	887:908	Predominantly, protease inhibitors II were identified for the protein samples obtained by 100 °C and here we identified 7 spots in comparison with 12 identified for the 80 °C samples.					
30276716	9	64	theme	starch	1719:1724	arg1	manufactures					1726:1737	starch manufactures	1719:1737	starch manufactures	1719:1737	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	8	65	theme	inhibitors	1465:1474	arg1	unstableness					1428:1439	thermal unstableness	1420:1439	thermal unstableness of Kunitz-type protease inhibitors	1420:1474	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	9	66	dep	inhibitors	1571:1580	arg1	inhibitors					1571:1580	isolated potato protease inhibitors I and II	1546:1589	isolated potato protease inhibitors I and II	1546:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	66	dep	inhibitors	1571:1580	arg1	II					1588:1589	II	1588:1589	II	1588:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	66	dep	inhibitors	1571:1580	arg1	I					1582:1582	I	1582:1582	I	1582:1582	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	3	67	theme	antifungal	590:599	arg1	activity					601:608	the highest antifungal activity	578:608	the highest antifungal activity	578:608	The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml-1.					
30276716	9	68	theme	high	1501:1504	arg1	temperatures					1506:1517	high temperatures	1501:1517	high temperatures	1501:1517	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	2	69	theme	60 °C	532:536	arg1	variants					513:520	the variants	509:520	the variants of 40 and 60 °C	509:536	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	8	70	theme	Kunitz-type	1322:1332	arg1	inhibitors					1343:1352	Kunitz-type protease inhibitors	1322:1352	Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors	1322:1474	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	10	71	theme	deproteinized	1810:1822	arg1	water					1831:1835	waste deproteinized potato water	1804:1835	waste deproteinized potato water	1804:1835	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	2	72	theme	antifungal	468:477	arg1	activities					479:488	trypsin inhibitory and antifungal activities	445:488	trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C	445:536	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	6	73	theme	proteins	1165:1172	arg1	variability					1126:1136	high variability	1121:1136	high variability of detected and identified proteins	1121:1172	Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.					
30276716	0	74	theme	starch	90:95	arg1	waste					108:112	starch production waste	90:112	starch production waste under different temperature regimes	90:148	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	2	75	theme	inhibitory	453:462	arg1	activities					479:488	trypsin inhibitory and antifungal activities	445:488	trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C	445:536	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	9	76	theme	isolated	1546:1553	arg1	inhibitors					1571:1580	isolated potato protease inhibitors I and II	1546:1589	isolated potato protease inhibitors I and II	1546:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	76	theme	isolated	1546:1553	arg1	II					1588:1589	II	1588:1589	II	1588:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	76	theme	isolated	1546:1553	arg1	I					1582:1582	I	1582:1582	I	1582:1582	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	6	77	theme	60 °C	1074:1078	arg1	Samples					1056:1062	Samples	1056:1062	Samples of 40 and 60 °C with low antifungal activities	1056:1109	Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.					
30276716	0	78	theme	different	120:128	arg1	regimes					142:148	different temperature regimes	120:148	different temperature regimes	120:148	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	9	79	theme	protease	1562:1569	arg1	inhibitors					1571:1580	isolated potato protease inhibitors I and II	1546:1589	isolated potato protease inhibitors I and II	1546:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	79	theme	protease	1562:1569	arg1	II					1588:1589	II	1588:1589	II	1588:1589	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	79	theme	protease	1562:1569	arg1	I					1582:1582	I	1582:1582	I	1582:1582	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	8	80	theme	protease	1334:1341	arg1	inhibitors					1343:1352	Kunitz-type protease inhibitors	1322:1352	Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors	1322:1474	These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors.					
30276716	3	81	theme	IC50	635:638	arg1	value					626:630	its average value	614:630	its average value of IC50 against five strains of two Fusarium species	614:683	The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml-1.					
30276716	0	82	theme	antifungal	31:40	arg1	activity					42:49	antifungal activity	31:49	antifungal activity	31:49	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	10	83	theme	potato	1862:1867	arg1	resources					1887:1895	potato starch production resources	1862:1895	potato starch production resources	1862:1895	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	9	84	from	potential	1603:1611	arg1	food					1639:1642	food	1639:1642	food	1639:1642	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	84	from	potential	1603:1611	arg1	feed					1645:1648	feed	1645:1648	feed	1645:1648	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	84	from	potential	1603:1611	arg1	pharmaceutical					1651:1664	pharmaceutical	1651:1664	pharmaceutical	1651:1664	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	9	84	from	potential	1603:1611	arg1	industry					1683:1690	agricultural industry	1670:1690	agricultural industry	1670:1690	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	0	85	theme	tuber	61:65	arg1	proteins					67:74	potato tuber proteins	54:74	potato tuber proteins	54:74	Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes.					
30276716	2	86	from	80	351:352	arg1	treatment					322:330	treatment	322:330	treatment of potato juice at 80 and 100 °C	322:363	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	2	86	from	80	351:352	arg1	juice					342:346	potato juice	335:346	potato juice at 80 and 100 °C	335:363	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	6	87	theme	antifungal	1089:1098	arg1	activities					1100:1109	low antifungal activities	1085:1109	low antifungal activities	1085:1109	Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.					
30276716	2	88	from	activities	479:488	arg1	comparison					493:502	comparison	493:502	comparison with the variants of 40 and 60 °C	493:536	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	9	89	theme	fraction	1621:1628	arg1	usage					1630:1634	this fraction usage	1616:1634	this fraction usage	1616:1634	Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures.					
30276716	2	90	from	100 °C	358:363	arg1	treatment					322:330	treatment	322:330	treatment of potato juice at 80 and 100 °C	322:363	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	2	90	from	100 °C	358:363	arg1	juice					342:346	potato juice	335:346	potato juice at 80 and 100 °C	335:363	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
30276716	7	91	theme	serine	1240:1245	arg1	inhibitors					1256:1265	serine protease inhibitors	1240:1265	serine protease inhibitors	1240:1265	We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples.					
30276716	4	92	theme	protein	738:744	arg1	samples					746:752	The 80 °C protein samples	728:752	The 80 °C protein samples	728:752	The 80 °C protein samples consisted predominantly of low-molecular proteins (7-17 kDa) identified as potato tuber protease inhibitors I and II.					
30276716	10	93	theme	production	1876:1885	arg1	resources					1887:1895	potato starch production resources	1862:1895	potato starch production resources	1862:1895	At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.					
30276716	1	94	theme	isolation	231:239	arg1	temperatures					241:252	different isolation temperatures	221:252	different isolation temperatures (40, 60, 80, and 100 °C)	221:277	Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 °C).					
30276716	2	95	theme	juice	342:346	arg1	treatment					322:330	treatment	322:330	treatment of potato juice at 80 and 100 °C	322:363	The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C.					
29398251	6	0	from	cell-wall-metabolism	1085:1104	arg1	cells					1117:1121	growing cells	1109:1121	growing cells	1109:1121	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	6	1	theme	peptidoglycan	1044:1056	arg1	synthesis					1058:1066	peptidoglycan synthesis	1044:1066	peptidoglycan synthesis	1044:1066	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	5	2	theme	pneumococcal	802:813	arg1	analysis					840:847	the pneumococcal intracellular metabolome analysis	798:847	the pneumococcal intracellular metabolome analysis	798:847	Employing our validated protocol for the pneumococcal intracellular metabolome analysis, a time resolved snapshot of the primary metabolism of pneumococci was obtained.					
29398251	3	3	theme	chemical	524:531	arg1	medium					541:546	a chemical defined medium	522:546	a chemical defined medium composed of typical host metabolites	522:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	7	4	theme	external	1202:1209	arg1	nutrients					1224:1232	external host derived nutrients	1202:1232	external host derived nutrients such as nucleosides	1202:1252	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	7	4	theme	external	1202:1209	arg1	nucleosides					1242:1252	nucleosides	1242:1252	nucleosides	1242:1252	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	4	5	theme	1H-NMR	612:617	arg1	combination					597:607	a combination	595:607	a combination of 1H-NMR, HPLC-MS and GC-MS methods	595:644	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	3	6	theme	typical	560:566	arg1	metabolites					573:583	typical host metabolites	560:583	typical host metabolites	560:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	1	7	theme	severe	171:176	arg1	pneumonia					197:205	pneumonia	197:205	pneumonia	197:205	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	1	7	theme	severe	171:176	arg1	diseases					178:185	severe diseases	171:185	severe diseases including pneumonia and septicemia	171:220	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	1	7	theme	severe	171:176	arg1	septicemia					211:220	septicemia	211:220	septicemia	211:220	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	6	8	theme	metabolic	948:956	arg1	profile					958:964	The intracellular metabolic profile	930:964	The intracellular metabolic profile	930:964	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	3	9	theme	host	568:571	arg1	metabolites					573:583	typical host metabolites	560:583	typical host metabolites	560:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	4	10	theme	metabolic	738:746	arg1	composition					748:758	the intracellular metabolic composition	720:758	metabolites as well as the intracellular metabolic composition	697:758	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	8	11	theme	essential	1261:1269	arg1	targets					1297:1303	new targets	1293:1303	new targets in the drug development against S. pneumoniae	1293:1349	These essential pathways may serve as new targets in the drug development against S. pneumoniae.					
29398251	8	11	theme	essential	1261:1269	arg1	pathways					1271:1278	These essential pathways	1255:1278	These essential pathways	1255:1278	These essential pathways may serve as new targets in the drug development against S. pneumoniae.					
29398251	6	12	theme	intracellular	934:946	arg1	profile					958:964	The intracellular metabolic profile	930:964	The intracellular metabolic profile	930:964	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	7	13	dep	derived	1216:1222	arg1	host					1211:1214	host	1211:1214	host	1211:1214	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	6	14	theme	growing	1109:1115	arg1	cells					1117:1121	growing cells	1109:1121	growing cells	1109:1121	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	1	15	theme	diseases	178:185	arg1	pneumonia					197:205	pneumonia	197:205	pneumonia	197:205	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	1	15	theme	diseases	178:185	arg1	range					162:166	a broad range	154:166	a broad range of severe diseases including pneumonia and septicemia	154:220	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	1	15	theme	diseases	178:185	arg1	septicemia					211:220	septicemia	211:220	septicemia	211:220	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	5	16	theme	validated	775:783	arg1	protocol					785:792	our validated protocol	771:792	our validated protocol for the pneumococcal intracellular metabolome analysis	771:847	Employing our validated protocol for the pneumococcal intracellular metabolome analysis, a time resolved snapshot of the primary metabolism of pneumococci was obtained.					
29398251	8	17	from	targets	1297:1303	arg1	development					1317:1327	the drug development	1308:1327	the drug development against S. pneumoniae	1308:1349	These essential pathways may serve as new targets in the drug development against S. pneumoniae.					
29398251	5	18	theme	pneumococci	904:914	arg1	metabolism					890:899	the primary metabolism	878:899	the primary metabolism of pneumococci	878:914	Employing our validated protocol for the pneumococcal intracellular metabolome analysis, a time resolved snapshot of the primary metabolism of pneumococci was obtained.					
29398251	7	19	theme	biochemical	1158:1168	arg1	dependency					1170:1179	the biochemical dependency	1154:1179	the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides	1154:1252	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	0	20	theme	Metabolic	0:8	arg1	inventory					10:18	Metabolic inventory	0:18	Metabolic inventory of Streptococcus pneumoniae	0:46	Metabolic inventory of Streptococcus pneumoniae growing in a chemical defined environment.					
29398251	3	21	from	growth	512:517	arg1	medium					541:546	a chemical defined medium	522:546	a chemical defined medium composed of typical host metabolites	522:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	1	22	theme	Gram-positive	95:107	arg1	pneumoniae					133:142	The Gram-positive bacterium Streptococcus pneumoniae	91:142	The Gram-positive bacterium Streptococcus pneumoniae	91:142	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	0	23	theme	Streptococcus	23:35	arg1	pneumoniae					37:46	Streptococcus pneumoniae	23:46	Streptococcus pneumoniae	23:46	Metabolic inventory of Streptococcus pneumoniae growing in a chemical defined environment.					
29398251	7	24	from	dependency	1170:1179	arg1	nutrients					1224:1232	external host derived nutrients	1202:1232	external host derived nutrients such as nucleosides	1202:1252	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	7	24	from	dependency	1170:1179	arg1	nucleosides					1242:1252	nucleosides	1242:1252	nucleosides	1242:1252	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	2	25	theme	amino	324:328	arg1	acids					330:334	amino acids	324:334	amino acids	324:334	The pneumococcal pathophysiology is highly dependent on host nutrients such as purines, pyrimidines, amino acids and carbon sources.					
29398251	3	26	theme	basic	473:477	arg1	characteristics					489:503	the basic metabolic characteristics	469:503	the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites	469:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	6	27	theme	concentrated	1017:1028	arg1	precursors					1030:1039	high concentrated precursors	1012:1039	high concentrated precursors of peptidoglycan synthesis	1012:1066	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	4	28	dep	1H-NMR	612:617	arg1	methods					638:644	methods	638:644	methods	638:644	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	1	29	theme	Streptococcus	119:131	arg1	pneumoniae					133:142	The Gram-positive bacterium Streptococcus pneumoniae	91:142	The Gram-positive bacterium Streptococcus pneumoniae	91:142	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	3	30	theme	metabolic	479:487	arg1	characteristics					489:503	the basic metabolic characteristics	469:503	the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites	469:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	5	31	theme	intracellular	815:827	arg1	analysis					840:847	the pneumococcal intracellular metabolome analysis	798:847	the pneumococcal intracellular metabolome analysis	798:847	Employing our validated protocol for the pneumococcal intracellular metabolome analysis, a time resolved snapshot of the primary metabolism of pneumococci was obtained.					
29398251	6	32	theme	high	1012:1015	arg1	precursors					1030:1039	high concentrated precursors	1012:1039	high concentrated precursors of peptidoglycan synthesis	1012:1066	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	5	33	theme	primary	882:888	arg1	metabolism					890:899	the primary metabolism	878:899	the primary metabolism of pneumococci	878:914	Employing our validated protocol for the pneumococcal intracellular metabolome analysis, a time resolved snapshot of the primary metabolism of pneumococci was obtained.					
29398251	7	34	link	derived	1216:1222	arg1	nutrients					1224:1232	external host derived nutrients	1202:1232	external host derived nutrients such as nucleosides	1202:1252	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	7	34	link	derived	1216:1222	arg1	nucleosides					1242:1252	nucleosides	1242:1252	nucleosides	1242:1252	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	4	35	theme	extracellular	659:671	arg1	uptake					673:678	extracellular uptake	659:678	extracellular uptake	659:678	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	0	36	theme	pneumoniae	37:46	arg1	inventory					10:18	Metabolic inventory	0:18	Metabolic inventory of Streptococcus pneumoniae	0:46	Metabolic inventory of Streptococcus pneumoniae growing in a chemical defined environment.					
29398251	3	37	with	metabolome	392:401	arg1	approach					423:430	a metabolomics approach	408:430	a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites	408:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	5	38	theme	metabolism	890:899	arg1	snapshot					866:873	snapshot	866:873	snapshot of the primary metabolism of pneumococci	866:914	Employing our validated protocol for the pneumococcal intracellular metabolome analysis, a time resolved snapshot of the primary metabolism of pneumococci was obtained.					
29398251	7	39	theme	derived	1216:1222	arg1	nutrients					1224:1232	external host derived nutrients	1202:1232	external host derived nutrients such as nucleosides	1202:1252	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	7	39	theme	derived	1216:1222	arg1	nucleosides					1242:1252	nucleosides	1242:1252	nucleosides	1242:1252	Furthermore, our data reflect the biochemical dependency for S. pneumoniae on external host derived nutrients such as nucleosides.					
29398251	8	40	theme	new	1293:1295	arg1	targets					1297:1303	new targets	1293:1303	new targets in the drug development against S. pneumoniae	1293:1349	These essential pathways may serve as new targets in the drug development against S. pneumoniae.					
29398251	8	40	theme	new	1293:1295	arg1	pathways					1271:1278	These essential pathways	1255:1278	These essential pathways	1255:1278	These essential pathways may serve as new targets in the drug development against S. pneumoniae.					
29398251	4	41	theme	intracellular	724:736	arg1	composition					748:758	the intracellular metabolic composition	720:758	metabolites as well as the intracellular metabolic composition	697:758	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	3	42	theme	metabolomics	410:421	arg1	approach					423:430	a metabolomics approach	408:430	a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites	408:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	2	43	theme	pneumococcal	227:238	arg1	pathophysiology					240:254	The pneumococcal pathophysiology	223:254	The pneumococcal pathophysiology	223:254	The pneumococcal pathophysiology is highly dependent on host nutrients such as purines, pyrimidines, amino acids and carbon sources.					
29398251	2	43	theme	pneumococcal	227:238	arg1	dependent					266:274	dependent	266:274	dependent	266:274	The pneumococcal pathophysiology is highly dependent on host nutrients such as purines, pyrimidines, amino acids and carbon sources.					
29398251	6	44	theme	synthesis	1058:1066	arg1	precursors					1030:1039	high concentrated precursors	1012:1039	high concentrated precursors of peptidoglycan synthesis	1012:1066	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	6	45	theme	glycolytic	983:992	arg1	flux					994:997	a high glycolytic flux	976:997	a high glycolytic flux	976:997	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	1	46	theme	broad	156:160	arg1	pneumonia					197:205	pneumonia	197:205	pneumonia	197:205	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	1	46	theme	broad	156:160	arg1	range					162:166	a broad range	154:166	a broad range of severe diseases including pneumonia and septicemia	154:220	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	1	46	theme	broad	156:160	arg1	septicemia					211:220	septicemia	211:220	septicemia	211:220	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	3	47	theme	characteristics	489:503	arg1	investigation					452:464	the investigation	448:464	the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites	448:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	1	48	theme	bacterium	109:117	arg1	pneumoniae					133:142	The Gram-positive bacterium Streptococcus pneumoniae	91:142	The Gram-positive bacterium Streptococcus pneumoniae	91:142	The Gram-positive bacterium Streptococcus pneumoniae can cause a broad range of severe diseases including pneumonia and septicemia.					
29398251	4	49	theme	HPLC-MS	620:626	arg1	combination					597:607	a combination	595:607	a combination of 1H-NMR, HPLC-MS and GC-MS methods	595:644	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	2	50	theme	carbon	340:345	arg1	sources					347:353	carbon sources	340:353	carbon sources	340:353	The pneumococcal pathophysiology is highly dependent on host nutrients such as purines, pyrimidines, amino acids and carbon sources.					
29398251	4	51	theme	composition	748:758	arg1	secretion					684:692	secretion	684:692	secretion of metabolites as well as the intracellular metabolic composition	684:758	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	4	51	theme	composition	748:758	arg1	uptake					673:678	extracellular uptake	659:678	extracellular uptake	659:678	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	0	52	theme	defined	70:76	arg1	environment					78:88	a chemical defined environment	59:88	a chemical defined environment	59:88	Metabolic inventory of Streptococcus pneumoniae growing in a chemical defined environment.					
29398251	2	53	theme	host	279:282	arg1	acids					330:334	amino acids	324:334	amino acids	324:334	The pneumococcal pathophysiology is highly dependent on host nutrients such as purines, pyrimidines, amino acids and carbon sources.					
29398251	2	53	theme	host	279:282	arg1	pyrimidines					311:321	pyrimidines	311:321	pyrimidines	311:321	The pneumococcal pathophysiology is highly dependent on host nutrients such as purines, pyrimidines, amino acids and carbon sources.					
29398251	2	53	theme	host	279:282	arg1	nutrients					284:292	host nutrients	279:292	host nutrients such as purines, pyrimidines, amino acids and carbon sources	279:353	The pneumococcal pathophysiology is highly dependent on host nutrients such as purines, pyrimidines, amino acids and carbon sources.					
29398251	2	53	theme	host	279:282	arg1	sources					347:353	carbon sources	340:353	carbon sources	340:353	The pneumococcal pathophysiology is highly dependent on host nutrients such as purines, pyrimidines, amino acids and carbon sources.					
29398251	2	53	theme	host	279:282	arg1	purines					302:308	purines	302:308	purines	302:308	The pneumococcal pathophysiology is highly dependent on host nutrients such as purines, pyrimidines, amino acids and carbon sources.					
29398251	3	54	theme	defined	533:539	arg1	medium					541:546	a chemical defined medium	522:546	a chemical defined medium composed of typical host metabolites	522:583	Therefore, we aimed to decipher the metabolome with a metabolomics approach that allows for the investigation of the basic metabolic characteristics during growth in a chemical defined medium composed of typical host metabolites.					
29398251	8	55	theme	drug	1312:1315	arg1	development					1317:1327	the drug development	1308:1327	the drug development against S. pneumoniae	1308:1349	These essential pathways may serve as new targets in the drug development against S. pneumoniae.					
29398251	4	56	theme	GC-MS	632:636	arg1	combination					597:607	a combination	595:607	a combination of 1H-NMR, HPLC-MS and GC-MS methods	595:644	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	0	57	theme	chemical	61:68	arg1	environment					78:88	a chemical defined environment	59:88	a chemical defined environment	59:88	Metabolic inventory of Streptococcus pneumoniae growing in a chemical defined environment.					
29398251	6	58	theme	high	978:981	arg1	flux					994:997	a high glycolytic flux	976:997	a high glycolytic flux	976:997	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
29398251	5	59	theme	metabolome	829:838	arg1	analysis					840:847	the pneumococcal intracellular metabolome analysis	798:847	the pneumococcal intracellular metabolome analysis	798:847	Employing our validated protocol for the pneumococcal intracellular metabolome analysis, a time resolved snapshot of the primary metabolism of pneumococci was obtained.					
29398251	4	60	theme	metabolites	697:707	arg1	secretion					684:692	secretion	684:692	secretion of metabolites as well as the intracellular metabolic composition	684:758	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	4	60	theme	metabolites	697:707	arg1	uptake					673:678	extracellular uptake	659:678	extracellular uptake	659:678	By using a combination of 1H-NMR, HPLC-MS and GC-MS methods we monitored extracellular uptake and secretion of metabolites as well as the intracellular metabolic composition.					
29398251	6	61	theme	fuel	1080:1083	arg1	cell-wall-metabolism					1085:1104	fuel cell-wall-metabolism	1080:1104	fuel cell-wall-metabolism in growing cells	1080:1121	The intracellular metabolic profile indicates a high glycolytic flux and displays high concentrated precursors of peptidoglycan synthesis probably to fuel cell-wall-metabolism in growing cells.					
31499960	5	0	theme	transient	831:839	arg1	interactions					841:852	transient interactions	831:852	transient interactions	831:852	The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions.					
31499960	1	1	theme	implantable	147:157	arg1	devices					159:165	the implantable devices	143:165	the implantable devices	143:165	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	5	2	theme	film	688:691	arg1	composition					693:703	The film composition	684:703	The film composition	684:703	The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions.					
31499960	1	3	theme	devices	159:165	arg1	Surface					132:138	Surface	132:138	Surface of the implantable devices	132:165	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	1	3	theme	devices	159:165	arg1	cause					179:183	the root cause	170:183	the root cause of several complications such as infections, implant loosening and chronic inflammation	170:271	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	3	4	link	derived	522:528	arg1	polypeptide					530:540	a naturally derived polypeptide	510:540	a naturally derived polypeptide	510:540	Herein, we developed a coating system composed of ECM components and a naturally derived polypeptide.					
31499960	7	5	theme	endothelial	1125:1135	arg1	PECAM					1157:1161	PECAM	1157:1161	PECAM	1157:1161	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	7	5	theme	endothelial	1125:1135	arg1	contacts					1147:1154	endothelial cell-cell contacts	1125:1154	endothelial cell-cell contacts (PECAM)	1125:1162	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	1	6	theme	chronic	252:258	arg1	inflammation					260:271	chronic inflammation	252:271	chronic inflammation	252:271	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	0	7	theme	chain	92:96	arg1	arginine					121:128	arginine	121:128	arginine	121:128	Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine).					
31499960	0	7	theme	chain	92:96	arg1	poly					116:119	chain length-controlled poly	92:119	chain length-controlled poly(arginine)	92:129	Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine).					
31499960	6	8	theme	PAR	927:929	arg1	loading					916:922	a significantly higher loading	893:922	a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure	893:1002	Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure.					
31499960	7	9	theme	cell-cell	1137:1145	arg1	PECAM					1157:1161	PECAM	1157:1161	PECAM	1157:1161	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	7	9	theme	cell-cell	1137:1145	arg1	contacts					1147:1154	endothelial cell-cell contacts	1125:1154	endothelial cell-cell contacts (PECAM)	1125:1162	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	8	10	theme	implants	1306:1313	arg1	surface					1295:1301	surface	1295:1301	surface of implants	1295:1313	This multifunctional coating can be easily applied to surface of implants where it can enact on several problems simultaneously.					
31499960	5	11	dep	based	708:712	arg1	modified					742:749	modified	742:749	modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions	742:852	The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions.					
31499960	3	12	theme	coating	464:470	arg1	system					472:477	a coating system	462:477	a coating system composed of ECM components and a naturally derived polypeptide	462:540	Herein, we developed a coating system composed of ECM components and a naturally derived polypeptide.					
31499960	3	13	theme	derived	522:528	arg1	polypeptide					530:540	a naturally derived polypeptide	510:540	a naturally derived polypeptide	510:540	Herein, we developed a coating system composed of ECM components and a naturally derived polypeptide.					
31499960	6	14	contain	containing	940:949	arg2	HA-Ald					951:956	HA-Ald	951:956	HA-Ald	951:956	Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure.					
31499960	6	14	contain	containing	940:949	arg1	films					934:938	films	934:938	films containing HA-Ald with longer retention of PAR in the structure	934:1002	Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure.					
31499960	7	15	theme	human	1207:1211	arg1	cells					1234:1238	human vascular endothelial cells	1207:1238	human vascular endothelial cells	1207:1238	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	2	16	theme	similar	390:396	arg1	manner					383:388	a manner	381:388	a manner similar to the structures of extracellular matrix	381:438	There is an urgent need for multifunctional coatings that can address these shortcomings simultaneously in a manner similar to the structures of extracellular matrix.					
31499960	0	17	theme	polymeric	16:24	arg1	coatings					34:41	Multifunctional polymeric implant coatings	0:41	Multifunctional polymeric implant coatings	0:41	Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine).					
31499960	6	18	theme	higher	909:914	arg1	loading					916:922	a significantly higher loading	893:922	a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure	893:1002	Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure.					
31499960	7	19	theme	antimicrobial	1063:1075	arg1	properties					1095:1104	the film surface antimicrobial (contact-killing) properties	1046:1104	the film surface antimicrobial (contact-killing) properties	1046:1104	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	2	20	theme	multifunctional	302:316	arg1	coatings					318:325	multifunctional coatings	302:325	multifunctional coatings that can address these shortcomings simultaneously in a manner similar to the structures of extracellular matrix	302:438	There is an urgent need for multifunctional coatings that can address these shortcomings simultaneously in a manner similar to the structures of extracellular matrix.					
31499960	6	21	from	retention	970:978	arg1	structure					994:1002	the structure	990:1002	the structure	990:1002	Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure.					
31499960	1	22	theme	root	174:177	arg1	cause					179:183	the root cause	170:183	the root cause of several complications such as infections, implant loosening and chronic inflammation	170:271	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	1	22	theme	root	174:177	arg1	Surface					132:138	Surface	132:138	Surface of the implantable devices	132:165	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	0	23	theme	Multifunctional	0:14	arg1	coatings					34:41	Multifunctional polymeric implant coatings	0:41	Multifunctional polymeric implant coatings	0:41	Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine).					
31499960	6	24	from	loading	916:922	arg1	films					934:938	films	934:938	films containing HA-Ald with longer retention of PAR in the structure	934:1002	Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure.					
31499960	7	25	theme	gene	1174:1177	arg1	expression					1179:1188	VEGFA gene expression	1168:1188	VEGFA gene expression	1168:1188	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	7	26	theme	contact-killing	1078:1092	arg1	properties					1095:1104	the film surface antimicrobial (contact-killing) properties	1046:1104	the film surface antimicrobial (contact-killing) properties	1046:1104	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	8	27	theme	several	1337:1343	arg1	problems					1345:1352	several problems	1337:1352	several problems	1337:1352	This multifunctional coating can be easily applied to surface of implants where it can enact on several problems simultaneously.					
31499960	2	28	theme	extracellular	419:431	arg1	matrix					433:438	extracellular matrix	419:438	extracellular matrix	419:438	There is an urgent need for multifunctional coatings that can address these shortcomings simultaneously in a manner similar to the structures of extracellular matrix.					
31499960	2	29	theme	matrix	433:438	arg1	structures					405:414	the structures	401:414	the structures of extracellular matrix	401:438	There is an urgent need for multifunctional coatings that can address these shortcomings simultaneously in a manner similar to the structures of extracellular matrix.					
31499960	4	30	theme	coating	572:578	arg1	components					580:589	the coating components	568:589	the coating components	568:589	The interactions between the coating components create an environment that enables incorporation of an antimicrobial/angiogenic polypeptide.					
31499960	2	31	theme	urgent	286:291	arg1	need					293:296	an urgent need	283:296	an urgent need for multifunctional coatings that can address these shortcomings simultaneously in a manner similar to the structures of extracellular matrix	283:438	There is an urgent need for multifunctional coatings that can address these shortcomings simultaneously in a manner similar to the structures of extracellular matrix.					
31499960	1	32	theme	several	188:194	arg1	complications					196:208	several complications	188:208	several complications such as infections, implant loosening and chronic inflammation	188:271	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	1	32	theme	several	188:194	arg1	infections					218:227	infections	218:227	infections	218:227	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	1	32	theme	several	188:194	arg1	loosening					238:246	implant loosening	230:246	implant loosening	230:246	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	1	32	theme	several	188:194	arg1	inflammation					260:271	chronic inflammation	252:271	chronic inflammation	252:271	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	7	33	theme	vascular	1213:1220	arg1	cells					1234:1238	human vascular endothelial cells	1207:1238	human vascular endothelial cells	1207:1238	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	6	34	theme	PAR	983:985	arg1	retention					970:978	longer retention	963:978	longer retention of PAR in the structure	963:1002	Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure.					
31499960	5	35	mod	modified	742:749	arg3	groups					765:770	aldehyde groups	756:770	aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions	756:852	The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions.					
31499960	5	35	mod	modified	742:749	arg3	HA-Ald					773:778	HA-Ald	773:778	HA-Ald	773:778	The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions.					
31499960	5	35	mod	modified	742:749	arg1	composition					693:703	The film composition	684:703	The film composition	684:703	The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions.					
31499960	1	36	theme	complications	196:208	arg1	cause					179:183	the root cause	170:183	the root cause of several complications such as infections, implant loosening and chronic inflammation	170:271	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	1	36	theme	complications	196:208	arg1	Surface					132:138	Surface	132:138	Surface of the implantable devices	132:165	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31499960	7	37	theme	VEGFA	1168:1172	arg1	expression					1179:1188	VEGFA gene expression	1168:1188	VEGFA gene expression	1168:1188	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	5	38	theme	aldehyde	756:763	arg1	HA-Ald					773:778	HA-Ald	773:778	HA-Ald	773:778	The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions.					
31499960	5	38	theme	aldehyde	756:763	arg1	groups					765:770	aldehyde groups	756:770	aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions	756:852	The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions.					
31499960	4	39	theme	polypeptide	671:681	arg1	incorporation					626:638	incorporation	626:638	incorporation of an antimicrobial/angiogenic polypeptide	626:681	The interactions between the coating components create an environment that enables incorporation of an antimicrobial/angiogenic polypeptide.					
31499960	8	40	theme	multifunctional	1246:1260	arg1	coating					1262:1268	This multifunctional coating	1241:1268	This multifunctional coating	1241:1268	This multifunctional coating can be easily applied to surface of implants where it can enact on several problems simultaneously.					
31499960	0	41	theme	length-controlled	98:114	arg1	arginine					121:128	arginine	121:128	arginine	121:128	Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine).					
31499960	0	41	theme	length-controlled	98:114	arg1	poly					116:119	chain length-controlled poly	92:119	chain length-controlled poly(arginine)	92:129	Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine).					
31499960	7	42	theme	film	1050:1053	arg1	properties					1095:1104	the film surface antimicrobial (contact-killing) properties	1046:1104	the film surface antimicrobial (contact-killing) properties	1046:1104	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	7	43	theme	endothelial	1222:1232	arg1	cells					1234:1238	human vascular endothelial cells	1207:1238	human vascular endothelial cells	1207:1238	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	6	44	theme	longer	963:968	arg1	retention					970:978	longer retention	963:978	longer retention of PAR in the structure	963:1002	Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure.					
31499960	7	45	theme	surface	1055:1061	arg1	properties					1095:1104	the film surface antimicrobial (contact-killing) properties	1046:1104	the film surface antimicrobial (contact-killing) properties	1046:1104	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	6	46	theme	Nanoplasmon	855:865	arg1	measurements					867:878	Nanoplasmon measurements	855:878	Nanoplasmon measurements	855:878	Nanoplasmon measurements demonstrated a significantly higher loading of PAR in films containing HA-Ald with longer retention of PAR in the structure.					
31499960	7	47	theme	PAR	1021:1023	arg1	presence					1009:1016	The presence	1005:1016	The presence of PAR	1005:1023	The presence of PAR not only provides to the film surface antimicrobial (contact-killing) properties but also increased endothelial cell-cell contacts (PECAM) and VEGFA gene expression and secretion by human vascular endothelial cells.					
31499960	3	48	theme	ECM	491:493	arg1	components					495:504	ECM components	491:504	ECM components	491:504	Herein, we developed a coating system composed of ECM components and a naturally derived polypeptide.					
31499960	5	49	theme	hyaluronic	726:735	arg1	acid					737:740	hyaluronic acid	726:740	hyaluronic acid	726:740	The film composition is based gelatin and hyaluronic acid modified with aldehyde groups (HA-Ald) that can react with poly (arginine) (PAR) through transient interactions.					
31499960	0	50	theme	acid	72:75	arg1	derivative					77:86	hyaluronic acid derivative	61:86	hyaluronic acid derivative	61:86	Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine).					
31499960	4	51	theme	antimicrobial/angiogenic	646:669	arg1	polypeptide					671:681	an antimicrobial/angiogenic polypeptide	643:681	an antimicrobial/angiogenic polypeptide	643:681	The interactions between the coating components create an environment that enables incorporation of an antimicrobial/angiogenic polypeptide.					
31499960	0	52	theme	hyaluronic	61:70	arg1	derivative					77:86	hyaluronic acid derivative	61:86	hyaluronic acid derivative	61:86	Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine).					
31499960	0	53	theme	implant	26:32	arg1	coatings					34:41	Multifunctional polymeric implant coatings	0:41	Multifunctional polymeric implant coatings	0:41	Multifunctional polymeric implant coatings based on gelatin, hyaluronic acid derivative and chain length-controlled poly(arginine).					
31499960	1	54	theme	implant	230:236	arg1	loosening					238:246	implant loosening	230:246	implant loosening	230:246	Surface of the implantable devices is the root cause of several complications such as infections, implant loosening and chronic inflammation.					
31530627	5	0	theme	metabolite	701:710	arg1	levels					712:717	the intracellular metabolite levels	683:717	the intracellular metabolite levels	683:717	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	7	1	theme	provision	1085:1093	arg1	terms					1046:1050	terms	1046:1050	terms of carbon, reductant, and energy provision	1046:1093	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	3	2	theme	elevated	440:447	arg1	content					453:459	elevated oil content	440:459	elevated oil content in maize	440:468	So far, breeding efforts to achieve elevated oil content in maize have resulted in smaller endosperms and therefore lower yield.					
31530627	8	3	theme	mathematical	1309:1320	arg1	modeling					1322:1329	mathematical modeling	1309:1329	mathematical modeling with biochemical quantification	1309:1361	This approach combined mathematical modeling with biochemical quantification to identify metabolic bottlenecks in FAS in maize embryos.					
31530627	7	4	from	FAS	1039:1041	arg1	terms					1046:1050	terms	1046:1050	terms of carbon, reductant, and energy provision	1046:1093	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	9	5	theme	important	1536:1544	arg1	crops					1551:1555	important food crops	1536:1555	important food crops	1536:1555	This study describes a combination of innovative tools that will pave the way for controlling seed composition in important food crops.					
31530627	6	6	from	embryos	961:967	arg1	active					947:952	active	947:952	active	947:952	Comparative metabolomics highlighted the metabolites and pathways that were active in the embryos and important for oil production.					
31530627	3	7	theme	oil	449:451	arg1	content					453:459	elevated oil content	440:459	elevated oil content in maize	440:468	So far, breeding efforts to achieve elevated oil content in maize have resulted in smaller endosperms and therefore lower yield.					
31530627	8	8	from	bottlenecks	1385:1395	arg1	FAS					1400:1402	FAS	1400:1402	FAS in maize embryos	1400:1419	This approach combined mathematical modeling with biochemical quantification to identify metabolic bottlenecks in FAS in maize embryos.					
31530627	6	9	theme	oil	987:989	arg1	production					991:1000	oil production	987:1000	oil production	987:1000	Comparative metabolomics highlighted the metabolites and pathways that were active in the embryos and important for oil production.					
31530627	9	10	theme	food	1546:1549	arg1	crops					1551:1555	important food crops	1536:1555	important food crops	1536:1555	This study describes a combination of innovative tools that will pave the way for controlling seed composition in important food crops.					
31530627	7	11	theme	flux	1182:1185	arg1	analysis					1187:1194	13C-metabolic flux analysis	1168:1194	13C-metabolic flux analysis	1168:1194	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	7	11	theme	flux	1182:1185	arg1	network					1159:1165	the metabolic network	1145:1165	the metabolic network (13C-metabolic flux analysis)	1145:1195	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	6	12	theme	Comparative	871:881	arg1	metabolomics					883:894	Comparative metabolomics	871:894	Comparative metabolomics	871:894	Comparative metabolomics highlighted the metabolites and pathways that were active in the embryos and important for oil production.					
31530627	0	13	theme	Maize	90:94	arg1	Embryos					96:102	Maize Embryos	90:102	Maize Embryos	90:102	A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos.					
31530627	6	14	dep	metabolites	912:922	arg1	the					908:910	the	908:910	the	908:910	Comparative metabolomics highlighted the metabolites and pathways that were active in the embryos and important for oil production.					
31530627	5	15	theme	oil	852:854	arg1	oil					852:854	oil	852:854	oil	852:854	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	15	theme	oil	852:854	arg1	%					839:839	48%	837:839	48%	837:839	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	15	theme	oil	852:854	arg1	%					847:847	34%	845:847	34% of oil	845:854	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	3	16	from	content	453:459	arg1	maize					464:468	maize	464:468	maize	464:468	So far, breeding efforts to achieve elevated oil content in maize have resulted in smaller endosperms and therefore lower yield.					
31530627	3	17	dep	efforts	421:427	arg1	achieve					432:438	achieve	432:438	to achieve elevated oil content in maize	429:468	So far, breeding efforts to achieve elevated oil content in maize have resulted in smaller endosperms and therefore lower yield.					
31530627	4	18	from	content	608:614	arg1	kernels					625:631	maize kernels	619:631	maize kernels	619:631	Directly changing their carbon metabolism may be the key to increasing oil content in maize kernels without affecting yield.					
31530627	1	19	theme	growing	240:246	arg1	demand					248:253	the rapidly growing demand	228:253	the rapidly growing demand for vegetable oil	228:271	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	7	20	theme	reductant	1063:1071	arg1	terms					1046:1050	terms	1046:1050	terms of carbon, reductant, and energy provision	1046:1093	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	7	21	theme	carbon	1125:1130	arg1	flow					1132:1135	the carbon flow	1121:1135	the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism	1121:1283	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	9	22	from	composition	1521:1531	arg1	crops					1551:1555	important food crops	1536:1555	important food crops	1536:1555	This study describes a combination of innovative tools that will pave the way for controlling seed composition in important food crops.					
31530627	5	23	theme	S	789:789	arg1	Alex					804:807	ALEXHO S K SYNTHETIC (Alex)	782:808	ALEXHO S K SYNTHETIC (Alex)	782:808	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	23	theme	S	789:789	arg1	lines					775:779	two different maize lines	755:779	two different maize lines	755:779	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	1	24	from	synthesis	126:134	arg1	mays					156:159	Zea mays	152:159	Zea mays	152:159	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	24	from	synthesis	126:134	arg1	maize					145:149	maize	145:149	maize (Zea mays)	145:160	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	25	theme	Zea	152:154	arg1	mays					156:159	Zea mays	152:159	Zea mays	152:159	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	25	theme	Zea	152:154	arg1	maize					145:149	maize	145:149	maize (Zea mays)	145:160	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	0	26	theme	Combined	2:9	arg1	Metabolomics					11:22	A Combined Metabolomics and Fluxomics Analysis	0:45	Metabolomics	11:22	A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos.					
31530627	5	27	theme	ALEXHO	782:787	arg1	Alex					804:807	ALEXHO S K SYNTHETIC (Alex)	782:808	ALEXHO S K SYNTHETIC (Alex)	782:808	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	27	theme	ALEXHO	782:787	arg1	lines					775:779	two different maize lines	755:779	two different maize lines	755:779	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	7	28	theme	central	1266:1272	arg1	metabolism					1274:1283	the central metabolism	1262:1283	the central metabolism	1262:1283	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	8	29	theme	maize	1407:1411	arg1	embryos					1413:1419	maize embryos	1407:1419	maize embryos	1407:1419	This approach combined mathematical modeling with biochemical quantification to identify metabolic bottlenecks in FAS in maize embryos.					
31530627	5	30	theme	SYNTHETIC	793:801	arg1	Alex					804:807	ALEXHO S K SYNTHETIC (Alex)	782:808	ALEXHO S K SYNTHETIC (Alex)	782:808	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	30	theme	SYNTHETIC	793:801	arg1	lines					775:779	two different maize lines	755:779	two different maize lines	755:779	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	8	31	from	FAS	1400:1402	arg1	embryos					1413:1419	maize embryos	1407:1419	maize embryos	1407:1419	This approach combined mathematical modeling with biochemical quantification to identify metabolic bottlenecks in FAS in maize embryos.					
31530627	2	32	theme	maize	277:281	arg1	kernels					283:289	maize kernels	277:289	maize kernels	277:289	In maize kernels, the endosperm and the embryo are the main sites for synthesis and accumulation of starch and oil, respectively.					
31530627	9	33	theme	innovative	1460:1469	arg1	tools					1471:1475	innovative tools	1460:1475	innovative tools that will pave the way for controlling seed composition in important food crops	1460:1555	This study describes a combination of innovative tools that will pave the way for controlling seed composition in important food crops.					
31530627	2	34	theme	main	329:332	arg1	endosperm					296:304	the endosperm	292:304	the endosperm	292:304	In maize kernels, the endosperm and the embryo are the main sites for synthesis and accumulation of starch and oil, respectively.					
31530627	2	34	theme	main	329:332	arg1	embryo					314:319	the embryo	310:319	the embryo	310:319	In maize kernels, the endosperm and the embryo are the main sites for synthesis and accumulation of starch and oil, respectively.					
31530627	2	34	theme	main	329:332	arg1	sites					334:338	the main sites	325:338	the main sites for synthesis and accumulation of starch and oil, respectively	325:401	In maize kernels, the endosperm and the embryo are the main sites for synthesis and accumulation of starch and oil, respectively.					
31530627	2	35	theme	oil	385:387	arg1	accumulation					358:369	accumulation	358:369	accumulation of starch and oil, respectively	358:401	In maize kernels, the endosperm and the embryo are the main sites for synthesis and accumulation of starch and oil, respectively.					
31530627	2	35	theme	oil	385:387	arg1	synthesis					344:352	synthesis	344:352	synthesis	344:352	In maize kernels, the endosperm and the embryo are the main sites for synthesis and accumulation of starch and oil, respectively.					
31530627	6	36	from	active	947:952	arg1	embryos					961:967	the embryos	957:967	the embryos	957:967	Comparative metabolomics highlighted the metabolites and pathways that were active in the embryos and important for oil production.					
31530627	3	37	theme	smaller	487:493	arg1	endosperms					495:504	smaller endosperms	487:504	smaller endosperms	487:504	So far, breeding efforts to achieve elevated oil content in maize have resulted in smaller endosperms and therefore lower yield.					
31530627	2	38	theme	starch	374:379	arg1	accumulation					358:369	accumulation	358:369	accumulation of starch and oil, respectively	358:401	In maize kernels, the endosperm and the embryo are the main sites for synthesis and accumulation of starch and oil, respectively.					
31530627	2	38	theme	starch	374:379	arg1	synthesis					344:352	synthesis	344:352	synthesis	344:352	In maize kernels, the endosperm and the embryo are the main sites for synthesis and accumulation of starch and oil, respectively.					
31530627	5	39	theme	different	759:767	arg1	Alex					804:807	ALEXHO S K SYNTHETIC (Alex)	782:808	ALEXHO S K SYNTHETIC (Alex)	782:808	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	39	theme	different	759:767	arg1	LH59					814:817	LH59	814:817	LH59	814:817	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	39	theme	different	759:767	arg1	lines					775:779	two different maize lines	755:779	two different maize lines	755:779	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	1	40	theme	vegetable	259:267	arg1	oil					269:271	vegetable oil	259:271	vegetable oil	259:271	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	41	theme	Enhancing	105:113	arg1	synthesis					126:134	Enhancing fatty acid synthesis	105:134	Enhancing fatty acid synthesis (FAS) in maize (Zea mays)	105:160	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	41	theme	Enhancing	105:113	arg1	FAS					137:139	FAS	137:139	FAS	137:139	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	42	theme	tremendous	166:175	arg1	benefits					212:219	tremendous potential nutritional and economic benefits	166:219	tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil	166:271	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	7	43	theme	13C-metabolic	1168:1180	arg1	analysis					1187:1194	13C-metabolic flux analysis	1168:1194	13C-metabolic flux analysis	1168:1194	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	7	43	theme	13C-metabolic	1168:1180	arg1	network					1159:1165	the metabolic network	1145:1165	the metabolic network (13C-metabolic flux analysis)	1145:1195	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	0	44	from	Synthesis	77:85	arg1	Embryos					96:102	Maize Embryos	90:102	Maize Embryos	90:102	A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos.					
31530627	7	45	theme	carbon	1242:1247	arg1	flow					1249:1252	carbon flow	1242:1252	carbon flow through the central metabolism	1242:1283	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	4	46	theme	oil	604:606	arg1	content					608:614	oil content	604:614	oil content in maize kernels	604:631	Directly changing their carbon metabolism may be the key to increasing oil content in maize kernels without affecting yield.					
31530627	1	47	theme	potential	177:185	arg1	benefits					212:219	tremendous potential nutritional and economic benefits	166:219	tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil	166:271	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	0	48	theme	Fluxomics	28:36	arg1	Analysis					38:45	A Combined Metabolomics and Fluxomics Analysis	0:45	Analysis	38:45	A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos.					
31530627	8	49	theme	biochemical	1336:1346	arg1	quantification					1348:1361	biochemical quantification	1336:1361	biochemical quantification	1336:1361	This approach combined mathematical modeling with biochemical quantification to identify metabolic bottlenecks in FAS in maize embryos.					
31530627	7	50	theme	flow	1249:1252	arg1	map					1235:1237	a map	1233:1237	a map of carbon flow through the central metabolism	1233:1283	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	2	51	from	sites	334:338	arg1	kernels					283:289	maize kernels	277:289	maize kernels	277:289	In maize kernels, the endosperm and the embryo are the main sites for synthesis and accumulation of starch and oil, respectively.					
31530627	1	52	theme	nutritional	187:197	arg1	benefits					212:219	tremendous potential nutritional and economic benefits	166:219	tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil	166:271	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	7	53	theme	pathway	1028:1034	arg1	contribution					1007:1018	The contribution	1003:1018	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision	1003:1093	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	7	54	theme	energy	1078:1083	arg1	provision					1085:1093	energy provision	1078:1093	energy provision	1078:1093	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	3	55	theme	lower	520:524	arg1	yield					526:530	therefore lower yield	510:530	therefore lower yield	510:530	So far, breeding efforts to achieve elevated oil content in maize have resulted in smaller endosperms and therefore lower yield.					
31530627	1	56	theme	fatty	115:119	arg1	synthesis					126:134	Enhancing fatty acid synthesis	105:134	Enhancing fatty acid synthesis (FAS) in maize (Zea mays)	105:160	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	56	theme	fatty	115:119	arg1	FAS					137:139	FAS	137:139	FAS	137:139	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	5	57	theme	maize	736:740	arg1	embryos					742:748	maize embryos	736:748	maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively	736:868	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	7	58	theme	Alex	1211:1214	arg1	embryos					1216:1222	Alex embryos	1211:1222	Alex embryos	1211:1222	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	1	59	theme	acid	121:124	arg1	synthesis					126:134	Enhancing fatty acid synthesis	105:134	Enhancing fatty acid synthesis (FAS) in maize (Zea mays)	105:160	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	59	theme	acid	121:124	arg1	FAS					137:139	FAS	137:139	FAS	137:139	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	8	60	with	modeling	1322:1329	arg1	quantification					1348:1361	biochemical quantification	1336:1361	biochemical quantification	1336:1361	This approach combined mathematical modeling with biochemical quantification to identify metabolic bottlenecks in FAS in maize embryos.					
31530627	5	61	from	lines	775:779	arg1	embryos					742:748	maize embryos	736:748	maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively	736:868	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	3	62	theme	breeding	412:419	arg1	efforts					421:427	breeding efforts	412:427	breeding efforts to achieve elevated oil content in maize	412:468	So far, breeding efforts to achieve elevated oil content in maize have resulted in smaller endosperms and therefore lower yield.					
31530627	9	63	theme	tools	1471:1475	arg1	combination					1445:1455	a combination	1443:1455	a combination of innovative tools that will pave the way for controlling seed composition in important food crops	1443:1555	This study describes a combination of innovative tools that will pave the way for controlling seed composition in important food crops.					
31530627	7	64	theme	metabolic	1149:1157	arg1	analysis					1187:1194	13C-metabolic flux analysis	1168:1194	13C-metabolic flux analysis	1168:1194	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	7	64	theme	metabolic	1149:1157	arg1	network					1159:1165	the metabolic network	1145:1165	the metabolic network (13C-metabolic flux analysis)	1145:1195	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	8	65	theme	metabolic	1375:1383	arg1	bottlenecks					1385:1395	metabolic bottlenecks	1375:1395	metabolic bottlenecks in FAS in maize embryos	1375:1419	This approach combined mathematical modeling with biochemical quantification to identify metabolic bottlenecks in FAS in maize embryos.					
31530627	1	66	theme	economic	203:210	arg1	benefits					212:219	tremendous potential nutritional and economic benefits	166:219	tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil	166:271	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	0	67	theme	Oil	73:75	arg1	Synthesis					77:85	Oil Synthesis	73:85	Oil Synthesis in Maize Embryos	73:102	A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos.					
31530627	1	68	contain	has	162:164	arg1	synthesis					126:134	Enhancing fatty acid synthesis	105:134	Enhancing fatty acid synthesis (FAS) in maize (Zea mays)	105:160	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	68	contain	has	162:164	arg1	FAS					137:139	FAS	137:139	FAS	137:139	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	1	68	contain	has	162:164	arg2	benefits					212:219	tremendous potential nutritional and economic benefits	166:219	tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil	166:271	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
31530627	9	69	theme	seed	1516:1519	arg1	composition					1521:1531	seed composition	1516:1531	seed composition in important food crops	1516:1555	This study describes a combination of innovative tools that will pave the way for controlling seed composition in important food crops.					
31530627	5	70	theme	maize	769:773	arg1	Alex					804:807	ALEXHO S K SYNTHETIC (Alex)	782:808	ALEXHO S K SYNTHETIC (Alex)	782:808	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	70	theme	maize	769:773	arg1	LH59					814:817	LH59	814:817	LH59	814:817	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	70	theme	maize	769:773	arg1	lines					775:779	two different maize lines	755:779	two different maize lines	755:779	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	4	71	theme	maize	619:623	arg1	kernels					625:631	maize kernels	619:631	maize kernels	619:631	Directly changing their carbon metabolism may be the key to increasing oil content in maize kernels without affecting yield.					
31530627	5	72	theme	K	791:791	arg1	Alex					804:807	ALEXHO S K SYNTHETIC (Alex)	782:808	ALEXHO S K SYNTHETIC (Alex)	782:808	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	72	theme	K	791:791	arg1	lines					775:779	two different maize lines	755:779	two different maize lines	755:779	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	5	73	theme	intracellular	687:699	arg1	levels					712:717	the intracellular metabolite levels	683:717	the intracellular metabolite levels	683:717	To test this hypothesis, the intracellular metabolite levels were compared in maize embryos from two different maize lines, ALEXHO S K SYNTHETIC (Alex) and LH59, which accumulate 48% and 34% of oil, respectively.					
31530627	4	74	theme	carbon	557:562	arg1	metabolism					564:573	their carbon metabolism	551:573	their carbon metabolism	551:573	Directly changing their carbon metabolism may be the key to increasing oil content in maize kernels without affecting yield.					
31530627	7	75	theme	carbon	1055:1060	arg1	terms					1046:1050	terms	1046:1050	terms of carbon, reductant, and energy provision	1046:1093	The contribution of each pathway to FAS in terms of carbon, reductant, and energy provision was assessed by measuring the carbon flow through the metabolic network (13C-metabolic flux analysis) in developing Alex embryos to build a map of carbon flow through the central metabolism.					
31530627	1	76	theme	due	221:223	arg1	benefits					212:219	tremendous potential nutritional and economic benefits	166:219	tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil	166:271	Enhancing fatty acid synthesis (FAS) in maize (Zea mays) has tremendous potential nutritional and economic benefits due to the rapidly growing demand for vegetable oil.					
30031085	5	0	with	ascorbate	890:898	arg1	ascorbate					938:946	calcium ascorbate	930:946	calcium ascorbate (A3)	930:951	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	5	0	with	ascorbate	890:898	arg1	A3					949:950	A3	949:950	A3	949:950	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	9	1	with	way	1395:1397	arg1	application					1479:1489	medical application	1471:1489	medical application	1471:1489	The results found in this study open an important way for development of new biomaterials, economically competitive, and with medical application.					
30031085	1	2	theme	versatile	166:174	arg1	platforms					183:191	versatile active platforms	166:191	versatile active platforms	166:191	New nanoparticles containing biomaterials are emerging as versatile active platforms in a great number of applications, for example, as skin substitutes and therapeutic media.					
30031085	8	3	dep	presented	1295:1303	arg1	A3					1292:1293	A3	1292:1293	A3	1292:1293	In addition, the antimicrobial analysis showed a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus.					
30031085	1	4	contain	containing	126:135	arg1	nanoparticles					112:124	New nanoparticles	108:124	New nanoparticles containing biomaterials	108:148	New nanoparticles containing biomaterials are emerging as versatile active platforms in a great number of applications, for example, as skin substitutes and therapeutic media.					
30031085	1	4	contain	containing	126:135	arg2	biomaterials					137:148	biomaterials	137:148	biomaterials	137:148	New nanoparticles containing biomaterials are emerging as versatile active platforms in a great number of applications, for example, as skin substitutes and therapeutic media.					
30031085	1	5	theme	active	176:181	arg1	platforms					183:191	versatile active platforms	166:191	versatile active platforms	166:191	New nanoparticles containing biomaterials are emerging as versatile active platforms in a great number of applications, for example, as skin substitutes and therapeutic media.					
30031085	4	6	theme	calcium	718:724	arg1	ascorbate					726:734	AgNPs were calcium ascorbate	707:734	AgNPs were calcium ascorbate	707:734	AgNPs synthetized using NaBH4 showed sizes ranging from 5 to 18 nm while AgNPs were calcium ascorbate was used showed micrometer from 164 to 955 nm size.					
30031085	9	7	located	found	1357:1361	arg1	study					1371:1375	this study	1366:1375	this study	1366:1375	The results found in this study open an important way for development of new biomaterials, economically competitive, and with medical application.					
30031085	9	7	located	found	1357:1361	arg2	results					1349:1355	The results	1345:1355	The results found in this study	1345:1375	The results found in this study open an important way for development of new biomaterials, economically competitive, and with medical application.					
30031085	2	8	theme	present	288:294	arg1	study					296:300	The present study	284:300	The present study	284:300	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	5	9	dep	formulations	817:828	arg1	agarose + AgNPs					858:872	agarose + AgNPs	858:872	three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3)	811:951	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	5	9	dep	formulations	817:828	arg1	film					847:850	agarose control film	831:850	three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3)	811:951	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	5	9	dep	formulations	817:828	arg1	A1					853:854	A1	853:854	A1	853:854	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	3	10	dep	applying	613:620	arg1	ii					609:610	ii	609:610	ii	609:610	Colloidal AgNPs were synthetized by two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4.					
30031085	0	11	theme	film	91:94	arg1	properties					96:105	film properties	91:105	film properties	91:105	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.					
30031085	6	12	theme	ascorbate	1055:1063	arg1	crystals					1065:1072	unreacted calcium ascorbate crystals	1037:1072	unreacted calcium ascorbate crystals	1037:1072	The characterization of films by SEM and EDS showed agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3.					
30031085	8	13	theme	particles	1226:1234	arg1	dependence					1244:1253	a silver particles release dependence	1217:1253	a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus	1217:1342	In addition, the antimicrobial analysis showed a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus.					
30031085	1	14	theme	therapeutic	265:275	arg1	media					277:281	therapeutic media	265:281	therapeutic media	265:281	New nanoparticles containing biomaterials are emerging as versatile active platforms in a great number of applications, for example, as skin substitutes and therapeutic media.					
30031085	4	15	theme	955 nm	775:780	arg1	size					782:785	955 nm size	775:785	955 nm size	775:785	AgNPs synthetized using NaBH4 showed sizes ranging from 5 to 18 nm while AgNPs were calcium ascorbate was used showed micrometer from 164 to 955 nm size.					
30031085	3	16	dep	applying	567:574	arg1	i					564:564	i	564:564	i	564:564	Colloidal AgNPs were synthetized by two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4.					
30031085	3	17	theme	Colloidal	496:504	arg1	AgNPs					506:510	Colloidal AgNPs	496:510	Colloidal AgNPs	496:510	Colloidal AgNPs were synthetized by two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4.					
30031085	3	18	dep	routes	555:560	arg1	applying					567:574	applying	567:574	applying calcium ascorbate and NaBH4	567:602	Colloidal AgNPs were synthetized by two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4.					
30031085	3	18	dep	routes	555:560	arg1	applying					613:620	applying	613:620	(ii) applying only NaBH4	608:631	Colloidal AgNPs were synthetized by two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4.					
30031085	0	19	theme	calcium	42:48	arg1	ascorbate					50:58	calcium ascorbate	42:58	calcium ascorbate	42:58	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.					
30031085	5	20	theme	agarose	831:837	arg1	film					847:850	agarose control film	831:850	three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3)	811:951	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	5	20	theme	agarose	831:837	arg1	A1					853:854	A1	853:854	A1	853:854	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	4	21	theme	AgNPs	707:711	arg1	ascorbate					726:734	AgNPs were calcium ascorbate	707:734	AgNPs were calcium ascorbate	707:734	AgNPs synthetized using NaBH4 showed sizes ranging from 5 to 18 nm while AgNPs were calcium ascorbate was used showed micrometer from 164 to 955 nm size.					
30031085	0	22	theme	particles	15:23	arg1	films					25:29	Agarose-silver particles films	0:29	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.	0:106	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.					
30031085	0	23	from	Effect	32:37	arg1	nanoparticles					63:75	nanoparticles synthesis and film properties	63:105	nanoparticles synthesis and film properties	63:105	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.					
30031085	9	24	theme	new	1418:1420	arg1	biomaterials					1422:1433	new biomaterials	1418:1433	new biomaterials	1418:1433	The results found in this study open an important way for development of new biomaterials, economically competitive, and with medical application.					
30031085	7	25	theme	calcium	1114:1120	arg1	ascorbate					1122:1130	calcium ascorbate	1114:1130	calcium ascorbate	1114:1130	Thus, the presence of calcium ascorbate influenced the properties of A3 film.					
30031085	3	26	theme	chemical	536:543	arg1	routes					555:560	two chemical reduction routes	532:560	two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4	532:631	Colloidal AgNPs were synthetized by two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4.					
30031085	6	27	theme	films	978:982	arg1	characterization					958:973	The characterization	954:973	The characterization of films by SEM and EDS	954:997	The characterization of films by SEM and EDS showed agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3.					
30031085	0	28	theme	Agarose-silver	0:13	arg1	films					25:29	Agarose-silver particles films	0:29	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.	0:106	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.					
30031085	3	29	theme	reduction	545:553	arg1	routes					555:560	two chemical reduction routes	532:560	two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4	532:631	Colloidal AgNPs were synthetized by two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4.					
30031085	2	30	theme	agarose	372:378	arg1	films					380:384	agarose films	372:384	agarose films	372:384	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	8	31	theme	antimicrobial	1187:1199	arg1	analysis					1201:1208	the antimicrobial analysis	1183:1208	the antimicrobial analysis	1183:1208	In addition, the antimicrobial analysis showed a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus.					
30031085	2	32	from	embedded	360:367	arg1	properties					471:480	the final properties	461:480	the final properties of the films	461:493	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	2	32	from	embedded	360:367	arg1	films					380:384	agarose films	372:384	agarose films	372:384	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	0	33	dep	nanoparticles	63:75	arg1	synthesis					77:85	synthesis	77:85	synthesis	77:85	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.					
30031085	0	33	dep	nanoparticles	63:75	arg1	properties					96:105	film properties	91:105	film properties	91:105	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.					
30031085	4	34	used	used	740:743	arg2	ascorbate					726:734	AgNPs were calcium ascorbate	707:734	AgNPs were calcium ascorbate	707:734	AgNPs synthetized using NaBH4 showed sizes ranging from 5 to 18 nm while AgNPs were calcium ascorbate was used showed micrometer from 164 to 955 nm size.					
30031085	2	35	theme	ascorbate	412:420	arg1	impact					394:399	the impact	390:399	the impact of calcium ascorbate in the formation of ANPs	390:445	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	4	36	dep	showed	664:669	arg1	showed					745:750	showed	745:750	showed sizes ranging from 5 to 18 nm while AgNPs were calcium ascorbate was used showed micrometer from 164 to 955 nm size	664:785	AgNPs synthetized using NaBH4 showed sizes ranging from 5 to 18 nm while AgNPs were calcium ascorbate was used showed micrometer from 164 to 955 nm size.					
30031085	4	37	theme	were	713:716	arg1	ascorbate					726:734	AgNPs were calcium ascorbate	707:734	AgNPs were calcium ascorbate	707:734	AgNPs synthetized using NaBH4 showed sizes ranging from 5 to 18 nm while AgNPs were calcium ascorbate was used showed micrometer from 164 to 955 nm size.					
30031085	6	38	theme	A3	1088:1089	arg1	surface					1077:1083	surface	1077:1083	surface of A3	1077:1089	The characterization of films by SEM and EDS showed agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3.					
30031085	1	39	theme	New	108:110	arg1	nanoparticles					112:124	New nanoparticles	108:124	New nanoparticles containing biomaterials	108:148	New nanoparticles containing biomaterials are emerging as versatile active platforms in a great number of applications, for example, as skin substitutes and therapeutic media.					
30031085	8	40	theme	film	1262:1265	arg1	composition					1267:1277	the film composition	1258:1277	the film composition	1258:1277	In addition, the antimicrobial analysis showed a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus.					
30031085	2	41	theme	calcium	404:410	arg1	ascorbate					412:420	calcium ascorbate	404:420	calcium ascorbate	404:420	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	5	42	theme	calcium	882:888	arg1	ascorbate					890:898	calcium ascorbate	882:898	calcium ascorbate (A2)	882:903	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	5	42	theme	calcium	882:888	arg1	A2					901:902	A2	901:902	A2	901:902	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	9	43	theme	important	1385:1393	arg1	way					1395:1397	an important way	1382:1397	an important way for development of new biomaterials, economically competitive, and with medical application	1382:1489	The results found in this study open an important way for development of new biomaterials, economically competitive, and with medical application.					
30031085	5	44	theme	calcium	930:936	arg1	ascorbate					938:946	calcium ascorbate	930:946	calcium ascorbate (A3)	930:951	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	5	44	theme	calcium	930:936	arg1	A3					949:950	A3	949:950	A3	949:950	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	1	45	theme	great	198:202	arg1	number					204:209	a great number	196:209	a great number of applications	196:225	New nanoparticles containing biomaterials are emerging as versatile active platforms in a great number of applications, for example, as skin substitutes and therapeutic media.					
30031085	1	46	theme	applications	214:225	arg1	number					204:209	a great number	196:209	a great number of applications	196:225	New nanoparticles containing biomaterials are emerging as versatile active platforms in a great number of applications, for example, as skin substitutes and therapeutic media.					
30031085	2	47	from	properties	471:480	arg1	embedded					360:367	embedded	360:367	embedded	360:367	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	2	48	from	films	380:384	arg1	embedded					360:367	embedded	360:367	embedded	360:367	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	0	49	theme	ascorbate	50:58	arg1	Effect					32:37	Effect	32:37	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.	0:106	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.					
30031085	8	50	theme	release	1236:1242	arg1	dependence					1244:1253	a silver particles release dependence	1217:1253	a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus	1217:1342	In addition, the antimicrobial analysis showed a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus.					
30031085	7	51	theme	ascorbate	1122:1130	arg1	presence					1102:1109	the presence	1098:1109	the presence of calcium ascorbate	1098:1130	Thus, the presence of calcium ascorbate influenced the properties of A3 film.					
30031085	8	52	theme	presented	1295:1303	arg1	activity					1305:1312	only the A3 presented activity	1283:1312	only the A3 presented activity against Staphylococcus aureus	1283:1342	In addition, the antimicrobial analysis showed a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus.					
30031085	6	53	from	agglomerates	1014:1025	arg1	surface					1077:1083	surface	1077:1083	surface of A3	1077:1089	The characterization of films by SEM and EDS showed agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3.					
30031085	6	53	from	agglomerates	1014:1025	arg1	crystals					1065:1072	unreacted calcium ascorbate crystals	1037:1072	unreacted calcium ascorbate crystals	1037:1072	The characterization of films by SEM and EDS showed agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3.					
30031085	6	53	from	agglomerates	1014:1025	arg1	A2					1030:1031	A2	1030:1031	A2	1030:1031	The characterization of films by SEM and EDS showed agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3.					
30031085	5	54	theme	control	839:845	arg1	film					847:850	agarose control film	831:850	three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3)	811:951	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	5	54	theme	control	839:845	arg1	A1					853:854	A1	853:854	A1	853:854	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	2	55	theme	ANPs	442:445	arg1	formation					429:437	the formation	425:437	the formation of ANPs	425:445	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	9	56	theme	biomaterials	1422:1433	arg1	development					1403:1413	development	1403:1413	development of new biomaterials, economically competitive,	1403:1460	The results found in this study open an important way for development of new biomaterials, economically competitive, and with medical application.					
30031085	2	57	theme	nanoparticles	338:350	arg1	preparation					316:326	the preparation	312:326	the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films	312:493	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	4	58	dep	18 nm	695:699	arg1	to					692:693	to	692:693	to	692:693	AgNPs synthetized using NaBH4 showed sizes ranging from 5 to 18 nm while AgNPs were calcium ascorbate was used showed micrometer from 164 to 955 nm size.					
30031085	3	59	theme	calcium	576:582	arg1	ascorbate					584:592	calcium ascorbate	576:592	calcium ascorbate	576:592	Colloidal AgNPs were synthetized by two chemical reduction routes: (i) applying calcium ascorbate and NaBH4 and (ii) applying only NaBH4.					
30031085	2	60	theme	silver	331:336	arg1	nanoparticles					338:350	silver nanoparticles	331:350	silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films	331:493	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	2	60	theme	silver	331:336	arg1	AgNPs					353:357	AgNPs	353:357	AgNPs	353:357	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	6	61	theme	calcium	1047:1053	arg1	crystals					1065:1072	unreacted calcium ascorbate crystals	1037:1072	unreacted calcium ascorbate crystals	1037:1072	The characterization of films by SEM and EDS showed agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3.					
30031085	1	62	dep	skin	244:247	arg1	substitutes					249:259	substitutes	249:259	substitutes	249:259	New nanoparticles containing biomaterials are emerging as versatile active platforms in a great number of applications, for example, as skin substitutes and therapeutic media.					
30031085	0	63	dep	films	25:29	arg1	Effect					32:37	Effect	32:37	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.	0:106	Agarose-silver particles films: Effect of calcium ascorbate in nanoparticles synthesis and film properties.					
30031085	7	64	theme	film	1164:1167	arg1	properties					1147:1156	the properties	1143:1156	the properties of A3 film	1143:1167	Thus, the presence of calcium ascorbate influenced the properties of A3 film.					
30031085	8	65	theme	silver	1219:1224	arg1	dependence					1244:1253	a silver particles release dependence	1217:1253	a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus	1217:1342	In addition, the antimicrobial analysis showed a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus.					
30031085	7	66	theme	A3	1161:1162	arg1	film					1164:1167	A3 film	1161:1167	A3 film	1161:1167	Thus, the presence of calcium ascorbate influenced the properties of A3 film.					
30031085	5	67	with	agarose + AgNPs	909:923	arg1	ascorbate					938:946	calcium ascorbate	930:946	calcium ascorbate (A3)	930:951	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	5	67	with	agarose + AgNPs	909:923	arg1	A3					949:950	A3	949:950	A3	949:950	Films were prepared in three formulations: agarose control film (A1); agarose + AgNPs without calcium ascorbate (A2) and agarose + AgNPs with calcium ascorbate (A3).					
30031085	2	68	from	impact	394:399	arg1	formation					429:437	the formation	425:437	the formation of ANPs	425:445	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	6	69	theme	agarose	1006:1012	arg1	agglomerates					1014:1025	agarose agglomerates	1006:1025	agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3	1006:1089	The characterization of films by SEM and EDS showed agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3.					
30031085	8	70	from	dependence	1244:1253	arg1	composition					1267:1277	the film composition	1258:1277	the film composition	1258:1277	In addition, the antimicrobial analysis showed a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus.					
30031085	8	70	from	dependence	1244:1253	arg1	activity					1305:1312	only the A3 presented activity	1283:1312	only the A3 presented activity against Staphylococcus aureus	1283:1342	In addition, the antimicrobial analysis showed a silver particles release dependence on the film composition and only the A3 presented activity against Staphylococcus aureus.					
30031085	2	71	theme	final	465:469	arg1	properties					471:480	the final properties	461:480	the final properties of the films	461:493	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	6	72	theme	unreacted	1037:1045	arg1	crystals					1065:1072	unreacted calcium ascorbate crystals	1037:1072	unreacted calcium ascorbate crystals	1037:1072	The characterization of films by SEM and EDS showed agarose agglomerates in A2 and unreacted calcium ascorbate crystals on surface of A3.					
30031085	9	73	theme	medical	1471:1477	arg1	application					1479:1489	medical application	1471:1489	medical application	1471:1489	The results found in this study open an important way for development of new biomaterials, economically competitive, and with medical application.					
30031085	9	74	theme	competitive	1449:1459	arg1	biomaterials					1422:1433	new biomaterials	1418:1433	new biomaterials	1418:1433	The results found in this study open an important way for development of new biomaterials, economically competitive, and with medical application.					
30031085	2	75	theme	embedded	360:367	arg1	nanoparticles					338:350	silver nanoparticles	331:350	silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films	331:493	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	2	75	theme	embedded	360:367	arg1	AgNPs					353:357	AgNPs	353:357	AgNPs	353:357	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
30031085	2	76	theme	films	489:493	arg1	properties					471:480	the final properties	461:480	the final properties of the films	461:493	The present study describes the preparation of silver nanoparticles (AgNPs) embedded in agarose films and the impact of calcium ascorbate in the formation of ANPs as well as in the final properties of the films.					
31627459	5	0	theme	combustion	744:753	arg1	characteristics					755:769	combustion characteristics	744:769	combustion characteristics	744:769	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	1	1	theme	UV-induced	304:313	arg1	process					345:351	UV-induced photo grafting polymerization process	304:351	UV-induced photo grafting polymerization process	304:351	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	3	2	theme	flame-retardant	471:485	arg1	FR-PAN					499:504	FR-PAN	499:504	FR-PAN	499:504	Finally, the flame-retardant PAN fabric (FR-PAN) was prepared by phosphorylation.					
31627459	3	2	theme	flame-retardant	471:485	arg1	fabric					491:496	the flame-retardant PAN fabric	467:496	the flame-retardant PAN fabric (FR-PAN)	467:505	Finally, the flame-retardant PAN fabric (FR-PAN) was prepared by phosphorylation.					
31627459	8	3	theme	burned	1257:1262	arg1	fabric					1271:1276	the burned FR-PAN fabric	1253:1276	the burned FR-PAN fabric	1253:1276	In addition, the char residue of the burned FR-PAN fabric is over 97%, indicating excellent char-forming ability.					
31627459	1	4	theme	photo	315:319	arg1	process					345:351	UV-induced photo grafting polymerization process	304:351	UV-induced photo grafting polymerization process	304:351	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	3	5	theme	PAN	487:489	arg1	FR-PAN					499:504	FR-PAN	499:504	FR-PAN	499:504	Finally, the flame-retardant PAN fabric (FR-PAN) was prepared by phosphorylation.					
31627459	3	5	theme	PAN	487:489	arg1	fabric					491:496	the flame-retardant PAN fabric	467:496	the flame-retardant PAN fabric (FR-PAN)	467:505	Finally, the flame-retardant PAN fabric (FR-PAN) was prepared by phosphorylation.					
31627459	0	6	theme	Fabric	118:123	arg1	Retardancy					86:95	Flame Retardancy	80:95	Flame Retardancy of Polyacrylonitrile Fabric	80:123	Fabrication of Chitosan-Based Intumescent Flame Retardant Coating for Improving Flame Retardancy of Polyacrylonitrile Fabric.					
31627459	1	7	theme	grafting	321:328	arg1	process					345:351	UV-induced photo grafting polymerization process	304:351	UV-induced photo grafting polymerization process	304:351	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	8	8	theme	char-forming	1312:1323	arg1	ability					1325:1331	excellent char-forming ability	1302:1331	excellent char-forming ability	1302:1331	In addition, the char residue of the burned FR-PAN fabric is over 97%, indicating excellent char-forming ability.					
31627459	6	9	theme	decomposition	944:956	arg1	temperature					958:968	the onset thermal decomposition temperature	926:968	the onset thermal decomposition temperature of FR-PAN fabric	926:985	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	6	9	theme	decomposition	944:956	arg1	lower					990:994	lower	990:994	lower	990:994	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	0	10	theme	Polyacrylonitrile	100:116	arg1	Fabric					118:123	Polyacrylonitrile Fabric	100:123	Polyacrylonitrile Fabric	100:123	Fabrication of Chitosan-Based Intumescent Flame Retardant Coating for Improving Flame Retardancy of Polyacrylonitrile Fabric.					
31627459	1	11	theme	polymerization	330:343	arg1	process					345:351	UV-induced photo grafting polymerization process	304:351	UV-induced photo grafting polymerization process	304:351	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	6	12	theme	thermal	936:942	arg1	temperature					958:968	the onset thermal decomposition temperature	926:968	the onset thermal decomposition temperature of FR-PAN fabric	926:985	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	6	12	theme	thermal	936:942	arg1	lower					990:994	lower	990:994	lower	990:994	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	4	13	theme	photoelectron	671:683	arg1	XPS					699:701	XPS	699:701	XPS	699:701	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
31627459	4	13	theme	photoelectron	671:683	arg1	spectroscopy					685:696	X-ray photoelectron spectroscopy	665:696	X-ray photoelectron spectroscopy (XPS)	665:702	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
31627459	4	14	theme	elemental	558:566	arg1	analysis					568:575	elemental analysis	558:575	elemental analysis	558:575	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
31627459	4	15	theme	X-ray	665:669	arg1	XPS					699:701	XPS	699:701	XPS	699:701	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
31627459	4	15	theme	X-ray	665:669	arg1	spectroscopy					685:696	X-ray photoelectron spectroscopy	665:696	X-ray photoelectron spectroscopy (XPS)	665:702	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
31627459	5	16	theme	differential	836:847	arg1	DSC					871:873	DSC	871:873	DSC	871:873	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	5	16	theme	differential	836:847	arg1	calorimetry					858:868	differential scanning calorimetry	836:868	differential scanning calorimetry (DSC)	836:874	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	5	17	theme	degradation	717:727	arg1	properties					729:738	The thermal degradation properties	705:738	The thermal degradation properties	705:738	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	1	18	theme	PAN	189:191	arg1	fabrics					194:200	polyacrylonitrile (PAN) fabrics	170:200	polyacrylonitrile (PAN) fabrics	170:200	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	2	19	theme	PAN	429:431	arg1	PAN-g-GMA-g-CS					441:454	PAN-g-GMA-g-CS	441:454	PAN-g-GMA-g-CS	441:454	Then, PAN-g-GMA was chemically grafted with chitosan to obtain a bigrafted PAN fabric (PAN-g-GMA-g-CS).					
31627459	2	19	theme	PAN	429:431	arg1	fabric					433:438	a bigrafted PAN fabric	417:438	a bigrafted PAN fabric (PAN-g-GMA-g-CS)	417:455	Then, PAN-g-GMA was chemically grafted with chitosan to obtain a bigrafted PAN fabric (PAN-g-GMA-g-CS).					
31627459	7	20	theme	combustion	1178:1187	arg1	rate					1189:1192	the combustion rate	1174:1192	the combustion rate	1174:1192	The combustion test indicates that the FR-PAN fabric has an excellent flame-retardant property and the combustion rate is significantly reduced.					
31627459	8	21	theme	excellent	1302:1310	arg1	ability					1325:1331	excellent char-forming ability	1302:1331	excellent char-forming ability	1302:1331	In addition, the char residue of the burned FR-PAN fabric is over 97%, indicating excellent char-forming ability.					
31627459	2	22	theme	bigrafted	419:427	arg1	PAN-g-GMA-g-CS					441:454	PAN-g-GMA-g-CS	441:454	PAN-g-GMA-g-CS	441:454	Then, PAN-g-GMA was chemically grafted with chitosan to obtain a bigrafted PAN fabric (PAN-g-GMA-g-CS).					
31627459	2	22	theme	bigrafted	419:427	arg1	fabric					433:438	a bigrafted PAN fabric	417:438	a bigrafted PAN fabric (PAN-g-GMA-g-CS)	417:455	Then, PAN-g-GMA was chemically grafted with chitosan to obtain a bigrafted PAN fabric (PAN-g-GMA-g-CS).					
31627459	6	23	theme	onset	930:934	arg1	temperature					958:968	the onset thermal decomposition temperature	926:968	the onset thermal decomposition temperature of FR-PAN fabric	926:985	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	6	23	theme	onset	930:934	arg1	lower					990:994	lower	990:994	lower	990:994	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	7	24	contain	has	1128:1130	arg2	property					1161:1168	an excellent flame-retardant property	1132:1168	an excellent flame-retardant property	1132:1168	The combustion test indicates that the FR-PAN fabric has an excellent flame-retardant property and the combustion rate is significantly reduced.					
31627459	7	24	contain	has	1128:1130	arg1	fabric					1121:1126	the FR-PAN fabric	1110:1126	the FR-PAN fabric	1110:1126	The combustion test indicates that the FR-PAN fabric has an excellent flame-retardant property and the combustion rate is significantly reduced.					
31627459	1	25	theme	fabrics	194:200	arg1	retardancy					156:165	the flame retardancy	146:165	the flame retardancy of polyacrylonitrile (PAN) fabrics	146:200	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	0	26	theme	Intumescent	30:40	arg1	Coating					58:64	Chitosan-Based Intumescent Flame Retardant Coating	15:64	Chitosan-Based Intumescent Flame Retardant Coating	15:64	Fabrication of Chitosan-Based Intumescent Flame Retardant Coating for Improving Flame Retardancy of Polyacrylonitrile Fabric.					
31627459	1	27	theme	PAN	269:271	arg1	fabric					273:278	PAN fabric	269:278	PAN fabric (PAN-g-GMA)	269:290	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	1	27	theme	PAN	269:271	arg1	PAN-g-GMA					281:289	PAN-g-GMA	281:289	PAN-g-GMA	281:289	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	8	28	theme	fabric	1271:1276	arg1	fabric					1271:1276	the burned FR-PAN fabric	1253:1276	the burned FR-PAN fabric	1253:1276	In addition, the char residue of the burned FR-PAN fabric is over 97%, indicating excellent char-forming ability.					
31627459	8	28	theme	fabric	1271:1276	arg1	residue					1242:1248	the char residue	1233:1248	the char residue of the burned FR-PAN fabric	1233:1276	In addition, the char residue of the burned FR-PAN fabric is over 97%, indicating excellent char-forming ability.					
31627459	6	29	theme	due	1028:1030	arg1	sample					1021:1026	the control sample	1009:1026	the control sample due to the degradation of the grafting groups	1009:1072	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	0	30	theme	Chitosan-Based	15:28	arg1	Coating					58:64	Chitosan-Based Intumescent Flame Retardant Coating	15:64	Chitosan-Based Intumescent Flame Retardant Coating	15:64	Fabrication of Chitosan-Based Intumescent Flame Retardant Coating for Improving Flame Retardancy of Polyacrylonitrile Fabric.					
31627459	1	31	theme	fabric	273:278	arg1	surface					258:264	the surface	254:264	the surface of PAN fabric (PAN-g-GMA)	254:290	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	1	32	theme	polyacrylonitrile	170:186	arg1	fabrics					194:200	polyacrylonitrile (PAN) fabrics	170:200	polyacrylonitrile (PAN) fabrics	170:200	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	1	33	theme	glycidyl	203:210	arg1	GMA					226:228	GMA	226:228	GMA	226:228	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	1	33	theme	glycidyl	203:210	arg1	methacrylate					212:223	glycidyl methacrylate	203:223	glycidyl methacrylate (GMA)	203:229	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	0	34	theme	Retardant	48:56	arg1	Coating					58:64	Chitosan-Based Intumescent Flame Retardant Coating	15:64	Chitosan-Based Intumescent Flame Retardant Coating	15:64	Fabrication of Chitosan-Based Intumescent Flame Retardant Coating for Improving Flame Retardancy of Polyacrylonitrile Fabric.					
31627459	4	35	dep	transform	622:630	arg1	infrared					632:639	infrared	632:639	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)	622:702	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
31627459	6	36	theme	control	1013:1019	arg1	sample					1021:1026	the control sample	1009:1026	the control sample due to the degradation of the grafting groups	1009:1072	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	0	37	theme	Flame	42:46	arg1	Coating					58:64	Chitosan-Based Intumescent Flame Retardant Coating	15:64	Chitosan-Based Intumescent Flame Retardant Coating	15:64	Fabrication of Chitosan-Based Intumescent Flame Retardant Coating for Improving Flame Retardancy of Polyacrylonitrile Fabric.					
31627459	4	38	dep	Fourier	614:620	arg1	transform					622:630	transform	622:630	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS)	622:702	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
31627459	5	39	theme	thermogravimetric	803:819	arg1	TG					831:832	TG	831:832	TG	831:832	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	5	39	theme	thermogravimetric	803:819	arg1	analysis					821:828	thermogravimetric analysis	803:828	thermogravimetric analysis (TG)	803:833	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	5	40	theme	fabrics	778:784	arg1	properties					729:738	The thermal degradation properties	705:738	The thermal degradation properties	705:738	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	5	40	theme	fabrics	778:784	arg1	characteristics					755:769	combustion characteristics	744:769	combustion characteristics	744:769	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	7	41	theme	flame-retardant	1145:1159	arg1	property					1161:1168	an excellent flame-retardant property	1132:1168	an excellent flame-retardant property	1132:1168	The combustion test indicates that the FR-PAN fabric has an excellent flame-retardant property and the combustion rate is significantly reduced.					
31627459	4	42	dep	structure	544:552	arg1	The					540:542	The	540:542	The	540:542	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
31627459	5	43	theme	cone	881:884	arg1	calorimeter					886:896	cone calorimeter	881:896	cone calorimeter (CC)	881:901	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	5	43	theme	cone	881:884	arg1	CC					899:900	CC	899:900	CC	899:900	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	0	44	theme	Coating	58:64	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of Chitosan-Based Intumescent Flame Retardant Coating for Improving Flame Retardancy of Polyacrylonitrile Fabric.	0:124	Fabrication of Chitosan-Based Intumescent Flame Retardant Coating for Improving Flame Retardancy of Polyacrylonitrile Fabric.					
31627459	7	45	theme	excellent	1135:1143	arg1	property					1161:1168	an excellent flame-retardant property	1132:1168	an excellent flame-retardant property	1132:1168	The combustion test indicates that the FR-PAN fabric has an excellent flame-retardant property and the combustion rate is significantly reduced.					
31627459	8	46	theme	char	1237:1240	arg1	fabric					1271:1276	the burned FR-PAN fabric	1253:1276	the burned FR-PAN fabric	1253:1276	In addition, the char residue of the burned FR-PAN fabric is over 97%, indicating excellent char-forming ability.					
31627459	8	46	theme	char	1237:1240	arg1	residue					1242:1248	the char residue	1233:1248	the char residue of the burned FR-PAN fabric	1233:1276	In addition, the char residue of the burned FR-PAN fabric is over 97%, indicating excellent char-forming ability.					
31627459	6	47	theme	groups	1067:1072	arg1	degradation					1039:1049	the degradation	1035:1049	the degradation of the grafting groups	1035:1072	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	8	48	from	%	1288:1288	arg1	addition					1223:1230	addition	1223:1230	addition	1223:1230	In addition, the char residue of the burned FR-PAN fabric is over 97%, indicating excellent char-forming ability.					
31627459	5	49	theme	thermal	709:715	arg1	properties					729:738	The thermal degradation properties	705:738	The thermal degradation properties	705:738	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	8	50	theme	FR-PAN	1264:1269	arg1	fabric					1271:1276	the burned FR-PAN fabric	1253:1276	the burned FR-PAN fabric	1253:1276	In addition, the char residue of the burned FR-PAN fabric is over 97%, indicating excellent char-forming ability.					
31627459	6	51	theme	grafting	1058:1065	arg1	groups					1067:1072	the grafting groups	1054:1072	the grafting groups	1054:1072	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	0	52	theme	Flame	80:84	arg1	Retardancy					86:95	Flame Retardancy	80:95	Flame Retardancy of Polyacrylonitrile Fabric	80:123	Fabrication of Chitosan-Based Intumescent Flame Retardant Coating for Improving Flame Retardancy of Polyacrylonitrile Fabric.					
31627459	1	53	theme	flame	150:154	arg1	retardancy					156:165	the flame retardancy	146:165	the flame retardancy of polyacrylonitrile (PAN) fabrics	146:200	In order to improve the flame retardancy of polyacrylonitrile (PAN) fabrics, glycidyl methacrylate (GMA) was first grafted onto the surface of PAN fabric (PAN-g-GMA) by means of UV-induced photo grafting polymerization process.					
31627459	5	54	theme	scanning	849:856	arg1	DSC					871:873	DSC	871:873	DSC	871:873	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	5	54	theme	scanning	849:856	arg1	calorimetry					858:868	differential scanning calorimetry	836:868	differential scanning calorimetry (DSC)	836:874	The thermal degradation properties and combustion characteristics of the fabrics were accessed by thermogravimetric analysis (TG), differential scanning calorimetry (DSC), and cone calorimeter (CC).					
31627459	6	55	theme	fabric	980:985	arg1	temperature					958:968	the onset thermal decomposition temperature	926:968	the onset thermal decomposition temperature of FR-PAN fabric	926:985	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	6	55	theme	fabric	980:985	arg1	lower					990:994	lower	990:994	lower	990:994	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	7	56	theme	combustion	1079:1088	arg1	test					1090:1093	The combustion test	1075:1093	The combustion test	1075:1093	The combustion test indicates that the FR-PAN fabric has an excellent flame-retardant property and the combustion rate is significantly reduced.					
31627459	6	57	theme	FR-PAN	973:978	arg1	fabric					980:985	FR-PAN fabric	973:985	FR-PAN fabric	973:985	The results show that the onset thermal decomposition temperature of FR-PAN fabric is lower than that of the control sample due to the degradation of the grafting groups.					
31627459	7	58	theme	FR-PAN	1114:1119	arg1	fabric					1121:1126	the FR-PAN fabric	1110:1126	the FR-PAN fabric	1110:1126	The combustion test indicates that the FR-PAN fabric has an excellent flame-retardant property and the combustion rate is significantly reduced.					
31627459	4	59	theme	samples	584:590	arg1	structure					544:552	structure	544:552	structure	544:552	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
31627459	4	59	theme	samples	584:590	arg1	analysis					568:575	elemental analysis	558:575	elemental analysis	558:575	The structure and elemental analysis of the samples were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS).					
30826905	8	0	from	dysbiosis	1059:1067	arg1	brain					1247:1251	brain	1247:1251	brain	1247:1251	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	0	from	dysbiosis	1059:1067	arg1	colon					1237:1241	colon	1237:1241	colon	1237:1241	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	10	1	theme	beneficial	1690:1699	arg1	effects					1701:1707	the beneficial effects	1686:1707	the beneficial effects of psychobiotics in neuroinflammation and behavioral performance	1686:1772	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	3	2	theme	knowledge	458:466	arg1	gap					468:470	a wide knowledge gap	451:470	a wide knowledge gap	451:470	However, a wide knowledge gap exists about the gut microbial effects on the function of glia cells.					
30826905	3	3	theme	microbial	493:501	arg1	effects					503:509	the gut microbial effects	485:509	the gut microbial effects on the function of glia cells	485:539	However, a wide knowledge gap exists about the gut microbial effects on the function of glia cells.					
30826905	8	4	theme	neurotrophic	1112:1123	arg1	factor					1125:1130	glial cell-derived neurotrophic factor	1093:1130	glial cell-derived neurotrophic factor (GDNF)	1093:1137	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	4	theme	neurotrophic	1112:1123	arg1	GDNF					1133:1136	GDNF	1133:1136	GDNF	1133:1136	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	10	5	theme	gut	1782:1784	arg1	changes					1797:1803	gut microbiota changes	1782:1803	gut microbiota changes	1782:1803	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	6	theme	such	1576:1579	arg1	changes					1581:1587	such changes	1576:1587	such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis	1576:1863	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	6	7	theme	Fecal	869:873	arg1	sample					875:880	Fecal sample	869:880	Fecal sample	869:880	Fecal sample was collected to evaluate gut microbial composition.					
30826905	4	8	theme	enteric	656:662	arg1	system					684:689	enteric and central nervous system	656:689	system	684:689	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	8	9	theme	fibrillary	1146:1155	arg1	protein					1164:1170	glial fibrillary acidic protein	1140:1170	glial fibrillary acidic protein (GFAP)	1140:1177	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	9	theme	fibrillary	1146:1155	arg1	GFAP					1173:1176	GFAP	1173:1176	GFAP	1173:1176	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	10	theme	glial	1140:1144	arg1	protein					1164:1170	glial fibrillary acidic protein	1140:1170	glial fibrillary acidic protein (GFAP)	1140:1177	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	10	theme	glial	1140:1144	arg1	GFAP					1173:1176	GFAP	1173:1176	GFAP	1173:1176	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	10	11	theme	plantarum	1448:1456	arg1	administration					1427:1440	the administration	1423:1440	the administration of L. plantarum	1423:1456	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	8	12	theme	glial	1093:1097	arg1	factor					1125:1130	glial cell-derived neurotrophic factor	1093:1130	glial cell-derived neurotrophic factor (GDNF)	1093:1137	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	12	theme	glial	1093:1097	arg1	GDNF					1133:1136	GDNF	1133:1136	GDNF	1133:1136	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	13	theme	acidic	1157:1162	arg1	protein					1164:1170	glial fibrillary acidic protein	1140:1170	glial fibrillary acidic protein (GFAP)	1140:1177	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	13	theme	acidic	1157:1162	arg1	GFAP					1173:1176	GFAP	1173:1176	GFAP	1173:1176	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	0	14	theme	brain	140:144	arg1	regions					146:152	brain regions	140:152	brain regions	140:152	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	3	15	theme	glia	530:533	arg1	cells					535:539	glia cells	530:539	glia cells	530:539	However, a wide knowledge gap exists about the gut microbial effects on the function of glia cells.					
30826905	8	16	dep	colon	1237:1241	arg1	the					1233:1235	the	1233:1235	the	1233:1235	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	0	17	from	rats	93:96	arg1	effects					56:62	anti-inflammatory and anxiolytic effects	23:62	anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats	23:96	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	10	18	theme	possible	1818:1825	arg1	role					1827:1830	possible role	1818:1830	possible role of glial cells in gut-brain axis	1818:1863	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	9	19	theme	concurrent	1263:1272	arg1	supplementation					1274:1288	concurrent supplementation	1263:1288	concurrent supplementation of L. plantarum and inulin	1263:1315	However, concurrent supplementation of L. plantarum and inulin could improve the gut microbial composition as well as reduce the levels of inflammatory cytokines.					
30826905	9	20	theme	microbial	1339:1347	arg1	composition					1349:1359	the gut microbial composition	1331:1359	the gut microbial composition	1331:1359	However, concurrent supplementation of L. plantarum and inulin could improve the gut microbial composition as well as reduce the levels of inflammatory cytokines.					
30826905	6	21	theme	gut	908:910	arg1	composition					922:932	gut microbial composition	908:932	gut microbial composition	908:932	Fecal sample was collected to evaluate gut microbial composition.					
30826905	10	22	theme	gut-brain	1850:1858	arg1	axis					1860:1863	gut-brain axis	1850:1863	gut-brain axis	1850:1863	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	2	23	from	role	299:302	arg1	management					333:342	the management	329:342	the management of many diseases including T2DM	329:374	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	8	24	theme	markers	1197:1203	arg1	dysbiosis					1059:1067	dysbiosis	1059:1067	dysbiosis	1059:1067	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	24	theme	markers	1197:1203	arg1	levels					1083:1088	increased levels	1073:1088	increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain	1073:1251	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	10	25	theme	cells	1841:1845	arg1	role					1827:1830	possible role	1818:1830	possible role of glial cells in gut-brain axis	1818:1863	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	26	theme	synbiotic	1548:1556	arg1	intake					1558:1563	the synbiotic intake	1544:1563	the synbiotic intake	1544:1563	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	4	27	dep	dysbiosis	619:627	arg1	function					644:651	function	644:651	function	644:651	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	4	28	theme	inflammatory	699:710	arg1	insight					712:718	an inflammatory insight	696:718	an inflammatory insight in T2DM	696:726	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	0	29	theme	possible	99:106	arg1	link					108:111	possible link	99:111	possible link between gut microbiota and brain regions	99:152	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	2	30	theme	activity	412:419	arg1	role					299:302	The role	295:302	The role of the gut-brain axis in the management of many diseases including T2DM	295:374	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	2	30	theme	activity	412:419	arg1	focus					389:393	the focus	385:393	the focus of much research activity in the recent years	385:439	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	2	31	theme	diseases	352:359	arg1	management					333:342	the management	329:342	the management of many diseases including T2DM	329:374	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	2	32	theme	much	398:401	arg1	activity					412:419	much research activity	398:419	much research activity	398:419	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	4	33	theme	nervous	676:682	arg1	system					684:689	enteric and central nervous system	656:689	system	684:689	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	9	34	theme	cytokines	1406:1414	arg1	levels					1383:1388	the levels	1379:1388	the levels of inflammatory cytokines	1379:1414	However, concurrent supplementation of L. plantarum and inulin could improve the gut microbial composition as well as reduce the levels of inflammatory cytokines.					
30826905	1	35	theme	Type	163:166	arg1	T2DM					189:192	T2DM	189:192	T2DM	189:192	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	1	35	theme	Type	163:166	arg1	mellitus					179:186	Type 2 diabetes mellitus	163:186	PURPOSE Type 2 diabetes mellitus (T2DM)	155:193	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	0	36	theme	anti-inflammatory	23:39	arg1	effects					56:62	anti-inflammatory and anxiolytic effects	23:62	anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats	23:96	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	2	37	theme	axis	321:324	arg1	focus					389:393	the focus	385:393	the focus of much research activity in the recent years	385:439	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	2	37	theme	axis	321:324	arg1	role					299:302	The role	295:302	The role of the gut-brain axis in the management of many diseases including T2DM	295:374	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	0	38	theme	anxiolytic	45:54	arg1	effects					56:62	anti-inflammatory and anxiolytic effects	23:62	anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats	23:96	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	10	39	from	decrease	1479:1486	arg1	TLR-2					1491:1495	TLR-2	1491:1495	TLR-2	1491:1495	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	39	from	decrease	1479:1486	arg1	amygdala					1534:1541	the amygdala	1530:1541	the amygdala	1530:1541	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	1	40	theme	gut	215:217	arg1	dysbiosis					241:249	dysbiosis	241:249	dysbiosis	241:249	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	1	40	theme	gut	215:217	arg1	impairment					229:238	gut microbial impairment	215:238	gut microbial impairment (dysbiosis)	215:250	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	2	41	from	focus	389:393	arg1	years					435:439	the recent years	424:439	the recent years	424:439	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	7	42	theme	prefrontal	996:1005	arg1	PFC					1015:1017	PFC	1015:1017	PFC	1015:1017	Then, the rats were sacrificed, and the colon, amygdala, and prefrontal cortex (PFC) were studied.					
30826905	7	42	theme	prefrontal	996:1005	arg1	cortex					1007:1012	prefrontal cortex	996:1012	prefrontal cortex (PFC)	996:1018	Then, the rats were sacrificed, and the colon, amygdala, and prefrontal cortex (PFC) were studied.					
30826905	3	43	theme	wide	453:456	arg1	gap					468:470	a wide knowledge gap	451:470	a wide knowledge gap	451:470	However, a wide knowledge gap exists about the gut microbial effects on the function of glia cells.					
30826905	10	44	from	GDNF	1508:1511	arg1	TLR-2					1491:1495	TLR-2	1491:1495	TLR-2	1491:1495	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	44	from	GDNF	1508:1511	arg1	amygdala					1534:1541	the amygdala	1530:1541	the amygdala	1530:1541	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	3	45	theme	gut	489:491	arg1	effects					503:509	the gut microbial effects	485:509	the gut microbial effects on the function of glia cells	485:539	However, a wide knowledge gap exists about the gut microbial effects on the function of glia cells.					
30826905	10	46	from	GFAP	1517:1520	arg1	TLR-2					1491:1495	TLR-2	1491:1495	TLR-2	1491:1495	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	46	from	GFAP	1517:1520	arg1	amygdala					1534:1541	the amygdala	1530:1541	the amygdala	1530:1541	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	4	47	from	effects	592:598	arg1	dysbiosis					619:627	dysbiosis	619:627	dysbiosis	619:627	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	4	47	from	effects	592:598	arg1	cells					638:642	glia cells	633:642	glia cells	633:642	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	5	48	theme	L.	780:781	arg1	plantarum					784:792	Lactobacillus (L.) plantarum	765:792	Lactobacillus (L.) plantarum	765:792	METHODS Thirty rats were treated by Lactobacillus (L.) plantarum, inulin, or their combination (synbiotic) for 8 weeks after inducing T2DM.					
30826905	8	49	theme	RESULTS	1034:1040	arg1	T2DM					1042:1045	RESULTS T2DM	1034:1045	RESULTS T2DM	1034:1045	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	50	from	levels	1083:1088	arg1	brain					1247:1251	brain	1247:1251	brain	1247:1251	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	50	from	levels	1083:1088	arg1	colon					1237:1241	colon	1237:1241	colon	1237:1241	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	51	theme	cell-derived	1099:1110	arg1	factor					1125:1130	glial cell-derived neurotrophic factor	1093:1130	glial cell-derived neurotrophic factor (GDNF)	1093:1137	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	51	theme	cell-derived	1099:1110	arg1	GDNF					1133:1136	GDNF	1133:1136	GDNF	1133:1136	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	10	52	theme	psychobiotics	1712:1724	arg1	effects					1701:1707	the beneficial effects	1686:1707	the beneficial effects of psychobiotics in neuroinflammation and behavioral performance	1686:1772	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	1	53	theme	diabetes	170:177	arg1	T2DM					189:192	T2DM	189:192	T2DM	189:192	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	1	53	theme	diabetes	170:177	arg1	mellitus					179:186	Type 2 diabetes mellitus	163:186	PURPOSE Type 2 diabetes mellitus (T2DM)	155:193	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	8	54	theme	factor	1125:1130	arg1	dysbiosis					1059:1067	dysbiosis	1059:1067	dysbiosis	1059:1067	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	54	theme	factor	1125:1130	arg1	levels					1083:1088	increased levels	1073:1088	increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain	1073:1251	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	0	55	theme	gut	121:123	arg1	microbiota					125:134	gut microbiota	121:134	gut microbiota	121:134	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	10	56	from	effects	1701:1707	arg1	neuroinflammation					1729:1745	neuroinflammation	1729:1745	neuroinflammation	1729:1745	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	56	from	effects	1701:1707	arg1	performance					1762:1772	behavioral performance	1751:1772	behavioral performance	1751:1772	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	4	57	with	system	684:689	arg1	insight					712:718	an inflammatory insight	696:718	an inflammatory insight in T2DM	696:726	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	10	58	from	changes	1581:1587	arg1	colon					1596:1600	the colon	1592:1600	the colon	1592:1600	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	58	from	changes	1581:1587	arg1	CONCLUSIONS					1622:1632	PFC. CONCLUSIONS	1617:1632	PFC. CONCLUSIONS	1617:1632	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	58	from	changes	1581:1587	arg1	amygdala					1603:1610	amygdala	1603:1610	amygdala	1603:1610	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	3	59	theme	cells	535:539	arg1	function					518:525	the function	514:525	the function of glia cells	514:539	However, a wide knowledge gap exists about the gut microbial effects on the function of glia cells.					
30826905	5	60	theme	Lactobacillus	765:777	arg1	plantarum					784:792	Lactobacillus (L.) plantarum	765:792	Lactobacillus (L.) plantarum	765:792	METHODS Thirty rats were treated by Lactobacillus (L.) plantarum, inulin, or their combination (synbiotic) for 8 weeks after inducing T2DM.					
30826905	8	61	theme	increased	1073:1081	arg1	levels					1083:1088	increased levels	1073:1088	increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain	1073:1251	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	0	62	from	psychobiotics	67:79	arg1	rats					93:96	diabetic rats	84:96	diabetic rats	84:96	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	10	63	dep	colon	1596:1600	arg1	demonstrated					1647:1658	demonstrated	1647:1658	demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis	1647:1863	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	9	64	theme	inulin	1310:1315	arg1	supplementation					1274:1288	concurrent supplementation	1263:1288	concurrent supplementation of L. plantarum and inulin	1263:1315	However, concurrent supplementation of L. plantarum and inulin could improve the gut microbial composition as well as reduce the levels of inflammatory cytokines.					
30826905	10	65	from	neuroinflammation	1729:1745	arg1	effects					1701:1707	the beneficial effects	1686:1707	the beneficial effects of psychobiotics in neuroinflammation and behavioral performance	1686:1772	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	66	from	role	1827:1830	arg1	axis					1860:1863	gut-brain axis	1850:1863	gut-brain axis	1850:1863	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	4	67	theme	glia	633:636	arg1	cells					638:642	glia cells	633:642	glia cells	633:642	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	3	68	from	effects	503:509	arg1	function					518:525	the function	514:525	the function of glia cells	514:539	However, a wide knowledge gap exists about the gut microbial effects on the function of glia cells.					
30826905	10	69	theme	microbiota	1786:1795	arg1	changes					1797:1803	gut microbiota changes	1782:1803	gut microbiota changes	1782:1803	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	70	theme	PFC.	1617:1620	arg1	CONCLUSIONS					1622:1632	PFC. CONCLUSIONS	1617:1632	PFC. CONCLUSIONS	1617:1632	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	8	71	theme	protein	1164:1170	arg1	dysbiosis					1059:1067	dysbiosis	1059:1067	dysbiosis	1059:1067	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	71	theme	protein	1164:1170	arg1	levels					1083:1088	increased levels	1073:1088	increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain	1073:1251	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	0	72	theme	psychobiotics	67:79	arg1	effects					56:62	anti-inflammatory and anxiolytic effects	23:62	anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats	23:96	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	9	73	theme	gut	1335:1337	arg1	composition					1349:1359	the gut microbial composition	1331:1359	the gut microbial composition	1331:1359	However, concurrent supplementation of L. plantarum and inulin could improve the gut microbial composition as well as reduce the levels of inflammatory cytokines.					
30826905	6	74	theme	microbial	912:920	arg1	composition					922:932	gut microbial composition	908:932	gut microbial composition	908:932	Fecal sample was collected to evaluate gut microbial composition.					
30826905	10	75	dep	changes	1797:1803	arg1	focusing					1806:1813	focusing	1806:1813	focusing on possible role of glial cells in gut-brain axis	1806:1863	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	4	76	theme	psychobatics	603:614	arg1	effects					592:598	the effects	588:598	the effects of psychobatics on dysbiosis and glia cells function	588:651	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	0	77	theme	diabetic	84:91	arg1	rats					93:96	diabetic rats	84:96	diabetic rats	84:96	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	10	78	from	performance	1762:1772	arg1	effects					1701:1707	the beneficial effects	1686:1707	the beneficial effects of psychobiotics in neuroinflammation and behavioral performance	1686:1772	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	4	79	from	insight	712:718	arg1	T2DM					723:726	T2DM	723:726	T2DM	723:726	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	0	80	dep	link	108:111	arg1	insight					10:16	The novel insight	0:16	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats	0:96	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	0	81	from	effects	56:62	arg1	rats					93:96	diabetic rats	84:96	diabetic rats	84:96	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	8	82	theme	inflammatory	1184:1195	arg1	IL-6					1213:1216	IL-6	1213:1216	IL-6	1213:1216	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	82	theme	inflammatory	1184:1195	arg1	TLR-2					1223:1227	TLR-2	1223:1227	TLR-2	1223:1227	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	82	theme	inflammatory	1184:1195	arg1	IL-17					1206:1210	IL-17	1206:1210	IL-17	1206:1210	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	82	theme	inflammatory	1184:1195	arg1	markers					1197:1203	inflammatory markers	1184:1203	inflammatory markers (IL-17, IL-6, and TLR-2)	1184:1228	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	10	83	theme	glial	1835:1839	arg1	cells					1841:1845	glial cells	1835:1845	glial cells	1835:1845	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	84	theme	significant	1467:1477	arg1	decrease					1479:1486	a significant decrease	1465:1486	a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala	1465:1541	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	5	85	theme	METHODS	729:735	arg1	rats					744:747	METHODS Thirty rats	729:747	METHODS Thirty rats	729:747	METHODS Thirty rats were treated by Lactobacillus (L.) plantarum, inulin, or their combination (synbiotic) for 8 weeks after inducing T2DM.					
30826905	10	86	theme	behavioral	1751:1760	arg1	performance					1762:1772	behavioral performance	1751:1772	behavioral performance	1751:1772	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	2	87	theme	research	403:410	arg1	activity					412:419	much research activity	398:419	much research activity	398:419	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	1	88	dep	PURPOSE	155:161	arg1	T2DM					189:192	T2DM	189:192	T2DM	189:192	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	1	88	dep	PURPOSE	155:161	arg1	mellitus					179:186	Type 2 diabetes mellitus	163:186	PURPOSE Type 2 diabetes mellitus (T2DM)	155:193	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	2	89	theme	many	347:350	arg1	T2DM					371:374	T2DM	371:374	T2DM	371:374	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	2	89	theme	many	347:350	arg1	diseases					352:359	many diseases	347:359	many diseases including T2DM	347:374	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	1	90	theme	neurological	256:267	arg1	disorders					284:292	neurological and behavioral disorders	256:292	neurological and behavioral disorders	256:292	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	9	91	theme	inflammatory	1393:1404	arg1	cytokines					1406:1414	inflammatory cytokines	1393:1414	inflammatory cytokines	1393:1414	However, concurrent supplementation of L. plantarum and inulin could improve the gut microbial composition as well as reduce the levels of inflammatory cytokines.					
30826905	4	92	theme	central	668:674	arg1	system					684:689	enteric and central nervous system	656:689	system	684:689	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	1	93	theme	behavioral	273:282	arg1	disorders					284:292	neurological and behavioral disorders	256:292	neurological and behavioral disorders	256:292	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	9	94	theme	plantarum	1296:1304	arg1	supplementation					1274:1288	concurrent supplementation	1263:1288	concurrent supplementation of L. plantarum and inulin	1263:1315	However, concurrent supplementation of L. plantarum and inulin could improve the gut microbial composition as well as reduce the levels of inflammatory cytokines.					
30826905	2	95	theme	recent	428:433	arg1	years					435:439	the recent years	424:439	the recent years	424:439	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	10	96	from	psychobiotics	1712:1724	arg1	neuroinflammation					1729:1745	neuroinflammation	1729:1745	neuroinflammation	1729:1745	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	10	96	from	psychobiotics	1712:1724	arg1	performance					1762:1772	behavioral performance	1751:1772	behavioral performance	1751:1772	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	8	97	link	cell-derived	1099:1110	arg1	factor					1125:1130	glial cell-derived neurotrophic factor	1093:1130	glial cell-derived neurotrophic factor (GDNF)	1093:1137	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	97	link	cell-derived	1099:1110	arg1	GDNF					1133:1136	GDNF	1133:1136	GDNF	1133:1136	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	2	98	theme	gut-brain	311:319	arg1	axis					321:324	the gut-brain axis	307:324	the gut-brain axis	307:324	The role of the gut-brain axis in the management of many diseases including T2DM has been the focus of much research activity in the recent years.					
30826905	8	99	dep	markers	1197:1203	arg1	IL-6					1213:1216	IL-6	1213:1216	IL-6	1213:1216	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	99	dep	markers	1197:1203	arg1	TLR-2					1223:1227	TLR-2	1223:1227	TLR-2	1223:1227	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	99	dep	markers	1197:1203	arg1	IL-17					1206:1210	IL-17	1206:1210	IL-17	1206:1210	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	8	99	dep	markers	1197:1203	arg1	markers					1197:1203	inflammatory markers	1184:1203	inflammatory markers (IL-17, IL-6, and TLR-2)	1184:1228	RESULTS T2DM resulted in dysbiosis and increased levels of glial cell-derived neurotrophic factor (GDNF), glial fibrillary acidic protein (GFAP), and inflammatory markers (IL-17, IL-6, and TLR-2) in the colon and brain.					
30826905	10	100	theme	innovative	1663:1672	arg1	approach					1674:1681	an innovative approach	1660:1681	an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance	1660:1772	While the administration of L. plantarum led to a significant decrease in TLR-2 as well as GDNF and GFAP only in the amygdala, the synbiotic intake could make such changes in the colon, amygdala, and PFC. CONCLUSIONS Our findings demonstrated an innovative approach to the beneficial effects of psychobiotics in neuroinflammation and behavioral performance through gut microbiota changes, focusing on possible role of glial cells in gut-brain axis.					
30826905	4	101	theme	present	553:559	arg1	study					561:565	the present study	549:565	the present study	549:565	Hence, the present study was aimed to examine the effects of psychobatics on dysbiosis and glia cells function in enteric and central nervous system with an inflammatory insight in T2DM.					
30826905	0	102	theme	novel	4:8	arg1	insight					10:16	The novel insight	0:16	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats	0:96	The novel insight into anti-inflammatory and anxiolytic effects of psychobiotics in diabetic rats: possible link between gut microbiota and brain regions.					
30826905	1	103	theme	microbial	219:227	arg1	dysbiosis					241:249	dysbiosis	241:249	dysbiosis	241:249	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30826905	1	103	theme	microbial	219:227	arg1	impairment					229:238	gut microbial impairment	215:238	gut microbial impairment (dysbiosis)	215:250	PURPOSE Type 2 diabetes mellitus (T2DM) was associated with gut microbial impairment (dysbiosis) and neurological and behavioral disorders.					
30982065	4	0	theme	feed	768:771	arg1	additive/kg					753:763	additive/kg	753:763	additive/kg of feed	753:771	After hatching, the chicks from both incubation treatments were submitted to additional treatments during the growth phase from 1 to 42 D of age (diet without and with 0.74 g of additive/kg of feed).					
30982065	7	1	theme	variance	976:983	arg1	analysis					985:992	variance analysis	976:992	variance analysis using the general linear model procedure of SAS (SAS Institute)	976:1056	The data were submitted to variance analysis using the general linear model procedure of SAS (SAS Institute).					
30982065	9	2	theme	additive	1446:1453	arg1	eggs					1485:1488	additive during rearing, or uninjected eggs	1446:1488	additive during rearing, or uninjected eggs	1446:1488	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	11	3	theme	chondroitin	1868:1878	arg1	glycosaminoglycans					1848:1865	glycosaminoglycans	1848:1865	glycosaminoglycans (chondroitin sulfate and glucosamine sulfate)	1848:1911	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	3	theme	chondroitin	1868:1878	arg1	sulfate					1880:1886	chondroitin sulfate	1868:1886	chondroitin sulfate	1868:1886	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	4	4	theme	additive/kg	753:763	arg1	0.74 g					743:748	0.74 g	743:748	0.74 g of additive/kg of feed	743:771	After hatching, the chicks from both incubation treatments were submitted to additional treatments during the growth phase from 1 to 42 D of age (diet without and with 0.74 g of additive/kg of feed).					
30982065	3	5	theme	sulfate	523:529	arg1	30 g					503:506	30 g	503:506	30 g	503:506	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	5	theme	sulfate	523:529	arg1	sulfate					523:529	chondroitin sulfate	511:529	chondroitin sulfate	511:529	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	4	6	dep	42	708:709	arg1	to					705:706	to	705:706	to	705:706	After hatching, the chicks from both incubation treatments were submitted to additional treatments during the growth phase from 1 to 42 D of age (diet without and with 0.74 g of additive/kg of feed).					
30982065	9	7	theme	dietary	1495:1501	arg1	supplementation					1503:1517	dietary supplementation	1495:1517	dietary supplementation	1495:1517	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	8	8	theme	plus	1096:1099	arg1	supplementation					1109:1123	additive plus dietary supplementation	1087:1123	additive plus dietary supplementation	1087:1123	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	10	9	theme	osteocytes	1714:1723	arg1	number					1704:1709	the number	1700:1709	the number of osteocytes	1700:1723	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	1	10	from	density	252:258	arg1	broilers					324:331	broilers	324:331	broilers	324:331	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	10	11	theme	additive	1567:1574	arg1	feeding					1542:1548	In ovo feeding	1535:1548	In ovo feeding with 4 μg of the additive	1535:1574	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	9	12	theme	phosphorus	1273:1282	arg1	percentage					1296:1305	phosphorus and calcium percentage	1273:1305	phosphorus and calcium percentage	1273:1305	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	9	12	theme	phosphorus	1273:1282	arg1	ash					1268:1270	The highest ash	1256:1270	The highest ash	1256:1270	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	11	13	theme	dietary	1796:1802	arg1	supplementation					1804:1818	dietary supplementation	1796:1818	dietary supplementation	1796:1818	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	8	14	theme	supplementation	1109:1123	arg1	4 μg					1079:1082	4 μg	1079:1082	4 μg of additive plus dietary supplementation	1079:1123	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	10	15	theme	broilers	1751:1758	arg1	diaphysis					1738:1746	the tibia diaphysis	1728:1746	the tibia diaphysis of broilers	1728:1758	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	3	16	theme	glucosamine	540:550	arg1	glucosamine					540:550	glucosamine	540:550	glucosamine	540:550	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	16	theme	glucosamine	540:550	arg1	30 g					532:535	30 g	532:535	30 g	532:535	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	1	17	from	bone	192:195	arg1	broilers					324:331	broilers	324:331	broilers	324:331	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	9	18	theme	calcium	1288:1294	arg1	percentage					1296:1305	phosphorus and calcium percentage	1273:1305	phosphorus and calcium percentage	1273:1305	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	9	18	theme	calcium	1288:1294	arg1	ash					1268:1270	The highest ash	1256:1270	The highest ash	1256:1270	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	10	19	dep	reduced	1576:1582	arg1	0.0008					1589:1594	P = 0.0008	1585:1594	P = 0.0008	1585:1594	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	10	20	theme	=	1587:1587	arg1	0.0008					1589:1594	P = 0.0008	1585:1594	P = 0.0008	1585:1594	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	5	21	contain	contained	796:804	arg2	sulfate					826:832	glucosamine sulfate	814:832	glucosamine sulfate	814:832	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	5	21	contain	contained	796:804	arg2	30 g					806:809	30 g	806:809	30 g	806:809	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	5	21	contain	contained	796:804	arg1	100 kg					781:786	Every 100 kg	775:786	Every 100 kg of feed	775:794	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	5	21	contain	contained	796:804	arg2	24 g					835:838	24 g	835:838	24 g	835:838	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	5	21	contain	contained	796:804	arg2	20 g					868:871	20 g	868:871	20 g	868:871	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	5	21	contain	contained	796:804	arg2	sulfate					855:861	chondroitin sulfate	843:861	chondroitin sulfate	843:861	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	5	21	contain	contained	796:804	arg2	C					884:884	vitamin C	876:884	vitamin C	876:884	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	4	22	theme	additional	652:661	arg1	treatments					663:672	additional treatments	652:672	additional treatments	652:672	After hatching, the chicks from both incubation treatments were submitted to additional treatments during the growth phase from 1 to 42 D of age (diet without and with 0.74 g of additive/kg of feed).					
30982065	8	23	theme	proximal	1214:1221	arg1	epiphysis					1223:1231	the femur proximal epiphysis	1204:1231	the femur proximal epiphysis in birds (P = 0.0098)	1204:1253	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	10	24	theme	chondrocytes	1611:1622	arg1	number					1601:1606	the number	1597:1606	the number of chondrocytes	1597:1622	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	5	25	theme	glucosamine	814:824	arg1	sulfate					826:832	glucosamine sulfate	814:832	glucosamine sulfate	814:832	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	8	26	theme	additive/kg	1140:1150	arg1	0.74 g					1130:1135	0.74 g	1130:1135	0.74 g of additive/kg of feed	1130:1158	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	2	27	theme	incubation	450:459	arg1	day					441:443	day 4	441:445	day 4 of incubation	441:459	Fertile eggs from breeders (Cobb) were either uninjected or injected with 4 μg of additive/100 μL water on day 4 of incubation.					
30982065	8	28	theme	feed	1155:1158	arg1	additive/kg					1140:1150	additive/kg	1140:1150	additive/kg of feed	1140:1158	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	0	29	theme	vitamin	23:29	arg1	C					31:31	vitamin C	23:31	vitamin C	23:31	Glycosaminoglycans and vitamin C affect broiler bone parameters.					
30982065	4	30	from	D	711:711	arg1	phase					692:696	the growth phase	681:696	the growth phase from 1 to 42 D of age	681:718	After hatching, the chicks from both incubation treatments were submitted to additional treatments during the growth phase from 1 to 42 D of age (diet without and with 0.74 g of additive/kg of feed).					
30982065	9	31	theme	mineral	1328:1334	arg1	composition					1336:1346	mineral composition	1328:1346	mineral composition	1328:1346	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	11	32	theme	cartilage	1970:1978	arg1	development					1940:1950	the development	1936:1950	the development of bird bones and cartilage	1936:1978	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	5	33	theme	feed	791:794	arg1	100 kg					781:786	Every 100 kg	775:786	Every 100 kg of feed	775:794	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	11	34	from	problems	2018:2025	arg1	broilers					2030:2037	broilers	2030:2037	broilers	2030:2037	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	1	35	dep	in	111:112	arg1	ovo					114:116	ovo	114:116	ovo	114:116	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	5	36	theme	sulfate	855:861	arg1	24 g					835:838	24 g	835:838	24 g	835:838	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	5	36	theme	sulfate	855:861	arg1	sulfate					855:861	chondroitin sulfate	843:861	chondroitin sulfate	843:861	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	1	37	from	histology	311:319	arg1	broilers					324:331	broilers	324:331	broilers	324:331	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	1	38	theme	study	85:89	arg1	purpose					69:75	The purpose	65:75	The purpose of this study	65:89	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	4	39	theme	growth	685:690	arg1	phase					692:696	the growth phase	681:696	the growth phase from 1 to 42 D of age	681:718	After hatching, the chicks from both incubation treatments were submitted to additional treatments during the growth phase from 1 to 42 D of age (diet without and with 0.74 g of additive/kg of feed).					
30982065	7	40	theme	model	1019:1023	arg1	procedure					1025:1033	the general linear model procedure	1000:1033	the general linear model procedure of SAS (SAS Institute)	1000:1056	The data were submitted to variance analysis using the general linear model procedure of SAS (SAS Institute).					
30982065	9	41	theme	following	1391:1399	arg1	treatments					1401:1410	the following treatments	1387:1410	the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing	1387:1532	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	9	42	from	treatments	1401:1410	arg1	ovo					1416:1418	ovo	1416:1418	ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing	1416:1532	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	11	43	with	supplementation	1804:1818	arg1	C					1925:1925	vitamin C	1917:1925	vitamin C	1917:1925	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	43	with	supplementation	1804:1818	arg1	glycosaminoglycans					1848:1865	glycosaminoglycans	1848:1865	glycosaminoglycans (chondroitin sulfate and glucosamine sulfate)	1848:1911	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	43	with	supplementation	1804:1818	arg1	sulfate					1880:1886	chondroitin sulfate	1868:1886	chondroitin sulfate	1868:1886	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	43	with	supplementation	1804:1818	arg1	sulfate					1904:1910	glucosamine sulfate	1892:1910	glucosamine sulfate	1892:1910	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	44	theme	bones	1960:1964	arg1	development					1940:1950	the development	1936:1950	the development of bird bones and cartilage	1936:1978	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	7	45	theme	general	1004:1010	arg1	procedure					1025:1033	the general linear model procedure	1000:1033	the general linear model procedure of SAS (SAS Institute)	1000:1056	The data were submitted to variance analysis using the general linear model procedure of SAS (SAS Institute).					
30982065	1	46	theme	bone	278:281	arg1	strength					292:299	bone breaking strength	278:299	bone breaking strength	278:299	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	2	47	from	breeders	352:359	arg1	eggs					342:345	Fertile eggs	334:345	Fertile eggs from breeders (Cobb)	334:366	Fertile eggs from breeders (Cobb) were either uninjected or injected with 4 μg of additive/100 μL water on day 4 of incubation.					
30982065	6	48	theme	factorial	911:919	arg1	design					921:926	A completely randomized factorial design	887:926	A completely randomized factorial design (2 × 2)	887:934	A completely randomized factorial design (2 × 2) was applied.					
30982065	6	48	theme	factorial	911:919	arg1	×					931:931	2 × 2	929:933	2 × 2	929:933	A completely randomized factorial design (2 × 2) was applied.					
30982065	2	49	theme	additive/100 μL	416:430	arg1	water					432:436	additive/100 μL water	416:436	additive/100 μL water	416:436	Fertile eggs from breeders (Cobb) were either uninjected or injected with 4 μg of additive/100 μL water on day 4 of incubation.					
30982065	10	50	dep	increased	1677:1685	arg1	<					1690:1690	P < 0.0001	1688:1697	P < 0.0001	1688:1697	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	3	51	theme	C	572:572	arg1	5 g					557:559	5 g	557:559	5 g	557:559	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	51	theme	C	572:572	arg1	C					572:572	vitamin C	564:572	vitamin C	564:572	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	0	52	theme	bone	48:51	arg1	parameters					53:62	broiler bone parameters	40:62	broiler bone parameters	40:62	Glycosaminoglycans and vitamin C affect broiler bone parameters.					
30982065	11	53	theme	vitamin	1917:1923	arg1	C					1925:1925	vitamin C	1917:1925	vitamin C	1917:1925	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	10	54	theme	proximal	1631:1638	arg1	epiphysis					1640:1648	the proximal epiphysis	1627:1648	the proximal epiphysis of the tibia cartilage	1627:1671	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	10	55	theme	cartilage	1663:1671	arg1	epiphysis					1640:1648	the proximal epiphysis	1627:1648	the proximal epiphysis of the tibia cartilage	1627:1671	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	8	56	theme	=	1245:1245	arg1	birds					1236:1240	birds	1236:1240	birds (P = 0.0098)	1236:1253	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	8	56	theme	=	1245:1245	arg1	P					1243:1243	P = 0.0098	1243:1252	P = 0.0098	1243:1252	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	10	57	with	feeding	1542:1548	arg1	4 μg					1555:1558	4 μg	1555:1558	4 μg	1555:1558	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	4	58	theme	age	716:718	arg1	phase					692:696	the growth phase	681:696	the growth phase from 1 to 42 D of age	681:718	After hatching, the chicks from both incubation treatments were submitted to additional treatments during the growth phase from 1 to 42 D of age (diet without and with 0.74 g of additive/kg of feed).					
30982065	5	59	theme	C	884:884	arg1	20 g					868:871	20 g	868:871	20 g	868:871	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	5	59	theme	C	884:884	arg1	C					884:884	vitamin C	876:884	vitamin C	876:884	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	10	60	theme	P	1688:1688	arg1	<					1690:1690	P < 0.0001	1688:1697	P < 0.0001	1688:1697	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	1	61	from	strength	292:299	arg1	broilers					324:331	broilers	324:331	broilers	324:331	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	11	62	with	feeding	1785:1791	arg1	C					1925:1925	vitamin C	1917:1925	vitamin C	1917:1925	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	62	with	feeding	1785:1791	arg1	glycosaminoglycans					1848:1865	glycosaminoglycans	1848:1865	glycosaminoglycans (chondroitin sulfate and glucosamine sulfate)	1848:1911	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	62	with	feeding	1785:1791	arg1	sulfate					1880:1886	chondroitin sulfate	1868:1886	chondroitin sulfate	1868:1886	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	62	with	feeding	1785:1791	arg1	sulfate					1904:1910	glucosamine sulfate	1892:1910	glucosamine sulfate	1892:1910	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	10	63	theme	In	1535:1536	arg1	feeding					1542:1548	In ovo feeding	1535:1548	In ovo feeding with 4 μg of the additive	1535:1574	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	9	64	theme	mineral	1308:1314	arg1	density					1316:1322	mineral density	1308:1322	mineral density	1308:1322	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	11	65	theme	glucosamine	1892:1902	arg1	glycosaminoglycans					1848:1865	glycosaminoglycans	1848:1865	glycosaminoglycans (chondroitin sulfate and glucosamine sulfate)	1848:1911	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	65	theme	glucosamine	1892:1902	arg1	sulfate					1904:1910	glucosamine sulfate	1892:1910	glucosamine sulfate	1892:1910	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	8	66	with	feeding	1066:1072	arg1	4 μg					1079:1082	4 μg	1079:1082	4 μg of additive plus dietary supplementation	1079:1123	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	8	66	with	feeding	1066:1072	arg1	0.74 g					1130:1135	0.74 g	1130:1135	0.74 g of additive/kg of feed	1130:1158	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	1	67	theme	mineral	244:250	arg1	density					252:258	mineral density	244:258	mineral density	244:258	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	10	68	theme	P	1585:1585	arg1	0.0008					1589:1594	P = 0.0008	1585:1594	P = 0.0008	1585:1594	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	11	69	dep	glycosaminoglycans	1848:1865	arg1	glycosaminoglycans					1848:1865	glycosaminoglycans	1848:1865	glycosaminoglycans (chondroitin sulfate and glucosamine sulfate)	1848:1911	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	69	dep	glycosaminoglycans	1848:1865	arg1	sulfate					1880:1886	chondroitin sulfate	1868:1886	chondroitin sulfate	1868:1886	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	69	dep	glycosaminoglycans	1848:1865	arg1	sulfate					1904:1910	glucosamine sulfate	1892:1910	glucosamine sulfate	1892:1910	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	1	70	from	area	272:275	arg1	broilers					324:331	broilers	324:331	broilers	324:331	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	3	71	theme	in	477:478	arg1	100 g					468:472	Every 100 g	462:472	Every 100 g of in ovo additive	462:491	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	8	72	from	birds	1236:1240	arg1	weight					1194:1199	the highest cartilage weight	1172:1199	the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098)	1172:1253	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	9	73	theme	uninjected	1474:1483	arg1	eggs					1485:1488	additive during rearing, or uninjected eggs	1446:1488	additive during rearing, or uninjected eggs	1446:1488	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	11	74	theme	bone	2013:2016	arg1	problems					2018:2025	bone problems	2013:2025	bone problems in broilers	2013:2037	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	3	75	theme	chondroitin	511:521	arg1	sulfate					523:529	chondroitin sulfate	511:529	chondroitin sulfate	511:529	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	8	76	from	epiphysis	1223:1231	arg1	birds					1236:1240	birds	1236:1240	birds (P = 0.0098)	1236:1253	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	8	76	from	epiphysis	1223:1231	arg1	P					1243:1243	P = 0.0098	1243:1252	P = 0.0098	1243:1252	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	1	77	theme	vitamin	166:172	arg1	C					174:174	vitamin C	166:174	vitamin C	166:174	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	11	78	theme	in	1778:1779	arg1	feeding					1785:1791	in ovo feeding	1778:1791	in ovo feeding	1778:1791	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	11	79	theme	broiler	1827:1833	arg1	rearing					1835:1841	broiler rearing	1827:1841	broiler rearing	1827:1841	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	8	80	theme	additive	1087:1094	arg1	supplementation					1109:1123	additive plus dietary supplementation	1087:1123	additive plus dietary supplementation	1087:1123	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	1	81	from	composition	231:241	arg1	broilers					324:331	broilers	324:331	broilers	324:331	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	8	82	theme	dietary	1101:1107	arg1	supplementation					1109:1123	additive plus dietary supplementation	1087:1123	additive plus dietary supplementation	1087:1123	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	3	83	dep	in	477:478	arg1	additive					484:491	additive	484:491	additive	484:491	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	83	dep	in	477:478	arg1	ovo					480:482	ovo	480:482	ovo	480:482	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	8	84	theme	In	1059:1060	arg1	feeding					1066:1072	In ovo feeding	1059:1072	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed	1059:1158	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	10	85	theme	tibia	1732:1736	arg1	diaphysis					1738:1746	the tibia diaphysis	1728:1746	the tibia diaphysis of broilers	1728:1758	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	1	86	from	cartilage	201:209	arg1	broilers					324:331	broilers	324:331	broilers	324:331	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	1	87	theme	in	111:112	arg1	feeding					118:124	in ovo feeding	111:124	in ovo feeding	111:124	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	6	88	theme	randomized	900:909	arg1	design					921:926	A completely randomized factorial design	887:926	A completely randomized factorial design (2 × 2)	887:934	A completely randomized factorial design (2 × 2) was applied.					
30982065	6	88	theme	randomized	900:909	arg1	×					931:931	2 × 2	929:933	2 × 2	929:933	A completely randomized factorial design (2 × 2) was applied.					
30982065	8	89	theme	highest	1176:1182	arg1	weight					1194:1199	the highest cartilage weight	1172:1199	the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098)	1172:1253	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	9	90	theme	highest	1260:1266	arg1	percentage					1296:1305	phosphorus and calcium percentage	1273:1305	phosphorus and calcium percentage	1273:1305	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	9	90	theme	highest	1260:1266	arg1	ash					1268:1270	The highest ash	1256:1270	The highest ash	1256:1270	The highest ash, phosphorus and calcium percentage, mineral density and mineral composition were identified for femur and tibia in the following treatments: in ovo feeding plus diet without additive during rearing, or uninjected eggs plus dietary supplementation during rearing.					
30982065	11	91	dep	in	1778:1779	arg1	ovo					1781:1783	ovo	1781:1783	ovo	1781:1783	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	1	92	theme	bone	306:309	arg1	histology					311:319	bone histology	306:319	bone histology	306:319	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	1	93	with	rearing	130:136	arg1	glycosaminoglycans					143:160	glycosaminoglycans	143:160	glycosaminoglycans	143:160	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	1	93	with	rearing	130:136	arg1	C					174:174	vitamin C	166:174	vitamin C	166:174	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	2	94	from	4 μg	408:411	arg1	day					441:443	day 4	441:445	day 4 of incubation	441:459	Fertile eggs from breeders (Cobb) were either uninjected or injected with 4 μg of additive/100 μL water on day 4 of incubation.					
30982065	4	95	theme	incubation	612:621	arg1	treatments					623:632	both incubation treatments	607:632	both incubation treatments	607:632	After hatching, the chicks from both incubation treatments were submitted to additional treatments during the growth phase from 1 to 42 D of age (diet without and with 0.74 g of additive/kg of feed).					
30982065	7	96	theme	SAS	1043:1045	arg1	SAS					1038:1040	SAS	1038:1040	SAS (SAS Institute)	1038:1056	The data were submitted to variance analysis using the general linear model procedure of SAS (SAS Institute).					
30982065	7	96	theme	SAS	1043:1045	arg1	Institute					1047:1055	SAS Institute	1043:1055	SAS Institute	1043:1055	The data were submitted to variance analysis using the general linear model procedure of SAS (SAS Institute).					
30982065	1	97	with	feeding	118:124	arg1	glycosaminoglycans					143:160	glycosaminoglycans	143:160	glycosaminoglycans	143:160	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	1	97	with	feeding	118:124	arg1	C					174:174	vitamin C	166:174	vitamin C	166:174	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	5	98	theme	chondroitin	843:853	arg1	sulfate					855:861	chondroitin sulfate	843:861	chondroitin sulfate	843:861	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	7	99	theme	SAS	1038:1040	arg1	procedure					1025:1033	the general linear model procedure	1000:1033	the general linear model procedure of SAS (SAS Institute)	1000:1056	The data were submitted to variance analysis using the general linear model procedure of SAS (SAS Institute).					
30982065	8	100	theme	cartilage	1184:1192	arg1	weight					1194:1199	the highest cartilage weight	1172:1199	the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098)	1172:1253	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	5	101	theme	sulfate	826:832	arg1	30 g					806:809	30 g	806:809	30 g	806:809	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	5	101	theme	sulfate	826:832	arg1	sulfate					826:832	glucosamine sulfate	814:832	glucosamine sulfate	814:832	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	7	102	theme	linear	1012:1017	arg1	procedure					1025:1033	the general linear model procedure	1000:1033	the general linear model procedure of SAS (SAS Institute)	1000:1056	The data were submitted to variance analysis using the general linear model procedure of SAS (SAS Institute).					
30982065	1	103	theme	surface	264:270	arg1	area					272:275	surface area	264:275	surface area	264:275	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	8	104	dep	In	1059:1060	arg1	ovo					1062:1064	ovo	1062:1064	ovo	1062:1064	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	8	105	theme	femur	1208:1212	arg1	epiphysis					1223:1231	the femur proximal epiphysis	1204:1231	the femur proximal epiphysis in birds (P = 0.0098)	1204:1253	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	0	106	theme	broiler	40:46	arg1	parameters					53:62	broiler bone parameters	40:62	broiler bone parameters	40:62	Glycosaminoglycans and vitamin C affect broiler bone parameters.					
30982065	2	107	theme	water	432:436	arg1	4 μg					408:411	4 μg	408:411	4 μg of additive/100 μL water on day 4 of incubation	408:459	Fertile eggs from breeders (Cobb) were either uninjected or injected with 4 μg of additive/100 μL water on day 4 of incubation.					
30982065	3	108	theme	vitamin	564:570	arg1	C					572:572	vitamin C	564:572	vitamin C	564:572	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	8	109	theme	epiphysis	1223:1231	arg1	weight					1194:1199	the highest cartilage weight	1172:1199	the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098)	1172:1253	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	1	110	theme	breaking	283:290	arg1	strength					292:299	bone breaking strength	278:299	bone breaking strength	278:299	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	11	111	theme	bird	1955:1958	arg1	bones					1960:1964	bird bones	1955:1964	bird bones	1955:1964	We conclude that in ovo feeding or dietary supplementation during broiler rearing with glycosaminoglycans (chondroitin sulfate and glucosamine sulfate) and vitamin C benefits the development of bird bones and cartilage, and may represent a solution to bone problems in broilers.					
30982065	3	112	contain	contained	493:501	arg2	5 g					557:559	5 g	557:559	5 g	557:559	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	112	contain	contained	493:501	arg1	100 g					468:472	Every 100 g	462:472	Every 100 g of in ovo additive	462:491	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	112	contain	contained	493:501	arg2	30 g					532:535	30 g	532:535	30 g	532:535	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	112	contain	contained	493:501	arg2	glucosamine					540:550	glucosamine	540:550	glucosamine	540:550	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	112	contain	contained	493:501	arg2	30 g					503:506	30 g	503:506	30 g	503:506	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	112	contain	contained	493:501	arg2	C					572:572	vitamin C	564:572	vitamin C	564:572	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	3	112	contain	contained	493:501	arg2	sulfate					523:529	chondroitin sulfate	511:529	chondroitin sulfate	511:529	Every 100 g of in ovo additive contained 30 g of chondroitin sulfate, 30 g of glucosamine, and 5 g of vitamin C.					
30982065	2	113	dep	either	373:378	arg1	uninjected					380:389	uninjected	380:389	uninjected	380:389	Fertile eggs from breeders (Cobb) were either uninjected or injected with 4 μg of additive/100 μL water on day 4 of incubation.					
30982065	8	114	from	weight	1194:1199	arg1	birds					1236:1240	birds	1236:1240	birds (P = 0.0098)	1236:1253	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	8	114	from	weight	1194:1199	arg1	P					1243:1243	P = 0.0098	1243:1252	P = 0.0098	1243:1252	In ovo feeding with 4 μg of additive plus dietary supplementation with 0.74 g of additive/kg of feed resulted in the highest cartilage weight of the femur proximal epiphysis in birds (P = 0.0098).					
30982065	10	115	theme	tibia	1657:1661	arg1	cartilage					1663:1671	the tibia cartilage	1653:1671	the tibia cartilage	1653:1671	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	1	116	theme	macroscopy	211:220	arg1	bone					192:195	bone	192:195	bone	192:195	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
30982065	5	117	theme	vitamin	876:882	arg1	C					884:884	vitamin C	876:884	vitamin C	876:884	Every 100 kg of feed contained 30 g of glucosamine sulfate, 24 g of chondroitin sulfate, and 20 g of vitamin C.					
30982065	10	118	dep	In	1535:1536	arg1	ovo					1538:1540	ovo	1538:1540	ovo	1538:1540	In ovo feeding with 4 μg of the additive reduced (P = 0.0008) the number of chondrocytes in the proximal epiphysis of the tibia cartilage and increased (P < 0.0001) the number of osteocytes in the tibia diaphysis of broilers.					
30982065	2	119	theme	Fertile	334:340	arg1	eggs					342:345	Fertile eggs	334:345	Fertile eggs from breeders (Cobb)	334:366	Fertile eggs from breeders (Cobb) were either uninjected or injected with 4 μg of additive/100 μL water on day 4 of incubation.					
30982065	4	120	from	treatments	623:632	arg1	chicks					595:600	the chicks	591:600	the chicks from both incubation treatments	591:632	After hatching, the chicks from both incubation treatments were submitted to additional treatments during the growth phase from 1 to 42 D of age (diet without and with 0.74 g of additive/kg of feed).					
30982065	1	121	theme	mineral	223:229	arg1	composition					231:241	mineral composition	223:241	mineral composition	223:241	The purpose of this study was to determine if in ovo feeding and rearing with glycosaminoglycans and vitamin C could influence bone and cartilage macroscopy, mineral composition, mineral density and surface area, bone breaking strength, and bone histology in broilers.					
31665674	8	0	theme	higher	980:985	arg1	intake					992:997	higher food intake	980:997	higher food intake in mice on GLU+GAL diet	980:1021	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	4	1	theme	female	452:457	arg1	mice					459:462	Weanling female mice	443:462	Weanling female mice (C57BL/6JRccHsd)	443:479	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	1	theme	female	452:457	arg1	C57BL/6JRccHsd					465:478	C57BL/6JRccHsd	465:478	C57BL/6JRccHsd	465:478	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	7	2	theme	hepatic	825:831	arg1	triglyceride					833:844	hepatic triglyceride	825:844	hepatic triglyceride (TG)	825:849	Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays.					
31665674	7	2	theme	hepatic	825:831	arg1	TG					847:848	TG	847:848	TG	847:848	Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays.					
31665674	2	3	theme	intervention	330:341	arg1	effects					306:312	the direct effects	295:312	the direct effects of this dietary intervention on the liver in the post-weaning period	295:381	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	8	4	from	diet	1018:1021	arg1	intake					992:997	higher food intake	980:997	higher food intake in mice on GLU+GAL diet	980:1021	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	0	5	from	replacement	8:18	arg1	diet					64:67	the post-weaning diet	47:67	the post-weaning diet	47:67	Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health.					
31665674	10	6	theme	GLU+GAL-fed	1302:1312	arg1	females					1314:1320	GLU+GAL-fed females	1302:1320	GLU+GAL-fed females	1302:1320	Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females.					
31665674	2	7	from	effects	306:312	arg1	liver					350:354	the liver	346:354	the liver in the post-weaning period	346:381	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	2	8	theme	dietary	322:328	arg1	intervention					330:341	this dietary intervention	317:341	this dietary intervention	317:341	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	10	9	theme	GLU-fed	1334:1340	arg1	females					1342:1348	GLU-fed females	1334:1348	GLU-fed females	1334:1348	Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females.					
31665674	4	10	theme	glucose	520:526	arg1	GLU					553:555	GLU	553:555	GLU	553:555	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	10	theme	glucose	520:526	arg1	monosaccharide					537:550	glucose (32 en%) monosaccharide	520:550	glucose (32 en%) monosaccharide (GLU)	520:556	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	0	11	theme	hepatic	92:98	arg1	health					100:105	hepatic health	92:105	hepatic health	92:105	Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health.					
31665674	9	12	theme	TG	1032:1033	arg1	content					1035:1041	Hepatic TG content	1024:1041	Hepatic TG content	1024:1041	Hepatic TG content was lower in GLU+GAL-fed than GLU-fed females, while glycogen levels were unaffected.					
31665674	12	13	theme	hepatic	1589:1595	arg1	content					1600:1606	hepatic TG content	1589:1606	hepatic TG content	1589:1606	In conclusion, replacing part of glucose with galactose in the post-weaning diet reduces hepatic TG content and hepatic inflammation.					
31665674	2	14	theme	galactose	269:277	arg1	metabolism					279:288	galactose metabolism	269:288	galactose metabolism	269:288	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	10	15	theme	mRNA	1179:1182	arg1	patterns					1159:1166	global expression patterns	1141:1166	global expression patterns of hepatic mRNA	1141:1182	Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females.					
31665674	4	16	theme	en	532:533	arg1	glucose					520:526	glucose	520:526	glucose (32 en%) monosaccharide (GLU)	520:556	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	16	theme	en	532:533	arg1	%					534:534	32 en%	529:534	32 en%	529:534	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	8	17	theme	food	987:990	arg1	intake					992:997	higher food intake	980:997	higher food intake in mice on GLU+GAL diet	980:1021	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	1	18	from	health	216:221	arg1	mice					233:236	female mice	226:236	female mice	226:236	Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice.					
31665674	7	19	theme	mRNA	792:795	arg1	expression					797:806	Global hepatic mRNA expression	777:806	Global hepatic mRNA expression	777:806	Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays.					
31665674	4	20	with	diet	510:513	arg1	GLU+GAL					615:621	GLU+GAL	615:621	GLU+GAL	615:621	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	20	with	diet	510:513	arg1	GLU					553:555	GLU	553:555	GLU	553:555	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	20	with	diet	510:513	arg1	monosaccharide					537:550	glucose (32 en%) monosaccharide	520:550	glucose (32 en%) monosaccharide (GLU)	520:556	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	20	with	diet	510:513	arg1	galactose					586:594	galactose	586:594	galactose	586:594	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	20	with	diet	510:513	arg1	glucose					574:580	glucose	574:580	glucose	574:580	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	2	21	theme	direct	299:304	arg1	effects					306:312	the direct effects	295:312	the direct effects of this dietary intervention on the liver in the post-weaning period	295:381	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	7	22	theme	glycogen	855:862	arg1	contents					864:871	hepatic triglyceride (TG) and glycogen contents	825:871	hepatic triglyceride (TG) and glycogen contents	825:871	Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays.					
31665674	1	23	from	galactose	139:147	arg1	diet					169:172	the post-weaning diet	152:172	the post-weaning diet	152:172	Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice.					
31665674	11	24	theme	acute	1461:1465	arg1	A					1495:1495	acute phase protein Serum amyloid A 3	1461:1497	acute phase protein Serum amyloid A 3	1461:1497	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	1	25	theme	post-weaning	156:167	arg1	diet					169:172	the post-weaning diet	152:172	the post-weaning diet	152:172	Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice.					
31665674	0	26	theme	health	100:105	arg1	parameters					78:87	parameters	78:87	parameters of hepatic health	78:105	Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health.					
31665674	4	27	theme	Weanling	443:450	arg1	mice					459:462	Weanling female mice	443:462	Weanling female mice (C57BL/6JRccHsd)	443:479	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	27	theme	Weanling	443:450	arg1	C57BL/6JRccHsd					465:478	C57BL/6JRccHsd	465:478	C57BL/6JRccHsd	465:478	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	11	28	theme	decreased	1427:1435	arg1	concentrations					1443:1456	decreased serum concentrations	1427:1456	decreased serum concentrations of acute phase protein Serum amyloid A 3	1427:1497	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	7	29	theme	Global	777:782	arg1	expression					797:806	Global hepatic mRNA expression	777:806	Global hepatic mRNA expression	777:806	Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays.					
31665674	2	30	theme	main	256:259	arg1	liver					243:247	The liver	239:247	The liver	239:247	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	2	30	theme	main	256:259	arg1	site					261:264	the main site	252:264	the main site of galactose metabolism	252:288	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	2	30	theme	main	256:259	arg1	metabolism					279:288	galactose metabolism	269:288	galactose metabolism	269:288	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	5	31	theme	food	660:663	arg1	intake					665:670	food intake	660:670	food intake	660:670	Body weight, body composition, and food intake were determined weekly.					
31665674	11	32	from	reduction	1356:1364	arg1	females					1397:1403	GLU+GAL-fed females	1385:1403	GLU+GAL-fed females	1385:1403	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	11	32	from	reduction	1356:1364	arg1	inflammation					1369:1380	inflammation	1369:1380	inflammation	1369:1380	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	8	33	theme	Body	910:913	arg1	weight					915:920	Body weight	910:920	Body weight	910:920	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	7	34	theme	hepatic	784:790	arg1	expression					797:806	Global hepatic mRNA expression	777:806	Global hepatic mRNA expression	777:806	Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays.					
31665674	0	35	theme	Partial	0:6	arg1	replacement					8:18	Partial replacement	0:18	Partial replacement of glucose by galactose in the post-weaning diet	0:67	Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health.					
31665674	4	36	theme	containing	499:508	arg1	diet					510:513	a starch containing diet	490:513	a starch containing diet with glucose (32 en%) monosaccharide (GLU)	490:556	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	37	theme	en	609:610	arg1	%					611:611	16 en%	606:611	16 en%	606:611	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	9	38	theme	GLU-fed	1073:1079	arg1	females					1081:1087	GLU-fed females	1073:1087	GLU-fed females	1073:1087	Hepatic TG content was lower in GLU+GAL-fed than GLU-fed females, while glycogen levels were unaffected.					
31665674	8	39	from	groups	964:969	arg1	similar					948:954	similar	948:954	similar	948:954	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	0	40	theme	glucose	23:29	arg1	replacement					8:18	Partial replacement	0:18	Partial replacement of glucose by galactose in the post-weaning diet	0:67	Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health.					
31665674	4	41	theme	starch	492:497	arg1	diet					510:513	a starch containing diet	490:513	a starch containing diet with glucose (32 en%) monosaccharide (GLU)	490:556	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	11	42	theme	A	1495:1495	arg1	concentrations					1443:1456	decreased serum concentrations	1427:1456	decreased serum concentrations of acute phase protein Serum amyloid A 3	1427:1497	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	10	43	theme	inflammation-related	1203:1222	arg1	pathways					1224:1231	mainly inflammation-related pathways	1196:1231	mainly inflammation-related pathways	1196:1231	Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females.					
31665674	12	44	theme	hepatic	1612:1618	arg1	inflammation					1620:1631	hepatic inflammation	1612:1631	hepatic inflammation	1612:1631	In conclusion, replacing part of glucose with galactose in the post-weaning diet reduces hepatic TG content and hepatic inflammation.					
31665674	1	45	theme	later	195:199	arg1	health					216:221	later life metabolic health	195:221	later life metabolic health in female mice	195:236	Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice.					
31665674	11	46	theme	amyloid	1487:1493	arg1	A					1495:1495	acute phase protein Serum amyloid A 3	1461:1497	acute phase protein Serum amyloid A 3	1461:1497	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	11	47	theme	serum	1437:1441	arg1	concentrations					1443:1456	decreased serum concentrations	1427:1456	decreased serum concentrations of acute phase protein Serum amyloid A 3	1427:1497	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	2	48	theme	metabolism	279:288	arg1	liver					243:247	The liver	239:247	The liver	239:247	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	2	48	theme	metabolism	279:288	arg1	site					261:264	the main site	252:264	the main site of galactose metabolism	252:288	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	2	48	theme	metabolism	279:288	arg1	metabolism					279:288	galactose metabolism	269:288	galactose metabolism	269:288	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	1	49	theme	life	201:204	arg1	health					216:221	later life metabolic health	195:221	later life metabolic health in female mice	195:236	Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice.					
31665674	5	50	theme	body	638:641	arg1	composition					643:653	body composition	638:653	body composition	638:653	Body weight, body composition, and food intake were determined weekly.					
31665674	7	51	theme	enzymatic	892:900	arg1	assays					902:907	enzymatic assays	892:907	enzymatic assays	892:907	Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays.					
31665674	11	52	theme	Serum	1481:1485	arg1	A					1495:1495	acute phase protein Serum amyloid A 3	1461:1497	acute phase protein Serum amyloid A 3	1461:1497	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	12	53	theme	glucose	1533:1539	arg1	part					1525:1528	part	1525:1528	part of glucose	1525:1539	In conclusion, replacing part of glucose with galactose in the post-weaning diet reduces hepatic TG content and hepatic inflammation.					
31665674	1	54	theme	metabolic	206:214	arg1	health					216:221	later life metabolic health	195:221	later life metabolic health in female mice	195:236	Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice.					
31665674	2	55	theme	post-weaning	363:374	arg1	period					376:381	the post-weaning period	359:381	the post-weaning period	359:381	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	8	56	theme	body	926:929	arg1	composition					931:941	body composition	926:941	body composition	926:941	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	4	57	with	diet	564:567	arg1	GLU+GAL					615:621	GLU+GAL	615:621	GLU+GAL	615:621	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	57	with	diet	564:567	arg1	GLU					553:555	GLU	553:555	GLU	553:555	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	57	with	diet	564:567	arg1	monosaccharide					537:550	glucose (32 en%) monosaccharide	520:550	glucose (32 en%) monosaccharide (GLU)	520:556	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	57	with	diet	564:567	arg1	galactose					586:594	galactose	586:594	galactose	586:594	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	4	57	with	diet	564:567	arg1	glucose					574:580	glucose	574:580	glucose	574:580	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	10	58	theme	hepatic	1171:1177	arg1	mRNA					1179:1182	hepatic mRNA	1171:1182	hepatic mRNA	1171:1182	Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females.					
31665674	3	59	theme	study	414:418	arg1	aim					402:404	The aim	398:404	The aim of this study	398:418	The aim of this study was to elucidate this.					
31665674	5	60	theme	Body	625:628	arg1	weight					630:635	Body weight	625:635	Body weight	625:635	Body weight, body composition, and food intake were determined weekly.					
31665674	2	61	from	liver	350:354	arg1	period					376:381	the post-weaning period	359:381	the post-weaning period	359:381	The liver is the main site of galactose metabolism, but the direct effects of this dietary intervention on the liver in the post-weaning period are not known.					
31665674	8	62	from	mice	1002:1005	arg1	diet					1018:1021	GLU+GAL diet	1010:1021	GLU+GAL diet	1010:1021	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	10	63	theme	patterns	1159:1166	arg1	Analysis					1129:1136	Analysis	1129:1136	Analysis of global expression patterns of hepatic mRNA	1129:1182	Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females.					
31665674	12	64	theme	TG	1597:1598	arg1	content					1600:1606	hepatic TG content	1589:1606	hepatic TG content	1589:1606	In conclusion, replacing part of glucose with galactose in the post-weaning diet reduces hepatic TG content and hepatic inflammation.					
31665674	9	65	theme	Hepatic	1024:1030	arg1	content					1035:1041	Hepatic TG content	1024:1041	Hepatic TG content	1024:1041	Hepatic TG content was lower in GLU+GAL-fed than GLU-fed females, while glycogen levels were unaffected.					
31665674	4	66	dep	glucose	574:580	arg1	1:1					597:599	1:1	597:599	1:1	597:599	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	10	67	theme	expression	1148:1157	arg1	patterns					1159:1166	global expression patterns	1141:1166	global expression patterns of hepatic mRNA	1141:1182	Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females.					
31665674	8	68	from	similar	948:954	arg1	groups					964:969	both groups	959:969	both groups	959:969	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	8	69	from	intake	992:997	arg1	diet					1018:1021	GLU+GAL diet	1010:1021	GLU+GAL diet	1010:1021	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	8	69	from	intake	992:997	arg1	mice					1002:1005	mice	1002:1005	mice on GLU+GAL diet	1002:1021	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	4	70	dep	1:1	597:599	arg1	both					601:604	both	601:604	both	601:604	Weanling female mice (C57BL/6JRccHsd) were fed a starch containing diet with glucose (32 en%) monosaccharide (GLU), or a diet with glucose and galactose (1:1 both 16 en%) (GLU+GAL).					
31665674	7	71	theme	triglyceride	833:844	arg1	contents					864:871	hepatic triglyceride (TG) and glycogen contents	825:871	hepatic triglyceride (TG) and glycogen contents	825:871	Global hepatic mRNA expression was analyzed and hepatic triglyceride (TG) and glycogen contents were determined by enzymatic assays.					
31665674	1	72	theme	glucose	126:132	arg1	part					118:121	part	118:121	part of glucose	118:132	Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice.					
31665674	1	73	theme	female	226:231	arg1	mice					233:236	female mice	226:236	female mice	226:236	Replacing part of glucose with galactose in the post-weaning diet beneficially affects later life metabolic health in female mice.					
31665674	10	74	theme	global	1141:1146	arg1	patterns					1159:1166	global expression patterns	1141:1166	global expression patterns of hepatic mRNA	1141:1182	Analysis of global expression patterns of hepatic mRNA showed that mainly inflammation-related pathways were affected by the diet, which were predominantly downregulated in GLU+GAL-fed females compared to GLU-fed females.					
31665674	12	75	theme	post-weaning	1563:1574	arg1	diet					1576:1579	the post-weaning diet	1559:1579	the post-weaning diet	1559:1579	In conclusion, replacing part of glucose with galactose in the post-weaning diet reduces hepatic TG content and hepatic inflammation.					
31665674	6	76	dep	serum	737:741	arg1	tissues					753:759	tissues	753:759	tissues	753:759	After 3 weeks, mice were sacrificed, and serum and liver tissues were collected.					
31665674	0	77	theme	post-weaning	51:62	arg1	diet					64:67	the post-weaning diet	47:67	the post-weaning diet	47:67	Partial replacement of glucose by galactose in the post-weaning diet improves parameters of hepatic health.					
31665674	9	78	theme	glycogen	1096:1103	arg1	levels					1105:1110	glycogen levels	1096:1110	glycogen levels	1096:1110	Hepatic TG content was lower in GLU+GAL-fed than GLU-fed females, while glycogen levels were unaffected.					
31665674	11	79	theme	phase	1467:1471	arg1	A					1495:1495	acute phase protein Serum amyloid A 3	1461:1497	acute phase protein Serum amyloid A 3	1461:1497	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	8	80	theme	GLU+GAL	1010:1016	arg1	diet					1018:1021	GLU+GAL diet	1010:1021	GLU+GAL diet	1010:1021	Body weight and body composition were similar in both groups, despite higher food intake in mice on GLU+GAL diet.					
31665674	11	81	theme	protein	1473:1479	arg1	A					1495:1495	acute phase protein Serum amyloid A 3	1461:1497	acute phase protein Serum amyloid A 3	1461:1497	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
31665674	11	82	theme	GLU+GAL-fed	1385:1395	arg1	females					1397:1403	GLU+GAL-fed females	1385:1403	GLU+GAL-fed females	1385:1403	This reduction in inflammation in GLU+GAL-fed females was also reflected by decreased serum concentrations of acute phase protein Serum amyloid A 3.					
30518374	2	0	from	exposure	452:459	arg1	pups					468:471	rat pups	464:471	rat pups	464:471	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	2	1	theme	neonatal	372:379	arg1	exposure					452:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure	372:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups	372:471	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	2	2	gly	glycoproteins	532:544	arg1	glycoproteins					532:544	cerebral glycoproteins	523:544	cerebral glycoproteins	523:544	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	4	3	theme	glycoproteins	842:854	arg1	sialylation					818:828	sialylation	818:828	sialylation of cerebral glycoproteins and glycolipids	818:870	Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.					
30518374	4	4	theme	glycolipids	860:870	arg1	sialylation					818:828	sialylation	818:828	sialylation of cerebral glycoproteins and glycolipids	818:870	Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.					
30518374	7	5	from	sialylation	1239:1249	arg1	glycoproteins					1273:1285	brain glycoproteins	1267:1285	brain glycoproteins	1267:1285	At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.					
30518374	10	6	theme	sialome	1953:1959	arg1	re-programming					1925:1938	observed re-programming	1916:1938	observed re-programming of the brain sialome	1916:1959	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	3	7	theme	Male	555:558	arg1	pups					579:582	Male Sprague-Dawley rat pups	555:582	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3)	547:606	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3) were injected in the corpus callosum with saline or LPS.					
30518374	4	8	gly	glycoproteins	842:854	arg1	glycoproteins					842:854	cerebral glycoproteins	833:854	cerebral glycoproteins	833:854	Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.					
30518374	10	9	theme	pathophysiological	1994:2011	arg1	consequences					2013:2024	pathophysiological consequences	1994:2024	pathophysiological consequences	1994:2024	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	8	10	from	neurons	1491:1497	arg1	layers					1512:1517	the upper layers	1502:1517	the upper layers (II-III) of somatosensory cortex	1502:1550	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	8	10	from	neurons	1491:1497	arg1	II-III					1520:1525	II-III	1520:1525	II-III	1520:1525	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	6	11	theme	activity	1209:1216	arg1	rise					1187:1190	the rise	1183:1190	the rise of neuraminidase activity	1183:1216	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	9	12	theme	Neu1	1719:1722	arg1	induction					1706:1714	specific and sustained induction	1683:1714	specific and sustained induction of Neu1 and Neu4	1683:1731	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	8	13	theme	tissues	1447:1453	arg1	analysis					1429:1436	analysis	1429:1436	analysis of brain tissues by immunohistochemistry	1429:1477	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	7	14	theme	lectin	1346:1351	arg1	blotting					1353:1360	lectin blotting	1346:1360	lectin blotting	1346:1360	At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.					
30518374	10	15	theme	infectious	2039:2048	arg1	exposure					2050:2057	perinatal infectious exposure	2029:2057	perinatal infectious exposure	2029:2057	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	5	16	theme	significant	915:925	arg1	increase					927:934	a significant increase	913:934	a significant increase of the acidic neuraminidase activity in LPS-exposed rats	913:991	RESULTS At both P4 and P24, we detected a significant increase of the acidic neuraminidase activity in LPS-exposed rats.					
30518374	6	17	theme	increased	1027:1035	arg1	mRNA					1060:1063	significantly increased neuraminidase 1 (Neu1) mRNA	1013:1063	significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4	1013:1091	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	9	18	theme	Neu4	1728:1731	arg1	induction					1706:1714	specific and sustained induction	1683:1714	specific and sustained induction of Neu1 and Neu4	1683:1731	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	0	19	theme	postnatal	72:80	arg1	exposure					95:102	postnatal inflammatory exposure	72:102	postnatal inflammatory exposure	72:102	Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.					
30518374	9	20	theme	brain	1807:1811	arg1	cells					1813:1817	brain cells	1807:1817	brain cells	1807:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	9	21	gly	sialylation	1775:1785	arg1	glycoproteins					1790:1802	glycoproteins	1790:1802	glycoproteins	1790:1802	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	9	21	gly	sialylation	1775:1785	arg1	cells					1813:1817	brain cells	1807:1817	brain cells	1807:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	2	22	theme	cerebral	523:530	arg1	glycoproteins					532:544	cerebral glycoproteins	523:544	cerebral glycoproteins	523:544	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	1	23	dep	BACKGROUND	105:114	arg1	leads					274:278	leads	274:278	leads to substantial long-term neurodevelopmental disabilities	274:335	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	1	23	dep	BACKGROUND	105:114	arg1	overlooked					190:199	overlooked	190:199	is often overlooked due to technical challenges in this highly vulnerable population	181:264	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	7	24	gly	glycoproteins	1273:1285	arg1	glycoproteins					1273:1285	brain glycoproteins	1267:1285	brain glycoproteins	1267:1285	At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.					
30518374	9	25	gly	glycoproteins	1790:1802	arg1	glycoproteins					1790:1802	glycoproteins	1790:1802	glycoproteins	1790:1802	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	9	26	theme	negative	1755:1762	arg1	changes					1764:1770	long-lasting negative changes	1742:1770	long-lasting negative changes in sialylation of glycoproteins on brain cells	1742:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	3	27	from	day	597:599	arg1	pups					579:582	Male Sprague-Dawley rat pups	555:582	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3)	547:606	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3) were injected in the corpus callosum with saline or LPS.					
30518374	5	28	theme	activity	964:971	arg1	increase					927:934	a significant increase	913:934	a significant increase of the acidic neuraminidase activity in LPS-exposed rats	913:991	RESULTS At both P4 and P24, we detected a significant increase of the acidic neuraminidase activity in LPS-exposed rats.					
30518374	1	29	theme	preterm	157:163	arg1	brain					175:179	the preterm newborn's brain	153:179	the preterm newborn's brain	153:179	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	8	30	theme	upper	1506:1510	arg1	layers					1512:1517	the upper layers	1502:1517	the upper layers (II-III) of somatosensory cortex	1502:1550	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	8	30	theme	upper	1506:1510	arg1	II-III					1520:1525	II-III	1520:1525	II-III	1520:1525	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	5	31	theme	LPS-exposed	976:986	arg1	rats					988:991	LPS-exposed rats	976:991	LPS-exposed rats	976:991	RESULTS At both P4 and P24, we detected a significant increase of the acidic neuraminidase activity in LPS-exposed rats.					
30518374	7	32	theme	mass-spectrometry	1315:1331	arg1	analysis					1333:1340	mass-spectrometry analysis	1315:1340	mass-spectrometry analysis	1315:1340	At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.					
30518374	2	33	theme	coli	422:425	arg1	lipopolysaccharide					427:444	E. coli lipopolysaccharide	419:444	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups	372:471	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	2	33	theme	coli	422:425	arg1	LPS					447:449	LPS	447:449	LPS	447:449	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	0	34	theme	Persistent	0:9	arg1	reduction					11:19	Persistent reduction	0:19	Persistent reduction in sialylation of cerebral glycoproteins	0:60	Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.					
30518374	1	35	theme	substantial	283:293	arg1	disabilities					324:335	substantial long-term neurodevelopmental disabilities	283:335	substantial long-term neurodevelopmental disabilities	283:335	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	9	36	theme	neonatal	1650:1657	arg1	exposure					1663:1670	neonatal LPS exposure	1650:1670	neonatal LPS exposure	1650:1670	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	1	37	theme	neurodevelopmental	305:322	arg1	disabilities					324:335	substantial long-term neurodevelopmental disabilities	283:335	substantial long-term neurodevelopmental disabilities	283:335	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	5	38	theme	acidic	943:948	arg1	activity					964:971	the acidic neuraminidase activity	939:971	the acidic neuraminidase activity	939:971	RESULTS At both P4 and P24, we detected a significant increase of the acidic neuraminidase activity in LPS-exposed rats.					
30518374	6	39	theme	Neu1	1054:1057	arg1	mRNA					1060:1063	significantly increased neuraminidase 1 (Neu1) mRNA	1013:1063	significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4	1013:1091	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	8	40	theme	somatosensory	1531:1543	arg1	cortex					1545:1550	somatosensory cortex	1531:1550	somatosensory cortex	1531:1550	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	2	41	gly	sialylation	508:518	arg1	glycoproteins					532:544	cerebral glycoproteins	523:544	cerebral glycoproteins	523:544	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	7	42	theme	brain	1267:1271	arg1	glycoproteins					1273:1285	brain glycoproteins	1267:1285	brain glycoproteins	1267:1285	At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.					
30518374	10	43	theme	important	1836:1844	arg1	roles					1846:1850	the important roles	1832:1850	the important roles played by sialoglycoproteins in CNS function	1832:1895	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	0	44	theme	glycoproteins	48:60	arg1	sialylation					24:34	sialylation	24:34	sialylation of cerebral glycoproteins	24:60	Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.					
30518374	9	45	theme	LPS	1659:1661	arg1	exposure					1663:1670	neonatal LPS exposure	1650:1670	neonatal LPS exposure	1650:1670	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	7	46	theme	N-glycans	1254:1262	arg1	sialylation					1239:1249	sialylation	1239:1249	sialylation of N-glycans on brain glycoproteins	1239:1285	At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.					
30518374	8	47	theme	reduced	1558:1564	arg1	content					1574:1580	a reduced surface content	1556:1580	a reduced surface content of polysialic acid	1556:1599	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	10	48	theme	CNS	1884:1886	arg1	function					1888:1895	CNS function	1884:1895	CNS function	1884:1895	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	3	49	theme	Sprague-Dawley	560:573	arg1	pups					579:582	Male Sprague-Dawley rat pups	555:582	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3)	547:606	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3) were injected in the corpus callosum with saline or LPS.					
30518374	4	50	dep	hours	677:681	arg1	following					705:713	following	705:713	following	705:713	Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.					
30518374	4	50	dep	hours	677:681	arg1	injection					715:723	injection	715:723	injection	715:723	Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.					
30518374	8	51	theme	polysialic	1585:1594	arg1	acid					1596:1599	polysialic acid	1585:1599	polysialic acid	1585:1599	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	2	52	theme	neuroinflammation	381:397	arg1	exposure					452:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure	372:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups	372:471	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	9	53	from	cells	1813:1817	arg1	changes					1764:1770	long-lasting negative changes	1742:1770	long-lasting negative changes in sialylation of glycoproteins on brain cells	1742:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	6	54	from	mRNA	1060:1063	arg1	P4					1090:1091	P4	1090:1091	P4	1090:1091	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	6	54	from	mRNA	1060:1063	arg1	brains					1080:1085	LPS-treated brains	1068:1085	LPS-treated brains at P4	1068:1091	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	3	55	theme	postnatal	587:595	arg1	day					597:599	postnatal day 3	587:601	postnatal day 3 (P3)	587:606	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3) were injected in the corpus callosum with saline or LPS.					
30518374	3	55	theme	postnatal	587:595	arg1	P3					604:605	P3	604:605	P3	604:605	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3) were injected in the corpus callosum with saline or LPS.					
30518374	4	56	theme	cerebral	833:840	arg1	glycoproteins					842:854	cerebral glycoproteins	833:854	cerebral glycoproteins	833:854	Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.					
30518374	10	57	theme	observed	1916:1923	arg1	re-programming					1925:1938	observed re-programming	1916:1938	observed re-programming of the brain sialome	1916:1959	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	10	58	gly	sialoglycoproteins	1862:1879	arg1	sialoglycoproteins					1862:1879	sialoglycoproteins	1862:1879	sialoglycoproteins	1862:1879	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	0	59	gly	sialylation	24:34	arg1	glycoproteins					48:60	cerebral glycoproteins	39:60	cerebral glycoproteins	39:60	Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.					
30518374	1	60	theme	technical	208:216	arg1	challenges					218:227	technical challenges	208:227	technical challenges	208:227	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	9	61	theme	specific	1683:1690	arg1	induction					1706:1714	specific and sustained induction	1683:1714	specific and sustained induction of Neu1 and Neu4	1683:1731	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	10	62	theme	brain	1947:1951	arg1	sialome					1953:1959	the brain sialome	1943:1959	the brain sialome	1943:1959	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	9	63	theme	sustained	1696:1704	arg1	induction					1706:1714	specific and sustained induction	1683:1714	specific and sustained induction of Neu1 and Neu4	1683:1731	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	8	64	theme	brain	1441:1445	arg1	tissues					1447:1453	brain tissues	1441:1453	brain tissues	1441:1453	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	7	65	theme	ganglioside	1371:1381	arg1	composition					1383:1393	the ganglioside composition	1367:1393	the ganglioside composition	1367:1393	At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.					
30518374	9	66	dep	CONCLUSIONS	1602:1612	arg1	demonstrate					1633:1643	demonstrate	1633:1643	demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells	1633:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	1	67	theme	sepsis	133:138	arg1	extension					120:128	The extension	116:128	The extension of sepsis encompassing the preterm newborn's brain	116:179	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	6	68	theme	neuraminidase	1195:1207	arg1	activity					1209:1216	neuraminidase activity	1195:1216	neuraminidase activity	1195:1216	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	8	69	contain	had	1552:1554	arg2	content					1574:1580	a reduced surface content	1556:1580	a reduced surface content of polysialic acid	1556:1599	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	8	69	contain	had	1552:1554	arg1	neurons					1491:1497	neurons	1491:1497	neurons in the upper layers (II-III) of somatosensory cortex	1491:1550	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	10	70	theme	important	1976:1984	arg1	part					1986:1989	an important part	1973:1989	an important part of pathophysiological consequences	1973:2024	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	6	71	theme	neuraminidase	1037:1049	arg1	mRNA					1060:1063	significantly increased neuraminidase 1 (Neu1) mRNA	1013:1063	significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4	1013:1091	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	10	72	theme	perinatal	2029:2037	arg1	exposure					2050:2057	perinatal infectious exposure	2029:2057	perinatal infectious exposure	2029:2057	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	0	73	theme	inflammatory	82:93	arg1	exposure					95:102	postnatal inflammatory exposure	72:102	postnatal inflammatory exposure	72:102	Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.					
30518374	3	74	theme	corpus	629:634	arg1	callosum					636:643	the corpus callosum	625:643	the corpus callosum	625:643	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3) were injected in the corpus callosum with saline or LPS.					
30518374	1	75	theme	newborn	165:171	arg1	brain					175:179	the preterm newborn's brain	153:179	the preterm newborn's brain	153:179	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	2	76	theme	glycoproteins	532:544	arg1	sialylation					508:518	sialylation	508:518	sialylation of cerebral glycoproteins	508:544	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	10	77	theme	consequences	2013:2024	arg1	part					1986:1989	an important part	1973:1989	an important part of pathophysiological consequences	1973:2024	Considering the important roles played by sialoglycoproteins in CNS function, we speculate that observed re-programming of the brain sialome constitutes an important part of pathophysiological consequences in perinatal infectious exposure.					
30518374	9	78	theme	long-lasting	1742:1753	arg1	changes					1764:1770	long-lasting negative changes	1742:1770	long-lasting negative changes in sialylation of glycoproteins on brain cells	1742:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	0	79	from	reduction	11:19	arg1	sialylation					24:34	sialylation	24:34	sialylation of cerebral glycoproteins	24:60	Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.					
30518374	5	80	theme	neuraminidase	950:962	arg1	activity					964:971	the acidic neuraminidase activity	939:971	the acidic neuraminidase activity	939:971	RESULTS At both P4 and P24, we detected a significant increase of the acidic neuraminidase activity in LPS-exposed rats.					
30518374	2	81	from	reduction	495:503	arg1	sialylation					508:518	sialylation	508:518	sialylation of cerebral glycoproteins	508:544	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	4	82	theme	neuraminidase	765:777	arg1	activity					779:786	neuraminidase activity	765:786	neuraminidase activity	765:786	Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.					
30518374	2	83	theme	lipopolysaccharide	427:444	arg1	exposure					452:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure	372:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups	372:471	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	3	84	dep	METHODS	547:553	arg1	pups					579:582	Male Sprague-Dawley rat pups	555:582	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3)	547:606	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3) were injected in the corpus callosum with saline or LPS.					
30518374	9	85	theme	glycoproteins	1790:1802	arg1	sialylation					1775:1785	sialylation	1775:1785	sialylation of glycoproteins on brain cells	1775:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	6	86	from	P24	1128:1130	arg1	neuraminidases					1102:1115	neuraminidases 1 and 4	1102:1123	neuraminidases 1 and 4 at P24	1102:1130	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	2	87	theme	E.	419:420	arg1	lipopolysaccharide					427:444	E. coli lipopolysaccharide	419:444	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups	372:471	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	2	87	theme	E.	419:420	arg1	LPS					447:449	LPS	447:449	LPS	447:449	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	4	88	gly	sialylation	818:828	arg1	glycolipids					860:870	glycolipids	860:870	glycolipids	860:870	Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.					
30518374	4	88	gly	sialylation	818:828	arg1	glycoproteins					842:854	cerebral glycoproteins	833:854	cerebral glycoproteins	833:854	Twenty-four hours (P4) or 21 days (P24) following injection, brains were extracted and analyzed for neuraminidase activity and expression as well as for sialylation of cerebral glycoproteins and glycolipids.					
30518374	1	89	theme	long-term	295:303	arg1	disabilities					324:335	substantial long-term neurodevelopmental disabilities	283:335	substantial long-term neurodevelopmental disabilities	283:335	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	2	90	theme	rat	464:466	arg1	pups					468:471	rat pups	464:471	rat pups	464:471	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	8	91	theme	cortex	1545:1550	arg1	layers					1512:1517	the upper layers	1502:1517	the upper layers (II-III) of somatosensory cortex	1502:1550	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	8	91	theme	cortex	1545:1550	arg1	II-III					1520:1525	II-III	1520:1525	II-III	1520:1525	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	2	92	theme	postnatal	409:417	arg1	exposure					452:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure	372:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups	372:471	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	0	93	theme	cerebral	39:46	arg1	glycoproteins					48:60	cerebral glycoproteins	39:60	cerebral glycoproteins	39:60	Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.					
30518374	9	94	from	sialylation	1775:1785	arg1	cells					1813:1817	brain cells	1807:1817	brain cells	1807:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	9	95	from	changes	1764:1770	arg1	cells					1813:1817	brain cells	1807:1817	brain cells	1807:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	9	95	from	changes	1764:1770	arg1	sialylation					1775:1785	sialylation	1775:1785	sialylation of glycoproteins on brain cells	1775:1817	CONCLUSIONS Together, our data demonstrate that neonatal LPS exposure results in specific and sustained induction of Neu1 and Neu4, causing long-lasting negative changes in sialylation of glycoproteins on brain cells.					
30518374	2	96	theme	persistent	484:493	arg1	reduction					495:503	persistent reduction	484:503	persistent reduction in sialylation of cerebral glycoproteins	484:544	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	8	97	theme	surface	1566:1572	arg1	content					1574:1580	a reduced surface content	1556:1580	a reduced surface content of polysialic acid	1556:1599	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	5	98	dep	RESULTS	873:879	arg1	detected					904:911	detected	904:911	detected a significant increase of the acidic neuraminidase activity in LPS-exposed rats	904:991	RESULTS At both P4 and P24, we detected a significant increase of the acidic neuraminidase activity in LPS-exposed rats.					
30518374	7	99	gly	sialylation	1239:1249	arg1	glycoproteins					1273:1285	brain glycoproteins	1267:1285	brain glycoproteins	1267:1285	At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.					
30518374	7	99	gly	sialylation	1239:1249	arg1	N-glycans					1254:1262	N-glycans	1254:1262	N-glycans	1254:1262	At both P4 and P24, sialylation of N-glycans on brain glycoproteins decreased according to both mass-spectrometry analysis and lectin blotting, but the ganglioside composition remained intact.					
30518374	6	100	from	P4	1090:1091	arg1	mRNA					1060:1063	significantly increased neuraminidase 1 (Neu1) mRNA	1013:1063	significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4	1013:1091	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	6	100	from	P4	1090:1091	arg1	brains					1080:1085	LPS-treated brains	1068:1085	LPS-treated brains at P4	1068:1091	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	2	101	theme	following	399:407	arg1	exposure					452:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure	372:459	neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups	372:471	In this study, we demonstrate how neonatal neuroinflammation following postnatal E. coli lipopolysaccharide (LPS) exposure in rat pups results in persistent reduction in sialylation of cerebral glycoproteins.					
30518374	5	102	from	increase	927:934	arg1	rats					988:991	LPS-exposed rats	976:991	LPS-exposed rats	976:991	RESULTS At both P4 and P24, we detected a significant increase of the acidic neuraminidase activity in LPS-exposed rats.					
30518374	1	103	theme	vulnerable	244:253	arg1	population					255:264	this highly vulnerable population	232:264	this highly vulnerable population	232:264	BACKGROUND The extension of sepsis encompassing the preterm newborn's brain is often overlooked due to technical challenges in this highly vulnerable population, yet it leads to substantial long-term neurodevelopmental disabilities.					
30518374	0	104	gly	glycoproteins	48:60	arg1	glycoproteins					48:60	cerebral glycoproteins	39:60	cerebral glycoproteins	39:60	Persistent reduction in sialylation of cerebral glycoproteins following postnatal inflammatory exposure.					
30518374	6	105	theme	LPS-treated	1068:1078	arg1	brains					1080:1085	LPS-treated brains	1068:1085	LPS-treated brains at P4	1068:1091	It correlated with significantly increased neuraminidase 1 (Neu1) mRNA in LPS-treated brains at P4 and with neuraminidases 1 and 4 at P24 suggesting that these enzymes were responsible for the rise of neuraminidase activity.					
30518374	8	106	theme	acid	1596:1599	arg1	content					1574:1580	a reduced surface content	1556:1580	a reduced surface content of polysialic acid	1556:1599	Finally, at P24, analysis of brain tissues by immunohistochemistry showed that neurons in the upper layers (II-III) of somatosensory cortex had a reduced surface content of polysialic acid.					
30518374	3	107	theme	rat	575:577	arg1	pups					579:582	Male Sprague-Dawley rat pups	555:582	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3)	547:606	METHODS Male Sprague-Dawley rat pups at postnatal day 3 (P3) were injected in the corpus callosum with saline or LPS.					
30659858	5	0	theme	untreated	767:775	arg1	hardwoods					777:785	untreated hardwoods	767:785	untreated hardwoods	767:785	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	6	1	theme	cellulose	934:942	arg1	units					917:921	guaiacyl units	908:921	guaiacyl units of lignin, cellulose and hemicelluloses	908:961	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	1	2	theme	validate	156:163	arg1	techniques					179:188	fast, validate and sensitive techniques	150:188	fast, validate and sensitive techniques	150:188	Evaluating the biomass degradation using fast, validate and sensitive techniques for exploratory purposes of biofuel production has been developed since last decade.					
30659858	7	3	theme	hemicelluloses	1073:1086	arg1	units					1053:1057	guaiacyl- and syringyl- units	1029:1057	guaiacyl- and syringyl- units of lignin and hemicelluloses	1029:1086	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	1	4	theme	last	262:265	arg1	decade					267:272	last decade	262:272	last decade	262:272	Evaluating the biomass degradation using fast, validate and sensitive techniques for exploratory purposes of biofuel production has been developed since last decade.					
30659858	5	5	dep	P.	707:708	arg1	acaciicola					710:719	acaciicola	710:719	acaciicola	710:719	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	3	6	theme	hardwood	528:535	arg1	degradation					537:547	higher hardwood degradation	521:547	higher hardwood degradation	521:547	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	7	7	dep	T.	964:965	arg1	giganteum					967:975	giganteum	967:975	giganteum	967:975	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	4	8	theme	T.	592:593	arg1	AGDR1					605:609	T. giganteum AGDR1	592:609	T. giganteum AGDR1	592:609	In the screening, P. acaciicola AGST3 and T. giganteum AGDR1 depicted the dry woody mass loss of 20.51% and 22.38%, respectively.					
30659858	5	9	theme	treated	727:733	arg1	hardwoods					735:743	P. acaciicola AGST3 treated hardwoods	707:743	P. acaciicola AGST3 treated hardwoods	707:743	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	6	10	theme	lignin	926:931	arg1	units					917:921	guaiacyl units	908:921	guaiacyl units of lignin, cellulose and hemicelluloses	908:961	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	6	11	theme	maximum	887:893	arg1	deviation					895:903	maximum deviation	887:903	maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses	887:961	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	0	12	theme	multivariate	86:97	arg1	analysis					99:106	multivariate analysis	86:106	multivariate analysis	86:106	Assessment of white rot fungus mediated hardwood degradation by FTIR spectroscopy and multivariate analysis.					
30659858	8	13	dep	degradation	1124:1134	arg1	varied					1165:1170	varied	1165:1170	varied	1165:1170	Multivariate approach revealed the degradation patterns and preferences are varied based on the fungi and hardwood.					
30659858	10	14	theme	fungi	1405:1409	arg1	degradability					1382:1394	higher lignin degradability	1368:1394	higher lignin degradability of these fungi	1368:1409	Additionally, higher lignin degradability of these fungi can be used in biomass pretreatment, which significantly utilized to produce second-generation biofuels.					
30659858	7	15	theme	AGDR1	977:981	arg1	hardwoods					991:999	T. giganteum AGDR1 treated hardwoods	964:999	T. giganteum AGDR1 treated hardwoods	964:999	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	3	16	theme	higher	521:526	arg1	degradation					537:547	higher hardwood degradation	521:547	higher hardwood degradation	521:547	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	8	17	theme	Multivariate	1089:1100	arg1	approach					1102:1109	Multivariate approach	1089:1109	Multivariate approach	1089:1109	Multivariate approach revealed the degradation patterns and preferences are varied based on the fungi and hardwood.					
30659858	3	18	theme	fungal	501:506	arg1	isolates					508:515	twenty-one fungal isolates	490:515	twenty-one fungal isolates for higher hardwood degradation	490:547	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	6	19	theme	units	917:921	arg1	deviation					895:903	maximum deviation	887:903	maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses	887:961	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	7	20	theme	treated	983:989	arg1	hardwoods					991:999	T. giganteum AGDR1 treated hardwoods	964:999	T. giganteum AGDR1 treated hardwoods	964:999	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	1	21	theme	sensitive	169:177	arg1	techniques					179:188	fast, validate and sensitive techniques	150:188	fast, validate and sensitive techniques	150:188	Evaluating the biomass degradation using fast, validate and sensitive techniques for exploratory purposes of biofuel production has been developed since last decade.					
30659858	3	22	theme	Pseudolagarobasidium	400:419	arg1	fungi					386:390	Two white rot fungi	372:390	Two white rot fungi	372:390	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	3	22	theme	Pseudolagarobasidium	400:419	arg1	AGST3					432:436	Pseudolagarobasidium acaciicola AGST3	400:436	Pseudolagarobasidium acaciicola AGST3	400:436	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	6	23	theme	guaiacyl	908:915	arg1	units					917:921	guaiacyl units	908:921	guaiacyl units of lignin, cellulose and hemicelluloses	908:961	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	3	24	dep	Pseudolagarobasidium	400:419	arg1	acaciicola					421:430	acaciicola	421:430	acaciicola	421:430	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	7	25	theme	T.	964:965	arg1	hardwoods					991:999	T. giganteum AGDR1 treated hardwoods	964:999	T. giganteum AGDR1 treated hardwoods	964:999	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	10	26	theme	second-generation	1488:1504	arg1	biofuels					1506:1513	second-generation biofuels	1488:1513	second-generation biofuels	1488:1513	Additionally, higher lignin degradability of these fungi can be used in biomass pretreatment, which significantly utilized to produce second-generation biofuels.					
30659858	5	27	theme	cellulose	807:815	arg1	efficiency					829:838	the higher cellulose degradation efficiency	796:838	the higher cellulose degradation efficiency	796:838	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	4	28	dep	P.	568:569	arg1	acaciicola					571:580	acaciicola	571:580	acaciicola	571:580	In the screening, P. acaciicola AGST3 and T. giganteum AGDR1 depicted the dry woody mass loss of 20.51% and 22.38%, respectively.					
30659858	8	29	dep	fungi	1185:1189	arg1	the					1181:1183	the	1181:1183	the	1181:1183	Multivariate approach revealed the degradation patterns and preferences are varied based on the fungi and hardwood.					
30659858	2	30	theme	Indian	316:321	arg1	hardwoods					323:331	two Indian hardwoods	312:331	two Indian hardwoods	312:331	Thus, we assessed the degradation of two Indian hardwoods using FTIR and chemometric approaches.					
30659858	5	31	theme	degradation	817:827	arg1	efficiency					829:838	the higher cellulose degradation efficiency	796:838	the higher cellulose degradation efficiency	796:838	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	9	32	theme	present	1230:1236	arg1	study					1238:1242	the present study	1226:1242	the present study	1226:1242	The approach used in the present study can certainly distinguish the variations among the different biomass samples that having similar composition.					
30659858	9	33	theme	biomass	1305:1311	arg1	samples					1313:1319	the different biomass samples	1291:1319	the different biomass samples	1291:1319	The approach used in the present study can certainly distinguish the variations among the different biomass samples that having similar composition.					
30659858	1	34	theme	exploratory	194:204	arg1	purposes					206:213	exploratory purposes	194:213	exploratory purposes of biofuel production	194:235	Evaluating the biomass degradation using fast, validate and sensitive techniques for exploratory purposes of biofuel production has been developed since last decade.					
30659858	0	35	theme	rot	20:22	arg1	fungus					24:29	white rot fungus	14:29	white rot fungus	14:29	Assessment of white rot fungus mediated hardwood degradation by FTIR spectroscopy and multivariate analysis.					
30659858	4	36	theme	P.	568:569	arg1	AGST3					582:586	P. acaciicola AGST3	568:586	P. acaciicola AGST3	568:586	In the screening, P. acaciicola AGST3 and T. giganteum AGDR1 depicted the dry woody mass loss of 20.51% and 22.38%, respectively.					
30659858	10	37	theme	lignin	1375:1380	arg1	degradability					1382:1394	higher lignin degradability	1368:1394	higher lignin degradability of these fungi	1368:1409	Additionally, higher lignin degradability of these fungi can be used in biomass pretreatment, which significantly utilized to produce second-generation biofuels.					
30659858	3	38	theme	rot	382:384	arg1	fungi					386:390	Two white rot fungi	372:390	Two white rot fungi	372:390	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	3	38	theme	rot	382:384	arg1	AGDR1					463:467	Tricholoma giganteum AGDR1	442:467	Tricholoma giganteum AGDR1	442:467	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	3	38	theme	rot	382:384	arg1	AGST3					432:436	Pseudolagarobasidium acaciicola AGST3	400:436	Pseudolagarobasidium acaciicola AGST3	400:436	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	5	39	theme	P.	707:708	arg1	hardwoods					735:743	P. acaciicola AGST3 treated hardwoods	707:743	P. acaciicola AGST3 treated hardwoods	707:743	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	9	40	theme	different	1295:1303	arg1	samples					1313:1319	the different biomass samples	1291:1319	the different biomass samples	1291:1319	The approach used in the present study can certainly distinguish the variations among the different biomass samples that having similar composition.					
30659858	2	41	theme	chemometric	348:358	arg1	approaches					360:369	FTIR and chemometric approaches	339:369	FTIR and chemometric approaches	339:369	Thus, we assessed the degradation of two Indian hardwoods using FTIR and chemometric approaches.					
30659858	10	42	theme	higher	1368:1373	arg1	degradability					1382:1394	higher lignin degradability	1368:1394	higher lignin degradability of these fungi	1368:1409	Additionally, higher lignin degradability of these fungi can be used in biomass pretreatment, which significantly utilized to produce second-generation biofuels.					
30659858	2	43	theme	FTIR	339:342	arg1	approaches					360:369	FTIR and chemometric approaches	339:369	FTIR and chemometric approaches	339:369	Thus, we assessed the degradation of two Indian hardwoods using FTIR and chemometric approaches.					
30659858	4	44	theme	%	652:652	arg1	loss					639:642	the dry woody mass loss	620:642	the dry woody mass loss of 20.51% and 22.38%, respectively	620:677	In the screening, P. acaciicola AGST3 and T. giganteum AGDR1 depicted the dry woody mass loss of 20.51% and 22.38%, respectively.					
30659858	3	45	theme	Tricholoma	442:451	arg1	fungi					386:390	Two white rot fungi	372:390	Two white rot fungi	372:390	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	3	45	theme	Tricholoma	442:451	arg1	AGDR1					463:467	Tricholoma giganteum AGDR1	442:467	Tricholoma giganteum AGDR1	442:467	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	1	46	theme	biofuel	218:224	arg1	production					226:235	biofuel production	218:235	biofuel production	218:235	Evaluating the biomass degradation using fast, validate and sensitive techniques for exploratory purposes of biofuel production has been developed since last decade.					
30659858	0	47	theme	fungus	24:29	arg1	Assessment					0:9	Assessment	0:9	Assessment of white rot fungus	0:29	Assessment of white rot fungus mediated hardwood degradation by FTIR spectroscopy and multivariate analysis.					
30659858	5	48	theme	AGST3	721:725	arg1	hardwoods					735:743	P. acaciicola AGST3 treated hardwoods	707:743	P. acaciicola AGST3 treated hardwoods	707:743	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	5	49	theme	hardwoods	735:743	arg1	crystallinity					690:702	Cellulose crystallinity	680:702	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods	680:743	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	3	50	theme	white	376:380	arg1	fungi					386:390	Two white rot fungi	372:390	Two white rot fungi	372:390	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	3	50	theme	white	376:380	arg1	AGDR1					463:467	Tricholoma giganteum AGDR1	442:467	Tricholoma giganteum AGDR1	442:467	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	3	50	theme	white	376:380	arg1	AGST3					432:436	Pseudolagarobasidium acaciicola AGST3	400:436	Pseudolagarobasidium acaciicola AGST3	400:436	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	7	51	theme	units	1053:1057	arg1	deviation					1016:1024	maximum deviation	1008:1024	maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses	1008:1086	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	1	52	theme	production	226:235	arg1	purposes					206:213	exploratory purposes	194:213	exploratory purposes of biofuel production	194:235	Evaluating the biomass degradation using fast, validate and sensitive techniques for exploratory purposes of biofuel production has been developed since last decade.					
30659858	5	53	theme	higher	800:805	arg1	efficiency					829:838	the higher cellulose degradation efficiency	796:838	the higher cellulose degradation efficiency	796:838	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	3	54	theme	giganteum	453:461	arg1	fungi					386:390	Two white rot fungi	372:390	Two white rot fungi	372:390	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	3	54	theme	giganteum	453:461	arg1	AGDR1					463:467	Tricholoma giganteum AGDR1	442:467	Tricholoma giganteum AGDR1	442:467	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	7	55	theme	lignin	1062:1067	arg1	units					1053:1057	guaiacyl- and syringyl- units	1029:1057	guaiacyl- and syringyl- units of lignin and hemicelluloses	1029:1086	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	0	56	theme	hardwood	40:47	arg1	degradation					49:59	hardwood degradation	40:59	hardwood degradation	40:59	Assessment of white rot fungus mediated hardwood degradation by FTIR spectroscopy and multivariate analysis.					
30659858	10	57	used	utilized	1468:1475	arg2	pretreatment					1434:1445	biomass pretreatment	1426:1445	biomass pretreatment	1426:1445	Additionally, higher lignin degradability of these fungi can be used in biomass pretreatment, which significantly utilized to produce second-generation biofuels.					
30659858	6	58	theme	treated	861:867	arg1	samples					869:875	P. acaciicola AGST3 treated samples	841:875	P. acaciicola AGST3 treated samples	841:875	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	6	59	theme	AGST3	855:859	arg1	samples					869:875	P. acaciicola AGST3 treated samples	841:875	P. acaciicola AGST3 treated samples	841:875	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	1	60	theme	biomass	124:130	arg1	degradation					132:142	the biomass degradation	120:142	the biomass degradation using fast, validate and sensitive techniques	120:188	Evaluating the biomass degradation using fast, validate and sensitive techniques for exploratory purposes of biofuel production has been developed since last decade.					
30659858	4	61	dep	T.	592:593	arg1	giganteum					595:603	giganteum	595:603	giganteum	595:603	In the screening, P. acaciicola AGST3 and T. giganteum AGDR1 depicted the dry woody mass loss of 20.51% and 22.38%, respectively.					
30659858	6	62	theme	hemicelluloses	948:961	arg1	units					917:921	guaiacyl units	908:921	guaiacyl units of lignin, cellulose and hemicelluloses	908:961	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	5	63	theme	Cellulose	680:688	arg1	crystallinity					690:702	Cellulose crystallinity	680:702	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods	680:743	Cellulose crystallinity of P. acaciicola AGST3 treated hardwoods was 4-fold lower than untreated hardwoods, showing the higher cellulose degradation efficiency.					
30659858	4	64	theme	%	663:663	arg1	loss					639:642	the dry woody mass loss	620:642	the dry woody mass loss of 20.51% and 22.38%, respectively	620:677	In the screening, P. acaciicola AGST3 and T. giganteum AGDR1 depicted the dry woody mass loss of 20.51% and 22.38%, respectively.					
30659858	7	65	theme	syringyl-	1043:1051	arg1	units					1053:1057	guaiacyl- and syringyl- units	1029:1057	guaiacyl- and syringyl- units of lignin and hemicelluloses	1029:1086	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	3	66	theme	twenty-one	490:499	arg1	isolates					508:515	twenty-one fungal isolates	490:515	twenty-one fungal isolates for higher hardwood degradation	490:547	Two white rot fungi, namely Pseudolagarobasidium acaciicola AGST3 and Tricholoma giganteum AGDR1, were selected among twenty-one fungal isolates for higher hardwood degradation.					
30659858	10	67	used	used	1418:1421	arg2	degradability					1382:1394	higher lignin degradability	1368:1394	higher lignin degradability of these fungi	1368:1409	Additionally, higher lignin degradability of these fungi can be used in biomass pretreatment, which significantly utilized to produce second-generation biofuels.					
30659858	4	68	theme	dry	624:626	arg1	loss					639:642	the dry woody mass loss	620:642	the dry woody mass loss of 20.51% and 22.38%, respectively	620:677	In the screening, P. acaciicola AGST3 and T. giganteum AGDR1 depicted the dry woody mass loss of 20.51% and 22.38%, respectively.					
30659858	10	69	theme	biomass	1426:1432	arg1	pretreatment					1434:1445	biomass pretreatment	1426:1445	biomass pretreatment	1426:1445	Additionally, higher lignin degradability of these fungi can be used in biomass pretreatment, which significantly utilized to produce second-generation biofuels.					
30659858	2	70	theme	hardwoods	323:331	arg1	degradation					297:307	the degradation	293:307	the degradation of two Indian hardwoods	293:331	Thus, we assessed the degradation of two Indian hardwoods using FTIR and chemometric approaches.					
30659858	0	71	theme	FTIR	64:67	arg1	spectroscopy					69:80	FTIR spectroscopy	64:80	FTIR spectroscopy	64:80	Assessment of white rot fungus mediated hardwood degradation by FTIR spectroscopy and multivariate analysis.					
30659858	6	72	theme	P.	841:842	arg1	samples					869:875	P. acaciicola AGST3 treated samples	841:875	P. acaciicola AGST3 treated samples	841:875	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	7	73	theme	guaiacyl-	1029:1037	arg1	units					1053:1057	guaiacyl- and syringyl- units	1029:1057	guaiacyl- and syringyl- units of lignin and hemicelluloses	1029:1086	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	1	74	theme	fast	150:153	arg1	techniques					179:188	fast, validate and sensitive techniques	150:188	fast, validate and sensitive techniques	150:188	Evaluating the biomass degradation using fast, validate and sensitive techniques for exploratory purposes of biofuel production has been developed since last decade.					
30659858	4	75	theme	mass	634:637	arg1	loss					639:642	the dry woody mass loss	620:642	the dry woody mass loss of 20.51% and 22.38%, respectively	620:677	In the screening, P. acaciicola AGST3 and T. giganteum AGDR1 depicted the dry woody mass loss of 20.51% and 22.38%, respectively.					
30659858	6	76	dep	P.	841:842	arg1	acaciicola					844:853	acaciicola	844:853	acaciicola	844:853	P. acaciicola AGST3 treated samples exhibited maximum deviation of guaiacyl units of lignin, cellulose and hemicelluloses.					
30659858	9	77	theme	similar	1333:1339	arg1	composition					1341:1351	similar composition	1333:1351	similar composition	1333:1351	The approach used in the present study can certainly distinguish the variations among the different biomass samples that having similar composition.					
30659858	0	78	theme	white	14:18	arg1	fungus					24:29	white rot fungus	14:29	white rot fungus	14:29	Assessment of white rot fungus mediated hardwood degradation by FTIR spectroscopy and multivariate analysis.					
30659858	7	79	theme	maximum	1008:1014	arg1	deviation					1016:1024	maximum deviation	1008:1024	maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses	1008:1086	T. giganteum AGDR1 treated hardwoods showed maximum deviation of guaiacyl- and syringyl- units of lignin and hemicelluloses.					
30659858	4	80	theme	woody	628:632	arg1	loss					639:642	the dry woody mass loss	620:642	the dry woody mass loss of 20.51% and 22.38%, respectively	620:677	In the screening, P. acaciicola AGST3 and T. giganteum AGDR1 depicted the dry woody mass loss of 20.51% and 22.38%, respectively.					
31726160	0	0	with	film	39:42	arg1	tea					49:51	tea	49:51	tea	49:51	Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method.					
31726160	3	1	dep	Fourier	556:562	arg1	transform					564:572	transform	564:572	transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	564:638	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	9	2	theme	TP	1467:1468	arg1	incorporation					1440:1452	the simultaneous incorporation	1423:1452	the simultaneous incorporation of AgNPs and TP	1423:1468	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	0	3	theme	silver	90:95	arg1	nanoparticle					97:108	green synthesis silver nanoparticle	74:108	green synthesis silver nanoparticle	74:108	Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method.					
31726160	7	4	theme	denser	1127:1132	arg1	cross-section					1134:1146	The denser cross-section	1123:1146	The denser cross-section of CS/TP-AgNPs nanocomposite film	1123:1180	The denser cross-section of CS/TP-AgNPs nanocomposite film confirmed the enhanced mechanical properties.					
31726160	9	5	theme	TP	1507:1508	arg1	effects					1496:1502	the multiple effects	1483:1502	the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film	1483:1597	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	3	6	theme	field	502:506	arg1	FE-SEM					547:552	FE-SEM	547:552	FE-SEM	547:552	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	6	theme	field	502:506	arg1	Fourier					556:562	Fourier	556:562	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	556:638	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	6	theme	field	502:506	arg1	microscopy					535:544	field emission scanning electron microscopy	502:544	field emission scanning electron microscopy (FE-SEM)	502:553	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	6	7	theme	vapor	985:989	arg1	permeability					991:1002	water vapor permeability	979:1002	water vapor permeability of CS/TP-AgNPs nanocomposite film	979:1036	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	3	8	theme	emission	508:515	arg1	FE-SEM					547:552	FE-SEM	547:552	FE-SEM	547:552	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	8	theme	emission	508:515	arg1	Fourier					556:562	Fourier	556:562	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	556:638	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	8	theme	emission	508:515	arg1	microscopy					535:544	field emission scanning electron microscopy	502:544	field emission scanning electron microscopy (FE-SEM)	502:553	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	4	9	theme	films	738:742	arg1	properties					706:715	the physical, mechanical, antioxidant and antibacterial properties	650:715	the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films	650:742	Besides, the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films were also analyzed.					
31726160	6	10	theme	water	979:983	arg1	permeability					991:1002	water vapor permeability	979:1002	water vapor permeability of CS/TP-AgNPs nanocomposite film	979:1036	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	4	11	theme	antioxidant	676:686	arg1	properties					706:715	the physical, mechanical, antioxidant and antibacterial properties	650:715	the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films	650:742	Besides, the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films were also analyzed.					
31726160	1	12	theme	novel	289:293	arg1	method					303:308	a novel one-pot method	287:308	a novel one-pot method	287:308	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	0	13	theme	one-pot	122:128	arg1	method					130:135	a novel one-pot method	114:135	a novel one-pot method	114:135	Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method.					
31726160	9	14	theme	AgNPs	1457:1461	arg1	incorporation					1440:1452	the simultaneous incorporation	1423:1452	the simultaneous incorporation of AgNPs and TP	1423:1468	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	3	15	theme	nanocomposite	450:462	arg1	films					464:468	The AgNPs and nanocomposite films	436:468	films	464:468	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	16	theme	electron	526:533	arg1	FE-SEM					547:552	FE-SEM	547:552	FE-SEM	547:552	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	16	theme	electron	526:533	arg1	Fourier					556:562	Fourier	556:562	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	556:638	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	16	theme	electron	526:533	arg1	microscopy					535:544	field emission scanning electron microscopy	502:544	field emission scanning electron microscopy (FE-SEM)	502:553	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	4	17	theme	physical	654:661	arg1	properties					706:715	the physical, mechanical, antioxidant and antibacterial properties	650:715	the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films	650:742	Besides, the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films were also analyzed.					
31726160	1	18	theme	antioxidant	164:174	arg1	nanoparticles					226:238	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	0	19	theme	novel	116:120	arg1	method					130:135	a novel one-pot method	114:135	a novel one-pot method	114:135	Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method.					
31726160	7	20	theme	enhanced	1196:1203	arg1	properties					1216:1225	the enhanced mechanical properties	1192:1225	the enhanced mechanical properties	1192:1225	The denser cross-section of CS/TP-AgNPs nanocomposite film confirmed the enhanced mechanical properties.					
31726160	2	21	theme	AgNPs	377:381	arg1	agent					368:372	the reducing agent	355:372	the reducing agent of AgNPs	355:381	The TP was added to the CS film not only as the reducing agent of AgNPs but also as the cross-linking agent and antioxidant.					
31726160	2	21	theme	AgNPs	377:381	arg1	TP					315:316	The TP	311:316	The TP	311:316	The TP was added to the CS film not only as the reducing agent of AgNPs but also as the cross-linking agent and antioxidant.					
31726160	5	22	theme	incorporation	791:803	arg1	content					771:777	the content	767:777	the content of TP-AgNPs incorporation	767:803	As the content of TP-AgNPs incorporation increased, the color of CS/TP-AgNPs nanocomposite film gradually shifted to brown, accompanied by an increase in opacity and thickness.					
31726160	6	23	from	permeability	991:1002	arg1	contents					966:973	moisture contents	957:973	moisture contents	957:973	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	4	24	theme	nanocomposite	724:736	arg1	films					738:742	the nanocomposite films	720:742	the nanocomposite films	720:742	Besides, the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films were also analyzed.					
31726160	1	25	theme	antibacterial	180:192	arg1	nanoparticles					226:238	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	0	26	theme	Antioxidant	0:10	arg1	film					39:42	Antioxidant and antibacterial chitosan film	0:42	film	39:42	Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method.					
31726160	1	27	theme	one-pot	295:301	arg1	method					303:308	a novel one-pot method	287:308	a novel one-pot method	287:308	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	8	28	theme	nanocomposite	1264:1276	arg1	film					1278:1281	CS/TP-AgNPs nanocomposite film	1252:1281	CS/TP-AgNPs nanocomposite film	1252:1281	It is worth noting that CS/TP-AgNPs nanocomposite film exhibited more excellent antioxidant and antibacterial activity than CS film.					
31726160	5	29	theme	film	855:858	arg1	color					820:824	the color	816:824	the color of CS/TP-AgNPs nanocomposite film	816:858	As the content of TP-AgNPs incorporation increased, the color of CS/TP-AgNPs nanocomposite film gradually shifted to brown, accompanied by an increase in opacity and thickness.					
31726160	1	30	theme	chitosan/tea	194:205	arg1	nanoparticles					226:238	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	0	31	theme	chitosan	30:37	arg1	film					39:42	Antioxidant and antibacterial chitosan film	0:42	film	39:42	Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method.					
31726160	6	32	theme	moisture	957:964	arg1	contents					966:973	moisture contents	957:973	moisture contents	957:973	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	5	33	from	increase	906:913	arg1	thickness					930:938	thickness	930:938	thickness	930:938	As the content of TP-AgNPs incorporation increased, the color of CS/TP-AgNPs nanocomposite film gradually shifted to brown, accompanied by an increase in opacity and thickness.					
31726160	5	33	from	increase	906:913	arg1	opacity					918:924	opacity	918:924	opacity	918:924	As the content of TP-AgNPs incorporation increased, the color of CS/TP-AgNPs nanocomposite film gradually shifted to brown, accompanied by an increase in opacity and thickness.					
31726160	2	34	theme	cross-linking	399:411	arg1	agent					413:417	the cross-linking agent	395:417	the cross-linking agent	395:417	The TP was added to the CS film not only as the reducing agent of AgNPs but also as the cross-linking agent and antioxidant.					
31726160	2	34	theme	cross-linking	399:411	arg1	TP					315:316	The TP	311:316	The TP	311:316	The TP was added to the CS film not only as the reducing agent of AgNPs but also as the cross-linking agent and antioxidant.					
31726160	0	35	theme	antibacterial	16:28	arg1	film					39:42	Antioxidant and antibacterial chitosan film	0:42	film	39:42	Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method.					
31726160	9	36	theme	multi-functionality	1386:1404	arg1	film					1415:1418	the multi-functionality chitosan film	1382:1418	the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP	1382:1468	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	6	37	from	decrease	945:952	arg1	contents					966:973	moisture contents	957:973	moisture contents	957:973	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	3	38	dep	transform	564:572	arg1	infrared					574:581	infrared	574:581	transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	564:638	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	1	39	theme	present	145:151	arg1	study					153:157	the present study	141:157	the present study	141:157	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	7	40	theme	CS/TP-AgNPs	1151:1161	arg1	film					1177:1180	CS/TP-AgNPs nanocomposite film	1151:1180	CS/TP-AgNPs nanocomposite film	1151:1180	The denser cross-section of CS/TP-AgNPs nanocomposite film confirmed the enhanced mechanical properties.					
31726160	9	41	theme	chitosan	1406:1413	arg1	film					1415:1418	the multi-functionality chitosan film	1382:1418	the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP	1382:1468	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	5	42	theme	CS/TP-AgNPs	829:839	arg1	film					855:858	CS/TP-AgNPs nanocomposite film	829:858	CS/TP-AgNPs nanocomposite film	829:858	As the content of TP-AgNPs incorporation increased, the color of CS/TP-AgNPs nanocomposite film gradually shifted to brown, accompanied by an increase in opacity and thickness.					
31726160	5	43	dep	shifted	870:876	arg1	accompanied					888:898	accompanied	888:898	accompanied by an increase in opacity and thickness	888:938	As the content of TP-AgNPs incorporation increased, the color of CS/TP-AgNPs nanocomposite film gradually shifted to brown, accompanied by an increase in opacity and thickness.					
31726160	8	44	theme	CS	1352:1353	arg1	film					1355:1358	CS film	1352:1358	CS film	1352:1358	It is worth noting that CS/TP-AgNPs nanocomposite film exhibited more excellent antioxidant and antibacterial activity than CS film.					
31726160	1	45	theme	composite	240:248	arg1	CS/TP-AgNPs					256:266	CS/TP-AgNPs	256:266	CS/TP-AgNPs	256:266	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	1	45	theme	composite	240:248	arg1	film					250:253	composite film	240:253	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	4	46	theme	mechanical	664:673	arg1	properties					706:715	the physical, mechanical, antioxidant and antibacterial properties	650:715	the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films	650:742	Besides, the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films were also analyzed.					
31726160	7	47	theme	nanocomposite	1163:1175	arg1	film					1177:1180	CS/TP-AgNPs nanocomposite film	1151:1180	CS/TP-AgNPs nanocomposite film	1151:1180	The denser cross-section of CS/TP-AgNPs nanocomposite film confirmed the enhanced mechanical properties.					
31726160	7	48	theme	film	1177:1180	arg1	cross-section					1134:1146	The denser cross-section	1123:1146	The denser cross-section of CS/TP-AgNPs nanocomposite film	1123:1180	The denser cross-section of CS/TP-AgNPs nanocomposite film confirmed the enhanced mechanical properties.					
31726160	3	49	theme	AgNPs	440:444	arg1	films					464:468	The AgNPs and nanocomposite films	436:468	films	464:468	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	8	50	theme	excellent	1298:1306	arg1	antioxidant					1308:1318	more excellent antioxidant	1293:1318	more excellent antioxidant	1293:1318	It is worth noting that CS/TP-AgNPs nanocomposite film exhibited more excellent antioxidant and antibacterial activity than CS film.					
31726160	9	51	theme	reducing	1520:1527	arg1	agent					1529:1533	reducing agent	1520:1533	reducing agent for AgNPs	1520:1543	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	9	52	theme	multiple	1487:1494	arg1	effects					1496:1502	the multiple effects	1483:1502	the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film	1483:1597	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	4	53	theme	antibacterial	692:704	arg1	properties					706:715	the physical, mechanical, antioxidant and antibacterial properties	650:715	the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films	650:742	Besides, the physical, mechanical, antioxidant and antibacterial properties of the nanocomposite films were also analyzed.					
31726160	5	54	theme	TP-AgNPs	782:789	arg1	incorporation					791:803	TP-AgNPs incorporation	782:803	TP-AgNPs incorporation	782:803	As the content of TP-AgNPs incorporation increased, the color of CS/TP-AgNPs nanocomposite film gradually shifted to brown, accompanied by an increase in opacity and thickness.					
31726160	6	55	dep	CS-CS	1103:1107	arg1	the					1099:1101	the	1099:1101	the	1099:1101	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	9	56	from	effects	1496:1502	arg1	chitosan					1585:1592	chitosan film	1585:1597	chitosan film	1585:1597	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	8	57	theme	antibacterial	1324:1336	arg1	activity					1338:1345	antibacterial activity	1324:1345	antibacterial activity	1324:1345	It is worth noting that CS/TP-AgNPs nanocomposite film exhibited more excellent antioxidant and antibacterial activity than CS film.					
31726160	9	58	theme	simultaneous	1427:1438	arg1	incorporation					1440:1452	the simultaneous incorporation	1423:1452	the simultaneous incorporation of AgNPs and TP	1423:1468	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	3	59	theme	thermogravimetric	607:623	arg1	TGA					635:637	TGA	635:637	TGA	635:637	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	59	theme	thermogravimetric	607:623	arg1	analysis					625:632	thermogravimetric analysis	607:632	thermogravimetric analysis (TGA)	607:638	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	2	60	theme	CS	335:336	arg1	film					338:341	the CS film	331:341	the CS film	331:341	The TP was added to the CS film not only as the reducing agent of AgNPs but also as the cross-linking agent and antioxidant.					
31726160	6	61	theme	film	1033:1036	arg1	permeability					991:1002	water vapor permeability	979:1002	water vapor permeability of CS/TP-AgNPs nanocomposite film	979:1036	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	6	61	theme	film	1033:1036	arg1	decrease					945:952	The decrease	941:952	The decrease in moisture contents	941:973	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	8	62	theme	CS/TP-AgNPs	1252:1262	arg1	film					1278:1281	CS/TP-AgNPs nanocomposite film	1252:1281	CS/TP-AgNPs nanocomposite film	1252:1281	It is worth noting that CS/TP-AgNPs nanocomposite film exhibited more excellent antioxidant and antibacterial activity than CS film.					
31726160	9	63	theme	cross-linking	1546:1558	arg1	agent					1560:1564	cross-linking agent	1546:1564	cross-linking agent	1546:1564	This study developed the multi-functionality chitosan film by the simultaneous incorporation of AgNPs and TP and revealed the multiple effects of TP acting as reducing agent for AgNPs, cross-linking agent and antioxidant on chitosan film.					
31726160	6	64	theme	nanocomposite	1019:1031	arg1	film					1033:1036	CS/TP-AgNPs nanocomposite film	1007:1036	CS/TP-AgNPs nanocomposite film	1007:1036	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	0	65	theme	synthesis	80:88	arg1	nanoparticle					97:108	green synthesis silver nanoparticle	74:108	green synthesis silver nanoparticle	74:108	Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method.					
31726160	5	66	theme	nanocomposite	841:853	arg1	film					855:858	CS/TP-AgNPs nanocomposite film	829:858	CS/TP-AgNPs nanocomposite film	829:858	As the content of TP-AgNPs incorporation increased, the color of CS/TP-AgNPs nanocomposite film gradually shifted to brown, accompanied by an increase in opacity and thickness.					
31726160	1	67	theme	polyphenols-silver	207:224	arg1	nanoparticles					226:238	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	2	68	theme	reducing	359:366	arg1	agent					368:372	the reducing agent	355:372	the reducing agent of AgNPs	355:381	The TP was added to the CS film not only as the reducing agent of AgNPs but also as the cross-linking agent and antioxidant.					
31726160	2	68	theme	reducing	359:366	arg1	TP					315:316	The TP	311:316	The TP	311:316	The TP was added to the CS film not only as the reducing agent of AgNPs but also as the cross-linking agent and antioxidant.					
31726160	6	69	theme	original	1077:1084	arg1	bond					1086:1089	the original bond	1073:1089	the original bond between the CS-CS and CS-water	1073:1120	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	6	70	theme	CS/TP-AgNPs	1007:1017	arg1	film					1033:1036	CS/TP-AgNPs nanocomposite film	1007:1036	CS/TP-AgNPs nanocomposite film	1007:1036	The decrease in moisture contents and water vapor permeability of CS/TP-AgNPs nanocomposite film indicated that the TP-AgNPs varied the original bond between the CS-CS and CS-water.					
31726160	0	71	theme	green	74:78	arg1	nanoparticle					97:108	green synthesis silver nanoparticle	74:108	green synthesis silver nanoparticle	74:108	Antioxidant and antibacterial chitosan film with tea polyphenols-mediated green synthesis silver nanoparticle via a novel one-pot method.					
31726160	7	72	theme	mechanical	1205:1214	arg1	properties					1216:1225	the enhanced mechanical properties	1192:1225	the enhanced mechanical properties	1192:1225	The denser cross-section of CS/TP-AgNPs nanocomposite film confirmed the enhanced mechanical properties.					
31726160	1	73	dep	nanoparticles	226:238	arg1	CS/TP-AgNPs					256:266	CS/TP-AgNPs	256:266	CS/TP-AgNPs	256:266	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	1	73	dep	nanoparticles	226:238	arg1	film					250:253	composite film	240:253	the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs)	160:267	In the present study, the antioxidant and antibacterial chitosan/tea polyphenols-silver nanoparticles composite film (CS/TP-AgNPs) was developed via a novel one-pot method.					
31726160	3	74	theme	scanning	517:524	arg1	FE-SEM					547:552	FE-SEM	547:552	FE-SEM	547:552	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	74	theme	scanning	517:524	arg1	Fourier					556:562	Fourier	556:562	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	556:638	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
31726160	3	74	theme	scanning	517:524	arg1	microscopy					535:544	field emission scanning electron microscopy	502:544	field emission scanning electron microscopy (FE-SEM)	502:553	The AgNPs and nanocomposite films developed were characterized by field emission scanning electron microscopy (FE-SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).					
30252451	0	0	theme	Bone	91:94	arg1	Matrices					96:103	Biomimetic Bone Matrices	80:103	Biomimetic Bone Matrices	80:103	Influence of Regioselectively Sulfated Cellulose on in Vitro Vascularization of Biomimetic Bone Matrices.					
30252451	6	1	from	impact	922:927	arg1	function					944:951	endothelial function	932:951	endothelial function	932:951	Their impact on endothelial function may possibly be due to interactions with cell surface receptors/soluble factors not yet defined.					
30252451	4	2	theme	-induced	732:739	arg1	formation					748:756	vascular endothelial growth factor (VEGF)-induced tubule formation	691:756	vascular endothelial growth factor (VEGF)-induced tubule formation	691:756	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	1	3	theme	tissue	164:169	arg1	regeneration					143:154	the regeneration	139:154	the regeneration of bone tissue within composite material	139:195	Vascularization is essential for the regeneration of bone tissue within composite material.					
30252451	4	4	theme	polysaccharide	621:634	arg1	backbone					636:643	the polysaccharide backbone	617:643	the polysaccharide backbone (13-TACS-01)	617:656	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	4	4	theme	polysaccharide	621:634	arg1	13-TACS-01					646:655	13-TACS-01	646:655	13-TACS-01	646:655	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	0	5	theme	Biomimetic	80:89	arg1	Matrices					96:103	Biomimetic Bone Matrices	80:103	Biomimetic Bone Matrices	80:103	Influence of Regioselectively Sulfated Cellulose on in Vitro Vascularization of Biomimetic Bone Matrices.					
30252451	2	6	theme	cells	405:409	arg1	formation					365:373	the tubule formation	354:373	the tubule formation of human vascular endothelial cells	354:409	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	4	7	with	sulfate	570:576	arg1	pattern					603:609	irregular sulfation pattern	583:609	irregular sulfation pattern along the polysaccharide backbone (13-TACS-01)	583:656	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	0	8	from	Influence	0:8	arg1	Vascularization					61:75	in Vitro Vascularization	52:75	in Vitro Vascularization of Biomimetic Bone Matrices	52:103	Influence of Regioselectively Sulfated Cellulose on in Vitro Vascularization of Biomimetic Bone Matrices.					
30252451	2	9	theme	nanocomposite	337:349	arg1	scaffolds					300:308	porous scaffolds	293:308	porous scaffolds of collagen/hydroxyapatite nanocomposite	293:349	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	4	10	theme	endothelial	700:710	arg1	VEGF					727:730	VEGF	727:730	VEGF	727:730	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	4	10	theme	endothelial	700:710	arg1	factor					719:724	vascular endothelial growth factor	691:724	vascular endothelial growth factor (VEGF)	691:731	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	0	11	theme	Matrices	96:103	arg1	Vascularization					61:75	in Vitro Vascularization	52:75	in Vitro Vascularization of Biomimetic Bone Matrices	52:103	Influence of Regioselectively Sulfated Cellulose on in Vitro Vascularization of Biomimetic Bone Matrices.					
30252451	2	12	theme	collagen/hydroxyapatite	313:335	arg1	nanocomposite					337:349	collagen/hydroxyapatite nanocomposite	313:349	collagen/hydroxyapatite nanocomposite	313:349	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	5	13	theme	apparent	889:896	arg1	affinity					898:905	no apparent affinity	886:905	no apparent affinity to VEGF	886:913	In contrast with structurally different heparin, these cellulose sulfates have no apparent affinity to VEGF.					
30252451	4	14	dep	in	777:778	arg1	vitro					780:784	vitro	780:784	vitro	780:784	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	5	15	theme	different	837:845	arg1	heparin					847:853	structurally different heparin	824:853	structurally different heparin	824:853	In contrast with structurally different heparin, these cellulose sulfates have no apparent affinity to VEGF.					
30252451	1	16	theme	composite	178:186	arg1	material					188:195	composite material	178:195	composite material	178:195	Vascularization is essential for the regeneration of bone tissue within composite material.					
30252451	2	17	from	effect	214:219	arg1	formation					365:373	the tubule formation	354:373	the tubule formation of human vascular endothelial cells	354:409	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	5	18	with	contrast	810:817	arg1	heparin					847:853	structurally different heparin	824:853	structurally different heparin	824:853	In contrast with structurally different heparin, these cellulose sulfates have no apparent affinity to VEGF.					
30252451	4	19	theme	vascular	691:698	arg1	VEGF					727:730	VEGF	727:730	VEGF	727:730	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	4	19	theme	vascular	691:698	arg1	factor					719:724	vascular endothelial growth factor	691:724	vascular endothelial growth factor (VEGF)	691:731	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	3	20	theme	endothelial	433:443	arg1	cells					445:449	endothelial cells	433:449	endothelial cells	433:449	Using a coculture of endothelial cells and fibroblasts, endothelial cells formed a network of tubules within an incubation time of 14 to 24 days.					
30252451	5	21	contain	have	881:884	arg2	affinity					898:905	no apparent affinity	886:905	no apparent affinity to VEGF	886:913	In contrast with structurally different heparin, these cellulose sulfates have no apparent affinity to VEGF.					
30252451	5	21	contain	have	881:884	arg1	sulfates					872:879	these cellulose sulfates	856:879	these cellulose sulfates	856:879	In contrast with structurally different heparin, these cellulose sulfates have no apparent affinity to VEGF.					
30252451	3	22	theme	fibroblasts	455:465	arg1	coculture					420:428	a coculture	418:428	a coculture of endothelial cells and fibroblasts	418:465	Using a coculture of endothelial cells and fibroblasts, endothelial cells formed a network of tubules within an incubation time of 14 to 24 days.					
30252451	3	23	theme	cells	445:449	arg1	coculture					420:428	a coculture	418:428	a coculture of endothelial cells and fibroblasts	418:465	Using a coculture of endothelial cells and fibroblasts, endothelial cells formed a network of tubules within an incubation time of 14 to 24 days.					
30252451	4	24	theme	sulfation	593:601	arg1	pattern					603:609	irregular sulfation pattern	583:609	irregular sulfation pattern along the polysaccharide backbone (13-TACS-01)	583:656	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	2	25	theme	tubule	358:363	arg1	formation					365:373	the tubule formation	354:373	the tubule formation of human vascular endothelial cells	354:409	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	4	26	theme	growth	712:717	arg1	VEGF					727:730	VEGF	727:730	VEGF	727:730	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	4	26	theme	growth	712:717	arg1	factor					719:724	vascular endothelial growth factor	691:724	vascular endothelial growth factor (VEGF)	691:731	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	0	27	theme	Sulfated	30:37	arg1	Cellulose					39:47	Regioselectively Sulfated Cellulose	13:47	Regioselectively Sulfated Cellulose	13:47	Influence of Regioselectively Sulfated Cellulose on in Vitro Vascularization of Biomimetic Bone Matrices.					
30252451	4	28	theme	irregular	583:591	arg1	pattern					603:609	irregular sulfation pattern	583:609	irregular sulfation pattern along the polysaccharide backbone (13-TACS-01)	583:656	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	4	29	theme	in	777:778	arg1	assays					799:804	an in vitro angiogenesis assays	774:804	an in vitro angiogenesis assays	774:804	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	4	30	from	increase	679:686	arg1	formation					748:756	vascular endothelial growth factor (VEGF)-induced tubule formation	691:756	vascular endothelial growth factor (VEGF)-induced tubule formation	691:756	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	4	31	theme	additional	668:677	arg1	increase					679:686	an additional increase	665:686	an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation	665:756	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	3	32	dep	24	549:550	arg1	to					546:547	to	546:547	to	546:547	Using a coculture of endothelial cells and fibroblasts, endothelial cells formed a network of tubules within an incubation time of 14 to 24 days.					
30252451	6	33	theme	cell	994:997	arg1	factors					1025:1031	cell surface receptors/soluble factors	994:1031	cell surface receptors/soluble factors not yet defined	994:1047	Their impact on endothelial function may possibly be due to interactions with cell surface receptors/soluble factors not yet defined.					
30252451	4	34	theme	tubule	741:746	arg1	formation					748:756	vascular endothelial growth factor (VEGF)-induced tubule formation	691:756	vascular endothelial growth factor (VEGF)-induced tubule formation	691:756	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	0	35	theme	Cellulose	39:47	arg1	Influence					0:8	Influence	0:8	Influence of Regioselectively Sulfated Cellulose on in Vitro Vascularization of Biomimetic Bone Matrices.	0:104	Influence of Regioselectively Sulfated Cellulose on in Vitro Vascularization of Biomimetic Bone Matrices.					
30252451	4	36	theme	cellulose	560:568	arg1	sulfate					570:576	A cellulose sulfate	558:576	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01)	558:656	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	3	37	theme	endothelial	468:478	arg1	cells					480:484	endothelial cells	468:484	endothelial cells	468:484	Using a coculture of endothelial cells and fibroblasts, endothelial cells formed a network of tubules within an incubation time of 14 to 24 days.					
30252451	0	38	dep	in	52:53	arg1	Vitro					55:59	Vitro	55:59	Vitro	55:59	Influence of Regioselectively Sulfated Cellulose on in Vitro Vascularization of Biomimetic Bone Matrices.					
30252451	3	39	theme	tubules	506:512	arg1	network					495:501	a network	493:501	a network of tubules	493:512	Using a coculture of endothelial cells and fibroblasts, endothelial cells formed a network of tubules within an incubation time of 14 to 24 days.					
30252451	6	40	theme	surface	999:1005	arg1	factors					1025:1031	cell surface receptors/soluble factors	994:1031	cell surface receptors/soluble factors not yet defined	994:1047	Their impact on endothelial function may possibly be due to interactions with cell surface receptors/soluble factors not yet defined.					
30252451	2	41	theme	modified	241:248	arg1	cellulose/hemicellulose					250:272	regioselectively modified cellulose/hemicellulose	224:272	regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite	224:349	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	2	42	theme	endothelial	393:403	arg1	cells					405:409	human vascular endothelial cells	378:409	human vascular endothelial cells	378:409	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	0	43	theme	in	52:53	arg1	Vascularization					61:75	in Vitro Vascularization	52:75	in Vitro Vascularization of Biomimetic Bone Matrices	52:103	Influence of Regioselectively Sulfated Cellulose on in Vitro Vascularization of Biomimetic Bone Matrices.					
30252451	2	44	theme	vascular	384:391	arg1	cells					405:409	human vascular endothelial cells	378:409	human vascular endothelial cells	378:409	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	2	45	theme	porous	293:298	arg1	scaffolds					300:308	porous scaffolds	293:308	porous scaffolds of collagen/hydroxyapatite nanocomposite	293:349	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	2	46	theme	cellulose/hemicellulose	250:272	arg1	effect					214:219	the effect	210:219	the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells	210:409	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	2	47	theme	human	378:382	arg1	cells					405:409	human vascular endothelial cells	378:409	human vascular endothelial cells	378:409	We measured the effect of regioselectively modified cellulose/hemicellulose as an additive for porous scaffolds of collagen/hydroxyapatite nanocomposite on the tubule formation of human vascular endothelial cells.					
30252451	6	48	with	interactions	976:987	arg1	factors					1025:1031	cell surface receptors/soluble factors	994:1031	cell surface receptors/soluble factors not yet defined	994:1047	Their impact on endothelial function may possibly be due to interactions with cell surface receptors/soluble factors not yet defined.					
30252451	4	49	theme	angiogenesis	786:797	arg1	assays					799:804	an in vitro angiogenesis assays	774:804	an in vitro angiogenesis assays	774:804	A cellulose sulfate with irregular sulfation pattern along the polysaccharide backbone (13-TACS-01) led to an additional increase in vascular endothelial growth factor (VEGF)-induced tubule formation, as observed in an in vitro angiogenesis assays.					
30252451	3	50	theme	incubation	524:533	arg1	time					535:538	an incubation time	521:538	an incubation time of 14 to 24 days	521:555	Using a coculture of endothelial cells and fibroblasts, endothelial cells formed a network of tubules within an incubation time of 14 to 24 days.					
30252451	5	51	theme	cellulose	862:870	arg1	sulfates					872:879	these cellulose sulfates	856:879	these cellulose sulfates	856:879	In contrast with structurally different heparin, these cellulose sulfates have no apparent affinity to VEGF.					
30252451	3	52	theme	days	552:555	arg1	time					535:538	an incubation time	521:538	an incubation time of 14 to 24 days	521:555	Using a coculture of endothelial cells and fibroblasts, endothelial cells formed a network of tubules within an incubation time of 14 to 24 days.					
30252451	6	53	theme	endothelial	932:942	arg1	function					944:951	endothelial function	932:951	endothelial function	932:951	Their impact on endothelial function may possibly be due to interactions with cell surface receptors/soluble factors not yet defined.					
30252451	6	54	theme	receptors/soluble	1007:1023	arg1	factors					1025:1031	cell surface receptors/soluble factors	994:1031	cell surface receptors/soluble factors not yet defined	994:1047	Their impact on endothelial function may possibly be due to interactions with cell surface receptors/soluble factors not yet defined.					
30252451	1	55	theme	bone	159:162	arg1	tissue					164:169	bone tissue	159:169	bone tissue	159:169	Vascularization is essential for the regeneration of bone tissue within composite material.					
30398401	13	0	theme	SDS-treated	2035:2045	arg1	hydrogels					2047:2055	the SDS-treated hydrogels	2031:2055	the SDS-treated hydrogels	2031:2055	This effect was more pronounced in the SDS-treated hydrogels when compared with the Triton groups, showing very little retention of other ECM molecules.					
30398401	6	1	theme	dodecyl	1115:1121	arg1	sulfate					1123:1129	sodium dodecyl sulfate [SDS	1108:1134	sodium dodecyl sulfate [SDS	1108:1134	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	6	1	theme	dodecyl	1115:1121	arg1	detergents					1096:1105	two different detergents	1082:1105	two different detergents (sodium dodecyl sulfate [SDS]	1082:1135	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	2	2	theme	tissue	362:367	arg1	composition					336:346	the biochemical composition	320:346	the biochemical composition of the native tissue	320:367	Ideally, the proper scaffold should mimic the biochemical composition of the native tissue as it drives numerous signaling pathways involved in tissue homeostasis and functionality.					
30398401	13	3	theme	other	2128:2132	arg1	molecules					2138:2146	other ECM molecules	2128:2146	other ECM molecules	2128:2146	This effect was more pronounced in the SDS-treated hydrogels when compared with the Triton groups, showing very little retention of other ECM molecules.					
30398401	12	4	theme	fresh	1980:1984	arg1	pancreas					1986:1993	fresh pancreas	1980:1993	fresh pancreas	1980:1993	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	14	5	theme	matricellular	2183:2195	arg1	proteins					2197:2204	matricellular proteins	2183:2204	matricellular proteins	2183:2204	Conversely, basement membrane and matricellular proteins were better retained when the tissue was pretreated with a PI and decellularized in Triton X-100, making the hydrogel more similar to the native tissue.					
30398401	2	6	theme	proper	291:296	arg1	scaffold					298:305	the proper scaffold	287:305	the proper scaffold	287:305	Ideally, the proper scaffold should mimic the biochemical composition of the native tissue as it drives numerous signaling pathways involved in tissue homeostasis and functionality.					
30398401	15	7	theme	hydrogel	2598:2605	arg1	closer					2611:2616	closer	2611:2616	closer	2611:2616	In conclusion, we showed that all the protocols evaluated in the study showed effective tissue decellularization, but only when the tissue was pretreated with a PI and decellularized in Triton detergent, the biochemical composition of the hydrogel was closer to the native tissue ECM.					
30398401	15	7	theme	hydrogel	2598:2605	arg1	composition					2579:2589	the biochemical composition	2563:2589	the biochemical composition of the hydrogel	2563:2605	In conclusion, we showed that all the protocols evaluated in the study showed effective tissue decellularization, but only when the tissue was pretreated with a PI and decellularized in Triton detergent, the biochemical composition of the hydrogel was closer to the native tissue ECM.					
30398401	13	8	theme	molecules	2138:2146	arg1	retention					2115:2123	very little retention	2103:2123	very little retention of other ECM molecules	2103:2146	This effect was more pronounced in the SDS-treated hydrogels when compared with the Triton groups, showing very little retention of other ECM molecules.					
30398401	16	9	theme	Impact	2644:2649	arg1	Statement					2651:2659	Impact Statement The article	2644:2671	Impact Statement The article	2644:2671	Impact Statement The article compares different methodologies for the generation of a pancreas-derived hydrogel for tissue engineering applications.					
30398401	3	10	theme	ideal	570:574	arg1	scaffold					576:583	an ideal scaffold	567:583	an ideal scaffold for 3D tissue engineering applications	567:622	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	12	11	theme	higher	1952:1957	arg1	amount					1959:1964	a higher amount	1950:1964	a higher amount compared with fresh pancreas	1950:1993	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	3	12	link	Tissue-derived	460:473	arg1	biomaterials					490:501	Tissue-derived decellularized biomaterials	460:501	Tissue-derived decellularized biomaterials	460:501	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	14	13	theme	similar	2329:2335	arg1	hydrogel					2315:2322	the hydrogel	2311:2322	the hydrogel more similar to the native tissue	2311:2356	Conversely, basement membrane and matricellular proteins were better retained when the tissue was pretreated with a PI and decellularized in Triton X-100, making the hydrogel more similar to the native tissue.					
30398401	15	14	theme	biochemical	2567:2577	arg1	closer					2611:2616	closer	2611:2616	closer	2611:2616	In conclusion, we showed that all the protocols evaluated in the study showed effective tissue decellularization, but only when the tissue was pretreated with a PI and decellularized in Triton detergent, the biochemical composition of the hydrogel was closer to the native tissue ECM.					
30398401	15	14	theme	biochemical	2567:2577	arg1	composition					2579:2589	the biochemical composition	2563:2589	the biochemical composition of the hydrogel	2563:2605	In conclusion, we showed that all the protocols evaluated in the study showed effective tissue decellularization, but only when the tissue was pretreated with a PI and decellularized in Triton detergent, the biochemical composition of the hydrogel was closer to the native tissue ECM.					
30398401	9	15	theme	hematoxylin	1437:1447	arg1	staining					1465:1472	hematoxylin and eosin (H&E) staining	1437:1472	hematoxylin and eosin (H&E) staining	1437:1472	Effective decellularization was confirmed by PicoGreen assay and hematoxylin and eosin (H&E) staining, showing no differences among groups.					
30398401	10	16	theme	firm	1555:1558	arg1	hydrogel					1560:1567	a firm hydrogel	1553:1567	a firm hydrogel	1553:1567	Triton-treated samples were able to form a firm hydrogel under appropriate conditions, while the use of SDS had detrimental effects on the gelation properties of the hydrogels.					
30398401	10	17	theme	hydrogels	1678:1686	arg1	properties					1660:1669	the gelation properties	1647:1669	the gelation properties of the hydrogels	1647:1686	Triton-treated samples were able to form a firm hydrogel under appropriate conditions, while the use of SDS had detrimental effects on the gelation properties of the hydrogels.					
30398401	9	18	theme	eosin	1453:1457	arg1	staining					1465:1472	hematoxylin and eosin (H&E) staining	1437:1472	hematoxylin and eosin (H&E) staining	1437:1472	Effective decellularization was confirmed by PicoGreen assay and hematoxylin and eosin (H&E) staining, showing no differences among groups.					
30398401	12	19	theme	generated	1923:1931	arg1	hydrogels					1933:1941	all generated hydrogels	1919:1941	all generated hydrogels	1919:1941	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	4	20	theme	specific	692:699	arg1	components					701:710	specific components	692:710	specific components	692:710	However, the decellularization process may affect the retention of specific components, and the choice of a proper detergent is fundamental in preserving the native ECM composition.					
30398401	13	21	theme	little	2108:2113	arg1	retention					2115:2123	very little retention	2103:2123	very little retention of other ECM molecules	2103:2146	This effect was more pronounced in the SDS-treated hydrogels when compared with the Triton groups, showing very little retention of other ECM molecules.					
30398401	15	22	theme	tissue	2447:2452	arg1	decellularization					2454:2470	effective tissue decellularization	2437:2470	effective tissue decellularization	2437:2470	In conclusion, we showed that all the protocols evaluated in the study showed effective tissue decellularization, but only when the tissue was pretreated with a PI and decellularized in Triton detergent, the biochemical composition of the hydrogel was closer to the native tissue ECM.					
30398401	16	23	dep	Statement	2651:2659	arg1	article					2665:2671	The article	2661:2671	Impact Statement The article	2644:2671	Impact Statement The article compares different methodologies for the generation of a pancreas-derived hydrogel for tissue engineering applications.					
30398401	6	24	theme	porcine	986:992	arg1	tissue					1003:1008	Fresh porcine pancreas tissue	980:1008	Fresh porcine pancreas tissue	980:1008	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	16	25	theme	pancreas-derived	2730:2745	arg1	hydrogel					2747:2754	a pancreas-derived hydrogel	2728:2754	a pancreas-derived hydrogel for tissue engineering applications	2728:2790	Impact Statement The article compares different methodologies for the generation of a pancreas-derived hydrogel for tissue engineering applications.					
30398401	11	26	theme	pECM	1793:1796	arg1	hydrogels					1798:1806	all decellularized pECM hydrogels	1774:1806	all decellularized pECM hydrogels	1774:1806	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	5	27	theme	pECM	963:966	arg1	hydrogels					969:977	pancreatic ECM (pECM) hydrogels	947:977	pancreatic ECM (pECM) hydrogels	947:977	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	5	28	theme	hydrogels	969:977	arg1	properties					905:914	mechanical properties	894:914	mechanical properties	894:914	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	5	28	theme	hydrogels	969:977	arg1	composition					932:942	biochemical composition	920:942	biochemical composition	920:942	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	16	29	theme	engineering	2767:2777	arg1	applications					2779:2790	tissue engineering applications	2760:2790	tissue engineering applications	2760:2790	Impact Statement The article compares different methodologies for the generation of a pancreas-derived hydrogel for tissue engineering applications.					
30398401	11	30	dep	pancreas	1761:1768	arg1	both					1735:1738	both	1735:1738	both	1735:1738	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	5	31	theme	different	849:857	arg1	protocols					877:885	different decellularization protocols	849:885	different decellularization protocols	849:885	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	1	32	theme	matrices	179:186	arg1	effects					144:150	the effects	140:150	the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function	140:246	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	4	33	theme	proper	733:738	arg1	detergent					740:748	a proper detergent	731:748	a proper detergent	731:748	However, the decellularization process may affect the retention of specific components, and the choice of a proper detergent is fundamental in preserving the native ECM composition.					
30398401	14	34	theme	Triton	2290:2295	arg1	X-100					2297:2301	Triton X-100	2290:2301	Triton X-100	2290:2301	Conversely, basement membrane and matricellular proteins were better retained when the tissue was pretreated with a PI and decellularized in Triton X-100, making the hydrogel more similar to the native tissue.					
30398401	11	35	theme	fresh	1747:1751	arg1	pancreas					1761:1768	the fresh porcine pancreas	1743:1768	the fresh porcine pancreas	1743:1768	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	0	36	theme	Hydrogels	90:98	arg1	Generation					59:68	the Generation	55:68	the Generation of Pancreas-Derived Hydrogels	55:98	Evaluation of Different Decellularization Protocols on the Generation of Pancreas-Derived Hydrogels.					
30398401	5	37	theme	ECM	958:960	arg1	hydrogels					969:977	pancreatic ECM (pECM) hydrogels	947:977	pancreatic ECM (pECM) hydrogels	947:977	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	6	38	theme	small	1034:1038	arg1	pieces					1040:1045	small pieces	1034:1045	small pieces	1034:1045	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	0	39	from	Evaluation	0:9	arg1	Generation					59:68	the Generation	55:68	the Generation of Pancreas-Derived Hydrogels	55:98	Evaluation of Different Decellularization Protocols on the Generation of Pancreas-Derived Hydrogels.					
30398401	7	40	theme	Effective	1193:1201	arg1	decellularization					1203:1219	Effective decellularization	1193:1219	Effective decellularization	1193:1219	Effective decellularization was confirmed by PicoGreen assay, Hoescht, and H&E staining, showing no differences among groups.					
30398401	1	41	theme	different	155:163	arg1	matrices					179:186	different extracellular matrices	155:186	different extracellular matrices (ECMs)	155:193	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	1	41	theme	different	155:163	arg1	ECMs					189:192	ECMs	189:192	ECMs	189:192	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	8	42	theme	inhibitor	1337:1345	arg1	Use					1319:1321	Use	1319:1321	Use of a protease inhibitor (PI)	1319:1350	Use of a protease inhibitor (PI) was also evaluated.					
30398401	17	43	theme	extracellular	2898:2910	arg1	molecules					2912:2920	all the extracellular molecules	2890:2920	all the extracellular molecules of the native tissue	2890:2941	The biochemical characterization of the newly generated hydrogel shows that the material retains all the extracellular molecules of the native tissue and is capable of sustaining functionality of the encapsulated beta-cells.					
30398401	3	44	theme	Tissue-derived	460:473	arg1	biomaterials					490:501	Tissue-derived decellularized biomaterials	460:501	Tissue-derived decellularized biomaterials	460:501	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	12	45	theme	major	1879:1883	arg1	component					1889:1897	the major ECM component	1875:1897	the major ECM component in all groups	1875:1911	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	12	45	theme	major	1879:1883	arg1	collagen					1862:1869	Fibrillar collagen	1852:1869	Fibrillar collagen	1852:1869	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	2	46	theme	signaling	391:399	arg1	pathways					401:408	numerous signaling pathways	382:408	numerous signaling pathways involved in tissue homeostasis and functionality	382:457	Ideally, the proper scaffold should mimic the biochemical composition of the native tissue as it drives numerous signaling pathways involved in tissue homeostasis and functionality.					
30398401	4	47	theme	ECM	790:792	arg1	composition					794:804	the native ECM composition	779:804	the native ECM composition	779:804	However, the decellularization process may affect the retention of specific components, and the choice of a proper detergent is fundamental in preserving the native ECM composition.					
30398401	0	48	theme	Different	14:22	arg1	Protocols					42:50	Different Decellularization Protocols	14:50	Different Decellularization Protocols	14:50	Evaluation of Different Decellularization Protocols on the Generation of Pancreas-Derived Hydrogels.					
30398401	12	49	theme	Fibrillar	1852:1860	arg1	component					1889:1897	the major ECM component	1875:1897	the major ECM component in all groups	1875:1911	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	12	49	theme	Fibrillar	1852:1860	arg1	collagen					1862:1869	Fibrillar collagen	1852:1869	Fibrillar collagen	1852:1869	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	16	50	theme	different	2682:2690	arg1	methodologies					2692:2704	different methodologies	2682:2704	different methodologies for the generation of a pancreas-derived hydrogel for tissue engineering applications	2682:2790	Impact Statement The article compares different methodologies for the generation of a pancreas-derived hydrogel for tissue engineering applications.					
30398401	17	51	theme	tissue	2936:2941	arg1	molecules					2912:2920	all the extracellular molecules	2890:2920	all the extracellular molecules of the native tissue	2890:2941	The biochemical characterization of the newly generated hydrogel shows that the material retains all the extracellular molecules of the native tissue and is capable of sustaining functionality of the encapsulated beta-cells.					
30398401	1	52	dep	three-dimensional	199:215	arg1	3D					218:219	3D	218:219	3D	218:219	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	2	53	theme	tissue	422:427	arg1	homeostasis					429:439	tissue homeostasis	422:439	tissue homeostasis	422:439	Ideally, the proper scaffold should mimic the biochemical composition of the native tissue as it drives numerous signaling pathways involved in tissue homeostasis and functionality.					
30398401	9	54	theme	PicoGreen	1417:1425	arg1	assay					1427:1431	PicoGreen assay	1417:1431	PicoGreen assay	1417:1431	Effective decellularization was confirmed by PicoGreen assay and hematoxylin and eosin (H&E) staining, showing no differences among groups.					
30398401	0	55	theme	Protocols	42:50	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Different Decellularization Protocols on the Generation of Pancreas-Derived Hydrogels.	0:99	Evaluation of Different Decellularization Protocols on the Generation of Pancreas-Derived Hydrogels.					
30398401	2	56	theme	native	355:360	arg1	tissue					362:367	the native tissue	351:367	the native tissue	351:367	Ideally, the proper scaffold should mimic the biochemical composition of the native tissue as it drives numerous signaling pathways involved in tissue homeostasis and functionality.					
30398401	6	57	theme	Triton	1140:1145	arg1	X-100					1147:1151	Triton X-100	1140:1151	Triton X-100	1140:1151	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	17	58	theme	encapsulated	2993:3004	arg1	beta-cells					3006:3015	the encapsulated beta-cells	2989:3015	the encapsulated beta-cells	2989:3015	The biochemical characterization of the newly generated hydrogel shows that the material retains all the extracellular molecules of the native tissue and is capable of sustaining functionality of the encapsulated beta-cells.					
30398401	11	59	theme	biochemical	1693:1703	arg1	composition					1705:1715	ECM biochemical composition	1689:1715	ECM biochemical composition	1689:1715	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	11	60	theme	mass	1824:1827	arg1	analysis					1842:1849	quantitative mass spectrometry analysis	1811:1849	quantitative mass spectrometry analysis	1811:1849	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	1	61	theme	islet	233:237	arg1	function					239:246	islet function	233:246	islet function	233:246	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	4	62	theme	decellularization	638:654	arg1	process					656:662	the decellularization process	634:662	the decellularization process	634:662	However, the decellularization process may affect the retention of specific components, and the choice of a proper detergent is fundamental in preserving the native ECM composition.					
30398401	14	63	theme	basement	2161:2168	arg1	membrane					2170:2177	basement membrane	2161:2177	basement membrane	2161:2177	Conversely, basement membrane and matricellular proteins were better retained when the tissue was pretreated with a PI and decellularized in Triton X-100, making the hydrogel more similar to the native tissue.					
30398401	10	64	theme	Triton-treated	1512:1525	arg1	samples					1527:1533	Triton-treated samples	1512:1533	Triton-treated samples	1512:1533	Triton-treated samples were able to form a firm hydrogel under appropriate conditions, while the use of SDS had detrimental effects on the gelation properties of the hydrogels.					
30398401	3	65	theme	native	543:548	arg1	tissue					550:555	the native tissue	539:555	the native tissue	539:555	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	1	66	theme	encouraging	257:267	arg1	results					269:275	encouraging results	257:275	encouraging results	257:275	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	6	67	theme	sodium	1108:1113	arg1	sulfate					1123:1129	sodium dodecyl sulfate [SDS	1108:1134	sodium dodecyl sulfate [SDS	1108:1134	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	6	67	theme	sodium	1108:1113	arg1	detergents					1096:1105	two different detergents	1082:1105	two different detergents (sodium dodecyl sulfate [SDS]	1082:1135	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	17	68	theme	beta-cells	3006:3015	arg1	functionality					2972:2984	functionality	2972:2984	functionality of the encapsulated beta-cells	2972:3015	The biochemical characterization of the newly generated hydrogel shows that the material retains all the extracellular molecules of the native tissue and is capable of sustaining functionality of the encapsulated beta-cells.					
30398401	13	69	theme	ECM	2134:2136	arg1	molecules					2138:2146	other ECM molecules	2128:2146	other ECM molecules	2128:2146	This effect was more pronounced in the SDS-treated hydrogels when compared with the Triton groups, showing very little retention of other ECM molecules.					
30398401	3	70	theme	engineering	599:609	arg1	applications					611:622	3D tissue engineering applications	589:622	3D tissue engineering applications	589:622	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	10	71	contain	had	1620:1622	arg1	use					1609:1611	the use	1605:1611	the use of SDS	1605:1618	Triton-treated samples were able to form a firm hydrogel under appropriate conditions, while the use of SDS had detrimental effects on the gelation properties of the hydrogels.					
30398401	10	71	contain	had	1620:1622	arg2	effects					1636:1642	detrimental effects	1624:1642	detrimental effects	1624:1642	Triton-treated samples were able to form a firm hydrogel under appropriate conditions, while the use of SDS had detrimental effects on the gelation properties of the hydrogels.					
30398401	17	72	theme	generated	2839:2847	arg1	hydrogel					2849:2856	the newly generated hydrogel	2829:2856	the newly generated hydrogel	2829:2856	The biochemical characterization of the newly generated hydrogel shows that the material retains all the extracellular molecules of the native tissue and is capable of sustaining functionality of the encapsulated beta-cells.					
30398401	14	73	theme	native	2344:2349	arg1	tissue					2351:2356	the native tissue	2340:2356	the native tissue	2340:2356	Conversely, basement membrane and matricellular proteins were better retained when the tissue was pretreated with a PI and decellularized in Triton X-100, making the hydrogel more similar to the native tissue.					
30398401	5	74	theme	mechanical	894:903	arg1	properties					905:914	mechanical properties	894:914	mechanical properties	894:914	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	4	75	theme	components	701:710	arg1	retention					679:687	the retention	675:687	the retention of specific components	675:710	However, the decellularization process may affect the retention of specific components, and the choice of a proper detergent is fundamental in preserving the native ECM composition.					
30398401	10	76	theme	appropriate	1575:1585	arg1	conditions					1587:1596	appropriate conditions	1575:1596	appropriate conditions	1575:1596	Triton-treated samples were able to form a firm hydrogel under appropriate conditions, while the use of SDS had detrimental effects on the gelation properties of the hydrogels.					
30398401	2	77	theme	biochemical	324:334	arg1	composition					336:346	the biochemical composition	320:346	the biochemical composition of the native tissue	320:367	Ideally, the proper scaffold should mimic the biochemical composition of the native tissue as it drives numerous signaling pathways involved in tissue homeostasis and functionality.					
30398401	1	78	theme	three-dimensional	199:215	arg1	culture					222:228	three-dimensional (3D) culture	199:228	three-dimensional (3D) culture	199:228	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	13	79	from	pronounced	2017:2026	arg1	hydrogels					2047:2055	the SDS-treated hydrogels	2031:2055	the SDS-treated hydrogels	2031:2055	This effect was more pronounced in the SDS-treated hydrogels when compared with the Triton groups, showing very little retention of other ECM molecules.					
30398401	11	80	theme	decellularized	1778:1791	arg1	hydrogels					1798:1806	all decellularized pECM hydrogels	1774:1806	all decellularized pECM hydrogels	1774:1806	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	3	81	theme	3D	589:590	arg1	applications					611:622	3D tissue engineering applications	589:622	3D tissue engineering applications	589:622	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	5	82	theme	decellularization	859:875	arg1	protocols					877:885	different decellularization protocols	849:885	different decellularization protocols	849:885	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	5	83	theme	biochemical	920:930	arg1	composition					932:942	biochemical composition	920:942	biochemical composition	920:942	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	1	84	theme	culture	222:228	arg1	effects					144:150	the effects	140:150	the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function	140:246	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	6	85	theme	pancreas	994:1001	arg1	tissue					1003:1008	Fresh porcine pancreas tissue	980:1008	Fresh porcine pancreas tissue	980:1008	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	12	86	from	component	1889:1897	arg1	groups					1906:1911	all groups	1902:1911	all groups	1902:1911	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	13	87	from	hydrogels	2047:2055	arg1	pronounced					2017:2026	pronounced	2017:2026	pronounced	2017:2026	This effect was more pronounced in the SDS-treated hydrogels when compared with the Triton groups, showing very little retention of other ECM molecules.					
30398401	11	88	theme	quantitative	1811:1822	arg1	analysis					1842:1849	quantitative mass spectrometry analysis	1811:1849	quantitative mass spectrometry analysis	1811:1849	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	6	89	theme	Fresh	980:984	arg1	tissue					1003:1008	Fresh porcine pancreas tissue	980:1008	Fresh porcine pancreas tissue	980:1008	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	4	90	theme	detergent	740:748	arg1	choice					721:726	the choice	717:726	the choice of a proper detergent	717:748	However, the decellularization process may affect the retention of specific components, and the choice of a proper detergent is fundamental in preserving the native ECM composition.					
30398401	4	90	theme	detergent	740:748	arg1	fundamental					753:763	fundamental	753:763	fundamental	753:763	However, the decellularization process may affect the retention of specific components, and the choice of a proper detergent is fundamental in preserving the native ECM composition.					
30398401	13	91	theme	Triton	2080:2085	arg1	groups					2087:2092	the Triton groups	2076:2092	the Triton groups	2076:2092	This effect was more pronounced in the SDS-treated hydrogels when compared with the Triton groups, showing very little retention of other ECM molecules.					
30398401	15	92	theme	effective	2437:2445	arg1	decellularization					2454:2470	effective tissue decellularization	2437:2470	effective tissue decellularization	2437:2470	In conclusion, we showed that all the protocols evaluated in the study showed effective tissue decellularization, but only when the tissue was pretreated with a PI and decellularized in Triton detergent, the biochemical composition of the hydrogel was closer to the native tissue ECM.					
30398401	16	93	link	pancreas-derived	2730:2745	arg1	hydrogel					2747:2754	a pancreas-derived hydrogel	2728:2754	a pancreas-derived hydrogel for tissue engineering applications	2728:2790	Impact Statement The article compares different methodologies for the generation of a pancreas-derived hydrogel for tissue engineering applications.					
30398401	16	94	theme	hydrogel	2747:2754	arg1	generation					2714:2723	the generation	2710:2723	the generation of a pancreas-derived hydrogel for tissue engineering applications	2710:2790	Impact Statement The article compares different methodologies for the generation of a pancreas-derived hydrogel for tissue engineering applications.					
30398401	3	95	theme	ECM	520:522	arg1	composition					524:534	the ECM composition	516:534	the ECM composition of the native tissue	516:555	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	0	96	theme	Pancreas-Derived	73:88	arg1	Hydrogels					90:98	Pancreas-Derived Hydrogels	73:98	Pancreas-Derived Hydrogels	73:98	Evaluation of Different Decellularization Protocols on the Generation of Pancreas-Derived Hydrogels.					
30398401	1	97	theme	extracellular	165:177	arg1	matrices					179:186	different extracellular matrices	155:186	different extracellular matrices (ECMs)	155:193	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	1	97	theme	extracellular	165:177	arg1	ECMs					189:192	ECMs	189:192	ECMs	189:192	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	16	98	theme	tissue	2760:2765	arg1	applications					2779:2790	tissue engineering applications	2760:2790	tissue engineering applications	2760:2790	Impact Statement The article compares different methodologies for the generation of a pancreas-derived hydrogel for tissue engineering applications.					
30398401	15	99	theme	native	2625:2630	arg1	ECM					2639:2641	the native tissue ECM	2621:2641	the native tissue ECM	2621:2641	In conclusion, we showed that all the protocols evaluated in the study showed effective tissue decellularization, but only when the tissue was pretreated with a PI and decellularized in Triton detergent, the biochemical composition of the hydrogel was closer to the native tissue ECM.					
30398401	15	100	theme	tissue	2632:2637	arg1	ECM					2639:2641	the native tissue ECM	2621:2641	the native tissue ECM	2621:2641	In conclusion, we showed that all the protocols evaluated in the study showed effective tissue decellularization, but only when the tissue was pretreated with a PI and decellularized in Triton detergent, the biochemical composition of the hydrogel was closer to the native tissue ECM.					
30398401	5	101	theme	pancreatic	947:956	arg1	hydrogels					969:977	pancreatic ECM (pECM) hydrogels	947:977	pancreatic ECM (pECM) hydrogels	947:977	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	17	102	theme	biochemical	2797:2807	arg1	characterization					2809:2824	The biochemical characterization	2793:2824	The biochemical characterization of the newly generated hydrogel	2793:2856	The biochemical characterization of the newly generated hydrogel shows that the material retains all the extracellular molecules of the native tissue and is capable of sustaining functionality of the encapsulated beta-cells.					
30398401	11	103	theme	porcine	1753:1759	arg1	pancreas					1761:1768	the fresh porcine pancreas	1743:1768	the fresh porcine pancreas	1743:1768	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	3	104	theme	tissue	592:597	arg1	applications					611:622	3D tissue engineering applications	589:622	3D tissue engineering applications	589:622	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	10	105	theme	gelation	1651:1658	arg1	properties					1660:1669	the gelation properties	1647:1669	the gelation properties of the hydrogels	1647:1686	Triton-treated samples were able to form a firm hydrogel under appropriate conditions, while the use of SDS had detrimental effects on the gelation properties of the hydrogels.					
30398401	12	106	theme	ECM	1885:1887	arg1	component					1889:1897	the major ECM component	1875:1897	the major ECM component in all groups	1875:1911	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	12	106	theme	ECM	1885:1887	arg1	collagen					1862:1869	Fibrillar collagen	1852:1869	Fibrillar collagen	1852:1869	Fibrillar collagen was the major ECM component in all groups, with all generated hydrogels having a higher amount compared with fresh pancreas.					
30398401	5	107	theme	protocols	877:885	arg1	effect					839:844	the effect	835:844	the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels	835:977	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	8	108	theme	protease	1328:1335	arg1	PI					1348:1349	PI	1348:1349	PI	1348:1349	Use of a protease inhibitor (PI) was also evaluated.					
30398401	8	108	theme	protease	1328:1335	arg1	inhibitor					1337:1345	a protease inhibitor	1326:1345	a protease inhibitor (PI)	1326:1350	Use of a protease inhibitor (PI) was also evaluated.					
30398401	6	109	from	days	1178:1181	arg1	water					1186:1190	water	1186:1190	water	1186:1190	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	10	110	theme	SDS	1616:1618	arg1	use					1609:1611	the use	1605:1611	the use of SDS	1605:1618	Triton-treated samples were able to form a firm hydrogel under appropriate conditions, while the use of SDS had detrimental effects on the gelation properties of the hydrogels.					
30398401	5	111	from	effect	839:844	arg1	properties					905:914	mechanical properties	894:914	mechanical properties	894:914	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	5	111	from	effect	839:844	arg1	composition					932:942	biochemical composition	920:942	biochemical composition	920:942	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	1	112	theme	Different	101:109	arg1	approaches					111:120	Different approaches	101:120	Different approaches	101:120	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	6	113	theme	different	1086:1094	arg1	sulfate					1123:1129	sodium dodecyl sulfate [SDS	1108:1134	sodium dodecyl sulfate [SDS	1108:1134	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	6	113	theme	different	1086:1094	arg1	detergents					1096:1105	two different detergents	1082:1105	two different detergents (sodium dodecyl sulfate [SDS]	1082:1135	Fresh porcine pancreas tissue was harvested, cut into small pieces, rinsed in water, and treated with two different detergents (sodium dodecyl sulfate [SDS] or Triton X-100) for 1 day followed by 3 days in water.					
30398401	2	114	theme	numerous	382:389	arg1	pathways					401:408	numerous signaling pathways	382:408	numerous signaling pathways involved in tissue homeostasis and functionality	382:457	Ideally, the proper scaffold should mimic the biochemical composition of the native tissue as it drives numerous signaling pathways involved in tissue homeostasis and functionality.					
30398401	0	115	theme	Decellularization	24:40	arg1	Protocols					42:50	Different Decellularization Protocols	14:50	Different Decellularization Protocols	14:50	Evaluation of Different Decellularization Protocols on the Generation of Pancreas-Derived Hydrogels.					
30398401	1	116	from	effects	144:150	arg1	function					239:246	islet function	233:246	islet function	233:246	Different approaches have investigated the effects of different extracellular matrices (ECMs) and three-dimensional (3D) culture on islet function, showing encouraging results.					
30398401	4	117	theme	native	783:788	arg1	composition					794:804	the native ECM composition	779:804	the native ECM composition	779:804	However, the decellularization process may affect the retention of specific components, and the choice of a proper detergent is fundamental in preserving the native ECM composition.					
30398401	17	118	theme	native	2929:2934	arg1	tissue					2936:2941	the native tissue	2925:2941	the native tissue	2925:2941	The biochemical characterization of the newly generated hydrogel shows that the material retains all the extracellular molecules of the native tissue and is capable of sustaining functionality of the encapsulated beta-cells.					
30398401	10	119	theme	detrimental	1624:1634	arg1	effects					1636:1642	detrimental effects	1624:1642	detrimental effects	1624:1642	Triton-treated samples were able to form a firm hydrogel under appropriate conditions, while the use of SDS had detrimental effects on the gelation properties of the hydrogels.					
30398401	7	120	theme	H&E	1268:1270	arg1	staining					1272:1279	H&E staining	1268:1279	H&E staining	1268:1279	Effective decellularization was confirmed by PicoGreen assay, Hoescht, and H&E staining, showing no differences among groups.					
30398401	11	121	theme	ECM	1689:1691	arg1	composition					1705:1715	ECM biochemical composition	1689:1715	ECM biochemical composition	1689:1715	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	5	122	dep	properties	905:914	arg1	the					890:892	the	890:892	the	890:892	In this study, we evaluated the effect of different decellularization protocols on the mechanical properties and biochemical composition of pancreatic ECM (pECM) hydrogels.					
30398401	3	123	theme	tissue	550:555	arg1	composition					524:534	the ECM composition	516:534	the ECM composition of the native tissue	516:555	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	17	124	theme	hydrogel	2849:2856	arg1	characterization					2809:2824	The biochemical characterization	2793:2824	The biochemical characterization of the newly generated hydrogel	2793:2856	The biochemical characterization of the newly generated hydrogel shows that the material retains all the extracellular molecules of the native tissue and is capable of sustaining functionality of the encapsulated beta-cells.					
30398401	15	125	theme	Triton	2545:2550	arg1	detergent					2552:2560	Triton detergent	2545:2560	Triton detergent	2545:2560	In conclusion, we showed that all the protocols evaluated in the study showed effective tissue decellularization, but only when the tissue was pretreated with a PI and decellularized in Triton detergent, the biochemical composition of the hydrogel was closer to the native tissue ECM.					
30398401	11	126	theme	spectrometry	1829:1840	arg1	analysis					1842:1849	quantitative mass spectrometry analysis	1811:1849	quantitative mass spectrometry analysis	1811:1849	ECM biochemical composition was characterized both in the fresh porcine pancreas and all decellularized pECM hydrogels by quantitative mass spectrometry analysis.					
30398401	3	127	theme	decellularized	475:488	arg1	biomaterials					490:501	Tissue-derived decellularized biomaterials	460:501	Tissue-derived decellularized biomaterials	460:501	Tissue-derived decellularized biomaterials can preserve the ECM composition of the native tissue making it an ideal scaffold for 3D tissue engineering applications.					
30398401	14	128	with	pretreated	2247:2256	arg1	PI					2265:2266	a PI	2263:2266	a PI	2263:2266	Conversely, basement membrane and matricellular proteins were better retained when the tissue was pretreated with a PI and decellularized in Triton X-100, making the hydrogel more similar to the native tissue.					
30398401	7	129	theme	PicoGreen	1238:1246	arg1	assay					1248:1252	PicoGreen assay	1238:1252	PicoGreen assay	1238:1252	Effective decellularization was confirmed by PicoGreen assay, Hoescht, and H&E staining, showing no differences among groups.					
30398401	9	130	theme	Effective	1372:1380	arg1	decellularization					1382:1398	Effective decellularization	1372:1398	Effective decellularization	1372:1398	Effective decellularization was confirmed by PicoGreen assay and hematoxylin and eosin (H&E) staining, showing no differences among groups.					
30373002	1	0	theme	products	336:343	arg1	characteristics					298:312	characteristics	298:312	characteristics of finally formulated products	298:343	In order to assess the essential probiotic properties of a strain dedicated for administration in humans and animals, characteristics of finally formulated products, rather than the cells solely, seems to be of crucial importance.					
30373002	2	1	theme	statistical	528:538	arg1	design					553:558	a statistical experimental design	526:558	a statistical experimental design	526:558	In this study, composition of protective blends for manufacture of L. lactis probiotic powders was optimized using a statistical experimental design.					
30373002	6	2	theme	environmental	1075:1087	arg1	conditions					1089:1098	harsh environmental conditions	1069:1098	harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability	1069:1165	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	1	3	theme	strain	239:244	arg1	properties					223:232	the essential probiotic properties	199:232	the essential probiotic properties of a strain dedicated for administration in humans and animals	199:295	In order to assess the essential probiotic properties of a strain dedicated for administration in humans and animals, characteristics of finally formulated products, rather than the cells solely, seems to be of crucial importance.					
30373002	0	4	theme	probiotic	71:79	arg1	properties					81:90	probiotic properties	71:90	probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use	71:177	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	0	5	theme	use	175:177	arg1	process					126:132	A complete process	115:132	probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use	71:177	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	6	6	theme	53.9 ± 7.6	1112:1121	arg1	%					1122:1122	%	1122:1122	%	1122:1122	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	2	7	theme	lactis	481:486	arg1	powders					498:504	L. lactis probiotic powders	478:504	L. lactis probiotic powders	478:504	In this study, composition of protective blends for manufacture of L. lactis probiotic powders was optimized using a statistical experimental design.					
30373002	0	8	theme	Lactococcus	95:105	arg1	lactis					107:112	Lactococcus lactis	95:112	Lactococcus lactis	95:112	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	4	9	theme	adherence	767:775	arg1	capability					777:786	adherence capability	767:786	adherence capability	767:786	Finally, maintenance of adherence capability to human enterocyte-like cell lines, was evaluated.					
30373002	6	10	theme	higher	1143:1148	arg1	ability					1159:1165	higher adhesion ability	1143:1165	higher adhesion ability	1143:1165	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	5	11	theme	drying	943:948	arg1	techniques					950:959	both drying techniques	938:959	both drying techniques	938:959	Our data demonstrated that 10% trehalose ensures the highest viability of L. lactis bacteria upon both drying techniques (viability of 60-68%).					
30373002	2	12	theme	powders	498:504	arg1	manufacture					463:473	manufacture	463:473	manufacture of L. lactis probiotic powders	463:504	In this study, composition of protective blends for manufacture of L. lactis probiotic powders was optimized using a statistical experimental design.					
30373002	3	13	theme	simulated	704:712	arg1	stomach					714:720	simulated stomach	704:720	simulated stomach	704:720	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	6	14	theme	ability	1159:1165	arg1	conditions					1089:1098	harsh environmental conditions	1069:1098	harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability	1069:1165	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	2	15	theme	probiotic	488:496	arg1	powders					498:504	L. lactis probiotic powders	478:504	L. lactis probiotic powders	478:504	In this study, composition of protective blends for manufacture of L. lactis probiotic powders was optimized using a statistical experimental design.					
30373002	6	16	theme	%	1122:1122	arg1	rate					1133:1136	53.9 ± 7.6% survival rate	1112:1136	53.9 ± 7.6% survival rate	1112:1136	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	6	16	theme	%	1122:1122	arg1	stomach					1103:1109	stomach	1103:1109	stomach (53.9 ± 7.6% survival rate)	1103:1137	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	6	17	theme	adhesion	1150:1157	arg1	ability					1159:1165	higher adhesion ability	1143:1165	higher adhesion ability	1143:1165	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	0	18	theme	lactis	107:112	arg1	properties					81:90	probiotic properties	71:90	probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use	71:177	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	6	19	theme	epithelial	1226:1235	arg1	cells					1237:1241	100 epithelial cells	1222:1241	100 epithelial cells	1222:1241	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	5	20	theme	lactis	917:922	arg1	bacteria					924:931	L. lactis bacteria	914:931	L. lactis bacteria	914:931	Our data demonstrated that 10% trehalose ensures the highest viability of L. lactis bacteria upon both drying techniques (viability of 60-68%).					
30373002	6	21	theme	harsh	1069:1073	arg1	conditions					1089:1098	harsh environmental conditions	1069:1098	harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability	1069:1165	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	3	22	from	digestion	691:699	arg1	intestine					732:740	small intestine	726:740	small intestine	726:740	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	3	22	from	digestion	691:699	arg1	stomach					714:720	simulated stomach	704:720	simulated stomach	704:720	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	3	23	from	testing	669:675	arg1	intestine					732:740	small intestine	726:740	small intestine	726:740	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	3	23	from	testing	669:675	arg1	stomach					714:720	simulated stomach	704:720	simulated stomach	704:720	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	0	24	dep	properties	81:90	arg1	process					126:132	A complete process	115:132	probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use	71:177	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	6	25	theme	highest	1047:1053	arg1	resistance					1055:1064	the highest resistance	1043:1064	the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line	1043:1184	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	6	26	theme	cell	1176:1179	arg1	line					1181:1184	HT-29 cell line	1170:1184	HT-29 cell line	1170:1184	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	5	27	theme	bacteria	924:931	arg1	viability					901:909	the highest viability	889:909	the highest viability of L. lactis bacteria	889:931	Our data demonstrated that 10% trehalose ensures the highest viability of L. lactis bacteria upon both drying techniques (viability of 60-68%).					
30373002	5	28	theme	10	867:868	arg1	%					869:869	%	869:869	%	869:869	Our data demonstrated that 10% trehalose ensures the highest viability of L. lactis bacteria upon both drying techniques (viability of 60-68%).					
30373002	5	29	theme	%	980:980	arg1	viability					962:970	viability	962:970	viability of 60-68%	962:980	Our data demonstrated that 10% trehalose ensures the highest viability of L. lactis bacteria upon both drying techniques (viability of 60-68%).					
30373002	0	30	theme	complete	117:124	arg1	process					126:132	A complete process	115:132	probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use	71:177	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	2	31	theme	experimental	540:551	arg1	design					553:558	a statistical experimental design	526:558	a statistical experimental design	526:558	In this study, composition of protective blends for manufacture of L. lactis probiotic powders was optimized using a statistical experimental design.					
30373002	0	32	theme	preservation	10:21	arg1	Method					0:5	Method	0:5	Method of preservation and type of protective agent	0:50	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	3	33	dep	in	682:683	arg1	vitro					685:689	vitro	685:689	vitro	685:689	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	1	34	from	administration	260:273	arg1	animals					289:295	animals	289:295	animals	289:295	In order to assess the essential probiotic properties of a strain dedicated for administration in humans and animals, characteristics of finally formulated products, rather than the cells solely, seems to be of crucial importance.					
30373002	1	34	from	administration	260:273	arg1	humans					278:283	humans	278:283	humans	278:283	In order to assess the essential probiotic properties of a strain dedicated for administration in humans and animals, characteristics of finally formulated products, rather than the cells solely, seems to be of crucial importance.					
30373002	6	35	theme	survival	1124:1131	arg1	rate					1133:1136	53.9 ± 7.6% survival rate	1112:1136	53.9 ± 7.6% survival rate	1112:1136	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	6	35	theme	survival	1124:1131	arg1	stomach					1103:1109	stomach	1103:1109	stomach (53.9 ± 7.6% survival rate)	1103:1137	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	2	36	theme	blends	452:457	arg1	composition					426:436	composition	426:436	composition of protective blends for manufacture of L. lactis probiotic powders	426:504	In this study, composition of protective blends for manufacture of L. lactis probiotic powders was optimized using a statistical experimental design.					
30373002	3	37	theme	small	726:730	arg1	intestine					732:740	small intestine	726:740	small intestine	726:740	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	4	38	theme	cell	813:816	arg1	lines					818:822	human enterocyte-like cell lines	791:822	human enterocyte-like cell lines	791:822	Finally, maintenance of adherence capability to human enterocyte-like cell lines, was evaluated.					
30373002	6	39	theme	spray-dried	1017:1027	arg1	cells					1029:1033	skimmed milk-protected spray-dried cells	994:1033	skimmed milk-protected spray-dried cells	994:1033	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	0	40	theme	type	27:30	arg1	Method					0:5	Method	0:5	Method of preservation and type of protective agent	0:50	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	2	41	theme	protective	441:450	arg1	blends					452:457	protective blends	441:457	protective blends for manufacture of L. lactis probiotic powders	441:504	In this study, composition of protective blends for manufacture of L. lactis probiotic powders was optimized using a statistical experimental design.					
30373002	4	42	theme	enterocyte-like	797:811	arg1	lines					818:822	human enterocyte-like cell lines	791:822	human enterocyte-like cell lines	791:822	Finally, maintenance of adherence capability to human enterocyte-like cell lines, was evaluated.					
30373002	1	43	theme	essential	203:211	arg1	properties					223:232	the essential probiotic properties	199:232	the essential probiotic properties of a strain dedicated for administration in humans and animals	199:295	In order to assess the essential probiotic properties of a strain dedicated for administration in humans and animals, characteristics of finally formulated products, rather than the cells solely, seems to be of crucial importance.					
30373002	0	44	theme	agent	46:50	arg1	preservation					10:21	preservation	10:21	preservation	10:21	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	0	44	theme	agent	46:50	arg1	type					27:30	type	27:30	type	27:30	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	0	45	theme	preparation	147:157	arg1	manufacture					159:169	probiotic preparation manufacture	137:169	probiotic preparation manufacture	137:169	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	6	46	dep	digestion	1192:1200	arg1	cells					1212:1216	528 ± 29 cells	1203:1216	528 ± 29 cells per 100 epithelial cells	1203:1241	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	5	47	theme	highest	893:899	arg1	viability					901:909	the highest viability	889:909	the highest viability of L. lactis bacteria	889:931	Our data demonstrated that 10% trehalose ensures the highest viability of L. lactis bacteria upon both drying techniques (viability of 60-68%).					
30373002	0	48	theme	protective	35:44	arg1	agent					46:50	protective agent	35:50	protective agent	35:50	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	0	49	theme	probiotic	137:145	arg1	manufacture					159:169	probiotic preparation manufacture	137:169	probiotic preparation manufacture	137:169	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	3	50	theme	storage	661:667	arg1	testing					669:675	storage testing	661:675	storage testing	661:675	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	5	51	dep	demonstrated	849:860	arg1	viability					962:970	viability	962:970	viability of 60-68%	962:980	Our data demonstrated that 10% trehalose ensures the highest viability of L. lactis bacteria upon both drying techniques (viability of 60-68%).					
30373002	3	52	theme	in	682:683	arg1	digestion					691:699	in vitro digestion	682:699	in vitro digestion	682:699	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	6	53	theme	HT-29	1170:1174	arg1	line					1181:1184	HT-29 cell line	1170:1184	HT-29 cell line	1170:1184	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	1	54	theme	crucial	391:397	arg1	importance					399:408	crucial importance	391:408	crucial importance	391:408	In order to assess the essential probiotic properties of a strain dedicated for administration in humans and animals, characteristics of finally formulated products, rather than the cells solely, seems to be of crucial importance.					
30373002	4	55	theme	capability	777:786	arg1	maintenance					752:762	maintenance	752:762	maintenance	752:762	Finally, maintenance of adherence capability to human enterocyte-like cell lines, was evaluated.					
30373002	6	56	theme	528 ± 29	1203:1210	arg1	cells					1212:1216	528 ± 29 cells	1203:1216	528 ± 29 cells per 100 epithelial cells	1203:1241	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	1	57	theme	probiotic	213:221	arg1	properties					223:232	the essential probiotic properties	199:232	the essential probiotic properties of a strain dedicated for administration in humans and animals	199:295	In order to assess the essential probiotic properties of a strain dedicated for administration in humans and animals, characteristics of finally formulated products, rather than the cells solely, seems to be of crucial importance.					
30373002	0	58	theme	manufacture	159:169	arg1	process					126:132	A complete process	115:132	probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use	71:177	Method of preservation and type of protective agent strongly influence probiotic properties of Lactococcus lactis: A complete process of probiotic preparation manufacture and use.					
30373002	3	59	theme	freeze-drying	604:616	arg1	techniques					618:627	either spray- or freeze-drying techniques	587:627	either spray- or freeze-drying techniques	587:627	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	6	60	theme	stomach	1103:1109	arg1	conditions					1089:1098	harsh environmental conditions	1069:1098	harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability	1069:1165	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	2	61	theme	L.	478:479	arg1	powders					498:504	L. lactis probiotic powders	478:504	L. lactis probiotic powders	478:504	In this study, composition of protective blends for manufacture of L. lactis probiotic powders was optimized using a statistical experimental design.					
30373002	6	62	theme	milk-protected	1002:1015	arg1	cells					1029:1033	skimmed milk-protected spray-dried cells	994:1033	skimmed milk-protected spray-dried cells	994:1033	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30373002	4	63	theme	human	791:795	arg1	lines					818:822	human enterocyte-like cell lines	791:822	human enterocyte-like cell lines	791:822	Finally, maintenance of adherence capability to human enterocyte-like cell lines, was evaluated.					
30373002	5	64	theme	%	869:869	arg1	trehalose					871:879	10% trehalose	867:879	10% trehalose	867:879	Our data demonstrated that 10% trehalose ensures the highest viability of L. lactis bacteria upon both drying techniques (viability of 60-68%).					
30373002	1	65	theme	formulated	325:334	arg1	products					336:343	finally formulated products	317:343	finally formulated products	317:343	In order to assess the essential probiotic properties of a strain dedicated for administration in humans and animals, characteristics of finally formulated products, rather than the cells solely, seems to be of crucial importance.					
30373002	3	66	theme	spray-	594:599	arg1	techniques					618:627	either spray- or freeze-drying techniques	587:627	either spray- or freeze-drying techniques	587:627	The powders, generated by either spray- or freeze-drying techniques, were subsequently subjected to storage testing, and in vitro digestion in simulated stomach and small intestine.					
30373002	6	67	theme	skimmed	994:1000	arg1	cells					1029:1033	skimmed milk-protected spray-dried cells	994:1033	skimmed milk-protected spray-dried cells	994:1033	Moreover, skimmed milk-protected spray-dried cells exhibit the highest resistance to harsh environmental conditions of stomach (53.9 ± 7.6% survival rate) and higher adhesion ability to HT-29 cell line after digestion (528 ± 29 cells per 100 epithelial cells).					
30734626	11	0	with	use	1522:1524	arg1	level					1492:1496	a high bio-safety level	1474:1496	a high bio-safety level	1474:1496	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	6	1	theme	time	827:830	arg1	18.23 hr					832:839	fermentation time 18.23 hr	814:839	fermentation time 18.23 hr	814:839	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	6	1	theme	time	827:830	arg1	L-1					858:860	glucose 12.65 g L-1	842:860	glucose 12.65 g L-1	842:860	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	6	1	theme	time	827:830	arg1	pH					871:872	initial pH 5.18	863:877	initial pH 5.18	863:877	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	9	2	theme	larger	1170:1175	arg1	zone					1187:1190	larger clearance zone	1170:1190	larger clearance zone	1170:1190	This increase could also be qualitatively detected by larger clearance zone on konjac powder-MRS agar through Congo Red dyeing.					
30734626	6	3	theme	12.65 g	850:856	arg1	18.23 hr					832:839	fermentation time 18.23 hr	814:839	fermentation time 18.23 hr	814:839	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	6	3	theme	12.65 g	850:856	arg1	L-1					858:860	glucose 12.65 g L-1	842:860	glucose 12.65 g L-1	842:860	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	11	4	theme	high	1476:1479	arg1	level					1492:1496	a high bio-safety level	1474:1496	a high bio-safety level	1474:1496	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	5	5	dep	L.	688:689	arg1	casei					691:695	casei	691:695	casei	691:695	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	9	6	theme	clearance	1177:1185	arg1	zone					1187:1190	larger clearance zone	1170:1190	larger clearance zone	1170:1190	This increase could also be qualitatively detected by larger clearance zone on konjac powder-MRS agar through Congo Red dyeing.					
30734626	2	7	used	used	278:281	arg2	strains					308:314	β-mannanase-producing strains	286:314	β-mannanase-producing strains	286:314	LAB can heterogeneously express β-mannanase or be directly used as β-mannanase-producing strains.					
30734626	2	7	used	used	278:281	arg2	LAB					219:221	LAB	219:221	LAB	219:221	LAB can heterogeneously express β-mannanase or be directly used as β-mannanase-producing strains.					
30734626	11	8	theme	juice	1529:1533	arg1	clarification					1535:1547	juice clarification	1529:1547	juice clarification	1529:1547	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	10	9	theme	enzyme	1379:1384	arg1	treatment					1386:1394	enzyme treatment	1379:1394	enzyme treatment	1379:1394	The yield and clarity of crude β-mannanase-treated juices from orange, apple, and pear were significantly higher than controls without enzyme treatment.					
30734626	5	10	theme	L.	688:689	arg1	activity					716:723	L. casei HDS-01 β-mannanase activity	688:723	L. casei HDS-01 β-mannanase activity	688:723	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	5	11	theme	Two-factorial	521:533	arg1	design					535:540	Two-factorial design	521:540	Two-factorial design	521:540	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	11	12	theme	food-level	1566:1575	arg1	fields					1577:1582	other food-level fields	1560:1582	other food-level fields	1560:1582	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	0	13	theme	juice	109:113	arg1	clarification					115:127	juice clarification	109:127	juice clarification	109:127	The response surface optimization of β-mannanase produced by Lactobacillus casei HDS-01 and its potential in juice clarification.					
30734626	5	14	dep	time	589:592	arg1	i.e.					570:573	i.e.	570:573	i.e.	570:573	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	10	15	theme	juices	1295:1300	arg1	yield					1248:1252	yield	1248:1252	yield	1248:1252	The yield and clarity of crude β-mannanase-treated juices from orange, apple, and pear were significantly higher than controls without enzyme treatment.					
30734626	10	15	theme	juices	1295:1300	arg1	clarity					1258:1264	clarity	1258:1264	clarity	1258:1264	The yield and clarity of crude β-mannanase-treated juices from orange, apple, and pear were significantly higher than controls without enzyme treatment.					
30734626	11	16	from	use	1522:1524	arg1	clarification					1535:1547	juice clarification	1529:1547	juice clarification	1529:1547	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	10	17	theme	β-mannanase-treated	1275:1293	arg1	juices					1295:1300	crude β-mannanase-treated juices	1269:1300	crude β-mannanase-treated juices	1269:1300	The yield and clarity of crude β-mannanase-treated juices from orange, apple, and pear were significantly higher than controls without enzyme treatment.					
30734626	3	18	theme	fermentation	356:367	arg1	condition					369:377	the fermentation condition	352:377	the fermentation condition	352:377	This research originally optimized the fermentation condition for β-mannanase produced by Lactobacillus casei HDS-01.					
30734626	5	19	theme	initial	634:640	arg1	pH					642:643	initial pH	634:643	initial pH (p = 0.0167)	634:656	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	5	19	theme	initial	634:640	arg1	p = 0.0167					646:655	p = 0.0167	646:655	p = 0.0167	646:655	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	9	20	theme	konjac	1195:1200	arg1	agar					1213:1216	konjac powder-MRS agar	1195:1216	konjac powder-MRS agar through Congo Red dyeing	1195:1241	This increase could also be qualitatively detected by larger clearance zone on konjac powder-MRS agar through Congo Red dyeing.					
30734626	10	21	theme	crude	1269:1273	arg1	juices					1295:1300	crude β-mannanase-treated juices	1269:1300	crude β-mannanase-treated juices	1269:1300	The yield and clarity of crude β-mannanase-treated juices from orange, apple, and pear were significantly higher than controls without enzyme treatment.					
30734626	7	22	theme	subsequent	1012:1021	arg1	p = 0.6308					1031:1040	p = 0.6308	1031:1040	p = 0.6308	1031:1040	This model was validated by conducting six repeated experiments and subsequent t-test (p = 0.6308).					
30734626	7	22	theme	subsequent	1012:1021	arg1	t-test					1023:1028	subsequent t-test	1012:1028	subsequent t-test (p = 0.6308)	1012:1041	This model was validated by conducting six repeated experiments and subsequent t-test (p = 0.6308).					
30734626	0	23	theme	response	4:11	arg1	optimization					21:32	The response surface optimization	0:32	The response surface optimization of β-mannanase	0:47	The response surface optimization of β-mannanase produced by Lactobacillus casei HDS-01 and its potential in juice clarification.					
30734626	6	24	theme	initial	863:869	arg1	18.23 hr					832:839	fermentation time 18.23 hr	814:839	fermentation time 18.23 hr	814:839	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	6	24	theme	initial	863:869	arg1	pH					871:872	initial pH 5.18	863:877	initial pH 5.18	863:877	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	11	25	with	strain	1462:1467	arg1	level					1492:1496	a high bio-safety level	1474:1496	a high bio-safety level	1474:1496	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	9	26	theme	powder-MRS	1202:1211	arg1	agar					1213:1216	konjac powder-MRS agar	1195:1216	konjac powder-MRS agar through Congo Red dyeing	1195:1241	This increase could also be qualitatively detected by larger clearance zone on konjac powder-MRS agar through Congo Red dyeing.					
30734626	6	27	dep	18.23 hr	832:839	arg1	i.e.					808:811	i.e.	808:811	i.e.	808:811	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	11	28	theme	high	1431:1434	arg1	strain					1462:1467	a relatively high β-mannanase-producing LAB strain	1418:1467	a relatively high β-mannanase-producing LAB strain with a high bio-safety level	1418:1496	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	1	29	theme	ideal	163:167	arg1	bacteria					142:149	Lactic acid bacteria	130:149	Lactic acid bacteria (LAB)	130:155	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	1	29	theme	ideal	163:167	arg1	source					179:184	an ideal mannanase source	160:184	an ideal mannanase source due to the bio-safety guarantee	160:216	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	3	30	theme	Lactobacillus	407:419	arg1	casei					421:425	Lactobacillus casei HDS-01	407:432	Lactobacillus casei HDS-01	407:432	This research originally optimized the fermentation condition for β-mannanase produced by Lactobacillus casei HDS-01.					
30734626	8	31	theme	RSM	1044:1046	arg1	optimization					1048:1059	RSM optimization	1044:1059	RSM optimization	1044:1059	RSM optimization obtained a 1.33-fold increase in β-mannanase activity.					
30734626	6	32	theme	design	794:799	arg1	CCD					802:804	the central composite design (CCD)	772:805	the central composite design (CCD)	772:805	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	11	33	theme	β-mannanase-producing	1436:1456	arg1	strain					1462:1467	a relatively high β-mannanase-producing LAB strain	1418:1467	a relatively high β-mannanase-producing LAB strain with a high bio-safety level	1418:1496	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	1	34	theme	mannanase	169:177	arg1	bacteria					142:149	Lactic acid bacteria	130:149	Lactic acid bacteria (LAB)	130:155	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	1	34	theme	mannanase	169:177	arg1	source					179:184	an ideal mannanase source	160:184	an ideal mannanase source due to the bio-safety guarantee	160:216	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	11	35	with	fields	1577:1582	arg1	level					1492:1496	a high bio-safety level	1474:1496	a high bio-safety level	1474:1496	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	5	36	theme	HDS-01	697:702	arg1	activity					716:723	L. casei HDS-01 β-mannanase activity	688:723	L. casei HDS-01 β-mannanase activity	688:723	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	6	37	theme	composite	784:792	arg1	CCD					802:804	the central composite design (CCD)	772:805	the central composite design (CCD)	772:805	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	2	38	theme	β-mannanase-producing	286:306	arg1	LAB					219:221	LAB	219:221	LAB	219:221	LAB can heterogeneously express β-mannanase or be directly used as β-mannanase-producing strains.					
30734626	2	38	theme	β-mannanase-producing	286:306	arg1	strains					308:314	β-mannanase-producing strains	286:314	β-mannanase-producing strains	286:314	LAB can heterogeneously express β-mannanase or be directly used as β-mannanase-producing strains.					
30734626	10	39	dep	yield	1248:1252	arg1	The					1244:1246	The	1244:1246	The	1244:1246	The yield and clarity of crude β-mannanase-treated juices from orange, apple, and pear were significantly higher than controls without enzyme treatment.					
30734626	9	40	theme	Red	1232:1234	arg1	dyeing					1236:1241	Congo Red dyeing	1226:1241	Congo Red dyeing	1226:1241	This increase could also be qualitatively detected by larger clearance zone on konjac powder-MRS agar through Congo Red dyeing.					
30734626	0	41	theme	surface	13:19	arg1	optimization					21:32	The response surface optimization	0:32	The response surface optimization of β-mannanase	0:47	The response surface optimization of β-mannanase produced by Lactobacillus casei HDS-01 and its potential in juice clarification.					
30734626	5	42	theme	β-mannanase	704:714	arg1	activity					716:723	L. casei HDS-01 β-mannanase activity	688:723	L. casei HDS-01 β-mannanase activity	688:723	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	4	43	theme	enzyme	473:478	arg1	potential					450:458	The applicable potential	435:458	The applicable potential of the crude enzyme in juice clarification	435:501	The applicable potential of the crude enzyme in juice clarification was investigated.					
30734626	11	44	from	fields	1577:1582	arg1	clarification					1535:1547	juice clarification	1529:1547	juice clarification	1529:1547	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	1	45	theme	due	186:188	arg1	bacteria					142:149	Lactic acid bacteria	130:149	Lactic acid bacteria (LAB)	130:155	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	1	45	theme	due	186:188	arg1	source					179:184	an ideal mannanase source	160:184	an ideal mannanase source due to the bio-safety guarantee	160:216	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	0	46	theme	β-mannanase	37:47	arg1	optimization					21:32	The response surface optimization	0:32	The response surface optimization of β-mannanase	0:47	The response surface optimization of β-mannanase produced by Lactobacillus casei HDS-01 and its potential in juice clarification.					
30734626	6	47	theme	mL-1	938:941	arg1	activity					918:925	maximal β-mannanase activity	898:925	maximal β-mannanase activity of 81.40 U mL-1	898:941	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	4	48	theme	crude	467:471	arg1	enzyme					473:478	the crude enzyme	463:478	the crude enzyme	463:478	The applicable potential of the crude enzyme in juice clarification was investigated.					
30734626	11	49	from	strain	1462:1467	arg1	clarification					1535:1547	juice clarification	1529:1547	juice clarification	1529:1547	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	11	50	theme	LAB	1458:1460	arg1	strain					1462:1467	a relatively high β-mannanase-producing LAB strain	1418:1467	a relatively high β-mannanase-producing LAB strain with a high bio-safety level	1418:1496	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	6	51	theme	81.40 U	930:936	arg1	mL-1					938:941	81.40 U mL-1	930:941	81.40 U mL-1	930:941	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	8	52	theme	1.33-fold	1072:1080	arg1	increase					1082:1089	a 1.33-fold increase	1070:1089	a 1.33-fold increase in β-mannanase activity	1070:1113	RSM optimization obtained a 1.33-fold increase in β-mannanase activity.					
30734626	4	53	theme	juice	483:487	arg1	clarification					489:501	juice clarification	483:501	juice clarification	483:501	The applicable potential of the crude enzyme in juice clarification was investigated.					
30734626	6	54	theme	central	776:782	arg1	CCD					802:804	the central composite design (CCD)	772:805	the central composite design (CCD)	772:805	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	9	55	located	detected	1158:1165	arg1	agar					1213:1216	konjac powder-MRS agar	1195:1216	konjac powder-MRS agar through Congo Red dyeing	1195:1241	This increase could also be qualitatively detected by larger clearance zone on konjac powder-MRS agar through Congo Red dyeing.					
30734626	9	55	located	detected	1158:1165	arg2	increase					1121:1128	This increase	1116:1128	This increase	1116:1128	This increase could also be qualitatively detected by larger clearance zone on konjac powder-MRS agar through Congo Red dyeing.					
30734626	8	56	theme	β-mannanase	1094:1104	arg1	activity					1106:1113	β-mannanase activity	1094:1113	β-mannanase activity	1094:1113	RSM optimization obtained a 1.33-fold increase in β-mannanase activity.					
30734626	6	57	theme	glucose	842:848	arg1	18.23 hr					832:839	fermentation time 18.23 hr	814:839	fermentation time 18.23 hr	814:839	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	6	57	theme	glucose	842:848	arg1	L-1					858:860	glucose 12.65 g L-1	842:860	glucose 12.65 g L-1	842:860	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	5	58	theme	fermentation	576:587	arg1	p = 0.0001					595:604	p = 0.0001	595:604	p = 0.0001	595:604	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	5	58	theme	fermentation	576:587	arg1	time					589:592	fermentation time	576:592	fermentation time (p = 0.0001)	576:605	Two-factorial design screened out three factors, i.e., fermentation time (p = 0.0001), glucose (p = 0.0013), and initial pH (p = 0.0167), which significantly affected L. casei HDS-01 β-mannanase activity.					
30734626	0	59	from	casei	75:79	arg1	clarification					115:127	juice clarification	109:127	juice clarification	109:127	The response surface optimization of β-mannanase produced by Lactobacillus casei HDS-01 and its potential in juice clarification.					
30734626	6	60	theme	β-mannanase	906:916	arg1	activity					918:925	maximal β-mannanase activity	898:925	maximal β-mannanase activity of 81.40 U mL-1	898:941	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	4	61	from	potential	450:458	arg1	clarification					489:501	juice clarification	483:501	juice clarification	483:501	The applicable potential of the crude enzyme in juice clarification was investigated.					
30734626	4	62	theme	applicable	439:448	arg1	potential					450:458	The applicable potential	435:458	The applicable potential of the crude enzyme in juice clarification	435:501	The applicable potential of the crude enzyme in juice clarification was investigated.					
30734626	10	63	from	orange	1307:1312	arg1	yield					1248:1252	yield	1248:1252	yield	1248:1252	The yield and clarity of crude β-mannanase-treated juices from orange, apple, and pear were significantly higher than controls without enzyme treatment.					
30734626	10	63	from	orange	1307:1312	arg1	clarity					1258:1264	clarity	1258:1264	clarity	1258:1264	The yield and clarity of crude β-mannanase-treated juices from orange, apple, and pear were significantly higher than controls without enzyme treatment.					
30734626	7	64	theme	repeated	987:994	arg1	experiments					996:1006	six repeated experiments	983:1006	six repeated experiments	983:1006	This model was validated by conducting six repeated experiments and subsequent t-test (p = 0.6308).					
30734626	1	65	theme	Lactic	130:135	arg1	LAB					152:154	LAB	152:154	LAB	152:154	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	1	65	theme	Lactic	130:135	arg1	source					179:184	an ideal mannanase source	160:184	an ideal mannanase source due to the bio-safety guarantee	160:216	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	1	65	theme	Lactic	130:135	arg1	bacteria					142:149	Lactic acid bacteria	130:149	Lactic acid bacteria (LAB)	130:155	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	11	66	theme	other	1560:1564	arg1	fields					1577:1582	other food-level fields	1560:1582	other food-level fields	1560:1582	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	1	67	theme	bio-safety	197:206	arg1	guarantee					208:216	the bio-safety guarantee	193:216	the bio-safety guarantee	193:216	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	0	68	from	potential	96:104	arg1	clarification					115:127	juice clarification	109:127	juice clarification	109:127	The response surface optimization of β-mannanase produced by Lactobacillus casei HDS-01 and its potential in juice clarification.					
30734626	1	69	theme	acid	137:140	arg1	LAB					152:154	LAB	152:154	LAB	152:154	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	1	69	theme	acid	137:140	arg1	source					179:184	an ideal mannanase source	160:184	an ideal mannanase source due to the bio-safety guarantee	160:216	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	1	69	theme	acid	137:140	arg1	bacteria					142:149	Lactic acid bacteria	130:149	Lactic acid bacteria (LAB)	130:155	Lactic acid bacteria (LAB) is an ideal mannanase source due to the bio-safety guarantee.					
30734626	11	70	theme	bio-safety	1481:1490	arg1	level					1492:1496	a high bio-safety level	1474:1496	a high bio-safety level	1474:1496	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	6	71	theme	fermentation	814:825	arg1	18.23 hr					832:839	fermentation time 18.23 hr	814:839	fermentation time 18.23 hr	814:839	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	6	71	theme	fermentation	814:825	arg1	L-1					858:860	glucose 12.65 g L-1	842:860	glucose 12.65 g L-1	842:860	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	6	71	theme	fermentation	814:825	arg1	pH					871:872	initial pH 5.18	863:877	initial pH 5.18	863:877	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	11	72	theme	economical	1511:1520	arg1	use					1522:1524	easy and economical use	1502:1524	easy and economical use in juice clarification	1502:1547	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	6	73	theme	predicted	736:744	arg1	conditions					746:755	the predicted conditions	732:755	the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18	732:877	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	11	74	theme	easy	1502:1505	arg1	use					1522:1524	easy and economical use	1502:1524	easy and economical use in juice clarification	1502:1547	This study conferred a relatively high β-mannanase-producing LAB strain with a high bio-safety level and easy and economical use in juice clarification as well as other food-level fields.					
30734626	0	75	theme	Lactobacillus	61:73	arg1	casei					75:79	Lactobacillus casei HDS-01	61:86	Lactobacillus casei HDS-01	61:86	The response surface optimization of β-mannanase produced by Lactobacillus casei HDS-01 and its potential in juice clarification.					
30734626	6	76	theme	maximal	898:904	arg1	activity					918:925	maximal β-mannanase activity	898:925	maximal β-mannanase activity of 81.40 U mL-1	898:941	Under the predicted conditions resulting from the central composite design (CCD), i.e., fermentation time 18.23 hr, glucose 12.65 g L-1, initial pH 5.18, the model reached maximal β-mannanase activity of 81.40 U mL-1.					
30734626	8	77	from	increase	1082:1089	arg1	activity					1106:1113	β-mannanase activity	1094:1113	β-mannanase activity	1094:1113	RSM optimization obtained a 1.33-fold increase in β-mannanase activity.					
29628249	0	0	theme	tissue	94:99	arg1	engineering					101:111	bone tissue engineering	89:111	bone tissue engineering	89:111	Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering.					
29628249	7	1	theme	bioactivity	1184:1194	arg1	properties					1196:1205	favorable physicochemical and bioactivity properties	1154:1205	favorable physicochemical and bioactivity properties	1154:1205	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	6	2	theme	alkaline	1049:1056	arg1	ALP					1071:1073	ALP	1071:1073	ALP	1071:1073	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	6	2	theme	alkaline	1049:1056	arg1	phosphatase					1058:1068	alkaline phosphatase	1049:1068	alkaline phosphatase (ALP)	1049:1074	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	3	3	theme	nBGC	509:512	arg1	nanoparticles					514:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles	460:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles	460:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	6	4	theme	human	1006:1010	arg1	HOBs					1025:1028	HOBs	1025:1028	HOBs	1025:1028	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	6	4	theme	human	1006:1010	arg1	osteoblasts					1012:1022	human osteoblasts	1006:1022	human osteoblasts (HOBs) proliferation	1006:1043	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	0	5	theme	bone	89:92	arg1	engineering					101:111	bone tissue engineering	89:111	bone tissue engineering	89:111	Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering.					
29628249	2	6	theme	electron	331:338	arg1	microscopy					340:349	Scanning electron microscopy	322:349	Scanning electron microscopy	322:349	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	1	7	theme	wt	248:249	arg1	0-16					242:245	0-16	242:245	0-16	242:245	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	1	7	theme	wt	248:249	arg1	%					250:250	wt%	248:250	wt%	248:250	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	7	8	theme	promising	1259:1267	arg1	hydrogels					1231:1239	the Dex/nBGC composite hydrogels	1208:1239	the Dex/nBGC composite hydrogels	1208:1239	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	7	8	theme	promising	1259:1267	arg1	scaffolds					1279:1287	promising bioactive scaffolds	1259:1287	promising bioactive scaffolds for bone tissue engineering applications	1259:1328	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	7	9	theme	bone	1293:1296	arg1	applications					1317:1328	bone tissue engineering applications	1293:1328	bone tissue engineering applications	1293:1328	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	4	10	theme	higher	732:737	arg1	contents					744:751	higher nBGC contents	732:751	higher nBGC contents	732:751	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	5	11	theme	body	937:940	arg1	fluid					942:946	the simulated body fluid	923:946	the simulated body fluid	923:946	Bioactivity of Dex/nBGC scaffolds was validated through apatite formation after submersion in the simulated body fluid.					
29628249	7	12	theme	composite	1221:1229	arg1	hydrogels					1231:1239	the Dex/nBGC composite hydrogels	1208:1239	the Dex/nBGC composite hydrogels	1208:1239	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	7	12	theme	composite	1221:1229	arg1	scaffolds					1279:1287	promising bioactive scaffolds	1259:1287	promising bioactive scaffolds for bone tissue engineering applications	1259:1328	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	3	13	theme	Dex	569:571	arg1	matrix					573:578	the Dex matrix	565:578	the Dex matrix	565:578	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	5	14	theme	simulated	927:935	arg1	fluid					942:946	the simulated body fluid	923:946	the simulated body fluid	923:946	Bioactivity of Dex/nBGC scaffolds was validated through apatite formation after submersion in the simulated body fluid.					
29628249	3	15	theme	spectroscopy	484:495	arg1	nanoparticles					514:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles	460:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles	460:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	1	16	theme	glass	207:211	arg1	nanoparticles					221:233	derived bioactive glass ceramic nanoparticles	189:233	derived bioactive glass ceramic nanoparticles	189:233	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	4	17	theme	increased	762:770	arg1	uptake					778:783	increased water uptake	762:783	increased water uptake	762:783	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	5	18	theme	Dex/nBGC	844:851	arg1	scaffolds					853:861	Dex/nBGC scaffolds	844:861	Dex/nBGC scaffolds	844:861	Bioactivity of Dex/nBGC scaffolds was validated through apatite formation after submersion in the simulated body fluid.					
29628249	7	19	theme	favorable	1154:1162	arg1	properties					1196:1205	favorable physicochemical and bioactivity properties	1154:1205	favorable physicochemical and bioactivity properties	1154:1205	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	2	20	with	microstructure	408:421	arg1	size					444:447	an average pore size	428:447	an average pore size of 240 μm	428:457	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	1	21	link	derived	189:195	arg1	nanoparticles					221:233	derived bioactive glass ceramic nanoparticles	189:233	derived bioactive glass ceramic nanoparticles	189:233	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	7	22	theme	physicochemical	1164:1178	arg1	properties					1196:1205	favorable physicochemical and bioactivity properties	1154:1205	favorable physicochemical and bioactivity properties	1154:1205	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	2	23	dep	showed	351:356	arg1	consisting					382:391	consisting	382:391	showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm	351:457	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	5	24	theme	scaffolds	853:861	arg1	Bioactivity					829:839	Bioactivity	829:839	Bioactivity of Dex/nBGC scaffolds	829:861	Bioactivity of Dex/nBGC scaffolds was validated through apatite formation after submersion in the simulated body fluid.					
29628249	2	25	theme	pore	439:442	arg1	size					444:447	an average pore size	428:447	an average pore size of 240 μm	428:457	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	6	26	theme	composite	958:966	arg1	scaffolds					968:976	Dex/nBGC composite scaffolds	949:976	Dex/nBGC composite scaffolds	949:976	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	7	27	theme	bioactive	1269:1277	arg1	hydrogels					1231:1239	the Dex/nBGC composite hydrogels	1208:1239	the Dex/nBGC composite hydrogels	1208:1239	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	7	27	theme	bioactive	1269:1277	arg1	scaffolds					1279:1287	promising bioactive scaffolds	1259:1287	promising bioactive scaffolds for bone tissue engineering applications	1259:1328	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	3	28	theme	low	583:585	arg1	content					592:598	low nBGC content	583:598	low nBGC content (2 wt%)	583:606	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	3	28	theme	low	583:585	arg1	%					605:605	2 wt%	601:605	2 wt%	601:605	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	6	29	dep	16	1120:1121	arg1	to					1117:1118	to	1117:1118	to	1117:1118	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	7	30	theme	tissue	1298:1303	arg1	applications					1317:1328	bone tissue engineering applications	1293:1328	bone tissue engineering applications	1293:1328	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	2	31	theme	average	431:437	arg1	size					444:447	an average pore size	428:447	an average pore size of 240 μm	428:457	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	7	32	theme	Dex/nBGC	1212:1219	arg1	hydrogels					1231:1239	the Dex/nBGC composite hydrogels	1208:1239	the Dex/nBGC composite hydrogels	1208:1239	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	7	32	theme	Dex/nBGC	1212:1219	arg1	scaffolds					1279:1287	promising bioactive scaffolds	1259:1287	promising bioactive scaffolds for bone tissue engineering applications	1259:1328	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	5	33	from	submersion	909:918	arg1	fluid					942:946	the simulated body fluid	923:946	the simulated body fluid	923:946	Bioactivity of Dex/nBGC scaffolds was validated through apatite formation after submersion in the simulated body fluid.					
29628249	3	34	theme	nBGC	587:590	arg1	content					592:598	low nBGC content	583:598	low nBGC content (2 wt%)	583:606	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	3	34	theme	nBGC	587:590	arg1	%					605:605	2 wt%	601:605	2 wt%	601:605	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	1	35	theme	derived	189:195	arg1	nanoparticles					221:233	derived bioactive glass ceramic nanoparticles	189:233	derived bioactive glass ceramic nanoparticles	189:233	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	4	36	from	contents	744:751	arg1	pressure					697:704	osmotic pressure	689:704	osmotic pressure	689:704	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	4	36	from	contents	744:751	arg1	agglomeration					715:727	nBGC agglomeration	710:727	nBGC agglomeration	710:727	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	4	37	theme	nBGC	739:742	arg1	contents					744:751	higher nBGC contents	732:751	higher nBGC contents	732:751	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	1	38	theme	bioactive	197:205	arg1	nanoparticles					221:233	derived bioactive glass ceramic nanoparticles	189:233	derived bioactive glass ceramic nanoparticles	189:233	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	1	39	dep	dextran	163:169	arg1	nBGC					236:239	nBGC	236:239	nBGC: 0-16 (wt%)	236:251	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	1	39	dep	dextran	163:169	arg1	nanoparticles					221:233	derived bioactive glass ceramic nanoparticles	189:233	derived bioactive glass ceramic nanoparticles	189:233	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	2	40	theme	3D	405:406	arg1	microstructure					408:421	a porous 3D microstructure	396:421	a porous 3D microstructure with an average pore size of 240 μm	396:457	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	0	41	theme	glass-ceramic	46:58	arg1	scaffolds					75:83	bioactive glass-ceramic: Nanocomposite scaffolds	36:83	bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering	36:111	Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering.					
29628249	3	42	theme	higher	645:650	arg1	contents					657:664	higher nBGC contents	645:664	higher nBGC contents	645:664	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	2	43	theme	porous	398:403	arg1	microstructure					408:421	a porous 3D microstructure	396:421	a porous 3D microstructure with an average pore size of 240 μm	396:457	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	7	44	theme	engineering	1305:1315	arg1	applications					1317:1328	bone tissue engineering applications	1293:1328	bone tissue engineering applications	1293:1328	Owing to favorable physicochemical and bioactivity properties, the Dex/nBGC composite hydrogels can be offered as promising bioactive scaffolds for bone tissue engineering applications.					
29628249	1	45	theme	ceramic	213:219	arg1	nanoparticles					221:233	derived bioactive glass ceramic nanoparticles	189:233	derived bioactive glass ceramic nanoparticles	189:233	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	0	46	theme	bioactive	36:44	arg1	scaffolds					75:83	bioactive glass-ceramic: Nanocomposite scaffolds	36:83	bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering	36:111	Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering.					
29628249	1	47	theme	bioactive	273:281	arg1	scaffolds					283:291	bioactive scaffolds	273:291	bioactive scaffolds for bone tissue engineering	273:319	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	1	47	theme	bioactive	273:281	arg1	series					116:121	A series	114:121	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%))	114:252	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	2	48	theme	240 μm	452:457	arg1	size					444:447	an average pore size	428:447	an average pore size of 240 μm	428:457	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	6	49	theme	improved	997:1004	arg1	activity					1076:1083	improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity	997:1083	improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity	997:1083	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	6	50	theme	proliferation	1031:1043	arg1	activity					1076:1083	improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity	997:1083	improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity	997:1083	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	0	51	theme	Nanocomposite	61:73	arg1	scaffolds					75:83	bioactive glass-ceramic: Nanocomposite scaffolds	36:83	bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering	36:111	Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering.					
29628249	6	52	theme	Dex/nBGC	949:956	arg1	scaffolds					968:976	Dex/nBGC composite scaffolds	949:976	Dex/nBGC composite scaffolds	949:976	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	1	53	dep	nBGC	236:239	arg1	0-16					242:245	0-16	242:245	0-16	242:245	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	1	53	dep	nBGC	236:239	arg1	%					250:250	wt%	248:250	wt%	248:250	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	4	54	theme	compressive	808:818	arg1	modulus					820:826	the compressive modulus	804:826	the compressive modulus	804:826	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	2	55	theme	Scanning	322:329	arg1	microscopy					340:349	Scanning electron microscopy	322:349	Scanning electron microscopy	322:349	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	4	56	theme	modulus	820:826	arg1	reduction					791:799	reduction	791:799	reduction of the compressive modulus	791:826	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	1	57	theme	nanocomposite	126:138	arg1	scaffolds					140:148	nanocomposite scaffolds	126:148	nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%))	126:252	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	4	58	theme	nBGC	710:713	arg1	agglomeration					715:727	nBGC agglomeration	710:727	nBGC agglomeration	710:727	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	1	59	theme	bone	297:300	arg1	engineering					309:319	bone tissue engineering	297:319	bone tissue engineering	297:319	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	1	60	theme	scaffolds	140:148	arg1	scaffolds					283:291	bioactive scaffolds	273:291	bioactive scaffolds for bone tissue engineering	273:319	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	1	60	theme	scaffolds	140:148	arg1	series					116:121	A series	114:121	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%))	114:252	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	4	61	dep	pressure	697:704	arg1	the					685:687	the	685:687	the	685:687	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	6	62	theme	wt	1124:1125	arg1	content					1106:1112	nBGC content up to 16	1101:1121	nBGC content up to 16 (wt%)	1101:1127	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	6	62	theme	wt	1124:1125	arg1	%					1126:1126	wt%	1124:1126	wt%	1124:1126	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	3	63	theme	nBGC	652:655	arg1	contents					657:664	higher nBGC contents	645:664	higher nBGC contents	645:664	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	4	64	theme	water	772:776	arg1	uptake					778:783	increased water uptake	762:783	increased water uptake	762:783	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
29628249	3	65	theme	x-ray	478:482	arg1	spectroscopy					484:495	Energy-dispersive x-ray spectroscopy	460:495	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles	460:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	3	66	theme	Energy-dispersive	460:476	arg1	spectroscopy					484:495	Energy-dispersive x-ray spectroscopy	460:495	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles	460:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	6	67	theme	nBGC	1101:1104	arg1	content					1106:1112	nBGC content up to 16	1101:1121	nBGC content up to 16 (wt%)	1101:1127	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	6	67	theme	nBGC	1101:1104	arg1	%					1126:1126	wt%	1124:1126	wt%	1124:1126	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	0	68	dep	hydrogels	8:16	arg1	incorporated					18:29	incorporated	18:29	hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering	8:111	Dextran hydrogels incorporated with bioactive glass-ceramic: Nanocomposite scaffolds for bone tissue engineering.					
29628249	6	69	theme	phosphatase	1058:1068	arg1	activity					1076:1083	improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity	997:1083	improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity	997:1083	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	2	70	theme	Dex/nBGC	358:365	arg1	scaffolds					367:375	Dex/nBGC scaffolds	358:375	Dex/nBGC scaffolds	358:375	Scanning electron microscopy showed Dex/nBGC scaffolds were consisting of a porous 3D microstructure with an average pore size of 240 μm.					
29628249	3	71	located	observed	633:640	arg2	agglomeration					615:627	agglomeration	615:627	agglomeration	615:627	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	3	71	located	observed	633:640	arg1	contents					657:664	higher nBGC contents	645:664	higher nBGC contents	645:664	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	1	72	theme	tissue	302:307	arg1	engineering					309:319	bone tissue engineering	297:319	bone tissue engineering	297:319	A series of nanocomposite scaffolds comprised of dextran (Dex) and sol-gel derived bioactive glass ceramic nanoparticles (nBGC: 0-16 (wt%)) were fabricated as bioactive scaffolds for bone tissue engineering.					
29628249	6	73	theme	osteoblasts	1012:1022	arg1	proliferation					1031:1043	human osteoblasts (HOBs) proliferation	1006:1043	human osteoblasts (HOBs) proliferation	1006:1043	Dex/nBGC composite scaffolds were found to show improved human osteoblasts (HOBs) proliferation and alkaline phosphatase (ALP) activity with increasing nBGC content up to 16 (wt%) over two weeks.					
29628249	5	74	theme	apatite	885:891	arg1	formation					893:901	apatite formation	885:901	apatite formation	885:901	Bioactivity of Dex/nBGC scaffolds was validated through apatite formation after submersion in the simulated body fluid.					
29628249	3	75	theme	illustrated	497:507	arg1	nanoparticles					514:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles	460:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles	460:526	Energy-dispersive x-ray spectroscopy illustrated nBGC nanoparticles were homogenously distributed within the Dex matrix at low nBGC content (2 wt%), while agglomeration was observed at higher nBGC contents.					
29628249	4	76	theme	osmotic	689:695	arg1	pressure					697:704	osmotic pressure	689:704	osmotic pressure	689:704	It was found that the osmotic pressure and nBGC agglomeration at higher nBGC contents leads to increased water uptake, then reduction of the compressive modulus.					
31520999	8	0	theme	chaperone	1887:1895	arg1	protocol					1897:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	3	1	from	BSA	892:894	arg1	presence					903:910	the presence	899:910	the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	899:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	8	2	theme	efficient	2065:2073	arg1	aides					2099:2103	efficient and inexpensive folding aides	2065:2103	efficient and inexpensive folding aides	2065:2103	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	6	3	theme	methyl-β-cyclodextrin	1534:1554	arg1	existence					1521:1529	the existence	1517:1529	the existence of methyl-β-cyclodextrin	1517:1554	But overall, CTAB has the better renaturation tendency as compare to SDS in the existence of methyl-β-cyclodextrin.					
31520999	1	4	theme	present	190:196	arg1	study					198:202	the present study	186:202	the present study	186:202	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	7	5	contain	carries	1766:1772	arg1	BSA					1762:1764	BSA	1762:1764	BSA	1762:1764	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	7	5	contain	carries	1766:1772	arg3	pH 7.4					1746:1751	pH 7.4	1746:1751	pH 7.4	1746:1751	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	7	5	contain	carries	1766:1772	arg2	charge					1783:1788	negative charge	1774:1788	negative charge on their surface	1774:1805	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	1	6	theme	mixture	487:493	arg1	presence					346:353	the presence	342:353	the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	342:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	7	7	theme	surfactant	1653:1662	arg1	tail					1640:1643	hydrophobic tail	1628:1643	hydrophobic tail of CTAB surfactant	1628:1662	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	0	8	theme	artificial	131:140	arg1	protocol					152:159	artificial chaperone protocol	131:159	artificial chaperone protocol	131:159	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	1	9	theme	artificial	575:584	arg1	method					614:619	artificial chaperone-assisted two-step method	575:619	artificial chaperone-assisted two-step method	575:619	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	2	10	theme	surfactants	790:800	arg1	presence					760:767	the presence	756:767	the presence of cationic, anionic surfactants	756:800	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	2	11	from	presence	760:767	arg1	times					735:739	200 times	731:739	200 times of dilution in the presence of cationic, anionic surfactants	731:800	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	2	11	from	presence	760:767	arg1	mixture					823:829	their catanionic mixture	806:829	their catanionic mixture	806:829	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	8	12	theme	folding	2091:2097	arg1	aides					2099:2103	efficient and inexpensive folding aides	2065:2103	efficient and inexpensive folding aides	2065:2103	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	1	13	theme	cooperative	219:229	arg1	behaviour					254:262	the cooperative refolding/renaturation behaviour	215:262	the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	215:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	7	14	theme	hydrophobic	1628:1638	arg1	tail					1640:1643	hydrophobic tail	1628:1643	hydrophobic tail of CTAB surfactant	1628:1662	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	0	15	theme	Biophysical	162:172	arg1	insight					174:180	Biophysical insight	162:180	Biophysical insight	162:180	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	1	16	theme	surfactant	425:434	arg1	SDS					461:463	SDS	461:463	SDS	461:463	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	16	theme	surfactant	425:434	arg1	sulphate					451:458	anionic surfactant sodium dodecyl sulphate	417:458	anionic surfactant sodium dodecyl sulphate (SDS)	417:464	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	7	17	theme	length	1618:1623	arg1	presence					1584:1591	the presence	1580:1591	the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface	1580:1805	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	1	18	from	behaviour	254:262	arg1	presence					346:353	the presence	342:353	the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	342:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	19	theme	dodecyl	443:449	arg1	SDS					461:463	SDS	461:463	SDS	461:463	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	19	theme	dodecyl	443:449	arg1	sulphate					451:458	anionic surfactant sodium dodecyl sulphate	417:458	anionic surfactant sodium dodecyl sulphate (SDS)	417:464	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	5	20	theme	renaturation	1298:1309	arg1	tendency					1311:1318	a good renaturation tendency	1291:1318	a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions	1291:1438	On other hand, CTAB and SDS are more effective and reflect a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions.					
31520999	3	21	theme	minimum	863:869	arg1	refolding					871:879	minimum refolding	863:879	minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	863:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	7	22	theme	group	1608:1612	arg1	presence					1584:1591	the presence	1580:1591	the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface	1580:1805	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	4	23	theme	effective	1150:1158	arg1	role					1160:1163	an effective role	1147:1163	an effective role	1147:1163	Other mixtures (CTAB/SDS = 20/80 and 80/20) slightly played an effective role during refolding process in the presence of methyl-β-cyclodextrin.					
31520999	5	24	theme	BSA	1333:1335	arg1	solution					1337:1344	denatured BSA solution	1323:1344	denatured BSA solution	1323:1344	On other hand, CTAB and SDS are more effective and reflect a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions.					
31520999	8	25	theme	biophysical	1814:1824	arg1	parameters					1826:1835	These biophysical parameters	1808:1835	These biophysical parameters	1808:1835	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	7	26	theme	charge	1596:1601	arg1	group					1608:1612	charge head group	1596:1612	charge head group	1596:1612	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	2	27	theme	dilution	744:751	arg1	times					735:739	200 times	731:739	200 times of dilution in the presence of cationic, anionic surfactants	731:800	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	2	27	theme	dilution	744:751	arg1	mixture					823:829	their catanionic mixture	806:829	their catanionic mixture	806:829	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	8	28	theme	biotechnological	2014:2029	arg1	industries					2031:2040	biotechnological industries	2014:2040	biotechnological industries	2014:2040	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	3	29	theme	aggregate	1066:1074	arg1	formation					1076:1084	the unordered microstructure aggregate formation	1037:1084	the unordered microstructure aggregate formation	1037:1084	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	3	30	theme	BSA	892:894	arg1	refolding					871:879	minimum refolding	863:879	minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	863:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	1	31	theme	surfactant	367:376	arg1	CTAB					410:413	CTAB	410:413	CTAB	410:413	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	31	theme	surfactant	367:376	arg1	bromide					401:407	cationic surfactant cetyltrimethylammonium bromide	358:407	cationic surfactant cetyltrimethylammonium bromide (CTAB)	358:414	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	3	32	theme	unordered	1041:1049	arg1	microstructure					1051:1064	the unordered microstructure	1037:1064	the unordered microstructure aggregate formation	1037:1084	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	3	33	theme	rational	919:926	arg1	mixture					928:934	1:1 rational mixture	915:934	1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	915:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	1	34	theme	pH 7.4	561:566	arg1	solution					502:509	the solution	498:509	the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	498:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	35	theme	bromide	401:407	arg1	presence					346:353	the presence	342:353	the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	342:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	4	36	dep	mixtures	1093:1100	arg1	80/20					1124:1128	80/20	1124:1128	80/20	1124:1128	Other mixtures (CTAB/SDS = 20/80 and 80/20) slightly played an effective role during refolding process in the presence of methyl-β-cyclodextrin.					
31520999	4	36	dep	mixtures	1093:1100	arg1	CTAB/SDS = 20/80					1103:1118	CTAB/SDS = 20/80	1103:1118	CTAB/SDS = 20/80	1103:1118	Other mixtures (CTAB/SDS = 20/80 and 80/20) slightly played an effective role during refolding process in the presence of methyl-β-cyclodextrin.					
31520999	7	37	theme	important	1678:1686	arg1	task					1688:1691	an important task	1675:1691	an important task	1675:1691	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	2	38	theme	denatured	711:719	arg1	BSA					721:723	denatured BSA	711:723	denatured BSA	711:723	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	0	39	theme	Comparative	0:10	arg1	refolding					12:20	Comparative refolding	0:20	Comparative refolding of guanidinium hydrochloride	0:49	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	1	40	theme	denatured	302:310	arg1	BSA					334:336	BSA	334:336	BSA	334:336	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	40	theme	denatured	302:310	arg1	albumin					325:331	bovine serum albumin	312:331	guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA)	267:337	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	8	41	theme	artificial	1876:1885	arg1	protocol					1897:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	7	42	theme	electrostatic	1700:1712	arg1	interactions					1730:1741	electrostatic and hydrophobic interactions	1700:1741	electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface	1700:1805	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	1	43	theme	chaperone-assisted	586:603	arg1	method					614:619	artificial chaperone-assisted two-step method	575:619	artificial chaperone-assisted two-step method	575:619	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	8	44	theme	inexpensive	2079:2089	arg1	aides					2099:2103	efficient and inexpensive folding aides	2065:2103	efficient and inexpensive folding aides	2065:2103	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	1	45	theme	serum	319:323	arg1	BSA					334:336	BSA	334:336	BSA	334:336	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	45	theme	serum	319:323	arg1	albumin					325:331	bovine serum albumin	312:331	guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA)	267:337	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	0	46	theme	hydrochloride	37:49	arg1	refolding					12:20	Comparative refolding	0:20	Comparative refolding of guanidinium hydrochloride	0:49	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	8	47	theme	aides	2099:2103	arg1	development					2050:2060	the development	2046:2060	the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation	2046:2232	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	8	48	theme	engineered	2153:2162	arg1	cells					2164:2168	genetically engineered cells	2141:2168	genetically engineered cells related diseases, resulting from protein misfolding/aggregation	2141:2232	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	1	49	attach	presence	346:353	arg2	sulphate					451:458	anionic surfactant sodium dodecyl sulphate	417:458	anionic surfactant sodium dodecyl sulphate (SDS)	417:464	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	49	attach	presence	346:353	arg2	mixture					487:493	their catanionic mixture	470:493	their catanionic mixture	470:493	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	49	attach	presence	346:353	arg1	solution					502:509	the solution	498:509	the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	498:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	49	attach	presence	346:353	arg2	CTAB					410:413	CTAB	410:413	CTAB	410:413	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	49	attach	presence	346:353	arg2	SDS					461:463	SDS	461:463	SDS	461:463	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	49	attach	presence	346:353	arg2	bromide					401:407	cationic surfactant cetyltrimethylammonium bromide	358:407	cationic surfactant cetyltrimethylammonium bromide (CTAB)	358:414	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	50	from	presence	346:353	arg1	solution					502:509	the solution	498:509	the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	498:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	4	51	from	process	1182:1188	arg1	presence					1197:1204	the presence	1193:1204	the presence of methyl-β-cyclodextrin	1193:1229	Other mixtures (CTAB/SDS = 20/80 and 80/20) slightly played an effective role during refolding process in the presence of methyl-β-cyclodextrin.					
31520999	7	52	from	charge	1783:1788	arg1	surface					1799:1805	their surface	1793:1805	their surface	1793:1805	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	1	53	theme	60 mM	514:518	arg1	buffer					537:542	60 mM sodium phosphate buffer	514:542	60 mM sodium phosphate buffer	514:542	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	54	theme	refolding/renaturation	231:252	arg1	behaviour					254:262	the cooperative refolding/renaturation behaviour	215:262	the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	215:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	7	55	from	pH 7.4	1746:1751	arg1	interactions					1730:1741	electrostatic and hydrophobic interactions	1700:1741	electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface	1700:1805	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	2	56	dep	cationic	772:779	arg1	anionic					782:788	anionic	782:788	anionic	782:788	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	1	57	theme	phosphate	527:535	arg1	buffer					537:542	60 mM sodium phosphate buffer	514:542	60 mM sodium phosphate buffer	514:542	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	8	58	theme	industries	2031:2040	arg1	problems					2002:2009	the associated problems	1987:2009	the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation	1987:2232	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	6	59	theme	better	1467:1472	arg1	tendency					1487:1494	the better renaturation tendency	1463:1494	the better renaturation tendency	1463:1494	But overall, CTAB has the better renaturation tendency as compare to SDS in the existence of methyl-β-cyclodextrin.					
31520999	3	60	theme	micelles	993:1000	arg1	formation					1002:1010	the micelles formation	989:1010	the micelles formation which is responsible for the unordered microstructure aggregate formation	989:1084	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	3	60	theme	micelles	993:1000	arg1	responsible					1021:1031	responsible	1021:1031	responsible	1021:1031	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	1	61	theme	catanionic	476:485	arg1	mixture					487:493	their catanionic mixture	470:493	their catanionic mixture	470:493	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	8	62	dep	cells	2164:2168	arg1	related					2170:2176	related	2170:2176	related	2170:2176	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	8	63	used	utilized	1913:1920	arg2	protocol					1897:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	8	64	theme	surfactant	1856:1865	arg1	protocol					1897:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	0	65	theme	bovine	61:66	arg1	albumin					74:80	bovine serum albumin	61:80	bovine serum albumin assisted by cationic and anionic surfactants	61:125	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	2	66	theme	biophysical	645:655	arg1	techniques					657:666	biophysical techniques	645:666	biophysical techniques	645:666	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	3	67	from	presence	903:910	arg1	refolding					871:879	minimum refolding	863:879	minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	863:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	2	68	theme	cationic	772:779	arg1	surfactants					790:800	cationic, anionic surfactants	772:800	cationic, anionic surfactants	772:800	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	8	69	theme	protein	1929:1935	arg1	studies					1960:1966	the protein renaturation/refolding studies	1925:1966	the protein renaturation/refolding studies	1925:1966	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	5	70	theme	mixture	1419:1425	arg1	compositions					1427:1438	their mixture compositions	1413:1438	their mixture compositions	1413:1438	On other hand, CTAB and SDS are more effective and reflect a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions.					
31520999	7	71	theme	CTAB	1648:1651	arg1	surfactant					1653:1662	CTAB surfactant	1648:1662	CTAB surfactant	1648:1662	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	7	72	theme	negative	1774:1781	arg1	charge					1783:1788	negative charge	1774:1788	negative charge on their surface	1774:1805	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	3	73	from	refolding	871:879	arg1	presence					903:910	the presence	899:910	the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	899:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	2	74	theme	catanionic	812:821	arg1	mixture					823:829	their catanionic mixture	806:829	their catanionic mixture	806:829	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	2	75	from	times	735:739	arg1	presence					760:767	the presence	756:767	the presence of cationic, anionic surfactants	756:800	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	0	76	theme	chaperone	142:150	arg1	protocol					152:159	artificial chaperone protocol	131:159	artificial chaperone protocol	131:159	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	7	77	theme	tail	1640:1643	arg1	presence					1584:1591	the presence	1580:1591	the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface	1580:1805	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	2	78	from	dilution	744:751	arg1	presence					760:767	the presence	756:767	the presence of cationic, anionic surfactants	756:800	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	7	79	theme	hydrophobic	1718:1728	arg1	interactions					1730:1741	electrostatic and hydrophobic interactions	1700:1741	electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface	1700:1805	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	1	80	theme	anionic	417:423	arg1	SDS					461:463	SDS	461:463	SDS	461:463	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	80	theme	anionic	417:423	arg1	sulphate					451:458	anionic surfactant sodium dodecyl sulphate	417:458	anionic surfactant sodium dodecyl sulphate (SDS)	417:464	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	8	81	theme	associated	1991:2000	arg1	problems					2002:2009	the associated problems	1987:2009	the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation	1987:2232	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	1	82	theme	sodium	436:441	arg1	SDS					461:463	SDS	461:463	SDS	461:463	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	82	theme	sodium	436:441	arg1	sulphate					451:458	anionic surfactant sodium dodecyl sulphate	417:458	anionic surfactant sodium dodecyl sulphate (SDS)	417:464	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	3	83	theme	diluted	884:890	arg1	BSA					892:894	diluted BSA	884:894	diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	884:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	7	84	theme	head	1603:1606	arg1	group					1608:1612	charge head group	1596:1612	charge head group	1596:1612	These results ascribed the presence of charge head group and length of hydrophobic tail of CTAB surfactant that plays an important task during electrostatic and hydrophobic interactions at pH 7.4 at which BSA carries negative charge on their surface.					
31520999	0	85	theme	assisted	82:89	arg1	albumin					74:80	bovine serum albumin	61:80	bovine serum albumin assisted by cationic and anionic surfactants	61:125	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	1	86	theme	sulphate	451:458	arg1	presence					346:353	the presence	342:353	the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	342:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	4	87	theme	refolding	1172:1180	arg1	process					1182:1188	refolding process	1172:1188	refolding process in the presence of methyl-β-cyclodextrin	1172:1229	Other mixtures (CTAB/SDS = 20/80 and 80/20) slightly played an effective role during refolding process in the presence of methyl-β-cyclodextrin.					
31520999	5	88	theme	other	1235:1239	arg1	hand					1241:1244	other hand	1235:1244	other hand	1235:1244	On other hand, CTAB and SDS are more effective and reflect a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions.					
31520999	0	89	theme	cationic	94:101	arg1	surfactants					115:125	cationic and anionic surfactants	94:125	cationic and anionic surfactants	94:125	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	3	90	theme	microstructure	1051:1064	arg1	formation					1076:1084	the unordered microstructure aggregate formation	1037:1084	the unordered microstructure aggregate formation	1037:1084	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	8	91	theme	renaturation/refolding	1937:1958	arg1	studies					1960:1966	the protein renaturation/refolding studies	1925:1966	the protein renaturation/refolding studies	1925:1966	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	2	92	theme	refolding	688:696	arg1	mechanism					698:706	the refolding mechanism	684:706	the refolding mechanism of denatured BSA	684:723	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	0	93	theme	anionic	107:113	arg1	surfactants					115:125	cationic and anionic surfactants	94:125	cationic and anionic surfactants	94:125	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	1	94	theme	cationic	358:365	arg1	CTAB					410:413	CTAB	410:413	CTAB	410:413	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	94	theme	cationic	358:365	arg1	bromide					401:407	cationic surfactant cetyltrimethylammonium bromide	358:407	cationic surfactant cetyltrimethylammonium bromide (CTAB)	358:414	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	8	95	used	used	2124:2127	arg2	aides					2099:2103	efficient and inexpensive folding aides	2065:2103	efficient and inexpensive folding aides	2065:2103	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	2	96	from	mixture	823:829	arg1	presence					760:767	the presence	756:767	the presence of cationic, anionic surfactants	756:800	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	1	97	theme	physiological	547:559	arg1	pH 7.4					561:566	physiological pH 7.4	547:566	physiological pH 7.4	547:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	98	theme	cetyltrimethylammonium	378:399	arg1	CTAB					410:413	CTAB	410:413	CTAB	410:413	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	98	theme	cetyltrimethylammonium	378:399	arg1	bromide					401:407	cationic surfactant cetyltrimethylammonium bromide	358:407	cationic surfactant cetyltrimethylammonium bromide (CTAB)	358:414	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	3	99	theme	mixture	928:934	arg1	presence					903:910	the presence	899:910	the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	899:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	4	100	theme	methyl-β-cyclodextrin	1209:1229	arg1	presence					1197:1204	the presence	1193:1204	the presence of methyl-β-cyclodextrin	1193:1229	Other mixtures (CTAB/SDS = 20/80 and 80/20) slightly played an effective role during refolding process in the presence of methyl-β-cyclodextrin.					
31520999	5	101	theme	solution	1337:1344	arg1	tendency					1311:1318	a good renaturation tendency	1291:1318	a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions	1291:1438	On other hand, CTAB and SDS are more effective and reflect a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions.					
31520999	8	102	theme	CTAB	1851:1854	arg1	protocol					1897:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	3	103	theme	1:1	915:917	arg1	mixture					928:934	1:1 rational mixture	915:934	1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	915:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	5	104	theme	good	1293:1296	arg1	tendency					1311:1318	a good renaturation tendency	1291:1318	a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions	1291:1438	On other hand, CTAB and SDS are more effective and reflect a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions.					
31520999	0	105	theme	guanidinium	25:35	arg1	hydrochloride					37:49	guanidinium hydrochloride	25:49	guanidinium hydrochloride	25:49	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	1	106	theme	bovine	312:317	arg1	BSA					334:336	BSA	334:336	BSA	334:336	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	106	theme	bovine	312:317	arg1	albumin					325:331	bovine serum albumin	312:331	guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA)	267:337	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	8	107	theme	assisted	1867:1874	arg1	protocol					1897:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	CTAB surfactant assisted artificial chaperone protocol	1851:1904	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	3	108	theme	SDS	948:950	arg1	mixture					928:934	1:1 rational mixture	915:934	1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	915:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	1	109	theme	two-step	605:612	arg1	method					614:619	artificial chaperone-assisted two-step method	575:619	artificial chaperone-assisted two-step method	575:619	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	1	110	theme	albumin	325:331	arg1	behaviour					254:262	the cooperative refolding/renaturation behaviour	215:262	the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	215:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	3	111	theme	CTAB	939:942	arg1	mixture					928:934	1:1 rational mixture	915:934	1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it	915:973	We have found that minimum refolding of diluted BSA in the presence of 1:1 rational mixture of CTAB and SDS (CTAB/SDS = 50/50), it may be due to the micelles formation which is responsible for the unordered microstructure aggregate formation.					
31520999	5	112	theme	methyl-β-cyclodextrin	1377:1397	arg1	existence					1364:1372	existence	1364:1372	existence of methyl-β-cyclodextrin as compare to their mixture compositions	1364:1438	On other hand, CTAB and SDS are more effective and reflect a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions.					
31520999	0	113	theme	serum	68:72	arg1	albumin					74:80	bovine serum albumin	61:80	bovine serum albumin assisted by cationic and anionic surfactants	61:125	Comparative refolding of guanidinium hydrochloride denatured bovine serum albumin assisted by cationic and anionic surfactants via artificial chaperone protocol: Biophysical insight.					
31520999	6	114	contain	has	1459:1461	arg2	tendency					1487:1494	the better renaturation tendency	1463:1494	the better renaturation tendency	1463:1494	But overall, CTAB has the better renaturation tendency as compare to SDS in the existence of methyl-β-cyclodextrin.					
31520999	6	114	contain	has	1459:1461	arg1	CTAB					1454:1457	CTAB	1454:1457	CTAB	1454:1457	But overall, CTAB has the better renaturation tendency as compare to SDS in the existence of methyl-β-cyclodextrin.					
31520999	5	115	theme	denatured	1323:1331	arg1	solution					1337:1344	denatured BSA solution	1323:1344	denatured BSA solution	1323:1344	On other hand, CTAB and SDS are more effective and reflect a good renaturation tendency of denatured BSA solution separately and in existence of methyl-β-cyclodextrin as compare to their mixture compositions.					
31520999	2	116	theme	BSA	721:723	arg1	mechanism					698:706	the refolding mechanism	684:706	the refolding mechanism of denatured BSA	684:723	Here, we have employed biophysical techniques to characterize the refolding mechanism of denatured BSA after 200 times of dilution in the presence of cationic, anionic surfactants and their catanionic mixture, separately.					
31520999	1	117	theme	sodium	520:525	arg1	buffer					537:542	60 mM sodium phosphate buffer	514:542	60 mM sodium phosphate buffer	514:542	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	8	118	theme	protein	2203:2209	arg1	misfolding/aggregation					2211:2232	protein misfolding/aggregation	2203:2232	protein misfolding/aggregation	2203:2232	These biophysical parameters suggest that, CTAB surfactant assisted artificial chaperone protocol may be utilized in the protein renaturation/refolding studies, which may address the associated problems of biotechnological industries for the development of efficient and inexpensive folding aides, which may also be used to produced genetically engineered cells related diseases, resulting from protein misfolding/aggregation.					
31520999	4	119	theme	Other	1087:1091	arg1	mixtures					1093:1100	Other mixtures	1087:1100	Other mixtures (CTAB/SDS = 20/80 and 80/20)	1087:1129	Other mixtures (CTAB/SDS = 20/80 and 80/20) slightly played an effective role during refolding process in the presence of methyl-β-cyclodextrin.					
31520999	1	120	theme	buffer	537:542	arg1	solution					502:509	the solution	498:509	the solution of 60 mM sodium phosphate buffer of physiological pH 7.4	498:566	In the present study, we report the cooperative refolding/renaturation behaviour of guanidinium hydrochloride (GdnHCl) denatured bovine serum albumin (BSA) in the presence of cationic surfactant cetyltrimethylammonium bromide (CTAB), anionic surfactant sodium dodecyl sulphate (SDS) and their catanionic mixture in the solution of 60 mM sodium phosphate buffer of physiological pH 7.4, using artificial chaperone-assisted two-step method.					
31520999	6	121	theme	renaturation	1474:1485	arg1	tendency					1487:1494	the better renaturation tendency	1463:1494	the better renaturation tendency	1463:1494	But overall, CTAB has the better renaturation tendency as compare to SDS in the existence of methyl-β-cyclodextrin.					
30172411	6	0	theme	24	1287:1288	arg1	h					1290:1290	h	1290:1290	h	1290:1290	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	0	1	theme	mastitis	200:207	arg1	severity					171:178	severity	171:178	control of bacterial growth but not severity of Escherichia coli mastitis	135:207	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	5	2	dep	responders	903:912	arg1	HR					915:916	HR	915:916	HR	915:916	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	5	2	dep	responders	903:912	arg1	responders					903:912	high and low responders	890:912	high and low responders (HR and LR, respectively)	890:938	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	5	2	dep	responders	903:912	arg1	LR					922:923	LR	922:923	LR	922:923	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	9	3	theme	current	1488:1494	arg1	study					1496:1500	The current study	1484:1500	The current study	1484:1500	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	0	4	theme	cytokine	90:97	arg1	production					99:108	lipopolysaccharide-induced cytokine production	63:108	lipopolysaccharide-induced cytokine production	63:108	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	8	5	from	differences	1393:1403	arg1	concentrations					1442:1455	concentrations	1442:1455	concentrations of milk BSA	1442:1467	No differences in post-infection milk production or concentrations of milk BSA were measured.					
30172411	8	5	from	differences	1393:1403	arg1	production					1428:1437	post-infection milk production	1408:1437	post-infection milk production	1408:1437	No differences in post-infection milk production or concentrations of milk BSA were measured.					
30172411	9	6	theme	innate	1564:1569	arg1	response					1571:1578	their innate response	1558:1578	their innate response	1558:1578	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	6	7	theme	somatic	1187:1193	arg1	count					1200:1204	an elevated somatic cell count	1175:1204	an elevated somatic cell count	1175:1204	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	8	8	theme	milk	1423:1426	arg1	production					1428:1437	post-infection milk production	1408:1437	post-infection milk production	1408:1437	No differences in post-infection milk production or concentrations of milk BSA were measured.					
30172411	2	9	theme	mammary	525:531	arg1	gland					533:537	the mammary gland	521:537	the mammary gland	521:537	An effective innate immune response by the host is key to controlling infection, but it should also limit collateral damage to the mammary gland.					
30172411	0	10	from	Variation	0:8	arg1	expression					24:33	fibroblast expression	13:33	fibroblast expression of toll-like receptor 4	13:57	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	0	10	from	Variation	0:8	arg1	production					99:108	lipopolysaccharide-induced cytokine production	63:108	lipopolysaccharide-induced cytokine production	63:108	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	4	11	theme	protein	852:858	arg1	production					860:869	lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production	805:869	production	860:869	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	3	12	theme	Between-animal	540:553	arg1	differences					555:565	Between-animal differences	540:565	Between-animal differences in mastitis severity	540:586	Between-animal differences in mastitis severity have been attributed to variability in the innate response.					
30172411	3	13	from	differences	555:565	arg1	severity					579:586	mastitis severity	570:586	mastitis severity	570:586	Between-animal differences in mastitis severity have been attributed to variability in the innate response.					
30172411	6	14	theme	milk	1274:1277	arg1	IL-8					1279:1282	milk IL-8	1274:1282	milk IL-8	1274:1282	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	4	15	theme	IL-6	847:850	arg1	production					860:869	lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production	805:869	production	860:869	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	10	16	theme	dermal	1733:1738	arg1	fibroblast					1740:1749	The dermal fibroblast	1729:1749	The dermal fibroblast	1729:1749	The dermal fibroblast may be used in conjunction with other cell types to determine how the innate response is regulated to mitigate unnecessary injury to the mammary gland while still effectively clearing the pathogen.					
30172411	9	17	from	upregulation	1542:1553	arg1	response					1571:1578	their innate response	1558:1578	their innate response	1558:1578	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	9	18	theme	exuberant	1692:1700	arg1	response					1702:1709	an exuberant response	1689:1709	an exuberant response to the infection	1689:1726	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	7	19	theme	bacterial	1372:1380	arg1	growth					1382:1387	bacterial growth	1372:1387	bacterial growth	1372:1387	The HR animals were also significantly more capable of limiting bacterial growth.					
30172411	6	20	theme	elevated	1178:1185	arg1	count					1200:1204	an elevated somatic cell count	1175:1204	an elevated somatic cell count	1175:1204	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	4	21	theme	toll-like	768:776	arg1	receptor					778:785	the toll-like receptor	764:785	the toll-like receptor 4 gene (TLR4)	764:799	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	2	22	theme	immune	414:419	arg1	key					445:447	key	445:447	key	445:447	An effective innate immune response by the host is key to controlling infection, but it should also limit collateral damage to the mammary gland.					
30172411	2	22	theme	immune	414:419	arg1	response					421:428	An effective innate immune response	394:428	An effective innate immune response by the host	394:440	An effective innate immune response by the host is key to controlling infection, but it should also limit collateral damage to the mammary gland.					
30172411	5	23	from	difference	1006:1015	arg1	rank					1020:1023	rank	1020:1023	rank	1020:1023	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	8	24	theme	milk	1460:1463	arg1	BSA					1465:1467	milk BSA	1460:1467	milk BSA	1460:1467	No differences in post-infection milk production or concentrations of milk BSA were measured.					
30172411	2	25	theme	innate	407:412	arg1	key					445:447	key	445:447	key	445:447	An effective innate immune response by the host is key to controlling infection, but it should also limit collateral damage to the mammary gland.					
30172411	2	25	theme	innate	407:412	arg1	response					421:428	An effective innate immune response	394:428	An effective innate immune response by the host	394:440	An effective innate immune response by the host is key to controlling infection, but it should also limit collateral damage to the mammary gland.					
30172411	9	26	contain	have	1528:1531	arg1	animals					1520:1526	HR animals	1517:1526	HR animals	1517:1526	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	9	26	contain	have	1528:1531	arg2	beneficial					1588:1597	beneficial	1588:1597	beneficial	1588:1597	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	9	26	contain	have	1528:1531	arg2	upregulation					1542:1553	an early upregulation	1533:1553	an early upregulation in their innate response that is beneficial for bacterial clearance	1533:1621	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	10	27	theme	innate	1821:1826	arg1	response					1828:1835	the innate response	1817:1835	the innate response	1817:1835	The dermal fibroblast may be used in conjunction with other cell types to determine how the innate response is regulated to mitigate unnecessary injury to the mammary gland while still effectively clearing the pathogen.					
30172411	4	28	theme	-induced	829:836	arg1	IL-8					838:841	lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production	805:869	IL-8	838:841	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	0	29	theme	lipopolysaccharide-induced	63:88	arg1	production					99:108	lipopolysaccharide-induced cytokine production	63:108	lipopolysaccharide-induced cytokine production	63:108	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	7	30	theme	HR	1312:1313	arg1	capable					1352:1358	capable	1352:1358	capable	1352:1358	The HR animals were also significantly more capable of limiting bacterial growth.					
30172411	7	30	theme	HR	1312:1313	arg1	animals					1315:1321	The HR animals	1308:1321	The HR animals	1308:1321	The HR animals were also significantly more capable of limiting bacterial growth.					
30172411	2	31	theme	effective	397:405	arg1	key					445:447	key	445:447	key	445:447	An effective innate immune response by the host is key to controlling infection, but it should also limit collateral damage to the mammary gland.					
30172411	2	31	theme	effective	397:405	arg1	response					421:428	An effective innate immune response	394:428	An effective innate immune response by the host	394:440	An effective innate immune response by the host is key to controlling infection, but it should also limit collateral damage to the mammary gland.					
30172411	6	32	from	post-infection	1233:1246	arg1	production					1260:1269	greater production	1252:1269	greater production of milk IL-8	1252:1282	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	6	32	from	post-infection	1233:1246	arg1	count					1200:1204	an elevated somatic cell count	1175:1204	an elevated somatic cell count	1175:1204	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	6	32	from	post-infection	1233:1246	arg1	response					1216:1223	fever response	1210:1223	fever response	1210:1223	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	9	33	theme	early	1536:1540	arg1	beneficial					1588:1597	beneficial	1588:1597	beneficial	1588:1597	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	9	33	theme	early	1536:1540	arg1	upregulation					1542:1553	an early upregulation	1533:1553	an early upregulation in their innate response that is beneficial for bacterial clearance	1533:1621	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	6	34	theme	acute	1085:1089	arg1	response					1091:1098	an acute response	1082:1098	an acute response to the infection with varying degrees in severity	1082:1148	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	10	35	theme	mammary	1888:1894	arg1	gland					1896:1900	the mammary gland	1884:1900	the mammary gland	1884:1900	The dermal fibroblast may be used in conjunction with other cell types to determine how the innate response is regulated to mitigate unnecessary injury to the mammary gland while still effectively clearing the pathogen.					
30172411	1	36	theme	dairy	307:311	arg1	industry					313:320	the dairy industry	303:320	the dairy industry	303:320	Mastitis caused by environmental pathogens such as Escherichia coli is highly problematic to the dairy industry because it incurs substantial cost and tends to be difficult to manage.					
30172411	0	37	theme	bacterial	146:154	arg1	growth					156:161	bacterial growth	146:161	bacterial growth	146:161	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	4	38	theme	composite	740:748	arg1	expression					750:759	composite expression	740:759	composite expression of the toll-like receptor 4 gene (TLR4)	740:799	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	6	39	contain	had	1171:1173	arg2	production					1260:1269	greater production	1252:1269	greater production of milk IL-8	1252:1282	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	6	39	contain	had	1171:1173	arg1	animals					1163:1169	HR animals	1160:1169	HR animals	1160:1169	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	6	39	contain	had	1171:1173	arg2	count					1200:1204	an elevated somatic cell count	1175:1204	an elevated somatic cell count	1175:1204	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	6	39	contain	had	1171:1173	arg2	response					1216:1223	fever response	1210:1223	fever response	1210:1223	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	0	40	theme	fibroblast	13:22	arg1	expression					24:33	fibroblast expression	13:33	fibroblast expression of toll-like receptor 4	13:57	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	3	41	theme	mastitis	570:577	arg1	severity					579:586	mastitis severity	570:586	mastitis severity	570:586	Between-animal differences in mastitis severity have been attributed to variability in the innate response.					
30172411	0	42	theme	toll-like	38:46	arg1	receptor					48:55	toll-like receptor 4	38:57	toll-like receptor 4	38:57	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	6	43	with	infection	1107:1115	arg1	degrees					1130:1136	varying degrees	1122:1136	varying degrees in severity	1122:1148	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	9	44	theme	bacterial	1603:1611	arg1	clearance					1613:1621	bacterial clearance	1603:1621	bacterial clearance	1603:1621	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	4	45	theme	current	655:661	arg1	study					663:667	the current study	651:667	the current study	651:667	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	6	46	theme	HR	1160:1161	arg1	animals					1163:1169	HR animals	1160:1169	HR animals	1160:1169	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	1	47	theme	environmental	229:241	arg1	coli					273:276	Escherichia coli	261:276	Escherichia coli	261:276	Mastitis caused by environmental pathogens such as Escherichia coli is highly problematic to the dairy industry because it incurs substantial cost and tends to be difficult to manage.					
30172411	1	47	theme	environmental	229:241	arg1	pathogens					243:251	environmental pathogens	229:251	environmental pathogens such as Escherichia coli	229:276	Mastitis caused by environmental pathogens such as Escherichia coli is highly problematic to the dairy industry because it incurs substantial cost and tends to be difficult to manage.					
30172411	0	48	theme	growth	156:161	arg1	control					135:141	control	135:141	control of bacterial growth but not severity of Escherichia coli mastitis	135:207	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	5	49	theme	P4	968:969	arg1	strain					971:976	the P4 strain	964:976	the P4 strain of E. coli to determine how difference in rank would affect response to mastitis	964:1057	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	6	50	theme	IL-8	1279:1282	arg1	production					1260:1269	greater production	1252:1269	greater production of milk IL-8	1252:1282	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	6	50	theme	IL-8	1279:1282	arg1	count					1200:1204	an elevated somatic cell count	1175:1204	an elevated somatic cell count	1175:1204	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	6	50	theme	IL-8	1279:1282	arg1	response					1216:1223	fever response	1210:1223	fever response	1210:1223	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	6	51	theme	12	1228:1229	arg1	h					1231:1231	h	1231:1231	h	1231:1231	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	8	52	theme	BSA	1465:1467	arg1	concentrations					1442:1455	concentrations	1442:1455	concentrations of milk BSA	1442:1467	No differences in post-infection milk production or concentrations of milk BSA were measured.					
30172411	8	52	theme	BSA	1465:1467	arg1	production					1428:1437	post-infection milk production	1408:1437	post-infection milk production	1408:1437	No differences in post-infection milk production or concentrations of milk BSA were measured.					
30172411	4	53	used	used	673:676	arg2	we					670:671	we	670:671	we	670:671	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	0	54	dep	Escherichia	183:193	arg1	coli					195:198	coli	195:198	coli	195:198	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	0	55	theme	receptor	48:55	arg1	expression					24:33	fibroblast expression	13:33	fibroblast expression of toll-like receptor 4	13:57	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	0	55	theme	receptor	48:55	arg1	production					99:108	lipopolysaccharide-induced cytokine production	63:108	lipopolysaccharide-induced cytokine production	63:108	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	1	56	theme	substantial	340:350	arg1	cost					352:355	substantial cost	340:355	substantial cost	340:355	Mastitis caused by environmental pathogens such as Escherichia coli is highly problematic to the dairy industry because it incurs substantial cost and tends to be difficult to manage.					
30172411	6	57	theme	greater	1252:1258	arg1	production					1260:1269	greater production	1252:1269	greater production of milk IL-8	1252:1282	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	10	58	theme	cell	1789:1792	arg1	types					1794:1798	other cell types	1783:1798	other cell types	1783:1798	The dermal fibroblast may be used in conjunction with other cell types to determine how the innate response is regulated to mitigate unnecessary injury to the mammary gland while still effectively clearing the pathogen.					
30172411	5	59	theme	high	890:893	arg1	HR					915:916	HR	915:916	HR	915:916	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	5	59	theme	high	890:893	arg1	responders					903:912	high and low responders	890:912	high and low responders (HR and LR, respectively)	890:938	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	5	59	theme	high	890:893	arg1	LR					922:923	LR	922:923	LR	922:923	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	6	60	theme	varying	1122:1128	arg1	degrees					1130:1136	varying degrees	1122:1136	varying degrees in severity	1122:1148	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	0	61	theme	Escherichia	183:193	arg1	mastitis					200:207	Escherichia coli mastitis	183:207	Escherichia coli mastitis	183:207	Variation in fibroblast expression of toll-like receptor 4 and lipopolysaccharide-induced cytokine production between animals predicts control of bacterial growth but not severity of Escherichia coli mastitis.					
30172411	5	62	theme	coli	984:987	arg1	strain					971:976	the P4 strain	964:976	the P4 strain of E. coli to determine how difference in rank would affect response to mastitis	964:1057	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	3	63	theme	innate	631:636	arg1	response					638:645	the innate response	627:645	the innate response	627:645	Between-animal differences in mastitis severity have been attributed to variability in the innate response.					
30172411	9	64	theme	HR	1517:1518	arg1	animals					1520:1526	HR animals	1517:1526	HR animals	1517:1526	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	4	65	theme	rank	718:721	arg1	animals					723:729	rank animals	718:729	rank animals	718:729	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	6	66	theme	h	1231:1231	arg1	post-infection					1233:1246	12 h post-infection	1228:1246	12 h post-infection	1228:1246	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	6	67	theme	fever	1210:1214	arg1	response					1216:1223	fever response	1210:1223	fever response	1210:1223	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	10	68	theme	other	1783:1787	arg1	types					1794:1798	other cell types	1783:1798	other cell types	1783:1798	The dermal fibroblast may be used in conjunction with other cell types to determine how the innate response is regulated to mitigate unnecessary injury to the mammary gland while still effectively clearing the pathogen.					
30172411	4	69	theme	receptor	778:785	arg1	gene					789:792	the toll-like receptor 4 gene	764:792	the toll-like receptor 4 gene (TLR4)	764:799	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	4	69	theme	receptor	778:785	arg1	TLR4					795:798	TLR4	795:798	TLR4	795:798	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	5	70	theme	low	899:901	arg1	HR					915:916	HR	915:916	HR	915:916	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	5	70	theme	low	899:901	arg1	responders					903:912	high and low responders	890:912	high and low responders (HR and LR, respectively)	890:938	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	5	70	theme	low	899:901	arg1	LR					922:923	LR	922:923	LR	922:923	Animals ranked as high and low responders (HR and LR, respectively) were then infected with the P4 strain of E. coli to determine how difference in rank would affect response to mastitis.					
30172411	10	71	theme	unnecessary	1862:1872	arg1	injury					1874:1879	unnecessary injury	1862:1879	unnecessary injury	1862:1879	The dermal fibroblast may be used in conjunction with other cell types to determine how the innate response is regulated to mitigate unnecessary injury to the mammary gland while still effectively clearing the pathogen.					
30172411	10	72	with	conjunction	1766:1776	arg1	types					1794:1798	other cell types	1783:1798	other cell types	1783:1798	The dermal fibroblast may be used in conjunction with other cell types to determine how the innate response is regulated to mitigate unnecessary injury to the mammary gland while still effectively clearing the pathogen.					
30172411	2	73	theme	collateral	500:509	arg1	damage					511:516	collateral damage	500:516	collateral damage to the mammary gland	500:537	An effective innate immune response by the host is key to controlling infection, but it should also limit collateral damage to the mammary gland.					
30172411	9	74	theme	tissue	1665:1670	arg1	damage					1672:1677	tissue damage	1665:1677	tissue damage caused by an exuberant response to the infection	1665:1726	The current study indicates that HR animals have an early upregulation in their innate response that is beneficial for bacterial clearance; however, they are equally susceptible to tissue damage caused by an exuberant response to the infection.					
30172411	6	75	from	degrees	1130:1136	arg1	severity					1141:1148	severity	1141:1148	severity	1141:1148	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	4	76	theme	dermal	686:691	arg1	fibroblast					693:702	primary dermal fibroblast	678:702	primary dermal fibroblast	678:702	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	4	76	theme	dermal	686:691	arg1	model					709:713	a model	707:713	a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production	707:869	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	6	77	theme	h	1290:1290	arg1	post-infection					1292:1305	24 h post-infection	1287:1305	24 h post-infection	1287:1305	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	8	78	theme	post-infection	1408:1421	arg1	production					1428:1437	post-infection milk production	1408:1437	post-infection milk production	1408:1437	No differences in post-infection milk production or concentrations of milk BSA were measured.					
30172411	4	79	theme	gene	789:792	arg1	production					860:869	lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production	805:869	production	860:869	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	4	79	theme	gene	789:792	arg1	IL-8					838:841	lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production	805:869	IL-8	838:841	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	4	79	theme	gene	789:792	arg1	expression					750:759	composite expression	740:759	composite expression of the toll-like receptor 4 gene (TLR4)	740:799	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	4	80	theme	primary	678:684	arg1	fibroblast					693:702	primary dermal fibroblast	678:702	primary dermal fibroblast	678:702	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	4	80	theme	primary	678:684	arg1	model					709:713	a model	707:713	a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production	707:869	In the current study, we used primary dermal fibroblast as a model to rank animals based on composite expression of the toll-like receptor 4 gene (TLR4) and lipopolysaccharide (LPS)-induced IL-8 and IL-6 protein production.					
30172411	6	81	theme	cell	1195:1198	arg1	count					1200:1204	an elevated somatic cell count	1175:1204	an elevated somatic cell count	1175:1204	All animals developed an acute response to the infection with varying degrees in severity; however, HR animals had an elevated somatic cell count and fever response at 12 h post-infection and greater production of milk IL-8 at 24 h post-infection.					
30172411	10	82	used	used	1758:1761	arg2	fibroblast					1740:1749	The dermal fibroblast	1729:1749	The dermal fibroblast	1729:1749	The dermal fibroblast may be used in conjunction with other cell types to determine how the innate response is regulated to mitigate unnecessary injury to the mammary gland while still effectively clearing the pathogen.					
31076349	3	0	theme	fluorescence	687:698	arg1	signal					700:705	the fluorescence signal	683:705	the fluorescence signal	683:705	Thus, Endo-H activities could be evaluated easily and quantitatively by measuring the fluorescence signal.					
31076349	4	1	theme	synthesized	751:761	arg1	probe					779:783	a previously synthesized pentasaccharide probe	738:783	a previously synthesized pentasaccharide probe	738:783	Using both this probe (1) and a previously synthesized pentasaccharide probe, the hydrolysis activity of Endo-H and Endo-M were investigated.					
31076349	2	2	theme	residue	442:448	arg1	point					417:421	the branching point	403:421	the branching point of the β-mannoside residue	403:448	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	5	3	with	correlation	879:889	arg1	specificity					910:920	the substrate specificity	896:920	the substrate specificity of each enzyme	896:935	The results clearly showed a correlation with the substrate specificity of each enzyme.					
31076349	0	4	theme	Fluorogenic	0:10	arg1	probe					12:16	Fluorogenic probe	0:16	Fluorogenic probe for measuring high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	0:106	Fluorogenic probe for measuring high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity.					
31076349	1	5	theme	hydrolytic	209:218	arg1	activity					220:227	the hydrolytic activity	205:227	the hydrolytic activity of endo-β-N-acetylglucosaminidase	205:261	We synthesized a fluorogenic probe with a high-mannose type heptasaccharide structure to detect the hydrolytic activity of endo-β-N-acetylglucosaminidase from Streptomyces plicatus (Endo-H).					
31076349	3	6	theme	Endo-H	607:612	arg1	activities					614:623	Endo-H activities	607:623	Endo-H activities	607:623	Thus, Endo-H activities could be evaluated easily and quantitatively by measuring the fluorescence signal.					
31076349	1	7	with	probe	138:142	arg1	structure					185:193	a high-mannose type heptasaccharide structure	149:193	a high-mannose type heptasaccharide structure	149:193	We synthesized a fluorogenic probe with a high-mannose type heptasaccharide structure to detect the hydrolytic activity of endo-β-N-acetylglucosaminidase from Streptomyces plicatus (Endo-H).					
31076349	2	8	from	point	417:421	arg1	group					472:476	2,4-dinitrophenyl group	454:476	2,4-dinitrophenyl group	454:476	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	2	8	from	point	417:421	arg1	group					376:380	an N-methylanthraniloyl group	352:380	an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue	352:448	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	2	9	theme	N-methylanthraniloyl	355:374	arg1	group					376:380	an N-methylanthraniloyl group	352:380	an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue	352:448	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	2	10	theme	quencher	483:490	arg1	molecule					492:499	a quencher molecule	481:499	a quencher molecule	481:499	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	2	10	theme	quencher	483:490	arg1	derivative					320:329	The heptasaccharide derivative	300:329	The heptasaccharide derivative (1)	300:333	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	1	11	theme	fluorogenic	126:136	arg1	probe					138:142	a fluorogenic probe	124:142	a fluorogenic probe with a high-mannose type heptasaccharide structure to detect the hydrolytic activity of endo-β-N-acetylglucosaminidase from Streptomyces plicatus (Endo-H)	124:297	We synthesized a fluorogenic probe with a high-mannose type heptasaccharide structure to detect the hydrolytic activity of endo-β-N-acetylglucosaminidase from Streptomyces plicatus (Endo-H).					
31076349	1	12	theme	endo-β-N-acetylglucosaminidase	232:261	arg1	activity					220:227	the hydrolytic activity	205:227	the hydrolytic activity of endo-β-N-acetylglucosaminidase	205:261	We synthesized a fluorogenic probe with a high-mannose type heptasaccharide structure to detect the hydrolytic activity of endo-β-N-acetylglucosaminidase from Streptomyces plicatus (Endo-H).					
31076349	5	13	theme	enzyme	930:935	arg1	specificity					910:920	the substrate specificity	896:920	the substrate specificity of each enzyme	896:935	The results clearly showed a correlation with the substrate specificity of each enzyme.					
31076349	0	14	theme	type	45:48	arg1	activity					99:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	Fluorogenic probe for measuring high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity.					
31076349	2	15	theme	fluorescence	577:588	arg1	intensity					590:598	increased fluorescence intensity	567:598	increased fluorescence intensity	567:598	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	2	16	theme	2,4-dinitrophenyl	454:470	arg1	group					472:476	2,4-dinitrophenyl group	454:476	2,4-dinitrophenyl group	454:476	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	0	17	theme	high-mannose	32:43	arg1	activity					99:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	Fluorogenic probe for measuring high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity.					
31076349	2	18	theme	increased	567:575	arg1	intensity					590:598	increased fluorescence intensity	567:598	increased fluorescence intensity	567:598	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	2	19	theme	reducing	508:515	arg1	end					517:519	the reducing end	504:519	the reducing end	504:519	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	0	20	theme	H	97:97	arg1	activity					99:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	Fluorogenic probe for measuring high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity.					
31076349	0	21	theme	glycan-specific	50:64	arg1	activity					99:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	Fluorogenic probe for measuring high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity.					
31076349	2	22	theme	reporter	387:394	arg1	dye					396:398	a reporter dye	385:398	a reporter dye	385:398	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	1	23	theme	high-mannose	151:162	arg1	structure					185:193	a high-mannose type heptasaccharide structure	149:193	a high-mannose type heptasaccharide structure	149:193	We synthesized a fluorogenic probe with a high-mannose type heptasaccharide structure to detect the hydrolytic activity of endo-β-N-acetylglucosaminidase from Streptomyces plicatus (Endo-H).					
31076349	4	24	theme	hydrolysis	790:799	arg1	activity					801:808	the hydrolysis activity	786:808	the hydrolysis activity of Endo-H and Endo-M	786:829	Using both this probe (1) and a previously synthesized pentasaccharide probe, the hydrolysis activity of Endo-H and Endo-M were investigated.					
31076349	4	25	theme	Endo-H	813:818	arg1	activity					801:808	the hydrolysis activity	786:808	the hydrolysis activity of Endo-H and Endo-M	786:829	Using both this probe (1) and a previously synthesized pentasaccharide probe, the hydrolysis activity of Endo-H and Endo-M were investigated.					
31076349	4	26	theme	Endo-M	824:829	arg1	activity					801:808	the hydrolysis activity	786:808	the hydrolysis activity of Endo-H and Endo-M	786:829	Using both this probe (1) and a previously synthesized pentasaccharide probe, the hydrolysis activity of Endo-H and Endo-M were investigated.					
31076349	5	27	theme	substrate	900:908	arg1	specificity					910:920	the substrate specificity	896:920	the substrate specificity of each enzyme	896:935	The results clearly showed a correlation with the substrate specificity of each enzyme.					
31076349	1	28	theme	type	164:167	arg1	structure					185:193	a high-mannose type heptasaccharide structure	149:193	a high-mannose type heptasaccharide structure	149:193	We synthesized a fluorogenic probe with a high-mannose type heptasaccharide structure to detect the hydrolytic activity of endo-β-N-acetylglucosaminidase from Streptomyces plicatus (Endo-H).					
31076349	4	29	theme	pentasaccharide	763:777	arg1	probe					779:783	a previously synthesized pentasaccharide probe	738:783	a previously synthesized pentasaccharide probe	738:783	Using both this probe (1) and a previously synthesized pentasaccharide probe, the hydrolysis activity of Endo-H and Endo-M were investigated.					
31076349	2	30	theme	heptasaccharide	304:318	arg1	molecule					492:499	a quencher molecule	481:499	a quencher molecule	481:499	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	2	30	theme	heptasaccharide	304:318	arg1	1					332:332	1	332:332	1	332:332	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	2	30	theme	heptasaccharide	304:318	arg1	derivative					320:329	The heptasaccharide derivative	300:329	The heptasaccharide derivative (1)	300:333	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	1	31	theme	heptasaccharide	169:183	arg1	structure					185:193	a high-mannose type heptasaccharide structure	149:193	a high-mannose type heptasaccharide structure	149:193	We synthesized a fluorogenic probe with a high-mannose type heptasaccharide structure to detect the hydrolytic activity of endo-β-N-acetylglucosaminidase from Streptomyces plicatus (Endo-H).					
31076349	2	32	theme	β-mannoside	430:440	arg1	residue					442:448	the β-mannoside residue	426:448	the β-mannoside residue	426:448	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31076349	0	33	theme	endo-β-N-acetylglucosaminidase	66:95	arg1	activity					99:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity	32:106	Fluorogenic probe for measuring high-mannose type glycan-specific endo-β-N-acetylglucosaminidase H activity.					
31076349	2	34	theme	branching	407:415	arg1	point					417:421	the branching point	403:421	the branching point of the β-mannoside residue	403:448	The heptasaccharide derivative (1) was labeled with an N-methylanthraniloyl group as a reporter dye at the branching point of the β-mannoside residue and 2,4-dinitrophenyl group as a quencher molecule at the reducing end, which was hydrolyzed by Endo-H, resulting in increased fluorescence intensity.					
31692384	7	0	theme	AL	1180:1181	arg1	ratio					1186:1190	AL:GL ratio	1180:1190	AL:GL ratio	1180:1190	Both drug content and AL:GL ratio appeared to affect water penetration into the gel matrix and drug release.					
31692384	2	1	dep	three-factor	445:456	arg1	centered					476:483	centered	476:483	centered	476:483	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	4	2	theme	numerical	762:770	arg1	optimization					772:783	numerical optimization	762:783	numerical optimization	762:783	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	10	3	theme	zero-order	1484:1493	arg1	model					1503:1507	zero-order release model	1484:1507	zero-order release model	1484:1507	In addition, increasing GA% caused deviation from zero-order release model.					
31692384	7	4	theme	drug	1163:1166	arg1	content					1168:1174	drug content	1163:1174	drug content	1163:1174	Both drug content and AL:GL ratio appeared to affect water penetration into the gel matrix and drug release.					
31692384	2	5	theme	studied	330:336	arg1	factors					338:344	the studied factors	326:344	the studied factors	326:344	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	1	6	theme	experiments	240:250	arg1	design					230:235	design	230:235	design of experiments (DOE)	230:256	Objective: The aim of this study was to optimize the formulation of alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE).					
31692384	4	7	theme	rate	830:833	arg1	studies.Results					835:849	DSC, FT-IR, SEM and release rate studies.Results	802:849	DSC, FT-IR, SEM and release rate studies.Results	802:849	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	11	8	theme	close	1568:1572	arg1	agreement					1574:1582	close agreement	1568:1582	close agreement with the predicted ones.Conclusion	1568:1617	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	3	9	theme	content	525:531	arg1	effects					510:516	The effects	506:516	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate	506:701	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	8	10	theme	GLZ	1331:1333	arg1	release					1335:1341	GLZ release	1331:1341	GLZ release	1331:1341	Generally, there was a direct relationship between GA% (X3) and GLZ release in pH 1.2 (Q0.5h and Q2h).					
31692384	3	11	dep	concentration	590:602	arg1	X3					626:627	X3	626:627	X3	626:627	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	3	11	dep	concentration	590:602	arg1	glutaraldehyde					605:618	glutaraldehyde	605:618	glutaraldehyde	605:618	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	3	11	dep	concentration	590:602	arg1	%					623:623	GA%	621:623	GA%	621:623	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	4	12	theme	release	822:828	arg1	rate					830:833	release rate	822:833	release rate	822:833	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	8	13	dep	was	1284:1286	arg1	Q2h					1364:1366	Q2h	1364:1366	Q2h	1364:1366	Generally, there was a direct relationship between GA% (X3) and GLZ release in pH 1.2 (Q0.5h and Q2h).					
31692384	8	13	dep	was	1284:1286	arg1	Q0.5h					1354:1358	Q0.5h	1354:1358	Q0.5h	1354:1358	Generally, there was a direct relationship between GA% (X3) and GLZ release in pH 1.2 (Q0.5h and Q2h).					
31692384	5	14	from	content	993:999	arg1	AL					1004:1005	AL	1004:1005	AL	1004:1005	On the other hand, increasing alginate content in AL:GL ratio (X2) increased IE (Y1).					
31692384	11	15	theme	predicted	1593:1601	arg1	ones.Conclusion					1603:1617	the predicted ones.Conclusion	1589:1617	the predicted ones.Conclusion	1589:1617	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	8	16	theme	direct	1290:1295	arg1	relationship					1297:1308	a direct relationship	1288:1308	a direct relationship between GA% (X3) and GLZ release	1288:1341	Generally, there was a direct relationship between GA% (X3) and GLZ release in pH 1.2 (Q0.5h and Q2h).					
31692384	11	17	theme	selected	1624:1631	arg1	useful					1698:1703	useful	1698:1703	useful	1698:1703	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	11	17	theme	selected	1624:1631	arg1	factors					1633:1639	The selected factors	1620:1639	The selected factors	1620:1639	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	6	18	theme	GLZ	1054:1056	arg1	GLZ					1054:1056	GLZ	1054:1056	GLZ	1054:1056	The amount of GLZ released Q0.5h, Q2h(pH 1.2) and Q4h(pH 7.4) decreased by increasing GLZ% (X1) and AL:GL ratio (X2).					
31692384	6	18	theme	GLZ	1054:1056	arg1	amount					1044:1049	The amount	1040:1049	The amount of GLZ released Q0.5h, Q2h(pH 1.2) and Q4h(pH 7.4)	1040:1100	The amount of GLZ released Q0.5h, Q2h(pH 1.2) and Q4h(pH 7.4) decreased by increasing GLZ% (X1) and AL:GL ratio (X2).					
31692384	3	19	from	effects	510:516	arg1	efficiency					670:679	incorporation efficiency	656:679	incorporation efficiency (IE)	656:684	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	3	19	from	effects	510:516	arg1	rate					698:701	release rate	690:701	release rate	690:701	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	3	19	from	effects	510:516	arg1	IE					682:683	IE	682:683	IE	682:683	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	1	20	theme	alginate-gelatin	161:176	arg1	beads					186:190	alginate-gelatin (AL-GL) beads	161:190	alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE)	161:256	Objective: The aim of this study was to optimize the formulation of alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE).					
31692384	9	21	theme	GLZ	1421:1423	arg1	release					1425:1431	GLZ release	1421:1431	GLZ release	1421:1431	However, in pH 7.4 (Q4h), increasing GA% decreased GLZ release.					
31692384	4	22	theme	optimized	721:729	arg1	formulation					731:741	The optimized formulation	717:741	The optimized formulation	717:741	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	7	23	theme	gel	1238:1240	arg1	matrix					1242:1247	gel matrix	1238:1247	gel matrix	1238:1247	Both drug content and AL:GL ratio appeared to affect water penetration into the gel matrix and drug release.					
31692384	3	24	theme	GLZ	521:523	arg1	content					525:531	GLZ content	521:531	GLZ content (GLZ%, X1)	521:542	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	2	25	theme	GLZ	369:371	arg1	pattern					381:387	GLZ release pattern	369:387	GLZ release pattern	369:387	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	11	26	theme	GLZ	1767:1769	arg1	pattern					1779:1785	GLZ release pattern	1767:1785	GLZ release pattern	1767:1785	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	11	27	theme	release	1771:1777	arg1	pattern					1779:1785	GLZ release pattern	1767:1785	GLZ release pattern	1767:1785	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	7	28	dep	matrix	1242:1247	arg1	the					1234:1236	the	1234:1236	the	1234:1236	Both drug content and AL:GL ratio appeared to affect water penetration into the gel matrix and drug release.					
31692384	11	29	theme	optimization	1673:1684	arg1	design					1686:1691	the optimization design	1669:1691	the optimization design	1669:1691	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	1	30	theme	AL-GL	179:183	arg1	beads					186:190	alginate-gelatin (AL-GL) beads	161:190	alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE)	161:256	Objective: The aim of this study was to optimize the formulation of alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE).					
31692384	2	31	theme	process.Methods	426:440	arg1	acceleration					393:404	acceleration	393:404	acceleration of the optimization process.Methods	393:440	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	2	31	theme	process.Methods	426:440	arg1	understanding					352:364	deep understanding	347:364	deep understanding of GLZ release pattern	347:387	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	2	31	theme	process.Methods	426:440	arg1	identification					284:297	identification	284:297	identification of the interaction between the studied factors	284:344	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	3	32	theme	interaction	641:651	arg1	effects					510:516	The effects	506:516	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate	506:701	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	7	33	theme	GL	1183:1184	arg1	ratio					1186:1190	AL:GL ratio	1180:1190	AL:GL ratio	1180:1190	Both drug content and AL:GL ratio appeared to affect water penetration into the gel matrix and drug release.					
31692384	11	34	theme	anticipated	1723:1733	arg1	characteristics					1747:1761	the anticipated formulation characteristics	1719:1761	the anticipated formulation characteristics	1719:1761	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	10	35	from	model	1503:1507	arg1	deviation					1469:1477	deviation	1469:1477	deviation from zero-order release model	1469:1507	In addition, increasing GA% caused deviation from zero-order release model.					
31692384	3	36	dep	ratio	553:557	arg1	X2					573:574	X2	573:574	X2	573:574	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	3	36	dep	ratio	553:557	arg1	AL					560:561	AL	560:561	AL	560:561	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	2	37	theme	optimization	413:424	arg1	process.Methods					426:440	the optimization process.Methods	409:440	the optimization process.Methods	409:440	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	5	38	theme	GL	1007:1008	arg1	X2					1017:1018	X2	1017:1018	X2	1017:1018	On the other hand, increasing alginate content in AL:GL ratio (X2) increased IE (Y1).					
31692384	5	38	theme	GL	1007:1008	arg1	ratio					1010:1014	GL ratio	1007:1014	GL ratio (X2)	1007:1019	On the other hand, increasing alginate content in AL:GL ratio (X2) increased IE (Y1).					
31692384	9	39	theme	increasing	1396:1405	arg1	%					1409:1409	increasing GA%	1396:1409	increasing GA%	1396:1409	However, in pH 7.4 (Q4h), increasing GA% decreased GLZ release.					
31692384	1	40	theme	beads	186:190	arg1	formulation					146:156	the formulation	142:156	the formulation of alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE)	142:256	Objective: The aim of this study was to optimize the formulation of alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE).					
31692384	0	41	theme	gliclazide	16:25	arg1	Optimization					0:11	Optimization	0:11	Optimization of gliclazide	0:25	Optimization of gliclazide loaded alginate-gelatin beads employing central composite design.					
31692384	6	42	theme	GL	1143:1144	arg1	X2					1153:1154	X2	1153:1154	X2	1153:1154	The amount of GLZ released Q0.5h, Q2h(pH 1.2) and Q4h(pH 7.4) decreased by increasing GLZ% (X1) and AL:GL ratio (X2).					
31692384	6	42	theme	GL	1143:1144	arg1	ratio					1146:1150	GL ratio	1143:1150	GL ratio (X2)	1143:1155	The amount of GLZ released Q0.5h, Q2h(pH 1.2) and Q4h(pH 7.4) decreased by increasing GLZ% (X1) and AL:GL ratio (X2).					
31692384	4	43	theme	FT-IR	807:811	arg1	studies.Results					835:849	DSC, FT-IR, SEM and release rate studies.Results	802:849	DSC, FT-IR, SEM and release rate studies.Results	802:849	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	7	44	theme	water	1211:1215	arg1	penetration					1217:1227	water penetration	1211:1227	water penetration into the gel matrix and drug release	1211:1264	Both drug content and AL:GL ratio appeared to affect water penetration into the gel matrix and drug release.					
31692384	3	45	dep	AL	560:561	arg1	ratio					566:570	GL ratio	563:570	GL ratio	563:570	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	11	46	with	agreement	1574:1582	arg1	ones.Conclusion					1603:1617	the predicted ones.Conclusion	1589:1617	the predicted ones.Conclusion	1589:1617	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	3	47	theme	polymer	545:551	arg1	ratio					553:557	polymer ratio	545:557	polymer ratio (AL:GL ratio, X2)	545:575	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	11	48	theme	formulation	1735:1745	arg1	characteristics					1747:1761	the anticipated formulation characteristics	1719:1761	the anticipated formulation characteristics	1719:1761	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	2	49	theme	release	373:379	arg1	pattern					381:387	GLZ release pattern	369:387	GLZ release pattern	369:387	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	5	50	theme	alginate	984:991	arg1	content					993:999	alginate content	984:999	alginate content in AL	984:1005	On the other hand, increasing alginate content in AL:GL ratio (X2) increased IE (Y1).					
31692384	0	51	theme	alginate-gelatin	34:49	arg1	beads					51:55	alginate-gelatin beads	34:55	alginate-gelatin beads employing central composite design	34:90	Optimization of gliclazide loaded alginate-gelatin beads employing central composite design.					
31692384	3	52	theme	ratio	553:557	arg1	effects					510:516	The effects	506:516	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate	506:701	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	2	53	theme	pattern	381:387	arg1	acceleration					393:404	acceleration	393:404	acceleration of the optimization process.Methods	393:440	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	2	53	theme	pattern	381:387	arg1	understanding					352:364	deep understanding	347:364	deep understanding of GLZ release pattern	347:387	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	2	53	theme	pattern	381:387	arg1	identification					284:297	identification	284:297	identification of the interaction between the studied factors	284:344	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	2	54	theme	three-factor	445:456	arg1	design					485:490	A three-factor, three-level face centered design	443:490	A three-factor, three-level face centered design	443:490	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	11	55	theme	actual	1514:1519	arg1	responses					1521:1529	The actual responses	1510:1529	The actual responses of the optimized formulation	1510:1558	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	0	56	theme	composite	75:83	arg1	design					85:90	central composite design	67:90	central composite design	67:90	Optimization of gliclazide loaded alginate-gelatin beads employing central composite design.					
31692384	4	57	theme	SEM	814:816	arg1	studies.Results					835:849	DSC, FT-IR, SEM and release rate studies.Results	802:849	DSC, FT-IR, SEM and release rate studies.Results	802:849	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	3	58	theme	incorporation	656:668	arg1	efficiency					670:679	incorporation efficiency	656:679	incorporation efficiency (IE)	656:684	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	3	58	theme	incorporation	656:668	arg1	IE					682:683	IE	682:683	IE	682:683	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	10	59	theme	release	1495:1501	arg1	model					1503:1507	zero-order release model	1484:1507	zero-order release model	1484:1507	In addition, increasing GA% caused deviation from zero-order release model.					
31692384	3	60	theme	concentration	590:602	arg1	effects					510:516	The effects	506:516	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate	506:701	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	4	61	theme	Increasing	852:861	arg1	X3					868:869	X3	868:869	X3	868:869	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	4	61	theme	Increasing	852:861	arg1	%					865:865	Increasing GA%	852:865	Increasing GA% (X3)	852:870	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	1	62	theme	study	120:124	arg1	aim					108:110	The aim	104:110	The aim of this study	104:124	Objective: The aim of this study was to optimize the formulation of alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE).					
31692384	11	63	theme	optimized	1538:1546	arg1	formulation					1548:1558	the optimized formulation	1534:1558	the optimized formulation	1534:1558	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	2	64	theme	interaction	306:316	arg1	acceleration					393:404	acceleration	393:404	acceleration of the optimization process.Methods	393:440	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	2	64	theme	interaction	306:316	arg1	understanding					352:364	deep understanding	347:364	deep understanding of GLZ release pattern	347:387	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	2	64	theme	interaction	306:316	arg1	identification					284:297	identification	284:297	identification of the interaction between the studied factors	284:344	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	4	65	theme	effect	920:925	arg1	magnitude					907:915	the highest magnitude	895:915	the highest magnitude of effect among the studied factors	895:951	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	3	66	theme	release	690:696	arg1	rate					698:701	release rate	690:701	release rate	690:701	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	4	67	theme	DSC	802:804	arg1	studies.Results					835:849	DSC, FT-IR, SEM and release rate studies.Results	802:849	DSC, FT-IR, SEM and release rate studies.Results	802:849	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	1	68	contain	containing	192:201	arg2	GLZ					215:217	GLZ	215:217	GLZ	215:217	Objective: The aim of this study was to optimize the formulation of alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE).					
31692384	1	68	contain	containing	192:201	arg2	gliclazide					203:212	gliclazide	203:212	gliclazide (GLZ) employing design of experiments (DOE)	203:256	Objective: The aim of this study was to optimize the formulation of alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE).					
31692384	1	68	contain	containing	192:201	arg1	beads					186:190	alginate-gelatin (AL-GL) beads	161:190	alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE)	161:256	Objective: The aim of this study was to optimize the formulation of alginate-gelatin (AL-GL) beads containing gliclazide (GLZ) employing design of experiments (DOE).					
31692384	3	69	theme	GL	563:564	arg1	ratio					566:570	GL ratio	563:570	GL ratio	563:570	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	4	70	theme	studied	937:943	arg1	factors					945:951	the studied factors	933:951	the studied factors	933:951	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	3	71	dep	content	525:531	arg1	%					537:537	GLZ%	534:537	GLZ%	534:537	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	3	71	dep	content	525:531	arg1	X1					540:541	X1	540:541	X1	540:541	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	4	72	theme	GA	863:864	arg1	X3					868:869	X3	868:869	X3	868:869	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	4	72	theme	GA	863:864	arg1	%					865:865	Increasing GA%	852:865	Increasing GA% (X3)	852:870	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	3	73	theme	crosslinker	578:588	arg1	concentration					590:602	crosslinker concentration	578:602	crosslinker concentration (glutaraldehyde, GA%, X3)	578:628	The effects of GLZ content (GLZ%, X1), polymer ratio (AL:GL ratio, X2), crosslinker concentration (glutaraldehyde, GA%, X3), and their interaction on incorporation efficiency (IE) and release rate were studied.					
31692384	11	74	theme	formulation	1548:1558	arg1	responses					1521:1529	The actual responses	1510:1529	The actual responses of the optimized formulation	1510:1558	The actual responses of the optimized formulation were in close agreement with the predicted ones.Conclusion: The selected factors and their levels studied in the optimization design were useful for tailoring the anticipated formulation characteristics and GLZ release pattern.					
31692384	2	75	theme	deep	347:350	arg1	understanding					352:364	deep understanding	347:364	deep understanding of GLZ release pattern	347:387	Significance: DOE enabled identification of the interaction between the studied factors, deep understanding of GLZ release pattern and acceleration of the optimization process.Methods: A three-factor, three-level face centered design was employed.					
31692384	0	76	theme	central	67:73	arg1	design					85:90	central composite design	67:90	central composite design	67:90	Optimization of gliclazide loaded alginate-gelatin beads employing central composite design.					
31692384	5	77	theme	other	961:965	arg1	hand					967:970	the other hand	957:970	the other hand	957:970	On the other hand, increasing alginate content in AL:GL ratio (X2) increased IE (Y1).					
31692384	6	78	dep	decreased	1102:1110	arg1	X2					1153:1154	X2	1153:1154	X2	1153:1154	The amount of GLZ released Q0.5h, Q2h(pH 1.2) and Q4h(pH 7.4) decreased by increasing GLZ% (X1) and AL:GL ratio (X2).					
31692384	6	78	dep	decreased	1102:1110	arg1	ratio					1146:1150	GL ratio	1143:1150	GL ratio (X2)	1143:1155	The amount of GLZ released Q0.5h, Q2h(pH 1.2) and Q4h(pH 7.4) decreased by increasing GLZ% (X1) and AL:GL ratio (X2).					
31692384	4	79	theme	highest	899:905	arg1	magnitude					907:915	the highest magnitude	895:915	the highest magnitude of effect among the studied factors	895:951	The optimized formulation was prepared using numerical optimization and evaluated by DSC, FT-IR, SEM and release rate studies.Results: Increasing GA% (X3) decreased IE (Y1) with the highest magnitude of effect among the studied factors.					
31692384	7	80	theme	drug	1253:1256	arg1	release					1258:1264	drug release	1253:1264	drug release	1253:1264	Both drug content and AL:GL ratio appeared to affect water penetration into the gel matrix and drug release.					
31283798	8	0	theme	family	1273:1278	arg1	Lachnospiraceae					1280:1294	the family Lachnospiraceae	1269:1294	the family Lachnospiraceae	1269:1294	In contrast, the family Lachnospiraceae and the genus Lactobacillus, both associated with healthy microbiota, increased in mice receiving scGOS/lcFOS diet.					
31283798	4	1	theme	beneficial	651:660	arg1	microorganisms					662:675	beneficial microorganisms	651:675	beneficial microorganisms	651:675	Prebiotics, defined as non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms, provide a relatively non-invasive approach to modulate the intestinal microbiota.					
31283798	9	2	theme	cell	1561:1564	arg1	subsets					1566:1572	the intestinal and systemic T helper cell subsets	1524:1572	the intestinal and systemic T helper cell subsets	1524:1572	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	10	3	theme	mineral	1705:1711	arg1	density					1713:1719	the bone mineral density	1696:1719	the bone mineral density in mice	1696:1727	As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks.					
31283798	8	4	theme	genus	1304:1308	arg1	both					1325:1328	both	1325:1328	both	1325:1328	In contrast, the family Lachnospiraceae and the genus Lactobacillus, both associated with healthy microbiota, increased in mice receiving scGOS/lcFOS diet.					
31283798	8	4	theme	genus	1304:1308	arg1	Lactobacillus					1310:1322	the genus Lactobacillus	1300:1322	the genus Lactobacillus	1300:1322	In contrast, the family Lachnospiraceae and the genus Lactobacillus, both associated with healthy microbiota, increased in mice receiving scGOS/lcFOS diet.					
31283798	7	5	theme	scGOS/lcFOS	1219:1229	arg1	supplementation					1239:1253	scGOS/lcFOS dietary supplementation	1219:1253	scGOS/lcFOS dietary supplementation	1219:1253	Interestingly, the genera Enterococcus and Clostridium were markedly decreased by scGOS/lcFOS dietary supplementation.					
31283798	6	6	with	supplementation	1036:1050	arg1	scGOS/lcFOS					1057:1067	scGOS/lcFOS	1057:1067	scGOS/lcFOS	1057:1067	We here show that dietary supplementation with scGOS/lcFOS has a pronounced effect on the composition of the fecal microbiota.					
31283798	0	7	theme	deficient	154:162	arg1	mice					164:167	IL-1 receptor antagonist deficient mice	129:167	IL-1 receptor antagonist deficient mice	129:167	Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.					
31283798	5	8	theme	experimental	978:989	arg1	arthritis					991:999	experimental arthritis	978:999	experimental arthritis in mice	978:1007	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	6	9	theme	pronounced	1075:1084	arg1	effect					1086:1091	a pronounced effect	1073:1091	a pronounced effect	1073:1091	We here show that dietary supplementation with scGOS/lcFOS has a pronounced effect on the composition of the fecal microbiota.					
31283798	5	10	theme	long-chain	926:935	arg1	fructo-oligosaccharides					937:959	10% long-chain fructo-oligosaccharides	922:959	10% long-chain fructo-oligosaccharides (scGOS/lcFOS)	922:973	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	5	10	theme	long-chain	926:935	arg1	scGOS/lcFOS					962:972	scGOS/lcFOS	962:972	scGOS/lcFOS	962:972	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	8	11	theme	healthy	1346:1352	arg1	microbiota					1354:1363	healthy microbiota	1346:1363	healthy microbiota	1346:1363	In contrast, the family Lachnospiraceae and the genus Lactobacillus, both associated with healthy microbiota, increased in mice receiving scGOS/lcFOS diet.					
31283798	2	12	theme	intestinal	405:414	arg1	microbiome					416:425	the intestinal microbiome	401:425	the intestinal microbiome	401:425	Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome.					
31283798	0	13	theme	receptor	134:141	arg1	antagonist					143:152	IL-1 receptor antagonist	129:152	IL-1 receptor antagonist deficient mice	129:167	Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.					
31283798	1	14	theme	new-onset	226:234	arg1	arthritis					272:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	The intestinal microbiome is perturbed in patients with new-onset and chronic autoimmune inflammatory arthritis.					
31283798	7	15	theme	genera	1156:1161	arg1	Enterococcus					1163:1174	the genera Enterococcus	1152:1174	the genera Enterococcus	1152:1174	Interestingly, the genera Enterococcus and Clostridium were markedly decreased by scGOS/lcFOS dietary supplementation.					
31283798	6	16	theme	dietary	1028:1034	arg1	supplementation					1036:1050	dietary supplementation	1028:1050	dietary supplementation with scGOS/lcFOS	1028:1067	We here show that dietary supplementation with scGOS/lcFOS has a pronounced effect on the composition of the fecal microbiota.					
31283798	11	17	theme	gut	1899:1901	arg1	microbiota					1903:1912	presumably healthy gut microbiota	1880:1912	presumably healthy gut microbiota	1880:1912	Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.					
31283798	5	18	theme	study	776:780	arg1	aim					764:766	The aim	760:766	The aim of this study	760:780	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	2	19	theme	Recent	283:288	arg1	studies					290:296	Recent studies	283:296	Recent studies in mouse models	283:312	Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome.					
31283798	6	20	theme	microbiota	1125:1134	arg1	composition					1100:1110	the composition	1096:1110	the composition of the fecal microbiota	1096:1134	We here show that dietary supplementation with scGOS/lcFOS has a pronounced effect on the composition of the fecal microbiota.					
31283798	5	21	with	supplementation	833:847	arg1	mixture					866:872	a prebiotic mixture	854:872	a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS)	854:973	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	0	22	theme	intestinal	72:81	arg1	microbiota					83:92	the intestinal microbiota	68:92	the intestinal microbiota	68:92	Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.					
31283798	5	23	theme	10	922:923	arg1	%					924:924	%	924:924	%	924:924	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	11	24	theme	bone	1926:1929	arg1	density					1939:1945	bone mineral density	1926:1945	bone mineral density	1926:1945	Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.					
31283798	5	25	theme	prebiotic	856:864	arg1	mixture					866:872	a prebiotic mixture	854:872	a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS)	854:973	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	9	26	from	effects	1513:1519	arg1	subsets					1566:1572	the intestinal and systemic T helper cell subsets	1524:1572	the intestinal and systemic T helper cell subsets	1524:1572	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	5	27	from	arthritis	991:999	arg1	mice					1004:1007	mice	1004:1007	mice	1004:1007	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	5	28	theme	dietary	825:831	arg1	supplementation					833:847	dietary supplementation	825:847	dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS)	825:973	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	3	29	theme	intestinal	457:466	arg1	microbiota					468:477	the intestinal microbiota	453:477	the intestinal microbiota	453:477	This makes modulation of the intestinal microbiota an interesting novel approach to suppress inflammatory arthritis.					
31283798	5	30	theme	galacto-oligosaccharides	893:916	arg1	mixture					866:872	a prebiotic mixture	854:872	a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS)	854:973	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	9	31	theme	microbiota	1475:1484	arg1	sufficient					1587:1596	sufficient	1587:1596	sufficient	1587:1596	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	9	31	theme	microbiota	1475:1484	arg1	alterations					1445:1455	the scGOS/lcFOS induced alterations	1421:1455	the scGOS/lcFOS induced alterations of the intestinal microbiota	1421:1484	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	5	32	theme	therapeutic	800:810	arg1	potential					812:820	the therapeutic potential	796:820	the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice	796:1007	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	9	33	dep	induced	1437:1443	arg1	scGOS/lcFOS					1425:1435	scGOS/lcFOS	1425:1435	scGOS/lcFOS	1425:1435	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	0	34	theme	diet	19:22	arg1	Supplementation					0:14	Supplementation	0:14	Supplementation of diet with non-digestible oligosaccharides	0:59	Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.					
31283798	2	35	from	studies	290:296	arg1	models					307:312	mouse models	301:312	mouse models	301:312	Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome.					
31283798	6	36	contain	has	1069:1071	arg2	effect					1086:1091	a pronounced effect	1073:1091	a pronounced effect	1073:1091	We here show that dietary supplementation with scGOS/lcFOS has a pronounced effect on the composition of the fecal microbiota.					
31283798	6	36	contain	has	1069:1071	arg1	supplementation					1036:1050	dietary supplementation	1028:1050	dietary supplementation with scGOS/lcFOS	1028:1067	We here show that dietary supplementation with scGOS/lcFOS has a pronounced effect on the composition of the fecal microbiota.					
31283798	3	37	theme	novel	494:498	arg1	approach					500:507	an interesting novel approach	479:507	an interesting novel approach to suppress inflammatory arthritis	479:542	This makes modulation of the intestinal microbiota an interesting novel approach to suppress inflammatory arthritis.					
31283798	0	38	theme	non-digestible	29:42	arg1	oligosaccharides					44:59	non-digestible oligosaccharides	29:59	non-digestible oligosaccharides	29:59	Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.					
31283798	9	39	theme	significant	1501:1511	arg1	effects					1513:1519	significant effects	1501:1519	significant effects on the intestinal and systemic T helper cell subsets	1501:1572	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	1	40	with	patients	212:219	arg1	arthritis					272:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	The intestinal microbiome is perturbed in patients with new-onset and chronic autoimmune inflammatory arthritis.					
31283798	11	41	theme	dietary	1825:1831	arg1	supplementation					1845:1859	dietary scGOS/lcFOS supplementation	1825:1859	dietary scGOS/lcFOS supplementation	1825:1859	Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.					
31283798	5	42	theme	%	879:879	arg1	galacto-oligosaccharides					893:916	90% short-chain galacto-oligosaccharides	877:916	90% short-chain galacto-oligosaccharides	877:916	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	0	43	with	Supplementation	0:14	arg1	oligosaccharides					44:59	non-digestible oligosaccharides	29:59	non-digestible oligosaccharides	29:59	Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.					
31283798	3	44	theme	inflammatory	521:532	arg1	arthritis					534:542	inflammatory arthritis	521:542	inflammatory arthritis	521:542	This makes modulation of the intestinal microbiota an interesting novel approach to suppress inflammatory arthritis.					
31283798	9	45	theme	intestinal	1528:1537	arg1	subsets					1566:1572	the intestinal and systemic T helper cell subsets	1524:1572	the intestinal and systemic T helper cell subsets	1524:1572	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	10	46	from	density	1713:1719	arg1	mice					1724:1727	mice	1724:1727	mice	1724:1727	As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks.					
31283798	9	47	theme	systemic	1543:1550	arg1	subsets					1566:1572	the intestinal and systemic T helper cell subsets	1524:1572	the intestinal and systemic T helper cell subsets	1524:1572	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	10	48	theme	significant	1672:1682	arg1	increase					1684:1691	a significant increase	1670:1691	a significant increase in the bone mineral density in mice	1670:1727	As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks.					
31283798	9	49	theme	helper	1554:1559	arg1	subsets					1566:1572	the intestinal and systemic T helper cell subsets	1524:1572	the intestinal and systemic T helper cell subsets	1524:1572	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	7	50	theme	dietary	1231:1237	arg1	supplementation					1239:1253	scGOS/lcFOS dietary supplementation	1219:1253	scGOS/lcFOS dietary supplementation	1219:1253	Interestingly, the genera Enterococcus and Clostridium were markedly decreased by scGOS/lcFOS dietary supplementation.					
31283798	4	51	theme	microorganisms	662:675	arg1	growth					628:633	growth	628:633	growth	628:633	Prebiotics, defined as non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms, provide a relatively non-invasive approach to modulate the intestinal microbiota.					
31283798	4	51	theme	microorganisms	662:675	arg1	activity					639:646	activity	639:646	activity	639:646	Prebiotics, defined as non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms, provide a relatively non-invasive approach to modulate the intestinal microbiota.					
31283798	10	52	theme	bone	1700:1703	arg1	density					1713:1719	the bone mineral density	1696:1719	the bone mineral density in mice	1696:1727	As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks.					
31283798	2	53	theme	mouse	301:305	arg1	models					307:312	mouse models	301:312	mouse models	301:312	Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome.					
31283798	1	54	theme	intestinal	174:183	arg1	microbiome					185:194	The intestinal microbiome	170:194	The intestinal microbiome	170:194	The intestinal microbiome is perturbed in patients with new-onset and chronic autoimmune inflammatory arthritis.					
31283798	4	55	theme	intestinal	737:746	arg1	microbiota					748:757	the intestinal microbiota	733:757	the intestinal microbiota	733:757	Prebiotics, defined as non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms, provide a relatively non-invasive approach to modulate the intestinal microbiota.					
31283798	10	56	theme	dietary	1734:1740	arg1	supplementation					1742:1756	dietary supplementation	1734:1756	dietary supplementation with scGOS/lcFOS for 8 weeks	1734:1785	As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks.					
31283798	0	57	theme	IL-1	129:132	arg1	antagonist					143:152	IL-1 receptor antagonist	129:152	IL-1 receptor antagonist deficient mice	129:167	Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.					
31283798	5	58	theme	%	924:924	arg1	fructo-oligosaccharides					937:959	10% long-chain fructo-oligosaccharides	922:959	10% long-chain fructo-oligosaccharides (scGOS/lcFOS)	922:973	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	5	58	theme	%	924:924	arg1	scGOS/lcFOS					962:972	scGOS/lcFOS	962:972	scGOS/lcFOS	962:972	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	8	59	theme	scGOS/lcFOS	1394:1404	arg1	diet					1406:1409	scGOS/lcFOS diet	1394:1409	scGOS/lcFOS diet	1394:1409	In contrast, the family Lachnospiraceae and the genus Lactobacillus, both associated with healthy microbiota, increased in mice receiving scGOS/lcFOS diet.					
31283798	0	60	theme	antagonist	143:152	arg1	mice					164:167	IL-1 receptor antagonist deficient mice	129:167	IL-1 receptor antagonist deficient mice	129:167	Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.					
31283798	5	61	theme	fructo-oligosaccharides	937:959	arg1	mixture					866:872	a prebiotic mixture	854:872	a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS)	854:973	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	11	62	theme	arthritis-prone	1973:1987	arg1	mice					1989:1992	arthritis-prone mice	1973:1992	arthritis-prone mice	1973:1992	Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.					
31283798	5	63	from	potential	812:820	arg1	arthritis					991:999	experimental arthritis	978:999	experimental arthritis in mice	978:1007	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	2	64	theme	autoimmune	358:367	arg1	arthritis					369:377	autoimmune arthritis	358:377	autoimmune arthritis	358:377	Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome.					
31283798	5	65	theme	supplementation	833:847	arg1	potential					812:820	the therapeutic potential	796:820	the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice	796:1007	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	6	66	theme	fecal	1119:1123	arg1	microbiota					1125:1134	the fecal microbiota	1115:1134	the fecal microbiota	1115:1134	We here show that dietary supplementation with scGOS/lcFOS has a pronounced effect on the composition of the fecal microbiota.					
31283798	11	67	from	able	1864:1867	arg1	mice					1989:1992	arthritis-prone mice	1973:1992	arthritis-prone mice	1973:1992	Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.					
31283798	11	68	theme	healthy	1891:1897	arg1	microbiota					1903:1912	presumably healthy gut microbiota	1880:1912	presumably healthy gut microbiota	1880:1912	Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.					
31283798	4	69	theme	non-digestible	568:581	arg1	carbohydrates					583:595	non-digestible carbohydrates	568:595	non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms	568:675	Prebiotics, defined as non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms, provide a relatively non-invasive approach to modulate the intestinal microbiota.					
31283798	11	70	theme	mineral	1931:1937	arg1	density					1939:1945	bone mineral density	1926:1945	bone mineral density	1926:1945	Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.					
31283798	0	71	theme	arthritis	103:111	arg1	development					113:123	arthritis development	103:123	arthritis development	103:123	Supplementation of diet with non-digestible oligosaccharides alters the intestinal microbiota, but not arthritis development, in IL-1 receptor antagonist deficient mice.					
31283798	9	72	theme	induced	1437:1443	arg1	sufficient					1587:1596	sufficient	1587:1596	sufficient	1587:1596	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	9	72	theme	induced	1437:1443	arg1	alterations					1445:1455	the scGOS/lcFOS induced alterations	1421:1455	the scGOS/lcFOS induced alterations of the intestinal microbiota	1421:1484	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	11	73	from	mice	1989:1992	arg1	able					1864:1867	able	1864:1867	able	1864:1867	Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.					
31283798	3	74	theme	interesting	482:492	arg1	approach					500:507	an interesting novel approach	479:507	an interesting novel approach to suppress inflammatory arthritis	479:542	This makes modulation of the intestinal microbiota an interesting novel approach to suppress inflammatory arthritis.					
31283798	5	75	theme	short-chain	881:891	arg1	galacto-oligosaccharides					893:916	90% short-chain galacto-oligosaccharides	877:916	90% short-chain galacto-oligosaccharides	877:916	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	9	76	theme	intestinal	1464:1473	arg1	microbiota					1475:1484	the intestinal microbiota	1460:1484	the intestinal microbiota	1460:1484	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
31283798	3	77	theme	microbiota	468:477	arg1	modulation					439:448	modulation	439:448	modulation of the intestinal microbiota	439:477	This makes modulation of the intestinal microbiota an interesting novel approach to suppress inflammatory arthritis.					
31283798	11	78	theme	scGOS/lcFOS	1833:1843	arg1	supplementation					1845:1859	dietary scGOS/lcFOS supplementation	1825:1859	dietary scGOS/lcFOS supplementation	1825:1859	Altogether, this study suggests that dietary scGOS/lcFOS supplementation is able to promote presumably healthy gut microbiota and improve bone mineral density, but not inflammation, in arthritis-prone mice.					
31283798	1	79	theme	autoimmune	248:257	arg1	arthritis					272:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	The intestinal microbiome is perturbed in patients with new-onset and chronic autoimmune inflammatory arthritis.					
31283798	5	80	theme	90	877:878	arg1	%					879:879	%	879:879	%	879:879	The aim of this study was to assess the therapeutic potential of dietary supplementation with a prebiotic mixture of 90% short-chain galacto-oligosaccharides and 10% long-chain fructo-oligosaccharides (scGOS/lcFOS) in experimental arthritis in mice.					
31283798	10	81	from	increase	1684:1691	arg1	density					1713:1719	the bone mineral density	1696:1719	the bone mineral density in mice	1696:1727	As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks.					
31283798	2	82	theme	arthritis	369:377	arg1	development					327:337	development	327:337	development	327:337	Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome.					
31283798	2	82	theme	arthritis	369:377	arg1	progression					343:353	progression	343:353	progression	343:353	Recent studies in mouse models suggest that development and progression of autoimmune arthritis is highly affected by the intestinal microbiome.					
31283798	1	83	theme	chronic	240:246	arg1	arthritis					272:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	The intestinal microbiome is perturbed in patients with new-onset and chronic autoimmune inflammatory arthritis.					
31283798	10	84	with	supplementation	1742:1756	arg1	scGOS/lcFOS					1763:1773	scGOS/lcFOS	1763:1773	scGOS/lcFOS	1763:1773	As expected, we did observe a significant increase in the bone mineral density in mice upon dietary supplementation with scGOS/lcFOS for 8 weeks.					
31283798	4	85	theme	non-invasive	699:710	arg1	approach					712:719	a relatively non-invasive approach	686:719	a relatively non-invasive approach to modulate the intestinal microbiota	686:757	Prebiotics, defined as non-digestible carbohydrates that selectively stimulate the growth and activity of beneficial microorganisms, provide a relatively non-invasive approach to modulate the intestinal microbiota.					
31283798	1	86	theme	inflammatory	259:270	arg1	arthritis					272:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	new-onset and chronic autoimmune inflammatory arthritis	226:280	The intestinal microbiome is perturbed in patients with new-onset and chronic autoimmune inflammatory arthritis.					
31283798	9	87	theme	T	1552:1552	arg1	subsets					1566:1572	the intestinal and systemic T helper cell subsets	1524:1572	the intestinal and systemic T helper cell subsets	1524:1572	However, the scGOS/lcFOS induced alterations of the intestinal microbiota did not induce significant effects on the intestinal and systemic T helper cell subsets and were not sufficient to reproducibly suppress arthritis in mice.					
30409998	12	0	theme	inflammation	1576:1587	arg1	manifestations					1550:1563	manifestations	1550:1563	manifestations of hepatic inflammation	1550:1587	Overall, inulin did not reduce hypercholesterolemia or atherosclerosis development in E3L.CETP mice despite showing clear prebiotic activity, but resulted in manifestations of hepatic inflammation when combined with a high percentage of dietary cholesterol.					
30409998	12	1	theme	atherosclerosis	1447:1461	arg1	development					1463:1473	atherosclerosis development	1447:1473	atherosclerosis development	1447:1473	Overall, inulin did not reduce hypercholesterolemia or atherosclerosis development in E3L.CETP mice despite showing clear prebiotic activity, but resulted in manifestations of hepatic inflammation when combined with a high percentage of dietary cholesterol.					
30409998	10	2	theme	mild	1177:1180	arg1	steatosis					1182:1190	mild steatosis	1177:1190	mild steatosis	1177:1190	Analogously, inulin induced mild steatosis and increased hepatocyte size, but did not affect hepatic triglyceride content.					
30409998	6	3	theme	lipid	674:678	arg1	levels					680:685	plasma lipid levels	667:685	plasma lipid levels	667:685	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	7	4	theme	SCFAs	864:868	arg1	genera					828:833	specific bacterial genera	809:833	specific bacterial genera	809:833	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	7	4	theme	SCFAs	864:868	arg1	levels					848:853	elevated levels	839:853	elevated levels of cecal SCFAs	839:868	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	11	5	theme	inflammation	1370:1381	arg1	markers					1383:1389	inflammation markers	1370:1389	inflammation markers	1370:1389	Inulin with 0.1% dietary cholesterol did not affect hepatic morphology, nor hepatic expression of inflammation markers.					
30409998	12	6	theme	E3L.CETP	1478:1485	arg1	mice					1487:1490	E3L.CETP mice	1478:1490	E3L.CETP mice	1478:1490	Overall, inulin did not reduce hypercholesterolemia or atherosclerosis development in E3L.CETP mice despite showing clear prebiotic activity, but resulted in manifestations of hepatic inflammation when combined with a high percentage of dietary cholesterol.					
30409998	8	7	theme	markers	1056:1062	arg1	expression					1029:1038	increased expression	1019:1038	increased expression of inflammation markers	1019:1062	Surprisingly, inulin resulted in mild hepatic inflammation as shown by increased expression of inflammation markers.					
30409998	7	8	with	Inulin	762:767	arg1	cholesterol					787:797	0.5% dietary cholesterol	774:797	0.5% dietary cholesterol	774:797	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	8	9	theme	mild	981:984	arg1	inflammation					994:1005	mild hepatic inflammation	981:1005	mild hepatic inflammation	981:1005	Surprisingly, inulin resulted in mild hepatic inflammation as shown by increased expression of inflammation markers.					
30409998	10	10	theme	hepatocyte	1206:1215	arg1	size					1217:1220	increased hepatocyte size	1196:1220	increased hepatocyte size	1196:1220	Analogously, inulin induced mild steatosis and increased hepatocyte size, but did not affect hepatic triglyceride content.					
30409998	0	11	theme	APOE	110:113	arg1	*					114:114	hypercholesterolemic APOE*3-Leiden	89:122	hypercholesterolemic APOE*3-Leiden	89:122	The prebiotic inulin modulates gut microbiota but does not ameliorate atherosclerosis in hypercholesterolemic APOE*3-Leiden.					
30409998	8	12	theme	increased	1019:1027	arg1	expression					1029:1038	increased expression	1019:1038	increased expression of inflammation markers	1019:1062	Surprisingly, inulin resulted in mild hepatic inflammation as shown by increased expression of inflammation markers.					
30409998	11	13	theme	%	1287:1287	arg1	cholesterol					1297:1307	0.1% dietary cholesterol	1284:1307	0.1% dietary cholesterol	1284:1307	Inulin with 0.1% dietary cholesterol did not affect hepatic morphology, nor hepatic expression of inflammation markers.					
30409998	10	14	theme	increased	1196:1204	arg1	size					1217:1220	increased hepatocyte size	1196:1220	increased hepatocyte size	1196:1220	Analogously, inulin induced mild steatosis and increased hepatocyte size, but did not affect hepatic triglyceride content.					
30409998	0	15	theme	hypercholesterolemic	89:108	arg1	*					114:114	hypercholesterolemic APOE*3-Leiden	89:122	hypercholesterolemic APOE*3-Leiden	89:122	The prebiotic inulin modulates gut microbiota but does not ameliorate atherosclerosis in hypercholesterolemic APOE*3-Leiden.					
30409998	9	16	theme	increased	1112:1120	arg1	number					1141:1146	increased hepatic macrophage number	1112:1146	increased hepatic macrophage number	1112:1146	However, these effects were not accompanied by increased hepatic macrophage number.					
30409998	4	17	theme	CETP	421:424	arg1	mice					437:440	CETP (E3L.CETP) mice	421:440	CETP (E3L.CETP) mice	421:440	CETP (E3L.CETP) mice.					
30409998	7	18	theme	dietary	779:785	arg1	cholesterol					787:797	0.5% dietary cholesterol	774:797	0.5% dietary cholesterol	774:797	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	6	19	theme	plasma	667:672	arg1	levels					680:685	plasma lipid levels	667:685	plasma lipid levels	667:685	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	3	20	from	development	392:402	arg1	*					411:411	APOE*3-Leiden	407:419	APOE*3-Leiden	407:419	Since the prebiotic inulin is thought to beneficially affect gut microbiota, we aimed to determine the effect of inulin supplementation on atherosclerosis development in APOE*3-Leiden.					
30409998	7	21	theme	atherosclerosis	919:933	arg1	development					935:945	atherosclerosis development	919:945	atherosclerosis development	919:945	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	5	22	contain	containing	493:502	arg2	cholesterol					517:527	0.1% or 0.5% cholesterol	504:527	0.1% or 0.5% cholesterol	504:527	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	5	22	contain	containing	493:502	arg1	diet					488:491	a western-type diet	473:491	a western-type diet containing 0.1% or 0.5% cholesterol	473:527	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	7	23	theme	elevated	839:846	arg1	levels					848:853	elevated levels	839:853	elevated levels of cecal SCFAs	839:868	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	3	24	theme	inulin	350:355	arg1	supplementation					357:371	inulin supplementation	350:371	inulin supplementation	350:371	Since the prebiotic inulin is thought to beneficially affect gut microbiota, we aimed to determine the effect of inulin supplementation on atherosclerosis development in APOE*3-Leiden.					
30409998	5	25	theme	%	547:547	arg1	inulin					549:554	10% inulin	545:554	10% inulin	545:554	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	6	26	theme	inulin	572:577	arg1	effects					561:567	The effects	557:567	The effects of inulin	557:577	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	5	27	theme	0.1	504:506	arg1	%					507:507	%	507:507	%	507:507	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	0	28	theme	prebiotic	4:12	arg1	inulin					14:19	The prebiotic inulin	0:19	The prebiotic inulin	0:19	The prebiotic inulin modulates gut microbiota but does not ameliorate atherosclerosis in hypercholesterolemic APOE*3-Leiden.					
30409998	2	29	theme	Gut	135:137	arg1	microbiota					139:148	Gut microbiota	135:148	Gut microbiota	135:148	Gut microbiota have been implicated in the development of atherosclerosis and cardiovascular disease.					
30409998	3	30	theme	prebiotic	247:255	arg1	inulin					257:262	the prebiotic inulin	243:262	the prebiotic inulin	243:262	Since the prebiotic inulin is thought to beneficially affect gut microbiota, we aimed to determine the effect of inulin supplementation on atherosclerosis development in APOE*3-Leiden.					
30409998	11	31	theme	dietary	1289:1295	arg1	cholesterol					1297:1307	0.1% dietary cholesterol	1284:1307	0.1% dietary cholesterol	1284:1307	Inulin with 0.1% dietary cholesterol did not affect hepatic morphology, nor hepatic expression of inflammation markers.					
30409998	12	32	theme	prebiotic	1514:1522	arg1	activity					1524:1531	clear prebiotic activity	1508:1531	clear prebiotic activity	1508:1531	Overall, inulin did not reduce hypercholesterolemia or atherosclerosis development in E3L.CETP mice despite showing clear prebiotic activity, but resulted in manifestations of hepatic inflammation when combined with a high percentage of dietary cholesterol.					
30409998	5	33	theme	%	507:507	arg1	cholesterol					517:527	0.1% or 0.5% cholesterol	504:527	0.1% or 0.5% cholesterol	504:527	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	3	34	theme	APOE	407:410	arg1	*					411:411	APOE*3-Leiden	407:419	APOE*3-Leiden	407:419	Since the prebiotic inulin is thought to beneficially affect gut microbiota, we aimed to determine the effect of inulin supplementation on atherosclerosis development in APOE*3-Leiden.					
30409998	7	35	theme	0.5	774:776	arg1	%					777:777	%	777:777	%	777:777	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	8	36	theme	inflammation	1043:1054	arg1	markers					1056:1062	inflammation markers	1043:1062	inflammation markers	1043:1062	Surprisingly, inulin resulted in mild hepatic inflammation as shown by increased expression of inflammation markers.					
30409998	6	37	theme	hepatic	740:746	arg1	inflammation					748:759	hepatic inflammation	740:759	hepatic inflammation	740:759	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	11	38	theme	0.1	1284:1286	arg1	%					1287:1287	%	1287:1287	%	1287:1287	Inulin with 0.1% dietary cholesterol did not affect hepatic morphology, nor hepatic expression of inflammation markers.					
30409998	3	39	theme	atherosclerosis	376:390	arg1	development					392:402	atherosclerosis development	376:402	atherosclerosis development in APOE*3-Leiden	376:419	Since the prebiotic inulin is thought to beneficially affect gut microbiota, we aimed to determine the effect of inulin supplementation on atherosclerosis development in APOE*3-Leiden.					
30409998	7	40	theme	%	777:777	arg1	cholesterol					787:797	0.5% dietary cholesterol	774:797	0.5% dietary cholesterol	774:797	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	7	41	theme	cecal	858:862	arg1	SCFAs					864:868	cecal SCFAs	858:868	cecal SCFAs	858:868	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	7	42	theme	plasma	890:895	arg1	levels					909:914	plasma cholesterol levels	890:914	plasma cholesterol levels	890:914	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	7	43	theme	specific	809:816	arg1	genera					828:833	specific bacterial genera	809:833	specific bacterial genera	809:833	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	3	44	theme	gut	298:300	arg1	microbiota					302:311	gut microbiota	298:311	gut microbiota	298:311	Since the prebiotic inulin is thought to beneficially affect gut microbiota, we aimed to determine the effect of inulin supplementation on atherosclerosis development in APOE*3-Leiden.					
30409998	6	45	theme	fatty	641:645	arg1	SCFA					653:656	SCFA	653:656	SCFA	653:656	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	6	45	theme	fatty	641:645	arg1	acid					647:650	short-chain fatty acid	629:650	cecal short-chain fatty acid (SCFA) levels	623:664	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	6	46	theme	acid	647:650	arg1	levels					659:664	cecal short-chain fatty acid (SCFA) levels	623:664	cecal short-chain fatty acid (SCFA) levels	623:664	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	11	47	theme	hepatic	1348:1354	arg1	expression					1356:1365	hepatic expression	1348:1365	hepatic expression	1348:1365	Inulin with 0.1% dietary cholesterol did not affect hepatic morphology, nor hepatic expression of inflammation markers.					
30409998	12	48	theme	cholesterol	1637:1647	arg1	percentage					1615:1624	a high percentage	1608:1624	a high percentage of dietary cholesterol	1608:1647	Overall, inulin did not reduce hypercholesterolemia or atherosclerosis development in E3L.CETP mice despite showing clear prebiotic activity, but resulted in manifestations of hepatic inflammation when combined with a high percentage of dietary cholesterol.					
30409998	0	49	from	atherosclerosis	70:84	arg1	*					114:114	hypercholesterolemic APOE*3-Leiden	89:122	hypercholesterolemic APOE*3-Leiden	89:122	The prebiotic inulin modulates gut microbiota but does not ameliorate atherosclerosis in hypercholesterolemic APOE*3-Leiden.					
30409998	5	50	theme	western-type	475:486	arg1	diet					488:491	a western-type diet	473:491	a western-type diet containing 0.1% or 0.5% cholesterol	473:527	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	0	51	theme	gut	31:33	arg1	microbiota					35:44	gut microbiota	31:44	gut microbiota	31:44	The prebiotic inulin modulates gut microbiota but does not ameliorate atherosclerosis in hypercholesterolemic APOE*3-Leiden.					
30409998	10	52	theme	triglyceride	1250:1261	arg1	content					1263:1269	hepatic triglyceride content	1242:1269	hepatic triglyceride content	1242:1269	Analogously, inulin induced mild steatosis and increased hepatocyte size, but did not affect hepatic triglyceride content.					
30409998	12	53	theme	high	1610:1613	arg1	percentage					1615:1624	a high percentage	1608:1624	a high percentage of dietary cholesterol	1608:1647	Overall, inulin did not reduce hypercholesterolemia or atherosclerosis development in E3L.CETP mice despite showing clear prebiotic activity, but resulted in manifestations of hepatic inflammation when combined with a high percentage of dietary cholesterol.					
30409998	3	54	theme	supplementation	357:371	arg1	effect					340:345	the effect	336:345	the effect of inulin supplementation on atherosclerosis development in APOE*3-Leiden	336:419	Since the prebiotic inulin is thought to beneficially affect gut microbiota, we aimed to determine the effect of inulin supplementation on atherosclerosis development in APOE*3-Leiden.					
30409998	12	55	theme	hepatic	1568:1574	arg1	inflammation					1576:1587	hepatic inflammation	1568:1587	hepatic inflammation	1568:1587	Overall, inulin did not reduce hypercholesterolemia or atherosclerosis development in E3L.CETP mice despite showing clear prebiotic activity, but resulted in manifestations of hepatic inflammation when combined with a high percentage of dietary cholesterol.					
30409998	6	56	theme	hepatic	717:723	arg1	morphology					725:734	hepatic morphology	717:734	hepatic morphology	717:734	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	2	57	theme	cardiovascular	213:226	arg1	disease					228:234	cardiovascular disease	213:234	cardiovascular disease	213:234	Gut microbiota have been implicated in the development of atherosclerosis and cardiovascular disease.					
30409998	2	58	theme	disease	228:234	arg1	development					178:188	the development	174:188	the development of atherosclerosis and cardiovascular disease	174:234	Gut microbiota have been implicated in the development of atherosclerosis and cardiovascular disease.					
30409998	9	59	theme	hepatic	1122:1128	arg1	number					1141:1146	increased hepatic macrophage number	1112:1146	increased hepatic macrophage number	1112:1146	However, these effects were not accompanied by increased hepatic macrophage number.					
30409998	1	60	theme	CETP	124:127	arg1	mice					129:132	CETP mice	124:132	CETP mice.	124:133	CETP mice.					
30409998	6	61	theme	short-chain	629:639	arg1	SCFA					653:656	SCFA	653:656	SCFA	653:656	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	6	61	theme	short-chain	629:639	arg1	acid					647:650	short-chain fatty acid	629:650	cecal short-chain fatty acid (SCFA) levels	623:664	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	8	62	theme	hepatic	986:992	arg1	inflammation					994:1005	mild hepatic inflammation	981:1005	mild hepatic inflammation	981:1005	Surprisingly, inulin resulted in mild hepatic inflammation as shown by increased expression of inflammation markers.					
30409998	7	63	theme	cholesterol	897:907	arg1	levels					909:914	plasma cholesterol levels	890:914	plasma cholesterol levels	890:914	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	5	64	theme	10	545:546	arg1	%					547:547	%	547:547	%	547:547	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	5	65	theme	Female	443:448	arg1	mice					459:462	Female E3L.CETP mice	443:462	Female E3L.CETP mice	443:462	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	10	66	theme	hepatic	1242:1248	arg1	content					1263:1269	hepatic triglyceride content	1242:1269	hepatic triglyceride content	1242:1269	Analogously, inulin induced mild steatosis and increased hepatocyte size, but did not affect hepatic triglyceride content.					
30409998	9	67	theme	macrophage	1130:1139	arg1	number					1141:1146	increased hepatic macrophage number	1112:1146	increased hepatic macrophage number	1112:1146	However, these effects were not accompanied by increased hepatic macrophage number.					
30409998	2	68	theme	atherosclerosis	193:207	arg1	development					178:188	the development	174:188	the development of atherosclerosis and cardiovascular disease	174:234	Gut microbiota have been implicated in the development of atherosclerosis and cardiovascular disease.					
30409998	12	69	theme	clear	1508:1512	arg1	activity					1524:1531	clear prebiotic activity	1508:1531	clear prebiotic activity	1508:1531	Overall, inulin did not reduce hypercholesterolemia or atherosclerosis development in E3L.CETP mice despite showing clear prebiotic activity, but resulted in manifestations of hepatic inflammation when combined with a high percentage of dietary cholesterol.					
30409998	6	70	theme	atherosclerosis	688:702	arg1	development					704:714	atherosclerosis development	688:714	atherosclerosis development	688:714	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	11	71	with	Inulin	1272:1277	arg1	cholesterol					1297:1307	0.1% dietary cholesterol	1284:1307	0.1% dietary cholesterol	1284:1307	Inulin with 0.1% dietary cholesterol did not affect hepatic morphology, nor hepatic expression of inflammation markers.					
30409998	5	72	theme	E3L.CETP	450:457	arg1	mice					459:462	Female E3L.CETP mice	443:462	Female E3L.CETP mice	443:462	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	7	73	theme	bacterial	818:826	arg1	genera					828:833	specific bacterial genera	809:833	specific bacterial genera	809:833	Inulin with 0.5% dietary cholesterol increased specific bacterial genera and elevated levels of cecal SCFAs, but did not affect plasma cholesterol levels or atherosclerosis development.					
30409998	11	74	theme	markers	1383:1389	arg1	morphology					1332:1341	hepatic morphology	1324:1341	hepatic morphology	1324:1341	Inulin with 0.1% dietary cholesterol did not affect hepatic morphology, nor hepatic expression of inflammation markers.					
30409998	3	75	from	effect	340:345	arg1	development					392:402	atherosclerosis development	376:402	atherosclerosis development in APOE*3-Leiden	376:419	Since the prebiotic inulin is thought to beneficially affect gut microbiota, we aimed to determine the effect of inulin supplementation on atherosclerosis development in APOE*3-Leiden.					
30409998	12	76	theme	dietary	1629:1635	arg1	cholesterol					1637:1647	dietary cholesterol	1629:1647	dietary cholesterol	1629:1647	Overall, inulin did not reduce hypercholesterolemia or atherosclerosis development in E3L.CETP mice despite showing clear prebiotic activity, but resulted in manifestations of hepatic inflammation when combined with a high percentage of dietary cholesterol.					
30409998	5	77	theme	0.5	512:514	arg1	%					515:515	%	515:515	%	515:515	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	6	78	theme	cecal	623:627	arg1	levels					659:664	cecal short-chain fatty acid (SCFA) levels	623:664	cecal short-chain fatty acid (SCFA) levels	623:664	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
30409998	5	79	theme	%	515:515	arg1	cholesterol					517:527	0.1% or 0.5% cholesterol	504:527	0.1% or 0.5% cholesterol	504:527	Female E3L.CETP mice were fed a western-type diet containing 0.1% or 0.5% cholesterol with or without 10% inulin.					
30409998	11	80	theme	hepatic	1324:1330	arg1	morphology					1332:1341	hepatic morphology	1324:1341	hepatic morphology	1324:1341	Inulin with 0.1% dietary cholesterol did not affect hepatic morphology, nor hepatic expression of inflammation markers.					
30409998	6	81	theme	microbiota	599:608	arg1	composition					610:620	microbiota composition	599:620	microbiota composition	599:620	The effects of inulin were determined on: microbiota composition, cecal short-chain fatty acid (SCFA) levels, plasma lipid levels, atherosclerosis development, hepatic morphology and hepatic inflammation.					
29497108	6	0	theme	cells	1197:1201	arg1	development					1160:1170	development	1160:1170	development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo	1160:1305	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	4	1	theme	insulitis	781:789	arg1	development					748:758	development	748:758	development of severe pancreatic insulitis in later life	748:803	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	6	2	theme	tolerogenic	1175:1185	arg1	tDCs					1204:1207	tDCs	1204:1207	tDCs	1204:1207	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	6	2	theme	tolerogenic	1175:1185	arg1	priming					1211:1217	priming	1211:1217	priming of functional regulatory T cells, which support the protective effects detected in vivo	1211:1305	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	6	2	theme	tolerogenic	1175:1185	arg1	cells					1197:1201	tolerogenic dendritic cells	1175:1201	tolerogenic dendritic cells (tDCs)	1175:1208	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	7	3	from	milk	1345:1348	arg1	present					1328:1334	present	1328:1334	present	1328:1334	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	4	4	theme	later	794:798	arg1	life					800:803	later life	794:803	later life	794:803	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	5	5	theme	cytokine	1089:1096	arg1	profiles					1098:1105	anti-diabetogenic cytokine profiles	1071:1105	anti-diabetogenic cytokine profiles	1071:1105	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	6	theme	protective	812:821	arg1	effects					823:829	These protective effects	806:829	These protective effects	806:829	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	4	7	theme	1	584:584	arg1	%					585:585	%	585:585	%	585:585	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	6	8	theme	cells	1246:1250	arg1	priming					1211:1217	priming	1211:1217	priming of functional regulatory T cells, which support the protective effects detected in vivo	1211:1305	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	6	8	theme	cells	1246:1250	arg1	cells					1197:1201	tolerogenic dendritic cells	1175:1201	tolerogenic dendritic cells (tDCs)	1175:1208	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	4	9	dep	period	560:565	arg1	only					549:552	only	549:552	only	549:552	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	1	10	theme	optimal	179:185	arg1	development					198:208	optimal microbiome development	179:208	optimal microbiome development during early life	179:226	Development of Type 1 diabetes (T1D) is influenced by non-genetic factors, such as optimal microbiome development during early life that "programs" the immune system.					
29497108	4	11	theme	authentic	587:595	arg1	HMOS					597:600	1% authentic HMOS	584:600	1% authentic HMOS	584:600	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	6	12	theme	regulatory	1233:1242	arg1	cells					1246:1250	functional regulatory T cells	1222:1250	functional regulatory T cells	1222:1250	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	7	13	from	vital	1378:1382	arg1	protection					1391:1400	the protection	1387:1400	the protection of children at risk for T1D	1387:1428	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	6	14	dep	in	1118:1119	arg1	vitro					1121:1125	vitro	1121:1125	vitro	1121:1125	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	7	15	attach	present	1328:1334	arg1	milk					1345:1348	human milk	1339:1348	human milk	1339:1348	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	7	15	attach	present	1328:1334	arg2	HMOS					1323:1326	HMOS	1323:1326	HMOS present in human milk	1323:1348	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	4	16	from	development	748:758	arg1	life					800:803	later life	794:803	later life	794:803	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	1	17	theme	diabetes	118:125	arg1	Development					96:106	Development	96:106	Development of Type 1 diabetes (T1D)	96:131	Development of Type 1 diabetes (T1D) is influenced by non-genetic factors, such as optimal microbiome development during early life that "programs" the immune system.					
29497108	6	18	theme	in	1118:1119	arg1	HMOS					1127:1130	in vitro HMOS	1118:1130	in vitro HMOS combined with SCFAs	1118:1150	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	0	19	theme	autoimmune	63:72	arg1	diabetes					74:81	autoimmune diabetes	63:81	autoimmune diabetes in NOD-mice	63:93	Human milk oligosaccharides protect against the development of autoimmune diabetes in NOD-mice.					
29497108	7	20	theme	microbiota	1457:1466	arg1	development					1468:1478	immune and gut microbiota development	1442:1478	development	1468:1478	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	5	21	from	induction	1058:1066	arg1	composition					898:908	fecal microbiota composition	881:908	fecal microbiota composition	881:908	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	21	from	induction	1058:1066	arg1	fecal					1016:1020	fecal	1016:1020	fecal	1016:1020	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	21	from	induction	1058:1066	arg1	content					1040:1046	cecum content	1034:1046	cecum content	1034:1046	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	4	22	theme	T1D	693:695	arg1	development					697:707	T1D development	693:707	T1D development	693:707	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	3	23	dep	known	464:468	arg1	modulators					452:461	microbiota modulators	441:461	microbiota modulators	441:461	Human Milk Oligosaccharides (HMOS) are microbiota modulators, known to regulate immune responses directly.					
29497108	0	24	from	development	48:58	arg1	NOD-mice					86:93	NOD-mice	86:93	NOD-mice	86:93	Human milk oligosaccharides protect against the development of autoimmune diabetes in NOD-mice.					
29497108	5	25	theme	anti-inflammatory	915:931	arg1	changes					1005:1011	anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes	915:1011	ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content	911:1046	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	26	dep	induction	1058:1066	arg1	iii					1053:1055	iii	1053:1055	iii	1053:1055	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	3	27	theme	Milk	408:411	arg1	HMOS					431:434	HMOS	431:434	HMOS	431:434	Human Milk Oligosaccharides (HMOS) are microbiota modulators, known to regulate immune responses directly.					
29497108	3	27	theme	Milk	408:411	arg1	Oligosaccharides					413:428	Human Milk Oligosaccharides	402:428	Human Milk Oligosaccharides (HMOS)	402:435	Human Milk Oligosaccharides (HMOS) are microbiota modulators, known to regulate immune responses directly.					
29497108	0	28	theme	Human	0:4	arg1	oligosaccharides					11:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides protect against the development of autoimmune diabetes in NOD-mice.					
29497108	1	29	theme	immune	248:253	arg1	system					255:260	the immune system	244:260	the immune system	244:260	Development of Type 1 diabetes (T1D) is influenced by non-genetic factors, such as optimal microbiome development during early life that "programs" the immune system.					
29497108	5	30	theme	fecal	881:885	arg1	composition					898:908	fecal microbiota composition	881:908	fecal microbiota composition	881:908	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	4	31	theme	early	527:531	arg1	provision					538:546	early life provision	527:546	early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures),	527:668	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	3	32	theme	microbiota	441:450	arg1	modulators					452:461	microbiota modulators	441:461	microbiota modulators	441:461	Human Milk Oligosaccharides (HMOS) are microbiota modulators, known to regulate immune responses directly.					
29497108	1	33	theme	Type	111:114	arg1	T1D					128:130	T1D	128:130	T1D	128:130	Development of Type 1 diabetes (T1D) is influenced by non-genetic factors, such as optimal microbiome development during early life that "programs" the immune system.					
29497108	1	33	theme	Type	111:114	arg1	diabetes					118:125	Type 1 diabetes (T1D)	111:131	Type 1 diabetes (T1D)	111:131	Development of Type 1 diabetes (T1D) is influenced by non-genetic factors, such as optimal microbiome development during early life that "programs" the immune system.					
29497108	5	34	theme	chain	978:982	arg1	SCFAs					997:1001	SCFAs	997:1001	SCFAs	997:1001	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	34	theme	chain	978:982	arg1	acids					990:994	short chain fatty acids	972:994	short chain fatty acids (SCFAs)	972:1002	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	4	35	theme	severe	763:768	arg1	insulitis					781:789	severe pancreatic insulitis	763:789	severe pancreatic insulitis	763:789	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	4	36	theme	diabetic	722:729	arg1	mice					731:734	non-obese diabetic mice	712:734	non-obese diabetic mice	712:734	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	2	37	theme	independent	307:317	arg1	factor					330:335	an independent protective factor	304:335	an independent protective factor	304:335	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	2	37	theme	independent	307:317	arg1	breastfeeding					287:299	Exclusive and prolonged breastfeeding	263:299	Exclusive and prolonged breastfeeding	263:299	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	5	38	theme	metabolite	955:964	arg1	changes					1005:1011	anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes	915:1011	ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content	911:1046	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	4	39	theme	HMOS	597:600	arg1	provision					538:546	early life provision	527:546	early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures),	527:668	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	7	40	from	protection	1391:1400	arg1	risk					1417:1420	risk	1417:1420	risk for T1D	1417:1428	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	7	40	from	protection	1391:1400	arg1	vital					1378:1382	vital	1378:1382	vital	1378:1382	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	4	41	theme	weeks	574:578	arg1	period					560:565	a period	558:565	a period of six weeks	558:578	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	3	42	theme	immune	482:487	arg1	responses					489:497	immune responses	482:497	immune responses	482:497	Human Milk Oligosaccharides (HMOS) are microbiota modulators, known to regulate immune responses directly.					
29497108	2	43	theme	Exclusive	263:271	arg1	factor					330:335	an independent protective factor	304:335	an independent protective factor	304:335	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	2	43	theme	Exclusive	263:271	arg1	breastfeeding					287:299	Exclusive and prolonged breastfeeding	263:299	Exclusive and prolonged breastfeeding	263:299	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	7	44	from	risk	1417:1420	arg1	children					1405:1412	children	1405:1412	children at risk for T1D	1405:1428	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	7	44	from	risk	1417:1420	arg1	protection					1391:1400	the protection	1387:1400	the protection of children at risk for T1D	1387:1428	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	7	45	theme	human	1339:1343	arg1	milk					1345:1348	human milk	1339:1348	human milk	1339:1348	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	6	46	theme	dendritic	1187:1195	arg1	tDCs					1204:1207	tDCs	1204:1207	tDCs	1204:1207	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	6	46	theme	dendritic	1187:1195	arg1	priming					1211:1217	priming	1211:1217	priming of functional regulatory T cells, which support the protective effects detected in vivo	1211:1305	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	6	46	theme	dendritic	1187:1195	arg1	cells					1197:1201	tolerogenic dendritic cells	1175:1201	tolerogenic dendritic cells (tDCs)	1175:1208	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	1	47	theme	non-genetic	150:160	arg1	factors					162:168	non-genetic factors	150:168	non-genetic factors	150:168	Development of Type 1 diabetes (T1D) is influenced by non-genetic factors, such as optimal microbiome development during early life that "programs" the immune system.					
29497108	1	47	theme	non-genetic	150:160	arg1	development					198:208	optimal microbiome development	179:208	optimal microbiome development during early life	179:226	Development of Type 1 diabetes (T1D) is influenced by non-genetic factors, such as optimal microbiome development during early life that "programs" the immune system.					
29497108	4	48	theme	pancreatic	770:779	arg1	insulitis					781:789	severe pancreatic insulitis	763:789	severe pancreatic insulitis	763:789	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	7	49	theme	present	1328:1334	arg1	HMOS					1323:1326	HMOS	1323:1326	HMOS present in human milk	1323:1348	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	5	50	dep	acids	990:994	arg1	i.e.					967:970	i.e.	967:970	i.e.	967:970	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	2	51	theme	prolonged	277:285	arg1	factor					330:335	an independent protective factor	304:335	an independent protective factor	304:335	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	2	51	theme	prolonged	277:285	arg1	breastfeeding					287:299	Exclusive and prolonged breastfeeding	263:299	Exclusive and prolonged breastfeeding	263:299	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	0	52	from	NOD-mice	86:93	arg1	development					48:58	the development	44:58	the development of autoimmune diabetes in NOD-mice	44:93	Human milk oligosaccharides protect against the development of autoimmune diabetes in NOD-mice.					
29497108	6	53	theme	T	1244:1244	arg1	cells					1246:1250	functional regulatory T cells	1222:1250	functional regulatory T cells	1222:1250	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	1	54	theme	microbiome	187:196	arg1	development					198:208	optimal microbiome development	179:208	optimal microbiome development during early life	179:226	Development of Type 1 diabetes (T1D) is influenced by non-genetic factors, such as optimal microbiome development during early life that "programs" the immune system.					
29497108	0	55	from	diabetes	74:81	arg1	NOD-mice					86:93	NOD-mice	86:93	NOD-mice	86:93	Human milk oligosaccharides protect against the development of autoimmune diabetes in NOD-mice.					
29497108	5	56	dep	metabolite	955:964	arg1	SCFAs					997:1001	SCFAs	997:1001	SCFAs	997:1001	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	56	dep	metabolite	955:964	arg1	acids					990:994	short chain fatty acids	972:994	short chain fatty acids (SCFAs)	972:1002	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	6	57	theme	functional	1222:1231	arg1	cells					1246:1250	functional regulatory T cells	1222:1250	functional regulatory T cells	1222:1250	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	7	58	theme	early	1483:1487	arg1	life					1489:1492	early life	1483:1492	early life	1483:1492	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	5	59	theme	beneficial	855:864	arg1	alterations					866:876	beneficial alterations	855:876	i) beneficial alterations in fecal microbiota composition	852:908	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	4	60	theme	short-chain	645:655	arg1	structures					657:666	short-chain structures	645:666	short-chain structures	645:666	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	0	61	theme	diabetes	74:81	arg1	development					48:58	the development	44:58	the development of autoimmune diabetes in NOD-mice	44:93	Human milk oligosaccharides protect against the development of autoimmune diabetes in NOD-mice.					
29497108	7	62	theme	gut	1453:1455	arg1	development					1468:1478	immune and gut microbiota development	1442:1478	development	1468:1478	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	7	63	from	present	1328:1334	arg1	milk					1345:1348	human milk	1339:1348	human milk	1339:1348	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	7	64	theme	immune	1442:1447	arg1	development					1468:1478	immune and gut microbiota development	1442:1478	development	1468:1478	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	6	65	theme	protective	1271:1280	arg1	effects					1282:1288	the protective effects	1267:1288	the protective effects detected in vivo	1267:1305	Moreover, in vitro HMOS combined with SCFAs induced development of tolerogenic dendritic cells (tDCs), priming of functional regulatory T cells, which support the protective effects detected in vivo.					
29497108	5	66	dep	alterations	866:876	arg1	i					852:852	i	852:852	i	852:852	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	67	from	changes	1005:1011	arg1	composition					898:908	fecal microbiota composition	881:908	fecal microbiota composition	881:908	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	67	from	changes	1005:1011	arg1	fecal					1016:1020	fecal	1016:1020	fecal	1016:1020	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	67	from	changes	1005:1011	arg1	content					1040:1046	cecum content	1034:1046	cecum content	1034:1046	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	0	68	theme	milk	6:9	arg1	oligosaccharides					11:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides protect against the development of autoimmune diabetes in NOD-mice.					
29497108	5	69	theme	cecum	1034:1038	arg1	content					1040:1046	cecum content	1034:1046	cecum content	1034:1046	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	70	from	alterations	866:876	arg1	composition					898:908	fecal microbiota composition	881:908	fecal microbiota composition	881:908	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	70	from	alterations	866:876	arg1	fecal					1016:1020	fecal	1016:1020	fecal	1016:1020	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	70	from	alterations	866:876	arg1	content					1040:1046	cecum content	1034:1046	cecum content	1034:1046	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	4	71	theme	life	533:536	arg1	provision					538:546	early life provision	527:546	early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures),	527:668	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	2	72	theme	T1D	364:366	arg1	development					349:359	the development	345:359	the development of T1D	345:366	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	5	73	theme	profiles	1098:1105	arg1	induction					1058:1066	induction	1058:1066	iii) induction of anti-diabetogenic cytokine profiles	1053:1105	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	73	theme	profiles	1098:1105	arg1	changes					1005:1011	anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes	915:1011	ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content	911:1046	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	73	theme	profiles	1098:1105	arg1	alterations					866:876	beneficial alterations	855:876	i) beneficial alterations in fecal microbiota composition	852:908	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	74	theme	microbiota	887:896	arg1	composition					898:908	fecal microbiota composition	881:908	fecal microbiota composition	881:908	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	75	theme	short	972:976	arg1	SCFAs					997:1001	SCFAs	997:1001	SCFAs	997:1001	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	75	theme	short	972:976	arg1	acids					990:994	short chain fatty acids	972:994	short chain fatty acids (SCFAs)	972:1002	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	3	76	theme	Human	402:406	arg1	HMOS					431:434	HMOS	431:434	HMOS	431:434	Human Milk Oligosaccharides (HMOS) are microbiota modulators, known to regulate immune responses directly.					
29497108	3	76	theme	Human	402:406	arg1	Oligosaccharides					413:428	Human Milk Oligosaccharides	402:428	Human Milk Oligosaccharides (HMOS)	402:435	Human Milk Oligosaccharides (HMOS) are microbiota modulators, known to regulate immune responses directly.					
29497108	2	77	theme	bioactive	380:388	arg1	components					390:399	bioactive components	380:399	bioactive components	380:399	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	5	78	theme	fatty	984:988	arg1	SCFAs					997:1001	SCFAs	997:1001	SCFAs	997:1001	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	5	78	theme	fatty	984:988	arg1	acids					990:994	short chain fatty acids	972:994	short chain fatty acids (SCFAs)	972:1002	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	2	79	theme	protective	319:328	arg1	factor					330:335	an independent protective factor	304:335	an independent protective factor	304:335	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	2	79	theme	protective	319:328	arg1	breastfeeding					287:299	Exclusive and prolonged breastfeeding	263:299	Exclusive and prolonged breastfeeding	263:299	Exclusive and prolonged breastfeeding is an independent protective factor against the development of T1D, likely via bioactive components.					
29497108	5	80	theme	anti-diabetogenic	1071:1087	arg1	profiles					1098:1105	anti-diabetogenic cytokine profiles	1071:1105	anti-diabetogenic cytokine profiles	1071:1105	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	7	81	from	development	1468:1478	arg1	life					1489:1492	early life	1483:1492	early life	1483:1492	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	7	82	theme	children	1405:1412	arg1	protection					1391:1400	the protection	1387:1400	the protection of children at risk for T1D	1387:1428	In conclusion, HMOS present in human milk are therefore thought to be vital in the protection of children at risk for T1D, supporting immune and gut microbiota development in early life.					
29497108	5	83	theme	microbiota-generating	933:953	arg1	metabolite					955:964	microbiota-generating metabolite	933:964	ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content	911:1046	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
29497108	4	84	theme	non-obese	712:720	arg1	mice					731:734	non-obese diabetic mice	712:734	non-obese diabetic mice	712:734	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	1	85	theme	early	217:221	arg1	life					223:226	early life	217:226	early life	217:226	Development of Type 1 diabetes (T1D) is influenced by non-genetic factors, such as optimal microbiome development during early life that "programs" the immune system.					
29497108	4	86	theme	%	585:585	arg1	HMOS					597:600	1% authentic HMOS	584:600	1% authentic HMOS	584:600	Here we show that early life provision (only for a period of six weeks) of 1% authentic HMOS (consisting of both long-chain, as well as short-chain structures), delayed and suppressed T1D development in non-obese diabetic mice and reduced development of severe pancreatic insulitis in later life.					
29497108	5	87	dep	changes	1005:1011	arg1	ii					911:912	ii	911:912	ii	911:912	These protective effects were associated with i) beneficial alterations in fecal microbiota composition, ii) anti-inflammatory microbiota-generating metabolite (i.e. short chain fatty acids (SCFAs)) changes in fecal, as well as cecum content, and iii) induction of anti-diabetogenic cytokine profiles.					
31248580	2	0	theme	complex	347:353	arg1	glycoconjugates					367:381	complex and diverse glycoconjugates	347:381	complex and diverse glycoconjugates	347:381	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	6	1	theme	N-acetylneuraminic	891:908	arg1	acid					910:913	N-acetylneuraminic acid	891:913	N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	891:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	6	1	theme	N-acetylneuraminic	891:908	arg1	Neu5Ac					916:921	Neu5Ac	916:921	Neu5Ac	916:921	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	9	2	theme	%	1609:1609	arg1	coefficient					1562:1572	A coefficient	1560:1572	A coefficient of variation (CV) of 4,98% and 4,40%, respectively	1560:1623	A coefficient of variation (CV) of 4,98% and 4,40%, respectively was obtained and an optimal dispersion variability expressed by the SD and the CV was also reported, confirming the efficiency of the methodology.					
31248580	11	3	theme	residues	1924:1931	arg1	presence					1906:1913	The presence	1902:1913	The presence of these residues	1902:1931	The presence of these residues contributes to a strong anionic shield and may provide an hydrating and protective barrier as well as a repulsive structure that is crucial to lung physiology.					
31248580	11	4	theme	repulsive	2037:2045	arg1	crucial					2065:2071	crucial	2065:2071	crucial	2065:2071	The presence of these residues contributes to a strong anionic shield and may provide an hydrating and protective barrier as well as a repulsive structure that is crucial to lung physiology.					
31248580	11	4	theme	repulsive	2037:2045	arg1	structure					2047:2055	a repulsive structure	2035:2055	an hydrating and protective barrier as well as a repulsive structure that is crucial to lung physiology	1988:2090	The presence of these residues contributes to a strong anionic shield and may provide an hydrating and protective barrier as well as a repulsive structure that is crucial to lung physiology.					
31248580	7	5	theme	rat	1094:1096	arg1	homogenates					1103:1113	adult rat lung homogenates	1088:1113	adult rat lung homogenates	1088:1113	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	4	6	theme	physiological	656:668	arg1	functions					687:695	many physiological and pathological functions	651:695	many physiological and pathological functions	651:695	Due to their abundance and their terminal position in glycans, sialic acids are implicated in many physiological and pathological functions.					
31248580	10	7	theme	purified	1877:1884	arg1	membranes					1891:1899	purified lung membranes	1877:1899	purified lung membranes	1877:1899	To the best of our knowledge, our group was the first to identify, separate and quantify sialic acids in purified lung membranes.					
31248580	4	8	theme	sialic	620:625	arg1	acids					627:631	sialic acids	620:631	sialic acids	620:631	Due to their abundance and their terminal position in glycans, sialic acids are implicated in many physiological and pathological functions.					
31248580	1	9	theme	tree-like	202:210	arg1	lung					161:164	The lung	157:164	The lung	157:164	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	1	9	theme	tree-like	202:210	arg1	structure					212:220	a tree-like structure	200:220	a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells	200:327	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	6	10	theme	Neu5Gc	951:956	arg1	residues					959:966	N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues	891:966	N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	891:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	9	11	theme	dispersion	1653:1662	arg1	variability					1664:1674	an optimal dispersion variability	1642:1674	an optimal dispersion variability expressed by the SD and the CV	1642:1705	A coefficient of variation (CV) of 4,98% and 4,40%, respectively was obtained and an optimal dispersion variability expressed by the SD and the CV was also reported, confirming the efficiency of the methodology.					
31248580	0	12	from	quantification	76:89	arg1	lung					151:154	the lung	147:154	the lung	147:154	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	0	12	from	quantification	76:89	arg1	cells					47:51	bronchioloalveolar cells	28:51	bronchioloalveolar cells	28:51	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	6	13	theme	acid	945:948	arg1	residues					959:966	N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues	891:966	N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	891:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	11	14	theme	hydrating	1991:1999	arg1	barrier					2016:2022	an hydrating and protective barrier	1988:2022	an hydrating and protective barrier as well as a repulsive structure that is crucial to lung physiology	1988:2090	The presence of these residues contributes to a strong anionic shield and may provide an hydrating and protective barrier as well as a repulsive structure that is crucial to lung physiology.					
31248580	0	15	from	identification	57:70	arg1	lung					151:154	the lung	147:154	the lung	147:154	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	0	15	from	identification	57:70	arg1	cells					47:51	bronchioloalveolar cells	28:51	bronchioloalveolar cells	28:51	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	11	16	theme	protective	2005:2014	arg1	barrier					2016:2022	an hydrating and protective barrier	1988:2022	an hydrating and protective barrier as well as a repulsive structure that is crucial to lung physiology	1988:2090	The presence of these residues contributes to a strong anionic shield and may provide an hydrating and protective barrier as well as a repulsive structure that is crucial to lung physiology.					
31248580	8	17	theme	rat	1447:1449	arg1	homogenates					1456:1466	the adult rat lung homogenates	1437:1466	the adult rat lung homogenates	1437:1466	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	6	18	theme	light	1025:1029	arg1	LM					1043:1044	LM	1043:1044	LM	1043:1044	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	6	18	theme	light	1025:1029	arg1	microscopy					1031:1040	light microscopy	1025:1040	light microscopy (LM) methods	1025:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	2	19	located	present	386:392	arg2	array					338:342	A dense array	330:342	A dense array of complex and diverse glycoconjugates	330:381	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	2	19	located	present	386:392	arg2	present					386:392	present	386:392	present	386:392	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	2	19	located	present	386:392	arg1	surfaces					425:432	essentially all animal cell surfaces	397:432	essentially all animal cell surfaces	397:432	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	4	20	theme	pathological	674:685	arg1	functions					687:695	many physiological and pathological functions	651:695	many physiological and pathological functions	651:695	Due to their abundance and their terminal position in glycans, sialic acids are implicated in many physiological and pathological functions.					
31248580	7	21	theme	liquid	1208:1213	arg1	HPLC					1231:1234	HPLC	1231:1234	HPLC	1231:1234	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	7	21	theme	liquid	1208:1213	arg1	chromatography					1215:1228	high-performance liquid chromatography	1191:1228	high-performance liquid chromatography (HPLC)	1191:1235	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	6	22	theme	epithelial	994:1003	arg1	surfaces					1010:1017	rat bronchioloalveolar epithelial cell surfaces	971:1017	rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	971:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	8	23	theme	2,73 μg/mg	1482:1491	arg1	prot.					1493:1497	2,73 μg/mg prot.	1482:1497	2,73 μg/mg prot.	1482:1497	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	0	24	theme	N-acetylneuraminic	94:111	arg1	acids					138:142	N-acetylneuraminic and N-glycolylneuraminic acids	94:142	N-acetylneuraminic and N-glycolylneuraminic acids	94:142	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	6	25	theme	rat	971:973	arg1	surfaces					1010:1017	rat bronchioloalveolar epithelial cell surfaces	971:1017	rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	971:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	8	26	theme	surface	1343:1349	arg1	coat					1351:1354	the surface coat	1339:1354	the surface coat of bronchioloalveolar cells	1339:1382	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	5	27	theme	glycans	755:761	arg1	composition					711:721	the composition	707:721	the composition of lung epithelial cell-surface glycans	707:761	Although the composition of lung epithelial cell-surface glycans has been studied over the years, it is not yet completely understood.					
31248580	8	28	theme	cells	1378:1382	arg1	coat					1351:1354	the surface coat	1339:1354	the surface coat of bronchioloalveolar cells	1339:1382	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	0	29	theme	sialic	12:17	arg1	acids					19:23	sialic acids	12:23	sialic acids	12:23	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	8	30	theme	acid	1307:1310	arg1	residues					1312:1319	Sialic acid residues	1300:1319	Sialic acid residues	1300:1319	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	9	31	theme	methodology	1759:1769	arg1	efficiency					1741:1750	the efficiency	1737:1750	the efficiency of the methodology	1737:1769	A coefficient of variation (CV) of 4,98% and 4,40%, respectively was obtained and an optimal dispersion variability expressed by the SD and the CV was also reported, confirming the efficiency of the methodology.					
31248580	7	32	dep	chromatography	1215:1228	arg1	means					1182:1186	means	1182:1186	means	1182:1186	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	2	33	theme	animal	413:418	arg1	surfaces					425:432	essentially all animal cell surfaces	397:432	essentially all animal cell surfaces	397:432	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	1	34	theme	specialized	300:310	arg1	cells					323:327	diverse and highly specialized epithelial cells	281:327	diverse and highly specialized epithelial cells	281:327	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	9	35	theme	variation	1577:1585	arg1	coefficient					1562:1572	A coefficient	1560:1572	A coefficient of variation (CV) of 4,98% and 4,40%, respectively	1560:1623	A coefficient of variation (CV) of 4,98% and 4,40%, respectively was obtained and an optimal dispersion variability expressed by the SD and the CV was also reported, confirming the efficiency of the methodology.					
31248580	11	36	theme	lung	2076:2079	arg1	physiology					2081:2090	lung physiology	2076:2090	lung physiology	2076:2090	The presence of these residues contributes to a strong anionic shield and may provide an hydrating and protective barrier as well as a repulsive structure that is crucial to lung physiology.					
31248580	8	37	theme	Neu5Ac	1552:1557	arg1	preponderance					1535:1547	preponderance	1535:1547	preponderance of Neu5Ac	1535:1557	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	8	38	theme	mean	1393:1396	arg1	12,26					1472:1476	12,26	1472:1476	12,26	1472:1476	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	8	38	theme	mean	1393:1396	arg1	quantification					1398:1411	the mean quantification	1389:1411	the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates	1389:1466	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	7	39	theme	optimized	1271:1279	arg1	methods					1286:1292	the optimized HPLC methods	1267:1292	the optimized HPLC methods used	1267:1297	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	1	40	dep	structure	212:220	arg1	composed					222:229	composed	222:229	composed of branching tubes ending in alveoli	222:266	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	1	40	dep	structure	212:220	arg1	lined					272:276	lined	272:276	lined by diverse and highly specialized epithelial cells	272:327	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	3	41	theme	outermost	476:484	arg1	ends					486:489	the outermost ends	472:489	the outermost ends of glycan chains, attached to membrane proteins and lipids below	472:554	Sialic acids are widely allocated at the outermost ends of glycan chains, attached to membrane proteins and lipids below.					
31248580	4	42	theme	terminal	590:597	arg1	position					599:606	their terminal position	584:606	their terminal position	584:606	Due to their abundance and their terminal position in glycans, sialic acids are implicated in many physiological and pathological functions.					
31248580	2	43	theme	all	409:411	arg1	surfaces					425:432	essentially all animal cell surfaces	397:432	essentially all animal cell surfaces	397:432	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	2	44	theme	glycoconjugates	367:381	arg1	array					338:342	A dense array	330:342	A dense array of complex and diverse glycoconjugates	330:381	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	2	44	theme	glycoconjugates	367:381	arg1	present					386:392	present	386:392	present	386:392	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	9	45	theme	%	1599:1599	arg1	coefficient					1562:1572	A coefficient	1560:1572	A coefficient of variation (CV) of 4,98% and 4,40%, respectively	1560:1623	A coefficient of variation (CV) of 4,98% and 4,40%, respectively was obtained and an optimal dispersion variability expressed by the SD and the CV was also reported, confirming the efficiency of the methodology.					
31248580	7	46	theme	high-performance	1191:1206	arg1	HPLC					1231:1234	HPLC	1231:1234	HPLC	1231:1234	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	7	46	theme	high-performance	1191:1206	arg1	chromatography					1215:1228	high-performance liquid chromatography	1191:1228	high-performance liquid chromatography (HPLC)	1191:1235	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	3	47	theme	chains	501:506	arg1	ends					486:489	the outermost ends	472:489	the outermost ends of glycan chains, attached to membrane proteins and lipids below	472:554	Sialic acids are widely allocated at the outermost ends of glycan chains, attached to membrane proteins and lipids below.					
31248580	8	48	theme	Neu5Gc	1427:1432	arg1	12,26					1472:1476	12,26	1472:1476	12,26	1472:1476	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	8	48	theme	Neu5Gc	1427:1432	arg1	quantification					1398:1411	the mean quantification	1389:1411	the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates	1389:1466	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	6	49	theme	acid	910:913	arg1	residues					959:966	N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues	891:966	N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	891:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	7	50	theme	lung	1098:1101	arg1	homogenates					1103:1113	adult rat lung homogenates	1088:1113	adult rat lung homogenates	1088:1113	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	7	51	theme	adult	1088:1092	arg1	homogenates					1103:1113	adult rat lung homogenates	1088:1113	adult rat lung homogenates	1088:1113	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	3	52	theme	membrane	521:528	arg1	proteins					530:537	membrane proteins	521:537	membrane proteins	521:537	Sialic acids are widely allocated at the outermost ends of glycan chains, attached to membrane proteins and lipids below.					
31248580	1	53	theme	air-breathing	170:182	arg1	vertebrates					184:194	air-breathing vertebrates	170:194	air-breathing vertebrates	170:194	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	4	54	theme	many	651:654	arg1	functions					687:695	many physiological and pathological functions	651:695	many physiological and pathological functions	651:695	Due to their abundance and their terminal position in glycans, sialic acids are implicated in many physiological and pathological functions.					
31248580	7	55	theme	lung	1059:1062	arg1	membranes					1064:1072	lung membranes	1059:1072	lung membranes isolated from adult rat lung homogenates	1059:1113	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	9	56	theme	optimal	1645:1651	arg1	variability					1664:1674	an optimal dispersion variability	1642:1674	an optimal dispersion variability expressed by the SD and the CV	1642:1705	A coefficient of variation (CV) of 4,98% and 4,40%, respectively was obtained and an optimal dispersion variability expressed by the SD and the CV was also reported, confirming the efficiency of the methodology.					
31248580	10	57	theme	sialic	1861:1866	arg1	acids					1868:1872	sialic acids	1861:1872	sialic acids	1861:1872	To the best of our knowledge, our group was the first to identify, separate and quantify sialic acids in purified lung membranes.					
31248580	8	58	from	quantification	1398:1411	arg1	homogenates					1456:1466	the adult rat lung homogenates	1437:1466	the adult rat lung homogenates	1437:1466	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	8	59	theme	adult	1441:1445	arg1	homogenates					1456:1466	the adult rat lung homogenates	1437:1466	the adult rat lung homogenates	1437:1466	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	0	60	from	Presence	0:7	arg1	lung					151:154	the lung	147:154	the lung	147:154	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	0	60	from	Presence	0:7	arg1	cells					47:51	bronchioloalveolar cells	28:51	bronchioloalveolar cells	28:51	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	6	61	theme	>N-glycolylneuraminic	923:943	arg1	residues					959:966	N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues	891:966	N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	891:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	8	62	theme	lung	1451:1454	arg1	homogenates					1456:1466	the adult rat lung homogenates	1437:1466	the adult rat lung homogenates	1437:1466	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	10	63	theme	lung	1886:1889	arg1	membranes					1891:1899	purified lung membranes	1877:1899	purified lung membranes	1877:1899	To the best of our knowledge, our group was the first to identify, separate and quantify sialic acids in purified lung membranes.					
31248580	6	64	theme	microscopy	1031:1040	arg1	methods					1047:1053	light microscopy (LM) methods	1025:1053	light microscopy (LM) methods	1025:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	2	65	theme	dense	332:336	arg1	array					338:342	A dense array	330:342	A dense array of complex and diverse glycoconjugates	330:381	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	2	65	theme	dense	332:336	arg1	present					386:392	present	386:392	present	386:392	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	8	66	theme	Sialic	1300:1305	arg1	residues					1312:1319	Sialic acid residues	1300:1319	Sialic acid residues	1300:1319	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	5	67	theme	epithelial	731:740	arg1	glycans					755:761	lung epithelial cell-surface glycans	726:761	lung epithelial cell-surface glycans	726:761	Although the composition of lung epithelial cell-surface glycans has been studied over the years, it is not yet completely understood.					
31248580	4	68	from	position	599:606	arg1	glycans					611:617	glycans	611:617	glycans	611:617	Due to their abundance and their terminal position in glycans, sialic acids are implicated in many physiological and pathological functions.					
31248580	6	69	theme	cell	1005:1008	arg1	surfaces					1010:1017	rat bronchioloalveolar epithelial cell surfaces	971:1017	rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	971:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	5	70	theme	lung	726:729	arg1	glycans					755:761	lung epithelial cell-surface glycans	726:761	lung epithelial cell-surface glycans	726:761	Although the composition of lung epithelial cell-surface glycans has been studied over the years, it is not yet completely understood.					
31248580	6	71	theme	bronchioloalveolar	975:992	arg1	surfaces					1010:1017	rat bronchioloalveolar epithelial cell surfaces	971:1017	rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	971:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	0	72	theme	acids	138:142	arg1	quantification					76:89	quantification	76:89	quantification	76:89	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	0	72	theme	acids	138:142	arg1	Presence					0:7	Presence	0:7	Presence of sialic acids in bronchioloalveolar cells	0:51	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	0	72	theme	acids	138:142	arg1	identification					57:70	identification	57:70	identification	57:70	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	1	73	from	lung	161:164	arg1	vertebrates					184:194	air-breathing vertebrates	170:194	air-breathing vertebrates	170:194	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	4	74	from	abundance	570:578	arg1	glycans					611:617	glycans	611:617	glycans	611:617	Due to their abundance and their terminal position in glycans, sialic acids are implicated in many physiological and pathological functions.					
31248580	3	75	theme	Sialic	435:440	arg1	acids					442:446	Sialic acids	435:446	Sialic acids	435:446	Sialic acids are widely allocated at the outermost ends of glycan chains, attached to membrane proteins and lipids below.					
31248580	0	76	theme	acids	19:23	arg1	quantification					76:89	quantification	76:89	quantification	76:89	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	0	76	theme	acids	19:23	arg1	Presence					0:7	Presence	0:7	Presence of sialic acids in bronchioloalveolar cells	0:51	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	0	76	theme	acids	19:23	arg1	identification					57:70	identification	57:70	identification	57:70	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	1	77	theme	diverse	281:287	arg1	cells					323:327	diverse and highly specialized epithelial cells	281:327	diverse and highly specialized epithelial cells	281:327	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	8	78	theme	bronchioloalveolar	1359:1376	arg1	cells					1378:1382	bronchioloalveolar cells	1359:1382	bronchioloalveolar cells	1359:1382	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	2	79	theme	cell	420:423	arg1	surfaces					425:432	essentially all animal cell surfaces	397:432	essentially all animal cell surfaces	397:432	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	0	80	theme	bronchioloalveolar	28:45	arg1	cells					47:51	bronchioloalveolar cells	28:51	bronchioloalveolar cells	28:51	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	7	81	theme	HPLC	1281:1284	arg1	methods					1286:1292	the optimized HPLC methods	1267:1292	the optimized HPLC methods used	1267:1297	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	6	82	from	residues	959:966	arg1	surfaces					1010:1017	rat bronchioloalveolar epithelial cell surfaces	971:1017	rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods	971:1053	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	0	83	theme	N-glycolylneuraminic	117:136	arg1	acids					138:142	N-acetylneuraminic and N-glycolylneuraminic acids	94:142	N-acetylneuraminic and N-glycolylneuraminic acids	94:142	Presence of sialic acids in bronchioloalveolar cells and identification and quantification of N-acetylneuraminic and N-glycolylneuraminic acids in the lung.					
31248580	1	84	theme	epithelial	312:321	arg1	cells					323:327	diverse and highly specialized epithelial cells	281:327	diverse and highly specialized epithelial cells	281:327	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	6	85	theme	present	840:846	arg1	work					848:851	the present work	836:851	the present work	836:851	In the present work, we aimed to histochemically localize N-acetylneuraminic acid (Neu5Ac)>N-glycolylneuraminic acid (Neu5Gc) residues on rat bronchioloalveolar epithelial cell surfaces using light microscopy (LM) methods.					
31248580	3	86	attach	attached	509:516	arg1	lipids					543:548	lipids	543:548	lipids	543:548	Sialic acids are widely allocated at the outermost ends of glycan chains, attached to membrane proteins and lipids below.					
31248580	3	86	attach	attached	509:516	arg2	chains					501:506	glycan chains	494:506	glycan chains	494:506	Sialic acids are widely allocated at the outermost ends of glycan chains, attached to membrane proteins and lipids below.					
31248580	3	86	attach	attached	509:516	arg1	proteins					530:537	membrane proteins	521:537	membrane proteins	521:537	Sialic acids are widely allocated at the outermost ends of glycan chains, attached to membrane proteins and lipids below.					
31248580	3	87	theme	glycan	494:499	arg1	chains					501:506	glycan chains	494:506	glycan chains	494:506	Sialic acids are widely allocated at the outermost ends of glycan chains, attached to membrane proteins and lipids below.					
31248580	5	88	theme	cell-surface	742:753	arg1	glycans					755:761	lung epithelial cell-surface glycans	726:761	lung epithelial cell-surface glycans	726:761	Although the composition of lung epithelial cell-surface glycans has been studied over the years, it is not yet completely understood.					
31248580	1	89	theme	branching	234:242	arg1	tubes					244:248	branching tubes	234:248	branching tubes ending in alveoli	234:266	The lung, in air-breathing vertebrates, is a tree-like structure composed of branching tubes ending in alveoli and lined by diverse and highly specialized epithelial cells.					
31248580	8	90	located	localized	1326:1334	arg2	residues					1312:1319	Sialic acid residues	1300:1319	Sialic acid residues	1300:1319	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	8	90	located	localized	1326:1334	arg1	coat					1351:1354	the surface coat	1339:1354	the surface coat of bronchioloalveolar cells	1339:1382	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	2	91	attach	present	386:392	arg1	surfaces					425:432	essentially all animal cell surfaces	397:432	essentially all animal cell surfaces	397:432	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	2	91	attach	present	386:392	arg2	present					386:392	present	386:392	present	386:392	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	2	91	attach	present	386:392	arg2	array					338:342	A dense array	330:342	A dense array of complex and diverse glycoconjugates	330:381	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31248580	7	92	attach	isolated	1074:1081	arg2	membranes					1064:1072	lung membranes	1059:1072	lung membranes isolated from adult rat lung homogenates	1059:1113	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	7	92	attach	isolated	1074:1081	arg1	homogenates					1103:1113	adult rat lung homogenates	1088:1113	adult rat lung homogenates	1088:1113	In lung membranes isolated from adult rat lung homogenates, we also separated, identified and quantified Neu5Ac and Neu5Gc by means of high-performance liquid chromatography (HPLC), and systematically described the optimized HPLC methods used.					
31248580	8	93	theme	Neu5Ac	1416:1421	arg1	12,26					1472:1476	12,26	1472:1476	12,26	1472:1476	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	8	93	theme	Neu5Ac	1416:1421	arg1	quantification					1398:1411	the mean quantification	1389:1411	the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates	1389:1466	Sialic acid residues were localized on the surface coat of bronchioloalveolar cells, and the mean quantification of Neu5Ac and Neu5Gc in the adult rat lung homogenates was 12,26 and 2,73 μg/mg prot., respectively, revealing a manifest preponderance of Neu5Ac.					
31248580	2	94	theme	diverse	359:365	arg1	glycoconjugates					367:381	complex and diverse glycoconjugates	347:381	complex and diverse glycoconjugates	347:381	A dense array of complex and diverse glycoconjugates is present on essentially all animal cell surfaces.					
31038500	4	0	theme	size	965:968	arg1	distribution					970:981	narrow size distribution	958:981	narrow size distribution	958:981	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	3	1	theme	surface	787:793	arg1	layer					795:799	a hyaluronic acid surface layer	769:799	a hyaluronic acid surface layer	769:799	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	6	2	theme	mice	1593:1596	arg1	model					1598:1602	a MCF-7 tumor-bearing mice model	1571:1602	a MCF-7 tumor-bearing mice model	1571:1602	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	3	3	theme	coordination	830:841	arg1	kinetics					852:859	coordination reaction kinetics	830:859	coordination reaction kinetics	830:859	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	3	4	theme	flash	637:641	arg1	process					667:673	a coordination-driven flash nanocomplexation (cFNC) process	615:673	a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing	615:887	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	1	5	theme	single	306:311	arg1	nanoparticle					328:339	a single multicomponent nanoparticle	304:339	a single multicomponent nanoparticle via a simple and scalable approach	304:374	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	2	6	from	bottlenecks	391:401	arg1	capability					488:497	drug loading capability	475:497	drug loading capability	475:497	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	2	6	from	bottlenecks	391:401	arg1	repeatability					518:530	batch-to-batch repeatability	503:530	batch-to-batch repeatability	503:530	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	2	6	from	bottlenecks	391:401	arg1	controllability					437:451	composition controllability	425:451	composition controllability	425:451	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	2	6	from	bottlenecks	391:401	arg1	stability					464:472	colloidal stability	454:472	colloidal stability	454:472	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	3	7	theme	kinetics	852:859	arg1	control					819:825	efficient control	809:825	efficient control of coordination reaction kinetics	809:859	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	5	8	theme	CD44+	1171:1175	arg1	targeting					1186:1194	CD44+ receptor targeting	1171:1194	CD44+ receptor targeting	1171:1194	The studies indicated that DITH significantly increased cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli.					
31038500	1	9	theme	effective	234:242	arg1	integration					244:254	effective integration	234:254	effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach	234:374	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	3	10	theme	scalable	686:693	arg1	fabrication					695:705	scalable fabrication	686:705	scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer	686:799	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	4	11	theme	drug	989:992	arg1	capacity					1002:1009	high drug loading capacity	984:1009	high drug loading capacity (26.6%)	984:1017	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	4	11	theme	drug	989:992	arg1	%					1016:1016	26.6%	1012:1016	26.6%	1012:1016	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	3	12	theme	turbulent	872:880	arg1	mixing					882:887	a rapid turbulent mixing	864:887	a rapid turbulent mixing	864:887	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	6	13	theme	prolonged	1416:1424	arg1	circulation					1432:1442	prolonged blood circulation	1416:1442	prolonged blood circulation	1416:1442	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	5	14	theme	cellular	1138:1145	arg1	endocytosis					1147:1157	cellular endocytosis	1138:1157	cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli	1138:1298	The studies indicated that DITH significantly increased cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli.					
31038500	7	15	theme	new	1737:1739	arg1	strategy					1741:1748	a new strategy	1735:1748	a new strategy to promote the development of translational nanomedicine	1735:1805	These results confirmed that the developed DITH is a promising vehicle for cancer theranostic applications, and our work provided a new strategy to promote the development of translational nanomedicine.					
31038500	6	16	theme	T1-weighted	1324:1334	arg1	resonance					1345:1353	T1-weighted magnetic resonance	1324:1353	T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions	1324:1398	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	6	16	theme	T1-weighted	1324:1334	arg1	MR					1356:1357	MR	1356:1357	MR	1356:1357	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	3	17	theme	metal-phenolic	728:741	arg1	DITH					758:761	DITH	758:761	DITH	758:761	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	3	17	theme	metal-phenolic	728:741	arg1	nanoparticles					743:755	doxorubicin-based metal-phenolic nanoparticles	710:755	doxorubicin-based metal-phenolic nanoparticles (DITH)	710:762	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	4	18	theme	drug	1058:1061	arg1	behaviors					1071:1079	pH-triggered drug release behaviors	1045:1079	pH-triggered drug release behaviors	1045:1079	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	0	19	theme	cancer	103:108	arg1	theranostics					110:121	cancer theranostics	103:121	cancer theranostics	103:121	Scalable fabrication of metal-phenolic nanoparticles by coordination-driven flash nanocomplexation for cancer theranostics.					
31038500	4	20	theme	high	1020:1023	arg1	reproducibility					1025:1039	high reproducibility	1020:1039	high reproducibility	1020:1039	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	5	21	theme	accelerated	1200:1210	arg1	release					1231:1237	accelerated intracellular drug release	1200:1237	accelerated intracellular drug release	1200:1237	The studies indicated that DITH significantly increased cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli.					
31038500	7	22	theme	theranostic	1687:1697	arg1	applications					1699:1710	cancer theranostic applications	1680:1710	cancer theranostic applications	1680:1710	These results confirmed that the developed DITH is a promising vehicle for cancer theranostic applications, and our work provided a new strategy to promote the development of translational nanomedicine.					
31038500	2	23	theme	colloidal	454:462	arg1	stability					464:472	colloidal stability	454:472	colloidal stability	454:472	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	5	24	theme	drug	1226:1229	arg1	release					1231:1237	accelerated intracellular drug release	1200:1237	accelerated intracellular drug release	1200:1237	The studies indicated that DITH significantly increased cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli.					
31038500	3	25	with	fabrication	695:705	arg1	layer					795:799	a hyaluronic acid surface layer	769:799	a hyaluronic acid surface layer	769:799	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	0	26	theme	Scalable	0:7	arg1	fabrication					9:19	Scalable fabrication	0:19	Scalable fabrication of metal-phenolic nanoparticles by coordination-driven flash nanocomplexation for cancer theranostics.	0:122	Scalable fabrication of metal-phenolic nanoparticles by coordination-driven flash nanocomplexation for cancer theranostics.					
31038500	1	27	theme	multicomponent	313:326	arg1	nanoparticle					328:339	a single multicomponent nanoparticle	304:339	a single multicomponent nanoparticle via a simple and scalable approach	304:374	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	2	28	theme	loading	480:486	arg1	capability					488:497	drug loading capability	475:497	drug loading capability	475:497	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	7	29	theme	translational	1780:1792	arg1	nanomedicine					1794:1805	translational nanomedicine	1780:1805	translational nanomedicine	1780:1805	These results confirmed that the developed DITH is a promising vehicle for cancer theranostic applications, and our work provided a new strategy to promote the development of translational nanomedicine.					
31038500	4	30	theme	hydrodynamic	927:938	arg1	diameter					940:947	a small hydrodynamic diameter	919:947	a small hydrodynamic diameter (84 nm)	919:955	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	4	30	theme	hydrodynamic	927:938	arg1	nm					953:954	84 nm	950:954	84 nm	950:954	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	0	31	theme	nanoparticles	39:51	arg1	fabrication					9:19	Scalable fabrication	0:19	Scalable fabrication of metal-phenolic nanoparticles by coordination-driven flash nanocomplexation for cancer theranostics.	0:122	Scalable fabrication of metal-phenolic nanoparticles by coordination-driven flash nanocomplexation for cancer theranostics.					
31038500	1	32	theme	simple	347:352	arg1	approach					367:374	a simple and scalable approach	345:374	a simple and scalable approach	345:374	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	6	33	theme	side	1527:1530	arg1	effects					1532:1538	toxic side effects	1521:1538	toxic side effects	1521:1538	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	1	34	theme	scalable	358:365	arg1	approach					367:374	a simple and scalable approach	345:374	a simple and scalable approach	345:374	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	3	35	theme	acid	782:785	arg1	layer					795:799	a hyaluronic acid surface layer	769:799	a hyaluronic acid surface layer	769:799	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	1	36	theme	therapeutic	259:269	arg1	drugs					271:275	therapeutic drugs	259:275	therapeutic drugs	259:275	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	3	37	theme	efficient	809:817	arg1	control					819:825	efficient control	809:825	efficient control of coordination reaction kinetics	809:859	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	1	38	theme	agents	292:297	arg1	integration					244:254	effective integration	234:254	effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach	234:374	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	6	39	dep	function	1368:1375	arg1	endowed					1377:1383	endowed	1377:1383	endowed	1377:1383	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	4	40	theme	narrow	958:963	arg1	distribution					970:981	narrow size distribution	958:981	narrow size distribution	958:981	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	6	41	theme	ferric	1388:1393	arg1	ions					1395:1398	ferric ions	1388:1398	ferric ions	1388:1398	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	3	42	theme	coordination-driven	617:635	arg1	process					667:673	a coordination-driven flash nanocomplexation (cFNC) process	615:673	a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing	615:887	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	6	43	theme	tumor-bearing	1579:1591	arg1	model					1598:1602	a MCF-7 tumor-bearing mice model	1571:1602	a MCF-7 tumor-bearing mice model	1571:1602	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	3	44	theme	reaction	843:850	arg1	kinetics					852:859	coordination reaction kinetics	830:859	coordination reaction kinetics	830:859	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	0	45	theme	coordination-driven	56:74	arg1	nanocomplexation					82:97	coordination-driven flash nanocomplexation	56:97	coordination-driven flash nanocomplexation for cancer theranostics	56:121	Scalable fabrication of metal-phenolic nanoparticles by coordination-driven flash nanocomplexation for cancer theranostics.					
31038500	2	46	theme	nanomedicine	583:594	arg1	translation					568:578	the clinical translation	555:578	the clinical translation of nanomedicine	555:594	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	2	47	theme	clinical	559:566	arg1	translation					568:578	the clinical translation	555:578	the clinical translation of nanomedicine	555:594	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	3	48	theme	cFNC	661:664	arg1	process					667:673	a coordination-driven flash nanocomplexation (cFNC) process	615:673	a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing	615:887	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	1	49	theme	key	216:218	arg1	challenge					220:228	a key challenge	214:228	a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach	214:374	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	3	50	theme	rapid	866:870	arg1	mixing					882:887	a rapid turbulent mixing	864:887	a rapid turbulent mixing	864:887	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	6	51	theme	tumor	1454:1458	arg1	accumulation					1460:1471	tumor accumulation	1454:1471	tumor accumulation	1454:1471	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	3	52	theme	nanocomplexation	643:658	arg1	process					667:673	a coordination-driven flash nanocomplexation (cFNC) process	615:673	a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing	615:887	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	5	53	theme	receptor	1177:1184	arg1	targeting					1186:1194	CD44+ receptor targeting	1171:1194	CD44+ receptor targeting	1171:1194	The studies indicated that DITH significantly increased cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli.					
31038500	6	54	from	injection	1558:1566	arg1	model					1598:1602	a MCF-7 tumor-bearing mice model	1571:1602	a MCF-7 tumor-bearing mice model	1571:1602	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	6	55	theme	blood	1426:1430	arg1	circulation					1432:1442	prolonged blood circulation	1416:1442	prolonged blood circulation	1416:1442	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	4	56	theme	high	984:987	arg1	capacity					1002:1009	high drug loading capacity	984:1009	high drug loading capacity (26.6%)	984:1017	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	4	56	theme	high	984:987	arg1	%					1016:1016	26.6%	1012:1016	26.6%	1012:1016	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	6	57	theme	magnetic	1336:1343	arg1	resonance					1345:1353	T1-weighted magnetic resonance	1324:1353	T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions	1324:1398	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	6	57	theme	magnetic	1336:1343	arg1	MR					1356:1357	MR	1356:1357	MR	1356:1357	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	3	58	theme	doxorubicin-based	710:726	arg1	DITH					758:761	DITH	758:761	DITH	758:761	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	3	58	theme	doxorubicin-based	710:726	arg1	nanoparticles					743:755	doxorubicin-based metal-phenolic nanoparticles	710:755	doxorubicin-based metal-phenolic nanoparticles (DITH)	710:762	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	4	59	theme	loading	994:1000	arg1	capacity					1002:1009	high drug loading capacity	984:1009	high drug loading capacity (26.6%)	984:1017	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	4	59	theme	loading	994:1000	arg1	%					1016:1016	26.6%	1012:1016	26.6%	1012:1016	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	6	60	theme	toxic	1521:1525	arg1	effects					1532:1538	toxic side effects	1521:1538	toxic side effects	1521:1538	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	1	61	theme	cancer	179:184	arg1	theranostics					186:197	cancer theranostics	179:197	cancer theranostics	179:197	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	4	62	theme	pH-triggered	1045:1056	arg1	behaviors					1071:1079	pH-triggered drug release behaviors	1045:1079	pH-triggered drug release behaviors	1045:1079	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	4	63	theme	release	1063:1069	arg1	behaviors					1071:1079	pH-triggered drug release behaviors	1045:1079	pH-triggered drug release behaviors	1045:1079	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	6	64	theme	therapeutic	1483:1493	arg1	performance					1495:1505	therapeutic performance	1483:1505	therapeutic performance	1483:1505	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	7	65	theme	cancer	1680:1685	arg1	applications					1699:1710	cancer theranostic applications	1680:1710	cancer theranostic applications	1680:1710	These results confirmed that the developed DITH is a promising vehicle for cancer theranostic applications, and our work provided a new strategy to promote the development of translational nanomedicine.					
31038500	5	66	theme	intracellular	1212:1224	arg1	release					1231:1237	accelerated intracellular drug release	1200:1237	accelerated intracellular drug release	1200:1237	The studies indicated that DITH significantly increased cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli.					
31038500	6	67	theme	MCF-7	1573:1577	arg1	model					1598:1602	a MCF-7 tumor-bearing mice model	1571:1602	a MCF-7 tumor-bearing mice model	1571:1602	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	2	68	theme	batch-to-batch	503:516	arg1	repeatability					518:530	batch-to-batch repeatability	503:530	batch-to-batch repeatability	503:530	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	2	69	theme	composition	425:435	arg1	controllability					437:451	composition controllability	425:451	composition controllability	425:451	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	0	70	theme	metal-phenolic	24:37	arg1	nanoparticles					39:51	metal-phenolic nanoparticles	24:51	metal-phenolic nanoparticles	24:51	Scalable fabrication of metal-phenolic nanoparticles by coordination-driven flash nanocomplexation for cancer theranostics.					
31038500	1	71	theme	various	133:139	arg1	nanomaterials					141:153	various nanomaterials	133:153	various nanomaterials	133:153	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	2	72	theme	drug	475:478	arg1	capability					488:497	drug loading capability	475:497	drug loading capability	475:497	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	6	73	theme	intravenous	1546:1556	arg1	injection					1558:1566	intravenous injection	1546:1566	intravenous injection in a MCF-7 tumor-bearing mice model	1546:1602	Furthermore, guided by T1-weighted magnetic resonance (MR) imaging function endowed by ferric ions, DITH exhibited prolonged blood circulation, enhanced tumor accumulation, improved therapeutic performance and decreased toxic side effects after intravenous injection in a MCF-7 tumor-bearing mice model.					
31038500	7	74	theme	nanomedicine	1794:1805	arg1	development					1765:1775	the development	1761:1775	the development of translational nanomedicine	1761:1805	These results confirmed that the developed DITH is a promising vehicle for cancer theranostic applications, and our work provided a new strategy to promote the development of translational nanomedicine.					
31038500	3	75	theme	nanoparticles	743:755	arg1	fabrication					695:705	scalable fabrication	686:705	scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer	686:799	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	4	76	theme	small	921:925	arg1	diameter					940:947	a small hydrodynamic diameter	919:947	a small hydrodynamic diameter (84 nm)	919:955	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	4	76	theme	small	921:925	arg1	nm					953:954	84 nm	950:954	84 nm	950:954	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	0	77	theme	flash	76:80	arg1	nanocomplexation					82:97	coordination-driven flash nanocomplexation	56:97	coordination-driven flash nanocomplexation for cancer theranostics	56:121	Scalable fabrication of metal-phenolic nanoparticles by coordination-driven flash nanocomplexation for cancer theranostics.					
31038500	5	78	theme	DITH	1267:1270	arg1	sensitivity					1252:1262	the sensitivity	1248:1262	the sensitivity of DITH to environmental pH stimuli	1248:1298	The studies indicated that DITH significantly increased cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli.					
31038500	7	79	theme	promising	1658:1666	arg1	DITH					1648:1651	the developed DITH	1634:1651	the developed DITH	1634:1651	These results confirmed that the developed DITH is a promising vehicle for cancer theranostic applications, and our work provided a new strategy to promote the development of translational nanomedicine.					
31038500	7	79	theme	promising	1658:1666	arg1	vehicle					1668:1674	a promising vehicle	1656:1674	a promising vehicle for cancer theranostic applications	1656:1710	These results confirmed that the developed DITH is a promising vehicle for cancer theranostic applications, and our work provided a new strategy to promote the development of translational nanomedicine.					
31038500	3	80	theme	hyaluronic	771:780	arg1	layer					795:799	a hyaluronic acid surface layer	769:799	a hyaluronic acid surface layer	769:799	Herein, we report a coordination-driven flash nanocomplexation (cFNC) process to achieve scalable fabrication of doxorubicin-based metal-phenolic nanoparticles (DITH) with a hyaluronic acid surface layer through efficient control of coordination reaction kinetics in a rapid turbulent mixing.					
31038500	5	81	theme	environmental	1275:1287	arg1	stimuli					1292:1298	environmental pH stimuli	1275:1298	environmental pH stimuli	1275:1298	The studies indicated that DITH significantly increased cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli.					
31038500	4	82	theme	optimized	894:902	arg1	DITH					904:907	The optimized DITH	890:907	The optimized DITH	890:907	The optimized DITH exhibited a small hydrodynamic diameter (84 nm), narrow size distribution, high drug loading capacity (26.6%), high reproducibility and pH-triggered drug release behaviors.					
31038500	1	83	theme	drugs	271:275	arg1	integration					244:254	effective integration	234:254	effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach	234:374	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
31038500	7	84	theme	developed	1638:1646	arg1	DITH					1648:1651	the developed DITH	1634:1651	the developed DITH	1634:1651	These results confirmed that the developed DITH is a promising vehicle for cancer theranostic applications, and our work provided a new strategy to promote the development of translational nanomedicine.					
31038500	7	84	theme	developed	1638:1646	arg1	vehicle					1668:1674	a promising vehicle	1656:1674	a promising vehicle for cancer theranostic applications	1656:1710	These results confirmed that the developed DITH is a promising vehicle for cancer theranostic applications, and our work provided a new strategy to promote the development of translational nanomedicine.					
31038500	5	85	theme	pH	1289:1290	arg1	stimuli					1292:1298	environmental pH stimuli	1275:1298	environmental pH stimuli	1275:1298	The studies indicated that DITH significantly increased cellular endocytosis mediated by CD44+ receptor targeting and accelerated intracellular drug release owing to the sensitivity of DITH to environmental pH stimuli.					
31038500	2	86	theme	nanoformulation	406:420	arg1	bottlenecks					391:401	the bottlenecks	387:401	the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability	387:530	Moreover, the bottlenecks of nanoformulation in composition controllability, colloidal stability, drug loading capability and batch-to-batch repeatability currently still hinder the clinical translation of nanomedicine.					
31038500	1	87	theme	diagnostic	281:290	arg1	agents					292:297	diagnostic agents	281:297	diagnostic agents	281:297	Although various nanomaterials have been developed for cancer theranostics, there remains a key challenge for effective integration of therapeutic drugs and diagnostic agents into a single multicomponent nanoparticle via a simple and scalable approach.					
30901847	9	0	theme	fermented	1184:1192	arg1	wheat					1194:1198	The fermented wheat	1180:1198	The fermented wheat	1180:1198	The fermented wheat was a suitable substrate for the grown of L. reuteri, recognized for its health properties in the human gut.					
30901847	9	0	theme	fermented	1184:1192	arg1	substrate					1215:1223	a suitable substrate	1204:1223	a suitable substrate	1204:1223	The fermented wheat was a suitable substrate for the grown of L. reuteri, recognized for its health properties in the human gut.					
30901847	3	1	from	composition	373:383	arg1	PTP1B					435:439	PTP1B	435:439	PTP1B	435:439	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	1	from	composition	373:383	arg1	1B					431:432	protein tyrosine phosphatase 1B	402:432	protein tyrosine phosphatase 1B (PTP1B)	402:440	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	1	from	composition	373:383	arg1	reuteri					539:545	Lactobacillus reuteri	525:545	Lactobacillus reuteri	525:545	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	1	from	composition	373:383	arg1	enzyme					446:451	an enzyme	443:451	an enzyme overexpressed in type-II diabetes	443:485	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	10	2	theme	fermented	1519:1527	arg1	cereals					1529:1535	fermented cereals	1519:1535	fermented cereals	1519:1535	The proposed method for phenols is easier compared to those based on strong basic hydrolysis; our results assessed the bound phenols as the major fraction, differently from that suggested by the literature for fermented cereals.					
30901847	6	3	theme	extracts	829:836	arg1	power					816:820	The inhibitory power	801:820	The inhibitory power of the extracts of several batches	801:855	The inhibitory power of the extracts of several batches were investigated on PTP1B enzyme.					
30901847	1	4	theme	staple	131:136	arg1	cereals					122:128	Fermented cereals	112:128	Fermented cereals	112:128	Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries.					
30901847	1	4	theme	staple	131:136	arg1	foods					138:142	staple foods	131:142	staple foods in Asia and Africa	131:161	Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries.					
30901847	10	5	theme	bound	1428:1432	arg1	phenols					1434:1440	the bound phenols	1424:1440	the bound phenols as the major fraction	1424:1462	The proposed method for phenols is easier compared to those based on strong basic hydrolysis; our results assessed the bound phenols as the major fraction, differently from that suggested by the literature for fermented cereals.					
30901847	1	6	theme	Western	209:215	arg1	countries					217:225	Western countries	209:225	Western countries	209:225	Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries.					
30901847	0	7	from	Study	0:4	arg1	Wheat					27:31	a Fermented Whole Wheat	9:31	a Fermented Whole Wheat	9:31	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	10	8	theme	proposed	1313:1320	arg1	method					1322:1327	The proposed method	1309:1327	The proposed method for phenols	1309:1339	The proposed method for phenols is easier compared to those based on strong basic hydrolysis; our results assessed the bound phenols as the major fraction, differently from that suggested by the literature for fermented cereals.					
30901847	10	8	theme	proposed	1313:1320	arg1	easier					1344:1349	easier	1344:1349	easier compared to those based on strong basic hydrolysis	1344:1400	The proposed method for phenols is easier compared to those based on strong basic hydrolysis; our results assessed the bound phenols as the major fraction, differently from that suggested by the literature for fermented cereals.					
30901847	0	9	dep	In	81:82	arg1	Vitro					84:88	Vitro	84:88	Vitro	84:88	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	4	10	theme	phenols	672:678	arg1	recovery					654:661	an exhaustive recovery	640:661	an exhaustive recovery of bound phenols	640:678	Basic and acidic hydrolysis were tested for an exhaustive recovery of bound phenols: the acidic hydrolysis gave best yields.					
30901847	0	11	theme	PTP1B	64:68	arg1	Enzyme					70:75	PTP1B Enzyme	64:75	PTP1B Enzyme	64:75	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	3	12	from	composition	565:575	arg1	PTP1B					435:439	PTP1B	435:439	PTP1B	435:439	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	12	from	composition	565:575	arg1	1B					431:432	protein tyrosine phosphatase 1B	402:432	protein tyrosine phosphatase 1B (PTP1B)	402:440	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	12	from	composition	565:575	arg1	reuteri					539:545	Lactobacillus reuteri	525:545	Lactobacillus reuteri	525:545	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	12	from	composition	565:575	arg1	enzyme					446:451	an enzyme	443:451	an enzyme overexpressed in type-II diabetes	443:485	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	4	13	theme	exhaustive	643:652	arg1	recovery					654:661	an exhaustive recovery	640:661	an exhaustive recovery of bound phenols	640:678	Basic and acidic hydrolysis were tested for an exhaustive recovery of bound phenols: the acidic hydrolysis gave best yields.					
30901847	0	14	theme	In	81:82	arg1	Properties					100:109	In Vitro Prebiotic Properties	81:109	In Vitro Prebiotic Properties	81:109	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	3	15	theme	phosphatase	419:429	arg1	PTP1B					435:439	PTP1B	435:439	PTP1B	435:439	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	15	theme	phosphatase	419:429	arg1	1B					431:432	protein tyrosine phosphatase 1B	402:432	protein tyrosine phosphatase 1B (PTP1B)	402:440	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	15	theme	phosphatase	419:429	arg1	enzyme					446:451	an enzyme	443:451	an enzyme overexpressed in type-II diabetes	443:485	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	8	16	theme	microbial	1057:1065	arg1	composition					1067:1077	The microbial composition	1053:1077	The microbial composition	1053:1077	The microbial composition was assessed identifying Lactobacillus, Enterococcus, and Pediococcus as the main bacterial species.					
30901847	8	17	theme	main	1156:1159	arg1	Enterococcus					1119:1130	Enterococcus	1119:1130	Enterococcus	1119:1130	The microbial composition was assessed identifying Lactobacillus, Enterococcus, and Pediococcus as the main bacterial species.					
30901847	8	17	theme	main	1156:1159	arg1	species					1171:1177	the main bacterial species	1152:1177	the main bacterial species	1152:1177	The microbial composition was assessed identifying Lactobacillus, Enterococcus, and Pediococcus as the main bacterial species.					
30901847	8	17	theme	main	1156:1159	arg1	Pediococcus					1137:1147	Pediococcus	1137:1147	Pediococcus	1137:1147	The microbial composition was assessed identifying Lactobacillus, Enterococcus, and Pediococcus as the main bacterial species.					
30901847	8	17	theme	main	1156:1159	arg1	Lactobacillus					1104:1116	Lactobacillus	1104:1116	Lactobacillus	1104:1116	The microbial composition was assessed identifying Lactobacillus, Enterococcus, and Pediococcus as the main bacterial species.					
30901847	3	18	theme	phenolic	364:371	arg1	composition					373:383	the phenolic composition	360:383	the phenolic composition	360:383	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	7	19	theme	major	1025:1029	arg1	C-flavonoid					1031:1041	a major C-flavonoid	1023:1041	a major C-flavonoid of wheat	1023:1050	The product was not able to inhibit the enzyme, otherwise, for the first time, a complete inhibition was observed for schaftoside, a major C-flavonoid of wheat.					
30901847	7	19	theme	major	1025:1029	arg1	schaftoside					1010:1020	schaftoside	1010:1020	schaftoside	1010:1020	The product was not able to inhibit the enzyme, otherwise, for the first time, a complete inhibition was observed for schaftoside, a major C-flavonoid of wheat.					
30901847	6	20	theme	inhibitory	805:814	arg1	power					816:820	The inhibitory power	801:820	The inhibitory power of the extracts of several batches	801:855	The inhibitory power of the extracts of several batches were investigated on PTP1B enzyme.					
30901847	9	21	theme	health	1273:1278	arg1	properties					1280:1289	its health properties	1269:1289	its health properties in the human gut	1269:1306	The fermented wheat was a suitable substrate for the grown of L. reuteri, recognized for its health properties in the human gut.					
30901847	2	22	theme	dietary	327:333	arg1	supplement					335:344	dietary supplement	327:344	dietary supplement	327:344	The object of this work is the characterization of a fermented wheat used as a food ingredient and dietary supplement.					
30901847	4	23	theme	bound	666:670	arg1	phenols					672:678	bound phenols	666:678	bound phenols	666:678	Basic and acidic hydrolysis were tested for an exhaustive recovery of bound phenols: the acidic hydrolysis gave best yields.					
30901847	9	24	theme	suitable	1206:1213	arg1	wheat					1194:1198	The fermented wheat	1180:1198	The fermented wheat	1180:1198	The fermented wheat was a suitable substrate for the grown of L. reuteri, recognized for its health properties in the human gut.					
30901847	9	24	theme	suitable	1206:1213	arg1	substrate					1215:1223	a suitable substrate	1204:1223	a suitable substrate	1204:1223	The fermented wheat was a suitable substrate for the grown of L. reuteri, recognized for its health properties in the human gut.					
30901847	7	25	theme	complete	973:980	arg1	inhibition					982:991	a complete inhibition	971:991	a complete inhibition	971:991	The product was not able to inhibit the enzyme, otherwise, for the first time, a complete inhibition was observed for schaftoside, a major C-flavonoid of wheat.					
30901847	4	26	theme	acidic	685:690	arg1	hydrolysis					692:701	the acidic hydrolysis	681:701	the acidic hydrolysis	681:701	Basic and acidic hydrolysis were tested for an exhaustive recovery of bound phenols: the acidic hydrolysis gave best yields.					
30901847	4	27	theme	Basic	596:600	arg1	hydrolysis					613:622	Basic and acidic hydrolysis	596:622	Basic and acidic hydrolysis	596:622	Basic and acidic hydrolysis were tested for an exhaustive recovery of bound phenols: the acidic hydrolysis gave best yields.					
30901847	0	28	theme	Fermented	11:19	arg1	Wheat					27:31	a Fermented Whole Wheat	9:31	a Fermented Whole Wheat	9:31	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	0	29	theme	Prebiotic	90:98	arg1	Properties					100:109	In Vitro Prebiotic Properties	81:109	In Vitro Prebiotic Properties	81:109	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	3	30	dep	in	492:493	arg1	vitro					495:499	vitro	495:499	vitro	495:499	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	31	theme	type-II	470:476	arg1	diabetes					478:485	type-II diabetes	470:485	type-II diabetes	470:485	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	8	32	theme	bacterial	1161:1169	arg1	Enterococcus					1119:1130	Enterococcus	1119:1130	Enterococcus	1119:1130	The microbial composition was assessed identifying Lactobacillus, Enterococcus, and Pediococcus as the main bacterial species.					
30901847	8	32	theme	bacterial	1161:1169	arg1	species					1171:1177	the main bacterial species	1152:1177	the main bacterial species	1152:1177	The microbial composition was assessed identifying Lactobacillus, Enterococcus, and Pediococcus as the main bacterial species.					
30901847	8	32	theme	bacterial	1161:1169	arg1	Pediococcus					1137:1147	Pediococcus	1137:1147	Pediococcus	1137:1147	The microbial composition was assessed identifying Lactobacillus, Enterococcus, and Pediococcus as the main bacterial species.					
30901847	8	32	theme	bacterial	1161:1169	arg1	Lactobacillus					1104:1116	Lactobacillus	1104:1116	Lactobacillus	1104:1116	The microbial composition was assessed identifying Lactobacillus, Enterococcus, and Pediococcus as the main bacterial species.					
30901847	10	33	theme	major	1449:1453	arg1	fraction					1455:1462	the major fraction	1445:1462	the major fraction	1445:1462	The proposed method for phenols is easier compared to those based on strong basic hydrolysis; our results assessed the bound phenols as the major fraction, differently from that suggested by the literature for fermented cereals.					
30901847	2	34	theme	work	247:250	arg1	object					232:237	The object	228:237	The object of this work	228:250	The object of this work is the characterization of a fermented wheat used as a food ingredient and dietary supplement.					
30901847	2	34	theme	work	247:250	arg1	characterization					259:274	the characterization	255:274	the characterization of a fermented wheat used as a food ingredient and dietary supplement	255:344	The object of this work is the characterization of a fermented wheat used as a food ingredient and dietary supplement.					
30901847	1	35	theme	Fermented	112:120	arg1	cereals					122:128	Fermented cereals	112:128	Fermented cereals	112:128	Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries.					
30901847	1	35	theme	Fermented	112:120	arg1	foods					138:142	staple foods	131:142	staple foods in Asia and Africa	131:161	Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries.					
30901847	6	36	theme	PTP1B	878:882	arg1	enzyme					884:889	PTP1B enzyme	878:889	PTP1B enzyme	878:889	The inhibitory power of the extracts of several batches were investigated on PTP1B enzyme.					
30901847	0	37	from	Activity	52:59	arg1	Enzyme					70:75	PTP1B Enzyme	64:75	PTP1B Enzyme	64:75	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	3	38	theme	prebiotic	501:509	arg1	properties					511:520	the in vitro prebiotic properties	488:520	the in vitro prebiotic properties on Lactobacillus reuteri	488:545	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	39	from	properties	511:520	arg1	PTP1B					435:439	PTP1B	435:439	PTP1B	435:439	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	39	from	properties	511:520	arg1	1B					431:432	protein tyrosine phosphatase 1B	402:432	protein tyrosine phosphatase 1B (PTP1B)	402:440	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	39	from	properties	511:520	arg1	reuteri					539:545	Lactobacillus reuteri	525:545	Lactobacillus reuteri	525:545	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	39	from	properties	511:520	arg1	enzyme					446:451	an enzyme	443:451	an enzyme overexpressed in type-II diabetes	443:485	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	0	40	theme	Whole	21:25	arg1	Wheat					27:31	a Fermented Whole Wheat	9:31	a Fermented Whole Wheat	9:31	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	9	41	theme	reuteri	1245:1251	arg1	grown					1233:1237	grown	1233:1237	grown	1233:1237	The fermented wheat was a suitable substrate for the grown of L. reuteri, recognized for its health properties in the human gut.					
30901847	7	42	theme	first	959:963	arg1	time					965:968	the first time	955:968	the first time	955:968	The product was not able to inhibit the enzyme, otherwise, for the first time, a complete inhibition was observed for schaftoside, a major C-flavonoid of wheat.					
30901847	7	43	theme	wheat	1046:1050	arg1	C-flavonoid					1031:1041	a major C-flavonoid	1023:1041	a major C-flavonoid of wheat	1023:1050	The product was not able to inhibit the enzyme, otherwise, for the first time, a complete inhibition was observed for schaftoside, a major C-flavonoid of wheat.					
30901847	7	43	theme	wheat	1046:1050	arg1	schaftoside					1010:1020	schaftoside	1010:1020	schaftoside	1010:1020	The product was not able to inhibit the enzyme, otherwise, for the first time, a complete inhibition was observed for schaftoside, a major C-flavonoid of wheat.					
30901847	5	44	theme	first	780:784	arg1	time					786:789	the first time	776:789	the first time in wheat	776:798	Methyl ferulate and neocarlinoside were identified for the first time in wheat.					
30901847	0	45	theme	Phenolic	34:41	arg1	Content					43:49	Phenolic Content	34:49	Phenolic Content	34:49	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	9	46	from	properties	1280:1289	arg1	gut					1304:1306	the human gut	1294:1306	the human gut	1294:1306	The fermented wheat was a suitable substrate for the grown of L. reuteri, recognized for its health properties in the human gut.					
30901847	0	47	from	Content	43:49	arg1	Enzyme					70:75	PTP1B Enzyme	64:75	PTP1B Enzyme	64:75	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	3	48	theme	in	492:493	arg1	properties					511:520	the in vitro prebiotic properties	488:520	the in vitro prebiotic properties on Lactobacillus reuteri	488:545	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	2	49	theme	wheat	291:295	arg1	object					232:237	The object	228:237	The object of this work	228:250	The object of this work is the characterization of a fermented wheat used as a food ingredient and dietary supplement.					
30901847	2	49	theme	wheat	291:295	arg1	characterization					259:274	the characterization	255:274	the characterization of a fermented wheat used as a food ingredient and dietary supplement	255:344	The object of this work is the characterization of a fermented wheat used as a food ingredient and dietary supplement.					
30901847	5	50	theme	Methyl	721:726	arg1	ferulate					728:735	Methyl ferulate	721:735	Methyl ferulate	721:735	Methyl ferulate and neocarlinoside were identified for the first time in wheat.					
30901847	1	51	from	interest	197:204	arg1	countries					217:225	Western countries	209:225	Western countries	209:225	Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries.					
30901847	3	52	theme	microbial	555:563	arg1	composition					565:575	the microbial composition	551:575	the microbial composition	551:575	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	53	from	activity	390:397	arg1	PTP1B					435:439	PTP1B	435:439	PTP1B	435:439	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	53	from	activity	390:397	arg1	1B					431:432	protein tyrosine phosphatase 1B	402:432	protein tyrosine phosphatase 1B (PTP1B)	402:440	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	53	from	activity	390:397	arg1	reuteri					539:545	Lactobacillus reuteri	525:545	Lactobacillus reuteri	525:545	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	3	53	from	activity	390:397	arg1	enzyme					446:451	an enzyme	443:451	an enzyme overexpressed in type-II diabetes	443:485	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	2	54	theme	fermented	281:289	arg1	wheat					291:295	a fermented wheat	279:295	a fermented wheat used as a food ingredient and dietary supplement	279:344	The object of this work is the characterization of a fermented wheat used as a food ingredient and dietary supplement.					
30901847	6	55	theme	batches	849:855	arg1	extracts					829:836	the extracts	825:836	the extracts of several batches	825:855	The inhibitory power of the extracts of several batches were investigated on PTP1B enzyme.					
30901847	10	56	theme	basic	1385:1389	arg1	hydrolysis					1391:1400	strong basic hydrolysis	1378:1400	strong basic hydrolysis	1378:1400	The proposed method for phenols is easier compared to those based on strong basic hydrolysis; our results assessed the bound phenols as the major fraction, differently from that suggested by the literature for fermented cereals.					
30901847	3	57	theme	protein	402:408	arg1	phosphatase					419:429	protein tyrosine phosphatase	402:429	protein tyrosine phosphatase 1B (PTP1B)	402:440	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	5	58	from	time	786:789	arg1	wheat					794:798	wheat	794:798	wheat	794:798	Methyl ferulate and neocarlinoside were identified for the first time in wheat.					
30901847	10	59	theme	strong	1378:1383	arg1	hydrolysis					1391:1400	strong basic hydrolysis	1378:1400	strong basic hydrolysis	1378:1400	The proposed method for phenols is easier compared to those based on strong basic hydrolysis; our results assessed the bound phenols as the major fraction, differently from that suggested by the literature for fermented cereals.					
30901847	9	60	theme	human	1298:1302	arg1	gut					1304:1306	the human gut	1294:1306	the human gut	1294:1306	The fermented wheat was a suitable substrate for the grown of L. reuteri, recognized for its health properties in the human gut.					
30901847	4	61	theme	best	708:711	arg1	yields					713:718	best yields	708:718	best yields	708:718	Basic and acidic hydrolysis were tested for an exhaustive recovery of bound phenols: the acidic hydrolysis gave best yields.					
30901847	3	62	theme	tyrosine	410:417	arg1	phosphatase					419:429	protein tyrosine phosphatase	402:429	protein tyrosine phosphatase 1B (PTP1B)	402:440	To this aim, the phenolic composition, the activity on protein tyrosine phosphatase 1B (PTP1B), an enzyme overexpressed in type-II diabetes, the in vitro prebiotic properties on Lactobacillus reuteri and the microbial composition were investigated.					
30901847	0	63	from	Properties	100:109	arg1	Enzyme					70:75	PTP1B Enzyme	64:75	PTP1B Enzyme	64:75	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	2	64	theme	food	307:310	arg1	ingredient					312:321	a food ingredient	305:321	a food ingredient	305:321	The object of this work is the characterization of a fermented wheat used as a food ingredient and dietary supplement.					
30901847	1	65	theme	growing	189:195	arg1	interest					197:204	a growing interest	187:204	a growing interest in Western countries	187:225	Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries.					
30901847	6	66	theme	several	841:847	arg1	batches					849:855	several batches	841:855	several batches	841:855	The inhibitory power of the extracts of several batches were investigated on PTP1B enzyme.					
30901847	0	67	dep	Study	0:4	arg1	Properties					100:109	In Vitro Prebiotic Properties	81:109	In Vitro Prebiotic Properties	81:109	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	0	67	dep	Study	0:4	arg1	Activity					52:59	Activity	52:59	Activity on PTP1B Enzyme	52:75	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	0	67	dep	Study	0:4	arg1	Content					43:49	Phenolic Content	34:49	Phenolic Content	34:49	Study on a Fermented Whole Wheat: Phenolic Content, Activity on PTP1B Enzyme and In Vitro Prebiotic Properties.					
30901847	1	68	from	foods	138:142	arg1	Asia					147:150	Asia	147:150	Asia	147:150	Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries.					
30901847	1	68	from	foods	138:142	arg1	Africa					156:161	Africa	156:161	Africa	156:161	Fermented cereals, staple foods in Asia and Africa, are recently receiving a growing interest in Western countries.					
30901847	4	69	theme	acidic	606:611	arg1	hydrolysis					613:622	Basic and acidic hydrolysis	596:622	Basic and acidic hydrolysis	596:622	Basic and acidic hydrolysis were tested for an exhaustive recovery of bound phenols: the acidic hydrolysis gave best yields.					
30362476	3	0	theme	effective	965:973	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	0	theme	effective	965:973	arg1	activity					986:993	effective anti-tumor activity	965:993	effective anti-tumor activity	965:993	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	4	1	theme	unique	1042:1047	arg1	nanostructure					1061:1073	its unique amphiphilic nanostructure	1038:1073	its unique amphiphilic nanostructure	1038:1073	Benefiting from its unique amphiphilic nanostructure, PRMO@DOX can form water-soluble prodrug nanoparticles in aqueous media.					
30362476	5	2	from	bond	1174:1177	arg1	prodrug					1186:1192	the prodrug	1182:1192	the prodrug	1182:1192	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	2	3	theme	methacrylate	622:633	arg1	prodrug					550:556	a α-CD-based PR polymeric prodrug	524:556	a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	524:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	6	4	theme	nanomedicine	1555:1566	arg1	development					1521:1531	the development	1517:1531	the development of polyrotaxane-based nanomedicine for enhanced cancer therapy	1517:1594	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	3	5	theme	low	999:1001	arg1	toxicity					1012:1019	low systemic toxicity	999:1019	low systemic toxicity	999:1019	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	5	theme	low	999:1001	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	6	6	theme	antitumor	1434:1442	arg1	efficacy					1444:1451	a remarkable antitumor efficacy	1421:1451	a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy	1421:1594	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	1	7	theme	CD	197:198	arg1	PRs					216:218	PRs	216:218	PRs	216:218	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	1	7	theme	CD	197:198	arg1	polyrotaxanes					201:213	cyclodextrin (CD) polyrotaxanes	183:213	cyclodextrin (CD) polyrotaxanes (PRs)	183:219	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	2	8	dep	methacrylate	622:633	arg1	PR-PDOX-co-POEGMA					637:653	PR-PDOX-co-POEGMA	637:653	PR-PDOX-co-POEGMA	637:653	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	4	9	theme	prodrug	1108:1114	arg1	nanoparticles					1116:1128	water-soluble prodrug nanoparticles	1094:1128	water-soluble prodrug nanoparticles	1094:1128	Benefiting from its unique amphiphilic nanostructure, PRMO@DOX can form water-soluble prodrug nanoparticles in aqueous media.					
30362476	1	10	theme	good	418:421	arg1	biocompatibility					423:438	good biocompatibility	418:438	good biocompatibility	418:438	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	2	11	theme	methyl	609:614	arg1	methacrylate					622:633	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate]	561:634	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	561:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	2	12	theme	glycol	601:606	arg1	methacrylate					622:633	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate]	561:634	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	561:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	1	13	theme	supramolecular	147:160	arg1	polymer					174:180	a supramolecular macrocyclic polymer	145:180	a supramolecular macrocyclic polymer	145:180	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	1	13	theme	supramolecular	147:160	arg1	advantages					231:240	many advantages	226:240	many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility	226:438	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	3	14	theme	desirable	854:862	arg1	release					956:962	acid-active controlled release	933:962	acid-active controlled release	933:962	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	14	theme	desirable	854:862	arg1	toxicity					1012:1019	low systemic toxicity	999:1019	low systemic toxicity	999:1019	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	14	theme	desirable	854:862	arg1	activity					986:993	effective anti-tumor activity	965:993	effective anti-tumor activity	965:993	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	14	theme	desirable	854:862	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	14	theme	desirable	854:862	arg1	uptake					925:930	fast cellular uptake	911:930	fast cellular uptake	911:930	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	2	15	theme	polymeric	540:548	arg1	prodrug					550:556	a α-CD-based PR polymeric prodrug	524:556	a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	524:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	5	16	theme	tumor	1285:1289	arg1	damaging					1309:1316	damaging	1309:1316	damaging the nuclei and mitochondria of tumor cells	1309:1359	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	5	16	theme	tumor	1285:1289	arg1	microenvironment					1291:1306	an acidic tumor microenvironment	1275:1306	an acidic tumor microenvironment	1275:1306	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	6	17	theme	enhanced	1572:1579	arg1	therapy					1588:1594	enhanced cancer therapy	1572:1594	enhanced cancer therapy	1572:1594	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	3	18	theme	rates	894:898	arg1	release					956:962	acid-active controlled release	933:962	acid-active controlled release	933:962	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	18	theme	rates	894:898	arg1	toxicity					1012:1019	low systemic toxicity	999:1019	low systemic toxicity	999:1019	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	18	theme	rates	894:898	arg1	activity					986:993	effective anti-tumor activity	965:993	effective anti-tumor activity	965:993	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	18	theme	rates	894:898	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	18	theme	rates	894:898	arg1	uptake					925:930	fast cellular uptake	911:930	fast cellular uptake	911:930	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	2	19	theme	α-CD-based	526:535	arg1	prodrug					550:556	a α-CD-based PR polymeric prodrug	524:556	a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	524:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	6	20	theme	in	1367:1368	arg1	experiments					1388:1398	Both in vitro and in vivo experiments	1362:1398	Both in vitro and in vivo experiments	1362:1398	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	1	21	theme	cyclodextrin	183:194	arg1	PRs					216:218	PRs	216:218	PRs	216:218	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	1	21	theme	cyclodextrin	183:194	arg1	polyrotaxanes					201:213	cyclodextrin (CD) polyrotaxanes	183:213	cyclodextrin (CD) polyrotaxanes (PRs)	183:219	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	3	22	theme	drug	881:884	arg1	%					907:907	>25 wt%	901:907	>25 wt%	901:907	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	22	theme	drug	881:884	arg1	rates					894:898	high drug loading rates	876:898	high drug loading rates (>25 wt%)	876:908	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	23	theme	PRMO	838:841	arg1	DOX					843:845	The obtained PRMO@DOX	825:845	The obtained PRMO@DOX	825:845	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	4	24	theme	aqueous	1133:1139	arg1	media					1141:1145	aqueous media	1133:1145	aqueous media	1133:1145	Benefiting from its unique amphiphilic nanostructure, PRMO@DOX can form water-soluble prodrug nanoparticles in aqueous media.					
30362476	2	25	theme	PRMO	667:670	arg1	DOX					672:674	PRMO@DOX	667:674	PRMO@DOX	667:674	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	0	26	theme	tumor	129:133	arg1	cells					135:139	tumor cells	129:139	tumor cells	129:139	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin polyrotaxanes damaging both mitochondria and nuclei of tumor cells.					
30362476	1	27	theme	adjustable	385:394	arg1	size					409:412	adjustable nanoparticle size	385:412	adjustable nanoparticle size	385:412	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	6	28	dep	in	1367:1368	arg1	vitro					1370:1374	vitro	1370:1374	vitro	1370:1374	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	2	29	theme	drug	687:690	arg1	leakage					692:698	drug leakage	687:698	drug leakage	687:698	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	0	30	theme	Acid-active	0:10	arg1	nanoparticles					38:50	Acid-active supramolecular anticancer nanoparticles	0:50	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin	0:72	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin polyrotaxanes damaging both mitochondria and nuclei of tumor cells.					
30362476	1	31	theme	stable	280:285	arg1	composition					296:306	stable chemical composition	280:306	stable chemical composition	280:306	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	3	32	theme	cellular	916:923	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	32	theme	cellular	916:923	arg1	uptake					925:930	fast cellular uptake	911:930	fast cellular uptake	911:930	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	0	33	theme	anticancer	27:36	arg1	nanoparticles					38:50	Acid-active supramolecular anticancer nanoparticles	0:50	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin	0:72	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin polyrotaxanes damaging both mitochondria and nuclei of tumor cells.					
30362476	0	34	dep	damaging	88:95	arg1	both					97:100	both	97:100	both	97:100	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin polyrotaxanes damaging both mitochondria and nuclei of tumor cells.					
30362476	1	35	theme	abundant	309:316	arg1	groups					342:347	abundant functionalized hydroxyl groups	309:347	abundant functionalized hydroxyl groups	309:347	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	3	36	theme	controlled	945:954	arg1	release					956:962	acid-active controlled release	933:962	acid-active controlled release	933:962	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	36	theme	controlled	945:954	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	2	37	theme	therapeutic	487:497	arg1	nanoparticles					499:511	acid-active therapeutic nanoparticles	475:511	acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	475:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	1	38	theme	hydroxyl	333:340	arg1	groups					342:347	abundant functionalized hydroxyl groups	309:347	abundant functionalized hydroxyl groups	309:347	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	5	39	dep	nuclei	1322:1327	arg1	the					1318:1320	the	1318:1320	the	1318:1320	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	4	40	theme	@	1080:1080	arg1	DOX					1081:1083	PRMO@DOX	1076:1083	PRMO@DOX	1076:1083	Benefiting from its unique amphiphilic nanostructure, PRMO@DOX can form water-soluble prodrug nanoparticles in aqueous media.					
30362476	1	41	theme	many	226:229	arg1	polymer					174:180	a supramolecular macrocyclic polymer	145:180	a supramolecular macrocyclic polymer	145:180	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	1	41	theme	many	226:229	arg1	advantages					231:240	many advantages	226:240	many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility	226:438	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	3	42	theme	anti-tumor	975:984	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	42	theme	anti-tumor	975:984	arg1	activity					986:993	effective anti-tumor activity	965:993	effective anti-tumor activity	965:993	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	5	43	theme	cells	1355:1359	arg1	mitochondria					1333:1344	mitochondria	1333:1344	mitochondria	1333:1344	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	5	43	theme	cells	1355:1359	arg1	nuclei					1322:1327	nuclei	1322:1327	nuclei	1322:1327	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	5	44	dep	damaging	1309:1316	arg1	mitochondria					1333:1344	mitochondria	1333:1344	mitochondria	1333:1344	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	5	44	dep	damaging	1309:1316	arg1	nuclei					1322:1327	nuclei	1322:1327	nuclei	1322:1327	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	4	45	theme	amphiphilic	1049:1059	arg1	nanostructure					1061:1073	its unique amphiphilic nanostructure	1038:1073	its unique amphiphilic nanostructure	1038:1073	Benefiting from its unique amphiphilic nanostructure, PRMO@DOX can form water-soluble prodrug nanoparticles in aqueous media.					
30362476	5	46	theme	acid-active	1152:1162	arg1	bond					1174:1177	The acid-active hydrazone bond	1148:1177	The acid-active hydrazone bond in the prodrug	1148:1192	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	6	47	theme	therapeutic	1461:1471	arg1	platform					1473:1480	this therapeutic platform	1456:1480	this therapeutic platform	1456:1480	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	3	48	theme	systemic	1003:1010	arg1	toxicity					1012:1019	low systemic toxicity	999:1019	low systemic toxicity	999:1019	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	48	theme	systemic	1003:1010	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	2	49	theme	ether	616:620	arg1	methacrylate					622:633	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate]	561:634	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	561:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	2	50	theme	maximal	766:772	arg1	efficacy					784:791	maximal treatment efficacy	766:791	maximal treatment efficacy of supramolecular therapeutics	766:822	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	6	51	theme	polyrotaxane-based	1536:1553	arg1	nanomedicine					1555:1566	polyrotaxane-based nanomedicine	1536:1566	polyrotaxane-based nanomedicine for enhanced cancer therapy	1536:1594	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	5	52	theme	hydrazone	1164:1172	arg1	bond					1174:1177	The acid-active hydrazone bond	1148:1177	The acid-active hydrazone bond in the prodrug	1148:1192	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	2	53	theme	supramolecular	796:809	arg1	therapeutics					811:822	supramolecular therapeutics	796:822	supramolecular therapeutics	796:822	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	2	54	theme	-co-poly[	582:590	arg1	methacrylate					622:633	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate]	561:634	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	561:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	4	55	theme	water-soluble	1094:1106	arg1	nanoparticles					1116:1128	water-soluble prodrug nanoparticles	1094:1128	water-soluble prodrug nanoparticles	1094:1128	Benefiting from its unique amphiphilic nanostructure, PRMO@DOX can form water-soluble prodrug nanoparticles in aqueous media.					
30362476	6	56	theme	new	1500:1502	arg1	strategy					1504:1511	a new strategy	1498:1511	a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy	1498:1594	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	2	57	theme	doxorubicin	570:580	arg1	methacrylate					622:633	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate]	561:634	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	561:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	3	58	theme	high	876:879	arg1	%					907:907	>25 wt%	901:907	>25 wt%	901:907	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	58	theme	high	876:879	arg1	rates					894:898	high drug loading rates	876:898	high drug loading rates (>25 wt%)	876:908	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	2	59	theme	ethylene	592:599	arg1	methacrylate					622:633	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate]	561:634	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	561:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	5	60	dep	break	1198:1202	arg1	release					1213:1219	release	1213:1219	release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells	1213:1359	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	5	61	theme	acidic	1278:1283	arg1	damaging					1309:1316	damaging	1309:1316	damaging the nuclei and mitochondria of tumor cells	1309:1359	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	5	61	theme	acidic	1278:1283	arg1	microenvironment					1291:1306	an acidic tumor microenvironment	1275:1306	an acidic tumor microenvironment	1275:1306	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	3	62	theme	obtained	829:836	arg1	DOX					843:845	The obtained PRMO@DOX	825:845	The obtained PRMO@DOX	825:845	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	6	63	theme	in	1380:1381	arg1	experiments					1388:1398	Both in vitro and in vivo experiments	1362:1398	Both in vitro and in vivo experiments	1362:1398	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	6	64	theme	cancer	1581:1586	arg1	therapy					1588:1594	enhanced cancer therapy	1572:1594	enhanced cancer therapy	1572:1594	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	1	65	theme	macrocyclic	162:172	arg1	polymer					174:180	a supramolecular macrocyclic polymer	145:180	a supramolecular macrocyclic polymer	145:180	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	1	65	theme	macrocyclic	162:172	arg1	advantages					231:240	many advantages	226:240	many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility	226:438	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	2	66	theme	PR	537:538	arg1	prodrug					550:556	a α-CD-based PR polymeric prodrug	524:556	a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	524:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	2	67	theme	tumor	735:739	arg1	cells					741:745	tumor cells	735:745	tumor cells	735:745	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	5	68	theme	drug	1221:1224	arg1	molecules					1226:1234	drug molecules	1221:1234	drug molecules precisely and in a timely manner	1221:1267	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	5	69	theme	timely	1255:1260	arg1	manner					1262:1267	a timely manner	1253:1267	a timely manner	1253:1267	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	1	70	theme	biological	364:373	arg1	barriers					375:382	biological barriers	364:382	biological barriers	364:382	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	3	71	theme	loading	886:892	arg1	%					907:907	>25 wt%	901:907	>25 wt%	901:907	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	71	theme	loading	886:892	arg1	rates					894:898	high drug loading rates	876:898	high drug loading rates (>25 wt%)	876:908	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	2	72	theme	PRs-poly	561:568	arg1	methacrylate					622:633	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate]	561:634	PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	561:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	2	73	theme	@	671:671	arg1	DOX					672:674	PRMO@DOX	667:674	PRMO@DOX	667:674	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	3	74	theme	@	842:842	arg1	DOX					843:845	The obtained PRMO@DOX	825:845	The obtained PRMO@DOX	825:845	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	0	75	theme	cells	135:139	arg1	mitochondria					102:113	mitochondria	102:113	mitochondria	102:113	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin polyrotaxanes damaging both mitochondria and nuclei of tumor cells.					
30362476	0	75	theme	cells	135:139	arg1	nuclei					119:124	nuclei	119:124	nuclei	119:124	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin polyrotaxanes damaging both mitochondria and nuclei of tumor cells.					
30362476	0	76	theme	supramolecular	12:25	arg1	nanoparticles					38:50	Acid-active supramolecular anticancer nanoparticles	0:50	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin	0:72	Acid-active supramolecular anticancer nanoparticles based on cyclodextrin polyrotaxanes damaging both mitochondria and nuclei of tumor cells.					
30362476	1	77	theme	nanoparticle	396:407	arg1	size					409:412	adjustable nanoparticle size	385:412	adjustable nanoparticle size	385:412	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	6	78	dep	in	1380:1381	arg1	vivo					1383:1386	vivo	1383:1386	vivo	1383:1386	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	3	79	theme	wt	905:906	arg1	%					907:907	>25 wt%	901:907	>25 wt%	901:907	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	79	theme	wt	905:906	arg1	rates					894:898	high drug loading rates	876:898	high drug loading rates (>25 wt%)	876:908	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	6	80	theme	remarkable	1423:1432	arg1	efficacy					1444:1451	a remarkable antitumor efficacy	1421:1451	a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy	1421:1594	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30362476	1	81	theme	chemical	287:294	arg1	composition					296:306	stable chemical composition	280:306	stable chemical composition	280:306	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	2	82	theme	treatment	774:782	arg1	efficacy					784:791	maximal treatment efficacy	766:791	maximal treatment efficacy of supramolecular therapeutics	766:822	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	3	83	theme	fast	911:914	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	83	theme	fast	911:914	arg1	uptake					925:930	fast cellular uptake	911:930	fast cellular uptake	911:930	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	2	84	theme	nanoparticles	499:511	arg1	class					466:470	a class	464:470	a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells	464:745	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	1	85	theme	functionalized	318:331	arg1	groups					342:347	abundant functionalized hydroxyl groups	309:347	abundant functionalized hydroxyl groups	309:347	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	2	86	theme	acid-active	475:485	arg1	nanoparticles					499:511	acid-active therapeutic nanoparticles	475:511	acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX)	475:675	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	1	87	contain	have	221:224	arg1	PRs					216:218	PRs	216:218	PRs	216:218	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	1	87	contain	have	221:224	arg2	polymer					174:180	a supramolecular macrocyclic polymer	145:180	a supramolecular macrocyclic polymer	145:180	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	1	87	contain	have	221:224	arg1	polyrotaxanes					201:213	cyclodextrin (CD) polyrotaxanes	183:213	cyclodextrin (CD) polyrotaxanes (PRs)	183:219	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	1	87	contain	have	221:224	arg2	advantages					231:240	many advantages	226:240	many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility	226:438	As a supramolecular macrocyclic polymer, cyclodextrin (CD) polyrotaxanes (PRs) have many advantages for developing nanomedicines, such as stable chemical composition, abundant functionalized hydroxyl groups, moving across biological barriers, adjustable nanoparticle size and good biocompatibility.					
30362476	3	88	theme	acid-active	933:943	arg1	release					956:962	acid-active controlled release	933:962	acid-active controlled release	933:962	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	3	88	theme	acid-active	933:943	arg1	features					864:871	desirable features	854:871	desirable features	854:871	The obtained PRMO@DOX showed desirable features of high drug loading rates (>25 wt%), fast cellular uptake, acid-active controlled release, effective anti-tumor activity and low systemic toxicity.					
30362476	4	89	theme	PRMO	1076:1079	arg1	DOX					1081:1083	PRMO@DOX	1076:1083	PRMO@DOX	1076:1083	Benefiting from its unique amphiphilic nanostructure, PRMO@DOX can form water-soluble prodrug nanoparticles in aqueous media.					
30362476	5	90	theme	tumor	1349:1353	arg1	cells					1355:1359	tumor cells	1349:1359	tumor cells	1349:1359	The acid-active hydrazone bond in the prodrug can break and thus release drug molecules precisely and in a timely manner under an acidic tumor microenvironment, damaging the nuclei and mitochondria of tumor cells.					
30362476	2	91	theme	therapeutics	811:822	arg1	efficacy					784:791	maximal treatment efficacy	766:791	maximal treatment efficacy of supramolecular therapeutics	766:822	Herein, we synthesized a class of acid-active therapeutic nanoparticles comprising a α-CD-based PR polymeric prodrug of PRs-poly(doxorubicin)-co-poly[(ethylene glycol) methyl ether methacrylate] (PR-PDOX-co-POEGMA, denoted as PRMO@DOX) to reduce drug leakage and selectively deliver drugs into tumor cells, aiming to achieve maximal treatment efficacy of supramolecular therapeutics.					
30362476	6	92	theme	platform	1473:1480	arg1	efficacy					1444:1451	a remarkable antitumor efficacy	1421:1451	a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy	1421:1594	Both in vitro and in vivo experiments clearly demonstrated a remarkable antitumor efficacy of this therapeutic platform, which provided a new strategy for the development of polyrotaxane-based nanomedicine for enhanced cancer therapy.					
30978372	2	0	theme	soluble	443:449	arg1	solids					451:456	total soluble solids	437:456	total soluble solids	437:456	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	5	1	theme	Antioxidant	743:753	arg1	evaluation					755:764	Antioxidant evaluation	743:764	Antioxidant evaluation	743:764	Antioxidant evaluation showed that extracts from finger lime cultivar of 'XiangBin' exhibited better antioxidant capacities than cultivar of 'LiSiKe', especially in peel.					
30978372	9	2	from	knowledge	1341:1349	arg1	characteristics					1373:1387	physiochemical characteristics	1358:1387	physiochemical characteristics	1358:1387	This study may expand our knowledge on the physiochemical characteristics and bioactive properties of citrus fruits.					
30978372	9	2	from	knowledge	1341:1349	arg1	properties					1403:1412	bioactive properties	1393:1412	bioactive properties	1393:1412	This study may expand our knowledge on the physiochemical characteristics and bioactive properties of citrus fruits.					
30978372	2	3	from	features	396:403	arg1	contents					497:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	4	4	theme	phenolics	665:673	arg1	composition					675:685	The phenolics composition	661:685	The phenolics composition of finger limes	661:701	The phenolics composition of finger limes showed cultivar and tissue specificity.					
30978372	6	5	theme	BV-2 cell	982:990	arg1	line					992:995	the mouse microglia BV-2 cell line	962:995	the mouse microglia BV-2 cell line	962:995	LPS-induced NO-releasing model was performed in the mouse microglia BV-2 cell line.					
30978372	0	6	theme	finger	67:72	arg1	species					58:64	a unique citrus species	42:64	a unique citrus species	42:64	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	6	theme	finger	67:72	arg1	lime					74:77	finger lime	67:77	finger lime (Citrus australasica)	67:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	6	theme	finger	67:72	arg1	australasica					87:98	Citrus australasica	80:98	Citrus australasica	80:98	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	7	7	theme	TNFα	1128:1131	arg1	elevation					1133:1141	TNFα elevation	1128:1141	TNFα elevation	1128:1141	Results illustrated that finger limes inhibited the NO-releasing and the inflammation-related cytokines including IL-1β, IL-6 and TNFα elevation.					
30978372	5	8	theme	LiSiKe	885:890	arg1	cultivar					872:879	cultivar	872:879	cultivar of 'LiSiKe'	872:891	Antioxidant evaluation showed that extracts from finger lime cultivar of 'XiangBin' exhibited better antioxidant capacities than cultivar of 'LiSiKe', especially in peel.					
30978372	2	9	theme	water-soluble	459:471	arg1	pectin					473:478	water-soluble pectin	459:478	water-soluble pectin	459:478	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	0	10	theme	Citrus	80:85	arg1	lime					74:77	finger lime	67:77	finger lime (Citrus australasica)	67:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	10	theme	Citrus	80:85	arg1	australasica					87:98	Citrus australasica	80:98	Citrus australasica	80:98	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	11	from	Identification	0:13	arg1	species					58:64	a unique citrus species	42:64	a unique citrus species	42:64	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	11	from	Identification	0:13	arg1	lime					74:77	finger lime	67:77	finger lime (Citrus australasica)	67:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	11	from	Identification	0:13	arg1	line					163:166	BV-2 cell line	153:166	BV-2 cell line	153:166	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	12	from	NO-releasing	137:148	arg1	line					163:166	BV-2 cell line	153:166	BV-2 cell line	153:166	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	5	13	from	cultivar	804:811	arg1	extracts					778:785	extracts	778:785	extracts from finger lime cultivar of 'XiangBin'	778:825	Antioxidant evaluation showed that extracts from finger lime cultivar of 'XiangBin' exhibited better antioxidant capacities than cultivar of 'LiSiKe', especially in peel.					
30978372	8	14	theme	IκBα	1309:1312	arg1	upregulation					1210:1221	LPS-induced upregulation	1198:1221	LPS-induced upregulation of iNOS	1198:1229	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	14	theme	IκBα	1309:1312	arg1	downregulation					1291:1304	LPS-induced downregulation	1279:1304	LPS-induced downregulation of IκBα	1279:1312	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	14	theme	IκBα	1309:1312	arg1	JAK2					1238:1241	JAK2	1238:1241	JAK2	1238:1241	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	14	theme	IκBα	1309:1312	arg1	TNFα					1244:1247	TNFα	1244:1247	TNFα	1244:1247	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	14	theme	IκBα	1309:1312	arg1	IL-1β					1262:1266	IL-1β	1262:1266	IL-1β	1262:1266	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	14	theme	IκBα	1309:1312	arg1	NF-κB					1269:1273	NF-κB	1269:1273	NF-κB	1269:1273	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	14	theme	IκBα	1309:1312	arg1	IL-6					1232:1235	IL-6	1232:1235	IL-6	1232:1235	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	14	theme	IκBα	1309:1312	arg1	TLR2					1250:1253	TLR2	1250:1253	TLR2	1250:1253	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	14	theme	IκBα	1309:1312	arg1	TLR4					1256:1259	TLR4	1256:1259	TLR4	1256:1259	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	9	15	theme	citrus	1417:1422	arg1	fruits					1424:1429	citrus fruits	1417:1429	citrus fruits	1417:1429	This study may expand our knowledge on the physiochemical characteristics and bioactive properties of citrus fruits.					
30978372	2	16	theme	pectin	473:478	arg1	contents					497:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	5	17	theme	better	837:842	arg1	capacities					856:865	better antioxidant capacities	837:865	better antioxidant capacities	837:865	Antioxidant evaluation showed that extracts from finger lime cultivar of 'XiangBin' exhibited better antioxidant capacities than cultivar of 'LiSiKe', especially in peel.					
30978372	2	18	theme	weight	421:426	arg1	contents					497:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	2	19	theme	total	437:441	arg1	solids					451:456	total soluble solids	437:456	total soluble solids	437:456	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	9	20	theme	bioactive	1393:1401	arg1	properties					1403:1412	bioactive properties	1393:1412	bioactive properties	1393:1412	This study may expand our knowledge on the physiochemical characteristics and bioactive properties of citrus fruits.					
30978372	5	21	theme	lime	799:802	arg1	cultivar					804:811	finger lime cultivar	792:811	finger lime cultivar of 'XiangBin'	792:825	Antioxidant evaluation showed that extracts from finger lime cultivar of 'XiangBin' exhibited better antioxidant capacities than cultivar of 'LiSiKe', especially in peel.					
30978372	2	22	theme	lime	365:368	arg1	fruits					370:375	Finger lime fruits	358:375	Finger lime fruits	358:375	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	5	23	theme	finger	792:797	arg1	cultivar					804:811	finger lime cultivar	792:811	finger lime cultivar of 'XiangBin'	792:825	Antioxidant evaluation showed that extracts from finger lime cultivar of 'XiangBin' exhibited better antioxidant capacities than cultivar of 'LiSiKe', especially in peel.					
30978372	1	24	theme	fruit	252:256	arg1	characteristics					258:272	its fruit characteristics	248:272	its fruit characteristics	248:272	In this study, a unique citrus species (Citrus australasica) was selected, and its fruit characteristics, phenolic compounds and ability to inhibit inflammation were preliminarily studied.					
30978372	7	25	theme	inflammation-related	1071:1090	arg1	IL-6					1119:1122	IL-6	1119:1122	IL-6	1119:1122	Results illustrated that finger limes inhibited the NO-releasing and the inflammation-related cytokines including IL-1β, IL-6 and TNFα elevation.					
30978372	7	25	theme	inflammation-related	1071:1090	arg1	elevation					1133:1141	TNFα elevation	1128:1141	TNFα elevation	1128:1141	Results illustrated that finger limes inhibited the NO-releasing and the inflammation-related cytokines including IL-1β, IL-6 and TNFα elevation.					
30978372	7	25	theme	inflammation-related	1071:1090	arg1	IL-1β					1112:1116	IL-1β	1112:1116	IL-1β	1112:1116	Results illustrated that finger limes inhibited the NO-releasing and the inflammation-related cytokines including IL-1β, IL-6 and TNFα elevation.					
30978372	7	25	theme	inflammation-related	1071:1090	arg1	cytokines					1092:1100	the inflammation-related cytokines	1067:1100	the inflammation-related cytokines including IL-1β, IL-6 and TNFα elevation	1067:1141	Results illustrated that finger limes inhibited the NO-releasing and the inflammation-related cytokines including IL-1β, IL-6 and TNFα elevation.					
30978372	2	26	theme	Finger	358:363	arg1	fruits					370:375	Finger lime fruits	358:375	Finger lime fruits	358:375	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	2	27	theme	size	415:418	arg1	contents					497:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	3	28	theme	UPLC-DAD	531:538	arg1	analysis					540:547	UPLC-DAD analysis	531:547	UPLC-DAD analysis	531:547	Combining UPLC-HRMS and UPLC-DAD analysis, 31 phenolics, 1 secoiridoid derivative and 1 neolignan glycoside were preliminarily identified and quantified.					
30978372	0	29	theme	compounds	27:35	arg1	inhibition					111:120	their inhibition	105:120	their inhibition of LPS-induced NO-releasing in BV-2 cell line	105:166	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	29	theme	compounds	27:35	arg1	Identification					0:13	Identification	0:13	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica)	0:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	8	30	theme	lime	1173:1176	arg1	extracts					1178:1185	finger lime extracts	1166:1185	finger lime extracts	1166:1185	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	9	31	theme	fruits	1424:1429	arg1	characteristics					1373:1387	physiochemical characteristics	1358:1387	physiochemical characteristics	1358:1387	This study may expand our knowledge on the physiochemical characteristics and bioactive properties of citrus fruits.					
30978372	9	31	theme	fruits	1424:1429	arg1	properties					1403:1412	bioactive properties	1393:1412	bioactive properties	1393:1412	This study may expand our knowledge on the physiochemical characteristics and bioactive properties of citrus fruits.					
30978372	4	32	theme	tissue	723:728	arg1	specificity					730:740	tissue specificity	723:740	tissue specificity	723:740	The phenolics composition of finger limes showed cultivar and tissue specificity.					
30978372	2	33	theme	shape	408:412	arg1	contents					497:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	3	34	theme	neolignan	595:603	arg1	phenolics					553:561	31 phenolics	550:561	31 phenolics	550:561	Combining UPLC-HRMS and UPLC-DAD analysis, 31 phenolics, 1 secoiridoid derivative and 1 neolignan glycoside were preliminarily identified and quantified.					
30978372	3	34	theme	neolignan	595:603	arg1	glycoside					605:613	1 secoiridoid derivative and 1 neolignan glycoside	564:613	glycoside	605:613	Combining UPLC-HRMS and UPLC-DAD analysis, 31 phenolics, 1 secoiridoid derivative and 1 neolignan glycoside were preliminarily identified and quantified.					
30978372	8	35	theme	finger	1166:1171	arg1	extracts					1178:1185	finger lime extracts	1166:1185	finger lime extracts	1166:1185	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	5	36	theme	XiangBin	817:824	arg1	cultivar					804:811	finger lime cultivar	792:811	finger lime cultivar of 'XiangBin'	792:825	Antioxidant evaluation showed that extracts from finger lime cultivar of 'XiangBin' exhibited better antioxidant capacities than cultivar of 'LiSiKe', especially in peel.					
30978372	0	37	from	line	163:166	arg1	inhibition					111:120	their inhibition	105:120	their inhibition of LPS-induced NO-releasing in BV-2 cell line	105:166	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	37	from	line	163:166	arg1	NO-releasing					137:148	NO-releasing	137:148	NO-releasing	137:148	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	37	from	line	163:166	arg1	Identification					0:13	Identification	0:13	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica)	0:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	2	38	theme	acids	491:495	arg1	contents					497:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	2	39	theme	distinctive	384:394	arg1	features					396:403	distinctive features	384:403	distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	384:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	2	40	theme	colour	429:434	arg1	contents					497:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	6	41	theme	NO-releasing	926:937	arg1	model					939:943	LPS-induced NO-releasing model	914:943	LPS-induced NO-releasing model	914:943	LPS-induced NO-releasing model was performed in the mouse microglia BV-2 cell line.					
30978372	6	42	theme	microglia	972:980	arg1	line					992:995	the mouse microglia BV-2 cell line	962:995	the mouse microglia BV-2 cell line	962:995	LPS-induced NO-releasing model was performed in the mouse microglia BV-2 cell line.					
30978372	1	43	theme	unique	186:191	arg1	species					200:206	a unique citrus species	184:206	a unique citrus species (Citrus australasica)	184:228	In this study, a unique citrus species (Citrus australasica) was selected, and its fruit characteristics, phenolic compounds and ability to inhibit inflammation were preliminarily studied.					
30978372	1	43	theme	unique	186:191	arg1	australasica					216:227	Citrus australasica	209:227	Citrus australasica	209:227	In this study, a unique citrus species (Citrus australasica) was selected, and its fruit characteristics, phenolic compounds and ability to inhibit inflammation were preliminarily studied.					
30978372	0	44	theme	citrus	51:56	arg1	species					58:64	a unique citrus species	42:64	a unique citrus species	42:64	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	44	theme	citrus	51:56	arg1	lime					74:77	finger lime	67:77	finger lime (Citrus australasica)	67:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	2	45	theme	sugar	481:485	arg1	contents					497:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	0	46	theme	NO-releasing	137:148	arg1	inhibition					111:120	their inhibition	105:120	their inhibition of LPS-induced NO-releasing in BV-2 cell line	105:166	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	46	theme	NO-releasing	137:148	arg1	Identification					0:13	Identification	0:13	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica)	0:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	47	from	species	58:64	arg1	inhibition					111:120	their inhibition	105:120	their inhibition of LPS-induced NO-releasing in BV-2 cell line	105:166	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	47	from	species	58:64	arg1	Identification					0:13	Identification	0:13	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica)	0:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	47	from	species	58:64	arg1	compounds					27:35	phenolic compounds	18:35	phenolic compounds from a unique citrus species, finger lime (Citrus australasica)	18:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	8	48	theme	LPS-induced	1279:1289	arg1	downregulation					1291:1304	LPS-induced downregulation	1279:1304	LPS-induced downregulation of IκBα	1279:1312	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	0	49	theme	unique	44:49	arg1	species					58:64	a unique citrus species	42:64	a unique citrus species	42:64	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	49	theme	unique	44:49	arg1	lime					74:77	finger lime	67:77	finger lime (Citrus australasica)	67:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	1	50	theme	phenolic	275:282	arg1	compounds					284:292	phenolic compounds	275:292	phenolic compounds	275:292	In this study, a unique citrus species (Citrus australasica) was selected, and its fruit characteristics, phenolic compounds and ability to inhibit inflammation were preliminarily studied.					
30978372	9	51	theme	physiochemical	1358:1371	arg1	characteristics					1373:1387	physiochemical characteristics	1358:1387	physiochemical characteristics	1358:1387	This study may expand our knowledge on the physiochemical characteristics and bioactive properties of citrus fruits.					
30978372	6	52	theme	mouse	966:970	arg1	line					992:995	the mouse microglia BV-2 cell line	962:995	the mouse microglia BV-2 cell line	962:995	LPS-induced NO-releasing model was performed in the mouse microglia BV-2 cell line.					
30978372	8	53	theme	iNOS	1226:1229	arg1	upregulation					1210:1221	LPS-induced upregulation	1198:1221	LPS-induced upregulation of iNOS	1198:1229	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	53	theme	iNOS	1226:1229	arg1	downregulation					1291:1304	LPS-induced downregulation	1279:1304	LPS-induced downregulation of IκBα	1279:1312	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	53	theme	iNOS	1226:1229	arg1	JAK2					1238:1241	JAK2	1238:1241	JAK2	1238:1241	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	53	theme	iNOS	1226:1229	arg1	TNFα					1244:1247	TNFα	1244:1247	TNFα	1244:1247	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	53	theme	iNOS	1226:1229	arg1	IL-1β					1262:1266	IL-1β	1262:1266	IL-1β	1262:1266	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	53	theme	iNOS	1226:1229	arg1	NF-κB					1269:1273	NF-κB	1269:1273	NF-κB	1269:1273	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	53	theme	iNOS	1226:1229	arg1	IL-6					1232:1235	IL-6	1232:1235	IL-6	1232:1235	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	53	theme	iNOS	1226:1229	arg1	TLR2					1250:1253	TLR2	1250:1253	TLR2	1250:1253	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	8	53	theme	iNOS	1226:1229	arg1	TLR4					1256:1259	TLR4	1256:1259	TLR4	1256:1259	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	0	54	theme	BV-2 cell	153:161	arg1	line					163:166	BV-2 cell line	153:166	BV-2 cell line	153:166	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	3	55	theme	secoiridoid	566:576	arg1	phenolics					553:561	31 phenolics	550:561	31 phenolics	550:561	Combining UPLC-HRMS and UPLC-DAD analysis, 31 phenolics, 1 secoiridoid derivative and 1 neolignan glycoside were preliminarily identified and quantified.					
30978372	3	55	theme	secoiridoid	566:576	arg1	derivative					578:587	1 secoiridoid derivative and 1 neolignan glycoside	564:613	derivative	578:587	Combining UPLC-HRMS and UPLC-DAD analysis, 31 phenolics, 1 secoiridoid derivative and 1 neolignan glycoside were preliminarily identified and quantified.					
30978372	4	56	theme	limes	697:701	arg1	composition					675:685	The phenolics composition	661:685	The phenolics composition of finger limes	661:701	The phenolics composition of finger limes showed cultivar and tissue specificity.					
30978372	1	57	theme	citrus	193:198	arg1	species					200:206	a unique citrus species	184:206	a unique citrus species (Citrus australasica)	184:228	In this study, a unique citrus species (Citrus australasica) was selected, and its fruit characteristics, phenolic compounds and ability to inhibit inflammation were preliminarily studied.					
30978372	1	57	theme	citrus	193:198	arg1	australasica					216:227	Citrus australasica	209:227	Citrus australasica	209:227	In this study, a unique citrus species (Citrus australasica) was selected, and its fruit characteristics, phenolic compounds and ability to inhibit inflammation were preliminarily studied.					
30978372	7	58	theme	finger	1023:1028	arg1	limes					1030:1034	finger limes	1023:1034	finger limes	1023:1034	Results illustrated that finger limes inhibited the NO-releasing and the inflammation-related cytokines including IL-1β, IL-6 and TNFα elevation.					
30978372	9	59	dep	characteristics	1373:1387	arg1	the					1354:1356	the	1354:1356	the	1354:1356	This study may expand our knowledge on the physiochemical characteristics and bioactive properties of citrus fruits.					
30978372	4	60	theme	finger	690:695	arg1	limes					697:701	finger limes	690:701	finger limes	690:701	The phenolics composition of finger limes showed cultivar and tissue specificity.					
30978372	0	61	from	inhibition	111:120	arg1	species					58:64	a unique citrus species	42:64	a unique citrus species	42:64	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	61	from	inhibition	111:120	arg1	lime					74:77	finger lime	67:77	finger lime (Citrus australasica)	67:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	0	61	from	inhibition	111:120	arg1	line					163:166	BV-2 cell line	153:166	BV-2 cell line	153:166	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	5	62	theme	antioxidant	844:854	arg1	capacities					856:865	better antioxidant capacities	837:865	better antioxidant capacities	837:865	Antioxidant evaluation showed that extracts from finger lime cultivar of 'XiangBin' exhibited better antioxidant capacities than cultivar of 'LiSiKe', especially in peel.					
30978372	2	63	theme	solids	451:456	arg1	contents					497:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents	408:504	Finger lime fruits showed distinctive features in shape, size, weight, colour, total soluble solids, water-soluble pectin, sugar and acids contents.					
30978372	0	64	theme	phenolic	18:25	arg1	compounds					27:35	phenolic compounds	18:35	phenolic compounds from a unique citrus species, finger lime (Citrus australasica)	18:99	Identification of phenolic compounds from a unique citrus species, finger lime (Citrus australasica) and their inhibition of LPS-induced NO-releasing in BV-2 cell line.					
30978372	8	65	theme	LPS-induced	1198:1208	arg1	upregulation					1210:1221	LPS-induced upregulation	1198:1221	LPS-induced upregulation of iNOS	1198:1229	QRT-PCR revealed that finger lime extracts alleviated LPS-induced upregulation of iNOS, IL-6, JAK2, TNFα, TLR2, TLR4, IL-1β, NF-κB and LPS-induced downregulation of IκBα.					
30978372	6	66	theme	LPS-induced	914:924	arg1	model					939:943	LPS-induced NO-releasing model	914:943	LPS-induced NO-releasing model	914:943	LPS-induced NO-releasing model was performed in the mouse microglia BV-2 cell line.					
30978372	1	67	theme	Citrus	209:214	arg1	species					200:206	a unique citrus species	184:206	a unique citrus species (Citrus australasica)	184:228	In this study, a unique citrus species (Citrus australasica) was selected, and its fruit characteristics, phenolic compounds and ability to inhibit inflammation were preliminarily studied.					
30978372	1	67	theme	Citrus	209:214	arg1	australasica					216:227	Citrus australasica	209:227	Citrus australasica	209:227	In this study, a unique citrus species (Citrus australasica) was selected, and its fruit characteristics, phenolic compounds and ability to inhibit inflammation were preliminarily studied.					
30541284	3	0	theme	potential	282:290	arg1	roles					292:296	their potential roles	276:296	their potential roles in regulating sugar composition	276:328	However, their potential roles in regulating sugar composition and the "sugar receding" process of longan fruits lacked systematic investigations.					
30541284	4	1	theme	receding	470:477	arg1	rates					479:483	sugar receding rates	464:483	sugar receding rates	464:483	Our results showed that sucrose/hexose ratios and sugar receding rates of longan pulp varied among cultivars.					
30541284	5	2	with	correlation	628:638	arg1	ratio					660:664	sucrose/hexose ratio	645:664	sucrose/hexose ratio	645:664	Analysis of enzymes for sucrose synthesis and cleavage indicated that DlSAI showed the highest negative correlation with sucrose/hexose ratio at both of activity and expression level.					
30541284	7	3	theme	transcriptional	1046:1060	arg1	regulations					1076:1086	transcriptional and enzymatic regulations	1046:1086	transcriptional and enzymatic regulations	1046:1086	In conclusion, DlSAIs act as key factors influencing sucrose/hexose ratio and sugar receding through transcriptional and enzymatic regulations.					
30541284	4	4	theme	longan	488:493	arg1	pulp					495:498	longan pulp	488:498	longan pulp	488:498	Our results showed that sucrose/hexose ratios and sugar receding rates of longan pulp varied among cultivars.					
30541284	8	5	theme	preserved	1145:1153	arg1	longan					1155:1160	on-tree preserved longan	1137:1160	on-tree preserved longan	1137:1160	These results might help improve the quality of on-tree preserved longan.					
30541284	6	6	from	loss	876:879	arg1	fruits					909:914	'Shixia' longan fruits	893:914	'Shixia' longan fruits	893:914	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	5	7	theme	sucrose/hexose	645:658	arg1	ratio					660:664	sucrose/hexose ratio	645:664	sucrose/hexose ratio	645:664	Analysis of enzymes for sucrose synthesis and cleavage indicated that DlSAI showed the highest negative correlation with sucrose/hexose ratio at both of activity and expression level.					
30541284	6	8	theme	mature	845:850	arg1	stages					852:857	mature stages	845:857	mature stages	845:857	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	0	9	theme	Sugar	84:88	arg1	Receding					90:97	Sugar Receding	84:97	Sugar Receding	84:97	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	2	10	theme	fruit	252:256	arg1	quality					258:264	fruit quality	252:264	fruit quality	252:264	Soluble acid invertases (SAIs) cleave sucrose into hexose in vacuoles and play important roles in influencing fruit quality.					
30541284	3	11	theme	sugar	312:316	arg1	composition					318:328	sugar composition	312:328	sugar composition	312:328	However, their potential roles in regulating sugar composition and the "sugar receding" process of longan fruits lacked systematic investigations.					
30541284	4	12	theme	sucrose/hexose	438:451	arg1	ratios					453:458	sucrose/hexose ratios	438:458	sucrose/hexose ratios	438:458	Our results showed that sucrose/hexose ratios and sugar receding rates of longan pulp varied among cultivars.					
30541284	6	13	theme	sucrose/hexose	783:796	arg1	ratio					798:802	extremely low sucrose/hexose ratio	769:802	extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation	769:942	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	14	from	ratio	798:802	arg1	longan					818:823	'Luosanmu' longan	807:823	'Luosanmu' longan from development to mature stages	807:857	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	14	from	ratio	798:802	arg1	loss					876:879	a remarkable loss	863:879	a remarkable loss of sugar in 'Shixia' longan fruits	863:914	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	14	from	ratio	798:802	arg1	development					830:840	development	830:840	development	830:840	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	8	15	theme	longan	1155:1160	arg1	quality					1126:1132	the quality	1122:1132	the quality of on-tree preserved longan	1122:1160	These results might help improve the quality of on-tree preserved longan.					
30541284	4	16	theme	sugar	464:468	arg1	rates					479:483	sugar receding rates	464:483	sugar receding rates	464:483	Our results showed that sucrose/hexose ratios and sugar receding rates of longan pulp varied among cultivars.					
30541284	0	17	theme	Key	31:33	arg1	Invertases					13:22	Soluble Acid Invertases	0:22	Soluble Acid Invertases	0:22	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	0	17	theme	Key	31:33	arg1	Factors					35:41	Key Factors	31:41	Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan	31:107	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	6	18	theme	low	779:781	arg1	ratio					798:802	extremely low sucrose/hexose ratio	769:802	extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation	769:942	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	0	19	theme	Soluble	0:6	arg1	Invertases					13:22	Soluble Acid Invertases	0:22	Soluble Acid Invertases	0:22	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	0	19	theme	Soluble	0:6	arg1	Factors					35:41	Key Factors	31:41	Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan	31:107	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	2	20	from	sucrose	180:186	arg1	vacuoles					203:210	vacuoles	203:210	vacuoles	203:210	Soluble acid invertases (SAIs) cleave sucrose into hexose in vacuoles and play important roles in influencing fruit quality.					
30541284	0	21	theme	Acid	8:11	arg1	Invertases					13:22	Soluble Acid Invertases	0:22	Soluble Acid Invertases	0:22	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	0	21	theme	Acid	8:11	arg1	Factors					35:41	Key Factors	31:41	Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan	31:107	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	5	22	theme	highest	611:617	arg1	correlation					628:638	the highest negative correlation	607:638	the highest negative correlation with sucrose/hexose ratio	607:664	Analysis of enzymes for sucrose synthesis and cleavage indicated that DlSAI showed the highest negative correlation with sucrose/hexose ratio at both of activity and expression level.					
30541284	5	23	theme	sucrose	548:554	arg1	synthesis					556:564	sucrose synthesis	548:564	sucrose synthesis	548:564	Analysis of enzymes for sucrose synthesis and cleavage indicated that DlSAI showed the highest negative correlation with sucrose/hexose ratio at both of activity and expression level.					
30541284	6	24	theme	remarkable	865:874	arg1	loss					876:879	a remarkable loss	863:879	a remarkable loss of sugar in 'Shixia' longan fruits	863:914	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	3	25	theme	"	353:353	arg1	process					355:361	the "sugar receding" process	334:361	the "sugar receding" process of longan fruits	334:378	However, their potential roles in regulating sugar composition and the "sugar receding" process of longan fruits lacked systematic investigations.					
30541284	5	26	theme	negative	619:626	arg1	correlation					628:638	the highest negative correlation	607:638	the highest negative correlation with sucrose/hexose ratio	607:664	Analysis of enzymes for sucrose synthesis and cleavage indicated that DlSAI showed the highest negative correlation with sucrose/hexose ratio at both of activity and expression level.					
30541284	0	27	dep	Ratio	74:78	arg1	the					55:57	the	55:57	the	55:57	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	3	28	theme	systematic	387:396	arg1	investigations					398:411	systematic investigations	387:411	systematic investigations	387:411	However, their potential roles in regulating sugar composition and the "sugar receding" process of longan fruits lacked systematic investigations.					
30541284	3	29	theme	sugar	339:343	arg1	"					353:353	the "sugar receding"	334:353	the "sugar receding" process of longan fruits	334:378	However, their potential roles in regulating sugar composition and the "sugar receding" process of longan fruits lacked systematic investigations.					
30541284	6	30	theme	on-tree	923:929	arg1	preservation					931:942	on-tree preservation	923:942	on-tree preservation	923:942	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	5	31	theme	expression	690:699	arg1	level					701:705	expression level	690:705	expression level	690:705	Analysis of enzymes for sucrose synthesis and cleavage indicated that DlSAI showed the highest negative correlation with sucrose/hexose ratio at both of activity and expression level.					
30541284	6	32	from	development	830:840	arg1	longan					818:823	'Luosanmu' longan	807:823	'Luosanmu' longan from development to mature stages	807:857	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	32	from	development	830:840	arg1	ratio					798:802	extremely low sucrose/hexose ratio	769:802	extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation	769:942	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	32	from	development	830:840	arg1	loss					876:879	a remarkable loss	863:879	a remarkable loss of sugar in 'Shixia' longan fruits	863:914	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	3	33	theme	longan	366:371	arg1	fruits					373:378	longan fruits	366:378	longan fruits	366:378	However, their potential roles in regulating sugar composition and the "sugar receding" process of longan fruits lacked systematic investigations.					
30541284	7	34	theme	sugar	1023:1027	arg1	receding					1029:1036	sugar receding	1023:1036	sugar receding	1023:1036	In conclusion, DlSAIs act as key factors influencing sucrose/hexose ratio and sugar receding through transcriptional and enzymatic regulations.					
30541284	0	35	from	Ratio	74:78	arg1	Longan					102:107	Longan	102:107	Longan	102:107	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	3	36	theme	fruits	373:378	arg1	roles					292:296	their potential roles	276:296	their potential roles in regulating sugar composition	276:328	However, their potential roles in regulating sugar composition and the "sugar receding" process of longan fruits lacked systematic investigations.					
30541284	3	36	theme	fruits	373:378	arg1	process					355:361	the "sugar receding" process	334:361	the "sugar receding" process of longan fruits	334:378	However, their potential roles in regulating sugar composition and the "sugar receding" process of longan fruits lacked systematic investigations.					
30541284	2	37	theme	acid	150:153	arg1	invertases					155:164	Soluble acid invertases	142:164	Soluble acid invertases (SAIs)	142:171	Soluble acid invertases (SAIs) cleave sucrose into hexose in vacuoles and play important roles in influencing fruit quality.					
30541284	2	37	theme	acid	150:153	arg1	SAIs					167:170	SAIs	167:170	SAIs	167:170	Soluble acid invertases (SAIs) cleave sucrose into hexose in vacuoles and play important roles in influencing fruit quality.					
30541284	7	38	theme	key	974:976	arg1	DlSAIs					960:965	DlSAIs	960:965	DlSAIs	960:965	In conclusion, DlSAIs act as key factors influencing sucrose/hexose ratio and sugar receding through transcriptional and enzymatic regulations.					
30541284	7	38	theme	key	974:976	arg1	factors					978:984	key factors	974:984	key factors influencing sucrose/hexose ratio and sugar receding through transcriptional and enzymatic regulations	974:1086	In conclusion, DlSAIs act as key factors influencing sucrose/hexose ratio and sugar receding through transcriptional and enzymatic regulations.					
30541284	4	39	theme	pulp	495:498	arg1	rates					479:483	sugar receding rates	464:483	sugar receding rates	464:483	Our results showed that sucrose/hexose ratios and sugar receding rates of longan pulp varied among cultivars.					
30541284	4	39	theme	pulp	495:498	arg1	ratios					453:458	sucrose/hexose ratios	438:458	sucrose/hexose ratios	438:458	Our results showed that sucrose/hexose ratios and sugar receding rates of longan pulp varied among cultivars.					
30541284	2	40	theme	Soluble	142:148	arg1	invertases					155:164	Soluble acid invertases	142:164	Soluble acid invertases (SAIs)	142:171	Soluble acid invertases (SAIs) cleave sucrose into hexose in vacuoles and play important roles in influencing fruit quality.					
30541284	2	40	theme	Soluble	142:148	arg1	SAIs					167:170	SAIs	167:170	SAIs	167:170	Soluble acid invertases (SAIs) cleave sucrose into hexose in vacuoles and play important roles in influencing fruit quality.					
30541284	6	41	theme	DlSAI	740:744	arg1	expression					746:755	DlSAI expression	740:755	DlSAI expression	740:755	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	42	from	longan	818:823	arg1	fruits					909:914	'Shixia' longan fruits	893:914	'Shixia' longan fruits	893:914	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	43	theme	sugar	884:888	arg1	longan					818:823	'Luosanmu' longan	807:823	'Luosanmu' longan from development to mature stages	807:857	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	43	theme	sugar	884:888	arg1	loss					876:879	a remarkable loss	863:879	a remarkable loss of sugar in 'Shixia' longan fruits	863:914	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	44	theme	Shixia	894:899	arg1	fruits					909:914	'Shixia' longan fruits	893:914	'Shixia' longan fruits	893:914	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	8	45	theme	on-tree	1137:1143	arg1	longan					1155:1160	on-tree preserved longan	1137:1160	on-tree preserved longan	1137:1160	These results might help improve the quality of on-tree preserved longan.					
30541284	0	46	dep	Act	24:26	arg1	Lour					129:132	Lour	129:132	Lour	129:132	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	5	47	theme	enzymes	536:542	arg1	Analysis					524:531	Analysis	524:531	Analysis of enzymes for sucrose synthesis and cleavage	524:577	Analysis of enzymes for sucrose synthesis and cleavage indicated that DlSAI showed the highest negative correlation with sucrose/hexose ratio at both of activity and expression level.					
30541284	7	48	theme	sucrose/hexose	998:1011	arg1	ratio					1013:1017	sucrose/hexose ratio	998:1017	sucrose/hexose ratio	998:1017	In conclusion, DlSAIs act as key factors influencing sucrose/hexose ratio and sugar receding through transcriptional and enzymatic regulations.					
30541284	0	49	from	Receding	90:97	arg1	Longan					102:107	Longan	102:107	Longan	102:107	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	2	50	theme	important	221:229	arg1	roles					231:235	important roles	221:235	important roles	221:235	Soluble acid invertases (SAIs) cleave sucrose into hexose in vacuoles and play important roles in influencing fruit quality.					
30541284	6	51	theme	SAI	723:725	arg1	activity					727:734	high SAI activity	718:734	high SAI activity	718:734	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	7	52	theme	enzymatic	1066:1074	arg1	regulations					1076:1086	transcriptional and enzymatic regulations	1046:1086	transcriptional and enzymatic regulations	1046:1086	In conclusion, DlSAIs act as key factors influencing sucrose/hexose ratio and sugar receding through transcriptional and enzymatic regulations.					
30541284	0	53	theme	Sucrose/Hexose	59:72	arg1	Ratio					74:78	Sucrose/Hexose Ratio	59:78	Sucrose/Hexose Ratio	59:78	Soluble Acid Invertases Act as Key Factors Influencing the Sucrose/Hexose Ratio and Sugar Receding in Longan ( Dimocarpus longan Lour.)					
30541284	6	54	theme	Luosanmu	808:815	arg1	longan					818:823	'Luosanmu' longan	807:823	'Luosanmu' longan from development to mature stages	807:857	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	6	55	theme	high	718:721	arg1	activity					727:734	high SAI activity	718:734	high SAI activity	718:734	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
30541284	3	56	theme	receding	345:352	arg1	"					353:353	the "sugar receding"	334:353	the "sugar receding" process of longan fruits	334:378	However, their potential roles in regulating sugar composition and the "sugar receding" process of longan fruits lacked systematic investigations.					
30541284	6	57	theme	longan	902:907	arg1	fruits					909:914	'Shixia' longan fruits	893:914	'Shixia' longan fruits	893:914	Moreover, high SAI activity and DlSAI expression resulted in extremely low sucrose/hexose ratio in 'Luosanmu' longan from development to mature stages and a remarkable loss of sugar in 'Shixia' longan fruits during on-tree preservation.					
31562906	13	0	theme	adult	1822:1826	arg1	duct					1846:1849	the neonatal and adult extrahepatic bile duct	1805:1849	the neonatal and adult extrahepatic bile duct	1805:1849	CONCLUSION We identified 4 key differences between the neonatal and adult extrahepatic bile duct.					
31562906	11	1	theme	bile	1631:1634	arg1	diffusion					1618:1626	increased diffusion	1608:1626	increased diffusion of bile	1608:1634	In vitro modeling of the matrix suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile.					
31562906	5	2	theme	extrahepatic	658:669	arg1	ducts					676:680	isolated extrahepatic bile ducts	649:680	isolated extrahepatic bile ducts	649:680	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	10	3	theme	little	1404:1409	arg1	collagen					1411:1418	little collagen I	1404:1420	little collagen I	1404:1420	In contrast, the neonatal submucosa had little collagen I and elastin, although both increased rapidly after birth.					
31562906	3	4	theme	animal	307:312	arg1	models					314:319	Current animal models	299:319	Current animal models	299:319	Current animal models suggest that the developing bile duct is uniquely susceptible to damage.					
31562906	8	5	theme	neonatal	1177:1184	arg1	monolayers					1200:1209	neonatal cholangiocyte monolayers	1177:1209	neonatal cholangiocyte monolayers	1177:1209	Functional studies showed that the glycocalyx protected against bile acid injury and that neonatal cholangiocyte monolayers were more permeable than adult monolayers.					
31562906	7	6	theme	neonatal	939:946	arg1	duct					966:969	the neonatal extrahepatic bile duct	935:969	the neonatal extrahepatic bile duct	935:969	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	13	7	theme	key	1781:1783	arg1	differences					1785:1795	4 key differences	1779:1795	4 key differences between the neonatal and adult extrahepatic bile duct	1779:1849	CONCLUSION We identified 4 key differences between the neonatal and adult extrahepatic bile duct.					
31562906	6	8	theme	uptake	817:822	arg1	assays					824:829	rhodamine uptake assays	807:829	rhodamine uptake assays	807:829	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	11	9	theme	relative	1569:1576	arg1	submucosa					1559:1567	the neonatal submucosa	1546:1567	the neonatal submucosa relative to the adult submucosa	1546:1599	In vitro modeling of the matrix suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile.					
31562906	11	10	theme	neonatal	1550:1557	arg1	submucosa					1559:1567	the neonatal submucosa	1546:1567	the neonatal submucosa relative to the adult submucosa	1546:1599	In vitro modeling of the matrix suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile.					
31562906	5	11	theme	passaged	557:564	arg1	cholangiocytes					566:579	mouse passaged cholangiocytes	551:579	mouse passaged cholangiocytes	551:579	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	9	12	theme	adult	1257:1261	arg1	ducts					1263:1267	adult ducts	1257:1267	adult ducts	1257:1267	In adult ducts, the submucosal space was filled with collagen I, elastin, hyaluronic acid, and proteoglycans.					
31562906	15	13	theme	liver	2164:2168	arg1	injury					2170:2175	liver injury	2164:2175	liver injury	2164:2175	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	4	14	theme	anatomical	432:441	arg1	differences					458:468	the anatomical and functional differences	428:468	the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts	428:529	In this study, we aimed to define the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts.					
31562906	7	15	theme	adult	1065:1069	arg1	cholangiocytes					1071:1084	adult cholangiocytes	1065:1084	adult cholangiocytes	1065:1084	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	2	16	theme	Biliary	227:233	arg1	atresia					235:241	Biliary atresia	227:241	Biliary atresia	227:241	Biliary atresia is a cholangiopathy which exclusively affects neonates.					
31562906	2	16	theme	Biliary	227:233	arg1	cholangiopathy					248:261	a cholangiopathy	246:261	a cholangiopathy which exclusively affects neonates	246:296	Biliary atresia is a cholangiopathy which exclusively affects neonates.					
31562906	1	17	theme	biliary	210:216	arg1	atresia					218:224	biliary atresia	210:224	biliary atresia	210:224	BACKGROUND & AIMS The extrahepatic bile duct is the primary tissue initially affected by biliary atresia.					
31562906	4	18	theme	functional	447:456	arg1	differences					458:468	the anatomical and functional differences	428:468	the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts	428:529	In this study, we aimed to define the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts.					
31562906	7	19	theme	typical	1054:1060	arg1	glycocalyx					1012:1021	uniform apical glycocalyx	997:1021	uniform apical glycocalyx	997:1021	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	9	20	theme	submucosal	1274:1283	arg1	space					1285:1289	the submucosal space	1270:1289	the submucosal space	1270:1289	In adult ducts, the submucosal space was filled with collagen I, elastin, hyaluronic acid, and proteoglycans.					
31562906	7	21	theme	uniform	997:1003	arg1	glycocalyx					1012:1021	uniform apical glycocalyx	997:1021	uniform apical glycocalyx	997:1021	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	1	22	theme	BACKGROUND	121:130	arg1	&					132:132	BACKGROUND &	121:132	BACKGROUND &	121:132	BACKGROUND & AIMS The extrahepatic bile duct is the primary tissue initially affected by biliary atresia.					
31562906	3	23	theme	Current	299:305	arg1	models					314:319	Current animal models	299:319	Current animal models	299:319	Current animal models suggest that the developing bile duct is uniquely susceptible to damage.					
31562906	16	24	theme	neonatal	2337:2344	arg1	duct					2346:2349	the neonatal duct	2333:2349	the neonatal duct	2333:2349	We identify 4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.					
31562906	16	25	theme	duct	2297:2300	arg1	layers					2246:2251	the epithelial and submucosal layers	2216:2251	the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct	2216:2300	We identify 4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.					
31562906	5	26	theme	collagen	689:696	arg1	mouse					707:711	a collagen reporter mouse	687:711	a collagen reporter mouse	687:711	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	3	27	theme	developing	338:347	arg1	duct					354:357	the developing bile duct	334:357	the developing bile duct	334:357	Current animal models suggest that the developing bile duct is uniquely susceptible to damage.					
31562906	3	27	theme	developing	338:347	arg1	susceptible					371:381	susceptible	371:381	susceptible	371:381	Current animal models suggest that the developing bile duct is uniquely susceptible to damage.					
31562906	1	28	theme	extrahepatic	143:154	arg1	tissue					181:186	the primary tissue	169:186	the primary tissue initially affected by biliary atresia	169:224	BACKGROUND & AIMS The extrahepatic bile duct is the primary tissue initially affected by biliary atresia.					
31562906	1	28	theme	extrahepatic	143:154	arg1	duct					161:164	The extrahepatic bile duct	139:164	The extrahepatic bile duct	139:164	BACKGROUND & AIMS The extrahepatic bile duct is the primary tissue initially affected by biliary atresia.					
31562906	4	29	theme	adult	495:499	arg1	ducts					525:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	In this study, we aimed to define the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts.					
31562906	7	30	theme	cell-cell	1034:1042	arg1	junctions					1044:1052	mature cell-cell junctions	1027:1052	mature cell-cell junctions	1027:1052	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	7	31	theme	duct	966:969	arg1	monolayer					922:930	The cholangiocyte monolayer	904:930	The cholangiocyte monolayer of the neonatal extrahepatic bile duct	904:969	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	7	31	theme	duct	966:969	arg1	immature					975:982	immature	975:982	immature	975:982	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	12	32	theme	neonatal	1687:1694	arg1	submucosa					1710:1718	the neonatal but not adult submucosa	1683:1718	the neonatal but not adult submucosa	1683:1718	A Col-GFP reporter mouse showed that cells in the neonatal but not adult submucosa were actively producing collagen.					
31562906	4	33	theme	extrahepatic	507:518	arg1	ducts					525:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	In this study, we aimed to define the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts.					
31562906	11	34	theme	matrix	1505:1510	arg1	modeling					1489:1496	In vitro modeling	1480:1496	In vitro modeling of the matrix	1480:1510	In vitro modeling of the matrix suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile.					
31562906	14	35	contain	have	1886:1889	arg1	features					1873:1880	these features	1867:1880	these features	1867:1880	We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.					
31562906	14	35	contain	have	1886:1889	arg2	implications					1902:1913	functional implications	1891:1913	functional implications	1891:1913	We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.					
31562906	13	36	dep	CONCLUSION	1754:1763	arg1	identified					1768:1777	identified	1768:1777	identified 4 key differences between the neonatal and adult extrahepatic bile duct	1768:1849	CONCLUSION We identified 4 key differences between the neonatal and adult extrahepatic bile duct.					
31562906	5	37	theme	primary	614:620	arg1	cholangiocytes					622:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	0	38	theme	biliary	105:111	arg1	injury					113:118	biliary injury	105:118	biliary injury	105:118	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.					
31562906	9	39	theme	hyaluronic	1328:1337	arg1	acid					1339:1342	hyaluronic acid	1328:1342	hyaluronic acid	1328:1342	In adult ducts, the submucosal space was filled with collagen I, elastin, hyaluronic acid, and proteoglycans.					
31562906	7	40	theme	extrahepatic	948:959	arg1	duct					966:969	the neonatal extrahepatic bile duct	935:969	the neonatal extrahepatic bile duct	935:969	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	4	41	theme	neonatal	482:489	arg1	ducts					525:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	In this study, we aimed to define the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts.					
31562906	0	42	theme	Coordinated	0:10	arg1	development					12:22	Coordinated development	0:22	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.	0:119	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.					
31562906	5	43	theme	BALB/c	588:593	arg1	cholangiocytes					622:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	15	44	theme	Biliary	2029:2035	arg1	disease					2050:2056	a disease	2048:2056	a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury	2048:2175	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	15	44	theme	Biliary	2029:2035	arg1	atresia					2037:2043	LAY SUMMARY Biliary atresia	2017:2043	LAY SUMMARY Biliary atresia	2017:2043	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	10	45	theme	neonatal	1381:1388	arg1	submucosa					1390:1398	the neonatal submucosa	1377:1398	the neonatal submucosa	1377:1398	In contrast, the neonatal submucosa had little collagen I and elastin, although both increased rapidly after birth.					
31562906	13	46	theme	bile	1841:1844	arg1	duct					1846:1849	the neonatal and adult extrahepatic bile duct	1805:1849	the neonatal and adult extrahepatic bile duct	1805:1849	CONCLUSION We identified 4 key differences between the neonatal and adult extrahepatic bile duct.					
31562906	8	47	theme	Functional	1087:1096	arg1	studies					1098:1104	Functional studies	1087:1104	Functional studies	1087:1104	Functional studies showed that the glycocalyx protected against bile acid injury and that neonatal cholangiocyte monolayers were more permeable than adult monolayers.					
31562906	12	48	theme	Col-GFP	1639:1645	arg1	mouse					1656:1660	A Col-GFP reporter mouse	1637:1660	A Col-GFP reporter mouse	1637:1660	A Col-GFP reporter mouse showed that cells in the neonatal but not adult submucosa were actively producing collagen.					
31562906	15	49	theme	LAY	2017:2019	arg1	disease					2050:2056	a disease	2048:2056	a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury	2048:2175	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	15	49	theme	LAY	2017:2019	arg1	atresia					2037:2043	LAY SUMMARY Biliary atresia	2017:2043	LAY SUMMARY Biliary atresia	2017:2043	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	16	50	theme	extrahepatic	2279:2290	arg1	duct					2297:2300	the neonatal and adult extrahepatic bile duct	2256:2300	the neonatal and adult extrahepatic bile duct	2256:2300	We identify 4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.					
31562906	0	51	theme	mouse	31:35	arg1	duct					55:58	the mouse extrahepatic bile duct	27:58	the mouse extrahepatic bile duct	27:58	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.					
31562906	0	52	theme	bile	50:53	arg1	duct					55:58	the mouse extrahepatic bile duct	27:58	the mouse extrahepatic bile duct	27:58	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.					
31562906	6	53	dep	in	863:864	arg1	vitro					866:870	vitro	866:870	vitro	866:870	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	11	54	theme	adult	1585:1589	arg1	submucosa					1591:1599	the adult submucosa	1581:1599	the adult submucosa	1581:1599	In vitro modeling of the matrix suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile.					
31562906	7	55	theme	cholangiocyte	908:920	arg1	monolayer					922:930	The cholangiocyte monolayer	904:930	The cholangiocyte monolayer of the neonatal extrahepatic bile duct	904:969	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	7	55	theme	cholangiocyte	908:920	arg1	immature					975:982	immature	975:982	immature	975:982	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	6	56	theme	acid	837:840	arg1	assays					851:856	bile acid toxicity assays	832:856	bile acid toxicity assays	832:856	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	14	57	theme	bile	1953:1956	arg1	ducts					1958:1962	the neonatal extrahepatic bile ducts	1927:1962	the neonatal extrahepatic bile ducts	1927:1962	We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.					
31562906	14	57	theme	bile	1953:1956	arg1	susceptible					1981:1991	susceptible	1981:1991	susceptible	1981:1991	We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.					
31562906	16	58	theme	key	2192:2194	arg1	differences					2196:2206	4 key differences	2190:2206	4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct	2190:2300	We identify 4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.					
31562906	10	59	contain	had	1400:1402	arg2	elastin					1426:1432	elastin	1426:1432	elastin	1426:1432	In contrast, the neonatal submucosa had little collagen I and elastin, although both increased rapidly after birth.					
31562906	10	59	contain	had	1400:1402	arg1	submucosa					1390:1398	the neonatal submucosa	1377:1398	the neonatal submucosa	1377:1398	In contrast, the neonatal submucosa had little collagen I and elastin, although both increased rapidly after birth.					
31562906	10	59	contain	had	1400:1402	arg2	collagen					1411:1418	little collagen I	1404:1420	little collagen I	1404:1420	In contrast, the neonatal submucosa had little collagen I and elastin, although both increased rapidly after birth.					
31562906	8	60	theme	bile	1151:1154	arg1	injury					1161:1166	bile acid injury	1151:1166	bile acid injury	1151:1166	Functional studies showed that the glycocalyx protected against bile acid injury and that neonatal cholangiocyte monolayers were more permeable than adult monolayers.					
31562906	14	61	theme	neonatal	1931:1938	arg1	ducts					1958:1962	the neonatal extrahepatic bile ducts	1927:1962	the neonatal extrahepatic bile ducts	1927:1962	We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.					
31562906	14	61	theme	neonatal	1931:1938	arg1	susceptible					1981:1991	susceptible	1981:1991	susceptible	1981:1991	We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.					
31562906	5	62	theme	isolated	649:656	arg1	ducts					676:680	isolated extrahepatic bile ducts	649:680	isolated extrahepatic bile ducts	649:680	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	11	63	theme	increased	1608:1616	arg1	diffusion					1618:1626	increased diffusion	1608:1626	increased diffusion of bile	1608:1634	In vitro modeling of the matrix suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile.					
31562906	16	64	theme	adult	2273:2277	arg1	duct					2297:2300	the neonatal and adult extrahepatic bile duct	2256:2300	the neonatal and adult extrahepatic bile duct	2256:2300	We identify 4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.					
31562906	16	65	theme	epithelial	2220:2229	arg1	layers					2246:2251	the epithelial and submucosal layers	2216:2251	the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct	2216:2300	We identify 4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.					
31562906	5	66	theme	bile	671:674	arg1	ducts					676:680	isolated extrahepatic bile ducts	649:680	isolated extrahepatic bile ducts	649:680	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	7	67	dep	RESULTS	896:902	arg1	immature					975:982	immature	975:982	immature	975:982	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	7	67	dep	RESULTS	896:902	arg1	monolayer					922:930	The cholangiocyte monolayer	904:930	The cholangiocyte monolayer of the neonatal extrahepatic bile duct	904:969	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	6	68	theme	matrix	888:893	arg1	immunostaining					791:804	immunostaining	791:804	immunostaining	791:804	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	6	68	theme	matrix	888:893	arg1	microscopy					762:771	transmission electron microscopy	740:771	transmission electron microscopy	740:771	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	6	68	theme	matrix	888:893	arg1	modeling					872:879	in vitro modeling	863:879	in vitro modeling of the matrix	863:893	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	6	68	theme	matrix	888:893	arg1	assays					851:856	bile acid toxicity assays	832:856	bile acid toxicity assays	832:856	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	6	68	theme	matrix	888:893	arg1	assays					824:829	rhodamine uptake assays	807:829	rhodamine uptake assays	807:829	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	6	68	theme	matrix	888:893	arg1	staining					781:788	lectin staining	774:788	lectin staining	774:788	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	5	69	dep	passaged	557:564	arg1	mouse					551:555	mouse	551:555	mouse	551:555	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	16	70	theme	submucosal	2235:2244	arg1	layers					2246:2251	the epithelial and submucosal layers	2216:2251	the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct	2216:2300	We identify 4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.					
31562906	6	71	theme	rhodamine	807:815	arg1	assays					824:829	rhodamine uptake assays	807:829	rhodamine uptake assays	807:829	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	8	72	theme	cholangiocyte	1186:1198	arg1	monolayers					1200:1209	neonatal cholangiocyte monolayers	1177:1209	neonatal cholangiocyte monolayers	1177:1209	Functional studies showed that the glycocalyx protected against bile acid injury and that neonatal cholangiocyte monolayers were more permeable than adult monolayers.					
31562906	1	73	theme	primary	173:179	arg1	tissue					181:186	the primary tissue	169:186	the primary tissue initially affected by biliary atresia	169:224	BACKGROUND & AIMS The extrahepatic bile duct is the primary tissue initially affected by biliary atresia.					
31562906	1	73	theme	primary	173:179	arg1	duct					161:164	The extrahepatic bile duct	139:164	The extrahepatic bile duct	139:164	BACKGROUND & AIMS The extrahepatic bile duct is the primary tissue initially affected by biliary atresia.					
31562906	5	74	dep	METHODS	532:538	arg1	studied					543:549	studied	543:549	studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse	543:711	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	7	75	theme	cholangiocytes	1071:1084	arg1	typical					1054:1060	typical	1054:1060	typical	1054:1060	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	11	76	theme	submucosa	1559:1567	arg1	composition					1531:1541	the composition	1527:1541	the composition of the neonatal submucosa relative to the adult submucosa	1527:1599	In vitro modeling of the matrix suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile.					
31562906	14	77	theme	functional	1891:1900	arg1	implications					1902:1913	functional implications	1891:1913	functional implications	1891:1913	We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.					
31562906	8	78	theme	adult	1236:1240	arg1	monolayers					1242:1251	adult monolayers	1236:1251	adult monolayers	1236:1251	Functional studies showed that the glycocalyx protected against bile acid injury and that neonatal cholangiocyte monolayers were more permeable than adult monolayers.					
31562906	5	79	theme	reporter	698:705	arg1	mouse					707:711	a collagen reporter mouse	687:711	a collagen reporter mouse	687:711	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	7	80	theme	mature	1027:1032	arg1	junctions					1044:1052	mature cell-cell junctions	1027:1052	mature cell-cell junctions	1027:1052	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	7	81	theme	apical	1005:1010	arg1	glycocalyx					1012:1021	uniform apical glycocalyx	997:1021	uniform apical glycocalyx	997:1021	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	6	82	theme	electron	753:760	arg1	microscopy					762:771	transmission electron microscopy	740:771	transmission electron microscopy	740:771	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	11	83	theme	In	1480:1481	arg1	modeling					1489:1496	In vitro modeling	1480:1496	In vitro modeling of the matrix	1480:1510	In vitro modeling of the matrix suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile.					
31562906	12	84	theme	adult	1704:1708	arg1	submucosa					1710:1718	the neonatal but not adult submucosa	1683:1718	the neonatal but not adult submucosa	1683:1718	A Col-GFP reporter mouse showed that cells in the neonatal but not adult submucosa were actively producing collagen.					
31562906	3	85	theme	bile	349:352	arg1	duct					354:357	the developing bile duct	334:357	the developing bile duct	334:357	Current animal models suggest that the developing bile duct is uniquely susceptible to damage.					
31562906	3	85	theme	bile	349:352	arg1	susceptible					371:381	susceptible	371:381	susceptible	371:381	Current animal models suggest that the developing bile duct is uniquely susceptible to damage.					
31562906	4	86	theme	mouse	501:505	arg1	ducts					525:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	In this study, we aimed to define the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts.					
31562906	12	87	from	cells	1674:1678	arg1	submucosa					1710:1718	the neonatal but not adult submucosa	1683:1718	the neonatal but not adult submucosa	1683:1718	A Col-GFP reporter mouse showed that cells in the neonatal but not adult submucosa were actively producing collagen.					
31562906	0	88	theme	neonatal	78:85	arg1	susceptibility					87:100	neonatal susceptibility	78:100	neonatal susceptibility to biliary injury	78:118	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.					
31562906	1	89	theme	bile	156:159	arg1	tissue					181:186	the primary tissue	169:186	the primary tissue initially affected by biliary atresia	169:224	BACKGROUND & AIMS The extrahepatic bile duct is the primary tissue initially affected by biliary atresia.					
31562906	1	89	theme	bile	156:159	arg1	duct					161:164	The extrahepatic bile duct	139:164	The extrahepatic bile duct	139:164	BACKGROUND & AIMS The extrahepatic bile duct is the primary tissue initially affected by biliary atresia.					
31562906	4	90	theme	bile	520:523	arg1	ducts					525:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	the neonatal and adult mouse extrahepatic bile ducts	478:529	In this study, we aimed to define the anatomical and functional differences between the neonatal and adult mouse extrahepatic bile ducts.					
31562906	11	91	dep	In	1480:1481	arg1	vitro					1483:1487	vitro	1483:1487	vitro	1483:1487	In vitro modeling of the matrix suggested that the composition of the neonatal submucosa relative to the adult submucosa led to increased diffusion of bile.					
31562906	15	92	theme	bile	2117:2120	arg1	injury					2127:2132	extrahepatic bile duct injury	2104:2132	extrahepatic bile duct injury	2104:2132	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	5	93	theme	adult	608:612	arg1	cholangiocytes					622:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	7	94	theme	bile	961:964	arg1	duct					966:969	the neonatal extrahepatic bile duct	935:969	the neonatal extrahepatic bile duct	935:969	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	12	95	theme	reporter	1647:1654	arg1	mouse					1656:1660	A Col-GFP reporter mouse	1637:1660	A Col-GFP reporter mouse	1637:1660	A Col-GFP reporter mouse showed that cells in the neonatal but not adult submucosa were actively producing collagen.					
31562906	7	96	dep	glycocalyx	1012:1021	arg1	the					993:995	the	993:995	the	993:995	RESULTS The cholangiocyte monolayer of the neonatal extrahepatic bile duct was immature, lacking the uniform apical glycocalyx and mature cell-cell junctions typical of adult cholangiocytes.					
31562906	5	97	theme	mouse	582:586	arg1	cholangiocytes					622:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	13	98	theme	extrahepatic	1828:1839	arg1	duct					1846:1849	the neonatal and adult extrahepatic bile duct	1805:1849	the neonatal and adult extrahepatic bile duct	1805:1849	CONCLUSION We identified 4 key differences between the neonatal and adult extrahepatic bile duct.					
31562906	14	99	theme	extrahepatic	1940:1951	arg1	ducts					1958:1962	the neonatal extrahepatic bile ducts	1927:1962	the neonatal extrahepatic bile ducts	1927:1962	We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.					
31562906	14	99	theme	extrahepatic	1940:1951	arg1	susceptible					1981:1991	susceptible	1981:1991	susceptible	1981:1991	We showed that these features may have functional implications, suggesting the neonatal extrahepatic bile ducts are particularly susceptible to injury and fibrosis.					
31562906	5	100	theme	neonatal	595:602	arg1	cholangiocytes					622:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	mouse BALB/c neonatal and adult primary cholangiocytes	582:635	METHODS We studied mouse passaged cholangiocytes, mouse BALB/c neonatal and adult primary cholangiocytes, as well as isolated extrahepatic bile ducts, and a collagen reporter mouse.					
31562906	16	101	theme	bile	2292:2295	arg1	duct					2297:2300	the neonatal and adult extrahepatic bile duct	2256:2300	the neonatal and adult extrahepatic bile duct	2256:2300	We identify 4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.					
31562906	0	102	theme	extrahepatic	37:48	arg1	duct					55:58	the mouse extrahepatic bile duct	27:58	the mouse extrahepatic bile duct	27:58	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.					
31562906	6	103	theme	in	863:864	arg1	modeling					872:879	in vitro modeling	863:879	in vitro modeling of the matrix	863:893	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	15	104	theme	SUMMARY	2021:2027	arg1	disease					2050:2056	a disease	2048:2056	a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury	2048:2175	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	15	104	theme	SUMMARY	2021:2027	arg1	atresia					2037:2043	LAY SUMMARY Biliary atresia	2017:2043	LAY SUMMARY Biliary atresia	2017:2043	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	6	105	theme	lectin	774:779	arg1	staining					781:788	lectin staining	774:788	lectin staining	774:788	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	0	106	theme	duct	55:58	arg1	development					12:22	Coordinated development	0:22	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.	0:119	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.					
31562906	15	107	theme	duct	2122:2125	arg1	injury					2127:2132	extrahepatic bile duct injury	2104:2132	extrahepatic bile duct injury	2104:2132	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	6	108	theme	toxicity	842:849	arg1	assays					851:856	bile acid toxicity assays	832:856	bile acid toxicity assays	832:856	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	15	109	theme	extrahepatic	2104:2115	arg1	injury					2127:2132	extrahepatic bile duct injury	2104:2132	extrahepatic bile duct injury	2104:2132	LAY SUMMARY Biliary atresia is a disease that affects newborns and is characterized by extrahepatic bile duct injury and obstruction, resulting in liver injury.					
31562906	6	110	theme	transmission	740:751	arg1	microscopy					762:771	transmission electron microscopy	740:771	transmission electron microscopy	740:771	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	16	111	theme	neonatal	2260:2267	arg1	duct					2297:2300	the neonatal and adult extrahepatic bile duct	2256:2300	the neonatal and adult extrahepatic bile duct	2256:2300	We identify 4 key differences between the epithelial and submucosal layers of the neonatal and adult extrahepatic bile duct and show that these may render the neonatal duct particularly susceptible to injury.					
31562906	8	112	theme	acid	1156:1159	arg1	injury					1161:1166	bile acid injury	1151:1166	bile acid injury	1151:1166	Functional studies showed that the glycocalyx protected against bile acid injury and that neonatal cholangiocyte monolayers were more permeable than adult monolayers.					
31562906	6	113	theme	bile	832:835	arg1	assays					851:856	bile acid toxicity assays	832:856	bile acid toxicity assays	832:856	The methods used included transmission electron microscopy, lectin staining, immunostaining, rhodamine uptake assays, bile acid toxicity assays, and in vitro modeling of the matrix.					
31562906	0	114	dep	development	12:22	arg1	Implications					61:72	Implications	61:72	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.	0:119	Coordinated development of the mouse extrahepatic bile duct: Implications for neonatal susceptibility to biliary injury.					
31562906	13	115	theme	neonatal	1809:1816	arg1	duct					1846:1849	the neonatal and adult extrahepatic bile duct	1805:1849	the neonatal and adult extrahepatic bile duct	1805:1849	CONCLUSION We identified 4 key differences between the neonatal and adult extrahepatic bile duct.					
31677079	3	0	theme	experimental	529:540	arg1	method					549:554	Box-Behnken experimental design method	517:554	Box-Behnken experimental design method with three independent factors	517:585	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	12	1	theme	HNa-Ind	1979:1985	arg1	NE					1987:1988	HNa-Ind NE	1979:1988	HNa-Ind NE	1979:1988	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	12	1	theme	HNa-Ind	1979:1985	arg1	carrier					2026:2032	a successful carrier	2013:2032	a successful carrier with enhanced antioxidant and anti-inflammatory potential	2013:2090	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	11	2	theme	drug	1916:1919	arg1	capability					1932:1941	drug deposition capability	1916:1941	drug deposition capability	1916:1941	The optimized HNa-Ind NE formulation showed considerably higher skin permeation and drug deposition capability compared with the HNa-Ind solution.					
31677079	7	3	theme	antioxidant	1105:1115	arg1	ability					1117:1123	the antioxidant ability	1101:1123	the antioxidant ability of the two drugs	1101:1140	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	3	4	theme	Box-Behnken	517:527	arg1	method					549:554	Box-Behnken experimental design method	517:554	Box-Behnken experimental design method with three independent factors	517:585	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	8	5	from	concentrations	1387:1400	arg1	inhibition					1366:1375	inhibition	1366:1375	94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%	1359:1466	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	1	6	theme	nanoemulsion	175:186	arg1	formula					154:160	the formula	150:160	the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity	150:309	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	12	7	with	carrier	2026:2032	arg1	antioxidant					2048:2058	antioxidant	2048:2058	antioxidant	2048:2058	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	12	7	with	carrier	2026:2032	arg1	potential					2082:2090	enhanced antioxidant and anti-inflammatory potential	2039:2090	potential	2082:2090	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	10	8	theme	mice	1758:1761	arg1	model					1763:1767	mice model	1758:1767	mice model	1758:1767	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	4	9	dep	in	751:752	arg1	vivo					754:757	vivo	754:757	vivo	754:757	The selected formula was tested for its in vitro antioxidant activity and in vivo anti-inflammatory activity.					
31677079	8	10	dep	decreased	1348:1356	arg1	%					1364:1364	94.28%	1359:1364	94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%	1359:1466	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	11	11	theme	HNa-Ind	1846:1852	arg1	formulation					1857:1867	The optimized HNa-Ind NE formulation	1832:1867	The optimized HNa-Ind NE formulation	1832:1867	The optimized HNa-Ind NE formulation showed considerably higher skin permeation and drug deposition capability compared with the HNa-Ind solution.					
31677079	4	12	theme	selected	681:688	arg1	formula					690:696	The selected formula	677:696	The selected formula	677:696	The selected formula was tested for its in vitro antioxidant activity and in vivo anti-inflammatory activity.					
31677079	7	13	theme	optimized	1059:1067	arg1	able					1084:1087	able	1084:1087	able	1084:1087	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	7	13	theme	optimized	1059:1067	arg1	NE					1077:1078	the optimized HNa-Ind NE	1055:1078	the optimized HNa-Ind NE	1055:1078	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	3	14	theme	polydispersity	628:641	arg1	index					643:647	polydispersity index	628:647	polydispersity index	628:647	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	5	15	theme	nm	885:886	arg1	index					904:908	polydispersity index	889:908	polydispersity index of 0.606 ± 0.082	889:925	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	5	15	theme	nm	885:886	arg1	percentage					943:952	99.4 ± 0.1 percentage	932:952	99.4 ± 0.1 percentage of transmittance	932:969	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	5	15	theme	nm	885:886	arg1	size					863:866	a particle size	852:866	a particle size of 12.87 ± 0.032 nm	852:886	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	11	16	theme	deposition	1921:1930	arg1	capability					1932:1941	drug deposition capability	1916:1941	drug deposition capability	1916:1941	The optimized HNa-Ind NE formulation showed considerably higher skin permeation and drug deposition capability compared with the HNa-Ind solution.					
31677079	5	17	theme	99.4	932:935	arg1	±					937:937	±	937:937	±	937:937	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	10	18	theme	ear	1745:1747	arg1	edema					1749:1753	xylene-induced ear edema	1730:1753	xylene-induced ear edema	1730:1753	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	5	19	theme	0.1	939:941	arg1	±					937:937	±	937:937	±	937:937	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	6	20	theme	physical	1024:1031	arg1	interaction					987:997	no interaction	984:997	no interaction between HNa and Ind	984:1017	FTIR showed no interaction between HNa and Ind as a physical mixture.					
31677079	6	20	theme	physical	1024:1031	arg1	mixture					1033:1039	a physical mixture	1022:1039	a physical mixture	1022:1039	FTIR showed no interaction between HNa and Ind as a physical mixture.					
31677079	8	21	theme	%	1466:1466	arg1	inhibition					1449:1458	an inhibition	1446:1458	an inhibition of 100%	1446:1466	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	4	22	theme	in	717:718	arg1	activity					738:745	in vitro antioxidant activity	717:745	in vitro antioxidant activity	717:745	The selected formula was tested for its in vitro antioxidant activity and in vivo anti-inflammatory activity.					
31677079	5	23	theme	HNa-Ind	801:807	arg1	formula					812:818	The optimized HNa-Ind NE formula	787:818	The optimized HNa-Ind NE formula	787:818	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	10	24	theme	inflamed	1783:1790	arg1	region					1792:1797	the inflamed region	1779:1797	the inflamed region reduced by 92.5% upon treatment	1779:1829	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	5	25	theme	±	919:919	arg1	index					904:908	polydispersity index	889:908	polydispersity index of 0.606 ± 0.082	889:925	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	5	25	theme	±	919:919	arg1	percentage					943:952	99.4 ± 0.1 percentage	932:952	99.4 ± 0.1 percentage of transmittance	932:969	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	5	25	theme	±	919:919	arg1	size					863:866	a particle size	852:866	a particle size of 12.87 ± 0.032 nm	852:886	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	4	26	theme	antioxidant	726:736	arg1	activity					738:745	in vitro antioxidant activity	717:745	in vitro antioxidant activity	717:745	The selected formula was tested for its in vitro antioxidant activity and in vivo anti-inflammatory activity.					
31677079	3	27	with	method	549:554	arg1	factors					579:585	three independent factors	561:585	three independent factors	561:585	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	3	28	theme	percent	654:660	arg1	transmittance					662:674	percent transmittance	654:674	percent transmittance	654:674	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	10	29	theme	xylene-induced	1730:1743	arg1	edema					1749:1753	xylene-induced ear edema	1730:1753	xylene-induced ear edema	1730:1753	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	9	30	theme	optimized	1535:1543	arg1	formulation					1556:1566	the optimized HNa-Ind NE formulation	1531:1566	the optimized HNa-Ind NE formulation	1531:1566	Moreover, the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation was assessed using an in vivo model.					
31677079	12	31	theme	anti-inflammatory	2064:2080	arg1	potential					2082:2090	enhanced antioxidant and anti-inflammatory potential	2039:2090	potential	2082:2090	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	10	32	theme	gel	1646:1648	arg1	%					1651:1651	ibuprofen gel 5%	1636:1651	ibuprofen gel 5%	1636:1651	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	10	32	theme	gel	1646:1648	arg1	drugs					1629:1633	reference drugs	1619:1633	reference drugs (ibuprofen gel 5%)	1619:1652	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	0	33	theme	Nanoemulsions	38:50	arg1	Assessment					16:25	Assessment	16:25	Assessment	16:25	Development and Assessment of Lipidic Nanoemulsions Containing Sodium Hyaluronate and Indomethacin.					
31677079	0	33	theme	Nanoemulsions	38:50	arg1	Development					0:10	Development	0:10	Development	0:10	Development and Assessment of Lipidic Nanoemulsions Containing Sodium Hyaluronate and Indomethacin.					
31677079	9	34	theme	NE	1553:1554	arg1	formulation					1556:1566	the optimized HNa-Ind NE formulation	1531:1566	the optimized HNa-Ind NE formulation	1531:1566	Moreover, the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation was assessed using an in vivo model.					
31677079	11	35	theme	NE	1854:1855	arg1	formulation					1857:1867	The optimized HNa-Ind NE formulation	1832:1867	The optimized HNa-Ind NE formulation	1832:1867	The optimized HNa-Ind NE formulation showed considerably higher skin permeation and drug deposition capability compared with the HNa-Ind solution.					
31677079	5	36	theme	particle	854:861	arg1	size					863:866	a particle size	852:866	a particle size of 12.87 ± 0.032 nm	852:886	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	1	37	theme	present	104:110	arg1	work					112:115	The present work	100:115	The present work	100:115	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	10	38	from	region	1792:1797	arg1	model					1763:1767	mice model	1758:1767	mice model	1758:1767	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	7	39	from	able	1084:1087	arg1	addition					1045:1052	addition	1045:1052	addition	1045:1052	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	3	40	theme	optimal	463:469	arg1	formulation					471:481	the optimal formulation	459:481	the optimal formulation	459:481	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	12	41	dep	antioxidant	2048:2058	arg1	enhanced					2039:2046	enhanced	2039:2046	enhanced	2039:2046	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	8	42	theme	free	1311:1314	arg1	scavenging					1324:1333	the free radical scavenging	1307:1333	the free radical scavenging activity	1307:1342	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	12	43	theme	drug	2178:2181	arg1	delivery					2183:2190	transdermal drug delivery	2166:2190	transdermal drug delivery	2166:2190	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	11	44	theme	higher	1889:1894	arg1	permeation					1901:1910	considerably higher skin permeation	1876:1910	considerably higher skin permeation	1876:1910	The optimized HNa-Ind NE formulation showed considerably higher skin permeation and drug deposition capability compared with the HNa-Ind solution.					
31677079	0	45	theme	Lipidic	30:36	arg1	Nanoemulsions					38:50	Lipidic Nanoemulsions	30:50	Lipidic Nanoemulsions	30:50	Development and Assessment of Lipidic Nanoemulsions Containing Sodium Hyaluronate and Indomethacin.					
31677079	1	46	theme	transdermal	276:286	arg1	activity					302:309	enhanced transdermal antiarthritic activity	267:309	enhanced transdermal antiarthritic activity	267:309	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	9	47	theme	in	1590:1591	arg1	model					1598:1602	an in vivo model	1587:1602	an in vivo model	1587:1602	Moreover, the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation was assessed using an in vivo model.					
31677079	12	48	theme	promising	2144:2152	arg1	vehicle					2154:2160	a promising vehicle	2142:2160	a promising vehicle for transdermal drug delivery	2142:2190	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	2	49	dep	infrared	410:417	arg1	FTIR					420:423	FTIR	420:423	FTIR	420:423	NEs were prepared by the spontaneous emulsification method and characterized by Fourier-transform infrared (FTIR) spectroscopy.					
31677079	10	50	theme	remarkable	1659:1668	arg1	activity					1670:1677	the remarkable activity	1655:1677	the remarkable activity of the optimized formulation	1655:1706	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	10	51	theme	optimized	1686:1694	arg1	formulation					1696:1706	the optimized formulation	1682:1706	the optimized formulation	1682:1706	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	9	52	theme	anti-inflammatory	1500:1516	arg1	potential					1518:1526	the pharmacological anti-inflammatory potential	1480:1526	the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation	1480:1566	Moreover, the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation was assessed using an in vivo model.					
31677079	11	53	theme	skin	1896:1899	arg1	permeation					1901:1910	considerably higher skin permeation	1876:1910	considerably higher skin permeation	1876:1910	The optimized HNa-Ind NE formulation showed considerably higher skin permeation and drug deposition capability compared with the HNa-Ind solution.					
31677079	3	54	theme	design	542:547	arg1	method					549:554	Box-Behnken experimental design method	517:554	Box-Behnken experimental design method with three independent factors	517:585	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	5	55	theme	polydispersity	889:902	arg1	index					904:908	polydispersity index	889:908	polydispersity index of 0.606 ± 0.082	889:925	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	7	56	theme	scavenging	1248:1257	arg1	ability					1259:1265	free radical scavenging ability	1235:1265	free radical scavenging ability	1235:1265	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	7	57	theme	inhibition	1203:1212	arg1	assay					1214:1218	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay	1164:1218	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability	1164:1265	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	12	58	theme	transdermal	2166:2176	arg1	delivery					2183:2190	transdermal drug delivery	2166:2190	transdermal drug delivery	2166:2190	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	2	59	theme	spontaneous	337:347	arg1	method					364:369	the spontaneous emulsification method	333:369	the spontaneous emulsification method	333:369	NEs were prepared by the spontaneous emulsification method and characterized by Fourier-transform infrared (FTIR) spectroscopy.					
31677079	7	60	theme	free	1235:1238	arg1	scavenging					1248:1257	free radical scavenging	1235:1257	free radical scavenging ability	1235:1265	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	3	61	theme	independent	567:577	arg1	factors					579:585	three independent factors	561:585	three independent factors	561:585	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	8	62	theme	scavenging	1324:1333	arg1	activity					1335:1342	the free radical scavenging activity	1307:1342	the free radical scavenging activity	1307:1342	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	1	63	theme	lipidic	167:173	arg1	NE					189:190	NE	189:190	NE	189:190	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	1	63	theme	lipidic	167:173	arg1	nanoemulsion					175:186	a lipidic nanoemulsion	165:186	a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind	165:261	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	8	64	dep	%	1364:1364	arg1	inhibition					1366:1375	inhibition	1366:1375	94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%	1359:1466	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	9	65	dep	in	1590:1591	arg1	vivo					1593:1596	vivo	1593:1596	vivo	1593:1596	Moreover, the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation was assessed using an in vivo model.					
31677079	7	66	theme	HNa-Ind	1069:1075	arg1	able					1084:1087	able	1084:1087	able	1084:1087	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	7	66	theme	HNa-Ind	1069:1075	arg1	NE					1077:1078	the optimized HNa-Ind NE	1055:1078	the optimized HNa-Ind NE	1055:1078	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	5	67	theme	12.87	871:875	arg1	±					877:877	±	877:877	±	877:877	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	10	68	theme	reference	1619:1627	arg1	%					1651:1651	ibuprofen gel 5%	1636:1651	ibuprofen gel 5%	1636:1651	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	10	68	theme	reference	1619:1627	arg1	drugs					1629:1633	reference drugs	1619:1633	reference drugs (ibuprofen gel 5%)	1619:1652	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	5	69	theme	0.032	879:883	arg1	±					877:877	±	877:877	±	877:877	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	8	70	theme	cell	1272:1275	arg1	viability					1277:1285	The cell viability	1268:1285	The cell viability	1268:1285	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	12	71	theme	better	2106:2111	arg1	penetration					2118:2128	better skin penetration	2106:2128	better skin penetration	2106:2128	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	3	72	theme	particle	613:620	arg1	size					622:625	particle size	613:625	particle size	613:625	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	11	73	theme	HNa-Ind	1961:1967	arg1	solution					1969:1976	the HNa-Ind solution	1957:1976	the HNa-Ind solution	1957:1976	The optimized HNa-Ind NE formulation showed considerably higher skin permeation and drug deposition capability compared with the HNa-Ind solution.					
31677079	4	74	theme	in	751:752	arg1	activity					777:784	in vivo anti-inflammatory activity	751:784	in vivo anti-inflammatory activity	751:784	The selected formula was tested for its in vitro antioxidant activity and in vivo anti-inflammatory activity.					
31677079	5	75	theme	optimized	791:799	arg1	formula					812:818	The optimized HNa-Ind NE formula	787:818	The optimized HNa-Ind NE formula	787:818	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	9	76	theme	pharmacological	1484:1498	arg1	potential					1518:1526	the pharmacological anti-inflammatory potential	1480:1526	the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation	1480:1566	Moreover, the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation was assessed using an in vivo model.					
31677079	12	77	theme	successful	2015:2024	arg1	NE					1987:1988	HNa-Ind NE	1979:1988	HNa-Ind NE	1979:1988	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	12	77	theme	successful	2015:2024	arg1	carrier					2026:2032	a successful carrier	2013:2032	a successful carrier with enhanced antioxidant and anti-inflammatory potential	2013:2090	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	5	78	theme	NE	809:810	arg1	formula					812:818	The optimized HNa-Ind NE formula	787:818	The optimized HNa-Ind NE formula	787:818	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	8	79	theme	radical	1316:1322	arg1	scavenging					1324:1333	the free radical scavenging	1307:1333	the free radical scavenging activity	1307:1342	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	7	80	theme	DPPH	1197:1200	arg1	assay					1214:1218	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay	1164:1218	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability	1164:1265	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	12	81	theme	skin	2113:2116	arg1	penetration					2118:2128	better skin penetration	2106:2128	better skin penetration	2106:2128	HNa-Ind NE was demonstrated to be a successful carrier with enhanced antioxidant and anti-inflammatory potential while showing better skin penetration, thus being a promising vehicle for transdermal drug delivery.					
31677079	7	82	theme	2,2-diphenyl-1-picrylhydrazyl	1166:1194	arg1	assay					1214:1218	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay	1164:1218	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability	1164:1265	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	4	83	dep	in	717:718	arg1	vitro					720:724	vitro	720:724	vitro	720:724	The selected formula was tested for its in vitro antioxidant activity and in vivo anti-inflammatory activity.					
31677079	10	84	theme	ibuprofen	1636:1644	arg1	%					1651:1651	ibuprofen gel 5%	1636:1651	ibuprofen gel 5%	1636:1651	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	10	84	theme	ibuprofen	1636:1644	arg1	drugs					1629:1633	reference drugs	1619:1633	reference drugs (ibuprofen gel 5%)	1619:1652	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31677079	9	85	theme	formulation	1556:1566	arg1	potential					1518:1526	the pharmacological anti-inflammatory potential	1480:1526	the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation	1480:1566	Moreover, the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation was assessed using an in vivo model.					
31677079	11	86	theme	optimized	1836:1844	arg1	formulation					1857:1867	The optimized HNa-Ind NE formulation	1832:1867	The optimized HNa-Ind NE formulation	1832:1867	The optimized HNa-Ind NE formulation showed considerably higher skin permeation and drug deposition capability compared with the HNa-Ind solution.					
31677079	4	87	theme	anti-inflammatory	759:775	arg1	activity					777:784	in vivo anti-inflammatory activity	751:784	in vivo anti-inflammatory activity	751:784	The selected formula was tested for its in vitro antioxidant activity and in vivo anti-inflammatory activity.					
31677079	5	88	theme	transmittance	957:969	arg1	index					904:908	polydispersity index	889:908	polydispersity index of 0.606 ± 0.082	889:925	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	5	88	theme	transmittance	957:969	arg1	percentage					943:952	99.4 ± 0.1 percentage	932:952	99.4 ± 0.1 percentage of transmittance	932:969	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	5	88	theme	transmittance	957:969	arg1	size					863:866	a particle size	852:866	a particle size of 12.87 ± 0.032 nm	852:886	The optimized HNa-Ind NE formula was characterized and displayed a particle size of 12.87 ± 0.032 nm, polydispersity index of 0.606 ± 0.082, and 99.4 ± 0.1 percentage of transmittance.					
31677079	9	89	theme	HNa-Ind	1545:1551	arg1	formulation					1556:1566	the optimized HNa-Ind NE formulation	1531:1566	the optimized HNa-Ind NE formulation	1531:1566	Moreover, the pharmacological anti-inflammatory potential of the optimized HNa-Ind NE formulation was assessed using an in vivo model.					
31677079	3	90	theme	formulation	471:481	arg1	composition					444:454	The composition	440:454	The composition of the optimal formulation	440:481	The composition of the optimal formulation was statistically optimized using Box-Behnken experimental design method with three independent factors and was characterized for particle size, polydispersity index, and percent transmittance.					
31677079	7	91	theme	drugs	1136:1140	arg1	ability					1117:1123	the antioxidant ability	1101:1123	the antioxidant ability of the two drugs	1101:1140	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	1	92	theme	enhanced	267:274	arg1	activity					302:309	enhanced transdermal antiarthritic activity	267:309	enhanced transdermal antiarthritic activity	267:309	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	1	93	theme	sodium	204:209	arg1	HNa					224:226	HNa	224:226	HNa	224:226	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	1	93	theme	sodium	204:209	arg1	hyaluronate					211:221	sodium hyaluronate	204:221	sodium hyaluronate (HNa)	204:227	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	8	94	theme	higher	1380:1385	arg1	concentrations					1387:1400	higher concentrations	1380:1400	higher concentrations	1380:1400	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	2	95	theme	infrared	410:417	arg1	spectroscopy					426:437	Fourier-transform infrared (FTIR) spectroscopy	392:437	Fourier-transform infrared (FTIR) spectroscopy	392:437	NEs were prepared by the spontaneous emulsification method and characterized by Fourier-transform infrared (FTIR) spectroscopy.					
31677079	0	96	theme	Sodium	63:68	arg1	Hyaluronate					70:80	Sodium Hyaluronate	63:80	Sodium Hyaluronate	63:80	Development and Assessment of Lipidic Nanoemulsions Containing Sodium Hyaluronate and Indomethacin.					
31677079	1	97	theme	antiarthritic	288:300	arg1	activity					302:309	enhanced transdermal antiarthritic activity	267:309	enhanced transdermal antiarthritic activity	267:309	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	2	98	theme	emulsification	349:362	arg1	method					364:369	the spontaneous emulsification method	333:369	the spontaneous emulsification method	333:369	NEs were prepared by the spontaneous emulsification method and characterized by Fourier-transform infrared (FTIR) spectroscopy.					
31677079	1	99	contain	containing	193:202	arg2	hyaluronate					211:221	sodium hyaluronate	204:221	sodium hyaluronate (HNa)	204:227	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	1	99	contain	containing	193:202	arg1	NE					189:190	NE	189:190	NE	189:190	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	1	99	contain	containing	193:202	arg2	indomethacin					233:244	indomethacin	233:244	indomethacin (Ind)	233:250	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	1	99	contain	containing	193:202	arg2	Ind					247:249	Ind	247:249	Ind	247:249	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	1	99	contain	containing	193:202	arg2	HNa					224:226	HNa	224:226	HNa	224:226	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	1	99	contain	containing	193:202	arg1	nanoemulsion					175:186	a lipidic nanoemulsion	165:186	a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind	165:261	The present work attempts to develop and optimize the formula of a lipidic nanoemulsion (NE) containing sodium hyaluronate (HNa) and indomethacin (Ind) as HNa-Ind for enhanced transdermal antiarthritic activity.					
31677079	8	100	theme	vitamin	1416:1422	arg1	C					1424:1424	vitamin C	1416:1424	vitamin C	1416:1424	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	8	101	with	reference	1431:1439	arg1	inhibition					1449:1458	an inhibition	1446:1458	an inhibition of 100%	1446:1466	The cell viability was increased while the free radical scavenging activity was decreased (94.28% inhibition at higher concentrations compared with vitamin C as a reference with an inhibition of 100%).					
31677079	7	102	theme	radical	1240:1246	arg1	scavenging					1248:1257	free radical scavenging	1235:1257	free radical scavenging ability	1235:1265	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	7	103	from	addition	1045:1052	arg1	able					1084:1087	able	1084:1087	able	1084:1087	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	7	103	from	addition	1045:1052	arg1	NE					1077:1078	the optimized HNa-Ind NE	1055:1078	the optimized HNa-Ind NE	1055:1078	In addition, the optimized HNa-Ind NE was able to preserve the antioxidant ability of the two drugs, as evidenced through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) inhibition assay used to assess free radical scavenging ability.					
31677079	10	104	theme	formulation	1696:1706	arg1	activity					1670:1677	the remarkable activity	1655:1677	the remarkable activity of the optimized formulation	1655:1706	Compared with reference drugs (ibuprofen gel 5%), the remarkable activity of the optimized formulation was established using xylene-induced ear edema in mice model, in which the inflamed region reduced by 92.5% upon treatment.					
31636375	8	0	theme	adipose	1141:1147	arg1	tissue					1149:1154	adipose tissue	1141:1154	adipose tissue	1141:1154	RESULTS Both high-fat/high-sucrose diets increased body weight (BW), adipose tissue, and serum leptin level.					
31636375	11	1	theme	mRNA	1524:1527	arg1	expressions					1529:1539	glutamate receptor mRNA expressions	1505:1539	glutamate receptor mRNA expressions	1505:1539	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	11	2	theme	glutamate	1505:1513	arg1	expressions					1529:1539	glutamate receptor mRNA expressions	1505:1539	glutamate receptor mRNA expressions	1505:1539	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	8	3	theme	serum	1161:1165	arg1	level					1174:1178	serum leptin level	1161:1178	serum leptin level	1161:1178	RESULTS Both high-fat/high-sucrose diets increased body weight (BW), adipose tissue, and serum leptin level.					
31636375	6	4	theme	mRNA	856:859	arg1	expression					842:851	The expression	838:851	The expression of mRNA related to neurotransmitter and nutrient transporters in the prefrontal cortex	838:938	The expression of mRNA related to neurotransmitter and nutrient transporters in the prefrontal cortex were determined using real-time PCR.					
31636375	11	5	theme	prefrontal	1548:1557	arg1	cortex					1559:1564	the prefrontal cortex	1544:1564	the prefrontal cortex	1544:1564	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	14	6	theme	high-fat	1903:1910	arg1	obesity					1925:1931	high-fat diet-induced obesity	1903:1931	high-fat diet-induced obesity	1903:1931	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	4	7	theme	diet	656:659	arg1	types					617:621	different types	607:621	different types of high-fat (lard and olive oil) diet with high sucrose	607:677	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	13	8	theme	anxiety-like	1749:1760	arg1	behavior					1762:1769	anxiety-like behavior	1749:1769	anxiety-like behavior	1749:1769	LPC conjugated with eicosapentaenoic acid (EPA) was strongly correlated with anxiety-like behavior.					
31636375	14	9	from	cause	1869:1873	arg1	obesity					1925:1931	high-fat diet-induced obesity	1903:1931	high-fat diet-induced obesity	1903:1931	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	14	10	theme	behavior	1891:1898	arg1	obesity					1842:1848	obesity	1842:1848	obesity per se	1842:1855	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	14	10	theme	behavior	1891:1898	arg1	composition					1817:1827	lipid composition	1811:1827	lipid composition	1811:1827	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	14	10	theme	behavior	1891:1898	arg1	cause					1869:1873	a major cause	1861:1873	a major cause of anxiety-like behavior in high-fat diet-induced obesity	1861:1931	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	12	11	theme	lysophosphatidyl	1582:1597	arg1	choline					1599:1605	serum lysophosphatidyl choline	1576:1605	serum lysophosphatidyl choline conjugated with several fatty acids	1576:1641	Further, serum lysophosphatidyl choline conjugated with several fatty acids was decreased by HL/HS diet.					
31636375	1	12	theme	risk	188:191	arg1	factor					193:198	a risk factor	186:198	a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression	186:308	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	1	12	theme	risk	188:191	arg1	Obesity					158:164	BACKGROUND Obesity	147:164	BACKGROUND Obesity	147:164	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	7	13	theme	mass	1053:1056	arg1	spectrometry					1058:1069	tandem mass spectrometry	1046:1069	tandem mass spectrometry	1046:1069	Serum lipid species were determined using liquid chromatography with tandem mass spectrometry.					
31636375	4	14	with	types	617:621	arg1	sucrose					671:677	high sucrose	666:677	high sucrose	666:677	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	14	15	theme	major	1863:1867	arg1	obesity					1842:1848	obesity	1842:1848	obesity per se	1842:1855	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	14	15	theme	major	1863:1867	arg1	composition					1817:1827	lipid composition	1811:1827	lipid composition	1811:1827	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	14	15	theme	major	1863:1867	arg1	cause					1869:1873	a major cause	1861:1873	a major cause of anxiety-like behavior in high-fat diet-induced obesity	1861:1931	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	8	16	dep	RESULTS	1072:1078	arg1	increased					1113:1121	increased	1113:1121	increased body weight (BW), adipose tissue, and serum leptin level	1113:1178	RESULTS Both high-fat/high-sucrose diets increased body weight (BW), adipose tissue, and serum leptin level.					
31636375	7	17	theme	lipid	983:987	arg1	species					989:995	Serum lipid species	977:995	Serum lipid species	977:995	Serum lipid species were determined using liquid chromatography with tandem mass spectrometry.					
31636375	7	18	theme	liquid	1019:1024	arg1	chromatography					1026:1039	liquid chromatography	1019:1039	liquid chromatography	1019:1039	Serum lipid species were determined using liquid chromatography with tandem mass spectrometry.					
31636375	4	19	theme	high	666:669	arg1	sucrose					671:677	high sucrose	666:677	high sucrose	666:677	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	0	20	theme	anxiety-like	45:56	arg1	behavior					58:65	anxiety-like behavior	45:65	anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level	45:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	13	21	theme	eicosapentaenoic	1692:1707	arg1	acid					1709:1712	eicosapentaenoic acid	1692:1712	eicosapentaenoic acid (EPA)	1692:1718	LPC conjugated with eicosapentaenoic acid (EPA) was strongly correlated with anxiety-like behavior.					
31636375	13	21	theme	eicosapentaenoic	1692:1707	arg1	EPA					1715:1717	EPA	1715:1717	EPA	1715:1717	LPC conjugated with eicosapentaenoic acid (EPA) was strongly correlated with anxiety-like behavior.					
31636375	5	22	theme	interaction	747:757	arg1	behavior					715:722	Anxiety-like behavior	702:722	Anxiety-like behavior (open-field and social interaction tests)	702:764	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	5	22	theme	interaction	747:757	arg1	tests					759:763	open-field and social interaction tests	725:763	open-field and social interaction tests	725:763	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	9	23	theme	anxiety-like	1266:1277	arg1	behavior					1279:1286	anxiety-like behavior	1266:1286	anxiety-like behavior	1266:1286	However, the high-lard/high-sucrose (HL/HS), but not high-olive oil/HS, diet induced anxiety-like behavior in open field and social interaction tests.					
31636375	10	24	theme	anxiety-like	1408:1419	arg1	behavior					1421:1428	anxiety-like behavior	1408:1428	anxiety-like behavior	1408:1428	BW and endocrine hormones such as leptin and insulin were not correlated to anxiety-like behavior.					
31636375	0	25	theme	obese	83:87	arg1	rats					89:92	diet-induced obese rats	70:92	diet-induced obese rats in relation to serum lysophosphatidyl choline level	70:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	4	26	dep	METHODS	561:567	arg1	treated					594:600	treated	594:600	were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks	589:699	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	6	27	theme	real-time	962:970	arg1	PCR					972:974	real-time PCR	962:974	real-time PCR	962:974	The expression of mRNA related to neurotransmitter and nutrient transporters in the prefrontal cortex were determined using real-time PCR.					
31636375	0	28	from	cause	36:40	arg1	rats					89:92	diet-induced obese rats	70:92	diet-induced obese rats in relation to serum lysophosphatidyl choline level	70:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	15	29	theme	Decreased	1934:1942	arg1	levels					1944:1949	Decreased levels	1934:1949	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex	1934:2041	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex might be involve in the pathophysiology of the behavioral change.					
31636375	0	30	theme	behavior	58:65	arg1	acid					16:19	Saturated fatty acid	0:19	Saturated fatty acid	0:19	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	0	30	theme	behavior	58:65	arg1	cause					36:40	a principal cause	24:40	a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level	24:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	2	31	theme	animal	370:375	arg1	experiments					377:387	model animal experiments	364:387	model animal experiments	364:387	A high-lard diet is widely used to induce obesity in model animal experiments, which also leads to anxiety-like and depression-like behaviors.					
31636375	0	32	theme	Saturated	0:8	arg1	acid					16:19	Saturated fatty acid	0:19	Saturated fatty acid	0:19	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	0	32	theme	Saturated	0:8	arg1	cause					36:40	a principal cause	24:40	a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level	24:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	9	33	theme	social	1306:1311	arg1	tests					1325:1329	social interaction tests	1306:1329	social interaction tests	1306:1329	However, the high-lard/high-sucrose (HL/HS), but not high-olive oil/HS, diet induced anxiety-like behavior in open field and social interaction tests.					
31636375	12	34	theme	fatty	1631:1635	arg1	acids					1637:1641	several fatty acids	1623:1641	several fatty acids	1623:1641	Further, serum lysophosphatidyl choline conjugated with several fatty acids was decreased by HL/HS diet.					
31636375	2	35	used	used	338:341	arg2	diet					323:326	A high-lard diet	311:326	A high-lard diet	311:326	A high-lard diet is widely used to induce obesity in model animal experiments, which also leads to anxiety-like and depression-like behaviors.					
31636375	14	36	theme	lipid	1811:1815	arg1	obesity					1842:1848	obesity	1842:1848	obesity per se	1842:1855	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	14	36	theme	lipid	1811:1815	arg1	composition					1817:1827	lipid composition	1811:1827	lipid composition	1811:1827	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	14	36	theme	lipid	1811:1815	arg1	cause					1869:1873	a major cause	1861:1873	a major cause of anxiety-like behavior in high-fat diet-induced obesity	1861:1931	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	15	37	theme	glutamate	2001:2009	arg1	system					2011:2016	altered glutamate system	1993:2016	altered glutamate system	1993:2016	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex might be involve in the pathophysiology of the behavioral change.					
31636375	8	38	theme	high-fat/high-sucrose	1085:1105	arg1	diets					1107:1111	Both high-fat/high-sucrose diets	1080:1111	Both high-fat/high-sucrose diets	1080:1111	RESULTS Both high-fat/high-sucrose diets increased body weight (BW), adipose tissue, and serum leptin level.					
31636375	3	39	theme	dietary	483:489	arg1	source					495:500	dietary fat source	483:500	dietary fat source	483:500	However, the contribution of dietary fat source to these abnormal behaviors in obesity is largely unknown.					
31636375	5	40	theme	cognitive	770:778	arg1	function					780:787	cognitive function	770:787	cognitive function (Y-maze test)	770:801	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	5	40	theme	cognitive	770:778	arg1	test					797:800	Y-maze test	790:800	Y-maze test	790:800	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	4	41	dep	high-fat	626:633	arg1	lard					636:639	lard	636:639	lard	636:639	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	4	41	dep	high-fat	626:633	arg1	oil					651:653	olive oil	645:653	olive oil	645:653	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	3	42	from	contribution	467:478	arg1	obesity					533:539	obesity	533:539	obesity	533:539	However, the contribution of dietary fat source to these abnormal behaviors in obesity is largely unknown.					
31636375	1	43	theme	neurodegenerative-	204:221	arg1	diseases					240:247	neurodegenerative- and psychiatric- diseases	204:247	neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression	204:308	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	1	43	theme	neurodegenerative-	204:221	arg1	schizophrenia					280:292	schizophrenia	280:292	schizophrenia	280:292	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	1	43	theme	neurodegenerative-	204:221	arg1	depression					299:308	depression	299:308	depression	299:308	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	1	43	theme	neurodegenerative-	204:221	arg1	disease					271:277	Alzheimer's disease	259:277	Alzheimer's disease	259:277	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	15	44	theme	change	2101:2106	arg1	pathophysiology					2067:2081	the pathophysiology	2063:2081	the pathophysiology of the behavioral change	2063:2106	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex might be involve in the pathophysiology of the behavioral change.					
31636375	10	45	theme	endocrine	1339:1347	arg1	leptin					1366:1371	leptin	1366:1371	leptin	1366:1371	BW and endocrine hormones such as leptin and insulin were not correlated to anxiety-like behavior.					
31636375	10	45	theme	endocrine	1339:1347	arg1	insulin					1377:1383	insulin	1377:1383	insulin	1377:1383	BW and endocrine hormones such as leptin and insulin were not correlated to anxiety-like behavior.					
31636375	10	45	theme	endocrine	1339:1347	arg1	hormones					1349:1356	endocrine hormones	1339:1356	endocrine hormones such as leptin and insulin	1339:1383	BW and endocrine hormones such as leptin and insulin were not correlated to anxiety-like behavior.					
31636375	1	46	theme	psychiatric-	227:238	arg1	diseases					240:247	neurodegenerative- and psychiatric- diseases	204:247	neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression	204:308	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	1	46	theme	psychiatric-	227:238	arg1	schizophrenia					280:292	schizophrenia	280:292	schizophrenia	280:292	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	1	46	theme	psychiatric-	227:238	arg1	depression					299:308	depression	299:308	depression	299:308	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	1	46	theme	psychiatric-	227:238	arg1	disease					271:277	Alzheimer's disease	259:277	Alzheimer's disease	259:277	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	6	47	theme	nutrient	893:900	arg1	transporters					902:913	neurotransmitter and nutrient transporters	872:913	transporters	902:913	The expression of mRNA related to neurotransmitter and nutrient transporters in the prefrontal cortex were determined using real-time PCR.					
31636375	5	48	theme	social	740:745	arg1	interaction					747:757	social interaction	740:757	social interaction	740:757	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	3	49	theme	source	495:500	arg1	unknown					552:558	unknown	552:558	unknown	552:558	However, the contribution of dietary fat source to these abnormal behaviors in obesity is largely unknown.					
31636375	3	49	theme	source	495:500	arg1	contribution					467:478	the contribution	463:478	the contribution of dietary fat source to these abnormal behaviors in obesity	463:539	However, the contribution of dietary fat source to these abnormal behaviors in obesity is largely unknown.					
31636375	6	50	theme	neurotransmitter	872:887	arg1	transporters					902:913	neurotransmitter and nutrient transporters	872:913	transporters	902:913	The expression of mRNA related to neurotransmitter and nutrient transporters in the prefrontal cortex were determined using real-time PCR.					
31636375	4	51	theme	Sprague-Dawley	569:582	arg1	rats					584:587	Sprague-Dawley rats	569:587	Sprague-Dawley rats	569:587	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	11	52	theme	expressions	1529:1539	arg1	increase					1453:1460	an increase	1450:1460	an increase in glutamate transporter	1450:1485	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	11	52	theme	expressions	1529:1539	arg1	decrease					1493:1500	a decrease	1491:1500	a decrease of glutamate receptor mRNA expressions	1491:1539	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	6	53	theme	related	861:867	arg1	mRNA					856:859	mRNA	856:859	mRNA related to neurotransmitter and nutrient transporters in the prefrontal cortex	856:938	The expression of mRNA related to neurotransmitter and nutrient transporters in the prefrontal cortex were determined using real-time PCR.					
31636375	11	54	theme	receptor	1515:1522	arg1	expressions					1529:1539	glutamate receptor mRNA expressions	1505:1539	glutamate receptor mRNA expressions	1505:1539	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	14	55	theme	diet-induced	1912:1923	arg1	obesity					1925:1931	high-fat diet-induced obesity	1903:1931	high-fat diet-induced obesity	1903:1931	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	4	56	theme	olive	645:649	arg1	oil					651:653	olive oil	645:653	olive oil	645:653	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	2	57	theme	high-lard	313:321	arg1	diet					323:326	A high-lard diet	311:326	A high-lard diet	311:326	A high-lard diet is widely used to induce obesity in model animal experiments, which also leads to anxiety-like and depression-like behaviors.					
31636375	15	58	theme	altered	1993:1999	arg1	system					2011:2016	altered glutamate system	1993:2016	altered glutamate system	1993:2016	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex might be involve in the pathophysiology of the behavioral change.					
31636375	8	59	theme	leptin	1167:1172	arg1	level					1174:1178	serum leptin level	1161:1178	serum leptin level	1161:1178	RESULTS Both high-fat/high-sucrose diets increased body weight (BW), adipose tissue, and serum leptin level.					
31636375	14	60	dep	CONCLUSIONS	1772:1782	arg1	suggest					1798:1804	suggest	1798:1804	suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity	1798:1931	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	0	61	theme	serum	109:113	arg1	choline					132:138	serum lysophosphatidyl choline	109:138	serum lysophosphatidyl choline level	109:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	14	62	theme	anxiety-like	1878:1889	arg1	behavior					1891:1898	anxiety-like behavior	1878:1898	anxiety-like behavior	1878:1898	CONCLUSIONS These results suggest that lipid composition, rather than obesity per se, is a major cause of anxiety-like behavior in high-fat diet-induced obesity.					
31636375	4	63	theme	high-fat	626:633	arg1	diet					656:659	high-fat (lard and olive oil) diet	626:659	high-fat (lard and olive oil) diet	626:659	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	15	64	theme	LPC	1965:1967	arg1	levels					1944:1949	Decreased levels	1934:1949	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex	1934:2041	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex might be involve in the pathophysiology of the behavioral change.					
31636375	3	65	theme	fat	491:493	arg1	source					495:500	dietary fat source	483:500	dietary fat source	483:500	However, the contribution of dietary fat source to these abnormal behaviors in obesity is largely unknown.					
31636375	7	66	theme	tandem	1046:1051	arg1	spectrometry					1058:1069	tandem mass spectrometry	1046:1069	tandem mass spectrometry	1046:1069	Serum lipid species were determined using liquid chromatography with tandem mass spectrometry.					
31636375	6	67	theme	prefrontal	922:931	arg1	cortex					933:938	the prefrontal cortex	918:938	the prefrontal cortex	918:938	The expression of mRNA related to neurotransmitter and nutrient transporters in the prefrontal cortex were determined using real-time PCR.					
31636375	0	68	from	behavior	58:65	arg1	rats					89:92	diet-induced obese rats	70:92	diet-induced obese rats in relation to serum lysophosphatidyl choline level	70:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	12	69	theme	serum	1576:1580	arg1	choline					1599:1605	serum lysophosphatidyl choline	1576:1605	serum lysophosphatidyl choline conjugated with several fatty acids	1576:1641	Further, serum lysophosphatidyl choline conjugated with several fatty acids was decreased by HL/HS diet.					
31636375	11	70	theme	glutamate	1465:1473	arg1	transporter					1475:1485	glutamate transporter	1465:1485	glutamate transporter	1465:1485	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	9	71	theme	high-olive	1234:1243	arg1	oil/HS					1245:1250	high-olive oil/HS	1234:1250	high-olive oil/HS	1234:1250	However, the high-lard/high-sucrose (HL/HS), but not high-olive oil/HS, diet induced anxiety-like behavior in open field and social interaction tests.					
31636375	0	72	from	rats	89:92	arg1	acid					16:19	Saturated fatty acid	0:19	Saturated fatty acid	0:19	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	0	72	from	rats	89:92	arg1	cause					36:40	a principal cause	24:40	a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level	24:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	0	72	from	rats	89:92	arg1	relation					97:104	relation	97:104	relation to serum lysophosphatidyl choline level	97:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	5	73	theme	open-field	725:734	arg1	behavior					715:722	Anxiety-like behavior	702:722	Anxiety-like behavior (open-field and social interaction tests)	702:764	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	5	73	theme	open-field	725:734	arg1	tests					759:763	open-field and social interaction tests	725:763	open-field and social interaction tests	725:763	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	7	74	theme	Serum	977:981	arg1	species					989:995	Serum lipid species	977:995	Serum lipid species	977:995	Serum lipid species were determined using liquid chromatography with tandem mass spectrometry.					
31636375	5	75	theme	Anxiety-like	702:713	arg1	behavior					715:722	Anxiety-like behavior	702:722	Anxiety-like behavior (open-field and social interaction tests)	702:764	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	5	75	theme	Anxiety-like	702:713	arg1	tests					759:763	open-field and social interaction tests	725:763	open-field and social interaction tests	725:763	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	0	76	theme	diet-induced	70:81	arg1	rats					89:92	diet-induced obese rats	70:92	diet-induced obese rats in relation to serum lysophosphatidyl choline level	70:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	1	77	theme	BACKGROUND	147:156	arg1	factor					193:198	a risk factor	186:198	a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression	186:308	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	1	77	theme	BACKGROUND	147:156	arg1	Obesity					158:164	BACKGROUND Obesity	147:164	BACKGROUND Obesity	147:164	BACKGROUND Obesity is considered to be a risk factor for neurodegenerative- and psychiatric- diseases including Alzheimer's disease, schizophrenia, and depression.					
31636375	15	78	theme	peripheral	1954:1963	arg1	LPC					1965:1967	peripheral LPC	1954:1967	peripheral LPC conjugated with EPA and altered glutamate system	1954:2016	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex might be involve in the pathophysiology of the behavioral change.					
31636375	9	79	theme	open	1291:1294	arg1	field					1296:1300	open field	1291:1300	open field	1291:1300	However, the high-lard/high-sucrose (HL/HS), but not high-olive oil/HS, diet induced anxiety-like behavior in open field and social interaction tests.					
31636375	11	80	from	decrease	1493:1500	arg1	transporter					1475:1485	glutamate transporter	1465:1485	glutamate transporter	1465:1485	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	0	81	theme	fatty	10:14	arg1	acid					16:19	Saturated fatty acid	0:19	Saturated fatty acid	0:19	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	0	81	theme	fatty	10:14	arg1	cause					36:40	a principal cause	24:40	a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level	24:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	15	82	from	levels	1944:1949	arg1	cortex					2036:2041	the prefrontal cortex	2021:2041	the prefrontal cortex	2021:2041	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex might be involve in the pathophysiology of the behavioral change.					
31636375	11	83	from	increase	1453:1460	arg1	transporter					1475:1485	glutamate transporter	1465:1485	glutamate transporter	1465:1485	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	12	84	theme	HL/HS	1660:1664	arg1	diet					1666:1669	HL/HS diet	1660:1669	HL/HS diet	1660:1669	Further, serum lysophosphatidyl choline conjugated with several fatty acids was decreased by HL/HS diet.					
31636375	2	85	theme	depression-like	427:441	arg1	behaviors					443:451	anxiety-like and depression-like behaviors	410:451	behaviors	443:451	A high-lard diet is widely used to induce obesity in model animal experiments, which also leads to anxiety-like and depression-like behaviors.					
31636375	2	86	theme	model	364:368	arg1	experiments					377:387	model animal experiments	364:387	model animal experiments	364:387	A high-lard diet is widely used to induce obesity in model animal experiments, which also leads to anxiety-like and depression-like behaviors.					
31636375	5	87	theme	Y-maze	790:795	arg1	function					780:787	cognitive function	770:787	cognitive function (Y-maze test)	770:801	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	5	87	theme	Y-maze	790:795	arg1	test					797:800	Y-maze test	790:800	Y-maze test	790:800	Anxiety-like behavior (open-field and social interaction tests) and cognitive function (Y-maze test) after the treatment were analyzed.					
31636375	9	88	theme	interaction	1313:1323	arg1	tests					1325:1329	social interaction tests	1306:1329	social interaction tests	1306:1329	However, the high-lard/high-sucrose (HL/HS), but not high-olive oil/HS, diet induced anxiety-like behavior in open field and social interaction tests.					
31636375	15	89	theme	prefrontal	2025:2034	arg1	cortex					2036:2041	the prefrontal cortex	2021:2041	the prefrontal cortex	2021:2041	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex might be involve in the pathophysiology of the behavioral change.					
31636375	12	90	theme	several	1623:1629	arg1	acids					1637:1641	several fatty acids	1623:1641	several fatty acids	1623:1641	Further, serum lysophosphatidyl choline conjugated with several fatty acids was decreased by HL/HS diet.					
31636375	2	91	theme	anxiety-like	410:421	arg1	behaviors					443:451	anxiety-like and depression-like behaviors	410:451	behaviors	443:451	A high-lard diet is widely used to induce obesity in model animal experiments, which also leads to anxiety-like and depression-like behaviors.					
31636375	11	92	theme	HL/HS	1431:1435	arg1	diet					1437:1440	HL/HS diet	1431:1440	HL/HS diet	1431:1440	HL/HS diet induced an increase in glutamate transporter and a decrease of glutamate receptor mRNA expressions in the prefrontal cortex.					
31636375	0	93	theme	principal	26:34	arg1	acid					16:19	Saturated fatty acid	0:19	Saturated fatty acid	0:19	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	0	93	theme	principal	26:34	arg1	cause					36:40	a principal cause	24:40	a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level	24:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	8	94	theme	body	1123:1126	arg1	BW					1136:1137	BW	1136:1137	BW	1136:1137	RESULTS Both high-fat/high-sucrose diets increased body weight (BW), adipose tissue, and serum leptin level.					
31636375	8	94	theme	body	1123:1126	arg1	weight					1128:1133	body weight	1123:1133	body weight (BW)	1123:1138	RESULTS Both high-fat/high-sucrose diets increased body weight (BW), adipose tissue, and serum leptin level.					
31636375	0	95	theme	lysophosphatidyl	115:130	arg1	choline					132:138	serum lysophosphatidyl choline	109:138	serum lysophosphatidyl choline level	109:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	6	96	from	transporters	902:913	arg1	cortex					933:938	the prefrontal cortex	918:938	the prefrontal cortex	918:938	The expression of mRNA related to neurotransmitter and nutrient transporters in the prefrontal cortex were determined using real-time PCR.					
31636375	0	97	theme	choline	132:138	arg1	level					140:144	serum lysophosphatidyl choline level	109:144	serum lysophosphatidyl choline level	109:144	Saturated fatty acid is a principal cause of anxiety-like behavior in diet-induced obese rats in relation to serum lysophosphatidyl choline level.					
31636375	15	98	theme	behavioral	2090:2099	arg1	change					2101:2106	the behavioral change	2086:2106	the behavioral change	2086:2106	Decreased levels of peripheral LPC conjugated with EPA and altered glutamate system in the prefrontal cortex might be involve in the pathophysiology of the behavioral change.					
31636375	4	99	theme	different	607:615	arg1	types					617:621	different types	607:621	different types of high-fat (lard and olive oil) diet with high sucrose	607:677	METHODS Sprague-Dawley rats were treated with different types of high-fat (lard and olive oil) diet with high sucrose for more than 8 weeks.					
31636375	3	100	theme	abnormal	511:518	arg1	behaviors					520:528	these abnormal behaviors	505:528	these abnormal behaviors	505:528	However, the contribution of dietary fat source to these abnormal behaviors in obesity is largely unknown.					
29361908	4	0	theme	potato	920:925	arg1	discoloration					978:990	enzymatic discoloration	968:990	enzymatic discoloration	968:990	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	0	theme	potato	920:925	arg1	traits					941:946	26 potato tuber quality traits	917:946	26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration)	917:991	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	12	1	theme	tuber	1939:1943	arg1	traits					1953:1958	the most interesting tuber quality traits	1918:1958	the most interesting tuber quality traits	1918:1958	Some of the co-localizing protein spots associated with some of the most interesting tuber quality traits were identified, albeit far less than we had anticipated at the onset of the experiments.					
29361908	4	2	theme	abundances	1073:1082	arg1	co-localization					996:1010	co-localization	996:1010	co-localization on the genetic map	996:1029	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	2	theme	abundances	1073:1082	arg1	study					1056:1060	a direct correlation study	1035:1060	a direct correlation study of protein abundances and phenotypic traits	1035:1104	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	9	3	theme	multiple	1546:1553	arg1	QTLs					1555:1558	multiple QTLs	1546:1558	multiple QTLs	1546:1558	For some 20 protein spots multiple QTLs were observed.					
29361908	3	4	theme	proteins	733:740	arg1	representation					705:718	a better representation	696:718	a better representation of the other proteins	696:740	Upon analyzing we made sure that the proteins from the patatin family were excluded to ensure a better representation of the other proteins.					
29361908	1	5	theme	quality	278:284	arg1	traits					286:291	quality traits	278:291	quality traits in non-model species	278:312	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	10	6	from	analysis	1597:1604	arg1	CONCLUSIONS					1575:1585	CONCLUSIONS	1575:1585	CONCLUSIONS From this analysis	1575:1604	CONCLUSIONS From this analysis, hotspot areas for protein QTLs were identified on chromosomes three, five, eight and nine.					
29361908	10	6	from	analysis	1597:1604	arg1	areas					1615:1619	hotspot areas	1607:1619	hotspot areas for protein QTLs	1607:1636	CONCLUSIONS From this analysis, hotspot areas for protein QTLs were identified on chromosomes three, five, eight and nine.					
29361908	3	7	from	family	665:670	arg1	proteins					639:646	the proteins	635:646	the proteins from the patatin family	635:670	Upon analyzing we made sure that the proteins from the patatin family were excluded to ensure a better representation of the other proteins.					
29361908	7	8	dep	evenly	1383:1388	arg1	not					1379:1381	not	1379:1381	not	1379:1381	pQTLs were observed on every chromosome although not evenly distributed over the chromosomes.					
29361908	2	9	theme	diploid	567:573	arg1	population					590:599	a diploid potato mapping population	565:599	a diploid potato mapping population	565:599	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	2	10	from	variation	371:379	arg1	traits					392:397	quality traits	384:397	quality traits related to carbohydrate and starch metabolism	384:443	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	4	11	theme	flesh	954:958	arg1	colour					960:965	flesh colour	954:965	flesh colour	954:965	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	2	12	from	content	492:498	arg1	tubers					526:531	potato tubers	519:531	potato tubers	519:531	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	6	13	theme	protein	1233:1239	arg1	spots					1241:1245	over 300 different protein spots	1214:1245	over 300 different protein spots some of which co-localized with traits such as starch content and cold sweetening	1214:1327	We were able to map pQTLs for over 300 different protein spots some of which co-localized with traits such as starch content and cold sweetening.					
29361908	12	14	theme	experiments	2037:2047	arg1	onset					2024:2028	the onset	2020:2028	the onset of the experiments	2020:2047	Some of the co-localizing protein spots associated with some of the most interesting tuber quality traits were identified, albeit far less than we had anticipated at the onset of the experiments.					
29361908	10	15	theme	hotspot	1607:1613	arg1	CONCLUSIONS					1575:1585	CONCLUSIONS	1575:1585	CONCLUSIONS From this analysis	1575:1604	CONCLUSIONS From this analysis, hotspot areas for protein QTLs were identified on chromosomes three, five, eight and nine.					
29361908	10	15	theme	hotspot	1607:1613	arg1	areas					1615:1619	hotspot areas	1607:1619	hotspot areas for protein QTLs	1607:1636	CONCLUSIONS From this analysis, hotspot areas for protein QTLs were identified on chromosomes three, five, eight and nine.					
29361908	4	16	dep	colour	960:965	arg1	e.g.					949:952	e.g.	949:952	e.g.	949:952	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	11	17	from	hotspot	1702:1708	arg1	chromosome					1713:1722	chromosome 3	1713:1724	chromosome 3	1713:1724	The hotspot on chromosome 3 coincided with a QTL previously identified for total protein content and had more than 23 pQTLs in the region from 70 to 80 cM.					
29361908	2	18	from	composition	504:514	arg1	tubers					526:531	potato tubers	519:531	potato tubers	519:531	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	2	19	theme	mapping	582:588	arg1	population					590:599	a diploid potato mapping population	565:599	a diploid potato mapping population	565:599	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	11	20	theme	protein	1779:1785	arg1	content					1787:1793	total protein content	1773:1793	total protein content	1773:1793	The hotspot on chromosome 3 coincided with a QTL previously identified for total protein content and had more than 23 pQTLs in the region from 70 to 80 cM.					
29361908	4	21	from	representation	832:845	arg1	population					854:863	the population	850:863	the population	850:863	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	22	theme	quality	933:939	arg1	discoloration					978:990	enzymatic discoloration	968:990	enzymatic discoloration	968:990	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	22	theme	quality	933:939	arg1	traits					941:946	26 potato tuber quality traits	917:946	26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration)	917:991	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	23	dep	RESULTS	743:749	arg1	established					869:879	established	869:879	established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits	869:1104	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	23	dep	RESULTS	743:749	arg1	performed					767:775	performed	767:775	performed pQTL analyses for all other proteins with a sufficient representation in the population	767:863	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	2	24	theme	potato	575:580	arg1	population					590:599	a diploid potato mapping population	565:599	a diploid potato mapping population	565:599	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	2	25	theme	2D-gel	539:544	arg1	electrophoresis					546:560	2D-gel electrophoresis	539:560	2D-gel electrophoresis	539:560	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	2	26	from	sweetening	454:463	arg1	traits					392:397	quality traits	384:397	quality traits related to carbohydrate and starch metabolism	384:443	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	5	27	theme	protein	1124:1130	arg1	spots					1132:1136	Over 1643 unique protein spots	1107:1136	Over 1643 unique protein spots	1107:1136	Over 1643 unique protein spots were detected in total over the two harvests.					
29361908	2	28	theme	protein	484:490	arg1	content					492:498	protein content	484:498	protein content	484:498	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	4	29	theme	direct	1037:1042	arg1	correlation					1044:1054	a direct correlation	1035:1054	a direct correlation study of protein abundances and phenotypic traits	1035:1104	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	10	30	theme	protein	1625:1631	arg1	QTLs					1633:1636	protein QTLs	1625:1636	protein QTLs	1625:1636	CONCLUSIONS From this analysis, hotspot areas for protein QTLs were identified on chromosomes three, five, eight and nine.					
29361908	0	31	theme	Genetical	0:8	arg1	genomics					10:17	Genetical genomics	0:17	Genetical genomics of quality related traits in potato tubers using proteomics.	0:78	Genetical genomics of quality related traits in potato tubers using proteomics.					
29361908	4	32	from	study	1056:1060	arg1	map					1027:1029	the genetic map	1015:1029	the genetic map	1015:1029	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	1	33	theme	Recent	91:96	arg1	advances					98:105	BACKGROUND Recent advances	80:105	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling	80:212	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	2	34	theme	cold	449:452	arg1	sweetening					454:463	cold sweetening	449:463	cold sweetening	449:463	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	1	35	theme	non-model	296:304	arg1	species					306:312	non-model species	296:312	non-model species	296:312	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	12	36	dep	had	2001:2003	arg1	anticipated					2005:2015	anticipated	2005:2015	had anticipated at the onset of the experiments	2001:2047	Some of the co-localizing protein spots associated with some of the most interesting tuber quality traits were identified, albeit far less than we had anticipated at the onset of the experiments.					
29361908	4	37	theme	genetic	1019:1025	arg1	map					1027:1029	the genetic map	1015:1029	the genetic map	1015:1029	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	0	38	theme	related	30:36	arg1	traits					38:43	quality related traits	22:43	quality related traits	22:43	Genetical genomics of quality related traits in potato tubers using proteomics.					
29361908	12	39	dep	albeit	1977:1982	arg1	less					1988:1991	less	1988:1991	less	1988:1991	Some of the co-localizing protein spots associated with some of the most interesting tuber quality traits were identified, albeit far less than we had anticipated at the onset of the experiments.					
29361908	2	40	theme	genetic	344:350	arg1	factors					352:358	the genetic factors	340:358	the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening	340:463	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	1	41	theme	~omics	110:115	arg1	proteomics					172:181	proteomics	172:181	proteomics	172:181	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	1	41	theme	~omics	110:115	arg1	metabolomics					155:166	metabolomics	155:166	metabolomics	155:166	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	1	41	theme	~omics	110:115	arg1	technologies					117:128	~omics technologies	110:128	~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling	110:212	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	1	41	theme	~omics	110:115	arg1	transcriptomics					138:152	transcriptomics	138:152	transcriptomics	138:152	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	2	42	dep	content	492:498	arg1	the					480:482	the	480:482	the	480:482	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	11	43	contain	had	1799:1801	arg1	hotspot					1702:1708	The hotspot	1698:1708	The hotspot on chromosome 3	1698:1724	The hotspot on chromosome 3 coincided with a QTL previously identified for total protein content and had more than 23 pQTLs in the region from 70 to 80 cM.					
29361908	11	43	contain	had	1799:1801	arg2	pQTLs					1816:1820	more than 23 pQTLs	1803:1820	more than 23 pQTLs	1803:1820	The hotspot on chromosome 3 coincided with a QTL previously identified for total protein content and had more than 23 pQTLs in the region from 70 to 80 cM.					
29361908	4	44	theme	traits	1099:1104	arg1	co-localization					996:1010	co-localization	996:1010	co-localization on the genetic map	996:1029	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	44	theme	traits	1099:1104	arg1	study					1056:1060	a direct correlation study	1035:1060	a direct correlation study of protein abundances and phenotypic traits	1035:1104	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	1	45	theme	genetic	233:239	arg1	dissection					241:250	the genetic dissection	229:250	the genetic dissection of complex traits such as quality traits in non-model species	229:312	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	7	46	located	observed	1341:1348	arg2	pQTLs					1330:1334	pQTLs	1330:1334	pQTLs	1330:1334	pQTLs were observed on every chromosome although not evenly distributed over the chromosomes.					
29361908	7	46	located	observed	1341:1348	arg1	chromosome					1359:1368	every chromosome	1353:1368	every chromosome although not evenly distributed over the chromosomes	1353:1421	pQTLs were observed on every chromosome although not evenly distributed over the chromosomes.					
29361908	4	47	theme	protein	1065:1071	arg1	abundances					1073:1082	protein abundances	1065:1082	protein abundances	1065:1082	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	1	48	theme	traits	263:268	arg1	dissection					241:250	the genetic dissection	229:250	the genetic dissection of complex traits such as quality traits in non-model species	229:312	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	3	49	theme	better	698:703	arg1	representation					705:718	a better representation	696:718	a better representation of the other proteins	696:740	Upon analyzing we made sure that the proteins from the patatin family were excluded to ensure a better representation of the other proteins.					
29361908	12	50	theme	interesting	1927:1937	arg1	traits					1953:1958	the most interesting tuber quality traits	1918:1958	the most interesting tuber quality traits	1918:1958	Some of the co-localizing protein spots associated with some of the most interesting tuber quality traits were identified, albeit far less than we had anticipated at the onset of the experiments.					
29361908	12	51	theme	quality	1945:1951	arg1	traits					1953:1958	the most interesting tuber quality traits	1918:1958	the most interesting tuber quality traits	1918:1958	Some of the co-localizing protein spots associated with some of the most interesting tuber quality traits were identified, albeit far less than we had anticipated at the onset of the experiments.					
29361908	3	52	theme	other	727:731	arg1	proteins					733:740	the other proteins	723:740	the other proteins	723:740	Upon analyzing we made sure that the proteins from the patatin family were excluded to ensure a better representation of the other proteins.					
29361908	1	53	from	traits	286:291	arg1	species					306:312	non-model species	296:312	non-model species	296:312	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	2	54	theme	more	322:325	arg1	insight					327:333	more insight	322:333	more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening	322:463	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	8	55	theme	chromosome	1498:1507	arg1	number					1509:1514	chromosome number 10	1498:1517	chromosome number 10	1498:1517	The largest number of pQTLs was found for chromosome 8 and the lowest for chromosome number 10.					
29361908	11	56	theme	total	1773:1777	arg1	content					1787:1793	total protein content	1773:1793	total protein content	1773:1793	The hotspot on chromosome 3 coincided with a QTL previously identified for total protein content and had more than 23 pQTLs in the region from 70 to 80 cM.					
29361908	8	57	theme	pQTLs	1446:1450	arg1	number					1436:1441	The largest number	1424:1441	The largest number of pQTLs	1424:1450	The largest number of pQTLs was found for chromosome 8 and the lowest for chromosome number 10.					
29361908	1	58	theme	genotypic	194:202	arg1	profiling					204:212	genotypic profiling	194:212	genotypic profiling	194:212	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	12	59	theme	protein	1880:1886	arg1	spots					1888:1892	the co-localizing protein spots	1862:1892	the co-localizing protein spots associated with some of the most interesting tuber quality traits	1862:1958	Some of the co-localizing protein spots associated with some of the most interesting tuber quality traits were identified, albeit far less than we had anticipated at the onset of the experiments.					
29361908	6	60	theme	different	1223:1231	arg1	spots					1241:1245	over 300 different protein spots	1214:1245	over 300 different protein spots some of which co-localized with traits such as starch content and cold sweetening	1214:1327	We were able to map pQTLs for over 300 different protein spots some of which co-localized with traits such as starch content and cold sweetening.					
29361908	5	61	located	detected	1143:1150	arg2	spots					1132:1136	Over 1643 unique protein spots	1107:1136	Over 1643 unique protein spots	1107:1136	Over 1643 unique protein spots were detected in total over the two harvests.					
29361908	5	61	located	detected	1143:1150	arg1	total					1155:1159	total	1155:1159	total	1155:1159	Over 1643 unique protein spots were detected in total over the two harvests.					
29361908	6	62	theme	starch	1294:1299	arg1	content					1301:1307	starch content	1294:1307	starch content	1294:1307	We were able to map pQTLs for over 300 different protein spots some of which co-localized with traits such as starch content and cold sweetening.					
29361908	4	63	theme	tuber	927:931	arg1	discoloration					978:990	enzymatic discoloration	968:990	enzymatic discoloration	968:990	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	63	theme	tuber	927:931	arg1	traits					941:946	26 potato tuber quality traits	917:946	26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration)	917:991	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	11	64	from	80 cM	1847:1851	arg1	region					1829:1834	the region	1825:1834	the region from 70 to 80 cM	1825:1851	The hotspot on chromosome 3 coincided with a QTL previously identified for total protein content and had more than 23 pQTLs in the region from 70 to 80 cM.					
29361908	0	65	from	genomics	10:17	arg1	tubers					55:60	potato tubers	48:60	potato tubers using proteomics	48:77	Genetical genomics of quality related traits in potato tubers using proteomics.					
29361908	12	66	theme	co-localizing	1866:1878	arg1	spots					1888:1892	the co-localizing protein spots	1862:1892	the co-localizing protein spots associated with some of the most interesting tuber quality traits	1862:1958	Some of the co-localizing protein spots associated with some of the most interesting tuber quality traits were identified, albeit far less than we had anticipated at the onset of the experiments.					
29361908	4	67	theme	pQTL	777:780	arg1	analyses					782:789	pQTL analyses	777:789	pQTL analyses for all other proteins	777:812	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	5	68	theme	unique	1117:1122	arg1	spots					1132:1136	Over 1643 unique protein spots	1107:1136	Over 1643 unique protein spots	1107:1136	Over 1643 unique protein spots were detected in total over the two harvests.					
29361908	9	69	theme	protein	1532:1538	arg1	spots					1540:1544	some 20 protein spots	1524:1544	some 20 protein spots	1524:1544	For some 20 protein spots multiple QTLs were observed.					
29361908	2	70	theme	potato	519:524	arg1	tubers					526:531	potato tubers	519:531	potato tubers	519:531	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	2	71	theme	related	399:405	arg1	traits					392:397	quality traits	384:397	quality traits related to carbohydrate and starch metabolism	384:443	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	1	72	theme	BACKGROUND	80:89	arg1	advances					98:105	BACKGROUND Recent advances	80:105	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling	80:212	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	2	73	theme	quality	384:390	arg1	traits					392:397	quality traits	384:397	quality traits related to carbohydrate and starch metabolism	384:443	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	0	74	theme	quality	22:28	arg1	traits					38:43	quality related traits	22:43	quality related traits	22:43	Genetical genomics of quality related traits in potato tubers using proteomics.					
29361908	4	75	theme	correlation	1044:1054	arg1	study					1056:1060	a direct correlation study	1035:1060	a direct correlation study of protein abundances and phenotypic traits	1035:1104	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	3	76	theme	patatin	657:663	arg1	family					665:670	the patatin family	653:670	the patatin family	653:670	Upon analyzing we made sure that the proteins from the patatin family were excluded to ensure a better representation of the other proteins.					
29361908	2	77	theme	starch	427:432	arg1	metabolism					434:443	starch metabolism	427:443	starch metabolism	427:443	To get more insight into the genetic factors underlying variation in quality traits related to carbohydrate and starch metabolism and cold sweetening, we determined the protein content and composition in potato tubers using 2D-gel electrophoresis in a diploid potato mapping population.					
29361908	0	78	theme	traits	38:43	arg1	genomics					10:17	Genetical genomics	0:17	Genetical genomics of quality related traits in potato tubers using proteomics.	0:78	Genetical genomics of quality related traits in potato tubers using proteomics.					
29361908	4	79	theme	sufficient	821:830	arg1	representation					832:845	a sufficient representation	819:845	a sufficient representation in the population	819:863	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	80	theme	enzymatic	968:976	arg1	discoloration					978:990	enzymatic discoloration	968:990	enzymatic discoloration	968:990	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	80	theme	enzymatic	968:976	arg1	traits					941:946	26 potato tuber quality traits	917:946	26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration)	917:991	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	0	81	theme	potato	48:53	arg1	tubers					55:60	potato tubers	48:60	potato tubers using proteomics	48:77	Genetical genomics of quality related traits in potato tubers using proteomics.					
29361908	11	82	dep	80 cM	1847:1851	arg1	to					1844:1845	to	1844:1845	to	1844:1845	The hotspot on chromosome 3 coincided with a QTL previously identified for total protein content and had more than 23 pQTLs in the region from 70 to 80 cM.					
29361908	6	83	theme	cold	1313:1316	arg1	sweetening					1318:1327	cold sweetening	1313:1327	cold sweetening	1313:1327	We were able to map pQTLs for over 300 different protein spots some of which co-localized with traits such as starch content and cold sweetening.					
29361908	1	84	from	advances	98:105	arg1	proteomics					172:181	proteomics	172:181	proteomics	172:181	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	1	84	from	advances	98:105	arg1	metabolomics					155:166	metabolomics	155:166	metabolomics	155:166	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	1	84	from	advances	98:105	arg1	technologies					117:128	~omics technologies	110:128	~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling	110:212	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	1	84	from	advances	98:105	arg1	transcriptomics					138:152	transcriptomics	138:152	transcriptomics	138:152	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	4	85	theme	phenotypic	1088:1097	arg1	traits					1099:1104	phenotypic traits	1088:1104	phenotypic traits	1088:1104	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	86	theme	other	799:803	arg1	proteins					805:812	all other proteins	795:812	all other proteins	795:812	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	4	87	from	co-localization	996:1010	arg1	map					1027:1029	the genetic map	1015:1029	the genetic map	1015:1029	RESULTS We subsequently performed pQTL analyses for all other proteins with a sufficient representation in the population and established a relationship between proteins and 26 potato tuber quality traits (e.g. flesh colour, enzymatic discoloration) by co-localization on the genetic map and a direct correlation study of protein abundances and phenotypic traits.					
29361908	8	88	theme	largest	1428:1434	arg1	number					1436:1441	The largest number	1424:1441	The largest number of pQTLs	1424:1450	The largest number of pQTLs was found for chromosome 8 and the lowest for chromosome number 10.					
29361908	1	89	theme	complex	255:261	arg1	traits					286:291	quality traits	278:291	quality traits in non-model species	278:312	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
29361908	1	89	theme	complex	255:261	arg1	traits					263:268	complex traits	255:268	complex traits such as quality traits in non-model species	255:312	BACKGROUND Recent advances in ~omics technologies such as transcriptomics, metabolomics and proteomics along with genotypic profiling have permitted the genetic dissection of complex traits such as quality traits in non-model species.					
31739640	0	0	theme	Microbiota	87:96	arg1	Composition					68:78	the Composition	64:78	the Composition of Gut Microbiota	64:96	The Constipation-Relieving Property of d-Tagatose by Modulating the Composition of Gut Microbiota.					
31739640	1	1	theme	related	304:310	arg1	mechanisms					312:321	related mechanisms	304:321	related mechanisms	304:321	d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear.					
31739640	5	2	theme	oxide	1075:1079	arg1	levels					1058:1063	the serum levels	1048:1063	the serum levels of nitric oxide (NO)	1048:1084	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	4	3	from	weight	842:847	arg1	hours					856:860	six hours	852:860	six hours	852:860	The results showed that the gastrointestinal transit rate, fecal number, and weight in six hours were significantly enhanced after the administration of d-tagatose.					
31739640	1	4	theme	food	226:229	arg1	industry					231:238	the food industry	222:238	the food industry	222:238	d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear.					
31739640	0	5	theme	Gut	83:85	arg1	Microbiota					87:96	Gut Microbiota	83:96	Gut Microbiota	83:96	The Constipation-Relieving Property of d-Tagatose by Modulating the Composition of Gut Microbiota.					
31739640	2	6	theme	d-tagatose	448:457	arg1	effects					437:443	the effects	433:443	the effects of d-tagatose for the prevention of constipation	433:492	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	6	7	theme	rRNA	1134:1137	arg1	analysis					1150:1157	the 16S rRNA sequencing analysis	1126:1157	the 16S rRNA sequencing analysis	1126:1157	Moreover, the 16S rRNA sequencing analysis revealed that the changes in the gut microbiota caused by constipation were restored by d-tagatose treatment.					
31739640	7	8	from	constipation	1403:1414	arg1	mice					1427:1430	Kunming mice	1419:1430	Kunming mice	1419:1430	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	2	9	theme	constipation	481:492	arg1	prevention					467:476	the prevention	463:476	the prevention of constipation	463:492	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	3	10	theme	gut	717:719	arg1	microbiota					721:730	gut microbiota	717:730	gut microbiota	717:730	Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups.					
31739640	7	11	with	candidate	1465:1473	arg1	properties					1503:1512	constipation-relieving properties	1480:1512	constipation-relieving properties	1480:1512	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	6	12	theme	16S	1130:1132	arg1	analysis					1150:1157	the 16S rRNA sequencing analysis	1126:1157	the 16S rRNA sequencing analysis	1126:1157	Moreover, the 16S rRNA sequencing analysis revealed that the changes in the gut microbiota caused by constipation were restored by d-tagatose treatment.					
31739640	5	13	dep	increased	968:976	arg1	whereas					1040:1046	whereas	1040:1046	whereas	1040:1046	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	3	14	theme	microbiota	721:730	arg1	amplicon					705:712	16S rRNA amplicon	696:712	16S rRNA amplicon of gut microbiota among the different mice groups	696:762	Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups.					
31739640	5	15	theme	nitric	1068:1073	arg1	NO					1082:1083	NO	1082:1083	NO	1082:1083	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	5	15	theme	nitric	1068:1073	arg1	oxide					1075:1079	nitric oxide	1068:1079	nitric oxide (NO)	1068:1084	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	4	16	theme	gastrointestinal	793:808	arg1	rate					818:821	the gastrointestinal transit rate	789:821	the gastrointestinal transit rate	789:821	The results showed that the gastrointestinal transit rate, fecal number, and weight in six hours were significantly enhanced after the administration of d-tagatose.					
31739640	5	17	theme	serum	982:986	arg1	levels					988:993	the serum levels	978:993	the serum levels of acetylcholine (Ach) and substance P (SP)	978:1037	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	7	18	theme	Kunming	1419:1425	arg1	mice					1427:1430	Kunming mice	1419:1430	Kunming mice	1419:1430	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	0	19	theme	Constipation-Relieving	4:25	arg1	Property					27:34	The Constipation-Relieving Property	0:34	The Constipation-Relieving Property of d-Tagatose	0:48	The Constipation-Relieving Property of d-Tagatose by Modulating the Composition of Gut Microbiota.					
31739640	4	20	from	number	830:835	arg1	hours					856:860	six hours	852:860	six hours	852:860	The results showed that the gastrointestinal transit rate, fecal number, and weight in six hours were significantly enhanced after the administration of d-tagatose.					
31739640	5	21	theme	acetylcholine	998:1010	arg1	levels					988:993	the serum levels	978:993	the serum levels of acetylcholine (Ach) and substance P (SP)	978:1037	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	3	22	theme	different	742:750	arg1	groups					757:762	the different mice groups	738:762	the different mice groups	738:762	Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups.					
31739640	6	23	theme	gut	1192:1194	arg1	microbiota					1196:1205	the gut microbiota	1188:1205	the gut microbiota	1188:1205	Moreover, the 16S rRNA sequencing analysis revealed that the changes in the gut microbiota caused by constipation were restored by d-tagatose treatment.					
31739640	5	24	theme	P	1032:1032	arg1	levels					988:993	the serum levels	978:993	the serum levels of acetylcholine (Ach) and substance P (SP)	978:1037	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	3	25	theme	mice	752:755	arg1	groups					757:762	the different mice groups	738:762	the different mice groups	738:762	Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups.					
31739640	2	26	theme	transit	529:535	arg1	experiment					537:546	gastrointestinal transit experiment	512:546	gastrointestinal transit experiment	512:546	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	7	27	theme	d-tagatose	1332:1341	arg1	administration					1314:1327	the administration	1310:1327	the administration of d-tagatose as a dietary supplement	1310:1365	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	7	28	theme	dietary	1348:1354	arg1	supplement					1356:1365	a dietary supplement	1346:1365	a dietary supplement	1346:1365	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	2	29	theme	Kunming	399:405	arg1	mice					407:410	the loperamide-induced constipation Kunming mice	363:410	the loperamide-induced constipation Kunming mice	363:410	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	2	29	theme	Kunming	399:405	arg1	model					426:430	the animal model	415:430	the animal model	415:430	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	1	30	theme	prebiotic	254:262	arg1	unclear					333:339	unclear	333:339	unclear	333:339	d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear.					
31739640	1	30	theme	prebiotic	254:262	arg1	activity					264:271	the prebiotic activity	250:271	the prebiotic activity	250:271	d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear.					
31739640	2	31	theme	gastrointestinal	512:527	arg1	experiment					537:546	gastrointestinal transit experiment	512:546	gastrointestinal transit experiment	512:546	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	2	32	theme	constipation	386:397	arg1	mice					407:410	the loperamide-induced constipation Kunming mice	363:410	the loperamide-induced constipation Kunming mice	363:410	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	2	32	theme	constipation	386:397	arg1	model					426:430	the animal model	415:430	the animal model	415:430	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	1	33	theme	wide	191:194	arg1	range					196:200	a wide range	189:200	a wide range of applicability	189:217	d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear.					
31739640	2	34	theme	loperamide-induced	367:384	arg1	mice					407:410	the loperamide-induced constipation Kunming mice	363:410	the loperamide-induced constipation Kunming mice	363:410	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	2	34	theme	loperamide-induced	367:384	arg1	model					426:430	the animal model	415:430	the animal model	415:430	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	0	35	theme	d-Tagatose	39:48	arg1	Property					27:34	The Constipation-Relieving Property	0:34	The Constipation-Relieving Property of d-Tagatose	0:48	The Constipation-Relieving Property of d-Tagatose by Modulating the Composition of Gut Microbiota.					
31739640	4	36	theme	d-tagatose	918:927	arg1	administration					900:913	the administration	896:913	the administration of d-tagatose	896:927	The results showed that the gastrointestinal transit rate, fecal number, and weight in six hours were significantly enhanced after the administration of d-tagatose.					
31739640	7	37	theme	constipation-relieving	1480:1501	arg1	properties					1503:1512	constipation-relieving properties	1480:1512	constipation-relieving properties	1480:1512	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	4	38	theme	transit	810:816	arg1	rate					818:821	the gastrointestinal transit rate	789:821	the gastrointestinal transit rate	789:821	The results showed that the gastrointestinal transit rate, fecal number, and weight in six hours were significantly enhanced after the administration of d-tagatose.					
31739640	1	39	theme	anticonstipation	274:289	arg1	effects					291:297	anticonstipation effects	274:297	anticonstipation effects	274:297	d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear.					
31739640	5	40	theme	serum	1052:1056	arg1	levels					1058:1063	the serum levels	1048:1063	the serum levels of nitric oxide (NO)	1048:1084	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	4	41	theme	fecal	824:828	arg1	number					830:835	fecal number	824:835	fecal number	824:835	The results showed that the gastrointestinal transit rate, fecal number, and weight in six hours were significantly enhanced after the administration of d-tagatose.					
31739640	2	42	theme	defecation	552:561	arg1	experiment					563:572	defecation experiment	552:572	defecation experiment	552:572	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	3	43	theme	biochemical	659:669	arg1	indicators					671:680	the biochemical indicators	655:680	the biochemical indicators	655:680	Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups.					
31739640	5	44	theme	substance	1022:1030	arg1	SP					1035:1036	SP	1035:1036	SP	1035:1036	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	5	44	theme	substance	1022:1030	arg1	P					1032:1032	substance P	1022:1032	substance P (SP)	1022:1037	In addition, d-tagatose significantly increased the serum levels of acetylcholine (Ach) and substance P (SP), whereas the serum levels of nitric oxide (NO) were significantly decreased.					
31739640	4	45	from	rate	818:821	arg1	hours					856:860	six hours	852:860	six hours	852:860	The results showed that the gastrointestinal transit rate, fecal number, and weight in six hours were significantly enhanced after the administration of d-tagatose.					
31739640	6	46	from	changes	1177:1183	arg1	microbiota					1196:1205	the gut microbiota	1188:1205	the gut microbiota	1188:1205	Moreover, the 16S rRNA sequencing analysis revealed that the changes in the gut microbiota caused by constipation were restored by d-tagatose treatment.					
31739640	3	47	theme	indicators	671:680	arg1	change					645:650	the change	641:650	the change of the biochemical indicators	641:680	Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups.					
31739640	6	48	theme	d-tagatose	1247:1256	arg1	treatment					1258:1266	d-tagatose treatment	1247:1266	d-tagatose treatment	1247:1266	Moreover, the 16S rRNA sequencing analysis revealed that the changes in the gut microbiota caused by constipation were restored by d-tagatose treatment.					
31739640	7	49	theme	promising	1445:1453	arg1	candidate					1465:1473	a promising prebiotic candidate	1443:1473	a promising prebiotic candidate with constipation-relieving properties	1443:1512	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	7	49	theme	promising	1445:1453	arg1	it					1437:1438	it	1437:1438	it	1437:1438	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	1	50	theme	dietary	141:147	arg1	d-tagatose					99:108	d-tagatose	99:108	d-tagatose	99:108	d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear.					
31739640	1	50	theme	dietary	141:147	arg1	supplement					149:158	a dietary supplement	139:158	a monosaccharide as well as a dietary supplement	111:158	d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear.					
31739640	1	51	theme	applicability	205:217	arg1	range					196:200	a wide range	189:200	a wide range of applicability	189:217	d-tagatose, a monosaccharide as well as a dietary supplement, has been reported as having a wide range of applicability in the food industry, however, the prebiotic activity, anticonstipation effects, and related mechanisms are still unclear.					
31739640	7	52	theme	prebiotic	1455:1463	arg1	candidate					1465:1473	a promising prebiotic candidate	1443:1473	a promising prebiotic candidate with constipation-relieving properties	1443:1512	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	7	52	theme	prebiotic	1455:1463	arg1	it					1437:1438	it	1437:1438	it	1437:1438	In conclusion, this study indicated that the administration of d-tagatose as a dietary supplement can effectively prevent and relieve constipation in Kunming mice, and it is a promising prebiotic candidate with constipation-relieving properties.					
31739640	3	53	theme	underlying	592:601	arg1	mechanism					603:611	the underlying mechanism	588:611	the underlying mechanism	588:611	Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups.					
31739640	3	54	theme	16S	696:698	arg1	rRNA					700:703	16S rRNA	696:703	16S rRNA amplicon of gut microbiota among the different mice groups	696:762	Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups.					
31739640	6	55	theme	sequencing	1139:1148	arg1	analysis					1150:1157	the 16S rRNA sequencing analysis	1126:1157	the 16S rRNA sequencing analysis	1126:1157	Moreover, the 16S rRNA sequencing analysis revealed that the changes in the gut microbiota caused by constipation were restored by d-tagatose treatment.					
31739640	3	56	theme	rRNA	700:703	arg1	amplicon					705:712	16S rRNA amplicon	696:712	16S rRNA amplicon of gut microbiota among the different mice groups	696:762	Furthermore, the underlying mechanism was clarified by evaluating the change of the biochemical indicators and analyzing 16S rRNA amplicon of gut microbiota among the different mice groups.					
31739640	2	57	theme	animal	419:424	arg1	mice					407:410	the loperamide-induced constipation Kunming mice	363:410	the loperamide-induced constipation Kunming mice	363:410	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31739640	2	57	theme	animal	419:424	arg1	model					426:430	the animal model	415:430	the animal model	415:430	In this study, using the loperamide-induced constipation Kunming mice as the animal model, the effects of d-tagatose for the prevention of constipation were evaluated by gastrointestinal transit experiment and defecation experiment.					
31540385	6	0	theme	Pancreatic	773:782	arg1	activity					792:799	Pancreatic amylase activity	773:799	Pancreatic amylase activity	773:799	Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus.					
31540385	0	1	dep	Male	109:112	arg1	in					106:107	in	106:107	in	106:107	A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice.					
31540385	7	2	theme	basal	958:962	arg1	EE					964:965	basal EE	958:965	basal EE	958:965	For both sexes, BW, BC, and basal EE and RER were not affected by the type of starch, but dEI and hydrogen production were increased by the LDD.					
31540385	1	3	theme	low	133:135	arg1	digestibility					137:149	low digestibility	133:149	low digestibility	133:149	Starches of low digestibility are associated with improved glucose metabolism.					
31540385	8	4	theme	hepatic	1289:1295	arg1	partitioning					1297:1308	hepatic partitioning	1289:1308	hepatic partitioning of the 13C label	1289:1325	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	7	5	theme	starch	1008:1013	arg1	type					1000:1003	the type	996:1003	the type of starch	996:1013	For both sexes, BW, BC, and basal EE and RER were not affected by the type of starch, but dEI and hydrogen production were increased by the LDD.					
31540385	4	6	theme	Body	440:443	arg1	BW					453:454	BW	453:454	BW	453:454	Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly.					
31540385	4	6	theme	Body	440:443	arg1	weight					445:450	Body weight	440:450	Body weight (BW)	440:455	Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly.					
31540385	8	7	theme	starch	1177:1182	arg1	bolus					1184:1188	the starch bolus	1173:1188	the starch bolus	1173:1188	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	4	8	theme	digestible	485:494	arg1	intake					503:508	digestible energy intake	485:508	digestible energy intake (dEI)	485:514	Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly.					
31540385	4	8	theme	digestible	485:494	arg1	dEI					511:513	dEI	511:513	dEI	511:513	Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly.					
31540385	0	9	theme	Female	90:95	arg1	Mice					115:118	Female, but Not in Male, Mice	90:118	Female, but Not in Male, Mice	90:118	A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice.					
31540385	8	10	link	starch-derived	1125:1138	arg1	oxidation					1148:1156	starch-derived glucose oxidation	1125:1156	starch-derived glucose oxidation	1125:1156	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	8	11	theme	glucose	1140:1146	arg1	oxidation					1148:1156	starch-derived glucose oxidation	1125:1156	starch-derived glucose oxidation	1125:1156	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	5	12	theme	extended	742:749	arg1	calorimetry					760:770	extended indirect calorimetry	742:770	extended indirect calorimetry	742:770	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	8	13	from	differences	1254:1264	arg1	activity					1277:1284	amylase activity	1269:1284	amylase activity	1269:1284	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	8	13	from	differences	1254:1264	arg1	partitioning					1297:1308	hepatic partitioning	1289:1308	hepatic partitioning of the 13C label	1289:1325	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	8	14	from	oxidation	1148:1156	arg1	response					1161:1168	response	1161:1168	response to the starch bolus	1161:1188	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	1	15	theme	digestibility	137:149	arg1	Starches					121:128	Starches	121:128	Starches of low digestibility	121:149	Starches of low digestibility are associated with improved glucose metabolism.					
31540385	8	16	dep	13C	1317:1319	arg1	label					1321:1325	label	1321:1325	label	1321:1325	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	5	17	theme	starch	712:717	arg1	bolus					719:723	an oral 13C-labelled starch bolus	691:723	an oral 13C-labelled starch bolus	691:723	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	0	18	theme	Lowly	2:6	arg1	Diet					26:29	A Lowly Digestible-Starch Diet	0:29	A Lowly Digestible-Starch Diet after Weaning	0:43	A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice.					
31540385	8	19	theme	13C	1317:1319	arg1	activity					1277:1284	amylase activity	1269:1284	amylase activity	1269:1284	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	8	19	theme	13C	1317:1319	arg1	partitioning					1297:1308	hepatic partitioning	1289:1308	hepatic partitioning of the 13C label	1289:1325	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	5	20	theme	respiratory	615:625	arg1	RER					643:645	RER	643:645	RER	643:645	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	20	theme	respiratory	615:625	arg1	ratio					636:640	respiratory exchange ratio	615:640	respiratory exchange ratio (RER)	615:646	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	21	theme	bolus	719:723	arg1	oxidation					678:686	the oxidation	674:686	the oxidation of an oral 13C-labelled starch bolus	674:723	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	21	theme	bolus	719:723	arg1	expenditure					597:607	whole-body energy expenditure	579:607	whole-body energy expenditure (EE)	579:612	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	21	theme	bolus	719:723	arg1	ratio					636:640	respiratory exchange ratio	615:640	respiratory exchange ratio (RER)	615:646	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	21	theme	bolus	719:723	arg1	production					658:667	hydrogen production	649:667	hydrogen production	649:667	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	21	theme	bolus	719:723	arg1	EE					610:611	EE	610:611	EE	610:611	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	21	theme	bolus	719:723	arg1	RER					643:645	RER	643:645	RER	643:645	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	8	22	from	oxidation	1111:1119	arg1	response					1161:1168	response	1161:1168	response to the starch bolus	1161:1188	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	9	23	theme	starch	1352:1357	arg1	digestibility					1359:1371	starch digestibility	1352:1371	starch digestibility	1352:1371	These results show that starch digestibility impacts glucose metabolism differently in females versus males.					
31540385	6	24	theme	bolus	923:927	arg1	administration					894:907	administration	894:907	administration of the starch bolus	894:927	Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus.					
31540385	4	25	theme	body	458:461	arg1	composition					463:473	body composition	458:473	body composition (BC)	458:478	Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly.					
31540385	4	25	theme	body	458:461	arg1	BC					476:477	BC	476:477	BC	476:477	Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly.					
31540385	0	26	theme	Male	109:112	arg1	Mice					115:118	Female, but Not in Male, Mice	90:118	Female, but Not in Male, Mice	90:118	A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice.					
31540385	2	27	theme	digestible-starch	273:289	arg1	HDD					297:299	HDD	297:299	HDD	297:299	We hypothesise that a lowly digestible-starch diet (LDD) versus a highly digestible-starch diet (HDD) improves the capacity to oxidise starch, and that this is sex-dependent.					
31540385	2	27	theme	digestible-starch	273:289	arg1	diet					291:294	a highly digestible-starch diet	264:294	a highly digestible-starch diet (HDD)	264:300	We hypothesise that a lowly digestible-starch diet (LDD) versus a highly digestible-starch diet (HDD) improves the capacity to oxidise starch, and that this is sex-dependent.					
31540385	1	28	theme	improved	171:178	arg1	metabolism					188:197	improved glucose metabolism	171:197	improved glucose metabolism	171:197	Starches of low digestibility are associated with improved glucose metabolism.					
31540385	0	29	theme	Digestible-Starch	8:24	arg1	Diet					26:29	A Lowly Digestible-Starch Diet	0:29	A Lowly Digestible-Starch Diet after Weaning	0:43	A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice.					
31540385	5	30	theme	whole-body	579:588	arg1	EE					610:611	EE	610:611	EE	610:611	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	30	theme	whole-body	579:588	arg1	expenditure					597:607	whole-body energy expenditure	579:607	whole-body energy expenditure (EE)	579:612	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	31	theme	indirect	751:758	arg1	calorimetry					760:770	extended indirect calorimetry	742:770	extended indirect calorimetry	742:770	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	1	32	theme	glucose	180:186	arg1	metabolism					188:197	improved glucose metabolism	171:197	improved glucose metabolism	171:197	Starches of low digestibility are associated with improved glucose metabolism.					
31540385	6	33	theme	hepatic	815:821	arg1	enrichment					823:832	total 13C hepatic enrichment	805:832	total 13C hepatic enrichment	805:832	Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus.					
31540385	5	34	theme	energy	590:595	arg1	EE					610:611	EE	610:611	EE	610:611	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	34	theme	energy	590:595	arg1	expenditure					597:607	whole-body energy expenditure	579:607	whole-body energy expenditure (EE)	579:612	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	0	35	from	Rate	82:85	arg1	Mice					115:118	Female, but Not in Male, Mice	90:118	Female, but Not in Male, Mice	90:118	A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice.					
31540385	9	36	theme	glucose	1381:1387	arg1	metabolism					1389:1398	glucose metabolism	1381:1398	glucose metabolism	1381:1398	These results show that starch digestibility impacts glucose metabolism differently in females versus males.					
31540385	5	37	theme	13C-labelled	699:710	arg1	bolus					719:723	an oral 13C-labelled starch bolus	691:723	an oral 13C-labelled starch bolus	691:723	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	6	38	theme	13C	811:813	arg1	enrichment					823:832	total 13C hepatic enrichment	805:832	total 13C hepatic enrichment	805:832	Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus.					
31540385	5	39	theme	oral	694:697	arg1	bolus					719:723	an oral 13C-labelled starch bolus	691:723	an oral 13C-labelled starch bolus	691:723	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	0	40	theme	Exogenous	54:62	arg1	Rate					82:85	Exogenous Glucose Oxidation Rate	54:85	Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice	54:118	A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice.					
31540385	6	41	theme	total	805:809	arg1	enrichment					823:832	total 13C hepatic enrichment	805:832	total 13C hepatic enrichment	805:832	Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus.					
31540385	5	42	theme	exchange	627:634	arg1	RER					643:645	RER	643:645	RER	643:645	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	42	theme	exchange	627:634	arg1	ratio					636:640	respiratory exchange ratio	615:640	respiratory exchange ratio (RER)	615:646	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	43	theme	hydrogen	649:656	arg1	production					658:667	hydrogen production	649:667	hydrogen production	649:667	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	5	44	theme	intervention	558:569	arg1	period					571:576	the intervention period	554:576	the intervention period	554:576	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	8	45	theme	total	1092:1096	arg1	oxidation					1111:1119	total carbohydrate oxidation	1092:1119	total carbohydrate oxidation	1092:1119	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	5	46	theme	period	571:576	arg1	end					547:549	the end	543:549	the end of the intervention period	543:576	At the end of the intervention period, whole-body energy expenditure (EE), respiratory exchange ratio (RER), hydrogen production, and the oxidation of an oral 13C-labelled starch bolus were measured by extended indirect calorimetry.					
31540385	8	47	theme	amylase	1269:1275	arg1	activity					1277:1284	amylase activity	1269:1284	amylase activity	1269:1284	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	8	48	theme	starch-derived	1125:1138	arg1	oxidation					1148:1156	starch-derived glucose oxidation	1125:1156	starch-derived glucose oxidation	1125:1156	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	4	49	theme	energy	496:501	arg1	intake					503:508	digestible energy intake	485:508	digestible energy intake (dEI)	485:514	Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly.					
31540385	4	49	theme	energy	496:501	arg1	dEI					511:513	dEI	511:513	dEI	511:513	Body weight (BW), body composition (BC), and digestible energy intake (dEI) were determined weekly.					
31540385	0	50	theme	Oxidation	72:80	arg1	Rate					82:85	Exogenous Glucose Oxidation Rate	54:85	Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice	54:118	A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice.					
31540385	2	51	theme	digestible-starch	228:244	arg1	LDD					252:254	LDD	252:254	LDD	252:254	We hypothesise that a lowly digestible-starch diet (LDD) versus a highly digestible-starch diet (HDD) improves the capacity to oxidise starch, and that this is sex-dependent.					
31540385	2	51	theme	digestible-starch	228:244	arg1	diet					246:249	a lowly digestible-starch diet	220:249	a lowly digestible-starch diet (LDD)	220:255	We hypothesise that a lowly digestible-starch diet (LDD) versus a highly digestible-starch diet (HDD) improves the capacity to oxidise starch, and that this is sex-dependent.					
31540385	6	52	theme	starch	916:921	arg1	bolus					923:927	the starch bolus	912:927	the starch bolus	912:927	Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus.					
31540385	8	53	dep	LDD	1205:1207	arg1	mice					1220:1223	mice	1220:1223	mice	1220:1223	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
31540385	7	54	theme	hydrogen	1028:1035	arg1	production					1037:1046	hydrogen production	1028:1046	hydrogen production	1028:1046	For both sexes, BW, BC, and basal EE and RER were not affected by the type of starch, but dEI and hydrogen production were increased by the LDD.					
31540385	0	55	theme	Glucose	64:70	arg1	Rate					82:85	Exogenous Glucose Oxidation Rate	54:85	Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice	54:118	A Lowly Digestible-Starch Diet after Weaning Enhances Exogenous Glucose Oxidation Rate in Female, but Not in Male, Mice.					
31540385	6	56	theme	amylase	784:790	arg1	activity					792:799	Pancreatic amylase activity	773:799	Pancreatic amylase activity	773:799	Pancreatic amylase activity and total 13C hepatic enrichment were determined in females immediately before and 4 h after administration of the starch bolus.					
31540385	8	57	theme	carbohydrate	1098:1109	arg1	oxidation					1111:1119	total carbohydrate oxidation	1092:1119	total carbohydrate oxidation	1092:1119	Only in females, total carbohydrate oxidation and starch-derived glucose oxidation in response to the starch bolus were higher in LDD versus HDD mice; this was not accompanied by differences in amylase activity or hepatic partitioning of the 13C label.					
30007674	8	0	theme	similar	1536:1542	arg1	appearances					1544:1554	similar appearances	1536:1554	similar appearances	1536:1554	Initially, both critical and excess emulsions were physically stable and had similar appearances.					
30007674	2	1	theme	supplement	267:276	arg1	products					298:305	food, supplement, and pharmaceutical products	261:305	food, supplement, and pharmaceutical products	261:305	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
30007674	9	2	theme	curcumin	1575:1582	arg1	degradation					1584:1594	curcumin degradation	1575:1594	curcumin degradation during storage	1575:1609	In all emulsions, curcumin degradation during storage occurred more rapidly at pH 7 than at pH 3, and was faster at 55 °C than at 37 °C.					
30007674	6	3	theme	other	1201:1205	arg1	emulsifiers					1207:1217	the other emulsifiers	1197:1217	the other emulsifiers	1197:1217	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	2	4	theme	food	261:264	arg1	products					298:305	food, supplement, and pharmaceutical products	261:305	food, supplement, and pharmaceutical products	261:305	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
30007674	1	5	theme	several	180:186	arg1	cancer					225:230	cancer	225:230	cancer	225:230	Oral ingestion of curcumin is claimed to be effective against several diseases, including inflammation and cancer.					
30007674	1	5	theme	several	180:186	arg1	diseases					188:195	several diseases	180:195	several diseases	180:195	Oral ingestion of curcumin is claimed to be effective against several diseases, including inflammation and cancer.					
30007674	1	5	theme	several	180:186	arg1	inflammation					208:219	inflammation	208:219	inflammation	208:219	Oral ingestion of curcumin is claimed to be effective against several diseases, including inflammation and cancer.					
30007674	4	6	theme	emulsifier	628:637	arg1	type					639:642	emulsifier type	628:642	emulsifier type	628:642	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	15	7	theme	physical	2308:2315	arg1	stability					2330:2338	the physical and chemical stability	2304:2338	the physical and chemical stability of curcumin-loaded emulsions	2304:2367	These results suggest that the physical and chemical stability of curcumin-loaded emulsions is influenced by emulsifier type and level.					
30007674	1	8	theme	Oral	118:121	arg1	ingestion					123:131	Oral ingestion	118:131	Oral ingestion of curcumin	118:143	Oral ingestion of curcumin is claimed to be effective against several diseases, including inflammation and cancer.					
30007674	12	9	theme	saponin	2018:2024	arg1	emulsions					2026:2034	saponin emulsions	2018:2034	saponin emulsions	2018:2034	Moreover, droplet creaming was observed in the critical Tween 80 and saponin emulsions, but not in the other emulsions.					
30007674	11	10	theme	curcumin	1847:1854	arg1	degradation					1856:1866	curcumin degradation	1847:1866	curcumin degradation	1847:1866	After storage at 55 °C for 15 days, the extent of curcumin degradation decreased in the following order: saponins > > gum arabic ≈ casinate ≈ Tween 80.					
30007674	4	11	theme	sodium	718:723	arg1	caseinate					725:733	sodium caseinate	718:733	the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic	647:773	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	13	12	theme	peroxidation	2177:2188	arg1	reactions					2190:2198	peroxidation reactions	2177:2198	peroxidation reactions	2177:2198	These results suggest that saponin accelerated curcumin degradation, possibly due to its ability to promote peroxidation reactions.					
30007674	14	13	theme	curcumin	2255:2262	arg1	degradation					2264:2274	curcumin degradation	2255:2274	curcumin degradation	2255:2274	Emulsifier concentration did not significantly affect curcumin degradation.					
30007674	6	14	theme	following	1005:1013	arg1	order					1015:1019	the following order	1001:1019	the following order	1001:1019	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	6	15	theme	gum	1150:1152	arg1	arabic					1154:1159	much more gum arabic	1140:1159	much more gum arabic	1140:1159	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	4	16	theme	curcumin-loaded	678:692	arg1	emulsions					707:715	curcumin-loaded oil-in-water emulsions	678:715	curcumin-loaded oil-in-water emulsions	678:715	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	5	17	from	effectiveness	780:792	arg1	emulsions					837:845	forming emulsions	829:845	forming emulsions	829:845	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	15	18	theme	emulsifier	2386:2395	arg1	type					2397:2400	emulsifier type	2386:2400	emulsifier type	2386:2400	These results suggest that the physical and chemical stability of curcumin-loaded emulsions is influenced by emulsifier type and level.					
30007674	16	19	theme	improved	2510:2517	arg1	properties					2550:2559	improved physicochemical and functional properties	2510:2559	improved physicochemical and functional properties	2510:2559	This information may be useful for formulating emulsion-based delivery systems for curcumin with improved physicochemical and functional properties.					
30007674	4	20	theme	emulsions	707:715	arg1	formation					651:659	formation	651:659	formation	651:659	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	4	20	theme	emulsions	707:715	arg1	stability					665:673	stability	665:673	stability	665:673	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	0	21	theme	type	70:73	arg1	Impact					49:54	Impact	49:54	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.	0:116	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.					
30007674	15	22	theme	emulsions	2359:2367	arg1	stability					2330:2338	the physical and chemical stability	2304:2338	the physical and chemical stability of curcumin-loaded emulsions	2304:2367	These results suggest that the physical and chemical stability of curcumin-loaded emulsions is influenced by emulsifier type and level.					
30007674	5	23	theme	load	912:915	arg1	terms					889:893	terms	889:893	terms of their surface load, i.e., the mass of emulsifier per unit surface area	889:967	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	0	24	from	Stability	0:8	arg1	emulsions					38:46	oil-in-water emulsions	25:46	oil-in-water emulsions	25:46	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.					
30007674	14	25	theme	Emulsifier	2201:2210	arg1	concentration					2212:2224	Emulsifier concentration	2201:2224	Emulsifier concentration	2201:2224	Emulsifier concentration did not significantly affect curcumin degradation.					
30007674	0	26	theme	concentration	79:91	arg1	Impact					49:54	Impact	49:54	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.	0:116	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.					
30007674	11	27	theme	saponins > > gum	1902:1917	arg1	arabic ≈ casinate ≈ Tween					1919:1943	saponins > > gum arabic ≈ casinate ≈ Tween 80	1902:1946	saponins > > gum arabic ≈ casinate ≈ Tween 80	1902:1946	After storage at 55 °C for 15 days, the extent of curcumin degradation decreased in the following order: saponins > > gum arabic ≈ casinate ≈ Tween 80.					
30007674	5	28	theme	surface	956:962	arg1	area					964:967	unit surface area	951:967	unit surface area	951:967	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	3	29	theme	Emulsion-based	423:436	arg1	systems					447:453	Emulsion-based delivery systems	423:453	Emulsion-based delivery systems	423:453	Emulsion-based delivery systems can be designed to overcome these challenges, but their composition and structure must be optimized to ensure they function appropriately.					
30007674	0	30	theme	chemical	96:103	arg1	degradation					105:115	chemical degradation	96:115	chemical degradation	96:115	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.					
30007674	15	31	theme	chemical	2321:2328	arg1	stability					2330:2338	the physical and chemical stability	2304:2338	the physical and chemical stability of curcumin-loaded emulsions	2304:2367	These results suggest that the physical and chemical stability of curcumin-loaded emulsions is influenced by emulsifier type and level.					
30007674	6	32	theme	 > > saponins	1045:1057	arg1	1.5 mg/m2					1107:1115	1.5 mg/m2	1107:1115	1.5 mg/m2	1107:1115	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	6	32	theme	 > > saponins	1045:1057	arg1	 > caseinate					1093:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate	1022:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2)	1022:1116	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	0	33	theme	emulsifier	59:68	arg1	type					70:73	emulsifier type	59:73	emulsifier type	59:73	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.					
30007674	2	34	theme	high	362:365	arg1	instability					376:386	high chemical instability	362:386	high chemical instability	362:386	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
30007674	5	35	theme	food-grade	803:812	arg1	emulsifiers					814:824	these food-grade emulsifiers	797:824	these food-grade emulsifiers at forming emulsions	797:845	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	6	36	theme	55.3 mg/m2	1034:1043	arg1	1.5 mg/m2					1107:1115	1.5 mg/m2	1107:1115	1.5 mg/m2	1107:1115	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	6	36	theme	55.3 mg/m2	1034:1043	arg1	 > caseinate					1093:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate	1022:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2)	1022:1116	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	10	37	theme	chemical	1711:1718	arg1	stability					1720:1728	The physical and chemical stability	1694:1728	The physical and chemical stability of the curcumin-loaded emulsions	1694:1761	The physical and chemical stability of the curcumin-loaded emulsions also depended on emulsifier type.					
30007674	4	38	theme	quillaja	746:753	arg1	saponin					755:761	quillaja saponin	746:761	the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic	647:773	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	2	39	theme	oral	401:404	arg1	bioavailability					406:420	limited oral bioavailability	393:420	limited oral bioavailability	393:420	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
30007674	0	40	theme	curcumin	13:20	arg1	Stability					0:8	Stability	0:8	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.	0:116	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.					
30007674	6	41	theme	arabic	1026:1031	arg1	1.5 mg/m2					1107:1115	1.5 mg/m2	1107:1115	1.5 mg/m2	1107:1115	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	6	41	theme	arabic	1026:1031	arg1	 > caseinate					1093:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate	1022:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2)	1022:1116	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	10	42	theme	emulsions	1753:1761	arg1	stability					1720:1728	The physical and chemical stability	1694:1728	The physical and chemical stability of the curcumin-loaded emulsions	1694:1761	The physical and chemical stability of the curcumin-loaded emulsions also depended on emulsifier type.					
30007674	0	43	theme	oil-in-water	25:36	arg1	emulsions					38:46	oil-in-water emulsions	25:46	oil-in-water emulsions	25:46	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.					
30007674	8	44	theme	critical	1475:1482	arg1	emulsions					1495:1503	both critical and excess emulsions	1470:1503	both critical and excess emulsions	1470:1503	Initially, both critical and excess emulsions were physically stable and had similar appearances.					
30007674	16	45	theme	delivery	2475:2482	arg1	systems					2484:2490	emulsion-based delivery systems	2460:2490	emulsion-based delivery systems for curcumin with improved physicochemical and functional properties	2460:2559	This information may be useful for formulating emulsion-based delivery systems for curcumin with improved physicochemical and functional properties.					
30007674	12	46	dep	Tween	2005:2009	arg1	the					1992:1994	the	1992:1994	the	1992:1994	Moreover, droplet creaming was observed in the critical Tween 80 and saponin emulsions, but not in the other emulsions.					
30007674	8	47	theme	excess	1488:1493	arg1	emulsions					1495:1503	both critical and excess emulsions	1470:1503	both critical and excess emulsions	1470:1503	Initially, both critical and excess emulsions were physically stable and had similar appearances.					
30007674	10	48	theme	emulsifier	1780:1789	arg1	type					1791:1794	emulsifier type	1780:1794	emulsifier type	1780:1794	The physical and chemical stability of the curcumin-loaded emulsions also depended on emulsifier type.					
30007674	16	49	with	curcumin	2496:2503	arg1	properties					2550:2559	improved physicochemical and functional properties	2510:2559	improved physicochemical and functional properties	2510:2559	This information may be useful for formulating emulsion-based delivery systems for curcumin with improved physicochemical and functional properties.					
30007674	7	50	theme	Curcumin-loaded	1220:1234	arg1	emulsions					1236:1244	Curcumin-loaded emulsions	1220:1244	Curcumin-loaded emulsions	1220:1244	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	7	51	theme	emulsifier	1415:1424	arg1	excess					1405:1410	an excess	1402:1410	an excess of emulsifier	1402:1424	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	0	52	dep	Stability	0:8	arg1	Impact					49:54	Impact	49:54	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.	0:116	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.					
30007674	6	53	theme	 > Tween	1070:1077	arg1	1.5 mg/m2					1107:1115	1.5 mg/m2	1107:1115	1.5 mg/m2	1107:1115	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	6	53	theme	 > Tween	1070:1077	arg1	 > caseinate					1093:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate	1022:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2)	1022:1116	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	2	54	theme	poor	339:342	arg1	solubility					350:359	its poor water solubility	335:359	its poor water solubility	335:359	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
30007674	6	55	theme	2.0 mg/m2	1060:1068	arg1	1.5 mg/m2					1107:1115	1.5 mg/m2	1107:1115	1.5 mg/m2	1107:1115	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	6	55	theme	2.0 mg/m2	1060:1068	arg1	 > caseinate					1093:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate	1022:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2)	1022:1116	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	5	56	dep	mass	928:931	arg1	i.e.					918:921	i.e.	918:921	i.e.	918:921	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	11	57	theme	following	1885:1893	arg1	order					1895:1899	the following order	1881:1899	the following order	1881:1899	After storage at 55 °C for 15 days, the extent of curcumin degradation decreased in the following order: saponins > > gum arabic ≈ casinate ≈ Tween 80.					
30007674	12	58	theme	droplet	1959:1965	arg1	creaming					1967:1974	droplet creaming	1959:1974	droplet creaming	1959:1974	Moreover, droplet creaming was observed in the critical Tween 80 and saponin emulsions, but not in the other emulsions.					
30007674	2	59	theme	pharmaceutical	283:296	arg1	products					298:305	food, supplement, and pharmaceutical products	261:305	food, supplement, and pharmaceutical products	261:305	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
30007674	5	60	theme	forming	829:835	arg1	emulsions					837:845	forming emulsions	829:845	forming emulsions	829:845	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	7	61	theme	critical	1353:1360	arg1	"					1361:1361	"critical"	1352:1361	"critical"	1352:1361	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	7	61	theme	critical	1353:1360	arg1	surfaces					1342:1349	the droplet surfaces	1330:1349	the droplet surfaces ("critical")	1330:1362	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	10	62	theme	physical	1698:1705	arg1	stability					1720:1728	The physical and chemical stability	1694:1728	The physical and chemical stability of the curcumin-loaded emulsions	1694:1761	The physical and chemical stability of the curcumin-loaded emulsions also depended on emulsifier type.					
30007674	11	63	from	55 °C	1814:1818	arg1	storage					1803:1809	storage	1803:1809	storage at 55 °C for 15 days	1803:1830	After storage at 55 °C for 15 days, the extent of curcumin degradation decreased in the following order: saponins > > gum arabic ≈ casinate ≈ Tween 80.					
30007674	4	64	theme	type	639:642	arg1	impact					618:623	the impact	614:623	the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic	614:773	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	7	65	theme	droplet	1334:1340	arg1	"					1361:1361	"critical"	1352:1361	"critical"	1352:1361	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	7	65	theme	droplet	1334:1340	arg1	surfaces					1342:1349	the droplet surfaces	1330:1349	the droplet surfaces ("critical")	1330:1362	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	5	66	from	emulsions	837:845	arg1	effectiveness					780:792	The effectiveness	776:792	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization	776:866	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	5	66	from	emulsions	837:845	arg1	emulsifiers					814:824	these food-grade emulsifiers	797:824	these food-grade emulsifiers at forming emulsions	797:845	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	4	67	from	impact	618:623	arg1	formation					651:659	formation	651:659	formation	651:659	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	4	67	from	impact	618:623	arg1	stability					665:673	stability	665:673	stability	665:673	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	11	68	theme	degradation	1856:1866	arg1	extent					1837:1842	the extent	1833:1842	the extent of curcumin degradation	1833:1866	After storage at 55 °C for 15 days, the extent of curcumin degradation decreased in the following order: saponins > > gum arabic ≈ casinate ≈ Tween 80.					
30007674	1	69	theme	curcumin	136:143	arg1	ingestion					123:131	Oral ingestion	118:131	Oral ingestion of curcumin	118:143	Oral ingestion of curcumin is claimed to be effective against several diseases, including inflammation and cancer.					
30007674	16	70	theme	physicochemical	2519:2533	arg1	properties					2550:2559	improved physicochemical and functional properties	2510:2559	improved physicochemical and functional properties	2510:2559	This information may be useful for formulating emulsion-based delivery systems for curcumin with improved physicochemical and functional properties.					
30007674	6	71	theme	more	1145:1148	arg1	arabic					1154:1159	much more gum arabic	1140:1159	much more gum arabic	1140:1159	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	5	72	theme	surface	904:910	arg1	load					912:915	their surface load	898:915	their surface load	898:915	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	16	73	theme	functional	2539:2548	arg1	properties					2550:2559	improved physicochemical and functional properties	2510:2559	improved physicochemical and functional properties	2510:2559	This information may be useful for formulating emulsion-based delivery systems for curcumin with improved physicochemical and functional properties.					
30007674	4	74	theme	oil-in-water	694:705	arg1	emulsions					707:715	curcumin-loaded oil-in-water emulsions	678:715	curcumin-loaded oil-in-water emulsions	678:715	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	5	75	theme	unit	951:954	arg1	area					964:967	unit surface area	951:967	unit surface area	951:967	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	6	76	theme	surface	974:980	arg1	loads					982:986	The surface loads	970:986	The surface loads	970:986	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	0	77	from	Impact	49:54	arg1	degradation					105:115	chemical degradation	96:115	chemical degradation	96:115	Stability of curcumin in oil-in-water emulsions: Impact of emulsifier type and concentration on chemical degradation.					
30007674	8	78	contain	had	1532:1534	arg2	appearances					1544:1554	similar appearances	1536:1554	similar appearances	1536:1554	Initially, both critical and excess emulsions were physically stable and had similar appearances.					
30007674	8	78	contain	had	1532:1534	arg1	emulsions					1495:1503	both critical and excess emulsions	1470:1503	both critical and excess emulsions	1470:1503	Initially, both critical and excess emulsions were physically stable and had similar appearances.					
30007674	15	79	theme	curcumin-loaded	2343:2357	arg1	emulsions					2359:2367	curcumin-loaded emulsions	2343:2367	curcumin-loaded emulsions	2343:2367	These results suggest that the physical and chemical stability of curcumin-loaded emulsions is influenced by emulsifier type and level.					
30007674	4	80	theme	gum	764:766	arg1	arabic					768:773	gum arabic	764:773	the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic	647:773	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	2	81	theme	chemical	367:374	arg1	instability					376:386	high chemical instability	362:386	high chemical instability	362:386	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
30007674	5	82	theme	emulsifiers	814:824	arg1	effectiveness					780:792	The effectiveness	776:792	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization	776:866	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	12	83	located	observed	1980:1987	arg1	Tween					2005:2009	Tween	2005:2009	Tween	2005:2009	Moreover, droplet creaming was observed in the critical Tween 80 and saponin emulsions, but not in the other emulsions.					
30007674	12	83	located	observed	1980:1987	arg2	creaming					1967:1974	droplet creaming	1959:1974	droplet creaming	1959:1974	Moreover, droplet creaming was observed in the critical Tween 80 and saponin emulsions, but not in the other emulsions.					
30007674	5	84	theme	emulsifier	936:945	arg1	mass					928:931	the mass	924:931	the mass of emulsifier per unit surface area	924:967	The effectiveness of these food-grade emulsifiers at forming emulsions by microfluidization was characterized in terms of their surface load, i.e., the mass of emulsifier per unit surface area.					
30007674	2	85	theme	limited	393:399	arg1	bioavailability					406:420	limited oral bioavailability	393:420	limited oral bioavailability	393:420	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
30007674	2	86	from	utilization	246:256	arg1	products					298:305	food, supplement, and pharmaceutical products	261:305	food, supplement, and pharmaceutical products	261:305	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
30007674	6	87	theme	gum	1022:1024	arg1	1.5 mg/m2					1107:1115	1.5 mg/m2	1107:1115	1.5 mg/m2	1107:1115	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	6	87	theme	gum	1022:1024	arg1	 > caseinate					1093:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate	1022:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2)	1022:1116	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	10	88	theme	curcumin-loaded	1737:1751	arg1	emulsions					1753:1761	the curcumin-loaded emulsions	1733:1761	the curcumin-loaded emulsions	1733:1761	The physical and chemical stability of the curcumin-loaded emulsions also depended on emulsifier type.					
30007674	7	89	theme	excess	1449:1454	arg1	phase					1441:1445	the aqueous phase	1429:1445	the aqueous phase ("excess")	1429:1456	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	7	89	theme	excess	1449:1454	arg1	"					1455:1455	"excess"	1448:1455	"excess"	1448:1455	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	13	90	theme	curcumin	2116:2123	arg1	degradation					2125:2135	curcumin degradation	2116:2135	curcumin degradation	2116:2135	These results suggest that saponin accelerated curcumin degradation, possibly due to its ability to promote peroxidation reactions.					
30007674	16	91	theme	emulsion-based	2460:2473	arg1	systems					2484:2490	emulsion-based delivery systems	2460:2490	emulsion-based delivery systems for curcumin with improved physicochemical and functional properties	2460:2559	This information may be useful for formulating emulsion-based delivery systems for curcumin with improved physicochemical and functional properties.					
30007674	6	92	theme	1.6 mg/m2	1083:1091	arg1	1.5 mg/m2					1107:1115	1.5 mg/m2	1107:1115	1.5 mg/m2	1107:1115	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	6	92	theme	1.6 mg/m2	1083:1091	arg1	 > caseinate					1093:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate	1022:1104	gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2)	1022:1116	The surface loads decreased in the following order: gum arabic (55.3 mg/m2) > > saponins (2.0 mg/m2) > Tween 80 (1.6 mg/m2) > caseinate (1.5 mg/m2), which indicated that much more gum arabic was required to form emulsions than the other emulsifiers.					
30007674	7	93	theme	aqueous	1433:1439	arg1	phase					1441:1445	the aqueous phase	1429:1445	the aqueous phase ("excess")	1429:1456	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	7	93	theme	aqueous	1433:1439	arg1	"					1455:1455	"excess"	1448:1455	"excess"	1448:1455	Curcumin-loaded emulsions were then prepared under conditions where there was just enough emulsifier to cover the droplet surfaces ("critical"), and under conditions where there was an excess of emulsifier in the aqueous phase ("excess").					
30007674	4	94	dep	formation	651:659	arg1	Tween					736:740	Tween	736:740	Tween	736:740	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	4	94	dep	formation	651:659	arg1	caseinate					725:733	sodium caseinate	718:733	the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic	647:773	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	4	94	dep	formation	651:659	arg1	saponin					755:761	quillaja saponin	746:761	the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic	647:773	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	4	94	dep	formation	651:659	arg1	the					647:649	the	647:649	the	647:649	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	4	94	dep	formation	651:659	arg1	arabic					768:773	gum arabic	764:773	the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic	647:773	This study examined the impact of emulsifier type on the formation and stability of curcumin-loaded oil-in-water emulsions: sodium caseinate; Tween 80; quillaja saponin; gum arabic.					
30007674	3	95	theme	delivery	438:445	arg1	systems					447:453	Emulsion-based delivery systems	423:453	Emulsion-based delivery systems	423:453	Emulsion-based delivery systems can be designed to overcome these challenges, but their composition and structure must be optimized to ensure they function appropriately.					
30007674	12	96	theme	other	2052:2056	arg1	emulsions					2058:2066	the other emulsions	2048:2066	the other emulsions	2048:2066	Moreover, droplet creaming was observed in the critical Tween 80 and saponin emulsions, but not in the other emulsions.					
30007674	2	97	theme	water	344:348	arg1	solubility					350:359	its poor water solubility	335:359	its poor water solubility	335:359	However, its utilization in food, supplement, and pharmaceutical products is often challenging due to its poor water solubility, high chemical instability, and limited oral bioavailability.					
31706777	6	0	dep	MCO	1227:1229	arg1	the					1223:1225	the	1223:1225	the	1223:1225	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	6	0	dep	MCO	1227:1229	arg1	diets					1239:1243	diets	1239:1243	diets	1239:1243	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	9	1	theme	genes	1575:1579	arg1	expression					1561:1570	lower mRNA expression	1550:1570	lower mRNA expression of genes related to protein, glycogen and lipid metabolism	1550:1629	The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet.					
31706777	6	2	theme	mixtures	1156:1163	arg1	efficiencies					1140:1151	efficiencies	1140:1151	efficiencies	1140:1151	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	6	2	theme	mixtures	1156:1163	arg1	effects					1128:1134	the binding effects	1116:1134	the binding effects	1116:1134	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	6	2	theme	mixtures	1156:1163	arg1	MCO					1227:1229	MCO	1227:1229	MCO	1227:1229	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	6	2	theme	mixtures	1156:1163	arg1	HCO					1235:1237	HCO	1235:1237	HCO	1235:1237	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	3	3	theme	side	542:545	arg1	effects					547:553	oxytetracycline-induced side effects	518:553	oxytetracycline-induced side effects	518:553	We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	11	4	theme	binding	1926:1932	arg1	effects					1934:1940	higher-oxytetracycline binding effects	1903:1940	higher-oxytetracycline binding effects	1903:1940	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	13	5	theme	omnivorous	2234:2243	arg1	species					2250:2256	omnivorous fish species	2234:2256	omnivorous fish species	2234:2256	Incorporating high carbohydrate in diet formulation for omnivorous fish species alleviates some of the side effects caused by antibiotics.					
31706777	11	6	theme	medium	1968:1973	arg1	starch					1975:1980	the medium starch	1964:1980	the medium starch with oxytetracyline and MCO diet	1964:2013	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	11	7	contain	had	1899:1901	arg2	efficiencies					1946:1957	efficiencies	1946:1957	efficiencies	1946:1957	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	11	7	contain	had	1899:1901	arg1	starch					1858:1863	The high starch	1849:1863	The high starch with oxytetracycline and HCO diet	1849:1897	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	11	7	contain	had	1899:1901	arg2	effects					1934:1940	higher-oxytetracycline binding effects	1903:1940	higher-oxytetracycline binding effects	1903:1940	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	4	8	theme	medium	625:630	arg1	carbohydrate					632:643	medium carbohydrate	625:643	medium carbohydrate (MC; 335 g/kg)	625:658	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	8	theme	medium	625:630	arg1	MC					646:647	MC	646:647	MC; 335 g/kg	646:657	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	10	9	theme	Feeding	1670:1676	arg1	O. niloticus					1678:1689	Feeding O. niloticus	1670:1689	Feeding O. niloticus on the HCO diet	1670:1705	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	1	10	theme	fish	213:216	arg1	health					218:223	fish health	213:223	fish health	213:223	Antibiotics used in global aquaculture production cause various side effects, which impair fish health.					
31706777	7	11	theme	nutrients	1315:1323	arg1	utilization					1325:1335	nutrients utilization	1315:1335	nutrients utilization	1315:1335	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	13	12	theme	high	2192:2195	arg1	carbohydrate					2197:2208	Incorporating high carbohydrate	2178:2208	Incorporating high carbohydrate in diet formulation for omnivorous fish species	2178:2256	Incorporating high carbohydrate in diet formulation for omnivorous fish species alleviates some of the side effects caused by antibiotics.					
31706777	10	13	theme	HCO	1698:1700	arg1	diet					1702:1705	the HCO diet	1694:1705	the HCO diet	1694:1705	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	5	14	theme	hepatic	861:867	arg1	nutrients					869:877	hepatic nutrients composition and metabolism	861:904	hepatic nutrients composition and metabolism	861:904	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	5	14	theme	hepatic	861:867	arg1	metabolism					895:904	metabolism	895:904	metabolism	895:904	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	5	14	theme	hepatic	861:867	arg1	composition					879:889	composition	879:889	composition	879:889	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	11	15	theme	MCO	2006:2008	arg1	diet					2010:2013	MCO diet	2006:2013	MCO diet	2006:2013	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	7	16	theme	lower	1296:1300	arg1	rate					1309:1312	lower growth rate	1296:1312	lower growth rate	1296:1312	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	6	17	theme	high	1179:1182	arg1	starch					1184:1189	high starch	1179:1189	high starch	1179:1189	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	2	18	theme	composition	254:264	arg1	use					239:241	the use	235:241	the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics,	235:394	However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear.					
31706777	1	19	theme	global	142:147	arg1	production					161:170	global aquaculture production	142:170	global aquaculture production	142:170	Antibiotics used in global aquaculture production cause various side effects, which impair fish health.					
31706777	6	20	with	starch	1184:1189	arg1	oxytetracycline					1196:1210	oxytetracycline	1196:1210	oxytetracycline	1196:1210	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	6	21	theme	medium	1168:1173	arg1	mixtures					1156:1163	mixtures	1156:1163	mixtures of medium and high starch with oxytetracycline	1156:1210	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	4	22	theme	high	664:667	arg1	carbohydrate					669:680	high carbohydrate	664:680	high carbohydrate (HC; 455 g/kg)	664:695	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	22	theme	high	664:667	arg1	HC					683:684	HC	683:684	HC; 455 g/kg	683:694	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	0	23	theme	Oreochromis	56:66	arg1	tilapia					47:53	Nile tilapia	42:53	Nile tilapia (Oreochromis niloticus)	42:77	High carbohydrate diet partially protects Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	0	23	theme	Oreochromis	56:66	arg1	niloticus					68:76	Oreochromis niloticus	56:76	Oreochromis niloticus	56:76	High carbohydrate diet partially protects Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	11	24	theme	HCO	1890:1892	arg1	diet					1894:1897	HCO diet	1890:1897	HCO diet	1890:1897	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	10	25	from	O. niloticus	1678:1689	arg1	diet					1702:1705	the HCO diet	1694:1705	the HCO diet	1694:1705	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	1	26	theme	various	178:184	arg1	effects					191:197	various side effects	178:197	various side effects	178:197	Antibiotics used in global aquaculture production cause various side effects, which impair fish health.					
31706777	8	27	theme	hepatosomatic	1430:1442	arg1	index					1444:1448	hepatosomatic index	1430:1448	hepatosomatic index	1430:1448	Dietary HCO increased hepatosomatic index and hepatic protein content of O. niloticus than MCO diet.					
31706777	5	28	dep	nutrients	869:877	arg1	nutrients					869:877	hepatic nutrients composition and metabolism	861:904	hepatic nutrients composition and metabolism	861:904	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	5	28	dep	nutrients	869:877	arg1	metabolism					895:904	metabolism	895:904	metabolism	895:904	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	5	28	dep	nutrients	869:877	arg1	composition					879:889	composition	879:889	composition	879:889	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	4	29	dep	HC	683:684	arg1	455 g/kg					687:694	455 g/kg	687:694	HC; 455 g/kg	683:694	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	0	30	theme	oxytetracycline-induced	84:106	arg1	effects					113:119	oxytetracycline-induced side effects	84:119	oxytetracycline-induced side effects	84:119	High carbohydrate diet partially protects Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	13	31	from	carbohydrate	2197:2208	arg1	formulation					2218:2228	diet formulation	2213:2228	diet formulation for omnivorous fish species	2213:2256	Incorporating high carbohydrate in diet formulation for omnivorous fish species alleviates some of the side effects caused by antibiotics.					
31706777	4	32	theme	oxytetracycline	738:752	arg1	diet					730:733	2.00 g/kg diet	720:733	2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter	720:789	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	32	theme	oxytetracycline	738:752	arg1	weight/day					769:778	80 mg/kg body weight/day	755:778	80 mg/kg body weight/day	755:778	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	32	theme	oxytetracycline	738:752	arg1	HCO					800:802	HCO	800:802	HCO	800:802	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	32	theme	oxytetracycline	738:752	arg1	MCO					792:794	MCO	792:794	MCO	792:794	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	7	33	theme	HCO	1282:1284	arg1	diets					1286:1290	the MCO and HCO diets	1270:1290	the MCO and HCO diets	1270:1290	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	8	34	theme	protein	1462:1468	arg1	content					1470:1476	hepatic protein content	1454:1476	hepatic protein content	1454:1476	Dietary HCO increased hepatosomatic index and hepatic protein content of O. niloticus than MCO diet.					
31706777	0	35	theme	High	0:3	arg1	diet					18:21	High carbohydrate diet	0:21	High carbohydrate diet	0:21	High carbohydrate diet partially protects Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	13	36	theme	Incorporating	2178:2190	arg1	carbohydrate					2197:2208	Incorporating high carbohydrate	2178:2208	Incorporating high carbohydrate in diet formulation for omnivorous fish species	2178:2256	Incorporating high carbohydrate in diet formulation for omnivorous fish species alleviates some of the side effects caused by antibiotics.					
31706777	7	37	theme	MCO	1274:1276	arg1	diets					1286:1290	the MCO and HCO diets	1270:1290	the MCO and HCO diets	1270:1290	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	3	38	theme	high	442:445	arg1	diet					460:463	high carbohydrate diet	442:463	high carbohydrate diet	442:463	We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	5	39	theme	microbiota	907:916	arg1	abundance					918:926	microbiota abundance	907:926	microbiota abundance	907:926	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	13	40	theme	diet	2213:2216	arg1	formulation					2218:2228	diet formulation	2213:2228	diet formulation for omnivorous fish species	2213:2256	Incorporating high carbohydrate in diet formulation for omnivorous fish species alleviates some of the side effects caused by antibiotics.					
31706777	4	41	theme	Triplicate	556:565	arg1	O. niloticus					584:595	thirty O. niloticus	577:595	thirty O. niloticus (9.50 ± 0.08 g)	577:611	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	41	theme	Triplicate	556:565	arg1	9.50 ± 0.08 g					598:610	9.50 ± 0.08 g	598:610	9.50 ± 0.08 g	598:610	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	41	theme	Triplicate	556:565	arg1	groups					567:572	Triplicate groups	556:572	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g)	556:611	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	12	42	theme	side	2138:2141	arg1	effects					2143:2149	oxytetracycline-induced side effects	2114:2149	oxytetracycline-induced side effects	2114:2149	Our study demonstrates that, high carbohydrate partially protects O. niloticus from oxytetracycline-induced side effects by binding the antibiotic.					
31706777	2	43	theme	fish	331:334	arg1	diets					336:340	fish diets	331:340	fish diets	331:340	However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear.					
31706777	9	44	theme	HCO	1537:1539	arg1	diet					1541:1544	the HCO diet	1533:1544	the HCO diet	1533:1544	The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet.					
31706777	10	45	theme	cellular	1800:1807	arg1	bacteria					1756:1763	pathogenic bacteria	1745:1763	pathogenic bacteria	1745:1763	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	10	45	theme	cellular	1800:1807	arg1	stress					1809:1814	cellular stress	1800:1814	cellular stress	1800:1814	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	4	46	theme	80 mg/kg	755:762	arg1	diet					730:733	2.00 g/kg diet	720:733	2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter	720:789	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	46	theme	80 mg/kg	755:762	arg1	weight/day					769:778	80 mg/kg body weight/day	755:778	80 mg/kg body weight/day	755:778	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	3	47	theme	diet	460:463	arg1	ability					431:437	the ability	427:437	the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects	427:553	We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	7	48	contain	had	1292:1294	arg2	utilization					1325:1335	nutrients utilization	1315:1335	nutrients utilization	1315:1335	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	7	48	contain	had	1292:1294	arg2	rate					1309:1312	lower growth rate	1296:1312	lower growth rate	1296:1312	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	7	48	contain	had	1292:1294	arg1	O. niloticus					1250:1261	The O. niloticus	1246:1261	The O. niloticus fed on the MCO and HCO diets	1246:1290	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	7	48	contain	had	1292:1294	arg2	rate					1350:1353	survival rate	1341:1353	survival rate	1341:1353	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	6	49	from	mechanism	1047:1055	arg1	oxytetracycline					1087:1101	oxytetracycline	1087:1101	oxytetracycline	1087:1101	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	3	50	theme	Oreochromis	490:500	arg1	tilapia					481:487	Nile tilapia	476:487	Nile tilapia (Oreochromis niloticus)	476:511	We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	3	50	theme	Oreochromis	490:500	arg1	niloticus					502:510	Oreochromis niloticus	490:510	Oreochromis niloticus	490:510	We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	4	51	dep	MC	646:647	arg1	335 g/kg					650:657	335 g/kg	650:657	MC; 335 g/kg	646:657	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	6	52	theme	carbohydrate	1060:1071	arg1	protection					1073:1082	carbohydrate protection	1060:1082	carbohydrate protection	1060:1082	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	2	53	from	components	317:326	arg1	diets					336:340	fish diets	331:340	fish diets	331:340	However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear.					
31706777	3	54	theme	oxytetracycline-induced	518:540	arg1	effects					547:553	oxytetracycline-induced side effects	518:553	oxytetracycline-induced side effects	518:553	We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	7	55	theme	HC	1384:1385	arg1	diets					1387:1391	the MC and HC diets	1373:1391	the MC and HC diets	1373:1391	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	9	56	theme	related	1581:1587	arg1	genes					1575:1579	genes	1575:1579	genes related to protein, glycogen and lipid metabolism	1575:1629	The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet.					
31706777	4	57	theme	O. niloticus	584:595	arg1	O. niloticus					584:595	thirty O. niloticus	577:595	thirty O. niloticus (9.50 ± 0.08 g)	577:611	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	57	theme	O. niloticus	584:595	arg1	9.50 ± 0.08 g					598:610	9.50 ± 0.08 g	598:610	9.50 ± 0.08 g	598:610	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	57	theme	O. niloticus	584:595	arg1	groups					567:572	Triplicate groups	556:572	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g)	556:611	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	11	58	theme	higher-oxytetracycline	1903:1924	arg1	effects					1934:1940	higher-oxytetracycline binding effects	1903:1940	higher-oxytetracycline binding effects	1903:1940	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	1	59	theme	side	186:189	arg1	effects					191:197	various side effects	178:197	various side effects	178:197	Antibiotics used in global aquaculture production cause various side effects, which impair fish health.					
31706777	7	60	theme	MC	1377:1378	arg1	diets					1387:1391	the MC and HC diets	1373:1391	the MC and HC diets	1373:1391	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	10	61	theme	MCO	1839:1841	arg1	diet					1843:1846	the MCO diet	1835:1846	the MCO diet	1835:1846	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	6	62	theme	binding	1120:1126	arg1	effects					1128:1134	the binding effects	1116:1134	the binding effects	1116:1134	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	12	63	theme	high	2059:2062	arg1	carbohydrate					2064:2075	high carbohydrate	2059:2075	high carbohydrate	2059:2075	Our study demonstrates that, high carbohydrate partially protects O. niloticus from oxytetracycline-induced side effects by binding the antibiotic.					
31706777	5	64	theme	lipid	986:990	arg1	peroxidation					992:1003	lipid peroxidation	986:1003	lipid peroxidation	986:1003	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	9	65	theme	lipid	1614:1618	arg1	metabolism					1620:1629	lipid metabolism	1614:1629	lipid metabolism	1614:1629	The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet.					
31706777	7	66	theme	survival	1341:1348	arg1	rate					1350:1353	survival rate	1341:1353	survival rate	1341:1353	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	10	67	theme	innate	1717:1722	arg1	immunity					1724:1731	innate immunity	1717:1731	innate immunity	1717:1731	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	13	68	theme	side	2281:2284	arg1	effects					2286:2292	the side effects	2277:2292	the side effects caused by antibiotics	2277:2314	Incorporating high carbohydrate in diet formulation for omnivorous fish species alleviates some of the side effects caused by antibiotics.					
31706777	4	69	theme	carbohydrate	632:643	arg1	diets					697:701	medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets	625:701	medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO	625:802	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	9	70	contain	had	1546:1548	arg2	expression					1561:1570	lower mRNA expression	1550:1570	lower mRNA expression of genes related to protein, glycogen and lipid metabolism	1550:1629	The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet.					
31706777	9	70	contain	had	1546:1548	arg1	O. niloticus					1513:1524	The O. niloticus	1509:1524	The O. niloticus fed on the HCO diet	1509:1544	The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet.					
31706777	11	71	theme	high	1853:1856	arg1	starch					1858:1863	The high starch	1849:1863	The high starch with oxytetracycline and HCO diet	1849:1897	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	13	72	theme	fish	2245:2248	arg1	species					2250:2256	omnivorous fish species	2234:2256	omnivorous fish species	2234:2256	Incorporating high carbohydrate in diet formulation for omnivorous fish species alleviates some of the side effects caused by antibiotics.					
31706777	6	73	theme	starch	1184:1189	arg1	mixtures					1156:1163	mixtures	1156:1163	mixtures of medium and high starch with oxytetracycline	1156:1210	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	10	74	theme	pathogenic	1745:1754	arg1	pro-inflammation					1766:1781	pro-inflammation	1766:1781	pro-inflammation	1766:1781	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	10	74	theme	pathogenic	1745:1754	arg1	bacteria					1756:1763	pathogenic bacteria	1745:1763	pathogenic bacteria	1745:1763	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	10	74	theme	pathogenic	1745:1754	arg1	apoptosis					1820:1828	apoptosis	1820:1828	apoptosis	1820:1828	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	10	74	theme	pathogenic	1745:1754	arg1	hepatotoxicity					1784:1797	hepatotoxicity	1784:1797	hepatotoxicity	1784:1797	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	10	74	theme	pathogenic	1745:1754	arg1	stress					1809:1814	cellular stress	1800:1814	cellular stress	1800:1814	Feeding O. niloticus on the HCO diet increased innate immunity and reduced pathogenic bacteria, pro-inflammation, hepatotoxicity, cellular stress and apoptosis than the MCO diet.					
31706777	8	75	theme	Dietary	1408:1414	arg1	HCO					1416:1418	Dietary HCO	1408:1418	Dietary HCO	1408:1418	Dietary HCO increased hepatosomatic index and hepatic protein content of O. niloticus than MCO diet.					
31706777	7	76	theme	growth	1302:1307	arg1	rate					1309:1312	lower growth rate	1296:1312	lower growth rate	1296:1312	The O. niloticus fed on the MCO and HCO diets had lower growth rate, nutrients utilization and survival rate than those fed on the MC and HC diets, respectively.					
31706777	2	77	theme	components	317:326	arg1	one					297:299	one	297:299	one	297:299	However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear.					
31706777	2	77	theme	components	317:326	arg1	components					317:326	the dominant components	304:326	the dominant components in fish diets	304:340	However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear.					
31706777	2	78	theme	dietary	246:252	arg1	composition					254:264	dietary composition	246:264	dietary composition	246:264	However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear.					
31706777	1	79	theme	aquaculture	149:159	arg1	production					161:170	global aquaculture production	142:170	global aquaculture production	142:170	Antibiotics used in global aquaculture production cause various side effects, which impair fish health.					
31706777	9	80	theme	MCO	1660:1662	arg1	diet					1664:1667	the MCO diet	1656:1667	the MCO diet	1656:1667	The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet.					
31706777	11	81	with	starch	1858:1863	arg1	oxytetracycline					1870:1884	oxytetracycline	1870:1884	oxytetracycline	1870:1884	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	11	81	with	starch	1858:1863	arg1	diet					1894:1897	HCO diet	1890:1897	HCO diet	1890:1897	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	5	82	theme	growth	841:846	arg1	performance					848:858	growth performance	841:858	growth performance	841:858	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	6	83	with	medium	1168:1173	arg1	oxytetracycline					1196:1210	oxytetracycline	1196:1210	oxytetracycline	1196:1210	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	4	84	theme	carbohydrate	669:680	arg1	diets					697:701	medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets	625:701	medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO	625:802	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	0	85	theme	side	108:111	arg1	effects					113:119	oxytetracycline-induced side effects	84:119	oxytetracycline-induced side effects	84:119	High carbohydrate diet partially protects Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	8	86	theme	hepatic	1454:1460	arg1	content					1470:1476	hepatic protein content	1454:1476	hepatic protein content	1454:1476	Dietary HCO increased hepatosomatic index and hepatic protein content of O. niloticus than MCO diet.					
31706777	4	87	theme	2.00 g/kg	720:728	arg1	diet					730:733	2.00 g/kg diet	720:733	2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter	720:789	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	87	theme	2.00 g/kg	720:728	arg1	weight/day					769:778	80 mg/kg body weight/day	755:778	80 mg/kg body weight/day	755:778	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	6	88	theme	possible	1038:1045	arg1	mechanism					1047:1055	the possible mechanism	1034:1055	the possible mechanism of carbohydrate protection on oxytetracycline	1034:1101	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	0	89	theme	carbohydrate	5:16	arg1	diet					18:21	High carbohydrate diet	0:21	High carbohydrate diet	0:21	High carbohydrate diet partially protects Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	8	90	theme	O. niloticus	1481:1492	arg1	index					1444:1448	hepatosomatic index	1430:1448	hepatosomatic index	1430:1448	Dietary HCO increased hepatosomatic index and hepatic protein content of O. niloticus than MCO diet.					
31706777	8	90	theme	O. niloticus	1481:1492	arg1	content					1470:1476	hepatic protein content	1454:1476	hepatic protein content	1454:1476	Dietary HCO increased hepatosomatic index and hepatic protein content of O. niloticus than MCO diet.					
31706777	2	91	theme	side	359:362	arg1	effects					364:370	the side effects	355:370	the side effects induced by antibiotics	355:393	However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear.					
31706777	9	92	theme	lower	1550:1554	arg1	expression					1561:1570	lower mRNA expression	1550:1570	lower mRNA expression of genes related to protein, glycogen and lipid metabolism	1550:1629	The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet.					
31706777	11	93	with	starch	1975:1980	arg1	oxytetracyline					1987:2000	oxytetracyline	1987:2000	oxytetracyline	1987:2000	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	11	93	with	starch	1975:1980	arg1	diet					2010:2013	MCO diet	2006:2013	MCO diet	2006:2013	The high starch with oxytetracycline and HCO diet had higher-oxytetracycline binding effects and efficiencies than the medium starch with oxytetracyline and MCO diet, respectively.					
31706777	8	94	theme	MCO	1499:1501	arg1	diet					1503:1506	MCO diet	1499:1506	MCO diet	1499:1506	Dietary HCO increased hepatosomatic index and hepatic protein content of O. niloticus than MCO diet.					
31706777	3	95	theme	carbohydrate	447:458	arg1	diet					460:463	high carbohydrate diet	442:463	high carbohydrate diet	442:463	We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	0	96	theme	Nile	42:45	arg1	tilapia					47:53	Nile tilapia	42:53	Nile tilapia (Oreochromis niloticus)	42:77	High carbohydrate diet partially protects Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	0	96	theme	Nile	42:45	arg1	niloticus					68:76	Oreochromis niloticus	56:76	Oreochromis niloticus	56:76	High carbohydrate diet partially protects Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	12	97	theme	oxytetracycline-induced	2114:2136	arg1	effects					2143:2149	oxytetracycline-induced side effects	2114:2149	oxytetracycline-induced side effects	2114:2149	Our study demonstrates that, high carbohydrate partially protects O. niloticus from oxytetracycline-induced side effects by binding the antibiotic.					
31706777	3	98	theme	Nile	476:479	arg1	tilapia					481:487	Nile tilapia	476:487	Nile tilapia (Oreochromis niloticus)	476:511	We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	3	98	theme	Nile	476:479	arg1	niloticus					502:510	Oreochromis niloticus	490:510	Oreochromis niloticus	490:510	We determined the ability of high carbohydrate diet to protect Nile tilapia (Oreochromis niloticus) from oxytetracycline-induced side effects.					
31706777	9	99	theme	mRNA	1556:1559	arg1	expression					1561:1570	lower mRNA expression	1550:1570	lower mRNA expression of genes related to protein, glycogen and lipid metabolism	1550:1629	The O. niloticus fed on the HCO diet had lower mRNA expression of genes related to protein, glycogen and lipid metabolism compared to those fed on the MCO diet.					
31706777	5	100	theme	oxidative	939:947	arg1	hepatotoxicity					970:983	hepatotoxicity	970:983	hepatotoxicity	970:983	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	5	100	theme	oxidative	939:947	arg1	stress					962:967	oxidative and cellular stress	939:967	oxidative and cellular stress	939:967	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	2	101	theme	dominant	308:315	arg1	components					317:326	the dominant components	304:326	the dominant components in fish diets	304:340	However, the use of dietary composition such as carbohydrate, which is one of the dominant components in fish diets to attenuate the side effects induced by antibiotics, remains unclear.					
31706777	5	102	theme	cellular	953:960	arg1	hepatotoxicity					970:983	hepatotoxicity	970:983	hepatotoxicity	970:983	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	5	102	theme	cellular	953:960	arg1	stress					962:967	oxidative and cellular stress	939:967	oxidative and cellular stress	939:967	Thereafter, we assessed growth performance, hepatic nutrients composition and metabolism, microbiota abundance, immunity, oxidative and cellular stress, hepatotoxicity, lipid peroxidation and apoptosis.					
31706777	6	103	theme	protection	1073:1082	arg1	mechanism					1047:1055	the possible mechanism	1034:1055	the possible mechanism of carbohydrate protection on oxytetracycline	1034:1101	To understand the possible mechanism of carbohydrate protection on oxytetracycline, we assessed the binding effects and efficiencies of mixtures of medium and high starch with oxytetracycline as well as the MCO and HCO diets.					
31706777	4	104	theme	body	764:767	arg1	diet					730:733	2.00 g/kg diet	720:733	2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter	720:789	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
31706777	4	104	theme	body	764:767	arg1	weight/day					769:778	80 mg/kg body weight/day	755:778	80 mg/kg body weight/day	755:778	Triplicate groups of thirty O. niloticus (9.50 ± 0.08 g) were fed on medium carbohydrate (MC; 335 g/kg) and high carbohydrate (HC; 455 g/kg) diets without and with 2.00 g/kg diet of oxytetracycline (80 mg/kg body weight/day) hereafter, MCO and HCO for 35 days.					
30508660	4	0	theme	reactivity	565:574	arg1	order					556:560	an order	553:560	an order of reactivity	553:574	Kinetics study using 1H NMR spectroscopy showed an order of reactivity: O-xylopyranoside > O-arabinopyranoside > O-fucopyranoside.					
30508660	4	1	theme	Kinetics	505:512	arg1	study					514:518	Kinetics study	505:518	Kinetics study using 1H NMR spectroscopy	505:544	Kinetics study using 1H NMR spectroscopy showed an order of reactivity: O-xylopyranoside > O-arabinopyranoside > O-fucopyranoside.					
30508660	5	2	theme	Benzoylated	636:646	arg1	glycosides					648:657	Benzoylated glycosides	636:657	Benzoylated glycosides	636:657	Benzoylated glycosides were more reactive than acetylated glycosides.					
30508660	2	3	theme	some	297:300	arg1	anomerisation					280:292	Lewis acid promoted anomerisation	260:292	Lewis acid promoted anomerisation of some of their alkyl O- and S-glycopyranosides	260:341	Lewis acid promoted anomerisation of some of their alkyl O- and S-glycopyranosides is reported here.					
30508660	8	4	theme	β-d-xylopyranoside	945:962	arg1	flexible					1026:1033	flexible	1026:1033	flexible	1026:1033	The β-d-xylopyranoside and α-l-arabinopyranoside reactants are conformationally more flexible than β-l-fucopyranosides.					
30508660	8	4	theme	β-d-xylopyranoside	945:962	arg1	reactants					990:998	The β-d-xylopyranoside and α-l-arabinopyranoside reactants	941:998	The β-d-xylopyranoside and α-l-arabinopyranoside reactants	941:998	The β-d-xylopyranoside and α-l-arabinopyranoside reactants are conformationally more flexible than β-l-fucopyranosides.					
30508660	6	5	theme	S-xylopyranosides	843:859	arg1	reactivity					822:831	the reactivity	818:831	the reactivity of O- and S-xylopyranosides	818:859	The reactivity of S-glycosides was greater than that of O-glycosides for both arabinose and fucose derivatives; the reactivity of O- and S-xylopyranosides was similar.					
30508660	6	5	theme	S-xylopyranosides	843:859	arg1	similar					865:871	similar	865:871	similar	865:871	The reactivity of S-glycosides was greater than that of O-glycosides for both arabinose and fucose derivatives; the reactivity of O- and S-xylopyranosides was similar.					
30508660	3	6	theme	chloride	435:442	arg1	by-product					444:453	the glycosyl chloride by-product	422:453	the glycosyl chloride by-product	422:453	SnCl4 was more successful than TiCl4, with the latter giving the glycosyl chloride by-product in some cases, and both were superior to BF3OEt2.					
30508660	8	7	theme	α-l-arabinopyranoside	968:988	arg1	flexible					1026:1033	flexible	1026:1033	flexible	1026:1033	The β-d-xylopyranoside and α-l-arabinopyranoside reactants are conformationally more flexible than β-l-fucopyranosides.					
30508660	8	7	theme	α-l-arabinopyranoside	968:988	arg1	reactants					990:998	The β-d-xylopyranoside and α-l-arabinopyranoside reactants	941:998	The β-d-xylopyranoside and α-l-arabinopyranoside reactants	941:998	The β-d-xylopyranoside and α-l-arabinopyranoside reactants are conformationally more flexible than β-l-fucopyranosides.					
30508660	6	8	theme	O-	836:837	arg1	reactivity					822:831	the reactivity	818:831	the reactivity of O- and S-xylopyranosides	818:859	The reactivity of S-glycosides was greater than that of O-glycosides for both arabinose and fucose derivatives; the reactivity of O- and S-xylopyranosides was similar.					
30508660	6	8	theme	O-	836:837	arg1	similar					865:871	similar	865:871	similar	865:871	The reactivity of S-glycosides was greater than that of O-glycosides for both arabinose and fucose derivatives; the reactivity of O- and S-xylopyranosides was similar.					
30508660	3	9	with	successful	376:385	arg1	latter					408:413	latter	408:413	latter	408:413	SnCl4 was more successful than TiCl4, with the latter giving the glycosyl chloride by-product in some cases, and both were superior to BF3OEt2.					
30508660	0	10	theme	Lewis	0:4	arg1	acid					6:9	Lewis acid	0:9	Lewis acid	0:9	Lewis acid promoted anomerisation of alkyl O- and S-xylo-, arabino- and fucopyranosides.					
30508660	5	11	theme	acetylated	683:692	arg1	glycosides					694:703	acetylated glycosides	683:703	acetylated glycosides	683:703	Benzoylated glycosides were more reactive than acetylated glycosides.					
30508660	3	12	theme	glycosyl	426:433	arg1	by-product					444:453	the glycosyl chloride by-product	422:453	the glycosyl chloride by-product	422:453	SnCl4 was more successful than TiCl4, with the latter giving the glycosyl chloride by-product in some cases, and both were superior to BF3OEt2.					
30508660	2	13	theme	alkyl	311:315	arg1	O-					317:318	their alkyl O-	305:318	their alkyl O-	305:318	Lewis acid promoted anomerisation of some of their alkyl O- and S-glycopyranosides is reported here.					
30508660	6	14	theme	S-glycosides	724:735	arg1	greater					741:747	greater	741:747	greater	741:747	The reactivity of S-glycosides was greater than that of O-glycosides for both arabinose and fucose derivatives; the reactivity of O- and S-xylopyranosides was similar.					
30508660	6	14	theme	S-glycosides	724:735	arg1	reactivity					710:719	The reactivity	706:719	The reactivity of S-glycosides	706:735	The reactivity of S-glycosides was greater than that of O-glycosides for both arabinose and fucose derivatives; the reactivity of O- and S-xylopyranosides was similar.					
30508660	0	15	theme	alkyl	37:41	arg1	arabino-					59:66	arabino-	59:66	arabino-	59:66	Lewis acid promoted anomerisation of alkyl O- and S-xylo-, arabino- and fucopyranosides.					
30508660	0	15	theme	alkyl	37:41	arg1	O-					43:44	alkyl O-	37:44	alkyl O-	37:44	Lewis acid promoted anomerisation of alkyl O- and S-xylo-, arabino- and fucopyranosides.					
30508660	0	15	theme	alkyl	37:41	arg1	fucopyranosides					72:86	fucopyranosides	72:86	fucopyranosides	72:86	Lewis acid promoted anomerisation of alkyl O- and S-xylo-, arabino- and fucopyranosides.					
30508660	7	16	theme	highest	878:884	arg1	stereoselectivities					886:904	The highest stereoselectivities	874:904	The highest stereoselectivities	874:904	The highest stereoselectivities were observed for fucopyranosides.					
30508660	1	17	theme	natural	205:211	arg1	products					213:220	natural products	205:220	natural products	205:220	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	18	theme	products	213:220	arg1	6-deoxyhexopyranosides					109:130	6-deoxyhexopyranosides	109:130	6-deoxyhexopyranosides	109:130	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	18	theme	products	213:220	arg1	Pentopyranoside					89:103	Pentopyranoside	89:103	Pentopyranoside	89:103	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	18	theme	products	213:220	arg1	those					141:145	those	141:145	those	141:145	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	18	theme	products	213:220	arg1	components					191:200	components	191:200	components of natural products, oligosaccharides or polysaccharides	191:257	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	0	19	theme	O-	43:44	arg1	anomerisation					20:32	anomerisation	20:32	anomerisation of alkyl O- and S-xylo-, arabino- and fucopyranosides	20:86	Lewis acid promoted anomerisation of alkyl O- and S-xylo-, arabino- and fucopyranosides.					
30508660	4	20	theme	NMR	529:531	arg1	spectroscopy					533:544	1H NMR spectroscopy	526:544	1H NMR spectroscopy	526:544	Kinetics study using 1H NMR spectroscopy showed an order of reactivity: O-xylopyranoside > O-arabinopyranoside > O-fucopyranoside.					
30508660	4	21	theme	1H	526:527	arg1	spectroscopy					533:544	1H NMR spectroscopy	526:544	1H NMR spectroscopy	526:544	Kinetics study using 1H NMR spectroscopy showed an order of reactivity: O-xylopyranoside > O-arabinopyranoside > O-fucopyranoside.					
30508660	2	22	theme	promoted	271:278	arg1	anomerisation					280:292	Lewis acid promoted anomerisation	260:292	Lewis acid promoted anomerisation of some of their alkyl O- and S-glycopyranosides	260:341	Lewis acid promoted anomerisation of some of their alkyl O- and S-glycopyranosides is reported here.					
30508660	2	23	theme	acid	266:269	arg1	anomerisation					280:292	Lewis acid promoted anomerisation	260:292	Lewis acid promoted anomerisation of some of their alkyl O- and S-glycopyranosides	260:341	Lewis acid promoted anomerisation of some of their alkyl O- and S-glycopyranosides is reported here.					
30508660	1	24	theme	oligosaccharides	223:238	arg1	6-deoxyhexopyranosides					109:130	6-deoxyhexopyranosides	109:130	6-deoxyhexopyranosides	109:130	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	24	theme	oligosaccharides	223:238	arg1	Pentopyranoside					89:103	Pentopyranoside	89:103	Pentopyranoside	89:103	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	24	theme	oligosaccharides	223:238	arg1	those					141:145	those	141:145	those	141:145	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	24	theme	oligosaccharides	223:238	arg1	components					191:200	components	191:200	components of natural products, oligosaccharides or polysaccharides	191:257	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	2	25	theme	Lewis	260:264	arg1	anomerisation					280:292	Lewis acid promoted anomerisation	260:292	Lewis acid promoted anomerisation of some of their alkyl O- and S-glycopyranosides	260:341	Lewis acid promoted anomerisation of some of their alkyl O- and S-glycopyranosides is reported here.					
30508660	6	26	dep	arabinose	784:792	arg1	derivatives					805:815	derivatives	805:815	derivatives	805:815	The reactivity of S-glycosides was greater than that of O-glycosides for both arabinose and fucose derivatives; the reactivity of O- and S-xylopyranosides was similar.					
30508660	0	27	theme	S-xylo-	50:56	arg1	anomerisation					20:32	anomerisation	20:32	anomerisation of alkyl O- and S-xylo-, arabino- and fucopyranosides	20:86	Lewis acid promoted anomerisation of alkyl O- and S-xylo-, arabino- and fucopyranosides.					
30508660	1	28	theme	polysaccharides	243:257	arg1	6-deoxyhexopyranosides					109:130	6-deoxyhexopyranosides	109:130	6-deoxyhexopyranosides	109:130	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	28	theme	polysaccharides	243:257	arg1	Pentopyranoside					89:103	Pentopyranoside	89:103	Pentopyranoside	89:103	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	28	theme	polysaccharides	243:257	arg1	those					141:145	those	141:145	those	141:145	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
30508660	1	28	theme	polysaccharides	243:257	arg1	components					191:200	components	191:200	components of natural products, oligosaccharides or polysaccharides	191:257	Pentopyranoside and 6-deoxyhexopyranosides, such as those from d-xylose, l-arabinose and l-fucose are components of natural products, oligosaccharides or polysaccharides.					
31232851	6	0	dep	MEASUREMENTS	907:918	arg1	underwent					1048:1056	underwent	1048:1056	underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular	1048:1118	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	13	1	theme	pediatric	1916:1924	arg1	patients					1952:1959	pediatric ventricular assist device patients	1916:1959	pediatric ventricular assist device patients	1916:1959	CONCLUSIONS Antithrombin should be dosed on fat-free mass in pediatric ventricular assist device patients.					
31232851	10	2	theme	model	1653:1657	arg1	fit					1659:1661	a better model fit	1644:1661	a better model fit than actual body weight	1644:1685	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	9	3	dep	hours	1465:1469	arg1	8.8-41.6 hr					1493:1503	8.8-41.6 hr	1493:1503	8.8-41.6 hr	1493:1503	Antithrombin activity levels were drawn a median 19.9 hours (interquartile range, 8.8-41.6 hr) after antithrombin dose.					
31232851	9	3	dep	hours	1465:1469	arg1	range					1486:1490	interquartile range	1472:1490	interquartile range	1472:1490	Antithrombin activity levels were drawn a median 19.9 hours (interquartile range, 8.8-41.6 hr) after antithrombin dose.					
31232851	14	4	dep	patients	2116:2123	arg1	assist					2102:2107	assist	2102:2107	assist	2102:2107	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	10	5	theme	body	1675:1678	arg1	weight					1680:1685	actual body weight	1668:1685	actual body weight	1668:1685	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	13	6	dep	CONCLUSIONS	1855:1865	arg1	Antithrombin					1867:1878	Antithrombin	1867:1878	CONCLUSIONS Antithrombin	1855:1878	CONCLUSIONS Antithrombin should be dosed on fat-free mass in pediatric ventricular assist device patients.					
31232851	4	7	theme	antithrombin	696:707	arg1	dose					688:691	a dose	686:691	a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017	686:790	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	10	8	theme	one-compartment	1533:1547	arg1	model					1568:1572	A one-compartment proportional error model	1531:1572	A one-compartment proportional error model	1531:1572	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	6	9	theme	met	960:962	arg1	criteria					970:977	met study criteria	960:977	41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr])	948:1035	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	9	theme	met	960:962	arg1	age					987:989	median age	980:989	median age	980:989	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	10	theme	pediatric	1098:1106	arg1	ventricular					1108:1118	pediatric ventricular	1098:1118	the pulsatile Berlin EXCOR pediatric ventricular	1071:1118	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	5	11	theme	NONMEM	864:869	arg1	v.7.4					871:875	NONMEM v.7.4	864:875	NONMEM v.7.4 (Icon, PLC, Dublin, Ireland)	864:904	INTERVENTIONS Population pharmacokinetic analysis and simulation using NONMEM v.7.4 (Icon, PLC, Dublin, Ireland).					
31232851	8	12	theme	activity	1376:1383	arg1	levels					1385:1390	baseline antithrombin activity levels	1354:1390	baseline antithrombin activity levels	1354:1390	A total of 181 antithrombin doses (44.1 ± 24.6 U/kg/dose) were included, and baseline antithrombin activity levels were 77 ± 12 U/dL.					
31232851	9	13	theme	activity	1424:1431	arg1	levels					1433:1438	Antithrombin activity levels	1411:1438	Antithrombin activity levels	1411:1438	Antithrombin activity levels were drawn a median 19.9 hours (interquartile range, 8.8-41.6 hr) after antithrombin dose.					
31232851	3	14	theme	care	379:382	arg1	hospital					395:402	Large tertiary care children's hospital	364:402	Large tertiary care children's hospital Subject inclusion criteria	364:429	SETTING Large tertiary care children's hospital Subject inclusion criteria consisted of less than 19 years old.					
31232851	10	15	theme	error	1562:1566	arg1	model					1568:1572	A one-compartment proportional error model	1531:1572	A one-compartment proportional error model	1531:1572	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	1	16	theme	pediatric	150:158	arg1	patients					160:167	pediatric patients	150:167	pediatric patients undergoing ventricular assist device therapy	150:212	OBJECTIVES Describe the pharmacokinetics of antithrombin in pediatric patients undergoing ventricular assist device therapy and provide dosing recommendations for antithrombin in this population.					
31232851	3	17	theme	Large	364:368	arg1	hospital					395:402	Large tertiary care children's hospital	364:402	Large tertiary care children's hospital Subject inclusion criteria	364:429	SETTING Large tertiary care children's hospital Subject inclusion criteria consisted of less than 19 years old.					
31232851	2	18	theme	pharmacokinetic	320:334	arg1	study					336:340	A retrospective population pharmacokinetic study	293:340	DESIGN A retrospective population pharmacokinetic study	286:340	DESIGN A retrospective population pharmacokinetic study was designed.					
31232851	6	19	theme	years	996:1000	arg1	range					1017:1021	5.8 years [interquartile range	992:1021	5.8 years [interquartile range	992:1021	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	19	theme	years	996:1000	arg1	age					987:989	median age	980:989	median age	980:989	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	3	20	theme	Subject	404:410	arg1	criteria					422:429	Large tertiary care children's hospital Subject inclusion criteria	364:429	Large tertiary care children's hospital Subject inclusion criteria	364:429	SETTING Large tertiary care children's hospital Subject inclusion criteria consisted of less than 19 years old.					
31232851	14	21	theme	heparin	1977:1983	arg1	dose					1985:1988	Unfractionated heparin dose	1962:1988	Unfractionated heparin dose	1962:1988	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	2	22	theme	retrospective	295:307	arg1	study					336:340	A retrospective population pharmacokinetic study	293:340	DESIGN A retrospective population pharmacokinetic study	286:340	DESIGN A retrospective population pharmacokinetic study was designed.					
31232851	0	23	from	Pharmacokinetics	24:39	arg1	Ventricular					54:64	Pediatric Ventricular	44:64	Pediatric Ventricular	44:64	Antithrombin Population Pharmacokinetics in Pediatric Ventricular Assist Device Patients.					
31232851	4	24	theme	antithrombin	725:736	arg1	level					747:751	a postdose antithrombin activity level	714:751	a postdose antithrombin activity level from January 1, 2011, to June 30, 2017	714:790	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	11	25	theme	baseline	1715:1722	arg1	heparin					1703:1709	Unfractionated heparin	1688:1709	Unfractionated heparin	1688:1709	Unfractionated heparin and baseline antithrombin were identified as significant covariates.					
31232851	11	25	theme	baseline	1715:1722	arg1	covariates					1768:1777	significant covariates	1756:1777	significant covariates	1756:1777	Unfractionated heparin and baseline antithrombin were identified as significant covariates.					
31232851	11	25	theme	baseline	1715:1722	arg1	antithrombin					1724:1735	baseline antithrombin	1715:1735	baseline antithrombin	1715:1735	Unfractionated heparin and baseline antithrombin were identified as significant covariates.					
31232851	6	26	theme	median	980:985	arg1	range					1017:1021	5.8 years [interquartile range	992:1021	5.8 years [interquartile range	992:1021	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	26	theme	median	980:985	arg1	age					987:989	median age	980:989	median age	980:989	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	26	theme	median	980:985	arg1	criteria					970:977	met study criteria	960:977	41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr])	948:1035	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	4	27	dep	assist	627:632	arg1	HeartWare					572:580	HeartWare	572:580	HeartWare	572:580	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	4	27	dep	assist	627:632	arg1	MA					595:596	MA	595:596	MA	595:596	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	4	27	dep	assist	627:632	arg1	Framingham					583:592	Framingham	583:592	Framingham	583:592	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	14	28	theme	baseline	1994:2001	arg1	level					2025:2029	baseline antithrombin activity level	1994:2029	baseline antithrombin activity level	1994:2029	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	4	29	theme	HeartWare	535:543	arg1	device					564:569	a HeartWare ventricular assist device	533:569	a HeartWare ventricular assist device	533:569	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	14	30	theme	pediatric	2080:2088	arg1	patients					2116:2123	pediatric ventricular assist device patients	2080:2123	pediatric ventricular assist device patients	2080:2123	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	1	31	dep	device	199:204	arg1	assist					192:197	assist	192:197	assist	192:197	OBJECTIVES Describe the pharmacokinetics of antithrombin in pediatric patients undergoing ventricular assist device therapy and provide dosing recommendations for antithrombin in this population.					
31232851	4	32	with	therapy	520:526	arg1	device					564:569	a HeartWare ventricular assist device	533:569	a HeartWare ventricular assist device	533:569	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	0	33	theme	Antithrombin	0:11	arg1	Pharmacokinetics					24:39	Antithrombin Population Pharmacokinetics	0:39	Antithrombin Population Pharmacokinetics in Pediatric Ventricular	0:64	Antithrombin Population Pharmacokinetics in Pediatric Ventricular Assist Device Patients.					
31232851	4	34	dep	device	564:569	arg1	assist					557:562	assist	557:562	assist	557:562	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	14	35	theme	Unfractionated	1962:1975	arg1	dose					1985:1988	Unfractionated heparin dose	1962:1988	Unfractionated heparin dose	1962:1988	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	7	36	theme	29 ± 14	1260:1266	arg1	U/kg/hr					1268:1274	29 ± 14 U/kg/hr	1260:1274	29 ± 14 U/kg/hr	1260:1274	All patients received unfractionated heparin continuous infusion at a mean ± SD dose of 29 ± 14 U/kg/hr.					
31232851	4	37	theme	Berlin	602:607	arg1	ventricular					615:625	Berlin EXCOR ventricular	602:625	Berlin EXCOR ventricular	602:625	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	10	38	theme	fat-free	1620:1627	arg1	mass					1629:1632	fat-free mass	1620:1632	fat-free mass	1620:1632	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	1	39	theme	antithrombin	134:145	arg1	pharmacokinetics					114:129	the pharmacokinetics	110:129	the pharmacokinetics of antithrombin in pediatric patients undergoing ventricular assist device therapy	110:212	OBJECTIVES Describe the pharmacokinetics of antithrombin in pediatric patients undergoing ventricular assist device therapy and provide dosing recommendations for antithrombin in this population.					
31232851	1	40	theme	ventricular	180:190	arg1	device					199:204	ventricular assist device	180:204	ventricular assist device therapy	180:212	OBJECTIVES Describe the pharmacokinetics of antithrombin in pediatric patients undergoing ventricular assist device therapy and provide dosing recommendations for antithrombin in this population.					
31232851	0	41	theme	Pediatric	44:52	arg1	Ventricular					54:64	Pediatric Ventricular	44:64	Pediatric Ventricular	44:64	Antithrombin Population Pharmacokinetics in Pediatric Ventricular Assist Device Patients.					
31232851	12	42	theme	simulated	1817:1825	arg1	hours					1848:1852	a simulated half-life 13.2 ± 6.6 hours	1815:1852	a simulated half-life 13.2 ± 6.6 hours	1815:1852	A 50 U/kg dose of antithrombin had a simulated half-life 13.2 ± 6.6 hours.					
31232851	7	43	theme	±	1247:1247	arg1	dose					1252:1255	a mean ± SD dose	1240:1255	a mean ± SD dose of 29 ± 14 U/kg/hr	1240:1274	All patients received unfractionated heparin continuous infusion at a mean ± SD dose of 29 ± 14 U/kg/hr.					
31232851	10	44	theme	better	1646:1651	arg1	fit					1659:1661	a better model fit	1644:1661	a better model fit than actual body weight	1644:1685	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	5	45	theme	Population	807:816	arg1	analysis					834:841	Population pharmacokinetic analysis	807:841	Population pharmacokinetic analysis	807:841	INTERVENTIONS Population pharmacokinetic analysis and simulation using NONMEM v.7.4 (Icon, PLC, Dublin, Ireland).					
31232851	12	46	theme	U/kg	1785:1788	arg1	dose					1790:1793	A 50 U/kg dose	1780:1793	A 50 U/kg dose of antithrombin	1780:1809	A 50 U/kg dose of antithrombin had a simulated half-life 13.2 ± 6.6 hours.					
31232851	6	47	theme	pulsatile	1075:1083	arg1	EXCOR					1092:1096	the pulsatile Berlin EXCOR pediatric ventricular	1071:1118	the pulsatile Berlin EXCOR pediatric ventricular	1071:1118	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	5	48	dep	v.7.4	871:875	arg1	Ireland					897:903	Ireland	897:903	Ireland	897:903	INTERVENTIONS Population pharmacokinetic analysis and simulation using NONMEM v.7.4 (Icon, PLC, Dublin, Ireland).					
31232851	5	48	dep	v.7.4	871:875	arg1	PLC					884:886	PLC	884:886	PLC	884:886	INTERVENTIONS Population pharmacokinetic analysis and simulation using NONMEM v.7.4 (Icon, PLC, Dublin, Ireland).					
31232851	5	48	dep	v.7.4	871:875	arg1	Icon					878:881	Icon	878:881	Icon	878:881	INTERVENTIONS Population pharmacokinetic analysis and simulation using NONMEM v.7.4 (Icon, PLC, Dublin, Ireland).					
31232851	8	49	theme	antithrombin	1363:1374	arg1	levels					1385:1390	baseline antithrombin activity levels	1354:1390	baseline antithrombin activity levels	1354:1390	A total of 181 antithrombin doses (44.1 ± 24.6 U/kg/dose) were included, and baseline antithrombin activity levels were 77 ± 12 U/dL.					
31232851	10	50	theme	actual	1668:1673	arg1	weight					1680:1685	actual body weight	1668:1685	actual body weight	1668:1685	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	1	51	from	pharmacokinetics	114:129	arg1	patients					160:167	pediatric patients	150:167	pediatric patients undergoing ventricular assist device therapy	150:212	OBJECTIVES Describe the pharmacokinetics of antithrombin in pediatric patients undergoing ventricular assist device therapy and provide dosing recommendations for antithrombin in this population.					
31232851	6	52	theme	MAIN	924:927	arg1	RESULTS					929:935	MAIN RESULTS	924:935	MAIN RESULTS	924:935	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	7	53	theme	heparin	1209:1215	arg1	infusion					1228:1235	unfractionated heparin continuous infusion	1194:1235	unfractionated heparin continuous infusion	1194:1235	All patients received unfractionated heparin continuous infusion at a mean ± SD dose of 29 ± 14 U/kg/hr.					
31232851	12	54	contain	had	1811:1813	arg1	dose					1790:1793	A 50 U/kg dose	1780:1793	A 50 U/kg dose of antithrombin	1780:1809	A 50 U/kg dose of antithrombin had a simulated half-life 13.2 ± 6.6 hours.					
31232851	12	54	contain	had	1811:1813	arg2	hours					1848:1852	a simulated half-life 13.2 ± 6.6 hours	1815:1852	a simulated half-life 13.2 ± 6.6 hours	1815:1852	A 50 U/kg dose of antithrombin had a simulated half-life 13.2 ± 6.6 hours.					
31232851	4	55	theme	PATIENTS	468:475	arg1	Subjects					477:484	PATIENTS Subjects	468:484	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device	468:569	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	13	56	theme	ventricular	1926:1936	arg1	device					1945:1950	ventricular assist device	1926:1950	pediatric ventricular assist device patients	1916:1959	CONCLUSIONS Antithrombin should be dosed on fat-free mass in pediatric ventricular assist device patients.					
31232851	5	57	dep	analysis	834:841	arg1	INTERVENTIONS					793:805	INTERVENTIONS	793:805	INTERVENTIONS	793:805	INTERVENTIONS Population pharmacokinetic analysis and simulation using NONMEM v.7.4 (Icon, PLC, Dublin, Ireland).					
31232851	13	58	theme	device	1945:1950	arg1	patients					1952:1959	pediatric ventricular assist device patients	1916:1959	pediatric ventricular assist device patients	1916:1959	CONCLUSIONS Antithrombin should be dosed on fat-free mass in pediatric ventricular assist device patients.					
31232851	6	59	with	therapy	1058:1064	arg1	EXCOR					1092:1096	the pulsatile Berlin EXCOR pediatric ventricular	1071:1118	the pulsatile Berlin EXCOR pediatric ventricular	1071:1118	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	60	dep	EXCOR	1092:1096	arg1	ventricular					1108:1118	pediatric ventricular	1098:1118	the pulsatile Berlin EXCOR pediatric ventricular	1071:1118	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	10	61	theme	proportional	1549:1560	arg1	model					1568:1572	A one-compartment proportional error model	1531:1572	A one-compartment proportional error model	1531:1572	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	13	62	theme	fat-free	1899:1906	arg1	mass					1908:1911	fat-free mass	1899:1911	fat-free mass in pediatric ventricular assist device patients	1899:1959	CONCLUSIONS Antithrombin should be dosed on fat-free mass in pediatric ventricular assist device patients.					
31232851	1	63	theme	dosing	226:231	arg1	recommendations					233:247	dosing recommendations	226:247	dosing recommendations for antithrombin in this population	226:283	OBJECTIVES Describe the pharmacokinetics of antithrombin in pediatric patients undergoing ventricular assist device therapy and provide dosing recommendations for antithrombin in this population.					
31232851	6	64	theme	study	964:968	arg1	criteria					970:977	met study criteria	960:977	41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr])	948:1035	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	64	theme	study	964:968	arg1	age					987:989	median age	980:989	median age	980:989	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	11	65	theme	significant	1756:1766	arg1	heparin					1703:1709	Unfractionated heparin	1688:1709	Unfractionated heparin	1688:1709	Unfractionated heparin and baseline antithrombin were identified as significant covariates.					
31232851	11	65	theme	significant	1756:1766	arg1	covariates					1768:1777	significant covariates	1756:1777	significant covariates	1756:1777	Unfractionated heparin and baseline antithrombin were identified as significant covariates.					
31232851	11	65	theme	significant	1756:1766	arg1	antithrombin					1724:1735	baseline antithrombin	1715:1735	baseline antithrombin	1715:1735	Unfractionated heparin and baseline antithrombin were identified as significant covariates.					
31232851	6	66	theme	patients	951:958	arg1	total					939:943	A total	937:943	A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr])	937:1035	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	66	theme	patients	951:958	arg1	patients					951:958	41 patients	948:958	41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr])	948:1035	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	66	theme	patients	951:958	arg1	%					1046:1046	53.7%	1042:1046	53.7%	1042:1046	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	9	67	theme	Antithrombin	1411:1422	arg1	levels					1433:1438	Antithrombin activity levels	1411:1438	Antithrombin activity levels	1411:1438	Antithrombin activity levels were drawn a median 19.9 hours (interquartile range, 8.8-41.6 hr) after antithrombin dose.					
31232851	2	68	dep	DESIGN	286:291	arg1	study					336:340	A retrospective population pharmacokinetic study	293:340	DESIGN A retrospective population pharmacokinetic study	286:340	DESIGN A retrospective population pharmacokinetic study was designed.					
31232851	13	69	from	mass	1908:1911	arg1	patients					1952:1959	pediatric ventricular assist device patients	1916:1959	pediatric ventricular assist device patients	1916:1959	CONCLUSIONS Antithrombin should be dosed on fat-free mass in pediatric ventricular assist device patients.					
31232851	6	70	dep	patients	951:958	arg1	criteria					970:977	met study criteria	960:977	41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr])	948:1035	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	70	dep	patients	951:958	arg1	age					987:989	median age	980:989	median age	980:989	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	4	71	theme	activity	738:745	arg1	level					747:751	a postdose antithrombin activity level	714:751	a postdose antithrombin activity level from January 1, 2011, to June 30, 2017	714:790	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	13	72	dep	device	1945:1950	arg1	assist					1938:1943	assist	1938:1943	assist	1938:1943	CONCLUSIONS Antithrombin should be dosed on fat-free mass in pediatric ventricular assist device patients.					
31232851	6	73	theme	[interquartile	1002:1015	arg1	range					1017:1021	5.8 years [interquartile range	992:1021	5.8 years [interquartile range	992:1021	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	6	73	theme	[interquartile	1002:1015	arg1	age					987:989	median age	980:989	median age	980:989	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	3	74	theme	tertiary	370:377	arg1	hospital					395:402	Large tertiary care children's hospital	364:402	Large tertiary care children's hospital Subject inclusion criteria	364:429	SETTING Large tertiary care children's hospital Subject inclusion criteria consisted of less than 19 years old.					
31232851	4	75	theme	old	505:507	arg1	Subjects					477:484	PATIENTS Subjects	468:484	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device	468:569	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	14	76	theme	antithrombin	2003:2014	arg1	level					2025:2029	baseline antithrombin activity level	1994:2029	baseline antithrombin activity level	1994:2029	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	11	77	theme	Unfractionated	1688:1701	arg1	covariates					1768:1777	significant covariates	1756:1777	significant covariates	1756:1777	Unfractionated heparin and baseline antithrombin were identified as significant covariates.					
31232851	11	77	theme	Unfractionated	1688:1701	arg1	heparin					1703:1709	Unfractionated heparin	1688:1709	Unfractionated heparin	1688:1709	Unfractionated heparin and baseline antithrombin were identified as significant covariates.					
31232851	11	77	theme	Unfractionated	1688:1701	arg1	antithrombin					1724:1735	baseline antithrombin	1715:1735	baseline antithrombin	1715:1735	Unfractionated heparin and baseline antithrombin were identified as significant covariates.					
31232851	2	78	theme	population	309:318	arg1	study					336:340	A retrospective population pharmacokinetic study	293:340	DESIGN A retrospective population pharmacokinetic study	286:340	DESIGN A retrospective population pharmacokinetic study was designed.					
31232851	1	79	from	recommendations	233:247	arg1	population					274:283	this population	269:283	this population	269:283	OBJECTIVES Describe the pharmacokinetics of antithrombin in pediatric patients undergoing ventricular assist device therapy and provide dosing recommendations for antithrombin in this population.					
31232851	9	80	theme	median	1453:1458	arg1	hours					1465:1469	a median 19.9 hours	1451:1469	a median 19.9 hours (interquartile range, 8.8-41.6 hr)	1451:1504	Antithrombin activity levels were drawn a median 19.9 hours (interquartile range, 8.8-41.6 hr) after antithrombin dose.					
31232851	3	81	theme	inclusion	412:420	arg1	criteria					422:429	Large tertiary care children's hospital Subject inclusion criteria	364:429	Large tertiary care children's hospital Subject inclusion criteria	364:429	SETTING Large tertiary care children's hospital Subject inclusion criteria consisted of less than 19 years old.					
31232851	1	82	theme	device	199:204	arg1	therapy					206:212	ventricular assist device therapy	180:212	ventricular assist device therapy	180:212	OBJECTIVES Describe the pharmacokinetics of antithrombin in pediatric patients undergoing ventricular assist device therapy and provide dosing recommendations for antithrombin in this population.					
31232851	3	83	theme	hospital	395:402	arg1	criteria					422:429	Large tertiary care children's hospital Subject inclusion criteria	364:429	Large tertiary care children's hospital Subject inclusion criteria	364:429	SETTING Large tertiary care children's hospital Subject inclusion criteria consisted of less than 19 years old.					
31232851	6	84	dep	age	987:989	arg1	yr					1032:1033	1.6-9.9 yr	1024:1033	1.6-9.9 yr	1024:1033	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	4	85	theme	postdose	716:723	arg1	level					747:751	a postdose antithrombin activity level	714:751	a postdose antithrombin activity level from January 1, 2011, to June 30, 2017	714:790	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	9	86	theme	interquartile	1472:1484	arg1	8.8-41.6 hr					1493:1503	8.8-41.6 hr	1493:1503	8.8-41.6 hr	1493:1503	Antithrombin activity levels were drawn a median 19.9 hours (interquartile range, 8.8-41.6 hr) after antithrombin dose.					
31232851	9	86	theme	interquartile	1472:1484	arg1	range					1486:1490	interquartile range	1472:1490	interquartile range	1472:1490	Antithrombin activity levels were drawn a median 19.9 hours (interquartile range, 8.8-41.6 hr) after antithrombin dose.					
31232851	4	87	theme	ventricular	545:555	arg1	device					564:569	a HeartWare ventricular assist device	533:569	a HeartWare ventricular assist device	533:569	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	14	88	theme	device	2109:2114	arg1	patients					2116:2123	pediatric ventricular assist device patients	2080:2123	pediatric ventricular assist device patients	2080:2123	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	7	89	theme	U/kg/hr	1268:1274	arg1	dose					1252:1255	a mean ± SD dose	1240:1255	a mean ± SD dose of 29 ± 14 U/kg/hr	1240:1274	All patients received unfractionated heparin continuous infusion at a mean ± SD dose of 29 ± 14 U/kg/hr.					
31232851	14	90	theme	ventricular	2090:2100	arg1	patients					2116:2123	pediatric ventricular assist device patients	2080:2123	pediatric ventricular assist device patients	2080:2123	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	0	91	theme	Population	13:22	arg1	Pharmacokinetics					24:39	Antithrombin Population Pharmacokinetics	0:39	Antithrombin Population Pharmacokinetics in Pediatric Ventricular	0:64	Antithrombin Population Pharmacokinetics in Pediatric Ventricular Assist Device Patients.					
31232851	4	92	dep	January	758:764	arg1	June					778:781	June	778:781	June	778:781	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	4	92	dep	January	758:764	arg1	to					775:776	to	775:776	to	775:776	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	3	93	theme	old	463:465	arg1	years					457:461	less than 19 years old	444:465	less than 19 years old	444:465	SETTING Large tertiary care children's hospital Subject inclusion criteria consisted of less than 19 years old.					
31232851	4	94	theme	EXCOR	609:613	arg1	ventricular					615:625	Berlin EXCOR ventricular	602:625	Berlin EXCOR ventricular	602:625	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	8	95	theme	antithrombin	1292:1303	arg1	doses					1305:1309	181 antithrombin doses	1288:1309	181 antithrombin doses (44.1 ± 24.6 U/kg/dose)	1288:1333	A total of 181 antithrombin doses (44.1 ± 24.6 U/kg/dose) were included, and baseline antithrombin activity levels were 77 ± 12 U/dL.					
31232851	8	95	theme	antithrombin	1292:1303	arg1	U/kg/dose					1324:1332	44.1 ± 24.6 U/kg/dose	1312:1332	44.1 ± 24.6 U/kg/dose	1312:1332	A total of 181 antithrombin doses (44.1 ± 24.6 U/kg/dose) were included, and baseline antithrombin activity levels were 77 ± 12 U/dL.					
31232851	9	96	theme	antithrombin	1512:1523	arg1	dose					1525:1528	antithrombin dose	1512:1528	antithrombin dose	1512:1528	Antithrombin activity levels were drawn a median 19.9 hours (interquartile range, 8.8-41.6 hr) after antithrombin dose.					
31232851	7	97	theme	SD	1249:1250	arg1	dose					1252:1255	a mean ± SD dose	1240:1255	a mean ± SD dose of 29 ± 14 U/kg/hr	1240:1274	All patients received unfractionated heparin continuous infusion at a mean ± SD dose of 29 ± 14 U/kg/hr.					
31232851	8	98	theme	doses	1305:1309	arg1	total					1279:1283	A total	1277:1283	A total of 181 antithrombin doses (44.1 ± 24.6 U/kg/dose)	1277:1333	A total of 181 antithrombin doses (44.1 ± 24.6 U/kg/dose) were included, and baseline antithrombin activity levels were 77 ± 12 U/dL.					
31232851	14	99	theme	dosing	2057:2062	arg1	antithrombin					2064:2075	dosing antithrombin	2057:2075	dosing antithrombin	2057:2075	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	12	100	theme	half-life	1827:1835	arg1	hours					1848:1852	a simulated half-life 13.2 ± 6.6 hours	1815:1852	a simulated half-life 13.2 ± 6.6 hours	1815:1852	A 50 U/kg dose of antithrombin had a simulated half-life 13.2 ± 6.6 hours.					
31232851	7	101	theme	mean	1242:1245	arg1	dose					1252:1255	a mean ± SD dose	1240:1255	a mean ± SD dose of 29 ± 14 U/kg/hr	1240:1274	All patients received unfractionated heparin continuous infusion at a mean ± SD dose of 29 ± 14 U/kg/hr.					
31232851	4	102	with	dose	688:691	arg1	level					747:751	a postdose antithrombin activity level	714:751	a postdose antithrombin activity level from January 1, 2011, to June 30, 2017	714:790	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	10	103	theme	allometric	1598:1607	arg1	scaling					1609:1615	allometric scaling	1598:1615	allometric scaling of fat-free mass	1598:1632	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	0	104	theme	Device	73:78	arg1	Patients					80:87	Device Patients	73:87	Device Patients	73:87	Antithrombin Population Pharmacokinetics in Pediatric Ventricular Assist Device Patients.					
31232851	6	105	theme	Berlin	1085:1090	arg1	EXCOR					1092:1096	the pulsatile Berlin EXCOR pediatric ventricular	1071:1118	the pulsatile Berlin EXCOR pediatric ventricular	1071:1118	MEASUREMENTS AND MAIN RESULTS A total of 41 patients met study criteria (median age, 5.8 years [interquartile range, 1.6-9.9 yr]), and 53.7% underwent therapy with the pulsatile Berlin EXCOR pediatric ventricular assist device (Berlin Heart GmbH, Berlin, Germany).					
31232851	4	106	from	January	758:764	arg1	level					747:751	a postdose antithrombin activity level	714:751	a postdose antithrombin activity level from January 1, 2011, to June 30, 2017	714:790	PATIENTS Subjects less than 19 years old undergoing therapy with a HeartWare ventricular assist device (HeartWare, Framingham, MA) or Berlin EXCOR ventricular assist device (Berlin GmbH, Berlin, Germany), who received a dose of antithrombin with a postdose antithrombin activity level from January 1, 2011, to June 30, 2017.					
31232851	12	107	theme	antithrombin	1798:1809	arg1	dose					1790:1793	A 50 U/kg dose	1780:1793	A 50 U/kg dose of antithrombin	1780:1809	A 50 U/kg dose of antithrombin had a simulated half-life 13.2 ± 6.6 hours.					
31232851	7	108	theme	continuous	1217:1226	arg1	infusion					1228:1235	unfractionated heparin continuous infusion	1194:1235	unfractionated heparin continuous infusion	1194:1235	All patients received unfractionated heparin continuous infusion at a mean ± SD dose of 29 ± 14 U/kg/hr.					
31232851	14	109	theme	activity	2016:2023	arg1	level					2025:2029	baseline antithrombin activity level	1994:2029	baseline antithrombin activity level	1994:2029	Unfractionated heparin dose and baseline antithrombin activity level should be considered when dosing antithrombin in pediatric ventricular assist device patients.					
31232851	10	110	theme	mass	1629:1632	arg1	scaling					1609:1615	allometric scaling	1598:1615	allometric scaling of fat-free mass	1598:1632	A one-compartment proportional error model best fit the data, with allometric scaling of fat-free mass providing a better model fit than actual body weight.					
31232851	7	111	theme	unfractionated	1194:1207	arg1	heparin					1209:1215	unfractionated heparin	1194:1215	unfractionated heparin continuous infusion	1194:1235	All patients received unfractionated heparin continuous infusion at a mean ± SD dose of 29 ± 14 U/kg/hr.					
31232851	8	112	theme	baseline	1354:1361	arg1	levels					1385:1390	baseline antithrombin activity levels	1354:1390	baseline antithrombin activity levels	1354:1390	A total of 181 antithrombin doses (44.1 ± 24.6 U/kg/dose) were included, and baseline antithrombin activity levels were 77 ± 12 U/dL.					
31232851	5	113	theme	pharmacokinetic	818:832	arg1	analysis					834:841	Population pharmacokinetic analysis	807:841	Population pharmacokinetic analysis	807:841	INTERVENTIONS Population pharmacokinetic analysis and simulation using NONMEM v.7.4 (Icon, PLC, Dublin, Ireland).					
29307533	8	0	theme	proteins	1496:1503	arg1	coefficients					1480:1491	The partition coefficients	1466:1491	The partition coefficients of proteins in PEG-600-choline chloride ATPS	1466:1536	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	4	1	theme	PEG-600	1001:1007	arg1	concentrations					943:956	The concentrations	939:956	The concentrations of the aforementioned polymers, as well as PEG-600 and PEG-1000 required for phase separation in mixtures with choline chloride reported in the literature	939:1111	The concentrations of the aforementioned polymers, as well as PEG-600 and PEG-1000 required for phase separation in mixtures with choline chloride reported in the literature are analyzed.					
29307533	3	2	theme	Solvent	638:644	arg1	properties					646:655	Solvent properties	638:655	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride	638:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	6	3	theme	choline	1227:1233	arg1	chloride					1235:1242	choline chloride	1227:1242	choline chloride	1227:1242	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	9	4	theme	direct	1851:1856	arg1	interactions					1858:1869	direct interactions	1851:1869	direct interactions with choline chloride	1851:1891	Analysis of partition coefficients in the two ATPS were compared to those reported in Dextran-PEG ATPS, and proteins likely engaged in direct interactions with choline chloride were identified.					
29307533	7	5	theme	proteins	1427:1434	arg1	behavior					1410:1417	Partition behavior	1400:1417	Partition behavior of nine proteins	1400:1434	Partition behavior of nine proteins was examined in both systems.					
29307533	2	6	theme	phosphate	605:613	arg1	NaPB					623:626	NaPB	623:626	NaPB	623:626	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	2	6	theme	phosphate	605:613	arg1	pH					630:631	pH 7.4	630:635	pH 7.4	630:635	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	2	6	theme	phosphate	605:613	arg1	buffer					615:620	0.01 M sodium phosphate buffer	591:620	0.01 M sodium phosphate buffer (NaPB)	591:627	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	8	7	theme	phases	1689:1694	arg1	composition					1659:1669	the composition	1655:1669	the composition of the coexisting phases in the former ATPS	1655:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	2	8	theme	0.01 M	591:596	arg1	NaPB					623:626	NaPB	623:626	NaPB	623:626	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	2	8	theme	0.01 M	591:596	arg1	pH					630:631	pH 7.4	630:635	pH 7.4	630:635	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	2	8	theme	0.01 M	591:596	arg1	buffer					615:620	0.01 M sodium phosphate buffer	591:620	0.01 M sodium phosphate buffer (NaPB)	591:627	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	1	9	theme	solvent	449:455	arg1	properties					457:466	hydrophobic, electrostatic and other solvent properties	412:466	hydrophobic, electrostatic and other solvent properties of the phases	412:480	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	10	theme	aqueous	212:218	arg1	system					230:235	a new aqueous two-phase system	206:235	a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4,	206:392	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	10	theme	aqueous	212:218	arg1	ATPS					238:241	ATPS	238:241	ATPS	238:241	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	3	11	theme	bond	703:706	arg1	donor					708:712	hydrogen bond donor	694:712	hydrogen bond donor acidity	694:720	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	6	12	theme	bond	1285:1288	arg1	donor					1290:1294	water hydrogen bond donor	1270:1294	water hydrogen bond donor	1270:1294	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	6	13	from	concentrations	1384:1397	arg1	related					1257:1263	related	1257:1263	related	1257:1263	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	1	14	theme	system	230:235	arg1	diagram					195:201	The phase diagram	185:201	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4,	185:392	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	4	15	theme	choline	1069:1075	arg1	chloride					1077:1084	choline chloride	1069:1084	choline chloride reported in the literature	1069:1111	The concentrations of the aforementioned polymers, as well as PEG-600 and PEG-1000 required for phase separation in mixtures with choline chloride reported in the literature are analyzed.					
29307533	3	16	from	properties	646:655	arg1	solutions					771:779	aqueous solutions	763:779	aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride	763:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	3	17	theme	bond	736:739	arg1	basicity					750:757	hydrogen bond acceptor basicity	727:757	hydrogen bond acceptor basicity	727:757	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	0	18	theme	two-phase	166:174	arg1	systems					176:182	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	systems	176:182	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	6	19	dep	donor	1290:1294	arg1	acidity					1296:1302	acidity	1296:1302	acidity	1296:1302	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	0	20	from	partitioning	50:61	arg1	N-oxide					113:119	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	N-oxide	113:119	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	0	20	from	partitioning	50:61	arg1	systems					176:182	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	systems	176:182	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	3	21	from	water	660:664	arg1	solutions					771:779	aqueous solutions	763:779	aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride	763:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	8	22	from	ATPS	1533:1536	arg1	coefficients					1480:1491	The partition coefficients	1466:1491	The partition coefficients of proteins in PEG-600-choline chloride ATPS	1466:1536	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	5	23	theme	polymers	1166:1173	arg1	concentrations					1148:1161	the concentrations	1144:1161	the concentrations of polymers	1144:1173	It is found that the concentrations of polymers needed for phase separation in mixtures with 35%wt.					
29307533	3	24	theme	dimethyl	840:847	arg1	PEGDME-250					861:870	PEGDME-250	861:870	PEGDME-250	861:870	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	3	24	theme	dimethyl	840:847	arg1	ether					849:853	polyethylene glycol dimethyl ether -250	820:858	polyethylene glycol dimethyl ether -250 (PEGDME-250)	820:871	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	3	25	theme	polypropylene	784:796	arg1	solutions					771:779	aqueous solutions	763:779	aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride	763:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	0	26	theme	aqueous	66:72	arg1	N-oxide					113:119	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	N-oxide	113:119	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	5	27	with	mixtures	1206:1213	arg1	wt					1223:1224	35%wt	1220:1224	35%wt	1220:1224	It is found that the concentrations of polymers needed for phase separation in mixtures with 35%wt.					
29307533	3	28	theme	polyethylene	820:831	arg1	PEGDME-250					861:870	PEGDME-250	861:870	PEGDME-250	861:870	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	3	28	theme	polyethylene	820:831	arg1	ether					849:853	polyethylene glycol dimethyl ether -250	820:858	polyethylene glycol dimethyl ether -250 (PEGDME-250)	820:871	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	2	29	theme	same	505:508	arg1	properties					510:519	The same properties	501:519	The same properties	501:519	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	5	30	theme	%	1222:1222	arg1	wt					1223:1224	35%wt	1220:1224	35%wt	1220:1224	It is found that the concentrations of polymers needed for phase separation in mixtures with 35%wt.					
29307533	0	31	theme	glycol-600-trimethylamine	87:111	arg1	N-oxide					113:119	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	N-oxide	113:119	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	1	32	theme	sodium	354:359	arg1	NaPB					379:382	NaPB	379:382	NaPB	379:382	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	32	theme	sodium	354:359	arg1	pH					386:387	pH 7.4	386:391	pH 7.4	386:391	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	32	theme	sodium	354:359	arg1	buffer					371:376	0.01 M sodium phosphate buffer	347:376	0.01 M sodium phosphate buffer (NaPB)	347:383	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	0	33	from	properties	26:35	arg1	N-oxide					113:119	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	N-oxide	113:119	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	0	33	from	properties	26:35	arg1	systems					176:182	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	systems	176:182	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	8	34	from	composition	1659:1669	arg1	ATPS					1710:1713	the former ATPS	1699:1713	the former ATPS	1699:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	0	35	theme	Phase	0:4	arg1	equilibria					6:15	Phase equilibria	0:15	Phase equilibria	0:15	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	3	36	theme	choline	878:884	arg1	chloride					886:893	choline chloride	878:893	choline chloride	878:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	8	37	theme	PEG-600-choline	1508:1522	arg1	ATPS					1533:1536	PEG-600-choline chloride ATPS	1508:1536	PEG-600-choline chloride ATPS	1508:1536	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	3	38	from	solutions	771:779	arg1	properties					646:655	Solvent properties	638:655	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride	638:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	6	39	theme	given	1378:1382	arg1	concentrations					1384:1397	given concentrations	1378:1397	given concentrations	1378:1397	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	8	40	from	phases	1689:1694	arg1	ATPS					1710:1713	the former ATPS	1699:1713	the former ATPS	1699:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	8	41	from	proteins	1496:1503	arg1	ATPS					1533:1536	PEG-600-choline chloride ATPS	1508:1536	PEG-600-choline chloride ATPS	1508:1536	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	9	42	from	coefficients	1738:1749	arg1	ATPS					1762:1765	the two ATPS	1754:1765	the two ATPS	1754:1765	Analysis of partition coefficients in the two ATPS were compared to those reported in Dextran-PEG ATPS, and proteins likely engaged in direct interactions with choline chloride were identified.					
29307533	4	43	theme	polymers	980:987	arg1	concentrations					943:956	The concentrations	939:956	The concentrations of the aforementioned polymers, as well as PEG-600 and PEG-1000 required for phase separation in mixtures with choline chloride reported in the literature	939:1111	The concentrations of the aforementioned polymers, as well as PEG-600 and PEG-1000 required for phase separation in mixtures with choline chloride reported in the literature are analyzed.					
29307533	6	44	theme	individual	1346:1355	arg1	solutions					1365:1373	the individual polymer solutions	1342:1373	the individual polymer solutions	1342:1373	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	1	45	with	glycol	267:272	arg1	PEG-600					301:307	PEG-600	301:307	PEG-600	301:307	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	45	with	glycol	267:272	arg1	TMAO					338:341	TMAO	338:341	TMAO	338:341	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	45	with	glycol	267:272	arg1	N-oxide					329:335	trimethylamine N-oxide	314:335	trimethylamine N-oxide (TMAO)	314:342	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	45	with	glycol	267:272	arg1	weight					289:294	molecular weight 600	279:298	molecular weight 600 (PEG-600)	279:308	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	4	46	theme	phase	1035:1039	arg1	separation					1041:1050	phase separation	1035:1050	phase separation	1035:1050	The concentrations of the aforementioned polymers, as well as PEG-600 and PEG-1000 required for phase separation in mixtures with choline chloride reported in the literature are analyzed.					
29307533	8	47	from	ATPS	1710:1713	arg1	composition					1659:1669	the composition	1655:1669	the composition of the coexisting phases in the former ATPS	1655:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	4	48	from	concentrations	943:956	arg1	mixtures					1055:1062	mixtures	1055:1062	mixtures with choline chloride reported in the literature	1055:1111	The concentrations of the aforementioned polymers, as well as PEG-600 and PEG-1000 required for phase separation in mixtures with choline chloride reported in the literature are analyzed.					
29307533	4	49	with	mixtures	1055:1062	arg1	chloride					1077:1084	choline chloride	1069:1084	choline chloride reported in the literature	1069:1111	The concentrations of the aforementioned polymers, as well as PEG-600 and PEG-1000 required for phase separation in mixtures with choline chloride reported in the literature are analyzed.					
29307533	6	50	theme	polymer	1357:1363	arg1	solutions					1365:1373	the individual polymer solutions	1342:1373	the individual polymer solutions	1342:1373	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	3	51	theme	water	660:664	arg1	properties					646:655	Solvent properties	638:655	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride	638:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	2	52	theme	choline	571:577	arg1	chloride					579:586	choline chloride	571:586	choline chloride	571:586	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	1	53	theme	hydrophobic	412:422	arg1	properties					457:466	hydrophobic, electrostatic and other solvent properties	412:466	hydrophobic, electrostatic and other solvent properties of the phases	412:480	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	54	theme	molecular	279:287	arg1	PEG-600					301:307	PEG-600	301:307	PEG-600	301:307	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	54	theme	molecular	279:287	arg1	weight					289:294	molecular weight 600	279:298	molecular weight 600 (PEG-600)	279:308	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	7	55	theme	Partition	1400:1408	arg1	behavior					1410:1417	Partition behavior	1400:1417	Partition behavior of nine proteins	1400:1434	Partition behavior of nine proteins was examined in both systems.					
29307533	3	56	theme	hydrogen	694:701	arg1	donor					708:712	hydrogen bond donor	694:712	hydrogen bond donor acidity	694:720	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	9	57	theme	coefficients	1738:1749	arg1	Analysis					1716:1723	Analysis	1716:1723	Analysis of partition coefficients in the two ATPS	1716:1765	Analysis of partition coefficients in the two ATPS were compared to those reported in Dextran-PEG ATPS, and proteins likely engaged in direct interactions with choline chloride were identified.					
29307533	1	58	theme	electrostatic	425:437	arg1	properties					457:466	hydrophobic, electrostatic and other solvent properties	412:466	hydrophobic, electrostatic and other solvent properties of the phases	412:480	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	8	59	from	coefficients	1480:1491	arg1	ATPS					1533:1536	PEG-600-choline chloride ATPS	1508:1536	PEG-600-choline chloride ATPS	1508:1536	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	9	60	from	ATPS	1762:1765	arg1	Analysis					1716:1723	Analysis	1716:1723	Analysis of partition coefficients in the two ATPS	1716:1765	Analysis of partition coefficients in the two ATPS were compared to those reported in Dextran-PEG ATPS, and proteins likely engaged in direct interactions with choline chloride were identified.					
29307533	2	61	theme	sodium	598:603	arg1	NaPB					623:626	NaPB	623:626	NaPB	623:626	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	2	61	theme	sodium	598:603	arg1	pH					630:631	pH 7.4	630:635	pH 7.4	630:635	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	2	61	theme	sodium	598:603	arg1	buffer					615:620	0.01 M sodium phosphate buffer	591:620	0.01 M sodium phosphate buffer (NaPB)	591:627	The same properties are determined for the ATPS formed by PEG-600 and choline chloride in 0.01 M sodium phosphate buffer (NaPB), pH 7.4.					
29307533	9	62	theme	choline	1876:1882	arg1	chloride					1884:1891	choline chloride	1876:1891	choline chloride	1876:1891	Analysis of partition coefficients in the two ATPS were compared to those reported in Dextran-PEG ATPS, and proteins likely engaged in direct interactions with choline chloride were identified.					
29307533	9	63	theme	partition	1728:1736	arg1	coefficients					1738:1749	partition coefficients	1728:1749	partition coefficients in the two ATPS	1728:1765	Analysis of partition coefficients in the two ATPS were compared to those reported in Dextran-PEG ATPS, and proteins likely engaged in direct interactions with choline chloride were identified.					
29307533	1	64	theme	other	443:447	arg1	properties					457:466	hydrophobic, electrostatic and other solvent properties	412:466	hydrophobic, electrostatic and other solvent properties of the phases	412:480	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	8	65	theme	coexisting	1678:1687	arg1	phases					1689:1694	the coexisting phases	1674:1694	the coexisting phases in the former ATPS	1674:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	1	66	theme	new	208:210	arg1	system					230:235	a new aqueous two-phase system	206:235	a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4,	206:392	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	66	theme	new	208:210	arg1	ATPS					238:241	ATPS	238:241	ATPS	238:241	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	8	67	theme	partition	1470:1478	arg1	coefficients					1480:1491	The partition coefficients	1466:1491	The partition coefficients of proteins in PEG-600-choline chloride ATPS	1466:1536	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	0	68	theme	chloride	157:164	arg1	systems					176:182	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	systems	176:182	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	1	69	theme	two-phase	220:228	arg1	system					230:235	a new aqueous two-phase system	206:235	a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4,	206:392	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	69	theme	two-phase	220:228	arg1	ATPS					238:241	ATPS	238:241	ATPS	238:241	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	3	70	theme	donor	708:712	arg1	acidity					714:720	hydrogen bond donor acidity	694:720	hydrogen bond donor acidity	694:720	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	1	71	from	glycol	267:272	arg1	NaPB					379:382	NaPB	379:382	NaPB	379:382	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	71	from	glycol	267:272	arg1	pH					386:387	pH 7.4	386:391	pH 7.4	386:391	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	71	from	glycol	267:272	arg1	buffer					371:376	0.01 M sodium phosphate buffer	347:376	0.01 M sodium phosphate buffer (NaPB)	347:383	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	6	72	with	related	1257:1263	arg1	acceptor					1321:1328	hydrogen bond acceptor basicity	1307:1337	hydrogen bond acceptor basicity	1307:1337	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	6	72	with	related	1257:1263	arg1	donor					1290:1294	water hydrogen bond donor	1270:1294	water hydrogen bond donor	1270:1294	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	3	73	theme	acceptor	741:748	arg1	basicity					750:757	hydrogen bond acceptor basicity	727:757	hydrogen bond acceptor basicity	727:757	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	5	74	from	separation	1192:1201	arg1	mixtures					1206:1213	mixtures	1206:1213	mixtures with 35%wt	1206:1224	It is found that the concentrations of polymers needed for phase separation in mixtures with 35%wt.					
29307533	8	75	from	2	1592:1592	arg1	ATPS					1578:1581	PEG-600-TMAO ATPS	1565:1581	PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS	1565:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	3	76	theme	hydrogen	727:734	arg1	basicity					750:757	hydrogen bond acceptor basicity	727:757	hydrogen bond acceptor basicity	727:757	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	3	77	theme	aqueous	763:769	arg1	solutions					771:779	aqueous solutions	763:779	aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride	763:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	6	78	from	solutions	1365:1373	arg1	related					1257:1263	related	1257:1263	related	1257:1263	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	6	79	theme	hydrogen	1307:1314	arg1	acceptor					1321:1328	hydrogen bond acceptor basicity	1307:1337	hydrogen bond acceptor basicity	1307:1337	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	0	80	theme	polyethylene	74:85	arg1	N-oxide					113:119	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	N-oxide	113:119	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	8	81	dep	75-fold	1601:1607	arg1	ca.					1597:1599	ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS	1597:1713	ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS	1597:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	1	82	theme	phase	189:193	arg1	diagram					195:201	The phase diagram	185:201	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4,	185:392	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	3	83	theme	ether	849:853	arg1	solutions					771:779	aqueous solutions	763:779	aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride	763:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	0	84	theme	glycol-600-choline	138:155	arg1	systems					176:182	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	systems	176:182	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	0	85	from	equilibria	6:15	arg1	N-oxide					113:119	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	N-oxide	113:119	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	0	85	from	equilibria	6:15	arg1	systems					176:182	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	systems	176:182	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	5	86	theme	35	1220:1221	arg1	%					1222:1222	%	1222:1222	%	1222:1222	It is found that the concentrations of polymers needed for phase separation in mixtures with 35%wt.					
29307533	6	87	theme	bond	1316:1319	arg1	acceptor					1321:1328	hydrogen bond acceptor basicity	1307:1337	hydrogen bond acceptor basicity	1307:1337	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	1	88	theme	0.01 M	347:352	arg1	NaPB					379:382	NaPB	379:382	NaPB	379:382	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	88	theme	0.01 M	347:352	arg1	pH					386:387	pH 7.4	386:391	pH 7.4	386:391	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	88	theme	0.01 M	347:352	arg1	buffer					371:376	0.01 M sodium phosphate buffer	347:376	0.01 M sodium phosphate buffer (NaPB)	347:383	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	3	89	theme	glycol	833:838	arg1	PEGDME-250					861:870	PEGDME-250	861:870	PEGDME-250	861:870	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	3	89	theme	glycol	833:838	arg1	ether					849:853	polyethylene glycol dimethyl ether -250	820:858	polyethylene glycol dimethyl ether -250 (PEGDME-250)	820:871	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	0	90	theme	polyethylene	125:136	arg1	systems					176:182	aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems	66:182	systems	176:182	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	1	91	theme	phosphate	361:369	arg1	NaPB					379:382	NaPB	379:382	NaPB	379:382	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	91	theme	phosphate	361:369	arg1	pH					386:387	pH 7.4	386:391	pH 7.4	386:391	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	91	theme	phosphate	361:369	arg1	buffer					371:376	0.01 M sodium phosphate buffer	347:376	0.01 M sodium phosphate buffer (NaPB)	347:383	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	6	92	theme	hydrogen	1276:1283	arg1	donor					1290:1294	water hydrogen bond donor	1270:1294	water hydrogen bond donor	1270:1294	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	8	93	theme	chloride	1524:1531	arg1	ATPS					1533:1536	PEG-600-choline chloride ATPS	1508:1536	PEG-600-choline chloride ATPS	1508:1536	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	0	94	theme	solvent	18:24	arg1	properties					26:35	solvent properties	18:35	solvent properties	18:35	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	3	95	theme	chloride	886:893	arg1	solutions					771:779	aqueous solutions	763:779	aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride	763:893	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	9	96	theme	Dextran-PEG	1802:1812	arg1	ATPS					1814:1817	Dextran-PEG ATPS	1802:1817	Dextran-PEG ATPS	1802:1817	Analysis of partition coefficients in the two ATPS were compared to those reported in Dextran-PEG ATPS, and proteins likely engaged in direct interactions with choline chloride were identified.					
29307533	8	97	theme	former	1703:1708	arg1	ATPS					1710:1713	the former ATPS	1699:1713	the former ATPS	1699:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	3	98	dep	water	660:664	arg1	acidity					714:720	hydrogen bond donor acidity	694:720	hydrogen bond donor acidity	694:720	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	3	98	dep	water	660:664	arg1	dipolarity/polarizability					667:691	dipolarity/polarizability	667:691	dipolarity/polarizability	667:691	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	3	98	dep	water	660:664	arg1	basicity					750:757	hydrogen bond acceptor basicity	727:757	hydrogen bond acceptor basicity	727:757	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	0	99	theme	protein	42:48	arg1	partitioning					50:61	protein partitioning	42:61	protein partitioning	42:61	Phase equilibria, solvent properties, and protein partitioning in aqueous polyethylene glycol-600-trimethylamine N-oxide and polyethylene glycol-600-choline chloride two-phase systems.					
29307533	1	100	theme	trimethylamine	314:327	arg1	TMAO					338:341	TMAO	338:341	TMAO	338:341	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	1	100	theme	trimethylamine	314:327	arg1	N-oxide					329:335	trimethylamine N-oxide	314:335	trimethylamine N-oxide (TMAO)	314:342	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	3	101	theme	different	913:921	arg1	concentrations					923:936	different concentrations	913:936	different concentrations	913:936	Solvent properties of water (dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity) in aqueous solutions of polypropylene glycol-400 (PPG-400), polyethylene glycol dimethyl ether -250 (PEGDME-250), and choline chloride are determined at different concentrations.					
29307533	4	102	theme	aforementioned	965:978	arg1	polymers					980:987	the aforementioned polymers	961:987	the aforementioned polymers	961:987	The concentrations of the aforementioned polymers, as well as PEG-600 and PEG-1000 required for phase separation in mixtures with choline chloride reported in the literature are analyzed.					
29307533	8	103	theme	PEG-600-TMAO	1565:1576	arg1	ATPS					1578:1581	PEG-600-TMAO ATPS	1565:1581	PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS	1565:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	1	104	theme	phases	475:480	arg1	properties					457:466	hydrophobic, electrostatic and other solvent properties	412:466	hydrophobic, electrostatic and other solvent properties of the phases	412:480	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
29307533	6	105	from	related	1257:1263	arg1	solutions					1365:1373	the individual polymer solutions	1342:1373	the individual polymer solutions	1342:1373	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	6	106	theme	water	1270:1274	arg1	donor					1290:1294	water hydrogen bond donor	1270:1294	water hydrogen bond donor	1270:1294	choline chloride are linearly related with water hydrogen bond donor acidity or hydrogen bond acceptor basicity in the individual polymer solutions at given concentrations.					
29307533	9	107	with	interactions	1858:1869	arg1	chloride					1884:1891	choline chloride	1876:1891	choline chloride	1876:1891	Analysis of partition coefficients in the two ATPS were compared to those reported in Dextran-PEG ATPS, and proteins likely engaged in direct interactions with choline chloride were identified.					
29307533	8	108	theme	larger	1629:1634	arg1	difference					1636:1645	the larger difference	1625:1645	the larger difference between the composition of the coexisting phases in the former ATPS	1625:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	5	109	theme	phase	1186:1190	arg1	separation					1192:1201	phase separation	1186:1201	phase separation in mixtures with 35%wt	1186:1224	It is found that the concentrations of polymers needed for phase separation in mixtures with 35%wt.					
29307533	9	110	from	Analysis	1716:1723	arg1	ATPS					1762:1765	the two ATPS	1754:1765	the two ATPS	1754:1765	Analysis of partition coefficients in the two ATPS were compared to those reported in Dextran-PEG ATPS, and proteins likely engaged in direct interactions with choline chloride were identified.					
29307533	8	111	located	observed	1553:1560	arg1	ATPS					1578:1581	PEG-600-TMAO ATPS	1565:1581	PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS	1565:1713	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	8	111	located	observed	1553:1560	arg2	those					1547:1551	those	1547:1551	those	1547:1551	The partition coefficients of proteins in PEG-600-choline chloride ATPS exceeded those observed in PEG-600-TMAO ATPS from ca. 2 to ca. 75-fold possibly due to the larger difference between the composition of the coexisting phases in the former ATPS.					
29307533	1	112	theme	polyethylene	254:265	arg1	glycol					267:272	polyethylene glycol	254:272	polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4,	254:392	The phase diagram of a new aqueous two-phase system (ATPS) formed by polyethylene glycol with molecular weight 600 (PEG-600) and trimethylamine N-oxide (TMAO) in 0.01 M sodium phosphate buffer (NaPB), pH 7.4, is determined and hydrophobic, electrostatic and other solvent properties of the phases are characterized.					
30176798	8	0	theme	zooplankton-based	1109:1125	arg1	stages					1132:1137	zooplankton-based diet stages	1109:1137	zooplankton-based diet stages	1109:1137	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	9	1	located	detected	1244:1251	arg2	bacteria					1230:1237	thirteen potential cellulose-degrading bacteria	1191:1237	thirteen potential cellulose-degrading bacteria	1191:1237	Moreover, thirteen potential cellulose-degrading bacteria were detected in our study.					
30176798	9	1	located	detected	1244:1251	arg1	study					1260:1264	our study	1256:1264	our study	1256:1264	Moreover, thirteen potential cellulose-degrading bacteria were detected in our study.					
30176798	10	2	theme	microbiota	1508:1517	arg1	capacity					1492:1499	the metabolic capacity	1478:1499	the metabolic capacity of gut microbiota	1478:1517	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	11	3	theme	gut	1612:1614	arg1	microbiota					1616:1625	the gut microbiota	1608:1625	the gut microbiota	1608:1625	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	11	4	dep	CONCLUSIONS	1550:1560	arg1	provided					1573:1580	provided	1573:1580	provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time	1573:1702	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	11	4	dep	CONCLUSIONS	1550:1560	arg1	showed					1716:1721	showed	1716:1721	showed that the gut microbiota was strongly affected by feeding habit	1716:1784	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	3	5	theme	content	508:514	arg1	transition					452:461	transition	452:461	transition	452:461	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	3	5	theme	content	508:514	arg1	microbiota					468:477	gut microbiota	464:477	gut microbiota	464:477	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	3	5	theme	content	508:514	arg1	enzymes					493:499	digestive enzymes	483:499	digestive enzymes of gut content	483:514	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	3	5	theme	content	508:514	arg1	habits					445:450	feeding habits transition, gut microbiota and digestive enzymes of gut content	437:514	feeding habits transition, gut microbiota and digestive enzymes of gut content	437:514	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	8	6	theme	diet	1143:1146	arg1	stages					1159:1164	diet transition stages	1143:1164	diet transition stages	1143:1164	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	6	7	theme	bacterial	821:829	arg1	communities					831:841	gut bacterial communities	817:841	gut bacterial communities	817:841	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	11	8	theme	snout	1636:1640	arg1	bream					1642:1646	blunt snout bream	1630:1646	blunt snout bream	1630:1646	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	1	9	theme	Megalobrama	162:172	arg1	amblycephala					174:185	Megalobrama amblycephala	162:185	Megalobrama amblycephala	162:185	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	9	theme	Megalobrama	162:172	arg1	bream					155:159	The blunt snout bream	139:159	The blunt snout bream (Megalobrama amblycephala)	139:186	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	4	10	theme	MiSeq	629:633	arg1	platform					641:648	the Illumina MiSeq PE300 platform	616:648	the Illumina MiSeq PE300 platform	616:648	RESULTS In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform.					
30176798	10	11	theme	enzyme	1397:1402	arg1	activities					1404:1413	the digestive enzyme activities	1383:1413	the digestive enzyme activities of gut content	1383:1428	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	6	12	theme	compositions	914:925	arg1	result					885:890	the clustering result	870:890	the clustering result of intestinal content compositions	870:925	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	0	13	theme	habit	110:114	arg1	transition					116:125	feeding habit transition	102:125	feeding habit transition	102:125	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	9	14	theme	potential	1200:1208	arg1	bacteria					1230:1237	thirteen potential cellulose-degrading bacteria	1191:1237	thirteen potential cellulose-degrading bacteria	1191:1237	Moreover, thirteen potential cellulose-degrading bacteria were detected in our study.					
30176798	4	15	from	libraries	586:594	arg1	high-quality					548:559	high-quality	548:559	high-quality	548:559	RESULTS In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform.					
30176798	6	16	theme	intestinal	895:904	arg1	compositions					914:925	intestinal content compositions	895:925	intestinal content compositions	895:925	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	7	17	theme	bream	1010:1014	arg1	microbiota					984:993	the 'core' gut microbiota	969:993	the 'core' gut microbiota of blunt snout bream	969:1014	Proteobacteria and Firmicutes constitute the 'core' gut microbiota of blunt snout bream.					
30176798	7	18	theme	core	974:977	arg1	microbiota					984:993	the 'core' gut microbiota	969:993	the 'core' gut microbiota of blunt snout bream	969:1014	Proteobacteria and Firmicutes constitute the 'core' gut microbiota of blunt snout bream.					
30176798	12	19	from	habits	1900:1905	arg1	vertebrate					1910:1919	vertebrate	1910:1919	vertebrate	1910:1919	This work allows us to better understand the relationship among gut microbiota, nutrition metabolism and feeding habits in vertebrate.					
30176798	2	20	theme	Gut	334:336	arg1	microbiota					338:347	Gut microbiota	334:347	Gut microbiota	334:347	Gut microbiota has a vital role to host animal.					
30176798	0	21	theme	snout	56:60	arg1	bream					62:66	blunt snout bream	50:66	blunt snout bream (Megalobrama amblycephala)	50:93	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	0	21	theme	snout	56:60	arg1	amblycephala					81:92	amblycephala	81:92	amblycephala	81:92	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	1	22	theme	blunt	303:307	arg1	development					321:331	blunt snout bream development	303:331	blunt snout bream development	303:331	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	23	theme	blunt	143:147	arg1	amblycephala					174:185	Megalobrama amblycephala	162:185	Megalobrama amblycephala	162:185	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	23	theme	blunt	143:147	arg1	one					191:193	one	191:193	one	191:193	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	23	theme	blunt	143:147	arg1	fish					240:243	the most important commercial herbivorous fish	198:243	the most important commercial herbivorous fish in China	198:252	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	23	theme	blunt	143:147	arg1	bream					155:159	The blunt snout bream	139:159	The blunt snout bream (Megalobrama amblycephala)	139:186	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	0	24	from	profile	14:20	arg1	bream					62:66	blunt snout bream	50:66	blunt snout bream (Megalobrama amblycephala)	50:93	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	0	24	from	profile	14:20	arg1	amblycephala					81:92	amblycephala	81:92	amblycephala	81:92	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	1	25	theme	bream	315:319	arg1	development					321:331	blunt snout bream development	303:331	blunt snout bream development	303:331	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	10	26	theme	content	1422:1428	arg1	activities					1404:1413	the digestive enzyme activities	1383:1413	the digestive enzyme activities of gut content	1383:1428	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	10	26	theme	content	1422:1428	arg1	microbiota					1368:1377	the gut microbiota	1360:1377	the gut microbiota	1360:1377	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	11	27	theme	gut	1732:1734	arg1	microbiota					1736:1745	the gut microbiota	1728:1745	the gut microbiota	1728:1745	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	3	28	dep	habits	445:450	arg1	transition					452:461	transition	452:461	transition	452:461	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	3	28	dep	habits	445:450	arg1	microbiota					468:477	gut microbiota	464:477	gut microbiota	464:477	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	3	28	dep	habits	445:450	arg1	enzymes					493:499	digestive enzymes	483:499	digestive enzymes of gut content	483:514	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	3	28	dep	habits	445:450	arg1	habits					445:450	feeding habits transition, gut microbiota and digestive enzymes of gut content	437:514	feeding habits transition, gut microbiota and digestive enzymes of gut content	437:514	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	12	29	from	microbiota	1855:1864	arg1	vertebrate					1910:1919	vertebrate	1910:1919	vertebrate	1910:1919	This work allows us to better understand the relationship among gut microbiota, nutrition metabolism and feeding habits in vertebrate.					
30176798	10	30	theme	PICRUSt	1451:1457	arg1	test					1459:1462	PICRUSt test	1451:1462	PICRUSt test	1451:1462	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	10	31	theme	canonical	1271:1279	arg1	RDA					1302:1304	RDA	1302:1304	RDA	1302:1304	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	10	31	theme	canonical	1271:1279	arg1	analysis					1292:1299	The canonical redundancy analysis	1267:1299	The canonical redundancy analysis (RDA)	1267:1305	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	5	32	theme	Taxonomic	708:716	arg1	Units					718:722	388 Operational Taxonomic Units	692:722	388 Operational Taxonomic Units	692:722	The valid sequences were classified into 388 Operational Taxonomic Units, and a total of 223 genera, belonging to 20 phyla, were identified.					
30176798	4	33	dep	RESULTS	517:523	arg1	obtained					601:608	obtained	601:608	were obtained using the Illumina MiSeq PE300 platform	596:648	RESULTS In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform.					
30176798	2	34	theme	vital	355:359	arg1	role					361:364	a vital role	353:364	a vital role	353:364	Gut microbiota has a vital role to host animal.					
30176798	12	35	theme	nutrition	1867:1875	arg1	metabolism					1877:1886	nutrition metabolism	1867:1886	nutrition metabolism	1867:1886	This work allows us to better understand the relationship among gut microbiota, nutrition metabolism and feeding habits in vertebrate.					
30176798	0	36	theme	intestinal	25:34	arg1	microbiota					36:45	intestinal microbiota	25:45	intestinal microbiota in blunt snout bream (Megalobrama amblycephala)	25:93	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	10	37	theme	gut	1504:1506	arg1	microbiota					1508:1517	gut microbiota	1504:1517	gut microbiota	1504:1517	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	11	38	theme	transition	1667:1676	arg1	period					1678:1683	its dietary transition period	1655:1683	its dietary transition period	1655:1683	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	5	39	theme	genera	744:749	arg1	total					731:735	a total	729:735	a total	729:735	The valid sequences were classified into 388 Operational Taxonomic Units, and a total of 223 genera, belonging to 20 phyla, were identified.					
30176798	3	40	theme	feeding	437:443	arg1	transition					452:461	transition	452:461	transition	452:461	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	3	40	theme	feeding	437:443	arg1	microbiota					468:477	gut microbiota	464:477	gut microbiota	464:477	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	3	40	theme	feeding	437:443	arg1	habits					445:450	feeding habits transition, gut microbiota and digestive enzymes of gut content	437:514	feeding habits transition, gut microbiota and digestive enzymes of gut content	437:514	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	11	41	theme	first	1693:1697	arg1	time					1699:1702	the first time	1689:1702	the first time	1689:1702	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	11	42	from	survey	1598:1603	arg1	bream					1642:1646	blunt snout bream	1630:1646	blunt snout bream	1630:1646	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	1	43	theme	commercial	217:226	arg1	fish					240:243	the most important commercial herbivorous fish	198:243	the most important commercial herbivorous fish in China	198:252	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	8	44	theme	gut	1091:1093	arg1	microbiota					1095:1104	gut microbiota	1091:1104	gut microbiota	1091:1104	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	11	45	dep	bream	1642:1646	arg1	blunt					1630:1634	blunt	1630:1634	blunt	1630:1634	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	1	46	from	event	294:298	arg1	development					321:331	blunt snout bream development	303:331	blunt snout bream development	303:331	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	3	47	theme	gut	464:466	arg1	microbiota					468:477	gut microbiota	464:477	gut microbiota	464:477	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	3	47	theme	gut	464:466	arg1	habits					445:450	feeding habits transition, gut microbiota and digestive enzymes of gut content	437:514	feeding habits transition, gut microbiota and digestive enzymes of gut content	437:514	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	1	48	theme	fish	240:243	arg1	one					191:193	one	191:193	one	191:193	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	48	theme	fish	240:243	arg1	fish					240:243	the most important commercial herbivorous fish	198:243	the most important commercial herbivorous fish in China	198:252	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	48	theme	fish	240:243	arg1	bream					155:159	The blunt snout bream	139:159	The blunt snout bream (Megalobrama amblycephala)	139:186	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	11	49	theme	microbiota	1616:1625	arg1	survey					1598:1603	a comprehensive survey	1582:1603	a comprehensive survey of the gut microbiota in blunt snout bream	1582:1646	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	3	50	theme	gut	504:506	arg1	content					508:514	gut content	504:514	gut content	504:514	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	8	51	theme	diet	1127:1130	arg1	stages					1132:1137	zooplankton-based diet stages	1109:1137	zooplankton-based diet stages	1109:1137	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	6	52	theme	communities	831:841	arg1	related					859:865	related	859:865	related	859:865	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	6	52	theme	communities	831:841	arg1	result					807:812	The clustering result	792:812	The clustering result of gut bacterial communities	792:841	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	10	53	theme	gut	1364:1366	arg1	microbiota					1368:1377	the gut microbiota	1360:1377	the gut microbiota	1360:1377	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	6	54	theme	gut	817:819	arg1	communities					831:841	gut bacterial communities	817:841	gut bacterial communities	817:841	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	8	55	theme	transition	1148:1157	arg1	stages					1159:1164	diet transition stages	1143:1164	diet transition stages	1143:1164	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	4	56	theme	Illumina	620:627	arg1	platform					641:648	the Illumina MiSeq PE300 platform	616:648	the Illumina MiSeq PE300 platform	616:648	RESULTS In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform.					
30176798	5	57	theme	valid	655:659	arg1	sequences					661:669	The valid sequences	651:669	The valid sequences	651:669	The valid sequences were classified into 388 Operational Taxonomic Units, and a total of 223 genera, belonging to 20 phyla, were identified.					
30176798	10	58	theme	digestive	1387:1395	arg1	activities					1404:1413	the digestive enzyme activities	1383:1413	the digestive enzyme activities of gut content	1383:1428	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	6	59	theme	content	906:912	arg1	compositions					914:925	intestinal content compositions	895:925	intestinal content compositions	895:925	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	4	60	theme	rRNA	581:584	arg1	libraries					586:594	nine 16S rRNA libraries	572:594	nine 16S rRNA libraries	572:594	RESULTS In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform.					
30176798	7	61	theme	snout	1004:1008	arg1	bream					1010:1014	blunt snout bream	998:1014	blunt snout bream	998:1014	Proteobacteria and Firmicutes constitute the 'core' gut microbiota of blunt snout bream.					
30176798	9	62	theme	cellulose-degrading	1210:1228	arg1	bacteria					1230:1237	thirteen potential cellulose-degrading bacteria	1191:1237	thirteen potential cellulose-degrading bacteria	1191:1237	Moreover, thirteen potential cellulose-degrading bacteria were detected in our study.					
30176798	0	63	from	bream	62:66	arg1	profile					14:20	The variation profile	0:20	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.	0:126	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	6	64	theme	clustering	874:883	arg1	result					885:890	the clustering result	870:890	the clustering result of intestinal content compositions	870:925	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	7	65	theme	gut	980:982	arg1	microbiota					984:993	the 'core' gut microbiota	969:993	the 'core' gut microbiota of blunt snout bream	969:1014	Proteobacteria and Firmicutes constitute the 'core' gut microbiota of blunt snout bream.					
30176798	13	66	theme	life	2081:2084	arg1	history					2086:2092	their life history	2075:2092	their life history	2075:2092	Further, our study provides a reference for future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history.					
30176798	4	67	theme	PE300	635:639	arg1	platform					641:648	the Illumina MiSeq PE300 platform	616:648	the Illumina MiSeq PE300 platform	616:648	RESULTS In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform.					
30176798	1	68	theme	snout	309:313	arg1	development					321:331	blunt snout bream development	303:331	blunt snout bream development	303:331	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	12	69	theme	feeding	1892:1898	arg1	habits					1900:1905	feeding habits	1892:1905	feeding habits in vertebrate	1892:1919	This work allows us to better understand the relationship among gut microbiota, nutrition metabolism and feeding habits in vertebrate.					
30176798	1	70	theme	snout	149:153	arg1	amblycephala					174:185	Megalobrama amblycephala	162:185	Megalobrama amblycephala	162:185	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	70	theme	snout	149:153	arg1	one					191:193	one	191:193	one	191:193	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	70	theme	snout	149:153	arg1	fish					240:243	the most important commercial herbivorous fish	198:243	the most important commercial herbivorous fish in China	198:252	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	70	theme	snout	149:153	arg1	bream					155:159	The blunt snout bream	139:159	The blunt snout bream (Megalobrama amblycephala)	139:186	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	12	71	from	metabolism	1877:1886	arg1	vertebrate					1910:1919	vertebrate	1910:1919	vertebrate	1910:1919	This work allows us to better understand the relationship among gut microbiota, nutrition metabolism and feeding habits in vertebrate.					
30176798	4	72	dep	high-quality	548:559	arg1	reads					561:565	reads	561:565	reads	561:565	RESULTS In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform.					
30176798	13	73	theme	vegetarian	2052:2061	arg1	diet					2063:2066	a vegetarian diet	2050:2066	a vegetarian diet during their life history	2050:2092	Further, our study provides a reference for future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history.					
30176798	10	74	theme	gut	1418:1420	arg1	content					1422:1428	gut content	1418:1428	gut content	1418:1428	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	1	75	dep	BACKGROUND	128:137	arg1	transition					267:276	dietary transition	259:276	dietary transition	259:276	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	75	dep	BACKGROUND	128:137	arg1	one					191:193	one	191:193	one	191:193	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	75	dep	BACKGROUND	128:137	arg1	event					294:298	an important event	281:298	an important event in blunt snout bream development	281:331	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	75	dep	BACKGROUND	128:137	arg1	fish					240:243	the most important commercial herbivorous fish	198:243	the most important commercial herbivorous fish in China	198:252	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	75	dep	BACKGROUND	128:137	arg1	bream					155:159	The blunt snout bream	139:159	The blunt snout bream (Megalobrama amblycephala)	139:186	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	10	76	theme	redundancy	1281:1290	arg1	RDA					1302:1304	RDA	1302:1304	RDA	1302:1304	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	10	76	theme	redundancy	1281:1290	arg1	analysis					1292:1299	The canonical redundancy analysis	1267:1299	The canonical redundancy analysis (RDA)	1267:1305	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	0	77	theme	feeding	102:108	arg1	transition					116:125	feeding habit transition	102:125	feeding habit transition	102:125	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	13	78	theme	herbivorous	2021:2031	arg1	fish					2033:2036	herbivorous fish	2021:2036	herbivorous fish	2021:2036	Further, our study provides a reference for future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history.					
30176798	0	79	from	microbiota	36:45	arg1	bream					62:66	blunt snout bream	50:66	blunt snout bream (Megalobrama amblycephala)	50:93	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	0	79	from	microbiota	36:45	arg1	amblycephala					81:92	amblycephala	81:92	amblycephala	81:92	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	5	80	theme	Operational	696:706	arg1	Units					718:722	388 Operational Taxonomic Units	692:722	388 Operational Taxonomic Units	692:722	The valid sequences were classified into 388 Operational Taxonomic Units, and a total of 223 genera, belonging to 20 phyla, were identified.					
30176798	0	81	theme	variation	4:12	arg1	profile					14:20	The variation profile	0:20	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.	0:126	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	2	82	contain	has	349:351	arg1	microbiota					338:347	Gut microbiota	334:347	Gut microbiota	334:347	Gut microbiota has a vital role to host animal.					
30176798	2	82	contain	has	349:351	arg2	role					361:364	a vital role	353:364	a vital role	353:364	Gut microbiota has a vital role to host animal.					
30176798	1	83	from	fish	240:243	arg1	China					248:252	China	248:252	China	248:252	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	84	theme	dietary	259:265	arg1	transition					267:276	dietary transition	259:276	dietary transition	259:276	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	84	theme	dietary	259:265	arg1	event					294:298	an important event	281:298	an important event in blunt snout bream development	281:331	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	0	85	dep	bream	62:66	arg1	blunt					50:54	blunt	50:54	blunt	50:54	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	0	86	theme	microbiota	36:45	arg1	profile					14:20	The variation profile	0:20	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.	0:126	The variation profile of intestinal microbiota in blunt snout bream (Megalobrama amblycephala) during feeding habit transition.					
30176798	12	87	theme	gut	1851:1853	arg1	microbiota					1855:1864	gut microbiota	1851:1864	gut microbiota	1851:1864	This work allows us to better understand the relationship among gut microbiota, nutrition metabolism and feeding habits in vertebrate.					
30176798	6	88	theme	clustering	796:805	arg1	related					859:865	related	859:865	related	859:865	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	6	88	theme	clustering	796:805	arg1	result					807:812	The clustering result	792:812	The clustering result of gut bacterial communities	792:841	The clustering result of gut bacterial communities is consistently related to the clustering result of intestinal content compositions.					
30176798	10	89	theme	test	1459:1462	arg1	result					1441:1446	the result	1437:1446	the result of PICRUSt test	1437:1462	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	11	90	theme	dietary	1659:1665	arg1	period					1678:1683	its dietary transition period	1655:1683	its dietary transition period	1655:1683	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	10	91	theme	feeding	1325:1331	arg1	habits					1333:1338	the feeding habits	1321:1338	the feeding habits	1321:1338	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	2	92	theme	host	369:372	arg1	animal					374:379	host animal	369:379	host animal	369:379	Gut microbiota has a vital role to host animal.					
30176798	1	93	theme	important	284:292	arg1	transition					267:276	dietary transition	259:276	dietary transition	259:276	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	1	93	theme	important	284:292	arg1	event					294:298	an important event	281:298	an important event in blunt snout bream development	281:331	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	8	94	theme	microbiological	1064:1078	arg1	markers					1080:1086	microbiological markers	1064:1086	microbiological markers of gut microbiota	1064:1104	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	8	94	theme	microbiological	1064:1078	arg1	Rhizobium					1035:1043	Rhizobium	1035:1043	Rhizobium	1035:1043	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	8	94	theme	microbiological	1064:1078	arg1	Cetobacterium					1017:1029	Cetobacterium	1017:1029	Cetobacterium	1017:1029	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	13	95	theme	fish	2033:2036	arg1	adaption					2009:2016	the metabolic adaption	1995:2016	the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history	1995:2092	Further, our study provides a reference for future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history.					
30176798	13	96	theme	metabolic	1999:2007	arg1	adaption					2009:2016	the metabolic adaption	1995:2016	the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history	1995:2092	Further, our study provides a reference for future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history.					
30176798	13	97	theme	future	1966:1971	arg1	studies					1973:1979	future studies	1966:1979	future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history	1966:2092	Further, our study provides a reference for future studies investigating the metabolic adaption of herbivorous fish to shift to a vegetarian diet during their life history.					
30176798	10	98	theme	metabolic	1482:1490	arg1	capacity					1492:1499	the metabolic capacity	1478:1499	the metabolic capacity of gut microbiota	1478:1517	The canonical redundancy analysis (RDA) revealed that the feeding habits strongly influenced the gut microbiota and the digestive enzyme activities of gut content, while the result of PICRUSt test suggests that the metabolic capacity of gut microbiota was affected by feeding habit.					
30176798	1	99	theme	important	207:215	arg1	fish					240:243	the most important commercial herbivorous fish	198:243	the most important commercial herbivorous fish in China	198:252	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30176798	8	100	theme	microbiota	1095:1104	arg1	markers					1080:1086	microbiological markers	1064:1086	microbiological markers of gut microbiota	1064:1104	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	8	100	theme	microbiota	1095:1104	arg1	Rhizobium					1035:1043	Rhizobium	1035:1043	Rhizobium	1035:1043	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	8	100	theme	microbiota	1095:1104	arg1	Cetobacterium					1017:1029	Cetobacterium	1017:1029	Cetobacterium	1017:1029	Cetobacterium and Rhizobium were identified as microbiological markers of gut microbiota at zooplankton-based diet stages and diet transition stages, respectively.					
30176798	4	101	theme	16S	577:579	arg1	libraries					586:594	nine 16S rRNA libraries	572:594	nine 16S rRNA libraries	572:594	RESULTS In this study, 186,328 high-quality reads from nine 16S rRNA libraries were obtained using the Illumina MiSeq PE300 platform.					
30176798	7	102	dep	bream	1010:1014	arg1	blunt					998:1002	blunt	998:1002	blunt	998:1002	Proteobacteria and Firmicutes constitute the 'core' gut microbiota of blunt snout bream.					
30176798	11	103	theme	comprehensive	1584:1596	arg1	survey					1598:1603	a comprehensive survey	1582:1603	a comprehensive survey of the gut microbiota in blunt snout bream	1582:1646	CONCLUSIONS This study provided a comprehensive survey of the gut microbiota in blunt snout bream during its dietary transition period for the first time and clearly showed that the gut microbiota was strongly affected by feeding habit.					
30176798	3	104	theme	digestive	483:491	arg1	enzymes					493:499	digestive enzymes	483:499	digestive enzymes of gut content	483:514	However, little was known about the relationship among feeding habits transition, gut microbiota and digestive enzymes of gut content.					
30176798	1	105	theme	herbivorous	228:238	arg1	fish					240:243	the most important commercial herbivorous fish	198:243	the most important commercial herbivorous fish in China	198:252	BACKGROUND The blunt snout bream (Megalobrama amblycephala) is one of the most important commercial herbivorous fish in China, and dietary transition is an important event in blunt snout bream development.					
30851581	8	0	theme	-induced	1065:1072	arg1	model					1088:1092	Dextran sulfate sodium (DSS)-induced acute colitis model	1037:1092	Dextran sulfate sodium (DSS)-induced acute colitis model	1037:1092	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	4	1	theme	PURPOSE	528:534	arg1	TG-n2					564:568	TG-n2	564:568	TG-n2	564:568	PURPOSE Tripterygium glycosides-n2 (TG-n2) was obtained from TG by a new preparation method.					
30851581	4	1	theme	PURPOSE	528:534	arg1	glycosides-n2					549:561	PURPOSE Tripterygium glycosides-n2	528:561	PURPOSE Tripterygium glycosides-n2 (TG-n2)	528:569	PURPOSE Tripterygium glycosides-n2 (TG-n2) was obtained from TG by a new preparation method.					
30851581	3	2	theme	new	458:460	arg1	method					462:467	a new method	456:467	a new method to obtain the attenuated and synergistic extracts from TG	456:525	Therefore, it is necessary to study a new method to obtain the attenuated and synergistic extracts from TG.					
30851581	8	3	theme	colitis	1080:1086	arg1	model					1088:1092	Dextran sulfate sodium (DSS)-induced acute colitis model	1037:1092	Dextran sulfate sodium (DSS)-induced acute colitis model	1037:1092	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	6	4	theme	chemical	830:837	arg1	compositions					839:850	The major chemical compositions	820:850	METHODS The major chemical compositions of TG and TG-n2	812:866	METHODS The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC).					
30851581	12	5	theme	body	1582:1585	arg1	loss					1594:1597	body weight loss	1582:1597	body weight loss	1582:1597	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	5	theme	body	1582:1585	arg1	symptoms					1528:1535	the symptoms	1524:1535	the symptoms	1524:1535	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	5	theme	body	1582:1585	arg1	diarrhea					1557:1564	diarrhea	1557:1564	diarrhea	1557:1564	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	17	6	from	inflammation	2295:2306	arg1	colon					2311:2315	colon	2311:2315	colon	2311:2315	Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.					
30851581	16	7	theme	attenuated	2001:2010	arg1	TG-n2					2012:2016	the attenuated TG-n2	1997:2016	the attenuated TG-n2	1997:2016	Moreover, the attenuated TG-n2 has the similar therapeutic effect on treating experimental colitis in mice when compared to TG.					
30851581	15	8	theme	TG-n2	1963:1967	arg1	administration					1945:1958	oral administration	1940:1958	oral administration of TG-n2	1940:1967	CONCLUSION This study showed that oral administration of TG-n2 is safer than TG.					
30851581	4	9	theme	new	597:599	arg1	method					613:618	a new preparation method	595:618	a new preparation method	595:618	PURPOSE Tripterygium glycosides-n2 (TG-n2) was obtained from TG by a new preparation method.					
30851581	12	10	dep	has	1458:1460	arg1	reducing					1679:1686	reducing	1679:1686	reducing absolute lymph number	1679:1708	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	10	dep	has	1458:1460	arg1	inhibiting					1644:1653	inhibiting	1644:1653	inhibiting cytokines secretion	1644:1673	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	1	11	theme	main	164:167	arg1	BACKGROUND					113:122	BACKGROUND	113:122	BACKGROUND The Tripterygium glycosides (TG)	113:155	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	1	11	theme	main	164:167	arg1	extractive					176:185	the main active extractive	160:185	the main active extractive of Tripterygium wilfordii Hook F	160:218	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	13	12	theme	T	1767:1767	arg1	lymphocyte					1769:1778	T lymphocyte	1767:1778	T lymphocyte	1767:1778	In addition, TG and TG-n2 can increase the apoptosis of T lymphocyte in vivo.					
30851581	6	13	theme	liquid	903:908	arg1	UPLC					926:929	UPLC	926:929	UPLC	926:929	METHODS The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC).					
30851581	6	13	theme	liquid	903:908	arg1	chromatography					910:923	ultra-performance liquid chromatography	885:923	ultra-performance liquid chromatography (UPLC)	885:930	METHODS The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC).					
30851581	5	14	from	difference	664:673	arg1	compositions					691:702	the chemical compositions	678:702	the chemical compositions	678:702	In this study, we aimed to investigate the difference in the chemical compositions between TG and TG-n2, further explored its toxicity and therapeutic effects on DSS-induced colitis in mice.					
30851581	5	15	theme	therapeutic	760:770	arg1	effects					772:778	its toxicity and therapeutic effects	743:778	its toxicity and therapeutic effects on DSS-induced colitis in mice	743:809	In this study, we aimed to investigate the difference in the chemical compositions between TG and TG-n2, further explored its toxicity and therapeutic effects on DSS-induced colitis in mice.					
30851581	8	16	used	used	1098:1101	arg2	model					1088:1092	Dextran sulfate sodium (DSS)-induced acute colitis model	1037:1092	Dextran sulfate sodium (DSS)-induced acute colitis model	1037:1092	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	17	17	theme	lymphocytes	2252:2262	arg1	survival					2238:2245	the survival	2234:2245	the survival of T lymphocytes in colitis mice	2234:2278	Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.					
30851581	8	18	theme	action	1187:1192	arg1	effect					1130:1135	the therapeutic effect	1114:1135	the therapeutic effect of TG and TG-n2	1114:1151	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	8	18	theme	action	1187:1192	arg1	mechanisms					1173:1182	their potential mechanisms	1157:1182	their potential mechanisms of action	1157:1192	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	7	19	theme	acute	947:951	arg1	test					962:965	acute toxicity test	947:965	acute toxicity test	947:965	Subsequently, acute toxicity test was applied to evaluate the toxicity difference between TG and TG-n2.					
30851581	1	20	theme	Hook	213:216	arg1	F					218:218	Tripterygium wilfordii Hook F	190:218	Tripterygium wilfordii Hook F	190:218	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	8	21	theme	TG	1140:1141	arg1	effect					1130:1135	the therapeutic effect	1114:1135	the therapeutic effect of TG and TG-n2	1114:1151	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	8	21	theme	TG	1140:1141	arg1	mechanisms					1173:1182	their potential mechanisms	1157:1182	their potential mechanisms of action	1157:1192	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	12	22	theme	further	1424:1430	arg1	studies					1432:1438	further studies	1424:1438	further studies	1424:1438	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	17	23	from	survival	2238:2245	arg1	mice					2275:2278	colitis mice	2267:2278	colitis mice	2267:2278	Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.					
30851581	17	24	from	expression	2162:2171	arg1	cells					2185:2189	T cells	2183:2189	T cells	2183:2189	Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.					
30851581	5	25	dep	aimed	639:643	arg1	explored					734:741	explored	734:741	further explored its toxicity and therapeutic effects on DSS-induced colitis in mice	726:809	In this study, we aimed to investigate the difference in the chemical compositions between TG and TG-n2, further explored its toxicity and therapeutic effects on DSS-induced colitis in mice.					
30851581	11	26	theme	toxicity	1354:1361	arg1	test					1363:1366	Acute toxicity test	1348:1366	Acute toxicity test	1348:1366	Acute toxicity test proved that TG-n2 was less toxic than TG.					
30851581	5	27	theme	chemical	682:689	arg1	compositions					691:702	the chemical compositions	678:702	the chemical compositions	678:702	In this study, we aimed to investigate the difference in the chemical compositions between TG and TG-n2, further explored its toxicity and therapeutic effects on DSS-induced colitis in mice.					
30851581	5	28	theme	toxicity	747:754	arg1	effects					772:778	its toxicity and therapeutic effects	743:778	its toxicity and therapeutic effects on DSS-induced colitis in mice	743:809	In this study, we aimed to investigate the difference in the chemical compositions between TG and TG-n2, further explored its toxicity and therapeutic effects on DSS-induced colitis in mice.					
30851581	12	29	theme	colitis	1540:1546	arg1	diarrhea					1557:1564	diarrhea	1557:1564	diarrhea	1557:1564	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	29	theme	colitis	1540:1546	arg1	loss					1594:1597	body weight loss	1582:1597	body weight loss	1582:1597	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	29	theme	colitis	1540:1546	arg1	symptoms					1528:1535	the symptoms	1524:1535	the symptoms	1524:1535	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	29	theme	colitis	1540:1546	arg1	changes					1635:1641	histopathological changes	1617:1641	histopathological changes	1617:1641	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	29	theme	colitis	1540:1546	arg1	stools					1574:1579	bloody stools	1567:1579	bloody stools	1567:1579	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	29	theme	colitis	1540:1546	arg1	atrophy					1608:1614	colonic atrophy	1600:1614	colonic atrophy	1600:1614	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	9	30	from	TG	1270:1271	arg1	different					1255:1263	different	1255:1263	different	1255:1263	RESULTS We found that the chemical compositions of TG-n2 is different from TG.					
30851581	9	30	from	TG	1270:1271	arg1	compositions					1230:1241	the chemical compositions	1217:1241	the chemical compositions of TG-n2	1217:1250	RESULTS We found that the chemical compositions of TG-n2 is different from TG.					
30851581	1	31	dep	BACKGROUND	113:122	arg1	TG					153:154	TG	153:154	TG	153:154	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	1	31	dep	BACKGROUND	113:122	arg1	glycosides					141:150	The Tripterygium glycosides	124:150	BACKGROUND The Tripterygium glycosides (TG)	113:155	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	16	32	theme	similar	2026:2032	arg1	effect					2046:2051	the similar therapeutic effect	2022:2051	the similar therapeutic effect	2022:2051	Moreover, the attenuated TG-n2 has the similar therapeutic effect on treating experimental colitis in mice when compared to TG.					
30851581	9	33	theme	chemical	1221:1228	arg1	different					1255:1263	different	1255:1263	different	1255:1263	RESULTS We found that the chemical compositions of TG-n2 is different from TG.					
30851581	9	33	theme	chemical	1221:1228	arg1	compositions					1230:1241	the chemical compositions	1217:1241	the chemical compositions of TG-n2	1217:1250	RESULTS We found that the chemical compositions of TG-n2 is different from TG.					
30851581	2	34	theme	diseases	410:417	arg1	treatment					392:400	the treatment	388:400	the treatment of many diseases	388:417	However, due to its severe toxicity, TG is restricted to the treatment of many diseases.					
30851581	0	35	theme	Tripterygium	0:11	arg1	n2					32:33	Tripterygium glycoside fraction n2	0:33	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.	0:111	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.					
30851581	14	36	theme	CD3-positive	1884:1895	arg1	cells					1899:1903	CD3-positive T cells	1884:1903	CD3-positive T cells	1884:1903	Further investigated showed that TG and TG-n2 could increase the expressions of Bax and p62 on CD3-positive T cells.					
30851581	0	37	theme	fraction	23:30	arg1	n2					32:33	Tripterygium glycoside fraction n2	0:33	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.	0:111	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.					
30851581	1	38	theme	inflammatory	269:280	arg1	disease					321:327	inflammatory bowel disease	302:327	inflammatory bowel disease	302:327	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	1	38	theme	inflammatory	269:280	arg1	diseases					282:289	inflammatory diseases	269:289	inflammatory diseases (including inflammatory bowel disease)	269:328	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	12	39	theme	lymph	1697:1701	arg1	number					1703:1708	absolute lymph number	1688:1708	absolute lymph number	1688:1708	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	9	40	dep	RESULTS	1195:1201	arg1	found					1206:1210	found	1206:1210	found that the chemical compositions of TG-n2 is different from TG	1206:1271	RESULTS We found that the chemical compositions of TG-n2 is different from TG.					
30851581	14	41	theme	p62	1877:1879	arg1	expressions					1854:1864	the expressions	1850:1864	the expressions of Bax and p62 on CD3-positive T cells	1850:1903	Further investigated showed that TG and TG-n2 could increase the expressions of Bax and p62 on CD3-positive T cells.					
30851581	3	42	from	TG	524:525	arg1	extracts					510:517	the attenuated and synergistic extracts	479:517	the attenuated and synergistic extracts from TG	479:525	Therefore, it is necessary to study a new method to obtain the attenuated and synergistic extracts from TG.					
30851581	8	43	theme	TG-n2	1147:1151	arg1	effect					1130:1135	the therapeutic effect	1114:1135	the therapeutic effect of TG and TG-n2	1114:1151	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	8	43	theme	TG-n2	1147:1151	arg1	mechanisms					1173:1182	their potential mechanisms	1157:1182	their potential mechanisms of action	1157:1192	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	16	44	contain	has	2018:2020	arg1	TG-n2					2012:2016	the attenuated TG-n2	1997:2016	the attenuated TG-n2	1997:2016	Moreover, the attenuated TG-n2 has the similar therapeutic effect on treating experimental colitis in mice when compared to TG.					
30851581	16	44	contain	has	2018:2020	arg2	effect					2046:2051	the similar therapeutic effect	2022:2051	the similar therapeutic effect	2022:2051	Moreover, the attenuated TG-n2 has the similar therapeutic effect on treating experimental colitis in mice when compared to TG.					
30851581	15	45	dep	CONCLUSION	1906:1915	arg1	showed					1928:1933	showed	1928:1933	showed that oral administration of TG-n2 is safer than TG	1928:1984	CONCLUSION This study showed that oral administration of TG-n2 is safer than TG.					
30851581	14	46	theme	Bax	1869:1871	arg1	expressions					1854:1864	the expressions	1850:1864	the expressions of Bax and p62 on CD3-positive T cells	1850:1903	Further investigated showed that TG and TG-n2 could increase the expressions of Bax and p62 on CD3-positive T cells.					
30851581	0	47	theme	colitis	63:69	arg1	Alleviation					36:46	Alleviation	36:46	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.	0:111	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.					
30851581	1	48	theme	inflammatory	302:313	arg1	disease					321:327	inflammatory bowel disease	302:327	inflammatory bowel disease	302:327	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	14	49	from	expressions	1854:1864	arg1	cells					1899:1903	CD3-positive T cells	1884:1903	CD3-positive T cells	1884:1903	Further investigated showed that TG and TG-n2 could increase the expressions of Bax and p62 on CD3-positive T cells.					
30851581	12	50	theme	cytokines	1655:1663	arg1	secretion					1665:1673	cytokines secretion	1655:1673	cytokines secretion	1655:1673	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	10	51	dep	ratio	1301:1305	arg1	T9					1343:1344	T9	1343:1344	T9	1343:1344	The main difference is the ratio of triptriolide (T11) / triptolide (T9).					
30851581	10	51	dep	ratio	1301:1305	arg1	triptolide					1331:1340	triptolide	1331:1340	the ratio of triptriolide (T11) / triptolide (T9)	1297:1345	The main difference is the ratio of triptriolide (T11) / triptolide (T9).					
30851581	1	52	theme	F	218:218	arg1	BACKGROUND					113:122	BACKGROUND	113:122	BACKGROUND The Tripterygium glycosides (TG)	113:155	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	1	52	theme	F	218:218	arg1	extractive					176:185	the main active extractive	160:185	the main active extractive of Tripterygium wilfordii Hook F	160:218	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	16	53	from	colitis	2078:2084	arg1	mice					2089:2092	mice	2089:2092	mice when compared to TG	2089:2112	Moreover, the attenuated TG-n2 has the similar therapeutic effect on treating experimental colitis in mice when compared to TG.					
30851581	6	54	theme	TG	855:856	arg1	compositions					839:850	The major chemical compositions	820:850	METHODS The major chemical compositions of TG and TG-n2	812:866	METHODS The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC).					
30851581	12	55	theme	histopathological	1617:1633	arg1	symptoms					1528:1535	the symptoms	1524:1535	the symptoms	1524:1535	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	55	theme	histopathological	1617:1633	arg1	changes					1635:1641	histopathological changes	1617:1641	histopathological changes	1617:1641	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	55	theme	histopathological	1617:1633	arg1	diarrhea					1557:1564	diarrhea	1557:1564	diarrhea	1557:1564	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	56	contain	has	1458:1460	arg2	effect					1484:1489	a similar therapeutic effect	1462:1489	a similar therapeutic effect	1462:1489	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	56	contain	has	1458:1460	arg1	TG-n2					1452:1456	TG-n2	1452:1456	TG-n2	1452:1456	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	57	theme	weight	1587:1592	arg1	loss					1594:1597	body weight loss	1582:1597	body weight loss	1582:1597	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	57	theme	weight	1587:1592	arg1	symptoms					1528:1535	the symptoms	1524:1535	the symptoms	1524:1535	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	57	theme	weight	1587:1592	arg1	diarrhea					1557:1564	diarrhea	1557:1564	diarrhea	1557:1564	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	3	58	theme	attenuated	483:492	arg1	extracts					510:517	the attenuated and synergistic extracts	479:517	the attenuated and synergistic extracts from TG	479:525	Therefore, it is necessary to study a new method to obtain the attenuated and synergistic extracts from TG.					
30851581	1	59	theme	clinical	242:249	arg1	practice					251:258	clinical practice	242:258	clinical practice	242:258	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	8	60	theme	acute	1074:1078	arg1	model					1088:1092	Dextran sulfate sodium (DSS)-induced acute colitis model	1037:1092	Dextran sulfate sodium (DSS)-induced acute colitis model	1037:1092	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	6	61	theme	major	824:828	arg1	compositions					839:850	The major chemical compositions	820:850	METHODS The major chemical compositions of TG and TG-n2	812:866	METHODS The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC).					
30851581	4	62	theme	Tripterygium	536:547	arg1	TG-n2					564:568	TG-n2	564:568	TG-n2	564:568	PURPOSE Tripterygium glycosides-n2 (TG-n2) was obtained from TG by a new preparation method.					
30851581	4	62	theme	Tripterygium	536:547	arg1	glycosides-n2					549:561	PURPOSE Tripterygium glycosides-n2	528:561	PURPOSE Tripterygium glycosides-n2 (TG-n2)	528:569	PURPOSE Tripterygium glycosides-n2 (TG-n2) was obtained from TG by a new preparation method.					
30851581	10	63	theme	main	1278:1281	arg1	difference					1283:1292	The main difference	1274:1292	The main difference	1274:1292	The main difference is the ratio of triptriolide (T11) / triptolide (T9).					
30851581	10	63	theme	main	1278:1281	arg1	ratio					1301:1305	the ratio	1297:1305	the ratio of triptriolide (T11) / triptolide (T9)	1297:1345	The main difference is the ratio of triptriolide (T11) / triptolide (T9).					
30851581	5	64	theme	DSS-induced	783:793	arg1	colitis					795:801	DSS-induced colitis	783:801	DSS-induced colitis in mice	783:809	In this study, we aimed to investigate the difference in the chemical compositions between TG and TG-n2, further explored its toxicity and therapeutic effects on DSS-induced colitis in mice.					
30851581	12	65	theme	bloody	1567:1572	arg1	stools					1574:1579	bloody stools	1567:1579	bloody stools	1567:1579	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	65	theme	bloody	1567:1572	arg1	symptoms					1528:1535	the symptoms	1524:1535	the symptoms	1524:1535	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	65	theme	bloody	1567:1572	arg1	diarrhea					1557:1564	diarrhea	1557:1564	diarrhea	1557:1564	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	10	66	theme	triptriolide	1310:1321	arg1	T11					1324:1326	T11	1324:1326	T11	1324:1326	The main difference is the ratio of triptriolide (T11) / triptolide (T9).					
30851581	10	66	theme	triptriolide	1310:1321	arg1	difference					1283:1292	The main difference	1274:1292	The main difference	1274:1292	The main difference is the ratio of triptriolide (T11) / triptolide (T9).					
30851581	10	66	theme	triptriolide	1310:1321	arg1	ratio					1301:1305	the ratio	1297:1305	the ratio of triptriolide (T11) / triptolide (T9)	1297:1345	The main difference is the ratio of triptriolide (T11) / triptolide (T9).					
30851581	1	67	theme	active	169:174	arg1	BACKGROUND					113:122	BACKGROUND	113:122	BACKGROUND The Tripterygium glycosides (TG)	113:155	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	1	67	theme	active	169:174	arg1	extractive					176:185	the main active extractive	160:185	the main active extractive of Tripterygium wilfordii Hook F	160:218	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	4	68	theme	preparation	601:611	arg1	method					613:618	a new preparation method	595:618	a new preparation method	595:618	PURPOSE Tripterygium glycosides-n2 (TG-n2) was obtained from TG by a new preparation method.					
30851581	7	69	theme	toxicity	995:1002	arg1	difference					1004:1013	the toxicity difference	991:1013	the toxicity difference between TG and TG-n2	991:1034	Subsequently, acute toxicity test was applied to evaluate the toxicity difference between TG and TG-n2.					
30851581	13	70	theme	lymphocyte	1769:1778	arg1	apoptosis					1754:1762	the apoptosis	1750:1762	the apoptosis of T lymphocyte	1750:1778	In addition, TG and TG-n2 can increase the apoptosis of T lymphocyte in vivo.					
30851581	3	71	theme	synergistic	498:508	arg1	extracts					510:517	the attenuated and synergistic extracts	479:517	the attenuated and synergistic extracts from TG	479:525	Therefore, it is necessary to study a new method to obtain the attenuated and synergistic extracts from TG.					
30851581	6	72	theme	ultra-performance	885:901	arg1	UPLC					926:929	UPLC	926:929	UPLC	926:929	METHODS The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC).					
30851581	6	72	theme	ultra-performance	885:901	arg1	chromatography					910:923	ultra-performance liquid chromatography	885:923	ultra-performance liquid chromatography (UPLC)	885:930	METHODS The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC).					
30851581	17	73	theme	T	2250:2250	arg1	lymphocytes					2252:2262	T lymphocytes	2250:2262	T lymphocytes	2250:2262	Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.					
30851581	8	74	theme	potential	1163:1171	arg1	mechanisms					1173:1182	their potential mechanisms	1157:1182	their potential mechanisms of action	1157:1192	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	15	75	theme	oral	1940:1943	arg1	administration					1945:1958	oral administration	1940:1958	oral administration of TG-n2	1940:1967	CONCLUSION This study showed that oral administration of TG-n2 is safer than TG.					
30851581	8	76	theme	therapeutic	1118:1128	arg1	effect					1130:1135	the therapeutic effect	1114:1135	the therapeutic effect of TG and TG-n2	1114:1151	Dextran sulfate sodium (DSS)-induced acute colitis model was used to explore the therapeutic effect of TG and TG-n2 and their potential mechanisms of action.					
30851581	7	77	theme	toxicity	953:960	arg1	test					962:965	acute toxicity test	947:965	acute toxicity test	947:965	Subsequently, acute toxicity test was applied to evaluate the toxicity difference between TG and TG-n2.					
30851581	6	78	theme	TG-n2	862:866	arg1	compositions					839:850	The major chemical compositions	820:850	METHODS The major chemical compositions of TG and TG-n2	812:866	METHODS The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC).					
30851581	1	79	theme	Tripterygium	128:139	arg1	TG					153:154	TG	153:154	TG	153:154	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	1	79	theme	Tripterygium	128:139	arg1	glycosides					141:150	The Tripterygium glycosides	124:150	BACKGROUND The Tripterygium glycosides (TG)	113:155	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	11	80	theme	Acute	1348:1352	arg1	test					1363:1366	Acute toxicity test	1348:1366	Acute toxicity test	1348:1366	Acute toxicity test proved that TG-n2 was less toxic than TG.					
30851581	0	81	theme	immune	85:90	arg1	homeostasis					92:102	immune homeostasis	85:102	immune homeostasis	85:102	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.					
30851581	12	82	theme	similar	1464:1470	arg1	effect					1484:1489	a similar therapeutic effect	1462:1489	a similar therapeutic effect	1462:1489	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	5	83	from	colitis	795:801	arg1	mice					806:809	mice	806:809	mice	806:809	In this study, we aimed to investigate the difference in the chemical compositions between TG and TG-n2, further explored its toxicity and therapeutic effects on DSS-induced colitis in mice.					
30851581	12	84	from	Base	1410:1413	arg1	this					1418:1421	this	1418:1421	this	1418:1421	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	16	85	theme	therapeutic	2034:2044	arg1	effect					2046:2051	the similar therapeutic effect	2022:2051	the similar therapeutic effect	2022:2051	Moreover, the attenuated TG-n2 has the similar therapeutic effect on treating experimental colitis in mice when compared to TG.					
30851581	1	86	theme	Tripterygium	190:201	arg1	F					218:218	Tripterygium wilfordii Hook F	190:218	Tripterygium wilfordii Hook F	190:218	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	0	87	theme	glycoside	13:21	arg1	n2					32:33	Tripterygium glycoside fraction n2	0:33	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.	0:111	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.					
30851581	2	88	theme	many	405:408	arg1	diseases					410:417	many diseases	405:417	many diseases	405:417	However, due to its severe toxicity, TG is restricted to the treatment of many diseases.					
30851581	17	89	theme	Bax	2176:2178	arg1	expression					2162:2171	the expression	2158:2171	the expression of Bax in T cells	2158:2189	Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.					
30851581	14	90	theme	T	1897:1897	arg1	cells					1899:1903	CD3-positive T cells	1884:1903	CD3-positive T cells	1884:1903	Further investigated showed that TG and TG-n2 could increase the expressions of Bax and p62 on CD3-positive T cells.					
30851581	16	91	theme	experimental	2065:2076	arg1	colitis					2078:2084	experimental colitis	2065:2084	experimental colitis in mice when compared to TG	2065:2112	Moreover, the attenuated TG-n2 has the similar therapeutic effect on treating experimental colitis in mice when compared to TG.					
30851581	2	92	theme	severe	351:356	arg1	toxicity					358:365	its severe toxicity	347:365	its severe toxicity	347:365	However, due to its severe toxicity, TG is restricted to the treatment of many diseases.					
30851581	9	93	theme	TG-n2	1246:1250	arg1	different					1255:1263	different	1255:1263	different	1255:1263	RESULTS We found that the chemical compositions of TG-n2 is different from TG.					
30851581	9	93	theme	TG-n2	1246:1250	arg1	compositions					1230:1241	the chemical compositions	1217:1241	the chemical compositions of TG-n2	1217:1250	RESULTS We found that the chemical compositions of TG-n2 is different from TG.					
30851581	12	94	theme	therapeutic	1472:1482	arg1	effect					1484:1489	a similar therapeutic effect	1462:1489	a similar therapeutic effect	1462:1489	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	17	95	theme	T	2183:2183	arg1	cells					2185:2189	T cells	2183:2189	T cells	2183:2189	Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.					
30851581	0	96	theme	DSS-induced	51:61	arg1	colitis					63:69	DSS-induced colitis	51:69	DSS-induced colitis	51:69	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.					
30851581	1	97	used	used	234:237	arg2	BACKGROUND					113:122	BACKGROUND	113:122	BACKGROUND The Tripterygium glycosides (TG)	113:155	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	1	97	used	used	234:237	arg2	extractive					176:185	the main active extractive	160:185	the main active extractive of Tripterygium wilfordii Hook F	160:218	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	17	98	theme	T	2204:2204	arg1	cells					2206:2210	T cells	2204:2210	T cells	2204:2210	Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.					
30851581	1	99	theme	bowel	315:319	arg1	disease					321:327	inflammatory bowel disease	302:327	inflammatory bowel disease	302:327	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
30851581	12	100	theme	absolute	1688:1695	arg1	number					1703:1708	absolute lymph number	1688:1708	absolute lymph number	1688:1708	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	0	101	dep	n2	32:33	arg1	Alleviation					36:46	Alleviation	36:46	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.	0:111	Tripterygium glycoside fraction n2: Alleviation of DSS-induced colitis by modulating immune homeostasis in mice.					
30851581	17	102	theme	colitis	2267:2273	arg1	mice					2275:2278	colitis mice	2267:2278	colitis mice	2267:2278	Its mechanism may be related to activating the expression of Bax in T cells and inducing T cells autophagy to regulate the survival of T lymphocytes in colitis mice, thus reducing inflammation in colon.					
30851581	6	103	dep	METHODS	812:818	arg1	compositions					839:850	The major chemical compositions	820:850	METHODS The major chemical compositions of TG and TG-n2	812:866	METHODS The major chemical compositions of TG and TG-n2 were analyzed by ultra-performance liquid chromatography (UPLC).					
30851581	5	104	from	effects	772:778	arg1	colitis					795:801	DSS-induced colitis	783:801	DSS-induced colitis in mice	783:809	In this study, we aimed to investigate the difference in the chemical compositions between TG and TG-n2, further explored its toxicity and therapeutic effects on DSS-induced colitis in mice.					
30851581	12	105	theme	colonic	1600:1606	arg1	diarrhea					1557:1564	diarrhea	1557:1564	diarrhea	1557:1564	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	105	theme	colonic	1600:1606	arg1	atrophy					1608:1614	colonic atrophy	1600:1614	colonic atrophy	1600:1614	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	12	105	theme	colonic	1600:1606	arg1	symptoms					1528:1535	the symptoms	1524:1535	the symptoms	1524:1535	Base on this, further studies showed that TG-n2 has a similar therapeutic effect as compared to TG on attenuating the symptoms of colitis, such as diarrhea, bloody stools, body weight loss, colonic atrophy, histopathological changes, inhibiting cytokines secretion and reducing absolute lymph number.					
30851581	1	106	dep	Tripterygium	190:201	arg1	wilfordii					203:211	wilfordii	203:211	wilfordii	203:211	BACKGROUND The Tripterygium glycosides (TG) is the main active extractive of Tripterygium wilfordii Hook F and is widely used in clinical practice to treat inflammatory diseases (including inflammatory bowel disease).					
31491490	8	0	theme	statistical	1117:1127	arg1	spectroscopy					1129:1140	statistical spectroscopy	1117:1140	statistical spectroscopy	1117:1140	Metabolites were identified using 2D NMR spectra, statistical spectroscopy and by comparison to entries in published databases.					
31491490	11	1	dep	hip	1548:1550	arg1	SF					1561:1562	SF	1561:1562	SF	1561:1562	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	13	2	theme	factors	1814:1820	arg1	combination					1773:1783	a combination	1771:1783	a combination of intra and extra-articular factors	1771:1820	These findings may represent a combination of intra and extra-articular factors.					
31491490	0	3	theme	profiles	148:155	arg1	study					129:133	a nuclear magnetic resonance (NMR) spectroscopy study	81:133	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.	0:156	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	11	4	theme	metabolic	1527:1535	arg1	profile					1537:1543	the metabolic profile	1523:1543	the metabolic profile of hip and knee SF	1523:1562	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	10	5	dep	group	1385:1389	arg1	citrate					1436:1442	citrate	1436:1442	citrate	1436:1442	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	10	5	dep	group	1385:1389	arg1	glycosaminoglycans					1416:1433	glycosaminoglycans	1416:1433	glycosaminoglycans	1416:1433	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	10	5	dep	group	1385:1389	arg1	molecules					1405:1413	N-acetylated molecules	1392:1413	N-acetylated molecules	1392:1413	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	10	5	dep	group	1385:1389	arg1	glutamine					1448:1456	glutamine	1448:1456	glutamine	1448:1456	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	1	6	theme	contact	227:233	arg1	stresses					235:242	contact stresses	227:242	contact stresses	227:242	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	9	7	from	differences	1226:1236	arg1	profile					1255:1261	the metabolic profile	1241:1261	the metabolic profile	1241:1261	RESULTS There were significant differences in the metabolic profile between the groups.					
31491490	3	8	from	differences	458:468	arg1	metabolites					504:514	osteoarthritic hip and knee SF metabolites	473:514	osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy	473:591	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	3	9	theme	knee	496:499	arg1	metabolites					504:514	osteoarthritic hip and knee SF metabolites	473:514	osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy	473:591	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	6	10	theme	NMR	829:831	arg1	spectroscopy					833:844	NMR spectroscopy	829:844	NMR spectroscopy	829:844	NMR spectroscopy was used to analyse the metabolites present in each sample.					
31491490	3	11	theme	hip	488:490	arg1	metabolites					504:514	osteoarthritic hip and knee SF metabolites	473:514	osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy	473:591	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	10	12	theme	N-acetylated	1392:1403	arg1	citrate					1436:1442	citrate	1436:1442	citrate	1436:1442	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	10	12	theme	N-acetylated	1392:1403	arg1	glycosaminoglycans					1416:1433	glycosaminoglycans	1416:1433	glycosaminoglycans	1416:1433	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	10	12	theme	N-acetylated	1392:1403	arg1	molecules					1405:1413	N-acetylated molecules	1392:1413	N-acetylated molecules	1392:1413	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	10	12	theme	N-acetylated	1392:1403	arg1	glutamine					1448:1456	glutamine	1448:1456	glutamine	1448:1456	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	3	13	theme	metabolic	522:530	arg1	profiling					532:540	metabolic profiling	522:540	metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy	522:591	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	5	14	theme	medical	806:812	arg1	comorbidities					814:826	similar medical comorbidities	798:826	similar medical comorbidities	798:826	Samples were matched for age, gender, ethnicity and had similar medical comorbidities.					
31491490	8	15	theme	published	1174:1182	arg1	databases					1184:1192	published databases	1174:1192	published databases	1174:1192	Metabolites were identified using 2D NMR spectra, statistical spectroscopy and by comparison to entries in published databases.					
31491490	12	16	theme	diseased	1725:1732	arg1	joints					1734:1739	diseased joints	1725:1739	diseased joints	1725:1739	The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints.					
31491490	0	17	theme	resonance	100:108	arg1	study					129:133	a nuclear magnetic resonance (NMR) spectroscopy study	81:133	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.	0:156	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	7	18	theme	metabolic	1025:1033	arg1	differences					1035:1045	metabolic differences	1025:1045	metabolic differences between the groups	1025:1064	Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups.					
31491490	3	19	theme	Magnetic	555:562	arg1	NMR					575:577	NMR	575:577	NMR	575:577	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	3	19	theme	Magnetic	555:562	arg1	Resonance					564:572	Nuclear Magnetic Resonance	547:572	Nuclear Magnetic Resonance (NMR) spectroscopy	547:591	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	7	20	theme	Squares	964:970	arg1	Analysis					985:992	Orthogonal Partial Least Squares Discriminant Analysis	939:992	Orthogonal Partial Least Squares Discriminant Analysis	939:992	Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups.					
31491490	6	21	theme	present	882:888	arg1	metabolites					870:880	the metabolites	866:880	the metabolites present in each sample	866:903	NMR spectroscopy was used to analyse the metabolites present in each sample.					
31491490	12	22	from	cycle	1691:1695	arg1	joints					1734:1739	diseased joints	1725:1739	diseased joints	1725:1739	The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints.					
31491490	0	23	theme	spectroscopy	116:127	arg1	study					129:133	a nuclear magnetic resonance (NMR) spectroscopy study	81:133	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.	0:156	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	7	24	theme	Partial	950:956	arg1	Squares					964:970	Partial Least Squares	950:970	Orthogonal Partial Least Squares Discriminant Analysis	939:992	Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups.					
31491490	11	25	theme	first	1484:1488	arg1	This					1472:1475	This	1472:1475	This	1472:1475	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	11	25	theme	first	1484:1488	arg1	study					1490:1494	the first study	1480:1494	the first study	1480:1494	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	12	26	theme	oxidative	1701:1709	arg1	metabolism					1711:1720	oxidative metabolism	1701:1720	oxidative metabolism	1701:1720	The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints.					
31491490	0	27	theme	synovial	47:54	arg1	fluid					56:60	synovial fluid	47:60	synovial fluid	47:60	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	7	28	theme	Component	916:924	arg1	Analysis					926:933	Principal Component Analysis	906:933	Principal Component Analysis	906:933	Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups.					
31491490	9	29	dep	RESULTS	1195:1201	arg1	were					1209:1212	were	1209:1212	were significant differences in the metabolic profile between the groups	1209:1280	RESULTS There were significant differences in the metabolic profile between the groups.					
31491490	11	30	dep	CONCLUSIONS	1460:1470	arg1	This					1472:1475	This	1472:1475	This	1472:1475	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	11	30	dep	CONCLUSIONS	1460:1470	arg1	study					1490:1494	the first study	1480:1494	the first study	1480:1494	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	12	31	theme	tricarboxylic	1672:1684	arg1	cycle					1691:1695	the tricarboxylic acid cycle	1668:1695	the tricarboxylic acid cycle	1668:1695	The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints.					
31491490	0	32	from	Differences	0:10	arg1	composition					19:29	the composition	15:29	the composition of hip and knee synovial fluid in osteoarthritis	15:78	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	6	33	used	used	850:853	arg2	spectroscopy					833:844	NMR spectroscopy	829:844	NMR spectroscopy	829:844	NMR spectroscopy was used to analyse the metabolites present in each sample.					
31491490	0	34	theme	nuclear	83:89	arg1	NMR					111:113	NMR	111:113	NMR	111:113	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	0	34	theme	nuclear	83:89	arg1	resonance					100:108	a nuclear magnetic resonance	81:108	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.	0:156	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	11	35	from	differences	1508:1518	arg1	ESOA					1567:1570	ESOA	1567:1570	ESOA	1567:1570	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	11	35	from	differences	1508:1518	arg1	profile					1537:1543	the metabolic profile	1523:1543	the metabolic profile of hip and knee SF	1523:1562	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	12	36	theme	collagen	1646:1653	arg1	degradation					1655:1665	collagen degradation	1646:1665	collagen degradation	1646:1665	The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints.					
31491490	1	37	theme	fluid	245:249	arg1	lubrication					251:261	fluid lubrication	245:261	fluid lubrication	245:261	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	10	38	from	quantities	1336:1345	arg1	group					1359:1363	the knee group	1350:1363	the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine)	1350:1457	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	6	39	from	sample	898:903	arg1	present					882:888	present	882:888	present	882:888	NMR spectroscopy was used to analyse the metabolites present in each sample.					
31491490	1	40	theme	patterns	272:279	arg1	terms					218:222	terms	218:222	terms of contact stresses, fluid lubrication and wear patterns	218:279	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	12	41	from	metabolism	1711:1720	arg1	joints					1734:1739	diseased joints	1725:1739	diseased joints	1725:1739	The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints.					
31491490	2	42	theme	differences	399:409	arg1	nature					383:388	the nature	379:388	the nature of these differences	379:409	These differences may be reflected in the synovial fluid (SF) composition of the two joints, but the nature of these differences remains unknown.					
31491490	0	43	from	composition	19:29	arg1	osteoarthritis					65:78	osteoarthritis	65:78	osteoarthritis	65:78	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	13	44	theme	intra	1788:1792	arg1	combination					1773:1783	a combination	1771:1783	a combination of intra and extra-articular factors	1771:1820	These findings may represent a combination of intra and extra-articular factors.					
31491490	1	45	dep	hip	172:174	arg1	The					168:170	The	168:170	The	168:170	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	8	46	theme	NMR	1104:1106	arg1	spectra					1108:1114	2D NMR spectra	1101:1114	2D NMR spectra	1101:1114	Metabolites were identified using 2D NMR spectra, statistical spectroscopy and by comparison to entries in published databases.					
31491490	0	47	dep	Differences	0:10	arg1	study					129:133	a nuclear magnetic resonance (NMR) spectroscopy study	81:133	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.	0:156	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	13	48	theme	extra-articular	1798:1812	arg1	factors					1814:1820	extra-articular factors	1798:1820	extra-articular factors	1798:1820	These findings may represent a combination of intra and extra-articular factors.					
31491490	2	49	theme	synovial	324:331	arg1	SF					340:341	SF	340:341	SF	340:341	These differences may be reflected in the synovial fluid (SF) composition of the two joints, but the nature of these differences remains unknown.					
31491490	2	49	theme	synovial	324:331	arg1	fluid					333:337	synovial fluid	324:337	the synovial fluid (SF) composition of the two joints	320:372	These differences may be reflected in the synovial fluid (SF) composition of the two joints, but the nature of these differences remains unknown.					
31491490	8	50	from	entries	1163:1169	arg1	databases					1184:1192	published databases	1174:1192	published databases	1174:1192	Metabolites were identified using 2D NMR spectra, statistical spectroscopy and by comparison to entries in published databases.					
31491490	10	51	theme	hip	1381:1383	arg1	group					1385:1389	the hip group	1377:1389	the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine)	1377:1457	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	1	52	theme	stresses	235:242	arg1	terms					218:222	terms	218:222	terms of contact stresses, fluid lubrication and wear patterns	218:279	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	3	53	theme	osteoarthritic	473:486	arg1	hip					488:490	osteoarthritic hip	473:490	osteoarthritic hip	473:490	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	1	54	theme	OBJECTIVE	158:166	arg1	joints					185:190	OBJECTIVE The hip and knee joints	158:190	OBJECTIVE The hip and knee joints	158:190	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	8	55	theme	by	1146:1147	arg1	comparison					1149:1158	by comparison	1146:1158	by comparison to entries in published databases	1146:1192	Metabolites were identified using 2D NMR spectra, statistical spectroscopy and by comparison to entries in published databases.					
31491490	4	56	theme	SF	613:614	arg1	samples					616:622	DESIGN Twenty-four SF samples	594:622	DESIGN Twenty-four SF samples (12 hip, 12 knee)	594:640	DESIGN Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty.					
31491490	11	57	theme	knee	1556:1559	arg1	profile					1537:1543	the metabolic profile	1523:1543	the metabolic profile of hip and knee SF	1523:1562	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	5	58	theme	similar	798:804	arg1	comorbidities					814:826	similar medical comorbidities	798:826	similar medical comorbidities	798:826	Samples were matched for age, gender, ethnicity and had similar medical comorbidities.					
31491490	12	59	theme	identified	1577:1586	arg1	metabolites					1588:1598	The identified metabolites	1573:1598	The identified metabolites	1573:1598	The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints.					
31491490	11	60	theme	hip	1548:1550	arg1	profile					1537:1543	the metabolic profile	1523:1543	the metabolic profile of hip and knee SF	1523:1562	CONCLUSIONS This is the first study to indicate differences in the metabolic profile of hip and knee SF in ESOA.					
31491490	3	61	theme	SF	501:502	arg1	metabolites					504:514	osteoarthritic hip and knee SF metabolites	473:514	osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy	473:591	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	1	62	theme	hip	172:174	arg1	joints					185:190	OBJECTIVE The hip and knee joints	158:190	OBJECTIVE The hip and knee joints	158:190	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	9	63	theme	significant	1214:1224	arg1	differences					1226:1236	significant differences	1214:1236	significant differences in the metabolic profile between the groups	1214:1280	RESULTS There were significant differences in the metabolic profile between the groups.					
31491490	6	64	attach	present	882:888	arg1	sample					898:903	each sample	893:903	each sample	893:903	NMR spectroscopy was used to analyse the metabolites present in each sample.					
31491490	6	64	attach	present	882:888	arg2	metabolites					870:880	the metabolites	866:880	the metabolites present in each sample	866:903	NMR spectroscopy was used to analyse the metabolites present in each sample.					
31491490	1	65	theme	knee	180:183	arg1	joints					185:190	OBJECTIVE The hip and knee joints	158:190	OBJECTIVE The hip and knee joints	158:190	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	4	66	theme	DESIGN	594:599	arg1	samples					616:622	DESIGN Twenty-four SF samples	594:622	DESIGN Twenty-four SF samples (12 hip, 12 knee)	594:640	DESIGN Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty.					
31491490	7	67	theme	Discriminant	972:983	arg1	Analysis					985:992	Orthogonal Partial Least Squares Discriminant Analysis	939:992	Orthogonal Partial Least Squares Discriminant Analysis	939:992	Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups.					
31491490	4	68	with	patients	662:669	arg1	ESOA					702:705	ESOA	702:705	ESOA	702:705	DESIGN Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty.					
31491490	4	68	with	patients	662:669	arg1	osteoarthritis					686:699	end-stage osteoarthritis	676:699	end-stage osteoarthritis (ESOA)	676:706	DESIGN Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty.					
31491490	0	69	theme	metabolic	138:146	arg1	profiles					148:155	metabolic profiles	138:155	metabolic profiles	138:155	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	9	70	theme	metabolic	1245:1253	arg1	profile					1255:1261	the metabolic profile	1241:1261	the metabolic profile	1241:1261	RESULTS There were significant differences in the metabolic profile between the groups.					
31491490	7	71	theme	Least	958:962	arg1	Squares					964:970	Partial Least Squares	950:970	Orthogonal Partial Least Squares Discriminant Analysis	939:992	Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups.					
31491490	12	72	theme	acid	1686:1689	arg1	cycle					1691:1695	the tricarboxylic acid cycle	1668:1695	the tricarboxylic acid cycle	1668:1695	The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints.					
31491490	3	73	theme	Resonance	564:572	arg1	spectroscopy					580:591	Nuclear Magnetic Resonance (NMR) spectroscopy	547:591	Nuclear Magnetic Resonance (NMR) spectroscopy	547:591	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	7	74	theme	Orthogonal	939:948	arg1	Analysis					985:992	Orthogonal Partial Least Squares Discriminant Analysis	939:992	Orthogonal Partial Least Squares Discriminant Analysis	939:992	Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups.					
31491490	10	75	located	found	1305:1309	arg1	quantities					1336:1345	significantly greater quantities	1314:1345	significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine)	1314:1457	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	10	75	located	found	1305:1309	arg2	metabolites					1288:1298	Four metabolites	1283:1298	Four metabolites	1283:1298	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	12	76	from	degradation	1655:1665	arg1	joints					1734:1739	diseased joints	1725:1739	diseased joints	1725:1739	The identified metabolites can broadly be grouped into those involved in collagen degradation, the tricarboxylic acid cycle and oxidative metabolism in diseased joints.					
31491490	3	77	with	profiling	532:540	arg1	spectroscopy					580:591	Nuclear Magnetic Resonance (NMR) spectroscopy	547:591	Nuclear Magnetic Resonance (NMR) spectroscopy	547:591	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	7	78	theme	Principal	906:914	arg1	Analysis					926:933	Principal Component Analysis	906:933	Principal Component Analysis	906:933	Principal Component Analysis and Orthogonal Partial Least Squares Discriminant Analysis were undertaken to investigate metabolic differences between the groups.					
31491490	0	79	theme	magnetic	91:98	arg1	NMR					111:113	NMR	111:113	NMR	111:113	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	0	79	theme	magnetic	91:98	arg1	resonance					100:108	a nuclear magnetic resonance	81:108	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.	0:156	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	1	80	theme	lubrication	251:261	arg1	terms					218:222	terms	218:222	terms of contact stresses, fluid lubrication and wear patterns	218:279	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	3	81	theme	Nuclear	547:553	arg1	NMR					575:577	NMR	575:577	NMR	575:577	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	3	81	theme	Nuclear	547:553	arg1	Resonance					564:572	Nuclear Magnetic Resonance	547:572	Nuclear Magnetic Resonance (NMR) spectroscopy	547:591	The objective was to identify differences in osteoarthritic hip and knee SF metabolites using metabolic profiling with Nuclear Magnetic Resonance (NMR) spectroscopy.					
31491490	4	82	theme	end-stage	676:684	arg1	ESOA					702:705	ESOA	702:705	ESOA	702:705	DESIGN Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty.					
31491490	4	82	theme	end-stage	676:684	arg1	osteoarthritis					686:699	end-stage osteoarthritis	676:699	end-stage osteoarthritis (ESOA)	676:706	DESIGN Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty.					
31491490	2	83	theme	joints	367:372	arg1	composition					344:354	the synovial fluid (SF) composition	320:354	the synovial fluid (SF) composition of the two joints	320:372	These differences may be reflected in the synovial fluid (SF) composition of the two joints, but the nature of these differences remains unknown.					
31491490	0	84	theme	hip	34:36	arg1	composition					19:29	the composition	15:29	the composition of hip and knee synovial fluid in osteoarthritis	15:78	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	1	85	theme	wear	267:270	arg1	patterns					272:279	wear patterns	267:279	wear patterns	267:279	OBJECTIVE The hip and knee joints differ biomechanically in terms of contact stresses, fluid lubrication and wear patterns.					
31491490	0	86	theme	knee	42:45	arg1	composition					19:29	the composition	15:29	the composition of hip and knee synovial fluid in osteoarthritis	15:78	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	0	87	dep	hip	34:36	arg1	fluid					56:60	synovial fluid	47:60	synovial fluid	47:60	Differences in the composition of hip and knee synovial fluid in osteoarthritis: a nuclear magnetic resonance (NMR) spectroscopy study of metabolic profiles.					
31491490	10	88	theme	greater	1328:1334	arg1	quantities					1336:1345	significantly greater quantities	1314:1345	significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine)	1314:1457	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	8	89	theme	2D	1101:1102	arg1	spectra					1108:1114	2D NMR spectra	1101:1114	2D NMR spectra	1101:1114	Metabolites were identified using 2D NMR spectra, statistical spectroscopy and by comparison to entries in published databases.					
31491490	6	90	from	present	882:888	arg1	sample					898:903	each sample	893:903	each sample	893:903	NMR spectroscopy was used to analyse the metabolites present in each sample.					
31491490	4	91	theme	hip/knee	719:726	arg1	arthroplasty					728:739	hip/knee arthroplasty	719:739	hip/knee arthroplasty	719:739	DESIGN Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty.					
31491490	4	92	dep	samples	616:622	arg1	hip					628:630	12 hip	625:630	12 hip	625:630	DESIGN Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty.					
31491490	4	92	dep	samples	616:622	arg1	knee					636:639	12 knee	633:639	12 knee	633:639	DESIGN Twenty-four SF samples (12 hip, 12 knee) were collected from patients with end-stage osteoarthritis (ESOA) undergoing hip/knee arthroplasty.					
31491490	10	93	theme	knee	1354:1357	arg1	group					1359:1363	the knee group	1350:1363	the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine)	1350:1457	Four metabolites were found in significantly greater quantities in the knee group compared to the hip group (N-acetylated molecules, glycosaminoglycans, citrate and glutamine).					
31491490	2	94	theme	fluid	333:337	arg1	composition					344:354	the synovial fluid (SF) composition	320:354	the synovial fluid (SF) composition of the two joints	320:372	These differences may be reflected in the synovial fluid (SF) composition of the two joints, but the nature of these differences remains unknown.					
31099736	8	0	theme	DNA	971:973	arg1	G+C contents					975:986	DNA G+C contents	971:986	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T	971:1059	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T were 29.2 and 30.2 mol%, respectively.					
31099736	2	1	theme	human	100:104	arg1	faeces					106:111	human faeces	100:111	human faeces	100:111	nov., isolated from human faeces.					
31099736	4	2	theme	yeast	409:413	arg1	extract					415:421	Difco yeast extract	403:421	0.5% Difco yeast extract (YBHI) agar	398:433	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	4	2	theme	yeast	409:413	arg1	YBHI					424:427	YBHI	424:427	YBHI	424:427	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	6	3	dep	saccharogumia	779:791	arg1	%					799:799	92.3 %	794:799	92.3 %	794:799	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	6	3	dep	saccharogumia	779:791	arg1	%					810:810	92.2 %	805:810	92.2 %	805:810	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	9	4	theme	novel	1105:1109	arg1	strains					1111:1117	Both novel strains	1100:1117	Both novel strains	1100:1117	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	3	5	theme	faecal	254:259	arg1	samples					261:267	faecal samples	254:267	faecal samples of healthy Korean subjects	254:294	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	9	6	theme	major	1232:1236	arg1	lipids					1244:1249	the major polar lipids	1228:1249	the major polar lipids	1228:1249	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	5	7	theme	16S	520:522	arg1	sequences					534:542	16S rRNA gene sequences	520:542	16S rRNA gene sequences	520:542	Taxonomic analyses based on 16S rRNA gene sequences distinguished the strains from other species within the family Erysipelotrichaceae.					
31099736	8	8	theme	genome	1007:1012	arg1	sequences					1014:1022	the whole genome sequences	997:1022	the whole genome sequences of strains SNUG30099T and SNUG30370T	997:1059	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T were 29.2 and 30.2 mol%, respectively.					
31099736	6	9	theme	closest	632:638	arg1	muris					703:707	Longibaculum muris	690:707	Longibaculum muris	690:707	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	6	9	theme	closest	632:638	arg1	relative					640:647	The closest relative	628:647	The closest relative of strains SNUG30099T and SNUG30370T	628:684	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	4	10	from	size	486:489	arg1	3.5-4.5×0.3-0.4 µm					464:481	3.5-4.5×0.3-0.4 µm	464:481	3.5-4.5×0.3-0.4 µm	464:481	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	9	11	theme	lipids	1244:1249	arg1	phosphatidylethanolamine					1180:1203	phosphatidylethanolamine	1180:1203	phosphatidylethanolamine	1180:1203	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	9	11	theme	lipids	1244:1249	arg1	lipids					1244:1249	the major polar lipids	1228:1249	the major polar lipids	1228:1249	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	9	11	theme	lipids	1244:1249	arg1	one					1221:1223	one	1221:1223	one	1221:1223	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	3	12	theme	Korean	280:285	arg1	subjects					287:294	healthy Korean subjects	272:294	healthy Korean subjects	272:294	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	4	13	theme	%	401:401	arg1	agar					430:433	0.5% Difco yeast extract (YBHI) agar	398:433	0.5% Difco yeast extract (YBHI) agar	398:433	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	11	14	contain	have	1420:1423	arg2	characteristics					1434:1448	distinct characteristics	1425:1448	distinct characteristics	1425:1448	In addition, the profile of biochemical activities advocated that the strains have distinct characteristics in comparison to other strains.					
31099736	11	14	contain	have	1420:1423	arg1	profile					1359:1365	the profile	1355:1365	the profile of biochemical activities advocated that the strains	1355:1418	In addition, the profile of biochemical activities advocated that the strains have distinct characteristics in comparison to other strains.					
31099736	4	15	from	3.5-4.5×0.3-0.4 µm	464:481	arg1	size					486:489	size	486:489	size	486:489	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	5	16	theme	Taxonomic	492:500	arg1	analyses					502:509	Taxonomic analyses	492:509	Taxonomic analyses based on 16S rRNA gene sequences	492:542	Taxonomic analyses based on 16S rRNA gene sequences distinguished the strains from other species within the family Erysipelotrichaceae.					
31099736	5	17	from	species	581:587	arg1	strains					562:568	the strains	558:568	the strains from other species within the family Erysipelotrichaceae	558:625	Taxonomic analyses based on 16S rRNA gene sequences distinguished the strains from other species within the family Erysipelotrichaceae.					
31099736	10	18	from	those	1313:1317	arg1	composition					1282:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	10	18	from	those	1313:1317	arg1	different					1298:1306	different	1298:1306	different	1298:1306	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	12	19	theme	Faecalibacillus	1591:1605	arg1	sp					1620:1621	Faecalibacillus intestinalis sp	1591:1621	the type species Faecalibacillus intestinalis sp	1574:1621	Taken together, a novel genus, named Faecalibacillus gen. nov., is proposed, which includes the type species Faecalibacillus intestinalis sp.					
31099736	11	20	theme	biochemical	1370:1380	arg1	activities					1382:1391	biochemical activities	1370:1391	biochemical activities advocated that the strains	1370:1418	In addition, the profile of biochemical activities advocated that the strains have distinct characteristics in comparison to other strains.					
31099736	5	21	theme	other	575:579	arg1	species					581:587	other species	575:587	other species	575:587	Taxonomic analyses based on 16S rRNA gene sequences distinguished the strains from other species within the family Erysipelotrichaceae.					
31099736	10	22	theme	cellular	1262:1269	arg1	composition					1282:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	10	22	theme	cellular	1262:1269	arg1	different					1298:1306	different	1298:1306	different	1298:1306	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	5	23	theme	gene	529:532	arg1	sequences					534:542	16S rRNA gene sequences	520:542	16S rRNA gene sequences	520:542	Taxonomic analyses based on 16S rRNA gene sequences distinguished the strains from other species within the family Erysipelotrichaceae.					
31099736	15	24	theme	=KCTC	1807:1811	arg1	32257T					1824:1829	=KCTC 15632T=JCM 32257T	1807:1829	=KCTC 15632T=JCM 32257T	1807:1829	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	15	24	theme	=KCTC	1807:1811	arg1	SNUG30370T					1795:1804	SNUG30370T	1795:1804	SNUG30370T (=KCTC 15632T=JCM 32257T)	1795:1830	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	15	25	theme	15631T=JCM	1772:1781	arg1	SNUG30099T					1754:1763	SNUG30099T	1754:1763	SNUG30099T (=KCTC 15631T=JCM 32256T)	1754:1789	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	15	25	theme	15631T=JCM	1772:1781	arg1	32256T					1783:1788	=KCTC 15631T=JCM 32256T	1766:1788	=KCTC 15631T=JCM 32256T	1766:1788	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	14	26	theme	strain	1691:1696	arg1	SNUG30370T					1698:1707	strain SNUG30370T	1691:1707	strain SNUG30370T	1691:1707	nov. for strain SNUG30370T.					
31099736	12	27	theme	type	1578:1581	arg1	species					1583:1589	the type species	1574:1589	the type species Faecalibacillus intestinalis sp	1574:1621	Taken together, a novel genus, named Faecalibacillus gen. nov., is proposed, which includes the type species Faecalibacillus intestinalis sp.					
31099736	3	28	theme	long-rod-shaped	118:132	arg1	strains					199:205	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains	114:205	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains	114:205	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	3	28	theme	long-rod-shaped	118:132	arg1	SNUG30370T					223:232	SNUG30370T	223:232	SNUG30370T	223:232	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	3	28	theme	long-rod-shaped	118:132	arg1	SNUG30099T					208:217	SNUG30099T	208:217	SNUG30099T	208:217	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	10	29	theme	acid	1277:1280	arg1	composition					1282:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	10	29	theme	acid	1277:1280	arg1	different					1298:1306	different	1298:1306	different	1298:1306	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	1	30	theme	Faecalibacillus	53:67	arg1	faecis					69:74	nov. and Faecalibacillus faecis	44:74	nov. and Faecalibacillus faecis	44:74	nov. and Faecalibacillus faecis sp.					
31099736	15	31	theme	=KCTC	1766:1770	arg1	SNUG30099T					1754:1763	SNUG30099T	1754:1763	SNUG30099T (=KCTC 15631T=JCM 32256T)	1754:1789	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	15	31	theme	=KCTC	1766:1770	arg1	32256T					1783:1788	=KCTC 15631T=JCM 32256T	1766:1788	=KCTC 15631T=JCM 32256T	1766:1788	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	9	32	contain	possessed	1119:1127	arg2	acid					1149:1152	meso-diaminopimelic acid	1129:1152	meso-diaminopimelic acid	1129:1152	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	9	32	contain	possessed	1119:1127	arg1	strains					1111:1117	Both novel strains	1100:1117	Both novel strains	1100:1117	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	9	32	contain	possessed	1119:1127	arg2	peptidoglycan					1161:1173	the peptidoglycan	1157:1173	the peptidoglycan	1157:1173	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	8	33	theme	29.2 and	1066:1073	arg1	%					1083:1083	29.2 and 30.2 mol%	1066:1083	29.2 and 30.2 mol%	1066:1083	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T were 29.2 and 30.2 mol%, respectively.					
31099736	6	34	theme	93.6 	721:725	arg1	%					726:726	%	726:726	%	726:726	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	0	35	theme	Faecalibacillus	0:14	arg1	sp					40:41	sp	40:41	sp	40:41	Faecalibacillus intestinalis gen. nov., sp.					
31099736	0	35	theme	Faecalibacillus	0:14	arg1	nov.					34:37	Faecalibacillus intestinalis gen. nov.	0:37	Faecalibacillus intestinalis gen. nov.	0:37	Faecalibacillus intestinalis gen. nov., sp.					
31099736	5	36	theme	family	600:605	arg1	Erysipelotrichaceae					607:625	the family Erysipelotrichaceae	596:625	the family Erysipelotrichaceae	596:625	Taxonomic analyses based on 16S rRNA gene sequences distinguished the strains from other species within the family Erysipelotrichaceae.					
31099736	3	37	dep	long-rod-shaped	118:132	arg1	anaerobic					167:175	anaerobic	167:175	anaerobic	167:175	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	3	37	dep	long-rod-shaped	118:132	arg1	non-spore-forming					181:197	non-spore-forming	181:197	non-spore-forming	181:197	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	3	37	dep	long-rod-shaped	118:132	arg1	Gram-stain-positive					135:153	Gram-stain-positive	135:153	Gram-stain-positive	135:153	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	0	38	theme	gen.	29:32	arg1	sp					40:41	sp	40:41	sp	40:41	Faecalibacillus intestinalis gen. nov., sp.					
31099736	0	38	theme	gen.	29:32	arg1	nov.					34:37	Faecalibacillus intestinalis gen. nov.	0:37	Faecalibacillus intestinalis gen. nov.	0:37	Faecalibacillus intestinalis gen. nov., sp.					
31099736	12	39	theme	gen.	1535:1538	arg1	nov.					1540:1543	Faecalibacillus gen. nov.	1519:1543	Faecalibacillus gen. nov.	1519:1543	Taken together, a novel genus, named Faecalibacillus gen. nov., is proposed, which includes the type species Faecalibacillus intestinalis sp.					
31099736	10	40	theme	related	1328:1334	arg1	taxa					1336:1339	other related taxa	1322:1339	other related taxa	1322:1339	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	4	41	theme	infusion	364:371	arg1	medium					373:378	Brain-heart infusion medium	352:378	Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar	352:433	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	13	42	dep	SNUG30099T	1640:1649	arg1	sp					1678:1679	faecis sp	1671:1679	faecis sp	1671:1679	nov. for strain SNUG30099T and Faecalibacillus faecis sp.					
31099736	13	42	dep	SNUG30099T	1640:1649	arg1	nov.					1624:1627	nov.	1624:1627	nov. for strain	1624:1638	nov. for strain SNUG30099T and Faecalibacillus faecis sp.					
31099736	13	43	theme	faecis	1671:1676	arg1	sp					1678:1679	faecis sp	1671:1679	faecis sp	1671:1679	nov. for strain SNUG30099T and Faecalibacillus faecis sp.					
31099736	4	44	theme	ivory-coloured	325:338	arg1	colonies					340:347	circular ivory-coloured colonies	316:347	circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar	316:433	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	7	45	theme	related	908:914	arg1	strains					916:922	other related strains	902:922	other related strains that belong to the family Erysipelotrichaceae	902:968	Phylogenetic inference also divided the strains into a unique branch that differed from other related strains that belong to the family Erysipelotrichaceae.					
31099736	1	46	theme	nov.	44:47	arg1	faecis					69:74	nov. and Faecalibacillus faecis	44:74	nov. and Faecalibacillus faecis	44:74	nov. and Faecalibacillus faecis sp.					
31099736	3	47	attach	isolated	240:247	arg2	SNUG30099T					208:217	SNUG30099T	208:217	SNUG30099T	208:217	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	3	47	attach	isolated	240:247	arg2	strains					199:205	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains	114:205	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains	114:205	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	3	47	attach	isolated	240:247	arg2	SNUG30370T					223:232	SNUG30370T	223:232	SNUG30370T	223:232	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	3	47	attach	isolated	240:247	arg1	samples					261:267	faecal samples	254:267	faecal samples of healthy Korean subjects	254:294	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	4	48	theme	extract	415:421	arg1	agar					430:433	0.5% Difco yeast extract (YBHI) agar	398:433	0.5% Difco yeast extract (YBHI) agar	398:433	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	9	49	theme	polar	1238:1242	arg1	lipids					1244:1249	the major polar lipids	1228:1249	the major polar lipids	1228:1249	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	15	50	theme	novel	1736:1740	arg1	species					1742:1748	these novel species	1730:1748	these novel species	1730:1748	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	8	51	theme	whole	1001:1005	arg1	sequences					1014:1022	the whole genome sequences	997:1022	the whole genome sequences of strains SNUG30099T and SNUG30370T	997:1059	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T were 29.2 and 30.2 mol%, respectively.					
31099736	11	52	theme	distinct	1425:1432	arg1	characteristics					1434:1448	distinct characteristics	1425:1448	distinct characteristics	1425:1448	In addition, the profile of biochemical activities advocated that the strains have distinct characteristics in comparison to other strains.					
31099736	7	53	theme	unique	869:874	arg1	branch					876:881	a unique branch	867:881	a unique branch that differed from other related strains that belong to the family Erysipelotrichaceae	867:968	Phylogenetic inference also divided the strains into a unique branch that differed from other related strains that belong to the family Erysipelotrichaceae.					
31099736	4	54	theme	0.5	398:400	arg1	%					401:401	%	401:401	%	401:401	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	15	55	theme	type	1714:1717	arg1	SNUG30099T					1754:1763	SNUG30099T	1754:1763	SNUG30099T (=KCTC 15631T=JCM 32256T)	1754:1789	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	15	55	theme	type	1714:1717	arg1	strains					1719:1725	The type strains	1710:1725	The type strains of these novel species	1710:1748	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	5	56	theme	rRNA	524:527	arg1	sequences					534:542	16S rRNA gene sequences	520:542	16S rRNA gene sequences	520:542	Taxonomic analyses based on 16S rRNA gene sequences distinguished the strains from other species within the family Erysipelotrichaceae.					
31099736	4	57	theme	Difco	403:407	arg1	extract					415:421	Difco yeast extract	403:421	0.5% Difco yeast extract (YBHI) agar	398:433	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	4	57	theme	Difco	403:407	arg1	YBHI					424:427	YBHI	424:427	YBHI	424:427	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	8	58	theme	strains	1027:1033	arg1	sequences					1014:1022	the whole genome sequences	997:1022	the whole genome sequences of strains SNUG30099T and SNUG30370T	997:1059	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T were 29.2 and 30.2 mol%, respectively.					
31099736	3	59	theme	subjects	287:294	arg1	samples					261:267	faecal samples	254:267	faecal samples of healthy Korean subjects	254:294	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	11	60	theme	activities	1382:1391	arg1	profile					1359:1365	the profile	1355:1365	the profile of biochemical activities advocated that the strains	1355:1418	In addition, the profile of biochemical activities advocated that the strains have distinct characteristics in comparison to other strains.					
31099736	15	61	theme	species	1742:1748	arg1	SNUG30099T					1754:1763	SNUG30099T	1754:1763	SNUG30099T (=KCTC 15631T=JCM 32256T)	1754:1789	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	15	61	theme	species	1742:1748	arg1	strains					1719:1725	The type strains	1710:1725	The type strains of these novel species	1710:1748	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	12	62	theme	intestinalis	1607:1618	arg1	sp					1620:1621	Faecalibacillus intestinalis sp	1591:1621	the type species Faecalibacillus intestinalis sp	1574:1621	Taken together, a novel genus, named Faecalibacillus gen. nov., is proposed, which includes the type species Faecalibacillus intestinalis sp.					
31099736	3	63	theme	healthy	272:278	arg1	subjects					287:294	healthy Korean subjects	272:294	healthy Korean subjects	272:294	Two long-rod-shaped, Gram-stain-positive, obligately anaerobic and non-spore-forming strains, SNUG30099T and SNUG30370T, were isolated from faecal samples of healthy Korean subjects.					
31099736	6	64	dep	strains	652:658	arg1	SNUG30099T					660:669	SNUG30099T	660:669	SNUG30099T	660:669	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	6	64	dep	strains	652:658	arg1	strains					652:658	strains SNUG30099T and SNUG30370T	652:684	strains SNUG30099T and SNUG30370T	652:684	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	6	64	dep	strains	652:658	arg1	SNUG30370T					675:684	SNUG30370T	675:684	SNUG30370T	675:684	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	1	65	dep	sp	76:77	arg1	faecis					69:74	nov. and Faecalibacillus faecis	44:74	nov. and Faecalibacillus faecis	44:74	nov. and Faecalibacillus faecis sp.					
31099736	10	66	theme	fatty	1271:1275	arg1	composition					1282:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	10	66	theme	fatty	1271:1275	arg1	different					1298:1306	different	1298:1306	different	1298:1306	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	4	67	from	colonies	340:347	arg1	medium					373:378	Brain-heart infusion medium	352:378	Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar	352:433	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	10	68	theme	major	1256:1260	arg1	composition					1282:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition	1252:1292	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	10	68	theme	major	1256:1260	arg1	different					1298:1306	different	1298:1306	different	1298:1306	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	6	69	dep	muris	703:707	arg1	%					715:715	92.9 %	710:715	92.9 %	710:715	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	6	69	dep	muris	703:707	arg1	followed					755:762	followed	755:762	followed by Clostridium saccharogumia (92.3 % and 92.2 %)	755:811	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	6	69	dep	muris	703:707	arg1	similarity					728:737	93.6 % similarity	721:737	93.6 % similarity	721:737	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	6	70	theme	strains	652:658	arg1	muris					703:707	Longibaculum muris	690:707	Longibaculum muris	690:707	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	6	70	theme	strains	652:658	arg1	relative					640:647	The closest relative	628:647	The closest relative of strains SNUG30099T and SNUG30370T	628:684	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	8	71	dep	strains	1027:1033	arg1	SNUG30370T					1050:1059	SNUG30370T	1050:1059	SNUG30370T	1050:1059	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T were 29.2 and 30.2 mol%, respectively.					
31099736	8	71	dep	strains	1027:1033	arg1	SNUG30099T					1035:1044	SNUG30099T	1035:1044	SNUG30099T	1035:1044	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T were 29.2 and 30.2 mol%, respectively.					
31099736	8	71	dep	strains	1027:1033	arg1	strains					1027:1033	strains SNUG30099T and SNUG30370T	1027:1059	strains SNUG30099T and SNUG30370T	1027:1059	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T were 29.2 and 30.2 mol%, respectively.					
31099736	10	72	theme	other	1322:1326	arg1	taxa					1336:1339	other related taxa	1322:1339	other related taxa	1322:1339	The major cellular fatty acid composition was different from those of other related taxa.					
31099736	8	73	theme	30.2 mol	1075:1082	arg1	%					1083:1083	29.2 and 30.2 mol%	1066:1083	29.2 and 30.2 mol%	1066:1083	DNA G+C contents based on the whole genome sequences of strains SNUG30099T and SNUG30370T were 29.2 and 30.2 mol%, respectively.					
31099736	15	74	theme	15632T=JCM	1813:1822	arg1	32257T					1824:1829	=KCTC 15632T=JCM 32257T	1807:1829	=KCTC 15632T=JCM 32257T	1807:1829	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	15	74	theme	15632T=JCM	1813:1822	arg1	SNUG30370T					1795:1804	SNUG30370T	1795:1804	SNUG30370T (=KCTC 15632T=JCM 32257T)	1795:1830	The type strains of these novel species are SNUG30099T (=KCTC 15631T=JCM 32256T) and SNUG30370T (=KCTC 15632T=JCM 32257T).					
31099736	0	75	theme	intestinalis	16:27	arg1	sp					40:41	sp	40:41	sp	40:41	Faecalibacillus intestinalis gen. nov., sp.					
31099736	0	75	theme	intestinalis	16:27	arg1	nov.					34:37	Faecalibacillus intestinalis gen. nov.	0:37	Faecalibacillus intestinalis gen. nov.	0:37	Faecalibacillus intestinalis gen. nov., sp.					
31099736	7	76	theme	family	943:948	arg1	Erysipelotrichaceae					950:968	the family Erysipelotrichaceae	939:968	the family Erysipelotrichaceae	939:968	Phylogenetic inference also divided the strains into a unique branch that differed from other related strains that belong to the family Erysipelotrichaceae.					
31099736	7	77	theme	Phylogenetic	814:825	arg1	inference					827:835	Phylogenetic inference	814:835	Phylogenetic inference	814:835	Phylogenetic inference also divided the strains into a unique branch that differed from other related strains that belong to the family Erysipelotrichaceae.					
31099736	9	78	theme	meso-diaminopimelic	1129:1147	arg1	peptidoglycan					1161:1173	the peptidoglycan	1157:1173	the peptidoglycan	1157:1173	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	9	78	theme	meso-diaminopimelic	1129:1147	arg1	acid					1149:1152	meso-diaminopimelic acid	1129:1152	meso-diaminopimelic acid	1129:1152	Both novel strains possessed meso-diaminopimelic acid as the peptidoglycan, and phosphatidylethanolamine was observed as one of the major polar lipids.					
31099736	12	79	theme	Faecalibacillus	1519:1533	arg1	nov.					1540:1543	Faecalibacillus gen. nov.	1519:1543	Faecalibacillus gen. nov.	1519:1543	Taken together, a novel genus, named Faecalibacillus gen. nov., is proposed, which includes the type species Faecalibacillus intestinalis sp.					
31099736	4	80	theme	Brain-heart	352:362	arg1	medium					373:378	Brain-heart infusion medium	352:378	Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar	352:433	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31099736	11	81	theme	other	1467:1471	arg1	strains					1473:1479	other strains	1467:1479	other strains	1467:1479	In addition, the profile of biochemical activities advocated that the strains have distinct characteristics in comparison to other strains.					
31099736	12	82	dep	species	1583:1589	arg1	sp					1620:1621	Faecalibacillus intestinalis sp	1591:1621	the type species Faecalibacillus intestinalis sp	1574:1621	Taken together, a novel genus, named Faecalibacillus gen. nov., is proposed, which includes the type species Faecalibacillus intestinalis sp.					
31099736	6	83	theme	Clostridium	767:777	arg1	saccharogumia					779:791	Clostridium saccharogumia	767:791	Clostridium saccharogumia (92.3 % and 92.2 %)	767:811	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	12	84	theme	novel	1500:1504	arg1	genus					1506:1510	a novel genus	1498:1510	a novel genus	1498:1510	Taken together, a novel genus, named Faecalibacillus gen. nov., is proposed, which includes the type species Faecalibacillus intestinalis sp.					
31099736	7	85	theme	other	902:906	arg1	strains					916:922	other related strains	902:922	other related strains that belong to the family Erysipelotrichaceae	902:968	Phylogenetic inference also divided the strains into a unique branch that differed from other related strains that belong to the family Erysipelotrichaceae.					
31099736	6	86	theme	%	726:726	arg1	similarity					728:737	93.6 % similarity	721:737	93.6 % similarity	721:737	The closest relative of strains SNUG30099T and SNUG30370T was Longibaculum muris (92.9 % and 93.6 % similarity, respectively), followed by Clostridium saccharogumia (92.3 % and 92.2 %).					
31099736	4	87	theme	circular	316:323	arg1	colonies					340:347	circular ivory-coloured colonies	316:347	circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar	316:433	The strains formed circular ivory-coloured colonies on Brain-heart infusion medium supplemented with 0.5% Difco yeast extract (YBHI) agar and cells were approximately 3.5-4.5×0.3-0.4 µm in size.					
31744758	3	0	theme	nanoparticles	540:552	arg1	size					532:535	The size	528:535	The size of nanoparticles	528:552	The size of nanoparticles was about 100-150 nm with well-ordered mesoporous structure and PNIPAM chains coating on the surface as outer shell.					
31744758	3	0	theme	nanoparticles	540:552	arg1	100-150 nm					564:573	100-150 nm	564:573	100-150 nm	564:573	The size of nanoparticles was about 100-150 nm with well-ordered mesoporous structure and PNIPAM chains coating on the surface as outer shell.					
31744758	6	1	theme	release	928:934	arg1	kinetics					936:943	The release kinetics	924:943	The release kinetics	924:943	The release kinetics were studied using different models to explain drug release mechanism.					
31744758	1	2	theme	β-CD-PNIPAM	285:295	arg1	polymer					310:316	ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer	191:316	polymer	310:316	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	0	3	theme	drug	92:95	arg1	release					97:103	controlled drug release	81:103	controlled drug release	81:103	Temperature and H2O2-operated nano-valves on mesoporous silica nanoparticles for controlled drug release and kinetics.					
31744758	1	4	theme	ferrocene	191:199	arg1	MSN-Fc					229:234	ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer	191:316	MSN-Fc	229:234	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	0	5	from	Temperature	0:10	arg1	silica					56:61	mesoporous silica	45:61	mesoporous silica	45:61	Temperature and H2O2-operated nano-valves on mesoporous silica nanoparticles for controlled drug release and kinetics.					
31744758	3	6	theme	mesoporous	593:602	arg1	structure					604:612	well-ordered mesoporous structure	580:612	well-ordered mesoporous structure	580:612	The size of nanoparticles was about 100-150 nm with well-ordered mesoporous structure and PNIPAM chains coating on the surface as outer shell.					
31744758	3	7	theme	PNIPAM	618:623	arg1	chains					625:630	PNIPAM chains	618:630	PNIPAM chains	618:630	The size of nanoparticles was about 100-150 nm with well-ordered mesoporous structure and PNIPAM chains coating on the surface as outer shell.					
31744758	1	8	theme	star-shaped	298:308	arg1	polymer					310:316	ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer	191:316	polymer	310:316	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	5	9	from	increase	859:866	arg1	O2					920:921	O2	920:921	O2	920:921	The release of DOX from nanoparticles was enhanced with the increase of temperature above LCST or adding H2O2 in ambient O2.					
31744758	3	10	with	100-150 nm	564:573	arg1	structure					604:612	well-ordered mesoporous structure	580:612	well-ordered mesoporous structure	580:612	The size of nanoparticles was about 100-150 nm with well-ordered mesoporous structure and PNIPAM chains coating on the surface as outer shell.					
31744758	3	10	with	100-150 nm	564:573	arg1	chains					625:630	PNIPAM chains	618:630	PNIPAM chains	618:630	The size of nanoparticles was about 100-150 nm with well-ordered mesoporous structure and PNIPAM chains coating on the surface as outer shell.					
31744758	4	11	theme	loading	763:769	arg1	capacity					771:778	the high drug loading capacity	749:778	the high drug loading capacity for nanoparticles	749:796	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	7	12	theme	drug	1033:1036	arg1	nanoparticles					1055:1067	the drug loaded composite nanoparticles	1029:1067	the drug loaded composite nanoparticles	1029:1067	Furthermore, the drug loaded composite nanoparticles exhibited excellent anti-cancer activity.					
31744758	5	13	theme	adding	897:902	arg1	H2O2					904:907	LCST or adding H2O2	889:907	H2O2	904:907	The release of DOX from nanoparticles was enhanced with the increase of temperature above LCST or adding H2O2 in ambient O2.					
31744758	2	14	theme	MSN-Fc	425:430	arg1	nanoparticles					442:454	β-CD-PNIPAM@MSN-Fc composite nanoparticles	413:454	β-CD-PNIPAM@MSN-Fc composite nanoparticles	413:454	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	1	15	theme	modified	201:208	arg1	MSN-Fc					229:234	ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer	191:316	MSN-Fc	229:234	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	3	16	theme	well-ordered	580:591	arg1	structure					604:612	well-ordered mesoporous structure	580:612	well-ordered mesoporous structure	580:612	The size of nanoparticles was about 100-150 nm with well-ordered mesoporous structure and PNIPAM chains coating on the surface as outer shell.					
31744758	1	17	theme	mesoporous	210:219	arg1	MSN-Fc					229:234	ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer	191:316	MSN-Fc	229:234	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	2	18	theme	TEM	484:486	arg1	isotherms					517:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	5	19	theme	DOX	814:816	arg1	release					803:809	The release	799:809	The release of DOX from nanoparticles	799:835	The release of DOX from nanoparticles was enhanced with the increase of temperature above LCST or adding H2O2 in ambient O2.					
31744758	4	20	theme	hydrophobic	696:706	arg1	cavities					708:715	hydrophobic cavities	696:715	hydrophobic cavities of β-CD	696:723	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	1	21	theme	silica	221:226	arg1	MSN-Fc					229:234	ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer	191:316	MSN-Fc	229:234	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	1	22	theme	due	318:320	arg1	MSN-Fc					229:234	ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer	191:316	MSN-Fc	229:234	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	6	23	theme	different	964:972	arg1	models					974:979	different models	964:979	different models	964:979	The release kinetics were studied using different models to explain drug release mechanism.					
31744758	2	24	theme	nanoparticles	442:454	arg1	structure					400:408	structure	400:408	structure	400:408	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	2	24	theme	nanoparticles	442:454	arg1	formation					386:394	formation	386:394	formation	386:394	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	2	25	theme	@	424:424	arg1	nanoparticles					442:454	β-CD-PNIPAM@MSN-Fc composite nanoparticles	413:454	β-CD-PNIPAM@MSN-Fc composite nanoparticles	413:454	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	2	26	theme	TGA	479:481	arg1	isotherms					517:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	2	27	dep	formation	386:394	arg1	The					382:384	The	382:384	The	382:384	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	2	28	theme	FTIR	473:476	arg1	isotherms					517:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	0	29	theme	H2O2-operated	16:28	arg1	nano-valves					30:40	H2O2-operated nano-valves	16:40	H2O2-operated nano-valves	16:40	Temperature and H2O2-operated nano-valves on mesoporous silica nanoparticles for controlled drug release and kinetics.					
31744758	6	30	theme	release	997:1003	arg1	mechanism					1005:1013	drug release mechanism	992:1013	drug release mechanism	992:1013	The release kinetics were studied using different models to explain drug release mechanism.					
31744758	1	31	theme	Temperature	119:129	arg1	nanoparticles					156:168	Temperature and H2O2 dual-responsive nanoparticles	119:168	Temperature and H2O2 dual-responsive nanoparticles	119:168	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	0	32	theme	mesoporous	45:54	arg1	silica					56:61	mesoporous silica	45:61	mesoporous silica	45:61	Temperature and H2O2-operated nano-valves on mesoporous silica nanoparticles for controlled drug release and kinetics.					
31744758	1	33	theme	host-guest	329:338	arg1	interactions					340:351	the host-guest interactions	325:351	the host-guest interactions for controlled drug release	325:379	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	6	34	theme	drug	992:995	arg1	mechanism					1005:1013	drug release mechanism	992:1013	drug release mechanism	992:1013	The release kinetics were studied using different models to explain drug release mechanism.					
31744758	5	35	theme	LCST	889:892	arg1	H2O2					904:907	LCST or adding H2O2	889:907	H2O2	904:907	The release of DOX from nanoparticles was enhanced with the increase of temperature above LCST or adding H2O2 in ambient O2.					
31744758	2	36	theme	adsorption-desorption	495:515	arg1	isotherms					517:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	1	37	theme	β-cyclodextrin-poly	241:259	arg1	polymer					310:316	ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer	191:316	polymer	310:316	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	2	38	theme	N2	492:493	arg1	isotherms					517:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	FTIR, TGA, TEM and N2 adsorption-desorption isotherms	473:525	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	4	39	theme	β-CD	720:723	arg1	cavities					708:715	hydrophobic cavities	696:715	hydrophobic cavities of β-CD	696:723	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	4	39	theme	β-CD	720:723	arg1	channels					675:682	The channels	671:682	The channels of MSNs	671:690	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	4	39	theme	β-CD	720:723	arg1	all					730:732	all	730:732	all	730:732	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	4	40	theme	high	753:756	arg1	capacity					771:778	the high drug loading capacity	749:778	the high drug loading capacity for nanoparticles	749:796	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	5	41	from	nanoparticles	823:835	arg1	release					803:809	The release	799:809	The release of DOX from nanoparticles	799:835	The release of DOX from nanoparticles was enhanced with the increase of temperature above LCST or adding H2O2 in ambient O2.					
31744758	1	42	theme	controlled	357:366	arg1	release					373:379	controlled drug release	357:379	controlled drug release	357:379	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	7	43	theme	loaded	1038:1043	arg1	nanoparticles					1055:1067	the drug loaded composite nanoparticles	1029:1067	the drug loaded composite nanoparticles	1029:1067	Furthermore, the drug loaded composite nanoparticles exhibited excellent anti-cancer activity.					
31744758	1	44	theme	H2O2	135:138	arg1	nanoparticles					156:168	Temperature and H2O2 dual-responsive nanoparticles	119:168	Temperature and H2O2 dual-responsive nanoparticles	119:168	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	1	45	theme	drug	368:371	arg1	release					373:379	controlled drug release	357:379	controlled drug release	357:379	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	7	46	theme	excellent	1079:1087	arg1	activity					1101:1108	excellent anti-cancer activity	1079:1108	excellent anti-cancer activity	1079:1108	Furthermore, the drug loaded composite nanoparticles exhibited excellent anti-cancer activity.					
31744758	7	47	theme	composite	1045:1053	arg1	nanoparticles					1055:1067	the drug loaded composite nanoparticles	1029:1067	the drug loaded composite nanoparticles	1029:1067	Furthermore, the drug loaded composite nanoparticles exhibited excellent anti-cancer activity.					
31744758	1	48	theme	dual-responsive	140:154	arg1	nanoparticles					156:168	Temperature and H2O2 dual-responsive nanoparticles	119:168	Temperature and H2O2 dual-responsive nanoparticles	119:168	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	7	49	theme	anti-cancer	1089:1099	arg1	activity					1101:1108	excellent anti-cancer activity	1079:1108	excellent anti-cancer activity	1079:1108	Furthermore, the drug loaded composite nanoparticles exhibited excellent anti-cancer activity.					
31744758	4	50	theme	drug	758:761	arg1	capacity					771:778	the high drug loading capacity	749:778	the high drug loading capacity for nanoparticles	749:796	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	1	51	theme	N-isopropylacrylamide	261:281	arg1	polymer					310:316	ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer	191:316	polymer	310:316	Temperature and H2O2 dual-responsive nanoparticles were fabricated from ferrocene modified mesoporous silica (MSN-Fc) and β-cyclodextrin-poly(N-isopropylacrylamide) (β-CD-PNIPAM) star-shaped polymer due to the host-guest interactions for controlled drug release.					
31744758	0	52	theme	controlled	81:90	arg1	release					97:103	controlled drug release	81:103	controlled drug release	81:103	Temperature and H2O2-operated nano-valves on mesoporous silica nanoparticles for controlled drug release and kinetics.					
31744758	4	53	theme	MSNs	687:690	arg1	cavities					708:715	hydrophobic cavities	696:715	hydrophobic cavities of β-CD	696:723	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	4	53	theme	MSNs	687:690	arg1	channels					675:682	The channels	671:682	The channels of MSNs	671:690	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	4	53	theme	MSNs	687:690	arg1	all					730:732	all	730:732	all	730:732	The channels of MSNs and hydrophobic cavities of β-CD were all contributed to the high drug loading capacity for nanoparticles.					
31744758	3	54	theme	outer	658:662	arg1	shell					664:668	outer shell	658:668	outer shell	658:668	The size of nanoparticles was about 100-150 nm with well-ordered mesoporous structure and PNIPAM chains coating on the surface as outer shell.					
31744758	5	55	theme	temperature	871:881	arg1	increase					859:866	the increase	855:866	the increase of temperature above LCST or adding H2O2 in ambient O2	855:921	The release of DOX from nanoparticles was enhanced with the increase of temperature above LCST or adding H2O2 in ambient O2.					
31744758	2	56	theme	β-CD-PNIPAM	413:423	arg1	nanoparticles					442:454	β-CD-PNIPAM@MSN-Fc composite nanoparticles	413:454	β-CD-PNIPAM@MSN-Fc composite nanoparticles	413:454	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	2	57	theme	composite	432:440	arg1	nanoparticles					442:454	β-CD-PNIPAM@MSN-Fc composite nanoparticles	413:454	β-CD-PNIPAM@MSN-Fc composite nanoparticles	413:454	The formation and structure of β-CD-PNIPAM@MSN-Fc composite nanoparticles was confirmed by FTIR, TGA, TEM and N2 adsorption-desorption isotherms.					
31744758	0	58	from	nano-valves	30:40	arg1	silica					56:61	mesoporous silica	45:61	mesoporous silica	45:61	Temperature and H2O2-operated nano-valves on mesoporous silica nanoparticles for controlled drug release and kinetics.					
30187475	0	0	theme	soybean	67:73	arg1	sprouts					75:81	yellow soybean sprouts	60:81	yellow soybean sprouts	60:81	Effect of chitosan pre-soaking on the growth and quality of yellow soybean sprouts.					
30187475	5	1	from	value	995:999	arg1	soybean					1017:1023	ungerminated soybean	1004:1023	ungerminated soybean	1004:1023	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	4	2	theme	germination	747:757	arg1	concentration					717:729	the concentration	713:729	the concentration of chitosan and germination time	713:762	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	5	3	theme	acid	884:887	arg1	1.56					936:939	1.56	936:939	1.56	936:939	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	5	3	theme	acid	884:887	arg1	content					889:895	the phytic acid content	873:895	the phytic acid content values after 5 days of germination	873:930	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	6	4	from	content	1159:1165	arg1	sprouts					1192:1198	sprouts	1192:1198	sprouts	1192:1198	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	2	5	theme	different	449:457	arg1	concentrations					459:472	different concentrations	449:472	different concentrations of chitosan solutions	449:494	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	6	6	dep	length	1085:1090	arg1	the					1071:1073	the	1071:1073	the	1071:1073	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	7	7	theme	foods	1394:1398	arg1	production					1360:1369	the production	1356:1369	the production of low-cost functional foods	1356:1398	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	1	8	from	food	137:140	arg1	countries					156:164	many Asian countries	145:164	many Asian countries	145:164	BACKGROUND Soybeans are popularly known as a healthy food in many Asian countries and are mostly consumed as sprouts.					
30187475	2	9	theme	sprouts	321:327	arg1	applications					255:266	the potential applications	241:266	the potential applications of chitosan	241:278	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	9	theme	sprouts	321:327	arg1	regulator					300:308	a natural growth regulator	283:308	a natural growth regulator of soybean sprouts	283:327	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	5	10	theme	phytic	877:882	arg1	1.56					936:939	1.56	936:939	1.56	936:939	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	5	10	theme	phytic	877:882	arg1	content					889:895	the phytic acid content	873:895	the phytic acid content values after 5 days of germination	873:930	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	0	11	from	Effect	0:5	arg1	quality					49:55	quality	49:55	quality	49:55	Effect of chitosan pre-soaking on the growth and quality of yellow soybean sprouts.					
30187475	0	11	from	Effect	0:5	arg1	growth					38:43	growth	38:43	growth	38:43	Effect of chitosan pre-soaking on the growth and quality of yellow soybean sprouts.					
30187475	7	12	theme	alternative	1329:1339	arg1	technology					1341:1350	an alternative technology	1326:1350	an alternative technology	1326:1350	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	7	12	theme	alternative	1329:1339	arg1	Chitosan					1302:1309	Chitosan pre-soaking	1302:1321	Chitosan pre-soaking	1302:1321	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	5	13	theme	g	941:941	arg1	kg-1					943:946	g kg-1	941:946	1.56 g kg-1	936:946	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	0	14	theme	sprouts	75:81	arg1	quality					49:55	quality	49:55	quality	49:55	Effect of chitosan pre-soaking on the growth and quality of yellow soybean sprouts.					
30187475	0	14	theme	sprouts	75:81	arg1	growth					38:43	growth	38:43	growth	38:43	Effect of chitosan pre-soaking on the growth and quality of yellow soybean sprouts.					
30187475	6	15	theme	sprouts	1120:1126	arg1	length					1085:1090	hypocotyl length	1075:1090	hypocotyl length	1075:1090	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	6	15	theme	sprouts	1120:1126	arg1	weight					1102:1107	fresh weight	1096:1107	fresh weight	1096:1107	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	3	16	theme	fresh	530:534	arg1	weight					536:541	fresh weight	530:541	fresh weight	530:541	RESULTS The hypocotyl length and fresh weight of the soybean sprouts could be positively affected by chitosan treatment.					
30187475	5	17	theme	soybean	852:858	arg1	sprouts					860:866	8 g kg-1 chitosan-treated soybean sprouts	826:866	8 g kg-1 chitosan-treated soybean sprouts	826:866	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	8	18	theme	Chemical	1463:1470	arg1	Industry					1472:1479	Chemical Industry	1463:1479	Chemical Industry	1463:1479	© 2018 Society of Chemical Industry.					
30187475	5	19	theme	germination	920:930	arg1	days					912:915	5 days	910:915	5 days of germination	910:930	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	4	20	theme	chitosan	734:741	arg1	concentration					717:729	the concentration	713:729	the concentration of chitosan and germination time	713:762	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	2	21	theme	potential	245:253	arg1	applications					255:266	the potential applications	241:266	the potential applications of chitosan	241:278	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	21	theme	potential	245:253	arg1	regulator					300:308	a natural growth regulator	283:308	a natural growth regulator of soybean sprouts	283:327	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	1	22	theme	many	145:148	arg1	countries					156:164	many Asian countries	145:164	many Asian countries	145:164	BACKGROUND Soybeans are popularly known as a healthy food in many Asian countries and are mostly consumed as sprouts.					
30187475	2	23	theme	solutions	486:494	arg1	concentrations					459:472	different concentrations	449:472	different concentrations of chitosan solutions	449:494	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	1	24	theme	Asian	150:154	arg1	countries					156:164	many Asian countries	145:164	many Asian countries	145:164	BACKGROUND Soybeans are popularly known as a healthy food in many Asian countries and are mostly consumed as sprouts.					
30187475	0	25	theme	yellow	60:65	arg1	sprouts					75:81	yellow soybean sprouts	60:81	yellow soybean sprouts	60:81	Effect of chitosan pre-soaking on the growth and quality of yellow soybean sprouts.					
30187475	2	26	theme	natural	285:291	arg1	applications					255:266	the potential applications	241:266	the potential applications of chitosan	241:278	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	26	theme	natural	285:291	arg1	regulator					300:308	a natural growth regulator	283:308	a natural growth regulator of soybean sprouts	283:327	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	6	27	theme	CONCLUSION	1026:1035	arg1	pre-soaking					1046:1056	CONCLUSION Chitosan pre-soaking	1026:1056	CONCLUSION Chitosan pre-soaking	1026:1056	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	2	28	theme	anti-nutrients	401:414	arg1	composition					372:382	composition	372:382	composition	372:382	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	28	theme	anti-nutrients	401:414	arg1	variation					358:366	variation	358:366	variation	358:366	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	6	29	theme	phytic	1209:1214	arg1	acid					1216:1219	phytic acid	1209:1219	phytic acid	1209:1219	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	3	30	theme	soybean	550:556	arg1	sprouts					558:564	the soybean sprouts	546:564	the soybean sprouts	546:564	RESULTS The hypocotyl length and fresh weight of the soybean sprouts could be positively affected by chitosan treatment.					
30187475	4	31	from	changes	630:636	arg1	sprouts					687:693	chitosan-soaked soybean sprouts	663:693	chitosan-soaked soybean sprouts	663:693	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	4	32	theme	products	651:658	arg1	changes					630:636	The content changes	618:636	The content changes of bioactive products in chitosan-soaked soybean sprouts	618:693	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	4	32	theme	products	651:658	arg1	dependent					700:708	dependent	700:708	dependent	700:708	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	0	33	theme	pre-soaking	19:29	arg1	Effect					0:5	Effect	0:5	Effect of chitosan pre-soaking on the growth and quality of yellow soybean sprouts.	0:82	Effect of chitosan pre-soaking on the growth and quality of yellow soybean sprouts.					
30187475	7	34	theme	functional	1383:1392	arg1	foods					1394:1398	low-cost functional foods	1374:1398	low-cost functional foods	1374:1398	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	5	35	located	observed	814:821	arg1	sprouts					860:866	8 g kg-1 chitosan-treated soybean sprouts	826:866	8 g kg-1 chitosan-treated soybean sprouts	826:866	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	5	35	located	observed	814:821	arg2	content					802:808	the lowest phytic acid content	779:808	the lowest phytic acid content	779:808	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	1	36	theme	BACKGROUND	84:93	arg1	sprouts					193:199	sprouts	193:199	sprouts	193:199	BACKGROUND Soybeans are popularly known as a healthy food in many Asian countries and are mostly consumed as sprouts.					
30187475	1	36	theme	BACKGROUND	84:93	arg1	food					137:140	a healthy food	127:140	a healthy food in many Asian countries	127:164	BACKGROUND Soybeans are popularly known as a healthy food in many Asian countries and are mostly consumed as sprouts.					
30187475	1	36	theme	BACKGROUND	84:93	arg1	Soybeans					95:102	BACKGROUND Soybeans	84:102	BACKGROUND Soybeans	84:102	BACKGROUND Soybeans are popularly known as a healthy food in many Asian countries and are mostly consumed as sprouts.					
30187475	6	37	dep	increase	1062:1069	arg1	effect					1148:1153	effect	1148:1153	effect the content of bioactive products in sprouts	1148:1198	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	6	37	dep	increase	1062:1069	arg1	whereas					1201:1207	whereas	1201:1207	whereas	1201:1207	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	2	38	theme	nutrients	387:395	arg1	composition					372:382	composition	372:382	composition	372:382	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	38	theme	nutrients	387:395	arg1	variation					358:366	variation	358:366	variation	358:366	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	3	39	theme	hypocotyl	509:517	arg1	length					519:524	hypocotyl length	509:524	hypocotyl length	509:524	RESULTS The hypocotyl length and fresh weight of the soybean sprouts could be positively affected by chitosan treatment.					
30187475	2	40	theme	chitosan	271:278	arg1	applications					255:266	the potential applications	241:266	the potential applications of chitosan	241:278	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	40	theme	chitosan	271:278	arg1	regulator					300:308	a natural growth regulator	283:308	a natural growth regulator of soybean sprouts	283:327	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	7	41	theme	low-cost	1374:1381	arg1	foods					1394:1398	low-cost functional foods	1374:1398	low-cost functional foods	1374:1398	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	6	42	theme	hypocotyl	1075:1083	arg1	length					1085:1090	hypocotyl length	1075:1090	hypocotyl length	1075:1090	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	6	43	theme	quality	1293:1299	arg1	enhancement					1270:1280	the enhancement	1266:1280	the enhancement of product quality	1266:1299	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	6	44	theme	product	1285:1291	arg1	quality					1293:1299	product quality	1285:1299	product quality	1285:1299	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	6	45	theme	fresh	1096:1100	arg1	weight					1102:1107	fresh weight	1096:1107	fresh weight	1096:1107	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	2	46	theme	present	206:212	arg1	study					214:218	The present study	202:218	The present study	202:218	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	4	47	theme	bioactive	641:649	arg1	products					651:658	bioactive products	641:658	bioactive products	641:658	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	5	48	theme	lowest	783:788	arg1	content					802:808	the lowest phytic acid content	779:808	the lowest phytic acid content	779:808	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	2	49	dep	variation	358:366	arg1	the					354:356	the	354:356	the	354:356	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	5	50	dep	1.56	936:939	arg1	kg-1					943:946	g kg-1	941:946	1.56 g kg-1	936:946	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	4	51	theme	content	622:628	arg1	changes					630:636	The content changes	618:636	The content changes of bioactive products in chitosan-soaked soybean sprouts	618:693	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	4	51	theme	content	622:628	arg1	dependent					700:708	dependent	700:708	dependent	700:708	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	2	52	theme	sprouts	427:433	arg1	anti-nutrients					401:414	anti-nutrients	401:414	anti-nutrients	401:414	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	52	theme	sprouts	427:433	arg1	nutrients					387:395	nutrients	387:395	nutrients	387:395	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	5	53	theme	acid	797:800	arg1	content					802:808	the lowest phytic acid content	779:808	the lowest phytic acid content	779:808	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	7	54	theme	chitosan	1419:1426	arg1	dose					1411:1414	the dose	1407:1414	the dose of chitosan	1407:1426	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	7	54	theme	chitosan	1419:1426	arg1	factor					1437:1442	a key factor	1431:1442	a key factor	1431:1442	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	3	55	theme	sprouts	558:564	arg1	length					519:524	hypocotyl length	509:524	hypocotyl length	509:524	RESULTS The hypocotyl length and fresh weight of the soybean sprouts could be positively affected by chitosan treatment.					
30187475	3	55	theme	sprouts	558:564	arg1	weight					536:541	fresh weight	530:541	fresh weight	530:541	RESULTS The hypocotyl length and fresh weight of the soybean sprouts could be positively affected by chitosan treatment.					
30187475	5	56	dep	content	889:895	arg1	values					897:902	values	897:902	the phytic acid content values after 5 days of germination	873:930	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	2	57	theme	soybean	313:319	arg1	sprouts					321:327	soybean sprouts	313:327	soybean sprouts	313:327	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	58	theme	soybean	419:425	arg1	sprouts					427:433	soybean sprouts	419:433	soybean sprouts pre-soaked in different concentrations of chitosan solutions	419:494	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	6	59	theme	soybean	1112:1118	arg1	sprouts					1120:1126	soybean sprouts	1112:1126	soybean sprouts	1112:1126	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	3	60	dep	length	519:524	arg1	The					505:507	The	505:507	The	505:507	RESULTS The hypocotyl length and fresh weight of the soybean sprouts could be positively affected by chitosan treatment.					
30187475	7	61	theme	key	1433:1435	arg1	dose					1411:1414	the dose	1407:1414	the dose of chitosan	1407:1426	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	7	61	theme	key	1433:1435	arg1	factor					1437:1442	a key factor	1431:1442	a key factor	1431:1442	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	7	62	theme	pre-soaking	1311:1321	arg1	technology					1341:1350	an alternative technology	1326:1350	an alternative technology	1326:1350	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	7	62	theme	pre-soaking	1311:1321	arg1	Chitosan					1302:1309	Chitosan pre-soaking	1302:1321	Chitosan pre-soaking	1302:1321	Chitosan pre-soaking is an alternative technology for the production of low-cost functional foods, where the dose of chitosan is a key factor.					
30187475	6	63	theme	products	1180:1187	arg1	content					1159:1165	the content	1155:1165	the content of bioactive products in sprouts	1155:1198	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	3	64	theme	chitosan	598:605	arg1	treatment					607:615	chitosan treatment	598:615	chitosan treatment	598:615	RESULTS The hypocotyl length and fresh weight of the soybean sprouts could be positively affected by chitosan treatment.					
30187475	4	65	theme	chitosan-soaked	663:677	arg1	sprouts					687:693	chitosan-soaked soybean sprouts	663:693	chitosan-soaked soybean sprouts	663:693	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	4	66	dep	chitosan	734:741	arg1	time					759:762	time	759:762	time	759:762	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	3	67	dep	RESULTS	497:503	arg1	affected					586:593	affected	586:593	could be positively affected by chitosan treatment	566:615	RESULTS The hypocotyl length and fresh weight of the soybean sprouts could be positively affected by chitosan treatment.					
30187475	5	68	theme	ungerminated	1004:1015	arg1	soybean					1017:1023	ungerminated soybean	1004:1023	ungerminated soybean	1004:1023	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	6	69	theme	bioactive	1170:1178	arg1	products					1180:1187	bioactive products	1170:1187	bioactive products	1170:1187	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	2	70	theme	growth	293:298	arg1	applications					255:266	the potential applications	241:266	the potential applications of chitosan	241:278	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	70	theme	growth	293:298	arg1	regulator					300:308	a natural growth regulator	283:308	a natural growth regulator of soybean sprouts	283:327	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	2	71	theme	chitosan	477:484	arg1	solutions					486:494	chitosan solutions	477:494	chitosan solutions	477:494	The present study aimed to investigate the potential applications of chitosan as a natural growth regulator of soybean sprouts, as well as to determine the variation and composition of nutrients and anti-nutrients of soybean sprouts pre-soaked in different concentrations of chitosan solutions.					
30187475	5	72	theme	chitosan-treated	835:850	arg1	sprouts					860:866	8 g kg-1 chitosan-treated soybean sprouts	826:866	8 g kg-1 chitosan-treated soybean sprouts	826:866	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	4	73	theme	soybean	679:685	arg1	sprouts					687:693	chitosan-soaked soybean sprouts	663:693	chitosan-soaked soybean sprouts	663:693	The content changes of bioactive products in chitosan-soaked soybean sprouts were dependent on the concentration of chitosan and germination time.					
30187475	6	74	theme	Chitosan	1037:1044	arg1	pre-soaking					1046:1056	CONCLUSION Chitosan pre-soaking	1026:1056	CONCLUSION Chitosan pre-soaking	1026:1056	CONCLUSION Chitosan pre-soaking can increase the hypocotyl length and fresh weight of soybean sprouts and also positively effect the content of bioactive products in sprouts, whereas phytic acid can be partially removed, thereby leading to the enhancement of product quality.					
30187475	5	75	theme	phytic	790:795	arg1	content					802:808	the lowest phytic acid content	779:808	the lowest phytic acid content	779:808	Additionally, the lowest phytic acid content was observed in 8 g kg-1 chitosan-treated soybean sprouts, and the phytic acid content values after 5 days of germination was 1.56 g kg-1 , which was decreased by 79.0% compared to the value in ungerminated soybean.					
30187475	0	76	theme	chitosan	10:17	arg1	pre-soaking					19:29	chitosan pre-soaking	10:29	chitosan pre-soaking	10:29	Effect of chitosan pre-soaking on the growth and quality of yellow soybean sprouts.					
30187475	1	77	theme	healthy	129:135	arg1	food					137:140	a healthy food	127:140	a healthy food in many Asian countries	127:164	BACKGROUND Soybeans are popularly known as a healthy food in many Asian countries and are mostly consumed as sprouts.					
30187475	1	77	theme	healthy	129:135	arg1	Soybeans					95:102	BACKGROUND Soybeans	84:102	BACKGROUND Soybeans	84:102	BACKGROUND Soybeans are popularly known as a healthy food in many Asian countries and are mostly consumed as sprouts.					
30179152	3	0	theme	Major	346:350	arg1	products					365:372	Major fermentation products	346:372	Major fermentation products of this asaccharolytic organism	346:404	Major fermentation products of this asaccharolytic organism were acetate and butyrate when grown on a complex medium.					
30179152	10	1	theme	related	1099:1105	arg1	caecimuris					1065:1074	Frisingicoccus caecimuris	1050:1074	Frisingicoccus caecimuris	1050:1074	However, sequence identity to other Lachnospiraceae species with validly published names is approximately 93.0 % with Frisingicoccus caecimuris being the most closely related species according to phylogenetic analysis.					
30179152	10	1	theme	related	1099:1105	arg1	species					1107:1113	the most closely related species	1082:1113	the most closely related species according to phylogenetic analysis	1082:1148	However, sequence identity to other Lachnospiraceae species with validly published names is approximately 93.0 % with Frisingicoccus caecimuris being the most closely related species according to phylogenetic analysis.					
30179152	1	2	gly	C-deglycosylating	47:63	arg0	representative					82:95	a C-deglycosylating human intestinal representative	45:95	a C-deglycosylating human intestinal representative of the Lachnospiraceae	45:118	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	1	2	gly	C-deglycosylating	47:63	arg0	nov.					39:42	nov.	39:42	nov.	39:42	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	11	3	theme	type	1287:1290	arg1	CG19-1T					1299:1305	the type strain CG19-1T	1283:1305	the type strain CG19-1T (=DSM 106146T=CCUG 71490T)	1283:1332	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	11	3	theme	type	1287:1290	arg1	71490T					1326:1331	=DSM 106146T=CCUG 71490T	1308:1331	=DSM 106146T=CCUG 71490T	1308:1331	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	7	4	theme	major	745:749	arg1	C16 					777:780	C16 	777:780	C16 	777:780	The major cellular fatty acids were C16 : 0 and C18 : 1ω9c.					
30179152	7	4	theme	major	745:749	arg1	acids					766:770	The major cellular fatty acids	741:770	The major cellular fatty acids	741:770	The major cellular fatty acids were C16 : 0 and C18 : 1ω9c.					
30179152	7	5	theme	cellular	751:758	arg1	C16 					777:780	C16 	777:780	C16 	777:780	The major cellular fatty acids were C16 : 0 and C18 : 1ω9c.					
30179152	7	5	theme	cellular	751:758	arg1	acids					766:770	The major cellular fatty acids	741:770	The major cellular fatty acids	741:770	The major cellular fatty acids were C16 : 0 and C18 : 1ω9c.					
30179152	3	6	theme	organism	397:404	arg1	products					365:372	Major fermentation products	346:372	Major fermentation products of this asaccharolytic organism	346:404	Major fermentation products of this asaccharolytic organism were acetate and butyrate when grown on a complex medium.					
30179152	11	7	theme	strain	1292:1297	arg1	CG19-1T					1299:1305	the type strain CG19-1T	1283:1305	the type strain CG19-1T (=DSM 106146T=CCUG 71490T)	1283:1332	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	11	7	theme	strain	1292:1297	arg1	71490T					1326:1331	=DSM 106146T=CCUG 71490T	1308:1331	=DSM 106146T=CCUG 71490T	1308:1331	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	8	8	theme	47.1 mol	833:840	arg1	G+C content					817:827	The genomic DNA G+C content	801:827	The genomic DNA G+C content	801:827	The genomic DNA G+C content was 47.1 mol%.					
30179152	8	8	theme	47.1 mol	833:840	arg1	%					841:841	47.1 mol%	833:841	47.1 mol%	833:841	The genomic DNA G+C content was 47.1 mol%.					
30179152	5	9	located	observed	550:557	arg1	presence					566:573	the presence	562:573	the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid	562:677	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	5	9	located	observed	550:557	arg2	inhibition					535:544	Growth inhibition	528:544	Growth inhibition	528:544	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	11	10	theme	=DSM	1308:1311	arg1	CG19-1T					1299:1305	the type strain CG19-1T	1283:1305	the type strain CG19-1T (=DSM 106146T=CCUG 71490T)	1283:1332	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	11	10	theme	=DSM	1308:1311	arg1	71490T					1326:1331	=DSM 106146T=CCUG 71490T	1308:1331	=DSM 106146T=CCUG 71490T	1308:1331	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	10	11	with	identity	950:957	arg1	names					1015:1019	validly published names	997:1019	validly published names	997:1019	However, sequence identity to other Lachnospiraceae species with validly published names is approximately 93.0 % with Frisingicoccus caecimuris being the most closely related species according to phylogenetic analysis.					
30179152	2	12	theme	non-spore-forming	155:171	arg1	bacterium					188:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium	121:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides	121:287	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	1	13	theme	C-deglycosylating	47:63	arg1	representative					82:95	a C-deglycosylating human intestinal representative	45:95	a C-deglycosylating human intestinal representative of the Lachnospiraceae	45:118	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	1	13	theme	C-deglycosylating	47:63	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	5	14	theme	Growth	528:533	arg1	inhibition					535:544	Growth inhibition	528:544	Growth inhibition	528:544	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	11	15	with	species	1244:1250	arg1	CG19-1T					1299:1305	the type strain CG19-1T	1283:1305	the type strain CG19-1T (=DSM 106146T=CCUG 71490T)	1283:1332	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	11	15	with	species	1244:1250	arg1	71490T					1326:1331	=DSM 106146T=CCUG 71490T	1308:1331	=DSM 106146T=CCUG 71490T	1308:1331	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	3	16	theme	asaccharolytic	382:395	arg1	organism					397:404	this asaccharolytic organism	377:404	this asaccharolytic organism	377:404	Major fermentation products of this asaccharolytic organism were acetate and butyrate when grown on a complex medium.					
30179152	5	17	theme	dihydroferulic	659:672	arg1	acid					674:677	dihydroferulic acid	659:677	dihydroferulic acid	659:677	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	1	18	theme	human	65:69	arg1	representative					82:95	a C-deglycosylating human intestinal representative	45:95	a C-deglycosylating human intestinal representative of the Lachnospiraceae	45:118	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	1	18	theme	human	65:69	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	2	19	theme	Gram-stain-positive	134:152	arg1	bacterium					188:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium	121:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides	121:287	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	9	20	theme	rRNA	861:864	arg1	sequence					871:878	its 16S rRNA gene sequence	853:878	its 16S rRNA gene sequence	853:878	Based on its 16S rRNA gene sequence, strain CG19-1T is a member of the Lachnospiraceae.					
30179152	5	21	theme	ferulic	611:617	arg1	acid					619:622	ferulic acid	611:622	ferulic acid	611:622	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	1	22	theme	intestinal	71:80	arg1	representative					82:95	a C-deglycosylating human intestinal representative	45:95	a C-deglycosylating human intestinal representative of the Lachnospiraceae	45:118	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	1	22	theme	intestinal	71:80	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	10	23	theme	phylogenetic	1128:1139	arg1	analysis					1141:1148	phylogenetic analysis	1128:1148	phylogenetic analysis	1128:1148	However, sequence identity to other Lachnospiraceae species with validly published names is approximately 93.0 % with Frisingicoccus caecimuris being the most closely related species according to phylogenetic analysis.					
30179152	9	24	theme	strain	881:886	arg1	CG19-1T					888:894	strain CG19-1T	881:894	strain CG19-1T	881:894	Based on its 16S rRNA gene sequence, strain CG19-1T is a member of the Lachnospiraceae.					
30179152	9	24	theme	strain	881:886	arg1	member					901:906	a member	899:906	a member of the Lachnospiraceae	899:929	Based on its 16S rRNA gene sequence, strain CG19-1T is a member of the Lachnospiraceae.					
30179152	9	25	theme	gene	866:869	arg1	sequence					871:878	its 16S rRNA gene sequence	853:878	its 16S rRNA gene sequence	853:878	Based on its 16S rRNA gene sequence, strain CG19-1T is a member of the Lachnospiraceae.					
30179152	7	26	theme	fatty	760:764	arg1	C16 					777:780	C16 	777:780	C16 	777:780	The major cellular fatty acids were C16 : 0 and C18 : 1ω9c.					
30179152	7	26	theme	fatty	760:764	arg1	acids					766:770	The major cellular fatty acids	741:770	The major cellular fatty acids	741:770	The major cellular fatty acids were C16 : 0 and C18 : 1ω9c.					
30179152	11	27	theme	106146T=CCUG	1313:1324	arg1	CG19-1T					1299:1305	the type strain CG19-1T	1283:1305	the type strain CG19-1T (=DSM 106146T=CCUG 71490T)	1283:1332	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	11	27	theme	106146T=CCUG	1313:1324	arg1	71490T					1326:1331	=DSM 106146T=CCUG 71490T	1308:1331	=DSM 106146T=CCUG 71490T	1308:1331	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	10	28	theme	published	1005:1013	arg1	names					1015:1019	validly published names	997:1019	validly published names	997:1019	However, sequence identity to other Lachnospiraceae species with validly published names is approximately 93.0 % with Frisingicoccus caecimuris being the most closely related species according to phylogenetic analysis.					
30179152	10	29	theme	other	962:966	arg1	species					984:990	other Lachnospiraceae species	962:990	other Lachnospiraceae species	962:990	However, sequence identity to other Lachnospiraceae species with validly published names is approximately 93.0 % with Frisingicoccus caecimuris being the most closely related species according to phylogenetic analysis.					
30179152	2	30	theme	taxonomic	326:334	arg1	approach					336:343	a polyphasic taxonomic approach	313:343	a polyphasic taxonomic approach	313:343	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	2	31	attach	isolated	198:205	arg1	gut					222:224	the human gut	212:224	the human gut	212:224	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	2	31	attach	isolated	198:205	arg2	bacterium					188:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium	121:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides	121:287	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	0	32	theme	gen.	24:27	arg1	sp					35:36	sp	35:36	sp	35:36	Catenibacillus scindens gen. nov., sp.					
30179152	0	32	theme	gen.	24:27	arg1	nov.					29:32	gen. nov.	24:32	gen. nov.	24:32	Catenibacillus scindens gen. nov., sp.					
30179152	11	33	theme	novel	1238:1242	arg1	species					1244:1250	a novel species	1236:1250	a novel species	1236:1250	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	11	33	theme	novel	1238:1242	arg1	scindens					1268:1275	Catenibacillus scindens	1253:1275	Catenibacillus scindens	1253:1275	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	4	34	theme	CG19-1T	481:487	arg1	Growth					464:469	Growth	464:469	Growth of strain CG19-1T	464:487	Growth of strain CG19-1T was stimulated by glucose or pyruvate.					
30179152	2	35	theme	polyphasic	315:324	arg1	approach					336:343	a polyphasic taxonomic approach	313:343	a polyphasic taxonomic approach	313:343	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	6	36	theme	A4β	724:726	arg1	l-Orn-d-Asp					728:738	peptidoglycan type A4β l-Orn-d-Asp	705:738	peptidoglycan type A4β l-Orn-d-Asp	705:738	Strain CG19-1T contained peptidoglycan type A4β l-Orn-d-Asp.					
30179152	6	37	contain	contained	695:703	arg1	CG19-1T					687:693	Strain CG19-1T	680:693	Strain CG19-1T	680:693	Strain CG19-1T contained peptidoglycan type A4β l-Orn-d-Asp.					
30179152	6	37	contain	contained	695:703	arg2	l-Orn-d-Asp					728:738	peptidoglycan type A4β l-Orn-d-Asp	705:738	peptidoglycan type A4β l-Orn-d-Asp	705:738	Strain CG19-1T contained peptidoglycan type A4β l-Orn-d-Asp.					
30179152	4	38	theme	strain	474:479	arg1	CG19-1T					481:487	strain CG19-1T	474:487	strain CG19-1T	474:487	Growth of strain CG19-1T was stimulated by glucose or pyruvate.					
30179152	2	39	theme	capable	247:253	arg1	gut					222:224	the human gut	212:224	the human gut	212:224	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	6	40	theme	type	719:722	arg1	l-Orn-d-Asp					728:738	peptidoglycan type A4β l-Orn-d-Asp	705:738	peptidoglycan type A4β l-Orn-d-Asp	705:738	Strain CG19-1T contained peptidoglycan type A4β l-Orn-d-Asp.					
30179152	3	41	theme	complex	448:454	arg1	medium					456:461	a complex medium	446:461	a complex medium	446:461	Major fermentation products of this asaccharolytic organism were acetate and butyrate when grown on a complex medium.					
30179152	11	42	with	genus	1209:1213	arg1	CG19-1T					1299:1305	the type strain CG19-1T	1283:1305	the type strain CG19-1T (=DSM 106146T=CCUG 71490T)	1283:1332	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	11	42	with	genus	1209:1213	arg1	71490T					1326:1331	=DSM 106146T=CCUG 71490T	1308:1331	=DSM 106146T=CCUG 71490T	1308:1331	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	8	43	theme	DNA	813:815	arg1	G+C content					817:827	The genomic DNA G+C content	801:827	The genomic DNA G+C content	801:827	The genomic DNA G+C content was 47.1 mol%.					
30179152	8	43	theme	DNA	813:815	arg1	%					841:841	47.1 mol%	833:841	47.1 mol%	833:841	The genomic DNA G+C content was 47.1 mol%.					
30179152	2	44	theme	non-motile	177:186	arg1	bacterium					188:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium	121:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides	121:287	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	10	45	theme	Lachnospiraceae	968:982	arg1	species					984:990	other Lachnospiraceae species	962:990	other Lachnospiraceae species	962:990	However, sequence identity to other Lachnospiraceae species with validly published names is approximately 93.0 % with Frisingicoccus caecimuris being the most closely related species according to phylogenetic analysis.					
30179152	5	46	theme	several	578:584	arg1	acid					619:622	ferulic acid	611:622	ferulic acid	611:622	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	5	46	theme	several	578:584	arg1	acids					595:599	several phenolic acids	578:599	several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid	578:677	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	1	47	theme	Lachnospiraceae	104:118	arg1	representative					82:95	a C-deglycosylating human intestinal representative	45:95	a C-deglycosylating human intestinal representative of the Lachnospiraceae	45:118	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	1	47	theme	Lachnospiraceae	104:118	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov., a C-deglycosylating human intestinal representative of the Lachnospiraceae.					
30179152	9	48	theme	16S	857:859	arg1	sequence					871:878	its 16S rRNA gene sequence	853:878	its 16S rRNA gene sequence	853:878	Based on its 16S rRNA gene sequence, strain CG19-1T is a member of the Lachnospiraceae.					
30179152	5	49	theme	phenolic	586:593	arg1	acid					619:622	ferulic acid	611:622	ferulic acid	611:622	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	5	49	theme	phenolic	586:593	arg1	acids					595:599	several phenolic acids	578:599	several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid	578:677	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	6	50	theme	peptidoglycan	705:717	arg1	l-Orn-d-Asp					728:738	peptidoglycan type A4β l-Orn-d-Asp	705:738	peptidoglycan type A4β l-Orn-d-Asp	705:738	Strain CG19-1T contained peptidoglycan type A4β l-Orn-d-Asp.					
30179152	5	51	theme	acids	595:599	arg1	presence					566:573	the presence	562:573	the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid	562:677	Growth inhibition was observed in the presence of several phenolic acids including ferulic acid, which nevertheless was reduced to dihydroferulic acid.					
30179152	10	52	theme	sequence	941:948	arg1	identity					950:957	sequence identity	941:957	sequence identity to other Lachnospiraceae species with validly published names	941:1019	However, sequence identity to other Lachnospiraceae species with validly published names is approximately 93.0 % with Frisingicoccus caecimuris being the most closely related species according to phylogenetic analysis.					
30179152	2	53	theme	human	216:220	arg1	gut					222:224	the human gut	212:224	the human gut	212:224	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	2	54	theme	aromatic	267:274	arg1	C-glucosides					276:287	aromatic C-glucosides	267:287	aromatic C-glucosides	267:287	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	3	55	theme	fermentation	352:363	arg1	products					365:372	Major fermentation products	346:372	Major fermentation products of this asaccharolytic organism	346:404	Major fermentation products of this asaccharolytic organism were acetate and butyrate when grown on a complex medium.					
30179152	6	56	theme	Strain	680:685	arg1	CG19-1T					687:693	Strain CG19-1T	680:693	Strain CG19-1T	680:693	Strain CG19-1T contained peptidoglycan type A4β l-Orn-d-Asp.					
30179152	8	57	theme	genomic	805:811	arg1	G+C content					817:827	The genomic DNA G+C content	801:827	The genomic DNA G+C content	801:827	The genomic DNA G+C content was 47.1 mol%.					
30179152	8	57	theme	genomic	805:811	arg1	%					841:841	47.1 mol%	833:841	47.1 mol%	833:841	The genomic DNA G+C content was 47.1 mol%.					
30179152	9	58	theme	Lachnospiraceae	915:929	arg1	CG19-1T					888:894	strain CG19-1T	881:894	strain CG19-1T	881:894	Based on its 16S rRNA gene sequence, strain CG19-1T is a member of the Lachnospiraceae.					
30179152	9	58	theme	Lachnospiraceae	915:929	arg1	member					901:906	a member	899:906	a member of the Lachnospiraceae	899:929	Based on its 16S rRNA gene sequence, strain CG19-1T is a member of the Lachnospiraceae.					
30179152	2	59	theme	anaerobic	124:132	arg1	bacterium					188:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium	121:196	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides	121:287	An anaerobic Gram-stain-positive, non-spore-forming and non-motile bacterium isolated from the human gut, designated CG19-1T, capable of cleaving aromatic C-glucosides was characterized using a polyphasic taxonomic approach.					
30179152	11	60	with	Catenibacillus	1216:1229	arg1	CG19-1T					1299:1305	the type strain CG19-1T	1283:1305	the type strain CG19-1T (=DSM 106146T=CCUG 71490T)	1283:1332	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	11	60	with	Catenibacillus	1216:1229	arg1	71490T					1326:1331	=DSM 106146T=CCUG 71490T	1308:1331	=DSM 106146T=CCUG 71490T	1308:1331	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30179152	11	61	theme	novel	1203:1207	arg1	genus					1209:1213	a novel genus	1201:1213	a novel genus	1201:1213	Based on these findings, it is proposed to create a novel genus, Catenibacillus, and a novel species, Catenibacillus scindens, with the type strain CG19-1T (=DSM 106146T=CCUG 71490T).					
30530944	4	0	theme	strain	937:942	arg1	levels					944:949	strain levels	937:949	strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%)	937:1049	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	0	1	with	construct	26:34	arg1	composition					67:77	zone-specific biochemical composition	41:77	zone-specific biochemical composition	41:77	A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus.					
30530944	6	2	theme	type	1332:1335	arg1	ratio					1314:1318	ratio	1314:1318	ratio of collagen type I (COL 1)	1314:1345	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	2	3	theme	inner	507:511	arg1	regions					523:529	the inner and outer regions	503:529	the inner and outer regions	503:529	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	6	4	from	%	1278:1278	arg1	region					1256:1261	the Ag-impregnated inner region	1231:1261	the Ag-impregnated inner region (from 50% to 60% of total collagen)	1231:1297	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	4	from	%	1278:1278	arg1	ratio					1194:1198	increased ratio	1184:1198	increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen)	1184:1297	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	5	theme	type	1212:1215	arg1	ratio					1194:1198	increased ratio	1184:1198	increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen)	1184:1297	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	1	6	theme	bizonal	162:168	arg1	structure					170:178	its bizonal structure	158:178	its bizonal structure	158:178	Engineering the meniscus is challenging due to its bizonal structure; the tissue is cartilaginous at the inner portion and fibrous at the outer portion.					
30530944	4	7	theme	initial	1001:1007	arg1	thickness					1009:1017	initial thickness	1001:1017	initial thickness	1001:1017	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	4	8	theme	PCL/hydrogel	882:893	arg1	constructs					895:904	meniscus shaped PCL/hydrogel constructs	866:904	meniscus shaped PCL/hydrogel constructs	866:904	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	6	9	theme	collagen	1203:1210	arg1	type					1212:1215	collagen type II	1203:1218	collagen type II (COL 2)	1203:1226	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	9	theme	collagen	1203:1210	arg1	COL					1221:1223	COL 2	1221:1225	COL 2	1221:1225	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	8	10	theme	meniscus	1596:1603	arg1	replacement					1605:1615	total meniscus replacement	1590:1615	total meniscus replacement	1590:1615	Our construct has a potential for use as a substitute for total meniscus replacement.					
30530944	6	11	theme	%	1393:1393	arg1	%					1400:1400	60% to 70%	1391:1400	60% to 70%	1391:1400	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	1	12	theme	outer	249:253	arg1	portion					255:261	the outer portion	245:261	the outer portion	245:261	Engineering the meniscus is challenging due to its bizonal structure; the tissue is cartilaginous at the inner portion and fibrous at the outer portion.					
30530944	4	13	theme	shaped	875:880	arg1	constructs					895:904	meniscus shaped PCL/hydrogel constructs	866:904	meniscus shaped PCL/hydrogel constructs	866:904	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	4	14	theme	outer	981:985	arg1	region					987:992	the outer region	977:992	the outer region (2% of initial thickness) towards the inner region (10%)	977:1049	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	5	15	theme	dynamic	1136:1142	arg1	stress					1144:1149	dynamic stress	1136:1149	dynamic stress	1136:1149	Incorporation of hydrogels protected the cells from the mechanical damage caused by dynamic stress.					
30530944	7	16	from	portion	1523:1529	arg1	fibrocartilage-like					1490:1508	fibrocartilage-like	1490:1508	fibrocartilage-like	1490:1508	We were able to engineer a meniscus, which is cartilage-like at the inner portion and fibrocartilage-like at the outer portion.					
30530944	3	17	theme	collagen	759:766	arg1	production					768:777	collagen production	759:777	collagen production	759:777	After incubating the constructs loaded with porcine fibrochondrocytes for 8 weeks, we demonstrated that presence of Ag enhanced glycosaminoglycan (GAG) production by about 4 fold (p < 0.001), while GelMA enhanced collagen production by about 50 fold (p < 0.001).					
30530944	3	18	theme	glycosaminoglycan	674:690	arg1	production					698:707	glycosaminoglycan (GAG) production	674:707	glycosaminoglycan (GAG) production	674:707	After incubating the constructs loaded with porcine fibrochondrocytes for 8 weeks, we demonstrated that presence of Ag enhanced glycosaminoglycan (GAG) production by about 4 fold (p < 0.001), while GelMA enhanced collagen production by about 50 fold (p < 0.001).					
30530944	4	19	dep	region	987:992	arg1	%					996:996	2%	995:996	2% of initial thickness	995:1017	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	4	19	dep	region	987:992	arg1	thickness					1009:1017	initial thickness	1001:1017	initial thickness	1001:1017	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	0	20	theme	3D	2:3	arg1	construct					26:34	A 3D printed PCL/hydrogel construct	0:34	A 3D printed PCL/hydrogel construct with zone-specific biochemical composition	0:77	A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus.					
30530944	6	21	theme	%	1271:1271	arg1	collagen					1289:1296	total collagen	1283:1296	total collagen	1283:1296	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	21	theme	%	1271:1271	arg1	%					1278:1278	50% to 60%	1269:1278	50% to 60% of total collagen	1269:1296	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	22	dep	%	1278:1278	arg1	to					1273:1274	to	1273:1274	to	1273:1274	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	2	23	theme	3D	371:372	arg1	printing					374:381	3D printing	371:381	3D printing	371:381	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	2	24	theme	artificial	288:297	arg1	meniscus					299:306	an artificial meniscus	285:306	an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold	285:412	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	6	25	theme	Ag-impregnated	1235:1248	arg1	region					1256:1261	the Ag-impregnated inner region	1231:1261	the Ag-impregnated inner region (from 50% to 60% of total collagen)	1231:1297	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	26	dep	increased	1304:1312	arg1	%					1400:1400	60% to 70%	1391:1400	60% to 70%	1391:1400	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	0	27	theme	PCL/hydrogel	13:24	arg1	construct					26:34	A 3D printed PCL/hydrogel construct	0:34	A 3D printed PCL/hydrogel construct with zone-specific biochemical composition	0:77	A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus.					
30530944	7	28	theme	inner	1472:1476	arg1	portion					1478:1484	the inner portion	1468:1484	the inner portion	1468:1484	We were able to engineer a meniscus, which is cartilage-like at the inner portion and fibrocartilage-like at the outer portion.					
30530944	6	29	theme	inner	1250:1254	arg1	region					1256:1261	the Ag-impregnated inner region	1231:1261	the Ag-impregnated inner region (from 50% to 60% of total collagen)	1231:1297	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	2	30	theme	outer	517:521	arg1	regions					523:529	the inner and outer regions	503:529	the inner and outer regions	503:529	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	4	31	theme	inner	1032:1036	arg1	region					1038:1043	the inner region	1028:1043	the inner region (10%)	1028:1049	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	4	31	theme	inner	1032:1036	arg1	%					1048:1048	10%	1046:1048	10%	1046:1048	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	0	32	theme	printed	5:11	arg1	construct					26:34	A 3D printed PCL/hydrogel construct	0:34	A 3D printed PCL/hydrogel construct with zone-specific biochemical composition	0:77	A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus.					
30530944	1	33	from	portion	255:261	arg1	fibrous					234:240	fibrous	234:240	fibrous	234:240	Engineering the meniscus is challenging due to its bizonal structure; the tissue is cartilaginous at the inner portion and fibrous at the outer portion.					
30530944	2	34	theme	PCL	401:403	arg1	scaffold					405:412	a meniscus shaped PCL scaffold	383:412	an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold	285:412	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	6	35	from	ratio	1194:1198	arg1	collagen					1289:1296	total collagen	1283:1296	total collagen	1283:1296	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	35	from	ratio	1194:1198	arg1	region					1256:1261	the Ag-impregnated inner region	1231:1261	the Ag-impregnated inner region (from 50% to 60% of total collagen)	1231:1297	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	35	from	ratio	1194:1198	arg1	%					1278:1278	50% to 60%	1269:1278	50% to 60% of total collagen	1269:1296	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	5	36	theme	mechanical	1108:1117	arg1	damage					1119:1124	the mechanical damage	1104:1124	the mechanical damage caused by dynamic stress	1104:1149	Incorporation of hydrogels protected the cells from the mechanical damage caused by dynamic stress.					
30530944	4	37	theme	meniscus	866:873	arg1	constructs					895:904	meniscus shaped PCL/hydrogel constructs	866:904	meniscus shaped PCL/hydrogel constructs	866:904	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	6	38	theme	collagen	1323:1330	arg1	type					1332:1335	collagen type I	1323:1337	collagen type I (COL 1)	1323:1345	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	38	theme	collagen	1323:1330	arg1	COL					1340:1342	COL 1	1340:1344	COL 1	1340:1344	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	39	theme	outer	1372:1376	arg1	region					1378:1383	the GelMA-impregnated outer region	1350:1383	the GelMA-impregnated outer region	1350:1383	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	2	40	theme	shaped	394:399	arg1	scaffold					405:412	a meniscus shaped PCL scaffold	383:412	an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold	285:412	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	6	41	theme	collagen	1289:1296	arg1	collagen					1289:1296	total collagen	1283:1296	total collagen	1283:1296	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	41	theme	collagen	1289:1296	arg1	%					1278:1278	50% to 60%	1269:1278	50% to 60% of total collagen	1269:1296	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	42	theme	Dynamic	1152:1158	arg1	stimulation					1160:1170	Dynamic stimulation	1152:1170	Dynamic stimulation	1152:1170	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	2	43	theme	meniscus	385:392	arg1	scaffold					405:412	a meniscus shaped PCL scaffold	383:412	an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold	285:412	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	2	44	theme	agarose	444:450	arg1	hydrogels					490:498	agarose (Ag) and gelatin methacrylate (GelMA) hydrogels	444:498	agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions	444:529	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	5	45	theme	hydrogels	1069:1077	arg1	Incorporation					1052:1064	Incorporation	1052:1064	Incorporation of hydrogels	1052:1077	Incorporation of hydrogels protected the cells from the mechanical damage caused by dynamic stress.					
30530944	2	46	theme	gelatin	461:467	arg1	GelMA					483:487	GelMA	483:487	GelMA	483:487	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	2	46	theme	gelatin	461:467	arg1	methacrylate					469:480	gelatin methacrylate	461:480	gelatin methacrylate (GelMA)	461:488	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	0	47	theme	zone-specific	41:53	arg1	composition					67:77	zone-specific biochemical composition	41:77	zone-specific biochemical composition	41:77	A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus.					
30530944	6	48	theme	total	1283:1287	arg1	collagen					1289:1296	total collagen	1283:1296	total collagen	1283:1296	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	8	49	contain	has	1546:1548	arg1	construct					1536:1544	Our construct	1532:1544	Our construct	1532:1544	Our construct has a potential for use as a substitute for total meniscus replacement.					
30530944	8	49	contain	has	1546:1548	arg2	potential					1552:1560	a potential	1550:1560	a potential for use	1550:1568	Our construct has a potential for use as a substitute for total meniscus replacement.					
30530944	6	50	theme	increased	1184:1192	arg1	ratio					1194:1198	increased ratio	1184:1198	increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen)	1184:1297	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	2	51	theme	biochemical	322:332	arg1	organization					334:345	the biochemical organization	318:345	the biochemical organization of the native tissue	318:366	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	4	52	theme	physiological	831:843	arg1	environment					853:863	the physiological loading environment	827:863	the physiological loading environment	827:863	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	7	53	from	portion	1478:1484	arg1	cartilage-like					1450:1463	cartilage-like	1450:1463	cartilage-like	1450:1463	We were able to engineer a meniscus, which is cartilage-like at the inner portion and fibrocartilage-like at the outer portion.					
30530944	7	53	from	portion	1478:1484	arg1	meniscus					1431:1438	a meniscus	1429:1438	a meniscus	1429:1438	We were able to engineer a meniscus, which is cartilage-like at the inner portion and fibrocartilage-like at the outer portion.					
30530944	2	54	with	impregnating	423:434	arg1	hydrogels					490:498	agarose (Ag) and gelatin methacrylate (GelMA) hydrogels	444:498	agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions	444:529	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	2	55	dep	meniscus	299:306	arg1	scaffold					405:412	a meniscus shaped PCL scaffold	383:412	an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold	285:412	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	4	56	theme	thickness	1009:1017	arg1	%					996:996	2%	995:996	2% of initial thickness	995:1017	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	4	56	theme	thickness	1009:1017	arg1	thickness					1009:1017	initial thickness	1001:1017	initial thickness	1001:1017	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	1	57	theme	inner	216:220	arg1	portion					222:228	the inner portion	212:228	the inner portion	212:228	Engineering the meniscus is challenging due to its bizonal structure; the tissue is cartilaginous at the inner portion and fibrous at the outer portion.					
30530944	1	58	from	portion	222:228	arg1	cartilaginous					195:207	cartilaginous	195:207	cartilaginous	195:207	Engineering the meniscus is challenging due to its bizonal structure; the tissue is cartilaginous at the inner portion and fibrous at the outer portion.					
30530944	1	58	from	portion	222:228	arg1	tissue					185:190	the tissue	181:190	the tissue	181:190	Engineering the meniscus is challenging due to its bizonal structure; the tissue is cartilaginous at the inner portion and fibrous at the outer portion.					
30530944	2	59	from	hydrogels	490:498	arg1	regions					523:529	the inner and outer regions	503:529	the inner and outer regions	503:529	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	3	60	theme	porcine	590:596	arg1	fibrochondrocytes					598:614	porcine fibrochondrocytes	590:614	porcine fibrochondrocytes	590:614	After incubating the constructs loaded with porcine fibrochondrocytes for 8 weeks, we demonstrated that presence of Ag enhanced glycosaminoglycan (GAG) production by about 4 fold (p < 0.001), while GelMA enhanced collagen production by about 50 fold (p < 0.001).					
30530944	6	61	theme	GelMA-impregnated	1354:1370	arg1	region					1378:1383	the GelMA-impregnated outer region	1350:1383	the GelMA-impregnated outer region	1350:1383	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	6	62	dep	%	1400:1400	arg1	to					1395:1396	to	1395:1396	to	1395:1396	Dynamic stimulation resulted in increased ratio of collagen type II (COL 2) in the Ag-impregnated inner region (from 50% to 60% of total collagen), and increased ratio of collagen type I (COL 1) in the GelMA-impregnated outer region (from 60% to 70%).					
30530944	4	63	theme	loading	845:851	arg1	environment					853:863	the physiological loading environment	827:863	the physiological loading environment	827:863	In order to mimic the physiological loading environment, meniscus shaped PCL/hydrogel constructs were dynamically stimulated at strain levels gradually increasing from the outer region (2% of initial thickness) towards the inner region (10%).					
30530944	2	64	theme	methacrylate	469:480	arg1	hydrogels					490:498	agarose (Ag) and gelatin methacrylate (GelMA) hydrogels	444:498	agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions	444:529	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	7	65	theme	outer	1517:1521	arg1	portion					1523:1529	the outer portion	1513:1529	the outer portion	1513:1529	We were able to engineer a meniscus, which is cartilage-like at the inner portion and fibrocartilage-like at the outer portion.					
30530944	2	66	theme	tissue	361:366	arg1	organization					334:345	the biochemical organization	318:345	the biochemical organization of the native tissue	318:366	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30530944	8	67	dep	a	1573:1573	arg1	substitute					1575:1584	substitute	1575:1584	substitute for total meniscus replacement	1575:1615	Our construct has a potential for use as a substitute for total meniscus replacement.					
30530944	0	68	theme	biochemical	55:65	arg1	composition					67:77	zone-specific biochemical composition	41:77	zone-specific biochemical composition	41:77	A 3D printed PCL/hydrogel construct with zone-specific biochemical composition mimicking that of the meniscus.					
30530944	3	69	theme	Ag	662:663	arg1	presence					650:657	presence	650:657	presence of Ag	650:663	After incubating the constructs loaded with porcine fibrochondrocytes for 8 weeks, we demonstrated that presence of Ag enhanced glycosaminoglycan (GAG) production by about 4 fold (p < 0.001), while GelMA enhanced collagen production by about 50 fold (p < 0.001).					
30530944	8	70	theme	total	1590:1594	arg1	replacement					1605:1615	total meniscus replacement	1590:1615	total meniscus replacement	1590:1615	Our construct has a potential for use as a substitute for total meniscus replacement.					
30530944	2	71	theme	native	354:359	arg1	tissue					361:366	the native tissue	350:366	the native tissue	350:366	Here, we constructed an artificial meniscus mimicking the biochemical organization of the native tissue by 3D printing a meniscus shaped PCL scaffold and then impregnating it with agarose (Ag) and gelatin methacrylate (GelMA) hydrogels in the inner and outer regions, respectively.					
30003974	0	0	theme	genus	74:78	arg1	tuber-regium					101:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	0	1	from	genome	12:17	arg1	tuber-regium					101:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	3	2	theme	glucan	706:711	arg1	biosynthesis					721:732	glucan reserve biosynthesis	706:732	glucan reserve biosynthesis	706:732	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	3	3	theme	compound	683:690	arg1	breakdown					692:700	cellulosic compound breakdown	672:700	cellulosic compound breakdown	672:700	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	0	4	theme	conversion	168:177	arg1	mechanism					143:151	the mechanism	139:151	the mechanism of its biomass conversion from lignocellulose substrates	139:208	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	0	5	theme	-Pleurotus	90:99	arg1	tuber-regium					101:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	0	6	theme	Pleurotus	80:88	arg1	tuber-regium					101:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	0	7	theme	lignocellulose	184:197	arg1	substrates					199:208	lignocellulose substrates	184:208	lignocellulose substrates	184:208	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	4	8	theme	new	769:771	arg1	insights					773:780	new insights	769:780	new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus	769:857	The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus.					
30003974	0	9	from	mechanism	143:151	arg1	substrates					199:208	lignocellulose substrates	184:208	lignocellulose substrates	184:208	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	3	10	theme	better	447:452	arg1	understanding					454:466	a better understanding	445:466	a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis	445:732	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	4	11	theme	lignocellulose	786:799	arg1	bioconversion					801:813	lignocellulose bioconversion	786:813	lignocellulose bioconversion of white-rot fungus for the genus Pleurotus	786:857	The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus.					
30003974	3	12	theme	dikaryon	397:404	arg1	strain					427:432	a dikaryon P. tuber-regium wild strain	395:432	a dikaryon P. tuber-regium wild strain	395:432	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	3	13	with	reserves	577:584	arg1	reference					636:644	reference	636:644	reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis	636:732	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	3	14	theme	reserve	713:719	arg1	biosynthesis					721:732	glucan reserve biosynthesis	706:732	glucan reserve biosynthesis	706:732	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	3	15	theme	P.	406:407	arg1	strain					427:432	a dikaryon P. tuber-regium wild strain	395:432	a dikaryon P. tuber-regium wild strain	395:432	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	0	16	theme	diploid	4:10	arg1	genome					12:17	The diploid genome	0:17	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium -	0:114	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	4	17	theme	genomic	747:753	arg1	data					755:758	The present genomic data	735:758	The present genomic data	735:758	The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus.					
30003974	0	18	from	substrates	199:208	arg1	conversion					168:177	its biomass conversion	156:177	its biomass conversion from lignocellulose substrates	156:208	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	0	18	from	substrates	199:208	arg1	mechanism					143:151	the mechanism	139:151	the mechanism of its biomass conversion from lignocellulose substrates	139:208	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	3	19	theme	sclerotia-forming	594:610	arg1	mushroom					622:629	this sclerotia-forming Pleurotus mushroom	589:629	this sclerotia-forming Pleurotus mushroom	589:629	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	3	20	dep	P.	406:407	arg1	tuber-regium					409:420	tuber-regium	409:420	tuber-regium	409:420	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	3	21	theme	strain	427:432	arg1	genome					385:390	the genome	381:390	the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis	381:732	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	4	22	theme	present	739:745	arg1	data					755:758	The present genomic data	735:758	The present genomic data	735:758	The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus.					
30003974	2	23	theme	white-rot	256:264	arg1	fungus					266:271	a white-rot fungus	254:271	a white-rot fungus	254:271	Singer, being a white-rot fungus, is widely used for food and medicine in the Asia-Pacific region.					
30003974	1	24	theme	Pleurotus	211:219	arg1	tuber-regium					221:232	Pleurotus tuber-regium	211:232	Pleurotus tuber-regium (Fr.)	211:238	Pleurotus tuber-regium (Fr.)					
30003974	3	25	theme	beta-glucan	565:575	arg1	reserves					577:584	beta-glucan reserves	565:584	beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis	565:732	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	3	26	from	reserves	577:584	arg1	mushroom					622:629	this sclerotia-forming Pleurotus mushroom	589:629	this sclerotia-forming Pleurotus mushroom	589:629	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	3	27	theme	wild	422:425	arg1	strain					427:432	a dikaryon P. tuber-regium wild strain	395:432	a dikaryon P. tuber-regium wild strain	395:432	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	0	28	theme	wild-type	49:57	arg1	species					59:65	the only sclerotia-forming wild-type species	22:65	the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium -	22:114	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	4	29	theme	genus	843:847	arg1	Pleurotus					849:857	the genus Pleurotus	839:857	the genus Pleurotus	839:857	The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus.					
30003974	0	30	from	species	59:65	arg1	tuber-regium					101:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	the genus Pleurotus -Pleurotus tuber-regium	70:112	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	3	31	theme	lignocellulose	547:560	arg1	bio-conversion					529:542	the bio-conversion	525:542	the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis	525:732	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	2	32	theme	Asia-Pacific	318:329	arg1	region					331:336	the Asia-Pacific region	314:336	the Asia-Pacific region	314:336	Singer, being a white-rot fungus, is widely used for food and medicine in the Asia-Pacific region.					
30003974	0	33	theme	sclerotia-forming	31:47	arg1	species					59:65	the only sclerotia-forming wild-type species	22:65	the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium -	22:114	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	3	34	theme	cellulosic	672:681	arg1	breakdown					692:700	cellulosic compound breakdown	672:700	cellulosic compound breakdown	672:700	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	2	35	used	used	284:287	arg2	Singer					240:245	Singer	240:245	Singer	240:245	Singer, being a white-rot fungus, is widely used for food and medicine in the Asia-Pacific region.					
30003974	0	36	theme	biomass	160:166	arg1	conversion					168:177	its biomass conversion	156:177	its biomass conversion from lignocellulose substrates	156:208	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	0	37	from	tuber-regium	101:112	arg1	genome					12:17	The diploid genome	0:17	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium -	0:114	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	1	38	dep	tuber-regium	221:232	arg1	Fr					235:236	Fr	235:236	Fr.	235:237	Pleurotus tuber-regium (Fr.)					
30003974	3	39	theme	Pleurotus	612:620	arg1	mushroom					622:629	this sclerotia-forming Pleurotus mushroom	589:629	this sclerotia-forming Pleurotus mushroom	589:629	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	4	40	theme	fungus	828:833	arg1	bioconversion					801:813	lignocellulose bioconversion	786:813	lignocellulose bioconversion of white-rot fungus for the genus Pleurotus	786:857	The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus.					
30003974	3	41	theme	carbohydrate-active	475:493	arg1	CAZymes					504:510	CAZymes	504:510	CAZymes	504:510	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	3	41	theme	carbohydrate-active	475:493	arg1	enzymes					495:501	the carbohydrate-active enzymes	471:501	the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis	471:732	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
30003974	0	42	theme	species	59:65	arg1	genome					12:17	The diploid genome	0:17	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium -	0:114	The diploid genome of the only sclerotia-forming wild-type species in the genus Pleurotus -Pleurotus tuber-regium - provides insights into the mechanism of its biomass conversion from lignocellulose substrates.					
30003974	4	43	theme	white-rot	818:826	arg1	fungus					828:833	white-rot fungus	818:833	white-rot fungus	818:833	The present genomic data provides new insights for lignocellulose bioconversion of white-rot fungus for the genus Pleurotus.					
30003974	3	44	theme	enzymes	495:501	arg1	understanding					454:466	a better understanding	445:466	a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis	445:732	In this study, we sequenced and annotated the genome of a dikaryon P. tuber-regium wild strain to provide a better understanding of the carbohydrate-active enzymes (CAZymes) involved in the bio-conversion of lignocellulose to beta-glucan reserves in this sclerotia-forming Pleurotus mushroom with reference to enzyme participated in cellulosic compound breakdown and glucan reserve biosynthesis.					
29579941	6	0	from	flour	959:963	arg1	surfaces					908:915	the surfaces	904:915	the surfaces of the starch granules in the native rice flour	904:963	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	6	1	theme	granules	931:938	arg1	surfaces					908:915	the surfaces	904:915	the surfaces of the starch granules in the native rice flour	904:963	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	6	2	theme	native	947:952	arg1	flour					959:963	the native rice flour	943:963	the native rice flour	943:963	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	0	3	theme	rice	68:71	arg1	flour					73:77	rice flour	68:77	rice flour	68:77	Effect of endogenous proteins and lipids on starch digestibility in rice flour.					
29579941	9	4	theme	nutritional	1374:1384	arg1	profiles					1386:1393	improved nutritional profiles	1365:1393	improved nutritional profiles	1365:1393	These results have important implications for the design of foods with improved nutritional profiles.					
29579941	8	5	theme	down	1271:1274	arg1	digestion					1283:1291	slow down starch digestion	1266:1291	slow down starch digestion	1266:1291	In addition, amylose-lipid complex was detected in the rice flour, which is also known to slow down starch digestion.					
29579941	2	6	from	digestibility	267:279	arg1	flour					289:293	rice flour	284:293	rice flour	284:293	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	0	7	from	Effect	0:5	arg1	digestibility					51:63	starch digestibility	44:63	starch digestibility in rice flour	44:77	Effect of endogenous proteins and lipids on starch digestibility in rice flour.					
29579941	9	8	contain	have	1308:1311	arg1	results					1300:1306	These results	1294:1306	These results	1294:1306	These results have important implications for the design of foods with improved nutritional profiles.					
29579941	9	8	contain	have	1308:1311	arg2	implications					1323:1334	important implications	1313:1334	important implications for the design of foods with improved nutritional profiles	1313:1393	These results have important implications for the design of foods with improved nutritional profiles.					
29579941	6	9	from	surfaces	908:915	arg1	flour					959:963	the native rice flour	943:963	the native rice flour	943:963	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	3	10	dep	lipids	441:446	arg1	the					437:439	the	437:439	the	437:439	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	7	11	theme	starch	1085:1090	arg1	granules					1092:1099	the starch granules	1081:1099	the starch granules	1081:1099	Moreover, the proteins and lipids restricted the swelling of the starch granules, which may have decreased their digestion by reducing their surface areas.					
29579941	2	12	theme	proteins	237:244	arg1	effects					215:221	the effects	211:221	the effects of endogenous proteins and lipids on starch digestibility in rice flour	211:293	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	4	13	theme	starch	548:553	arg1	digestibility					555:567	starch digestibility	548:567	starch digestibility	548:567	A significant increase in starch digestibility was observed after removal of proteins, lipids, or both.					
29579941	1	14	contain	have	133:136	arg1	structure					100:108	structure	100:108	structure	100:108	The composition and structure of the food matrix can have a major impact on the digestion.					
29579941	1	14	contain	have	133:136	arg1	composition					84:94	composition	84:94	composition	84:94	The composition and structure of the food matrix can have a major impact on the digestion.					
29579941	1	14	contain	have	133:136	arg2	impact					146:151	a major impact	138:151	a major impact	138:151	The composition and structure of the food matrix can have a major impact on the digestion.					
29579941	8	15	theme	amylose-lipid	1189:1201	arg1	complex					1203:1209	amylose-lipid complex	1189:1209	amylose-lipid complex	1189:1209	In addition, amylose-lipid complex was detected in the rice flour, which is also known to slow down starch digestion.					
29579941	2	16	theme	endogenous	226:235	arg1	proteins					237:244	endogenous proteins	226:244	endogenous proteins	226:244	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	9	17	theme	improved	1365:1372	arg1	profiles					1386:1393	improved nutritional profiles	1365:1393	improved nutritional profiles	1365:1393	These results have important implications for the design of foods with improved nutritional profiles.					
29579941	1	18	theme	major	140:144	arg1	impact					146:151	a major impact	138:151	a major impact	138:151	The composition and structure of the food matrix can have a major impact on the digestion.					
29579941	4	19	from	increase	536:543	arg1	digestibility					555:567	starch digestibility	548:567	starch digestibility	548:567	A significant increase in starch digestibility was observed after removal of proteins, lipids, or both.					
29579941	6	20	from	granules	931:938	arg1	flour					959:963	the native rice flour	943:963	the native rice flour	943:963	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	3	21	theme	simulated	492:500	arg1	digestion					502:510	a simulated digestion	490:510	a simulated digestion	490:510	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	2	22	theme	underlying	333:342	arg1	mechanisms					360:369	the underlying physicochemical mechanisms	329:369	the underlying physicochemical mechanisms involved	329:378	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	5	23	theme	proteins	747:754	arg1	content					756:762	the proteins content	743:762	the proteins content	743:762	The starch digestibility of the rice flour without lipids was slightly lower than that without proteins, even though the proteins content was about 10-fold higher than the lipids content.					
29579941	5	23	theme	proteins	747:754	arg1	higher					782:787	higher	782:787	higher	782:787	The starch digestibility of the rice flour without lipids was slightly lower than that without proteins, even though the proteins content was about 10-fold higher than the lipids content.					
29579941	3	24	theme	long-grain	388:397	arg1	flour					411:415	Native long-grain indica rice flour	381:415	Native long-grain indica rice flour	381:415	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	5	25	theme	flour	663:667	arg1	digestibility					637:649	The starch digestibility	626:649	The starch digestibility of the rice flour without lipids	626:682	The starch digestibility of the rice flour without lipids was slightly lower than that without proteins, even though the proteins content was about 10-fold higher than the lipids content.					
29579941	5	25	theme	flour	663:667	arg1	lower					697:701	lower	697:701	lower	697:701	The starch digestibility of the rice flour without lipids was slightly lower than that without proteins, even though the proteins content was about 10-fold higher than the lipids content.					
29579941	6	26	theme	starch	924:929	arg1	granules					931:938	the starch granules	920:938	the starch granules in the native rice flour	920:963	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	8	27	located	detected	1215:1222	arg1	addition					1179:1186	addition	1179:1186	addition	1179:1186	In addition, amylose-lipid complex was detected in the rice flour, which is also known to slow down starch digestion.					
29579941	8	27	located	detected	1215:1222	arg2	complex					1203:1209	amylose-lipid complex	1189:1209	amylose-lipid complex	1189:1209	In addition, amylose-lipid complex was detected in the rice flour, which is also known to slow down starch digestion.					
29579941	8	27	located	detected	1215:1222	arg1	flour					1236:1240	the rice flour	1227:1240	the rice flour	1227:1240	In addition, amylose-lipid complex was detected in the rice flour, which is also known to slow down starch digestion.					
29579941	5	28	theme	lipids	798:803	arg1	content					805:811	the lipids content	794:811	the lipids content	794:811	The starch digestibility of the rice flour without lipids was slightly lower than that without proteins, even though the proteins content was about 10-fold higher than the lipids content.					
29579941	2	29	theme	starch	260:265	arg1	digestibility					267:279	starch digestibility	260:279	starch digestibility in rice flour	260:293	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	3	30	theme	indica	399:404	arg1	flour					411:415	Native long-grain indica rice flour	381:415	Native long-grain indica rice flour	381:415	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	0	31	theme	proteins	21:28	arg1	Effect					0:5	Effect	0:5	Effect of endogenous proteins and lipids on starch digestibility in rice flour.	0:78	Effect of endogenous proteins and lipids on starch digestibility in rice flour.					
29579941	8	32	theme	rice	1231:1234	arg1	flour					1236:1240	the rice flour	1227:1240	the rice flour	1227:1240	In addition, amylose-lipid complex was detected in the rice flour, which is also known to slow down starch digestion.					
29579941	9	33	theme	foods	1354:1358	arg1	design					1344:1349	the design	1340:1349	the design of foods with improved nutritional profiles	1340:1393	These results have important implications for the design of foods with improved nutritional profiles.					
29579941	4	34	theme	proteins	599:606	arg1	removal					588:594	removal	588:594	removal of proteins, lipids, or both	588:623	A significant increase in starch digestibility was observed after removal of proteins, lipids, or both.					
29579941	0	35	theme	endogenous	10:19	arg1	proteins					21:28	endogenous proteins	10:28	endogenous proteins	10:28	Effect of endogenous proteins and lipids on starch digestibility in rice flour.					
29579941	4	36	theme	significant	524:534	arg1	increase					536:543	A significant increase	522:543	A significant increase in starch digestibility	522:567	A significant increase in starch digestibility was observed after removal of proteins, lipids, or both.					
29579941	5	37	theme	rice	658:661	arg1	flour					663:667	the rice flour	654:667	the rice flour	654:667	The starch digestibility of the rice flour without lipids was slightly lower than that without proteins, even though the proteins content was about 10-fold higher than the lipids content.					
29579941	6	38	theme	Microstructural	814:828	arg1	analysis					830:837	Microstructural analysis	814:837	Microstructural analysis	814:837	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	2	39	theme	lipids	250:255	arg1	effects					215:221	the effects	211:221	the effects of endogenous proteins and lipids on starch digestibility in rice flour	211:293	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	2	40	from	effects	215:221	arg1	digestibility					267:279	starch digestibility	260:279	starch digestibility in rice flour	260:293	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	1	41	theme	matrix	122:127	arg1	structure					100:108	structure	100:108	structure	100:108	The composition and structure of the food matrix can have a major impact on the digestion.					
29579941	1	41	theme	matrix	122:127	arg1	composition					84:94	composition	84:94	composition	84:94	The composition and structure of the food matrix can have a major impact on the digestion.					
29579941	0	42	theme	lipids	34:39	arg1	Effect					0:5	Effect	0:5	Effect of endogenous proteins and lipids on starch digestibility in rice flour.	0:78	Effect of endogenous proteins and lipids on starch digestibility in rice flour.					
29579941	3	43	with	flour	426:430	arg1	proteins					455:462	proteins	455:462	proteins	455:462	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	3	43	with	flour	426:430	arg1	lipids					441:446	lipids	441:446	lipids	441:446	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	4	44	theme	both	620:623	arg1	removal					588:594	removal	588:594	removal of proteins, lipids, or both	588:623	A significant increase in starch digestibility was observed after removal of proteins, lipids, or both.					
29579941	4	45	theme	lipids	609:614	arg1	removal					588:594	removal	588:594	removal of proteins, lipids, or both	588:623	A significant increase in starch digestibility was observed after removal of proteins, lipids, or both.					
29579941	2	46	theme	physicochemical	344:358	arg1	mechanisms					360:369	the underlying physicochemical mechanisms	329:369	the underlying physicochemical mechanisms involved	329:378	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	3	47	theme	Native	381:386	arg1	flour					411:415	Native long-grain indica rice flour	381:415	Native long-grain indica rice flour	381:415	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	2	48	theme	work	187:190	arg1	aim					175:177	The aim	171:177	The aim of this work	171:190	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	3	49	theme	rice	421:424	arg1	flour					426:430	rice flour	421:430	rice flour	421:430	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	0	50	theme	starch	44:49	arg1	digestibility					51:63	starch digestibility	44:63	starch digestibility in rice flour	44:77	Effect of endogenous proteins and lipids on starch digestibility in rice flour.					
29579941	0	51	from	digestibility	51:63	arg1	flour					73:77	rice flour	68:77	rice flour	68:77	Effect of endogenous proteins and lipids on starch digestibility in rice flour.					
29579941	8	52	theme	starch	1276:1281	arg1	digestion					1283:1291	slow down starch digestion	1266:1291	slow down starch digestion	1266:1291	In addition, amylose-lipid complex was detected in the rice flour, which is also known to slow down starch digestion.					
29579941	5	53	theme	starch	630:635	arg1	digestibility					637:649	The starch digestibility	626:649	The starch digestibility of the rice flour without lipids	626:682	The starch digestibility of the rice flour without lipids was slightly lower than that without proteins, even though the proteins content was about 10-fold higher than the lipids content.					
29579941	5	53	theme	starch	630:635	arg1	lower					697:701	lower	697:701	lower	697:701	The starch digestibility of the rice flour without lipids was slightly lower than that without proteins, even though the proteins content was about 10-fold higher than the lipids content.					
29579941	6	54	attach	attached	892:899	arg2	lipids					871:876	lipids	871:876	lipids	871:876	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	6	54	attach	attached	892:899	arg2	proteins					858:865	proteins	858:865	proteins	858:865	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	6	54	attach	attached	892:899	arg1	surfaces					908:915	the surfaces	904:915	the surfaces of the starch granules in the native rice flour	904:963	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	3	55	theme	rice	406:409	arg1	flour					411:415	Native long-grain indica rice flour	381:415	Native long-grain indica rice flour	381:415	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	9	56	with	foods	1354:1358	arg1	profiles					1386:1393	improved nutritional profiles	1365:1393	improved nutritional profiles	1365:1393	These results have important implications for the design of foods with improved nutritional profiles.					
29579941	2	57	theme	rice	284:287	arg1	flour					289:293	rice flour	284:293	rice flour	284:293	The aim of this work was to investigate the effects of endogenous proteins and lipids on starch digestibility in rice flour, with an emphasis on establishing the underlying physicochemical mechanisms involved.					
29579941	8	58	theme	slow	1266:1269	arg1	digestion					1283:1291	slow down starch digestion	1266:1291	slow down starch digestion	1266:1291	In addition, amylose-lipid complex was detected in the rice flour, which is also known to slow down starch digestion.					
29579941	3	59	with	flour	411:415	arg1	proteins					455:462	proteins	455:462	proteins	455:462	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	3	59	with	flour	411:415	arg1	lipids					441:446	lipids	441:446	lipids	441:446	Native long-grain indica rice flour and rice flour with the lipids and/or proteins removed were subjected to a simulated digestion in vitro.					
29579941	6	60	theme	digestive	1001:1009	arg1	enzymes					1011:1017	digestive enzymes	1001:1017	digestive enzymes	1001:1017	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	1	61	theme	food	117:120	arg1	matrix					122:127	the food matrix	113:127	the food matrix	113:127	The composition and structure of the food matrix can have a major impact on the digestion.					
29579941	7	62	theme	surface	1161:1167	arg1	areas					1169:1173	their surface areas	1155:1173	their surface areas	1155:1173	Moreover, the proteins and lipids restricted the swelling of the starch granules, which may have decreased their digestion by reducing their surface areas.					
29579941	6	63	with	contact	988:994	arg1	enzymes					1011:1017	digestive enzymes	1001:1017	digestive enzymes	1001:1017	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
29579941	1	64	dep	composition	84:94	arg1	The					80:82	The	80:82	The	80:82	The composition and structure of the food matrix can have a major impact on the digestion.					
29579941	7	65	theme	granules	1092:1099	arg1	swelling					1069:1076	the swelling	1065:1076	the swelling	1065:1076	Moreover, the proteins and lipids restricted the swelling of the starch granules, which may have decreased their digestion by reducing their surface areas.					
29579941	9	66	theme	important	1313:1321	arg1	implications					1323:1334	important implications	1313:1334	important implications for the design of foods with improved nutritional profiles	1313:1393	These results have important implications for the design of foods with improved nutritional profiles.					
29579941	6	67	theme	rice	954:957	arg1	flour					959:963	the native rice flour	943:963	the native rice flour	943:963	Microstructural analysis suggested that the proteins and lipids were normally attached to the surfaces of the starch granules in the native rice flour, thus inhibiting their contact with digestive enzymes.					
31675580	6	0	theme	independent	982:992	arg1	structure					1009:1017	The independent triple-network structure	978:1017	The independent triple-network structure	978:1017	The independent triple-network structure increases the three-dimensional space, so there are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin.					
31675580	12	1	theme	low	1909:1911	arg1	temperature					1913:1923	low temperature	1909:1923	low temperature	1909:1923	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	7	2	theme	hydrogels	1217:1225	arg1	capacity					1188:1195	the adsorption capacity	1173:1195	the adsorption capacity of CNTs/L-cys@GO/SA hydrogels	1173:1225	And the adsorption capacity of CNTs/L-cys@GO/SA hydrogels can reach 181 mg g-1 and 200 mg g-1 at 25 °C and 15 °C respectively in weak acidity environment.					
31675580	3	3	theme	other	517:521	arg1	properties					523:532	other properties	517:532	other properties	517:532	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	10	4	theme	larger	1675:1680	arg1	hydrogels					1665:1673	hydrogels	1665:1673	hydrogels larger	1665:1680	The independent multilayer network can retain the excellent properties of the original materials and make the internal space of hydrogels larger.					
31675580	7	5	theme	@	1210:1210	arg1	hydrogels					1217:1225	CNTs/L-cys@GO/SA hydrogels	1200:1225	CNTs/L-cys@GO/SA hydrogels	1200:1225	And the adsorption capacity of CNTs/L-cys@GO/SA hydrogels can reach 181 mg g-1 and 200 mg g-1 at 25 °C and 15 °C respectively in weak acidity environment.					
31675580	2	6	theme	adsorption	310:319	arg1	performance					321:331	poor adsorption performance	305:331	poor adsorption performance	305:331	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	5	7	theme	three-dimensional	912:928	arg1	structure					930:938	their three-dimensional structure to improve their adsorption capacity	906:975	their three-dimensional structure to improve their adsorption capacity	906:975	Compared with traditional hydrogels and the double-network hydrogels that are currently being developed, these triple-network composite hydrogels can exploit their three-dimensional structure to improve their adsorption capacity.					
31675580	4	8	theme	@	690:690	arg1	GO/SA					691:695	CNTs/L-cys@GO/SA	680:695	CNTs/L-cys@GO/SA	680:695	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	4	8	theme	@	690:690	arg1	alginate					670:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate	620:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	7	9	theme	adsorption	1177:1186	arg1	capacity					1188:1195	the adsorption capacity	1173:1195	the adsorption capacity of CNTs/L-cys@GO/SA hydrogels	1173:1225	And the adsorption capacity of CNTs/L-cys@GO/SA hydrogels can reach 181 mg g-1 and 200 mg g-1 at 25 °C and 15 °C respectively in weak acidity environment.					
31675580	1	10	theme	environmental	215:227	arg1	problems					229:236	serious environmental problems	207:236	serious environmental problems	207:236	Large-scale abuse of antibiotics has led to serious environmental problems.					
31675580	12	11	theme	ciprofloxacin	1857:1869	arg1	capacity					1845:1852	the higher adsorption capacity	1823:1852	the higher adsorption capacity of ciprofloxacin	1823:1869	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	4	12	theme	@	647:647	arg1	GO/SA					691:695	CNTs/L-cys@GO/SA	680:695	CNTs/L-cys@GO/SA	680:695	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	4	12	theme	@	647:647	arg1	alginate					670:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate	620:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	0	13	theme	aqueous	144:150	arg1	solutions					152:160	aqueous solutions	144:160	aqueous solutions	144:160	Enhanced adsorption for the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network nanocomposite hydrogels in aqueous solutions.					
31675580	2	14	theme	mechanical	348:357	arg1	properties					359:368	inadequate mechanical properties	337:368	inadequate mechanical properties	337:368	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	8	15	theme	better	1365:1370	arg1	property					1372:1379	better property	1365:1379	better property	1365:1379	In fact, CNTs/L-cys@GO/SA hydrogels show better property at low temperature.					
31675580	0	16	from	hydrogels	131:139	arg1	solutions					152:160	aqueous solutions	144:160	aqueous solutions	144:160	Enhanced adsorption for the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network nanocomposite hydrogels in aqueous solutions.					
31675580	6	17	dep	space	1051:1055	arg1	are					1067:1069	are	1067:1069	are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin	1067:1166	The independent triple-network structure increases the three-dimensional space, so there are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin.					
31675580	1	18	theme	Large-scale	163:173	arg1	abuse					175:179	Large-scale abuse	163:179	Large-scale abuse of antibiotics	163:194	Large-scale abuse of antibiotics has led to serious environmental problems.					
31675580	12	19	theme	adsorption	1834:1843	arg1	capacity					1845:1852	the higher adsorption capacity	1823:1852	the higher adsorption capacity of ciprofloxacin	1823:1869	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	0	20	theme	carbon	54:59	arg1	triple-network					102:115	carbon nanotubes/graphene oxide/sodium alginate triple-network	54:115	carbon nanotubes/graphene oxide/sodium alginate triple-network	54:115	Enhanced adsorption for the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network nanocomposite hydrogels in aqueous solutions.					
31675580	2	21	theme	biopolymer	284:293	arg1	gels					295:298	several biopolymer gels	276:298	several biopolymer gels	276:298	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	12	22	theme	@	1801:1801	arg1	hydrogels					1808:1816	the CNTs/L-cys@GO/SA hydrogels	1787:1816	the CNTs/L-cys@GO/SA hydrogels	1787:1816	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	6	23	theme	pollutant	1086:1094	arg1	sites					1107:1111	pollutant adsorption sites	1086:1111	pollutant adsorption sites	1086:1111	The independent triple-network structure increases the three-dimensional space, so there are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin.					
31675580	10	24	theme	independent	1541:1551	arg1	network					1564:1570	The independent multilayer network	1537:1570	The independent multilayer network	1537:1570	The independent multilayer network can retain the excellent properties of the original materials and make the internal space of hydrogels larger.					
31675580	4	25	theme	composite	713:721	arg1	hydrogels					723:731	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	0	26	theme	oxide/sodium	80:91	arg1	triple-network					102:115	carbon nanotubes/graphene oxide/sodium alginate triple-network	54:115	carbon nanotubes/graphene oxide/sodium alginate triple-network	54:115	Enhanced adsorption for the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network nanocomposite hydrogels in aqueous solutions.					
31675580	3	27	dep	oxide	423:427	arg1	GO					430:431	GO	430:431	GO	430:431	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	7	28	theme	acidity	1303:1309	arg1	environment					1311:1321	weak acidity environment	1298:1321	weak acidity environment	1298:1321	And the adsorption capacity of CNTs/L-cys@GO/SA hydrogels can reach 181 mg g-1 and 200 mg g-1 at 25 °C and 15 °C respectively in weak acidity environment.					
31675580	7	29	theme	weak	1298:1301	arg1	acidity					1303:1309	weak acidity	1298:1309	weak acidity environment	1298:1321	And the adsorption capacity of CNTs/L-cys@GO/SA hydrogels can reach 181 mg g-1 and 200 mg g-1 at 25 °C and 15 °C respectively in weak acidity environment.					
31675580	3	30	theme	sodium	454:459	arg1	SA					471:472	SA	471:472	SA	471:472	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	3	30	theme	sodium	454:459	arg1	alginate					461:468	sodium alginate	454:468	sodium alginate (SA)	454:473	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	3	31	dep	performance	501:511	arg1	the					486:488	the	486:488	the	486:488	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	0	32	theme	Enhanced	0:7	arg1	adsorption					9:18	Enhanced adsorption	0:18	Enhanced adsorption for the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network	0:115	Enhanced adsorption for the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network nanocomposite hydrogels in aqueous solutions.					
31675580	5	33	theme	triple-network	859:872	arg1	hydrogels					884:892	these triple-network composite hydrogels	853:892	these triple-network composite hydrogels	853:892	Compared with traditional hydrogels and the double-network hydrogels that are currently being developed, these triple-network composite hydrogels can exploit their three-dimensional structure to improve their adsorption capacity.					
31675580	6	34	theme	high-efficient	1128:1141	arg1	removal					1143:1149	the high-efficient removal	1124:1149	the high-efficient removal of ciprofloxacin	1124:1166	The independent triple-network structure increases the three-dimensional space, so there are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin.					
31675580	10	35	theme	excellent	1587:1595	arg1	properties					1597:1606	the excellent properties	1583:1606	the excellent properties of the original materials	1583:1632	The independent multilayer network can retain the excellent properties of the original materials and make the internal space of hydrogels larger.					
31675580	9	36	theme	mechanical	1437:1446	arg1	properties					1448:1457	mechanical properties	1437:1457	mechanical properties	1437:1457	In addition, the thermal stability, mechanical properties and swelling ability of the triple-network hydrogels have also been improved.					
31675580	12	37	theme	ciprofloxacin	1981:1993	arg1	removal					1970:1976	the removal	1966:1976	the removal of ciprofloxacin by hydrogels	1966:2006	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	12	38	theme	low	1929:1931	arg1	concentration					1948:1960	low inorganic salt concentration	1929:1960	low inorganic salt concentration	1929:1960	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	3	39	theme	adsorption	490:499	arg1	performance					501:511	adsorption performance	490:511	adsorption performance	490:511	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	0	40	theme	antibiotics	39:49	arg1	removal					28:34	the removal	24:34	the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network	24:115	Enhanced adsorption for the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network nanocomposite hydrogels in aqueous solutions.					
31675580	12	41	theme	concentration	1948:1960	arg1	conditions					1881:1890	the conditions	1877:1890	the conditions of weak acidity, low temperature and low inorganic salt concentration	1877:1960	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	12	42	theme	salt	1943:1946	arg1	concentration					1948:1960	low inorganic salt concentration	1929:1960	low inorganic salt concentration	1929:1960	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	10	43	theme	original	1615:1622	arg1	materials					1624:1632	the original materials	1611:1632	the original materials	1611:1632	The independent multilayer network can retain the excellent properties of the original materials and make the internal space of hydrogels larger.					
31675580	11	44	theme	multinetwork	1689:1700	arg1	hydrogels					1702:1710	These multinetwork hydrogels	1683:1710	These multinetwork hydrogels	1683:1710	These multinetwork hydrogels have great potential for removing pollutants from wastewater.					
31675580	3	45	theme	adsorbents	549:558	arg1	performance					501:511	adsorption performance	490:511	adsorption performance	490:511	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	3	45	theme	adsorbents	549:558	arg1	properties					523:532	other properties	517:532	other properties	517:532	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	6	46	theme	triple-network	994:1007	arg1	structure					1009:1017	The independent triple-network structure	978:1017	The independent triple-network structure	978:1017	The independent triple-network structure increases the three-dimensional space, so there are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin.					
31675580	12	47	theme	temperature	1913:1923	arg1	conditions					1881:1890	the conditions	1877:1890	the conditions of weak acidity, low temperature and low inorganic salt concentration	1877:1960	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	5	48	theme	double-network	792:805	arg1	hydrogels					807:815	the double-network hydrogels	788:815	the double-network hydrogels	788:815	Compared with traditional hydrogels and the double-network hydrogels that are currently being developed, these triple-network composite hydrogels can exploit their three-dimensional structure to improve their adsorption capacity.					
31675580	7	49	theme	GO/SA	1211:1215	arg1	hydrogels					1217:1225	CNTs/L-cys@GO/SA hydrogels	1200:1225	CNTs/L-cys@GO/SA hydrogels	1200:1225	And the adsorption capacity of CNTs/L-cys@GO/SA hydrogels can reach 181 mg g-1 and 200 mg g-1 at 25 °C and 15 °C respectively in weak acidity environment.					
31675580	9	50	theme	hydrogels	1502:1510	arg1	stability					1426:1434	thermal stability	1418:1434	thermal stability	1418:1434	In addition, the thermal stability, mechanical properties and swelling ability of the triple-network hydrogels have also been improved.					
31675580	9	50	theme	hydrogels	1502:1510	arg1	properties					1448:1457	mechanical properties	1437:1457	mechanical properties	1437:1457	In addition, the thermal stability, mechanical properties and swelling ability of the triple-network hydrogels have also been improved.					
31675580	9	50	theme	hydrogels	1502:1510	arg1	ability					1472:1478	swelling ability	1463:1478	swelling ability	1463:1478	In addition, the thermal stability, mechanical properties and swelling ability of the triple-network hydrogels have also been improved.					
31675580	7	51	theme	CNTs/L-cys	1200:1209	arg1	hydrogels					1217:1225	CNTs/L-cys@GO/SA hydrogels	1200:1225	CNTs/L-cys@GO/SA hydrogels	1200:1225	And the adsorption capacity of CNTs/L-cys@GO/SA hydrogels can reach 181 mg g-1 and 200 mg g-1 at 25 °C and 15 °C respectively in weak acidity environment.					
31675580	2	52	theme	inadequate	337:346	arg1	properties					359:368	inadequate mechanical properties	337:368	inadequate mechanical properties	337:368	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	2	53	contain	have	300:303	arg1	gels					295:298	several biopolymer gels	276:298	several biopolymer gels	276:298	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	2	53	contain	have	300:303	arg1	adsorbents					257:266	Some conventional adsorbents	239:266	Some conventional adsorbents such as several biopolymer gels	239:298	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	2	53	contain	have	300:303	arg2	properties					359:368	inadequate mechanical properties	337:368	inadequate mechanical properties	337:368	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	2	53	contain	have	300:303	arg2	performance					321:331	poor adsorption performance	305:331	poor adsorption performance	305:331	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	12	54	theme	weak	1895:1898	arg1	acidity					1900:1906	weak acidity	1895:1906	weak acidity	1895:1906	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	5	55	theme	adsorption	957:966	arg1	capacity					968:975	their adsorption capacity	951:975	their adsorption capacity	951:975	Compared with traditional hydrogels and the double-network hydrogels that are currently being developed, these triple-network composite hydrogels can exploit their three-dimensional structure to improve their adsorption capacity.					
31675580	10	56	theme	hydrogels	1665:1673	arg1	space					1656:1660	the internal space	1643:1660	the internal space of hydrogels larger	1643:1680	The independent multilayer network can retain the excellent properties of the original materials and make the internal space of hydrogels larger.					
31675580	3	57	theme	traditional	537:547	arg1	adsorbents					549:558	traditional adsorbents	537:558	traditional adsorbents	537:558	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	8	58	theme	@	1343:1343	arg1	hydrogels					1350:1358	CNTs/L-cys@GO/SA hydrogels	1333:1358	CNTs/L-cys@GO/SA hydrogels	1333:1358	In fact, CNTs/L-cys@GO/SA hydrogels show better property at low temperature.					
31675580	4	59	theme	alginate	670:677	arg1	hydrogels					723:731	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	1	60	theme	serious	207:213	arg1	problems					229:236	serious environmental problems	207:236	serious environmental problems	207:236	Large-scale abuse of antibiotics has led to serious environmental problems.					
31675580	8	61	theme	CNTs/L-cys	1333:1342	arg1	hydrogels					1350:1358	CNTs/L-cys@GO/SA hydrogels	1333:1358	CNTs/L-cys@GO/SA hydrogels	1333:1358	In fact, CNTs/L-cys@GO/SA hydrogels show better property at low temperature.					
31675580	5	62	theme	composite	874:882	arg1	hydrogels					884:892	these triple-network composite hydrogels	853:892	these triple-network composite hydrogels	853:892	Compared with traditional hydrogels and the double-network hydrogels that are currently being developed, these triple-network composite hydrogels can exploit their three-dimensional structure to improve their adsorption capacity.					
31675580	8	63	theme	GO/SA	1344:1348	arg1	hydrogels					1350:1358	CNTs/L-cys@GO/SA hydrogels	1333:1358	CNTs/L-cys@GO/SA hydrogels	1333:1358	In fact, CNTs/L-cys@GO/SA hydrogels show better property at low temperature.					
31675580	2	64	theme	several	276:282	arg1	gels					295:298	several biopolymer gels	276:298	several biopolymer gels	276:298	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	4	65	theme	CNTs/L-cys	680:689	arg1	GO/SA					691:695	CNTs/L-cys@GO/SA	680:695	CNTs/L-cys@GO/SA	680:695	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	4	65	theme	CNTs/L-cys	680:689	arg1	alginate					670:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate	620:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	6	66	theme	three-dimensional	1033:1049	arg1	space					1051:1055	the three-dimensional space	1029:1055	the three-dimensional space	1029:1055	The independent triple-network structure increases the three-dimensional space, so there are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin.					
31675580	4	67	theme	graphene	648:655	arg1	GO/SA					691:695	CNTs/L-cys@GO/SA	680:695	CNTs/L-cys@GO/SA	680:695	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	4	67	theme	graphene	648:655	arg1	alginate					670:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate	620:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	11	68	contain	have	1712:1715	arg1	hydrogels					1702:1710	These multinetwork hydrogels	1683:1710	These multinetwork hydrogels	1683:1710	These multinetwork hydrogels have great potential for removing pollutants from wastewater.					
31675580	11	68	contain	have	1712:1715	arg2	potential					1723:1731	great potential	1717:1731	great potential	1717:1731	These multinetwork hydrogels have great potential for removing pollutants from wastewater.					
31675580	2	69	theme	poor	305:308	arg1	performance					321:331	poor adsorption performance	305:331	poor adsorption performance	305:331	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	8	70	theme	low	1384:1386	arg1	temperature					1388:1398	low temperature	1384:1398	low temperature	1384:1398	In fact, CNTs/L-cys@GO/SA hydrogels show better property at low temperature.					
31675580	0	71	theme	nanotubes/graphene	61:78	arg1	triple-network					102:115	carbon nanotubes/graphene oxide/sodium alginate triple-network	54:115	carbon nanotubes/graphene oxide/sodium alginate triple-network	54:115	Enhanced adsorption for the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network nanocomposite hydrogels in aqueous solutions.					
31675580	12	72	theme	higher	1827:1832	arg1	capacity					1845:1852	the higher adsorption capacity	1823:1852	the higher adsorption capacity of ciprofloxacin	1823:1869	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	6	73	theme	adsorption	1096:1105	arg1	sites					1107:1111	pollutant adsorption sites	1086:1111	pollutant adsorption sites	1086:1111	The independent triple-network structure increases the three-dimensional space, so there are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin.					
31675580	3	74	theme	carbon	386:391	arg1	CNTs					404:407	CNTs	404:407	CNTs	404:407	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	3	74	theme	carbon	386:391	arg1	nanotubes					393:401	carbon nanotubes	386:401	carbon nanotubes (CNTs)	386:408	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	9	75	dep	stability	1426:1434	arg1	the					1414:1416	the	1414:1416	the	1414:1416	In addition, the thermal stability, mechanical properties and swelling ability of the triple-network hydrogels have also been improved.					
31675580	0	76	theme	alginate	93:100	arg1	triple-network					102:115	carbon nanotubes/graphene oxide/sodium alginate triple-network	54:115	carbon nanotubes/graphene oxide/sodium alginate triple-network	54:115	Enhanced adsorption for the removal of antibiotics by carbon nanotubes/graphene oxide/sodium alginate triple-network nanocomposite hydrogels in aqueous solutions.					
31675580	1	77	theme	antibiotics	184:194	arg1	abuse					175:179	Large-scale abuse	163:179	Large-scale abuse of antibiotics	163:194	Large-scale abuse of antibiotics has led to serious environmental problems.					
31675580	12	78	theme	CNTs/L-cys	1791:1800	arg1	hydrogels					1808:1816	the CNTs/L-cys@GO/SA hydrogels	1787:1816	the CNTs/L-cys@GO/SA hydrogels	1787:1816	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	4	79	theme	triple-network	698:711	arg1	hydrogels					723:731	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	12	80	theme	environmental	2041:2053	arg1	conditions					2055:2064	environmental conditions	2041:2064	environmental conditions	2041:2064	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	12	81	theme	GO/SA	1802:1806	arg1	hydrogels					1808:1816	the CNTs/L-cys@GO/SA hydrogels	1787:1816	the CNTs/L-cys@GO/SA hydrogels	1787:1816	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	6	82	theme	more	1071:1074	arg1	pores					1076:1080	more pores	1071:1080	more pores	1071:1080	The independent triple-network structure increases the three-dimensional space, so there are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin.					
31675580	5	83	theme	traditional	762:772	arg1	hydrogels					774:782	traditional hydrogels	762:782	traditional hydrogels	762:782	Compared with traditional hydrogels and the double-network hydrogels that are currently being developed, these triple-network composite hydrogels can exploit their three-dimensional structure to improve their adsorption capacity.					
31675580	4	84	theme	nanotubes/l-cysteine	627:646	arg1	GO/SA					691:695	CNTs/L-cys@GO/SA	680:695	CNTs/L-cys@GO/SA	680:695	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	4	84	theme	nanotubes/l-cysteine	627:646	arg1	alginate					670:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate	620:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	4	85	dep	the	566:568	arg1	help					570:573	help	570:573	help	570:573	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	6	86	theme	ciprofloxacin	1154:1166	arg1	removal					1143:1149	the high-efficient removal	1124:1149	the high-efficient removal of ciprofloxacin	1124:1166	The independent triple-network structure increases the three-dimensional space, so there are more pores and pollutant adsorption sites to achieve the high-efficient removal of ciprofloxacin.					
31675580	10	87	theme	multilayer	1553:1562	arg1	network					1564:1570	The independent multilayer network	1537:1570	The independent multilayer network	1537:1570	The independent multilayer network can retain the excellent properties of the original materials and make the internal space of hydrogels larger.					
31675580	9	88	theme	thermal	1418:1424	arg1	stability					1426:1434	thermal stability	1418:1434	thermal stability	1418:1434	In addition, the thermal stability, mechanical properties and swelling ability of the triple-network hydrogels have also been improved.					
31675580	3	89	theme	graphene	414:421	arg1	oxide					423:427	graphene oxide	414:427	graphene oxide (GO)	414:432	In this paper, carbon nanotubes (CNTs) and graphene oxide (GO), were combined with sodium alginate (SA) to improve the adsorption performance and other properties of traditional adsorbents.					
31675580	4	90	theme	hydrogen	578:585	arg1	peroxide					587:594	hydrogen peroxide	578:594	hydrogen peroxide	578:594	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	4	91	theme	oxide/sodium	657:668	arg1	GO/SA					691:695	CNTs/L-cys@GO/SA	680:695	CNTs/L-cys@GO/SA	680:695	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	4	91	theme	oxide/sodium	657:668	arg1	alginate					670:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate	620:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	10	92	theme	materials	1624:1632	arg1	properties					1597:1606	the excellent properties	1583:1606	the excellent properties of the original materials	1583:1632	The independent multilayer network can retain the excellent properties of the original materials and make the internal space of hydrogels larger.					
31675580	2	93	theme	conventional	244:255	arg1	gels					295:298	several biopolymer gels	276:298	several biopolymer gels	276:298	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	2	93	theme	conventional	244:255	arg1	adsorbents					257:266	Some conventional adsorbents	239:266	Some conventional adsorbents such as several biopolymer gels	239:298	Some conventional adsorbents such as several biopolymer gels have poor adsorption performance and inadequate mechanical properties.					
31675580	9	94	theme	swelling	1463:1470	arg1	ability					1472:1478	swelling ability	1463:1478	swelling ability	1463:1478	In addition, the thermal stability, mechanical properties and swelling ability of the triple-network hydrogels have also been improved.					
31675580	12	95	theme	inorganic	1933:1941	arg1	concentration					1948:1960	low inorganic salt concentration	1929:1960	low inorganic salt concentration	1929:1960	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	12	96	theme	acidity	1900:1906	arg1	conditions					1881:1890	the conditions	1877:1890	the conditions of weak acidity, low temperature and low inorganic salt concentration	1877:1960	In addition, the CNTs/L-cys@GO/SA hydrogels show the higher adsorption capacity of ciprofloxacin under the conditions of weak acidity, low temperature and low inorganic salt concentration, so the removal of ciprofloxacin by hydrogels can also be promoted by changing environmental conditions.					
31675580	4	97	theme	carbon	620:625	arg1	GO/SA					691:695	CNTs/L-cys@GO/SA	680:695	CNTs/L-cys@GO/SA	680:695	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	4	97	theme	carbon	620:625	arg1	alginate					670:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate	620:677	carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels	620:731	With the help of hydrogen peroxide and l-cysteine (L-cys), carbon nanotubes/l-cysteine@graphene oxide/sodium alginate (CNTs/L-cys@GO/SA) triple-network composite hydrogels were prepared.					
31675580	10	98	theme	internal	1647:1654	arg1	space					1656:1660	the internal space	1643:1660	the internal space of hydrogels larger	1643:1680	The independent multilayer network can retain the excellent properties of the original materials and make the internal space of hydrogels larger.					
31675580	11	99	theme	great	1717:1721	arg1	potential					1723:1731	great potential	1717:1731	great potential	1717:1731	These multinetwork hydrogels have great potential for removing pollutants from wastewater.					
31675580	9	100	theme	triple-network	1487:1500	arg1	hydrogels					1502:1510	the triple-network hydrogels	1483:1510	the triple-network hydrogels	1483:1510	In addition, the thermal stability, mechanical properties and swelling ability of the triple-network hydrogels have also been improved.					
30440084	7	0	theme	pressure	1193:1200	arg1	powders					1227:1233	high pressure and low pressure treated powders	1188:1233	high pressure and low pressure treated powders	1188:1233	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	8	1	theme	spray	1387:1391	arg1	drying					1393:1398	spray drying	1387:1398	spray drying	1387:1398	PRACTICAL APPLICATION: Drying of camel milk (using spray drying) can extend its shelf life.					
30440084	2	2	theme	vitamin	416:422	arg1	recoveries					426:435	vitamin C recoveries	416:435	vitamin C recoveries	416:435	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	7	3	theme	profiles	1136:1143	arg1	MCA					1114:1116	the MCA	1110:1116	the MCA of the fatty acid profiles of camel milk powders	1110:1165	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	9	4	theme	powder	1578:1583	arg1	properties					1506:1515	the nutritional properties	1490:1515	the nutritional properties of the milk	1490:1527	The spray drying process must be optimized in order to retain the nutritional properties of the milk and the techno-functional characteristics of the powder.					
30440084	9	4	theme	powder	1578:1583	arg1	characteristics					1555:1569	the techno-functional characteristics	1533:1569	the techno-functional characteristics of the powder	1533:1583	The spray drying process must be optimized in order to retain the nutritional properties of the milk and the techno-functional characteristics of the powder.					
30440084	3	5	theme	powders	539:545	arg1	classification					492:505	classification	492:505	classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles	492:652	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	6	6	theme	High	913:916	arg1	temperature					925:935	High outlet temperature	913:935	High outlet temperature	913:935	High outlet temperature and pressure decreased vitamin C recovery.					
30440084	3	7	theme	camel	528:532	arg1	powders					539:545	spray dried whole camel milk powders	510:545	spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles	510:652	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	1	8	theme	drying	139:144	arg1	effect					123:128	The effect	119:128	The effect of spray drying on the composition of camel milk powder	119:184	The effect of spray drying on the composition of camel milk powder was evaluated.					
30440084	7	9	theme	milk	1154:1157	arg1	powders					1159:1165	camel milk powders	1148:1165	camel milk powders	1148:1165	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	4	10	theme	proximate	667:675	arg1	%					760:760	3.0%	757:760	3.0%	757:760	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	4	10	theme	proximate	667:675	arg1	composition					677:687	The average proximate composition	655:687	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid	655:750	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	11	11	dep	is	1760:1761	arg1	need					1763:1766	need	1763:1766	is need to translate these findings to an industrial volume dryer	1760:1824	However, there is need to translate these findings to an industrial volume dryer.					
30440084	3	12	theme	processing	562:571	arg1	conditions					573:582	their processing conditions	556:582	their processing conditions	556:582	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	8	13	theme	shelf	1416:1420	arg1	life					1422:1425	its shelf life	1412:1425	its shelf life	1412:1425	PRACTICAL APPLICATION: Drying of camel milk (using spray drying) can extend its shelf life.					
30440084	4	14	theme	protein	718:724	arg1	%					760:760	3.0%	757:760	3.0%	757:760	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	4	14	theme	protein	718:724	arg1	composition					677:687	The average proximate composition	655:687	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid	655:750	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	6	15	theme	C	968:968	arg1	recovery					970:977	vitamin C recovery	960:977	vitamin C recovery	960:977	High outlet temperature and pressure decreased vitamin C recovery.					
30440084	10	16	theme	conditions	1623:1632	arg1	effect					1596:1601	the effect	1592:1601	the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder	1592:1700	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	0	17	theme	Whole	94:98	arg1	Powder					111:116	Whole Camel Milk Powder	94:116	Whole Camel Milk Powder	94:116	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	5	18	theme	powder	806:811	arg1	yield					813:817	The powder yield	802:817	The powder yield (cyclone recovery)	802:836	The powder yield (cyclone recovery) increased with increasing inlet temperature and decreasing feed flow rate.					
30440084	5	18	theme	powder	806:811	arg1	recovery					828:835	cyclone recovery	820:835	cyclone recovery	820:835	The powder yield (cyclone recovery) increased with increasing inlet temperature and decreasing feed flow rate.					
30440084	4	19	theme	lactose	727:733	arg1	%					760:760	3.0%	757:760	3.0%	757:760	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	4	19	theme	lactose	727:733	arg1	composition					677:687	The average proximate composition	655:687	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid	655:750	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	12	20	theme	food	1973:1976	arg1	processing					1978:1987	food processing	1973:1987	food processing	1973:1987	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	10	21	theme	nutritional	1660:1670	arg1	components					1672:1681	selected nutritional components	1651:1681	selected nutritional components	1651:1681	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	3	22	theme	correspondence	597:610	arg1	MCA					622:624	MCA	622:624	MCA	622:624	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	3	22	theme	correspondence	597:610	arg1	analysis					612:619	multiple correspondence analysis	588:619	multiple correspondence analysis (MCA)	588:625	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	0	23	theme	Milk	106:109	arg1	Powder					111:116	Whole Camel Milk Powder	94:116	Whole Camel Milk Powder	94:116	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	1	24	theme	milk	174:177	arg1	powder					179:184	camel milk powder	168:184	camel milk powder	168:184	The effect of spray drying on the composition of camel milk powder was evaluated.					
30440084	7	25	theme	fatty	1323:1327	arg1	acids					1329:1333	fatty acids	1323:1333	fatty acids	1323:1333	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	4	26	theme	viz.	707:710	arg1	%					760:760	3.0%	757:760	3.0%	757:760	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	4	26	theme	viz.	707:710	arg1	composition					677:687	The average proximate composition	655:687	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid	655:750	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	7	27	theme	treated	1083:1089	arg1	powders					1096:1102	high heat treated and low heat treated milk powders	1052:1102	high heat treated and low heat treated milk powders	1052:1102	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	4	28	theme	camel	696:700	arg1	viz.					707:710	the camel milk viz.	692:710	the camel milk viz.	692:710	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	4	29	theme	fat	713:715	arg1	%					760:760	3.0%	757:760	3.0%	757:760	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	4	29	theme	fat	713:715	arg1	composition					677:687	The average proximate composition	655:687	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid	655:750	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	2	30	theme	experimental	218:229	arg1	design					231:236	A full factorial experimental design	201:236	A full factorial experimental design	201:236	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	7	31	theme	low	1074:1076	arg1	powders					1096:1102	high heat treated and low heat treated milk powders	1052:1102	high heat treated and low heat treated milk powders	1052:1102	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	10	32	theme	milk	1690:1693	arg1	powder					1695:1700	the milk powder	1686:1700	the milk powder	1686:1700	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	2	33	theme	factorial	208:216	arg1	design					231:236	A full factorial experimental design	201:236	A full factorial experimental design	201:236	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	2	34	theme	flow	371:374	arg1	rev/s					394:398	5, 4, and 3 rev/s	382:398	5, 4, and 3 rev/s	382:398	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	2	34	theme	flow	371:374	arg1	rate					376:379	feed flow rate	366:379	feed flow rate (5, 4, and 3 rev/s)	366:399	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	7	35	theme	treated	1062:1068	arg1	powders					1096:1102	high heat treated and low heat treated milk powders	1052:1102	high heat treated and low heat treated milk powders	1052:1102	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	8	36	dep	APPLICATION	1346:1356	arg1	extend					1405:1410	extend	1405:1410	can extend its shelf life	1401:1425	PRACTICAL APPLICATION: Drying of camel milk (using spray drying) can extend its shelf life.					
30440084	3	37	theme	Principal	438:446	arg1	PCA					469:471	PCA	469:471	PCA	469:471	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	3	37	theme	Principal	438:446	arg1	analysis					459:466	Principal components analysis	438:466	Principal components analysis (PCA)	438:472	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	9	38	theme	drying	1438:1443	arg1	process					1445:1451	The spray drying process	1428:1451	The spray drying process	1428:1451	The spray drying process must be optimized in order to retain the nutritional properties of the milk and the techno-functional characteristics of the powder.					
30440084	10	39	dep	yield	1641:1645	arg1	the					1637:1639	the	1637:1639	the	1637:1639	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	10	40	theme	spray	1610:1614	arg1	conditions					1623:1632	the spray drying conditions	1606:1632	the spray drying conditions	1606:1632	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	3	41	theme	acid	640:643	arg1	profiles					645:652	the fatty acid profiles	630:652	the fatty acid profiles	630:652	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	1	42	from	effect	123:128	arg1	composition					153:163	the composition	149:163	the composition of camel milk powder	149:184	The effect of spray drying on the composition of camel milk powder was evaluated.					
30440084	7	43	theme	atomization	1268:1278	arg1	pressure					1280:1287	higher atomization pressure	1261:1287	higher atomization pressure	1261:1287	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	12	44	theme	components	1938:1947	arg1	retention					1903:1911	retention	1903:1911	retention of important nutritional components	1903:1947	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	12	44	theme	components	1938:1947	arg1	part					1965:1968	an important part	1952:1968	an important part of food processing	1952:1987	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	12	45	theme	future	1866:1871	arg1	operations					1886:1895	future spray drying operations	1866:1895	future spray drying operations	1866:1895	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	0	46	theme	Selected	59:66	arg1	Components					80:89	Selected Nutritional Components	59:89	Selected Nutritional Components	59:89	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	4	47	theme	solid	746:750	arg1	%					760:760	3.0%	757:760	3.0%	757:760	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	4	47	theme	solid	746:750	arg1	composition					677:687	The average proximate composition	655:687	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid	655:750	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	12	48	theme	drying	1879:1884	arg1	operations					1886:1895	future spray drying operations	1866:1895	future spray drying operations	1866:1895	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	11	49	theme	volume	1813:1818	arg1	dryer					1820:1824	an industrial volume dryer	1799:1824	an industrial volume dryer	1799:1824	However, there is need to translate these findings to an industrial volume dryer.					
30440084	2	50	dep	temperature	275:285	arg1	°C					312:313	120 °C	308:313	120 °C	308:313	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	2	50	dep	temperature	275:285	arg1	°C					292:293	160 °C	288:293	160 °C	288:293	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	2	50	dep	temperature	275:285	arg1	°C					300:301	140 °C	296:301	140 °C	296:301	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	0	51	dep	Yield	49:53	arg1	the					45:47	the	45:47	the	45:47	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	2	52	theme	pressure	329:336	arg1	effects					258:264	the effects	254:264	the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries	254:435	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	7	53	theme	fatty	1125:1129	arg1	profiles					1136:1143	the fatty acid profiles	1121:1143	the fatty acid profiles of camel milk powders	1121:1165	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	9	54	theme	nutritional	1494:1504	arg1	properties					1506:1515	the nutritional properties	1490:1515	the nutritional properties of the milk	1490:1527	The spray drying process must be optimized in order to retain the nutritional properties of the milk and the techno-functional characteristics of the powder.					
30440084	3	55	used	used	478:481	arg2	analysis					459:466	Principal components analysis	438:466	Principal components analysis (PCA)	438:472	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	3	55	used	used	478:481	arg2	PCA					469:471	PCA	469:471	PCA	469:471	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	9	56	theme	spray	1432:1436	arg1	process					1445:1451	The spray drying process	1428:1451	The spray drying process	1428:1451	The spray drying process must be optimized in order to retain the nutritional properties of the milk and the techno-functional characteristics of the powder.					
30440084	5	57	theme	flow	902:905	arg1	rate					907:910	feed flow rate	897:910	feed flow rate	897:910	The powder yield (cyclone recovery) increased with increasing inlet temperature and decreasing feed flow rate.					
30440084	7	58	theme	pressure	1210:1217	arg1	powders					1227:1233	high pressure and low pressure treated powders	1188:1233	high pressure and low pressure treated powders	1188:1233	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	7	59	theme	milk	1012:1015	arg1	powders					1017:1023	the camel milk powders	1002:1023	the camel milk powders	1002:1023	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	9	60	theme	milk	1524:1527	arg1	properties					1506:1515	the nutritional properties	1490:1515	the nutritional properties of the milk	1490:1527	The spray drying process must be optimized in order to retain the nutritional properties of the milk and the techno-functional characteristics of the powder.					
30440084	9	60	theme	milk	1524:1527	arg1	characteristics					1555:1569	the techno-functional characteristics	1533:1569	the techno-functional characteristics of the powder	1533:1583	The spray drying process must be optimized in order to retain the nutritional properties of the milk and the techno-functional characteristics of the powder.					
30440084	2	61	theme	temperature	275:285	arg1	effects					258:264	the effects	254:264	the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries	254:435	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	1	62	theme	powder	179:184	arg1	composition					153:163	the composition	149:163	the composition of camel milk powder	149:184	The effect of spray drying on the composition of camel milk powder was evaluated.					
30440084	10	63	theme	powder	1695:1700	arg1	components					1672:1681	selected nutritional components	1651:1681	selected nutritional components	1651:1681	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	10	63	theme	powder	1695:1700	arg1	yield					1641:1645	yield	1641:1645	yield	1641:1645	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	10	64	theme	bench	1722:1726	arg1	dryer					1738:1742	a bench top spray dryer	1720:1742	a bench top spray dryer	1720:1742	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	7	65	theme	high	1188:1191	arg1	pressure					1193:1200	high pressure	1188:1200	high pressure	1188:1200	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	7	66	theme	PCA	995:997	arg1	they					1026:1029	they	1026:1029	they	1026:1029	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	7	66	theme	PCA	995:997	arg1	result					985:990	a result	983:990	a result of PCA of the camel milk powders	983:1023	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	3	67	theme	whole	522:526	arg1	powders					539:545	spray dried whole camel milk powders	510:545	spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles	510:652	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	8	68	theme	milk	1375:1378	arg1	Drying					1359:1364	Drying	1359:1364	Drying of camel milk (using spray drying)	1359:1399	PRACTICAL APPLICATION: Drying of camel milk (using spray drying) can extend its shelf life.					
30440084	12	69	theme	important	1916:1924	arg1	components					1938:1947	important nutritional components	1916:1947	important nutritional components	1916:1947	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	2	70	theme	C	424:424	arg1	recoveries					426:435	vitamin C recoveries	416:435	vitamin C recoveries	416:435	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	2	71	used	used	242:245	arg2	design					231:236	A full factorial experimental design	201:236	A full factorial experimental design	201:236	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	3	72	theme	spray	510:514	arg1	powders					539:545	spray dried whole camel milk powders	510:545	spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles	510:652	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	7	73	theme	powders	1159:1165	arg1	profiles					1136:1143	the fatty acid profiles	1121:1143	the fatty acid profiles of camel milk powders	1121:1165	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	6	74	theme	outlet	918:923	arg1	temperature					925:935	High outlet temperature	913:935	High outlet temperature	913:935	High outlet temperature and pressure decreased vitamin C recovery.					
30440084	8	75	theme	PRACTICAL	1336:1344	arg1	APPLICATION					1346:1356	PRACTICAL APPLICATION	1336:1356	PRACTICAL APPLICATION: Drying of camel milk (using spray drying) can extend its shelf life.	1336:1426	PRACTICAL APPLICATION: Drying of camel milk (using spray drying) can extend its shelf life.					
30440084	1	76	theme	spray	133:137	arg1	drying					139:144	spray drying	133:144	spray drying	133:144	The effect of spray drying on the composition of camel milk powder was evaluated.					
30440084	7	77	theme	camel	1148:1152	arg1	powders					1159:1165	camel milk powders	1148:1165	camel milk powders	1148:1165	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	7	78	theme	acid	1131:1134	arg1	profiles					1136:1143	the fatty acid profiles	1121:1143	the fatty acid profiles of camel milk powders	1121:1165	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	3	79	theme	milk	534:537	arg1	powders					539:545	spray dried whole camel milk powders	510:545	spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles	510:652	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	4	80	theme	average	659:665	arg1	%					760:760	3.0%	757:760	3.0%	757:760	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	4	80	theme	average	659:665	arg1	composition					677:687	The average proximate composition	655:687	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid	655:750	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	0	81	theme	Camel	100:104	arg1	Powder					111:116	Whole Camel Milk Powder	94:116	Whole Camel Milk Powder	94:116	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	7	82	theme	acids	1329:1333	arg1	content					1312:1318	the content	1308:1318	the content of fatty acids	1308:1333	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	12	83	theme	processing	1978:1987	arg1	part					1965:1968	an important part	1952:1968	an important part of food processing	1952:1987	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	12	83	theme	processing	1978:1987	arg1	retention					1903:1911	retention	1903:1911	retention of important nutritional components	1903:1947	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	10	84	theme	drying	1616:1621	arg1	conditions					1623:1632	the spray drying conditions	1606:1632	the spray drying conditions	1606:1632	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	0	85	theme	Powder	111:116	arg1	Yield					49:53	Yield	49:53	Yield	49:53	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	0	85	theme	Powder	111:116	arg1	Components					80:89	Selected Nutritional Components	59:89	Selected Nutritional Components	59:89	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	1	86	theme	camel	168:172	arg1	powder					179:184	camel milk powder	168:184	camel milk powder	168:184	The effect of spray drying on the composition of camel milk powder was evaluated.					
30440084	10	87	theme	spray	1732:1736	arg1	dryer					1738:1742	a bench top spray dryer	1720:1742	a bench top spray dryer	1720:1742	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	10	88	theme	selected	1651:1658	arg1	components					1672:1681	selected nutritional components	1651:1681	selected nutritional components	1651:1681	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	5	89	theme	cyclone	820:826	arg1	yield					813:817	The powder yield	802:817	The powder yield (cyclone recovery)	802:836	The powder yield (cyclone recovery) increased with increasing inlet temperature and decreasing feed flow rate.					
30440084	5	89	theme	cyclone	820:826	arg1	recovery					828:835	cyclone recovery	820:835	cyclone recovery	820:835	The powder yield (cyclone recovery) increased with increasing inlet temperature and decreasing feed flow rate.					
30440084	4	90	theme	milk	702:705	arg1	viz.					707:710	the camel milk viz.	692:710	the camel milk viz.	692:710	The average proximate composition of the camel milk viz., fat, protein, lactose, and total solid were 3.0%, 3.21%, 3.01%, and 10.29% respectively.					
30440084	12	91	theme	important	1955:1963	arg1	part					1965:1968	an important part	1952:1968	an important part of food processing	1952:1987	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	12	91	theme	important	1955:1963	arg1	retention					1903:1911	retention	1903:1911	retention of important nutritional components	1903:1947	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	7	92	theme	milk	1091:1094	arg1	powders					1096:1102	high heat treated and low heat treated milk powders	1052:1102	high heat treated and low heat treated milk powders	1052:1102	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	3	93	theme	multiple	588:595	arg1	MCA					622:624	MCA	622:624	MCA	622:624	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	3	93	theme	multiple	588:595	arg1	analysis					612:619	multiple correspondence analysis	588:619	multiple correspondence analysis (MCA)	588:625	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	3	94	theme	components	448:457	arg1	PCA					469:471	PCA	469:471	PCA	469:471	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	3	94	theme	components	448:457	arg1	analysis					459:466	Principal components analysis	438:466	Principal components analysis (PCA)	438:472	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	7	95	theme	heat	1078:1081	arg1	powders					1096:1102	high heat treated and low heat treated milk powders	1052:1102	high heat treated and low heat treated milk powders	1052:1102	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	2	96	theme	full	203:206	arg1	design					231:236	A full factorial experimental design	201:236	A full factorial experimental design	201:236	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	2	97	theme	feed	366:369	arg1	rev/s					394:398	5, 4, and 3 rev/s	382:398	5, 4, and 3 rev/s	382:398	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	2	97	theme	feed	366:369	arg1	rate					376:379	feed flow rate	366:379	feed flow rate (5, 4, and 3 rev/s)	366:399	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	12	98	theme	nutritional	1926:1936	arg1	components					1938:1947	important nutritional components	1916:1947	important nutritional components	1916:1947	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	5	99	theme	inlet	864:868	arg1	temperature					870:880	inlet temperature	864:880	inlet temperature	864:880	The powder yield (cyclone recovery) increased with increasing inlet temperature and decreasing feed flow rate.					
30440084	7	100	theme	higher	1261:1266	arg1	pressure					1280:1287	higher atomization pressure	1261:1287	higher atomization pressure	1261:1287	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	0	101	from	Effect	4:9	arg1	Yield					49:53	Yield	49:53	Yield	49:53	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	0	101	from	Effect	4:9	arg1	Components					80:89	Selected Nutritional Components	59:89	Selected Nutritional Components	59:89	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	0	102	theme	Nutritional	68:78	arg1	Components					80:89	Selected Nutritional Components	59:89	Selected Nutritional Components	59:89	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	2	103	from	effects	258:264	arg1	recoveries					426:435	vitamin C recoveries	416:435	vitamin C recoveries	416:435	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	2	103	from	effects	258:264	arg1	cyclone					404:410	cyclone	404:410	cyclone	404:410	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	3	104	theme	fatty	634:638	arg1	profiles					645:652	the fatty acid profiles	630:652	the fatty acid profiles	630:652	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	2	105	theme	rate	376:379	arg1	effects					258:264	the effects	254:264	the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries	254:435	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	12	106	theme	spray	1873:1877	arg1	operations					1886:1895	future spray drying operations	1866:1895	future spray drying operations	1866:1895	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	0	107	theme	Pretreatment	14:25	arg1	Effect					4:9	The Effect	0:9	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.	0:117	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	11	108	theme	industrial	1802:1811	arg1	dryer					1820:1824	an industrial volume dryer	1799:1824	an industrial volume dryer	1799:1824	However, there is need to translate these findings to an industrial volume dryer.					
30440084	12	109	theme	big	1854:1856	arg1	role					1858:1861	a big role	1852:1861	a big role	1852:1861	These findings will play a big role in future spray drying operations since retention of important nutritional components is an important part of food processing.					
30440084	2	110	theme	atomization	317:327	arg1	bar					357:359	800, 600, and 400 bar	339:359	800, 600, and 400 bar	339:359	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	2	110	theme	atomization	317:327	arg1	pressure					329:336	atomization pressure	317:336	atomization pressure (800, 600, and 400 bar)	317:360	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	0	111	theme	Spray	28:32	arg1	Pretreatment					14:25	Pretreatment	14:25	Pretreatment (Spray Drying)	14:40	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	0	111	theme	Spray	28:32	arg1	Drying					34:39	Spray Drying	28:39	Spray Drying	28:39	The Effect of Pretreatment (Spray Drying) on the Yield and Selected Nutritional Components of Whole Camel Milk Powder.					
30440084	7	112	theme	powders	1017:1023	arg1	PCA					995:997	PCA	995:997	PCA of the camel milk powders	995:1023	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	10	113	from	effect	1596:1601	arg1	components					1672:1681	selected nutritional components	1651:1681	selected nutritional components	1651:1681	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	10	113	from	effect	1596:1601	arg1	yield					1641:1645	yield	1641:1645	yield	1641:1645	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	5	114	theme	feed	897:900	arg1	rate					907:910	feed flow rate	897:910	feed flow rate	897:910	The powder yield (cyclone recovery) increased with increasing inlet temperature and decreasing feed flow rate.					
30440084	7	115	theme	camel	1006:1010	arg1	powders					1017:1023	the camel milk powders	1002:1023	the camel milk powders	1002:1023	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	7	116	from	increase	1296:1303	arg1	content					1312:1318	the content	1308:1318	the content of fatty acids	1308:1333	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	7	117	theme	treated	1219:1225	arg1	powders					1227:1233	high pressure and low pressure treated powders	1188:1233	high pressure and low pressure treated powders	1188:1233	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	10	118	theme	top	1728:1730	arg1	dryer					1738:1742	a bench top spray dryer	1720:1742	a bench top spray dryer	1720:1742	Here, the effect of the spray drying conditions on the yield and selected nutritional components of the milk powder was studied using a bench top spray dryer.					
30440084	2	119	theme	inlet	269:273	arg1	temperature					275:285	inlet temperature	269:285	inlet temperature (160 °C, 140 °C, and 120 °C)	269:314	A full factorial experimental design was used to see the effects of inlet temperature (160 °C, 140 °C, and 120 °C), atomization pressure (800, 600, and 400 bar) and feed flow rate (5, 4, and 3 rev/s) on cyclone and vitamin C recoveries.					
30440084	8	120	theme	camel	1369:1373	arg1	milk					1375:1378	camel milk	1369:1378	camel milk	1369:1378	PRACTICAL APPLICATION: Drying of camel milk (using spray drying) can extend its shelf life.					
30440084	7	121	theme	low	1206:1208	arg1	pressure					1210:1217	low pressure	1206:1217	low pressure	1206:1217	As a result of PCA of the camel milk powders, they were classified into high heat treated and low heat treated milk powders while the MCA of the fatty acid profiles of camel milk powders were classified into high pressure and low pressure treated powders and it was concluded that higher atomization pressure caused increase in the content of fatty acids.					
30440084	9	122	theme	techno-functional	1537:1553	arg1	characteristics					1555:1569	the techno-functional characteristics	1533:1569	the techno-functional characteristics of the powder	1533:1583	The spray drying process must be optimized in order to retain the nutritional properties of the milk and the techno-functional characteristics of the powder.					
30440084	3	123	theme	dried	516:520	arg1	powders					539:545	spray dried whole camel milk powders	510:545	spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles	510:652	Principal components analysis (PCA) was used to check classification of spray dried whole camel milk powders based on their processing conditions and multiple correspondence analysis (MCA) on the fatty acid profiles.					
30440084	6	124	theme	vitamin	960:966	arg1	recovery					970:977	vitamin C recovery	960:977	vitamin C recovery	960:977	High outlet temperature and pressure decreased vitamin C recovery.					
31888837	5	0	theme	dextran-based	858:870	arg1	sensitive					889:897	sensitive	889:897	sensitive	889:897	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	5	0	theme	dextran-based	858:870	arg1	coacervates					872:882	the dextran-based coacervates	854:882	the dextran-based coacervates	854:882	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	2	1	from	backbones	452:460	arg1	copolymerization					401:416	the copolymerization	397:416	the copolymerization of cationic monomers from dextran backbones	397:460	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	2	1	from	backbones	452:460	arg1	monomers					430:437	cationic monomers	421:437	cationic monomers from dextran backbones	421:460	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	1	2	theme	gene	171:174	arg1	transport					176:184	gene transport	171:184	gene transport	171:184	The intractable toxicity of cationic polymers limits their applicability in gene transport and controlled release.					
31888837	6	3	used	used	1090:1093	arg2	coacervates					1071:1081	dextran-based coacervates	1057:1081	dextran-based coacervates	1057:1081	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	5	4	theme	in	821:822	arg1	experiments					830:840	The in vitro experiments	817:840	The in vitro experiments	817:840	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	4	5	theme	disulfide	733:741	arg1	points					750:755	redox-responsive disulfide branch points	716:755	redox-responsive disulfide branch points	716:755	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	6	6	theme	dextran-based	1057:1069	arg1	coacervates					1071:1081	dextran-based coacervates	1057:1081	dextran-based coacervates	1057:1081	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	6	7	contain	carry	1098:1102	arg1	coacervates					1071:1081	dextran-based coacervates	1057:1081	dextran-based coacervates	1057:1081	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	6	7	contain	carry	1098:1102	arg2	siRNA					1104:1108	siRNA	1104:1108	siRNA into cancer cells with a high transfection efficiency	1104:1162	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	4	8	theme	reducing	780:787	arg1	responsiveness					801:814	reducing environment responsiveness	780:814	reducing environment responsiveness	780:814	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	5	9	theme	genes	1023:1027	arg1	uptake					991:996	uptake	991:996	uptake	991:996	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	5	9	theme	genes	1023:1027	arg1	transfection					1003:1014	transfection	1003:1014	transfection	1003:1014	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	5	9	theme	genes	1023:1027	arg1	release					982:988	an effective release	969:988	an effective release	969:988	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	6	10	theme	transfection	1140:1151	arg1	efficiency					1153:1162	a high transfection efficiency	1133:1162	a high transfection efficiency	1133:1162	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	2	11	theme	monomers	430:437	arg1	copolymerization					401:416	the copolymerization	397:416	the copolymerization of cationic monomers from dextran backbones	397:460	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	4	12	with	coacervates	763:773	arg1	responsiveness					801:814	reducing environment responsiveness	780:814	reducing environment responsiveness	780:814	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	1	13	theme	controlled	190:199	arg1	release					201:207	controlled release	190:207	controlled release	190:207	The intractable toxicity of cationic polymers limits their applicability in gene transport and controlled release.					
31888837	5	14	theme	reducing	906:913	arg1	environment					915:925	the reducing environment	902:925	the reducing environment	902:925	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	4	15	theme	redox-responsive	716:731	arg1	points					750:755	redox-responsive disulfide branch points	716:755	redox-responsive disulfide branch points	716:755	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	2	16	theme	amino	334:338	arg1	copolymers					366:375	amino group-containing cationic copolymers	334:375	amino group-containing cationic copolymers based on dextran	334:392	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	5	17	theme	effective	972:980	arg1	release					982:988	an effective release	969:988	an effective release	969:988	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	4	18	theme	resultant	614:622	arg1	coacervates					624:634	The resultant coacervates	610:634	The resultant coacervates	610:634	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	1	19	theme	intractable	99:109	arg1	toxicity					111:118	The intractable toxicity	95:118	The intractable toxicity of cationic polymers	95:139	The intractable toxicity of cationic polymers limits their applicability in gene transport and controlled release.					
31888837	4	20	theme	branch	743:748	arg1	points					750:755	redox-responsive disulfide branch points	716:755	redox-responsive disulfide branch points	716:755	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	6	21	with	siRNA	1104:1108	arg1	efficiency					1153:1162	a high transfection efficiency	1133:1162	a high transfection efficiency	1133:1162	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	4	22	theme	genes	705:709	arg1	self-assembly					657:669	the self-assembly	653:669	the self-assembly of cationic coplymers and anionic genes	653:709	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	5	23	theme	293T	1032:1035	arg1	cells					1037:1041	293T cells	1032:1041	293T cells	1032:1041	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	4	24	theme	anionic	697:703	arg1	genes					705:709	anionic genes	697:709	anionic genes	697:709	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	0	25	theme	gene	51:54	arg1	nanodroplets					25:36	nanodroplets	25:36	nanodroplets	25:36	Dextran-based coacervate nanodroplets as potential gene carriers for efficient cancer therapy.					
31888837	0	25	theme	gene	51:54	arg1	carriers					56:63	potential gene carriers	41:63	potential gene carriers for efficient cancer therapy	41:92	Dextran-based coacervate nanodroplets as potential gene carriers for efficient cancer therapy.					
31888837	2	26	theme	copolymers	366:375	arg1	types					325:329	two types	321:329	two types of amino group-containing cationic copolymers based on dextran	321:392	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	3	27	contain	containing	496:505	arg1	crosslinkers					483:494	allyl crosslinkers	477:494	allyl crosslinkers containing disulfide bonds	477:521	Additionally, allyl crosslinkers containing disulfide bonds were introduced into polymerization, that made the copolymer crosslinked by disulfide.					
31888837	3	27	contain	containing	496:505	arg2	bonds					517:521	disulfide bonds	507:521	disulfide bonds	507:521	Additionally, allyl crosslinkers containing disulfide bonds were introduced into polymerization, that made the copolymer crosslinked by disulfide.					
31888837	2	28	theme	biofunctionality	260:275	arg1	consideration					213:225	consideration	213:225	consideration of the good biocompatibility and biofunctionality of dextran	213:286	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	6	29	theme	high	1135:1138	arg1	efficiency					1153:1162	a high transfection efficiency	1133:1162	a high transfection efficiency	1133:1162	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	2	30	theme	dextran	444:450	arg1	backbones					452:460	dextran backbones	444:460	dextran backbones	444:460	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	0	31	theme	potential	41:49	arg1	nanodroplets					25:36	nanodroplets	25:36	nanodroplets	25:36	Dextran-based coacervate nanodroplets as potential gene carriers for efficient cancer therapy.					
31888837	0	31	theme	potential	41:49	arg1	carriers					56:63	potential gene carriers	41:63	potential gene carriers for efficient cancer therapy	41:92	Dextran-based coacervate nanodroplets as potential gene carriers for efficient cancer therapy.					
31888837	3	32	theme	disulfide	507:515	arg1	bonds					517:521	disulfide bonds	507:521	disulfide bonds	507:521	Additionally, allyl crosslinkers containing disulfide bonds were introduced into polymerization, that made the copolymer crosslinked by disulfide.					
31888837	3	33	theme	allyl	477:481	arg1	crosslinkers					483:494	allyl crosslinkers	477:494	allyl crosslinkers containing disulfide bonds	477:521	Additionally, allyl crosslinkers containing disulfide bonds were introduced into polymerization, that made the copolymer crosslinked by disulfide.					
31888837	2	34	theme	biocompatibility	239:254	arg1	consideration					213:225	consideration	213:225	consideration of the good biocompatibility and biofunctionality of dextran	213:286	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	2	35	from	copolymerization	401:416	arg1	backbones					452:460	dextran backbones	444:460	dextran backbones	444:460	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	1	36	theme	cationic	123:130	arg1	polymers					132:139	cationic polymers	123:139	cationic polymers	123:139	The intractable toxicity of cationic polymers limits their applicability in gene transport and controlled release.					
31888837	2	37	theme	good	234:237	arg1	biocompatibility					239:254	the good biocompatibility	230:254	the good biocompatibility	230:254	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	1	38	theme	polymers	132:139	arg1	toxicity					111:118	The intractable toxicity	95:118	The intractable toxicity of cationic polymers	95:139	The intractable toxicity of cationic polymers limits their applicability in gene transport and controlled release.					
31888837	5	39	dep	in	821:822	arg1	vitro					824:828	vitro	824:828	vitro	824:828	The in vitro experiments showed that the dextran-based coacervates were sensitive to the reducing environment and underwent cleavage, which resulted in an effective release, uptake, and transfection of the genes by 293T cells.					
31888837	2	40	theme	cationic	357:364	arg1	copolymers					366:375	amino group-containing cationic copolymers	334:375	amino group-containing cationic copolymers based on dextran	334:392	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	4	41	theme	cationic	674:681	arg1	coplymers					683:691	cationic coplymers	674:691	cationic coplymers	674:691	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	2	42	theme	group-containing	340:355	arg1	copolymers					366:375	amino group-containing cationic copolymers	334:375	amino group-containing cationic copolymers based on dextran	334:392	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	0	43	theme	cancer	79:84	arg1	therapy					86:92	efficient cancer therapy	69:92	efficient cancer therapy	69:92	Dextran-based coacervate nanodroplets as potential gene carriers for efficient cancer therapy.					
31888837	6	44	theme	cancer	1115:1120	arg1	cells					1122:1126	cancer cells	1115:1126	cancer cells	1115:1126	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	2	45	theme	dextran	280:286	arg1	biofunctionality					260:275	biofunctionality	260:275	biofunctionality of dextran	260:286	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	2	45	theme	dextran	280:286	arg1	biocompatibility					239:254	the good biocompatibility	230:254	the good biocompatibility	230:254	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	2	46	theme	cationic	421:428	arg1	monomers					430:437	cationic monomers	421:437	cationic monomers from dextran backbones	421:460	In consideration of the good biocompatibility and biofunctionality of dextran, herein we design and synthesize two types of amino group-containing cationic copolymers based on dextran by the copolymerization of cationic monomers from dextran backbones.					
31888837	0	47	theme	efficient	69:77	arg1	therapy					86:92	efficient cancer therapy	69:92	efficient cancer therapy	69:92	Dextran-based coacervate nanodroplets as potential gene carriers for efficient cancer therapy.					
31888837	4	48	theme	coplymers	683:691	arg1	self-assembly					657:669	the self-assembly	653:669	the self-assembly of cationic coplymers and anionic genes	653:709	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
31888837	6	49	from	applicability	1195:1207	arg1	treatment					1212:1220	treatment	1212:1220	treatment	1212:1220	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	6	50	theme	potential	1185:1193	arg1	applicability					1195:1207	their potential applicability	1179:1207	their potential applicability in treatment against cancer	1179:1235	In addition, dextran-based coacervates can be used to carry siRNA into cancer cells with a high transfection efficiency, demonstrating their potential applicability in treatment against cancer.					
31888837	4	51	theme	environment	789:799	arg1	responsiveness					801:814	reducing environment responsiveness	780:814	reducing environment responsiveness	780:814	The resultant coacervates were formed from the self-assembly of cationic coplymers and anionic genes, and redox-responsive disulfide branch points endow coacervates with reducing environment responsiveness.					
30516277	11	0	theme	shelf	1983:1987	arg1	life					1989:1992	shelf life	1983:1992	shelf life of products	1983:2004	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	11	1	theme	food	1959:1962	arg1	quality					1964:1970	guarantee food quality	1949:1970	guarantee food quality	1949:1970	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	8	2	theme	in	1522:1523	arg1	coatings					1544:1551	the in situ SiOx chitosan coatings	1518:1551	the in situ SiOx chitosan coatings	1518:1551	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	10	3	theme	due	1770:1772	arg1	material					1761:1768	a potential preservation material	1736:1768	a potential preservation material due to the increasing mechanical preservation properties	1736:1825	It could serve as a potential preservation material due to the increasing mechanical preservation properties.					
30516277	10	3	theme	due	1770:1772	arg1	It					1718:1719	It	1718:1719	It	1718:1719	It could serve as a potential preservation material due to the increasing mechanical preservation properties.					
30516277	1	4	theme	ZnO/TiO2	406:413	arg1	nano-particles					417:430	zinc oxide/titania (ZnO/TiO2 ) nano-particles	386:430	zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs)	386:436	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	1	4	theme	ZnO/TiO2	406:413	arg1	NPs					433:435	NPs	433:435	NPs	433:435	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	1	5	theme	fish	248:251	arg1	balls					253:257	silver carp fish balls	236:257	silver carp fish balls	236:257	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	7	6	theme	shelf	1379:1383	arg1	time					1385:1388	shelf time	1379:1388	shelf time of Sciaenops ocellatus	1379:1411	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	4	7	theme	freshness	794:802	arg1	index					804:808	the freshness index	790:808	the freshness index	790:808	The change of the freshness index and the texture of the fish balls are delayed by the coatings due to their gas permeability and antibacterial properties.					
30516277	8	8	theme	SiOx	1530:1533	arg1	coatings					1544:1551	the in situ SiOx chitosan coatings	1518:1551	the in situ SiOx chitosan coatings	1518:1551	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	6	9	theme	preservation	1166:1177	arg1	properties					1179:1188	the preservation properties	1162:1188	the preservation properties of the chitosan coating	1162:1212	Furthermore, the surface modification of ZnO/TiO2 NPs enhances the preservation properties of the chitosan coating.					
30516277	7	10	theme	preservation	1313:1324	arg1	properties					1326:1335	antibacterial and preservation properties	1295:1335	antibacterial and preservation properties of chitosan coating	1295:1355	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	10	11	theme	mechanical	1792:1801	arg1	properties					1816:1825	the increasing mechanical preservation properties	1777:1825	the increasing mechanical preservation properties	1777:1825	It could serve as a potential preservation material due to the increasing mechanical preservation properties.					
30516277	5	12	theme	NPs	1094:1096	arg1	co-modification					1066:1080	co-modification	1066:1080	co-modification of ZnO/TiO2 NPs	1066:1096	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	11	13	theme	ZTS-CS	1878:1883	arg1	coatings					1886:1893	the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings	1849:1893	the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings	1849:1893	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	7	14	theme	antibacterial	1295:1307	arg1	properties					1326:1335	antibacterial and preservation properties	1295:1335	antibacterial and preservation properties of chitosan coating	1295:1355	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	7	15	located	found	1278:1282	arg2	SiOx					1269:1272	in situ SiOx	1261:1272	in situ SiOx	1261:1272	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	7	15	located	found	1278:1282	arg1	study					1254:1258	our previous study	1241:1258	our previous study	1241:1258	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	6	16	theme	ZnO/TiO2	1140:1147	arg1	NPs					1149:1151	ZnO/TiO2 NPs	1140:1151	ZnO/TiO2 NPs	1140:1151	Furthermore, the surface modification of ZnO/TiO2 NPs enhances the preservation properties of the chitosan coating.					
30516277	2	17	theme	chemical	504:511	arg1	bonds					513:517	chemical bonds	504:517	chemical bonds	504:517	In the chitosan coatings, when the chitosan combines with NPs by chemical bonds, the crystal lattice is slightly changed due to the modification of NPs.					
30516277	0	18	theme	Silver	102:107	arg1	Carp					109:112	Silver Carp	102:112	Silver Carp Fish Balls	102:123	The Modification of In Situ SiOx Chitosan Coatings by ZnO/TiO2 NPs and Its Preservation Properties to Silver Carp Fish Balls.					
30516277	5	19	theme	coatings	976:983	arg1	properties					949:958	The preservation properties	932:958	The preservation properties of the chitosan coatings for Silver Carp fish balls	932:1010	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	4	20	theme	balls	838:842	arg1	texture					818:824	the texture	814:824	the texture of the fish balls	814:842	The change of the freshness index and the texture of the fish balls are delayed by the coatings due to their gas permeability and antibacterial properties.					
30516277	4	20	theme	balls	838:842	arg1	change					780:785	The change	776:785	The change of the freshness index	776:808	The change of the freshness index and the texture of the fish balls are delayed by the coatings due to their gas permeability and antibacterial properties.					
30516277	3	21	theme	/SiOx-chitosan	711:724	arg1	coating					735:741	sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating	661:741	sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating	661:741	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	3	21	theme	/SiOx-chitosan	711:724	arg1	one					771:773	one	771:773	one	771:773	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	0	22	theme	Fish	114:117	arg1	Balls					119:123	Silver Carp Fish Balls	102:123	Silver Carp Fish Balls	102:123	The Modification of In Situ SiOx Chitosan Coatings by ZnO/TiO2 NPs and Its Preservation Properties to Silver Carp Fish Balls.					
30516277	5	23	theme	Silver	989:994	arg1	Carp					996:999	Silver Carp	989:999	Silver Carp fish balls	989:1010	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	7	24	dep	in	1261:1262	arg1	situ					1264:1267	situ	1264:1267	situ	1264:1267	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	2	25	theme	crystal	524:530	arg1	lattice					532:538	the crystal lattice	520:538	the crystal lattice	520:538	In the chitosan coatings, when the chitosan combines with NPs by chemical bonds, the crystal lattice is slightly changed due to the modification of NPs.					
30516277	6	26	theme	surface	1116:1122	arg1	modification					1124:1135	the surface modification	1112:1135	the surface modification of ZnO/TiO2 NPs	1112:1151	Furthermore, the surface modification of ZnO/TiO2 NPs enhances the preservation properties of the chitosan coating.					
30516277	3	27	theme	modified	693:700	arg1	coating					735:741	sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating	661:741	sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating	661:741	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	3	27	theme	modified	693:700	arg1	one					771:773	one	771:773	one	771:773	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	6	28	theme	chitosan	1197:1204	arg1	coating					1206:1212	the chitosan coating	1193:1212	the chitosan coating	1193:1212	Furthermore, the surface modification of ZnO/TiO2 NPs enhances the preservation properties of the chitosan coating.					
30516277	1	29	theme	oxide/titania	391:403	arg1	nano-particles					417:430	zinc oxide/titania (ZnO/TiO2 ) nano-particles	386:430	zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs)	386:436	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	1	29	theme	oxide/titania	391:403	arg1	NPs					433:435	NPs	433:435	NPs	433:435	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	11	30	theme	/SiOx-chitosan	1862:1875	arg1	coatings					1886:1893	the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings	1849:1893	the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings	1849:1893	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	4	31	theme	due	872:874	arg1	coatings					863:870	the coatings	859:870	the coatings due to their gas permeability and antibacterial properties	859:929	The change of the freshness index and the texture of the fish balls are delayed by the coatings due to their gas permeability and antibacterial properties.					
30516277	7	32	theme	previous	1245:1252	arg1	study					1254:1258	our previous study	1241:1258	our previous study	1241:1258	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	3	33	theme	ZTS-CS	727:732	arg1	coating					735:741	sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating	661:741	sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating	661:741	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	3	33	theme	ZTS-CS	727:732	arg1	one					771:773	one	771:773	one	771:773	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	1	34	theme	coatings	322:329	arg1	microstructures					268:282	microstructures	268:282	microstructures	268:282	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	1	34	theme	coatings	322:329	arg1	properties					304:313	physicochemical properties	288:313	physicochemical properties	288:313	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	2	35	theme	chitosan	446:453	arg1	coatings					455:462	the chitosan coatings	442:462	the chitosan coatings	442:462	In the chitosan coatings, when the chitosan combines with NPs by chemical bonds, the crystal lattice is slightly changed due to the modification of NPs.					
30516277	8	36	dep	in	1522:1523	arg1	situ					1525:1528	situ	1525:1528	situ	1525:1528	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	1	37	theme	chitosan	140:147	arg1	coatings					149:156	The composite chitosan coatings	126:156	The composite chitosan coatings	126:156	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	5	38	theme	fish	1001:1004	arg1	balls					1006:1010	Silver Carp fish balls	989:1010	Silver Carp fish balls	989:1010	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	6	39	mod	modification	1124:1135	arg1	NPs					1149:1151	ZnO/TiO2 NPs	1140:1151	ZnO/TiO2 NPs	1140:1151	Furthermore, the surface modification of ZnO/TiO2 NPs enhances the preservation properties of the chitosan coating.					
30516277	6	39	mod	modification	1124:1135	arg3	surface					1116:1122	the surface modification	1112:1135	the surface modification of ZnO/TiO2 NPs	1112:1151	Furthermore, the surface modification of ZnO/TiO2 NPs enhances the preservation properties of the chitosan coating.					
30516277	1	40	theme	nano	356:359	arg1	SiOx					376:379	SiOx	376:379	SiOx	376:379	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	1	40	theme	nano	356:359	arg1	oxide					369:373	in situ nano silicon oxide	348:373	in situ nano silicon oxide (SiOx)	348:380	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	5	41	dep	in	1028:1029	arg1	situ					1031:1034	situ	1031:1034	situ	1031:1034	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	9	42	dep	In	1554:1555	arg1	situ					1557:1560	situ	1557:1560	situ	1557:1560	In situ SiOx modified by ZnO/TiO2 NPs were synthesized, measured, and characterized in this study, and were applied for the preservation of silver carp fish balls.					
30516277	7	43	theme	PRACTICAL	1215:1223	arg1	APPLICATION					1225:1235	PRACTICAL APPLICATION	1215:1235	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.	1215:1412	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	9	44	theme	silver	1694:1699	arg1	balls					1711:1715	silver carp fish balls	1694:1715	silver carp fish balls	1694:1715	In situ SiOx modified by ZnO/TiO2 NPs were synthesized, measured, and characterized in this study, and were applied for the preservation of silver carp fish balls.					
30516277	8	45	theme	chitosan	1456:1463	arg1	coatings					1465:1472	chitosan coatings	1456:1472	chitosan coatings	1456:1472	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	1	46	theme	physicochemical	288:302	arg1	properties					304:313	physicochemical properties	288:313	physicochemical properties	288:313	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	4	47	theme	gas	885:887	arg1	permeability					889:900	their gas permeability	879:900	their gas permeability	879:900	The change of the freshness index and the texture of the fish balls are delayed by the coatings due to their gas permeability and antibacterial properties.					
30516277	9	48	theme	fish	1706:1709	arg1	balls					1711:1715	silver carp fish balls	1694:1715	silver carp fish balls	1694:1715	In situ SiOx modified by ZnO/TiO2 NPs were synthesized, measured, and characterized in this study, and were applied for the preservation of silver carp fish balls.					
30516277	0	49	theme	Chitosan	33:40	arg1	Modification					4:15	The Modification	0:15	The Modification of In Situ SiOx Chitosan	0:40	The Modification of In Situ SiOx Chitosan Coatings by ZnO/TiO2 NPs and Its Preservation Properties to Silver Carp Fish Balls.					
30516277	5	50	theme	preservation	936:947	arg1	properties					949:958	The preservation properties	932:958	The preservation properties of the chitosan coatings for Silver Carp fish balls	932:1010	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	10	51	theme	potential	1738:1746	arg1	material					1761:1768	a potential preservation material	1736:1768	a potential preservation material due to the increasing mechanical preservation properties	1736:1825	It could serve as a potential preservation material due to the increasing mechanical preservation properties.					
30516277	10	51	theme	potential	1738:1746	arg1	It					1718:1719	It	1718:1719	It	1718:1719	It could serve as a potential preservation material due to the increasing mechanical preservation properties.					
30516277	8	52	theme	chitosan	1535:1542	arg1	coatings					1544:1551	the in situ SiOx chitosan coatings	1518:1551	the in situ SiOx chitosan coatings	1518:1551	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	1	53	theme	preservation	208:219	arg1	properties					221:230	their preservation properties	202:230	their preservation properties	202:230	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	7	54	theme	Sciaenops	1393:1401	arg1	ocellatus					1403:1411	Sciaenops ocellatus	1393:1411	Sciaenops ocellatus	1393:1411	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	8	55	theme	edible	1493:1498	arg1	materials					1505:1513	nontoxic edible nano materials	1484:1513	nontoxic edible nano materials	1484:1513	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	4	56	theme	antibacterial	906:918	arg1	properties					920:929	antibacterial properties	906:929	antibacterial properties	906:929	The change of the freshness index and the texture of the fish balls are delayed by the coatings due to their gas permeability and antibacterial properties.					
30516277	11	57	theme	food	1932:1935	arg1	industry					1937:1944	the food industry	1928:1944	the food industry	1928:1944	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	1	58	theme	carp	243:246	arg1	balls					253:257	silver carp fish balls	236:257	silver carp fish balls	236:257	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	9	59	theme	In	1554:1555	arg1	SiOx					1562:1565	In situ SiOx	1554:1565	In situ SiOx modified by ZnO/TiO2 NPs	1554:1590	In situ SiOx modified by ZnO/TiO2 NPs were synthesized, measured, and characterized in this study, and were applied for the preservation of silver carp fish balls.					
30516277	7	60	theme	chitosan	1340:1347	arg1	properties					1326:1335	antibacterial and preservation properties	1295:1335	antibacterial and preservation properties of chitosan coating	1295:1355	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	10	61	theme	preservation	1803:1814	arg1	properties					1816:1825	the increasing mechanical preservation properties	1777:1825	the increasing mechanical preservation properties	1777:1825	It could serve as a potential preservation material due to the increasing mechanical preservation properties.					
30516277	7	62	theme	coating	1349:1355	arg1	chitosan					1340:1347	chitosan coating	1340:1355	chitosan coating	1340:1355	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	1	63	dep	in	348:349	arg1	situ					351:354	situ	351:354	situ	351:354	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	10	64	theme	increasing	1781:1790	arg1	properties					1816:1825	the increasing mechanical preservation properties	1777:1825	the increasing mechanical preservation properties	1777:1825	It could serve as a potential preservation material due to the increasing mechanical preservation properties.					
30516277	6	65	theme	NPs	1149:1151	arg1	modification					1124:1135	the surface modification	1112:1135	the surface modification of ZnO/TiO2 NPs	1112:1151	Furthermore, the surface modification of ZnO/TiO2 NPs enhances the preservation properties of the chitosan coating.					
30516277	11	66	theme	products	1997:2004	arg1	life					1989:1992	shelf life	1983:1992	shelf life of products	1983:2004	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	0	67	theme	Carp	109:112	arg1	Balls					119:123	Silver Carp Fish Balls	102:123	Silver Carp Fish Balls	102:123	The Modification of In Situ SiOx Chitosan Coatings by ZnO/TiO2 NPs and Its Preservation Properties to Silver Carp Fish Balls.					
30516277	4	68	theme	fish	833:836	arg1	balls					838:842	the fish balls	829:842	the fish balls	829:842	The change of the freshness index and the texture of the fish balls are delayed by the coatings due to their gas permeability and antibacterial properties.					
30516277	5	69	theme	Carp	996:999	arg1	balls					1006:1010	Silver Carp fish balls	989:1010	Silver Carp fish balls	989:1010	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	4	70	theme	index	804:808	arg1	texture					818:824	the texture	814:824	the texture of the fish balls	814:842	The change of the freshness index and the texture of the fish balls are delayed by the coatings due to their gas permeability and antibacterial properties.					
30516277	4	70	theme	index	804:808	arg1	change					780:785	The change	776:785	The change of the freshness index	776:808	The change of the freshness index and the texture of the fish balls are delayed by the coatings due to their gas permeability and antibacterial properties.					
30516277	3	71	theme	ZnO/TiO2	702:709	arg1	coating					735:741	sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating	661:741	sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating	661:741	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	3	71	theme	ZnO/TiO2	702:709	arg1	one					771:773	one	771:773	one	771:773	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	6	72	theme	coating	1206:1212	arg1	properties					1179:1188	the preservation properties	1162:1188	the preservation properties of the chitosan coating	1162:1212	Furthermore, the surface modification of ZnO/TiO2 NPs enhances the preservation properties of the chitosan coating.					
30516277	1	73	theme	zinc	386:389	arg1	nano-particles					417:430	zinc oxide/titania (ZnO/TiO2 ) nano-particles	386:430	zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs)	386:436	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	1	73	theme	zinc	386:389	arg1	NPs					433:435	NPs	433:435	NPs	433:435	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	11	74	theme	ZnO/TiO2	1853:1860	arg1	coatings					1886:1893	the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings	1849:1893	the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings	1849:1893	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	11	75	theme	guarantee	1949:1957	arg1	quality					1964:1970	guarantee food quality	1949:1970	guarantee food quality	1949:1970	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	7	76	theme	in	1261:1262	arg1	SiOx					1269:1272	in situ SiOx	1261:1272	in situ SiOx	1261:1272	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	0	77	theme	ZnO/TiO2	54:61	arg1	NPs					63:65	ZnO/TiO2 NPs	54:65	ZnO/TiO2 NPs	54:65	The Modification of In Situ SiOx Chitosan Coatings by ZnO/TiO2 NPs and Its Preservation Properties to Silver Carp Fish Balls.					
30516277	5	78	theme	chitosan	967:974	arg1	coatings					976:983	the chitosan coatings	963:983	the chitosan coatings for Silver Carp fish balls	963:1010	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	1	79	dep	microstructures	268:282	arg1	the					264:266	the	264:266	the	264:266	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	8	80	dep	improve	1434:1440	arg1	to					1423:1424	to	1423:1424	to	1423:1424	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	5	81	theme	in	1028:1029	arg1	SiOx					1036:1039	in situ SiOx	1028:1039	in situ SiOx	1028:1039	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	1	82	theme	composite	130:138	arg1	coatings					149:156	The composite chitosan coatings	126:156	The composite chitosan coatings	126:156	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	1	83	theme	in	348:349	arg1	SiOx					376:379	SiOx	376:379	SiOx	376:379	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	1	83	theme	in	348:349	arg1	oxide					369:373	in situ nano silicon oxide	348:373	in situ nano silicon oxide (SiOx)	348:380	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	0	84	theme	Preservation	75:86	arg1	Properties					88:97	Its Preservation Properties	71:97	Its Preservation Properties	71:97	The Modification of In Situ SiOx Chitosan Coatings by ZnO/TiO2 NPs and Its Preservation Properties to Silver Carp Fish Balls.					
30516277	2	85	theme	NPs	587:589	arg1	modification					571:582	the modification	567:582	the modification of NPs	567:589	In the chitosan coatings, when the chitosan combines with NPs by chemical bonds, the crystal lattice is slightly changed due to the modification of NPs.					
30516277	1	86	theme	silicon	361:367	arg1	SiOx					376:379	SiOx	376:379	SiOx	376:379	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	1	86	theme	silicon	361:367	arg1	oxide					369:373	in situ nano silicon oxide	348:373	in situ nano silicon oxide (SiOx)	348:380	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
30516277	0	87	theme	In	20:21	arg1	Chitosan					33:40	In Situ SiOx Chitosan	20:40	In Situ SiOx Chitosan	20:40	The Modification of In Situ SiOx Chitosan Coatings by ZnO/TiO2 NPs and Its Preservation Properties to Silver Carp Fish Balls.					
30516277	8	88	theme	coatings	1465:1472	arg1	properties					1442:1451	properties	1442:1451	properties of chitosan coatings	1442:1472	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	0	89	theme	SiOx	28:31	arg1	Chitosan					33:40	In Situ SiOx Chitosan	20:40	In Situ SiOx Chitosan	20:40	The Modification of In Situ SiOx Chitosan Coatings by ZnO/TiO2 NPs and Its Preservation Properties to Silver Carp Fish Balls.					
30516277	5	90	theme	ZnO/TiO2	1085:1092	arg1	NPs					1094:1096	ZnO/TiO2 NPs	1085:1096	ZnO/TiO2 NPs	1085:1096	The preservation properties of the chitosan coatings for Silver Carp fish balls are improved by in situ SiOx, and further improved by co-modification of ZnO/TiO2 NPs.					
30516277	7	91	dep	APPLICATION	1225:1235	arg1	found					1278:1282	found	1278:1282	was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus	1274:1411	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	9	92	theme	carp	1701:1704	arg1	balls					1711:1715	silver carp fish balls	1694:1715	silver carp fish balls	1694:1715	In situ SiOx modified by ZnO/TiO2 NPs were synthesized, measured, and characterized in this study, and were applied for the preservation of silver carp fish balls.					
30516277	11	93	contain	have	1895:1898	arg2	potential					1900:1908	potential	1900:1908	potential as application in the food industry to guarantee food quality	1900:1970	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	11	93	contain	have	1895:1898	arg1	coatings					1886:1893	the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings	1849:1893	the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings	1849:1893	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	9	94	theme	balls	1711:1715	arg1	preservation					1678:1689	the preservation	1674:1689	the preservation of silver carp fish balls	1674:1715	In situ SiOx modified by ZnO/TiO2 NPs were synthesized, measured, and characterized in this study, and were applied for the preservation of silver carp fish balls.					
30516277	8	95	theme	nontoxic	1484:1491	arg1	materials					1505:1513	nontoxic edible nano materials	1484:1513	nontoxic edible nano materials	1484:1513	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	10	96	theme	preservation	1748:1759	arg1	material					1761:1768	a potential preservation material	1736:1768	a potential preservation material due to the increasing mechanical preservation properties	1736:1825	It could serve as a potential preservation material due to the increasing mechanical preservation properties.					
30516277	10	96	theme	preservation	1748:1759	arg1	It					1718:1719	It	1718:1719	It	1718:1719	It could serve as a potential preservation material due to the increasing mechanical preservation properties.					
30516277	9	97	theme	ZnO/TiO2	1579:1586	arg1	NPs					1588:1590	ZnO/TiO2 NPs	1579:1590	ZnO/TiO2 NPs	1579:1590	In situ SiOx modified by ZnO/TiO2 NPs were synthesized, measured, and characterized in this study, and were applied for the preservation of silver carp fish balls.					
30516277	0	98	dep	In	20:21	arg1	Situ					23:26	Situ	23:26	Situ	23:26	The Modification of In Situ SiOx Chitosan Coatings by ZnO/TiO2 NPs and Its Preservation Properties to Silver Carp Fish Balls.					
30516277	8	99	theme	nano	1500:1503	arg1	materials					1505:1513	nontoxic edible nano materials	1484:1513	nontoxic edible nano materials	1484:1513	In order to further improve properties of chitosan coatings, we added nontoxic edible nano materials to the in situ SiOx chitosan coatings.					
30516277	3	100	theme	chitosan	596:603	arg1	coatings					605:612	The chitosan coatings	592:612	The chitosan coatings modified by NPs	592:628	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	7	101	theme	ocellatus	1403:1411	arg1	time					1385:1388	shelf time	1379:1388	shelf time of Sciaenops ocellatus	1379:1411	PRACTICAL APPLICATION: In our previous study, in situ SiOx was found to improve antibacterial and preservation properties of chitosan coating, leading to extending shelf time of Sciaenops ocellatus.					
30516277	11	102	from	application	1913:1923	arg1	industry					1937:1944	the food industry	1928:1944	the food industry	1928:1944	Through the results, the ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coatings have potential as application in the food industry to guarantee food quality and extend shelf life of products.					
30516277	3	103	theme	few	637:639	arg1	cracks					641:646	few cracks	637:646	few cracks	637:646	The chitosan coatings modified by NPs showed few cracks, among which sodium hexametaphosphate (SHMP) modified ZnO/TiO2 /SiOx-chitosan (ZTS-CS) coating is proved to be the optimal one.					
30516277	1	104	theme	silver	236:241	arg1	carp					243:246	silver carp	236:246	silver carp fish balls	236:257	The composite chitosan coatings were prepared and characterized to evaluate their preservation properties for silver carp fish balls, and the microstructures and physicochemical properties of the coatings were improved by in situ nano silicon oxide (SiOx) and zinc oxide/titania (ZnO/TiO2 ) nano-particles (NPs).					
29961123	0	0	theme	mesenchymal	63:73	arg1	cells					80:84	mesenchymal stem cells	63:84	mesenchymal stem cells	63:84	Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces.					
29961123	3	1	theme	proteins	711:718	arg1	quantities					656:665	enhanced quantities	647:665	enhanced quantities of biologically available, surface-attached proteins	647:718	We showed that the increase of the biomaterials surface charge resulted in enhanced quantities of biologically available, surface-attached proteins.					
29961123	7	2	theme	artificial	1399:1408	arg1	organs					1410:1415	artificial organs	1399:1415	artificial organs	1399:1415	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	1	3	from	approaches	244:253	arg1	medicine					271:278	regenerative medicine	258:278	regenerative medicine	258:278	The surface charge of a biomaterial represents a promising tool to direct cellular behavior, which is crucial for therapeutic approaches in regenerative medicine.					
29961123	3	4	theme	available	683:691	arg1	proteins					711:718	biologically available, surface-attached proteins	670:718	biologically available, surface-attached proteins	670:718	We showed that the increase of the biomaterials surface charge resulted in enhanced quantities of biologically available, surface-attached proteins.					
29961123	4	5	theme	features	898:905	arg1	compositions					856:867	diverse protein compositions	840:867	diverse protein compositions instead of different surface features	840:905	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	6	6	theme	first	1136:1140	arg1	results					1142:1148	first results	1136:1148	first results	1136:1148	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	2	7	theme	surface	329:335	arg1	charge					337:342	the material surface charge	316:342	the material surface charge	316:342	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	0	8	theme	cells	80:84	arg1	behavior					51:58	the behavior	47:58	the behavior of mesenchymal stem cells with charged hydrogel surfaces	47:115	Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces.					
29961123	4	9	theme	stem	806:809	arg1	cells					811:815	mesenchymal stem cells	794:815	mesenchymal stem cells	794:815	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	7	10	theme	devices	1387:1393	arg1	design					1366:1371	the design	1362:1371	the design of biomedical devices and artificial organs	1362:1415	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	6	11	theme	cell	1220:1223	arg1	differentiation					1225:1239	mesenchymal stem cell differentiation	1203:1239	mesenchymal stem cell differentiation towards bone and fat cells	1203:1266	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	5	12	theme	adhesion-related	1035:1050	arg1	genes					1052:1056	adhesion-related genes integrin α5 and tensin 1	1035:1081	adhesion-related genes integrin α5 and tensin 1	1035:1081	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	1	13	theme	therapeutic	232:242	arg1	approaches					244:253	therapeutic approaches	232:253	therapeutic approaches in regenerative medicine	232:278	The surface charge of a biomaterial represents a promising tool to direct cellular behavior, which is crucial for therapeutic approaches in regenerative medicine.					
29961123	0	14	theme	stem	75:78	arg1	cells					80:84	mesenchymal stem cells	63:84	mesenchymal stem cells	63:84	Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces.					
29961123	6	15	theme	stem	1215:1218	arg1	differentiation					1225:1239	mesenchymal stem cell differentiation	1203:1239	mesenchymal stem cell differentiation towards bone and fat cells	1203:1266	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	0	16	with	behavior	51:58	arg1	surfaces					108:115	charged hydrogel surfaces	91:115	charged hydrogel surfaces	91:115	Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces.					
29961123	4	17	theme	protein	774:780	arg1	adsorption					782:791	protein adsorption	774:791	protein adsorption	774:791	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	7	18	theme	cell	1322:1325	arg1	behavior					1327:1334	surface charge-related cell behavior	1299:1334	surface charge-related cell behavior	1299:1334	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	0	19	theme	charged	91:97	arg1	surfaces					108:115	charged hydrogel surfaces	91:115	charged hydrogel surfaces	91:115	Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces.					
29961123	7	20	theme	behavior	1327:1334	arg1	understanding					1282:1294	The improved understanding	1269:1294	The improved understanding of surface charge-related cell behavior	1269:1334	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	4	21	theme	mesenchymal	794:804	arg1	cells					811:815	mesenchymal stem cells	794:815	mesenchymal stem cells	794:815	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	1	22	theme	biomaterial	142:152	arg1	charge					130:135	The surface charge	118:135	The surface charge of a biomaterial	118:152	The surface charge of a biomaterial represents a promising tool to direct cellular behavior, which is crucial for therapeutic approaches in regenerative medicine.					
29961123	7	23	contain	has	1336:1338	arg1	understanding					1282:1294	The improved understanding	1269:1294	The improved understanding of surface charge-related cell behavior	1269:1334	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	7	23	contain	has	1336:1338	arg2	impact					1352:1357	significant impact	1340:1357	significant impact	1340:1357	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	2	24	theme	mesenchymal	375:385	arg1	behavior					397:404	mesenchymal stem cell behavior	375:404	mesenchymal stem cell behavior	375:404	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	2	25	theme	zeta	441:444	arg1	potentials					446:455	zeta potentials	441:455	zeta potentials spanning from -25 mV to +15 mV	441:486	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	4	26	theme	surface	890:896	arg1	features					898:905	different surface features	880:905	different surface features	880:905	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	6	27	theme	charge	1193:1198	arg1	impact					1171:1176	a potential impact	1159:1176	a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells	1159:1266	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	3	28	dep	available	683:691	arg1	surface-attached					694:709	surface-attached	694:709	surface-attached	694:709	We showed that the increase of the biomaterials surface charge resulted in enhanced quantities of biologically available, surface-attached proteins.					
29961123	4	29	theme	different	880:888	arg1	features					898:905	different surface features	880:905	different surface features	880:905	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	6	30	theme	surface	1185:1191	arg1	charge					1193:1198	the surface charge	1181:1198	the surface charge	1181:1198	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	1	31	theme	promising	167:175	arg1	tool					177:180	a promising tool	165:180	a promising tool to direct cellular behavior, which is crucial for therapeutic approaches in regenerative medicine	165:278	The surface charge of a biomaterial represents a promising tool to direct cellular behavior, which is crucial for therapeutic approaches in regenerative medicine.					
29961123	1	32	theme	regenerative	258:269	arg1	medicine					271:278	regenerative medicine	258:278	regenerative medicine	258:278	The surface charge of a biomaterial represents a promising tool to direct cellular behavior, which is crucial for therapeutic approaches in regenerative medicine.					
29961123	6	33	theme	fat	1258:1260	arg1	cells					1262:1266	bone and fat cells	1249:1266	bone and fat cells	1249:1266	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	5	34	theme	cell	993:996	arg1	area					1008:1011	the cell spreading area	989:1011	the cell spreading area	989:1011	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	5	35	dep	genes	1052:1056	arg1	tensin					1074:1079	tensin 1	1074:1081	tensin 1	1074:1081	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	5	35	dep	genes	1052:1056	arg1	α5					1067:1068	integrin α5	1058:1068	integrin α5	1058:1068	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	2	36	theme	charged	419:425	arg1	surfaces					427:434	differently charged surfaces	407:434	differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV	407:486	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	0	37	theme	hydrogel	99:106	arg1	surfaces					108:115	charged hydrogel surfaces	91:115	charged hydrogel surfaces	91:115	Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces.					
29961123	5	38	theme	spreading	998:1006	arg1	area					1008:1011	the cell spreading area	989:1011	the cell spreading area	989:1011	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	1	39	theme	surface	122:128	arg1	charge					130:135	The surface charge	118:135	The surface charge of a biomaterial	118:152	The surface charge of a biomaterial represents a promising tool to direct cellular behavior, which is crucial for therapeutic approaches in regenerative medicine.					
29961123	4	40	theme	diverse	840:846	arg1	compositions					856:867	diverse protein compositions	840:867	diverse protein compositions instead of different surface features	840:905	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	7	41	theme	significant	1340:1350	arg1	impact					1352:1357	significant impact	1340:1357	significant impact	1340:1357	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	0	42	theme	-alginate	16:24	arg1	hydrogels					26:34	Poly(amidoamine)-alginate hydrogels	0:34	Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces.	0:116	Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces.					
29961123	2	43	theme	poly	526:529	arg1	conjugation					511:521	the conjugation	507:521	the conjugation of poly(amidoamine) to alginate-based hydrogels	507:569	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	3	44	theme	surface	620:626	arg1	charge					628:633	the biomaterials surface charge	603:633	the biomaterials surface charge	603:633	We showed that the increase of the biomaterials surface charge resulted in enhanced quantities of biologically available, surface-attached proteins.					
29961123	2	45	with	surfaces	427:434	arg1	potentials					446:455	zeta potentials	441:455	zeta potentials spanning from -25 mV to +15 mV	441:486	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	1	46	theme	cellular	192:199	arg1	behavior					201:208	cellular behavior	192:208	cellular behavior	192:208	The surface charge of a biomaterial represents a promising tool to direct cellular behavior, which is crucial for therapeutic approaches in regenerative medicine.					
29961123	5	47	theme	enhanced	919:926	arg1	attachment					933:942	an enhanced cell attachment	916:942	an enhanced cell attachment to increasingly positively charged surfaces	916:986	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	3	48	theme	charge	628:633	arg1	increase					591:598	the increase	587:598	the increase of the biomaterials surface charge	587:633	We showed that the increase of the biomaterials surface charge resulted in enhanced quantities of biologically available, surface-attached proteins.					
29961123	0	49	dep	hydrogels	26:34	arg1	directing					37:45	directing	37:45	directing the behavior of mesenchymal stem cells with charged hydrogel surfaces	37:115	Poly(amidoamine)-alginate hydrogels: directing the behavior of mesenchymal stem cells with charged hydrogel surfaces.					
29961123	2	50	theme	material	320:327	arg1	charge					337:342	the material surface charge	316:342	the material surface charge	316:342	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	2	51	theme	stem	387:390	arg1	behavior					397:404	mesenchymal stem cell behavior	375:404	mesenchymal stem cell behavior	375:404	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	4	52	theme	protein	848:854	arg1	compositions					856:867	diverse protein compositions	840:867	diverse protein compositions instead of different surface features	840:905	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	6	53	from	impact	1171:1176	arg1	differentiation					1225:1239	mesenchymal stem cell differentiation	1203:1239	mesenchymal stem cell differentiation towards bone and fat cells	1203:1266	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	6	54	theme	potential	1161:1169	arg1	impact					1171:1176	a potential impact	1159:1176	a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells	1159:1266	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	7	55	theme	surface	1299:1305	arg1	behavior					1327:1334	surface charge-related cell behavior	1299:1334	surface charge-related cell behavior	1299:1334	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	2	56	theme	alginate-based	546:559	arg1	hydrogels					561:569	alginate-based hydrogels	546:569	alginate-based hydrogels	546:569	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	7	57	theme	biomedical	1376:1385	arg1	devices					1387:1393	biomedical devices	1376:1393	biomedical devices	1376:1393	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	7	58	theme	organs	1410:1415	arg1	design					1366:1371	the design	1362:1371	the design of biomedical devices and artificial organs	1362:1415	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	4	59	theme	surface	737:743	arg1	charges					745:751	different surface charges	727:751	different surface charges	727:751	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	3	60	theme	enhanced	647:654	arg1	quantities					656:665	enhanced quantities	647:665	enhanced quantities of biologically available, surface-attached proteins	647:718	We showed that the increase of the biomaterials surface charge resulted in enhanced quantities of biologically available, surface-attached proteins.					
29961123	3	61	theme	biomaterials	607:618	arg1	charge					628:633	the biomaterials surface charge	603:633	the biomaterials surface charge	603:633	We showed that the increase of the biomaterials surface charge resulted in enhanced quantities of biologically available, surface-attached proteins.					
29961123	4	62	theme	different	727:735	arg1	charges					745:751	different surface charges	727:751	different surface charges	727:751	Since different surface charges were equalized after protein adsorption, mesenchymal stem cells interacted rather with diverse protein compositions instead of different surface features.					
29961123	6	63	theme	bone	1249:1252	arg1	cells					1262:1266	bone and fat cells	1249:1266	bone and fat cells	1249:1266	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	5	64	theme	charged	971:977	arg1	surfaces					979:986	increasingly positively charged surfaces	947:986	increasingly positively charged surfaces	947:986	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	2	65	theme	cell	392:395	arg1	behavior					397:404	mesenchymal stem cell behavior	375:404	mesenchymal stem cell behavior	375:404	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	5	66	theme	genes	1052:1056	arg1	area					1008:1011	the cell spreading area	989:1011	the cell spreading area	989:1011	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	5	66	theme	genes	1052:1056	arg1	expression					1021:1030	the expression	1017:1030	the expression of adhesion-related genes integrin α5 and tensin 1	1017:1081	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	2	67	theme	protein	352:358	arg1	adsorption					360:369	protein adsorption	352:369	protein adsorption	352:369	To expand the understanding of how the material surface charge affects protein adsorption and mesenchymal stem cell behavior, differently charged surfaces with zeta potentials spanning from -25 mV to +15 mV were fabricated by the conjugation of poly(amidoamine) to alginate-based hydrogels.					
29961123	6	68	theme	mesenchymal	1203:1213	arg1	differentiation					1225:1239	mesenchymal stem cell differentiation	1203:1239	mesenchymal stem cell differentiation towards bone and fat cells	1203:1266	Moreover, first results indicate a potential impact of the surface charge on mesenchymal stem cell differentiation towards bone and fat cells.					
29961123	5	69	theme	integrin	1058:1065	arg1	α5					1067:1068	integrin α5	1058:1068	integrin α5	1058:1068	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	7	70	theme	charge-related	1307:1320	arg1	behavior					1327:1334	surface charge-related cell behavior	1299:1334	surface charge-related cell behavior	1299:1334	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
29961123	5	71	theme	cell	928:931	arg1	attachment					933:942	an enhanced cell attachment	916:942	an enhanced cell attachment to increasingly positively charged surfaces	916:986	Besides an enhanced cell attachment to increasingly positively charged surfaces, the cell spreading area and the expression of adhesion-related genes integrin α5 and tensin 1 were found to be increased after adhesion.					
29961123	7	72	theme	improved	1273:1280	arg1	understanding					1282:1294	The improved understanding	1269:1294	The improved understanding of surface charge-related cell behavior	1269:1334	The improved understanding of surface charge-related cell behavior has significant impact on the design of biomedical devices and artificial organs.					
30863071	7	0	theme	hybrid	1136:1141	arg1	system					1143:1148	the irisin-loaded hybrid system	1118:1148	the irisin-loaded hybrid system (i/SCS/P system)	1118:1165	The release kinetics assay demonstrated that irisin was stably released from the irisin-loaded hybrid system (i/SCS/P system) to 50% within 7 days.					
30863071	7	0	theme	hybrid	1136:1141	arg1	system					1159:1164	i/SCS/P system	1151:1164	i/SCS/P system	1151:1164	The release kinetics assay demonstrated that irisin was stably released from the irisin-loaded hybrid system (i/SCS/P system) to 50% within 7 days.					
30863071	2	1	theme	molecular	339:347	arg1	entity					349:354	the molecular entity	335:354	the molecular entity responsible for muscle-bone connectivity	335:395	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	2	1	theme	molecular	339:347	arg1	irisin					319:324	irisin	319:324	irisin	319:324	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	4	2	from	irisin	699:704	arg1	SCS					713:715	the SCS	709:715	the SCS	709:715	Then we loaded irisin on the SCS before coating it with polyvinyl alcohol (PVA).					
30863071	1	3	theme	energy	264:269	arg1	expenditure					271:281	energy expenditure	264:281	energy expenditure	264:281	BACKGROUND Irisin is a cytokine produced by skeletal muscle and usually plays a pivotal role in inducing fat browning and regulating energy expenditure.					
30863071	8	4	theme	bone	1228:1231	arg1	BMSCs					1252:1256	BMSCs	1252:1256	BMSCs	1252:1256	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	4	theme	bone	1228:1231	arg1	cells					1245:1249	bone marrow stem cells	1228:1249	bone marrow stem cells (BMSCs)	1228:1257	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	5	theme	stem	1240:1243	arg1	BMSCs					1252:1256	BMSCs	1252:1256	BMSCs	1252:1256	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	5	theme	stem	1240:1243	arg1	cells					1245:1249	bone marrow stem cells	1228:1249	bone marrow stem cells (BMSCs)	1228:1257	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	10	6	theme	calvarial	1721:1729	arg1	defects					1731:1737	rat calvarial defects	1717:1737	rat calvarial defects	1717:1737	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	1	7	theme	pivotal	211:217	arg1	role					219:222	a pivotal role	209:222	a pivotal role	209:222	BACKGROUND Irisin is a cytokine produced by skeletal muscle and usually plays a pivotal role in inducing fat browning and regulating energy expenditure.					
30863071	8	8	theme	osteogenic	1399:1408	arg1	markers					1410:1416	osteogenic markers	1399:1416	osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN	1399:1468	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	7	9	theme	release	1045:1051	arg1	assay					1062:1066	The release kinetics assay	1041:1066	The release kinetics assay	1041:1066	The release kinetics assay demonstrated that irisin was stably released from the irisin-loaded hybrid system (i/SCS/P system) to 50% within 7 days.					
30863071	3	10	theme	interpenetrating	590:605	arg1	hydrogel					615:622	an interpenetrating network hydrogel	587:622	an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate	587:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	6	11	theme	electron	1011:1018	arg1	microscopy					1020:1029	scanning electron microscopy	1002:1029	scanning electron microscopy analysis	1002:1038	RESULTS The SCS/P scaffold was showed a porous sponge structure pursuant to scanning electron microscopy analysis.					
30863071	11	12	theme	bone	1887:1890	arg1	engineering					1899:1909	bone tissue engineering	1887:1909	bone tissue engineering	1887:1909	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	3	13	theme	silk	635:638	arg1	fibroin					640:646	silk fibroin	635:646	silk fibroin	635:646	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	13	theme	silk	635:638	arg1	silicate					657:664	calcium silicate	649:664	calcium silicate	649:664	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	13	theme	silk	635:638	arg1	alginate					674:681	sodium alginate	667:681	sodium alginate	667:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	0	14	theme	composite	89:97	arg1	scaffold					99:106	silk/calcium silicate/sodium alginate composite scaffold	51:106	silk/calcium silicate/sodium alginate composite scaffold for bone regeneration	51:128	Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration.					
30863071	5	15	theme	scaffold	888:895	arg1	effect					897:902	the scaffold effect	884:902	the scaffold effect on bone regeneration	884:923	The SCS/P scaffold was physically characterized and some in vitro and in vivo experiments were carried out to evaluate the scaffold effect on bone regeneration.					
30863071	9	16	theme	red	1480:1482	arg1	staining					1484:1491	Alizarin red staining	1471:1491	Alizarin red staining	1471:1491	Alizarin red staining also demonstrated the promotion of osteogenesis induced by i/SCS/P scaffolds.					
30863071	6	17	theme	porous	966:971	arg1	structure					980:988	a porous sponge structure	964:988	a porous sponge structure pursuant to scanning electron microscopy analysis	964:1038	RESULTS The SCS/P scaffold was showed a porous sponge structure pursuant to scanning electron microscopy analysis.					
30863071	2	18	theme	osteogenesis	414:425	arg1	induction					427:435	osteogenesis induction	414:435	osteogenesis induction	414:435	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	8	19	theme	phosphatase	1331:1341	arg1	activity					1310:1317	the activity	1306:1317	the activity of alkaline phosphatase (ALP)	1306:1347	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	11	20	dep	CONCLUSION	1777:1786	arg1	provided					1799:1806	provided	1799:1806	provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering	1799:1909	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	4	21	theme	polyvinyl	740:748	arg1	alcohol					750:756	polyvinyl alcohol	740:756	polyvinyl alcohol (PVA)	740:762	Then we loaded irisin on the SCS before coating it with polyvinyl alcohol (PVA).					
30863071	4	21	theme	polyvinyl	740:748	arg1	PVA					759:761	PVA	759:761	PVA	759:761	Then we loaded irisin on the SCS before coating it with polyvinyl alcohol (PVA).					
30863071	10	22	theme	in	1584:1585	arg1	studies					1592:1598	in vivo studies	1584:1598	in vivo studies	1584:1598	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	0	23	theme	Delivery	0:7	arg1	vehicle					9:15	Delivery vehicle	0:15	Delivery vehicle of muscle-derived irisin	0:40	Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration.					
30863071	5	24	from	effect	897:902	arg1	regeneration					912:923	bone regeneration	907:923	bone regeneration	907:923	The SCS/P scaffold was physically characterized and some in vitro and in vivo experiments were carried out to evaluate the scaffold effect on bone regeneration.					
30863071	0	25	dep	silk/calcium	51:62	arg1	silicate/sodium					64:78	silicate/sodium	64:78	silicate/sodium	64:78	Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration.					
30863071	10	26	theme	bone	1622:1625	arg1	regeneration					1627:1638	increased bone regeneration	1612:1638	increased bone regeneration with better mineralization and higher quality	1612:1684	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	5	27	theme	in	822:823	arg1	experiments					843:853	some in vitro and in vivo experiments	817:853	some in vitro and in vivo experiments	817:853	The SCS/P scaffold was physically characterized and some in vitro and in vivo experiments were carried out to evaluate the scaffold effect on bone regeneration.					
30863071	3	28	theme	alginate	544:551	arg1	scaffold					569:576	silk/calcium silicate/sodium alginate (SCS) composite scaffold	515:576	silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate	515:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	29	theme	sodium	667:672	arg1	fibroin					640:646	silk fibroin	635:646	silk fibroin	635:646	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	29	theme	sodium	667:672	arg1	alginate					674:681	sodium alginate	667:681	sodium alginate	667:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	0	30	theme	irisin	35:40	arg1	vehicle					9:15	Delivery vehicle	0:15	Delivery vehicle of muscle-derived irisin	0:40	Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration.					
30863071	11	31	theme	strong	1808:1813	arg1	evidence					1815:1822	strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering	1808:1909	strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering	1808:1909	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	9	32	theme	i/SCS/P	1552:1558	arg1	scaffolds					1560:1568	i/SCS/P scaffolds	1552:1568	i/SCS/P scaffolds	1552:1568	Alizarin red staining also demonstrated the promotion of osteogenesis induced by i/SCS/P scaffolds.					
30863071	7	33	attach	released	1104:1111	arg2	irisin					1086:1091	irisin	1086:1091	irisin	1086:1091	The release kinetics assay demonstrated that irisin was stably released from the irisin-loaded hybrid system (i/SCS/P system) to 50% within 7 days.					
30863071	7	33	attach	released	1104:1111	arg1	system					1143:1148	the irisin-loaded hybrid system	1118:1148	the irisin-loaded hybrid system (i/SCS/P system)	1118:1165	The release kinetics assay demonstrated that irisin was stably released from the irisin-loaded hybrid system (i/SCS/P system) to 50% within 7 days.					
30863071	7	33	attach	released	1104:1111	arg1	system					1159:1164	i/SCS/P system	1151:1164	i/SCS/P system	1151:1164	The release kinetics assay demonstrated that irisin was stably released from the irisin-loaded hybrid system (i/SCS/P system) to 50% within 7 days.					
30863071	10	34	dep	in	1584:1585	arg1	vivo					1587:1590	vivo	1587:1590	vivo	1587:1590	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	3	35	from	effect	473:478	arg1	regeneration					488:499	bone regeneration	483:499	bone regeneration	483:499	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	8	36	dep	exhibited	1268:1276	arg1	enhanced					1353:1360	enhanced	1353:1360	enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN	1353:1468	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	36	dep	exhibited	1268:1276	arg1	improved					1297:1304	improved	1297:1304	improved the activity of alkaline phosphatase (ALP)	1297:1347	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	3	37	contain	containing	624:633	arg1	hydrogel					615:622	an interpenetrating network hydrogel	587:622	an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate	587:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	37	contain	containing	624:633	arg2	alginate					674:681	sodium alginate	667:681	sodium alginate	667:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	37	contain	containing	624:633	arg2	silicate					657:664	calcium silicate	649:664	calcium silicate	649:664	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	37	contain	containing	624:633	arg2	fibroin					640:646	silk fibroin	635:646	silk fibroin	635:646	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	38	theme	silk/calcium	515:526	arg1	scaffold					569:576	silk/calcium silicate/sodium alginate (SCS) composite scaffold	515:576	silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate	515:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	2	39	from	induction	427:435	arg1	useful					404:409	useful	404:409	useful	404:409	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	8	40	contain	containing	1418:1427	arg2	ALP					1437:1439	ALP	1437:1439	ALP	1437:1439	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	40	contain	containing	1418:1427	arg2	OCN					1457:1459	OCN	1457:1459	OCN	1457:1459	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	40	contain	containing	1418:1427	arg1	markers					1410:1416	osteogenic markers	1399:1416	osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN	1399:1468	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	40	contain	containing	1418:1427	arg2	BMP2					1442:1445	BMP2	1442:1445	BMP2	1442:1445	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	40	contain	containing	1418:1427	arg2	OPN					1466:1468	OPN	1466:1468	OPN	1466:1468	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	40	contain	containing	1418:1427	arg2	Runx-2					1429:1434	Runx-2	1429:1434	Runx-2	1429:1434	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	40	contain	containing	1418:1427	arg2	Osterix					1448:1454	Osterix	1448:1454	Osterix	1448:1454	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	7	41	theme	irisin-loaded	1122:1134	arg1	system					1143:1148	the irisin-loaded hybrid system	1118:1148	the irisin-loaded hybrid system (i/SCS/P system)	1118:1165	The release kinetics assay demonstrated that irisin was stably released from the irisin-loaded hybrid system (i/SCS/P system) to 50% within 7 days.					
30863071	7	41	theme	irisin-loaded	1122:1134	arg1	system					1159:1164	i/SCS/P system	1151:1164	i/SCS/P system	1151:1164	The release kinetics assay demonstrated that irisin was stably released from the irisin-loaded hybrid system (i/SCS/P system) to 50% within 7 days.					
30863071	10	42	theme	higher	1671:1676	arg1	quality					1678:1684	higher quality	1671:1684	higher quality	1671:1684	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	5	43	dep	in	822:823	arg1	vitro					825:829	vitro	825:829	vitro	825:829	The SCS/P scaffold was physically characterized and some in vitro and in vivo experiments were carried out to evaluate the scaffold effect on bone regeneration.					
30863071	0	44	link	muscle-derived	20:33	arg1	irisin					35:40	muscle-derived irisin	20:40	muscle-derived irisin	20:40	Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration.					
30863071	3	45	theme	SCS	554:556	arg1	scaffold					569:576	silk/calcium silicate/sodium alginate (SCS) composite scaffold	515:576	silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate	515:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	2	46	theme	recent	287:292	arg1	years					294:298	recent years	287:298	recent years	287:298	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	10	47	theme	defects	1731:1737	arg1	repair					1707:1712	the repair	1703:1712	the repair of rat calvarial defects	1703:1737	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	8	48	theme	marrow	1233:1238	arg1	BMSCs					1252:1256	BMSCs	1252:1256	BMSCs	1252:1256	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	48	theme	marrow	1233:1238	arg1	cells					1245:1249	bone marrow stem cells	1228:1249	bone marrow stem cells (BMSCs)	1228:1257	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	11	49	dep	promising	1862:1870	arg1	promising					1862:1870	promising	1862:1870	promising	1862:1870	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	11	49	dep	promising	1862:1870	arg1	substitute					1872:1881	substitute	1872:1881	substitute for bone tissue engineering	1872:1909	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	11	49	dep	promising	1862:1870	arg1	i/SCS/P					1843:1849	the composite i/SCS/P	1829:1849	the composite i/SCS/P	1829:1849	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	3	50	theme	composite	559:567	arg1	scaffold					569:576	silk/calcium silicate/sodium alginate (SCS) composite scaffold	515:576	silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate	515:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	6	51	dep	RESULTS	926:932	arg1	showed					957:962	showed	957:962	was showed a porous sponge structure pursuant to scanning electron microscopy analysis	953:1038	RESULTS The SCS/P scaffold was showed a porous sponge structure pursuant to scanning electron microscopy analysis.					
30863071	1	52	theme	skeletal	175:182	arg1	muscle					184:189	skeletal muscle	175:189	skeletal muscle	175:189	BACKGROUND Irisin is a cytokine produced by skeletal muscle and usually plays a pivotal role in inducing fat browning and regulating energy expenditure.					
30863071	7	53	theme	kinetics	1053:1060	arg1	assay					1062:1066	The release kinetics assay	1041:1066	The release kinetics assay	1041:1066	The release kinetics assay demonstrated that irisin was stably released from the irisin-loaded hybrid system (i/SCS/P system) to 50% within 7 days.					
30863071	10	54	theme	i/SCS/P	1761:1767	arg1	system					1769:1774	the i/SCS/P system	1757:1774	the i/SCS/P system	1757:1774	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	5	55	theme	in	835:836	arg1	experiments					843:853	some in vitro and in vivo experiments	817:853	some in vitro and in vivo experiments	817:853	The SCS/P scaffold was physically characterized and some in vitro and in vivo experiments were carried out to evaluate the scaffold effect on bone regeneration.					
30863071	6	56	theme	SCS/P	938:942	arg1	scaffold					944:951	The SCS/P scaffold	934:951	The SCS/P scaffold	934:951	RESULTS The SCS/P scaffold was showed a porous sponge structure pursuant to scanning electron microscopy analysis.					
30863071	1	57	theme	BACKGROUND	131:140	arg1	Irisin					142:147	BACKGROUND Irisin	131:147	BACKGROUND Irisin	131:147	BACKGROUND Irisin is a cytokine produced by skeletal muscle and usually plays a pivotal role in inducing fat browning and regulating energy expenditure.					
30863071	1	57	theme	BACKGROUND	131:140	arg1	cytokine					154:161	a cytokine	152:161	a cytokine produced by skeletal muscle	152:189	BACKGROUND Irisin is a cytokine produced by skeletal muscle and usually plays a pivotal role in inducing fat browning and regulating energy expenditure.					
30863071	8	58	theme	osteoinductive	1199:1212	arg1	studies					1214:1220	osteoinductive studies	1199:1220	osteoinductive studies using bone marrow stem cells (BMSCs) in vitro	1199:1266	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	5	59	dep	in	835:836	arg1	vivo					838:841	vivo	838:841	vivo	838:841	The SCS/P scaffold was physically characterized and some in vitro and in vivo experiments were carried out to evaluate the scaffold effect on bone regeneration.					
30863071	5	60	theme	bone	907:910	arg1	regeneration					912:923	bone regeneration	907:923	bone regeneration	907:923	The SCS/P scaffold was physically characterized and some in vitro and in vivo experiments were carried out to evaluate the scaffold effect on bone regeneration.					
30863071	10	61	theme	rat	1717:1719	arg1	defects					1731:1737	rat calvarial defects	1717:1737	rat calvarial defects	1717:1737	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	6	62	theme	microscopy	1020:1029	arg1	analysis					1031:1038	scanning electron microscopy analysis	1002:1038	scanning electron microscopy analysis	1002:1038	RESULTS The SCS/P scaffold was showed a porous sponge structure pursuant to scanning electron microscopy analysis.					
30863071	0	63	theme	alginate	80:87	arg1	scaffold					99:106	silk/calcium silicate/sodium alginate composite scaffold	51:106	silk/calcium silicate/sodium alginate composite scaffold for bone regeneration	51:128	Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration.					
30863071	6	64	theme	scanning	1002:1009	arg1	microscopy					1020:1029	scanning electron microscopy	1002:1029	scanning electron microscopy analysis	1002:1038	RESULTS The SCS/P scaffold was showed a porous sponge structure pursuant to scanning electron microscopy analysis.					
30863071	11	65	theme	tissue	1892:1897	arg1	engineering					1899:1909	bone tissue engineering	1887:1909	bone tissue engineering	1887:1909	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	8	66	theme	i/SCS/P	1282:1288	arg1	system					1290:1295	the i/SCS/P system	1278:1295	the i/SCS/P system	1278:1295	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	67	theme	markers	1410:1416	arg1	series					1389:1394	a series	1387:1394	a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN	1387:1468	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	0	68	theme	bone	112:115	arg1	regeneration					117:128	bone regeneration	112:128	bone regeneration	112:128	Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration.					
30863071	9	69	theme	Alizarin	1471:1478	arg1	staining					1484:1491	Alizarin red staining	1471:1491	Alizarin red staining	1471:1491	Alizarin red staining also demonstrated the promotion of osteogenesis induced by i/SCS/P scaffolds.					
30863071	6	70	theme	sponge	973:978	arg1	structure					980:988	a porous sponge structure	964:988	a porous sponge structure pursuant to scanning electron microscopy analysis	964:1038	RESULTS The SCS/P scaffold was showed a porous sponge structure pursuant to scanning electron microscopy analysis.					
30863071	8	71	theme	alkaline	1322:1329	arg1	ALP					1344:1346	ALP	1344:1346	ALP	1344:1346	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	8	71	theme	alkaline	1322:1329	arg1	phosphatase					1331:1341	alkaline phosphatase	1322:1341	alkaline phosphatase (ALP)	1322:1347	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	11	72	theme	composite	1833:1841	arg1	promising					1862:1870	promising	1862:1870	promising	1862:1870	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	11	72	theme	composite	1833:1841	arg1	substitute					1872:1881	substitute	1872:1881	substitute for bone tissue engineering	1872:1909	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	11	72	theme	composite	1833:1841	arg1	i/SCS/P					1843:1849	the composite i/SCS/P	1829:1849	the composite i/SCS/P	1829:1849	CONCLUSION These data provided strong evidence that the composite i/SCS/P would be a promising substitute for bone tissue engineering.					
30863071	2	73	located	found	308:312	arg1	years					294:298	recent years	287:298	recent years	287:298	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	2	73	located	found	308:312	arg2	it					301:302	it	301:302	it	301:302	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	2	74	theme	muscle-bone	372:382	arg1	connectivity					384:395	muscle-bone connectivity	372:395	muscle-bone connectivity	372:395	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	0	75	theme	muscle-derived	20:33	arg1	irisin					35:40	muscle-derived irisin	20:40	muscle-derived irisin	20:40	Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration.					
30863071	3	76	dep	silk/calcium	515:526	arg1	silicate/sodium					528:542	silicate/sodium	528:542	silicate/sodium	528:542	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	77	theme	network	607:613	arg1	hydrogel					615:622	an interpenetrating network hydrogel	587:622	an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate	587:681	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	10	78	theme	increased	1612:1620	arg1	regeneration					1627:1638	increased bone regeneration	1612:1638	increased bone regeneration with better mineralization and higher quality	1612:1684	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	5	79	theme	SCS/P	769:773	arg1	scaffold					775:782	The SCS/P scaffold	765:782	The SCS/P scaffold	765:782	The SCS/P scaffold was physically characterized and some in vitro and in vivo experiments were carried out to evaluate the scaffold effect on bone regeneration.					
30863071	10	80	with	regeneration	1627:1638	arg1	mineralization					1652:1665	better mineralization	1645:1665	better mineralization	1645:1665	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	10	80	with	regeneration	1627:1638	arg1	quality					1678:1684	higher quality	1671:1684	higher quality	1671:1684	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	9	81	theme	osteogenesis	1528:1539	arg1	promotion					1515:1523	the promotion	1511:1523	the promotion of osteogenesis induced by i/SCS/P scaffolds	1511:1568	Alizarin red staining also demonstrated the promotion of osteogenesis induced by i/SCS/P scaffolds.					
30863071	3	82	theme	calcium	649:655	arg1	fibroin					640:646	silk fibroin	635:646	silk fibroin	635:646	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	3	82	theme	calcium	649:655	arg1	silicate					657:664	calcium silicate	649:664	calcium silicate	649:664	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	0	83	theme	silk/calcium	51:62	arg1	scaffold					99:106	silk/calcium silicate/sodium alginate composite scaffold	51:106	silk/calcium silicate/sodium alginate composite scaffold for bone regeneration	51:128	Delivery vehicle of muscle-derived irisin based on silk/calcium silicate/sodium alginate composite scaffold for bone regeneration.					
30863071	8	84	theme	expression	1366:1375	arg1	levels					1377:1382	the expression levels	1362:1382	the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN	1362:1468	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	10	85	theme	better	1645:1650	arg1	mineralization					1652:1665	better mineralization	1645:1665	better mineralization	1645:1665	In addition, in vivo studies showed that increased bone regeneration with better mineralization and higher quality was found during the repair of rat calvarial defects through utilizing the i/SCS/P system.					
30863071	3	86	theme	bone	483:486	arg1	regeneration					488:499	bone regeneration	483:499	bone regeneration	483:499	MATERIALS AND METHODS To study its effect on bone regeneration, we developed silk/calcium silicate/sodium alginate (SCS) composite scaffold based on an interpenetrating network hydrogel containing silk fibroin, calcium silicate, sodium alginate.					
30863071	1	87	theme	fat	236:238	arg1	browning					240:247	fat browning	236:247	fat browning	236:247	BACKGROUND Irisin is a cytokine produced by skeletal muscle and usually plays a pivotal role in inducing fat browning and regulating energy expenditure.					
30863071	2	88	from	useful	404:409	arg1	induction					427:435	osteogenesis induction	414:435	osteogenesis induction	414:435	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	8	89	theme	series	1389:1394	arg1	levels					1377:1382	the expression levels	1362:1382	the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN	1362:1468	Moreover, osteoinductive studies using bone marrow stem cells (BMSCs) in vitro exhibited the i/SCS/P system improved the activity of alkaline phosphatase (ALP) and enhanced the expression levels of a series of osteogenic markers containing Runx-2, ALP, BMP2, Osterix, OCN, and OPN.					
30863071	2	90	theme	responsible	356:366	arg1	entity					349:354	the molecular entity	335:354	the molecular entity responsible for muscle-bone connectivity	335:395	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30863071	2	90	theme	responsible	356:366	arg1	irisin					319:324	irisin	319:324	irisin	319:324	In recent years, it was found that irisin might be the molecular entity responsible for muscle-bone connectivity and is useful in osteogenesis induction.					
30287377	1	0	theme	polyurethane/chitosan	141:161	arg1	PU/chitosan					169:179	PU/chitosan	169:179	PU/chitosan	169:179	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	1	0	theme	polyurethane/chitosan	141:161	arg1	foam					163:166	A novel bio-based polyurethane/chitosan foam	123:166	A novel bio-based polyurethane/chitosan foam (PU/chitosan)	123:180	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	8	1	theme	267.24 mg g-1	976:988	arg1	capacity					964:971	a maximum adsorption capacity	943:971	a maximum adsorption capacity of 267.24 mg g-1	943:988	The equilibrium data followed the Sips model, with a maximum adsorption capacity of 267.24 mg g-1.					
30287377	6	2	theme	PU/chitosan	765:775	arg1	foam					787:790	PU/chitosan composite foam	765:790	PU/chitosan composite foam	765:790	The adsorption of FR17 on PU/chitosan composite foam was favored at pH 2.					
30287377	3	3	theme	mechanical	519:528	arg1	properties					530:539	good mechanical properties	514:539	good mechanical properties	514:539	PU/chitosan foam showed a semi-crystalline structure, with several functional groups, high porosity and good mechanical properties.					
30287377	10	4	theme	anionic	1226:1232	arg1	dyes					1234:1237	anionic dyes	1226:1237	anionic dyes	1226:1237	The results showed that polyurethane foams are capable to support chitosan, generating an adsorbent with better mechanical characteristics and high potential to remove anionic dyes from aqueous media.					
30287377	10	5	theme	mechanical	1170:1179	arg1	characteristics					1181:1195	better mechanical characteristics	1163:1195	better mechanical characteristics	1163:1195	The results showed that polyurethane foams are capable to support chitosan, generating an adsorbent with better mechanical characteristics and high potential to remove anionic dyes from aqueous media.					
30287377	5	6	theme	identical	611:619	arg1	conditions					632:641	identical adsorption conditions	611:641	identical adsorption conditions	611:641	Using identical adsorption conditions, PU/chitosan was able to remove >98% of FR17 dye from the solution, while, PU removed only 40%.					
30287377	7	7	theme	order	827:831	arg1	adequate					852:859	adequate	852:859	adequate	852:859	Pseudo-second order model was the most adequate to represent the kinetic data.					
30287377	7	7	theme	order	827:831	arg1	model					833:837	Pseudo-second order model	813:837	Pseudo-second order model	813:837	Pseudo-second order model was the most adequate to represent the kinetic data.					
30287377	4	8	theme	adsorptive	581:590	arg1	separations					592:602	adsorptive separations	581:602	adsorptive separations	581:602	These characteristics are adequate for adsorptive separations.					
30287377	0	9	theme	Red	111:113	arg1	dye					118:120	Food Red 17 dye	106:120	Food Red 17 dye	106:120	Synthesis of a bio-based polyurethane/chitosan composite foam using ricinoleic acid for the adsorption of Food Red 17 dye.					
30287377	3	10	theme	several	469:475	arg1	groups					488:493	several functional groups	469:493	several functional groups	469:493	PU/chitosan foam showed a semi-crystalline structure, with several functional groups, high porosity and good mechanical properties.					
30287377	5	11	theme	adsorption	621:630	arg1	conditions					632:641	identical adsorption conditions	611:641	identical adsorption conditions	611:641	Using identical adsorption conditions, PU/chitosan was able to remove >98% of FR17 dye from the solution, while, PU removed only 40%.					
30287377	3	12	theme	PU/chitosan	410:420	arg1	foam					422:425	PU/chitosan foam	410:425	PU/chitosan foam	410:425	PU/chitosan foam showed a semi-crystalline structure, with several functional groups, high porosity and good mechanical properties.					
30287377	0	13	theme	Food	106:109	arg1	dye					118:120	Food Red 17 dye	106:120	Food Red 17 dye	106:120	Synthesis of a bio-based polyurethane/chitosan composite foam using ricinoleic acid for the adsorption of Food Red 17 dye.					
30287377	6	14	theme	FR17	757:760	arg1	adsorption					743:752	The adsorption	739:752	The adsorption of FR17 on PU/chitosan composite foam	739:790	The adsorption of FR17 on PU/chitosan composite foam was favored at pH 2.					
30287377	3	15	theme	good	514:517	arg1	properties					530:539	good mechanical properties	514:539	good mechanical properties	514:539	PU/chitosan foam showed a semi-crystalline structure, with several functional groups, high porosity and good mechanical properties.					
30287377	8	16	theme	maximum	945:951	arg1	capacity					964:971	a maximum adsorption capacity	943:971	a maximum adsorption capacity of 267.24 mg g-1	943:988	The equilibrium data followed the Sips model, with a maximum adsorption capacity of 267.24 mg g-1.					
30287377	7	17	theme	Pseudo-second	813:825	arg1	adequate					852:859	adequate	852:859	adequate	852:859	Pseudo-second order model was the most adequate to represent the kinetic data.					
30287377	7	17	theme	Pseudo-second	813:825	arg1	model					833:837	Pseudo-second order model	813:837	Pseudo-second order model	813:837	Pseudo-second order model was the most adequate to represent the kinetic data.					
30287377	2	18	theme	adsorption	360:369	arg1	potential					371:379	adsorption potential	360:379	adsorption potential	360:379	PU/chitosan foam presented better characteristics and adsorption potential than polyurethane foam (PU).					
30287377	1	19	theme	Food	259:262	arg1	FR17					276:279	FR17	276:279	FR17	276:279	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	1	19	theme	Food	259:262	arg1	dye					271:273	Food Red 17 dye	259:273	Food Red 17 dye (FR17)	259:280	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	0	20	theme	bio-based	15:23	arg1	foam					57:60	a bio-based polyurethane/chitosan composite foam	13:60	a bio-based polyurethane/chitosan composite foam	13:60	Synthesis of a bio-based polyurethane/chitosan composite foam using ricinoleic acid for the adsorption of Food Red 17 dye.					
30287377	1	21	theme	Red	264:266	arg1	FR17					276:279	FR17	276:279	FR17	276:279	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	1	21	theme	Red	264:266	arg1	dye					271:273	Food Red 17 dye	259:273	Food Red 17 dye (FR17)	259:280	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	0	22	theme	dye	118:120	arg1	adsorption					92:101	the adsorption	88:101	the adsorption of Food Red 17 dye	88:120	Synthesis of a bio-based polyurethane/chitosan composite foam using ricinoleic acid for the adsorption of Food Red 17 dye.					
30287377	3	23	theme	semi-crystalline	436:451	arg1	structure					453:461	a semi-crystalline structure	434:461	a semi-crystalline structure	434:461	PU/chitosan foam showed a semi-crystalline structure, with several functional groups, high porosity and good mechanical properties.					
30287377	10	24	theme	better	1163:1168	arg1	characteristics					1181:1195	better mechanical characteristics	1163:1195	better mechanical characteristics	1163:1195	The results showed that polyurethane foams are capable to support chitosan, generating an adsorbent with better mechanical characteristics and high potential to remove anionic dyes from aqueous media.					
30287377	3	25	theme	high	496:499	arg1	porosity					501:508	high porosity	496:508	high porosity	496:508	PU/chitosan foam showed a semi-crystalline structure, with several functional groups, high porosity and good mechanical properties.					
30287377	0	26	theme	composite	47:55	arg1	foam					57:60	a bio-based polyurethane/chitosan composite foam	13:60	a bio-based polyurethane/chitosan composite foam	13:60	Synthesis of a bio-based polyurethane/chitosan composite foam using ricinoleic acid for the adsorption of Food Red 17 dye.					
30287377	10	27	theme	polyurethane	1082:1093	arg1	foams					1095:1099	polyurethane foams	1082:1099	polyurethane foams	1082:1099	The results showed that polyurethane foams are capable to support chitosan, generating an adsorbent with better mechanical characteristics and high potential to remove anionic dyes from aqueous media.					
30287377	10	28	with	adsorbent	1148:1156	arg1	characteristics					1181:1195	better mechanical characteristics	1163:1195	better mechanical characteristics	1163:1195	The results showed that polyurethane foams are capable to support chitosan, generating an adsorbent with better mechanical characteristics and high potential to remove anionic dyes from aqueous media.					
30287377	10	28	with	adsorbent	1148:1156	arg1	potential					1206:1214	high potential	1201:1214	high potential	1201:1214	The results showed that polyurethane foams are capable to support chitosan, generating an adsorbent with better mechanical characteristics and high potential to remove anionic dyes from aqueous media.					
30287377	10	29	theme	high	1201:1204	arg1	potential					1206:1214	high potential	1201:1214	high potential	1201:1214	The results showed that polyurethane foams are capable to support chitosan, generating an adsorbent with better mechanical characteristics and high potential to remove anionic dyes from aqueous media.					
30287377	0	30	theme	polyurethane/chitosan	25:45	arg1	foam					57:60	a bio-based polyurethane/chitosan composite foam	13:60	a bio-based polyurethane/chitosan composite foam	13:60	Synthesis of a bio-based polyurethane/chitosan composite foam using ricinoleic acid for the adsorption of Food Red 17 dye.					
30287377	1	31	attach	derived	213:219	arg2	polyol					206:211	a polyol	204:211	a polyol derived from castor oil	204:235	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	1	31	attach	derived	213:219	arg1	oil					233:235	castor oil	226:235	castor oil	226:235	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	2	32	theme	polyurethane	386:397	arg1	foam					399:402	polyurethane foam	386:402	polyurethane foam (PU)	386:407	PU/chitosan foam presented better characteristics and adsorption potential than polyurethane foam (PU).					
30287377	2	32	theme	polyurethane	386:397	arg1	PU					405:406	PU	405:406	PU	405:406	PU/chitosan foam presented better characteristics and adsorption potential than polyurethane foam (PU).					
30287377	2	33	theme	PU/chitosan	306:316	arg1	foam					318:321	PU/chitosan foam	306:321	PU/chitosan foam	306:321	PU/chitosan foam presented better characteristics and adsorption potential than polyurethane foam (PU).					
30287377	0	34	theme	foam	57:60	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a bio-based polyurethane/chitosan composite foam using ricinoleic acid for the adsorption of Food Red 17 dye.	0:121	Synthesis of a bio-based polyurethane/chitosan composite foam using ricinoleic acid for the adsorption of Food Red 17 dye.					
30287377	8	35	theme	equilibrium	896:906	arg1	data					908:911	The equilibrium data	892:911	The equilibrium data	892:911	The equilibrium data followed the Sips model, with a maximum adsorption capacity of 267.24 mg g-1.					
30287377	3	36	theme	functional	477:486	arg1	groups					488:493	several functional groups	469:493	several functional groups	469:493	PU/chitosan foam showed a semi-crystalline structure, with several functional groups, high porosity and good mechanical properties.					
30287377	8	37	theme	adsorption	953:962	arg1	capacity					964:971	a maximum adsorption capacity	943:971	a maximum adsorption capacity of 267.24 mg g-1	943:988	The equilibrium data followed the Sips model, with a maximum adsorption capacity of 267.24 mg g-1.					
30287377	6	38	theme	composite	777:785	arg1	foam					787:790	PU/chitosan composite foam	765:790	PU/chitosan composite foam	765:790	The adsorption of FR17 on PU/chitosan composite foam was favored at pH 2.					
30287377	7	39	theme	kinetic	878:884	arg1	data					886:889	the kinetic data	874:889	the kinetic data	874:889	Pseudo-second order model was the most adequate to represent the kinetic data.					
30287377	5	40	theme	dye	688:690	arg1	%					678:678	>98%	675:678	>98% of FR17 dye	675:690	Using identical adsorption conditions, PU/chitosan was able to remove >98% of FR17 dye from the solution, while, PU removed only 40%.					
30287377	5	40	theme	dye	688:690	arg1	dye					688:690	FR17 dye	683:690	FR17 dye	683:690	Using identical adsorption conditions, PU/chitosan was able to remove >98% of FR17 dye from the solution, while, PU removed only 40%.					
30287377	1	41	theme	bio-based	131:139	arg1	PU/chitosan					169:179	PU/chitosan	169:179	PU/chitosan	169:179	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	1	41	theme	bio-based	131:139	arg1	foam					163:166	A novel bio-based polyurethane/chitosan foam	123:166	A novel bio-based polyurethane/chitosan foam (PU/chitosan)	123:180	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	6	42	from	adsorption	743:752	arg1	foam					787:790	PU/chitosan composite foam	765:790	PU/chitosan composite foam	765:790	The adsorption of FR17 on PU/chitosan composite foam was favored at pH 2.					
30287377	1	43	theme	novel	125:129	arg1	PU/chitosan					169:179	PU/chitosan	169:179	PU/chitosan	169:179	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	1	43	theme	novel	125:129	arg1	foam					163:166	A novel bio-based polyurethane/chitosan foam	123:166	A novel bio-based polyurethane/chitosan foam (PU/chitosan)	123:180	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	9	44	theme	adsorption	995:1004	arg1	spontaneous					1018:1028	spontaneous	1018:1028	spontaneous	1018:1028	The adsorption process was spontaneous, favorable and endothermic.					
30287377	9	44	theme	adsorption	995:1004	arg1	process					1006:1012	The adsorption process	991:1012	The adsorption process	991:1012	The adsorption process was spontaneous, favorable and endothermic.					
30287377	1	45	theme	castor	226:231	arg1	oil					233:235	castor oil	226:235	castor oil	226:235	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	0	46	theme	ricinoleic	68:77	arg1	acid					79:82	ricinoleic acid	68:82	ricinoleic acid for the adsorption of Food Red 17 dye	68:120	Synthesis of a bio-based polyurethane/chitosan composite foam using ricinoleic acid for the adsorption of Food Red 17 dye.					
30287377	1	47	theme	aqueous	287:293	arg1	solutions					295:303	aqueous solutions	287:303	aqueous solutions	287:303	A novel bio-based polyurethane/chitosan foam (PU/chitosan) was synthesized using a polyol derived from castor oil and applied to remove Food Red 17 dye (FR17) from aqueous solutions.					
30287377	8	48	theme	Sips	926:929	arg1	model					931:935	the Sips model	922:935	the Sips model	922:935	The equilibrium data followed the Sips model, with a maximum adsorption capacity of 267.24 mg g-1.					
30287377	2	49	theme	better	333:338	arg1	characteristics					340:354	better characteristics	333:354	better characteristics	333:354	PU/chitosan foam presented better characteristics and adsorption potential than polyurethane foam (PU).					
30287377	10	50	theme	aqueous	1244:1250	arg1	media					1252:1256	aqueous media	1244:1256	aqueous media	1244:1256	The results showed that polyurethane foams are capable to support chitosan, generating an adsorbent with better mechanical characteristics and high potential to remove anionic dyes from aqueous media.					
30112731	0	0	theme	production	68:77	arg1	performance					79:89	production performance	68:89	production performance	68:89	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	5	1	theme	dietary	655:661	arg1	treatments					663:672	The dietary treatments	651:672	The dietary treatments	651:672	The dietary treatments consisted of four proportions of fresh forage ramie (0, 33, 67, and 100%) as a substitute for alfalfa hay (designated as CON, FR33, FR67, and FR100, respectively).					
30112731	9	2	theme	alanine	1362:1368	arg1	ALT					1388:1390	ALT	1388:1390	ALT	1388:1390	Meanwhile, no significant differences were detected in serum parameters between the treatments, with the exception of triglyceride (TG) and alanine aminotransferase (ALT).					
30112731	9	2	theme	alanine	1362:1368	arg1	aminotransferase					1370:1385	alanine aminotransferase	1362:1385	alanine aminotransferase (ALT)	1362:1391	Meanwhile, no significant differences were detected in serum parameters between the treatments, with the exception of triglyceride (TG) and alanine aminotransferase (ALT).					
30112731	2	3	theme	serum	464:468	arg1	parameters					470:479	serum parameters	464:479	serum parameters	464:479	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	5	4	dep	hay	776:778	arg1	designated					781:790	designated	781:790	designated as CON, FR33, FR67, and FR100, respectively	781:834	The dietary treatments consisted of four proportions of fresh forage ramie (0, 33, 67, and 100%) as a substitute for alfalfa hay (designated as CON, FR33, FR67, and FR100, respectively).					
30112731	2	5	theme	dairy	484:488	arg1	cows					490:493	dairy cows	484:493	dairy cows	484:493	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	2	6	theme	Medicago	394:401	arg1	hay					411:413	alfalfa (Medicago sativa) hay	385:413	alfalfa (Medicago sativa) hay	385:413	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	2	6	theme	Medicago	394:401	arg1	sativa					403:408	Medicago sativa	394:408	Medicago sativa	394:408	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	10	7	theme	blood	1581:1585	arg1	parameters					1587:1596	blood parameters	1581:1596	blood parameters	1581:1596	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	2	8	dep	ramie	375:379	arg1	forage					368:373	forage	368:373	forage	368:373	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	6	9	theme	period	876:881	arg1	days					841:844	days 69 and 70	841:854	days	841:844	On days 69 and 70 of the experimental period, milk and blood samples were collected for analysis.					
30112731	10	10	from	diet	1485:1488	arg1	stage					1529:1533	the milk lactation stage	1510:1533	the milk lactation stage	1510:1533	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	10	11	theme	negative	1544:1551	arg1	effects					1553:1559	no negative effects	1541:1559	no negative effects on milk quality and blood parameters	1541:1596	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	7	12	theme	milk	979:982	arg1	yield					984:988	milk yield	979:988	milk yield	979:988	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	10	13	theme	lactation	1519:1527	arg1	stage					1529:1533	the milk lactation stage	1510:1533	the milk lactation stage	1510:1533	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	10	14	from	effects	1553:1559	arg1	parameters					1587:1596	blood parameters	1581:1596	blood parameters	1581:1596	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	10	14	from	effects	1553:1559	arg1	quality					1569:1575	milk quality	1564:1575	milk quality	1564:1575	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	0	15	from	hay	47:49	arg1	diets					54:58	diets	54:58	diets	54:58	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	2	16	theme	cows	490:493	arg1	parameters					470:479	serum parameters	464:479	serum parameters	464:479	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	2	16	theme	cows	490:493	arg1	performance					429:439	production performance	418:439	production performance	418:439	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	2	16	theme	cows	490:493	arg1	composition					447:457	milk composition	442:457	milk composition	442:457	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	7	17	theme	milk	1008:1011	arg1	percentage					1021:1030	milk protein percentage	1008:1030	milk protein percentage	1008:1030	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	7	18	theme	fat	1038:1040	arg1	percentage					1042:1051	milk fat percentage	1033:1051	milk fat percentage	1033:1051	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	2	19	theme	production	418:427	arg1	performance					429:439	production performance	418:439	production performance	418:439	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	1	20	theme	dairy	289:293	arg1	cows					295:298	dairy cows	289:298	dairy cows	289:298	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	7	21	dep	yield	984:988	arg1	percentage					1021:1030	milk protein percentage	1008:1030	milk protein percentage	1008:1030	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	7	21	dep	yield	984:988	arg1	percentage					1071:1080	milk lactose percentage	1058:1080	milk lactose percentage	1058:1080	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	7	21	dep	yield	984:988	arg1	percentage					1042:1051	milk fat percentage	1033:1051	milk fat percentage	1033:1051	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	7	21	dep	yield	984:988	arg1	the					975:977	the	975:977	the	975:977	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	5	22	dep	a	751:751	arg1	substitute					753:762	substitute	753:762	substitute	753:762	The dietary treatments consisted of four proportions of fresh forage ramie (0, 33, 67, and 100%) as a substitute for alfalfa hay (designated as CON, FR33, FR67, and FR100, respectively).					
30112731	6	23	theme	experimental	863:874	arg1	period					876:881	the experimental period	859:881	the experimental period	859:881	On days 69 and 70 of the experimental period, milk and blood samples were collected for analysis.					
30112731	0	24	theme	milk	92:95	arg1	composition					97:107	milk composition	92:107	milk composition	92:107	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	1	25	used	used	224:227	arg2	nivea					184:188	Boehmeria nivea	174:188	Boehmeria nivea (a nettle native to Asia)	174:214	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	1	25	used	used	224:227	arg2	ramie					167:171	ramie	167:171	ramie	167:171	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	1	25	used	used	224:227	arg2	high-quality					234:245	high-quality	234:245	high-quality	234:245	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	10	26	theme	milk	1564:1567	arg1	quality					1569:1575	milk quality	1564:1575	milk quality	1564:1575	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	5	27	theme	fresh	707:711	arg1	%					745:745	0, 33, 67, and 100%	727:745	0, 33, 67, and 100%	727:745	The dietary treatments consisted of four proportions of fresh forage ramie (0, 33, 67, and 100%) as a substitute for alfalfa hay (designated as CON, FR33, FR67, and FR100, respectively).					
30112731	5	27	theme	fresh	707:711	arg1	ramie					720:724	fresh forage ramie	707:724	fresh forage ramie (0, 33, 67, and 100%)	707:746	The dietary treatments consisted of four proportions of fresh forage ramie (0, 33, 67, and 100%) as a substitute for alfalfa hay (designated as CON, FR33, FR67, and FR100, respectively).					
30112731	8	28	dep	ramie	1131:1135	arg1	forage					1124:1129	forage	1124:1129	forage	1124:1129	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	7	29	theme	significant	948:958	arg1	differences					960:970	no significant differences	945:970	no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments	945:1104	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	3	30	theme	cows	604:607	arg1	groups					588:593	four groups	583:593	four groups of eight cows	583:607	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	3	30	theme	cows	604:607	arg1	cows					604:607	eight cows	598:607	eight cows	598:607	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	7	31	theme	milk	994:997	arg1	quality					999:1005	milk quality	994:1005	milk quality	994:1005	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	2	32	theme	fresh	362:366	arg1	ramie					375:379	substituting fresh forage ramie	349:379	substituting fresh forage ramie for alfalfa (Medicago sativa) hay	349:413	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	1	33	theme	cows	295:298	arg1	diets					280:284	diets	280:284	diets of dairy cows	280:298	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	4	34	theme	experimental	614:625	arg1	period					627:632	The experimental period	610:632	The experimental period	610:632	The experimental period lasted 10 weeks.					
30112731	5	35	dep	ramie	720:724	arg1	forage					713:718	forage	713:718	forage	713:718	The dietary treatments consisted of four proportions of fresh forage ramie (0, 33, 67, and 100%) as a substitute for alfalfa hay (designated as CON, FR33, FR67, and FR100, respectively).					
30112731	2	36	theme	substituting	349:360	arg1	ramie					375:379	substituting fresh forage ramie	349:379	substituting fresh forage ramie for alfalfa (Medicago sativa) hay	349:413	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	8	37	theme	matter	1163:1168	arg1	DMI					1178:1180	DMI	1178:1180	DMI	1178:1180	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	8	37	theme	matter	1163:1168	arg1	intake					1170:1175	dry matter intake	1159:1175	dry matter intake (DMI)	1159:1181	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	8	38	theme	dry	1159:1161	arg1	DMI					1178:1180	DMI	1178:1180	DMI	1178:1180	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	8	38	theme	dry	1159:1161	arg1	intake					1170:1175	dry matter intake	1159:1175	dry matter intake (DMI)	1159:1181	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	7	39	theme	lactose	1063:1069	arg1	percentage					1071:1080	milk lactose percentage	1058:1080	milk lactose percentage	1058:1080	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	0	40	theme	fresh	16:20	arg1	ramie					29:33	fresh forage ramie	16:33	fresh forage ramie	16:33	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	8	41	theme	%	1188:1188	arg1	yield					1215:1219	4% fat-corrected milk (FCM) yield	1187:1219	4% fat-corrected milk (FCM) yield	1187:1219	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	7	42	theme	protein	1013:1019	arg1	percentage					1021:1030	milk protein percentage	1008:1030	milk protein percentage	1008:1030	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	10	43	from	ramie	1472:1476	arg1	diet					1485:1488	the diet	1481:1488	the diet of Holstein cows in the milk lactation stage	1481:1533	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	1	44	theme	Boehmeria	174:182	arg1	ramie					167:171	ramie	167:171	ramie	167:171	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	1	44	theme	Boehmeria	174:182	arg1	nivea					184:188	Boehmeria nivea	174:188	Boehmeria nivea (a nettle native to Asia)	174:214	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	10	45	dep	ramie	1472:1476	arg1	forage					1465:1470	forage	1465:1470	forage	1465:1470	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	8	46	theme	4	1187:1187	arg1	%					1188:1188	%	1188:1188	%	1188:1188	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	3	47	theme	Thirty-two	496:505	arg1	 = 590 ± 50.6 kg					538:553	body weight (BW) = 590 ± 50.6 kg	522:553	body weight (BW) = 590 ± 50.6 kg	522:553	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	3	47	theme	Thirty-two	496:505	arg1	cows					516:519	Thirty-two Holstein cows	496:519	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg)	496:554	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	0	48	theme	ramie	29:33	arg1	Substitution					0:11	Substitution	0:11	Substitution of fresh forage ramie for alfalfa hay in diets	0:58	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	0	49	theme	serum	114:118	arg1	parameters					120:129	serum parameters	114:129	serum parameters	114:129	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	8	50	theme	milk	1204:1207	arg1	yield					1215:1219	4% fat-corrected milk (FCM) yield	1187:1219	4% fat-corrected milk (FCM) yield	1187:1219	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	3	51	theme	Holstein	507:514	arg1	 = 590 ± 50.6 kg					538:553	body weight (BW) = 590 ± 50.6 kg	522:553	body weight (BW) = 590 ± 50.6 kg	522:553	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	3	51	theme	Holstein	507:514	arg1	cows					516:519	Thirty-two Holstein cows	496:519	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg)	496:554	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	7	52	theme	milk	1058:1061	arg1	percentage					1071:1080	milk lactose percentage	1058:1080	milk lactose percentage	1058:1080	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	8	53	theme	FCM	1210:1212	arg1	yield					1215:1219	4% fat-corrected milk (FCM) yield	1187:1219	4% fat-corrected milk (FCM) yield	1187:1219	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	0	54	theme	dairy	134:138	arg1	cows					140:143	dairy cows	134:143	dairy cows	134:143	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	8	55	theme	fat-corrected	1190:1202	arg1	yield					1215:1219	4% fat-corrected milk (FCM) yield	1187:1219	4% fat-corrected milk (FCM) yield	1187:1219	We did find that forage ramie significantly reduced dry matter intake (DMI) and 4% fat-corrected milk (FCM) yield.					
30112731	10	56	theme	Holstein	1493:1500	arg1	cows					1502:1505	Holstein cows	1493:1505	Holstein cows	1493:1505	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	1	57	dep	high-quality	234:245	arg1	forage					247:252	forage	247:252	forage	247:252	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	2	58	theme	milk	442:445	arg1	composition					447:457	milk composition	442:457	milk composition	442:457	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	2	59	theme	ramie	375:379	arg1	effects					338:344	the effects	334:344	the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows	334:493	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	0	60	dep	ramie	29:33	arg1	forage					22:27	forage	22:27	forage	22:27	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	9	61	theme	aminotransferase	1370:1385	arg1	exception					1327:1335	the exception	1323:1335	the exception of triglyceride (TG) and alanine aminotransferase (ALT)	1323:1391	Meanwhile, no significant differences were detected in serum parameters between the treatments, with the exception of triglyceride (TG) and alanine aminotransferase (ALT).					
30112731	9	62	theme	serum	1277:1281	arg1	parameters					1283:1292	serum parameters	1277:1292	serum parameters between the treatments	1277:1315	Meanwhile, no significant differences were detected in serum parameters between the treatments, with the exception of triglyceride (TG) and alanine aminotransferase (ALT).					
30112731	9	63	located	detected	1265:1272	arg1	parameters					1283:1292	serum parameters	1277:1292	serum parameters between the treatments	1277:1315	Meanwhile, no significant differences were detected in serum parameters between the treatments, with the exception of triglyceride (TG) and alanine aminotransferase (ALT).					
30112731	9	63	located	detected	1265:1272	arg2	differences					1248:1258	no significant differences	1233:1258	no significant differences	1233:1258	Meanwhile, no significant differences were detected in serum parameters between the treatments, with the exception of triglyceride (TG) and alanine aminotransferase (ALT).					
30112731	3	64	theme	body	522:525	arg1	BW					535:536	BW	535:536	BW	535:536	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	3	64	theme	body	522:525	arg1	weight					527:532	body weight	522:532	body weight (BW) = 590 ± 50.6 kg	522:553	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	1	65	dep	nivea	184:188	arg1	nettle					193:198	a nettle	191:198	a nettle native to Asia	191:213	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	10	66	theme	cows	1502:1505	arg1	diet					1485:1488	the diet	1481:1488	the diet of Holstein cows in the milk lactation stage	1481:1533	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	7	67	theme	milk	1033:1036	arg1	percentage					1042:1051	milk fat percentage	1033:1051	milk fat percentage	1033:1051	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	10	68	theme	alfalfa	1432:1438	arg1	hay					1440:1442	alfalfa hay	1432:1442	alfalfa hay	1432:1442	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	0	69	theme	cows	140:143	arg1	parameters					120:129	serum parameters	114:129	serum parameters	114:129	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	0	69	theme	cows	140:143	arg1	performance					79:89	production performance	68:89	production performance	68:89	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	0	69	theme	cows	140:143	arg1	composition					97:107	milk composition	92:107	milk composition	92:107	Substitution of fresh forage ramie for alfalfa hay in diets affects production performance, milk composition, and serum parameters of dairy cows.					
30112731	3	70	theme	weight	527:532	arg1	 = 590 ± 50.6 kg					538:553	body weight (BW) = 590 ± 50.6 kg	522:553	body weight (BW) = 590 ± 50.6 kg	522:553	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	3	70	theme	weight	527:532	arg1	cows					516:519	Thirty-two Holstein cows	496:519	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg)	496:554	Thirty-two Holstein cows (body weight (BW) = 590 ± 50.6 kg) were randomly divided into four groups of eight cows.					
30112731	6	71	theme	blood	893:897	arg1	samples					899:905	milk and blood samples	884:905	samples	899:905	On days 69 and 70 of the experimental period, milk and blood samples were collected for analysis.					
30112731	10	72	theme	milk	1514:1517	arg1	stage					1529:1533	the milk lactation stage	1510:1533	the milk lactation stage	1510:1533	In conclusion, our data indicate that alfalfa hay can be replaced with forage ramie in the diet of Holstein cows in the milk lactation stage, with no negative effects on milk quality and blood parameters.					
30112731	9	73	theme	significant	1236:1246	arg1	differences					1248:1258	no significant differences	1233:1258	no significant differences	1233:1258	Meanwhile, no significant differences were detected in serum parameters between the treatments, with the exception of triglyceride (TG) and alanine aminotransferase (ALT).					
30112731	7	74	from	differences	960:970	arg1	yield					984:988	milk yield	979:988	milk yield	979:988	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	7	74	from	differences	960:970	arg1	quality					999:1005	milk quality	994:1005	milk quality	994:1005	We found no significant differences in the milk yield and milk quality (milk protein percentage, milk fat percentage, and milk lactose percentage) between the treatments.					
30112731	1	75	theme	native	200:205	arg1	nettle					193:198	a nettle	191:198	a nettle native to Asia	191:213	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	1	76	theme	alfalfa	265:271	arg1	hay					273:275	alfalfa hay	265:275	alfalfa hay	265:275	We hypothesized that ramie, Boehmeria nivea (a nettle native to Asia), can be used as a high-quality forage to replace alfalfa hay in diets of dairy cows.					
30112731	5	77	theme	ramie	720:724	arg1	proportions					692:702	four proportions	687:702	four proportions of fresh forage ramie (0, 33, 67, and 100%)	687:746	The dietary treatments consisted of four proportions of fresh forage ramie (0, 33, 67, and 100%) as a substitute for alfalfa hay (designated as CON, FR33, FR67, and FR100, respectively).					
30112731	2	78	from	effects	338:344	arg1	parameters					470:479	serum parameters	464:479	serum parameters	464:479	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	2	78	from	effects	338:344	arg1	performance					429:439	production performance	418:439	production performance	418:439	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	2	78	from	effects	338:344	arg1	composition					447:457	milk composition	442:457	milk composition	442:457	Accordingly, we aimed to examine the effects of substituting fresh forage ramie for alfalfa (Medicago sativa) hay on production performance, milk composition, and serum parameters of dairy cows.					
30112731	6	79	theme	milk	884:887	arg1	samples					899:905	milk and blood samples	884:905	samples	899:905	On days 69 and 70 of the experimental period, milk and blood samples were collected for analysis.					
30112731	9	80	theme	triglyceride	1340:1351	arg1	exception					1327:1335	the exception	1323:1335	the exception of triglyceride (TG) and alanine aminotransferase (ALT)	1323:1391	Meanwhile, no significant differences were detected in serum parameters between the treatments, with the exception of triglyceride (TG) and alanine aminotransferase (ALT).					
31162876	0	0	theme	anticoagulation	83:97	arg1	regimens					99:106	anticoagulation regimens	83:106	anticoagulation regimens	83:106	Proteins adsorbed to a polysulfone hemodialysis membrane under heparin and citrate anticoagulation regimens.					
31162876	11	1	theme	crosslinked	1646:1656	arg1	formation					1670:1678	imperfectly crosslinked fibrin clot formation	1634:1678	imperfectly crosslinked fibrin clot formation	1634:1678	Compared to heparin, citrate is associated with less protein adsorption and imperfectly crosslinked fibrin clot formation.					
31162876	10	2	theme	growth	1498:1503	arg1	factor					1505:1510	insulin-like growth factor	1485:1510	insulin-like growth factor binding proteins (2, 4, and 5)	1485:1541	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	11	3	link	crosslinked	1646:1656	arg1	formation					1670:1678	imperfectly crosslinked fibrin clot formation	1634:1678	imperfectly crosslinked fibrin clot formation	1634:1678	Compared to heparin, citrate is associated with less protein adsorption and imperfectly crosslinked fibrin clot formation.					
31162876	8	4	with	comparison	1262:1271	arg1	database					1290:1297	the UniProt database	1278:1297	the UniProt database	1278:1297	In these fragments, an analysis of the amino acid sequence has been performed by comparison with the UniProt database.					
31162876	10	5	theme	binding	1512:1518	arg1	proteins					1520:1527	insulin-like growth factor binding proteins	1485:1527	insulin-like growth factor binding proteins (2, 4, and 5)	1485:1541	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	1	6	theme	blood-dialyzer	163:176	arg1	effects					154:160	molecular-level effects	138:160	molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation	138:229	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	1	6	theme	blood-dialyzer	163:176	arg1	interactions					178:189	blood-dialyzer interactions	163:189	blood-dialyzer interactions	163:189	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	11	7	theme	fibrin	1658:1663	arg1	formation					1670:1678	imperfectly crosslinked fibrin clot formation	1634:1678	imperfectly crosslinked fibrin clot formation	1634:1678	Compared to heparin, citrate is associated with less protein adsorption and imperfectly crosslinked fibrin clot formation.					
31162876	7	8	theme	weight	1150:1155	arg1	fragments					1127:1135	fibrin α-chain fragments	1112:1135	fibrin α-chain fragments of molecular weight below 40 kDa	1112:1168	Among the proteins identified as major discriminators between citrate and the other regimens, fibrin α-chain fragments of molecular weight below 40 kDa prevailed.					
31162876	3	9	theme	polysulfone	546:556	arg1	hours					539:543	4 hours	537:543	4 hours	537:543	Following a regular hemodialysis session (4 hours, polysulfone membrane), dialyzers were flushed and the surface biofilm eluted by acetic acid.					
31162876	3	9	theme	polysulfone	546:556	arg1	membrane					558:565	polysulfone membrane	546:565	polysulfone membrane	546:565	Following a regular hemodialysis session (4 hours, polysulfone membrane), dialyzers were flushed and the surface biofilm eluted by acetic acid.					
31162876	0	10	dep	heparin	63:69	arg1	regimens					99:106	anticoagulation regimens	83:106	anticoagulation regimens	83:106	Proteins adsorbed to a polysulfone hemodialysis membrane under heparin and citrate anticoagulation regimens.					
31162876	1	11	theme	dialysis	283:290	arg1	membrane					292:299	dialysis membrane	283:299	dialysis membrane	283:299	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	5	12	theme	responsible	785:795	arg1	Proteins					776:783	Proteins	776:783	Proteins responsible for the difference	776:814	Proteins responsible for the difference were identified by mass spectrometry.					
31162876	10	13	theme	proteins	1425:1432	arg1	cleavage					1389:1396	cleavage	1389:1396	cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin	1389:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	13	theme	proteins	1425:1432	arg1	adsorption					1374:1383	adsorption	1374:1383	adsorption	1374:1383	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	3	14	dep	session	528:534	arg1	hours					539:543	4 hours	537:543	4 hours	537:543	Following a regular hemodialysis session (4 hours, polysulfone membrane), dialyzers were flushed and the surface biofilm eluted by acetic acid.					
31162876	3	14	dep	session	528:534	arg1	membrane					558:565	polysulfone membrane	546:565	polysulfone membrane	546:565	Following a regular hemodialysis session (4 hours, polysulfone membrane), dialyzers were flushed and the surface biofilm eluted by acetic acid.					
31162876	12	15	mod	modified	1730:1737	arg3	regimen					1762:1768	the anticoagulation regimen	1742:1768	the anticoagulation regimen	1742:1768	Membrane adsorptive properties are significantly modified by the anticoagulation regimen.					
31162876	12	15	mod	modified	1730:1737	arg1	properties					1701:1710	Membrane adsorptive properties	1681:1710	Membrane adsorptive properties	1681:1710	Membrane adsorptive properties are significantly modified by the anticoagulation regimen.					
31162876	4	16	theme	resulting	730:738	arg1	patterns					740:747	resulting patterns	730:747	resulting patterns	730:747	Protein composition of the eluates was determined by 2-dimensional gel electrophoresis and resulting patterns compared between regimens.					
31162876	12	17	theme	Membrane	1681:1688	arg1	properties					1701:1710	Membrane adsorptive properties	1681:1710	Membrane adsorptive properties	1681:1710	Membrane adsorptive properties are significantly modified by the anticoagulation regimen.					
31162876	3	18	theme	regular	507:513	arg1	session					528:534	a regular hemodialysis session	505:534	a regular hemodialysis session (4 hours, polysulfone membrane)	505:566	Following a regular hemodialysis session (4 hours, polysulfone membrane), dialyzers were flushed and the surface biofilm eluted by acetic acid.					
31162876	10	19	theme	insulin-like	1485:1496	arg1	factor					1505:1510	insulin-like growth factor	1485:1510	insulin-like growth factor binding proteins (2, 4, and 5)	1485:1541	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	5	20	theme	mass	835:838	arg1	spectrometry					840:851	mass spectrometry	835:851	mass spectrometry	835:851	Proteins responsible for the difference were identified by mass spectrometry.					
31162876	6	21	theme	Citrate	854:860	arg1	anticoagulation					862:876	Citrate anticoagulation	854:876	Citrate anticoagulation	854:876	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	7	22	theme	α-chain	1119:1125	arg1	fragments					1127:1135	fibrin α-chain fragments	1112:1135	fibrin α-chain fragments of molecular weight below 40 kDa	1112:1168	Among the proteins identified as major discriminators between citrate and the other regimens, fibrin α-chain fragments of molecular weight below 40 kDa prevailed.					
31162876	10	23	theme	H-related	1464:1472	arg1	proteins					1425:1432	several heparin-binding proteins	1401:1432	several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin	1401:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	23	theme	H-related	1464:1472	arg1	protein					1474:1480	complement factor H-related protein 3	1446:1482	complement factor H-related protein 3	1446:1482	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	12	24	theme	adsorptive	1690:1699	arg1	properties					1701:1710	Membrane adsorptive properties	1681:1710	Membrane adsorptive properties	1681:1710	Membrane adsorptive properties are significantly modified by the anticoagulation regimen.					
31162876	4	25	theme	gel	706:708	arg1	electrophoresis					710:724	2-dimensional gel electrophoresis	692:724	2-dimensional gel electrophoresis	692:724	Protein composition of the eluates was determined by 2-dimensional gel electrophoresis and resulting patterns compared between regimens.					
31162876	9	26	theme	α-chain	1318:1324	arg1	cross-links					1326:1336	α-chain cross-links	1318:1336	α-chain cross-links	1318:1336	It showed missing α-chain cross-links.					
31162876	10	27	theme	factor	1505:1510	arg1	proteins					1520:1527	insulin-like growth factor binding proteins	1485:1527	insulin-like growth factor binding proteins (2, 4, and 5)	1485:1541	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	28	theme	factor	1457:1462	arg1	proteins					1425:1432	several heparin-binding proteins	1401:1432	several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin	1401:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	28	theme	factor	1457:1462	arg1	protein					1474:1480	complement factor H-related protein 3	1446:1482	complement factor H-related protein 3	1446:1482	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	3	29	theme	surface	600:606	arg1	biofilm					608:614	the surface biofilm	596:614	the surface biofilm	596:614	Following a regular hemodialysis session (4 hours, polysulfone membrane), dialyzers were flushed and the surface biofilm eluted by acetic acid.					
31162876	8	30	theme	UniProt	1282:1288	arg1	database					1290:1297	the UniProt database	1278:1297	the UniProt database	1278:1297	In these fragments, an analysis of the amino acid sequence has been performed by comparison with the UniProt database.					
31162876	1	31	theme	heparin	195:201	arg1	effects					154:160	molecular-level effects	138:160	molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation	138:229	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	1	31	theme	heparin	195:201	arg1	interactions					178:189	blood-dialyzer interactions	163:189	blood-dialyzer interactions	163:189	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	8	32	theme	acid	1226:1229	arg1	sequence					1231:1238	the amino acid sequence	1216:1238	the amino acid sequence	1216:1238	In these fragments, an analysis of the amino acid sequence has been performed by comparison with the UniProt database.					
31162876	10	33	theme	complement	1446:1455	arg1	proteins					1425:1432	several heparin-binding proteins	1401:1432	several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin	1401:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	33	theme	complement	1446:1455	arg1	protein					1474:1480	complement factor H-related protein 3	1446:1482	complement factor H-related protein 3	1446:1482	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	7	34	theme	molecular	1140:1148	arg1	weight					1150:1155	molecular weight	1140:1155	molecular weight below 40 kDa	1140:1168	Among the proteins identified as major discriminators between citrate and the other regimens, fibrin α-chain fragments of molecular weight below 40 kDa prevailed.					
31162876	8	35	theme	amino	1220:1224	arg1	sequence					1231:1238	the amino acid sequence	1216:1238	the amino acid sequence	1216:1238	In these fragments, an analysis of the amino acid sequence has been performed by comparison with the UniProt database.					
31162876	7	36	theme	fibrin	1112:1117	arg1	fragments					1127:1135	fibrin α-chain fragments	1112:1135	fibrin α-chain fragments of molecular weight below 40 kDa	1112:1168	Among the proteins identified as major discriminators between citrate and the other regimens, fibrin α-chain fragments of molecular weight below 40 kDa prevailed.					
31162876	2	37	theme	maintenance	325:335	arg1	hemodialysis					337:348	maintenance hemodialysis	325:348	maintenance hemodialysis	325:348	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	1	38	theme	citrate	207:213	arg1	effects					154:160	molecular-level effects	138:160	molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation	138:229	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	1	38	theme	citrate	207:213	arg1	interactions					178:189	blood-dialyzer interactions	163:189	blood-dialyzer interactions	163:189	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	0	39	theme	hemodialysis	35:46	arg1	membrane					48:55	a polysulfone hemodialysis membrane	21:55	a polysulfone hemodialysis membrane	21:55	Proteins adsorbed to a polysulfone hemodialysis membrane under heparin and citrate anticoagulation regimens.					
31162876	7	40	theme	other	1096:1100	arg1	regimens					1102:1109	the other regimens	1092:1109	the other regimens	1092:1109	Among the proteins identified as major discriminators between citrate and the other regimens, fibrin α-chain fragments of molecular weight below 40 kDa prevailed.					
31162876	4	41	theme	2-dimensional	692:704	arg1	electrophoresis					710:724	2-dimensional gel electrophoresis	692:724	2-dimensional gel electrophoresis	692:724	Protein composition of the eluates was determined by 2-dimensional gel electrophoresis and resulting patterns compared between regimens.					
31162876	0	42	theme	polysulfone	23:33	arg1	membrane					48:55	a polysulfone hemodialysis membrane	21:55	a polysulfone hemodialysis membrane	21:55	Proteins adsorbed to a polysulfone hemodialysis membrane under heparin and citrate anticoagulation regimens.					
31162876	8	43	theme	sequence	1231:1238	arg1	analysis					1204:1211	an analysis	1201:1211	an analysis of the amino acid sequence	1201:1238	In these fragments, an analysis of the amino acid sequence has been performed by comparison with the UniProt database.					
31162876	3	44	theme	acetic	626:631	arg1	acid					633:636	acetic acid	626:636	acetic acid	626:636	Following a regular hemodialysis session (4 hours, polysulfone membrane), dialyzers were flushed and the surface biofilm eluted by acetic acid.					
31162876	7	45	theme	major	1051:1055	arg1	discriminators					1057:1070	major discriminators	1051:1070	major discriminators between citrate and the other regimens	1051:1109	Among the proteins identified as major discriminators between citrate and the other regimens, fibrin α-chain fragments of molecular weight below 40 kDa prevailed.					
31162876	4	46	theme	eluates	666:672	arg1	composition					647:657	Protein composition	639:657	Protein composition of the eluates	639:672	Protein composition of the eluates was determined by 2-dimensional gel electrophoresis and resulting patterns compared between regimens.					
31162876	12	47	theme	anticoagulation	1746:1760	arg1	regimen					1762:1768	the anticoagulation regimen	1742:1768	the anticoagulation regimen	1742:1768	Membrane adsorptive properties are significantly modified by the anticoagulation regimen.					
31162876	10	48	dep	proteins	1520:1527	arg1	4					1533:1533	4	1533:1533	4	1533:1533	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	48	dep	proteins	1520:1527	arg1	5					1540:1540	5	1540:1540	5	1540:1540	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	6	49	theme	protein	917:923	arg1	adsorption					925:934	significantly less protein adsorption	898:934	significantly less protein adsorption to the membrane than heparin	898:963	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	2	50	theme	crossover	369:377	arg1	design					379:384	a crossover design	367:384	a crossover design	367:384	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	6	51	dep	associated	882:891	arg1	mg					980:981	mg	980:981	mg	980:981	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	6	51	dep	associated	882:891	arg1	P					1006:1006	P	1006:1006	P	1006:1006	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	6	51	dep	associated	882:891	arg1	=					1008:1008	=	1008:1008	=	1008:1008	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	6	51	dep	associated	882:891	arg1	0.009					1010:1014	0.009	1010:1014	0.009	1010:1014	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	6	51	dep	associated	882:891	arg1	2.2					966:968	2.2	966:968	2.2	966:968	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	6	51	dep	associated	882:891	arg1	[2.9-11.6					991:999	[2.9-11.6	991:999	[2.9-11.6	991:999	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	6	51	dep	associated	882:891	arg1	[1.1-2.9					970:977	[1.1-2.9	970:977	[1.1-2.9	970:977	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	1	52	theme	molecular-level	138:152	arg1	effects					154:160	molecular-level effects	138:160	molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation	138:229	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	1	52	theme	molecular-level	138:152	arg1	interactions					178:189	blood-dialyzer interactions	163:189	blood-dialyzer interactions	163:189	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	1	53	theme	study	113:117	arg1	aim					119:121	The study aim	109:121	The study aim	109:121	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	1	54	dep	heparin	195:201	arg1	anticoagulation					215:229	anticoagulation	215:229	anticoagulation	215:229	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	3	55	theme	hemodialysis	515:526	arg1	session					528:534	a regular hemodialysis session	505:534	a regular hemodialysis session (4 hours, polysulfone membrane)	505:566	Following a regular hemodialysis session (4 hours, polysulfone membrane), dialyzers were flushed and the surface biofilm eluted by acetic acid.					
31162876	1	56	theme	proteome-wide	237:249	arg1	analysis					251:258	proteome-wide analysis	237:258	proteome-wide analysis of biofilm	237:269	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	6	57	theme	less	912:915	arg1	adsorption					925:934	significantly less protein adsorption	898:934	significantly less protein adsorption to the membrane than heparin	898:963	Citrate anticoagulation was associated with significantly less protein adsorption to the membrane than heparin (2.2 [1.1-2.9] mg vs. 6.5 [2.9-11.6] mg, P = 0.009).					
31162876	1	58	dep	using	231:235	arg1	adsorbed					271:278	adsorbed	271:278	using proteome-wide analysis of biofilm adsorbed to dialysis membrane	231:299	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
31162876	10	59	theme	several	1401:1407	arg1	chemerin					1548:1555	chemerin	1548:1555	chemerin	1548:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	59	theme	several	1401:1407	arg1	proteins					1425:1432	several heparin-binding proteins	1401:1432	several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin	1401:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	59	theme	several	1401:1407	arg1	protein					1474:1480	complement factor H-related protein 3	1446:1482	complement factor H-related protein 3	1446:1482	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	11	60	theme	imperfectly	1634:1644	arg1	formation					1670:1678	imperfectly crosslinked fibrin clot formation	1634:1678	imperfectly crosslinked fibrin clot formation	1634:1678	Compared to heparin, citrate is associated with less protein adsorption and imperfectly crosslinked fibrin clot formation.					
31162876	2	61	theme	anticoagulation	408:422	arg1	heparin					450:456	heparin	450:456	heparin	450:456	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	2	61	theme	anticoagulation	408:422	arg1	regimens					424:431	three different anticoagulation regimens	392:431	three different anticoagulation regimens	392:431	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	2	61	theme	anticoagulation	408:422	arg1	citrate					441:447	citrate	441:447	citrate	441:447	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	2	61	theme	anticoagulation	408:422	arg1	anticoagulation-free					463:482	anticoagulation-free	463:482	anticoagulation-free (control)	463:492	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	10	62	theme	heparin-binding	1409:1423	arg1	chemerin					1548:1555	chemerin	1548:1555	chemerin	1548:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	62	theme	heparin-binding	1409:1423	arg1	proteins					1425:1432	several heparin-binding proteins	1401:1432	several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin	1401:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	62	theme	heparin-binding	1409:1423	arg1	protein					1474:1480	complement factor H-related protein 3	1446:1482	complement factor H-related protein 3	1446:1482	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	11	63	theme	clot	1665:1668	arg1	formation					1670:1678	imperfectly crosslinked fibrin clot formation	1634:1678	imperfectly crosslinked fibrin clot formation	1634:1678	Compared to heparin, citrate is associated with less protein adsorption and imperfectly crosslinked fibrin clot formation.					
31162876	4	64	theme	Protein	639:645	arg1	composition					647:657	Protein composition	639:657	Protein composition of the eluates	639:672	Protein composition of the eluates was determined by 2-dimensional gel electrophoresis and resulting patterns compared between regimens.					
31162876	11	65	theme	protein	1611:1617	arg1	adsorption					1619:1628	less protein adsorption	1606:1628	less protein adsorption	1606:1628	Compared to heparin, citrate is associated with less protein adsorption and imperfectly crosslinked fibrin clot formation.					
31162876	2	66	theme	different	398:406	arg1	heparin					450:456	heparin	450:456	heparin	450:456	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	2	66	theme	different	398:406	arg1	regimens					424:431	three different anticoagulation regimens	392:431	three different anticoagulation regimens	392:431	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	2	66	theme	different	398:406	arg1	citrate					441:447	citrate	441:447	citrate	441:447	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	2	66	theme	different	398:406	arg1	anticoagulation-free					463:482	anticoagulation-free	463:482	anticoagulation-free (control)	463:492	Ten patients receiving maintenance hemodialysis were examined in a crossover design under three different anticoagulation regimens, namely citrate, heparin, and anticoagulation-free (control).					
31162876	10	67	dep	proteins	1425:1432	arg1	proteins					1425:1432	several heparin-binding proteins	1401:1432	several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin	1401:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	67	dep	proteins	1425:1432	arg1	proteins					1520:1527	insulin-like growth factor binding proteins	1485:1527	insulin-like growth factor binding proteins (2, 4, and 5)	1485:1541	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	67	dep	proteins	1425:1432	arg1	chemerin					1548:1555	chemerin	1548:1555	chemerin	1548:1555	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	10	67	dep	proteins	1425:1432	arg1	protein					1474:1480	complement factor H-related protein 3	1446:1482	complement factor H-related protein 3	1446:1482	On the contrary, heparin prevented adsorption and cleavage of several heparin-binding proteins; especially complement factor H-related protein 3, insulin-like growth factor binding proteins (2, 4, and 5), and chemerin.					
31162876	1	68	theme	biofilm	263:269	arg1	analysis					251:258	proteome-wide analysis	237:258	proteome-wide analysis of biofilm	237:269	The study aim was to compare molecular-level effects (blood-dialyzer interactions) of heparin and citrate anticoagulation using proteome-wide analysis of biofilm adsorbed to dialysis membrane.					
30925992	7	0	theme	PPY	1152:1154	arg1	HA					1156:1157	DTX/PPN@PPY@HA	1144:1157	DTX/PPN@PPY@HA against tumor growth	1144:1178	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	6	1	theme	@	966:966	arg1	HA					967:968	DTX/PPN@PPY@HA	955:968	DTX/PPN@PPY@HA	955:968	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	4	2	theme	@	659:659	arg1	agent					702:706	an effective photoacoustic contrast agent	666:706	an effective photoacoustic contrast agent	666:706	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	2	theme	@	659:659	arg1	HA					660:661	DTX/PPN@PPY@HA	648:661	DTX/PPN@PPY@HA	648:661	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	1	3	theme	photothermal	192:203	arg1	effect					205:210	photothermal effect	192:210	photothermal effect	192:210	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	6	4	theme	@	962:962	arg1	HA					967:968	DTX/PPN@PPY@HA	955:968	DTX/PPN@PPY@HA	955:968	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	7	5	theme	@	1155:1155	arg1	HA					1156:1157	DTX/PPN@PPY@HA	1144:1157	DTX/PPN@PPY@HA against tumor growth	1144:1178	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	4	6	theme	chemotherapy	808:819	arg1	region					762:767	tumor region	756:767	tumor region	756:767	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	6	theme	chemotherapy	808:819	arg1	guidance					783:790	real-time guidance	773:790	real-time guidance of photothermal chemotherapy	773:819	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	1	7	theme	effect	205:210	arg1	advantages					178:187	the combined advantages	165:187	the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging	165:297	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	6	8	theme	DTX/PPN	955:961	arg1	HA					967:968	DTX/PPN@PPY@HA	955:968	DTX/PPN@PPY@HA	955:968	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	4	9	theme	real-time	773:781	arg1	guidance					783:790	real-time guidance	773:790	real-time guidance of photothermal chemotherapy	773:819	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	2	10	theme	outer	377:381	arg1	material					357:364	the phase change material	340:364	the phase change material (PCM)	340:370	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	10	theme	outer	377:381	arg1	shell					401:405	the outer polypyrrole (PPY) shell	373:405	the outer polypyrrole (PPY) shell	373:405	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	3	11	theme	DTX/PPN	512:518	arg1	HA					524:525	denoted as DTX/PPN@PPY@HA	501:525	denoted as DTX/PPN@PPY@HA	501:525	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	11	theme	DTX/PPN	512:518	arg1	nanoparticles					486:498	The obtained composite nanoparticles	463:498	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA)	463:526	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	2	12	theme	phase	344:348	arg1	acid					426:429	the hyaluronic acid	411:429	the hyaluronic acid (HA)	411:434	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	12	theme	phase	344:348	arg1	PCM					367:369	PCM	367:369	PCM	367:369	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	12	theme	phase	344:348	arg1	material					357:364	the phase change material	340:364	the phase change material (PCM)	340:370	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	12	theme	phase	344:348	arg1	shell					401:405	the outer polypyrrole (PPY) shell	373:405	the outer polypyrrole (PPY) shell	373:405	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	5	13	theme	drug	895:898	arg1	release					900:906	temperature-sensitive drug release	873:906	temperature-sensitive drug release	873:906	DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release.					
30925992	6	14	with	cytotoxicity	1045:1056	arg1	the					1063:1065	the	1063:1065	the	1063:1065	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	4	15	dep	in	595:596	arg1	vitro					598:602	vitro	598:602	vitro	598:602	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	2	16	theme	polypyrrole	383:393	arg1	material					357:364	the phase change material	340:364	the phase change material (PCM)	340:370	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	16	theme	polypyrrole	383:393	arg1	shell					401:405	the outer polypyrrole (PPY) shell	373:405	the outer polypyrrole (PPY) shell	373:405	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	3	17	theme	composite	476:484	arg1	nanoparticles					486:498	The obtained composite nanoparticles	463:498	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA)	463:526	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	17	theme	composite	476:484	arg1	HA					524:525	denoted as DTX/PPN@PPY@HA	501:525	denoted as DTX/PPN@PPY@HA	501:525	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	17	theme	composite	476:484	arg1	spherical					533:541	spherical	533:541	spherical	533:541	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	4	18	theme	PPY	656:658	arg1	agent					702:706	an effective photoacoustic contrast agent	666:706	an effective photoacoustic contrast agent	666:706	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	18	theme	PPY	656:658	arg1	HA					660:661	DTX/PPN@PPY@HA	648:661	DTX/PPN@PPY@HA	648:661	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	7	19	theme	tumor-bearing	1200:1212	arg1	model					1219:1223	4T1 tumor-bearing mice model	1196:1223	4T1 tumor-bearing mice model	1196:1223	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	5	20	theme	DTX/PPN	822:828	arg1	HA					834:835	DTX/PPN@PPY@HA	822:835	DTX/PPN@PPY@HA	822:835	DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release.					
30925992	3	21	theme	PPY	520:522	arg1	HA					524:525	denoted as DTX/PPN@PPY@HA	501:525	denoted as DTX/PPN@PPY@HA	501:525	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	21	theme	PPY	520:522	arg1	nanoparticles					486:498	The obtained composite nanoparticles	463:498	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA)	463:526	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	5	22	theme	@	829:829	arg1	HA					834:835	DTX/PPN@PPY@HA	822:835	DTX/PPN@PPY@HA	822:835	DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release.					
30925992	6	23	dep	the	1063:1065	arg1	help					1067:1070	help	1067:1070	help	1067:1070	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	0	24	theme	synergistic	71:81	arg1	therapy					102:108	synergistic chemo-photothermal therapy	71:108	synergistic chemo-photothermal therapy	71:108	Novel hyaluronic acid-modified temperature-sensitive nanoparticles for synergistic chemo-photothermal therapy.					
30925992	5	25	theme	good	843:846	arg1	effects					861:867	good photothermal effects	843:867	good photothermal effects	843:867	DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release.					
30925992	4	26	theme	guidance	783:790	arg1	localization					740:751	accurate localization	731:751	accurate localization of tumor region and real-time guidance of photothermal chemotherapy	731:819	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	7	27	theme	DTX/PPN	1144:1150	arg1	HA					1156:1157	DTX/PPN@PPY@HA	1144:1157	DTX/PPN@PPY@HA against tumor growth	1144:1178	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	3	28	theme	@	523:523	arg1	HA					524:525	denoted as DTX/PPN@PPY@HA	501:525	denoted as DTX/PPN@PPY@HA	501:525	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	28	theme	@	523:523	arg1	nanoparticles					486:498	The obtained composite nanoparticles	463:498	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA)	463:526	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	6	29	theme	significant	1033:1043	arg1	cytotoxicity					1045:1056	significant cytotoxicity	1033:1056	significant cytotoxicity with the help of near-infrared (NIR) laser	1033:1099	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	1	30	theme	active	213:218	arg1	tumor-targeting					220:234	active tumor-targeting	213:234	active tumor-targeting	213:234	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	1	31	with	nano-system	148:158	arg1	advantages					178:187	the combined advantages	165:187	the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging	165:297	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	4	32	theme	contrast	693:700	arg1	agent					702:706	an effective photoacoustic contrast agent	666:706	an effective photoacoustic contrast agent	666:706	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	32	theme	contrast	693:700	arg1	HA					660:661	DTX/PPN@PPY@HA	648:661	DTX/PPN@PPY@HA	648:661	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	7	33	theme	4T1	1196:1198	arg1	model					1219:1223	4T1 tumor-bearing mice model	1196:1223	4T1 tumor-bearing mice model	1196:1223	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	1	34	theme	tumor-targeting	220:234	arg1	advantages					178:187	the combined advantages	165:187	the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging	165:297	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	3	35	with	spherical	533:541	arg1	diameter					555:562	a mean diameter	548:562	a mean diameter of about 232.7 nm	548:580	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	4	36	theme	photothermal	795:806	arg1	chemotherapy					808:819	photothermal chemotherapy	795:819	photothermal chemotherapy	795:819	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	2	37	theme	material	357:364	arg1	core					332:335	the core	328:335	the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell	328:460	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	5	38	theme	PPY	830:832	arg1	HA					834:835	DTX/PPN@PPY@HA	822:835	DTX/PPN@PPY@HA	822:835	DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release.					
30925992	7	39	theme	@	1151:1151	arg1	HA					1156:1157	DTX/PPN@PPY@HA	1144:1157	DTX/PPN@PPY@HA against tumor growth	1144:1178	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	6	40	theme	cellular	922:929	arg1	experiments					931:941	cellular experiments	922:941	cellular experiments	922:941	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	5	41	theme	temperature-sensitive	873:893	arg1	release					900:906	temperature-sensitive drug release	873:906	temperature-sensitive drug release	873:906	DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release.					
30925992	2	42	theme	change	350:355	arg1	acid					426:429	the hyaluronic acid	411:429	the hyaluronic acid (HA)	411:434	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	42	theme	change	350:355	arg1	PCM					367:369	PCM	367:369	PCM	367:369	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	42	theme	change	350:355	arg1	material					357:364	the phase change material	340:364	the phase change material (PCM)	340:370	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	42	theme	change	350:355	arg1	shell					401:405	the outer polypyrrole (PPY) shell	373:405	the outer polypyrrole (PPY) shell	373:405	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	1	43	theme	temperature-sensitive	237:257	arg1	release					264:270	temperature-sensitive drug release	237:270	temperature-sensitive drug release	237:270	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	4	44	theme	photoacoustic	679:691	arg1	agent					702:706	an effective photoacoustic contrast agent	666:706	an effective photoacoustic contrast agent	666:706	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	44	theme	photoacoustic	679:691	arg1	HA					660:661	DTX/PPN@PPY@HA	648:661	DTX/PPN@PPY@HA	648:661	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	1	45	theme	versatile	138:146	arg1	nano-system					148:158	a versatile nano-system	136:158	a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging	136:297	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	1	46	theme	drug	259:262	arg1	release					264:270	temperature-sensitive drug release	237:270	temperature-sensitive drug release	237:270	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	3	47	theme	denoted	501:507	arg1	HA					524:525	denoted as DTX/PPN@PPY@HA	501:525	denoted as DTX/PPN@PPY@HA	501:525	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	47	theme	denoted	501:507	arg1	nanoparticles					486:498	The obtained composite nanoparticles	463:498	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA)	463:526	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	6	48	theme	near-infrared	1075:1087	arg1	laser					1095:1099	near-infrared (NIR) laser	1075:1099	near-infrared (NIR) laser	1075:1099	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	6	48	theme	near-infrared	1075:1087	arg1	NIR					1090:1092	NIR	1090:1092	NIR	1090:1092	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	4	49	theme	photoacoustic	604:616	arg1	experiments					626:636	In vivo and in vitro photoacoustic imaging experiments	583:636	In vivo and in vitro photoacoustic imaging experiments	583:636	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	50	theme	region	762:767	arg1	localization					740:751	accurate localization	731:751	accurate localization of tumor region and real-time guidance of photothermal chemotherapy	731:819	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	6	51	theme	tumor	1009:1013	arg1	cells					1015:1019	tumor cells	1009:1019	tumor cells	1009:1019	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	1	52	theme	release	264:270	arg1	advantages					178:187	the combined advantages	165:187	the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging	165:297	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	4	53	theme	@	655:655	arg1	agent					702:706	an effective photoacoustic contrast agent	666:706	an effective photoacoustic contrast agent	666:706	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	53	theme	@	655:655	arg1	HA					660:661	DTX/PPN@PPY@HA	648:661	DTX/PPN@PPY@HA	648:661	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	54	theme	tumor	756:760	arg1	region					762:767	tumor region	756:767	tumor region	756:767	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	2	55	theme	hyaluronic	415:424	arg1	acid					426:429	the hyaluronic acid	411:429	the hyaluronic acid (HA)	411:434	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	55	theme	hyaluronic	415:424	arg1	material					357:364	the phase change material	340:364	the phase change material (PCM)	340:370	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	55	theme	hyaluronic	415:424	arg1	HA					432:433	HA	432:433	HA	432:433	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	0	56	theme	chemo-photothermal	83:100	arg1	therapy					102:108	synergistic chemo-photothermal therapy	71:108	synergistic chemo-photothermal therapy	71:108	Novel hyaluronic acid-modified temperature-sensitive nanoparticles for synergistic chemo-photothermal therapy.					
30925992	5	57	theme	@	833:833	arg1	HA					834:835	DTX/PPN@PPY@HA	822:835	DTX/PPN@PPY@HA	822:835	DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release.					
30925992	3	58	theme	mean	550:553	arg1	diameter					555:562	a mean diameter	548:562	a mean diameter of about 232.7 nm	548:580	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	5	59	theme	photothermal	848:859	arg1	effects					861:867	good photothermal effects	843:867	good photothermal effects	843:867	DTX/PPN@PPY@HA shows good photothermal effects and temperature-sensitive drug release.					
30925992	2	60	theme	PPY	452:454	arg1	shell					456:460	the PPY shell	448:460	the PPY shell	448:460	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	3	61	theme	as	509:510	arg1	HA					524:525	denoted as DTX/PPN@PPY@HA	501:525	denoted as DTX/PPN@PPY@HA	501:525	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	61	theme	as	509:510	arg1	nanoparticles					486:498	The obtained composite nanoparticles	463:498	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA)	463:526	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	4	62	theme	effective	669:677	arg1	agent					702:706	an effective photoacoustic contrast agent	666:706	an effective photoacoustic contrast agent	666:706	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	62	theme	effective	669:677	arg1	HA					660:661	DTX/PPN@PPY@HA	648:661	DTX/PPN@PPY@HA	648:661	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	7	63	theme	remarkable	1119:1128	arg1	inhibition					1130:1139	the remarkable inhibition	1115:1139	the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth	1115:1178	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	4	64	dep	In	583:584	arg1	vivo					586:589	vivo	586:589	vivo	586:589	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	65	theme	accurate	731:738	arg1	localization					740:751	accurate localization	731:751	accurate localization of tumor region and real-time guidance of photothermal chemotherapy	731:819	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	66	theme	DTX/PPN	648:654	arg1	agent					702:706	an effective photoacoustic contrast agent	666:706	an effective photoacoustic contrast agent	666:706	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	66	theme	DTX/PPN	648:654	arg1	HA					660:661	DTX/PPN@PPY@HA	648:661	DTX/PPN@PPY@HA	648:661	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	3	67	theme	obtained	467:474	arg1	nanoparticles					486:498	The obtained composite nanoparticles	463:498	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA)	463:526	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	67	theme	obtained	467:474	arg1	HA					524:525	denoted as DTX/PPN@PPY@HA	501:525	denoted as DTX/PPN@PPY@HA	501:525	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	67	theme	obtained	467:474	arg1	spherical					533:541	spherical	533:541	spherical	533:541	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	4	68	used	used	722:725	arg2	HA					660:661	DTX/PPN@PPY@HA	648:661	DTX/PPN@PPY@HA	648:661	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	4	68	used	used	722:725	arg2	agent					702:706	an effective photoacoustic contrast agent	666:706	an effective photoacoustic contrast agent	666:706	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	7	69	theme	mice	1214:1217	arg1	model					1219:1223	4T1 tumor-bearing mice model	1196:1223	4T1 tumor-bearing mice model	1196:1223	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	1	70	theme	combined	169:176	arg1	advantages					178:187	the combined advantages	165:187	the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging	165:297	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	1	71	theme	photoacoustic	277:289	arg1	imaging					291:297	photoacoustic imaging	277:297	photoacoustic imaging	277:297	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	7	72	theme	HA	1156:1157	arg1	inhibition					1130:1139	the remarkable inhibition	1115:1139	the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth	1115:1178	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	3	73	theme	232.7 nm	573:580	arg1	diameter					555:562	a mean diameter	548:562	a mean diameter of about 232.7 nm	548:580	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	4	74	theme	in	595:596	arg1	experiments					626:636	In vivo and in vitro photoacoustic imaging experiments	583:636	In vivo and in vitro photoacoustic imaging experiments	583:636	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	2	75	theme	PPY	396:398	arg1	material					357:364	the phase change material	340:364	the phase change material (PCM)	340:370	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	2	75	theme	PPY	396:398	arg1	shell					401:405	the outer polypyrrole (PPY) shell	373:405	the outer polypyrrole (PPY) shell	373:405	The nano-system consists of the core of the phase change material (PCM), the outer polypyrrole (PPY) shell and the hyaluronic acid (HA) modified in the PPY shell.					
30925992	4	76	theme	imaging	618:624	arg1	experiments					626:636	In vivo and in vitro photoacoustic imaging experiments	583:636	In vivo and in vitro photoacoustic imaging experiments	583:636	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
30925992	1	77	theme	imaging	291:297	arg1	advantages					178:187	the combined advantages	165:187	the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging	165:297	This study has developed a versatile nano-system with the combined advantages of photothermal effect, active tumor-targeting, temperature-sensitive drug release, and photoacoustic imaging.					
30925992	6	78	theme	PPY	963:965	arg1	HA					967:968	DTX/PPN@PPY@HA	955:968	DTX/PPN@PPY@HA	955:968	In addition, cellular experiments showed that DTX/PPN@PPY@HA could be efficiently internalized into tumor cells and produce significant cytotoxicity with the help of near-infrared (NIR) laser.					
30925992	3	79	theme	@	519:519	arg1	HA					524:525	denoted as DTX/PPN@PPY@HA	501:525	denoted as DTX/PPN@PPY@HA	501:525	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	3	79	theme	@	519:519	arg1	nanoparticles					486:498	The obtained composite nanoparticles	463:498	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA)	463:526	The obtained composite nanoparticles (denoted as DTX/PPN@PPY@HA) were spherical with a mean diameter of about 232.7 nm.					
30925992	7	80	theme	tumor	1167:1171	arg1	growth					1173:1178	tumor growth	1167:1178	tumor growth	1167:1178	Furthermore, the remarkable inhibition of DTX/PPN@PPY@HA against tumor growth was achieved in 4T1 tumor-bearing mice model.					
30925992	4	81	theme	In	583:584	arg1	experiments					626:636	In vivo and in vitro photoacoustic imaging experiments	583:636	In vivo and in vitro photoacoustic imaging experiments	583:636	In vivo and in vitro photoacoustic imaging experiments show that DTX/PPN@PPY@HA is an effective photoacoustic contrast agent, which can be used for accurate localization of tumor region and real-time guidance of photothermal chemotherapy.					
29297964	4	0	theme	in vitro	819:826	arg1	IVDMD					854:858	IVDMD	854:858	IVDMD	854:858	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	4	0	theme	in vitro	819:826	arg1	digestibility					839:851	in vitro dry matter digestibility	819:851	in vitro dry matter digestibility (IVDMD)	819:859	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	3	1	theme	day/3°C	647:653	arg1	warming					631:637	infrared warming	622:637	infrared warming (+1.5°C day/3°C night)	622:660	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	3	1	theme	day/3°C	647:653	arg1	night					655:659	+1.5°C day/3°C night	640:659	+1.5°C day/3°C night	640:659	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	7	2	theme	grass	1316:1320	arg1	tissues					1322:1328	grass tissues	1316:1328	grass tissues	1316:1328	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	4	3	theme	matter	832:837	arg1	IVDMD					854:858	IVDMD	854:858	IVDMD	854:858	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	4	3	theme	matter	832:837	arg1	digestibility					839:851	in vitro dry matter digestibility	819:851	in vitro dry matter digestibility (IVDMD)	819:859	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	7	4	theme	detergent	1283:1291	arg1	content					1305:1311	acid detergent fiber (ADF) content	1278:1311	acid detergent fiber (ADF) content of grass tissues	1278:1328	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	1	5	from	herbivores	309:318	arg1	ecosystems					333:342	rangeland ecosystems	323:342	rangeland ecosystems	323:342	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	9	6	dep	%	1699:1699	arg1	to					1692:1693	to	1692:1693	to	1692:1693	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	3	7	theme	realistic	668:676	arg1	conditions					684:693	realistic field conditions	668:693	realistic field conditions	668:693	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	8	8	theme	relative	1510:1517	arg1	%					1508:1508	13%	1506:1508	13% relative to ambient climate	1506:1536	For the six most abundant forage species (representing 96% of total forage production), combined warming and eCO2 increased forage production by 38% and reduced forage [N] by 13% relative to ambient climate.					
29297964	7	9	theme	ADF	1300:1302	arg1	content					1305:1311	acid detergent fiber (ADF) content	1278:1311	acid detergent fiber (ADF) content of grass tissues	1278:1328	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	9	10	theme	primary	1833:1839	arg1	season					1849:1854	the primary growing season	1829:1854	the primary growing season in the largest remaining rangeland ecosystem in North America	1829:1916	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	8	11	theme	ambient	1522:1528	arg1	climate					1530:1536	ambient climate	1522:1536	ambient climate	1522:1536	For the six most abundant forage species (representing 96% of total forage production), combined warming and eCO2 increased forage production by 38% and reduced forage [N] by 13% relative to ambient climate.					
29297964	4	12	theme	tissue	865:870	arg1	[N					872:873	tissue [N	865:873	tissue [N	865:873	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	7	13	theme	reduced	1252:1258	arg1	[N					1260:1261	reduced [N	1252:1261	reduced [N	1252:1261	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	6	14	theme	effects	1199:1205	arg1	independent					1176:1186	independent	1176:1186	independent	1176:1186	Furthermore, eCO2 reduced IVDMD and [N] independent of warming effects.					
29297964	1	15	theme	Increasing	122:131	arg1	[CO2					145:148	Increasing atmospheric [CO2 ]	122:150	Increasing atmospheric [CO2 ]	122:150	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	10	16	from	rates	2122:2126	arg1	interseeding					2140:2151	legume interseeding	2133:2151	legume interseeding	2133:2151	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	10	16	from	rates	2122:2126	arg1	fertilization					2101:2113	fertilization	2101:2113	fertilization at low rates	2101:2126	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	10	16	from	rates	2122:2126	arg1	burning					2092:2098	prescribed burning	2081:2098	prescribed burning	2081:2098	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	10	17	theme	low	2118:2120	arg1	rates					2122:2126	low rates	2118:2126	low rates	2118:2126	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	0	18	from	warming	91:97	arg1	prairie					113:119	mixedgrass prairie	102:119	mixedgrass prairie	102:119	Elevated CO2 induces substantial and persistent declines in forage quality irrespective of warming in mixedgrass prairie.					
29297964	7	19	theme	Reduced	1208:1214	arg1	IVDMD					1216:1220	Reduced IVDMD	1208:1220	Reduced IVDMD with eCO2	1208:1230	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	4	20	located	detected	881:888	arg1	years					906:910	certain years	898:910	certain years	898:910	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	4	20	located	detected	881:888	arg2	[N					872:873	tissue [N	865:873	tissue [N	865:873	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	4	20	located	detected	881:888	arg2	effects					808:814	warming effects	800:814	warming effects on in vitro dry matter digestibility (IVDMD)	800:859	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	3	21	theme	mixedgrass	741:750	arg1	semiarid					731:738	a semiarid	729:738	a semiarid	729:738	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	3	21	theme	mixedgrass	741:750	arg1	prairie					752:758	mixedgrass prairie	741:758	mixedgrass prairie	741:758	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	7	22	with	IVDMD	1216:1220	arg1	eCO2					1227:1230	eCO2	1227:1230	eCO2	1227:1230	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	5	23	theme	abundant	1030:1037	arg1	grasses					1039:1045	two most abundant grasses	1021:1045	two most abundant grasses	1021:1045	In contrast, eCO2 substantially reduced IVDMD (two most abundant grasses) and [N] (all three dominant grass species) in most years, except the two wettest years.					
29297964	5	23	theme	abundant	1030:1037	arg1	IVDMD					1014:1018	IVDMD	1014:1018	IVDMD (two most abundant grasses)	1014:1046	In contrast, eCO2 substantially reduced IVDMD (two most abundant grasses) and [N] (all three dominant grass species) in most years, except the two wettest years.					
29297964	3	24	theme	CO2	590:592	arg1	enrichment					594:603	CO2 enrichment	590:603	CO2 enrichment (to 600 ppm)	590:616	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	3	24	theme	CO2	590:592	arg1	600 ppm					609:615	600 ppm	609:615	600 ppm	609:615	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	9	25	dep	%	1675:1675	arg1	to					1668:1669	to	1668:1669	to	1668:1669	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	9	25	dep	%	1675:1675	arg1	IVDMD					1677:1681	IVDMD	1677:1681	63.3 to 61.1% IVDMD	1663:1681	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	7	26	dep	[N	1260:1261	arg1	both					1244:1247	both	1244:1247	both	1244:1247	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	9	27	theme	absolute	1552:1559	arg1	magnitude					1561:1569	the absolute magnitude	1548:1569	the absolute magnitude of the decline in IVDMD	1548:1593	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	1	28	theme	forage	289:294	arg1	quality					278:284	quality	278:284	quality	278:284	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	1	28	theme	forage	289:294	arg1	quantity					265:272	quantity	265:272	quantity	265:272	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	1	28	theme	forage	289:294	arg1	biogeochemistry					230:244	biogeochemistry	230:244	biogeochemistry	230:244	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	1	28	theme	forage	289:294	arg1	productivity					195:206	the productivity	191:206	the productivity	191:206	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	1	28	theme	forage	289:294	arg1	composition					217:227	species composition	209:227	species composition	209:227	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	0	29	theme	irrespective	75:86	arg1	quality					67:73	quality	67:73	quality irrespective of warming in mixedgrass prairie	67:119	Elevated CO2 induces substantial and persistent declines in forage quality irrespective of warming in mixedgrass prairie.					
29297964	10	30	theme	legume	2133:2138	arg1	interseeding					2140:2151	legume interseeding	2133:2151	legume interseeding	2133:2151	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	9	31	contain	have	1748:1751	arg2	consequences					1765:1776	substantial consequences	1753:1776	substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America	1753:1916	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	9	31	contain	have	1748:1751	arg1	shifts					1735:1740	such shifts	1730:1740	such shifts	1730:1740	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	0	32	theme	warming	91:97	arg1	irrespective					75:86	irrespective	75:86	irrespective	75:86	Elevated CO2 induces substantial and persistent declines in forage quality irrespective of warming in mixedgrass prairie.					
29297964	4	33	from	effects	808:814	arg1	IVDMD					854:858	IVDMD	854:858	IVDMD	854:858	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	4	33	from	effects	808:814	arg1	digestibility					839:851	in vitro dry matter digestibility	819:851	in vitro dry matter digestibility (IVDMD)	819:859	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	9	34	theme	decline	1578:1584	arg1	magnitude					1561:1569	the absolute magnitude	1548:1569	the absolute magnitude of the decline in IVDMD	1548:1593	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	2	35	theme	plant	390:394	arg1	chemistry					403:411	plant tissue chemistry	390:411	plant tissue chemistry	390:411	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	0	36	theme	mixedgrass	102:111	arg1	prairie					113:119	mixedgrass prairie	102:119	mixedgrass prairie	102:119	Elevated CO2 induces substantial and persistent declines in forage quality irrespective of warming in mixedgrass prairie.					
29297964	0	37	theme	Elevated	0:7	arg1	CO2					9:11	Elevated CO2	0:11	Elevated CO2	0:11	Elevated CO2 induces substantial and persistent declines in forage quality irrespective of warming in mixedgrass prairie.					
29297964	9	38	dep	small	1645:1649	arg1	e.g.					1652:1655	e.g.	1652:1655	e.g.	1652:1655	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	10	39	theme	declining	1953:1961	arg1	quality					1970:1976	declining forage quality	1953:1976	declining forage quality	1953:1976	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	2	40	theme	multiple	421:428	arg1	such					460:463	such	460:463	such	460:463	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	2	40	theme	multiple	421:428	arg1	pathways					450:457	multiple direct and indirect pathways	421:457	multiple direct and indirect pathways	421:457	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	4	41	dep	grasses	791:797	arg1	forage					784:789	forage	784:789	forage	784:789	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	5	42	theme	grass	1076:1080	arg1	[N					1052:1053	[N	1052:1053	[N	1052:1053	In contrast, eCO2 substantially reduced IVDMD (two most abundant grasses) and [N] (all three dominant grass species) in most years, except the two wettest years.					
29297964	5	42	theme	grass	1076:1080	arg1	species					1082:1088	dominant grass species	1067:1088	all three dominant grass species	1057:1088	In contrast, eCO2 substantially reduced IVDMD (two most abundant grasses) and [N] (all three dominant grass species) in most years, except the two wettest years.					
29297964	9	43	from	decline	1578:1584	arg1	IVDMD					1589:1593	IVDMD	1589:1593	IVDMD	1589:1593	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	9	44	theme	combined	1610:1617	arg1	warming					1619:1625	combined warming	1610:1625	combined warming	1610:1625	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	8	45	theme	total	1393:1397	arg1	production					1406:1415	total forage production	1393:1415	total forage production	1393:1415	For the six most abundant forage species (representing 96% of total forage production), combined warming and eCO2 increased forage production by 38% and reduced forage [N] by 13% relative to ambient climate.					
29297964	2	46	theme	elevated	350:357	arg1	eCO2					364:367	eCO2	364:367	eCO2	364:367	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	2	46	theme	elevated	350:357	arg1	CO2					359:361	elevated CO2	350:361	elevated CO2 (eCO2 )	350:369	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	8	47	theme	production	1406:1415	arg1	%					1388:1388	96%	1386:1388	96% of total forage production	1386:1415	For the six most abundant forage species (representing 96% of total forage production), combined warming and eCO2 increased forage production by 38% and reduced forage [N] by 13% relative to ambient climate.					
29297964	8	47	theme	production	1406:1415	arg1	production					1406:1415	total forage production	1393:1415	total forage production	1393:1415	For the six most abundant forage species (representing 96% of total forage production), combined warming and eCO2 increased forage production by 38% and reduced forage [N] by 13% relative to ambient climate.					
29297964	3	48	theme	+1.5°C	640:645	arg1	warming					631:637	infrared warming	622:637	infrared warming (+1.5°C day/3°C night)	622:660	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	3	48	theme	+1.5°C	640:645	arg1	night					655:659	+1.5°C day/3°C night	640:659	+1.5°C day/3°C night	640:659	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	3	49	theme	warming	631:637	arg1	effects					579:585	effects	579:585	effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night)	579:660	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	7	50	theme	tissues	1322:1328	arg1	content					1305:1311	acid detergent fiber (ADF) content	1278:1311	acid detergent fiber (ADF) content of grass tissues	1278:1328	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	9	51	theme	growing	1841:1847	arg1	season					1849:1854	the primary growing season	1829:1854	the primary growing season in the largest remaining rangeland ecosystem in North America	1829:1916	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	4	52	dep	negative	925:932	arg1	to					934:935	to	934:935	to	934:935	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	4	52	dep	negative	925:932	arg1	positive					937:944	positive	937:944	positive	937:944	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	5	53	theme	most	1094:1097	arg1	years					1099:1103	most years	1094:1103	most years	1094:1103	In contrast, eCO2 substantially reduced IVDMD (two most abundant grasses) and [N] (all three dominant grass species) in most years, except the two wettest years.					
29297964	4	54	theme	dry	828:830	arg1	IVDMD					854:858	IVDMD	854:858	IVDMD	854:858	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	4	54	theme	dry	828:830	arg1	digestibility					839:851	in vitro dry matter digestibility	819:851	in vitro dry matter digestibility (IVDMD)	819:859	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	7	55	theme	fiber	1293:1297	arg1	content					1305:1311	acid detergent fiber (ADF) content	1278:1311	acid detergent fiber (ADF) content of grass tissues	1278:1328	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	9	56	from	ecosystem	1891:1899	arg1	America					1910:1916	America	1910:1916	America	1910:1916	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	9	57	from	IVDMD	1589:1593	arg1	magnitude					1561:1569	the absolute magnitude	1548:1569	the absolute magnitude of the decline in IVDMD	1548:1593	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	10	58	theme	stocking	2034:2041	arg1	rates					2043:2047	stocking rates	2034:2047	stocking rates	2034:2047	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	7	59	theme	acid	1278:1281	arg1	content					1305:1311	acid detergent fiber (ADF) content	1278:1311	acid detergent fiber (ADF) content of grass tissues	1278:1328	Reduced IVDMD with eCO2 was related both to reduced [N] and increased acid detergent fiber (ADF) content of grass tissues.					
29297964	3	60	theme	field	678:682	arg1	conditions					684:693	realistic field conditions	668:693	realistic field conditions	668:693	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	4	61	theme	warming	800:806	arg1	effects					808:814	warming effects	800:814	warming effects on in vitro dry matter digestibility (IVDMD)	800:859	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	9	62	theme	rangeland	1881:1889	arg1	ecosystem					1891:1899	rangeland ecosystem	1881:1899	rangeland ecosystem in North America	1881:1916	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	6	63	theme	warming	1191:1197	arg1	effects					1199:1205	warming effects	1191:1205	warming effects	1191:1205	Furthermore, eCO2 reduced IVDMD and [N] independent of warming effects.					
29297964	8	64	dep	production	1406:1415	arg1	forage					1399:1404	forage	1399:1404	forage	1399:1404	For the six most abundant forage species (representing 96% of total forage production), combined warming and eCO2 increased forage production by 38% and reduced forage [N] by 13% relative to ambient climate.					
29297964	1	65	theme	species	209:215	arg1	composition					217:227	species composition	209:227	species composition	209:227	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	3	66	theme	7-yr	558:561	arg1	study					563:567	a 7-yr study	556:567	a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions	556:693	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	10	67	theme	prescribed	2081:2090	arg1	burning					2092:2098	prescribed burning	2081:2098	prescribed burning	2081:2098	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	1	68	dep	quantity	265:272	arg1	the					261:263	the	261:263	the	261:263	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	3	69	theme	enrichment	594:603	arg1	effects					579:585	effects	579:585	effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night)	579:660	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	1	70	theme	atmospheric	133:143	arg1	[CO2					145:148	Increasing atmospheric [CO2 ]	122:150	Increasing atmospheric [CO2 ]	122:150	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	10	71	dep	quality	1970:1976	arg1	forage					1963:1968	forage	1963:1968	forage	1963:1968	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	4	72	theme	certain	898:904	arg1	years					906:910	certain years	898:910	certain years	898:910	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	5	73	theme	wettest	1121:1127	arg1	years					1129:1133	the two wettest years	1113:1133	the two wettest years	1113:1133	In contrast, eCO2 substantially reduced IVDMD (two most abundant grasses) and [N] (all three dominant grass species) in most years, except the two wettest years.					
29297964	3	74	dep	quality	705:711	arg1	forage					698:703	forage	698:703	forage	698:703	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	2	75	dep	such	460:463	arg1	difficult					515:523	difficult	515:523	difficult	515:523	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	2	75	dep	such	460:463	arg1	outcomes					485:492	the cumulative outcomes	470:492	the cumulative outcomes of these effects	470:509	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	2	76	theme	cumulative	474:483	arg1	difficult					515:523	difficult	515:523	difficult	515:523	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	2	76	theme	cumulative	474:483	arg1	outcomes					485:492	the cumulative outcomes	470:492	the cumulative outcomes of these effects	470:509	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	10	77	theme	production	1931:1940	arg1	increases					1942:1950	forage production increases	1924:1950	forage production increases	1924:1950	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	10	78	dep	increases	1942:1950	arg1	forage					1924:1929	forage	1924:1929	forage	1924:1929	With forage production increases, declining forage quality could potentially be mitigated by adaptively increasing stocking rates, and through management such as prescribed burning, fertilization at low rates, and legume interseeding to enhance forage quality.					
29297964	9	79	theme	such	1730:1733	arg1	shifts					1735:1740	such shifts	1730:1740	such shifts	1730:1740	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	2	80	theme	effects	503:509	arg1	difficult					515:523	difficult	515:523	difficult	515:523	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	2	80	theme	effects	503:509	arg1	outcomes					485:492	the cumulative outcomes	470:492	the cumulative outcomes of these effects	470:509	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	3	81	from	quality	705:711	arg1	semiarid					731:738	a semiarid	729:738	a semiarid	729:738	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	3	81	from	quality	705:711	arg1	prairie					752:758	mixedgrass prairie	741:758	mixedgrass prairie	741:758	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	3	82	dep	600 ppm	609:615	arg1	to					606:607	to	606:607	to	606:607	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	2	83	theme	indirect	441:448	arg1	such					460:463	such	460:463	such	460:463	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	2	83	theme	indirect	441:448	arg1	pathways					450:457	multiple direct and indirect pathways	421:457	multiple direct and indirect pathways	421:457	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	3	84	from	quantity	717:724	arg1	semiarid					731:738	a semiarid	729:738	a semiarid	729:738	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	3	84	from	quantity	717:724	arg1	prairie					752:758	mixedgrass prairie	741:758	mixedgrass prairie	741:758	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	9	85	theme	substantial	1753:1763	arg1	consequences					1765:1776	substantial consequences	1753:1776	substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America	1753:1916	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	4	86	from	[N	872:873	arg1	IVDMD					854:858	IVDMD	854:858	IVDMD	854:858	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	4	86	from	[N	872:873	arg1	digestibility					839:851	in vitro dry matter digestibility	819:851	in vitro dry matter digestibility (IVDMD)	819:859	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	5	87	theme	dominant	1067:1074	arg1	[N					1052:1053	[N	1052:1053	[N	1052:1053	In contrast, eCO2 substantially reduced IVDMD (two most abundant grasses) and [N] (all three dominant grass species) in most years, except the two wettest years.					
29297964	5	87	theme	dominant	1067:1074	arg1	species					1082:1088	dominant grass species	1067:1088	all three dominant grass species	1057:1088	In contrast, eCO2 substantially reduced IVDMD (two most abundant grasses) and [N] (all three dominant grass species) in most years, except the two wettest years.					
29297964	2	88	theme	direct	430:435	arg1	such					460:463	such	460:463	such	460:463	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	2	88	theme	direct	430:435	arg1	pathways					450:457	multiple direct and indirect pathways	421:457	multiple direct and indirect pathways	421:457	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	1	89	theme	available	296:304	arg1	productivity					195:206	the productivity	191:206	the productivity	191:206	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	2	90	theme	tissue	396:401	arg1	chemistry					403:411	plant tissue chemistry	390:411	plant tissue chemistry	390:411	Both elevated CO2 (eCO2 ) and warming affect plant tissue chemistry through multiple direct and indirect pathways, such that the cumulative outcomes of these effects are difficult to predict.					
29297964	4	91	theme	dominant	775:782	arg1	grasses					791:797	the three dominant forage grasses	765:797	the three dominant forage grasses	765:797	For the three dominant forage grasses, warming effects on in vitro dry matter digestibility (IVDMD) and tissue [N] were detected only in certain years, varied from negative to positive, and were relatively minor.					
29297964	1	92	theme	rangeland	323:331	arg1	ecosystems					333:342	rangeland ecosystems	323:342	rangeland ecosystems	323:342	Increasing atmospheric [CO2 ] and temperature are expected to affect the productivity, species composition, biogeochemistry, and therefore the quantity and quality of forage available to herbivores in rangeland ecosystems.					
29297964	9	93	from	magnitude	1561:1569	arg1	IVDMD					1589:1593	IVDMD	1589:1593	IVDMD	1589:1593	Although the absolute magnitude of the decline in IVDMD and [N] due to combined warming and eCO2 may seem small (e.g., from 63.3 to 61.1% IVDMD and 1.25 to 1.04% [N] for Pascopyrum smithii), such shifts could have substantial consequences for the rate at which ruminants gain weight during the primary growing season in the largest remaining rangeland ecosystem in North America.					
29297964	8	94	theme	combined	1419:1426	arg1	warming					1428:1434	combined warming	1419:1434	combined warming	1419:1434	For the six most abundant forage species (representing 96% of total forage production), combined warming and eCO2 increased forage production by 38% and reduced forage [N] by 13% relative to ambient climate.					
29297964	3	95	theme	infrared	622:629	arg1	warming					631:637	infrared warming	622:637	infrared warming (+1.5°C day/3°C night)	622:660	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	3	95	theme	infrared	622:629	arg1	night					655:659	+1.5°C day/3°C night	640:659	+1.5°C day/3°C night	640:659	Here, we report on a 7-yr study examining effects of CO2 enrichment (to 600 ppm) and infrared warming (+1.5°C day/3°C night) under realistic field conditions on forage quality and quantity in a semiarid, mixedgrass prairie.					
29297964	8	96	dep	species	1364:1370	arg1	representing					1373:1384	representing	1373:1384	representing 96% of total forage production	1373:1415	For the six most abundant forage species (representing 96% of total forage production), combined warming and eCO2 increased forage production by 38% and reduced forage [N] by 13% relative to ambient climate.					
29297964	8	97	dep	abundant	1348:1355	arg1	forage					1357:1362	forage	1357:1362	forage species (representing 96% of total forage production)	1357:1416	For the six most abundant forage species (representing 96% of total forage production), combined warming and eCO2 increased forage production by 38% and reduced forage [N] by 13% relative to ambient climate.					
29806415	9	0	theme	groups	1754:1759	arg1	0°					1748:1749	0°	1748:1749	0° of groups B and C	1748:1767	Similarly, water static contact angle was from 76° of group A to 0° of groups B and C.					
29806415	12	1	theme	A	2105:2105	arg1	value					2108:2112	the absorbance ( A) value	2088:2112	the absorbance ( A) value	2088:2112	MTT test showed that hBMSCs proliferated well in 3 groups and the absorbance ( A) value was increased with time.					
29806415	0	2	theme	stem	234:237	arg1	cells					239:243	human bone marrow mesenchymal stem cells	204:243	human bone marrow mesenchymal stem cells	204:243	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	1	3	theme	protein	322:328	arg1	scaffolds					397:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	15	4	theme	mRNA	2444:2447	arg1	higher					2635:2640	higher	2635:2640	higher	2635:2640	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	4	theme	mRNA	2444:2447	arg1	expression					2458:2467	The mRNA relative expression	2440:2467	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days	2440:2523	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	6	5	theme	culture	1163:1169	arg1	conditions					1171:1180	chondrogenic culture conditions	1150:1180	chondrogenic culture conditions	1150:1180	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	6	6	dep	scaffolds	1136:1144	arg1	analyzed					1287:1294	analyzed	1287:1294	were analyzed	1282:1294	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	1	7	theme	human	526:530	arg1	marrow					537:542	human bone marrow mesenchymal stem cells (hBMSCs)	526:574	human bone marrow mesenchymal stem cells (hBMSCs)	526:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	15	8	theme	relative	2449:2456	arg1	higher					2635:2640	higher	2635:2640	higher	2635:2640	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	8	theme	relative	2449:2456	arg1	expression					2458:2467	The mRNA relative expression	2440:2467	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days	2440:2523	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	1	9	theme	marrow	537:542	arg1	differentiation					507:521	in vitro chondrogenic differentiation	485:521	in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs)	485:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	6	10	theme	cell	1247:1250	arg1	staining					1272:1279	Live-Dead staining	1262:1279	Live-Dead staining	1262:1279	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	6	10	theme	cell	1247:1250	arg1	activity					1252:1259	cell activity	1247:1259	cell activity (Live-Dead staining)	1247:1280	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	7	11	theme	0°/90°	1572:1577	arg1	orientation					1557:1567	fiber orientation	1551:1567	fiber orientation of 0°/90°	1551:1577	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	7	11	theme	0°/90°	1572:1577	arg1	porosity					1530:1537	porosity	1530:1537	porosity of 56%	1530:1544	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	7	11	theme	0°/90°	1572:1577	arg1	size					1510:1513	pore size	1505:1513	pore size of 400-500 μm	1505:1527	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	13	12	theme	group	2223:2227	arg1	B					2229:2229	group B	2223:2229	group B	2223:2229	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	15	13	theme	Ⅱafter	2486:2491	arg1	higher					2635:2640	higher	2635:2640	higher	2635:2640	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	13	theme	Ⅱafter	2486:2491	arg1	expression					2458:2467	The mRNA relative expression	2440:2467	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days	2440:2523	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	13	theme	Ⅱafter	2486:2491	arg1	expression					2548:2557	the mRNA relative expression	2530:2557	the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C	2530:2614	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	10	14	theme	cell	1803:1806	arg1	%					1857:1857	34.3%±3.5%	1848:1857	34.3%±3.5%	1848:1857	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	10	14	theme	cell	1803:1806	arg1	rate					1817:1820	the cell adhesion rate	1799:1820	the cell adhesion rate of groups A, B, and C	1799:1842	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	15	15	theme	type	2481:2484	arg1	Ⅱafter					2486:2491	collagen type Ⅱafter	2472:2491	collagen type Ⅱafter cultured for 7, 14, and 21 days	2472:2523	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	2	16	theme	porous	599:604	arg1	scaffold					613:620	A dimensional porous PCL-HA scaffold	585:620	A dimensional porous PCL-HA scaffold	585:620	Methods A dimensional porous PCL-HA scaffold was fabricated by 3D printing.					
29806415	15	17	theme	relative	2539:2546	arg1	higher					2635:2640	higher	2635:2640	higher	2635:2640	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	17	theme	relative	2539:2546	arg1	expression					2548:2557	the mRNA relative expression	2530:2557	the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C	2530:2614	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	5	18	theme	laden	1077:1081	arg1	scaffolds					1090:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	18	theme	laden	1077:1081	arg1	C					1045:1045	group C	1039:1045	group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	1039:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	8	19	theme	group	1664:1668	arg1	A					1670:1670	group A	1664:1670	group A	1664:1670	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	5	20	theme	cytological	892:902	arg1	experiment					904:913	The cytological experiment	888:913	The cytological experiment in vitro	888:922	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	2	21	theme	3D	640:641	arg1	printing					643:650	3D printing	640:650	3D printing	640:650	Methods A dimensional porous PCL-HA scaffold was fabricated by 3D printing.					
29806415	0	22	theme	mesenchymal	222:232	arg1	cells					239:243	human bone marrow mesenchymal stem cells	204:243	human bone marrow mesenchymal stem cells	204:243	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	10	23	theme	groups	1825:1830	arg1	%					1857:1857	34.3%±3.5%	1848:1857	34.3%±3.5%	1848:1857	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	10	23	theme	groups	1825:1830	arg1	rate					1817:1820	the cell adhesion rate	1799:1820	the cell adhesion rate of groups A, B, and C	1799:1842	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	0	24	theme	human	204:208	arg1	marrow					215:220	human bone marrow	204:220	human bone marrow mesenchymal stem cells	204:243	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	1	25	theme	3D	469:470	arg1	scaffolds					472:480	3D scaffolds	469:480	3D scaffolds	469:480	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	16	26	theme	dopamine	2776:2783	arg1	scaffolds					2817:2825	dopamine modified and CDMP1 laden PCL-HA scaffolds	2776:2825	dopamine modified and CDMP1 laden PCL-HA scaffolds	2776:2825	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.					
29806415	7	27	theme	%	1544:1544	arg1	orientation					1557:1567	fiber orientation	1551:1567	fiber orientation of 0°/90°	1551:1577	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	7	27	theme	%	1544:1544	arg1	porosity					1530:1537	porosity	1530:1537	porosity of 56%	1530:1544	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	7	27	theme	%	1544:1544	arg1	size					1510:1513	pore size	1505:1513	pore size of 400-500 μm	1505:1527	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	13	28	from	value	2145:2149	arg1	C					2160:2160	group C	2154:2160	group C	2154:2160	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	0	29	theme	marrow	215:220	arg1	cells					239:243	human bone marrow mesenchymal stem cells	204:243	human bone marrow mesenchymal stem cells	204:243	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	11	30	theme	good	1985:1988	arg1	activity					1995:2002	good cell activity	1985:2002	good cell activity of cells in 3 groups	1985:2023	Live/Dead staining showed good cell activity of cells in 3 groups.					
29806415	5	31	theme	dopamine	998:1005	arg1	scaffolds					1023:1031	dopamine modified PCL-HA scaffolds	998:1031	dopamine modified PCL-HA scaffolds	998:1031	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	31	theme	dopamine	998:1005	arg1	B					995:995	group B	989:995	group B (dopamine modified PCL-HA scaffolds)	989:1032	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	16	32	theme	laden	2804:2808	arg1	scaffolds					2817:2825	dopamine modified and CDMP1 laden PCL-HA scaffolds	2776:2825	dopamine modified and CDMP1 laden PCL-HA scaffolds	2776:2825	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.					
29806415	1	33	dep	marrow	537:542	arg1	hBMSCs					568:573	hBMSCs	568:573	hBMSCs	568:573	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	1	33	dep	marrow	537:542	arg1	cells					561:565	mesenchymal stem cells	544:565	human bone marrow mesenchymal stem cells (hBMSCs)	526:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	15	34	theme	group	2695:2699	arg1	all					2704:2706	all	2704:2706	all	2704:2706	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	34	theme	group	2695:2699	arg1	A					2701:2701	group A	2695:2701	group A	2695:2701	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	16	35	theme	PCL-HA	2810:2815	arg1	scaffolds					2817:2825	dopamine modified and CDMP1 laden PCL-HA scaffolds	2776:2825	dopamine modified and CDMP1 laden PCL-HA scaffolds	2776:2825	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.					
29806415	0	36	theme	composite	103:111	arg1	scaffolds					113:121	polycaprolactone-hydroxyapatite composite scaffolds	71:121	polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing	71:162	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	1	37	theme	three-dimensional	410:426	arg1	printing					433:440	three-dimensional (3D) printing	410:440	three-dimensional (3D) printing	410:440	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	11	38	from	activity	1995:2002	arg1	groups					2018:2023	3 groups	2016:2023	3 groups	2016:2023	Live/Dead staining showed good cell activity of cells in 3 groups.					
29806415	11	39	theme	cells	2007:2011	arg1	activity					1995:2002	good cell activity	1985:2002	good cell activity of cells in 3 groups	1985:2023	Live/Dead staining showed good cell activity of cells in 3 groups.					
29806415	5	40	theme	group	989:993	arg1	scaffolds					1023:1031	dopamine modified PCL-HA scaffolds	998:1031	dopamine modified PCL-HA scaffolds	998:1031	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	40	theme	group	989:993	arg1	B					995:995	group B	989:995	group B (dopamine modified PCL-HA scaffolds)	989:1032	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	40	theme	group	989:993	arg1	groups					953:958	3 groups	951:958	3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	951:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	15	41	theme	group	2679:2683	arg1	B					2685:2685	group B	2679:2685	group B	2679:2685	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	5	42	dep	groups	953:958	arg1	C					1045:1045	group C	1039:1045	group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	1039:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	42	dep	groups	953:958	arg1	scaffolds					1023:1031	dopamine modified PCL-HA scaffolds	998:1031	dopamine modified PCL-HA scaffolds	998:1031	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	42	dep	groups	953:958	arg1	A					967:967	group A	961:967	group A (PCL-HA scaffolds)	961:986	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	42	dep	groups	953:958	arg1	scaffolds					1090:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	42	dep	groups	953:958	arg1	B					995:995	group B	989:995	group B (dopamine modified PCL-HA scaffolds)	989:1032	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	42	dep	groups	953:958	arg1	scaffolds					977:985	PCL-HA scaffolds	970:985	PCL-HA scaffolds	970:985	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	42	dep	groups	953:958	arg1	groups					953:958	3 groups	951:958	3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	951:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	0	43	theme	three-dimensional	137:153	arg1	printing					155:162	three-dimensional printing	137:162	three-dimensional printing	137:162	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	15	44	dep	groups	2656:2661	arg1	groups					2656:2661	groups A and B	2656:2669	groups A and B	2656:2669	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	44	dep	groups	2656:2661	arg1	B					2669:2669	B	2669:2669	B	2669:2669	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	44	dep	groups	2656:2661	arg1	A					2663:2663	A	2663:2663	A	2663:2663	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	13	45	theme	group	2239:2243	arg1	A					2245:2245	group A	2239:2245	group A	2239:2245	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	9	46	theme	water	1694:1698	arg1	angle					1715:1719	water static contact angle	1694:1719	water static contact angle	1694:1719	Similarly, water static contact angle was from 76° of group A to 0° of groups B and C.					
29806415	4	47	theme	scanning	789:796	arg1	SEM					819:821	SEM	819:821	SEM	819:821	The surface microstructure was observed by scanning electron microscope (SEM) and porosity and water static contact angle were also detected.					
29806415	4	47	theme	scanning	789:796	arg1	microscope					807:816	scanning electron microscope	789:816	scanning electron microscope (SEM)	789:822	The surface microstructure was observed by scanning electron microscope (SEM) and porosity and water static contact angle were also detected.					
29806415	6	48	theme	quantitative	1393:1404	arg1	PCR					1406:1408	real-time fluorescent quantitative PCR	1371:1408	real-time fluorescent quantitative PCR	1371:1408	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	1	49	theme	in	485:486	arg1	differentiation					507:521	in vitro chondrogenic differentiation	485:521	in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs)	485:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	5	50	theme	PCL-HA	970:975	arg1	scaffolds					977:985	PCL-HA scaffolds	970:985	PCL-HA scaffolds	970:985	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	50	theme	PCL-HA	970:975	arg1	A					967:967	group A	961:967	group A (PCL-HA scaffolds)	961:986	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	14	51	theme	collagen	2368:2375	arg1	type					2377:2380	collagen type Ⅱ	2368:2382	collagen type Ⅱ	2368:2382	The mRNA relative expression of collagen type Ⅱ and Aggrecan increased gradually with time in 3 groups.					
29806415	9	52	theme	contact	1707:1713	arg1	angle					1715:1719	water static contact angle	1694:1719	water static contact angle	1694:1719	Similarly, water static contact angle was from 76° of group A to 0° of groups B and C.					
29806415	8	53	dep	groups	1624:1629	arg1	groups					1624:1629	groups B and C	1624:1637	groups B and C	1624:1637	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	8	53	dep	groups	1624:1629	arg1	C					1637:1637	C	1637:1637	C	1637:1637	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	8	53	dep	groups	1624:1629	arg1	B					1631:1631	B	1631:1631	B	1631:1631	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	6	54	theme	real-time	1371:1379	arg1	PCR					1406:1408	real-time fluorescent quantitative PCR	1371:1408	real-time fluorescent quantitative PCR	1371:1408	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	1	55	theme	laden	340:344	arg1	scaffolds					397:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	2	56	dep	Methods	577:583	arg1	fabricated					626:635	fabricated	626:635	was fabricated by 3D printing	622:650	Methods A dimensional porous PCL-HA scaffold was fabricated by 3D printing.					
29806415	14	57	theme	mRNA	2340:2343	arg1	expression					2354:2363	The mRNA relative expression	2336:2363	The mRNA relative expression of collagen type Ⅱ and Aggrecan	2336:2395	The mRNA relative expression of collagen type Ⅱ and Aggrecan increased gradually with time in 3 groups.					
29806415	6	58	theme	Aggrecan	1345:1352	arg1	expressions					1310:1320	the gene expressions	1301:1320	the gene expressions of collagen type Ⅱ and Aggrecan	1301:1352	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	16	59	theme	scaffolds	2817:2825	arg1	Co-culture					2762:2771	Conclusion Co-culture	2751:2771	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro	2751:2845	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.					
29806415	15	60	theme	significant	2716:2726	arg1	P<0.05					2742:2747	P<0.05	2742:2747	P<0.05	2742:2747	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	60	theme	significant	2716:2726	arg1	differences					2728:2738	significant differences	2716:2738	significant differences ( P<0.05)	2716:2748	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	5	61	theme	group	1039:1043	arg1	C					1045:1045	group C	1039:1045	group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	1039:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	61	theme	group	1039:1043	arg1	scaffolds					1090:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	61	theme	group	1039:1043	arg1	groups					953:958	3 groups	951:958	3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	951:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	9	62	theme	group	1737:1741	arg1	A					1743:1743	group A	1737:1743	group A	1737:1743	Similarly, water static contact angle was from 76° of group A to 0° of groups B and C.					
29806415	5	63	theme	modified	1007:1014	arg1	scaffolds					1023:1031	dopamine modified PCL-HA scaffolds	998:1031	dopamine modified PCL-HA scaffolds	998:1031	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	63	theme	modified	1007:1014	arg1	B					995:995	group B	989:995	group B (dopamine modified PCL-HA scaffolds)	989:1032	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	15	64	theme	Aggrecan	2562:2569	arg1	higher					2635:2640	higher	2635:2640	higher	2635:2640	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	64	theme	Aggrecan	2562:2569	arg1	expression					2458:2467	The mRNA relative expression	2440:2467	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days	2440:2523	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	64	theme	Aggrecan	2562:2569	arg1	expression					2548:2557	the mRNA relative expression	2530:2557	the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C	2530:2614	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	4	65	theme	static	847:852	arg1	angle					862:866	water static contact angle	841:866	water static contact angle	841:866	The surface microstructure was observed by scanning electron microscope (SEM) and porosity and water static contact angle were also detected.					
29806415	8	66	theme	dopamine	1584:1591	arg1	modification					1593:1604	dopamine modification	1584:1604	dopamine modification	1584:1604	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	1	67	theme	PCL-HA	379:384	arg1	scaffolds					397:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	0	68	theme	cells	239:243	arg1	differentiation					185:199	chondrogenic differentiation	172:199	chondrogenic differentiation of human bone marrow mesenchymal stem cells	172:243	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	1	69	theme	morphogenetic	308:320	arg1	protein					322:328	morphogenetic protein 1	308:330	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	1	69	theme	morphogenetic	308:320	arg1	CDMP1					333:337	CDMP1	333:337	CDMP1	333:337	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	15	70	from	days	2600:2603	arg1	C					2614:2614	group C	2608:2614	group C	2608:2614	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	1	71	dep	in	485:486	arg1	vitro					488:492	vitro	488:492	vitro	488:492	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	1	72	link	derived	300:306	arg1	scaffolds					397:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	1	73	theme	chondrogenic	494:505	arg1	differentiation					507:521	in vitro chondrogenic differentiation	485:521	in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs)	485:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	6	74	theme	chondrogenic	1150:1161	arg1	conditions					1171:1180	chondrogenic culture conditions	1150:1180	chondrogenic culture conditions	1150:1180	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	10	75	theme	%	1880:1880	arg1	%					1885:1885	57.4%±2.5%	1876:1885	57.4%±2.5% respectively	1876:1898	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	8	76	from	A	1670:1670	arg1	white					1676:1680	white	1676:1680	white	1676:1680	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	6	77	theme	collagen	1325:1332	arg1	type					1334:1337	collagen type Ⅱ	1325:1339	collagen type Ⅱ	1325:1339	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	6	78	theme	MTT	1231:1233	arg1	assay					1235:1239	MTT assay	1231:1239	MTT assay	1231:1239	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	6	78	theme	MTT	1231:1233	arg1	proliferation					1216:1228	the cell proliferation	1207:1228	the cell proliferation (MTT assay)	1207:1240	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	1	79	theme	bone	532:535	arg1	marrow					537:542	human bone marrow mesenchymal stem cells (hBMSCs)	526:574	human bone marrow mesenchymal stem cells (hBMSCs)	526:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	10	80	theme	significant	1909:1919	arg1	differences					1921:1931	significant differences	1909:1931	significant differences between groups ( P<0.05)	1909:1956	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	10	81	theme	adhesion	1808:1815	arg1	%					1857:1857	34.3%±3.5%	1848:1857	34.3%±3.5%	1848:1857	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	10	81	theme	adhesion	1808:1815	arg1	rate					1817:1820	the cell adhesion rate	1799:1820	the cell adhesion rate of groups A, B, and C	1799:1842	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	0	82	theme	chondrogenic	172:183	arg1	differentiation					185:199	chondrogenic differentiation	172:199	chondrogenic differentiation of human bone marrow mesenchymal stem cells	172:243	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	1	83	theme	mesenchymal	544:554	arg1	hBMSCs					568:573	hBMSCs	568:573	hBMSCs	568:573	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	1	83	theme	mesenchymal	544:554	arg1	cells					561:565	mesenchymal stem cells	544:565	human bone marrow mesenchymal stem cells (hBMSCs)	526:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	13	84	theme	significant	2301:2311	arg1	differences					2313:2323	significant differences	2301:2323	significant differences ( P<0.05)	2301:2333	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	13	84	theme	significant	2301:2311	arg1	P<0.05					2327:2332	P<0.05	2327:2332	P<0.05	2327:2332	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	7	85	from	scaffolds	1423:1431	arg1	groups					1438:1443	3 groups	1436:1443	3 groups	1436:1443	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	7	86	theme	fiber	1551:1555	arg1	orientation					1557:1567	fiber orientation	1551:1567	fiber orientation of 0°/90°	1551:1577	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	2	87	theme	PCL-HA	606:611	arg1	scaffold					613:620	A dimensional porous PCL-HA scaffold	585:620	A dimensional porous PCL-HA scaffold	585:620	Methods A dimensional porous PCL-HA scaffold was fabricated by 3D printing.					
29806415	5	88	theme	CDMP-1	1070:1075	arg1	scaffolds					1090:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	88	theme	CDMP-1	1070:1075	arg1	C					1045:1045	group C	1039:1045	group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	1039:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	10	89	theme	%	1864:1864	arg1	%					1869:1869	48.3%±1.5%	1860:1869	48.3%±1.5%	1860:1869	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	2	90	theme	dimensional	587:597	arg1	scaffold					613:620	A dimensional porous PCL-HA scaffold	585:620	A dimensional porous PCL-HA scaffold	585:620	Methods A dimensional porous PCL-HA scaffold was fabricated by 3D printing.					
29806415	12	91	theme	absorbance	2092:2101	arg1	value					2108:2112	the absorbance ( A) value	2088:2112	the absorbance ( A) value	2088:2112	MTT test showed that hBMSCs proliferated well in 3 groups and the absorbance ( A) value was increased with time.					
29806415	6	92	theme	cell	1211:1214	arg1	assay					1235:1239	MTT assay	1231:1239	MTT assay	1231:1239	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	6	92	theme	cell	1211:1214	arg1	proliferation					1216:1228	the cell proliferation	1207:1228	the cell proliferation (MTT assay)	1207:1240	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	8	93	from	white	1676:1680	arg1	A					1670:1670	group A	1664:1670	group A	1664:1670	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	0	94	theme	bone	210:213	arg1	marrow					215:220	human bone marrow	204:220	human bone marrow mesenchymal stem cells	204:243	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	5	95	theme	PCL-HA	1083:1088	arg1	scaffolds					1090:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	95	theme	PCL-HA	1083:1088	arg1	C					1045:1045	group C	1039:1045	group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	1039:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	16	96	theme	Conclusion	2751:2760	arg1	Co-culture					2762:2771	Conclusion Co-culture	2751:2771	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro	2751:2845	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.					
29806415	15	97	theme	collagen	2472:2479	arg1	Ⅱafter					2486:2491	collagen type Ⅱafter	2472:2491	collagen type Ⅱafter cultured for 7, 14, and 21 days	2472:2523	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	98	theme	mRNA	2534:2537	arg1	higher					2635:2640	higher	2635:2640	higher	2635:2640	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	15	98	theme	mRNA	2534:2537	arg1	expression					2548:2557	the mRNA relative expression	2530:2557	the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C	2530:2614	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	4	99	theme	surface	750:756	arg1	microstructure					758:771	The surface microstructure	746:771	The surface microstructure	746:771	The surface microstructure was observed by scanning electron microscope (SEM) and porosity and water static contact angle were also detected.					
29806415	6	100	theme	Live-Dead	1262:1270	arg1	staining					1272:1279	Live-Dead staining	1262:1279	Live-Dead staining	1262:1279	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	6	100	theme	Live-Dead	1262:1270	arg1	activity					1252:1259	cell activity	1247:1259	cell activity (Live-Dead staining)	1247:1280	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	1	101	theme	scaffolds	472:480	arg1	effect					459:464	the effect	455:464	the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs)	455:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	5	102	theme	group	961:965	arg1	scaffolds					977:985	PCL-HA scaffolds	970:985	PCL-HA scaffolds	970:985	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	102	theme	group	961:965	arg1	groups					953:958	3 groups	951:958	3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	951:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	102	theme	group	961:965	arg1	A					967:967	group A	961:967	group A (PCL-HA scaffolds)	961:986	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	16	103	theme	chondrogenic	2896:2907	arg1	differentiation					2909:2923	chondrogenic differentiation	2896:2923	chondrogenic differentiation	2896:2923	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.					
29806415	6	104	theme	adhesive	1192:1199	arg1	rate					1201:1204	the cell adhesive rate	1183:1204	the cell adhesive rate	1183:1204	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	0	105	theme	polycaprolactone-hydroxyapatite	71:101	arg1	scaffolds					113:121	polycaprolactone-hydroxyapatite composite scaffolds	71:121	polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing	71:162	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	5	106	theme	modified	1057:1064	arg1	scaffolds					1090:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	dopamine modified and CDMP-1 laden PCL-HA scaffolds	1048:1098	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	106	theme	modified	1057:1064	arg1	C					1045:1045	group C	1039:1045	group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds)	1039:1099	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	16	107	theme	modified	2785:2792	arg1	dopamine					2776:2783	dopamine modified	2776:2792	dopamine modified	2776:2792	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.					
29806415	10	108	theme	48.3	1860:1863	arg1	%					1869:1869	48.3%±1.5%	1860:1869	48.3%±1.5%	1860:1869	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	7	109	theme	μm	1526:1527	arg1	orientation					1557:1567	fiber orientation	1551:1567	fiber orientation of 0°/90°	1551:1577	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	7	109	theme	μm	1526:1527	arg1	porosity					1530:1537	porosity	1530:1537	porosity of 56%	1530:1544	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	7	109	theme	μm	1526:1527	arg1	size					1510:1513	pore size	1505:1513	pore size of 400-500 μm	1505:1527	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	11	110	theme	cell	1990:1993	arg1	activity					1995:2002	good cell activity	1985:2002	good cell activity of cells in 3 groups	1985:2023	Live/Dead staining showed good cell activity of cells in 3 groups.					
29806415	10	111	dep	groups	1825:1830	arg1	groups					1825:1830	groups A, B, and C	1825:1842	groups A, B, and C	1825:1842	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	10	111	dep	groups	1825:1830	arg1	B					1835:1835	B	1835:1835	B	1835:1835	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	10	111	dep	groups	1825:1830	arg1	C					1842:1842	C	1842:1842	C	1842:1842	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	10	111	dep	groups	1825:1830	arg1	A					1832:1832	A	1832:1832	A	1832:1832	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	14	112	theme	Aggrecan	2388:2395	arg1	expression					2354:2363	The mRNA relative expression	2336:2363	The mRNA relative expression of collagen type Ⅱ and Aggrecan	2336:2395	The mRNA relative expression of collagen type Ⅱ and Aggrecan increased gradually with time in 3 groups.					
29806415	3	113	used	used	666:669	arg2	Dopamine					653:660	Dopamine	653:660	Dopamine	653:660	Dopamine was used to modify the surface of PCL-HA and then CDMP-1 was loaded into scaffolds.					
29806415	6	114	theme	type	1334:1337	arg1	expressions					1310:1320	the gene expressions	1301:1320	the gene expressions of collagen type Ⅱ and Aggrecan	1301:1352	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	16	115	theme	CDMP1	2798:2802	arg1	laden					2804:2808	CDMP1 laden	2798:2808	CDMP1 laden	2798:2808	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.					
29806415	13	116	dep	groups	2200:2205	arg1	groups					2200:2205	groups B and A	2200:2213	groups B and A	2200:2213	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	13	116	dep	groups	2200:2205	arg1	A					2213:2213	A	2213:2213	A	2213:2213	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	13	116	dep	groups	2200:2205	arg1	B					2207:2207	B	2207:2207	B	2207:2207	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	16	117	theme	hBMSCs	2831:2836	arg1	Co-culture					2762:2771	Conclusion Co-culture	2751:2771	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro	2751:2845	Conclusion Co-culture of dopamine modified and CDMP1 laden PCL-HA scaffolds and hBMSCs in vitro can promote hBMSCs' adhesion, proliferation, and chondrogenic differentiation.					
29806415	10	118	theme	%	1852:1852	arg1	%					1857:1857	34.3%±3.5%	1848:1857	34.3%±3.5%	1848:1857	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	10	118	theme	%	1852:1852	arg1	rate					1817:1820	the cell adhesion rate	1799:1820	the cell adhesion rate of groups A, B, and C	1799:1842	After cultured for 24 hours, the cell adhesion rate of groups A, B, and C was 34.3%±3.5%, 48.3%±1.5%, and 57.4%±2.5% respectively, showing significant differences between groups ( P<0.05).					
29806415	1	119	from	effect	459:464	arg1	differentiation					507:521	in vitro chondrogenic differentiation	485:521	in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs)	485:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	7	120	theme	pore	1505:1508	arg1	size					1510:1513	pore size	1505:1513	pore size of 400-500 μm	1505:1527	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	13	121	theme	group	2154:2158	arg1	C					2160:2160	group C	2154:2160	group C	2154:2160	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	6	122	theme	gene	1305:1308	arg1	expressions					1310:1320	the gene expressions	1301:1320	the gene expressions of collagen type Ⅱ and Aggrecan	1301:1352	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	12	123	theme	MTT	2026:2028	arg1	test					2030:2033	MTT test	2026:2033	MTT test	2026:2033	MTT test showed that hBMSCs proliferated well in 3 groups and the absorbance ( A) value was increased with time.					
29806415	14	124	theme	type	2377:2380	arg1	expression					2354:2363	The mRNA relative expression	2336:2363	The mRNA relative expression of collagen type Ⅱ and Aggrecan	2336:2395	The mRNA relative expression of collagen type Ⅱ and Aggrecan increased gradually with time in 3 groups.					
29806415	9	125	theme	static	1700:1705	arg1	angle					1715:1719	water static contact angle	1694:1719	water static contact angle	1694:1719	Similarly, water static contact angle was from 76° of group A to 0° of groups B and C.					
29806415	1	126	dep	three-dimensional	410:426	arg1	3D					429:430	3D	429:430	3D	429:430	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	8	127	from	scaffolds	1611:1619	arg1	groups					1624:1629	groups B and C	1624:1637	groups B and C	1624:1637	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	8	127	from	scaffolds	1611:1619	arg1	C					1637:1637	C	1637:1637	C	1637:1637	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	8	127	from	scaffolds	1611:1619	arg1	B					1631:1631	B	1631:1631	B	1631:1631	For dopamine modification, the scaffolds in groups B and C were dark brown while in group A was white.					
29806415	6	128	theme	fluorescent	1381:1391	arg1	PCR					1406:1408	real-time fluorescent quantitative PCR	1371:1408	real-time fluorescent quantitative PCR	1371:1408	The hBMSCs were seeded into three scaffolds, in chondrogenic culture conditions, the cell adhesive rate, the cell proliferation (MTT assay), and cell activity (Live-Dead staining) were analyzed; and the gene expressions of collagen type Ⅱ and Aggrecan were detected by real-time fluorescent quantitative PCR.					
29806415	9	129	dep	groups	1754:1759	arg1	groups					1754:1759	groups B and C	1754:1767	groups B and C	1754:1767	Similarly, water static contact angle was from 76° of group A to 0° of groups B and C.					
29806415	9	129	dep	groups	1754:1759	arg1	C					1767:1767	C	1767:1767	C	1767:1767	Similarly, water static contact angle was from 76° of group A to 0° of groups B and C.					
29806415	9	129	dep	groups	1754:1759	arg1	B					1761:1761	B	1761:1761	B	1761:1761	Similarly, water static contact angle was from 76° of group A to 0° of groups B and C.					
29806415	13	130	theme	A	2143:2143	arg1	value					2145:2149	The A value	2139:2149	The A value in group C	2139:2160	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	13	130	theme	A	2143:2143	arg1	higher					2180:2185	higher	2180:2185	higher	2180:2185	The A value in group C was significantly higher than that in groups B and A, and in group B than in group A after cultured for 4, 7, 14, and 21 days, all showing significant differences ( P<0.05).					
29806415	3	131	theme	PCL-HA	696:701	arg1	surface					685:691	the surface	681:691	the surface of PCL-HA	681:701	Dopamine was used to modify the surface of PCL-HA and then CDMP-1 was loaded into scaffolds.					
29806415	4	132	theme	electron	798:805	arg1	SEM					819:821	SEM	819:821	SEM	819:821	The surface microstructure was observed by scanning electron microscope (SEM) and porosity and water static contact angle were also detected.					
29806415	4	132	theme	electron	798:805	arg1	microscope					807:816	scanning electron microscope	789:816	scanning electron microscope (SEM)	789:822	The surface microstructure was observed by scanning electron microscope (SEM) and porosity and water static contact angle were also detected.					
29806415	0	133	theme	morphogenetic	41:53	arg1	protein					55:61	morphogenetic protein 1	41:63	morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells	41:243	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	1	134	theme	polycaprolactone-hydroxyapatite	346:376	arg1	scaffolds					397:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	14	135	theme	relative	2345:2352	arg1	expression					2354:2363	The mRNA relative expression	2336:2363	The mRNA relative expression of collagen type Ⅱ and Aggrecan	2336:2395	The mRNA relative expression of collagen type Ⅱ and Aggrecan increased gradually with time in 3 groups.					
29806415	11	136	theme	Live/Dead	1959:1967	arg1	staining					1969:1976	Live/Dead staining	1959:1976	Live/Dead staining	1959:1976	Live/Dead staining showed good cell activity of cells in 3 groups.					
29806415	4	137	theme	contact	854:860	arg1	angle					862:866	water static contact angle	841:866	water static contact angle	841:866	The surface microstructure was observed by scanning electron microscope (SEM) and porosity and water static contact angle were also detected.					
29806415	1	138	theme	stem	556:559	arg1	hBMSCs					568:573	hBMSCs	568:573	hBMSCs	568:573	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	1	138	theme	stem	556:559	arg1	cells					561:565	mesenchymal stem cells	544:565	human bone marrow mesenchymal stem cells (hBMSCs)	526:574	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	7	139	dep	Results	1411:1417	arg1	showed					1454:1459	showed	1454:1459	were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°	1445:1577	Results The scaffolds in 3 groups were all showed a cross-linked and pore interconnected with pore size of 400-500 μm, porosity of 56%, and fiber orientation of 0°/90°.					
29806415	1	140	theme	composite	387:395	arg1	scaffolds					397:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	0	141	dep	cartilage	23:31	arg1	derived					33:39	derived	33:39	cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells	23:243	[Dopamine modified and cartilage derived morphogenetic protein 1 laden polycaprolactone-hydroxyapatite composite scaffolds fabricated by three-dimensional printing improve chondrogenic differentiation of human bone marrow mesenchymal stem cells].					
29806415	9	142	theme	A	1743:1743	arg1	76°					1730:1732	76°	1730:1732	76° of group A to 0° of groups B and C	1730:1767	Similarly, water static contact angle was from 76° of group A to 0° of groups B and C.					
29806415	1	143	dep	dopamine	268:275	arg1	cartilage					290:298	cartilage	290:298	cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing	290:440	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	1	143	dep	dopamine	268:275	arg1	modified					277:284	modified	277:284	modified	277:284	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
29806415	5	144	theme	PCL-HA	1016:1021	arg1	scaffolds					1023:1031	dopamine modified PCL-HA scaffolds	998:1031	dopamine modified PCL-HA scaffolds	998:1031	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	5	144	theme	PCL-HA	1016:1021	arg1	B					995:995	group B	989:995	group B (dopamine modified PCL-HA scaffolds)	989:1032	The cytological experiment in vitro were randomly divided into 3 groups: group A (PCL-HA scaffolds), group B (dopamine modified PCL-HA scaffolds), and group C (dopamine modified and CDMP-1 laden PCL-HA scaffolds).					
29806415	15	145	theme	group	2608:2612	arg1	C					2614:2614	group C	2608:2614	group C	2608:2614	The mRNA relative expression of collagen type Ⅱafter cultured for 7, 14, and 21 days, and the mRNA relative expression of Aggrecan after cultured for 14 and 21 days in group C were significantly higher than those in groups A and B, and in group B than in group A, all showing significant differences ( P<0.05).					
29806415	4	146	theme	water	841:845	arg1	angle					862:866	water static contact angle	841:866	water static contact angle	841:866	The surface microstructure was observed by scanning electron microscope (SEM) and porosity and water static contact angle were also detected.					
29806415	1	147	theme	derived	300:306	arg1	scaffolds					397:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds	300:405	Objective To prepare dopamine modified and cartilage derived morphogenetic protein 1 (CDMP1) laden polycaprolactone-hydroxyapatite (PCL-HA) composite scaffolds by three-dimensional (3D) printing and evaluate the effect of 3D scaffolds on in vitro chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs).					
30091673	0	0	from	degradation	33:43	arg1	brucei					108:113	Trypanosoma brucei	96:113	Trypanosoma brucei	96:113	Endoplasmic reticulum-associated degradation and disposal of misfolded GPI-anchored proteins in Trypanosoma brucei.					
30091673	6	1	theme	cytosol	856:862	arg1	fractions					864:872	cytosol fractions	856:872	cytosol fractions	856:872	HA:E6* partitions between membrane and cytosol fractions, and both pools are proteinase K-sensitive, indicating cytosolic disposition of membrane-associated HA:E6*.					
30091673	0	2	theme	Trypanosoma	96:106	arg1	brucei					108:113	Trypanosoma brucei	96:113	Trypanosoma brucei	96:113	Endoplasmic reticulum-associated degradation and disposal of misfolded GPI-anchored proteins in Trypanosoma brucei.					
30091673	8	3	theme	HA	1192:1193	arg1	*					1197:1197	HA:E6*	1192:1197	HA:E6*	1192:1197	However, HA:E6* is apparently not ubiquitin-modified.					
30091673	5	4	with	Treatment	675:683	arg1	inhibitor					710:718	a proteasome inhibitor	697:718	a proteasome inhibitor	697:718	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	5	4	with	Treatment	675:683	arg1	MG132					690:694	MG132	690:694	MG132	690:694	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	7	5	attach	removed	1086:1092	arg1	structure					1036:1044	a full GPI-glycan structure	1018:1044	a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation	1018:1180	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	7	5	attach	removed	1086:1092	arg2	dimyristoylglycerol					1057:1075	dimyristoylglycerol	1057:1075	dimyristoylglycerol	1057:1075	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	6	6	theme	*	979:979	arg1	disposition					939:949	cytosolic disposition	929:949	cytosolic disposition of membrane-associated HA:E6*	929:979	HA:E6* partitions between membrane and cytosol fractions, and both pools are proteinase K-sensitive, indicating cytosolic disposition of membrane-associated HA:E6*.					
30091673	5	7	theme	protected	741:749	arg1	HA					764:765	HA	764:765	HA:E6*	764:769	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	5	7	theme	protected	741:749	arg1	polypeptide					751:761	a smaller protected polypeptide	731:761	a smaller protected polypeptide (HA:E6*)	731:770	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	3	8	theme	trypanosome	559:569	arg1	receptor					583:590	the trypanosome transferrin receptor	555:590	the trypanosome transferrin receptor	555:590	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	6	9	theme	E6	977:978	arg1	*					979:979	membrane-associated HA:E6*	954:979	membrane-associated HA:E6*	954:979	HA:E6* partitions between membrane and cytosol fractions, and both pools are proteinase K-sensitive, indicating cytosolic disposition of membrane-associated HA:E6*.					
30091673	9	10	dep	ERQC	1330:1333	arg1	exit					1342:1345	exit	1342:1345	exit	1342:1345	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	3	11	theme	transferrin	571:581	arg1	receptor					583:590	the trypanosome transferrin receptor	555:590	the trypanosome transferrin receptor	555:590	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	3	12	theme	GPI-anchored	523:534	arg1	subunit					536:542	a misfolded GPI-anchored subunit	511:542	a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor	511:590	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	3	12	theme	GPI-anchored	523:534	arg1	HA					545:546	HA	545:546	HA:E6	545:549	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	4	13	dep	HA	593:594	arg1	E6					596:597	E6	596:597	HA:E6	593:597	HA:E6 is N-glycosylated and GPI-anchored and accumulates in the ER as aggregates.					
30091673	10	14	theme	model	1434:1438	arg1	systems					1440:1446	the standard eukaryotic model systems	1410:1446	the standard eukaryotic model systems	1410:1446	These results differ markedly from the standard eukaryotic model systems and may indicate an evolutionary advantage related to pathogenesis.					
30091673	3	15	theme	eukaryotic	466:475	arg1	brucei					498:503	the divergent eukaryotic pathogen Trypanosoma brucei	452:503	the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor	452:590	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	7	16	theme	complete	1111:1118	arg1	removal					1124:1130	complete GPI removal	1111:1130	complete GPI removal	1111:1130	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	7	16	theme	complete	1111:1118	arg1	prerequisite					1141:1152	a prerequisite	1139:1152	a prerequisite for proteasomal degradation	1139:1180	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	9	17	theme	trafficking	1277:1287	arg1	anchor					1257:1262	The trypanosome GPI anchor	1237:1262	The trypanosome GPI anchor	1237:1262	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	9	17	theme	trafficking	1277:1287	arg1	signal					1289:1294	a forward trafficking signal	1267:1294	a forward trafficking signal	1267:1294	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	10	18	theme	eukaryotic	1423:1432	arg1	systems					1440:1446	the standard eukaryotic model systems	1410:1446	the standard eukaryotic model systems	1410:1446	These results differ markedly from the standard eukaryotic model systems and may indicate an evolutionary advantage related to pathogenesis.					
30091673	5	19	with	consistent	773:782	arg1	turnover					789:796	turnover	789:796	turnover in the proteasome	789:814	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	7	20	contain	has	1014:1016	arg2	structure					1036:1044	a full GPI-glycan structure	1018:1044	a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation	1018:1180	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	7	20	contain	has	1014:1016	arg1	*					987:987	E6*	985:987	E6*	985:987	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	3	21	theme	pathogen	477:484	arg1	brucei					498:503	the divergent eukaryotic pathogen Trypanosoma brucei	452:503	the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor	452:590	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	0	22	theme	reticulum-associated	12:31	arg1	degradation					33:43	Endoplasmic reticulum-associated degradation	0:43	Endoplasmic reticulum-associated degradation	0:43	Endoplasmic reticulum-associated degradation and disposal of misfolded GPI-anchored proteins in Trypanosoma brucei.					
30091673	0	23	from	disposal	49:56	arg1	brucei					108:113	Trypanosoma brucei	96:113	Trypanosoma brucei	96:113	Endoplasmic reticulum-associated degradation and disposal of misfolded GPI-anchored proteins in Trypanosoma brucei.					
30091673	10	24	theme	standard	1414:1421	arg1	systems					1440:1446	the standard eukaryotic model systems	1410:1446	the standard eukaryotic model systems	1410:1446	These results differ markedly from the standard eukaryotic model systems and may indicate an evolutionary advantage related to pathogenesis.					
30091673	3	25	dep	HA	545:546	arg1	E6					548:549	E6	548:549	HA:E6	545:549	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	9	26	theme	forward	1269:1275	arg1	anchor					1257:1262	The trypanosome GPI anchor	1237:1262	The trypanosome GPI anchor	1237:1262	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	9	26	theme	forward	1269:1275	arg1	signal					1289:1294	a forward trafficking signal	1267:1294	a forward trafficking signal	1267:1294	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	0	27	theme	Endoplasmic	0:10	arg1	degradation					33:43	Endoplasmic reticulum-associated degradation	0:43	Endoplasmic reticulum-associated degradation	0:43	Endoplasmic reticulum-associated degradation and disposal of misfolded GPI-anchored proteins in Trypanosoma brucei.					
30091673	6	28	theme	HA	974:975	arg1	*					979:979	membrane-associated HA:E6*	954:979	membrane-associated HA:E6*	954:979	HA:E6* partitions between membrane and cytosol fractions, and both pools are proteinase K-sensitive, indicating cytosolic disposition of membrane-associated HA:E6*.					
30091673	4	29	gly	N-glycosylated	602:615	arg1	HA					593:594	HA	593:594	HA:E6	593:597	HA:E6 is N-glycosylated and GPI-anchored and accumulates in the ER as aggregates.					
30091673	1	30	theme	Misfolded	116:124	arg1	proteins					136:143	Misfolded secretory proteins	116:143	Misfolded secretory proteins	116:143	Misfolded secretory proteins are retained by endoplasmic reticulum quality control (ERQC) and degraded in the proteasome by ER-associated degradation (ERAD).					
30091673	3	31	theme	divergent	456:464	arg1	brucei					498:503	the divergent eukaryotic pathogen Trypanosoma brucei	452:503	the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor	452:590	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	6	32	theme	membrane-associated	954:972	arg1	*					979:979	membrane-associated HA:E6*	954:979	membrane-associated HA:E6*	954:979	HA:E6* partitions between membrane and cytosol fractions, and both pools are proteinase K-sensitive, indicating cytosolic disposition of membrane-associated HA:E6*.					
30091673	7	33	theme	GPI-glycan	1025:1034	arg1	structure					1036:1044	a full GPI-glycan structure	1018:1044	a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation	1018:1180	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	3	34	theme	receptor	583:590	arg1	subunit					536:542	a misfolded GPI-anchored subunit	511:542	a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor	511:590	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	3	34	theme	receptor	583:590	arg1	HA					545:546	HA	545:546	HA:E6	545:549	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	7	35	theme	GPI	1120:1122	arg1	removal					1124:1130	complete GPI removal	1111:1130	complete GPI removal	1111:1130	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	7	35	theme	GPI	1120:1122	arg1	prerequisite					1141:1152	a prerequisite	1139:1152	a prerequisite for proteasomal degradation	1139:1180	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	9	36	theme	dynamic	1306:1312	arg1	tension					1314:1320	the dynamic tension	1302:1320	the dynamic tension between ERQC and ER exit	1302:1345	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	9	37	theme	GPI	1253:1255	arg1	anchor					1257:1262	The trypanosome GPI anchor	1237:1262	The trypanosome GPI anchor	1237:1262	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	9	37	theme	GPI	1253:1255	arg1	signal					1289:1294	a forward trafficking signal	1267:1294	a forward trafficking signal	1267:1294	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	1	38	theme	secretory	126:134	arg1	proteins					136:143	Misfolded secretory proteins	116:143	Misfolded secretory proteins	116:143	Misfolded secretory proteins are retained by endoplasmic reticulum quality control (ERQC) and degraded in the proteasome by ER-associated degradation (ERAD).					
30091673	3	39	theme	Trypanosoma	486:496	arg1	brucei					498:503	the divergent eukaryotic pathogen Trypanosoma brucei	452:503	the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor	452:590	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	6	40	theme	cytosolic	929:937	arg1	disposition					939:949	cytosolic disposition	929:949	cytosolic disposition of membrane-associated HA:E6*	929:979	HA:E6* partitions between membrane and cytosol fractions, and both pools are proteinase K-sensitive, indicating cytosolic disposition of membrane-associated HA:E6*.					
30091673	5	41	theme	smaller	733:739	arg1	HA					764:765	HA	764:765	HA:E6*	764:769	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	5	41	theme	smaller	733:739	arg1	polypeptide					751:761	a smaller protected polypeptide	731:761	a smaller protected polypeptide (HA:E6*)	731:770	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	7	42	theme	E6	985:986	arg1	*					987:987	E6*	985:987	E6*	985:987	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	7	43	theme	proteasomal	1158:1168	arg1	degradation					1170:1180	proteasomal degradation	1158:1180	proteasomal degradation	1158:1180	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	0	44	theme	GPI-anchored	71:82	arg1	proteins					84:91	misfolded GPI-anchored proteins	61:91	misfolded GPI-anchored proteins	61:91	Endoplasmic reticulum-associated degradation and disposal of misfolded GPI-anchored proteins in Trypanosoma brucei.					
30091673	10	45	theme	related	1491:1497	arg1	advantage					1481:1489	an evolutionary advantage	1465:1489	an evolutionary advantage related to pathogenesis	1465:1513	These results differ markedly from the standard eukaryotic model systems and may indicate an evolutionary advantage related to pathogenesis.					
30091673	5	46	theme	E6	767:768	arg1	*					769:769	E6*	767:769	HA:E6*	764:769	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	10	47	theme	evolutionary	1468:1479	arg1	advantage					1481:1489	an evolutionary advantage	1465:1489	an evolutionary advantage related to pathogenesis	1465:1513	These results differ markedly from the standard eukaryotic model systems and may indicate an evolutionary advantage related to pathogenesis.					
30091673	7	48	theme	full	1020:1023	arg1	structure					1036:1044	a full GPI-glycan structure	1018:1044	a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation	1018:1180	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	0	49	theme	misfolded	61:69	arg1	proteins					84:91	misfolded GPI-anchored proteins	61:91	misfolded GPI-anchored proteins	61:91	Endoplasmic reticulum-associated degradation and disposal of misfolded GPI-anchored proteins in Trypanosoma brucei.					
30091673	5	50	dep	HA	764:765	arg1	*					769:769	E6*	767:769	HA:E6*	764:769	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	5	51	from	turnover	789:796	arg1	proteasome					805:814	the proteasome	801:814	the proteasome	801:814	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	2	52	theme	-anchored	349:357	arg1	proteins					359:366	misfolded glycosylphosphatidylinositol (GPI)-anchored proteins	305:366	misfolded glycosylphosphatidylinositol (GPI)-anchored proteins	305:366	However, in yeast and mammals, misfolded glycosylphosphatidylinositol (GPI)-anchored proteins are preferentially degraded in the vacuole/lysosome.					
30091673	5	53	theme	proteasome	699:708	arg1	inhibitor					710:718	a proteasome inhibitor	697:718	a proteasome inhibitor	697:718	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	5	53	theme	proteasome	699:708	arg1	MG132					690:694	MG132	690:694	MG132	690:694	Treatment with MG132, a proteasome inhibitor, generates a smaller protected polypeptide (HA:E6*), consistent with turnover in the proteasome.					
30091673	6	54	theme	E6	820:821	arg1	*					822:822	E6*	820:822	E6*	820:822	HA:E6* partitions between membrane and cytosol fractions, and both pools are proteinase K-sensitive, indicating cytosolic disposition of membrane-associated HA:E6*.					
30091673	3	55	theme	misfolded	513:521	arg1	subunit					536:542	a misfolded GPI-anchored subunit	511:542	a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor	511:590	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	3	55	theme	misfolded	513:521	arg1	HA					545:546	HA	545:546	HA:E6	545:549	We investigate this process in the divergent eukaryotic pathogen Trypanosoma brucei using a misfolded GPI-anchored subunit (HA:E6) of the trypanosome transferrin receptor.					
30091673	2	56	theme	misfolded	305:313	arg1	proteins					359:366	misfolded glycosylphosphatidylinositol (GPI)-anchored proteins	305:366	misfolded glycosylphosphatidylinositol (GPI)-anchored proteins	305:366	However, in yeast and mammals, misfolded glycosylphosphatidylinositol (GPI)-anchored proteins are preferentially degraded in the vacuole/lysosome.					
30091673	1	57	theme	ER-associated	240:252	arg1	ERAD					267:270	ERAD	267:270	ERAD	267:270	Misfolded secretory proteins are retained by endoplasmic reticulum quality control (ERQC) and degraded in the proteasome by ER-associated degradation (ERAD).					
30091673	1	57	theme	ER-associated	240:252	arg1	degradation					254:264	ER-associated degradation	240:264	ER-associated degradation (ERAD)	240:271	Misfolded secretory proteins are retained by endoplasmic reticulum quality control (ERQC) and degraded in the proteasome by ER-associated degradation (ERAD).					
30091673	1	58	theme	endoplasmic	161:171	arg1	reticulum					173:181	endoplasmic reticulum	161:181	endoplasmic reticulum quality control (ERQC)	161:204	Misfolded secretory proteins are retained by endoplasmic reticulum quality control (ERQC) and degraded in the proteasome by ER-associated degradation (ERAD).					
30091673	7	59	gly	de-N-glycosylated	992:1008	arg1	*					987:987	E6*	985:987	E6*	985:987	HA:E6* is de-N-glycosylated and has a full GPI-glycan structure from which dimyristoylglycerol has been removed, indicating that complete GPI removal is not a prerequisite for proteasomal degradation.					
30091673	1	60	theme	reticulum	173:181	arg1	ERQC					200:203	ERQC	200:203	ERQC	200:203	Misfolded secretory proteins are retained by endoplasmic reticulum quality control (ERQC) and degraded in the proteasome by ER-associated degradation (ERAD).					
30091673	1	60	theme	reticulum	173:181	arg1	control					191:197	endoplasmic reticulum quality control	161:197	endoplasmic reticulum quality control (ERQC)	161:204	Misfolded secretory proteins are retained by endoplasmic reticulum quality control (ERQC) and degraded in the proteasome by ER-associated degradation (ERAD).					
30091673	8	61	theme	E6	1195:1196	arg1	*					1197:1197	HA:E6*	1192:1197	HA:E6*	1192:1197	However, HA:E6* is apparently not ubiquitin-modified.					
30091673	0	62	theme	proteins	84:91	arg1	degradation					33:43	Endoplasmic reticulum-associated degradation	0:43	Endoplasmic reticulum-associated degradation	0:43	Endoplasmic reticulum-associated degradation and disposal of misfolded GPI-anchored proteins in Trypanosoma brucei.					
30091673	0	62	theme	proteins	84:91	arg1	disposal					49:56	disposal	49:56	disposal	49:56	Endoplasmic reticulum-associated degradation and disposal of misfolded GPI-anchored proteins in Trypanosoma brucei.					
30091673	9	63	theme	trypanosome	1241:1251	arg1	anchor					1257:1262	The trypanosome GPI anchor	1237:1262	The trypanosome GPI anchor	1237:1262	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	9	63	theme	trypanosome	1241:1251	arg1	signal					1289:1294	a forward trafficking signal	1267:1294	a forward trafficking signal	1267:1294	The trypanosome GPI anchor is a forward trafficking signal; thus the dynamic tension between ERQC and ER exit favors degradation by ERAD.					
30091673	1	64	theme	quality	183:189	arg1	ERQC					200:203	ERQC	200:203	ERQC	200:203	Misfolded secretory proteins are retained by endoplasmic reticulum quality control (ERQC) and degraded in the proteasome by ER-associated degradation (ERAD).					
30091673	1	64	theme	quality	183:189	arg1	control					191:197	endoplasmic reticulum quality control	161:197	endoplasmic reticulum quality control (ERQC)	161:204	Misfolded secretory proteins are retained by endoplasmic reticulum quality control (ERQC) and degraded in the proteasome by ER-associated degradation (ERAD).					
31539460	9	0	theme	rice	1819:1822	arg1	germination					1798:1808	germination	1798:1808	germination of brown rice	1798:1822	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	5	1	theme	rice	1145:1148	arg1	cultivar					1150:1157	rice cultivar	1145:1157	rice cultivar	1145:1157	The amount and composition of saccharides were strongly influenced by rice cultivar, and germination and mashing conditions.					
31539460	7	2	theme	PRACTICAL	1359:1367	arg1	APPLICATION					1369:1379	PRACTICAL APPLICATION	1359:1379	PRACTICAL APPLICATION: Rice syrup is commercially produced by the addition of external bacterial enzymes to brown or milled rice.	1359:1487	PRACTICAL APPLICATION: Rice syrup is commercially produced by the addition of external bacterial enzymes to brown or milled rice.					
31539460	4	3	theme	°C	1061:1062	arg1	temperatures					1042:1053	mashing temperatures	1034:1053	mashing temperatures of 75 °C and 85 °C	1034:1072	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	8	4	from	rich	1524:1527	arg1	amylases					1546:1553	amylases	1546:1553	amylases	1546:1553	Germinated brown rice is naturally rich in nutrients and amylases, both are produced during the germination process.					
31539460	8	4	from	rich	1524:1527	arg1	nutrients					1532:1540	nutrients	1532:1540	nutrients	1532:1540	Germinated brown rice is naturally rich in nutrients and amylases, both are produced during the germination process.					
31539460	9	5	from	germination	1798:1808	arg1	nutrients					1783:1791	the nutrients	1779:1791	the nutrients from germination of brown rice	1779:1822	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	0	6	theme	Cultivars	143:151	arg1	Saccharification					112:127	Starch Saccharification	105:127	Starch Saccharification of Brown Rice Cultivars During Syrup Production	105:175	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	2	7	theme	short	578:582	arg1	grain					584:588	short grain	578:588	short grain	578:588	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	4	8	theme	mashing	1034:1040	arg1	temperatures					1042:1053	mashing temperatures	1034:1053	mashing temperatures of 75 °C and 85 °C	1034:1072	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	6	9	theme	structure	1268:1276	arg1	importance					1227:1236	the importance	1223:1236	the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice	1223:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	1	10	theme	amylolytic	212:221	arg1	enzymes					223:229	amylolytic enzymes	212:229	amylolytic enzymes that can produce rice syrup	212:257	The germination process activates amylolytic enzymes that can produce rice syrup through mashing, however the factors affecting enzyme activities and soluble saccharides have not been investigated.					
31539460	4	11	theme	predominant	893:903	arg1	maltose					876:882	maltose	876:882	maltose	876:882	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	4	11	theme	predominant	893:903	arg1	Glucose					864:870	Glucose	864:870	Glucose	864:870	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	4	11	theme	predominant	893:903	arg1	saccharides					905:915	the predominant saccharides	889:915	the predominant saccharides	889:915	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	0	12	theme	Brown	132:136	arg1	Cultivars					143:151	Brown Rice Cultivars	132:151	Brown Rice Cultivars	132:151	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	2	13	from	cultivars	528:536	arg1	α-glucosidase					468:480	α-glucosidase	468:480	α-glucosidase	468:480	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	13	from	cultivars	528:536	arg1	α-amylase					442:450	α-amylase	442:450	α-amylase	442:450	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	13	from	cultivars	528:536	arg1	saccharides					495:505	soluble saccharides	487:505	soluble saccharides	487:505	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	13	from	cultivars	528:536	arg1	β-amylase					453:461	β-amylase	453:461	β-amylase	453:461	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	6	14	theme	rice	1241:1244	arg1	components					1246:1255	rice components	1241:1255	rice components	1241:1255	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	7	15	dep	APPLICATION	1369:1379	arg1	produced					1409:1416	produced	1409:1416	is commercially produced by the addition of external bacterial enzymes to brown or milled rice	1393:1486	PRACTICAL APPLICATION: Rice syrup is commercially produced by the addition of external bacterial enzymes to brown or milled rice.					
31539460	9	16	theme	brown	1674:1678	arg1	rice					1680:1683	germinated brown rice	1663:1683	germinated brown rice	1663:1683	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	0	17	theme	Syrup	160:164	arg1	Production					166:175	Syrup Production	160:175	Syrup Production	160:175	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	6	18	theme	saccharides	1319:1329	arg1	composition					1296:1306	composition	1296:1306	composition	1296:1306	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	6	18	theme	saccharides	1319:1329	arg1	saccharides					1319:1329	soluble saccharides	1311:1329	soluble saccharides from germinated brown rice	1311:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	6	18	theme	saccharides	1319:1329	arg1	amount					1285:1290	amount	1285:1290	amount	1285:1290	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	2	19	theme	rice	523:526	arg1	cultivars					528:536	germinated rice cultivars	512:536	germinated rice cultivars	512:536	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	5	20	theme	mashing	1180:1186	arg1	conditions					1188:1197	germination and mashing conditions	1164:1197	germination and mashing conditions	1164:1197	The amount and composition of saccharides were strongly influenced by rice cultivar, and germination and mashing conditions.					
31539460	0	21	theme	Enzyme	75:80	arg1	Activity					82:89	Amylolytic Enzyme Activity	64:89	Amylolytic Enzyme Activity	64:89	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	9	22	used	used	1694:1697	arg2	rice					1680:1683	germinated brown rice	1663:1683	germinated brown rice	1663:1683	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	0	23	from	Effect	0:5	arg1	Degree					95:100	Degree	95:100	Degree	95:100	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	0	23	from	Effect	0:5	arg1	Activity					82:89	Amylolytic Enzyme Activity	64:89	Amylolytic Enzyme Activity	64:89	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	3	24	theme	higher	774:779	arg1	activities					781:790	higher activities	774:790	higher activities of all three enzymes	774:811	The results showed that the long-grain rice had higher activities of all three enzymes, whereas the waxy rice exhibited lower activities.					
31539460	6	25	from	amount	1285:1290	arg1	rice					1353:1356	germinated brown rice	1336:1356	germinated brown rice	1336:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	4	26	dep	polymerization	990:1003	arg1	to					1007:1008	to	1007:1008	to	1007:1008	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	2	27	theme	rice	546:549	arg1	grain					614:618	long grain	609:618	long grain	609:618	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	27	theme	rice	546:549	arg1	cultivars					551:559	four rice cultivars	541:559	four rice cultivars	541:559	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	27	theme	rice	546:549	arg1	waxy					572:575	waxy	572:575	waxy	572:575	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	27	theme	rice	546:549	arg1	grain					584:588	short grain	578:588	short grain	578:588	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	27	theme	rice	546:549	arg1	grain					598:602	medium grain	591:602	medium grain	591:602	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	28	theme	anaerobic	639:647	arg1	conditions					661:670	aerobic and anaerobic germination conditions	627:670	aerobic and anaerobic germination conditions	627:670	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	6	29	from	composition	1296:1306	arg1	rice					1353:1356	germinated brown rice	1336:1356	germinated brown rice	1336:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	3	30	theme	lower	846:850	arg1	activities					852:861	lower activities	846:861	lower activities	846:861	The results showed that the long-grain rice had higher activities of all three enzymes, whereas the waxy rice exhibited lower activities.					
31539460	2	31	theme	enzymes	412:418	arg1	activities					420:429	amylolytic enzymes activities	401:429	amylolytic enzymes activities	401:429	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	31	theme	enzymes	412:418	arg1	α-amylase					442:450	α-amylase	442:450	α-amylase	442:450	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	31	theme	enzymes	412:418	arg1	α-glucosidase					468:480	α-glucosidase	468:480	α-glucosidase	468:480	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	31	theme	enzymes	412:418	arg1	saccharides					495:505	soluble saccharides	487:505	soluble saccharides	487:505	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	31	theme	enzymes	412:418	arg1	β-amylase					453:461	β-amylase	453:461	β-amylase	453:461	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	4	32	theme	degree	980:985	arg1	saccharides					965:975	saccharides	965:975	saccharides of degree of polymerization 3 to 7	965:1010	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	0	33	theme	Germination	10:20	arg1	Conditions					22:31	Germination Conditions	10:31	Germination Conditions	10:31	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	5	34	theme	saccharides	1105:1115	arg1	amount					1079:1084	amount	1079:1084	amount	1079:1084	The amount and composition of saccharides were strongly influenced by rice cultivar, and germination and mashing conditions.					
31539460	5	34	theme	saccharides	1105:1115	arg1	saccharides					1105:1115	saccharides	1105:1115	saccharides	1105:1115	The amount and composition of saccharides were strongly influenced by rice cultivar, and germination and mashing conditions.					
31539460	5	34	theme	saccharides	1105:1115	arg1	composition					1090:1100	composition	1090:1100	composition	1090:1100	The amount and composition of saccharides were strongly influenced by rice cultivar, and germination and mashing conditions.					
31539460	7	35	theme	milled	1476:1481	arg1	rice					1483:1486	milled rice	1476:1486	milled rice	1476:1486	PRACTICAL APPLICATION: Rice syrup is commercially produced by the addition of external bacterial enzymes to brown or milled rice.					
31539460	9	36	theme	enzymes	1754:1760	arg1	addition					1733:1740	the addition	1729:1740	the addition of external enzymes	1729:1760	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	8	37	theme	brown	1500:1504	arg1	rice					1506:1509	Germinated brown rice	1489:1509	Germinated brown rice	1489:1509	Germinated brown rice is naturally rich in nutrients and amylases, both are produced during the germination process.					
31539460	2	38	theme	soluble	487:493	arg1	saccharides					495:505	soluble saccharides	487:505	soluble saccharides	487:505	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	6	39	theme	germinated	1336:1345	arg1	rice					1353:1356	germinated brown rice	1336:1356	germinated brown rice	1336:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	1	40	theme	soluble	328:334	arg1	saccharides					336:346	soluble saccharides	328:346	soluble saccharides	328:346	The germination process activates amylolytic enzymes that can produce rice syrup through mashing, however the factors affecting enzyme activities and soluble saccharides have not been investigated.					
31539460	7	41	theme	enzymes	1456:1462	arg1	addition					1425:1432	the addition	1421:1432	the addition of external bacterial enzymes to brown or milled rice	1421:1486	PRACTICAL APPLICATION: Rice syrup is commercially produced by the addition of external bacterial enzymes to brown or milled rice.					
31539460	9	42	theme	germinated	1663:1672	arg1	rice					1680:1683	germinated brown rice	1663:1683	germinated brown rice	1663:1683	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	7	43	theme	external	1437:1444	arg1	enzymes					1456:1462	external bacterial enzymes	1437:1462	external bacterial enzymes	1437:1462	PRACTICAL APPLICATION: Rice syrup is commercially produced by the addition of external bacterial enzymes to brown or milled rice.					
31539460	3	44	contain	had	770:772	arg1	rice					765:768	the long-grain rice	750:768	the long-grain rice	750:768	The results showed that the long-grain rice had higher activities of all three enzymes, whereas the waxy rice exhibited lower activities.					
31539460	3	44	contain	had	770:772	arg2	activities					781:790	higher activities	774:790	higher activities of all three enzymes	774:811	The results showed that the long-grain rice had higher activities of all three enzymes, whereas the waxy rice exhibited lower activities.					
31539460	1	45	theme	rice	248:251	arg1	syrup					253:257	rice syrup	248:257	rice syrup	248:257	The germination process activates amylolytic enzymes that can produce rice syrup through mashing, however the factors affecting enzyme activities and soluble saccharides have not been investigated.					
31539460	4	46	theme	°C	961:962	arg1	temperatures					932:943	low mashing temperatures	920:943	low mashing temperatures of 55 °C and 65 °C	920:962	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	3	47	theme	long-grain	754:763	arg1	rice					765:768	the long-grain rice	750:768	the long-grain rice	750:768	The results showed that the long-grain rice had higher activities of all three enzymes, whereas the waxy rice exhibited lower activities.					
31539460	4	48	theme	polymerization	990:1003	arg1	degree					980:985	degree	980:985	degree of polymerization 3 to 7	980:1010	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	2	49	theme	long	609:612	arg1	grain					614:618	long grain	609:618	long grain	609:618	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	9	50	theme	activated	1643:1651	arg1	amylases					1653:1660	naturally activated amylases	1633:1660	naturally activated amylases	1633:1660	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	7	51	theme	Rice	1382:1385	arg1	syrup					1387:1391	Rice syrup	1382:1391	Rice syrup	1382:1391	PRACTICAL APPLICATION: Rice syrup is commercially produced by the addition of external bacterial enzymes to brown or milled rice.					
31539460	0	52	theme	Temperature	45:55	arg1	Effect					0:5	Effect	0:5	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.	0:176	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	2	53	theme	aerobic	627:633	arg1	conditions					661:670	aerobic and anaerobic germination conditions	627:670	aerobic and anaerobic germination conditions	627:670	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	4	54	theme	°C	1071:1072	arg1	temperatures					1042:1053	mashing temperatures	1034:1053	mashing temperatures of 75 °C and 85 °C	1034:1072	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	1	55	theme	germination	182:192	arg1	process					194:200	The germination process	178:200	The germination process	178:200	The germination process activates amylolytic enzymes that can produce rice syrup through mashing, however the factors affecting enzyme activities and soluble saccharides have not been investigated.					
31539460	8	56	from	amylases	1546:1553	arg1	rich					1524:1527	rich	1524:1527	rich	1524:1527	Germinated brown rice is naturally rich in nutrients and amylases, both are produced during the germination process.					
31539460	6	57	theme	starch	1261:1266	arg1	structure					1268:1276	starch structure	1261:1276	starch structure	1261:1276	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	9	58	theme	amylases	1653:1660	arg1	presence					1621:1628	the presence	1617:1628	the presence of naturally activated amylases	1617:1660	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	6	59	theme	components	1246:1255	arg1	importance					1227:1236	the importance	1223:1236	the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice	1223:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	2	60	theme	medium	591:596	arg1	grain					598:602	medium grain	591:602	medium grain	591:602	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	0	61	theme	Amylolytic	64:73	arg1	Activity					82:89	Amylolytic Enzyme Activity	64:89	Amylolytic Enzyme Activity	64:89	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	6	62	from	rice	1353:1356	arg1	composition					1296:1306	composition	1296:1306	composition	1296:1306	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	6	62	from	rice	1353:1356	arg1	saccharides					1319:1329	soluble saccharides	1311:1329	soluble saccharides from germinated brown rice	1311:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	6	62	from	rice	1353:1356	arg1	amount					1285:1290	amount	1285:1290	amount	1285:1290	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	3	63	theme	enzymes	805:811	arg1	activities					781:790	higher activities	774:790	higher activities of all three enzymes	774:811	The results showed that the long-grain rice had higher activities of all three enzymes, whereas the waxy rice exhibited lower activities.					
31539460	4	64	theme	mashing	924:930	arg1	temperatures					932:943	low mashing temperatures	920:943	low mashing temperatures of 55 °C and 65 °C	920:962	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	2	65	theme	cultivars	551:559	arg1	α-glucosidase					468:480	α-glucosidase	468:480	α-glucosidase	468:480	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	65	theme	cultivars	551:559	arg1	α-amylase					442:450	α-amylase	442:450	α-amylase	442:450	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	65	theme	cultivars	551:559	arg1	saccharides					495:505	soluble saccharides	487:505	soluble saccharides	487:505	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	65	theme	cultivars	551:559	arg1	β-amylase					453:461	β-amylase	453:461	β-amylase	453:461	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	6	66	theme	soluble	1311:1317	arg1	saccharides					1319:1329	soluble saccharides	1311:1329	soluble saccharides from germinated brown rice	1311:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	6	67	dep	amount	1285:1290	arg1	the					1281:1283	the	1281:1283	the	1281:1283	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	2	68	theme	germinated	512:521	arg1	cultivars					528:536	germinated rice cultivars	512:536	germinated rice cultivars	512:536	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	9	69	theme	rice	1710:1713	arg1	syrup					1715:1719	rice syrup	1710:1719	rice syrup	1710:1719	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	5	70	dep	amount	1079:1084	arg1	The					1075:1077	The	1075:1077	The	1075:1077	The amount and composition of saccharides were strongly influenced by rice cultivar, and germination and mashing conditions.					
31539460	2	71	theme	germination	649:659	arg1	conditions					661:670	aerobic and anaerobic germination conditions	627:670	aerobic and anaerobic germination conditions	627:670	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	0	72	theme	Starch	105:110	arg1	Saccharification					112:127	Starch Saccharification	105:127	Starch Saccharification of Brown Rice Cultivars During Syrup Production	105:175	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	4	73	theme	low	920:922	arg1	temperatures					932:943	low mashing temperatures	920:943	low mashing temperatures of 55 °C and 65 °C	920:962	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	0	74	dep	Activity	82:89	arg1	the					60:62	the	60:62	the	60:62	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	0	75	theme	Rice	138:141	arg1	Cultivars					143:151	Brown Rice Cultivars	132:151	Brown Rice Cultivars	132:151	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	8	76	theme	Germinated	1489:1498	arg1	rice					1506:1509	Germinated brown rice	1489:1509	Germinated brown rice	1489:1509	Germinated brown rice is naturally rich in nutrients and amylases, both are produced during the germination process.					
31539460	2	77	theme	amylolytic	401:410	arg1	activities					420:429	amylolytic enzymes activities	401:429	amylolytic enzymes activities	401:429	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	77	theme	amylolytic	401:410	arg1	α-amylase					442:450	α-amylase	442:450	α-amylase	442:450	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	77	theme	amylolytic	401:410	arg1	α-glucosidase					468:480	α-glucosidase	468:480	α-glucosidase	468:480	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	77	theme	amylolytic	401:410	arg1	saccharides					495:505	soluble saccharides	487:505	soluble saccharides	487:505	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	2	77	theme	amylolytic	401:410	arg1	β-amylase					453:461	β-amylase	453:461	β-amylase	453:461	This study characterized amylolytic enzymes activities, including α-amylase, β-amylase, and α-glucosidase, and soluble saccharides from germinated rice cultivars of four rice cultivars, including waxy, short grain, medium grain, and long grain, under aerobic and anaerobic germination conditions over 4 days and then mashed at 55, 65, 75, and 85 °C.					
31539460	0	78	theme	Conditions	22:31	arg1	Effect					0:5	Effect	0:5	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.	0:176	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	9	79	theme	external	1745:1752	arg1	enzymes					1754:1760	external enzymes	1745:1760	external enzymes	1745:1760	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	0	80	theme	Mashing	37:43	arg1	Temperature					45:55	Mashing Temperature	37:55	Mashing Temperature	37:55	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	1	81	theme	enzyme	306:311	arg1	activities					313:322	enzyme activities	306:322	enzyme activities	306:322	The germination process activates amylolytic enzymes that can produce rice syrup through mashing, however the factors affecting enzyme activities and soluble saccharides have not been investigated.					
31539460	4	82	theme	°C	951:952	arg1	temperatures					932:943	low mashing temperatures	920:943	low mashing temperatures of 55 °C and 65 °C	920:962	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	4	83	from	temperatures	932:943	arg1	maltose					876:882	maltose	876:882	maltose	876:882	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	4	83	from	temperatures	932:943	arg1	Glucose					864:870	Glucose	864:870	Glucose	864:870	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	4	83	from	temperatures	932:943	arg1	saccharides					905:915	the predominant saccharides	889:915	the predominant saccharides	889:915	Glucose and maltose were the predominant saccharides at low mashing temperatures of 55 °C and 65 °C; saccharides of degree of polymerization 3 to 7 became significant at mashing temperatures of 75 °C and 85 °C.					
31539460	6	84	theme	brown	1347:1351	arg1	rice					1353:1356	germinated brown rice	1336:1356	germinated brown rice	1336:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	6	85	from	importance	1227:1236	arg1	composition					1296:1306	composition	1296:1306	composition	1296:1306	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	6	85	from	importance	1227:1236	arg1	saccharides					1319:1329	soluble saccharides	1311:1329	soluble saccharides from germinated brown rice	1311:1356	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	6	85	from	importance	1227:1236	arg1	amount					1285:1290	amount	1285:1290	amount	1285:1290	The findings highlight the importance of rice components and starch structure on the amount and composition of soluble saccharides from germinated brown rice.					
31539460	8	86	from	nutrients	1532:1540	arg1	rich					1524:1527	rich	1524:1527	rich	1524:1527	Germinated brown rice is naturally rich in nutrients and amylases, both are produced during the germination process.					
31539460	8	87	theme	germination	1585:1595	arg1	process					1597:1603	the germination process	1581:1603	the germination process	1581:1603	Germinated brown rice is naturally rich in nutrients and amylases, both are produced during the germination process.					
31539460	7	88	theme	bacterial	1446:1454	arg1	enzymes					1456:1462	external bacterial enzymes	1437:1462	external bacterial enzymes	1437:1462	PRACTICAL APPLICATION: Rice syrup is commercially produced by the addition of external bacterial enzymes to brown or milled rice.					
31539460	9	89	theme	brown	1813:1817	arg1	rice					1819:1822	brown rice	1813:1822	brown rice	1813:1822	Because of the presence of naturally activated amylases, germinated brown rice could be used to produce rice syrup without the addition of external enzymes while preserving the nutrients from germination of brown rice.					
31539460	0	90	theme	Saccharification	112:127	arg1	Degree					95:100	Degree	95:100	Degree	95:100	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	0	90	theme	Saccharification	112:127	arg1	Activity					82:89	Amylolytic Enzyme Activity	64:89	Amylolytic Enzyme Activity	64:89	Effect of Germination Conditions and Mashing Temperature on the Amylolytic Enzyme Activity and Degree of Starch Saccharification of Brown Rice Cultivars During Syrup Production.					
31539460	5	91	theme	germination	1164:1174	arg1	conditions					1188:1197	germination and mashing conditions	1164:1197	germination and mashing conditions	1164:1197	The amount and composition of saccharides were strongly influenced by rice cultivar, and germination and mashing conditions.					
31054694	2	0	theme	mass	427:430	arg1	composition					381:391	composition	381:391	composition of the extraction solvent, sample mass and sonication time	381:450	Parameters that influence the extraction, such as composition of the extraction solvent, sample mass and sonication time were optimized in this work.					
31054694	1	1	from	Fe	282:283	arg1	sugar					315:319	non-centrifugal sugar	299:319	non-centrifugal sugar by F AAS	299:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	3	2	theme	non-centrifugal	608:622	arg1	sugar					624:628	non-centrifugal sugar	608:628	non-centrifugal sugar	608:628	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	6	3	theme	intermediate	908:919	arg1	precision					921:929	repeatability and intermediate precision	890:929	repeatability and intermediate precision	890:929	Estimates of repeatability and intermediate precision demonstrated that the precision observed was within the limits recommended in the literature.					
31054694	3	4	theme	sugar	624:628	arg1	H2O2					565:568	H2O2	565:568	H2O2 as an extraction solution	565:594	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	4	theme	sugar	624:628	arg1	60 min					634:639	60 min	634:639	60 min of sonication	634:653	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	4	theme	sugar	624:628	arg1	proportion					530:539	the proportion	526:539	the proportion of 60:40% (v/v) of HNO3	526:563	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	4	theme	sugar	624:628	arg1	0.100 g					597:603	0.100 g	597:603	0.100 g of non-centrifugal sugar	597:628	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	4	theme	sugar	624:628	arg1	condition					493:501	The optimum condition	481:501	The optimum condition for the extraction	481:520	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	1	5	theme	sample	233:238	arg1	treatment					240:248	a sample treatment	231:248	a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS	231:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	1	5	theme	sample	233:238	arg1	extraction					185:194	the ultrasound-assisted extraction	161:194	the ultrasound-assisted extraction	161:194	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	0	6	theme	micro	87:91	arg1	determination					70:82	the determination	66:82	the determination of micro and macro minerals in non-centrifugal sugar	66:135	Optimization and validation of ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar by F AAS.					
31054694	1	7	theme	Ca	293:294	arg1	determination					257:269	the determination	253:269	the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS	253:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	1	8	from	Zn	278:279	arg1	sugar					315:319	non-centrifugal sugar	299:319	non-centrifugal sugar by F AAS	299:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	0	9	theme	macro	97:101	arg1	determination					70:82	the determination	66:82	the determination of micro and macro minerals in non-centrifugal sugar	66:135	Optimization and validation of ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar by F AAS.					
31054694	1	10	theme	non-centrifugal	299:313	arg1	sugar					315:319	non-centrifugal sugar	299:319	non-centrifugal sugar by F AAS	299:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	7	11	theme	method	1046:1051	arg1	accuracy					1030:1037	Good accuracy	1025:1037	Good accuracy of the method	1025:1051	Good accuracy of the method was verified through the high recovery rates (91-108%).					
31054694	4	12	theme	Analytical	656:665	arg1	curves					667:672	Analytical curves	656:672	Analytical curves	656:672	Analytical curves were built in an aqueous solution.					
31054694	0	13	theme	non-centrifugal	115:129	arg1	sugar					131:135	non-centrifugal sugar	115:135	non-centrifugal sugar	115:135	Optimization and validation of ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar by F AAS.					
31054694	1	14	from	Mn	274:275	arg1	sugar					315:319	non-centrifugal sugar	299:319	non-centrifugal sugar by F AAS	299:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	6	15	theme	repeatability	890:902	arg1	precision					921:929	repeatability and intermediate precision	890:929	repeatability and intermediate precision	890:929	Estimates of repeatability and intermediate precision demonstrated that the precision observed was within the limits recommended in the literature.					
31054694	2	16	theme	time	447:450	arg1	composition					381:391	composition	381:391	composition of the extraction solvent, sample mass and sonication time	381:450	Parameters that influence the extraction, such as composition of the extraction solvent, sample mass and sonication time were optimized in this work.					
31054694	2	17	theme	sample	420:425	arg1	mass					427:430	sample mass	420:430	sample mass	420:430	Parameters that influence the extraction, such as composition of the extraction solvent, sample mass and sonication time were optimized in this work.					
31054694	1	18	from	Ca	293:294	arg1	sugar					315:319	non-centrifugal sugar	299:319	non-centrifugal sugar by F AAS	299:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	5	19	theme	Low	709:711	arg1	limits					728:733	Low quantification limits	709:733	Low quantification limits for Mn (0.021), Zn (0.008), Fe (0.030), Ca (0.389) and Mg (0.009) mg L-1	709:806	Low quantification limits for Mn (0.021), Zn (0.008), Fe (0.030), Ca (0.389) and Mg (0.009) mg L-1 were determined, suggesting a good detectability of the methodology.					
31054694	2	20	theme	solvent	411:417	arg1	composition					381:391	composition	381:391	composition of the extraction solvent, sample mass and sonication time	381:450	Parameters that influence the extraction, such as composition of the extraction solvent, sample mass and sonication time were optimized in this work.					
31054694	1	21	theme	F	324:324	arg1	AAS					326:328	F AAS	324:328	F AAS	324:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	1	22	from	determination	257:269	arg1	sugar					315:319	non-centrifugal sugar	299:319	non-centrifugal sugar by F AAS	299:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	1	23	theme	ultrasound-assisted	165:183	arg1	treatment					240:248	a sample treatment	231:248	a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS	231:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	1	23	theme	ultrasound-assisted	165:183	arg1	extraction					185:194	the ultrasound-assisted extraction	161:194	the ultrasound-assisted extraction	161:194	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	3	24	theme	extraction	576:585	arg1	solution					587:594	an extraction solution	573:594	an extraction solution	573:594	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	25	theme	sonication	644:653	arg1	H2O2					565:568	H2O2	565:568	H2O2 as an extraction solution	565:594	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	25	theme	sonication	644:653	arg1	60 min					634:639	60 min	634:639	60 min of sonication	634:653	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	25	theme	sonication	644:653	arg1	proportion					530:539	the proportion	526:539	the proportion of 60:40% (v/v) of HNO3	526:563	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	25	theme	sonication	644:653	arg1	0.100 g					597:603	0.100 g	597:603	0.100 g of non-centrifugal sugar	597:628	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	25	theme	sonication	644:653	arg1	condition					493:501	The optimum condition	481:501	The optimum condition for the extraction	481:520	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	0	26	dep	micro	87:91	arg1	minerals					103:110	minerals	103:110	minerals	103:110	Optimization and validation of ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar by F AAS.					
31054694	5	27	theme	quantification	713:726	arg1	limits					728:733	Low quantification limits	709:733	Low quantification limits for Mn (0.021), Zn (0.008), Fe (0.030), Ca (0.389) and Mg (0.009) mg L-1	709:806	Low quantification limits for Mn (0.021), Zn (0.008), Fe (0.030), Ca (0.389) and Mg (0.009) mg L-1 were determined, suggesting a good detectability of the methodology.					
31054694	3	28	theme	optimum	485:491	arg1	condition					493:501	The optimum condition	481:501	The optimum condition for the extraction	481:520	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	28	theme	optimum	485:491	arg1	proportion					530:539	the proportion	526:539	the proportion of 60:40% (v/v) of HNO3	526:563	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	1	29	from	sugar	315:319	arg1	determination					257:269	the determination	253:269	the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS	253:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	1	30	theme	Mn	274:275	arg1	determination					257:269	the determination	253:269	the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS	253:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	0	31	theme	extraction	51:60	arg1	validation					17:26	validation	17:26	validation	17:26	Optimization and validation of ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar by F AAS.					
31054694	0	31	theme	extraction	51:60	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization and validation of ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar by F AAS.					
31054694	3	32	theme	HNO3	560:563	arg1	v/v					552:554	v/v	552:554	v/v	552:554	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	32	theme	HNO3	560:563	arg1	HNO3					560:563	HNO3	560:563	HNO3	560:563	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	32	theme	HNO3	560:563	arg1	%					549:549	60:40%	544:549	60:40% (v/v) of HNO3	544:563	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	0	33	theme	ultrasound-assisted	31:49	arg1	extraction					51:60	ultrasound-assisted extraction	31:60	ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar	31:135	Optimization and validation of ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar by F AAS.					
31054694	1	34	theme	Zn	278:279	arg1	determination					257:269	the determination	253:269	the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS	253:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	0	35	theme	F	140:140	arg1	AAS					142:144	F AAS	140:144	F AAS	140:144	Optimization and validation of ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar by F AAS.					
31054694	7	36	theme	Good	1025:1028	arg1	accuracy					1030:1037	Good accuracy	1025:1037	Good accuracy of the method	1025:1051	Good accuracy of the method was verified through the high recovery rates (91-108%).					
31054694	1	37	from	Mg	286:287	arg1	sugar					315:319	non-centrifugal sugar	299:319	non-centrifugal sugar by F AAS	299:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	0	38	from	determination	70:82	arg1	sugar					131:135	non-centrifugal sugar	115:135	non-centrifugal sugar	115:135	Optimization and validation of ultrasound-assisted extraction for the determination of micro and macro minerals in non-centrifugal sugar by F AAS.					
31054694	1	39	from	treatment	240:248	arg1	determination					257:269	the determination	253:269	the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS	253:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	1	40	theme	Fe	282:283	arg1	determination					257:269	the determination	253:269	the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS	253:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	5	41	theme	good	838:841	arg1	detectability					843:855	a good detectability	836:855	a good detectability of the methodology	836:874	Low quantification limits for Mn (0.021), Zn (0.008), Fe (0.030), Ca (0.389) and Mg (0.009) mg L-1 were determined, suggesting a good detectability of the methodology.					
31054694	5	42	dep	Mn	739:740	arg1	 mg L-1					800:806	 mg L-1	800:806	 mg L-1	800:806	Low quantification limits for Mn (0.021), Zn (0.008), Fe (0.030), Ca (0.389) and Mg (0.009) mg L-1 were determined, suggesting a good detectability of the methodology.					
31054694	3	43	theme	%	549:549	arg1	H2O2					565:568	H2O2	565:568	H2O2 as an extraction solution	565:594	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	43	theme	%	549:549	arg1	60 min					634:639	60 min	634:639	60 min of sonication	634:653	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	43	theme	%	549:549	arg1	proportion					530:539	the proportion	526:539	the proportion of 60:40% (v/v) of HNO3	526:563	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	43	theme	%	549:549	arg1	0.100 g					597:603	0.100 g	597:603	0.100 g of non-centrifugal sugar	597:628	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	3	43	theme	%	549:549	arg1	condition					493:501	The optimum condition	481:501	The optimum condition for the extraction	481:520	The optimum condition for the extraction was the proportion of 60:40% (v/v) of HNO3:H2O2 as an extraction solution, 0.100 g of non-centrifugal sugar and 60 min of sonication.					
31054694	1	44	theme	first	217:221	arg1	time					223:226	the first time	213:226	the first time	213:226	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	7	45	theme	recovery	1083:1090	arg1	rates					1092:1096	the high recovery rates	1074:1096	the high recovery rates (91-108%)	1074:1106	Good accuracy of the method was verified through the high recovery rates (91-108%).					
31054694	7	45	theme	recovery	1083:1090	arg1	%					1105:1105	91-108%	1099:1105	91-108%	1099:1105	Good accuracy of the method was verified through the high recovery rates (91-108%).					
31054694	2	46	theme	sonication	436:445	arg1	time					447:450	sonication time	436:450	sonication time	436:450	Parameters that influence the extraction, such as composition of the extraction solvent, sample mass and sonication time were optimized in this work.					
31054694	5	47	theme	methodology	864:874	arg1	detectability					843:855	a good detectability	836:855	a good detectability of the methodology	836:874	Low quantification limits for Mn (0.021), Zn (0.008), Fe (0.030), Ca (0.389) and Mg (0.009) mg L-1 were determined, suggesting a good detectability of the methodology.					
31054694	7	48	theme	high	1078:1081	arg1	rates					1092:1096	the high recovery rates	1074:1096	the high recovery rates (91-108%)	1074:1106	Good accuracy of the method was verified through the high recovery rates (91-108%).					
31054694	7	48	theme	high	1078:1081	arg1	%					1105:1105	91-108%	1099:1105	91-108%	1099:1105	Good accuracy of the method was verified through the high recovery rates (91-108%).					
31054694	2	49	theme	extraction	400:409	arg1	solvent					411:417	the extraction solvent	396:417	the extraction solvent	396:417	Parameters that influence the extraction, such as composition of the extraction solvent, sample mass and sonication time were optimized in this work.					
31054694	1	50	theme	Mg	286:287	arg1	determination					257:269	the determination	253:269	the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS	253:328	In this work, the ultrasound-assisted extraction was employed for the first time as a sample treatment in the determination of Mn, Zn, Fe, Mg and Ca in non-centrifugal sugar by F AAS.					
31054694	4	51	theme	aqueous	691:697	arg1	solution					699:706	an aqueous solution	688:706	an aqueous solution	688:706	Analytical curves were built in an aqueous solution.					
31054694	6	52	theme	precision	921:929	arg1	Estimates					877:885	Estimates	877:885	Estimates of repeatability and intermediate precision	877:929	Estimates of repeatability and intermediate precision demonstrated that the precision observed was within the limits recommended in the literature.					
30879880	0	0	theme	Community	70:78	arg1	Composition					80:90	Vaginal Bacterial Community Composition	52:90	Vaginal Bacterial Community Composition in Black Adolescent Women	52:116	Vaginal Glycogen, Not Estradiol, Is Associated With Vaginal Bacterial Community Composition in Black Adolescent Women.					
30879880	1	1	theme	communities	314:324	arg1	composition					293:303	the species composition	281:303	the species composition of these communities	281:324	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	7	2	theme	Lactobacillus	1143:1155	arg1	proportions					1128:1138	low proportions	1124:1138	low proportions of Lactobacillus	1124:1155	Glycogen levels were higher in communities dominated by one or multiple species of Lactobacillus compared with those having low proportions of Lactobacillus.					
30879880	7	3	theme	Glycogen	1000:1007	arg1	levels					1009:1014	Glycogen levels	1000:1014	Glycogen levels	1000:1014	Glycogen levels were higher in communities dominated by one or multiple species of Lactobacillus compared with those having low proportions of Lactobacillus.					
30879880	5	4	theme	Lactobacillus	889:901	arg1	abundances					875:884	the abundances	871:884	the abundances of Lactobacillus	871:901	RESULTS Vaginal communities could be distinguished and classified into three groups that differed in the abundances of Lactobacillus.					
30879880	0	5	theme	Bacterial	60:68	arg1	Composition					80:90	Vaginal Bacterial Community Composition	52:90	Vaginal Bacterial Community Composition in Black Adolescent Women	52:116	Vaginal Glycogen, Not Estradiol, Is Associated With Vaginal Bacterial Community Composition in Black Adolescent Women.					
30879880	1	6	theme	study	147:151	arg1	purpose					131:137	The purpose	127:137	The purpose of this study	127:151	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	10	7	theme	simple	1597:1602	arg1	relationship					1604:1615	no simple relationship	1594:1615	no simple relationship between levels of estradiol and the production of vaginal glycogen	1594:1682	In addition, the results suggest there is no simple relationship between levels of estradiol and the production of vaginal glycogen.					
30879880	1	8	theme	black	237:241	arg1	women					254:258	black adolescent women	237:258	black adolescent women	237:258	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	6	9	contain	had	945:947	arg1	percent					915:921	Eighty-one percent	904:921	Eighty-one percent of study participants	904:943	Eighty-one percent of study participants had communities dominated by species of Lactobacillus.					
30879880	6	9	contain	had	945:947	arg2	communities					949:959	communities	949:959	communities dominated by species of Lactobacillus	949:997	Eighty-one percent of study participants had communities dominated by species of Lactobacillus.					
30879880	1	10	dep	PURPOSE	119:125	arg1	was					153:155	was	153:155	was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress	153:382	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	1	11	theme	adolescent	243:252	arg1	women					254:258	black adolescent women	237:258	black adolescent women	237:258	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	3	12	from	patterns	614:621	arg1	abundances					634:643	species abundances	626:643	species abundances	626:643	The composition of their vaginal communities was determined by analyzing the sequences of the V1-V3 regions of 16S rRNA genes, and they were grouped based on patterns in species abundances.					
30879880	1	13	theme	women	254:258	arg1	cohort					227:232	a cohort	225:232	a cohort of black adolescent women	225:258	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	0	14	theme	Adolescent	101:110	arg1	Women					112:116	Black Adolescent Women	95:116	Black Adolescent Women	95:116	Vaginal Glycogen, Not Estradiol, Is Associated With Vaginal Bacterial Community Composition in Black Adolescent Women.					
30879880	4	15	theme	psychosocial	693:704	arg1	stress					706:711	psychosocial stress	693:711	psychosocial stress	693:711	The relationships between estradiol, glycogen, psychosocial stress, and the composition of these communities were assessed.					
30879880	8	16	theme	positive	1292:1299	arg1	significant					1341:1351	significant	1341:1351	significant	1341:1351	Estradiol and psychosocial stress measurements did not differ among the three groups, whereas estradiol and glycogen exhibited a weak positive relationship that was not statistically significant.					
30879880	8	16	theme	positive	1292:1299	arg1	relationship					1301:1312	a weak positive relationship	1285:1312	a weak positive relationship that was not statistically significant	1285:1351	Estradiol and psychosocial stress measurements did not differ among the three groups, whereas estradiol and glycogen exhibited a weak positive relationship that was not statistically significant.					
30879880	9	17	theme	black	1487:1491	arg1	women					1493:1497	young black women	1481:1497	young black women	1481:1497	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30879880	0	18	theme	Black	95:99	arg1	Women					112:116	Black Adolescent Women	95:116	Black Adolescent Women	95:116	Vaginal Glycogen, Not Estradiol, Is Associated With Vaginal Bacterial Community Composition in Black Adolescent Women.					
30879880	1	19	theme	estradiol	352:360	arg1	levels					342:347	levels	342:347	levels of estradiol, glycogen, and stress	342:382	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	7	20	theme	low	1124:1126	arg1	proportions					1128:1138	low proportions	1124:1138	low proportions of Lactobacillus	1124:1155	Glycogen levels were higher in communities dominated by one or multiple species of Lactobacillus compared with those having low proportions of Lactobacillus.					
30879880	8	21	theme	psychosocial	1172:1183	arg1	stress					1185:1190	psychosocial stress	1172:1190	psychosocial stress	1172:1190	Estradiol and psychosocial stress measurements did not differ among the three groups, whereas estradiol and glycogen exhibited a weak positive relationship that was not statistically significant.					
30879880	9	22	theme	psychosocial	1523:1534	arg1	stress					1536:1541	psychosocial stress	1523:1541	psychosocial stress	1523:1541	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30879880	8	23	theme	weak	1287:1290	arg1	significant					1341:1351	significant	1341:1351	significant	1341:1351	Estradiol and psychosocial stress measurements did not differ among the three groups, whereas estradiol and glycogen exhibited a weak positive relationship that was not statistically significant.					
30879880	8	23	theme	weak	1287:1290	arg1	relationship					1301:1312	a weak positive relationship	1285:1312	a weak positive relationship that was not statistically significant	1285:1351	Estradiol and psychosocial stress measurements did not differ among the three groups, whereas estradiol and glycogen exhibited a weak positive relationship that was not statistically significant.					
30879880	0	24	theme	Vaginal	0:6	arg1	Glycogen					8:15	Vaginal Glycogen	0:15	Vaginal Glycogen	0:15	Vaginal Glycogen, Not Estradiol, Is Associated With Vaginal Bacterial Community Composition in Black Adolescent Women.					
30879880	6	25	theme	Lactobacillus	985:997	arg1	species					974:980	species	974:980	species of Lactobacillus	974:997	Eighty-one percent of study participants had communities dominated by species of Lactobacillus.					
30879880	8	26	dep	Estradiol	1158:1166	arg1	measurements					1192:1203	measurements	1192:1203	measurements	1192:1203	Estradiol and psychosocial stress measurements did not differ among the three groups, whereas estradiol and glycogen exhibited a weak positive relationship that was not statistically significant.					
30879880	3	27	theme	16S	567:569	arg1	genes					576:580	16S rRNA genes	567:580	16S rRNA genes	567:580	The composition of their vaginal communities was determined by analyzing the sequences of the V1-V3 regions of 16S rRNA genes, and they were grouped based on patterns in species abundances.					
30879880	10	28	theme	estradiol	1635:1643	arg1	production					1653:1662	the production	1649:1662	the production of vaginal glycogen	1649:1682	In addition, the results suggest there is no simple relationship between levels of estradiol and the production of vaginal glycogen.					
30879880	10	28	theme	estradiol	1635:1643	arg1	levels					1625:1630	levels	1625:1630	levels of estradiol	1625:1643	In addition, the results suggest there is no simple relationship between levels of estradiol and the production of vaginal glycogen.					
30879880	9	29	theme	pilot	1387:1391	arg1	study					1393:1397	this pilot study	1382:1397	this pilot study	1382:1397	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30879880	7	30	theme	Lactobacillus	1083:1095	arg1	Lactobacillus					1083:1095	Lactobacillus	1083:1095	Lactobacillus	1083:1095	Glycogen levels were higher in communities dominated by one or multiple species of Lactobacillus compared with those having low proportions of Lactobacillus.					
30879880	7	30	theme	Lactobacillus	1083:1095	arg1	one					1056:1058	one	1056:1058	one	1056:1058	Glycogen levels were higher in communities dominated by one or multiple species of Lactobacillus compared with those having low proportions of Lactobacillus.					
30879880	3	31	theme	V1-V3	550:554	arg1	regions					556:562	the V1-V3 regions	546:562	the V1-V3 regions of 16S rRNA genes	546:580	The composition of their vaginal communities was determined by analyzing the sequences of the V1-V3 regions of 16S rRNA genes, and they were grouped based on patterns in species abundances.					
30879880	2	32	theme	black	404:408	arg1	women					421:425	Twenty-one black adolescent women	393:425	Twenty-one black adolescent women	393:425	METHODS Twenty-one black adolescent women were sampled longitudinally.					
30879880	2	33	theme	adolescent	410:419	arg1	women					421:425	Twenty-one black adolescent women	393:425	Twenty-one black adolescent women	393:425	METHODS Twenty-one black adolescent women were sampled longitudinally.					
30879880	9	34	theme	study	1393:1397	arg1	findings					1370:1377	The findings	1366:1377	The findings of this pilot study	1366:1397	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30879880	1	35	theme	glycogen	363:370	arg1	levels					342:347	levels	342:347	levels of estradiol, glycogen, and stress	342:382	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	3	36	theme	regions	556:562	arg1	sequences					533:541	the sequences	529:541	the sequences of the V1-V3 regions of 16S rRNA genes	529:580	The composition of their vaginal communities was determined by analyzing the sequences of the V1-V3 regions of 16S rRNA genes, and they were grouped based on patterns in species abundances.					
30879880	2	37	theme	Twenty-one	393:402	arg1	women					421:425	Twenty-one black adolescent women	393:425	Twenty-one black adolescent women	393:425	METHODS Twenty-one black adolescent women were sampled longitudinally.					
30879880	10	38	theme	glycogen	1675:1682	arg1	production					1653:1662	the production	1649:1662	the production of vaginal glycogen	1649:1682	In addition, the results suggest there is no simple relationship between levels of estradiol and the production of vaginal glycogen.					
30879880	10	38	theme	glycogen	1675:1682	arg1	levels					1625:1630	levels	1625:1630	levels of estradiol	1625:1643	In addition, the results suggest there is no simple relationship between levels of estradiol and the production of vaginal glycogen.					
30879880	9	39	from	composition	1466:1476	arg1	women					1493:1497	young black women	1481:1497	young black women	1481:1497	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30879880	3	40	theme	species	626:632	arg1	abundances					634:643	species abundances	626:643	species abundances	626:643	The composition of their vaginal communities was determined by analyzing the sequences of the V1-V3 regions of 16S rRNA genes, and they were grouped based on patterns in species abundances.					
30879880	2	41	dep	METHODS	385:391	arg1	sampled					432:438	sampled	432:438	were sampled longitudinally	427:453	METHODS Twenty-one black adolescent women were sampled longitudinally.					
30879880	1	42	theme	vaginal	192:198	arg1	communities					210:220	vaginal bacterial communities	192:220	vaginal bacterial communities	192:220	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	5	43	theme	RESULTS	770:776	arg1	communities					786:796	RESULTS Vaginal communities	770:796	RESULTS Vaginal communities	770:796	RESULTS Vaginal communities could be distinguished and classified into three groups that differed in the abundances of Lactobacillus.					
30879880	10	44	theme	vaginal	1667:1673	arg1	glycogen					1675:1682	vaginal glycogen	1667:1682	vaginal glycogen	1667:1682	In addition, the results suggest there is no simple relationship between levels of estradiol and the production of vaginal glycogen.					
30879880	1	45	theme	bacterial	200:208	arg1	communities					210:220	vaginal bacterial communities	192:220	vaginal bacterial communities	192:220	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	5	46	theme	Vaginal	778:784	arg1	communities					786:796	RESULTS Vaginal communities	770:796	RESULTS Vaginal communities	770:796	RESULTS Vaginal communities could be distinguished and classified into three groups that differed in the abundances of Lactobacillus.					
30879880	1	47	theme	species	285:291	arg1	composition					293:303	the species composition	281:303	the species composition of these communities	281:324	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	1	48	theme	stress	377:382	arg1	levels					342:347	levels	342:347	levels of estradiol, glycogen, and stress	342:382	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	3	49	theme	rRNA	571:574	arg1	genes					576:580	16S rRNA genes	567:580	16S rRNA genes	567:580	The composition of their vaginal communities was determined by analyzing the sequences of the V1-V3 regions of 16S rRNA genes, and they were grouped based on patterns in species abundances.					
30879880	3	50	theme	vaginal	481:487	arg1	communities					489:499	their vaginal communities	475:499	their vaginal communities	475:499	The composition of their vaginal communities was determined by analyzing the sequences of the V1-V3 regions of 16S rRNA genes, and they were grouped based on patterns in species abundances.					
30879880	6	51	theme	Eighty-one	904:913	arg1	percent					915:921	Eighty-one percent	904:921	Eighty-one percent of study participants	904:943	Eighty-one percent of study participants had communities dominated by species of Lactobacillus.					
30879880	9	52	dep	CONCLUSIONS	1354:1364	arg1	suggest					1399:1405	suggest	1399:1405	suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.	1399:1550	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30879880	7	53	theme	multiple	1063:1070	arg1	species					1072:1078	multiple species	1063:1078	multiple species	1063:1078	Glycogen levels were higher in communities dominated by one or multiple species of Lactobacillus compared with those having low proportions of Lactobacillus.					
30879880	3	54	theme	genes	576:580	arg1	regions					556:562	the V1-V3 regions	546:562	the V1-V3 regions of 16S rRNA genes	546:580	The composition of their vaginal communities was determined by analyzing the sequences of the V1-V3 regions of 16S rRNA genes, and they were grouped based on patterns in species abundances.					
30879880	1	55	from	composition	177:187	arg1	cohort					227:232	a cohort	225:232	a cohort of black adolescent women	225:258	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	3	56	theme	communities	489:499	arg1	composition					460:470	The composition	456:470	The composition of their vaginal communities	456:499	The composition of their vaginal communities was determined by analyzing the sequences of the V1-V3 regions of 16S rRNA genes, and they were grouped based on patterns in species abundances.					
30879880	6	57	theme	participants	932:943	arg1	percent					915:921	Eighty-one percent	904:921	Eighty-one percent of study participants	904:943	Eighty-one percent of study participants had communities dominated by species of Lactobacillus.					
30879880	1	58	theme	communities	210:220	arg1	composition					177:187	the composition	173:187	the composition of vaginal bacterial communities in a cohort of black adolescent women	173:258	PURPOSE The purpose of this study was to characterize the composition of vaginal bacterial communities in a cohort of black adolescent women and to determine how the species composition of these communities correlates with levels of estradiol, glycogen, and stress.					
30879880	4	59	theme	communities	743:753	arg1	composition					722:732	the composition	718:732	the composition of these communities	718:753	The relationships between estradiol, glycogen, psychosocial stress, and the composition of these communities were assessed.					
30879880	4	59	theme	communities	743:753	arg1	glycogen					683:690	glycogen	683:690	glycogen	683:690	The relationships between estradiol, glycogen, psychosocial stress, and the composition of these communities were assessed.					
30879880	4	59	theme	communities	743:753	arg1	stress					706:711	psychosocial stress	693:711	psychosocial stress	693:711	The relationships between estradiol, glycogen, psychosocial stress, and the composition of these communities were assessed.					
30879880	4	59	theme	communities	743:753	arg1	estradiol					672:680	estradiol	672:680	estradiol	672:680	The relationships between estradiol, glycogen, psychosocial stress, and the composition of these communities were assessed.					
30879880	0	60	theme	Vaginal	52:58	arg1	Composition					80:90	Vaginal Bacterial Community Composition	52:90	Vaginal Bacterial Community Composition in Black Adolescent Women	52:116	Vaginal Glycogen, Not Estradiol, Is Associated With Vaginal Bacterial Community Composition in Black Adolescent Women.					
30879880	0	61	from	Composition	80:90	arg1	Women					112:116	Black Adolescent Women	95:116	Black Adolescent Women	95:116	Vaginal Glycogen, Not Estradiol, Is Associated With Vaginal Bacterial Community Composition in Black Adolescent Women.					
30879880	0	62	dep	Glycogen	8:15	arg1	Estradiol					22:30	Estradiol	22:30	Estradiol	22:30	Vaginal Glycogen, Not Estradiol, Is Associated With Vaginal Bacterial Community Composition in Black Adolescent Women.					
30879880	8	63	dep	differ	1213:1218	arg1	whereas					1244:1250	whereas	1244:1250	whereas	1244:1250	Estradiol and psychosocial stress measurements did not differ among the three groups, whereas estradiol and glycogen exhibited a weak positive relationship that was not statistically significant.					
30879880	7	64	contain	having	1117:1122	arg1	those					1111:1115	those	1111:1115	those	1111:1115	Glycogen levels were higher in communities dominated by one or multiple species of Lactobacillus compared with those having low proportions of Lactobacillus.					
30879880	7	64	contain	having	1117:1122	arg2	proportions					1128:1138	low proportions	1124:1138	low proportions of Lactobacillus	1124:1155	Glycogen levels were higher in communities dominated by one or multiple species of Lactobacillus compared with those having low proportions of Lactobacillus.					
30879880	9	65	theme	vaginal	1448:1454	arg1	composition					1466:1476	vaginal community composition	1448:1476	vaginal community composition in young black women	1448:1497	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30879880	9	66	theme	young	1481:1485	arg1	women					1493:1497	young black women	1481:1497	young black women	1481:1497	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30879880	6	67	theme	study	926:930	arg1	participants					932:943	study participants	926:943	study participants	926:943	Eighty-one percent of study participants had communities dominated by species of Lactobacillus.					
30879880	9	68	theme	community	1456:1464	arg1	composition					1466:1476	vaginal community composition	1448:1476	vaginal community composition in young black women	1448:1497	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30879880	9	69	theme	glycogen	1412:1419	arg1	levels					1421:1426	glycogen levels	1412:1426	glycogen levels	1412:1426	CONCLUSIONS The findings of this pilot study suggest that glycogen levels are associated with vaginal community composition in young black women; however, estradiol and psychosocial stress are not.					
30659765	7	0	theme	structural	1150:1159	arg1	analysis					1161:1168	structural analysis	1150:1168	structural analysis	1150:1168	Furthermore, structural analysis provides new insights regarding the lack of mannose monosaccharides, especially in the WSG synthesis of the SmudexA mutants.					
30659765	1	1	theme	cariogenic	337:346	arg1	pathogenicity					348:360	its cariogenic pathogenicity	333:360	its cariogenic pathogenicity	333:360	The cariogenic pathogen Streptococcus mutans effectively utilizes dietary sucrose for the synthesis of exopolysaccharides (EPS), which act as a scaffold for its biofilm and thus contribute to its cariogenic pathogenicity.					
30659765	3	2	theme	mutans	698:703	arg1	biofilms					705:712	S mutans biofilms	696:712	S mutans biofilms	696:712	Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutans biofilms.					
30659765	5	3	theme	dexA	865:868	arg1	deletion					853:860	deletion	853:860	deletion of dexA (SmudexA)	853:878	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	0	4	theme	gene	78:81	arg1	aggregation					99:109	biofilm aggregation	91:109	biofilm aggregation	91:109	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	0	4	theme	gene	78:81	arg1	pathogenicity					126:138	cariogenic pathogenicity	115:138	cariogenic pathogenicity	115:138	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	0	4	theme	gene	78:81	arg1	effects					83:89	gene effects biofilm aggregation and cariogenic pathogenicity	78:138	gene effects biofilm aggregation and cariogenic pathogenicity	78:138	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	8	5	theme	cariogenic	1468:1477	arg1	incidence					1479:1487	the cariogenic incidence	1464:1487	the cariogenic incidence of the SmudexA mutants	1464:1510	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	10	6	theme	dental	1821:1826	arg1	management					1835:1844	dental caries management	1821:1844	dental caries management	1821:1844	These results highlight dexA targeting as a potentially effective tool in dental caries management.					
30659765	4	7	from	role	746:749	arg1	aggregation					797:807	dextran-dependent aggregation	779:807	dextran-dependent aggregation in biofilm formation	779:828	In this study, we identify the role of the S mutans dexAgene in dextran-dependent aggregation in biofilm formation.					
30659765	8	8	theme	biofilm	1299:1305	arg1	phenotypes					1307:1316	The biofilm phenotypes	1295:1316	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift	1295:1433	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	10	9	theme	dexA	1771:1774	arg1	tool					1813:1816	a potentially effective tool	1789:1816	a potentially effective tool in dental caries management	1789:1844	These results highlight dexA targeting as a potentially effective tool in dental caries management.					
30659765	10	9	theme	dexA	1771:1774	arg1	targeting					1776:1784	dexA targeting	1771:1784	dexA targeting	1771:1784	These results highlight dexA targeting as a potentially effective tool in dental caries management.					
30659765	8	10	theme	monosaccharide	1363:1376	arg1	composition					1378:1388	the reduced glucose monosaccharide composition	1343:1388	the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift	1343:1433	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	2	11	theme	limited	558:564	arg1	attention					566:574	limited attention	558:574	limited attention	558:574	Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention.					
30659765	9	12	theme	synthesis	1556:1564	arg1	fine-tuning					1566:1576	EPS synthesis fine-tuning	1552:1576	EPS synthesis fine-tuning by the dexA gene	1552:1593	Taken together, these data reveal that EPS synthesis fine-tuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria.					
30659765	5	13	theme	synthesis-related	922:938	arg1	gtfD					963:966	gtfD	963:966	gtfD	963:966	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	5	13	theme	synthesis-related	922:938	arg1	genes					940:944	EPS synthesis-related genes	918:944	EPS synthesis-related genes	918:944	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	5	13	theme	synthesis-related	922:938	arg1	gtfB					957:960	gtfB	957:960	gtfB	957:960	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	5	13	theme	synthesis-related	922:938	arg1	ftf					973:975	ftf	973:975	ftf	973:975	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	2	14	theme	structural	487:496	arg1	features					498:505	the structural features	483:505	the structural features of the EPS regulated by the dexAgene	483:542	Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention.					
30659765	5	15	theme	genes	940:944	arg1	transcription					901:913	increased transcription	891:913	increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf	891:975	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	6	16	theme	WSG	1056:1058	arg1	synthesis					1060:1068	elevated WSG synthesis	1047:1068	elevated WSG synthesis	1047:1068	Interestingly, we reveal that inactivating the dexA gene may lead to elevated WSG synthesis in S mutans , which results in dysregulated cariogenicity in vivo.					
30659765	0	17	theme	cariogenic	115:124	arg1	pathogenicity					126:138	cariogenic pathogenicity	115:138	cariogenic pathogenicity	115:138	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	0	17	theme	cariogenic	115:124	arg1	effects					83:89	gene effects biofilm aggregation and cariogenic pathogenicity	78:138	gene effects biofilm aggregation and cariogenic pathogenicity	78:138	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	7	18	theme	monosaccharides	1222:1236	arg1	lack					1206:1209	the lack	1202:1209	the lack of mannose monosaccharides	1202:1236	Furthermore, structural analysis provides new insights regarding the lack of mannose monosaccharides, especially in the WSG synthesis of the SmudexA mutants.					
30659765	4	19	theme	biofilm	812:818	arg1	formation					820:828	biofilm formation	812:828	biofilm formation	812:828	In this study, we identify the role of the S mutans dexAgene in dextran-dependent aggregation in biofilm formation.					
30659765	7	20	theme	WSG	1257:1259	arg1	synthesis					1261:1269	the WSG synthesis	1253:1269	the WSG synthesis of the SmudexA mutants	1253:1292	Furthermore, structural analysis provides new insights regarding the lack of mannose monosaccharides, especially in the WSG synthesis of the SmudexA mutants.					
30659765	6	21	theme	dysregulated	1101:1112	arg1	cariogenicity					1114:1126	dysregulated cariogenicity	1101:1126	dysregulated cariogenicity	1101:1126	Interestingly, we reveal that inactivating the dexA gene may lead to elevated WSG synthesis in S mutans , which results in dysregulated cariogenicity in vivo.					
30659765	9	22	theme	energy	1715:1720	arg1	source					1722:1727	an energy source	1712:1727	an energy source for the bacteria	1712:1744	Taken together, these data reveal that EPS synthesis fine-tuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria.					
30659765	3	23	theme	S	696:696	arg1	biofilms					705:712	S mutans biofilms	696:712	S mutans biofilms	696:712	Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutans biofilms.					
30659765	9	24	theme	EPS	1623:1625	arg1	matrix					1627:1632	a densely packed EPS matrix	1606:1632	a densely packed EPS matrix that may impede the glucose metabolism of WSG	1606:1678	Taken together, these data reveal that EPS synthesis fine-tuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria.					
30659765	5	25	theme	increased	891:899	arg1	transcription					901:913	increased transcription	891:913	increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf	891:975	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	1	26	theme	dietary	207:213	arg1	sucrose					215:221	dietary sucrose	207:221	dietary sucrose for the synthesis of exopolysaccharides (EPS), which act as a scaffold for its biofilm and thus contribute to its cariogenic pathogenicity	207:360	The cariogenic pathogen Streptococcus mutans effectively utilizes dietary sucrose for the synthesis of exopolysaccharides (EPS), which act as a scaffold for its biofilm and thus contribute to its cariogenic pathogenicity.					
30659765	9	27	theme	source	1722:1727	arg1	lack					1704:1707	the lack	1700:1707	the lack of an energy source for the bacteria	1700:1744	Taken together, these data reveal that EPS synthesis fine-tuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria.					
30659765	2	28	theme	WSG	468:470	arg1	degradation					431:441	the degradation	427:441	the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention	427:574	Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention.					
30659765	0	29	dep	effects	83:89	arg1	aggregation					99:109	biofilm aggregation	91:109	biofilm aggregation	91:109	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	0	29	dep	effects	83:89	arg1	pathogenicity					126:138	cariogenic pathogenicity	115:138	cariogenic pathogenicity	115:138	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	0	29	dep	effects	83:89	arg1	effects					83:89	gene effects biofilm aggregation and cariogenic pathogenicity	78:138	gene effects biofilm aggregation and cariogenic pathogenicity	78:138	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	7	30	theme	mannose	1214:1220	arg1	monosaccharides					1222:1236	mannose monosaccharides	1214:1236	mannose monosaccharides	1214:1236	Furthermore, structural analysis provides new insights regarding the lack of mannose monosaccharides, especially in the WSG synthesis of the SmudexA mutants.					
30659765	9	31	theme	dexA	1585:1588	arg1	gene					1590:1593	the dexA gene	1581:1593	the dexA gene	1581:1593	Taken together, these data reveal that EPS synthesis fine-tuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria.					
30659765	10	32	theme	caries	1828:1833	arg1	management					1835:1844	dental caries management	1821:1844	dental caries management	1821:1844	These results highlight dexA targeting as a potentially effective tool in dental caries management.					
30659765	8	33	dep	biofilm	1446:1452	arg1	phenotypes					1307:1316	The biofilm phenotypes	1295:1316	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift	1295:1433	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	9	34	theme	packed	1616:1621	arg1	matrix					1627:1632	a densely packed EPS matrix	1606:1632	a densely packed EPS matrix that may impede the glucose metabolism of WSG	1606:1678	Taken together, these data reveal that EPS synthesis fine-tuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria.					
30659765	0	35	theme	glucan	28:33	arg1	synthesis					35:43	water-soluble glucan synthesis	14:43	water-soluble glucan synthesis	14:43	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	7	36	theme	mutants	1286:1292	arg1	synthesis					1261:1269	the WSG synthesis	1253:1269	the WSG synthesis of the SmudexA mutants	1253:1292	Furthermore, structural analysis provides new insights regarding the lack of mannose monosaccharides, especially in the WSG synthesis of the SmudexA mutants.					
30659765	8	37	theme	water-insoluble	1406:1420	arg1	shift					1429:1433	water-insoluble glucan shift	1406:1433	water-insoluble glucan shift	1406:1433	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	8	38	theme	reduced	1347:1353	arg1	composition					1378:1388	the reduced glucose monosaccharide composition	1343:1388	the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift	1343:1433	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	1	39	dep	pathogen	156:163	arg1	mutans					179:184	Streptococcus mutans	165:184	The cariogenic pathogen Streptococcus mutans	141:184	The cariogenic pathogen Streptococcus mutans effectively utilizes dietary sucrose for the synthesis of exopolysaccharides (EPS), which act as a scaffold for its biofilm and thus contribute to its cariogenic pathogenicity.					
30659765	0	40	theme	water-soluble	14:26	arg1	synthesis					35:43	water-soluble glucan synthesis	14:43	water-soluble glucan synthesis	14:43	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	2	41	theme	EPS	514:516	arg1	features					498:505	the structural features	483:505	the structural features of the EPS regulated by the dexAgene	483:542	Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention.					
30659765	5	42	theme	EPS	918:920	arg1	gtfD					963:966	gtfD	963:966	gtfD	963:966	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	5	42	theme	EPS	918:920	arg1	genes					940:944	EPS synthesis-related genes	918:944	EPS synthesis-related genes	918:944	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	5	42	theme	EPS	918:920	arg1	gtfB					957:960	gtfB	957:960	gtfB	957:960	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	5	42	theme	EPS	918:920	arg1	ftf					973:975	ftf	973:975	ftf	973:975	Our results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf.					
30659765	8	43	from	composition	1378:1388	arg1	WSG					1398:1400	WSG	1398:1400	WSG	1398:1400	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	8	43	from	composition	1378:1388	arg1	shift					1429:1433	water-insoluble glucan shift	1406:1433	water-insoluble glucan shift	1406:1433	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	2	44	theme	glucanase	400:408	arg1	Dextranase					363:372	Dextranase	363:372	Dextranase (Dex)	363:378	Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention.					
30659765	2	44	theme	glucanase	400:408	arg1	type					392:395	a type	390:395	a type of glucanase	390:408	Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention.					
30659765	3	45	from	characteristics	664:678	arg1	biofilms					705:712	S mutans biofilms	696:712	S mutans biofilms	696:712	Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutans biofilms.					
30659765	9	46	theme	EPS	1552:1554	arg1	fine-tuning					1566:1576	EPS synthesis fine-tuning	1552:1576	EPS synthesis fine-tuning by the dexA gene	1552:1593	Taken together, these data reveal that EPS synthesis fine-tuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria.					
30659765	2	47	theme	glucan	460:465	arg1	WSG					468:470	water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention	446:574	water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention	446:574	Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention.					
30659765	0	48	theme	synthesis	35:43	arg1	Regulation					0:9	Regulation	0:9	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.	0:139	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	3	49	theme	novel	605:609	arg1	protocols					611:619	novel protocols	605:619	novel protocols	605:619	Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutans biofilms.					
30659765	3	50	from	biofilms	705:712	arg1	glucans					683:689	glucans	683:689	glucans from S mutans biofilms	683:712	Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutans biofilms.					
30659765	3	50	from	biofilms	705:712	arg1	characteristics					664:678	the structural characteristics	649:678	the structural characteristics of glucans from S mutans biofilms	649:712	Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutans biofilms.					
30659765	4	51	from	aggregation	797:807	arg1	formation					820:828	biofilm formation	812:828	biofilm formation	812:828	In this study, we identify the role of the S mutans dexAgene in dextran-dependent aggregation in biofilm formation.					
30659765	1	52	theme	exopolysaccharides	244:261	arg1	synthesis					231:239	the synthesis	227:239	the synthesis of exopolysaccharides (EPS), which act as a scaffold for its biofilm and thus contribute to its cariogenic pathogenicity	227:360	The cariogenic pathogen Streptococcus mutans effectively utilizes dietary sucrose for the synthesis of exopolysaccharides (EPS), which act as a scaffold for its biofilm and thus contribute to its cariogenic pathogenicity.					
30659765	0	53	theme	Streptococcus	52:64	arg1	mutans					66:71	the Streptococcus mutans	48:71	the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity	48:138	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	7	54	theme	new	1179:1181	arg1	insights					1183:1190	new insights	1179:1190	new insights regarding the lack of mannose monosaccharides	1179:1236	Furthermore, structural analysis provides new insights regarding the lack of mannose monosaccharides, especially in the WSG synthesis of the SmudexA mutants.					
30659765	8	55	theme	glucose	1355:1361	arg1	composition					1378:1388	the reduced glucose monosaccharide composition	1343:1388	the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift	1343:1433	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	8	56	theme	SmudexA	1496:1502	arg1	mutants					1504:1510	the SmudexA mutants	1492:1510	the SmudexA mutants	1492:1510	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	6	57	theme	S	1073:1073	arg1	mutans					1075:1080	S mutans	1073:1080	S mutans	1073:1080	Interestingly, we reveal that inactivating the dexA gene may lead to elevated WSG synthesis in S mutans , which results in dysregulated cariogenicity in vivo.					
30659765	9	58	theme	glucose	1654:1660	arg1	metabolism					1662:1671	the glucose metabolism	1650:1671	the glucose metabolism of WSG	1650:1678	Taken together, these data reveal that EPS synthesis fine-tuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria.					
30659765	2	59	dep	WSG	468:470	arg1	received					549:556	received	549:556	have received limited attention	544:574	Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention.					
30659765	4	60	theme	dextran-dependent	779:795	arg1	aggregation					797:807	dextran-dependent aggregation	779:807	dextran-dependent aggregation in biofilm formation	779:828	In this study, we identify the role of the S mutans dexAgene in dextran-dependent aggregation in biofilm formation.					
30659765	7	61	theme	SmudexA	1278:1284	arg1	mutants					1286:1292	the SmudexA mutants	1274:1292	the SmudexA mutants	1274:1292	Furthermore, structural analysis provides new insights regarding the lack of mannose monosaccharides, especially in the WSG synthesis of the SmudexA mutants.					
30659765	8	62	theme	glucan	1422:1427	arg1	shift					1429:1433	water-insoluble glucan shift	1406:1433	water-insoluble glucan shift	1406:1433	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	4	63	theme	S	758:758	arg1	dexAgene					767:774	the S mutans dexAgene	754:774	the S mutans dexAgene	754:774	In this study, we identify the role of the S mutans dexAgene in dextran-dependent aggregation in biofilm formation.					
30659765	9	64	theme	WSG	1676:1678	arg1	metabolism					1662:1671	the glucose metabolism	1650:1671	the glucose metabolism of WSG	1650:1678	Taken together, these data reveal that EPS synthesis fine-tuning by the dexA gene results in a densely packed EPS matrix that may impede the glucose metabolism of WSG, thereby leading to the lack of an energy source for the bacteria.					
30659765	3	65	theme	structural	653:662	arg1	characteristics					664:678	the structural characteristics	649:678	the structural characteristics of glucans from S mutans biofilms	649:712	Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutans biofilms.					
30659765	4	66	theme	dexAgene	767:774	arg1	role					746:749	the role	742:749	the role of the S mutans dexAgene in dextran-dependent aggregation in biofilm formation	742:828	In this study, we identify the role of the S mutans dexAgene in dextran-dependent aggregation in biofilm formation.					
30659765	10	67	from	tool	1813:1816	arg1	management					1835:1844	dental caries management	1821:1844	dental caries management	1821:1844	These results highlight dexA targeting as a potentially effective tool in dental caries management.					
30659765	10	68	theme	effective	1803:1811	arg1	tool					1813:1816	a potentially effective tool	1789:1816	a potentially effective tool in dental caries management	1789:1844	These results highlight dexA targeting as a potentially effective tool in dental caries management.					
30659765	10	68	theme	effective	1803:1811	arg1	targeting					1776:1784	dexA targeting	1771:1784	dexA targeting	1771:1784	These results highlight dexA targeting as a potentially effective tool in dental caries management.					
30659765	6	69	from	synthesis	1060:1068	arg1	mutans					1075:1080	S mutans	1073:1080	S mutans	1073:1080	Interestingly, we reveal that inactivating the dexA gene may lead to elevated WSG synthesis in S mutans , which results in dysregulated cariogenicity in vivo.					
30659765	1	70	theme	cariogenic	145:154	arg1	pathogen					156:163	The cariogenic pathogen	141:163	The cariogenic pathogen Streptococcus mutans	141:184	The cariogenic pathogen Streptococcus mutans effectively utilizes dietary sucrose for the synthesis of exopolysaccharides (EPS), which act as a scaffold for its biofilm and thus contribute to its cariogenic pathogenicity.					
30659765	8	71	theme	dental	1439:1444	arg1	biofilm					1446:1452	the dental biofilm	1435:1452	the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants	1435:1510	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	4	72	theme	mutans	760:765	arg1	dexAgene					767:774	the S mutans dexAgene	754:774	the S mutans dexAgene	754:774	In this study, we identify the role of the S mutans dexAgene in dextran-dependent aggregation in biofilm formation.					
30659765	8	73	theme	mutants	1504:1510	arg1	incidence					1479:1487	the cariogenic incidence	1464:1487	the cariogenic incidence of the SmudexA mutants	1464:1510	The biofilm phenotypes that are associated with the reduced glucose monosaccharide composition in both WSG and water-insoluble glucan shift the dental biofilm to reduce the cariogenic incidence of the SmudexA mutants.					
30659765	6	74	theme	elevated	1047:1054	arg1	synthesis					1060:1068	elevated WSG synthesis	1047:1068	elevated WSG synthesis	1047:1068	Interestingly, we reveal that inactivating the dexA gene may lead to elevated WSG synthesis in S mutans , which results in dysregulated cariogenicity in vivo.					
30659765	6	75	theme	dexA	1025:1028	arg1	gene					1030:1033	the dexA gene	1021:1033	the dexA gene	1021:1033	Interestingly, we reveal that inactivating the dexA gene may lead to elevated WSG synthesis in S mutans , which results in dysregulated cariogenicity in vivo.					
30659765	2	76	theme	water-soluble	446:458	arg1	WSG					468:470	water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention	446:574	water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention	446:574	Dextranase (Dex), which is a type of glucanase, participates in the degradation of water-soluble glucan (WSG); however, the structural features of the EPS regulated by the dexAgene have received limited attention.					
30659765	3	77	theme	glucans	683:689	arg1	characteristics					664:678	the structural characteristics	649:678	the structural characteristics of glucans from S mutans biofilms	649:712	Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutans biofilms.					
30659765	3	78	theme	recent	581:586	arg1	studies					588:594	Our recent studies	577:594	Our recent studies	577:594	Our recent studies reported novel protocols to fractionate and analyzed the structural characteristics of glucans from S mutans biofilms.					
30659765	0	79	theme	biofilm	91:97	arg1	aggregation					99:109	biofilm aggregation	91:109	biofilm aggregation	91:109	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30659765	0	79	theme	biofilm	91:97	arg1	effects					83:89	gene effects biofilm aggregation and cariogenic pathogenicity	78:138	gene effects biofilm aggregation and cariogenic pathogenicity	78:138	Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.					
30095429	12	0	theme	NPs	1361:1363	arg1	supplement					1399:1408	a potent antioxidant nutrition supplement	1368:1408	a potent antioxidant nutrition supplement at a much lower dose	1368:1429	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	12	0	theme	NPs	1361:1363	arg1	applicability					1321:1333	the applicability	1317:1333	the applicability of tree gum stabilised Se NPs	1317:1363	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	9	1	theme	Antibacterial	1028:1040	arg1	potential					1042:1050	Antibacterial potential	1028:1050	Antibacterial potential of NPs	1028:1057	Antibacterial potential of NPs was checked with well diffusion assay.					
30095429	3	2	theme	gum	308:310	arg1	Role					300:303	Role	300:303	Role of gum on synthesis and mean particle size	300:346	Role of gum on synthesis and mean particle size was studied using ultraviolet-visible spectroscopy and dynamic light scattering.					
30095429	4	3	theme	plasmon	514:520	arg1	resonance					522:530	surface plasmon resonance	506:530	a surface plasmon resonance peak	504:535	NP generation was visualised with orange red colouration and NPs exhibited a surface plasmon resonance peak at 250 nm.					
30095429	6	4	dep	200 nm	663:668	arg1	to					660:661	to	660:661	to	660:661	NPs showed a bimodal distribution, size varied from 44.4 to 200 nm and mean particle size was 105.6 nm.					
30095429	6	5	theme	bimodal	616:622	arg1	distribution					624:635	a bimodal distribution	614:635	a bimodal distribution	614:635	NPs showed a bimodal distribution, size varied from 44.4 to 200 nm and mean particle size was 105.6 nm.					
30095429	3	6	theme	particle	334:341	arg1	size					343:346	mean particle size	329:346	mean particle size	329:346	Role of gum on synthesis and mean particle size was studied using ultraviolet-visible spectroscopy and dynamic light scattering.					
30095429	4	7	theme	surface	506:512	arg1	resonance					522:530	surface plasmon resonance	506:530	a surface plasmon resonance peak	504:535	NP generation was visualised with orange red colouration and NPs exhibited a surface plasmon resonance peak at 250 nm.					
30095429	8	8	theme	scavenging	960:969	arg1	activities					971:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities	853:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%	853:998	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	12	9	theme	tree	1338:1341	arg1	NPs					1361:1363	tree gum stabilised Se NPs	1338:1363	tree gum stabilised Se NPs	1338:1363	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	8	10	theme	gum	825:827	arg1	NPs					839:841	the gum capped Se NPs	821:841	the gum capped Se NPs	821:841	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	12	11	from	dose	1426:1429	arg1	supplement					1399:1408	a potent antioxidant nutrition supplement	1368:1408	a potent antioxidant nutrition supplement at a much lower dose	1368:1429	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	12	11	from	dose	1426:1429	arg1	applicability					1321:1333	the applicability	1317:1333	the applicability of tree gum stabilised Se NPs	1317:1363	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	12	12	theme	lower	1420:1424	arg1	dose					1426:1429	a much lower dose	1413:1429	a much lower dose	1413:1429	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	8	13	theme	2	898:898	arg1	activities					971:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities	853:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%	853:998	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	0	14	theme	Tree	0:3	arg1	gum					5:7	Tree gum	0:7	Tree gum	0:7	Tree gum stabilised selenium nanoparticles: characterisation and antioxidant activity.					
30095429	9	15	theme	NPs	1055:1057	arg1	potential					1042:1050	Antibacterial potential	1028:1050	Antibacterial potential of NPs	1028:1057	Antibacterial potential of NPs was checked with well diffusion assay.					
30095429	7	16	theme	NP	707:708	arg1	solution					710:717	NP solution	707:717	NP solution	707:717	NP solution exhibited a zeta potential of -39.9 mV, confirming the superior stability.					
30095429	4	17	theme	red	470:472	arg1	colouration					474:484	orange red colouration	463:484	orange red colouration	463:484	NP generation was visualised with orange red colouration and NPs exhibited a surface plasmon resonance peak at 250 nm.					
30095429	8	18	theme	Se	836:837	arg1	NPs					839:841	the gum capped Se NPs	821:841	the gum capped Se NPs	821:841	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	2	19	dep	renewable	233:241	arg1	non-immunogenic					255:269	non-immunogenic	255:269	non-immunogenic	255:269	Plant gum serves as a renewable, non-toxic, non-immunogenic, biopolymer based feedstock.					
30095429	2	19	dep	renewable	233:241	arg1	based					283:287	based	283:287	based	283:287	Plant gum serves as a renewable, non-toxic, non-immunogenic, biopolymer based feedstock.					
30095429	2	19	dep	renewable	233:241	arg1	non-toxic					244:252	non-toxic	244:252	non-toxic	244:252	Plant gum serves as a renewable, non-toxic, non-immunogenic, biopolymer based feedstock.					
30095429	3	20	theme	mean	329:332	arg1	size					343:346	mean particle size	329:346	mean particle size	329:346	Role of gum on synthesis and mean particle size was studied using ultraviolet-visible spectroscopy and dynamic light scattering.					
30095429	1	21	theme	Selenium	87:94	arg1	nanoparticles					96:108	Selenium nanoparticles	87:108	Selenium nanoparticles (Se NPs)	87:117	Selenium nanoparticles (Se NPs) were synthesised using sodium borohydride as a reductant and gum kondagogu as a stabiliser.					
30095429	1	21	theme	Selenium	87:94	arg1	NPs					114:116	Se NPs	111:116	Se NPs	111:116	Selenium nanoparticles (Se NPs) were synthesised using sodium borohydride as a reductant and gum kondagogu as a stabiliser.					
30095429	8	22	theme	superior	853:860	arg1	activities					971:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities	853:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%	853:998	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	6	23	theme	particle	679:686	arg1	size					688:691	mean particle size	674:691	mean particle size	674:691	NPs showed a bimodal distribution, size varied from 44.4 to 200 nm and mean particle size was 105.6 nm.					
30095429	3	24	theme	ultraviolet-visible	366:384	arg1	spectroscopy					386:397	ultraviolet-visible spectroscopy	366:397	ultraviolet-visible spectroscopy	366:397	Role of gum on synthesis and mean particle size was studied using ultraviolet-visible spectroscopy and dynamic light scattering.					
30095429	10	25	theme	inhibition	1119:1128	arg1	activity					1130:1137	growth inhibition activity	1112:1137	growth inhibition activity against Gram-positive bacteria	1112:1168	NPs exhibited growth inhibition activity against Gram-positive bacteria only.					
30095429	6	26	theme	mean	674:677	arg1	size					688:691	mean particle size	674:691	mean particle size	674:691	NPs showed a bimodal distribution, size varied from 44.4 to 200 nm and mean particle size was 105.6 nm.					
30095429	0	27	theme	selenium	20:27	arg1	characterisation					44:59	characterisation	44:59	characterisation	44:59	Tree gum stabilised selenium nanoparticles: characterisation and antioxidant activity.					
30095429	0	27	theme	selenium	20:27	arg1	activity					77:84	antioxidant activity	65:84	antioxidant activity	65:84	Tree gum stabilised selenium nanoparticles: characterisation and antioxidant activity.					
30095429	0	27	theme	selenium	20:27	arg1	nanoparticles					29:41	selenium nanoparticles	20:41	selenium nanoparticles: characterisation and antioxidant activity	20:84	Tree gum stabilised selenium nanoparticles: characterisation and antioxidant activity.					
30095429	10	28	theme	growth	1112:1117	arg1	activity					1130:1137	growth inhibition activity	1112:1137	growth inhibition activity against Gram-positive bacteria	1112:1168	NPs exhibited growth inhibition activity against Gram-positive bacteria only.					
30095429	10	29	theme	Gram-positive	1147:1159	arg1	bacteria					1161:1168	Gram-positive bacteria	1147:1168	Gram-positive bacteria	1147:1168	NPs exhibited growth inhibition activity against Gram-positive bacteria only.					
30095429	0	30	dep	nanoparticles	29:41	arg1	characterisation					44:59	characterisation	44:59	characterisation	44:59	Tree gum stabilised selenium nanoparticles: characterisation and antioxidant activity.					
30095429	0	30	dep	nanoparticles	29:41	arg1	activity					77:84	antioxidant activity	65:84	antioxidant activity	65:84	Tree gum stabilised selenium nanoparticles: characterisation and antioxidant activity.					
30095429	0	30	dep	nanoparticles	29:41	arg1	nanoparticles					29:41	selenium nanoparticles	20:41	selenium nanoparticles: characterisation and antioxidant activity	20:84	Tree gum stabilised selenium nanoparticles: characterisation and antioxidant activity.					
30095429	1	31	theme	Se	111:112	arg1	nanoparticles					96:108	Selenium nanoparticles	87:108	Selenium nanoparticles (Se NPs)	87:117	Selenium nanoparticles (Se NPs) were synthesised using sodium borohydride as a reductant and gum kondagogu as a stabiliser.					
30095429	1	31	theme	Se	111:112	arg1	NPs					114:116	Se NPs	111:116	Se NPs	111:116	Selenium nanoparticles (Se NPs) were synthesised using sodium borohydride as a reductant and gum kondagogu as a stabiliser.					
30095429	8	32	theme	1	862:862	arg1	activities					971:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities	853:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%	853:998	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	7	33	theme	superior	774:781	arg1	stability					783:791	the superior stability	770:791	the superior stability	770:791	NP solution exhibited a zeta potential of -39.9 mV, confirming the superior stability.					
30095429	12	34	theme	potent	1370:1375	arg1	supplement					1399:1408	a potent antioxidant nutrition supplement	1368:1408	a potent antioxidant nutrition supplement at a much lower dose	1368:1429	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	12	34	theme	potent	1370:1375	arg1	applicability					1321:1333	the applicability	1317:1333	the applicability of tree gum stabilised Se NPs	1317:1363	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	9	35	theme	well	1076:1079	arg1	assay					1091:1095	well diffusion assay	1076:1095	well diffusion assay	1076:1095	Antibacterial potential of NPs was checked with well diffusion assay.					
30095429	3	36	theme	dynamic	403:409	arg1	scattering					417:426	dynamic light scattering	403:426	dynamic light scattering	403:426	Role of gum on synthesis and mean particle size was studied using ultraviolet-visible spectroscopy and dynamic light scattering.					
30095429	11	37	theme	respective	1224:1233	arg1	zones					1246:1250	respective inhibition zones	1224:1250	respective inhibition zones of 6.3 and 8.6 mm	1224:1268	Bacillus subtilis and Micrococcus luteus showed respective inhibition zones of 6.3 and 8.6 mm at 12 µg.					
30095429	8	38	theme	capped	829:834	arg1	NPs					839:841	the gum capped Se NPs	821:841	the gum capped Se NPs	821:841	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	12	39	theme	stabilised	1347:1356	arg1	NPs					1361:1363	tree gum stabilised Se NPs	1338:1363	tree gum stabilised Se NPs	1338:1363	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	8	40	theme	1-diphenyl-2-picrylhydrazyle	865:892	arg1	activities					971:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities	853:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%	853:998	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	12	41	theme	nutrition	1389:1397	arg1	supplement					1399:1408	a potent antioxidant nutrition supplement	1368:1408	a potent antioxidant nutrition supplement at a much lower dose	1368:1429	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	12	41	theme	nutrition	1389:1397	arg1	applicability					1321:1333	the applicability	1317:1333	the applicability of tree gum stabilised Se NPs	1317:1363	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	3	42	theme	light	411:415	arg1	scattering					417:426	dynamic light scattering	403:426	dynamic light scattering	403:426	Role of gum on synthesis and mean particle size was studied using ultraviolet-visible spectroscopy and dynamic light scattering.					
30095429	12	43	theme	ionic	1451:1455	arg1	Se					1457:1458	ionic Se	1451:1458	ionic Se	1451:1458	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	11	44	theme	inhibition	1235:1244	arg1	zones					1246:1250	respective inhibition zones	1224:1250	respective inhibition zones of 6.3 and 8.6 mm	1224:1268	Bacillus subtilis and Micrococcus luteus showed respective inhibition zones of 6.3 and 8.6 mm at 12 µg.					
30095429	4	45	theme	orange	463:468	arg1	colouration					474:484	orange red colouration	463:484	orange red colouration	463:484	NP generation was visualised with orange red colouration and NPs exhibited a surface plasmon resonance peak at 250 nm.					
30095429	12	46	theme	gum	1343:1345	arg1	NPs					1361:1363	tree gum stabilised Se NPs	1338:1363	tree gum stabilised Se NPs	1338:1363	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	12	47	theme	Se	1358:1359	arg1	NPs					1361:1363	tree gum stabilised Se NPs	1338:1363	tree gum stabilised Se NPs	1338:1363	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	4	48	theme	resonance	522:530	arg1	peak					532:535	a surface plasmon resonance peak	504:535	a surface plasmon resonance peak	504:535	NP generation was visualised with orange red colouration and NPs exhibited a surface plasmon resonance peak at 250 nm.					
30095429	11	49	theme	8.6 mm	1263:1268	arg1	zones					1246:1250	respective inhibition zones	1224:1250	respective inhibition zones of 6.3 and 8.6 mm	1224:1268	Bacillus subtilis and Micrococcus luteus showed respective inhibition zones of 6.3 and 8.6 mm at 12 µg.					
30095429	1	50	dep	reductant	166:174	arg1	kondagogu					184:192	kondagogu	184:192	kondagogu	184:192	Selenium nanoparticles (Se NPs) were synthesised using sodium borohydride as a reductant and gum kondagogu as a stabiliser.					
30095429	5	51	theme	Formed	548:553	arg1	NPs					555:557	Formed NPs	548:557	Formed NPs	548:557	Formed NPs were amorphous, polydisperse and spherical.					
30095429	7	52	theme	-39.9 mV	749:756	arg1	potential					736:744	a zeta potential	729:744	a zeta potential of -39.9 mV	729:756	NP solution exhibited a zeta potential of -39.9 mV, confirming the superior stability.					
30095429	2	53	theme	Plant	211:215	arg1	feedstock					289:297	a renewable, non-toxic, non-immunogenic, biopolymer based feedstock	231:297	a renewable, non-toxic, non-immunogenic, biopolymer based feedstock	231:297	Plant gum serves as a renewable, non-toxic, non-immunogenic, biopolymer based feedstock.					
30095429	2	53	theme	Plant	211:215	arg1	gum					217:219	Plant gum	211:219	Plant gum	211:219	Plant gum serves as a renewable, non-toxic, non-immunogenic, biopolymer based feedstock.					
30095429	8	54	theme	%	998:998	arg1	activities					971:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities	853:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%	853:998	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	11	55	theme	6.3	1255:1257	arg1	zones					1246:1250	respective inhibition zones	1224:1250	respective inhibition zones of 6.3 and 8.6 mm	1224:1268	Bacillus subtilis and Micrococcus luteus showed respective inhibition zones of 6.3 and 8.6 mm at 12 µg.					
30095429	12	56	theme	present	1290:1296	arg1	study					1298:1302	the present study	1286:1302	the present study	1286:1302	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	12	57	theme	antioxidant	1377:1387	arg1	supplement					1399:1408	a potent antioxidant nutrition supplement	1368:1408	a potent antioxidant nutrition supplement at a much lower dose	1368:1429	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	12	57	theme	antioxidant	1377:1387	arg1	applicability					1321:1333	the applicability	1317:1333	the applicability of tree gum stabilised Se NPs	1317:1363	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	12	58	with	comparison	1435:1444	arg1	Se					1457:1458	ionic Se	1451:1458	ionic Se	1451:1458	Thus, the present study demonstrates the applicability of tree gum stabilised Se NPs as a potent antioxidant nutrition supplement at a much lower dose, in comparison with ionic Se.					
30095429	9	59	theme	diffusion	1081:1089	arg1	assay					1091:1095	well diffusion assay	1076:1095	well diffusion assay	1076:1095	Antibacterial potential of NPs was checked with well diffusion assay.					
30095429	4	60	theme	NP	429:430	arg1	generation					432:441	NP generation	429:441	NP generation	429:441	NP generation was visualised with orange red colouration and NPs exhibited a surface plasmon resonance peak at 250 nm.					
30095429	2	61	theme	renewable	233:241	arg1	feedstock					289:297	a renewable, non-toxic, non-immunogenic, biopolymer based feedstock	231:297	a renewable, non-toxic, non-immunogenic, biopolymer based feedstock	231:297	Plant gum serves as a renewable, non-toxic, non-immunogenic, biopolymer based feedstock.					
30095429	2	61	theme	renewable	233:241	arg1	gum					217:219	Plant gum	211:219	Plant gum	211:219	Plant gum serves as a renewable, non-toxic, non-immunogenic, biopolymer based feedstock.					
30095429	8	62	theme	3-ethylbenzthinzoline-6-sulphonic	913:945	arg1	acid					947:950	3-ethylbenzthinzoline-6-sulphonic acid	913:950	3-ethylbenzthinzoline-6-sulphonic acid	913:950	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	8	62	theme	3-ethylbenzthinzoline-6-sulphonic	913:945	arg1	2					898:898	2	898:898	2	898:898	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	1	63	theme	sodium	142:147	arg1	borohydride					149:159	sodium borohydride	142:159	sodium borohydride	142:159	Selenium nanoparticles (Se NPs) were synthesised using sodium borohydride as a reductant and gum kondagogu as a stabiliser.					
30095429	1	63	theme	sodium	142:147	arg1	reductant					166:174	a reductant	164:174	a reductant	164:174	Selenium nanoparticles (Se NPs) were synthesised using sodium borohydride as a reductant and gum kondagogu as a stabiliser.					
30095429	1	63	theme	sodium	142:147	arg1	stabiliser					199:208	a stabiliser	197:208	a stabiliser	197:208	Selenium nanoparticles (Se NPs) were synthesised using sodium borohydride as a reductant and gum kondagogu as a stabiliser.					
30095429	8	64	theme	ionic	811:815	arg1	Se					817:818	ionic Se	811:818	ionic Se	811:818	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	0	65	theme	antioxidant	65:75	arg1	activity					77:84	antioxidant activity	65:84	antioxidant activity	65:84	Tree gum stabilised selenium nanoparticles: characterisation and antioxidant activity.					
30095429	0	65	theme	antioxidant	65:75	arg1	nanoparticles					29:41	selenium nanoparticles	20:41	selenium nanoparticles: characterisation and antioxidant activity	20:84	Tree gum stabilised selenium nanoparticles: characterisation and antioxidant activity.					
30095429	7	66	theme	zeta	731:734	arg1	potential					736:744	a zeta potential	729:744	a zeta potential of -39.9 mV	729:756	NP solution exhibited a zeta potential of -39.9 mV, confirming the superior stability.					
30095429	8	67	theme	radial	953:958	arg1	activities					971:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities	853:980	superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%	853:998	In comparison to ionic Se, the gum capped Se NPs exhibited superior 1, 1-diphenyl-2-picrylhydrazyle and 2, 2-azinobis-(3-ethylbenzthinzoline-6-sulphonic acid) radial scavenging activities of 73.2 and 92.2%, respectively, at 25 µg/ml.					
30095429	3	68	from	Role	300:303	arg1	synthesis					315:323	synthesis	315:323	synthesis	315:323	Role of gum on synthesis and mean particle size was studied using ultraviolet-visible spectroscopy and dynamic light scattering.					
30095429	3	68	from	Role	300:303	arg1	size					343:346	mean particle size	329:346	mean particle size	329:346	Role of gum on synthesis and mean particle size was studied using ultraviolet-visible spectroscopy and dynamic light scattering.					
31102805	1	0	theme	remarkable	397:406	arg1	bioavailability					416:430	remarkable in-vivo bioavailability	397:430	remarkable in-vivo bioavailability	397:430	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	7	1	theme	Microscope	1188:1197	arg1	analysis					1199:1206	Scanning Electron Microscope analysis	1170:1206	Scanning Electron Microscope analysis	1170:1206	Scanning Electron Microscope analysis revealed the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite.					
31102805	8	2	theme	MG-63	1445:1449	arg1	erythrocytes					1422:1433	the erythrocytes	1418:1433	the erythrocytes	1418:1433	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	8	2	theme	MG-63	1445:1449	arg1	lines					1456:1460	MG-63 cell lines	1445:1460	MG-63 cell lines	1445:1460	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	4	3	with	modification	794:805	arg1	system					858:863	the polymeric-ceramic system	836:863	the polymeric-ceramic system	836:863	The chemical modification and interaction of drug with the polymeric-ceramic system were characterised by Fourier Transform Infrared spectroscopy (FT-IR).					
31102805	1	4	theme	in-vivo	408:414	arg1	bioavailability					416:430	remarkable in-vivo bioavailability	397:430	remarkable in-vivo bioavailability	397:430	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	9	5	theme	osteoblastic	1812:1823	arg1	cells					1830:1834	osteoblastic bone cells	1812:1834	osteoblastic bone cells	1812:1834	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	1	6	theme	high	313:316	arg1	capability					331:340	their high drug loading capability	307:340	their high drug loading capability	307:340	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	3	7	theme	-Car-MA-INH/NHAP/RF	745:763	arg1	nanocomposites					765:778	κ -Car-MA-INH/NHAP/RF nanocomposites	743:778	κ -Car-MA-INH/NHAP/RF nanocomposites	743:778	The polymeric system was cross linked with nanohydroxyapatite (NHAP) via electrostatic interaction followed by the addition of rifampicin (RF) and loaded to fabricate κ -Car-MA-INH/NHAP/RF nanocomposites.					
31102805	7	8	theme	Scanning	1170:1177	arg1	analysis					1199:1206	Scanning Electron Microscope analysis	1170:1206	Scanning Electron Microscope analysis	1170:1206	Scanning Electron Microscope analysis revealed the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite.					
31102805	1	9	with	strength	383:390	arg1	bioavailability					416:430	remarkable in-vivo bioavailability	397:430	remarkable in-vivo bioavailability	397:430	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	4	10	theme	polymeric-ceramic	840:856	arg1	system					858:863	the polymeric-ceramic system	836:863	the polymeric-ceramic system	836:863	The chemical modification and interaction of drug with the polymeric-ceramic system were characterised by Fourier Transform Infrared spectroscopy (FT-IR).					
31102805	4	11	theme	drug	826:829	arg1	interaction					811:821	interaction	811:821	interaction of drug with the polymeric-ceramic system	811:863	The chemical modification and interaction of drug with the polymeric-ceramic system were characterised by Fourier Transform Infrared spectroscopy (FT-IR).					
31102805	4	11	theme	drug	826:829	arg1	modification					794:805	The chemical modification	781:805	The chemical modification	781:805	The chemical modification and interaction of drug with the polymeric-ceramic system were characterised by Fourier Transform Infrared spectroscopy (FT-IR).					
31102805	1	12	theme	drug	318:321	arg1	capability					331:340	their high drug loading capability	307:340	their high drug loading capability	307:340	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	0	13	theme	tissue	126:131	arg1	regeneration					133:144	tissue regeneration	126:144	tissue regeneration	126:144	Fabrication of bioactive rifampicin loaded κ-Car-MA-INH/Nano hydroxyapatite composite for tuberculosis osteomyelitis infected tissue regeneration.					
31102805	9	14	theme	drug	1725:1728	arg1	nanocomposites					1739:1752	long-acting drug delivery nanocomposites	1713:1752	long-acting drug delivery nanocomposites	1713:1752	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	7	15	theme	Electron	1179:1186	arg1	analysis					1199:1206	Scanning Electron Microscope analysis	1170:1206	Scanning Electron Microscope analysis	1170:1206	Scanning Electron Microscope analysis revealed the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite.					
31102805	7	16	theme	structural	1221:1230	arg1	deformation					1232:1242	the structural deformation	1217:1242	the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite	1217:1332	Scanning Electron Microscope analysis revealed the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite.					
31102805	8	17	theme	cell	1451:1454	arg1	erythrocytes					1422:1433	the erythrocytes	1418:1433	the erythrocytes	1418:1433	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	8	17	theme	cell	1451:1454	arg1	lines					1456:1460	MG-63 cell lines	1445:1460	MG-63 cell lines	1445:1460	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	1	18	theme	loading	323:329	arg1	capability					331:340	their high drug loading capability	307:340	their high drug loading capability	307:340	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	9	19	theme	modified	1598:1605	arg1	delivery					1612:1619	our modified drug delivery	1594:1619	our modified drug delivery	1594:1619	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	9	20	theme	bone	1825:1828	arg1	cells					1830:1834	osteoblastic bone cells	1812:1834	osteoblastic bone cells	1812:1834	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	6	21	theme	INH	1144:1146	arg1	INH					1144:1146	INH	1144:1146	INH	1144:1146	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	6	21	theme	INH	1144:1146	arg1	RF					1130:1131	RF	1130:1131	RF	1130:1131	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	6	21	theme	INH	1144:1146	arg1	%					1139:1139	82%	1137:1139	82% of INH	1137:1146	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	6	21	theme	INH	1144:1146	arg1	%					1125:1125	76%	1123:1125	76% of RF	1123:1131	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	1	22	theme	vital	216:220	arg1	polymers					161:168	Biocompatible polymers	147:168	Biocompatible polymers	147:168	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	1	22	theme	vital	216:220	arg1	materials					182:190	ceramic materials	174:190	ceramic materials	174:190	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	1	22	theme	vital	216:220	arg1	components					222:231	vital components	216:231	vital components	216:231	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	7	23	theme	pneumoniae	1284:1293	arg1	deformation					1232:1242	the structural deformation	1217:1242	the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite	1217:1332	Scanning Electron Microscope analysis revealed the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite.					
31102805	2	24	theme	isoniazid	545:553	arg1	κ-Car-MA-INH					561:572	κ-Car-MA-INH	561:572	κ-Car-MA-INH	561:572	In the present work, initially we designed κ-carrageenan grafted with maleic anhydride and then reacted it with isoniazid drug (κ-Car-MA-INH).					
31102805	2	24	theme	isoniazid	545:553	arg1	drug					555:558	isoniazid drug	545:558	isoniazid drug (κ-Car-MA-INH)	545:573	In the present work, initially we designed κ-carrageenan grafted with maleic anhydride and then reacted it with isoniazid drug (κ-Car-MA-INH).					
31102805	9	25	theme	cells	1830:1834	arg1	growth					1802:1807	the growth	1798:1807	the growth of osteoblastic bone cells	1798:1834	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	4	26	dep	Fourier	887:893	arg1	Transform					895:903	Transform	895:903	Transform Infrared spectroscopy (FT-IR)	895:933	The chemical modification and interaction of drug with the polymeric-ceramic system were characterised by Fourier Transform Infrared spectroscopy (FT-IR).					
31102805	7	27	theme	aureus	1262:1267	arg1	deformation					1232:1242	the structural deformation	1217:1242	the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite	1217:1332	Scanning Electron Microscope analysis revealed the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite.					
31102805	9	28	with	nanocomposites	1739:1752	arg1	drugs					1783:1787	hydrophobic drugs	1771:1787	hydrophobic drugs inducing the growth of osteoblastic bone cells	1771:1834	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	9	29	theme	drug	1652:1655	arg1	bioavailability					1657:1671	higher drug bioavailability	1645:1671	higher drug bioavailability	1645:1671	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	1	30	with	release	353:359	arg1	bioavailability					416:430	remarkable in-vivo bioavailability	397:430	remarkable in-vivo bioavailability	397:430	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	3	31	theme	polymeric	580:588	arg1	system					590:595	The polymeric system	576:595	The polymeric system	576:595	The polymeric system was cross linked with nanohydroxyapatite (NHAP) via electrostatic interaction followed by the addition of rifampicin (RF) and loaded to fabricate κ -Car-MA-INH/NHAP/RF nanocomposites.					
31102805	6	32	theme	drug	1057:1060	arg1	studies					1070:1076	The in vitro drug release studies	1044:1076	The in vitro drug release studies	1044:1076	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	3	33	theme	κ	743:743	arg1	nanocomposites					765:778	κ -Car-MA-INH/NHAP/RF nanocomposites	743:778	κ -Car-MA-INH/NHAP/RF nanocomposites	743:778	The polymeric system was cross linked with nanohydroxyapatite (NHAP) via electrostatic interaction followed by the addition of rifampicin (RF) and loaded to fabricate κ -Car-MA-INH/NHAP/RF nanocomposites.					
31102805	9	34	theme	hydrophobic	1771:1781	arg1	drugs					1783:1787	hydrophobic drugs	1771:1787	hydrophobic drugs inducing the growth of osteoblastic bone cells	1771:1834	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	1	35	with	capability	331:340	arg1	bioavailability					416:430	remarkable in-vivo bioavailability	397:430	remarkable in-vivo bioavailability	397:430	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	5	36	theme	κ	962:962	arg1	nanocomposite					984:996	the κ -Car-MA-INH/NHAP/RF nanocomposite	958:996	the κ -Car-MA-INH/NHAP/RF nanocomposite	958:996	The zeta potential of the κ -Car-MA-INH/NHAP/RF nanocomposite was observed to be -20.04 mV using Zetasizer.					
31102805	0	37	theme	rifampicin	25:34	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of bioactive rifampicin	0:34	Fabrication of bioactive rifampicin loaded κ-Car-MA-INH/Nano hydroxyapatite composite for tuberculosis osteomyelitis infected tissue regeneration.					
31102805	6	38	theme	release	1062:1068	arg1	studies					1070:1076	The in vitro drug release studies	1044:1076	The in vitro drug release studies	1044:1076	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	8	39	dep	biocompatible	1494:1506	arg1	non-cytotoxic					1509:1521	non-cytotoxic	1509:1521	non-cytotoxic	1509:1521	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	7	40	with	treatment	1300:1308	arg1	nanocomposite					1320:1332	this nanocomposite	1315:1332	this nanocomposite	1315:1332	Scanning Electron Microscope analysis revealed the structural deformation of Staphylococcus aureus and Klebsiella pneumoniae upon treatment with this nanocomposite.					
31102805	9	41	theme	long-acting	1713:1723	arg1	nanocomposites					1739:1752	long-acting drug delivery nanocomposites	1713:1752	long-acting drug delivery nanocomposites	1713:1752	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	3	42	attach	linked	607:612	arg3	interaction					663:673	electrostatic interaction	649:673	electrostatic interaction followed by the addition of rifampicin (RF)	649:717	The polymeric system was cross linked with nanohydroxyapatite (NHAP) via electrostatic interaction followed by the addition of rifampicin (RF) and loaded to fabricate κ -Car-MA-INH/NHAP/RF nanocomposites.					
31102805	1	43	theme	drug	246:249	arg1	delivery					251:258	drug delivery	246:258	drug delivery	246:258	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	0	44	theme	bioactive	15:23	arg1	rifampicin					25:34	bioactive rifampicin	15:34	bioactive rifampicin	15:34	Fabrication of bioactive rifampicin loaded κ-Car-MA-INH/Nano hydroxyapatite composite for tuberculosis osteomyelitis infected tissue regeneration.					
31102805	1	45	theme	sustained	343:351	arg1	release					353:359	sustained release	343:359	sustained release	343:359	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	1	46	theme	Biocompatible	147:159	arg1	polymers					161:168	Biocompatible polymers	147:168	Biocompatible polymers	147:168	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	1	46	theme	Biocompatible	147:159	arg1	materials					182:190	ceramic materials	174:190	ceramic materials	174:190	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	1	46	theme	Biocompatible	147:159	arg1	components					222:231	vital components	216:231	vital components	216:231	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	5	47	theme	-Car-MA-INH/NHAP/RF	964:982	arg1	nanocomposite					984:996	the κ -Car-MA-INH/NHAP/RF nanocomposite	958:996	the κ -Car-MA-INH/NHAP/RF nanocomposite	958:996	The zeta potential of the κ -Car-MA-INH/NHAP/RF nanocomposite was observed to be -20.04 mV using Zetasizer.					
31102805	1	48	theme	delivery	251:258	arg1	applications					283:294	drug delivery and tissue engineering applications	246:294	drug delivery and tissue engineering applications	246:294	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	0	49	theme	κ-Car-MA-INH/Nano	43:59	arg1	composite					76:84	κ-Car-MA-INH/Nano hydroxyapatite composite	43:84	κ-Car-MA-INH/Nano hydroxyapatite composite	43:84	Fabrication of bioactive rifampicin loaded κ-Car-MA-INH/Nano hydroxyapatite composite for tuberculosis osteomyelitis infected tissue regeneration.					
31102805	9	50	theme	drug	1607:1610	arg1	delivery					1612:1619	our modified drug delivery	1594:1619	our modified drug delivery	1594:1619	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	9	51	theme	delivery	1730:1737	arg1	nanocomposites					1739:1752	long-acting drug delivery nanocomposites	1713:1752	long-acting drug delivery nanocomposites	1713:1752	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	8	52	theme	ex-vivo	1344:1350	arg1	studies					1352:1358	ex-vivo studies	1344:1358	ex-vivo studies combined with physio-chemical characterization methods	1344:1413	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	8	53	theme	characterization	1390:1405	arg1	methods					1407:1413	physio-chemical characterization methods	1374:1413	physio-chemical characterization methods	1374:1413	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	6	54	theme	in	1048:1049	arg1	studies					1070:1076	The in vitro drug release studies	1044:1076	The in vitro drug release studies	1044:1076	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	3	55	theme	rifampicin	703:712	arg1	addition					691:698	the addition	687:698	the addition of rifampicin (RF)	687:717	The polymeric system was cross linked with nanohydroxyapatite (NHAP) via electrostatic interaction followed by the addition of rifampicin (RF) and loaded to fabricate κ -Car-MA-INH/NHAP/RF nanocomposites.					
31102805	4	56	theme	Infrared	905:912	arg1	spectroscopy					914:925	Infrared spectroscopy	905:925	Infrared spectroscopy (FT-IR)	905:933	The chemical modification and interaction of drug with the polymeric-ceramic system were characterised by Fourier Transform Infrared spectroscopy (FT-IR).					
31102805	4	56	theme	Infrared	905:912	arg1	FT-IR					928:932	FT-IR	928:932	FT-IR	928:932	The chemical modification and interaction of drug with the polymeric-ceramic system were characterised by Fourier Transform Infrared spectroscopy (FT-IR).					
31102805	1	57	theme	tissue	264:269	arg1	engineering					271:281	tissue engineering	264:281	tissue engineering	264:281	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	1	58	theme	higher	365:370	arg1	strength					383:390	higher mechanical strength	365:390	higher mechanical strength	365:390	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	6	59	theme	RF	1130:1131	arg1	INH					1144:1146	INH	1144:1146	INH	1144:1146	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	6	59	theme	RF	1130:1131	arg1	RF					1130:1131	RF	1130:1131	RF	1130:1131	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	6	59	theme	RF	1130:1131	arg1	%					1139:1139	82%	1137:1139	82% of INH	1137:1146	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	6	59	theme	RF	1130:1131	arg1	%					1125:1125	76%	1123:1125	76% of RF	1123:1131	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	8	60	theme	significant	1565:1575	arg1	hemolysis					1577:1585	less significant hemolysis	1560:1585	less significant hemolysis	1560:1585	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	4	61	theme	chemical	785:792	arg1	modification					794:805	The chemical modification	781:805	The chemical modification	781:805	The chemical modification and interaction of drug with the polymeric-ceramic system were characterised by Fourier Transform Infrared spectroscopy (FT-IR).					
31102805	0	62	theme	hydroxyapatite	61:74	arg1	composite					76:84	κ-Car-MA-INH/Nano hydroxyapatite composite	43:84	κ-Car-MA-INH/Nano hydroxyapatite composite	43:84	Fabrication of bioactive rifampicin loaded κ-Car-MA-INH/Nano hydroxyapatite composite for tuberculosis osteomyelitis infected tissue regeneration.					
31102805	1	63	theme	mechanical	372:381	arg1	strength					383:390	higher mechanical strength	365:390	higher mechanical strength	365:390	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	3	64	theme	electrostatic	649:661	arg1	interaction					663:673	electrostatic interaction	649:673	electrostatic interaction followed by the addition of rifampicin (RF)	649:717	The polymeric system was cross linked with nanohydroxyapatite (NHAP) via electrostatic interaction followed by the addition of rifampicin (RF) and loaded to fabricate κ -Car-MA-INH/NHAP/RF nanocomposites.					
31102805	9	65	dep	nanocomposites	1621:1634	arg1	afforded					1636:1643	afforded	1636:1643	nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells	1621:1834	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	6	66	dep	in	1048:1049	arg1	vitro					1051:1055	vitro	1051:1055	vitro	1051:1055	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	4	67	with	interaction	811:821	arg1	system					858:863	the polymeric-ceramic system	836:863	the polymeric-ceramic system	836:863	The chemical modification and interaction of drug with the polymeric-ceramic system were characterised by Fourier Transform Infrared spectroscopy (FT-IR).					
31102805	1	68	theme	ceramic	174:180	arg1	polymers					161:168	Biocompatible polymers	147:168	Biocompatible polymers	147:168	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	1	68	theme	ceramic	174:180	arg1	materials					182:190	ceramic materials	174:190	ceramic materials	174:190	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	1	68	theme	ceramic	174:180	arg1	components					222:231	vital components	216:231	vital components	216:231	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	1	69	theme	engineering	271:281	arg1	applications					283:294	drug delivery and tissue engineering applications	246:294	drug delivery and tissue engineering applications	246:294	Biocompatible polymers and ceramic materials have been identified as vital components to fabricate drug delivery and tissue engineering applications because of their high drug loading capability, sustained release and higher mechanical strength with remarkable in-vivo bioavailability.					
31102805	8	70	theme	cell	1536:1539	arg1	proliferation					1541:1553	cell proliferation	1536:1553	cell proliferation	1536:1553	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	6	71	from	pH	1162:1163	arg1	12 days					1151:1157	12 days	1151:1157	12 days at pH 5.5	1151:1167	The in vitro drug release studies demonstrated that the nanocomposite releases 76% of RF and 82% of INH in 12 days at pH 5.5.					
31102805	9	72	theme	higher	1645:1650	arg1	bioavailability					1657:1671	higher drug bioavailability	1645:1671	higher drug bioavailability	1645:1671	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	3	73	dep	linked	607:612	arg1	cross					601:605	cross	601:605	cross	601:605	The polymeric system was cross linked with nanohydroxyapatite (NHAP) via electrostatic interaction followed by the addition of rifampicin (RF) and loaded to fabricate κ -Car-MA-INH/NHAP/RF nanocomposites.					
31102805	0	74	theme	tuberculosis	90:101	arg1	osteomyelitis					103:115	tuberculosis osteomyelitis	90:115	tuberculosis osteomyelitis infected tissue regeneration	90:144	Fabrication of bioactive rifampicin loaded κ-Car-MA-INH/Nano hydroxyapatite composite for tuberculosis osteomyelitis infected tissue regeneration.					
31102805	5	75	theme	zeta	940:943	arg1	-20.04 mV					1017:1025	-20.04 mV	1017:1025	-20.04 mV using Zetasizer	1017:1041	The zeta potential of the κ -Car-MA-INH/NHAP/RF nanocomposite was observed to be -20.04 mV using Zetasizer.					
31102805	5	75	theme	zeta	940:943	arg1	potential					945:953	The zeta potential	936:953	The zeta potential of the κ -Car-MA-INH/NHAP/RF nanocomposite	936:996	The zeta potential of the κ -Car-MA-INH/NHAP/RF nanocomposite was observed to be -20.04 mV using Zetasizer.					
31102805	8	76	theme	physio-chemical	1374:1388	arg1	methods					1407:1413	physio-chemical characterization methods	1374:1413	physio-chemical characterization methods	1374:1413	By using ex-vivo studies combined with physio-chemical characterization methods on the erythrocytes, L929 and MG-63 cell lines, this composite was found to be biocompatible, non-cytotoxic and inducing cell proliferation with less significant hemolysis.					
31102805	2	77	theme	present	440:446	arg1	work					448:451	the present work	436:451	the present work	436:451	In the present work, initially we designed κ-carrageenan grafted with maleic anhydride and then reacted it with isoniazid drug (κ-Car-MA-INH).					
31102805	9	78	theme	large	1678:1682	arg1	potential					1684:1692	large potential	1678:1692	large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells	1678:1834	Thus, our modified drug delivery nanocomposites afforded higher drug bioavailability with large potential for fabrication as long-acting drug delivery nanocomposites, especially with hydrophobic drugs inducing the growth of osteoblastic bone cells.					
31102805	2	79	theme	maleic	503:508	arg1	anhydride					510:518	maleic anhydride	503:518	maleic anhydride	503:518	In the present work, initially we designed κ-carrageenan grafted with maleic anhydride and then reacted it with isoniazid drug (κ-Car-MA-INH).					
31102805	5	80	theme	nanocomposite	984:996	arg1	-20.04 mV					1017:1025	-20.04 mV	1017:1025	-20.04 mV using Zetasizer	1017:1041	The zeta potential of the κ -Car-MA-INH/NHAP/RF nanocomposite was observed to be -20.04 mV using Zetasizer.					
31102805	5	80	theme	nanocomposite	984:996	arg1	potential					945:953	The zeta potential	936:953	The zeta potential of the κ -Car-MA-INH/NHAP/RF nanocomposite	936:996	The zeta potential of the κ -Car-MA-INH/NHAP/RF nanocomposite was observed to be -20.04 mV using Zetasizer.					
30599244	14	0	theme	calcium	2381:2387	arg1	hydrogels					2398:2406	calcium alginate hydrogels	2381:2406	calcium alginate hydrogels	2381:2406	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	5	1	theme	Co2+-Ca2+/Gauze/SA	666:683	arg1	dressings					705:713	Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings	666:713	Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings	666:713	After drying, SA solution was added to ionized gauze and Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings were formed easily.					
30599244	5	2	theme	ionized	648:654	arg1	gauze					656:660	ionized gauze	648:660	ionized gauze	648:660	After drying, SA solution was added to ionized gauze and Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings were formed easily.					
30599244	6	3	theme	uniform	826:832	arg1	properties					814:823	strong mechanical properties	796:823	strong mechanical properties	796:823	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	3	theme	uniform	826:832	arg1	dispersion					834:843	uniform dispersion	826:843	uniform dispersion	826:843	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	14	4	theme	property	2360:2367	arg1	capacity					2369:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity	2284:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels	2284:2406	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	2	5	from	increase	231:238	arg1	complexity					247:256	complexity	247:256	complexity	247:256	The multifunctionality often leads to an increase in the complexity and difficulty in dressing preparation.					
30599244	2	5	from	increase	231:238	arg1	difficulty					262:271	difficulty	262:271	difficulty	262:271	The multifunctionality often leads to an increase in the complexity and difficulty in dressing preparation.					
30599244	16	6	theme	wound	2630:2634	arg1	repair					2636:2641	skin wound repair duo	2625:2645	skin wound repair duo	2625:2645	Thus, we believed that the composite dressings can be widely applied in skin wound repair duo to their facile preparation method and good therapeutic effect.					
30599244	16	7	theme	composite	2580:2588	arg1	dressings					2590:2598	the composite dressings	2576:2598	the composite dressings	2576:2598	Thus, we believed that the composite dressings can be widely applied in skin wound repair duo to their facile preparation method and good therapeutic effect.					
30599244	1	8	with	dressings	90:98	arg1	functions					114:122	multiple functions	105:122	multiple functions	105:122	Wound dressings with multiple functions are required to meet the complexity of the wound healing process.					
30599244	6	9	theme	strong	796:801	arg1	properties					814:823	strong mechanical properties	796:823	strong mechanical properties	796:823	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	9	theme	strong	796:801	arg1	dispersion					834:843	uniform dispersion	826:843	uniform dispersion	826:843	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	9	theme	strong	796:801	arg1	release					859:865	sustained release	849:865	sustained release of Ca2+ and Co2+	849:882	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	4	10	with	solutions	546:554	arg1	concentration					571:583	different concentration	561:583	different concentration	561:583	Firstly, mixed Ca2+/Co2+ ion solutions with different concentration were applied to gauzes.					
30599244	8	11	theme	composite	1285:1293	arg1	dressings					1295:1303	Co2+0.25-Ca2+4 GSA composite dressings	1266:1303	Co2+0.25-Ca2+4 GSA composite dressings	1266:1303	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	14	12	theme	moisture	2324:2331	arg1	maintenance					2333:2343	moisture maintenance	2324:2343	moisture maintenance	2324:2343	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	11	13	theme	clinical	1915:1922	arg1	requirements					1924:1935	complex clinical requirements	1907:1935	complex clinical requirements	1907:1935	STATEMENT OF SIGNIFICANCE: Wound dressings with integrated functionalities are required to meet complex clinical requirements.					
30599244	7	14	theme	overall	1089:1095	arg1	effect					1097:1102	the best overall effect	1080:1102	the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity	1080:1220	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	8	15	theme	In	1223:1224	arg1	healing					1237:1243	In vivo wound healing	1223:1243	In vivo wound healing assays	1223:1250	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	14	16	theme	absorption	2312:2321	arg1	capacity					2369:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity	2284:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels	2284:2406	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	8	17	theme	wound	1231:1235	arg1	healing					1237:1243	In vivo wound healing	1223:1243	In vivo wound healing assays	1223:1250	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	14	18	theme	wound	2298:2302	arg1	absorption					2312:2321	the effective wound exudate absorption	2284:2321	the effective wound exudate absorption	2284:2321	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	3	19	used	used	327:330	arg2	we					324:325	we	324:325	we	324:325	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	7	20	theme	composite	1049:1057	arg1	Ca2+					1024:1027	4 g/L Ca2+	1018:1027	4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings)	1018:1068	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	7	20	theme	composite	1049:1057	arg1	dressings					1059:1067	Co2+0.25-Ca2+4 GSA composite dressings	1030:1067	Co2+0.25-Ca2+4 GSA composite dressings	1030:1067	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	15	21	theme	bacteria-infected	2520:2536	arg1	healing					2544:2550	bacteria-infected wound healing	2520:2550	bacteria-infected wound healing	2520:2550	These functions all together promote bacteria-infected wound healing.					
30599244	7	22	theme	0.25 g/L	1000:1007	arg1	Co2+					1009:1012	0.25 g/L Co2+	1000:1012	0.25 g/L Co2+	1000:1012	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	8	23	theme	full-thickness	1505:1518	arg1	healing					1531:1537	full-thickness skin wound healing	1505:1537	full-thickness skin wound healing	1505:1537	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	24	theme	Hypoxia-inducible	1349:1365	arg1	expression					1477:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	24	theme	Hypoxia-inducible	1349:1365	arg1	HIF-1α					1378:1383	HIF-1α	1378:1383	HIF-1α	1378:1383	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	24	theme	Hypoxia-inducible	1349:1365	arg1	factor					1415:1420	vascular endothelial growth factor	1387:1420	vascular endothelial growth factor (VEGF)	1387:1427	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	24	theme	Hypoxia-inducible	1349:1365	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	2	25	theme	dressing	276:283	arg1	preparation					285:295	dressing preparation	276:295	dressing preparation	276:295	The multifunctionality often leads to an increase in the complexity and difficulty in dressing preparation.					
30599244	8	26	theme	wound	1525:1529	arg1	healing					1531:1537	full-thickness skin wound healing	1505:1537	full-thickness skin wound healing	1505:1537	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	9	27	dep	macrophages	1632:1642	arg1	deposition					1696:1705	dense collagen deposition	1681:1705	dense collagen deposition	1681:1705	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	9	27	dep	macrophages	1632:1642	arg1	macrophages					1632:1642	macrophages	1632:1642	macrophages regulation, re-epithelialization and dense collagen deposition	1632:1705	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	9	27	dep	macrophages	1632:1642	arg1	re-epithelialization					1656:1675	re-epithelialization	1656:1675	re-epithelialization	1656:1675	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	9	27	dep	macrophages	1632:1642	arg1	regulation					1644:1653	regulation	1644:1653	regulation	1644:1653	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	11	28	theme	Wound	1838:1842	arg1	dressings					1844:1852	SIGNIFICANCE: Wound dressings	1824:1852	SIGNIFICANCE: Wound dressings with integrated functionalities	1824:1884	STATEMENT OF SIGNIFICANCE: Wound dressings with integrated functionalities are required to meet complex clinical requirements.					
30599244	1	29	theme	wound	167:171	arg1	process					181:187	the wound healing process	163:187	the wound healing process	163:187	Wound dressings with multiple functions are required to meet the complexity of the wound healing process.					
30599244	7	30	theme	above	958:962	arg1	advantages					964:973	the above advantages	954:973	the above advantages	954:973	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	1	31	theme	process	181:187	arg1	complexity					149:158	the complexity	145:158	the complexity of the wound healing process	145:187	Wound dressings with multiple functions are required to meet the complexity of the wound healing process.					
30599244	16	32	theme	facile	2656:2661	arg1	method					2675:2680	their facile preparation method	2650:2680	their facile preparation method	2650:2680	Thus, we believed that the composite dressings can be widely applied in skin wound repair duo to their facile preparation method and good therapeutic effect.					
30599244	8	33	theme	bacterial-infected	1548:1565	arg1	model					1573:1577	mouse bacterial-infected wound model	1542:1577	mouse bacterial-infected wound model	1542:1577	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	14	34	theme	gauze	2273:2277	arg1	strength					2261:2268	the mechanical strength	2246:2268	the mechanical strength of gauze	2246:2277	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	8	35	theme	vascular	1387:1394	arg1	VEGF					1423:1426	VEGF	1423:1426	VEGF	1423:1426	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	35	theme	vascular	1387:1394	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	35	theme	vascular	1387:1394	arg1	factor					1415:1420	vascular endothelial growth factor	1387:1420	vascular endothelial growth factor (VEGF)	1387:1427	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	14	36	theme	antimicrobial	2452:2464	arg1	effects					2466:2472	the antimicrobial effects	2448:2472	the antimicrobial effects of Co2+	2448:2480	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	3	37	theme	fabrication	357:367	arg1	process					369:375	fabrication process	357:375	fabrication process	357:375	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	14	38	contain	had	2280:2282	arg2	capacity					2369:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity	2284:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels	2284:2406	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	14	38	contain	had	2280:2282	arg1	dressings					2226:2234	The composite dressings	2212:2234	The composite dressings	2212:2234	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	8	39	theme	growth	1408:1413	arg1	VEGF					1423:1426	VEGF	1423:1426	VEGF	1423:1426	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	39	theme	growth	1408:1413	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	39	theme	growth	1408:1413	arg1	factor					1415:1420	vascular endothelial growth factor	1387:1420	vascular endothelial growth factor (VEGF)	1387:1427	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	3	40	theme	plain	467:471	arg1	gauze					473:477	plain gauze	467:477	plain gauze	467:477	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	13	41	theme	accessible	2190:2199	arg1	materials					2201:2209	widely accessible materials	2183:2209	widely accessible materials	2183:2209	In this study, we prepared multifunctional composite dressings by a facile preparation process using widely accessible materials.					
30599244	0	42	theme	wound	69:73	arg1	healing					75:81	bacteria-infected wound healing	51:81	bacteria-infected wound healing	51:81	Cobalt-mediated multi-functional dressings promote bacteria-infected wound healing.					
30599244	1	43	theme	multiple	105:112	arg1	functions					114:122	multiple functions	105:122	multiple functions	105:122	Wound dressings with multiple functions are required to meet the complexity of the wound healing process.					
30599244	14	44	theme	hypoxia-like	2421:2432	arg1	induction					2434:2442	the hypoxia-like induction	2417:2442	the hypoxia-like induction	2417:2442	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	0	45	theme	Cobalt-mediated	0:14	arg1	dressings					33:41	Cobalt-mediated multi-functional dressings	0:41	Cobalt-mediated multi-functional dressings	0:41	Cobalt-mediated multi-functional dressings promote bacteria-infected wound healing.					
30599244	7	46	theme	hemostatic	1175:1184	arg1	property					1186:1193	hemostatic property	1175:1193	hemostatic property	1175:1193	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	6	47	theme	Co2+	879:882	arg1	properties					814:823	strong mechanical properties	796:823	strong mechanical properties	796:823	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	47	theme	Co2+	879:882	arg1	dispersion					834:843	uniform dispersion	826:843	uniform dispersion	826:843	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	47	theme	Co2+	879:882	arg1	release					859:865	sustained release	849:865	sustained release of Ca2+ and Co2+	849:882	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	4	48	theme	different	561:569	arg1	concentration					571:583	different concentration	561:583	different concentration	561:583	Firstly, mixed Ca2+/Co2+ ion solutions with different concentration were applied to gauzes.					
30599244	8	49	theme	transforming	1430:1441	arg1	expression					1477:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	49	theme	transforming	1430:1441	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	10	50	theme	Co2+0.25-Ca2+4 GSA	1749:1766	arg1	dressings					1778:1786	Co2+0.25-Ca2+4 GSA composite dressings	1749:1786	Co2+0.25-Ca2+4 GSA composite dressings	1749:1786	Collectively, our results indicated that Co2+0.25-Ca2+4 GSA composite dressings promote wound healing.					
30599244	0	51	theme	bacteria-infected	51:67	arg1	healing					75:81	bacteria-infected wound healing	51:81	bacteria-infected wound healing	51:81	Cobalt-mediated multi-functional dressings promote bacteria-infected wound healing.					
30599244	12	52	theme	preparation	1991:2001	arg1	complexity					2003:2012	preparation complexity	1991:2012	preparation complexity	1991:2012	However, there is often a trade-off between reducing preparation complexity and increasing the multifunctionality of the dressing's properties.					
30599244	6	53	theme	Ca2+	870:873	arg1	properties					814:823	strong mechanical properties	796:823	strong mechanical properties	796:823	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	53	theme	Ca2+	870:873	arg1	dispersion					834:843	uniform dispersion	826:843	uniform dispersion	826:843	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	53	theme	Ca2+	870:873	arg1	release					859:865	sustained release	849:865	sustained release of Ca2+ and Co2+	849:882	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	8	54	dep	In	1223:1224	arg1	vivo					1226:1229	vivo	1226:1229	vivo	1226:1229	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	55	theme	factor-β1	1450:1458	arg1	expression					1477:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	55	theme	factor-β1	1450:1458	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	13	56	theme	facile	2150:2155	arg1	preparation					2157:2167	a facile preparation	2148:2167	a facile preparation process using widely accessible materials	2148:2209	In this study, we prepared multifunctional composite dressings by a facile preparation process using widely accessible materials.					
30599244	16	57	theme	good	2686:2689	arg1	effect					2703:2708	good therapeutic effect	2686:2708	good therapeutic effect	2686:2708	Thus, we believed that the composite dressings can be widely applied in skin wound repair duo to their facile preparation method and good therapeutic effect.					
30599244	9	58	theme	dense	1681:1685	arg1	deposition					1696:1705	dense collagen deposition	1681:1705	dense collagen deposition	1681:1705	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	9	58	theme	dense	1681:1685	arg1	macrophages					1632:1642	macrophages	1632:1642	macrophages regulation, re-epithelialization and dense collagen deposition	1632:1705	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	4	59	theme	Ca2+/Co2+	532:540	arg1	solutions					546:554	mixed Ca2+/Co2+ ion solutions	526:554	mixed Ca2+/Co2+ ion solutions with different concentration	526:583	Firstly, mixed Ca2+/Co2+ ion solutions with different concentration were applied to gauzes.					
30599244	8	60	theme	TGF-β1	1461:1466	arg1	expression					1477:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	60	theme	TGF-β1	1461:1466	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	10	61	theme	wound	1796:1800	arg1	healing					1802:1808	wound healing	1796:1808	wound healing	1796:1808	Collectively, our results indicated that Co2+0.25-Ca2+4 GSA composite dressings promote wound healing.					
30599244	14	62	theme	hydrogels	2398:2406	arg1	capacity					2369:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity	2284:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels	2284:2406	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	8	63	theme	protein	1469:1475	arg1	expression					1477:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	63	theme	protein	1469:1475	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	6	64	theme	In	735:736	arg1	results					744:750	In vitro results	735:750	In vitro results	735:750	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	3	65	dep	surmount	301:308	arg1	gauze					473:477	plain gauze	467:477	plain gauze	467:477	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	3	65	dep	surmount	301:308	arg1	Co2+					511:514	Co2+	511:514	Co2+	511:514	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	3	65	dep	surmount	301:308	arg1	SA					497:498	SA	497:498	SA	497:498	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	3	65	dep	surmount	301:308	arg1	Ca2+					502:505	Ca2+	502:505	Ca2+	502:505	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	3	65	dep	surmount	301:308	arg1	alginate					487:494	sodium alginate	480:494	sodium alginate (SA)	480:499	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	7	66	theme	hypoxia-like	1119:1130	arg1	response					1132:1139	a hypoxia-like response	1117:1139	a hypoxia-like response	1117:1139	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	16	67	theme	skin	2625:2628	arg1	repair					2636:2641	skin wound repair duo	2625:2645	skin wound repair duo	2625:2645	Thus, we believed that the composite dressings can be widely applied in skin wound repair duo to their facile preparation method and good therapeutic effect.					
30599244	14	68	theme	hemostatic	2349:2358	arg1	property					2360:2367	hemostatic property	2349:2367	hemostatic property	2349:2367	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	5	69	theme	SA	623:624	arg1	solution					626:633	SA solution	623:633	SA solution	623:633	After drying, SA solution was added to ionized gauze and Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings were formed easily.					
30599244	6	70	theme	mechanical	803:812	arg1	properties					814:823	strong mechanical properties	796:823	strong mechanical properties	796:823	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	70	theme	mechanical	803:812	arg1	dispersion					834:843	uniform dispersion	826:843	uniform dispersion	826:843	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	70	theme	mechanical	803:812	arg1	release					859:865	sustained release	849:865	sustained release of Ca2+ and Co2+	849:882	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	14	71	theme	maintenance	2333:2343	arg1	capacity					2369:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity	2284:2376	the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels	2284:2406	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	13	72	theme	composite	2125:2133	arg1	dressings					2135:2143	multifunctional composite dressings	2109:2143	multifunctional composite dressings	2109:2143	In this study, we prepared multifunctional composite dressings by a facile preparation process using widely accessible materials.					
30599244	8	73	theme	Co2+0.25-Ca2+4 GSA	1266:1283	arg1	dressings					1295:1303	Co2+0.25-Ca2+4 GSA composite dressings	1266:1303	Co2+0.25-Ca2+4 GSA composite dressings	1266:1303	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	5	74	theme	composite	695:703	arg1	dressings					705:713	Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings	666:713	Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings	666:713	After drying, SA solution was added to ionized gauze and Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings were formed easily.					
30599244	6	75	theme	Ion-GSA	768:774	arg1	dressings					776:784	all Ion-GSA dressings	764:784	all Ion-GSA dressings	764:784	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	9	76	theme	quick	1584:1588	arg1	wounds					1598:1603	The quick healing wounds	1580:1603	The quick healing wounds	1580:1603	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	14	77	theme	exudate	2304:2310	arg1	absorption					2312:2321	the effective wound exudate absorption	2284:2321	the effective wound exudate absorption	2284:2321	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	11	78	theme	complex	1907:1913	arg1	requirements					1924:1935	complex clinical requirements	1907:1935	complex clinical requirements	1907:1935	STATEMENT OF SIGNIFICANCE: Wound dressings with integrated functionalities are required to meet complex clinical requirements.					
30599244	12	79	theme	properties	2070:2079	arg1	multifunctionality					2033:2050	the multifunctionality	2029:2050	the multifunctionality of the dressing's properties	2029:2079	However, there is often a trade-off between reducing preparation complexity and increasing the multifunctionality of the dressing's properties.					
30599244	8	80	theme	bacterial	1315:1323	arg1	growth					1325:1330	bacterial growth	1315:1330	bacterial growth	1315:1330	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	14	81	theme	effective	2288:2296	arg1	absorption					2312:2321	the effective wound exudate absorption	2284:2321	the effective wound exudate absorption	2284:2321	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	7	82	theme	Co2+0.25-Ca2+4 GSA	1030:1047	arg1	Ca2+					1024:1027	4 g/L Ca2+	1018:1027	4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings)	1018:1068	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	7	82	theme	Co2+0.25-Ca2+4 GSA	1030:1047	arg1	dressings					1059:1067	Co2+0.25-Ca2+4 GSA composite dressings	1030:1067	Co2+0.25-Ca2+4 GSA composite dressings	1030:1067	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	7	83	theme	4 g/L	1018:1022	arg1	Ca2+					1024:1027	4 g/L Ca2+	1018:1027	4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings)	1018:1068	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	7	83	theme	4 g/L	1018:1022	arg1	dressings					1059:1067	Co2+0.25-Ca2+4 GSA composite dressings	1030:1067	Co2+0.25-Ca2+4 GSA composite dressings	1030:1067	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	8	84	theme	healing	1237:1243	arg1	assays					1245:1250	In vivo wound healing assays	1223:1250	In vivo wound healing assays	1223:1250	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	6	85	theme	wound	931:935	arg1	exudate					937:943	wound exudate	931:943	wound exudate	931:943	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	8	86	theme	skin	1520:1523	arg1	healing					1531:1537	full-thickness skin wound healing	1505:1537	full-thickness skin wound healing	1505:1537	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	5	87	theme	Ion-GSA	686:692	arg1	dressings					705:713	Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings	666:713	Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings	666:713	After drying, SA solution was added to ionized gauze and Co2+-Ca2+/Gauze/SA (Ion-GSA) composite dressings were formed easily.					
30599244	15	88	theme	wound	2538:2542	arg1	healing					2544:2550	bacteria-infected wound healing	2520:2550	bacteria-infected wound healing	2520:2550	These functions all together promote bacteria-infected wound healing.					
30599244	9	89	theme	healing	1590:1596	arg1	wounds					1598:1603	The quick healing wounds	1580:1603	The quick healing wounds	1580:1603	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	8	90	theme	local	1343:1347	arg1	expression					1477:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	90	theme	local	1343:1347	arg1	HIF-1α					1378:1383	HIF-1α	1378:1383	HIF-1α	1378:1383	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	90	theme	local	1343:1347	arg1	factor					1415:1420	vascular endothelial growth factor	1387:1420	vascular endothelial growth factor (VEGF)	1387:1427	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	90	theme	local	1343:1347	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	11	91	theme	integrated	1859:1868	arg1	functionalities					1870:1884	integrated functionalities	1859:1884	integrated functionalities	1859:1884	STATEMENT OF SIGNIFICANCE: Wound dressings with integrated functionalities are required to meet complex clinical requirements.					
30599244	11	92	with	dressings	1844:1852	arg1	functionalities					1870:1884	integrated functionalities	1859:1884	integrated functionalities	1859:1884	STATEMENT OF SIGNIFICANCE: Wound dressings with integrated functionalities are required to meet complex clinical requirements.					
30599244	8	93	theme	mouse	1542:1546	arg1	model					1573:1577	mouse bacterial-infected wound model	1542:1577	mouse bacterial-infected wound model	1542:1577	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	14	94	theme	Co2+	2477:2480	arg1	induction					2434:2442	the hypoxia-like induction	2417:2442	the hypoxia-like induction	2417:2442	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	14	94	theme	Co2+	2477:2480	arg1	effects					2466:2472	the antimicrobial effects	2448:2472	the antimicrobial effects of Co2+	2448:2480	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	11	95	theme	dressings	1844:1852	arg1	STATEMENT					1811:1819	STATEMENT	1811:1819	STATEMENT OF SIGNIFICANCE: Wound dressings with integrated functionalities	1811:1884	STATEMENT OF SIGNIFICANCE: Wound dressings with integrated functionalities are required to meet complex clinical requirements.					
30599244	1	96	theme	healing	173:179	arg1	process					181:187	the wound healing process	163:187	the wound healing process	163:187	Wound dressings with multiple functions are required to meet the complexity of the wound healing process.					
30599244	3	97	theme	facile	334:339	arg1	preparation					341:351	a facile preparation	332:351	a facile preparation	332:351	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	8	98	theme	wound	1567:1571	arg1	model					1573:1577	mouse bacterial-infected wound model	1542:1577	mouse bacterial-infected wound model	1542:1577	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	1	99	theme	Wound	84:88	arg1	dressings					90:98	Wound dressings	84:98	Wound dressings with multiple functions	84:122	Wound dressings with multiple functions are required to meet the complexity of the wound healing process.					
30599244	7	100	theme	antibacterial	1199:1211	arg1	activity					1213:1220	antibacterial activity	1199:1220	antibacterial activity	1199:1220	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	14	101	theme	mechanical	2250:2259	arg1	strength					2261:2268	the mechanical strength	2246:2268	the mechanical strength of gauze	2246:2277	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	14	102	contain	possessed	2236:2244	arg2	strength					2261:2268	the mechanical strength	2246:2268	the mechanical strength of gauze	2246:2277	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	14	102	contain	possessed	2236:2244	arg1	dressings					2226:2234	The composite dressings	2212:2234	The composite dressings	2212:2234	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	8	103	theme	endothelial	1396:1406	arg1	VEGF					1423:1426	VEGF	1423:1426	VEGF	1423:1426	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	103	theme	endothelial	1396:1406	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	103	theme	endothelial	1396:1406	arg1	factor					1415:1420	vascular endothelial growth factor	1387:1420	vascular endothelial growth factor (VEGF)	1387:1427	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	14	104	contain	had	2413:2415	arg2	induction					2434:2442	the hypoxia-like induction	2417:2442	the hypoxia-like induction	2417:2442	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	14	104	contain	had	2413:2415	arg2	effects					2466:2472	the antimicrobial effects	2448:2472	the antimicrobial effects of Co2+	2448:2480	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	14	104	contain	had	2413:2415	arg1	dressings					2226:2234	The composite dressings	2212:2234	The composite dressings	2212:2234	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	0	105	theme	multi-functional	16:31	arg1	dressings					33:41	Cobalt-mediated multi-functional dressings	0:41	Cobalt-mediated multi-functional dressings	0:41	Cobalt-mediated multi-functional dressings promote bacteria-infected wound healing.					
30599244	2	106	from	complexity	247:256	arg1	preparation					285:295	dressing preparation	276:295	dressing preparation	276:295	The multifunctionality often leads to an increase in the complexity and difficulty in dressing preparation.					
30599244	2	107	from	difficulty	262:271	arg1	preparation					285:295	dressing preparation	276:295	dressing preparation	276:295	The multifunctionality often leads to an increase in the complexity and difficulty in dressing preparation.					
30599244	3	108	theme	multi-functional	392:407	arg1	dressing					409:416	a multi-functional dressing	390:416	a multi-functional dressing	390:416	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	14	109	theme	composite	2216:2224	arg1	dressings					2226:2234	The composite dressings	2212:2234	The composite dressings	2212:2234	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
30599244	10	110	theme	composite	1768:1776	arg1	dressings					1778:1786	Co2+0.25-Ca2+4 GSA composite dressings	1749:1786	Co2+0.25-Ca2+4 GSA composite dressings	1749:1786	Collectively, our results indicated that Co2+0.25-Ca2+4 GSA composite dressings promote wound healing.					
30599244	7	111	theme	favorable	1146:1154	arg1	cytocompatibility					1156:1172	favorable cytocompatibility	1146:1172	favorable cytocompatibility	1146:1172	Besides the above advantages, dressings prepared with 0.25 g/L Co2+ and 4 g/L Ca2+ (Co2+0.25-Ca2+4 GSA composite dressings) exhibited the best overall effect for inducing a hypoxia-like response, and favorable cytocompatibility, hemostatic property and antibacterial activity.					
30599244	3	112	theme	accessible	445:454	arg1	materials					456:464	four widely accessible materials	433:464	four widely accessible materials	433:464	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	4	113	theme	mixed	526:530	arg1	solutions					546:554	mixed Ca2+/Co2+ ion solutions	526:554	mixed Ca2+/Co2+ ion solutions with different concentration	526:583	Firstly, mixed Ca2+/Co2+ ion solutions with different concentration were applied to gauzes.					
30599244	3	114	theme	sodium	480:485	arg1	SA					497:498	SA	497:498	SA	497:498	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	3	114	theme	sodium	480:485	arg1	alginate					487:494	sodium alginate	480:494	sodium alginate (SA)	480:499	To surmount this problem, we used a facile preparation and fabrication process to fabricate a multi-functional dressing by integrating four widely accessible materials: plain gauze, sodium alginate (SA), Ca2+ and Co2+.					
30599244	16	115	theme	preparation	2663:2673	arg1	method					2675:2680	their facile preparation method	2650:2680	their facile preparation method	2650:2680	Thus, we believed that the composite dressings can be widely applied in skin wound repair duo to their facile preparation method and good therapeutic effect.					
30599244	4	116	theme	ion	542:544	arg1	solutions					546:554	mixed Ca2+/Co2+ ion solutions	526:554	mixed Ca2+/Co2+ ion solutions with different concentration	526:583	Firstly, mixed Ca2+/Co2+ ion solutions with different concentration were applied to gauzes.					
30599244	11	117	theme	SIGNIFICANCE	1824:1835	arg1	dressings					1844:1852	SIGNIFICANCE: Wound dressings	1824:1852	SIGNIFICANCE: Wound dressings with integrated functionalities	1824:1884	STATEMENT OF SIGNIFICANCE: Wound dressings with integrated functionalities are required to meet complex clinical requirements.					
30599244	8	118	theme	growth	1443:1448	arg1	expression					1477:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	transforming growth factor-β1 (TGF-β1) protein expression	1430:1486	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	8	118	theme	growth	1443:1448	arg1	factor-1α					1367:1375	local Hypoxia-inducible factor-1α	1343:1375	local Hypoxia-inducible factor-1α (HIF-1α)	1343:1384	In vivo wound healing assays revealed that Co2+0.25-Ca2+4 GSA composite dressings inhibited bacterial growth, increased local Hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) protein expression, and accelerated full-thickness skin wound healing in mouse bacterial-infected wound model.					
30599244	13	119	theme	preparation	2157:2167	arg1	process					2169:2175	a facile preparation process	2148:2175	a facile preparation process using widely accessible materials	2148:2209	In this study, we prepared multifunctional composite dressings by a facile preparation process using widely accessible materials.					
30599244	6	120	theme	sustained	849:857	arg1	properties					814:823	strong mechanical properties	796:823	strong mechanical properties	796:823	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	6	120	theme	sustained	849:857	arg1	release					859:865	sustained release	849:865	sustained release of Ca2+ and Co2+	849:882	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	9	121	theme	collagen	1687:1694	arg1	deposition					1696:1705	dense collagen deposition	1681:1705	dense collagen deposition	1681:1705	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	9	121	theme	collagen	1687:1694	arg1	macrophages					1632:1642	macrophages	1632:1642	macrophages regulation, re-epithelialization and dense collagen deposition	1632:1705	The quick healing wounds had improved angiogenesis, macrophages regulation, re-epithelialization and dense collagen deposition.					
30599244	13	122	theme	multifunctional	2109:2123	arg1	dressings					2135:2143	multifunctional composite dressings	2109:2143	multifunctional composite dressings	2109:2143	In this study, we prepared multifunctional composite dressings by a facile preparation process using widely accessible materials.					
30599244	16	123	theme	therapeutic	2691:2701	arg1	effect					2703:2708	good therapeutic effect	2686:2708	good therapeutic effect	2686:2708	Thus, we believed that the composite dressings can be widely applied in skin wound repair duo to their facile preparation method and good therapeutic effect.					
30599244	6	124	dep	In	735:736	arg1	vitro					738:742	vitro	738:742	vitro	738:742	In vitro results showed that all Ion-GSA dressings exhibited strong mechanical properties, uniform dispersion and sustained release of Ca2+ and Co2+, and the ability to retain moisture and absorb wound exudate.					
30599244	14	125	theme	alginate	2389:2396	arg1	hydrogels					2398:2406	calcium alginate hydrogels	2381:2406	calcium alginate hydrogels	2381:2406	The composite dressings possessed the mechanical strength of gauze, had the effective wound exudate absorption, moisture maintenance and hemostatic property capacity of calcium alginate hydrogels, and had the hypoxia-like induction and the antimicrobial effects of Co2+.					
31336152	7	0	theme	significant	1043:1053	arg1	differences					1070:1080	significant microstructure differences	1043:1080	significant microstructure differences between hydrophobic-rich and hydrophilic-rich TPU membranes	1043:1140	Imaging revealed significant microstructure differences between hydrophobic-rich and hydrophilic-rich TPU membranes, supporting hypotheses proposed in our previous study.					
31336152	4	1	theme	composition	571:581	arg1	membranes					538:546	membranes	538:546	membranes of varying TPU mixture composition	538:581	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	4	2	theme	physicochemical	487:501	arg1	properties					503:512	drug physicochemical properties	482:512	drug physicochemical properties	482:512	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	9	3	with	drugs	1576:1580	arg1	properties					1611:1620	varying physicochemical properties	1587:1620	varying physicochemical properties	1587:1620	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	1	4	theme	patient	130:136	arg1	adherence					138:146	patient adherence	130:146	patient adherence	130:146	Implants offer the opportunity to improve patient adherence and real-world outcomes.					
31336152	4	5	theme	mixture	563:569	arg1	composition					571:581	varying TPU mixture composition	551:581	varying TPU mixture composition	551:581	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	4	6	theme	drug	482:485	arg1	properties					503:512	drug physicochemical properties	482:512	drug physicochemical properties	482:512	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	8	7	theme	molecular	1413:1421	arg1	weight					1423:1428	small molecular weight	1407:1428	small molecular weight drugs	1407:1434	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	4	8	theme	leverage	584:591	arg1	imaging					593:599	leverage imaging	584:599	leverage imaging	584:599	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	6	9	theme	hydrophilic-rich	946:961	arg1	membranes					967:975	hydrophilic-rich TPU membranes	946:975	hydrophilic-rich TPU membranes	946:975	Conversely, all compounds diffused through hydrophilic-rich TPU membranes at similar rates, regardless of drug properties.					
31336152	4	10	from	effect	472:477	arg1	permeability					517:528	permeability	517:528	permeability through membranes of varying TPU mixture composition	517:581	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	8	11	theme	small	1407:1411	arg1	weight					1423:1428	small molecular weight	1407:1428	small molecular weight drugs	1407:1434	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	0	12	theme	properties	76:85	arg1	drugs					60:64	drugs	60:64	drugs of varied properties	60:85	How broadly can poly(urethane)-based implants be applied to drugs of varied properties?					
31336152	9	13	theme	release	1560:1566	arg1	rate					1568:1571	the release rate	1556:1571	the release rate of drugs with varying physicochemical properties	1556:1620	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	2	14	theme	suitable	249:256	arg1	properties					238:247	drug properties	233:247	drug properties suitable for development	233:272	However, most polymers used today are hydrophobic and limit drug properties suitable for development.					
31336152	1	15	theme	real-world	152:161	arg1	outcomes					163:170	real-world outcomes	152:170	real-world outcomes	152:170	Implants offer the opportunity to improve patient adherence and real-world outcomes.					
31336152	9	16	theme	rate-controlling	1517:1532	arg1	benefits					1485:1492	the benefits	1481:1492	the benefits of hydrophilic TPUs	1481:1512	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	9	16	theme	rate-controlling	1517:1532	arg1	membranes					1534:1542	rate-controlling membranes	1517:1542	rate-controlling membranes	1517:1542	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	0	17	theme	varied	69:74	arg1	properties					76:85	varied properties	69:85	varied properties	69:85	How broadly can poly(urethane)-based implants be applied to drugs of varied properties?					
31336152	8	18	theme	weight	1423:1428	arg1	drugs					1430:1434	small molecular weight drugs	1407:1434	small molecular weight drugs	1407:1434	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	6	19	theme	drug	1009:1012	arg1	properties					1014:1023	drug properties	1009:1023	drug properties	1009:1023	Conversely, all compounds diffused through hydrophilic-rich TPU membranes at similar rates, regardless of drug properties.					
31336152	7	20	theme	previous	1181:1188	arg1	study					1190:1194	our previous study	1177:1194	our previous study	1177:1194	Imaging revealed significant microstructure differences between hydrophobic-rich and hydrophilic-rich TPU membranes, supporting hypotheses proposed in our previous study.					
31336152	8	21	theme	macromolecules	1440:1453	arg1	release					1396:1402	the release	1392:1402	the release of small molecular weight drugs and macromolecules	1392:1453	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	4	22	theme	mixture	669:675	arg1	range					689:693	the TPU mixture composition range	661:693	the TPU mixture composition range	661:693	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	9	23	theme	TPUs	1509:1512	arg1	benefits					1485:1492	the benefits	1481:1492	the benefits of hydrophilic TPUs	1481:1512	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	9	23	theme	TPUs	1509:1512	arg1	membranes					1534:1542	rate-controlling membranes	1517:1542	rate-controlling membranes	1517:1542	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	4	24	theme	TPU	665:667	arg1	range					689:693	the TPU mixture composition range	661:693	the TPU mixture composition range	661:693	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	8	25	theme	pore	1292:1295	arg1	radius					1297:1302	its equivalent pore radius	1277:1302	its equivalent pore radius	1277:1302	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	4	26	theme	pore	773:776	arg1	analysis					778:785	equivalent pore analysis	762:785	equivalent pore analysis	762:785	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	6	27	theme	TPU	963:965	arg1	membranes					967:975	hydrophilic-rich TPU membranes	946:975	hydrophilic-rich TPU membranes	946:975	Conversely, all compounds diffused through hydrophilic-rich TPU membranes at similar rates, regardless of drug properties.					
31336152	8	28	theme	equivalent	1281:1290	arg1	radius					1297:1302	its equivalent pore radius	1277:1302	its equivalent pore radius	1277:1302	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	4	29	theme	TPU	559:561	arg1	composition					571:581	varying TPU mixture composition	551:581	varying TPU mixture composition	551:581	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	8	30	theme	drugs	1430:1434	arg1	release					1396:1402	the release	1392:1402	the release of small molecular weight drugs and macromolecules	1392:1453	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	4	31	theme	equivalent	762:771	arg1	analysis					778:785	equivalent pore analysis	762:785	equivalent pore analysis	762:785	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	5	32	theme	hydrophobic-rich	871:886	arg1	membranes					892:900	hydrophobic-rich TPU membranes	871:900	hydrophobic-rich TPU membranes	871:900	We observed a correlation between drug hydrophobicity and its permeability through hydrophobic-rich TPU membranes.					
31336152	4	33	theme	varying	551:557	arg1	composition					571:581	varying TPU mixture composition	551:581	varying TPU mixture composition	551:581	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	5	34	theme	TPU	888:890	arg1	membranes					892:900	hydrophobic-rich TPU membranes	871:900	hydrophobic-rich TPU membranes	871:900	We observed a correlation between drug hydrophobicity and its permeability through hydrophobic-rich TPU membranes.					
31336152	8	35	used	used	1377:1380	arg2	membranes					1360:1368	hydrophilic TPU membranes	1344:1368	hydrophilic TPU membranes	1344:1368	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	3	36	theme	different	421:429	arg1	properties					431:440	broadly different properties	413:440	broadly different properties	413:440	Thermoplastic poly(urethanes) (TPUs) form pores upon hydration and may facilitate the development of implants containing drugs exhibiting broadly different properties.					
31336152	3	37	theme	implants	376:383	arg1	development					361:371	the development	357:371	the development of implants containing drugs exhibiting broadly different properties	357:440	Thermoplastic poly(urethanes) (TPUs) form pores upon hydration and may facilitate the development of implants containing drugs exhibiting broadly different properties.					
31336152	9	38	theme	hydrophilic	1497:1507	arg1	TPUs					1509:1512	hydrophilic TPUs	1497:1512	hydrophilic TPUs	1497:1512	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	8	39	theme	TPU	1356:1358	arg1	membranes					1360:1368	hydrophilic TPU membranes	1344:1368	hydrophilic TPU membranes	1344:1368	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	0	40	theme	-based	30:35	arg1	implants					37:44	poly(urethane)-based implants	16:44	poly(urethane)-based implants	16:44	How broadly can poly(urethane)-based implants be applied to drugs of varied properties?					
31336152	4	41	theme	properties	503:512	arg1	effect					472:477	the effect	468:477	the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition	468:581	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	8	42	theme	hydrophilic	1210:1220	arg1	area					1240:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	8	42	theme	hydrophilic	1210:1220	arg1	%					1271:1271	0.583%	1266:1271	0.583%	1266:1271	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	7	43	theme	hydrophilic-rich	1111:1126	arg1	membranes					1132:1140	hydrophobic-rich and hydrophilic-rich TPU membranes	1090:1140	hydrophobic-rich and hydrophilic-rich TPU membranes	1090:1140	Imaging revealed significant microstructure differences between hydrophobic-rich and hydrophilic-rich TPU membranes, supporting hypotheses proposed in our previous study.					
31336152	9	44	theme	drugs	1576:1580	arg1	rate					1568:1571	the release rate	1556:1571	the release rate of drugs with varying physicochemical properties	1556:1620	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	5	45	theme	drug	822:825	arg1	hydrophobicity					827:840	drug hydrophobicity	822:840	drug hydrophobicity	822:840	We observed a correlation between drug hydrophobicity and its permeability through hydrophobic-rich TPU membranes.					
31336152	8	46	theme	hydrated	1201:1208	arg1	area					1240:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	8	46	theme	hydrated	1201:1208	arg1	%					1271:1271	0.583%	1266:1271	0.583%	1266:1271	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	2	47	theme	most	182:185	arg1	polymers					187:194	most polymers	182:194	most polymers used today	182:205	However, most polymers used today are hydrophobic and limit drug properties suitable for development.					
31336152	7	48	theme	TPU	1128:1130	arg1	membranes					1132:1140	hydrophobic-rich and hydrophilic-rich TPU membranes	1090:1140	hydrophobic-rich and hydrophilic-rich TPU membranes	1090:1140	Imaging revealed significant microstructure differences between hydrophobic-rich and hydrophilic-rich TPU membranes, supporting hypotheses proposed in our previous study.					
31336152	3	49	contain	containing	385:394	arg2	drugs					396:400	drugs	396:400	drugs exhibiting broadly different properties	396:440	Thermoplastic poly(urethanes) (TPUs) form pores upon hydration and may facilitate the development of implants containing drugs exhibiting broadly different properties.					
31336152	3	49	contain	containing	385:394	arg1	implants					376:383	implants	376:383	implants containing drugs exhibiting broadly different properties	376:440	Thermoplastic poly(urethanes) (TPUs) form pores upon hydration and may facilitate the development of implants containing drugs exhibiting broadly different properties.					
31336152	2	50	theme	drug	233:236	arg1	properties					238:247	drug properties	233:247	drug properties suitable for development	233:272	However, most polymers used today are hydrophobic and limit drug properties suitable for development.					
31336152	7	51	theme	hydrophobic-rich	1090:1105	arg1	membranes					1132:1140	hydrophobic-rich and hydrophilic-rich TPU membranes	1090:1140	hydrophobic-rich and hydrophilic-rich TPU membranes	1090:1140	Imaging revealed significant microstructure differences between hydrophobic-rich and hydrophilic-rich TPU membranes, supporting hypotheses proposed in our previous study.					
31336152	4	52	theme	membrane	732:739	arg1	microstructure					741:754	the membrane microstructure	728:754	the membrane microstructure	728:754	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	3	53	theme	Thermoplastic	275:287	arg1	urethanes					294:302	urethanes	294:302	urethanes	294:302	Thermoplastic poly(urethanes) (TPUs) form pores upon hydration and may facilitate the development of implants containing drugs exhibiting broadly different properties.					
31336152	3	53	theme	Thermoplastic	275:287	arg1	TPUs					306:309	TPUs	306:309	TPUs	306:309	Thermoplastic poly(urethanes) (TPUs) form pores upon hydration and may facilitate the development of implants containing drugs exhibiting broadly different properties.					
31336152	3	53	theme	Thermoplastic	275:287	arg1	poly					289:292	Thermoplastic poly	275:292	Thermoplastic poly(urethanes) (TPUs)	275:310	Thermoplastic poly(urethanes) (TPUs) form pores upon hydration and may facilitate the development of implants containing drugs exhibiting broadly different properties.					
31336152	7	54	theme	microstructure	1055:1068	arg1	differences					1070:1080	significant microstructure differences	1043:1080	significant microstructure differences between hydrophobic-rich and hydrophilic-rich TPU membranes	1043:1140	Imaging revealed significant microstructure differences between hydrophobic-rich and hydrophilic-rich TPU membranes, supporting hypotheses proposed in our previous study.					
31336152	9	55	theme	physicochemical	1595:1609	arg1	properties					1611:1620	varying physicochemical properties	1587:1620	varying physicochemical properties	1587:1620	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	9	56	theme	varying	1587:1593	arg1	properties					1611:1620	varying physicochemical properties	1587:1620	varying physicochemical properties	1587:1620	These findings highlight the benefits of hydrophilic TPUs as rate-controlling membranes to modulate the release rate of drugs with varying physicochemical properties.					
31336152	8	57	theme	membrane	1226:1233	arg1	area					1240:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	8	57	theme	membrane	1226:1233	arg1	%					1271:1271	0.583%	1266:1271	0.583%	1266:1271	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	8	58	theme	hydrophilic	1344:1354	arg1	membranes					1360:1368	hydrophilic TPU membranes	1344:1368	hydrophilic TPU membranes	1344:1368	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	4	59	theme	microstructural	614:628	arg1	changes					630:636	microstructural changes	614:636	microstructural changes to the membrane	614:652	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	8	60	theme	pore	1235:1238	arg1	area					1240:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	8	60	theme	pore	1235:1238	arg1	%					1271:1271	0.583%	1266:1271	0.583%	1266:1271	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	6	61	theme	similar	980:986	arg1	rates					988:992	similar rates	980:992	similar rates	980:992	Conversely, all compounds diffused through hydrophilic-rich TPU membranes at similar rates, regardless of drug properties.					
31336152	4	62	theme	composition	677:687	arg1	range					689:693	the TPU mixture composition range	661:693	the TPU mixture composition range	661:693	We sought to investigate the effect of drug physicochemical properties on permeability through membranes of varying TPU mixture composition; leverage imaging to visualize microstructural changes to the membrane across the TPU mixture composition range; and quantitatively characterize the membrane microstructure using equivalent pore analysis.					
31336152	8	63	theme	TPU	1222:1224	arg1	area					1240:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area	1197:1243	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31336152	8	63	theme	TPU	1222:1224	arg1	%					1271:1271	0.583%	1266:1271	0.583%	1266:1271	The hydrated hydrophilic TPU membrane pore area was determined to be 0.583% and its equivalent pore radius was found to be 128 nm, suggesting that hydrophilic TPU membranes may be used to modify the release of small molecular weight drugs and macromolecules.					
31597222	6	0	theme	epithelial	928:937	arg1	lineage					939:945	the epithelial lineage	924:945	the epithelial lineage	924:945	MSCs differentiation to the epithelial lineage was confirmed by staining for epithelial and stem cell specific markers.					
31597222	3	1	theme	Calu-3	516:521	arg1	cells					523:527	Calu-3 cells	516:527	Calu-3 cells	516:527	Our data show differentiation of Calu-3 cells on composite films as evidenced by tight junction protein expression and barrier formation.					
31597222	9	2	theme	factor	1441:1446	arg1	cocktail					1448:1455	growth factor cocktail	1434:1455	growth factor cocktail	1434:1455	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	2	3	theme	growth	298:303	arg1	factor					305:310	an epithelial cell growth factor	279:310	an epithelial cell growth factor cocktail	279:319	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	6	4	theme	MSCs	900:903	arg1	differentiation					905:919	MSCs differentiation	900:919	MSCs differentiation to the epithelial lineage	900:945	MSCs differentiation to the epithelial lineage was confirmed by staining for epithelial and stem cell specific markers.					
31597222	8	5	theme	markers	1262:1268	arg1	expression					1229:1238	the expression	1225:1238	the expression of epithelial lineage markers	1225:1268	However, despite the expression of epithelial lineage markers, these cells did not form fully functional tight junctions as evidenced by low expression of junctional protein ZO1.					
31597222	2	6	theme	epithelial	282:291	arg1	factor					305:310	an epithelial cell growth factor	279:310	an epithelial cell growth factor cocktail	279:319	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	5	7	theme	MSCs	874:877	arg1	differentiation					849:863	differentiation	849:863	differentiation of human MSCs to epithelial cells	849:897	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	3	8	theme	protein	579:585	arg1	expression					587:596	tight junction protein expression	564:596	tight junction protein expression	564:596	Our data show differentiation of Calu-3 cells on composite films as evidenced by tight junction protein expression and barrier formation.					
31597222	5	9	theme	epithelial	882:891	arg1	cells					893:897	epithelial cells	882:897	epithelial cells	882:897	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	10	10	theme	using	1626:1630	arg1	MSCs					1632:1635	using MSCs	1626:1635	using MSCs in epithelial tissue engineering	1626:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	3	11	theme	tight	564:568	arg1	expression					587:596	tight junction protein expression	564:596	tight junction protein expression	564:596	Our data show differentiation of Calu-3 cells on composite films as evidenced by tight junction protein expression and barrier formation.					
31597222	10	12	dep	in	1844:1845	arg1	vitro					1847:1851	vitro	1847:1851	vitro	1847:1851	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	5	13	theme	factor	820:825	arg1	platform					836:843	a biomimetic scaffold and growth factor delivery platform	787:843	a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells	787:897	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	1	14	theme	epithelial	232:241	arg1	patches					243:249	developing epithelial patches	221:249	developing epithelial patches	221:249	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	8	15	theme	epithelial	1243:1252	arg1	markers					1262:1268	epithelial lineage markers	1243:1268	epithelial lineage markers	1243:1268	However, despite the expression of epithelial lineage markers, these cells did not form fully functional tight junctions as evidenced by low expression of junctional protein ZO1.					
31597222	1	16	from	patches	243:249	arg1	fabrication					148:158	fabrication	148:158	fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches	148:249	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	8	17	theme	functional	1302:1311	arg1	junctions					1319:1327	fully functional tight junctions	1296:1327	fully functional tight junctions	1296:1327	However, despite the expression of epithelial lineage markers, these cells did not form fully functional tight junctions as evidenced by low expression of junctional protein ZO1.					
31597222	10	18	theme	tissue	1651:1656	arg1	engineering					1658:1668	epithelial tissue engineering	1640:1668	epithelial tissue engineering	1640:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	19	theme	barrier	1866:1872	arg1	tissues					1874:1880	barrier tissues	1866:1880	barrier tissues	1866:1880	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	1	20	from	application	206:216	arg1	patches					243:249	developing epithelial patches	221:249	developing epithelial patches	221:249	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	3	21	dep	films	542:546	arg1	evidenced					551:559	evidenced	551:559	evidenced by tight junction protein expression and barrier formation	551:618	Our data show differentiation of Calu-3 cells on composite films as evidenced by tight junction protein expression and barrier formation.					
31597222	4	22	theme	basal	699:703	arg1	A549					726:729	alveolar basal epithelial cell line A549	690:729	alveolar basal epithelial cell line A549	690:729	The films also supported attachment, migration, and proliferation of alveolar basal epithelial cell line A549.					
31597222	5	23	theme	delivery	827:834	arg1	platform					836:843	a biomimetic scaffold and growth factor delivery platform	787:843	a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells	787:897	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	4	24	theme	cell	716:719	arg1	A549					726:729	alveolar basal epithelial cell line A549	690:729	alveolar basal epithelial cell line A549	690:729	The films also supported attachment, migration, and proliferation of alveolar basal epithelial cell line A549.					
31597222	5	25	theme	films	778:782	arg1	suitability					749:759	the suitability	745:759	the suitability of the composite films	745:782	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	9	26	link	MSCs-derived	1508:1519	arg1	cells					1532:1536	MSCs-derived epithelial cells	1508:1536	MSCs-derived epithelial cells	1508:1536	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	1	27	theme	Gelatin-based	163:175	arg1	films					190:194	Gelatin-based biocomposite films	163:194	Gelatin-based biocomposite films	163:194	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	2	28	dep	in	367:368	arg1	vitro					370:374	vitro	370:374	vitro	370:374	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	0	29	theme	stem	88:91	arg1	patches					124:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell-derived epithelial tissue patches.					
31597222	6	30	theme	cell	997:1000	arg1	markers					1011:1017	epithelial and stem cell specific markers	977:1017	markers	1011:1017	MSCs differentiation to the epithelial lineage was confirmed by staining for epithelial and stem cell specific markers.					
31597222	10	31	theme	transferrable	1722:1734	arg1	matrix					1750:1755	transferrable extracellular matrix mimics	1722:1762	transferrable extracellular matrix mimics	1722:1762	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	8	32	theme	protein	1374:1380	arg1	ZO1					1382:1384	junctional protein ZO1	1363:1384	junctional protein ZO1	1363:1384	However, despite the expression of epithelial lineage markers, these cells did not form fully functional tight junctions as evidenced by low expression of junctional protein ZO1.					
31597222	2	33	theme	epithelium	414:423	arg1	regeneration					376:387	in vitro regeneration	367:387	in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs)	367:480	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	0	34	theme	epithelial	106:115	arg1	patches					124:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell-derived epithelial tissue patches.					
31597222	7	35	from	changes	1199:1205	arg1	vimentin					1131:1138	vimentin	1131:1138	vimentin	1131:1138	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	7	35	from	changes	1199:1205	arg1	expression					1169:1178	pan cytokeratin expression	1153:1178	pan cytokeratin expression	1153:1178	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	2	36	theme	organized	392:400	arg1	epithelium					414:423	organized respiratory epithelium	392:423	organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs)	392:480	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	5	37	theme	growth	813:818	arg1	factor					820:825	a biomimetic scaffold and growth factor	787:825	factor	820:825	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	2	38	theme	mesenchymal	452:462	arg1	MSCs					476:479	MSCs	476:479	MSCs	476:479	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	2	38	theme	mesenchymal	452:462	arg1	cells					469:473	mesenchymal stem cells	452:473	mesenchymal stem cells (MSCs)	452:480	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	10	39	theme	extracellular	1736:1748	arg1	matrix					1750:1755	transferrable extracellular matrix mimics	1722:1762	transferrable extracellular matrix mimics	1722:1762	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	0	40	theme	Gelatin/hyaluronic	12:29	arg1	hydrogel					36:43	A composite Gelatin/hyaluronic acid hydrogel	0:43	A composite Gelatin/hyaluronic acid hydrogel as an ECM	0:53	A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell-derived epithelial tissue patches.					
31597222	3	41	theme	composite	532:540	arg1	films					542:546	composite films	532:546	composite films as evidenced by tight junction protein expression and barrier formation	532:618	Our data show differentiation of Calu-3 cells on composite films as evidenced by tight junction protein expression and barrier formation.					
31597222	7	42	from	reduction	1118:1126	arg1	vimentin					1131:1138	vimentin	1131:1138	vimentin	1131:1138	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	7	42	from	reduction	1118:1126	arg1	expression					1169:1178	pan cytokeratin expression	1153:1178	pan cytokeratin expression	1153:1178	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	2	43	theme	Calu-3	431:436	arg1	line					443:446	Calu-3 cell line	431:446	Calu-3 cell line	431:446	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	1	44	theme	biocomposite	177:188	arg1	films					190:194	Gelatin-based biocomposite films	163:194	Gelatin-based biocomposite films	163:194	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	10	45	theme	delivery	1768:1775	arg1	applicability					1678:1690	the applicability	1674:1690	the applicability of the composite hydrogels	1674:1717	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	45	theme	delivery	1768:1775	arg1	possibility					1611:1621	the possibility	1607:1621	the possibility of using MSCs in epithelial tissue engineering	1607:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	45	theme	delivery	1768:1775	arg1	platforms					1777:1785	delivery platforms	1768:1785	delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues	1768:1880	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	5	46	theme	scaffold	800:807	arg1	platform					836:843	a biomimetic scaffold and growth factor delivery platform	787:843	a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells	787:897	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	9	47	theme	Further	1387:1393	arg1	optimisation					1395:1406	Further optimisation	1387:1406	Further optimisation of culture conditions and growth factor cocktail	1387:1455	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	7	48	from	increase	1141:1148	arg1	vimentin					1131:1138	vimentin	1131:1138	vimentin	1131:1138	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	7	48	from	increase	1141:1148	arg1	expression					1169:1178	pan cytokeratin expression	1153:1178	pan cytokeratin expression	1153:1178	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	2	49	theme	extracellular	336:348	arg1	matrix					350:355	an extracellular matrix	333:355	an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs)	333:480	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	2	49	theme	extracellular	336:348	arg1	films					256:260	The films	252:260	The films	252:260	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	2	50	used	used	325:328	arg2	films					256:260	The films	252:260	The films	252:260	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	2	50	used	used	325:328	arg2	matrix					350:355	an extracellular matrix	333:355	an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs)	333:480	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	3	51	theme	cells	523:527	arg1	differentiation					497:511	differentiation	497:511	differentiation of Calu-3 cells	497:527	Our data show differentiation of Calu-3 cells on composite films as evidenced by tight junction protein expression and barrier formation.					
31597222	1	52	theme	films	190:194	arg1	fabrication					148:158	fabrication	148:158	fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches	148:249	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	9	53	theme	conditions	1419:1428	arg1	optimisation					1395:1406	Further optimisation	1387:1406	Further optimisation of culture conditions and growth factor cocktail	1387:1455	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	10	54	dep	matrix	1750:1755	arg1	mimics					1757:1762	mimics	1757:1762	mimics	1757:1762	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	55	from	MSCs	1632:1635	arg1	engineering					1658:1668	epithelial tissue engineering	1640:1668	epithelial tissue engineering	1640:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	0	56	link	cell-derived	93:104	arg1	patches					124:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell-derived epithelial tissue patches.					
31597222	5	57	theme	composite	768:776	arg1	films					778:782	the composite films	764:782	the composite films	764:782	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	2	58	theme	in	367:368	arg1	regeneration					376:387	in vitro regeneration	367:387	in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs)	367:480	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	9	59	theme	growth	1434:1439	arg1	cocktail					1448:1455	growth factor cocktail	1434:1455	growth factor cocktail	1434:1455	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	2	60	theme	factor	305:310	arg1	cocktail					312:319	an epithelial cell growth factor cocktail	279:319	an epithelial cell growth factor cocktail	279:319	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	9	61	theme	cocktail	1448:1455	arg1	optimisation					1395:1406	Further optimisation	1387:1406	Further optimisation of culture conditions and growth factor cocktail	1387:1455	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	10	62	from	engineering	1658:1668	arg1	applicability					1678:1690	the applicability	1674:1690	the applicability of the composite hydrogels	1674:1717	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	62	from	engineering	1658:1668	arg1	possibility					1611:1621	the possibility	1607:1621	the possibility of using MSCs in epithelial tissue engineering	1607:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	62	from	engineering	1658:1668	arg1	platforms					1777:1785	delivery platforms	1768:1785	delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues	1768:1880	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	8	63	theme	low	1345:1347	arg1	expression					1349:1358	low expression	1345:1358	low expression of junctional protein ZO1	1345:1384	However, despite the expression of epithelial lineage markers, these cells did not form fully functional tight junctions as evidenced by low expression of junctional protein ZO1.					
31597222	7	64	theme	significant	1106:1116	arg1	reduction					1118:1126	significant reduction	1106:1126	significant reduction in vimentin	1106:1138	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	2	65	theme	cell	293:296	arg1	factor					305:310	an epithelial cell growth factor	279:310	an epithelial cell growth factor cocktail	279:319	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	5	66	theme	human	868:872	arg1	MSCs					874:877	human MSCs	868:877	human MSCs	868:877	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	7	67	theme	pan	1153:1155	arg1	expression					1169:1178	pan cytokeratin expression	1153:1178	pan cytokeratin expression	1153:1178	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	8	68	theme	lineage	1254:1260	arg1	markers					1262:1268	epithelial lineage markers	1243:1268	epithelial lineage markers	1243:1268	However, despite the expression of epithelial lineage markers, these cells did not form fully functional tight junctions as evidenced by low expression of junctional protein ZO1.					
31597222	10	69	theme	epithelial	1640:1649	arg1	engineering					1658:1668	epithelial tissue engineering	1640:1668	epithelial tissue engineering	1640:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	3	70	theme	junction	570:577	arg1	expression					587:596	tight junction protein expression	564:596	tight junction protein expression	564:596	Our data show differentiation of Calu-3 cells on composite films as evidenced by tight junction protein expression and barrier formation.					
31597222	10	71	theme	regenerative	1818:1829	arg1	medicine					1831:1838	regenerative medicine	1818:1838	regenerative medicine	1818:1838	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	6	72	theme	stem	992:995	arg1	markers					1011:1017	epithelial and stem cell specific markers	977:1017	markers	1011:1017	MSCs differentiation to the epithelial lineage was confirmed by staining for epithelial and stem cell specific markers.					
31597222	10	73	from	applications	1802:1813	arg1	medicine					1831:1838	regenerative medicine	1818:1838	regenerative medicine	1818:1838	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	74	theme	MSCs	1632:1635	arg1	applicability					1678:1690	the applicability	1674:1690	the applicability of the composite hydrogels	1674:1717	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	74	theme	MSCs	1632:1635	arg1	possibility					1611:1621	the possibility	1607:1621	the possibility of using MSCs in epithelial tissue engineering	1607:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	74	theme	MSCs	1632:1635	arg1	platforms					1777:1785	delivery platforms	1768:1785	delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues	1768:1880	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	1	75	theme	application	206:216	arg1	fabrication					148:158	fabrication	148:158	fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches	148:249	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	6	76	theme	epithelial	977:986	arg1	markers					1011:1017	epithelial and stem cell specific markers	977:1017	markers	1011:1017	MSCs differentiation to the epithelial lineage was confirmed by staining for epithelial and stem cell specific markers.					
31597222	7	77	theme	epithelial	1060:1069	arg1	characteristics					1071:1085	the epithelial characteristics	1056:1085	the epithelial characteristics	1056:1085	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	3	78	theme	barrier	602:608	arg1	formation					610:618	barrier formation	602:618	barrier formation	602:618	Our data show differentiation of Calu-3 cells on composite films as evidenced by tight junction protein expression and barrier formation.					
31597222	9	79	theme	tight	1480:1484	arg1	formation					1495:1503	tight junction formation	1480:1503	tight junction formation in MSCs-derived epithelial cells	1480:1536	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	1	80	theme	developing	221:230	arg1	patches					243:249	developing epithelial patches	221:249	developing epithelial patches	221:249	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	10	81	theme	tissues	1874:1880	arg1	modelling					1853:1861	in vitro modelling	1844:1861	in vitro modelling of barrier tissues	1844:1880	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	81	theme	tissues	1874:1880	arg1	applications					1802:1813	potential applications	1792:1813	potential applications in regenerative medicine	1792:1838	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	9	82	theme	epithelial	1521:1530	arg1	cells					1532:1536	MSCs-derived epithelial cells	1508:1536	MSCs-derived epithelial cells	1508:1536	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	4	83	theme	line	721:724	arg1	A549					726:729	alveolar basal epithelial cell line A549	690:729	alveolar basal epithelial cell line A549	690:729	The films also supported attachment, migration, and proliferation of alveolar basal epithelial cell line A549.					
31597222	4	84	theme	A549	726:729	arg1	migration					658:666	migration	658:666	migration	658:666	The films also supported attachment, migration, and proliferation of alveolar basal epithelial cell line A549.					
31597222	4	84	theme	A549	726:729	arg1	proliferation					673:685	proliferation	673:685	proliferation	673:685	The films also supported attachment, migration, and proliferation of alveolar basal epithelial cell line A549.					
31597222	4	84	theme	A549	726:729	arg1	attachment					646:655	attachment	646:655	attachment	646:655	The films also supported attachment, migration, and proliferation of alveolar basal epithelial cell line A549.					
31597222	8	85	theme	tight	1313:1317	arg1	junctions					1319:1327	fully functional tight junctions	1296:1327	fully functional tight junctions	1296:1327	However, despite the expression of epithelial lineage markers, these cells did not form fully functional tight junctions as evidenced by low expression of junctional protein ZO1.					
31597222	10	86	from	modelling	1853:1861	arg1	medicine					1831:1838	regenerative medicine	1818:1838	regenerative medicine	1818:1838	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	9	87	theme	junction	1486:1493	arg1	formation					1495:1503	tight junction formation	1480:1503	tight junction formation in MSCs-derived epithelial cells	1480:1536	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	9	88	theme	composite	1545:1553	arg1	hydrogels					1555:1563	the composite hydrogels	1541:1563	the composite hydrogels	1541:1563	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	4	89	theme	alveolar	690:697	arg1	A549					726:729	alveolar basal epithelial cell line A549	690:729	alveolar basal epithelial cell line A549	690:729	The films also supported attachment, migration, and proliferation of alveolar basal epithelial cell line A549.					
31597222	0	90	theme	mesenchymal	76:86	arg1	patches					124:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell-derived epithelial tissue patches.					
31597222	1	91	from	films	190:194	arg1	patches					243:249	developing epithelial patches	221:249	developing epithelial patches	221:249	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	4	92	theme	epithelial	705:714	arg1	A549					726:729	alveolar basal epithelial cell line A549	690:729	alveolar basal epithelial cell line A549	690:729	The films also supported attachment, migration, and proliferation of alveolar basal epithelial cell line A549.					
31597222	0	93	theme	cell-derived	93:104	arg1	patches					124:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell-derived epithelial tissue patches.					
31597222	10	94	theme	in	1844:1845	arg1	modelling					1853:1861	in vitro modelling	1844:1861	in vitro modelling of barrier tissues	1844:1880	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	6	95	theme	specific	1002:1009	arg1	markers					1011:1017	epithelial and stem cell specific markers	977:1017	markers	1011:1017	MSCs differentiation to the epithelial lineage was confirmed by staining for epithelial and stem cell specific markers.					
31597222	0	96	theme	tissue	117:122	arg1	patches					124:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	mesenchymal stem cell-derived epithelial tissue patches	76:130	A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell-derived epithelial tissue patches.					
31597222	1	97	from	fabrication	148:158	arg1	patches					243:249	developing epithelial patches	221:249	developing epithelial patches	221:249	Here we report fabrication of Gelatin-based biocomposite films and their application in developing epithelial patches.					
31597222	8	98	theme	junctional	1363:1372	arg1	ZO1					1382:1384	junctional protein ZO1	1363:1384	junctional protein ZO1	1363:1384	However, despite the expression of epithelial lineage markers, these cells did not form fully functional tight junctions as evidenced by low expression of junctional protein ZO1.					
31597222	2	99	theme	respiratory	402:412	arg1	epithelium					414:423	organized respiratory epithelium	392:423	organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs)	392:480	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	0	100	theme	composite	2:10	arg1	hydrogel					36:43	A composite Gelatin/hyaluronic acid hydrogel	0:43	A composite Gelatin/hyaluronic acid hydrogel as an ECM	0:53	A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell-derived epithelial tissue patches.					
31597222	2	101	theme	stem	464:467	arg1	MSCs					476:479	MSCs	476:479	MSCs	476:479	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	2	101	theme	stem	464:467	arg1	cells					469:473	mesenchymal stem cells	452:473	mesenchymal stem cells (MSCs)	452:480	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	10	102	from	possibility	1611:1621	arg1	engineering					1658:1668	epithelial tissue engineering	1640:1668	epithelial tissue engineering	1640:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	8	103	theme	ZO1	1382:1384	arg1	expression					1349:1358	low expression	1345:1358	low expression of junctional protein ZO1	1345:1384	However, despite the expression of epithelial lineage markers, these cells did not form fully functional tight junctions as evidenced by low expression of junctional protein ZO1.					
31597222	0	104	theme	acid	31:34	arg1	hydrogel					36:43	A composite Gelatin/hyaluronic acid hydrogel	0:43	A composite Gelatin/hyaluronic acid hydrogel as an ECM	0:53	A composite Gelatin/hyaluronic acid hydrogel as an ECM mimic for developing mesenchymal stem cell-derived epithelial tissue patches.					
31597222	2	105	dep	matrix	350:355	arg1	mimic					357:361	mimic	357:361	mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs)	357:480	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	2	106	theme	cell	438:441	arg1	line					443:446	Calu-3 cell line	431:446	Calu-3 cell line	431:446	The films were loaded with an epithelial cell growth factor cocktail and used as an extracellular matrix mimic for in vitro regeneration of organized respiratory epithelium using Calu-3 cell line and mesenchymal stem cells (MSCs).					
31597222	7	107	theme	cytokeratin	1157:1167	arg1	expression					1169:1178	pan cytokeratin expression	1153:1178	pan cytokeratin expression	1153:1178	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	10	108	with	matrix	1750:1755	arg1	modelling					1853:1861	in vitro modelling	1844:1861	in vitro modelling of barrier tissues	1844:1880	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	108	with	matrix	1750:1755	arg1	applications					1802:1813	potential applications	1792:1813	potential applications in regenerative medicine	1792:1838	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	109	theme	composite	1699:1707	arg1	hydrogels					1709:1717	the composite hydrogels	1695:1717	the composite hydrogels	1695:1717	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	110	theme	hydrogels	1709:1717	arg1	applicability					1678:1690	the applicability	1674:1690	the applicability of the composite hydrogels	1674:1717	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	110	theme	hydrogels	1709:1717	arg1	possibility					1611:1621	the possibility	1607:1621	the possibility of using MSCs in epithelial tissue engineering	1607:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	110	theme	hydrogels	1709:1717	arg1	platforms					1777:1785	delivery platforms	1768:1785	delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues	1768:1880	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	5	111	theme	biomimetic	789:798	arg1	scaffold					800:807	a biomimetic scaffold and growth factor	787:825	scaffold	800:807	We also show the suitability of the composite films as a biomimetic scaffold and growth factor delivery platform for differentiation of human MSCs to epithelial cells.					
31597222	10	112	from	applicability	1678:1690	arg1	engineering					1658:1668	epithelial tissue engineering	1640:1668	epithelial tissue engineering	1640:1668	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	7	113	with	weeks	1095:1099	arg1	reduction					1118:1126	significant reduction	1106:1126	significant reduction in vimentin	1106:1138	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	7	113	with	weeks	1095:1099	arg1	changes					1199:1205	morphological changes	1185:1205	morphological changes	1185:1205	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	7	113	with	weeks	1095:1099	arg1	increase					1141:1148	increase	1141:1148	increase in pan cytokeratin expression	1141:1178	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31597222	10	114	with	platforms	1777:1785	arg1	modelling					1853:1861	in vitro modelling	1844:1861	in vitro modelling of barrier tissues	1844:1880	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	10	114	with	platforms	1777:1785	arg1	applications					1802:1813	potential applications	1792:1813	potential applications in regenerative medicine	1792:1838	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	9	115	from	formation	1495:1503	arg1	cells					1532:1536	MSCs-derived epithelial cells	1508:1536	MSCs-derived epithelial cells	1508:1536	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	10	116	theme	potential	1792:1800	arg1	applications					1802:1813	potential applications	1792:1813	potential applications in regenerative medicine	1792:1838	Nevertheless, our data clearly highlight the possibility of using MSCs in epithelial tissue engineering and the applicability of the composite hydrogels as transferrable extracellular matrix mimics and delivery platforms with potential applications in regenerative medicine and in vitro modelling of barrier tissues.					
31597222	9	117	theme	culture	1411:1417	arg1	conditions					1419:1428	culture conditions	1411:1428	culture conditions	1411:1428	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	9	118	theme	MSCs-derived	1508:1519	arg1	cells					1532:1536	MSCs-derived epithelial cells	1508:1536	MSCs-derived epithelial cells	1508:1536	Further optimisation of culture conditions and growth factor cocktail is required to enhance tight junction formation in MSCs-derived epithelial cells on the composite hydrogels.					
31597222	7	119	theme	morphological	1185:1197	arg1	changes					1199:1205	morphological changes	1185:1205	morphological changes	1185:1205	Our data show that the MSCs acquire the epithelial characteristics after 2 weeks with significant reduction in vimentin, increase in pan cytokeratin expression, and morphological changes.					
31312254	6	0	theme	human	1057:1061	arg1	lactoferrin					1063:1073	the commercially purchased human lactoferrin	1030:1073	the commercially purchased human lactoferrin	1030:1073	The purified rhLF was detected with a secondary structure similar with the commercially purchased human lactoferrin.					
31312254	7	1	theme	N-glycans	1091:1099	arg1	types					1082:1086	Eight types	1076:1086	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%)	1076:1186	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	1	2	used	used	183:186	arg2	glands					172:177	BACKGROUND Silk glands	156:177	BACKGROUND Silk glands	156:177	BACKGROUND Silk glands are used by silkworms to spin silk fibers for making their cocoons.					
31312254	5	3	theme	rhLF	855:858	arg1	9.24 mg					840:846	9.24 mg	840:846	9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average	840:914	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	3	4	from	lactoferrin	510:520	arg1	gland					541:545	the silk gland	532:545	the silk gland	532:545	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	1	5	theme	spin	204:207	arg1	fibers					214:219	spin silk fibers	204:219	spin silk fibers	204:219	BACKGROUND Silk glands are used by silkworms to spin silk fibers for making their cocoons.					
31312254	7	6	theme	rhLF	1254:1257	arg1	sites					1241:1245	the three typical N-glycosylation sites	1207:1245	the three typical N-glycosylation sites of the rhLF	1207:1257	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	6	theme	rhLF	1254:1257	arg1	rhLF					1254:1257	the rhLF	1250:1257	the rhLF	1250:1257	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	0	7	theme	anti-inflammatory	126:142	arg1	activities					144:153	antibacterial and anti-inflammatory activities	108:153	antibacterial and anti-inflammatory activities	108:153	A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.					
31312254	9	8	theme	silk	1586:1589	arg1	bioreactor					1629:1638	an efficient bioreactor	1616:1638	an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial	1616:1739	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	9	8	theme	silk	1586:1589	arg1	gland					1591:1595	the middle silk gland	1575:1595	the middle silk gland of silkworm	1575:1607	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	3	9	theme	constructed	596:606	arg1	system					636:641	our previously constructed silk gland based bioreactor system	581:641	our previously constructed silk gland based bioreactor system	581:641	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	5	10	theme	simple	810:815	arg1	protocol					830:837	a simple purification protocol	808:837	a simple purification protocol	808:837	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	7	11	theme	N-glycosylation	1225:1239	arg1	sites					1241:1245	the three typical N-glycosylation sites	1207:1245	the three typical N-glycosylation sites of the rhLF	1207:1257	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	11	theme	N-glycosylation	1225:1239	arg1	rhLF					1254:1257	the rhLF	1250:1257	the rhLF	1250:1257	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	3	12	theme	bioreactor	625:634	arg1	system					636:641	our previously constructed silk gland based bioreactor system	581:641	our previously constructed silk gland based bioreactor system	581:641	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	5	13	theme	%	903:903	arg1	%					882:882	76.55%	877:882	76.55%	877:882	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	5	13	theme	%	903:903	arg1	purity					888:893	purity	888:893	purity	888:893	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	5	13	theme	%	903:903	arg1	%					903:903	95.45%	898:903	95.45% on average	898:914	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	4	14	theme	highest	675:681	arg1	level					694:698	the highest expression level	671:698	the highest expression level	671:698	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	3	15	theme	gland	613:617	arg1	system					636:641	our previously constructed silk gland based bioreactor system	581:641	our previously constructed silk gland based bioreactor system	581:641	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	6	16	theme	purified	963:970	arg1	rhLF					972:975	The purified rhLF	959:975	The purified rhLF	959:975	The purified rhLF was detected with a secondary structure similar with the commercially purchased human lactoferrin.					
31312254	0	17	theme	recombinant	73:83	arg1	lactoferrin					91:101	recombinant human lactoferrin	73:101	recombinant human lactoferrin	73:101	A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.					
31312254	5	18	from	%	882:882	arg1	average					908:914	average	908:914	average	908:914	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	3	19	dep	RESULTS	403:409	arg1	established					429:439	established	429:439	established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system	429:641	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	0	20	theme	lactoferrin	91:101	arg1	production					59:68	high-efficiency production	43:68	high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities	43:153	A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.					
31312254	1	21	theme	BACKGROUND	156:165	arg1	glands					172:177	BACKGROUND Silk glands	156:177	BACKGROUND Silk glands	156:177	BACKGROUND Silk glands are used by silkworms to spin silk fibers for making their cocoons.					
31312254	8	22	theme	-induced	1385:1392	arg1	inflammation					1399:1410	the lipopolysaccharide (LPS)-induced cell inflammation	1357:1410	the lipopolysaccharide (LPS)-induced cell inflammation	1357:1410	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	4	23	theme	transgenic	767:776	arg1	strain					787:792	the transgenic silkworm strain 34	763:795	the transgenic silkworm strain 34	763:795	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	3	24	theme	transgenic	443:452	arg1	strain					463:468	a transgenic silkworm strain	441:468	a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system	441:641	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	0	25	theme	antibacterial	108:120	arg1	activities					144:153	antibacterial and anti-inflammatory activities	108:153	antibacterial and anti-inflammatory activities	108:153	A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.					
31312254	9	26	theme	rhLF	1667:1670	arg1	production					1653:1662	the mass production	1644:1662	the mass production of rhLF	1644:1670	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	9	26	theme	rhLF	1667:1670	arg1	application					1690:1700	the potential application	1676:1700	the potential application in anti-inflammation and antibacterial	1676:1739	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	4	27	theme	cocoon	731:736	arg1	weight					744:749	12.07 mg/g cocoon shell weight	720:749	12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34	720:795	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	4	27	theme	cocoon	731:736	arg1	yield					648:652	The yield	644:652	The yield of the rhLF with the highest expression level	644:698	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	9	28	dep	CONCLUSIONS	1539:1549	arg1	show					1565:1568	show	1565:1568	show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial	1565:1739	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	5	29	from	purity	888:893	arg1	average					908:914	average	908:914	average	908:914	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	6	30	theme	similar	1017:1023	arg1	structure					1007:1015	a secondary structure	995:1015	a secondary structure similar with the commercially purchased human lactoferrin	995:1073	The purified rhLF was detected with a secondary structure similar with the commercially purchased human lactoferrin.					
31312254	8	31	theme	RAW264.7	1415:1422	arg1	cells					1424:1428	RAW264.7 cells	1415:1428	RAW264.7 cells	1415:1428	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	5	32	with	9.24 mg	840:846	arg1	recovery					865:872	recovery	865:872	recovery of 76.55% and purity of 95.45% on average	865:914	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	5	33	theme	purification	817:828	arg1	protocol					830:837	a simple purification protocol	808:837	a simple purification protocol	808:837	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	3	34	theme	silk	536:539	arg1	gland					541:545	the silk gland	532:545	the silk gland	532:545	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	9	35	from	production	1653:1662	arg1	antibacterial					1727:1739	antibacterial	1727:1739	antibacterial	1727:1739	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	9	35	from	production	1653:1662	arg1	anti-inflammation					1705:1721	anti-inflammation	1705:1721	anti-inflammation	1705:1721	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	6	36	theme	secondary	997:1005	arg1	structure					1007:1015	a secondary structure	995:1015	a secondary structure similar with the commercially purchased human lactoferrin	995:1073	The purified rhLF was detected with a secondary structure similar with the commercially purchased human lactoferrin.					
31312254	0	37	theme	gland	22:26	arg1	bioreactor					28:37	silk gland bioreactor	17:37	silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities	17:153	A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.					
31312254	8	38	theme	potent	1442:1447	arg1	bioactivities					1463:1475	potent antibacterial bioactivities	1442:1475	potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis	1442:1536	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	8	39	theme	activities	1271:1280	arg1	assays					1282:1287	Biological activities assays	1260:1287	Biological activities assays	1260:1287	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	2	40	theme	proteins	363:370	arg1	production					324:333	the cost-effective production	305:333	the cost-effective production of other valuable exogenous proteins	305:370	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	2	41	theme	valuable	344:351	arg1	proteins					363:370	other valuable exogenous proteins	338:370	other valuable exogenous proteins	338:370	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	5	42	theme	cocoons	950:956	arg1	1 g					939:941	1 g	939:941	1 g of the cocoons	939:956	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	8	43	theme	significant	1300:1310	arg1	evidence					1312:1319	the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis	1296:1536	the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis	1296:1536	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	2	44	theme	wide	387:390	arg1	hosts					295:299	bioreactor hosts	284:299	bioreactor hosts for the cost-effective production of other valuable exogenous proteins	284:370	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	2	44	theme	wide	387:390	arg1	attention					392:400	wide attention	387:400	wide attention	387:400	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	2	44	theme	wide	387:390	arg1	These					247:251	These	247:251	These	247:251	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	5	45	from	average	908:914	arg1	%					882:882	76.55%	877:882	76.55%	877:882	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	5	45	from	average	908:914	arg1	purity					888:893	purity	888:893	purity	888:893	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	5	45	from	average	908:914	arg1	%					903:903	95.45%	898:903	95.45% on average	898:914	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	9	46	from	application	1690:1700	arg1	antibacterial					1727:1739	antibacterial	1727:1739	antibacterial	1727:1739	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	9	46	from	application	1690:1700	arg1	anti-inflammation					1705:1721	anti-inflammation	1705:1721	anti-inflammation	1705:1721	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	6	47	theme	purchased	1047:1055	arg1	lactoferrin					1063:1073	the commercially purchased human lactoferrin	1030:1073	the commercially purchased human lactoferrin	1030:1073	The purified rhLF was detected with a secondary structure similar with the commercially purchased human lactoferrin.					
31312254	0	48	with	production	59:68	arg1	activities					144:153	antibacterial and anti-inflammatory activities	108:153	antibacterial and anti-inflammatory activities	108:153	A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.					
31312254	2	49	theme	bioreactor	284:293	arg1	hosts					295:299	bioreactor hosts	284:299	bioreactor hosts for the cost-effective production of other valuable exogenous proteins	284:370	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	2	49	theme	bioreactor	284:293	arg1	attention					392:400	wide attention	387:400	wide attention	387:400	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	2	49	theme	bioreactor	284:293	arg1	These					247:251	These	247:251	These	247:251	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	4	50	theme	rhLF	661:664	arg1	yield					648:652	The yield	644:652	The yield of the rhLF with the highest expression level	644:698	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	4	50	theme	rhLF	661:664	arg1	weight					744:749	12.07 mg/g cocoon shell weight	720:749	12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34	720:795	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	2	51	theme	cost-effective	309:322	arg1	production					324:333	the cost-effective production	305:333	the cost-effective production of other valuable exogenous proteins	305:370	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	1	52	theme	silk	209:212	arg1	fibers					214:219	spin silk fibers	204:219	spin silk fibers	204:219	BACKGROUND Silk glands are used by silkworms to spin silk fibers for making their cocoons.					
31312254	9	53	theme	middle	1579:1584	arg1	bioreactor					1629:1638	an efficient bioreactor	1616:1638	an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial	1616:1739	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	9	53	theme	middle	1579:1584	arg1	gland					1591:1595	the middle silk gland	1575:1595	the middle silk gland of silkworm	1575:1607	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	8	54	theme	purified	1330:1337	arg1	rhLF					1339:1342	the purified rhLF	1326:1342	the purified rhLF	1326:1342	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	5	55	theme	purity	888:893	arg1	recovery					865:872	recovery	865:872	recovery of 76.55% and purity of 95.45% on average	865:914	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	7	56	gly	N-glycosylation	1225:1239	arg2	rhLF					1254:1257	the rhLF	1250:1257	the rhLF	1250:1257	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	56	gly	N-glycosylation	1225:1239	arg2	sites					1241:1245	the three typical N-glycosylation sites	1207:1245	the three typical N-glycosylation sites of the rhLF	1207:1257	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	56	gly	N-glycosylation	1225:1239	arg1	rhLF					1254:1257	the rhLF	1250:1257	the rhLF	1250:1257	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	56	gly	N-glycosylation	1225:1239	arg2	three					1211:1215	three	1211:1215	three	1211:1215	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	9	57	theme	silkworm	1600:1607	arg1	bioreactor					1629:1638	an efficient bioreactor	1616:1638	an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial	1616:1739	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	9	57	theme	silkworm	1600:1607	arg1	gland					1591:1595	the middle silk gland	1575:1595	the middle silk gland of silkworm	1575:1607	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	7	58	theme	GlcNAc	1124:1129	arg1	3					1140:1140	3	1140:1140	3	1140:1140	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	58	theme	GlcNAc	1124:1129	arg1	Man					1135:1137	the GlcNAc (4) Man	1120:1137	the GlcNAc (4) Man (3) (61.15%)	1120:1150	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	58	theme	GlcNAc	1124:1129	arg1	%					1149:1149	61.15%	1144:1149	61.15%	1144:1149	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	3	59	theme	based	619:623	arg1	system					636:641	our previously constructed silk gland based bioreactor system	581:641	our previously constructed silk gland based bioreactor system	581:641	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	7	60	theme	typical	1217:1223	arg1	sites					1241:1245	the three typical N-glycosylation sites	1207:1245	the three typical N-glycosylation sites of the rhLF	1207:1257	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	60	theme	typical	1217:1223	arg1	rhLF					1254:1257	the rhLF	1250:1257	the rhLF	1250:1257	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	3	61	theme	recombinant	492:502	arg1	lactoferrin					510:520	the recombinant human lactoferrin	488:520	the recombinant human lactoferrin (rhLF) in the silk gland	488:545	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	3	61	theme	recombinant	492:502	arg1	rhLF					523:526	rhLF	523:526	rhLF	523:526	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	3	62	theme	silk	608:611	arg1	system					636:641	our previously constructed silk gland based bioreactor system	581:641	our previously constructed silk gland based bioreactor system	581:641	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	1	63	theme	Silk	167:170	arg1	glands					172:177	BACKGROUND Silk glands	156:177	BACKGROUND Silk glands	156:177	BACKGROUND Silk glands are used by silkworms to spin silk fibers for making their cocoons.					
31312254	9	64	theme	efficient	1619:1627	arg1	bioreactor					1629:1638	an efficient bioreactor	1616:1638	an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial	1616:1739	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	9	64	theme	efficient	1619:1627	arg1	gland					1591:1595	the middle silk gland	1575:1595	the middle silk gland of silkworm	1575:1607	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	8	65	from	relief	1350:1355	arg1	cells					1424:1428	RAW264.7 cells	1415:1428	RAW264.7 cells	1415:1428	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	0	66	theme	human	85:89	arg1	lactoferrin					91:101	recombinant human lactoferrin	73:101	recombinant human lactoferrin	73:101	A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.					
31312254	5	67	theme	%	882:882	arg1	recovery					865:872	recovery	865:872	recovery of 76.55% and purity of 95.45% on average	865:914	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	4	68	theme	expression	683:692	arg1	level					694:698	the highest expression level	671:698	the highest expression level	671:698	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	9	69	theme	mass	1648:1651	arg1	production					1653:1662	the mass production	1644:1662	the mass production of rhLF	1644:1670	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	8	70	theme	cell	1394:1397	arg1	inflammation					1399:1410	the lipopolysaccharide (LPS)-induced cell inflammation	1357:1410	the lipopolysaccharide (LPS)-induced cell inflammation	1357:1410	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	3	71	theme	human	504:508	arg1	lactoferrin					510:520	the recombinant human lactoferrin	488:520	the recombinant human lactoferrin (rhLF) in the silk gland	488:545	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	3	71	theme	human	504:508	arg1	rhLF					523:526	rhLF	523:526	rhLF	523:526	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	8	72	dep	coli	1501:1504	arg1	coli					1510:1513	E. coli	1507:1513	E. coli	1507:1513	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	3	73	theme	silkworm	454:461	arg1	strain					463:468	a transgenic silkworm strain	441:468	a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system	441:641	RESULTS In this study, we established a transgenic silkworm strain which synthesizes the recombinant human lactoferrin (rhLF) in the silk gland and spins them into the cocoon by our previously constructed silk gland based bioreactor system.					
31312254	5	74	from	%	903:903	arg1	average					908:914	average	908:914	average	908:914	Utilizing a simple purification protocol, 9.24 mg of the rhLF with recovery of 76.55% and purity of 95.45% on average could be purified from 1 g of the cocoons.					
31312254	4	75	theme	12.07 mg/g	720:729	arg1	weight					744:749	12.07 mg/g cocoon shell weight	720:749	12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34	720:795	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	4	75	theme	12.07 mg/g	720:729	arg1	yield					648:652	The yield	644:652	The yield of the rhLF with the highest expression level	644:698	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	0	76	theme	silk	17:20	arg1	bioreactor					28:37	silk gland bioreactor	17:37	silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities	17:153	A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.					
31312254	9	77	theme	potential	1680:1688	arg1	application					1690:1700	the potential application	1676:1700	the potential application in anti-inflammation and antibacterial	1676:1739	CONCLUSIONS These results show that the middle silk gland of silkworm can be an efficient bioreactor for the mass production of rhLF and the potential application in anti-inflammation and antibacterial.					
31312254	4	78	theme	shell	738:742	arg1	weight					744:749	12.07 mg/g cocoon shell weight	720:749	12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34	720:795	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	4	78	theme	shell	738:742	arg1	yield					648:652	The yield	644:652	The yield of the rhLF with the highest expression level	644:698	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	6	79	with	similar	1017:1023	arg1	lactoferrin					1063:1073	the commercially purchased human lactoferrin	1030:1073	the commercially purchased human lactoferrin	1030:1073	The purified rhLF was detected with a secondary structure similar with the commercially purchased human lactoferrin.					
31312254	8	80	theme	antibacterial	1449:1461	arg1	bioactivities					1463:1475	potent antibacterial bioactivities	1442:1475	potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis	1442:1536	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	0	81	theme	high-efficiency	43:57	arg1	production					59:68	high-efficiency production	43:68	high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities	43:153	A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities.					
31312254	4	82	with	yield	648:652	arg1	level					694:698	the highest expression level	671:698	the highest expression level	671:698	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	8	83	theme	Biological	1260:1269	arg1	activities					1271:1280	Biological activities	1260:1280	Biological activities assays	1260:1287	Biological activities assays showed the significant evidence that the purified rhLF could relief the lipopolysaccharide (LPS)-induced cell inflammation in RAW264.7 cells and exhibit potent antibacterial bioactivities against the Escherichia coli (E. coli) and Bacillus subtilis.					
31312254	2	84	theme	exogenous	353:361	arg1	proteins					363:370	other valuable exogenous proteins	338:370	other valuable exogenous proteins	338:370	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	4	85	theme	silkworm	778:785	arg1	strain					787:792	the transgenic silkworm strain 34	763:795	the transgenic silkworm strain 34	763:795	The yield of the rhLF with the highest expression level was estimated to be 12.07 mg/g cocoon shell weight produced by the transgenic silkworm strain 34.					
31312254	2	86	theme	other	338:342	arg1	proteins					363:370	other valuable exogenous proteins	338:370	other valuable exogenous proteins	338:370	These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and have drawn wide attention.					
31312254	7	87	theme	GlcNAc	1160:1165	arg1	3					1176:1176	3	1176:1176	3	1176:1176	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	87	theme	GlcNAc	1160:1165	arg1	Man					1171:1173	the GlcNAc (3) Man	1156:1173	the GlcNAc (3) Man (3) (17.98%)	1156:1186	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
31312254	7	87	theme	GlcNAc	1160:1165	arg1	%					1185:1185	17.98%	1180:1185	17.98%	1180:1185	Eight types of N-glycans which dominated by the GlcNAc (4) Man (3) (61.15%) and the GlcNAc (3) Man (3) (17.98%) were identified at the three typical N-glycosylation sites of the rhLF.					
29889351	12	0	theme	different	1546:1554	arg1	impacts					1570:1576	some different physiological impacts	1541:1576	some different physiological impacts	1541:1576	Nevertheless, some different physiological impacts were observed.					
29889351	0	1	from	Origins	35:41	arg1	Propolis					0:7	Propolis	0:7	Propolis from Different Geographic Origins	0:41	Propolis from Different Geographic Origins Decreases Intestinal Inflammation and Bacteroides spp.					
29889351	8	2	theme	inflammatory	1113:1124	arg1	markers					1126:1132	Colonic inflammatory markers	1105:1132	Colonic inflammatory markers IL-1β, IL-6, and MCP-1	1105:1155	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	2	theme	inflammatory	1113:1124	arg1	IL-6					1141:1144	IL-6	1141:1144	IL-6	1141:1144	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	2	theme	inflammatory	1113:1124	arg1	MCP-1					1151:1155	MCP-1	1151:1155	MCP-1	1151:1155	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	2	theme	inflammatory	1113:1124	arg1	IL-1β					1134:1138	IL-1β	1134:1138	IL-1β	1134:1138	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	6	3	theme	reduced	985:991	arg1	levels					1007:1012	reduced malonaldehyde levels	985:1012	reduced malonaldehyde levels	985:1012	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	6	4	theme	tissue	893:898	arg1	damage					900:905	significant DSS-induced colonic tissue damage	861:905	significant DSS-induced colonic tissue damage	861:905	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	7	5	theme	DSS-induced	1074:1084	arg1	apoptosis					1094:1102	DSS-induced colonic apoptosis	1074:1102	DSS-induced colonic apoptosis	1074:1102	CP and BP significantly reduced DSS-induced colonic apoptosis.					
29889351	6	6	theme	disease	783:789	arg1	index					800:804	the colitis disease activity index	771:804	the colitis disease activity index relative to controls not receiving propolis	771:848	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	4	7	theme	RESULTS	473:479	arg1	analysis					499:506	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis	461:506	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis	461:506	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis confirmed that polyphenol compositions of CP and BP were dissimilar.					
29889351	4	8	theme	CP	550:551	arg1	compositions					534:545	polyphenol compositions	523:545	polyphenol compositions of CP and BP	523:558	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis confirmed that polyphenol compositions of CP and BP were dissimilar.					
29889351	5	9	theme	300	613:615	arg1	mg					617:618	mg	617:618	mg	617:618	Rats were given CP or BP by gavage (300 mg kg-1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS.					
29889351	11	10	theme	DSS-induced	1495:1505	arg1	colitis					1507:1513	DSS-induced colitis	1495:1513	DSS-induced colitis	1495:1513	CONCLUSIONS Despite the dissimilar polyphenol compositions of CP and BP, their ability to protect against DSS-induced colitis is similar.					
29889351	6	11	theme	DSS-induced	873:883	arg1	damage					900:905	significant DSS-induced colonic tissue damage	861:905	significant DSS-induced colonic tissue damage	861:905	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	5	12	theme	mg	617:618	arg1	gavage					605:610	gavage	605:610	gavage (300 mg kg-1 body weight)	605:636	Rats were given CP or BP by gavage (300 mg kg-1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS.					
29889351	5	12	theme	mg	617:618	arg1	weight					630:635	300 mg kg-1 body weight	613:635	300 mg kg-1 body weight	613:635	Rats were given CP or BP by gavage (300 mg kg-1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS.					
29889351	11	13	theme	polyphenol	1424:1433	arg1	compositions					1435:1446	the dissimilar polyphenol compositions	1409:1446	the dissimilar polyphenol compositions of CP and BP	1409:1459	CONCLUSIONS Despite the dissimilar polyphenol compositions of CP and BP, their ability to protect against DSS-induced colitis is similar.					
29889351	6	14	theme	significant	861:871	arg1	damage					900:905	significant DSS-induced colonic tissue damage	861:905	significant DSS-induced colonic tissue damage	861:905	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	5	15	theme	body	625:628	arg1	gavage					605:610	gavage	605:610	gavage (300 mg kg-1 body weight)	605:636	Rats were given CP or BP by gavage (300 mg kg-1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS.					
29889351	5	15	theme	body	625:628	arg1	weight					630:635	300 mg kg-1 body weight	613:635	300 mg kg-1 body weight	613:635	Rats were given CP or BP by gavage (300 mg kg-1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS.					
29889351	5	16	theme	kg-1	620:623	arg1	gavage					605:610	gavage	605:610	gavage (300 mg kg-1 body weight)	605:636	Rats were given CP or BP by gavage (300 mg kg-1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS.					
29889351	5	16	theme	kg-1	620:623	arg1	weight					630:635	300 mg kg-1 body weight	613:635	300 mg kg-1 body weight	613:635	Rats were given CP or BP by gavage (300 mg kg-1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS.					
29889351	8	17	theme	TGF-β	1225:1229	arg1	expression					1211:1220	only BP-induced expression	1195:1220	only BP-induced expression of TGF-β	1195:1229	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	18	theme	BP-induced	1200:1209	arg1	expression					1211:1220	only BP-induced expression	1195:1220	only BP-induced expression of TGF-β	1195:1229	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	2	19	theme	Dietary	151:157	arg1	supplementation					159:173	SCOPE Dietary supplementation	145:173	SCOPE Dietary supplementation with polyphenol-rich propolis	145:203	SCOPE Dietary supplementation with polyphenol-rich propolis can protect against experimentally induced colitis.					
29889351	11	20	theme	dissimilar	1413:1422	arg1	compositions					1435:1446	the dissimilar polyphenol compositions	1409:1446	the dissimilar polyphenol compositions of CP and BP	1409:1459	CONCLUSIONS Despite the dissimilar polyphenol compositions of CP and BP, their ability to protect against DSS-induced colitis is similar.					
29889351	2	21	theme	SCOPE	145:149	arg1	supplementation					159:173	SCOPE Dietary supplementation	145:173	SCOPE Dietary supplementation with polyphenol-rich propolis	145:203	SCOPE Dietary supplementation with polyphenol-rich propolis can protect against experimentally induced colitis.					
29889351	3	22	theme	different	277:285	arg1	compositions					298:309	different polyphenol compositions	277:309	different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP)	277:362	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	9	23	dep	diversity	1258:1266	arg1	the					1254:1256	the	1254:1256	the	1254:1256	CP, not BP, increased the diversity and richness of gut microbiota populations.					
29889351	8	24	theme	Colonic	1105:1111	arg1	markers					1126:1132	Colonic inflammatory markers	1105:1132	Colonic inflammatory markers IL-1β, IL-6, and MCP-1	1105:1155	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	24	theme	Colonic	1105:1111	arg1	IL-6					1141:1144	IL-6	1141:1144	IL-6	1141:1144	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	24	theme	Colonic	1105:1111	arg1	MCP-1					1151:1155	MCP-1	1151:1155	MCP-1	1151:1155	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	24	theme	Colonic	1105:1111	arg1	IL-1β					1134:1138	IL-1β	1134:1138	IL-1β	1134:1138	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	25	dep	markers	1126:1132	arg1	markers					1126:1132	Colonic inflammatory markers	1105:1132	Colonic inflammatory markers IL-1β, IL-6, and MCP-1	1105:1155	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	25	dep	markers	1126:1132	arg1	IL-6					1141:1144	IL-6	1141:1144	IL-6	1141:1144	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	25	dep	markers	1126:1132	arg1	MCP-1					1151:1155	MCP-1	1151:1155	MCP-1	1151:1155	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	8	25	dep	markers	1126:1132	arg1	IL-1β					1134:1138	IL-1β	1134:1138	IL-1β	1134:1138	Colonic inflammatory markers IL-1β, IL-6, and MCP-1 were suppressed by CP and BP, whereas only BP-induced expression of TGF-β.					
29889351	9	26	theme	populations	1299:1309	arg1	diversity					1258:1266	diversity	1258:1266	diversity	1258:1266	CP, not BP, increased the diversity and richness of gut microbiota populations.					
29889351	9	26	theme	populations	1299:1309	arg1	richness					1272:1279	richness	1272:1279	richness	1272:1279	CP, not BP, increased the diversity and richness of gut microbiota populations.					
29889351	3	27	theme	polyphenol	287:296	arg1	compositions					298:309	different polyphenol compositions	277:309	different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP)	277:362	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	11	28	theme	BP	1458:1459	arg1	compositions					1435:1446	the dissimilar polyphenol compositions	1409:1446	the dissimilar polyphenol compositions of CP and BP	1409:1459	CONCLUSIONS Despite the dissimilar polyphenol compositions of CP and BP, their ability to protect against DSS-induced colitis is similar.					
29889351	0	29	theme	Geographic	24:33	arg1	Origins					35:41	Different Geographic Origins	14:41	Different Geographic Origins	14:41	Propolis from Different Geographic Origins Decreases Intestinal Inflammation and Bacteroides spp.					
29889351	6	30	theme	oxidative	956:964	arg1	stress					966:971	DSS-induced colonic oxidative stress	936:971	DSS-induced colonic oxidative stress	936:971	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	5	31	theme	DSS	685:687	arg1	treatment					689:697	DSS treatment	685:697	DSS treatment	685:697	Rats were given CP or BP by gavage (300 mg kg-1 body weight) throughout the study, starting 1 week prior to DSS treatment for 1 week followed by 3 d without DSS.					
29889351	10	32	theme	spp	1384:1386	arg1	populations					1357:1367	populations	1357:1367	populations of Bacteroides spp	1357:1386	Both forms of propolis significantly reduced populations of Bacteroides spp.					
29889351	6	33	theme	colonic	885:891	arg1	damage					900:905	significant DSS-induced colonic tissue damage	861:905	significant DSS-induced colonic tissue damage	861:905	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	0	34	theme	Different	14:22	arg1	Origins					35:41	Different Geographic Origins	14:41	Different Geographic Origins	14:41	Propolis from Different Geographic Origins Decreases Intestinal Inflammation and Bacteroides spp.					
29889351	6	35	theme	colonic	948:954	arg1	stress					966:971	DSS-induced colonic oxidative stress	936:971	DSS-induced colonic oxidative stress	936:971	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	11	36	theme	CP	1451:1452	arg1	compositions					1435:1446	the dissimilar polyphenol compositions	1409:1446	the dissimilar polyphenol compositions of CP and BP	1409:1459	CONCLUSIONS Despite the dissimilar polyphenol compositions of CP and BP, their ability to protect against DSS-induced colitis is similar.					
29889351	6	37	theme	DSS-induced	936:946	arg1	stress					966:971	DSS-induced colonic oxidative stress	936:971	DSS-induced colonic oxidative stress	936:971	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	3	38	theme	Chinese	314:320	arg1	CP					332:333	CP	332:333	CP	332:333	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	3	38	theme	Chinese	314:320	arg1	propolis					322:329	Chinese propolis	314:329	Chinese propolis (CP)	314:334	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	4	39	theme	METHODS	461:467	arg1	analysis					499:506	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis	461:506	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis	461:506	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis confirmed that polyphenol compositions of CP and BP were dissimilar.					
29889351	3	40	theme	-induced	435:442	arg1	colitis					444:450	dextran sulfate sodium (DSS)-induced colitis	407:450	dextran sulfate sodium (DSS)-induced colitis in rats	407:458	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	3	41	theme	propolis	322:329	arg1	compositions					298:309	different polyphenol compositions	277:309	different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP)	277:362	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	4	42	theme	HPLC-DAD/Q-TOF-MS	481:497	arg1	analysis					499:506	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis	461:506	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis	461:506	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis confirmed that polyphenol compositions of CP and BP were dissimilar.					
29889351	2	43	with	supplementation	159:173	arg1	propolis					196:203	polyphenol-rich propolis	180:203	polyphenol-rich propolis	180:203	SCOPE Dietary supplementation with polyphenol-rich propolis can protect against experimentally induced colitis.					
29889351	4	44	theme	BP	557:558	arg1	compositions					534:545	polyphenol compositions	523:545	polyphenol compositions of CP and BP	523:558	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis confirmed that polyphenol compositions of CP and BP were dissimilar.					
29889351	3	45	theme	Brazilian	340:348	arg1	BP					360:361	BP	360:361	BP	360:361	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	3	45	theme	Brazilian	340:348	arg1	propolis					350:357	Brazilian propolis	340:357	Brazilian propolis (BP)	340:362	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	2	46	theme	polyphenol-rich	180:194	arg1	propolis					196:203	polyphenol-rich propolis	180:203	polyphenol-rich propolis	180:203	SCOPE Dietary supplementation with polyphenol-rich propolis can protect against experimentally induced colitis.					
29889351	4	47	theme	polyphenol	523:532	arg1	compositions					534:545	polyphenol compositions	523:545	polyphenol compositions of CP and BP	523:558	METHODS AND RESULTS HPLC-DAD/Q-TOF-MS analysis confirmed that polyphenol compositions of CP and BP were dissimilar.					
29889351	0	48	theme	Intestinal	53:62	arg1	Inflammation					64:75	Intestinal Inflammation	53:75	Intestinal Inflammation	53:75	Propolis from Different Geographic Origins Decreases Intestinal Inflammation and Bacteroides spp.					
29889351	10	49	theme	Bacteroides	1372:1382	arg1	spp					1384:1386	Bacteroides spp	1372:1386	Bacteroides spp	1372:1386	Both forms of propolis significantly reduced populations of Bacteroides spp.					
29889351	3	50	theme	propolis	350:357	arg1	compositions					298:309	different polyphenol compositions	277:309	different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP)	277:362	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	6	51	theme	relative	806:813	arg1	index					800:804	the colitis disease activity index	771:804	the colitis disease activity index relative to controls not receiving propolis	771:848	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	1	52	theme	DSS-Induced	124:134	arg1	Colitis					136:142	DSS-Induced Colitis	124:142	DSS-Induced Colitis	124:142	Populations in a Model of DSS-Induced Colitis.					
29889351	9	53	theme	gut	1284:1286	arg1	populations					1299:1309	gut microbiota populations	1284:1309	gut microbiota populations	1284:1309	CP, not BP, increased the diversity and richness of gut microbiota populations.					
29889351	2	54	theme	induced	240:246	arg1	colitis					248:254	experimentally induced colitis	225:254	experimentally induced colitis	225:254	SCOPE Dietary supplementation with polyphenol-rich propolis can protect against experimentally induced colitis.					
29889351	11	55	dep	CONCLUSIONS	1389:1399	arg1	compositions					1435:1446	the dissimilar polyphenol compositions	1409:1446	the dissimilar polyphenol compositions of CP and BP	1409:1459	CONCLUSIONS Despite the dissimilar polyphenol compositions of CP and BP, their ability to protect against DSS-induced colitis is similar.					
29889351	6	56	theme	colitis	775:781	arg1	index					800:804	the colitis disease activity index	771:804	the colitis disease activity index relative to controls not receiving propolis	771:848	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	1	57	theme	Colitis	136:142	arg1	Model					115:119	a Model	113:119	a Model of DSS-Induced Colitis	113:142	Populations in a Model of DSS-Induced Colitis.					
29889351	6	58	theme	increased	1018:1026	arg1	levels					1034:1039	increased T-AOC levels	1018:1039	increased T-AOC levels	1018:1039	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	12	59	dep	different	1546:1554	arg1	physiological					1556:1568	physiological	1556:1568	physiological	1556:1568	Nevertheless, some different physiological impacts were observed.					
29889351	3	60	from	colitis	444:450	arg1	rats					455:458	rats	455:458	rats	455:458	We examined whether different polyphenol compositions of Chinese propolis (CP) and Brazilian propolis (BP) influence their ability to protect against dextran sulfate sodium (DSS)-induced colitis in rats.					
29889351	1	61	from	Populations	98:108	arg1	Model					115:119	a Model	113:119	a Model of DSS-Induced Colitis	113:142	Populations in a Model of DSS-Induced Colitis.					
29889351	7	62	theme	colonic	1086:1092	arg1	apoptosis					1094:1102	DSS-induced colonic apoptosis	1074:1102	DSS-induced colonic apoptosis	1074:1102	CP and BP significantly reduced DSS-induced colonic apoptosis.					
29889351	0	63	theme	Bacteroides	81:91	arg1	spp					93:95	Bacteroides spp	81:95	Bacteroides spp	81:95	Propolis from Different Geographic Origins Decreases Intestinal Inflammation and Bacteroides spp.					
29889351	6	64	theme	T-AOC	1028:1032	arg1	levels					1034:1039	increased T-AOC levels	1018:1039	increased T-AOC levels	1018:1039	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	6	65	theme	activity	791:798	arg1	index					800:804	the colitis disease activity index	771:804	the colitis disease activity index relative to controls not receiving propolis	771:848	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29889351	10	66	theme	propolis	1326:1333	arg1	forms					1317:1321	Both forms	1312:1321	Both forms of propolis	1312:1333	Both forms of propolis significantly reduced populations of Bacteroides spp.					
29889351	9	67	theme	microbiota	1288:1297	arg1	populations					1299:1309	gut microbiota populations	1284:1309	gut microbiota populations	1284:1309	CP, not BP, increased the diversity and richness of gut microbiota populations.					
29889351	6	68	theme	malonaldehyde	993:1005	arg1	levels					1007:1012	reduced malonaldehyde levels	985:1012	reduced malonaldehyde levels	985:1012	CP and BP significantly reduced the colitis disease activity index relative to controls not receiving propolis, prevented significant DSS-induced colonic tissue damage, and increased resistance to DSS-induced colonic oxidative stress as shown by reduced malonaldehyde levels and increased T-AOC levels.					
29701574	11	0	theme	53.5 mol	1287:1294	arg1	G+C content					1235:1245	The DNA G+C content	1227:1245	The DNA G+C content of strain THG-YT1T	1227:1264	The DNA G+C content of strain THG-YT1T was determined to be 53.5 mol%.					
29701574	11	0	theme	53.5 mol	1287:1294	arg1	%					1295:1295	53.5 mol%	1287:1295	53.5 mol%	1287:1295	The DNA G+C content of strain THG-YT1T was determined to be 53.5 mol%.					
29701574	12	1	theme	Bacillaceae	1404:1414	arg1	genus					1384:1388	a new genus	1378:1388	a new genus of the family Bacillaceae	1378:1414	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	7	2	theme	unidentified	886:897	arg1	aminophospholipids					899:916	two unidentified aminophospholipids	882:916	two unidentified aminophospholipids	882:916	The polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified aminolipids and two unidentified glycolipids.					
29701574	7	2	theme	unidentified	886:897	arg1	phosphatidylethanolamine					856:879	phosphatidylethanolamine	856:879	phosphatidylethanolamine	856:879	The polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified aminolipids and two unidentified glycolipids.					
29701574	4	3	theme	rRNA	384:387	arg1	sequences					394:402	16S rRNA gene sequences	380:402	16S rRNA gene sequences	380:402	Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae.					
29701574	4	4	from	genera	513:518	arg1	Bacillaceae					534:544	the family Bacillaceae	523:544	the family Bacillaceae	523:544	Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae.					
29701574	2	5	theme	Gram-stain-positive	108:126	arg1	THG-YT1T					226:233	THG-YT1T	226:233	THG-YT1T	226:233	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	2	5	theme	Gram-stain-positive	108:126	arg1	bacterium					215:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium	106:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T)	106:234	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	12	6	theme	genus	1384:1388	arg1	species					1367:1373	a novel species	1359:1373	a novel species	1359:1373	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	5	7	theme	%	805:805	arg1	genera					780:785	these genera	774:785	these genera of less than 95.0 %	774:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	2	8	attach	isolated	240:247	arg2	THG-YT1T					226:233	THG-YT1T	226:233	THG-YT1T	226:233	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	2	8	attach	isolated	240:247	arg2	bacterium					215:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium	106:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T)	106:234	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	2	8	attach	isolated	240:247	arg1	tea					260:262	green tea	254:262	green tea	254:262	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	12	9	theme	novel	1361:1365	arg1	species					1367:1373	a novel species	1359:1373	a novel species	1359:1373	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	11	10	theme	THG-YT1T	1257:1264	arg1	G+C content					1235:1245	The DNA G+C content	1227:1245	The DNA G+C content of strain THG-YT1T	1227:1264	The DNA G+C content of strain THG-YT1T was determined to be 53.5 mol%.					
29701574	11	10	theme	THG-YT1T	1257:1264	arg1	%					1295:1295	53.5 mol%	1287:1295	53.5 mol%	1287:1295	The DNA G+C content of strain THG-YT1T was determined to be 53.5 mol%.					
29701574	4	11	theme	related	505:511	arg1	genera					513:518	closely related genera	497:518	closely related genera in the family Bacillaceae	497:544	Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae.					
29701574	3	12	dep	optimum	294:300	arg1	25-30 °C					303:310	25-30 °C	303:310	25-30 °C	303:310	Growth occurred at 10-40 °C (optimum, 25-30 °C), at pH 6-8 (optimum, 7) and at 0-2 % NaCl (optimum, 0 %).					
29701574	2	13	theme	endospore-forming	161:177	arg1	THG-YT1T					226:233	THG-YT1T	226:233	THG-YT1T	226:233	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	2	13	theme	endospore-forming	161:177	arg1	bacterium					215:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium	106:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T)	106:234	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	14	14	theme	cellulosilyticus	1539:1554	arg1	strain					1512:1517	The type strain	1503:1517	The type strain	1503:1517	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	14	14	theme	cellulosilyticus	1539:1554	arg1	1.16306T					1584:1591	=KACC 19471T=CGMCC 1.16306T	1565:1591	=KACC 19471T=CGMCC 1.16306T	1565:1591	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	14	14	theme	cellulosilyticus	1539:1554	arg1	THG-YT1T					1556:1563	Camelliibacillus cellulosilyticus THG-YT1T	1522:1563	Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T)	1522:1592	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	4	15	theme	16S	380:382	arg1	sequences					394:402	16S rRNA gene sequences	380:402	16S rRNA gene sequences	380:402	Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae.					
29701574	8	16	theme	major	986:990	arg1	C18 					1028:1031	C18 	1028:1031	C18 : 3ω7c and anteiso-C17 : 0	1028:1057	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	8	16	theme	major	986:990	arg1	acids					998:1002	The major fatty acids	982:1002	The major fatty acids of strain THG-YT1T	982:1021	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	9	17	theme	meso-diaminopimelic	1106:1124	arg1	acid					1126:1129	meso-diaminopimelic acid	1106:1129	meso-diaminopimelic acid	1106:1129	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	1	18	theme	cellulose-degrading	57:75	arg1	bacterium					77:85	a cellulose-degrading bacterium	55:85	a cellulose-degrading bacterium isolated from tea	55:103	nov., a cellulose-degrading bacterium isolated from tea.					
29701574	5	19	theme	gene	715:718	arg1	similarity					729:738	16S rRNA gene sequence similarity	706:738	16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %	706:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	14	20	theme	type	1507:1510	arg1	strain					1512:1517	The type strain	1503:1517	The type strain	1503:1517	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	14	20	theme	type	1507:1510	arg1	THG-YT1T					1556:1563	Camelliibacillus cellulosilyticus THG-YT1T	1522:1563	Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T)	1522:1592	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	11	21	theme	DNA	1231:1233	arg1	G+C content					1235:1245	The DNA G+C content	1227:1245	The DNA G+C content of strain THG-YT1T	1227:1264	The DNA G+C content of strain THG-YT1T was determined to be 53.5 mol%.					
29701574	11	21	theme	DNA	1231:1233	arg1	%					1295:1295	53.5 mol%	1287:1295	53.5 mol%	1287:1295	The DNA G+C content of strain THG-YT1T was determined to be 53.5 mol%.					
29701574	5	22	theme	similarity	729:738	arg1	levels					696:701	levels	696:701	levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %	696:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	10	23	theme	cell-wall	1198:1206	arg1	sugar					1208:1212	The cell-wall sugar	1194:1212	The cell-wall sugar	1194:1212	The cell-wall sugar was glucose.					
29701574	10	23	theme	cell-wall	1198:1206	arg1	glucose					1218:1224	glucose	1218:1224	glucose	1218:1224	The cell-wall sugar was glucose.					
29701574	0	24	theme	cellulosilyticus	17:32	arg1	nov.					39:42	Camelliibacillus cellulosilyticus gen. nov.	0:42	Camelliibacillus cellulosilyticus gen. nov.	0:42	Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	4	25	theme	gene	389:392	arg1	sequences					394:402	16S rRNA gene sequences	380:402	16S rRNA gene sequences	380:402	Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae.					
29701574	5	26	theme	Strain	547:552	arg1	THG-YT1T					554:561	Strain THG-YT1T	547:561	Strain THG-YT1T	547:561	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	7	27	theme	polar	838:842	arg1	phosphatidylethanolamine					856:879	phosphatidylethanolamine	856:879	phosphatidylethanolamine	856:879	The polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified aminolipids and two unidentified glycolipids.					
29701574	7	27	theme	polar	838:842	arg1	lipids					844:849	The polar lipids	834:849	The polar lipids	834:849	The polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified aminolipids and two unidentified glycolipids.					
29701574	5	28	theme	genera	780:785	arg1	members					763:769	members	763:769	members of these genera of less than 95.0 %	763:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	0	29	theme	Camelliibacillus	0:15	arg1	nov.					39:42	Camelliibacillus cellulosilyticus gen. nov.	0:42	Camelliibacillus cellulosilyticus gen. nov.	0:42	Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	4	30	theme	distinct	464:471	arg1	lineage					473:479	a distinct lineage	462:479	a distinct lineage	462:479	Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae.					
29701574	8	31	theme	fatty	992:996	arg1	C18 					1028:1031	C18 	1028:1031	C18 : 3ω7c and anteiso-C17 : 0	1028:1057	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	8	31	theme	fatty	992:996	arg1	acids					998:1002	The major fatty acids	982:1002	The major fatty acids of strain THG-YT1T	982:1021	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	5	32	dep	families	613:620	arg1	Tuberibacillus					658:671	Tuberibacillus	658:671	Tuberibacillus	658:671	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	5	32	dep	families	613:620	arg1	families					613:620	the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu	609:688	the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu	609:688	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	5	32	dep	families	613:620	arg1	Scopulibacillus					641:655	Scopulibacillus	641:655	Scopulibacillus	641:655	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	5	32	dep	families	613:620	arg1	Caenibacillu					677:688	Caenibacillu	677:688	Caenibacillu	677:688	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	8	33	theme	strain	1007:1012	arg1	THG-YT1T					1014:1021	strain THG-YT1T	1007:1021	strain THG-YT1T	1007:1021	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	9	34	theme	diagnostic	1138:1147	arg1	acid					1157:1160	the diagnostic diamino acid	1134:1160	the diagnostic diamino acid	1134:1160	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	34	theme	diagnostic	1138:1147	arg1	type					1088:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	5	35	theme	type	747:750	arg1	species					752:758	the type species	743:758	the type species of members of these genera of less than 95.0 %	743:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	0	36	theme	gen.	34:37	arg1	nov.					39:42	Camelliibacillus cellulosilyticus gen. nov.	0:42	Camelliibacillus cellulosilyticus gen. nov.	0:42	Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	14	37	theme	19471T=CGMCC	1571:1582	arg1	1.16306T					1584:1591	=KACC 19471T=CGMCC 1.16306T	1565:1591	=KACC 19471T=CGMCC 1.16306T	1565:1591	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	14	37	theme	19471T=CGMCC	1571:1582	arg1	THG-YT1T					1556:1563	Camelliibacillus cellulosilyticus THG-YT1T	1522:1563	Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T)	1522:1592	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	2	38	theme	non-motile	189:198	arg1	THG-YT1T					226:233	THG-YT1T	226:233	THG-YT1T	226:233	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	2	38	theme	non-motile	189:198	arg1	bacterium					215:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium	106:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T)	106:234	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	14	39	theme	Camelliibacillus	1522:1537	arg1	strain					1512:1517	The type strain	1503:1517	The type strain	1503:1517	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	14	39	theme	Camelliibacillus	1522:1537	arg1	1.16306T					1584:1591	=KACC 19471T=CGMCC 1.16306T	1565:1591	=KACC 19471T=CGMCC 1.16306T	1565:1591	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	14	39	theme	Camelliibacillus	1522:1537	arg1	THG-YT1T					1556:1563	Camelliibacillus cellulosilyticus THG-YT1T	1522:1563	Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T)	1522:1592	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	3	40	dep	optimum	356:362	arg1	%					367:367	0 %	365:367	0 %	365:367	Growth occurred at 10-40 °C (optimum, 25-30 °C), at pH 6-8 (optimum, 7) and at 0-2 % NaCl (optimum, 0 %).					
29701574	5	41	theme	16S	706:708	arg1	similarity					729:738	16S rRNA gene sequence similarity	706:738	16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %	706:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	4	42	theme	strain	439:444	arg1	THG-YT1T					446:453	strain THG-YT1T	439:453	strain THG-YT1T	439:453	Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae.					
29701574	3	43	dep	pH	317:318	arg1	optimum					325:331	optimum	325:331	optimum	325:331	Growth occurred at 10-40 °C (optimum, 25-30 °C), at pH 6-8 (optimum, 7) and at 0-2 % NaCl (optimum, 0 %).					
29701574	3	43	dep	pH	317:318	arg1	6-8					320:322	6-8	320:322	6-8	320:322	Growth occurred at 10-40 °C (optimum, 25-30 °C), at pH 6-8 (optimum, 7) and at 0-2 % NaCl (optimum, 0 %).					
29701574	2	44	theme	aerobic	180:186	arg1	THG-YT1T					226:233	THG-YT1T	226:233	THG-YT1T	226:233	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	2	44	theme	aerobic	180:186	arg1	bacterium					215:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium	106:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T)	106:234	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	12	45	theme	strain	1332:1337	arg1	THG-YT1T					1339:1346	strain THG-YT1T	1332:1346	strain THG-YT1T	1332:1346	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	5	46	theme	members	763:769	arg1	species					752:758	the type species	743:758	the type species of members of these genera of less than 95.0 %	743:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	3	47	theme	0-2 	344:347	arg1	%					348:348	%	348:348	%	348:348	Growth occurred at 10-40 °C (optimum, 25-30 °C), at pH 6-8 (optimum, 7) and at 0-2 % NaCl (optimum, 0 %).					
29701574	1	48	attach	isolated	87:94	arg1	tea					101:103	tea	101:103	tea	101:103	nov., a cellulose-degrading bacterium isolated from tea.					
29701574	1	48	attach	isolated	87:94	arg2	bacterium					77:85	a cellulose-degrading bacterium	55:85	a cellulose-degrading bacterium isolated from tea	55:103	nov., a cellulose-degrading bacterium isolated from tea.					
29701574	12	49	theme	family	1397:1402	arg1	Bacillaceae					1404:1414	the family Bacillaceae	1393:1414	the family Bacillaceae	1393:1414	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	0	50	dep	sp	45:46	arg1	nov.					39:42	Camelliibacillus cellulosilyticus gen. nov.	0:42	Camelliibacillus cellulosilyticus gen. nov.	0:42	Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	3	51	theme	%	348:348	arg1	optimum					356:362	optimum	356:362	optimum	356:362	Growth occurred at 10-40 °C (optimum, 25-30 °C), at pH 6-8 (optimum, 7) and at 0-2 % NaCl (optimum, 0 %).					
29701574	3	51	theme	%	348:348	arg1	 NaCl					349:353	0-2 % NaCl	344:353	0-2 % NaCl (optimum, 0 %)	344:368	Growth occurred at 10-40 °C (optimum, 25-30 °C), at pH 6-8 (optimum, 7) and at 0-2 % NaCl (optimum, 0 %).					
29701574	4	52	theme	phylogenetic	405:416	arg1	analyses					418:425	phylogenetic analyses	405:425	phylogenetic analyses	405:425	Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae.					
29701574	11	53	theme	strain	1250:1255	arg1	THG-YT1T					1257:1264	strain THG-YT1T	1250:1264	strain THG-YT1T	1250:1264	The DNA G+C content of strain THG-YT1T was determined to be 53.5 mol%.					
29701574	1	54	dep	bacterium	77:85	arg1	nov.					49:52	nov.	49:52	nov.	49:52	nov., a cellulose-degrading bacterium isolated from tea.					
29701574	3	55	dep	optimum	325:331	arg1	7					334:334	7	334:334	7	334:334	Growth occurred at 10-40 °C (optimum, 25-30 °C), at pH 6-8 (optimum, 7) and at 0-2 % NaCl (optimum, 0 %).					
29701574	9	56	theme	glutamic	1179:1186	arg1	acid					1188:1191	glutamic acid	1179:1191	glutamic acid	1179:1191	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	56	theme	glutamic	1179:1186	arg1	type					1088:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	14	57	theme	=KACC	1565:1569	arg1	1.16306T					1584:1591	=KACC 19471T=CGMCC 1.16306T	1565:1591	=KACC 19471T=CGMCC 1.16306T	1565:1591	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	14	57	theme	=KACC	1565:1569	arg1	THG-YT1T					1556:1563	Camelliibacillus cellulosilyticus THG-YT1T	1522:1563	Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T)	1522:1592	The type strain is Camelliibacillus cellulosilyticus THG-YT1T(=KACC 19471T=CGMCC 1.16306T).					
29701574	7	58	theme	unidentified	923:934	arg1	phosphatidylethanolamine					856:879	phosphatidylethanolamine	856:879	phosphatidylethanolamine	856:879	The polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified aminolipids and two unidentified glycolipids.					
29701574	7	58	theme	unidentified	923:934	arg1	aminolipids					936:946	two unidentified aminolipids	919:946	two unidentified aminolipids	919:946	The polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified aminolipids and two unidentified glycolipids.					
29701574	12	59	theme	Camelliibacillus	1436:1451	arg1	nov.					1475:1478	Camelliibacillus cellulosilyticus gen. nov.	1436:1478	the name Camelliibacillus cellulosilyticus gen. nov.	1427:1478	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	7	60	theme	unidentified	956:967	arg1	glycolipids					969:979	two unidentified glycolipids	952:979	two unidentified glycolipids	952:979	The polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified aminolipids and two unidentified glycolipids.					
29701574	7	60	theme	unidentified	956:967	arg1	phosphatidylethanolamine					856:879	phosphatidylethanolamine	856:879	phosphatidylethanolamine	856:879	The polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified aminolipids and two unidentified glycolipids.					
29701574	2	61	theme	rod-shaped	204:213	arg1	THG-YT1T					226:233	THG-YT1T	226:233	THG-YT1T	226:233	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	2	61	theme	rod-shaped	204:213	arg1	bacterium					215:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium	106:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T)	106:234	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	12	62	dep	sp	1481:1482	arg1	name					1431:1434	the name	1427:1434	the name Camelliibacillus cellulosilyticus gen. nov.	1427:1478	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	5	63	with	related	580:586	arg1	levels					696:701	levels	696:701	levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %	696:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	12	64	theme	gen.	1470:1473	arg1	nov.					1475:1478	Camelliibacillus cellulosilyticus gen. nov.	1436:1478	the name Camelliibacillus cellulosilyticus gen. nov.	1427:1478	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	12	65	theme	new	1380:1382	arg1	genus					1384:1388	a new genus	1378:1388	a new genus of the family Bacillaceae	1378:1414	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	9	66	theme	diamino	1149:1155	arg1	acid					1157:1160	the diagnostic diamino acid	1134:1160	the diagnostic diamino acid	1134:1160	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	66	theme	diamino	1149:1155	arg1	type					1088:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	67	theme	cell-wall	1064:1072	arg1	alanine					1167:1173	alanine	1167:1173	alanine	1167:1173	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	67	theme	cell-wall	1064:1072	arg1	acid					1188:1191	glutamic acid	1179:1191	glutamic acid	1179:1191	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	67	theme	cell-wall	1064:1072	arg1	acid					1157:1160	the diagnostic diamino acid	1134:1160	the diagnostic diamino acid	1134:1160	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	67	theme	cell-wall	1064:1072	arg1	type					1088:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	12	68	dep	name	1431:1434	arg1	nov.					1475:1478	Camelliibacillus cellulosilyticus gen. nov.	1436:1478	the name Camelliibacillus cellulosilyticus gen. nov.	1427:1478	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
29701574	4	69	theme	family	527:532	arg1	Bacillaceae					534:544	the family Bacillaceae	523:544	the family Bacillaceae	523:544	Based on 16S rRNA gene sequences, phylogenetic analyses showed that strain THG-YT1T formed a distinct lineage with respect to closely related genera in the family Bacillaceae.					
29701574	5	70	theme	rRNA	710:713	arg1	similarity					729:738	16S rRNA gene sequence similarity	706:738	16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %	706:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	2	71	theme	oxidase-	129:136	arg1	THG-YT1T					226:233	THG-YT1T	226:233	THG-YT1T	226:233	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	2	71	theme	oxidase-	129:136	arg1	bacterium					215:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium	106:223	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T)	106:234	A Gram-stain-positive, oxidase- and catalase-positive, endospore-forming, aerobic, non-motile and rod-shaped bacterium (THG-YT1T) was isolated from green tea.					
29701574	8	72	dep	C18 	1028:1031	arg1	 0					1056:1057	 0	1056:1057	C18 : 3ω7c and anteiso-C17 : 0	1028:1057	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	8	72	dep	C18 	1028:1031	arg1	anteiso-C17 					1043:1054	anteiso-C17 	1043:1054	anteiso-C17 	1043:1054	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	8	72	dep	C18 	1028:1031	arg1	 3ω7c					1033:1037	 3ω7c	1033:1037	 3ω7c	1033:1037	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	9	73	theme	peptidoglycan	1074:1086	arg1	alanine					1167:1173	alanine	1167:1173	alanine	1167:1173	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	73	theme	peptidoglycan	1074:1086	arg1	acid					1188:1191	glutamic acid	1179:1191	glutamic acid	1179:1191	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	73	theme	peptidoglycan	1074:1086	arg1	acid					1157:1160	the diagnostic diamino acid	1134:1160	the diagnostic diamino acid	1134:1160	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	9	73	theme	peptidoglycan	1074:1086	arg1	type					1088:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type	1060:1091	The cell-wall peptidoglycan type was A1γ with meso-diaminopimelic acid as the diagnostic diamino acid plus alanine and glutamic acid.					
29701574	5	74	theme	sequence	720:727	arg1	similarity					729:738	16S rRNA gene sequence similarity	706:738	16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %	706:805	Strain THG-YT1T was most closely related to the genera within the families Pullulanibacillus, Scopulibacillus, Tuberibacillus and Caenibacillu, with levels of 16S rRNA gene sequence similarity to the type species of members of these genera of less than 95.0 %.					
29701574	8	75	theme	THG-YT1T	1014:1021	arg1	C18 					1028:1031	C18 	1028:1031	C18 : 3ω7c and anteiso-C17 : 0	1028:1057	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	8	75	theme	THG-YT1T	1014:1021	arg1	acids					998:1002	The major fatty acids	982:1002	The major fatty acids of strain THG-YT1T	982:1021	The major fatty acids of strain THG-YT1T were C18 : 3ω7c and anteiso-C17 : 0.					
29701574	12	76	theme	cellulosilyticus	1453:1468	arg1	nov.					1475:1478	Camelliibacillus cellulosilyticus gen. nov.	1436:1478	the name Camelliibacillus cellulosilyticus gen. nov.	1427:1478	Based on the data presented here, strain THG-YT1T represents a novel species of a new genus of the family Bacillaceae, for which the name Camelliibacillus cellulosilyticus gen. nov., sp.					
31255519	6	0	theme	glycogen	1112:1119	arg1	contents					1129:1136	glycogen and fat contents	1112:1136	glycogen and fat contents of the liver	1112:1149	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	2	1	theme	weight	539:544	arg1	regulation					546:555	weight regulation	539:555	weight regulation	539:555	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	5	2	theme	insulin	1032:1038	arg1	tests					1050:1054	glucose and insulin tolerance tests	1020:1054	glucose and insulin tolerance tests	1020:1054	Body weight, body composition, energy intake and expenditure were monitored, as well as glucose and insulin tolerance tests.					
31255519	7	3	from	gain	1279:1282	arg1	mice					1295:1298	C57BL/6 mice	1287:1298	C57BL/6 mice	1287:1298	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	1	4	theme	intake	217:222	arg1	imbalance					197:205	an imbalance	194:205	an imbalance of energy intake and expenditure	194:238	OBJECTIVE Although it is widely accepted that obesity results from an imbalance of energy intake and expenditure, the mechanisms underlying this process and effective strategies for prevention and treatment are unclear.					
31255519	0	5	theme	C57BL/6J	66:73	arg1	mice					75:78	C57BL/6J mice	66:78	C57BL/6J mice	66:78	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	6	6	theme	liver	1145:1149	arg1	contents					1129:1136	glycogen and fat contents	1112:1136	glycogen and fat contents of the liver	1112:1149	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	6	6	theme	liver	1145:1149	arg1	Expression					1057:1066	Expression	1057:1066	Expression of sweet taste receptors on the tongue	1057:1105	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	0	7	dep	ingestion	99:107	arg1	liquid					110:115	liquid	110:115	liquid	110:115	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	0	7	dep	ingestion	99:107	arg1	solid					120:124	solid	120:124	solid	120:124	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	0	8	from	Impact	0:5	arg1	adiposity					29:37	adiposity	29:37	adiposity	29:37	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	0	8	from	Impact	0:5	arg1	homeostasis					51:61	glucose homeostasis	43:61	glucose homeostasis	43:61	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	4	9	theme	solid	821:825	arg1	mice					914:917	solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice	821:917	solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice	821:917	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	9	10	theme	sucrose	1463:1469	arg1	contributor					1491:1501	an important contributor	1478:1501	an important contributor to dysregulation of body weight and related metabolic syndromes	1478:1565	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	9	10	theme	sucrose	1463:1469	arg1	consumption					1441:1451	consumption	1441:1451	consumption of liquid sucrose	1441:1469	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	9	11	theme	weight	1528:1533	arg1	dysregulation					1506:1518	dysregulation	1506:1518	dysregulation of body weight and related metabolic syndromes	1506:1565	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	6	12	from	receptors	1083:1091	arg1	tongue					1100:1105	the tongue	1096:1105	the tongue	1096:1105	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	1	13	theme	expenditure	228:238	arg1	imbalance					197:205	an imbalance	194:205	an imbalance of energy intake and expenditure	194:238	OBJECTIVE Although it is widely accepted that obesity results from an imbalance of energy intake and expenditure, the mechanisms underlying this process and effective strategies for prevention and treatment are unclear.					
31255519	4	14	theme	chow	866:869	arg1	part					831:834	part	831:834	part of a standard pelleted rodent chow	831:869	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	8	15	theme	sucrose	1376:1382	arg1	intake					1384:1389	sucrose intake	1376:1389	sucrose intake	1376:1389	Glucose intolerance was positively correlated to body fatness, rather than sucrose intake.					
31255519	7	16	dep	RESULTS	1171:1177	arg1	Consumption					1179:1189	Consumption	1179:1189	RESULTS Consumption of sucrose-sweetened water	1171:1216	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	7	17	theme	C57BL/6	1287:1293	arg1	mice					1295:1298	C57BL/6 mice	1287:1298	C57BL/6 mice	1287:1298	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	4	18	theme	liquid	876:881	arg1	mice					914:917	solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice	821:917	solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice	821:917	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	4	19	theme	METHODS	780:786	arg1	sucrose					796:802	METHODS Dietary sucrose	780:802	METHODS Dietary sucrose	780:802	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	7	20	theme	fat	1275:1277	arg1	gain					1279:1282	body fat gain	1270:1282	body fat gain in C57BL/6 mice	1270:1298	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	7	21	theme	sucrose-sweetened	1194:1210	arg1	water					1212:1216	sucrose-sweetened water	1194:1216	sucrose-sweetened water	1194:1216	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	0	22	from	adiposity	29:37	arg1	mice					75:78	C57BL/6J mice	66:78	C57BL/6J mice	66:78	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	3	23	theme	glucose	759:765	arg1	homeostasis					767:777	glucose homeostasis	759:777	glucose homeostasis	759:777	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	3	24	contain	had	707:709	arg2	impacts					721:727	different impacts	711:727	different impacts	711:727	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	3	24	contain	had	707:709	arg1	mode					671:674	the mode	667:674	the mode of ingestion (solid or liquid)	667:705	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	2	25	theme	excess	373:378	arg1	consumption					380:390	excess consumption	373:390	excess consumption of sugar	373:399	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	4	26	theme	drinking	887:894	arg1	water					896:900	drinking water	887:900	drinking water	887:900	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	9	27	theme	related	1539:1545	arg1	syndromes					1557:1565	related metabolic syndromes	1539:1565	related metabolic syndromes	1539:1565	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	6	28	from	Expression	1057:1066	arg1	tongue					1100:1105	the tongue	1096:1105	the tongue	1096:1105	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	4	29	theme	rodent	859:864	arg1	chow					866:869	a standard pelleted rodent chow	839:869	a standard pelleted rodent chow	839:869	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	4	30	theme	Dietary	788:794	arg1	sucrose					796:802	METHODS Dietary sucrose	780:802	METHODS Dietary sucrose	780:802	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	7	31	theme	body	1270:1273	arg1	gain					1279:1282	body fat gain	1270:1282	body fat gain in C57BL/6 mice	1270:1298	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	2	32	theme	important	413:421	arg1	role					423:426	an important role	410:426	an important role	410:426	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	9	33	theme	important	1481:1489	arg1	consumption					1441:1451	consumption	1441:1451	consumption of liquid sucrose	1441:1469	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	9	33	theme	important	1481:1489	arg1	contributor					1491:1501	an important contributor	1478:1501	an important contributor to dysregulation of body weight and related metabolic syndromes	1478:1565	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	4	34	theme	standard	841:848	arg1	chow					866:869	a standard pelleted rodent chow	839:869	a standard pelleted rodent chow	839:869	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	9	35	theme	metabolic	1547:1555	arg1	syndromes					1557:1565	related metabolic syndromes	1539:1565	related metabolic syndromes	1539:1565	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	0	36	theme	dietary	10:16	arg1	sucrose					18:24	dietary sucrose	10:24	dietary sucrose	10:24	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	3	37	theme	ingestion	679:687	arg1	mode					671:674	the mode	667:674	the mode of ingestion (solid or liquid)	667:705	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	0	38	theme	ingestion	99:107	arg1	mode					91:94	mode	91:94	mode of ingestion: liquid or solid	91:124	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	4	39	theme	pelleted	850:857	arg1	chow					866:869	a standard pelleted rodent chow	839:869	a standard pelleted rodent chow	839:869	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	7	40	theme	equivalent	1227:1236	arg1	levels					1238:1243	equivalent levels	1227:1243	equivalent levels of solid sucrose	1227:1260	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	4	41	dep	solid	821:825	arg1	part					831:834	part	831:834	part of a standard pelleted rodent chow	831:869	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	4	41	dep	solid	821:825	arg1	water					896:900	drinking water	887:900	drinking water	887:900	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	2	42	theme	calories	490:497	arg1	calories					490:497	calories	490:497	calories as sucrose in the diet	490:520	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	2	42	theme	calories	490:497	arg1	%					485:485	consuming up to 30%	467:485	consuming up to 30% of calories as sucrose in the diet	467:520	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	3	43	theme	sugar-sweetened	589:603	arg1	beverages					605:613	sugar-sweetened beverages	589:613	sugar-sweetened beverages	589:613	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	7	44	theme	solid	1248:1252	arg1	sucrose					1254:1260	solid sucrose	1248:1260	solid sucrose	1248:1260	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	2	45	contain	had	522:524	arg1	calories					490:497	calories	490:497	calories as sucrose in the diet	490:520	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	2	45	contain	had	522:524	arg2	impact					529:534	no impact	526:534	no impact	526:534	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	2	45	contain	had	522:524	arg1	%					485:485	consuming up to 30%	467:485	consuming up to 30% of calories as sucrose in the diet	467:520	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	2	46	theme	sugar	395:399	arg1	consumption					380:390	excess consumption	373:390	excess consumption of sugar	373:399	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	5	47	theme	glucose	1020:1026	arg1	tests					1050:1054	glucose and insulin tolerance tests	1020:1054	glucose and insulin tolerance tests	1020:1054	Body weight, body composition, energy intake and expenditure were monitored, as well as glucose and insulin tolerance tests.					
31255519	2	48	dep	30	483:484	arg1	to					480:481	to	480:481	to	480:481	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	2	48	dep	30	483:484	arg1	up					477:478	up	477:478	up	477:478	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	5	49	theme	energy	963:968	arg1	intake					970:975	energy intake	963:975	energy intake	963:975	Body weight, body composition, energy intake and expenditure were monitored, as well as glucose and insulin tolerance tests.					
31255519	0	50	theme	sucrose	18:24	arg1	Impact					0:5	Impact	0:5	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice	0:78	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	0	51	theme	glucose	43:49	arg1	homeostasis					51:61	glucose homeostasis	43:61	glucose homeostasis	43:61	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	3	52	theme	different	711:719	arg1	impacts					721:727	different impacts	711:727	different impacts	711:727	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	9	53	theme	liquid	1456:1461	arg1	sucrose					1463:1469	liquid sucrose	1456:1469	liquid sucrose	1456:1469	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	0	54	from	homeostasis	51:61	arg1	mice					75:78	C57BL/6J mice	66:78	C57BL/6J mice	66:78	Impact of dietary sucrose on adiposity and glucose homeostasis in C57BL/6J mice depends on mode of ingestion: liquid or solid.					
31255519	8	55	theme	body	1350:1353	arg1	fatness					1355:1361	body fatness	1350:1361	body fatness	1350:1361	Glucose intolerance was positively correlated to body fatness, rather than sucrose intake.					
31255519	4	56	theme	C57BL/6	906:912	arg1	mice					914:917	solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice	821:917	solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice	821:917	METHODS Dietary sucrose was delivered in solid (as part of a standard pelleted rodent chow) and liquid (in drinking water) to C57BL/6 mice for 8 weeks.					
31255519	1	57	theme	effective	284:292	arg1	strategies					294:303	effective strategies	284:303	effective strategies for prevention and treatment	284:332	OBJECTIVE Although it is widely accepted that obesity results from an imbalance of energy intake and expenditure, the mechanisms underlying this process and effective strategies for prevention and treatment are unclear.					
31255519	9	58	theme	syndromes	1557:1565	arg1	dysregulation					1506:1518	dysregulation	1506:1518	dysregulation of body weight and related metabolic syndromes	1506:1565	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	5	59	theme	Body	932:935	arg1	weight					937:942	Body weight	932:942	Body weight	932:942	Body weight, body composition, energy intake and expenditure were monitored, as well as glucose and insulin tolerance tests.					
31255519	6	60	theme	fat	1125:1127	arg1	contents					1129:1136	glycogen and fat contents	1112:1136	glycogen and fat contents of the liver	1112:1149	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	9	61	theme	body	1523:1526	arg1	weight					1528:1533	body weight	1523:1533	body weight	1523:1533	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	6	62	from	tongue	1100:1105	arg1	contents					1129:1136	glycogen and fat contents	1112:1136	glycogen and fat contents of the liver	1112:1149	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	6	62	from	tongue	1100:1105	arg1	Expression					1057:1066	Expression	1057:1066	Expression of sweet taste receptors on the tongue	1057:1105	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	5	63	theme	tolerance	1040:1048	arg1	tests					1050:1054	glucose and insulin tolerance tests	1020:1054	glucose and insulin tolerance tests	1020:1054	Body weight, body composition, energy intake and expenditure were monitored, as well as glucose and insulin tolerance tests.					
31255519	7	64	theme	sucrose	1254:1260	arg1	levels					1238:1243	equivalent levels	1227:1243	equivalent levels of solid sucrose	1227:1260	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	9	65	dep	CONCLUSIONS	1392:1402	arg1	support					1413:1419	support	1413:1419	support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes	1413:1565	CONCLUSIONS Our data support the suggestion that consumption of liquid sucrose may be an important contributor to dysregulation of body weight and related metabolic syndromes.					
31255519	3	66	theme	body	732:735	arg1	weight					737:742	body weight	732:742	body weight regulation	732:753	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	8	67	theme	Glucose	1301:1307	arg1	intolerance					1309:1319	Glucose intolerance	1301:1319	Glucose intolerance	1301:1319	Glucose intolerance was positively correlated to body fatness, rather than sucrose intake.					
31255519	3	68	theme	beverages	605:613	arg1	consumption					574:584	consumption	574:584	consumption of sugar-sweetened beverages	574:613	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	6	69	theme	receptors	1083:1091	arg1	contents					1129:1136	glycogen and fat contents	1112:1136	glycogen and fat contents of the liver	1112:1149	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	6	69	theme	receptors	1083:1091	arg1	Expression					1057:1066	Expression	1057:1066	Expression of sweet taste receptors on the tongue	1057:1105	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	3	70	theme	weight	737:742	arg1	regulation					744:753	body weight regulation	732:753	body weight regulation	732:753	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	5	71	theme	body	945:948	arg1	composition					950:960	body composition	945:960	body composition	945:960	Body weight, body composition, energy intake and expenditure were monitored, as well as glucose and insulin tolerance tests.					
31255519	6	72	theme	taste	1077:1081	arg1	receptors					1083:1091	sweet taste receptors	1071:1091	sweet taste receptors on the tongue	1071:1105	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	2	73	theme	Growing	347:353	arg1	evidence					355:362	Growing evidence	347:362	Growing evidence	347:362	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	7	74	theme	water	1212:1216	arg1	Consumption					1179:1189	Consumption	1179:1189	RESULTS Consumption of sucrose-sweetened water	1171:1216	RESULTS Consumption of sucrose-sweetened water, but not equivalent levels of solid sucrose, led to body fat gain in C57BL/6 mice.					
31255519	2	75	from	sucrose	502:508	arg1	diet					517:520	the diet	513:520	the diet	513:520	Growing evidence suggests excess consumption of sugar may play an important role, yet we showed previously in mice that consuming up to 30% of calories as sucrose in the diet had no impact on weight regulation.					
31255519	6	76	from	contents	1129:1136	arg1	tongue					1100:1105	the tongue	1096:1105	the tongue	1096:1105	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	3	77	dep	ingestion	679:687	arg1	solid					690:694	solid	690:694	solid	690:694	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	3	77	dep	ingestion	679:687	arg1	liquid					699:704	liquid	699:704	liquid	699:704	Since in humans consumption of sugar-sweetened beverages has been widely implicated, we investigated whether the mode of ingestion (solid or liquid) had different impacts on body weight regulation and glucose homeostasis.					
31255519	6	78	theme	sweet	1071:1075	arg1	receptors					1083:1091	sweet taste receptors	1071:1091	sweet taste receptors on the tongue	1071:1105	Expression of sweet taste receptors on the tongue, and glycogen and fat contents of the liver were also measured.					
31255519	1	79	theme	energy	210:215	arg1	intake					217:222	energy intake	210:222	energy intake	210:222	OBJECTIVE Although it is widely accepted that obesity results from an imbalance of energy intake and expenditure, the mechanisms underlying this process and effective strategies for prevention and treatment are unclear.					
32070558	0	0	theme	electrokinetic	85:98	arg1	LVSS-PS-CDEKC					116:128	LVSS-PS-CDEKC	116:128	LVSS-PS-CDEKC	116:128	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	0	0	theme	electrokinetic	85:98	arg1	chromatography					100:113	cyclodextrin electrokinetic chromatography	72:113	cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC)	72:129	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	6	1	theme	electrophoretic	1265:1279	arg1	separation					1281:1290	The electrophoretic separation	1261:1290	The electrophoretic separation	1261:1290	The electrophoretic separation was performed in a 2 mM α-cyclodextrin and 25 mM tetraborate system (pH = 9.3) with an applied voltage of 25 kV.					
32070558	1	2	with	stacking	236:243	arg1	PS					277:278	PS	277:278	PS	277:278	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	2	with	stacking	236:243	arg1	switching					266:274	polarity switching	257:274	polarity switching (PS)	257:279	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	4	3	theme	analytes	938:945	arg1	limits					928:933	The detection limits	914:933	The detection limits of analytes for the elaborated LVSS-PS-CDEKC method	914:985	The detection limits of analytes for the elaborated LVSS-PS-CDEKC method were found to be in 0.8-5 ng mL-1 range, which were around 500 times lower than normal CDEKC without preconcentration technique.					
32070558	0	4	from	pharmaceuticals	182:196	arg1	determination					139:151	the determination	135:151	the determination of selected preservatives in pharmaceuticals	135:196	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	0	5	theme	cyclodextrin	72:83	arg1	LVSS-PS-CDEKC					116:128	LVSS-PS-CDEKC	116:128	LVSS-PS-CDEKC	116:128	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	0	5	theme	cyclodextrin	72:83	arg1	chromatography					100:113	cyclodextrin electrokinetic chromatography	72:113	cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC)	72:129	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	4	6	theme	elaborated	955:964	arg1	method					980:985	the elaborated LVSS-PS-CDEKC method	951:985	the elaborated LVSS-PS-CDEKC method	951:985	The detection limits of analytes for the elaborated LVSS-PS-CDEKC method were found to be in 0.8-5 ng mL-1 range, which were around 500 times lower than normal CDEKC without preconcentration technique.					
32070558	7	7	from	preservatives	1502:1514	arg1	control					1529:1535	a quality control	1519:1535	a quality control of pharmaceuticals	1519:1554	The established method was validated and confirmed to be applicable for the determination of the preservatives in a quality control of pharmaceuticals.					
32070558	1	8	theme	benzoic	551:557	arg1	BA					565:566	BA	565:566	BA	565:566	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	8	theme	benzoic	551:557	arg1	acid					559:562	benzoic acid	551:562	benzoic acid (BA)	551:567	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	8	theme	benzoic	551:557	arg1	stacking					236:243	a large volume sample stacking	214:243	a large volume sample stacking (LVSS) with polarity switching (PS)	214:279	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	9	theme	polarity	257:264	arg1	PS					277:278	PS	277:278	PS	277:278	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	9	theme	polarity	257:264	arg1	switching					266:274	polarity switching	257:274	polarity switching (PS)	257:279	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	7	10	theme	preservatives	1502:1514	arg1	determination					1481:1493	the determination	1477:1493	the determination of the preservatives in a quality control of pharmaceuticals	1477:1554	The established method was validated and confirmed to be applicable for the determination of the preservatives in a quality control of pharmaceuticals.					
32070558	5	11	from	11 min	1167:1172	arg1	capillary					1202:1210	an uncoated fused-silica capillary	1177:1210	an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length	1177:1258	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	4	12	theme	0.8-5 ng mL-1	1007:1019	arg1	range					1021:1025	0.8-5 ng mL-1 range	1007:1025	0.8-5 ng mL-1 range	1007:1025	The detection limits of analytes for the elaborated LVSS-PS-CDEKC method were found to be in 0.8-5 ng mL-1 range, which were around 500 times lower than normal CDEKC without preconcentration technique.					
32070558	5	13	theme	diameter	1230:1237	arg1	capillary					1202:1210	an uncoated fused-silica capillary	1177:1210	an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length	1177:1258	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	2	14	theme	modifier	751:758	arg1	concentration					760:772	organic modifier concentration	743:772	organic modifier concentration	743:772	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	15	theme	parameters	647:656	arg1	effects					623:629	The effects	619:629	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration	619:772	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	6	16	theme	25 mM	1335:1339	arg1	system					1353:1358	a 2 mM α-cyclodextrin and 25 mM tetraborate system	1309:1358	system	1353:1358	The electrophoretic separation was performed in a 2 mM α-cyclodextrin and 25 mM tetraborate system (pH = 9.3) with an applied voltage of 25 kV.					
32070558	2	17	theme	typical	639:645	arg1	composition					698:708	composition	698:708	composition	698:708	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	17	theme	typical	639:645	arg1	volume					673:678	sample volume	666:678	sample volume	666:678	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	17	theme	typical	639:645	arg1	pH					714:715	pH	714:715	pH	714:715	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	17	theme	typical	639:645	arg1	parameters					647:656	some typical parameters	634:656	some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration	634:772	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	17	theme	typical	639:645	arg1	concentration					760:772	organic modifier concentration	743:772	organic modifier concentration	743:772	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	17	theme	typical	639:645	arg1	voltage					689:695	applied voltage	681:695	applied voltage	681:695	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	4	18	theme	detection	918:926	arg1	limits					928:933	The detection limits	914:933	The detection limits of analytes for the elaborated LVSS-PS-CDEKC method	914:985	The detection limits of analytes for the elaborated LVSS-PS-CDEKC method were found to be in 0.8-5 ng mL-1 range, which were around 500 times lower than normal CDEKC without preconcentration technique.					
32070558	6	19	theme	applied	1379:1385	arg1	voltage					1387:1393	an applied voltage	1376:1393	an applied voltage of 25 kV	1376:1402	The electrophoretic separation was performed in a 2 mM α-cyclodextrin and 25 mM tetraborate system (pH = 9.3) with an applied voltage of 25 kV.					
32070558	7	20	from	control	1529:1535	arg1	determination					1481:1493	the determination	1477:1493	the determination of the preservatives in a quality control of pharmaceuticals	1477:1554	The established method was validated and confirmed to be applicable for the determination of the preservatives in a quality control of pharmaceuticals.					
32070558	4	21	theme	preconcentration	1088:1103	arg1	technique					1105:1113	preconcentration technique	1088:1113	preconcentration technique	1088:1113	The detection limits of analytes for the elaborated LVSS-PS-CDEKC method were found to be in 0.8-5 ng mL-1 range, which were around 500 times lower than normal CDEKC without preconcentration technique.					
32070558	4	22	theme	normal	1067:1072	arg1	CDEKC					1074:1078	normal CDEKC	1067:1078	normal CDEKC without preconcentration technique	1067:1113	The detection limits of analytes for the elaborated LVSS-PS-CDEKC method were found to be in 0.8-5 ng mL-1 range, which were around 500 times lower than normal CDEKC without preconcentration technique.					
32070558	0	23	theme	volume	21:26	arg1	sample					28:33	large volume sample	15:33	large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals	15:196	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	0	24	theme	large	15:19	arg1	sample					28:33	large volume sample	15:33	large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals	15:196	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	4	25	theme	LVSS-PS-CDEKC	966:978	arg1	method					980:985	the elaborated LVSS-PS-CDEKC method	951:985	the elaborated LVSS-PS-CDEKC method	951:985	The detection limits of analytes for the elaborated LVSS-PS-CDEKC method were found to be in 0.8-5 ng mL-1 range, which were around 500 times lower than normal CDEKC without preconcentration technique.					
32070558	1	26	theme	simultaneous	370:381	arg1	separation					383:392	the simultaneous separation	366:392	the simultaneous separation	366:392	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	5	27	theme	internal	1221:1228	arg1	I.D					1240:1242	I.D	1240:1242	I.D	1240:1242	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	5	27	theme	internal	1221:1228	arg1	diameter					1230:1237	75 μm internal diameter	1215:1237	75 μm internal diameter (I.D)	1215:1243	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	2	28	theme	sample	666:671	arg1	volume					673:678	sample volume	666:678	sample volume	666:678	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	6	29	theme	2 mM	1311:1314	arg1	pH = 9.3					1361:1368	pH = 9.3	1361:1368	pH = 9.3	1361:1368	The electrophoretic separation was performed in a 2 mM α-cyclodextrin and 25 mM tetraborate system (pH = 9.3) with an applied voltage of 25 kV.					
32070558	6	29	theme	2 mM	1311:1314	arg1	α-cyclodextrin					1316:1329	a 2 mM α-cyclodextrin and 25 mM tetraborate system	1309:1358	α-cyclodextrin	1316:1329	The electrophoretic separation was performed in a 2 mM α-cyclodextrin and 25 mM tetraborate system (pH = 9.3) with an applied voltage of 25 kV.					
32070558	7	30	theme	quality	1521:1527	arg1	control					1529:1535	a quality control	1519:1535	a quality control of pharmaceuticals	1519:1554	The established method was validated and confirmed to be applicable for the determination of the preservatives in a quality control of pharmaceuticals.					
32070558	7	31	theme	pharmaceuticals	1540:1554	arg1	control					1529:1535	a quality control	1519:1535	a quality control of pharmaceuticals	1519:1554	The established method was validated and confirmed to be applicable for the determination of the preservatives in a quality control of pharmaceuticals.					
32070558	6	32	theme	25 kV	1398:1402	arg1	voltage					1387:1393	an applied voltage	1376:1393	an applied voltage of 25 kV	1376:1402	The electrophoretic separation was performed in a 2 mM α-cyclodextrin and 25 mM tetraborate system (pH = 9.3) with an applied voltage of 25 kV.					
32070558	6	33	theme	tetraborate	1341:1351	arg1	system					1353:1358	a 2 mM α-cyclodextrin and 25 mM tetraborate system	1309:1358	system	1353:1358	The electrophoretic separation was performed in a 2 mM α-cyclodextrin and 25 mM tetraborate system (pH = 9.3) with an applied voltage of 25 kV.					
32070558	5	34	theme	fused-silica	1189:1200	arg1	capillary					1202:1210	an uncoated fused-silica capillary	1177:1210	an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length	1177:1258	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	1	35	theme	large	216:220	arg1	acid					540:543	sorbic acid	533:543	sorbic acid (SA)	533:548	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	35	theme	large	216:220	arg1	acid					559:562	benzoic acid	551:562	benzoic acid (BA)	551:567	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	35	theme	large	216:220	arg1	stacking					236:243	a large volume sample stacking	214:243	a large volume sample stacking (LVSS) with polarity switching (PS)	214:279	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	35	theme	large	216:220	arg1	isobutylparaben					510:524	isobutylparaben	510:524	isobutylparaben (IBP)	510:530	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	35	theme	large	216:220	arg1	butylparaben					491:502	butylparaben	491:502	butylparaben (BP)	491:507	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	35	theme	large	216:220	arg1	MP					447:448	MP	447:448	MP	447:448	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	35	theme	large	216:220	arg1	LVSS					246:249	LVSS	246:249	LVSS	246:249	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	35	theme	large	216:220	arg1	propylparaben					471:483	propylparaben	471:483	propylparaben (PP)	471:488	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	35	theme	large	216:220	arg1	ethylparaben					452:463	ethylparaben	452:463	ethylparaben (EP)	452:468	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	35	theme	large	216:220	arg1	acid					587:590	p-hydroxybenzoic acid	570:590	p-hydroxybenzoic acid (PHBA) in pharmaceuticals	570:616	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	0	36	theme	sample	28:33	arg1	Combination					0:10	Combination	0:10	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.	0:197	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	1	37	theme	cyclodextrin	285:296	arg1	method					336:341	cyclodextrin electrokinetic chromatography (CDEKC) method	285:341	cyclodextrin electrokinetic chromatography (CDEKC) method	285:341	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	38	theme	p-hydroxybenzoic	570:585	arg1	PHBA					593:596	PHBA	593:596	PHBA	593:596	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	38	theme	p-hydroxybenzoic	570:585	arg1	acid					587:590	p-hydroxybenzoic acid	570:590	p-hydroxybenzoic acid (PHBA) in pharmaceuticals	570:616	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	38	theme	p-hydroxybenzoic	570:585	arg1	stacking					236:243	a large volume sample stacking	214:243	a large volume sample stacking (LVSS) with polarity switching (PS)	214:279	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	5	39	theme	75 μm	1215:1219	arg1	I.D					1240:1242	I.D	1240:1242	I.D	1240:1242	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	5	39	theme	75 μm	1215:1219	arg1	diameter					1230:1237	75 μm internal diameter	1215:1237	75 μm internal diameter (I.D)	1215:1243	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	1	40	from	acid	587:590	arg1	pharmaceuticals					602:616	pharmaceuticals	602:616	pharmaceuticals	602:616	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	acid					540:543	sorbic acid	533:543	sorbic acid (SA)	533:548	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	acid					559:562	benzoic acid	551:562	benzoic acid (BA)	551:567	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	stacking					236:243	a large volume sample stacking	214:243	a large volume sample stacking (LVSS) with polarity switching (PS)	214:279	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	isobutylparaben					510:524	isobutylparaben	510:524	isobutylparaben (IBP)	510:530	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	butylparaben					491:502	butylparaben	491:502	butylparaben (BP)	491:507	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	MP					447:448	MP	447:448	MP	447:448	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	LVSS					246:249	LVSS	246:249	LVSS	246:249	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	propylparaben					471:483	propylparaben	471:483	propylparaben (PP)	471:488	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	ethylparaben					452:463	ethylparaben	452:463	ethylparaben (EP)	452:468	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	41	theme	volume	222:227	arg1	acid					587:590	p-hydroxybenzoic acid	570:590	p-hydroxybenzoic acid (PHBA) in pharmaceuticals	570:616	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	0	42	theme	polarity	49:56	arg1	switching					58:66	polarity switching	49:66	polarity switching	49:66	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	1	43	theme	electrokinetic	298:311	arg1	method					336:341	cyclodextrin electrokinetic chromatography (CDEKC) method	285:341	cyclodextrin electrokinetic chromatography (CDEKC) method	285:341	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	0	44	theme	preservatives	165:177	arg1	determination					139:151	the determination	135:151	the determination of selected preservatives in pharmaceuticals	135:196	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	1	45	dep	stacking	236:243	arg1	developed					352:360	developed	352:360	has been developed for the simultaneous separation and determination of 8 preservatives	343:429	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	45	dep	stacking	236:243	arg1	study					207:211	this study	202:211	this study	202:211	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	46	theme	chromatography	313:326	arg1	method					336:341	cyclodextrin electrokinetic chromatography (CDEKC) method	285:341	cyclodextrin electrokinetic chromatography (CDEKC) method	285:341	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	0	47	theme	selected	156:163	arg1	preservatives					165:177	selected preservatives	156:177	selected preservatives in pharmaceuticals	156:196	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	3	48	theme	type	827:830	arg1	impact					817:822	the impact	813:822	the impact of type and concentration of cyclodextrin as electrolyte modifiers	813:889	Moreover, the impact of type and concentration of cyclodextrin as electrolyte modifiers was also investigated.					
32070558	7	49	theme	established	1409:1419	arg1	method					1421:1426	The established method	1405:1426	The established method	1405:1426	The established method was validated and confirmed to be applicable for the determination of the preservatives in a quality control of pharmaceuticals.					
32070558	7	49	theme	established	1409:1419	arg1	applicable					1462:1471	applicable	1462:1471	applicable	1462:1471	The established method was validated and confirmed to be applicable for the determination of the preservatives in a quality control of pharmaceuticals.					
32070558	1	50	theme	sorbic	533:538	arg1	acid					540:543	sorbic acid	533:543	sorbic acid (SA)	533:548	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	50	theme	sorbic	533:538	arg1	stacking					236:243	a large volume sample stacking	214:243	a large volume sample stacking (LVSS) with polarity switching (PS)	214:279	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	50	theme	sorbic	533:538	arg1	SA					546:547	SA	546:547	SA	546:547	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	acid					540:543	sorbic acid	533:543	sorbic acid (SA)	533:548	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	acid					559:562	benzoic acid	551:562	benzoic acid (BA)	551:567	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	stacking					236:243	a large volume sample stacking	214:243	a large volume sample stacking (LVSS) with polarity switching (PS)	214:279	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	isobutylparaben					510:524	isobutylparaben	510:524	isobutylparaben (IBP)	510:530	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	butylparaben					491:502	butylparaben	491:502	butylparaben (BP)	491:507	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	MP					447:448	MP	447:448	MP	447:448	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	LVSS					246:249	LVSS	246:249	LVSS	246:249	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	propylparaben					471:483	propylparaben	471:483	propylparaben (PP)	471:488	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	ethylparaben					452:463	ethylparaben	452:463	ethylparaben (EP)	452:468	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	51	theme	sample	229:234	arg1	acid					587:590	p-hydroxybenzoic acid	570:590	p-hydroxybenzoic acid (PHBA) in pharmaceuticals	570:616	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	7	52	from	determination	1481:1493	arg1	control					1529:1535	a quality control	1519:1535	a quality control of pharmaceuticals	1519:1554	The established method was validated and confirmed to be applicable for the determination of the preservatives in a quality control of pharmaceuticals.					
32070558	1	53	theme	preservatives	417:429	arg1	determination					398:410	determination	398:410	determination of 8 preservatives	398:429	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	53	theme	preservatives	417:429	arg1	separation					383:392	the simultaneous separation	366:392	the simultaneous separation	366:392	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	3	54	theme	concentration	836:848	arg1	impact					817:822	the impact	813:822	the impact of type and concentration of cyclodextrin as electrolyte modifiers	813:889	Moreover, the impact of type and concentration of cyclodextrin as electrolyte modifiers was also investigated.					
32070558	3	55	theme	electrolyte	869:879	arg1	modifiers					881:889	electrolyte modifiers	869:889	electrolyte modifiers	869:889	Moreover, the impact of type and concentration of cyclodextrin as electrolyte modifiers was also investigated.					
32070558	5	56	theme	uncoated	1180:1187	arg1	capillary					1202:1210	an uncoated fused-silica capillary	1177:1210	an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length	1177:1258	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	0	57	from	determination	139:151	arg1	pharmaceuticals					182:196	pharmaceuticals	182:196	pharmaceuticals	182:196	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	5	58	theme	length	1253:1258	arg1	capillary					1202:1210	an uncoated fused-silica capillary	1177:1210	an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length	1177:1258	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	5	59	theme	50 cm	1247:1251	arg1	length					1253:1258	50 cm length	1247:1258	50 cm length	1247:1258	All analytes were completely resolved in less than 11 min in an uncoated fused-silica capillary of 75 μm internal diameter (I.D) x 50 cm length.					
32070558	2	60	theme	applied	681:687	arg1	voltage					689:695	applied voltage	681:695	applied voltage	681:695	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	61	theme	buffer	732:737	arg1	concentration					760:772	organic modifier concentration	743:772	organic modifier concentration	743:772	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	61	theme	buffer	732:737	arg1	composition					698:708	composition	698:708	composition	698:708	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	61	theme	buffer	732:737	arg1	volume					673:678	sample volume	666:678	sample volume	666:678	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	61	theme	buffer	732:737	arg1	voltage					689:695	applied voltage	681:695	applied voltage	681:695	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	2	61	theme	buffer	732:737	arg1	pH					714:715	pH	714:715	pH	714:715	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	0	62	from	preservatives	165:177	arg1	pharmaceuticals					182:196	pharmaceuticals	182:196	pharmaceuticals	182:196	Combination of large volume sample stacking with polarity switching and cyclodextrin electrokinetic chromatography (LVSS-PS-CDEKC) for the determination of selected preservatives in pharmaceuticals.					
32070558	1	63	theme	CDEKC	329:333	arg1	method					336:341	cyclodextrin electrokinetic chromatography (CDEKC) method	285:341	cyclodextrin electrokinetic chromatography (CDEKC) method	285:341	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	3	64	theme	cyclodextrin	853:864	arg1	concentration					836:848	concentration	836:848	concentration	836:848	Moreover, the impact of type and concentration of cyclodextrin as electrolyte modifiers was also investigated.					
32070558	3	64	theme	cyclodextrin	853:864	arg1	type					827:830	type	827:830	type	827:830	Moreover, the impact of type and concentration of cyclodextrin as electrolyte modifiers was also investigated.					
32070558	2	65	theme	organic	743:749	arg1	concentration					760:772	organic modifier concentration	743:772	organic modifier concentration	743:772	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
32070558	1	66	with	method	336:341	arg1	PS					277:278	PS	277:278	PS	277:278	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	1	66	with	method	336:341	arg1	switching					266:274	polarity switching	257:274	polarity switching (PS)	257:279	In this study, a large volume sample stacking (LVSS) with polarity switching (PS) and cyclodextrin electrokinetic chromatography (CDEKC) method has been developed for the simultaneous separation and determination of 8 preservatives: methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP), isobutylparaben (IBP), sorbic acid (SA), benzoic acid (BA), p-hydroxybenzoic acid (PHBA) in pharmaceuticals.					
32070558	2	67	theme	running	724:730	arg1	buffer					732:737	the running buffer	720:737	the running buffer	720:737	The effects of some typical parameters such as sample volume, applied voltage, composition and pH of the running buffer and organic modifier concentration were examined and optimized.					
30804449	4	0	theme	Paneth	915:920	arg1	secretion					927:935	Paneth cell secretion	915:935	Paneth cell secretion	915:935	Here, we report that microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen provides an ex vivo system for studying Paneth cell secretion in real-time.					
30804449	3	1	theme	epithelial	531:540	arg1	cells					542:546	small intestinal epithelial cells	514:546	small intestinal epithelial cells	514:546	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	2	2	theme	secretory	355:363	arg1	responses					365:373	Paneth cell secretory responses	343:373	Paneth cell secretory responses	343:373	However, Paneth cell secretory responses remain debatable and the mechanisms that regulate the secretion are not well understood.					
30804449	1	3	theme	enteric	255:261	arg1	pathogens					263:271	enteric pathogens	255:271	enteric pathogens	255:271	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	8	4	theme	secretory	1477:1485	arg1	responses					1487:1495	apically-induced Paneth cell secretory responses	1448:1495	apically-induced Paneth cell secretory responses in regulating the intestinal environment	1448:1536	These findings provide new insight for apically-induced Paneth cell secretory responses in regulating the intestinal environment.					
30804449	3	5	theme	epithelial	593:602	arg1	transport					633:641	ion transport	629:641	ion transport	629:641	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	3	5	theme	epithelial	593:602	arg1	functions					609:617	intestinal epithelial cell functions	582:617	intestinal epithelial cell functions including ion transport	582:641	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	8	6	theme	new	1432:1434	arg1	insight					1436:1442	new insight	1432:1442	new insight for apically-induced Paneth cell secretory responses in regulating the intestinal environment	1432:1536	These findings provide new insight for apically-induced Paneth cell secretory responses in regulating the intestinal environment.					
30804449	4	7	theme	cell	922:925	arg1	secretion					927:935	Paneth cell secretion	915:935	Paneth cell secretion	915:935	Here, we report that microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen provides an ex vivo system for studying Paneth cell secretion in real-time.					
30804449	4	8	theme	bacteria	814:821	arg1	microinjection					796:809	microinjection	796:809	microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen	796:873	Here, we report that microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen provides an ex vivo system for studying Paneth cell secretion in real-time.					
30804449	5	9	theme	Paneth	973:978	arg1	cells					980:984	Paneth cells	973:984	Paneth cells	973:984	The results show that Paneth cells released granules immediately when the apical surfaces of enteroid epithelial cells were exposed to LPS or live bacteria by microinjection.					
30804449	3	10	theme	small	514:518	arg1	cells					542:546	small intestinal epithelial cells	514:546	small intestinal epithelial cells	514:546	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	5	11	theme	apical	1025:1030	arg1	surfaces					1032:1039	the apical surfaces	1021:1039	the apical surfaces of enteroid epithelial cells	1021:1068	The results show that Paneth cells released granules immediately when the apical surfaces of enteroid epithelial cells were exposed to LPS or live bacteria by microinjection.					
30804449	4	12	theme	ex	887:888	arg1	system					895:900	an ex vivo system	884:900	an ex vivo system for studying Paneth cell secretion in real-time	884:948	Here, we report that microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen provides an ex vivo system for studying Paneth cell secretion in real-time.					
30804449	4	13	theme	lipopolysaccharide	826:843	arg1	microinjection					796:809	microinjection	796:809	microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen	796:873	Here, we report that microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen provides an ex vivo system for studying Paneth cell secretion in real-time.					
30804449	6	14	theme	Paneth	1135:1140	arg1	cells					1142:1146	Paneth cells	1135:1146	Paneth cells	1135:1146	However, Paneth cells did not respond to LPS delivered in culture media to enteroid exterior basolateral surface, although they responded to basolateral carbamyl choline.					
30804449	6	15	theme	basolateral	1219:1229	arg1	surface					1231:1237	enteroid exterior basolateral surface	1201:1237	enteroid exterior basolateral surface	1201:1237	However, Paneth cells did not respond to LPS delivered in culture media to enteroid exterior basolateral surface, although they responded to basolateral carbamyl choline.					
30804449	0	16	theme	cell	7:10	arg1	dynamics					20:27	Paneth cell granule dynamics	0:27	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids	0:84	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids.					
30804449	4	17	dep	ex	887:888	arg1	vivo					890:893	vivo	890:893	vivo	890:893	Here, we report that microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen provides an ex vivo system for studying Paneth cell secretion in real-time.					
30804449	3	18	theme	cell	604:607	arg1	transport					633:641	ion transport	629:641	ion transport	629:641	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	3	18	theme	cell	604:607	arg1	functions					609:617	intestinal epithelial cell functions	582:617	intestinal epithelial cell functions including ion transport	582:641	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	0	19	theme	Paneth	0:5	arg1	dynamics					20:27	Paneth cell granule dynamics	0:27	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids	0:84	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids.					
30804449	1	20	theme	Paneth	87:92	arg1	cells					94:98	Paneth cells	87:98	Paneth cells at the base of small intestinal crypts	87:137	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	1	21	from	base	107:110	arg1	cells					94:98	Paneth cells	87:98	Paneth cells at the base of small intestinal crypts	87:137	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	3	22	theme	intestinal	582:591	arg1	transport					633:641	ion transport	629:641	ion transport	629:641	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	3	22	theme	intestinal	582:591	arg1	functions					609:617	intestinal epithelial cell functions	582:617	intestinal epithelial cell functions including ion transport	582:641	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	8	23	theme	Paneth	1465:1470	arg1	responses					1487:1495	apically-induced Paneth cell secretory responses	1448:1495	apically-induced Paneth cell secretory responses in regulating the intestinal environment	1448:1536	These findings provide new insight for apically-induced Paneth cell secretory responses in regulating the intestinal environment.					
30804449	0	24	theme	granule	12:18	arg1	dynamics					20:27	Paneth cell granule dynamics	0:27	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids	0:84	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids.					
30804449	6	25	theme	exterior	1210:1217	arg1	surface					1231:1237	enteroid exterior basolateral surface	1201:1237	enteroid exterior basolateral surface	1201:1237	However, Paneth cells did not respond to LPS delivered in culture media to enteroid exterior basolateral surface, although they responded to basolateral carbamyl choline.					
30804449	8	26	theme	apically-induced	1448:1463	arg1	responses					1487:1495	apically-induced Paneth cell secretory responses	1448:1495	apically-induced Paneth cell secretory responses in regulating the intestinal environment	1448:1536	These findings provide new insight for apically-induced Paneth cell secretory responses in regulating the intestinal environment.					
30804449	3	27	theme	cells	542:546	arg1	enteroids					473:481	enteroids	473:481	enteroids	473:481	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	3	27	theme	cells	542:546	arg1	cultures					502:509	three-dimensional cultures	484:509	three-dimensional cultures of small intestinal epithelial cells	484:546	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	3	28	theme	three-dimensional	484:500	arg1	enteroids					473:481	enteroids	473:481	enteroids	473:481	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	3	28	theme	three-dimensional	484:500	arg1	cultures					502:509	three-dimensional cultures	484:509	three-dimensional cultures of small intestinal epithelial cells	484:546	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	0	29	theme	secretory	32:40	arg1	responses					42:50	secretory responses	32:50	secretory responses to bacterial stimuli in enteroids	32:84	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids.					
30804449	3	30	theme	intestinal	520:529	arg1	cells					542:546	small intestinal epithelial cells	514:546	small intestinal epithelial cells	514:546	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	8	31	theme	intestinal	1515:1524	arg1	environment					1526:1536	the intestinal environment	1511:1536	the intestinal environment	1511:1536	These findings provide new insight for apically-induced Paneth cell secretory responses in regulating the intestinal environment.					
30804449	5	32	theme	cells	1064:1068	arg1	surfaces					1032:1039	the apical surfaces	1021:1039	the apical surfaces of enteroid epithelial cells	1021:1068	The results show that Paneth cells released granules immediately when the apical surfaces of enteroid epithelial cells were exposed to LPS or live bacteria by microinjection.					
30804449	8	33	theme	cell	1472:1475	arg1	responses					1487:1495	apically-induced Paneth cell secretory responses	1448:1495	apically-induced Paneth cell secretory responses in regulating the intestinal environment	1448:1536	These findings provide new insight for apically-induced Paneth cell secretory responses in regulating the intestinal environment.					
30804449	0	34	from	stimuli	65:71	arg1	enteroids					76:84	enteroids	76:84	enteroids	76:84	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids.					
30804449	4	35	theme	enteroid	860:867	arg1	lumen					869:873	the enteroid lumen	856:873	the enteroid lumen	856:873	Here, we report that microinjection of bacteria or lipopolysaccharide (LPS) into the enteroid lumen provides an ex vivo system for studying Paneth cell secretion in real-time.					
30804449	1	36	theme	intestinal	311:320	arg1	microbiota					322:331	the intestinal microbiota	307:331	the intestinal microbiota	307:331	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	1	37	contain	containing	156:165	arg2	α-defensins					167:177	α-defensins	167:177	α-defensins	167:177	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	1	37	contain	containing	156:165	arg1	granules					147:154	granules	147:154	granules containing α-defensins	147:177	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	6	38	theme	basolateral	1267:1277	arg1	choline					1288:1294	basolateral carbamyl choline	1267:1294	basolateral carbamyl choline	1267:1294	However, Paneth cells did not respond to LPS delivered in culture media to enteroid exterior basolateral surface, although they responded to basolateral carbamyl choline.					
30804449	2	39	theme	cell	350:353	arg1	responses					365:373	Paneth cell secretory responses	343:373	Paneth cell secretory responses	343:373	However, Paneth cell secretory responses remain debatable and the mechanisms that regulate the secretion are not well understood.					
30804449	1	40	theme	microbiota	322:331	arg1	composition					292:302	the composition	288:302	the composition of the intestinal microbiota	288:331	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	3	41	theme	intestinal	752:761	arg1	microbiota					763:772	the intestinal microbiota	748:772	the intestinal microbiota	748:772	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	3	42	theme	Paneth	731:736	arg1	cells					738:742	Paneth cells	731:742	Paneth cells	731:742	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	3	43	theme	ion	629:631	arg1	transport					633:641	ion transport	629:641	ion transport	629:641	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	2	44	theme	Paneth	343:348	arg1	responses					365:373	Paneth cell secretory responses	343:373	Paneth cell secretory responses	343:373	However, Paneth cell secretory responses remain debatable and the mechanisms that regulate the secretion are not well understood.					
30804449	3	45	theme	closed	650:655	arg1	structures					657:666	their closed structures	644:666	their closed structures	644:666	Although enteroids, three-dimensional cultures of small intestinal epithelial cells, have proven useful for analyzing intestinal epithelial cell functions including ion transport, their closed structures have imposed limitations to investigating interactions between Paneth cells and the intestinal microbiota.					
30804449	5	46	theme	live	1093:1096	arg1	bacteria					1098:1105	live bacteria	1093:1105	live bacteria	1093:1105	The results show that Paneth cells released granules immediately when the apical surfaces of enteroid epithelial cells were exposed to LPS or live bacteria by microinjection.					
30804449	6	47	theme	carbamyl	1279:1286	arg1	choline					1288:1294	basolateral carbamyl choline	1267:1294	basolateral carbamyl choline	1267:1294	However, Paneth cells did not respond to LPS delivered in culture media to enteroid exterior basolateral surface, although they responded to basolateral carbamyl choline.					
30804449	7	48	theme	Paneth	1310:1315	arg1	cells					1317:1321	Paneth cells	1310:1321	Paneth cells	1310:1321	In addition, Paneth cells replenished their granules after secretion, enabling responses to second stimulation.					
30804449	6	49	theme	culture	1184:1190	arg1	media					1192:1196	culture media	1184:1196	culture media	1184:1196	However, Paneth cells did not respond to LPS delivered in culture media to enteroid exterior basolateral surface, although they responded to basolateral carbamyl choline.					
30804449	6	50	theme	enteroid	1201:1208	arg1	surface					1231:1237	enteroid exterior basolateral surface	1201:1237	enteroid exterior basolateral surface	1201:1237	However, Paneth cells did not respond to LPS delivered in culture media to enteroid exterior basolateral surface, although they responded to basolateral carbamyl choline.					
30804449	5	51	theme	enteroid	1044:1051	arg1	cells					1064:1068	enteroid epithelial cells	1044:1068	enteroid epithelial cells	1044:1068	The results show that Paneth cells released granules immediately when the apical surfaces of enteroid epithelial cells were exposed to LPS or live bacteria by microinjection.					
30804449	1	52	theme	small	115:119	arg1	crypts					132:137	small intestinal crypts	115:137	small intestinal crypts	115:137	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	1	53	theme	intestinal	220:229	arg1	environment					231:241	the intestinal environment	216:241	the intestinal environment	216:241	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	5	54	theme	epithelial	1053:1062	arg1	cells					1064:1068	enteroid epithelial cells	1044:1068	enteroid epithelial cells	1044:1068	The results show that Paneth cells released granules immediately when the apical surfaces of enteroid epithelial cells were exposed to LPS or live bacteria by microinjection.					
30804449	1	55	theme	intestinal	121:130	arg1	crypts					132:137	small intestinal crypts	115:137	small intestinal crypts	115:137	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	7	56	theme	second	1389:1394	arg1	stimulation					1396:1406	second stimulation	1389:1406	second stimulation	1389:1406	In addition, Paneth cells replenished their granules after secretion, enabling responses to second stimulation.					
30804449	0	57	theme	bacterial	55:63	arg1	stimuli					65:71	bacterial stimuli	55:71	bacterial stimuli in enteroids	55:84	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids.					
30804449	0	58	from	dynamics	20:27	arg1	responses					42:50	secretory responses	32:50	secretory responses to bacterial stimuli in enteroids	32:84	Paneth cell granule dynamics on secretory responses to bacterial stimuli in enteroids.					
30804449	1	59	dep	bacteria	194:201	arg1	response					182:189	response	182:189	response	182:189	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30804449	1	60	theme	crypts	132:137	arg1	base					107:110	the base	103:110	the base of small intestinal crypts	103:137	Paneth cells at the base of small intestinal crypts secrete granules containing α-defensins in response to bacteria and maintain the intestinal environment by clearing enteric pathogens and regulating the composition of the intestinal microbiota.					
30573732	0	0	theme	ischemia	102:109	arg1	therapy					111:117	ischemia therapy	102:117	ischemia therapy	102:117	High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.					
30573732	4	1	theme	hyaluronic	779:788	arg1	acid					790:793	a biodegradable catechol-conjugated hyaluronic acid	743:793	a biodegradable catechol-conjugated hyaluronic acid hydrogel	743:802	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	5	2	theme	therapeutic	931:941	arg1	effects					943:949	The therapeutic effects	927:949	The therapeutic effects of the fabricated vessel constructs	927:985	The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue.					
30573732	1	3	theme	clinical	175:182	arg1	treatment					213:221	disease treatment	205:221	disease treatment	205:221	The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery.					
30573732	1	3	theme	clinical	175:182	arg1	discovery					232:240	drug discovery	227:240	drug discovery	227:240	The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery.					
30573732	1	3	theme	clinical	175:182	arg1	applications					184:195	clinical applications	175:195	clinical applications such as disease treatment and drug discovery	175:240	The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery.					
30573732	4	4	theme	Co-aligned	643:652	arg1	cells					687:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	5	5	theme	ischemia	1028:1035	arg1	model					1043:1047	an ischemia mouse model	1025:1047	an ischemia mouse model	1025:1047	The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue.					
30573732	6	6	theme	artificial	1164:1173	arg1	tissues					1175:1181	artificial tissues	1164:1181	artificial tissues	1164:1181	Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.					
30573732	4	7	theme	catechol-conjugated	759:777	arg1	acid					790:793	a biodegradable catechol-conjugated hyaluronic acid	743:793	a biodegradable catechol-conjugated hyaluronic acid hydrogel	743:802	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	5	8	theme	tissue	1098:1103	arg1	recovery					1078:1085	the remarkable recovery	1063:1085	the remarkable recovery of damaged tissue	1063:1103	The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue.					
30573732	4	9	theme	umbilical	660:668	arg1	cells					687:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	4	10	theme	human	697:701	arg1	cells					716:720	human adipose stem cells	697:720	human adipose stem cells	697:720	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	3	11	theme	three-dimensional	589:605	arg1	distribution					618:629	a three-dimensional collateral distribution	587:629	a three-dimensional collateral distribution of vessels	587:640	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
30573732	2	12	theme	mechanical	381:390	arg1	properties					392:401	mechanical properties	381:401	mechanical properties	381:401	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	4	13	theme	human	654:658	arg1	cells					687:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	2	14	theme	tissue	443:448	arg1	function					450:457	proper tissue function	436:457	proper tissue function	436:457	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	2	15	theme	tissues	308:314	arg1	environments					292:303	the mechanical environments	277:303	the mechanical environments	277:303	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	2	16	theme	critical	409:416	arg1	roles					418:422	critical roles	409:422	critical roles	409:422	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	5	17	theme	fabricated	958:967	arg1	constructs					976:985	the fabricated vessel constructs	954:985	the fabricated vessel constructs	954:985	The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue.					
30573732	4	18	theme	vein	670:673	arg1	cells					687:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	6	19	theme	original	1198:1205	arg1	functions					1207:1215	the original functions	1194:1215	the original functions as well as structures	1194:1237	Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.					
30573732	5	20	theme	vessel	969:974	arg1	constructs					976:985	the fabricated vessel constructs	954:985	the fabricated vessel constructs	954:985	The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue.					
30573732	0	21	theme	acoustophoretic	16:30	arg1	patterning					40:49	High-resolution acoustophoretic 3D cell patterning	0:49	High-resolution acoustophoretic 3D cell patterning	0:49	High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.					
30573732	3	22	theme	vascular	560:567	arg1	tissue					569:574	therapeutic vascular tissue	548:574	therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels	548:640	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
30573732	4	23	theme	gene	841:844	arg1	expression					846:855	gene expression	841:855	gene expression	841:855	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	1	24	theme	disease	205:211	arg1	treatment					213:221	disease treatment	205:221	disease treatment	205:221	The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery.					
30573732	0	25	theme	High-resolution	0:14	arg1	patterning					40:49	High-resolution acoustophoretic 3D cell patterning	0:49	High-resolution acoustophoretic 3D cell patterning	0:49	High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.					
30573732	2	26	theme	cell	341:344	arg1	patterns					346:353	geometric cell patterns	331:353	geometric cell patterns	331:353	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	6	27	theme	tissues	1175:1181	arg1	types					1155:1159	various types	1147:1159	various types of artificial tissues that mimic the original functions as well as structures	1147:1237	Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.					
30573732	0	28	theme	cell	35:38	arg1	patterning					40:49	High-resolution acoustophoretic 3D cell patterning	0:49	High-resolution acoustophoretic 3D cell patterning	0:49	High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.					
30573732	4	29	theme	cell-cell	821:829	arg1	contacts					831:838	enhanced cell-cell contacts	812:838	enhanced cell-cell contacts	812:838	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	2	30	theme	geometric	331:339	arg1	patterns					346:353	geometric cell patterns	331:353	geometric cell patterns	331:353	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	3	31	theme	acoustophoretic	480:494	arg1	technique					496:504	an acoustophoretic technique	477:504	an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels	477:640	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
30573732	0	32	theme	3D	32:33	arg1	patterning					40:49	High-resolution acoustophoretic 3D cell patterning	0:49	High-resolution acoustophoretic 3D cell patterning	0:49	High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.					
30573732	5	33	theme	damaged	1090:1096	arg1	tissue					1098:1103	damaged tissue	1090:1103	damaged tissue	1090:1103	The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue.					
30573732	5	34	theme	remarkable	1067:1076	arg1	recovery					1078:1085	the remarkable recovery	1063:1085	the remarkable recovery of damaged tissue	1063:1103	The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue.					
30573732	1	35	theme	drug	227:230	arg1	discovery					232:240	drug discovery	227:240	drug discovery	227:240	The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery.					
30573732	4	36	theme	acid	790:793	arg1	hydrogel					795:802	a biodegradable catechol-conjugated hyaluronic acid hydrogel	743:802	a biodegradable catechol-conjugated hyaluronic acid hydrogel	743:802	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	4	37	theme	factors	918:924	arg1	contacts					831:838	enhanced cell-cell contacts	812:838	enhanced cell-cell contacts	812:838	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	4	37	theme	factors	918:924	arg1	expression					846:855	gene expression	841:855	gene expression	841:855	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	4	37	theme	factors	918:924	arg1	secretion					862:870	secretion	862:870	secretion of angiogenic and anti-inflammatory paracrine factors	862:924	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	2	38	theme	proper	436:441	arg1	function					450:457	proper tissue function	436:457	proper tissue function	436:457	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	4	39	theme	biodegradable	745:757	arg1	acid					790:793	a biodegradable catechol-conjugated hyaluronic acid	743:793	a biodegradable catechol-conjugated hyaluronic acid hydrogel	743:802	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	3	40	contain	containing	576:585	arg1	tissue					569:574	therapeutic vascular tissue	548:574	therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels	548:640	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
30573732	3	40	contain	containing	576:585	arg2	distribution					618:629	a three-dimensional collateral distribution	587:629	a three-dimensional collateral distribution of vessels	587:640	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
30573732	5	41	theme	constructs	976:985	arg1	effects					943:949	The therapeutic effects	927:949	The therapeutic effects of the fabricated vessel constructs	927:985	The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue.					
30573732	4	42	theme	paracrine	908:916	arg1	factors					918:924	angiogenic and anti-inflammatory paracrine factors	875:924	angiogenic and anti-inflammatory paracrine factors	875:924	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	4	43	theme	stem	711:714	arg1	cells					716:720	human adipose stem cells	697:720	human adipose stem cells	697:720	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	0	44	theme	functional	64:73	arg1	cylindroids					86:96	functional collateral cylindroids	64:96	functional collateral cylindroids for ischemia therapy	64:117	High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.					
30573732	3	45	theme	acoustic	520:527	arg1	waves					529:533	surface acoustic waves	512:533	surface acoustic waves	512:533	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
30573732	2	46	theme	Recent	243:248	arg1	studies					250:256	Recent studies	243:256	Recent studies	243:256	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	1	47	theme	functional	139:148	arg1	tissues					150:156	functional tissues	139:156	functional tissues	139:156	The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery.					
30573732	4	48	theme	enhanced	812:819	arg1	contacts					831:838	enhanced cell-cell contacts	812:838	enhanced cell-cell contacts	812:838	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	4	49	theme	angiogenic	875:884	arg1	factors					918:924	angiogenic and anti-inflammatory paracrine factors	875:924	angiogenic and anti-inflammatory paracrine factors	875:924	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	1	50	theme	tissues	150:156	arg1	essential					161:169	essential	161:169	essential	161:169	The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery.					
30573732	1	50	theme	tissues	150:156	arg1	fabrication					124:134	The fabrication	120:134	The fabrication of functional tissues	120:156	The fabrication of functional tissues is essential for clinical applications such as disease treatment and drug discovery.					
30573732	3	51	theme	collateral	607:616	arg1	distribution					618:629	a three-dimensional collateral distribution	587:629	a three-dimensional collateral distribution of vessels	587:640	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
30573732	4	52	theme	endothelial	675:685	arg1	cells					687:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells	643:691	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	5	53	theme	mouse	1037:1041	arg1	model					1043:1047	an ischemia mouse model	1025:1047	an ischemia mouse model	1025:1047	The therapeutic effects of the fabricated vessel constructs are demonstrated in experiments using an ischemia mouse model by exhibiting the remarkable recovery of damaged tissue.					
30573732	2	54	theme	mechanical	281:290	arg1	environments					292:303	the mechanical environments	277:303	the mechanical environments	277:303	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	3	55	theme	surface	512:518	arg1	waves					529:533	surface acoustic waves	512:533	surface acoustic waves	512:533	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
30573732	2	56	theme	material	356:363	arg1	composition					365:375	material composition	356:375	material composition	356:375	Recent studies have revealed that the mechanical environments of tissues, determined by geometric cell patterns, material composition, or mechanical properties, play critical roles in ensuring proper tissue function.					
30573732	6	57	theme	various	1147:1153	arg1	types					1155:1159	various types	1147:1159	various types of artificial tissues that mimic the original functions as well as structures	1147:1237	Our study can be referenced to fabricate various types of artificial tissues that mimic the original functions as well as structures.					
30573732	4	58	theme	anti-inflammatory	890:906	arg1	factors					918:924	angiogenic and anti-inflammatory paracrine factors	875:924	angiogenic and anti-inflammatory paracrine factors	875:924	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	4	59	theme	adipose	703:709	arg1	cells					716:720	human adipose stem cells	697:720	human adipose stem cells	697:720	Co-aligned human umbilical vein endothelial cells and human adipose stem cells that are arranged in a biodegradable catechol-conjugated hyaluronic acid hydrogel exhibit enhanced cell-cell contacts, gene expression, and secretion of angiogenic and anti-inflammatory paracrine factors.					
30573732	3	60	theme	therapeutic	548:558	arg1	tissue					569:574	therapeutic vascular tissue	548:574	therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels	548:640	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
30573732	0	61	theme	collateral	75:84	arg1	cylindroids					86:96	functional collateral cylindroids	64:96	functional collateral cylindroids for ischemia therapy	64:117	High-resolution acoustophoretic 3D cell patterning to construct functional collateral cylindroids for ischemia therapy.					
30573732	3	62	theme	vessels	634:640	arg1	distribution					618:629	a three-dimensional collateral distribution	587:629	a three-dimensional collateral distribution of vessels	587:640	Here, we propose an acoustophoretic technique using surface acoustic waves to fabricate therapeutic vascular tissue containing a three-dimensional collateral distribution of vessels.					
31780730	5	0	from	speckles	1028:1035	arg1	tobacco					1040:1046	tobacco leaves	1040:1053	tobacco leaves	1040:1053	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	8	1	theme	flue-cured	1537:1546	arg1	tobacco					1548:1554	flue-cured tobacco	1537:1554	flue-cured tobacco	1537:1554	In addition, other indices show different trends for different cultivars of flue-cured tobacco.					
31780730	2	2	dep	K326	505:508	arg1	Hongda					543:548	Hongda	543:548	Hongda	543:548	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	2	dep	K326	505:508	arg1	Yunyan87					511:518	Yunyan87	511:518	Yunyan87	511:518	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	4	3	theme	matter	925:930	arg1	structure					903:911	the structure	899:911	the structure of the grey matter in terms of chemical composition	899:963	Moreover, by utilising thin-layer chromatography and high-performance liquid chromatography, we analysed and identified the structure of the grey matter in terms of chemical composition.					
31780730	10	4	theme	mechanisms	1775:1784	arg1	study					1757:1761	the study	1753:1761	the study of browning mechanisms in tobacco leaves	1753:1802	The research is important to the study of browning mechanisms in tobacco leaves and provides corresponding targets for strategies to reduce browning thereof.					
31780730	1	5	theme	tobacco	254:260	arg1	value					220:224	value	220:224	value	220:224	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	1	5	theme	tobacco	254:260	arg1	usability					241:249	industrial usability	230:249	industrial usability	230:249	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	9	6	from	substance	1658:1666	arg1	tobacco					1698:1704	tobacco leaves	1698:1711	tobacco leaves	1698:1711	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	1	7	theme	grey	169:172	arg1	speckles					174:181	grey speckles	169:181	grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves	169:267	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	5	8	from	tobacco	1040:1046	arg1	component					1010:1018	the main component	1001:1018	the main component of grey speckles on tobacco leaves	1001:1053	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	5	8	from	tobacco	1040:1046	arg1	3-acetyl-6,7-dimethoxycoumarin					1058:1087	3-acetyl-6,7-dimethoxycoumarin	1058:1087	3-acetyl-6,7-dimethoxycoumarin (YC-ZJF)	1058:1096	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	2	9	dep	%	607:607	arg1	to					602:603	to	602:603	to	602:603	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	6	10	theme	total	1138:1142	arg1	value					1177:1181	output value	1170:1181	output value	1170:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	10	theme	total	1138:1142	arg1	sugar					1144:1148	total sugar	1138:1148	total sugar	1138:1148	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	2	11	dep	BDs	575:577	arg1	%					583:583	<25%	580:583	<25%	580:583	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	11	dep	BDs	575:577	arg1	%					617:617	>75%	614:617	>75%	614:617	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	11	dep	BDs	575:577	arg1	%					595:595	25% to 50%	586:595	25% to 50%	586:595	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	11	dep	BDs	575:577	arg1	%					607:607	50% to 75%	598:607	50% to 75%	598:607	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	0	12	theme	reaction	110:117	arg1	products					119:126	reaction products	110:126	reaction products	110:126	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	3	13	theme	related	629:635	arg1	Indices					621:627	Indices	621:627	Indices related to	621:638	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	6	14	theme	output	1170:1175	arg1	value					1177:1181	output value	1170:1181	output value	1170:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	14	theme	output	1170:1175	arg1	sugar					1144:1148	total sugar	1138:1148	total sugar	1138:1148	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	10	15	from	mechanisms	1775:1784	arg1	tobacco					1789:1795	tobacco leaves	1789:1802	tobacco leaves	1789:1802	The research is important to the study of browning mechanisms in tobacco leaves and provides corresponding targets for strategies to reduce browning thereof.					
31780730	7	16	from	changes	1376:1382	arg1	protein					1387:1393	protein	1387:1393	protein	1387:1393	The changes in protein, total nitrogen, and nicotine are insignificant with changing BD.					
31780730	7	16	from	changes	1376:1382	arg1	nitrogen					1402:1409	total nitrogen	1396:1409	total nitrogen	1396:1409	The changes in protein, total nitrogen, and nicotine are insignificant with changing BD.					
31780730	7	16	from	changes	1376:1382	arg1	nicotine					1416:1423	nicotine	1416:1423	nicotine	1416:1423	The changes in protein, total nitrogen, and nicotine are insignificant with changing BD.					
31780730	10	17	theme	corresponding	1817:1829	arg1	targets					1831:1837	corresponding targets	1817:1837	corresponding targets for strategies	1817:1852	The research is important to the study of browning mechanisms in tobacco leaves and provides corresponding targets for strategies to reduce browning thereof.					
31780730	6	18	theme	sugar	1163:1167	arg1	value					1177:1181	output value	1170:1181	output value	1170:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	18	theme	sugar	1163:1167	arg1	cultivars					1288:1296	the three cultivars	1278:1296	the three cultivars	1278:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	18	theme	sugar	1163:1167	arg1	score					1269:1273	sensory evaluation score	1250:1273	sensory evaluation score of the three cultivars	1250:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	18	theme	sugar	1163:1167	arg1	index					1239:1243	shatter resistance index	1220:1243	shatter resistance index	1220:1243	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	18	theme	sugar	1163:1167	arg1	sugar					1163:1167	reducing sugar	1154:1167	reducing sugar	1154:1167	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	18	theme	sugar	1163:1167	arg1	proportion					1188:1197	the proportion	1184:1197	the proportion of superior tobacco	1184:1217	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	18	theme	sugar	1163:1167	arg1	amount					1128:1133	the amount	1124:1133	the amount of total sugar and reducing sugar, output value	1124:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	18	theme	sugar	1163:1167	arg1	tobacco					1211:1217	superior tobacco	1202:1217	superior tobacco	1202:1217	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	18	theme	sugar	1163:1167	arg1	sugar					1144:1148	total sugar	1138:1148	total sugar	1138:1148	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	1	19	dep	tobacco	254:260	arg1	leaves					262:267	leaves	262:267	leaves	262:267	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	9	20	attach	derived	1668:1674	arg2	substance					1658:1666	the substance	1654:1666	the substance derived from grey speckles on tobacco leaves	1654:1711	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	9	20	attach	derived	1668:1674	arg2	YC-ZJF					1716:1721	YC-ZJF	1716:1721	YC-ZJF	1716:1721	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	9	20	attach	derived	1668:1674	arg1	speckles					1686:1693	grey speckles	1681:1693	grey speckles	1681:1693	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	1	21	from	speckles	174:181	arg1	tobacco					186:192	tobacco leaves	186:199	tobacco leaves	186:199	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	4	22	theme	high-performance	832:847	arg1	chromatography					856:869	high-performance liquid chromatography	832:869	high-performance liquid chromatography	832:869	Moreover, by utilising thin-layer chromatography and high-performance liquid chromatography, we analysed and identified the structure of the grey matter in terms of chemical composition.					
31780730	6	23	theme	resistance	1228:1237	arg1	index					1239:1243	shatter resistance index	1220:1243	shatter resistance index	1220:1243	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	1	24	theme	browning	140:147	arg1	reaction					149:156	The enzyme browning reaction	129:156	The enzyme browning reaction	129:156	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	3	25	theme	tobacco	723:729	arg1	traits					650:655	economic traits	641:655	economic traits	641:655	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	3	25	theme	tobacco	723:729	arg1	Indices					621:627	Indices	621:627	Indices related to	621:638	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	3	25	theme	tobacco	723:729	arg1	properties					688:697	physical properties	679:697	physical properties	679:697	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	3	25	theme	tobacco	723:729	arg1	quality					712:718	sensory quality	704:718	sensory quality of tobacco leaves with different cultivars	704:761	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	3	25	theme	tobacco	723:729	arg1	components					667:676	chemical components	658:676	chemical components	658:676	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	9	26	theme	speckled	1619:1626	arg1	components					1600:1609	the components	1596:1609	the components of grey speckled leaves	1596:1633	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	3	27	with	tobacco	723:729	arg1	cultivars					753:761	different cultivars	743:761	different cultivars	743:761	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	5	28	dep	tobacco	1040:1046	arg1	leaves					1048:1053	leaves	1048:1053	leaves	1048:1053	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	3	29	theme	chemical	658:665	arg1	components					667:676	chemical components	658:676	chemical components	658:676	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	6	30	theme	superior	1202:1209	arg1	tobacco					1211:1217	superior tobacco	1202:1217	superior tobacco	1202:1217	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	2	31	theme	flue-cured	475:484	arg1	cultivars					494:502	three flue-cured tobacco cultivars	469:502	three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda))	469:550	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	0	32	theme	components	96:105	arg1	identification					78:91	identification	78:91	identification of components of reaction products	78:126	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	0	32	theme	components	96:105	arg1	usability					46:54	the usability	42:54	the usability of tobacco leaves	42:72	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	5	33	theme	experimental	970:981	arg1	results					983:989	The experimental results	966:989	The experimental results	966:989	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	9	34	theme	components	1600:1609	arg1	identification					1578:1591	identification	1578:1591	identification	1578:1591	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	9	34	theme	components	1600:1609	arg1	separation					1563:1572	separation	1563:1572	separation	1563:1572	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	2	35	dep	degrees	322:328	arg1	leaves					347:352	leaves	347:352	leaves on the usability of different cultivars and positions	347:406	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	3	36	dep	tobacco	723:729	arg1	leaves					731:736	leaves	731:736	leaves	731:736	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	2	37	theme	positions	398:406	arg1	usability					361:369	the usability	357:369	the usability of different cultivars and positions	357:406	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	0	38	theme	enzymatic	11:19	arg1	reaction					30:37	enzymatic browning reaction	11:37	enzymatic browning reaction	11:37	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	2	39	theme	cultivars	384:392	arg1	usability					361:369	the usability	357:369	the usability of different cultivars and positions	357:406	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	6	40	theme	sensory	1250:1256	arg1	score					1269:1273	sensory evaluation score	1250:1273	sensory evaluation score of the three cultivars	1250:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	0	41	theme	reaction	30:37	arg1	Effects					0:6	Effects	0:6	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.	0:127	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	9	42	theme	grey	1681:1684	arg1	speckles					1686:1693	grey speckles	1681:1693	grey speckles	1681:1693	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	5	43	theme	grey	1023:1026	arg1	speckles					1028:1035	grey speckles	1023:1035	grey speckles on tobacco leaves	1023:1053	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	2	44	theme	degrees	322:328	arg1	influences					289:298	the influences	285:298	the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions	285:406	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	6	45	theme	starch	1332:1337	arg1	content					1339:1345	the starch content	1328:1345	the starch content	1328:1345	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	4	46	theme	composition	953:963	arg1	terms					935:939	terms	935:939	terms of chemical composition	935:963	Moreover, by utilising thin-layer chromatography and high-performance liquid chromatography, we analysed and identified the structure of the grey matter in terms of chemical composition.					
31780730	1	47	dep	tobacco	186:192	arg1	leaves					194:199	leaves	194:199	leaves	194:199	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	4	48	theme	grey	920:923	arg1	matter					925:930	the grey matter	916:930	the grey matter	916:930	Moreover, by utilising thin-layer chromatography and high-performance liquid chromatography, we analysed and identified the structure of the grey matter in terms of chemical composition.					
31780730	6	49	theme	sugar	1144:1148	arg1	value					1177:1181	output value	1170:1181	output value	1170:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	49	theme	sugar	1144:1148	arg1	cultivars					1288:1296	the three cultivars	1278:1296	the three cultivars	1278:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	49	theme	sugar	1144:1148	arg1	score					1269:1273	sensory evaluation score	1250:1273	sensory evaluation score of the three cultivars	1250:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	49	theme	sugar	1144:1148	arg1	index					1239:1243	shatter resistance index	1220:1243	shatter resistance index	1220:1243	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	49	theme	sugar	1144:1148	arg1	sugar					1163:1167	reducing sugar	1154:1167	reducing sugar	1154:1167	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	49	theme	sugar	1144:1148	arg1	proportion					1188:1197	the proportion	1184:1197	the proportion of superior tobacco	1184:1217	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	49	theme	sugar	1144:1148	arg1	amount					1128:1133	the amount	1124:1133	the amount of total sugar and reducing sugar, output value	1124:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	49	theme	sugar	1144:1148	arg1	tobacco					1211:1217	superior tobacco	1202:1217	superior tobacco	1202:1217	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	49	theme	sugar	1144:1148	arg1	sugar					1144:1148	total sugar	1138:1148	total sugar	1138:1148	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	8	50	theme	different	1514:1522	arg1	cultivars					1524:1532	different cultivars	1514:1532	different cultivars of flue-cured tobacco	1514:1554	In addition, other indices show different trends for different cultivars of flue-cured tobacco.					
31780730	1	51	theme	industrial	230:239	arg1	usability					241:249	industrial usability	230:249	industrial usability	230:249	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	5	52	from	component	1010:1018	arg1	tobacco					1040:1046	tobacco leaves	1040:1053	tobacco leaves	1040:1053	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	6	53	theme	cultivars	1288:1296	arg1	value					1177:1181	output value	1170:1181	output value	1170:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	53	theme	cultivars	1288:1296	arg1	cultivars					1288:1296	the three cultivars	1278:1296	the three cultivars	1278:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	53	theme	cultivars	1288:1296	arg1	score					1269:1273	sensory evaluation score	1250:1273	sensory evaluation score of the three cultivars	1250:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	53	theme	cultivars	1288:1296	arg1	index					1239:1243	shatter resistance index	1220:1243	shatter resistance index	1220:1243	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	53	theme	cultivars	1288:1296	arg1	sugar					1163:1167	reducing sugar	1154:1167	reducing sugar	1154:1167	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	53	theme	cultivars	1288:1296	arg1	proportion					1188:1197	the proportion	1184:1197	the proportion of superior tobacco	1184:1217	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	53	theme	cultivars	1288:1296	arg1	amount					1128:1133	the amount	1124:1133	the amount of total sugar and reducing sugar, output value	1124:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	53	theme	cultivars	1288:1296	arg1	tobacco					1211:1217	superior tobacco	1202:1217	superior tobacco	1202:1217	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	53	theme	cultivars	1288:1296	arg1	sugar					1144:1148	total sugar	1138:1148	total sugar	1138:1148	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	8	54	theme	other	1474:1478	arg1	indices					1480:1486	other indices	1474:1486	other indices	1474:1486	In addition, other indices show different trends for different cultivars of flue-cured tobacco.					
31780730	10	55	theme	browning	1766:1773	arg1	mechanisms					1775:1784	browning mechanisms	1766:1784	browning mechanisms in tobacco leaves	1766:1802	The research is important to the study of browning mechanisms in tobacco leaves and provides corresponding targets for strategies to reduce browning thereof.					
31780730	8	56	theme	tobacco	1548:1554	arg1	cultivars					1524:1532	different cultivars	1514:1532	different cultivars of flue-cured tobacco	1514:1554	In addition, other indices show different trends for different cultivars of flue-cured tobacco.					
31780730	2	57	theme	different	565:573	arg1	BDs					575:577	set four different BDs	556:577	set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%)	556:618	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	4	58	theme	liquid	849:854	arg1	chromatography					856:869	high-performance liquid chromatography	832:869	high-performance liquid chromatography	832:869	Moreover, by utilising thin-layer chromatography and high-performance liquid chromatography, we analysed and identified the structure of the grey matter in terms of chemical composition.					
31780730	6	59	theme	BD	1120:1121	arg1	increase					1108:1115	the increase	1104:1115	the increase of BD	1104:1121	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	2	60	theme	different	303:311	arg1	BDs					331:333	BDs	331:333	BDs	331:333	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	60	theme	different	303:311	arg1	degrees					322:328	different browning degrees	303:328	different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions	303:406	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	61	theme	set	556:558	arg1	BDs					575:577	set four different BDs	556:577	set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%)	556:618	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	0	62	theme	products	119:126	arg1	components					96:105	components	96:105	components of reaction products	96:126	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	1	63	dep	value	220:224	arg1	the					216:218	the	216:218	the	216:218	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	10	64	from	study	1757:1761	arg1	tobacco					1789:1795	tobacco leaves	1789:1802	tobacco leaves	1789:1802	The research is important to the study of browning mechanisms in tobacco leaves and provides corresponding targets for strategies to reduce browning thereof.					
31780730	2	65	theme	%	600:600	arg1	%					607:607	50% to 75%	598:607	50% to 75%	598:607	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	9	66	dep	speckled	1619:1626	arg1	leaves					1628:1633	leaves	1628:1633	leaves	1628:1633	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	0	67	from	Effects	0:6	arg1	identification					78:91	identification	78:91	identification of components of reaction products	78:126	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	0	67	from	Effects	0:6	arg1	usability					46:54	the usability	42:54	the usability of tobacco leaves	42:72	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	6	68	theme	reducing	1154:1161	arg1	sugar					1163:1167	reducing sugar	1154:1167	reducing sugar	1154:1167	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	3	69	theme	sensory	704:710	arg1	quality					712:718	sensory quality	704:718	sensory quality of tobacco leaves with different cultivars	704:761	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	0	70	theme	tobacco	59:65	arg1	identification					78:91	identification	78:91	identification of components of reaction products	78:126	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	0	70	theme	tobacco	59:65	arg1	usability					46:54	the usability	42:54	the usability of tobacco leaves	42:72	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	1	71	theme	enzyme	133:138	arg1	reaction					149:156	The enzyme browning reaction	129:156	The enzyme browning reaction	129:156	The enzyme browning reaction results in grey speckles on tobacco leaves, which impairs the value and industrial usability of tobacco leaves.					
31780730	3	72	theme	economic	641:648	arg1	traits					650:655	economic traits	641:655	economic traits	641:655	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	6	73	theme	shatter	1220:1226	arg1	index					1239:1243	shatter resistance index	1220:1243	shatter resistance index	1220:1243	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	10	74	from	tobacco	1789:1795	arg1	study					1757:1761	the study	1753:1761	the study of browning mechanisms in tobacco leaves	1753:1802	The research is important to the study of browning mechanisms in tobacco leaves and provides corresponding targets for strategies to reduce browning thereof.					
31780730	2	75	theme	matter	449:454	arg1	structure					423:431	structure	423:431	structure of brown (grey) matter	423:454	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	3	76	theme	physical	679:686	arg1	properties					688:697	physical properties	679:697	physical properties	679:697	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	6	77	theme	tobacco	1211:1217	arg1	value					1177:1181	output value	1170:1181	output value	1170:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	77	theme	tobacco	1211:1217	arg1	cultivars					1288:1296	the three cultivars	1278:1296	the three cultivars	1278:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	77	theme	tobacco	1211:1217	arg1	score					1269:1273	sensory evaluation score	1250:1273	sensory evaluation score of the three cultivars	1250:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	77	theme	tobacco	1211:1217	arg1	index					1239:1243	shatter resistance index	1220:1243	shatter resistance index	1220:1243	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	77	theme	tobacco	1211:1217	arg1	sugar					1163:1167	reducing sugar	1154:1167	reducing sugar	1154:1167	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	77	theme	tobacco	1211:1217	arg1	proportion					1188:1197	the proportion	1184:1197	the proportion of superior tobacco	1184:1217	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	77	theme	tobacco	1211:1217	arg1	amount					1128:1133	the amount	1124:1133	the amount of total sugar and reducing sugar, output value	1124:1181	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	77	theme	tobacco	1211:1217	arg1	tobacco					1211:1217	superior tobacco	1202:1217	superior tobacco	1202:1217	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	6	77	theme	tobacco	1211:1217	arg1	sugar					1144:1148	total sugar	1138:1148	total sugar	1138:1148	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	5	78	theme	main	1005:1008	arg1	component					1010:1018	the main component	1001:1018	the main component of grey speckles on tobacco leaves	1001:1053	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	5	78	theme	main	1005:1008	arg1	3-acetyl-6,7-dimethoxycoumarin					1058:1087	3-acetyl-6,7-dimethoxycoumarin	1058:1087	3-acetyl-6,7-dimethoxycoumarin (YC-ZJF)	1058:1096	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	7	79	theme	total	1396:1400	arg1	nitrogen					1402:1409	total nitrogen	1396:1409	total nitrogen	1396:1409	The changes in protein, total nitrogen, and nicotine are insignificant with changing BD.					
31780730	2	80	theme	tobacco	486:492	arg1	cultivars					494:502	three flue-cured tobacco cultivars	469:502	three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda))	469:550	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	10	81	dep	tobacco	1789:1795	arg1	leaves					1797:1802	leaves	1797:1802	leaves	1797:1802	The research is important to the study of browning mechanisms in tobacco leaves and provides corresponding targets for strategies to reduce browning thereof.					
31780730	9	82	dep	tobacco	1698:1704	arg1	leaves					1706:1711	leaves	1706:1711	leaves	1706:1711	After separation and identification of the components of grey speckled leaves, it is proved that the substance derived from grey speckles on tobacco leaves is YC-ZJF.					
31780730	4	83	theme	thin-layer	802:811	arg1	chromatography					813:826	thin-layer chromatography	802:826	thin-layer chromatography	802:826	Moreover, by utilising thin-layer chromatography and high-performance liquid chromatography, we analysed and identified the structure of the grey matter in terms of chemical composition.					
31780730	3	84	theme	different	743:751	arg1	cultivars					753:761	different cultivars	743:761	different cultivars	743:761	Indices related to: economic traits, chemical components, physical properties, and sensory quality of tobacco leaves with different cultivars were evaluated.					
31780730	2	85	theme	%	588:588	arg1	%					595:595	25% to 50%	586:595	25% to 50%	586:595	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	0	86	theme	browning	21:28	arg1	reaction					30:37	enzymatic browning reaction	11:37	enzymatic browning reaction	11:37	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	4	87	from	structure	903:911	arg1	terms					935:939	terms	935:939	terms of chemical composition	935:963	Moreover, by utilising thin-layer chromatography and high-performance liquid chromatography, we analysed and identified the structure of the grey matter in terms of chemical composition.					
31780730	0	88	dep	tobacco	59:65	arg1	leaves					67:72	leaves	67:72	leaves	67:72	Effects of enzymatic browning reaction on the usability of tobacco leaves and identification of components of reaction products.					
31780730	2	89	dep	%	595:595	arg1	to					590:591	to	590:591	to	590:591	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	6	90	theme	evaluation	1258:1267	arg1	score					1269:1273	sensory evaluation score	1250:1273	sensory evaluation score of the three cultivars	1250:1296	With the increase of BD, the amount of total sugar and reducing sugar, output value, the proportion of superior tobacco, shatter resistance index, and sensory evaluation score of the three cultivars significantly decrease, while the starch content increases significantly.					
31780730	2	91	dep	cultivars	494:502	arg1	K326					505:508	K326	505:508	K326	505:508	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	92	theme	grey	443:446	arg1	matter					449:454	brown (grey) matter	436:454	brown (grey) matter	436:454	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	93	theme	different	374:382	arg1	cultivars					384:392	different cultivars	374:392	different cultivars	374:392	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	94	theme	brown	436:440	arg1	matter					449:454	brown (grey) matter	436:454	brown (grey) matter	436:454	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	8	95	theme	different	1493:1501	arg1	trends					1503:1508	different trends	1493:1508	different trends for different cultivars of flue-cured tobacco	1493:1554	In addition, other indices show different trends for different cultivars of flue-cured tobacco.					
31780730	5	96	theme	speckles	1028:1035	arg1	component					1010:1018	the main component	1001:1018	the main component of grey speckles on tobacco leaves	1001:1053	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	5	96	theme	speckles	1028:1035	arg1	3-acetyl-6,7-dimethoxycoumarin					1058:1087	3-acetyl-6,7-dimethoxycoumarin	1058:1087	3-acetyl-6,7-dimethoxycoumarin (YC-ZJF)	1058:1096	The experimental results show that the main component of grey speckles on tobacco leaves is 3-acetyl-6,7-dimethoxycoumarin (YC-ZJF).					
31780730	2	97	theme	browning	313:320	arg1	BDs					331:333	BDs	331:333	BDs	331:333	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	97	theme	browning	313:320	arg1	degrees					322:328	different browning degrees	303:328	different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions	303:406	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	4	98	theme	chemical	944:951	arg1	composition					953:963	chemical composition	944:963	chemical composition	944:963	Moreover, by utilising thin-layer chromatography and high-performance liquid chromatography, we analysed and identified the structure of the grey matter in terms of chemical composition.					
31780730	2	99	theme	tobacco	339:345	arg1	BDs					331:333	BDs	331:333	BDs	331:333	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
31780730	2	99	theme	tobacco	339:345	arg1	degrees					322:328	different browning degrees	303:328	different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions	303:406	To demonstrate the influences of different browning degrees (BDs) of tobacco leaves on the usability of different cultivars and positions and identified structure of brown (grey) matter, we selected three flue-cured tobacco cultivars (K326, Yunyan87, and Honghuadajinyuan (Hongda)) and set four different BDs (<25%, 25% to 50%, 50% to 75%, and >75%).					
30389525	5	0	theme	wall	774:777	arg1	species					754:760	reactive oxygen species	738:760	reactive oxygen species (ROS)	738:766	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	0	theme	wall	774:777	arg1	reinforcement					779:791	cell wall reinforcement	769:791	cell wall reinforcement by lignin deposition	769:812	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	6	1	theme	yield	935:939	arg1	enhancement					941:951	57.8% yield enhancement	929:951	57.8% yield enhancement	929:951	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	6	2	theme	NPs	974:976	arg1	application					978:988	SA-CS NPs application	968:988	SA-CS NPs application	968:988	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	6	3	theme	rot	906:908	arg1	disease					917:923	post-flowering stalk rot (PFSR) disease	885:923	post-flowering stalk rot (PFSR) disease	885:923	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	6	4	from	field	861:865	arg1	evident					957:963	evident	957:963	evident	957:963	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	2	5	theme	chemical	334:341	arg1	composition					343:353	chemical composition	334:353	chemical composition	334:353	SA-CS NPs were characterised for colloidal size distribution, functional group, surface chemistry, chemical composition, crystal structure and morphology.					
30389525	6	6	theme	SA-CS	968:972	arg1	application					978:988	SA-CS NPs application	968:988	SA-CS NPs application	968:988	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	6	7	theme	stalk	900:904	arg1	disease					917:923	post-flowering stalk rot (PFSR) disease	885:923	post-flowering stalk rot (PFSR) disease	885:923	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	2	8	theme	size	278:281	arg1	distribution					283:294	colloidal size distribution	268:294	colloidal size distribution	268:294	SA-CS NPs were characterised for colloidal size distribution, functional group, surface chemistry, chemical composition, crystal structure and morphology.					
30389525	4	9	theme	physiological-biochemical	591:615	arg1	responses					617:625	significant physiological-biochemical responses	579:625	significant physiological-biochemical responses	579:625	Findings unveil that SA-CS NPs expressed significant physiological-biochemical responses in vitro and in vivo.					
30389525	6	10	theme	post-flowering	885:898	arg1	disease					917:923	post-flowering stalk rot (PFSR) disease	885:923	post-flowering stalk rot (PFSR) disease	885:923	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	2	11	theme	colloidal	268:276	arg1	distribution					283:294	colloidal size distribution	268:294	colloidal size distribution	268:294	SA-CS NPs were characterised for colloidal size distribution, functional group, surface chemistry, chemical composition, crystal structure and morphology.					
30389525	4	12	theme	significant	579:589	arg1	responses					617:625	significant physiological-biochemical responses	579:625	significant physiological-biochemical responses	579:625	Findings unveil that SA-CS NPs expressed significant physiological-biochemical responses in vitro and in vivo.					
30389525	3	13	theme	SA-CS	474:478	arg1	NPs					480:482	SA-CS NPs	474:482	SA-CS NPs	474:482	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	7	14	theme	NPs	1073:1075	arg1	biostimulant					1082:1093	a biostimulant	1080:1093	a biostimulant for plant disease control and higher yield	1080:1136	The obtained results claim commercial potential of SA-CS NPs as a biostimulant for plant disease control and higher yield.					
30389525	7	14	theme	NPs	1073:1075	arg1	potential					1054:1062	commercial potential	1043:1062	commercial potential of SA-CS NPs	1043:1075	The obtained results claim commercial potential of SA-CS NPs as a biostimulant for plant disease control and higher yield.					
30389525	3	15	theme	NPs	432:434	arg1	synthesis					436:444	SA-CS NPs synthesis	426:444	SA-CS NPs synthesis	426:444	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	7	16	theme	disease	1105:1111	arg1	control					1113:1119	plant disease control	1099:1119	plant disease control	1099:1119	The obtained results claim commercial potential of SA-CS NPs as a biostimulant for plant disease control and higher yield.					
30389525	6	17	from	evident	957:963	arg1	field					861:865	field	861:865	field	861:865	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	6	17	from	evident	957:963	arg1	application					978:988	SA-CS NPs application	968:988	SA-CS NPs application	968:988	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	6	18	from	application	978:988	arg1	evident					957:963	evident	957:963	evident	957:963	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	3	19	theme	promoting	516:524	arg1	activities					526:535	antifungal and seedling growth promoting activities	485:535	antifungal and seedling growth promoting activities	485:535	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	19	theme	promoting	516:524	arg1	method					416:421	a method	414:421	a method of SA-CS NPs synthesis	414:444	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	20	theme	synthesis	436:444	arg1	activities					526:535	antifungal and seedling growth promoting activities	485:535	antifungal and seedling growth promoting activities	485:535	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	20	theme	synthesis	436:444	arg1	profile					455:461	release profile	447:461	release profile of SA from SA-CS NPs	447:482	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	20	theme	synthesis	436:444	arg1	method					416:421	a method	414:421	a method of SA-CS NPs synthesis	414:444	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	0	21	theme	acid	10:13	arg1	nanoparticle					39:50	Salicylic acid functionalized chitosan nanoparticle	0:50	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.	0:89	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.					
30389525	6	22	theme	%	872:872	arg1	control					874:880	59.4% control	868:880	59.4% control of post-flowering stalk rot (PFSR) disease	868:923	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	7	23	theme	obtained	1020:1027	arg1	results					1029:1035	The obtained results	1016:1035	The obtained results	1016:1035	The obtained results claim commercial potential of SA-CS NPs as a biostimulant for plant disease control and higher yield.					
30389525	0	24	theme	Salicylic	0:8	arg1	nanoparticle					39:50	Salicylic acid functionalized chitosan nanoparticle	0:50	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.	0:89	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.					
30389525	7	25	theme	higher	1125:1130	arg1	yield					1132:1136	higher yield	1125:1136	higher yield	1125:1136	The obtained results claim commercial potential of SA-CS NPs as a biostimulant for plant disease control and higher yield.					
30389525	7	26	theme	SA-CS	1067:1071	arg1	NPs					1073:1075	SA-CS NPs	1067:1075	SA-CS NPs	1067:1075	The obtained results claim commercial potential of SA-CS NPs as a biostimulant for plant disease control and higher yield.					
30389525	2	27	theme	functional	297:306	arg1	group					308:312	functional group	297:312	functional group	297:312	SA-CS NPs were characterised for colloidal size distribution, functional group, surface chemistry, chemical composition, crystal structure and morphology.					
30389525	3	28	theme	SA-CS	426:430	arg1	synthesis					436:444	SA-CS NPs synthesis	426:444	SA-CS NPs synthesis	426:444	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	0	29	theme	chitosan	30:37	arg1	nanoparticle					39:50	Salicylic acid functionalized chitosan nanoparticle	0:50	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.	0:89	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.					
30389525	7	30	theme	commercial	1043:1052	arg1	biostimulant					1082:1093	a biostimulant	1080:1093	a biostimulant for plant disease control and higher yield	1080:1136	The obtained results claim commercial potential of SA-CS NPs as a biostimulant for plant disease control and higher yield.					
30389525	7	30	theme	commercial	1043:1052	arg1	potential					1054:1062	commercial potential	1043:1062	commercial potential of SA-CS NPs	1043:1075	The obtained results claim commercial potential of SA-CS NPs as a biostimulant for plant disease control and higher yield.					
30389525	3	31	from	NPs	480:482	arg1	SA					466:467	SA	466:467	SA from SA-CS NPs	466:482	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	31	from	NPs	480:482	arg1	profile					455:461	release profile	447:461	release profile of SA from SA-CS NPs	447:482	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	31	from	NPs	480:482	arg1	method					416:421	a method	414:421	a method of SA-CS NPs synthesis	414:444	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	0	32	theme	functionalized	15:28	arg1	nanoparticle					39:50	Salicylic acid functionalized chitosan nanoparticle	0:50	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.	0:89	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.					
30389525	4	33	theme	SA-CS	559:563	arg1	NPs					565:567	SA-CS NPs	559:567	SA-CS NPs	559:567	Findings unveil that SA-CS NPs expressed significant physiological-biochemical responses in vitro and in vivo.					
30389525	6	34	theme	59.4	868:871	arg1	%					872:872	%	872:872	%	872:872	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	3	35	theme	antifungal	485:494	arg1	activities					526:535	antifungal and seedling growth promoting activities	485:535	antifungal and seedling growth promoting activities	485:535	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	35	theme	antifungal	485:494	arg1	method					416:421	a method	414:421	a method of SA-CS NPs synthesis	414:444	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	1	36	theme	SA-CS	144:148	arg1	nanoparticles					129:141	salicylic acid-chitosan nanoparticles	105:141	salicylic acid-chitosan nanoparticles (SA-CS NPs)	105:153	In this work, salicylic acid-chitosan nanoparticles (SA-CS NPs) are reported as a biostimulant for promoting plant defense and growth in maize.					
30389525	1	36	theme	SA-CS	144:148	arg1	NPs					150:152	SA-CS NPs	144:152	SA-CS NPs	144:152	In this work, salicylic acid-chitosan nanoparticles (SA-CS NPs) are reported as a biostimulant for promoting plant defense and growth in maize.					
30389525	3	37	dep	promoting	516:524	arg1	growth					509:514	growth	509:514	growth	509:514	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	37	dep	promoting	516:524	arg1	seedling					500:507	seedling	500:507	seedling	500:507	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	5	38	theme	reactive	738:745	arg1	ROS					763:765	ROS	763:765	ROS	763:765	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	38	theme	reactive	738:745	arg1	control					823:829	disease control	815:829	disease control	815:829	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	38	theme	reactive	738:745	arg1	species					754:760	reactive oxygen species	738:760	reactive oxygen species (ROS)	738:766	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	38	theme	reactive	738:745	arg1	growth					841:846	plant growth	835:846	plant growth	835:846	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	38	theme	reactive	738:745	arg1	reinforcement					779:791	cell wall reinforcement	769:791	cell wall reinforcement by lignin deposition	769:812	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	6	39	theme	%	933:933	arg1	enhancement					941:951	57.8% yield enhancement	929:951	57.8% yield enhancement	929:951	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	1	40	theme	plant	200:204	arg1	defense					206:212	plant defense	200:212	plant defense	200:212	In this work, salicylic acid-chitosan nanoparticles (SA-CS NPs) are reported as a biostimulant for promoting plant defense and growth in maize.					
30389525	5	41	theme	lignin	796:801	arg1	deposition					803:812	lignin deposition	796:812	lignin deposition	796:812	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	6	42	theme	57.8	929:932	arg1	%					933:933	%	933:933	%	933:933	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	5	43	theme	oxygen	747:752	arg1	ROS					763:765	ROS	763:765	ROS	763:765	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	43	theme	oxygen	747:752	arg1	control					823:829	disease control	815:829	disease control	815:829	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	43	theme	oxygen	747:752	arg1	species					754:760	reactive oxygen species	738:760	reactive oxygen species (ROS)	738:766	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	43	theme	oxygen	747:752	arg1	growth					841:846	plant growth	835:846	plant growth	835:846	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	43	theme	oxygen	747:752	arg1	reinforcement					779:791	cell wall reinforcement	769:791	cell wall reinforcement by lignin deposition	769:812	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	3	44	theme	release	447:453	arg1	profile					455:461	release profile	447:461	release profile of SA from SA-CS NPs	447:482	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	44	theme	release	447:453	arg1	method					416:421	a method	414:421	a method of SA-CS NPs synthesis	414:444	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	0	45	theme	sustainable	55:65	arg1	biostimulant					67:78	A sustainable biostimulant	53:78	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.	0:89	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.					
30389525	5	46	theme	elevated	680:687	arg1	activities					716:725	elevated antioxidant-defense enzyme activities	680:725	elevated antioxidant-defense enzyme activities	680:725	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	46	theme	elevated	680:687	arg1	responses					653:661	The responses	649:661	The responses	649:661	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	1	47	theme	salicylic	105:113	arg1	nanoparticles					129:141	salicylic acid-chitosan nanoparticles	105:141	salicylic acid-chitosan nanoparticles (SA-CS NPs)	105:153	In this work, salicylic acid-chitosan nanoparticles (SA-CS NPs) are reported as a biostimulant for promoting plant defense and growth in maize.					
30389525	1	47	theme	salicylic	105:113	arg1	NPs					150:152	SA-CS NPs	144:152	SA-CS NPs	144:152	In this work, salicylic acid-chitosan nanoparticles (SA-CS NPs) are reported as a biostimulant for promoting plant defense and growth in maize.					
30389525	1	47	theme	salicylic	105:113	arg1	biostimulant					173:184	a biostimulant	171:184	a biostimulant for promoting plant defense and growth in maize	171:232	In this work, salicylic acid-chitosan nanoparticles (SA-CS NPs) are reported as a biostimulant for promoting plant defense and growth in maize.					
30389525	6	48	theme	disease	917:923	arg1	enhancement					941:951	57.8% yield enhancement	929:951	57.8% yield enhancement	929:951	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	6	48	theme	disease	917:923	arg1	control					874:880	59.4% control	868:880	59.4% control of post-flowering stalk rot (PFSR) disease	868:923	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	5	49	theme	antioxidant-defense	689:707	arg1	activities					716:725	elevated antioxidant-defense enzyme activities	680:725	elevated antioxidant-defense enzyme activities	680:725	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	49	theme	antioxidant-defense	689:707	arg1	responses					653:661	The responses	649:661	The responses	649:661	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	0	50	dep	nanoparticle	39:50	arg1	biostimulant					67:78	A sustainable biostimulant	53:78	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.	0:89	Salicylic acid functionalized chitosan nanoparticle: A sustainable biostimulant for plant.					
30389525	3	51	from	profile	455:461	arg1	NPs					480:482	SA-CS NPs	474:482	SA-CS NPs	474:482	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	1	52	theme	acid-chitosan	115:127	arg1	nanoparticles					129:141	salicylic acid-chitosan nanoparticles	105:141	salicylic acid-chitosan nanoparticles (SA-CS NPs)	105:153	In this work, salicylic acid-chitosan nanoparticles (SA-CS NPs) are reported as a biostimulant for promoting plant defense and growth in maize.					
30389525	1	52	theme	acid-chitosan	115:127	arg1	NPs					150:152	SA-CS NPs	144:152	SA-CS NPs	144:152	In this work, salicylic acid-chitosan nanoparticles (SA-CS NPs) are reported as a biostimulant for promoting plant defense and growth in maize.					
30389525	1	52	theme	acid-chitosan	115:127	arg1	biostimulant					173:184	a biostimulant	171:184	a biostimulant for promoting plant defense and growth in maize	171:232	In this work, salicylic acid-chitosan nanoparticles (SA-CS NPs) are reported as a biostimulant for promoting plant defense and growth in maize.					
30389525	7	53	theme	plant	1099:1103	arg1	control					1113:1119	plant disease control	1099:1119	plant disease control	1099:1119	The obtained results claim commercial potential of SA-CS NPs as a biostimulant for plant disease control and higher yield.					
30389525	5	54	theme	enzyme	709:714	arg1	activities					716:725	elevated antioxidant-defense enzyme activities	680:725	elevated antioxidant-defense enzyme activities	680:725	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	54	theme	enzyme	709:714	arg1	responses					653:661	The responses	649:661	The responses	649:661	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	2	55	theme	surface	315:321	arg1	chemistry					323:331	surface chemistry	315:331	surface chemistry	315:331	SA-CS NPs were characterised for colloidal size distribution, functional group, surface chemistry, chemical composition, crystal structure and morphology.					
30389525	6	56	theme	SA	1002:1003	arg1	treatment					1005:1013	SA treatment	1002:1013	SA treatment	1002:1013	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	3	57	theme	SA	466:467	arg1	profile					455:461	release profile	447:461	release profile of SA from SA-CS NPs	447:482	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	3	57	theme	SA	466:467	arg1	method					416:421	a method	414:421	a method of SA-CS NPs synthesis	414:444	Investigation discloses a method of SA-CS NPs synthesis, release profile of SA from SA-CS NPs, antifungal and seedling growth promoting activities.					
30389525	2	58	theme	SA-CS	235:239	arg1	NPs					241:243	SA-CS NPs	235:243	SA-CS NPs	235:243	SA-CS NPs were characterised for colloidal size distribution, functional group, surface chemistry, chemical composition, crystal structure and morphology.					
30389525	2	59	theme	crystal	356:362	arg1	structure					364:372	crystal structure	356:372	crystal structure	356:372	SA-CS NPs were characterised for colloidal size distribution, functional group, surface chemistry, chemical composition, crystal structure and morphology.					
30389525	5	60	theme	cell	769:772	arg1	species					754:760	reactive oxygen species	738:760	reactive oxygen species (ROS)	738:766	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	60	theme	cell	769:772	arg1	reinforcement					779:791	cell wall reinforcement	769:791	cell wall reinforcement by lignin deposition	769:812	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	6	61	theme	PFSR	911:914	arg1	disease					917:923	post-flowering stalk rot (PFSR) disease	885:923	post-flowering stalk rot (PFSR) disease	885:923	In field, 59.4% control of post-flowering stalk rot (PFSR) disease and 57.8% yield enhancement was evident in SA-CS NPs application compared to SA treatment.					
30389525	5	62	theme	plant	835:839	arg1	growth					841:846	plant growth	835:846	plant growth	835:846	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	62	theme	plant	835:839	arg1	species					754:760	reactive oxygen species	738:760	reactive oxygen species (ROS)	738:766	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	63	theme	disease	815:821	arg1	control					823:829	disease control	815:829	disease control	815:829	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30389525	5	63	theme	disease	815:821	arg1	species					754:760	reactive oxygen species	738:760	reactive oxygen species (ROS)	738:766	The responses were recorded as elevated antioxidant-defense enzyme activities, balancing reactive oxygen species (ROS), cell wall reinforcement by lignin deposition, disease control and plant growth in maize.					
30223028	6	0	theme	86:10:4	945:951	arg1	%					953:953	%	953:953	%	953:953	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	5	1	theme	loaded	864:869	arg1	cargo					871:875	the loaded cargo	860:875	the loaded cargo	860:875	LTSLs showed molecular weight dependent heat-triggered release of the loaded cargo.					
30223028	4	2	theme	varying	718:724	arg1	compositions					732:743	varying lipid compositions	718:743	varying lipid compositions	718:743	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	7	3	theme	released	1105:1112	arg1	ML1					1114:1116	released ML1	1105:1116	released ML1	1105:1116	Confocal live-cell imaging showed uptake of released ML1 after mild hyperthermia at 42 °C, subsequently leading to potent cytotoxicity by LTSL-ML1.					
30223028	7	4	theme	mild	1124:1127	arg1	hyperthermia					1129:1140	mild hyperthermia	1124:1140	mild hyperthermia at 42 °C	1124:1149	Confocal live-cell imaging showed uptake of released ML1 after mild hyperthermia at 42 °C, subsequently leading to potent cytotoxicity by LTSL-ML1.					
30223028	4	5	theme	hydration	762:770	arg1	method					772:777	the thin film hydration method	748:777	the thin film hydration method	748:777	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	4	6	theme	Model	620:624	arg1	macromolecules					626:639	Model macromolecules	620:639	Model macromolecules (dextrans, albumin)	620:659	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	1	7	used	used	183:186	arg2	liposomes					108:116	Lysolipid-containing thermosensitive liposomes	71:116	Lysolipid-containing thermosensitive liposomes (LTSL)	71:123	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	7	used	used	183:186	arg2	nanocarriers					154:165	clinically-relevant drug nanocarriers	129:165	clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release	129:311	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	8	theme	Lysolipid-containing	71:90	arg1	nanocarriers					154:165	clinically-relevant drug nanocarriers	129:165	clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release	129:311	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	8	theme	Lysolipid-containing	71:90	arg1	LTSL					119:122	LTSL	119:122	LTSL	119:122	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	8	theme	Lysolipid-containing	71:90	arg1	liposomes					108:116	Lysolipid-containing thermosensitive liposomes	71:116	Lysolipid-containing thermosensitive liposomes (LTSL)	71:123	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	3	9	theme	Mistletoe	514:522	arg1	ML1					534:536	ML1	534:536	ML1	534:536	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	3	9	theme	Mistletoe	514:522	arg1	lectin-1					524:531	Mistletoe lectin-1	514:531	Mistletoe lectin-1 (ML1)	514:537	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	5	10	theme	cargo	871:875	arg1	release					849:855	molecular weight dependent heat-triggered release	807:855	molecular weight dependent heat-triggered release of the loaded cargo	807:875	LTSLs showed molecular weight dependent heat-triggered release of the loaded cargo.					
30223028	2	11	link	plant-derived	457:469	arg1	cytotoxins					471:480	plant-derived cytotoxins	457:480	plant-derived cytotoxins	457:480	The objective of this study was to investigate the feasibility of LTSL for encapsulation and triggered release of macromolecular drugs such as plant-derived cytotoxins.					
30223028	1	12	theme	thermosensitive	92:106	arg1	nanocarriers					154:165	clinically-relevant drug nanocarriers	129:165	clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release	129:311	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	12	theme	thermosensitive	92:106	arg1	LTSL					119:122	LTSL	119:122	LTSL	119:122	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	12	theme	thermosensitive	92:106	arg1	liposomes					108:116	Lysolipid-containing thermosensitive liposomes	71:116	Lysolipid-containing thermosensitive liposomes (LTSL)	71:123	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	4	13	theme	small	690:694	arg1	LTSL					708:711	small unilamellar LTSL	690:711	small unilamellar LTSL with varying lipid compositions	690:743	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	7	14	theme	ML1	1114:1116	arg1	uptake					1095:1100	uptake	1095:1100	uptake of released ML1	1095:1116	Confocal live-cell imaging showed uptake of released ML1 after mild hyperthermia at 42 °C, subsequently leading to potent cytotoxicity by LTSL-ML1.					
30223028	5	15	theme	dependent	824:832	arg1	release					849:855	molecular weight dependent heat-triggered release	807:855	molecular weight dependent heat-triggered release of the loaded cargo	807:875	LTSLs showed molecular weight dependent heat-triggered release of the loaded cargo.					
30223028	4	16	with	LTSL	708:711	arg1	compositions					732:743	varying lipid compositions	718:743	varying lipid compositions	718:743	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	2	17	theme	plant-derived	457:469	arg1	cytotoxins					471:480	plant-derived cytotoxins	457:480	plant-derived cytotoxins	457:480	The objective of this study was to investigate the feasibility of LTSL for encapsulation and triggered release of macromolecular drugs such as plant-derived cytotoxins.					
30223028	1	18	theme	small	199:203	arg1	cytostatics					214:224	small molecule cytostatics	199:224	small molecule cytostatics	199:224	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	5	19	theme	heat-triggered	834:847	arg1	release					849:855	molecular weight dependent heat-triggered release	807:855	molecular weight dependent heat-triggered release of the loaded cargo	807:875	LTSLs showed molecular weight dependent heat-triggered release of the loaded cargo.					
30223028	4	20	theme	film	757:760	arg1	method					772:777	the thin film hydration method	748:777	the thin film hydration method	748:777	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	1	21	theme	molecule	205:212	arg1	cytostatics					214:224	small molecule cytostatics	199:224	small molecule cytostatics	199:224	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	0	22	theme	Thermosensitive	0:14	arg1	liposomes					16:24	Thermosensitive liposomes	0:24	Thermosensitive liposomes for	0:28	Thermosensitive liposomes for triggered release of cytotoxic proteins.					
30223028	1	23	theme	local	294:298	arg1	release					305:311	local drug release	294:311	local drug release	294:311	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	6	24	theme	mol	954:956	arg1	DPPC					958:961	86:10:4 %mol DPPC	945:961	86:10:4 %mol DPPC	945:961	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	4	25	theme	thin	752:755	arg1	film					757:760	the thin film	748:760	the thin film hydration method	748:777	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	7	26	theme	potent	1176:1181	arg1	cytotoxicity					1183:1194	potent cytotoxicity	1176:1194	potent cytotoxicity by LTSL-ML1	1176:1206	Confocal live-cell imaging showed uptake of released ML1 after mild hyperthermia at 42 °C, subsequently leading to potent cytotoxicity by LTSL-ML1.					
30223028	1	27	theme	drug	300:303	arg1	release					305:311	local drug release	294:311	local drug release	294:311	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	3	28	theme	potent	578:583	arg1	activity					595:602	potent cytotoxic activity	578:602	potent cytotoxic activity in tumor cells	578:617	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	2	29	theme	drugs	443:447	arg1	release					417:423	release	417:423	release of macromolecular drugs such as plant-derived cytotoxins	417:480	The objective of this study was to investigate the feasibility of LTSL for encapsulation and triggered release of macromolecular drugs such as plant-derived cytotoxins.					
30223028	1	30	with	combination	239:249	arg1	hyperthermia					262:273	local hyperthermia	256:273	local hyperthermia (42 °C)	256:281	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	30	with	combination	239:249	arg1	42 °C					276:280	42 °C	276:280	42 °C	276:280	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	3	31	theme	cytotoxic	585:593	arg1	activity					595:602	potent cytotoxic activity	578:602	potent cytotoxic activity in tumor cells	578:617	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	2	32	theme	macromolecular	428:441	arg1	cytotoxins					471:480	plant-derived cytotoxins	457:480	plant-derived cytotoxins	457:480	The objective of this study was to investigate the feasibility of LTSL for encapsulation and triggered release of macromolecular drugs such as plant-derived cytotoxins.					
30223028	2	32	theme	macromolecular	428:441	arg1	drugs					443:447	macromolecular drugs	428:447	macromolecular drugs such as plant-derived cytotoxins	428:480	The objective of this study was to investigate the feasibility of LTSL for encapsulation and triggered release of macromolecular drugs such as plant-derived cytotoxins.					
30223028	4	33	theme	lipid	726:730	arg1	compositions					732:743	varying lipid compositions	718:743	varying lipid compositions	718:743	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	7	34	theme	live-cell	1070:1078	arg1	imaging					1080:1086	Confocal live-cell imaging	1061:1086	Confocal live-cell imaging	1061:1086	Confocal live-cell imaging showed uptake of released ML1 after mild hyperthermia at 42 °C, subsequently leading to potent cytotoxicity by LTSL-ML1.					
30223028	3	35	used	used	509:512	arg2	we					506:507	we	506:507	we	506:507	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	6	36	dep	ML1	910:912	arg1	DSPE-PEG2000					968:979	DSPE-PEG2000	968:979	DSPE-PEG2000	968:979	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	6	36	dep	ML1	910:912	arg1	MSPC					963:966	MSPC	963:966	ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000	910:979	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	8	37	theme	tumor	1300:1304	arg1	strategy					1315:1322	local tumor delivery strategy	1294:1322	local tumor delivery strategy for macromolecular cytotoxins	1294:1352	Our study shows that LTSL in combination with localized hyperthermia hold promise as local tumor delivery strategy for macromolecular cytotoxins.					
30223028	8	38	theme	localized	1255:1263	arg1	hyperthermia					1265:1276	localized hyperthermia	1255:1276	localized hyperthermia	1255:1276	Our study shows that LTSL in combination with localized hyperthermia hold promise as local tumor delivery strategy for macromolecular cytotoxins.					
30223028	6	39	theme	promising	887:895	arg1	ML1					910:912	ML1	910:912	ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000	910:979	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	6	39	theme	promising	887:895	arg1	composition					897:907	The most promising composition	878:907	The most promising composition	878:907	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	6	40	theme	carcinoma	1044:1052	arg1	cells					1054:1058	murine CT26 colon carcinoma cells	1026:1058	murine CT26 colon carcinoma cells	1026:1058	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	0	41	theme	proteins	61:68	arg1	release					40:46	release	40:46	release of cytotoxic proteins	40:68	Thermosensitive liposomes for triggered release of cytotoxic proteins.					
30223028	7	42	from	42 °C	1145:1149	arg1	hyperthermia					1129:1140	mild hyperthermia	1124:1140	mild hyperthermia at 42 °C	1124:1149	Confocal live-cell imaging showed uptake of released ML1 after mild hyperthermia at 42 °C, subsequently leading to potent cytotoxicity by LTSL-ML1.					
30223028	3	43	theme	tumor	607:611	arg1	cells					613:617	tumor cells	607:617	tumor cells	607:617	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	8	44	from	LTSL	1230:1233	arg1	combination					1238:1248	combination	1238:1248	combination with localized hyperthermia	1238:1276	Our study shows that LTSL in combination with localized hyperthermia hold promise as local tumor delivery strategy for macromolecular cytotoxins.					
30223028	1	45	theme	clinically-relevant	129:147	arg1	nanocarriers					154:165	clinically-relevant drug nanocarriers	129:165	clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release	129:311	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	45	theme	clinically-relevant	129:147	arg1	liposomes					108:116	Lysolipid-containing thermosensitive liposomes	71:116	Lysolipid-containing thermosensitive liposomes (LTSL)	71:123	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	0	46	theme	cytotoxic	51:59	arg1	proteins					61:68	cytotoxic proteins	51:68	cytotoxic proteins	51:68	Thermosensitive liposomes for triggered release of cytotoxic proteins.					
30223028	7	47	theme	Confocal	1061:1068	arg1	imaging					1080:1086	Confocal live-cell imaging	1061:1086	Confocal live-cell imaging	1061:1086	Confocal live-cell imaging showed uptake of released ML1 after mild hyperthermia at 42 °C, subsequently leading to potent cytotoxicity by LTSL-ML1.					
30223028	1	48	theme	drug	149:152	arg1	nanocarriers					154:165	clinically-relevant drug nanocarriers	129:165	clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release	129:311	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	48	theme	drug	149:152	arg1	liposomes					108:116	Lysolipid-containing thermosensitive liposomes	71:116	Lysolipid-containing thermosensitive liposomes (LTSL)	71:123	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	6	49	theme	CT26	1033:1036	arg1	cells					1054:1058	murine CT26 colon carcinoma cells	1026:1058	murine CT26 colon carcinoma cells	1026:1058	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	1	50	from	tumors	229:234	arg1	combination					239:249	combination	239:249	combination with local hyperthermia (42 °C) to trigger local drug release	239:311	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	8	51	theme	macromolecular	1328:1341	arg1	cytotoxins					1343:1352	macromolecular cytotoxins	1328:1352	macromolecular cytotoxins	1328:1352	Our study shows that LTSL in combination with localized hyperthermia hold promise as local tumor delivery strategy for macromolecular cytotoxins.					
30223028	6	52	theme	colon	1038:1042	arg1	cells					1054:1058	murine CT26 colon carcinoma cells	1026:1058	murine CT26 colon carcinoma cells	1026:1058	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	3	53	theme	ribosome-inactivating	543:563	arg1	protein					565:571	a ribosome-inactivating protein	541:571	a ribosome-inactivating protein with potent cytotoxic activity in tumor cells	541:617	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	8	54	with	combination	1238:1248	arg1	hyperthermia					1265:1276	localized hyperthermia	1255:1276	localized hyperthermia	1255:1276	Our study shows that LTSL in combination with localized hyperthermia hold promise as local tumor delivery strategy for macromolecular cytotoxins.					
30223028	2	55	theme	study	336:340	arg1	objective					318:326	The objective	314:326	The objective of this study	314:340	The objective of this study was to investigate the feasibility of LTSL for encapsulation and triggered release of macromolecular drugs such as plant-derived cytotoxins.					
30223028	3	56	from	activity	595:602	arg1	cells					613:617	tumor cells	607:617	tumor cells	607:617	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	1	57	theme	local	256:260	arg1	hyperthermia					262:273	local hyperthermia	256:273	local hyperthermia (42 °C)	256:281	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	1	57	theme	local	256:260	arg1	42 °C					276:280	42 °C	276:280	42 °C	276:280	Lysolipid-containing thermosensitive liposomes (LTSL) are clinically-relevant drug nanocarriers which have been used to deliver small molecule cytostatics to tumors in combination with local hyperthermia (42 °C) to trigger local drug release.					
30223028	4	58	dep	macromolecules	626:639	arg1	albumin					652:658	albumin	652:658	albumin	652:658	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	4	58	dep	macromolecules	626:639	arg1	dextrans					642:649	dextrans	642:649	dextrans	642:649	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	8	59	theme	local	1294:1298	arg1	strategy					1315:1322	local tumor delivery strategy	1294:1322	local tumor delivery strategy for macromolecular cytotoxins	1294:1352	Our study shows that LTSL in combination with localized hyperthermia hold promise as local tumor delivery strategy for macromolecular cytotoxins.					
30223028	3	60	theme	therapeutic	486:496	arg1	protein					498:504	therapeutic protein	486:504	therapeutic protein	486:504	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	2	61	theme	LTSL	380:383	arg1	feasibility					365:375	the feasibility	361:375	the feasibility of LTSL for encapsulation	361:401	The objective of this study was to investigate the feasibility of LTSL for encapsulation and triggered release of macromolecular drugs such as plant-derived cytotoxins.					
30223028	6	62	theme	murine	1026:1031	arg1	cells					1054:1058	murine CT26 colon carcinoma cells	1026:1058	murine CT26 colon carcinoma cells	1026:1058	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	3	63	with	protein	565:571	arg1	activity					595:602	potent cytotoxic activity	578:602	potent cytotoxic activity in tumor cells	578:617	As therapeutic protein we used Mistletoe lectin-1 (ML1) - a ribosome-inactivating protein with potent cytotoxic activity in tumor cells.					
30223028	6	64	theme	%	953:953	arg1	DPPC					958:961	86:10:4 %mol DPPC	945:961	86:10:4 %mol DPPC	945:961	The most promising composition, ML1 formulated in LTSL composed of 86:10:4 %mol DPPC:MSPC:DSPE-PEG2000, was further studied for bioactivity against murine CT26 colon carcinoma cells.					
30223028	4	65	theme	unilamellar	696:706	arg1	LTSL					708:711	small unilamellar LTSL	690:711	small unilamellar LTSL with varying lipid compositions	690:743	Model macromolecules (dextrans, albumin) and ML1 were encapsulated in small unilamellar LTSL with varying lipid compositions by the thin film hydration method and extrusion.					
30223028	8	66	theme	delivery	1306:1313	arg1	strategy					1315:1322	local tumor delivery strategy	1294:1322	local tumor delivery strategy for macromolecular cytotoxins	1294:1352	Our study shows that LTSL in combination with localized hyperthermia hold promise as local tumor delivery strategy for macromolecular cytotoxins.					
31887374	0	0	theme	aqueous	93:99	arg1	solution					101:108	aqueous solution	93:108	aqueous solution	93:108	Chitosan/Al2O3-HA nanocomposite beads for efficient removal of estradiol and chrysoidin from aqueous solution.					
31887374	2	1	theme	thermogravimetric	477:493	arg1	analysis					495:502	thermogravimetric analysis	477:502	thermogravimetric analysis (TGA)	477:508	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	2	1	theme	thermogravimetric	477:493	arg1	TGA					505:507	TGA	505:507	TGA	505:507	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	4	2	theme	batch	855:859	arg1	experiments					861:871	the batch experiments	851:871	the batch experiments	851:871	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	6	3	theme	adsorption	1006:1015	arg1	39.78 mg/g					1042:1051	39.78 mg/g	1042:1051	39.78 mg/g	1042:1051	The maximum adsorption capacity was found to be 39.78 mg/g and 23.26 mg/g for estradiol and chrysoidine, respectively.					
31887374	6	3	theme	adsorption	1006:1015	arg1	capacity					1017:1024	The maximum adsorption capacity	994:1024	The maximum adsorption capacity	994:1024	The maximum adsorption capacity was found to be 39.78 mg/g and 23.26 mg/g for estradiol and chrysoidine, respectively.					
31887374	7	4	theme	H2SO4/MeOH	1224:1233	arg1	eluent					1208:1213	the eluent	1204:1213	the eluent of 0.1 M H2SO4/MeOH	1204:1233	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	2	5	dep	Spectrometry	456:467	arg1	Infrared					447:454	Infrared	447:454	Infrared	447:454	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	2	5	dep	Spectrometry	456:467	arg1	FTIR					470:473	FTIR	470:473	FTIR	470:473	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	6	6	theme	maximum	998:1004	arg1	39.78 mg/g					1042:1051	39.78 mg/g	1042:1051	39.78 mg/g	1042:1051	The maximum adsorption capacity was found to be 39.78 mg/g and 23.26 mg/g for estradiol and chrysoidine, respectively.					
31887374	6	6	theme	maximum	998:1004	arg1	capacity					1017:1024	The maximum adsorption capacity	994:1024	The maximum adsorption capacity	994:1024	The maximum adsorption capacity was found to be 39.78 mg/g and 23.26 mg/g for estradiol and chrysoidine, respectively.					
31887374	2	7	theme	Brunauer-Emmett-Teller	514:535	arg1	analysis					556:563	Brunauer-Emmett-Teller (BET) surface area analysis	514:563	Brunauer-Emmett-Teller (BET) surface area analysis	514:563	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	7	8	theme	adsorbed	1117:1124	arg1	estradiol					1126:1134	The adsorbed estradiol	1113:1134	The adsorbed estradiol	1113:1134	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	5	9	theme	described	959:967	arg1	model					948:952	the pseudo-second-order model	924:952	the pseudo-second-order model best described	924:967	Isotherm data best matched the Langmuir model and the pseudo-second-order model best described the adsorption kinetics.					
31887374	7	10	theme	0.1 M	1218:1222	arg1	H2SO4/MeOH					1224:1233	0.1 M H2SO4/MeOH	1218:1233	0.1 M H2SO4/MeOH	1218:1233	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	7	11	with	beads	1193:1197	arg1	eluent					1208:1213	the eluent	1204:1213	the eluent of 0.1 M H2SO4/MeOH	1204:1233	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	1	12	theme	chrysoidin	269:278	arg1	removal					280:286	chrysoidin removal	269:286	chrysoidin removal from aqueous solution	269:308	Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work.					
31887374	8	13	theme	efficient	1425:1433	arg1	removal					1435:1441	the efficient removal estradiol and chrysoidin	1421:1466	removal	1435:1441	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	2	14	dep	Fourier	429:435	arg1	Transform					437:445	Transform	437:445	Transform Infrared Spectrometry (FTIR)	437:474	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	5	15	theme	adsorption	973:982	arg1	kinetics					984:991	the adsorption kinetics	969:991	the adsorption kinetics	969:991	Isotherm data best matched the Langmuir model and the pseudo-second-order model best described the adsorption kinetics.					
31887374	2	16	theme	Electron	402:409	arg1	SEM					423:425	SEM	423:425	SEM	423:425	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	2	16	theme	Electron	402:409	arg1	Microscopy					411:420	Scanning Electron Microscopy	393:420	Scanning Electron Microscopy (SEM)	393:426	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	1	17	from	solution	301:308	arg1	estradiol					255:263	estradiol	255:263	estradiol	255:263	Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work.					
31887374	1	17	from	solution	301:308	arg1	removal					280:286	chrysoidin removal	269:286	chrysoidin removal from aqueous solution	269:308	Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work.					
31887374	8	18	theme	developed	1371:1379	arg1	beads					1391:1395	the developed composite beads	1367:1395	the developed composite beads	1367:1395	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	2	19	theme	Scanning	393:400	arg1	SEM					423:425	SEM	423:425	SEM	423:425	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	2	19	theme	Scanning	393:400	arg1	Microscopy					411:420	Scanning Electron Microscopy	393:420	Scanning Electron Microscopy (SEM)	393:426	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	3	20	theme	beads	707:711	arg1	surface					692:698	the surface	688:698	the surface of the beads after the synthesis	688:731	FTIR spectra confirmed that the chitosan was loaded successfully on Al2O3-HA, and functional groups were immobilized onto the surface of the beads after the synthesis.					
31887374	8	21	contain	have	1397:1400	arg2	potential					1407:1415	high potential	1402:1415	high potential for the efficient removal estradiol and chrysoidin from aqueous solution	1402:1488	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	8	21	contain	have	1397:1400	arg1	beads					1391:1395	the developed composite beads	1367:1395	the developed composite beads	1367:1395	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	3	22	theme	FTIR	566:569	arg1	spectra					571:577	FTIR spectra	566:577	FTIR spectra	566:577	FTIR spectra confirmed that the chitosan was loaded successfully on Al2O3-HA, and functional groups were immobilized onto the surface of the beads after the synthesis.					
31887374	2	23	theme	surface	543:549	arg1	analysis					556:563	Brunauer-Emmett-Teller (BET) surface area analysis	514:563	Brunauer-Emmett-Teller (BET) surface area analysis	514:563	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	7	24	from	deterioration	1299:1311	arg1	performances					1328:1339	its initial performances	1316:1339	its initial performances	1316:1339	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	4	25	theme	adsorption	738:747	arg1	condition					749:757	The adsorption condition	734:757	The adsorption condition	734:757	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	7	26	theme	initial	1320:1326	arg1	performances					1328:1339	its initial performances	1316:1339	its initial performances	1316:1339	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	5	27	theme	pseudo-second-order	928:946	arg1	model					948:952	the pseudo-second-order model	924:952	the pseudo-second-order model best described	924:967	Isotherm data best matched the Langmuir model and the pseudo-second-order model best described the adsorption kinetics.					
31887374	1	28	theme	aqueous	293:299	arg1	solution					301:308	aqueous solution	293:308	aqueous solution	293:308	Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work.					
31887374	8	29	theme	composite	1381:1389	arg1	beads					1391:1395	the developed composite beads	1367:1395	the developed composite beads	1367:1395	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	8	30	theme	high	1402:1405	arg1	potential					1407:1415	high potential	1402:1415	high potential for the efficient removal estradiol and chrysoidin from aqueous solution	1402:1488	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	8	31	dep	removal	1435:1441	arg1	estradiol					1443:1451	estradiol	1443:1451	estradiol	1443:1451	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	5	32	theme	Langmuir	905:912	arg1	model					914:918	the Langmuir model	901:918	the Langmuir model	901:918	Isotherm data best matched the Langmuir model and the pseudo-second-order model best described the adsorption kinetics.					
31887374	2	33	theme	BET	538:540	arg1	analysis					556:563	Brunauer-Emmett-Teller (BET) surface area analysis	514:563	Brunauer-Emmett-Teller (BET) surface area analysis	514:563	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	8	34	theme	aqueous	1473:1479	arg1	solution					1481:1488	aqueous solution	1473:1488	aqueous solution	1473:1488	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	1	35	theme	chitosan/Al2O3-HA	204:220	arg1	beads					232:236	chitosan/Al2O3-HA composite beads	204:236	chitosan/Al2O3-HA composite beads	204:236	Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work.					
31887374	0	36	theme	efficient	42:50	arg1	removal					52:58	efficient removal	42:58	efficient removal of estradiol and chrysoidin from aqueous solution	42:108	Chitosan/Al2O3-HA nanocomposite beads for efficient removal of estradiol and chrysoidin from aqueous solution.					
31887374	1	37	theme	composite	222:230	arg1	beads					232:236	chitosan/Al2O3-HA composite beads	204:236	chitosan/Al2O3-HA composite beads	204:236	Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work.					
31887374	0	38	theme	estradiol	63:71	arg1	removal					52:58	efficient removal	42:58	efficient removal of estradiol and chrysoidin from aqueous solution	42:108	Chitosan/Al2O3-HA nanocomposite beads for efficient removal of estradiol and chrysoidin from aqueous solution.					
31887374	4	39	dep	adsorbent	787:795	arg1	initial					798:804	initial	798:804	initial	798:804	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	1	40	theme	present	317:323	arg1	work					325:328	the present work	313:328	the present work	313:328	Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work.					
31887374	1	41	used	used	246:249	arg2	Alumina					111:117	Alumina	111:117	Alumina	111:117	Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work.					
31887374	4	42	theme	concentration	806:818	arg1	time					824:827	time	824:827	time	824:827	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	4	42	theme	concentration	806:818	arg1	concentration					806:818	concentration	806:818	concentration	806:818	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	4	42	theme	concentration	806:818	arg1	amount					777:782	the amount	773:782	the amount of adsorbent, initial concentration and time	773:827	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	7	43	theme	composite	1183:1191	arg1	beads					1193:1197	the composite beads	1179:1197	the composite beads with the eluent of 0.1 M H2SO4/MeOH	1179:1233	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	2	44	theme	physicochemical	335:349	arg1	properties					351:360	The physicochemical properties	331:360	The physicochemical properties of the beads	331:373	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	8	45	from	solution	1481:1488	arg1	removal					1435:1441	the efficient removal estradiol and chrysoidin	1421:1466	removal	1435:1441	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	8	45	from	solution	1481:1488	arg1	chrysoidin					1457:1466	chrysoidin	1457:1466	chrysoidin	1457:1466	This study suggests that the developed composite beads have high potential for the efficient removal estradiol and chrysoidin from aqueous solution.					
31887374	1	46	theme	support	125:131	arg1	material					133:140	a support material	123:140	a support material	123:140	Alumina, as a support material, was loaded together with chitosan and hydroxyapatite to form chitosan/Al2O3-HA composite beads and was used for estradiol and chrysoidin removal from aqueous solution in the present work.					
31887374	4	47	theme	time	824:827	arg1	time					824:827	time	824:827	time	824:827	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	4	47	theme	time	824:827	arg1	concentration					806:818	concentration	806:818	concentration	806:818	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	4	47	theme	time	824:827	arg1	amount					777:782	the amount	773:782	the amount of adsorbent, initial concentration and time	773:827	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	2	48	theme	area	551:554	arg1	analysis					556:563	Brunauer-Emmett-Teller (BET) surface area analysis	514:563	Brunauer-Emmett-Teller (BET) surface area analysis	514:563	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31887374	3	49	theme	functional	648:657	arg1	groups					659:664	functional groups	648:664	functional groups	648:664	FTIR spectra confirmed that the chitosan was loaded successfully on Al2O3-HA, and functional groups were immobilized onto the surface of the beads after the synthesis.					
31887374	5	50	dep	model	914:918	arg1	kinetics					984:991	the adsorption kinetics	969:991	the adsorption kinetics	969:991	Isotherm data best matched the Langmuir model and the pseudo-second-order model best described the adsorption kinetics.					
31887374	7	51	theme	several	1276:1282	arg1	cycles					1284:1289	several cycles	1276:1289	several cycles without deterioration in its initial performances	1276:1339	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	0	52	theme	chrysoidin	77:86	arg1	removal					52:58	efficient removal	42:58	efficient removal of estradiol and chrysoidin from aqueous solution	42:108	Chitosan/Al2O3-HA nanocomposite beads for efficient removal of estradiol and chrysoidin from aqueous solution.					
31887374	0	53	from	solution	101:108	arg1	removal					52:58	efficient removal	42:58	efficient removal of estradiol and chrysoidin from aqueous solution	42:108	Chitosan/Al2O3-HA nanocomposite beads for efficient removal of estradiol and chrysoidin from aqueous solution.					
31887374	4	54	theme	adsorbent	787:795	arg1	concentration					806:818	concentration	806:818	concentration	806:818	The adsorption condition including pH, the amount of adsorbent, initial concentration and time were evaluated during the batch experiments.					
31887374	5	55	theme	Isotherm	874:881	arg1	data					883:886	Isotherm data	874:886	Isotherm data	874:886	Isotherm data best matched the Langmuir model and the pseudo-second-order model best described the adsorption kinetics.					
31887374	7	56	theme	regenerated	1243:1253	arg1	material					1255:1262	the regenerated material	1239:1262	the regenerated material	1239:1262	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	7	57	used	used	1268:1271	arg2	material					1255:1262	the regenerated material	1239:1262	the regenerated material	1239:1262	The adsorbed estradiol and chrysoidin were completely eluted from the composite beads with the eluent of 0.1 M H2SO4/MeOH and the regenerated material was used in several cycles without deterioration in its initial performances.					
31887374	2	58	theme	beads	369:373	arg1	properties					351:360	The physicochemical properties	331:360	The physicochemical properties of the beads	331:373	The physicochemical properties of the beads were studied with Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrometry (FTIR), thermogravimetric analysis (TGA) and Brunauer-Emmett-Teller (BET) surface area analysis.					
31082187	8	0	theme	clinical	1272:1279	arg1	therapy					1288:1294	clinical cancer therapy	1272:1294	clinical cancer therapy	1272:1294	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	4	1	theme	composite	614:622	arg1	nanoparticles					624:636	The obtained composite nanoparticles	601:636	The obtained composite nanoparticles	601:636	The obtained composite nanoparticles show a homogeneous size distribution with good dispersity and pH sensitivity.					
31082187	8	2	theme	powerful	1145:1152	arg1	method					1154:1159	a powerful method	1143:1159	a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy	1143:1294	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	8	2	theme	powerful	1145:1152	arg1	strategy					1119:1126	This new strategy	1110:1126	This new strategy	1110:1126	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	6	3	theme	DOX	861:863	arg1	result					822:827	a result	820:827	a result	820:827	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	3	theme	DOX	861:863	arg1	%					931:931	17.3%	927:931	17.3% of the injected dosage	927:954	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	3	theme	DOX	861:863	arg1	retention					848:856	the intracellular retention	830:856	the intracellular retention of DOX	830:863	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	3	theme	DOX	861:863	arg1	dosage					949:954	the injected dosage	936:954	the injected dosage	936:954	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	8	4	from	application	1240:1250	arg1	therapy					1288:1294	clinical cancer therapy	1272:1294	clinical cancer therapy	1272:1294	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	0	5	theme	Cancer	124:129	arg1	Therapy					131:137	Effective Cancer Therapy	114:137	Effective Cancer Therapy	114:137	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.					
31082187	8	6	from	therapy	1288:1294	arg1	application					1240:1250	the application	1236:1250	the application of nanoparticles in clinical cancer therapy	1236:1294	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	7	7	theme	combined	986:993	arg1	strategy					995:1002	this combined strategy	981:1002	this combined strategy	981:1002	The antitumor effect of this combined strategy was remarkably improved in both the DOX-sensitive HepG2 and DOX-resistant HepG2/ADR tumor models in vivo.					
31082187	3	8	theme	self-assembled	430:443	arg1	nanocarriers					476:487	nanocarriers	476:487	nanocarriers	476:487	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	3	8	theme	self-assembled	430:443	arg1	nanoparticles					445:457	pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles	388:457	pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles	388:457	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	7	9	theme	DOX-resistant	1064:1076	arg1	models					1094:1099	DOX-resistant HepG2/ADR tumor models	1064:1099	DOX-resistant HepG2/ADR tumor models in vivo	1064:1107	The antitumor effect of this combined strategy was remarkably improved in both the DOX-sensitive HepG2 and DOX-resistant HepG2/ADR tumor models in vivo.					
31082187	5	10	theme	nanoparticles	802:814	arg1	penetration					781:791	the intratumoral penetration	764:791	the intratumoral penetration of these nanoparticles	764:814	Furthermore, ultrasound was utilized to promote the intratumoral penetration of these nanoparticles.					
31082187	7	11	theme	in	1101:1102	arg1	models					1094:1099	DOX-resistant HepG2/ADR tumor models	1064:1099	DOX-resistant HepG2/ADR tumor models in vivo	1064:1107	The antitumor effect of this combined strategy was remarkably improved in both the DOX-sensitive HepG2 and DOX-resistant HepG2/ADR tumor models in vivo.					
31082187	7	12	theme	strategy	995:1002	arg1	effect					971:976	The antitumor effect	957:976	The antitumor effect of this combined strategy	957:1002	The antitumor effect of this combined strategy was remarkably improved in both the DOX-sensitive HepG2 and DOX-resistant HepG2/ADR tumor models in vivo.					
31082187	8	13	theme	cancer	1281:1286	arg1	therapy					1288:1294	clinical cancer therapy	1272:1294	clinical cancer therapy	1272:1294	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	0	14	from	Nanoparticles	96:108	arg1	Delivery					20:27	Ultrasound-Enhanced Delivery	0:27	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.	0:138	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.					
31082187	7	15	theme	HepG2/ADR	1078:1086	arg1	models					1094:1099	DOX-resistant HepG2/ADR tumor models	1064:1099	DOX-resistant HepG2/ADR tumor models in vivo	1064:1107	The antitumor effect of this combined strategy was remarkably improved in both the DOX-sensitive HepG2 and DOX-resistant HepG2/ADR tumor models in vivo.					
31082187	2	16	theme	vascular	338:345	arg1	penetration					347:357	insufficient tumor vascular penetration	319:357	insufficient tumor vascular penetration	319:357	However, its clinical application is severely limited because of insufficient tumor vascular penetration.					
31082187	3	17	theme	pullulan-ATR	416:427	arg1	nanocarriers					476:487	nanocarriers	476:487	nanocarriers	476:487	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	3	17	theme	pullulan-ATR	416:427	arg1	nanoparticles					445:457	pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles	388:457	pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles	388:457	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	7	18	theme	tumor	1088:1092	arg1	models					1094:1099	DOX-resistant HepG2/ADR tumor models	1064:1099	DOX-resistant HepG2/ADR tumor models in vivo	1064:1107	The antitumor effect of this combined strategy was remarkably improved in both the DOX-sensitive HepG2 and DOX-resistant HepG2/ADR tumor models in vivo.					
31082187	2	19	theme	tumor	332:336	arg1	penetration					347:357	insufficient tumor vascular penetration	319:357	insufficient tumor vascular penetration	319:357	However, its clinical application is severely limited because of insufficient tumor vascular penetration.					
31082187	1	20	theme	cancer	208:213	arg1	therapy					215:221	cancer therapy	208:221	cancer therapy	208:221	Nanodiamond as a drug carrier is of great significance in improving cancer therapy by overcoming chemoresistance.					
31082187	4	21	theme	obtained	605:612	arg1	nanoparticles					624:636	The obtained composite nanoparticles	601:636	The obtained composite nanoparticles	601:636	The obtained composite nanoparticles show a homogeneous size distribution with good dispersity and pH sensitivity.					
31082187	2	22	theme	insufficient	319:330	arg1	penetration					347:357	insufficient tumor vascular penetration	319:357	insufficient tumor vascular penetration	319:357	However, its clinical application is severely limited because of insufficient tumor vascular penetration.					
31082187	6	23	theme	intracellular	834:846	arg1	result					822:827	a result	820:827	a result	820:827	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	23	theme	intracellular	834:846	arg1	%					931:931	17.3%	927:931	17.3% of the injected dosage	927:954	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	23	theme	intracellular	834:846	arg1	retention					848:856	the intracellular retention	830:856	the intracellular retention of DOX	830:863	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	23	theme	intracellular	834:846	arg1	dosage					949:954	the injected dosage	936:954	the injected dosage	936:954	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	5	24	theme	intratumoral	768:779	arg1	penetration					781:791	the intratumoral penetration	764:791	the intratumoral penetration of these nanoparticles	764:814	Furthermore, ultrasound was utilized to promote the intratumoral penetration of these nanoparticles.					
31082187	3	25	theme	pullulan-all-trans-retinal	388:413	arg1	nanocarriers					476:487	nanocarriers	476:487	nanocarriers	476:487	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	3	25	theme	pullulan-all-trans-retinal	388:413	arg1	nanoparticles					445:457	pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles	388:457	pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles	388:457	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	0	26	theme	Ultrasound-Enhanced	0:18	arg1	Delivery					20:27	Ultrasound-Enhanced Delivery	0:27	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.	0:138	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.					
31082187	6	27	theme	tumor	906:910	arg1	tissue					912:917	the tumor tissue	902:917	the tumor tissue	902:917	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	4	28	with	distribution	662:673	arg1	dispersity					685:694	good dispersity	680:694	good dispersity	680:694	The obtained composite nanoparticles show a homogeneous size distribution with good dispersity and pH sensitivity.					
31082187	4	28	with	distribution	662:673	arg1	sensitivity					703:713	pH sensitivity	700:713	pH sensitivity	700:713	The obtained composite nanoparticles show a homogeneous size distribution with good dispersity and pH sensitivity.					
31082187	0	29	theme	Doxorubicin-Loaded	32:49	arg1	Nanodiamonds					51:62	Doxorubicin-Loaded Nanodiamonds	32:62	Doxorubicin-Loaded Nanodiamonds	32:62	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.					
31082187	4	30	theme	good	680:683	arg1	dispersity					685:694	good dispersity	680:694	good dispersity	680:694	The obtained composite nanoparticles show a homogeneous size distribution with good dispersity and pH sensitivity.					
31082187	5	31	used	utilized	744:751	arg2	ultrasound					729:738	ultrasound	729:738	ultrasound	729:738	Furthermore, ultrasound was utilized to promote the intratumoral penetration of these nanoparticles.					
31082187	3	32	theme	structured	568:577	arg1	nanosystem					589:598	a core-shell structured coloading nanosystem	555:598	a core-shell structured coloading nanosystem	555:598	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	8	33	theme	nanoparticles	1255:1267	arg1	application					1240:1250	the application	1236:1250	the application of nanoparticles in clinical cancer therapy	1236:1294	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	4	34	theme	pH	700:701	arg1	sensitivity					703:713	pH sensitivity	700:713	pH sensitivity	700:713	The obtained composite nanoparticles show a homogeneous size distribution with good dispersity and pH sensitivity.					
31082187	8	35	theme	nanodiamonds	1190:1201	arg1	limitation					1176:1185	the limitation	1172:1185	the limitation of nanodiamonds	1172:1201	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	3	36	theme	coloading	579:587	arg1	nanosystem					589:598	a core-shell structured coloading nanosystem	555:598	a core-shell structured coloading nanosystem	555:598	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	8	37	theme	innovative	1215:1224	arg1	ideas					1226:1230	innovative ideas	1215:1230	innovative ideas for the application of nanoparticles in clinical cancer therapy	1215:1294	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	0	38	theme	Nanodiamonds	51:62	arg1	Delivery					20:27	Ultrasound-Enhanced Delivery	0:27	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.	0:138	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.					
31082187	4	39	theme	size	657:660	arg1	distribution					662:673	a homogeneous size distribution	643:673	a homogeneous size distribution with good dispersity and pH sensitivity	643:713	The obtained composite nanoparticles show a homogeneous size distribution with good dispersity and pH sensitivity.					
31082187	3	40	theme	doxorubicin-loaded	509:526	arg1	nanodiamonds					528:539	doxorubicin-loaded nanodiamonds	509:539	doxorubicin-loaded nanodiamonds	509:539	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	8	41	from	nanoparticles	1255:1267	arg1	therapy					1288:1294	clinical cancer therapy	1272:1294	clinical cancer therapy	1272:1294	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	3	42	theme	core-shell	557:566	arg1	nanosystem					589:598	a core-shell structured coloading nanosystem	555:598	a core-shell structured coloading nanosystem	555:598	To address this limitation, pullulan-all-trans-retinal (pullulan-ATR) self-assembled nanoparticles were prepared as nanocarriers, which encapsulated doxorubicin-loaded nanodiamonds, to construct a core-shell structured coloading nanosystem.					
31082187	4	43	theme	homogeneous	645:655	arg1	distribution					662:673	a homogeneous size distribution	643:673	a homogeneous size distribution with good dispersity and pH sensitivity	643:713	The obtained composite nanoparticles show a homogeneous size distribution with good dispersity and pH sensitivity.					
31082187	2	44	theme	clinical	267:274	arg1	application					276:286	its clinical application	263:286	its clinical application	263:286	However, its clinical application is severely limited because of insufficient tumor vascular penetration.					
31082187	1	45	theme	drug	157:160	arg1	carrier					162:168	a drug carrier	155:168	a drug carrier	155:168	Nanodiamond as a drug carrier is of great significance in improving cancer therapy by overcoming chemoresistance.					
31082187	0	46	theme	Pullulan-all-trans-Retinal	69:94	arg1	Nanoparticles					96:108	Pullulan-all-trans-Retinal Nanoparticles	69:108	Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy	69:137	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.					
31082187	8	47	theme	new	1115:1117	arg1	method					1154:1159	a powerful method	1143:1159	a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy	1143:1294	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	8	47	theme	new	1115:1117	arg1	strategy					1119:1126	This new strategy	1110:1126	This new strategy	1110:1126	This new strategy might serve as a powerful method to address the limitation of nanodiamonds and provide innovative ideas for the application of nanoparticles in clinical cancer therapy.					
31082187	6	48	theme	dosage	949:954	arg1	result					822:827	a result	820:827	a result	820:827	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	48	theme	dosage	949:954	arg1	%					931:931	17.3%	927:931	17.3% of the injected dosage	927:954	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	48	theme	dosage	949:954	arg1	retention					848:856	the intracellular retention	830:856	the intracellular retention of DOX	830:863	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	48	theme	dosage	949:954	arg1	dosage					949:954	the injected dosage	936:954	the injected dosage	936:954	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	6	49	from	DOX	895:897	arg1	tissue					912:917	the tumor tissue	902:917	the tumor tissue	902:917	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	7	50	theme	antitumor	961:969	arg1	effect					971:976	The antitumor effect	957:976	The antitumor effect of this combined strategy	957:1002	The antitumor effect of this combined strategy was remarkably improved in both the DOX-sensitive HepG2 and DOX-resistant HepG2/ADR tumor models in vivo.					
31082187	0	51	theme	Effective	114:122	arg1	Therapy					131:137	Effective Cancer Therapy	114:137	Effective Cancer Therapy	114:137	Ultrasound-Enhanced Delivery of Doxorubicin-Loaded Nanodiamonds from Pullulan-all-trans-Retinal Nanoparticles for Effective Cancer Therapy.					
31082187	6	52	theme	injected	940:947	arg1	dosage					949:954	the injected dosage	936:954	the injected dosage	936:954	As a result, the intracellular retention of DOX was efficiently enhanced, and DOX in the tumor tissue reached 17.3% of the injected dosage.					
31082187	1	53	theme	great	176:180	arg1	significance					182:193	great significance	176:193	great significance	176:193	Nanodiamond as a drug carrier is of great significance in improving cancer therapy by overcoming chemoresistance.					
31082187	7	54	dep	in	1101:1102	arg1	vivo					1104:1107	vivo	1104:1107	vivo	1104:1107	The antitumor effect of this combined strategy was remarkably improved in both the DOX-sensitive HepG2 and DOX-resistant HepG2/ADR tumor models in vivo.					
31082187	7	55	theme	DOX-sensitive	1040:1052	arg1	HepG2					1054:1058	the DOX-sensitive HepG2	1036:1058	the DOX-sensitive HepG2	1036:1058	The antitumor effect of this combined strategy was remarkably improved in both the DOX-sensitive HepG2 and DOX-resistant HepG2/ADR tumor models in vivo.					
29941663	6	0	theme	P-selectin	889:898	arg1	[CLA					962:965	i.e., cutaneous lymphocyte Ag [CLA	932:965	[CLA	962:965	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	0	theme	P-selectin	889:898	arg1	PSGL-1					923:928	PSGL-1	923:928	PSGL-1	923:928	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	0	theme	P-selectin	889:898	arg1	ligand-1					913:920	P-selectin glycoprotein ligand-1	889:920	P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA])	889:967	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	7	1	theme	PSGL-1	1156:1161	arg1	engagement					1163:1172	PSGL-1 engagement	1156:1172	PSGL-1 engagement	1156:1172	To elucidate the effect of CD44 or PSGL-1 engagement, mo-DCs were pretreated with their ligands.					
29941663	2	2	theme	receptor/ligand	352:366	arg1	interactions					368:379	receptor/ligand interactions	352:379	receptor/ligand interactions	352:379	Transendothelial migration (TEM) is dictated by an ordered cascade of receptor/ligand interactions.					
29941663	10	3	theme	administered	1671:1682	arg1	mo-DCs					1691:1696	systemically administered CD14-S mo-DCs	1658:1696	systemically administered CD14-S mo-DCs	1658:1696	These findings highlight structural and functional pleiotropism of CD44 in priming TEM of mo-DCs and suggest that strategies to enforce HCELL expression may boost TEM of systemically administered CD14-S mo-DCs.					
29941663	6	4	theme	E-selectin	856:865	arg1	glycoforms					875:884	E-selectin binding glycoforms	856:884	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL])	856:1040	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	2	5	theme	Transendothelial	282:297	arg1	TEM					310:312	TEM	310:312	TEM	310:312	Transendothelial migration (TEM) is dictated by an ordered cascade of receptor/ligand interactions.					
29941663	2	5	theme	Transendothelial	282:297	arg1	migration					299:307	Transendothelial migration	282:307	Transendothelial migration (TEM)	282:313	Transendothelial migration (TEM) is dictated by an ordered cascade of receptor/ligand interactions.					
29941663	1	6	theme	immunotherapeutics	174:191	arg1	success					137:143	The success	133:143	The success of dendritic cell (DC)-based immunotherapeutics	133:191	The success of dendritic cell (DC)-based immunotherapeutics critically hinges on the capacity of the vascularly administered cells to enter tissues.					
29941663	4	7	theme	PA-S	652:655	arg1	cells					657:661	PA-S cells	652:661	PA-S cells	652:661	Without chemokine input, CD14-S cells undergo greater TEM than PA-S cells over TNF-α-stimulated HUVECs.					
29941663	10	8	theme	structural	1513:1522	arg1	pleiotropism					1539:1550	structural and functional pleiotropism	1513:1550	structural and functional pleiotropism of CD44	1513:1558	These findings highlight structural and functional pleiotropism of CD44 in priming TEM of mo-DCs and suggest that strategies to enforce HCELL expression may boost TEM of systemically administered CD14-S mo-DCs.					
29941663	3	9	theme	adherence	561:569	arg1	PA-S					582:585	PA-S	582:585	PA-S	582:585	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	9	theme	adherence	561:569	arg1	selection					571:579	plastic adherence selection	553:579	plastic adherence selection (PA-S)	553:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	9	theme	adherence	561:569	arg1	effectors					427:435	the key molecular effectors	409:435	the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S)	409:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	5	10	theme	PA-S	843:846	arg1	mo-DCs					848:853	PA-S mo-DCs	843:853	PA-S mo-DCs	843:853	TEM of CD14-S mo-DCs is E-selectin/very late Ag-4 (VLA-4) dependent, and engagement of E-selectin ligands activates VLA-4 on CD14-S mo-DCs but not on PA-S mo-DCs.					
29941663	4	11	theme	CD14-S	614:619	arg1	cells					621:625	CD14-S cells	614:625	CD14-S cells	614:625	Without chemokine input, CD14-S cells undergo greater TEM than PA-S cells over TNF-α-stimulated HUVECs.					
29941663	10	12	dep	strategies	1602:1611	arg1	enforce					1616:1622	enforce	1616:1622	to enforce HCELL expression	1613:1639	These findings highlight structural and functional pleiotropism of CD44 in priming TEM of mo-DCs and suggest that strategies to enforce HCELL expression may boost TEM of systemically administered CD14-S mo-DCs.					
29941663	10	13	theme	mo-DCs	1691:1696	arg1	TEM					1651:1653	TEM	1651:1653	TEM of systemically administered CD14-S mo-DCs	1651:1696	These findings highlight structural and functional pleiotropism of CD44 in priming TEM of mo-DCs and suggest that strategies to enforce HCELL expression may boost TEM of systemically administered CD14-S mo-DCs.					
29941663	9	14	theme	cell	1373:1376	arg1	exofucosylation					1386:1400	cell surface exofucosylation	1373:1400	cell surface exofucosylation	1373:1400	HCELL expression on CD14-S mo-DC can be enforced by cell surface exofucosylation, yielding increased TEM in vitro and enhanced extravasation into bone marrow in vivo.					
29941663	5	15	theme	CD14-S	700:705	arg1	mo-DCs					707:712	CD14-S mo-DCs	700:712	CD14-S mo-DCs	700:712	TEM of CD14-S mo-DCs is E-selectin/very late Ag-4 (VLA-4) dependent, and engagement of E-selectin ligands activates VLA-4 on CD14-S mo-DCs but not on PA-S mo-DCs.					
29941663	5	16	theme	ligands	791:797	arg1	engagement					766:775	engagement	766:775	engagement of E-selectin ligands	766:797	TEM of CD14-S mo-DCs is E-selectin/very late Ag-4 (VLA-4) dependent, and engagement of E-selectin ligands activates VLA-4 on CD14-S mo-DCs but not on PA-S mo-DCs.					
29941663	8	17	dep	triggers	1252:1259	arg1	whereas					1287:1293	whereas	1287:1293	whereas	1287:1293	Ligation of CD44 on CD14-S mo-DCs triggers VLA-4 activation and TEM, whereas PSGL-1 ligation does not.					
29941663	10	18	theme	functional	1528:1537	arg1	pleiotropism					1539:1550	structural and functional pleiotropism	1513:1550	structural and functional pleiotropism of CD44	1513:1558	These findings highlight structural and functional pleiotropism of CD44 in priming TEM of mo-DCs and suggest that strategies to enforce HCELL expression may boost TEM of systemically administered CD14-S mo-DCs.					
29941663	5	19	from	VLA-4	809:813	arg1	mo-DCs					848:853	PA-S mo-DCs	843:853	PA-S mo-DCs	843:853	TEM of CD14-S mo-DCs is E-selectin/very late Ag-4 (VLA-4) dependent, and engagement of E-selectin ligands activates VLA-4 on CD14-S mo-DCs but not on PA-S mo-DCs.					
29941663	5	19	from	VLA-4	809:813	arg1	mo-DCs					825:830	CD14-S mo-DCs	818:830	CD14-S mo-DCs	818:830	TEM of CD14-S mo-DCs is E-selectin/very late Ag-4 (VLA-4) dependent, and engagement of E-selectin ligands activates VLA-4 on CD14-S mo-DCs but not on PA-S mo-DCs.					
29941663	6	20	theme	ligand-1	913:920	arg1	glycoforms					875:884	E-selectin binding glycoforms	856:884	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL])	856:1040	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	1	21	theme	dendritic	148:156	arg1	DC					164:165	DC	164:165	DC	164:165	The success of dendritic cell (DC)-based immunotherapeutics critically hinges on the capacity of the vascularly administered cells to enter tissues.					
29941663	1	21	theme	dendritic	148:156	arg1	cell					158:161	dendritic cell	148:161	dendritic cell (DC)-based immunotherapeutics	148:191	The success of dendritic cell (DC)-based immunotherapeutics critically hinges on the capacity of the vascularly administered cells to enter tissues.					
29941663	6	22	theme	CD44	973:976	arg1	glycoforms					875:884	E-selectin binding glycoforms	856:884	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL])	856:1040	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	0	23	theme	Human	57:61	arg1	Cells					90:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.					
29941663	6	24	gly	glycoprotein	900:911	arg1	glycoprotein					900:911	P-selectin glycoprotein ligand-1	889:920	P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA])	889:967	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	0	25	theme	Dendritic	80:88	arg1	Cells					90:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.					
29941663	9	26	theme	enhanced	1439:1446	arg1	extravasation					1448:1460	enhanced extravasation	1439:1460	enhanced extravasation into bone marrow in vivo	1439:1485	HCELL expression on CD14-S mo-DC can be enforced by cell surface exofucosylation, yielding increased TEM in vitro and enhanced extravasation into bone marrow in vivo.					
29941663	0	27	from	Ligation	0:7	arg1	Cells					90:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.					
29941663	8	28	theme	VLA-4	1261:1265	arg1	activation					1267:1276	VLA-4 activation	1261:1276	VLA-4 activation	1261:1276	Ligation of CD44 on CD14-S mo-DCs triggers VLA-4 activation and TEM, whereas PSGL-1 ligation does not.					
29941663	6	29	theme	lymphocyte	948:957	arg1	[CLA					962:965	i.e., cutaneous lymphocyte Ag [CLA	932:965	[CLA	962:965	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	29	theme	lymphocyte	948:957	arg1	ligand-1					913:920	P-selectin glycoprotein ligand-1	889:920	P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA])	889:967	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	3	30	theme	TEM	440:442	arg1	effectors					427:435	the key molecular effectors	409:435	the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S)	409:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	30	theme	TEM	440:442	arg1	selection					571:579	plastic adherence selection	553:579	plastic adherence selection (PA-S)	553:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	30	theme	TEM	440:442	arg1	selection					530:538	CD14 selection	525:538	CD14 selection (CD14-S)	525:547	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	6	31	theme	[HCELL	1033:1038	arg1	E-selectin/L-selectin					1004:1024	hematopoietic cell E-selectin/L-selectin	985:1024	hematopoietic cell E-selectin/L-selectin ligand [HCELL	985:1038	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	32	dep	CD44	973:976	arg1	E-selectin/L-selectin					1004:1024	hematopoietic cell E-selectin/L-selectin	985:1024	hematopoietic cell E-selectin/L-selectin ligand [HCELL	985:1038	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	0	33	theme	Monocyte-Derived	63:78	arg1	Cells					90:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.					
29941663	5	34	theme	E-selectin	780:789	arg1	ligands					791:797	E-selectin ligands	780:797	E-selectin ligands	780:797	TEM of CD14-S mo-DCs is E-selectin/very late Ag-4 (VLA-4) dependent, and engagement of E-selectin ligands activates VLA-4 on CD14-S mo-DCs but not on PA-S mo-DCs.					
29941663	8	35	theme	PSGL-1	1295:1300	arg1	ligation					1302:1309	PSGL-1 ligation	1295:1309	PSGL-1 ligation	1295:1309	Ligation of CD44 on CD14-S mo-DCs triggers VLA-4 activation and TEM, whereas PSGL-1 ligation does not.					
29941663	3	36	theme	DCs	470:472	arg1	TEM					440:442	TEM	440:442	TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods	440:522	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	6	37	theme	hematopoietic	985:997	arg1	E-selectin/L-selectin					1004:1024	hematopoietic cell E-selectin/L-selectin	985:1024	hematopoietic cell E-selectin/L-selectin ligand [HCELL	985:1038	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	3	38	theme	key	413:415	arg1	effectors					427:435	the key molecular effectors	409:435	the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S)	409:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	38	theme	key	413:415	arg1	selection					571:579	plastic adherence selection	553:579	plastic adherence selection (PA-S)	553:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	38	theme	key	413:415	arg1	selection					530:538	CD14 selection	525:538	CD14 selection (CD14-S)	525:547	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	39	theme	human	447:451	arg1	mo-DCs					475:480	mo-DCs	475:480	mo-DCs	475:480	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	39	theme	human	447:451	arg1	DCs					470:472	human monocyte-derived DCs	447:472	human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods	447:522	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	0	40	theme	Glycoform	21:29	arg1	HCELL					31:35	the CD44 Glycoform HCELL	12:35	the CD44 Glycoform HCELL	12:35	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.					
29941663	9	41	from	expression	1327:1336	arg1	mo-DC					1348:1352	mo-DC	1348:1352	mo-DC	1348:1352	HCELL expression on CD14-S mo-DC can be enforced by cell surface exofucosylation, yielding increased TEM in vitro and enhanced extravasation into bone marrow in vivo.					
29941663	6	42	dep	expressed	1051:1059	arg1	both					1046:1049	both	1046:1049	both	1046:1049	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	10	43	theme	HCELL	1624:1628	arg1	expression					1630:1639	HCELL expression	1624:1639	HCELL expression	1624:1639	These findings highlight structural and functional pleiotropism of CD44 in priming TEM of mo-DCs and suggest that strategies to enforce HCELL expression may boost TEM of systemically administered CD14-S mo-DCs.					
29941663	2	44	theme	ordered	333:339	arg1	cascade					341:347	an ordered cascade	330:347	an ordered cascade of receptor/ligand interactions	330:379	Transendothelial migration (TEM) is dictated by an ordered cascade of receptor/ligand interactions.					
29941663	10	45	theme	mo-DCs	1578:1583	arg1	TEM					1571:1573	TEM	1571:1573	TEM of mo-DCs	1571:1583	These findings highlight structural and functional pleiotropism of CD44 in priming TEM of mo-DCs and suggest that strategies to enforce HCELL expression may boost TEM of systemically administered CD14-S mo-DCs.					
29941663	1	46	theme	administered	245:256	arg1	cells					258:262	the vascularly administered cells	230:262	the vascularly administered cells	230:262	The success of dendritic cell (DC)-based immunotherapeutics critically hinges on the capacity of the vascularly administered cells to enter tissues.					
29941663	6	47	theme	glycoprotein	900:911	arg1	[CLA					962:965	i.e., cutaneous lymphocyte Ag [CLA	932:965	[CLA	962:965	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	47	theme	glycoprotein	900:911	arg1	PSGL-1					923:928	PSGL-1	923:928	PSGL-1	923:928	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	47	theme	glycoprotein	900:911	arg1	ligand-1					913:920	P-selectin glycoprotein ligand-1	889:920	P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA])	889:967	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	4	48	theme	chemokine	597:605	arg1	input					607:611	chemokine input	597:611	chemokine input	597:611	Without chemokine input, CD14-S cells undergo greater TEM than PA-S cells over TNF-α-stimulated HUVECs.					
29941663	2	49	theme	interactions	368:379	arg1	cascade					341:347	an ordered cascade	330:347	an ordered cascade of receptor/ligand interactions	330:379	Transendothelial migration (TEM) is dictated by an ordered cascade of receptor/ligand interactions.					
29941663	9	50	theme	HCELL	1321:1325	arg1	expression					1327:1336	HCELL expression	1321:1336	HCELL expression on CD14-S mo-DC	1321:1352	HCELL expression on CD14-S mo-DC can be enforced by cell surface exofucosylation, yielding increased TEM in vitro and enhanced extravasation into bone marrow in vivo.					
29941663	3	51	link	monocyte-derived	453:468	arg1	mo-DCs					475:480	mo-DCs	475:480	mo-DCs	475:480	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	51	link	monocyte-derived	453:468	arg1	DCs					470:472	human monocyte-derived DCs	447:472	human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods	447:522	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	7	52	theme	engagement	1163:1172	arg1	effect					1138:1143	the effect	1134:1143	the effect of CD44 or PSGL-1 engagement	1134:1172	To elucidate the effect of CD44 or PSGL-1 engagement, mo-DCs were pretreated with their ligands.					
29941663	10	53	theme	CD14-S	1684:1689	arg1	mo-DCs					1691:1696	systemically administered CD14-S mo-DCs	1658:1696	systemically administered CD14-S mo-DCs	1658:1696	These findings highlight structural and functional pleiotropism of CD44 in priming TEM of mo-DCs and suggest that strategies to enforce HCELL expression may boost TEM of systemically administered CD14-S mo-DCs.					
29941663	6	54	theme	binding	867:873	arg1	glycoforms					875:884	E-selectin binding glycoforms	856:884	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL])	856:1040	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	55	theme	PA-S	1108:1111	arg1	mo-DCs					1113:1118	PA-S mo-DCs	1108:1118	PA-S mo-DCs	1108:1118	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	3	56	theme	CD14	525:528	arg1	effectors					427:435	the key molecular effectors	409:435	the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S)	409:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	56	theme	CD14	525:528	arg1	CD14-S					541:546	CD14-S	541:546	CD14-S	541:546	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	56	theme	CD14	525:528	arg1	selection					530:538	CD14 selection	525:538	CD14 selection (CD14-S)	525:547	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	1	57	theme	-based	167:172	arg1	immunotherapeutics					174:191	dendritic cell (DC)-based immunotherapeutics	148:191	dendritic cell (DC)-based immunotherapeutics	148:191	The success of dendritic cell (DC)-based immunotherapeutics critically hinges on the capacity of the vascularly administered cells to enter tissues.					
29941663	4	58	theme	greater	635:641	arg1	TEM					643:645	greater TEM	635:645	greater TEM than PA-S cells	635:661	Without chemokine input, CD14-S cells undergo greater TEM than PA-S cells over TNF-α-stimulated HUVECs.					
29941663	3	59	theme	plastic	553:559	arg1	PA-S					582:585	PA-S	582:585	PA-S	582:585	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	59	theme	plastic	553:559	arg1	selection					571:579	plastic adherence selection	553:579	plastic adherence selection (PA-S)	553:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	59	theme	plastic	553:559	arg1	effectors					427:435	the key molecular effectors	409:435	the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S)	409:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	6	60	theme	cutaneous	938:946	arg1	[CLA					962:965	i.e., cutaneous lymphocyte Ag [CLA	932:965	[CLA	962:965	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	60	theme	cutaneous	938:946	arg1	ligand-1					913:920	P-selectin glycoprotein ligand-1	889:920	P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA])	889:967	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	61	theme	i.e.	932:935	arg1	[CLA					962:965	i.e., cutaneous lymphocyte Ag [CLA	932:965	[CLA	962:965	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	61	theme	i.e.	932:935	arg1	ligand-1					913:920	P-selectin glycoprotein ligand-1	889:920	P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA])	889:967	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	4	62	theme	TNF-α-stimulated	668:683	arg1	HUVECs					685:690	TNF-α-stimulated HUVECs	668:690	TNF-α-stimulated HUVECs	668:690	Without chemokine input, CD14-S cells undergo greater TEM than PA-S cells over TNF-α-stimulated HUVECs.					
29941663	10	63	theme	CD44	1555:1558	arg1	pleiotropism					1539:1550	structural and functional pleiotropism	1513:1550	structural and functional pleiotropism of CD44	1513:1558	These findings highlight structural and functional pleiotropism of CD44 in priming TEM of mo-DCs and suggest that strategies to enforce HCELL expression may boost TEM of systemically administered CD14-S mo-DCs.					
29941663	9	64	theme	surface	1378:1384	arg1	exofucosylation					1386:1400	cell surface exofucosylation	1373:1400	cell surface exofucosylation	1373:1400	HCELL expression on CD14-S mo-DC can be enforced by cell surface exofucosylation, yielding increased TEM in vitro and enhanced extravasation into bone marrow in vivo.					
29941663	5	65	theme	mo-DCs	707:712	arg1	TEM					693:695	TEM	693:695	TEM of CD14-S mo-DCs	693:712	TEM of CD14-S mo-DCs is E-selectin/very late Ag-4 (VLA-4) dependent, and engagement of E-selectin ligands activates VLA-4 on CD14-S mo-DCs but not on PA-S mo-DCs.					
29941663	9	66	theme	increased	1412:1420	arg1	TEM					1422:1424	increased TEM	1412:1424	increased TEM in vitro	1412:1433	HCELL expression on CD14-S mo-DC can be enforced by cell surface exofucosylation, yielding increased TEM in vitro and enhanced extravasation into bone marrow in vivo.					
29941663	7	67	theme	CD44	1148:1151	arg1	effect					1138:1143	the effect	1134:1143	the effect of CD44 or PSGL-1 engagement	1134:1172	To elucidate the effect of CD44 or PSGL-1 engagement, mo-DCs were pretreated with their ligands.					
29941663	1	68	theme	cell	158:161	arg1	immunotherapeutics					174:191	dendritic cell (DC)-based immunotherapeutics	148:191	dendritic cell (DC)-based immunotherapeutics	148:191	The success of dendritic cell (DC)-based immunotherapeutics critically hinges on the capacity of the vascularly administered cells to enter tissues.					
29941663	8	69	theme	CD44	1230:1233	arg1	Ligation					1218:1225	Ligation	1218:1225	Ligation of CD44 on CD14-S mo-DCs	1218:1250	Ligation of CD44 on CD14-S mo-DCs triggers VLA-4 activation and TEM, whereas PSGL-1 ligation does not.					
29941663	8	70	theme	CD14-S	1238:1243	arg1	mo-DCs					1245:1250	CD14-S mo-DCs	1238:1250	CD14-S mo-DCs	1238:1250	Ligation of CD44 on CD14-S mo-DCs triggers VLA-4 activation and TEM, whereas PSGL-1 ligation does not.					
29941663	6	71	gly	glycoforms	875:884	arg1	[CLA					962:965	i.e., cutaneous lymphocyte Ag [CLA	932:965	[CLA	962:965	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	71	gly	glycoforms	875:884	arg1	E-selectin					856:865	E-selectin binding glycoforms	856:884	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL])	856:1040	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	71	gly	glycoforms	875:884	arg1	ligand-1					913:920	P-selectin glycoprotein ligand-1	889:920	P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA])	889:967	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	71	gly	glycoforms	875:884	arg1	binding					867:873	E-selectin binding glycoforms	856:884	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL])	856:1040	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	71	gly	glycoforms	875:884	arg1	CD44					973:976	CD44	973:976	CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL])	973:1040	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	71	gly	glycoforms	875:884	arg1	PSGL-1					923:928	PSGL-1	923:928	PSGL-1	923:928	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	0	72	theme	Transendothelial	105:120	arg1	Migration					122:130	Transendothelial Migration	105:130	Transendothelial Migration	105:130	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.					
29941663	8	73	from	Ligation	1218:1225	arg1	mo-DCs					1245:1250	CD14-S mo-DCs	1238:1250	CD14-S mo-DCs	1238:1250	Ligation of CD44 on CD14-S mo-DCs triggers VLA-4 activation and TEM, whereas PSGL-1 ligation does not.					
29941663	6	74	theme	Ag	959:960	arg1	[CLA					962:965	i.e., cutaneous lymphocyte Ag [CLA	932:965	[CLA	962:965	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	6	74	theme	Ag	959:960	arg1	ligand-1					913:920	P-selectin glycoprotein ligand-1	889:920	P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA])	889:967	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	9	75	theme	bone	1467:1470	arg1	marrow					1472:1477	bone marrow	1467:1477	bone marrow in vivo	1467:1485	HCELL expression on CD14-S mo-DC can be enforced by cell surface exofucosylation, yielding increased TEM in vitro and enhanced extravasation into bone marrow in vivo.					
29941663	6	76	dep	E-selectin/L-selectin	1004:1024	arg1	i.e.					979:982	i.e.	979:982	i.e.	979:982	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	0	77	theme	CD44	16:19	arg1	HCELL					31:35	the CD44 Glycoform HCELL	12:35	the CD44 Glycoform HCELL	12:35	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.					
29941663	6	78	theme	cell	999:1002	arg1	E-selectin/L-selectin					1004:1024	hematopoietic cell E-selectin/L-selectin	985:1024	hematopoietic cell E-selectin/L-selectin ligand [HCELL	985:1038	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	0	79	theme	HCELL	31:35	arg1	Ligation					0:7	Ligation	0:7	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells	0:94	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.					
29941663	5	80	theme	CD14-S	818:823	arg1	mo-DCs					825:830	CD14-S mo-DCs	818:830	CD14-S mo-DCs	818:830	TEM of CD14-S mo-DCs is E-selectin/very late Ag-4 (VLA-4) dependent, and engagement of E-selectin ligands activates VLA-4 on CD14-S mo-DCs but not on PA-S mo-DCs.					
29941663	3	81	dep	effectors	427:435	arg1	PA-S					582:585	PA-S	582:585	PA-S	582:585	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	81	dep	effectors	427:435	arg1	effectors					427:435	the key molecular effectors	409:435	the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S)	409:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	81	dep	effectors	427:435	arg1	selection					571:579	plastic adherence selection	553:579	plastic adherence selection (PA-S)	553:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	81	dep	effectors	427:435	arg1	CD14-S					541:546	CD14-S	541:546	CD14-S	541:546	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	81	dep	effectors	427:435	arg1	selection					530:538	CD14 selection	525:538	CD14 selection (CD14-S)	525:547	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	82	theme	molecular	417:425	arg1	effectors					427:435	the key molecular effectors	409:435	the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S)	409:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	82	theme	molecular	417:425	arg1	selection					571:579	plastic adherence selection	553:579	plastic adherence selection (PA-S)	553:586	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	82	theme	molecular	417:425	arg1	selection					530:538	CD14 selection	525:538	CD14 selection (CD14-S)	525:547	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	83	theme	monocyte-derived	453:468	arg1	mo-DCs					475:480	mo-DCs	475:480	mo-DCs	475:480	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	3	83	theme	monocyte-derived	453:468	arg1	DCs					470:472	human monocyte-derived DCs	447:472	human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods	447:522	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	0	84	theme	Culture-Expanded	40:55	arg1	Cells					90:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Culture-Expanded Human Monocyte-Derived Dendritic Cells	40:94	Ligation of the CD44 Glycoform HCELL on Culture-Expanded Human Monocyte-Derived Dendritic Cells Programs Transendothelial Migration.					
29941663	3	85	theme	relevant	507:514	arg1	methods					516:522	clinically relevant methods	496:522	clinically relevant methods	496:522	In this study, we examined the key molecular effectors of TEM of human monocyte-derived DCs (mo-DCs) generated by clinically relevant methods: CD14 selection (CD14-S) and plastic adherence selection (PA-S).					
29941663	6	86	theme	CD14-S	1064:1069	arg1	mo-DCs					1071:1076	CD14-S mo-DCs	1064:1076	CD14-S mo-DCs	1064:1076	E-selectin binding glycoforms of P-selectin glycoprotein ligand-1 (PSGL-1) (i.e., cutaneous lymphocyte Ag [CLA]) and CD44 (i.e., hematopoietic cell E-selectin/L-selectin ligand [HCELL]) are both expressed on CD14-S mo-DCs, but only CLA is expressed on PA-S mo-DCs.					
29941663	1	87	theme	cells	258:262	arg1	capacity					218:225	the capacity	214:225	the capacity of the vascularly administered cells to enter tissues	214:279	The success of dendritic cell (DC)-based immunotherapeutics critically hinges on the capacity of the vascularly administered cells to enter tissues.					
31857120	7	0	theme	clinical	1668:1675	arg1	research					1677:1684	clinical research	1668:1684	clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors	1668:1786	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	1	1	from	brain	282:286	arg1	shedding					268:275	ganglioside (GG) shedding	251:275	ganglioside (GG) shedding from brain to CSF	251:293	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	1	from	brain	282:286	arg1	availability					192:203	better availability	185:203	better availability for research than tissue biopsies	185:237	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	1	from	brain	282:286	arg1	renewal					173:179	its permanent renewal	159:179	its permanent renewal	159:179	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	1	from	brain	282:286	arg1	proximity					104:112	The proximity	100:112	The proximity of cerebrospinal fluid (CSF) with the brain	100:156	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	2	2	theme	classes	569:575	arg1	measurements					537:548	concentration measurements	523:548	concentration measurements of the predominant classes	523:575	Currently, the investigation of CSF gangliosides is focused on concentration measurements of the predominant classes and much less on their profiling and structural analysis.					
31857120	3	3	theme	robust	959:964	arg1	database					980:987	the first robust mass spectral database	949:987	the first robust mass spectral database of CSF gangliosides	949:1007	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	4	theme	mobility	699:706	arg1	spectrometry					724:735	ion mobility separation mass spectrometry	695:735	ion mobility separation mass spectrometry (IMS MS)	695:744	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	4	theme	mobility	699:706	arg1	MS					742:743	IMS MS	738:743	IMS MS	738:743	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	1	5	theme	central	408:414	arg1	system					424:429	central nervous system	408:429	central nervous system biomarkers expressed in CSF	408:457	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	3	6	theme	first	862:866	arg1	MS					877:878	the first time IMS MS	858:878	the first time IMS MS for the exploration of human CSF gangliosidome	858:925	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	1	7	theme	system	424:429	arg1	biomarkers					431:440	central nervous system biomarkers	408:440	central nervous system biomarkers expressed in CSF	408:457	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	8	theme	permanent	163:171	arg1	renewal					173:179	its permanent renewal	159:179	its permanent renewal	159:179	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	3	9	theme	spectral	971:978	arg1	database					980:987	the first robust mass spectral database	949:987	the first robust mass spectral database of CSF gangliosides	949:1007	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	10	theme	mass	719:722	arg1	spectrometry					724:735	ion mobility separation mass spectrometry	695:735	ion mobility separation mass spectrometry (IMS MS)	695:744	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	10	theme	mass	719:722	arg1	MS					742:743	IMS MS	738:743	IMS MS	738:743	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	1	11	theme	protocols	331:339	arg1	development					316:326	the development	312:326	the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF	312:457	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	6	12	theme	GD2	1399:1401	arg1	structure					1366:1374	the structure	1362:1374	the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0)	1362:1413	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	6	12	theme	GD2	1399:1401	arg1	glycoforms					1420:1429	two glycoforms	1416:1429	two glycoforms exhibiting short carbohydrate chains found in CSF	1416:1479	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	4	13	theme	distinct	1055:1062	arg1	species					1067:1073	113 distinct GG species	1051:1073	113 distinct GG species in CSF	1051:1080	IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain.					
31857120	3	14	theme	present	820:826	arg1	study					828:832	the present study	816:832	the present study	816:832	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	6	15	located	found	1468:1472	arg2	chains					1461:1466	short carbohydrate chains	1442:1466	short carbohydrate chains found in CSF	1442:1479	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	6	15	located	found	1468:1472	arg1	CSF					1477:1479	CSF	1477:1479	CSF	1477:1479	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	1	16	theme	cerebrospinal	117:129	arg1	fluid					131:135	cerebrospinal fluid	117:135	cerebrospinal fluid (CSF) with the brain	117:156	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	16	theme	cerebrospinal	117:129	arg1	CSF					138:140	CSF	138:140	CSF	138:140	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	7	17	theme	similar	1605:1611	arg1	pattern					1625:1631	a similar ganglioside pattern	1603:1631	a similar ganglioside pattern	1603:1631	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	7	17	theme	similar	1605:1611	arg1	finding					1636:1642	a finding	1634:1642	a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors	1634:1786	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	7	17	theme	similar	1605:1611	arg1	useful					1658:1663	useful	1658:1663	useful	1658:1663	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	1	18	theme	glycoconjugates	375:389	arg1	discovery					395:403	discovery	395:403	discovery of central nervous system biomarkers expressed in CSF	395:457	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	18	theme	glycoconjugates	375:389	arg1	characterization					349:364	the characterization	345:364	the characterization of these glycoconjugates	345:389	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	5	19	theme	several	1214:1220	arg1	components					1222:1231	CSF several components	1210:1231	CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains	1210:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	6	20	theme	short	1442:1446	arg1	chains					1461:1466	short carbohydrate chains	1442:1466	short carbohydrate chains found in CSF	1442:1479	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	4	21	located	detected	1126:1133	arg2	species					1118:1124	the species	1114:1124	the species detected in human brain	1114:1148	IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain.					
31857120	4	21	located	detected	1126:1133	arg1	brain					1144:1148	human brain	1138:1148	human brain	1138:1148	IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain.					
31857120	3	22	theme	GGs	808:810	arg1	determination					779:791	compositional and structural determination	750:791	compositional and structural determination of human brain GGs	750:810	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	0	23	theme	mobility	72:79	arg1	spectrometry					86:97	ion mobility mass spectrometry	68:97	ion mobility mass spectrometry	68:97	Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry.					
31857120	7	24	theme	human	1578:1582	arg1	CSF					1584:1586	human CSF	1578:1586	human CSF	1578:1586	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	2	25	theme	CSF	492:494	arg1	gangliosides					496:507	CSF gangliosides	492:507	CSF gangliosides	492:507	Currently, the investigation of CSF gangliosides is focused on concentration measurements of the predominant classes and much less on their profiling and structural analysis.					
31857120	3	26	theme	human	796:800	arg1	GGs					808:810	human brain GGs	796:810	human brain GGs	796:810	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	27	theme	gangliosides	996:1007	arg1	database					980:987	the first robust mass spectral database	949:987	the first robust mass spectral database of CSF gangliosides	949:1007	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	28	theme	time	868:871	arg1	MS					877:878	the first time IMS MS	858:878	the first time IMS MS for the exploration of human CSF gangliosidome	858:925	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	5	29	theme	odd	1261:1263	arg1	number					1265:1270	odd number	1261:1270	odd number of carbon atoms and/or short glycan chains	1261:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	4	30	theme	human	1138:1142	arg1	brain					1144:1148	human brain	1138:1148	human brain	1138:1148	IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain.					
31857120	0	31	theme	Cerebrospinal	0:12	arg1	fluid					14:18	Cerebrospinal fluid	0:18	Cerebrospinal fluid	0:18	Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry.					
31857120	3	32	theme	CSF	909:911	arg1	gangliosidome					913:925	human CSF gangliosidome	903:925	human CSF gangliosidome	903:925	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	5	33	theme	chains	1308:1313	arg1	number					1265:1270	odd number	1261:1270	odd number of carbon atoms and/or short glycan chains	1261:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	6	34	theme	tandem	1319:1324	arg1	MS/MS					1330:1334	MS/MS	1330:1334	MS/MS	1330:1334	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	6	34	theme	tandem	1319:1324	arg1	MS					1326:1327	tandem MS	1319:1327	tandem MS (MS/MS)	1319:1335	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	7	35	theme	neurodegenerative	1744:1760	arg1	diseases					1762:1769	neurodegenerative diseases	1744:1769	neurodegenerative diseases	1744:1769	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	5	36	theme	atoms	1282:1286	arg1	number					1265:1270	odd number	1261:1270	odd number of carbon atoms and/or short glycan chains	1261:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	6	37	theme	GD3	1379:1381	arg1	structure					1366:1374	the structure	1362:1374	the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0)	1362:1413	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	6	37	theme	GD3	1379:1381	arg1	glycoforms					1420:1429	two glycoforms	1416:1429	two glycoforms exhibiting short carbohydrate chains found in CSF	1416:1479	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	5	38	theme	short	1295:1299	arg1	chains					1308:1313	short glycan chains	1295:1313	short glycan chains	1295:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	3	39	theme	first	953:957	arg1	database					980:987	the first robust mass spectral database	949:987	the first robust mass spectral database of CSF gangliosides	949:1007	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	7	40	from	useful	1658:1663	arg1	research					1677:1684	clinical research	1668:1684	clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors	1668:1786	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	4	41	theme	IMS	1010:1012	arg1	separation					1017:1026	IMS MS separation	1010:1026	IMS MS separation	1010:1026	IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain.					
31857120	2	42	theme	predominant	557:567	arg1	classes					569:575	the predominant classes	553:575	the predominant classes	553:575	Currently, the investigation of CSF gangliosides is focused on concentration measurements of the predominant classes and much less on their profiling and structural analysis.					
31857120	3	43	theme	separation	708:717	arg1	spectrometry					724:735	ion mobility separation mass spectrometry	695:735	ion mobility separation mass spectrometry (IMS MS)	695:744	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	43	theme	separation	708:717	arg1	MS					742:743	IMS MS	738:743	IMS MS	738:743	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	1	44	theme	nervous	416:422	arg1	system					424:429	central nervous system	408:429	central nervous system biomarkers expressed in CSF	408:457	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	3	45	theme	ion	695:697	arg1	spectrometry					724:735	ion mobility separation mass spectrometry	695:735	ion mobility separation mass spectrometry (IMS MS)	695:744	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	45	theme	ion	695:697	arg1	MS					742:743	IMS MS	738:743	IMS MS	738:743	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	1	46	theme	biomarkers	431:440	arg1	discovery					395:403	discovery	395:403	discovery of central nervous system biomarkers expressed in CSF	395:457	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	46	theme	biomarkers	431:440	arg1	characterization					349:364	the characterization	345:364	the characterization of these glycoconjugates	345:389	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	3	47	theme	mass	966:969	arg1	database					980:987	the first robust mass spectral database	949:987	the first robust mass spectral database of CSF gangliosides	949:1007	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	48	theme	IMS	738:740	arg1	spectrometry					724:735	ion mobility separation mass spectrometry	695:735	ion mobility separation mass spectrometry (IMS MS)	695:744	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	48	theme	IMS	738:740	arg1	MS					742:743	IMS MS	738:743	IMS MS	738:743	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	5	49	contain	containing	1233:1242	arg1	components					1222:1231	CSF several components	1210:1231	CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains	1210:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	5	49	contain	containing	1233:1242	arg2	acids					1250:1254	fatty acids	1244:1254	fatty acids with odd number of carbon atoms and/or short glycan chains	1244:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	4	50	theme	GG	1064:1065	arg1	species					1067:1073	113 distinct GG species	1051:1073	113 distinct GG species in CSF	1051:1080	IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain.					
31857120	3	51	theme	spectrometry	724:735	arg1	performance					680:690	recently the high performance	662:690	recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs	662:810	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	5	52	with	acids	1250:1254	arg1	number					1265:1270	odd number	1261:1270	odd number of carbon atoms and/or short glycan chains	1261:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	4	53	from	species	1067:1073	arg1	CSF					1078:1080	CSF	1078:1080	CSF	1078:1080	IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain.					
31857120	1	54	theme	better	185:190	arg1	availability					192:203	better availability	185:203	better availability for research than tissue biopsies	185:237	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	2	55	theme	gangliosides	496:507	arg1	investigation					475:487	the investigation	471:487	the investigation of CSF gangliosides	471:507	Currently, the investigation of CSF gangliosides is focused on concentration measurements of the predominant classes and much less on their profiling and structural analysis.					
31857120	3	56	theme	compositional	750:762	arg1	determination					779:791	compositional and structural determination	750:791	compositional and structural determination of human brain GGs	750:810	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	1	57	theme	fluid	131:135	arg1	shedding					268:275	ganglioside (GG) shedding	251:275	ganglioside (GG) shedding from brain to CSF	251:293	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	57	theme	fluid	131:135	arg1	availability					192:203	better availability	185:203	better availability for research than tissue biopsies	185:237	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	57	theme	fluid	131:135	arg1	renewal					173:179	its permanent renewal	159:179	its permanent renewal	159:179	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	1	57	theme	fluid	131:135	arg1	proximity					104:112	The proximity	100:112	The proximity of cerebrospinal fluid (CSF) with the brain	100:156	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	7	58	theme	ganglioside	1613:1623	arg1	pattern					1625:1631	a similar ganglioside pattern	1603:1631	a similar ganglioside pattern	1603:1631	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	7	58	theme	ganglioside	1613:1623	arg1	finding					1636:1642	a finding	1634:1642	a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors	1634:1786	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	7	58	theme	ganglioside	1613:1623	arg1	useful					1658:1663	useful	1658:1663	useful	1658:1663	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	2	59	theme	concentration	523:535	arg1	measurements					537:548	concentration measurements	523:548	concentration measurements of the predominant classes	523:575	Currently, the investigation of CSF gangliosides is focused on concentration measurements of the predominant classes and much less on their profiling and structural analysis.					
31857120	4	60	theme	similar	1090:1096	arg1	compositions					1098:1109	similar compositions	1090:1109	similar compositions	1090:1109	IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain.					
31857120	5	61	theme	CSF	1210:1212	arg1	components					1222:1231	CSF several components	1210:1231	CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains	1210:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	0	62	theme	ion	68:70	arg1	spectrometry					86:97	ion mobility mass spectrometry	68:97	ion mobility mass spectrometry	68:97	Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry.					
31857120	6	63	theme	carbohydrate	1448:1459	arg1	chains					1461:1466	short carbohydrate chains	1442:1466	short carbohydrate chains found in CSF	1442:1479	By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well.					
31857120	3	64	theme	structural	768:777	arg1	determination					779:791	compositional and structural determination	750:791	compositional and structural determination of human brain GGs	750:810	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	0	65	theme	mass	81:84	arg1	spectrometry					86:97	ion mobility mass spectrometry	68:97	ion mobility mass spectrometry	68:97	Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry.					
31857120	3	66	theme	CSF	992:994	arg1	gangliosides					996:1007	CSF gangliosides	992:1007	CSF gangliosides	992:1007	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	2	67	theme	structural	614:623	arg1	analysis					625:632	structural analysis	614:632	structural analysis	614:632	Currently, the investigation of CSF gangliosides is focused on concentration measurements of the predominant classes and much less on their profiling and structural analysis.					
31857120	3	68	theme	brain	802:806	arg1	GGs					808:810	human brain GGs	796:810	human brain GGs	796:810	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	1	69	with	fluid	131:135	arg1	brain					152:156	the brain	148:156	the brain	148:156	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	3	70	theme	IMS	873:875	arg1	MS					877:878	the first time IMS MS	858:878	the first time IMS MS for the exploration of human CSF gangliosidome	858:925	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	3	71	theme	gangliosidome	913:925	arg1	exploration					888:898	the exploration	884:898	the exploration of human CSF gangliosidome	884:925	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	7	72	theme	present	1561:1567	arg1	results					1569:1575	the present results	1557:1575	the present results	1557:1575	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	7	73	from	research	1677:1684	arg1	pattern					1625:1631	a similar ganglioside pattern	1603:1631	a similar ganglioside pattern	1603:1631	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	7	73	from	research	1677:1684	arg1	finding					1636:1642	a finding	1634:1642	a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors	1634:1786	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	7	73	from	research	1677:1684	arg1	useful					1658:1663	useful	1658:1663	useful	1658:1663	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	1	74	theme	ganglioside	251:261	arg1	shedding					268:275	ganglioside (GG) shedding	251:275	ganglioside (GG) shedding from brain to CSF	251:293	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	7	75	theme	brain	1775:1779	arg1	tumors					1781:1786	brain tumors	1775:1786	brain tumors	1775:1786	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	5	76	theme	fatty	1244:1248	arg1	acids					1250:1254	fatty acids	1244:1254	fatty acids with odd number of carbon atoms and/or short glycan chains	1244:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	1	77	theme	GG	264:265	arg1	shedding					268:275	ganglioside (GG) shedding	251:275	ganglioside (GG) shedding from brain to CSF	251:293	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	5	78	theme	glycan	1301:1306	arg1	chains					1308:1313	short glycan chains	1295:1313	short glycan chains	1295:1313	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	0	79	theme	gangliosides	52:63	arg1	fragmentation					35:47	fragmentation	35:47	fragmentation	35:47	Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry.					
31857120	0	79	theme	gangliosides	52:63	arg1	Profiling					21:29	Profiling	21:29	Profiling	21:29	Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry.					
31857120	7	80	theme	ganglioside	1710:1720	arg1	species					1722:1728	ganglioside species	1710:1728	ganglioside species associated to neurodegenerative diseases and brain tumors	1710:1786	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	7	81	theme	species	1722:1728	arg1	discovery					1697:1705	discovery	1697:1705	discovery of ganglioside species associated to neurodegenerative diseases and brain tumors	1697:1786	According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.					
31857120	0	82	dep	Profiling	21:29	arg1	fluid					14:18	Cerebrospinal fluid	0:18	Cerebrospinal fluid	0:18	Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry.					
31857120	3	83	theme	human	903:907	arg1	gangliosidome					913:925	human CSF gangliosidome	903:925	human CSF gangliosidome	903:925	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	4	84	theme	MS	1014:1015	arg1	separation					1017:1026	IMS MS separation	1010:1026	IMS MS separation	1010:1026	IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain.					
31857120	5	85	theme	carbon	1275:1280	arg1	atoms					1282:1286	carbon atoms	1275:1286	carbon atoms	1275:1286	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	3	86	theme	high	675:678	arg1	performance					680:690	recently the high performance	662:690	recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs	662:810	Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides.					
31857120	1	87	theme	tissue	223:228	arg1	biopsies					230:237	tissue biopsies	223:237	tissue biopsies	223:237	The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF.					
31857120	5	88	with	comparison	1154:1163	arg1	tissue					1180:1185	the brain tissue	1170:1185	the brain tissue	1170:1185	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31857120	5	89	theme	brain	1174:1178	arg1	tissue					1180:1185	the brain tissue	1170:1185	the brain tissue	1170:1185	In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains.					
31173196	0	0	from	study	14:18	arg1	forms					83:87	two different forms	69:87	two different forms on osteogenesis in vitro	69:112	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	12	1	from	properties	1583:1592	arg1	forms					1626:1630	the two different forms in vitro	1608:1639	the two different forms in vitro	1608:1639	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	5	2	theme	freeze‑dried	479:490	arg1	samples					496:502	The freeze‑dried CGF samples	475:502	The freeze‑dried CGF samples	475:502	The freeze‑dried CGF samples were mixed with chitosan‑alginate composite hydrogels, and the mixture was lyophilized.					
31173196	8	3	theme	MC3T3‑E1	884:891	arg1	cells					893:897	MC3T3‑E1 cells	884:897	MC3T3‑E1 cells	884:897	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	9	4	theme	growth	1062:1067	arg1	TGF‑β1					1080:1085	TGF‑β1	1080:1085	TGF‑β1	1080:1085	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	4	theme	growth	1062:1067	arg1	factor β1					1069:1077	growth factor β1	1062:1077	growth factor β1 (TGF‑β1)	1062:1086	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	4	5	theme	freeze‑dried	457:468	arg1	CGF					470:472	freeze‑dried CGF	457:472	freeze‑dried CGF	457:472	CGF samples were then lyophilized and grinded into a powder, which was termed freeze‑dried CGF.					
31173196	1	6	theme	growth	146:151	arg1	factors					153:159	growth factors	146:159	growth factors	146:159	Extending the release cycle of growth factors to match the cycle of bone remodeling is difficult.					
31173196	11	7	theme	significant	1458:1468	arg1	advantages					1470:1479	significant advantages	1458:1479	significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization	1458:1549	At later stages, the sustained‑release CGF had significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization.					
31173196	11	8	contain	had	1454:1456	arg1	CGF					1450:1452	the sustained‑release CGF	1428:1452	the sustained‑release CGF	1428:1452	At later stages, the sustained‑release CGF had significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization.					
31173196	11	8	contain	had	1454:1456	arg2	advantages					1470:1479	significant advantages	1458:1479	significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization	1458:1549	At later stages, the sustained‑release CGF had significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization.					
31173196	12	9	theme	better	1656:1661	arg1	understanding					1663:1675	a better understanding	1654:1675	a better understanding of their clinical effects	1654:1701	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	8	10	theme	sustained‑release	969:985	arg1	CGF					987:989	sustained‑release CGF	969:989	sustained‑release CGF	969:989	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	10	11	theme	osteogenic	1336:1345	arg1	performance					1347:1357	better osteogenic performance	1329:1357	better osteogenic performance	1329:1357	The freeze‑dried CGF showed better osteogenic performance than the sustained‑release CGF in the early stages.					
31173196	3	12	theme	present	319:325	arg1	study					327:331	the present study	315:331	the present study	315:331	In the present study, CGF samples were prepared by centrifugation.					
31173196	9	13	theme	ELISA	1289:1293	arg1	kits					1295:1298	ELISA kits	1289:1298	ELISA kits	1289:1298	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	14	theme	endothelial	1184:1194	arg1	VEGF					1211:1214	VEGF	1211:1214	VEGF	1211:1214	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	14	theme	endothelial	1184:1194	arg1	factor					1203:1208	vascular endothelial growth factor	1175:1208	vascular endothelial growth factor (VEGF)	1175:1215	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	11	15	theme	sustained‑release	1432:1448	arg1	CGF					1450:1452	the sustained‑release CGF	1428:1452	the sustained‑release CGF	1428:1452	At later stages, the sustained‑release CGF had significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization.					
31173196	6	16	theme	sustained‑release	668:684	arg1	CGF					686:688	sustained‑release CGF	668:688	sustained‑release CGF	668:688	The result was a chitosan‑alginate composite CGF membrane, which was called sustained‑release CGF.					
31173196	0	17	theme	osteogenesis	92:103	arg1	in vitro					105:112	osteogenesis in vitro	92:112	osteogenesis in vitro	92:112	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	8	18	theme	osteogenic	859:868	arg1	expression					870:879	osteogenic expression	859:879	osteogenic expression of MC3T3‑E1 cells	859:897	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	9	19	theme	growth	1196:1201	arg1	VEGF					1211:1214	VEGF	1211:1214	VEGF	1211:1214	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	19	theme	growth	1196:1201	arg1	factor					1203:1208	vascular endothelial growth factor	1175:1208	vascular endothelial growth factor (VEGF)	1175:1215	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	1	20	theme	factors	153:159	arg1	cycle					137:141	the release cycle	125:141	the release cycle of growth factors to match the cycle of bone remodeling	125:197	Extending the release cycle of growth factors to match the cycle of bone remodeling is difficult.					
31173196	12	21	theme	CGF	1601:1603	arg1	properties					1583:1592	the biologic properties	1570:1592	the biologic properties of the CGF in the two different forms in vitro	1570:1639	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	0	22	from	effects	27:33	arg1	forms					83:87	two different forms	69:87	two different forms on osteogenesis in vitro	69:112	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	0	22	from	effects	27:33	arg1	in vitro					105:112	osteogenesis in vitro	92:112	osteogenesis in vitro	92:112	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	8	23	with	incubation	932:941	arg1	CGF					961:963	freeze‑dried CGF	948:963	freeze‑dried CGF	948:963	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	8	23	with	incubation	932:941	arg1	CGF					987:989	sustained‑release CGF	969:989	sustained‑release CGF	969:989	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	11	24	theme	later	1414:1418	arg1	stages					1420:1425	later stages	1414:1425	later stages	1414:1425	At later stages, the sustained‑release CGF had significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization.					
31173196	0	25	theme	comparative	2:12	arg1	study					14:18	A comparative study	0:18	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.	0:113	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	5	26	theme	chitosan‑alginate	520:536	arg1	hydrogels					548:556	chitosan‑alginate composite hydrogels	520:556	chitosan‑alginate composite hydrogels	520:556	The freeze‑dried CGF samples were mixed with chitosan‑alginate composite hydrogels, and the mixture was lyophilized.					
31173196	2	27	theme	growth	275:280	arg1	factors					282:288	growth factors	275:288	growth factors	275:288	When using concentrated growth factors (CGFs), the release of growth factors is excessively rapid.					
31173196	9	28	theme	insulin‑like	1089:1100	arg1	factor‑1					1109:1116	insulin‑like growth factor‑1	1089:1116	insulin‑like growth factor‑1 (IGF‑1)	1089:1124	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	28	theme	insulin‑like	1089:1100	arg1	IGF‑1					1119:1123	IGF‑1	1119:1123	IGF‑1	1119:1123	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	12	29	theme	biologic	1574:1581	arg1	properties					1583:1592	the biologic properties	1570:1592	the biologic properties of the CGF in the two different forms in vitro	1570:1639	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	5	30	theme	composite	538:546	arg1	hydrogels					548:556	chitosan‑alginate composite hydrogels	520:556	chitosan‑alginate composite hydrogels	520:556	The freeze‑dried CGF samples were mixed with chitosan‑alginate composite hydrogels, and the mixture was lyophilized.					
31173196	9	31	theme	experimental	1231:1242	arg1	groups					1244:1249	these two experimental groups	1221:1249	these two experimental groups at different times	1221:1268	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	8	32	theme	cells	893:897	arg1	expression					870:879	osteogenic expression	859:879	osteogenic expression of MC3T3‑E1 cells	859:897	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	8	32	theme	cells	893:897	arg1	proliferation					841:853	proliferation	841:853	proliferation	841:853	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	9	33	link	platelet‑derived	1127:1142	arg1	PDGF‑AB					1162:1168	PDGF‑AB	1162:1168	PDGF‑AB	1162:1168	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	33	link	platelet‑derived	1127:1142	arg1	factor‑AB					1151:1159	platelet‑derived growth factor‑AB	1127:1159	platelet‑derived growth factor‑AB (PDGF‑AB)	1127:1169	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	12	34	from	forms	1626:1630	arg1	properties					1583:1592	the biologic properties	1570:1592	the biologic properties of the CGF in the two different forms in vitro	1570:1639	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	10	35	theme	sustained‑release	1368:1384	arg1	CGF					1386:1388	the sustained‑release CGF	1364:1388	the sustained‑release CGF in the early stages	1364:1408	The freeze‑dried CGF showed better osteogenic performance than the sustained‑release CGF in the early stages.					
31173196	9	36	theme	vascular	1175:1182	arg1	VEGF					1211:1214	VEGF	1211:1214	VEGF	1211:1214	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	36	theme	vascular	1175:1182	arg1	factor					1203:1208	vascular endothelial growth factor	1175:1208	vascular endothelial growth factor (VEGF)	1175:1215	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	7	37	theme	effective	813:821	arg1	osteogenesis					823:834	effective osteogenesis	813:834	effective osteogenesis	813:834	This study investigated whether freeze‑dried CGF in a chitosan‑alginate composite gel can release CGF steadily to achieve effective osteogenesis.					
31173196	10	38	theme	freeze‑dried	1305:1316	arg1	CGF					1318:1320	The freeze‑dried CGF	1301:1320	The freeze‑dried CGF	1301:1320	The freeze‑dried CGF showed better osteogenic performance than the sustained‑release CGF in the early stages.					
31173196	9	39	theme	platelet‑derived	1127:1142	arg1	PDGF‑AB					1162:1168	PDGF‑AB	1162:1168	PDGF‑AB	1162:1168	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	39	theme	platelet‑derived	1127:1142	arg1	factor‑AB					1151:1159	platelet‑derived growth factor‑AB	1127:1159	platelet‑derived growth factor‑AB (PDGF‑AB)	1127:1169	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	11	40	from	CGF	1499:1501	arg1	terms					1506:1510	terms	1506:1510	terms of promoting osteogenic mineralization	1506:1549	At later stages, the sustained‑release CGF had significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization.					
31173196	6	41	theme	CGF	637:639	arg1	membrane					641:648	a chitosan‑alginate composite CGF membrane	607:648	a chitosan‑alginate composite CGF membrane	607:648	The result was a chitosan‑alginate composite CGF membrane, which was called sustained‑release CGF.					
31173196	6	41	theme	CGF	637:639	arg1	result					596:601	The result	592:601	The result	592:601	The result was a chitosan‑alginate composite CGF membrane, which was called sustained‑release CGF.					
31173196	9	42	theme	growth	1102:1107	arg1	factor‑1					1109:1116	insulin‑like growth factor‑1	1089:1116	insulin‑like growth factor‑1 (IGF‑1)	1089:1124	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	42	theme	growth	1102:1107	arg1	IGF‑1					1119:1123	IGF‑1	1119:1123	IGF‑1	1119:1123	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	0	43	theme	effects	27:33	arg1	study					14:18	A comparative study	0:18	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.	0:113	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	12	44	theme	different	1616:1624	arg1	forms					1626:1630	the two different forms in vitro	1608:1639	the two different forms in vitro	1608:1639	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	6	45	theme	composite	627:635	arg1	membrane					641:648	a chitosan‑alginate composite CGF membrane	607:648	a chitosan‑alginate composite CGF membrane	607:648	The result was a chitosan‑alginate composite CGF membrane, which was called sustained‑release CGF.					
31173196	6	45	theme	composite	627:635	arg1	result					596:601	The result	592:601	The result	592:601	The result was a chitosan‑alginate composite CGF membrane, which was called sustained‑release CGF.					
31173196	1	46	theme	bone	183:186	arg1	remodeling					188:197	bone remodeling	183:197	bone remodeling	183:197	Extending the release cycle of growth factors to match the cycle of bone remodeling is difficult.					
31173196	8	47	from	incubation	932:941	arg1	supernatants					914:925	the supernatants	910:925	the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF	910:989	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	7	48	theme	chitosan‑alginate	745:761	arg1	gel					773:775	a chitosan‑alginate composite gel	743:775	a chitosan‑alginate composite gel	743:775	This study investigated whether freeze‑dried CGF in a chitosan‑alginate composite gel can release CGF steadily to achieve effective osteogenesis.					
31173196	12	49	dep	forms	1626:1630	arg1	in vitro					1632:1639	in vitro	1632:1639	in vitro	1632:1639	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	9	50	from	times	1264:1268	arg1	groups					1244:1249	these two experimental groups	1221:1249	these two experimental groups at different times	1221:1268	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	50	from	times	1264:1268	arg1	concentrations					1011:1024	The concentrations	1007:1024	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times	1007:1268	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	10	51	theme	better	1329:1334	arg1	performance					1347:1357	better osteogenic performance	1329:1357	better osteogenic performance	1329:1357	The freeze‑dried CGF showed better osteogenic performance than the sustained‑release CGF in the early stages.					
31173196	5	52	theme	CGF	492:494	arg1	samples					496:502	The freeze‑dried CGF samples	475:502	The freeze‑dried CGF samples	475:502	The freeze‑dried CGF samples were mixed with chitosan‑alginate composite hydrogels, and the mixture was lyophilized.					
31173196	1	53	theme	remodeling	188:197	arg1	cycle					174:178	the cycle	170:178	the cycle of bone remodeling	170:197	Extending the release cycle of growth factors to match the cycle of bone remodeling is difficult.					
31173196	0	54	theme	concentrated	38:49	arg1	factors					58:64	concentrated growth factors	38:64	concentrated growth factors	38:64	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	7	55	theme	composite	763:771	arg1	gel					773:775	a chitosan‑alginate composite gel	743:775	a chitosan‑alginate composite gel	743:775	This study investigated whether freeze‑dried CGF in a chitosan‑alginate composite gel can release CGF steadily to achieve effective osteogenesis.					
31173196	12	56	theme	effects	1695:1701	arg1	understanding					1663:1675	a better understanding	1654:1675	a better understanding of their clinical effects	1654:1701	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	10	57	from	CGF	1386:1388	arg1	stages					1403:1408	the early stages	1393:1408	the early stages	1393:1408	The freeze‑dried CGF showed better osteogenic performance than the sustained‑release CGF in the early stages.					
31173196	12	58	from	CGF	1601:1603	arg1	forms					1626:1630	the two different forms in vitro	1608:1639	the two different forms in vitro	1608:1639	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	4	59	theme	CGF	379:381	arg1	samples					383:389	CGF samples	379:389	CGF samples	379:389	CGF samples were then lyophilized and grinded into a powder, which was termed freeze‑dried CGF.					
31173196	8	60	dep	proliferation	841:853	arg1	The					837:839	The	837:839	The	837:839	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	6	61	theme	chitosan‑alginate	609:625	arg1	membrane					641:648	a chitosan‑alginate composite CGF membrane	607:648	a chitosan‑alginate composite CGF membrane	607:648	The result was a chitosan‑alginate composite CGF membrane, which was called sustained‑release CGF.					
31173196	6	61	theme	chitosan‑alginate	609:625	arg1	result					596:601	The result	592:601	The result	592:601	The result was a chitosan‑alginate composite CGF membrane, which was called sustained‑release CGF.					
31173196	10	62	theme	early	1397:1401	arg1	stages					1403:1408	the early stages	1393:1408	the early stages	1393:1408	The freeze‑dried CGF showed better osteogenic performance than the sustained‑release CGF in the early stages.					
31173196	11	63	theme	osteogenic	1525:1534	arg1	mineralization					1536:1549	osteogenic mineralization	1525:1549	osteogenic mineralization	1525:1549	At later stages, the sustained‑release CGF had significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization.					
31173196	9	64	theme	growth	1144:1149	arg1	PDGF‑AB					1162:1168	PDGF‑AB	1162:1168	PDGF‑AB	1162:1168	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	64	theme	growth	1144:1149	arg1	factor‑AB					1151:1159	platelet‑derived growth factor‑AB	1127:1159	platelet‑derived growth factor‑AB (PDGF‑AB)	1127:1169	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	65	theme	growth	1033:1038	arg1	factors					1040:1046	the growth factors	1029:1046	the growth factors	1029:1046	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	7	66	theme	freeze‑dried	723:734	arg1	CGF					736:738	freeze‑dried CGF	723:738	freeze‑dried CGF in a chitosan‑alginate composite gel	723:775	This study investigated whether freeze‑dried CGF in a chitosan‑alginate composite gel can release CGF steadily to achieve effective osteogenesis.					
31173196	7	67	from	CGF	736:738	arg1	gel					773:775	a chitosan‑alginate composite gel	743:775	a chitosan‑alginate composite gel	743:775	This study investigated whether freeze‑dried CGF in a chitosan‑alginate composite gel can release CGF steadily to achieve effective osteogenesis.					
31173196	0	68	from	forms	83:87	arg1	study					14:18	A comparative study	0:18	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.	0:113	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	0	68	from	forms	83:87	arg1	in vitro					105:112	osteogenesis in vitro	92:112	osteogenesis in vitro	92:112	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	2	69	theme	growth	237:242	arg1	CGFs					253:256	CGFs	253:256	CGFs	253:256	When using concentrated growth factors (CGFs), the release of growth factors is excessively rapid.					
31173196	2	69	theme	growth	237:242	arg1	factors 					244:251	concentrated growth factors 	224:251	concentrated growth factors (CGFs)	224:257	When using concentrated growth factors (CGFs), the release of growth factors is excessively rapid.					
31173196	8	70	theme	freeze‑dried	948:959	arg1	CGF					961:963	freeze‑dried CGF	948:963	freeze‑dried CGF	948:963	The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated.					
31173196	2	71	theme	factors	282:288	arg1	release					264:270	the release	260:270	the release of growth factors	260:288	When using concentrated growth factors (CGFs), the release of growth factors is excessively rapid.					
31173196	2	71	theme	factors	282:288	arg1	rapid					305:309	rapid	305:309	rapid	305:309	When using concentrated growth factors (CGFs), the release of growth factors is excessively rapid.					
31173196	9	72	theme	factors	1040:1046	arg1	concentrations					1011:1024	The concentrations	1007:1024	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times	1007:1268	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	0	73	theme	factors	58:64	arg1	effects					27:33	the effects	23:33	the effects of concentrated growth factors in two different forms on osteogenesis in vitro	23:112	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	9	74	theme	different	1254:1262	arg1	times					1264:1268	different times	1254:1268	different times	1254:1268	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	2	75	theme	concentrated	224:235	arg1	CGFs					253:256	CGFs	253:256	CGFs	253:256	When using concentrated growth factors (CGFs), the release of growth factors is excessively rapid.					
31173196	2	75	theme	concentrated	224:235	arg1	factors 					244:251	concentrated growth factors 	224:251	concentrated growth factors (CGFs)	224:257	When using concentrated growth factors (CGFs), the release of growth factors is excessively rapid.					
31173196	1	76	theme	release	129:135	arg1	cycle					137:141	the release cycle	125:141	the release cycle of growth factors to match the cycle of bone remodeling	125:197	Extending the release cycle of growth factors to match the cycle of bone remodeling is difficult.					
31173196	0	77	theme	growth	51:56	arg1	factors					58:64	concentrated growth factors	38:64	concentrated growth factors	38:64	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	9	78	from	concentrations	1011:1024	arg1	groups					1244:1249	these two experimental groups	1221:1249	these two experimental groups at different times	1221:1268	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	9	78	from	concentrations	1011:1024	arg1	times					1264:1268	different times	1254:1268	different times	1254:1268	The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits.					
31173196	0	79	from	in vitro	105:112	arg1	effects					27:33	the effects	23:33	the effects of concentrated growth factors in two different forms on osteogenesis in vitro	23:112	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
31173196	11	80	theme	freeze‑dried	1486:1497	arg1	CGF					1499:1501	freeze‑dried CGF	1486:1501	freeze‑dried CGF in terms of promoting osteogenic mineralization	1486:1549	At later stages, the sustained‑release CGF had significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization.					
31173196	3	81	theme	CGF	334:336	arg1	samples					338:344	CGF samples	334:344	CGF samples	334:344	In the present study, CGF samples were prepared by centrifugation.					
31173196	12	82	theme	clinical	1686:1693	arg1	effects					1695:1701	their clinical effects	1680:1701	their clinical effects	1680:1701	By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.					
31173196	0	83	theme	different	73:81	arg1	forms					83:87	two different forms	69:87	two different forms on osteogenesis in vitro	69:112	A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.					
30347384	12	0	theme	new	2056:2058	arg1	matrices					2060:2067	new matrices	2056:2067	new matrices	2056:2067	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	9	1	theme	contact	1739:1745	arg1	time					1747:1750	30 s contact time	1734:1750	30 s contact time	1734:1750	The strongest adhesion was observed between Col IV films, and between Col IV and LN-521 films after 30 s contact time.					
30347384	1	2	theme	biomedical	237:246	arg1	applications					248:259	various biomedical applications	229:259	various biomedical applications	229:259	Biomaterials of different nature have been and are widely studied for various biomedical applications.					
30347384	10	3	theme	LN-521	1838:1843	arg1	films					1845:1849	LN-521 films	1838:1849	LN-521 films	1838:1849	In contrast, low adhesion was measured between CNF films, as well as between CNF and LN-521 films.					
30347384	10	4	theme	CNF	1800:1802	arg1	films					1804:1808	CNF films	1800:1808	CNF films	1800:1808	In contrast, low adhesion was measured between CNF films, as well as between CNF and LN-521 films.					
30347384	12	5	theme	biological	2007:2016	arg1	systems					2018:2024	biological systems	2007:2024	biological systems	2007:2024	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	9	6	theme	30 s	1734:1737	arg1	time					1747:1750	30 s contact time	1734:1750	30 s contact time	1734:1750	The strongest adhesion was observed between Col IV films, and between Col IV and LN-521 films after 30 s contact time.					
30347384	12	7	theme	matrices	2060:2067	arg1	design					2046:2051	the design	2042:2051	the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications	2042:2169	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	5	8	theme	nature	955:960	arg1	cross-interactions					791:808	cross-interactions	791:808	cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is	791:926	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	5	8	theme	nature	955:960	arg1	biomaterials					929:940	biomaterials	929:940	biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering	929:1112	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	2	9	theme	biomaterial	277:287	arg1	assemblies					289:298	biomaterial assemblies	277:298	biomaterial assemblies	277:298	In many cases, biomaterial assemblies are designed to mimic biological systems.					
30347384	7	10	with	substrates	1453:1462	arg1	films					1526:1530	homogeneous biomaterial films	1502:1530	homogeneous biomaterial films	1502:1530	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	6	11	theme	probe	1317:1321	arg1	technique					1323:1331	the colloidal probe technique	1303:1331	the colloidal probe technique	1303:1331	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	9	12	theme	strongest	1638:1646	arg1	adhesion					1648:1655	The strongest adhesion	1634:1655	The strongest adhesion	1634:1655	The strongest adhesion was observed between Col IV films, and between Col IV and LN-521 films after 30 s contact time.					
30347384	12	13	theme	medical	2150:2156	arg1	applications					2158:2169	diverse biological or medical applications	2128:2169	diverse biological or medical applications	2128:2169	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	6	14	theme	colloidal	1307:1315	arg1	technique					1323:1331	the colloidal probe technique	1303:1331	the colloidal probe technique	1303:1331	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	12	15	theme	structure	1994:2002	arg1	understanding					1973:1985	our understanding	1969:1985	our understanding of the structure of biological systems	1969:2024	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	6	16	theme	flat	1207:1210	arg1	substrates					1212:1221	flat substrates	1207:1221	flat substrates	1207:1221	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	7	17	dep	methods	1344:1350	arg1	methods					1344:1350	Different methods	1334:1350	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption)	1334:1410	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	7	17	dep	methods	1344:1350	arg1	spin-coating					1383:1394	spin-coating	1383:1394	spin-coating	1383:1394	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	7	17	dep	methods	1344:1350	arg1	adsorption					1400:1409	adsorption	1400:1409	adsorption	1400:1409	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	7	17	dep	methods	1344:1350	arg1	deposition					1371:1380	Langmuir-Schaefer deposition	1353:1380	Langmuir-Schaefer deposition	1353:1380	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	1	18	theme	different	175:183	arg1	nature					185:190	different nature	175:190	different nature	175:190	Biomaterials of different nature have been and are widely studied for various biomedical applications.					
30347384	5	19	theme	systematic	744:753	arg1	analysis					769:776	a systematic, quantitative analysis	742:776	analysis	769:776	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	5	20	theme	extracellular	1023:1035	arg1	matrices					1037:1044	extracellular matrices	1023:1044	extracellular matrices	1023:1044	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	5	20	theme	extracellular	1023:1035	arg1	systems					1008:1014	biological systems	997:1014	biological systems (e.g., extracellular matrices)	997:1045	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	2	21	theme	many	265:268	arg1	cases					270:274	many cases	265:274	many cases	265:274	In many cases, biomaterial assemblies are designed to mimic biological systems.					
30347384	5	22	theme	collagen	847:854	arg1	films					818:822	films	818:822	films	818:822	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	12	23	theme	diverse	2128:2134	arg1	applications					2158:2169	diverse biological or medical applications	2128:2169	diverse biological or medical applications	2128:2169	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	7	24	with	microparticles	1482:1495	arg1	films					1526:1530	homogeneous biomaterial films	1502:1530	homogeneous biomaterial films	1502:1530	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	3	25	theme	quantitative	425:436	arg1	information					438:448	not much quantitative information	416:448	not much quantitative information on the molecular level interactions between different biomaterials	416:515	Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available.					
30347384	3	26	theme	many	402:405	arg1	aspects					407:413	many aspects	402:413	many aspects	402:413	Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available.					
30347384	6	27	theme	spherical	1178:1186	arg1	microparticles					1188:1201	biomaterials-coated spherical microparticles	1158:1201	biomaterials-coated spherical microparticles	1158:1201	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	9	28	theme	LN-521	1715:1720	arg1	films					1722:1726	LN-521 films	1715:1726	LN-521 films	1715:1726	The strongest adhesion was observed between Col IV films, and between Col IV and LN-521 films after 30 s contact time.					
30347384	7	29	theme	spherical	1472:1480	arg1	microparticles					1482:1495	the spherical microparticles	1468:1495	the spherical microparticles with homogeneous biomaterial films	1468:1530	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	1	30	theme	nature	185:190	arg1	Biomaterials					159:170	Biomaterials	159:170	Biomaterials of different nature	159:190	Biomaterials of different nature have been and are widely studied for various biomedical applications.					
30347384	5	31	theme	collagen	827:834	arg1	films					818:822	films	818:822	films	818:822	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	11	32	theme	collagen	1898:1905	arg1	films					1907:1911	collagen films	1898:1911	collagen films (especially Col I)	1898:1930	Nevertheless, a good adhesion between CNF and collagen films (especially Col I) was observed.					
30347384	9	33	theme	Col	1678:1680	arg1	films					1685:1689	Col IV films	1678:1689	Col IV films	1678:1689	The strongest adhesion was observed between Col IV films, and between Col IV and LN-521 films after 30 s contact time.					
30347384	8	34	theme	biomaterials	1554:1565	arg1	films					1567:1571	biomaterials films	1554:1571	biomaterials films	1554:1571	The adhesion between biomaterials films increased with the time that the films were kept in contact.					
30347384	5	35	theme	cellulose	891:899	arg1	CNF					914:916	CNF	914:916	CNF	914:916	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	5	35	theme	cellulose	891:899	arg1	nanofibrils					901:911	cellulose nanofibrils	891:911	cellulose nanofibrils (CNF)	891:917	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	10	36	theme	low	1766:1768	arg1	adhesion					1770:1777	low adhesion	1766:1777	low adhesion	1766:1777	In contrast, low adhesion was measured between CNF films, as well as between CNF and LN-521 films.					
30347384	11	37	dep	films	1907:1911	arg1	Col					1925:1927	Col	1925:1927	Col	1925:1927	Nevertheless, a good adhesion between CNF and collagen films (especially Col I) was observed.					
30347384	5	38	theme	tissue	1095:1100	arg1	engineering					1102:1112	tissue engineering	1095:1112	tissue engineering	1095:1112	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	0	39	theme	cellulose	104:112	arg1	nanofibrils					114:124	cellulose nanofibrils	104:124	cellulose nanofibrils	104:124	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	7	40	theme	biomaterial	1514:1524	arg1	films					1526:1530	homogeneous biomaterial films	1502:1530	homogeneous biomaterial films	1502:1530	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	5	41	theme	nanofibrils	901:911	arg1	films					818:822	films	818:822	films	818:822	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	11	42	theme	good	1868:1871	arg1	adhesion					1873:1880	a good adhesion	1866:1880	a good adhesion between CNF and collagen films (especially Col I)	1866:1930	Nevertheless, a good adhesion between CNF and collagen films (especially Col I) was observed.					
30347384	5	43	theme	self-	781:785	arg1	analysis					769:776	a systematic, quantitative analysis	742:776	analysis	769:776	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	6	44	theme	force	1282:1286	arg1	microscope					1288:1297	an atomic force microscope	1272:1297	an atomic force microscope	1272:1297	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	4	45	theme	other	647:651	arg1	hand					653:656	the other hand	643:656	the other hand	643:656	That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications.					
30347384	6	46	theme	biomaterials-coated	1158:1176	arg1	microparticles					1188:1201	biomaterials-coated spherical microparticles	1158:1201	biomaterials-coated spherical microparticles	1158:1201	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	3	47	theme	molecular	457:465	arg1	interactions					473:484	the molecular level interactions	453:484	the molecular level interactions between different biomaterials	453:515	Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available.					
30347384	0	48	theme	biomimetic	37:46	arg1	collagen					75:82	collagen IV	75:85	collagen IV	75:85	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	0	48	theme	biomimetic	37:46	arg1	biomaterials					48:59	biomimetic biomaterials	37:59	biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils -	37:126	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	0	48	theme	biomimetic	37:46	arg1	laminin					88:94	laminin 521	88:98	laminin 521	88:98	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	0	48	theme	biomimetic	37:46	arg1	collagen					63:70	collagen I	63:72	collagen I	63:72	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	12	49	theme	biological	2136:2145	arg1	applications					2158:2169	diverse biological or medical applications	2128:2169	diverse biological or medical applications	2128:2169	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	6	50	theme	atomic	1275:1280	arg1	microscope					1288:1297	an atomic force microscope	1272:1297	an atomic force microscope	1272:1297	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	4	51	dep	understand	587:596	arg1	develop					662:668	develop	662:668	to develop new composite biomaterials for special applications	659:720	That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications.					
30347384	5	52	theme	different	945:953	arg1	nature					955:960	different nature	945:960	different nature	945:960	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	3	53	theme	level	467:471	arg1	interactions					473:484	the molecular level interactions	453:484	the molecular level interactions between different biomaterials	453:515	Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available.					
30347384	5	54	theme	cross-interactions	791:808	arg1	analysis					769:776	a systematic, quantitative analysis	742:776	analysis	769:776	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	5	55	dep	matrices	1037:1044	arg1	e.g.					1017:1020	e.g.	1017:1020	e.g.	1017:1020	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	0	56	theme	probe	141:145	arg1	microscopy					147:156	colloidal probe microscopy	131:156	colloidal probe microscopy	131:156	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	7	57	theme	homogeneous	1502:1512	arg1	films					1526:1530	homogeneous biomaterial films	1502:1530	homogeneous biomaterial films	1502:1530	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	0	58	dep	biomaterials	48:59	arg1	collagen					75:82	collagen IV	75:85	collagen IV	75:85	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	0	58	dep	biomaterials	48:59	arg1	laminin					88:94	laminin 521	88:98	laminin 521	88:98	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	0	58	dep	biomaterials	48:59	arg1	biomaterials					48:59	biomimetic biomaterials	37:59	biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils -	37:126	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	0	58	dep	biomaterials	48:59	arg1	nanofibrils					114:124	cellulose nanofibrils	104:124	cellulose nanofibrils	104:124	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	0	58	dep	biomaterials	48:59	arg1	collagen					63:70	collagen I	63:72	collagen I	63:72	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	8	59	dep	time	1592:1595	arg1	kept					1617:1620	kept	1617:1620	were kept in contact	1612:1631	The adhesion between biomaterials films increased with the time that the films were kept in contact.					
30347384	5	60	theme	laminin	869:875	arg1	films					818:822	films	818:822	films	818:822	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	5	61	theme	3D	1075:1076	arg1	culture					1083:1089	3D cell culture	1075:1089	3D cell culture	1075:1089	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	0	62	theme	colloidal	131:139	arg1	microscopy					147:156	colloidal probe microscopy	131:156	colloidal probe microscopy	131:156	Quantifying the interactions between biomimetic biomaterials - collagen I, collagen IV, laminin 521 and cellulose nanofibrils - by colloidal probe microscopy.					
30347384	5	63	theme	structure	966:974	arg1	cross-interactions					791:808	cross-interactions	791:808	cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is	791:926	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	5	63	theme	structure	966:974	arg1	biomaterials					929:940	biomaterials	929:940	biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering	929:1112	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	7	64	theme	Different	1334:1342	arg1	adsorption					1400:1409	adsorption	1400:1409	adsorption	1400:1409	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	7	64	theme	Different	1334:1342	arg1	spin-coating					1383:1394	spin-coating	1383:1394	spin-coating	1383:1394	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	7	64	theme	Different	1334:1342	arg1	methods					1344:1350	Different methods	1334:1350	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption)	1334:1410	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	7	64	theme	Different	1334:1342	arg1	deposition					1371:1380	Langmuir-Schaefer deposition	1353:1380	Langmuir-Schaefer deposition	1353:1380	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	4	65	theme	special	701:707	arg1	applications					709:720	special applications	701:720	special applications	701:720	That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications.					
30347384	5	66	theme	cell	1078:1081	arg1	culture					1083:1089	3D cell culture	1075:1089	3D cell culture	1075:1089	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	12	67	theme	different	2088:2096	arg1	biomaterials					2098:2109	different biomaterials	2088:2109	different biomaterials	2088:2109	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	2	68	theme	biological	322:331	arg1	systems					333:339	biological systems	322:339	biological systems	322:339	In many cases, biomaterial assemblies are designed to mimic biological systems.					
30347384	7	69	theme	Langmuir-Schaefer	1353:1369	arg1	methods					1344:1350	Different methods	1334:1350	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption)	1334:1410	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	7	69	theme	Langmuir-Schaefer	1353:1369	arg1	deposition					1371:1380	Langmuir-Schaefer deposition	1353:1380	Langmuir-Schaefer deposition	1353:1380	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	4	70	theme	biological	616:625	arg1	systems					627:633	biological systems	616:633	biological systems	616:633	That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications.					
30347384	6	71	theme	phosphate-buffered	1240:1257	arg1	saline					1259:1264	phosphate-buffered saline	1240:1264	phosphate-buffered saline using an atomic force microscope and the colloidal probe technique	1240:1331	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	12	72	theme	scaffolds	2072:2080	arg1	design					2046:2051	the design	2042:2051	the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications	2042:2169	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	6	73	theme	surface	1122:1128	arg1	forces					1130:1135	Direct surface forces	1115:1135	Direct surface forces	1115:1135	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	5	74	theme	biological	997:1006	arg1	matrices					1037:1044	extracellular matrices	1023:1044	extracellular matrices	1023:1044	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	5	74	theme	biological	997:1006	arg1	systems					1008:1014	biological systems	997:1014	biological systems (e.g., extracellular matrices)	997:1045	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	3	75	theme	different	494:502	arg1	biomaterials					504:515	different biomaterials	494:515	different biomaterials	494:515	Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available.					
30347384	12	76	theme	systems	2018:2024	arg1	structure					1994:2002	the structure	1990:2002	the structure of biological systems	1990:2024	These results increase our understanding of the structure of biological systems and can support the design of new matrices or scaffolds where different biomaterials are combined for diverse biological or medical applications.					
30347384	6	77	theme	Direct	1115:1120	arg1	forces					1130:1135	Direct surface forces	1115:1135	Direct surface forces	1115:1135	Direct surface forces and adhesion between biomaterials-coated spherical microparticles and flat substrates were measured in phosphate-buffered saline using an atomic force microscope and the colloidal probe technique.					
30347384	3	78	from	information	438:448	arg1	interactions					473:484	the molecular level interactions	453:484	the molecular level interactions between different biomaterials	453:515	Although biomaterials have been thoroughly characterized in many aspects, not much quantitative information on the molecular level interactions between different biomaterials is available.					
30347384	4	79	theme	composite	674:682	arg1	biomaterials					684:695	new composite biomaterials	670:695	new composite biomaterials for special applications	670:720	That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications.					
30347384	5	80	theme	quantitative	756:767	arg1	analysis					769:776	a systematic, quantitative analysis	742:776	analysis	769:776	This work presents a systematic, quantitative analysis of self- and cross-interactions between films of collagen I (Col I), collagen IV (Col IV), laminin (LN-521), and cellulose nanofibrils (CNF), that is, biomaterials of different nature and structure that either exist in biological systems (e.g., extracellular matrices) or have shown potential for 3D cell culture and tissue engineering.					
30347384	7	81	theme	flat	1448:1451	arg1	substrates					1453:1462	the flat substrates	1444:1462	the flat substrates	1444:1462	Different methods (Langmuir-Schaefer deposition, spin-coating, or adsorption) were applied to completely coat the flat substrates and the spherical microparticles with homogeneous biomaterial films.					
30347384	1	82	theme	various	229:235	arg1	applications					248:259	various biomedical applications	229:259	various biomedical applications	229:259	Biomaterials of different nature have been and are widely studied for various biomedical applications.					
30347384	4	83	theme	new	670:672	arg1	biomaterials					684:695	new composite biomaterials	670:695	new composite biomaterials for special applications	670:720	That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications.					
30347384	4	84	theme	systems	627:633	arg1	properties					602:611	the properties	598:611	the properties of biological systems	598:633	That information is very important, on the one hand, to understand the properties of biological systems and, on the other hand, to develop new composite biomaterials for special applications.					
31187295	1	0	theme	healing	162:168	arg1	success					145:151	The success	141:151	The success of wound healing	141:168	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31187295	5	1	theme	synthesized	749:759	arg1	derivative					770:779	the synthesized chitosan derivative	745:779	the synthesized chitosan derivative	745:779	Biodegradability of the synthesized chitosan derivative was tested in the presence of lysozyme and was found to be comparable with CS.					
31187295	7	2	from	soluble	1029:1035	arg1	majority					1040:1047	majority	1040:1047	majority of organic solvents	1040:1067	The CSD was found to be soluble in majority of organic solvents.					
31187295	0	3	theme	potential	80:88	arg1	candidates					90:99	potential candidates	80:99	potential candidates for healing chronic non-healing wounds	80:138	Novel chitosan derivative based composite scaffolds with enhanced angiogenesis; potential candidates for healing chronic non-healing wounds.					
31187295	6	4	theme	derivative	905:914	arg1	effect					891:896	The cytotoxicity and apoptosis effect	860:896	The cytotoxicity and apoptosis effect of new derivative	860:914	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	6	4	theme	derivative	905:914	arg1	non-toxic					994:1002	non-toxic	994:1002	non-toxic	994:1002	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	0	5	theme	healing	105:111	arg1	wounds					133:138	healing chronic non-healing wounds	105:138	healing chronic non-healing wounds	105:138	Novel chitosan derivative based composite scaffolds with enhanced angiogenesis; potential candidates for healing chronic non-healing wounds.					
31187295	6	6	theme	new	901:903	arg1	derivative					905:914	new derivative	901:914	new derivative	901:914	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	3	7	theme	chitosan	508:515	arg1	reaction					496:503	the reaction	492:503	the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF)	492:580	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	7	8	from	majority	1040:1047	arg1	soluble					1029:1035	soluble	1029:1035	soluble	1029:1035	The CSD was found to be soluble in majority of organic solvents.					
31187295	7	8	from	majority	1040:1047	arg1	CSD					1009:1011	The CSD	1005:1011	The CSD	1005:1011	The CSD was found to be soluble in majority of organic solvents.					
31187295	2	9	theme	wounded	273:279	arg1	tissues					281:287	wounded tissues	273:287	wounded tissues	273:287	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	9	10	theme	ex	1150:1151	arg1	assay					1161:1165	an ex ovo CAM assay	1147:1165	an ex ovo CAM assay	1147:1165	From an ex ovo CAM assay, it was noted that CSD stimulated the angiogenesis.					
31187295	2	11	theme	wound	341:345	arg1	healing					347:353	wound healing	341:353	wound healing	341:353	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	2	12	dep	engineered	299:308	arg1	tissue					292:297	tissue	292:297	tissue	292:297	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	0	13	theme	non-healing	121:131	arg1	wounds					133:138	healing chronic non-healing wounds	105:138	healing chronic non-healing wounds	105:138	Novel chitosan derivative based composite scaffolds with enhanced angiogenesis; potential candidates for healing chronic non-healing wounds.					
31187295	4	14	theme	covalent	700:707	arg1	connectivities					709:722	new covalent connectivities	696:722	new covalent connectivities	696:722	The synthesized material was characterized by FTIR and 13C-NMR to confirm the incorporated functional groups and new covalent connectivities.					
31187295	5	15	theme	lysozyme	811:818	arg1	presence					799:806	the presence	795:806	the presence of lysozyme	795:818	Biodegradability of the synthesized chitosan derivative was tested in the presence of lysozyme and was found to be comparable with CS.					
31187295	0	16	theme	chronic	113:119	arg1	wounds					133:138	healing chronic non-healing wounds	105:138	healing chronic non-healing wounds	105:138	Novel chitosan derivative based composite scaffolds with enhanced angiogenesis; potential candidates for healing chronic non-healing wounds.					
31187295	6	17	theme	apoptosis	881:889	arg1	effect					891:896	The cytotoxicity and apoptosis effect	860:896	The cytotoxicity and apoptosis effect of new derivative	860:914	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	6	17	theme	apoptosis	881:889	arg1	non-toxic					994:1002	non-toxic	994:1002	non-toxic	994:1002	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	9	18	theme	CAM	1157:1159	arg1	assay					1161:1165	an ex ovo CAM assay	1147:1165	an ex ovo CAM assay	1147:1165	From an ex ovo CAM assay, it was noted that CSD stimulated the angiogenesis.					
31187295	2	19	theme	grafts	371:376	arg1	rejection					358:366	rejection	358:366	rejection of grafts	358:376	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	2	19	theme	grafts	371:376	arg1	healing					347:353	wound healing	341:353	wound healing	341:353	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	0	20	theme	chitosan	6:13	arg1	derivative					15:24	Novel chitosan derivative	0:24	Novel chitosan derivative	0:24	Novel chitosan derivative based composite scaffolds with enhanced angiogenesis; potential candidates for healing chronic non-healing wounds.					
31187295	7	21	theme	organic	1052:1058	arg1	solvents					1060:1067	organic solvents	1052:1067	organic solvents	1052:1067	The CSD was found to be soluble in majority of organic solvents.					
31187295	1	22	theme	proper	187:192	arg1	growth					194:199	the proper growth	183:199	the proper growth of vascular system in time in the damaged tissues	183:249	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31187295	0	23	theme	Novel	0:4	arg1	derivative					15:24	Novel chitosan derivative	0:24	Novel chitosan derivative	0:24	Novel chitosan derivative based composite scaffolds with enhanced angiogenesis; potential candidates for healing chronic non-healing wounds.					
31187295	7	24	theme	solvents	1060:1067	arg1	majority					1040:1047	majority	1040:1047	majority of organic solvents	1040:1067	The CSD was found to be soluble in majority of organic solvents.					
31187295	2	25	theme	engineered	299:308	arg1	grafts					310:315	tissue engineered grafts	292:315	tissue engineered grafts	292:315	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	2	26	theme	rejection	358:366	arg1	failure					330:336	the failure	326:336	the failure of wound healing or rejection of grafts	326:376	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	5	27	with	comparable	840:849	arg1	CS					856:857	CS	856:857	CS	856:857	Biodegradability of the synthesized chitosan derivative was tested in the presence of lysozyme and was found to be comparable with CS.					
31187295	3	28	theme	1,3-dimethylbarbituric	522:543	arg1	acid					545:548	1,3-dimethylbarbituric acid	522:548	1,3-dimethylbarbituric acid	522:548	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	3	29	theme	organosoluble	430:442	arg1	synthesis					411:419	the synthesis	407:419	the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF)	407:580	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	6	30	theme	cytotoxicity	864:875	arg1	effect					891:896	The cytotoxicity and apoptosis effect	860:896	The cytotoxicity and apoptosis effect of new derivative	860:914	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	6	30	theme	cytotoxicity	864:875	arg1	non-toxic					994:1002	non-toxic	994:1002	non-toxic	994:1002	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	8	31	theme	composite	1121:1129	arg1	scaffolds					1131:1139	composite scaffolds	1121:1139	composite scaffolds	1121:1139	It was blended with polycaprolactone (PCL) to form composite scaffolds.					
31187295	1	32	theme	vascular	204:211	arg1	system					213:218	vascular system	204:218	vascular system in time	204:226	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31187295	3	33	with	reaction	496:503	arg1	acid					545:548	1,3-dimethylbarbituric acid	522:548	1,3-dimethylbarbituric acid	522:548	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	3	33	with	reaction	496:503	arg1	TEOF					576:579	TEOF	576:579	TEOF	576:579	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	3	33	with	reaction	496:503	arg1	triethylorthoformate					554:573	triethylorthoformate	554:573	triethylorthoformate (TEOF)	554:580	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	6	34	theme	AGS	963:965	arg1	cells					968:972	gastric adenocarcinoma (AGS) cells	939:972	gastric adenocarcinoma (AGS) cells	939:972	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	1	35	theme	system	213:218	arg1	growth					194:199	the proper growth	183:199	the proper growth of vascular system in time in the damaged tissues	183:249	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31187295	0	36	theme	composite	32:40	arg1	scaffolds					42:50	composite scaffolds	32:50	composite scaffolds	32:50	Novel chitosan derivative based composite scaffolds with enhanced angiogenesis; potential candidates for healing chronic non-healing wounds.					
31187295	1	37	from	system	213:218	arg1	time					223:226	time	223:226	time	223:226	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31187295	3	38	theme	novel	424:428	arg1	organosoluble					430:442	novel organosoluble	424:442	novel organosoluble	424:442	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	0	39	theme	enhanced	57:64	arg1	angiogenesis					66:77	enhanced angiogenesis	57:77	enhanced angiogenesis	57:77	Novel chitosan derivative based composite scaffolds with enhanced angiogenesis; potential candidates for healing chronic non-healing wounds.					
31187295	6	40	theme	adenocarcinoma	947:960	arg1	cells					968:972	gastric adenocarcinoma (AGS) cells	939:972	gastric adenocarcinoma (AGS) cells	939:972	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	3	41	theme	derivative	472:481	arg1	synthesis					411:419	the synthesis	407:419	the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF)	407:580	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	3	42	theme	present	382:388	arg1	paper					390:394	present paper	382:394	present paper	382:394	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	6	43	theme	gastric	939:945	arg1	cells					968:972	gastric adenocarcinoma (AGS) cells	939:972	gastric adenocarcinoma (AGS) cells	939:972	The cytotoxicity and apoptosis effect of new derivative was determined against gastric adenocarcinoma (AGS) cells and was found to be non-toxic.					
31187295	4	44	theme	new	696:698	arg1	connectivities					709:722	new covalent connectivities	696:722	new covalent connectivities	696:722	The synthesized material was characterized by FTIR and 13C-NMR to confirm the incorporated functional groups and new covalent connectivities.					
31187295	9	45	dep	ex	1150:1151	arg1	ovo					1153:1155	ovo	1153:1155	ovo	1153:1155	From an ex ovo CAM assay, it was noted that CSD stimulated the angiogenesis.					
31187295	1	46	from	time	223:226	arg1	growth					194:199	the proper growth	183:199	the proper growth of vascular system in time in the damaged tissues	183:249	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31187295	2	47	theme	blood	257:261	arg1	supply					263:268	Poor blood supply	252:268	Poor blood supply to wounded tissues or tissue engineered grafts	252:315	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	4	48	theme	incorporated	661:672	arg1	groups					685:690	the incorporated functional groups	657:690	the incorporated functional groups	657:690	The synthesized material was characterized by FTIR and 13C-NMR to confirm the incorporated functional groups and new covalent connectivities.					
31187295	3	49	theme	pro-angiogenic	448:461	arg1	derivative					472:481	pro-angiogenic chitosan derivative	448:481	pro-angiogenic chitosan derivative (CSD)	448:487	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	3	49	theme	pro-angiogenic	448:461	arg1	CSD					484:486	CSD	484:486	CSD	484:486	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	4	50	theme	synthesized	587:597	arg1	material					599:606	The synthesized material	583:606	The synthesized material	583:606	The synthesized material was characterized by FTIR and 13C-NMR to confirm the incorporated functional groups and new covalent connectivities.					
31187295	2	51	theme	Poor	252:255	arg1	supply					263:268	Poor blood supply	252:268	Poor blood supply to wounded tissues or tissue engineered grafts	252:315	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	1	52	theme	wound	156:160	arg1	healing					162:168	wound healing	156:168	wound healing	156:168	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31187295	5	53	theme	chitosan	761:768	arg1	derivative					770:779	the synthesized chitosan derivative	745:779	the synthesized chitosan derivative	745:779	Biodegradability of the synthesized chitosan derivative was tested in the presence of lysozyme and was found to be comparable with CS.					
31187295	1	54	from	growth	194:199	arg1	time					223:226	time	223:226	time	223:226	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31187295	1	54	from	growth	194:199	arg1	tissues					243:249	the damaged tissues	231:249	the damaged tissues	231:249	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31187295	4	55	theme	functional	674:683	arg1	groups					685:690	the incorporated functional groups	657:690	the incorporated functional groups	657:690	The synthesized material was characterized by FTIR and 13C-NMR to confirm the incorporated functional groups and new covalent connectivities.					
31187295	3	56	theme	chitosan	463:470	arg1	derivative					472:481	pro-angiogenic chitosan derivative	448:481	pro-angiogenic chitosan derivative (CSD)	448:487	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	3	56	theme	chitosan	463:470	arg1	CSD					484:486	CSD	484:486	CSD	484:486	In present paper, we report the synthesis of novel organosoluble and pro-angiogenic chitosan derivative (CSD) by the reaction of chitosan with 1,3-dimethylbarbituric acid and triethylorthoformate (TEOF).					
31187295	5	57	theme	derivative	770:779	arg1	Biodegradability					725:740	Biodegradability	725:740	Biodegradability of the synthesized chitosan derivative	725:779	Biodegradability of the synthesized chitosan derivative was tested in the presence of lysozyme and was found to be comparable with CS.					
31187295	2	58	theme	healing	347:353	arg1	failure					330:336	the failure	326:336	the failure of wound healing or rejection of grafts	326:376	Poor blood supply to wounded tissues or tissue engineered grafts leads to the failure of wound healing or rejection of grafts.					
31187295	1	59	theme	damaged	235:241	arg1	tissues					243:249	the damaged tissues	231:249	the damaged tissues	231:249	The success of wound healing depends upon the proper growth of vascular system in time in the damaged tissues.					
31252468	0	0	theme	Drug	90:93	arg1	Release					95:101	Drug Release	90:101	Drug Release	90:101	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	4	1	theme	hydrolytic	962:971	arg1	degradation					973:983	hydrolytic degradation	962:983	hydrolytic degradation	962:983	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	6	2	theme	cytokine	1304:1311	arg1	expression					1313:1322	inflammatory cytokine expression	1291:1322	inflammatory cytokine expression	1291:1322	The animal experiment reveals that Mg particles can alleviate macrophage infiltration and inflammatory cytokine expression.					
31252468	5	3	theme	release	1071:1077	arg1	study					1079:1083	In vivo release study	1063:1083	In vivo release study	1063:1083	In vivo release study further confirms the feasibility of Mg/PLLA microspheres for tailoring drug release in a physiological environment.					
31252468	8	4	theme	controlled	1553:1562	arg1	delivery					1564:1571	controlled delivery	1553:1571	controlled delivery of various therapeutic agents, especially some growth factors	1553:1633	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	1	5	dep	in	248:249	arg1	vivo					251:254	vivo	251:254	vivo	251:254	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	6	6	theme	inflammatory	1291:1302	arg1	expression					1313:1322	inflammatory cytokine expression	1291:1322	inflammatory cytokine expression	1291:1322	The animal experiment reveals that Mg particles can alleviate macrophage infiltration and inflammatory cytokine expression.					
31252468	4	7	theme	release	1045:1051	arg1	patterns					1053:1060	tunable release patterns	1037:1060	tunable release patterns	1037:1060	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	1	8	dep	concerned	202:210	arg1	whereas					379:385	whereas	379:385	whereas	379:385	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	2	9	theme	composite	496:504	arg1	system					539:544	a novel delivery system	522:544	a novel delivery system	522:544	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	2	9	theme	composite	496:504	arg1	microspheres					506:517	Mg/PLLA composite microspheres	488:517	Mg/PLLA composite microspheres	488:517	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	2	10	theme	inflammatory	641:652	arg1	response					654:661	PLLA-induced inflammatory response	628:661	PLLA-induced inflammatory response	628:661	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	2	11	theme	Mg	556:557	arg1	particles					559:567	Mg particles	556:567	Mg particles	556:567	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	4	12	theme	tunable	1037:1043	arg1	patterns					1053:1060	tunable release patterns	1037:1060	tunable release patterns	1037:1060	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	3	13	theme	microspheres	790:801	arg1	interior					768:775	the interior	764:775	the interior of composite microspheres	764:801	Morphological observation shows that multiple Mg particles are dispersed both on the surface and in the interior of composite microspheres.					
31252468	0	14	theme	Release	95:101	arg1	Alleviation					107:117	Alleviation	107:117	Alleviation of Inflammatory Response	107:142	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	0	14	theme	Release	95:101	arg1	Manipulation					74:85	Manipulation	74:85	Manipulation of Drug Release	74:101	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	3	15	theme	Mg	710:711	arg1	particles					713:721	multiple Mg particles	701:721	multiple Mg particles	701:721	Morphological observation shows that multiple Mg particles are dispersed both on the surface and in the interior of composite microspheres.					
31252468	2	16	theme	release	599:605	arg1	profile					607:613	the drug release profile	590:613	the drug release profile	590:613	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	4	17	theme	microspheres	931:942	arg1	connectivity					905:916	the internal connectivity	892:916	the internal connectivity of composite microspheres	892:942	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	8	18	theme	therapeutic	1584:1594	arg1	factors					1627:1633	some growth factors	1615:1633	some growth factors	1615:1633	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	8	18	theme	therapeutic	1584:1594	arg1	agents					1596:1601	various therapeutic agents	1576:1601	various therapeutic agents	1576:1601	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	6	19	theme	macrophage	1263:1272	arg1	infiltration					1274:1285	macrophage infiltration	1263:1285	macrophage infiltration	1263:1285	The animal experiment reveals that Mg particles can alleviate macrophage infiltration and inflammatory cytokine expression.					
31252468	1	20	theme	alkaline	429:436	arg1	products					438:445	alkaline products	429:445	alkaline products	429:445	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	7	21	theme	PLLA-induced	1452:1463	arg1	inflammation					1465:1476	PLLA-induced inflammation	1452:1476	PLLA-induced inflammation	1452:1476	These results demonstrate the availability of using biodegradable Mg particles to manipulate drug release as well as alleviate PLLA-induced inflammation.					
31252468	4	22	theme	composite	921:929	arg1	microspheres					931:942	composite microspheres	921:942	composite microspheres	921:942	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	4	23	theme	release	813:819	arg1	study					821:825	In vitro release study	804:825	In vitro release study	804:825	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	8	24	theme	agents	1596:1601	arg1	delivery					1564:1571	controlled delivery	1553:1571	controlled delivery of various therapeutic agents, especially some growth factors	1553:1633	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	0	25	theme	Magnesium-Incorporated	14:35	arg1	Poly					37:40	Magnesium-Incorporated Poly	14:40	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.	0:143	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	0	25	theme	Magnesium-Incorporated	14:35	arg1	acid					51:54	l-lactic acid	42:54	l-lactic acid	42:54	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	3	26	theme	multiple	701:708	arg1	particles					713:721	multiple Mg particles	701:721	multiple Mg particles	701:721	Morphological observation shows that multiple Mg particles are dispersed both on the surface and in the interior of composite microspheres.					
31252468	2	27	used	used	573:576	arg2	particles					559:567	Mg particles	556:567	Mg particles	556:567	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	0	28	theme	Biodegradable	0:12	arg1	Microspheres					57:68	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres	0:68	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.	0:143	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	1	29	theme	in	248:249	arg1	implantation					256:267	in vivo implantation	248:267	in vivo implantation	248:267	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	2	30	theme	PLLA-induced	628:639	arg1	response					654:661	PLLA-induced inflammatory response	628:661	PLLA-induced inflammatory response	628:661	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	0	31	theme	Response	135:142	arg1	Alleviation					107:117	Alleviation	107:117	Alleviation of Inflammatory Response	107:142	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	0	31	theme	Response	135:142	arg1	Manipulation					74:85	Manipulation	74:85	Manipulation of Drug Release	74:101	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	2	32	theme	drug	594:597	arg1	profile					607:613	the drug release profile	590:613	the drug release profile	590:613	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	8	33	from	applications	1537:1548	arg1	delivery					1564:1571	controlled delivery	1553:1571	controlled delivery of various therapeutic agents, especially some growth factors	1553:1633	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	7	34	theme	drug	1418:1421	arg1	release					1423:1429	drug release	1418:1429	drug release	1418:1429	These results demonstrate the availability of using biodegradable Mg particles to manipulate drug release as well as alleviate PLLA-induced inflammation.					
31252468	0	35	theme	Poly	37:40	arg1	Microspheres					57:68	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres	0:68	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.	0:143	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	0	36	theme	Inflammatory	122:133	arg1	Response					135:142	Inflammatory Response	122:142	Inflammatory Response	122:142	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	1	37	theme	surrounding	358:368	arg1	tissues					370:376	surrounding tissues	358:376	surrounding tissues	358:376	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	4	38	dep	In	804:805	arg1	vitro					807:811	vitro	807:811	vitro	807:811	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	5	39	theme	physiological	1174:1186	arg1	environment					1188:1198	a physiological environment	1172:1198	a physiological environment	1172:1198	In vivo release study further confirms the feasibility of Mg/PLLA microspheres for tailoring drug release in a physiological environment.					
31252468	4	40	theme	internal	896:903	arg1	connectivity					905:916	the internal connectivity	892:916	the internal connectivity of composite microspheres	892:942	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	8	41	theme	present	1483:1489	arg1	microspheres					1509:1520	The present Mg/PLLA composite microspheres	1479:1520	The present Mg/PLLA composite microspheres	1479:1520	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	6	42	theme	Mg	1236:1237	arg1	particles					1239:1247	Mg particles	1236:1247	Mg particles	1236:1247	The animal experiment reveals that Mg particles can alleviate macrophage infiltration and inflammatory cytokine expression.					
31252468	0	43	theme	l-lactic	42:49	arg1	Poly					37:40	Magnesium-Incorporated Poly	14:40	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.	0:143	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	0	43	theme	l-lactic	42:49	arg1	acid					51:54	l-lactic acid	42:54	l-lactic acid	42:54	Biodegradable Magnesium-Incorporated Poly(l-lactic acid) Microspheres for Manipulation of Drug Release and Alleviation of Inflammatory Response.					
31252468	5	44	theme	microspheres	1129:1140	arg1	feasibility					1106:1116	the feasibility	1102:1116	the feasibility of Mg/PLLA microspheres for tailoring drug release in a physiological environment	1102:1198	In vivo release study further confirms the feasibility of Mg/PLLA microspheres for tailoring drug release in a physiological environment.					
31252468	5	45	theme	In	1063:1064	arg1	study					1079:1083	In vivo release study	1063:1083	In vivo release study	1063:1083	In vivo release study further confirms the feasibility of Mg/PLLA microspheres for tailoring drug release in a physiological environment.					
31252468	8	46	theme	Mg/PLLA	1491:1497	arg1	microspheres					1509:1520	The present Mg/PLLA composite microspheres	1479:1520	The present Mg/PLLA composite microspheres	1479:1520	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	5	47	theme	drug	1156:1159	arg1	release					1161:1167	drug release	1156:1167	drug release	1156:1167	In vivo release study further confirms the feasibility of Mg/PLLA microspheres for tailoring drug release in a physiological environment.					
31252468	4	48	theme	drug	990:993	arg1	delivery					995:1002	drug delivery	990:1002	drug delivery	990:1002	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	8	49	theme	bone	1640:1643	arg1	regeneration					1645:1656	bone regeneration	1640:1656	bone regeneration	1640:1656	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	8	50	theme	composite	1499:1507	arg1	microspheres					1509:1520	The present Mg/PLLA composite microspheres	1479:1520	The present Mg/PLLA composite microspheres	1479:1520	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	2	51	theme	Mg/PLLA	488:494	arg1	system					539:544	a novel delivery system	522:544	a novel delivery system	522:544	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	2	51	theme	Mg/PLLA	488:494	arg1	microspheres					506:517	Mg/PLLA composite microspheres	488:517	Mg/PLLA composite microspheres	488:517	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	3	52	theme	Morphological	664:676	arg1	observation					678:688	Morphological observation	664:688	Morphological observation	664:688	Morphological observation shows that multiple Mg particles are dispersed both on the surface and in the interior of composite microspheres.					
31252468	2	53	theme	novel	524:528	arg1	system					539:544	a novel delivery system	522:544	a novel delivery system	522:544	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	2	53	theme	novel	524:528	arg1	microspheres					506:517	Mg/PLLA composite microspheres	488:517	Mg/PLLA composite microspheres	488:517	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	8	54	theme	potential	1527:1535	arg1	applications					1537:1548	potential applications	1527:1548	potential applications	1527:1548	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	4	55	theme	In	804:805	arg1	study					821:825	In vitro release study	804:825	In vitro release study	804:825	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	4	56	theme	Mg	857:858	arg1	contents					860:867	the Mg contents	853:867	the Mg contents	853:867	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	1	57	theme	acidic	290:295	arg1	byproducts					309:318	acidic degradation byproducts	290:318	acidic degradation byproducts	290:318	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	4	58	theme	particle	876:883	arg1	sizes					885:889	its particle sizes	872:889	its particle sizes	872:889	In vitro release study indicates that by varying the Mg contents or its particle sizes, the internal connectivity of composite microspheres is changed during hydrolytic degradation, and drug delivery can be facilely manipulated with tunable release patterns.					
31252468	7	59	theme	Mg	1391:1392	arg1	particles					1394:1402	biodegradable Mg particles	1377:1402	biodegradable Mg particles	1377:1402	These results demonstrate the availability of using biodegradable Mg particles to manipulate drug release as well as alleviate PLLA-induced inflammation.					
31252468	6	60	theme	animal	1205:1210	arg1	experiment					1212:1221	The animal experiment	1201:1221	The animal experiment	1201:1221	The animal experiment reveals that Mg particles can alleviate macrophage infiltration and inflammatory cytokine expression.					
31252468	5	61	dep	In	1063:1064	arg1	vivo					1066:1069	vivo	1066:1069	vivo	1066:1069	In vivo release study further confirms the feasibility of Mg/PLLA microspheres for tailoring drug release in a physiological environment.					
31252468	2	62	theme	delivery	530:537	arg1	system					539:544	a novel delivery system	522:544	a novel delivery system	522:544	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	2	62	theme	delivery	530:537	arg1	microspheres					506:517	Mg/PLLA composite microspheres	488:517	Mg/PLLA composite microspheres	488:517	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	8	63	theme	growth	1620:1625	arg1	factors					1627:1633	some growth factors	1615:1633	some growth factors	1615:1633	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	8	63	theme	growth	1620:1625	arg1	agents					1596:1601	various therapeutic agents	1576:1601	various therapeutic agents	1576:1601	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	8	64	contain	have	1522:1525	arg1	microspheres					1509:1520	The present Mg/PLLA composite microspheres	1479:1520	The present Mg/PLLA composite microspheres	1479:1520	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	8	64	contain	have	1522:1525	arg2	applications					1537:1548	potential applications	1527:1548	potential applications	1527:1548	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	3	65	theme	composite	780:788	arg1	microspheres					790:801	composite microspheres	780:801	composite microspheres	780:801	Morphological observation shows that multiple Mg particles are dispersed both on the surface and in the interior of composite microspheres.					
31252468	8	66	theme	various	1576:1582	arg1	factors					1627:1633	some growth factors	1615:1633	some growth factors	1615:1633	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	8	66	theme	various	1576:1582	arg1	agents					1596:1601	various therapeutic agents	1576:1601	various therapeutic agents	1576:1601	The present Mg/PLLA composite microspheres have potential applications in controlled delivery of various therapeutic agents, especially some growth factors, for bone regeneration.					
31252468	1	67	theme	fast	401:404	arg1	latter					391:396	latter	391:396	latter	391:396	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	1	67	theme	fast	401:404	arg1	corroded					406:413	fast corroded	401:413	fast corroded	401:413	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	5	68	theme	Mg/PLLA	1121:1127	arg1	microspheres					1129:1140	Mg/PLLA microspheres	1121:1140	Mg/PLLA microspheres	1121:1140	In vivo release study further confirms the feasibility of Mg/PLLA microspheres for tailoring drug release in a physiological environment.					
31252468	2	69	theme	study	468:472	arg1	purpose					452:458	The purpose	448:458	The purpose of this study	448:472	The purpose of this study is to develop Mg/PLLA composite microspheres as a novel delivery system, in which Mg particles are used to regulate the drug release profile and suppress PLLA-induced inflammatory response.					
31252468	1	70	theme	degradation	297:307	arg1	byproducts					309:318	acidic degradation byproducts	290:318	acidic degradation byproducts	290:318	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	7	71	theme	biodegradable	1377:1389	arg1	particles					1394:1402	biodegradable Mg particles	1377:1402	biodegradable Mg particles	1377:1402	These results demonstrate the availability of using biodegradable Mg particles to manipulate drug release as well as alleviate PLLA-induced inflammation.					
31252468	1	72	theme	l-lactic	150:157	arg1	Poly					145:148	Poly	145:148	Poly(l-lactic acid) (PLLA)	145:170	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	1	72	theme	l-lactic	150:157	arg1	acid					159:162	l-lactic acid	150:162	l-lactic acid	150:162	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31252468	1	73	theme	biodegradable	212:224	arg1	materials					226:234	biodegradable materials	212:234	biodegradable materials	212:234	Poly(l-lactic acid) (PLLA) and magnesium (Mg) are widely concerned biodegradable materials, but during in vivo implantation, the former produces acidic degradation byproducts and can easily induce inflammation in surrounding tissues, whereas the latter is fast corroded and generates alkaline products.					
31147012	9	0	theme	staining	1729:1736	arg1	results					1705:1711	the results	1701:1711	the results of alizarin red staining	1701:1736	According to the results of alizarin red staining, the calcified nodules were dyed red, which reveal that this material can promote bone formation.					
31147012	7	1	theme	outstanding	1470:1480	arg1	biocompatibility					1482:1497	outstanding biocompatibility	1470:1497	outstanding biocompatibility with VECs cells	1470:1513	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	8	2	theme	animal	1520:1525	arg1	experiments					1527:1537	The animal experiments	1516:1537	The animal experiments	1516:1537	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	6	3	theme	stem	988:991	arg1	BMSCs					1000:1004	BMSCs	1000:1004	BMSCs	1000:1004	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	6	3	theme	stem	988:991	arg1	cells					993:997	Bone marrow mesenchymal stem cells	964:997	Bone marrow mesenchymal stem cells (BMSCs)	964:1005	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	6	4	theme	dye	1264:1266	arg1	results					1241:1247	the results	1237:1247	the results of fluorescent dye	1237:1266	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	1	5	from	application	322:332	arg1	clinical					356:363	clinical	356:363	clinical	356:363	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	6	6	theme	mesenchymal	976:986	arg1	BMSCs					1000:1004	BMSCs	1000:1004	BMSCs	1000:1004	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	6	6	theme	mesenchymal	976:986	arg1	cells					993:997	Bone marrow mesenchymal stem cells	964:997	Bone marrow mesenchymal stem cells (BMSCs)	964:1005	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	7	7	theme	materials	1456:1464	arg1	kind					1448:1451	this kind	1443:1451	this kind of materials	1443:1464	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	6	8	theme	fluorescent	1252:1262	arg1	dye					1264:1266	fluorescent dye	1252:1266	fluorescent dye	1252:1266	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	10	9	theme	multifunctional	1915:1929	arg1	coatings					1941:1948	multifunctional composite coatings	1915:1948	multifunctional composite coatings	1915:1948	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	10	9	theme	multifunctional	1915:1929	arg1	osteogenesis					1971:1982	osteogenesis	1971:1982	osteogenesis	1971:1982	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	10	9	theme	multifunctional	1915:1929	arg1	antibiosis					1959:1968	antibiosis	1959:1968	antibiosis	1959:1968	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	10	9	theme	multifunctional	1915:1929	arg1	angiogenesis					1988:1999	angiogenesis	1988:1999	angiogenesis	1988:1999	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	8	10	with	myocardium	1616:1625	arg1	impacts					1659:1665	slightly negative impacts	1641:1665	slightly negative impacts on liver and kidney	1641:1685	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	6	11	theme	marrow	969:974	arg1	BMSCs					1000:1004	BMSCs	1000:1004	BMSCs	1000:1004	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	6	11	theme	marrow	969:974	arg1	cells					993:997	Bone marrow mesenchymal stem cells	964:997	Bone marrow mesenchymal stem cells (BMSCs)	964:1005	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	2	12	theme	Ag	572:573	arg1	Ag-NPs					590:595	Ag-NPs	590:595	Ag-NPs	590:595	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	12	theme	Ag	572:573	arg1	nanoparticles					575:587	coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles	523:587	coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs)	523:596	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	13	theme	Ti	616:617	arg1	surface					605:611	the surface	601:611	the surface of Ti	601:617	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	1	14	used	utilized	171:178	arg2	Ti					127:128	Ti	127:128	Ti	127:128	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	1	14	used	utilized	171:178	arg2	alloy					143:147	titanium alloy	134:147	titanium alloy	134:147	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	7	15	with	biocompatibility	1482:1497	arg1	cells					1509:1513	VECs cells	1504:1513	VECs cells	1504:1513	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	6	16	theme	Bone	964:967	arg1	marrow					969:974	Bone marrow	964:974	Bone marrow mesenchymal stem cells (BMSCs)	964:1005	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	2	17	theme	dual	502:505	arg1	regulations					507:517	dual regulations	502:517	dual regulations	502:517	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	6	18	theme	promising	1148:1156	arg1	capability					1158:1167	promising capability	1148:1167	promising capability	1148:1167	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	3	19	with	nanoparticles	634:646	arg1	distribution					658:669	even distribution	653:669	even distribution	653:669	The spherical nanoparticles with even distribution were fabricated by optimizing deposition potential and the concentration of Ag+.					
31147012	10	20	theme	composite	1931:1939	arg1	coatings					1941:1948	multifunctional composite coatings	1915:1948	multifunctional composite coatings	1915:1948	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	10	20	theme	composite	1931:1939	arg1	osteogenesis					1971:1982	osteogenesis	1971:1982	osteogenesis	1971:1982	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	10	20	theme	composite	1931:1939	arg1	antibiosis					1959:1968	antibiosis	1959:1968	antibiosis	1959:1968	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	10	20	theme	composite	1931:1939	arg1	angiogenesis					1988:1999	angiogenesis	1988:1999	angiogenesis	1988:1999	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	4	21	theme	physical	756:763	arg1	stabilities					765:775	The physical stabilities	752:775	The physical stabilities of coatings	752:787	The physical stabilities of coatings were significantly improved by the chelation among CS, Ag+ and Ca2+ reducing the release rate of Ag+, Ca2+.					
31147012	6	22	dep	time	1111:1114	arg1	revealed					1139:1146	revealed	1139:1146	revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye	1139:1266	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	3	23	theme	deposition	701:710	arg1	potential					712:720	deposition potential	701:720	deposition potential	701:720	The spherical nanoparticles with even distribution were fabricated by optimizing deposition potential and the concentration of Ag+.					
31147012	7	24	theme	Similar	1269:1275	arg1	results					1277:1283	Similar results	1269:1283	Similar results	1269:1283	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	8	25	with	biocompatible	1582:1594	arg1	lung					1631:1634	lung	1631:1634	lung	1631:1634	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	8	25	with	biocompatible	1582:1594	arg1	myocardium					1616:1625	myocardium	1616:1625	myocardium	1616:1625	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	8	25	with	biocompatible	1582:1594	arg1	muscle					1608:1613	smooth muscle	1601:1613	smooth muscle	1601:1613	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	9	26	theme	red	1725:1727	arg1	staining					1729:1736	alizarin red staining	1716:1736	alizarin red staining	1716:1736	According to the results of alizarin red staining, the calcified nodules were dyed red, which reveal that this material can promote bone formation.					
31147012	2	27	theme	electrochemical	390:404	arg1	deposition					406:415	pulse electrochemical deposition	384:415	pulse electrochemical deposition	384:415	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	27	theme	electrochemical	390:404	arg1	method					431:436	a method	429:436	a method	429:436	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	0	28	theme	Osteogenic	0:9	arg1	nanocoating					26:36	Osteogenic and antiseptic nanocoating	0:36	Osteogenic and antiseptic nanocoating by in situ chitosan	0:56	Osteogenic and antiseptic nanocoating by in situ chitosan regulated electrochemical deposition for promoting osseointegration.					
31147012	10	29	used	utilized	1863:1870	arg2	method					1852:1857	Electrochemical method	1836:1857	Electrochemical method	1836:1857	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	6	30	theme	composite	1120:1128	arg1	coatings					1130:1137	the composite coatings	1116:1137	the composite coatings	1116:1137	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	7	31	contain	has	1466:1468	arg2	biocompatibility					1482:1497	outstanding biocompatibility	1470:1497	outstanding biocompatibility with VECs cells	1470:1513	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	7	31	contain	has	1466:1468	arg1	kind					1448:1451	this kind	1443:1451	this kind of materials	1443:1464	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	2	32	theme	pulse	384:388	arg1	deposition					406:415	pulse electrochemical deposition	384:415	pulse electrochemical deposition	384:415	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	32	theme	pulse	384:388	arg1	method					431:436	a method	429:436	a method	429:436	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	3	33	theme	Ag+	747:749	arg1	potential					712:720	deposition potential	701:720	deposition potential	701:720	The spherical nanoparticles with even distribution were fabricated by optimizing deposition potential and the concentration of Ag+.					
31147012	3	33	theme	Ag+	747:749	arg1	concentration					730:742	the concentration	726:742	the concentration of Ag+	726:749	The spherical nanoparticles with even distribution were fabricated by optimizing deposition potential and the concentration of Ag+.					
31147012	2	34	theme	nanoparticles	545:557	arg1	coprecipitation					523:537	coprecipitation	523:537	coprecipitation of HA nanoparticles (HA-NPs)	523:566	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	34	theme	nanoparticles	545:557	arg1	Ag					572:573	Ag	572:573	Ag	572:573	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	8	35	with	lung	1631:1634	arg1	impacts					1659:1665	slightly negative impacts	1641:1665	slightly negative impacts on liver and kidney	1641:1685	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	6	36	theme	proliferation	1027:1039	arg1	abilities					1061:1069	adhesion, proliferation and differentiation abilities	1017:1069	adhesion, proliferation and differentiation abilities	1017:1069	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	0	37	theme	antiseptic	15:24	arg1	nanocoating					26:36	Osteogenic and antiseptic nanocoating	0:36	Osteogenic and antiseptic nanocoating by in situ chitosan	0:56	Osteogenic and antiseptic nanocoating by in situ chitosan regulated electrochemical deposition for promoting osseointegration.					
31147012	8	38	theme	composite	1558:1566	arg1	biocompatible					1582:1594	biocompatible	1582:1594	biocompatible	1582:1594	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	8	38	theme	composite	1558:1566	arg1	coatings					1568:1575	the composite coatings	1554:1575	the composite coatings	1554:1575	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	2	39	theme	HA	542:543	arg1	HA-NPs					560:565	HA-NPs	560:565	HA-NPs	560:565	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	39	theme	HA	542:543	arg1	nanoparticles					545:557	HA nanoparticles	542:557	HA nanoparticles (HA-NPs)	542:566	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	5	40	theme	noticeable	925:934	arg1	abilities					936:944	noticeable abilities	925:944	noticeable abilities	925:944	The coatings also exhibited noticeable abilities in anti-bacteria.					
31147012	8	41	with	muscle	1608:1613	arg1	impacts					1659:1665	slightly negative impacts	1641:1665	slightly negative impacts on liver and kidney	1641:1685	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	1	42	theme	other	212:216	arg1	fields					226:231	other related fields	212:231	other related fields	212:231	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	0	43	theme	in	41:42	arg1	chitosan					49:56	in situ chitosan	41:56	in situ chitosan	41:56	Osteogenic and antiseptic nanocoating by in situ chitosan regulated electrochemical deposition for promoting osseointegration.					
31147012	1	44	from	abilities	251:259	arg1	vascularization					293:307	vascularization	293:307	vascularization	293:307	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	1	44	from	abilities	251:259	arg1	ossification					276:287	ossification	276:287	ossification	276:287	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	1	44	from	abilities	251:259	arg1	antibiosis					264:273	antibiosis	264:273	antibiosis	264:273	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	6	45	theme	adhesion	1017:1024	arg1	abilities					1061:1069	adhesion, proliferation and differentiation abilities	1017:1069	adhesion, proliferation and differentiation abilities	1017:1069	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	1	46	theme	orthopedics	196:206	arg1	areas					187:191	the areas	183:191	the areas of orthopedics and other related fields	183:231	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	1	47	theme	related	218:224	arg1	fields					226:231	other related fields	212:231	other related fields	212:231	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	6	48	theme	differentiation	1045:1059	arg1	abilities					1061:1069	adhesion, proliferation and differentiation abilities	1017:1069	adhesion, proliferation and differentiation abilities	1017:1069	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	2	49	theme	coprecipitation	523:537	arg1	Ag-NPs					590:595	Ag-NPs	590:595	Ag-NPs	590:595	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	49	theme	coprecipitation	523:537	arg1	nanoparticles					575:587	coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles	523:587	coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs)	523:596	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	10	50	theme	Electrochemical	1836:1850	arg1	method					1852:1857	Electrochemical method	1836:1857	Electrochemical method	1836:1857	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	2	51	used	used	421:424	arg2	method					431:436	a method	429:436	a method	429:436	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	51	used	used	421:424	arg2	deposition					406:415	pulse electrochemical deposition	384:415	pulse electrochemical deposition	384:415	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	1	52	theme	fields	226:231	arg1	areas					187:191	the areas	183:191	the areas of orthopedics and other related fields	183:231	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	9	53	theme	bone	1820:1823	arg1	formation					1825:1833	bone formation	1820:1833	bone formation	1820:1833	According to the results of alizarin red staining, the calcified nodules were dyed red, which reveal that this material can promote bone formation.					
31147012	8	54	theme	smooth	1601:1606	arg1	muscle					1608:1613	smooth muscle	1601:1613	smooth muscle	1601:1613	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	9	55	theme	alizarin	1716:1723	arg1	staining					1729:1736	alizarin red staining	1716:1736	alizarin red staining	1716:1736	According to the results of alizarin red staining, the calcified nodules were dyed red, which reveal that this material can promote bone formation.					
31147012	0	56	dep	in	41:42	arg1	situ					44:47	situ	44:47	situ	44:47	Osteogenic and antiseptic nanocoating by in situ chitosan regulated electrochemical deposition for promoting osseointegration.					
31147012	4	57	theme	Ag+	886:888	arg1	Ca2+					891:894	Ag+, Ca2+	886:894	Ca2+	891:894	The physical stabilities of coatings were significantly improved by the chelation among CS, Ag+ and Ca2+ reducing the release rate of Ag+, Ca2+.					
31147012	6	58	theme	same	1106:1109	arg1	time					1111:1114	the same time	1102:1114	the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye	1102:1266	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	8	59	from	impacts	1659:1665	arg1	kidney					1680:1685	kidney	1680:1685	kidney	1680:1685	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	8	59	from	impacts	1659:1665	arg1	liver					1670:1674	liver	1670:1674	liver	1670:1674	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	4	60	theme	release	870:876	arg1	rate					878:881	the release rate	866:881	the release rate of Ag+, Ca2+	866:894	The physical stabilities of coatings were significantly improved by the chelation among CS, Ag+ and Ca2+ reducing the release rate of Ag+, Ca2+.					
31147012	7	61	theme	VECs	1504:1507	arg1	cells					1509:1513	VECs cells	1504:1513	VECs cells	1504:1513	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	7	62	theme	desirable	1355:1363	arg1	adhesion					1365:1372	desirable adhesion	1355:1372	desirable adhesion between cells and materials	1355:1400	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	1	63	theme	titanium	134:141	arg1	alloy					143:147	titanium alloy	134:147	titanium alloy	134:147	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	2	64	dep	ions	496:499	arg1	Ag-NPs					590:595	Ag-NPs	590:595	Ag-NPs	590:595	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	64	dep	ions	496:499	arg1	regulations					507:517	dual regulations	502:517	dual regulations	502:517	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	2	64	dep	ions	496:499	arg1	nanoparticles					575:587	coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles	523:587	coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs)	523:596	In this research, pulse electrochemical deposition was used as a method to make chitosan regulate Ag+ and Ca2+ in situ, achieving ions' dual regulations and coprecipitation of HA nanoparticles (HA-NPs) and Ag nanoparticles (Ag-NPs) on the surface of Ti.					
31147012	3	65	theme	even	653:656	arg1	distribution					658:669	even distribution	653:669	even distribution	653:669	The spherical nanoparticles with even distribution were fabricated by optimizing deposition potential and the concentration of Ag+.					
31147012	4	66	theme	coatings	780:787	arg1	stabilities					765:775	The physical stabilities	752:775	The physical stabilities of coatings	752:787	The physical stabilities of coatings were significantly improved by the chelation among CS, Ag+ and Ca2+ reducing the release rate of Ag+, Ca2+.					
31147012	8	67	theme	negative	1650:1657	arg1	impacts					1659:1665	slightly negative impacts	1641:1665	slightly negative impacts on liver and kidney	1641:1685	The animal experiments indicated that the composite coatings were biocompatible with smooth muscle, myocardium and lung with slightly negative impacts on liver and kidney.					
31147012	10	68	theme	Ti	2020:2021	arg1	surface					2009:2015	the surface	2005:2015	the surface of Ti	2005:2021	Electrochemical method was utilized in this research to successfully construct multifunctional composite coatings, such as antibiosis, osteogenesis and angiogenesis, on the surface of Ti.					
31147012	3	69	theme	spherical	624:632	arg1	nanoparticles					634:646	The spherical nanoparticles	620:646	The spherical nanoparticles with even distribution	620:669	The spherical nanoparticles with even distribution were fabricated by optimizing deposition potential and the concentration of Ag+.					
31147012	7	70	theme	endothelial	1336:1346	arg1	cells					1348:1352	vascular endothelial cells	1327:1352	vascular endothelial cells	1327:1352	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	7	71	located	found	1297:1301	arg2	results					1277:1283	Similar results	1269:1283	Similar results	1269:1283	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	7	71	located	found	1297:1301	arg1	investigations					1306:1319	investigations	1306:1319	investigations about vascular endothelial cells	1306:1352	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	0	72	theme	electrochemical	68:82	arg1	deposition					84:93	electrochemical deposition	68:93	electrochemical deposition for promoting osseointegration	68:124	Osteogenic and antiseptic nanocoating by in situ chitosan regulated electrochemical deposition for promoting osseointegration.					
31147012	4	73	theme	Ca2+	891:894	arg1	rate					878:881	the release rate	866:881	the release rate of Ag+, Ca2+	866:894	The physical stabilities of coatings were significantly improved by the chelation among CS, Ag+ and Ca2+ reducing the release rate of Ag+, Ca2+.					
31147012	6	74	theme	BMSCs	1181:1185	arg1	differentiation					1187:1201	BMSCs differentiation	1181:1201	BMSCs differentiation	1181:1201	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	6	75	theme	coatings	1089:1096	arg1	surface					1078:1084	the surface	1074:1084	the surface of coatings	1074:1096	Bone marrow mesenchymal stem cells (BMSCs) displayed adhesion, proliferation and differentiation abilities on the surface of coatings, at the same time the composite coatings revealed promising capability in inducing BMSCs differentiation to osteoblast, which is proved by the results of fluorescent dye.					
31147012	9	76	theme	calcified	1743:1751	arg1	nodules					1753:1759	the calcified nodules	1739:1759	the calcified nodules	1739:1759	According to the results of alizarin red staining, the calcified nodules were dyed red, which reveal that this material can promote bone formation.					
31147012	9	76	theme	calcified	1743:1751	arg1	red					1771:1773	red	1771:1773	red	1771:1773	According to the results of alizarin red staining, the calcified nodules were dyed red, which reveal that this material can promote bone formation.					
31147012	1	77	theme	materials	343:351	arg1	application					322:332	the application	318:332	the application of these materials in clinical	318:363	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31147012	7	78	theme	vascular	1327:1334	arg1	cells					1348:1352	vascular endothelial cells	1327:1352	vascular endothelial cells	1327:1352	Similar results also can be found in investigations about vascular endothelial cells, desirable adhesion between cells and materials and proliferation are able to prove that this kind of materials has outstanding biocompatibility with VECs cells.					
31147012	1	79	theme	limited	243:249	arg1	abilities					251:259	limited abilities	243:259	limited abilities in antibiosis, ossification and vascularization	243:307	Ti and titanium alloy have been extensively utilized in the areas of orthopedics and other related fields, however, limited abilities in antibiosis, ossification and vascularization restrict the application of these materials in clinical.					
31580960	14	0	theme	longitudinal	2407:2418	arg1	quantification					2440:2453	longitudinal and non-destructive quantification	2407:2453	longitudinal and non-destructive quantification of GAGs	2407:2461	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	6	1	theme	high	1242:1245	arg1	correlation					1254:1264	a high linear correlation	1240:1264	a high linear correlation	1240:1264	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	14	2	theme	valuable	2474:2481	arg1	insight					2483:2489	valuable insight	2474:2489	valuable insight on GAG development and distribution in cartilage engineered constructs	2474:2560	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	10	3	from	imaging	1705:1711	arg1	constructs					1764:1773	cartilage tissue-engineered constructs	1736:1773	cartilage tissue-engineered constructs	1736:1773	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	10	4	theme	charged	1647:1653	arg1	agent					1664:1668	a positively charged contrast agent	1634:1668	a positively charged contrast agent	1634:1668	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	4	5	theme	minimal	927:933	arg1	dose					945:948	minimal radiation dose	927:948	minimal radiation dose	927:948	CA4+ concentration and scanning protocols were first optimized to ensure no cytotoxicity and a facile procedure with minimal radiation dose.					
31580960	9	6	theme	histological	1540:1551	arg1	sections					1553:1560	histological sections	1540:1560	histological sections	1540:1560	Additionally, the CA4+ distribution accurately matched safranin-O staining on histological sections.					
31580960	6	7	theme	blue	1214:1217	arg1	analysis					1219:1226	1,9-dimethylmethylene blue analysis	1192:1226	1,9-dimethylmethylene blue analysis	1192:1226	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	8	8	theme	cell	1428:1431	arg1	viability					1433:1441	cell viability	1428:1441	cell viability	1428:1441	Importantly, this method did not adversely affect cell viability or chondrogenesis.					
31580960	11	9	theme	cartilage	1925:1933	arg1	defects					1935:1941	cartilage defects	1925:1941	cartilage defects	1925:1941	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	3	10	theme	contrast-enhanced	607:623	arg1	tomography					634:643	contrast-enhanced computed tomography	607:643	contrast-enhanced computed tomography	607:643	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	10	11	theme	GAG	1716:1718	arg1	distribution					1720:1731	GAG distribution	1716:1731	GAG distribution	1716:1731	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	3	12	theme	-based	651:656	arg1	method					658:663	a contrast-enhanced computed tomography (CECT)-based method	605:663	a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs	605:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	15	13	theme	pre-implantation	2702:2717	arg1	tool					2729:2732	a pre-implantation screening tool	2700:2732	a pre-implantation screening tool	2700:2732	Such technique can advance the development of regenerative strategies, not only by allowing continuous monitoring but also by serving as a pre-implantation screening tool.					
31580960	0	14	from	glycosaminoglycans	70:87	arg1	constructs					120:129	cartilage tissue engineered constructs	92:129	cartilage tissue engineered constructs	92:129	Contrast enhanced computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs.					
31580960	5	15	contain	containing	998:1007	arg2	content					1023:1029	different GAG content	1009:1029	different GAG content	1009:1029	Chondrocyte and mesenchymal stem cell pellets, containing different GAG content were generated and exposed to CA4+.					
31580960	5	15	contain	containing	998:1007	arg1	pellets					989:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets, containing different GAG content were generated and exposed to CA4+.					
31580960	15	16	theme	Such	2563:2566	arg1	technique					2568:2576	Such technique	2563:2576	Such technique	2563:2576	Such technique can advance the development of regenerative strategies, not only by allowing continuous monitoring but also by serving as a pre-implantation screening tool.					
31580960	3	17	theme	cationic	673:680	arg1	agent					701:705	a cationic iodinated contrast agent	671:705	a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs	671:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	3	17	theme	cationic	673:680	arg1	CA4+					708:711	CA4+	708:711	CA4+	708:711	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	5	18	theme	different	1009:1017	arg1	content					1023:1029	different GAG content	1009:1029	different GAG content	1009:1029	Chondrocyte and mesenchymal stem cell pellets, containing different GAG content were generated and exposed to CA4+.					
31580960	14	19	theme	positively-charged	2369:2386	arg1	agent					2397:2401	a positively-charged contrast agent	2367:2401	a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs	2367:2461	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	2	20	theme	matrix	558:563	arg1	components					565:574	cartilage matrix components	548:574	cartilage matrix components	548:574	Insights into the processes occurring during regeneration are essential to steer and inform development of the envisaged regenerative strategy, however tools are needed for longitudinal and quantitative monitoring of cartilage matrix components.					
31580960	7	21	theme	culture	1369:1375	arg1	28 days					1351:1357	28 days	1351:1357	28 days of pellet culture	1351:1375	The established equation was used for longitudinal measurements of GAG content over 28 days of pellet culture.					
31580960	14	22	theme	cartilage	2530:2538	arg1	constructs					2551:2560	cartilage engineered constructs	2530:2560	cartilage engineered constructs	2530:2560	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	4	23	with	cytotoxicity	886:897	arg1	dose					945:948	minimal radiation dose	927:948	minimal radiation dose	927:948	CA4+ concentration and scanning protocols were first optimized to ensure no cytotoxicity and a facile procedure with minimal radiation dose.					
31580960	0	24	theme	glycosaminoglycans	70:87	arg1	quantification					52:65	real-time quantification	42:65	real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs	42:129	Contrast enhanced computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs.					
31580960	15	25	theme	continuous	2655:2664	arg1	monitoring					2666:2675	continuous monitoring	2655:2675	continuous monitoring	2655:2675	Such technique can advance the development of regenerative strategies, not only by allowing continuous monitoring but also by serving as a pre-implantation screening tool.					
31580960	5	26	theme	mesenchymal	967:977	arg1	pellets					989:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets, containing different GAG content were generated and exposed to CA4+.					
31580960	0	27	theme	cartilage	92:100	arg1	tissue					102:107	cartilage tissue	92:107	cartilage tissue engineered constructs	92:129	Contrast enhanced computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs.					
31580960	13	28	theme	cartilage-engineered	2222:2241	arg1	constructs					2243:2252	cartilage-engineered constructs	2222:2252	cartilage-engineered constructs	2222:2252	Therefore, there is a need for the development of techniques that allow for longitudinal and quantitative imaging and monitoring of cartilage-engineered constructs.					
31580960	5	29	theme	cell	984:987	arg1	pellets					989:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets, containing different GAG content were generated and exposed to CA4+.					
31580960	4	30	with	procedure	912:920	arg1	dose					945:948	minimal radiation dose	927:948	minimal radiation dose	927:948	CA4+ concentration and scanning protocols were first optimized to ensure no cytotoxicity and a facile procedure with minimal radiation dose.					
31580960	6	31	theme	CA4+	1071:1074	arg1	content					1076:1082	The CA4+ content	1067:1082	The CA4+ content	1067:1082	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	14	32	theme	electrostatic	2279:2291	arg1	interactions					2293:2304	the electrostatic interactions	2275:2304	the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs	2275:2461	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	4	33	theme	scanning	833:840	arg1	protocols					842:850	scanning protocols	833:850	scanning protocols	833:850	CA4+ concentration and scanning protocols were first optimized to ensure no cytotoxicity and a facile procedure with minimal radiation dose.					
31580960	11	34	theme	joint	1899:1903	arg1	pathologies					1905:1915	different joint pathologies	1889:1915	different joint pathologies such as cartilage defects or osteoarthritis	1889:1959	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	11	34	theme	joint	1899:1903	arg1	osteoarthritis					1946:1959	osteoarthritis	1946:1959	osteoarthritis	1946:1959	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	11	34	theme	joint	1899:1903	arg1	defects					1935:1941	cartilage defects	1925:1941	cartilage defects	1925:1941	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	0	35	theme	engineered	109:118	arg1	constructs					120:129	cartilage tissue engineered constructs	92:129	cartilage tissue engineered constructs	92:129	Contrast enhanced computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs.					
31580960	12	36	theme	destructive	2069:2079	arg1	methods					2081:2087	destructive methods	2069:2087	destructive methods	2069:2087	Currently, in vitro assessment on the quality and composition of the engineered cartilage mainly relies on destructive methods.					
31580960	15	37	dep	advance	2582:2588	arg1	allowing					2646:2653	allowing	2646:2653	allowing continuous monitoring	2646:2675	Such technique can advance the development of regenerative strategies, not only by allowing continuous monitoring but also by serving as a pre-implantation screening tool.					
31580960	15	37	dep	advance	2582:2588	arg1	serving					2689:2695	serving	2689:2695	serving as a pre-implantation screening tool	2689:2732	Such technique can advance the development of regenerative strategies, not only by allowing continuous monitoring but also by serving as a pre-implantation screening tool.					
31580960	0	38	from	quantification	52:65	arg1	constructs					120:129	cartilage tissue engineered constructs	92:129	cartilage tissue engineered constructs	92:129	Contrast enhanced computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs.					
31580960	3	39	from	constructs	798:807	arg1	quantification					731:744	longitudinal quantification	718:744	longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs	718:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	15	40	theme	strategies	2622:2631	arg1	development					2594:2604	the development	2590:2604	the development of regenerative strategies	2590:2631	Such technique can advance the development of regenerative strategies, not only by allowing continuous monitoring but also by serving as a pre-implantation screening tool.					
31580960	14	41	theme	contrast	2388:2395	arg1	agent					2397:2401	a positively-charged contrast agent	2367:2401	a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs	2367:2461	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	6	42	from	content	1076:1082	arg1	pellets					1091:1097	the pellets	1087:1097	the pellets	1087:1097	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	11	43	theme	SIGNIFICANCE	1789:1800	arg1	STATEMENT					1776:1784	STATEMENT	1776:1784	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine	1776:1846	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	11	43	theme	SIGNIFICANCE	1789:1800	arg1	strategies					1874:1883	promising therapeutic strategies	1852:1883	promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis	1852:1959	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	10	44	theme	cartilage	1736:1744	arg1	constructs					1764:1773	cartilage tissue-engineered constructs	1736:1773	cartilage tissue-engineered constructs	1736:1773	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	12	45	theme	cartilage	2042:2050	arg1	composition					2012:2022	composition	2012:2022	composition	2012:2022	Currently, in vitro assessment on the quality and composition of the engineered cartilage mainly relies on destructive methods.					
31580960	12	45	theme	cartilage	2042:2050	arg1	quality					2000:2006	quality	2000:2006	quality	2000:2006	Currently, in vitro assessment on the quality and composition of the engineered cartilage mainly relies on destructive methods.					
31580960	6	46	theme	computed	1123:1130	arg1	tomography					1132:1141	micro computed tomography	1117:1141	micro computed tomography	1117:1141	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	0	47	theme	computed	18:25	arg1	tomography					27:36	computed tomography	18:36	computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs	18:129	Contrast enhanced computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs.					
31580960	1	48	theme	Tissue	132:137	arg1	engineering					139:149	Tissue engineering	132:149	Tissue engineering	132:149	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	1	48	theme	Tissue	132:137	arg1	medicine					168:175	regenerative medicine	155:175	regenerative medicine	155:175	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	1	48	theme	Tissue	132:137	arg1	strategies					197:206	two therapeutic strategies	181:206	two therapeutic strategies	181:206	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	2	49	theme	quantitative	521:532	arg1	monitoring					534:543	longitudinal and quantitative monitoring	504:543	longitudinal and quantitative monitoring of cartilage matrix components	504:574	Insights into the processes occurring during regeneration are essential to steer and inform development of the envisaged regenerative strategy, however tools are needed for longitudinal and quantitative monitoring of cartilage matrix components.					
31580960	2	50	theme	regenerative	452:463	arg1	strategy					465:472	the envisaged regenerative strategy	438:472	the envisaged regenerative strategy	438:472	Insights into the processes occurring during regeneration are essential to steer and inform development of the envisaged regenerative strategy, however tools are needed for longitudinal and quantitative monitoring of cartilage matrix components.					
31580960	11	51	theme	therapeutic	1862:1872	arg1	STATEMENT					1776:1784	STATEMENT	1776:1784	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine	1776:1846	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	11	51	theme	therapeutic	1862:1872	arg1	strategies					1874:1883	promising therapeutic strategies	1852:1883	promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis	1852:1959	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	7	52	theme	pellet	1362:1367	arg1	culture					1369:1375	pellet culture	1362:1375	pellet culture	1362:1375	The established equation was used for longitudinal measurements of GAG content over 28 days of pellet culture.					
31580960	2	53	theme	longitudinal	504:515	arg1	monitoring					534:543	longitudinal and quantitative monitoring	504:543	longitudinal and quantitative monitoring of cartilage matrix components	504:574	Insights into the processes occurring during regeneration are essential to steer and inform development of the envisaged regenerative strategy, however tools are needed for longitudinal and quantitative monitoring of cartilage matrix components.					
31580960	10	54	theme	CECT	1618:1621	arg1	application					1603:1613	the application	1599:1613	the application of CECT	1599:1621	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	3	55	theme	contrast	692:699	arg1	agent					701:705	a cationic iodinated contrast agent	671:705	a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs	671:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	3	55	theme	contrast	692:699	arg1	CA4+					708:711	CA4+	708:711	CA4+	708:711	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	12	56	theme	in	1973:1974	arg1	assessment					1982:1991	in vitro assessment	1973:1991	in vitro assessment on the quality and composition of the engineered cartilage	1973:2050	Currently, in vitro assessment on the quality and composition of the engineered cartilage mainly relies on destructive methods.					
31580960	12	57	dep	quality	2000:2006	arg1	the					1996:1998	the	1996:1998	the	1996:1998	Currently, in vitro assessment on the quality and composition of the engineered cartilage mainly relies on destructive methods.					
31580960	11	58	theme	promising	1852:1860	arg1	STATEMENT					1776:1784	STATEMENT	1776:1784	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine	1776:1846	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	11	58	theme	promising	1852:1860	arg1	strategies					1874:1883	promising therapeutic strategies	1852:1883	promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis	1852:1959	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	13	59	theme	longitudinal	2166:2177	arg1	imaging					2196:2202	longitudinal and quantitative imaging	2166:2202	longitudinal and quantitative imaging	2166:2202	Therefore, there is a need for the development of techniques that allow for longitudinal and quantitative imaging and monitoring of cartilage-engineered constructs.					
31580960	7	60	theme	content	1338:1344	arg1	measurements					1318:1329	longitudinal measurements	1305:1329	longitudinal measurements of GAG content over 28 days of pellet culture	1305:1375	The established equation was used for longitudinal measurements of GAG content over 28 days of pellet culture.					
31580960	1	61	theme	cartilaginous	262:274	arg1	tissues					276:282	cartilaginous tissues	262:282	cartilaginous tissues	262:282	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	6	62	theme	GAG	1164:1166	arg1	content					1168:1174	GAG content	1164:1174	GAG content	1164:1174	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	11	63	theme	Tissue	1803:1808	arg1	engineering					1810:1820	Tissue engineering	1803:1820	Tissue engineering	1803:1820	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	7	64	theme	established	1271:1281	arg1	equation					1283:1290	The established equation	1267:1290	The established equation	1267:1290	The established equation was used for longitudinal measurements of GAG content over 28 days of pellet culture.					
31580960	13	65	theme	techniques	2140:2149	arg1	development					2125:2135	the development	2121:2135	the development of techniques that allow for longitudinal and quantitative imaging and monitoring of cartilage-engineered constructs	2121:2252	Therefore, there is a need for the development of techniques that allow for longitudinal and quantitative imaging and monitoring of cartilage-engineered constructs.					
31580960	9	66	theme	CA4+	1480:1483	arg1	distribution					1485:1496	the CA4+ distribution	1476:1496	the CA4+ distribution	1476:1496	Additionally, the CA4+ distribution accurately matched safranin-O staining on histological sections.					
31580960	4	67	theme	radiation	935:943	arg1	dose					945:948	minimal radiation dose	927:948	minimal radiation dose	927:948	CA4+ concentration and scanning protocols were first optimized to ensure no cytotoxicity and a facile procedure with minimal radiation dose.					
31580960	7	68	theme	longitudinal	1305:1316	arg1	measurements					1318:1329	longitudinal measurements	1305:1329	longitudinal measurements of GAG content over 28 days of pellet culture	1305:1375	The established equation was used for longitudinal measurements of GAG content over 28 days of pellet culture.					
31580960	5	69	theme	stem	979:982	arg1	pellets					989:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets, containing different GAG content were generated and exposed to CA4+.					
31580960	10	70	theme	contrast	1655:1662	arg1	agent					1664:1668	a positively charged contrast agent	1634:1668	a positively charged contrast agent	1634:1668	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	3	71	theme	cartilage-engineered	777:796	arg1	constructs					798:807	cartilage-engineered constructs	777:807	cartilage-engineered constructs	777:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	9	72	theme	safranin-O	1517:1526	arg1	staining					1528:1535	safranin-O staining	1517:1535	safranin-O staining	1517:1535	Additionally, the CA4+ distribution accurately matched safranin-O staining on histological sections.					
31580960	4	73	theme	facile	905:910	arg1	procedure					912:920	a facile procedure	903:920	a facile procedure with minimal radiation dose	903:948	CA4+ concentration and scanning protocols were first optimized to ensure no cytotoxicity and a facile procedure with minimal radiation dose.					
31580960	10	74	theme	quantitative	1692:1703	arg1	imaging					1705:1711	longitudinal and quantitative imaging	1675:1711	longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs	1675:1773	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	3	75	theme	computed	625:632	arg1	tomography					634:643	contrast-enhanced computed tomography	607:643	contrast-enhanced computed tomography	607:643	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	10	76	theme	longitudinal	1675:1686	arg1	imaging					1705:1711	longitudinal and quantitative imaging	1675:1711	longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs	1675:1773	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	6	77	theme	1,9-dimethylmethylene	1192:1212	arg1	analysis					1219:1226	1,9-dimethylmethylene blue analysis	1192:1226	1,9-dimethylmethylene blue analysis	1192:1226	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	3	78	from	quantification	731:744	arg1	constructs					798:807	cartilage-engineered constructs	777:807	cartilage-engineered constructs	777:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	15	79	theme	regenerative	2609:2620	arg1	strategies					2622:2631	regenerative strategies	2609:2631	regenerative strategies	2609:2631	Such technique can advance the development of regenerative strategies, not only by allowing continuous monitoring but also by serving as a pre-implantation screening tool.					
31580960	3	80	dep	-based	651:656	arg1	tomography					634:643	contrast-enhanced computed tomography	607:643	contrast-enhanced computed tomography	607:643	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	3	81	from	glycosaminoglycans	749:766	arg1	constructs					798:807	cartilage-engineered constructs	777:807	cartilage-engineered constructs	777:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	15	82	theme	screening	2719:2727	arg1	tool					2729:2732	a pre-implantation screening tool	2700:2732	a pre-implantation screening tool	2700:2732	Such technique can advance the development of regenerative strategies, not only by allowing continuous monitoring but also by serving as a pre-implantation screening tool.					
31580960	12	83	from	assessment	1982:1991	arg1	composition					2012:2022	composition	2012:2022	composition	2012:2022	Currently, in vitro assessment on the quality and composition of the engineered cartilage mainly relies on destructive methods.					
31580960	12	83	from	assessment	1982:1991	arg1	quality					2000:2006	quality	2000:2006	quality	2000:2006	Currently, in vitro assessment on the quality and composition of the engineered cartilage mainly relies on destructive methods.					
31580960	3	84	theme	longitudinal	718:729	arg1	quantification					731:744	longitudinal quantification	718:744	longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs	718:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	1	85	theme	regenerative	155:166	arg1	engineering					139:149	Tissue engineering	132:149	Tissue engineering	132:149	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	1	85	theme	regenerative	155:166	arg1	medicine					168:175	regenerative medicine	155:175	regenerative medicine	155:175	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	1	85	theme	regenerative	155:166	arg1	strategies					197:206	two therapeutic strategies	181:206	two therapeutic strategies	181:206	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	5	86	theme	Chondrocyte	951:961	arg1	pellets					989:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets	951:995	Chondrocyte and mesenchymal stem cell pellets, containing different GAG content were generated and exposed to CA4+.					
31580960	0	87	from	constructs	120:129	arg1	quantification					52:65	real-time quantification	42:65	real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs	42:129	Contrast enhanced computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs.					
31580960	14	88	theme	engineered	2540:2549	arg1	constructs					2551:2560	cartilage engineered constructs	2530:2560	cartilage engineered constructs	2530:2560	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	11	89	theme	different	1889:1897	arg1	pathologies					1905:1915	different joint pathologies	1889:1915	different joint pathologies such as cartilage defects or osteoarthritis	1889:1959	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	11	89	theme	different	1889:1897	arg1	osteoarthritis					1946:1959	osteoarthritis	1946:1959	osteoarthritis	1946:1959	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	11	89	theme	different	1889:1897	arg1	defects					1935:1941	cartilage defects	1925:1941	cartilage defects	1925:1941	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	14	90	from	distribution	2514:2525	arg1	constructs					2551:2560	cartilage engineered constructs	2530:2560	cartilage engineered constructs	2530:2560	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	2	91	theme	cartilage	548:556	arg1	components					565:574	cartilage matrix components	548:574	cartilage matrix components	548:574	Insights into the processes occurring during regeneration are essential to steer and inform development of the envisaged regenerative strategy, however tools are needed for longitudinal and quantitative monitoring of cartilage matrix components.					
31580960	6	92	theme	linear	1247:1252	arg1	correlation					1254:1264	a high linear correlation	1240:1264	a high linear correlation	1240:1264	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	0	93	theme	tissue	102:107	arg1	constructs					120:129	cartilage tissue engineered constructs	92:129	cartilage tissue engineered constructs	92:129	Contrast enhanced computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs.					
31580960	1	94	theme	therapeutic	185:195	arg1	engineering					139:149	Tissue engineering	132:149	Tissue engineering	132:149	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	1	94	theme	therapeutic	185:195	arg1	medicine					168:175	regenerative medicine	155:175	regenerative medicine	155:175	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	1	94	theme	therapeutic	185:195	arg1	strategies					197:206	two therapeutic strategies	181:206	two therapeutic strategies	181:206	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	6	95	dep	content	1076:1082	arg1	determined					1103:1112	determined	1103:1112	determined by micro computed tomography	1103:1141	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	13	96	theme	constructs	2243:2252	arg1	imaging					2196:2202	longitudinal and quantitative imaging	2166:2202	longitudinal and quantitative imaging	2166:2202	Therefore, there is a need for the development of techniques that allow for longitudinal and quantitative imaging and monitoring of cartilage-engineered constructs.					
31580960	13	96	theme	constructs	2243:2252	arg1	monitoring					2208:2217	monitoring	2208:2217	monitoring of cartilage-engineered constructs	2208:2252	Therefore, there is a need for the development of techniques that allow for longitudinal and quantitative imaging and monitoring of cartilage-engineered constructs.					
31580960	7	97	used	used	1296:1299	arg2	equation					1283:1290	The established equation	1267:1290	The established equation	1267:1290	The established equation was used for longitudinal measurements of GAG content over 28 days of pellet culture.					
31580960	14	98	theme	GAG	2494:2496	arg1	development					2498:2508	GAG development	2494:2508	GAG development	2494:2508	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	13	99	theme	quantitative	2183:2194	arg1	imaging					2196:2202	longitudinal and quantitative imaging	2166:2202	longitudinal and quantitative imaging	2166:2202	Therefore, there is a need for the development of techniques that allow for longitudinal and quantitative imaging and monitoring of cartilage-engineered constructs.					
31580960	12	100	theme	engineered	2031:2040	arg1	cartilage					2042:2050	the engineered cartilage	2027:2050	the engineered cartilage	2027:2050	Currently, in vitro assessment on the quality and composition of the engineered cartilage mainly relies on destructive methods.					
31580960	14	101	from	insight	2483:2489	arg1	distribution					2514:2525	distribution	2514:2525	distribution	2514:2525	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	14	101	from	insight	2483:2489	arg1	development					2498:2508	GAG development	2494:2508	GAG development	2494:2508	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	2	102	theme	components	565:574	arg1	monitoring					534:543	longitudinal and quantitative monitoring	504:543	longitudinal and quantitative monitoring of cartilage matrix components	504:574	Insights into the processes occurring during regeneration are essential to steer and inform development of the envisaged regenerative strategy, however tools are needed for longitudinal and quantitative monitoring of cartilage matrix components.					
31580960	10	103	theme	distribution	1720:1731	arg1	imaging					1705:1711	longitudinal and quantitative imaging	1675:1711	longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs	1675:1773	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	10	104	theme	tissue-engineered	1746:1762	arg1	constructs					1764:1773	cartilage tissue-engineered constructs	1736:1773	cartilage tissue-engineered constructs	1736:1773	Hence, we show proof-of-concept for the application of CECT, utilizing a positively charged contrast agent, for longitudinal and quantitative imaging of GAG distribution in cartilage tissue-engineered constructs.					
31580960	5	105	theme	GAG	1019:1021	arg1	content					1023:1029	different GAG content	1009:1029	different GAG content	1009:1029	Chondrocyte and mesenchymal stem cell pellets, containing different GAG content were generated and exposed to CA4+.					
31580960	1	106	dep	strategies	197:206	arg1	cure					237:240	cure	237:240	cure	237:240	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	1	106	dep	strategies	197:206	arg1	treat					211:215	treat	211:215	to treat	208:215	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	12	107	dep	in	1973:1974	arg1	vitro					1976:1980	vitro	1976:1980	vitro	1976:1980	Currently, in vitro assessment on the quality and composition of the engineered cartilage mainly relies on destructive methods.					
31580960	1	108	theme	cartilage	312:320	arg1	defects					322:328	cartilage defects	312:328	cartilage defects	312:328	Tissue engineering and regenerative medicine are two therapeutic strategies to treat, and to potentially cure, diseases affecting cartilaginous tissues, such as osteoarthritis and cartilage defects.					
31580960	6	109	theme	micro	1117:1121	arg1	tomography					1132:1141	micro computed tomography	1117:1141	micro computed tomography	1117:1141	The CA4+ content in the pellets, as determined by micro computed tomography, was plotted against GAG content, as measured by 1,9-dimethylmethylene blue analysis, and showed a high linear correlation.					
31580960	4	110	theme	CA4+	810:813	arg1	concentration					815:827	CA4+ concentration	810:827	CA4+ concentration	810:827	CA4+ concentration and scanning protocols were first optimized to ensure no cytotoxicity and a facile procedure with minimal radiation dose.					
31580960	14	111	theme	GAGs	2458:2461	arg1	quantification					2440:2453	longitudinal and non-destructive quantification	2407:2453	longitudinal and non-destructive quantification of GAGs	2407:2461	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	2	112	theme	strategy	465:472	arg1	development					423:433	development	423:433	development of the envisaged regenerative strategy	423:472	Insights into the processes occurring during regeneration are essential to steer and inform development of the envisaged regenerative strategy, however tools are needed for longitudinal and quantitative monitoring of cartilage matrix components.					
31580960	0	113	theme	real-time	42:50	arg1	quantification					52:65	real-time quantification	42:65	real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs	42:129	Contrast enhanced computed tomography for real-time quantification of glycosaminoglycans in cartilage tissue engineered constructs.					
31580960	3	114	theme	iodinated	682:690	arg1	agent					701:705	a cationic iodinated contrast agent	671:705	a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs	671:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	3	114	theme	iodinated	682:690	arg1	CA4+					708:711	CA4+	708:711	CA4+	708:711	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31580960	2	115	theme	envisaged	442:450	arg1	strategy					465:472	the envisaged regenerative strategy	438:472	the envisaged regenerative strategy	438:472	Insights into the processes occurring during regeneration are essential to steer and inform development of the envisaged regenerative strategy, however tools are needed for longitudinal and quantitative monitoring of cartilage matrix components.					
31580960	11	116	dep	STATEMENT	1776:1784	arg1	engineering					1810:1820	Tissue engineering	1803:1820	Tissue engineering	1803:1820	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	11	116	dep	STATEMENT	1776:1784	arg1	medicine					1839:1846	regenerative medicine	1826:1846	regenerative medicine	1826:1846	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	14	117	theme	non-destructive	2424:2438	arg1	quantification					2440:2453	longitudinal and non-destructive quantification	2407:2453	longitudinal and non-destructive quantification of GAGs	2407:2461	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	7	118	theme	GAG	1334:1336	arg1	content					1338:1344	GAG content	1334:1344	GAG content over 28 days of pellet culture	1334:1375	The established equation was used for longitudinal measurements of GAG content over 28 days of pellet culture.					
31580960	11	119	theme	regenerative	1826:1837	arg1	medicine					1839:1846	regenerative medicine	1826:1846	regenerative medicine	1826:1846	STATEMENT OF SIGNIFICANCE: Tissue engineering and regenerative medicine are promising therapeutic strategies for different joint pathologies such as cartilage defects or osteoarthritis.					
31580960	14	120	from	development	2498:2508	arg1	constructs					2551:2560	cartilage engineered constructs	2530:2560	cartilage engineered constructs	2530:2560	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	14	121	theme	negatively-charged	2318:2335	arg1	glycosaminoglycans					2337:2354	the negatively-charged glycosaminoglycans	2314:2354	the negatively-charged glycosaminoglycans (GAGs)	2314:2361	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	14	121	theme	negatively-charged	2318:2335	arg1	GAGs					2357:2360	GAGs	2357:2360	GAGs	2357:2360	This work harnesses the electrostatic interactions between the negatively-charged glycosaminoglycans (GAGs) and a positively-charged contrast agent for longitudinal and non-destructive quantification of GAGs, providing valuable insight on GAG development and distribution in cartilage engineered constructs.					
31580960	3	122	theme	glycosaminoglycans	749:766	arg1	quantification					731:744	longitudinal quantification	718:744	longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs	718:807	In this study, we introduce a contrast-enhanced computed tomography (CECT)-based method using a cationic iodinated contrast agent (CA4+) for longitudinal quantification of glycosaminoglycans (GAG) in cartilage-engineered constructs.					
31776943	0	0	theme	Subsequent	89:98	arg1	Treatment					111:119	Subsequent Microalgal Treatment	89:119	Subsequent Microalgal Treatment	89:119	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.					
31776943	3	1	theme	design	767:772	arg1	method					780:785	central composite design (CCD) method	749:785	central composite design (CCD) method	749:785	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	5	2	theme	Chlorella	1100:1108	arg1	cultivation					1119:1129	microalgae Chlorella vulgaris cultivation	1089:1129	microalgae Chlorella vulgaris cultivation	1089:1129	Applying 2-fold dilution, the supernatant from this treatment was further used for microalgae Chlorella vulgaris cultivation.					
31776943	5	3	theme	vulgaris	1110:1117	arg1	cultivation					1119:1129	microalgae Chlorella vulgaris cultivation	1089:1129	microalgae Chlorella vulgaris cultivation	1089:1129	Applying 2-fold dilution, the supernatant from this treatment was further used for microalgae Chlorella vulgaris cultivation.					
31776943	1	4	from	Idaho	174:178	arg1	concentrations					127:140	High concentrations	122:140	High concentrations of dairy operations in Southern Idaho	122:178	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	3	5	theme	Solid-liquid	549:560	arg1	separation					562:571	Solid-liquid separation	549:571	Solid-liquid separation	549:571	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	2	6	from	growth	496:501	arg1	part					517:520	the liquid part	506:520	the liquid part	506:520	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	7	7	theme	clear	1297:1301	arg1	solution					1303:1310	a relatively clear solution	1284:1310	a relatively clear solution that became suitable for photo-mixotrophic culture of microalgae	1284:1375	Turbidity was largely removed from the original dairy manure, providing a relatively clear solution that became suitable for photo-mixotrophic culture of microalgae.					
31776943	2	8	from	load	348:351	arg1	manure					362:367	dairy manure	356:367	dairy manure	356:367	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	3	9	theme	natural	617:623	arg1	chitosan					636:643	a natural flocculant chitosan	615:643	a natural flocculant chitosan	615:643	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	0	10	theme	Microalgal	100:109	arg1	Treatment					111:119	Subsequent Microalgal Treatment	89:119	Subsequent Microalgal Treatment	89:119	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.					
31776943	4	11	theme	flocculation-centrifuge	806:828	arg1	pretreatment					830:841	the optimal flocculation-centrifuge pretreatment	794:841	the optimal flocculation-centrifuge pretreatment	794:841	Under the optimal flocculation-centrifuge pretreatment, i.e., the maximal total suspended solid (turbidity) removal group, turbidity, COD, TKN, and TP have been removed by 92.7%, 72.6%, 58.7%, and 43.0%, respectively.					
31776943	2	12	theme	more	300:303	arg1	technologies					305:316	more technologies	300:316	more technologies confronting the high nutrient load in dairy manure	300:367	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	7	13	theme	photo-mixotrophic	1337:1353	arg1	culture					1355:1361	photo-mixotrophic culture	1337:1361	photo-mixotrophic culture of microalgae	1337:1375	Turbidity was largely removed from the original dairy manure, providing a relatively clear solution that became suitable for photo-mixotrophic culture of microalgae.					
31776943	4	14	theme	removal	896:902	arg1	group					904:908	the maximal total suspended solid (turbidity) removal group	850:908	the maximal total suspended solid (turbidity) removal group	850:908	Under the optimal flocculation-centrifuge pretreatment, i.e., the maximal total suspended solid (turbidity) removal group, turbidity, COD, TKN, and TP have been removed by 92.7%, 72.6%, 58.7%, and 43.0%, respectively.					
31776943	3	15	theme	flocculation-centrifuge	576:598	arg1	process					600:606	flocculation-centrifuge process	576:606	flocculation-centrifuge process	576:606	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	2	16	theme	enhanced	401:408	arg1	separation					423:432	enhanced solid-liquid separation	401:432	enhanced solid-liquid separation	401:432	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	7	17	theme	dairy	1260:1264	arg1	manure					1266:1271	the original dairy manure	1247:1271	the original dairy manure	1247:1271	Turbidity was largely removed from the original dairy manure, providing a relatively clear solution that became suitable for photo-mixotrophic culture of microalgae.					
31776943	7	18	theme	microalgae	1366:1375	arg1	culture					1355:1361	photo-mixotrophic culture	1337:1361	photo-mixotrophic culture of microalgae	1337:1375	Turbidity was largely removed from the original dairy manure, providing a relatively clear solution that became suitable for photo-mixotrophic culture of microalgae.					
31776943	2	19	theme	solid-liquid	410:421	arg1	separation					423:432	enhanced solid-liquid separation	401:432	enhanced solid-liquid separation	401:432	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	3	20	theme	CCD	775:777	arg1	method					780:785	central composite design (CCD) method	749:785	central composite design (CCD) method	749:785	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	1	21	from	operations	151:160	arg1	Idaho					174:178	Southern Idaho	165:178	Southern Idaho	165:178	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	2	22	theme	nutrient	339:346	arg1	load					348:351	the high nutrient load	330:351	the high nutrient load in dairy manure	330:367	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	1	23	theme	big	196:198	arg1	challenges					200:209	big challenges	196:209	big challenges for proper manure management	196:238	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	0	24	theme	Nutrient	0:7	arg1	Reduction					9:17	Nutrient Reduction	0:17	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.	0:120	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.					
31776943	1	25	from	concentrations	127:140	arg1	Idaho					174:178	Southern Idaho	165:178	Southern Idaho	165:178	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	2	26	theme	high	334:337	arg1	load					348:351	the high nutrient load	330:351	the high nutrient load in dairy manure	330:367	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	7	27	attach	removed	1234:1240	arg2	Turbidity					1212:1220	Turbidity	1212:1220	Turbidity	1212:1220	Turbidity was largely removed from the original dairy manure, providing a relatively clear solution that became suitable for photo-mixotrophic culture of microalgae.					
31776943	7	27	attach	removed	1234:1240	arg1	manure					1266:1271	the original dairy manure	1247:1271	the original dairy manure	1247:1271	Turbidity was largely removed from the original dairy manure, providing a relatively clear solution that became suitable for photo-mixotrophic culture of microalgae.					
31776943	0	28	theme	Dairy	22:26	arg1	Manure					28:33	Dairy Manure	22:33	Dairy Manure	22:33	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.					
31776943	4	29	theme	total	862:866	arg1	group					904:908	the maximal total suspended solid (turbidity) removal group	850:908	the maximal total suspended solid (turbidity) removal group	850:908	Under the optimal flocculation-centrifuge pretreatment, i.e., the maximal total suspended solid (turbidity) removal group, turbidity, COD, TKN, and TP have been removed by 92.7%, 72.6%, 58.7%, and 43.0%, respectively.					
31776943	4	30	theme	turbidity	885:893	arg1	group					904:908	the maximal total suspended solid (turbidity) removal group	850:908	the maximal total suspended solid (turbidity) removal group	850:908	Under the optimal flocculation-centrifuge pretreatment, i.e., the maximal total suspended solid (turbidity) removal group, turbidity, COD, TKN, and TP have been removed by 92.7%, 72.6%, 58.7%, and 43.0%, respectively.					
31776943	4	31	theme	solid	878:882	arg1	group					904:908	the maximal total suspended solid (turbidity) removal group	850:908	the maximal total suspended solid (turbidity) removal group	850:908	Under the optimal flocculation-centrifuge pretreatment, i.e., the maximal total suspended solid (turbidity) removal group, turbidity, COD, TKN, and TP have been removed by 92.7%, 72.6%, 58.7%, and 43.0%, respectively.					
31776943	3	32	dep	kaolin	668:673	arg1	aid					664:666	aid	664:666	aid	664:666	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	2	33	theme	subsequent	474:483	arg1	growth					496:501	the subsequent microalgal growth	470:501	the subsequent microalgal growth	470:501	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	3	34	theme	surface	707:713	arg1	RSM					728:730	RSM	728:730	RSM	728:730	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	3	34	theme	surface	707:713	arg1	methodology					715:725	response surface methodology	698:725	response surface methodology (RSM) technique using central composite design (CCD) method	698:785	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	1	35	theme	High	122:125	arg1	concentrations					127:140	High concentrations	122:140	High concentrations of dairy operations in Southern Idaho	122:178	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	1	36	theme	proper	215:220	arg1	management					229:238	proper manure management	215:238	proper manure management	215:238	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	3	37	theme	methodology	715:725	arg1	technique					733:741	response surface methodology (RSM) technique	698:741	response surface methodology (RSM) technique using central composite design (CCD) method	698:785	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	3	38	theme	flocculation	651:662	arg1	kaolin					668:673	a flocculation aid kaolin	649:673	a flocculation aid kaolin	649:673	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	7	39	theme	original	1251:1258	arg1	manure					1266:1271	the original dairy manure	1247:1271	the original dairy manure	1247:1271	Turbidity was largely removed from the original dairy manure, providing a relatively clear solution that became suitable for photo-mixotrophic culture of microalgae.					
31776943	1	40	theme	manure	222:227	arg1	management					229:238	proper manure management	215:238	proper manure management	215:238	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	0	41	theme	Manure	28:33	arg1	Reduction					9:17	Nutrient Reduction	0:17	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.	0:120	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.					
31776943	2	42	theme	microalgal	485:494	arg1	growth					496:501	the subsequent microalgal growth	470:501	the subsequent microalgal growth	470:501	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	2	43	theme	liquid	510:515	arg1	part					517:520	the liquid part	506:520	the liquid part	506:520	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	3	44	theme	response	698:705	arg1	RSM					728:730	RSM	728:730	RSM	728:730	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	3	44	theme	response	698:705	arg1	methodology					715:725	response surface methodology	698:725	response surface methodology (RSM) technique using central composite design (CCD) method	698:785	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	1	45	dep	brought	185:191	arg1	up					193:194	up	193:194	up	193:194	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	0	46	theme	Solid-Liquid	43:54	arg1	Separation					56:65	Solid-Liquid Separation	43:65	Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment	43:119	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.					
31776943	5	47	theme	microalgae	1089:1098	arg1	cultivation					1119:1129	microalgae Chlorella vulgaris cultivation	1089:1129	microalgae Chlorella vulgaris cultivation	1089:1129	Applying 2-fold dilution, the supernatant from this treatment was further used for microalgae Chlorella vulgaris cultivation.					
31776943	5	48	theme	2-fold	1015:1020	arg1	dilution					1022:1029	2-fold dilution	1015:1029	2-fold dilution	1015:1029	Applying 2-fold dilution, the supernatant from this treatment was further used for microalgae Chlorella vulgaris cultivation.					
31776943	1	49	theme	Southern	165:172	arg1	Idaho					174:178	Southern Idaho	165:178	Southern Idaho	165:178	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	2	50	theme	dairy	356:360	arg1	manure					362:367	dairy manure	356:367	dairy manure	356:367	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	3	51	theme	flocculant	625:634	arg1	chitosan					636:643	a natural flocculant chitosan	615:643	a natural flocculant chitosan	615:643	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	2	52	from	flocculation-centrifuge	442:464	arg1	part					517:520	the liquid part	506:520	the liquid part	506:520	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	4	53	theme	suspended	868:876	arg1	group					904:908	the maximal total suspended solid (turbidity) removal group	850:908	the maximal total suspended solid (turbidity) removal group	850:908	Under the optimal flocculation-centrifuge pretreatment, i.e., the maximal total suspended solid (turbidity) removal group, turbidity, COD, TKN, and TP have been removed by 92.7%, 72.6%, 58.7%, and 43.0%, respectively.					
31776943	1	54	theme	dairy	145:149	arg1	operations					151:160	dairy operations	145:160	dairy operations in Southern Idaho	145:178	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	4	55	theme	optimal	798:804	arg1	pretreatment					830:841	the optimal flocculation-centrifuge pretreatment	794:841	the optimal flocculation-centrifuge pretreatment	794:841	Under the optimal flocculation-centrifuge pretreatment, i.e., the maximal total suspended solid (turbidity) removal group, turbidity, COD, TKN, and TP have been removed by 92.7%, 72.6%, 58.7%, and 43.0%, respectively.					
31776943	0	56	with	Separation	56:65	arg1	Treatment					111:119	Subsequent Microalgal Treatment	89:119	Subsequent Microalgal Treatment	89:119	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.					
31776943	0	56	with	Separation	56:65	arg1	Flocculation					72:83	Flocculation	72:83	Flocculation	72:83	Nutrient Reduction of Dairy Manure Through Solid-Liquid Separation with Flocculation and Subsequent Microalgal Treatment.					
31776943	1	57	theme	operations	151:160	arg1	concentrations					127:140	High concentrations	122:140	High concentrations of dairy operations in Southern Idaho	122:178	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
31776943	5	58	from	treatment	1058:1066	arg1	supernatant					1036:1046	the supernatant	1032:1046	the supernatant from this treatment	1032:1066	Applying 2-fold dilution, the supernatant from this treatment was further used for microalgae Chlorella vulgaris cultivation.					
31776943	5	59	used	used	1080:1083	arg2	supernatant					1036:1046	the supernatant	1032:1046	the supernatant from this treatment	1032:1066	Applying 2-fold dilution, the supernatant from this treatment was further used for microalgae Chlorella vulgaris cultivation.					
31776943	3	60	theme	central	749:755	arg1	method					780:785	central composite design (CCD) method	749:785	central composite design (CCD) method	749:785	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	3	61	theme	composite	757:765	arg1	method					780:785	central composite design (CCD) method	749:785	central composite design (CCD) method	749:785	Solid-liquid separation by flocculation-centrifuge process, using a natural flocculant chitosan and a flocculation aid kaolin, was optimized through response surface methodology (RSM) technique using central composite design (CCD) method.					
31776943	4	62	theme	maximal	854:860	arg1	group					904:908	the maximal total suspended solid (turbidity) removal group	850:908	the maximal total suspended solid (turbidity) removal group	850:908	Under the optimal flocculation-centrifuge pretreatment, i.e., the maximal total suspended solid (turbidity) removal group, turbidity, COD, TKN, and TP have been removed by 92.7%, 72.6%, 58.7%, and 43.0%, respectively.					
31776943	2	63	theme	sequential	372:381	arg1	process					383:389	a sequential process	370:389	a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part	370:520	In order to explore more technologies confronting the high nutrient load in dairy manure, a sequential process combining enhanced solid-liquid separation through flocculation-centrifuge and the subsequent microalgal growth in the liquid part was studied in this study.					
31776943	1	64	theme	eutrophication	257:270	arg1	stress					272:277	the eutrophication stress	253:277	the eutrophication stress	253:277	High concentrations of dairy operations in Southern Idaho have brought up big challenges for proper manure management to alleviate the eutrophication stress.					
30953801	7	0	theme	electron	1628:1635	arg1	microscopy					1637:1646	transmission electron microscopy	1615:1646	transmission electron microscopy	1615:1646	Extrafibrillar demineralization was validated with transmission electron microscopy.					
30953801	4	1	theme	calcium	989:995	arg1	chelator					997:1004	an effective calcium chelator	976:1004	an effective calcium chelator	976:1004	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	4	1	theme	calcium	989:995	arg1	EDTA					970:973	EDTA	970:973	EDTA	970:973	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	10	2	theme	surface	2332:2338	arg1	layer					2340:2344	a surface layer	2330:2344	a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers	2330:2450	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	3	3	theme	resin	844:848	arg1	bonding					850:856	resin bonding	844:856	resin bonding	844:856	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	6	4	theme	strength	1368:1375	arg1	testing					1377:1383	bond strength testing	1363:1383	bond strength testing	1363:1383	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	3	5	dep	colonization	785:796	arg1	bonding					850:856	resin bonding	844:856	resin bonding	844:856	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	12	6	theme	biofilm	2751:2757	arg1	bacteria					2759:2766	biofilm bacteria	2751:2766	biofilm bacteria	2751:2766	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	10	7	theme	collagen	2388:2395	arg1	matrix					2397:2402	completely- or partially-demineralized collagen matrix	2349:2402	completely- or partially-demineralized collagen matrix	2349:2402	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	6	8	theme	collagen	1547:1554	arg1	matrix					1556:1561	the conditioned collagen matrix	1531:1561	the conditioned collagen matrix	1531:1561	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	7	9	theme	Extrafibrillar	1564:1577	arg1	demineralization					1579:1594	Extrafibrillar demineralization	1564:1594	Extrafibrillar demineralization	1564:1594	Extrafibrillar demineralization was validated with transmission electron microscopy.					
30953801	4	10	theme	EDTA	970:973	arg1	conjugation					955:965	conjugation	955:965	conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property	955:1086	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	13	11	theme	key	2840:2842	arg1	players					2857:2863	the key regenerative players	2836:2863	the key regenerative players within the dentin-pulp complex	2836:2894	The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex.					
30953801	3	12	theme	collagen	663:670	arg1	collapse					651:658	collapse	651:658	collapse of collagen that blocks resin infiltration after air-drying	651:718	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	4	13	theme	weight	1025:1030	arg1	chitosan					1039:1046	high molecular weight glycol chitosan	1010:1046	high molecular weight glycol chitosan	1010:1046	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	12	14	with	Conjugation	2558:2568	arg1	EDTA					2604:2607	EDTA	2604:2607	EDTA	2604:2607	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	13	15	theme	glycol	2795:2800	arg1	non-cytotoxic					2819:2831	non-cytotoxic	2819:2831	non-cytotoxic	2819:2831	The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex.					
30953801	13	15	theme	glycol	2795:2800	arg1	chitosan-EDTA					2802:2814	The high molecular weight glycol chitosan-EDTA	2769:2814	The high molecular weight glycol chitosan-EDTA	2769:2814	The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex.					
30953801	5	16	theme	isothermal	1152:1161	arg1	calorimetry					1173:1183	isothermal titration calorimetry	1152:1183	isothermal titration calorimetry	1152:1183	The GCE conjugate was purified, characterized by FTIR, 1H NMR, isothermal titration calorimetry and ICP-AES, and subjected to size exclusion dialysis to recover molecules that are >40 kDa.					
30953801	14	17	theme	hydrophobic	2945:2955	arg1	resins					2957:2962	hydrophobic resins	2945:2962	hydrophobic resins	2945:2962	This advance permits dry bonding and the use of hydrophobic resins.					
30953801	1	18	theme	precursor	266:274	arg1	inhibitors					276:285	mineralization precursor inhibitors	251:285	mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils	251:348	During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils.					
30953801	9	19	theme	dentin	1917:1922	arg1	conditioner					1924:1934	a dentin conditioner	1915:1934	a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique	1915:2083	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	9	19	theme	dentin	1917:1922	arg1	development					1900:1910	its ongoing development	1888:1910	its ongoing development	1888:1910	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	10	20	theme	monomers	2443:2450	arg1	infiltration					2412:2423	the infiltration	2408:2423	the infiltration of adhesive resin monomers	2408:2450	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	10	21	theme	mineralized	2241:2251	arg1	dentin					2279:2284	the mineralized, biofilm-covered, decayed dentin	2237:2284	the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers	2237:2450	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	6	22	theme	dentin	1404:1409	arg1	comparable					1432:1441	comparable	1432:1441	comparable	1432:1441	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	6	22	theme	dentin	1404:1409	arg1	results					1419:1425	the dentin bonding results	1400:1425	the dentin bonding results	1400:1425	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	4	23	theme	high	1010:1013	arg1	weight					1025:1030	high molecular weight	1010:1030	high molecular weight glycol chitosan	1010:1046	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	12	24	theme	chitosan	2590:2597	arg1	Conjugation					2558:2568	Conjugation	2558:2568	Conjugation of polymeric glycol chitosan with EDTA	2558:2607	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	6	25	theme	application	1308:1318	arg1	time					1320:1323	application time	1308:1323	application time for etching dentin	1308:1342	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	10	26	theme	adhesive	2428:2435	arg1	monomers					2443:2450	adhesive resin monomers	2428:2450	adhesive resin monomers	2428:2450	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	13	27	theme	molecular	2778:2786	arg1	weight					2788:2793	high molecular weight	2773:2793	The high molecular weight glycol chitosan-EDTA	2769:2814	The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex.					
30953801	14	28	theme	dry	2918:2920	arg1	bonding					2922:2928	dry bonding	2918:2928	dry bonding	2918:2928	This advance permits dry bonding and the use of hydrophobic resins.					
30953801	1	29	dep	40 kDa	308:313	arg1	larger					296:301	larger	296:301	larger	296:301	During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils.					
30953801	1	29	dep	40 kDa	308:313	arg1	than					303:306	than	303:306	than	303:306	During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils.					
30953801	6	30	theme	equivalent	1486:1495	arg1	strength					1502:1509	equivalent bond strength	1486:1509	equivalent bond strength	1486:1509	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	1	31	theme	collagenous	174:184	arg1	tissues					186:192	mineralized collagenous tissues	162:192	mineralized collagenous tissues	162:192	During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils.					
30953801	10	32	theme	plastic	2184:2190	arg1	materials					2206:2214	plastic tooth-colored materials	2184:2214	plastic tooth-colored materials	2184:2214	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	10	33	theme	resin	2305:2309	arg1	monomers					2311:2318	acid resin monomers	2300:2318	acid resin monomers	2300:2318	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	5	34	theme	exclusion	1220:1228	arg1	dialysis					1230:1237	size exclusion dialysis	1215:1237	size exclusion dialysis	1215:1237	The GCE conjugate was purified, characterized by FTIR, 1H NMR, isothermal titration calorimetry and ICP-AES, and subjected to size exclusion dialysis to recover molecules that are >40 kDa.					
30953801	9	35	theme	single-species	1852:1865	arg1	biofilms					1867:1874	planktonic and single-species biofilms	1837:1874	planktonic and single-species biofilms	1837:1874	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	12	36	theme	polymeric	2573:2581	arg1	chitosan					2590:2597	polymeric glycol chitosan	2573:2597	polymeric glycol chitosan	2573:2597	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	4	37	theme	weak	1064:1067	arg1	property					1079:1086	weak chelation property	1064:1086	weak chelation property	1064:1086	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	8	38	theme	endogenous	1663:1672	arg1	proteases					1681:1689	endogenous dentin proteases	1663:1689	endogenous dentin proteases	1663:1689	Inhibition of endogenous dentin proteases was confirmed using in-situ zymography.					
30953801	4	39	theme	water-soluble	883:895	arg1	conditioner					924:934	a water-soluble glycol chitosan-EDTA (GCE) conditioner	881:934	a water-soluble glycol chitosan-EDTA (GCE) conditioner	881:934	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	0	40	theme	mineralized	118:128	arg1	collagen					130:137	mineralized collagen	118:137	mineralized collagen	118:137	Polymer conjugation optimizes EDTA as a calcium-chelating agent that exclusively removes extrafibrillar minerals from mineralized collagen.					
30953801	8	41	theme	proteases	1681:1689	arg1	Inhibition					1649:1658	Inhibition	1649:1658	Inhibition of endogenous dentin proteases	1649:1689	Inhibition of endogenous dentin proteases was confirmed using in-situ zymography.					
30953801	2	42	theme	dentin	423:428	arg1	bonding					430:436	dentin bonding	423:436	dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact	423:571	Such a property is incorporated in the design of a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact.					
30953801	0	43	theme	Polymer	0:6	arg1	conjugation					8:18	Polymer conjugation	0:18	Polymer conjugation	0:18	Polymer conjugation optimizes EDTA as a calcium-chelating agent that exclusively removes extrafibrillar minerals from mineralized collagen.					
30953801	9	44	theme	bonds	2028:2032	arg1	durability					2014:2023	the durability	2010:2023	the durability of bonds created using the etch-and-rinse bonding technique	2010:2083	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	4	45	theme	present	866:872	arg1	study					874:878	the present study	862:878	the present study	862:878	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	9	46	theme	GCE	1749:1751	arg1	non-cytotoxic					1776:1788	non-cytotoxic	1776:1788	non-cytotoxic	1776:1788	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	9	46	theme	GCE	1749:1751	arg1	conditioner					1760:1770	The water-soluble GCE dentin conditioner	1731:1770	The water-soluble GCE dentin conditioner	1731:1770	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	2	47	theme	intrafibrillar	542:555	arg1	minerals					557:564	the intrafibrillar minerals	538:564	the intrafibrillar minerals	538:564	Such a property is incorporated in the design of a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact.					
30953801	8	48	theme	in-situ	1711:1717	arg1	zymography					1719:1728	in-situ zymography	1711:1728	in-situ zymography	1711:1728	Inhibition of endogenous dentin proteases was confirmed using in-situ zymography.					
30953801	3	49	theme	demineralized	749:761	arg1	fibrils					763:769	the completely demineralized fibrils	734:769	the completely demineralized fibrils from bacteria	734:783	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	2	50	theme	etch-and-rinse	445:458	arg1	technique					460:468	the etch-and-rinse technique	441:468	the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact	441:571	Such a property is incorporated in the design of a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact.					
30953801	9	51	theme	etch-and-rinse	2052:2065	arg1	technique					2075:2083	the etch-and-rinse bonding technique	2048:2083	the etch-and-rinse bonding technique	2048:2083	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	4	52	theme	chelation	1069:1077	arg1	property					1079:1086	weak chelation property	1064:1086	weak chelation property	1064:1086	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	0	53	theme	calcium-chelating	40:56	arg1	EDTA					30:33	EDTA	30:33	EDTA	30:33	Polymer conjugation optimizes EDTA as a calcium-chelating agent that exclusively removes extrafibrillar minerals from mineralized collagen.					
30953801	0	53	theme	calcium-chelating	40:56	arg1	agent					58:62	a calcium-chelating agent	38:62	a calcium-chelating agent that exclusively removes extrafibrillar minerals from mineralized collagen	38:137	Polymer conjugation optimizes EDTA as a calcium-chelating agent that exclusively removes extrafibrillar minerals from mineralized collagen.					
30953801	12	54	theme	protease	2721:2728	arg1	activities					2730:2739	endogenous protease activities	2710:2739	endogenous protease activities	2710:2739	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	4	55	theme	chitosan-EDTA	904:916	arg1	conditioner					924:934	a water-soluble glycol chitosan-EDTA (GCE) conditioner	881:934	a water-soluble glycol chitosan-EDTA (GCE) conditioner	881:934	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	3	56	theme	collagen	626:633	arg1	fibrils					635:641	collagen fibrils	626:641	collagen fibrils	626:641	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	10	57	theme	SIGNIFICANCE	2099:2110	arg1	STATEMENT					2086:2094	STATEMENT	2086:2094	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.	2086:2451	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	4	58	theme	GCE	919:921	arg1	conditioner					924:934	a water-soluble glycol chitosan-EDTA (GCE) conditioner	881:934	a water-soluble glycol chitosan-EDTA (GCE) conditioner	881:934	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	3	59	theme	complete	597:604	arg1	demineralization					606:621	complete demineralization	597:621	complete demineralization of collagen fibrils	597:641	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	7	60	theme	transmission	1615:1626	arg1	microscopy					1637:1646	transmission electron microscopy	1615:1646	transmission electron microscopy	1615:1646	Extrafibrillar demineralization was validated with transmission electron microscopy.					
30953801	10	61	theme	completely-	2349:2359	arg1	matrix					2397:2402	completely- or partially-demineralized collagen matrix	2349:2402	completely- or partially-demineralized collagen matrix	2349:2402	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	3	62	from	bacteria	776:783	arg1	fibrils					763:769	the completely demineralized fibrils	734:769	the completely demineralized fibrils from bacteria	734:783	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	3	63	theme	endogenous	817:826	arg1	proteases					828:836	endogenous proteases	817:836	endogenous proteases	817:836	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	13	64	theme	regenerative	2844:2855	arg1	players					2857:2863	the key regenerative players	2836:2863	the key regenerative players within the dentin-pulp complex	2836:2894	The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex.					
30953801	9	65	theme	planktonic	1837:1846	arg1	biofilms					1867:1874	planktonic and single-species biofilms	1837:1874	planktonic and single-species biofilms	1837:1874	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	12	66	theme	dentin	2640:2645	arg1	bonding					2647:2653	dentin bonding	2640:2653	dentin bonding	2640:2653	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	10	67	theme	state-of-the-art	2125:2140	arg1	techniques					2142:2151	The current state-of-the-art techniques	2113:2151	The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials	2113:2214	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	3	68	theme	fibrils	635:641	arg1	demineralization					606:621	complete demineralization	597:621	complete demineralization of collagen fibrils	597:641	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	4	69	theme	effective	979:987	arg1	chelator					997:1004	an effective calcium chelator	976:1004	an effective calcium chelator	976:1004	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	4	69	theme	effective	979:987	arg1	EDTA					970:973	EDTA	970:973	EDTA	970:973	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	10	70	theme	matrix	2397:2402	arg1	layer					2340:2344	a surface layer	2330:2344	a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers	2330:2450	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	10	71	dep	STATEMENT	2086:2094	arg1	require					2216:2222	require	2216:2222	require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers	2216:2450	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	6	72	theme	bond	1363:1366	arg1	testing					1377:1383	bond strength testing	1363:1383	bond strength testing	1363:1383	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	1	73	theme	intrafibrillar	195:208	arg1	mineralization					210:223	intrafibrillar mineralization	195:223	intrafibrillar mineralization	195:223	During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils.					
30953801	10	74	with	dentin	2279:2284	arg1	monomers					2311:2318	acid resin monomers	2300:2318	acid resin monomers	2300:2318	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	10	74	with	dentin	2279:2284	arg1	acids					2291:2295	acids	2291:2295	acids	2291:2295	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	10	75	theme	partially-demineralized	2364:2386	arg1	matrix					2397:2402	completely- or partially-demineralized collagen matrix	2349:2402	completely- or partially-demineralized collagen matrix	2349:2402	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	10	76	theme	decayed	2165:2171	arg1	teeth					2173:2177	filling decayed teeth	2157:2177	filling decayed teeth with plastic tooth-colored materials	2157:2214	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	13	77	theme	weight	2788:2793	arg1	non-cytotoxic					2819:2831	non-cytotoxic	2819:2831	non-cytotoxic	2819:2831	The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex.					
30953801	13	77	theme	weight	2788:2793	arg1	chitosan-EDTA					2802:2814	The high molecular weight glycol chitosan-EDTA	2769:2814	The high molecular weight glycol chitosan-EDTA	2769:2814	The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex.					
30953801	14	78	theme	resins	2957:2962	arg1	use					2938:2940	the use	2934:2940	the use of hydrophobic resins	2934:2962	This advance permits dry bonding and the use of hydrophobic resins.					
30953801	14	78	theme	resins	2957:2962	arg1	bonding					2922:2928	dry bonding	2918:2928	dry bonding	2918:2928	This advance permits dry bonding and the use of hydrophobic resins.					
30953801	4	79	theme	molecular	1015:1023	arg1	weight					1025:1030	high molecular weight	1010:1030	high molecular weight glycol chitosan	1010:1046	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	12	80	theme	new	2620:2622	arg1	conditioner					2624:2634	a new conditioner	2618:2634	a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria	2618:2766	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	10	81	theme	tooth-colored	2192:2204	arg1	materials					2206:2214	plastic tooth-colored materials	2184:2214	plastic tooth-colored materials	2184:2214	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	5	82	theme	titration	1163:1171	arg1	calorimetry					1173:1183	isothermal titration calorimetry	1152:1183	isothermal titration calorimetry	1152:1183	The GCE conjugate was purified, characterized by FTIR, 1H NMR, isothermal titration calorimetry and ICP-AES, and subjected to size exclusion dialysis to recover molecules that are >40 kDa.					
30953801	3	83	theme	resin	684:688	arg1	infiltration					690:701	resin infiltration	684:701	resin infiltration after air-drying	684:718	This strategy prevents complete demineralization of collagen fibrils, avoids collapse of collagen that blocks resin infiltration after air-drying, and protects the completely demineralized fibrils from bacteria colonization and degradation by endogenous proteases after resin bonding.					
30953801	1	84	theme	mineralization	251:264	arg1	inhibitors					276:285	mineralization precursor inhibitors	251:285	mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils	251:348	During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils.					
30953801	4	85	theme	glycol	1032:1037	arg1	chitosan					1039:1046	high molecular weight glycol chitosan	1010:1046	high molecular weight glycol chitosan	1010:1046	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	6	86	theme	etching	1329:1335	arg1	dentin					1337:1342	etching dentin	1329:1342	etching dentin	1329:1342	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	9	87	contain	possessed	1794:1802	arg2	activities					1818:1827	antibacterial activities	1804:1827	antibacterial activities against planktonic and single-species biofilms	1804:1874	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	9	87	contain	possessed	1794:1802	arg1	non-cytotoxic					1776:1788	non-cytotoxic	1776:1788	non-cytotoxic	1776:1788	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	9	87	contain	possessed	1794:1802	arg1	conditioner					1760:1770	The water-soluble GCE dentin conditioner	1731:1770	The water-soluble GCE dentin conditioner	1731:1770	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	6	88	theme	bonding	1411:1417	arg1	comparable					1432:1441	comparable	1432:1441	comparable	1432:1441	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	6	88	theme	bonding	1411:1417	arg1	results					1419:1425	the dentin bonding results	1400:1425	the dentin bonding results	1400:1425	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	9	89	theme	ongoing	1892:1898	arg1	conditioner					1924:1934	a dentin conditioner	1915:1934	a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique	1915:2083	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	9	89	theme	ongoing	1892:1898	arg1	development					1900:1910	its ongoing development	1888:1910	its ongoing development	1888:1910	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	10	90	theme	resin	2437:2441	arg1	monomers					2443:2450	adhesive resin monomers	2428:2450	adhesive resin monomers	2428:2450	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	13	91	theme	high	2773:2776	arg1	weight					2788:2793	high molecular weight	2773:2793	The high molecular weight glycol chitosan-EDTA	2769:2814	The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex.					
30953801	5	92	theme	1H	1144:1145	arg1	NMR					1147:1149	1H NMR	1144:1149	1H NMR	1144:1149	The GCE conjugate was purified, characterized by FTIR, 1H NMR, isothermal titration calorimetry and ICP-AES, and subjected to size exclusion dialysis to recover molecules that are >40 kDa.					
30953801	9	93	theme	air-drying	1941:1950	arg1	properties					1988:1997	air-drying, anti-proteolytic and antibacterial properties	1941:1997	air-drying, anti-proteolytic and antibacterial properties	1941:1997	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	6	94	theme	bond	1497:1500	arg1	strength					1502:1509	equivalent bond strength	1486:1509	equivalent bond strength	1486:1509	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	1	95	theme	mineralized	162:172	arg1	tissues					186:192	mineralized collagenous tissues	162:192	mineralized collagenous tissues	162:192	During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils.					
30953801	9	96	theme	anti-proteolytic	1953:1968	arg1	properties					1988:1997	air-drying, anti-proteolytic and antibacterial properties	1941:1997	air-drying, anti-proteolytic and antibacterial properties	1941:1997	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	10	97	dep	mineralized	2241:2251	arg1	decayed					2271:2277	decayed	2271:2277	decayed	2271:2277	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	10	97	dep	mineralized	2241:2251	arg1	biofilm-covered					2254:2268	biofilm-covered	2254:2268	biofilm-covered	2254:2268	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	6	98	theme	optimal	1282:1288	arg1	concentration					1290:1302	The optimal concentration	1278:1302	The optimal concentration	1278:1302	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	0	99	theme	extrafibrillar	89:102	arg1	minerals					104:111	extrafibrillar minerals	89:111	extrafibrillar minerals	89:111	Polymer conjugation optimizes EDTA as a calcium-chelating agent that exclusively removes extrafibrillar minerals from mineralized collagen.					
30953801	5	100	theme	size	1215:1218	arg1	exclusion					1220:1228	size exclusion	1215:1228	size exclusion dialysis	1215:1237	The GCE conjugate was purified, characterized by FTIR, 1H NMR, isothermal titration calorimetry and ICP-AES, and subjected to size exclusion dialysis to recover molecules that are >40 kDa.					
30953801	1	101	theme	tissues	186:192	arg1	development					147:157	development	147:157	development of mineralized collagenous tissues	147:192	During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils.					
30953801	9	102	theme	antibacterial	1974:1986	arg1	properties					1988:1997	air-drying, anti-proteolytic and antibacterial properties	1941:1997	air-drying, anti-proteolytic and antibacterial properties	1941:1997	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	12	103	theme	glycol	2583:2588	arg1	chitosan					2590:2597	polymeric glycol chitosan	2573:2597	polymeric glycol chitosan	2573:2597	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	10	104	theme	acid	2300:2303	arg1	monomers					2311:2318	acid resin monomers	2300:2318	acid resin monomers	2300:2318	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	8	105	theme	dentin	1674:1679	arg1	proteases					1681:1689	endogenous dentin proteases	1663:1689	endogenous dentin proteases	1663:1689	Inhibition of endogenous dentin proteases was confirmed using in-situ zymography.					
30953801	6	106	theme	acid	1457:1460	arg1	etching					1462:1468	phosphoric acid etching	1446:1468	phosphoric acid etching	1446:1468	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	6	107	theme	matrix	1556:1561	arg1	air-drying					1517:1526	air-drying	1517:1526	air-drying of the conditioned collagen matrix	1517:1561	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	2	108	theme	extrafibrillar	501:514	arg1	apatite					516:522	extrafibrillar apatite	501:522	extrafibrillar apatite	501:522	Such a property is incorporated in the design of a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact.					
30953801	9	109	with	conditioner	1924:1934	arg1	properties					1988:1997	air-drying, anti-proteolytic and antibacterial properties	1941:1997	air-drying, anti-proteolytic and antibacterial properties	1941:1997	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	6	110	theme	conditioned	1535:1545	arg1	matrix					1556:1561	the conditioned collagen matrix	1531:1561	the conditioned collagen matrix	1531:1561	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	1	111	theme	collagen	333:340	arg1	fibrils					342:348	the collagen fibrils	329:348	the collagen fibrils	329:348	During development of mineralized collagenous tissues, intrafibrillar mineralization is achieved by preventing mineralization precursor inhibitors that are larger than 40 kDa from entering the collagen fibrils.					
30953801	9	112	theme	water-soluble	1735:1747	arg1	non-cytotoxic					1776:1788	non-cytotoxic	1776:1788	non-cytotoxic	1776:1788	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	9	112	theme	water-soluble	1735:1747	arg1	conditioner					1760:1770	The water-soluble GCE dentin conditioner	1731:1770	The water-soluble GCE dentin conditioner	1731:1770	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	10	113	with	teeth	2173:2177	arg1	materials					2206:2214	plastic tooth-colored materials	2184:2214	plastic tooth-colored materials	2184:2214	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	2	114	theme	chelator	410:417	arg1	design					390:395	the design	386:395	the design of a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact	386:571	Such a property is incorporated in the design of a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact.					
30953801	13	115	theme	dentin-pulp	2876:2886	arg1	complex					2888:2894	the dentin-pulp complex	2872:2894	the dentin-pulp complex	2872:2894	The high molecular weight glycol chitosan-EDTA is non-cytotoxic to the key regenerative players within the dentin-pulp complex.					
30953801	9	116	theme	dentin	1753:1758	arg1	non-cytotoxic					1776:1788	non-cytotoxic	1776:1788	non-cytotoxic	1776:1788	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	9	116	theme	dentin	1753:1758	arg1	conditioner					1760:1770	The water-soluble GCE dentin conditioner	1731:1770	The water-soluble GCE dentin conditioner	1731:1770	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	12	117	theme	endogenous	2710:2719	arg1	activities					2730:2739	endogenous protease activities	2710:2739	endogenous protease activities	2710:2739	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	6	118	theme	phosphoric	1446:1455	arg1	acid					1457:1460	phosphoric acid	1446:1460	phosphoric acid etching	1446:1468	The optimal concentration and application time for etching dentin were determined by bond strength testing to ensure that the dentin bonding results were comparable to phosphoric acid etching, and maintained equivalent bond strength after air-drying of the conditioned collagen matrix.					
30953801	9	119	theme	bonding	2067:2073	arg1	technique					2075:2083	the etch-and-rinse bonding technique	2048:2083	the etch-and-rinse bonding technique	2048:2083	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	10	120	theme	current	2117:2123	arg1	techniques					2142:2151	The current state-of-the-art techniques	2113:2151	The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials	2113:2214	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	5	121	theme	GCE	1093:1095	arg1	conjugate					1097:1105	The GCE conjugate	1089:1105	The GCE conjugate	1089:1105	The GCE conjugate was purified, characterized by FTIR, 1H NMR, isothermal titration calorimetry and ICP-AES, and subjected to size exclusion dialysis to recover molecules that are >40 kDa.					
30953801	2	122	from	bonding	430:436	arg1	technique					460:468	the etch-and-rinse technique	441:468	the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact	441:571	Such a property is incorporated in the design of a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact.					
30953801	12	123	theme	extrafibrillar	2679:2692	arg1	dentin					2694:2699	only extrafibrillar dentin	2674:2699	only extrafibrillar dentin	2674:2699	Conjugation of polymeric glycol chitosan with EDTA produces a new conditioner for dentin bonding that demineralizes only extrafibrillar dentin, reduces endogenous protease activities and kills biofilm bacteria.					
30953801	4	124	theme	glycol	897:902	arg1	conditioner					924:934	a water-soluble glycol chitosan-EDTA (GCE) conditioner	881:934	a water-soluble glycol chitosan-EDTA (GCE) conditioner	881:934	In the present study, a water-soluble glycol chitosan-EDTA (GCE) conditioner was synthesized by conjugation of EDTA, an effective calcium chelator, to high molecular weight glycol chitosan, which exhibits weak chelation property.					
30953801	9	125	theme	antibacterial	1804:1816	arg1	activities					1818:1827	antibacterial activities	1804:1827	antibacterial activities against planktonic and single-species biofilms	1804:1874	The water-soluble GCE dentin conditioner was non-cytotoxic and possessed antibacterial activities against planktonic and single-species biofilms, supporting its ongoing development as a dentin conditioner with air-drying, anti-proteolytic and antibacterial properties to enhance the durability of bonds created using the etch-and-rinse bonding technique.					
30953801	14	126	dep	This	2897:2900	arg1	advance					2902:2908	advance	2902:2908	advance	2902:2908	This advance permits dry bonding and the use of hydrophobic resins.					
30953801	10	127	theme	filling	2157:2163	arg1	teeth					2173:2177	filling decayed teeth	2157:2177	filling decayed teeth with plastic tooth-colored materials	2157:2214	STATEMENT OF SIGNIFICANCE: The current state-of-the-art techniques for filling decayed teeth with plastic tooth-colored materials require conditioning the mineralized, biofilm-covered, decayed dentin with acids or acid resin monomers to create a surface layer of completely- or partially-demineralized collagen matrix for the infiltration of adhesive resin monomers.					
30953801	2	128	theme	calcium	402:408	arg1	chelator					410:417	a calcium chelator	400:417	a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact	400:571	Such a property is incorporated in the design of a calcium chelator for dentin bonding in the etch-and-rinse technique that selectively demineralizes extrafibrillar apatite while leaving the intrafibrillar minerals intact.					
30614308	6	0	theme	Statistical	1024:1034	arg1	analyses					1036:1043	Statistical analyses	1024:1043	Statistical analyses	1024:1043	Statistical analyses were performed with one-way ANOVA, followed by the Bonferroni test.					
30614308	8	1	from	cycle	1421:1425	arg1	phase					1448:1452	the proliferative phase	1430:1452	the proliferative phase	1430:1452	We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.					
30614308	4	2	theme	assay	845:849	arg1	method					818:823	the biochemical method	802:823	the biochemical method of the fluorimetric assay (ELISA-like)	802:862	Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like).					
30614308	3	3	theme	Hospital	690:697	arg1	Division					669:676	Gynecology Division	658:676	Gynecology Division of Clinical Hospital of the FMUSP (HC-USP)	658:719	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	1	4	theme	cellular	167:174	arg1	levels					176:181	different cellular levels	157:181	different cellular levels	157:181	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	7	5	from	reactivity	1209:1218	arg1	endometrium					1232:1242	the PCOS endometrium	1223:1242	the PCOS endometrium	1223:1242	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	3	6	theme	endometrial	528:538	arg1	samples					540:546	60 endometrial samples	525:546	60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase	525:643	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	1	7	theme	cell	189:192	arg1	death					194:198	cell death	189:198	cell death	189:198	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	5	8	theme	western	1006:1012	arg1	blotting					1014:1021	western blotting	1006:1021	western blotting	1006:1021	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	8	9	theme	acid	1391:1394	arg1	composition					1365:1375	different composition	1355:1375	different composition of hyaluronic acid	1355:1394	We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.					
30614308	2	10	with	patients	443:450	arg1	PCOS					484:487	PCOS	484:487	PCOS	484:487	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	10	with	patients	443:450	arg1	syndrome					474:481	polycystic ovary syndrome	457:481	polycystic ovary syndrome (PCOS)	457:488	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	1	11	theme	wide	132:135	arg1	range					137:141	a wide range	130:141	a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes	130:303	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	7	12	theme	regular	1267:1273	arg1	cycles					1285:1290	regular menstrual cycles	1267:1290	regular menstrual cycles	1267:1290	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	7	13	theme	higher	1162:1167	arg1	reactivity					1209:1218	a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity	1160:1218	a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles	1160:1290	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	8	14	contain	have	1350:1353	arg2	composition					1365:1375	different composition	1355:1375	different composition of hyaluronic acid	1355:1394	We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.					
30614308	8	14	contain	have	1350:1353	arg1	patients					1341:1348	PCOS patients	1336:1348	PCOS patients	1336:1348	We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.					
30614308	2	15	from	synthases	411:419	arg1	endometrium					428:438	the endometrium	424:438	the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	424:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	0	16	theme	polycystic	61:70	arg1	syndrome					78:85	polycystic ovary syndrome	61:85	polycystic ovary syndrome	61:85	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome.					
30614308	2	17	theme	women	507:511	arg1	endometrium					428:438	the endometrium	424:438	the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	424:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	0	18	from	Analysis	0:7	arg1	endometrium					35:45	the endometrium	31:45	the endometrium of women with polycystic ovary syndrome	31:85	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome.					
30614308	1	19	from	levels	176:181	arg1	signals					146:152	signals	146:152	signals	146:152	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	1	19	from	levels	176:181	arg1	range					137:141	a wide range	130:141	a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes	130:303	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	1	20	from	range	137:141	arg1	levels					176:181	different cellular levels	157:181	different cellular levels	157:181	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	0	21	from	endometrium	35:45	arg1	Analysis					0:7	Analysis	0:7	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome	0:85	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome.					
30614308	7	22	theme	acid	1134:1137	arg1	synthases					1139:1147	hyaluronic acid synthases	1123:1147	hyaluronic acid synthases	1123:1147	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	2	23	with	women	507:511	arg1	PCOS					484:487	PCOS	484:487	PCOS	484:487	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	23	with	women	507:511	arg1	syndrome					474:481	polycystic ovary syndrome	457:481	polycystic ovary syndrome (PCOS)	457:488	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	3	24	from	patients	556:563	arg1	total					516:520	A total	514:520	A total	514:520	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	3	24	from	patients	556:563	arg1	samples					540:546	60 endometrial samples	525:546	60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase	525:643	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	2	25	theme	acid	406:409	arg1	synthases					411:419	hyaluronic acid synthases	395:419	hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	395:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	0	26	theme	hyaluronic	12:21	arg1	acid					23:26	hyaluronic acid	12:26	hyaluronic acid in the endometrium of women with polycystic ovary syndrome	12:85	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome.					
30614308	1	27	theme	extracellular	100:112	arg1	matrix					114:119	Endometrium extracellular matrix	88:119	Endometrium extracellular matrix	88:119	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	2	28	theme	polycystic	457:466	arg1	PCOS					484:487	PCOS	484:487	PCOS	484:487	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	28	theme	polycystic	457:466	arg1	syndrome					474:481	polycystic ovary syndrome	457:481	polycystic ovary syndrome (PCOS)	457:488	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	0	29	theme	women	50:54	arg1	endometrium					35:45	the endometrium	31:45	the endometrium of women with polycystic ovary syndrome	31:85	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome.					
30614308	4	30	theme	acid	780:783	arg1	concentration					752:764	the concentration	748:764	the concentration of hyaluronic acid	748:783	Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like).					
30614308	4	30	theme	acid	780:783	arg1	determination					730:742	Profile determination	722:742	Profile determination	722:742	Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like).					
30614308	1	31	theme	proliferative	281:293	arg1	processes					295:303	the proliferative processes	277:303	the proliferative processes	277:303	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	2	32	theme	patients	443:450	arg1	endometrium					428:438	the endometrium	424:438	the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	424:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	3	33	theme	menstrual	601:609	arg1	cycles					611:616	regular menstrual cycles	593:616	regular menstrual cycles in the proliferative phase	593:643	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	8	34	theme	PCOS	1336:1339	arg1	patients					1341:1348	PCOS patients	1336:1348	PCOS patients	1336:1348	We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.					
30614308	2	35	theme	study	328:332	arg1	objective					310:318	The objective	306:318	The objective of this study	306:332	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	6	36	theme	one-way	1065:1071	arg1	ANOVA					1073:1077	one-way ANOVA	1065:1077	one-way ANOVA	1065:1077	Statistical analyses were performed with one-way ANOVA, followed by the Bonferroni test.					
30614308	5	37	theme	synthases	937:945	arg1	location					869:876	Its location	865:876	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3)	865:967	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	5	37	theme	synthases	937:945	arg1	dosage					919:924	the dosage	915:924	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3)	865:967	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	7	38	theme	lower	1198:1202	arg1	reactivity					1209:1218	a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity	1160:1218	a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles	1160:1290	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	3	39	theme	proliferative	625:637	arg1	phase					639:643	the proliferative phase	621:643	the proliferative phase	621:643	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	2	40	theme	hyaluronic	349:358	arg1	concentration					365:377	hyaluronic acid concentration	349:377	hyaluronic acid concentration	349:377	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	40	theme	hyaluronic	349:358	arg1	enzymes					384:390	the enzymes	380:390	the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	380:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	3	41	with	women	582:586	arg1	cycles					611:616	regular menstrual cycles	593:616	regular menstrual cycles in the proliferative phase	593:643	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	3	41	with	women	582:586	arg1	PCOS					570:573	PCOS	570:573	PCOS	570:573	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	4	42	theme	fluorimetric	832:843	arg1	ELISA-like					852:861	ELISA-like	852:861	ELISA-like	852:861	Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like).					
30614308	4	42	theme	fluorimetric	832:843	arg1	assay					845:849	the fluorimetric assay	828:849	the fluorimetric assay (ELISA-like)	828:862	Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like).					
30614308	3	43	from	cycles	611:616	arg1	phase					639:643	the proliferative phase	621:643	the proliferative phase	621:643	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	0	44	with	women	50:54	arg1	syndrome					78:85	polycystic ovary syndrome	61:85	polycystic ovary syndrome	61:85	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome.					
30614308	1	45	theme	different	157:165	arg1	levels					176:181	different cellular levels	157:181	different cellular levels	157:181	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	6	46	theme	Bonferroni	1096:1105	arg1	test					1107:1110	the Bonferroni test	1092:1110	the Bonferroni test	1092:1110	Statistical analyses were performed with one-way ANOVA, followed by the Bonferroni test.					
30614308	3	47	theme	Clinical	681:688	arg1	Hospital					690:697	Clinical Hospital	681:697	Clinical Hospital of the FMUSP (HC-USP)	681:719	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	3	48	theme	samples	540:546	arg1	total					516:520	A total	514:520	A total	514:520	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	8	49	theme	proliferative	1434:1446	arg1	phase					1448:1452	the proliferative phase	1430:1452	the proliferative phase	1430:1452	We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.					
30614308	4	50	theme	biochemical	806:816	arg1	method					818:823	the biochemical method	802:823	the biochemical method of the fluorimetric assay (ELISA-like)	802:862	Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like).					
30614308	8	51	theme	hyaluronic	1380:1389	arg1	acid					1391:1394	hyaluronic acid	1380:1394	hyaluronic acid	1380:1394	We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.					
30614308	3	52	theme	FMUSP	706:710	arg1	Hospital					690:697	Clinical Hospital	681:697	Clinical Hospital of the FMUSP (HC-USP)	681:719	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	5	53	dep	synthases	937:945	arg1	HAS2					954:957	HAS2	954:957	HAS2	954:957	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	5	53	dep	synthases	937:945	arg1	HAS1					948:951	HAS1	948:951	HAS1	948:951	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	5	53	dep	synthases	937:945	arg1	synthases					937:945	enzymes synthases	929:945	enzymes synthases (HAS1, HAS2 and HAS3)	929:967	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	5	53	dep	synthases	937:945	arg1	HAS3					963:966	HAS3	963:966	HAS3	963:966	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	7	54	theme	proliferative	1299:1311	arg1	phase					1313:1317	the proliferative phase	1295:1317	the proliferative phase	1295:1317	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	7	55	with	women	1256:1260	arg1	cycles					1285:1290	regular menstrual cycles	1267:1290	regular menstrual cycles	1267:1290	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	8	56	theme	regular	1413:1419	arg1	cycle					1421:1425	a regular cycle	1411:1425	a regular cycle in the proliferative phase	1411:1452	We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.					
30614308	0	57	from	acid	23:26	arg1	endometrium					35:45	the endometrium	31:45	the endometrium of women with polycystic ovary syndrome	31:85	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome.					
30614308	7	58	theme	menstrual	1275:1283	arg1	cycles					1285:1290	regular menstrual cycles	1267:1290	regular menstrual cycles	1267:1290	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	3	59	with	patients	556:563	arg1	cycles					611:616	regular menstrual cycles	593:616	regular menstrual cycles in the proliferative phase	593:643	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	3	59	with	patients	556:563	arg1	PCOS					570:573	PCOS	570:573	PCOS	570:573	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	0	60	theme	ovary	72:76	arg1	syndrome					78:85	polycystic ovary syndrome	61:85	polycystic ovary syndrome	61:85	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome.					
30614308	1	61	theme	signals	146:152	arg1	range					137:141	a wide range	130:141	a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes	130:303	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	2	62	theme	ovary	468:472	arg1	PCOS					484:487	PCOS	484:487	PCOS	484:487	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	62	theme	ovary	468:472	arg1	syndrome					474:481	polycystic ovary syndrome	457:481	polycystic ovary syndrome (PCOS)	457:488	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	63	from	endometrium	428:438	arg1	concentration					365:377	hyaluronic acid concentration	349:377	hyaluronic acid concentration	349:377	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	63	from	endometrium	428:438	arg1	enzymes					384:390	the enzymes	380:390	the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	380:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	3	64	from	total	516:520	arg1	women					582:586	30 women	579:586	30 women	579:586	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	3	64	from	total	516:520	arg1	patients					556:563	30 patients	553:563	30 patients with PCOS	553:573	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	2	65	theme	eumenorrheic	494:505	arg1	women					507:511	eumenorrheic women	494:511	eumenorrheic women	494:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	7	66	theme	hyaluronic	1123:1132	arg1	acid					1134:1137	hyaluronic acid	1123:1137	hyaluronic acid synthases	1123:1147	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	4	67	theme	Profile	722:728	arg1	determination					730:742	Profile determination	722:742	Profile determination	722:742	Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like).					
30614308	3	68	from	women	582:586	arg1	total					516:520	A total	514:520	A total	514:520	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	3	68	from	women	582:586	arg1	samples					540:546	60 endometrial samples	525:546	60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase	525:643	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	5	69	theme	endometrial	885:895	arg1	tissue					897:902	the endometrial tissue	881:902	the endometrial tissue	881:902	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	2	70	theme	acid	360:363	arg1	concentration					365:377	hyaluronic acid concentration	349:377	hyaluronic acid concentration	349:377	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	70	theme	acid	360:363	arg1	enzymes					384:390	the enzymes	380:390	the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	380:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	0	71	theme	acid	23:26	arg1	Analysis					0:7	Analysis	0:7	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome	0:85	Analysis of hyaluronic acid in the endometrium of women with polycystic ovary syndrome.					
30614308	5	72	from	location	869:876	arg1	tissue					897:902	the endometrial tissue	881:902	the endometrial tissue	881:902	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	1	73	theme	Endometrium	88:98	arg1	matrix					114:119	Endometrium extracellular matrix	88:119	Endometrium extracellular matrix	88:119	Endometrium extracellular matrix provides a wide range of signals at different cellular levels, like cell death and proliferation, which can be important for regulating menses and reducing the proliferative processes.					
30614308	7	74	theme	PCOS	1227:1230	arg1	endometrium					1232:1242	the PCOS endometrium	1223:1242	the PCOS endometrium	1223:1242	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	2	75	theme	synthases	411:419	arg1	concentration					365:377	hyaluronic acid concentration	349:377	hyaluronic acid concentration	349:377	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	75	theme	synthases	411:419	arg1	enzymes					384:390	the enzymes	380:390	the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	380:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	2	76	theme	hyaluronic	395:404	arg1	acid					406:409	hyaluronic acid	395:409	hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	395:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	7	77	dep	higher	1162:1167	arg1	HAS1					1169:1172	HAS1	1169:1172	HAS1	1169:1172	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	7	77	dep	higher	1162:1167	arg1	reactivity					1183:1192	HAS2 reactivity	1178:1192	HAS2 reactivity	1178:1192	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	3	78	theme	regular	593:599	arg1	cycles					611:616	regular menstrual cycles	593:616	regular menstrual cycles in the proliferative phase	593:643	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	4	79	theme	hyaluronic	769:778	arg1	acid					780:783	hyaluronic acid	769:783	hyaluronic acid	769:783	Profile determination and the concentration of hyaluronic acid was performed by the biochemical method of the fluorimetric assay (ELISA-like).					
30614308	7	80	theme	HAS3	1204:1207	arg1	reactivity					1209:1218	a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity	1160:1218	a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles	1160:1290	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	5	81	theme	enzymes	929:935	arg1	HAS2					954:957	HAS2	954:957	HAS2	954:957	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	5	81	theme	enzymes	929:935	arg1	HAS1					948:951	HAS1	948:951	HAS1	948:951	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	5	81	theme	enzymes	929:935	arg1	synthases					937:945	enzymes synthases	929:945	enzymes synthases (HAS1, HAS2 and HAS3)	929:967	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	5	81	theme	enzymes	929:935	arg1	HAS3					963:966	HAS3	963:966	HAS3	963:966	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30614308	6	82	dep	performed	1050:1058	arg1	followed					1080:1087	followed	1080:1087	followed by the Bonferroni test	1080:1110	Statistical analyses were performed with one-way ANOVA, followed by the Bonferroni test.					
30614308	2	83	from	enzymes	384:390	arg1	endometrium					428:438	the endometrium	424:438	the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women	424:511	The objective of this study is to evaluate hyaluronic acid concentration, the enzymes of hyaluronic acid synthases in the endometrium of patients with polycystic ovary syndrome (PCOS) and eumenorrheic women.					
30614308	7	84	theme	HAS2	1178:1181	arg1	reactivity					1183:1192	HAS2 reactivity	1178:1192	HAS2 reactivity	1178:1192	Regarding hyaluronic acid synthases, there was a higher HAS1 and HAS2 reactivity and lower HAS3 reactivity in the PCOS endometrium compared to women with regular menstrual cycles in the proliferative phase.					
30614308	3	85	theme	Gynecology	658:667	arg1	Division					669:676	Gynecology Division	658:676	Gynecology Division of Clinical Hospital of the FMUSP (HC-USP)	658:719	A total of 60 endometrial samples from 30 patients with PCOS and 30 women with regular menstrual cycles in the proliferative phase, attended at Gynecology Division of Clinical Hospital of the FMUSP (HC-USP).					
30614308	8	86	theme	different	1355:1363	arg1	composition					1365:1375	different composition	1355:1375	different composition of hyaluronic acid	1355:1394	We suggest that PCOS patients have different composition of hyaluronic acid in relation to a regular cycle in the proliferative phase.					
30614308	5	87	from	dosage	919:924	arg1	tissue					897:902	the endometrial tissue	881:902	the endometrial tissue	881:902	Its location in the endometrial tissue as well as the dosage of enzymes synthases (HAS1, HAS2 and HAS3) was done by immunohistochemistry and western blotting.					
30818812	6	0	dep	hazelnut	779:786	arg1	macadamia					805:813	macadamia	805:813	macadamia	805:813	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	6	0	dep	hazelnut	779:786	arg1	pistachio					820:828	pistachio	820:828	pistachio	820:828	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	6	0	dep	hazelnut	779:786	arg1	walnut					797:802	walnut	797:802	walnut	797:802	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	6	0	dep	hazelnut	779:786	arg1	almond					789:794	almond	789:794	almond	789:794	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	11	1	theme	consumption	1369:1379	arg1	contribution					1349:1360	a beneficial contribution	1336:1360	a beneficial contribution of nut consumption to inflammatory diseases	1336:1404	Hence, a beneficial contribution of nut consumption to inflammatory diseases can be assumed.					
30818812	9	2	theme	nitric	1155:1160	arg1	oxide					1162:1166	reduced nitric oxide	1147:1166	reduced nitric oxide formation	1147:1176	iNos protein expression was down-regulated followed by reduced nitric oxide formation.					
30818812	7	3	theme	main	886:889	arg1	acid					897:900	the main fatty acid	882:900	the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts	882:955	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	7	3	theme	main	886:889	arg1	acid					873:876	Oleic acid	867:876	Oleic acid	867:876	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	10	4	theme	nut	1185:1187	arg1	extracts					1189:1196	nut extracts	1185:1196	nut extracts at concentrations achievable in the digestive tract	1185:1248	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	5	5	theme	fat-soluble	594:604	arg1	constituents					606:617	the fat-soluble constituents	590:617	the fat-soluble constituents	590:617	We therefore prepared oily nut extracts after in vitro digestion and saponification of the fat-soluble constituents.					
30818812	8	6	theme	nut	968:970	arg1	extracts					972:979	oily nut extracts	963:979	oily nut extracts	963:979	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	10	7	from	concentrations	1201:1214	arg1	extracts					1189:1196	nut extracts	1185:1196	nut extracts at concentrations achievable in the digestive tract	1185:1248	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	9	8	theme	reduced	1147:1153	arg1	oxide					1162:1166	reduced nitric oxide	1147:1166	reduced nitric oxide formation	1147:1176	iNos protein expression was down-regulated followed by reduced nitric oxide formation.					
30818812	4	9	from	effects	420:426	arg1	processes					460:468	inflammatory processes	447:468	inflammatory processes	447:468	However, studies investigating the effects of nut extracts on inflammatory processes on the molecular level are rare.					
30818812	4	9	from	effects	420:426	arg1	level					487:491	the molecular level	473:491	the molecular level	473:491	However, studies investigating the effects of nut extracts on inflammatory processes on the molecular level are rare.					
30818812	5	10	theme	oily	525:528	arg1	extracts					534:541	oily nut extracts	525:541	oily nut extracts	525:541	We therefore prepared oily nut extracts after in vitro digestion and saponification of the fat-soluble constituents.					
30818812	1	11	theme	inflammatory	173:184	arg1	diseases					186:193	inflammatory diseases	173:193	inflammatory diseases	173:193	Nut consumption is known for its health benefits, in particular in inflammatory diseases.					
30818812	8	12	theme	oleic	990:994	arg1	acid					996:999	pure oleic acid	985:999	pure oleic acid	985:999	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	7	13	theme	macadamia	923:931	arg1	extracts					948:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	10	14	from	expression	1262:1271	arg1	macrophages					1316:1326	macrophages	1316:1326	macrophages	1316:1326	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	12	15	from	insights	1470:1477	arg1	mechanisms					1496:1505	the molecular mechanisms	1482:1505	the molecular mechanisms involved in the health-beneficial effects of nuts	1482:1555	We are convinced that these results provide new insights on the molecular mechanisms involved in the health-beneficial effects of nuts.					
30818812	4	16	theme	molecular	477:485	arg1	level					487:491	the molecular level	473:491	the molecular level	473:491	However, studies investigating the effects of nut extracts on inflammatory processes on the molecular level are rare.					
30818812	10	17	from	formation	1277:1285	arg1	macrophages					1316:1326	macrophages	1316:1326	macrophages	1316:1326	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	11	18	theme	beneficial	1338:1347	arg1	contribution					1349:1360	a beneficial contribution	1336:1360	a beneficial contribution of nut consumption to inflammatory diseases	1336:1404	Hence, a beneficial contribution of nut consumption to inflammatory diseases can be assumed.					
30818812	4	19	theme	extracts	435:442	arg1	effects					420:426	the effects	416:426	the effects of nut extracts on inflammatory processes on the molecular level	416:491	However, studies investigating the effects of nut extracts on inflammatory processes on the molecular level are rare.					
30818812	10	20	theme	achievable	1216:1225	arg1	concentrations					1201:1214	concentrations	1201:1214	concentrations achievable in the digestive tract	1201:1248	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	2	21	theme	fatty	261:265	arg1	composition					272:282	their beneficial fatty acid composition	244:282	their beneficial fatty acid composition	244:282	A possible mechanism for these effects could be their beneficial fatty acid composition.					
30818812	2	21	theme	fatty	261:265	arg1	mechanism					207:215	A possible mechanism	196:215	A possible mechanism for these effects	196:233	A possible mechanism for these effects could be their beneficial fatty acid composition.					
30818812	10	22	dep	expression	1262:1271	arg1	the					1258:1260	the	1258:1260	the	1258:1260	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	1	23	theme	health	139:144	arg1	benefits					146:153	its health benefits	135:153	its health benefits	135:153	Nut consumption is known for its health benefits, in particular in inflammatory diseases.					
30818812	12	24	theme	nuts	1552:1555	arg1	effects					1541:1547	the health-beneficial effects	1519:1547	the health-beneficial effects of nuts	1519:1555	We are convinced that these results provide new insights on the molecular mechanisms involved in the health-beneficial effects of nuts.					
30818812	4	25	theme	inflammatory	447:458	arg1	processes					460:468	inflammatory processes	447:468	inflammatory processes	447:468	However, studies investigating the effects of nut extracts on inflammatory processes on the molecular level are rare.					
30818812	6	26	theme	murine	700:705	arg1	RAW264.7					720:727	RAW264.7	720:727	RAW264.7	720:727	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	6	26	theme	murine	700:705	arg1	macrophages					707:717	murine macrophages	700:717	murine macrophages (RAW264.7)	700:728	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	0	27	theme	Inflammatory	68:79	arg1	Response					81:88	the Lipopolysaccharide-Induced Inflammatory Response	37:88	the Lipopolysaccharide-Induced Inflammatory Response in Macrophages	37:103	In Vitro Digested Nut Oils Attenuate the Lipopolysaccharide-Induced Inflammatory Response in Macrophages.					
30818812	5	28	theme	in	549:550	arg1	digestion					558:566	in vitro digestion	549:566	in vitro digestion	549:566	We therefore prepared oily nut extracts after in vitro digestion and saponification of the fat-soluble constituents.					
30818812	3	29	theme	fatty	331:335	arg1	acids					337:341	mono- and polyunsaturated fatty acids	305:341	mono- and polyunsaturated fatty acids	305:341	Nuts mainly contain mono- and polyunsaturated fatty acids, which have anti-inflammatory properties.					
30818812	8	30	theme	pure	985:988	arg1	acid					996:999	pure oleic acid	985:999	pure oleic acid	985:999	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	6	31	theme	cell	654:657	arg1	experiments					667:677	cell culture experiments	654:677	cell culture experiments	654:677	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	1	32	from	diseases	186:193	arg1	particular					159:168	particular	159:168	particular	159:168	Nut consumption is known for its health benefits, in particular in inflammatory diseases.					
30818812	7	33	from	acid	897:900	arg1	extracts					948:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	8	34	theme	iNos	1053:1056	arg1	expression					1039:1048	the LPS-induced expression	1023:1048	the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs	1023:1089	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	0	35	theme	In	0:1	arg1	Oils					22:25	In Vitro Digested Nut Oils	0:25	In Vitro Digested Nut Oils	0:25	In Vitro Digested Nut Oils Attenuate the Lipopolysaccharide-Induced Inflammatory Response in Macrophages.					
30818812	8	36	theme	Cox2	1059:1062	arg1	expression					1039:1048	the LPS-induced expression	1023:1048	the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs	1023:1089	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	5	37	theme	constituents	606:617	arg1	saponification					572:585	saponification	572:585	saponification	572:585	We therefore prepared oily nut extracts after in vitro digestion and saponification of the fat-soluble constituents.					
30818812	5	37	theme	constituents	606:617	arg1	digestion					558:566	in vitro digestion	549:566	in vitro digestion	549:566	We therefore prepared oily nut extracts after in vitro digestion and saponification of the fat-soluble constituents.					
30818812	1	38	theme	Nut	106:108	arg1	consumption					110:120	Nut consumption	106:120	Nut consumption	106:120	Nut consumption is known for its health benefits, in particular in inflammatory diseases.					
30818812	10	39	theme	mediators	1303:1311	arg1	formation					1277:1285	formation	1277:1285	formation	1277:1285	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	10	39	theme	mediators	1303:1311	arg1	expression					1262:1271	expression	1262:1271	expression	1262:1271	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	0	40	theme	Digested	9:16	arg1	Oils					22:25	In Vitro Digested Nut Oils	0:25	In Vitro Digested Nut Oils	0:25	In Vitro Digested Nut Oils Attenuate the Lipopolysaccharide-Induced Inflammatory Response in Macrophages.					
30818812	9	41	dep	down-regulated	1120:1133	arg1	followed					1135:1142	followed	1135:1142	followed by reduced nitric oxide formation	1135:1176	iNos protein expression was down-regulated followed by reduced nitric oxide formation.					
30818812	8	42	theme	Il1β	1071:1074	arg1	expression					1039:1048	the LPS-induced expression	1023:1048	the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs	1023:1089	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	9	43	theme	protein	1097:1103	arg1	expression					1105:1114	iNos protein expression	1092:1114	iNos protein expression	1092:1114	iNos protein expression was down-regulated followed by reduced nitric oxide formation.					
30818812	8	44	theme	mRNAs	1085:1089	arg1	expression					1039:1048	the LPS-induced expression	1023:1048	the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs	1023:1089	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	6	45	theme	inflammatory	843:854	arg1	processes					856:864	inflammatory processes	843:864	inflammatory processes	843:864	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	7	46	theme	Oleic	867:871	arg1	acid					897:900	the main fatty acid	882:900	the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts	882:955	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	7	46	theme	Oleic	867:871	arg1	acid					873:876	Oleic acid	867:876	Oleic acid	867:876	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	6	47	theme	extracts	769:776	arg1	capacity					743:750	the capacity	739:750	the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes	739:864	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	0	48	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Digested Nut Oils Attenuate the Lipopolysaccharide-Induced Inflammatory Response in Macrophages.					
30818812	8	49	theme	oily	963:966	arg1	extracts					972:979	oily nut extracts	963:979	oily nut extracts	963:979	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	11	50	theme	nut	1365:1367	arg1	consumption					1369:1379	nut consumption	1365:1379	nut consumption	1365:1379	Hence, a beneficial contribution of nut consumption to inflammatory diseases can be assumed.					
30818812	6	51	theme	different	755:763	arg1	extracts					769:776	different nut extracts	755:776	different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio)	755:829	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	9	52	theme	oxide	1162:1166	arg1	formation					1168:1176	reduced nitric oxide formation	1147:1176	reduced nitric oxide formation	1147:1176	iNos protein expression was down-regulated followed by reduced nitric oxide formation.					
30818812	7	53	theme	fatty	891:895	arg1	acid					897:900	the main fatty acid	882:900	the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts	882:955	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	7	53	theme	fatty	891:895	arg1	acid					873:876	Oleic acid	867:876	Oleic acid	867:876	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	4	54	theme	nut	431:433	arg1	extracts					435:442	nut extracts	431:442	nut extracts	431:442	However, studies investigating the effects of nut extracts on inflammatory processes on the molecular level are rare.					
30818812	10	55	from	achievable	1216:1225	arg1	tract					1244:1248	the digestive tract	1230:1248	the digestive tract	1230:1248	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	5	56	theme	nut	530:532	arg1	extracts					534:541	oily nut extracts	525:541	oily nut extracts	525:541	We therefore prepared oily nut extracts after in vitro digestion and saponification of the fat-soluble constituents.					
30818812	5	57	dep	in	549:550	arg1	vitro					552:556	vitro	552:556	vitro	552:556	We therefore prepared oily nut extracts after in vitro digestion and saponification of the fat-soluble constituents.					
30818812	6	58	theme	chromatographic	628:642	arg1	analysis					644:651	chromatographic analysis	628:651	chromatographic analysis	628:651	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	10	59	from	tract	1244:1248	arg1	achievable					1216:1225	achievable	1216:1225	achievable	1216:1225	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	10	60	theme	digestive	1234:1242	arg1	tract					1244:1248	the digestive tract	1230:1248	the digestive tract	1230:1248	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	0	61	from	Response	81:88	arg1	Macrophages					93:103	Macrophages	93:103	Macrophages	93:103	In Vitro Digested Nut Oils Attenuate the Lipopolysaccharide-Induced Inflammatory Response in Macrophages.					
30818812	3	62	theme	polyunsaturated	315:329	arg1	acids					337:341	mono- and polyunsaturated fatty acids	305:341	mono- and polyunsaturated fatty acids	305:341	Nuts mainly contain mono- and polyunsaturated fatty acids, which have anti-inflammatory properties.					
30818812	2	63	theme	acid	267:270	arg1	composition					272:282	their beneficial fatty acid composition	244:282	their beneficial fatty acid composition	244:282	A possible mechanism for these effects could be their beneficial fatty acid composition.					
30818812	2	63	theme	acid	267:270	arg1	mechanism					207:215	A possible mechanism	196:215	A possible mechanism for these effects	196:233	A possible mechanism for these effects could be their beneficial fatty acid composition.					
30818812	3	64	theme	mono-	305:309	arg1	acids					337:341	mono- and polyunsaturated fatty acids	305:341	mono- and polyunsaturated fatty acids	305:341	Nuts mainly contain mono- and polyunsaturated fatty acids, which have anti-inflammatory properties.					
30818812	2	65	theme	beneficial	250:259	arg1	composition					272:282	their beneficial fatty acid composition	244:282	their beneficial fatty acid composition	244:282	A possible mechanism for these effects could be their beneficial fatty acid composition.					
30818812	2	65	theme	beneficial	250:259	arg1	mechanism					207:215	A possible mechanism	196:215	A possible mechanism for these effects	196:233	A possible mechanism for these effects could be their beneficial fatty acid composition.					
30818812	8	66	theme	LPS-induced	1027:1037	arg1	expression					1039:1048	the LPS-induced expression	1023:1048	the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs	1023:1089	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	12	67	theme	health-beneficial	1523:1539	arg1	effects					1541:1547	the health-beneficial effects	1519:1547	the health-beneficial effects of nuts	1519:1555	We are convinced that these results provide new insights on the molecular mechanisms involved in the health-beneficial effects of nuts.					
30818812	6	68	theme	culture	659:665	arg1	experiments					667:677	cell culture experiments	654:677	cell culture experiments	654:677	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	1	69	from	particular	159:168	arg1	diseases					186:193	inflammatory diseases	173:193	inflammatory diseases	173:193	Nut consumption is known for its health benefits, in particular in inflammatory diseases.					
30818812	3	70	contain	contain	297:303	arg1	Nuts					285:288	Nuts	285:288	Nuts	285:288	Nuts mainly contain mono- and polyunsaturated fatty acids, which have anti-inflammatory properties.					
30818812	3	70	contain	contain	297:303	arg2	acids					337:341	mono- and polyunsaturated fatty acids	305:341	mono- and polyunsaturated fatty acids	305:341	Nuts mainly contain mono- and polyunsaturated fatty acids, which have anti-inflammatory properties.					
30818812	3	71	theme	anti-inflammatory	355:371	arg1	properties					373:382	anti-inflammatory properties	355:382	anti-inflammatory properties	355:382	Nuts mainly contain mono- and polyunsaturated fatty acids, which have anti-inflammatory properties.					
30818812	7	72	theme	pistachio	938:946	arg1	extracts					948:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	0	73	theme	Nut	18:20	arg1	Oils					22:25	In Vitro Digested Nut Oils	0:25	In Vitro Digested Nut Oils	0:25	In Vitro Digested Nut Oils Attenuate the Lipopolysaccharide-Induced Inflammatory Response in Macrophages.					
30818812	10	74	theme	inflammatory	1290:1301	arg1	mediators					1303:1311	inflammatory mediators	1290:1311	inflammatory mediators	1290:1311	Thus, nut extracts at concentrations achievable in the digestive tract inhibit the expression and formation of inflammatory mediators in macrophages.					
30818812	12	75	theme	molecular	1486:1494	arg1	mechanisms					1496:1505	the molecular mechanisms	1482:1505	the molecular mechanisms involved in the health-beneficial effects of nuts	1482:1555	We are convinced that these results provide new insights on the molecular mechanisms involved in the health-beneficial effects of nuts.					
30818812	9	76	theme	iNos	1092:1095	arg1	expression					1105:1114	iNos protein expression	1092:1114	iNos protein expression	1092:1114	iNos protein expression was down-regulated followed by reduced nitric oxide formation.					
30818812	0	77	theme	Lipopolysaccharide-Induced	41:66	arg1	Response					81:88	the Lipopolysaccharide-Induced Inflammatory Response	37:88	the Lipopolysaccharide-Induced Inflammatory Response in Macrophages	37:103	In Vitro Digested Nut Oils Attenuate the Lipopolysaccharide-Induced Inflammatory Response in Macrophages.					
30818812	8	78	theme	Il6	1081:1083	arg1	mRNAs					1085:1089	Il6 mRNAs	1081:1089	Il6 mRNAs	1081:1089	Both oily nut extracts and pure oleic acid significantly reduced the LPS-induced expression of iNos, Cox2, Tnfα, Il1β, and Il6 mRNAs.					
30818812	3	79	contain	have	350:353	arg2	properties					373:382	anti-inflammatory properties	355:382	anti-inflammatory properties	355:382	Nuts mainly contain mono- and polyunsaturated fatty acids, which have anti-inflammatory properties.					
30818812	3	79	contain	have	350:353	arg1	acids					337:341	mono- and polyunsaturated fatty acids	305:341	mono- and polyunsaturated fatty acids	305:341	Nuts mainly contain mono- and polyunsaturated fatty acids, which have anti-inflammatory properties.					
30818812	7	80	theme	almond	915:920	arg1	extracts					948:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	12	81	theme	new	1466:1468	arg1	insights					1470:1477	new insights	1466:1477	new insights on the molecular mechanisms involved in the health-beneficial effects of nuts	1466:1555	We are convinced that these results provide new insights on the molecular mechanisms involved in the health-beneficial effects of nuts.					
30818812	2	82	theme	possible	198:205	arg1	composition					272:282	their beneficial fatty acid composition	244:282	their beneficial fatty acid composition	244:282	A possible mechanism for these effects could be their beneficial fatty acid composition.					
30818812	2	82	theme	possible	198:205	arg1	mechanism					207:215	A possible mechanism	196:215	A possible mechanism for these effects	196:233	A possible mechanism for these effects could be their beneficial fatty acid composition.					
30818812	7	83	theme	hazelnut	905:912	arg1	extracts					948:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	hazelnut, almond, macadamia, and pistachio extracts	905:955	Oleic acid was the main fatty acid in hazelnut, almond, macadamia, and pistachio extracts.					
30818812	6	84	dep	extracts	769:776	arg1	hazelnut					779:786	hazelnut	779:786	hazelnut	779:786	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
30818812	11	85	theme	inflammatory	1384:1395	arg1	diseases					1397:1404	inflammatory diseases	1384:1404	inflammatory diseases	1384:1404	Hence, a beneficial contribution of nut consumption to inflammatory diseases can be assumed.					
30818812	6	86	theme	nut	765:767	arg1	extracts					769:776	different nut extracts	755:776	different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio)	755:829	Besides chromatographic analysis, cell culture experiments were performed using murine macrophages (RAW264.7) to study the capacity of different nut extracts (hazelnut, almond, walnut, macadamia, and pistachio) to modulate inflammatory processes.					
31744225	4	0	dep	higher	623:628	arg1	temperature					648:658	the vacuum drying temperature	630:658	The higher the vacuum drying temperature was	619:662	The higher the vacuum drying temperature was, the stronger the degradation of anthocyanins was.					
31744225	5	1	theme	better	728:733	arg1	Inulin					715:720	Inulin	715:720	Inulin	715:720	Inulin was a better protectant of anthocyanins than maltodextrin, except during vacuum drying at 90 °C, which probably triggered inulin's participation in the formation of hydroxymethyl-l-furfural (HMF), thus limiting its capability to protect anthocyanins.					
31744225	5	1	theme	better	728:733	arg1	protectant					735:744	a better protectant	726:744	a better protectant of anthocyanins	726:760	Inulin was a better protectant of anthocyanins than maltodextrin, except during vacuum drying at 90 °C, which probably triggered inulin's participation in the formation of hydroxymethyl-l-furfural (HMF), thus limiting its capability to protect anthocyanins.					
31744225	2	2	dep	-catechin	454:462	arg1	+					452:452	+	452:452	+	452:452	The highest content of identified polyphenols was noted after vacuum drying at 90 °C, which was connected with the thermally induced release of (+)-catechin.					
31744225	0	3	from	Influence	4:12	arg1	Retention					31:39	the Retention	27:39	the Retention of Polyphenolic Compounds during the Drying of Blackcurrant Juice	27:105	The Influence of Inulin on the Retention of Polyphenolic Compounds during the Drying of Blackcurrant Juice.					
31744225	6	4	theme	acids	1177:1181	arg1	content					1143:1149	the content	1139:1149	the content of flavonols and phenolic acids	1139:1181	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	1	5	theme	freeze-	149:155	arg1	methods					184:190	freeze-, vacuum-, and spray-drying methods	149:190	freeze-, vacuum-, and spray-drying methods	149:190	In blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods, 19 polyphenolic compounds were identified: anthocyanins (6), (+)-catechin, flavonols (8), and phenolic acids (4).					
31744225	5	6	from	participation	853:865	arg1	formation					874:882	the formation	870:882	the formation of hydroxymethyl-l-furfural (HMF)	870:916	Inulin was a better protectant of anthocyanins than maltodextrin, except during vacuum drying at 90 °C, which probably triggered inulin's participation in the formation of hydroxymethyl-l-furfural (HMF), thus limiting its capability to protect anthocyanins.					
31744225	6	7	theme	carrier	1101:1107	arg1	concentration					1109:1121	carrier concentration	1101:1121	carrier concentration	1101:1121	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	6	8	theme	phenolic	1168:1175	arg1	acids					1177:1181	phenolic acids	1168:1181	phenolic acids	1168:1181	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	5	9	theme	vacuum	795:800	arg1	drying					802:807	vacuum drying	795:807	vacuum drying	795:807	Inulin was a better protectant of anthocyanins than maltodextrin, except during vacuum drying at 90 °C, which probably triggered inulin's participation in the formation of hydroxymethyl-l-furfural (HMF), thus limiting its capability to protect anthocyanins.					
31744225	7	10	theme	powders	1211:1217	arg1	quality					1188:1194	The quality	1184:1194	The quality of fruit juice powders	1184:1217	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	6	11	theme	Carrier	1051:1057	arg1	selection					1059:1067	50 °C. Carrier selection	1044:1067	50 °C. Carrier selection	1044:1067	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	0	12	theme	Juice	101:105	arg1	Drying					78:83	the Drying	74:83	the Drying of Blackcurrant Juice	74:105	The Influence of Inulin on the Retention of Polyphenolic Compounds during the Drying of Blackcurrant Juice.					
31744225	1	13	theme	phenolic	287:294	arg1	4					303:303	4	303:303	4	303:303	In blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods, 19 polyphenolic compounds were identified: anthocyanins (6), (+)-catechin, flavonols (8), and phenolic acids (4).					
31744225	1	13	theme	phenolic	287:294	arg1	acids					296:300	phenolic acids	287:300	phenolic acids (4)	287:304	In blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods, 19 polyphenolic compounds were identified: anthocyanins (6), (+)-catechin, flavonols (8), and phenolic acids (4).					
31744225	6	14	theme	flavonols	1154:1162	arg1	content					1143:1149	the content	1139:1149	the content of flavonols and phenolic acids	1139:1181	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	7	15	theme	fruit	1199:1203	arg1	juice					1205:1209	fruit juice	1199:1209	fruit juice powders	1199:1217	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	7	16	theme	initial	1303:1309	arg1	composition					1311:1321	the initial composition	1299:1321	the initial composition of the material subjected to drying	1299:1357	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	6	17	from	selection	1059:1067	arg1	drying					1034:1039	vacuum drying	1027:1039	vacuum drying at 50 °C. Carrier selection affected only slightly	1027:1090	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	0	18	theme	Blackcurrant	88:99	arg1	Juice					101:105	Blackcurrant Juice	88:105	Blackcurrant Juice	88:105	The Influence of Inulin on the Retention of Polyphenolic Compounds during the Drying of Blackcurrant Juice.					
31744225	7	19	theme	material	1330:1337	arg1	composition					1311:1321	the initial composition	1299:1321	the initial composition of the material subjected to drying	1299:1357	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	7	19	theme	material	1330:1337	arg1	concentration					1404:1416	concentration	1404:1416	concentration	1404:1416	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	7	19	theme	material	1330:1337	arg1	type					1395:1398	the carrier type	1383:1398	the carrier type	1383:1398	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	7	19	theme	material	1330:1337	arg1	interactions					1427:1438	the interactions	1423:1438	the interactions that occur during the thermal treatment of fruit juices	1423:1494	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	7	19	theme	material	1330:1337	arg1	parameters					1371:1380	the drying parameters	1360:1380	the drying parameters	1360:1380	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	1	20	theme	vacuum-	158:164	arg1	methods					184:190	freeze-, vacuum-, and spray-drying methods	149:190	freeze-, vacuum-, and spray-drying methods	149:190	In blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods, 19 polyphenolic compounds were identified: anthocyanins (6), (+)-catechin, flavonols (8), and phenolic acids (4).					
31744225	4	21	theme	drying	641:646	arg1	temperature					648:658	the vacuum drying temperature	630:658	The higher the vacuum drying temperature was	619:662	The higher the vacuum drying temperature was, the stronger the degradation of anthocyanins was.					
31744225	7	22	theme	juice	1205:1209	arg1	powders					1211:1217	fruit juice powders	1199:1217	fruit juice powders	1199:1217	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	4	23	theme	vacuum	634:639	arg1	temperature					648:658	the vacuum drying temperature	630:658	The higher the vacuum drying temperature was	619:662	The higher the vacuum drying temperature was, the stronger the degradation of anthocyanins was.					
31744225	3	24	theme	reaction/caramelization	537:559	arg1	hydroxymethyl-l-furfural					570:593	hydroxymethyl-l-furfural	570:593	hydroxymethyl-l-furfural	570:593	Drying at this temperature also increased the formation of the Maillard reaction/caramelization product, hydroxymethyl-l-furfural, when inulin was added.					
31744225	3	24	theme	reaction/caramelization	537:559	arg1	product					561:567	the Maillard reaction/caramelization product	524:567	the Maillard reaction/caramelization product	524:567	Drying at this temperature also increased the formation of the Maillard reaction/caramelization product, hydroxymethyl-l-furfural, when inulin was added.					
31744225	0	25	theme	Inulin	17:22	arg1	Influence					4:12	The Influence	0:12	The Influence of Inulin on the Retention of Polyphenolic Compounds during the Drying of Blackcurrant Juice	0:105	The Influence of Inulin on the Retention of Polyphenolic Compounds during the Drying of Blackcurrant Juice.					
31744225	5	26	from	°C	815:816	arg1	drying					802:807	vacuum drying	795:807	vacuum drying	795:807	Inulin was a better protectant of anthocyanins than maltodextrin, except during vacuum drying at 90 °C, which probably triggered inulin's participation in the formation of hydroxymethyl-l-furfural (HMF), thus limiting its capability to protect anthocyanins.					
31744225	6	27	theme	50	1044:1045	arg1	°C.					1047:1049	°C.	1047:1049	°C.	1047:1049	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	7	28	theme	thermal	1462:1468	arg1	treatment					1470:1478	the thermal treatment	1458:1478	the thermal treatment of fruit juices	1458:1494	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	2	29	theme	identified	330:339	arg1	polyphenols					341:351	identified polyphenols	330:351	identified polyphenols	330:351	The highest content of identified polyphenols was noted after vacuum drying at 90 °C, which was connected with the thermally induced release of (+)-catechin.					
31744225	2	30	theme	polyphenols	341:351	arg1	content					319:325	The highest content	307:325	The highest content of identified polyphenols	307:351	The highest content of identified polyphenols was noted after vacuum drying at 90 °C, which was connected with the thermally induced release of (+)-catechin.					
31744225	3	31	theme	Maillard	528:535	arg1	hydroxymethyl-l-furfural					570:593	hydroxymethyl-l-furfural	570:593	hydroxymethyl-l-furfural	570:593	Drying at this temperature also increased the formation of the Maillard reaction/caramelization product, hydroxymethyl-l-furfural, when inulin was added.					
31744225	3	31	theme	Maillard	528:535	arg1	product					561:567	the Maillard reaction/caramelization product	524:567	the Maillard reaction/caramelization product	524:567	Drying at this temperature also increased the formation of the Maillard reaction/caramelization product, hydroxymethyl-l-furfural, when inulin was added.					
31744225	6	32	theme	phenolic	987:994	arg1	acids					996:1000	phenolic acids	987:1000	phenolic acids	987:1000	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	6	33	theme	°C.	1047:1049	arg1	selection					1059:1067	50 °C. Carrier selection	1044:1067	50 °C. Carrier selection	1044:1067	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	1	34	theme	+	255:255	arg1	-catechin					257:265	(+)-catechin	254:265	-catechin	257:265	In blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods, 19 polyphenolic compounds were identified: anthocyanins (6), (+)-catechin, flavonols (8), and phenolic acids (4).					
31744225	2	35	from	°C	389:390	arg1	drying					376:381	vacuum drying	369:381	vacuum drying	369:381	The highest content of identified polyphenols was noted after vacuum drying at 90 °C, which was connected with the thermally induced release of (+)-catechin.					
31744225	3	36	from	temperature	480:490	arg1	Drying					465:470	Drying	465:470	Drying at this temperature	465:490	Drying at this temperature also increased the formation of the Maillard reaction/caramelization product, hydroxymethyl-l-furfural, when inulin was added.					
31744225	7	37	theme	juices	1489:1494	arg1	treatment					1470:1478	the thermal treatment	1458:1478	the thermal treatment of fruit juices	1458:1494	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	2	38	theme	induced	432:438	arg1	release					440:446	the thermally induced release	418:446	the thermally induced release of (+)-catechin	418:462	The highest content of identified polyphenols was noted after vacuum drying at 90 °C, which was connected with the thermally induced release of (+)-catechin.					
31744225	2	39	theme	highest	311:317	arg1	content					319:325	The highest content	307:325	The highest content of identified polyphenols	307:351	The highest content of identified polyphenols was noted after vacuum drying at 90 °C, which was connected with the thermally induced release of (+)-catechin.					
31744225	2	40	theme	vacuum	369:374	arg1	drying					376:381	vacuum drying	369:381	vacuum drying	369:381	The highest content of identified polyphenols was noted after vacuum drying at 90 °C, which was connected with the thermally induced release of (+)-catechin.					
31744225	2	41	theme	-catechin	454:462	arg1	release					440:446	the thermally induced release	418:446	the thermally induced release of (+)-catechin	418:462	The highest content of identified polyphenols was noted after vacuum drying at 90 °C, which was connected with the thermally induced release of (+)-catechin.					
31744225	7	42	theme	carrier	1387:1393	arg1	type					1395:1398	the carrier type	1383:1398	the carrier type	1383:1398	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	7	42	theme	carrier	1387:1393	arg1	parameters					1371:1380	the drying parameters	1360:1380	the drying parameters	1360:1380	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	7	43	theme	broad	1262:1266	arg1	spectrum					1268:1275	a broad spectrum	1260:1275	a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices	1260:1494	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	6	44	theme	vacuum	1027:1032	arg1	drying					1034:1039	vacuum drying	1027:1039	vacuum drying at 50 °C. Carrier selection affected only slightly	1027:1090	Flavonols and phenolic acids were best retained after vacuum drying at 50 °C. Carrier selection affected only slightly, whereas carrier concentration did not affect, the content of flavonols and phenolic acids.					
31744225	7	45	theme	fruit	1483:1487	arg1	juices					1489:1494	fruit juices	1483:1494	fruit juices	1483:1494	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	1	46	theme	blackcurrant	111:122	arg1	powders					130:136	blackcurrant juice powders	111:136	blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods	111:190	In blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods, 19 polyphenolic compounds were identified: anthocyanins (6), (+)-catechin, flavonols (8), and phenolic acids (4).					
31744225	7	47	theme	factors	1280:1286	arg1	spectrum					1268:1275	a broad spectrum	1260:1275	a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices	1260:1494	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	1	48	theme	polyphenolic	196:207	arg1	compounds					209:217	19 polyphenolic compounds	193:217	19 polyphenolic compounds	193:217	In blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods, 19 polyphenolic compounds were identified: anthocyanins (6), (+)-catechin, flavonols (8), and phenolic acids (4).					
31744225	3	49	theme	product	561:567	arg1	formation					511:519	the formation	507:519	the formation of the Maillard reaction/caramelization product, hydroxymethyl-l-furfural,	507:594	Drying at this temperature also increased the formation of the Maillard reaction/caramelization product, hydroxymethyl-l-furfural, when inulin was added.					
31744225	5	50	theme	hydroxymethyl-l-furfural	887:910	arg1	formation					874:882	the formation	870:882	the formation of hydroxymethyl-l-furfural (HMF)	870:916	Inulin was a better protectant of anthocyanins than maltodextrin, except during vacuum drying at 90 °C, which probably triggered inulin's participation in the formation of hydroxymethyl-l-furfural (HMF), thus limiting its capability to protect anthocyanins.					
31744225	1	51	theme	juice	124:128	arg1	powders					130:136	blackcurrant juice powders	111:136	blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods	111:190	In blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods, 19 polyphenolic compounds were identified: anthocyanins (6), (+)-catechin, flavonols (8), and phenolic acids (4).					
31744225	0	52	theme	Compounds	57:65	arg1	Retention					31:39	the Retention	27:39	the Retention of Polyphenolic Compounds during the Drying of Blackcurrant Juice	27:105	The Influence of Inulin on the Retention of Polyphenolic Compounds during the Drying of Blackcurrant Juice.					
31744225	5	53	theme	anthocyanins	749:760	arg1	Inulin					715:720	Inulin	715:720	Inulin	715:720	Inulin was a better protectant of anthocyanins than maltodextrin, except during vacuum drying at 90 °C, which probably triggered inulin's participation in the formation of hydroxymethyl-l-furfural (HMF), thus limiting its capability to protect anthocyanins.					
31744225	5	53	theme	anthocyanins	749:760	arg1	protectant					735:744	a better protectant	726:744	a better protectant of anthocyanins	726:760	Inulin was a better protectant of anthocyanins than maltodextrin, except during vacuum drying at 90 °C, which probably triggered inulin's participation in the formation of hydroxymethyl-l-furfural (HMF), thus limiting its capability to protect anthocyanins.					
31744225	1	54	theme	spray-drying	171:182	arg1	methods					184:190	freeze-, vacuum-, and spray-drying methods	149:190	freeze-, vacuum-, and spray-drying methods	149:190	In blackcurrant juice powders made using freeze-, vacuum-, and spray-drying methods, 19 polyphenolic compounds were identified: anthocyanins (6), (+)-catechin, flavonols (8), and phenolic acids (4).					
31744225	0	55	theme	Polyphenolic	44:55	arg1	Compounds					57:65	Polyphenolic Compounds	44:65	Polyphenolic Compounds	44:65	The Influence of Inulin on the Retention of Polyphenolic Compounds during the Drying of Blackcurrant Juice.					
31744225	7	56	theme	drying	1364:1369	arg1	concentration					1404:1416	concentration	1404:1416	concentration	1404:1416	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	7	56	theme	drying	1364:1369	arg1	type					1395:1398	the carrier type	1383:1398	the carrier type	1383:1398	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	7	56	theme	drying	1364:1369	arg1	parameters					1371:1380	the drying parameters	1360:1380	the drying parameters	1360:1380	The quality of fruit juice powders should be considered taking into account a broad spectrum of factors, including the initial composition of the material subjected to drying, the drying parameters, the carrier type and concentration, and the interactions that occur during the thermal treatment of fruit juices.					
31744225	4	57	dep	stronger	669:676	arg1	degradation					682:692	the degradation	678:692	the stronger the degradation of anthocyanins	665:708	The higher the vacuum drying temperature was, the stronger the degradation of anthocyanins was.					
30632171	1	0	from	substance	420:428	arg1	quantification					354:367	quantification	354:367	quantification	354:367	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	1	0	from	substance	420:428	arg1	separation					339:348	separation	339:348	separation	339:348	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	2	1	theme	stationary	522:531	arg1	phase					533:537	a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase	471:537	a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase	471:537	Enantiomeric separation was achieved on a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase.					
30632171	4	2	theme	column	720:725	arg1	temperature					732:742	the column oven temperature	716:742	the column oven temperature	716:742	Isocratic elution was performed at a flow rate of 1.0 mL/min, the column oven temperature was set at 40°C and detection was at 226 nm.					
30632171	3	3	theme	Mobile	540:545	arg1	phase					547:551	Mobile phase	540:551	Mobile phase	540:551	Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v.					
30632171	1	4	theme	performance	246:256	arg1	chromatography					265:278	isocratic reverse phase high performance liquid chromatography	217:278	isocratic reverse phase high performance liquid chromatography method	217:285	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	6	5	theme	composition	889:899	arg1	impact					866:871	The impact	862:871	The impact of mobile phase composition, pH of buffer and temperature on the resolution	862:947	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	1	6	theme	liquid	258:263	arg1	chromatography					265:278	isocratic reverse phase high performance liquid chromatography	217:278	isocratic reverse phase high performance liquid chromatography method	217:285	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	6	7	from	impact	866:871	arg1	resolution					938:947	the resolution	934:947	the resolution	934:947	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	6	8	theme	phase	883:887	arg1	composition					889:899	mobile phase composition	876:899	mobile phase composition	876:899	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	6	8	theme	phase	883:887	arg1	pH					902:903	pH	902:903	pH of buffer and temperature	902:929	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	3	9	theme	hydrogen	584:591	arg1	buffer					603:608	aqueous diammonium hydrogen phosphate buffer	565:608	aqueous diammonium hydrogen phosphate buffer	565:608	Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v.					
30632171	1	10	theme	chromatography	265:278	arg1	method					280:285	isocratic reverse phase high performance liquid chromatography method	217:285	isocratic reverse phase high performance liquid chromatography method	217:285	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	6	11	theme	mobile	876:881	arg1	composition					889:899	mobile phase composition	876:899	mobile phase composition	876:899	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	6	11	theme	mobile	876:881	arg1	pH					902:903	pH	902:903	pH of buffer and temperature	902:929	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	3	12	theme	phosphate	593:601	arg1	buffer					603:608	aqueous diammonium hydrogen phosphate buffer	565:608	aqueous diammonium hydrogen phosphate buffer	565:608	Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v.					
30632171	0	13	theme	enantiomeric	112:123	arg1	purity					125:130	enantiomeric purity	112:130	enantiomeric purity of a DPP-4 inhibitor drug linagliptin	112:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	1	14	theme	S-enantiomer	372:383	arg1	quantification					354:367	quantification	354:367	quantification	354:367	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	1	14	theme	S-enantiomer	372:383	arg1	separation					339:348	separation	339:348	separation	339:348	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	5	15	theme	R	812:812	arg1	enantiomers					820:830	R and S enantiomers	812:830	R and S enantiomers	812:830	The resolution between R and S enantiomers is found to be more than 4.0.					
30632171	3	16	theme	aqueous	565:571	arg1	buffer					603:608	aqueous diammonium hydrogen phosphate buffer	565:608	aqueous diammonium hydrogen phosphate buffer	565:608	Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v.					
30632171	1	17	from	separation	339:348	arg1	substance					420:428	linagliptin (R-enantiomer) drug substance	388:428	linagliptin (R-enantiomer) drug substance	388:428	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	2	18	theme	4-chloro-3-methylphenylcarbamate	488:519	arg1	phase					533:537	a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase	471:537	a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase	471:537	Enantiomeric separation was achieved on a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase.					
30632171	9	19	theme	S-enantiomer	1162:1173	arg1	recovery					1150:1157	The recovery	1146:1157	The recovery of S-enantiomer	1146:1173	The recovery of S-enantiomer is 99.8% w/w.					
30632171	9	19	theme	S-enantiomer	1162:1173	arg1	%					1182:1182	99.8% w/w	1178:1186	99.8% w/w	1178:1186	The recovery of S-enantiomer is 99.8% w/w.					
30632171	3	20	theme	diammonium	573:582	arg1	buffer					603:608	aqueous diammonium hydrogen phosphate buffer	565:608	aqueous diammonium hydrogen phosphate buffer	565:608	Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v.					
30632171	10	21	theme	proposed	1193:1200	arg1	method					1202:1207	The proposed method	1189:1207	The proposed method	1189:1207	The proposed method is validated for specificity, precision, linearity, accuracy and robustness.					
30632171	4	22	theme	flow	691:694	arg1	rate					696:699	a flow rate	689:699	a flow rate of 1.0 mL/min	689:713	Isocratic elution was performed at a flow rate of 1.0 mL/min, the column oven temperature was set at 40°C and detection was at 226 nm.					
30632171	0	23	theme	new	2:4	arg1	stability					6:14	A new stability	0:14	A new stability	0:14	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	1	24	theme	linagliptin	388:398	arg1	substance					420:428	linagliptin (R-enantiomer) drug substance	388:428	linagliptin (R-enantiomer) drug substance	388:428	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	7	25	theme	0.17-1.7 μg/mL	1043:1056	arg1	range					1034:1038	the concentration range	1016:1038	the concentration range of 0.17-1.7 μg/mL	1016:1056	The detector response is found to be linear over the concentration range of 0.17-1.7 μg/mL.					
30632171	8	26	theme	LOQ	1067:1069	arg1	levels					1071:1076	LOD and LOQ levels	1059:1076	LOD and LOQ levels of S-enantiomer	1059:1092	LOD and LOQ levels of S-enantiomer are found to be 0.057 and 0.172 μg/mL respectively.					
30632171	5	27	theme	S	818:818	arg1	enantiomers					820:830	R and S enantiomers	812:830	R and S enantiomers	812:830	The resolution between R and S enantiomers is found to be more than 4.0.					
30632171	1	28	from	S-enantiomer	372:383	arg1	substance					420:428	linagliptin (R-enantiomer) drug substance	388:428	linagliptin (R-enantiomer) drug substance	388:428	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	0	29	theme	purity	125:130	arg1	determination					95:107	the determination	91:107	the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin	91:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	2	30	theme	tris	483:486	arg1	phase					533:537	a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase	471:537	a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase	471:537	Enantiomeric separation was achieved on a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase.					
30632171	2	31	theme	Enantiomeric	431:442	arg1	separation					444:453	Enantiomeric separation	431:453	Enantiomeric separation	431:453	Enantiomeric separation was achieved on a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase.					
30632171	0	32	theme	phase	35:39	arg1	chromatography					65:78	reverse phase high performance liquid chromatography	27:78	reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin	27:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	1	33	dep	separation	339:348	arg1	the					335:337	the	335:337	the	335:337	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	0	34	theme	DPP-4	137:141	arg1	linagliptin					158:168	a DPP-4 inhibitor drug linagliptin	135:168	a DPP-4 inhibitor drug linagliptin	135:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	1	35	theme	R-enantiomer	401:412	arg1	substance					420:428	linagliptin (R-enantiomer) drug substance	388:428	linagliptin (R-enantiomer) drug substance	388:428	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	0	36	theme	reverse	27:33	arg1	chromatography					65:78	reverse phase high performance liquid chromatography	27:78	reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin	27:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	6	37	theme	temperature	919:929	arg1	composition					889:899	mobile phase composition	876:899	mobile phase composition	876:899	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	6	37	theme	temperature	919:929	arg1	pH					902:903	pH	902:903	pH of buffer and temperature	902:929	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	1	38	from	quantification	354:367	arg1	substance					420:428	linagliptin (R-enantiomer) drug substance	388:428	linagliptin (R-enantiomer) drug substance	388:428	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	3	39	theme	35:65	643:647	arg1	v/v					649:651	35:65 v/v	643:651	35:65 v/v	643:651	Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v.					
30632171	0	40	theme	performance	46:56	arg1	chromatography					65:78	reverse phase high performance liquid chromatography	27:78	reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin	27:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	0	41	theme	drug	153:156	arg1	linagliptin					158:168	a DPP-4 inhibitor drug linagliptin	135:168	a DPP-4 inhibitor drug linagliptin	135:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	1	42	theme	drug	415:418	arg1	substance					420:428	linagliptin (R-enantiomer) drug substance	388:428	linagliptin (R-enantiomer) drug substance	388:428	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	7	43	theme	concentration	1020:1032	arg1	range					1034:1038	the concentration range	1016:1038	the concentration range of 0.17-1.7 μg/mL	1016:1056	The detector response is found to be linear over the concentration range of 0.17-1.7 μg/mL.					
30632171	0	44	theme	high	41:44	arg1	chromatography					65:78	reverse phase high performance liquid chromatography	27:78	reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin	27:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	6	45	theme	buffer	908:913	arg1	composition					889:899	mobile phase composition	876:899	mobile phase composition	876:899	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	6	45	theme	buffer	908:913	arg1	pH					902:903	pH	902:903	pH of buffer and temperature	902:929	The impact of mobile phase composition, pH of buffer and temperature on the resolution has been studied.					
30632171	0	46	theme	inhibitor	143:151	arg1	linagliptin					158:168	a DPP-4 inhibitor drug linagliptin	135:168	a DPP-4 inhibitor drug linagliptin	135:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	2	47	theme	Cellulose	473:481	arg1	phase					533:537	a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase	471:537	a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase	471:537	Enantiomeric separation was achieved on a Cellulose tris(4-chloro-3-methylphenylcarbamate) stationary phase.					
30632171	7	48	theme	detector	971:978	arg1	linear					1004:1009	linear	1004:1009	linear	1004:1009	The detector response is found to be linear over the concentration range of 0.17-1.7 μg/mL.					
30632171	7	48	theme	detector	971:978	arg1	response					980:987	The detector response	967:987	The detector response	967:987	The detector response is found to be linear over the concentration range of 0.17-1.7 μg/mL.					
30632171	4	49	theme	oven	727:730	arg1	temperature					732:742	the column oven temperature	716:742	the column oven temperature	716:742	Isocratic elution was performed at a flow rate of 1.0 mL/min, the column oven temperature was set at 40°C and detection was at 226 nm.					
30632171	3	50	from	buffer	603:608	arg1	ratio					634:638	the ratio	630:638	the ratio of 35:65 v/v	630:651	Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v.					
30632171	8	51	theme	S-enantiomer	1081:1092	arg1	levels					1071:1076	LOD and LOQ levels	1059:1076	LOD and LOQ levels of S-enantiomer	1059:1092	LOD and LOQ levels of S-enantiomer are found to be 0.057 and 0.172 μg/mL respectively.					
30632171	1	52	theme	isocratic	217:225	arg1	chromatography					265:278	isocratic reverse phase high performance liquid chromatography	217:278	isocratic reverse phase high performance liquid chromatography method	217:285	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	0	53	theme	linagliptin	158:168	arg1	purity					125:130	enantiomeric purity	112:130	enantiomeric purity of a DPP-4 inhibitor drug linagliptin	112:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	3	54	from	acetonitrile	614:625	arg1	ratio					634:638	the ratio	630:638	the ratio of 35:65 v/v	630:651	Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v.					
30632171	1	55	theme	reverse	227:233	arg1	chromatography					265:278	isocratic reverse phase high performance liquid chromatography	217:278	isocratic reverse phase high performance liquid chromatography method	217:285	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	0	56	theme	chromatography	65:78	arg1	method					80:85	reverse phase high performance liquid chromatography method	27:85	reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin	27:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	3	57	theme	v/v	649:651	arg1	ratio					634:638	the ratio	630:638	the ratio of 35:65 v/v	630:651	Mobile phase consists of aqueous diammonium hydrogen phosphate buffer and acetonitrile in the ratio of 35:65 v/v.					
30632171	1	58	theme	phase	235:239	arg1	chromatography					265:278	isocratic reverse phase high performance liquid chromatography	217:278	isocratic reverse phase high performance liquid chromatography method	217:285	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
30632171	0	59	theme	liquid	58:63	arg1	chromatography					65:78	reverse phase high performance liquid chromatography	27:78	reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin	27:168	A new stability indicating reverse phase high performance liquid chromatography method for the determination of enantiomeric purity of a DPP-4 inhibitor drug linagliptin.					
30632171	4	60	theme	Isocratic	654:662	arg1	elution					664:670	Isocratic elution	654:670	Isocratic elution	654:670	Isocratic elution was performed at a flow rate of 1.0 mL/min, the column oven temperature was set at 40°C and detection was at 226 nm.					
30632171	4	61	theme	mL/min	708:713	arg1	rate					696:699	a flow rate	689:699	a flow rate of 1.0 mL/min	689:713	Isocratic elution was performed at a flow rate of 1.0 mL/min, the column oven temperature was set at 40°C and detection was at 226 nm.					
30632171	8	62	theme	LOD	1059:1061	arg1	levels					1071:1076	LOD and LOQ levels	1059:1076	LOD and LOQ levels of S-enantiomer	1059:1092	LOD and LOQ levels of S-enantiomer are found to be 0.057 and 0.172 μg/mL respectively.					
30632171	1	63	theme	high	241:244	arg1	chromatography					265:278	isocratic reverse phase high performance liquid chromatography	217:278	isocratic reverse phase high performance liquid chromatography method	217:285	A simple, sensitive, and stability indicating isocratic reverse phase high performance liquid chromatography method has been developed, optimized and validated for the separation and quantification of S-enantiomer in linagliptin (R-enantiomer) drug substance.					
29562782	3	0	contain	have	537:540	arg1	constructs					521:530	hydrogel-based constructs	506:530	hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage	506:614	Great promise for osteochondral regeneration is held in hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage.					
29562782	3	0	contain	have	537:540	arg2	composition					553:563	a flexible composition	542:563	a flexible composition	542:563	Great promise for osteochondral regeneration is held in hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage.					
29562782	2	1	theme	clinical	373:380	arg1	problem					382:388	a major clinical problem	365:388	a major clinical problem with lifelong chronic pain and compromised quality of life	365:447	Osteochondral defects represent a major clinical problem with lifelong chronic pain and compromised quality of life.					
29562782	1	2	theme	placenta	237:244	arg1	membrane					162:169	the human amniotic membrane	143:169	the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta	143:244	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	6	3	theme	hAMSCs	932:937	arg1	Immobilization					914:927	Immobilization	914:927	Immobilization of hAMSCs into pectin gels	914:954	Immobilization of hAMSCs into pectin gels could expand their application in cell-based bioengineering strategies.					
29562782	5	4	theme	3D	814:815	arg1	scaffolds					817:825	3D scaffolds	814:825	3D scaffolds	814:825	As pectin gels have also been explored as cell vehicles on 3D scaffolds, the aim of this study was to explore the possibility to include hAMSCs in pectin gel.					
29562782	7	5	theme	osteogenic	1152:1161	arg1	lineage					1163:1169	the osteogenic lineage	1148:1169	the osteogenic lineage	1148:1169	hAMSCs were analyzed for their viability and recovery from the pectin gel and for their ability to differentiate toward the osteogenic lineage and to maintain their immunological characteristics.					
29562782	1	6	from	membrane	162:169	arg1	cells					132:136	Mesenchymal stromal cells	112:136	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta	112:244	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	2	7	theme	major	367:371	arg1	problem					382:388	a major clinical problem	365:388	a major clinical problem with lifelong chronic pain and compromised quality of life	365:447	Osteochondral defects represent a major clinical problem with lifelong chronic pain and compromised quality of life.					
29562782	4	8	theme	Cell	617:620	arg1	loading					622:628	Cell loading	617:628	Cell loading within a hydrogel	617:646	Cell loading within a hydrogel represents an advantage for regenerative purposes, but the encapsulation steps can modify cell properties.					
29562782	3	9	theme	flexible	544:551	arg1	composition					553:563	a flexible composition	542:563	a flexible composition	542:563	Great promise for osteochondral regeneration is held in hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage.					
29562782	5	10	theme	pectin	902:907	arg1	gel					909:911	pectin gel	902:911	pectin gel	902:911	As pectin gels have also been explored as cell vehicles on 3D scaffolds, the aim of this study was to explore the possibility to include hAMSCs in pectin gel.					
29562782	9	11	used	used	1534:1537	arg2	hAMSCs					1518:1523	hAMSCs	1518:1523	hAMSCs	1518:1523	Taken together, these results suggest that hAMSCs could be used in combination to pectin gels for the study of novel osteochondral regeneration strategies.					
29562782	7	12	from	gel	1098:1100	arg1	viability					1059:1067	viability	1059:1067	viability	1059:1067	hAMSCs were analyzed for their viability and recovery from the pectin gel and for their ability to differentiate toward the osteogenic lineage and to maintain their immunological characteristics.					
29562782	0	13	theme	Pectin	100:105	arg1	Gel					107:109	Pectin Gel	100:109	Pectin Gel	100:109	Immunological and Differentiation Properties of Amniotic Cells Are Retained After Immobilization in Pectin Gel.					
29562782	3	14	theme	physiological	579:591	arg1	structure					593:601	the physiological structure	575:601	the physiological structure of cartilage	575:614	Great promise for osteochondral regeneration is held in hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage.					
29562782	3	15	theme	hydrogel-based	506:519	arg1	constructs					521:530	hydrogel-based constructs	506:530	hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage	506:614	Great promise for osteochondral regeneration is held in hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage.					
29562782	4	16	theme	cell	738:741	arg1	properties					743:752	cell properties	738:752	cell properties	738:752	Cell loading within a hydrogel represents an advantage for regenerative purposes, but the encapsulation steps can modify cell properties.					
29562782	7	17	theme	immunological	1193:1205	arg1	characteristics					1207:1221	their immunological characteristics	1187:1221	their immunological characteristics	1187:1221	hAMSCs were analyzed for their viability and recovery from the pectin gel and for their ability to differentiate toward the osteogenic lineage and to maintain their immunological characteristics.					
29562782	9	18	theme	novel	1586:1590	arg1	strategies					1619:1628	novel osteochondral regeneration strategies	1586:1628	novel osteochondral regeneration strategies	1586:1628	Taken together, these results suggest that hAMSCs could be used in combination to pectin gels for the study of novel osteochondral regeneration strategies.					
29562782	2	19	theme	chronic	404:410	arg1	pain					412:415	lifelong chronic pain	395:415	lifelong chronic pain	395:415	Osteochondral defects represent a major clinical problem with lifelong chronic pain and compromised quality of life.					
29562782	6	20	theme	bioengineering	1001:1014	arg1	strategies					1016:1025	cell-based bioengineering strategies	990:1025	cell-based bioengineering strategies	990:1025	Immobilization of hAMSCs into pectin gels could expand their application in cell-based bioengineering strategies.					
29562782	1	21	from	applications	294:305	arg1	medicine					323:330	regenerative medicine	310:330	regenerative medicine	310:330	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	4	22	theme	regenerative	676:687	arg1	purposes					689:696	regenerative purposes	676:696	regenerative purposes	676:696	Cell loading within a hydrogel represents an advantage for regenerative purposes, but the encapsulation steps can modify cell properties.					
29562782	9	23	theme	osteochondral	1592:1604	arg1	strategies					1619:1628	novel osteochondral regeneration strategies	1586:1628	novel osteochondral regeneration strategies	1586:1628	Taken together, these results suggest that hAMSCs could be used in combination to pectin gels for the study of novel osteochondral regeneration strategies.					
29562782	1	24	dep	membrane	162:169	arg1	cells					213:217	human amniotic mesenchymal stromal cells	178:217	human amniotic mesenchymal stromal cells [hAMSCs]	178:226	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	1	25	theme	human	178:182	arg1	cells					213:217	human amniotic mesenchymal stromal cells	178:217	human amniotic mesenchymal stromal cells [hAMSCs]	178:226	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	6	26	theme	cell-based	990:999	arg1	strategies					1016:1025	cell-based bioengineering strategies	990:1025	cell-based bioengineering strategies	990:1025	Immobilization of hAMSCs into pectin gels could expand their application in cell-based bioengineering strategies.					
29562782	6	27	theme	pectin	944:949	arg1	gels					951:954	pectin gels	944:954	pectin gels	944:954	Immobilization of hAMSCs into pectin gels could expand their application in cell-based bioengineering strategies.					
29562782	9	28	theme	pectin	1557:1562	arg1	gels					1564:1567	pectin gels	1557:1567	pectin gels	1557:1567	Taken together, these results suggest that hAMSCs could be used in combination to pectin gels for the study of novel osteochondral regeneration strategies.					
29562782	1	29	theme	amniotic	184:191	arg1	cells					213:217	human amniotic mesenchymal stromal cells	178:217	human amniotic mesenchymal stromal cells [hAMSCs]	178:226	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	8	30	theme	pectin/hydroxyapatite	1263:1283	arg1	biocomposite					1289:1300	a purposely designed pectin/hydroxyapatite gel biocomposite	1242:1300	a purposely designed pectin/hydroxyapatite gel biocomposite	1242:1300	When treated with a purposely designed pectin/hydroxyapatite gel biocomposite, hAMSCs retained their ability to differentiate toward the osteogenic lineage, did not induce an immune response, and retained their ability to reduce T cell proliferation.					
29562782	6	31	from	application	975:985	arg1	strategies					1016:1025	cell-based bioengineering strategies	990:1025	cell-based bioengineering strategies	990:1025	Immobilization of hAMSCs into pectin gels could expand their application in cell-based bioengineering strategies.					
29562782	8	32	theme	designed	1254:1261	arg1	biocomposite					1289:1300	a purposely designed pectin/hydroxyapatite gel biocomposite	1242:1300	a purposely designed pectin/hydroxyapatite gel biocomposite	1242:1300	When treated with a purposely designed pectin/hydroxyapatite gel biocomposite, hAMSCs retained their ability to differentiate toward the osteogenic lineage, did not induce an immune response, and retained their ability to reduce T cell proliferation.					
29562782	2	33	theme	life	444:447	arg1	pain					412:415	lifelong chronic pain	395:415	lifelong chronic pain	395:415	Osteochondral defects represent a major clinical problem with lifelong chronic pain and compromised quality of life.					
29562782	2	33	theme	life	444:447	arg1	quality					433:439	compromised quality	421:439	compromised quality of life	421:447	Osteochondral defects represent a major clinical problem with lifelong chronic pain and compromised quality of life.					
29562782	5	34	theme	study	844:848	arg1	aim					832:834	the aim	828:834	the aim of this study	828:848	As pectin gels have also been explored as cell vehicles on 3D scaffolds, the aim of this study was to explore the possibility to include hAMSCs in pectin gel.					
29562782	1	35	theme	Mesenchymal	112:122	arg1	cells					132:136	Mesenchymal stromal cells	112:136	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta	112:244	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	1	36	theme	stromal	205:211	arg1	cells					213:217	human amniotic mesenchymal stromal cells	178:217	human amniotic mesenchymal stromal cells [hAMSCs]	178:226	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	0	37	theme	Amniotic	48:55	arg1	Cells					57:61	Amniotic Cells	48:61	Amniotic Cells	48:61	Immunological and Differentiation Properties of Amniotic Cells Are Retained After Immobilization in Pectin Gel.					
29562782	9	38	theme	regeneration	1606:1617	arg1	strategies					1619:1628	novel osteochondral regeneration strategies	1586:1628	novel osteochondral regeneration strategies	1586:1628	Taken together, these results suggest that hAMSCs could be used in combination to pectin gels for the study of novel osteochondral regeneration strategies.					
29562782	5	39	theme	pectin	758:763	arg1	vehicles					802:809	cell vehicles	797:809	cell vehicles on 3D scaffolds	797:825	As pectin gels have also been explored as cell vehicles on 3D scaffolds, the aim of this study was to explore the possibility to include hAMSCs in pectin gel.					
29562782	5	39	theme	pectin	758:763	arg1	gels					765:768	pectin gels	758:768	pectin gels	758:768	As pectin gels have also been explored as cell vehicles on 3D scaffolds, the aim of this study was to explore the possibility to include hAMSCs in pectin gel.					
29562782	3	40	theme	Great	450:454	arg1	promise					456:462	Great promise	450:462	Great promise for osteochondral regeneration	450:493	Great promise for osteochondral regeneration is held in hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage.					
29562782	3	41	theme	osteochondral	468:480	arg1	regeneration					482:493	osteochondral regeneration	468:493	osteochondral regeneration	468:493	Great promise for osteochondral regeneration is held in hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage.					
29562782	7	42	theme	pectin	1091:1096	arg1	gel					1098:1100	the pectin gel	1087:1100	the pectin gel	1087:1100	hAMSCs were analyzed for their viability and recovery from the pectin gel and for their ability to differentiate toward the osteogenic lineage and to maintain their immunological characteristics.					
29562782	2	43	theme	compromised	421:431	arg1	quality					433:439	compromised quality	421:439	compromised quality of life	421:447	Osteochondral defects represent a major clinical problem with lifelong chronic pain and compromised quality of life.					
29562782	7	44	dep	ability	1116:1122	arg1	maintain					1178:1185	maintain	1178:1185	to maintain their immunological characteristics	1175:1221	hAMSCs were analyzed for their viability and recovery from the pectin gel and for their ability to differentiate toward the osteogenic lineage and to maintain their immunological characteristics.					
29562782	7	44	dep	ability	1116:1122	arg1	differentiate					1127:1139	differentiate	1127:1139	to differentiate toward the osteogenic lineage	1124:1169	hAMSCs were analyzed for their viability and recovery from the pectin gel and for their ability to differentiate toward the osteogenic lineage and to maintain their immunological characteristics.					
29562782	1	45	theme	regenerative	310:321	arg1	medicine					323:330	regenerative medicine	310:330	regenerative medicine	310:330	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	5	46	from	hAMSCs	892:897	arg1	gel					909:911	pectin gel	902:911	pectin gel	902:911	As pectin gels have also been explored as cell vehicles on 3D scaffolds, the aim of this study was to explore the possibility to include hAMSCs in pectin gel.					
29562782	8	47	theme	cell	1455:1458	arg1	proliferation					1460:1472	T cell proliferation	1453:1472	T cell proliferation	1453:1472	When treated with a purposely designed pectin/hydroxyapatite gel biocomposite, hAMSCs retained their ability to differentiate toward the osteogenic lineage, did not induce an immune response, and retained their ability to reduce T cell proliferation.					
29562782	1	48	theme	stromal	124:130	arg1	cells					132:136	Mesenchymal stromal cells	112:136	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta	112:244	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	0	49	theme	Cells	57:61	arg1	Immunological					0:12	Immunological	0:12	Immunological	0:12	Immunological and Differentiation Properties of Amniotic Cells Are Retained After Immobilization in Pectin Gel.					
29562782	5	50	from	vehicles	802:809	arg1	scaffolds					817:825	3D scaffolds	814:825	3D scaffolds	814:825	As pectin gels have also been explored as cell vehicles on 3D scaffolds, the aim of this study was to explore the possibility to include hAMSCs in pectin gel.					
29562782	8	51	theme	T	1453:1453	arg1	proliferation					1460:1472	T cell proliferation	1453:1472	T cell proliferation	1453:1472	When treated with a purposely designed pectin/hydroxyapatite gel biocomposite, hAMSCs retained their ability to differentiate toward the osteogenic lineage, did not induce an immune response, and retained their ability to reduce T cell proliferation.					
29562782	4	52	theme	encapsulation	707:719	arg1	steps					721:725	the encapsulation steps	703:725	the encapsulation steps	703:725	Cell loading within a hydrogel represents an advantage for regenerative purposes, but the encapsulation steps can modify cell properties.					
29562782	3	53	theme	cartilage	606:614	arg1	structure					593:601	the physiological structure	575:601	the physiological structure of cartilage	575:614	Great promise for osteochondral regeneration is held in hydrogel-based constructs that have a flexible composition and mimic the physiological structure of cartilage.					
29562782	1	54	dep	cells	213:217	arg1	i.e.					172:175	i.e.	172:175	i.e.	172:175	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	1	54	dep	cells	213:217	arg1	[hAMSCs					219:225	[hAMSCs	219:225	[hAMSCs	219:225	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	9	55	theme	strategies	1619:1628	arg1	study					1577:1581	the study	1573:1581	the study of novel osteochondral regeneration strategies	1573:1628	Taken together, these results suggest that hAMSCs could be used in combination to pectin gels for the study of novel osteochondral regeneration strategies.					
29562782	8	56	theme	immune	1399:1404	arg1	response					1406:1413	an immune response	1396:1413	an immune response	1396:1413	When treated with a purposely designed pectin/hydroxyapatite gel biocomposite, hAMSCs retained their ability to differentiate toward the osteogenic lineage, did not induce an immune response, and retained their ability to reduce T cell proliferation.					
29562782	2	57	theme	Osteochondral	333:345	arg1	defects					347:353	Osteochondral defects	333:353	Osteochondral defects	333:353	Osteochondral defects represent a major clinical problem with lifelong chronic pain and compromised quality of life.					
29562782	8	58	theme	gel	1285:1287	arg1	biocomposite					1289:1300	a purposely designed pectin/hydroxyapatite gel biocomposite	1242:1300	a purposely designed pectin/hydroxyapatite gel biocomposite	1242:1300	When treated with a purposely designed pectin/hydroxyapatite gel biocomposite, hAMSCs retained their ability to differentiate toward the osteogenic lineage, did not induce an immune response, and retained their ability to reduce T cell proliferation.					
29562782	2	59	with	problem	382:388	arg1	pain					412:415	lifelong chronic pain	395:415	lifelong chronic pain	395:415	Osteochondral defects represent a major clinical problem with lifelong chronic pain and compromised quality of life.					
29562782	2	59	with	problem	382:388	arg1	quality					433:439	compromised quality	421:439	compromised quality of life	421:447	Osteochondral defects represent a major clinical problem with lifelong chronic pain and compromised quality of life.					
29562782	1	60	theme	mesenchymal	193:203	arg1	cells					213:217	human amniotic mesenchymal stromal cells	178:217	human amniotic mesenchymal stromal cells [hAMSCs]	178:226	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	1	61	theme	human	147:151	arg1	membrane					162:169	the human amniotic membrane	143:169	the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta	143:244	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	8	62	theme	osteogenic	1361:1370	arg1	lineage					1372:1378	the osteogenic lineage	1357:1378	the osteogenic lineage	1357:1378	When treated with a purposely designed pectin/hydroxyapatite gel biocomposite, hAMSCs retained their ability to differentiate toward the osteogenic lineage, did not induce an immune response, and retained their ability to reduce T cell proliferation.					
29562782	5	63	theme	cell	797:800	arg1	vehicles					802:809	cell vehicles	797:809	cell vehicles on 3D scaffolds	797:825	As pectin gels have also been explored as cell vehicles on 3D scaffolds, the aim of this study was to explore the possibility to include hAMSCs in pectin gel.					
29562782	5	63	theme	cell	797:800	arg1	gels					765:768	pectin gels	758:768	pectin gels	758:768	As pectin gels have also been explored as cell vehicles on 3D scaffolds, the aim of this study was to explore the possibility to include hAMSCs in pectin gel.					
29562782	0	64	dep	Immunological	0:12	arg1	Properties					34:43	Properties	34:43	Properties	34:43	Immunological and Differentiation Properties of Amniotic Cells Are Retained After Immobilization in Pectin Gel.					
29562782	1	65	theme	amniotic	153:160	arg1	membrane					162:169	the human amniotic membrane	143:169	the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta	143:244	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29562782	1	66	theme	term	232:235	arg1	placenta					237:244	term placenta	232:244	term placenta	232:244	Mesenchymal stromal cells from the human amniotic membrane (i.e., human amniotic mesenchymal stromal cells [hAMSCs]) of term placenta are increasingly attracting attention for their applications in regenerative medicine.					
29925435	0	0	theme	tumor	83:87	arg1	profiles					99:106	tumor CD24/CD44 profiles	83:106	tumor CD24/CD44 profiles	83:106	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	7	1	theme	cancer	1166:1171	arg1	cells					1178:1182	triple-negative cancer stem cells	1150:1182	triple-negative cancer stem cells	1150:1182	Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences.					
29925435	2	2	theme	tumor	365:369	arg1	samples					371:377	fresh and freeze/thawed dissociated tumor samples	329:377	fresh and freeze/thawed dissociated tumor samples	329:377	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	6	3	theme	frozen	1028:1033	arg1	samples					1035:1041	fresh and frozen samples	1018:1041	fresh and frozen samples from the same patient	1018:1063	These cryopreservation-driven alterations in cellular phenotype make it impossible to compare fresh and frozen samples from the same patient directly.					
29925435	8	4	theme	tumor	1361:1365	arg1	cells					1367:1371	living tumor cells	1354:1371	living tumor cells	1354:1371	Overall, this study suggests that all analyses of living tumor cells should be conducted only using freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers.					
29925435	7	5	theme	stem	1173:1176	arg1	cells					1178:1182	triple-negative cancer stem cells	1150:1182	triple-negative cancer stem cells	1150:1182	Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences.					
29925435	5	6	theme	tumor-infiltrating	891:908	arg1	cells					917:921	tumor-infiltrating immune cells	891:921	tumor-infiltrating immune cells	891:921	This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells.					
29925435	2	7	theme	dissociated	353:363	arg1	samples					371:377	fresh and freeze/thawed dissociated tumor samples	329:377	fresh and freeze/thawed dissociated tumor samples	329:377	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	6	8	theme	fresh	1018:1022	arg1	samples					1035:1041	fresh and frozen samples	1018:1041	fresh and frozen samples from the same patient	1018:1063	These cryopreservation-driven alterations in cellular phenotype make it impossible to compare fresh and frozen samples from the same patient directly.					
29925435	0	9	theme	CD24/CD44	89:97	arg1	profiles					99:106	tumor CD24/CD44 profiles	83:106	tumor CD24/CD44 profiles	83:106	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	7	10	theme	triple-negative	1150:1164	arg1	cells					1178:1182	triple-negative cancer stem cells	1150:1182	triple-negative cancer stem cells	1150:1182	Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences.					
29925435	4	11	theme	marked	698:703	arg1	reduction					705:713	a marked reduction	696:713	a marked reduction in the number of CD45-CD44LowCD24Low tumor cells	696:762	We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells.					
29925435	3	12	theme	luminal	508:514	arg1	A					516:516	luminal A	508:516	luminal A	508:516	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	7	13	theme	transcriptomic	1121:1134	arg1	signatures					1136:1145	the transcriptomic signatures	1117:1145	the transcriptomic signatures of triple-negative cancer stem cells	1117:1182	Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences.					
29925435	5	14	theme	certain	823:829	arg1	patterns					852:859	certain CD44/CD24 expression patterns	823:859	certain CD44/CD24 expression patterns	823:859	This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells.					
29925435	3	15	theme	breast	547:552	arg1	TNBC					562:565	TNBC	562:565	TNBC	562:565	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	3	15	theme	breast	547:552	arg1	cancer					554:559	basal/triple-negative breast cancer	525:559	basal/triple-negative breast cancer (TNBC)	525:566	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	3	16	theme	Breast	453:458	arg1	cancer					460:465	RESULTS Breast cancer	445:465	RESULTS Breast cancer	445:465	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	3	16	theme	Breast	453:458	arg1	disease					486:492	a heterogeneous disease	470:492	a heterogeneous disease	470:492	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	7	17	theme	cells	1178:1182	arg1	signatures					1136:1145	the transcriptomic signatures	1117:1145	the transcriptomic signatures of triple-negative cancer stem cells	1117:1182	Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences.					
29925435	5	18	theme	immune	910:915	arg1	cells					917:921	tumor-infiltrating immune cells	891:921	tumor-infiltrating immune cells	891:921	This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells.					
29925435	7	19	theme	hierarchical	1206:1217	arg1	clustering					1219:1228	hierarchical clustering	1206:1228	hierarchical clustering	1206:1228	Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences.					
29925435	6	20	from	patient	1057:1063	arg1	samples					1035:1041	fresh and frozen samples	1018:1041	fresh and frozen samples from the same patient	1018:1063	These cryopreservation-driven alterations in cellular phenotype make it impossible to compare fresh and frozen samples from the same patient directly.					
29925435	4	21	theme	CD45-CD44LowCD24Low	732:750	arg1	cells					758:762	CD45-CD44LowCD24Low tumor cells	732:762	CD45-CD44LowCD24Low tumor cells	732:762	We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells.					
29925435	6	22	from	alterations	954:964	arg1	phenotype					978:986	cellular phenotype	969:986	cellular phenotype	969:986	These cryopreservation-driven alterations in cellular phenotype make it impossible to compare fresh and frozen samples from the same patient directly.					
29925435	1	23	theme	novel	184:188	arg1	methods					190:196	novel methods	184:196	novel methods	184:196	OBJECTIVE The use of novel methods to characterize living tumor cells relies on well-conceived biobanks.					
29925435	0	24	theme	Tumor	0:4	arg1	analysis					6:13	Tumor analysis	0:13	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells	0:74	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	3	25	theme	ERBB2-like	573:582	arg1	tumors					584:589	ERBB2-like tumors	573:589	ERBB2-like tumors	573:589	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	1	26	theme	methods	190:196	arg1	use					177:179	The use	173:179	The use of novel methods	173:196	OBJECTIVE The use of novel methods to characterize living tumor cells relies on well-conceived biobanks.					
29925435	5	27	theme	tumor	806:810	arg1	cells					812:816	tumor cells	806:816	tumor cells	806:816	This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells.					
29925435	8	28	theme	living	1354:1359	arg1	cells					1367:1371	living tumor cells	1354:1371	living tumor cells	1354:1371	Overall, this study suggests that all analyses of living tumor cells should be conducted only using freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers.					
29925435	7	29	theme	freeze/thaw	1089:1099	arg1	process					1101:1107	the freeze/thaw process	1085:1107	the freeze/thaw process	1085:1107	Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences.					
29925435	4	30	theme	cells	758:762	arg1	number					722:727	the number	718:727	the number of CD45-CD44LowCD24Low tumor cells	718:762	We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells.					
29925435	2	31	theme	profiling	434:442	arg1	terms					396:400	terms	396:400	terms of quantitative and qualitative profiling	396:442	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	5	32	theme	cells	812:816	arg1	percentage					792:801	the percentage	788:801	the percentage of tumor cells with certain CD44/CD24 expression patterns	788:859	This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells.					
29925435	4	33	theme	tumor	752:756	arg1	cells					758:762	CD45-CD44LowCD24Low tumor cells	732:762	CD45-CD44LowCD24Low tumor cells	732:762	We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells.					
29925435	2	34	theme	qualitative	422:432	arg1	profiling					434:442	quantitative and qualitative profiling	405:442	quantitative and qualitative profiling	405:442	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	0	35	theme	immune	149:154	arg1	cells					156:160	tumor-infiltrating immune cells	130:160	tumor-infiltrating immune cells	130:160	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	5	36	theme	CD44/CD24	831:839	arg1	patterns					852:859	certain CD44/CD24 expression patterns	823:859	certain CD44/CD24 expression patterns	823:859	This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells.					
29925435	3	37	theme	RESULTS	445:451	arg1	cancer					460:465	RESULTS Breast cancer	445:465	RESULTS Breast cancer	445:465	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	3	37	theme	RESULTS	445:451	arg1	disease					486:492	a heterogeneous disease	470:492	a heterogeneous disease	470:492	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	1	38	theme	living	214:219	arg1	cells					227:231	living tumor cells	214:231	living tumor cells	214:231	OBJECTIVE The use of novel methods to characterize living tumor cells relies on well-conceived biobanks.					
29925435	4	39	theme	classical	656:664	arg1	protocol					678:685	a classical freeze/thaw protocol	654:685	a classical freeze/thaw protocol	654:685	We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells.					
29925435	0	40	theme	tumor-infiltrating	130:147	arg1	cells					156:160	tumor-infiltrating immune cells	130:160	tumor-infiltrating immune cells	130:160	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	5	41	theme	expression	841:850	arg1	patterns					852:859	certain CD44/CD24 expression patterns	823:859	certain CD44/CD24 expression patterns	823:859	This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells.					
29925435	2	42	theme	quantitative	405:416	arg1	profiling					434:442	quantitative and qualitative profiling	405:442	quantitative and qualitative profiling	405:442	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	0	43	theme	breast	56:61	arg1	cells					70:74	triple-negative breast cancer cells	40:74	triple-negative breast cancer cells	40:74	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	6	44	theme	cellular	969:976	arg1	phenotype					978:986	cellular phenotype	969:986	cellular phenotype	969:986	These cryopreservation-driven alterations in cellular phenotype make it impossible to compare fresh and frozen samples from the same patient directly.					
29925435	7	45	theme	expected	1265:1272	arg1	differences					1291:1301	expected inter-individual differences	1265:1301	expected inter-individual differences	1265:1301	Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences.					
29925435	7	46	theme	inter-individual	1274:1289	arg1	differences					1291:1301	expected inter-individual differences	1265:1301	expected inter-individual differences	1265:1301	Moreover, the freeze/thaw process changed the transcriptomic signatures of triple-negative cancer stem cells in such a manner that hierarchical clustering no longer ranked them according to expected inter-individual differences.					
29925435	2	47	from	comparable	382:391	arg1	terms					396:400	terms	396:400	terms of quantitative and qualitative profiling	396:442	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	2	48	theme	freeze/thawed	339:351	arg1	samples					371:377	fresh and freeze/thawed dissociated tumor samples	329:377	fresh and freeze/thawed dissociated tumor samples	329:377	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	0	49	theme	triple-negative	40:54	arg1	cells					70:74	triple-negative breast cancer cells	40:74	triple-negative breast cancer cells	40:74	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	0	50	theme	cells	156:160	arg1	profiles					99:106	tumor CD24/CD44 profiles	83:106	tumor CD24/CD44 profiles	83:106	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	0	50	theme	cells	156:160	arg1	percentage					116:125	the percentage	112:125	the percentage of tumor-infiltrating immune cells	112:160	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	4	51	theme	freeze/thaw	666:676	arg1	protocol					678:685	a classical freeze/thaw protocol	654:685	a classical freeze/thaw protocol	654:685	We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells.					
29925435	8	52	theme	prognostic/predictive	1481:1501	arg1	markers					1503:1509	prognostic/predictive markers	1481:1509	prognostic/predictive markers	1481:1509	Overall, this study suggests that all analyses of living tumor cells should be conducted only using freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers.					
29925435	8	53	theme	scoring	1462:1468	arg1	system					1470:1475	a robust scoring system	1453:1475	a robust scoring system for prognostic/predictive markers	1453:1509	Overall, this study suggests that all analyses of living tumor cells should be conducted only using freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers.					
29925435	2	54	from	terms	396:400	arg1	comparable					382:391	comparable	382:391	comparable	382:391	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	2	54	from	terms	396:400	arg1	composition					314:324	the composition	310:324	the composition of fresh and freeze/thawed dissociated tumor samples	310:377	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	2	55	theme	fresh	329:333	arg1	samples					371:377	fresh and freeze/thawed dissociated tumor samples	329:377	fresh and freeze/thawed dissociated tumor samples	329:377	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	5	56	with	percentage	792:801	arg1	patterns					852:859	certain CD44/CD24 expression patterns	823:859	certain CD44/CD24 expression patterns	823:859	This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells.					
29925435	6	57	theme	same	1052:1055	arg1	patient					1057:1063	the same patient	1048:1063	the same patient	1048:1063	These cryopreservation-driven alterations in cellular phenotype make it impossible to compare fresh and frozen samples from the same patient directly.					
29925435	5	58	theme	cells	917:921	arg1	percentage					877:886	the percentage	873:886	the percentage of tumor-infiltrating immune cells	873:921	This, in turn, changed the percentage of tumor cells with certain CD44/CD24 expression patterns and changed the percentage of tumor-infiltrating immune cells.					
29925435	8	59	theme	robust	1455:1460	arg1	system					1470:1475	a robust scoring system	1453:1475	a robust scoring system for prognostic/predictive markers	1453:1509	Overall, this study suggests that all analyses of living tumor cells should be conducted only using freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers.					
29925435	3	60	theme	basal/triple-negative	525:545	arg1	TNBC					562:565	TNBC	562:565	TNBC	562:565	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	3	60	theme	basal/triple-negative	525:545	arg1	cancer					554:559	basal/triple-negative breast cancer	525:559	basal/triple-negative breast cancer (TNBC)	525:566	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	8	61	theme	dissociated	1412:1422	arg1	tumors					1424:1429	freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers	1404:1509	freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers	1404:1509	Overall, this study suggests that all analyses of living tumor cells should be conducted only using freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers.					
29925435	4	62	theme	living	604:609	arg1	cells					611:615	living cells	604:615	living cells dissociated from TNBC	604:637	We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells.					
29925435	0	63	theme	cells	70:74	arg1	cycle					31:35	cycle	31:35	cycle of triple-negative breast cancer cells	31:74	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	1	64	theme	tumor	221:225	arg1	cells					227:231	living tumor cells	214:231	living tumor cells	214:231	OBJECTIVE The use of novel methods to characterize living tumor cells relies on well-conceived biobanks.					
29925435	3	65	theme	heterogeneous	472:484	arg1	cancer					460:465	RESULTS Breast cancer	445:465	RESULTS Breast cancer	445:465	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	3	65	theme	heterogeneous	472:484	arg1	disease					486:492	a heterogeneous disease	470:492	a heterogeneous disease	470:492	RESULTS Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors.					
29925435	8	66	theme	cells	1367:1371	arg1	analyses					1342:1349	all analyses	1338:1349	all analyses of living tumor cells	1338:1371	Overall, this study suggests that all analyses of living tumor cells should be conducted only using freshly dissociated tumors if we are to generate a robust scoring system for prognostic/predictive markers.					
29925435	4	67	from	reduction	705:713	arg1	number					722:727	the number	718:727	the number of CD45-CD44LowCD24Low tumor cells	718:762	We examined living cells dissociated from TNBC and found that a classical freeze/thaw protocol leads to a marked reduction in the number of CD45-CD44LowCD24Low tumor cells.					
29925435	0	68	theme	cancer	63:68	arg1	cells					70:74	triple-negative breast cancer cells	40:74	triple-negative breast cancer cells	40:74	Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.					
29925435	6	69	theme	cryopreservation-driven	930:952	arg1	alterations					954:964	These cryopreservation-driven alterations	924:964	These cryopreservation-driven alterations in cellular phenotype	924:986	These cryopreservation-driven alterations in cellular phenotype make it impossible to compare fresh and frozen samples from the same patient directly.					
29925435	2	70	theme	samples	371:377	arg1	comparable					382:391	comparable	382:391	comparable	382:391	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	2	70	theme	samples	371:377	arg1	composition					314:324	the composition	310:324	the composition of fresh and freeze/thawed dissociated tumor samples	310:377	Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.					
29925435	1	71	theme	well-conceived	243:256	arg1	biobanks					258:265	well-conceived biobanks	243:265	well-conceived biobanks	243:265	OBJECTIVE The use of novel methods to characterize living tumor cells relies on well-conceived biobanks.					
30316873	7	0	theme	energy	1015:1020	arg1	intake					1022:1027	their energy intake	1009:1027	their energy intake	1009:1027	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	2	1	theme	taste	356:360	arg1	effects					393:399	the pre- or post-absorptive effects	365:399	the pre- or post-absorptive effects of consumption of simple carbohydrate	365:437	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	2	1	theme	taste	356:360	arg1	reward					340:345	the reward	336:345	the reward of sweet taste	336:360	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	9	2	theme	leptin	1173:1178	arg1	injection					1152:1160	An i.p. injection	1144:1160	An i.p. injection of 2 mg/kg leptin	1144:1178	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	7	3	theme	energy	931:936	arg1	intake					938:943	total energy intake	925:943	total energy intake	925:943	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	9	4	theme	food	1190:1193	arg1	intake					1195:1200	food intake	1190:1200	food intake of NS, HS and Sacc rats	1190:1224	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	3	5	theme	%	613:613	arg1	solution					624:631	20% SolCarb® solution	611:631	20% SolCarb® solution	611:631	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	3	6	theme	67	522:523	arg1	%					524:524	%	524:524	%	524:524	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	0	7	theme	calorie-free	129:140	arg1	solution					148:155	not calorie-free sweet solution	125:155	not calorie-free sweet solution	125:155	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	10	8	theme	serum	1445:1449	arg1	concentrations					1458:1471	serum leptin concentrations	1445:1471	serum leptin concentrations	1445:1471	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	6	9	theme	calories	812:819	arg1	number					802:807	the same number	793:807	the same number of calories from Day 15 to the end of the experiment	793:860	SolCarb® and LS rats drank the same number of calories from Day 15 to the end of the experiment.					
30316873	7	10	theme	dry	893:895	arg1	food					897:900	dry food	893:900	dry food	893:900	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	10	11	contain	had	1411:1413	arg2	concentrations					1458:1471	serum leptin concentrations	1445:1471	serum leptin concentrations	1445:1471	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	10	11	contain	had	1411:1413	arg1	all					1371:1373	all	1371:1373	all	1371:1373	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	10	11	contain	had	1411:1413	arg1	rats					1382:1385	the rats	1378:1385	the rats	1378:1385	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	10	11	contain	had	1411:1413	arg2	composition					1429:1439	the same body composition	1415:1439	the same body composition	1415:1439	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	7	12	theme	LS	876:877	arg1	rats					879:882	SolCarb® and LS rats	863:882	SolCarb® and LS rats	863:882	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	3	13	theme	%	524:524	arg1	calories					526:533	67% calories	522:533	67% calories	522:533	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	5	14	theme	LS	717:718	arg1	rats					720:723	LS rats	717:723	LS rats	717:723	Sacc rats initially drank more than LS rats, but intakes were the same after Day 20.					
30316873	7	15	dep	NS	970:971	arg1	groups					986:991	groups	986:991	groups	986:991	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	1	16	theme	%	173:173	arg1	solution					183:190	30% sucrose solution	171:190	30% sucrose solution	171:190	Rats offered 30% sucrose solution plus chow or a sucrose-free diet develop leptin resistance within 4 weeks.					
30316873	0	17	from	Development	0:10	arg1	rats					53:56	sucrose drinking rats	36:56	sucrose drinking rats	36:56	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	0	18	theme	carbohydrate-containing	87:109	arg1	solutions					111:119	consuming carbohydrate-containing solutions	77:119	consuming carbohydrate-containing solutions	77:119	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	9	19	theme	LS	1265:1266	arg1	rats					1280:1283	LS or SolCarb® rats	1265:1283	rats	1280:1283	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	7	20	dep	greater	949:955	arg1	carbohydrate					1038:1049	carbohydrate	1038:1049	carbohydrate	1038:1049	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	9	21	theme	SolCarb®	1271:1278	arg1	rats					1280:1283	LS or SolCarb® rats	1265:1283	rats	1280:1283	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	8	22	theme	own	1131:1133	arg1	control					1135:1141	its own control	1127:1141	its own control	1127:1141	Leptin responsiveness was tested on Days 27 and 32 with each rat acting as its own control.					
30316873	2	23	theme	post-absorptive	377:391	arg1	effects					393:399	the pre- or post-absorptive effects	365:399	the pre- or post-absorptive effects of consumption of simple carbohydrate	365:437	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	2	24	theme	carbohydrate	426:437	arg1	consumption					404:414	consumption	404:414	consumption of simple carbohydrate	404:437	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	0	25	theme	leptin	15:20	arg1	resistance					22:31	leptin resistance	15:31	leptin resistance in sucrose drinking rats	15:56	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	11	26	theme	resistance	1610:1619	arg1	development					1588:1598	the development	1584:1598	the development of leptin resistance	1584:1619	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	9	27	dep	NS	1205:1206	arg1	rats					1221:1224	rats	1221:1224	rats	1221:1224	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	10	28	theme	experiment	1360:1369	arg1	end					1349:1351	the end	1345:1351	the end of the experiment	1345:1369	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	5	29	theme	Sacc	681:684	arg1	rats					686:689	Sacc rats	681:689	Sacc rats	681:689	Sacc rats initially drank more than LS rats, but intakes were the same after Day 20.					
30316873	9	30	theme	Sacc	1306:1309	arg1	solution					1332:1339	Sacc, sucrose or SolCarb® solution	1306:1339	solution	1332:1339	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	3	31	contain	containing	511:520	arg1	HS					547:548	HS	547:548	HS	547:548	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	3	31	contain	containing	511:520	arg2	calories					526:533	67% calories	522:533	67% calories	522:533	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	3	31	contain	containing	511:520	arg1	diet					506:509	a diet	504:509	a diet containing 67% calories as sucrose (HS)	504:549	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	2	32	theme	sweet	350:354	arg1	taste					356:360	sweet taste	350:360	sweet taste	350:360	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	0	33	theme	drinking	44:51	arg1	rats					53:56	sucrose drinking rats	36:56	sucrose drinking rats	36:56	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	11	34	contain	containing	1524:1533	arg2	solution					1548:1555	carbohydrate solution	1535:1555	carbohydrate solution	1535:1555	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	11	34	contain	containing	1524:1533	arg2	taste					1571:1575	sweet taste	1565:1575	sweet taste	1565:1575	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	11	34	contain	containing	1524:1533	arg1	calorie					1516:1522	a calorie	1514:1522	a calorie containing carbohydrate solution and not sweet taste	1514:1575	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	9	35	theme	sucrose	1312:1318	arg1	solution					1332:1339	Sacc, sucrose or SolCarb® solution	1306:1339	solution	1332:1339	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	3	36	theme	30	567:568	arg1	%					569:569	%	569:569	%	569:569	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	3	37	theme	sucrose-free	480:491	arg1	NS					499:500	NS	499:500	NS	499:500	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	3	37	theme	sucrose-free	480:491	arg1	diet					493:496	a sucrose-free diet	478:496	a sucrose-free diet (NS)	478:501	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	10	38	theme	same	1419:1422	arg1	composition					1429:1439	the same body composition	1415:1439	the same body composition	1415:1439	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	9	39	theme	SolCarb®	1323:1330	arg1	solution					1332:1339	Sacc, sucrose or SolCarb® solution	1306:1339	solution	1332:1339	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	7	40	dep	less	888:891	arg1	food					897:900	dry food	893:900	dry food	893:900	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	3	41	theme	%	589:589	arg1	Sacc					602:605	Sacc	602:605	Sacc	602:605	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	3	41	theme	%	589:589	arg1	saccharin					591:599	0.03% saccharin	585:599	0.03% saccharin (Sacc)	585:606	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	2	42	theme	pre-	369:372	arg1	effects					393:399	the pre- or post-absorptive effects	365:399	the pre- or post-absorptive effects of consumption of simple carbohydrate	365:437	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	3	43	theme	20	611:612	arg1	%					613:613	%	613:613	%	613:613	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	9	44	theme	2 mg/kg	1165:1171	arg1	leptin					1173:1178	2 mg/kg leptin	1165:1178	2 mg/kg leptin	1165:1178	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	6	45	theme	LS	779:780	arg1	rats					782:785	SolCarb® and LS rats	766:785	SolCarb® and LS rats	766:785	SolCarb® and LS rats drank the same number of calories from Day 15 to the end of the experiment.					
30316873	7	46	theme	intake	1022:1027	arg1	%					1004:1004	80%	1002:1004	80% of their energy intake	1002:1027	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	7	46	theme	intake	1022:1027	arg1	intake					1022:1027	their energy intake	1009:1027	their energy intake	1009:1027	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	7	47	theme	total	925:929	arg1	intake					938:943	total energy intake	925:943	total energy intake	925:943	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	2	48	theme	leptin	298:303	arg1	resistance					305:314	leptin resistance	298:314	leptin resistance	298:314	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	6	49	theme	SolCarb®	766:773	arg1	rats					782:785	SolCarb® and LS rats	766:785	SolCarb® and LS rats	766:785	SolCarb® and LS rats drank the same number of calories from Day 15 to the end of the experiment.					
30316873	9	50	theme	energy	1248:1253	arg1	intake					1255:1260	energy intake	1248:1260	energy intake of LS or SolCarb® rats	1248:1283	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	10	51	theme	body	1424:1427	arg1	composition					1429:1439	the same body composition	1415:1439	the same body composition	1415:1439	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	1	52	theme	sucrose	175:181	arg1	solution					183:190	30% sucrose solution	171:190	30% sucrose solution	171:190	Rats offered 30% sucrose solution plus chow or a sucrose-free diet develop leptin resistance within 4 weeks.					
30316873	3	53	theme	SolCarb®	615:622	arg1	solution					624:631	20% SolCarb® solution	611:631	20% SolCarb® solution	611:631	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	7	54	theme	other	907:911	arg1	groups					913:918	other groups	907:918	other groups	907:918	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	9	55	theme	NS	1205:1206	arg1	intake					1195:1200	food intake	1190:1200	food intake of NS, HS and Sacc rats	1190:1224	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	0	56	theme	sweet	142:146	arg1	solution					148:155	not calorie-free sweet solution	125:155	not calorie-free sweet solution	125:155	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	10	57	theme	leptin	1451:1456	arg1	concentrations					1458:1471	serum leptin concentrations	1445:1471	serum leptin concentrations	1445:1471	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	9	58	theme	HS	1209:1210	arg1	intake					1195:1200	food intake	1190:1200	food intake of NS, HS and Sacc rats	1190:1224	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	3	59	theme	NS	554:555	arg1	diet					557:560	NS diet	554:560	NS diet	554:560	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	3	59	theme	NS	554:555	arg1	LS					580:581	LS	580:581	LS	580:581	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	9	60	theme	Sacc	1216:1219	arg1	intake					1195:1200	food intake	1190:1200	food intake of NS, HS and Sacc rats	1190:1224	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	8	61	theme	Leptin	1052:1057	arg1	responsiveness					1059:1072	Leptin responsiveness	1052:1072	Leptin responsiveness	1052:1072	Leptin responsiveness was tested on Days 27 and 32 with each rat acting as its own control.					
30316873	11	62	theme	calorie	1516:1522	arg1	consumption					1499:1509	consumption	1499:1509	consumption of a calorie containing carbohydrate solution and not sweet taste	1499:1575	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	7	63	theme	SolCarb®	863:870	arg1	rats					879:882	SolCarb® and LS rats	863:882	SolCarb® and LS rats	863:882	SolCarb® and LS rats ate less dry food than other groups, but total energy intake was greater than that of NS, HS and Sacc groups and over 80% of their energy intake was from carbohydrate.					
30316873	6	64	theme	same	797:800	arg1	number					802:807	the same number	793:807	the same number of calories from Day 15 to the end of the experiment	793:860	SolCarb® and LS rats drank the same number of calories from Day 15 to the end of the experiment.					
30316873	3	65	theme	%	569:569	arg1	sucrose					571:577	30% sucrose	567:577	30% sucrose	567:577	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	11	66	theme	reward	1711:1716	arg1	inhibition					1697:1706	inhibition	1697:1706	inhibition of reward by leptin	1697:1726	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	11	67	theme	hunger	1681:1686	arg1	inhibition					1667:1676	inhibition	1667:1676	inhibition of hunger than for inhibition of reward by leptin	1667:1726	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	6	68	theme	experiment	851:860	arg1	end					840:842	the end	836:842	the end of the experiment	836:860	SolCarb® and LS rats drank the same number of calories from Day 15 to the end of the experiment.					
30316873	0	69	from	resistance	22:31	arg1	rats					53:56	sucrose drinking rats	36:56	sucrose drinking rats	36:56	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	0	70	theme	consuming	77:85	arg1	solutions					111:119	consuming carbohydrate-containing solutions	77:119	consuming carbohydrate-containing solutions	77:119	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	11	71	theme	sweet	1565:1569	arg1	taste					1571:1575	sweet taste	1565:1575	sweet taste	1565:1575	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	1	72	theme	30	171:172	arg1	%					173:173	%	173:173	%	173:173	Rats offered 30% sucrose solution plus chow or a sucrose-free diet develop leptin resistance within 4 weeks.					
30316873	0	73	from	rats	53:56	arg1	Development					0:10	Development	0:10	Development of leptin resistance in sucrose drinking rats	0:56	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	10	74	dep	sensitive	1400:1408	arg1	had					1411:1413	had	1411:1413	had the same body composition and serum leptin concentrations	1411:1471	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	4	75	theme	maltodextrin	660:671	arg1	SolCarb®					646:653	SolCarb®	646:653	SolCarb®	646:653	SolCarb® is a maltodextrin powder.					
30316873	4	75	theme	maltodextrin	660:671	arg1	powder					673:678	a maltodextrin powder	658:678	a maltodextrin powder	658:678	SolCarb® is a maltodextrin powder.					
30316873	3	76	theme	Dawley	453:458	arg1	rats					460:463	Male Sprague Dawley rats	440:463	Male Sprague Dawley rats	440:463	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	9	77	theme	rats	1280:1283	arg1	intake					1255:1260	energy intake	1248:1260	energy intake of LS or SolCarb® rats	1248:1283	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	3	78	theme	Male	440:443	arg1	rats					460:463	Male Sprague Dawley rats	440:463	Male Sprague Dawley rats	440:463	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	0	79	theme	resistance	22:31	arg1	Development					0:10	Development	0:10	Development of leptin resistance in sucrose drinking rats	0:56	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	11	80	theme	lower	1647:1651	arg1	threshold					1653:1661	lower threshold	1647:1661	lower threshold for inhibition of hunger than for inhibition of reward by leptin	1647:1726	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	9	81	contain	had	1231:1233	arg1	injection					1152:1160	An i.p. injection	1144:1160	An i.p. injection of 2 mg/kg leptin	1144:1178	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	9	81	contain	had	1231:1233	arg2	effect					1238:1243	no effect	1235:1243	no effect	1235:1243	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	8	82	with	Days	1088:1091	arg1	rat					1113:1115	each rat	1108:1115	each rat acting as its own control	1108:1141	Leptin responsiveness was tested on Days 27 and 32 with each rat acting as its own control.					
30316873	2	83	theme	simple	419:424	arg1	carbohydrate					426:437	simple carbohydrate	419:437	simple carbohydrate	419:437	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	0	84	theme	sucrose	36:42	arg1	rats					53:56	sucrose drinking rats	36:56	sucrose drinking rats	36:56	Development of leptin resistance in sucrose drinking rats is associated with consuming carbohydrate-containing solutions and not calorie-free sweet solution.					
30316873	1	85	theme	sucrose-free	207:218	arg1	diet					220:223	a sucrose-free diet	205:223	a sucrose-free diet	205:223	Rats offered 30% sucrose solution plus chow or a sucrose-free diet develop leptin resistance within 4 weeks.					
30316873	6	86	from	Day	826:828	arg1	number					802:807	the same number	793:807	the same number of calories from Day 15 to the end of the experiment	793:860	SolCarb® and LS rats drank the same number of calories from Day 15 to the end of the experiment.					
30316873	6	86	from	Day	826:828	arg1	calories					812:819	calories	812:819	calories from Day 15	812:831	SolCarb® and LS rats drank the same number of calories from Day 15 to the end of the experiment.					
30316873	2	87	theme	consumption	404:414	arg1	effects					393:399	the pre- or post-absorptive effects	365:399	the pre- or post-absorptive effects of consumption of simple carbohydrate	365:437	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	2	87	theme	consumption	404:414	arg1	reward					340:345	the reward	336:345	the reward of sweet taste	336:360	This experiment tested whether leptin resistance was associated with the reward of sweet taste or the pre- or post-absorptive effects of consumption of simple carbohydrate.					
30316873	10	88	from	end	1349:1351	arg1	sensitive					1400:1408	sensitive	1400:1408	sensitive	1400:1408	At the end of the experiment all of the rats were insulin sensitive, had the same body composition and serum leptin concentrations.					
30316873	6	89	from	number	802:807	arg1	Day					826:828	Day 15	826:831	Day 15	826:831	SolCarb® and LS rats drank the same number of calories from Day 15 to the end of the experiment.					
30316873	3	90	theme	0.03	585:588	arg1	%					589:589	%	589:589	%	589:589	Male Sprague Dawley rats were offered a sucrose-free diet (NS), a diet containing 67% calories as sucrose (HS) or NS diet plus 30% sucrose (LS), 0.03% saccharin (Sacc) or 20% SolCarb® solution for 38 days.					
30316873	11	91	theme	carbohydrate	1535:1546	arg1	solution					1548:1555	carbohydrate solution	1535:1555	carbohydrate solution	1535:1555	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30316873	1	92	theme	leptin	233:238	arg1	resistance					240:249	leptin resistance	233:249	leptin resistance	233:249	Rats offered 30% sucrose solution plus chow or a sucrose-free diet develop leptin resistance within 4 weeks.					
30316873	9	93	theme	solution	1332:1339	arg1	consumption					1291:1301	consumption	1291:1301	consumption of Sacc, sucrose or SolCarb® solution	1291:1339	An i.p. injection of 2 mg/kg leptin inhibited food intake of NS, HS and Sacc rats, but had no effect on energy intake of LS or SolCarb® rats or on consumption of Sacc, sucrose or SolCarb® solution.					
30316873	11	94	theme	leptin	1603:1608	arg1	resistance					1610:1619	leptin resistance	1603:1619	leptin resistance	1603:1619	These data indicate that consumption of a calorie containing carbohydrate solution and not sweet taste drives the development of leptin resistance and suggest that there is lower threshold for inhibition of hunger than for inhibition of reward by leptin.					
30007735	2	0	theme	basal	542:546	arg1	additive					524:531	additive	524:531	additive	524:531	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	0	theme	basal	542:546	arg1	paniculata					573:582	a basal diet + 1.5% Andrographis paniculata	540:582	a basal diet + 1.5% Andrographis paniculata leaves	540:589	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	10	1	dep	juiciness	1451:1459	arg1	meat					1553:1556	meat	1553:1556	meat	1553:1556	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	10	1	dep	juiciness	1451:1459	arg1	7.89					1520:1523	7.89	1520:1523	7.89	1520:1523	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	10	2	theme	APL	1503:1505	arg1	APW					1530:1532	APW	1530:1532	APW (7.90, 7.08, 7.77)	1530:1551	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	10	2	theme	APL	1503:1505	arg1	tenderness					1462:1471	tenderness	1462:1471	tenderness	1462:1471	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	10	2	theme	APL	1503:1505	arg1	juiciness					1451:1459	juiciness	1451:1459	juiciness	1451:1459	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	10	2	theme	APL	1503:1505	arg1	acceptability					1486:1498	overall acceptability	1478:1498	overall acceptability of APL	1478:1505	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	1	3	theme	paniculata	253:262	arg1	parts					231:235	different parts	221:235	different parts of Andrographis paniculata	221:262	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	10	4	theme	AP0	1597:1599	arg1	meat					1620:1623	the AP0 (5.38, 5.95, 5.41) meat	1593:1623	the AP0 (5.38, 5.95, 5.41) meat	1593:1623	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	1	5	from	microbiota	297:306	arg1	goats					383:387	goats	383:387	goats	383:387	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	6	theme	fatty	267:271	arg1	microbiota					297:306	microbiota	297:306	microbiota	297:306	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	6	theme	fatty	267:271	arg1	acids					273:277	fatty acids	267:277	fatty acids	267:277	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	6	theme	fatty	267:271	arg1	oxidation					286:294	lipid oxidation	280:294	lipid oxidation	280:294	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	6	theme	fatty	267:271	arg1	attributes					320:329	quality attributes	312:329	quality attributes	312:329	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	11	7	theme	Total	1626:1630	arg1	counts					1639:1644	Total viable counts	1626:1644	Total viable counts	1626:1644	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	12	8	theme	antimicrobial	1816:1828	arg1	potential					1830:1838	higher (p < .05) antimicrobial potential	1799:1838	higher (p < .05) antimicrobial potential	1799:1838	The APL exhibited higher (p < .05) antimicrobial potential than the APW.					
30007735	12	8	theme	antimicrobial	1816:1828	arg1	p < .05					1807:1813	p < .05	1807:1813	p < .05	1807:1813	The APL exhibited higher (p < .05) antimicrobial potential than the APW.					
30007735	2	9	theme	%	558:558	arg1	additive					524:531	additive	524:531	additive	524:531	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	9	theme	%	558:558	arg1	paniculata					573:582	a basal diet + 1.5% Andrographis paniculata	540:582	a basal diet + 1.5% Andrographis paniculata leaves	540:589	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	11	10	theme	AP0	1746:1748	arg1	meat					1750:1753	AP0 meat	1746:1753	AP0 meat	1746:1753	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	6	11	dep	APL	984:986	arg1	meat					996:999	meat	996:999	meat	996:999	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	14	12	from	counts	2090:2095	arg1	chevon					2100:2105	chevon	2100:2105	chevon	2100:2105	Dietary APL and APW enhanced the beneficial fatty acids, quality attributes and oxidative stability, and reduced microbial counts in chevon.					
30007735	2	13	dep	paniculata	573:582	arg1	leaves					584:589	leaves	584:589	leaves	584:589	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	5	14	theme	higher	789:794	arg1	concentration					806:818	higher (p < .05) concentration	789:818	higher (p < .05) concentration of C16:0 and C18:0	789:837	The AP0 meat had higher (p < .05) concentration of C16:0 and C18:0 than the APW and APL meat.					
30007735	10	15	dep	higher	1563:1568	arg1	p < .05					1571:1577	p < .05	1571:1577	p < .05	1571:1577	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	5	16	dep	APW	848:850	arg1	the					844:846	the	844:846	the	844:846	The AP0 meat had higher (p < .05) concentration of C16:0 and C18:0 than the APW and APL meat.					
30007735	1	17	theme	supplementation	202:216	arg1	effects					183:189	The effects	179:189	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats	179:387	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	2	18	theme	paniculata	633:642	arg1	APW					657:659	APW	657:659	APW	657:659	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	18	theme	paniculata	633:642	arg1	plant					650:654	a basal diet + 1.5% Andrographis paniculata whole plant	600:654	a basal diet + 1.5% Andrographis paniculata whole plant (APW)	600:660	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	13	19	theme	lipid	1919:1923	arg1	oxidation					1925:1933	lipid oxidation	1919:1933	lipid oxidation	1919:1933	Chill storage affected (p < .05) the physicochemical properties, lipid oxidation and microbial counts in chevon.					
30007735	1	20	from	effects	183:189	arg1	microbiota					297:306	microbiota	297:306	microbiota	297:306	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	20	from	effects	183:189	arg1	acids					273:277	fatty acids	267:277	fatty acids	267:277	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	20	from	effects	183:189	arg1	oxidation					286:294	lipid oxidation	280:294	lipid oxidation	280:294	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	20	from	effects	183:189	arg1	attributes					320:329	quality attributes	312:329	quality attributes	312:329	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	8	21	dep	lower	1187:1191	arg1	p < .05					1194:1200	p < .05	1194:1200	p < .05	1194:1200	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	0	22	from	oxidation	101:109	arg1	goats					172:176	goats	172:176	goats	172:176	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	2	23	theme	%	618:618	arg1	APW					657:659	APW	657:659	APW	657:659	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	23	theme	%	618:618	arg1	plant					650:654	a basal diet + 1.5% Andrographis paniculata whole plant	600:654	a basal diet + 1.5% Andrographis paniculata whole plant (APW)	600:660	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	0	24	theme	longissimus	150:160	arg1	muscle					162:167	longissimus muscle	150:167	longissimus muscle	150:167	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	10	25	theme	Sensory	1432:1438	arg1	scores					1440:1445	Sensory scores	1432:1445	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat	1432:1556	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	14	26	theme	oxidative	2047:2055	arg1	stability					2057:2065	oxidative stability	2047:2065	oxidative stability	2047:2065	Dietary APL and APW enhanced the beneficial fatty acids, quality attributes and oxidative stability, and reduced microbial counts in chevon.					
30007735	11	27	theme	Enterobacteriacea	1703:1719	arg1	populations					1650:1660	populations	1650:1660	populations	1650:1660	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	11	27	theme	Enterobacteriacea	1703:1719	arg1	counts					1639:1644	Total viable counts	1626:1644	Total viable counts	1626:1644	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	7	28	theme	acid	1117:1120	arg1	bacteria					1122:1129	lactic acid bacteria	1110:1129	lactic acid bacteria	1110:1129	Diets had no effect (p > .05) on muscle glycogen, pH, drip loss, chemical composition and lactic acid bacteria count.					
30007735	2	29	theme	20.18 ± 0.19 kg	450:464	arg1	BW					466:467	20.18 ± 0.19 kg BW	450:467	20.18 ± 0.19 kg BW	450:467	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	0	30	theme	fatty	82:86	arg1	acids					88:92	the fatty acids	78:92	the fatty acids	78:92	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	1	31	theme	Longissimus	334:344	arg1	muscle					373:378	Longissimus thoracis et lumborum (LTL) muscle	334:378	Longissimus thoracis et lumborum (LTL) muscle	334:378	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	8	32	theme	0.12 mg	1228:1234	arg1	MDA/kg					1236:1241	0.12 mg MDA/kg	1228:1241	0.12 mg MDA/kg	1228:1241	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	32	theme	0.12 mg	1228:1234	arg1	%					1216:1216	23.98%	1211:1216	23.98%	1211:1216	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	33	dep	AP0	1305:1307	arg1	1.13 kg					1318:1324	1.13 kg	1318:1324	1.13 kg	1318:1324	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	33	dep	AP0	1305:1307	arg1	MDA/kg					1335:1340	0.23 mg MDA/kg	1327:1340	0.23 mg MDA/kg	1327:1340	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	33	dep	AP0	1305:1307	arg1	%					1315:1315	26.49%	1310:1315	26.49%	1310:1315	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	2	34	dep	4 months	436:443	arg1	BW					466:467	20.18 ± 0.19 kg BW	450:467	20.18 ± 0.19 kg BW	450:467	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	5	35	theme	C18:0	833:837	arg1	concentration					806:818	higher (p < .05) concentration	789:818	higher (p < .05) concentration of C16:0 and C18:0	789:837	The AP0 meat had higher (p < .05) concentration of C16:0 and C18:0 than the APW and APL meat.					
30007735	14	36	theme	fatty	2011:2015	arg1	acids					2017:2021	the beneficial fatty acids	1996:2021	the beneficial fatty acids	1996:2021	Dietary APL and APW enhanced the beneficial fatty acids, quality attributes and oxidative stability, and reduced microbial counts in chevon.					
30007735	6	37	theme	C18:1n-9	917:924	arg1	higher					964:969	higher	964:969	higher	964:969	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	6	37	theme	C18:1n-9	917:924	arg1	concentrations					870:883	The concentrations	866:883	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3	866:957	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	2	38	theme	Boer	424:427	arg1	four					411:414	four	411:414	four	411:414	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	38	theme	Boer	424:427	arg1	4 months					436:443	4 months	436:443	4 months old	436:447	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	38	theme	Boer	424:427	arg1	bucks					429:433	entire Boer bucks	417:433	entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW)	417:468	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	10	39	dep	APW	1530:1532	arg1	7.77					1547:1550	7.77	1547:1550	7.77	1547:1550	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	2	40	theme	basal	602:606	arg1	APW					657:659	APW	657:659	APW	657:659	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	40	theme	basal	602:606	arg1	plant					650:654	a basal diet + 1.5% Andrographis paniculata whole plant	600:654	a basal diet + 1.5% Andrographis paniculata whole plant (APW)	600:660	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	0	41	theme	Dietary	0:6	arg1	supplementation					8:22	Dietary supplementation	0:22	Dietary supplementation of different parts of Andrographis paniculata	0:68	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	6	42	theme	CLA	912:914	arg1	higher					964:969	higher	964:969	higher	964:969	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	6	42	theme	CLA	912:914	arg1	concentrations					870:883	The concentrations	866:883	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3	866:957	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	6	43	theme	AP0	1010:1012	arg1	meat					1014:1017	the AP0 meat	1006:1017	the AP0 meat	1006:1017	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	8	44	theme	shear	1152:1156	arg1	force					1158:1162	shear force	1152:1162	shear force	1152:1162	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	1	45	theme	lipid	280:284	arg1	acids					273:277	fatty acids	267:277	fatty acids	267:277	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	45	theme	lipid	280:284	arg1	oxidation					286:294	lipid oxidation	280:294	lipid oxidation	280:294	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	14	46	theme	beneficial	2000:2009	arg1	acids					2017:2021	the beneficial fatty acids	1996:2021	the beneficial fatty acids	1996:2021	Dietary APL and APW enhanced the beneficial fatty acids, quality attributes and oxidative stability, and reduced microbial counts in chevon.					
30007735	5	47	theme	C16:0	823:827	arg1	concentration					806:818	higher (p < .05) concentration	789:818	higher (p < .05) concentration of C16:0 and C18:0	789:837	The AP0 meat had higher (p < .05) concentration of C16:0 and C18:0 than the APW and APL meat.					
30007735	2	48	theme	old	445:447	arg1	4 months					436:443	4 months	436:443	4 months old	436:447	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	48	theme	old	445:447	arg1	bucks					429:433	entire Boer bucks	417:433	entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW)	417:468	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	0	49	theme	parts	37:41	arg1	supplementation					8:22	Dietary supplementation	0:22	Dietary supplementation of different parts of Andrographis paniculata	0:68	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	8	50	dep	APL	1206:1208	arg1	meat					1286:1289	meat	1286:1289	meat	1286:1289	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	50	dep	APL	1206:1208	arg1	MDA/kg					1236:1241	0.12 mg MDA/kg	1228:1241	0.12 mg MDA/kg	1228:1241	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	50	dep	APL	1206:1208	arg1	%					1216:1216	23.98%	1211:1216	23.98%	1211:1216	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	50	dep	APL	1206:1208	arg1	0.76 kg					1219:1225	0.76 kg	1219:1225	0.76 kg	1219:1225	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	7	51	theme	drip	1074:1077	arg1	loss					1079:1082	drip loss	1074:1082	drip loss	1074:1082	Diets had no effect (p > .05) on muscle glycogen, pH, drip loss, chemical composition and lactic acid bacteria count.					
30007735	11	52	dep	higher	1726:1731	arg1	p < .05					1734:1740	p < .05	1734:1740	p < .05	1734:1740	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	0	53	theme	Andrographis	46:57	arg1	paniculata					59:68	Andrographis paniculata	46:68	Andrographis paniculata	46:68	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	11	54	theme	viable	1632:1637	arg1	counts					1639:1644	Total viable counts	1626:1644	Total viable counts	1626:1644	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	10	55	theme	overall	1478:1484	arg1	acceptability					1486:1498	overall acceptability	1478:1498	overall acceptability of APL	1478:1505	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	14	56	theme	Dietary	1967:1973	arg1	APL					1975:1977	Dietary APL	1967:1977	Dietary APL	1967:1977	Dietary APL and APW enhanced the beneficial fatty acids, quality attributes and oxidative stability, and reduced microbial counts in chevon.					
30007735	6	57	theme	C20:5n-3	950:957	arg1	higher					964:969	higher	964:969	higher	964:969	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	6	57	theme	C20:5n-3	950:957	arg1	concentrations					870:883	The concentrations	866:883	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3	866:957	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	6	58	theme	total	888:892	arg1	C18:1trans					894:903	total C18:1trans	888:903	total C18:1trans	888:903	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	11	59	dep	APL	1763:1765	arg1	meat					1775:1778	meat	1775:1778	meat	1775:1778	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	8	60	theme	0.23 mg	1327:1333	arg1	MDA/kg					1335:1340	0.23 mg MDA/kg	1327:1340	0.23 mg MDA/kg	1327:1340	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	60	theme	0.23 mg	1327:1333	arg1	%					1315:1315	26.49%	1310:1315	26.49%	1310:1315	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	1	61	theme	parts	231:235	arg1	supplementation					202:216	dietary supplementation	194:216	dietary supplementation of different parts of Andrographis paniculata	194:262	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	62	from	attributes	320:329	arg1	goats					383:387	goats	383:387	goats	383:387	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	6	63	theme	C18:3n-3	937:944	arg1	higher					964:969	higher	964:969	higher	964:969	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	6	63	theme	C18:3n-3	937:944	arg1	concentrations					870:883	The concentrations	866:883	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3	866:957	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	0	64	from	microbiota	112:121	arg1	goats					172:176	goats	172:176	goats	172:176	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	11	65	theme	coli	1694:1697	arg1	populations					1650:1660	populations	1650:1660	populations	1650:1660	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	11	65	theme	coli	1694:1697	arg1	counts					1639:1644	Total viable counts	1626:1644	Total viable counts	1626:1644	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	1	66	theme	Andrographis	240:251	arg1	paniculata					253:262	Andrographis paniculata	240:262	Andrographis paniculata	240:262	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	6	67	theme	C18:2n-6	927:934	arg1	higher					964:969	higher	964:969	higher	964:969	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	6	67	theme	C18:2n-6	927:934	arg1	concentrations					870:883	The concentrations	866:883	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3	866:957	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	4	68	theme	chill	757:761	arg1	storage					763:769	a 7 d chill storage	751:769	a 7 d chill storage	751:769	The LTL muscle was subjected to a 7 d chill storage.					
30007735	0	69	from	attributes	136:145	arg1	goats					172:176	goats	172:176	goats	172:176	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	2	70	theme	Andrographis	560:571	arg1	additive					524:531	additive	524:531	additive	524:531	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	70	theme	Andrographis	560:571	arg1	paniculata					573:582	a basal diet + 1.5% Andrographis paniculata	540:582	a basal diet + 1.5% Andrographis paniculata leaves	540:589	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	71	theme	diet + 1.5	548:557	arg1	%					558:558	%	558:558	%	558:558	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	13	72	theme	microbial	1939:1947	arg1	counts					1949:1954	microbial counts	1939:1954	microbial counts	1939:1954	Chill storage affected (p < .05) the physicochemical properties, lipid oxidation and microbial counts in chevon.					
30007735	13	73	theme	Chill	1854:1858	arg1	storage					1860:1866	Chill storage	1854:1866	Chill storage	1854:1866	Chill storage affected (p < .05) the physicochemical properties, lipid oxidation and microbial counts in chevon.					
30007735	2	74	theme	basal	505:509	arg1	APL					592:594	APL	592:594	APL	592:594	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	74	theme	basal	505:509	arg1	diet					511:514	a basal diet	503:514	a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL)	503:595	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	0	75	from	acids	88:92	arg1	goats					172:176	goats	172:176	goats	172:176	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	0	76	theme	quality	128:134	arg1	attributes					136:145	quality attributes	128:145	quality attributes	128:145	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	9	77	theme	Meat	1349:1352	arg1	redness					1354:1360	Meat redness	1349:1360	Meat redness	1349:1360	Meat redness was higher (p < .05) in APL (13.49) and APW (12.98) than AP0 (10.86).					
30007735	1	78	theme	dietary	194:200	arg1	supplementation					202:216	dietary supplementation	194:216	dietary supplementation of different parts of Andrographis paniculata	194:262	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	14	79	theme	microbial	2080:2088	arg1	counts					2090:2095	microbial counts	2080:2095	microbial counts in chevon	2080:2105	Dietary APL and APW enhanced the beneficial fatty acids, quality attributes and oxidative stability, and reduced microbial counts in chevon.					
30007735	2	80	theme	whole	644:648	arg1	APW					657:659	APW	657:659	APW	657:659	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	80	theme	whole	644:648	arg1	plant					650:654	a basal diet + 1.5% Andrographis paniculata whole plant	600:654	a basal diet + 1.5% Andrographis paniculata whole plant (APW)	600:660	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	81	theme	Andrographis	620:631	arg1	APW					657:659	APW	657:659	APW	657:659	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	81	theme	Andrographis	620:631	arg1	plant					650:654	a basal diet + 1.5% Andrographis paniculata whole plant	600:654	a basal diet + 1.5% Andrographis paniculata whole plant (APW)	600:660	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	0	82	theme	muscle	162:167	arg1	microbiota					112:121	microbiota	112:121	microbiota	112:121	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	0	82	theme	muscle	162:167	arg1	acids					88:92	the fatty acids	78:92	the fatty acids	78:92	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	0	82	theme	muscle	162:167	arg1	oxidation					101:109	lipid oxidation	95:109	lipid oxidation	95:109	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	0	82	theme	muscle	162:167	arg1	attributes					136:145	quality attributes	128:145	quality attributes	128:145	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	5	83	contain	had	785:787	arg1	meat					780:783	The AP0 meat	772:783	The AP0 meat	772:783	The AP0 meat had higher (p < .05) concentration of C16:0 and C18:0 than the APW and APL meat.					
30007735	5	83	contain	had	785:787	arg2	concentration					806:818	higher (p < .05) concentration	789:818	higher (p < .05) concentration of C16:0 and C18:0	789:837	The AP0 meat had higher (p < .05) concentration of C16:0 and C18:0 than the APW and APL meat.					
30007735	9	84	dep	higher	1366:1371	arg1	p < .05					1374:1380	p < .05	1374:1380	p < .05	1374:1380	Meat redness was higher (p < .05) in APL (13.49) and APW (12.98) than AP0 (10.86).					
30007735	5	85	theme	AP0	776:778	arg1	meat					780:783	The AP0 meat	772:783	The AP0 meat	772:783	The AP0 meat had higher (p < .05) concentration of C16:0 and C18:0 than the APW and APL meat.					
30007735	14	86	theme	quality	2024:2030	arg1	attributes					2032:2041	quality attributes	2024:2041	quality attributes	2024:2041	Dietary APL and APW enhanced the beneficial fatty acids, quality attributes and oxidative stability, and reduced microbial counts in chevon.					
30007735	12	87	theme	higher	1799:1804	arg1	potential					1830:1838	higher (p < .05) antimicrobial potential	1799:1838	higher (p < .05) antimicrobial potential	1799:1838	The APL exhibited higher (p < .05) antimicrobial potential than the APW.					
30007735	12	87	theme	higher	1799:1804	arg1	p < .05					1807:1813	p < .05	1807:1813	p < .05	1807:1813	The APL exhibited higher (p < .05) antimicrobial potential than the APW.					
30007735	0	88	theme	lipid	95:99	arg1	oxidation					101:109	lipid oxidation	95:109	lipid oxidation	95:109	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	7	89	dep	glycogen	1060:1067	arg1	count					1131:1135	count	1131:1135	count	1131:1135	Diets had no effect (p > .05) on muscle glycogen, pH, drip loss, chemical composition and lactic acid bacteria count.					
30007735	1	90	theme	lumborum	358:365	arg1	muscle					373:378	Longissimus thoracis et lumborum (LTL) muscle	334:378	Longissimus thoracis et lumborum (LTL) muscle	334:378	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	13	91	theme	physicochemical	1891:1905	arg1	p < .05					1878:1884	p < .05	1878:1884	p < .05	1878:1884	Chill storage affected (p < .05) the physicochemical properties, lipid oxidation and microbial counts in chevon.					
30007735	13	91	theme	physicochemical	1891:1905	arg1	properties					1907:1916	the physicochemical properties	1887:1916	the physicochemical properties	1887:1916	Chill storage affected (p < .05) the physicochemical properties, lipid oxidation and microbial counts in chevon.					
30007735	4	92	theme	d	755:755	arg1	storage					763:769	a 7 d chill storage	751:769	a 7 d chill storage	751:769	The LTL muscle was subjected to a 7 d chill storage.					
30007735	2	93	theme	diet + 1.5	608:617	arg1	%					618:618	%	618:618	%	618:618	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	5	94	dep	higher	789:794	arg1	p < .05					797:803	p < .05	797:803	p < .05	797:803	The AP0 meat had higher (p < .05) concentration of C16:0 and C18:0 than the APW and APL meat.					
30007735	7	95	theme	chemical	1085:1092	arg1	composition					1094:1104	chemical composition	1085:1104	chemical composition	1085:1104	Diets had no effect (p > .05) on muscle glycogen, pH, drip loss, chemical composition and lactic acid bacteria count.					
30007735	2	96	theme	entire	417:422	arg1	four					411:414	four	411:414	four	411:414	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	96	theme	entire	417:422	arg1	4 months					436:443	4 months	436:443	4 months old	436:447	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	2	96	theme	entire	417:422	arg1	bucks					429:433	entire Boer bucks	417:433	entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW)	417:468	Twenty four, entire Boer bucks (4 months old; 20.18 ± 0.19 kg BW) were randomly allotted to either a basal diet without additive (AP0), a basal diet + 1.5% Andrographis paniculata leaves (APL) or a basal diet + 1.5% Andrographis paniculata whole plant (APW).					
30007735	1	97	theme	muscle	373:378	arg1	microbiota					297:306	microbiota	297:306	microbiota	297:306	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	97	theme	muscle	373:378	arg1	attributes					320:329	quality attributes	312:329	quality attributes	312:329	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	97	theme	muscle	373:378	arg1	acids					273:277	fatty acids	267:277	fatty acids	267:277	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	0	98	theme	different	27:35	arg1	parts					37:41	different parts	27:41	different parts of Andrographis paniculata	27:68	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	6	99	theme	total	906:910	arg1	CLA					912:914	total CLA	906:914	total CLA	906:914	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	7	100	dep	had	1026:1028	arg1	p > .05					1041:1047	p > .05	1041:1047	p > .05	1041:1047	Diets had no effect (p > .05) on muscle glycogen, pH, drip loss, chemical composition and lactic acid bacteria count.					
30007735	6	101	theme	C18:1trans	894:903	arg1	higher					964:969	higher	964:969	higher	964:969	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	6	101	theme	C18:1trans	894:903	arg1	concentrations					870:883	The concentrations	866:883	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3	866:957	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
30007735	8	102	theme	0.15 mg	1270:1276	arg1	MDA/kg					1278:1283	0.15 mg MDA/kg	1270:1283	0.15 mg MDA/kg	1270:1283	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	102	theme	0.15 mg	1270:1276	arg1	%					1258:1258	24.53%	1253:1258	24.53%	1253:1258	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	1	103	theme	different	221:229	arg1	parts					231:235	different parts	221:235	different parts of Andrographis paniculata	221:262	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	0	104	theme	paniculata	59:68	arg1	parts					37:41	different parts	27:41	different parts of Andrographis paniculata	27:68	Dietary supplementation of different parts of Andrographis paniculata affects the fatty acids, lipid oxidation, microbiota, and quality attributes of longissimus muscle in goats.					
30007735	10	105	dep	meat	1620:1623	arg1	5.41					1614:1617	5.41	1614:1617	5.41	1614:1617	Sensory scores for juiciness, tenderness, and overall acceptability of APL (7.92, 7.88, 7.89) and APW (7.90, 7.08, 7.77) meat were higher (p < .05) than that of the AP0 (5.38, 5.95, 5.41) meat.					
30007735	11	106	theme	spp	1677:1679	arg1	populations					1650:1660	populations	1650:1660	populations	1650:1660	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	11	106	theme	spp	1677:1679	arg1	counts					1639:1644	Total viable counts	1626:1644	Total viable counts	1626:1644	Total viable counts and populations of Pseudomonas spp, Escherichia coli and Enterobacteriacea were higher (p < .05) in AP0 meat than in APL and APW meat.					
30007735	4	107	theme	LTL	723:725	arg1	muscle					727:732	The LTL muscle	719:732	The LTL muscle	719:732	The LTL muscle was subjected to a 7 d chill storage.					
30007735	1	108	theme	quality	312:318	arg1	attributes					320:329	quality attributes	312:329	quality attributes	312:329	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	1	108	theme	quality	312:318	arg1	acids					273:277	fatty acids	267:277	fatty acids	267:277	The effects of dietary supplementation of different parts of Andrographis paniculata on fatty acids, lipid oxidation, microbiota and quality attributes of Longissimus thoracis et lumborum (LTL) muscle in goats were assessed.					
30007735	7	109	theme	muscle	1053:1058	arg1	glycogen					1060:1067	muscle glycogen	1053:1067	muscle glycogen	1053:1067	Diets had no effect (p > .05) on muscle glycogen, pH, drip loss, chemical composition and lactic acid bacteria count.					
30007735	7	110	contain	had	1026:1028	arg2	effect					1033:1038	no effect	1030:1038	no effect	1030:1038	Diets had no effect (p > .05) on muscle glycogen, pH, drip loss, chemical composition and lactic acid bacteria count.					
30007735	7	110	contain	had	1026:1028	arg1	Diets					1020:1024	Diets	1020:1024	Diets	1020:1024	Diets had no effect (p > .05) on muscle glycogen, pH, drip loss, chemical composition and lactic acid bacteria count.					
30007735	8	111	dep	APW	1248:1250	arg1	0.80 kg					1261:1267	0.80 kg	1261:1267	0.80 kg	1261:1267	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	111	dep	APW	1248:1250	arg1	MDA/kg					1278:1283	0.15 mg MDA/kg	1270:1283	0.15 mg MDA/kg	1270:1283	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	8	111	dep	APW	1248:1250	arg1	%					1258:1258	24.53%	1253:1258	24.53%	1253:1258	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	5	112	theme	APL	856:858	arg1	meat					860:863	APL meat	856:863	APL meat	856:863	The AP0 meat had higher (p < .05) concentration of C16:0 and C18:0 than the APW and APL meat.					
30007735	7	113	theme	lactic	1110:1115	arg1	bacteria					1122:1129	lactic acid bacteria	1110:1129	lactic acid bacteria	1110:1129	Diets had no effect (p > .05) on muscle glycogen, pH, drip loss, chemical composition and lactic acid bacteria count.					
30007735	8	114	theme	AP0	1305:1307	arg1	meat					1343:1346	AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat	1305:1346	AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat	1305:1346	Cooking loss, shear force, and TBARS values were lower (p < .05) in APL (23.98%, 0.76 kg, 0.12 mg MDA/kg) and APW (24.53%, 0.80 kg, 0.15 mg MDA/kg) meat compared with AP0 (26.49%, 1.13 kg, 0.23 mg MDA/kg) meat.					
30007735	6	115	dep	higher	964:969	arg1	p < .05					972:978	p < .05	972:978	p < .05	972:978	The concentrations of total C18:1trans, total CLA, C18:1n-9, C18:2n-6, C18:3n-3 and C20:5n-3 were higher (p < .05) in APL and APW meat than the AP0 meat.					
31663138	7	0	theme	%	1474:1474	arg1	composition					1476:1486	1% composition	1473:1486	1% composition	1473:1486	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	4	1	theme	protein	813:819	arg1	charge					821:826	protein charge and mass isoforms	813:844	charge	821:826	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	6	2	from	increase	1175:1182	arg1	ability					1191:1197	the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay	1187:1288	the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay	1187:1288	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	6	3	theme	dramatic	1166:1173	arg1	increase					1175:1182	a dramatic increase	1164:1182	a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay	1164:1288	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	2	4	theme	microfluidic	377:388	arg1	chip					390:393	an innovative microfluidic chip	363:393	an innovative microfluidic chip	363:393	Along with the construction of novel instrumentation and an innovative microfluidic chip, conversion to MS-compatible separation reagents has also been established.					
31663138	0	5	theme	charge	121:126	arg1	variants					128:135	biopharmaceutical charge variants	103:135	biopharmaceutical charge variants	103:135	A novel microchip-based imaged CIEF-MS system for comprehensive characterization and identification of biopharmaceutical charge variants.					
31663138	6	6	theme	quality	1239:1245	arg1	attributes					1247:1256	multiple intact mAb critical quality attributes	1210:1256	multiple intact mAb critical quality attributes	1210:1256	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	5	7	theme	isoforms	946:953	arg1	identification					894:907	identification	894:907	identification	894:907	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	5	7	theme	isoforms	946:953	arg1	Acquisition					863:873	Acquisition	863:873	Acquisition	863:873	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	5	7	theme	isoforms	946:953	arg1	quantitation					876:887	quantitation	876:887	quantitation	876:887	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	5	8	theme	analytical	1023:1032	arg1	utility					1034:1040	analytical utility	1023:1040	analytical utility of our innovative system	1023:1065	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	0	9	theme	biopharmaceutical	103:119	arg1	variants					128:135	biopharmaceutical charge variants	103:135	biopharmaceutical charge variants	103:135	A novel microchip-based imaged CIEF-MS system for comprehensive characterization and identification of biopharmaceutical charge variants.					
31663138	6	10	theme	mAb	1226:1228	arg1	attributes					1247:1256	multiple intact mAb critical quality attributes	1210:1256	multiple intact mAb critical quality attributes	1210:1256	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	4	11	theme	charge	821:826	arg1	identification					795:808	identification	795:808	identification	795:808	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	4	11	theme	charge	821:826	arg1	characterization					774:789	characterization	774:789	characterization	774:789	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	3	12	from	real-time	678:686	arg1	monitoring					627:636	the direct monitoring	616:636	the direct monitoring of analyte focusing and mobilization in real-time	616:686	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	1	13	theme	single	292:297	arg1	assay					299:303	a single assay	290:303	a single assay	290:303	A microfluidic system has been designed that integrates both imaged capillary isoelectric focusing (iCIEF) separations and downstream MS detection into a single assay.					
31663138	3	14	theme	photometric	541:551	arg1	quantitation					553:564	photometric quantitation	541:564	photometric quantitation of separated charge isoforms	541:593	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	7	15	theme	%	1387:1387	arg1	v/v					1389:1391	up to 4% v/v	1380:1391	up to 4% v/v	1380:1391	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	4	16	theme	isoforms	837:844	arg1	identification					795:808	identification	795:808	identification	795:808	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	4	16	theme	isoforms	837:844	arg1	characterization					774:789	characterization	774:789	characterization	774:789	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	2	17	theme	MS-compatible	410:422	arg1	reagents					435:442	MS-compatible separation reagents	410:442	MS-compatible separation reagents	410:442	Along with the construction of novel instrumentation and an innovative microfluidic chip, conversion to MS-compatible separation reagents has also been established.					
31663138	0	18	theme	variants	128:135	arg1	identification					85:98	identification	85:98	identification	85:98	A novel microchip-based imaged CIEF-MS system for comprehensive characterization and identification of biopharmaceutical charge variants.					
31663138	0	18	theme	variants	128:135	arg1	characterization					64:79	comprehensive characterization	50:79	comprehensive characterization	50:79	A novel microchip-based imaged CIEF-MS system for comprehensive characterization and identification of biopharmaceutical charge variants.					
31663138	6	19	theme	intact	1219:1224	arg1	attributes					1247:1256	multiple intact mAb critical quality attributes	1210:1256	multiple intact mAb critical quality attributes	1210:1256	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	4	20	theme	mass	832:835	arg1	isoforms					837:844	protein charge and mass isoforms	813:844	isoforms	837:844	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	1	21	theme	imaged	199:204	arg1	separations					245:255	imaged capillary isoelectric focusing (iCIEF) separations	199:255	imaged capillary isoelectric focusing (iCIEF) separations	199:255	A microfluidic system has been designed that integrates both imaged capillary isoelectric focusing (iCIEF) separations and downstream MS detection into a single assay.					
31663138	0	22	theme	novel	2:6	arg1	microchip-based					8:22	A novel microchip-based	0:22	A novel microchip-based	0:22	A novel microchip-based imaged CIEF-MS system for comprehensive characterization and identification of biopharmaceutical charge variants.					
31663138	6	23	theme	critical	1230:1237	arg1	attributes					1247:1256	multiple intact mAb critical quality attributes	1210:1256	multiple intact mAb critical quality attributes	1210:1256	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	2	24	theme	novel	337:341	arg1	instrumentation					343:357	novel instrumentation	337:357	novel instrumentation	337:357	Along with the construction of novel instrumentation and an innovative microfluidic chip, conversion to MS-compatible separation reagents has also been established.					
31663138	6	25	theme	iCIEF-MS	1135:1142	arg1	system					1144:1149	the iCIEF-MS system	1131:1149	the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay	1131:1288	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	1	26	theme	capillary	206:214	arg1	separations					245:255	imaged capillary isoelectric focusing (iCIEF) separations	199:255	imaged capillary isoelectric focusing (iCIEF) separations	199:255	A microfluidic system has been designed that integrates both imaged capillary isoelectric focusing (iCIEF) separations and downstream MS detection into a single assay.					
31663138	6	27	theme	separate	1081:1088	arg1	features					1100:1107	33 separate molecular features	1078:1107	33 separate molecular features	1078:1107	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	5	28	theme	resolved	919:926	arg1	isoforms					946:953	highly resolved intact mAb charge isoforms	912:953	highly resolved intact mAb charge isoforms	912:953	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	1	29	theme	isoelectric	216:226	arg1	separations					245:255	imaged capillary isoelectric focusing (iCIEF) separations	199:255	imaged capillary isoelectric focusing (iCIEF) separations	199:255	A microfluidic system has been designed that integrates both imaged capillary isoelectric focusing (iCIEF) separations and downstream MS detection into a single assay.					
31663138	7	30	dep	4	1386:1386	arg1	to					1383:1384	to	1383:1384	to	1383:1384	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	5	31	theme	glycan	990:995	arg1	pairs					997:1001	their critical N-linked glycan pairs	966:1001	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs	863:1001	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	3	32	theme	direct	620:625	arg1	monitoring					627:636	the direct monitoring	616:636	the direct monitoring of analyte focusing and mobilization in real-time	616:686	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	3	33	theme	analysis	515:522	arg1	Incorporation					471:483	Incorporation	471:483	Incorporation of 280 nm absorbance iCIEF-MS analysis	471:522	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	5	34	dep	along	955:959	arg1	with					961:964	with	961:964	with	961:964	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	7	35	theme	1	1473:1473	arg1	%					1474:1474	%	1474:1474	%	1474:1474	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	3	36	theme	separated	569:577	arg1	isoforms					586:593	separated charge isoforms	569:593	separated charge isoforms	569:593	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	3	37	from	mobilization	662:673	arg1	real-time					678:686	real-time	678:686	real-time	678:686	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	5	38	theme	charge	939:944	arg1	isoforms					946:953	highly resolved intact mAb charge isoforms	912:953	highly resolved intact mAb charge isoforms	912:953	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	5	39	theme	critical	972:979	arg1	pairs					997:1001	their critical N-linked glycan pairs	966:1001	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs	863:1001	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	3	40	theme	mobilization	662:673	arg1	monitoring					627:636	the direct monitoring	616:636	the direct monitoring of analyte focusing and mobilization in real-time	616:686	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	5	41	theme	mAb	935:937	arg1	isoforms					946:953	highly resolved intact mAb charge isoforms	912:953	highly resolved intact mAb charge isoforms	912:953	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	3	42	theme	absorbance	495:504	arg1	analysis					515:522	280 nm absorbance iCIEF-MS analysis	488:522	280 nm absorbance iCIEF-MS analysis	488:522	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	0	43	theme	CIEF-MS	31:37	arg1	system					39:44	CIEF-MS system	31:44	CIEF-MS system for comprehensive characterization and identification of biopharmaceutical charge variants	31:135	A novel microchip-based imaged CIEF-MS system for comprehensive characterization and identification of biopharmaceutical charge variants.					
31663138	5	44	theme	intact	928:933	arg1	isoforms					946:953	highly resolved intact mAb charge isoforms	912:953	highly resolved intact mAb charge isoforms	912:953	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	4	45	with	device	721:726	arg1	ability					744:750	the unique ability	733:750	the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min	733:860	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	3	46	theme	iCIEF-MS	506:513	arg1	analysis					515:522	280 nm absorbance iCIEF-MS analysis	488:522	280 nm absorbance iCIEF-MS analysis	488:522	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	0	47	theme	comprehensive	50:62	arg1	characterization					64:79	comprehensive characterization	50:79	comprehensive characterization	50:79	A novel microchip-based imaged CIEF-MS system for comprehensive characterization and identification of biopharmaceutical charge variants.					
31663138	5	48	link	N-linked	981:988	arg1	pairs					997:1001	their critical N-linked glycan pairs	966:1001	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs	863:1001	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	3	49	theme	focusing	649:656	arg1	monitoring					627:636	the direct monitoring	616:636	the direct monitoring of analyte focusing and mobilization in real-time	616:686	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	2	50	theme	innovative	366:375	arg1	chip					390:393	an innovative microfluidic chip	363:393	an innovative microfluidic chip	363:393	Along with the construction of novel instrumentation and an innovative microfluidic chip, conversion to MS-compatible separation reagents has also been established.					
31663138	4	51	dep	characterization	774:789	arg1	the					770:772	the	770:772	the	770:772	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	1	52	theme	microfluidic	140:151	arg1	system					153:158	A microfluidic system	138:158	A microfluidic system	138:158	A microfluidic system has been designed that integrates both imaged capillary isoelectric focusing (iCIEF) separations and downstream MS detection into a single assay.					
31663138	7	53	theme	high	1346:1349	arg1	concentrations					1361:1374	relatively high ampholyte concentrations	1335:1374	relatively high ampholyte concentrations	1335:1374	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	7	54	theme	MS	1426:1427	arg1	sensitivity					1429:1439	MS sensitivity	1426:1439	MS sensitivity	1426:1439	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	5	55	theme	system	1060:1065	arg1	utility					1034:1040	analytical utility	1023:1040	analytical utility of our innovative system	1023:1065	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	4	56	theme	unique	737:742	arg1	ability					744:750	the unique ability	733:750	the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min	733:860	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	5	57	theme	N-linked	981:988	arg1	pairs					997:1001	their critical N-linked glycan pairs	966:1001	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs	863:1001	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	3	58	from	monitoring	627:636	arg1	real-time					678:686	real-time	678:686	real-time	678:686	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	6	59	theme	molecular	1090:1098	arg1	features					1100:1107	33 separate molecular features	1078:1107	33 separate molecular features	1078:1107	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	5	60	theme	innovative	1049:1058	arg1	system					1060:1065	our innovative system	1045:1065	our innovative system	1045:1065	Acquisition, quantitation, and identification of highly resolved intact mAb charge isoforms along with their critical N-linked glycan pairs clearly demonstrate analytical utility of our innovative system.					
31663138	7	61	theme	composition	1476:1486	arg1	order					1464:1468	the order	1460:1468	the order of 1% composition	1460:1486	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	2	62	theme	instrumentation	343:357	arg1	construction					321:332	the construction	317:332	the construction of novel instrumentation	317:357	Along with the construction of novel instrumentation and an innovative microfluidic chip, conversion to MS-compatible separation reagents has also been established.					
31663138	2	62	theme	instrumentation	343:357	arg1	chip					390:393	an innovative microfluidic chip	363:393	an innovative microfluidic chip	363:393	Along with the construction of novel instrumentation and an innovative microfluidic chip, conversion to MS-compatible separation reagents has also been established.					
31663138	7	63	theme	v/v	1389:1391	arg1	concentrations					1361:1374	relatively high ampholyte concentrations	1335:1374	relatively high ampholyte concentrations	1335:1374	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	3	64	theme	280 nm	488:493	arg1	analysis					515:522	280 nm absorbance iCIEF-MS analysis	488:522	280 nm absorbance iCIEF-MS analysis	488:522	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	7	65	theme	reported	1309:1316	arg1	results					1326:1332	previously reported CIEF-MS results	1298:1332	previously reported CIEF-MS results	1298:1332	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	4	66	theme	effort	709:714	arg1	device					721:726	a device	719:726	a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min	719:860	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	4	66	theme	effort	709:714	arg1	outcome					693:699	The outcome	689:699	The outcome of this effort	689:714	The outcome of this effort is a device with the unique ability to allow for both the characterization and identification of protein charge and mass isoforms in under 15 min.					
31663138	6	67	theme	comprehensive	1270:1282	arg1	assay					1284:1288	a single comprehensive assay	1261:1288	a single comprehensive assay	1261:1288	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	3	68	theme	charge	579:584	arg1	isoforms					586:593	separated charge isoforms	569:593	separated charge isoforms	569:593	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	2	69	theme	separation	424:433	arg1	reagents					435:442	MS-compatible separation reagents	410:442	MS-compatible separation reagents	410:442	Along with the construction of novel instrumentation and an innovative microfluidic chip, conversion to MS-compatible separation reagents has also been established.					
31663138	1	70	theme	downstream	261:270	arg1	detection					275:283	downstream MS detection	261:283	downstream MS detection	261:283	A microfluidic system has been designed that integrates both imaged capillary isoelectric focusing (iCIEF) separations and downstream MS detection into a single assay.					
31663138	7	71	theme	CIEF-MS	1318:1324	arg1	results					1326:1332	previously reported CIEF-MS results	1298:1332	previously reported CIEF-MS results	1298:1332	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	6	72	theme	multiple	1210:1217	arg1	attributes					1247:1256	multiple intact mAb critical quality attributes	1210:1256	multiple intact mAb critical quality attributes	1210:1256	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	6	73	theme	single	1263:1268	arg1	assay					1284:1288	a single comprehensive assay	1261:1288	a single comprehensive assay	1261:1288	In total, 33 separate molecular features were characterized by the iCIEF-MS system representing a dramatic increase in the ability to monitor multiple intact mAb critical quality attributes in a single comprehensive assay.					
31663138	7	74	theme	ampholyte	1351:1359	arg1	concentrations					1361:1374	relatively high ampholyte concentrations	1335:1374	relatively high ampholyte concentrations	1335:1374	Unlike previously reported CIEF-MS results, relatively high ampholyte concentrations, of up to 4% v/v, were employed without impacting MS sensitivity, observed to be on the order of 1% composition.					
31663138	3	75	theme	isoforms	586:593	arg1	quantitation					553:564	photometric quantitation	541:564	photometric quantitation of separated charge isoforms	541:593	Incorporation of 280 nm absorbance iCIEF-MS analysis not only permits photometric quantitation of separated charge isoforms but also facilitates the direct monitoring of analyte focusing and mobilization in real-time.					
31663138	1	76	theme	MS	272:273	arg1	detection					275:283	downstream MS detection	261:283	downstream MS detection	261:283	A microfluidic system has been designed that integrates both imaged capillary isoelectric focusing (iCIEF) separations and downstream MS detection into a single assay.					
29384554	7	0	theme	molecular	1577:1585	arg1	weight					1587:1592	the highest average molecular weight	1557:1592	the highest average molecular weight	1557:1592	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	6	1	theme	insulin/l-penetratin	1166:1185	arg1	nanoparticles					1195:1207	insulin/l-penetratin complex nanoparticles	1166:1207	insulin/l-penetratin complex nanoparticles	1166:1207	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	6	1	theme	insulin/l-penetratin	1166:1185	arg1	core					1214:1217	a core	1212:1217	a core coated with hyaluronic acid (HA)	1212:1250	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	9	2	theme	oral	1993:1996	arg1	administration					1998:2011	a single oral administration	1984:2011	a single oral administration	1984:2011	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	5	3	theme	sequential	732:741	arg1	technique					772:780	a sequential flash nanocomplexation (FNC) technique	730:780	a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system	730:855	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	8	4	theme	rapid	1796:1800	arg1	release					1802:1808	rapid release	1796:1808	rapid release of insulin nanoparticles when they reach small intestine	1796:1865	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	7	5	theme	highest	1561:1567	arg1	weight					1587:1592	the highest average molecular weight	1557:1592	the highest average molecular weight	1557:1592	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	5	6	theme	nanocomplexation	749:764	arg1	technique					772:780	a sequential flash nanocomplexation (FNC) technique	730:780	a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system	730:855	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	4	7	theme	oral	481:484	arg1	delivery					486:493	nanoparticle-mediated oral delivery	459:493	nanoparticle-mediated oral delivery of protein therapeutics	459:517	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	7	8	theme	enhancement	1505:1515	arg1	effect					1517:1522	the enhancement effect	1501:1522	the enhancement effect	1501:1522	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	7	8	theme	enhancement	1505:1515	arg1	prominent					1533:1541	prominent	1533:1541	prominent	1533:1541	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	2	9	theme	scalable	314:321	arg1	fashion					323:329	a scalable fashion	312:329	a scalable fashion	312:329	Methods available for fabricating protein-encapsulating nanoparticles in a scalable fashion are scarce.					
29384554	8	10	theme	nanoparticles	1821:1833	arg1	release					1802:1808	rapid release	1796:1808	rapid release of insulin nanoparticles when they reach small intestine	1796:1865	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	5	11	dep	materials	870:878	arg1	materials					870:878	materials	870:878	materials choice and particle size and structure	870:917	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	5	11	dep	materials	870:878	arg1	choice					880:885	choice	880:885	choice	880:885	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	5	11	dep	materials	870:878	arg1	size					900:903	particle size	891:903	particle size	891:903	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	1	12	theme	clinical	197:204	arg1	translation					206:216	clinical translation	197:216	clinical translation of nanotherapeutics	197:236	Scalable manufacturing continues to present a major barrier for clinical translation of nanotherapeutics.					
29384554	6	13	theme	hyaluronic	1231:1240	arg1	acid					1242:1245	hyaluronic acid	1231:1245	hyaluronic acid (HA)	1231:1250	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	6	13	theme	hyaluronic	1231:1240	arg1	HA					1248:1249	HA	1248:1249	HA	1248:1249	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	0	14	theme	insulin	124:130	arg1	delivery					112:119	oral delivery	107:119	oral delivery of insulin	107:130	Scalable production of core-shell nanoparticles by flash nanocomplexation to enhance mucosal transport for oral delivery of insulin.					
29384554	8	15	theme	small	1851:1855	arg1	intestine					1857:1865	small intestine	1851:1865	small intestine	1851:1865	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	4	16	from	protection	520:529	arg1	release					558:564	site-specific release	544:564	site-specific release	544:564	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	4	16	from	protection	520:529	arg1	transport					630:638	enhancing epithelial transport	609:638	enhancing epithelial transport	609:638	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	4	16	from	protection	520:529	arg1	permeation					593:602	facilitating transmucosal permeation	567:602	facilitating transmucosal permeation	567:602	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	4	16	from	protection	520:529	arg1	tract					537:541	GI tract	534:541	GI tract	534:541	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	4	17	theme	GI	534:535	arg1	tract					537:541	GI tract	534:541	GI tract	534:541	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	7	18	theme	trans-epithelial	1443:1458	arg1	absorption					1460:1469	trans-epithelial absorption	1443:1469	trans-epithelial absorption of insulin nanoparticles	1443:1494	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	5	19	theme	FNC	767:769	arg1	technique					772:780	a sequential flash nanocomplexation (FNC) technique	730:780	a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system	730:855	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	8	20	from	microcapsules	1664:1676	arg1	process					1694:1700	an FNC process	1687:1700	an FNC process	1687:1700	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	4	21	theme	therapeutics	506:517	arg1	delivery					486:493	nanoparticle-mediated oral delivery	459:493	nanoparticle-mediated oral delivery of protein therapeutics	459:517	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	7	22	theme	mucus	1418:1422	arg1	layer					1424:1428	the intestinal mucus layer	1403:1428	the intestinal mucus layer	1403:1428	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	9	23	theme	free-form	2102:2110	arg1	insulin					2112:2118	free-form insulin	2102:2118	free-form insulin	2102:2118	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	10	24	theme	FNC	2126:2128	arg1	technique					2130:2138	This FNC technique	2121:2138	This FNC technique	2121:2138	This FNC technique is highly effective in controlling particle size and structure to improve delivery properties and function.					
29384554	5	25	theme	system	850:855	arg1	production					799:808	the scalable production	786:808	the scalable production of a core-shell structured nanoparticle system	786:855	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	2	26	theme	protein-encapsulating	273:293	arg1	nanoparticles					295:307	protein-encapsulating nanoparticles	273:307	protein-encapsulating nanoparticles	273:307	Methods available for fabricating protein-encapsulating nanoparticles in a scalable fashion are scarce.					
29384554	4	27	theme	facilitating	567:578	arg1	permeation					593:602	facilitating transmucosal permeation	567:602	facilitating transmucosal permeation	567:602	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	0	28	theme	mucosal	85:91	arg1	transport					93:101	mucosal transport	85:101	mucosal transport for oral delivery of insulin	85:130	Scalable production of core-shell nanoparticles by flash nanocomplexation to enhance mucosal transport for oral delivery of insulin.					
29384554	6	29	theme	high	1097:1100	arg1	%					1157:1157	67%	1155:1157	67%	1155:1157	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	6	29	theme	high	1097:1100	arg1	efficiency					1116:1125	high encapsulation efficiency	1097:1125	high encapsulation efficiency (97%)	1097:1131	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	6	29	theme	high	1097:1100	arg1	%					1130:1130	97%	1128:1130	97%	1128:1130	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	9	30	theme	multifunctional	1882:1896	arg1	MCs					1898:1900	The optimized multifunctional MCs	1868:1900	The optimized multifunctional MCs	1868:1900	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	9	31	with	comparison	2060:2069	arg1	injection					2089:2097	subcutaneous injection	2076:2097	subcutaneous injection of free-form insulin	2076:2118	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	8	32	from	environment	1754:1764	arg1	stomach					1773:1779	the stomach	1769:1779	the stomach	1769:1779	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	7	33	theme	in	1262:1263	arg1	models					1283:1288	Both the in vitro and in vivo models	1253:1288	models	1283:1288	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	7	34	theme	intestinal	1407:1416	arg1	layer					1424:1428	the intestinal mucus layer	1403:1428	the intestinal mucus layer	1403:1428	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	9	35	theme	effective	1915:1923	arg1	reduction					1933:1941	an effective glucose reduction	1912:1941	an effective glucose reduction in a Type I diabetes rat model	1912:1972	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	0	36	theme	Scalable	0:7	arg1	production					9:18	Scalable production	0:18	Scalable production of core-shell nanoparticles by flash nanocomplexation	0:72	Scalable production of core-shell nanoparticles by flash nanocomplexation to enhance mucosal transport for oral delivery of insulin.					
29384554	3	37	theme	same	428:431	arg1	vehicle					433:439	the same vehicle	424:439	the same vehicle	424:439	Protein delivery often requires multiple functionalities to be incorporated into the same vehicle.					
29384554	7	38	dep	in	1275:1276	arg1	vivo					1278:1281	vivo	1278:1281	vivo	1278:1281	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	7	39	from	coating	1312:1318	arg1	nanoparticles					1340:1352	these core-shell nanoparticles	1323:1352	these core-shell nanoparticles	1323:1352	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	6	40	theme	payload	1137:1143	arg1	capacity					1145:1152	payload capacity	1137:1152	payload capacity	1137:1152	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	4	41	theme	few	646:648	arg1	features					660:667	a few desirable features	644:667	a few desirable features	644:667	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	4	41	theme	few	646:648	arg1	protection					520:529	protection	520:529	protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport	520:638	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	7	42	dep	models	1283:1288	arg1	Both					1253:1256	Both	1253:1256	Both	1253:1256	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	0	43	theme	nanoparticles	34:46	arg1	production					9:18	Scalable production	0:18	Scalable production of core-shell nanoparticles by flash nanocomplexation	0:72	Scalable production of core-shell nanoparticles by flash nanocomplexation to enhance mucosal transport for oral delivery of insulin.					
29384554	11	44	theme	other	2295:2299	arg1	therapeutics					2309:2320	other protein therapeutics	2295:2320	other protein therapeutics	2295:2320	It can be easily extended to oral delivery for other protein therapeutics.					
29384554	5	45	theme	particle	891:898	arg1	materials					870:878	materials	870:878	materials choice and particle size and structure	870:917	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	5	45	theme	particle	891:898	arg1	size					900:903	particle size	891:903	particle size	891:903	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	9	46	theme	rat	1964:1966	arg1	model					1968:1972	a Type I diabetes rat model	1946:1972	a Type I diabetes rat model	1946:1972	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	4	47	theme	epithelial	619:628	arg1	transport					630:638	enhancing epithelial transport	609:638	enhancing epithelial transport	609:638	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	8	48	theme	acidic	1747:1752	arg1	environment					1754:1764	the acidic environment	1743:1764	the acidic environment in the stomach	1743:1779	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	8	49	theme	FNC	1690:1692	arg1	process					1694:1700	an FNC process	1687:1700	an FNC process	1687:1700	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	7	50	theme	average	1569:1575	arg1	weight					1587:1592	the highest average molecular weight	1557:1592	the highest average molecular weight	1557:1592	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	6	51	theme	complex	1187:1193	arg1	nanoparticles					1195:1207	insulin/l-penetratin complex nanoparticles	1166:1207	insulin/l-penetratin complex nanoparticles	1166:1207	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	6	51	theme	complex	1187:1193	arg1	core					1214:1217	a core	1212:1217	a core coated with hyaluronic acid (HA)	1212:1250	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	9	52	theme	single	1986:1991	arg1	administration					1998:2011	a single oral administration	1984:2011	a single oral administration	1984:2011	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	4	53	theme	nanoparticle	693:704	arg1	system					706:711	a nanoparticle system	691:711	a nanoparticle system	691:711	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	4	54	theme	nanoparticle-mediated	459:479	arg1	delivery					486:493	nanoparticle-mediated oral delivery	459:493	nanoparticle-mediated oral delivery of protein therapeutics	459:517	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	8	55	theme	additional	1713:1722	arg1	protection					1724:1733	additional protection	1713:1733	additional protection against the acidic environment in the stomach	1713:1779	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	5	56	theme	flash	743:747	arg1	technique					772:780	a sequential flash nanocomplexation (FNC) technique	730:780	a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system	730:855	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	9	57	from	reduction	1933:1941	arg1	model					1968:1972	a Type I diabetes rat model	1946:1972	a Type I diabetes rat model	1946:1972	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	1	58	theme	major	179:183	arg1	barrier					185:191	a major barrier	177:191	a major barrier for clinical translation of nanotherapeutics	177:236	Scalable manufacturing continues to present a major barrier for clinical translation of nanotherapeutics.					
29384554	0	59	theme	flash	51:55	arg1	nanocomplexation					57:72	flash nanocomplexation	51:72	flash nanocomplexation	51:72	Scalable production of core-shell nanoparticles by flash nanocomplexation to enhance mucosal transport for oral delivery of insulin.					
29384554	9	60	theme	relative	2025:2032	arg1	bioavailability					2034:2048	a relative bioavailability	2023:2048	a relative bioavailability of 11%	2023:2055	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	9	61	theme	optimized	1872:1880	arg1	MCs					1898:1900	The optimized multifunctional MCs	1868:1900	The optimized multifunctional MCs	1868:1900	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	8	62	theme	insulin	1813:1819	arg1	nanoparticles					1821:1833	insulin nanoparticles	1813:1833	insulin nanoparticles when they reach small intestine	1813:1865	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	6	63	theme	core-shell	1071:1080	arg1	structure					1082:1090	core-shell structure	1071:1090	core-shell structure	1071:1090	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	9	64	theme	%	2055:2055	arg1	bioavailability					2034:2048	a relative bioavailability	2023:2048	a relative bioavailability of 11%	2023:2055	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	5	65	theme	scalable	790:797	arg1	production					799:808	the scalable production	786:808	the scalable production of a core-shell structured nanoparticle system	786:855	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	5	66	theme	delivery	971:978	arg1	efficiency					980:989	the delivery efficiency	967:989	the delivery efficiency of insulin	967:1000	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	1	67	theme	nanotherapeutics	221:236	arg1	translation					206:216	clinical translation	197:216	clinical translation of nanotherapeutics	197:236	Scalable manufacturing continues to present a major barrier for clinical translation of nanotherapeutics.					
29384554	11	68	theme	protein	2301:2307	arg1	therapeutics					2309:2320	other protein therapeutics	2295:2320	other protein therapeutics	2295:2320	It can be easily extended to oral delivery for other protein therapeutics.					
29384554	7	69	theme	insulin	1474:1480	arg1	nanoparticles					1482:1494	insulin nanoparticles	1474:1494	insulin nanoparticles	1474:1494	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	2	70	theme	available	247:255	arg1	Methods					239:245	Methods	239:245	Methods available for fabricating protein-encapsulating nanoparticles in a scalable fashion	239:329	Methods available for fabricating protein-encapsulating nanoparticles in a scalable fashion are scarce.					
29384554	2	70	theme	available	247:255	arg1	scarce					335:340	scarce	335:340	scarce	335:340	Methods available for fabricating protein-encapsulating nanoparticles in a scalable fashion are scarce.					
29384554	4	71	theme	protein	498:504	arg1	therapeutics					506:517	protein therapeutics	498:517	protein therapeutics	498:517	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	9	72	theme	subcutaneous	2076:2087	arg1	injection					2089:2097	subcutaneous injection	2076:2097	subcutaneous injection of free-form insulin	2076:2118	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	1	73	theme	Scalable	133:140	arg1	manufacturing					142:154	Scalable manufacturing	133:154	Scalable manufacturing	133:154	Scalable manufacturing continues to present a major barrier for clinical translation of nanotherapeutics.					
29384554	7	74	theme	nanoparticles	1482:1494	arg1	absorption					1460:1469	trans-epithelial absorption	1443:1469	trans-epithelial absorption of insulin nanoparticles	1443:1494	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	3	75	theme	Protein	343:349	arg1	delivery					351:358	Protein delivery	343:358	Protein delivery	343:358	Protein delivery often requires multiple functionalities to be incorporated into the same vehicle.					
29384554	5	76	theme	nanoparticle	837:848	arg1	system					850:855	a core-shell structured nanoparticle system	813:855	a core-shell structured nanoparticle system	813:855	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	4	77	theme	transmucosal	580:591	arg1	permeation					593:602	facilitating transmucosal permeation	567:602	facilitating transmucosal permeation	567:602	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	9	78	theme	insulin	2112:2118	arg1	injection					2089:2097	subcutaneous injection	2076:2097	subcutaneous injection of free-form insulin	2076:2118	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	7	79	theme	in	1275:1276	arg1	models					1283:1288	Both the in vitro and in vivo models	1253:1288	models	1283:1288	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	5	80	theme	insulin	994:1000	arg1	efficiency					980:989	the delivery efficiency	967:989	the delivery efficiency of insulin	967:1000	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	6	81	theme	encapsulation	1102:1114	arg1	%					1157:1157	67%	1155:1157	67%	1155:1157	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	6	81	theme	encapsulation	1102:1114	arg1	efficiency					1116:1125	high encapsulation efficiency	1097:1125	high encapsulation efficiency (97%)	1097:1131	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	6	81	theme	encapsulation	1102:1114	arg1	%					1130:1130	97%	1128:1130	97%	1128:1130	This method is highly effective in controlling the size, generating core-shell structure with high encapsulation efficiency (97%) and payload capacity (67%) using insulin/l-penetratin complex nanoparticles as a core coated with hyaluronic acid (HA).					
29384554	3	82	theme	multiple	375:382	arg1	functionalities					384:398	multiple functionalities	375:398	multiple functionalities	375:398	Protein delivery often requires multiple functionalities to be incorporated into the same vehicle.					
29384554	8	83	theme	insulin-loaded	1599:1612	arg1	nanoparticles					1614:1626	The insulin-loaded nanoparticles	1595:1626	The insulin-loaded nanoparticles	1595:1626	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	0	84	theme	oral	107:110	arg1	delivery					112:119	oral delivery	107:119	oral delivery of insulin	107:130	Scalable production of core-shell nanoparticles by flash nanocomplexation to enhance mucosal transport for oral delivery of insulin.					
29384554	4	85	theme	site-specific	544:556	arg1	release					558:564	site-specific release	544:564	site-specific release	544:564	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	5	86	theme	core-shell	815:824	arg1	system					850:855	a core-shell structured nanoparticle system	813:855	a core-shell structured nanoparticle system	813:855	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	7	87	theme	nanoparticles	1381:1393	arg1	permeation					1367:1376	the permeation	1363:1376	the permeation of nanoparticles through the intestinal mucus layer	1363:1428	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	9	88	theme	glucose	1925:1931	arg1	reduction					1933:1941	an effective glucose reduction	1912:1941	an effective glucose reduction in a Type I diabetes rat model	1912:1972	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	8	89	theme	enteric	1656:1662	arg1	microcapsules					1664:1676	enteric microcapsules	1656:1676	enteric microcapsules (MCs) in an FNC process	1656:1700	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	8	89	theme	enteric	1656:1662	arg1	MCs					1679:1681	MCs	1679:1681	MCs	1679:1681	The insulin-loaded nanoparticles were then encapsulated into enteric microcapsules (MCs) in an FNC process to provide additional protection against the acidic environment in the stomach while allowing rapid release of insulin nanoparticles when they reach small intestine.					
29384554	0	90	theme	core-shell	23:32	arg1	nanoparticles					34:46	core-shell nanoparticles	23:46	core-shell nanoparticles	23:46	Scalable production of core-shell nanoparticles by flash nanocomplexation to enhance mucosal transport for oral delivery of insulin.					
29384554	11	91	theme	oral	2277:2280	arg1	delivery					2282:2289	oral delivery	2277:2289	oral delivery for other protein therapeutics	2277:2320	It can be easily extended to oral delivery for other protein therapeutics.					
29384554	4	92	theme	desirable	650:658	arg1	features					660:667	a few desirable features	644:667	a few desirable features	644:667	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	4	92	theme	desirable	650:658	arg1	protection					520:529	protection	520:529	protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport	520:638	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	9	93	theme	Type	1948:1951	arg1	model					1968:1972	a Type I diabetes rat model	1946:1972	a Type I diabetes rat model	1946:1972	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	4	94	theme	enhancing	609:617	arg1	transport					630:638	enhancing epithelial transport	609:638	enhancing epithelial transport	609:638	Specifically for nanoparticle-mediated oral delivery of protein therapeutics, protection in GI tract, site-specific release, facilitating transmucosal permeation, and enhancing epithelial transport are a few desirable features to be engineered into a nanoparticle system.					
29384554	5	95	theme	structured	826:835	arg1	system					850:855	a core-shell structured nanoparticle system	813:855	a core-shell structured nanoparticle system	813:855	Here we devised a sequential flash nanocomplexation (FNC) technique for the scalable production of a core-shell structured nanoparticle system by combining materials choice and particle size and structure to fulfill these functions, therefore enhancing the delivery efficiency of insulin.					
29384554	7	96	theme	core-shell	1329:1338	arg1	nanoparticles					1340:1352	these core-shell nanoparticles	1323:1352	these core-shell nanoparticles	1323:1352	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	9	97	theme	diabetes	1955:1962	arg1	model					1968:1972	a Type I diabetes rat model	1946:1972	a Type I diabetes rat model	1946:1972	The optimized multifunctional MCs delivered an effective glucose reduction in a Type I diabetes rat model following a single oral administration, yielding a relative bioavailability of 11% in comparison with subcutaneous injection of free-form insulin.					
29384554	7	98	dep	in	1262:1263	arg1	vitro					1265:1269	vitro	1265:1269	vitro	1265:1269	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	7	99	theme	HA	1309:1310	arg1	coating					1312:1318	the HA coating	1305:1318	the HA coating on these core-shell nanoparticles	1305:1352	Both the in vitro and in vivo models confirmed that the HA coating on these core-shell nanoparticles enhanced the permeation of nanoparticles through the intestinal mucus layer and improved trans-epithelial absorption of insulin nanoparticles; and the enhancement effect was most prominent using HA with the highest average molecular weight.					
29384554	10	100	theme	particle	2175:2182	arg1	size					2184:2187	particle size	2175:2187	particle size	2175:2187	This FNC technique is highly effective in controlling particle size and structure to improve delivery properties and function.					
29384554	10	101	theme	delivery	2214:2221	arg1	properties					2223:2232	delivery properties	2214:2232	delivery properties	2214:2232	This FNC technique is highly effective in controlling particle size and structure to improve delivery properties and function.					
30955591	1	0	theme	emulsifying	348:358	arg1	properties					372:381	emulsifying and foaming properties	348:381	emulsifying and foaming properties	348:381	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	0	theme	emulsifying	348:358	arg1	solubility					336:345	protein solubility	328:345	protein solubility	328:345	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	4	1	theme	protein	825:831	arg1	solubility					833:842	lower protein solubility	819:842	lower protein solubility	819:842	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	1	2	theme	acid	174:177	arg1	fermentation					179:190	lactic acid fermentation	167:190	lactic acid fermentation	167:190	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	0	3	theme	isolates	96:103	arg1	characterization					12:27	Biochemical characterization	0:27	Biochemical characterization	0:27	Biochemical characterization and technofunctional properties of bioprocessed wheat bran protein isolates.					
30955591	0	3	theme	isolates	96:103	arg1	properties					50:59	technofunctional properties	33:59	technofunctional properties	33:59	Biochemical characterization and technofunctional properties of bioprocessed wheat bran protein isolates.					
30955591	2	4	theme	starch	555:560	arg1	pentosans					574:582	starch and soluble pentosans	555:582	starch and soluble pentosans	555:582	The bioprocessing increased the protein (up to 80%) and fat content (up to 22.8%) in the isolates due to the degradation of starch and soluble pentosans.					
30955591	3	5	theme	globulin	606:613	arg1	proteins					596:603	Additional proteins	585:603	Additional proteins	585:603	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	3	5	theme	globulin	606:613	arg1	3A					615:616	globulin 3A	606:616	globulin 3A	606:616	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	1	6	theme	foaming	364:370	arg1	properties					372:381	emulsifying and foaming properties	348:381	emulsifying and foaming properties	348:381	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	6	theme	foaming	364:370	arg1	solubility					336:345	protein solubility	328:345	protein solubility	328:345	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	2	7	dep	80	478:479	arg1	to					475:476	to	475:476	to	475:476	The bioprocessing increased the protein (up to 80%) and fat content (up to 22.8%) in the isolates due to the degradation of starch and soluble pentosans.					
30955591	6	8	theme	cell	1131:1134	arg1	wall					1136:1139	cell wall	1131:1139	cell wall hydrolyzing enzymes and phytase	1131:1171	However, the foaming stability of the protein isolates was nearly doubled by bioprocessing with cell wall hydrolyzing enzymes and phytase.					
30955591	1	9	theme	carbohydrate	269:280	arg1	composition					282:292	carbohydrate composition	269:292	carbohydrate composition	269:292	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	9	theme	carbohydrate	269:280	arg1	protein					255:261	protein	255:261	protein	255:261	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	6	10	theme	isolates	1081:1088	arg1	stability					1056:1064	the foaming stability	1044:1064	the foaming stability of the protein isolates	1044:1088	However, the foaming stability of the protein isolates was nearly doubled by bioprocessing with cell wall hydrolyzing enzymes and phytase.					
30955591	4	11	theme	protein	799:805	arg1	isolate					807:813	the bioprocessed protein isolate	782:813	the bioprocessed protein isolate	782:813	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	1	12	dep	properties	316:325	arg1	properties					372:381	emulsifying and foaming properties	348:381	emulsifying and foaming properties	348:381	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	12	dep	properties	316:325	arg1	solubility					336:345	protein solubility	328:345	protein solubility	328:345	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	4	13	theme	bioprocessed	786:797	arg1	isolate					807:813	the bioprocessed protein isolate	782:813	the bioprocessed protein isolate	782:813	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	1	14	theme	cell	193:196	arg1	wall					198:201	cell wall	193:201	cell wall hydrolyzing enzymes	193:221	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	14	theme	cell	193:196	arg1	combinations					126:137	three combinations	120:137	three combinations of bioprocessing methods by lactic acid fermentation	120:190	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	15	theme	wheat	387:391	arg1	bran					393:396	wheat bran	387:396	wheat bran protein isolates	387:413	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	2	16	theme	pentosans	574:582	arg1	degradation					540:550	the degradation	536:550	the degradation of starch and soluble pentosans	536:582	The bioprocessing increased the protein (up to 80%) and fat content (up to 22.8%) in the isolates due to the degradation of starch and soluble pentosans.					
30955591	0	17	theme	Biochemical	0:10	arg1	characterization					12:27	Biochemical characterization	0:27	Biochemical characterization	0:27	Biochemical characterization and technofunctional properties of bioprocessed wheat bran protein isolates.					
30955591	3	18	theme	electrophoretic	691:705	arg1	pattern					707:713	the electrophoretic pattern	687:713	the electrophoretic pattern of the protein isolate bioprocessed with added enzymes	687:768	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	2	19	theme	soluble	566:572	arg1	pentosans					574:582	starch and soluble pentosans	555:582	starch and soluble pentosans	555:582	The bioprocessing increased the protein (up to 80%) and fat content (up to 22.8%) in the isolates due to the degradation of starch and soluble pentosans.					
30955591	4	20	theme	lower	819:823	arg1	solubility					833:842	lower protein solubility	819:842	lower protein solubility	819:842	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	0	21	theme	technofunctional	33:48	arg1	properties					50:59	technofunctional properties	33:59	technofunctional properties	33:59	Biochemical characterization and technofunctional properties of bioprocessed wheat bran protein isolates.					
30955591	5	22	theme	protein	982:988	arg1	isolates					990:997	the protein isolates	978:997	the protein isolates	978:997	The emulsifying properties of the protein isolates were not affected by bioprocessing.					
30955591	1	23	theme	bran	393:396	arg1	isolates					406:413	wheat bran protein isolates	387:413	wheat bran protein isolates	387:413	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	24	theme	technofunctional	299:314	arg1	properties					316:325	the biochemical (protein, fat, carbohydrate composition) and technofunctional properties	238:325	the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates	238:413	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	2	25	dep	22.8	506:509	arg1	to					503:504	to	503:504	to	503:504	The bioprocessing increased the protein (up to 80%) and fat content (up to 22.8%) in the isolates due to the degradation of starch and soluble pentosans.					
30955591	1	26	theme	protein	398:404	arg1	isolates					406:413	wheat bran protein isolates	387:413	wheat bran protein isolates	387:413	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	3	27	theme	protein	722:728	arg1	isolate					730:736	the protein isolate	718:736	the protein isolate bioprocessed with added enzymes	718:768	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	1	28	theme	isolates	406:413	arg1	properties					316:325	the biochemical (protein, fat, carbohydrate composition) and technofunctional properties	238:325	the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates	238:413	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	0	29	theme	wheat	77:81	arg1	isolates					96:103	bioprocessed wheat bran protein isolates	64:103	bioprocessed wheat bran protein isolates	64:103	Biochemical characterization and technofunctional properties of bioprocessed wheat bran protein isolates.					
30955591	3	30	theme	isolate	730:736	arg1	pattern					707:713	the electrophoretic pattern	687:713	the electrophoretic pattern of the protein isolate bioprocessed with added enzymes	687:768	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	4	31	theme	net	857:859	arg1	charge					861:866	stronger net charge	848:866	stronger net charge	848:866	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	1	32	dep	biochemical	242:252	arg1	fat					264:266	fat	264:266	fat	264:266	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	32	dep	biochemical	242:252	arg1	composition					282:292	carbohydrate composition	269:292	carbohydrate composition	269:292	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	32	dep	biochemical	242:252	arg1	protein					255:261	protein	255:261	protein	255:261	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	33	theme	combinations	126:137	arg1	effect					110:115	The effect	106:115	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates	106:413	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	0	34	theme	bioprocessed	64:75	arg1	isolates					96:103	bioprocessed wheat bran protein isolates	64:103	bioprocessed wheat bran protein isolates	64:103	Biochemical characterization and technofunctional properties of bioprocessed wheat bran protein isolates.					
30955591	1	35	theme	protein	328:334	arg1	properties					372:381	emulsifying and foaming properties	348:381	emulsifying and foaming properties	348:381	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	1	35	theme	protein	328:334	arg1	solubility					336:345	protein solubility	328:345	protein solubility	328:345	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	4	36	theme	stronger	848:855	arg1	charge					861:866	stronger net charge	848:866	stronger net charge	848:866	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	5	37	theme	isolates	990:997	arg1	properties					964:973	The emulsifying properties	948:973	The emulsifying properties of the protein isolates	948:997	The emulsifying properties of the protein isolates were not affected by bioprocessing.					
30955591	4	38	theme	protein	904:910	arg1	isolate					912:918	the protein isolate	900:918	the protein isolate made without bioprocessing	900:945	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	6	39	theme	protein	1073:1079	arg1	isolates					1081:1088	the protein isolates	1069:1088	the protein isolates	1069:1088	However, the foaming stability of the protein isolates was nearly doubled by bioprocessing with cell wall hydrolyzing enzymes and phytase.					
30955591	4	40	contain	had	815:817	arg1	isolate					807:813	the bioprocessed protein isolate	782:813	the bioprocessed protein isolate	782:813	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	4	40	contain	had	815:817	arg2	solubility					833:842	lower protein solubility	819:842	lower protein solubility	819:842	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	4	40	contain	had	815:817	arg2	charge					861:866	stronger net charge	848:866	stronger net charge	848:866	Generally, the bioprocessed protein isolate had lower protein solubility and stronger net charge in pH below 7, when compared to the protein isolate made without bioprocessing.					
30955591	3	41	theme	added	756:760	arg1	enzymes					762:768	added enzymes	756:768	added enzymes	756:768	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	3	42	theme	Additional	585:594	arg1	proteins					596:603	Additional proteins	585:603	Additional proteins	585:603	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	3	42	theme	Additional	585:594	arg1	3A					615:616	globulin 3A	606:616	globulin 3A	606:616	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	3	42	theme	Additional	585:594	arg1	β-amylase					637:645	β-amylase	637:645	β-amylase	637:645	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	3	42	theme	Additional	585:594	arg1	3C					622:623	3C	622:623	3C	622:623	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	3	42	theme	Additional	585:594	arg1	glutenins					655:663	LMW glutenins	651:663	LMW glutenins	651:663	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	3	42	theme	Additional	585:594	arg1	chitinase					626:634	chitinase	626:634	chitinase	626:634	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	2	43	theme	fat	487:489	arg1	%					510:510	up to 22.8%	500:510	up to 22.8%	500:510	The bioprocessing increased the protein (up to 80%) and fat content (up to 22.8%) in the isolates due to the degradation of starch and soluble pentosans.					
30955591	2	43	theme	fat	487:489	arg1	content					491:497	fat content	487:497	fat content (up to 22.8%)	487:511	The bioprocessing increased the protein (up to 80%) and fat content (up to 22.8%) in the isolates due to the degradation of starch and soluble pentosans.					
30955591	1	44	theme	biochemical	242:252	arg1	properties					316:325	the biochemical (protein, fat, carbohydrate composition) and technofunctional properties	238:325	the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates	238:413	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	0	45	theme	protein	88:94	arg1	isolates					96:103	bioprocessed wheat bran protein isolates	64:103	bioprocessed wheat bran protein isolates	64:103	Biochemical characterization and technofunctional properties of bioprocessed wheat bran protein isolates.					
30955591	6	46	theme	foaming	1048:1054	arg1	stability					1056:1064	the foaming stability	1044:1064	the foaming stability of the protein isolates	1044:1088	However, the foaming stability of the protein isolates was nearly doubled by bioprocessing with cell wall hydrolyzing enzymes and phytase.					
30955591	1	47	from	effect	110:115	arg1	properties					316:325	the biochemical (protein, fat, carbohydrate composition) and technofunctional properties	238:325	the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates	238:413	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
30955591	0	48	theme	bran	83:86	arg1	isolates					96:103	bioprocessed wheat bran protein isolates	64:103	bioprocessed wheat bran protein isolates	64:103	Biochemical characterization and technofunctional properties of bioprocessed wheat bran protein isolates.					
30955591	5	49	theme	emulsifying	952:962	arg1	properties					964:973	The emulsifying properties	948:973	The emulsifying properties of the protein isolates	948:997	The emulsifying properties of the protein isolates were not affected by bioprocessing.					
30955591	3	50	theme	LMW	651:653	arg1	proteins					596:603	Additional proteins	585:603	Additional proteins	585:603	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	3	50	theme	LMW	651:653	arg1	glutenins					655:663	LMW glutenins	651:663	LMW glutenins	651:663	Additional proteins, globulin 3A and 3C, chitinase, β-amylase and LMW glutenins, were identified from the electrophoretic pattern of the protein isolate bioprocessed with added enzymes.					
30955591	1	51	theme	lactic	167:172	arg1	acid					174:177	lactic acid	167:177	lactic acid fermentation	167:190	The effect of three combinations of bioprocessing methods by lactic acid fermentation, cell wall hydrolyzing enzymes and phytase on the biochemical (protein, fat, carbohydrate composition) and technofunctional properties (protein solubility, emulsifying and foaming properties) of wheat bran protein isolates were evaluated.					
31419555	4	0	theme	2-factor	494:501	arg1	design					543:548	2-factor, 3-level central composite experimental design	494:548	2-factor, 3-level central composite experimental design	494:548	The preparation method was optimized using 2-factor, 3-level central composite experimental design.					
31419555	6	1	theme	permeation	703:712	arg1	studies					714:720	ex vivo transcorneal permeation studies	682:720	ex vivo transcorneal permeation studies	682:720	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	1	2	from	characterization	75:90	arg1	treatment					124:132	glaucoma treatment	115:132	glaucoma treatment	115:132	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	4	3	theme	central	512:518	arg1	design					543:548	2-factor, 3-level central composite experimental design	494:548	2-factor, 3-level central composite experimental design	494:548	The preparation method was optimized using 2-factor, 3-level central composite experimental design.					
31419555	8	4	theme	nanoparticles	1056:1068	arg1	role					1026:1029	a promising role	1014:1029	a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment	1014:1115	Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.					
31419555	6	5	theme	DRZ	822:824	arg1	formulation					807:817	conventional formulation	794:817	conventional formulation of DRZ	794:824	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	8	6	theme	promising	1016:1024	arg1	role					1026:1029	a promising role	1014:1029	a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment	1014:1115	Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.					
31419555	7	7	theme	conventional	954:965	arg1	drops					971:975	conventional eye drops	954:975	conventional eye drops	954:975	The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops.					
31419555	3	8	theme	LLG	383:385	arg1	CMLLG					344:348	CMLLG	344:348	CMLLG	344:348	The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles.					
31419555	3	8	theme	LLG	383:385	arg1	carboxymethyl					329:341	The carboxymethyl	325:341	The carboxymethyl (CMLLG)	325:349	The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles.					
31419555	3	8	theme	LLG	383:385	arg1	derivative					361:370	amine derivative	355:370	amine derivative (AMLLG)	355:378	The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles.					
31419555	3	8	theme	LLG	383:385	arg1	AMLLG					373:377	AMLLG	373:377	AMLLG	373:377	The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles.					
31419555	1	9	from	Optimization	58:69	arg1	treatment					124:132	glaucoma treatment	115:132	glaucoma treatment	115:132	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	6	10	theme	higher	753:758	arg1	permeation					768:777	higher corneal permeation	753:777	higher corneal permeation	753:777	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	2	11	theme	galactomannan	304:316	arg1	leucocephala					291:302	derivatized Leucaena leucocephala galactomannan	270:316	derivatized Leucaena leucocephala galactomannan (LLG)	270:322	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	2	11	theme	galactomannan	304:316	arg1	LLG					319:321	LLG	319:321	LLG	319:321	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	6	12	theme	transcorneal	690:701	arg1	studies					714:720	ex vivo transcorneal permeation studies	682:720	ex vivo transcorneal permeation studies	682:720	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	8	13	theme	bioadhesive	1034:1044	arg1	nanoparticles					1056:1068	bioadhesive polymeric nanoparticles	1034:1068	bioadhesive polymeric nanoparticles	1034:1068	Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.					
31419555	6	14	theme	conventional	794:805	arg1	formulation					807:817	conventional formulation	794:817	conventional formulation of DRZ	794:824	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	3	15	theme	amine	355:359	arg1	AMLLG					373:377	AMLLG	373:377	AMLLG	373:377	The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles.					
31419555	3	15	theme	amine	355:359	arg1	derivative					361:370	amine derivative	355:370	amine derivative (AMLLG)	355:378	The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles.					
31419555	2	16	theme	present	142:148	arg1	study					150:154	the present study	138:154	the present study	138:154	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	6	17	dep	ex	682:683	arg1	vivo					685:688	vivo	685:688	vivo	685:688	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	2	18	theme	Leucaena	282:289	arg1	leucocephala					291:302	derivatized Leucaena leucocephala galactomannan	270:316	derivatized Leucaena leucocephala galactomannan (LLG)	270:322	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	2	18	theme	Leucaena	282:289	arg1	LLG					319:321	LLG	319:321	LLG	319:321	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	6	19	theme	ex	682:683	arg1	studies					714:720	ex vivo transcorneal permeation studies	682:720	ex vivo transcorneal permeation studies	682:720	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	5	20	theme	XRD	625:627	arg1	studies					629:635	XRD studies	625:635	XRD studies	625:635	The optimized nanoparticles were characterized by FTIR, DSC, SEM, TEM and XRD studies.					
31419555	7	21	theme	time	932:935	arg1	period					922:927	prolonged period	912:927	prolonged period of time	912:935	The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops.					
31419555	0	22	theme	Leucaena	0:7	arg1	Lam					23:25	Lam.	23:26	Lam.	23:26	Leucaena leucocephala (Lam.)					
31419555	0	22	theme	Leucaena	0:7	arg1	leucocephala					9:20	Leucaena leucocephala	0:20	Leucaena leucocephala (Lam.)	0:27	Leucaena leucocephala (Lam.)					
31419555	8	23	theme	polymeric	1046:1054	arg1	nanoparticles					1056:1068	bioadhesive polymeric nanoparticles	1034:1068	bioadhesive polymeric nanoparticles	1034:1068	Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.					
31419555	7	24	theme	prolonged	912:920	arg1	period					922:927	prolonged period	912:927	prolonged period of time	912:935	The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops.					
31419555	1	25	theme	ocular	96:101	arg1	delivery					103:110	ocular delivery	96:110	ocular delivery	96:110	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	6	26	theme	studies	670:676	arg1	results					642:648	The results	638:648	The results of in vitro release studies and ex vivo transcorneal permeation studies	638:720	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	7	27	theme	intra	880:884	arg1	IOP					903:905	IOP	903:905	IOP	903:905	The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops.					
31419555	7	27	theme	intra	880:884	arg1	pressure					893:900	the intra ocular pressure	876:900	the intra ocular pressure (IOP) for prolonged period of time	876:935	The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops.					
31419555	8	28	theme	ocular	1074:1079	arg1	delivery					1086:1093	ocular drug delivery	1074:1093	ocular drug delivery in glaucoma treatment	1074:1115	Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.					
31419555	7	29	theme	optimized	831:839	arg1	nanoparticles					841:853	The optimized nanoparticles	827:853	The optimized nanoparticles	827:853	The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops.					
31419555	6	30	theme	release	662:668	arg1	studies					670:676	in vitro release studies	653:676	in vitro release studies	653:676	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	1	31	dep	nanoparticles	43:55	arg1	characterization					75:90	characterization	75:90	characterization	75:90	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	1	31	dep	nanoparticles	43:55	arg1	Optimization					58:69	Optimization	58:69	Optimization	58:69	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	1	31	dep	nanoparticles	43:55	arg1	nanoparticles					43:55	galactomannan nanoparticles	29:55	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.	29:133	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	6	32	theme	in	653:654	arg1	studies					670:676	in vitro release studies	653:676	in vitro release studies	653:676	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	3	33	theme	nanoparticles	436:448	arg1	preparation					421:431	the preparation	417:431	the preparation of nanoparticles	417:448	The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles.					
31419555	4	34	theme	experimental	530:541	arg1	design					543:548	2-factor, 3-level central composite experimental design	494:548	2-factor, 3-level central composite experimental design	494:548	The preparation method was optimized using 2-factor, 3-level central composite experimental design.					
31419555	8	35	theme	drug	1081:1084	arg1	delivery					1086:1093	ocular drug delivery	1074:1093	ocular drug delivery in glaucoma treatment	1074:1115	Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.					
31419555	4	36	theme	preparation	455:465	arg1	method					467:472	The preparation method	451:472	The preparation method	451:472	The preparation method was optimized using 2-factor, 3-level central composite experimental design.					
31419555	8	37	theme	present	991:997	arg1	study					999:1003	the present study	987:1003	the present study	987:1003	Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.					
31419555	6	38	theme	corneal	760:766	arg1	permeation					768:777	higher corneal permeation	753:777	higher corneal permeation	753:777	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	4	39	theme	composite	520:528	arg1	design					543:548	2-factor, 3-level central composite experimental design	494:548	2-factor, 3-level central composite experimental design	494:548	The preparation method was optimized using 2-factor, 3-level central composite experimental design.					
31419555	8	40	theme	glaucoma	1098:1105	arg1	treatment					1107:1115	glaucoma treatment	1098:1115	glaucoma treatment	1098:1115	Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.					
31419555	2	41	theme	bioadhesive	157:167	arg1	nanoparticles					179:191	bioadhesive polymeric nanoparticles	157:191	bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ)	157:246	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	5	42	theme	optimized	555:563	arg1	nanoparticles					565:577	The optimized nanoparticles	551:577	The optimized nanoparticles	551:577	The optimized nanoparticles were characterized by FTIR, DSC, SEM, TEM and XRD studies.					
31419555	1	43	theme	glaucoma	115:122	arg1	treatment					124:132	glaucoma treatment	115:132	glaucoma treatment	115:132	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	2	44	theme	dorzolamide	216:226	arg1	DRZ					243:245	DRZ	243:245	DRZ	243:245	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	2	44	theme	dorzolamide	216:226	arg1	hydrochloride					228:240	dorzolamide hydrochloride	216:240	dorzolamide hydrochloride (DRZ)	216:246	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	2	45	theme	hydrochloride	228:240	arg1	delivery					204:211	ocular delivery	197:211	ocular delivery of dorzolamide hydrochloride (DRZ)	197:246	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	2	46	theme	polymeric	169:177	arg1	nanoparticles					179:191	bioadhesive polymeric nanoparticles	157:191	bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ)	157:246	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	4	47	dep	2-factor	494:501	arg1	3-level					504:510	3-level	504:510	3-level	504:510	The preparation method was optimized using 2-factor, 3-level central composite experimental design.					
31419555	3	48	used	used	408:411	arg2	carboxymethyl					329:341	The carboxymethyl	325:341	The carboxymethyl (CMLLG)	325:349	The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles.					
31419555	3	48	used	used	408:411	arg2	CMLLG					344:348	CMLLG	344:348	CMLLG	344:348	The carboxymethyl (CMLLG) and amine derivative (AMLLG) of LLG were synthesized and used for the preparation of nanoparticles.					
31419555	2	49	theme	derivatized	270:280	arg1	leucocephala					291:302	derivatized Leucaena leucocephala galactomannan	270:316	derivatized Leucaena leucocephala galactomannan (LLG)	270:322	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	2	49	theme	derivatized	270:280	arg1	LLG					319:321	LLG	319:321	LLG	319:321	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	6	50	dep	in	653:654	arg1	vitro					656:660	vitro	656:660	vitro	656:660	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	7	51	theme	eye	967:969	arg1	drops					971:975	conventional eye drops	954:975	conventional eye drops	954:975	The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops.					
31419555	8	52	from	delivery	1086:1093	arg1	treatment					1107:1115	glaucoma treatment	1098:1115	glaucoma treatment	1098:1115	Overall, the present study suggests a promising role of bioadhesive polymeric nanoparticles for ocular drug delivery in glaucoma treatment.					
31419555	6	53	theme	sustained	731:739	arg1	release					741:747	sustained release	731:747	sustained release	731:747	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	2	54	theme	ocular	197:202	arg1	delivery					204:211	ocular delivery	197:211	ocular delivery of dorzolamide hydrochloride (DRZ)	197:246	In the present study, bioadhesive polymeric nanoparticles for ocular delivery of dorzolamide hydrochloride (DRZ) were formulated using derivatized Leucaena leucocephala galactomannan (LLG).					
31419555	1	55	theme	galactomannan	29:41	arg1	characterization					75:90	characterization	75:90	characterization	75:90	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	1	55	theme	galactomannan	29:41	arg1	Optimization					58:69	Optimization	58:69	Optimization	58:69	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	1	55	theme	galactomannan	29:41	arg1	nanoparticles					43:55	galactomannan nanoparticles	29:55	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.	29:133	galactomannan nanoparticles: Optimization and characterization for ocular delivery in glaucoma treatment.					
31419555	6	56	theme	studies	714:720	arg1	results					642:648	The results	638:648	The results of in vitro release studies and ex vivo transcorneal permeation studies	638:720	The results of in vitro release studies and ex vivo transcorneal permeation studies revealed sustained release and higher corneal permeation as compared to conventional formulation of DRZ.					
31419555	7	57	theme	ocular	886:891	arg1	IOP					903:905	IOP	903:905	IOP	903:905	The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops.					
31419555	7	57	theme	ocular	886:891	arg1	pressure					893:900	the intra ocular pressure	876:900	the intra ocular pressure (IOP) for prolonged period of time	876:935	The optimized nanoparticles were found to reduce the intra ocular pressure (IOP) for prolonged period of time when compared to conventional eye drops.					
31469563	1	0	theme	intramolecular	190:203	arg1	transannulation					221:235	A rhodium-catalyzed intramolecular denitrogenative transannulation	170:235	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones	170:291	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
31469563	3	1	theme	N-linked	764:771	arg1	triazoles					773:781	N-linked triazoles	764:781	N-linked triazoles	764:781	In the case of O-linked triazoles, a cascade sequence consisting of intramolecular cyclopropanation and rearrangement takes place, leading to the formation of benzofurans, while, in the case of N-linked triazoles, cyclopropa[cd]indole-carbaldehydes were isolated exclusively.					
31469563	1	2	theme	denitrogenative	205:219	arg1	transannulation					221:235	A rhodium-catalyzed intramolecular denitrogenative transannulation	170:235	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones	170:291	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
31469563	1	3	theme	cyclopropa[cd	348:360	arg1	indole-carbaldehydes					362:381	benzofurans and cyclopropa[cd]indole-carbaldehydes	332:381	benzofurans and cyclopropa[cd]indole-carbaldehydes	332:381	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
31469563	3	4	theme	triazoles	773:781	arg1	case					756:759	the case	752:759	the case of N-linked triazoles	752:781	In the case of O-linked triazoles, a cascade sequence consisting of intramolecular cyclopropanation and rearrangement takes place, leading to the formation of benzofurans, while, in the case of N-linked triazoles, cyclopropa[cd]indole-carbaldehydes were isolated exclusively.					
31469563	3	5	theme	O-linked	585:592	arg1	triazoles					594:602	O-linked triazoles	585:602	O-linked triazoles	585:602	In the case of O-linked triazoles, a cascade sequence consisting of intramolecular cyclopropanation and rearrangement takes place, leading to the formation of benzofurans, while, in the case of N-linked triazoles, cyclopropa[cd]indole-carbaldehydes were isolated exclusively.					
31469563	1	6	theme	indole-carbaldehydes	362:381	arg1	synthesis					319:327	the synthesis	315:327	the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure	315:418	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
31469563	3	7	theme	cascade	607:613	arg1	sequence					615:622	a cascade sequence	605:622	a cascade sequence consisting of intramolecular cyclopropanation and rearrangement	605:686	In the case of O-linked triazoles, a cascade sequence consisting of intramolecular cyclopropanation and rearrangement takes place, leading to the formation of benzofurans, while, in the case of N-linked triazoles, cyclopropa[cd]indole-carbaldehydes were isolated exclusively.					
31469563	0	8	theme	Benzofurans	118:128	arg1	indole-carbaldehydes					148:167	Benzofurans and Cyclopropa[cd]indole-carbaldehydes	118:167	Benzofurans and Cyclopropa[cd]indole-carbaldehydes	118:167	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.					
31469563	0	9	theme	Rh	0:1	arg1	Transannulation					33:47	Rh(II)-Catalyzed Denitrogenative Transannulation	0:47	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.	0:168	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.					
31469563	1	10	theme	N-sulfonyl-1,2,3-triazole-tethered	240:273	arg1	cyclohexadienones					275:291	N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones	240:291	N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones	240:291	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
31469563	2	11	theme	cyclohexadienone	527:542	arg1	unit					544:547	the cyclohexadienone unit and triazole moiety	523:567	unit	544:547	Remarkably, the reaction pathway is fully dependent on the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety.					
31469563	3	12	link	N-linked	764:771	arg1	triazoles					773:781	N-linked triazoles	764:781	N-linked triazoles	764:781	In the case of O-linked triazoles, a cascade sequence consisting of intramolecular cyclopropanation and rearrangement takes place, leading to the formation of benzofurans, while, in the case of N-linked triazoles, cyclopropa[cd]indole-carbaldehydes were isolated exclusively.					
31469563	2	13	theme	linker	480:485	arg1	heteroatom					487:496	the linker heteroatom	476:496	the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety	476:567	Remarkably, the reaction pathway is fully dependent on the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety.					
31469563	3	14	link	O-linked	585:592	arg1	triazoles					594:602	O-linked triazoles	585:602	O-linked triazoles	585:602	In the case of O-linked triazoles, a cascade sequence consisting of intramolecular cyclopropanation and rearrangement takes place, leading to the formation of benzofurans, while, in the case of N-linked triazoles, cyclopropa[cd]indole-carbaldehydes were isolated exclusively.					
31469563	3	15	theme	intramolecular	638:651	arg1	cyclopropanation					653:668	intramolecular cyclopropanation	638:668	intramolecular cyclopropanation	638:668	In the case of O-linked triazoles, a cascade sequence consisting of intramolecular cyclopropanation and rearrangement takes place, leading to the formation of benzofurans, while, in the case of N-linked triazoles, cyclopropa[cd]indole-carbaldehydes were isolated exclusively.					
31469563	2	16	theme	present	507:513	arg1	heteroatom					487:496	the linker heteroatom	476:496	the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety	476:567	Remarkably, the reaction pathway is fully dependent on the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety.					
31469563	0	17	theme	Cyclopropa[cd	134:146	arg1	indole-carbaldehydes					148:167	Benzofurans and Cyclopropa[cd]indole-carbaldehydes	118:167	Benzofurans and Cyclopropa[cd]indole-carbaldehydes	118:167	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.					
31469563	3	18	theme	triazoles	594:602	arg1	case					577:580	the case	573:580	the case of O-linked triazoles	573:602	In the case of O-linked triazoles, a cascade sequence consisting of intramolecular cyclopropanation and rearrangement takes place, leading to the formation of benzofurans, while, in the case of N-linked triazoles, cyclopropa[cd]indole-carbaldehydes were isolated exclusively.					
31469563	0	19	theme	Denitrogenative	17:31	arg1	Transannulation					33:47	Rh(II)-Catalyzed Denitrogenative Transannulation	0:47	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.	0:168	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.					
31469563	1	20	theme	simple	403:408	arg1	procedure					410:418	an operationally simple procedure	386:418	an operationally simple procedure	386:418	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
31469563	1	21	theme	cyclohexadienones	275:291	arg1	transannulation					221:235	A rhodium-catalyzed intramolecular denitrogenative transannulation	170:235	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones	170:291	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
31469563	0	22	theme	-Catalyzed	6:15	arg1	Transannulation					33:47	Rh(II)-Catalyzed Denitrogenative Transannulation	0:47	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.	0:168	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.					
31469563	0	23	theme	indole-carbaldehydes	148:167	arg1	Synthesis					105:113	the Synthesis	101:113	the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes	101:167	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.					
31469563	3	24	theme	benzofurans	729:739	arg1	formation					716:724	the formation	712:724	the formation of benzofurans	712:739	In the case of O-linked triazoles, a cascade sequence consisting of intramolecular cyclopropanation and rearrangement takes place, leading to the formation of benzofurans, while, in the case of N-linked triazoles, cyclopropa[cd]indole-carbaldehydes were isolated exclusively.					
31469563	2	25	theme	triazole	553:560	arg1	moiety					562:567	the cyclohexadienone unit and triazole moiety	523:567	moiety	562:567	Remarkably, the reaction pathway is fully dependent on the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety.					
31469563	2	26	dep	heteroatom	487:496	arg1	N					504:504	N	504:504	N	504:504	Remarkably, the reaction pathway is fully dependent on the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety.					
31469563	2	26	dep	heteroatom	487:496	arg1	O					499:499	O	499:499	O	499:499	Remarkably, the reaction pathway is fully dependent on the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety.					
31469563	2	27	theme	reaction	437:444	arg1	pathway					446:452	the reaction pathway	433:452	the reaction pathway	433:452	Remarkably, the reaction pathway is fully dependent on the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety.					
31469563	2	27	theme	reaction	437:444	arg1	dependent					463:471	dependent	463:471	dependent	463:471	Remarkably, the reaction pathway is fully dependent on the linker heteroatom (O or N) present between the cyclohexadienone unit and triazole moiety.					
31469563	1	28	from	synthesis	319:327	arg1	procedure					410:418	an operationally simple procedure	386:418	an operationally simple procedure	386:418	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
31469563	0	29	theme	Cyclohexadienones	79:95	arg1	Transannulation					33:47	Rh(II)-Catalyzed Denitrogenative Transannulation	0:47	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.	0:168	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.					
31469563	0	30	theme	N-Sulfonyl-1,2,3-triazolyl	52:77	arg1	Cyclohexadienones					79:95	N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones	52:95	N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones	52:95	Rh(II)-Catalyzed Denitrogenative Transannulation of N-Sulfonyl-1,2,3-triazolyl Cyclohexadienones for the Synthesis of Benzofurans and Cyclopropa[cd]indole-carbaldehydes.					
31469563	1	31	theme	rhodium-catalyzed	172:188	arg1	transannulation					221:235	A rhodium-catalyzed intramolecular denitrogenative transannulation	170:235	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones	170:291	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
31469563	1	32	theme	benzofurans	332:342	arg1	indole-carbaldehydes					362:381	benzofurans and cyclopropa[cd]indole-carbaldehydes	332:381	benzofurans and cyclopropa[cd]indole-carbaldehydes	332:381	A rhodium-catalyzed intramolecular denitrogenative transannulation of N-sulfonyl-1,2,3-triazole-tethered cyclohexadienones has been achieved for the synthesis of benzofurans and cyclopropa[cd]indole-carbaldehydes in an operationally simple procedure.					
30415857	3	0	theme	stover	570:575	arg1	silage					577:582	sticky corn stover silage	558:582	sticky corn stover silage	558:582	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	0	1	with	silage	54:59	arg1	anthocyanins					75:86	abundant anthocyanins	66:86	abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats	66:194	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	1	2	theme	Saanen	245:250	arg1	weight					270:275	body weight	265:275	body weight	265:275	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	1	2	theme	Saanen	245:250	arg1	goats					258:262	16 multiparous lactating Saanen dairy goats	220:262	16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders	220:356	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	11	3	theme	positive	1756:1763	arg1	correlations					1765:1776	stronger positive correlations	1747:1776	stronger positive correlations	1747:1776	Specifically, stronger positive correlations were noted between several antioxidant enzymes and anthocyanin composition in milk (total antioxidant capacity and Pel; SOD and Peo as well as SOD and M3G).					
30415857	11	4	theme	several	1797:1803	arg1	enzymes					1817:1823	several antioxidant enzymes	1797:1823	several antioxidant enzymes	1797:1823	Specifically, stronger positive correlations were noted between several antioxidant enzymes and anthocyanin composition in milk (total antioxidant capacity and Pel; SOD and Peo as well as SOD and M3G).					
30415857	0	5	theme	lactating	174:182	arg1	goats					190:194	lactating dairy goats	174:194	lactating dairy goats	174:194	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	0	6	theme	goats	190:194	arg1	status					164:169	antioxidant status	152:169	antioxidant status of lactating dairy goats	152:194	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	11	7	theme	anthocyanin	1829:1839	arg1	composition					1841:1851	anthocyanin composition	1829:1851	anthocyanin composition	1829:1851	Specifically, stronger positive correlations were noted between several antioxidant enzymes and anthocyanin composition in milk (total antioxidant capacity and Pel; SOD and Peo as well as SOD and M3G).					
30415857	10	8	located	observed	1603:1610	arg2	correlations					1585:1596	the positive correlations	1572:1596	the positive correlations	1572:1596	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	8	located	observed	1603:1610	arg1	addition					1562:1569	addition	1562:1569	addition	1562:1569	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	2	9	theme	block	476:480	arg1	design					482:487	a randomized completed block design	453:487	a randomized completed block design	453:487	Goats were divided into 2 blocks of 8 goats based on milk yield averaged from 75 d in milk in a randomized completed block design.					
30415857	5	10	from	anthocyanins	997:1008	arg1	milk					1013:1016	milk	1013:1016	milk	1013:1016	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	4	11	from	elevation	751:759	arg1	content					768:774	the content	764:774	the content of milk lactose relative to the CSSS	764:811	The results indicated that the TPSS group led to an elevation in the content of milk lactose relative to the CSSS.					
30415857	7	12	dep	resulted	1123:1130	arg1	compared					1200:1207	compared	1200:1207	compared with the control	1200:1224	However, the TPSS resulted in higher levels of peonidin (Peo) and malvidin-3-O-glucoside (M3G) compared with the control.					
30415857	2	13	theme	randomized	455:464	arg1	design					482:487	a randomized completed block design	453:487	a randomized completed block design	453:487	Goats were divided into 2 blocks of 8 goats based on milk yield averaged from 75 d in milk in a randomized completed block design.					
30415857	7	14	theme	peonidin	1152:1159	arg1	levels					1142:1147	higher levels	1135:1147	higher levels of peonidin (Peo) and malvidin-3-O-glucoside (M3G)	1135:1198	However, the TPSS resulted in higher levels of peonidin (Peo) and malvidin-3-O-glucoside (M3G) compared with the control.					
30415857	3	15	dep	fed	554:556	arg1	fed					631:633	fed	631:633	were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS)	626:696	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	1	16	theme	present	201:207	arg1	study					209:213	The present study	197:213	The present study	197:213	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	9	17	theme	total	1461:1465	arg1	solids					1467:1472	total solids	1461:1472	total solids	1461:1472	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	5	18	from	cyanidin	950:957	arg1	milk					1013:1016	milk	1013:1016	milk	1013:1016	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	2	19	from	d	440:440	arg1	milk					445:448	milk	445:448	milk	445:448	Goats were divided into 2 blocks of 8 goats based on milk yield averaged from 75 d in milk in a randomized completed block design.					
30415857	2	19	from	d	440:440	arg1	design					482:487	a randomized completed block design	453:487	a randomized completed block design	453:487	Goats were divided into 2 blocks of 8 goats based on milk yield averaged from 75 d in milk in a randomized completed block design.					
30415857	6	20	located	detected	1080:1087	arg2	unable					1067:1072	unable	1067:1072	unable	1067:1072	The pelargonidin-3-glucoside and malvidin were unable to be detected in both groups.					
30415857	6	20	located	detected	1080:1087	arg1	groups					1097:1102	both groups	1092:1102	both groups	1092:1102	The pelargonidin-3-glucoside and malvidin were unable to be detected in both groups.					
30415857	6	20	located	detected	1080:1087	arg2	malvidin					1053:1060	malvidin	1053:1060	malvidin	1053:1060	The pelargonidin-3-glucoside and malvidin were unable to be detected in both groups.					
30415857	6	20	located	detected	1080:1087	arg2	pelargonidin-3-glucoside					1024:1047	pelargonidin-3-glucoside	1024:1047	pelargonidin-3-glucoside	1024:1047	The pelargonidin-3-glucoside and malvidin were unable to be detected in both groups.					
30415857	4	21	theme	lactose	784:790	arg1	content					768:774	the content	764:774	the content of milk lactose relative to the CSSS	764:811	The results indicated that the TPSS group led to an elevation in the content of milk lactose relative to the CSSS.					
30415857	3	22	theme	purple	652:657	arg1	L.					674:675	Zea mays L.	665:675	Zea mays L.	665:675	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	22	theme	purple	652:657	arg1	corn					659:662	anthocyanin-rich purple corn	635:662	anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS)	635:696	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	5	23	theme	total	991:995	arg1	anthocyanins					997:1008	total anthocyanins	991:1008	total anthocyanins in milk	991:1016	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	1	24	theme	±	300:300	arg1	deviation					311:319	mean ± standard deviation	295:319	mean ± standard deviation	295:319	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	10	25	theme	total	1713:1717	arg1	anthocyanins					1719:1730	total anthocyanins	1713:1730	total anthocyanins	1713:1730	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	5	26	theme	particular	884:893	arg1	delphinidin					937:947	delphinidin	937:947	delphinidin	937:947	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	26	theme	particular	884:893	arg1	anthocyanins					895:906	5 particular anthocyanins	882:906	5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk]	882:1017	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	26	theme	particular	884:893	arg1	[i.e.					908:912	[i.e.	908:912	[i.e.	908:912	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	26	theme	particular	884:893	arg1	cyanidin					950:957	cyanidin	950:957	cyanidin	950:957	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	26	theme	particular	884:893	arg1	pelargonidin					960:971	pelargonidin	960:971	pelargonidin (Pel)	960:977	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	26	theme	particular	884:893	arg1	cyanidin-3-glucoside					915:934	cyanidin-3-glucoside	915:934	cyanidin-3-glucoside	915:934	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	0	27	dep	anthocyanins	75:86	arg1	increasing					141:150	increasing	141:150	increasing antioxidant status of lactating dairy goats	141:194	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	0	27	dep	anthocyanins	75:86	arg1	transferring					88:99	transferring	88:99	transferring anthocyanin composition to the milk	88:135	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	10	28	theme	positive	1576:1583	arg1	correlations					1585:1596	the positive correlations	1572:1596	the positive correlations	1572:1596	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	11	29	theme	antioxidant	1868:1878	arg1	capacity					1880:1887	total antioxidant capacity	1862:1887	total antioxidant capacity	1862:1887	Specifically, stronger positive correlations were noted between several antioxidant enzymes and anthocyanin composition in milk (total antioxidant capacity and Pel; SOD and Peo as well as SOD and M3G).					
30415857	8	30	theme	superoxide	1286:1295	arg1	SOD					1308:1310	SOD	1308:1310	SOD	1308:1310	Moreover, goats receiving TPSS exhibited a higher level of superoxide dismutase (SOD) in plasma and milk relative to the CSSS.					
30415857	8	30	theme	superoxide	1286:1295	arg1	dismutase					1297:1305	superoxide dismutase	1286:1305	superoxide dismutase (SOD)	1286:1311	Moreover, goats receiving TPSS exhibited a higher level of superoxide dismutase (SOD) in plasma and milk relative to the CSSS.					
30415857	0	31	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.	0:195	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	2	32	theme	milk	412:415	arg1	yield					417:421	milk yield	412:421	milk yield averaged from 75 d in milk in a randomized completed block design	412:487	Goats were divided into 2 blocks of 8 goats based on milk yield averaged from 75 d in milk in a randomized completed block design.					
30415857	9	33	dep	components	1435:1444	arg1	solids					1467:1472	total solids	1461:1472	total solids	1461:1472	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	33	dep	components	1435:1444	arg1	SNF					1509:1511	SNF	1509:1511	SNF	1509:1511	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	33	dep	components	1435:1444	arg1	[i.e.					1446:1450	[i.e.	1446:1450	[i.e.	1446:1450	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	33	dep	components	1435:1444	arg1	fat					1453:1455	fat	1453:1455	fat	1453:1455	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	33	dep	components	1435:1444	arg1	fat					1485:1487	fat	1485:1487	fat	1485:1487	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	33	dep	components	1435:1444	arg1	components					1435:1444	the certain milk components	1418:1444	the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF)	1418:1512	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	0	34	theme	Zea	34:36	arg1	L.					43:44	Zea mays L.	34:44	Zea mays L.	34:44	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	0	34	theme	Zea	34:36	arg1	corn					28:31	Purple corn	21:31	Purple corn (Zea mays L.)	21:45	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	5	35	theme	PSS	848:850	arg1	inclusion					818:826	The inclusion	814:826	The inclusion of anthocyanin-rich PSS	814:850	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	36	dep	anthocyanins	895:906	arg1	anthocyanins					997:1008	total anthocyanins	991:1008	total anthocyanins in milk	991:1016	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	36	dep	anthocyanins	895:906	arg1	delphinidin					937:947	delphinidin	937:947	delphinidin	937:947	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	36	dep	anthocyanins	895:906	arg1	Pel					974:976	Pel	974:976	Pel	974:976	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	36	dep	anthocyanins	895:906	arg1	anthocyanins					895:906	5 particular anthocyanins	882:906	5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk]	882:1017	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	36	dep	anthocyanins	895:906	arg1	[i.e.					908:912	[i.e.	908:912	[i.e.	908:912	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	36	dep	anthocyanins	895:906	arg1	cyanidin					950:957	cyanidin	950:957	cyanidin	950:957	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	36	dep	anthocyanins	895:906	arg1	pelargonidin					960:971	pelargonidin	960:971	pelargonidin (Pel)	960:977	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	36	dep	anthocyanins	895:906	arg1	cyanidin-3-glucoside					915:934	cyanidin-3-glucoside	915:934	cyanidin-3-glucoside	915:934	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	3	37	theme	corn	565:568	arg1	silage					577:582	sticky corn stover silage	558:582	sticky corn stover silage	558:582	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	1	38	used	used	215:218	arg2	study					209:213	The present study	197:213	The present study	197:213	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	12	39	theme	antioxidants	2046:2057	arg1	amount					2036:2041	the amount	2032:2041	the amount of antioxidants	2032:2057	Taken together, PSS with abundant anthocyanins can transfer anthocyanins to the milk and enhance the amount of antioxidants in lactating dairy goats.					
30415857	12	39	theme	antioxidants	2046:2057	arg1	antioxidants					2046:2057	antioxidants	2046:2057	antioxidants	2046:2057	Taken together, PSS with abundant anthocyanins can transfer anthocyanins to the milk and enhance the amount of antioxidants in lactating dairy goats.					
30415857	5	40	from	cyanidin-3-glucoside	915:934	arg1	milk					1013:1016	milk	1013:1016	milk	1013:1016	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	9	41	theme	positive	1374:1381	arg1	correlations					1383:1394	some positive correlations	1369:1394	some positive correlations	1369:1394	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	12	42	theme	lactating	2062:2070	arg1	goats					2078:2082	lactating dairy goats	2062:2082	lactating dairy goats	2062:2082	Taken together, PSS with abundant anthocyanins can transfer anthocyanins to the milk and enhance the amount of antioxidants in lactating dairy goats.					
30415857	5	43	from	pelargonidin	960:971	arg1	milk					1013:1016	milk	1013:1016	milk	1013:1016	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	1	44	theme	lactating	235:243	arg1	weight					270:275	body weight	265:275	body weight	265:275	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	1	44	theme	lactating	235:243	arg1	goats					258:262	16 multiparous lactating Saanen dairy goats	220:262	16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders	220:356	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	9	45	theme	milk	1430:1433	arg1	fat					1453:1455	fat	1453:1455	fat	1453:1455	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	45	theme	milk	1430:1433	arg1	[i.e.					1446:1450	[i.e.	1446:1450	[i.e.	1446:1450	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	45	theme	milk	1430:1433	arg1	solids-not-fat					1493:1506	solids-not-fat	1493:1506	solids-not-fat	1493:1506	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	45	theme	milk	1430:1433	arg1	fat					1485:1487	fat	1485:1487	fat	1485:1487	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	45	theme	milk	1430:1433	arg1	components					1435:1444	the certain milk components	1418:1444	the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF)	1418:1512	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	0	46	theme	dairy	184:188	arg1	goats					190:194	lactating dairy goats	174:194	lactating dairy goats	174:194	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	1	47	theme	dairy	252:256	arg1	weight					270:275	body weight	265:275	body weight	265:275	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	1	47	theme	dairy	252:256	arg1	goats					258:262	16 multiparous lactating Saanen dairy goats	220:262	16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders	220:356	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	11	48	theme	antioxidant	1805:1815	arg1	enzymes					1817:1823	several antioxidant enzymes	1797:1823	several antioxidant enzymes	1797:1823	Specifically, stronger positive correlations were noted between several antioxidant enzymes and anthocyanin composition in milk (total antioxidant capacity and Pel; SOD and Peo as well as SOD and M3G).					
30415857	2	49	theme	goats	397:401	arg1	blocks					385:390	2 blocks	383:390	2 blocks of 8 goats based on milk yield averaged from 75 d in milk in a randomized completed block design	383:487	Goats were divided into 2 blocks of 8 goats based on milk yield averaged from 75 d in milk in a randomized completed block design.					
30415857	9	50	theme	certain	1422:1428	arg1	fat					1453:1455	fat	1453:1455	fat	1453:1455	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	50	theme	certain	1422:1428	arg1	[i.e.					1446:1450	[i.e.	1446:1450	[i.e.	1446:1450	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	50	theme	certain	1422:1428	arg1	solids-not-fat					1493:1506	solids-not-fat	1493:1506	solids-not-fat	1493:1506	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	50	theme	certain	1422:1428	arg1	fat					1485:1487	fat	1485:1487	fat	1485:1487	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	9	50	theme	certain	1422:1428	arg1	components					1435:1444	the certain milk components	1418:1444	the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF)	1418:1512	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	10	51	theme	individual	1620:1629	arg1	Pel					1704:1706	Pel	1704:1706	Pel	1704:1706	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	51	theme	individual	1620:1629	arg1	anthocyanins					1631:1642	individual anthocyanins	1620:1642	individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel)	1620:1707	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	51	theme	individual	1620:1629	arg1	cyanidin-3-glucoside					1645:1664	cyanidin-3-glucoside	1645:1664	cyanidin-3-glucoside	1645:1664	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	51	theme	individual	1620:1629	arg1	M3G					1685:1687	M3G	1685:1687	M3G	1685:1687	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	51	theme	individual	1620:1629	arg1	cyanidin					1690:1697	cyanidin	1690:1697	cyanidin	1690:1697	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	51	theme	individual	1620:1629	arg1	delphinidin					1667:1677	delphinidin	1667:1677	delphinidin	1667:1677	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	51	theme	individual	1620:1629	arg1	Peo					1680:1682	Peo	1680:1682	Peo	1680:1682	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	12	52	theme	abundant	1960:1967	arg1	anthocyanins					1969:1980	abundant anthocyanins	1960:1980	abundant anthocyanins	1960:1980	Taken together, PSS with abundant anthocyanins can transfer anthocyanins to the milk and enhance the amount of antioxidants in lactating dairy goats.					
30415857	7	53	theme	malvidin-3-O-glucoside	1171:1192	arg1	levels					1142:1147	higher levels	1135:1147	higher levels of peonidin (Peo) and malvidin-3-O-glucoside (M3G)	1135:1198	However, the TPSS resulted in higher levels of peonidin (Peo) and malvidin-3-O-glucoside (M3G) compared with the control.					
30415857	12	54	with	PSS	1951:1953	arg1	anthocyanins					1969:1980	abundant anthocyanins	1960:1980	abundant anthocyanins	1960:1980	Taken together, PSS with abundant anthocyanins can transfer anthocyanins to the milk and enhance the amount of antioxidants in lactating dairy goats.					
30415857	2	55	theme	completed	466:474	arg1	design					482:487	a randomized completed block design	453:487	a randomized completed block design	453:487	Goats were divided into 2 blocks of 8 goats based on milk yield averaged from 75 d in milk in a randomized completed block design.					
30415857	1	56	theme	symmetrical	339:349	arg1	udders					351:356	healthy and symmetrical udders	327:356	healthy and symmetrical udders	327:356	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	3	57	theme	anthocyanin-rich	635:650	arg1	L.					674:675	Zea mays L.	665:675	Zea mays L.	665:675	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	57	theme	anthocyanin-rich	635:650	arg1	corn					659:662	anthocyanin-rich purple corn	635:662	anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS)	635:696	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	58	theme	study	496:500	arg1	CSSS					527:530	the control (CSSS)	514:531	the control (CSSS)	514:531	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	58	theme	study	496:500	arg1	groups					502:507	The 2 study groups	490:507	The 2 study groups	490:507	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	11	59	theme	stronger	1747:1754	arg1	correlations					1765:1776	stronger positive correlations	1747:1776	stronger positive correlations	1747:1776	Specifically, stronger positive correlations were noted between several antioxidant enzymes and anthocyanin composition in milk (total antioxidant capacity and Pel; SOD and Peo as well as SOD and M3G).					
30415857	0	60	theme	antioxidant	152:162	arg1	status					164:169	antioxidant status	152:169	antioxidant status of lactating dairy goats	152:194	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	4	61	theme	TPSS	730:733	arg1	group					735:739	the TPSS group	726:739	the TPSS group	726:739	The results indicated that the TPSS group led to an elevation in the content of milk lactose relative to the CSSS.					
30415857	7	62	theme	higher	1135:1140	arg1	levels					1142:1147	higher levels	1135:1147	higher levels of peonidin (Peo) and malvidin-3-O-glucoside (M3G)	1135:1198	However, the TPSS resulted in higher levels of peonidin (Peo) and malvidin-3-O-glucoside (M3G) compared with the control.					
30415857	3	63	theme	Zea	665:667	arg1	L.					674:675	Zea mays L.	665:675	Zea mays L.	665:675	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	63	theme	Zea	665:667	arg1	corn					659:662	anthocyanin-rich purple corn	635:662	anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS)	635:696	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	64	theme	control	518:524	arg1	CSSS					527:530	the control (CSSS)	514:531	the control (CSSS)	514:531	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	64	theme	control	518:524	arg1	groups					502:507	The 2 study groups	490:507	The 2 study groups	490:507	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	4	65	theme	milk	779:782	arg1	lactose					784:790	milk lactose	779:790	milk lactose relative to the CSSS	779:811	The results indicated that the TPSS group led to an elevation in the content of milk lactose relative to the CSSS.					
30415857	1	66	dep	weight	270:275	arg1	deviation					311:319	mean ± standard deviation	295:319	mean ± standard deviation	295:319	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	3	67	theme	corn	659:662	arg1	PSS					693:695	PSS	693:695	PSS	693:695	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	67	theme	corn	659:662	arg1	silage					685:690	anthocyanin-rich purple corn (Zea mays L.) stover silage	635:690	anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS)	635:696	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	1	68	theme	mean	295:298	arg1	deviation					311:319	mean ± standard deviation	295:319	mean ± standard deviation	295:319	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	0	69	theme	anthocyanin	101:111	arg1	composition					113:123	anthocyanin composition	101:123	anthocyanin composition to the milk	101:135	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	8	70	theme	higher	1270:1275	arg1	level					1277:1281	a higher level	1268:1281	a higher level of superoxide dismutase (SOD)	1268:1311	Moreover, goats receiving TPSS exhibited a higher level of superoxide dismutase (SOD) in plasma and milk relative to the CSSS.					
30415857	3	71	theme	stover	678:683	arg1	PSS					693:695	PSS	693:695	PSS	693:695	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	71	theme	stover	678:683	arg1	silage					685:690	anthocyanin-rich purple corn (Zea mays L.) stover silage	635:690	anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS)	635:696	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	0	72	theme	abundant	66:73	arg1	anthocyanins					75:86	abundant anthocyanins	66:86	abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats	66:194	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	1	73	theme	standard	302:309	arg1	deviation					311:319	mean ± standard deviation	295:319	mean ± standard deviation	295:319	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	5	74	theme	anthocyanins	895:906	arg1	level					873:877	the level	869:877	the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk]	869:1017	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	3	75	theme	mays	669:672	arg1	L.					674:675	Zea mays L.	665:675	Zea mays L.	665:675	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	3	75	theme	mays	669:672	arg1	corn					659:662	anthocyanin-rich purple corn	635:662	anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS)	635:696	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	8	76	theme	dismutase	1297:1305	arg1	level					1277:1281	a higher level	1268:1281	a higher level of superoxide dismutase (SOD)	1268:1311	Moreover, goats receiving TPSS exhibited a higher level of superoxide dismutase (SOD) in plasma and milk relative to the CSSS.					
30415857	5	77	from	[i.e.	908:912	arg1	milk					1013:1016	milk	1013:1016	milk	1013:1016	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	1	78	theme	healthy	327:333	arg1	udders					351:356	healthy and symmetrical udders	327:356	healthy and symmetrical udders	327:356	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	10	79	dep	anthocyanins	1631:1642	arg1	Pel					1704:1706	Pel	1704:1706	Pel	1704:1706	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	79	dep	anthocyanins	1631:1642	arg1	anthocyanins					1631:1642	individual anthocyanins	1620:1642	individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel)	1620:1707	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	79	dep	anthocyanins	1631:1642	arg1	cyanidin-3-glucoside					1645:1664	cyanidin-3-glucoside	1645:1664	cyanidin-3-glucoside	1645:1664	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	79	dep	anthocyanins	1631:1642	arg1	M3G					1685:1687	M3G	1685:1687	M3G	1685:1687	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	79	dep	anthocyanins	1631:1642	arg1	cyanidin					1690:1697	cyanidin	1690:1697	cyanidin	1690:1697	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	79	dep	anthocyanins	1631:1642	arg1	delphinidin					1667:1677	delphinidin	1667:1677	delphinidin	1667:1677	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	10	79	dep	anthocyanins	1631:1642	arg1	Peo					1680:1682	Peo	1680:1682	Peo	1680:1682	In addition, the positive correlations were observed between individual anthocyanins (cyanidin-3-glucoside, delphinidin, Peo, M3G, cyanidin, and Pel) and total anthocyanins.					
30415857	11	80	dep	SOD	1898:1900	arg1	Pel					1893:1895	Pel	1893:1895	Pel	1893:1895	Specifically, stronger positive correlations were noted between several antioxidant enzymes and anthocyanin composition in milk (total antioxidant capacity and Pel; SOD and Peo as well as SOD and M3G).					
30415857	11	80	dep	SOD	1898:1900	arg1	capacity					1880:1887	total antioxidant capacity	1862:1887	total antioxidant capacity	1862:1887	Specifically, stronger positive correlations were noted between several antioxidant enzymes and anthocyanin composition in milk (total antioxidant capacity and Pel; SOD and Peo as well as SOD and M3G).					
30415857	0	81	theme	Purple	21:26	arg1	L.					43:44	Zea mays L.	34:44	Zea mays L.	34:44	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	0	81	theme	Purple	21:26	arg1	corn					28:31	Purple corn	21:31	Purple corn (Zea mays L.)	21:45	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	1	82	theme	body	265:268	arg1	weight					270:275	body weight	265:275	body weight	265:275	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	1	82	theme	body	265:268	arg1	goats					258:262	16 multiparous lactating Saanen dairy goats	220:262	16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders	220:356	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	11	83	theme	total	1862:1866	arg1	capacity					1880:1887	total antioxidant capacity	1862:1887	total antioxidant capacity	1862:1887	Specifically, stronger positive correlations were noted between several antioxidant enzymes and anthocyanin composition in milk (total antioxidant capacity and Pel; SOD and Peo as well as SOD and M3G).					
30415857	5	84	from	delphinidin	937:947	arg1	milk					1013:1016	milk	1013:1016	milk	1013:1016	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	9	85	theme	total	1536:1540	arg1	solids					1542:1547	total solids	1536:1547	total solids	1536:1547	Interestingly, some positive correlations were detected between the certain milk components [i.e., fat and total solids as well as fat and solids-not-fat (SNF); protein and SNF; and total solids and SNF].					
30415857	4	86	theme	relative	792:799	arg1	lactose					784:790	milk lactose	779:790	milk lactose relative to the CSSS	779:811	The results indicated that the TPSS group led to an elevation in the content of milk lactose relative to the CSSS.					
30415857	0	87	theme	mays	38:41	arg1	L.					43:44	Zea mays L.	34:44	Zea mays L.	34:44	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	0	87	theme	mays	38:41	arg1	corn					28:31	Purple corn	21:31	Purple corn (Zea mays L.)	21:45	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	5	88	theme	anthocyanin-rich	831:846	arg1	PSS					848:850	anthocyanin-rich PSS	831:850	anthocyanin-rich PSS	831:850	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	1	89	with	goats	258:262	arg1	udders					351:356	healthy and symmetrical udders	327:356	healthy and symmetrical udders	327:356	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	5	90	contain	had	852:854	arg1	inclusion					818:826	The inclusion	814:826	The inclusion of anthocyanin-rich PSS	814:850	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	5	90	contain	had	852:854	arg2	effect					859:864	no effect	856:864	no effect	856:864	The inclusion of anthocyanin-rich PSS had no effect on the level of 5 particular anthocyanins [i.e., cyanidin-3-glucoside, delphinidin, cyanidin, pelargonidin (Pel), as well as total anthocyanins in milk].					
30415857	8	91	theme	relative	1332:1339	arg1	plasma					1316:1321	plasma	1316:1321	plasma	1316:1321	Moreover, goats receiving TPSS exhibited a higher level of superoxide dismutase (SOD) in plasma and milk relative to the CSSS.					
30415857	3	92	theme	sticky	558:563	arg1	silage					577:582	sticky corn stover silage	558:582	sticky corn stover silage	558:582	The 2 study groups were the control (CSSS), in which goats were fed sticky corn stover silage, and the treatment (TPSS), in which goats were fed anthocyanin-rich purple corn (Zea mays L.) stover silage (PSS).					
30415857	0	93	dep	communication	6:18	arg1	stover					47:52	stover	47:52	stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats	47:194	Short communication: Purple corn (Zea mays L.) stover silage with abundant anthocyanins transferring anthocyanin composition to the milk and increasing antioxidant status of lactating dairy goats.					
30415857	12	94	theme	dairy	2072:2076	arg1	goats					2078:2082	lactating dairy goats	2062:2082	lactating dairy goats	2062:2082	Taken together, PSS with abundant anthocyanins can transfer anthocyanins to the milk and enhance the amount of antioxidants in lactating dairy goats.					
30415857	1	95	theme	multiparous	223:233	arg1	weight					270:275	body weight	265:275	body weight	265:275	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
30415857	1	95	theme	multiparous	223:233	arg1	goats					258:262	16 multiparous lactating Saanen dairy goats	220:262	16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders	220:356	The present study used 16 multiparous lactating Saanen dairy goats (body weight, 41.80 ± 2.92 kg; mean ± standard deviation) with healthy and symmetrical udders.					
31690358	9	0	theme	taxa	1719:1722	arg1	variations					1688:1697	Most variations	1683:1697	Most variations of faecal bacterial taxa under supplementation	1683:1744	Most variations of faecal bacterial taxa under supplementation were not observed in the hindgut.					
31690358	3	1	theme	×	501:501	arg1	design					518:523	a 2 × 2 Latin-square design	497:523	a 2 × 2 Latin-square design	497:523	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	2	2	theme	colonic	347:353	arg1	ecosystem					376:384	caecal, colonic and faecal microbial ecosystem	339:384	caecal, colonic and faecal microbial ecosystem	339:384	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	8	3	theme	volatile	1403:1410	arg1	acids					1418:1422	the volatile fatty acids	1399:1422	the volatile fatty acids	1399:1422	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	4	4	theme	end	820:822	arg1	products					824:831	microbial end products	810:831	microbial end products	810:831	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	3	5	theme	Latin-square	505:516	arg1	design					518:523	a 2 × 2 Latin-square design	497:523	a 2 × 2 Latin-square design	497:523	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	2	6	theme	microalgae	280:289	arg1	supplementation					320:334	a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation	241:334	supplementation	320:334	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	6	7	theme	microbial	943:951	arg1	ecosystem					953:961	the microbial ecosystem	939:961	the microbial ecosystem in the three digestive segments	939:993	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	7	8	theme	blood	1129:1133	arg1	parameters					1135:1144	the blood parameters	1125:1144	the blood parameters	1125:1144	However, no effect of the diet was observed on the blood parameters.					
31690358	4	9	theme	microbial	810:818	arg1	products					824:831	microbial end products	810:831	microbial end products	810:831	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	8	10	theme	Veillonellaceae	1608:1622	arg1	abundance					1595:1603	relative abundance	1586:1603	relative abundance of Veillonellaceae	1586:1622	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	8	11	theme	A	1255:1255	arg1	concentrations					1257:1270	serum amyloid A concentrations	1241:1270	serum amyloid A concentrations	1241:1270	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	4	12	theme	faecal	676:681	arg1	samples					683:689	Caecal, colonic and faecal samples	656:689	Caecal, colonic and faecal samples	656:689	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	4	13	theme	Caecal	656:661	arg1	samples					683:689	Caecal, colonic and faecal samples	656:689	Caecal, colonic and faecal samples	656:689	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	8	14	theme	relative	1482:1489	arg1	abundance					1491:1499	relative abundance	1482:1499	relative abundance of Family XIII Clostridiales	1482:1528	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	3	15	theme	fistulated	474:483	arg1	geldings					485:492	Six fistulated geldings	470:492	Six fistulated geldings in a 2 × 2 Latin-square design	470:523	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	4	16	theme	meal	728:731	arg1	times					739:743	the morning meal three times	716:743	the morning meal three times per diet	716:752	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	1	17	theme	hindgut	170:176	arg1	dysbiosis					178:186	hindgut dysbiosis	170:186	hindgut dysbiosis	170:186	Starchy diets can induce hindgut dysbiosis in horses.					
31690358	2	18	dep	yeast	243:247	arg1	cerevisiae					264:273	Saccharomyces cerevisiae	250:273	Saccharomyces cerevisiae	250:273	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	8	19	theme	Family	1504:1509	arg1	Clostridiales					1516:1528	Family XIII Clostridiales	1504:1528	Family XIII Clostridiales	1504:1528	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	6	20	theme	function	1031:1038	arg1	decrease					1046:1053	a decrease	1044:1053	a decrease of the fibrolytic one	1044:1075	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	6	20	theme	function	1031:1038	arg1	increase					1004:1011	an increase	1001:1011	an increase of the amylolytic function	1001:1038	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	3	21	from	geldings	485:492	arg1	design					518:523	a 2 × 2 Latin-square design	497:523	a 2 × 2 Latin-square design	497:523	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	4	22	theme	morning	720:726	arg1	times					739:743	the morning meal three times	716:743	the morning meal three times per diet	716:752	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	6	23	theme	amylolytic	1020:1029	arg1	function					1031:1038	the amylolytic function	1016:1038	the amylolytic function	1016:1038	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	2	24	theme	high-starch	451:461	arg1	diets					463:467	high-fibre or high-starch diets	437:467	high-fibre or high-starch diets	437:467	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	6	25	theme	starchy	909:915	arg1	diet					917:920	The starchy diet	905:920	The starchy diet	905:920	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	4	26	theme	bacterial	784:792	arg1	composition					794:804	bacterial composition	784:804	bacterial composition	784:804	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	5	27	theme	inflammatory	883:894	arg1	markers					896:902	inflammatory markers	883:902	inflammatory markers	883:902	Blood was simultaneously collected for measuring inflammatory markers.					
31690358	2	28	from	impact	231:236	arg1	parameters					412:421	blood inflammatory parameters	393:421	blood inflammatory parameters of horses fed high-fibre or high-starch diets	393:467	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	2	28	from	impact	231:236	arg1	ecosystem					376:384	caecal, colonic and faecal microbial ecosystem	339:384	caecal, colonic and faecal microbial ecosystem	339:384	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	6	29	theme	one	1073:1075	arg1	decrease					1046:1053	a decrease	1044:1053	a decrease of the fibrolytic one	1044:1075	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	6	29	theme	one	1073:1075	arg1	increase					1004:1011	an increase	1001:1011	an increase of the amylolytic function	1001:1038	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	0	30	theme	Dietary	0:6	arg1	composition					8:18	Dietary composition	0:18	Dietary composition	0:18	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	8	31	theme	Clostridiales	1516:1528	arg1	abundance					1491:1499	relative abundance	1482:1499	relative abundance of Family XIII Clostridiales	1482:1528	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	6	32	from	ecosystem	953:961	arg1	segments					986:993	the three digestive segments	966:993	the three digestive segments	966:993	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	2	33	dep	supplementation	320:334	arg1	limacinum					309:317	Aurantiochytrium limacinum	292:317	Aurantiochytrium limacinum	292:317	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	8	34	theme	significant	1181:1191	arg1	change					1193:1198	no significant change	1178:1198	no significant change	1178:1198	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	10	35	theme	fibrolytic	1850:1859	arg1	function					1861:1868	fibrolytic function	1850:1868	fibrolytic function whatever the diet	1850:1886	However, all variations suggested that supplementation could increase fibrolytic function whatever the diet and limit dysbiosis when the horses' diet changed from high fibre to high starch.					
31690358	0	36	theme	yeast/microalgae	24:39	arg1	supplementation					53:67	yeast/microalgae combination supplementation	24:67	yeast/microalgae combination supplementation	24:67	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	8	37	theme	XIII	1511:1514	arg1	Clostridiales					1516:1528	Family XIII Clostridiales	1504:1528	Family XIII Clostridiales	1504:1528	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	4	38	theme	5-d	758:760	arg1	intervals					762:770	5-d intervals	758:770	5-d intervals	758:770	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	8	39	theme	amyloid	1247:1253	arg1	concentrations					1257:1270	serum amyloid A concentrations	1241:1270	serum amyloid A concentrations	1241:1270	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	2	40	theme	horses	426:431	arg1	parameters					412:421	blood inflammatory parameters	393:421	blood inflammatory parameters of horses fed high-fibre or high-starch diets	393:467	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	9	41	located	observed	1755:1762	arg2	variations					1688:1697	Most variations	1683:1697	Most variations of faecal bacterial taxa under supplementation	1683:1744	Most variations of faecal bacterial taxa under supplementation were not observed in the hindgut.					
31690358	9	41	located	observed	1755:1762	arg1	hindgut					1771:1777	the hindgut	1767:1777	the hindgut	1767:1777	Most variations of faecal bacterial taxa under supplementation were not observed in the hindgut.					
31690358	8	42	theme	blood	1285:1289	arg1	count					1291:1295	complete blood count	1276:1295	complete blood count	1276:1295	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	3	43	theme	56/44	623:627	arg1	hay/barley					629:638	hay/barley	629:638	hay/barley	629:638	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	3	44	dep	period	582:587	arg1	hay					595:597	100 % hay	589:597	each period 100 % hay (4 weeks)	577:607	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	3	44	dep	period	582:587	arg1	weeks					602:606	4 weeks	600:606	4 weeks	600:606	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	1	45	theme	Starchy	145:151	arg1	diets					153:157	Starchy diets	145:157	Starchy diets	145:157	Starchy diets can induce hindgut dysbiosis in horses.					
31690358	4	46	theme	colonic	664:670	arg1	samples					683:689	Caecal, colonic and faecal samples	656:689	Caecal, colonic and faecal samples	656:689	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	9	47	theme	Most	1683:1686	arg1	variations					1688:1697	Most variations	1683:1697	Most variations of faecal bacterial taxa under supplementation	1683:1744	Most variations of faecal bacterial taxa under supplementation were not observed in the hindgut.					
31690358	3	48	theme	hay/barley	629:638	arg1	diet					640:643	a 56/44 hay/barley diet	621:643	a 56/44 hay/barley diet (3 weeks)	621:653	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	3	48	theme	hay/barley	629:638	arg1	weeks					648:652	3 weeks	646:652	3 weeks	646:652	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	8	49	theme	serum	1241:1245	arg1	concentrations					1257:1270	serum amyloid A concentrations	1241:1270	serum amyloid A concentrations	1241:1270	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	8	50	dep	cellulose-utilising	1308:1326	arg1	concentrations					1377:1390	concentrations	1377:1390	concentrations	1377:1390	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	2	51	theme	blood	393:397	arg1	parameters					412:421	blood inflammatory parameters	393:421	blood inflammatory parameters of horses fed high-fibre or high-starch diets	393:467	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	0	52	theme	combination	41:51	arg1	supplementation					53:67	yeast/microalgae combination supplementation	24:67	yeast/microalgae combination supplementation	24:67	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	0	53	from	ecosystem	92:100	arg1	faeces					127:132	faeces	127:132	faeces	127:132	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	0	53	from	ecosystem	92:100	arg1	colon					117:121	colon	117:121	colon	117:121	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	0	53	from	ecosystem	92:100	arg1	caecum					109:114	caecum	109:114	caecum	109:114	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	2	54	theme	high-fibre	437:446	arg1	diets					463:467	high-fibre or high-starch diets	437:467	high-fibre or high-starch diets	437:467	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	2	55	theme	inflammatory	399:410	arg1	parameters					412:421	blood inflammatory parameters	393:421	blood inflammatory parameters of horses fed high-fibre or high-starch diets	393:467	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	8	56	theme	fatty	1412:1416	arg1	acids					1418:1422	the volatile fatty acids	1399:1422	the volatile fatty acids	1399:1422	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	8	57	theme	lactate-utilising	1350:1366	arg1	bacteria					1368:1375	lactate-utilising bacteria	1350:1375	lactate-utilising bacteria	1350:1375	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	4	58	dep	h	708:708	arg1	times					739:743	the morning meal three times	716:743	the morning meal three times per diet	716:752	Caecal, colonic and faecal samples were collected 4 h after the morning meal three times per diet, at 5-d intervals, to measure bacterial composition and microbial end products.					
31690358	0	59	theme	horses	137:142	arg1	faeces					127:132	faeces	127:132	faeces	127:132	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	0	59	theme	horses	137:142	arg1	colon					117:121	colon	117:121	colon	117:121	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	0	59	theme	horses	137:142	arg1	caecum					109:114	caecum	109:114	caecum	109:114	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	8	60	theme	irrespective	1561:1572	arg1	caecum					1543:1548	caecum	1543:1548	caecum	1543:1548	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	9	61	theme	faecal	1702:1707	arg1	taxa					1719:1722	faecal bacterial taxa	1702:1722	faecal bacterial taxa under supplementation	1702:1744	Most variations of faecal bacterial taxa under supplementation were not observed in the hindgut.					
31690358	8	62	theme	lactate	1428:1434	arg1	concentrations					1436:1449	lactate concentrations	1428:1449	lactate concentrations	1428:1449	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	3	63	theme	%	593:593	arg1	hay					595:597	100 % hay	589:597	each period 100 % hay (4 weeks)	577:607	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	3	63	theme	%	593:593	arg1	weeks					602:606	4 weeks	600:606	4 weeks	600:606	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	2	64	theme	caecal	339:344	arg1	ecosystem					376:384	caecal, colonic and faecal microbial ecosystem	339:384	caecal, colonic and faecal microbial ecosystem	339:384	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	10	65	theme	high	1957:1960	arg1	starch					1962:1967	high starch	1957:1967	high starch	1957:1967	However, all variations suggested that supplementation could increase fibrolytic function whatever the diet and limit dysbiosis when the horses' diet changed from high fibre to high starch.					
31690358	9	66	theme	bacterial	1709:1717	arg1	taxa					1719:1722	faecal bacterial taxa	1702:1722	faecal bacterial taxa under supplementation	1702:1744	Most variations of faecal bacterial taxa under supplementation were not observed in the hindgut.					
31690358	2	67	theme	present	203:209	arg1	study					211:215	The present study	199:215	The present study	199:215	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	7	68	located	observed	1113:1120	arg2	effect					1090:1095	no effect	1087:1095	no effect of the diet	1087:1107	However, no effect of the diet was observed on the blood parameters.					
31690358	7	68	located	observed	1113:1120	arg1	parameters					1135:1144	the blood parameters	1125:1144	the blood parameters	1125:1144	However, no effect of the diet was observed on the blood parameters.					
31690358	6	69	theme	fibrolytic	1062:1071	arg1	one					1073:1075	the fibrolytic one	1058:1075	the fibrolytic one	1058:1075	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	8	70	located	found	1204:1208	arg2	change					1193:1198	no significant change	1178:1198	no significant change	1178:1198	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	8	70	located	found	1204:1208	arg1	count					1291:1295	complete blood count	1276:1295	complete blood count	1276:1295	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	8	70	located	found	1204:1208	arg1	lipopolysaccharides					1213:1231	lipopolysaccharides	1213:1231	lipopolysaccharides	1213:1231	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	8	70	located	found	1204:1208	arg1	cellulose-utilising					1308:1326	cellulose-utilising	1308:1326	cellulose-utilising	1308:1326	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	8	70	located	found	1204:1208	arg1	concentrations					1257:1270	serum amyloid A concentrations	1241:1270	serum amyloid A concentrations	1241:1270	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	8	70	located	found	1204:1208	arg1	PG-E2					1234:1238	PG-E2	1234:1238	PG-E2	1234:1238	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	8	71	theme	complete	1276:1283	arg1	count					1291:1295	complete blood count	1276:1295	complete blood count	1276:1295	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	7	72	theme	diet	1104:1107	arg1	effect					1090:1095	no effect	1087:1095	no effect of the diet	1087:1107	However, no effect of the diet was observed on the blood parameters.					
31690358	2	73	theme	supplementation	320:334	arg1	impact					231:236	the impact	227:236	the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets	227:467	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	8	74	theme	hay/barley	1646:1655	arg1	diet					1657:1660	the hay/barley diet	1642:1660	the hay/barley diet	1642:1660	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	6	75	theme	digestive	976:984	arg1	segments					986:993	the three digestive segments	966:993	the three digestive segments	966:993	The starchy diet clearly modified the microbial ecosystem in the three digestive segments, with an increase of the amylolytic function and a decrease of the fibrolytic one.					
31690358	2	76	theme	yeast	243:247	arg1	impact					231:236	the impact	227:236	the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets	227:467	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	3	77	dep	received	561:568	arg1	followed					609:616	followed	609:616	followed by a 56/44 hay/barley diet (3 weeks)	609:653	Six fistulated geldings in a 2 × 2 Latin-square design were alternatively supplemented and received during each period 100 % hay (4 weeks) followed by a 56/44 hay/barley diet (3 weeks).					
31690358	2	78	theme	microbial	366:374	arg1	ecosystem					376:384	caecal, colonic and faecal microbial ecosystem	339:384	caecal, colonic and faecal microbial ecosystem	339:384	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	8	79	theme	relative	1586:1593	arg1	abundance					1595:1603	relative abundance	1586:1603	relative abundance of Veillonellaceae	1586:1622	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	0	80	theme	microbial	82:90	arg1	ecosystem					92:100	the microbial ecosystem	78:100	the microbial ecosystem in the caecum, colon and faeces of horses	78:142	Dietary composition and yeast/microalgae combination supplementation modulate the microbial ecosystem in the caecum, colon and faeces of horses.					
31690358	8	81	theme	diet	1577:1580	arg1	irrespective					1561:1572	irrespective	1561:1572	irrespective	1561:1572	When horses were supplemented, no significant change was found in lipopolysaccharides, PG-E2, serum amyloid A concentrations and complete blood count neither in cellulose-utilising, starch-utilising and lactate-utilising bacteria concentrations nor in the volatile fatty acids and lactate concentrations and pH. Under supplementation, relative abundance of Family XIII Clostridiales increased in caecum and faeces irrespective of diet and relative abundance of Veillonellaceae was higher during the hay/barley diet in colon and faeces.					
31690358	2	82	theme	faecal	359:364	arg1	ecosystem					376:384	caecal, colonic and faecal microbial ecosystem	339:384	caecal, colonic and faecal microbial ecosystem	339:384	The present study evaluated the impact of a yeast (Saccharomyces cerevisiae) and microalgae (Aurantiochytrium limacinum) supplementation on caecal, colonic and faecal microbial ecosystem and on blood inflammatory parameters of horses fed high-fibre or high-starch diets.					
31690358	10	83	dep	diet	1883:1886	arg1	the					1879:1881	the	1879:1881	the	1879:1881	However, all variations suggested that supplementation could increase fibrolytic function whatever the diet and limit dysbiosis when the horses' diet changed from high fibre to high starch.					
30124869	16	0	theme	moderate	2279:2286	arg1	precision					2288:2296	moderate precision	2279:2296	moderate precision	2279:2296	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	3	1	theme	dry	432:434	arg1	DMI					451:453	DMI	451:453	DMI	451:453	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	1	theme	dry	432:434	arg1	intake					443:448	dry matter intake	432:448	dry matter intake (DMI)	432:454	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	16	2	dep	D.	2158:2159	arg1	predicted					2259:2267	predicted	2259:2267	could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69)	2250:2331	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	16	2	dep	D.	2158:2159	arg1	unaffected					2220:2229	unaffected	2220:2229	were unaffected by breed type	2215:2243	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	3	3	theme	matter	436:441	arg1	DMI					451:453	DMI	451:453	DMI	451:453	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	3	theme	matter	436:441	arg1	intake					443:448	dry matter intake	432:448	dry matter intake (DMI)	432:454	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	16	4	theme	=	2317:2317	arg1	uCCC					2312:2315	uCCC	2312:2315	uCCC = 0.63 to 0.69	2312:2330	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	9	5	used	used	1314:1317	arg2	regression					1256:1265	Linear mixed-effects regression	1235:1265	Linear mixed-effects regression	1235:1265	Linear mixed-effects regression, weighted by 1/standard error of the mean, was used to derive models using data from 51 peer-reviewed publications.					
30124869	1	6	theme	rumen	157:161	arg1	microbiome					163:172	the rumen microbiome	153:172	the rumen microbiome	153:172	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	1	6	theme	rumen	157:161	arg1	predictor					199:207	an accurate predictor	187:207	an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition	187:299	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	4	7	theme	detergent	673:681	arg1	fiber					683:687	acid detergent fiber	668:687	acid detergent fiber	668:687	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	10	8	theme	root-estimated	1522:1535	arg1	variance					1537:1544	root-estimated variance	1522:1544	root-estimated variance associated with study and error	1522:1576	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	16	9	theme	diversity	2194:2202	arg1	indicators					2204:2213	species richness and diversity indicators	2173:2213	species richness and diversity indicators	2173:2213	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	10	10	theme	correlation	1463:1473	arg1	coefficient					1475:1485	concordance correlation coefficient	1451:1485	concordance correlation coefficient	1451:1485	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	11	11	theme	MY	1656:1657	arg1	model					1659:1663	The MY model	1652:1663	The MY model using D + M + E predictors	1652:1690	The MY model using D + M + E predictors outperformed all other MY models (uCCC = 0.71).					
30124869	1	12	theme	accurate	190:197	arg1	microbiome					163:172	the rumen microbiome	153:172	the rumen microbiome	153:172	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	1	12	theme	accurate	190:197	arg1	predictor					199:207	an accurate predictor	187:207	an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition	187:299	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	4	13	theme	ether	705:709	arg1	extract					711:717	ether extract	705:717	ether extract	705:717	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	4	13	theme	ether	705:709	arg1	protein					696:702	crude protein	690:702	crude protein	690:702	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	18	14	theme	independent	2516:2526	arg1	influences					2528:2537	these independent influences	2510:2537	these independent influences	2510:2537	Further research is necessary to determine the reasons for these independent influences.					
30124869	6	15	theme	rumen	995:999	arg1	fraction					1010:1017	rumen sampling fraction	995:1017	rumen sampling fraction (fluid or solid)	995:1034	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	3	16	theme	different	503:511	arg1	sets					513:516	different sets	503:516	different sets of independent variables	503:541	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	16	theme	different	503:511	arg1	variables					533:541	independent variables	521:541	independent variables	521:541	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	15	17	theme	Beef	2005:2008	arg1	FE					2010:2011	Beef FE	2005:2011	Beef FE	2005:2011	Beef FE could only be modeled using D variables.					
30124869	1	18	theme	meta-analysis	114:126	arg1	aim					102:104	The central aim	90:104	The central aim of this meta-analysis	90:126	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	16	19	theme	phyla	2147:2151	arg1	abundances					2112:2121	relative abundances	2103:2121	relative abundances of most rumen bacterial phyla	2103:2151	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	11	20	theme	M	1675:1675	arg1	predictors					1681:1690	D + M + E predictors	1671:1690	D + M + E predictors	1671:1690	The MY model using D + M + E predictors outperformed all other MY models (uCCC = 0.71).					
30124869	10	21	dep	removed	1506:1512	arg1	effects					1498:1504	study effects	1492:1504	study effects removed (uCCC)	1492:1519	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	10	21	dep	removed	1506:1512	arg1	uCCC					1515:1518	uCCC	1515:1518	uCCC	1515:1518	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	5	22	theme	relative	805:812	arg1	abundance					814:822	relative abundance	805:822	relative abundance of 3 major rumen bacterial phyla	805:855	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	15	23	theme	D	2041:2041	arg1	variables					2043:2051	D variables	2041:2051	D variables	2041:2051	Beef FE could only be modeled using D variables.					
30124869	11	24	theme	MY	1715:1716	arg1	models					1718:1723	all other MY models	1705:1723	all other MY models	1705:1723	The MY model using D + M + E predictors outperformed all other MY models (uCCC = 0.71).					
30124869	9	25	theme	1/standard	1280:1289	arg1	error					1291:1295	1/standard error	1280:1295	1/standard error of the mean	1280:1307	Linear mixed-effects regression, weighted by 1/standard error of the mean, was used to derive models using data from 51 peer-reviewed publications.					
30124869	0	26	from	Variation	0:8	arg1	performance					20:30	animal performance	13:30	animal performance	13:30	Variation in animal performance explained by the rumen microbiome or by diet composition.					
30124869	4	27	theme	Diet	591:594	arg1	variables					596:604	Diet variables	591:604	Diet variables	591:604	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	9	28	theme	mean	1304:1307	arg1	error					1291:1295	1/standard error	1280:1295	1/standard error of the mean	1280:1307	Linear mixed-effects regression, weighted by 1/standard error of the mean, was used to derive models using data from 51 peer-reviewed publications.					
30124869	16	29	theme	richness	2181:2188	arg1	indicators					2204:2213	species richness and diversity indicators	2173:2213	species richness and diversity indicators	2173:2213	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	10	30	theme	response	1403:1410	arg1	variable					1412:1419	the same response variable	1394:1419	the same response variable	1394:1419	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	18	31	theme	Further	2451:2457	arg1	research					2459:2466	Further research	2451:2466	Further research	2451:2466	Further research is necessary to determine the reasons for these independent influences.					
30124869	7	32	used	used	1060:1063	arg2	models					1053:1058	models	1053:1058	models	1053:1058	A second set of models used D and E variables as predictors for the microbiome.					
30124869	7	32	used	used	1060:1063	arg2	set					1046:1048	A second set	1037:1048	A second set of models	1037:1058	A second set of models used D and E variables as predictors for the microbiome.					
30124869	3	33	theme	milk	389:392	arg1	MY					401:402	MY	401:402	MY	401:402	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	33	theme	milk	389:392	arg1	yield					394:398	milk yield	389:398	milk yield (MY)	389:403	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	10	34	theme	criterion	1612:1620	arg1	values					1622:1627	corrected Akaike information criterion values	1583:1627	corrected Akaike information criterion values	1583:1627	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	3	35	theme	beef	489:492	arg1	FE					494:495	beef FE	489:495	beef FE	489:495	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	36	theme	average	406:412	arg1	ADG					426:428	ADG	426:428	ADG	426:428	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	36	theme	average	406:412	arg1	gain					420:423	average daily gain	406:423	average daily gain (ADG)	406:429	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	0	37	theme	animal	13:18	arg1	performance					20:30	animal performance	13:30	animal performance	13:30	Variation in animal performance explained by the rumen microbiome or by diet composition.					
30124869	1	38	theme	central	94:100	arg1	aim					102:104	The central aim	90:104	The central aim of this meta-analysis	90:126	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	6	39	dep	fraction	1010:1017	arg1	solid					1029:1033	solid	1029:1033	solid	1029:1033	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	6	39	dep	fraction	1010:1017	arg1	fluid					1020:1024	fluid	1020:1024	fluid	1020:1024	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	3	40	theme	variables	533:541	arg1	diet					544:547	diet	544:547	diet (D)	544:551	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	40	theme	variables	533:541	arg1	sets					513:516	different sets	503:516	different sets of independent variables	503:541	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	40	theme	variables	533:541	arg1	variables					533:541	independent variables	521:541	independent variables	521:541	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	40	theme	variables	533:541	arg1	D					550:550	D	550:550	D	550:550	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	8	41	theme	microbiome	1145:1154	arg1	sets					1162:1165	microbiome model sets	1145:1165	microbiome model sets	1145:1165	For both the production and microbiome model sets, predictor variable sets were used individually and in combination.					
30124869	11	42	theme	D	1671:1671	arg1	predictors					1681:1690	D + M + E predictors	1671:1690	D + M + E predictors	1671:1690	The MY model using D + M + E predictors outperformed all other MY models (uCCC = 0.71).					
30124869	16	43	theme	abundances	2112:2121	arg1	predictor					2090:2098	a better predictor	2081:2098	a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69)	2081:2331	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	5	44	theme	rumen	835:839	arg1	phyla					851:855	3 major rumen bacterial phyla	827:855	3 major rumen bacterial phyla	827:855	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	10	45	theme	Akaike	1593:1598	arg1	criterion					1612:1620	corrected Akaike information criterion	1583:1620	corrected Akaike information criterion values	1583:1627	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	4	46	theme	detergent	651:659	arg1	fiber					661:665	neutral detergent fiber	643:665	neutral detergent fiber	643:665	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	10	47	theme	concordance	1451:1461	arg1	coefficient					1475:1485	concordance correlation coefficient	1451:1485	concordance correlation coefficient	1451:1485	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	13	48	theme	D	1880:1880	arg1	variables					1882:1890	D variables	1880:1890	D variables	1880:1890	Interestingly, M + E was more successful at predicting DMI than any model using D variables.					
30124869	5	49	theme	phyla	851:855	arg1	richness					866:873	species richness	858:873	species richness	858:873	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	5	49	theme	phyla	851:855	arg1	diversity					888:896	species diversity	880:896	species diversity	880:896	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	5	49	theme	phyla	851:855	arg1	abundance					814:822	relative abundance	805:822	relative abundance of 3 major rumen bacterial phyla	805:855	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	6	50	theme	breed	949:953	arg1	type					955:958	breed type	949:958	breed type (dairy, beef, or Bos indicus)	949:988	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	8	51	theme	predictor	1168:1176	arg1	sets					1187:1190	predictor variable sets	1168:1190	predictor variable sets	1168:1190	For both the production and microbiome model sets, predictor variable sets were used individually and in combination.					
30124869	16	52	theme	bacterial	2137:2145	arg1	phyla					2147:2151	most rumen bacterial phyla	2126:2151	most rumen bacterial phyla	2126:2151	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	7	53	theme	second	1039:1044	arg1	models					1053:1058	models	1053:1058	models	1053:1058	A second set of models used D and E variables as predictors for the microbiome.					
30124869	7	53	theme	second	1039:1044	arg1	set					1046:1048	A second set	1037:1048	A second set of models	1037:1058	A second set of models used D and E variables as predictors for the microbiome.					
30124869	14	54	theme	dairy	1904:1908	arg1	FE					1910:1911	dairy FE	1904:1911	dairy FE	1904:1911	Similarly, dairy FE was more accurately predicted by M + E than D, albeit only slightly (uCCC = 0.69 vs. 0.65).					
30124869	6	55	dep	type	955:958	arg1	beef					968:971	beef	968:971	beef	968:971	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	6	55	dep	type	955:958	arg1	dairy					961:965	dairy	961:965	dairy	961:965	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	6	55	dep	type	955:958	arg1	indicus					981:987	indicus	981:987	indicus	981:987	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	10	56	theme	study	1492:1496	arg1	effects					1498:1504	study effects	1492:1504	study effects removed (uCCC)	1492:1519	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	10	56	theme	study	1492:1496	arg1	uCCC					1515:1518	uCCC	1515:1518	uCCC	1515:1518	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	1	57	theme	performance	212:222	arg1	microbiome					163:172	the rumen microbiome	153:172	the rumen microbiome	153:172	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	1	57	theme	performance	212:222	arg1	predictor					199:207	an accurate predictor	187:207	an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition	187:299	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	16	58	dep	0.69	2327:2330	arg1	to					2324:2325	to	2324:2325	to	2324:2325	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	10	59	theme	coefficient	1475:1485	arg1	basis					1442:1446	the basis	1438:1446	the basis of concordance correlation coefficient	1438:1485	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	1	60	theme	beef	227:230	arg1	cattle					242:247	beef and dairy cattle	227:247	beef and dairy cattle compared with predictions based on diet composition	227:299	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	16	61	theme	breed	2063:2067	arg1	type					2069:2072	breed type	2063:2072	breed type	2063:2072	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	6	62	theme	Experimental	899:910	arg1	variables					912:920	Experimental variables	899:920	Experimental variables	899:920	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	1	63	theme	dairy	236:240	arg1	cattle					242:247	beef and dairy cattle	227:247	beef and dairy cattle compared with predictions based on diet composition	227:299	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	4	64	theme	crude	690:694	arg1	extract					711:717	ether extract	705:717	ether extract	705:717	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	4	64	theme	crude	690:694	arg1	carbohydrate					729:740	nonfiber carbohydrate	720:740	nonfiber carbohydrate	720:740	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	4	64	theme	crude	690:694	arg1	protein					696:702	crude protein	690:702	crude protein	690:702	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	17	65	theme	various	2419:2425	arg1	aspects					2427:2433	various aspects	2419:2433	various aspects of performance	2419:2448	This analysis suggests that diet and the microbiome may exert independent effects on various aspects of performance.					
30124869	16	66	theme	better	2083:2088	arg1	predictor					2090:2098	a better predictor	2081:2098	a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69)	2081:2331	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	16	67	dep	=	2317:2317	arg1	0.69					2327:2330	0.69	2327:2330	0.69	2327:2330	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	4	68	theme	acid	668:671	arg1	fiber					683:687	acid detergent fiber	668:687	acid detergent fiber	668:687	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	4	69	dep	percentages	762:772	arg1	forage					755:760	forage	755:760	forage	755:760	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	4	70	theme	neutral	643:649	arg1	fiber					661:665	neutral detergent fiber	643:665	neutral detergent fiber	643:665	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	3	71	theme	dairy	457:461	arg1	FE					480:481	FE	480:481	FE	480:481	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	71	theme	dairy	457:461	arg1	efficiency					468:477	dairy feed efficiency	457:477	dairy feed efficiency (FE)	457:482	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	4	72	theme	nonfiber	720:727	arg1	carbohydrate					729:740	nonfiber carbohydrate	720:740	nonfiber carbohydrate	720:740	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	4	72	theme	nonfiber	720:727	arg1	protein					696:702	crude protein	690:702	crude protein	690:702	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	8	73	used	used	1197:1200	arg2	sets					1187:1190	predictor variable sets	1168:1190	predictor variable sets	1168:1190	For both the production and microbiome model sets, predictor variable sets were used individually and in combination.					
30124869	5	74	theme	species	880:886	arg1	diversity					888:896	species diversity	880:896	species diversity	880:896	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	16	75	theme	breed	2234:2238	arg1	type					2240:2243	breed type	2234:2243	breed type	2234:2243	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	11	76	theme	E	1679:1679	arg1	predictors					1681:1690	D + M + E predictors	1671:1690	D + M + E predictors	1671:1690	The MY model using D + M + E predictors outperformed all other MY models (uCCC = 0.71).					
30124869	9	77	theme	mixed-effects	1242:1254	arg1	regression					1256:1265	Linear mixed-effects regression	1235:1265	Linear mixed-effects regression	1235:1265	Linear mixed-effects regression, weighted by 1/standard error of the mean, was used to derive models using data from 51 peer-reviewed publications.					
30124869	6	78	theme	sampling	1001:1008	arg1	fraction					1010:1017	rumen sampling fraction	995:1017	rumen sampling fraction (fluid or solid)	995:1034	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	1	79	from	predictor	199:207	arg1	cattle					242:247	beef and dairy cattle	227:247	beef and dairy cattle compared with predictions based on diet composition	227:299	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	5	80	theme	species	858:864	arg1	richness					866:873	species richness	858:873	species richness	858:873	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	10	81	theme	corrected	1583:1591	arg1	criterion					1612:1620	corrected Akaike information criterion	1583:1620	corrected Akaike information criterion values	1583:1627	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	11	82	theme	other	1709:1713	arg1	models					1718:1723	all other MY models	1705:1723	all other MY models	1705:1723	The MY model using D + M + E predictors outperformed all other MY models (uCCC = 0.71).					
30124869	0	83	theme	diet	72:75	arg1	composition					77:87	diet composition	72:87	diet composition	72:87	Variation in animal performance explained by the rumen microbiome or by diet composition.					
30124869	16	84	dep	precision	2288:2296	arg1	uCCC					2312:2315	uCCC	2312:2315	uCCC = 0.63 to 0.69	2312:2330	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	16	85	theme	species	2173:2179	arg1	richness					2181:2188	species richness	2173:2188	species richness	2173:2188	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	3	86	theme	feed	463:466	arg1	FE					480:481	FE	480:481	FE	480:481	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	86	theme	feed	463:466	arg1	efficiency					468:477	dairy feed efficiency	457:477	dairy feed efficiency (FE)	457:482	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	17	87	theme	independent	2396:2406	arg1	effects					2408:2414	independent effects	2396:2414	independent effects	2396:2414	This analysis suggests that diet and the microbiome may exert independent effects on various aspects of performance.					
30124869	5	88	theme	Microbiome	775:784	arg1	variables					786:794	Microbiome variables	775:794	Microbiome variables	775:794	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	10	89	theme	same	1398:1401	arg1	variable					1412:1419	the same response variable	1394:1419	the same response variable	1394:1419	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	9	90	from	publications	1369:1380	arg1	data					1342:1345	data	1342:1345	data from 51 peer-reviewed publications	1342:1380	Linear mixed-effects regression, weighted by 1/standard error of the mean, was used to derive models using data from 51 peer-reviewed publications.					
30124869	7	91	theme	E	1071:1071	arg1	variables					1073:1081	D and E variables	1065:1081	D and E variables	1065:1081	A second set of models used D and E variables as predictors for the microbiome.					
30124869	7	91	theme	E	1071:1071	arg1	predictors					1086:1095	predictors	1086:1095	predictors for the microbiome	1086:1114	A second set of models used D and E variables as predictors for the microbiome.					
30124869	2	92	theme	models	339:344	arg1	models					339:344	models	339:344	models	339:344	To support this comparison, a set of models was derived and compared.					
30124869	2	92	theme	models	339:344	arg1	set					332:334	a set	330:334	a set of models	330:344	To support this comparison, a set of models was derived and compared.					
30124869	4	93	theme	organic	627:633	arg1	matter					635:640	organic matter	627:640	organic matter	627:640	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	7	94	theme	D	1065:1065	arg1	variables					1073:1081	D and E variables	1065:1081	D and E variables	1065:1081	A second set of models used D and E variables as predictors for the microbiome.					
30124869	7	94	theme	D	1065:1065	arg1	predictors					1086:1095	predictors	1086:1095	predictors for the microbiome	1086:1114	A second set of models used D and E variables as predictors for the microbiome.					
30124869	3	95	theme	independent	521:531	arg1	variables					533:541	independent variables	521:541	independent variables	521:541	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	96	theme	daily	414:418	arg1	ADG					426:428	ADG	426:428	ADG	426:428	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	3	96	theme	daily	414:418	arg1	gain					420:423	average daily gain	406:423	average daily gain (ADG)	406:429	Models predicted milk yield (MY), average daily gain (ADG), dry matter intake (DMI), dairy feed efficiency (FE), and beef FE using different sets of independent variables: diet (D), microbial (M), and experimental (E).					
30124869	5	97	theme	major	829:833	arg1	phyla					851:855	3 major rumen bacterial phyla	827:855	3 major rumen bacterial phyla	827:855	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	7	98	theme	models	1053:1058	arg1	models					1053:1058	models	1053:1058	models	1053:1058	A second set of models used D and E variables as predictors for the microbiome.					
30124869	7	98	theme	models	1053:1058	arg1	set					1046:1048	A second set	1037:1048	A second set of models	1037:1058	A second set of models used D and E variables as predictors for the microbiome.					
30124869	10	99	theme	information	1600:1610	arg1	criterion					1612:1620	corrected Akaike information criterion	1583:1620	corrected Akaike information criterion values	1583:1627	Models for the same response variable were compared on the basis of concordance correlation coefficient with study effects removed (uCCC), root-estimated variance associated with study and error, and corrected Akaike information criterion values, wherever appropriate.					
30124869	0	100	theme	rumen	49:53	arg1	microbiome					55:64	the rumen microbiome	45:64	the rumen microbiome	45:64	Variation in animal performance explained by the rumen microbiome or by diet composition.					
30124869	1	101	theme	diet	284:287	arg1	composition					289:299	diet composition	284:299	diet composition	284:299	The central aim of this meta-analysis was to determine whether the rumen microbiome can serve as an accurate predictor of performance in beef and dairy cattle compared with predictions based on diet composition.					
30124869	16	102	theme	relative	2103:2110	arg1	abundances					2112:2121	relative abundances	2103:2121	relative abundances of most rumen bacterial phyla	2103:2151	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	5	103	theme	bacterial	841:849	arg1	phyla					851:855	3 major rumen bacterial phyla	827:855	3 major rumen bacterial phyla	827:855	Microbiome variables included relative abundance of 3 major rumen bacterial phyla, species richness, and species diversity.					
30124869	8	104	theme	model	1156:1160	arg1	sets					1162:1165	microbiome model sets	1145:1165	microbiome model sets	1145:1165	For both the production and microbiome model sets, predictor variable sets were used individually and in combination.					
30124869	9	105	theme	peer-reviewed	1355:1367	arg1	publications					1369:1380	51 peer-reviewed publications	1352:1380	51 peer-reviewed publications	1352:1380	Linear mixed-effects regression, weighted by 1/standard error of the mean, was used to derive models using data from 51 peer-reviewed publications.					
30124869	16	106	with	D	2272:2272	arg1	accuracy					2302:2309	accuracy	2302:2309	accuracy	2302:2309	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	16	106	with	D	2272:2272	arg1	precision					2288:2296	moderate precision	2279:2296	moderate precision	2279:2296	Overall, breed type proved a better predictor of relative abundances of most rumen bacterial phyla than D. Conversely, species richness and diversity indicators were unaffected by breed type, but could be predicted by D with moderate precision and accuracy (uCCC = 0.63 to 0.69).					
30124869	4	107	theme	dry	615:617	arg1	matter					619:624	dry matter	615:624	dry matter	615:624	Diet variables included dry matter, organic matter, neutral detergent fiber, acid detergent fiber, crude protein, ether extract, nonfiber carbohydrate, starch, and forage percentages.					
30124869	17	108	theme	performance	2438:2448	arg1	aspects					2427:2433	various aspects	2419:2433	various aspects of performance	2419:2448	This analysis suggests that diet and the microbiome may exert independent effects on various aspects of performance.					
30124869	9	109	theme	Linear	1235:1240	arg1	regression					1256:1265	Linear mixed-effects regression	1235:1265	Linear mixed-effects regression	1235:1265	Linear mixed-effects regression, weighted by 1/standard error of the mean, was used to derive models using data from 51 peer-reviewed publications.					
30124869	6	110	theme	publication	931:941	arg1	year					943:946	publication year	931:946	publication year	931:946	Experimental variables included publication year, breed type (dairy, beef, or Bos indicus), and rumen sampling fraction (fluid or solid).					
30124869	8	111	theme	variable	1178:1185	arg1	sets					1187:1190	predictor variable sets	1168:1190	predictor variable sets	1168:1190	For both the production and microbiome model sets, predictor variable sets were used individually and in combination.					
30989865	5	0	theme	tumor	628:632	arg1	factor					643:648	tumor necrosis factor	628:648	tumor necrosis factor(TNF-α)	628:655	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	5	0	theme	tumor	628:632	arg1	TNF-α					650:654	TNF-α	650:654	TNF-α	650:654	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	10	1	theme	NO	1336:1337	arg1	levels					1326:1331	the release levels	1314:1331	the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1314:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	2	theme	AKT	1436:1438	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	2	theme	AKT	1436:1438	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	2	theme	AKT	1436:1438	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	2	theme	AKT	1436:1438	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	2	theme	AKT	1436:1438	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	7	3	theme	Reverse	801:807	arg1	RT-PCR					849:854	RT-PCR	849:854	RT-PCR	849:854	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	7	3	theme	Reverse	801:807	arg1	reaction					840:847	Reverse transcription-polymerase chain reaction	801:847	Reverse transcription-polymerase chain reaction(RT-PCR) method	801:862	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	9	4	theme	chemical	1215:1222	arg1	constituents					1224:1235	the possible chemical constituents	1202:1235	the possible chemical constituents in fraction A	1202:1249	In addition,LC-MS and database were used to identify the possible chemical constituents in fraction A.					
30989865	10	5	theme	TNF-α	1354:1358	arg1	levels					1326:1331	the release levels	1314:1331	the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1314:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	6	theme	Rela	1456:1459	arg1	mRNA					1461:1464	Rela mRNA	1456:1464	Rela mRNA	1456:1464	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	7	from	expression	1387:1396	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	7	from	expression	1387:1396	arg1	supernatant					1367:1377	the supernatant	1363:1377	the supernatant	1363:1377	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	8	from	K	1434:1434	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	9	theme	IL-1β	1344:1348	arg1	levels					1326:1331	the release levels	1314:1331	the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1314:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	10	theme	cells	136:140	arg1	carrier					175:181	a carrier	173:181	a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway	173:434	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	2	10	theme	cells	136:140	arg1	model					155:159	7 cells inflammation model	134:159	7 cells inflammation model	134:159	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	7	11	theme	Rela	959:962	arg1	mRNA					964:967	Rela mRNA	959:967	Rela mRNA	959:967	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	13	12	theme	protein	1605:1611	arg1	expressions					1622:1632	protein and mRNA expressions	1605:1632	expressions	1622:1632	01) and significantly inhibit the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions(P<0.					
30989865	10	13	theme	NF-κB1	1445:1450	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	13	theme	NF-κB1	1445:1450	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	13	theme	NF-κB1	1445:1450	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	13	theme	NF-κB1	1445:1450	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	13	theme	NF-κB1	1445:1450	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	8	14	theme	phosphorylated	1030:1043	arg1	protein					1065:1071	phosphorylated and total PI3 K/AKT protein	1030:1071	phosphorylated and total PI3 K/AKT protein	1030:1071	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	10	15	from	IL-6	1401:1404	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	16	from	AKT	1436:1438	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	5	17	theme	necrosis	634:641	arg1	factor					643:648	tumor necrosis factor	628:648	tumor necrosis factor(TNF-α)	628:655	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	5	17	theme	necrosis	634:641	arg1	TNF-α					650:654	TNF-α	650:654	TNF-α	650:654	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	5	18	theme	interleukin-1β	602:615	arg1	A					564:564	fraction A	555:564	fraction A	555:564	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	5	18	theme	interleukin-1β	602:615	arg1	contents					570:577	the contents	566:577	the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264	566:684	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	13	19	theme	mRNA	1617:1620	arg1	expressions					1622:1632	protein and mRNA expressions	1605:1632	expressions	1622:1632	01) and significantly inhibit the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions(P<0.					
30989865	8	20	theme	total	1049:1053	arg1	protein					1065:1071	phosphorylated and total PI3 K/AKT protein	1030:1071	phosphorylated and total PI3 K/AKT protein	1030:1071	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	20	21	theme	anti-inflammation	2373:2389	arg1	composition					2400:2410	its effective anti-inflammation chemical composition	2359:2410	its effective anti-inflammation chemical composition	2359:2410	In addition,emblin may be its effective anti-inflammation chemical composition.					
30989865	7	22	theme	CHUK	943:946	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	23	theme	fraction	285:292	arg1	A					294:294	Jingfang n-butanol extraction(JFNE) isolated fraction A	240:294	Jingfang n-butanol extraction(JFNE) isolated fraction A	240:294	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	8	24	theme	K/AKT	1059:1063	arg1	protein					1065:1071	phosphorylated and total PI3 K/AKT protein	1030:1071	phosphorylated and total PI3 K/AKT protein	1030:1071	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	0	25	from	inflammation	77:88	arg1	cells					102:106	RAW264.7 cells	93:106	RAW264.7 cells	93:106	[Effects of Jingfang n-butanol extraction isolated fraction A on LPS-induced inflammation in RAW264.7 cells].					
30989865	16	26	from	levels	1706:1711	arg1	RAW264					1793:1798	RAW264	1793:1798	RAW264	1793:1798	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	8	27	theme	p105	1093:1096	arg1	protein					1098:1104	p105 protein	1093:1104	p105 protein	1093:1104	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	8	27	theme	p105	1093:1096	arg1	p50					1079:1081	NF-κB p50	1073:1081	NF-κB p50	1073:1081	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	10	28	theme	IL-6	1401:1404	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	28	theme	IL-6	1401:1404	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	28	theme	IL-6	1401:1404	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	28	theme	IL-6	1401:1404	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	28	theme	IL-6	1401:1404	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	7	29	theme	AKT	939:941	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	30	theme	anti-inflammation	211:227	arg1	effects					229:235	the in vitro anti-inflammation effects	198:235	the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A	198:294	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	4	31	theme	cells	451:455	arg1	model					470:474	7 cells inflammation model	449:474	7 cells inflammation model	449:474	7 cells inflammation model was established by stimulating with LPS for 12 h.					
30989865	7	32	theme	NOS	929:931	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	33	theme	expression	1387:1396	arg1	levels					1326:1331	the release levels	1314:1331	the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1314:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	7	34	theme	K	937:937	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	35	theme	in	202:203	arg1	effects					229:235	the in vitro anti-inflammation effects	198:235	the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A	198:294	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	1	36	theme	LPS-induced	114:124	arg1	RAW264					126:131	The LPS-induced RAW264	110:131	The LPS-induced RAW264	110:131	The LPS-induced RAW264.					
30989865	17	37	dep	gene.A	1845:1850	arg1	identified					1880:1889	identified	1880:1889	were identified from fraction A in the composition analysis	1875:1933	7 cells,and increase the expression of CHUK gene.A total of 196 compounds were identified from fraction A in the composition analysis,and isoobtusilactone,5-O-methyl-vismitol,emebel(embelin) and prim-O-glucosylcimifugin showed high contents.					
30989865	17	38	theme	fraction	1896:1903	arg1	A					1905:1905	fraction A	1896:1905	fraction A	1896:1905	7 cells,and increase the expression of CHUK gene.A total of 196 compounds were identified from fraction A in the composition analysis,and isoobtusilactone,5-O-methyl-vismitol,emebel(embelin) and prim-O-glucosylcimifugin showed high contents.					
30989865	13	39	theme	K	1595:1595	arg1	levels					1581:1586	the phosphorylation expression levels	1550:1586	the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions	1550:1632	01) and significantly inhibit the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions(P<0.					
30989865	19	40	theme	model	2164:2168	arg1	cells					2170:2174	7 inflammation model cells	2149:2174	7 inflammation model cells	2149:2174	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	2	41	theme	n-butanol	249:257	arg1	JFNE					270:273	JFNE	270:273	JFNE	270:273	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	2	41	theme	n-butanol	249:257	arg1	extraction					259:268	Jingfang n-butanol extraction	240:268	Jingfang n-butanol extraction(JFNE) isolated fraction A	240:294	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	10	42	from	mRNA	1461:1464	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	43	theme	NOS	1426:1428	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	43	theme	NOS	1426:1428	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	43	theme	NOS	1426:1428	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	43	theme	NOS	1426:1428	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	43	theme	NOS	1426:1428	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	17	44	theme	compounds	1865:1873	arg1	total					1852:1856	total	1852:1856	total of 196 compounds	1852:1873	7 cells,and increase the expression of CHUK gene.A total of 196 compounds were identified from fraction A in the composition analysis,and isoobtusilactone,5-O-methyl-vismitol,emebel(embelin) and prim-O-glucosylcimifugin showed high contents.					
30989865	13	45	theme	AKT	1601:1603	arg1	levels					1581:1586	the phosphorylation expression levels	1550:1586	the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions	1550:1632	01) and significantly inhibit the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions(P<0.					
30989865	10	46	from	RAW264	1469:1474	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	46	from	RAW264	1469:1474	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	46	from	RAW264	1469:1474	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	46	from	RAW264	1469:1474	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	46	from	RAW264	1469:1474	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	16	47	theme	p50	1722:1724	arg1	levels					1706:1711	the levels	1702:1711	the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264	1702:1798	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	13	48	theme	expression	1570:1579	arg1	levels					1581:1586	the phosphorylation expression levels	1550:1586	the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions	1550:1632	01) and significantly inhibit the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions(P<0.					
30989865	18	49	theme	antiinflammatory	2102:2117	arg1	effect					2119:2124	a certain antiinflammatory effect	2092:2124	a certain antiinflammatory effect	2092:2124	The results all above showed that fraction A had a certain antiinflammatory effect in LPS-induced RAW264.					
30989865	16	50	theme	p-p65	1726:1730	arg1	levels					1706:1711	the levels	1702:1711	the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264	1702:1798	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	0	51	theme	RAW264.7	93:100	arg1	cells					102:106	RAW264.7 cells	93:106	RAW264.7 cells	93:106	[Effects of Jingfang n-butanol extraction isolated fraction A on LPS-induced inflammation in RAW264.7 cells].					
30989865	5	52	theme	interleukin-6	582:594	arg1	A					564:564	fraction A	555:564	fraction A	555:564	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	5	52	theme	interleukin-6	582:594	arg1	contents					570:577	the contents	566:577	the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264	566:684	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	10	53	from	levels	1326:1331	arg1	supernatant					1367:1377	the supernatant	1363:1377	the supernatant	1363:1377	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	17	54	theme	gene.A	1845:1850	arg1	expression					1826:1835	the expression	1822:1835	the expression of CHUK gene.A total of 196 compounds were identified from fraction A in the composition analysis	1822:1933	7 cells,and increase the expression of CHUK gene.A total of 196 compounds were identified from fraction A in the composition analysis,and isoobtusilactone,5-O-methyl-vismitol,emebel(embelin) and prim-O-glucosylcimifugin showed high contents.					
30989865	10	55	theme	fraction	1276:1283	arg1	A					1285:1285	fraction A	1276:1285	fraction A	1276:1285	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	56	theme	K/AKT	394:398	arg1	pathway					410:416	PI3 K/AKT signaling pathway	390:416	PI3 K/AKT signaling pathway	390:416	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	5	57	theme	fraction	555:562	arg1	A					564:564	fraction A	555:564	fraction A	555:564	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	5	57	theme	fraction	555:562	arg1	contents					570:577	the contents	566:577	the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264	566:684	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	16	58	theme	protein	1742:1748	arg1	levels					1706:1711	the levels	1702:1711	the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264	1702:1798	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	19	59	theme	K/AKT	2275:2279	arg1	pathway					2291:2297	PI3 K/AKT signaling pathway	2271:2297	PI3 K/AKT signaling pathway	2271:2297	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	7	60	used	used	868:871	arg2	method					857:862	Reverse transcription-polymerase chain reaction(RT-PCR) method	801:862	Reverse transcription-polymerase chain reaction(RT-PCR) method	801:862	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	6	61	from	contents	747:754	arg1	supernatant					765:775	supernatant	765:775	supernatant	765:775	7 cells inflammation model were determined by ELISA and the contents of NO in supernatant were assayed by Griess.					
30989865	13	62	dep	K	1595:1595	arg1	expressions					1622:1632	protein and mRNA expressions	1605:1632	expressions	1622:1632	01) and significantly inhibit the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions(P<0.					
30989865	2	63	theme	NF-κB	422:426	arg1	pathway					428:434	NF-κB pathway	422:434	NF-κB pathway	422:434	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	0	64	theme	Jingfang	12:19	arg1	extraction					31:40	Jingfang n-butanol extraction	12:40	Jingfang n-butanol extraction	12:40	[Effects of Jingfang n-butanol extraction isolated fraction A on LPS-induced inflammation in RAW264.7 cells].					
30989865	20	65	theme	chemical	2391:2398	arg1	composition					2400:2410	its effective anti-inflammation chemical composition	2359:2410	its effective anti-inflammation chemical composition	2359:2410	In addition,emblin may be its effective anti-inflammation chemical composition.					
30989865	0	66	theme	extraction	31:40	arg1	[Effects					0:7	[Effects	0:7	[Effects of Jingfang n-butanol extraction	0:40	[Effects of Jingfang n-butanol extraction isolated fraction A on LPS-induced inflammation in RAW264.7 cells].					
30989865	19	67	theme	NF-kappa	2303:2310	arg1	B					2312:2312	NF-kappa B	2303:2312	NF-kappa B signaling pathway	2303:2330	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	13	68	dep	inhibit	1542:1548	arg1	P<0					1634:1636	P<0	1634:1636	P<0	1634:1636	01) and significantly inhibit the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions(P<0.					
30989865	8	69	theme	expression	1009:1018	arg1	p50					1079:1081	NF-κB p50	1073:1081	NF-κB p50	1073:1081	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	8	69	theme	expression	1009:1018	arg1	levels					1020:1025	the expression levels	1005:1025	the expression levels of phosphorylated and total PI3 K/AKT protein	1005:1071	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	18	70	theme	fraction	2077:2084	arg1	A					2086:2086	fraction A	2077:2086	fraction A	2077:2086	The results all above showed that fraction A had a certain antiinflammatory effect in LPS-induced RAW264.					
30989865	6	71	theme	inflammation	695:706	arg1	model					708:712	7 cells inflammation model	687:712	7 cells inflammation model	687:712	7 cells inflammation model were determined by ELISA and the contents of NO in supernatant were assayed by Griess.					
30989865	0	72	theme	fraction	51:58	arg1	A					60:60	fraction A	51:60	fraction A	51:60	[Effects of Jingfang n-butanol extraction isolated fraction A on LPS-induced inflammation in RAW264.7 cells].					
30989865	8	73	from	levels	1020:1025	arg1	cells					1109:1113	cells	1109:1113	cells	1109:1113	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	19	74	theme	pathway	2291:2297	arg1	activation					2257:2266	the activation	2253:2266	the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway	2253:2330	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	10	75	theme	release	1318:1324	arg1	levels					1326:1331	the release levels	1314:1331	the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1314:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	76	theme	preliminary	312:322	arg1	mechanism					342:350	its preliminary anti-inflammation mechanism	308:350	its preliminary anti-inflammation mechanism	308:350	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	17	77	theme	composition	1914:1924	arg1	analysis					1926:1933	the composition analysis	1910:1933	the composition analysis	1910:1933	7 cells,and increase the expression of CHUK gene.A total of 196 compounds were identified from fraction A in the composition analysis,and isoobtusilactone,5-O-methyl-vismitol,emebel(embelin) and prim-O-glucosylcimifugin showed high contents.					
30989865	10	78	theme	IFN-γ	1418:1422	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	78	theme	IFN-γ	1418:1422	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	78	theme	IFN-γ	1418:1422	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	78	theme	IFN-γ	1418:1422	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	78	theme	IFN-γ	1418:1422	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	79	theme	regulatory	369:378	arg1	effect					380:385	the regulatory effect	365:385	the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway	365:434	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	10	80	from	supernatant	1367:1377	arg1	levels					1326:1331	the release levels	1314:1331	the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1314:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	19	81	theme	signaling	2314:2322	arg1	pathway					2324:2330	NF-kappa B signaling pathway	2303:2330	NF-kappa B signaling pathway	2303:2330	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	10	82	from	NOS	1426:1428	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	83	from	NO	1336:1337	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	83	from	NO	1336:1337	arg1	supernatant					1367:1377	the supernatant	1363:1377	the supernatant	1363:1377	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	7	84	theme	reaction	840:847	arg1	method					857:862	Reverse transcription-polymerase chain reaction(RT-PCR) method	801:862	Reverse transcription-polymerase chain reaction(RT-PCR) method	801:862	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	9	85	theme	possible	1206:1213	arg1	constituents					1224:1235	the possible chemical constituents	1202:1235	the possible chemical constituents in fraction A	1202:1249	In addition,LC-MS and database were used to identify the possible chemical constituents in fraction A.					
30989865	17	86	dep	cells	1803:1807	arg1	increase					1813:1820	increase	1813:1820	increase the expression of CHUK gene.A total of 196 compounds were identified from fraction A in the composition analysis	1813:1933	7 cells,and increase the expression of CHUK gene.A total of 196 compounds were identified from fraction A in the composition analysis,and isoobtusilactone,5-O-methyl-vismitol,emebel(embelin) and prim-O-glucosylcimifugin showed high contents.					
30989865	7	87	theme	mRNA	964:967	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	88	theme	isolated	276:283	arg1	A					294:294	Jingfang n-butanol extraction(JFNE) isolated fraction A	240:294	Jingfang n-butanol extraction(JFNE) isolated fraction A	240:294	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	7	89	theme	transcription-polymerase	809:832	arg1	RT-PCR					849:854	RT-PCR	849:854	RT-PCR	849:854	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	7	89	theme	transcription-polymerase	809:832	arg1	reaction					840:847	Reverse transcription-polymerase chain reaction	801:847	Reverse transcription-polymerase chain reaction(RT-PCR) method	801:862	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	90	from	IL-1β	1406:1410	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	5	91	dep	pre-treatment	536:548	arg1	h					534:534	3 h	532:534	3 h	532:534	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	2	92	theme	inflammation	142:153	arg1	carrier					175:181	a carrier	173:181	a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway	173:434	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	2	92	theme	inflammation	142:153	arg1	model					155:159	7 cells inflammation model	134:159	7 cells inflammation model	134:159	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	20	93	theme	effective	2363:2371	arg1	composition					2400:2410	its effective anti-inflammation chemical composition	2359:2410	its effective anti-inflammation chemical composition	2359:2410	In addition,emblin may be its effective anti-inflammation chemical composition.					
30989865	19	94	from	effect	2243:2248	arg1	activation					2257:2266	the activation	2253:2266	the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway	2253:2330	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	9	95	theme	fraction	1240:1247	arg1	A					1249:1249	fraction A	1240:1249	fraction A	1240:1249	In addition,LC-MS and database were used to identify the possible chemical constituents in fraction A.					
30989865	9	96	used	used	1185:1188	arg2	database					1171:1178	database	1171:1178	database	1171:1178	In addition,LC-MS and database were used to identify the possible chemical constituents in fraction A.					
30989865	9	96	used	used	1185:1188	arg2	LC-MS					1161:1165	LC-MS	1161:1165	LC-MS	1161:1165	In addition,LC-MS and database were used to identify the possible chemical constituents in fraction A.					
30989865	8	97	theme	NF-κB	1073:1077	arg1	p-p65					1087:1091	p-p65	1087:1091	p-p65	1087:1091	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	8	97	theme	NF-κB	1073:1077	arg1	protein					1098:1104	p105 protein	1093:1104	p105 protein	1093:1104	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	8	97	theme	NF-κB	1073:1077	arg1	p65					1083:1085	p65	1083:1085	p65	1083:1085	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	8	97	theme	NF-κB	1073:1077	arg1	p50					1079:1081	NF-κB p50	1073:1081	NF-κB p50	1073:1081	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	8	97	theme	NF-κB	1073:1077	arg1	levels					1020:1025	the expression levels	1005:1025	the expression levels of phosphorylated and total PI3 K/AKT protein	1005:1071	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	10	98	theme	mRNA	1461:1464	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	98	theme	mRNA	1461:1464	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	98	theme	mRNA	1461:1464	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	98	theme	mRNA	1461:1464	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	98	theme	mRNA	1461:1464	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	16	99	dep	p50	1722:1724	arg1	expressions					1778:1788	Rela mRNA expressions	1768:1788	Rela mRNA expressions	1768:1788	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	16	100	theme	NOS	1738:1740	arg1	protein					1742:1748	i NOS protein	1736:1748	i NOS protein	1736:1748	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	7	101	theme	NF-κB1	948:953	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	102	theme	A	294:294	arg1	effects					229:235	the in vitro anti-inflammation effects	198:235	the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A	198:294	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	2	103	dep	in	202:203	arg1	vitro					205:209	vitro	205:209	vitro	205:209	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	10	104	theme	K	1434:1434	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	104	theme	K	1434:1434	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	104	theme	K	1434:1434	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	104	theme	K	1434:1434	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	104	theme	K	1434:1434	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	19	105	theme	inflammation	2151:2162	arg1	cells					2170:2174	7 inflammation model cells	2149:2174	7 inflammation model cells	2149:2174	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	8	106	theme	PI3	1055:1057	arg1	protein					1065:1071	phosphorylated and total PI3 K/AKT protein	1030:1071	phosphorylated and total PI3 K/AKT protein	1030:1071	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	4	107	theme	inflammation	457:468	arg1	model					470:474	7 cells inflammation model	449:474	7 cells inflammation model	449:474	7 cells inflammation model was established by stimulating with LPS for 12 h.					
30989865	16	108	theme	mRNA	1773:1776	arg1	expressions					1778:1788	Rela mRNA expressions	1768:1788	Rela mRNA expressions	1768:1788	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	7	109	theme	PI3	933:935	arg1	K					937:937	PI3 K	933:937	PI3 K	933:937	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	8	110	theme	protein	1065:1071	arg1	cells					995:999	7 inflammatory cells	980:999	7 inflammatory cells	980:999	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	8	110	theme	protein	1065:1071	arg1	p50					1079:1081	NF-κB p50	1073:1081	NF-κB p50	1073:1081	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	8	110	theme	protein	1065:1071	arg1	levels					1020:1025	the expression levels	1005:1025	the expression levels of phosphorylated and total PI3 K/AKT protein	1005:1071	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	13	111	theme	PI3	1591:1593	arg1	K					1595:1595	PI3 K	1591:1595	PI3 K	1591:1595	01) and significantly inhibit the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions(P<0.					
30989865	5	112	theme	factor	643:648	arg1	A					564:564	fraction A	555:564	fraction A	555:564	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	5	112	theme	factor	643:648	arg1	contents					570:577	the contents	566:577	the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264	566:684	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	2	113	theme	extraction	259:268	arg1	A					294:294	Jingfang n-butanol extraction(JFNE) isolated fraction A	240:294	Jingfang n-butanol extraction(JFNE) isolated fraction A	240:294	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	7	114	theme	IFN-γ	921:925	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	115	from	NF-κB1	1445:1450	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	116	theme	PI3	1430:1432	arg1	K					1434:1434	PI3 K	1430:1434	PI3 K	1430:1434	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	0	117	theme	LPS-induced	65:75	arg1	inflammation					77:88	LPS-induced inflammation	65:88	LPS-induced inflammation in RAW264.7 cells	65:106	[Effects of Jingfang n-butanol extraction isolated fraction A on LPS-induced inflammation in RAW264.7 cells].					
30989865	2	118	theme	Jingfang	240:247	arg1	JFNE					270:273	JFNE	270:273	JFNE	270:273	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	2	118	theme	Jingfang	240:247	arg1	extraction					259:268	Jingfang n-butanol extraction	240:268	Jingfang n-butanol extraction(JFNE) isolated fraction A	240:294	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	7	119	theme	TNF-α	915:919	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	7	120	from	expression	890:899	arg1	RAW264					972:977	RAW264	972:977	RAW264	972:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	13	121	theme	phosphorylation	1554:1568	arg1	levels					1581:1586	the phosphorylation expression levels	1550:1586	the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions	1550:1632	01) and significantly inhibit the phosphorylation expression levels of PI3 K and AKT protein and mRNA expressions(P<0.					
30989865	7	122	theme	IL-1β	909:913	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	5	123	from	contents	570:577	arg1	supernatant					664:674	the supernatant	660:674	the supernatant of RAW264	660:684	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	5	124	with	pre-treatment	536:548	arg1	A					564:564	fraction A	555:564	fraction A	555:564	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	5	124	with	pre-treatment	536:548	arg1	contents					570:577	the contents	566:577	the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264	566:684	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	16	125	theme	NF-κB	1716:1720	arg1	p50					1722:1724	NF-κB p50	1716:1724	NF-κB p50	1716:1724	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	8	126	theme	Western	1135:1141	arg1	blot					1143:1146	Western blot	1135:1146	Western blot	1135:1146	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	19	127	theme	anti-inflammatory	2184:2200	arg1	effects					2202:2208	its anti-inflammatory effects	2180:2208	its anti-inflammatory effects	2180:2208	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	7	128	theme	IL-6	904:907	arg1	expression					890:899	the expression	886:899	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	886:977	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	2	129	used	used	165:168	arg2	model					155:159	7 cells inflammation model	134:159	7 cells inflammation model	134:159	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	2	129	used	used	165:168	arg2	carrier					175:181	a carrier	173:181	a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway	173:434	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	5	130	theme	RAW264	679:684	arg1	supernatant					664:674	the supernatant	660:674	the supernatant of RAW264	660:684	After 3 h pre-treatment with fraction A,the contents of interleukin-6(IL-6),interleukin-1β(IL-1β) and tumor necrosis factor(TNF-α) in the supernatant of RAW264.					
30989865	20	131	from	composition	2400:2410	arg1	addition					2336:2343	addition	2336:2343	addition	2336:2343	In addition,emblin may be its effective anti-inflammation chemical composition.					
30989865	19	132	theme	regulatory	2232:2241	arg1	effect					2243:2248	its regulatory effect	2228:2248	its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway	2228:2330	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	16	133	theme	Rela	1768:1771	arg1	expressions					1778:1788	Rela mRNA expressions	1768:1788	Rela mRNA expressions	1768:1788	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	2	134	theme	PI3	390:392	arg1	pathway					410:416	PI3 K/AKT signaling pathway	390:416	PI3 K/AKT signaling pathway	390:416	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	19	135	theme	PI3	2271:2273	arg1	pathway					2291:2297	PI3 K/AKT signaling pathway	2271:2297	PI3 K/AKT signaling pathway	2271:2297	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	10	136	from	TNF-α	1354:1358	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	136	from	TNF-α	1354:1358	arg1	supernatant					1367:1377	the supernatant	1363:1377	the supernatant	1363:1377	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	16	137	theme	i	1736:1736	arg1	protein					1742:1748	i NOS protein	1736:1748	i NOS protein	1736:1748	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	10	138	theme	IL-1β	1406:1410	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	138	theme	IL-1β	1406:1410	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	138	theme	IL-1β	1406:1410	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	138	theme	IL-1β	1406:1410	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	138	theme	IL-1β	1406:1410	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	0	139	theme	n-butanol	21:29	arg1	extraction					31:40	Jingfang n-butanol extraction	12:40	Jingfang n-butanol extraction	12:40	[Effects of Jingfang n-butanol extraction isolated fraction A on LPS-induced inflammation in RAW264.7 cells].					
30989865	9	140	from	constituents	1224:1235	arg1	A					1249:1249	fraction A	1240:1249	fraction A	1240:1249	In addition,LC-MS and database were used to identify the possible chemical constituents in fraction A.					
30989865	16	141	theme	fraction	1664:1671	arg1	A					1673:1673	fraction A	1664:1673	fraction A	1664:1673	Moreover,fraction A could significantly reduce the levels of NF-κB p50,p-p65 and i NOS protein,as well as NF-κB1,Rela mRNA expressions in RAW264.					
30989865	18	142	contain	had	2088:2090	arg1	A					2086:2086	fraction A	2077:2086	fraction A	2077:2086	The results all above showed that fraction A had a certain antiinflammatory effect in LPS-induced RAW264.					
30989865	18	142	contain	had	2088:2090	arg2	effect					2119:2124	a certain antiinflammatory effect	2092:2124	a certain antiinflammatory effect	2092:2124	The results all above showed that fraction A had a certain antiinflammatory effect in LPS-induced RAW264.					
30989865	8	143	from	cells	995:999	arg1	cells					1109:1113	cells	1109:1113	cells	1109:1113	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	18	144	theme	LPS-induced	2129:2139	arg1	RAW264					2141:2146	LPS-induced RAW264	2129:2146	LPS-induced RAW264	2129:2146	The results all above showed that fraction A had a certain antiinflammatory effect in LPS-induced RAW264.					
30989865	2	145	theme	signaling	400:408	arg1	pathway					410:416	PI3 K/AKT signaling pathway	390:416	PI3 K/AKT signaling pathway	390:416	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	10	146	from	IL-6	1339:1342	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	146	from	IL-6	1339:1342	arg1	supernatant					1367:1377	the supernatant	1363:1377	the supernatant	1363:1377	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	19	147	theme	signaling	2281:2289	arg1	pathway					2291:2297	PI3 K/AKT signaling pathway	2271:2297	PI3 K/AKT signaling pathway	2271:2297	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	2	148	from	effect	380:385	arg1	pathway					410:416	PI3 K/AKT signaling pathway	390:416	PI3 K/AKT signaling pathway	390:416	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	2	148	from	effect	380:385	arg1	pathway					428:434	NF-κB pathway	422:434	NF-κB pathway	422:434	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	17	149	theme	high	2028:2031	arg1	contents					2033:2040	high contents	2028:2040	high contents	2028:2040	7 cells,and increase the expression of CHUK gene.A total of 196 compounds were identified from fraction A in the composition analysis,and isoobtusilactone,5-O-methyl-vismitol,emebel(embelin) and prim-O-glucosylcimifugin showed high contents.					
30989865	6	150	theme	cells	689:693	arg1	model					708:712	7 cells inflammation model	687:712	7 cells inflammation model	687:712	7 cells inflammation model were determined by ELISA and the contents of NO in supernatant were assayed by Griess.					
30989865	2	151	theme	anti-inflammation	324:340	arg1	mechanism					342:350	its preliminary anti-inflammation mechanism	308:350	its preliminary anti-inflammation mechanism	308:350	7 cells inflammation model was used as a carrier to investigate the in vitro anti-inflammation effects of Jingfang n-butanol extraction(JFNE) isolated fraction A and explore its preliminary anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT signaling pathway and NF-κB pathway.					
30989865	10	152	from	IL-1β	1344:1348	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	152	from	IL-1β	1344:1348	arg1	supernatant					1367:1377	the supernatant	1363:1377	the supernatant	1363:1377	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	19	153	theme	pathway	2324:2330	arg1	activation					2257:2266	the activation	2253:2266	the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway	2253:2330	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	10	154	from	TNF-α	1412:1416	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	8	155	theme	inflammatory	982:993	arg1	cells					995:999	7 inflammatory cells	980:999	7 inflammatory cells	980:999	7 inflammatory cells,and the expression levels of phosphorylated and total PI3 K/AKT protein,NF-κB p50,p65,p-p65,p105 protein in cells were determined via Western blot.					
30989865	6	156	theme	NO	759:760	arg1	contents					747:754	the contents	743:754	the contents of NO in supernatant	743:775	7 cells inflammation model were determined by ELISA and the contents of NO in supernatant were assayed by Griess.					
30989865	19	157	theme	B	2312:2312	arg1	pathway					2324:2330	NF-kappa B signaling pathway	2303:2330	NF-kappa B signaling pathway	2303:2330	7 inflammation model cells,and its anti-inflammatory effects may be related to its regulatory effect on the activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway.					
30989865	10	158	from	IFN-γ	1418:1422	arg1	RAW264					1469:1474	RAW264	1469:1474	RAW264	1469:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	159	theme	TNF-α	1412:1416	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	159	theme	TNF-α	1412:1416	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	159	theme	TNF-α	1412:1416	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	159	theme	TNF-α	1412:1416	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	159	theme	TNF-α	1412:1416	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	160	theme	IL-6	1339:1342	arg1	levels					1326:1331	the release levels	1314:1331	the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1314:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	161	theme	CHUK	1440:1443	arg1	IL-6					1339:1342	IL-6	1339:1342	IL-6	1339:1342	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	161	theme	CHUK	1440:1443	arg1	NO					1336:1337	NO	1336:1337	NO	1336:1337	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	161	theme	CHUK	1440:1443	arg1	TNF-α					1354:1358	TNF-α	1354:1358	TNF-α	1354:1358	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	161	theme	CHUK	1440:1443	arg1	IL-1β					1344:1348	IL-1β	1344:1348	IL-1β	1344:1348	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	10	161	theme	CHUK	1440:1443	arg1	expression					1387:1396	the expression	1383:1396	the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264	1383:1474	The results showed that fraction A could significantly reduce the release levels of NO,IL-6,IL-1β and TNF-α in the supernatant and the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	7	162	theme	chain	834:838	arg1	RT-PCR					849:854	RT-PCR	849:854	RT-PCR	849:854	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
30989865	7	162	theme	chain	834:838	arg1	reaction					840:847	Reverse transcription-polymerase chain reaction	801:847	Reverse transcription-polymerase chain reaction(RT-PCR) method	801:862	Reverse transcription-polymerase chain reaction(RT-PCR) method was used to determine the expression of IL-6,IL-1β,TNF-α,IFN-γ,i NOS,PI3 K,AKT,CHUK,NF-κB1 and Rela mRNA in RAW264.					
29603240	8	0	theme	fillets	976:982	arg1	qualities					957:965	The sensory qualities	945:965	The sensory qualities of these fillets	945:982	The sensory qualities of these fillets were effectively maintained.					
29603240	7	1	theme	IG	858:859	arg1	chitosan					823:830	5 g L-1 chitosan	815:830	5 g L-1 chitosan	815:830	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	1	theme	IG	858:859	arg1	layers					861:866	IG layers	858:866	IG layers	858:866	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	1	theme	IG	858:859	arg1	catechin					846:853	1∼3 g L-1 catechin	836:853	1∼3 g L-1 catechin	836:853	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	1	2	theme	microbial	258:266	arg1	spoilage					268:275	microbial spoilage	258:275	microbial spoilage	258:275	BACKGROUND Most aquatic products are highly susceptible to deterioration and microbial spoilage during storage.					
29603240	0	3	theme	tilapia	172:178	arg1	spoilage					128:135	microbiological spoilage	112:135	microbiological spoilage	112:135	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	0	3	theme	tilapia	172:178	arg1	oxidation					149:157	organic oxidation	141:157	organic oxidation	141:157	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	7	4	theme	fillets	936:942	arg1	quality					907:913	the postmortem quality	892:913	the postmortem quality of preserved tilapia fillets	892:942	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	5	theme	L-1	842:844	arg1	chitosan					823:830	5 g L-1 chitosan	815:830	5 g L-1 chitosan	815:830	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	5	theme	L-1	842:844	arg1	catechin					846:853	1∼3 g L-1 catechin	836:853	1∼3 g L-1 catechin	836:853	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	5	theme	L-1	842:844	arg1	layers					861:866	IG layers	858:866	IG layers	858:866	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	0	6	theme	composite	84:92	arg1	hydrogel					94:101	biopolymer-based composite hydrogel	67:101	biopolymer-based composite hydrogel	67:101	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	7	7	theme	preserved	918:926	arg1	fillets					936:942	preserved tilapia fillets	918:942	preserved tilapia fillets	918:942	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	0	8	theme	biopolymer-based	67:82	arg1	hydrogel					94:101	biopolymer-based composite hydrogel	67:101	biopolymer-based composite hydrogel	67:101	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	5	9	theme	chitosan-catechin	605:621	arg1	material					633:640	chitosan-catechin composite material	605:640	chitosan-catechin composite material	605:640	To solve the problem, this work established a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material.					
29603240	7	10	theme	1∼3	836:838	arg1	chitosan					823:830	5 g L-1 chitosan	815:830	5 g L-1 chitosan	815:830	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	10	theme	1∼3	836:838	arg1	catechin					846:853	1∼3 g L-1 catechin	836:853	1∼3 g L-1 catechin	836:853	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	10	theme	1∼3	836:838	arg1	layers					861:866	IG layers	858:866	IG layers	858:866	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	12	11	theme	chitosan-catechin	1244:1260	arg1	layers					1284:1289	chitosan-catechin composite-ice glazing layers	1244:1289	chitosan-catechin composite-ice glazing layers	1244:1289	CONCLUSION The results indicated that the SS-IG with chitosan-catechin composite-ice glazing layers can be effective in maintaining the postmortem quality of tilapia fillets.					
29603240	10	12	theme	Oxidative	1077:1085	arg1	rancidity					1087:1095	Oxidative rancidity	1077:1095	Oxidative rancidity in these fillets	1077:1112	Oxidative rancidity in these fillets was observably inhibited.					
29603240	5	13	theme	composite	623:631	arg1	material					633:640	chitosan-catechin composite material	605:640	chitosan-catechin composite material	605:640	To solve the problem, this work established a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material.					
29603240	2	14	theme	Cold	293:296	arg1	storage					298:304	Cold storage	293:304	Cold storage	293:304	Cold storage is a frequently used method to preserve them.					
29603240	2	14	theme	Cold	293:296	arg1	method					327:332	a frequently used method	309:332	a frequently used method to preserve them	309:349	Cold storage is a frequently used method to preserve them.					
29603240	7	15	theme	g	840:840	arg1	chitosan					823:830	5 g L-1 chitosan	815:830	5 g L-1 chitosan	815:830	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	15	theme	g	840:840	arg1	catechin					846:853	1∼3 g L-1 catechin	836:853	1∼3 g L-1 catechin	836:853	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	15	theme	g	840:840	arg1	layers					861:866	IG layers	858:866	IG layers	858:866	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	8	16	theme	sensory	949:955	arg1	qualities					957:965	The sensory qualities	945:965	The sensory qualities of these fillets	945:982	The sensory qualities of these fillets were effectively maintained.					
29603240	5	17	theme	glazing	574:580	arg1	approach					590:597	a novel superchilling storage-ice glazing (SS-IG) approach	540:597	a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material	540:640	To solve the problem, this work established a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material.					
29603240	3	18	theme	frozen	395:400	arg1	method					402:407	traditional frozen method	383:407	traditional frozen method	383:407	However, products preserved by traditional frozen method are prone to suffer damage.					
29603240	4	19	theme	products	486:493	arg1	quality					471:477	the quality	467:477	the quality of the products	467:493	This can significantly impair the quality of the products.					
29603240	12	20	dep	CONCLUSION	1191:1200	arg1	indicated					1214:1222	indicated	1214:1222	indicated that the SS-IG with chitosan-catechin composite-ice glazing layers can be effective in maintaining the postmortem quality of tilapia fillets	1214:1363	CONCLUSION The results indicated that the SS-IG with chitosan-catechin composite-ice glazing layers can be effective in maintaining the postmortem quality of tilapia fillets.					
29603240	1	21	theme	BACKGROUND	181:190	arg1	products					205:212	BACKGROUND Most aquatic products	181:212	BACKGROUND Most aquatic products	181:212	BACKGROUND Most aquatic products are highly susceptible to deterioration and microbial spoilage during storage.					
29603240	7	22	theme	SS-IG	794:798	arg1	approach					800:807	the SS-IG approach	790:807	the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers	790:866	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	5	23	theme	SS-IG	583:587	arg1	approach					590:597	a novel superchilling storage-ice glazing (SS-IG) approach	540:597	a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material	540:640	To solve the problem, this work established a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material.					
29603240	2	24	theme	used	322:325	arg1	storage					298:304	Cold storage	293:304	Cold storage	293:304	Cold storage is a frequently used method to preserve them.					
29603240	2	24	theme	used	322:325	arg1	method					327:332	a frequently used method	309:332	a frequently used method to preserve them	309:349	Cold storage is a frequently used method to preserve them.					
29603240	7	25	theme	g	817:817	arg1	layers					861:866	IG layers	858:866	IG layers	858:866	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	25	theme	g	817:817	arg1	chitosan					823:830	5 g L-1 chitosan	815:830	5 g L-1 chitosan	815:830	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	25	theme	g	817:817	arg1	catechin					846:853	1∼3 g L-1 catechin	836:853	1∼3 g L-1 catechin	836:853	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	1	26	theme	Most	192:195	arg1	products					205:212	BACKGROUND Most aquatic products	181:212	BACKGROUND Most aquatic products	181:212	BACKGROUND Most aquatic products are highly susceptible to deterioration and microbial spoilage during storage.					
29603240	0	27	theme	storage	22:28	arg1	approach					52:59	A novel superchilling storage - ice glazing (SS-IG) approach	0:59	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel	0:101	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	0	28	theme	microbiological	112:126	arg1	spoilage					128:135	microbiological spoilage	112:135	microbiological spoilage	112:135	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	7	29	theme	postmortem	896:905	arg1	quality					907:913	the postmortem quality	892:913	the postmortem quality of preserved tilapia fillets	892:942	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	6	30	theme	products	695:702	arg1	quality					674:680	the postmortem quality	659:680	the postmortem quality of preserved products	659:702	It can maximize the postmortem quality of preserved products during storage, avoiding damage.					
29603240	1	31	theme	aquatic	197:203	arg1	products					205:212	BACKGROUND Most aquatic products	181:212	BACKGROUND Most aquatic products	181:212	BACKGROUND Most aquatic products are highly susceptible to deterioration and microbial spoilage during storage.					
29603240	3	32	theme	traditional	383:393	arg1	method					402:407	traditional frozen method	383:407	traditional frozen method	383:407	However, products preserved by traditional frozen method are prone to suffer damage.					
29603240	7	33	from	±	768:768	arg1	storage					752:758	storage	752:758	storage at -1.5 ± 0.2	752:772	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	6	34	theme	preserved	685:693	arg1	products					695:702	preserved products	685:702	preserved products	685:702	It can maximize the postmortem quality of preserved products during storage, avoiding damage.					
29603240	12	35	theme	glazing	1276:1282	arg1	layers					1284:1289	chitosan-catechin composite-ice glazing layers	1244:1289	chitosan-catechin composite-ice glazing layers	1244:1289	CONCLUSION The results indicated that the SS-IG with chitosan-catechin composite-ice glazing layers can be effective in maintaining the postmortem quality of tilapia fillets.					
29603240	0	36	theme	ice	32:34	arg1	approach					52:59	A novel superchilling storage - ice glazing (SS-IG) approach	0:59	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel	0:101	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	7	37	theme	tilapia	928:934	arg1	fillets					936:942	preserved tilapia fillets	918:942	preserved tilapia fillets	918:942	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	14	38	theme	Chemical	1429:1436	arg1	Industry					1438:1445	Chemical Industry	1429:1445	Chemical Industry	1429:1445	© 2018 Society of Chemical Industry.					
29603240	12	39	with	SS-IG	1233:1237	arg1	layers					1284:1289	chitosan-catechin composite-ice glazing layers	1244:1289	chitosan-catechin composite-ice glazing layers	1244:1289	CONCLUSION The results indicated that the SS-IG with chitosan-catechin composite-ice glazing layers can be effective in maintaining the postmortem quality of tilapia fillets.					
29603240	12	40	theme	fillets	1357:1363	arg1	quality					1338:1344	the postmortem quality	1323:1344	the postmortem quality of tilapia fillets	1323:1363	CONCLUSION The results indicated that the SS-IG with chitosan-catechin composite-ice glazing layers can be effective in maintaining the postmortem quality of tilapia fillets.					
29603240	13	41	theme	potential	1388:1396	arg1	application					1398:1408	a wide potential application	1381:1408	a wide potential application	1381:1408	This will have a wide potential application.					
29603240	10	42	from	rancidity	1087:1095	arg1	fillets					1106:1112	these fillets	1100:1112	these fillets	1100:1112	Oxidative rancidity in these fillets was observably inhibited.					
29603240	5	43	theme	novel	542:546	arg1	approach					590:597	a novel superchilling storage-ice glazing (SS-IG) approach	540:597	a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material	540:640	To solve the problem, this work established a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material.					
29603240	0	44	theme	glazing	36:42	arg1	approach					52:59	A novel superchilling storage - ice glazing (SS-IG) approach	0:59	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel	0:101	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	13	45	theme	wide	1383:1386	arg1	application					1398:1408	a wide potential application	1381:1408	a wide potential application	1381:1408	This will have a wide potential application.					
29603240	0	46	theme	organic	141:147	arg1	oxidation					149:157	organic oxidation	141:157	organic oxidation	141:157	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	5	47	theme	superchilling	548:560	arg1	approach					590:597	a novel superchilling storage-ice glazing (SS-IG) approach	540:597	a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material	540:640	To solve the problem, this work established a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material.					
29603240	13	48	contain	have	1376:1379	arg1	This					1366:1369	This	1366:1369	This	1366:1369	This will have a wide potential application.					
29603240	13	48	contain	have	1376:1379	arg2	application					1398:1408	a wide potential application	1381:1408	a wide potential application	1381:1408	This will have a wide potential application.					
29603240	0	49	theme	preserved	162:170	arg1	tilapia					172:178	preserved tilapia	162:178	preserved tilapia	162:178	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	7	50	dep	RESULTS	737:743	arg1	enhance					884:890	enhance	884:890	can effectively enhance the postmortem quality of preserved tilapia fillets	868:942	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	11	51	theme	biogenic	1145:1152	arg1	amine					1154:1158	Less biogenic amine	1140:1158	Less biogenic amine	1140:1158	Less biogenic amine was produced in these fillets.					
29603240	5	52	theme	storage-ice	562:572	arg1	approach					590:597	a novel superchilling storage-ice glazing (SS-IG) approach	540:597	a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material	540:640	To solve the problem, this work established a novel superchilling storage-ice glazing (SS-IG) approach using chitosan-catechin composite material.					
29603240	12	53	theme	composite-ice	1262:1274	arg1	layers					1284:1289	chitosan-catechin composite-ice glazing layers	1244:1289	chitosan-catechin composite-ice glazing layers	1244:1289	CONCLUSION The results indicated that the SS-IG with chitosan-catechin composite-ice glazing layers can be effective in maintaining the postmortem quality of tilapia fillets.					
29603240	6	54	theme	postmortem	663:672	arg1	quality					674:680	the postmortem quality	659:680	the postmortem quality of preserved products	659:702	It can maximize the postmortem quality of preserved products during storage, avoiding damage.					
29603240	9	55	from	counts	1027:1032	arg1	fillets					1043:1049	these fillets	1037:1049	these fillets	1037:1049	The microbial counts in these fillets were strongly suppressed.					
29603240	12	56	theme	postmortem	1327:1336	arg1	quality					1338:1344	the postmortem quality	1323:1344	the postmortem quality of tilapia fillets	1323:1363	CONCLUSION The results indicated that the SS-IG with chitosan-catechin composite-ice glazing layers can be effective in maintaining the postmortem quality of tilapia fillets.					
29603240	7	57	theme	L-1	819:821	arg1	layers					861:866	IG layers	858:866	IG layers	858:866	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	57	theme	L-1	819:821	arg1	chitosan					823:830	5 g L-1 chitosan	815:830	5 g L-1 chitosan	815:830	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	7	57	theme	L-1	819:821	arg1	catechin					846:853	1∼3 g L-1 catechin	836:853	1∼3 g L-1 catechin	836:853	RESULTS During storage at -1.5 ± 0.2 °C for 25 days, the SS-IG approach using 5 g L-1 chitosan and 1∼3 g L-1 catechin as IG layers can effectively enhance the postmortem quality of preserved tilapia fillets.					
29603240	12	58	theme	tilapia	1349:1355	arg1	fillets					1357:1363	tilapia fillets	1349:1363	tilapia fillets	1349:1363	CONCLUSION The results indicated that the SS-IG with chitosan-catechin composite-ice glazing layers can be effective in maintaining the postmortem quality of tilapia fillets.					
29603240	9	59	theme	microbial	1017:1025	arg1	counts					1027:1032	The microbial counts	1013:1032	The microbial counts in these fillets	1013:1049	The microbial counts in these fillets were strongly suppressed.					
29603240	0	60	theme	SS-IG	45:49	arg1	approach					52:59	A novel superchilling storage - ice glazing (SS-IG) approach	0:59	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel	0:101	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	0	61	theme	superchilling	8:20	arg1	approach					52:59	A novel superchilling storage - ice glazing (SS-IG) approach	0:59	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel	0:101	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
29603240	0	62	theme	novel	2:6	arg1	approach					52:59	A novel superchilling storage - ice glazing (SS-IG) approach	0:59	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel	0:101	A novel superchilling storage - ice glazing (SS-IG) approach using biopolymer-based composite hydrogel to delay microbiological spoilage and organic oxidation of preserved tilapia.					
30468509	0	0	from	prebiotics	12:21	arg1	era					95:97	the microbiome era	80:97	the microbiome era	80:97	Probiotics, prebiotics and synbiotics for weight loss and metabolic syndrome in the microbiome era.					
30468509	1	1	theme	associated	137:146	arg1	consequences					161:172	the associated dysmetabolic consequences	133:172	the associated dysmetabolic consequences	133:172	OBJECTIVE Excessive body fat and the associated dysmetabolic consequences affect both developed and emerging countries.					
30468509	3	2	theme	gut	341:343	arg1	composition					356:366	gut microbiome composition	341:366	gut microbiome composition	341:366	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	6	3	theme	prebiotic	1028:1036	arg1	compounds					1038:1046	selected probiotic and prebiotic compounds	1005:1046	selected probiotic and prebiotic compounds	1005:1046	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	10	4	theme	acid	1590:1593	arg1	production					1595:1604	short chain fatty acid production	1572:1604	short chain fatty acid production	1572:1604	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	1	5	theme	dysmetabolic	148:159	arg1	consequences					161:172	the associated dysmetabolic consequences	133:172	the associated dysmetabolic consequences	133:172	OBJECTIVE Excessive body fat and the associated dysmetabolic consequences affect both developed and emerging countries.					
30468509	6	6	dep	RESULTS	867:873	arg1	studies					883:889	Several studies	875:889	RESULTS Several studies in humans and in animal models	867:920	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	9	7	theme	large	1405:1409	arg1	studies					1423:1429	large independent studies	1405:1429	large independent studies	1405:1429	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	0	8	theme	microbiome	84:93	arg1	era					95:97	the microbiome era	80:97	the microbiome era	80:97	Probiotics, prebiotics and synbiotics for weight loss and metabolic syndrome in the microbiome era.					
30468509	0	9	from	Probiotics	0:9	arg1	era					95:97	the microbiome era	80:97	the microbiome era	80:97	Probiotics, prebiotics and synbiotics for weight loss and metabolic syndrome in the microbiome era.					
30468509	10	10	theme	short	1572:1576	arg1	acid					1590:1593	short chain fatty acid	1572:1593	short chain fatty acid production	1572:1604	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	6	11	theme	animal	908:913	arg1	models					915:920	animal models	908:920	animal models	908:920	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	4	12	theme	probiotics/prebiotics	633:653	arg1	terms					728:732	the search terms	717:732	the search terms	717:732	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	4	12	theme	probiotics/prebiotics	633:653	arg1	combinations					617:628	combinations	617:628	combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome	617:712	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	5	13	theme	clinical	829:836	arg1	results					838:844	clinical results	829:844	clinical results	829:844	Clinical studies and significant pre-clinical results showing molecular mechanisms supporting clinical results were also discussed.					
30468509	6	14	theme	probiotic	1014:1022	arg1	compounds					1038:1046	selected probiotic and prebiotic compounds	1005:1046	selected probiotic and prebiotic compounds	1005:1046	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	5	15	theme	Clinical	735:742	arg1	studies					744:750	Clinical studies	735:750	Clinical studies	735:750	Clinical studies and significant pre-clinical results showing molecular mechanisms supporting clinical results were also discussed.					
30468509	10	16	theme	chain	1578:1582	arg1	acid					1590:1593	short chain fatty acid	1572:1593	short chain fatty acid production	1572:1604	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	3	17	theme	dysmetabolic	521:532	arg1	complications					534:546	dysmetabolic complications	521:546	dysmetabolic complications	521:546	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	9	18	theme	CONCLUSIONS	1217:1227	arg1	supplementation					1234:1248	CONCLUSIONS Diet supplementation	1217:1248	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains)	1217:1336	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	6	19	theme	selected	1005:1012	arg1	compounds					1038:1046	selected probiotic and prebiotic compounds	1005:1046	selected probiotic and prebiotic compounds	1005:1046	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	8	20	theme	beneficial	1197:1206	arg1	effects					1208:1214	independent and synergistic beneficial effects	1169:1214	independent and synergistic beneficial effects	1169:1214	Fibers such as inulin or galactomannan promote independent and synergistic beneficial effects.					
30468509	4	21	theme	loss/metabolic	690:703	arg1	syndrome					705:712	obesity/weight loss/metabolic syndrome	675:712	obesity/weight loss/metabolic syndrome	675:712	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	9	22	theme	Diet	1229:1232	arg1	supplementation					1234:1248	CONCLUSIONS Diet supplementation	1217:1248	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains)	1217:1336	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	3	23	theme	promising	452:460	arg1	tool					462:465	a promising tool	450:465	a promising tool for excessive body weight treatment and prevention of dysmetabolic complications	450:546	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	0	24	theme	weight	42:47	arg1	loss					49:52	weight loss	42:52	weight loss	42:52	Probiotics, prebiotics and synbiotics for weight loss and metabolic syndrome in the microbiome era.					
30468509	8	25	theme	synergistic	1185:1195	arg1	effects					1208:1214	independent and synergistic beneficial effects	1169:1214	independent and synergistic beneficial effects	1169:1214	Fibers such as inulin or galactomannan promote independent and synergistic beneficial effects.					
30468509	10	26	theme	're	1621:1623	arg1	-configuration					1624:1637	microbiota 're-configuration	1610:1637	microbiota 're-configuration'	1610:1638	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	2	27	theme	environmental	274:286	arg1	cause					288:292	an important environmental cause	261:292	an important environmental cause of these conditions	261:312	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	2	27	theme	environmental	274:286	arg1	composition					246:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	9	28	dep	strains	1290:1296	arg1	such					1299:1302	such	1299:1302	such	1299:1302	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	4	29	theme	search	721:726	arg1	terms					728:732	the search terms	717:732	the search terms	717:732	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	4	29	theme	search	721:726	arg1	combinations					617:628	combinations	617:628	combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome	617:712	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	10	30	theme	management	1524:1533	arg1	effects					1535:1541	weight management effects	1517:1541	weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'	1517:1638	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	0	31	from	synbiotics	27:36	arg1	era					95:97	the microbiome era	80:97	the microbiome era	80:97	Probiotics, prebiotics and synbiotics for weight loss and metabolic syndrome in the microbiome era.					
30468509	8	32	theme	independent	1169:1179	arg1	effects					1208:1214	independent and synergistic beneficial effects	1169:1214	independent and synergistic beneficial effects	1169:1214	Fibers such as inulin or galactomannan promote independent and synergistic beneficial effects.					
30468509	3	33	theme	microbiome	345:354	arg1	composition					356:366	gut microbiome composition	341:366	gut microbiome composition	341:366	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	10	34	theme	inulin	1489:1494	arg1	fibers					1496:1501	inulin fibers	1489:1501	inulin fibers	1489:1501	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	9	35	theme	anti-inflammatory	1375:1391	arg1	activity					1393:1400	weight-reduction and anti-inflammatory activity	1354:1400	weight-reduction and anti-inflammatory activity	1354:1400	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	9	36	theme	independent	1411:1421	arg1	studies					1423:1429	large independent studies	1405:1429	large independent studies	1405:1429	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	6	37	theme	Several	875:881	arg1	studies					883:889	Several studies	875:889	RESULTS Several studies in humans and in animal models	867:920	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	3	38	theme	body	481:484	arg1	weight					486:491	excessive body weight	471:491	excessive body weight treatment	471:501	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	9	39	theme	gasseri	1321:1327	arg1	strains					1329:1335	Lactobacillus gasseri strains	1307:1335	Lactobacillus gasseri strains	1307:1335	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	3	40	dep	trials	324:329	arg1	functions					371:379	functions	371:379	functions with pro or prebiotics to promote a healthier profile	371:433	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	3	40	dep	trials	324:329	arg1	targeting					331:339	targeting	331:339	targeting gut microbiome composition	331:366	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	6	41	theme	clinical	984:991	arg1	efficacy					993:1000	the observed clinical efficacy	971:1000	the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management	971:1068	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	2	42	theme	conditions	303:312	arg1	cause					288:292	an important environmental cause	261:292	an important environmental cause of these conditions	261:312	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	2	42	theme	conditions	303:312	arg1	composition					246:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	6	43	theme	compounds	1038:1046	arg1	efficacy					993:1000	the observed clinical efficacy	971:1000	the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management	971:1068	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	3	44	theme	weight	486:491	arg1	treatment					493:501	excessive body weight treatment	471:501	excessive body weight treatment	471:501	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	4	45	theme	Cochrane	594:601	arg1	Library					603:609	Cochrane Library	594:609	Cochrane Library	594:609	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	5	46	theme	molecular	797:805	arg1	mechanisms					807:816	molecular mechanisms	797:816	molecular mechanisms supporting clinical results	797:844	Clinical studies and significant pre-clinical results showing molecular mechanisms supporting clinical results were also discussed.					
30468509	6	47	theme	observed	975:982	arg1	efficacy					993:1000	the observed clinical efficacy	971:1000	the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management	971:1068	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	2	48	theme	microbiota	235:244	arg1	cause					288:292	an important environmental cause	261:292	an important environmental cause of these conditions	261:312	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	2	48	theme	microbiota	235:244	arg1	composition					246:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	9	49	theme	selected	1281:1288	arg1	strains					1290:1296	selected strains	1281:1296	selected strains (such as Lactobacillus gasseri strains)	1281:1336	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	4	50	dep	MATERIALS	549:557	arg1	searched					574:581	searched	574:581	searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms	574:732	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	2	51	theme	gut	231:233	arg1	cause					288:292	an important environmental cause	261:292	an important environmental cause of these conditions	261:312	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	2	51	theme	gut	231:233	arg1	composition					246:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	10	52	theme	due	1543:1545	arg1	effects					1535:1541	weight management effects	1517:1541	weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'	1517:1638	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	3	53	theme	excessive	471:479	arg1	weight					486:491	excessive body weight	471:491	excessive body weight treatment	471:501	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	10	54	theme	microbiota	1610:1619	arg1	-configuration					1624:1637	microbiota 're-configuration	1610:1637	microbiota 're-configuration'	1610:1638	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	3	55	theme	Clinical	315:322	arg1	trials					324:329	Clinical trials	315:329	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile	315:433	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	6	56	theme	biological	938:947	arg1	mechanisms					949:958	biological mechanisms	938:958	biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management	938:1068	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	2	57	theme	altered	223:229	arg1	cause					288:292	an important environmental cause	261:292	an important environmental cause of these conditions	261:312	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	2	57	theme	altered	223:229	arg1	composition					246:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	1	58	theme	OBJECTIVE	100:108	arg1	fat					125:127	OBJECTIVE Excessive body fat	100:127	OBJECTIVE Excessive body fat	100:127	OBJECTIVE Excessive body fat and the associated dysmetabolic consequences affect both developed and emerging countries.					
30468509	3	59	theme	healthier	417:425	arg1	profile					427:433	a healthier profile	415:433	a healthier profile	415:433	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	10	60	theme	fatty	1584:1588	arg1	acid					1590:1593	short chain fatty acid	1572:1593	short chain fatty acid production	1572:1604	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	9	61	with	supplementation	1234:1248	arg1	synbiotics					1255:1264	synbiotics	1255:1264	synbiotics prepared using selected strains (such as Lactobacillus gasseri strains)	1255:1336	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	1	62	dep	affect	174:179	arg1	both					181:184	both	181:184	both	181:184	OBJECTIVE Excessive body fat and the associated dysmetabolic consequences affect both developed and emerging countries.					
30468509	1	62	dep	affect	174:179	arg1	developed					186:194	developed	186:194	affect both developed and emerging countries	174:217	OBJECTIVE Excessive body fat and the associated dysmetabolic consequences affect both developed and emerging countries.					
30468509	3	63	theme	complications	534:546	arg1	prevention					507:516	prevention	507:516	prevention of dysmetabolic complications	507:546	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	3	63	theme	complications	534:546	arg1	treatment					493:501	excessive body weight treatment	471:501	excessive body weight treatment	471:501	Clinical trials targeting gut microbiome composition or functions with pro or prebiotics to promote a healthier profile are considered a promising tool for excessive body weight treatment and prevention of dysmetabolic complications.					
30468509	1	64	theme	Excessive	110:118	arg1	fat					125:127	OBJECTIVE Excessive body fat	100:127	OBJECTIVE Excessive body fat	100:127	OBJECTIVE Excessive body fat and the associated dysmetabolic consequences affect both developed and emerging countries.					
30468509	6	65	theme	weight	1052:1057	arg1	management					1059:1068	weight management	1052:1068	weight management	1052:1068	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	5	66	theme	significant	756:766	arg1	results					781:787	significant pre-clinical results	756:787	significant pre-clinical results	756:787	Clinical studies and significant pre-clinical results showing molecular mechanisms supporting clinical results were also discussed.					
30468509	1	67	dep	countries	209:217	arg1	emerging					200:207	emerging	200:207	emerging	200:207	OBJECTIVE Excessive body fat and the associated dysmetabolic consequences affect both developed and emerging countries.					
30468509	2	68	theme	important	264:272	arg1	cause					288:292	an important environmental cause	261:292	an important environmental cause of these conditions	261:312	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	2	68	theme	important	264:272	arg1	composition					246:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition	220:256	An altered gut microbiota composition is an important environmental cause of these conditions.					
30468509	10	69	theme	weight	1517:1522	arg1	effects					1535:1541	weight management effects	1517:1541	weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'	1517:1638	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	1	70	theme	body	120:123	arg1	fat					125:127	OBJECTIVE Excessive body fat	100:127	OBJECTIVE Excessive body fat	100:127	OBJECTIVE Excessive body fat and the associated dysmetabolic consequences affect both developed and emerging countries.					
30468509	4	71	theme	synbiotics	659:668	arg1	terms					728:732	the search terms	717:732	the search terms	717:732	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	4	71	theme	synbiotics	659:668	arg1	combinations					617:628	combinations	617:628	combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome	617:712	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	5	72	theme	pre-clinical	768:779	arg1	results					781:787	significant pre-clinical results	756:787	significant pre-clinical results	756:787	Clinical studies and significant pre-clinical results showing molecular mechanisms supporting clinical results were also discussed.					
30468509	9	73	theme	Lactobacillus	1307:1319	arg1	strains					1329:1335	Lactobacillus gasseri strains	1307:1335	Lactobacillus gasseri strains	1307:1335	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	9	74	theme	weight-reduction	1354:1369	arg1	activity					1393:1400	weight-reduction and anti-inflammatory activity	1354:1400	weight-reduction and anti-inflammatory activity	1354:1400	CONCLUSIONS Diet supplementation with synbiotics prepared using selected strains (such as Lactobacillus gasseri strains) showed to exert weight-reduction and anti-inflammatory activity in large independent studies.					
30468509	0	75	theme	metabolic	58:66	arg1	syndrome					68:75	metabolic syndrome	58:75	metabolic syndrome	58:75	Probiotics, prebiotics and synbiotics for weight loss and metabolic syndrome in the microbiome era.					
30468509	10	76	from	effect	1562:1567	arg1	-configuration					1624:1637	microbiota 're-configuration	1610:1637	microbiota 're-configuration'	1610:1638	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	10	76	from	effect	1562:1567	arg1	production					1595:1604	short chain fatty acid production	1572:1604	short chain fatty acid production	1572:1604	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
30468509	4	77	with	combinations	617:628	arg1	syndrome					705:712	obesity/weight loss/metabolic syndrome	675:712	obesity/weight loss/metabolic syndrome	675:712	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	6	78	from	studies	883:889	arg1	humans					894:899	humans	894:899	humans	894:899	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	6	78	from	studies	883:889	arg1	models					915:920	animal models	908:920	animal models	908:920	RESULTS Several studies in humans and in animal models have elucidated biological mechanisms supporting the observed clinical efficacy of selected probiotic and prebiotic compounds for weight management.					
30468509	4	79	theme	obesity/weight	675:688	arg1	syndrome					705:712	obesity/weight loss/metabolic syndrome	675:712	obesity/weight loss/metabolic syndrome	675:712	MATERIALS AND METHODS We searched PubMed and Cochrane Library using combinations of probiotics/prebiotics and synbiotics with obesity/weight loss/metabolic syndrome as the search terms.					
30468509	10	80	theme	synergistic	1550:1560	arg1	effect					1562:1567	synergistic effect	1550:1567	synergistic effect on short chain fatty acid production and microbiota 're-configuration'	1550:1638	Their administration, together with galactomannan and/or inulin fibers, may increase weight management effects due to synergistic effect on short chain fatty acid production and microbiota 're-configuration'.					
31394143	6	0	with	paired	1090:1095	arg1	food					1102:1105	food	1102:1105	food	1102:1105	Rats were tested at 24 hrs, 7 d or 14 d whereby bar pressing resulted in presentation of cues paired with food but no food reward was delivered.					
31394143	10	1	theme	non-tested	1747:1756	arg1	values					1770:1775	the non-tested and control values	1743:1775	values	1770:1775	GluR1 AMPAr subunit levels were higher in the purified and sucrose groups tested at 24 hours compared to the non-tested and control values.					
31394143	13	2	from	rewards	2124:2130	arg1	abstinence					2093:2102	forced abstinence	2086:2102	forced abstinence from different food rewards	2086:2130	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	10	3	theme	subunit	1650:1656	arg1	levels					1658:1663	GluR1 AMPAr subunit levels	1638:1663	GluR1 AMPAr subunit levels	1638:1663	GluR1 AMPAr subunit levels were higher in the purified and sucrose groups tested at 24 hours compared to the non-tested and control values.					
31394143	11	4	theme	purified	1860:1867	arg1	groups					1903:1908	the purified and sucrose tested and non-tested groups	1856:1908	the purified and sucrose tested and non-tested groups	1856:1908	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	4	5	theme	different	713:721	arg1	types					728:732	different food types	713:732	different food types	713:732	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	4	6	theme	AMPA	861:864	arg1	levels					883:888	AMPA receptor protein levels	861:888	AMPA receptor protein levels	861:888	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	2	7	from	foods	336:340	arg1	abstinence					310:319	Prolonged forced abstinence	293:319	Prolonged forced abstinence from rewarding foods	293:340	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31394143	1	8	theme	Food	177:180	arg1	craving					182:188	Food craving	177:188	Food craving	177:188	Food craving can be viewed as an intense desire for a specific food that propagates seeking and consuming behavior.					
31394143	1	8	theme	Food	177:180	arg1	desire					218:223	an intense desire	207:223	an intense desire for a specific food that propagates seeking and consuming behavior	207:290	Food craving can be viewed as an intense desire for a specific food that propagates seeking and consuming behavior.					
31394143	9	9	theme	purified	1525:1532	arg1	group					1539:1543	The purified food group	1521:1543	The purified food group	1521:1543	The purified food group showed a time-dependent elevation in conditioned bar pressing over the 3 abstinence periods.					
31394143	13	10	theme	food	2202:2205	arg1	type					2207:2210	the food type	2198:2210	the food type	2198:2210	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	3	11	theme	receptors	595:603	arg1	composition					575:585	composition	575:585	composition	575:585	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	3	11	theme	receptors	595:603	arg1	abundance					561:569	abundance	561:569	abundance	561:569	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	6	12	theme	food	1114:1117	arg1	reward					1119:1124	no food reward	1111:1124	no food reward	1111:1124	Rats were tested at 24 hrs, 7 d or 14 d whereby bar pressing resulted in presentation of cues paired with food but no food reward was delivered.					
31394143	12	13	theme	pSer845	1948:1954	arg1	levels					1962:1967	GluR2 and pSer845 Glur1 levels	1938:1967	GluR2 and pSer845 Glur1 levels	1938:1967	GluR2 and pSer845 Glur1 levels were similar across all groups and abstinence periods.					
31394143	11	14	theme	sucrose	1873:1879	arg1	groups					1903:1908	the purified and sucrose tested and non-tested groups	1856:1908	the purified and sucrose tested and non-tested groups	1856:1908	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	8	15	dep	group	1283:1287	arg1	group					1468:1472	a group	1466:1472	1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control)	1278:1518	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	8	15	dep	group	1283:1287	arg1	control					1511:1517	control	1511:1517	control	1511:1517	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	8	15	dep	group	1283:1287	arg1	group					1385:1389	a group	1383:1389	1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control)	1278:1518	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	8	15	dep	group	1283:1287	arg1	1					1278:1278	1	1278:1278	1	1278:1278	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	11	16	theme	non-tested	1892:1901	arg1	groups					1903:1908	the purified and sucrose tested and non-tested groups	1856:1908	the purified and sucrose tested and non-tested groups	1856:1908	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	8	17	theme	tested	1365:1370	arg1	conditioned					1341:1351	conditioned	1341:1351	conditioned	1341:1351	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	8	17	theme	tested	1365:1370	arg1	group					1372:1376	tested group	1365:1376	tested group	1365:1376	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	14	18	from	levels	2280:2285	arg1	model					2295:2299	this model	2290:2299	this model	2290:2299	A clear link with AMPAr subunit levels in this model was not established.					
31394143	0	19	theme	conditioned	134:144	arg1	cues					146:149	conditioned cues	134:149	conditioned cues	134:149	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	4	20	from	changes	850:856	arg1	levels					883:888	AMPA receptor protein levels	861:888	AMPA receptor protein levels	861:888	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	13	21	theme	food	2183:2186	arg1	reward					2188:2193	the food reward	2179:2193	the food reward	2179:2193	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	11	22	theme	control	1922:1928	arg1	values					1930:1935	control values	1922:1935	control values	1922:1935	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	2	23	dep	Prolonged	293:301	arg1	forced					303:308	forced	303:308	forced	303:308	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31394143	10	24	theme	AMPAr	1644:1648	arg1	levels					1658:1663	GluR1 AMPAr subunit levels	1638:1663	GluR1 AMPAr subunit levels	1638:1663	GluR1 AMPAr subunit levels were higher in the purified and sucrose groups tested at 24 hours compared to the non-tested and control values.					
31394143	5	25	theme	bar	924:926	arg1	press					928:932	bar press	924:932	bar press for purified, sucrose, or chocolate-flavored sucrose pellets	924:993	Rats were trained for 10 days to bar press for purified, sucrose, or chocolate-flavored sucrose pellets.					
31394143	9	26	theme	conditioned	1582:1592	arg1	pressing					1598:1605	conditioned bar pressing	1582:1605	conditioned bar pressing	1582:1605	The purified food group showed a time-dependent elevation in conditioned bar pressing over the 3 abstinence periods.					
31394143	7	27	from	levels	1188:1193	arg1	NAc					1236:1238	the NAc	1232:1238	the NAc	1232:1238	Western blotting was used to determine protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc.					
31394143	12	28	theme	GluR2	1938:1942	arg1	levels					1962:1967	GluR2 and pSer845 Glur1 levels	1938:1967	GluR2 and pSer845 Glur1 levels	1938:1967	GluR2 and pSer845 Glur1 levels were similar across all groups and abstinence periods.					
31394143	0	29	theme	subunit	161:167	arg1	levels					169:174	AMPAr subunit levels	155:174	AMPAr subunit levels	155:174	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	8	30	dep	tested	1436:1441	arg1	group					1455:1459	non-tested group	1444:1459	non-tested group	1444:1459	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	11	31	theme	abstinence	1834:1843	arg1	periods					1845:1851	the 7- and 14-day abstinence periods	1816:1851	the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups	1816:1908	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	13	32	theme	macronutrient	2150:2162	arg1	composition					2164:2174	the macronutrient composition	2146:2174	the macronutrient composition of the food reward or the food type given during the abstinence period	2146:2245	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	11	33	theme	GluR1	1778:1782	arg1	levels					1784:1789	GluR1 levels	1778:1789	GluR1 levels	1778:1789	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	4	34	theme	present	682:688	arg1	study					690:694	The present study	678:694	The present study	678:694	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	7	35	theme	GluR1	1198:1202	arg1	levels					1188:1193	protein levels	1180:1193	protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc	1180:1238	Western blotting was used to determine protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc.					
31394143	11	36	dep	sucrose	1873:1879	arg1	tested					1881:1886	tested	1881:1886	tested	1881:1886	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	2	37	from	cues	440:443	arg1	absence					452:458	the absence	448:458	the absence of the primary reward	448:480	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31394143	3	38	from	changes	546:552	arg1	composition					575:585	composition	575:585	composition	575:585	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	3	38	from	changes	546:552	arg1	abundance					561:569	abundance	561:569	abundance	561:569	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	9	39	theme	abstinence	1618:1627	arg1	periods					1629:1635	the 3 abstinence periods	1612:1635	the 3 abstinence periods	1612:1635	The purified food group showed a time-dependent elevation in conditioned bar pressing over the 3 abstinence periods.					
31394143	0	40	theme	different	86:94	arg1	value					110:114	different macronutrient value	86:114	different macronutrient value	86:114	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	5	41	theme	chocolate-flavored	960:977	arg1	pellets					987:993	purified, sucrose, or chocolate-flavored sucrose pellets	938:993	pellets	987:993	Rats were trained for 10 days to bar press for purified, sucrose, or chocolate-flavored sucrose pellets.					
31394143	8	42	theme	separate	1247:1254	arg1	groups					1256:1261	Three separate groups	1241:1261	Three separate groups	1241:1261	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	14	43	with	link	2256:2259	arg1	levels					2280:2285	AMPAr subunit levels	2266:2285	AMPAr subunit levels in this model	2266:2299	A clear link with AMPAr subunit levels in this model was not established.					
31394143	4	44	theme	abstinence	794:803	arg1	periods					805:811	various abstinence periods	786:811	various abstinence periods	786:811	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	0	45	theme	value	110:114	arg1	reward					76:81	food reward	71:81	food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels	71:174	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	0	46	dep	different	86:94	arg1	macronutrient					96:108	macronutrient	96:108	macronutrient	96:108	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	0	47	theme	Different	0:8	arg1	periods					10:16	Different periods	0:16	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.	0:175	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	8	48	dep	group	1385:1389	arg1	2					1380:1380	2	1380:1380	2	1380:1380	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	4	49	theme	food-seeking	758:769	arg1	behavior					771:778	escalated food-seeking behavior	748:778	escalated food-seeking behavior	748:778	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	2	50	theme	food-paired	428:438	arg1	cues					440:443	food-paired cues	428:443	food-paired cues in the absence of the primary reward	428:480	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31394143	1	51	theme	consuming	273:281	arg1	behavior					283:290	consuming behavior	273:290	consuming behavior	273:290	Food craving can be viewed as an intense desire for a specific food that propagates seeking and consuming behavior.					
31394143	13	52	theme	different	2109:2117	arg1	rewards					2124:2130	different food rewards	2109:2130	different food rewards	2109:2130	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	2	53	theme	reward	475:480	arg1	absence					452:458	the absence	448:458	the absence of the primary reward	448:480	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31394143	0	54	theme	abstinence	28:37	arg1	periods					10:16	Different periods	0:16	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.	0:175	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	3	55	theme	food	493:496	arg1	consumption					498:508	Palatable food consumption	483:508	Palatable food consumption	483:508	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	0	56	theme	instrumental	45:56	arg1	learning					58:65	instrumental learning	45:65	instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels	45:174	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	14	57	theme	subunit	2272:2278	arg1	levels					2280:2285	AMPAr subunit levels	2266:2285	AMPAr subunit levels in this model	2266:2299	A clear link with AMPAr subunit levels in this model was not established.					
31394143	2	58	theme	rewarding	326:334	arg1	foods					336:340	rewarding foods	326:340	rewarding foods	326:340	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31394143	1	59	theme	intense	210:216	arg1	craving					182:188	Food craving	177:188	Food craving	177:188	Food craving can be viewed as an intense desire for a specific food that propagates seeking and consuming behavior.					
31394143	1	59	theme	intense	210:216	arg1	desire					218:223	an intense desire	207:223	an intense desire for a specific food that propagates seeking and consuming behavior	207:290	Food craving can be viewed as an intense desire for a specific food that propagates seeking and consuming behavior.					
31394143	6	60	theme	paired	1090:1095	arg1	cues					1085:1088	cues	1085:1088	cues paired with food	1085:1105	Rats were tested at 24 hrs, 7 d or 14 d whereby bar pressing resulted in presentation of cues paired with food but no food reward was delivered.					
31394143	2	61	theme	food-seeking	366:377	arg1	behavior					379:386	escalated food-seeking behavior	356:386	escalated food-seeking behavior	356:386	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31394143	11	62	from	periods	1845:1851	arg1	groups					1903:1908	the purified and sucrose tested and non-tested groups	1856:1908	the purified and sucrose tested and non-tested groups	1856:1908	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	13	63	theme	food	2119:2122	arg1	rewards					2124:2130	different food rewards	2109:2130	different food rewards	2109:2130	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	14	64	theme	clear	2250:2254	arg1	link					2256:2259	A clear link	2248:2259	A clear link with AMPAr subunit levels in this model	2248:2299	A clear link with AMPAr subunit levels in this model was not established.					
31394143	1	65	theme	specific	231:238	arg1	food					240:243	a specific food	229:243	a specific food that propagates seeking and consuming behavior	229:290	Food craving can be viewed as an intense desire for a specific food that propagates seeking and consuming behavior.					
31394143	4	66	theme	protein	875:881	arg1	levels					883:888	AMPA receptor protein levels	861:888	AMPA receptor protein levels	861:888	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	7	67	theme	GluR1pSer845	1205:1216	arg1	levels					1188:1193	protein levels	1180:1193	protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc	1180:1238	Western blotting was used to determine protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc.					
31394143	13	68	theme	reward	2188:2193	arg1	composition					2164:2174	the macronutrient composition	2146:2174	the macronutrient composition of the food reward or the food type given during the abstinence period	2146:2245	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	10	69	theme	GluR1	1638:1642	arg1	levels					1658:1663	GluR1 AMPAr subunit levels	1638:1663	GluR1 AMPAr subunit levels	1638:1663	GluR1 AMPAr subunit levels were higher in the purified and sucrose groups tested at 24 hours compared to the non-tested and control values.					
31394143	4	70	theme	receptor	866:873	arg1	levels					883:888	AMPA receptor protein levels	861:888	AMPA receptor protein levels	861:888	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	2	71	theme	Prolonged	293:301	arg1	abstinence					310:319	Prolonged forced abstinence	293:319	Prolonged forced abstinence from rewarding foods	293:340	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31394143	3	72	dep	nucleus	612:618	arg1	accumbens					620:628	accumbens	620:628	the nucleus accumbens (NAc)	608:634	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	3	72	dep	nucleus	612:618	arg1	NAc					631:633	NAc	631:633	NAc	631:633	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	9	73	theme	food	1534:1537	arg1	group					1539:1543	The purified food group	1521:1543	The purified food group	1521:1543	The purified food group showed a time-dependent elevation in conditioned bar pressing over the 3 abstinence periods.					
31394143	9	74	theme	time-dependent	1554:1567	arg1	elevation					1569:1577	a time-dependent elevation	1552:1577	a time-dependent elevation in conditioned bar pressing	1552:1605	The purified food group showed a time-dependent elevation in conditioned bar pressing over the 3 abstinence periods.					
31394143	12	75	theme	Glur1	1956:1960	arg1	levels					1962:1967	GluR2 and pSer845 Glur1 levels	1938:1967	GluR2 and pSer845 Glur1 levels	1938:1967	GluR2 and pSer845 Glur1 levels were similar across all groups and abstinence periods.					
31394143	11	76	theme	14-day	1827:1832	arg1	periods					1845:1851	the 7- and 14-day abstinence periods	1816:1851	the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups	1816:1908	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	0	77	theme	AMPAr	155:159	arg1	levels					169:174	AMPAr subunit levels	155:174	AMPAr subunit levels	155:174	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	3	78	theme	AMPA	590:593	arg1	receptors					595:603	AMPA receptors	590:603	AMPA receptors	590:603	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	3	79	from	abundance	561:569	arg1	nucleus					612:618	the nucleus accumbens (NAc)	608:634	the nucleus accumbens (NAc)	608:634	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	10	80	theme	purified	1684:1691	arg1	groups					1705:1710	the purified and sucrose groups	1680:1710	groups	1705:1710	GluR1 AMPAr subunit levels were higher in the purified and sucrose groups tested at 24 hours compared to the non-tested and control values.					
31394143	9	81	theme	bar	1594:1596	arg1	pressing					1598:1605	conditioned bar pressing	1582:1605	conditioned bar pressing	1582:1605	The purified food group showed a time-dependent elevation in conditioned bar pressing over the 3 abstinence periods.					
31394143	4	82	theme	food	723:726	arg1	types					728:732	different food types	713:732	different food types	713:732	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	3	83	from	composition	575:585	arg1	nucleus					612:618	the nucleus accumbens (NAc)	608:634	the nucleus accumbens (NAc)	608:634	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	0	84	theme	food	71:74	arg1	reward					76:81	food reward	71:81	food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels	71:174	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	11	85	theme	7-	1820:1821	arg1	periods					1845:1851	the 7- and 14-day abstinence periods	1816:1851	the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups	1816:1908	GluR1 levels subsequently declined at the 7- and 14-day abstinence periods in the purified and sucrose tested and non-tested groups compared to control values.					
31394143	3	86	dep	abundance	561:569	arg1	the					557:559	the	557:559	the	557:559	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	9	87	from	elevation	1569:1577	arg1	pressing					1598:1605	conditioned bar pressing	1582:1605	conditioned bar pressing	1582:1605	The purified food group showed a time-dependent elevation in conditioned bar pressing over the 3 abstinence periods.					
31394143	7	88	theme	protein	1180:1186	arg1	levels					1188:1193	protein levels	1180:1193	protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc	1180:1238	Western blotting was used to determine protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc.					
31394143	8	89	theme	operant	1415:1421	arg1	task					1423:1426	the operant task	1411:1426	the operant task	1411:1426	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	8	90	dep	group	1468:1472	arg1	3					1463:1463	3	1463:1463	3	1463:1463	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	7	91	used	used	1162:1165	arg2	blotting					1149:1156	Western blotting	1141:1156	Western blotting	1141:1156	Western blotting was used to determine protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc.					
31394143	5	92	theme	sucrose	979:985	arg1	pellets					987:993	purified, sucrose, or chocolate-flavored sucrose pellets	938:993	pellets	987:993	Rats were trained for 10 days to bar press for purified, sucrose, or chocolate-flavored sucrose pellets.					
31394143	4	93	theme	various	786:792	arg1	periods					805:811	various abstinence periods	786:811	various abstinence periods	786:811	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	7	94	theme	Western	1141:1147	arg1	blotting					1149:1156	Western blotting	1141:1156	Western blotting	1141:1156	Western blotting was used to determine protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc.					
31394143	5	95	theme	purified	938:945	arg1	sucrose					948:954	purified, sucrose, or chocolate-flavored sucrose pellets	938:993	sucrose	948:954	Rats were trained for 10 days to bar press for purified, sucrose, or chocolate-flavored sucrose pellets.					
31394143	6	96	theme	bar	1044:1046	arg1	pressing					1048:1055	bar pressing	1044:1055	bar pressing	1044:1055	Rats were tested at 24 hrs, 7 d or 14 d whereby bar pressing resulted in presentation of cues paired with food but no food reward was delivered.					
31394143	0	97	from	reward	76:81	arg1	responding					119:128	responding	119:128	responding	119:128	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	0	98	theme	forced	21:26	arg1	abstinence					28:37	forced abstinence	21:37	forced abstinence	21:37	Different periods of forced abstinence after instrumental learning for food reward of different macronutrient value on responding for conditioned cues and AMPAr subunit levels.					
31394143	13	99	theme	forced	2086:2091	arg1	abstinence					2093:2102	forced abstinence	2086:2102	forced abstinence from different food rewards	2086:2130	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	8	100	theme	non-tested	1444:1453	arg1	group					1455:1459	non-tested group	1444:1459	non-tested group	1444:1459	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	3	101	theme	differing	640:648	arg1	results					650:656	differing results	640:656	differing results	640:656	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	4	102	theme	escalated	748:756	arg1	behavior					771:778	escalated food-seeking behavior	748:778	escalated food-seeking behavior	748:778	The present study examined whether different food types could produce escalated food-seeking behavior after various abstinence periods and whether this was associated with changes in AMPA receptor protein levels.					
31394143	13	103	theme	abstinence	2229:2238	arg1	period					2240:2245	the abstinence period	2225:2245	the abstinence period	2225:2245	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	2	104	theme	primary	467:473	arg1	reward					475:480	the primary reward	463:480	the primary reward	463:480	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31394143	10	105	theme	sucrose	1697:1703	arg1	groups					1705:1710	the purified and sucrose groups	1680:1710	groups	1705:1710	GluR1 AMPAr subunit levels were higher in the purified and sucrose groups tested at 24 hours compared to the non-tested and control values.					
31394143	8	106	theme	operant	1313:1319	arg1	task					1321:1324	the operant task	1309:1324	the operant task	1309:1324	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	13	107	theme	food-seeking	2048:2059	arg1	behavior					2061:2068	food-seeking behavior	2048:2068	food-seeking behavior associated with forced abstinence from different food rewards	2048:2130	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	8	108	dep	assessed	1268:1275	arg1	group					1283:1287	a group	1281:1287	1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control)	1278:1518	Three separate groups were assessed: 1) a group that was trained on the operant task and tested for conditioned responding (tested group); 2) a group that was trained on the operant task but not tested (non-tested group); 3) a group that was neither trained nor tested (control).					
31394143	13	109	theme	type	2207:2210	arg1	composition					2164:2174	the macronutrient composition	2146:2174	the macronutrient composition of the food reward or the food type given during the abstinence period	2146:2245	These results show that food-seeking behavior associated with forced abstinence from different food rewards may depend on the macronutrient composition of the food reward or the food type given during the abstinence period.					
31394143	7	110	theme	GluR2	1223:1227	arg1	levels					1188:1193	protein levels	1180:1193	protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc	1180:1238	Western blotting was used to determine protein levels of GluR1, GluR1pSer845, and GluR2 in the NAc.					
31394143	14	111	theme	AMPAr	2266:2270	arg1	levels					2280:2285	AMPAr subunit levels	2266:2285	AMPAr subunit levels in this model	2266:2299	A clear link with AMPAr subunit levels in this model was not established.					
31394143	3	112	theme	Palatable	483:491	arg1	consumption					498:508	Palatable food consumption	483:508	Palatable food consumption	483:508	Palatable food consumption and food-seeking is associated with changes in the abundance and composition of AMPA receptors in the nucleus accumbens (NAc) but differing results have been reported.					
31394143	6	113	theme	cues	1085:1088	arg1	presentation					1069:1080	presentation	1069:1080	presentation of cues paired with food	1069:1105	Rats were tested at 24 hrs, 7 d or 14 d whereby bar pressing resulted in presentation of cues paired with food but no food reward was delivered.					
31394143	10	114	theme	control	1762:1768	arg1	values					1770:1775	the non-tested and control values	1743:1775	values	1770:1775	GluR1 AMPAr subunit levels were higher in the purified and sucrose groups tested at 24 hours compared to the non-tested and control values.					
31394143	12	115	theme	abstinence	2004:2013	arg1	periods					2015:2021	abstinence periods	2004:2021	abstinence periods	2004:2021	GluR2 and pSer845 Glur1 levels were similar across all groups and abstinence periods.					
31394143	2	116	theme	escalated	356:364	arg1	behavior					379:386	escalated food-seeking behavior	356:386	escalated food-seeking behavior	356:386	Prolonged forced abstinence from rewarding foods can result in escalated food-seeking behavior as measured via elevated responding for food-paired cues in the absence of the primary reward.					
31546409	2	0	theme	chitosan	533:540	arg1	layer					524:528	a thin layer	517:528	a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles	517:590	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	3	1	theme	fluorescence	626:637	arg1	XRF					653:655	XRF	653:655	XRF	653:655	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	1	theme	fluorescence	626:637	arg1	spectroscopy					639:650	X-ray fluorescence spectroscopy	620:650	X-ray fluorescence spectroscopy (XRF)	620:656	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	5	2	theme	biomedical	1100:1109	arg1	behavior					1111:1118	The biomedical behavior	1096:1118	The biomedical behavior of the fabricated PCL-based composite scaffold	1096:1165	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	4	3	theme	biocompatibility	961:976	arg1	tests					1047:1051	biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests	961:1051	biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold	961:1078	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	3	4	theme	X-ray	659:663	arg1	XRD					678:680	XRD	678:680	XRD	678:680	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	4	theme	X-ray	659:663	arg1	diffraction					665:675	X-ray diffraction	659:675	X-ray diffraction (XRD)	659:681	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	2	5	theme	Sr-substituted	560:573	arg1	nanoparticles					578:590	15 wt% Sr-substituted BG nanoparticles	553:590	15 wt% Sr-substituted BG nanoparticles	553:590	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	2	6	theme	15 wt	553:557	arg1	%					558:558	%	558:558	%	558:558	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	1	7	theme	solvent	412:418	arg1	technique					428:436	solvent casting technique	412:436	solvent casting technique for bone tissue engineering	412:464	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	6	8	theme	tissue	1443:1448	arg1	engineering					1450:1460	bone tissue engineering	1438:1460	bone tissue engineering	1438:1460	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	4	9	theme	alkaline	999:1006	arg1	activity					1018:1025	alkaline phosphate activity	999:1025	alkaline phosphate activity	999:1025	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	2	10	theme	selected	475:482	arg1	scaffold					492:499	a selected optimum scaffold	473:499	a selected optimum scaffold	473:499	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	1	11	theme	40 nm	354:358	arg1	size					340:343	a particle size	329:343	a particle size of about 40 nm	329:358	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	4	12	theme	scaffold	1071:1078	arg1	degradation					931:941	degradation	931:941	degradation	931:941	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	4	12	theme	scaffold	1071:1078	arg1	tests					1047:1051	biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests	961:1051	biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold	961:1078	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	4	12	theme	scaffold	1071:1078	arg1	bioactivity					944:954	bioactivity	944:954	bioactivity	944:954	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	3	13	theme	scanning	684:691	arg1	SEM					714:716	SEM	714:716	SEM	714:716	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	13	theme	scanning	684:691	arg1	microscopy					702:711	scanning electron microscopy	684:711	scanning electron microscopy (SEM)	684:717	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	14	theme	Fourier-transform	752:768	arg1	FTIR					793:796	FTIR	793:796	FTIR	793:796	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	14	theme	Fourier-transform	752:768	arg1	spectroscopy					779:790	Fourier-transform infrared spectroscopy	752:790	Fourier-transform infrared spectroscopy (FTIR)	752:797	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	6	15	used	utilized	1405:1412	arg2	candidate					1424:1432	a good candidate	1417:1432	a good candidate for bone tissue engineering	1417:1460	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	6	15	used	utilized	1405:1412	arg2	nanocomposite					1347:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite	1302:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles	1302:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	1	16	theme	%	376:376	arg1	Sr					389:390	Sr	389:390	Sr	389:390	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	16	theme	%	376:376	arg1	strontium					378:386	7 wt% strontium	372:386	7 wt% strontium (Sr)	372:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	17	theme	porous	167:172	arg1	scaffolds					204:212	porous (about 70 vol%) nanocomposite scaffolds	167:212	porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	167:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	18	theme	BG	305:306	arg1	nanoparticles					309:321	45S bioactive glass (BG) nanoparticles	284:321	45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	284:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	19	with	nanoparticles	309:321	arg1	size					340:343	a particle size	329:343	a particle size of about 40 nm	329:358	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	0	20	theme	bone	120:123	arg1	engineering					132:142	bone tissue engineering	120:142	bone tissue engineering	120:142	Fabrication of chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass nanocomposite scaffold for bone tissue engineering.					
31546409	1	21	theme	nanoparticles	309:321	arg1	nanoparticles					309:321	45S bioactive glass (BG) nanoparticles	284:321	45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	284:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	21	theme	nanoparticles	309:321	arg1	PCL					240:242	PCL	240:242	PCL	240:242	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	21	theme	nanoparticles	309:321	arg1	polycaprolactone					222:237	polycaprolactone	222:237	polycaprolactone (PCL)	222:243	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	21	theme	nanoparticles	309:321	arg1	%					278:278	0 to 15 wt%	268:278	0 to 15 wt%	268:278	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	21	theme	nanoparticles	309:321	arg1	amounts					259:265	different amounts	249:265	different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	249:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	22	theme	vol	184:186	arg1	%					187:187	about 70 vol%	175:187	about 70 vol%	175:187	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	23	dep	%	278:278	arg1	to					270:271	to	270:271	to	270:271	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	3	24	theme	light	728:732	arg1	scattering					734:743	dynamic light scattering	720:743	dynamic light scattering (DLS)	720:749	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	24	theme	light	728:732	arg1	DLS					746:748	DLS	746:748	DLS	746:748	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	2	25	theme	BG	575:576	arg1	nanoparticles					578:590	15 wt% Sr-substituted BG nanoparticles	553:590	15 wt% Sr-substituted BG nanoparticles	553:590	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	5	26	theme	nanoparticles	1246:1258	arg1	presence					1202:1209	the presence	1198:1209	the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating	1198:1284	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	1	27	theme	particle	331:338	arg1	size					340:343	a particle size	329:343	a particle size of about 40 nm	329:358	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	0	28	theme	bioactive	77:85	arg1	glass					87:91	chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass	15:91	chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass	15:91	Fabrication of chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass nanocomposite scaffold for bone tissue engineering.					
31546409	6	29	theme	15 wt	1372:1376	arg1	nanoparticles					1382:1394	15 wt% BG nanoparticles	1372:1394	15 wt% BG nanoparticles	1372:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	1	30	theme	15 wt	273:277	arg1	nanoparticles					309:321	45S bioactive glass (BG) nanoparticles	284:321	45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	284:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	30	theme	15 wt	273:277	arg1	%					278:278	0 to 15 wt%	268:278	0 to 15 wt%	268:278	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	30	theme	15 wt	273:277	arg1	amounts					259:265	different amounts	249:265	different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	249:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	3	31	theme	contact	824:830	arg1	measurement					838:848	water contact angle measurement	818:848	water contact angle measurement	818:848	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	5	32	theme	porosity	1218:1225	arg1	presence					1202:1209	the presence	1198:1209	the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating	1198:1284	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	0	33	theme	chitosan-coated	15:29	arg1	glass					87:91	chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass	15:91	chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass	15:91	Fabrication of chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass nanocomposite scaffold for bone tissue engineering.					
31546409	4	34	theme	In	900:901	arg1	degradation					931:941	degradation	931:941	degradation	931:941	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	4	34	theme	In	900:901	arg1	tests					1047:1051	biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests	961:1051	biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold	961:1078	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	4	34	theme	In	900:901	arg1	experiments					909:919	In vitro experiments	900:919	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold	900:1078	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	4	34	theme	In	900:901	arg1	bioactivity					944:954	bioactivity	944:954	bioactivity	944:954	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	3	35	theme	Several	593:599	arg1	techniques					601:610	Several techniques	593:610	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement	593:848	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	35	theme	Several	593:599	arg1	diffraction					665:675	X-ray diffraction	659:675	X-ray diffraction (XRD)	659:681	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	35	theme	Several	593:599	arg1	microscopy					702:711	scanning electron microscopy	684:711	scanning electron microscopy (SEM)	684:717	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	35	theme	Several	593:599	arg1	spectroscopy					639:650	X-ray fluorescence spectroscopy	620:650	X-ray fluorescence spectroscopy (XRF)	620:656	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	35	theme	Several	593:599	arg1	test					808:811	tensile test	800:811	tensile test	800:811	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	35	theme	Several	593:599	arg1	scattering					734:743	dynamic light scattering	720:743	dynamic light scattering (DLS)	720:749	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	35	theme	Several	593:599	arg1	measurement					838:848	water contact angle measurement	818:848	water contact angle measurement	818:848	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	35	theme	Several	593:599	arg1	spectroscopy					779:790	Fourier-transform infrared spectroscopy	752:790	Fourier-transform infrared spectroscopy (FTIR)	752:797	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	5	36	theme	Sr-substituted	1228:1241	arg1	nanoparticles					1246:1258	Sr-substituted BG nanoparticles	1228:1258	Sr-substituted BG nanoparticles	1228:1258	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	0	37	theme	polycaprolactone/strontium-substituted	38:75	arg1	glass					87:91	chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass	15:91	chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass	15:91	Fabrication of chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass nanocomposite scaffold for bone tissue engineering.					
31546409	1	38	theme	45S	284:286	arg1	nanoparticles					309:321	45S bioactive glass (BG) nanoparticles	284:321	45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	284:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	6	39	theme	porous	1333:1338	arg1	candidate					1424:1432	a good candidate	1417:1432	a good candidate for bone tissue engineering	1417:1460	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	6	39	theme	porous	1333:1338	arg1	nanocomposite					1347:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite	1302:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles	1302:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	2	40	contain	containing	542:551	arg1	chitosan					533:540	chitosan	533:540	chitosan containing 15 wt% Sr-substituted BG nanoparticles	533:590	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	2	40	contain	containing	542:551	arg2	nanoparticles					578:590	15 wt% Sr-substituted BG nanoparticles	553:590	15 wt% Sr-substituted BG nanoparticles	553:590	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	0	41	theme	glass	87:91	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass	0:91	Fabrication of chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass nanocomposite scaffold for bone tissue engineering.					
31546409	1	42	theme	glass	298:302	arg1	nanoparticles					309:321	45S bioactive glass (BG) nanoparticles	284:321	45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	284:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	3	43	theme	X-ray	620:624	arg1	XRF					653:655	XRF	653:655	XRF	653:655	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	43	theme	X-ray	620:624	arg1	spectroscopy					639:650	X-ray fluorescence spectroscopy	620:650	X-ray fluorescence spectroscopy (XRF)	620:656	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	1	44	theme	tissue	447:452	arg1	engineering					454:464	bone tissue engineering	442:464	bone tissue engineering	442:464	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	3	45	theme	fabricated	880:889	arg1	samples					891:897	the fabricated samples	876:897	the fabricated samples	876:897	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	5	46	theme	chitosan	1269:1276	arg1	coating					1278:1284	the chitosan coating	1265:1284	the chitosan coating	1265:1284	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	5	47	theme	PCL-based	1138:1146	arg1	scaffold					1158:1165	the fabricated PCL-based composite scaffold	1123:1165	the fabricated PCL-based composite scaffold	1123:1165	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	4	48	dep	In	900:901	arg1	vitro					903:907	vitro	903:907	vitro	903:907	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	6	49	theme	BG	1379:1380	arg1	nanoparticles					1382:1394	15 wt% BG nanoparticles	1372:1394	15 wt% BG nanoparticles	1372:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	2	50	theme	thin	519:522	arg1	layer					524:528	a thin layer	517:528	a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles	517:590	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	4	51	theme	cell	1032:1035	arg1	adhesion					1037:1044	cell adhesion	1032:1044	cell adhesion	1032:1044	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	6	52	theme	fabricated	1306:1315	arg1	candidate					1424:1432	a good candidate	1417:1432	a good candidate for bone tissue engineering	1417:1460	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	6	52	theme	fabricated	1306:1315	arg1	nanocomposite					1347:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite	1302:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles	1302:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	2	53	theme	%	558:558	arg1	nanoparticles					578:590	15 wt% Sr-substituted BG nanoparticles	553:590	15 wt% Sr-substituted BG nanoparticles	553:590	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	1	54	theme	nanocomposite	190:202	arg1	scaffolds					204:212	porous (about 70 vol%) nanocomposite scaffolds	167:212	porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	167:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	4	55	theme	phosphate	1008:1016	arg1	activity					1018:1025	alkaline phosphate activity	999:1025	alkaline phosphate activity	999:1025	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	1	56	theme	casting	420:426	arg1	technique					428:436	solvent casting technique	412:436	solvent casting technique for bone tissue engineering	412:464	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	6	57	theme	bone	1438:1441	arg1	engineering					1450:1460	bone tissue engineering	1438:1460	bone tissue engineering	1438:1460	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	2	58	theme	optimum	484:490	arg1	scaffold					492:499	a selected optimum scaffold	473:499	a selected optimum scaffold	473:499	Then, a selected optimum scaffold was coated with a thin layer of chitosan containing 15 wt% Sr-substituted BG nanoparticles.					
31546409	1	59	theme	present	152:158	arg1	study					160:164	the present study	148:164	the present study	148:164	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	3	60	theme	electron	693:700	arg1	SEM					714:716	SEM	714:716	SEM	714:716	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	60	theme	electron	693:700	arg1	microscopy					702:711	scanning electron microscopy	684:711	scanning electron microscopy (SEM)	684:717	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	1	61	theme	7 wt	372:375	arg1	Sr					389:390	Sr	389:390	Sr	389:390	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	61	theme	7 wt	372:375	arg1	strontium					378:386	7 wt% strontium	372:386	7 wt% strontium (Sr)	372:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	3	62	theme	infrared	770:777	arg1	FTIR					793:796	FTIR	793:796	FTIR	793:796	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	62	theme	infrared	770:777	arg1	spectroscopy					779:790	Fourier-transform infrared spectroscopy	752:790	Fourier-transform infrared spectroscopy (FTIR)	752:797	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	63	theme	dynamic	720:726	arg1	scattering					734:743	dynamic light scattering	720:743	dynamic light scattering (DLS)	720:749	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	63	theme	dynamic	720:726	arg1	DLS					746:748	DLS	746:748	DLS	746:748	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	4	64	theme	fabricated	1060:1069	arg1	scaffold					1071:1078	the fabricated scaffold	1056:1078	the fabricated scaffold	1056:1078	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	5	65	theme	scaffold	1158:1165	arg1	behavior					1111:1118	The biomedical behavior	1096:1118	The biomedical behavior of the fabricated PCL-based composite scaffold	1096:1165	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	0	66	theme	tissue	125:130	arg1	engineering					132:142	bone tissue engineering	120:142	bone tissue engineering	120:142	Fabrication of chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass nanocomposite scaffold for bone tissue engineering.					
31546409	1	67	dep	porous	167:172	arg1	%					187:187	about 70 vol%	175:187	about 70 vol%	175:187	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	5	68	theme	BG	1243:1244	arg1	nanoparticles					1246:1258	Sr-substituted BG nanoparticles	1228:1258	Sr-substituted BG nanoparticles	1228:1258	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	6	69	theme	%	1377:1377	arg1	nanoparticles					1382:1394	15 wt% BG nanoparticles	1372:1394	15 wt% BG nanoparticles	1372:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	4	70	dep	cytotoxicity	985:996	arg1	i.e.					979:982	i.e.	979:982	i.e.	979:982	In vitro experiments including degradation, bioactivity, and biocompatibility (i.e., cytotoxicity, alkaline phosphate activity, and cell adhesion) tests of the fabricated scaffold were performed.					
31546409	3	71	theme	tensile	800:806	arg1	test					808:811	tensile test	800:811	tensile test	800:811	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	0	72	theme	porous	31:36	arg1	glass					87:91	chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass	15:91	chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass	15:91	Fabrication of chitosan-coated porous polycaprolactone/strontium-substituted bioactive glass nanocomposite scaffold for bone tissue engineering.					
31546409	3	73	theme	angle	832:836	arg1	measurement					838:848	water contact angle measurement	818:848	water contact angle measurement	818:848	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	6	74	theme	PCL/BG	1340:1345	arg1	candidate					1424:1432	a good candidate	1417:1432	a good candidate for bone tissue engineering	1417:1460	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	6	74	theme	PCL/BG	1340:1345	arg1	nanocomposite					1347:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite	1302:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles	1302:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	3	75	theme	water	818:822	arg1	measurement					838:848	water contact angle measurement	818:848	water contact angle measurement	818:848	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	1	76	contain	containing	361:370	arg1	nanoparticles					309:321	45S bioactive glass (BG) nanoparticles	284:321	45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	284:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	76	contain	containing	361:370	arg2	strontium					378:386	7 wt% strontium	372:386	7 wt% strontium (Sr)	372:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	76	contain	containing	361:370	arg2	Sr					389:390	Sr	389:390	Sr	389:390	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	6	77	theme	chitosan-coated	1317:1331	arg1	candidate					1424:1432	a good candidate	1417:1432	a good candidate for bone tissue engineering	1417:1460	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	6	77	theme	chitosan-coated	1317:1331	arg1	nanocomposite					1347:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite	1302:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles	1302:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	1	78	theme	bioactive	288:296	arg1	nanoparticles					309:321	45S bioactive glass (BG) nanoparticles	284:321	45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	284:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	6	79	contain	containing	1361:1370	arg1	candidate					1424:1432	a good candidate	1417:1432	a good candidate for bone tissue engineering	1417:1460	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	6	79	contain	containing	1361:1370	arg2	nanoparticles					1382:1394	15 wt% BG nanoparticles	1372:1394	15 wt% BG nanoparticles	1372:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	6	79	contain	containing	1361:1370	arg1	nanocomposite					1347:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite	1302:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles	1302:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	1	80	theme	bone	442:445	arg1	engineering					454:464	bone tissue engineering	442:464	bone tissue engineering	442:464	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	6	81	theme	good	1419:1422	arg1	candidate					1424:1432	a good candidate	1417:1432	a good candidate for bone tissue engineering	1417:1460	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	6	81	theme	good	1419:1422	arg1	nanocomposite					1347:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite	1302:1359	the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles	1302:1394	In conclusion, the fabricated chitosan-coated porous PCL/BG nanocomposite containing 15 wt% BG nanoparticles could be utilized as a good candidate for bone tissue engineering.					
31546409	5	82	theme	fabricated	1127:1136	arg1	scaffold					1158:1165	the fabricated PCL-based composite scaffold	1123:1165	the fabricated PCL-based composite scaffold	1123:1165	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	3	83	used	used	855:858	arg2	techniques					601:610	Several techniques	593:610	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement	593:848	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	83	used	used	855:858	arg2	test					808:811	tensile test	800:811	tensile test	800:811	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	83	used	used	855:858	arg2	measurement					838:848	water contact angle measurement	818:848	water contact angle measurement	818:848	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	83	used	used	855:858	arg2	diffraction					665:675	X-ray diffraction	659:675	X-ray diffraction (XRD)	659:681	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	83	used	used	855:858	arg2	scattering					734:743	dynamic light scattering	720:743	dynamic light scattering (DLS)	720:749	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	83	used	used	855:858	arg2	microscopy					702:711	scanning electron microscopy	684:711	scanning electron microscopy (SEM)	684:717	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	83	used	used	855:858	arg2	spectroscopy					639:650	X-ray fluorescence spectroscopy	620:650	X-ray fluorescence spectroscopy (XRF)	620:656	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	3	83	used	used	855:858	arg2	spectroscopy					779:790	Fourier-transform infrared spectroscopy	752:790	Fourier-transform infrared spectroscopy (FTIR)	752:797	Several techniques such as X-ray fluorescence spectroscopy (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), tensile test, and water contact angle measurement were used to characterize the fabricated samples.					
31546409	1	84	theme	different	249:257	arg1	nanoparticles					309:321	45S bioactive glass (BG) nanoparticles	284:321	45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	284:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	84	theme	different	249:257	arg1	%					278:278	0 to 15 wt%	268:278	0 to 15 wt%	268:278	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	1	84	theme	different	249:257	arg1	amounts					259:265	different amounts	249:265	different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr)	249:391	In the present study, porous (about 70 vol%) nanocomposite scaffolds made of polycaprolactone (PCL) and different amounts (0 to 15 wt%) of 45S bioactive glass (BG) nanoparticles (with a particle size of about 40 nm) containing 7 wt% strontium (Sr) were fabricated by solvent casting technique for bone tissue engineering.					
31546409	5	85	theme	coating	1278:1284	arg1	presence					1202:1209	the presence	1198:1209	the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating	1198:1284	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
31546409	5	86	theme	composite	1148:1156	arg1	scaffold					1158:1165	the fabricated PCL-based composite scaffold	1123:1165	the fabricated PCL-based composite scaffold	1123:1165	The biomedical behavior of the fabricated PCL-based composite scaffold was interpreted by considering the presence of the porosity, Sr-substituted BG nanoparticles, and the chitosan coating.					
30391170	7	0	theme	whey	1186:1189	arg1	factor					1218:1223	an important factor	1205:1223	an important factor	1205:1223	These differences may have an effect upon the organoleptic qualities of spirits produced from whey; therefore, whey source may be an important factor when fermenting and distilling whey.					
30391170	7	0	theme	whey	1186:1189	arg1	source					1191:1196	whey source	1186:1196	whey source	1186:1196	These differences may have an effect upon the organoleptic qualities of spirits produced from whey; therefore, whey source may be an important factor when fermenting and distilling whey.					
30391170	6	1	theme	unique	973:978	arg1	compounds					998:1006	unique volatile aromatic compounds	973:1006	unique volatile aromatic compounds	973:1006	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	0	2	theme	acid	76:79	arg1	whey					81:84	acid whey	76:84	acid whey	76:84	Volatile aroma composition of distillates produced from fermented sweet and acid whey.					
30391170	5	3	theme	headspace	836:844	arg1	microextraction					858:872	headspace solid-phase microextraction	836:872	headspace solid-phase microextraction	836:872	Volatile aroma compounds were extracted using headspace solid-phase microextraction and identified via gas chromatography-mass spectrometry.					
30391170	3	4	theme	initial	502:508	arg1	fermentation					510:521	the initial fermentation	498:521	the initial fermentation	498:521	Fermentation and distillation of the 2 whey types progressed in a similar manner, using Kluyveromyces marxianus for the initial fermentation and a glass still fitted with a Vigreux column for the subsequent distillation.					
30391170	6	5	theme	whey	946:949	arg1	distillates					951:961	Acid and sweet whey distillates	931:961	distillates	951:961	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	5	6	theme	solid-phase	846:856	arg1	microextraction					858:872	headspace solid-phase microextraction	836:872	headspace solid-phase microextraction	836:872	Volatile aroma compounds were extracted using headspace solid-phase microextraction and identified via gas chromatography-mass spectrometry.					
30391170	6	7	theme	peak	1049:1052	arg1	areas					1054:1058	compound peak areas	1040:1058	compound peak areas	1040:1058	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	1	8	theme	potable	151:157	arg1	spirit					169:174	a potable distilled spirit	149:174	a potable distilled spirit	149:174	Lactose within whey can be fermented and distilled to produce a potable distilled spirit.					
30391170	2	9	from	differences	319:329	arg1	compounds					349:357	volatile aroma compounds	334:357	volatile aroma compounds	334:357	The aim of this study was to determine if acid and sweet whey types can be fermented and distilled using similar processes and to investigate differences in volatile aroma compounds for the 2 distillates.					
30391170	2	10	theme	whey	234:237	arg1	types					239:243	sweet whey types	228:243	sweet whey types	228:243	The aim of this study was to determine if acid and sweet whey types can be fermented and distilled using similar processes and to investigate differences in volatile aroma compounds for the 2 distillates.					
30391170	1	11	theme	distilled	159:167	arg1	spirit					169:174	a potable distilled spirit	149:174	a potable distilled spirit	149:174	Lactose within whey can be fermented and distilled to produce a potable distilled spirit.					
30391170	0	12	theme	aroma	9:13	arg1	composition					15:25	Volatile aroma composition	0:25	Volatile aroma composition of distillates	0:40	Volatile aroma composition of distillates produced from fermented sweet and acid whey.					
30391170	5	13	theme	aroma	799:803	arg1	compounds					805:813	Volatile aroma compounds	790:813	Volatile aroma compounds	790:813	Volatile aroma compounds were extracted using headspace solid-phase microextraction and identified via gas chromatography-mass spectrometry.					
30391170	0	14	theme	Volatile	0:7	arg1	composition					15:25	Volatile aroma composition	0:25	Volatile aroma composition of distillates	0:40	Volatile aroma composition of distillates produced from fermented sweet and acid whey.					
30391170	6	15	theme	sweet	940:944	arg1	distillates					951:961	Acid and sweet whey distillates	931:961	distillates	951:961	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	4	16	theme	whey	776:779	arg1	samples					781:787	whey samples	776:787	whey samples	776:787	Ethanol content of the wash (fermented whey) varied considerably following each fermentation and ranged from 1.2 and 2.0% (wt/wt) with no clear trend between acid and sweet whey samples.					
30391170	5	17	theme	gas	893:895	arg1	spectrometry					917:928	gas chromatography-mass spectrometry	893:928	gas chromatography-mass spectrometry	893:928	Volatile aroma compounds were extracted using headspace solid-phase microextraction and identified via gas chromatography-mass spectrometry.					
30391170	2	18	theme	aroma	343:347	arg1	compounds					349:357	volatile aroma compounds	334:357	volatile aroma compounds	334:357	The aim of this study was to determine if acid and sweet whey types can be fermented and distilled using similar processes and to investigate differences in volatile aroma compounds for the 2 distillates.					
30391170	6	19	from	differences	1025:1035	arg1	areas					1054:1058	compound peak areas	1040:1058	compound peak areas	1040:1058	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	5	20	theme	chromatography-mass	897:915	arg1	spectrometry					917:928	gas chromatography-mass spectrometry	893:928	gas chromatography-mass spectrometry	893:928	Volatile aroma compounds were extracted using headspace solid-phase microextraction and identified via gas chromatography-mass spectrometry.					
30391170	3	21	theme	types	426:430	arg1	distillation					399:410	distillation	399:410	distillation	399:410	Fermentation and distillation of the 2 whey types progressed in a similar manner, using Kluyveromyces marxianus for the initial fermentation and a glass still fitted with a Vigreux column for the subsequent distillation.					
30391170	3	21	theme	types	426:430	arg1	Fermentation					382:393	Fermentation	382:393	Fermentation	382:393	Fermentation and distillation of the 2 whey types progressed in a similar manner, using Kluyveromyces marxianus for the initial fermentation and a glass still fitted with a Vigreux column for the subsequent distillation.					
30391170	7	22	theme	spirits	1147:1153	arg1	qualities					1134:1142	the organoleptic qualities	1117:1142	the organoleptic qualities of spirits produced from whey	1117:1172	These differences may have an effect upon the organoleptic qualities of spirits produced from whey; therefore, whey source may be an important factor when fermenting and distilling whey.					
30391170	6	23	theme	Acid	931:934	arg1	distillates					951:961	Acid and sweet whey distillates	931:961	distillates	951:961	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	7	24	theme	important	1208:1216	arg1	factor					1218:1223	an important factor	1205:1223	an important factor	1205:1223	These differences may have an effect upon the organoleptic qualities of spirits produced from whey; therefore, whey source may be an important factor when fermenting and distilling whey.					
30391170	7	24	theme	important	1208:1216	arg1	source					1191:1196	whey source	1186:1196	whey source	1186:1196	These differences may have an effect upon the organoleptic qualities of spirits produced from whey; therefore, whey source may be an important factor when fermenting and distilling whey.					
30391170	7	25	contain	have	1097:1100	arg2	effect					1105:1110	an effect	1102:1110	an effect	1102:1110	These differences may have an effect upon the organoleptic qualities of spirits produced from whey; therefore, whey source may be an important factor when fermenting and distilling whey.					
30391170	7	25	contain	have	1097:1100	arg1	differences					1081:1091	These differences	1075:1091	These differences	1075:1091	These differences may have an effect upon the organoleptic qualities of spirits produced from whey; therefore, whey source may be an important factor when fermenting and distilling whey.					
30391170	7	26	theme	organoleptic	1121:1132	arg1	qualities					1134:1142	the organoleptic qualities	1117:1142	the organoleptic qualities of spirits produced from whey	1117:1172	These differences may have an effect upon the organoleptic qualities of spirits produced from whey; therefore, whey source may be an important factor when fermenting and distilling whey.					
30391170	4	27	theme	wash	626:629	arg1	content					611:617	Ethanol content	603:617	Ethanol content of the wash (fermented whey)	603:646	Ethanol content of the wash (fermented whey) varied considerably following each fermentation and ranged from 1.2 and 2.0% (wt/wt) with no clear trend between acid and sweet whey samples.					
30391170	6	28	theme	significant	1013:1023	arg1	differences					1025:1035	significant differences	1013:1035	significant differences in compound peak areas	1013:1058	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	0	29	theme	distillates	30:40	arg1	composition					15:25	Volatile aroma composition	0:25	Volatile aroma composition of distillates	0:40	Volatile aroma composition of distillates produced from fermented sweet and acid whey.					
30391170	3	30	theme	Vigreux	555:561	arg1	column					563:568	a Vigreux column	553:568	a Vigreux column for the subsequent distillation	553:600	Fermentation and distillation of the 2 whey types progressed in a similar manner, using Kluyveromyces marxianus for the initial fermentation and a glass still fitted with a Vigreux column for the subsequent distillation.					
30391170	4	31	theme	Ethanol	603:609	arg1	content					611:617	Ethanol content	603:617	Ethanol content of the wash (fermented whey)	603:646	Ethanol content of the wash (fermented whey) varied considerably following each fermentation and ranged from 1.2 and 2.0% (wt/wt) with no clear trend between acid and sweet whey samples.					
30391170	0	32	theme	fermented	56:64	arg1	sweet					66:70	fermented sweet	56:70	fermented sweet	56:70	Volatile aroma composition of distillates produced from fermented sweet and acid whey.					
30391170	3	33	theme	similar	448:454	arg1	manner					456:461	a similar manner	446:461	a similar manner	446:461	Fermentation and distillation of the 2 whey types progressed in a similar manner, using Kluyveromyces marxianus for the initial fermentation and a glass still fitted with a Vigreux column for the subsequent distillation.					
30391170	6	34	theme	compound	1040:1047	arg1	areas					1054:1058	compound peak areas	1040:1058	compound peak areas	1040:1058	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	2	35	theme	study	193:197	arg1	aim					181:183	The aim	177:183	The aim of this study	177:197	The aim of this study was to determine if acid and sweet whey types can be fermented and distilled using similar processes and to investigate differences in volatile aroma compounds for the 2 distillates.					
30391170	2	36	theme	sweet	228:232	arg1	types					239:243	sweet whey types	228:243	sweet whey types	228:243	The aim of this study was to determine if acid and sweet whey types can be fermented and distilled using similar processes and to investigate differences in volatile aroma compounds for the 2 distillates.					
30391170	4	37	theme	clear	741:745	arg1	trend					747:751	no clear trend	738:751	no clear trend between acid and sweet whey samples	738:787	Ethanol content of the wash (fermented whey) varied considerably following each fermentation and ranged from 1.2 and 2.0% (wt/wt) with no clear trend between acid and sweet whey samples.					
30391170	6	38	contain	contained	963:971	arg2	compounds					998:1006	unique volatile aromatic compounds	973:1006	unique volatile aromatic compounds	973:1006	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	6	38	contain	contained	963:971	arg1	distillates					951:961	Acid and sweet whey distillates	931:961	distillates	951:961	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	3	39	theme	subsequent	578:587	arg1	distillation					589:600	the subsequent distillation	574:600	the subsequent distillation	574:600	Fermentation and distillation of the 2 whey types progressed in a similar manner, using Kluyveromyces marxianus for the initial fermentation and a glass still fitted with a Vigreux column for the subsequent distillation.					
30391170	2	40	theme	similar	282:288	arg1	processes					290:298	similar processes	282:298	similar processes	282:298	The aim of this study was to determine if acid and sweet whey types can be fermented and distilled using similar processes and to investigate differences in volatile aroma compounds for the 2 distillates.					
30391170	3	41	theme	whey	421:424	arg1	types					426:430	the 2 whey types	415:430	the 2 whey types	415:430	Fermentation and distillation of the 2 whey types progressed in a similar manner, using Kluyveromyces marxianus for the initial fermentation and a glass still fitted with a Vigreux column for the subsequent distillation.					
30391170	4	42	dep	acid	761:764	arg1	samples					781:787	whey samples	776:787	whey samples	776:787	Ethanol content of the wash (fermented whey) varied considerably following each fermentation and ranged from 1.2 and 2.0% (wt/wt) with no clear trend between acid and sweet whey samples.					
30391170	2	43	theme	volatile	334:341	arg1	compounds					349:357	volatile aroma compounds	334:357	volatile aroma compounds	334:357	The aim of this study was to determine if acid and sweet whey types can be fermented and distilled using similar processes and to investigate differences in volatile aroma compounds for the 2 distillates.					
30391170	5	44	theme	Volatile	790:797	arg1	compounds					805:813	Volatile aroma compounds	790:813	Volatile aroma compounds	790:813	Volatile aroma compounds were extracted using headspace solid-phase microextraction and identified via gas chromatography-mass spectrometry.					
30391170	6	45	theme	aromatic	989:996	arg1	compounds					998:1006	unique volatile aromatic compounds	973:1006	unique volatile aromatic compounds	973:1006	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30391170	4	46	theme	fermented	632:640	arg1	wash					626:629	the wash	622:629	the wash (fermented whey)	622:646	Ethanol content of the wash (fermented whey) varied considerably following each fermentation and ranged from 1.2 and 2.0% (wt/wt) with no clear trend between acid and sweet whey samples.					
30391170	4	46	theme	fermented	632:640	arg1	whey					642:645	fermented whey	632:645	fermented whey	632:645	Ethanol content of the wash (fermented whey) varied considerably following each fermentation and ranged from 1.2 and 2.0% (wt/wt) with no clear trend between acid and sweet whey samples.					
30391170	4	47	dep	varied	648:653	arg1	following					668:676	following	668:676	varied considerably following each fermentation	648:694	Ethanol content of the wash (fermented whey) varied considerably following each fermentation and ranged from 1.2 and 2.0% (wt/wt) with no clear trend between acid and sweet whey samples.					
30391170	6	48	theme	volatile	980:987	arg1	compounds					998:1006	unique volatile aromatic compounds	973:1006	unique volatile aromatic compounds	973:1006	Acid and sweet whey distillates contained unique volatile aromatic compounds, and significant differences in compound peak areas were observed.					
30202908	4	0	theme	key	571:573	arg1	lineages					575:582	key lineages	571:582	key lineages of plants with stomata	571:605	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	9	1	with	congruent	1724:1732	arg1	change					1810:1815	environmental change	1796:1815	environmental change	1796:1815	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	1	with	congruent	1724:1732	arg1	inability					1743:1751	the inability	1739:1751	the inability of hornwort stomata to open	1739:1779	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	7	2	from	contrast	1275:1282	arg1	absent					1299:1304	absent	1299:1304	absent	1299:1304	In contrast, arabinans are absent in Phaeoceros, and both unesterified and methyl-esterified homogalacturonans localize throughout guard cell walls.					
30202908	6	3	theme	ledges	1217:1222	arg1	exterior					1191:1198	the exterior	1187:1198	the exterior of the wall, the ledges and the junction with adjacent epidermal cells	1187:1269	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	2	4	theme	cells	278:282	arg1	Walls					263:267	Walls	263:267	Walls of guard cells	263:282	Walls of guard cells are strong yet flexible because they open and close the pore by changing shape over the substomatal cavity.					
30202908	4	5	theme	diurnal	640:646	arg1	activity					657:664	diurnal stomatal activity	640:664	diurnal stomatal activity	640:664	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	5	6	theme	pectin	867:872	arg1	epitopes					883:890	pectin molecule epitopes	867:890	pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros	867:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	9	7	theme	hornwort	1756:1763	arg1	stomata					1765:1771	hornwort stomata	1756:1771	hornwort stomata	1756:1771	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	7	8	from	absent	1299:1304	arg1	contrast					1275:1282	contrast	1275:1282	contrast	1275:1282	In contrast, arabinans are absent in Phaeoceros, and both unesterified and methyl-esterified homogalacturonans localize throughout guard cell walls.					
30202908	7	8	from	absent	1299:1304	arg1	Phaeoceros					1309:1318	Phaeoceros	1309:1318	Phaeoceros	1309:1318	In contrast, arabinans are absent in Phaeoceros, and both unesterified and methyl-esterified homogalacturonans localize throughout guard cell walls.					
30202908	5	9	theme	epitopes	883:890	arg1	localization					851:862	the localization	847:862	the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros	847:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	6	10	with	wall	1207:1210	arg1	cells					1265:1269	adjacent epidermal cells	1246:1269	adjacent epidermal cells	1246:1269	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	7	11	from	Phaeoceros	1309:1318	arg1	absent					1299:1304	absent	1299:1304	absent	1299:1304	In contrast, arabinans are absent in Phaeoceros, and both unesterified and methyl-esterified homogalacturonans localize throughout guard cell walls.					
30202908	9	12	theme	high	1635:1638	arg1	levels					1640:1645	high levels	1635:1645	high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros	1635:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	1	13	theme	water	199:203	arg1	relations					205:213	water relations	199:213	water relations	199:213	BACKGROUND AND AIMS In seed plants, stomata regulate CO2 acquisition and water relations via transpiration, while minimizing water loss.					
30202908	10	14	theme	cell	1881:1884	arg1	composition					1891:1901	guard cell wall composition	1875:1901	guard cell wall composition	1875:1901	Comparisons across groups demonstrate that variations in guard cell wall composition reflect different physiological activity of stomata in land plants.					
30202908	6	15	theme	epidermal	1255:1263	arg1	cells					1265:1269	adjacent epidermal cells	1246:1269	adjacent epidermal cells	1246:1269	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	4	16	with	plants	587:592	arg1	stomata					599:605	stomata	599:605	stomata	599:605	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	5	17	theme	guard	925:929	arg1	walls					936:940	guard cell walls	925:940	guard cell walls of Arabidopsis and Phaeoceros	925:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	9	18	from	levels	1640:1645	arg1	walls					1700:1704	guard cell walls	1689:1704	guard cell walls of Phaeoceros	1689:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	19	from	congruent	1724:1732	arg1	contrast					1599:1606	contrast	1599:1606	contrast	1599:1606	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	8	20	theme	active	1538:1543	arg1	regulation					1545:1554	active regulation	1538:1554	active regulation of pore opening in Arabidopsis stomata	1538:1593	CONCLUSIONS Arabinans and unesterified homogalacturonans are required for wall flexibility, which is consistent with active regulation of pore opening in Arabidopsis stomata.					
30202908	7	21	theme	cell	1409:1412	arg1	walls					1414:1418	guard cell walls	1403:1418	guard cell walls	1403:1418	In contrast, arabinans are absent in Phaeoceros, and both unesterified and methyl-esterified homogalacturonans localize throughout guard cell walls.					
30202908	0	22	from	Phaeoceros	80:89	arg1	walls					42:46	guard cell walls	31:46	guard cell walls of Arabidopsis	31:61	Contrasting pectin polymers in guard cell walls of Arabidopsis and the hornwort Phaeoceros reflect physiological differences.					
30202908	0	23	theme	Arabidopsis	51:61	arg1	walls					42:46	guard cell walls	31:46	guard cell walls of Arabidopsis	31:61	Contrasting pectin polymers in guard cell walls of Arabidopsis and the hornwort Phaeoceros reflect physiological differences.					
30202908	10	24	theme	different	1911:1919	arg1	activity					1935:1942	different physiological activity	1911:1942	different physiological activity of stomata in land plants	1911:1968	Comparisons across groups demonstrate that variations in guard cell wall composition reflect different physiological activity of stomata in land plants.					
30202908	1	25	theme	seed	149:152	arg1	plants					154:159	seed plants	149:159	seed plants	149:159	BACKGROUND AND AIMS In seed plants, stomata regulate CO2 acquisition and water relations via transpiration, while minimizing water loss.					
30202908	10	26	from	variations	1861:1870	arg1	composition					1891:1901	guard cell wall composition	1875:1901	guard cell wall composition	1875:1901	Comparisons across groups demonstrate that variations in guard cell wall composition reflect different physiological activity of stomata in land plants.					
30202908	0	27	from	polymers	19:26	arg1	walls					42:46	guard cell walls	31:46	guard cell walls of Arabidopsis	31:61	Contrasting pectin polymers in guard cell walls of Arabidopsis and the hornwort Phaeoceros reflect physiological differences.					
30202908	6	28	theme	unesterified	1001:1012	arg1	homogalacturonans					1014:1030	Arabidopsis, unesterified homogalacturonans	988:1030	homogalacturonans	1014:1030	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	8	29	theme	Arabidopsis	1575:1585	arg1	stomata					1587:1593	Arabidopsis stomata	1575:1593	Arabidopsis stomata	1575:1593	CONCLUSIONS Arabinans and unesterified homogalacturonans are required for wall flexibility, which is consistent with active regulation of pore opening in Arabidopsis stomata.					
30202908	6	30	theme	Arabidopsis	988:998	arg1	homogalacturonans					1014:1030	Arabidopsis, unesterified homogalacturonans	988:1030	homogalacturonans	1014:1030	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	2	31	theme	substomatal	372:382	arg1	cavity					384:389	the substomatal cavity	368:389	the substomatal cavity	368:389	Walls of guard cells are strong yet flexible because they open and close the pore by changing shape over the substomatal cavity.					
30202908	0	32	theme	physiological	99:111	arg1	differences					113:123	physiological differences	99:123	physiological differences	99:123	Contrasting pectin polymers in guard cell walls of Arabidopsis and the hornwort Phaeoceros reflect physiological differences.					
30202908	9	33	theme	arabinans	1621:1629	arg1	levels					1640:1645	high levels	1635:1645	high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros	1635:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	33	theme	arabinans	1621:1629	arg1	lack					1613:1616	the lack	1609:1616	the lack of arabinans	1609:1629	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	33	theme	arabinans	1621:1629	arg1	congruent					1724:1732	congruent	1724:1732	congruent	1724:1732	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	4	34	theme	stomatal	713:720	arg1	movement					722:729	active stomatal movement	706:729	active stomatal movement	706:729	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	0	35	theme	Contrasting	0:10	arg1	polymers					19:26	Contrasting pectin polymers	0:26	Contrasting pectin polymers in guard cell walls of Arabidopsis	0:61	Contrasting pectin polymers in guard cell walls of Arabidopsis and the hornwort Phaeoceros reflect physiological differences.					
30202908	5	36	theme	Arabidopsis	945:955	arg1	walls					936:940	guard cell walls	925:940	guard cell walls of Arabidopsis and Phaeoceros	925:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	7	37	theme	methyl-esterified	1347:1363	arg1	homogalacturonans					1365:1381	both unesterified and methyl-esterified homogalacturonans	1325:1381	both unesterified and methyl-esterified homogalacturonans	1325:1381	In contrast, arabinans are absent in Phaeoceros, and both unesterified and methyl-esterified homogalacturonans localize throughout guard cell walls.					
30202908	5	38	theme	Phaeoceros	961:970	arg1	walls					936:940	guard cell walls	925:940	guard cell walls of Arabidopsis and Phaeoceros	925:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	7	39	theme	unesterified	1330:1341	arg1	homogalacturonans					1365:1381	both unesterified and methyl-esterified homogalacturonans	1325:1381	both unesterified and methyl-esterified homogalacturonans	1325:1381	In contrast, arabinans are absent in Phaeoceros, and both unesterified and methyl-esterified homogalacturonans localize throughout guard cell walls.					
30202908	4	40	theme	taxa	551:554	arg1	cells					538:542	guard cells	532:542	guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement	532:729	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	10	41	theme	land	1958:1961	arg1	plants					1963:1968	land plants	1958:1968	land plants	1958:1968	Comparisons across groups demonstrate that variations in guard cell wall composition reflect different physiological activity of stomata in land plants.					
30202908	0	42	theme	guard	31:35	arg1	walls					42:46	guard cell walls	31:46	guard cell walls of Arabidopsis	31:61	Contrasting pectin polymers in guard cell walls of Arabidopsis and the hornwort Phaeoceros reflect physiological differences.					
30202908	5	43	theme	transmission	779:790	arg1	microscopy					801:810	transmission electron microscopy	779:810	transmission electron microscopy	779:810	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	9	44	theme	homogalacturonans	1668:1684	arg1	levels					1640:1645	high levels	1635:1645	high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros	1635:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	44	theme	homogalacturonans	1668:1684	arg1	lack					1613:1616	the lack	1609:1616	the lack of arabinans	1609:1629	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	44	theme	homogalacturonans	1668:1684	arg1	congruent					1724:1732	congruent	1724:1732	congruent	1724:1732	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	6	45	theme	guard	1066:1070	arg1	walls					1077:1081	guard cell walls	1066:1081	guard cell walls	1066:1081	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	10	46	theme	stomata	1947:1953	arg1	activity					1935:1942	different physiological activity	1911:1942	different physiological activity of stomata in land plants	1911:1968	Comparisons across groups demonstrate that variations in guard cell wall composition reflect different physiological activity of stomata in land plants.					
30202908	9	47	theme	guard	1689:1693	arg1	walls					1700:1704	guard cell walls	1689:1704	guard cell walls of Phaeoceros	1689:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	4	48	theme	guard	532:536	arg1	cells					538:542	guard cells	532:542	guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement	532:729	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	8	49	theme	wall	1495:1498	arg1	flexibility					1500:1510	wall flexibility	1495:1510	wall flexibility	1495:1510	CONCLUSIONS Arabinans and unesterified homogalacturonans are required for wall flexibility, which is consistent with active regulation of pore opening in Arabidopsis stomata.					
30202908	4	50	theme	plants	587:592	arg1	lineages					575:582	key lineages	571:582	key lineages of plants with stomata	571:605	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	4	51	with	Arabidopsis	608:618	arg1	activity					657:664	diurnal stomatal activity	640:664	diurnal stomatal activity	640:664	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	9	52	theme	Phaeoceros	1709:1718	arg1	walls					1700:1704	guard cell walls	1689:1704	guard cell walls of Phaeoceros	1689:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	5	53	theme	immunolocalization	746:763	arg1	techniques					765:774	immunolocalization techniques	746:774	immunolocalization techniques	746:774	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	1	54	theme	water	251:255	arg1	loss					257:260	water loss	251:260	water loss	251:260	BACKGROUND AND AIMS In seed plants, stomata regulate CO2 acquisition and water relations via transpiration, while minimizing water loss.					
30202908	8	55	theme	opening	1564:1570	arg1	regulation					1545:1554	active regulation	1538:1554	active regulation of pore opening in Arabidopsis stomata	1538:1593	CONCLUSIONS Arabinans and unesterified homogalacturonans are required for wall flexibility, which is consistent with active regulation of pore opening in Arabidopsis stomata.					
30202908	9	56	from	contrast	1599:1606	arg1	congruent					1724:1732	congruent	1724:1732	congruent	1724:1732	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	56	from	contrast	1599:1606	arg1	lack					1613:1616	the lack	1609:1616	the lack of arabinans	1609:1629	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	10	57	from	activity	1935:1942	arg1	plants					1963:1968	land plants	1958:1968	land plants	1958:1968	Comparisons across groups demonstrate that variations in guard cell wall composition reflect different physiological activity of stomata in land plants.					
30202908	4	58	from	differences	495:505	arg1	composition					517:527	pectin composition	510:527	pectin composition	510:527	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	4	58	from	differences	495:505	arg1	cells					538:542	guard cells	532:542	guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement	532:729	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	4	59	with	Phaeoceros	671:680	arg1	activity					657:664	diurnal stomatal activity	640:664	diurnal stomatal activity	640:664	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	2	60	theme	guard	272:276	arg1	cells					278:282	guard cells	272:282	guard cells	272:282	Walls of guard cells are strong yet flexible because they open and close the pore by changing shape over the substomatal cavity.					
30202908	1	61	theme	CO2	179:181	arg1	acquisition					183:193	CO2 acquisition	179:193	CO2 acquisition	179:193	BACKGROUND AND AIMS In seed plants, stomata regulate CO2 acquisition and water relations via transpiration, while minimizing water loss.					
30202908	6	62	theme	wall	1207:1210	arg1	exterior					1191:1198	the exterior	1187:1198	the exterior of the wall, the ledges and the junction with adjacent epidermal cells	1187:1269	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	10	63	dep	different	1911:1919	arg1	physiological					1921:1933	physiological	1921:1933	physiological	1921:1933	Comparisons across groups demonstrate that variations in guard cell wall composition reflect different physiological activity of stomata in land plants.					
30202908	10	64	theme	guard	1875:1879	arg1	composition					1891:1901	guard cell wall composition	1875:1901	guard cell wall composition	1875:1901	Comparisons across groups demonstrate that variations in guard cell wall composition reflect different physiological activity of stomata in land plants.					
30202908	5	65	theme	molecule	874:881	arg1	epitopes					883:890	pectin molecule epitopes	867:890	pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros	867:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	9	66	theme	stomata	1765:1771	arg1	inability					1743:1751	the inability	1739:1751	the inability of hornwort stomata to open	1739:1779	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	4	67	theme	stomatal	648:655	arg1	activity					657:664	diurnal stomatal activity	640:664	diurnal stomatal activity	640:664	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	6	68	theme	KEY	973:975	arg1	RESULTS					977:983	KEY RESULTS	973:983	KEY RESULTS In Arabidopsis, unesterified homogalacturonans	973:1030	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	10	69	theme	wall	1886:1889	arg1	composition					1891:1901	guard cell wall composition	1875:1901	guard cell wall composition	1875:1901	Comparisons across groups demonstrate that variations in guard cell wall composition reflect different physiological activity of stomata in land plants.					
30202908	9	70	theme	environmental	1796:1808	arg1	change					1810:1815	environmental change	1796:1815	environmental change	1796:1815	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	6	71	theme	adjacent	1246:1253	arg1	cells					1265:1269	adjacent epidermal cells	1246:1269	adjacent epidermal cells	1246:1269	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	5	72	theme	cell	931:934	arg1	walls					936:940	guard cell walls	925:940	guard cell walls of Arabidopsis and Phaeoceros	925:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	8	73	theme	pore	1559:1562	arg1	opening					1564:1570	pore opening	1559:1570	pore opening	1559:1570	CONCLUSIONS Arabinans and unesterified homogalacturonans are required for wall flexibility, which is consistent with active regulation of pore opening in Arabidopsis stomata.					
30202908	6	74	theme	junction	1232:1239	arg1	exterior					1191:1198	the exterior	1187:1198	the exterior of the wall, the ledges and the junction with adjacent epidermal cells	1187:1269	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	5	75	theme	essential	892:900	arg1	epitopes					883:890	pectin molecule epitopes	867:890	pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros	867:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	7	76	theme	guard	1403:1407	arg1	walls					1414:1418	guard cell walls	1403:1418	guard cell walls	1403:1418	In contrast, arabinans are absent in Phaeoceros, and both unesterified and methyl-esterified homogalacturonans localize throughout guard cell walls.					
30202908	8	77	dep	CONCLUSIONS	1421:1431	arg1	Arabinans					1433:1441	Arabinans	1433:1441	Arabinans	1433:1441	CONCLUSIONS Arabinans and unesterified homogalacturonans are required for wall flexibility, which is consistent with active regulation of pore opening in Arabidopsis stomata.					
30202908	0	78	theme	hornwort	71:78	arg1	Phaeoceros					80:89	the hornwort Phaeoceros	67:89	the hornwort Phaeoceros	67:89	Contrasting pectin polymers in guard cell walls of Arabidopsis and the hornwort Phaeoceros reflect physiological differences.					
30202908	6	79	with	ledges	1217:1222	arg1	cells					1265:1269	adjacent epidermal cells	1246:1269	adjacent epidermal cells	1246:1269	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	5	80	theme	stomata	905:911	arg1	function					913:920	stomata function	905:920	stomata function in guard cell walls of Arabidopsis and Phaeoceros	905:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	4	81	theme	pectin	510:515	arg1	composition					517:527	pectin composition	510:527	pectin composition	510:527	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	5	82	from	function	913:920	arg1	walls					936:940	guard cell walls	925:940	guard cell walls of Arabidopsis and Phaeoceros	925:970	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	9	83	from	lack	1613:1616	arg1	walls					1700:1704	guard cell walls	1689:1704	guard cell walls of Phaeoceros	1689:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	8	84	from	regulation	1545:1554	arg1	stomata					1587:1593	Arabidopsis stomata	1575:1593	Arabidopsis stomata	1575:1593	CONCLUSIONS Arabinans and unesterified homogalacturonans are required for wall flexibility, which is consistent with active regulation of pore opening in Arabidopsis stomata.					
30202908	4	85	dep	taxa	551:554	arg1	Phaeoceros					671:680	Phaeoceros	671:680	Phaeoceros	671:680	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	4	85	dep	taxa	551:554	arg1	Arabidopsis					608:618	Arabidopsis	608:618	Arabidopsis	608:618	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	4	85	dep	taxa	551:554	arg1	angiosperm					624:633	an angiosperm	621:633	an angiosperm with diurnal stomatal activity	621:664	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	4	85	dep	taxa	551:554	arg1	taxa					551:554	two taxa	547:554	two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros	547:680	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	6	86	theme	arabinan	1109:1116	arg1	pectins					1118:1124	arabinan pectins	1109:1124	arabinan pectins	1109:1124	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	6	87	with	junction	1232:1239	arg1	cells					1265:1269	adjacent epidermal cells	1246:1269	adjacent epidermal cells	1246:1269	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	1	88	dep	BACKGROUND	126:135	arg1	regulate					170:177	regulate	170:177	regulate	170:177	BACKGROUND AND AIMS In seed plants, stomata regulate CO2 acquisition and water relations via transpiration, while minimizing water loss.					
30202908	0	89	theme	pectin	12:17	arg1	polymers					19:26	Contrasting pectin polymers	0:26	Contrasting pectin polymers in guard cell walls of Arabidopsis	0:61	Contrasting pectin polymers in guard cell walls of Arabidopsis and the hornwort Phaeoceros reflect physiological differences.					
30202908	9	90	from	walls	1700:1704	arg1	levels					1640:1645	high levels	1635:1645	high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros	1635:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	90	from	walls	1700:1704	arg1	lack					1613:1616	the lack	1609:1616	the lack of arabinans	1609:1629	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	90	from	walls	1700:1704	arg1	congruent					1724:1732	congruent	1724:1732	congruent	1724:1732	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	4	91	theme	active	706:711	arg1	movement					722:729	active stomatal movement	706:729	active stomatal movement	706:729	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	0	92	theme	cell	37:40	arg1	walls					42:46	guard cell walls	31:46	guard cell walls of Arabidopsis	31:61	Contrasting pectin polymers in guard cell walls of Arabidopsis and the hornwort Phaeoceros reflect physiological differences.					
30202908	9	93	theme	methyl-esterified	1650:1666	arg1	homogalacturonans					1668:1684	methyl-esterified homogalacturonans	1650:1684	methyl-esterified homogalacturonans in guard cell walls of Phaeoceros	1650:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	9	94	from	homogalacturonans	1668:1684	arg1	walls					1700:1704	guard cell walls	1689:1704	guard cell walls of Phaeoceros	1689:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	6	95	theme	cell	1072:1075	arg1	walls					1077:1081	guard cell walls	1066:1081	guard cell walls	1066:1081	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	3	96	theme	wall	418:421	arg1	flexibility					423:433	wall flexibility	418:433	wall flexibility	418:433	Pectins are necessary for wall flexibility and proper stomata functioning.					
30202908	6	97	from	RESULTS	977:983	arg1	homogalacturonans					1014:1030	Arabidopsis, unesterified homogalacturonans	988:1030	homogalacturonans	1014:1030	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
30202908	8	98	theme	unesterified	1447:1458	arg1	homogalacturonans					1460:1476	unesterified homogalacturonans	1447:1476	unesterified homogalacturonans	1447:1476	CONCLUSIONS Arabinans and unesterified homogalacturonans are required for wall flexibility, which is consistent with active regulation of pore opening in Arabidopsis stomata.					
30202908	3	99	theme	proper	439:444	arg1	stomata					446:452	proper stomata	439:452	proper stomata functioning	439:464	Pectins are necessary for wall flexibility and proper stomata functioning.					
30202908	5	100	theme	electron	792:799	arg1	microscopy					801:810	transmission electron microscopy	779:810	transmission electron microscopy	779:810	METHODS Using immunolocalization techniques in transmission electron microscopy, this study describes and compares the localization of pectin molecule epitopes essential to stomata function in guard cell walls of Arabidopsis and Phaeoceros.					
30202908	4	101	with	angiosperm	624:633	arg1	activity					657:664	diurnal stomatal activity	640:664	diurnal stomatal activity	640:664	This study investigates the differences in pectin composition in guard cells of two taxa that represent key lineages of plants with stomata: Arabidopsis, an angiosperm with diurnal stomatal activity, and Phaeoceros, a bryophyte that lacks active stomatal movement.					
30202908	8	102	with	consistent	1522:1531	arg1	regulation					1545:1554	active regulation	1538:1554	active regulation of pore opening in Arabidopsis stomata	1538:1593	CONCLUSIONS Arabinans and unesterified homogalacturonans are required for wall flexibility, which is consistent with active regulation of pore opening in Arabidopsis stomata.					
30202908	9	103	theme	cell	1695:1698	arg1	walls					1700:1704	guard cell walls	1689:1704	guard cell walls of Phaeoceros	1689:1718	In contrast, the lack of arabinans and high levels of methyl-esterified homogalacturonans in guard cell walls of Phaeoceros are congruent with the inability of hornwort stomata to open and close with environmental change.					
30202908	6	104	theme	methyl-esterified	1133:1149	arg1	homogalacturonans					1151:1167	methyl-esterified homogalacturonans	1133:1167	methyl-esterified homogalacturonans	1133:1167	KEY RESULTS In Arabidopsis, unesterified homogalacturonans very strongly localize throughout guard cell walls and are interspersed with arabinan pectins, while methyl-esterified homogalacturonans are restricted to the exterior of the wall, the ledges and the junction with adjacent epidermal cells.					
32255017	7	0	used	used	1447:1450	arg2	voltammetry					1396:1406	voltammetry	1396:1406	voltammetry	1396:1406	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	7	0	used	used	1447:1450	arg2	i-t					1430:1432	i-t	1430:1432	i-t	1430:1432	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	7	0	used	used	1447:1450	arg2	CV					1409:1410	CV	1409:1410	CV	1409:1410	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	1	1	theme	porous	291:296	arg1	NCs					324:326	CDs-3D-PG-Pd@Au NCs	308:326	CDs-3D-PG-Pd@Au NCs	308:326	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	1	1	theme	porous	291:296	arg1	graphene					298:305	three-dimensional porous graphene	273:305	three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs)	273:327	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	7	2	theme	electrocatalytic	1471:1486	arg1	reduction					1488:1496	the electrocatalytic reduction	1467:1496	the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th	1467:1558	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	9	3	theme	designed	1880:1887	arg1	method					1889:1894	the designed method	1876:1894	the designed method	1876:1894	Importantly, satisfactory results were obtained for analysing real serum samples, indicating that the designed method could provide an effective strategy in clinical research.					
32255017	8	4	theme	acceptable	1641:1650	arg1	stability					1652:1660	acceptable stability	1641:1660	acceptable stability	1641:1660	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	6	5	theme	electron	1278:1285	arg1	microscopy					1287:1296	scanning electron microscopy	1269:1296	scanning electron microscopy (SEM)	1269:1302	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	6	5	theme	electron	1278:1285	arg1	SEM					1299:1301	SEM	1299:1301	SEM	1299:1301	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	3	6	theme	large	567:571	arg1	NCs					590:592	Pd@Au NCs	584:592	Pd@Au NCs	584:592	Moreover, large amounts of Pd@Au NCs could load on the CDs-3D-PG, which effectively improved the electrochemical signals.					
32255017	3	6	theme	large	567:571	arg1	amounts					573:579	large amounts	567:579	large amounts of Pd@Au NCs	567:592	Moreover, large amounts of Pd@Au NCs could load on the CDs-3D-PG, which effectively improved the electrochemical signals.					
32255017	8	7	theme	troponin	1716:1723	arg1	cTnI					1728:1731	cardiac troponin I (cTnI)	1708:1732	cardiac troponin I (cTnI)	1708:1732	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	5	8	theme	carbon	869:874	arg1	AuNPs-FMCS-Th					885:897	AuNPs-FMCS-Th	885:897	AuNPs-FMCS-Th	885:897	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	5	8	theme	carbon	869:874	arg1	spheres					876:882	amino-functionalized microporous carbon spheres	836:882	amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th)	836:898	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	0	9	theme	Pd	184:185	arg1	nanocubes					190:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	3	10	theme	@	586:586	arg1	NCs					590:592	Pd@Au NCs	584:592	Pd@Au NCs	584:592	Moreover, large amounts of Pd@Au NCs could load on the CDs-3D-PG, which effectively improved the electrochemical signals.					
32255017	0	11	theme	functionalized	140:153	arg1	nanocubes					190:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	1	12	theme	Pd	216:217	arg1	nanocubes					222:230	Pd@Au nanocubes	216:230	Pd@Au nanocubes	216:230	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	6	13	theme	nanomaterials	1230:1242	arg1	composition					1199:1209	composition	1199:1209	composition	1199:1209	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	6	13	theme	nanomaterials	1230:1242	arg1	morphology					1184:1193	morphology	1184:1193	morphology	1184:1193	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	9	14	theme	clinical	1935:1942	arg1	research					1944:1951	clinical research	1935:1951	clinical research	1935:1951	Importantly, satisfactory results were obtained for analysing real serum samples, indicating that the designed method could provide an effective strategy in clinical research.					
32255017	0	15	theme	porous	158:163	arg1	nanocubes					190:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	5	16	theme	electrode	1071:1079	arg1	surface					1081:1087	the electrode surface	1067:1087	the electrode surface	1067:1087	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	9	17	theme	satisfactory	1791:1802	arg1	results					1804:1810	satisfactory results	1791:1810	satisfactory results	1791:1810	Importantly, satisfactory results were obtained for analysing real serum samples, indicating that the designed method could provide an effective strategy in clinical research.					
32255017	8	18	theme	detection	1745:1753	arg1	limit					1755:1759	a low detection limit	1739:1759	a low detection limit of 33.3 fg mL-1	1739:1775	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	1	19	theme	Au	219:220	arg1	nanocubes					222:230	Pd@Au nanocubes	216:230	Pd@Au nanocubes	216:230	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	5	20	theme	signal	1163:1168	arg1	response					1170:1177	the signal response	1159:1177	the signal response	1159:1177	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	6	21	dep	morphology	1184:1193	arg1	The					1180:1182	The	1180:1182	The	1180:1182	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	6	22	theme	UV-vis	1305:1310	arg1	spectroscopy					1312:1323	UV-vis spectroscopy	1305:1323	UV-vis spectroscopy	1305:1323	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	3	23	theme	NCs	590:592	arg1	NCs					590:592	Pd@Au NCs	584:592	Pd@Au NCs	584:592	Moreover, large amounts of Pd@Au NCs could load on the CDs-3D-PG, which effectively improved the electrochemical signals.					
32255017	3	23	theme	NCs	590:592	arg1	amounts					573:579	large amounts	567:579	large amounts of Pd@Au NCs	567:592	Moreover, large amounts of Pd@Au NCs could load on the CDs-3D-PG, which effectively improved the electrochemical signals.					
32255017	0	24	theme	Au	187:188	arg1	nanocubes					190:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	5	25	theme	transfer	1047:1054	arg1	process					1056:1062	the electron transfer process	1034:1062	the electron transfer process on the electrode surface	1034:1087	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	8	26	theme	fg	1769:1770	arg1	mL-1					1772:1775	33.3 fg mL-1	1764:1775	33.3 fg mL-1	1764:1775	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	2	27	theme	secondary	529:537	arg1	Ab2					551:553	Ab2	551:553	Ab2	551:553	β-cyclodextrins (CDs) were beneficial in improving the dispersibility of three-dimensional porous graphene (3D-PG) and displayed good capture capability towards secondary antibodies (Ab2).					
32255017	2	27	theme	secondary	529:537	arg1	antibodies					539:548	secondary antibodies	529:548	secondary antibodies (Ab2)	529:554	β-cyclodextrins (CDs) were beneficial in improving the dispersibility of three-dimensional porous graphene (3D-PG) and displayed good capture capability towards secondary antibodies (Ab2).					
32255017	4	28	theme	CDs-3D-PG-Pd	692:703	arg1	composite					712:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	4	28	theme	CDs-3D-PG-Pd	692:703	arg1	labels					759:764	signal amplification labels	738:764	signal amplification labels	738:764	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	5	29	from	process	1056:1062	arg1	surface					1081:1087	the electrode surface	1067:1087	the electrode surface	1067:1087	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	1	30	theme	Au	321:322	arg1	NCs					324:326	CDs-3D-PG-Pd@Au NCs	308:326	CDs-3D-PG-Pd@Au NCs	308:326	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	1	30	theme	Au	321:322	arg1	graphene					298:305	three-dimensional porous graphene	273:305	three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs)	273:327	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	4	31	theme	Au	705:706	arg1	composite					712:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	4	31	theme	Au	705:706	arg1	labels					759:764	signal amplification labels	738:764	signal amplification labels	738:764	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	0	32	theme	electrocatalytic	83:98	arg1	reduction					100:108	enhanced electrocatalytic reduction	74:108	enhanced electrocatalytic reduction of H2O2	74:116	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	5	33	theme	sensor	903:908	arg1	platforms					910:918	sensor platforms	903:918	sensor platforms	903:918	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	6	34	theme	transmission	1349:1360	arg1	microscopy					1371:1380	transmission electron microscopy	1349:1380	transmission electron microscopy (TEM)	1349:1386	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	6	34	theme	transmission	1349:1360	arg1	TEM					1383:1385	TEM	1383:1385	TEM	1383:1385	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	5	35	theme	mediated	1099:1106	arg1	effect					1108:1113	the mediated effect	1095:1113	the mediated effect of Th	1095:1119	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	4	36	theme	signal	738:743	arg1	composite					712:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	4	36	theme	signal	738:743	arg1	labels					759:764	signal amplification labels	738:764	signal amplification labels	738:764	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	0	37	theme	Ultrasensitive	0:13	arg1	immunosensor					29:40	Ultrasensitive sandwich-type immunosensor	0:40	Ultrasensitive sandwich-type immunosensor for cardiac troponin I	0:63	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	1	38	theme	CDs-3D-PG-Pd	308:319	arg1	NCs					324:326	CDs-3D-PG-Pd@Au NCs	308:326	CDs-3D-PG-Pd@Au NCs	308:326	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	1	38	theme	CDs-3D-PG-Pd	308:319	arg1	graphene					298:305	three-dimensional porous graphene	273:305	three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs)	273:327	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	2	39	theme	graphene	466:473	arg1	dispersibility					423:436	the dispersibility	419:436	the dispersibility of three-dimensional porous graphene (3D-PG)	419:481	β-cyclodextrins (CDs) were beneficial in improving the dispersibility of three-dimensional porous graphene (3D-PG) and displayed good capture capability towards secondary antibodies (Ab2).					
32255017	2	40	theme	three-dimensional	441:457	arg1	3D-PG					476:480	3D-PG	476:480	3D-PG	476:480	β-cyclodextrins (CDs) were beneficial in improving the dispersibility of three-dimensional porous graphene (3D-PG) and displayed good capture capability towards secondary antibodies (Ab2).					
32255017	2	40	theme	three-dimensional	441:457	arg1	graphene					466:473	three-dimensional porous graphene	441:473	three-dimensional porous graphene (3D-PG)	441:481	β-cyclodextrins (CDs) were beneficial in improving the dispersibility of three-dimensional porous graphene (3D-PG) and displayed good capture capability towards secondary antibodies (Ab2).					
32255017	5	41	theme	response	1170:1177	arg1	amplification					1142:1154	further amplification	1134:1154	further amplification of the signal response	1134:1177	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	5	42	theme	primary	960:966	arg1	Ab1					980:982	Ab1	980:982	Ab1	980:982	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	5	42	theme	primary	960:966	arg1	antibodies					968:977	primary antibodies	960:977	primary antibodies (Ab1)	960:983	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	2	43	theme	good	497:500	arg1	capability					510:519	good capture capability	497:519	good capture capability towards secondary antibodies (Ab2)	497:554	β-cyclodextrins (CDs) were beneficial in improving the dispersibility of three-dimensional porous graphene (3D-PG) and displayed good capture capability towards secondary antibodies (Ab2).					
32255017	3	44	from	load	600:603	arg1	CDs-3D-PG					612:620	the CDs-3D-PG	608:620	the CDs-3D-PG	608:620	Moreover, large amounts of Pd@Au NCs could load on the CDs-3D-PG, which effectively improved the electrochemical signals.					
32255017	0	45	theme	cardiac	46:52	arg1	troponin					54:61	cardiac troponin I	46:63	cardiac troponin I	46:63	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	7	46	theme	Au	1522:1523	arg1	NCs					1525:1527	CDs-3D-PG-Pd@Au NCs	1509:1527	CDs-3D-PG-Pd@Au NCs using electron mediation of Th	1509:1558	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	7	47	theme	CDs-3D-PG-Pd	1509:1520	arg1	NCs					1525:1527	CDs-3D-PG-Pd@Au NCs	1509:1527	CDs-3D-PG-Pd@Au NCs using electron mediation of Th	1509:1558	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	9	48	theme	real	1840:1843	arg1	samples					1851:1857	real serum samples	1840:1857	real serum samples	1840:1857	Importantly, satisfactory results were obtained for analysing real serum samples, indicating that the designed method could provide an effective strategy in clinical research.					
32255017	7	49	theme	H2O2	1501:1504	arg1	reduction					1488:1496	the electrocatalytic reduction	1467:1496	the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th	1467:1558	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	8	50	theme	good	1666:1669	arg1	reproducibility					1671:1685	good reproducibility	1666:1685	good reproducibility	1666:1685	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	1	51	theme	three-dimensional	273:289	arg1	NCs					324:326	CDs-3D-PG-Pd@Au NCs	308:326	CDs-3D-PG-Pd@Au NCs	308:326	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	1	51	theme	three-dimensional	273:289	arg1	graphene					298:305	three-dimensional porous graphene	273:305	three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs)	273:327	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	5	52	theme	Th	1118:1119	arg1	effect					1108:1113	the mediated effect	1095:1113	the mediated effect of Th	1095:1119	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	8	53	theme	high	1623:1626	arg1	selectivity					1628:1638	high selectivity	1623:1638	high selectivity	1623:1638	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	6	54	theme	scanning	1269:1276	arg1	microscopy					1287:1296	scanning electron microscopy	1269:1296	scanning electron microscopy (SEM)	1269:1302	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	6	54	theme	scanning	1269:1276	arg1	SEM					1299:1301	SEM	1299:1301	SEM	1299:1301	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	8	55	theme	cardiac	1708:1714	arg1	cTnI					1728:1731	cardiac troponin I (cTnI)	1708:1732	cardiac troponin I (cTnI)	1708:1732	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	5	56	theme	microporous	857:867	arg1	AuNPs-FMCS-Th					885:897	AuNPs-FMCS-Th	885:897	AuNPs-FMCS-Th	885:897	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	5	56	theme	microporous	857:867	arg1	spheres					876:882	amino-functionalized microporous carbon spheres	836:882	amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th)	836:898	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	5	57	theme	Au	780:781	arg1	AuNPs					798:802	AuNPs	798:802	AuNPs	798:802	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	5	57	theme	Au	780:781	arg1	nanoparticles					783:795	Au nanoparticles	780:795	Au nanoparticles (AuNPs)	780:803	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	3	58	theme	Pd	584:585	arg1	NCs					590:592	Pd@Au NCs	584:592	Pd@Au NCs	584:592	Moreover, large amounts of Pd@Au NCs could load on the CDs-3D-PG, which effectively improved the electrochemical signals.					
32255017	8	59	theme	I	1725:1725	arg1	cTnI					1728:1731	cardiac troponin I (cTnI)	1708:1732	cardiac troponin I (cTnI)	1708:1732	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	9	60	theme	effective	1913:1921	arg1	strategy					1923:1930	an effective strategy	1910:1930	an effective strategy in clinical research	1910:1951	Importantly, satisfactory results were obtained for analysing real serum samples, indicating that the designed method could provide an effective strategy in clinical research.					
32255017	0	61	theme	3D	155:156	arg1	nanocubes					190:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	8	62	theme	cTnI	1728:1731	arg1	detection					1695:1703	the detection	1691:1703	the detection of cardiac troponin I (cTnI)	1691:1732	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	6	63	theme	Raman	1326:1330	arg1	spectroscopy					1332:1343	Raman spectroscopy	1326:1343	Raman spectroscopy	1326:1343	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	1	64	theme	@	218:218	arg1	nanocubes					222:230	Pd@Au nanocubes	216:230	Pd@Au nanocubes	216:230	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	6	65	theme	as-prepared	1218:1228	arg1	nanomaterials					1230:1242	the as-prepared nanomaterials	1214:1242	the as-prepared nanomaterials	1214:1242	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	0	66	theme	@	186:186	arg1	nanocubes					190:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	3	67	theme	Au	587:588	arg1	NCs					590:592	Pd@Au NCs	584:592	Pd@Au NCs	584:592	Moreover, large amounts of Pd@Au NCs could load on the CDs-3D-PG, which effectively improved the electrochemical signals.					
32255017	8	68	theme	low	1741:1743	arg1	limit					1755:1759	a low detection limit	1739:1759	a low detection limit of 33.3 fg mL-1	1739:1775	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	9	69	from	strategy	1923:1930	arg1	research					1944:1951	clinical research	1935:1951	clinical research	1935:1951	Importantly, satisfactory results were obtained for analysing real serum samples, indicating that the designed method could provide an effective strategy in clinical research.					
32255017	4	70	theme	NCs	708:710	arg1	composite					712:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	4	70	theme	NCs	708:710	arg1	labels					759:764	signal amplification labels	738:764	signal amplification labels	738:764	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	5	71	theme	electron	1038:1045	arg1	process					1056:1062	the electron transfer process	1034:1062	the electron transfer process on the electrode surface	1034:1087	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	0	72	theme	enhanced	74:81	arg1	reduction					100:108	enhanced electrocatalytic reduction	74:108	enhanced electrocatalytic reduction of H2O2	74:116	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	7	73	dep	voltammetry	1396:1406	arg1	methods					1434:1440	methods	1434:1440	methods	1434:1440	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	4	74	theme	obtained	683:690	arg1	composite					712:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	4	74	theme	obtained	683:690	arg1	labels					759:764	signal amplification labels	738:764	signal amplification labels	738:764	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	1	75	theme	facile	353:358	arg1	method					360:365	a facile method	351:365	a facile method	351:365	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	8	76	theme	mL-1	1772:1775	arg1	limit					1755:1759	a low detection limit	1739:1759	a low detection limit of 33.3 fg mL-1	1739:1775	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	7	77	theme	Th	1557:1558	arg1	mediation					1544:1552	electron mediation	1535:1552	electron mediation of Th	1535:1558	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	2	78	theme	capture	502:508	arg1	capability					510:519	good capture capability	497:519	good capture capability towards secondary antibodies (Ab2)	497:554	β-cyclodextrins (CDs) were beneficial in improving the dispersibility of three-dimensional porous graphene (3D-PG) and displayed good capture capability towards secondary antibodies (Ab2).					
32255017	4	79	theme	@	704:704	arg1	composite					712:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	4	79	theme	@	704:704	arg1	labels					759:764	signal amplification labels	738:764	signal amplification labels	738:764	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	0	80	theme	H2O2	113:116	arg1	reduction					100:108	enhanced electrocatalytic reduction	74:108	enhanced electrocatalytic reduction of H2O2	74:116	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	4	81	used	utilized	726:733	arg2	composite					712:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	4	81	used	utilized	726:733	arg2	labels					759:764	signal amplification labels	738:764	signal amplification labels	738:764	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	6	82	theme	electron	1362:1369	arg1	microscopy					1371:1380	transmission electron microscopy	1349:1380	transmission electron microscopy (TEM)	1349:1386	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	6	82	theme	electron	1362:1369	arg1	TEM					1383:1385	TEM	1383:1385	TEM	1383:1385	The morphology and composition of the as-prepared nanomaterials were characterized using scanning electron microscopy (SEM), UV-vis spectroscopy, Raman spectroscopy and transmission electron microscopy (TEM).					
32255017	0	83	theme	β-cyclodextrins	124:138	arg1	nanocubes					190:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	0	84	theme	sandwich-type	15:27	arg1	immunosensor					29:40	Ultrasensitive sandwich-type immunosensor	0:40	Ultrasensitive sandwich-type immunosensor for cardiac troponin I	0:63	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	0	85	theme	graphene-supported	165:182	arg1	nanocubes					190:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes	124:198	Ultrasensitive sandwich-type immunosensor for cardiac troponin I based on enhanced electrocatalytic reduction of H2O2 using β-cyclodextrins functionalized 3D porous graphene-supported Pd@Au nanocubes.					
32255017	1	86	theme	@	320:320	arg1	NCs					324:326	CDs-3D-PG-Pd@Au NCs	308:326	CDs-3D-PG-Pd@Au NCs	308:326	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	1	86	theme	@	320:320	arg1	graphene					298:305	three-dimensional porous graphene	273:305	three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs)	273:327	In this study, Pd@Au nanocubes supported β-cyclodextrins functionalized three-dimensional porous graphene (CDs-3D-PG-Pd@Au NCs) was synthesized using a facile method.					
32255017	2	87	theme	porous	459:464	arg1	3D-PG					476:480	3D-PG	476:480	3D-PG	476:480	β-cyclodextrins (CDs) were beneficial in improving the dispersibility of three-dimensional porous graphene (3D-PG) and displayed good capture capability towards secondary antibodies (Ab2).					
32255017	2	87	theme	porous	459:464	arg1	graphene					466:473	three-dimensional porous graphene	441:473	three-dimensional porous graphene (3D-PG)	441:481	β-cyclodextrins (CDs) were beneficial in improving the dispersibility of three-dimensional porous graphene (3D-PG) and displayed good capture capability towards secondary antibodies (Ab2).					
32255017	4	88	theme	amplification	745:757	arg1	composite					712:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite	679:720	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	4	88	theme	amplification	745:757	arg1	labels					759:764	signal amplification labels	738:764	signal amplification labels	738:764	The obtained CDs-3D-PG-Pd@Au NCs composite was utilized as signal amplification labels.					
32255017	7	89	theme	electron	1535:1542	arg1	mediation					1544:1552	electron mediation	1535:1552	electron mediation of Th	1535:1558	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	8	90	theme	optimal	1567:1573	arg1	conditions					1575:1584	optimal conditions	1567:1584	optimal conditions	1567:1584	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	5	91	theme	further	1134:1140	arg1	amplification					1142:1154	further amplification	1134:1154	further amplification of the signal response	1134:1177	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	7	92	theme	@	1521:1521	arg1	NCs					1525:1527	CDs-3D-PG-Pd@Au NCs	1509:1527	CDs-3D-PG-Pd@Au NCs using electron mediation of Th	1509:1558	Cyclic voltammetry (CV) and amperometric i-t methods were used to investigate the electrocatalytic reduction of H2O2 by CDs-3D-PG-Pd@Au NCs using electron mediation of Th.					
32255017	9	93	theme	serum	1845:1849	arg1	samples					1851:1857	real serum samples	1840:1857	real serum samples	1840:1857	Importantly, satisfactory results were obtained for analysing real serum samples, indicating that the designed method could provide an effective strategy in clinical research.					
32255017	8	94	theme	proposed	1591:1598	arg1	immunosensor					1600:1611	the proposed immunosensor	1587:1611	the proposed immunosensor	1587:1611	Under optimal conditions, the proposed immunosensor exhibited high selectivity, acceptable stability and good reproducibility for the detection of cardiac troponin I (cTnI) with a low detection limit of 33.3 fg mL-1.					
32255017	3	95	theme	electrochemical	654:668	arg1	signals					670:676	the electrochemical signals	650:676	the electrochemical signals	650:676	Moreover, large amounts of Pd@Au NCs could load on the CDs-3D-PG, which effectively improved the electrochemical signals.					
32255017	5	96	theme	amino-functionalized	836:855	arg1	AuNPs-FMCS-Th					885:897	AuNPs-FMCS-Th	885:897	AuNPs-FMCS-Th	885:897	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
32255017	5	96	theme	amino-functionalized	836:855	arg1	spheres					876:882	amino-functionalized microporous carbon spheres	836:882	amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th)	836:898	Furthermore, Au nanoparticles (AuNPs) and thionine (Th) decorated on amino-functionalized microporous carbon spheres (AuNPs-FMCS-Th) as sensor platforms, which not only effectively immobilized primary antibodies (Ab1) by interacting with Au-NH2, but also accelerated the electron transfer process on the electrode surface using the mediated effect of Th, resulted in further amplification of the signal response.					
31146183	4	0	theme	residue	1243:1249	arg1	pericarp					1280:1287	the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp	1221:1287	the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d	1221:1300	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	5	1	theme	crystalline	1585:1595	arg1	formation					1597:1605	crystalline formation	1585:1605	crystalline formation	1585:1605	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	3	2	theme	firmness	973:980	arg1	increase					982:989	the firmness increase	969:989	the firmness increase	969:989	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	4	3	theme	firmness	1017:1024	arg1	concentrations					1161:1174	firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations	1017:1174	firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations	1017:1174	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	0	4	theme	green	183:187	arg1	stage					189:193	dark green stage	178:193	dark green stage	178:193	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	9	5	from	ethylene	2279:2286	arg1	olive					2291:2295	olive	2291:2295	olive	2291:2295	The experiments could be used for research on perception and transcription responses to ethylene in olive, a non-climacteric fruit.					
31146183	9	5	from	ethylene	2279:2286	arg1	fruit					2316:2320	a non-climacteric fruit	2298:2320	a non-climacteric fruit	2298:2320	The experiments could be used for research on perception and transcription responses to ethylene in olive, a non-climacteric fruit.					
31146183	2	6	theme	firmness	594:601	arg1	increases					603:611	fruit firmness increases	588:611	fruit firmness increases in concentration-dependent manner in a non-climacteric fruit	588:672	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	5	7	theme	DE	1653:1654	arg1	levels					1656:1661	DE levels	1653:1661	DE levels	1653:1661	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	7	theme	DE	1653:1654	arg1	CL					1581:1582	increased CL	1571:1582	increased CL (crystalline formation, as observed by microscopy)	1571:1633	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	10	8	from	practice	2326:2333	arg1	beneficial					2379:2388	beneficial	2379:2388	beneficial	2379:2388	In practice, high ethylene concentrations could also be beneficial for firmness increase and/or short storage of dark green olives.					
31146183	8	9	theme	first	2179:2183	arg1	time					2185:2188	first time	2179:2188	first time	2179:2188	Ethylene firming effect and the respective cell wall changes in olives are demonstrated for first time.					
31146183	4	10	theme	Konservolia	1261:1271	arg1	Konservolia					1261:1271	Konservolia	1261:1271	Konservolia	1261:1271	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	10	theme	Konservolia	1261:1271	arg1	AIR					1252:1254	AIR	1252:1254	AIR	1252:1254	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	10	theme	Konservolia	1261:1271	arg1	residue					1243:1249	the alcohol insoluble residue	1221:1249	the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d	1221:1300	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	5	11	from	pectins	1753:1759	arg1	day					1777:1779	day 10	1777:1782	day 10	1777:1782	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	11	from	pectins	1753:1759	arg1	fruit					1768:1772	all fruit	1764:1772	all fruit	1764:1772	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	10	12	from	beneficial	2379:2388	arg1	practice					2326:2333	practice	2326:2333	practice	2326:2333	In practice, high ethylene concentrations could also be beneficial for firmness increase and/or short storage of dark green olives.					
31146183	4	13	theme	fruit	1274:1278	arg1	pericarp					1280:1287	the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp	1221:1287	the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d	1221:1300	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	10	14	theme	ethylene	2341:2348	arg1	concentrations					2350:2363	high ethylene concentrations	2336:2363	high ethylene concentrations	2336:2363	In practice, high ethylene concentrations could also be beneficial for firmness increase and/or short storage of dark green olives.					
31146183	9	15	theme	perception	2237:2246	arg1	responses					2266:2274	perception and transcription responses	2237:2274	perception and transcription responses to ethylene in olive, a non-climacteric fruit	2237:2320	The experiments could be used for research on perception and transcription responses to ethylene in olive, a non-climacteric fruit.					
31146183	5	16	with	consistent	1555:1564	arg1	sugars					1642:1647	total sugars	1636:1647	total sugars	1636:1647	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	16	with	consistent	1555:1564	arg1	levels					1656:1661	DE levels	1653:1661	DE levels	1653:1661	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	16	with	consistent	1555:1564	arg1	CL					1581:1582	increased CL	1571:1582	increased CL (crystalline formation, as observed by microscopy)	1571:1633	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	4	17	theme	soluble	1092:1098	arg1	pectins					1100:1106	water soluble pectins	1086:1106	water soluble pectins (WSP)	1086:1112	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	17	theme	soluble	1092:1098	arg1	WSP					1109:1111	WSP	1109:1111	WSP	1109:1111	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	9	18	theme	transcription	2252:2264	arg1	responses					2266:2274	perception and transcription responses	2237:2274	perception and transcription responses to ethylene in olive, a non-climacteric fruit	2237:2320	The experiments could be used for research on perception and transcription responses to ethylene in olive, a non-climacteric fruit.					
31146183	10	19	theme	high	2336:2339	arg1	concentrations					2350:2363	high ethylene concentrations	2336:2363	high ethylene concentrations	2336:2363	In practice, high ethylene concentrations could also be beneficial for firmness increase and/or short storage of dark green olives.					
31146183	4	20	from	changes	1030:1036	arg1	pectins					1071:1077	total pectins	1065:1077	total pectins (TPC)	1065:1083	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	20	from	changes	1030:1036	arg1	pectins					1100:1106	water soluble pectins	1086:1106	water soluble pectins (WSP)	1086:1112	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	20	from	changes	1030:1036	arg1	cellulose					1049:1057	cellulose	1049:1057	cellulose (CL)	1049:1062	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	20	from	changes	1030:1036	arg1	sugars					1139:1144	total non-cellulosic sugars	1118:1144	total non-cellulosic sugars (total sugars)	1118:1159	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	20	from	changes	1030:1036	arg1	WSP					1109:1111	WSP	1109:1111	WSP	1109:1111	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	20	from	changes	1030:1036	arg1	CL					1060:1061	CL	1060:1061	CL	1060:1061	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	20	from	changes	1030:1036	arg1	TPC					1080:1082	TPC	1080:1082	TPC	1080:1082	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	20	from	changes	1030:1036	arg1	sugars					1153:1158	total sugars	1147:1158	total sugars	1147:1158	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	20	from	changes	1030:1036	arg1	lignin					1041:1046	lignin	1041:1046	lignin	1041:1046	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	2	21	theme	green	485:489	arg1	maturation					491:500	the green maturation	481:500	the green maturation	481:500	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	3	22	dep	perception	894:903	arg1	1-MCP					932:936	1-MCP	932:936	1-MCP	932:936	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	3	22	dep	perception	894:903	arg1	-methyl-cyclopropene					908:927	1 -methyl-cyclopropene	906:927	1 -methyl-cyclopropene	906:927	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	3	22	dep	perception	894:903	arg1	nitrate					950:956	silver nitrate	943:956	silver nitrate	943:956	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	4	23	from	firmness	1017:1024	arg1	pectins					1071:1077	total pectins	1065:1077	total pectins (TPC)	1065:1083	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	23	from	firmness	1017:1024	arg1	pectins					1100:1106	water soluble pectins	1086:1106	water soluble pectins (WSP)	1086:1112	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	23	from	firmness	1017:1024	arg1	cellulose					1049:1057	cellulose	1049:1057	cellulose (CL)	1049:1062	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	23	from	firmness	1017:1024	arg1	sugars					1139:1144	total non-cellulosic sugars	1118:1144	total non-cellulosic sugars (total sugars)	1118:1159	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	23	from	firmness	1017:1024	arg1	WSP					1109:1111	WSP	1109:1111	WSP	1109:1111	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	23	from	firmness	1017:1024	arg1	CL					1060:1061	CL	1060:1061	CL	1060:1061	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	23	from	firmness	1017:1024	arg1	TPC					1080:1082	TPC	1080:1082	TPC	1080:1082	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	23	from	firmness	1017:1024	arg1	sugars					1153:1158	total sugars	1147:1158	total sugars	1147:1158	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	23	from	firmness	1017:1024	arg1	lignin					1041:1046	lignin	1041:1046	lignin	1041:1046	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	6	24	theme	energy	1933:1938	arg1	demand					1940:1945	energy demand	1933:1945	energy demand	1933:1945	However, on day 5 ethylene-treated olives exhibited a transitional phase during softening, characterized by retention of high TPC concentration and energy demand, as indicated by elevated respiration rates.					
31146183	2	25	theme	concentration-dependent	616:638	arg1	manner					640:645	concentration-dependent manner	616:645	concentration-dependent manner	616:645	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	6	26	theme	concentration	1915:1927	arg1	retention					1893:1901	retention	1893:1901	retention of high TPC concentration and energy demand	1893:1945	However, on day 5 ethylene-treated olives exhibited a transitional phase during softening, characterized by retention of high TPC concentration and energy demand, as indicated by elevated respiration rates.					
31146183	9	27	used	used	2216:2219	arg2	experiments					2195:2205	The experiments	2191:2205	The experiments	2191:2205	The experiments could be used for research on perception and transcription responses to ethylene in olive, a non-climacteric fruit.					
31146183	4	28	theme	total	1147:1151	arg1	sugars					1139:1144	total non-cellulosic sugars	1118:1144	total non-cellulosic sugars (total sugars)	1118:1159	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	28	theme	total	1147:1151	arg1	sugars					1153:1158	total sugars	1147:1158	total sugars	1147:1158	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	6	29	theme	ethylene-treated	1803:1818	arg1	olives					1820:1825	ethylene-treated olives	1803:1825	ethylene-treated olives	1803:1825	However, on day 5 ethylene-treated olives exhibited a transitional phase during softening, characterized by retention of high TPC concentration and energy demand, as indicated by elevated respiration rates.					
31146183	4	30	from	Pectins	1363:1369	arg1	AIR					1374:1376	AIR	1374:1376	AIR	1374:1376	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	30	from	Pectins	1363:1369	arg1	ethylene					1344:1351	1000 μL L-1 ethylene	1332:1351	1000 μL L-1 ethylene at 20 °C. Pectins in AIR	1332:1376	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	31	theme	20 °C.	1356:1361	arg1	Pectins					1363:1369	20 °C. Pectins	1356:1369	20 °C. Pectins in AIR	1356:1376	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	0	32	theme	inhibitor	96:104	arg1	1-MCP					128:132	1-MCP	128:132	1-MCP	128:132	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	0	32	theme	inhibitor	96:104	arg1	1-methylcyclopropene					106:125	the ethylene inhibitor 1-methylcyclopropene	83:125	the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality	83:249	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	6	33	theme	high	1906:1909	arg1	concentration					1915:1927	high TPC concentration	1906:1927	high TPC concentration	1906:1927	However, on day 5 ethylene-treated olives exhibited a transitional phase during softening, characterized by retention of high TPC concentration and energy demand, as indicated by elevated respiration rates.					
31146183	2	34	theme	non-climacteric	652:666	arg1	fruit					668:672	a non-climacteric fruit	650:672	a non-climacteric fruit	650:672	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	10	35	theme	dark	2436:2439	arg1	olives					2447:2452	dark green olives	2436:2452	dark green olives	2436:2452	In practice, high ethylene concentrations could also be beneficial for firmness increase and/or short storage of dark green olives.					
31146183	0	36	theme	Increased	0:8	arg1	firmness					10:17	Increased firmness	0:17	Increased firmness	0:17	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	2	37	theme	dark	424:427	arg1	olives					449:454	dark green 'Konservolia' olives	424:454	dark green 'Konservolia' olives harvested shortly before the green maturation	424:500	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	4	38	from	degree	1206:1211	arg1	pericarp					1280:1287	the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp	1221:1287	the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d	1221:1300	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	39	theme	total	1118:1122	arg1	sugars					1139:1144	total non-cellulosic sugars	1118:1144	total non-cellulosic sugars (total sugars)	1118:1159	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	39	theme	total	1118:1122	arg1	sugars					1153:1158	total sugars	1147:1158	total sugars	1147:1158	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	2	40	dep	L-1	404:406	arg1	up					390:391	up	390:391	up	390:391	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	4	41	theme	1000 μL	1332:1338	arg1	ethylene					1344:1351	1000 μL L-1 ethylene	1332:1351	1000 μL L-1 ethylene at 20 °C. Pectins in AIR	1332:1376	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	8	42	theme	firming	2096:2102	arg1	effect					2104:2109	Ethylene firming effect	2087:2109	Ethylene firming effect	2087:2109	Ethylene firming effect and the respective cell wall changes in olives are demonstrated for first time.					
31146183	4	43	with	treatments	1316:1325	arg1	ethylene					1344:1351	1000 μL L-1 ethylene	1332:1351	1000 μL L-1 ethylene at 20 °C. Pectins in AIR	1332:1376	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	0	44	theme	cell	32:35	arg1	composition					42:52	modified cell wall composition	23:52	modified cell wall composition	23:52	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	6	45	theme	elevated	1964:1971	arg1	rates					1985:1989	elevated respiration rates	1964:1989	elevated respiration rates	1964:1989	However, on day 5 ethylene-treated olives exhibited a transitional phase during softening, characterized by retention of high TPC concentration and energy demand, as indicated by elevated respiration rates.					
31146183	4	46	theme	esterification	1191:1204	arg1	DE					1214:1215	DE	1214:1215	DE	1214:1215	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	46	theme	esterification	1191:1204	arg1	degree					1206:1211	pectin esterification degree	1184:1211	pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d	1184:1300	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	10	47	theme	olives	2447:2452	arg1	storage					2425:2431	short storage	2419:2431	short storage of dark green olives	2419:2452	In practice, high ethylene concentrations could also be beneficial for firmness increase and/or short storage of dark green olives.					
31146183	1	48	theme	ethylene	310:317	arg1	treatment					319:327	ethylene treatment	310:327	ethylene treatment	310:327	This study aimed to investigate the firmness retention by ethylene treatment in olive fruit, as observed earlier.					
31146183	8	49	theme	respective	2119:2128	arg1	changes					2140:2146	the respective cell wall changes	2115:2146	the respective cell wall changes in olives	2115:2156	Ethylene firming effect and the respective cell wall changes in olives are demonstrated for first time.					
31146183	9	50	from	research	2225:2232	arg1	responses					2266:2274	perception and transcription responses	2237:2274	perception and transcription responses to ethylene in olive, a non-climacteric fruit	2237:2320	The experiments could be used for research on perception and transcription responses to ethylene in olive, a non-climacteric fruit.					
31146183	8	51	theme	wall	2135:2138	arg1	changes					2140:2146	the respective cell wall changes	2115:2146	the respective cell wall changes in olives	2115:2156	Ethylene firming effect and the respective cell wall changes in olives are demonstrated for first time.					
31146183	3	52	theme	silver	943:948	arg1	nitrate					950:956	silver nitrate	943:956	silver nitrate	943:956	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	5	53	theme	increased	1532:1540	arg1	consistent					1555:1564	consistent	1555:1564	consistent	1555:1564	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	53	theme	increased	1532:1540	arg1	firmness					1542:1549	the increased firmness	1528:1549	the increased firmness	1528:1549	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	6	54	theme	transitional	1839:1850	arg1	phase					1852:1856	a transitional phase	1837:1856	a transitional phase	1837:1856	However, on day 5 ethylene-treated olives exhibited a transitional phase during softening, characterized by retention of high TPC concentration and energy demand, as indicated by elevated respiration rates.					
31146183	5	55	theme	increased	1571:1579	arg1	sugars					1642:1647	total sugars	1636:1647	total sugars	1636:1647	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	55	theme	increased	1571:1579	arg1	levels					1656:1661	DE levels	1653:1661	DE levels	1653:1661	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	55	theme	increased	1571:1579	arg1	CL					1581:1582	increased CL	1571:1582	increased CL (crystalline formation, as observed by microscopy)	1571:1633	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	2	56	dep	9	533:533	arg1	to					530:531	to	530:531	to	530:531	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	0	57	theme	dark	178:181	arg1	stage					189:193	dark green stage	178:193	dark green stage	178:193	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	5	58	theme	CDTA-soluble	1740:1751	arg1	pectins					1753:1759	CDTA-soluble pectins	1740:1759	CDTA-soluble pectins	1740:1759	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	4	59	theme	insoluble	1233:1241	arg1	Konservolia					1261:1271	Konservolia	1261:1271	Konservolia	1261:1271	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	59	theme	insoluble	1233:1241	arg1	AIR					1252:1254	AIR	1252:1254	AIR	1252:1254	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	59	theme	insoluble	1233:1241	arg1	residue					1243:1249	the alcohol insoluble residue	1221:1249	the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d	1221:1300	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	1	60	from	retention	297:305	arg1	fruit					338:342	olive fruit	332:342	olive fruit	332:342	This study aimed to investigate the firmness retention by ethylene treatment in olive fruit, as observed earlier.					
31146183	5	61	theme	total	1636:1640	arg1	sugars					1642:1647	total sugars	1636:1647	total sugars	1636:1647	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	61	theme	total	1636:1640	arg1	CL					1581:1582	increased CL	1571:1582	increased CL (crystalline formation, as observed by microscopy)	1571:1633	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	62	dep	observed	1611:1618	arg1	formation					1597:1605	crystalline formation	1585:1605	crystalline formation	1585:1605	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	4	63	theme	total	1065:1069	arg1	TPC					1080:1082	TPC	1080:1082	TPC	1080:1082	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	63	theme	total	1065:1069	arg1	pectins					1071:1077	total pectins	1065:1077	total pectins (TPC)	1065:1083	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	0	64	theme	Possible	197:204	arg1	implementation					206:219	Possible implementation	197:219	Possible implementation	197:219	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	4	65	theme	4 M	1483:1485	arg1	KOH					1487:1489	4 M KOH	1483:1489	4 M KOH	1483:1489	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	5	66	from	TPC	1732:1734	arg1	day					1777:1779	day 10	1777:1782	day 10	1777:1782	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	5	66	from	TPC	1732:1734	arg1	fruit					1768:1772	all fruit	1764:1772	all fruit	1764:1772	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	2	67	theme	fruit	588:592	arg1	increases					603:611	fruit firmness increases	588:611	fruit firmness increases in concentration-dependent manner in a non-climacteric fruit	588:672	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	8	68	from	changes	2140:2146	arg1	olives					2151:2156	olives	2151:2156	olives	2151:2156	Ethylene firming effect and the respective cell wall changes in olives are demonstrated for first time.					
31146183	5	69	dep	CL	1581:1582	arg1	observed					1611:1618	observed	1611:1618	observed by microscopy	1611:1632	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	0	70	theme	non-climacteric	142:156	arg1	olives					158:163	the non-climacteric olives	138:163	the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality	138:249	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	3	71	dep	5	804:804	arg1	to					801:802	to	801:802	to	801:802	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	0	72	from	1-methylcyclopropene	106:125	arg1	olives					158:163	the non-climacteric olives	138:163	the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality	138:249	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	2	73	from	increases	603:611	arg1	manner					640:645	concentration-dependent manner	616:645	concentration-dependent manner	616:645	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	2	73	from	increases	603:611	arg1	fruit					668:672	a non-climacteric fruit	650:672	a non-climacteric fruit	650:672	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	4	74	theme	5-d	1303:1305	arg1	treatments					1316:1325	5-d and 10-d treatments	1303:1325	5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR	1303:1376	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	75	theme	water	1086:1090	arg1	pectins					1100:1106	water soluble pectins	1086:1106	water soluble pectins (WSP)	1086:1112	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	75	theme	water	1086:1090	arg1	WSP					1109:1111	WSP	1109:1111	WSP	1109:1111	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	76	theme	10-d	1311:1314	arg1	treatments					1316:1325	5-d and 10-d treatments	1303:1325	5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR	1303:1376	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	1	77	theme	olive	332:336	arg1	fruit					338:342	olive fruit	332:342	olive fruit	332:342	This study aimed to investigate the firmness retention by ethylene treatment in olive fruit, as observed earlier.					
31146183	6	78	theme	demand	1940:1945	arg1	retention					1893:1901	retention	1893:1901	retention of high TPC concentration and energy demand	1893:1945	However, on day 5 ethylene-treated olives exhibited a transitional phase during softening, characterized by retention of high TPC concentration and energy demand, as indicated by elevated respiration rates.					
31146183	3	79	theme	ethoxyvinyl	862:872	arg1	synthesis					851:859	synthesis	851:859	synthesis (ethoxyvinyl glycine or AVG)	851:888	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	3	79	theme	ethoxyvinyl	862:872	arg1	glycine					874:880	ethoxyvinyl glycine or AVG	862:887	glycine	874:880	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	9	80	theme	non-climacteric	2300:2314	arg1	olive					2291:2295	olive	2291:2295	olive	2291:2295	The experiments could be used for research on perception and transcription responses to ethylene in olive, a non-climacteric fruit.					
31146183	9	80	theme	non-climacteric	2300:2314	arg1	fruit					2316:2320	a non-climacteric fruit	2298:2320	a non-climacteric fruit	2298:2320	The experiments could be used for research on perception and transcription responses to ethylene in olive, a non-climacteric fruit.					
31146183	3	81	theme	ethylene	813:820	arg1	inhibitors					822:831	all ethylene inhibitors	809:831	all ethylene inhibitors tested	809:838	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	0	82	theme	ethylene	87:94	arg1	1-MCP					128:132	1-MCP	128:132	1-MCP	128:132	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	0	82	theme	ethylene	87:94	arg1	1-methylcyclopropene					106:125	the ethylene inhibitor 1-methylcyclopropene	83:125	the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality	83:249	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	5	83	dep	showed	1504:1509	arg1	whereas					1681:1687	whereas	1681:1687	whereas	1681:1687	The results showed that on day 1.5, the increased firmness was consistent with increased CL (crystalline formation, as observed by microscopy), total sugars and DE levels, but reduced WSP, whereas softening reversed the changes and lowered TPC and CDTA-soluble pectins in all fruit on day 10.					
31146183	0	84	theme	ethylene	224:231	arg1	stage					189:193	dark green stage	178:193	dark green stage	178:193	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	6	85	theme	TPC	1911:1913	arg1	concentration					1915:1927	high TPC concentration	1906:1927	high TPC concentration	1906:1927	However, on day 5 ethylene-treated olives exhibited a transitional phase during softening, characterized by retention of high TPC concentration and energy demand, as indicated by elevated respiration rates.					
31146183	0	86	theme	olive	237:241	arg1	quality					243:249	olive quality	237:249	olive quality	237:249	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	7	87	theme	ethylene	2067:2074	arg1	treatment					2076:2084	ethylene treatment	2067:2084	ethylene treatment	2067:2084	The inhibitor 1-MCP, applied before ethylene, did inhibit the responses to ethylene treatment.					
31146183	2	88	theme	green	429:433	arg1	olives					449:454	dark green 'Konservolia' olives	424:454	dark green 'Konservolia' olives harvested shortly before the green maturation	424:500	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	4	89	theme	non-cellulosic	1124:1137	arg1	sugars					1139:1144	total non-cellulosic sugars	1118:1144	total non-cellulosic sugars (total sugars)	1118:1159	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	89	theme	non-cellulosic	1124:1137	arg1	sugars					1153:1158	total sugars	1147:1158	total sugars	1147:1158	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	2	90	theme	Ethylene	366:373	arg1	concentrations					375:388	Ethylene concentrations	366:388	Ethylene concentrations up to 1000 μL L-1	366:406	Ethylene concentrations up to 1000 μL L-1 were applied to dark green 'Konservolia' olives harvested shortly before the green maturation and exposed to 20 °C for up to 9 d. Surprisingly, the results indicated a tendency to fruit firmness increases in concentration-dependent manner in a non-climacteric fruit.					
31146183	0	91	theme	modified	23:30	arg1	composition					42:52	modified cell wall composition	23:52	modified cell wall composition	23:52	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	10	92	theme	firmness	2394:2401	arg1	increase					2403:2410	firmness increase	2394:2410	firmness increase	2394:2410	In practice, high ethylene concentrations could also be beneficial for firmness increase and/or short storage of dark green olives.					
31146183	4	93	theme	pectin	1184:1189	arg1	DE					1214:1215	DE	1214:1215	DE	1214:1215	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	93	theme	pectin	1184:1189	arg1	degree					1206:1211	pectin esterification degree	1184:1211	pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d	1184:1300	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	10	94	theme	short	2419:2423	arg1	storage					2425:2431	short storage	2419:2431	short storage of dark green olives	2419:2452	In practice, high ethylene concentrations could also be beneficial for firmness increase and/or short storage of dark green olives.					
31146183	0	95	theme	wall	37:40	arg1	composition					42:52	modified cell wall composition	23:52	modified cell wall composition	23:52	Increased firmness and modified cell wall composition by ethylene were reversed by the ethylene inhibitor 1-methylcyclopropene (1-MCP) in the non-climacteric olives harvested at dark green stage - Possible implementation of ethylene for olive quality.					
31146183	1	96	theme	firmness	288:295	arg1	retention					297:305	the firmness retention	284:305	the firmness retention by ethylene treatment in olive fruit	284:342	This study aimed to investigate the firmness retention by ethylene treatment in olive fruit, as observed earlier.					
31146183	6	97	theme	respiration	1973:1983	arg1	rates					1985:1989	elevated respiration rates	1964:1989	elevated respiration rates	1964:1989	However, on day 5 ethylene-treated olives exhibited a transitional phase during softening, characterized by retention of high TPC concentration and energy demand, as indicated by elevated respiration rates.					
31146183	8	98	theme	Ethylene	2087:2094	arg1	effect					2104:2109	Ethylene firming effect	2087:2109	Ethylene firming effect	2087:2109	Ethylene firming effect and the respective cell wall changes in olives are demonstrated for first time.					
31146183	4	99	theme	L-1	1340:1342	arg1	ethylene					1344:1351	1000 μL L-1 ethylene	1332:1351	1000 μL L-1 ethylene at 20 °C. Pectins in AIR	1332:1376	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	8	100	from	effect	2104:2109	arg1	olives					2151:2156	olives	2151:2156	olives	2151:2156	Ethylene firming effect and the respective cell wall changes in olives are demonstrated for first time.					
31146183	3	101	theme	highest	679:685	arg1	concentration					687:699	The highest concentration	675:699	The highest concentration	675:699	The highest concentration increased the firmness within 12 h by approximately 1.35-fold, but transiently for approximately up to 5 d; all ethylene inhibitors tested, either of synthesis (ethoxyvinyl glycine or AVG), or perception (1 -methyl-cyclopropene or 1-MCP, and silver nitrate) prevented the firmness increase.					
31146183	10	102	theme	green	2441:2445	arg1	olives					2447:2452	dark green olives	2436:2452	dark green olives	2436:2452	In practice, high ethylene concentrations could also be beneficial for firmness increase and/or short storage of dark green olives.					
31146183	4	103	theme	changes	1030:1036	arg1	concentrations					1161:1174	firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations	1017:1174	firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations	1017:1174	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	8	104	theme	cell	2130:2133	arg1	changes					2140:2146	the respective cell wall changes	2115:2146	the respective cell wall changes in olives	2115:2156	Ethylene firming effect and the respective cell wall changes in olives are demonstrated for first time.					
31146183	4	105	theme	alcohol	1225:1231	arg1	Konservolia					1261:1271	Konservolia	1261:1271	Konservolia	1261:1271	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	105	theme	alcohol	1225:1231	arg1	AIR					1252:1254	AIR	1252:1254	AIR	1252:1254	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	4	105	theme	alcohol	1225:1231	arg1	residue					1243:1249	the alcohol insoluble residue	1221:1249	the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d	1221:1300	Texture was evaluated by firmness and changes in lignin, cellulose (CL), total pectins (TPC), water soluble pectins (WSP) and total non-cellulosic sugars (total sugars) concentrations, and in pectin esterification degree (DE) in the alcohol insoluble residue (AIR) of 'Konservolia' fruit pericarp during 1.5-d, 5-d and 10-d treatments with 1000 μL L-1 ethylene at 20 °C. Pectins in AIR were also extracted sequentially with cyclohexane-trans-1,2-diaminetetra-acetate (CDTA), Na2CO3, 1 M and 4 M KOH.					
31146183	7	106	theme	inhibitor	1996:2004	arg1	1-MCP					2006:2010	The inhibitor 1-MCP	1992:2010	The inhibitor 1-MCP	1992:2010	The inhibitor 1-MCP, applied before ethylene, did inhibit the responses to ethylene treatment.					
30669251	3	0	theme	-with	689:693	arg1	potential					695:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential	582:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition	582:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	4	1	with	milk	922:925	arg1	mixture					942:948	prebiotic mixture	932:948	prebiotic mixture	932:948	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	3	2	theme	fermented	470:478	arg1	milk					480:483	a fermented milk	468:483	a fermented milk	468:483	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	8	3	from	components	1328:1337	arg1	milk					1356:1359	the fermented milk	1342:1359	the fermented milk in combination with the prebiotics used here	1342:1404	In conclusion, the postbiotic components in the fermented milk in combination with the prebiotics used here showed protective properties against RV infection.					
30669251	5	4	dep	such	1033:1036	arg1	as					1038:1039	as	1038:1039	as	1038:1039	The dietary intervention caused a reduction in the clinical symptoms of diarrhea, such as severity and incidence.					
30669251	0	5	theme	Fermented	64:72	arg1	Milk					74:77	a Specific Fermented Milk	53:77	a Specific Fermented Milk	53:77	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture.					
30669251	3	6	theme	scGOS/lcFOS	673:683	arg1	potential					695:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential	582:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition	582:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	3	7	theme	galactooligosaccharides/long	614:641	arg1	potential					695:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential	582:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition	582:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	6	8	theme	immune	1098:1103	arg1	response					1105:1112	the immune response	1094:1112	the immune response	1094:1112	Furthermore, a modulation of the immune response was observed, which might enhance the reduction of the associated diarrhea.					
30669251	0	9	from	Prevention	0:9	arg1	Rats					45:48	Suckling Rats	36:48	Suckling Rats	36:48	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture.					
30669251	7	10	with	milk	1217:1220	arg1	mixture					1237:1243	prebiotic mixture	1227:1243	prebiotic mixture	1227:1243	In addition, the fermented milk with prebiotic mixture was able to bind the virus and reduce its clearance.					
30669251	3	11	theme	Bifidobacterium	500:514	arg1	BbC50					527:531	BbC50	527:531	BbC50	527:531	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	3	11	theme	Bifidobacterium	500:514	arg1	C50					522:524	Bifidobacterium breve C50	500:524	Bifidobacterium breve C50 (BbC50)	500:532	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	1	12	theme	acute	267:271	arg1	diarrhea					273:280	acute diarrhea	267:280	acute diarrhea caused by the rotavirus (RV)	267:309	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	7	13	theme	prebiotic	1227:1235	arg1	mixture					1237:1243	prebiotic mixture	1227:1243	prebiotic mixture	1227:1243	In addition, the fermented milk with prebiotic mixture was able to bind the virus and reduce its clearance.					
30669251	6	14	theme	associated	1169:1178	arg1	diarrhea					1180:1187	the associated diarrhea	1165:1187	the associated diarrhea	1165:1187	Furthermore, a modulation of the immune response was observed, which might enhance the reduction of the associated diarrhea.					
30669251	3	15	theme	breve	516:520	arg1	BbC50					527:531	BbC50	527:531	BbC50	527:531	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	3	15	theme	breve	516:520	arg1	C50					522:524	Bifidobacterium breve C50	500:524	Bifidobacterium breve C50 (BbC50)	500:532	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	5	16	from	reduction	985:993	arg1	symptoms					1011:1018	the clinical symptoms	998:1018	the clinical symptoms of diarrhea	998:1030	The dietary intervention caused a reduction in the clinical symptoms of diarrhea, such as severity and incidence.					
30669251	2	17	theme	Other	312:316	arg1	modulators					329:338	Other microbiota modulators	312:338	Other microbiota modulators	312:338	Other microbiota modulators, such as postbiotics, produced during fermentation, might also have the potential to counteract RV infection.					
30669251	2	17	theme	Other	312:316	arg1	postbiotics					349:359	postbiotics	349:359	postbiotics	349:359	Other microbiota modulators, such as postbiotics, produced during fermentation, might also have the potential to counteract RV infection.					
30669251	0	18	from	Rats	45:48	arg1	Prevention					0:9	Prevention	0:9	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk	0:77	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture.					
30669251	4	19	theme	RV	768:769	arg1	model					784:788	An RV infected rat model	765:788	An RV infected rat model	765:788	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	8	20	from	milk	1356:1359	arg1	combination					1364:1374	combination	1364:1374	combination with the prebiotics used here	1364:1404	In conclusion, the postbiotic components in the fermented milk in combination with the prebiotics used here showed protective properties against RV infection.					
30669251	1	21	theme	certain	162:168	arg1	probiotics					170:179	certain probiotics	162:179	certain probiotics	162:179	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	0	22	theme	Prebiotic	96:104	arg1	Mixture					106:112	Prebiotic Mixture	96:112	Prebiotic Mixture	96:112	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture.					
30669251	4	23	used	used	794:797	arg2	model					784:788	An RV infected rat model	765:788	An RV infected rat model	765:788	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	3	24	dep	Streptococcus	538:550	arg1	thermophilus					552:563	thermophilus	552:563	thermophilus	552:563	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	2	25	theme	RV	436:437	arg1	infection					439:447	RV infection	436:447	RV infection	436:447	Other microbiota modulators, such as postbiotics, produced during fermentation, might also have the potential to counteract RV infection.					
30669251	4	26	theme	infectious	835:844	arg1	process					846:852	the infectious process	831:852	the infectious process	831:852	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	8	27	theme	postbiotic	1317:1326	arg1	components					1328:1337	the postbiotic components	1313:1337	the postbiotic components in the fermented milk in combination with the prebiotics used here	1313:1404	In conclusion, the postbiotic components in the fermented milk in combination with the prebiotics used here showed protective properties against RV infection.					
30669251	7	28	from	addition	1193:1200	arg1	milk					1217:1220	the fermented milk	1203:1220	the fermented milk with prebiotic mixture	1203:1243	In addition, the fermented milk with prebiotic mixture was able to bind the virus and reduce its clearance.					
30669251	7	28	from	addition	1193:1200	arg1	able					1249:1252	able	1249:1252	able	1249:1252	In addition, the fermented milk with prebiotic mixture was able to bind the virus and reduce its clearance.					
30669251	8	29	theme	RV	1443:1444	arg1	infection					1446:1454	RV infection	1443:1454	RV infection	1443:1454	In conclusion, the postbiotic components in the fermented milk in combination with the prebiotics used here showed protective properties against RV infection.					
30669251	5	30	theme	diarrhea	1023:1030	arg1	symptoms					1011:1018	the clinical symptoms	998:1018	the clinical symptoms of diarrhea	998:1030	The dietary intervention caused a reduction in the clinical symptoms of diarrhea, such as severity and incidence.					
30669251	4	31	theme	process	846:852	arg1	amelioration					815:826	the amelioration	811:826	the amelioration of the infectious process	811:852	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	4	31	theme	process	846:852	arg1	improvement					862:872	the improvement	858:872	the improvement of the immune response induced by the fermented milk with prebiotic mixture	858:948	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	0	32	theme	Diarrhea	24:31	arg1	Prevention					0:9	Prevention	0:9	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk	0:77	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture.					
30669251	7	33	from	able	1249:1252	arg1	addition					1193:1200	addition	1193:1200	addition	1193:1200	In addition, the fermented milk with prebiotic mixture was able to bind the virus and reduce its clearance.					
30669251	0	34	theme	Rotavirus	14:22	arg1	Diarrhea					24:31	Rotavirus Diarrhea	14:31	Rotavirus Diarrhea in Suckling Rats	14:48	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture.					
30669251	3	35	theme	intestinal	719:728	arg1	composition					741:751	the intestinal microbiota composition	715:751	the intestinal microbiota composition	715:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	5	36	theme	dietary	955:961	arg1	intervention					963:974	The dietary intervention	951:974	The dietary intervention	951:974	The dietary intervention caused a reduction in the clinical symptoms of diarrhea, such as severity and incidence.					
30669251	3	37	theme	mixture-short	594:606	arg1	potential					695:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential	582:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition	582:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	7	38	theme	fermented	1207:1215	arg1	milk					1217:1220	the fermented milk	1203:1220	the fermented milk with prebiotic mixture	1203:1243	In addition, the fermented milk with prebiotic mixture was able to bind the virus and reduce its clearance.					
30669251	7	38	theme	fermented	1207:1215	arg1	able					1249:1252	able	1249:1252	able	1249:1252	In addition, the fermented milk with prebiotic mixture was able to bind the virus and reduce its clearance.					
30669251	0	39	theme	Suckling	36:43	arg1	Rats					45:48	Suckling Rats	36:48	Suckling Rats	36:48	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture.					
30669251	3	40	theme	microbiota	730:739	arg1	composition					741:751	the intestinal microbiota composition	715:751	the intestinal microbiota composition	715:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	4	41	theme	fermented	912:920	arg1	milk					922:925	the fermented milk	908:925	the fermented milk with prebiotic mixture	908:948	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	3	42	theme	chain	608:612	arg1	potential					695:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential	582:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition	582:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	8	43	theme	fermented	1346:1354	arg1	milk					1356:1359	the fermented milk	1342:1359	the fermented milk in combination with the prebiotics used here	1342:1404	In conclusion, the postbiotic components in the fermented milk in combination with the prebiotics used here showed protective properties against RV infection.					
30669251	3	44	dep	potential	695:703	arg1	impact					708:713	impact	708:713	impact	708:713	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	8	45	theme	protective	1413:1422	arg1	properties					1424:1433	protective properties	1413:1433	protective properties	1413:1433	In conclusion, the postbiotic components in the fermented milk in combination with the prebiotics used here showed protective properties against RV infection.					
30669251	5	46	theme	clinical	1002:1009	arg1	symptoms					1011:1018	the clinical symptoms	998:1018	the clinical symptoms of diarrhea	998:1030	The dietary intervention caused a reduction in the clinical symptoms of diarrhea, such as severity and incidence.					
30669251	4	47	theme	prebiotic	932:940	arg1	mixture					942:948	prebiotic mixture	932:948	prebiotic mixture	932:948	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	3	48	theme	prebiotic	584:592	arg1	potential					695:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential	582:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition	582:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	6	49	theme	response	1105:1112	arg1	modulation					1080:1089	a modulation	1078:1089	a modulation of the immune response	1078:1112	Furthermore, a modulation of the immune response was observed, which might enhance the reduction of the associated diarrhea.					
30669251	3	50	theme	9:1	685:687	arg1	potential					695:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential	582:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition	582:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	3	51	theme	fructooligosaccharides	649:670	arg1	potential					695:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential	582:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition	582:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	3	52	theme	Streptococcus	538:550	arg1	St065					570:574	St065	570:574	St065	570:574	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	3	52	theme	Streptococcus	538:550	arg1	065					565:567	Streptococcus thermophilus 065	538:567	Streptococcus thermophilus 065 (St065)	538:575	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	1	53	theme	Several	115:121	arg1	prebiotics					185:194	prebiotics	185:194	prebiotics	185:194	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	1	53	theme	Several	115:121	arg1	probiotics					170:179	certain probiotics	162:179	certain probiotics	162:179	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	1	53	theme	Several	115:121	arg1	concepts					144:151	Several microbial modulatory concepts	115:151	Several microbial modulatory concepts	115:151	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	8	54	with	combination	1364:1374	arg1	prebiotics					1385:1394	the prebiotics	1381:1394	the prebiotics used here	1381:1404	In conclusion, the postbiotic components in the fermented milk in combination with the prebiotics used here showed protective properties against RV infection.					
30669251	4	55	theme	response	888:895	arg1	amelioration					815:826	the amelioration	811:826	the amelioration of the infectious process	811:852	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	4	55	theme	response	888:895	arg1	improvement					862:872	the improvement	858:872	the improvement of the immune response induced by the fermented milk with prebiotic mixture	858:948	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	6	56	theme	diarrhea	1180:1187	arg1	reduction					1152:1160	the reduction	1148:1160	the reduction of the associated diarrhea	1148:1187	Furthermore, a modulation of the immune response was observed, which might enhance the reduction of the associated diarrhea.					
30669251	4	57	theme	rat	780:782	arg1	model					784:788	An RV infected rat model	765:788	An RV infected rat model	765:788	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	1	58	theme	microbial	123:131	arg1	prebiotics					185:194	prebiotics	185:194	prebiotics	185:194	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	1	58	theme	microbial	123:131	arg1	probiotics					170:179	certain probiotics	162:179	certain probiotics	162:179	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	1	58	theme	microbial	123:131	arg1	concepts					144:151	Several microbial modulatory concepts	115:151	Several microbial modulatory concepts	115:151	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	1	59	theme	gastrointestinal	223:238	arg1	infections					240:249	gastrointestinal infections	223:249	gastrointestinal infections	223:249	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	4	60	theme	immune	881:886	arg1	response					888:895	the immune response	877:895	the immune response induced by the fermented milk with prebiotic mixture	877:948	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	2	61	contain	have	403:406	arg1	modulators					329:338	Other microbiota modulators	312:338	Other microbiota modulators	312:338	Other microbiota modulators, such as postbiotics, produced during fermentation, might also have the potential to counteract RV infection.					
30669251	2	61	contain	have	403:406	arg2	potential					412:420	the potential to counteract RV infection	408:447	the potential to counteract RV infection	408:447	Other microbiota modulators, such as postbiotics, produced during fermentation, might also have the potential to counteract RV infection.					
30669251	2	61	contain	have	403:406	arg1	postbiotics					349:359	postbiotics	349:359	postbiotics	349:359	Other microbiota modulators, such as postbiotics, produced during fermentation, might also have the potential to counteract RV infection.					
30669251	4	62	theme	infected	771:778	arg1	model					784:788	An RV infected rat model	765:788	An RV infected rat model	765:788	An RV infected rat model was used to evaluate the amelioration of the infectious process and the improvement of the immune response induced by the fermented milk with prebiotic mixture.					
30669251	1	63	theme	modulatory	133:142	arg1	prebiotics					185:194	prebiotics	185:194	prebiotics	185:194	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	1	63	theme	modulatory	133:142	arg1	probiotics					170:179	certain probiotics	162:179	certain probiotics	162:179	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	1	63	theme	modulatory	133:142	arg1	concepts					144:151	Several microbial modulatory concepts	115:151	Several microbial modulatory concepts	115:151	Several microbial modulatory concepts, such as certain probiotics and prebiotics, confer protection against gastrointestinal infections, among which is acute diarrhea caused by the rotavirus (RV).					
30669251	2	64	theme	microbiota	318:327	arg1	modulators					329:338	Other microbiota modulators	312:338	Other microbiota modulators	312:338	Other microbiota modulators, such as postbiotics, produced during fermentation, might also have the potential to counteract RV infection.					
30669251	2	64	theme	microbiota	318:327	arg1	postbiotics					349:359	postbiotics	349:359	postbiotics	349:359	Other microbiota modulators, such as postbiotics, produced during fermentation, might also have the potential to counteract RV infection.					
30669251	0	65	theme	Specific	55:62	arg1	Milk					74:77	a Specific Fermented Milk	53:77	a Specific Fermented Milk	53:77	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture.					
30669251	3	66	theme	chain	643:647	arg1	potential					695:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential	582:703	a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition	582:751	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	3	67	theme	this	462:465	arg1	light					453:457	light	453:457	light of this	453:465	In light of this, a fermented milk, made by using Bifidobacterium breve C50 (BbC50) and Streptococcus thermophilus 065 (St065) with a prebiotic mixture-short chain galactooligosaccharides/long chain fructooligosaccharides (scGOS/lcFOS 9:1)-with potential to impact the intestinal microbiota composition was tested.					
30669251	0	68	from	Diarrhea	24:31	arg1	Rats					45:48	Suckling Rats	36:48	Suckling Rats	36:48	Prevention of Rotavirus Diarrhea in Suckling Rats by a Specific Fermented Milk Concentrate with Prebiotic Mixture.					
29485888	6	0	theme	central	1065:1071	arg1	design					1083:1088	A central composite design	1063:1088	A central composite design of experiments	1063:1103	A central composite design of experiments was implemented using the formulation with the narrowest plume angle to determine the patient-specific angle for targeting the turbinate region in each individual.					
29485888	3	1	theme	targeted	662:669	arg1	delivery					682:689	targeted nasal drug delivery	662:689	targeted nasal drug delivery	662:689	The effect of patient-specific administration parameters was evaluated for their ability to overcome the barriers of targeted nasal drug delivery.					
29485888	10	2	theme	Effective	1705:1713	arg1	targeting					1725:1733	Effective turbinate targeting	1705:1733	Effective turbinate targeting of nasal sprays	1705:1749	Effective turbinate targeting of nasal sprays can be accomplished with the use of patient-specific administration parameters in individuals.					
29485888	1	3	theme	drug	250:253	arg1	delivery					255:262	drug delivery	250:262	drug delivery	250:262	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	7	4	theme	administration	1438:1451	arg1	angle					1453:1457	an administration angle	1435:1457	an administration angle of 30°, around 90% compared to about 73%	1435:1498	The use of the patient-specific angle with this formulation significantly increased the turbinate deposition efficiency compared to that found for all subjects using an administration angle of 30°, around 90% compared to about 73%.					
29485888	6	5	theme	narrowest	1152:1160	arg1	angle					1168:1172	the narrowest plume angle	1148:1172	the narrowest plume angle	1148:1172	A central composite design of experiments was implemented using the formulation with the narrowest plume angle to determine the patient-specific angle for targeting the turbinate region in each individual.					
29485888	10	6	theme	nasal	1738:1742	arg1	sprays					1744:1749	nasal sprays	1738:1749	nasal sprays	1738:1749	Effective turbinate targeting of nasal sprays can be accomplished with the use of patient-specific administration parameters in individuals.					
29485888	7	7	theme	30°	1462:1464	arg1	angle					1453:1457	an administration angle	1435:1457	an administration angle of 30°, around 90% compared to about 73%	1435:1498	The use of the patient-specific angle with this formulation significantly increased the turbinate deposition efficiency compared to that found for all subjects using an administration angle of 30°, around 90% compared to about 73%.					
29485888	4	8	theme	adult	831:835	arg1	subjects					837:844	five adult subjects	826:844	five adult subjects	826:844	The nasal spray deposition was evaluated in 10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects.					
29485888	8	9	from	decreases	1557:1565	arg1	angle					1589:1593	the administration angle	1570:1593	the administration angle	1570:1593	Generally, we found turbinate deposition increased with decreases in the administration angle.					
29485888	7	10	dep	that	1401:1404	arg1	found					1406:1410	found	1406:1410	found for all subjects using an administration angle of 30°, around 90% compared to about 73%	1406:1498	The use of the patient-specific angle with this formulation significantly increased the turbinate deposition efficiency compared to that found for all subjects using an administration angle of 30°, around 90% compared to about 73%.					
29485888	1	11	theme	cavity	350:355	arg1	valve					378:382	the nasal valve	368:382	the nasal valve	368:382	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	1	11	theme	cavity	350:355	arg1	features					328:335	the anatomical features	313:335	the anatomical features	313:335	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	1	11	theme	cavity	350:355	arg1	turbinate					388:396	turbinate	388:396	turbinate	388:396	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	0	12	theme	3D-Printed	133:142	arg1	Casts					158:162	3D-Printed Nasal Replica Casts	133:162	3D-Printed Nasal Replica Casts	133:162	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	5	13	theme	angles	1055:1060	arg1	variety					1038:1044	a variety	1036:1044	a variety of plume angles	1036:1060	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	13	theme	angles	1055:1060	arg1	angles					1055:1060	plume angles	1049:1060	plume angles	1049:1060	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	10	14	theme	administration	1804:1817	arg1	parameters					1819:1828	patient-specific administration parameters	1787:1828	patient-specific administration parameters	1787:1828	Effective turbinate targeting of nasal sprays can be accomplished with the use of patient-specific administration parameters in individuals.					
29485888	0	15	theme	Replica	150:156	arg1	Casts					158:162	3D-Printed Nasal Replica Casts	133:162	3D-Printed Nasal Replica Casts	133:162	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	1	16	theme	nasal	372:376	arg1	valve					378:382	the nasal valve	368:382	the nasal valve	368:382	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	6	17	with	formulation	1131:1141	arg1	angle					1168:1172	the narrowest plume angle	1148:1172	the narrowest plume angle	1148:1172	A central composite design of experiments was implemented using the formulation with the narrowest plume angle to determine the patient-specific angle for targeting the turbinate region in each individual.					
29485888	6	18	theme	patient-specific	1191:1206	arg1	angle					1208:1212	the patient-specific angle	1187:1212	the patient-specific angle for targeting the turbinate region in each individual	1187:1266	A central composite design of experiments was implemented using the formulation with the narrowest plume angle to determine the patient-specific angle for targeting the turbinate region in each individual.					
29485888	7	19	theme	turbinate	1357:1365	arg1	efficiency					1378:1387	the turbinate deposition efficiency	1353:1387	the turbinate deposition efficiency	1353:1387	The use of the patient-specific angle with this formulation significantly increased the turbinate deposition efficiency compared to that found for all subjects using an administration angle of 30°, around 90% compared to about 73%.					
29485888	4	20	theme	spray	702:706	arg1	deposition					708:717	The nasal spray deposition	692:717	The nasal spray deposition	692:717	The nasal spray deposition was evaluated in 10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects.					
29485888	7	21	with	angle	1301:1305	arg1	formulation					1317:1327	this formulation	1312:1327	this formulation	1312:1327	The use of the patient-specific angle with this formulation significantly increased the turbinate deposition efficiency compared to that found for all subjects using an administration angle of 30°, around 90% compared to about 73%.					
29485888	11	22	theme	nasal	2000:2004	arg1	devices					2006:2012	other nasal devices	1994:2012	other nasal devices	1994:2012	Further research is required to see if these parameters can be device-controlled for patients and if other regions can be effectively targeted with other nasal devices.					
29485888	5	23	theme	varying	898:904	arg1	concentrations					906:919	varying concentrations	898:919	varying concentrations of hypromellose	898:935	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	2	24	from	differences	496:506	arg1	gender					522:527	gender	522:527	gender	522:527	Furthermore, nasal cavity geometries and dimensions vary between individuals based on differences in their age, gender, and ethnicity.					
29485888	2	24	from	differences	496:506	arg1	ethnicity					534:542	ethnicity	534:542	ethnicity	534:542	Furthermore, nasal cavity geometries and dimensions vary between individuals based on differences in their age, gender, and ethnicity.					
29485888	2	24	from	differences	496:506	arg1	age					517:519	their age	511:519	their age	511:519	Furthermore, nasal cavity geometries and dimensions vary between individuals based on differences in their age, gender, and ethnicity.					
29485888	3	25	theme	parameters	591:600	arg1	effect					549:554	The effect	545:554	The effect of patient-specific administration parameters	545:600	The effect of patient-specific administration parameters was evaluated for their ability to overcome the barriers of targeted nasal drug delivery.					
29485888	0	26	theme	Administration	69:82	arg1	Parameters					84:93	Patient-Specific Administration Parameters	52:93	Patient-Specific Administration Parameters	52:93	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	1	27	theme	Effective	165:173	arg1	targeting					175:183	Effective targeting	165:183	Effective targeting of nasal spray deposition	165:209	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	10	28	from	use	1780:1782	arg1	individuals					1833:1843	individuals	1833:1843	individuals	1833:1843	Effective turbinate targeting of nasal sprays can be accomplished with the use of patient-specific administration parameters in individuals.					
29485888	4	29	theme	3D-printed	739:748	arg1	replicas					763:770	10 3D-printed nasal cavity replicas	736:770	10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects	736:844	The nasal spray deposition was evaluated in 10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects.					
29485888	4	30	theme	cavity	756:761	arg1	replicas					763:770	10 3D-printed nasal cavity replicas	736:770	10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects	736:844	The nasal spray deposition was evaluated in 10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects.					
29485888	0	31	theme	Nasal	106:110	arg1	Targeting					117:125	Nasal Drug Targeting	106:125	Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts	106:162	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	5	32	theme	model	955:959	arg1	spray					967:971	a model nasal spray	953:971	a model nasal spray	953:971	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	32	theme	model	955:959	arg1	solution					869:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	0	33	theme	Personalized	0:11	arg1	Medicine					13:20	Personalized Medicine	0:20	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.	0:163	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	8	34	theme	turbinate	1521:1529	arg1	deposition					1531:1540	turbinate deposition	1521:1540	turbinate deposition	1521:1540	Generally, we found turbinate deposition increased with decreases in the administration angle.					
29485888	7	35	theme	angle	1301:1305	arg1	use					1273:1275	The use	1269:1275	The use of the patient-specific angle with this formulation	1269:1327	The use of the patient-specific angle with this formulation significantly increased the turbinate deposition efficiency compared to that found for all subjects using an administration angle of 30°, around 90% compared to about 73%.					
29485888	5	36	theme	deposition	989:998	arg1	pattern					1000:1006	the deposition pattern	985:1006	the deposition pattern from formulations producing a variety of plume angles	985:1060	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	2	37	theme	cavity	429:434	arg1	geometries					436:445	nasal cavity geometries	423:445	nasal cavity geometries	423:445	Furthermore, nasal cavity geometries and dimensions vary between individuals based on differences in their age, gender, and ethnicity.					
29485888	8	38	dep	found	1515:1519	arg1	increased					1542:1550	increased	1542:1550	found turbinate deposition increased with decreases in the administration angle	1515:1593	Generally, we found turbinate deposition increased with decreases in the administration angle.					
29485888	9	39	theme	upper	1614:1618	arg1	regions					1620:1626	the upper regions	1610:1626	the upper regions of the replica	1610:1641	Deposition to the upper regions of the replica was poor with any formulation or administration angle tested.					
29485888	6	40	theme	composite	1073:1081	arg1	design					1083:1088	A central composite design	1063:1088	A central composite design of experiments	1063:1103	A central composite design of experiments was implemented using the formulation with the narrowest plume angle to determine the patient-specific angle for targeting the turbinate region in each individual.					
29485888	3	41	theme	delivery	682:689	arg1	barriers					650:657	the barriers	646:657	the barriers of targeted nasal drug delivery	646:689	The effect of patient-specific administration parameters was evaluated for their ability to overcome the barriers of targeted nasal drug delivery.					
29485888	10	42	theme	turbinate	1715:1723	arg1	targeting					1725:1733	Effective turbinate targeting	1705:1733	Effective turbinate targeting of nasal sprays	1705:1749	Effective turbinate targeting of nasal sprays can be accomplished with the use of patient-specific administration parameters in individuals.					
29485888	6	43	from	region	1242:1247	arg1	individual					1257:1266	each individual	1252:1266	each individual	1252:1266	A central composite design of experiments was implemented using the formulation with the narrowest plume angle to determine the patient-specific angle for targeting the turbinate region in each individual.					
29485888	4	44	theme	subjects	837:844	arg1	CT-scans					795:802	the CT-scans	791:802	the CT-scans of five pediatric and five adult subjects	791:844	The nasal spray deposition was evaluated in 10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects.					
29485888	3	45	theme	nasal	671:675	arg1	delivery					682:689	targeted nasal drug delivery	662:689	targeted nasal drug delivery	662:689	The effect of patient-specific administration parameters was evaluated for their ability to overcome the barriers of targeted nasal drug delivery.					
29485888	6	46	theme	plume	1162:1166	arg1	angle					1168:1172	the narrowest plume angle	1148:1172	the narrowest plume angle	1148:1172	A central composite design of experiments was implemented using the formulation with the narrowest plume angle to determine the patient-specific angle for targeting the turbinate region in each individual.					
29485888	1	47	dep	valve	378:382	arg1	structures					398:407	structures	398:407	structures	398:407	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	10	48	theme	sprays	1744:1749	arg1	targeting					1725:1733	Effective turbinate targeting	1705:1733	Effective turbinate targeting of nasal sprays	1705:1749	Effective turbinate targeting of nasal sprays can be accomplished with the use of patient-specific administration parameters in individuals.					
29485888	1	49	theme	nasal	188:192	arg1	deposition					200:209	nasal spray deposition	188:209	nasal spray deposition	188:209	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	5	50	from	formulations	1013:1024	arg1	pattern					1000:1006	the deposition pattern	985:1006	the deposition pattern from formulations producing a variety of plume angles	985:1060	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	4	51	theme	pediatric	812:820	arg1	CT-scans					795:802	the CT-scans	791:802	the CT-scans of five pediatric and five adult subjects	791:844	The nasal spray deposition was evaluated in 10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects.					
29485888	5	52	theme	Cromolyn	847:854	arg1	USP					879:881	USP	879:881	USP	879:881	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	52	theme	Cromolyn	847:854	arg1	solution					869:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	52	theme	Cromolyn	847:854	arg1	spray					967:971	a model nasal spray	953:971	a model nasal spray	953:971	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	6	53	theme	turbinate	1232:1240	arg1	region					1242:1247	the turbinate region	1228:1247	the turbinate region in each individual	1228:1266	A central composite design of experiments was implemented using the formulation with the narrowest plume angle to determine the patient-specific angle for targeting the turbinate region in each individual.					
29485888	1	54	theme	deposition	200:209	arg1	targeting					175:183	Effective targeting	165:183	Effective targeting of nasal spray deposition	165:209	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	0	55	theme	Nasal	144:148	arg1	Casts					158:162	3D-Printed Nasal Replica Casts	133:162	3D-Printed Nasal Replica Casts	133:162	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	5	56	theme	plume	1049:1053	arg1	angles					1055:1060	plume angles	1049:1060	plume angles	1049:1060	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	10	57	theme	parameters	1819:1828	arg1	use					1780:1782	the use	1776:1782	the use of patient-specific administration parameters in individuals	1776:1843	Effective turbinate targeting of nasal sprays can be accomplished with the use of patient-specific administration parameters in individuals.					
29485888	1	58	theme	spray	194:198	arg1	deposition					200:209	nasal spray deposition	188:209	nasal spray deposition	188:209	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	11	59	theme	Further	1846:1852	arg1	research					1854:1861	Further research	1846:1861	Further research	1846:1861	Further research is required to see if these parameters can be device-controlled for patients and if other regions can be effectively targeted with other nasal devices.					
29485888	10	60	theme	patient-specific	1787:1802	arg1	parameters					1819:1828	patient-specific administration parameters	1787:1828	patient-specific administration parameters	1787:1828	Effective turbinate targeting of nasal sprays can be accomplished with the use of patient-specific administration parameters in individuals.					
29485888	11	61	theme	other	1994:1998	arg1	devices					2006:2012	other nasal devices	1994:2012	other nasal devices	1994:2012	Further research is required to see if these parameters can be device-controlled for patients and if other regions can be effectively targeted with other nasal devices.					
29485888	5	62	theme	sodium	856:861	arg1	USP					879:881	USP	879:881	USP	879:881	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	62	theme	sodium	856:861	arg1	solution					869:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	62	theme	sodium	856:861	arg1	spray					967:971	a model nasal spray	953:971	a model nasal spray	953:971	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	7	63	theme	deposition	1367:1376	arg1	efficiency					1378:1387	the turbinate deposition efficiency	1353:1387	the turbinate deposition efficiency	1353:1387	The use of the patient-specific angle with this formulation significantly increased the turbinate deposition efficiency compared to that found for all subjects using an administration angle of 30°, around 90% compared to about 73%.					
29485888	4	64	theme	nasal	696:700	arg1	deposition					708:717	The nasal spray deposition	692:717	The nasal spray deposition	692:717	The nasal spray deposition was evaluated in 10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects.					
29485888	0	65	theme	Patient-Specific	52:67	arg1	Parameters					84:93	Patient-Specific Administration Parameters	52:93	Patient-Specific Administration Parameters	52:93	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	3	66	theme	administration	576:589	arg1	parameters					591:600	patient-specific administration parameters	559:600	patient-specific administration parameters	559:600	The effect of patient-specific administration parameters was evaluated for their ability to overcome the barriers of targeted nasal drug delivery.					
29485888	5	67	theme	nasal	863:867	arg1	USP					879:881	USP	879:881	USP	879:881	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	67	theme	nasal	863:867	arg1	solution					869:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	67	theme	nasal	863:867	arg1	spray					967:971	a model nasal spray	953:971	a model nasal spray	953:971	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	0	68	theme	Parameters	84:93	arg1	Use					45:47	The Use	41:47	The Use of Patient-Specific Administration Parameters	41:93	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	0	69	from	Medicine	13:20	arg1	Delivery					31:38	Nasal Delivery	25:38	Nasal Delivery	25:38	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	1	70	theme	anatomical	317:326	arg1	valve					378:382	the nasal valve	368:382	the nasal valve	368:382	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	1	70	theme	anatomical	317:326	arg1	features					328:335	the anatomical features	313:335	the anatomical features	313:335	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	1	70	theme	anatomical	317:326	arg1	turbinate					388:396	turbinate	388:396	turbinate	388:396	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	0	71	theme	Drug	112:115	arg1	Targeting					117:125	Nasal Drug Targeting	106:125	Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts	106:162	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	4	72	theme	nasal	750:754	arg1	replicas					763:770	10 3D-printed nasal cavity replicas	736:770	10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects	736:844	The nasal spray deposition was evaluated in 10 3D-printed nasal cavity replicas developed based on the CT-scans of five pediatric and five adult subjects.					
29485888	1	73	theme	nasal	344:348	arg1	cavity					350:355	the nasal cavity	340:355	the nasal cavity	340:355	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	9	74	theme	replica	1635:1641	arg1	regions					1620:1626	the upper regions	1610:1626	the upper regions of the replica	1610:1641	Deposition to the upper regions of the replica was poor with any formulation or administration angle tested.					
29485888	0	75	theme	Nasal	25:29	arg1	Delivery					31:38	Nasal Delivery	25:38	Nasal Delivery	25:38	Personalized Medicine in Nasal Delivery: The Use of Patient-Specific Administration Parameters To Improve Nasal Drug Targeting Using 3D-Printed Nasal Replica Casts.					
29485888	5	76	theme	hypromellose	924:935	arg1	concentrations					906:919	varying concentrations	898:919	varying concentrations of hypromellose	898:935	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	7	77	theme	patient-specific	1284:1299	arg1	angle					1301:1305	the patient-specific angle	1280:1305	the patient-specific angle with this formulation	1280:1327	The use of the patient-specific angle with this formulation significantly increased the turbinate deposition efficiency compared to that found for all subjects using an administration angle of 30°, around 90% compared to about 73%.					
29485888	11	78	theme	other	1947:1951	arg1	regions					1953:1959	other regions	1947:1959	other regions	1947:1959	Further research is required to see if these parameters can be device-controlled for patients and if other regions can be effectively targeted with other nasal devices.					
29485888	9	79	dep	formulation	1661:1671	arg1	any					1657:1659	any	1657:1659	any	1657:1659	Deposition to the upper regions of the replica was poor with any formulation or administration angle tested.					
29485888	9	79	dep	formulation	1661:1671	arg1	angle					1691:1695	angle	1691:1695	angle	1691:1695	Deposition to the upper regions of the replica was poor with any formulation or administration angle tested.					
29485888	5	80	used	utilized	941:948	arg2	USP					879:881	USP	879:881	USP	879:881	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	80	used	utilized	941:948	arg2	spray					967:971	a model nasal spray	953:971	a model nasal spray	953:971	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	80	used	utilized	941:948	arg2	solution					869:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	2	81	theme	nasal	423:427	arg1	geometries					436:445	nasal cavity geometries	423:445	nasal cavity geometries	423:445	Furthermore, nasal cavity geometries and dimensions vary between individuals based on differences in their age, gender, and ethnicity.					
29485888	8	82	theme	administration	1574:1587	arg1	angle					1589:1593	the administration angle	1570:1593	the administration angle	1570:1593	Generally, we found turbinate deposition increased with decreases in the administration angle.					
29485888	5	83	theme	nasal	961:965	arg1	spray					967:971	a model nasal spray	953:971	a model nasal spray	953:971	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	5	83	theme	nasal	961:965	arg1	solution					869:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution	847:876	Cromolyn sodium nasal solution, USP, modified with varying concentrations of hypromellose was utilized as a model nasal spray to evaluate the deposition pattern from formulations producing a variety of plume angles.					
29485888	3	84	theme	patient-specific	559:574	arg1	parameters					591:600	patient-specific administration parameters	559:600	patient-specific administration parameters	559:600	The effect of patient-specific administration parameters was evaluated for their ability to overcome the barriers of targeted nasal drug delivery.					
29485888	6	85	theme	experiments	1093:1103	arg1	design					1083:1088	A central composite design	1063:1088	A central composite design of experiments	1063:1103	A central composite design of experiments was implemented using the formulation with the narrowest plume angle to determine the patient-specific angle for targeting the turbinate region in each individual.					
29485888	1	86	dep	local	225:229	arg1	delivery					255:262	drug delivery	250:262	drug delivery	250:262	Effective targeting of nasal spray deposition could improve local, systemic, and CNS drug delivery; however, this has proven to be difficult due to the anatomical features of the nasal cavity, including the nasal valve and turbinate structures.					
29485888	3	87	theme	drug	677:680	arg1	delivery					682:689	targeted nasal drug delivery	662:689	targeted nasal drug delivery	662:689	The effect of patient-specific administration parameters was evaluated for their ability to overcome the barriers of targeted nasal drug delivery.					
29485888	9	88	with	poor	1647:1650	arg1	administration					1676:1689	administration	1676:1689	administration	1676:1689	Deposition to the upper regions of the replica was poor with any formulation or administration angle tested.					
29485888	9	88	with	poor	1647:1650	arg1	formulation					1661:1671	formulation	1661:1671	formulation	1661:1671	Deposition to the upper regions of the replica was poor with any formulation or administration angle tested.					
31462656	0	0	theme	Triple	57:62	arg1	Cancer					80:85	Triple Negative Breast Cancer	57:85	Triple Negative Breast Cancer	57:85	Discovery of HSPG2 (Perlecan) as a Therapeutic Target in Triple Negative Breast Cancer.					
31462656	7	1	theme	natural	1054:1060	arg1	killer					1062:1067	natural killer	1054:1067	natural killer cell-mediated antibody-dependent cell cytotoxicity	1054:1118	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	7	2	theme	negative	942:949	arg1	tumors					962:967	triple negative MDA-MB-231 tumors	935:967	triple negative MDA-MB-231 tumors	935:967	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	3	3	theme	human	443:447	arg1	antibodies					449:458	two human antibodies	439:458	two human antibodies that bind to tumor cells with a metastatic TNBC phenotype	439:516	Using a phage display-based whole cell biopanning procedure, we developed two human antibodies that bind to tumor cells with a metastatic TNBC phenotype.					
31462656	5	4	theme	significant	717:727	arg1	expression					742:751	significant cell surface expression	717:751	significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions	717:806	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	7	5	theme	killer	1062:1067	arg1	cytotoxicity					1107:1118	natural killer cell-mediated antibody-dependent cell cytotoxicity	1054:1118	natural killer cell-mediated antibody-dependent cell cytotoxicity	1054:1118	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	1	6	theme	significant	121:131	arg1	advances					133:140	significant advances	121:140	significant advances in the treatment of breast cancer resulting in remarkably high survival rates	121:218	In recent years, there have been significant advances in the treatment of breast cancer resulting in remarkably high survival rates.					
31462656	8	7	theme	mouse	1190:1194	arg1	splenocytes					1196:1206	mouse splenocytes	1190:1206	mouse splenocytes	1190:1206	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	0	8	theme	Breast	73:78	arg1	Cancer					80:85	Triple Negative Breast Cancer	57:85	Triple Negative Breast Cancer	57:85	Discovery of HSPG2 (Perlecan) as a Therapeutic Target in Triple Negative Breast Cancer.					
31462656	6	9	theme	poor	858:861	arg1	survival					863:870	poor survival	858:870	poor survival in TNBC	858:878	Further, higher HSPG2 expression correlated with poor survival in TNBC.					
31462656	9	10	from	target	1310:1315	arg1	TNBC					1331:1334	metastatic TNBC	1320:1334	metastatic TNBC	1320:1334	These results suggest that HSPG2 is a promising target in metastatic TNBC and HSPG2-targeted antibodies could represent a potentially novel class of targeted therapeutics for TNBC.					
31462656	2	11	theme	metastatic	252:261	arg1	TNBC					294:297	TNBC	294:297	TNBC	294:297	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	2	11	theme	metastatic	252:261	arg1	cancer					286:291	metastatic triple negative breast cancer	252:291	metastatic triple negative breast cancer (TNBC)	252:298	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	3	12	theme	metastatic	492:501	arg1	phenotype					508:516	a metastatic TNBC phenotype	490:516	a metastatic TNBC phenotype	490:516	Using a phage display-based whole cell biopanning procedure, we developed two human antibodies that bind to tumor cells with a metastatic TNBC phenotype.					
31462656	0	13	theme	Negative	64:71	arg1	Cancer					80:85	Triple Negative Breast Cancer	57:85	Triple Negative Breast Cancer	57:85	Discovery of HSPG2 (Perlecan) as a Therapeutic Target in Triple Negative Breast Cancer.					
31462656	4	14	theme	HSPG2	562:566	arg1	protein					579:585	HSPG2 (perlecan) protein	562:585	HSPG2 (perlecan) protein	562:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	8	15	theme	blood	1229:1233	arg1	PBMCs					1254:1258	PBMCs	1254:1258	PBMCs	1254:1258	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	8	15	theme	blood	1229:1233	arg1	cells					1247:1251	human peripheral blood mononuclear cells	1212:1251	human peripheral blood mononuclear cells (PBMCs)	1212:1259	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	2	16	theme	markers	356:362	arg1	lack					327:330	a lack	325:330	a lack of identifiable, unique markers	325:362	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	8	17	dep	in	1168:1169	arg1	vitro					1171:1175	vitro	1171:1175	vitro	1171:1175	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	8	18	theme	human	1212:1216	arg1	PBMCs					1254:1258	PBMCs	1254:1258	PBMCs	1254:1258	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	8	18	theme	human	1212:1216	arg1	cells					1247:1251	human peripheral blood mononuclear cells	1212:1251	human peripheral blood mononuclear cells (PBMCs)	1212:1259	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	2	19	theme	identifiable	335:346	arg1	markers					356:362	identifiable, unique markers	335:362	identifiable, unique markers	335:362	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	5	20	theme	patient	685:691	arg1	samples					700:706	patient tissue samples	685:706	patient tissue samples	685:706	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	7	21	theme	cell	1102:1105	arg1	cytotoxicity					1107:1118	natural killer cell-mediated antibody-dependent cell cytotoxicity	1054:1118	natural killer cell-mediated antibody-dependent cell cytotoxicity	1054:1118	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	7	22	theme	affinity-matured	885:900	arg1	antibody					902:909	The affinity-matured antibody	881:909	The affinity-matured antibody	881:909	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	2	23	theme	treatment	230:238	arg1	options					240:246	treatment options	230:246	treatment options for metastatic triple negative breast cancer (TNBC)	230:298	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	3	24	theme	display-based	379:391	arg1	procedure					415:423	a phage display-based whole cell biopanning procedure	371:423	a phage display-based whole cell biopanning procedure	371:423	Using a phage display-based whole cell biopanning procedure, we developed two human antibodies that bind to tumor cells with a metastatic TNBC phenotype.					
31462656	5	25	theme	HSPG2	756:760	arg1	expression					742:751	significant cell surface expression	717:751	significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions	717:806	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	6	26	theme	HSPG2	825:829	arg1	expression					831:840	higher HSPG2 expression	818:840	higher HSPG2 expression	818:840	Further, higher HSPG2 expression correlated with poor survival in TNBC.					
31462656	6	27	from	survival	863:870	arg1	TNBC					875:878	TNBC	875:878	TNBC	875:878	Further, higher HSPG2 expression correlated with poor survival in TNBC.					
31462656	3	28	theme	TNBC	503:506	arg1	phenotype					508:516	a metastatic TNBC phenotype	490:516	a metastatic TNBC phenotype	490:516	Using a phage display-based whole cell biopanning procedure, we developed two human antibodies that bind to tumor cells with a metastatic TNBC phenotype.					
31462656	4	29	theme	cognate	594:600	arg1	antigen					615:621	the cognate cell surface antigen	590:621	the cognate cell surface antigen bound by the antibody	590:643	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	4	29	theme	cognate	594:600	arg1	domain					550:555	domain 1	550:557	domain 1 of HSPG2 (perlecan) protein	550:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	4	29	theme	cognate	594:600	arg1	protein					579:585	HSPG2 (perlecan) protein	562:585	HSPG2 (perlecan) protein	562:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	8	30	theme	action	1139:1144	arg1	mechanism					1126:1134	This mechanism	1121:1134	This mechanism of action	1121:1144	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	7	31	theme	cytotoxicity	1107:1118	arg1	role					1046:1049	the potential role	1032:1049	the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity	1032:1118	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	2	32	theme	breast	279:284	arg1	TNBC					294:297	TNBC	294:297	TNBC	294:297	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	2	32	theme	breast	279:284	arg1	cancer					286:291	metastatic triple negative breast cancer	252:291	metastatic triple negative breast cancer (TNBC)	252:298	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	8	33	theme	peripheral	1218:1227	arg1	PBMCs					1254:1258	PBMCs	1254:1258	PBMCs	1254:1258	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	8	33	theme	peripheral	1218:1227	arg1	cells					1247:1251	human peripheral blood mononuclear cells	1212:1251	human peripheral blood mononuclear cells (PBMCs)	1212:1259	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	9	34	theme	therapeutics	1420:1431	arg1	class					1402:1406	a potentially novel class	1382:1406	a potentially novel class of targeted therapeutics for TNBC	1382:1440	These results suggest that HSPG2 is a promising target in metastatic TNBC and HSPG2-targeted antibodies could represent a potentially novel class of targeted therapeutics for TNBC.					
31462656	5	35	theme	metastatic	789:798	arg1	lesions					800:806	metastatic lesions	789:806	metastatic lesions	789:806	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	2	36	theme	negative	270:277	arg1	TNBC					294:297	TNBC	294:297	TNBC	294:297	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	2	36	theme	negative	270:277	arg1	cancer					286:291	metastatic triple negative breast cancer	252:291	metastatic triple negative breast cancer (TNBC)	252:298	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	3	37	theme	biopanning	404:413	arg1	procedure					415:423	a phage display-based whole cell biopanning procedure	371:423	a phage display-based whole cell biopanning procedure	371:423	Using a phage display-based whole cell biopanning procedure, we developed two human antibodies that bind to tumor cells with a metastatic TNBC phenotype.					
31462656	0	38	theme	HSPG2	13:17	arg1	Discovery					0:8	Discovery	0:8	Discovery of HSPG2 (Perlecan) as a Therapeutic Target in Triple Negative Breast Cancer.	0:86	Discovery of HSPG2 (Perlecan) as a Therapeutic Target in Triple Negative Breast Cancer.					
31462656	6	39	theme	higher	818:823	arg1	expression					831:840	higher HSPG2 expression	818:840	higher HSPG2 expression	818:840	Further, higher HSPG2 expression correlated with poor survival in TNBC.					
31462656	2	40	theme	triple	263:268	arg1	TNBC					294:297	TNBC	294:297	TNBC	294:297	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	2	40	theme	triple	263:268	arg1	cancer					286:291	metastatic triple negative breast cancer	252:291	metastatic triple negative breast cancer (TNBC)	252:298	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	9	41	theme	novel	1396:1400	arg1	class					1402:1406	a potentially novel class	1382:1406	a potentially novel class of targeted therapeutics for TNBC	1382:1440	These results suggest that HSPG2 is a promising target in metastatic TNBC and HSPG2-targeted antibodies could represent a potentially novel class of targeted therapeutics for TNBC.					
31462656	1	42	theme	breast	162:167	arg1	cancer					169:174	breast cancer	162:174	breast cancer resulting in remarkably high survival rates	162:218	In recent years, there have been significant advances in the treatment of breast cancer resulting in remarkably high survival rates.					
31462656	5	43	theme	cell	729:732	arg1	expression					742:751	significant cell surface expression	717:751	significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions	717:806	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	9	44	theme	promising	1300:1308	arg1	HSPG2					1289:1293	HSPG2	1289:1293	HSPG2	1289:1293	These results suggest that HSPG2 is a promising target in metastatic TNBC and HSPG2-targeted antibodies could represent a potentially novel class of targeted therapeutics for TNBC.					
31462656	9	44	theme	promising	1300:1308	arg1	target					1310:1315	a promising target	1298:1315	a promising target in metastatic TNBC	1298:1334	These results suggest that HSPG2 is a promising target in metastatic TNBC and HSPG2-targeted antibodies could represent a potentially novel class of targeted therapeutics for TNBC.					
31462656	3	45	theme	whole	393:397	arg1	procedure					415:423	a phage display-based whole cell biopanning procedure	371:423	a phage display-based whole cell biopanning procedure	371:423	Using a phage display-based whole cell biopanning procedure, we developed two human antibodies that bind to tumor cells with a metastatic TNBC phenotype.					
31462656	1	46	theme	cancer	169:174	arg1	treatment					149:157	the treatment	145:157	the treatment of breast cancer resulting in remarkably high survival rates	145:218	In recent years, there have been significant advances in the treatment of breast cancer resulting in remarkably high survival rates.					
31462656	3	47	theme	phage	373:377	arg1	procedure					415:423	a phage display-based whole cell biopanning procedure	371:423	a phage display-based whole cell biopanning procedure	371:423	Using a phage display-based whole cell biopanning procedure, we developed two human antibodies that bind to tumor cells with a metastatic TNBC phenotype.					
31462656	5	48	theme	surface	734:740	arg1	expression					742:751	significant cell surface expression	717:751	significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions	717:806	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	0	49	from	Target	47:52	arg1	Cancer					80:85	Triple Negative Breast Cancer	57:85	Triple Negative Breast Cancer	57:85	Discovery of HSPG2 (Perlecan) as a Therapeutic Target in Triple Negative Breast Cancer.					
31462656	7	50	theme	nude	992:995	arg1	mice					997:1000	nude mice	992:1000	nude mice	992:1000	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	7	51	theme	greater	974:980	arg1	extent					982:987	a greater extent	972:987	a greater extent	972:987	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	3	52	theme	cell	399:402	arg1	procedure					415:423	a phage display-based whole cell biopanning procedure	371:423	a phage display-based whole cell biopanning procedure	371:423	Using a phage display-based whole cell biopanning procedure, we developed two human antibodies that bind to tumor cells with a metastatic TNBC phenotype.					
31462656	7	53	theme	cell-mediated	1069:1081	arg1	cytotoxicity					1107:1118	natural killer cell-mediated antibody-dependent cell cytotoxicity	1054:1118	natural killer cell-mediated antibody-dependent cell cytotoxicity	1054:1118	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	5	54	theme	Immunohistochemistry	646:665	arg1	studies					667:673	Immunohistochemistry studies	646:673	Immunohistochemistry studies utilizing patient tissue samples	646:706	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	8	55	theme	in	1168:1169	arg1	assays					1177:1182	in vitro assays	1168:1182	in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs)	1168:1259	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	7	56	theme	NSG	1010:1012	arg1	mice					1014:1017	NSG mice	1010:1017	NSG mice	1010:1017	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	9	57	theme	metastatic	1320:1329	arg1	TNBC					1331:1334	metastatic TNBC	1320:1334	metastatic TNBC	1320:1334	These results suggest that HSPG2 is a promising target in metastatic TNBC and HSPG2-targeted antibodies could represent a potentially novel class of targeted therapeutics for TNBC.					
31462656	7	58	theme	potential	1036:1044	arg1	role					1046:1049	the potential role	1032:1049	the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity	1032:1118	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	1	59	theme	recent	91:96	arg1	years					98:102	recent years	91:102	recent years	91:102	In recent years, there have been significant advances in the treatment of breast cancer resulting in remarkably high survival rates.					
31462656	7	60	theme	antibody-dependent	1083:1100	arg1	cytotoxicity					1107:1118	natural killer cell-mediated antibody-dependent cell cytotoxicity	1054:1118	natural killer cell-mediated antibody-dependent cell cytotoxicity	1054:1118	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	7	61	theme	triple	935:940	arg1	tumors					962:967	triple negative MDA-MB-231 tumors	935:967	triple negative MDA-MB-231 tumors	935:967	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	8	62	theme	mononuclear	1235:1245	arg1	PBMCs					1254:1258	PBMCs	1254:1258	PBMCs	1254:1258	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	8	62	theme	mononuclear	1235:1245	arg1	cells					1247:1251	human peripheral blood mononuclear cells	1212:1251	human peripheral blood mononuclear cells (PBMCs)	1212:1259	This mechanism of action was confirmed through in vitro assays using mouse splenocytes and human peripheral blood mononuclear cells (PBMCs).					
31462656	3	63	theme	tumor	473:477	arg1	cells					479:483	tumor cells	473:483	tumor cells	473:483	Using a phage display-based whole cell biopanning procedure, we developed two human antibodies that bind to tumor cells with a metastatic TNBC phenotype.					
31462656	7	64	theme	MDA-MB-231	951:960	arg1	tumors					962:967	triple negative MDA-MB-231 tumors	935:967	triple negative MDA-MB-231 tumors	935:967	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	9	65	theme	targeted	1411:1418	arg1	therapeutics					1420:1431	targeted therapeutics	1411:1431	targeted therapeutics	1411:1431	These results suggest that HSPG2 is a promising target in metastatic TNBC and HSPG2-targeted antibodies could represent a potentially novel class of targeted therapeutics for TNBC.					
31462656	9	66	theme	HSPG2-targeted	1340:1353	arg1	antibodies					1355:1364	HSPG2-targeted antibodies	1340:1364	HSPG2-targeted antibodies	1340:1364	These results suggest that HSPG2 is a promising target in metastatic TNBC and HSPG2-targeted antibodies could represent a potentially novel class of targeted therapeutics for TNBC.					
31462656	5	67	theme	tissue	693:698	arg1	samples					700:706	patient tissue samples	685:706	patient tissue samples	685:706	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	4	68	theme	perlecan	569:576	arg1	protein					579:585	HSPG2 (perlecan) protein	562:585	HSPG2 (perlecan) protein	562:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	2	69	dep	identifiable	335:346	arg1	unique					349:354	unique	349:354	unique	349:354	However, treatment options for metastatic triple negative breast cancer (TNBC) are quite limited due to a lack of identifiable, unique markers.					
31462656	7	70	theme	tumors	962:967	arg1	growth					925:930	the growth	921:930	the growth of triple negative MDA-MB-231 tumors to a greater extent	921:987	The affinity-matured antibody inhibited the growth of triple negative MDA-MB-231 tumors to a greater extent in nude mice than in NSG mice, pointing to the potential role of natural killer cell-mediated antibody-dependent cell cytotoxicity.					
31462656	4	71	theme	surface	607:613	arg1	antigen					615:621	the cognate cell surface antigen	590:621	the cognate cell surface antigen bound by the antibody	590:643	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	4	71	theme	surface	607:613	arg1	domain					550:555	domain 1	550:557	domain 1 of HSPG2 (perlecan) protein	550:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	4	71	theme	surface	607:613	arg1	protein					579:585	HSPG2 (perlecan) protein	562:585	HSPG2 (perlecan) protein	562:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	4	72	theme	protein	579:585	arg1	antigen					615:621	the cognate cell surface antigen	590:621	the cognate cell surface antigen bound by the antibody	590:643	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	4	72	theme	protein	579:585	arg1	domain					550:555	domain 1	550:557	domain 1 of HSPG2 (perlecan) protein	550:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	4	72	theme	protein	579:585	arg1	protein					579:585	HSPG2 (perlecan) protein	562:585	HSPG2 (perlecan) protein	562:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	1	73	theme	high	200:203	arg1	rates					214:218	remarkably high survival rates	189:218	remarkably high survival rates	189:218	In recent years, there have been significant advances in the treatment of breast cancer resulting in remarkably high survival rates.					
31462656	0	74	theme	Therapeutic	35:45	arg1	Target					47:52	a Therapeutic Target	33:52	a Therapeutic Target in Triple Negative Breast Cancer	33:85	Discovery of HSPG2 (Perlecan) as a Therapeutic Target in Triple Negative Breast Cancer.					
31462656	5	75	from	expression	742:751	arg1	tumors					778:783	primary tumors	770:783	primary tumors	770:783	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	5	75	from	expression	742:751	arg1	lesions					800:806	metastatic lesions	789:806	metastatic lesions	789:806	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	5	76	theme	primary	770:776	arg1	tumors					778:783	primary tumors	770:783	primary tumors	770:783	Immunohistochemistry studies utilizing patient tissue samples revealed significant cell surface expression of HSPG2 in both primary tumors and metastatic lesions.					
31462656	1	77	from	advances	133:140	arg1	years					98:102	recent years	91:102	recent years	91:102	In recent years, there have been significant advances in the treatment of breast cancer resulting in remarkably high survival rates.					
31462656	1	77	from	advances	133:140	arg1	treatment					149:157	the treatment	145:157	the treatment of breast cancer resulting in remarkably high survival rates	145:218	In recent years, there have been significant advances in the treatment of breast cancer resulting in remarkably high survival rates.					
31462656	4	78	theme	cell	602:605	arg1	antigen					615:621	the cognate cell surface antigen	590:621	the cognate cell surface antigen bound by the antibody	590:643	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	4	78	theme	cell	602:605	arg1	domain					550:555	domain 1	550:557	domain 1 of HSPG2 (perlecan) protein	550:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	4	78	theme	cell	602:605	arg1	protein					579:585	HSPG2 (perlecan) protein	562:585	HSPG2 (perlecan) protein	562:585	Our studies further identified domain 1 of HSPG2 (perlecan) protein as the cognate cell surface antigen bound by the antibody.					
31462656	1	79	theme	survival	205:212	arg1	rates					214:218	remarkably high survival rates	189:218	remarkably high survival rates	189:218	In recent years, there have been significant advances in the treatment of breast cancer resulting in remarkably high survival rates.					
30372014	0	0	theme	Composite	92:100	arg1	Hydrogel					102:109	Bacterial Nanocellulose Composite Hydrogel	68:109	Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration	68:133	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	8	1	theme	potential	1462:1470	arg1	hydrogel					1448:1455	the BNC/AA hydrogel	1437:1455	the BNC/AA hydrogel	1437:1455	The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.					
30372014	8	1	theme	potential	1462:1470	arg1	biomaterial					1472:1482	a potential biomaterial	1460:1482	a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing	1460:1590	The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.					
30372014	8	1	theme	potential	1462:1470	arg1	material					1525:1532	a wound-dressing material	1508:1532	a wound-dressing material	1508:1532	The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.					
30372014	5	2	theme	similar	1045:1051	arg1	attachment					1058:1067	a similar cell attachment	1043:1067	a similar cell attachment	1043:1067	However, water vapor transmission rate, pore size, hydrophilicity (semidry), and topography were similar between all formulations, leading to a similar cell attachment and proliferation profile.					
30372014	2	3	theme	hydrogels	367:375	arg1	properties					307:316	the properties	303:316	the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses	303:452	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	0	4	theme	Nanocellulose	78:90	arg1	Hydrogel					102:109	Bacterial Nanocellulose Composite Hydrogel	68:109	Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration	68:133	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	3	5	theme	wound	684:688	arg1	material					699:706	wound dressing material	684:706	wound dressing material	684:706	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	1	6	theme	cells	173:177	arg1	interaction					158:168	the interaction	154:168	the interaction of cells with biomaterials	154:195	The evaluation of the interaction of cells with biomaterials is fundamental to establish the suitability of the biomaterial for a specific application.					
30372014	1	7	theme	specific	266:273	arg1	application					275:285	a specific application	264:285	a specific application	264:285	The evaluation of the interaction of cells with biomaterials is fundamental to establish the suitability of the biomaterial for a specific application.					
30372014	3	8	theme	dressing	690:697	arg1	material					699:706	wound dressing material	684:706	wound dressing material	684:706	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	6	9	theme	cellular	1219:1226	arg1	migration					1228:1236	restricted cellular migration	1208:1236	restricted cellular migration	1208:1236	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	5	10	theme	vapor	916:920	arg1	rate					935:938	water vapor transmission rate	910:938	water vapor transmission rate	910:938	However, water vapor transmission rate, pore size, hydrophilicity (semidry), and topography were similar between all formulations, leading to a similar cell attachment and proliferation profile.					
30372014	3	11	theme	cellular	573:580	arg1	levels					596:601	the cellular and molecular levels	569:601	the cellular and molecular levels	569:601	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	1	12	with	interaction	158:168	arg1	biomaterials					184:195	biomaterials	184:195	biomaterials	184:195	The evaluation of the interaction of cells with biomaterials is fundamental to establish the suitability of the biomaterial for a specific application.					
30372014	6	13	theme	rapid	1145:1149	arg1	adhesion					1156:1163	rapid cell adhesion	1145:1163	rapid cell adhesion	1145:1163	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	5	14	theme	transmission	922:933	arg1	rate					935:938	water vapor transmission rate	910:938	water vapor transmission rate	910:938	However, water vapor transmission rate, pore size, hydrophilicity (semidry), and topography were similar between all formulations, leading to a similar cell attachment and proliferation profile.					
30372014	8	15	theme	wound	1578:1582	arg1	healing					1584:1590	wound healing	1578:1590	wound healing	1578:1590	The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.					
30372014	3	16	theme	hydrogel	488:495	arg1	properties					497:506	these hydrogel properties	482:506	these hydrogel properties	482:506	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	2	17	theme	AA	408:409	arg1	ratios					411:416	AA ratios	408:416	AA ratios	408:416	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	0	18	theme	Tissue	115:120	arg1	Regeneration					122:133	Tissue Regeneration	115:133	Tissue Regeneration	115:133	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	6	19	theme	cellular	1103:1110	arg1	level					1112:1116	the cellular level	1099:1116	the cellular level	1099:1116	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	3	20	theme	fibroblasts	547:557	arg1	behavior					522:529	the behavior	518:529	the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels	518:601	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	2	21	theme	bacterial	321:329	arg1	hydrogels					367:375	bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels	321:375	bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses	321:452	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	8	22	theme	healing	1584:1590	arg1	acceleration					1562:1573	the acceleration	1558:1573	the acceleration of wound healing	1558:1590	The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.					
30372014	2	23	theme	BNC	401:403	arg1	doses					448:452	varying BNC to AA ratios and electron-beam irradiation doses	393:452	varying BNC to AA ratios and electron-beam irradiation doses	393:452	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	0	24	theme	Cellular	0:7	arg1	Interaction					23:33	Cellular and Molecular Interaction	0:33	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration	0:133	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	3	25	theme	molecular	586:594	arg1	levels					596:601	the cellular and molecular levels	569:601	the cellular and molecular levels	569:601	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	3	26	from	levels	596:601	arg1	behavior					522:529	the behavior	518:529	the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels	518:601	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	2	27	theme	varying	393:399	arg1	BNC					401:403	varying BNC	393:403	varying BNC to AA ratios	393:416	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	4	28	theme	adhesive	729:736	arg1	wet					745:747	wet	745:747	wet	745:747	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	28	theme	adhesive	729:736	arg1	force					738:742	adhesive force	729:742	adhesive force (wet)	729:748	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	7	29	theme	wound-healing	1330:1342	arg1	IL6					1351:1353	IL6	1351:1353	IL6	1351:1353	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	29	theme	wound-healing	1330:1342	arg1	CTSK					1368:1371	CTSK	1368:1371	CTSK	1368:1371	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	29	theme	wound-healing	1330:1342	arg1	FGF7					1374:1377	FGF7	1374:1377	FGF7	1374:1377	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	29	theme	wound-healing	1330:1342	arg1	COX2					1395:1398	COX2	1395:1398	COX2	1395:1398	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	29	theme	wound-healing	1330:1342	arg1	IL10					1356:1359	IL10	1356:1359	IL10	1356:1359	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	29	theme	wound-healing	1330:1342	arg1	MMP2					1362:1365	MMP2	1362:1365	MMP2	1362:1365	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	29	theme	wound-healing	1330:1342	arg1	GM-CSF					1380:1385	GM-CSF	1380:1385	GM-CSF	1380:1385	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	29	theme	wound-healing	1330:1342	arg1	genes					1344:1348	nine wound-healing genes	1325:1348	nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3)	1325:1407	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	29	theme	wound-healing	1330:1342	arg1	F3					1405:1406	F3	1405:1406	F3	1405:1406	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	29	theme	wound-healing	1330:1342	arg1	TGFB1					1388:1392	TGFB1	1388:1392	TGFB1	1388:1392	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	5	30	theme	water	910:914	arg1	rate					935:938	water vapor transmission rate	910:938	water vapor transmission rate	910:938	However, water vapor transmission rate, pore size, hydrophilicity (semidry), and topography were similar between all formulations, leading to a similar cell attachment and proliferation profile.					
30372014	2	31	theme	irradiation	436:446	arg1	doses					448:452	varying BNC to AA ratios and electron-beam irradiation doses	393:452	varying BNC to AA ratios and electron-beam irradiation doses	393:452	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	0	32	theme	Molecular	13:21	arg1	Interaction					23:33	Cellular and Molecular Interaction	0:33	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration	0:133	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	7	33	dep	genes	1344:1348	arg1	IL6					1351:1353	IL6	1351:1353	IL6	1351:1353	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	33	dep	genes	1344:1348	arg1	CTSK					1368:1371	CTSK	1368:1371	CTSK	1368:1371	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	33	dep	genes	1344:1348	arg1	FGF7					1374:1377	FGF7	1374:1377	FGF7	1374:1377	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	33	dep	genes	1344:1348	arg1	COX2					1395:1398	COX2	1395:1398	COX2	1395:1398	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	33	dep	genes	1344:1348	arg1	IL10					1356:1359	IL10	1356:1359	IL10	1356:1359	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	33	dep	genes	1344:1348	arg1	MMP2					1362:1365	MMP2	1362:1365	MMP2	1362:1365	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	33	dep	genes	1344:1348	arg1	GM-CSF					1380:1385	GM-CSF	1380:1385	GM-CSF	1380:1385	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	33	dep	genes	1344:1348	arg1	genes					1344:1348	nine wound-healing genes	1325:1348	nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3)	1325:1407	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	33	dep	genes	1344:1348	arg1	F3					1405:1406	F3	1405:1406	F3	1405:1406	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	7	33	dep	genes	1344:1348	arg1	TGFB1					1388:1392	TGFB1	1388:1392	TGFB1	1388:1392	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	3	34	theme	human	534:538	arg1	HDFs					560:563	HDFs	560:563	HDFs	560:563	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	3	34	theme	human	534:538	arg1	fibroblasts					547:557	human dermal fibroblasts	534:557	human dermal fibroblasts (HDFs)	534:564	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	2	35	theme	electron-beam	422:434	arg1	irradiation					436:446	electron-beam irradiation	422:446	electron-beam irradiation	422:446	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	0	36	theme	Human	38:42	arg1	Fibroblasts					51:61	Human Dermal Fibroblasts	38:61	Human Dermal Fibroblasts	38:61	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	6	37	theme	cells	1272:1276	arg1	viability					1182:1190	viability	1182:1190	viability	1182:1190	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	37	theme	cells	1272:1276	arg1	morphology					1196:1205	morphology	1196:1205	morphology	1196:1205	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	37	theme	cells	1272:1276	arg1	adhesion					1156:1163	rapid cell adhesion	1145:1163	rapid cell adhesion	1145:1163	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	37	theme	cells	1272:1276	arg1	transfer					1260:1267	facilitated fast transfer	1243:1267	facilitated fast transfer of cells	1243:1276	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	37	theme	cells	1272:1276	arg1	HDFs					1177:1180	maintained HDFs	1166:1180	maintained HDFs viability and morphology	1166:1205	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	37	theme	cells	1272:1276	arg1	migration					1228:1236	restricted cellular migration	1208:1236	restricted cellular migration	1208:1236	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	38	dep	HDFs	1177:1180	arg1	morphology					1196:1205	morphology	1196:1205	morphology	1196:1205	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	38	dep	HDFs	1177:1180	arg1	viability					1182:1190	viability	1182:1190	viability	1182:1190	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	38	dep	HDFs	1177:1180	arg1	HDFs					1177:1180	maintained HDFs	1166:1180	maintained HDFs viability and morphology	1166:1205	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	39	theme	maintained	1166:1175	arg1	morphology					1196:1205	morphology	1196:1205	morphology	1196:1205	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	39	theme	maintained	1166:1175	arg1	viability					1182:1190	viability	1182:1190	viability	1182:1190	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	39	theme	maintained	1166:1175	arg1	HDFs					1177:1180	maintained HDFs	1166:1180	maintained HDFs viability and morphology	1166:1205	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	0	40	with	Interaction	23:33	arg1	Hydrogel					102:109	Bacterial Nanocellulose Composite Hydrogel	68:109	Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration	68:133	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	0	41	theme	Fibroblasts	51:61	arg1	Interaction					23:33	Cellular and Molecular Interaction	0:33	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration	0:133	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	8	42	theme	BNC/AA	1441:1446	arg1	hydrogel					1448:1455	the BNC/AA hydrogel	1437:1455	the BNC/AA hydrogel	1437:1455	The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.					
30372014	8	42	theme	BNC/AA	1441:1446	arg1	biomaterial					1472:1482	a potential biomaterial	1460:1482	a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing	1460:1590	The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.					
30372014	4	43	theme	hydrogels	793:801	arg1	dry					781:783	dry	781:783	dry	781:783	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	43	theme	hydrogels	793:801	arg1	hardness					719:726	hardness	719:726	hardness	719:726	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	43	theme	hydrogels	793:801	arg1	porosity					751:758	porosity	751:758	porosity	751:758	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	43	theme	hydrogels	793:801	arg1	force					738:742	adhesive force	729:742	adhesive force (wet)	729:748	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	43	theme	hydrogels	793:801	arg1	wet					745:747	wet	745:747	wet	745:747	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	43	theme	hydrogels	793:801	arg1	hydrophilicity					765:778	hydrophilicity	765:778	hydrophilicity	765:778	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	43	theme	hydrogels	793:801	arg1	Swelling					709:716	Swelling	709:716	Swelling	709:716	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	6	44	theme	facilitated	1243:1253	arg1	transfer					1260:1267	facilitated fast transfer	1243:1267	facilitated fast transfer of cells	1243:1276	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	2	45	theme	BNC/AA	359:364	arg1	hydrogels					367:375	bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels	321:375	bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses	321:452	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	0	46	theme	Dermal	44:49	arg1	Fibroblasts					51:61	Human Dermal Fibroblasts	38:61	Human Dermal Fibroblasts	38:61	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	6	47	theme	restricted	1208:1217	arg1	migration					1228:1236	restricted cellular migration	1208:1236	restricted cellular migration	1208:1236	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	6	48	theme	fast	1255:1258	arg1	transfer					1260:1267	facilitated fast transfer	1243:1267	facilitated fast transfer of cells	1243:1276	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	4	49	theme	AA	867:868	arg1	AA					867:868	AA	867:868	AA	867:868	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	49	theme	AA	867:868	arg1	cross-linking					835:847	cross-linking	835:847	cross-linking	835:847	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	49	theme	AA	867:868	arg1	amount					857:862	the amount	853:862	the amount of AA incorporated in the hydrogels	853:898	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	49	theme	AA	867:868	arg1	degree					825:830	the degree	821:830	the degree of cross-linking	821:847	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	6	50	theme	cell	1151:1154	arg1	adhesion					1156:1163	rapid cell adhesion	1145:1163	rapid cell adhesion	1145:1163	At the cellular level, the hydrogel demonstrated rapid cell adhesion, maintained HDFs viability and morphology, restricted cellular migration, and facilitated fast transfer of cells.					
30372014	5	51	theme	pore	941:944	arg1	size					946:949	pore size	941:949	pore size	941:949	However, water vapor transmission rate, pore size, hydrophilicity (semidry), and topography were similar between all formulations, leading to a similar cell attachment and proliferation profile.					
30372014	2	52	theme	acid	353:356	arg1	hydrogels					367:375	bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels	321:375	bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses	321:452	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	8	53	theme	wound-dressing	1510:1523	arg1	material					1525:1532	a wound-dressing material	1508:1532	a wound-dressing material	1508:1532	The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.					
30372014	8	53	theme	wound-dressing	1510:1523	arg1	biomaterial					1472:1482	a potential biomaterial	1460:1482	a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing	1460:1590	The findings indicate that the BNC/AA hydrogel is a potential biomaterial that can be employed as a wound-dressing material to incorporate HDFs for the acceleration of wound healing.					
30372014	3	54	dep	manner	475:480	arg1	influence					508:516	influence	508:516	influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels	508:601	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	2	55	theme	nanocellulose/acrylic	331:351	arg1	hydrogels					367:375	bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels	321:375	bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses	321:452	In this study, the properties of bacterial nanocellulose/acrylic acid (BNC/AA) hydrogels fabricated with varying BNC to AA ratios and electron-beam irradiation doses were determined.					
30372014	4	56	theme	cross-linking	835:847	arg1	AA					867:868	AA	867:868	AA	867:868	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	56	theme	cross-linking	835:847	arg1	cross-linking					835:847	cross-linking	835:847	cross-linking	835:847	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	56	theme	cross-linking	835:847	arg1	amount					857:862	the amount	853:862	the amount of AA incorporated in the hydrogels	853:898	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	4	56	theme	cross-linking	835:847	arg1	degree					825:830	the degree	821:830	the degree of cross-linking	821:847	Swelling, hardness, adhesive force (wet), porosity, and hydrophilicity (dry) of the hydrogels were dependent on the degree of cross-linking and the amount of AA incorporated in the hydrogels.					
30372014	3	57	dep	application	645:655	arg1	both					657:660	both	657:660	both	657:660	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	0	58	theme	Bacterial	68:76	arg1	Hydrogel					102:109	Bacterial Nanocellulose Composite Hydrogel	68:109	Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration	68:133	Cellular and Molecular Interaction of Human Dermal Fibroblasts with Bacterial Nanocellulose Composite Hydrogel for Tissue Regeneration.					
30372014	3	59	theme	cell	667:670	arg1	carrier					672:678	a cell carrier	665:678	a cell carrier	665:678	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	5	60	theme	proliferation	1073:1085	arg1	profile					1087:1093	proliferation profile	1073:1093	proliferation profile	1073:1093	However, water vapor transmission rate, pore size, hydrophilicity (semidry), and topography were similar between all formulations, leading to a similar cell attachment and proliferation profile.					
30372014	3	61	theme	dermal	540:545	arg1	HDFs					560:563	HDFs	560:563	HDFs	560:563	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	3	61	theme	dermal	540:545	arg1	fibroblasts					547:557	human dermal fibroblasts	534:557	human dermal fibroblasts (HDFs)	534:564	The manner these hydrogel properties influence the behavior of human dermal fibroblasts (HDFs) at the cellular and molecular levels was also investigated, relating it to its application both as a cell carrier and wound dressing material.					
30372014	1	62	theme	biomaterial	248:258	arg1	suitability					229:239	the suitability	225:239	the suitability of the biomaterial for a specific application	225:285	The evaluation of the interaction of cells with biomaterials is fundamental to establish the suitability of the biomaterial for a specific application.					
30372014	5	63	theme	cell	1053:1056	arg1	attachment					1058:1067	a similar cell attachment	1043:1067	a similar cell attachment	1043:1067	However, water vapor transmission rate, pore size, hydrophilicity (semidry), and topography were similar between all formulations, leading to a similar cell attachment and proliferation profile.					
30372014	7	64	theme	molecular	1286:1294	arg1	level					1296:1300	the molecular level	1282:1300	the molecular level	1282:1300	At the molecular level, the hydrogel affected nine wound-healing genes (IL6, IL10, MMP2, CTSK, FGF7, GM-CSF, TGFB1, COX2, and F3).					
30372014	1	65	theme	interaction	158:168	arg1	evaluation					140:149	The evaluation	136:149	The evaluation of the interaction of cells with biomaterials	136:195	The evaluation of the interaction of cells with biomaterials is fundamental to establish the suitability of the biomaterial for a specific application.					
30372014	1	65	theme	interaction	158:168	arg1	fundamental					200:210	fundamental	200:210	fundamental	200:210	The evaluation of the interaction of cells with biomaterials is fundamental to establish the suitability of the biomaterial for a specific application.					
29729598	7	0	theme	adsorption	1005:1014	arg1	capacities					1016:1025	the obtained maximum adsorption capacities	984:1025	the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+	984:1073	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	7	0	theme	adsorption	1005:1014	arg1	247.16					1080:1085	247.16	1080:1085	247.16	1080:1085	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	3	1	theme	aqueous	550:556	arg1	solution					558:565	aqueous solution	550:565	aqueous solution	550:565	The SA/PVA/GO (SPG) hydrogels were employed as an efficient adsorbent for removal of Cu (II) and U (VI) from aqueous solution.					
29729598	7	2	theme	Langmuir	965:972	arg1	model					974:978	Langmuir model	965:978	Langmuir model	965:978	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	1	3	theme	/graphene	182:190	arg1	oxide					192:196	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide	130:196	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	2	4	theme	significant	392:402	arg1	role					404:407	a significant role	390:407	a significant role	390:407	Sodium alginate was physically crosslinked by Ca2+; GO was encapsulated into the composite to strengthen the hydrogels; PVA played a significant role in well dispersing of GO in SA.					
29729598	10	5	theme	U	1493:1493	arg1	removal					1470:1476	removal	1470:1476	removal of Cu (II) and U (VI) in wastewater	1470:1512	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	1	6	theme	oxide	192:196	arg1	microspheres					212:223	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	6	7	theme	order	860:864	arg1	model					866:870	pseudo-second order model	846:870	pseudo-second order model	846:870	Furthermore, the sorption kinetics of Cu2+ and UO22+ followed pseudo-second order model and exhibited 3-stage intraparticle diffusion model.					
29729598	10	8	attach	removal	1470:1476	arg3	wastewater					1503:1512	wastewater	1503:1512	wastewater	1503:1512	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	10	8	attach	removal	1470:1476	arg2	Cu					1481:1482	Cu (II)	1481:1487	Cu (II)	1481:1487	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	10	8	attach	removal	1470:1476	arg2	U					1493:1493	U	1493:1493	U (VI)	1493:1498	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	10	9	dep	U	1493:1493	arg1	VI					1496:1497	VI	1496:1497	VI	1496:1497	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	8	10	theme	elements	1247:1254	arg1	peak					1239:1242	peak	1239:1242	peak of elements in XPS spectra	1239:1269	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	4	11	theme	pH	610:611	arg1	subject					595:601	the subject	591:601	the subject of the pH, initial metal ion concentration, competing ions and contact time	591:677	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	8	12	from	spectra	1210:1216	arg1	intension					1226:1234	the intension	1222:1234	the intension of peak of elements in XPS spectra	1222:1269	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	8	12	from	spectra	1210:1216	arg1	percentage					1180:1189	the percentage	1176:1189	the percentage of elements in EDX spectra	1176:1216	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	3	13	dep	U	538:538	arg1	VI					541:542	VI	541:542	VI	541:542	The SA/PVA/GO (SPG) hydrogels were employed as an efficient adsorbent for removal of Cu (II) and U (VI) from aqueous solution.					
29729598	7	14	theme	microspheres	1043:1054	arg1	capacities					1016:1025	the obtained maximum adsorption capacities	984:1025	the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+	984:1073	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	7	14	theme	microspheres	1043:1054	arg1	247.16					1080:1085	247.16	1080:1085	247.16	1080:1085	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	9	15	theme	excellent	1298:1306	arg1	reusability					1308:1318	excellent reusability	1298:1318	excellent reusability	1298:1318	The SPG sorbent exhibited excellent reusability after 5 adsorption-desorption cycles.					
29729598	7	16	theme	obtained	988:995	arg1	capacities					1016:1025	the obtained maximum adsorption capacities	984:1025	the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+	984:1073	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	7	16	theme	obtained	988:995	arg1	247.16					1080:1085	247.16	1080:1085	247.16	1080:1085	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	3	17	theme	SA/PVA/GO	445:453	arg1	adsorbent					501:509	adsorbent	501:509	adsorbent	501:509	The SA/PVA/GO (SPG) hydrogels were employed as an efficient adsorbent for removal of Cu (II) and U (VI) from aqueous solution.					
29729598	3	17	theme	SA/PVA/GO	445:453	arg1	hydrogels					461:469	The SA/PVA/GO (SPG) hydrogels	441:469	The SA/PVA/GO (SPG) hydrogels	441:469	The SA/PVA/GO (SPG) hydrogels were employed as an efficient adsorbent for removal of Cu (II) and U (VI) from aqueous solution.					
29729598	8	18	from	elements	1247:1254	arg1	spectra					1263:1269	XPS spectra	1259:1269	XPS spectra	1259:1269	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	7	19	theme	hydrogel	1034:1041	arg1	microspheres					1043:1054	SPG hydrogel microspheres	1030:1054	SPG hydrogel microspheres for Cu2+ and UO22+	1030:1073	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	6	20	theme	exhibited	876:884	arg1	model					918:922	exhibited 3-stage intraparticle diffusion model	876:922	exhibited 3-stage intraparticle diffusion model	876:922	Furthermore, the sorption kinetics of Cu2+ and UO22+ followed pseudo-second order model and exhibited 3-stage intraparticle diffusion model.					
29729598	4	21	theme	initial	614:620	arg1	concentration					632:644	initial metal ion concentration	614:644	initial metal ion concentration	614:644	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	6	22	theme	diffusion	908:916	arg1	model					918:922	exhibited 3-stage intraparticle diffusion model	876:922	exhibited 3-stage intraparticle diffusion model	876:922	Furthermore, the sorption kinetics of Cu2+ and UO22+ followed pseudo-second order model and exhibited 3-stage intraparticle diffusion model.					
29729598	5	23	theme	Structure	698:706	arg1	characterization					708:723	Structure characterization	698:723	Structure characterization	698:723	Structure characterization was successfully conducted by FTIR, SEM, EDX, BET and XPS.					
29729598	0	24	theme	U	100:100	arg1	removal					107:113	U (VI) removal	100:113	U (VI) removal	100:113	Graphene oxide encapsulated polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal.					
29729598	4	25	theme	time	674:677	arg1	subject					595:601	the subject	591:601	the subject of the pH, initial metal ion concentration, competing ions and contact time	591:677	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	8	26	from	percentage	1180:1189	arg1	spectra					1210:1216	EDX spectra	1206:1216	EDX spectra	1206:1216	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	7	27	theme	SPG	1030:1032	arg1	microspheres					1043:1054	SPG hydrogel microspheres	1030:1054	SPG hydrogel microspheres for Cu2+ and UO22+	1030:1073	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	8	28	from	peak	1239:1242	arg1	spectra					1263:1269	XPS spectra	1259:1269	XPS spectra	1259:1269	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	1	29	theme	novel	132:136	arg1	PVA					178:180	PVA	178:180	PVA	178:180	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	1	29	theme	novel	132:136	arg1	alcohol					169:175	novel sodium alginate (SA)/polyvinyl alcohol	132:175	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	3	30	from	solution	558:565	arg1	removal					515:521	removal	515:521	removal of Cu (II) and U (VI) from aqueous solution	515:565	The SA/PVA/GO (SPG) hydrogels were employed as an efficient adsorbent for removal of Cu (II) and U (VI) from aqueous solution.					
29729598	10	31	theme	prepared	1389:1396	arg1	adsorbents					1398:1407	the prepared adsorbents	1385:1407	the prepared adsorbents	1385:1407	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	10	31	theme	prepared	1389:1396	arg1	materials					1456:1464	effective and promising materials	1432:1464	effective and promising materials	1432:1464	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	7	32	theme	Equilibrium	925:935	arg1	data					937:940	Equilibrium data	925:940	Equilibrium data	925:940	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	8	33	from	spectra	1263:1269	arg1	peak					1239:1242	peak	1239:1242	peak of elements in XPS spectra	1239:1269	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	1	34	theme	sodium	138:143	arg1	PVA					178:180	PVA	178:180	PVA	178:180	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	1	34	theme	sodium	138:143	arg1	alcohol					169:175	novel sodium alginate (SA)/polyvinyl alcohol	132:175	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	0	35	theme	Graphene	0:7	arg1	oxide					9:13	Graphene oxide	0:13	Graphene oxide	0:13	Graphene oxide encapsulated polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal.					
29729598	6	36	theme	sorption	801:808	arg1	kinetics					810:817	the sorption kinetics	797:817	the sorption kinetics of Cu2+ and UO22+	797:835	Furthermore, the sorption kinetics of Cu2+ and UO22+ followed pseudo-second order model and exhibited 3-stage intraparticle diffusion model.					
29729598	1	37	theme	alginate	145:152	arg1	PVA					178:180	PVA	178:180	PVA	178:180	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	1	37	theme	alginate	145:152	arg1	alcohol					169:175	novel sodium alginate (SA)/polyvinyl alcohol	132:175	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	0	38	theme	polyvinyl	28:36	arg1	microspheres					71:82	polyvinyl alcohol/sodium alginate hydrogel microspheres	28:82	polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal	28:113	Graphene oxide encapsulated polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal.					
29729598	1	39	theme	hydrogel	203:210	arg1	microspheres					212:223	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	8	40	theme	peak	1239:1242	arg1	intension					1226:1234	the intension	1222:1234	the intension of peak of elements in XPS spectra	1222:1269	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	8	40	theme	peak	1239:1242	arg1	percentage					1180:1189	the percentage	1176:1189	the percentage of elements in EDX spectra	1176:1216	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	6	41	theme	3-stage	886:892	arg1	model					918:922	exhibited 3-stage intraparticle diffusion model	876:922	exhibited 3-stage intraparticle diffusion model	876:922	Furthermore, the sorption kinetics of Cu2+ and UO22+ followed pseudo-second order model and exhibited 3-stage intraparticle diffusion model.					
29729598	10	42	theme	promising	1446:1454	arg1	materials					1456:1464	effective and promising materials	1432:1464	effective and promising materials	1432:1464	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	10	42	theme	promising	1446:1454	arg1	adsorbents					1398:1407	the prepared adsorbents	1385:1407	the prepared adsorbents	1385:1407	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	8	43	theme	EDX	1206:1208	arg1	spectra					1210:1216	EDX spectra	1206:1216	EDX spectra	1206:1216	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	3	44	theme	SPG	456:458	arg1	adsorbent					501:509	adsorbent	501:509	adsorbent	501:509	The SA/PVA/GO (SPG) hydrogels were employed as an efficient adsorbent for removal of Cu (II) and U (VI) from aqueous solution.					
29729598	3	44	theme	SPG	456:458	arg1	hydrogels					461:469	The SA/PVA/GO (SPG) hydrogels	441:469	The SA/PVA/GO (SPG) hydrogels	441:469	The SA/PVA/GO (SPG) hydrogels were employed as an efficient adsorbent for removal of Cu (II) and U (VI) from aqueous solution.					
29729598	7	45	theme	maximum	997:1003	arg1	capacities					1016:1025	the obtained maximum adsorption capacities	984:1025	the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+	984:1073	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	7	45	theme	maximum	997:1003	arg1	247.16					1080:1085	247.16	1080:1085	247.16	1080:1085	Equilibrium data were best described by Langmuir model and the obtained maximum adsorption capacities of SPG hydrogel microspheres for Cu2+ and UO22+ were 247.16 and 403.78 mg/g, respectively.					
29729598	1	46	theme	SA	155:156	arg1	PVA					178:180	PVA	178:180	PVA	178:180	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	1	46	theme	SA	155:156	arg1	alcohol					169:175	novel sodium alginate (SA)/polyvinyl alcohol	132:175	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	0	47	theme	alginate	53:60	arg1	microspheres					71:82	polyvinyl alcohol/sodium alginate hydrogel microspheres	28:82	polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal	28:113	Graphene oxide encapsulated polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal.					
29729598	8	48	theme	adsorption	1136:1145	arg1	capacity					1147:1154	adsorption capacity	1136:1154	adsorption capacity	1136:1154	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	10	49	theme	effective	1432:1440	arg1	materials					1456:1464	effective and promising materials	1432:1464	effective and promising materials	1432:1464	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	10	49	theme	effective	1432:1440	arg1	adsorbents					1398:1407	the prepared adsorbents	1385:1407	the prepared adsorbents	1385:1407	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	4	50	theme	ions	657:660	arg1	subject					595:601	the subject	591:601	the subject of the pH, initial metal ion concentration, competing ions and contact time	591:677	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	6	51	theme	intraparticle	894:906	arg1	model					918:922	exhibited 3-stage intraparticle diffusion model	876:922	exhibited 3-stage intraparticle diffusion model	876:922	Furthermore, the sorption kinetics of Cu2+ and UO22+ followed pseudo-second order model and exhibited 3-stage intraparticle diffusion model.					
29729598	0	52	theme	alcohol/sodium	38:51	arg1	microspheres					71:82	polyvinyl alcohol/sodium alginate hydrogel microspheres	28:82	polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal	28:113	Graphene oxide encapsulated polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal.					
29729598	6	53	theme	Cu2+	822:825	arg1	kinetics					810:817	the sorption kinetics	797:817	the sorption kinetics of Cu2+ and UO22+	797:835	Furthermore, the sorption kinetics of Cu2+ and UO22+ followed pseudo-second order model and exhibited 3-stage intraparticle diffusion model.					
29729598	4	54	theme	competing	647:655	arg1	ions					657:660	competing ions	647:660	competing ions	647:660	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	1	55	theme	/polyvinyl	158:167	arg1	PVA					178:180	PVA	178:180	PVA	178:180	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	1	55	theme	/polyvinyl	158:167	arg1	alcohol					169:175	novel sodium alginate (SA)/polyvinyl alcohol	132:175	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	10	56	theme	Cu	1481:1482	arg1	removal					1470:1476	removal	1470:1476	removal of Cu (II) and U (VI) in wastewater	1470:1512	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	8	57	theme	elements	1194:1201	arg1	intension					1226:1234	the intension	1222:1234	the intension of peak of elements in XPS spectra	1222:1269	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	8	57	theme	elements	1194:1201	arg1	percentage					1180:1189	the percentage	1176:1189	the percentage of elements in EDX spectra	1176:1216	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	3	58	theme	Cu	526:527	arg1	removal					515:521	removal	515:521	removal of Cu (II) and U (VI) from aqueous solution	515:565	The SA/PVA/GO (SPG) hydrogels were employed as an efficient adsorbent for removal of Cu (II) and U (VI) from aqueous solution.					
29729598	4	59	theme	contact	666:672	arg1	time					674:677	contact time	666:677	contact time	666:677	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	9	60	theme	adsorption-desorption	1328:1348	arg1	cycles					1350:1355	5 adsorption-desorption cycles	1326:1355	5 adsorption-desorption cycles	1326:1355	The SPG sorbent exhibited excellent reusability after 5 adsorption-desorption cycles.					
29729598	0	61	theme	hydrogel	62:69	arg1	microspheres					71:82	polyvinyl alcohol/sodium alginate hydrogel microspheres	28:82	polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal	28:113	Graphene oxide encapsulated polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal.					
29729598	3	62	theme	U	538:538	arg1	removal					515:521	removal	515:521	removal of Cu (II) and U (VI) from aqueous solution	515:565	The SA/PVA/GO (SPG) hydrogels were employed as an efficient adsorbent for removal of Cu (II) and U (VI) from aqueous solution.					
29729598	2	63	theme	Sodium	259:264	arg1	alginate					266:273	Sodium alginate	259:273	Sodium alginate	259:273	Sodium alginate was physically crosslinked by Ca2+; GO was encapsulated into the composite to strengthen the hydrogels; PVA played a significant role in well dispersing of GO in SA.					
29729598	1	64	theme	simple	244:249	arg1	method					251:256	a simple method	242:256	a simple method	242:256	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	8	65	theme	XPS	1259:1261	arg1	spectra					1263:1269	XPS spectra	1259:1269	XPS spectra	1259:1269	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	4	66	theme	ion	628:630	arg1	concentration					632:644	initial metal ion concentration	614:644	initial metal ion concentration	614:644	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	1	67	theme	alcohol	169:175	arg1	oxide					192:196	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide	130:196	a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres	130:223	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	4	68	theme	metal	622:626	arg1	concentration					632:644	initial metal ion concentration	614:644	initial metal ion concentration	614:644	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	10	69	from	removal	1470:1476	arg1	wastewater					1503:1512	wastewater	1503:1512	wastewater	1503:1512	All results suggested that the prepared adsorbents could be considered as effective and promising materials for removal of Cu (II) and U (VI) in wastewater.					
29729598	4	70	theme	Batch	568:572	arg1	experiments					574:584	Batch experiments	568:584	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time	568:677	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	8	71	from	elements	1194:1201	arg1	spectra					1210:1216	EDX spectra	1206:1216	EDX spectra	1206:1216	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	6	72	theme	pseudo-second	846:858	arg1	model					866:870	pseudo-second order model	846:870	pseudo-second order model	846:870	Furthermore, the sorption kinetics of Cu2+ and UO22+ followed pseudo-second order model and exhibited 3-stage intraparticle diffusion model.					
29729598	1	73	dep	oxide	192:196	arg1	GO					199:200	GO	199:200	GO	199:200	In this work, a novel sodium alginate (SA)/polyvinyl alcohol (PVA)/graphene oxide (GO) hydrogel microspheres were prepared by a simple method.					
29729598	8	74	from	difference	1122:1131	arg1	capacity					1147:1154	adsorption capacity	1136:1154	adsorption capacity	1136:1154	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	9	75	theme	SPG	1276:1278	arg1	sorbent					1280:1286	The SPG sorbent	1272:1286	The SPG sorbent	1272:1286	The SPG sorbent exhibited excellent reusability after 5 adsorption-desorption cycles.					
29729598	4	76	with	experiments	574:584	arg1	subject					595:601	the subject	591:601	the subject of the pH, initial metal ion concentration, competing ions and contact time	591:677	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	4	77	theme	concentration	632:644	arg1	subject					595:601	the subject	591:601	the subject of the pH, initial metal ion concentration, competing ions and contact time	591:677	Batch experiments with the subject of the pH, initial metal ion concentration, competing ions and contact time were investigated.					
29729598	0	78	dep	removal	107:113	arg1	VI					103:104	VI	103:104	VI	103:104	Graphene oxide encapsulated polyvinyl alcohol/sodium alginate hydrogel microspheres for Cu (II) and U (VI) removal.					
29729598	8	79	from	intension	1226:1234	arg1	spectra					1210:1216	EDX spectra	1206:1216	EDX spectra	1206:1216	The difference in adsorption capacity can be confirmed by the percentage of elements in EDX spectra and the intension of peak of elements in XPS spectra.					
29729598	6	80	theme	UO22+	831:835	arg1	kinetics					810:817	the sorption kinetics	797:817	the sorption kinetics of Cu2+ and UO22+	797:835	Furthermore, the sorption kinetics of Cu2+ and UO22+ followed pseudo-second order model and exhibited 3-stage intraparticle diffusion model.					
31733847	7	0	from	±	1286:1286	arg1	4.00					1275:1278	4.00	1275:1278	4.00	1275:1278	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	13	1	theme	Simmental	2383:2391	arg1	quality					2356:2362	milk quality	2351:2362	milk quality of Brown Swiss and Simmental cows	2351:2396	The best milk yield and quality were observed between SCS 0.00 and 1.00, but milk quality of Holstein Friesians started to decrease at lower SCS compared with milk quality of Brown Swiss and Simmental cows.					
31733847	4	2	theme	multibreed	891:900	arg1	herds					902:906	313 multibreed herds	887:906	313 multibreed herds	887:906	The data set comprised 95,591 observations of 6,940 cows in 313 multibreed herds, collected from January 2011 to December 2017.					
31733847	2	3	theme	multibreed	721:730	arg1	herds					732:736	multibreed herds	721:736	multibreed herds	721:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	0	4	theme	cattle	157:162	arg1	breeds					164:169	cattle breeds	157:169	cattle breeds	157:169	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	1	5	theme	coagulation	365:375	arg1	properties					377:386	coagulation properties	365:386	coagulation properties	365:386	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	14	6	theme	mastitis	2574:2581	arg1	detection					2561:2569	early detection	2555:2569	early detection of mastitis	2555:2581	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	11	7	theme	±	1961:1961	arg1	d					1965:1965	the following 90 ± 5 d	1944:1965	the following 90 ± 5 d	1944:1965	Moreover, cows with SCS lower than -1.00 on a test day were about 7 times more likely to present high SCS within the following 90 ± 5 d than cows with SCS between -1.00 and 0.00.					
31733847	9	8	theme	milk	1583:1586	arg1	loss					1575:1578	The average loss	1563:1578	The average loss of milk, fat, and crude protein yields	1563:1617	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	9	8	theme	milk	1583:1586	arg1	kg/d					1645:1648	0.43, 0.01, and 0.01 kg/d	1624:1648	0.43, 0.01, and 0.01 kg/d	1624:1648	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	2	9	theme	cows	711:714	arg1	composition					523:533	composition	523:533	composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents)	523:632	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	9	theme	cows	711:714	arg1	yield					516:520	milk yield	511:520	milk yield	511:520	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	9	theme	cows	711:714	arg1	properties					651:660	coagulation properties	639:660	coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds	639:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	13	10	theme	best	2196:2199	arg1	yield					2206:2210	The best milk yield	2192:2210	The best milk yield	2192:2210	The best milk yield and quality were observed between SCS 0.00 and 1.00, but milk quality of Holstein Friesians started to decrease at lower SCS compared with milk quality of Brown Swiss and Simmental cows.					
31733847	2	11	theme	milk	511:514	arg1	yield					516:520	milk yield	511:520	milk yield	511:520	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	6	12	theme	Statistical	1010:1020	arg1	analysis					1022:1029	Statistical analysis	1010:1029	Statistical analysis	1010:1029	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	9	13	theme	fat	1589:1591	arg1	loss					1575:1578	The average loss	1563:1578	The average loss of milk, fat, and crude protein yields	1563:1617	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	9	13	theme	fat	1589:1591	arg1	kg/d					1645:1648	0.43, 0.01, and 0.01 kg/d	1624:1648	0.43, 0.01, and 0.01 kg/d	1624:1648	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	13	14	theme	Swiss	2373:2377	arg1	quality					2356:2362	milk quality	2351:2362	milk quality of Brown Swiss and Simmental cows	2351:2396	The best milk yield and quality were observed between SCS 0.00 and 1.00, but milk quality of Holstein Friesians started to decrease at lower SCS compared with milk quality of Brown Swiss and Simmental cows.					
31733847	1	15	theme	High	172:175	arg1	SCC					202:204	SCC	202:204	SCC	202:204	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	1	15	theme	High	172:175	arg1	count					195:199	High milk somatic cell count	172:199	High milk somatic cell count (SCC)	172:205	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	4	16	dep	January	924:930	arg1	December					940:947	December	940:947	December	940:947	The data set comprised 95,591 observations of 6,940 cows in 313 multibreed herds, collected from January 2011 to December 2017.					
31733847	4	16	dep	January	924:930	arg1	2011					932:935	2011	932:935	January 2011 to December 2017	924:952	The data set comprised 95,591 observations of 6,940 cows in 313 multibreed herds, collected from January 2011 to December 2017.					
31733847	4	16	dep	January	924:930	arg1	to					937:938	to	937:938	to	937:938	The data set comprised 95,591 observations of 6,940 cows in 313 multibreed herds, collected from January 2011 to December 2017.					
31733847	11	17	with	cows	1841:1844	arg1	SCS					1851:1853	SCS lower than -1.00	1851:1870	SCS lower than -1.00	1851:1870	Moreover, cows with SCS lower than -1.00 on a test day were about 7 times more likely to present high SCS within the following 90 ± 5 d than cows with SCS between -1.00 and 0.00.					
31733847	9	18	theme	protein	1604:1610	arg1	yields					1612:1617	crude protein yields	1598:1617	crude protein yields	1598:1617	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	1	19	theme	somatic	182:188	arg1	SCC					202:204	SCC	202:204	SCC	202:204	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	1	19	theme	somatic	182:188	arg1	count					195:199	High milk somatic cell count	172:199	High milk somatic cell count (SCC)	172:205	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	10	20	theme	milk	1750:1753	arg1	yield					1755:1759	milk yield	1750:1759	milk yield	1750:1759	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	6	21	theme	random	1215:1220	arg1	effects					1222:1228	residual as random effects	1203:1228	residual as random effects	1203:1228	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	0	22	theme	Simmental	147:155	arg1	composition					71:81	composition	71:81	composition	71:81	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	0	22	theme	Simmental	147:155	arg1	traits					101:106	technological traits	87:106	technological traits	87:106	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	12	23	theme	crude	2093:2097	arg1	yields					2107:2112	crude protein yields	2093:2112	crude protein yields	2093:2112	Breeds responded similarly to the increase of SCS, but the overall loss of fat and crude protein yields, and several minerals among Holstein Friesian were lower with increasing SCS.					
31733847	0	24	theme	Friesian	133:140	arg1	composition					71:81	composition	71:81	composition	71:81	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	0	24	theme	Friesian	133:140	arg1	traits					101:106	technological traits	87:106	technological traits	87:106	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	7	25	theme	test	1333:1336	arg1	day					1338:1340	the observation test day	1317:1340	the observation test day	1317:1340	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	2	26	from	effects	438:444	arg1	composition					523:533	composition	523:533	composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents)	523:632	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	26	from	effects	438:444	arg1	yield					516:520	milk yield	511:520	milk yield	511:520	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	26	from	effects	438:444	arg1	properties					651:660	coagulation properties	639:660	coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds	639:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	6	27	theme	linear	1053:1058	arg1	model					1066:1070	a linear mixed model	1051:1070	a linear mixed model	1051:1070	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	1	28	theme	content	353:359	arg1	terms					336:340	terms	336:340	terms of mineral content and coagulation properties	336:386	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	7	29	theme	SCS	1425:1427	arg1	≤					1429:1429	-1.00 < SCS ≤ 0.00	1417:1434	-1.00 < SCS ≤ 0.00	1417:1434	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	7	29	theme	SCS	1425:1427	arg1	class					1410:1414	the reference class	1396:1414	the reference class (-1.00 < SCS ≤ 0.00)	1396:1435	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	12	30	theme	SCS	2056:2058	arg1	increase					2044:2051	the increase	2040:2051	the increase of SCS	2040:2058	Breeds responded similarly to the increase of SCS, but the overall loss of fat and crude protein yields, and several minerals among Holstein Friesian were lower with increasing SCS.					
31733847	2	31	theme	urea	578:581	arg1	nitrogen					583:590	milk urea nitrogen	573:590	milk urea nitrogen	573:590	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	6	32	theme	as	1212:1213	arg1	effects					1222:1228	residual as random effects	1203:1228	residual as random effects	1203:1228	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	14	33	theme	SCS	2444:2446	arg1	optimum					2433:2439	a breed-dependent optimum	2415:2439	a breed-dependent optimum of SCS	2415:2446	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	14	34	theme	health	2512:2517	arg1	problems					2519:2526	udder health problems	2506:2526	udder health problems	2506:2526	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	2	35	theme	cell	457:460	arg1	SCS					469:471	SCS	469:471	SCS	469:471	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	35	theme	cell	457:460	arg1	score					462:466	somatic cell score	449:466	somatic cell score (SCS)	449:472	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	0	36	from	Effects	0:6	arg1	mid-infrared					48:59	mid-infrared	48:59	mid-infrared	48:59	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	0	36	from	Effects	0:6	arg1	yield					38:42	milk yield	33:42	milk yield	33:42	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	12	37	theme	protein	2099:2105	arg1	yields					2107:2112	crude protein yields	2093:2112	crude protein yields	2093:2112	Breeds responded similarly to the increase of SCS, but the overall loss of fat and crude protein yields, and several minerals among Holstein Friesian were lower with increasing SCS.					
31733847	1	38	theme	SCC	301:303	arg1	effect					287:292	the effect	283:292	the effect of low SCC on milk quality	283:319	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	8	39	theme	milk	1505:1508	arg1	traits					1510:1515	milk traits	1505:1515	milk traits	1505:1515	Results showed that the relationship between SCS and milk traits followed a third-order polynomial regression.					
31733847	3	40	theme	Milk	739:742	arg1	composition					744:754	Milk composition	739:754	Milk composition	739:754	Milk composition and coagulation traits were predicted using mid-infrared spectroscopy.					
31733847	11	41	with	cows	1972:1975	arg1	SCS					1982:1984	SCS	1982:1984	SCS between -1.00 and 0.00	1982:2007	Moreover, cows with SCS lower than -1.00 on a test day were about 7 times more likely to present high SCS within the following 90 ± 5 d than cows with SCS between -1.00 and 0.00.					
31733847	11	42	theme	high	1928:1931	arg1	SCS					1933:1935	present high SCS	1920:1935	present high SCS	1920:1935	Moreover, cows with SCS lower than -1.00 on a test day were about 7 times more likely to present high SCS within the following 90 ± 5 d than cows with SCS between -1.00 and 0.00.					
31733847	2	43	from	properties	651:660	arg1	herds					732:736	multibreed herds	721:736	multibreed herds	721:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	6	44	theme	fixed	1160:1164	arg1	effects					1166:1172	fixed effects	1160:1172	fixed effects	1160:1172	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	1	45	theme	milk	308:311	arg1	quality					313:319	milk quality	308:319	milk quality	308:319	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	4	46	theme	cows	879:882	arg1	observations					857:868	95,591 observations	850:868	95,591 observations of 6,940 cows in 313 multibreed herds, collected from January 2011 to December 2017	850:952	The data set comprised 95,591 observations of 6,940 cows in 313 multibreed herds, collected from January 2011 to December 2017.					
31733847	2	47	theme	crude	541:545	arg1	protein					547:553	crude protein	541:553	crude protein	541:553	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	8	48	theme	third-order	1528:1538	arg1	regression					1551:1560	a third-order polynomial regression	1526:1560	a third-order polynomial regression	1526:1560	Results showed that the relationship between SCS and milk traits followed a third-order polynomial regression.					
31733847	11	49	theme	following	1948:1956	arg1	d					1965:1965	the following 90 ± 5 d	1944:1965	the following 90 ± 5 d	1944:1965	Moreover, cows with SCS lower than -1.00 on a test day were about 7 times more likely to present high SCS within the following 90 ± 5 d than cows with SCS between -1.00 and 0.00.					
31733847	0	50	theme	somatic	11:17	arg1	score					24:28	somatic cell score	11:28	somatic cell score	11:28	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	14	51	theme	early	2555:2559	arg1	detection					2561:2569	early detection	2555:2569	early detection of mastitis	2555:2581	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	0	52	theme	score	24:28	arg1	Effects					0:6	Effects	0:6	Effects of somatic cell score on milk yield and mid-infrared	0:59	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	3	53	theme	mid-infrared	800:811	arg1	spectroscopy					813:824	mid-infrared spectroscopy	800:824	mid-infrared spectroscopy	800:824	Milk composition and coagulation traits were predicted using mid-infrared spectroscopy.					
31733847	11	54	from	cows	1841:1844	arg1	day					1882:1884	a test day	1875:1884	a test day	1875:1884	Moreover, cows with SCS lower than -1.00 on a test day were about 7 times more likely to present high SCS within the following 90 ± 5 d than cows with SCS between -1.00 and 0.00.					
31733847	0	55	theme	milk	33:36	arg1	yield					38:42	milk yield	33:42	milk yield	33:42	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	10	56	theme	low	1706:1708	arg1	<-1.00					1715:1720	<-1.00	1715:1720	<-1.00	1715:1720	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	10	56	theme	low	1706:1708	arg1	SCS					1710:1712	Very low SCS	1701:1712	Very low SCS (<-1.00)	1701:1721	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	12	57	theme	several	2119:2125	arg1	minerals					2127:2134	several minerals	2119:2134	several minerals among Holstein Friesian	2119:2158	Breeds responded similarly to the increase of SCS, but the overall loss of fat and crude protein yields, and several minerals among Holstein Friesian were lower with increasing SCS.					
31733847	10	58	theme	detrimental	1727:1737	arg1	effects					1739:1745	detrimental effects	1727:1745	detrimental effects on milk yield and quality traits	1727:1778	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	9	59	theme	average	1567:1573	arg1	loss					1575:1578	The average loss	1563:1578	The average loss of milk, fat, and crude protein yields	1563:1617	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	9	59	theme	average	1567:1573	arg1	kg/d					1645:1648	0.43, 0.01, and 0.01 kg/d	1624:1648	0.43, 0.01, and 0.01 kg/d	1624:1648	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	2	60	theme	Holstein	678:685	arg1	composition					523:533	composition	523:533	composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents)	523:632	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	60	theme	Holstein	678:685	arg1	yield					516:520	milk yield	511:520	milk yield	511:520	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	60	theme	Holstein	678:685	arg1	properties					651:660	coagulation properties	639:660	coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds	639:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	14	61	theme	problems	2519:2526	arg1	SCS					2479:2481	very low SCS	2470:2481	very low SCS	2470:2481	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	14	61	theme	problems	2519:2526	arg1	indicator					2493:2501	an indicator	2490:2501	an indicator of udder health problems	2490:2526	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	6	62	theme	lactation	1112:1120	arg1	class					1127:1131	SCS class	1123:1131	SCS class	1123:1131	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	6	62	theme	lactation	1112:1120	arg1	stage					1103:1107	stage	1103:1107	stage of lactation	1103:1120	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	6	62	theme	lactation	1112:1120	arg1	parity					1095:1100	parity	1095:1100	parity	1095:1100	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	6	62	theme	lactation	1112:1120	arg1	interactions					1144:1155	their interactions	1138:1155	their interactions as fixed effects, and herd test day, cow, and residual as random effects	1138:1228	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	6	62	theme	lactation	1112:1120	arg1	breed					1088:1092	breed	1088:1092	breed	1088:1092	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	1	63	theme	milk	218:221	arg1	production					223:232	milk production	218:232	milk production	218:232	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	2	64	from	herds	732:736	arg1	composition					523:533	composition	523:533	composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents)	523:632	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	64	from	herds	732:736	arg1	cows					711:714	Simmental cows	701:714	Simmental cows	701:714	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	64	from	herds	732:736	arg1	yield					516:520	milk yield	511:520	milk yield	511:520	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	64	from	herds	732:736	arg1	properties					651:660	coagulation properties	639:660	coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds	639:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	1	65	theme	properties	377:386	arg1	terms					336:340	terms	336:340	terms of mineral content and coagulation properties	336:386	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	2	66	theme	Simmental	701:709	arg1	cows					711:714	Simmental cows	701:714	Simmental cows	701:714	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	14	67	theme	breed-dependent	2417:2431	arg1	optimum					2433:2439	a breed-dependent optimum	2415:2439	a breed-dependent optimum of SCS	2415:2446	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	13	68	theme	milk	2201:2204	arg1	yield					2206:2210	The best milk yield	2192:2210	The best milk yield	2192:2210	The best milk yield and quality were observed between SCS 0.00 and 1.00, but milk quality of Holstein Friesians started to decrease at lower SCS compared with milk quality of Brown Swiss and Simmental cows.					
31733847	1	69	from	effect	287:292	arg1	quality					313:319	milk quality	308:319	milk quality	308:319	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	2	70	theme	protein	593:599	arg1	fractions					601:609	protein fractions	593:609	protein fractions	593:609	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	9	71	theme	crude	1598:1602	arg1	yields					1612:1617	crude protein yields	1598:1617	crude protein yields	1598:1617	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	0	72	theme	Swiss	117:121	arg1	composition					71:81	composition	71:81	composition	71:81	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	0	72	theme	Swiss	117:121	arg1	traits					101:106	technological traits	87:106	technological traits	87:106	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	14	73	theme	low	2475:2477	arg1	SCS					2479:2481	very low SCS	2470:2481	very low SCS	2470:2481	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	14	73	theme	low	2475:2477	arg1	indicator					2493:2501	an indicator	2490:2501	an indicator of udder health problems	2490:2526	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	1	74	theme	milk	177:180	arg1	SCC					202:204	SCC	202:204	SCC	202:204	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	1	74	theme	milk	177:180	arg1	count					195:199	High milk somatic cell count	172:199	High milk somatic cell count (SCC)	172:205	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	2	75	theme	mineral	616:622	arg1	contents					624:631	mineral contents	616:631	mineral contents	616:631	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	10	76	theme	quality	1765:1771	arg1	traits					1773:1778	milk yield and quality traits	1750:1778	milk yield and quality traits	1750:1778	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	1	77	theme	cell	190:193	arg1	SCC					202:204	SCC	202:204	SCC	202:204	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	1	77	theme	cell	190:193	arg1	count					195:199	High milk somatic cell count	172:199	High milk somatic cell count (SCC)	172:205	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	2	78	theme	score	462:466	arg1	effects					438:444	the effects	434:444	the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds	434:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	13	79	dep	Swiss	2373:2377	arg1	cows					2393:2396	cows	2393:2396	cows	2393:2396	The best milk yield and quality were observed between SCS 0.00 and 1.00, but milk quality of Holstein Friesians started to decrease at lower SCS compared with milk quality of Brown Swiss and Simmental cows.					
31733847	7	80	theme	SCS	1366:1368	arg1	class					1370:1374	each SCS class	1361:1374	each SCS class	1361:1374	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	7	81	theme	observation	1321:1331	arg1	day					1338:1340	the observation test day	1317:1340	the observation test day	1317:1340	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	6	82	theme	mixed	1060:1064	arg1	model					1066:1070	a linear mixed model	1051:1070	a linear mixed model	1051:1070	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	2	83	from	yield	516:520	arg1	herds					732:736	multibreed herds	721:736	multibreed herds	721:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	6	84	theme	SCS	1123:1125	arg1	class					1127:1131	SCS class	1123:1131	SCS class	1123:1131	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	1	85	theme	mineral	345:351	arg1	content					353:359	mineral content	345:359	mineral content	345:359	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	13	86	theme	milk	2351:2354	arg1	quality					2356:2362	milk quality	2351:2362	milk quality of Brown Swiss and Simmental cows	2351:2396	The best milk yield and quality were observed between SCS 0.00 and 1.00, but milk quality of Holstein Friesians started to decrease at lower SCS compared with milk quality of Brown Swiss and Simmental cows.					
31733847	10	87	theme	yield	1755:1759	arg1	traits					1773:1778	milk yield and quality traits	1750:1778	milk yield and quality traits	1750:1778	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	2	88	dep	fat	536:538	arg1	nitrogen					583:590	milk urea nitrogen	573:590	milk urea nitrogen	573:590	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	88	dep	fat	536:538	arg1	contents					624:631	mineral contents	616:631	mineral contents	616:631	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	88	dep	fat	536:538	arg1	casein					556:561	casein	556:561	casein	556:561	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	88	dep	fat	536:538	arg1	lactose					564:570	lactose	564:570	lactose	564:570	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	88	dep	fat	536:538	arg1	fractions					601:609	protein fractions	593:609	protein fractions	593:609	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	88	dep	fat	536:538	arg1	protein					547:553	crude protein	541:553	crude protein	541:553	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	10	89	theme	high	1813:1816	arg1	>4.00					1823:1827	>4.00	1823:1827	>4.00	1823:1827	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	10	89	theme	high	1813:1816	arg1	SCS					1818:1820	high SCS	1813:1820	high SCS (>4.00)	1813:1828	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	2	90	from	composition	523:533	arg1	herds					732:736	multibreed herds	721:736	multibreed herds	721:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	91	theme	milk	573:576	arg1	nitrogen					583:590	milk urea nitrogen	573:590	milk urea nitrogen	573:590	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	6	92	theme	test	1184:1187	arg1	day					1189:1191	herd test day	1179:1191	herd test day	1179:1191	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	13	93	theme	lower	2327:2331	arg1	SCS					2333:2335	lower SCS	2327:2335	lower SCS	2327:2335	The best milk yield and quality were observed between SCS 0.00 and 1.00, but milk quality of Holstein Friesians started to decrease at lower SCS compared with milk quality of Brown Swiss and Simmental cows.					
31733847	0	94	dep	Swiss	117:121	arg1	breeds					164:169	cattle breeds	157:169	cattle breeds	157:169	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	7	95	theme	<	1423:1423	arg1	≤					1429:1429	-1.00 < SCS ≤ 0.00	1417:1434	-1.00 < SCS ≤ 0.00	1417:1434	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	7	95	theme	<	1423:1423	arg1	class					1410:1414	the reference class	1396:1414	the reference class (-1.00 < SCS ≤ 0.00)	1396:1435	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	14	96	theme	udder	2506:2510	arg1	problems					2519:2526	udder health problems	2506:2526	udder health problems	2506:2526	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	12	97	theme	yields	2107:2112	arg1	loss					2077:2080	the overall loss	2065:2080	the overall loss of fat and crude protein yields	2065:2112	Breeds responded similarly to the increase of SCS, but the overall loss of fat and crude protein yields, and several minerals among Holstein Friesian were lower with increasing SCS.					
31733847	0	98	theme	technological	87:99	arg1	traits					101:106	technological traits	87:106	technological traits	87:106	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	1	99	theme	low	297:299	arg1	SCC					301:303	low SCC	297:303	low SCC	297:303	High milk somatic cell count (SCC) influences milk production and quality; however, very little is known about the effect of low SCC on milk quality, especially in terms of mineral content and coagulation properties.					
31733847	13	100	theme	Friesians	2294:2302	arg1	quality					2274:2280	milk quality	2269:2280	milk quality of Holstein Friesians	2269:2302	The best milk yield and quality were observed between SCS 0.00 and 1.00, but milk quality of Holstein Friesians started to decrease at lower SCS compared with milk quality of Brown Swiss and Simmental cows.					
31733847	2	101	dep	composition	523:533	arg1	fat					536:538	fat	536:538	fat	536:538	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	8	102	theme	polynomial	1540:1549	arg1	regression					1551:1560	a third-order polynomial regression	1526:1560	a third-order polynomial regression	1526:1560	Results showed that the relationship between SCS and milk traits followed a third-order polynomial regression.					
31733847	2	103	theme	somatic	449:455	arg1	SCS					469:471	SCS	469:471	SCS	469:471	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	103	theme	somatic	449:455	arg1	score					462:466	somatic cell score	449:466	somatic cell score (SCS)	449:472	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	11	104	theme	present	1920:1926	arg1	SCS					1933:1935	present high SCS	1920:1935	present high SCS	1920:1935	Moreover, cows with SCS lower than -1.00 on a test day were about 7 times more likely to present high SCS within the following 90 ± 5 d than cows with SCS between -1.00 and 0.00.					
31733847	9	105	theme	yields	1612:1617	arg1	loss					1575:1578	The average loss	1563:1578	The average loss of milk, fat, and crude protein yields	1563:1617	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	9	105	theme	yields	1612:1617	arg1	kg/d					1645:1648	0.43, 0.01, and 0.01 kg/d	1624:1648	0.43, 0.01, and 0.01 kg/d	1624:1648	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	7	106	theme	reference	1400:1408	arg1	≤					1429:1429	-1.00 < SCS ≤ 0.00	1417:1434	-1.00 < SCS ≤ 0.00	1417:1434	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	7	106	theme	reference	1400:1408	arg1	class					1410:1414	the reference class	1396:1414	the reference class (-1.00 < SCS ≤ 0.00)	1396:1435	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	13	107	theme	milk	2269:2272	arg1	quality					2274:2280	milk quality	2269:2280	milk quality of Holstein Friesians	2269:2302	The best milk yield and quality were observed between SCS 0.00 and 1.00, but milk quality of Holstein Friesians started to decrease at lower SCS compared with milk quality of Brown Swiss and Simmental cows.					
31733847	9	108	theme	SCS	1674:1676	arg1	unit					1678:1681	each SCS unit	1669:1681	each SCS unit higher than 1.00	1669:1698	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	9	109	theme	higher	1683:1688	arg1	unit					1678:1681	each SCS unit	1669:1681	each SCS unit higher than 1.00	1669:1698	The average loss of milk, fat, and crude protein yields were 0.43, 0.01, and 0.01 kg/d, respectively, for each SCS unit higher than 1.00.					
31733847	0	110	theme	cell	19:22	arg1	score					24:28	somatic cell score	11:28	somatic cell score	11:28	Effects of somatic cell score on milk yield and mid-infrared predicted composition and technological traits of Brown Swiss, Holstein Friesian, and Simmental cattle breeds.					
31733847	11	111	theme	test	1877:1880	arg1	day					1882:1884	a test day	1875:1884	a test day	1875:1884	Moreover, cows with SCS lower than -1.00 on a test day were about 7 times more likely to present high SCS within the following 90 ± 5 d than cows with SCS between -1.00 and 0.00.					
31733847	7	112	theme	odds	1381:1384	arg1	ratios					1386:1391	odds ratios	1381:1391	odds ratios to the reference class (-1.00 < SCS ≤ 0.00)	1381:1435	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	6	113	theme	residual	1203:1210	arg1	effects					1222:1228	residual as random effects	1203:1228	residual as random effects	1203:1228	Statistical analysis was performed using a linear mixed model, which included breed, parity, stage of lactation, SCS class, and their interactions as fixed effects, and herd test day, cow, and residual as random effects.					
31733847	7	114	dep	4.00	1275:1278	arg1	d					1309:1309	5 d	1307:1309	5 d	1307:1309	The probability that cows experienced SCS > 4.00 at 30 ± 5, 60 ± 5, or 90 ± 5 d after the observation test day was calculated for each SCS class, and odds ratios to the reference class (-1.00 < SCS ≤ 0.00) were reported.					
31733847	3	115	theme	coagulation	760:770	arg1	traits					772:777	coagulation traits	760:777	coagulation traits	760:777	Milk composition and coagulation traits were predicted using mid-infrared spectroscopy.					
31733847	2	116	theme	present	399:405	arg1	study					407:411	the present study	395:411	the present study	395:411	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	4	117	from	observations	857:868	arg1	herds					902:906	313 multibreed herds	887:906	313 multibreed herds	887:906	The data set comprised 95,591 observations of 6,940 cows in 313 multibreed herds, collected from January 2011 to December 2017.					
31733847	10	118	from	effects	1739:1745	arg1	traits					1773:1778	milk yield and quality traits	1750:1778	milk yield and quality traits	1750:1778	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	12	119	theme	fat	2085:2087	arg1	loss					2077:2080	the overall loss	2065:2080	the overall loss of fat and crude protein yields	2065:2112	Breeds responded similarly to the increase of SCS, but the overall loss of fat and crude protein yields, and several minerals among Holstein Friesian were lower with increasing SCS.					
31733847	2	120	theme	coagulation	639:649	arg1	properties					651:660	coagulation properties	639:660	coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds	639:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	14	121	dep	indicator	2493:2501	arg1	used					2546:2549	used	2546:2549	may be used for early detection of mastitis	2539:2581	Results suggest a breed-dependent optimum of SCS, and highlighted that very low SCS can be an indicator of udder health problems and, thus, may be used for early detection of mastitis.					
31733847	11	122	theme	likely	1910:1915	arg1	times					1899:1903	about 7 times	1891:1903	about 7 times more likely to present high SCS	1891:1935	Moreover, cows with SCS lower than -1.00 on a test day were about 7 times more likely to present high SCS within the following 90 ± 5 d than cows with SCS between -1.00 and 0.00.					
31733847	12	123	theme	overall	2069:2075	arg1	loss					2077:2080	the overall loss	2065:2080	the overall loss of fat and crude protein yields	2065:2112	Breeds responded similarly to the increase of SCS, but the overall loss of fat and crude protein yields, and several minerals among Holstein Friesian were lower with increasing SCS.					
31733847	2	124	theme	Friesian	687:694	arg1	composition					523:533	composition	523:533	composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents)	523:632	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	124	theme	Friesian	687:694	arg1	yield					516:520	milk yield	511:520	milk yield	511:520	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	2	124	theme	Friesian	687:694	arg1	properties					651:660	coagulation properties	639:660	coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds	639:736	Thus, the present study aimed to investigate the effects of somatic cell score (SCS), calculated as log2(SCC/100) + 3, on milk yield, composition (fat, crude protein, casein, lactose, milk urea nitrogen, protein fractions, and mineral contents), and coagulation properties of Brown Swiss, Holstein Friesian, and Simmental cows from multibreed herds.					
31733847	10	125	contain	had	1723:1725	arg1	<-1.00					1715:1720	<-1.00	1715:1720	<-1.00	1715:1720	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	10	125	contain	had	1723:1725	arg1	SCS					1710:1712	Very low SCS	1701:1712	Very low SCS (<-1.00)	1701:1721	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
31733847	10	125	contain	had	1723:1725	arg2	effects					1739:1745	detrimental effects	1727:1745	detrimental effects on milk yield and quality traits	1727:1778	Very low SCS (<-1.00) had detrimental effects on milk yield and quality traits similar to or even stronger than high SCS (>4.00).					
30267755	11	0	dep	F0P70	2251:2255	arg1	groups					2269:2274	groups	2269:2274	groups	2269:2274	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	10	1	theme	superoxide	1920:1929	arg1	mtmnsod					1942:1948	mtmnsod	1942:1948	mtmnsod	1942:1948	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	10	1	theme	superoxide	1920:1929	arg1	dismutase					1931:1939	mitochondrial manganese superoxide dismutase	1896:1939	mitochondrial manganese superoxide dismutase (mtmnsod)	1896:1949	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	11	2	theme	cytosolic	2112:2120	arg1	cytmnsod					2154:2161	cytmnsod	2154:2161	cytmnsod	2154:2161	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	11	2	theme	cytosolic	2112:2120	arg1	dismutase					2143:2151	cytosolic manganese superoxide dismutase	2112:2151	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups	2108:2209	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	9	3	theme	50	1771:1772	arg1	%					1773:1773	%	1773:1773	%	1773:1773	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	9	4	theme	%	1773:1773	arg1	diet					1789:1792	50% plant protein diet	1771:1792	50% plant protein diet	1771:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	6	5	theme	other	1056:1060	arg1	groups					1062:1067	other groups	1056:1067	other groups	1056:1067	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	9	6	dep	affected	1689:1696	arg1	P < 0.05					1679:1686	P < 0.05	1679:1686	P < 0.05	1679:1686	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	9	6	dep	affected	1689:1696	arg1	whereas					1795:1801	whereas	1795:1801	whereas	1795:1801	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	1	7	theme	dietary	232:238	arg1	FOS					263:265	FOS	263:265	FOS	263:265	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	1	7	theme	dietary	232:238	arg1	fructooligosaccharide					240:260	dietary fructooligosaccharide	232:260	dietary fructooligosaccharide (FOS)	232:266	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	10	8	theme	mitochondrial	1896:1908	arg1	mtmnsod					1942:1948	mtmnsod	1942:1948	mtmnsod	1942:1948	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	10	8	theme	mitochondrial	1896:1908	arg1	dismutase					1931:1939	mitochondrial manganese superoxide dismutase	1896:1939	mitochondrial manganese superoxide dismutase (mtmnsod)	1896:1949	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	6	9	theme	F0.2P50	959:965	arg1	diet					967:970	F0.2P50 diet	959:970	F0.2P50 diet	959:970	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	9	10	theme	protein	1781:1787	arg1	diet					1789:1792	50% plant protein diet	1771:1792	50% plant protein diet	1771:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	8	11	theme	oxide	1424:1428	arg1	concentration					1435:1447	nitric oxide (NO) concentration	1417:1447	nitric oxide (NO) concentration	1417:1447	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	8	11	theme	oxide	1424:1428	arg1	Meanwhile					1406:1414	Meanwhile	1406:1414	Meanwhile	1406:1414	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	11	12	theme	superoxide	2132:2141	arg1	cytmnsod					2154:2161	cytmnsod	2154:2161	cytmnsod	2154:2161	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	11	12	theme	superoxide	2132:2141	arg1	dismutase					2143:2151	cytosolic manganese superoxide dismutase	2112:2151	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups	2108:2209	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	1	13	from	effect	222:227	arg1	immunity					338:345	immunity	338:345	immunity	338:345	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	1	13	from	effect	222:227	arg1	composition					302:312	whole body composition	291:312	whole body composition	291:312	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	1	13	from	effect	222:227	arg1	status					327:332	antioxidant status	315:332	antioxidant status	315:332	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	1	13	from	effect	222:227	arg1	performance					278:288	growth performance	271:288	growth performance	271:288	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	10	14	theme	protein	2092:2098	arg1	levels					2100:2105	both FOS and plant protein levels	2073:2105	levels	2100:2105	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	1	15	theme	plant	375:379	arg1	diets					389:393	plant protein diets	375:393	plant protein diets	375:393	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	8	16	theme	FOS	1533:1535	arg1	diets					1537:1541	0.2% FOS diets	1528:1541	0.2% FOS diets	1528:1541	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	4	17	theme	50	757:758	arg1	%					759:759	%	759:759	%	759:759	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	8	18	theme	acid	1450:1453	arg1	ACP					1468:1470	ACP	1468:1470	ACP	1468:1470	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	8	18	theme	acid	1450:1453	arg1	phosphatase					1455:1465	acid phosphatase	1450:1465	acid phosphatase (ACP)	1450:1471	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	7	19	from	activity	1205:1212	arg1	crabs					1217:1221	crabs	1217:1221	crabs fed F0.2P50	1217:1233	Catalase (CAT) activity in crabs fed F0.2P50 increased significantly (P < 0.05) compared with crabs fed F0P60, F0P70, F0.2P60 and F0.2P70, but malondialdehyde (MDA) concentrations decreased significantly (P < 0.05).					
30267755	4	20	theme	plant	761:765	arg1	level					775:779	50% plant protein level	757:779	50% plant protein level	757:779	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	2	21	theme	factorial	618:626	arg1	design					628:633	a 2 × 3 factorial design	610:633	a 2 × 3 factorial design	610:633	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	10	22	theme	manganese	1910:1918	arg1	mtmnsod					1942:1948	mtmnsod	1942:1948	mtmnsod	1942:1948	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	10	22	theme	manganese	1910:1918	arg1	dismutase					1931:1939	mitochondrial manganese superoxide dismutase	1896:1939	mitochondrial manganese superoxide dismutase (mtmnsod)	1896:1949	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	7	23	dep	increased	1235:1243	arg1	P < 0.05					1395:1402	P < 0.05	1395:1402	P < 0.05	1395:1402	Catalase (CAT) activity in crabs fed F0.2P50 increased significantly (P < 0.05) compared with crabs fed F0P60, F0P70, F0.2P60 and F0.2P70, but malondialdehyde (MDA) concentrations decreased significantly (P < 0.05).					
30267755	7	23	dep	increased	1235:1243	arg1	P < 0.05					1260:1267	P < 0.05	1260:1267	P < 0.05	1260:1267	Catalase (CAT) activity in crabs fed F0.2P50 increased significantly (P < 0.05) compared with crabs fed F0P60, F0P70, F0.2P60 and F0.2P70, but malondialdehyde (MDA) concentrations decreased significantly (P < 0.05).					
30267755	5	24	theme	highest	866:872	arg1	value					874:878	The highest value	862:878	The highest value in survival rate	862:895	The highest value in survival rate was observed in crabs fed F0.2P50 and F0.2P60 diet.					
30267755	7	25	theme	malondialdehyde	1333:1347	arg1	concentrations					1355:1368	malondialdehyde (MDA) concentrations	1333:1368	malondialdehyde (MDA) concentrations	1333:1368	Catalase (CAT) activity in crabs fed F0.2P50 increased significantly (P < 0.05) compared with crabs fed F0P60, F0P70, F0.2P60 and F0.2P70, but malondialdehyde (MDA) concentrations decreased significantly (P < 0.05).					
30267755	6	26	theme	protein	1006:1012	arg1	P < 0.05					1023:1030	P < 0.05	1023:1030	P < 0.05	1023:1030	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	6	26	theme	protein	1006:1012	arg1	content					1014:1020	significantly higher crude protein content	979:1020	significantly higher crude protein content (P < 0.05)	979:1031	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	9	27	theme	differentiation	1837:1851	arg1	myd88					1864:1868	Myeloid differentiation factor 88 (myd88)	1829:1869	Myeloid differentiation factor 88 (myd88)	1829:1869	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	8	28	theme	%	1603:1603	arg1	diets					1609:1613	0% FOS diets	1602:1613	0% FOS diets	1602:1613	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	0	29	theme	diet	133:136	arg1	level					110:114	a high level	103:114	a high level of plant protein diet	103:136	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	5	30	located	observed	901:908	arg2	value					874:878	The highest value	862:878	The highest value in survival rate	862:895	The highest value in survival rate was observed in crabs fed F0.2P50 and F0.2P60 diet.					
30267755	5	30	located	observed	901:908	arg1	crabs					913:917	crabs	913:917	crabs fed F0.2P50 and F0.2P60 diet	913:946	The highest value in survival rate was observed in crabs fed F0.2P50 and F0.2P60 diet.					
30267755	3	31	theme	protein	713:719	arg1	level					721:725	dietary plant protein level	699:725	dietary plant protein level	699:725	The results showed that weight gain increased significantly as dietary plant protein level decreased from 70% to 50%.					
30267755	8	32	theme	0.2	1528:1530	arg1	%					1531:1531	%	1531:1531	%	1531:1531	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	11	33	theme	F0.2P50	2184:2190	arg1	groups					2204:2209	F0.2P50 and F0.2P60 groups	2184:2209	F0.2P50 and F0.2P60 groups	2184:2209	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	6	34	theme	higher	993:998	arg1	P < 0.05					1023:1030	P < 0.05	1023:1030	P < 0.05	1023:1030	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	6	34	theme	higher	993:998	arg1	content					1014:1020	significantly higher crude protein content	979:1020	significantly higher crude protein content (P < 0.05)	979:1031	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	9	35	theme	prophenoloxidase	1635:1650	arg1	expressions					1620:1630	The expressions	1616:1630	The expressions of prophenoloxidase (propo)	1616:1658	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	1	36	theme	diets	389:393	arg1	levels					365:370	high levels	360:370	high levels of plant protein diets	360:393	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	8	37	theme	alkaline	1477:1484	arg1	AKP					1499:1501	AKP	1499:1501	AKP	1499:1501	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	8	37	theme	alkaline	1477:1484	arg1	phosphatase					1486:1496	alkaline phosphatase	1477:1496	alkaline phosphatase (AKP)	1477:1502	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	3	38	theme	dietary	699:705	arg1	level					721:725	dietary plant protein level	699:725	dietary plant protein level	699:725	The results showed that weight gain increased significantly as dietary plant protein level decreased from 70% to 50%.					
30267755	12	39	theme	higher	2465:2470	arg1	diets					2486:2490	higher plant protein diets	2465:2490	higher plant protein diets	2465:2490	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	6	40	theme	lipid	1147:1151	arg1	contents					1119:1126	the contents	1115:1126	the contents of moisture, crude lipid and ash	1115:1159	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	3	41	theme	70	742:743	arg1	%					744:744	%	744:744	%	744:744	The results showed that weight gain increased significantly as dietary plant protein level decreased from 70% to 50%.					
30267755	4	42	theme	%	801:801	arg1	FOS					803:805	0.2% FOS	798:805	0.2% FOS	798:805	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	0	43	theme	Chinese	79:85	arg1	crab					94:97	Chinese mitten crab	79:97	Chinese mitten crab fed a high level of plant protein diet	79:136	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	1	44	theme	antioxidant	315:325	arg1	status					327:332	antioxidant status	315:332	antioxidant status	315:332	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	1	45	theme	8-week	142:147	arg1	trial					157:161	An 8-week feeding trial	139:161	An 8-week feeding trial	139:161	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	0	46	theme	protein	125:131	arg1	diet					133:136	plant protein diet	119:136	plant protein diet	119:136	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	5	47	from	value	874:878	arg1	rate					892:895	survival rate	883:895	survival rate	883:895	The highest value in survival rate was observed in crabs fed F0.2P50 and F0.2P60 diet.					
30267755	2	48	dep	formulated	430:439	arg1	designated					442:451	designated	442:451	designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70	442:504	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	0	49	from	effects	56:62	arg1	crab					94:97	Chinese mitten crab	79:97	Chinese mitten crab fed a high level of plant protein diet	79:136	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	10	50	dep	affected	2050:2057	arg1	P < 0.05					2060:2067	P < 0.05	2060:2067	P < 0.05	2060:2067	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	2	51	theme	experimental	406:417	arg1	F0P50					456:460	F0P50	456:460	F0P50	456:460	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	2	51	theme	experimental	406:417	arg1	diets					419:423	six experimental diets	402:423	six experimental diets	402:423	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	1	52	theme	growth	271:276	arg1	performance					278:288	growth performance	271:288	growth performance	271:288	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	10	53	theme	mRNA	1876:1879	arg1	expressions					1881:1891	The mRNA expressions	1872:1891	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs)	1872:2029	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	12	54	theme	growth	2360:2365	arg1	performance					2367:2377	growth performance	2360:2377	growth performance in crabs fed lower plant protein diets	2360:2416	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	0	55	theme	Dietary	0:6	arg1	fructooligosaccharide					8:28	Dietary fructooligosaccharide	0:28	Dietary fructooligosaccharide	0:28	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	8	56	theme	%	1531:1531	arg1	diets					1537:1541	0.2% FOS diets	1528:1541	0.2% FOS diets	1528:1541	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	2	57	theme	plant	559:563	arg1	%					594:594	50, 60, or 70%	581:594	50, 60, or 70%	581:594	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	2	57	theme	plant	559:563	arg1	levels					573:578	three plant protein levels	553:578	three plant protein levels (50, 60, or 70%)	553:595	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	12	58	theme	lower	2392:2396	arg1	diets					2412:2416	lower plant protein diets	2392:2416	lower plant protein diets	2392:2416	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	9	59	theme	protein	1720:1726	arg1	levels					1728:1733	dietary plant protein levels	1706:1733	dietary plant protein levels with the highest values observed in 50% plant protein diet	1706:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	12	60	theme	protein	2404:2410	arg1	diets					2412:2416	lower plant protein diets	2392:2416	lower plant protein diets	2392:2416	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	0	61	theme	immunity	67:74	arg1	effects					56:62	the negative effects	43:62	the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet	43:136	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	6	62	theme	ash	1157:1159	arg1	contents					1119:1126	the contents	1115:1126	the contents of moisture, crude lipid and ash	1115:1159	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	1	63	theme	whole	291:295	arg1	composition					302:312	whole body composition	291:312	whole body composition	291:312	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	9	64	theme	highest	1744:1750	arg1	values					1752:1757	the highest values	1740:1757	the highest values observed in 50% plant protein diet	1740:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	12	65	from	performance	2367:2377	arg1	crabs					2382:2386	crabs	2382:2386	crabs fed lower plant protein diets	2382:2416	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	11	66	from	expressions	2169:2179	arg1	groups					2204:2209	F0.2P50 and F0.2P60 groups	2184:2209	F0.2P50 and F0.2P60 groups	2184:2209	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	11	67	theme	F0.2P60	2196:2202	arg1	groups					2204:2209	F0.2P50 and F0.2P60 groups	2184:2209	F0.2P50 and F0.2P60 groups	2184:2209	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	6	68	theme	crude	1141:1145	arg1	lipid					1147:1151	crude lipid	1141:1151	crude lipid	1141:1151	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	9	69	theme	dietary	1706:1712	arg1	levels					1728:1733	dietary plant protein levels	1706:1733	dietary plant protein levels with the highest values observed in 50% plant protein diet	1706:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	12	70	theme	0.2	2339:2341	arg1	%					2342:2342	%	2342:2342	%	2342:2342	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	2	71	theme	FOS	526:528	arg1	%					546:546	0 or 0.2%	538:546	0 or 0.2%	538:546	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	2	71	theme	FOS	526:528	arg1	levels					530:535	two FOS levels	522:535	two FOS levels (0 or 0.2%)	522:547	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	9	72	theme	plant	1775:1779	arg1	diet					1789:1792	50% plant protein diet	1771:1792	50% plant protein diet	1771:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	1	73	theme	fructooligosaccharide	240:260	arg1	effect					222:227	the effect	218:227	the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets	218:393	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	2	74	theme	2 × 3	612:616	arg1	design					628:633	a 2 × 3 factorial design	610:633	a 2 × 3 factorial design	610:633	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	8	75	theme	nitric	1417:1422	arg1	NO					1431:1432	NO	1431:1432	NO	1431:1432	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	8	75	theme	nitric	1417:1422	arg1	oxide					1424:1428	nitric oxide	1417:1428	nitric oxide (NO) concentration	1417:1447	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	1	76	theme	feeding	149:155	arg1	trial					157:161	An 8-week feeding trial	139:161	An 8-week feeding trial	139:161	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	12	77	theme	plant	2398:2402	arg1	diets					2412:2416	lower plant protein diets	2392:2416	lower plant protein diets	2392:2416	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	7	78	theme	Catalase	1190:1197	arg1	activity					1205:1212	Catalase (CAT) activity	1190:1212	Catalase (CAT) activity in crabs fed F0.2P50	1190:1233	Catalase (CAT) activity in crabs fed F0.2P50 increased significantly (P < 0.05) compared with crabs fed F0P60, F0P70, F0.2P60 and F0.2P70, but malondialdehyde (MDA) concentrations decreased significantly (P < 0.05).					
30267755	12	79	theme	%	2342:2342	arg1	FOS					2344:2346	0.2% FOS	2339:2346	0.2% FOS	2339:2346	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	10	80	theme	toll	2004:2007	arg1	expressions					1881:1891	The mRNA expressions	1872:1891	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs)	1872:2029	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	10	81	theme	dismutase	1931:1939	arg1	expressions					1881:1891	The mRNA expressions	1872:1891	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs)	1872:2029	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	9	82	with	levels	1728:1733	arg1	values					1752:1757	the highest values	1740:1757	the highest values observed in 50% plant protein diet	1740:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	10	83	theme	plant	2086:2090	arg1	levels					2100:2105	both FOS and plant protein levels	2073:2105	levels	2100:2105	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	10	84	theme	TNF-α	1979:1983	arg1	litaf					1993:1997	litaf	1993:1997	litaf	1993:1997	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	10	84	theme	TNF-α	1979:1983	arg1	factor					1985:1990	lipopolysaccharide-induced TNF-α factor	1952:1990	lipopolysaccharide-induced TNF-α factor (litaf)	1952:1998	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	8	85	theme	0	1602:1602	arg1	%					1603:1603	%	1603:1603	%	1603:1603	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	1	86	theme	high	360:363	arg1	levels					365:370	high levels	360:370	high levels of plant protein diets	360:393	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	1	87	theme	controlled	185:194	arg1	condition					196:204	controlled condition	185:204	controlled condition	185:204	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	4	88	theme	%	759:759	arg1	level					775:779	50% plant protein level	757:779	50% plant protein level	757:779	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	6	89	theme	significant	1088:1098	arg1	differences					1100:1110	no significant differences	1085:1110	no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05)	1085:1187	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	9	90	theme	Myeloid	1829:1835	arg1	myd88					1864:1868	Myeloid differentiation factor 88 (myd88)	1829:1869	Myeloid differentiation factor 88 (myd88)	1829:1869	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	8	91	theme	FOS	1605:1607	arg1	diets					1609:1613	0% FOS diets	1602:1613	0% FOS diets	1602:1613	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	8	92	theme	phosphatase	1455:1465	arg1	activities					1504:1513	acid phosphatase (ACP) and alkaline phosphatase (AKP) activities	1450:1513	acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets	1450:1541	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	8	92	theme	phosphatase	1455:1465	arg1	Meanwhile					1406:1414	Meanwhile	1406:1414	Meanwhile	1406:1414	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	12	93	from	results	2281:2287	arg1	study					2297:2301	this study	2292:2301	this study	2292:2301	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	10	94	theme	factor	1985:1990	arg1	expressions					1881:1891	The mRNA expressions	1872:1891	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs)	1872:2029	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	6	95	theme	crude	1000:1004	arg1	P < 0.05					1023:1030	P < 0.05	1023:1030	P < 0.05	1023:1030	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	6	95	theme	crude	1000:1004	arg1	content					1014:1020	significantly higher crude protein content	979:1020	significantly higher crude protein content (P < 0.05)	979:1031	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	2	96	contain	contain	514:520	arg2	%					546:546	0 or 0.2%	538:546	0 or 0.2%	538:546	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	2	96	contain	contain	514:520	arg2	%					594:594	50, 60, or 70%	581:594	50, 60, or 70%	581:594	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	2	96	contain	contain	514:520	arg2	levels					573:578	three plant protein levels	553:578	three plant protein levels (50, 60, or 70%)	553:595	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	2	96	contain	contain	514:520	arg2	levels					530:535	two FOS levels	522:535	two FOS levels (0 or 0.2%)	522:547	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	2	96	contain	contain	514:520	arg1	which					508:512	which	508:512	which	508:512	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	9	97	theme	factor	1853:1858	arg1	myd88					1864:1868	Myeloid differentiation factor 88 (myd88)	1829:1869	Myeloid differentiation factor 88 (myd88)	1829:1869	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	1	98	theme	protein	381:387	arg1	diets					389:393	plant protein diets	375:393	plant protein diets	375:393	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	11	99	theme	mRNA	2164:2167	arg1	expressions					2169:2179	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions	2108:2179	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups	2108:2209	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	11	99	theme	mRNA	2164:2167	arg1	higher					2230:2235	higher	2230:2235	higher	2230:2235	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	3	100	theme	weight	660:665	arg1	gain					667:670	weight gain	660:670	weight gain	660:670	The results showed that weight gain increased significantly as dietary plant protein level decreased from 70% to 50%.					
30267755	3	101	theme	plant	707:711	arg1	level					721:725	dietary plant protein level	699:725	dietary plant protein level	699:725	The results showed that weight gain increased significantly as dietary plant protein level decreased from 70% to 50%.					
30267755	12	102	with	supplementation	2318:2332	arg1	FOS					2344:2346	0.2% FOS	2339:2346	0.2% FOS	2339:2346	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	4	103	theme	protein	767:773	arg1	level					775:779	50% plant protein level	757:779	50% plant protein level	757:779	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	0	104	theme	mitten	87:92	arg1	crab					94:97	Chinese mitten crab	79:97	Chinese mitten crab fed a high level of plant protein diet	79:136	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	8	105	theme	phosphatase	1486:1496	arg1	activities					1504:1513	acid phosphatase (ACP) and alkaline phosphatase (AKP) activities	1450:1513	acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets	1450:1541	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	8	105	theme	phosphatase	1486:1496	arg1	Meanwhile					1406:1414	Meanwhile	1406:1414	Meanwhile	1406:1414	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	4	106	theme	0.2	798:800	arg1	%					801:801	%	801:801	%	801:801	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	9	107	located	observed	1759:1766	arg1	diet					1789:1792	50% plant protein diet	1771:1792	50% plant protein diet	1771:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	9	107	located	observed	1759:1766	arg2	values					1752:1757	the highest values	1740:1757	the highest values observed in 50% plant protein diet	1740:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	6	108	theme	moisture	1131:1138	arg1	contents					1119:1126	the contents	1115:1126	the contents of moisture, crude lipid and ash	1115:1159	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	8	109	dep	increased	1543:1551	arg1	P < 0.05					1568:1575	P < 0.05	1568:1575	P < 0.05	1568:1575	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	11	110	theme	manganese	2122:2130	arg1	cytmnsod					2154:2161	cytmnsod	2154:2161	cytmnsod	2154:2161	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	11	110	theme	manganese	2122:2130	arg1	dismutase					2143:2151	cytosolic manganese superoxide dismutase	2112:2151	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups	2108:2209	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	0	111	theme	high	105:108	arg1	level					110:114	a high level	103:114	a high level of plant protein diet	103:136	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	5	112	theme	survival	883:890	arg1	rate					892:895	survival rate	883:895	survival rate	883:895	The highest value in survival rate was observed in crabs fed F0.2P50 and F0.2P60 diet.					
30267755	1	113	theme	crabs	350:354	arg1	immunity					338:345	immunity	338:345	immunity	338:345	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	1	113	theme	crabs	350:354	arg1	composition					302:312	whole body composition	291:312	whole body composition	291:312	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	1	113	theme	crabs	350:354	arg1	status					327:332	antioxidant status	315:332	antioxidant status	315:332	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	1	113	theme	crabs	350:354	arg1	performance					278:288	growth performance	271:288	growth performance	271:288	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	8	114	theme	crabs	1518:1522	arg1	activities					1504:1513	acid phosphatase (ACP) and alkaline phosphatase (AKP) activities	1450:1513	acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets	1450:1541	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	8	114	theme	crabs	1518:1522	arg1	Meanwhile					1406:1414	Meanwhile	1406:1414	Meanwhile	1406:1414	Meanwhile, nitric oxide (NO) concentration, acid phosphatase (ACP) and alkaline phosphatase (AKP) activities of crabs fed 0.2% FOS diets increased significantly (P < 0.05) compared with crabs fed 0% FOS diets.					
30267755	3	115	theme	50	749:750	arg1	%					744:744	%	744:744	%	744:744	The results showed that weight gain increased significantly as dietary plant protein level decreased from 70% to 50%.					
30267755	4	116	theme	weight	833:838	arg1	P < 0.05					851:858	P < 0.05	851:858	P < 0.05	851:858	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	4	116	theme	weight	833:838	arg1	gain					840:843	weight gain	833:843	weight gain (WG) (P < 0.05)	833:859	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	4	116	theme	weight	833:838	arg1	WG					846:847	WG	846:847	WG	846:847	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	2	117	theme	protein	565:571	arg1	%					594:594	50, 60, or 70%	581:594	50, 60, or 70%	581:594	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	2	117	theme	protein	565:571	arg1	levels					573:578	three plant protein levels	553:578	three plant protein levels (50, 60, or 70%)	553:595	Thus, six experimental diets were formulated (designated as F0P50, F0P60, F0P70, F0.2P50, F0.2P60 and F0.2P70), which contain two FOS levels (0 or 0.2%) and three plant protein levels (50, 60, or 70%) according to a 2 × 3 factorial design.					
30267755	10	118	theme	lipopolysaccharide-induced	1952:1977	arg1	litaf					1993:1997	litaf	1993:1997	litaf	1993:1997	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	10	118	theme	lipopolysaccharide-induced	1952:1977	arg1	factor					1985:1990	lipopolysaccharide-induced TNF-α factor	1952:1990	lipopolysaccharide-induced TNF-α factor (litaf)	1952:1998	The mRNA expressions of mitochondrial manganese superoxide dismutase (mtmnsod), lipopolysaccharide-induced TNF-α factor (litaf) and toll like receptors (tlrs) were significantly affected (P < 0.05) by both FOS and plant protein levels.					
30267755	3	119	dep	%	744:744	arg1	to					746:747	to	746:747	to	746:747	The results showed that weight gain increased significantly as dietary plant protein level decreased from 70% to 50%.					
30267755	9	120	theme	plant	1714:1718	arg1	levels					1728:1733	dietary plant protein levels	1706:1733	dietary plant protein levels with the highest values observed in 50% plant protein diet	1706:1792	The expressions of prophenoloxidase (propo) was significantly (P < 0.05) affected only by dietary plant protein levels with the highest values observed in 50% plant protein diet, whereas the opposite was true for Myeloid differentiation factor 88 (myd88).					
30267755	0	121	theme	negative	47:54	arg1	effects					56:62	the negative effects	43:62	the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet	43:136	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	6	122	from	differences	1100:1110	arg1	contents					1119:1126	the contents	1115:1126	the contents of moisture, crude lipid and ash	1115:1159	Crabs fed F0.2P50 diet showed significantly higher crude protein content (P < 0.05) compared with those in other groups, but there were no significant differences in the contents of moisture, crude lipid and ash among all groups (P > 0.05).					
30267755	4	123	theme	FOS	803:805	arg1	addition					786:793	the addition	782:793	the addition of 0.2% FOS	782:805	At 50% plant protein level, the addition of 0.2% FOS can significantly elevate weight gain (WG) (P < 0.05).					
30267755	12	124	theme	plant	2472:2476	arg1	diets					2486:2490	higher plant protein diets	2465:2490	higher plant protein diets	2465:2490	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30267755	0	125	theme	plant	119:123	arg1	diet					133:136	plant protein diet	119:136	plant protein diet	119:136	Dietary fructooligosaccharide can mitigate the negative effects of immunity on Chinese mitten crab fed a high level of plant protein diet.					
30267755	11	126	theme	dismutase	2143:2151	arg1	expressions					2169:2179	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions	2108:2179	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups	2108:2209	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	11	126	theme	dismutase	2143:2151	arg1	higher					2230:2235	higher	2230:2235	higher	2230:2235	The cytosolic manganese superoxide dismutase (cytmnsod) mRNA expressions in F0.2P50 and F0.2P60 groups were significantly higher than those in F0P70 and F0.2P70 groups.					
30267755	1	127	theme	body	297:300	arg1	composition					302:312	whole body composition	291:312	whole body composition	291:312	An 8-week feeding trial was carried out under controlled condition to evaluate the effect of dietary fructooligosaccharide (FOS) on growth performance, whole body composition, antioxidant status and immunity of crabs fed high levels of plant protein diets.					
30267755	12	128	theme	protein	2478:2484	arg1	diets					2486:2490	higher plant protein diets	2465:2490	higher plant protein diets	2465:2490	The results in this study indicated that supplementation with 0.2% FOS can enhance growth performance in crabs fed lower plant protein diets and as well improve immunity in those fed with higher plant protein diets.					
30540710	6	0	theme	Enterobacteriaceae-related	928:953	arg1	bacteria					955:962	Enterobacteriaceae-related bacteria	928:962	Enterobacteriaceae-related bacteria	928:962	RESULTS The most abundant bacteria were Staphylococcus and Streptococcus, followed by Enterobacteriaceae-related bacteria.					
30540710	4	1	theme	Milk	565:568	arg1	microbiota					570:579	METHODS Milk microbiota	557:579	METHODS Milk microbiota from 25 healthy lactating women	557:611	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30540710	11	2	theme	lower	1520:1524	arg1	Actinobacteria					1526:1539	lower Actinobacteria	1520:1539	lower Actinobacteria	1520:1539	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	1	3	contain	contains	162:169	arg2	microbes					246:253	microbes	246:253	microbes	246:253	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	1	3	contain	contains	162:169	arg2	oligosaccharides					218:233	human milk oligosaccharides	207:233	human milk oligosaccharides (HMOs)	207:240	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	1	3	contain	contains	162:169	arg1	milk					157:160	BACKGROUND AND OBJECTIVES Breast milk	124:160	BACKGROUND AND OBJECTIVES Breast milk	124:160	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	1	3	contain	contains	162:169	arg2	factors					189:195	several bioactive factors	171:195	several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota	171:290	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	3	4	theme	lactation	517:525	arg1	association					535:545	the association	531:545	the association with HMO	531:554	This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.					
30540710	3	4	theme	lactation	517:525	arg1	month					508:512	the first month	498:512	the first month of lactation	498:525	This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.					
30540710	1	5	theme	infant	270:275	arg1	microbiota					281:290	the infant gut microbiota	266:290	the infant gut microbiota	266:290	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	4	6	theme	quantitative	631:642	arg1	reaction					661:668	quantitative polymerase chain reaction	631:668	quantitative polymerase chain reaction	631:668	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30540710	1	7	theme	bioactive	179:187	arg1	factors					189:195	several bioactive factors	171:195	several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota	171:290	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	1	7	theme	bioactive	179:187	arg1	microbes					246:253	microbes	246:253	microbes	246:253	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	1	7	theme	bioactive	179:187	arg1	oligosaccharides					218:233	human milk oligosaccharides	207:233	human milk oligosaccharides (HMOs)	207:240	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	4	8	theme	chain	655:659	arg1	reaction					661:668	quantitative polymerase chain reaction	631:668	quantitative polymerase chain reaction	631:668	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30540710	2	9	from	influence	354:362	arg1	microbiota					372:381	milk microbiota	367:381	milk microbiota	367:381	HMO profile is determined by secretor status; however, their influence on milk microbiota is still uncovered.					
30540710	4	10	from	women	607:611	arg1	microbiota					570:579	METHODS Milk microbiota	557:579	METHODS Milk microbiota from 25 healthy lactating women	557:611	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30540710	1	11	theme	milk	213:216	arg1	HMOs					236:239	HMOs	236:239	HMOs	236:239	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	1	11	theme	milk	213:216	arg1	oligosaccharides					218:233	human milk oligosaccharides	207:233	human milk oligosaccharides (HMOs)	207:240	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	12	12	theme	first	1758:1762	arg1	weeks					1766:1770	the first 4 weeks	1754:1770	the first 4 weeks	1754:1770	CONCLUSIONS Maternal secretor status is associated with the human milk microbiota composition and is maintained during the first 4 weeks.					
30540710	3	13	with	association	535:545	arg1	HMO					552:554	HMO	552:554	HMO	552:554	This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.					
30540710	13	14	theme	biological	1883:1892	arg1	impact					1894:1899	the potential biological impact	1869:1899	the potential biological impact on the neonate	1869:1914	Specific associations between milk microbiota, HMO, and secretor status were observed, although the potential biological impact on the neonate remains elusive.					
30540710	11	15	theme	Staphylococcaceae	1616:1632	arg1	Actinobacteria					1526:1539	lower Actinobacteria	1520:1539	lower Actinobacteria	1520:1539	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	11	15	theme	Staphylococcaceae	1616:1632	arg1	abundance					1561:1569	higher relative abundance	1545:1569	higher relative abundance	1545:1569	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	9	16	theme	secretor	1348:1355	arg1	samples					1357:1363	secretor samples	1348:1363	secretor samples	1348:1363	Lactobacillus spp, Enterococcus spp, and Streptococcus spp were lower in non-secretor than in secretor samples.					
30540710	8	17	from	Differences	1149:1159	arg1	composition					1175:1185	microbiota composition	1164:1185	microbiota composition	1164:1185	Differences in microbiota composition and quantity were found depending on secretor/non-secretor status.					
30540710	8	17	from	Differences	1149:1159	arg1	quantity					1191:1198	quantity	1191:1198	quantity	1191:1198	Differences in microbiota composition and quantity were found depending on secretor/non-secretor status.					
30540710	10	18	from	prevalent	1410:1418	arg1	samples					1436:1442	non-secretor samples	1423:1442	non-secretor samples	1423:1442	Bifidobacterium genus and species were less prevalent in non-secretor samples.					
30540710	5	19	theme	reaction-random	750:764	arg1	polymorphisms					782:794	polymerase chain reaction-random fragment length polymorphisms	733:794	polymerase chain reaction-random fragment length polymorphisms	733:794	Secretor genotype was obtained by polymerase chain reaction-random fragment length polymorphisms and by HMO identification and quantification.					
30540710	1	20	theme	Breast	150:155	arg1	milk					157:160	BACKGROUND AND OBJECTIVES Breast milk	124:160	BACKGROUND AND OBJECTIVES Breast milk	124:160	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	12	21	theme	milk	1701:1704	arg1	composition					1717:1727	the human milk microbiota composition	1691:1727	the human milk microbiota composition	1691:1727	CONCLUSIONS Maternal secretor status is associated with the human milk microbiota composition and is maintained during the first 4 weeks.					
30540710	4	22	theme	gene	678:681	arg1	pyrosequencing					683:696	16S gene pyrosequencing	674:696	16S gene pyrosequencing	674:696	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30540710	0	23	theme	Milk	77:80	arg1	Microbiota					82:91	Milk Microbiota	77:91	Milk Microbiota	77:91	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	11	24	from	differences	1456:1466	arg1	richness					1485:1492	richness	1485:1492	richness	1485:1492	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	11	24	from	differences	1456:1466	arg1	diversity					1471:1479	diversity	1471:1479	diversity	1471:1479	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	12	25	dep	CONCLUSIONS	1635:1645	arg1	associated					1675:1684	associated	1675:1684	is associated with the human milk microbiota composition	1672:1727	CONCLUSIONS Maternal secretor status is associated with the human milk microbiota composition and is maintained during the first 4 weeks.					
30540710	12	25	dep	CONCLUSIONS	1635:1645	arg1	maintained					1736:1745	maintained	1736:1745	is maintained during the first 4 weeks	1733:1770	CONCLUSIONS Maternal secretor status is associated with the human milk microbiota composition and is maintained during the first 4 weeks.					
30540710	7	26	theme	milk	997:1000	arg1	samples					1002:1008	secretor milk samples	988:1008	secretor milk samples	988:1008	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	11	27	theme	relative	1552:1559	arg1	abundance					1561:1569	higher relative abundance	1545:1569	higher relative abundance	1545:1569	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	0	28	theme	Observational	97:109	arg1	Study					117:121	An Observational Pilot Study	94:121	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.	0:122	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	13	29	from	impact	1894:1899	arg1	neonate					1908:1914	the neonate	1904:1914	the neonate	1904:1914	Specific associations between milk microbiota, HMO, and secretor status were observed, although the potential biological impact on the neonate remains elusive.					
30540710	1	30	theme	gut	277:279	arg1	microbiota					281:290	the infant gut microbiota	266:290	the infant gut microbiota	266:290	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	7	31	theme	predominant	969:979	arg1	HMO					981:983	The predominant HMO	965:983	The predominant HMO in secretor milk samples	965:1008	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	7	31	theme	predominant	969:979	arg1	I					1045:1045	2'FL and lacto-N-fucopentaose I	1015:1045	2'FL and lacto-N-fucopentaose I	1015:1045	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	10	32	from	samples	1436:1442	arg1	prevalent					1410:1418	prevalent	1410:1418	prevalent	1410:1418	Bifidobacterium genus and species were less prevalent in non-secretor samples.					
30540710	6	33	dep	RESULTS	842:848	arg1	bacteria					868:875	The most abundant bacteria	850:875	RESULTS The most abundant bacteria	842:875	RESULTS The most abundant bacteria were Staphylococcus and Streptococcus, followed by Enterobacteriaceae-related bacteria.					
30540710	0	34	theme	Maternal	15:22	arg1	Status					33:38	Maternal Secretor Status	15:38	Maternal Secretor Status	15:38	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	14	35	theme	nutrition	1979:1987	arg1	influence					1989:1997	the early nutrition influence	1969:1997	the early nutrition influence on the reduction of risk of disease	1969:2033	Future studies are needed to reveal the early nutrition influence on the reduction of risk of disease.					
30540710	0	36	theme	Status	33:38	arg1	Association					0:10	Association	0:10	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.	0:122	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	14	37	theme	Future	1933:1938	arg1	studies					1940:1946	Future studies	1933:1946	Future studies	1933:1946	Future studies are needed to reveal the early nutrition influence on the reduction of risk of disease.					
30540710	3	38	with	month	508:512	arg1	HMO					552:554	HMO	552:554	HMO	552:554	This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.					
30540710	12	39	theme	secretor	1656:1663	arg1	status					1665:1670	Maternal secretor status	1647:1670	Maternal secretor status	1647:1670	CONCLUSIONS Maternal secretor status is associated with the human milk microbiota composition and is maintained during the first 4 weeks.					
30540710	14	40	theme	disease	2027:2033	arg1	risk					2019:2022	risk	2019:2022	risk of disease	2019:2033	Future studies are needed to reveal the early nutrition influence on the reduction of risk of disease.					
30540710	0	41	theme	Oligosaccharides	55:70	arg1	Association					0:10	Association	0:10	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.	0:122	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	3	42	from	impact	440:445	arg1	microbiota					480:489	the milk microbiota	471:489	the milk microbiota	471:489	This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.					
30540710	0	43	theme	Human	44:48	arg1	Oligosaccharides					55:70	Human Milk Oligosaccharides	44:70	Human Milk Oligosaccharides	44:70	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	8	44	theme	microbiota	1164:1173	arg1	composition					1175:1185	microbiota composition	1164:1185	microbiota composition	1164:1185	Differences in microbiota composition and quantity were found depending on secretor/non-secretor status.					
30540710	6	45	theme	abundant	859:866	arg1	bacteria					868:875	The most abundant bacteria	850:875	RESULTS The most abundant bacteria	842:875	RESULTS The most abundant bacteria were Staphylococcus and Streptococcus, followed by Enterobacteriaceae-related bacteria.					
30540710	13	46	theme	Specific	1773:1780	arg1	associations					1782:1793	Specific associations	1773:1793	Specific associations between milk microbiota, HMO, and secretor status	1773:1843	Specific associations between milk microbiota, HMO, and secretor status were observed, although the potential biological impact on the neonate remains elusive.					
30540710	0	47	with	Association	0:10	arg1	Microbiota					82:91	Milk Microbiota	77:91	Milk Microbiota	77:91	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	3	48	theme	FUT2	454:457	arg1	genotype					459:466	the FUT2 genotype	450:466	the FUT2 genotype	450:466	This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.					
30540710	14	49	from	influence	1989:1997	arg1	reduction					2006:2014	the reduction	2002:2014	the reduction of risk of disease	2002:2033	Future studies are needed to reveal the early nutrition influence on the reduction of risk of disease.					
30540710	14	50	theme	early	1973:1977	arg1	influence					1989:1997	the early nutrition influence	1969:1997	the early nutrition influence on the reduction of risk of disease	1969:2033	Future studies are needed to reveal the early nutrition influence on the reduction of risk of disease.					
30540710	0	51	dep	Association	0:10	arg1	Study					117:121	An Observational Pilot Study	94:121	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.	0:122	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	3	52	theme	milk	475:478	arg1	microbiota					480:489	the milk microbiota	471:489	the milk microbiota	471:489	This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.					
30540710	2	53	theme	HMO	293:295	arg1	profile					297:303	HMO profile	293:303	HMO profile	293:303	HMO profile is determined by secretor status; however, their influence on milk microbiota is still uncovered.					
30540710	8	54	theme	secretor/non-secretor	1224:1244	arg1	status					1246:1251	secretor/non-secretor status	1224:1251	secretor/non-secretor status	1224:1251	Differences in microbiota composition and quantity were found depending on secretor/non-secretor status.					
30540710	4	55	theme	METHODS	557:563	arg1	microbiota					570:579	METHODS Milk microbiota	557:579	METHODS Milk microbiota from 25 healthy lactating women	557:611	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30540710	11	56	theme	non-secretor	1495:1506	arg1	samples					1508:1514	non-secretor samples	1495:1514	non-secretor samples	1495:1514	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	3	57	theme	first	502:506	arg1	month					508:512	the first month	498:512	the first month of lactation	498:525	This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.					
30540710	1	58	theme	several	171:177	arg1	factors					189:195	several bioactive factors	171:195	several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota	171:290	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	1	58	theme	several	171:177	arg1	microbes					246:253	microbes	246:253	microbes	246:253	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	1	58	theme	several	171:177	arg1	oligosaccharides					218:233	human milk oligosaccharides	207:233	human milk oligosaccharides (HMOs)	207:240	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	5	59	dep	HMO	803:805	arg1	quantification					826:839	quantification	826:839	quantification	826:839	Secretor genotype was obtained by polymerase chain reaction-random fragment length polymorphisms and by HMO identification and quantification.					
30540710	5	59	dep	HMO	803:805	arg1	identification					807:820	identification	807:820	identification	807:820	Secretor genotype was obtained by polymerase chain reaction-random fragment length polymorphisms and by HMO identification and quantification.					
30540710	11	60	contain	had	1516:1518	arg1	samples					1508:1514	non-secretor samples	1495:1514	non-secretor samples	1495:1514	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	11	60	contain	had	1516:1518	arg2	abundance					1561:1569	higher relative abundance	1545:1569	higher relative abundance	1545:1569	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	11	60	contain	had	1516:1518	arg2	Actinobacteria					1526:1539	lower Actinobacteria	1520:1539	lower Actinobacteria	1520:1539	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	5	61	theme	length	775:780	arg1	polymorphisms					782:794	polymerase chain reaction-random fragment length polymorphisms	733:794	polymerase chain reaction-random fragment length polymorphisms	733:794	Secretor genotype was obtained by polymerase chain reaction-random fragment length polymorphisms and by HMO identification and quantification.					
30540710	4	62	theme	polymerase	644:653	arg1	reaction					661:668	quantitative polymerase chain reaction	631:668	quantitative polymerase chain reaction	631:668	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30540710	1	63	theme	human	207:211	arg1	HMOs					236:239	HMOs	236:239	HMOs	236:239	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	1	63	theme	human	207:211	arg1	oligosaccharides					218:233	human milk oligosaccharides	207:233	human milk oligosaccharides (HMOs)	207:240	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	4	64	theme	lactating	597:605	arg1	women					607:611	25 healthy lactating women	586:611	25 healthy lactating women	586:611	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30540710	5	65	theme	Secretor	699:706	arg1	genotype					708:715	Secretor genotype	699:715	Secretor genotype	699:715	Secretor genotype was obtained by polymerase chain reaction-random fragment length polymorphisms and by HMO identification and quantification.					
30540710	14	66	theme	risk	2019:2022	arg1	reduction					2006:2014	the reduction	2002:2014	the reduction of risk of disease	2002:2033	Future studies are needed to reveal the early nutrition influence on the reduction of risk of disease.					
30540710	13	67	theme	potential	1873:1881	arg1	impact					1894:1899	the potential biological impact	1869:1899	the potential biological impact on the neonate	1869:1914	Specific associations between milk microbiota, HMO, and secretor status were observed, although the potential biological impact on the neonate remains elusive.					
30540710	7	68	theme	non-secretor	1056:1067	arg1	milk					1069:1072	non-secretor milk	1056:1072	non-secretor milk	1056:1072	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	7	69	from	HMO	981:983	arg1	samples					1002:1008	secretor milk samples	988:1008	secretor milk samples	988:1008	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	1	70	theme	BACKGROUND	124:133	arg1	milk					157:160	BACKGROUND AND OBJECTIVES Breast milk	124:160	BACKGROUND AND OBJECTIVES Breast milk	124:160	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	12	71	theme	human	1695:1699	arg1	composition					1717:1727	the human milk microbiota composition	1691:1727	the human milk microbiota composition	1691:1727	CONCLUSIONS Maternal secretor status is associated with the human milk microbiota composition and is maintained during the first 4 weeks.					
30540710	11	72	theme	Lactobacillaceae	1594:1609	arg1	Actinobacteria					1526:1539	lower Actinobacteria	1520:1539	lower Actinobacteria	1520:1539	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	11	72	theme	Lactobacillaceae	1594:1609	arg1	abundance					1561:1569	higher relative abundance	1545:1569	higher relative abundance	1545:1569	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	5	73	theme	chain	744:748	arg1	polymorphisms					782:794	polymerase chain reaction-random fragment length polymorphisms	733:794	polymerase chain reaction-random fragment length polymorphisms	733:794	Secretor genotype was obtained by polymerase chain reaction-random fragment length polymorphisms and by HMO identification and quantification.					
30540710	7	74	theme	lacto-N-fucopentaose	1024:1043	arg1	HMO					981:983	The predominant HMO	965:983	The predominant HMO in secretor milk samples	965:1008	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	7	74	theme	lacto-N-fucopentaose	1024:1043	arg1	I					1045:1045	2'FL and lacto-N-fucopentaose I	1015:1045	2'FL and lacto-N-fucopentaose I	1015:1045	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	1	75	theme	OBJECTIVES	139:148	arg1	milk					157:160	BACKGROUND AND OBJECTIVES Breast milk	124:160	BACKGROUND AND OBJECTIVES Breast milk	124:160	BACKGROUND AND OBJECTIVES Breast milk contains several bioactive factors including human milk oligosaccharides (HMOs) and microbes that shape the infant gut microbiota.					
30540710	12	76	theme	microbiota	1706:1715	arg1	composition					1717:1727	the human milk microbiota composition	1691:1727	the human milk microbiota composition	1691:1727	CONCLUSIONS Maternal secretor status is associated with the human milk microbiota composition and is maintained during the first 4 weeks.					
30540710	5	77	theme	fragment	766:773	arg1	polymorphisms					782:794	polymerase chain reaction-random fragment length polymorphisms	733:794	polymerase chain reaction-random fragment length polymorphisms	733:794	Secretor genotype was obtained by polymerase chain reaction-random fragment length polymorphisms and by HMO identification and quantification.					
30540710	7	78	theme	FL	1017:1018	arg1	HMO					981:983	The predominant HMO	965:983	The predominant HMO in secretor milk samples	965:1008	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	7	78	theme	FL	1017:1018	arg1	I					1045:1045	2'FL and lacto-N-fucopentaose I	1015:1045	2'FL and lacto-N-fucopentaose I	1015:1045	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	4	79	theme	16S	674:676	arg1	pyrosequencing					683:696	16S gene pyrosequencing	674:696	16S gene pyrosequencing	674:696	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30540710	7	80	dep	I	1045:1045	arg1	whereas					1048:1054	whereas	1048:1054	whereas	1048:1054	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	3	81	theme	genotype	459:466	arg1	impact					440:445	the impact	436:445	the impact of the FUT2 genotype on the milk microbiota	436:489	This study is aimed to determine the impact of the FUT2 genotype on the milk microbiota during the first month of lactation and the association with HMO.					
30540710	0	82	theme	Pilot	111:115	arg1	Study					117:121	An Observational Pilot Study	94:121	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.	0:122	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	11	83	theme	higher	1545:1550	arg1	abundance					1561:1569	higher relative abundance	1545:1569	higher relative abundance	1545:1569	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	10	84	theme	Bifidobacterium	1366:1380	arg1	genus					1382:1386	Bifidobacterium genus	1366:1386	Bifidobacterium genus	1366:1386	Bifidobacterium genus and species were less prevalent in non-secretor samples.					
30540710	7	85	theme	secretor	988:995	arg1	samples					1002:1008	secretor milk samples	988:1008	secretor milk samples	988:1008	The predominant HMO in secretor milk samples were 2'FL and lacto-N-fucopentaose I, whereas non-secretor milk was characterized by lacto-N-fucopentaose II and lacto-N-difucohexaose II.					
30540710	13	86	theme	secretor	1829:1836	arg1	status					1838:1843	secretor status	1829:1843	secretor status	1829:1843	Specific associations between milk microbiota, HMO, and secretor status were observed, although the potential biological impact on the neonate remains elusive.					
30540710	11	87	theme	Enterobacteriaceae	1574:1591	arg1	Actinobacteria					1526:1539	lower Actinobacteria	1520:1539	lower Actinobacteria	1520:1539	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	11	87	theme	Enterobacteriaceae	1574:1591	arg1	abundance					1561:1569	higher relative abundance	1545:1569	higher relative abundance	1545:1569	Despite no differences on diversity and richness, non-secretor samples had lower Actinobacteria and higher relative abundance of Enterobacteriaceae, Lactobacillaceae, and Staphylococcaceae.					
30540710	2	88	theme	milk	367:370	arg1	microbiota					372:381	milk microbiota	367:381	milk microbiota	367:381	HMO profile is determined by secretor status; however, their influence on milk microbiota is still uncovered.					
30540710	0	89	theme	Secretor	24:31	arg1	Status					33:38	Maternal Secretor Status	15:38	Maternal Secretor Status	15:38	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	13	90	theme	milk	1803:1806	arg1	microbiota					1808:1817	milk microbiota	1803:1817	milk microbiota	1803:1817	Specific associations between milk microbiota, HMO, and secretor status were observed, although the potential biological impact on the neonate remains elusive.					
30540710	5	91	theme	polymerase	733:742	arg1	polymorphisms					782:794	polymerase chain reaction-random fragment length polymorphisms	733:794	polymerase chain reaction-random fragment length polymorphisms	733:794	Secretor genotype was obtained by polymerase chain reaction-random fragment length polymorphisms and by HMO identification and quantification.					
30540710	0	92	theme	Milk	50:53	arg1	Oligosaccharides					55:70	Human Milk Oligosaccharides	44:70	Human Milk Oligosaccharides	44:70	Association of Maternal Secretor Status and Human Milk Oligosaccharides With Milk Microbiota: An Observational Pilot Study.					
30540710	12	93	theme	Maternal	1647:1654	arg1	status					1665:1670	Maternal secretor status	1647:1670	Maternal secretor status	1647:1670	CONCLUSIONS Maternal secretor status is associated with the human milk microbiota composition and is maintained during the first 4 weeks.					
30540710	10	94	theme	non-secretor	1423:1434	arg1	samples					1436:1442	non-secretor samples	1423:1442	non-secretor samples	1423:1442	Bifidobacterium genus and species were less prevalent in non-secretor samples.					
30540710	2	95	theme	secretor	322:329	arg1	status					331:336	secretor status	322:336	secretor status	322:336	HMO profile is determined by secretor status; however, their influence on milk microbiota is still uncovered.					
30540710	4	96	theme	healthy	589:595	arg1	women					607:611	25 healthy lactating women	586:611	25 healthy lactating women	586:611	METHODS Milk microbiota from 25 healthy lactating women was determined by quantitative polymerase chain reaction and 16S gene pyrosequencing.					
30728244	7	0	theme	free	1111:1114	arg1	GPIs					1116:1119	free GPIs	1111:1119	free GPIs	1111:1119	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	10	1	theme	free	1773:1776	arg1	GPIs					1778:1781	free GPIs	1773:1781	free GPIs in mammalian cells	1773:1800	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	9	2	theme	fourth	1520:1525	arg1	mannose					1527:1533	a fourth mannose	1518:1533	a fourth mannose	1518:1533	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	7	3	theme	fatty	1311:1315	arg1	remodeling					1322:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	2	4	theme	free	261:264	arg1	form					266:269	the free form	257:269	the free form on the mammalian cell surface	257:299	However, GPIs that exist in the free form on the mammalian cell surface remain largely unexplored.					
30728244	1	5	attach	linked	124:129	arg1	proteins					152:159	many cell-surface proteins	134:159	many cell-surface proteins	134:159	Glycosylphosphatidylinositols (GPIs) are linked to many cell-surface proteins, anchor these proteins in the membrane, and are well characterized.					
30728244	1	5	attach	linked	124:129	arg2	GPIs					114:117	GPIs	114:117	GPIs	114:117	Glycosylphosphatidylinositols (GPIs) are linked to many cell-surface proteins, anchor these proteins in the membrane, and are well characterized.					
30728244	1	5	attach	linked	124:129	arg2	Glycosylphosphatidylinositols					83:111	Glycosylphosphatidylinositols	83:111	Glycosylphosphatidylinositols (GPIs)	83:118	Glycosylphosphatidylinositols (GPIs) are linked to many cell-surface proteins, anchor these proteins in the membrane, and are well characterized.					
30728244	9	6	theme	glycan	1559:1564	arg1	anchor					1566:1571	phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ)	1538:1599	phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ)	1538:1599	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	6	7	theme	endoplasmic	1026:1036	arg1	reticulum					1038:1046	the endoplasmic reticulum	1022:1046	the endoplasmic reticulum	1022:1046	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	4	8	theme	side	615:618	arg1	chain					620:624	GalNAc side chain	608:624	the GalNAc side chain	604:624	We detected free GPIs bearing the GalNAc side chain on the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells.					
30728244	3	9	link	unlinked	451:458	arg1	GPIs					460:463	unlinked GPIs	451:463	unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus	451:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	2	10	theme	mammalian	278:286	arg1	surface					293:299	the mammalian cell surface	274:299	the mammalian cell surface	274:299	However, GPIs that exist in the free form on the mammalian cell surface remain largely unexplored.					
30728244	2	11	from	form	266:269	arg1	surface					293:299	the mammalian cell surface	274:299	the mammalian cell surface	274:299	However, GPIs that exist in the free form on the mammalian cell surface remain largely unexplored.					
30728244	3	12	from	GPIs	348:351	arg1	lines					370:374	cultured cell lines	356:374	cultured cell lines	356:374	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	3	12	from	GPIs	348:351	arg1	tissues					386:392	mouse tissues	380:392	mouse tissues	380:392	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	10	13	theme	free	1677:1680	arg1	GPIs					1682:1685	free GPIs	1677:1685	free GPIs	1677:1685	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	10	13	theme	free	1677:1680	arg1	components					1698:1707	normal components	1691:1707	normal components of the plasma membrane	1691:1730	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	5	14	theme	mouse	742:746	arg1	epididymis					794:803	epididymis	794:803	epididymis	794:803	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	14	theme	mouse	742:746	arg1	cord					780:783	spinal cord	773:783	spinal cord	773:783	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	14	theme	mouse	742:746	arg1	kidney					810:815	kidney	810:815	kidney	810:815	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	14	theme	mouse	742:746	arg1	pons					748:751	mouse pons	742:751	mouse pons	742:751	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	14	theme	mouse	742:746	arg1	testis					786:791	testis	786:791	testis	786:791	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	14	theme	mouse	742:746	arg1	oblongata					762:770	medulla oblongata	754:770	medulla oblongata	754:770	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	7	15	theme	phosphate	1246:1254	arg1	deacylation					1190:1200	protein 1 (PGAP1)-mediated inositol deacylation	1154:1200	protein 1 (PGAP1)-mediated inositol deacylation	1154:1200	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	7	15	theme	phosphate	1246:1254	arg1	remodeling					1322:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	7	15	theme	phosphate	1246:1254	arg1	removal					1218:1224	PGAP5-mediated removal	1203:1224	PGAP5-mediated removal of the ethanolamine phosphate from the second mannose	1203:1278	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	4	16	theme	Neuro2a	644:650	arg1	surface					633:639	the surface	629:639	the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells	629:700	We detected free GPIs bearing the GalNAc side chain on the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells.					
30728244	6	17	link	protein-linked	1077:1090	arg1	GPI					1092:1094	protein-linked GPI	1077:1094	protein-linked GPI	1077:1094	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	5	18	located	present	731:737	arg2	GPIs					721:724	free GPIs	716:724	free GPIs	716:724	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	18	located	present	731:737	arg1	epididymis					794:803	epididymis	794:803	epididymis	794:803	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	18	located	present	731:737	arg1	cord					780:783	spinal cord	773:783	spinal cord	773:783	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	18	located	present	731:737	arg1	kidney					810:815	kidney	810:815	kidney	810:815	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	18	located	present	731:737	arg1	pons					748:751	mouse pons	742:751	mouse pons	742:751	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	18	located	present	731:737	arg1	testis					786:791	testis	786:791	testis	786:791	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	18	located	present	731:737	arg1	oblongata					762:770	medulla oblongata	754:770	medulla oblongata	754:770	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	3	19	theme	first	531:535	arg1	mannose					537:543	the first mannose	527:543	the first mannose at the nonreducing terminus	527:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	1	20	theme	many	134:137	arg1	proteins					152:159	many cell-surface proteins	134:159	many cell-surface proteins	134:159	Glycosylphosphatidylinositols (GPIs) are linked to many cell-surface proteins, anchor these proteins in the membrane, and are well characterized.					
30728244	7	21	from	mannose	1272:1278	arg1	deacylation					1190:1200	protein 1 (PGAP1)-mediated inositol deacylation	1154:1200	protein 1 (PGAP1)-mediated inositol deacylation	1154:1200	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	7	21	from	mannose	1272:1278	arg1	remodeling					1322:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	7	21	from	mannose	1272:1278	arg1	removal					1218:1224	PGAP5-mediated removal	1203:1224	PGAP5-mediated removal of the ethanolamine phosphate from the second mannose	1203:1278	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	8	22	theme	T5	1344:1345	arg1	mAb					1347:1349	T5 mAb	1344:1349	T5 mAb	1344:1349	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	6	23	theme	protein-linked	1077:1090	arg1	GPI					1092:1094	protein-linked GPI	1077:1094	protein-linked GPI	1077:1094	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	5	24	from	present	731:737	arg1	epididymis					794:803	epididymis	794:803	epididymis	794:803	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	24	from	present	731:737	arg1	cord					780:783	spinal cord	773:783	spinal cord	773:783	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	24	from	present	731:737	arg1	kidney					810:815	kidney	810:815	kidney	810:815	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	24	from	present	731:737	arg1	pons					748:751	mouse pons	742:751	mouse pons	742:751	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	24	from	present	731:737	arg1	testis					786:791	testis	786:791	testis	786:791	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	24	from	present	731:737	arg1	oblongata					762:770	medulla oblongata	754:770	medulla oblongata	754:770	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	6	25	theme	plasma	1055:1060	arg1	membrane					1062:1069	the plasma membrane	1051:1069	the plasma membrane	1051:1069	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	9	26	theme	mAb-mediated	1614:1625	arg1	detection					1627:1635	T5 mAb-mediated detection	1611:1635	T5 mAb-mediated detection of free GPIs	1611:1648	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	3	27	theme	nonreducing	552:562	arg1	terminus					564:571	the nonreducing terminus	548:571	the nonreducing terminus	548:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	5	28	from	pons	748:751	arg1	present					731:737	present	731:737	present	731:737	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	29	theme	spinal	773:778	arg1	cord					780:783	spinal cord	773:783	spinal cord	773:783	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	29	theme	spinal	773:778	arg1	pons					748:751	mouse pons	742:751	mouse pons	742:751	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	6	30	theme	cells	857:861	arg1	panel					826:830	a panel	824:830	a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway	824:923	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	9	31	theme	GPIs	1645:1648	arg1	detection					1627:1635	T5 mAb-mediated detection	1611:1635	T5 mAb-mediated detection of free GPIs	1611:1648	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	3	32	contain	having	465:470	arg1	GPIs					460:463	unlinked GPIs	451:463	unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus	451:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	3	32	contain	having	465:470	arg2	chain					511:515	an N-acetylgalactosamine (GalNAc) side chain	472:515	an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus	472:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	3	33	used	used	403:406	arg2	we					400:401	we	400:401	we	400:401	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	6	34	theme	hamster	843:849	arg1	cells					857:861	Chinese hamster ovary cells	835:861	Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway	835:923	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	8	35	theme	inositol-linked	1384:1398	arg1	chain					1405:1409	the inositol-linked acyl chain	1380:1409	the inositol-linked acyl chain	1380:1409	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	6	36	from	reticulum	1038:1046	arg1	passage					1009:1015	passage	1009:1015	passage from the endoplasmic reticulum to the plasma membrane	1009:1069	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	7	37	theme	PGAP5-mediated	1203:1216	arg1	removal					1218:1224	PGAP5-mediated removal	1203:1224	PGAP5-mediated removal of the ethanolamine phosphate from the second mannose	1203:1278	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	6	38	from	defective	863:871	arg1	pathway					917:923	GPI remodeling pathway	902:923	GPI remodeling pathway	902:923	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	6	38	from	defective	863:871	arg1	GPI-transamidase					881:896	GPI-transamidase	881:896	GPI-transamidase	881:896	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	0	39	theme	Free	0:3	arg1	glycosylphosphatidylinositols					15:43	Free, unlinked glycosylphosphatidylinositols	0:43	Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces	0:70	Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces revisited.					
30728244	3	40	theme	cultured	356:363	arg1	lines					370:374	cultured cell lines	356:374	cultured cell lines	356:374	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	8	41	theme	ethanolamine-phosphate	1414:1435	arg1	chain					1442:1446	ethanolamine-phosphate side chain	1414:1446	ethanolamine-phosphate side chain	1414:1446	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	3	42	theme	T5	425:426	arg1	mAb					420:422	the T5-4E10 mAb	408:422	the T5-4E10 mAb (T5 mAb)	408:431	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	3	42	theme	T5	425:426	arg1	mAb					428:430	T5 mAb	425:430	T5 mAb	425:430	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	7	43	theme	-mediated	1171:1179	arg1	deacylation					1190:1200	protein 1 (PGAP1)-mediated inositol deacylation	1154:1200	protein 1 (PGAP1)-mediated inositol deacylation	1154:1200	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	10	44	theme	membrane	1723:1730	arg1	GPIs					1682:1685	free GPIs	1677:1685	free GPIs	1677:1685	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	10	44	theme	membrane	1723:1730	arg1	components					1698:1707	normal components	1691:1707	normal components of the plasma membrane	1691:1730	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	9	45	dep	anchor	1566:1571	arg1	Z					1592:1592	biosynthesis class Z	1573:1592	phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ)	1538:1599	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	9	45	dep	anchor	1566:1571	arg1	PIGZ					1595:1598	PIGZ	1595:1598	PIGZ	1595:1598	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	6	46	theme	GPI	902:904	arg1	pathway					917:923	GPI remodeling pathway	902:923	GPI remodeling pathway	902:923	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	3	47	theme	mouse	380:384	arg1	tissues					386:392	mouse tissues	380:392	mouse tissues	380:392	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	0	48	theme	cell	58:61	arg1	surfaces					63:70	mammalian cell surfaces	48:70	mammalian cell surfaces	48:70	Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces revisited.					
30728244	5	49	attach	present	731:737	arg2	GPIs					721:724	free GPIs	716:724	free GPIs	716:724	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	49	attach	present	731:737	arg1	epididymis					794:803	epididymis	794:803	epididymis	794:803	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	49	attach	present	731:737	arg1	cord					780:783	spinal cord	773:783	spinal cord	773:783	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	49	attach	present	731:737	arg1	kidney					810:815	kidney	810:815	kidney	810:815	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	49	attach	present	731:737	arg1	pons					748:751	mouse pons	742:751	mouse pons	742:751	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	49	attach	present	731:737	arg1	testis					786:791	testis	786:791	testis	786:791	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	49	attach	present	731:737	arg1	oblongata					762:770	medulla oblongata	754:770	medulla oblongata	754:770	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	8	50	theme	second	1462:1467	arg1	mannose					1469:1475	the second mannose	1458:1475	the second mannose	1458:1475	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	4	51	theme	C2C12	690:694	arg1	cells					696:700	HEK293, K562, and C2C12 cells	672:700	cells	696:700	We detected free GPIs bearing the GalNAc side chain on the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells.					
30728244	6	52	theme	structural	972:981	arg1	pathway					994:1000	the same structural remodeling pathway	963:1000	the same structural remodeling pathway	963:1000	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	0	53	dep	Free	0:3	arg1	unlinked					6:13	unlinked	6:13	unlinked	6:13	Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces revisited.					
30728244	7	54	theme	post-GPI	1131:1138	arg1	attachment					1140:1149	post-GPI attachment	1131:1149	post-GPI attachment	1131:1149	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	3	55	theme	unlinked	451:458	arg1	GPIs					460:463	unlinked GPIs	451:463	unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus	451:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	8	56	link	inositol-linked	1384:1398	arg1	chain					1405:1409	the inositol-linked acyl chain	1380:1409	the inositol-linked acyl chain	1380:1409	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	9	57	theme	mannose	1527:1533	arg1	addition					1506:1513	addition	1506:1513	addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ)	1506:1599	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	7	58	theme	acid	1317:1320	arg1	remodeling					1322:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	9	59	theme	phosphatidylinositol	1538:1557	arg1	anchor					1566:1571	phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ)	1538:1599	phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ)	1538:1599	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	7	60	theme	PGAP2-mediated	1296:1309	arg1	remodeling					1322:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	10	61	theme	mammalian	1786:1794	arg1	cells					1796:1800	mammalian cells	1786:1800	mammalian cells	1786:1800	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	5	62	theme	medulla	754:760	arg1	pons					748:751	mouse pons	742:751	mouse pons	742:751	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	5	62	theme	medulla	754:760	arg1	oblongata					762:770	medulla oblongata	754:770	medulla oblongata	754:770	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	6	63	theme	Chinese	835:841	arg1	cells					857:861	Chinese hamster ovary cells	835:861	Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway	835:923	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	7	64	theme	PGAP3-	1285:1290	arg1	remodeling					1322:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	PGAP3- and PGAP2-mediated fatty acid remodeling	1285:1331	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	4	65	theme	GalNAc	608:613	arg1	chain					620:624	GalNAc side chain	608:624	the GalNAc side chain	604:624	We detected free GPIs bearing the GalNAc side chain on the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells.					
30728244	6	66	from	pathway	917:923	arg1	defective					863:871	defective	863:871	defective	863:871	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	2	67	theme	cell	288:291	arg1	surface					293:299	the mammalian cell surface	274:299	the mammalian cell surface	274:299	However, GPIs that exist in the free form on the mammalian cell surface remain largely unexplored.					
30728244	10	68	from	GPIs	1778:1781	arg1	cells					1796:1800	mammalian cells	1786:1800	mammalian cells	1786:1800	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	9	69	theme	class	1586:1590	arg1	Z					1592:1592	biosynthesis class Z	1573:1592	phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ)	1538:1599	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	9	69	theme	class	1586:1590	arg1	PIGZ					1595:1598	PIGZ	1595:1598	PIGZ	1595:1598	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	7	70	theme	second	1265:1270	arg1	mannose					1272:1278	the second mannose	1261:1278	the second mannose	1261:1278	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	4	71	theme	free	586:589	arg1	GPIs					591:594	free GPIs	586:594	free GPIs bearing the GalNAc side chain	586:624	We detected free GPIs bearing the GalNAc side chain on the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells.					
30728244	9	72	theme	biosynthesis	1573:1584	arg1	Z					1592:1592	biosynthesis class Z	1573:1592	phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ)	1538:1599	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	9	72	theme	biosynthesis	1573:1584	arg1	PIGZ					1595:1598	PIGZ	1595:1598	PIGZ	1595:1598	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	8	73	attach	linked	1448:1453	arg2	chain					1405:1409	the inositol-linked acyl chain	1380:1409	the inositol-linked acyl chain	1380:1409	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	8	73	attach	linked	1448:1453	arg1	mannose					1469:1475	the second mannose	1458:1475	the second mannose	1458:1475	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	1	74	theme	cell-surface	139:150	arg1	proteins					152:159	many cell-surface proteins	134:159	many cell-surface proteins	134:159	Glycosylphosphatidylinositols (GPIs) are linked to many cell-surface proteins, anchor these proteins in the membrane, and are well characterized.					
30728244	3	75	theme	side	506:509	arg1	chain					511:515	an N-acetylgalactosamine (GalNAc) side chain	472:515	an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus	472:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	9	76	theme	T5	1611:1612	arg1	detection					1627:1635	T5 mAb-mediated detection	1611:1635	T5 mAb-mediated detection of free GPIs	1611:1648	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	8	77	theme	free	1362:1365	arg1	GPIs					1367:1370	free GPIs	1362:1370	free GPIs	1362:1370	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	7	78	theme	ethanolamine	1233:1244	arg1	phosphate					1246:1254	the ethanolamine phosphate	1229:1254	the ethanolamine phosphate	1229:1254	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	6	79	dep	do	1074:1075	arg1	as					1071:1072	as	1071:1072	as	1071:1072	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	10	80	theme	normal	1691:1696	arg1	GPIs					1682:1685	free GPIs	1677:1685	free GPIs	1677:1685	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	10	80	theme	normal	1691:1696	arg1	components					1698:1707	normal components	1691:1707	normal components of the plasma membrane	1691:1730	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	0	81	from	glycosylphosphatidylinositols	15:43	arg1	surfaces					63:70	mammalian cell surfaces	48:70	mammalian cell surfaces	48:70	Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces revisited.					
30728244	6	82	theme	defective	863:871	arg1	cells					857:861	Chinese hamster ovary cells	835:861	Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway	835:923	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	9	83	theme	free	1640:1643	arg1	GPIs					1645:1648	free GPIs	1640:1648	free GPIs	1640:1648	In contrast, addition of a fourth mannose by phosphatidylinositol glycan anchor biosynthesis class Z (PIGZ) inhibited T5 mAb-mediated detection of free GPIs.					
30728244	6	84	theme	ovary	851:855	arg1	cells					857:861	Chinese hamster ovary cells	835:861	Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway	835:923	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	8	85	theme	acyl	1400:1403	arg1	chain					1405:1409	the inositol-linked acyl chain	1380:1409	the inositol-linked acyl chain	1380:1409	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	6	86	from	GPI-transamidase	881:896	arg1	defective					863:871	defective	863:871	defective	863:871	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	4	87	theme	HEK293	672:677	arg1	K562					680:683	HEK293, K562, and C2C12 cells	672:700	K562	680:683	We detected free GPIs bearing the GalNAc side chain on the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells.					
30728244	1	88	from	anchor	162:167	arg1	membrane					191:198	the membrane	187:198	the membrane	187:198	Glycosylphosphatidylinositols (GPIs) are linked to many cell-surface proteins, anchor these proteins in the membrane, and are well characterized.					
30728244	3	89	attach	linked	517:522	arg1	mannose					537:543	the first mannose	527:543	the first mannose at the nonreducing terminus	527:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	3	89	attach	linked	517:522	arg2	chain					511:515	an N-acetylgalactosamine (GalNAc) side chain	472:515	an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus	472:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	6	90	theme	free	946:949	arg1	GPIs					951:954	free GPIs	946:954	free GPIs	946:954	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	3	91	theme	free	343:346	arg1	GPIs					348:351	free GPIs	343:351	free GPIs in cultured cell lines and mouse tissues	343:392	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	4	92	theme	K562	680:683	arg1	surface					633:639	the surface	629:639	the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells	629:700	We detected free GPIs bearing the GalNAc side chain on the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells.					
30728244	3	93	from	terminus	564:571	arg1	mannose					537:543	the first mannose	527:543	the first mannose at the nonreducing terminus	527:571	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	3	94	theme	T5-4E10	412:418	arg1	mAb					420:422	the T5-4E10 mAb	408:422	the T5-4E10 mAb (T5 mAb)	408:431	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	3	94	theme	T5-4E10	412:418	arg1	mAb					428:430	T5 mAb	425:430	T5 mAb	425:430	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	7	95	theme	inositol	1181:1188	arg1	deacylation					1190:1200	protein 1 (PGAP1)-mediated inositol deacylation	1154:1200	protein 1 (PGAP1)-mediated inositol deacylation	1154:1200	Specifically, free GPIs underwent post-GPI attachment to protein 1 (PGAP1)-mediated inositol deacylation, PGAP5-mediated removal of the ethanolamine phosphate from the second mannose, and PGAP3- and PGAP2-mediated fatty acid remodeling.					
30728244	8	96	theme	side	1437:1440	arg1	chain					1442:1446	ethanolamine-phosphate side chain	1414:1446	ethanolamine-phosphate side chain	1414:1446	Moreover, T5 mAb recognized free GPIs even if the inositol-linked acyl chain or ethanolamine-phosphate side chain linked to the second mannose is not removed.					
30728244	5	97	theme	free	716:719	arg1	GPIs					721:724	free GPIs	716:724	free GPIs	716:724	Furthermore, free GPIs were present in mouse pons, medulla oblongata, spinal cord, testis, epididymis, and kidney.					
30728244	3	98	theme	cell	365:368	arg1	lines					370:374	cultured cell lines	356:374	cultured cell lines	356:374	To investigate free GPIs in cultured cell lines and mouse tissues, here we used the T5-4E10 mAb (T5 mAb), which recognizes unlinked GPIs having an N-acetylgalactosamine (GalNAc) side chain linked to the first mannose at the nonreducing terminus.					
30728244	0	99	theme	mammalian	48:56	arg1	surfaces					63:70	mammalian cell surfaces	48:70	mammalian cell surfaces	48:70	Free, unlinked glycosylphosphatidylinositols on mammalian cell surfaces revisited.					
30728244	10	100	from	components	1698:1707	arg1	tissues					1740:1746	some tissues	1735:1746	some tissues	1735:1746	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	6	101	theme	remodeling	906:915	arg1	pathway					917:923	GPI remodeling pathway	902:923	GPI remodeling pathway	902:923	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	10	102	theme	plasma	1716:1721	arg1	membrane					1723:1730	the plasma membrane	1712:1730	the plasma membrane	1712:1730	Our results indicate that free GPIs are normal components of the plasma membrane in some tissues and further characterize free GPIs in mammalian cells.					
30728244	6	103	theme	remodeling	983:992	arg1	pathway					994:1000	the same structural remodeling pathway	963:1000	the same structural remodeling pathway	963:1000	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	6	104	theme	same	967:970	arg1	pathway					994:1000	the same structural remodeling pathway	963:1000	the same structural remodeling pathway	963:1000	Using a panel of Chinese hamster ovary cells defective in both GPI-transamidase and GPI remodeling pathway, we demonstrate that free GPIs follow the same structural remodeling pathway during passage from the endoplasmic reticulum to the plasma membrane as do protein-linked GPI.					
30728244	4	105	theme	cells	696:700	arg1	surface					633:639	the surface	629:639	the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells	629:700	We detected free GPIs bearing the GalNAc side chain on the surface of Neuro2a and CHO, but not of HEK293, K562, and C2C12 cells.					
30458190	6	0	theme	supramolecular	587:600	arg1	complexes					612:620	supramolecular inclusion complexes	587:620	supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2	587:664	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	1	1	theme	ethanol	215:221	arg1	extraction					223:232	ethanol extraction	215:232	ethanol extraction	215:232	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.)					
30458190	6	2	from	changes	454:460	arg1	shifts					474:479	chemical shifts	465:479	chemical shifts of protons of substrates and receptors	465:518	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	6	3	theme	protons	484:490	arg1	shifts					474:479	chemical shifts	465:479	chemical shifts of protons of substrates and receptors	465:518	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	5	4	with	complexation	348:359	arg1	β-cyclodextrin					390:403	β-cyclodextrin	390:403	β-cyclodextrin	390:403	The complexation of the phytoecdysteroid with β-cyclodextrin was studied by NMR spectroscopy.					
30458190	7	5	theme	higher	722:727	arg1	solubility					729:738	100 times higher solubility	712:738	100 times higher solubility in water	712:747	Ecdysterone-β-cyclodextrin complexes exhibit 100 times higher solubility in water than the parent compound.					
30458190	5	6	theme	NMR	420:422	arg1	spectroscopy					424:435	NMR spectroscopy	420:435	NMR spectroscopy	420:435	The complexation of the phytoecdysteroid with β-cyclodextrin was studied by NMR spectroscopy.					
30458190	6	7	theme	chemical	465:472	arg1	shifts					474:479	chemical shifts	465:479	chemical shifts of protons of substrates and receptors	465:518	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	1	8	theme	20-Hydroxyecdysterone	89:109	arg1	-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one					127:172	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one	89:172	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one	89:172	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.)					
30458190	1	9	theme	aerial	243:248	arg1	part					250:253	the aerial part	239:253	the aerial part of Silene wolgensis	239:273	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.)					
30458190	7	10	theme	Ecdysterone-β-cyclodextrin	667:692	arg1	complexes					694:702	Ecdysterone-β-cyclodextrin complexes	667:702	Ecdysterone-β-cyclodextrin complexes	667:702	Ecdysterone-β-cyclodextrin complexes exhibit 100 times higher solubility in water than the parent compound.					
30458190	1	11	dep	isolated	178:185	arg1	Hornem					276:281	Hornem	276:281	Hornem	276:281	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.)					
30458190	6	12	theme	1:1	655:657	arg1	composition					640:650	stoichiometric composition	625:650	stoichiometric composition of 1:1 or 1:2	625:664	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	4	13	theme	ex	333:334	arg1	Spreng					336:341	ex Spreng	333:341	ex Spreng.	333:342	ex Spreng.					
30458190	2	14	dep	wolgensis	308:316	arg1	Willd					319:323	Willd	319:323	Willd	319:323	Otth; sometimes Silene wolgensis (Willd.)					
30458190	0	15	theme	Bioavailability	0:14	arg1	study					31:35	Bioavailability and structural study	0:35	Bioavailability and structural study of 20-hydroxyecdysone	0:57	Bioavailability and structural study of 20-hydroxyecdysone complexes with cyclodextrins.					
30458190	5	16	theme	phytoecdysteroid	368:383	arg1	complexation					348:359	The complexation	344:359	The complexation of the phytoecdysteroid with β-cyclodextrin	344:403	The complexation of the phytoecdysteroid with β-cyclodextrin was studied by NMR spectroscopy.					
30458190	0	17	with	complexes	59:67	arg1	cyclodextrins					74:86	cyclodextrins	74:86	cyclodextrins	74:86	Bioavailability and structural study of 20-hydroxyecdysone complexes with cyclodextrins.					
30458190	1	18	theme	Silene	258:263	arg1	wolgensis					265:273	Silene wolgensis	258:273	Silene wolgensis	258:273	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.)					
30458190	0	19	theme	20-hydroxyecdysone	40:57	arg1	study					31:35	Bioavailability and structural study	0:35	Bioavailability and structural study of 20-hydroxyecdysone	0:57	Bioavailability and structural study of 20-hydroxyecdysone complexes with cyclodextrins.					
30458190	1	20	theme	wolgensis	265:273	arg1	part					250:253	the aerial part	239:253	the aerial part of Silene wolgensis	239:273	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.)					
30458190	2	21	dep	Otth	285:288	arg1	wolgensis					308:316	Silene wolgensis	301:316	Otth; sometimes Silene wolgensis (Willd.)	285:325	Otth; sometimes Silene wolgensis (Willd.)					
30458190	6	22	theme	composition	640:650	arg1	complexes					612:620	supramolecular inclusion complexes	587:620	supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2	587:664	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	6	23	theme	stoichiometric	625:638	arg1	composition					640:650	stoichiometric composition	625:650	stoichiometric composition of 1:1 or 1:2	625:664	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	6	24	theme	receptors	510:518	arg1	protons					484:490	protons	484:490	protons of substrates and receptors	484:518	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	7	25	from	solubility	729:738	arg1	water					743:747	water	743:747	water	743:747	Ecdysterone-β-cyclodextrin complexes exhibit 100 times higher solubility in water than the parent compound.					
30458190	7	26	theme	parent	758:763	arg1	compound					765:772	the parent compound	754:772	the parent compound	754:772	Ecdysterone-β-cyclodextrin complexes exhibit 100 times higher solubility in water than the parent compound.					
30458190	6	27	theme	substrates	495:504	arg1	protons					484:490	protons	484:490	protons of substrates and receptors	484:518	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	2	28	theme	Silene	301:306	arg1	wolgensis					308:316	Silene wolgensis	301:316	Otth; sometimes Silene wolgensis (Willd.)	285:325	Otth; sometimes Silene wolgensis (Willd.)					
30458190	1	29	theme	satisfactory	190:201	arg1	yield					203:207	satisfactory yield	190:207	satisfactory yield using ethanol extraction	190:232	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.)					
30458190	6	30	theme	1:2	662:664	arg1	composition					640:650	stoichiometric composition	625:650	stoichiometric composition of 1:1 or 1:2	625:664	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	6	31	theme	inclusion	602:610	arg1	complexes					612:620	supramolecular inclusion complexes	587:620	supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2	587:664	By studying the changes in chemical shifts of protons of substrates and receptors it was found that ecdysterone interacts with cyclodextrins to form supramolecular inclusion complexes of stoichiometric composition of 1:1 or 1:2.					
30458190	1	32	attach	isolated	178:185	arg2	-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one					127:172	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one	89:172	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one	89:172	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.)					
30458190	1	32	attach	isolated	178:185	arg1	part					250:253	the aerial part	239:253	the aerial part of Silene wolgensis	239:273	20-Hydroxyecdysterone - (2β,3β,5β,22R)-2,3,14,20,22,25-hexahydroxycholest-7-en-6-one was isolated in satisfactory yield using ethanol extraction from the aerial part of Silene wolgensis (Hornem.)					
30458190	0	33	theme	structural	20:29	arg1	study					31:35	Bioavailability and structural study	0:35	Bioavailability and structural study of 20-hydroxyecdysone	0:57	Bioavailability and structural study of 20-hydroxyecdysone complexes with cyclodextrins.					
30508655	7	0	theme	extracellular	1147:1159	arg1	ECM					1169:1171	ECM	1169:1171	ECM	1169:1171	One of the main extracellular matrix (ECM) molecules upregulated during regeneration is hyaluronic acid (HA).					
30508655	7	0	theme	extracellular	1147:1159	arg1	matrix					1161:1166	extracellular matrix	1147:1166	the main extracellular matrix (ECM) molecules upregulated during regeneration	1138:1214	One of the main extracellular matrix (ECM) molecules upregulated during regeneration is hyaluronic acid (HA).					
30508655	11	1	theme	transcription	1922:1934	arg1	factors					1936:1942	relevant transcription factors	1913:1942	relevant transcription factors	1913:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	15	2	theme	future	2577:2582	arg1	studies					2592:2598	future in vivo studies	2577:2598	future in vivo studies	2577:2598	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	8	3	theme	diacrylate	1296:1305	arg1	hydrogels					1307:1315	thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels	1252:1315	thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels	1252:1315	Therefore, thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels were generated and functionalized with peptides based on ECM known to influence regeneration, including fibronectin, laminin and tenascin-C.					
30508655	8	4	theme	thiol-modified	1252:1265	arg1	hydrogels					1307:1315	thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels	1252:1315	thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels	1252:1315	Therefore, thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels were generated and functionalized with peptides based on ECM known to influence regeneration, including fibronectin, laminin and tenascin-C.					
30508655	10	5	theme	myogenic	1592:1599	arg1	precursor					1601:1609	myogenic precursor and connective tissue cell proliferation	1592:1650	precursor	1601:1609	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	5	6	theme	cell	853:856	arg1	activation					858:867	satellite cell activation	843:867	satellite cell activation	843:867	Scaffolds that only address structural support are not sufficient to restore functionality and instead need to be designed to both promote satellite cell activation and prevent excessive fibroblast recruitment.					
30508655	12	7	theme	acid	2022:2025	arg1	hydrogels					2032:2040	hyaluronic acid (HA) hydrogels	2011:2040	hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes	2011:2150	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	0	8	link	ECM-derived	52:62	arg1	peptides					64:71	ECM-derived peptides	52:71	ECM-derived peptides to control myoblast behavior	52:100	Functionalization of hyaluronic acid hydrogels with ECM-derived peptides to control myoblast behavior.					
30508655	11	9	theme	cell	1844:1847	arg1	increase					1901:1908	an increase	1898:1908	an increase in relevant transcription factors	1898:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	11	9	theme	cell	1844:1847	arg1	migration					1869:1877	migration	1869:1877	migration	1869:1877	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	11	9	theme	cell	1844:1847	arg1	behaviors					1849:1857	myogenic cell behaviors	1835:1857	myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors	1835:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	11	9	theme	cell	1844:1847	arg1	proliferation					1880:1892	proliferation	1880:1892	proliferation	1880:1892	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	15	10	theme	myoblast	2634:2641	arg1	spreading					2678:2686	spreading	2678:2686	spreading	2678:2686	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	10	theme	myoblast	2634:2641	arg1	migration					2698:2706	enhanced migration	2689:2706	enhanced migration over connective tissue cells	2689:2735	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	10	theme	myoblast	2634:2641	arg1	attachment					2663:2672	attachment	2663:2672	attachment	2663:2672	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	10	theme	myoblast	2634:2641	arg1	proliferation					2648:2660	myoblast cell proliferation	2634:2660	myoblast cell proliferation	2634:2660	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	10	11	theme	tissue	1626:1631	arg1	proliferation					1638:1650	myogenic precursor and connective tissue cell proliferation	1592:1650	proliferation	1638:1650	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	13	12	theme	skeletal	2178:2185	arg1	muscle					2187:2192	skeletal muscle	2178:2192	skeletal muscle	2178:2192	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	2	13	theme	muscle	272:277	arg1	functionality					279:291	muscle functionality	272:291	muscle functionality	272:291	Typically, fibrotic tissue fills the void, which reduces muscle functionality and limb movement.					
30508655	13	14	theme	skeletal	2360:2367	arg1	cells					2384:2388	skeletal muscle-derived cells	2360:2388	skeletal muscle-derived cells	2360:2388	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	10	15	theme	gene	1667:1670	arg1	expression					1672:1681	gene expression	1667:1681	gene expression	1667:1681	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	7	16	theme	molecules	1174:1182	arg1	One					1131:1133	One	1131:1133	One	1131:1133	One of the main extracellular matrix (ECM) molecules upregulated during regeneration is hyaluronic acid (HA).					
30508655	7	16	theme	molecules	1174:1182	arg1	molecules					1174:1182	the main extracellular matrix (ECM) molecules	1138:1182	the main extracellular matrix (ECM) molecules upregulated during regeneration	1138:1214	One of the main extracellular matrix (ECM) molecules upregulated during regeneration is hyaluronic acid (HA).					
30508655	15	17	theme	laminin	2525:2531	arg1	IKVAV					2542:2546	IKVAV	2542:2546	IKVAV	2542:2546	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	17	theme	laminin	2525:2531	arg1	peptide					2533:2539	the laminin peptide	2521:2539	the laminin peptide	2521:2539	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	13	18	theme	cells	2384:2388	arg1	context					2326:2332	the context	2322:2332	the context of HA-based scaffolds and skeletal muscle-derived cells	2322:2388	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	4	19	theme	environment	691:701	arg1	composition					660:670	composition	660:670	composition	660:670	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	4	19	theme	environment	691:701	arg1	stiffness					646:654	stiffness	646:654	stiffness	646:654	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	12	20	with	hydrogels	2032:2040	arg1	stiffness					2059:2067	stiffness	2059:2067	stiffness	2059:2067	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	12	20	with	hydrogels	2032:2040	arg1	peptide					2047:2053	peptide	2047:2053	peptide	2047:2053	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	13	21	theme	scaffolds	2346:2354	arg1	context					2326:2332	the context	2322:2332	the context of HA-based scaffolds and skeletal muscle-derived cells	2322:2388	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	2	22	theme	fibrotic	226:233	arg1	tissue					235:240	fibrotic tissue	226:240	fibrotic tissue	226:240	Typically, fibrotic tissue fills the void, which reduces muscle functionality and limb movement.					
30508655	15	23	theme	3	2495:2495	arg1	%					2496:2496	%	2496:2496	%	2496:2496	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	5	24	theme	excessive	881:889	arg1	recruitment					902:912	excessive fibroblast recruitment	881:912	excessive fibroblast recruitment	881:912	Scaffolds that only address structural support are not sufficient to restore functionality and instead need to be designed to both promote satellite cell activation and prevent excessive fibroblast recruitment.					
30508655	13	25	link	muscle-derived	2369:2382	arg1	cells					2384:2388	skeletal muscle-derived cells	2360:2388	skeletal muscle-derived cells	2360:2388	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	11	26	theme	enhanced	1813:1820	arg1	promotion					1822:1830	the enhanced promotion	1809:1830	the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors	1809:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	0	27	theme	myoblast	84:91	arg1	behavior					93:100	control myoblast behavior	76:100	control myoblast behavior	76:100	Functionalization of hyaluronic acid hydrogels with ECM-derived peptides to control myoblast behavior.					
30508655	3	28	dep	functionality	509:521	arg1	VML					529:531	VML	529:531	VML	529:531	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30508655	6	29	theme	regenerative	986:997	arg1	environment					999:1009	the regenerative environment	982:1009	the regenerative environment	982:1009	The objective of this study was to design a scaffold that mimicked the regenerative environment and determine how the biomechanical properties differentially influence myogenic precursor and connective tissue cells.					
30508655	14	30	from	variations	2425:2434	arg1	mechanics					2439:2447	mechanics	2439:2447	mechanics	2439:2447	Notably, the response of cells to variations in mechanics was dependent on ECM coating and lineage.					
30508655	13	31	with	interaction	2163:2173	arg1	hydrogels					2221:2229	RGD-functionalized HA hydrogels	2199:2229	RGD-functionalized HA hydrogels	2199:2229	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	3	32	theme	wide	323:326	arg1	scaffolds					361:369	natural and synthetic scaffolds	339:369	natural and synthetic scaffolds	339:369	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30508655	3	32	theme	wide	323:326	arg1	variety					328:334	a wide variety	321:334	a wide variety of natural and synthetic scaffolds	321:369	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30508655	5	33	theme	structural	732:741	arg1	support					743:749	structural support	732:749	structural support	732:749	Scaffolds that only address structural support are not sufficient to restore functionality and instead need to be designed to both promote satellite cell activation and prevent excessive fibroblast recruitment.					
30508655	3	34	theme	scaffolds	361:369	arg1	scaffolds					361:369	natural and synthetic scaffolds	339:369	natural and synthetic scaffolds	339:369	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30508655	3	34	theme	scaffolds	361:369	arg1	variety					328:334	a wide variety	321:334	a wide variety of natural and synthetic scaffolds	321:369	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30508655	15	35	theme	tissue	2724:2729	arg1	cells					2731:2735	connective tissue cells	2713:2735	connective tissue cells	2713:2735	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	36	theme	satellite	2801:2809	arg1	cells					2811:2815	activated satellite cells	2791:2815	activated satellite cells	2791:2815	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	12	37	theme	SIGNIFICANCE	1958:1969	arg1	STATEMENT					1945:1953	STATEMENT	1945:1953	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.	1945:2151	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	1	38	theme	Volumetric	103:112	arg1	VML					127:129	VML	127:129	VML	127:129	Volumetric muscle loss (VML) occurs when skeletal muscle injury is too large for the body to fully self-repair.					
30508655	1	38	theme	Volumetric	103:112	arg1	loss					121:124	Volumetric muscle loss	103:124	Volumetric muscle loss (VML)	103:130	Volumetric muscle loss (VML) occurs when skeletal muscle injury is too large for the body to fully self-repair.					
30508655	6	39	theme	myogenic	1083:1090	arg1	precursor					1092:1100	myogenic precursor and connective tissue cells	1083:1128	precursor	1092:1100	The objective of this study was to design a scaffold that mimicked the regenerative environment and determine how the biomechanical properties differentially influence myogenic precursor and connective tissue cells.					
30508655	4	40	theme	Satellite	534:542	arg1	cells					544:548	Satellite cells	534:548	Satellite cells	534:548	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	4	40	theme	Satellite	534:542	arg1	cells					562:566	adult stem cells	551:566	adult stem cells within the muscle capable of restoring smaller injuries	551:622	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	12	41	dep	peptide	2047:2053	arg1	combinations					2069:2080	combinations	2069:2080	combinations	2069:2080	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	0	42	theme	hyaluronic	21:30	arg1	acid					32:35	hyaluronic acid	21:35	hyaluronic acid	21:35	Functionalization of hyaluronic acid hydrogels with ECM-derived peptides to control myoblast behavior.					
30508655	12	43	theme	regenerating	2128:2139	arg1	phenotypes					2141:2150	regenerating phenotypes	2128:2150	regenerating phenotypes	2128:2150	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	3	44	theme	appropriate	423:433	arg1	support					446:452	the appropriate structural support	419:452	the appropriate structural support	419:452	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30508655	6	45	theme	biomechanical	1033:1045	arg1	properties					1047:1056	the biomechanical properties	1029:1056	the biomechanical properties	1029:1056	The objective of this study was to design a scaffold that mimicked the regenerative environment and determine how the biomechanical properties differentially influence myogenic precursor and connective tissue cells.					
30508655	10	46	theme	stiffness	1549:1557	arg1	influence					1533:1541	The influence	1529:1541	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression	1529:1681	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	0	47	theme	ECM-derived	52:62	arg1	peptides					64:71	ECM-derived peptides	52:71	ECM-derived peptides to control myoblast behavior	52:100	Functionalization of hyaluronic acid hydrogels with ECM-derived peptides to control myoblast behavior.					
30508655	13	48	theme	peptides	2272:2279	arg1	none					2254:2257	none	2254:2257	none of the other peptides described in this study	2254:2303	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	7	49	theme	hyaluronic	1219:1228	arg1	acid					1230:1233	hyaluronic acid	1219:1233	hyaluronic acid (HA)	1219:1238	One of the main extracellular matrix (ECM) molecules upregulated during regeneration is hyaluronic acid (HA).					
30508655	7	49	theme	hyaluronic	1219:1228	arg1	HA					1236:1237	HA	1236:1237	HA	1236:1237	One of the main extracellular matrix (ECM) molecules upregulated during regeneration is hyaluronic acid (HA).					
30508655	9	50	with	Scaffolds	1458:1466	arg1	stiffness					1483:1491	different stiffness	1473:1491	different stiffness	1473:1491	Scaffolds with different stiffness were created by varying HA content.					
30508655	1	51	theme	skeletal	144:151	arg1	injury					160:165	skeletal muscle injury	144:165	skeletal muscle injury	144:165	Volumetric muscle loss (VML) occurs when skeletal muscle injury is too large for the body to fully self-repair.					
30508655	14	52	theme	ECM	2466:2468	arg1	coating					2470:2476	ECM coating	2466:2476	ECM coating	2466:2476	Notably, the response of cells to variations in mechanics was dependent on ECM coating and lineage.					
30508655	10	53	theme	peptide	1563:1569	arg1	functionalization					1571:1587	peptide functionalization	1563:1587	peptide functionalization	1563:1587	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	7	54	theme	main	1142:1145	arg1	molecules					1174:1182	the main extracellular matrix (ECM) molecules	1138:1182	the main extracellular matrix (ECM) molecules upregulated during regeneration	1138:1214	One of the main extracellular matrix (ECM) molecules upregulated during regeneration is hyaluronic acid (HA).					
30508655	11	55	theme	relevant	1913:1920	arg1	factors					1936:1942	relevant transcription factors	1913:1942	relevant transcription factors	1913:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	13	56	theme	HA	2218:2219	arg1	hydrogels					2221:2229	RGD-functionalized HA hydrogels	2199:2229	RGD-functionalized HA hydrogels	2199:2229	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	11	57	from	migration	1869:1877	arg1	factors					1936:1942	relevant transcription factors	1913:1942	relevant transcription factors	1913:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	6	58	theme	tissue	1117:1122	arg1	cells					1124:1128	myogenic precursor and connective tissue cells	1083:1128	cells	1124:1128	The objective of this study was to design a scaffold that mimicked the regenerative environment and determine how the biomechanical properties differentially influence myogenic precursor and connective tissue cells.					
30508655	4	59	theme	stem	557:560	arg1	cells					544:548	Satellite cells	534:548	Satellite cells	534:548	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	4	59	theme	stem	557:560	arg1	cells					562:566	adult stem cells	551:566	adult stem cells within the muscle capable of restoring smaller injuries	551:622	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	10	60	theme	connective	1615:1624	arg1	proliferation					1638:1650	myogenic precursor and connective tissue cell proliferation	1592:1650	proliferation	1638:1650	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	15	61	dep	in	2584:2585	arg1	vivo					2587:2590	vivo	2587:2590	vivo	2587:2590	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	62	theme	in	2584:2585	arg1	studies					2592:2598	future in vivo studies	2577:2598	future in vivo studies	2577:2598	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	11	63	from	proliferation	1880:1892	arg1	factors					1936:1942	relevant transcription factors	1913:1942	relevant transcription factors	1913:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	13	64	used	used	2314:2317	arg2	none					2254:2257	none	2254:2257	none of the other peptides described in this study	2254:2303	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	5	65	theme	fibroblast	891:900	arg1	recruitment					902:912	excessive fibroblast recruitment	881:912	excessive fibroblast recruitment	881:912	Scaffolds that only address structural support are not sufficient to restore functionality and instead need to be designed to both promote satellite cell activation and prevent excessive fibroblast recruitment.					
30508655	11	66	theme	HA	1726:1727	arg1	hydrogels					1729:1737	HA hydrogels	1726:1737	HA hydrogels functionalized with the laminin peptide, IKVAV,	1726:1785	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	5	67	theme	satellite	843:851	arg1	activation					858:867	satellite cell activation	843:867	satellite cell activation	843:867	Scaffolds that only address structural support are not sufficient to restore functionality and instead need to be designed to both promote satellite cell activation and prevent excessive fibroblast recruitment.					
30508655	15	68	theme	connective	2713:2722	arg1	cells					2731:2735	connective tissue cells	2713:2735	connective tissue cells	2713:2735	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	14	69	theme	cells	2416:2420	arg1	response					2404:2411	the response	2400:2411	the response of cells to variations in mechanics	2400:2447	Notably, the response of cells to variations in mechanics was dependent on ECM coating and lineage.					
30508655	14	69	theme	cells	2416:2420	arg1	dependent					2453:2461	dependent	2453:2461	dependent	2453:2461	Notably, the response of cells to variations in mechanics was dependent on ECM coating and lineage.					
30508655	2	70	theme	limb	297:300	arg1	movement					302:309	limb movement	297:309	limb movement	297:309	Typically, fibrotic tissue fills the void, which reduces muscle functionality and limb movement.					
30508655	9	71	theme	different	1473:1481	arg1	stiffness					1483:1491	different stiffness	1473:1491	different stiffness	1473:1491	Scaffolds with different stiffness were created by varying HA content.					
30508655	12	72	theme	hyaluronic	2011:2020	arg1	acid					2022:2025	hyaluronic acid	2011:2025	hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes	2011:2150	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	12	72	theme	hyaluronic	2011:2020	arg1	HA					2028:2029	HA	2028:2029	HA	2028:2029	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	8	73	theme	HA	1267:1268	arg1	hydrogels					1307:1315	thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels	1252:1315	thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels	1252:1315	Therefore, thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels were generated and functionalized with peptides based on ECM known to influence regeneration, including fibronectin, laminin and tenascin-C.					
30508655	10	74	theme	cell	1633:1636	arg1	proliferation					1638:1650	myogenic precursor and connective tissue cell proliferation	1592:1650	proliferation	1638:1650	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	11	75	theme	behaviors	1849:1857	arg1	promotion					1822:1830	the enhanced promotion	1809:1830	the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors	1809:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	15	76	theme	cell	2643:2646	arg1	spreading					2678:2686	spreading	2678:2686	spreading	2678:2686	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	76	theme	cell	2643:2646	arg1	migration					2698:2706	enhanced migration	2689:2706	enhanced migration over connective tissue cells	2689:2735	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	76	theme	cell	2643:2646	arg1	attachment					2663:2672	attachment	2663:2672	attachment	2663:2672	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	76	theme	cell	2643:2646	arg1	proliferation					2648:2660	myoblast cell proliferation	2634:2660	myoblast cell proliferation	2634:2660	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	8	77	theme	poly	1274:1277	arg1	hydrogels					1307:1315	thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels	1252:1315	thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels	1252:1315	Therefore, thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels were generated and functionalized with peptides based on ECM known to influence regeneration, including fibronectin, laminin and tenascin-C.					
30508655	11	78	theme	myogenic	1835:1842	arg1	increase					1901:1908	an increase	1898:1908	an increase in relevant transcription factors	1898:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	11	78	theme	myogenic	1835:1842	arg1	migration					1869:1877	migration	1869:1877	migration	1869:1877	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	11	78	theme	myogenic	1835:1842	arg1	behaviors					1849:1857	myogenic cell behaviors	1835:1857	myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors	1835:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	11	78	theme	myogenic	1835:1842	arg1	proliferation					1880:1892	proliferation	1880:1892	proliferation	1880:1892	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	13	79	theme	muscle	2187:2192	arg1	interaction					2163:2173	the interaction	2159:2173	the interaction of skeletal muscle with RGD-functionalized HA hydrogels	2159:2229	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	13	80	theme	muscle-derived	2369:2382	arg1	cells					2384:2388	skeletal muscle-derived cells	2360:2388	skeletal muscle-derived cells	2360:2388	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	12	81	theme	study	1989:1993	arg1	goal					1976:1979	The goal	1972:1979	The goal of this study	1972:1993	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	10	82	from	influence	1533:1541	arg1	proliferation					1638:1650	myogenic precursor and connective tissue cell proliferation	1592:1650	proliferation	1638:1650	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	10	82	from	influence	1533:1541	arg1	precursor					1601:1609	myogenic precursor and connective tissue cell proliferation	1592:1650	precursor	1601:1609	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	10	82	from	influence	1533:1541	arg1	migration					1653:1661	migration	1653:1661	migration	1653:1661	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	10	82	from	influence	1533:1541	arg1	expression					1672:1681	gene expression	1667:1681	gene expression	1667:1681	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	8	83	theme	ethylene	1279:1286	arg1	poly					1274:1277	poly	1274:1277	poly(ethylene glycol)	1274:1294	Therefore, thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels were generated and functionalized with peptides based on ECM known to influence regeneration, including fibronectin, laminin and tenascin-C.					
30508655	8	83	theme	ethylene	1279:1286	arg1	glycol					1288:1293	ethylene glycol	1279:1293	ethylene glycol	1279:1293	Therefore, thiol-modified HA and poly(ethylene glycol) diacrylate hydrogels were generated and functionalized with peptides based on ECM known to influence regeneration, including fibronectin, laminin and tenascin-C.					
30508655	13	84	theme	RGD-functionalized	2199:2216	arg1	hydrogels					2221:2229	RGD-functionalized HA hydrogels	2199:2229	RGD-functionalized HA hydrogels	2199:2229	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	9	85	theme	HA	1517:1518	arg1	content					1520:1526	HA content	1517:1526	HA content	1517:1526	Scaffolds with different stiffness were created by varying HA content.					
30508655	6	86	theme	study	937:941	arg1	objective					919:927	The objective	915:927	The objective of this study	915:941	The objective of this study was to design a scaffold that mimicked the regenerative environment and determine how the biomechanical properties differentially influence myogenic precursor and connective tissue cells.					
30508655	4	87	theme	surrounding	679:689	arg1	environment					691:701	the surrounding environment	675:701	the surrounding environment	675:701	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	13	88	theme	HA-based	2337:2344	arg1	scaffolds					2346:2354	HA-based scaffolds	2337:2354	HA-based scaffolds	2337:2354	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	0	89	theme	control	76:82	arg1	behavior					93:100	control myoblast behavior	76:100	control myoblast behavior	76:100	Functionalization of hyaluronic acid hydrogels with ECM-derived peptides to control myoblast behavior.					
30508655	12	90	dep	STATEMENT	1945:1953	arg1	was					1995:1997	was	1995:1997	was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes	1995:2150	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	15	91	theme	%	2496:2496	arg1	HA					2498:2499	The 3% HA	2491:2499	The 3% HA functionalized with the laminin peptide, IKVAV,	2491:2547	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	1	92	theme	muscle	153:158	arg1	injury					160:165	skeletal muscle injury	144:165	skeletal muscle injury	144:165	Volumetric muscle loss (VML) occurs when skeletal muscle injury is too large for the body to fully self-repair.					
30508655	12	93	theme	muscle-derived	2099:2112	arg1	cells					2114:2118	muscle-derived cells	2099:2118	muscle-derived cells	2099:2118	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	15	94	theme	enhanced	2689:2696	arg1	migration					2698:2706	enhanced migration	2689:2706	enhanced migration over connective tissue cells	2689:2735	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	15	94	theme	enhanced	2689:2696	arg1	proliferation					2648:2660	myoblast cell proliferation	2634:2660	myoblast cell proliferation	2634:2660	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	3	95	theme	natural	339:345	arg1	scaffolds					361:369	natural and synthetic scaffolds	339:369	natural and synthetic scaffolds	339:369	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30508655	4	96	theme	smaller	607:613	arg1	injuries					615:622	smaller injuries	607:622	smaller injuries	607:622	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	6	97	theme	connective	1106:1115	arg1	cells					1124:1128	myogenic precursor and connective tissue cells	1083:1128	cells	1124:1128	The objective of this study was to design a scaffold that mimicked the regenerative environment and determine how the biomechanical properties differentially influence myogenic precursor and connective tissue cells.					
30508655	15	98	theme	activated	2791:2799	arg1	cells					2811:2815	activated satellite cells	2791:2815	activated satellite cells	2791:2815	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	11	99	from	increase	1901:1908	arg1	factors					1936:1942	relevant transcription factors	1913:1942	relevant transcription factors	1913:1942	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	0	100	theme	acid	32:35	arg1	Functionalization					0:16	Functionalization	0:16	Functionalization of hyaluronic acid	0:35	Functionalization of hyaluronic acid hydrogels with ECM-derived peptides to control myoblast behavior.					
30508655	15	101	theme	most	2560:2563	arg1	promise					2565:2571	the most promise	2556:2571	the most promise for future in vivo studies	2556:2598	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	1	102	theme	muscle	114:119	arg1	VML					127:129	VML	127:129	VML	127:129	Volumetric muscle loss (VML) occurs when skeletal muscle injury is too large for the body to fully self-repair.					
30508655	1	102	theme	muscle	114:119	arg1	loss					121:124	Volumetric muscle loss	103:124	Volumetric muscle loss (VML)	103:130	Volumetric muscle loss (VML) occurs when skeletal muscle injury is too large for the body to fully self-repair.					
30508655	3	103	theme	synthetic	351:359	arg1	scaffolds					361:369	natural and synthetic scaffolds	339:369	natural and synthetic scaffolds	339:369	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30508655	4	104	theme	adult	551:555	arg1	cells					544:548	Satellite cells	534:548	Satellite cells	534:548	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	4	104	theme	adult	551:555	arg1	cells					562:566	adult stem cells	551:566	adult stem cells within the muscle capable of restoring smaller injuries	551:622	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	15	105	theme	transcription	2753:2765	arg1	factors					2767:2773	transcription factors	2753:2773	transcription factors associated with activated satellite cells	2753:2815	The 3% HA functionalized with the laminin peptide, IKVAV, showed the most promise for future in vivo studies, as these hydrogels best promoted myoblast cell proliferation, attachment and spreading, enhanced migration over connective tissue cells and upregulated transcription factors associated with activated satellite cells.					
30508655	3	106	theme	structural	435:444	arg1	support					446:452	the appropriate structural support	419:452	the appropriate structural support	419:452	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30508655	7	107	theme	matrix	1161:1166	arg1	molecules					1174:1182	the main extracellular matrix (ECM) molecules	1138:1182	the main extracellular matrix (ECM) molecules upregulated during regeneration	1138:1214	One of the main extracellular matrix (ECM) molecules upregulated during regeneration is hyaluronic acid (HA).					
30508655	13	108	theme	other	2266:2270	arg1	peptides					2272:2279	the other peptides	2262:2279	the other peptides described in this study	2262:2303	While the interaction of skeletal muscle with RGD-functionalized HA hydrogels has been investigated, none of the other peptides described in this study had been used in the context of HA-based scaffolds and skeletal muscle-derived cells.					
30508655	10	109	theme	HA	1546:1547	arg1	stiffness					1549:1557	HA stiffness	1546:1557	HA stiffness	1546:1557	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	12	110	link	muscle-derived	2099:2112	arg1	cells					2114:2118	muscle-derived cells	2099:2118	muscle-derived cells	2099:2118	STATEMENT OF SIGNIFICANCE: The goal of this study was to identify hyaluronic acid (HA) hydrogels with peptide and stiffness combinations that will direct muscle-derived cells towards regenerating phenotypes.					
30508655	4	111	theme	capable	586:592	arg1	muscle					579:584	the muscle	575:584	the muscle capable of restoring smaller injuries	575:622	Satellite cells, adult stem cells within the muscle capable of restoring smaller injuries, are sensitive to the stiffness and composition of the surrounding environment.					
30508655	11	112	theme	laminin	1763:1769	arg1	peptide					1771:1777	the laminin peptide	1759:1777	the laminin peptide	1759:1777	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	11	112	theme	laminin	1763:1769	arg1	IKVAV					1780:1784	IKVAV	1780:1784	IKVAV	1780:1784	Our results indicated that HA hydrogels functionalized with the laminin peptide, IKVAV, show potential due to the enhanced promotion of myogenic cell behaviors including migration, proliferation and an increase in relevant transcription factors.					
30508655	10	113	theme	functionalization	1571:1587	arg1	influence					1533:1541	The influence	1529:1541	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression	1529:1681	The influence of HA stiffness and peptide functionalization on myogenic precursor and connective tissue cell proliferation, migration and gene expression was quantified.					
30508655	3	114	theme	muscle	502:507	arg1	functionality					509:521	muscle functionality	502:521	muscle functionality	502:521	Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML.					
30268514	0	0	theme	cranial	83:89	arg1	defects					91:97	cranial defects	83:97	cranial defects in rats	83:105	Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats.					
30268514	0	1	with	mineralized	33:43	arg1	hydroxyapatite					50:63	hydroxyapatite	50:63	hydroxyapatite	50:63	Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats.					
30268514	1	2	theme	clinical	146:153	arg1	challenges					155:164	several clinical challenges	138:164	several clinical challenges that involve extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts	138:280	In traumatology, we encounter several clinical challenges that involve extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts.					
30268514	13	3	theme	sponges	1870:1876	arg1	Mineralization					1852:1865	Mineralization	1852:1865	Mineralization of sponges	1852:1876	Mineralization of sponges did not stimulate bone neoformation, with bone repair being incomplete over the experimental period.					
30268514	6	4	theme	polymer	901:907	arg1	blend					909:913	a polymer blend	899:913	a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite	899:992	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	5	5	theme	three-dimensional	648:664	arg1	structure					666:674	a three-dimensional structure	646:674	a three-dimensional structure that permits cell adhesion and proliferation, thus improving osteogenesis	646:748	Furthermore, the addition of chitosan contributes to the formation of a three-dimensional structure that permits cell adhesion and proliferation, thus improving osteogenesis.					
30268514	7	6	theme	collagen	1211:1218	arg1	blend					1202:1206	the blend	1198:1206	the blend of collagen and chitosan	1198:1231	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	10	7	theme	formation	1556:1564	arg1	evaluation					1533:1542	histological evaluation	1520:1542	histological evaluation of new bone formation at the surgical site	1520:1585	The samples were processed for histological evaluation of new bone formation at the surgical site.					
30268514	13	8	theme	bone	1920:1923	arg1	repair					1925:1930	bone repair	1920:1930	bone repair	1920:1930	Mineralization of sponges did not stimulate bone neoformation, with bone repair being incomplete over the experimental period.					
30268514	5	9	theme	structure	666:674	arg1	formation					633:641	the formation	629:641	the formation of a three-dimensional structure that permits cell adhesion and proliferation, thus improving osteogenesis	629:748	Furthermore, the addition of chitosan contributes to the formation of a three-dimensional structure that permits cell adhesion and proliferation, thus improving osteogenesis.					
30268514	5	10	theme	chitosan	605:612	arg1	addition					593:600	the addition	589:600	the addition of chitosan	589:612	Furthermore, the addition of chitosan contributes to the formation of a three-dimensional structure that permits cell adhesion and proliferation, thus improving osteogenesis.					
30268514	11	11	theme	Macroscopic	1588:1598	arg1	analysis					1617:1624	Macroscopic and radiographic analysis	1588:1624	Macroscopic and radiographic analysis	1588:1624	Macroscopic and radiographic analysis demonstrated the biocompatibility of the blends.					
30268514	0	12	theme	defects	91:97	arg1	repair					73:78	the repair	69:78	the repair of cranial defects in rats	69:105	Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats.					
30268514	11	13	theme	blends	1667:1672	arg1	biocompatibility					1643:1658	the biocompatibility	1639:1658	the biocompatibility of the blends	1639:1672	Macroscopic and radiographic analysis demonstrated the biocompatibility of the blends.					
30268514	1	14	from	encounter	128:136	arg1	traumatology					111:122	traumatology	111:122	traumatology	111:122	In traumatology, we encounter several clinical challenges that involve extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts.					
30268514	12	15	with	continuity	1729:1738	arg1	edges					1749:1753	the edges	1745:1753	the edges of the defect	1745:1767	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	15	16	theme	short	2280:2284	arg1	period					2286:2291	a short period	2278:2291	a short period of time	2278:2299	However, the collagen/chitosan sponges used in this study are suitable to stimulate osteogenesis in cranial defects, although this process is slow and not sufficient to achieve complete bone regeneration over a short period of time.					
30268514	7	17	theme	biomaterial	1154:1164	arg1	implantation					1166:1177	biomaterial implantation	1154:1177	biomaterial implantation	1154:1177	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	7	18	from	bone	1070:1073	arg1	creation					1028:1035	the creation	1024:1035	the creation of a defect in the left parietal bone	1024:1073	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	7	19	dep	used	1015:1018	arg1	group					1140:1144	a control group	1130:1144	a control group without biomaterial implantation	1130:1177	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	7	19	dep	used	1015:1018	arg1	group					1182:1186	a group	1180:1186	a group receiving the blend of collagen and chitosan	1180:1231	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	7	19	dep	used	1015:1018	arg1	group					1240:1244	a group	1238:1244	a group receiving this blend combined with hydroxyapatite	1238:1294	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	3	20	theme	bone	423:426	arg1	growth					409:414	the growth	405:414	the growth of new bone and vascular tissue	405:446	These polymers provide a suitable environment for the growth of new bone and vascular tissue, which are essential for repair.					
30268514	15	21	from	osteogenesis	2153:2164	arg1	defects					2177:2183	cranial defects	2169:2183	cranial defects	2169:2183	However, the collagen/chitosan sponges used in this study are suitable to stimulate osteogenesis in cranial defects, although this process is slow and not sufficient to achieve complete bone regeneration over a short period of time.					
30268514	10	22	theme	surgical	1573:1580	arg1	site					1582:1585	the surgical site	1569:1585	the surgical site	1569:1585	The samples were processed for histological evaluation of new bone formation at the surgical site.					
30268514	10	23	theme	new	1547:1549	arg1	formation					1556:1564	new bone formation	1547:1564	new bone formation	1547:1564	The samples were processed for histological evaluation of new bone formation at the surgical site.					
30268514	6	24	theme	rats	877:880	arg1	skull					868:872	the skull	864:872	the skull of rats	864:880	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	10	25	theme	bone	1551:1554	arg1	formation					1556:1564	new bone formation	1547:1564	new bone formation	1547:1564	The samples were processed for histological evaluation of new bone formation at the surgical site.					
30268514	6	26	theme	bone	789:792	arg1	formation					794:802	bone formation	789:802	bone formation	789:802	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	12	27	from	groups	1824:1829	arg1	observation					1779:1789	the observation	1775:1789	the observation of higher volumes in the grafted groups compared to control	1775:1849	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	7	28	theme	defect	1042:1047	arg1	creation					1028:1035	the creation	1024:1035	the creation of a defect in the left parietal bone	1024:1073	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	1	29	theme	extensive	179:187	arg1	loss					194:197	extensive bone loss	179:197	extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts	179:280	In traumatology, we encounter several clinical challenges that involve extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts.					
30268514	3	30	theme	suitable	380:387	arg1	essential					459:467	essential	459:467	essential	459:467	These polymers provide a suitable environment for the growth of new bone and vascular tissue, which are essential for repair.					
30268514	3	30	theme	suitable	380:387	arg1	environment					389:399	a suitable environment	378:399	a suitable environment	378:399	These polymers provide a suitable environment for the growth of new bone and vascular tissue, which are essential for repair.					
30268514	1	31	theme	autologous	264:273	arg1	grafts					275:280	autologous grafts	264:280	autologous grafts	264:280	In traumatology, we encounter several clinical challenges that involve extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts.					
30268514	6	32	theme	bone	825:828	arg1	defects					830:836	bone defects	825:836	bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite	825:992	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	15	33	theme	cranial	2169:2175	arg1	defects					2177:2183	cranial defects	2169:2183	cranial defects	2169:2183	However, the collagen/chitosan sponges used in this study are suitable to stimulate osteogenesis in cranial defects, although this process is slow and not sufficient to achieve complete bone regeneration over a short period of time.					
30268514	3	34	theme	new	419:421	arg1	bone					423:426	new bone	419:426	new bone	419:426	These polymers provide a suitable environment for the growth of new bone and vascular tissue, which are essential for repair.					
30268514	0	35	theme	sponges	25:31	arg1	Use					0:2	Use	0:2	Use of collagen/chitosan sponges	0:31	Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats.					
30268514	7	36	theme	left	1056:1059	arg1	bone					1070:1073	the left parietal bone	1052:1073	the left parietal bone	1052:1073	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	13	37	theme	experimental	1958:1969	arg1	period					1971:1976	the experimental period	1954:1976	the experimental period	1954:1976	Mineralization of sponges did not stimulate bone neoformation, with bone repair being incomplete over the experimental period.					
30268514	0	38	theme	collagen/chitosan	7:23	arg1	sponges					25:31	collagen/chitosan sponges	7:31	collagen/chitosan sponges	7:31	Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats.					
30268514	15	39	theme	complete	2246:2253	arg1	regeneration					2260:2271	complete bone regeneration	2246:2271	complete bone regeneration	2246:2271	However, the collagen/chitosan sponges used in this study are suitable to stimulate osteogenesis in cranial defects, although this process is slow and not sufficient to achieve complete bone regeneration over a short period of time.					
30268514	7	40	theme	parietal	1061:1068	arg1	bone					1070:1073	the left parietal bone	1052:1073	the left parietal bone	1052:1073	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	2	41	theme	good	285:288	arg1	alternative					290:300	A good alternative	283:300	A good alternative	283:300	A good alternative is the use of synthetic biomaterials as substitutes.					
30268514	2	41	theme	good	285:288	arg1	use					309:311	the use	305:311	the use of synthetic biomaterials as substitutes	305:352	A good alternative is the use of synthetic biomaterials as substitutes.					
30268514	12	42	from	observation	1779:1789	arg1	groups					1824:1829	the grafted groups	1812:1829	the grafted groups	1812:1829	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	15	43	theme	bone	2255:2258	arg1	regeneration					2260:2271	complete bone regeneration	2246:2271	complete bone regeneration	2246:2271	However, the collagen/chitosan sponges used in this study are suitable to stimulate osteogenesis in cranial defects, although this process is slow and not sufficient to achieve complete bone regeneration over a short period of time.					
30268514	12	44	from	volumes	1801:1807	arg1	groups					1824:1829	the grafted groups	1812:1829	the grafted groups	1812:1829	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	12	45	theme	defect	1762:1767	arg1	edges					1749:1753	the edges	1745:1753	the edges of the defect	1745:1767	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	3	46	theme	vascular	432:439	arg1	tissue					441:446	vascular tissue	432:446	vascular tissue	432:446	These polymers provide a suitable environment for the growth of new bone and vascular tissue, which are essential for repair.					
30268514	1	47	theme	bone	189:192	arg1	loss					194:197	extensive bone loss	179:197	extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts	179:280	In traumatology, we encounter several clinical challenges that involve extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts.					
30268514	1	48	theme	related	209:215	arg1	loss					194:197	extensive bone loss	179:197	extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts	179:280	In traumatology, we encounter several clinical challenges that involve extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts.					
30268514	12	49	theme	new	1708:1710	arg1	bone					1712:1715	new bone	1708:1715	new bone	1708:1715	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	0	50	from	repair	73:78	arg1	rats					102:105	rats	102:105	rats	102:105	Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats.					
30268514	7	51	dep	groups	1103:1108	arg1	each					1124:1127	each	1124:1127	each	1124:1127	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	12	52	theme	higher	1794:1799	arg1	volumes					1801:1807	higher volumes	1794:1807	higher volumes in the grafted groups	1794:1829	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	3	53	theme	tissue	441:446	arg1	growth					409:414	the growth	405:414	the growth of new bone and vascular tissue	405:446	These polymers provide a suitable environment for the growth of new bone and vascular tissue, which are essential for repair.					
30268514	6	54	theme	tendon	936:941	arg1	collagen					943:950	bovine tendon collagen	929:950	bovine tendon collagen	929:950	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	12	55	theme	grafted	1816:1822	arg1	groups					1824:1829	the grafted groups	1812:1829	the grafted groups	1812:1829	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	6	56	theme	study	767:771	arg1	aim					755:757	The aim	751:757	The aim of this study	751:771	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	2	57	theme	biomaterials	326:337	arg1	alternative					290:300	A good alternative	283:300	A good alternative	283:300	A good alternative is the use of synthetic biomaterials as substitutes.					
30268514	2	57	theme	biomaterials	326:337	arg1	use					309:311	the use	305:311	the use of synthetic biomaterials as substitutes	305:352	A good alternative is the use of synthetic biomaterials as substitutes.					
30268514	7	58	theme	animals	1116:1122	arg1	groups					1103:1108	three groups	1097:1108	three groups of 10 animals each	1097:1127	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	7	58	theme	animals	1116:1122	arg1	animals					1116:1122	10 animals	1113:1122	10 animals	1113:1122	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	6	59	theme	bovine	929:934	arg1	collagen					943:950	bovine tendon collagen	929:950	bovine tendon collagen	929:950	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	5	60	theme	cell	689:692	arg1	adhesion					694:701	cell adhesion	689:701	cell adhesion	689:701	Furthermore, the addition of chitosan contributes to the formation of a three-dimensional structure that permits cell adhesion and proliferation, thus improving osteogenesis.					
30268514	6	61	dep	defects	830:836	arg1	grafted					886:892	grafted	886:892	grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite	886:992	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	6	61	dep	defects	830:836	arg1	induced					853:859	induced	853:859	induced in the skull of rats	853:880	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	2	62	theme	synthetic	316:324	arg1	biomaterials					326:337	synthetic biomaterials	316:337	synthetic biomaterials	316:337	A good alternative is the use of synthetic biomaterials as substitutes.					
30268514	7	63	used	used	1015:1018	arg2	animals					1002:1008	Thirty animals	995:1008	Thirty animals	995:1008	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	0	64	from	rats	102:105	arg1	repair					73:78	the repair	69:78	the repair of cranial defects in rats	69:105	Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats.					
30268514	12	65	theme	volumes	1801:1807	arg1	observation					1779:1789	the observation	1775:1789	the observation of higher volumes in the grafted groups compared to control	1775:1849	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	9	66	theme	radiographic	1463:1474	arg1	examination					1476:1486	radiographic examination	1463:1486	radiographic examination	1463:1486	After sacrifice, the skulls were removed for macroscopic photodocumentation and radiographic examination.					
30268514	13	67	theme	bone	1896:1899	arg1	neoformation					1901:1912	bone neoformation	1896:1912	bone neoformation	1896:1912	Mineralization of sponges did not stimulate bone neoformation, with bone repair being incomplete over the experimental period.					
30268514	7	68	from	creation	1028:1035	arg1	bone					1070:1073	the left parietal bone	1052:1073	the left parietal bone	1052:1073	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	6	69	theme	defects	830:836	arg1	repair					815:820	the repair	811:820	the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite	811:992	The aim of this study was to evaluate bone formation during the repair of bone defects experimentally induced in the skull of rats and grafted with a polymer blend consisting of bovine tendon collagen and chitosan combined with hydroxyapatite.					
30268514	15	70	theme	collagen/chitosan	2082:2098	arg1	suitable					2131:2138	suitable	2131:2138	suitable	2131:2138	However, the collagen/chitosan sponges used in this study are suitable to stimulate osteogenesis in cranial defects, although this process is slow and not sufficient to achieve complete bone regeneration over a short period of time.					
30268514	15	70	theme	collagen/chitosan	2082:2098	arg1	sponges					2100:2106	the collagen/chitosan sponges	2078:2106	the collagen/chitosan sponges used in this study	2078:2125	However, the collagen/chitosan sponges used in this study are suitable to stimulate osteogenesis in cranial defects, although this process is slow and not sufficient to achieve complete bone regeneration over a short period of time.					
30268514	10	71	from	site	1582:1585	arg1	evaluation					1533:1542	histological evaluation	1520:1542	histological evaluation of new bone formation at the surgical site	1520:1585	The samples were processed for histological evaluation of new bone formation at the surgical site.					
30268514	7	72	theme	chitosan	1224:1231	arg1	blend					1202:1206	the blend	1198:1206	the blend of collagen and chitosan	1198:1231	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	9	73	theme	macroscopic	1428:1438	arg1	photodocumentation					1440:1457	macroscopic photodocumentation	1428:1457	macroscopic photodocumentation	1428:1457	After sacrifice, the skulls were removed for macroscopic photodocumentation and radiographic examination.					
30268514	0	74	from	defects	91:97	arg1	rats					102:105	rats	102:105	rats	102:105	Use of collagen/chitosan sponges mineralized with hydroxyapatite for the repair of cranial defects in rats.					
30268514	11	75	theme	radiographic	1604:1615	arg1	analysis					1617:1624	Macroscopic and radiographic analysis	1588:1624	Macroscopic and radiographic analysis	1588:1624	Macroscopic and radiographic analysis demonstrated the biocompatibility of the blends.					
30268514	8	76	dep	weeks	1362:1366	arg1	surgery					1374:1380	surgery	1374:1380	surgery	1374:1380	Each group was subdivided and the animals were sacrificed 3 or 8 weeks after surgery.					
30268514	12	77	theme	bone	1712:1715	arg1	formation					1695:1703	the formation	1691:1703	the formation of new bone	1691:1715	Histologically, the formation of new bone occurred in continuity with the edges of the defect, with the observation of higher volumes in the grafted groups compared to control.					
30268514	15	78	theme	time	2296:2299	arg1	period					2286:2291	a short period	2278:2291	a short period of time	2278:2299	However, the collagen/chitosan sponges used in this study are suitable to stimulate osteogenesis in cranial defects, although this process is slow and not sufficient to achieve complete bone regeneration over a short period of time.					
30268514	10	79	theme	histological	1520:1531	arg1	evaluation					1533:1542	histological evaluation	1520:1542	histological evaluation of new bone formation at the surgical site	1520:1585	The samples were processed for histological evaluation of new bone formation at the surgical site.					
30268514	4	80	theme	Collagen/hydroxyapatite	481:503	arg1	composites					505:514	Collagen/hydroxyapatite composites	481:514	Collagen/hydroxyapatite composites	481:514	Collagen/hydroxyapatite composites have proven to be biocompatible and to behave mechanically.					
30268514	7	81	theme	control	1132:1138	arg1	group					1140:1144	a control group	1130:1144	a control group without biomaterial implantation	1130:1177	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30268514	14	82	theme	hydroxyapatite	2028:2041	arg1	addition					2016:2023	the addition	2012:2023	the addition of hydroxyapatite	2012:2041	In conclusion, mineralization by the addition of hydroxyapatite should be better studied.					
30268514	1	83	theme	several	138:144	arg1	challenges					155:164	several clinical challenges	138:164	several clinical challenges that involve extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts	138:280	In traumatology, we encounter several clinical challenges that involve extensive bone loss primarily related to trauma, conditions that can be treated with autologous grafts.					
30268514	7	84	from	defect	1042:1047	arg1	bone					1070:1073	the left parietal bone	1052:1073	the left parietal bone	1052:1073	Thirty animals were used for the creation of a defect in the left parietal bone and were divided into three groups of 10 animals each: a control group without biomaterial implantation, a group receiving the blend of collagen and chitosan, and a group receiving this blend combined with hydroxyapatite.					
30142254	9	0	theme	crystal	1255:1261	arg1	polymorphs					1263:1272	crystal polymorphs	1255:1272	crystal polymorphs	1255:1272	Furthermore, the extracted proteins from the cusps of radula or the framework chitin induce packing of crystals and possibly affect crystal polymorphs in vitro.					
30142254	10	1	theme	biomimetic	1415:1424	arg1	strategies					1426:1435	biomimetic strategies	1415:1435	biomimetic strategies aimed at designing hard materials at room temperature	1415:1489	This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.					
30142254	9	2	theme	extracted	1140:1148	arg1	proteins					1150:1157	the extracted proteins	1136:1157	the extracted proteins from the cusps of radula or the framework chitin	1136:1206	Furthermore, the extracted proteins from the cusps of radula or the framework chitin induce packing of crystals and possibly affect crystal polymorphs in vitro.					
30142254	1	3	theme	hardest	139:145	arg1	teeth					121:125	mineralized teeth	109:125	mineralized teeth	109:125	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	3	theme	hardest	139:145	arg1	strongest					151:159	strongest	151:159	strongest	151:159	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	3	theme	hardest	139:145	arg1	one					128:130	one	128:130	one	128:130	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	3	theme	hardest	139:145	arg1	hardest					139:145	hardest	139:145	hardest	139:145	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	5	4	theme	leading	700:706	arg1	regions					708:714	leading regions	700:714	leading regions	700:714	Small nanoneedles are densely packed at the tips or leading regions of the cusps.					
30142254	9	5	theme	radula	1177:1182	arg1	cusps					1168:1172	the cusps	1164:1172	the cusps of radula or the framework chitin	1164:1206	Furthermore, the extracted proteins from the cusps of radula or the framework chitin induce packing of crystals and possibly affect crystal polymorphs in vitro.					
30142254	3	6	from	proteomics	489:498	arg1	teeth					528:532	the teeth	524:532	the teeth of limpet Cellana toreuma	524:558	The study investigates the microstructure, proteomics, and crystallization in the teeth of limpet Cellana toreuma.					
30142254	10	7	theme	unique	1321:1326	arg1	process					1346:1352	the unique biomineralization process	1317:1352	the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature	1317:1489	This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.					
30142254	2	8	theme	chemical	275:282	arg1	composition					284:294	chemical composition	275:294	chemical composition	275:294	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	5	9	theme	Small	648:652	arg1	nanoneedles					654:664	Small nanoneedles	648:664	Small nanoneedles	648:664	Small nanoneedles are densely packed at the tips or leading regions of the cusps.					
30142254	6	10	theme	synthesized	781:791	arg1	goethite					793:800	chemically synthesized goethite	770:800	chemically synthesized goethite	770:800	In contrast, big nanoneedles resembling chemically synthesized goethite are loosely packed in the trailing regions of the cusps.					
30142254	2	11	theme	teeth	303:307	arg1	ultrastructure					256:269	ultrastructure	256:269	ultrastructure	256:269	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	2	11	theme	teeth	303:307	arg1	composition					284:294	chemical composition	275:294	chemical composition	275:294	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	8	12	theme	goethite-binding	1010:1025	arg1	experiment					1027:1036	A goethite-binding experiment	1008:1036	A goethite-binding experiment coupled with proteomics and RNA-seq	1008:1072	A goethite-binding experiment coupled with proteomics and RNA-seq highlights six chitin-binding proteins (CtCBPs).					
30142254	3	13	dep	microstructure	473:486	arg1	the					469:471	the	469:471	the	469:471	The study investigates the microstructure, proteomics, and crystallization in the teeth of limpet Cellana toreuma.					
30142254	1	14	theme	strongest	151:159	arg1	teeth					121:125	mineralized teeth	109:125	mineralized teeth	109:125	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	14	theme	strongest	151:159	arg1	strongest					151:159	strongest	151:159	strongest	151:159	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	14	theme	strongest	151:159	arg1	one					128:130	one	128:130	one	128:130	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	14	theme	strongest	151:159	arg1	hardest					139:145	hardest	139:145	hardest	139:145	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	2	15	theme	fundamental	407:417	arg1	process					437:443	this unique and fundamental biomineralization process	391:443	this unique and fundamental biomineralization process	391:443	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	6	16	theme	cusps	852:856	arg1	regions					837:843	the trailing regions	824:843	the trailing regions of the cusps	824:856	In contrast, big nanoneedles resembling chemically synthesized goethite are loosely packed in the trailing regions of the cusps.					
30142254	5	17	theme	cusps	723:727	arg1	tips					692:695	the tips	688:695	the tips	688:695	Small nanoneedles are densely packed at the tips or leading regions of the cusps.					
30142254	5	17	theme	cusps	723:727	arg1	regions					708:714	leading regions	700:714	leading regions	700:714	Small nanoneedles are densely packed at the tips or leading regions of the cusps.					
30142254	3	18	theme	Cellana	544:550	arg1	toreuma					552:558	limpet Cellana toreuma	537:558	limpet Cellana toreuma	537:558	The study investigates the microstructure, proteomics, and crystallization in the teeth of limpet Cellana toreuma.					
30142254	6	19	theme	big	743:745	arg1	nanoneedles					747:757	big nanoneedles	743:757	big nanoneedles resembling chemically synthesized goethite	743:800	In contrast, big nanoneedles resembling chemically synthesized goethite are loosely packed in the trailing regions of the cusps.					
30142254	3	20	theme	toreuma	552:558	arg1	teeth					528:532	the teeth	524:532	the teeth of limpet Cellana toreuma	524:558	The study investigates the microstructure, proteomics, and crystallization in the teeth of limpet Cellana toreuma.					
30142254	2	21	theme	unique	396:401	arg1	process					437:443	this unique and fundamental biomineralization process	391:443	this unique and fundamental biomineralization process	391:443	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	10	22	theme	molecular	1381:1389	arg1	levels					1391:1396	the molecular levels	1377:1396	the molecular levels	1377:1396	This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.					
30142254	10	23	theme	room	1474:1477	arg1	temperature					1479:1489	room temperature	1474:1489	room temperature	1474:1489	This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.					
30142254	3	24	from	microstructure	473:486	arg1	teeth					528:532	the teeth	524:532	the teeth of limpet Cellana toreuma	524:558	The study investigates the microstructure, proteomics, and crystallization in the teeth of limpet Cellana toreuma.					
30142254	4	25	theme	tricuspid	611:619	arg1	teeth					621:625	alternatively tricuspid teeth	597:625	alternatively tricuspid teeth	597:625	It is found that the limpets formed alternatively tricuspid teeth and unicuspid teeth.					
30142254	1	26	theme	marine	84:89	arg1	Limpets					72:78	Limpets	72:78	Limpets	72:78	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	26	theme	marine	84:89	arg1	mollusks					91:98	marine mollusks	84:98	marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks	84:210	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	9	27	theme	framework	1191:1199	arg1	chitin					1201:1206	the framework chitin	1187:1206	the framework chitin	1187:1206	Furthermore, the extracted proteins from the cusps of radula or the framework chitin induce packing of crystals and possibly affect crystal polymorphs in vitro.					
30142254	3	28	theme	limpet	537:542	arg1	toreuma					552:558	limpet Cellana toreuma	537:558	limpet Cellana toreuma	537:558	The study investigates the microstructure, proteomics, and crystallization in the teeth of limpet Cellana toreuma.					
30142254	9	29	theme	chitin	1201:1206	arg1	cusps					1168:1172	the cusps	1164:1172	the cusps of radula or the framework chitin	1164:1206	Furthermore, the extracted proteins from the cusps of radula or the framework chitin induce packing of crystals and possibly affect crystal polymorphs in vitro.					
30142254	10	30	theme	hard	1456:1459	arg1	materials					1461:1469	hard materials	1456:1469	hard materials	1456:1469	This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.					
30142254	10	31	from	process	1346:1352	arg1	teeth					1368:1372	the limpet teeth	1357:1372	the limpet teeth	1357:1372	This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.					
30142254	2	32	theme	process	437:443	arg1	understanding					374:386	our understanding	370:386	our understanding of this unique and fundamental biomineralization process	370:443	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	6	33	theme	trailing	828:835	arg1	regions					837:843	the trailing regions	824:843	the trailing regions of the cusps	824:856	In contrast, big nanoneedles resembling chemically synthesized goethite are loosely packed in the trailing regions of the cusps.					
30142254	2	34	dep	ultrastructure	256:269	arg1	the					252:254	the	252:254	the	252:254	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	2	35	theme	biomineralization	419:435	arg1	process					437:443	this unique and fundamental biomineralization process	391:443	this unique and fundamental biomineralization process	391:443	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	1	36	theme	mineralized	109:119	arg1	teeth					121:125	mineralized teeth	109:125	mineralized teeth	109:125	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	36	theme	mineralized	109:119	arg1	strongest					151:159	strongest	151:159	strongest	151:159	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	36	theme	mineralized	109:119	arg1	one					128:130	one	128:130	one	128:130	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	1	36	theme	mineralized	109:119	arg1	hardest					139:145	hardest	139:145	hardest	139:145	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	7	37	theme	whole	887:891	arg1	radula					893:898	the whole radula	883:898	the whole radula	883:898	Proteins extracted from the whole radula, such as ferritin, peroxiredoxin, arginine kinase, GTPase-Rabs, and clathrin, are identified by proteomics.					
30142254	9	38	theme	crystals	1226:1233	arg1	packing					1215:1221	packing	1215:1221	packing of crystals	1215:1233	Furthermore, the extracted proteins from the cusps of radula or the framework chitin induce packing of crystals and possibly affect crystal polymorphs in vitro.					
30142254	7	39	theme	arginine	934:941	arg1	kinase					943:948	arginine kinase	934:948	arginine kinase	934:948	Proteins extracted from the whole radula, such as ferritin, peroxiredoxin, arginine kinase, GTPase-Rabs, and clathrin, are identified by proteomics.					
30142254	1	40	dep	hardest	139:145	arg1	biomaterials					161:172	biomaterials	161:172	biomaterials	161:172	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	8	41	theme	chitin-binding	1089:1102	arg1	CtCBPs					1114:1119	CtCBPs	1114:1119	CtCBPs	1114:1119	A goethite-binding experiment coupled with proteomics and RNA-seq highlights six chitin-binding proteins (CtCBPs).					
30142254	8	41	theme	chitin-binding	1089:1102	arg1	proteins					1104:1111	six chitin-binding proteins	1085:1111	six chitin-binding proteins (CtCBPs)	1085:1120	A goethite-binding experiment coupled with proteomics and RNA-seq highlights six chitin-binding proteins (CtCBPs).					
30142254	9	42	from	cusps	1168:1172	arg1	proteins					1150:1157	the extracted proteins	1136:1157	the extracted proteins from the cusps of radula or the framework chitin	1136:1206	Furthermore, the extracted proteins from the cusps of radula or the framework chitin induce packing of crystals and possibly affect crystal polymorphs in vitro.					
30142254	1	43	theme	intertidal	195:204	arg1	rocks					206:210	intertidal rocks	195:210	intertidal rocks	195:210	Limpets are marine mollusks that use mineralized teeth, one of the hardest and strongest biomaterials, to feed on algae on intertidal rocks.					
30142254	0	44	theme	Teeth	65:69	arg1	Crystallization					35:49	Crystallization	35:49	Crystallization	35:49	The Microstructure, Proteomics and Crystallization of the Limpet Teeth.					
30142254	0	44	theme	Teeth	65:69	arg1	Proteomics					20:29	Proteomics	20:29	Proteomics	20:29	The Microstructure, Proteomics and Crystallization of the Limpet Teeth.					
30142254	0	44	theme	Teeth	65:69	arg1	Microstructure					4:17	Microstructure	4:17	Microstructure	4:17	The Microstructure, Proteomics and Crystallization of the Limpet Teeth.					
30142254	10	45	from	levels	1391:1396	arg1	process					1346:1352	the unique biomineralization process	1317:1352	the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature	1317:1489	This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.					
30142254	10	46	theme	limpet	1361:1366	arg1	teeth					1368:1372	the limpet teeth	1357:1372	the limpet teeth	1357:1372	This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.					
30142254	4	47	theme	unicuspid	631:639	arg1	teeth					641:645	unicuspid teeth	631:645	unicuspid teeth	631:645	It is found that the limpets formed alternatively tricuspid teeth and unicuspid teeth.					
30142254	0	48	theme	Limpet	58:63	arg1	Teeth					65:69	the Limpet Teeth	54:69	the Limpet Teeth	54:69	The Microstructure, Proteomics and Crystallization of the Limpet Teeth.					
30142254	10	49	theme	biomineralization	1328:1344	arg1	process					1346:1352	the unique biomineralization process	1317:1352	the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature	1317:1489	This study provides insight into the unique biomineralization process in the limpet teeth at the molecular levels, which may guide biomimetic strategies aimed at designing hard materials at room temperature.					
30142254	2	50	theme	molecular	319:327	arg1	unknown					352:358	unknown	352:358	unknown	352:358	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	2	50	theme	molecular	319:327	arg1	information					329:339	the molecular information	315:339	the molecular information	315:339	However, most of studies only focus on the ultrastructure and chemical composition of the teeth while the molecular information is largely unknown, limiting our understanding of this unique and fundamental biomineralization process.					
30142254	3	51	from	crystallization	505:519	arg1	teeth					528:532	the teeth	524:532	the teeth of limpet Cellana toreuma	524:558	The study investigates the microstructure, proteomics, and crystallization in the teeth of limpet Cellana toreuma.					
31459263	2	0	theme	DS	437:438	arg1	effect					427:432	the effect	423:432	the effect of DS on the liposome mechanical properties	423:476	Here, we first test the affinity of dextran sulfate (DS) for two different vesicle composition, and afterward, we study the effect of DS on the liposome mechanical properties.					
31459263	4	1	theme	metal	621:625	arg1	ions					627:630	the metal ions	617:630	the metal ions present in the aqueous solution (Na+ and Ca2+)	617:677	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	4	2	theme	present	632:638	arg1	ions					627:630	the metal ions	617:630	the metal ions present in the aqueous solution (Na+ and Ca2+)	617:677	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	7	3	from	kind	952:955	arg1	systems					986:992	drug delivery systems	972:992	drug delivery systems	972:992	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	7	4	theme	biochemical	1081:1091	arg1	factors					1093:1099	other biochemical factors	1075:1099	other biochemical factors	1075:1099	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	7	5	theme	other	1075:1079	arg1	factors					1093:1099	other biochemical factors	1075:1099	other biochemical factors	1075:1099	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	7	6	with	related	1001:1007	arg1	modulation					1016:1025	a modulation	1014:1025	a modulation of the membrane thermodynamic properties	1014:1066	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	1	7	theme	drug	268:271	arg1	probability					249:259	the probability	245:259	the probability of the drug for reaching the target cell	245:300	Lipid vesicles decorated with polysaccharides have been proposed as vehicles for drug delivery because the polymers confer to the vesicles an enhanced stability, increasing the probability of the drug for reaching the target cell.					
31459263	2	8	theme	mechanical	456:465	arg1	properties					467:476	the liposome mechanical properties	443:476	the liposome mechanical properties	443:476	Here, we first test the affinity of dextran sulfate (DS) for two different vesicle composition, and afterward, we study the effect of DS on the liposome mechanical properties.					
31459263	7	9	theme	delivery	977:984	arg1	systems					986:992	drug delivery systems	972:992	drug delivery systems	972:992	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	1	10	theme	Lipid	72:76	arg1	vesicles					78:85	Lipid vesicles	72:85	Lipid vesicles decorated with polysaccharides	72:116	Lipid vesicles decorated with polysaccharides have been proposed as vehicles for drug delivery because the polymers confer to the vesicles an enhanced stability, increasing the probability of the drug for reaching the target cell.					
31459263	1	10	theme	Lipid	72:76	arg1	vehicles					140:147	vehicles	140:147	vehicles for drug delivery	140:165	Lipid vesicles decorated with polysaccharides have been proposed as vehicles for drug delivery because the polymers confer to the vesicles an enhanced stability, increasing the probability of the drug for reaching the target cell.					
31459263	2	11	theme	liposome	447:454	arg1	properties					467:476	the liposome mechanical properties	443:476	the liposome mechanical properties	443:476	Here, we first test the affinity of dextran sulfate (DS) for two different vesicle composition, and afterward, we study the effect of DS on the liposome mechanical properties.					
31459263	2	12	from	effect	427:432	arg1	properties					467:476	the liposome mechanical properties	443:476	the liposome mechanical properties	443:476	Here, we first test the affinity of dextran sulfate (DS) for two different vesicle composition, and afterward, we study the effect of DS on the liposome mechanical properties.					
31459263	5	13	theme	packed	770:775	arg1	lipids					777:782	closely packed lipids	762:782	closely packed lipids	762:782	Binding occurs preferentially in regions of closely packed lipids.					
31459263	7	14	theme	properties	1057:1066	arg1	modulation					1016:1025	a modulation	1014:1025	a modulation of the membrane thermodynamic properties	1014:1066	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	4	15	attach	present	632:638	arg1	solution					655:662	the aqueous solution	643:662	the aqueous solution (Na+ and Ca2+)	643:677	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	4	15	attach	present	632:638	arg2	ions					627:630	the metal ions	617:630	the metal ions present in the aqueous solution (Na+ and Ca2+)	617:677	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	4	16	dep	solution	655:662	arg1	Ca2+					673:676	Ca2+	673:676	Ca2+	673:676	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	4	16	dep	solution	655:662	arg1	Na+					665:667	Na+	665:667	Na+	665:667	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	5	17	theme	lipids	777:782	arg1	regions					751:757	regions	751:757	regions of closely packed lipids	751:782	Binding occurs preferentially in regions of closely packed lipids.					
31459263	0	18	theme	Mechanical	0:9	arg1	Stability					11:19	Mechanical Stability	0:19	Mechanical Stability of Lipid Membranes	0:38	Mechanical Stability of Lipid Membranes Decorated with Dextran Sulfate.					
31459263	6	19	theme	none	876:879	arg1	rigidity					864:871	the membrane rigidity	851:871	the membrane rigidity of none of the vesicles	851:895	Strikingly, DS did not affect the stability against detergent and the membrane rigidity of none of the vesicles.					
31459263	6	19	theme	none	876:879	arg1	stability					819:827	the stability	815:827	the stability against detergent	815:845	Strikingly, DS did not affect the stability against detergent and the membrane rigidity of none of the vesicles.					
31459263	4	20	theme	aqueous	647:653	arg1	solution					655:662	the aqueous solution	643:662	the aqueous solution (Na+ and Ca2+)	643:677	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	3	21	theme	tested	510:515	arg1	compositions					526:537	both tested membrane compositions	505:537	both tested membrane compositions	505:537	We found that DS binds to both tested membrane compositions.					
31459263	0	22	theme	Lipid	24:28	arg1	Membranes					30:38	Lipid Membranes	24:38	Lipid Membranes	24:38	Mechanical Stability of Lipid Membranes Decorated with Dextran Sulfate.					
31459263	4	23	theme	anionic	571:577	arg1	membranes					579:587	the anionic membranes	567:587	the anionic membranes studied here	567:600	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	7	24	theme	thermodynamic	1043:1055	arg1	properties					1057:1066	the membrane thermodynamic properties	1030:1066	the membrane thermodynamic properties	1030:1066	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	7	25	theme	polymers	960:967	arg1	kind					952:955	this kind	947:955	this kind of polymers in drug delivery systems	947:992	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	2	26	theme	vesicle	378:384	arg1	composition					386:396	two different vesicle composition	364:396	two different vesicle composition	364:396	Here, we first test the affinity of dextran sulfate (DS) for two different vesicle composition, and afterward, we study the effect of DS on the liposome mechanical properties.					
31459263	1	27	theme	target	290:295	arg1	cell					297:300	the target cell	286:300	the target cell	286:300	Lipid vesicles decorated with polysaccharides have been proposed as vehicles for drug delivery because the polymers confer to the vesicles an enhanced stability, increasing the probability of the drug for reaching the target cell.					
31459263	6	28	theme	membrane	855:862	arg1	rigidity					864:871	the membrane rigidity	851:871	the membrane rigidity of none of the vesicles	851:895	Strikingly, DS did not affect the stability against detergent and the membrane rigidity of none of the vesicles.					
31459263	2	29	theme	different	368:376	arg1	composition					386:396	two different vesicle composition	364:396	two different vesicle composition	364:396	Here, we first test the affinity of dextran sulfate (DS) for two different vesicle composition, and afterward, we study the effect of DS on the liposome mechanical properties.					
31459263	0	30	theme	Membranes	30:38	arg1	Stability					11:19	Mechanical Stability	0:19	Mechanical Stability of Lipid Membranes	0:38	Mechanical Stability of Lipid Membranes Decorated with Dextran Sulfate.					
31459263	4	31	theme	Ca2+	712:715	arg1	presence					700:707	the presence	696:707	the presence of Ca2+	696:715	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	1	32	theme	enhanced	214:221	arg1	stability					223:231	an enhanced stability	211:231	an enhanced stability	211:231	Lipid vesicles decorated with polysaccharides have been proposed as vehicles for drug delivery because the polymers confer to the vesicles an enhanced stability, increasing the probability of the drug for reaching the target cell.					
31459263	0	33	theme	Dextran	55:61	arg1	Sulfate					63:69	Dextran Sulfate	55:69	Dextran Sulfate	55:69	Mechanical Stability of Lipid Membranes Decorated with Dextran Sulfate.					
31459263	3	34	theme	membrane	517:524	arg1	compositions					526:537	both tested membrane compositions	505:537	both tested membrane compositions	505:537	We found that DS binds to both tested membrane compositions.					
31459263	7	35	theme	proposed	908:915	arg1	related					1001:1007	related	1001:1007	related	1001:1007	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	7	35	theme	proposed	908:915	arg1	increase					927:934	the proposed stability increase	904:934	the proposed stability increase induced by this kind of polymers in drug delivery systems	904:992	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	4	36	with	interaction	544:554	arg1	membranes					579:587	the anionic membranes	567:587	the anionic membranes studied here	567:600	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	4	37	from	solution	655:662	arg1	present					632:638	present	632:638	present	632:638	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	7	38	theme	membrane	1034:1041	arg1	properties					1057:1066	the membrane thermodynamic properties	1030:1066	the membrane thermodynamic properties	1030:1066	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	7	39	theme	stability	917:925	arg1	related					1001:1007	related	1001:1007	related	1001:1007	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	7	39	theme	stability	917:925	arg1	increase					927:934	the proposed stability increase	904:934	the proposed stability increase induced by this kind of polymers in drug delivery systems	904:992	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	1	40	theme	drug	153:156	arg1	delivery					158:165	drug delivery	153:165	drug delivery	153:165	Lipid vesicles decorated with polysaccharides have been proposed as vehicles for drug delivery because the polymers confer to the vesicles an enhanced stability, increasing the probability of the drug for reaching the target cell.					
31459263	7	41	theme	drug	972:975	arg1	systems					986:992	drug delivery systems	972:992	drug delivery systems	972:992	Thus, the proposed stability increase induced by this kind of polymers in drug delivery systems is not related with a modulation of the membrane thermodynamic properties but to other biochemical factors.					
31459263	2	42	theme	sulfate	347:353	arg1	affinity					327:334	the affinity	323:334	the affinity of dextran sulfate (DS) for two different vesicle composition	323:396	Here, we first test the affinity of dextran sulfate (DS) for two different vesicle composition, and afterward, we study the effect of DS on the liposome mechanical properties.					
31459263	2	43	theme	dextran	339:345	arg1	DS					356:357	DS	356:357	DS	356:357	Here, we first test the affinity of dextran sulfate (DS) for two different vesicle composition, and afterward, we study the effect of DS on the liposome mechanical properties.					
31459263	2	43	theme	dextran	339:345	arg1	sulfate					347:353	dextran sulfate	339:353	dextran sulfate (DS)	339:358	Here, we first test the affinity of dextran sulfate (DS) for two different vesicle composition, and afterward, we study the effect of DS on the liposome mechanical properties.					
31459263	4	44	from	present	632:638	arg1	solution					655:662	the aqueous solution	643:662	the aqueous solution (Na+ and Ca2+)	643:677	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
31459263	4	45	theme	DS	559:560	arg1	interaction					544:554	The interaction	540:554	The interaction of DS with the anionic membranes studied here	540:600	The interaction of DS with the anionic membranes studied here is mediated by the metal ions present in the aqueous solution (Na+ and Ca2+), being higher in the presence of Ca2+.					
30676876	8	0	theme	beans	1725:1729	arg1	potential					1683:1691	anti-inflammatory potential	1665:1691	anti-inflammatory potential	1665:1691	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	8	0	theme	beans	1725:1729	arg1	potential					1709:1717	antioxidant potential	1697:1717	antioxidant potential	1697:1717	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	1	1	theme	anti-inflammatory	378:394	arg1	potential					412:420	in vitro anti-inflammatory and antioxidant potential	369:420	in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM)	369:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	2	theme	proximate	342:350	arg1	composition					352:362	proximate composition	342:362	proximate composition	342:362	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	9	3	dep	in	1916:1917	arg1	vitro					1919:1923	vitro	1919:1923	vitro	1919:1923	Conclusion: The differential process showed considerable changes in the proximate composition, in vitro digestibility, and biological potential.					
30676876	1	4	theme	processes	234:242	arg1	effect					206:211	the effect	202:211	the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM)	202:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	2	5	dep	L-DOPA	481:486	arg1	The					477:479	The	477:479	The	477:479	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	2	5	dep	L-DOPA	481:486	arg1	quantification					514:527	quantification	514:527	quantification	514:527	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	2	6	theme	samples	542:548	arg1	acids					508:512	major phenolics acids	492:512	major phenolics acids	492:512	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	2	6	theme	samples	542:548	arg1	L-DOPA					481:486	L-DOPA	481:486	L-DOPA	481:486	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	1	7	with	protein	290:296	arg1	potential					412:420	in vitro anti-inflammatory and antioxidant potential	369:420	in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM)	369:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	3	8	theme	in	692:693	arg1	protein					701:707	in vitro protein and starch digestibility	692:732	protein	701:707	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	10	9	theme	maximum	2057:2063	arg1	potential					2077:2085	maximum nutritional potential	2057:2085	maximum nutritional potential with health benefits	2057:2106	The present study recommends the utilization of MM beans after autoclaving and boiling for maximum nutritional potential with health benefits.					
30676876	1	10	theme	antioxidant	400:410	arg1	potential					412:420	in vitro anti-inflammatory and antioxidant potential	369:420	in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM)	369:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	11	theme	L-DOPA	247:252	arg1	level					254:258	L-DOPA level	247:258	L-DOPA level	247:258	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	2	12	theme	high-performance	579:594	arg1	RP-HPLC					619:625	RP-HPLC	619:625	RP-HPLC	619:625	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	2	12	theme	high-performance	579:594	arg1	chromatography					603:616	reverse-phase high-performance liquid chromatography	565:616	a reverse-phase high-performance liquid chromatography (RP-HPLC) technique	563:636	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	4	13	theme	samples	840:846	arg1	activities					826:835	The anti-inflammatory activities	804:835	The anti-inflammatory activities of samples	804:846	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	1	14	with	contents	271:278	arg1	potential					412:420	in vitro anti-inflammatory and antioxidant potential	369:420	in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM)	369:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	8	15	theme	antioxidant	1798:1808	arg1	molecules					1810:1818	antioxidant molecules	1798:1818	antioxidant molecules	1798:1818	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	3	16	theme	starch	713:718	arg1	digestibility					720:732	in vitro protein and starch digestibility	692:732	digestibility	720:732	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	6	17	theme	processed	1221:1229	arg1	beans					1234:1238	The processed MM beans	1217:1238	The processed MM beans	1217:1238	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	1	18	theme	macrocarpa	432:441	arg1	potential					412:420	in vitro anti-inflammatory and antioxidant potential	369:420	in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM)	369:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	2	19	theme	major	492:496	arg1	acids					508:512	major phenolics acids	492:512	major phenolics acids	492:512	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	8	20	dep	potential	1683:1691	arg1	the					1661:1663	the	1661:1663	the	1661:1663	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	6	21	theme	phytic	1290:1295	arg1	%					1308:1308	25.78%	1303:1308	25.78%	1303:1308	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	21	theme	phytic	1290:1295	arg1	acid					1297:1300	phytic acid	1290:1300	phytic acid (25.78%)	1290:1309	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	1	22	theme	present	179:185	arg1	investigation					187:199	the present investigation	175:199	the present investigation	175:199	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	7	23	theme	seed	1519:1522	arg1	sample					1524:1529	the raw seed sample	1511:1529	the raw seed sample	1511:1529	RP-HPLC quantification of major phenolics acids was also affected by the differential process as compare to the raw seed sample.					
30676876	4	24	theme	membrane	898:905	arg1	test					921:924	human red blood cells (HRBCs) membrane stabilization test	868:924	human red blood cells (HRBCs) membrane stabilization test	868:924	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	0	25	from	Digestibility	57:69	arg1	L-DOPA					21:26	L-DOPA	21:26	L-DOPA	21:26	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	10	26	theme	beans	2017:2021	arg1	utilization					1999:2009	the utilization	1995:2009	the utilization of MM beans	1995:2021	The present study recommends the utilization of MM beans after autoclaving and boiling for maximum nutritional potential with health benefits.					
30676876	3	27	theme	samples	741:747	arg1	composition					649:659	Proximate composition	639:659	Proximate composition	639:659	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	3	27	theme	samples	741:747	arg1	digestibility					720:732	in vitro protein and starch digestibility	692:732	digestibility	720:732	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	3	27	theme	samples	741:747	arg1	quantification					672:685	elemental quantification	662:685	elemental quantification	662:685	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	3	27	theme	samples	741:747	arg1	protein					701:707	in vitro protein and starch digestibility	692:732	protein	701:707	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	6	28	theme	L-DOPA	1274:1279	arg1	autoclaving					1366:1376	autoclaving	1366:1376	autoclaving	1366:1376	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	28	theme	L-DOPA	1274:1279	arg1	reduction					1261:1269	a significant reduction	1247:1269	a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling	1247:1363	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	28	theme	L-DOPA	1274:1279	arg1	processes					1392:1400	roasting processes	1383:1400	roasting processes	1383:1400	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	4	29	theme	red	874:876	arg1	HRBCs					891:895	HRBCs	891:895	HRBCs	891:895	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	4	29	theme	red	874:876	arg1	cells					884:888	human red blood cells	868:888	human red blood cells (HRBCs) membrane stabilization test	868:924	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	8	30	dep	improvement	1577:1587	arg1	digestibility					1638:1650	digestibility	1638:1650	digestibility	1638:1650	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	0	31	theme	Legume	134:139	arg1	Digestibility					57:69	In Vitro Protein and Starch Digestibility	29:69	Digestibility	57:69	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	31	theme	Legume	134:139	arg1	Composition					82:92	Proximate Composition	72:92	Proximate Composition	72:92	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	31	theme	Legume	134:139	arg1	Activities					110:119	Biological Activities	99:119	Biological Activities of Promising Legume	99:139	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	31	theme	Legume	134:139	arg1	Effect					11:16	Processing Effect	0:16	Processing Effect on L-DOPA	0:26	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	31	theme	Legume	134:139	arg1	Protein					38:44	In Vitro Protein and Starch Digestibility	29:69	Protein	38:44	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	5	32	theme	beans	1017:1021	arg1	potential					994:1002	Antioxidant potential	982:1002	Antioxidant potential of processed beans	982:1021	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	4	33	theme	cells	884:888	arg1	test					921:924	human red blood cells (HRBCs) membrane stabilization test	868:924	human red blood cells (HRBCs) membrane stabilization test	868:924	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	8	34	dep	showed	1557:1562	arg1	whereas					1653:1659	whereas	1653:1659	whereas	1653:1659	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	1	35	from	effect	206:211	arg1	level					254:258	L-DOPA level	247:258	L-DOPA level	247:258	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	35	from	effect	206:211	arg1	contents					271:278	phenolics contents	261:278	phenolics contents	261:278	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	35	from	effect	206:211	arg1	IVPD					299:302	IVPD	299:302	IVPD	299:302	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	35	from	effect	206:211	arg1	composition					352:362	proximate composition	342:362	proximate composition	342:362	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	35	from	effect	206:211	arg1	protein					290:296	in vitro protein	281:296	in vitro protein (IVPD)	281:303	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	4	36	theme	albumin	943:949	arg1	assay					975:979	a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay	866:979	a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay	866:979	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	9	37	theme	considerable	1865:1876	arg1	changes					1878:1884	considerable changes	1865:1884	considerable changes in the proximate composition, in vitro digestibility, and biological potential	1865:1963	Conclusion: The differential process showed considerable changes in the proximate composition, in vitro digestibility, and biological potential.					
30676876	5	38	theme	reducing/antioxidant	1167:1186	arg1	FRAP					1195:1198	FRAP	1195:1198	FRAP	1195:1198	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	5	38	theme	reducing/antioxidant	1167:1186	arg1	power					1188:1192	ferric reducing/antioxidant power	1160:1192	ferric reducing/antioxidant power (FRAP) assay	1160:1205	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	2	39	theme	phenolics	498:506	arg1	acids					508:512	major phenolics acids	492:512	major phenolics acids	492:512	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	8	40	theme	anti-inflammatory	1665:1681	arg1	potential					1683:1691	anti-inflammatory potential	1665:1691	anti-inflammatory potential	1665:1691	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	4	41	theme	test	921:924	arg1	assay					975:979	a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay	866:979	a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay	866:979	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	9	42	theme	proximate	1893:1901	arg1	composition					1903:1913	the proximate composition	1889:1913	the proximate composition	1889:1913	Conclusion: The differential process showed considerable changes in the proximate composition, in vitro digestibility, and biological potential.					
30676876	3	43	theme	spectrophotometric	775:792	arg1	analysis					794:801	spectrophotometric analysis	775:801	spectrophotometric analysis	775:801	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	0	44	theme	Proximate	72:80	arg1	Composition					82:92	Proximate Composition	72:92	Proximate Composition	72:92	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	1	45	theme	starch	309:314	arg1	digestibility					316:328	starch digestibility	309:328	starch digestibility (IVSD)	309:335	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	45	theme	starch	309:314	arg1	IVSD					331:334	IVSD	331:334	IVSD	331:334	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	4	46	theme	bovine	930:935	arg1	albumin					943:949	bovine serum albumin	930:949	bovine serum albumin (BSA)	930:955	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	4	46	theme	bovine	930:935	arg1	BSA					952:954	BSA	952:954	BSA	952:954	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	2	47	theme	liquid	596:601	arg1	RP-HPLC					619:625	RP-HPLC	619:625	RP-HPLC	619:625	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	2	47	theme	liquid	596:601	arg1	chromatography					603:616	reverse-phase high-performance liquid chromatography	565:616	a reverse-phase high-performance liquid chromatography (RP-HPLC) technique	563:636	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	5	48	theme	scavenging	1087:1096	arg1	activity					1098:1105	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1042:1105	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1042:1105	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	4	49	theme	anti-inflammatory	808:824	arg1	activities					826:835	The anti-inflammatory activities	804:835	The anti-inflammatory activities of samples	804:846	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	1	50	with	composition	352:362	arg1	potential					412:420	in vitro anti-inflammatory and antioxidant potential	369:420	in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM)	369:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	51	dep	in	281:282	arg1	vitro					284:288	vitro	284:288	vitro	284:288	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	0	52	theme	Processing	0:9	arg1	Effect					11:16	Processing Effect	0:16	Processing Effect on L-DOPA	0:26	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	8	53	theme	MM	1722:1723	arg1	beans					1725:1729	MM beans	1722:1729	MM beans	1722:1729	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	8	54	dep	in	1592:1593	arg1	vitro					1595:1599	vitro	1595:1599	vitro	1595:1599	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	6	55	from	autoclaving	1366:1376	arg1	boiling					1357:1363	the boiling	1353:1363	the boiling	1353:1363	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	56	theme	saponin	1333:1339	arg1	autoclaving					1366:1376	autoclaving	1366:1376	autoclaving	1366:1376	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	56	theme	saponin	1333:1339	arg1	reduction					1261:1269	a significant reduction	1247:1269	a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling	1247:1363	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	56	theme	saponin	1333:1339	arg1	processes					1392:1400	roasting processes	1383:1400	roasting processes	1383:1400	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	1	57	theme	phenolics	261:269	arg1	contents					271:278	phenolics contents	261:278	phenolics contents	261:278	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	7	58	theme	major	1429:1433	arg1	acids					1445:1449	major phenolics acids	1429:1449	major phenolics acids	1429:1449	RP-HPLC quantification of major phenolics acids was also affected by the differential process as compare to the raw seed sample.					
30676876	4	59	theme	anti-denaturation	957:973	arg1	assay					975:979	a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay	866:979	a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay	866:979	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	9	60	theme	biological	1944:1953	arg1	potential					1955:1963	biological potential	1944:1963	biological potential	1944:1963	Conclusion: The differential process showed considerable changes in the proximate composition, in vitro digestibility, and biological potential.					
30676876	10	61	with	potential	2077:2085	arg1	benefits					2099:2106	health benefits	2092:2106	health benefits	2092:2106	The present study recommends the utilization of MM beans after autoclaving and boiling for maximum nutritional potential with health benefits.					
30676876	3	62	dep	in	692:693	arg1	vitro					695:699	vitro	695:699	vitro	695:699	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	5	63	theme	processed	1007:1015	arg1	beans					1017:1021	processed beans	1007:1021	processed beans	1007:1021	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	3	64	theme	Proximate	639:647	arg1	composition					649:659	Proximate composition	639:659	Proximate composition	639:659	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	1	65	theme	cooking	226:232	arg1	processes					234:242	different cooking processes	216:242	different cooking processes	216:242	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	10	66	theme	present	1970:1976	arg1	study					1978:1982	The present study	1966:1982	The present study	1966:1982	The present study recommends the utilization of MM beans after autoclaving and boiling for maximum nutritional potential with health benefits.					
30676876	6	67	theme	significant	1249:1259	arg1	reduction					1261:1269	a significant reduction	1247:1269	a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling	1247:1363	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	2	68	theme	processed	532:540	arg1	samples					542:548	processed samples	532:548	processed samples	532:548	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	10	69	theme	MM	2014:2015	arg1	beans					2017:2021	MM beans	2014:2021	MM beans	2014:2021	The present study recommends the utilization of MM beans after autoclaving and boiling for maximum nutritional potential with health benefits.					
30676876	8	70	theme	processed	1753:1761	arg1	samples					1763:1769	the processed samples	1749:1769	the processed samples	1749:1769	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	8	71	theme	in	1592:1593	arg1	protein					1601:1607	in vitro protein	1592:1607	in vitro protein	1592:1607	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	2	72	theme	reverse-phase	565:577	arg1	RP-HPLC					619:625	RP-HPLC	619:625	RP-HPLC	619:625	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	2	72	theme	reverse-phase	565:577	arg1	chromatography					603:616	reverse-phase high-performance liquid chromatography	565:616	a reverse-phase high-performance liquid chromatography (RP-HPLC) technique	563:636	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	6	73	theme	MM	1231:1232	arg1	beans					1234:1238	The processed MM beans	1217:1238	The processed MM beans	1217:1238	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	1	74	theme	Mucuna	425:430	arg1	MM					444:445	MM	444:445	MM	444:445	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	74	theme	Mucuna	425:430	arg1	macrocarpa					432:441	Mucuna macrocarpa	425:441	Mucuna macrocarpa (MM)	425:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	8	75	theme	protein	1601:1607	arg1	%					1615:1615	26.93%	1610:1615	26.93%	1610:1615	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	8	75	theme	protein	1601:1607	arg1	improvement					1577:1587	considerable improvement	1564:1587	considerable improvement of in vitro protein (26.93%)	1564:1616	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	1	76	with	level	254:258	arg1	potential					412:420	in vitro anti-inflammatory and antioxidant potential	369:420	in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM)	369:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	6	77	theme	acid	1297:1300	arg1	autoclaving					1366:1376	autoclaving	1366:1376	autoclaving	1366:1376	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	77	theme	acid	1297:1300	arg1	reduction					1261:1269	a significant reduction	1247:1269	a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling	1247:1363	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	77	theme	acid	1297:1300	arg1	processes					1392:1400	roasting processes	1383:1400	roasting processes	1383:1400	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	7	78	theme	raw	1515:1517	arg1	sample					1524:1529	the raw seed sample	1511:1529	the raw seed sample	1511:1529	RP-HPLC quantification of major phenolics acids was also affected by the differential process as compare to the raw seed sample.					
30676876	0	79	theme	Promising	124:132	arg1	Legume					134:139	Promising Legume	124:139	Promising Legume	124:139	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	5	80	theme	1,1-diphenyl-2-picrylhydrazyl	1042:1070	arg1	activity					1098:1105	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1042:1105	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1042:1105	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	6	81	theme	roasting	1383:1390	arg1	processes					1392:1400	roasting processes	1383:1400	roasting processes	1383:1400	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	10	82	theme	nutritional	2065:2075	arg1	potential					2077:2085	maximum nutritional potential	2057:2085	maximum nutritional potential with health benefits	2057:2106	The present study recommends the utilization of MM beans after autoclaving and boiling for maximum nutritional potential with health benefits.					
30676876	8	83	theme	molecules	1810:1818	arg1	reduction					1785:1793	a reduction	1783:1793	a reduction of antioxidant molecules	1783:1818	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	5	84	theme	DPPH	1073:1076	arg1	activity					1098:1105	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1042:1105	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1042:1105	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	0	85	dep	macrocarpa	149:158	arg1	Composition					82:92	Proximate Composition	72:92	Proximate Composition	72:92	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	85	dep	macrocarpa	149:158	arg1	Protein					38:44	In Vitro Protein and Starch Digestibility	29:69	Protein	38:44	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	85	dep	macrocarpa	149:158	arg1	Effect					11:16	Processing Effect	0:16	Processing Effect on L-DOPA	0:26	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	85	dep	macrocarpa	149:158	arg1	Activities					110:119	Biological Activities	99:119	Biological Activities of Promising Legume	99:139	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	4	86	theme	human	868:872	arg1	HRBCs					891:895	HRBCs	891:895	HRBCs	891:895	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	4	86	theme	human	868:872	arg1	cells					884:888	human red blood cells	868:888	human red blood cells (HRBCs) membrane stabilization test	868:924	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	9	87	theme	differential	1837:1848	arg1	process					1850:1856	The differential process	1833:1856	The differential process	1833:1856	Conclusion: The differential process showed considerable changes in the proximate composition, in vitro digestibility, and biological potential.					
30676876	7	88	theme	differential	1476:1487	arg1	process					1489:1495	the differential process	1472:1495	the differential process as compare to the raw seed sample	1472:1529	RP-HPLC quantification of major phenolics acids was also affected by the differential process as compare to the raw seed sample.					
30676876	4	89	theme	blood	878:882	arg1	HRBCs					891:895	HRBCs	891:895	HRBCs	891:895	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	4	89	theme	blood	878:882	arg1	cells					884:888	human red blood cells	868:888	human red blood cells (HRBCs) membrane stabilization test	868:924	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	0	90	from	Composition	82:92	arg1	L-DOPA					21:26	L-DOPA	21:26	L-DOPA	21:26	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	91	theme	Starch	50:55	arg1	Digestibility					57:69	In Vitro Protein and Starch Digestibility	29:69	Digestibility	57:69	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	1	92	with	digestibility	316:328	arg1	potential					412:420	in vitro anti-inflammatory and antioxidant potential	369:420	in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM)	369:446	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	4	93	theme	serum	937:941	arg1	albumin					943:949	bovine serum albumin	930:949	bovine serum albumin (BSA)	930:955	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	4	93	theme	serum	937:941	arg1	BSA					952:954	BSA	952:954	BSA	952:954	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	6	94	theme	tannin	1312:1317	arg1	autoclaving					1366:1376	autoclaving	1366:1376	autoclaving	1366:1376	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	94	theme	tannin	1312:1317	arg1	reduction					1261:1269	a significant reduction	1247:1269	a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling	1247:1363	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	6	94	theme	tannin	1312:1317	arg1	processes					1392:1400	roasting processes	1383:1400	roasting processes	1383:1400	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	5	95	theme	N	1111:1111	arg1	assays					1149:1154	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays	1042:1154	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays	1042:1154	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	0	96	theme	Biological	99:108	arg1	Activities					110:119	Biological Activities	99:119	Biological Activities of Promising Legume	99:139	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	97	from	Effect	11:16	arg1	L-DOPA					21:26	L-DOPA	21:26	L-DOPA	21:26	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	0	98	theme	Mucuna	142:147	arg1	macrocarpa					149:158	Mucuna macrocarpa	142:158	Mucuna macrocarpa	142:158	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	7	99	theme	acids	1445:1449	arg1	quantification					1411:1424	RP-HPLC quantification	1403:1424	RP-HPLC quantification of major phenolics acids	1403:1449	RP-HPLC quantification of major phenolics acids was also affected by the differential process as compare to the raw seed sample.					
30676876	4	100	theme	stabilization	907:919	arg1	test					921:924	human red blood cells (HRBCs) membrane stabilization test	868:924	human red blood cells (HRBCs) membrane stabilization test	868:924	The anti-inflammatory activities of samples were evaluated by a human red blood cells (HRBCs) membrane stabilization test and bovine serum albumin (BSA) anti-denaturation assay.					
30676876	0	101	from	Protein	38:44	arg1	L-DOPA					21:26	L-DOPA	21:26	L-DOPA	21:26	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	2	102	theme	chromatography	603:616	arg1	technique					628:636	a reverse-phase high-performance liquid chromatography (RP-HPLC) technique	563:636	a reverse-phase high-performance liquid chromatography (RP-HPLC) technique	563:636	Methods: The L-DOPA and major phenolics acids quantification of processed samples were done by a reverse-phase high-performance liquid chromatography (RP-HPLC) technique.					
30676876	5	103	theme	radical	1079:1085	arg1	activity					1098:1105	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1042:1105	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity	1042:1105	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	10	104	theme	health	2092:2097	arg1	benefits					2099:2106	health benefits	2092:2106	health benefits	2092:2106	The present study recommends the utilization of MM beans after autoclaving and boiling for maximum nutritional potential with health benefits.					
30676876	9	105	from	changes	1878:1884	arg1	potential					1955:1963	biological potential	1944:1963	biological potential	1944:1963	Conclusion: The differential process showed considerable changes in the proximate composition, in vitro digestibility, and biological potential.					
30676876	9	105	from	changes	1878:1884	arg1	composition					1903:1913	the proximate composition	1889:1913	the proximate composition	1889:1913	Conclusion: The differential process showed considerable changes in the proximate composition, in vitro digestibility, and biological potential.					
30676876	9	105	from	changes	1878:1884	arg1	digestibility					1925:1937	in vitro digestibility	1916:1937	in vitro digestibility	1916:1937	Conclusion: The differential process showed considerable changes in the proximate composition, in vitro digestibility, and biological potential.					
30676876	9	106	theme	in	1916:1917	arg1	digestibility					1925:1937	in vitro digestibility	1916:1937	in vitro digestibility	1916:1937	Conclusion: The differential process showed considerable changes in the proximate composition, in vitro digestibility, and biological potential.					
30676876	5	107	theme	activity	1098:1105	arg1	assays					1149:1154	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays	1042:1154	1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays	1042:1154	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	8	108	theme	antioxidant	1697:1707	arg1	potential					1709:1717	antioxidant potential	1697:1717	antioxidant potential	1697:1717	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	6	109	from	processes	1392:1400	arg1	boiling					1357:1363	the boiling	1353:1363	the boiling	1353:1363	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
30676876	5	110	theme	ferric	1160:1165	arg1	FRAP					1195:1198	FRAP	1195:1198	FRAP	1195:1198	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	5	110	theme	ferric	1160:1165	arg1	power					1188:1192	ferric reducing/antioxidant power	1160:1192	ferric reducing/antioxidant power (FRAP) assay	1160:1205	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	0	111	theme	In	29:30	arg1	Protein					38:44	In Vitro Protein and Starch Digestibility	29:69	Protein	38:44	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	5	112	theme	power	1188:1192	arg1	assay					1201:1205	ferric reducing/antioxidant power (FRAP) assay	1160:1205	ferric reducing/antioxidant power (FRAP) assay	1160:1205	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	7	113	theme	phenolics	1435:1443	arg1	acids					1445:1449	major phenolics acids	1429:1449	major phenolics acids	1429:1449	RP-HPLC quantification of major phenolics acids was also affected by the differential process as compare to the raw seed sample.					
30676876	5	114	theme	Antioxidant	982:992	arg1	potential					994:1002	Antioxidant potential	982:1002	Antioxidant potential of processed beans	982:1021	Antioxidant potential of processed beans was carried out by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and N,N-dimethyl-p-phenylendiamine (DMPD) assays and ferric reducing/antioxidant power (FRAP) assay.					
30676876	1	115	theme	in	281:282	arg1	IVPD					299:302	IVPD	299:302	IVPD	299:302	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	1	115	theme	in	281:282	arg1	protein					290:296	in vitro protein	281:296	in vitro protein (IVPD)	281:303	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	0	116	dep	In	29:30	arg1	Vitro					32:36	Vitro	32:36	Vitro	32:36	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	8	117	theme	processed	1536:1544	arg1	seeds					1546:1550	The processed seeds	1532:1550	The processed seeds	1532:1550	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	7	118	theme	RP-HPLC	1403:1409	arg1	quantification					1411:1424	RP-HPLC quantification	1403:1424	RP-HPLC quantification of major phenolics acids	1403:1449	RP-HPLC quantification of major phenolics acids was also affected by the differential process as compare to the raw seed sample.					
30676876	0	119	from	Activities	110:119	arg1	L-DOPA					21:26	L-DOPA	21:26	L-DOPA	21:26	Processing Effect on L-DOPA, In Vitro Protein and Starch Digestibility, Proximate Composition, and Biological Activities of Promising Legume: Mucuna macrocarpa.					
30676876	3	120	theme	elemental	662:670	arg1	quantification					672:685	elemental quantification	662:685	elemental quantification	662:685	Proximate composition, elemental quantification, and in vitro protein and starch digestibility of the samples were carried out by using spectrophotometric analysis.					
30676876	1	121	theme	different	216:224	arg1	processes					234:242	different cooking processes	216:242	different cooking processes	216:242	Objective: In the present investigation, the effect of different cooking processes on L-DOPA level, phenolics contents, in vitro protein (IVPD) and starch digestibility (IVSD), and proximate composition with in vitro anti-inflammatory and antioxidant potential of Mucuna macrocarpa (MM) has been evaluated.					
30676876	8	122	theme	considerable	1564:1575	arg1	%					1615:1615	26.93%	1610:1615	26.93%	1610:1615	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	8	122	theme	considerable	1564:1575	arg1	improvement					1577:1587	considerable improvement	1564:1587	considerable improvement of in vitro protein (26.93%)	1564:1616	The processed seeds also showed considerable improvement of in vitro protein (26.93%) and starch (20.30%) digestibility, whereas the anti-inflammatory potential and antioxidant potential of MM beans were decreased in the processed samples, indicating a reduction of antioxidant molecules.					
30676876	6	123	from	reduction	1261:1269	arg1	boiling					1357:1363	the boiling	1353:1363	the boiling	1353:1363	Results: The processed MM beans showed a significant reduction of L-DOPA (6.30%), phytic acid (25.78%), tannin (19.79%), and saponin (25.59%) in the boiling, autoclaving, and roasting processes.					
29182761	4	0	theme	Generalized	567:577	arg1	models					586:591	Generalized linear models	567:591	Generalized linear models	567:591	Generalized linear models, Fisher's exact test, and Pearson's correlation coefficient assessed sugar, aldehyde, and nicotine concentration associations.					
29182761	7	1	theme	acetaldehyde	979:990	arg1	levels					1006:1011	Formaldehyde, acetaldehyde, and acrolein levels	965:1011	Formaldehyde, acetaldehyde, and acrolein levels	965:1011	Formaldehyde, acetaldehyde, and acrolein levels exceeded the limits of quantification in 72%, 84%, and 75% of the samples.					
29182761	6	2	theme	Sucrose	839:845	arg1	levels					847:852	Sucrose levels	839:852	Sucrose levels	839:852	Sucrose levels were significantly higher than glucose [χ2(1) = 85.9, p < .0001] and fructose [χ2(1) = 10.6, p = .001] levels.					
29182761	4	3	theme	correlation	629:639	arg1	coefficient					641:651	Pearson's correlation coefficient	619:651	Pearson's correlation coefficient	619:651	Generalized linear models, Fisher's exact test, and Pearson's correlation coefficient assessed sugar, aldehyde, and nicotine concentration associations.					
29182761	12	4	dep	Implications	1865:1876	arg1	inform					1891:1896	inform	1891:1896	can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking	1887:2177	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	7	5	theme	Formaldehyde	965:976	arg1	levels					1006:1011	Formaldehyde, acetaldehyde, and acrolein levels	965:1011	Formaldehyde, acetaldehyde, and acrolein levels	965:1011	Formaldehyde, acetaldehyde, and acrolein levels exceeded the limits of quantification in 72%, 84%, and 75% of the samples.					
29182761	3	6	theme	chromatography	509:522	arg1	spectrometry					529:540	liquid chromatography mass spectrometry	502:540	liquid chromatography mass spectrometry	502:540	Methods Unheated e-cigarette liquids were analyzed using liquid chromatography mass spectrometry and enzymatic test kits.					
29182761	10	7	theme	significant	1574:1584	arg1	correlations					1586:1597	no significant correlations	1571:1597	no significant correlations between acetaldehyde and any of the sugars or any of the aldehydes and glucose	1571:1676	There were no significant correlations between acetaldehyde and any of the sugars or any of the aldehydes and glucose.					
29182761	12	8	theme	young	1990:1994	arg1	people					1996:2001	young people	1990:2001	young people	1990:2001	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	8	9	theme	Acetaldehyde	1088:1099	arg1	levels					1101:1106	Acetaldehyde levels	1088:1106	Acetaldehyde levels	1088:1106	Acetaldehyde levels were significantly higher than formaldehyde [χ2(1) = 11.7, p = .0006] and acrolein [χ2(1) = 119.5, p < .0001] levels.					
29182761	3	10	theme	enzymatic	546:554	arg1	kits					561:564	enzymatic test kits	546:564	enzymatic test kits	546:564	Methods Unheated e-cigarette liquids were analyzed using liquid chromatography mass spectrometry and enzymatic test kits.					
29182761	13	11	theme	FDA	2301:2303	arg1	policies					2316:2323	future FDA regulatory policies	2294:2323	future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids	2294:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	8	12	theme	p	1207:1207	arg1	<					1209:1209	p < .0001	1207:1215	p < .0001	1207:1215	Acetaldehyde levels were significantly higher than formaldehyde [χ2(1) = 11.7, p = .0006] and acrolein [χ2(1) = 119.5, p < .0001] levels.					
29182761	4	13	theme	nicotine	683:690	arg1	concentration					692:704	nicotine concentration	683:704	nicotine concentration	683:704	Generalized linear models, Fisher's exact test, and Pearson's correlation coefficient assessed sugar, aldehyde, and nicotine concentration associations.					
29182761	1	14	from	content	191:197	arg1	liquids					298:304	electronic cigarette (e-cigarette) liquids	263:304	electronic cigarette (e-cigarette) liquids	263:304	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	6	15	theme	glucose	885:891	arg1	[χ2					893:895	glucose [χ2(1)	885:898	glucose [χ2(1) = 85.9	885:905	Sucrose levels were significantly higher than glucose [χ2(1) = 85.9, p < .0001] and fructose [χ2(1) = 10.6, p = .001] levels.					
29182761	8	16	theme	formaldehyde	1139:1150	arg1	[χ2					1152:1154	formaldehyde [χ2(1)	1139:1157	formaldehyde [χ2(1) = 11.7, p = .0006] and acrolein [χ2(1) = 119.5, p < .0001] levels	1139:1223	Acetaldehyde levels were significantly higher than formaldehyde [χ2(1) = 11.7, p = .0006] and acrolein [χ2(1) = 119.5, p < .0001] levels.					
29182761	9	17	theme	labeled	1279:1285	arg1	liquids					1299:1305	nicotine-based and zero-nicotine labeled e-cigarette liquids	1246:1305	nicotine-based and zero-nicotine labeled e-cigarette liquids	1246:1305	Differences between nicotine-based and zero-nicotine labeled e-cigarette liquids were not statistically significant for sugars or aldehydes.					
29182761	1	18	theme	traditional	129:139	arg1	products					149:156	traditional tobacco products	129:156	traditional tobacco products	129:156	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	6	19	dep	p	908:908	arg1	.0001					912:916	.0001	912:916	.0001	912:916	Sucrose levels were significantly higher than glucose [χ2(1) = 85.9, p < .0001] and fructose [χ2(1) = 10.6, p = .001] levels.					
29182761	12	20	theme	specific	1916:1923	arg1	constituents					1932:1943	specific flavor constituents	1916:1943	specific flavor constituents	1916:1943	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	12	21	from	regulation	1902:1911	arg1	products					1956:1963	tobacco products	1948:1963	tobacco products	1948:1963	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	0	22	from	Sugar	0:4	arg1	Liquids					60:66	Flavored Electronic Cigarette Liquids	30:66	Flavored Electronic Cigarette Liquids	30:66	Sugar and Aldehyde Content in Flavored Electronic Cigarette Liquids.					
29182761	11	23	theme	e-cigarette	1748:1758	arg1	liquids					1760:1766	unheated e-cigarette liquids	1739:1766	unheated e-cigarette liquids	1739:1766	Conclusions Sugars and related aldehydes were identified in unheated e-cigarette liquids and their composition may influence experimentation in naïve users and their potential toxicity.					
29182761	12	24	theme	constituents	1932:1943	arg1	regulation					1902:1911	the regulation	1898:1911	the regulation of specific flavor constituents in tobacco products	1898:1963	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	12	24	theme	constituents	1932:1943	arg1	strategy					1970:1977	a strategy	1968:1977	a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking	1968:2177	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	3	25	dep	Methods	445:451	arg1	liquids					474:480	Unheated e-cigarette liquids	453:480	Methods Unheated e-cigarette liquids	445:480	Methods Unheated e-cigarette liquids were analyzed using liquid chromatography mass spectrometry and enzymatic test kits.					
29182761	1	26	theme	tobacco	141:147	arg1	products					149:156	traditional tobacco products	129:156	traditional tobacco products	129:156	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	6	27	dep	higher	873:878	arg1	=					939:939	=	939:939	= 10.6, p = .001] levels	939:962	Sucrose levels were significantly higher than glucose [χ2(1) = 85.9, p < .0001] and fructose [χ2(1) = 10.6, p = .001] levels.					
29182761	6	28	theme	=	949:949	arg1	p					947:947	p = .001	947:954	p = .001	947:954	Sucrose levels were significantly higher than glucose [χ2(1) = 85.9, p < .0001] and fructose [χ2(1) = 10.6, p = .001] levels.					
29182761	2	29	theme	e-cigarette	363:373	arg1	liquids					375:381	e-cigarette liquids	363:381	e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66)	363:442	This study quantified levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66).					
29182761	1	30	from	additives	116:124	arg1	products					149:156	traditional tobacco products	129:156	traditional tobacco products	129:156	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	1	31	theme	major	93:97	arg1	Sugars					82:87	Introduction Sugars	69:87	Introduction Sugars	69:87	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	1	31	theme	major	93:97	arg1	constituents					99:110	major constituents	93:110	major constituents	93:110	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	2	32	theme	nicotine	411:418	arg1	concentrations					420:433	nicotine concentrations	411:433	nicotine concentrations (n = 66)	411:442	This study quantified levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66).					
29182761	2	32	theme	nicotine	411:418	arg1	n					436:436	n = 66	436:441	n = 66	436:441	This study quantified levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66).					
29182761	0	33	theme	Aldehyde	10:17	arg1	Content					19:25	Aldehyde Content	10:25	Aldehyde Content	10:25	Sugar and Aldehyde Content in Flavored Electronic Cigarette Liquids.					
29182761	1	34	theme	cigarette	274:282	arg1	liquids					298:304	electronic cigarette (e-cigarette) liquids	263:304	electronic cigarette (e-cigarette) liquids	263:304	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	12	35	theme	emerging	2072:2079	arg1	products					2081:2088	these emerging products	2066:2088	these emerging products	2066:2088	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	9	36	theme	e-cigarette	1287:1297	arg1	liquids					1299:1305	nicotine-based and zero-nicotine labeled e-cigarette liquids	1246:1305	nicotine-based and zero-nicotine labeled e-cigarette liquids	1246:1305	Differences between nicotine-based and zero-nicotine labeled e-cigarette liquids were not statistically significant for sugars or aldehydes.					
29182761	13	37	theme	regulatory	2305:2314	arg1	policies					2316:2323	future FDA regulatory policies	2294:2323	future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids	2294:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	11	38	theme	Conclusions	1679:1689	arg1	Sugars					1691:1696	Conclusions Sugars	1679:1696	Conclusions Sugars	1679:1696	Conclusions Sugars and related aldehydes were identified in unheated e-cigarette liquids and their composition may influence experimentation in naïve users and their potential toxicity.					
29182761	6	39	dep	levels	957:962	arg1	p					947:947	p = .001	947:954	p = .001	947:954	Sucrose levels were significantly higher than glucose [χ2(1) = 85.9, p < .0001] and fructose [χ2(1) = 10.6, p = .001] levels.					
29182761	1	40	theme	e-cigarette	285:295	arg1	liquids					298:304	electronic cigarette (e-cigarette) liquids	263:304	electronic cigarette (e-cigarette) liquids	263:304	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	2	41	theme	=	438:438	arg1	concentrations					420:433	nicotine concentrations	411:433	nicotine concentrations (n = 66)	411:442	This study quantified levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66).					
29182761	2	41	theme	=	438:438	arg1	n					436:436	n = 66	436:441	n = 66	436:441	This study quantified levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66).					
29182761	3	42	theme	Unheated	453:460	arg1	liquids					474:480	Unheated e-cigarette liquids	453:480	Methods Unheated e-cigarette liquids	445:480	Methods Unheated e-cigarette liquids were analyzed using liquid chromatography mass spectrometry and enzymatic test kits.					
29182761	0	43	theme	Electronic	39:48	arg1	Liquids					60:66	Flavored Electronic Cigarette Liquids	30:66	Flavored Electronic Cigarette Liquids	30:66	Sugar and Aldehyde Content in Flavored Electronic Cigarette Liquids.					
29182761	13	44	theme	liquids	2410:2416	arg1	labeling					2386:2393	accurate and comprehensible labeling	2358:2393	accurate and comprehensible labeling of e-cigarette liquids	2358:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	13	44	theme	liquids	2410:2416	arg1	standards					2344:2352	product standards	2336:2352	product standards	2336:2352	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	13	45	theme	accurate	2358:2365	arg1	labeling					2386:2393	accurate and comprehensible labeling	2358:2393	accurate and comprehensible labeling of e-cigarette liquids	2358:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	1	46	dep	content	191:197	arg1	acetaldehyde					232:243	acetaldehyde	232:243	acetaldehyde	232:243	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	1	46	dep	content	191:197	arg1	acrolein					250:257	acrolein	250:257	acrolein	250:257	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	1	46	dep	content	191:197	arg1	formaldehyde					218:229	formaldehyde	218:229	formaldehyde	218:229	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	2	47	theme	aldehydes	350:358	arg1	levels					329:334	levels	329:334	levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66)	329:442	This study quantified levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66).					
29182761	0	48	theme	Cigarette	50:58	arg1	Liquids					60:66	Flavored Electronic Cigarette Liquids	30:66	Flavored Electronic Cigarette Liquids	30:66	Sugar and Aldehyde Content in Flavored Electronic Cigarette Liquids.					
29182761	13	49	theme	comprehensible	2371:2384	arg1	labeling					2386:2393	accurate and comprehensible labeling	2358:2393	accurate and comprehensible labeling of e-cigarette liquids	2358:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	7	50	theme	samples	1079:1085	arg1	%					1070:1070	75%	1068:1070	75%	1068:1070	Formaldehyde, acetaldehyde, and acrolein levels exceeded the limits of quantification in 72%, 84%, and 75% of the samples.					
29182761	7	50	theme	samples	1079:1085	arg1	samples					1079:1085	the samples	1075:1085	the samples	1075:1085	Formaldehyde, acetaldehyde, and acrolein levels exceeded the limits of quantification in 72%, 84%, and 75% of the samples.					
29182761	7	50	theme	samples	1079:1085	arg1	%					1056:1056	72%	1054:1056	72%	1054:1056	Formaldehyde, acetaldehyde, and acrolein levels exceeded the limits of quantification in 72%, 84%, and 75% of the samples.					
29182761	7	50	theme	samples	1079:1085	arg1	%					1061:1061	84%	1059:1061	84%	1059:1061	Formaldehyde, acetaldehyde, and acrolein levels exceeded the limits of quantification in 72%, 84%, and 75% of the samples.					
29182761	2	51	theme	sugars	339:344	arg1	levels					329:334	levels	329:334	levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66)	329:442	This study quantified levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66).					
29182761	3	52	theme	mass	524:527	arg1	spectrometry					529:540	liquid chromatography mass spectrometry	502:540	liquid chromatography mass spectrometry	502:540	Methods Unheated e-cigarette liquids were analyzed using liquid chromatography mass spectrometry and enzymatic test kits.					
29182761	2	53	from	levels	329:334	arg1	liquids					375:381	e-cigarette liquids	363:381	e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66)	363:442	This study quantified levels of sugars and aldehydes in e-cigarette liquids across brands, flavors, and nicotine concentrations (n = 66).					
29182761	4	54	theme	linear	579:584	arg1	models					586:591	Generalized linear models	567:591	Generalized linear models	567:591	Generalized linear models, Fisher's exact test, and Pearson's correlation coefficient assessed sugar, aldehyde, and nicotine concentration associations.					
29182761	3	55	theme	liquid	502:507	arg1	spectrometry					529:540	liquid chromatography mass spectrometry	502:540	liquid chromatography mass spectrometry	502:540	Methods Unheated e-cigarette liquids were analyzed using liquid chromatography mass spectrometry and enzymatic test kits.					
29182761	13	56	theme	related	2325:2331	arg1	policies					2316:2323	future FDA regulatory policies	2294:2323	future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids	2294:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	3	57	theme	test	556:559	arg1	kits					561:564	enzymatic test kits	546:564	enzymatic test kits	546:564	Methods Unheated e-cigarette liquids were analyzed using liquid chromatography mass spectrometry and enzymatic test kits.					
29182761	13	58	theme	product	2336:2342	arg1	standards					2344:2352	product standards	2336:2352	product standards	2336:2352	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	13	59	used	used	2201:2204	arg2	data					2184:2187	The data	2180:2187	The data	2180:2187	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	13	60	theme	future	2294:2299	arg1	policies					2316:2323	future FDA regulatory policies	2294:2323	future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids	2294:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	1	61	theme	related	202:208	arg1	toxins					210:215	related toxins	202:215	related toxins	202:215	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	12	62	theme	tobacco	1948:1954	arg1	products					1956:1963	tobacco products	1948:1963	tobacco products	1948:1963	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	12	63	theme	adult	2116:2120	arg1	smokers					2122:2128	adult smokers	2116:2128	adult smokers who are seeking to safely quit cigarette smoking	2116:2177	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	1	64	from	toxins	210:215	arg1	liquids					298:304	electronic cigarette (e-cigarette) liquids	263:304	electronic cigarette (e-cigarette) liquids	263:304	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	9	65	theme	nicotine-based	1246:1259	arg1	liquids					1299:1305	nicotine-based and zero-nicotine labeled e-cigarette liquids	1246:1305	nicotine-based and zero-nicotine labeled e-cigarette liquids	1246:1305	Differences between nicotine-based and zero-nicotine labeled e-cigarette liquids were not statistically significant for sugars or aldehydes.					
29182761	11	66	theme	naïve	1823:1827	arg1	users					1829:1833	naïve users	1823:1833	naïve users	1823:1833	Conclusions Sugars and related aldehydes were identified in unheated e-cigarette liquids and their composition may influence experimentation in naïve users and their potential toxicity.					
29182761	9	67	theme	zero-nicotine	1265:1277	arg1	liquids					1299:1305	nicotine-based and zero-nicotine labeled e-cigarette liquids	1246:1305	nicotine-based and zero-nicotine labeled e-cigarette liquids	1246:1305	Differences between nicotine-based and zero-nicotine labeled e-cigarette liquids were not statistically significant for sugars or aldehydes.					
29182761	5	68	dep	Results	720:726	arg1	levels					758:763	Glucose, fructose and sucrose levels	728:763	Results Glucose, fructose and sucrose levels	720:763	Results Glucose, fructose and sucrose levels exceeded the limits of quantification in 22%, 53% and 53% of the samples.					
29182761	4	69	theme	concentration	692:704	arg1	associations					706:717	sugar, aldehyde, and nicotine concentration associations	662:717	sugar, aldehyde, and nicotine concentration associations	662:717	Generalized linear models, Fisher's exact test, and Pearson's correlation coefficient assessed sugar, aldehyde, and nicotine concentration associations.					
29182761	11	70	theme	unheated	1739:1746	arg1	liquids					1760:1766	unheated e-cigarette liquids	1739:1766	unheated e-cigarette liquids	1739:1766	Conclusions Sugars and related aldehydes were identified in unheated e-cigarette liquids and their composition may influence experimentation in naïve users and their potential toxicity.					
29182761	5	71	theme	fructose	737:744	arg1	levels					758:763	Glucose, fructose and sucrose levels	728:763	Results Glucose, fructose and sucrose levels	720:763	Results Glucose, fructose and sucrose levels exceeded the limits of quantification in 22%, 53% and 53% of the samples.					
29182761	5	72	theme	samples	830:836	arg1	%					813:813	53%	811:813	53%	811:813	Results Glucose, fructose and sucrose levels exceeded the limits of quantification in 22%, 53% and 53% of the samples.					
29182761	5	72	theme	samples	830:836	arg1	%					821:821	53%	819:821	53%	819:821	Results Glucose, fructose and sucrose levels exceeded the limits of quantification in 22%, 53% and 53% of the samples.					
29182761	5	72	theme	samples	830:836	arg1	samples					830:836	the samples	826:836	the samples	826:836	Results Glucose, fructose and sucrose levels exceeded the limits of quantification in 22%, 53% and 53% of the samples.					
29182761	5	72	theme	samples	830:836	arg1	%					808:808	22%	806:808	22%	806:808	Results Glucose, fructose and sucrose levels exceeded the limits of quantification in 22%, 53% and 53% of the samples.					
29182761	6	73	theme	fructose	923:930	arg1	1					936:936	1	936:936	1	936:936	Sucrose levels were significantly higher than glucose [χ2(1) = 85.9, p < .0001] and fructose [χ2(1) = 10.6, p = .001] levels.					
29182761	6	73	theme	fructose	923:930	arg1	[χ2					932:934	fructose [χ2	923:934	fructose [χ2(1)	923:937	Sucrose levels were significantly higher than glucose [χ2(1) = 85.9, p < .0001] and fructose [χ2(1) = 10.6, p = .001] levels.					
29182761	0	74	from	Content	19:25	arg1	Liquids					60:66	Flavored Electronic Cigarette Liquids	30:66	Flavored Electronic Cigarette Liquids	30:66	Sugar and Aldehyde Content in Flavored Electronic Cigarette Liquids.					
29182761	4	75	theme	sugar	662:666	arg1	associations					706:717	sugar, aldehyde, and nicotine concentration associations	662:717	sugar, aldehyde, and nicotine concentration associations	662:717	Generalized linear models, Fisher's exact test, and Pearson's correlation coefficient assessed sugar, aldehyde, and nicotine concentration associations.					
29182761	5	76	theme	sucrose	750:756	arg1	levels					758:763	Glucose, fructose and sucrose levels	728:763	Results Glucose, fructose and sucrose levels	720:763	Results Glucose, fructose and sucrose levels exceeded the limits of quantification in 22%, 53% and 53% of the samples.					
29182761	12	77	theme	flavor	1925:1930	arg1	constituents					1932:1943	specific flavor constituents	1916:1943	specific flavor constituents	1916:1943	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	4	78	theme	aldehyde	669:676	arg1	associations					706:717	sugar, aldehyde, and nicotine concentration associations	662:717	sugar, aldehyde, and nicotine concentration associations	662:717	Generalized linear models, Fisher's exact test, and Pearson's correlation coefficient assessed sugar, aldehyde, and nicotine concentration associations.					
29182761	5	79	theme	Glucose	728:734	arg1	levels					758:763	Glucose, fructose and sucrose levels	728:763	Results Glucose, fructose and sucrose levels	720:763	Results Glucose, fructose and sucrose levels exceeded the limits of quantification in 22%, 53% and 53% of the samples.					
29182761	1	80	theme	Introduction	69:80	arg1	Sugars					82:87	Introduction Sugars	69:87	Introduction Sugars	69:87	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	1	80	theme	Introduction	69:80	arg1	constituents					99:110	major constituents	93:110	major constituents	93:110	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	13	81	from	ingredients	2233:2243	arg1	products					2248:2255	products	2248:2255	products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids	2248:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	5	82	theme	quantification	788:801	arg1	limits					778:783	the limits	774:783	the limits of quantification	774:801	Results Glucose, fructose and sucrose levels exceeded the limits of quantification in 22%, 53% and 53% of the samples.					
29182761	8	83	theme	=	1169:1169	arg1	p					1167:1167	p = .0006	1167:1175	p = .0006	1167:1175	Acetaldehyde levels were significantly higher than formaldehyde [χ2(1) = 11.7, p = .0006] and acrolein [χ2(1) = 119.5, p < .0001] levels.					
29182761	1	84	theme	electronic	263:272	arg1	liquids					298:304	electronic cigarette (e-cigarette) liquids	263:304	electronic cigarette (e-cigarette) liquids	263:304	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	13	85	contain	contain	2266:2272	arg2	nicotine					2274:2281	nicotine	2274:2281	nicotine	2274:2281	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	13	85	contain	contain	2266:2272	arg1	products					2248:2255	products	2248:2255	products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids	2248:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	0	86	theme	Flavored	30:37	arg1	Liquids					60:66	Flavored Electronic Cigarette Liquids	30:66	Flavored Electronic Cigarette Liquids	30:66	Sugar and Aldehyde Content in Flavored Electronic Cigarette Liquids.					
29182761	8	87	dep	levels	1218:1223	arg1	<					1209:1209	p < .0001	1207:1215	p < .0001	1207:1215	Acetaldehyde levels were significantly higher than formaldehyde [χ2(1) = 11.7, p = .0006] and acrolein [χ2(1) = 119.5, p < .0001] levels.					
29182761	8	88	dep	11.7	1161:1164	arg1	p					1167:1167	p = .0006	1167:1175	p = .0006	1167:1175	Acetaldehyde levels were significantly higher than formaldehyde [χ2(1) = 11.7, p = .0006] and acrolein [χ2(1) = 119.5, p < .0001] levels.					
29182761	7	89	theme	acrolein	997:1004	arg1	levels					1006:1011	Formaldehyde, acetaldehyde, and acrolein levels	965:1011	Formaldehyde, acetaldehyde, and acrolein levels	965:1011	Formaldehyde, acetaldehyde, and acrolein levels exceeded the limits of quantification in 72%, 84%, and 75% of the samples.					
29182761	13	90	theme	e-cigarette	2398:2408	arg1	liquids					2410:2416	e-cigarette liquids	2398:2416	e-cigarette liquids	2398:2416	The data can also be used to educate consumers about ingredients in products that may contain nicotine and inform future FDA regulatory policies related to product standards and accurate and comprehensible labeling of e-cigarette liquids.					
29182761	11	91	theme	related	1702:1708	arg1	aldehydes					1710:1718	related aldehydes	1702:1718	related aldehydes	1702:1718	Conclusions Sugars and related aldehydes were identified in unheated e-cigarette liquids and their composition may influence experimentation in naïve users and their potential toxicity.					
29182761	3	92	theme	e-cigarette	462:472	arg1	liquids					474:480	Unheated e-cigarette liquids	453:480	Methods Unheated e-cigarette liquids	445:480	Methods Unheated e-cigarette liquids were analyzed using liquid chromatography mass spectrometry and enzymatic test kits.					
29182761	1	93	from	constituents	99:110	arg1	products					149:156	traditional tobacco products	129:156	traditional tobacco products	129:156	Introduction Sugars are major constituents and additives in traditional tobacco products, but little is known about their content or related toxins (formaldehyde, acetaldehyde, and acrolein) in electronic cigarette (e-cigarette) liquids.					
29182761	11	94	theme	potential	1845:1853	arg1	toxicity					1855:1862	their potential toxicity	1839:1862	their potential toxicity	1839:1862	Conclusions Sugars and related aldehydes were identified in unheated e-cigarette liquids and their composition may influence experimentation in naïve users and their potential toxicity.					
29182761	4	95	theme	exact	603:607	arg1	test					609:612	Fisher's exact test	594:612	Fisher's exact test	594:612	Generalized linear models, Fisher's exact test, and Pearson's correlation coefficient assessed sugar, aldehyde, and nicotine concentration associations.					
29182761	12	96	theme	cigarette	2161:2169	arg1	smoking					2171:2177	cigarette smoking	2161:2177	cigarette smoking	2161:2177	Implications The data can inform the regulation of specific flavor constituents in tobacco products as a strategy to protect young people from using e-cigarettes, while balancing FDA's interest in how these emerging products could potentially benefit adult smokers who are seeking to safely quit cigarette smoking.					
29182761	7	97	theme	quantification	1036:1049	arg1	limits					1026:1031	the limits	1022:1031	the limits of quantification	1022:1049	Formaldehyde, acetaldehyde, and acrolein levels exceeded the limits of quantification in 72%, 84%, and 75% of the samples.					
31054459	5	0	from	adsorption	825:834	arg1	surface					873:879	magnetite nanoparticles surface	849:879	magnetite nanoparticles surface	849:879	FTIR spectra confirmed the adsorption of pectin on magnetite nanoparticles surface.					
31054459	13	1	theme	simulated	1830:1838	arg1	fluids					1840:1845	simulated fluids	1830:1845	simulated fluids	1830:1845	Regarding bacterial viability, no decays were observed neither when pectin-decorated nanoparticles were exposed to simulated fluids nor when stored at 4 °C for 60 days.					
31054459	10	2	theme	partial	1430:1436	arg1	dissolution					1438:1448	partial dissolution	1430:1448	partial dissolution	1430:1448	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	1	3	theme	delivery	256:263	arg1	them					248:251	them	248:251	them	248:251	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	1	3	theme	delivery	256:263	arg1	systems					265:271	delivery systems	256:271	delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114	256:332	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	5	4	theme	FTIR	798:801	arg1	spectra					803:809	FTIR spectra	798:809	FTIR spectra	798:809	FTIR spectra confirmed the adsorption of pectin on magnetite nanoparticles surface.					
31054459	6	5	theme	morphological	945:957	arg1	change					967:972	a morphological surface change	943:972	a morphological surface change	943:972	SEM and TEM images evidenced agglomerated nanoparticles, and a morphological surface change after adsorption of pectin.					
31054459	8	6	dep	1.00 mg/mL	1229:1238	arg1	up					1223:1224	up	1223:1224	up	1223:1224	Iron from nanoparticles demonstrated to be non-toxic for microorganisms up to 1.00 mg/mL.					
31054459	9	7	theme	Simulated	1241:1249	arg1	saliva					1251:1256	Simulated saliva	1241:1256	Simulated saliva	1241:1256	Simulated saliva and gastric solutions prevented nanoparticles from dissolution.					
31054459	1	8	theme	biophysical	160:170	arg1	stability					172:180	biophysical stability	160:180	biophysical stability of iron-pectin nanoparticles	160:209	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	12	9	theme	soluble	1615:1621	arg1	species					1623:1629	The soluble species	1611:1629	The soluble species	1611:1629	The soluble species diffused out from the aggregates, being detected in the simulated intestinal fluid.					
31054459	10	10	dep	conditions	1354:1363	arg1	-COO-					1375:1379	solvated -COO- and Fe-O- groups	1366:1396	-COO-	1375:1379	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	10	10	dep	conditions	1354:1363	arg1	groups					1391:1396	solvated -COO- and Fe-O- groups	1366:1396	groups	1391:1396	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	7	11	theme	ζ-potential	1010:1020	arg1	results					1022:1028	ζ-potential results	1010:1028	ζ-potential results	1010:1028	DLS and ζ-potential results proved the solvation of the ionizable groups in the hydrophilic network which induced chain expansion and agglomeration.					
31054459	4	12	theme	phases	790:795	arg1	presence					756:763	the almost exclusive presence	735:763	the almost exclusive presence of magnetite crystalline phases	735:795	XRD indicated the almost exclusive presence of magnetite crystalline phases.					
31054459	8	13	from	nanoparticles	1161:1173	arg1	Iron					1151:1154	Iron	1151:1154	Iron from nanoparticles	1151:1173	Iron from nanoparticles demonstrated to be non-toxic for microorganisms up to 1.00 mg/mL.					
31054459	0	14	theme	probiotic	99:107	arg1	bacteria					109:116	probiotic bacteria	99:116	probiotic bacteria	99:116	Pectin-decorated magnetite nanoparticles as both iron delivery systems and protective matrices for probiotic bacteria.					
31054459	4	15	theme	crystalline	778:788	arg1	phases					790:795	magnetite crystalline phases	768:795	magnetite crystalline phases	768:795	XRD indicated the almost exclusive presence of magnetite crystalline phases.					
31054459	10	16	theme	Fe-O-	1385:1389	arg1	groups					1391:1396	solvated -COO- and Fe-O- groups	1366:1396	groups	1391:1396	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	2	17	theme	oxide	340:344	arg1	nanoparticles					354:366	Iron oxide (Fe3O4) nanoparticles	335:366	Iron oxide (Fe3O4) nanoparticles	335:366	Iron oxide (Fe3O4) nanoparticles were synthesized from 0.25M FeCl2/0.5 M FeCl3.6H2O, and coated with citrus pectins.					
31054459	11	18	theme	magnetite	1490:1498	arg1	nanoparticles					1500:1512	magnetite nanoparticles	1490:1512	magnetite nanoparticles	1490:1512	Pectins adsorption on magnetite nanoparticles significantly enhanced electrostatic repulsion, which aided the solvation of ionized iron forms.					
31054459	9	19	theme	gastric	1262:1268	arg1	solutions					1270:1278	gastric solutions	1262:1278	gastric solutions	1262:1278	Simulated saliva and gastric solutions prevented nanoparticles from dissolution.					
31054459	13	20	theme	bacterial	1725:1733	arg1	viability					1735:1743	bacterial viability	1725:1743	bacterial viability	1725:1743	Regarding bacterial viability, no decays were observed neither when pectin-decorated nanoparticles were exposed to simulated fluids nor when stored at 4 °C for 60 days.					
31054459	10	21	theme	solvated	1366:1373	arg1	-COO-					1375:1379	solvated -COO- and Fe-O- groups	1366:1396	-COO-	1375:1379	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	7	22	from	solvation	1041:1049	arg1	network					1094:1100	the hydrophilic network	1078:1100	the hydrophilic network which induced chain expansion and agglomeration	1078:1148	DLS and ζ-potential results proved the solvation of the ionizable groups in the hydrophilic network which induced chain expansion and agglomeration.					
31054459	5	23	theme	pectin	839:844	arg1	adsorption					825:834	the adsorption	821:834	the adsorption of pectin on magnetite nanoparticles surface	821:879	FTIR spectra confirmed the adsorption of pectin on magnetite nanoparticles surface.					
31054459	11	24	theme	electrostatic	1537:1549	arg1	repulsion					1551:1559	electrostatic repulsion	1537:1559	electrostatic repulsion	1537:1559	Pectins adsorption on magnetite nanoparticles significantly enhanced electrostatic repulsion, which aided the solvation of ionized iron forms.					
31054459	3	25	theme	physico-chemical	458:473	arg1	properties					475:484	Their physico-chemical properties	452:484	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM]	452:554	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	11	26	theme	Pectins	1468:1474	arg1	adsorption					1476:1485	Pectins adsorption	1468:1485	Pectins adsorption on magnetite nanoparticles	1468:1512	Pectins adsorption on magnetite nanoparticles significantly enhanced electrostatic repulsion, which aided the solvation of ionized iron forms.					
31054459	14	27	theme	adequate	1933:1940	arg1	systems					1951:1957	adequate delivery systems	1933:1957	adequate delivery systems for probiotic bacteria, whose target is the gut	1933:2005	The composites engineered in this work appear as adequate delivery systems for probiotic bacteria, whose target is the gut.					
31054459	2	28	theme	Iron	335:338	arg1	Fe3O4					347:351	Fe3O4	347:351	Fe3O4	347:351	Iron oxide (Fe3O4) nanoparticles were synthesized from 0.25M FeCl2/0.5 M FeCl3.6H2O, and coated with citrus pectins.					
31054459	2	28	theme	Iron	335:338	arg1	oxide					340:344	Iron oxide	335:344	Iron oxide (Fe3O4) nanoparticles	335:366	Iron oxide (Fe3O4) nanoparticles were synthesized from 0.25M FeCl2/0.5 M FeCl3.6H2O, and coated with citrus pectins.					
31054459	10	29	theme	conditions	1354:1363	arg1	pH					1333:1334	The higher pH	1322:1334	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups)	1322:1397	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	1	30	theme	iron-pectin	185:195	arg1	nanoparticles					197:209	iron-pectin nanoparticles	185:209	iron-pectin nanoparticles	185:209	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	0	31	theme	Pectin-decorated	0:15	arg1	magnetite					17:25	Pectin-decorated magnetite	0:25	Pectin-decorated magnetite	0:25	Pectin-decorated magnetite nanoparticles as both iron delivery systems and protective matrices for probiotic bacteria.					
31054459	1	32	theme	probiotic	281:289	arg1	83114					328:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	4	33	theme	exclusive	746:754	arg1	presence					756:763	the almost exclusive presence	735:763	the almost exclusive presence of magnetite crystalline phases	735:795	XRD indicated the almost exclusive presence of magnetite crystalline phases.					
31054459	3	34	from	effect	566:571	arg1	stabilization					586:598	bacterial stabilization	576:598	bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions)	576:704	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	1	35	theme	nanoparticles	197:209	arg1	stability					172:180	biophysical stability	160:180	biophysical stability of iron-pectin nanoparticles	160:209	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	1	36	theme	strain	291:296	arg1	83114					328:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	7	37	theme	chain	1116:1120	arg1	expansion					1122:1130	chain expansion	1116:1130	chain expansion	1116:1130	DLS and ζ-potential results proved the solvation of the ionizable groups in the hydrophilic network which induced chain expansion and agglomeration.					
31054459	4	38	theme	magnetite	768:776	arg1	phases					790:795	magnetite crystalline phases	768:795	magnetite crystalline phases	768:795	XRD indicated the almost exclusive presence of magnetite crystalline phases.					
31054459	2	39	theme	citrus	436:441	arg1	pectins					443:449	citrus pectins	436:449	citrus pectins	436:449	Iron oxide (Fe3O4) nanoparticles were synthesized from 0.25M FeCl2/0.5 M FeCl3.6H2O, and coated with citrus pectins.					
31054459	5	40	theme	magnetite	849:857	arg1	surface					873:879	magnetite nanoparticles surface	849:879	magnetite nanoparticles surface	849:879	FTIR spectra confirmed the adsorption of pectin on magnetite nanoparticles surface.					
31054459	1	41	theme	Lactobacillus	298:310	arg1	83114					328:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	6	42	theme	SEM	882:884	arg1	images					894:899	SEM and TEM images	882:899	SEM and TEM images evidenced	882:909	SEM and TEM images evidenced agglomerated nanoparticles, and a morphological surface change after adsorption of pectin.					
31054459	10	43	dep	dispersion	1415:1424	arg1	the					1411:1413	the	1411:1413	the	1411:1413	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	6	44	theme	surface	959:965	arg1	change					967:972	a morphological surface change	943:972	a morphological surface change	943:972	SEM and TEM images evidenced agglomerated nanoparticles, and a morphological surface change after adsorption of pectin.					
31054459	3	45	theme	X-ray	493:497	arg1	XRD					512:514	XRD	512:514	XRD	512:514	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	45	theme	X-ray	493:497	arg1	[FTIR					486:490	[FTIR	486:490	[FTIR	486:490	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	45	theme	X-ray	493:497	arg1	diffraction					499:509	X-ray diffraction	493:509	X-ray diffraction (XRD)	493:515	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	0	46	theme	iron	49:52	arg1	systems					63:69	iron delivery systems	49:69	iron delivery systems	49:69	Pectin-decorated magnetite nanoparticles as both iron delivery systems and protective matrices for probiotic bacteria.					
31054459	1	47	theme	plantarum	312:320	arg1	83114					328:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	3	48	theme	bacterial	576:584	arg1	stabilization					586:598	bacterial stabilization	576:598	bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions)	576:704	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	49	dep	stabilization	586:598	arg1	viability					601:609	viability	601:609	viability after freeze-drying/storage	601:637	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	49	dep	stabilization	586:598	arg1	stability					640:648	stability	640:648	stability when exposed to simulated gastro-intestinal conditions	640:703	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	11	50	theme	forms	1604:1608	arg1	solvation					1578:1586	the solvation	1574:1586	the solvation of ionized iron forms	1574:1608	Pectins adsorption on magnetite nanoparticles significantly enhanced electrostatic repulsion, which aided the solvation of ionized iron forms.					
31054459	1	51	theme	CIDCA	322:326	arg1	83114					328:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	the probiotic strain Lactobacillus plantarum CIDCA 83114	277:332	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	13	52	theme	pectin-decorated	1783:1798	arg1	nanoparticles					1800:1812	pectin-decorated nanoparticles	1783:1812	pectin-decorated nanoparticles	1783:1812	Regarding bacterial viability, no decays were observed neither when pectin-decorated nanoparticles were exposed to simulated fluids nor when stored at 4 °C for 60 days.					
31054459	6	53	theme	TEM	890:892	arg1	images					894:899	SEM and TEM images	882:899	SEM and TEM images evidenced	882:909	SEM and TEM images evidenced agglomerated nanoparticles, and a morphological surface change after adsorption of pectin.					
31054459	3	54	from	properties	475:484	arg1	stabilization					586:598	bacterial stabilization	576:598	bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions)	576:704	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	12	55	theme	simulated	1687:1695	arg1	fluid					1708:1712	the simulated intestinal fluid	1683:1712	the simulated intestinal fluid	1683:1712	The soluble species diffused out from the aggregates, being detected in the simulated intestinal fluid.					
31054459	6	56	theme	pectin	994:999	arg1	adsorption					980:989	adsorption	980:989	adsorption of pectin	980:999	SEM and TEM images evidenced agglomerated nanoparticles, and a morphological surface change after adsorption of pectin.					
31054459	7	57	theme	hydrophilic	1082:1092	arg1	network					1094:1100	the hydrophilic network	1078:1100	the hydrophilic network which induced chain expansion and agglomeration	1078:1148	DLS and ζ-potential results proved the solvation of the ionizable groups in the hydrophilic network which induced chain expansion and agglomeration.					
31054459	7	58	theme	groups	1068:1073	arg1	solvation					1041:1049	the solvation	1037:1049	the solvation of the ionizable groups in the hydrophilic network which induced chain expansion and agglomeration	1037:1148	DLS and ζ-potential results proved the solvation of the ionizable groups in the hydrophilic network which induced chain expansion and agglomeration.					
31054459	0	59	theme	delivery	54:61	arg1	systems					63:69	iron delivery systems	49:69	iron delivery systems	49:69	Pectin-decorated magnetite nanoparticles as both iron delivery systems and protective matrices for probiotic bacteria.					
31054459	3	60	theme	gastro-intestinal	676:692	arg1	conditions					694:703	simulated gastro-intestinal conditions	666:703	simulated gastro-intestinal conditions	666:703	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	10	61	theme	higher	1326:1331	arg1	pH					1333:1334	The higher pH	1322:1334	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups)	1322:1397	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	12	62	theme	intestinal	1697:1706	arg1	fluid					1708:1712	the simulated intestinal fluid	1683:1712	the simulated intestinal fluid	1683:1712	The soluble species diffused out from the aggregates, being detected in the simulated intestinal fluid.					
31054459	3	63	dep	properties	475:484	arg1	ζ-potential					518:528	ζ-potential	518:528	ζ-potential	518:528	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	63	dep	properties	475:484	arg1	TEM					551:553	TEM	551:553	TEM	551:553	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	63	dep	properties	475:484	arg1	diffraction					499:509	X-ray diffraction	493:509	X-ray diffraction (XRD)	493:515	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	63	dep	properties	475:484	arg1	size					540:543	particle size	531:543	particle size	531:543	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	63	dep	properties	475:484	arg1	SEM					546:548	SEM	546:548	SEM	546:548	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	63	dep	properties	475:484	arg1	[FTIR					486:490	[FTIR	486:490	[FTIR	486:490	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	11	64	from	adsorption	1476:1485	arg1	nanoparticles					1500:1512	magnetite nanoparticles	1490:1512	magnetite nanoparticles	1490:1512	Pectins adsorption on magnetite nanoparticles significantly enhanced electrostatic repulsion, which aided the solvation of ionized iron forms.					
31054459	7	65	theme	ionizable	1058:1066	arg1	groups					1068:1073	the ionizable groups	1054:1073	the ionizable groups	1054:1073	DLS and ζ-potential results proved the solvation of the ionizable groups in the hydrophilic network which induced chain expansion and agglomeration.					
31054459	10	66	theme	nanoparticles	1453:1465	arg1	dispersion					1415:1424	dispersion	1415:1424	dispersion	1415:1424	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	10	66	theme	nanoparticles	1453:1465	arg1	dissolution					1438:1448	partial dissolution	1430:1448	partial dissolution	1430:1448	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31054459	1	67	theme	work	136:139	arg1	goal					123:126	The goal	119:126	The goal of this work	119:139	The goal of this work was to investigate biophysical stability of iron-pectin nanoparticles and analyze the feasibility of using them as delivery systems for the probiotic strain Lactobacillus plantarum CIDCA 83114.					
31054459	11	68	theme	ionized	1591:1597	arg1	forms					1604:1608	ionized iron forms	1591:1608	ionized iron forms	1591:1608	Pectins adsorption on magnetite nanoparticles significantly enhanced electrostatic repulsion, which aided the solvation of ionized iron forms.					
31054459	0	69	theme	protective	75:84	arg1	matrices					86:93	protective matrices	75:93	protective matrices	75:93	Pectin-decorated magnetite nanoparticles as both iron delivery systems and protective matrices for probiotic bacteria.					
31054459	3	70	theme	simulated	666:674	arg1	conditions					694:703	simulated gastro-intestinal conditions	666:703	simulated gastro-intestinal conditions	666:703	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	14	71	theme	delivery	1942:1949	arg1	systems					1951:1957	adequate delivery systems	1933:1957	adequate delivery systems for probiotic bacteria, whose target is the gut	1933:2005	The composites engineered in this work appear as adequate delivery systems for probiotic bacteria, whose target is the gut.					
31054459	5	72	theme	nanoparticles	859:871	arg1	surface					873:879	magnetite nanoparticles surface	849:879	magnetite nanoparticles surface	849:879	FTIR spectra confirmed the adsorption of pectin on magnetite nanoparticles surface.					
31054459	14	73	theme	probiotic	1963:1971	arg1	bacteria					1973:1980	probiotic bacteria	1963:1980	probiotic bacteria	1963:1980	The composites engineered in this work appear as adequate delivery systems for probiotic bacteria, whose target is the gut.					
31054459	11	74	theme	iron	1599:1602	arg1	forms					1604:1608	ionized iron forms	1591:1608	ionized iron forms	1591:1608	Pectins adsorption on magnetite nanoparticles significantly enhanced electrostatic repulsion, which aided the solvation of ionized iron forms.					
31054459	3	75	theme	particle	531:538	arg1	[FTIR					486:490	[FTIR	486:490	[FTIR	486:490	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	3	75	theme	particle	531:538	arg1	size					540:543	particle size	531:543	particle size	531:543	Their physico-chemical properties [FTIR, X-ray diffraction (XRD), ζ-potential, particle size, SEM, TEM] and their effect on bacterial stabilization (viability after freeze-drying/storage, stability when exposed to simulated gastro-intestinal conditions) were assessed.					
31054459	10	76	theme	intestinal	1343:1352	arg1	conditions					1354:1363	the intestinal conditions	1339:1363	the intestinal conditions (solvated -COO- and Fe-O- groups)	1339:1397	The higher pH of the intestinal conditions (solvated -COO- and Fe-O- groups) facilitated the dispersion and partial dissolution of nanoparticles.					
31000305	8	0	theme	frankfurter-type	1583:1598	arg1	sausages					1600:1607	nitrite-free frankfurter-type sausages	1570:1607	nitrite-free frankfurter-type sausages	1570:1607	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	3	1	theme	Chitosan + Mixed	643:658	arg1	sausages					668:675	1% Chitosan + Mixed Extract sausages	640:675	1% Chitosan + Mixed Extract sausages	640:675	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	1	2	theme	acid	386:389	arg1	composition					391:401	fatty acid composition	380:401	fatty acid composition	380:401	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	4	3	with	Combinations	846:857	arg1	Extract					906:912	Mixed Extract	900:912	Mixed Extract	900:912	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	7	4	theme	sausages	1348:1355	arg1	SFA					1307:1309	SFA	1307:1309	SFA	1307:1309	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	4	theme	sausages	1348:1355	arg1	acids					1300:1304	saturated, monounsaturated and polyunsaturated fatty acids	1247:1304	saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1247:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	4	theme	sausages	1348:1355	arg1	MUFA					1312:1315	MUFA	1312:1315	MUFA	1312:1315	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	4	theme	sausages	1348:1355	arg1	PUFA					1322:1325	PUFA	1322:1325	PUFA	1322:1325	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	3	5	theme	1	640:640	arg1	%					641:641	%	641:641	%	641:641	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	7	6	theme	acids	1300:1304	arg1	SFA					1307:1309	SFA	1307:1309	SFA	1307:1309	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	6	theme	acids	1300:1304	arg1	amounts					1236:1242	Total amounts	1230:1242	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1230:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	6	theme	acids	1300:1304	arg1	acids					1300:1304	saturated, monounsaturated and polyunsaturated fatty acids	1247:1304	saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1247:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	6	theme	acids	1300:1304	arg1	MUFA					1312:1315	MUFA	1312:1315	MUFA	1312:1315	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	6	theme	acids	1300:1304	arg1	PUFA					1322:1325	PUFA	1322:1325	PUFA	1322:1325	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	4	7	theme	%	865:865	arg1	ε-Polylysine					867:878	0.2% ε-Polylysine	862:878	0.2% ε-Polylysine	862:878	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	7	8	theme	polyunsaturated	1278:1292	arg1	SFA					1307:1309	SFA	1307:1309	SFA	1307:1309	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	8	theme	polyunsaturated	1278:1292	arg1	acids					1300:1304	saturated, monounsaturated and polyunsaturated fatty acids	1247:1304	saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1247:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	8	theme	polyunsaturated	1278:1292	arg1	MUFA					1312:1315	MUFA	1312:1315	MUFA	1312:1315	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	8	theme	polyunsaturated	1278:1292	arg1	PUFA					1322:1325	PUFA	1322:1325	PUFA	1322:1325	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	3	9	theme	reactive	718:725	arg1	TBARS					739:743	TBARS	739:743	TBARS	739:743	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	3	9	theme	reactive	718:725	arg1	substances					727:736	thiobarbituric acid reactive substances	698:736	thiobarbituric acid reactive substances (TBARS)	698:744	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	8	10	with	Combinations	1457:1468	arg1	Extract					1518:1524	Mixed Extract	1512:1524	Mixed Extract	1512:1524	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	2	11	theme	significant	537:547	arg1	differences					549:559	no significant differences	534:559	no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments	534:637	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	7	12	theme	monounsaturated	1258:1272	arg1	SFA					1307:1309	SFA	1307:1309	SFA	1307:1309	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	12	theme	monounsaturated	1258:1272	arg1	acids					1300:1304	saturated, monounsaturated and polyunsaturated fatty acids	1247:1304	saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1247:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	12	theme	monounsaturated	1258:1272	arg1	MUFA					1312:1315	MUFA	1312:1315	MUFA	1312:1315	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	12	theme	monounsaturated	1258:1272	arg1	PUFA					1322:1325	PUFA	1322:1325	PUFA	1322:1325	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	3	13	theme	thiobarbituric	698:711	arg1	TBARS					739:743	TBARS	739:743	TBARS	739:743	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	3	13	theme	thiobarbituric	698:711	arg1	substances					727:736	thiobarbituric acid reactive substances	698:736	thiobarbituric acid reactive substances (TBARS)	698:744	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	1	14	theme	physicochemical	330:344	arg1	characteristics					363:377	the physicochemical, microbiological characteristics	326:377	characteristics	363:377	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	6	15	theme	Chitosan	1099:1106	arg1	treatment					1108:1116	1% Chitosan treatment	1096:1116	1% Chitosan treatment	1096:1116	However, redness values of 1% Chitosan treatment was lower than those obtained from Control sausages and a significant increase was observed between days 30-45.					
31000305	7	16	theme	saturated	1247:1255	arg1	SFA					1307:1309	SFA	1307:1309	SFA	1307:1309	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	16	theme	saturated	1247:1255	arg1	acids					1300:1304	saturated, monounsaturated and polyunsaturated fatty acids	1247:1304	saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1247:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	16	theme	saturated	1247:1255	arg1	MUFA					1312:1315	MUFA	1312:1315	MUFA	1312:1315	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	16	theme	saturated	1247:1255	arg1	PUFA					1322:1325	PUFA	1322:1325	PUFA	1322:1325	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	1	17	theme	microbiological	347:361	arg1	characteristics					363:377	the physicochemical, microbiological characteristics	326:377	characteristics	363:377	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	18	from	ε-polylysine	178:189	arg1	combination					235:245	combination	235:245	combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts)	235:321	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	19	theme	nisin	153:157	arg1	effects					142:148	The effects	138:148	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages	138:470	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	3	20	theme	TVN	775:777	arg1	values					780:785	total volatile nitrogen (TVN) values	750:785	total volatile nitrogen (TVN) values	750:785	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	6	21	theme	1	1096:1096	arg1	%					1097:1097	%	1097:1097	%	1097:1097	However, redness values of 1% Chitosan treatment was lower than those obtained from Control sausages and a significant increase was observed between days 30-45.					
31000305	9	22	theme	promising	1663:1671	arg1	combinations					1627:1638	these combinations	1621:1638	these combinations	1621:1638	Therefore, these combinations could be considered as promising nitrite replacer in frankfurter-type sausages.					
31000305	9	22	theme	promising	1663:1671	arg1	replacer					1681:1688	promising nitrite replacer	1663:1688	promising nitrite replacer in frankfurter-type sausages	1663:1717	Therefore, these combinations could be considered as promising nitrite replacer in frankfurter-type sausages.					
31000305	9	23	theme	nitrite	1673:1679	arg1	combinations					1627:1638	these combinations	1621:1638	these combinations	1621:1638	Therefore, these combinations could be considered as promising nitrite replacer in frankfurter-type sausages.					
31000305	9	23	theme	nitrite	1673:1679	arg1	replacer					1681:1688	promising nitrite replacer	1663:1688	promising nitrite replacer in frankfurter-type sausages	1663:1717	Therefore, these combinations could be considered as promising nitrite replacer in frankfurter-type sausages.					
31000305	3	24	theme	nitrogen	765:772	arg1	values					780:785	total volatile nitrogen (TVN) values	750:785	total volatile nitrogen (TVN) values	750:785	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	3	25	theme	total	750:754	arg1	values					780:785	total volatile nitrogen (TVN) values	750:785	total volatile nitrogen (TVN) values	750:785	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	0	26	theme	nitrite-free	99:110	arg1	sausage					129:135	nitrite-free frankfurter-type sausage	99:135	nitrite-free frankfurter-type sausage	99:135	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.					
31000305	5	27	theme	1	991:991	arg1	%					992:992	%	992:992	%	992:992	1% Chitosan preserved the luminosity of sausages during refrigerated storage.					
31000305	3	28	theme	storage	837:843	arg1	45 days					813:819	45 days	813:819	45 days of refrigerated storage	813:843	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	4	29	theme	%	884:884	arg1	Chitosan					886:893	1% Chitosan	883:893	1% Chitosan	883:893	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	1	30	theme	storage	504:510	arg1	45 days					493:499	45 days	493:499	45 days of storage	493:510	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	0	31	theme	sausage	129:135	arg1	storage					88:94	refrigerated storage	75:94	refrigerated storage of nitrite-free frankfurter-type sausage	75:135	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.					
31000305	7	32	theme	Total	1230:1234	arg1	SFA					1307:1309	SFA	1307:1309	SFA	1307:1309	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	32	theme	Total	1230:1234	arg1	amounts					1236:1242	Total amounts	1230:1242	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1230:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	32	theme	Total	1230:1234	arg1	acids					1300:1304	saturated, monounsaturated and polyunsaturated fatty acids	1247:1304	saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1247:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	32	theme	Total	1230:1234	arg1	MUFA					1312:1315	MUFA	1312:1315	MUFA	1312:1315	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	32	theme	Total	1230:1234	arg1	PUFA					1322:1325	PUFA	1322:1325	PUFA	1322:1325	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	4	33	theme	viable	946:951	arg1	molds					977:981	molds	977:981	molds	977:981	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	4	33	theme	viable	946:951	arg1	TVC					960:962	TVC	960:962	TVC	960:962	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	4	33	theme	viable	946:951	arg1	yeasts					966:971	yeasts	966:971	yeasts	966:971	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	4	33	theme	viable	946:951	arg1	count					953:957	total viable count	940:957	total viable count (TVC)	940:963	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	0	34	theme	Combined	0:7	arg1	effect					9:14	Combined effect	0:14	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.	0:136	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.					
31000305	6	35	theme	Control	1153:1159	arg1	sausages					1161:1168	Control sausages	1153:1168	Control sausages	1153:1168	However, redness values of 1% Chitosan treatment was lower than those obtained from Control sausages and a significant increase was observed between days 30-45.					
31000305	2	36	from	differences	549:559	arg1	moisture					564:571	moisture	564:571	moisture	564:571	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	2	36	from	differences	549:559	arg1	ash					588:590	ash	588:590	ash	588:590	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	2	36	from	differences	549:559	arg1	salt					593:596	salt	593:596	salt	593:596	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	2	36	from	differences	549:559	arg1	fat					574:576	fat	574:576	fat	574:576	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	2	36	from	differences	549:559	arg1	protein					579:585	protein	579:585	protein	579:585	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	2	36	from	differences	549:559	arg1	activity					608:615	water activity	602:615	water activity (aw)	602:620	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	2	36	from	differences	549:559	arg1	aw					618:619	aw	618:619	aw	618:619	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	2	37	theme	water	602:606	arg1	activity					608:615	water activity	602:615	water activity (aw)	602:620	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	2	37	theme	water	602:606	arg1	aw					618:619	aw	618:619	aw	618:619	The results revealed no significant differences on moisture, fat, protein, ash, salt and water activity (aw) among treatments.					
31000305	0	38	theme	antioxidants	27:38	arg1	effect					9:14	Combined effect	0:14	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.	0:136	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.					
31000305	7	39	theme	natural	1407:1413	arg1	antioxidants					1415:1426	natural antioxidants	1407:1426	natural antioxidants	1407:1426	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	1	40	dep	leaves	306:311	arg1	tea					275:277	green tea	269:277	green tea	269:277	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	41	theme	nitrite-free	433:444	arg1	sausages					463:470	the nitrite-free frankfurter-type sausages	429:470	the nitrite-free frankfurter-type sausages	429:470	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	0	42	theme	antimicrobial	44:56	arg1	compounds					58:66	antimicrobial compounds	44:66	antimicrobial compounds	44:66	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.					
31000305	9	43	from	replacer	1681:1688	arg1	sausages					1710:1717	frankfurter-type sausages	1693:1717	frankfurter-type sausages	1693:1717	Therefore, these combinations could be considered as promising nitrite replacer in frankfurter-type sausages.					
31000305	8	44	theme	ε-Polylysine	1478:1489	arg1	Combinations					1457:1468	Combinations	1457:1468	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract	1457:1524	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	1	45	theme	sausages	463:470	arg1	composition					391:401	fatty acid composition	380:401	fatty acid composition	380:401	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	45	theme	sausages	463:470	arg1	attributes					415:424	sensory attributes	407:424	sensory attributes	407:424	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	45	theme	sausages	463:470	arg1	characteristics					363:377	the physicochemical, microbiological characteristics	326:377	characteristics	363:377	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	8	46	theme	1	1495:1495	arg1	%					1496:1496	%	1496:1496	%	1496:1496	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	1	47	theme	chitosan	210:217	arg1	effects					142:148	The effects	138:148	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages	138:470	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	5	48	theme	refrigerated	1047:1058	arg1	storage					1060:1066	refrigerated storage	1047:1066	refrigerated storage	1047:1066	1% Chitosan preserved the luminosity of sausages during refrigerated storage.					
31000305	6	49	theme	significant	1176:1186	arg1	increase					1188:1195	a significant increase	1174:1195	a significant increase	1174:1195	However, redness values of 1% Chitosan treatment was lower than those obtained from Control sausages and a significant increase was observed between days 30-45.					
31000305	8	50	theme	Mixed	1512:1516	arg1	Extract					1518:1524	Mixed Extract	1512:1524	Mixed Extract	1512:1524	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	1	51	theme	fatty	380:384	arg1	composition					391:401	fatty acid composition	380:401	fatty acid composition	380:401	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	8	52	theme	0.2	1473:1475	arg1	%					1476:1476	%	1476:1476	%	1476:1476	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	8	53	theme	nitrite-free	1570:1581	arg1	sausages					1600:1607	nitrite-free frankfurter-type sausages	1570:1607	nitrite-free frankfurter-type sausages	1570:1607	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	7	54	theme	frankfurter-type	1331:1346	arg1	sausages					1348:1355	frankfurter-type sausages	1331:1355	frankfurter-type sausages	1331:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	6	55	theme	redness	1078:1084	arg1	values					1086:1091	redness values	1078:1091	redness values of 1% Chitosan treatment	1078:1116	However, redness values of 1% Chitosan treatment was lower than those obtained from Control sausages and a significant increase was observed between days 30-45.					
31000305	3	56	theme	Extract	660:666	arg1	sausages					668:675	1% Chitosan + Mixed Extract sausages	640:675	1% Chitosan + Mixed Extract sausages	640:675	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	1	57	from	combination	235:245	arg1	effects					142:148	The effects	138:148	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages	138:470	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	8	58	theme	sausages	1600:1607	arg1	life					1562:1565	the shelf life	1552:1565	the shelf life of nitrite-free frankfurter-type sausages	1552:1607	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	1	59	with	combination	235:245	arg1	Extract					260:266	a Mixed Extract	252:266	a Mixed Extract (green tea, stinging nettle and olive leaves extracts)	252:321	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	3	60	theme	%	641:641	arg1	sausages					668:675	1% Chitosan + Mixed Extract sausages	640:675	1% Chitosan + Mixed Extract sausages	640:675	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	1	61	theme	sensory	407:413	arg1	attributes					415:424	sensory attributes	407:424	sensory attributes	407:424	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	62	theme	ε-polylysine	178:189	arg1	effects					142:148	The effects	138:148	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages	138:470	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	3	63	theme	lowest	691:696	arg1	TBARS					739:743	TBARS	739:743	TBARS	739:743	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	3	63	theme	lowest	691:696	arg1	substances					727:736	thiobarbituric acid reactive substances	698:736	thiobarbituric acid reactive substances (TBARS)	698:744	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	6	64	dep	days	1218:1221	arg1	30-45					1223:1227	30-45	1223:1227	30-45	1223:1227	However, redness values of 1% Chitosan treatment was lower than those obtained from Control sausages and a significant increase was observed between days 30-45.					
31000305	4	65	theme	0.2	862:864	arg1	%					865:865	%	865:865	%	865:865	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	7	66	theme	fatty	1294:1298	arg1	SFA					1307:1309	SFA	1307:1309	SFA	1307:1309	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	66	theme	fatty	1294:1298	arg1	acids					1300:1304	saturated, monounsaturated and polyunsaturated fatty acids	1247:1304	saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1247:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	66	theme	fatty	1294:1298	arg1	MUFA					1312:1315	MUFA	1312:1315	MUFA	1312:1315	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	66	theme	fatty	1294:1298	arg1	PUFA					1322:1325	PUFA	1322:1325	PUFA	1322:1325	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	1	67	from	chitosan	210:217	arg1	combination					235:245	combination	235:245	combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts)	235:321	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	5	68	theme	sausages	1031:1038	arg1	luminosity					1017:1026	the luminosity	1013:1026	the luminosity of sausages	1013:1038	1% Chitosan preserved the luminosity of sausages during refrigerated storage.					
31000305	6	69	theme	treatment	1108:1116	arg1	values					1086:1091	redness values	1078:1091	redness values of 1% Chitosan treatment	1078:1116	However, redness values of 1% Chitosan treatment was lower than those obtained from Control sausages and a significant increase was observed between days 30-45.					
31000305	3	70	theme	acid	713:716	arg1	TBARS					739:743	TBARS	739:743	TBARS	739:743	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	3	70	theme	acid	713:716	arg1	substances					727:736	thiobarbituric acid reactive substances	698:736	thiobarbituric acid reactive substances (TBARS)	698:744	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	6	71	theme	%	1097:1097	arg1	treatment					1108:1116	1% Chitosan treatment	1096:1116	1% Chitosan treatment	1096:1116	However, redness values of 1% Chitosan treatment was lower than those obtained from Control sausages and a significant increase was observed between days 30-45.					
31000305	0	72	theme	refrigerated	75:86	arg1	storage					88:94	refrigerated storage	75:94	refrigerated storage of nitrite-free frankfurter-type sausage	75:135	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.					
31000305	7	73	dep	acids	1300:1304	arg1	SFA					1307:1309	SFA	1307:1309	SFA	1307:1309	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	73	dep	acids	1300:1304	arg1	acids					1300:1304	saturated, monounsaturated and polyunsaturated fatty acids	1247:1304	saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages	1247:1355	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	73	dep	acids	1300:1304	arg1	MUFA					1312:1315	MUFA	1312:1315	MUFA	1312:1315	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	7	73	dep	acids	1300:1304	arg1	PUFA					1322:1325	PUFA	1322:1325	PUFA	1322:1325	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	4	74	theme	Chitosan	886:893	arg1	Combinations					846:857	Combinations	846:857	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract	846:912	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	4	75	theme	Mixed	900:904	arg1	Extract					906:912	Mixed Extract	900:912	Mixed Extract	900:912	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	0	76	theme	frankfurter-type	112:127	arg1	sausage					129:135	nitrite-free frankfurter-type sausage	99:135	nitrite-free frankfurter-type sausage	99:135	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.					
31000305	1	77	theme	stinging	280:287	arg1	nettle					289:294	stinging nettle	280:294	stinging nettle	280:294	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	7	78	theme	compounds	1446:1454	arg1	combination					1392:1402	the combination	1388:1402	the combination of natural antioxidants and antimicrobial compounds	1388:1454	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	4	79	theme	ε-Polylysine	867:878	arg1	Combinations					846:857	Combinations	846:857	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract	846:912	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	1	80	dep	Extract	260:266	arg1	leaves					306:311	leaves	306:311	leaves extracts	306:320	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	81	dep	nisin	153:157	arg1	200					160:162	200	160:162	200	160:162	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	81	dep	nisin	153:157	arg1	400 ppm					168:174	400 ppm	168:174	400 ppm	168:174	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	3	82	theme	volatile	756:763	arg1	values					780:785	total volatile nitrogen (TVN) values	750:785	total volatile nitrogen (TVN) values	750:785	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	5	83	theme	%	992:992	arg1	Chitosan					994:1001	1% Chitosan	991:1001	1% Chitosan	991:1001	1% Chitosan preserved the luminosity of sausages during refrigerated storage.					
31000305	4	84	theme	1	883:883	arg1	%					884:884	%	884:884	%	884:884	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	9	85	theme	frankfurter-type	1693:1708	arg1	sausages					1710:1717	frankfurter-type sausages	1693:1717	frankfurter-type sausages	1693:1717	Therefore, these combinations could be considered as promising nitrite replacer in frankfurter-type sausages.					
31000305	3	86	dep	substances	727:736	arg1	the					687:689	the	687:689	the	687:689	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	1	87	from	nisin	153:157	arg1	combination					235:245	combination	235:245	combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts)	235:321	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	3	88	theme	refrigerated	824:835	arg1	storage					837:843	refrigerated storage	824:843	refrigerated storage	824:843	1% Chitosan + Mixed Extract sausages displayed the lowest thiobarbituric acid reactive substances (TBARS) and total volatile nitrogen (TVN) values compared to Control after 45 days of refrigerated storage.					
31000305	4	89	theme	total	940:944	arg1	molds					977:981	molds	977:981	molds	977:981	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	4	89	theme	total	940:944	arg1	TVC					960:962	TVC	960:962	TVC	960:962	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	4	89	theme	total	940:944	arg1	yeasts					966:971	yeasts	966:971	yeasts	966:971	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	4	89	theme	total	940:944	arg1	count					953:957	total viable count	940:957	total viable count (TVC)	940:963	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	0	90	theme	natural	19:25	arg1	antioxidants					27:38	natural antioxidants	19:38	natural antioxidants	19:38	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.					
31000305	1	91	from	effects	142:148	arg1	combination					235:245	combination	235:245	combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts)	235:321	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	91	from	effects	142:148	arg1	composition					391:401	fatty acid composition	380:401	fatty acid composition	380:401	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	91	from	effects	142:148	arg1	attributes					415:424	sensory attributes	407:424	sensory attributes	407:424	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	1	91	from	effects	142:148	arg1	characteristics					363:377	the physicochemical, microbiological characteristics	326:377	characteristics	363:377	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	4	92	theme	count	953:957	arg1	growth					983:988	total viable count (TVC), yeasts and molds growth	940:988	total viable count (TVC), yeasts and molds growth	940:988	Combinations of 0.2% ε-Polylysine or 1% Chitosan with Mixed Extract were effective to inhibit total viable count (TVC), yeasts and molds growth.					
31000305	7	93	theme	antimicrobial	1432:1444	arg1	compounds					1446:1454	antimicrobial compounds	1432:1454	antimicrobial compounds	1432:1454	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	8	94	theme	%	1476:1476	arg1	ε-Polylysine					1478:1489	0.2% ε-Polylysine	1473:1489	0.2% ε-Polylysine	1473:1489	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	0	95	theme	compounds	58:66	arg1	effect					9:14	Combined effect	0:14	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.	0:136	Combined effect of natural antioxidants and antimicrobial compounds during refrigerated storage of nitrite-free frankfurter-type sausage.					
31000305	1	96	theme	Mixed	254:258	arg1	Extract					260:266	a Mixed Extract	252:266	a Mixed Extract (green tea, stinging nettle and olive leaves extracts)	252:321	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	7	97	theme	antioxidants	1415:1426	arg1	combination					1392:1402	the combination	1388:1402	the combination of natural antioxidants and antimicrobial compounds	1388:1454	Total amounts of saturated, monounsaturated and polyunsaturated fatty acids (SFA, MUFA, and PUFA) of frankfurter-type sausages were not largely influenced by the combination of natural antioxidants and antimicrobial compounds.					
31000305	1	98	theme	frankfurter-type	446:461	arg1	sausages					463:470	the nitrite-free frankfurter-type sausages	429:470	the nitrite-free frankfurter-type sausages	429:470	The effects of nisin (200 and 400 ppm), ε-polylysine (0.1 and 0.2%) and chitosan (0.5 and 1%) in combination with a Mixed Extract (green tea, stinging nettle and olive leaves extracts) on the physicochemical, microbiological characteristics, fatty acid composition and sensory attributes of the nitrite-free frankfurter-type sausages were assessed during 45 days of storage.					
31000305	8	99	theme	%	1496:1496	arg1	Chitosan					1498:1505	1% Chitosan	1495:1505	1% Chitosan	1495:1505	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	8	100	theme	shelf	1556:1560	arg1	life					1562:1565	the shelf life	1552:1565	the shelf life of nitrite-free frankfurter-type sausages	1552:1607	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
31000305	8	101	theme	Chitosan	1498:1505	arg1	Combinations					1457:1468	Combinations	1457:1468	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract	1457:1524	Combinations of 0.2% ε-Polylysine and 1% Chitosan with Mixed Extract were effective to improve the shelf life of nitrite-free frankfurter-type sausages.					
29803848	11	0	theme	microbiota	2026:2035	arg1	structure					2037:2045	the nasal microbiota structure	2016:2045	the nasal microbiota structure	2016:2045	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	3	1	theme	growing	758:764	arg1	pigs					766:769	growing pigs	758:769	growing pigs	758:769	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	1	2	theme	microbial	284:292	arg1	communities					294:304	the beneficial microbial communities	269:304	the beneficial microbial communities	269:304	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	9	3	theme	groups	1635:1640	arg1	compositions					1615:1626	the nasal microbial compositions	1595:1626	the nasal microbial compositions of the groups receiving CCFA and OTC	1595:1663	By day 14, the nasal microbial compositions of the groups receiving CCFA and OTC had returned to a distribution that closely resembled that observed on day 0.					
29803848	2	4	from	effects	361:367	arg1	biodiversity					438:449	swine gastrointestinal microbiota biodiversity	404:449	swine gastrointestinal microbiota biodiversity	404:449	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	6	5	theme	nasal	1237:1241	arg1	microbiota					1243:1252	The nasal microbiota	1233:1252	The nasal microbiota across all the samples	1233:1275	The nasal microbiota across all the samples were dominated by Firmicutes, proteobacteria and Bacteroidetes.					
29803848	2	6	theme	increasing	316:325	arg1	number					327:332	an increasing number	313:332	an increasing number of studies	313:343	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	4	7	theme	Crystalline	913:923	arg1	CCFA					936:939	CCFA	936:939	CCFA	936:939	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	7	theme	Crystalline	913:923	arg1	acid					930:933	Ceftiofur Crystalline free acid	903:933	Ceftiofur Crystalline free acid (CCFA)	903:940	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	7	theme	Crystalline	913:923	arg1	antimicrobials					887:900	the antimicrobials	883:900	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG)	883:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	11	8	theme	antibiotics	1918:1928	arg1	variable					1963:1970	variable	1963:1970	variable	1963:1970	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	11	8	theme	antibiotics	1918:1928	arg1	impact					1897:1902	the impact	1893:1902	the impact of parenteral antibiotics on the swine nasal microbiota	1893:1958	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	3	9	theme	short-term	622:631	arg1	impact					633:638	the short-term impact	618:638	the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs	618:769	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	11	10	theme	nasal	1943:1947	arg1	microbiota					1949:1958	the swine nasal microbiota	1933:1958	the swine nasal microbiota	1933:1958	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	4	11	dep	antimicrobials	887:900	arg1	G					1042:1042	Procaine Penicillin G	1022:1042	Procaine Penicillin G (PPG)	1022:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	Tulathromycin					974:986	Tulathromycin	974:986	Tulathromycin (TUL)	974:992	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	OTC					1012:1014	OTC	1012:1014	OTC	1012:1014	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	acid					930:933	Ceftiofur Crystalline free acid	903:933	Ceftiofur Crystalline free acid (CCFA)	903:940	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	hydrochloride					953:965	Ceftiofur hydrochloride	943:965	Ceftiofur hydrochloride (CHC)	943:971	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	PPG					1045:1047	PPG	1045:1047	PPG	1045:1047	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	TUL					989:991	TUL	989:991	TUL	989:991	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	CHC					968:970	CHC	968:970	CHC	968:970	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	antimicrobials					887:900	the antimicrobials	883:900	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG)	883:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	CCFA					936:939	CCFA	936:939	CCFA	936:939	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	11	dep	antimicrobials	887:900	arg1	Oxytetracycline					995:1009	Oxytetracycline	995:1009	Oxytetracycline (OTC)	995:1015	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	8	12	theme	Linear	1428:1433	arg1	analysis					1448:1455	Linear discriminant analysis	1428:1455	Linear discriminant analysis	1428:1455	Linear discriminant analysis, showed a pronounced, antimicrobial-dependent microbial shift in the composition of nasal microbiota and over time from day 0.					
29803848	7	13	theme	predominant	1352:1362	arg1	Moraxella					1386:1394	Moraxella	1386:1394	Moraxella	1386:1394	While, the predominant bacterial genera were Moraxella, Clostridium and Streptococcus.					
29803848	7	13	theme	predominant	1352:1362	arg1	genera					1374:1379	the predominant bacterial genera	1348:1379	the predominant bacterial genera	1348:1379	While, the predominant bacterial genera were Moraxella, Clostridium and Streptococcus.					
29803848	5	14	theme	deep	1086:1089	arg1	swabs					1097:1101	Individual deep nasal swabs	1075:1101	Individual deep nasal swabs	1075:1101	Individual deep nasal swabs were collected immediately before antimicrobial administration (control = day 0), and again on days 1, 3, 7, and 14 after dosing.					
29803848	3	15	theme	parenteral	653:662	arg1	antibiotics					664:674	different parenteral antibiotics	643:674	different parenteral antibiotics administration	643:689	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	1	16	theme	bacteria	243:250	arg1	dysbiosis					256:264	dysbiosis	256:264	dysbiosis of the beneficial microbial communities	256:304	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	1	16	theme	bacteria	243:250	arg1	increase					207:214	the increase	203:214	the increase of antimicrobial-resistant bacteria	203:250	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	4	17	theme	treatment	791:799	arg1	each					809:812	each	809:812	each	809:812	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	17	theme	treatment	791:799	arg1	groups					801:806	Five antimicrobial treatment groups	772:806	Five antimicrobial treatment groups	772:806	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	11	18	theme	considerable	1982:1993	arg1	impact					1995:2000	a considerable impact	1980:2000	a considerable impact	1980:2000	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	8	19	theme	pronounced	1467:1476	arg1	shift					1513:1517	a pronounced, antimicrobial-dependent microbial shift	1465:1517	shift	1513:1517	Linear discriminant analysis, showed a pronounced, antimicrobial-dependent microbial shift in the composition of nasal microbiota and over time from day 0.					
29803848	3	20	theme	administration	676:689	arg1	impact					633:638	the short-term impact	618:638	the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs	618:769	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	8	21	theme	antimicrobial-dependent	1479:1501	arg1	shift					1513:1517	a pronounced, antimicrobial-dependent microbial shift	1465:1517	shift	1513:1517	Linear discriminant analysis, showed a pronounced, antimicrobial-dependent microbial shift in the composition of nasal microbiota and over time from day 0.					
29803848	0	22	theme	parenteral	62:71	arg1	administration					87:100	parenteral antimicrobial administration	62:100	parenteral antimicrobial administration	62:100	Microbial shifts in the swine nasal microbiota in response to parenteral antimicrobial administration.					
29803848	4	23	theme	eight-week	834:843	arg1	four					828:831	four	828:831	four	828:831	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	23	theme	eight-week	834:843	arg1	piglets					849:855	eight-week old piglets	834:855	eight-week old piglets	834:855	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	24	dep	dose	1059:1062	arg1	label					1053:1057	label	1053:1057	label	1053:1057	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	25	theme	Procaine	1022:1029	arg1	PPG					1045:1047	PPG	1045:1047	PPG	1045:1047	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	25	theme	Procaine	1022:1029	arg1	G					1042:1042	Procaine Penicillin G	1022:1042	Procaine Penicillin G (PPG)	1022:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	25	theme	Procaine	1022:1029	arg1	antimicrobials					887:900	the antimicrobials	883:900	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG)	883:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	12	26	theme	swine	2133:2137	arg1	health					2139:2144	swine health	2133:2144	swine health	2133:2144	Our results will aid in developing alternative strategies for antibiotics to improve swine health and consequently production.					
29803848	0	27	theme	Microbial	0:8	arg1	shifts					10:15	Microbial shifts	0:15	Microbial shifts in the swine nasal microbiota in response to parenteral antimicrobial administration.	0:101	Microbial shifts in the swine nasal microbiota in response to parenteral antimicrobial administration.					
29803848	1	28	from	administration	118:131	arg1	production					160:169	swine production	154:169	swine production	154:169	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	8	29	theme	microbiota	1547:1556	arg1	composition					1526:1536	the composition	1522:1536	the composition of nasal microbiota	1522:1556	Linear discriminant analysis, showed a pronounced, antimicrobial-dependent microbial shift in the composition of nasal microbiota and over time from day 0.					
29803848	1	30	theme	continuous	107:116	arg1	administration					118:131	The continuous administration	103:131	The continuous administration of antimicrobials in swine production	103:169	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	2	31	theme	communities	528:538	arg1	diversity					499:507	diversity	499:507	diversity	499:507	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	2	31	theme	communities	528:538	arg1	composition					483:493	composition	483:493	composition	483:493	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	0	32	theme	swine	24:28	arg1	microbiota					36:45	the swine nasal microbiota	20:45	the swine nasal microbiota	20:45	Microbial shifts in the swine nasal microbiota in response to parenteral antimicrobial administration.					
29803848	2	33	theme	nasal	512:516	arg1	communities					528:538	nasal microbial communities	512:538	nasal microbial communities	512:538	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	8	34	from	shift	1513:1517	arg1	composition					1526:1536	the composition	1522:1536	the composition of nasal microbiota	1522:1556	Linear discriminant analysis, showed a pronounced, antimicrobial-dependent microbial shift in the composition of nasal microbiota and over time from day 0.					
29803848	2	35	theme	administration	386:399	arg1	effects					361:367	the effects	357:367	the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity	357:449	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	2	36	from	impact	456:461	arg1	diversity					499:507	diversity	499:507	diversity	499:507	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	2	36	from	impact	456:461	arg1	composition					483:493	composition	483:493	composition	483:493	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	10	37	theme	day	1826:1828	arg1	composition					1832:1842	the day 0 composition	1822:1842	the day 0 composition by day 14	1822:1852	In contrast, pigs that received CHC, TUL and PPG appeared to deviate away from the day 0 composition by day 14.					
29803848	3	38	dep	composition	698:708	arg1	the					694:696	the	694:696	the	694:696	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	9	39	theme	nasal	1599:1603	arg1	compositions					1615:1626	the nasal microbial compositions	1595:1626	the nasal microbial compositions of the groups receiving CCFA and OTC	1595:1663	By day 14, the nasal microbial compositions of the groups receiving CCFA and OTC had returned to a distribution that closely resembled that observed on day 0.					
29803848	3	40	theme	microbial	733:741	arg1	communities					743:753	nasal microbial communities	727:753	nasal microbial communities	727:753	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	12	41	theme	alternative	2083:2093	arg1	strategies					2095:2104	alternative strategies	2083:2104	alternative strategies for antibiotics	2083:2120	Our results will aid in developing alternative strategies for antibiotics to improve swine health and consequently production.					
29803848	2	42	theme	microbiota	427:436	arg1	biodiversity					438:449	swine gastrointestinal microbiota biodiversity	404:449	swine gastrointestinal microbiota biodiversity	404:449	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	2	43	theme	swine	404:408	arg1	biodiversity					438:449	swine gastrointestinal microbiota biodiversity	404:449	swine gastrointestinal microbiota biodiversity	404:449	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	1	44	theme	beneficial	273:282	arg1	communities					294:304	the beneficial microbial communities	269:304	the beneficial microbial communities	269:304	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	3	45	from	composition	698:708	arg1	pigs					766:769	growing pigs	758:769	growing pigs	758:769	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	3	46	theme	study	592:596	arg1	objective					574:582	The objective	570:582	The objective of this study	570:596	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	1	47	theme	communities	294:304	arg1	dysbiosis					256:264	dysbiosis	256:264	dysbiosis of the beneficial microbial communities	256:304	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	1	47	theme	communities	294:304	arg1	increase					207:214	the increase	203:214	the increase of antimicrobial-resistant bacteria	203:250	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	11	48	theme	nasal	2020:2024	arg1	structure					2037:2045	the nasal microbiota structure	2016:2045	the nasal microbiota structure	2016:2045	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	3	49	from	diversity	714:722	arg1	pigs					766:769	growing pigs	758:769	growing pigs	758:769	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	11	50	theme	parenteral	1907:1916	arg1	antibiotics					1918:1928	parenteral antibiotics	1907:1928	parenteral antibiotics	1907:1928	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	4	51	theme	Ceftiofur	903:911	arg1	CCFA					936:939	CCFA	936:939	CCFA	936:939	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	51	theme	Ceftiofur	903:911	arg1	acid					930:933	Ceftiofur Crystalline free acid	903:933	Ceftiofur Crystalline free acid (CCFA)	903:940	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	51	theme	Ceftiofur	903:911	arg1	antimicrobials					887:900	the antimicrobials	883:900	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG)	883:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	11	52	from	impact	1897:1902	arg1	microbiota					1949:1958	the swine nasal microbiota	1933:1958	the swine nasal microbiota	1933:1958	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	8	53	from	day	1577:1579	arg1	shift					1513:1517	a pronounced, antimicrobial-dependent microbial shift	1465:1517	shift	1513:1517	Linear discriminant analysis, showed a pronounced, antimicrobial-dependent microbial shift in the composition of nasal microbiota and over time from day 0.					
29803848	4	54	theme	free	925:928	arg1	CCFA					936:939	CCFA	936:939	CCFA	936:939	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	54	theme	free	925:928	arg1	acid					930:933	Ceftiofur Crystalline free acid	903:933	Ceftiofur Crystalline free acid (CCFA)	903:940	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	54	theme	free	925:928	arg1	antimicrobials					887:900	the antimicrobials	883:900	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG)	883:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	2	55	theme	studies	337:343	arg1	number					327:332	an increasing number	313:332	an increasing number of studies	313:343	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	8	56	theme	discriminant	1435:1446	arg1	analysis					1448:1455	Linear discriminant analysis	1428:1455	Linear discriminant analysis	1428:1455	Linear discriminant analysis, showed a pronounced, antimicrobial-dependent microbial shift in the composition of nasal microbiota and over time from day 0.					
29803848	5	57	theme	Individual	1075:1084	arg1	swabs					1097:1101	Individual deep nasal swabs	1075:1101	Individual deep nasal swabs	1075:1101	Individual deep nasal swabs were collected immediately before antimicrobial administration (control = day 0), and again on days 1, 3, 7, and 14 after dosing.					
29803848	3	58	theme	different	643:651	arg1	antibiotics					664:674	different parenteral antibiotics	643:674	different parenteral antibiotics administration	643:689	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	1	59	theme	antimicrobial-resistant	219:241	arg1	bacteria					243:250	antimicrobial-resistant bacteria	219:250	antimicrobial-resistant bacteria	219:250	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	7	60	theme	bacterial	1364:1372	arg1	Moraxella					1386:1394	Moraxella	1386:1394	Moraxella	1386:1394	While, the predominant bacterial genera were Moraxella, Clostridium and Streptococcus.					
29803848	7	60	theme	bacterial	1364:1372	arg1	genera					1374:1379	the predominant bacterial genera	1348:1379	the predominant bacterial genera	1348:1379	While, the predominant bacterial genera were Moraxella, Clostridium and Streptococcus.					
29803848	5	61	theme	nasal	1091:1095	arg1	swabs					1097:1101	Individual deep nasal swabs	1075:1101	Individual deep nasal swabs	1075:1101	Individual deep nasal swabs were collected immediately before antimicrobial administration (control = day 0), and again on days 1, 3, 7, and 14 after dosing.					
29803848	4	62	theme	Ceftiofur	943:951	arg1	CHC					968:970	CHC	968:970	CHC	968:970	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	62	theme	Ceftiofur	943:951	arg1	antimicrobials					887:900	the antimicrobials	883:900	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG)	883:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	62	theme	Ceftiofur	943:951	arg1	hydrochloride					953:965	Ceftiofur hydrochloride	943:965	Ceftiofur hydrochloride (CHC)	943:971	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	63	theme	antimicrobial	777:789	arg1	each					809:812	each	809:812	each	809:812	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	63	theme	antimicrobial	777:789	arg1	groups					801:806	Five antimicrobial treatment groups	772:806	Five antimicrobial treatment groups	772:806	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	1	64	theme	antimicrobials	136:149	arg1	administration					118:131	The continuous administration	103:131	The continuous administration of antimicrobials in swine production	103:169	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	11	65	theme	swine	1937:1941	arg1	microbiota					1949:1958	the swine nasal microbiota	1933:1958	the swine nasal microbiota	1933:1958	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	3	66	theme	antibiotics	664:674	arg1	administration					676:689	different parenteral antibiotics administration	643:689	different parenteral antibiotics administration	643:689	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	1	67	theme	swine	154:158	arg1	production					160:169	swine production	154:169	swine production	154:169	The continuous administration of antimicrobials in swine production has been widely criticized with the increase of antimicrobial-resistant bacteria and dysbiosis of the beneficial microbial communities.					
29803848	8	68	theme	microbial	1503:1511	arg1	shift					1513:1517	a pronounced, antimicrobial-dependent microbial shift	1465:1517	shift	1513:1517	Linear discriminant analysis, showed a pronounced, antimicrobial-dependent microbial shift in the composition of nasal microbiota and over time from day 0.					
29803848	0	69	theme	antimicrobial	73:85	arg1	administration					87:100	parenteral antimicrobial administration	62:100	parenteral antimicrobial administration	62:100	Microbial shifts in the swine nasal microbiota in response to parenteral antimicrobial administration.					
29803848	5	70	theme	antimicrobial	1137:1149	arg1	control = day					1167:1179	control = day 0	1167:1181	control = day 0	1167:1181	Individual deep nasal swabs were collected immediately before antimicrobial administration (control = day 0), and again on days 1, 3, 7, and 14 after dosing.					
29803848	5	70	theme	antimicrobial	1137:1149	arg1	administration					1151:1164	antimicrobial administration	1137:1164	antimicrobial administration (control = day 0)	1137:1182	Individual deep nasal swabs were collected immediately before antimicrobial administration (control = day 0), and again on days 1, 3, 7, and 14 after dosing.					
29803848	0	71	from	shifts	10:15	arg1	response					50:57	response	50:57	response to parenteral antimicrobial administration	50:100	Microbial shifts in the swine nasal microbiota in response to parenteral antimicrobial administration.					
29803848	0	71	from	shifts	10:15	arg1	microbiota					36:45	the swine nasal microbiota	20:45	the swine nasal microbiota	20:45	Microbial shifts in the swine nasal microbiota in response to parenteral antimicrobial administration.					
29803848	11	72	contain	has	1976:1978	arg2	impact					1995:2000	a considerable impact	1980:2000	a considerable impact	1980:2000	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	11	72	contain	has	1976:1978	arg1	impact					1897:1902	the impact	1893:1902	the impact of parenteral antibiotics on the swine nasal microbiota	1893:1958	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	11	72	contain	has	1976:1978	arg1	variable					1963:1970	variable	1963:1970	variable	1963:1970	Based on our results, it appears that the impact of parenteral antibiotics on the swine nasal microbiota is variable and has a considerable impact in modulating the nasal microbiota structure.					
29803848	2	73	dep	composition	483:493	arg1	the					479:481	the	479:481	the	479:481	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	8	74	theme	nasal	1541:1545	arg1	microbiota					1547:1556	nasal microbiota	1541:1556	nasal microbiota	1541:1556	Linear discriminant analysis, showed a pronounced, antimicrobial-dependent microbial shift in the composition of nasal microbiota and over time from day 0.					
29803848	4	75	theme	old	845:847	arg1	four					828:831	four	828:831	four	828:831	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	75	theme	old	845:847	arg1	piglets					849:855	eight-week old piglets	834:855	eight-week old piglets	834:855	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	3	76	from	impact	633:638	arg1	diversity					714:722	diversity	714:722	diversity	714:722	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	3	76	from	impact	633:638	arg1	composition					698:708	composition	698:708	composition	698:708	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	2	77	theme	use	472:474	arg1	impact					456:461	the impact	452:461	the impact of their use on the composition and diversity of nasal microbial communities	452:538	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	0	78	theme	nasal	30:34	arg1	microbiota					36:45	the swine nasal microbiota	20:45	the swine nasal microbiota	20:45	Microbial shifts in the swine nasal microbiota in response to parenteral antimicrobial administration.					
29803848	2	79	theme	microbial	518:526	arg1	communities					528:538	nasal microbial communities	512:538	nasal microbial communities	512:538	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	4	80	theme	Penicillin	1031:1040	arg1	PPG					1045:1047	PPG	1045:1047	PPG	1045:1047	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	80	theme	Penicillin	1031:1040	arg1	G					1042:1042	Procaine Penicillin G	1022:1042	Procaine Penicillin G (PPG)	1022:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	80	theme	Penicillin	1031:1040	arg1	antimicrobials					887:900	the antimicrobials	883:900	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG)	883:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	3	81	theme	communities	743:753	arg1	diversity					714:722	diversity	714:722	diversity	714:722	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	3	81	theme	communities	743:753	arg1	composition					698:708	composition	698:708	composition	698:708	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	4	82	theme	antimicrobials	887:900	arg1	one					876:878	one	876:878	one	876:878	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	82	theme	antimicrobials	887:900	arg1	Tulathromycin					974:986	Tulathromycin	974:986	Tulathromycin (TUL)	974:992	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	82	theme	antimicrobials	887:900	arg1	acid					930:933	Ceftiofur Crystalline free acid	903:933	Ceftiofur Crystalline free acid (CCFA)	903:940	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	82	theme	antimicrobials	887:900	arg1	hydrochloride					953:965	Ceftiofur hydrochloride	943:965	Ceftiofur hydrochloride (CHC)	943:971	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	82	theme	antimicrobials	887:900	arg1	G					1042:1042	Procaine Penicillin G	1022:1042	Procaine Penicillin G (PPG)	1022:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	82	theme	antimicrobials	887:900	arg1	antimicrobials					887:900	the antimicrobials	883:900	the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG)	883:1048	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	4	82	theme	antimicrobials	887:900	arg1	Oxytetracycline					995:1009	Oxytetracycline	995:1009	Oxytetracycline (OTC)	995:1015	Five antimicrobial treatment groups, each consisting of four, eight-week old piglets, were administered one of the antimicrobials; Ceftiofur Crystalline free acid (CCFA), Ceftiofur hydrochloride (CHC), Tulathromycin (TUL), Oxytetracycline (OTC), and Procaine Penicillin G (PPG) at label dose and route.					
29803848	2	83	theme	antimicrobial	372:384	arg1	administration					386:399	antimicrobial administration	372:399	antimicrobial administration	372:399	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
29803848	3	84	theme	nasal	727:731	arg1	communities					743:753	nasal microbial communities	727:753	nasal microbial communities	727:753	The objective of this study was to characterize the short-term impact of different parenteral antibiotics administration on the composition and diversity of nasal microbial communities in growing pigs.					
29803848	9	85	theme	microbial	1605:1613	arg1	compositions					1615:1626	the nasal microbial compositions	1595:1626	the nasal microbial compositions of the groups receiving CCFA and OTC	1595:1663	By day 14, the nasal microbial compositions of the groups receiving CCFA and OTC had returned to a distribution that closely resembled that observed on day 0.					
29803848	2	86	theme	gastrointestinal	410:425	arg1	biodiversity					438:449	swine gastrointestinal microbiota biodiversity	404:449	swine gastrointestinal microbiota biodiversity	404:449	While an increasing number of studies investigate the effects of antimicrobial administration on swine gastrointestinal microbiota biodiversity, the impact of their use on the composition and diversity of nasal microbial communities has not been widely explored.					
30328901	3	0	with	DMM	450:452	arg1	DMM+s					588:592	DMM+s	588:592	DMM+s	588:592	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	0	with	DMM	450:452	arg1	DMM+s					474:478	DMM+s	474:478	DMM+s	474:478	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	0	with	DMM	450:452	arg1	DMM					562:564	DMM	562:564	DMM	562:564	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	0	with	DMM	450:452	arg1	DMM+s					538:542	DMM+s	538:542	DMM+s	538:542	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	0	with	DMM	450:452	arg1	%					460:460	1%	459:460	1% of sucrose (DMM+s) (positive control)	459:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	0	with	DMM	450:452	arg1	DMM					517:519	DMM	517:519	DMM	517:519	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	0	with	DMM	450:452	arg1	sucrose					465:471	sucrose	465:471	sucrose (DMM+s) (positive control)	465:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	0	with	DMM	450:452	arg1	control					491:497	positive control	482:497	positive control	482:497	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	4	1	theme	supernatant	714:724	arg1	pH					708:709	pH	708:709	pH of supernatant	708:724	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	4	1	theme	supernatant	714:724	arg1	composition					679:689	microbiological composition	663:689	microbiological composition of biofilms	663:701	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	4	1	theme	supernatant	714:724	arg1	variables					621:629	the outcome variables surface hardness change (%SHC)	609:660	the outcome variables surface hardness change (%SHC)	609:660	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	10	2	theme	aciduric	1338:1345	arg1	microorganisms					1347:1360	total aciduric microorganisms	1332:1360	total aciduric microorganisms	1332:1360	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	11	3	dep	milks	1472:1476	arg1	both					1453:1456	both	1453:1456	both	1453:1456	Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.					
30328901	11	3	dep	milks	1472:1476	arg1	cow					1468:1470	cow	1468:1470	cow	1468:1470	Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.					
30328901	11	4	theme	study	1423:1427	arg1	limits					1408:1413	the limits	1404:1413	the limits of this study	1404:1427	Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.					
30328901	7	5	theme	pH	927:928	arg1	similar					980:986	similar	980:986	similar	980:986	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	7	5	theme	pH	927:928	arg1	values					930:935	The supernatant pH values	911:935	The supernatant pH values after 6 hours of different treatments	911:973	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	3	6	with	milk	552:555	arg1	DMM+s					588:592	DMM+s	588:592	DMM+s	588:592	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	6	with	milk	552:555	arg1	DMM+s					474:478	DMM+s	474:478	DMM+s	474:478	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	6	with	milk	552:555	arg1	DMM					562:564	DMM	562:564	DMM	562:564	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	6	with	milk	552:555	arg1	DMM+s					538:542	DMM+s	538:542	DMM+s	538:542	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	6	with	milk	552:555	arg1	%					460:460	1%	459:460	1% of sucrose (DMM+s) (positive control)	459:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	6	with	milk	552:555	arg1	DMM					517:519	DMM	517:519	DMM	517:519	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	6	with	milk	552:555	arg1	sucrose					465:471	sucrose	465:471	sucrose (DMM+s) (positive control)	465:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	6	with	milk	552:555	arg1	control					491:497	positive control	482:497	positive control	482:497	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	11	7	contain	have	1478:1481	arg2	potential					1499:1507	some cariogenic potential	1483:1507	some cariogenic potential	1483:1507	Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.					
30328901	11	7	contain	have	1478:1481	arg1	milks					1472:1476	both human and cow milks	1453:1476	both human and cow milks	1453:1476	Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.					
30328901	3	8	theme	positive	482:489	arg1	sucrose					465:471	sucrose	465:471	sucrose (DMM+s) (positive control)	465:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	8	theme	positive	482:489	arg1	control					491:497	positive control	482:497	positive control	482:497	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	9	9	theme	milk	1229:1232	arg1	groups					1234:1239	sucrose and bovine milk groups	1210:1239	sucrose and bovine milk groups	1210:1239	Higher values of total microorganisms count were found for sucrose and bovine milk groups compared to the group supplemented only by DMM.					
30328901	3	10	theme	human	501:505	arg1	milk					507:510	human milk	501:510	human milk with DMM	501:519	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	8	11	theme	pure	1086:1089	arg1	DMM					1091:1093	pure DMM	1086:1093	pure DMM	1086:1093	After 18 hours at rest in pure DMM, an increase in the pH of the supernatant was observed.					
30328901	10	12	theme	milk	1385:1388	arg1	group					1390:1394	human milk group	1379:1394	human milk group	1379:1394	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	11	13	theme	mineral	1554:1560	arg1	loss					1562:1565	mineral loss	1554:1565	mineral loss	1554:1565	Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.					
30328901	11	14	theme	cariogenic	1488:1497	arg1	potential					1499:1507	some cariogenic potential	1483:1507	some cariogenic potential	1483:1507	Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.					
30328901	8	15	from	rest	1078:1081	arg1	hours					1069:1073	18 hours	1066:1073	18 hours at rest in pure DMM	1066:1093	After 18 hours at rest in pure DMM, an increase in the pH of the supernatant was observed.					
30328901	1	16	theme	human	113:117	arg1	milk					119:122	human milk	113:122	human milk	113:122	This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk.					
30328901	8	17	from	hours	1069:1073	arg1	DMM					1091:1093	pure DMM	1086:1093	pure DMM	1086:1093	After 18 hours at rest in pure DMM, an increase in the pH of the supernatant was observed.					
30328901	4	18	dep	variables	621:629	arg1	change					648:653	surface hardness change	631:653	the outcome variables surface hardness change (%SHC)	609:660	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	4	18	dep	variables	621:629	arg1	SHC					657:659	%SHC	656:659	%SHC	656:659	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	0	19	theme	microcosm	55:63	arg1	biofilms					65:72	microcosm biofilms	55:72	microcosm biofilms	55:72	The role of human milk and sucrose on cariogenicity of microcosm biofilms.					
30328901	4	20	theme	outcome	613:619	arg1	variables					621:629	the outcome variables surface hardness change (%SHC)	609:660	the outcome variables surface hardness change (%SHC)	609:660	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	5	21	theme	DMM	806:808	arg1	group					810:814	the DMM group	802:814	the DMM group	802:814	All groups had significantly lower hardness loss compared to the DMM group with 1% of sucrose.					
30328901	5	22	theme	lower	770:774	arg1	loss					785:788	significantly lower hardness loss	756:788	significantly lower hardness loss	756:788	All groups had significantly lower hardness loss compared to the DMM group with 1% of sucrose.					
30328901	4	23	theme	surface	631:637	arg1	change					648:653	surface hardness change	631:653	the outcome variables surface hardness change (%SHC)	609:660	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	4	23	theme	surface	631:637	arg1	SHC					657:659	%SHC	656:659	%SHC	656:659	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	6	24	theme	hardness	896:903	arg1	loss					905:908	higher hardness loss	889:908	higher hardness loss	889:908	Human and bovine milk associated with sucrose showed higher hardness loss.					
30328901	2	25	theme	24-well	335:341	arg1	plates					343:348	24-well plates	335:348	24-well plates	335:348	Microcosm biofilms were grown in enamel discs in 24-well plates.					
30328901	8	26	from	increase	1099:1106	arg1	pH					1115:1116	the pH	1111:1116	the pH of the supernatant	1111:1135	After 18 hours at rest in pure DMM, an increase in the pH of the supernatant was observed.					
30328901	3	27	theme	artificial	411:420	arg1	saliva					422:427	chemically defined artificial saliva	392:427	chemically defined artificial saliva	392:427	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	0	28	theme	milk	18:21	arg1	role					4:7	The role	0:7	The role of human milk and sucrose on cariogenicity of microcosm biofilms.	0:73	The role of human milk and sucrose on cariogenicity of microcosm biofilms.					
30328901	1	29	theme	sucrose	261:267	arg1	cariogenicity					244:256	the cariogenicity	240:256	the cariogenicity of sucrose and bovine milk	240:283	This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk.					
30328901	10	30	theme	milk	1296:1299	arg1	group					1301:1305	Bovine milk group	1289:1305	Bovine milk group	1289:1305	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	4	31	theme	microbiological	663:677	arg1	composition					679:689	microbiological composition	663:689	microbiological composition of biofilms	663:701	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	0	32	theme	sucrose	27:33	arg1	role					4:7	The role	0:7	The role of human milk and sucrose on cariogenicity of microcosm biofilms.	0:73	The role of human milk and sucrose on cariogenicity of microcosm biofilms.					
30328901	1	33	theme	bovine	273:278	arg1	milk					280:283	bovine milk	273:283	bovine milk	273:283	This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk.					
30328901	2	34	theme	Microcosm	286:294	arg1	biofilms					296:303	Microcosm biofilms	286:303	Microcosm biofilms	286:303	Microcosm biofilms were grown in enamel discs in 24-well plates.					
30328901	1	35	theme	biofilms	184:191	arg1	cariogenicity					167:179	the cariogenicity	163:179	the cariogenicity of biofilms	163:191	This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk.					
30328901	3	36	theme	bovine	545:550	arg1	milk					552:555	bovine milk	545:555	bovine milk with DMM	545:564	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	4	37	theme	%	656:656	arg1	change					648:653	surface hardness change	631:653	the outcome variables surface hardness change (%SHC)	609:660	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	4	37	theme	%	656:656	arg1	SHC					657:659	%SHC	656:659	%SHC	656:659	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	9	38	theme	total	1168:1172	arg1	count					1189:1193	total microorganisms count	1168:1193	total microorganisms count	1168:1193	Higher values of total microorganisms count were found for sucrose and bovine milk groups compared to the group supplemented only by DMM.					
30328901	5	39	theme	sucrose	827:833	arg1	sucrose					827:833	sucrose	827:833	sucrose	827:833	All groups had significantly lower hardness loss compared to the DMM group with 1% of sucrose.					
30328901	5	39	theme	sucrose	827:833	arg1	%					822:822	1%	821:822	1% of sucrose	821:833	All groups had significantly lower hardness loss compared to the DMM group with 1% of sucrose.					
30328901	3	40	theme	bovine	571:576	arg1	milk					578:581	bovine milk	571:581	bovine milk with DMM+s	571:592	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	1	41	theme	biofilm	208:214	arg1	model					216:220	a microcosm biofilm model	196:220	a microcosm biofilm model	196:220	This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk.					
30328901	10	42	theme	microorganisms	1347:1360	arg1	amount					1322:1327	greater amount	1314:1327	greater amount of total aciduric microorganisms	1314:1360	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	10	42	theme	microorganisms	1347:1360	arg1	microorganisms					1347:1360	total aciduric microorganisms	1332:1360	total aciduric microorganisms	1332:1360	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	3	43	theme	sucrose	465:471	arg1	sucrose					465:471	sucrose	465:471	sucrose (DMM+s) (positive control)	465:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	43	theme	sucrose	465:471	arg1	%					460:460	1%	459:460	1% of sucrose (DMM+s) (positive control)	459:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	43	theme	sucrose	465:471	arg1	control					491:497	positive control	482:497	positive control	482:497	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	43	theme	sucrose	465:471	arg1	DMM+s					474:478	DMM+s	474:478	DMM+s	474:478	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	10	44	theme	total	1332:1336	arg1	microorganisms					1347:1360	total aciduric microorganisms	1332:1360	total aciduric microorganisms	1332:1360	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	7	45	theme	supernatant	915:925	arg1	similar					980:986	similar	980:986	similar	980:986	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	7	45	theme	supernatant	915:925	arg1	values					930:935	The supernatant pH values	911:935	The supernatant pH values after 6 hours of different treatments	911:973	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	9	46	theme	sucrose	1210:1216	arg1	groups					1234:1239	sucrose and bovine milk groups	1210:1239	sucrose and bovine milk groups	1210:1239	Higher values of total microorganisms count were found for sucrose and bovine milk groups compared to the group supplemented only by DMM.					
30328901	7	47	theme	different	954:962	arg1	treatments					964:973	different treatments	954:973	different treatments	954:973	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	10	48	theme	human	1379:1383	arg1	group					1390:1394	human milk group	1379:1394	human milk group	1379:1394	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	9	49	theme	bovine	1222:1227	arg1	milk					1229:1232	bovine milk	1222:1232	bovine milk	1222:1232	Higher values of total microorganisms count were found for sucrose and bovine milk groups compared to the group supplemented only by DMM.					
30328901	4	50	theme	biofilms	694:701	arg1	pH					708:709	pH	708:709	pH of supernatant	708:724	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	4	50	theme	biofilms	694:701	arg1	composition					679:689	microbiological composition	663:689	microbiological composition of biofilms	663:701	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	4	50	theme	biofilms	694:701	arg1	variables					621:629	the outcome variables surface hardness change (%SHC)	609:660	the outcome variables surface hardness change (%SHC)	609:660	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	2	51	from	discs	326:330	arg1	plates					343:348	24-well plates	335:348	24-well plates	335:348	Microcosm biofilms were grown in enamel discs in 24-well plates.					
30328901	3	52	with	milk	528:531	arg1	DMM+s					588:592	DMM+s	588:592	DMM+s	588:592	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	52	with	milk	528:531	arg1	DMM+s					474:478	DMM+s	474:478	DMM+s	474:478	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	52	with	milk	528:531	arg1	DMM					562:564	DMM	562:564	DMM	562:564	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	52	with	milk	528:531	arg1	DMM+s					538:542	DMM+s	538:542	DMM+s	538:542	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	52	with	milk	528:531	arg1	%					460:460	1%	459:460	1% of sucrose (DMM+s) (positive control)	459:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	52	with	milk	528:531	arg1	DMM					517:519	DMM	517:519	DMM	517:519	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	52	with	milk	528:531	arg1	sucrose					465:471	sucrose	465:471	sucrose (DMM+s) (positive control)	465:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	52	with	milk	528:531	arg1	control					491:497	positive control	482:497	positive control	482:497	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	1	53	theme	milk	280:283	arg1	cariogenicity					244:256	the cariogenicity	240:256	the cariogenicity of sucrose and bovine milk	240:283	This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk.					
30328901	5	54	contain	had	752:754	arg2	loss					785:788	significantly lower hardness loss	756:788	significantly lower hardness loss	756:788	All groups had significantly lower hardness loss compared to the DMM group with 1% of sucrose.					
30328901	5	54	contain	had	752:754	arg1	groups					745:750	All groups	741:750	All groups	741:750	All groups had significantly lower hardness loss compared to the DMM group with 1% of sucrose.					
30328901	11	55	theme	loss	1562:1565	arg1	terms					1545:1549	terms	1545:1549	terms of mineral loss	1545:1565	Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.					
30328901	3	56	with	DMM	387:389	arg1	DMM+s					588:592	DMM+s	588:592	DMM+s	588:592	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	56	with	DMM	387:389	arg1	DMM+s					474:478	DMM+s	474:478	DMM+s	474:478	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	56	with	DMM	387:389	arg1	DMM					562:564	DMM	562:564	DMM	562:564	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	56	with	DMM	387:389	arg1	DMM+s					538:542	DMM+s	538:542	DMM+s	538:542	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	56	with	DMM	387:389	arg1	%					460:460	1%	459:460	1% of sucrose (DMM+s) (positive control)	459:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	56	with	DMM	387:389	arg1	DMM					517:519	DMM	517:519	DMM	517:519	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	56	with	DMM	387:389	arg1	sucrose					465:471	sucrose	465:471	sucrose (DMM+s) (positive control)	465:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	56	with	DMM	387:389	arg1	control					491:497	positive control	482:497	positive control	482:497	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	6	57	theme	bovine	846:851	arg1	milk					853:856	Human and bovine milk	836:856	Human and bovine milk associated with sucrose	836:880	Human and bovine milk associated with sucrose showed higher hardness loss.					
30328901	3	58	with	milk	507:510	arg1	DMM+s					588:592	DMM+s	588:592	DMM+s	588:592	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	58	with	milk	507:510	arg1	DMM+s					474:478	DMM+s	474:478	DMM+s	474:478	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	58	with	milk	507:510	arg1	DMM					562:564	DMM	562:564	DMM	562:564	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	58	with	milk	507:510	arg1	DMM+s					538:542	DMM+s	538:542	DMM+s	538:542	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	58	with	milk	507:510	arg1	%					460:460	1%	459:460	1% of sucrose (DMM+s) (positive control)	459:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	58	with	milk	507:510	arg1	DMM					517:519	DMM	517:519	DMM	517:519	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	58	with	milk	507:510	arg1	sucrose					465:471	sucrose	465:471	sucrose (DMM+s) (positive control)	465:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	58	with	milk	507:510	arg1	control					491:497	positive control	482:497	positive control	482:497	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	1	59	theme	milk	119:122	arg1	effect					103:108	the effect	99:108	the effect	99:108	This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk.					
30328901	6	60	theme	Human	836:840	arg1	milk					853:856	Human and bovine milk	836:856	Human and bovine milk associated with sucrose	836:880	Human and bovine milk associated with sucrose showed higher hardness loss.					
30328901	0	61	theme	biofilms	65:72	arg1	cariogenicity					38:50	cariogenicity	38:50	cariogenicity of microcosm biofilms	38:72	The role of human milk and sucrose on cariogenicity of microcosm biofilms.					
30328901	0	62	from	role	4:7	arg1	cariogenicity					38:50	cariogenicity	38:50	cariogenicity of microcosm biofilms	38:72	The role of human milk and sucrose on cariogenicity of microcosm biofilms.					
30328901	7	63	theme	human	1015:1019	arg1	milk					1021:1024	human milk	1015:1024	human milk	1015:1024	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	7	63	theme	human	1015:1019	arg1	groups					996:1001	the groups sucrose and human milk	992:1024	the groups sucrose and human milk associated with sucrose (p>0.05)	992:1057	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	7	64	dep	groups	996:1001	arg1	sucrose					1003:1009	sucrose	1003:1009	sucrose	1003:1009	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	7	64	dep	groups	996:1001	arg1	milk					1021:1024	human milk	1015:1024	human milk	1015:1024	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	7	64	dep	groups	996:1001	arg1	groups					996:1001	the groups sucrose and human milk	992:1024	the groups sucrose and human milk associated with sucrose (p>0.05)	992:1057	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	5	65	theme	hardness	776:783	arg1	loss					785:788	significantly lower hardness loss	756:788	significantly lower hardness loss	756:788	All groups had significantly lower hardness loss compared to the DMM group with 1% of sucrose.					
30328901	6	66	theme	higher	889:894	arg1	loss					905:908	higher hardness loss	889:908	higher hardness loss	889:908	Human and bovine milk associated with sucrose showed higher hardness loss.					
30328901	11	67	dep	both	1453:1456	arg1	human					1458:1462	human	1458:1462	human	1458:1462	Within the limits of this study, it can be infered that both human and cow milks have some cariogenic potential, although differing from sucrose in terms of mineral loss.					
30328901	0	68	theme	human	12:16	arg1	milk					18:21	human milk	12:21	human milk	12:21	The role of human milk and sucrose on cariogenicity of microcosm biofilms.					
30328901	1	69	from	effect	103:108	arg1	cariogenicity					167:179	the cariogenicity	163:179	the cariogenicity of biofilms	163:191	This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk.					
30328901	8	70	theme	supernatant	1125:1135	arg1	pH					1115:1116	the pH	1111:1116	the pH of the supernatant	1111:1135	After 18 hours at rest in pure DMM, an increase in the pH of the supernatant was observed.					
30328901	7	71	theme	treatments	964:973	arg1	hours					945:949	6 hours	943:949	6 hours of different treatments	943:973	The supernatant pH values after 6 hours of different treatments were similar for the groups sucrose and human milk associated with sucrose (p>0.05).					
30328901	3	72	dep	studied	378:384	arg1	DMM					450:452	DMM	450:452	DMM with 1% of sucrose (DMM+s) (positive control)	450:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	72	dep	studied	378:384	arg1	milk					528:531	human milk	522:531	human milk with DMM+s	522:542	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	72	dep	studied	378:384	arg1	DMM					387:389	DMM	387:389	DMM (chemically defined artificial saliva - negative control)	387:447	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	72	dep	studied	378:384	arg1	milk					507:510	human milk	501:510	human milk with DMM	501:519	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	72	dep	studied	378:384	arg1	milk					552:555	bovine milk	545:555	bovine milk with DMM	545:564	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	72	dep	studied	378:384	arg1	milk					578:581	bovine milk	571:581	bovine milk with DMM+s	571:592	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	73	theme	human	522:526	arg1	milk					528:531	human milk	522:531	human milk with DMM+s	522:542	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	9	74	theme	count	1189:1193	arg1	values					1158:1163	Higher values	1151:1163	Higher values of total microorganisms count	1151:1193	Higher values of total microorganisms count were found for sucrose and bovine milk groups compared to the group supplemented only by DMM.					
30328901	3	75	theme	defined	403:409	arg1	saliva					422:427	chemically defined artificial saliva	392:427	chemically defined artificial saliva	392:427	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	76	dep	DMM	387:389	arg1	control					440:446	negative control	431:446	negative control	431:446	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	76	dep	DMM	387:389	arg1	saliva					422:427	chemically defined artificial saliva	392:427	chemically defined artificial saliva	392:427	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	77	theme	growth	355:360	arg1	conditions					362:371	Six growth conditions	351:371	Six growth conditions	351:371	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	10	78	theme	Bovine	1289:1294	arg1	group					1301:1305	Bovine milk group	1289:1305	Bovine milk group	1289:1305	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	3	79	with	milk	578:581	arg1	DMM+s					588:592	DMM+s	588:592	DMM+s	588:592	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	79	with	milk	578:581	arg1	DMM+s					474:478	DMM+s	474:478	DMM+s	474:478	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	79	with	milk	578:581	arg1	DMM					562:564	DMM	562:564	DMM	562:564	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	79	with	milk	578:581	arg1	DMM+s					538:542	DMM+s	538:542	DMM+s	538:542	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	79	with	milk	578:581	arg1	%					460:460	1%	459:460	1% of sucrose (DMM+s) (positive control)	459:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	79	with	milk	578:581	arg1	DMM					517:519	DMM	517:519	DMM	517:519	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	79	with	milk	578:581	arg1	sucrose					465:471	sucrose	465:471	sucrose (DMM+s) (positive control)	465:498	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	3	79	with	milk	578:581	arg1	control					491:497	positive control	482:497	positive control	482:497	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	9	80	theme	Higher	1151:1156	arg1	values					1158:1163	Higher values	1151:1163	Higher values of total microorganisms count	1151:1193	Higher values of total microorganisms count were found for sucrose and bovine milk groups compared to the group supplemented only by DMM.					
30328901	4	81	theme	hardness	639:646	arg1	change					648:653	surface hardness change	631:653	the outcome variables surface hardness change (%SHC)	609:660	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	4	81	theme	hardness	639:646	arg1	SHC					657:659	%SHC	656:659	%SHC	656:659	After 5 days, the outcome variables surface hardness change (%SHC), microbiological composition of biofilms, and pH of supernatant were analyzed.					
30328901	3	82	theme	negative	431:438	arg1	control					440:446	negative control	431:446	negative control	431:446	Six growth conditions were studied: DMM (chemically defined artificial saliva - negative control), DMM with 1% of sucrose (DMM+s) (positive control), human milk with DMM, human milk with DMM+s, bovine milk with DMM, and bovine milk with DMM+s.					
30328901	10	83	theme	greater	1314:1320	arg1	amount					1322:1327	greater amount	1314:1327	greater amount of total aciduric microorganisms	1314:1360	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	10	83	theme	greater	1314:1320	arg1	microorganisms					1347:1360	total aciduric microorganisms	1332:1360	total aciduric microorganisms	1332:1360	Bovine milk group showed greater amount of total aciduric microorganisms in comparison to human milk group.					
30328901	1	84	theme	microcosm	198:206	arg1	model					216:220	a microcosm biofilm model	196:220	a microcosm biofilm model	196:220	This study investigated the effect of human milk, alone and associated with sucrose, in the cariogenicity of biofilms in a microcosm biofilm model and compared with the cariogenicity of sucrose and bovine milk.					
30328901	2	85	theme	enamel	319:324	arg1	discs					326:330	enamel discs	319:330	enamel discs in 24-well plates	319:348	Microcosm biofilms were grown in enamel discs in 24-well plates.					
30328901	9	86	theme	microorganisms	1174:1187	arg1	count					1189:1193	total microorganisms count	1168:1193	total microorganisms count	1168:1193	Higher values of total microorganisms count were found for sucrose and bovine milk groups compared to the group supplemented only by DMM.					
30904298	13	0	theme	other	2484:2488	arg1	constituents					2500:2511	other colostrum constituents	2484:2511	other colostrum constituents	2484:2511	In conclusion, IgG and concentrations of other colostrum constituents showed a wide range in goats and ewes and were mainly affected by the type of breed.					
30904298	8	1	dep	concentrations	1671:1684	arg1	mg/mL					1713:1717	17.9 ± 7.3 and 20.2 ± 8.0 mg/mL	1687:1717	17.9 ± 7.3 and 20.2 ± 8.0 mg/mL	1687:1717	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	4	2	theme	Brown-Headed	761:772	arg1	Meat					774:777	Brown-Headed Meat	761:777	Brown-Headed Meat	761:777	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	2	theme	Brown-Headed	761:772	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	12	3	dep	associated	2205:2214	arg1	twins					2364:2368	twins	2364:2368	twins	2364:2368	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	12	3	dep	associated	2205:2214	arg1	singletons					2349:2358	singletons	2349:2358	singletons	2349:2358	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	7	4	dep	Boer	1441:1444	arg1	meat-type					1447:1455	meat-type	1447:1455	meat-type	1447:1455	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	8	5	theme	44.2	1798:1801	arg1	±					1803:1803	±	1803:1803	±	1803:1803	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	3	6	from	production	542:551	arg1	Germany					572:578	Germany	572:578	Germany	572:578	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	3	6	from	production	542:551	arg1	Switzerland					556:566	Switzerland	556:566	Switzerland	556:566	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	12	7	theme	caprine	2324:2330	arg1	colostrum					2332:2340	ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum	2272:2340	colostrum	2332:2340	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	8	8	theme	15.7	1805:1808	arg1	±					1803:1803	±	1803:1803	±	1803:1803	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	5	9	theme	lactose	1156:1162	arg1	contents					1164:1171	lactose contents	1156:1171	lactose contents	1156:1171	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	12	10	dep	singletons	2285:2294	arg1	i.e.					2279:2282	i.e.	2279:2282	i.e.	2279:2282	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	7	11	theme	±	1482:1482	arg1	SD					1484:1485	mean ± SD	1477:1485	61.0 ± 10.3 mg/mL; mean ± SD	1458:1485	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	3	12	theme	different	485:493	arg1	breeds					510:515	different goat and sheep breeds	485:515	different goat and sheep breeds raised for milk and meat production in Switzerland and Germany	485:578	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	4	13	theme	Friesian	785:792	arg1	Milk					794:797	East Friesian Milk	780:797	East Friesian Milk	780:797	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	13	theme	Friesian	785:792	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	1	14	theme	important	189:197	arg1	source					206:211	an important energy source	186:211	an important energy source for newborn lambs and goat kids	186:243	Colostrum represents the sole source to acquire humoral immunity and is an important energy source for newborn lambs and goat kids.					
30904298	3	15	theme	breeds	510:515	arg1	composition					470:480	the colostrum composition	456:480	the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany	456:578	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	7	16	located	found	1432:1436	arg1	goats					1389:1393	goats	1389:1393	goats	1389:1393	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	7	16	located	found	1432:1436	arg2	concentrations					1412:1425	the highest IgG concentrations	1396:1425	the highest IgG concentrations	1396:1425	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	7	16	located	found	1432:1436	arg1	Boer					1441:1444	Boer	1441:1444	Boer	1441:1444	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	13	17	theme	constituents	2500:2511	arg1	concentrations					2466:2479	concentrations	2466:2479	concentrations of other colostrum constituents	2466:2511	In conclusion, IgG and concentrations of other colostrum constituents showed a wide range in goats and ewes and were mainly affected by the type of breed.					
30904298	13	17	theme	constituents	2500:2511	arg1	IgG					2458:2460	IgG	2458:2460	IgG	2458:2460	In conclusion, IgG and concentrations of other colostrum constituents showed a wide range in goats and ewes and were mainly affected by the type of breed.					
30904298	8	18	theme	East	1602:1605	arg1	Milk					1616:1619	East Friesian Milk and Lacaune Dairy	1602:1637	Milk	1616:1619	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	2	19	dep	contents	288:295	arg1	i.e.					278:281	i.e.	278:281	i.e.	278:281	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	9	20	theme	lowest	1822:1827	arg1	fat					1829:1831	The lowest fat	1818:1831	The lowest fat	1818:1831	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	12	21	from	concentrations	2254:2267	arg1	colostrum					2332:2340	ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum	2272:2340	colostrum	2332:2340	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	12	21	from	concentrations	2254:2267	arg1	ovine					2272:2276	ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum	2272:2340	ovine	2272:2276	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	7	22	located	observed	1523:1530	arg1	mg/mL					1584:1588	milk-type; 26.5 ± 12.5 mg/mL	1561:1588	milk-type; 26.5 ± 12.5 mg/mL	1561:1588	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	7	22	located	observed	1523:1530	arg2	concentrations					1503:1516	the lowest concentrations	1492:1516	the lowest concentrations	1492:1516	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	7	22	located	observed	1523:1530	arg1	Edelziege					1550:1558	Edelziege	1550:1558	Edelziege	1550:1558	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	11	23	theme	fat	2076:2078	arg1	content					2080:2086	colostral fat content	2066:2086	colostral fat content	2066:2086	In contrast, colostral fat content was higher in ewes bearing twins and triplets than in those carrying singletons.					
30904298	5	24	theme	10	1077:1078	arg1	min					1088:1090	10 and 390 min	1077:1090	min	1088:1090	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	8	25	theme	Lacaune	1625:1631	arg1	Dairy					1633:1637	East Friesian Milk and Lacaune Dairy	1602:1637	Dairy	1633:1637	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	8	26	theme	±	1692:1692	arg1	mg/mL					1713:1717	17.9 ± 7.3 and 20.2 ± 8.0 mg/mL	1687:1717	17.9 ± 7.3 and 20.2 ± 8.0 mg/mL	1687:1717	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	0	27	from	content	17:23	arg1	Switzerland					89:99	Switzerland	89:99	Switzerland	89:99	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	0	27	from	content	17:23	arg1	Germany					105:111	Germany	105:111	Germany	105:111	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	12	28	theme	lower	2240:2244	arg1	concentrations					2254:2267	lower lactose concentrations	2240:2267	lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum	2240:2340	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	0	29	theme	sheep	73:77	arg1	breeds					79:84	different goat and sheep breeds	54:84	different goat and sheep breeds	54:84	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	12	30	from	protein	2228:2234	arg1	colostrum					2332:2340	ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum	2272:2340	colostrum	2332:2340	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	12	30	from	protein	2228:2234	arg1	ovine					2272:2276	ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum	2272:2340	ovine	2272:2276	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	4	31	theme	Blackheaded	807:817	arg1	Mutton					819:824	German Blackheaded Mutton	800:824	German Blackheaded Mutton	800:824	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	31	theme	Blackheaded	807:817	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	9	32	located	observed	1914:1921	arg1	sheep					1976:1980	Lacaune Dairy sheep	1962:1980	Lacaune Dairy sheep	1962:1980	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	9	32	located	observed	1914:1921	arg2	concentrations					1845:1858	The lowest fat and protein concentrations	1818:1858	The lowest fat and protein concentrations	1818:1858	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	9	32	located	observed	1914:1921	arg2	concentrations					1894:1907	highest lactose concentrations	1878:1907	highest lactose concentrations	1878:1907	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	9	32	located	observed	1914:1921	arg1	colostrum					1926:1934	colostrum	1926:1934	colostrum of East Friesian Milk	1926:1956	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	5	33	theme	=	1062:1062	arg1	n					1060:1060	n = 116	1060:1066	n = 116	1060:1066	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	5	33	theme	=	1062:1062	arg1	goats					1053:1057	goats	1053:1057	goats (n = 116)	1053:1067	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	8	34	theme	lowest	1650:1655	arg1	concentrations					1671:1684	the lowest colostral IgG concentrations	1646:1684	the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively)	1646:1732	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	6	35	from	mg/mL	1272:1276	arg1	ewes					1281:1284	ewes	1281:1284	ewes	1281:1284	Colostral IgG concentrations varied between 4.8 and 75.0 mg/mL in goats, and between 6.2 and 65.4 mg/mL in ewes, and the time interval between milking and parturition did not affect colostral IgG concentrations.					
30904298	12	36	theme	litter	2430:2435	arg1	size					2437:2440	litter size	2430:2440	litter size	2430:2440	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	9	37	theme	lactose	1886:1892	arg1	concentrations					1894:1907	highest lactose concentrations	1878:1907	highest lactose concentrations	1878:1907	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	8	38	theme	IgG	1667:1669	arg1	concentrations					1671:1684	the lowest colostral IgG concentrations	1646:1684	the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively)	1646:1732	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	4	39	dep	breeds	753:758	arg1	Milk					794:797	East Friesian Milk	780:797	East Friesian Milk	780:797	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	39	dep	breeds	753:758	arg1	Heath					839:843	Gray Horned Heath	827:843	Gray Horned Heath	827:843	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	39	dep	breeds	753:758	arg1	Land					868:871	Merino Land	861:871	Merino Land	861:871	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	39	dep	breeds	753:758	arg1	Meat					774:777	Brown-Headed Meat	761:777	Brown-Headed Meat	761:777	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	39	dep	breeds	753:758	arg1	Blacknose					951:959	Blacknose	951:959	Blacknose	951:959	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	39	dep	breeds	753:758	arg1	Mutton					819:824	German Blackheaded Mutton	800:824	German Blackheaded Mutton	800:824	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	39	dep	breeds	753:758	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	39	dep	breeds	753:758	arg1	Dairy					854:858	Lacaune Dairy	846:858	Lacaune Dairy	846:858	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	39	dep	breeds	753:758	arg1	Alpine					932:937	Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine	874:937	Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine	874:937	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	6	40	theme	colostral	1356:1364	arg1	concentrations					1370:1383	colostral IgG concentrations	1356:1383	colostral IgG concentrations	1356:1383	Colostral IgG concentrations varied between 4.8 and 75.0 mg/mL in goats, and between 6.2 and 65.4 mg/mL in ewes, and the time interval between milking and parturition did not affect colostral IgG concentrations.					
30904298	7	41	theme	IgG	1408:1410	arg1	concentrations					1412:1425	the highest IgG concentrations	1396:1425	the highest IgG concentrations	1396:1425	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	2	42	theme	litter	382:387	arg1	size					389:392	litter size	382:392	litter size	382:392	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	4	43	theme	Horned	832:837	arg1	Heath					839:843	Gray Horned Heath	827:843	Gray Horned Heath	827:843	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	43	theme	Horned	832:837	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	0	44	theme	Immunoglobulin	0:13	arg1	content					17:23	Immunoglobulin G content	0:23	Immunoglobulin G content	0:23	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	5	45	theme	colostrum	997:1005	arg1	samples					1007:1013	First colostrum samples	991:1013	First colostrum samples	991:1013	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	7	46	dep	meat-type	1447:1455	arg1	mg/mL					1470:1474	61.0 ± 10.3 mg/mL	1458:1474	61.0 ± 10.3 mg/mL; mean ± SD	1458:1485	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	5	47	theme	390	1084:1086	arg1	min					1088:1090	10 and 390 min	1077:1090	min	1088:1090	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	6	48	theme	IgG	1184:1186	arg1	concentrations					1188:1201	Colostral IgG concentrations	1174:1201	Colostral IgG concentrations	1174:1201	Colostral IgG concentrations varied between 4.8 and 75.0 mg/mL in goats, and between 6.2 and 65.4 mg/mL in ewes, and the time interval between milking and parturition did not affect colostral IgG concentrations.					
30904298	2	49	theme	various	347:353	arg1	factors					355:361	various factors	347:361	various factors such as parity and litter size and, potentially, by breed	347:419	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	2	49	theme	various	347:353	arg1	parity					371:376	parity	371:376	parity	371:376	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	2	49	theme	various	347:353	arg1	size					389:392	litter size	382:392	litter size	382:392	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	2	49	theme	various	347:353	arg1	potentially					399:409	potentially	399:409	potentially	399:409	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	0	50	theme	colostrum	29:37	arg1	composition					39:49	colostrum composition	29:49	colostrum composition	29:49	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	12	51	dep	singletons	2349:2358	arg1	i.e.					2343:2346	i.e.	2343:2346	i.e.	2343:2346	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	4	52	theme	Merino	861:866	arg1	Land					868:871	Merino Land	861:871	Merino Land	861:871	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	52	theme	Merino	861:866	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	0	53	theme	goat	64:67	arg1	breeds					79:84	different goat and sheep breeds	54:84	different goat and sheep breeds	54:84	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	2	54	theme	fat	305:307	arg1	contents					288:295	the contents	284:295	the contents of IgG, fat, protein, and lactose	284:329	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	4	55	theme	Swiss	920:924	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	55	theme	Swiss	920:924	arg1	Alpine					932:937	Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine	874:937	Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine	874:937	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	2	56	theme	IgG	300:302	arg1	contents					288:295	the contents	284:295	the contents of IgG, fat, protein, and lactose	284:329	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	5	57	theme	=	1042:1042	arg1	n					1040:1040	n = 100	1040:1046	n = 100	1040:1046	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	5	57	theme	=	1042:1042	arg1	ewes					1034:1037	ewes	1034:1037	ewes (n = 100)	1034:1047	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	7	58	theme	milk-type	1561:1569	arg1	mg/mL					1584:1588	milk-type; 26.5 ± 12.5 mg/mL	1561:1588	milk-type; 26.5 ± 12.5 mg/mL	1561:1588	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	7	58	theme	milk-type	1561:1569	arg1	Edelziege					1550:1558	Edelziege	1550:1558	Edelziege	1550:1558	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	10	59	theme	Parity	1983:1988	arg1	number					1990:1995	Parity number	1983:1995	Parity number	1983:1995	Parity number did not affect colostrum composition in sheep or goats.					
30904298	12	60	theme	Increasing	2169:2178	arg1	size					2187:2190	Increasing litter size	2169:2190	Increasing litter size	2169:2190	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	3	61	theme	present	429:435	arg1	study					437:441	the present study	425:441	the present study	425:441	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	7	62	theme	lowest	1496:1501	arg1	concentrations					1503:1516	the lowest concentrations	1492:1516	the lowest concentrations	1492:1516	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	7	63	dep	mg/mL	1470:1474	arg1	SD					1484:1485	mean ± SD	1477:1485	61.0 ± 10.3 mg/mL; mean ± SD	1458:1485	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	2	64	theme	protein	310:316	arg1	contents					288:295	the contents	284:295	the contents of IgG, fat, protein, and lactose	284:329	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	1	65	theme	goat	235:238	arg1	kids					240:243	goat kids	235:243	goat kids	235:243	Colostrum represents the sole source to acquire humoral immunity and is an important energy source for newborn lambs and goat kids.					
30904298	13	66	theme	colostrum	2490:2498	arg1	constituents					2500:2511	other colostrum constituents	2484:2511	other colostrum constituents	2484:2511	In conclusion, IgG and concentrations of other colostrum constituents showed a wide range in goats and ewes and were mainly affected by the type of breed.					
30904298	8	67	theme	Land	1786:1789	arg1	mg/mL					1810:1814	44.2 ± 15.7 mg/mL	1798:1814	44.2 ± 15.7 mg/mL	1798:1814	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	8	67	theme	Land	1786:1789	arg1	breed					1791:1795	the Merino Land breed	1775:1795	the Merino Land breed (44.2 ± 15.7 mg/mL)	1775:1815	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	4	68	theme	sheep	747:751	arg1	Milk					794:797	East Friesian Milk	780:797	East Friesian Milk	780:797	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	68	theme	sheep	747:751	arg1	Heath					839:843	Gray Horned Heath	827:843	Gray Horned Heath	827:843	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	68	theme	sheep	747:751	arg1	Land					868:871	Merino Land	861:871	Merino Land	861:871	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	68	theme	sheep	747:751	arg1	Meat					774:777	Brown-Headed Meat	761:777	Brown-Headed Meat	761:777	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	68	theme	sheep	747:751	arg1	Blacknose					951:959	Blacknose	951:959	Blacknose	951:959	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	68	theme	sheep	747:751	arg1	Mutton					819:824	German Blackheaded Mutton	800:824	German Blackheaded Mutton	800:824	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	68	theme	sheep	747:751	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	68	theme	sheep	747:751	arg1	Dairy					854:858	Lacaune Dairy	846:858	Lacaune Dairy	846:858	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	68	theme	sheep	747:751	arg1	Alpine					932:937	Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine	874:937	Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine	874:937	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	9	69	theme	Friesian	1944:1951	arg1	Milk					1953:1956	East Friesian Milk	1939:1956	East Friesian Milk	1939:1956	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	2	70	theme	colostrum	255:263	arg1	composition					265:275	colostrum composition	255:275	colostrum composition (i.e., the contents of IgG, fat, protein, and lactose)	255:330	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	1	71	theme	humoral	162:168	arg1	immunity					170:177	humoral immunity	162:177	humoral immunity	162:177	Colostrum represents the sole source to acquire humoral immunity and is an important energy source for newborn lambs and goat kids.					
30904298	7	72	theme	mean	1477:1480	arg1	SD					1484:1485	mean ± SD	1477:1485	61.0 ± 10.3 mg/mL; mean ± SD	1458:1485	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	3	73	theme	colostrum	460:468	arg1	composition					470:480	the colostrum composition	456:480	the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany	456:578	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	12	74	dep	ovine	2272:2276	arg1	singletons					2285:2294	singletons	2285:2294	singletons	2285:2294	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	12	74	dep	ovine	2272:2276	arg1	twins					2300:2304	twins	2300:2304	twins	2300:2304	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	4	75	theme	East	780:783	arg1	Milk					794:797	East Friesian Milk	780:797	East Friesian Milk	780:797	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	75	theme	East	780:783	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	8	76	located	observed	1763:1770	arg2	values					1751:1756	the highest values	1739:1756	the highest values	1739:1756	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	8	76	located	observed	1763:1770	arg1	mg/mL					1810:1814	44.2 ± 15.7 mg/mL	1798:1814	44.2 ± 15.7 mg/mL	1798:1814	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	8	76	located	observed	1763:1770	arg1	breed					1791:1795	the Merino Land breed	1775:1795	the Merino Land breed (44.2 ± 15.7 mg/mL)	1775:1815	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	3	77	theme	sheep	504:508	arg1	breeds					510:515	different goat and sheep breeds	485:515	different goat and sheep breeds raised for milk and meat production in Switzerland and Germany	485:578	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	1	78	theme	energy	199:204	arg1	source					206:211	an important energy source	186:211	an important energy source for newborn lambs and goat kids	186:243	Colostrum represents the sole source to acquire humoral immunity and is an important energy source for newborn lambs and goat kids.					
30904298	13	79	theme	breed	2591:2595	arg1	type					2583:2586	the type	2579:2586	the type of breed	2579:2595	In conclusion, IgG and concentrations of other colostrum constituents showed a wide range in goats and ewes and were mainly affected by the type of breed.					
30904298	8	80	theme	Friesian	1607:1614	arg1	Milk					1616:1619	East Friesian Milk and Lacaune Dairy	1602:1637	Milk	1616:1619	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	3	81	theme	goat	495:498	arg1	breeds					510:515	different goat and sheep breeds	485:515	different goat and sheep breeds raised for milk and meat production in Switzerland and Germany	485:578	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	10	82	from	composition	2022:2032	arg1	goats					2046:2050	goats	2046:2050	goats	2046:2050	Parity number did not affect colostrum composition in sheep or goats.					
30904298	10	82	from	composition	2022:2032	arg1	sheep					2037:2041	sheep	2037:2041	sheep	2037:2041	Parity number did not affect colostrum composition in sheep or goats.					
30904298	3	83	theme	milk	528:531	arg1	production					542:551	milk and meat production	528:551	milk and meat production in Switzerland and Germany	528:578	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	11	84	theme	colostral	2066:2074	arg1	content					2080:2086	colostral fat content	2066:2086	colostral fat content	2066:2086	In contrast, colostral fat content was higher in ewes bearing twins and triplets than in those carrying singletons.					
30904298	9	85	theme	fat	1829:1831	arg1	concentrations					1845:1858	The lowest fat and protein concentrations	1818:1858	The lowest fat and protein concentrations	1818:1858	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	6	86	theme	time	1295:1298	arg1	interval					1300:1307	the time interval	1291:1307	the time interval between milking and parturition	1291:1339	Colostral IgG concentrations varied between 4.8 and 75.0 mg/mL in goats, and between 6.2 and 65.4 mg/mL in ewes, and the time interval between milking and parturition did not affect colostral IgG concentrations.					
30904298	1	87	theme	sole	139:142	arg1	source					144:149	the sole source	135:149	the sole source to acquire humoral immunity	135:177	Colostrum represents the sole source to acquire humoral immunity and is an important energy source for newborn lambs and goat kids.					
30904298	4	88	theme	Gray	827:830	arg1	Heath					839:843	Gray Horned Heath	827:843	Gray Horned Heath	827:843	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	88	theme	Gray	827:830	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	12	89	theme	lactose	2246:2252	arg1	concentrations					2254:2267	lower lactose concentrations	2240:2267	lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum	2240:2340	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	4	90	theme	goat	585:588	arg1	Peacock					689:695	Peacock	689:695	Peacock	689:695	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	90	theme	goat	585:588	arg1	Appenzell					612:620	Appenzell	612:620	Appenzell	612:620	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	90	theme	goat	585:588	arg1	Blackneck					729:737	Blackneck	729:737	Blackneck	729:737	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	90	theme	goat	585:588	arg1	breeds					590:595	Ten goat breeds	581:595	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck)	581:738	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	90	theme	goat	585:588	arg1	Grisons					672:678	Grisons	672:678	Grisons	672:678	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	90	theme	goat	585:588	arg1	Toggenburg					706:715	Toggenburg	706:715	Toggenburg	706:715	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	90	theme	goat	585:588	arg1	Edelziege					644:652	Edelziege	644:652	Edelziege	644:652	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	9	91	theme	protein	1837:1843	arg1	concentrations					1845:1858	The lowest fat and protein concentrations	1818:1858	The lowest fat and protein concentrations	1818:1858	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	0	92	theme	breeds	79:84	arg1	content					17:23	Immunoglobulin G content	0:23	Immunoglobulin G content	0:23	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	0	92	theme	breeds	79:84	arg1	composition					39:49	colostrum composition	29:49	colostrum composition	29:49	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	13	93	theme	wide	2522:2525	arg1	range					2527:2531	a wide range	2520:2531	a wide range	2520:2531	In conclusion, IgG and concentrations of other colostrum constituents showed a wide range in goats and ewes and were mainly affected by the type of breed.					
30904298	2	94	dep	composition	265:275	arg1	contents					288:295	the contents	284:295	the contents of IgG, fat, protein, and lactose	284:329	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	8	95	theme	colostral	1657:1665	arg1	concentrations					1671:1684	the lowest colostral IgG concentrations	1646:1684	the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively)	1646:1732	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	4	96	theme	German	800:805	arg1	Mutton					819:824	German Blackheaded Mutton	800:824	German Blackheaded Mutton	800:824	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	96	theme	German	800:805	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	12	97	theme	higher	2221:2226	arg1	protein					2228:2234	higher protein	2221:2234	higher protein	2221:2234	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	9	98	theme	highest	1878:1884	arg1	concentrations					1894:1907	highest lactose concentrations	1878:1907	highest lactose concentrations	1878:1907	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	6	99	theme	IgG	1366:1368	arg1	concentrations					1370:1383	colostral IgG concentrations	1356:1383	colostral IgG concentrations	1356:1383	Colostral IgG concentrations varied between 4.8 and 75.0 mg/mL in goats, and between 6.2 and 65.4 mg/mL in ewes, and the time interval between milking and parturition did not affect colostral IgG concentrations.					
30904298	4	100	dep	breeds	590:595	arg1	Peacock					689:695	Peacock	689:695	Peacock	689:695	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	100	dep	breeds	590:595	arg1	Appenzell					612:620	Appenzell	612:620	Appenzell	612:620	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	100	dep	breeds	590:595	arg1	Blackneck					729:737	Blackneck	729:737	Blackneck	729:737	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	100	dep	breeds	590:595	arg1	Chamois-colored					655:669	Chamois-colored	655:669	Chamois-colored	655:669	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	100	dep	breeds	590:595	arg1	breeds					590:595	Ten goat breeds	581:595	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck)	581:738	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	100	dep	breeds	590:595	arg1	Grisons					672:678	Grisons	672:678	Grisons	672:678	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	100	dep	breeds	590:595	arg1	Toggenburg					706:715	Toggenburg	706:715	Toggenburg	706:715	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	100	dep	breeds	590:595	arg1	Bunte					629:633	Bunte	629:633	Bunte	629:633	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	100	dep	breeds	590:595	arg1	Edelziege					644:652	Edelziege	644:652	Edelziege	644:652	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	10	101	theme	colostrum	2012:2020	arg1	composition					2022:2032	colostrum composition	2012:2032	colostrum composition in sheep or goats	2012:2050	Parity number did not affect colostrum composition in sheep or goats.					
30904298	0	102	from	composition	39:49	arg1	Switzerland					89:99	Switzerland	89:99	Switzerland	89:99	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	0	102	from	composition	39:49	arg1	Germany					105:111	Germany	105:111	Germany	105:111	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	0	103	theme	G	15:15	arg1	content					17:23	Immunoglobulin G content	0:23	Immunoglobulin G content	0:23	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	12	104	theme	colostral	2380:2388	arg1	concentrations					2394:2407	colostral IgG concentrations	2380:2407	colostral IgG concentrations	2380:2407	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	2	105	theme	lactose	323:329	arg1	contents					288:295	the contents	284:295	the contents of IgG, fat, protein, and lactose	284:329	However, colostrum composition (i.e., the contents of IgG, fat, protein, and lactose) is affected by various factors such as parity and litter size and, potentially, by breed.					
30904298	7	106	theme	highest	1400:1406	arg1	concentrations					1412:1425	the highest IgG concentrations	1396:1425	the highest IgG concentrations	1396:1425	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	12	107	theme	litter	2180:2185	arg1	size					2187:2190	Increasing litter size	2169:2190	Increasing litter size	2169:2190	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30904298	9	108	theme	Dairy	1970:1974	arg1	sheep					1976:1980	Lacaune Dairy sheep	1962:1980	Lacaune Dairy sheep	1962:1980	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	6	109	theme	Colostral	1174:1182	arg1	concentrations					1188:1201	Colostral IgG concentrations	1174:1201	Colostral IgG concentrations	1174:1201	Colostral IgG concentrations varied between 4.8 and 75.0 mg/mL in goats, and between 6.2 and 65.4 mg/mL in ewes, and the time interval between milking and parturition did not affect colostral IgG concentrations.					
30904298	7	110	theme	±	1577:1577	arg1	mg/mL					1584:1588	milk-type; 26.5 ± 12.5 mg/mL	1561:1588	milk-type; 26.5 ± 12.5 mg/mL	1561:1588	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	7	110	theme	±	1577:1577	arg1	Edelziege					1550:1558	Edelziege	1550:1558	Edelziege	1550:1558	In goats, the highest IgG concentrations were found in Boer (meat-type; 61.0 ± 10.3 mg/mL; mean ± SD) and the lowest concentrations were observed in Bunte Deutsche Edelziege (milk-type; 26.5 ± 12.5 mg/mL).					
30904298	8	111	theme	highest	1743:1749	arg1	values					1751:1756	the highest values	1739:1756	the highest values	1739:1756	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	4	112	theme	Lacaune	846:852	arg1	Dairy					854:858	Lacaune Dairy	846:858	Lacaune Dairy	846:858	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	112	theme	Lacaune	846:852	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	3	113	theme	meat	537:540	arg1	production					542:551	milk and meat production	528:551	milk and meat production in Switzerland and Germany	528:578	In the present study, we examined the colostrum composition of different goat and sheep breeds raised for milk and meat production in Switzerland and Germany.					
30904298	9	114	theme	East	1939:1942	arg1	Milk					1953:1956	East Friesian Milk	1939:1956	East Friesian Milk	1939:1956	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	0	115	theme	different	54:62	arg1	breeds					79:84	different goat and sheep breeds	54:84	different goat and sheep breeds	54:84	Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany.					
30904298	9	116	theme	Milk	1953:1956	arg1	colostrum					1926:1934	colostrum	1926:1934	colostrum of East Friesian Milk	1926:1956	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	9	116	theme	Milk	1953:1956	arg1	sheep					1976:1980	Lacaune Dairy sheep	1962:1980	Lacaune Dairy sheep	1962:1980	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	5	117	theme	First	991:995	arg1	samples					1007:1013	First colostrum samples	991:1013	First colostrum samples	991:1013	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	11	118	contain	carrying	2148:2155	arg2	singletons					2157:2166	singletons	2157:2166	singletons	2157:2166	In contrast, colostral fat content was higher in ewes bearing twins and triplets than in those carrying singletons.					
30904298	11	118	contain	carrying	2148:2155	arg1	those					2142:2146	those	2142:2146	those	2142:2146	In contrast, colostral fat content was higher in ewes bearing twins and triplets than in those carrying singletons.					
30904298	5	119	theme	total	1127:1131	arg1	IgG					1133:1135	total IgG	1127:1135	total IgG	1127:1135	First colostrum samples were obtained from ewes (n = 100) and goats (n = 116) between 10 and 390 min after parturition and analyzed for total IgG, fat, protein, and lactose contents.					
30904298	9	120	theme	Lacaune	1962:1968	arg1	sheep					1976:1980	Lacaune Dairy sheep	1962:1980	Lacaune Dairy sheep	1962:1980	The lowest fat and protein concentrations and concomitantly highest lactose concentrations were observed in colostrum of East Friesian Milk and Lacaune Dairy sheep.					
30904298	1	121	theme	newborn	217:223	arg1	lambs					225:229	newborn lambs	217:229	newborn lambs	217:229	Colostrum represents the sole source to acquire humoral immunity and is an important energy source for newborn lambs and goat kids.					
30904298	6	122	from	mg/mL	1231:1235	arg1	goats					1240:1244	goats	1240:1244	goats	1240:1244	Colostral IgG concentrations varied between 4.8 and 75.0 mg/mL in goats, and between 6.2 and 65.4 mg/mL in ewes, and the time interval between milking and parturition did not affect colostral IgG concentrations.					
30904298	8	123	theme	Merino	1779:1784	arg1	mg/mL					1810:1814	44.2 ± 15.7 mg/mL	1798:1814	44.2 ± 15.7 mg/mL	1798:1814	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	8	123	theme	Merino	1779:1784	arg1	breed					1791:1795	the Merino Land breed	1775:1795	the Merino Land breed (44.2 ± 15.7 mg/mL)	1775:1815	In sheep, East Friesian Milk and Lacaune Dairy showed the lowest colostral IgG concentrations (17.9 ± 7.3 and 20.2 ± 8.0 mg/mL, respectively), and the highest values were observed in the Merino Land breed (44.2 ± 15.7 mg/mL).					
30904298	4	124	theme	White	926:930	arg1	breeds					753:758	10 sheep breeds	744:758	10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose)	744:960	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	4	124	theme	White	926:930	arg1	Alpine					932:937	Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine	874:937	Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine	874:937	Ten goat breeds (Anglo-Nubian, Appenzell, Boer, Bunte Deutsche Edelziege, Chamois-colored, Grisons Striped, Peacock, Saanen, Toggenburg, and Valais Blackneck) and 10 sheep breeds (Brown-Headed Meat, East Friesian Milk, German Blackheaded Mutton, Gray Horned Heath, Lacaune Dairy, Merino Land, Swiss Black-Brown Mountain, Swiss Charollais, Swiss White Alpine, and Valais Blacknose) were involved in this study.					
30904298	12	125	theme	IgG	2390:2392	arg1	concentrations					2394:2407	colostral IgG concentrations	2380:2407	colostral IgG concentrations	2380:2407	Increasing litter size tended to be associated with higher protein and lower lactose concentrations in ovine (i.e., singletons vs. twins vs. triplets) and caprine colostrum (i.e., singletons vs. twins), whereas colostral IgG concentrations were not affected by litter size.					
30543881	10	0	theme	mouse	1621:1625	arg1	cells					1645:1649	mouse fibroblast (L929) cells	1621:1649	mouse fibroblast (L929) cells	1621:1649	Furthermore, they were found to be non-toxic by biocompatibility assays on mouse fibroblast (L929) cells.					
30543881	1	1	theme	wound	249:253	arg1	healing					255:261	wound healing	249:261	wound healing process	249:269	Infections, especially those caused by multi-drug resistant pathogens, result in serious problems in wound healing process.					
30543881	4	2	theme	nanofiber	774:782	arg1	CS/PVA/Gel/ZM					790:802	CS/PVA/Gel/ZM	790:802	CS/PVA/Gel/ZM	790:802	The produced nanofiber mats (CS/PVA/Gel/ZM) were chemically crosslinked by glutaraldehyde vapor.					
30543881	4	2	theme	nanofiber	774:782	arg1	mats					784:787	The produced nanofiber mats	761:787	The produced nanofiber mats (CS/PVA/Gel/ZM)	761:803	The produced nanofiber mats (CS/PVA/Gel/ZM) were chemically crosslinked by glutaraldehyde vapor.					
30543881	6	3	theme	AATCC100	1139:1146	arg1	method					1148:1153	the AATCC100 method	1135:1153	the AATCC100 method	1135:1153	The antimicrobial activity of the CS/PVA/Gel/ZM nanofiber mats was determined by the AATCC100 method.					
30543881	6	4	theme	nanofiber	1102:1110	arg1	mats					1112:1115	the CS/PVA/Gel/ZM nanofiber mats	1084:1115	the CS/PVA/Gel/ZM nanofiber mats	1084:1115	The antimicrobial activity of the CS/PVA/Gel/ZM nanofiber mats was determined by the AATCC100 method.					
30543881	3	5	theme	oil	501:503	arg1	oil					501:503	ZM essential oil	488:503	ZM essential oil (0, 2, 5 and 10% (v/v))	488:527	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	5	theme	oil	501:503	arg1	amounts					477:483	Different amounts	467:483	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v))	467:527	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	10	6	from	assays	1611:1616	arg1	cells					1645:1649	mouse fibroblast (L929) cells	1621:1649	mouse fibroblast (L929) cells	1621:1649	Furthermore, they were found to be non-toxic by biocompatibility assays on mouse fibroblast (L929) cells.					
30543881	11	7	theme	wound	1796:1800	arg1	dressings					1802:1810	conventional wound dressings	1783:1810	conventional wound dressings	1783:1810	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	3	8	theme	ZM	488:489	arg1	oil					501:503	ZM essential oil	488:503	ZM essential oil (0, 2, 5 and 10% (v/v))	488:527	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	11	9	theme	nanofiber	1707:1715	arg1	alternatives					1767:1778	promising alternatives	1757:1778	promising alternatives to conventional wound dressings	1757:1810	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	11	9	theme	nanofiber	1707:1715	arg1	mats					1717:1720	CS/PVA/Gel nanofiber mats	1696:1720	CS/PVA/Gel nanofiber mats	1696:1720	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	2	10	theme	multiflora	295:304	arg1	agent					360:364	a strong natural antimicrobial agent	329:364	a strong natural antimicrobial agent	329:364	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	2	10	theme	multiflora	295:304	arg1	oil					321:323	Zataria multiflora (ZM) essential oil	287:323	Zataria multiflora (ZM) essential oil	287:323	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	1	11	theme	resistant	198:206	arg1	pathogens					208:216	multi-drug resistant pathogens	187:216	multi-drug resistant pathogens	187:216	Infections, especially those caused by multi-drug resistant pathogens, result in serious problems in wound healing process.					
30543881	7	12	theme	Candida	1295:1301	arg1	albicans					1303:1310	Candida albicans	1295:1310	Candida albicans	1295:1310	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	3	13	theme	uniform	669:675	arg1	fibers					677:682	uniform fibers	669:682	uniform fibers	669:682	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	0	14	theme	potential	122:130	arg1	dressing					138:145	potential wound dressing	122:145	potential wound dressing	122:145	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	8	15	theme	produced	1378:1385	arg1	nanofibers					1387:1396	the produced nanofibers	1374:1396	the produced nanofibers	1374:1396	Swelling investigations showed that the produced nanofibers have a substantial ability to take up water, in the range of 400-900%.					
30543881	7	16	theme	aeruginosa	1280:1289	arg1	growth					1235:1240	the growth	1231:1240	the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans	1231:1310	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	1	17	from	problems	237:244	arg1	process					263:269	wound healing process	249:269	wound healing process	249:269	Infections, especially those caused by multi-drug resistant pathogens, result in serious problems in wound healing process.					
30543881	5	18	theme	Gas	943:945	arg1	Spectrometry					967:978	Gas Chromatography-Mass Spectrometry	943:978	Gas Chromatography-Mass Spectrometry (GC-MS)	943:986	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	5	18	theme	Gas	943:945	arg1	GC-MS					981:985	GC-MS	981:985	GC-MS	981:985	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	3	19	dep	oil	501:503	arg1	v/v					523:525	v/v	523:525	v/v	523:525	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	19	dep	oil	501:503	arg1	%					520:520	0, 2, 5 and 10%	506:520	0, 2, 5 and 10% (v/v)	506:526	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	2	20	theme	wound	451:455	arg1	dressing					457:464	a novel wound dressing	443:464	a novel wound dressing	443:464	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	8	21	theme	substantial	1405:1415	arg1	ability					1417:1423	a substantial ability	1403:1423	a substantial ability to take up water	1403:1440	Swelling investigations showed that the produced nanofibers have a substantial ability to take up water, in the range of 400-900%.					
30543881	0	22	theme	-based	46:51	arg1	nanofiber					53:61	electrospun poly (vinyl alcohol)-based nanofiber	14:61	electrospun poly (vinyl alcohol)-based nanofiber	14:61	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	2	23	dep	-based	408:413	arg1	alcohol					400:406	vinyl alcohol	394:406	vinyl alcohol	394:406	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	3	24	with	beadless	656:663	arg1	187 ± 40					708:715	187 ± 40	708:715	187 ± 40	708:715	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	24	with	beadless	656:663	arg1	154 ± 27					698:705	154 ± 27	698:705	154 ± 27	698:705	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	24	with	beadless	656:663	arg1	95 ± 14					689:695	95 ± 14	689:695	95 ± 14	689:695	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	24	with	beadless	656:663	arg1	218 ± 58 nm					721:731	218 ± 58 nm	721:731	218 ± 58 nm	721:731	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	11	25	theme	obtained	1656:1663	arg1	results					1665:1671	The obtained results	1652:1671	The obtained results	1652:1671	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	5	26	theme	ZM	887:888	arg1	oil					900:902	ZM essential oil	887:902	ZM essential oil	887:902	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	2	27	theme	antimicrobial	346:358	arg1	agent					360:364	a strong natural antimicrobial agent	329:364	a strong natural antimicrobial agent	329:364	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	2	27	theme	antimicrobial	346:358	arg1	oil					321:323	Zataria multiflora (ZM) essential oil	287:323	Zataria multiflora (ZM) essential oil	287:323	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	0	28	dep	mats	63:66	arg1	incorporated					68:79	incorporated	68:79	mats incorporated with Zataria multiflora essential oil as potential wound dressing	63:145	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	2	29	theme	vinyl	394:398	arg1	alcohol					400:406	vinyl alcohol	394:406	vinyl alcohol	394:406	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	5	30	theme	mats	918:921	arg1	compositions					871:882	The chemical compositions	858:882	The chemical compositions of ZM essential oil and nanofiber mats	858:921	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	0	31	dep	-based	46:51	arg1	alcohol					38:44	vinyl alcohol	32:44	vinyl alcohol	32:44	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	8	32	theme	%	1466:1466	arg1	range					1450:1454	the range	1446:1454	the range of 400-900%	1446:1466	Swelling investigations showed that the produced nanofibers have a substantial ability to take up water, in the range of 400-900%.					
30543881	9	33	theme	mats	1508:1511	arg1	properties					1480:1489	Mechanical properties	1469:1489	Mechanical properties of the nanofiber mats	1469:1511	Mechanical properties of the nanofiber mats were studied by tensile testing.					
30543881	7	34	theme	essential	1196:1204	arg1	oil					1206:1208	ZM essential oil	1193:1208	ZM essential oil	1193:1208	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	2	35	theme	nanofiber	415:423	arg1	mats					425:428	poly (vinyl alcohol)-based nanofiber mats	388:428	poly (vinyl alcohol)-based nanofiber mats	388:428	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	6	36	theme	mats	1112:1115	arg1	activity					1072:1079	The antimicrobial activity	1054:1079	The antimicrobial activity of the CS/PVA/Gel/ZM nanofiber mats	1054:1115	The antimicrobial activity of the CS/PVA/Gel/ZM nanofiber mats was determined by the AATCC100 method.					
30543881	3	37	theme	alcohol	572:578	arg1	CS/PVA/Gel					590:599	CS/PVA/Gel	590:599	CS/PVA/Gel	590:599	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	37	theme	alcohol	572:578	arg1	/gelatin					580:587	chitosan/poly(vinyl alcohol)/gelatin	552:587	chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions	552:610	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	8	38	theme	Swelling	1338:1345	arg1	investigations					1347:1360	Swelling investigations	1338:1360	Swelling investigations	1338:1360	Swelling investigations showed that the produced nanofibers have a substantial ability to take up water, in the range of 400-900%.					
30543881	10	39	theme	biocompatibility	1594:1609	arg1	assays					1611:1616	biocompatibility assays	1594:1616	biocompatibility assays on mouse fibroblast (L929) cells	1594:1649	Furthermore, they were found to be non-toxic by biocompatibility assays on mouse fibroblast (L929) cells.					
30543881	9	40	theme	tensile	1529:1535	arg1	testing					1537:1543	tensile testing	1529:1543	tensile testing	1529:1543	Mechanical properties of the nanofiber mats were studied by tensile testing.					
30543881	8	41	contain	have	1398:1401	arg2	ability					1417:1423	a substantial ability	1403:1423	a substantial ability to take up water	1403:1440	Swelling investigations showed that the produced nanofibers have a substantial ability to take up water, in the range of 400-900%.					
30543881	8	41	contain	have	1398:1401	arg1	nanofibers					1387:1396	the produced nanofibers	1374:1396	the produced nanofibers	1374:1396	Swelling investigations showed that the produced nanofibers have a substantial ability to take up water, in the range of 400-900%.					
30543881	5	42	dep	Fourier	992:998	arg1	Transform					1000:1008	Transform	1000:1008	Transform Infrared Spectroscopy (FTIR)	1000:1037	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	11	43	theme	promising	1757:1765	arg1	alternatives					1767:1778	promising alternatives	1757:1778	promising alternatives to conventional wound dressings	1757:1810	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	11	43	theme	promising	1757:1765	arg1	mats					1717:1720	CS/PVA/Gel nanofiber mats	1696:1720	CS/PVA/Gel nanofiber mats	1696:1720	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	3	44	theme	Different	467:475	arg1	oil					501:503	ZM essential oil	488:503	ZM essential oil (0, 2, 5 and 10% (v/v))	488:527	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	44	theme	Different	467:475	arg1	amounts					477:483	Different amounts	467:483	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v))	467:527	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	1	45	theme	serious	229:235	arg1	problems					237:244	serious problems	229:244	serious problems in wound healing process	229:269	Infections, especially those caused by multi-drug resistant pathogens, result in serious problems in wound healing process.					
30543881	5	46	theme	nanofiber	908:916	arg1	mats					918:921	nanofiber mats	908:921	nanofiber mats	908:921	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	10	47	theme	fibroblast	1627:1636	arg1	cells					1645:1649	mouse fibroblast (L929) cells	1621:1649	mouse fibroblast (L929) cells	1621:1649	Furthermore, they were found to be non-toxic by biocompatibility assays on mouse fibroblast (L929) cells.					
30543881	7	48	theme	nanofiber	1160:1168	arg1	mat					1170:1172	The nanofiber mat	1156:1172	The nanofiber mat loaded with 10% of ZM essential oil	1156:1208	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	3	49	theme	/gelatin	580:587	arg1	solutions					602:610	chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions	552:610	chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions	552:610	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	50	theme	essential	491:499	arg1	oil					501:503	ZM essential oil	488:503	ZM essential oil (0, 2, 5 and 10% (v/v))	488:527	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	1	51	theme	healing	255:261	arg1	process					263:269	wound healing process	249:269	wound healing process	249:269	Infections, especially those caused by multi-drug resistant pathogens, result in serious problems in wound healing process.					
30543881	4	52	theme	produced	765:772	arg1	CS/PVA/Gel/ZM					790:802	CS/PVA/Gel/ZM	790:802	CS/PVA/Gel/ZM	790:802	The produced nanofiber mats (CS/PVA/Gel/ZM) were chemically crosslinked by glutaraldehyde vapor.					
30543881	4	52	theme	produced	765:772	arg1	mats					784:787	The produced nanofiber mats	761:787	The produced nanofiber mats (CS/PVA/Gel/ZM)	761:803	The produced nanofiber mats (CS/PVA/Gel/ZM) were chemically crosslinked by glutaraldehyde vapor.					
30543881	6	53	theme	CS/PVA/Gel/ZM	1088:1100	arg1	mats					1112:1115	the CS/PVA/Gel/ZM nanofiber mats	1084:1115	the CS/PVA/Gel/ZM nanofiber mats	1084:1115	The antimicrobial activity of the CS/PVA/Gel/ZM nanofiber mats was determined by the AATCC100 method.					
30543881	6	54	theme	antimicrobial	1058:1070	arg1	activity					1072:1079	The antimicrobial activity	1054:1079	The antimicrobial activity of the CS/PVA/Gel/ZM nanofiber mats	1054:1115	The antimicrobial activity of the CS/PVA/Gel/ZM nanofiber mats was determined by the AATCC100 method.					
30543881	2	55	theme	Zataria	287:293	arg1	agent					360:364	a strong natural antimicrobial agent	329:364	a strong natural antimicrobial agent	329:364	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	2	55	theme	Zataria	287:293	arg1	oil					321:323	Zataria multiflora (ZM) essential oil	287:323	Zataria multiflora (ZM) essential oil	287:323	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	1	56	theme	multi-drug	187:196	arg1	pathogens					208:216	multi-drug resistant pathogens	187:216	multi-drug resistant pathogens	187:216	Infections, especially those caused by multi-drug resistant pathogens, result in serious problems in wound healing process.					
30543881	7	57	theme	incubation	1326:1335	arg1	24 h					1318:1321	24 h	1318:1321	24 h of incubation	1318:1335	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	2	58	theme	strong	331:336	arg1	agent					360:364	a strong natural antimicrobial agent	329:364	a strong natural antimicrobial agent	329:364	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	2	58	theme	strong	331:336	arg1	oil					321:323	Zataria multiflora (ZM) essential oil	287:323	Zataria multiflora (ZM) essential oil	287:323	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	11	59	theme	CS/PVA/Gel	1696:1705	arg1	alternatives					1767:1778	promising alternatives	1757:1778	promising alternatives to conventional wound dressings	1757:1810	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	11	59	theme	CS/PVA/Gel	1696:1705	arg1	mats					1717:1720	CS/PVA/Gel nanofiber mats	1696:1720	CS/PVA/Gel nanofiber mats	1696:1720	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	0	60	theme	wound	132:136	arg1	dressing					138:145	potential wound dressing	122:145	potential wound dressing	122:145	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	7	61	theme	albicans	1303:1310	arg1	growth					1235:1240	the growth	1231:1240	the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans	1231:1310	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	11	62	theme	ZM	1735:1736	arg1	oil					1748:1750	ZM essential oil	1735:1750	ZM essential oil	1735:1750	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	5	63	theme	Chromatography-Mass	947:965	arg1	Spectrometry					967:978	Gas Chromatography-Mass Spectrometry	943:978	Gas Chromatography-Mass Spectrometry (GC-MS)	943:986	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	5	63	theme	Chromatography-Mass	947:965	arg1	GC-MS					981:985	GC-MS	981:985	GC-MS	981:985	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	11	64	theme	essential	1738:1746	arg1	oil					1748:1750	ZM essential oil	1735:1750	ZM essential oil	1735:1750	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30543881	0	65	theme	nanofiber	53:61	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber	0:61	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	2	66	theme	novel	445:449	arg1	dressing					457:464	a novel wound dressing	443:464	a novel wound dressing	443:464	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	7	67	theme	aureus	1260:1265	arg1	growth					1235:1240	the growth	1231:1240	the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans	1231:1310	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	0	68	theme	Zataria	86:92	arg1	oil					115:117	Zataria multiflora essential oil	86:117	Zataria multiflora essential oil	86:117	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	5	69	theme	oil	900:902	arg1	compositions					871:882	The chemical compositions	858:882	The chemical compositions of ZM essential oil and nanofiber mats	858:921	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	5	70	theme	chemical	862:869	arg1	compositions					871:882	The chemical compositions	858:882	The chemical compositions of ZM essential oil and nanofiber mats	858:921	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	4	71	theme	glutaraldehyde	836:849	arg1	vapor					851:855	glutaraldehyde vapor	836:855	glutaraldehyde vapor	836:855	The produced nanofiber mats (CS/PVA/Gel/ZM) were chemically crosslinked by glutaraldehyde vapor.					
30543881	5	72	theme	Infrared	1010:1017	arg1	Spectroscopy					1019:1030	Infrared Spectroscopy	1010:1030	Infrared Spectroscopy (FTIR)	1010:1037	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	5	72	theme	Infrared	1010:1017	arg1	FTIR					1033:1036	FTIR	1033:1036	FTIR	1033:1036	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	3	73	with	fibers	677:682	arg1	187 ± 40					708:715	187 ± 40	708:715	187 ± 40	708:715	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	73	with	fibers	677:682	arg1	154 ± 27					698:705	154 ± 27	698:705	154 ± 27	698:705	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	73	with	fibers	677:682	arg1	95 ± 14					689:695	95 ± 14	689:695	95 ± 14	689:695	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	73	with	fibers	677:682	arg1	218 ± 58 nm					721:731	218 ± 58 nm	721:731	218 ± 58 nm	721:731	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	0	74	dep	Zataria	86:92	arg1	multiflora					94:103	multiflora	94:103	multiflora	94:103	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	0	74	dep	Zataria	86:92	arg1	essential					105:113	essential	105:113	essential	105:113	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	9	75	theme	Mechanical	1469:1478	arg1	properties					1480:1489	Mechanical properties	1469:1489	Mechanical properties of the nanofiber mats	1469:1511	Mechanical properties of the nanofiber mats were studied by tensile testing.					
30543881	2	76	theme	natural	338:344	arg1	agent					360:364	a strong natural antimicrobial agent	329:364	a strong natural antimicrobial agent	329:364	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	2	76	theme	natural	338:344	arg1	oil					321:323	Zataria multiflora (ZM) essential oil	287:323	Zataria multiflora (ZM) essential oil	287:323	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	7	77	theme	oil	1206:1208	arg1	oil					1206:1208	ZM essential oil	1193:1208	ZM essential oil	1193:1208	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	7	77	theme	oil	1206:1208	arg1	%					1188:1188	10%	1186:1188	10% of ZM essential oil	1186:1208	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	3	78	from	187 ± 40	708:715	arg1	diameters					736:744	diameters	736:744	diameters	736:744	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	10	79	theme	L929	1639:1642	arg1	cells					1645:1649	mouse fibroblast (L929) cells	1621:1649	mouse fibroblast (L929) cells	1621:1649	Furthermore, they were found to be non-toxic by biocompatibility assays on mouse fibroblast (L929) cells.					
30543881	0	80	theme	vinyl	32:36	arg1	alcohol					38:44	vinyl alcohol	32:44	vinyl alcohol	32:44	Evaluation of electrospun poly (vinyl alcohol)-based nanofiber mats incorporated with Zataria multiflora essential oil as potential wound dressing.					
30543881	9	81	theme	nanofiber	1498:1506	arg1	mats					1508:1511	the nanofiber mats	1494:1511	the nanofiber mats	1494:1511	Mechanical properties of the nanofiber mats were studied by tensile testing.					
30543881	7	82	theme	ZM	1193:1194	arg1	oil					1206:1208	ZM essential oil	1193:1208	ZM essential oil	1193:1208	The nanofiber mat loaded with 10% of ZM essential oil completely inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans after 24 h of incubation.					
30543881	3	83	from	218 ± 58 nm	721:731	arg1	diameters					736:744	diameters	736:744	diameters	736:744	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	2	84	theme	-based	408:413	arg1	mats					425:428	poly (vinyl alcohol)-based nanofiber mats	388:428	poly (vinyl alcohol)-based nanofiber mats	388:428	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	3	85	from	95 ± 14	689:695	arg1	diameters					736:744	diameters	736:744	diameters	736:744	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	86	theme	vinyl	566:570	arg1	CS/PVA/Gel					590:599	CS/PVA/Gel	590:599	CS/PVA/Gel	590:599	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	86	theme	vinyl	566:570	arg1	/gelatin					580:587	chitosan/poly(vinyl alcohol)/gelatin	552:587	chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions	552:610	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	2	87	theme	essential	311:319	arg1	agent					360:364	a strong natural antimicrobial agent	329:364	a strong natural antimicrobial agent	329:364	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	2	87	theme	essential	311:319	arg1	oil					321:323	Zataria multiflora (ZM) essential oil	287:323	Zataria multiflora (ZM) essential oil	287:323	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	5	88	theme	essential	890:898	arg1	oil					900:902	ZM essential oil	887:902	ZM essential oil	887:902	The chemical compositions of ZM essential oil and nanofiber mats were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR), respectively.					
30543881	3	89	from	154 ± 27	698:705	arg1	diameters					736:744	diameters	736:744	diameters	736:744	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	90	theme	chitosan/poly	552:564	arg1	CS/PVA/Gel					590:599	CS/PVA/Gel	590:599	CS/PVA/Gel	590:599	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	3	90	theme	chitosan/poly	552:564	arg1	/gelatin					580:587	chitosan/poly(vinyl alcohol)/gelatin	552:587	chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions	552:610	Different amounts of ZM essential oil (0, 2, 5 and 10% (v/v)) were incorporated into chitosan/poly(vinyl alcohol)/gelatin (CS/PVA/Gel) solutions and then were successfully electrospun into beadless and uniform fibers with 95 ± 14, 154 ± 27, 187 ± 40 and 218 ± 58 nm in diameters, respectively.					
30543881	2	91	theme	ZM	307:308	arg1	agent					360:364	a strong natural antimicrobial agent	329:364	a strong natural antimicrobial agent	329:364	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	2	91	theme	ZM	307:308	arg1	oil					321:323	Zataria multiflora (ZM) essential oil	287:323	Zataria multiflora (ZM) essential oil	287:323	In this study, Zataria multiflora (ZM) essential oil, as a strong natural antimicrobial agent, is incorporated into poly (vinyl alcohol)-based nanofiber mats to fabricate a novel wound dressing.					
30543881	11	92	theme	conventional	1783:1794	arg1	dressings					1802:1810	conventional wound dressings	1783:1810	conventional wound dressings	1783:1810	The obtained results have demonstrated that CS/PVA/Gel nanofiber mats, loaded with ZM essential oil, are promising alternatives to conventional wound dressings.					
30082640	7	0	theme	dimensional	1286:1296	arg1	stability					1298:1306	good dimensional stability	1281:1306	good dimensional stability	1281:1306	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	7	1	from	cytocompatibility	1231:1247	arg1	fluid					1320:1324	synovial fluid	1311:1324	synovial fluid	1311:1324	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	7	2	theme	satisfactory	1210:1221	arg1	cytocompatibility					1231:1247	satisfactory ex vivo cytocompatibility;	1210:1248	satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid	1210:1324	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	5	3	theme	systemic	958:965	arg1	route					992:996	both systemic and local administration route	953:996	both systemic and local administration route	953:996	Dexamethasone (DXM) is a glucorticoid drug commonly used in joint disease treatment for both systemic and local administration route.					
30082640	6	4	theme	conjugate-based	1066:1080	arg1	NPs					1082:1084	GE11-PLGA conjugate-based NPs	1056:1084	GE11-PLGA conjugate-based NPs	1056:1084	In the present research; DXM was efficiently loaded into GE11-PLGA conjugate-based NPs through an eco-friendly nanoprecipitation method set up for this purpose.					
30082640	8	5	from	temperature	1578:1588	arg1	state					1555:1559	gel state	1551:1559	gel state at physiological temperature	1551:1588	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	7	6	theme	ex	1223:1224	arg1	cytocompatibility					1231:1247	satisfactory ex vivo cytocompatibility;	1210:1248	satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid	1210:1324	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	1	7	theme	promising	198:206	arg1	option					234:239	promising intravenously therapeutic option	198:239	promising intravenously therapeutic option for most chronic joint diseases	198:271	Selectively targeted nanoscale drug delivery systems have recently emerged as promising intravenously therapeutic option for most chronic joint diseases.					
30082640	6	8	theme	GE11-PLGA	1056:1064	arg1	NPs					1082:1084	GE11-PLGA conjugate-based NPs	1056:1084	GE11-PLGA conjugate-based NPs	1056:1084	In the present research; DXM was efficiently loaded into GE11-PLGA conjugate-based NPs through an eco-friendly nanoprecipitation method set up for this purpose.					
30082640	2	9	theme	smart	389:393	arg1	nanoparticles					395:407	smart nanoparticles	389:407	smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR)	389:527	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	2	9	theme	smart	389:393	arg1	NPs					410:412	NPs	410:412	NPs	410:412	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	9	10	theme	local	1677:1681	arg1	release					1683:1689	the local release	1673:1689	the local release of active agent	1673:1705	Moreover; the use of thermosetting chitosan-based hydrogel extends the local release of active agent; DXM.					
30082640	8	11	theme	able	1507:1510	arg1	hydrogel					1498:1505	a biocompatible composite hydrogel	1472:1505	a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature	1472:1588	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	7	12	theme	good	1281:1284	arg1	stability					1298:1306	good dimensional stability	1281:1306	good dimensional stability	1281:1306	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	7	13	with	cytocompatibility	1231:1247	arg1	stability					1298:1306	good dimensional stability	1281:1306	good dimensional stability	1281:1306	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	7	13	with	cytocompatibility	1231:1247	arg1	nm					1273:1274	≤150 nm	1268:1274	≤150 nm	1268:1274	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	7	13	with	cytocompatibility	1231:1247	arg1	size					1262:1265	proper size	1255:1265	proper size (≤150 nm)	1255:1275	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	4	14	theme	local	832:836	arg1	effects					856:862	local and systemic side effects	832:862	local and systemic side effects	832:862	The selective uptake could help to tune drug effectiveness in joints and to decrease local and systemic side effects.					
30082640	3	15	theme	GE11-PLGA	530:538	arg1	NPs					556:558	GE11-PLGA conjugate-based NPs	530:558	GE11-PLGA conjugate-based NPs	530:558	GE11-PLGA conjugate-based NPs are specifically uptaken by EGFR-overexpressed fibroblast; such as synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation.					
30082640	3	16	theme	conjugate-based	540:554	arg1	NPs					556:558	GE11-PLGA conjugate-based NPs	530:558	GE11-PLGA conjugate-based NPs	530:558	GE11-PLGA conjugate-based NPs are specifically uptaken by EGFR-overexpressed fibroblast; such as synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation.					
30082640	7	17	theme	GE11-PLGA	1171:1179	arg1	NPs					1197:1199	DXM loaded GE11-PLGA conjugate-based NPs	1160:1199	DXM loaded GE11-PLGA conjugate-based NPs	1160:1199	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	5	18	theme	joint	925:929	arg1	treatment					939:947	joint disease treatment	925:947	joint disease treatment	925:947	Dexamethasone (DXM) is a glucorticoid drug commonly used in joint disease treatment for both systemic and local administration route.					
30082640	3	19	theme	EGFR-overexpressed	588:605	arg1	fibroblast					607:616	EGFR-overexpressed fibroblast;	588:617	EGFR-overexpressed fibroblast; such as synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation	588:744	GE11-PLGA conjugate-based NPs are specifically uptaken by EGFR-overexpressed fibroblast; such as synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation.					
30082640	5	20	theme	disease	931:937	arg1	treatment					939:947	joint disease treatment	925:947	joint disease treatment	925:947	Dexamethasone (DXM) is a glucorticoid drug commonly used in joint disease treatment for both systemic and local administration route.					
30082640	2	21	theme	intra-articular	428:442	arg1	administration					444:457	intra-articular administration	428:457	intra-articular administration	428:457	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	7	22	theme	conjugate-based	1181:1195	arg1	NPs					1197:1199	DXM loaded GE11-PLGA conjugate-based NPs	1160:1199	DXM loaded GE11-PLGA conjugate-based NPs	1160:1199	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	8	23	theme	GE11-PLGA	1390:1398	arg1	NPs					1416:1418	DXM loaded GE11-PLGA conjugate-based NPs	1379:1418	DXM loaded GE11-PLGA conjugate-based NPs	1379:1418	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	0	24	theme	Intra-Articular	0:14	arg1	Formulation					16:26	Intra-Articular Formulation	0:26	Intra-Articular Formulation of GE11-PLGA Conjugate-Based	0:55	Intra-Articular Formulation of GE11-PLGA Conjugate-Based NPs for Dexamethasone Selective Targeting-In Vitro Evaluation.					
30082640	1	25	theme	therapeutic	222:232	arg1	option					234:239	promising intravenously therapeutic option	198:239	promising intravenously therapeutic option for most chronic joint diseases	198:271	Selectively targeted nanoscale drug delivery systems have recently emerged as promising intravenously therapeutic option for most chronic joint diseases.					
30082640	2	26	theme	Factor	506:511	arg1	EGFR					523:526	EGFR	523:526	EGFR	523:526	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	2	26	theme	Factor	506:511	arg1	Receptor					513:520	Epidermal Growth Factor Receptor	489:520	Epidermal Growth Factor Receptor (EGFR)	489:527	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	9	27	theme	chitosan-based	1641:1654	arg1	hydrogel					1656:1663	thermosetting chitosan-based hydrogel	1627:1663	thermosetting chitosan-based hydrogel	1627:1663	Moreover; the use of thermosetting chitosan-based hydrogel extends the local release of active agent; DXM.					
30082640	2	28	used	used	373:376	arg2	conjugate					359:367	a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate	280:367	a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate	280:367	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	4	29	theme	selective	751:759	arg1	uptake					761:766	The selective uptake	747:766	The selective uptake	747:766	The selective uptake could help to tune drug effectiveness in joints and to decrease local and systemic side effects.					
30082640	2	30	theme	Growth	499:504	arg1	EGFR					523:526	EGFR	523:526	EGFR	523:526	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	2	30	theme	Growth	499:504	arg1	Receptor					513:520	Epidermal Growth Factor Receptor	489:520	Epidermal Growth Factor Receptor (EGFR)	489:527	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	7	31	theme	loaded	1164:1169	arg1	NPs					1197:1199	DXM loaded GE11-PLGA conjugate-based NPs	1160:1199	DXM loaded GE11-PLGA conjugate-based NPs	1160:1199	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	2	32	theme	-based	352:357	arg1	conjugate					359:367	a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate	280:367	a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate	280:367	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	8	33	theme	biocompatible	1474:1486	arg1	hydrogel					1498:1505	a biocompatible composite hydrogel	1472:1505	a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature	1472:1588	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	8	34	theme	Intra-articular	1327:1341	arg1	formulation					1343:1353	Intra-articular formulation	1327:1353	Intra-articular formulation	1327:1353	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	0	35	theme	GE11-PLGA	31:39	arg1	Conjugate-Based					41:55	GE11-PLGA Conjugate-Based	31:55	GE11-PLGA Conjugate-Based	31:55	Intra-Articular Formulation of GE11-PLGA Conjugate-Based NPs for Dexamethasone Selective Targeting-In Vitro Evaluation.					
30082640	8	36	theme	conjugate-based	1400:1414	arg1	NPs					1416:1418	DXM loaded GE11-PLGA conjugate-based NPs	1379:1418	DXM loaded GE11-PLGA conjugate-based NPs	1379:1418	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	5	37	theme	local	971:975	arg1	route					992:996	both systemic and local administration route	953:996	both systemic and local administration route	953:996	Dexamethasone (DXM) is a glucorticoid drug commonly used in joint disease treatment for both systemic and local administration route.					
30082640	1	38	theme	targeted	132:139	arg1	systems					165:171	Selectively targeted nanoscale drug delivery systems	120:171	Selectively targeted nanoscale drug delivery systems	120:171	Selectively targeted nanoscale drug delivery systems have recently emerged as promising intravenously therapeutic option for most chronic joint diseases.					
30082640	1	39	theme	most	245:248	arg1	diseases					264:271	most chronic joint diseases	245:271	most chronic joint diseases	245:271	Selectively targeted nanoscale drug delivery systems have recently emerged as promising intravenously therapeutic option for most chronic joint diseases.					
30082640	7	40	dep	ex	1223:1224	arg1	vivo					1226:1229	vivo	1226:1229	vivo	1226:1229	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	4	41	theme	side	851:854	arg1	effects					856:862	local and systemic side effects	832:862	local and systemic side effects	832:862	The selective uptake could help to tune drug effectiveness in joints and to decrease local and systemic side effects.					
30082640	3	42	theme	destructive	715:725	arg1	inflammation					733:744	destructive joint inflammation	715:744	destructive joint inflammation	715:744	GE11-PLGA conjugate-based NPs are specifically uptaken by EGFR-overexpressed fibroblast; such as synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation.					
30082640	1	43	theme	nanoscale	141:149	arg1	systems					165:171	Selectively targeted nanoscale drug delivery systems	120:171	Selectively targeted nanoscale drug delivery systems	120:171	Selectively targeted nanoscale drug delivery systems have recently emerged as promising intravenously therapeutic option for most chronic joint diseases.					
30082640	9	44	theme	hydrogel	1656:1663	arg1	use					1620:1622	the use	1616:1622	the use of thermosetting chitosan-based hydrogel	1616:1663	Moreover; the use of thermosetting chitosan-based hydrogel extends the local release of active agent; DXM.					
30082640	1	45	theme	chronic	250:256	arg1	diseases					264:271	most chronic joint diseases	245:271	most chronic joint diseases	245:271	Selectively targeted nanoscale drug delivery systems have recently emerged as promising intravenously therapeutic option for most chronic joint diseases.					
30082640	5	46	theme	administration	977:990	arg1	route					992:996	both systemic and local administration route	953:996	both systemic and local administration route	953:996	Dexamethasone (DXM) is a glucorticoid drug commonly used in joint disease treatment for both systemic and local administration route.					
30082640	9	47	theme	agent	1701:1705	arg1	release					1683:1689	the local release	1673:1689	the local release of active agent	1673:1705	Moreover; the use of thermosetting chitosan-based hydrogel extends the local release of active agent; DXM.					
30082640	3	48	theme	joint	727:731	arg1	inflammation					733:744	destructive joint inflammation	715:744	destructive joint inflammation	715:744	GE11-PLGA conjugate-based NPs are specifically uptaken by EGFR-overexpressed fibroblast; such as synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation.					
30082640	8	49	dep	loaded	1383:1388	arg1	DXM					1379:1381	DXM	1379:1381	DXM	1379:1381	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	8	50	from	state	1540:1544	arg1	turn					1523:1526	turn	1523:1526	turn	1523:1526	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	1	51	theme	joint	258:262	arg1	diseases					264:271	most chronic joint diseases	245:271	most chronic joint diseases	245:271	Selectively targeted nanoscale drug delivery systems have recently emerged as promising intravenously therapeutic option for most chronic joint diseases.					
30082640	0	52	theme	Conjugate-Based	41:55	arg1	Formulation					16:26	Intra-Articular Formulation	0:26	Intra-Articular Formulation of GE11-PLGA Conjugate-Based	0:55	Intra-Articular Formulation of GE11-PLGA Conjugate-Based NPs for Dexamethasone Selective Targeting-In Vitro Evaluation.					
30082640	7	53	dep	loaded	1164:1169	arg1	DXM					1160:1162	DXM	1160:1162	DXM	1160:1162	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	2	54	theme	Epidermal	489:497	arg1	EGFR					523:526	EGFR	523:526	EGFR	523:526	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	2	54	theme	Epidermal	489:497	arg1	Receptor					513:520	Epidermal Growth Factor Receptor	489:520	Epidermal Growth Factor Receptor (EGFR)	489:527	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	8	55	theme	physiological	1564:1576	arg1	temperature					1578:1588	physiological temperature	1564:1588	physiological temperature	1564:1588	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	6	56	theme	nanoprecipitation	1110:1126	arg1	method					1128:1133	an eco-friendly nanoprecipitation method	1094:1133	an eco-friendly nanoprecipitation method set up for this purpose	1094:1157	In the present research; DXM was efficiently loaded into GE11-PLGA conjugate-based NPs through an eco-friendly nanoprecipitation method set up for this purpose.					
30082640	0	57	theme	Dexamethasone	65:77	arg1	Evaluation					108:117	Dexamethasone Selective Targeting-In Vitro Evaluation	65:117	Dexamethasone Selective Targeting-In Vitro Evaluation	65:117	Intra-Articular Formulation of GE11-PLGA Conjugate-Based NPs for Dexamethasone Selective Targeting-In Vitro Evaluation.					
30082640	6	58	theme	present	1006:1012	arg1	research					1014:1021	the present research	1002:1021	the present research	1002:1021	In the present research; DXM was efficiently loaded into GE11-PLGA conjugate-based NPs through an eco-friendly nanoprecipitation method set up for this purpose.					
30082640	7	59	theme	synovial	1311:1318	arg1	fluid					1320:1324	synovial fluid	1311:1324	synovial fluid	1311:1324	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	8	60	theme	liquid	1533:1538	arg1	state					1540:1544	liquid state	1533:1544	liquid state	1533:1544	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	8	61	theme	composite	1488:1496	arg1	hydrogel					1498:1505	a biocompatible composite hydrogel	1472:1505	a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature	1472:1588	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	1	62	theme	drug	151:154	arg1	systems					165:171	Selectively targeted nanoscale drug delivery systems	120:171	Selectively targeted nanoscale drug delivery systems	120:171	Selectively targeted nanoscale drug delivery systems have recently emerged as promising intravenously therapeutic option for most chronic joint diseases.					
30082640	8	63	theme	chitosan-based	1439:1452	arg1	hydrogel					1454:1461	chitosan-based hydrogel	1439:1461	chitosan-based hydrogel	1439:1461	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	1	64	theme	delivery	156:163	arg1	systems					165:171	Selectively targeted nanoscale drug delivery systems	120:171	Selectively targeted nanoscale drug delivery systems	120:171	Selectively targeted nanoscale drug delivery systems have recently emerged as promising intravenously therapeutic option for most chronic joint diseases.					
30082640	7	65	theme	proper	1255:1260	arg1	size					1262:1265	proper size	1255:1265	proper size (≤150 nm)	1255:1275	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	7	65	theme	proper	1255:1260	arg1	nm					1273:1274	≤150 nm	1268:1274	≤150 nm	1268:1274	DXM loaded GE11-PLGA conjugate-based NPs revealed satisfactory ex vivo cytocompatibility; with proper size (≤150 nm) and good dimensional stability in synovial fluid.					
30082640	4	66	theme	drug	787:790	arg1	effectiveness					792:804	drug effectiveness	787:804	drug effectiveness in joints	787:814	The selective uptake could help to tune drug effectiveness in joints and to decrease local and systemic side effects.					
30082640	3	67	theme	cellular	665:672	arg1	synoviocytes					627:638	synoviocytes;	627:639	synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation	627:744	GE11-PLGA conjugate-based NPs are specifically uptaken by EGFR-overexpressed fibroblast; such as synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation.					
30082640	3	67	theme	cellular	665:672	arg1	component					674:682	the primarily cellular component	651:682	the primarily cellular component involved in the development of destructive joint inflammation	651:744	GE11-PLGA conjugate-based NPs are specifically uptaken by EGFR-overexpressed fibroblast; such as synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation.					
30082640	9	68	theme	active	1694:1699	arg1	agent					1701:1705	active agent	1694:1705	active agent	1694:1705	Moreover; the use of thermosetting chitosan-based hydrogel extends the local release of active agent; DXM.					
30082640	0	69	dep	Targeting-In	89:100	arg1	Vitro					102:106	Vitro	102:106	Vitro	102:106	Intra-Articular Formulation of GE11-PLGA Conjugate-Based NPs for Dexamethasone Selective Targeting-In Vitro Evaluation.					
30082640	6	70	theme	eco-friendly	1097:1108	arg1	method					1128:1133	an eco-friendly nanoprecipitation method	1094:1133	an eco-friendly nanoprecipitation method set up for this purpose	1094:1157	In the present research; DXM was efficiently loaded into GE11-PLGA conjugate-based NPs through an eco-friendly nanoprecipitation method set up for this purpose.					
30082640	4	71	theme	systemic	842:849	arg1	effects					856:862	local and systemic side effects	832:862	local and systemic side effects	832:862	The selective uptake could help to tune drug effectiveness in joints and to decrease local and systemic side effects.					
30082640	3	72	theme	inflammation	733:744	arg1	development					700:710	the development	696:710	the development of destructive joint inflammation	696:744	GE11-PLGA conjugate-based NPs are specifically uptaken by EGFR-overexpressed fibroblast; such as synoviocytes; which are the primarily cellular component involved in the development of destructive joint inflammation.					
30082640	2	73	dep	-based	352:357	arg1	synthetized					288:298	synthetized	288:298	synthetized	288:298	Here, a newly synthetized dodecapeptide (GE11)-polylactide-co-glycolide (PLGA)-based conjugate was used to prepare smart nanoparticles (NPs) intended for intra-articular administration and for selectively targeting Epidermal Growth Factor Receptor (EGFR).					
30082640	9	74	dep	extends	1665:1671	arg1	DXM					1708:1710	DXM	1708:1710	DXM	1708:1710	Moreover; the use of thermosetting chitosan-based hydrogel extends the local release of active agent; DXM.					
30082640	0	75	theme	Targeting-In	89:100	arg1	Evaluation					108:117	Dexamethasone Selective Targeting-In Vitro Evaluation	65:117	Dexamethasone Selective Targeting-In Vitro Evaluation	65:117	Intra-Articular Formulation of GE11-PLGA Conjugate-Based NPs for Dexamethasone Selective Targeting-In Vitro Evaluation.					
30082640	5	76	theme	glucorticoid	890:901	arg1	Dexamethasone					865:877	Dexamethasone	865:877	Dexamethasone (DXM)	865:883	Dexamethasone (DXM) is a glucorticoid drug commonly used in joint disease treatment for both systemic and local administration route.					
30082640	5	76	theme	glucorticoid	890:901	arg1	drug					903:906	a glucorticoid drug	888:906	a glucorticoid drug commonly used in joint disease treatment for both systemic and local administration route	888:996	Dexamethasone (DXM) is a glucorticoid drug commonly used in joint disease treatment for both systemic and local administration route.					
30082640	4	77	from	effectiveness	792:804	arg1	joints					809:814	joints	809:814	joints	809:814	The selective uptake could help to tune drug effectiveness in joints and to decrease local and systemic side effects.					
30082640	0	78	theme	Selective	79:87	arg1	Evaluation					108:117	Dexamethasone Selective Targeting-In Vitro Evaluation	65:117	Dexamethasone Selective Targeting-In Vitro Evaluation	65:117	Intra-Articular Formulation of GE11-PLGA Conjugate-Based NPs for Dexamethasone Selective Targeting-In Vitro Evaluation.					
30082640	8	79	theme	gel	1551:1553	arg1	state					1555:1559	gel state	1551:1559	gel state at physiological temperature	1551:1588	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
30082640	9	80	theme	thermosetting	1627:1639	arg1	hydrogel					1656:1663	thermosetting chitosan-based hydrogel	1627:1663	thermosetting chitosan-based hydrogel	1627:1663	Moreover; the use of thermosetting chitosan-based hydrogel extends the local release of active agent; DXM.					
30082640	8	81	theme	loaded	1383:1388	arg1	NPs					1416:1418	DXM loaded GE11-PLGA conjugate-based NPs	1379:1418	DXM loaded GE11-PLGA conjugate-based NPs	1379:1418	Intra-articular formulation was developed embedding DXM loaded GE11-PLGA conjugate-based NPs into thermosetting chitosan-based hydrogel; forming a biocompatible composite hydrogel able to quickly turn from liquid state into gel state at physiological temperature; within 15 min.					
29584939	5	0	theme	early	666:670	arg1	apoptosis					699:707	early and late phase cancer cell apoptosis	666:707	early and late phase cancer cell apoptosis	666:707	8A augmented early and late phase cancer cell apoptosis significantly compared to that of Ebselen and 8B-D; 3.					
29584939	2	1	theme	significant	369:379	arg1	cytotoxicity					381:392	significant cytotoxicity	369:392	significant cytotoxicity in human cancer cells	369:414	However, when Ebselen was conjugated to d-xylose, d-glucose, d-galactose, and d-lactose (8A-D), only Ebselen β-d-xyloside (8A) showed significant cytotoxicity in human cancer cells.					
29584939	8	2	theme	novel	1143:1147	arg1	strategy					1159:1166	novel antitumor strategy	1143:1166	novel antitumor strategy	1143:1166	Thus, 8A represents a lead compound for the development of novel antitumor strategy by targeting glycosaminoglycans.					
29584939	4	3	dep	induced	572:578	arg1	whereas					607:613	whereas	607:613	whereas	607:613	Ebselen induced S phase cell cycle arrest, whereas 8A induced G2/M cell cycle arrest; 2.					
29584939	1	4	from	assembly	209:216	arg1	cells					228:232	animal cells	221:232	animal cells	221:232	Most β-d-xylosides with hydrophobic aglycones are nontoxic primers for glycosaminoglycan assembly in animal cells.					
29584939	5	5	theme	late	676:679	arg1	apoptosis					699:707	early and late phase cancer cell apoptosis	666:707	early and late phase cancer cell apoptosis	666:707	8A augmented early and late phase cancer cell apoptosis significantly compared to that of Ebselen and 8B-D; 3.					
29584939	6	6	theme	disaccharide	835:846	arg1	compositions					848:859	similar disaccharide compositions	827:859	similar disaccharide compositions	827:859	Both 8A and phenyl-β-d-xyloside primed glycosaminoglycans with similar disaccharide compositions in CHO-pgsA745 cells; 4.					
29584939	4	7	theme	G2/M	626:629	arg1	arrest					642:647	G2/M cell cycle arrest; 2	626:650	G2/M cell cycle arrest; 2	626:650	Ebselen induced S phase cell cycle arrest, whereas 8A induced G2/M cell cycle arrest; 2.					
29584939	2	8	theme	Ebselen	336:342	arg1	8A					358:359	8A	358:359	8A	358:359	However, when Ebselen was conjugated to d-xylose, d-glucose, d-galactose, and d-lactose (8A-D), only Ebselen β-d-xyloside (8A) showed significant cytotoxicity in human cancer cells.					
29584939	2	8	theme	Ebselen	336:342	arg1	β-d-xyloside					344:355	only Ebselen β-d-xyloside	331:355	only Ebselen β-d-xyloside (8A)	331:360	However, when Ebselen was conjugated to d-xylose, d-glucose, d-galactose, and d-lactose (8A-D), only Ebselen β-d-xyloside (8A) showed significant cytotoxicity in human cancer cells.					
29584939	5	9	theme	phase	681:685	arg1	apoptosis					699:707	early and late phase cancer cell apoptosis	666:707	early and late phase cancer cell apoptosis	666:707	8A augmented early and late phase cancer cell apoptosis significantly compared to that of Ebselen and 8B-D; 3.					
29584939	6	10	theme	similar	827:833	arg1	compositions					848:859	similar disaccharide compositions	827:859	similar disaccharide compositions	827:859	Both 8A and phenyl-β-d-xyloside primed glycosaminoglycans with similar disaccharide compositions in CHO-pgsA745 cells; 4.					
29584939	1	11	theme	animal	221:226	arg1	cells					228:232	animal cells	221:232	animal cells	221:232	Most β-d-xylosides with hydrophobic aglycones are nontoxic primers for glycosaminoglycan assembly in animal cells.					
29584939	4	12	theme	cell	631:634	arg1	arrest					642:647	G2/M cell cycle arrest; 2	626:650	G2/M cell cycle arrest; 2	626:650	Ebselen induced S phase cell cycle arrest, whereas 8A induced G2/M cell cycle arrest; 2.					
29584939	8	13	theme	lead	1106:1109	arg1	compound					1111:1118	a lead compound	1104:1118	a lead compound for the development of novel antitumor strategy	1104:1166	Thus, 8A represents a lead compound for the development of novel antitumor strategy by targeting glycosaminoglycans.					
29584939	1	14	theme	Most	120:123	arg1	β-d-xylosides					125:137	Most β-d-xylosides	120:137	Most β-d-xylosides with hydrophobic aglycones	120:164	Most β-d-xylosides with hydrophobic aglycones are nontoxic primers for glycosaminoglycan assembly in animal cells.					
29584939	1	14	theme	Most	120:123	arg1	primers					179:185	nontoxic primers	170:185	nontoxic primers for glycosaminoglycan assembly in animal cells	170:232	Most β-d-xylosides with hydrophobic aglycones are nontoxic primers for glycosaminoglycan assembly in animal cells.					
29584939	6	15	dep	primed	796:801	arg1	4					883:883	4	883:883	4	883:883	Both 8A and phenyl-β-d-xyloside primed glycosaminoglycans with similar disaccharide compositions in CHO-pgsA745 cells; 4.					
29584939	2	16	theme	cancer	403:408	arg1	cells					410:414	human cancer cells	397:414	human cancer cells	397:414	However, when Ebselen was conjugated to d-xylose, d-glucose, d-galactose, and d-lactose (8A-D), only Ebselen β-d-xyloside (8A) showed significant cytotoxicity in human cancer cells.					
29584939	0	17	theme	Aglycone	0:7	arg1	Ebselen					9:15	Aglycone Ebselen	0:15	Aglycone Ebselen	0:15	Aglycone Ebselen and β-d-Xyloside Primed Glycosaminoglycans Co-contribute to Ebselen β-d-Xyloside-Induced Cytotoxicity.					
29584939	4	18	theme	cycle	593:597	arg1	arrest					599:604	S phase cell cycle arrest	580:604	S phase cell cycle arrest	580:604	Ebselen induced S phase cell cycle arrest, whereas 8A induced G2/M cell cycle arrest; 2.					
29584939	2	19	theme	only	331:334	arg1	8A					358:359	8A	358:359	8A	358:359	However, when Ebselen was conjugated to d-xylose, d-glucose, d-galactose, and d-lactose (8A-D), only Ebselen β-d-xyloside (8A) showed significant cytotoxicity in human cancer cells.					
29584939	2	19	theme	only	331:334	arg1	β-d-xyloside					344:355	only Ebselen β-d-xyloside	331:355	only Ebselen β-d-xyloside (8A)	331:360	However, when Ebselen was conjugated to d-xylose, d-glucose, d-galactose, and d-lactose (8A-D), only Ebselen β-d-xyloside (8A) showed significant cytotoxicity in human cancer cells.					
29584939	2	20	theme	human	397:401	arg1	cells					410:414	human cancer cells	397:414	human cancer cells	397:414	However, when Ebselen was conjugated to d-xylose, d-glucose, d-galactose, and d-lactose (8A-D), only Ebselen β-d-xyloside (8A) showed significant cytotoxicity in human cancer cells.					
29584939	0	21	theme	β-d-Xyloside	21:32	arg1	Co-contribute					60:72	β-d-Xyloside Primed Glycosaminoglycans Co-contribute	21:72	β-d-Xyloside Primed Glycosaminoglycans Co-contribute	21:72	Aglycone Ebselen and β-d-Xyloside Primed Glycosaminoglycans Co-contribute to Ebselen β-d-Xyloside-Induced Cytotoxicity.					
29584939	4	22	theme	cell	588:591	arg1	arrest					599:604	S phase cell cycle arrest	580:604	S phase cell cycle arrest	580:604	Ebselen induced S phase cell cycle arrest, whereas 8A induced G2/M cell cycle arrest; 2.					
29584939	2	23	from	cytotoxicity	381:392	arg1	cells					410:414	human cancer cells	397:414	human cancer cells	397:414	However, when Ebselen was conjugated to d-xylose, d-glucose, d-galactose, and d-lactose (8A-D), only Ebselen β-d-xyloside (8A) showed significant cytotoxicity in human cancer cells.					
29584939	1	24	with	β-d-xylosides	125:137	arg1	aglycones					156:164	hydrophobic aglycones	144:164	hydrophobic aglycones	144:164	Most β-d-xylosides with hydrophobic aglycones are nontoxic primers for glycosaminoglycan assembly in animal cells.					
29584939	1	25	theme	hydrophobic	144:154	arg1	aglycones					156:164	hydrophobic aglycones	144:164	hydrophobic aglycones	144:164	Most β-d-xylosides with hydrophobic aglycones are nontoxic primers for glycosaminoglycan assembly in animal cells.					
29584939	0	26	theme	Glycosaminoglycans	41:58	arg1	Co-contribute					60:72	β-d-Xyloside Primed Glycosaminoglycans Co-contribute	21:72	β-d-Xyloside Primed Glycosaminoglycans Co-contribute	21:72	Aglycone Ebselen and β-d-Xyloside Primed Glycosaminoglycans Co-contribute to Ebselen β-d-Xyloside-Induced Cytotoxicity.					
29584939	6	27	from	glycosaminoglycans	803:820	arg1	cells					876:880	CHO-pgsA745 cells	864:880	CHO-pgsA745 cells	864:880	Both 8A and phenyl-β-d-xyloside primed glycosaminoglycans with similar disaccharide compositions in CHO-pgsA745 cells; 4.					
29584939	0	28	theme	Primed	34:39	arg1	Co-contribute					60:72	β-d-Xyloside Primed Glycosaminoglycans Co-contribute	21:72	β-d-Xyloside Primed Glycosaminoglycans Co-contribute	21:72	Aglycone Ebselen and β-d-Xyloside Primed Glycosaminoglycans Co-contribute to Ebselen β-d-Xyloside-Induced Cytotoxicity.					
29584939	6	29	with	glycosaminoglycans	803:820	arg1	compositions					848:859	similar disaccharide compositions	827:859	similar disaccharide compositions	827:859	Both 8A and phenyl-β-d-xyloside primed glycosaminoglycans with similar disaccharide compositions in CHO-pgsA745 cells; 4.					
29584939	5	30	theme	cancer	687:692	arg1	apoptosis					699:707	early and late phase cancer cell apoptosis	666:707	early and late phase cancer cell apoptosis	666:707	8A augmented early and late phase cancer cell apoptosis significantly compared to that of Ebselen and 8B-D; 3.					
29584939	3	31	theme	aglycone	456:463	arg1	Ebselen					465:471	the aglycone Ebselen	452:471	the aglycone Ebselen	452:471	The following facts indicated that the aglycone Ebselen and β-d-xyloside primed glycosaminoglycans co-contributed to the observed cytotoxicity: 1.					
29584939	7	32	theme	primed	1057:1062	arg1	glycosaminoglycans					1064:1081	the primed glycosaminoglycans	1053:1081	the primed glycosaminoglycans	1053:1081	Glycosaminoglycans could be detected inside of cells only when treated with 8A, indicating Ebselen contributed to the unique property of intracellular localization of the primed glycosaminoglycans.					
29584939	3	33	theme	following	421:429	arg1	facts					431:435	The following facts	417:435	The following facts	417:435	The following facts indicated that the aglycone Ebselen and β-d-xyloside primed glycosaminoglycans co-contributed to the observed cytotoxicity: 1.					
29584939	4	34	theme	phase	582:586	arg1	arrest					599:604	S phase cell cycle arrest	580:604	S phase cell cycle arrest	580:604	Ebselen induced S phase cell cycle arrest, whereas 8A induced G2/M cell cycle arrest; 2.					
29584939	5	35	theme	cell	694:697	arg1	apoptosis					699:707	early and late phase cancer cell apoptosis	666:707	early and late phase cancer cell apoptosis	666:707	8A augmented early and late phase cancer cell apoptosis significantly compared to that of Ebselen and 8B-D; 3.					
29584939	7	36	theme	localization	1037:1048	arg1	property					1011:1018	the unique property	1000:1018	the unique property of intracellular localization of the primed glycosaminoglycans	1000:1081	Glycosaminoglycans could be detected inside of cells only when treated with 8A, indicating Ebselen contributed to the unique property of intracellular localization of the primed glycosaminoglycans.					
29584939	3	37	theme	observed	538:545	arg1	cytotoxicity					547:558	the observed cytotoxicity: 1	534:561	the observed cytotoxicity: 1	534:561	The following facts indicated that the aglycone Ebselen and β-d-xyloside primed glycosaminoglycans co-contributed to the observed cytotoxicity: 1.					
29584939	4	38	theme	S	580:580	arg1	arrest					599:604	S phase cell cycle arrest	580:604	S phase cell cycle arrest	580:604	Ebselen induced S phase cell cycle arrest, whereas 8A induced G2/M cell cycle arrest; 2.					
29584939	7	39	theme	glycosaminoglycans	1064:1081	arg1	localization					1037:1048	intracellular localization	1023:1048	intracellular localization of the primed glycosaminoglycans	1023:1081	Glycosaminoglycans could be detected inside of cells only when treated with 8A, indicating Ebselen contributed to the unique property of intracellular localization of the primed glycosaminoglycans.					
29584939	1	40	theme	nontoxic	170:177	arg1	β-d-xylosides					125:137	Most β-d-xylosides	120:137	Most β-d-xylosides with hydrophobic aglycones	120:164	Most β-d-xylosides with hydrophobic aglycones are nontoxic primers for glycosaminoglycan assembly in animal cells.					
29584939	1	40	theme	nontoxic	170:177	arg1	primers					179:185	nontoxic primers	170:185	nontoxic primers for glycosaminoglycan assembly in animal cells	170:232	Most β-d-xylosides with hydrophobic aglycones are nontoxic primers for glycosaminoglycan assembly in animal cells.					
29584939	4	41	theme	cycle	636:640	arg1	arrest					642:647	G2/M cell cycle arrest; 2	626:650	G2/M cell cycle arrest; 2	626:650	Ebselen induced S phase cell cycle arrest, whereas 8A induced G2/M cell cycle arrest; 2.					
29584939	8	42	theme	antitumor	1149:1157	arg1	strategy					1159:1166	novel antitumor strategy	1143:1166	novel antitumor strategy	1143:1166	Thus, 8A represents a lead compound for the development of novel antitumor strategy by targeting glycosaminoglycans.					
29584939	6	43	theme	CHO-pgsA745	864:874	arg1	cells					876:880	CHO-pgsA745 cells	864:880	CHO-pgsA745 cells	864:880	Both 8A and phenyl-β-d-xyloside primed glycosaminoglycans with similar disaccharide compositions in CHO-pgsA745 cells; 4.					
29584939	8	44	theme	strategy	1159:1166	arg1	development					1128:1138	the development	1124:1138	the development of novel antitumor strategy	1124:1166	Thus, 8A represents a lead compound for the development of novel antitumor strategy by targeting glycosaminoglycans.					
29584939	3	45	theme	co-contributed	516:529	arg1	glycosaminoglycans					497:514	glycosaminoglycans	497:514	glycosaminoglycans co-contributed to the observed cytotoxicity: 1	497:561	The following facts indicated that the aglycone Ebselen and β-d-xyloside primed glycosaminoglycans co-contributed to the observed cytotoxicity: 1.					
29584939	5	46	dep	that	735:738	arg1	3					761:761	3	761:761	3	761:761	8A augmented early and late phase cancer cell apoptosis significantly compared to that of Ebselen and 8B-D; 3.					
29584939	7	47	theme	intracellular	1023:1035	arg1	localization					1037:1048	intracellular localization	1023:1048	intracellular localization of the primed glycosaminoglycans	1023:1081	Glycosaminoglycans could be detected inside of cells only when treated with 8A, indicating Ebselen contributed to the unique property of intracellular localization of the primed glycosaminoglycans.					
29584939	7	48	theme	unique	1004:1009	arg1	property					1011:1018	the unique property	1000:1018	the unique property of intracellular localization of the primed glycosaminoglycans	1000:1081	Glycosaminoglycans could be detected inside of cells only when treated with 8A, indicating Ebselen contributed to the unique property of intracellular localization of the primed glycosaminoglycans.					
29584939	1	49	theme	glycosaminoglycan	191:207	arg1	assembly					209:216	glycosaminoglycan assembly	191:216	glycosaminoglycan assembly in animal cells	191:232	Most β-d-xylosides with hydrophobic aglycones are nontoxic primers for glycosaminoglycan assembly in animal cells.					
30174308	5	0	theme	LPS-TLR4	803:810	arg1	pathway					812:818	the LPS-TLR4 pathway	799:818	the LPS-TLR4 pathway	799:818	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	6	1	theme	during CR	1164:1172	arg1	therapeutics					1213:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.					
30174308	1	2	theme	fat	176:178	arg1	development					144:154	development	144:154	development of functional beige fat	144:178	Caloric restriction (CR) stimulates development of functional beige fat and extends healthy lifespan.					
30174308	5	3	theme	pathway	812:818	arg1	suppression					784:794	Genetic and pharmacological suppression	756:794	Genetic and pharmacological suppression of the LPS-TLR4 pathway	756:818	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	5	3	theme	pathway	812:818	arg1	transplantation					823:837	transplantation	823:837	transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells	823:890	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	5	4	theme	Tlr4-/-	972:978	arg1	mice					1003:1006	Tlr4-/- or microbiota-depleted mice	972:1006	Tlr4-/- or microbiota-depleted mice	972:1006	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	3	5	theme	lipopolysaccharide	517:534	arg1	component					551:559	a critical lipopolysaccharide (LPS) building component	506:559	a critical lipopolysaccharide (LPS) building component	506:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	3	5	theme	lipopolysaccharide	517:534	arg1	biosynthesis					492:503	the lipid A biosynthesis	480:503	the lipid A biosynthesis	480:503	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	4	6	from	polarization	717:728	arg1	fat					733:735	fat	733:735	fat of the CR animals	733:753	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	6	7	theme	critical	1096:1103	arg1	signals					1088:1094	signals	1088:1094	signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1088:1224	These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.					
30174308	5	8	theme	Genetic	756:762	arg1	suppression					784:794	Genetic and pharmacological suppression	756:794	Genetic and pharmacological suppression of the LPS-TLR4 pathway	756:818	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	6	9	theme	axis	1159:1162	arg1	therapeutics					1213:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.					
30174308	4	10	theme	innate	605:610	arg1	response					619:626	the innate immune response	601:626	the innate immune response	601:626	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	2	11	theme	improvements	334:345	arg1	number					303:308	a number	301:308	a number of CR-induced metabolic improvements	301:345	Here we show that compositional and functional changes in the gut microbiota contribute to a number of CR-induced metabolic improvements and promote fat browning.					
30174308	4	12	from	infiltration	671:682	arg1	fat					733:735	fat	733:735	fat of the CR animals	733:753	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	3	13	theme	critical	508:515	arg1	lipopolysaccharide					517:534	critical lipopolysaccharide	508:534	a critical lipopolysaccharide (LPS) building component	506:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	3	13	theme	critical	508:515	arg1	LPS					537:539	LPS	537:539	LPS	537:539	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	4	14	theme	CR animals	744:753	arg1	fat					733:735	fat	733:735	fat of the CR animals	733:753	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	5	15	theme	pharmacological	768:782	arg1	suppression					784:794	Genetic and pharmacological suppression	756:794	Genetic and pharmacological suppression of the LPS-TLR4 pathway	756:818	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	5	16	theme	fat	908:910	arg1	development					912:922	beige fat development	902:922	beige fat development	902:922	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	5	17	theme	fatty	953:957	arg1	liver					959:963	diet-induced fatty liver	940:963	diet-induced fatty liver	940:963	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	3	18	theme	bacterial	448:456	arg1	enzymes					458:464	the key bacterial enzymes	440:464	the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component	440:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	5	19	theme	Tlr4-/-	844:850	arg1	cells					886:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	5	20	theme	bone-marrow-derived	852:870	arg1	cells					886:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	3	21	theme	building	542:549	arg1	component					551:559	a critical lipopolysaccharide (LPS) building component	506:559	a critical lipopolysaccharide (LPS) building component	506:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	3	21	theme	building	542:549	arg1	biosynthesis					492:503	the lipid A biosynthesis	480:503	the lipid A biosynthesis	480:503	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	2	22	from	changes	257:263	arg1	microbiota					276:285	the gut microbiota	268:285	the gut microbiota	268:285	Here we show that compositional and functional changes in the gut microbiota contribute to a number of CR-induced metabolic improvements and promote fat browning.					
30174308	1	23	theme	healthy	192:198	arg1	lifespan					200:207	healthy lifespan	192:207	healthy lifespan	192:207	Caloric restriction (CR) stimulates development of functional beige fat and extends healthy lifespan.					
30174308	0	24	theme	Gut	11:13	arg1	Remodeling					26:35	Functional Gut Microbiota Remodeling	0:35	Functional Gut Microbiota Remodeling	0:35	Functional Gut Microbiota Remodeling Contributes to the Caloric Restriction-Induced Metabolic Improvements.					
30174308	4	25	theme	eosinophil	660:669	arg1	infiltration					671:682	increased eosinophil infiltration	650:682	increased eosinophil infiltration	650:682	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	1	26	theme	Caloric	108:114	arg1	restriction					116:126	Caloric restriction	108:126	Caloric restriction (CR)	108:131	Caloric restriction (CR) stimulates development of functional beige fat and extends healthy lifespan.					
30174308	1	26	theme	Caloric	108:114	arg1	CR					129:130	CR	129:130	CR	129:130	Caloric restriction (CR) stimulates development of functional beige fat and extends healthy lifespan.					
30174308	4	27	theme	anti-inflammatory	688:704	arg1	polarization					717:728	anti-inflammatory macrophage polarization	688:728	anti-inflammatory macrophage polarization	688:728	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	0	28	theme	Functional	0:9	arg1	Remodeling					26:35	Functional Gut Microbiota Remodeling	0:35	Functional Gut Microbiota Remodeling	0:35	Functional Gut Microbiota Remodeling Contributes to the Caloric Restriction-Induced Metabolic Improvements.					
30174308	4	29	theme	decreased	566:574	arg1	LPS					576:578	The decreased LPS	562:578	The decreased LPS	562:578	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	2	30	theme	metabolic	324:332	arg1	improvements					334:345	CR-induced metabolic improvements	313:345	CR-induced metabolic improvements	313:345	Here we show that compositional and functional changes in the gut microbiota contribute to a number of CR-induced metabolic improvements and promote fat browning.					
30174308	3	31	theme	key	444:446	arg1	enzymes					458:464	the key bacterial enzymes	440:464	the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component	440:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	5	32	theme	hematopoietic	872:884	arg1	cells					886:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	2	33	theme	CR-induced	313:322	arg1	improvements					334:345	CR-induced metabolic improvements	313:345	CR-induced metabolic improvements	313:345	Here we show that compositional and functional changes in the gut microbiota contribute to a number of CR-induced metabolic improvements and promote fat browning.					
30174308	6	34	theme	anti-obesity	1200:1211	arg1	therapeutics					1213:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.					
30174308	5	35	theme	beige	902:906	arg1	development					912:922	beige fat development	902:922	beige fat development	902:922	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	0	36	theme	Microbiota	15:24	arg1	Remodeling					26:35	Functional Gut Microbiota Remodeling	0:35	Functional Gut Microbiota Remodeling	0:35	Functional Gut Microbiota Remodeling Contributes to the Caloric Restriction-Induced Metabolic Improvements.					
30174308	5	37	theme	further	1025:1031	arg1	alterations					1057:1067	further CR-stimulated metabolic alterations	1025:1067	further CR-stimulated metabolic alterations	1025:1067	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	5	38	theme	metabolic	1047:1055	arg1	alterations					1057:1067	further CR-stimulated metabolic alterations	1025:1067	further CR-stimulated metabolic alterations	1025:1067	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	3	39	theme	lipid	484:488	arg1	component					551:559	a critical lipopolysaccharide (LPS) building component	506:559	a critical lipopolysaccharide (LPS) building component	506:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	3	39	theme	lipid	484:488	arg1	biosynthesis					492:503	the lipid A biosynthesis	480:503	the lipid A biosynthesis	480:503	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	4	40	theme	increased	650:658	arg1	infiltration					671:682	increased eosinophil infiltration	650:682	increased eosinophil infiltration	650:682	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	6	41	theme	potential	1186:1194	arg1	therapeutics					1213:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.					
30174308	3	42	theme	necessary	466:474	arg1	enzymes					458:464	the key bacterial enzymes	440:464	the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component	440:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	6	43	theme	new	1196:1198	arg1	therapeutics					1213:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.					
30174308	5	44	theme	diet-induced	940:951	arg1	liver					959:963	diet-induced fatty liver	940:963	diet-induced fatty liver	940:963	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	2	45	theme	functional	246:255	arg1	changes					257:263	compositional and functional changes	228:263	compositional and functional changes in the gut microbiota	228:285	Here we show that compositional and functional changes in the gut microbiota contribute to a number of CR-induced metabolic improvements and promote fat browning.					
30174308	6	46	theme	signaling	1149:1157	arg1	therapeutics					1213:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.					
30174308	0	47	theme	Caloric	56:62	arg1	Improvements					94:105	the Caloric Restriction-Induced Metabolic Improvements	52:105	the Caloric Restriction-Induced Metabolic Improvements	52:105	Functional Gut Microbiota Remodeling Contributes to the Caloric Restriction-Induced Metabolic Improvements.					
30174308	5	48	theme	microbiota-depleted	983:1001	arg1	mice					1003:1006	Tlr4-/- or microbiota-depleted mice	972:1006	Tlr4-/- or microbiota-depleted mice	972:1006	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	3	49	theme	lower	420:424	arg1	expression					426:435	a lower expression	418:435	a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component	418:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	2	50	theme	compositional	228:240	arg1	changes					257:263	compositional and functional changes	228:263	compositional and functional changes in the gut microbiota	228:285	Here we show that compositional and functional changes in the gut microbiota contribute to a number of CR-induced metabolic improvements and promote fat browning.					
30174308	2	51	theme	fat	359:361	arg1	browning					363:370	fat browning	359:370	fat browning	359:370	Here we show that compositional and functional changes in the gut microbiota contribute to a number of CR-induced metabolic improvements and promote fat browning.					
30174308	3	52	attach	linked	408:413	arg2	effects					396:402	these effects	390:402	these effects	390:402	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	3	52	attach	linked	408:413	arg1	expression					426:435	a lower expression	418:435	a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component	418:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	5	53	with	suppression	784:794	arg1	cells					886:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	3	54	theme	A	490:490	arg1	component					551:559	a critical lipopolysaccharide (LPS) building component	506:559	a critical lipopolysaccharide (LPS) building component	506:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	3	54	theme	A	490:490	arg1	biosynthesis					492:503	the lipid A biosynthesis	480:503	the lipid A biosynthesis	480:503	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	5	55	theme	CR-stimulated	1033:1045	arg1	alterations					1057:1067	further CR-stimulated metabolic alterations	1025:1067	further CR-stimulated metabolic alterations	1025:1067	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	6	56	theme	microbiota-fat	1134:1147	arg1	therapeutics					1213:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.					
30174308	5	57	with	transplantation	823:837	arg1	cells					886:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	4	58	dep	response	619:626	arg1	CR					635:636	CR	635:636	CR	635:636	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	2	59	theme	gut	272:274	arg1	microbiota					276:285	the gut microbiota	268:285	the gut microbiota	268:285	Here we show that compositional and functional changes in the gut microbiota contribute to a number of CR-induced metabolic improvements and promote fat browning.					
30174308	4	60	theme	macrophage	706:715	arg1	polarization					717:728	anti-inflammatory macrophage polarization	688:728	anti-inflammatory macrophage polarization	688:728	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	4	61	theme	immune	612:617	arg1	response					619:626	the innate immune response	601:626	the innate immune response	601:626	The decreased LPS dictates the tone of the innate immune response during CR, leading to increased eosinophil infiltration and anti-inflammatory macrophage polarization in fat of the CR animals.					
30174308	0	62	theme	Metabolic	84:92	arg1	Improvements					94:105	the Caloric Restriction-Induced Metabolic Improvements	52:105	the Caloric Restriction-Induced Metabolic Improvements	52:105	Functional Gut Microbiota Remodeling Contributes to the Caloric Restriction-Induced Metabolic Improvements.					
30174308	5	63	link	bone-marrow-derived	852:870	arg1	cells					886:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Tlr4-/- bone-marrow-derived hematopoietic cells	844:890	Genetic and pharmacological suppression of the LPS-TLR4 pathway or transplantation with Tlr4-/- bone-marrow-derived hematopoietic cells increases beige fat development and ameliorates diet-induced fatty liver, while Tlr4-/- or microbiota-depleted mice are resistant to further CR-stimulated metabolic alterations.					
30174308	6	64	theme	and provide	1174:1184	arg1	therapeutics					1213:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics	1134:1224	These data reveal signals critical for our understanding of the microbiota-fat signaling axis during CR and provide potential new anti-obesity therapeutics.					
30174308	1	65	theme	functional	159:168	arg1	fat					176:178	functional beige fat	159:178	functional beige fat	159:178	Caloric restriction (CR) stimulates development of functional beige fat and extends healthy lifespan.					
30174308	3	66	theme	enzymes	458:464	arg1	expression					426:435	a lower expression	418:435	a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component	418:559	Mechanistically, these effects are linked to a lower expression of the key bacterial enzymes necessary for the lipid A biosynthesis, a critical lipopolysaccharide (LPS) building component.					
30174308	0	67	theme	Restriction-Induced	64:82	arg1	Improvements					94:105	the Caloric Restriction-Induced Metabolic Improvements	52:105	the Caloric Restriction-Induced Metabolic Improvements	52:105	Functional Gut Microbiota Remodeling Contributes to the Caloric Restriction-Induced Metabolic Improvements.					
30174308	1	68	theme	beige	170:174	arg1	fat					176:178	functional beige fat	159:178	functional beige fat	159:178	Caloric restriction (CR) stimulates development of functional beige fat and extends healthy lifespan.					
31872872	7	0	theme	carbon	986:991	arg1	JS					973:974	JS	973:974	JS	973:974	JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve).					
31872872	7	0	theme	carbon	986:991	arg1	source					993:998	a rich carbon source	979:998	a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve)	979:1046	JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve).					
31872872	6	1	theme	antioxidant	835:845	arg1	potential					847:855	high antioxidant potential	830:855	high antioxidant potential	830:855	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	1	theme	antioxidant	835:845	arg1	lipids					917:922	lipids	917:922	lipids (12.8% dm)	917:933	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	1	theme	antioxidant	835:845	arg1	traces					965:970	ash traces	961:970	ash traces	961:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	1	theme	antioxidant	835:845	arg1	fibers					875:880	elevated dietary fibers	858:880	elevated dietary fibers (72.7% dm)	858:891	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	1	theme	antioxidant	835:845	arg1	content					948:954	low protein content	936:954	low protein content	936:954	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	1	theme	antioxidant	835:845	arg1	amount					907:912	considerable amount	894:912	considerable amount of lipids (12.8% dm), low protein content, and ash traces	894:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	2	2	theme	fruit	148:152	arg1	pomace					154:159	Juçara fruit pomace	141:159	Juçara fruit pomace	141:159	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	7	3	theme	rich	981:984	arg1	JS					973:974	JS	973:974	JS	973:974	JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve).					
31872872	7	3	theme	rich	981:984	arg1	source					993:998	a rich carbon source	979:998	a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve)	979:1046	JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve).					
31872872	2	4	theme	nutritional	273:283	arg1	content					285:291	their attractive nutritional content	256:291	their attractive nutritional content	256:291	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	8	5	from	high	1055:1058	arg1	carbohydrates					1063:1075	carbohydrates	1063:1075	carbohydrates	1063:1075	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	5	6	theme	phenolic	597:604	arg1	content					606:612	Total phenolic content	591:612	Total phenolic content	591:612	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	6	7	theme	monomeric	738:746	arg1	[dm					814:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	7	theme	monomeric	738:746	arg1	anthocyanins					748:759	monomeric anthocyanins	738:759	monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm])	738:818	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	11	8	theme	nutritive	1417:1425	arg1	source					1427:1432	a nutritive source	1415:1432	a nutritive source of bioactive compounds	1415:1455	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	11	8	theme	nutritive	1417:1425	arg1	pomace					1476:1481	juçara pomace	1469:1481	juçara pomace	1469:1481	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	12	9	theme	fruits	1841:1846	arg1	%					1832:1832	approximately 74%	1816:1832	approximately 74% of the fruits	1816:1846	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	9	theme	fruits	1841:1846	arg1	fruits					1841:1846	the fruits	1837:1846	the fruits	1837:1846	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	7	10	dep	source	993:998	arg1	amylaceous					1028:1037	amylaceous	1028:1037	amylaceous	1028:1037	JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve).					
31872872	6	11	theme	%	887:887	arg1	dm					889:890	72.7% dm	883:890	72.7% dm	883:890	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	11	theme	%	887:887	arg1	fibers					875:880	elevated dietary fibers	858:880	elevated dietary fibers (72.7% dm)	858:891	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	13	12	used	used	1870:1873	arg2	pomace					1856:1861	Juçara pomace	1849:1861	Juçara pomace	1849:1861	Juçara pomace can be used to produce flour with marketing potential due to their functional properties and nutritional value.					
31872872	9	13	theme	thermal	1245:1251	arg1	stability					1253:1261	conventional starch-like thermal stability	1220:1261	conventional starch-like thermal stability	1220:1261	JSS-F presented B-type crystallinity and conventional starch-like thermal stability.					
31872872	15	14	theme	juçara	2192:2197	arg1	seeds					2199:2203	juçara seeds	2192:2203	juçara seeds	2192:2203	Starch can be extracted from juçara seeds, presenting adequate technological properties for partial replacement of conventional starches.					
31872872	2	15	theme	pulp-making	207:217	arg1	process					219:225	the pulp-making process	203:225	the pulp-making process	203:225	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	1	16	theme	starch	109:114	arg1	sources					83:89	sources	83:89	sources of unconventional starch and bioactive compounds	83:138	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	6	17	theme	dietary	867:873	arg1	dm					889:890	72.7% dm	883:890	72.7% dm	883:890	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	17	theme	dietary	867:873	arg1	fibers					875:880	elevated dietary fibers	858:880	elevated dietary fibers (72.7% dm)	858:891	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	17	theme	dietary	867:873	arg1	potential					847:855	high antioxidant potential	830:855	high antioxidant potential	830:855	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	8	18	theme	starch	1122:1127	arg1	[JSS-F					1169:1174	juçara seed starch-flour [JSS-F	1144:1174	juçara seed starch-flour [JSS-F	1144:1174	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	18	theme	starch	1122:1127	arg1	flour					1137:1141	high starch content flour	1117:1141	high starch content flour (juçara seed starch-flour [JSS-F])	1117:1176	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	18	theme	starch	1122:1127	arg1	it					1093:1094	it	1093:1094	it	1093:1094	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	11	19	theme	chemical	1598:1605	arg1	industries					1607:1616	food, pharmaceutical, and chemical industries	1572:1616	food, pharmaceutical, and chemical industries	1572:1616	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	13	20	theme	nutritional	1956:1966	arg1	value					1968:1972	nutritional value	1956:1972	nutritional value	1956:1972	Juçara pomace can be used to produce flour with marketing potential due to their functional properties and nutritional value.					
31872872	1	21	theme	bioactive	120:128	arg1	compounds					130:138	bioactive compounds	120:138	bioactive compounds	120:138	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	6	22	theme	%	929:929	arg1	lipids					917:922	lipids	917:922	lipids (12.8% dm)	917:933	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	22	theme	%	929:929	arg1	dm					931:932	12.8% dm	925:932	12.8% dm	925:932	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	23	from	rich	730:733	arg1	[dm					814:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	23	from	rich	730:733	arg1	anthocyanins					748:759	monomeric anthocyanins	738:759	monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm])	738:818	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	14	24	theme	food	2153:2156	arg1	dye					2158:2160	a natural food dye	2143:2160	a natural food dye	2143:2160	This flour can be incorporated directly into formulations or be used in extraction processes to obtain components of interest, for example, anthocyanins, to be used as a natural food dye.					
31872872	13	25	theme	due	1917:1919	arg1	potential					1907:1915	marketing potential	1897:1915	marketing potential due to their functional properties and nutritional value	1897:1972	Juçara pomace can be used to produce flour with marketing potential due to their functional properties and nutritional value.					
31872872	15	26	theme	starches	2291:2298	arg1	replacement					2263:2273	partial replacement	2255:2273	partial replacement of conventional starches	2255:2298	Starch can be extracted from juçara seeds, presenting adequate technological properties for partial replacement of conventional starches.					
31872872	8	27	theme	juçara	1144:1149	arg1	[JSS-F					1169:1174	juçara seed starch-flour [JSS-F	1144:1174	juçara seed starch-flour [JSS-F	1144:1174	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	27	theme	juçara	1144:1149	arg1	flour					1137:1141	high starch content flour	1117:1141	high starch content flour (juçara seed starch-flour [JSS-F])	1117:1176	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	12	28	dep	APPLICATION	1629:1639	arg1	residues					1664:1671	Juçara agroindustrial residues	1642:1671	Juçara agroindustrial residues (pomace and seeds)	1642:1690	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	28	dep	APPLICATION	1629:1639	arg1	source					1708:1713	a promising source	1696:1713	a promising source	1696:1713	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	15	29	theme	partial	2255:2261	arg1	replacement					2263:2273	partial replacement	2255:2273	partial replacement of conventional starches	2255:2298	Starch can be extracted from juçara seeds, presenting adequate technological properties for partial replacement of conventional starches.					
31872872	8	30	theme	starch-flour	1156:1167	arg1	[JSS-F					1169:1174	juçara seed starch-flour [JSS-F	1144:1174	juçara seed starch-flour [JSS-F	1144:1174	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	30	theme	starch-flour	1156:1167	arg1	flour					1137:1141	high starch content flour	1117:1141	high starch content flour (juçara seed starch-flour [JSS-F])	1117:1176	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	5	31	theme	seedless	700:707	arg1	pomace					709:714	the juçara seedless pomace	689:714	the juçara seedless pomace (JSP)	689:720	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	5	31	theme	seedless	700:707	arg1	JSP					717:719	JSP	717:719	JSP	717:719	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	6	32	theme	considerable	894:905	arg1	potential					847:855	high antioxidant potential	830:855	high antioxidant potential	830:855	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	32	theme	considerable	894:905	arg1	lipids					917:922	lipids	917:922	lipids (12.8% dm)	917:933	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	32	theme	considerable	894:905	arg1	traces					965:970	ash traces	961:970	ash traces	961:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	32	theme	considerable	894:905	arg1	content					948:954	low protein content	936:954	low protein content	936:954	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	32	theme	considerable	894:905	arg1	dm					931:932	12.8% dm	925:932	12.8% dm	925:932	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	32	theme	considerable	894:905	arg1	amount					907:912	considerable amount	894:912	considerable amount of lipids (12.8% dm), low protein content, and ash traces	894:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	5	33	dep	in	641:642	arg1	vitro					644:648	vitro	644:648	vitro	644:648	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	12	34	dep	residues	1664:1671	arg1	residues					1664:1671	Juçara agroindustrial residues	1642:1671	Juçara agroindustrial residues (pomace and seeds)	1642:1690	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	34	dep	residues	1664:1671	arg1	seeds					1685:1689	seeds	1685:1689	seeds	1685:1689	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	34	dep	residues	1664:1671	arg1	pomace					1674:1679	pomace	1674:1679	pomace	1674:1679	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	13	35	theme	marketing	1897:1905	arg1	potential					1907:1915	marketing potential	1897:1915	marketing potential due to their functional properties and nutritional value	1897:1972	Juçara pomace can be used to produce flour with marketing potential due to their functional properties and nutritional value.					
31872872	12	36	theme	starch	1750:1755	arg1	residues					1664:1671	Juçara agroindustrial residues	1642:1671	Juçara agroindustrial residues (pomace and seeds)	1642:1690	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	36	theme	starch	1750:1755	arg1	source					1708:1713	a promising source	1696:1713	a promising source	1696:1713	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	6	37	from	anthocyanins	748:759	arg1	rich					730:733	rich	730:733	rich	730:733	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	3	38	theme	fruit	365:369	arg1	pomace					371:376	juçara fruit pomace	358:376	juçara fruit pomace	358:376	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	10	39	theme	isotherm	1298:1305	arg1	behavior					1307:1314	type III sorption isotherm behavior	1280:1314	type III sorption isotherm behavior	1280:1314	JSS-F exhibited type III sorption isotherm behavior and the Gugghenheim-Anderson-DeBoer model adequately represented the moisture equilibrium data.					
31872872	6	40	theme	content	948:954	arg1	potential					847:855	high antioxidant potential	830:855	high antioxidant potential	830:855	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	40	theme	content	948:954	arg1	lipids					917:922	lipids	917:922	lipids (12.8% dm)	917:933	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	40	theme	content	948:954	arg1	traces					965:970	ash traces	961:970	ash traces	961:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	40	theme	content	948:954	arg1	content					948:954	low protein content	936:954	low protein content	936:954	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	40	theme	content	948:954	arg1	dm					931:932	12.8% dm	925:932	12.8% dm	925:932	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	40	theme	content	948:954	arg1	amount					907:912	considerable amount	894:912	considerable amount of lipids (12.8% dm), low protein content, and ash traces	894:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	41	dep	7.23	770:773	arg1	to					767:768	to	767:768	to	767:768	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	0	42	theme	Euterpe	16:22	arg1	edulis					24:29	Euterpe edulis	16:29	Euterpe edulis	16:29	Valorization of Euterpe edulis Mart.					
31872872	6	43	theme	low	936:938	arg1	content					948:954	low protein content	936:954	low protein content	936:954	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	3	44	theme	alternative	384:394	arg1	potential					345:353	the potential	341:353	the potential of juçara fruit pomace	341:376	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	3	44	theme	alternative	384:394	arg1	source					396:401	an alternative source	381:401	an alternative source of starch and natural dyes	381:428	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	4	45	theme	thermal	531:537	arg1	profile					539:545	thermal profile	531:545	thermal profile	531:545	Starch extracted from juçara seed (JS) was characterized in approximate composition, crystallinity, thermal profile, morphology, and equilibrium moisture data.					
31872872	10	46	theme	Gugghenheim-Anderson-DeBoer	1324:1350	arg1	model					1352:1356	the Gugghenheim-Anderson-DeBoer model	1320:1356	the Gugghenheim-Anderson-DeBoer model	1320:1356	JSS-F exhibited type III sorption isotherm behavior and the Gugghenheim-Anderson-DeBoer model adequately represented the moisture equilibrium data.					
31872872	3	47	theme	dyes	425:428	arg1	potential					345:353	the potential	341:353	the potential of juçara fruit pomace	341:376	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	3	47	theme	dyes	425:428	arg1	source					396:401	an alternative source	381:401	an alternative source of starch and natural dyes	381:428	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	7	48	theme	fibers	1008:1013	arg1	[dm					1015:1017	76.91% fibers [dm]	1001:1018	76.91% fibers [dm]	1001:1018	JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve).					
31872872	6	49	theme	matter	807:812	arg1	[dm					814:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	49	theme	matter	807:812	arg1	anthocyanins					748:759	monomeric anthocyanins	738:759	monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm])	738:818	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	10	50	theme	equilibrium	1394:1404	arg1	data					1406:1409	the moisture equilibrium data	1381:1409	the moisture equilibrium data	1381:1409	JSS-F exhibited type III sorption isotherm behavior and the Gugghenheim-Anderson-DeBoer model adequately represented the moisture equilibrium data.					
31872872	11	51	theme	alternative	1538:1548	arg1	ingredient					1558:1567	an alternative natural ingredient	1535:1567	an alternative natural ingredient to food, pharmaceutical, and chemical industries	1535:1616	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	7	52	theme	76.91	1001:1005	arg1	[dm					1015:1017	76.91% fibers [dm]	1001:1018	76.91% fibers [dm]	1001:1018	JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve).					
31872872	6	53	theme	3-O-glycoside/g	787:801	arg1	[dm					814:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	53	theme	3-O-glycoside/g	787:801	arg1	anthocyanins					748:759	monomeric anthocyanins	738:759	monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm])	738:818	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	0	54	dep	Mart	31:34	arg1	Valorization					0:11	Valorization	0:11	Valorization of Euterpe edulis	0:29	Valorization of Euterpe edulis Mart.					
31872872	2	55	theme	abundant	180:187	arg1	byproducts					189:198	the most abundant byproducts	171:198	the most abundant byproducts of the pulp-making process	171:225	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	9	56	theme	B-type	1195:1200	arg1	crystallinity					1202:1214	B-type crystallinity	1195:1214	B-type crystallinity	1195:1214	JSS-F presented B-type crystallinity and conventional starch-like thermal stability.					
31872872	12	57	theme	promising	1698:1706	arg1	residues					1664:1671	Juçara agroindustrial residues	1642:1671	Juçara agroindustrial residues (pomace and seeds)	1642:1690	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	57	theme	promising	1698:1706	arg1	source					1708:1713	a promising source	1696:1713	a promising source	1696:1713	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	6	58	theme	mg	775:776	arg1	[dm					814:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	58	theme	mg	775:776	arg1	anthocyanins					748:759	monomeric anthocyanins	738:759	monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm])	738:818	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	12	59	theme	agroindustrial	1649:1662	arg1	residues					1664:1671	Juçara agroindustrial residues	1642:1671	Juçara agroindustrial residues (pomace and seeds)	1642:1690	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	59	theme	agroindustrial	1649:1662	arg1	source					1708:1713	a promising source	1696:1713	a promising source	1696:1713	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	59	theme	agroindustrial	1649:1662	arg1	seeds					1685:1689	seeds	1685:1689	seeds	1685:1689	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	59	theme	agroindustrial	1649:1662	arg1	pomace					1674:1679	pomace	1674:1679	pomace	1674:1679	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	4	60	theme	equilibrium	564:574	arg1	data					585:588	equilibrium moisture data	564:588	equilibrium moisture data	564:588	Starch extracted from juçara seed (JS) was characterized in approximate composition, crystallinity, thermal profile, morphology, and equilibrium moisture data.					
31872872	14	61	theme	interest	2092:2099	arg1	components					2078:2087	components	2078:2087	components of interest	2078:2099	This flour can be incorporated directly into formulations or be used in extraction processes to obtain components of interest, for example, anthocyanins, to be used as a natural food dye.					
31872872	11	62	theme	food	1572:1575	arg1	industries					1607:1616	food, pharmaceutical, and chemical industries	1572:1616	food, pharmaceutical, and chemical industries	1572:1616	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	13	63	theme	Juçara	1849:1854	arg1	pomace					1856:1861	Juçara pomace	1849:1861	Juçara pomace	1849:1861	Juçara pomace can be used to produce flour with marketing potential due to their functional properties and nutritional value.					
31872872	6	64	theme	elevated	858:865	arg1	dm					889:890	72.7% dm	883:890	72.7% dm	883:890	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	64	theme	elevated	858:865	arg1	fibers					875:880	elevated dietary fibers	858:880	elevated dietary fibers (72.7% dm)	858:891	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	64	theme	elevated	858:865	arg1	potential					847:855	high antioxidant potential	830:855	high antioxidant potential	830:855	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	5	65	theme	anthocyanins	615:626	arg1	content					628:634	anthocyanins content	615:634	anthocyanins content	615:634	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	9	66	theme	starch-like	1233:1243	arg1	stability					1253:1261	conventional starch-like thermal stability	1220:1261	conventional starch-like thermal stability	1220:1261	JSS-F presented B-type crystallinity and conventional starch-like thermal stability.					
31872872	12	67	theme	PRACTICAL	1619:1627	arg1	APPLICATION					1629:1639	PRACTICAL APPLICATION	1619:1639	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.	1619:1847	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	6	68	theme	traces	965:970	arg1	potential					847:855	high antioxidant potential	830:855	high antioxidant potential	830:855	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	68	theme	traces	965:970	arg1	lipids					917:922	lipids	917:922	lipids (12.8% dm)	917:933	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	68	theme	traces	965:970	arg1	traces					965:970	ash traces	961:970	ash traces	961:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	68	theme	traces	965:970	arg1	content					948:954	low protein content	936:954	low protein content	936:954	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	68	theme	traces	965:970	arg1	dm					931:932	12.8% dm	925:932	12.8% dm	925:932	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	68	theme	traces	965:970	arg1	amount					907:912	considerable amount	894:912	considerable amount of lipids (12.8% dm), low protein content, and ash traces	894:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	69	theme	high	830:833	arg1	potential					847:855	high antioxidant potential	830:855	high antioxidant potential	830:855	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	69	theme	high	830:833	arg1	lipids					917:922	lipids	917:922	lipids (12.8% dm)	917:933	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	69	theme	high	830:833	arg1	traces					965:970	ash traces	961:970	ash traces	961:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	69	theme	high	830:833	arg1	fibers					875:880	elevated dietary fibers	858:880	elevated dietary fibers (72.7% dm)	858:891	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	69	theme	high	830:833	arg1	content					948:954	low protein content	936:954	low protein content	936:954	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	69	theme	high	830:833	arg1	amount					907:912	considerable amount	894:912	considerable amount of lipids (12.8% dm), low protein content, and ash traces	894:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	70	dep	rich	730:733	arg1	presents					821:828	presents	821:828	presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces	821:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	14	71	theme	extraction	2047:2056	arg1	processes					2058:2066	extraction processes	2047:2066	extraction processes	2047:2066	This flour can be incorporated directly into formulations or be used in extraction processes to obtain components of interest, for example, anthocyanins, to be used as a natural food dye.					
31872872	2	72	theme	Juçara	141:146	arg1	pomace					154:159	Juçara fruit pomace	141:159	Juçara fruit pomace	141:159	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	5	73	theme	Total	591:595	arg1	content					606:612	Total phenolic content	591:612	Total phenolic content	591:612	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	2	74	theme	attractive	262:271	arg1	content					285:291	their attractive nutritional content	256:291	their attractive nutritional content	256:291	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	11	75	theme	bioactive	1437:1445	arg1	compounds					1447:1455	bioactive compounds	1437:1455	bioactive compounds	1437:1455	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	7	76	dep	amylaceous	1028:1037	arg1	[dm					1015:1017	76.91% fibers [dm]	1001:1018	76.91% fibers [dm]	1001:1018	JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve).					
31872872	2	77	dep	discarded	238:246	arg1	one					164:166	one	164:166	one	164:166	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	2	77	dep	discarded	238:246	arg1	byproducts					189:198	the most abundant byproducts	171:198	the most abundant byproducts of the pulp-making process	171:225	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	1	78	dep	residues	52:59	arg1	seeds					73:77	seeds	73:77	seeds	73:77	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	1	78	dep	residues	52:59	arg1	sources					83:89	sources	83:89	sources of unconventional starch and bioactive compounds	83:138	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	1	78	dep	residues	52:59	arg1	pomace					62:67	pomace	62:67	pomace	62:67	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	1	78	dep	residues	52:59	arg1	residues					52:59	agroindustrial residues	37:59	agroindustrial residues (pomace and seeds)	37:78	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	2	79	theme	process	219:225	arg1	byproducts					189:198	the most abundant byproducts	171:198	the most abundant byproducts of the pulp-making process	171:225	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	5	80	theme	in	641:642	arg1	capacity					662:669	in vitro antioxidant capacity	641:669	in vitro antioxidant capacity	641:669	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	1	81	theme	unconventional	94:107	arg1	starch					109:114	unconventional starch	94:114	unconventional starch	94:114	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	11	82	theme	pharmaceutical	1578:1591	arg1	industries					1607:1616	food, pharmaceutical, and chemical industries	1572:1616	food, pharmaceutical, and chemical industries	1572:1616	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	5	83	theme	antioxidant	650:660	arg1	capacity					662:669	in vitro antioxidant capacity	641:669	in vitro antioxidant capacity	641:669	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	8	84	from	carbohydrates	1063:1075	arg1	high					1055:1058	high	1055:1058	high	1055:1058	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	85	theme	high	1117:1120	arg1	[JSS-F					1169:1174	juçara seed starch-flour [JSS-F	1144:1174	juçara seed starch-flour [JSS-F	1144:1174	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	85	theme	high	1117:1120	arg1	flour					1137:1141	high starch content flour	1117:1141	high starch content flour (juçara seed starch-flour [JSS-F])	1117:1176	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	85	theme	high	1117:1120	arg1	it					1093:1094	it	1093:1094	it	1093:1094	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	1	86	theme	compounds	130:138	arg1	sources					83:89	sources	83:89	sources of unconventional starch and bioactive compounds	83:138	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	8	87	theme	content	1129:1135	arg1	[JSS-F					1169:1174	juçara seed starch-flour [JSS-F	1144:1174	juçara seed starch-flour [JSS-F	1144:1174	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	87	theme	content	1129:1135	arg1	flour					1137:1141	high starch content flour	1117:1141	high starch content flour (juçara seed starch-flour [JSS-F])	1117:1176	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	87	theme	content	1129:1135	arg1	it					1093:1094	it	1093:1094	it	1093:1094	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	14	88	theme	natural	2145:2151	arg1	dye					2158:2160	a natural food dye	2143:2160	a natural food dye	2143:2160	This flour can be incorporated directly into formulations or be used in extraction processes to obtain components of interest, for example, anthocyanins, to be used as a natural food dye.					
31872872	15	89	theme	conventional	2278:2289	arg1	starches					2291:2298	conventional starches	2278:2298	conventional starches	2278:2298	Starch can be extracted from juçara seeds, presenting adequate technological properties for partial replacement of conventional starches.					
31872872	6	90	theme	lipids	917:922	arg1	potential					847:855	high antioxidant potential	830:855	high antioxidant potential	830:855	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	90	theme	lipids	917:922	arg1	lipids					917:922	lipids	917:922	lipids (12.8% dm)	917:933	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	90	theme	lipids	917:922	arg1	traces					965:970	ash traces	961:970	ash traces	961:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	90	theme	lipids	917:922	arg1	content					948:954	low protein content	936:954	low protein content	936:954	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	90	theme	lipids	917:922	arg1	dm					931:932	12.8% dm	925:932	12.8% dm	925:932	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	90	theme	lipids	917:922	arg1	amount					907:912	considerable amount	894:912	considerable amount of lipids (12.8% dm), low protein content, and ash traces	894:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	10	91	theme	type	1280:1283	arg1	behavior					1307:1314	type III sorption isotherm behavior	1280:1314	type III sorption isotherm behavior	1280:1314	JSS-F exhibited type III sorption isotherm behavior and the Gugghenheim-Anderson-DeBoer model adequately represented the moisture equilibrium data.					
31872872	5	92	theme	juçara	693:698	arg1	pomace					709:714	the juçara seedless pomace	689:714	the juçara seedless pomace (JSP)	689:720	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	5	92	theme	juçara	693:698	arg1	JSP					717:719	JSP	717:719	JSP	717:719	Total phenolic content, anthocyanins content, and in vitro antioxidant capacity were assessed for the juçara seedless pomace (JSP).					
31872872	13	93	theme	functional	1930:1939	arg1	properties					1941:1950	their functional properties	1924:1950	their functional properties	1924:1950	Juçara pomace can be used to produce flour with marketing potential due to their functional properties and nutritional value.					
31872872	8	94	theme	seed	1151:1154	arg1	[JSS-F					1169:1174	juçara seed starch-flour [JSS-F	1144:1174	juçara seed starch-flour [JSS-F	1144:1174	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	8	94	theme	seed	1151:1154	arg1	flour					1137:1141	high starch content flour	1117:1141	high starch content flour (juçara seed starch-flour [JSS-F])	1117:1176	Being high in carbohydrates, mainly starch, it can be classified as high starch content flour (juçara seed starch-flour [JSS-F]).					
31872872	6	95	theme	ash	961:963	arg1	traces					965:970	ash traces	961:970	ash traces	961:970	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	3	96	theme	natural	417:423	arg1	dyes					425:428	natural dyes	417:428	natural dyes	417:428	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	15	97	theme	technological	2226:2238	arg1	properties					2240:2249	adequate technological properties	2217:2249	adequate technological properties	2217:2249	Starch can be extracted from juçara seeds, presenting adequate technological properties for partial replacement of conventional starches.					
31872872	3	98	theme	pomace	371:376	arg1	source					396:401	an alternative source	381:401	an alternative source of starch and natural dyes	381:428	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	3	98	theme	pomace	371:376	arg1	potential					345:353	the potential	341:353	the potential of juçara fruit pomace	341:376	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	10	99	theme	sorption	1289:1296	arg1	behavior					1307:1314	type III sorption isotherm behavior	1280:1314	type III sorption isotherm behavior	1280:1314	JSS-F exhibited type III sorption isotherm behavior and the Gugghenheim-Anderson-DeBoer model adequately represented the moisture equilibrium data.					
31872872	3	100	theme	starch	406:411	arg1	potential					345:353	the potential	341:353	the potential of juçara fruit pomace	341:376	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	3	100	theme	starch	406:411	arg1	source					396:401	an alternative source	381:401	an alternative source of starch and natural dyes	381:428	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	0	101	theme	edulis	24:29	arg1	Valorization					0:11	Valorization	0:11	Valorization of Euterpe edulis	0:29	Valorization of Euterpe edulis Mart.					
31872872	12	102	theme	antioxidants	1718:1729	arg1	residues					1664:1671	Juçara agroindustrial residues	1642:1671	Juçara agroindustrial residues (pomace and seeds)	1642:1690	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	102	theme	antioxidants	1718:1729	arg1	source					1708:1713	a promising source	1696:1713	a promising source	1696:1713	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	6	103	theme	protein	940:946	arg1	content					948:954	low protein content	936:954	low protein content	936:954	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	3	104	theme	juçara	358:363	arg1	pomace					371:376	juçara fruit pomace	358:376	juçara fruit pomace	358:376	In this sense, this study aimed to investigate the potential of juçara fruit pomace as an alternative source of starch and natural dyes.					
31872872	11	105	theme	natural	1550:1556	arg1	ingredient					1558:1567	an alternative natural ingredient	1535:1567	an alternative natural ingredient to food, pharmaceutical, and chemical industries	1535:1616	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	7	106	theme	%	1006:1006	arg1	[dm					1015:1017	76.91% fibers [dm]	1001:1018	76.91% fibers [dm]	1001:1018	JS is a rich carbon source (76.91% fibers [dm]; 12.21% amylaceous reserve).					
31872872	12	107	theme	unconventional	1735:1748	arg1	starch					1750:1755	unconventional starch	1735:1755	unconventional starch	1735:1755	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	4	108	theme	approximate	491:501	arg1	composition					503:513	approximate composition	491:513	approximate composition	491:513	Starch extracted from juçara seed (JS) was characterized in approximate composition, crystallinity, thermal profile, morphology, and equilibrium moisture data.					
31872872	6	109	theme	dry	803:805	arg1	[dm					814:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	109	theme	dry	803:805	arg1	anthocyanins					748:759	monomeric anthocyanins	738:759	monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm])	738:818	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	15	110	theme	adequate	2217:2224	arg1	properties					2240:2249	adequate technological properties	2217:2249	adequate technological properties	2217:2249	Starch can be extracted from juçara seeds, presenting adequate technological properties for partial replacement of conventional starches.					
31872872	10	111	theme	moisture	1385:1392	arg1	data					1406:1409	the moisture equilibrium data	1381:1409	the moisture equilibrium data	1381:1409	JSS-F exhibited type III sorption isotherm behavior and the Gugghenheim-Anderson-DeBoer model adequately represented the moisture equilibrium data.					
31872872	2	112	theme	byproducts	189:198	arg1	one					164:166	one	164:166	one	164:166	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	2	112	theme	byproducts	189:198	arg1	byproducts					189:198	the most abundant byproducts	171:198	the most abundant byproducts of the pulp-making process	171:225	Juçara fruit pomace is one of the most abundant byproducts of the pulp-making process, generally discarded despite their attractive nutritional content.					
31872872	11	113	theme	compounds	1447:1455	arg1	starch					1461:1466	starch	1461:1466	starch	1461:1466	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	11	113	theme	compounds	1447:1455	arg1	source					1427:1432	a nutritive source	1415:1432	a nutritive source of bioactive compounds	1415:1455	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	11	113	theme	compounds	1447:1455	arg1	pomace					1476:1481	juçara pomace	1469:1481	juçara pomace	1469:1481	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	14	114	used	used	2039:2042	arg2	flour					1980:1984	This flour	1975:1984	This flour	1975:1984	This flour can be incorporated directly into formulations or be used in extraction processes to obtain components of interest, for example, anthocyanins, to be used as a natural food dye.					
31872872	6	115	theme	cyanidin	778:785	arg1	[dm					814:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm	762:816	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	6	115	theme	cyanidin	778:785	arg1	anthocyanins					748:759	monomeric anthocyanins	738:759	monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm])	738:818	JSP is rich in monomeric anthocyanins (7.19 to 7.23 mg cyanidin 3-O-glycoside/g dry matter [dm]), presents high antioxidant potential, elevated dietary fibers (72.7% dm), considerable amount of lipids (12.8% dm), low protein content, and ash traces.					
31872872	4	116	theme	moisture	576:583	arg1	data					585:588	equilibrium moisture data	564:588	equilibrium moisture data	564:588	Starch extracted from juçara seed (JS) was characterized in approximate composition, crystallinity, thermal profile, morphology, and equilibrium moisture data.					
31872872	1	117	theme	agroindustrial	37:50	arg1	seeds					73:77	seeds	73:77	seeds	73:77	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	1	117	theme	agroindustrial	37:50	arg1	pomace					62:67	pomace	62:67	pomace	62:67	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	1	117	theme	agroindustrial	37:50	arg1	residues					52:59	agroindustrial residues	37:59	agroindustrial residues (pomace and seeds)	37:78	agroindustrial residues (pomace and seeds) as sources of unconventional starch and bioactive compounds.					
31872872	12	118	theme	Juçara	1642:1647	arg1	residues					1664:1671	Juçara agroindustrial residues	1642:1671	Juçara agroindustrial residues (pomace and seeds)	1642:1690	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	118	theme	Juçara	1642:1647	arg1	source					1708:1713	a promising source	1696:1713	a promising source	1696:1713	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	118	theme	Juçara	1642:1647	arg1	seeds					1685:1689	seeds	1685:1689	seeds	1685:1689	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	12	118	theme	Juçara	1642:1647	arg1	pomace					1674:1679	pomace	1674:1679	pomace	1674:1679	PRACTICAL APPLICATION: Juçara agroindustrial residues (pomace and seeds) are a promising source of antioxidants and unconventional starch, which are usually discarded after depulping, representing approximately 74% of the fruits.					
31872872	11	119	theme	juçara	1469:1474	arg1	starch					1461:1466	starch	1461:1466	starch	1461:1466	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	11	119	theme	juçara	1469:1474	arg1	pomace					1476:1481	juçara pomace	1469:1481	juçara pomace	1469:1481	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	11	119	theme	juçara	1469:1474	arg1	coproduct					1507:1515	a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries	1505:1616	a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries	1505:1616	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	11	119	theme	juçara	1469:1474	arg1	source					1427:1432	a nutritive source	1415:1432	a nutritive source of bioactive compounds	1415:1455	As a nutritive source of bioactive compounds and starch, juçara pomace should be regarded as a coproduct to be explored as an alternative natural ingredient to food, pharmaceutical, and chemical industries.					
31872872	9	120	theme	conventional	1220:1231	arg1	stability					1253:1261	conventional starch-like thermal stability	1220:1261	conventional starch-like thermal stability	1220:1261	JSS-F presented B-type crystallinity and conventional starch-like thermal stability.					
31207953	1	0	attach	isolated	356:363	arg2	glycosides					208:217	Seven sulfated triterpene glycosides	182:217	Seven sulfated triterpene glycosides	182:217	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	1	0	attach	isolated	356:363	arg1	cucumber					378:385	the sea cucumber	370:385	the sea cucumber Psolus fabricii collected in the Sea of Okhotsk	370:433	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	2	1	theme	data	603:606	arg1	application					557:567	application	557:567	application of modern NMR and particularly MS data	557:606	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	0	2	theme	Corrected	106:114	arg1	Structure					116:124	the Corrected Structure	102:124	the Corrected Structure of Psolusoside B	102:141	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	1	3	theme	sulfated	188:195	arg1	glycosides					208:217	Seven sulfated triterpene glycosides	182:217	Seven sulfated triterpene glycosides	182:217	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	6	4	theme	tetrasaccharide	1175:1189	arg1	moiety					1215:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety	1149:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit	1149:1241	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	0	5	dep	Cucumber	156:163	arg1	fabricii					172:179	the Sea Cucumber Psolus fabricii	148:179	the Sea Cucumber Psolus fabricii	148:179	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	3	6	theme	NMR	707:709	arg1	spectroscopy					711:722	NMR spectroscopy	707:722	NMR spectroscopy	707:722	The structures of other glycosides were elucidated by 2D NMR spectroscopy and HR-ESI mass-spectrometry.					
31207953	0	7	theme	B	141:141	arg1	G					82:82	G	82:82	G	82:82	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	7	theme	B	141:141	arg1	I					96:96	I	96:96	I	96:96	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	7	theme	B	141:141	arg1	H					85:85	H	85:85	H	85:85	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	7	theme	B	141:141	arg1	H1					88:89	H1	88:89	H1	88:89	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	7	theme	B	141:141	arg1	Structure					116:124	the Corrected Structure	102:124	the Corrected Structure of Psolusoside B	102:141	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	7	theme	B	141:141	arg1	F					79:79	F	79:79	F	79:79	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	7	theme	B	141:141	arg1	Glycosides					51:60	Six New Triterpene Glycosides	32:60	Six New Triterpene Glycosides	32:60	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	5	8	theme	unusual	1033:1039	arg1	moiety					1077:1082	an unusual sulfated trisaccharide carbohydrate moiety	1030:1082	an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit	1030:1124	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	6	9	theme	unprecedented	1152:1164	arg1	moiety					1215:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety	1149:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit	1149:1241	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	5	10	theme	sugar	1115:1119	arg1	unit					1121:1124	the second sugar unit	1104:1124	the second sugar unit	1104:1124	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	5	11	theme	trisaccharide	1050:1062	arg1	moiety					1077:1082	an unusual sulfated trisaccharide carbohydrate moiety	1030:1082	an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit	1030:1124	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	7	12	theme	Ehrlich	1627:1633	arg1	carcinoma					1635:1643	Ehrlich carcinoma	1627:1643	Ehrlich carcinoma	1627:1643	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	7	13	theme	Neuro	1407:1411	arg1	2A					1413:1414	neuroblastoma Neuro 2A	1393:1414	neuroblastoma Neuro 2A	1393:1414	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	9	14	theme	human	1809:1813	arg1	cells					1847:1851	human colorectal adenocarcinoma HT-29 cells	1809:1851	human colorectal adenocarcinoma HT-29 cells	1809:1851	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	1	15	theme	Okhotsk	427:433	arg1	Sea					420:422	the Sea	416:422	the Sea of Okhotsk	416:433	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	9	16	theme	adenocarcinoma	1826:1839	arg1	cells					1847:1851	human colorectal adenocarcinoma HT-29 cells	1809:1851	human colorectal adenocarcinoma HT-29 cells	1809:1851	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	10	17	theme	highest	1978:1984	arg1	activities					1997:2006	The highest inhibitory activities	1974:2006	The highest inhibitory activities	1974:2006	The highest inhibitory activities were demonstrated by psolusosides E (2) and F (3).					
31207953	6	18	theme	xylose	1231:1236	arg1	unit					1238:1241	the xylose unit	1227:1241	the xylose unit	1227:1241	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	4	19	theme	sugar	863:867	arg1	compositions					869:880	their sugar compositions	857:880	their sugar compositions	857:880	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	1	20	dep	glycosides	208:217	arg1	psolusosides					220:231	psolusosides	220:231	psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D	220:344	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	9	21	from	influence	1861:1869	arg1	formation					1885:1893	colony formation	1878:1893	colony formation	1878:1893	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	9	21	from	influence	1861:1869	arg1	growth					1899:1904	growth	1899:1904	growth	1899:1904	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	9	22	dep	compounds	1924:1932	arg1	A					1959:1959	psolusoside A	1947:1959	psolusoside A	1947:1959	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	9	22	dep	compounds	1924:1932	arg1	1-3					1934:1936	1-3	1934:1936	1-3	1934:1936	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	9	22	dep	compounds	1924:1932	arg1	5-7					1939:1941	5-7	1939:1941	5-7	1939:1941	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	4	23	theme	holostane	799:807	arg1	aglycones					809:817	holostane aglycones	799:817	holostane aglycones identical to each other	799:841	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	6	24	theme	carbohydrate	1202:1213	arg1	moiety					1215:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety	1149:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit	1149:1241	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	2	25	theme	psolusoside	461:471	arg1	B					473:473	psolusoside B	461:473	psolusoside B (1)	461:477	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	2	25	theme	psolusoside	461:471	arg1	1					476:476	1	476:476	1	476:476	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	9	26	theme	colony	1878:1883	arg1	formation					1885:1893	colony formation	1878:1893	colony formation	1878:1893	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	0	27	theme	E	76:76	arg1	Bioactivities					15:27	Bioactivities	15:27	Bioactivities	15:27	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	27	theme	E	76:76	arg1	Structures					0:9	Structures	0:9	Structures	0:9	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	28	theme	Psolus	165:170	arg1	Cucumber					156:163	the Sea Cucumber Psolus fabricii	148:179	the Sea Cucumber Psolus fabricii	148:179	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	7	29	theme	mouse	1342:1346	arg1	lines-ascite					1353:1364	several mouse cell lines-ascite	1334:1364	several mouse cell lines-ascite	1334:1364	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	0	30	theme	F	79:79	arg1	Bioactivities					15:27	Bioactivities	15:27	Bioactivities	15:27	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	30	theme	F	79:79	arg1	Structures					0:9	Structures	0:9	Structures	0:9	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	6	31	theme	chain	1273:1277	arg1	position					1257:1264	the second position	1246:1264	the second position of the chain	1246:1277	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	4	32	from	quantity	890:897	arg1	moieties					969:976	linear tetrasaccharide carbohydrate moieties	933:976	linear tetrasaccharide carbohydrate moieties	933:976	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	10	33	theme	psolusosides	2029:2040	arg1	2					2045:2045	2	2045:2045	2	2045:2045	The highest inhibitory activities were demonstrated by psolusosides E (2) and F (3).					
31207953	10	33	theme	psolusosides	2029:2040	arg1	E					2042:2042	psolusosides E	2029:2042	psolusosides E (2)	2029:2046	The highest inhibitory activities were demonstrated by psolusosides E (2) and F (3).					
31207953	1	34	theme	known	306:310	arg1	A					324:324	earlier known psolusoside A	298:324	earlier known psolusoside A	298:324	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	6	35	with	moiety	1215:1220	arg1	unit					1238:1241	the xylose unit	1227:1241	the xylose unit	1227:1241	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	7	36	theme	compounds	1312:1320	arg1	activities					1294:1303	The cytotoxic activities	1280:1303	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite	1280:1364	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	5	37	theme	second	1108:1113	arg1	unit					1121:1124	the second sugar unit	1104:1124	the second sugar unit	1104:1124	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	9	38	theme	cells	1915:1919	arg1	formation					1885:1893	colony formation	1878:1893	colony formation	1878:1893	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	9	38	theme	cells	1915:1919	arg1	growth					1899:1904	growth	1899:1904	growth	1899:1904	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	6	39	contain	has	1145:1147	arg2	moiety					1215:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety	1149:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit	1149:1241	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	6	39	contain	has	1145:1147	arg1	7					1142:1142	7	1142:1142	7	1142:1142	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	6	39	contain	has	1145:1147	arg1	I					1139:1139	Psolusoside I	1127:1139	Psolusoside I (7)	1127:1143	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	6	40	theme	second	1250:1255	arg1	position					1257:1264	the second position	1246:1264	the second position of the chain	1246:1277	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	1	41	dep	along	287:291	arg1	with					293:296	with	293:296	with	293:296	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	8	42	theme	normal	1705:1710	arg1	cells					1717:1721	normal JB-6 cells	1705:1721	normal JB-6 cells	1705:1721	Interestingly, psolusoside G (4) was not cytotoxic against normal JB-6 cells but demonstrated high activity against Neuro 2A cells.					
31207953	7	43	theme	epithelial	1424:1433	arg1	cells					1440:1444	normal epithelial JB-6 cells	1417:1444	normal epithelial JB-6 cells	1417:1444	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	1	44	theme	colochiroside	330:342	arg1	D					344:344	colochiroside D	330:344	colochiroside D	330:344	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	9	45	theme	compounds	1924:1932	arg1	formation					1885:1893	colony formation	1878:1893	colony formation	1878:1893	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	9	45	theme	compounds	1924:1932	arg1	growth					1899:1904	growth	1899:1904	growth	1899:1904	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	7	46	theme	cytotoxic	1284:1292	arg1	activities					1294:1303	The cytotoxic activities	1280:1303	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite	1280:1364	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	7	47	theme	compounds	1526:1534	arg1	effects					1504:1510	hemolytic effects	1494:1510	hemolytic effects of the tested compounds	1494:1534	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	4	48	from	groups	923:928	arg1	moieties					969:976	linear tetrasaccharide carbohydrate moieties	933:976	linear tetrasaccharide carbohydrate moieties	933:976	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	49	theme	tetrasaccharide	940:954	arg1	moieties					969:976	linear tetrasaccharide carbohydrate moieties	933:976	linear tetrasaccharide carbohydrate moieties	933:976	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	0	50	theme	Triterpene	40:49	arg1	Glycosides					51:60	Six New Triterpene Glycosides	32:60	Six New Triterpene Glycosides	32:60	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	5	51	with	moiety	1077:1082	arg1	glucose					1093:1099	the glucose	1089:1099	the glucose as the second sugar unit	1089:1124	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	4	52	contain	contain	791:797	arg1	F					774:774	F (3)	774:778	F (3)	774:778	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	52	contain	contain	791:797	arg1	E					767:767	E (2)	767:771	E (2)	767:771	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	52	contain	contain	791:797	arg1	G					785:785	G (4)	785:789	G (4)	785:789	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	52	contain	contain	791:797	arg2	aglycones					809:817	holostane aglycones	799:817	holostane aglycones identical to each other	799:841	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	52	contain	contain	791:797	arg1	Psolusosides					754:765	Psolusosides E (2), F (3), and G (4)	754:789	Psolusosides E (2), F (3), and G (4)	754:789	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	7	53	theme	different	1475:1483	arg1	carcinoma					1382:1390	Ehrlich carcinoma	1374:1390	Ehrlich carcinoma	1374:1390	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	6	54	theme	Psolusoside	1127:1137	arg1	7					1142:1142	7	1142:1142	7	1142:1142	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	6	54	theme	Psolusoside	1127:1137	arg1	I					1139:1139	Psolusoside I	1127:1139	Psolusoside I (7)	1127:1143	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	5	55	dep	Psolusosides	979:990	arg1	Psolusosides					979:990	Psolusosides H (5) and H1 (6)	979:1007	Psolusosides H (5) and H1 (6)	979:1007	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	5	55	dep	Psolusosides	979:990	arg1	H1					1002:1003	H1 (6)	1002:1007	H1 (6)	1002:1007	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	5	55	dep	Psolusosides	979:990	arg1	H					992:992	H (5)	992:996	H (5)	992:996	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	8	56	theme	Neuro	1762:1766	arg1	cells					1771:1775	Neuro 2A cells	1762:1775	Neuro 2A cells	1762:1775	Interestingly, psolusoside G (4) was not cytotoxic against normal JB-6 cells but demonstrated high activity against Neuro 2A cells.					
31207953	2	57	theme	monosulfated	510:521	arg1	tetraoside					523:532	a monosulfated tetraoside	508:532	a monosulfated tetraoside	508:532	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	2	58	theme	modern	572:577	arg1	NMR					579:581	modern NMR	572:581	modern NMR	572:581	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	4	59	theme	sulfate	915:921	arg1	groups					923:928	sulfate groups	915:928	sulfate groups in linear tetrasaccharide carbohydrate moieties	915:976	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	3	60	theme	glycosides	674:683	arg1	structures					654:663	The structures	650:663	The structures of other glycosides	650:683	The structures of other glycosides were elucidated by 2D NMR spectroscopy and HR-ESI mass-spectrometry.					
31207953	0	61	theme	Structure	116:124	arg1	Bioactivities					15:27	Bioactivities	15:27	Bioactivities	15:27	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	61	theme	Structure	116:124	arg1	Structures					0:9	Structures	0:9	Structures	0:9	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	4	62	dep	quantity	890:897	arg1	the					886:888	the	886:888	the	886:888	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	9	63	from	activity	1792:1799	arg1	formation					1885:1893	colony formation	1878:1893	colony formation	1878:1893	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	9	63	from	activity	1792:1799	arg1	growth					1899:1904	growth	1899:1904	growth	1899:1904	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	7	64	theme	JB-6	1435:1438	arg1	cells					1440:1444	normal epithelial JB-6 cells	1417:1444	normal epithelial JB-6 cells	1417:1444	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	2	65	theme	MS	600:601	arg1	data					603:606	modern NMR and particularly MS data	572:606	modern NMR and particularly MS data	572:606	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	0	66	theme	Psolusoside	129:139	arg1	B					141:141	Psolusoside B	129:141	Psolusoside B	129:141	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	4	67	theme	identical	819:827	arg1	aglycones					809:817	holostane aglycones	799:817	holostane aglycones identical to each other	799:841	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	1	68	theme	triterpene	197:206	arg1	glycosides					208:217	Seven sulfated triterpene glycosides	182:217	Seven sulfated triterpene glycosides	182:217	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	7	69	theme	normal	1417:1422	arg1	cells					1440:1444	normal epithelial JB-6 cells	1417:1444	normal epithelial JB-6 cells	1417:1444	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	4	70	dep	Psolusosides	754:765	arg1	F					774:774	F (3)	774:778	F (3)	774:778	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	70	dep	Psolusosides	754:765	arg1	E					767:767	E (2)	767:771	E (2)	767:771	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	70	dep	Psolusosides	754:765	arg1	G					785:785	G (4)	785:789	G (4)	785:789	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	70	dep	Psolusosides	754:765	arg1	Psolusosides					754:765	Psolusosides E (2), F (3), and G (4)	754:789	Psolusosides E (2), F (3), and G (4)	754:789	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	6	71	theme	branched	1166:1173	arg1	moiety					1215:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety	1149:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit	1149:1241	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	9	72	theme	cytotoxic	1782:1790	arg1	activity					1792:1799	The cytotoxic activity	1778:1799	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells	1778:1851	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	0	73	theme	Sea	152:154	arg1	Cucumber					156:163	the Sea Cucumber Psolus fabricii	148:179	the Sea Cucumber Psolus fabricii	148:179	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	5	74	theme	sulfated	1041:1048	arg1	moiety					1077:1082	an unusual sulfated trisaccharide carbohydrate moiety	1030:1082	an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit	1030:1124	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	3	75	theme	HR-ESI	728:733	arg1	mass-spectrometry					735:751	HR-ESI mass-spectrometry	728:751	HR-ESI mass-spectrometry	728:751	The structures of other glycosides were elucidated by 2D NMR spectroscopy and HR-ESI mass-spectrometry.					
31207953	7	76	theme	neuroblastoma	1393:1405	arg1	2A					1413:1414	neuroblastoma Neuro 2A	1393:1414	neuroblastoma Neuro 2A	1393:1414	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	5	77	theme	carbohydrate	1064:1075	arg1	moiety					1077:1082	an unusual sulfated trisaccharide carbohydrate moiety	1030:1082	an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit	1030:1124	Psolusosides H (5) and H1 (6) are characterized by an unusual sulfated trisaccharide carbohydrate moiety with the glucose as the second sugar unit.					
31207953	7	78	theme	carcinoma	1635:1643	arg1	ascites					1616:1622	the ascites	1612:1622	the ascites of Ehrlich carcinoma	1612:1643	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	9	79	theme	colorectal	1815:1824	arg1	cells					1847:1851	human colorectal adenocarcinoma HT-29 cells	1809:1851	human colorectal adenocarcinoma HT-29 cells	1809:1851	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	7	80	dep	compounds	1312:1320	arg1	2-7					1322:1324	2-7	1322:1324	2-7	1322:1324	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	9	81	theme	HT-29	1841:1845	arg1	cells					1847:1851	human colorectal adenocarcinoma HT-29 cells	1809:1851	human colorectal adenocarcinoma HT-29 cells	1809:1851	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	0	82	from	Cucumber	156:163	arg1	Bioactivities					15:27	Bioactivities	15:27	Bioactivities	15:27	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	82	from	Cucumber	156:163	arg1	Structures					0:9	Structures	0:9	Structures	0:9	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	7	83	theme	Ehrlich	1374:1380	arg1	carcinoma					1382:1390	Ehrlich carcinoma	1374:1390	Ehrlich carcinoma	1374:1390	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	2	84	theme	B	473:473	arg1	structure					448:456	the structure	444:456	the structure	444:456	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	2	84	theme	B	473:473	arg1	tetraoside					638:647	a disulfated tetraoside	625:647	a disulfated tetraoside	625:647	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	1	85	theme	sea	374:376	arg1	cucumber					378:385	the sea cucumber	370:385	the sea cucumber Psolus fabricii collected in the Sea of Okhotsk	370:433	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	6	86	theme	disulfated	1191:1200	arg1	moiety					1215:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety	1149:1220	an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit	1149:1241	Psolusoside I (7) has an unprecedented branched tetrasaccharide disulfated carbohydrate moiety with the xylose unit in the second position of the chain.					
31207953	10	87	theme	inhibitory	1986:1995	arg1	activities					1997:2006	The highest inhibitory activities	1974:2006	The highest inhibitory activities	1974:2006	The highest inhibitory activities were demonstrated by psolusosides E (2) and F (3).					
31207953	7	88	theme	other	1580:1584	arg1	cells					1586:1590	other cells	1580:1590	other cells	1580:1590	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	7	89	theme	cell	1348:1351	arg1	lines-ascite					1353:1364	several mouse cell lines-ascite	1334:1364	several mouse cell lines-ascite	1334:1364	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	1	90	theme	earlier	298:304	arg1	A					324:324	earlier known psolusoside A	298:324	earlier known psolusoside A	298:324	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	7	91	theme	several	1334:1340	arg1	lines-ascite					1353:1364	several mouse cell lines-ascite	1334:1364	several mouse cell lines-ascite	1334:1364	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	1	92	theme	psolusoside	312:322	arg1	A					324:324	earlier known psolusoside A	298:324	earlier known psolusoside A	298:324	Seven sulfated triterpene glycosides, psolusosides B (1), E (2), F (3), G (4), H (5), H1 (6), and I (7), along with earlier known psolusoside A and colochiroside D have been isolated from the sea cucumber Psolus fabricii collected in the Sea of Okhotsk.					
31207953	9	93	theme	HT-29	1909:1913	arg1	cells					1915:1919	HT-29 cells	1909:1919	HT-29 cells	1909:1919	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	8	94	theme	psolusoside	1661:1671	arg1	G					1673:1673	psolusoside G	1661:1673	psolusoside G (4)	1661:1677	Interestingly, psolusoside G (4) was not cytotoxic against normal JB-6 cells but demonstrated high activity against Neuro 2A cells.					
31207953	8	94	theme	psolusoside	1661:1671	arg1	4					1676:1676	4	1676:1676	4	1676:1676	Interestingly, psolusoside G (4) was not cytotoxic against normal JB-6 cells but demonstrated high activity against Neuro 2A cells.					
31207953	4	95	from	compositions	869:880	arg1	moieties					969:976	linear tetrasaccharide carbohydrate moieties	933:976	linear tetrasaccharide carbohydrate moieties	933:976	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	8	96	theme	JB-6	1712:1715	arg1	cells					1717:1721	normal JB-6 cells	1705:1721	normal JB-6 cells	1705:1721	Interestingly, psolusoside G (4) was not cytotoxic against normal JB-6 cells but demonstrated high activity against Neuro 2A cells.					
31207953	4	97	from	moieties	969:976	arg1	position					903:910	position	903:910	position	903:910	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	97	from	moieties	969:976	arg1	compositions					869:880	their sugar compositions	857:880	their sugar compositions	857:880	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	97	from	moieties	969:976	arg1	quantity					890:897	quantity	890:897	quantity	890:897	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	98	theme	linear	933:938	arg1	moieties					969:976	linear tetrasaccharide carbohydrate moieties	933:976	linear tetrasaccharide carbohydrate moieties	933:976	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	0	99	theme	New	36:38	arg1	Glycosides					51:60	Six New Triterpene Glycosides	32:60	Six New Triterpene Glycosides	32:60	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	7	100	theme	tested	1519:1524	arg1	compounds					1526:1534	the tested compounds	1515:1534	the tested compounds	1515:1534	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	8	101	theme	high	1740:1743	arg1	activity					1745:1752	high activity	1740:1752	high activity against Neuro 2A cells	1740:1775	Interestingly, psolusoside G (4) was not cytotoxic against normal JB-6 cells but demonstrated high activity against Neuro 2A cells.					
31207953	4	102	theme	carbohydrate	956:967	arg1	moieties					969:976	linear tetrasaccharide carbohydrate moieties	933:976	linear tetrasaccharide carbohydrate moieties	933:976	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	0	103	theme	Glycosides	51:60	arg1	Bioactivities					15:27	Bioactivities	15:27	Bioactivities	15:27	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	0	103	theme	Glycosides	51:60	arg1	Structures					0:9	Structures	0:9	Structures	0:9	Structures and Bioactivities of Six New Triterpene Glycosides, Psolusosides E, F, G, H, H1, and I and the Corrected Structure of Psolusoside B from the Sea Cucumber Psolus fabricii.					
31207953	3	104	theme	other	668:672	arg1	glycosides					674:683	other glycosides	668:683	other glycosides	668:683	The structures of other glycosides were elucidated by 2D NMR spectroscopy and HR-ESI mass-spectrometry.					
31207953	2	105	theme	disulfated	627:636	arg1	structure					448:456	the structure	444:456	the structure	444:456	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	2	105	theme	disulfated	627:636	arg1	tetraoside					638:647	a disulfated tetraoside	625:647	a disulfated tetraoside	625:647	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	9	106	theme	psolusoside	1947:1957	arg1	A					1959:1959	psolusoside A	1947:1959	psolusoside A	1947:1959	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31207953	7	107	theme	hemolytic	1494:1502	arg1	effects					1504:1510	hemolytic effects	1494:1510	hemolytic effects of the tested compounds	1494:1534	The cytotoxic activities of the compounds 2-7 against several mouse cell lines-ascite form of Ehrlich carcinoma, neuroblastoma Neuro 2A, normal epithelial JB-6 cells, and erythrocytes-were quite different, at that hemolytic effects of the tested compounds were higher than their cytotoxicity against other cells, especially against the ascites of Ehrlich carcinoma.					
31207953	8	108	theme	2A	1768:1769	arg1	cells					1771:1775	Neuro 2A cells	1762:1775	Neuro 2A cells	1762:1775	Interestingly, psolusoside G (4) was not cytotoxic against normal JB-6 cells but demonstrated high activity against Neuro 2A cells.					
31207953	2	109	theme	NMR	579:581	arg1	data					603:606	modern NMR and particularly MS data	572:606	modern NMR and particularly MS data	572:606	Herein, the structure of psolusoside B (1), elucidated by us in 1989 as a monosulfated tetraoside, has been revised with application of modern NMR and particularly MS data and proved to be a disulfated tetraoside.					
31207953	4	110	from	position	903:910	arg1	moieties					969:976	linear tetrasaccharide carbohydrate moieties	933:976	linear tetrasaccharide carbohydrate moieties	933:976	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	111	theme	groups	923:928	arg1	position					903:910	position	903:910	position	903:910	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	111	theme	groups	923:928	arg1	compositions					869:880	their sugar compositions	857:880	their sugar compositions	857:880	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	4	111	theme	groups	923:928	arg1	quantity					890:897	quantity	890:897	quantity	890:897	Psolusosides E (2), F (3), and G (4) contain holostane aglycones identical to each other and differ in their sugar compositions and the quantity and position of sulfate groups in linear tetrasaccharide carbohydrate moieties.					
31207953	9	112	dep	formation	1885:1893	arg1	the					1874:1876	the	1874:1876	the	1874:1876	The cytotoxic activity against human colorectal adenocarcinoma HT-29 cells and the influence on the colony formation and growth of HT-29 cells of compounds 1-3, 5-7 and psolusoside A was checked.					
31325356	9	0	theme	N	1375:1375	arg1	excretion					1352:1360	Fecal excretion	1346:1360	Fecal excretion of microbial N	1346:1375	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	12	1	theme	fecal	2038:2042	arg1	excretion					2044:2052	fecal excretion	2038:2052	fecal excretion of butyrate	2038:2064	Amylase administration increased fecal excretion of butyrate (P = 0.04) and tended to increase isovalerate excretion (P = 0.06).					
31325356	0	2	theme	fatty	149:153	arg1	acids					155:159	volatile fatty acids	140:159	volatile fatty acids in heifers1	140:171	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	1	3	theme	possible	374:381	arg1	limitation					383:392	a possible limitation	372:392	a possible limitation of starch digestion	372:412	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	11	4	theme	increasing	1767:1776	arg1	excretion					1785:1793	the increasing starch excretion	1763:1793	the increasing starch excretion with feces	1763:1804	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	7	5	theme	Titanium	1178:1185	arg1	dioxide					1187:1193	Titanium dioxide	1178:1193	Titanium dioxide	1178:1193	Titanium dioxide was ruminally administered (10 g/d) to estimate fecal excretion.					
31325356	13	6	dep	kg/d	2394:2397	arg1	up					2386:2387	up	2386:2387	up	2386:2387	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	13	7	theme	microbial	2185:2193	arg1	N					2195:2195	microbial N	2185:2195	microbial N	2185:2195	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	2	8	theme	565	545:547	arg1	kg					553:554	565 ± 6 kg	545:554	565 ± 6 kg	545:554	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	11	9	theme	starch	1930:1935	arg1	digestion					1937:1945	starch digestion	1930:1945	starch digestion	1930:1945	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	1	10	theme	digestion	404:412	arg1	limitation					383:392	a possible limitation	372:392	a possible limitation of starch digestion	372:412	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	10	11	from	tract	1614:1618	arg1	digestibility					1563:1575	the apparent digestibility	1550:1575	the apparent digestibility of starch from the total postruminal tract	1550:1618	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	10	12	dep	2	1721:1721	arg1	to					1718:1719	to	1718:1719	to	1718:1719	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	2	13	theme	negligible	614:623	arg1	starch					635:640	starch	635:640	starch	635:640	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	2	13	theme	negligible	614:623	arg1	amount					625:630	only a negligible amount	607:630	only a negligible amount of starch	607:640	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	9	14	theme	infusion	1428:1435	arg1	level					1412:1416	level	1412:1416	level of starch infusion (P < 0.001)	1412:1447	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	11	15	theme	small	1954:1958	arg1	intestine					1960:1968	the small intestine	1950:1968	the small intestine	1950:1968	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	1	16	from	effect	220:225	arg1	disappearance					263:275	postruminal disappearance	251:275	postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers	251:355	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	13	17	theme	starch	2308:2313	arg1	digestion					2315:2323	postruminal starch digestion	2296:2323	postruminal starch digestion in heifers	2296:2334	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	9	18	theme	P	1438:1438	arg1	infusion					1428:1435	starch infusion	1421:1435	starch infusion (P < 0.001)	1421:1447	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	9	18	theme	P	1438:1438	arg1	<					1440:1440	P < 0.001	1438:1446	P < 0.001	1438:1446	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	13	19	theme	starch	2200:2205	arg1	excretion					2172:2180	fecal excretion	2166:2180	fecal excretion of microbial N or starch	2166:2205	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	1	20	theme	heifers	349:355	arg1	abomasum					337:344	the abomasum	333:344	the abomasum of heifers	333:355	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	13	21	theme	pancreatic	2224:2233	arg1	activity					2243:2250	pancreatic amylase activity	2224:2250	pancreatic amylase activity	2224:2250	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	13	21	theme	pancreatic	2224:2233	arg1	factor					2286:2291	the primarily limiting factor	2263:2291	the primarily limiting factor of postruminal starch digestion in heifers	2263:2334	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	3	22	with	trial	689:693	arg1	periods					715:721	2 experimental periods	700:721	2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection	700:804	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	12	23	theme	Amylase	2005:2011	arg1	administration					2013:2026	Amylase administration	2005:2026	Amylase administration	2005:2026	Amylase administration increased fecal excretion of butyrate (P = 0.04) and tended to increase isovalerate excretion (P = 0.06).					
31325356	2	24	theme	diet	582:585	arg1	DM/d					572:575	5.6 kg DM/d	565:575	5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch	565:640	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	3	25	theme	35	731:732	arg1	d					734:734	d	734:734	d	734:734	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	2	26	theme	initial	531:537	arg1	BW					539:540	an initial BW	528:540	an initial BW of 565 ± 6 kg	528:554	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	4	27	theme	sampling	818:825	arg1	period					827:832	the sampling period	814:832	the sampling period	814:832	During the sampling period, each animal was abomasally infused with native corn starch at 5 levels (953, 1,213, 1,425, 1,733, and 1,993 g DM/d) each for a 5-d period with and without exogenous amylase, respectively.					
31325356	10	28	theme	intestinal	1676:1685	arg1	supply					1694:1699	the intestinal starch supply	1672:1699	the intestinal starch supply	1672:1699	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	11	29	theme	starch	1861:1866	arg1	proportion					1839:1848	a constant proportion	1828:1848	a constant proportion of infused starch	1828:1866	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	0	30	theme	starch	69:74	arg1	starch					69:74	corn starch	64:74	corn starch	64:74	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	0	30	theme	starch	69:74	arg1	amylase					30:36	abomasally infused amylase	11:36	abomasally infused amylase	11:36	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	0	30	theme	starch	69:74	arg1	amounts					53:59	increasing amounts	42:59	increasing amounts of corn starch	42:74	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	1	31	theme	corn	302:305	arg1	starch					307:312	corn starch	302:312	corn starch	302:312	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	4	32	dep	levels	899:904	arg1	each					951:954	each	951:954	each	951:954	During the sampling period, each animal was abomasally infused with native corn starch at 5 levels (953, 1,213, 1,425, 1,733, and 1,993 g DM/d) each for a 5-d period with and without exogenous amylase, respectively.					
31325356	0	33	from	Effects	0:6	arg1	excretion					85:93	fecal excretion	79:93	fecal excretion of starch	79:103	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	0	33	from	Effects	0:6	arg1	acids					155:159	volatile fatty acids	140:159	volatile fatty acids in heifers1	140:171	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	0	33	from	Effects	0:6	arg1	nitrogen					126:133	total and microbial nitrogen	106:133	total and microbial nitrogen	106:133	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	2	34	theme	kg	553:554	arg1	BW					539:540	an initial BW	528:540	an initial BW of 565 ± 6 kg	528:554	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	0	35	theme	fecal	79:83	arg1	excretion					85:93	fecal excretion	79:93	fecal excretion of starch	79:103	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	3	36	theme	adaption	759:766	arg1	d					749:749	10 d	746:749	10 d of diet adaption followed by 25 d of sample collection	746:804	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	9	37	theme	starch	1498:1503	arg1	proportion					1472:1481	a constant proportion	1461:1481	a constant proportion of the infused starch being fermented in the hindgut	1461:1534	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	8	38	theme	Purine	1260:1265	arg1	bases					1267:1271	Purine bases	1260:1271	Purine bases in feces	1260:1280	Purine bases in feces were determined and used as a marker for microbial N excretion.					
31325356	8	38	theme	Purine	1260:1265	arg1	marker					1312:1317	a marker	1310:1317	a marker for microbial N excretion	1310:1343	Purine bases in feces were determined and used as a marker for microbial N excretion.					
31325356	2	39	theme	±	549:549	arg1	kg					553:554	565 ± 6 kg	545:554	565 ± 6 kg	545:554	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	13	40	theme	N	2195:2195	arg1	excretion					2172:2180	fecal excretion	2166:2180	fecal excretion of microbial N or starch	2166:2205	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	10	41	theme	starch	1687:1692	arg1	supply					1694:1699	the intestinal starch supply	1672:1699	the intestinal starch supply	1672:1699	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	2	42	theme	kg	569:570	arg1	DM/d					572:575	5.6 kg DM/d	565:575	5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch	565:640	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	6	43	dep	times	1135:1139	arg1	day					1143:1145	a day	1141:1145	4 times a day	1133:1145	Feces were sampled 4 times a day during the collection periods.					
31325356	11	44	from	hindgut	1891:1897	arg1	intestine					1960:1968	the small intestine	1950:1968	the small intestine	1950:1968	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	4	45	theme	corn	882:885	arg1	starch					887:892	native corn starch	875:892	native corn starch	875:892	During the sampling period, each animal was abomasally infused with native corn starch at 5 levels (953, 1,213, 1,425, 1,733, and 1,993 g DM/d) each for a 5-d period with and without exogenous amylase, respectively.					
31325356	4	46	theme	exogenous	990:998	arg1	amylase					1000:1006	exogenous amylase	990:1006	exogenous amylase	990:1006	During the sampling period, each animal was abomasally infused with native corn starch at 5 levels (953, 1,213, 1,425, 1,733, and 1,993 g DM/d) each for a 5-d period with and without exogenous amylase, respectively.					
31325356	13	47	theme	amylase	2235:2241	arg1	activity					2243:2250	pancreatic amylase activity	2224:2250	pancreatic amylase activity	2224:2250	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	13	47	theme	amylase	2235:2241	arg1	factor					2286:2291	the primarily limiting factor	2263:2291	the primarily limiting factor of postruminal starch digestion in heifers	2263:2334	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	1	48	theme	amylase	240:246	arg1	effect					220:225	the effect	216:225	the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers	216:355	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	2	49	dep	heifers	460:466	arg1	heifers					460:466	Four rumen-fistulated heifers	438:466	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg	438:554	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	2	49	dep	heifers	460:466	arg1	Jersey					495:500	Jersey	495:500	Jersey	495:500	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	2	49	dep	heifers	460:466	arg1	German					471:476	German	471:476	German	471:476	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	12	50	theme	isovalerate	2100:2110	arg1	excretion					2112:2120	isovalerate excretion	2100:2120	isovalerate excretion	2100:2120	Amylase administration increased fecal excretion of butyrate (P = 0.04) and tended to increase isovalerate excretion (P = 0.06).					
31325356	8	51	used	used	1302:1305	arg2	bases					1267:1271	Purine bases	1260:1271	Purine bases in feces	1260:1280	Purine bases in feces were determined and used as a marker for microbial N excretion.					
31325356	8	51	used	used	1302:1305	arg2	marker					1312:1317	a marker	1310:1317	a marker for microbial N excretion	1310:1343	Purine bases in feces were determined and used as a marker for microbial N excretion.					
31325356	5	52	theme	starch	1084:1089	arg1	infusion					1072:1079	an abomasal infusion	1060:1079	an abomasal infusion of starch in amounts of 724 g/d	1060:1111	At days 6 to 10 the heifers received an abomasal infusion of starch in amounts of 724 g/d.					
31325356	0	53	theme	amylase	30:36	arg1	Effects					0:6	Effects	0:6	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.	0:172	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	1	54	theme	postruminal	251:261	arg1	disappearance					263:275	postruminal disappearance	251:275	postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers	251:355	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	11	55	theme	constant	1830:1837	arg1	proportion					1839:1848	a constant proportion	1828:1848	a constant proportion of infused starch	1828:1866	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	0	56	theme	increasing	42:51	arg1	starch					69:74	corn starch	64:74	corn starch	64:74	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	0	56	theme	increasing	42:51	arg1	amounts					53:59	increasing amounts	42:59	increasing amounts of corn starch	42:74	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	7	57	dep	administered	1209:1220	arg1	g/d					1226:1228	10 g/d	1223:1228	10 g/d	1223:1228	Titanium dioxide was ruminally administered (10 g/d) to estimate fecal excretion.					
31325356	3	58	theme	sample	788:793	arg1	collection					795:804	sample collection	788:804	sample collection	788:804	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	8	59	theme	microbial	1323:1331	arg1	excretion					1335:1343	microbial N excretion	1323:1343	microbial N excretion	1323:1343	Purine bases in feces were determined and used as a marker for microbial N excretion.					
31325356	10	60	theme	starch	1580:1585	arg1	digestibility					1563:1575	the apparent digestibility	1550:1575	the apparent digestibility of starch from the total postruminal tract	1550:1618	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	1	61	theme	study	197:201	arg1	aim					178:180	The aim	174:180	The aim of the present study	174:201	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	8	62	from	bases	1267:1271	arg1	feces					1276:1280	feces	1276:1280	feces	1276:1280	Purine bases in feces were determined and used as a marker for microbial N excretion.					
31325356	9	63	theme	microbial	1365:1373	arg1	N					1375:1375	microbial N	1365:1375	microbial N	1365:1375	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	13	64	theme	fecal	2166:2170	arg1	excretion					2172:2180	fecal excretion	2166:2180	fecal excretion of microbial N or starch	2166:2205	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	11	65	theme	starch	1778:1783	arg1	excretion					1785:1793	the increasing starch excretion	1763:1793	the increasing starch excretion with feces	1763:1804	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	10	66	theme	postruminal	1602:1612	arg1	tract					1614:1618	the total postruminal tract	1592:1618	the total postruminal tract	1592:1618	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	2	67	theme	starch	635:640	arg1	starch					635:640	starch	635:640	starch	635:640	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	2	67	theme	starch	635:640	arg1	amount					625:630	only a negligible amount	607:630	only a negligible amount of starch	607:640	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	0	68	from	nitrogen	126:133	arg1	heifers1					164:171	heifers1	164:171	heifers1	164:171	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	10	69	theme	%	1646:1646	arg1	P					1656:1656	P < 0.001	1656:1664	P < 0.001	1656:1664	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	10	69	theme	%	1646:1646	arg1	%					1653:1653	90% to 80%	1644:1653	90% to 80% (P < 0.001)	1644:1665	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	1	70	theme	starch	397:402	arg1	digestion					404:412	starch digestion	397:412	starch digestion	397:412	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	4	71	theme	g	943:943	arg1	DM/d					945:948	953, 1,213, 1,425, 1,733, and 1,993 g DM/d	907:948	953, 1,213, 1,425, 1,733, and 1,993 g DM/d	907:948	During the sampling period, each animal was abomasally infused with native corn starch at 5 levels (953, 1,213, 1,425, 1,733, and 1,993 g DM/d) each for a 5-d period with and without exogenous amylase, respectively.					
31325356	4	71	theme	g	943:943	arg1	levels					899:904	5 levels	897:904	5 levels (953, 1,213, 1,425, 1,733, and 1,993 g DM/d) each	897:954	During the sampling period, each animal was abomasally infused with native corn starch at 5 levels (953, 1,213, 1,425, 1,733, and 1,993 g DM/d) each for a 5-d period with and without exogenous amylase, respectively.					
31325356	13	72	theme	corn	2341:2344	arg1	starch					2346:2351	corn starch	2341:2351	corn starch	2341:2351	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	9	73	theme	starch	1421:1426	arg1	infusion					1428:1435	starch infusion	1421:1435	starch infusion (P < 0.001)	1421:1447	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	9	73	theme	starch	1421:1426	arg1	<					1440:1440	P < 0.001	1438:1446	P < 0.001	1438:1446	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	5	74	dep	days	1026:1029	arg1	to					1033:1034	to	1033:1034	to	1033:1034	At days 6 to 10 the heifers received an abomasal infusion of starch in amounts of 724 g/d.					
31325356	11	75	with	indication	1814:1823	arg1	feces					1800:1804	feces	1800:1804	feces	1800:1804	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	0	76	theme	total	106:110	arg1	nitrogen					126:133	total and microbial nitrogen	106:133	total and microbial nitrogen	106:133	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	1	77	theme	small	421:425	arg1	intestine					427:435	the small intestine	417:435	the small intestine	417:435	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	13	78	theme	postruminal	2296:2306	arg1	digestion					2315:2323	postruminal starch digestion	2296:2323	postruminal starch digestion in heifers	2296:2334	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	11	79	theme	digestion	1937:1945	arg1	efficiency					1916:1925	a decreasing efficiency	1903:1925	a decreasing efficiency of starch digestion	1903:1945	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	0	80	theme	microbial	116:124	arg1	nitrogen					126:133	total and microbial nitrogen	106:133	total and microbial nitrogen	106:133	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	13	81	theme	digestion	2315:2323	arg1	activity					2243:2250	pancreatic amylase activity	2224:2250	pancreatic amylase activity	2224:2250	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	13	81	theme	digestion	2315:2323	arg1	factor					2286:2291	the primarily limiting factor	2263:2291	the primarily limiting factor of postruminal starch digestion in heifers	2263:2334	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	13	82	from	digestion	2315:2323	arg1	heifers					2328:2334	heifers	2328:2334	heifers	2328:2334	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	4	83	theme	5-d	962:964	arg1	period					966:971	a 5-d period	960:971	a 5-d period	960:971	During the sampling period, each animal was abomasally infused with native corn starch at 5 levels (953, 1,213, 1,425, 1,733, and 1,993 g DM/d) each for a 5-d period with and without exogenous amylase, respectively.					
31325356	5	84	from	infusion	1072:1079	arg1	amounts					1094:1100	amounts	1094:1100	amounts of 724 g/d	1094:1111	At days 6 to 10 the heifers received an abomasal infusion of starch in amounts of 724 g/d.					
31325356	5	84	from	infusion	1072:1079	arg1	g/d					1109:1111	724 g/d	1105:1111	724 g/d	1105:1111	At days 6 to 10 the heifers received an abomasal infusion of starch in amounts of 724 g/d.					
31325356	10	85	theme	<	1658:1658	arg1	P					1656:1656	P < 0.001	1656:1664	P < 0.001	1656:1664	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	10	85	theme	<	1658:1658	arg1	%					1653:1653	90% to 80%	1644:1653	90% to 80% (P < 0.001)	1644:1665	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	0	86	theme	volatile	140:147	arg1	acids					155:159	volatile fatty acids	140:159	volatile fatty acids in heifers1	140:171	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	11	87	theme	strong	1738:1743	arg1	evidence					1745:1752	strong evidence	1738:1752	strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply	1738:2002	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	0	88	from	excretion	85:93	arg1	heifers1					164:171	heifers1	164:171	heifers1	164:171	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	3	89	theme	crossover	679:687	arg1	trial					689:693	a crossover trial	677:693	a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection	677:804	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	0	90	theme	corn	64:67	arg1	starch					69:74	corn starch	64:74	corn starch	64:74	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	9	91	theme	constant	1463:1470	arg1	proportion					1472:1481	a constant proportion	1461:1481	a constant proportion of the infused starch being fermented in the hindgut	1461:1534	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	13	92	from	heifers	2328:2334	arg1	activity					2243:2250	pancreatic amylase activity	2224:2250	pancreatic amylase activity	2224:2250	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	13	92	from	heifers	2328:2334	arg1	factor					2286:2291	the primarily limiting factor	2263:2291	the primarily limiting factor of postruminal starch digestion in heifers	2263:2334	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	13	93	theme	limiting	2277:2284	arg1	activity					2243:2250	pancreatic amylase activity	2224:2250	pancreatic amylase activity	2224:2250	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	13	93	theme	limiting	2277:2284	arg1	factor					2286:2291	the primarily limiting factor	2263:2291	the primarily limiting factor of postruminal starch digestion in heifers	2263:2334	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	3	94	theme	experimental	702:713	arg1	periods					715:721	2 experimental periods	700:721	2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection	700:804	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	1	95	theme	starch	307:312	arg1	amounts					291:297	increasing amounts	280:297	increasing amounts of corn starch	280:312	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	1	95	theme	starch	307:312	arg1	starch					307:312	corn starch	302:312	corn starch	302:312	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	9	96	theme	infused	1490:1496	arg1	starch					1498:1503	the infused starch	1486:1503	the infused starch being fermented in the hindgut	1486:1534	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	10	97	dep	%	1653:1653	arg1	to					1648:1649	to	1648:1649	to	1648:1649	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	0	98	theme	starch	98:103	arg1	excretion					85:93	fecal excretion	79:93	fecal excretion of starch	79:103	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	0	98	theme	starch	98:103	arg1	acids					155:159	volatile fatty acids	140:159	volatile fatty acids in heifers1	140:171	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	0	98	theme	starch	98:103	arg1	nitrogen					126:133	total and microbial nitrogen	106:133	total and microbial nitrogen	106:133	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	3	99	dep	d	734:734	arg1	each					736:739	each	736:739	each	736:739	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	12	100	theme	butyrate	2057:2064	arg1	excretion					2044:2052	fecal excretion	2038:2052	fecal excretion of butyrate	2038:2064	Amylase administration increased fecal excretion of butyrate (P = 0.04) and tended to increase isovalerate excretion (P = 0.06).					
31325356	3	101	with	d	734:734	arg1	d					749:749	10 d	746:749	10 d of diet adaption followed by 25 d of sample collection	746:804	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	2	102	with	heifers	460:466	arg1	BW					539:540	an initial BW	528:540	an initial BW of 565 ± 6 kg	528:554	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	3	103	theme	diet	754:757	arg1	adaption					759:766	diet adaption	754:766	diet adaption followed by 25 d of sample collection	754:804	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	5	104	theme	abomasal	1063:1070	arg1	infusion					1072:1079	an abomasal infusion	1060:1079	an abomasal infusion of starch in amounts of 724 g/d	1060:1111	At days 6 to 10 the heifers received an abomasal infusion of starch in amounts of 724 g/d.					
31325356	0	105	theme	infused	22:28	arg1	amylase					30:36	abomasally infused amylase	11:36	abomasally infused amylase	11:36	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	1	106	theme	exogenous	230:238	arg1	amylase					240:246	exogenous amylase	230:246	exogenous amylase	230:246	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	4	107	theme	native	875:880	arg1	starch					887:892	native corn starch	875:892	native corn starch	875:892	During the sampling period, each animal was abomasally infused with native corn starch at 5 levels (953, 1,213, 1,425, 1,733, and 1,993 g DM/d) each for a 5-d period with and without exogenous amylase, respectively.					
31325356	0	108	from	acids	155:159	arg1	heifers1					164:171	heifers1	164:171	heifers1	164:171	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	6	109	theme	collection	1158:1167	arg1	periods					1169:1175	the collection periods	1154:1175	the collection periods	1154:1175	Feces were sampled 4 times a day during the collection periods.					
31325356	2	110	dep	German	471:476	arg1	German					504:509	German	504:509	German	504:509	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	11	111	theme	infused	1853:1859	arg1	starch					1861:1866	infused starch	1853:1866	infused starch	1853:1866	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	10	112	theme	apparent	1554:1561	arg1	digestibility					1563:1575	the apparent digestibility	1550:1575	the apparent digestibility of starch from the total postruminal tract	1550:1618	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	0	113	theme	amounts	53:59	arg1	Effects					0:6	Effects	0:6	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.	0:172	Effects of abomasally infused amylase and increasing amounts of corn starch on fecal excretion of starch, total and microbial nitrogen, and volatile fatty acids in heifers1.					
31325356	2	114	theme	rumen-fistulated	443:458	arg1	Jersey					495:500	Jersey	495:500	Jersey	495:500	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	2	114	theme	rumen-fistulated	443:458	arg1	heifers					460:466	Four rumen-fistulated heifers	438:466	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg	438:554	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	2	114	theme	rumen-fistulated	443:458	arg1	German					471:476	German	471:476	German	471:476	Four rumen-fistulated heifers (2 German Black Pied and 2 Jersey × German Black Pied) with an initial BW of 565 ± 6 kg were fed 5.6 kg DM/d of a diet targeted to contain only a negligible amount of starch.					
31325356	11	115	with	excretion	1785:1793	arg1	feces					1800:1804	feces	1800:1804	feces	1800:1804	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	10	116	theme	total	1596:1600	arg1	tract					1614:1618	the total postruminal tract	1592:1618	the total postruminal tract	1592:1618	In contrast, the apparent digestibility of starch from the total postruminal tract decreased linearly from 90% to 80% (P < 0.001) when the intestinal starch supply increased from 1 to 2 kg/d.					
31325356	1	117	theme	increasing	280:289	arg1	amounts					291:297	increasing amounts	280:297	increasing amounts of corn starch	280:312	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	1	117	theme	increasing	280:289	arg1	starch					307:312	corn starch	302:312	corn starch	302:312	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	13	118	from	factor	2286:2291	arg1	heifers					2328:2334	heifers	2328:2334	heifers	2328:2334	However, amylase did not affect fecal excretion of microbial N or starch, suggesting that pancreatic amylase activity may not be the primarily limiting factor of postruminal starch digestion in heifers when corn starch is abomasally infused in amounts up to 2 kg/d.					
31325356	9	119	theme	Fecal	1346:1350	arg1	excretion					1352:1360	Fecal excretion	1346:1360	Fecal excretion of microbial N	1346:1375	Fecal excretion of microbial N increased linearly with increasing level of starch infusion (P < 0.001), indicating a constant proportion of the infused starch being fermented in the hindgut.					
31325356	3	120	theme	collection	795:804	arg1	d					783:783	25 d	780:783	25 d of sample collection	780:804	Animals were assigned randomly to a crossover trial with 2 experimental periods lasting 35 d each with 10 d of diet adaption followed by 25 d of sample collection.					
31325356	11	121	theme	intestinal	1986:1995	arg1	supply					1997:2002	intestinal supply	1986:2002	intestinal supply	1986:2002	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	8	122	theme	N	1333:1333	arg1	excretion					1335:1343	microbial N excretion	1323:1343	microbial N excretion	1323:1343	Purine bases in feces were determined and used as a marker for microbial N excretion.					
31325356	5	123	theme	g/d	1109:1111	arg1	amounts					1094:1100	amounts	1094:1100	amounts of 724 g/d	1094:1111	At days 6 to 10 the heifers received an abomasal infusion of starch in amounts of 724 g/d.					
31325356	5	123	theme	g/d	1109:1111	arg1	g/d					1109:1111	724 g/d	1105:1111	724 g/d	1105:1111	At days 6 to 10 the heifers received an abomasal infusion of starch in amounts of 724 g/d.					
31325356	11	124	theme	decreasing	1905:1914	arg1	efficiency					1916:1925	a decreasing efficiency	1903:1925	a decreasing efficiency of starch digestion	1903:1945	There is strong evidence based on the increasing starch excretion with feces and the indication of a constant proportion of infused starch being fermented in the hindgut for a decreasing efficiency of starch digestion in the small intestine with increasing intestinal supply.					
31325356	1	125	theme	present	189:195	arg1	study					197:201	the present study	185:201	the present study	185:201	The aim of the present study was to study the effect of exogenous amylase on postruminal disappearance of increasing amounts of corn starch being infused into the abomasum of heifers, and to detect a possible limitation of starch digestion in the small intestine.					
31325356	7	126	theme	fecal	1243:1247	arg1	excretion					1249:1257	fecal excretion	1243:1257	fecal excretion	1243:1257	Titanium dioxide was ruminally administered (10 g/d) to estimate fecal excretion.					
28956533	7	0	theme	NMR	1067:1069	arg1	results					1080:1086	The ATR-FTIR and 13C NMR analysis results	1046:1086	The ATR-FTIR and 13C NMR analysis results	1046:1086	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	6	1	theme	crosslinking	831:842	arg1	phenomenon					844:853	The crosslinking phenomenon	827:853	The crosslinking phenomenon	827:853	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	13	2	from	pH	2053:2054	arg1	way					2046:2048	a controlled way	2033:2048	a controlled way at pH 7 and 7.4	2033:2064	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	1	3	theme	calcium	276:282	arg1	microparticle					258:270	α-tricalcium phosphate (α-TCP) microparticle	227:270	α-tricalcium phosphate (α-TCP) microparticle	227:270	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	3	theme	calcium	276:282	arg1	chloride					284:291	calcium chloride	276:291	calcium chloride	276:291	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	3	theme	calcium	276:282	arg1	alginate					211:218	alginate	211:218	alginate (Alg)	211:224	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	3	theme	calcium	276:282	arg1	biomaterial					325:335	biomaterial	325:335	biomaterial	325:335	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	5	4	dep	strength	728:735	arg1	The					713:715	The	713:715	The	713:715	The mechanical strength and flexibility of the film (Alg-α-TCP) has been controlled by changing its compositions.					
28956533	9	5	link	cross-linked	1578:1589	arg1	network					1591:1597	the cross-linked network	1574:1597	the cross-linked network	1574:1597	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	10	6	theme	film	1662:1665	arg1	stability					1638:1646	thermal stability	1630:1646	thermal stability of the hybrid film	1630:1665	TGA results demonstrated that thermal stability of the hybrid film was enhanced due to ionic crosslinking and interfacial interaction between alginate and α-TCP.					
28956533	3	7	theme	glass	544:548	arg1	slide					550:554	a glass slide	542:554	a glass slide	542:554	For this method, the Alg/α-TCP blend has been prepared using an ultra-sonicator and then put on a glass slide.					
28956533	12	8	theme	cytotoxicity	1892:1903	arg1	tests					1905:1909	cytotoxicity tests	1892:1909	cytotoxicity tests	1892:1909	The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible.					
28956533	4	9	theme	blend	590:594	arg1	volume					580:585	the excess volume	569:585	the excess volume of blend	569:594	After that, the excess volume of blend has been cut off by skidding another slide along with the surface of the blend to achieve proper grade and flatness.					
28956533	13	10	theme	controlled	2035:2044	arg1	way					2046:2048	a controlled way	2033:2048	a controlled way at pH 7 and 7.4	2033:2064	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	13	11	theme	interfacial	2201:2211	arg1	film					2213:2216	an interfacial film	2198:2216	an interfacial film in tissue engineering	2198:2238	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	13	11	theme	interfacial	2201:2211	arg1	°C.					2074:2076	37 °C. Overall	2071:2084	37 °C. Overall	2071:2084	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	12	12	theme	cell	1867:1870	arg1	culture					1880:1886	The in vitro bone cell (MC3T3) culture	1849:1886	The in vitro bone cell (MC3T3) culture	1849:1886	The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible.					
28956533	6	13	theme	reflectance	894:904	arg1	Fourier					906:912	attenuated total reflectance Fourier	877:912	attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses	877:1043	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	12	14	dep	in	1853:1854	arg1	vitro					1856:1860	vitro	1856:1860	vitro	1856:1860	The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible.					
28956533	12	15	theme	MC3T3	1873:1877	arg1	culture					1880:1886	The in vitro bone cell (MC3T3) culture	1849:1886	The in vitro bone cell (MC3T3) culture	1849:1886	The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible.					
28956533	6	16	theme	attenuated	877:886	arg1	Fourier					906:912	attenuated total reflectance Fourier	877:912	attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses	877:1043	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	10	17	theme	interfacial	1710:1720	arg1	interaction					1722:1732	interfacial interaction	1710:1732	interfacial interaction between alginate and α-TCP	1710:1759	TGA results demonstrated that thermal stability of the hybrid film was enhanced due to ionic crosslinking and interfacial interaction between alginate and α-TCP.					
28956533	6	18	theme	nuclear	962:968	arg1	NMR					990:992	NMR	990:992	NMR	990:992	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	6	18	theme	nuclear	962:968	arg1	resonance					979:987	13C nuclear magnetic resonance	958:987	13C nuclear magnetic resonance (NMR)	958:993	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	8	19	theme	hybrid	1383:1388	arg1	films					1390:1394	the Alg-α-TCP hybrid films	1369:1394	the Alg-α-TCP hybrid films	1369:1394	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	8	20	theme	micro-fatigue	1256:1268	arg1	results					1275:1281	The micro-fatigue test results	1252:1281	The micro-fatigue test results	1252:1281	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	0	21	theme	film	88:91	arg1	characterizations					14:30	characterizations	14:30	characterizations	14:30	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	0	21	theme	film	88:91	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	1	22	theme	hybrid	173:178	arg1	film					180:183	A biocompatible hybrid film	157:183	A biocompatible hybrid film	157:183	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	6	23	dep	spectroscopy	933:944	arg1	ATR-FTIR					947:954	ATR-FTIR	947:954	ATR-FTIR	947:954	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	7	24	theme	Ca2+	1168:1171	arg1	ions					1173:1176	Ca2+ ions	1168:1176	Ca2+ ions	1168:1176	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	13	25	theme	serum	1989:1993	arg1	albumin					1995:2001	bovine serum albumin	1982:2001	bovine serum albumin	1982:2001	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	8	26	theme	high	1289:1292	arg1	MPa					1322:1324	up to 257 MPa	1312:1324	up to 257 MPa	1312:1324	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	8	26	theme	high	1289:1292	arg1	strength					1302:1309	high tensile strength	1289:1309	high tensile strength (up to 257 MPa)	1289:1325	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	0	27	theme	high	114:117	arg1	property					130:137	high mechanical property	114:137	high mechanical property	114:137	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	12	28	theme	in	1853:1854	arg1	culture					1880:1886	The in vitro bone cell (MC3T3) culture	1849:1886	The in vitro bone cell (MC3T3) culture	1849:1886	The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible.					
28956533	11	29	theme	hybrid	1836:1841	arg1	film					1843:1846	the hybrid film	1832:1846	the hybrid film	1832:1846	The incorporation of α-TCP particles diminished the swelling ratio of the hybrid film.					
28956533	6	30	dep	Fourier	906:912	arg1	transform					914:922	transform	914:922	transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses	914:1043	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	2	31	theme	finishing	378:386	arg1	process					388:394	a concrete finishing process	367:394	a concrete finishing process	367:394	The 'screeding method' (like a concrete finishing process) has been employed to develop the Alg-α-TCP film.					
28956533	13	32	theme	hybrid	2115:2120	arg1	film					2122:2125	the biocompatible Alg-α-TCP hybrid film	2087:2125	the biocompatible Alg-α-TCP hybrid film	2087:2125	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	13	32	theme	hybrid	2115:2120	arg1	°C.					2074:2076	37 °C. Overall	2071:2084	37 °C. Overall	2071:2084	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	6	33	dep	transform	914:922	arg1	infrared					924:931	infrared	924:931	transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses	914:1043	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	9	34	theme	cross-linked	1578:1589	arg1	network					1591:1597	the cross-linked network	1574:1597	the cross-linked network	1574:1597	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	13	35	from	7.4	2062:2064	arg1	way					2046:2048	a controlled way	2033:2048	a controlled way at pH 7 and 7.4	2033:2064	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	4	36	theme	proper	686:691	arg1	grade					693:697	proper grade	686:697	proper grade	686:697	After that, the excess volume of blend has been cut off by skidding another slide along with the surface of the blend to achieve proper grade and flatness.					
28956533	10	37	theme	TGA	1600:1602	arg1	results					1604:1610	TGA results	1600:1610	TGA results	1600:1610	TGA results demonstrated that thermal stability of the hybrid film was enhanced due to ionic crosslinking and interfacial interaction between alginate and α-TCP.					
28956533	5	38	theme	film	760:763	arg1	strength					728:735	mechanical strength	717:735	mechanical strength	717:735	The mechanical strength and flexibility of the film (Alg-α-TCP) has been controlled by changing its compositions.					
28956533	5	38	theme	film	760:763	arg1	flexibility					741:751	flexibility	741:751	flexibility	741:751	The mechanical strength and flexibility of the film (Alg-α-TCP) has been controlled by changing its compositions.					
28956533	1	39	theme	ionic	301:305	arg1	crosslinking					307:318	ionic crosslinking	301:318	ionic crosslinking	301:318	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	9	40	theme	Alg-α-TCP	1491:1499	arg1	films					1501:1505	Alg-α-TCP films	1491:1505	Alg-α-TCP films	1491:1505	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	13	41	theme	mechanical	2142:2151	arg1	strength					2153:2160	excellent mechanical strength	2132:2160	excellent mechanical strength	2132:2160	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	0	42	theme	alginate-α-tricalcium	35:55	arg1	film					88:91	alginate-α-tricalcium phosphate microparticle hybrid film	35:91	alginate-α-tricalcium phosphate microparticle hybrid film	35:91	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	5	43	theme	mechanical	717:726	arg1	strength					728:735	mechanical strength	717:735	mechanical strength	717:735	The mechanical strength and flexibility of the film (Alg-α-TCP) has been controlled by changing its compositions.					
28956533	7	44	theme	physical	1230:1237	arg1	interaction					1239:1249	physical interaction	1230:1249	physical interaction	1230:1249	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	0	45	with	characterizations	14:30	arg1	flexibility					98:108	flexibility	98:108	flexibility	98:108	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	0	45	with	characterizations	14:30	arg1	property					130:137	high mechanical property	114:137	high mechanical property	114:137	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	11	46	theme	α-TCP	1783:1787	arg1	particles					1789:1797	α-TCP particles	1783:1797	α-TCP particles	1783:1797	The incorporation of α-TCP particles diminished the swelling ratio of the hybrid film.					
28956533	0	47	theme	microparticle	67:79	arg1	film					88:91	alginate-α-tricalcium phosphate microparticle hybrid film	35:91	alginate-α-tricalcium phosphate microparticle hybrid film	35:91	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	8	48	dep	elongation	1354:1363	arg1	%					1352:1352	%	1352:1352	%	1352:1352	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	9	49	theme	cross-sectional	1516:1530	arg1	images					1532:1537	cross-sectional images	1516:1537	cross-sectional images	1516:1537	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	0	50	with	Synthesis	0:8	arg1	flexibility					98:108	flexibility	98:108	flexibility	98:108	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	0	50	with	Synthesis	0:8	arg1	property					130:137	high mechanical property	114:137	high mechanical property	114:137	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	1	51	theme	α-tricalcium	227:238	arg1	microparticle					258:270	α-tricalcium phosphate (α-TCP) microparticle	227:270	α-tricalcium phosphate (α-TCP) microparticle	227:270	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	51	theme	α-tricalcium	227:238	arg1	chloride					284:291	calcium chloride	276:291	calcium chloride	276:291	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	51	theme	α-tricalcium	227:238	arg1	alginate					211:218	alginate	211:218	alginate (Alg)	211:224	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	51	theme	α-tricalcium	227:238	arg1	biomaterial					325:335	biomaterial	325:335	biomaterial	325:335	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	13	52	with	°C.	2074:2076	arg1	flexibility					2166:2176	flexibility	2166:2176	flexibility	2166:2176	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	13	52	with	°C.	2074:2076	arg1	strength					2153:2160	excellent mechanical strength	2132:2160	excellent mechanical strength	2132:2160	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	10	53	theme	hybrid	1655:1660	arg1	film					1662:1665	the hybrid film	1651:1665	the hybrid film	1651:1665	TGA results demonstrated that thermal stability of the hybrid film was enhanced due to ionic crosslinking and interfacial interaction between alginate and α-TCP.					
28956533	0	54	theme	hybrid	81:86	arg1	film					88:91	alginate-α-tricalcium phosphate microparticle hybrid film	35:91	alginate-α-tricalcium phosphate microparticle hybrid film	35:91	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	4	55	theme	excess	573:578	arg1	volume					580:585	the excess volume	569:585	the excess volume of blend	569:594	After that, the excess volume of blend has been cut off by skidding another slide along with the surface of the blend to achieve proper grade and flatness.					
28956533	7	56	theme	13C	1063:1065	arg1	results					1080:1086	The ATR-FTIR and 13C NMR analysis results	1046:1086	The ATR-FTIR and 13C NMR analysis results	1046:1086	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	9	57	theme	α-TCP	1565:1569	arg1	presence					1553:1560	the presence	1549:1560	the presence of α-TCP in the cross-linked network	1549:1597	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	6	58	theme	thermogravimetric	1018:1034	arg1	analyses					1036:1043	thermogravimetric analyses	1018:1043	thermogravimetric analyses	1018:1043	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	7	59	theme	ATR-FTIR	1050:1057	arg1	results					1080:1086	The ATR-FTIR and 13C NMR analysis results	1046:1086	The ATR-FTIR and 13C NMR analysis results	1046:1086	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	10	60	theme	thermal	1630:1636	arg1	stability					1638:1646	thermal stability	1630:1646	thermal stability of the hybrid film	1630:1665	TGA results demonstrated that thermal stability of the hybrid film was enhanced due to ionic crosslinking and interfacial interaction between alginate and α-TCP.					
28956533	8	61	dep	257	1318:1320	arg1	to					1315:1316	to	1315:1316	to	1315:1316	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	13	62	theme	biocompatible	2091:2103	arg1	film					2122:2125	the biocompatible Alg-α-TCP hybrid film	2087:2125	the biocompatible Alg-α-TCP hybrid film	2087:2125	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	13	62	theme	biocompatible	2091:2103	arg1	°C.					2074:2076	37 °C. Overall	2071:2084	37 °C. Overall	2071:2084	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	13	63	theme	tissue	2221:2226	arg1	engineering					2228:2238	tissue engineering	2221:2238	tissue engineering	2221:2238	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	10	64	theme	ionic	1687:1691	arg1	crosslinking					1693:1704	ionic crosslinking	1687:1704	ionic crosslinking	1687:1704	TGA results demonstrated that thermal stability of the hybrid film was enhanced due to ionic crosslinking and interfacial interaction between alginate and α-TCP.					
28956533	2	65	theme	screeding	343:351	arg1	method					353:358	The 'screeding method'	338:359	The 'screeding method' (like a concrete finishing process)	338:395	The 'screeding method' (like a concrete finishing process) has been employed to develop the Alg-α-TCP film.					
28956533	12	66	theme	bone	1862:1865	arg1	culture					1880:1886	The in vitro bone cell (MC3T3) culture	1849:1886	The in vitro bone cell (MC3T3) culture	1849:1886	The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible.					
28956533	6	67	theme	total	888:892	arg1	Fourier					906:912	attenuated total reflectance Fourier	877:912	attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses	877:1043	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	13	68	theme	Alg-α-TCP	2105:2113	arg1	film					2122:2125	the biocompatible Alg-α-TCP hybrid film	2087:2125	the biocompatible Alg-α-TCP hybrid film	2087:2125	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	13	68	theme	Alg-α-TCP	2105:2113	arg1	°C.					2074:2076	37 °C. Overall	2071:2084	37 °C. Overall	2071:2084	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	9	69	attach	presence	1553:1560	arg1	network					1591:1597	the cross-linked network	1574:1597	the cross-linked network	1574:1597	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	9	69	attach	presence	1553:1560	arg2	α-TCP					1565:1569	α-TCP	1565:1569	α-TCP	1565:1569	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	8	70	dep	13	1350:1351	arg1	to					1347:1348	to	1347:1348	to	1347:1348	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	8	71	theme	Alg-α-TCP	1373:1381	arg1	films					1390:1394	the Alg-α-TCP hybrid films	1369:1394	the Alg-α-TCP hybrid films	1369:1394	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	9	72	theme	SEM	1401:1403	arg1	analysis					1405:1412	The SEM analysis	1397:1412	The SEM analysis	1397:1412	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	6	73	theme	magnetic	970:977	arg1	NMR					990:992	NMR	990:992	NMR	990:992	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	6	73	theme	magnetic	970:977	arg1	resonance					979:987	13C nuclear magnetic resonance	958:987	13C nuclear magnetic resonance (NMR)	958:993	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	8	74	theme	films	1390:1394	arg1	MPa					1322:1324	up to 257 MPa	1312:1324	up to 257 MPa	1312:1324	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	8	74	theme	films	1390:1394	arg1	strength					1302:1309	high tensile strength	1289:1309	high tensile strength (up to 257 MPa)	1289:1325	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	8	74	theme	films	1390:1394	arg1	flexibility					1331:1341	flexibility	1331:1341	flexibility (up to 13% elongation)	1331:1364	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	8	74	theme	films	1390:1394	arg1	elongation					1354:1363	up to 13% elongation	1344:1363	up to 13% elongation	1344:1363	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	1	75	theme	biocompatible	159:171	arg1	film					180:183	A biocompatible hybrid film	157:183	A biocompatible hybrid film	157:183	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	6	76	theme	13C	958:960	arg1	NMR					990:992	NMR	990:992	NMR	990:992	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	6	76	theme	13C	958:960	arg1	resonance					979:987	13C nuclear magnetic resonance	958:987	13C nuclear magnetic resonance (NMR)	958:993	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	13	77	theme	bovine	1982:1987	arg1	albumin					1995:2001	bovine serum albumin	1982:2001	bovine serum albumin	1982:2001	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	9	78	theme	α-TCP	1427:1431	arg1	particles					1433:1441	the α-TCP particles	1423:1441	the α-TCP particles	1423:1441	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	8	79	theme	test	1270:1273	arg1	results					1275:1281	The micro-fatigue test results	1252:1281	The micro-fatigue test results	1252:1281	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	0	80	theme	mechanical	119:128	arg1	property					130:137	high mechanical property	114:137	high mechanical property	114:137	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	7	81	theme	alginate	1127:1134	arg1	groups					1113:1118	carboxylate groups	1101:1118	carboxylate groups of the alginate	1101:1134	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	7	81	theme	alginate	1127:1134	arg1	alginate					1127:1134	the alginate	1123:1134	the alginate	1123:1134	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	9	82	from	presence	1553:1560	arg1	network					1591:1597	the cross-linked network	1574:1597	the cross-linked network	1574:1597	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	8	83	theme	tensile	1294:1300	arg1	MPa					1322:1324	up to 257 MPa	1312:1324	up to 257 MPa	1312:1324	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	8	83	theme	tensile	1294:1300	arg1	strength					1302:1309	high tensile strength	1289:1309	high tensile strength (up to 257 MPa)	1289:1325	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	3	84	theme	Alg/α-TCP	467:475	arg1	blend					477:481	the Alg/α-TCP blend	463:481	the Alg/α-TCP blend	463:481	For this method, the Alg/α-TCP blend has been prepared using an ultra-sonicator and then put on a glass slide.					
28956533	11	85	theme	film	1843:1846	arg1	ratio					1823:1827	the swelling ratio	1810:1827	the swelling ratio of the hybrid film	1810:1846	The incorporation of α-TCP particles diminished the swelling ratio of the hybrid film.					
28956533	6	86	theme	x-ray	996:1000	arg1	diffraction					1002:1012	x-ray diffraction	996:1012	x-ray diffraction	996:1012	The crosslinking phenomenon has been confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), 13C nuclear magnetic resonance (NMR), x-ray diffraction and thermogravimetric analyses.					
28956533	2	87	theme	Alg-α-TCP	430:438	arg1	film					440:443	the Alg-α-TCP film	426:443	the Alg-α-TCP film	426:443	The 'screeding method' (like a concrete finishing process) has been employed to develop the Alg-α-TCP film.					
28956533	4	88	theme	blend	669:673	arg1	surface					654:660	the surface	650:660	the surface of the blend to achieve proper grade and flatness	650:710	After that, the excess volume of blend has been cut off by skidding another slide along with the surface of the blend to achieve proper grade and flatness.					
28956533	2	89	theme	concrete	369:376	arg1	process					388:394	a concrete finishing process	367:394	a concrete finishing process	367:394	The 'screeding method' (like a concrete finishing process) has been employed to develop the Alg-α-TCP film.					
28956533	7	90	theme	carboxylate	1101:1111	arg1	groups					1113:1118	carboxylate groups	1101:1118	carboxylate groups of the alginate	1101:1134	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	7	90	theme	carboxylate	1101:1111	arg1	alginate					1127:1134	the alginate	1123:1134	the alginate	1123:1134	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	11	91	theme	swelling	1814:1821	arg1	ratio					1823:1827	the swelling ratio	1810:1827	the swelling ratio of the hybrid film	1810:1846	The incorporation of α-TCP particles diminished the swelling ratio of the hybrid film.					
28956533	0	92	theme	phosphate	57:65	arg1	film					88:91	alginate-α-tricalcium phosphate microparticle hybrid film	35:91	alginate-α-tricalcium phosphate microparticle hybrid film	35:91	Synthesis and characterizations of alginate-α-tricalcium phosphate microparticle hybrid film with flexibility and high mechanical property as a biomaterial.					
28956533	13	93	theme	excellent	2132:2140	arg1	strength					2153:2160	excellent mechanical strength	2132:2160	excellent mechanical strength	2132:2160	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	9	94	theme	films	1501:1505	arg1	surface					1480:1486	the surface	1476:1486	the surface of Alg-α-TCP films	1476:1505	The SEM analysis suggests the α-TCP particles are homogeneously distributed on the surface of Alg-α-TCP films, whereas cross-sectional images confirmed the presence of α-TCP in the cross-linked network.					
28956533	13	95	from	film	2213:2216	arg1	engineering					2228:2238	tissue engineering	2221:2238	tissue engineering	2221:2238	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	7	96	theme	analysis	1071:1078	arg1	results					1080:1086	The ATR-FTIR and 13C NMR analysis results	1046:1086	The ATR-FTIR and 13C NMR analysis results	1046:1086	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	12	97	theme	hybrid	1927:1932	arg1	film					1934:1937	the hybrid film	1923:1937	the hybrid film	1923:1937	The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible.					
28956533	12	97	theme	hybrid	1927:1932	arg1	biocompatible					1942:1954	biocompatible	1942:1954	biocompatible	1942:1954	The in vitro bone cell (MC3T3) culture and cytotoxicity tests showed that the hybrid film is biocompatible.					
28956533	13	98	theme	hybrid	1961:1966	arg1	film					1968:1971	The hybrid film	1957:1971	The hybrid film	1957:1971	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	11	99	theme	particles	1789:1797	arg1	incorporation					1766:1778	The incorporation	1762:1778	The incorporation of α-TCP particles	1762:1797	The incorporation of α-TCP particles diminished the swelling ratio of the hybrid film.					
28956533	8	100	dep	%	1352:1352	arg1	13					1350:1351	13	1350:1351	13	1350:1351	The micro-fatigue test results imply high tensile strength (up to 257 MPa) and flexibility (up to 13% elongation) of the Alg-α-TCP hybrid films.					
28956533	1	101	theme	phosphate	240:248	arg1	microparticle					258:270	α-tricalcium phosphate (α-TCP) microparticle	227:270	α-tricalcium phosphate (α-TCP) microparticle	227:270	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	101	theme	phosphate	240:248	arg1	chloride					284:291	calcium chloride	276:291	calcium chloride	276:291	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	101	theme	phosphate	240:248	arg1	alginate					211:218	alginate	211:218	alginate (Alg)	211:224	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	101	theme	phosphate	240:248	arg1	biomaterial					325:335	biomaterial	325:335	biomaterial	325:335	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	7	102	theme	α-TCP	1189:1193	arg1	particles					1195:1203	the α-TCP particles	1185:1203	the α-TCP particles	1185:1203	The ATR-FTIR and 13C NMR analysis results suggest that carboxylate groups of the alginate are ionically cross-linked with Ca2+ ions, while the α-TCP particles reside in the network by physical interaction.					
28956533	13	103	theme	Overall	2078:2084	arg1	film					2213:2216	an interfacial film	2198:2216	an interfacial film in tissue engineering	2198:2238	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	13	103	theme	Overall	2078:2084	arg1	film					2122:2125	the biocompatible Alg-α-TCP hybrid film	2087:2125	the biocompatible Alg-α-TCP hybrid film	2087:2125	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	13	103	theme	Overall	2078:2084	arg1	°C.					2074:2076	37 °C. Overall	2071:2084	37 °C. Overall	2071:2084	The hybrid film releases bovine serum albumin and dimethyloxaloylglycine in a controlled way at pH 7 and 7.4, and 37 °C. Overall, the biocompatible Alg-α-TCP hybrid film with excellent mechanical strength and flexibility could be applied as an interfacial film in tissue engineering.					
28956533	1	104	theme	α-TCP	251:255	arg1	microparticle					258:270	α-tricalcium phosphate (α-TCP) microparticle	227:270	α-tricalcium phosphate (α-TCP) microparticle	227:270	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	104	theme	α-TCP	251:255	arg1	chloride					284:291	calcium chloride	276:291	calcium chloride	276:291	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	104	theme	α-TCP	251:255	arg1	alginate					211:218	alginate	211:218	alginate (Alg)	211:224	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
28956533	1	104	theme	α-TCP	251:255	arg1	biomaterial					325:335	biomaterial	325:335	biomaterial	325:335	A biocompatible hybrid film has been fabricated using alginate (Alg), α-tricalcium phosphate (α-TCP) microparticle and calcium chloride through ionic crosslinking as a biomaterial.					
29584947	0	0	from	Embedded	20:27	arg1	Films					60:64	Cellulose Nanocrystal Based Films	32:64	Cellulose Nanocrystal Based Films	32:64	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	7	1	theme	bionanocomposite	1090:1105	arg1	films					1107:1111	The bionanocomposite films	1086:1111	The bionanocomposite films based on the metal nanoparticles	1086:1144	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	2	2	theme	free-standing	377:389	arg1	films					405:409	free-standing nanocomposite films	377:409	free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles	377:504	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	1	3	theme	multifunctional	260:274	arg1	materials					276:284	multifunctional materials	260:284	multifunctional materials	260:284	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	5	4	theme	first	948:952	arg1	time					954:957	the first time	944:957	the first time	944:957	Furthermore, disintegrability in composting condition of CNC based nanocomposites was here investigated for the first time.					
29584947	1	5	theme	shape-	166:171	arg1	properties					200:209	different size-, shape-, and composition-dependent properties	149:209	different size-, shape-, and composition-dependent properties	149:209	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	6	6	theme	structure	1034:1042	arg1	formation					1004:1012	the formation	1000:1012	the formation of a chiral nematic structure with uniformly distributed nanoparticles	1000:1083	The morphological observations revealed the formation of a chiral nematic structure with uniformly distributed nanoparticles.					
29584947	8	7	theme	advantageous	1443:1454	arg1	characteristics					1456:1470	highly advantageous characteristics	1436:1470	highly advantageous characteristics that can for the new development of multifunctional materials	1436:1532	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	4	8	theme	antimicrobial	777:789	arg1	assays					791:796	antimicrobial assays	777:796	antimicrobial assays	777:796	Morphological and optical characteristics, chemical properties, wettability, and antimicrobial assays of the produced films were conducted.					
29584947	8	9	from	nature	1396:1401	arg1	world					1367:1371	the world	1363:1371	the world	1363:1371	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	6	10	theme	nematic	1026:1032	arg1	structure					1034:1042	a chiral nematic structure	1017:1042	a chiral nematic structure	1017:1042	The morphological observations revealed the formation of a chiral nematic structure with uniformly distributed nanoparticles.					
29584947	0	11	theme	Post-use	91:98	arg1	Analysis					100:107	Post-use Analysis	91:107	Post-use Analysis	91:107	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	0	12	from	Films	60:64	arg1	Embedded					20:27	Embedded	20:27	Embedded	20:27	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	4	13	theme	chemical	739:746	arg1	properties					748:757	chemical properties	739:757	chemical properties	739:757	Morphological and optical characteristics, chemical properties, wettability, and antimicrobial assays of the produced films were conducted.					
29584947	6	14	theme	chiral	1019:1024	arg1	structure					1034:1042	a chiral nematic structure	1017:1042	a chiral nematic structure	1017:1042	The morphological observations revealed the formation of a chiral nematic structure with uniformly distributed nanoparticles.					
29584947	3	15	from	self-assembly	653:665	arg1	solution					686:693	acqueous solution	677:693	acqueous solution	677:693	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	8	16	theme	large	1332:1336	arg1	quantity					1338:1345	the large quantity	1328:1345	the large quantity of cellulose in the world	1328:1371	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	7	17	theme	antimicrobial	1160:1172	arg1	activity					1174:1181	effective antimicrobial activity	1150:1181	effective antimicrobial activity	1150:1181	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	8	18	theme	free-standing	1382:1394	arg1	nature					1396:1401	the free-standing nature	1378:1401	the free-standing nature of the nanocomposite films	1378:1428	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	5	19	theme	based	897:901	arg1	nanocomposites					903:916	CNC based nanocomposites	893:916	CNC based nanocomposites	893:916	Furthermore, disintegrability in composting condition of CNC based nanocomposites was here investigated for the first time.					
29584947	8	20	from	world	1367:1371	arg1	quantity					1338:1345	the large quantity	1328:1345	the large quantity of cellulose in the world	1328:1371	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	20	from	world	1367:1371	arg1	method					1300:1305	The simplicity method	1285:1305	The simplicity method of film preparation	1285:1325	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	20	from	world	1367:1371	arg1	nature					1396:1401	the free-standing nature	1378:1401	the free-standing nature of the nanocomposite films	1378:1428	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	2	21	theme	different	467:475	arg1	types					477:481	three different types	461:481	three different types of metal nanoparticles	461:504	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	2	22	theme	cellulose	420:428	arg1	CNC					444:446	CNC	444:446	CNC	444:446	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	2	22	theme	cellulose	420:428	arg1	nanocrystals					430:441	cellulose nanocrystals	420:441	cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles	420:504	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	3	23	contain	having	532:537	arg2	oxide					544:548	zinc oxide	539:548	zinc oxide (ZnO)	539:554	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	23	contain	having	532:537	arg1	nanocomposites					517:530	CNC-based nanocomposites	507:530	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O)	507:604	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	23	contain	having	532:537	arg2	oxide					593:597	silver oxide	586:597	silver oxide (Ag2O)	586:604	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	23	contain	having	532:537	arg2	dioxide					566:572	titanium dioxide	557:572	titanium dioxide (TiO2)	557:579	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	23	contain	having	532:537	arg2	ZnO					551:553	ZnO	551:553	ZnO	551:553	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	23	contain	having	532:537	arg2	Ag2O					600:603	Ag2O	600:603	Ag2O	600:603	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	23	contain	having	532:537	arg2	TiO2					575:578	TiO2	575:578	TiO2	575:578	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	2	24	dep	preparation	347:357	arg1	the					343:345	the	343:345	the	343:345	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	7	25	dep	aureus	1250:1255	arg1	8325-4					1257:1262	8325-4	1257:1262	8325-4	1257:1262	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	7	25	dep	aureus	1250:1255	arg1	aureus					1269:1274	S. aureus	1266:1274	S. aureus 8325-4	1266:1281	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	1	26	theme	composition-dependent	178:198	arg1	properties					200:209	different size-, shape-, and composition-dependent properties	149:209	different size-, shape-, and composition-dependent properties	149:209	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	0	27	theme	Metal	0:4	arg1	Nanoparticles					6:18	Metal Nanoparticles	0:18	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.	0:108	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	7	28	theme	E.	1219:1220	arg1	RB					1227:1228	E. coli RB	1219:1228	E. coli RB	1219:1228	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	7	28	theme	E.	1219:1220	arg1	coli					1209:1212	Escherichia coli	1197:1212	Escherichia coli RB ( E. coli RB)	1197:1229	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	7	29	theme	effective	1150:1158	arg1	activity					1174:1181	effective antimicrobial activity	1150:1181	effective antimicrobial activity	1150:1181	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	7	30	dep	coli	1209:1212	arg1	RB					1214:1215	RB	1214:1215	Escherichia coli RB ( E. coli RB)	1197:1229	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	5	31	theme	nanocomposites	903:916	arg1	condition					880:888	composting condition	869:888	composting condition of CNC based nanocomposites	869:916	Furthermore, disintegrability in composting condition of CNC based nanocomposites was here investigated for the first time.					
29584947	7	32	theme	metal	1126:1130	arg1	nanoparticles					1132:1144	the metal nanoparticles	1122:1144	the metal nanoparticles	1122:1144	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	8	33	from	cellulose	1350:1358	arg1	world					1367:1371	the world	1363:1371	the world	1363:1371	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	2	34	theme	detailed	317:324	arg1	investigation					326:338	a detailed investigation	315:338	a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles	315:504	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	8	35	theme	film	1310:1313	arg1	preparation					1315:1325	film preparation	1310:1325	film preparation	1310:1325	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	0	36	theme	Embedded	20:27	arg1	Nanoparticles					6:18	Metal Nanoparticles	0:18	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.	0:108	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	7	37	dep	aureus	1269:1274	arg1	8325-4					1276:1281	8325-4	1276:1281	8325-4	1276:1281	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	8	38	theme	new	1489:1491	arg1	development					1493:1503	the new development	1485:1503	the new development of multifunctional materials	1485:1532	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	4	39	theme	Morphological	696:708	arg1	characteristics					722:736	Morphological and optical characteristics	696:736	Morphological and optical characteristics	696:736	Morphological and optical characteristics, chemical properties, wettability, and antimicrobial assays of the produced films were conducted.					
29584947	2	40	theme	films	405:409	arg1	properties					363:372	properties	363:372	properties	363:372	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	2	40	theme	films	405:409	arg1	preparation					347:357	preparation	347:357	preparation	347:357	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	0	41	theme	Nanocrystal	42:52	arg1	Films					60:64	Cellulose Nanocrystal Based Films	32:64	Cellulose Nanocrystal Based Films	32:64	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	4	42	theme	optical	714:720	arg1	characteristics					722:736	Morphological and optical characteristics	696:736	Morphological and optical characteristics	696:736	Morphological and optical characteristics, chemical properties, wettability, and antimicrobial assays of the produced films were conducted.					
29584947	5	43	theme	composting	869:878	arg1	condition					880:888	composting condition	869:888	composting condition of CNC based nanocomposites	869:916	Furthermore, disintegrability in composting condition of CNC based nanocomposites was here investigated for the first time.					
29584947	8	44	theme	simplicity	1289:1298	arg1	method					1300:1305	The simplicity method	1285:1305	The simplicity method of film preparation	1285:1325	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	1	45	theme	exciting	217:224	arg1	dispersion					114:123	The dispersion	110:123	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties	110:209	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	1	45	theme	exciting	217:224	arg1	approach					226:233	an exciting approach	214:233	an exciting approach to design	214:243	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	0	46	theme	Cellulose	32:40	arg1	Films					60:64	Cellulose Nanocrystal Based Films	32:64	Cellulose Nanocrystal Based Films	32:64	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	2	47	theme	nanoparticles	492:504	arg1	types					477:481	three different types	461:481	three different types of metal nanoparticles	461:504	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	8	48	theme	cellulose	1350:1358	arg1	quantity					1338:1345	the large quantity	1328:1345	the large quantity of cellulose in the world	1328:1371	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	48	theme	cellulose	1350:1358	arg1	method					1300:1305	The simplicity method	1285:1305	The simplicity method of film preparation	1285:1325	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	48	theme	cellulose	1350:1358	arg1	nature					1396:1401	the free-standing nature	1378:1401	the free-standing nature of the nanocomposite films	1378:1428	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	49	theme	films	1424:1428	arg1	quantity					1338:1345	the large quantity	1328:1345	the large quantity of cellulose in the world	1328:1371	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	49	theme	films	1424:1428	arg1	method					1300:1305	The simplicity method	1285:1305	The simplicity method of film preparation	1285:1325	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	49	theme	films	1424:1428	arg1	nature					1396:1401	the free-standing nature	1378:1401	the free-standing nature of the nanocomposite films	1378:1428	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	50	from	method	1300:1305	arg1	world					1367:1371	the world	1363:1371	the world	1363:1371	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	51	theme	preparation	1315:1325	arg1	quantity					1338:1345	the large quantity	1328:1345	the large quantity of cellulose in the world	1328:1371	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	51	theme	preparation	1315:1325	arg1	method					1300:1305	The simplicity method	1285:1305	The simplicity method of film preparation	1285:1325	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	51	theme	preparation	1315:1325	arg1	nature					1396:1401	the free-standing nature	1378:1401	the free-standing nature of the nanocomposite films	1378:1428	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	2	52	theme	metal	486:490	arg1	nanoparticles					492:504	metal nanoparticles	486:504	metal nanoparticles	486:504	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	3	53	theme	evaporation-induced	633:651	arg1	self-assembly					653:665	evaporation-induced self-assembly	633:665	evaporation-induced self-assembly (EISA) in acqueous solution	633:693	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	53	theme	evaporation-induced	633:651	arg1	EISA					668:671	EISA	668:671	EISA	668:671	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	54	theme	silver	586:591	arg1	Ag2O					600:603	Ag2O	600:603	Ag2O	600:603	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	54	theme	silver	586:591	arg1	oxide					593:597	silver oxide	586:597	silver oxide (Ag2O)	586:604	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	2	55	theme	properties	363:372	arg1	investigation					326:338	a detailed investigation	315:338	a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles	315:504	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	0	56	theme	Based	54:58	arg1	Films					60:64	Cellulose Nanocrystal Based Films	32:64	Cellulose Nanocrystal Based Films	32:64	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	6	57	theme	morphological	964:976	arg1	observations					978:989	The morphological observations	960:989	The morphological observations	960:989	The morphological observations revealed the formation of a chiral nematic structure with uniformly distributed nanoparticles.					
29584947	3	58	theme	CNC-based	507:515	arg1	nanocomposites					517:530	CNC-based nanocomposites	507:530	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O)	507:604	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	8	59	theme	materials	1524:1532	arg1	development					1493:1503	the new development	1485:1503	the new development of multifunctional materials	1485:1532	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	8	60	theme	multifunctional	1508:1522	arg1	materials					1524:1532	multifunctional materials	1508:1532	multifunctional materials	1508:1532	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	5	61	from	disintegrability	849:864	arg1	condition					880:888	composting condition	869:888	composting condition of CNC based nanocomposites	869:916	Furthermore, disintegrability in composting condition of CNC based nanocomposites was here investigated for the first time.					
29584947	3	62	theme	zinc	539:542	arg1	ZnO					551:553	ZnO	551:553	ZnO	551:553	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	62	theme	zinc	539:542	arg1	oxide					544:548	zinc oxide	539:548	zinc oxide (ZnO)	539:554	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	63	theme	acqueous	677:684	arg1	solution					686:693	acqueous solution	677:693	acqueous solution	677:693	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	4	64	theme	produced	805:812	arg1	films					814:818	the produced films	801:818	the produced films	801:818	Morphological and optical characteristics, chemical properties, wettability, and antimicrobial assays of the produced films were conducted.					
29584947	8	65	from	quantity	1338:1345	arg1	world					1367:1371	the world	1363:1371	the world	1363:1371	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	1	66	theme	nanoparticles	128:140	arg1	dispersion					114:123	The dispersion	110:123	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties	110:209	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	1	66	theme	nanoparticles	128:140	arg1	approach					226:233	an exciting approach	214:233	an exciting approach to design	214:243	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	7	67	contain	had	1146:1148	arg1	films					1107:1111	The bionanocomposite films	1086:1111	The bionanocomposite films based on the metal nanoparticles	1086:1144	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	7	67	contain	had	1146:1148	arg2	activity					1174:1181	effective antimicrobial activity	1150:1181	effective antimicrobial activity	1150:1181	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
29584947	3	68	theme	titanium	557:564	arg1	dioxide					566:572	titanium dioxide	557:572	titanium dioxide (TiO2)	557:579	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	3	68	theme	titanium	557:564	arg1	TiO2					575:578	TiO2	575:578	TiO2	575:578	CNC-based nanocomposites having zinc oxide (ZnO), titanium dioxide (TiO2), and silver oxide (Ag2O) have been obtained through evaporation-induced self-assembly (EISA) in acqueous solution.					
29584947	6	69	with	formation	1004:1012	arg1	nanoparticles					1071:1083	uniformly distributed nanoparticles	1049:1083	uniformly distributed nanoparticles	1049:1083	The morphological observations revealed the formation of a chiral nematic structure with uniformly distributed nanoparticles.					
29584947	1	70	theme	size-	159:163	arg1	properties					200:209	different size-, shape-, and composition-dependent properties	149:209	different size-, shape-, and composition-dependent properties	149:209	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	2	71	theme	preparation	347:357	arg1	investigation					326:338	a detailed investigation	315:338	a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles	315:504	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	0	72	theme	Material	67:74	arg1	Properties					76:85	Material Properties	67:85	Material Properties	67:85	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	8	73	theme	nanocomposite	1410:1422	arg1	films					1424:1428	the nanocomposite films	1406:1428	the nanocomposite films	1406:1428	The simplicity method of film preparation, the large quantity of cellulose in the world, and the free-standing nature of the nanocomposite films offer highly advantageous characteristics that can for the new development of multifunctional materials.					
29584947	0	74	dep	Nanoparticles	6:18	arg1	Properties					76:85	Material Properties	67:85	Material Properties	67:85	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	0	74	dep	Nanoparticles	6:18	arg1	Analysis					100:107	Post-use Analysis	91:107	Post-use Analysis	91:107	Metal Nanoparticles Embedded in Cellulose Nanocrystal Based Films: Material Properties and Post-use Analysis.					
29584947	1	75	theme	different	149:157	arg1	properties					200:209	different size-, shape-, and composition-dependent properties	149:209	different size-, shape-, and composition-dependent properties	149:209	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	5	76	theme	CNC	893:895	arg1	nanocomposites					903:916	CNC based nanocomposites	893:916	CNC based nanocomposites	893:916	Furthermore, disintegrability in composting condition of CNC based nanocomposites was here investigated for the first time.					
29584947	6	77	theme	distributed	1059:1069	arg1	nanoparticles					1071:1083	uniformly distributed nanoparticles	1049:1083	uniformly distributed nanoparticles	1049:1083	The morphological observations revealed the formation of a chiral nematic structure with uniformly distributed nanoparticles.					
29584947	1	78	contain	having	142:147	arg1	nanoparticles					128:140	nanoparticles	128:140	nanoparticles having different size-, shape-, and composition-dependent properties	128:209	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	1	78	contain	having	142:147	arg2	properties					200:209	different size-, shape-, and composition-dependent properties	149:209	different size-, shape-, and composition-dependent properties	149:209	The dispersion of nanoparticles having different size-, shape-, and composition-dependent properties is an exciting approach to design and synthesize multifunctional materials and devices.					
29584947	4	79	theme	films	814:818	arg1	wettability					760:770	wettability	760:770	wettability	760:770	Morphological and optical characteristics, chemical properties, wettability, and antimicrobial assays of the produced films were conducted.					
29584947	4	79	theme	films	814:818	arg1	characteristics					722:736	Morphological and optical characteristics	696:736	Morphological and optical characteristics	696:736	Morphological and optical characteristics, chemical properties, wettability, and antimicrobial assays of the produced films were conducted.					
29584947	4	79	theme	films	814:818	arg1	properties					748:757	chemical properties	739:757	chemical properties	739:757	Morphological and optical characteristics, chemical properties, wettability, and antimicrobial assays of the produced films were conducted.					
29584947	4	79	theme	films	814:818	arg1	assays					791:796	antimicrobial assays	777:796	antimicrobial assays	777:796	Morphological and optical characteristics, chemical properties, wettability, and antimicrobial assays of the produced films were conducted.					
29584947	2	80	theme	nanocomposite	391:403	arg1	films					405:409	free-standing nanocomposite films	377:409	free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles	377:504	This work shows a detailed investigation of the preparation and properties of free-standing nanocomposite films based on cellulose nanocrystals (CNC) loaded with three different types of metal nanoparticles.					
29584947	7	81	dep	E.	1219:1220	arg1	coli					1222:1225	coli	1222:1225	coli	1222:1225	The bionanocomposite films based on the metal nanoparticles had effective antimicrobial activity, killing both Escherichia coli RB ( E. coli RB) and Staphylococcus aureus 8325-4 ( S. aureus 8325-4).					
31195757	0	0	theme	Preterm	74:80	arg1	Infants					82:88	Term versus Preterm Infants	62:88	Infants	82:88	Human Milk Oligosaccharides in the Milk of Mothers Delivering Term versus Preterm Infants.					
31195757	0	1	from	Oligosaccharides	11:26	arg1	Milk					35:38	the Milk	31:38	the Milk of Mothers	31:49	Human Milk Oligosaccharides in the Milk of Mothers Delivering Term versus Preterm Infants.					
31195757	4	2	from	samples	533:539	arg1	mothers					557:563	25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants	554:696	mothers	557:563	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	4	2	from	samples	533:539	arg1	mothers					663:669	28 mothers	660:669	28 mothers	660:669	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	5	3	from	age	772:774	arg1	comparable					736:745	comparable	736:745	comparable	736:745	The concentrations of most HMOs were comparable at equivalent postpartum age.					
31195757	5	3	from	age	772:774	arg1	concentrations					703:716	The concentrations	699:716	The concentrations of most HMOs	699:729	The concentrations of most HMOs were comparable at equivalent postpartum age.					
31195757	1	4	theme	major	132:136	arg1	oligosaccharides					102:117	Human milk oligosaccharides	91:117	Human milk oligosaccharides (HMOs)	91:124	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	1	4	theme	major	132:136	arg1	component					138:146	a major component	130:146	a major component of human milk	130:160	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	8	5	from	different	1156:1164	arg1	preterm					1169:1175	preterm	1169:1175	preterm	1169:1175	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	10	6	theme	further	1379:1385	arg1	investigation					1387:1399	further investigation	1379:1399	further investigation	1379:1399	The observed differences warrant further investigation in view of their potential clinical impact.					
31195757	7	7	theme	preterm	990:996	arg1	milk					998:1001	preterm milk	990:1001	preterm milk	990:1001	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	10	8	theme	observed	1350:1357	arg1	differences					1359:1369	The observed differences	1346:1369	The observed differences	1346:1369	The observed differences warrant further investigation in view of their potential clinical impact.					
31195757	7	9	from	different	977:985	arg1	milk					998:1001	preterm milk	990:1001	preterm milk	990:1001	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	4	10	dep	infants	592:598	arg1	weeks					606:610	< 32 weeks	601:610	< 32 weeks of gestational age	601:629	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	4	10	dep	infants	592:598	arg1	1500g					634:638	< 1500g	632:638	< 1500g of birthweight	632:653	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	7	11	theme	3'-sialyllactose	1018:1033	arg1	concentrations					1035:1048	particular 3'-sialyllactose concentrations	1007:1048	particular 3'-sialyllactose concentrations	1007:1048	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	4	12	from	mothers	663:669	arg1	samples					533:539	500 samples	529:539	500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants	529:696	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	4	12	from	mothers	663:669	arg1	milk					544:547	milk	544:547	milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants	544:696	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	4	13	theme	birthweight	643:653	arg1	weeks					606:610	< 32 weeks	601:610	< 32 weeks of gestational age	601:629	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	4	13	theme	birthweight	643:653	arg1	1500g					634:638	< 1500g	632:638	< 1500g of birthweight	632:653	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	6	14	theme	lactation	894:902	arg1	month					885:889	the first month	875:889	the first month of lactation	875:902	However, HMOs containing α-1,2-linked fucose were reduced in concentration in preterm milk during the first month of lactation.					
31195757	6	15	theme	α-1,2-linked	802:813	arg1	fucose					815:820	α-1,2-linked fucose	802:820	α-1,2-linked fucose	802:820	However, HMOs containing α-1,2-linked fucose were reduced in concentration in preterm milk during the first month of lactation.					
31195757	7	16	theme	number	929:934	arg1	different					977:985	different	977:985	different	977:985	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	7	16	theme	number	929:934	arg1	concentrations					909:922	The concentrations	905:922	The concentrations of a number of sialylated oligosaccharides	905:965	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	2	17	theme	human	316:320	arg1	milk					322:325	human milk	316:325	human milk	316:325	Preterm infants are particularly vulnerable, but have improved outcomes if fed with human milk.					
31195757	5	18	theme	postpartum	761:770	arg1	age					772:774	equivalent postpartum age	750:774	equivalent postpartum age	750:774	The concentrations of most HMOs were comparable at equivalent postpartum age.					
31195757	9	19	theme	highest	1314:1320	arg1	concentrations					1322:1335	the highest concentrations	1310:1335	the highest concentrations of HMOs	1310:1343	The largest differences manifest around 40 weeks of postmenstrual age, when the milk of term infants contains the highest concentrations of HMOs.					
31195757	9	20	contain	contains	1301:1308	arg2	concentrations					1322:1335	the highest concentrations	1310:1335	the highest concentrations of HMOs	1310:1343	The largest differences manifest around 40 weeks of postmenstrual age, when the milk of term infants contains the highest concentrations of HMOs.					
31195757	9	20	contain	contains	1301:1308	arg1	milk					1280:1283	the milk	1276:1283	the milk of term infants	1276:1299	The largest differences manifest around 40 weeks of postmenstrual age, when the milk of term infants contains the highest concentrations of HMOs.					
31195757	3	21	theme	equivalent	466:475	arg1	age					491:493	equivalent postmenstrual age	466:493	equivalent postmenstrual age	466:493	This study aimed to determine if the HMO composition of preterm milk differed from that of term milk at equivalent stage of lactation and equivalent postmenstrual age.					
31195757	3	22	theme	preterm	384:390	arg1	milk					392:395	preterm milk	384:395	preterm milk	384:395	This study aimed to determine if the HMO composition of preterm milk differed from that of term milk at equivalent stage of lactation and equivalent postmenstrual age.					
31195757	7	23	theme	particular	1007:1016	arg1	concentrations					1035:1048	particular 3'-sialyllactose concentrations	1007:1048	particular 3'-sialyllactose concentrations	1007:1048	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	2	24	theme	Preterm	232:238	arg1	infants					240:246	Preterm infants	232:246	Preterm infants	232:246	Preterm infants are particularly vulnerable, but have improved outcomes if fed with human milk.					
31195757	1	25	theme	human	151:155	arg1	milk					157:160	human milk	151:160	human milk	151:160	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	0	26	theme	Milk	6:9	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides in the Milk of Mothers	0:49	Human Milk Oligosaccharides in the Milk of Mothers Delivering Term versus Preterm Infants.					
31195757	7	27	theme	sialylated	939:948	arg1	oligosaccharides					950:965	sialylated oligosaccharides	939:965	sialylated oligosaccharides	939:965	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	9	28	theme	age	1266:1268	arg1	weeks					1243:1247	40 weeks	1240:1247	40 weeks of postmenstrual age	1240:1268	The largest differences manifest around 40 weeks of postmenstrual age, when the milk of term infants contains the highest concentrations of HMOs.					
31195757	1	29	theme	milk	157:160	arg1	oligosaccharides					102:117	Human milk oligosaccharides	91:117	Human milk oligosaccharides (HMOs)	91:124	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	1	29	theme	milk	157:160	arg1	component					138:146	a major component	130:146	a major component of human milk	130:160	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	0	30	theme	Human	0:4	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides in the Milk of Mothers	0:49	Human Milk Oligosaccharides in the Milk of Mothers Delivering Term versus Preterm Infants.					
31195757	7	31	from	milk	998:1001	arg1	different					977:985	different	977:985	different	977:985	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	7	31	from	milk	998:1001	arg1	concentrations					909:922	The concentrations	905:922	The concentrations of a number of sialylated oligosaccharides	905:965	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	8	32	from	age	1093:1095	arg1	concentrations					1102:1115	the concentrations	1098:1115	the concentrations of a number of HMOs	1098:1135	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	8	32	from	age	1093:1095	arg1	different					1156:1164	different	1156:1164	different	1156:1164	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	4	33	theme	preterm	584:590	arg1	infants					592:598	very preterm infants	579:598	very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight)	579:654	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	8	34	theme	postmenstrual	1079:1091	arg1	age					1093:1095	equivalent postmenstrual age	1068:1095	equivalent postmenstrual age	1068:1095	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	5	35	theme	most	721:724	arg1	HMOs					726:729	most HMOs	721:729	most HMOs	721:729	The concentrations of most HMOs were comparable at equivalent postpartum age.					
31195757	6	36	theme	first	879:883	arg1	month					885:889	the first month	875:889	the first month of lactation	875:902	However, HMOs containing α-1,2-linked fucose were reduced in concentration in preterm milk during the first month of lactation.					
31195757	1	37	from	infections	220:229	arg1	infant					208:213	the infant	204:213	the infant from infections	204:229	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	1	38	theme	Human	91:95	arg1	HMOs					120:123	HMOs	120:123	HMOs	120:123	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	1	38	theme	Human	91:95	arg1	oligosaccharides					102:117	Human milk oligosaccharides	91:117	Human milk oligosaccharides (HMOs)	91:124	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	1	38	theme	Human	91:95	arg1	component					138:146	a major component	130:146	a major component of human milk	130:160	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	4	39	from	mothers	557:563	arg1	samples					533:539	500 samples	529:539	500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants	529:696	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	4	39	from	mothers	557:563	arg1	milk					544:547	milk	544:547	milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants	544:696	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	5	40	theme	HMOs	726:729	arg1	comparable					736:745	comparable	736:745	comparable	736:745	The concentrations of most HMOs were comparable at equivalent postpartum age.					
31195757	5	40	theme	HMOs	726:729	arg1	concentrations					703:716	The concentrations	699:716	The concentrations of most HMOs	699:729	The concentrations of most HMOs were comparable at equivalent postpartum age.					
31195757	4	41	theme	breastfeeding	671:683	arg1	infants					690:696	breastfeeding term infants	671:696	breastfeeding term infants	671:696	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	1	42	theme	milk	97:100	arg1	HMOs					120:123	HMOs	120:123	HMOs	120:123	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	1	42	theme	milk	97:100	arg1	oligosaccharides					102:117	Human milk oligosaccharides	91:117	Human milk oligosaccharides (HMOs)	91:124	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	1	42	theme	milk	97:100	arg1	component					138:146	a major component	130:146	a major component of human milk	130:160	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	8	43	theme	equivalent	1068:1077	arg1	age					1093:1095	equivalent postmenstrual age	1068:1095	equivalent postmenstrual age	1068:1095	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	8	44	theme	term	1189:1192	arg1	milk					1194:1197	term milk	1189:1197	term milk	1189:1197	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	4	45	theme	gestational	615:625	arg1	age					627:629	gestational age	615:629	gestational age	615:629	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	10	46	theme	potential	1418:1426	arg1	impact					1437:1442	their potential clinical impact	1412:1442	their potential clinical impact	1412:1442	The observed differences warrant further investigation in view of their potential clinical impact.					
31195757	6	47	link	α-1,2-linked	802:813	arg1	fucose					815:820	α-1,2-linked fucose	802:820	α-1,2-linked fucose	802:820	However, HMOs containing α-1,2-linked fucose were reduced in concentration in preterm milk during the first month of lactation.					
31195757	9	48	theme	infants	1293:1299	arg1	milk					1280:1283	the milk	1276:1283	the milk of term infants	1276:1299	The largest differences manifest around 40 weeks of postmenstrual age, when the milk of term infants contains the highest concentrations of HMOs.					
31195757	4	49	theme	breastfeeding	565:577	arg1	mothers					557:563	25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants	554:696	mothers	557:563	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	3	50	theme	HMO	365:367	arg1	composition					369:379	the HMO composition	361:379	the HMO composition of preterm milk	361:395	This study aimed to determine if the HMO composition of preterm milk differed from that of term milk at equivalent stage of lactation and equivalent postmenstrual age.					
31195757	8	51	theme	HMOs	1132:1135	arg1	number					1122:1127	a number	1120:1127	a number of HMOs	1120:1135	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	6	52	contain	containing	791:800	arg1	HMOs					786:789	HMOs	786:789	HMOs containing α-1,2-linked fucose	786:820	However, HMOs containing α-1,2-linked fucose were reduced in concentration in preterm milk during the first month of lactation.					
31195757	6	52	contain	containing	791:800	arg2	fucose					815:820	α-1,2-linked fucose	802:820	α-1,2-linked fucose	802:820	However, HMOs containing α-1,2-linked fucose were reduced in concentration in preterm milk during the first month of lactation.					
31195757	4	53	dep	mothers	557:563	arg1	infants					592:598	very preterm infants	579:598	very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight)	579:654	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	4	53	dep	mothers	557:563	arg1	infants					690:696	breastfeeding term infants	671:696	breastfeeding term infants	671:696	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	1	54	theme	important	175:183	arg1	role					185:188	an important role	172:188	an important role	172:188	Human milk oligosaccharides (HMOs) are a major component of human milk, and play an important role in protecting the infant from infections.					
31195757	0	55	theme	Mothers	43:49	arg1	Milk					35:38	the Milk	31:38	the Milk of Mothers	31:49	Human Milk Oligosaccharides in the Milk of Mothers Delivering Term versus Preterm Infants.					
31195757	6	56	theme	preterm	855:861	arg1	milk					863:866	preterm milk	855:866	preterm milk	855:866	However, HMOs containing α-1,2-linked fucose were reduced in concentration in preterm milk during the first month of lactation.					
31195757	7	57	theme	oligosaccharides	950:965	arg1	number					929:934	a number	927:934	a number of sialylated oligosaccharides	927:965	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	7	58	gly	sialylated	939:948	arg1	oligosaccharides					950:965	sialylated oligosaccharides	939:965	sialylated oligosaccharides	939:965	The concentrations of a number of sialylated oligosaccharides were also different in preterm milk, in particular 3'-sialyllactose concentrations were elevated.					
31195757	4	59	theme	age	627:629	arg1	weeks					606:610	< 32 weeks	601:610	< 32 weeks of gestational age	601:629	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	4	59	theme	age	627:629	arg1	1500g					634:638	< 1500g	632:638	< 1500g of birthweight	632:653	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	9	60	theme	term	1288:1291	arg1	infants					1293:1299	term infants	1288:1299	term infants	1288:1299	The largest differences manifest around 40 weeks of postmenstrual age, when the milk of term infants contains the highest concentrations of HMOs.					
31195757	10	61	theme	impact	1437:1442	arg1	view					1404:1407	view	1404:1407	view of their potential clinical impact	1404:1442	The observed differences warrant further investigation in view of their potential clinical impact.					
31195757	8	62	from	preterm	1169:1175	arg1	concentrations					1102:1115	the concentrations	1098:1115	the concentrations of a number of HMOs	1098:1135	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	8	62	from	preterm	1169:1175	arg1	different					1156:1164	different	1156:1164	different	1156:1164	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	3	63	theme	equivalent	432:441	arg1	stage					443:447	equivalent stage	432:447	equivalent stage of lactation	432:460	This study aimed to determine if the HMO composition of preterm milk differed from that of term milk at equivalent stage of lactation and equivalent postmenstrual age.					
31195757	2	64	contain	have	281:284	arg1	infants					240:246	Preterm infants	232:246	Preterm infants	232:246	Preterm infants are particularly vulnerable, but have improved outcomes if fed with human milk.					
31195757	2	64	contain	have	281:284	arg2	outcomes					295:302	improved outcomes	286:302	improved outcomes	286:302	Preterm infants are particularly vulnerable, but have improved outcomes if fed with human milk.					
31195757	3	65	theme	term	419:422	arg1	milk					424:427	term milk	419:427	term milk	419:427	This study aimed to determine if the HMO composition of preterm milk differed from that of term milk at equivalent stage of lactation and equivalent postmenstrual age.					
31195757	10	66	theme	clinical	1428:1435	arg1	impact					1437:1442	their potential clinical impact	1412:1442	their potential clinical impact	1412:1442	The observed differences warrant further investigation in view of their potential clinical impact.					
31195757	3	67	theme	postmenstrual	477:489	arg1	age					491:493	equivalent postmenstrual age	466:493	equivalent postmenstrual age	466:493	This study aimed to determine if the HMO composition of preterm milk differed from that of term milk at equivalent stage of lactation and equivalent postmenstrual age.					
31195757	9	68	theme	HMOs	1340:1343	arg1	concentrations					1322:1335	the highest concentrations	1310:1335	the highest concentrations of HMOs	1310:1343	The largest differences manifest around 40 weeks of postmenstrual age, when the milk of term infants contains the highest concentrations of HMOs.					
31195757	4	69	theme	term	685:688	arg1	infants					690:696	breastfeeding term infants	671:696	breastfeeding term infants	671:696	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	2	70	theme	improved	286:293	arg1	outcomes					295:302	improved outcomes	286:302	improved outcomes	286:302	Preterm infants are particularly vulnerable, but have improved outcomes if fed with human milk.					
31195757	4	71	theme	<	632:632	arg1	weeks					606:610	< 32 weeks	601:610	< 32 weeks of gestational age	601:629	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	4	71	theme	<	632:632	arg1	1500g					634:638	< 1500g	632:638	< 1500g of birthweight	632:653	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	0	72	theme	Term	62:65	arg1	Infants					82:88	Term versus Preterm Infants	62:88	Infants	82:88	Human Milk Oligosaccharides in the Milk of Mothers Delivering Term versus Preterm Infants.					
31195757	5	73	theme	equivalent	750:759	arg1	age					772:774	equivalent postpartum age	750:774	equivalent postpartum age	750:774	The concentrations of most HMOs were comparable at equivalent postpartum age.					
31195757	3	74	theme	lactation	452:460	arg1	age					491:493	equivalent postmenstrual age	466:493	equivalent postmenstrual age	466:493	This study aimed to determine if the HMO composition of preterm milk differed from that of term milk at equivalent stage of lactation and equivalent postmenstrual age.					
31195757	3	74	theme	lactation	452:460	arg1	stage					443:447	equivalent stage	432:447	equivalent stage of lactation	432:460	This study aimed to determine if the HMO composition of preterm milk differed from that of term milk at equivalent stage of lactation and equivalent postmenstrual age.					
31195757	3	75	theme	milk	392:395	arg1	composition					369:379	the HMO composition	361:379	the HMO composition of preterm milk	361:395	This study aimed to determine if the HMO composition of preterm milk differed from that of term milk at equivalent stage of lactation and equivalent postmenstrual age.					
31195757	4	76	theme	milk	544:547	arg1	samples					533:539	500 samples	529:539	500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants	529:696	In all, 22 HMOs were analyzed in 500 samples of milk from 25 mothers breastfeeding very preterm infants (< 32 weeks of gestational age, < 1500g of birthweight) and 28 mothers breastfeeding term infants.					
31195757	9	77	theme	largest	1204:1210	arg1	differences					1212:1222	The largest differences	1200:1222	The largest differences	1200:1222	The largest differences manifest around 40 weeks of postmenstrual age, when the milk of term infants contains the highest concentrations of HMOs.					
31195757	8	78	theme	number	1122:1127	arg1	different					1156:1164	different	1156:1164	different	1156:1164	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	8	78	theme	number	1122:1127	arg1	concentrations					1102:1115	the concentrations	1098:1115	the concentrations of a number of HMOs	1098:1135	At equivalent postmenstrual age, the concentrations of a number of HMOs were significantly different in preterm compared to term milk.					
31195757	9	79	theme	postmenstrual	1252:1264	arg1	age					1266:1268	postmenstrual age	1252:1268	postmenstrual age	1252:1268	The largest differences manifest around 40 weeks of postmenstrual age, when the milk of term infants contains the highest concentrations of HMOs.					
31284012	0	0	theme	aminopropyltriethoxysilane-modified	60:94	arg1	beads					165:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	6	1	theme	kinetic	1017:1023	arg1	models					1058:1063	the pseudo-second-order kinetic and Langmuir adsorption isotherm models	993:1063	the pseudo-second-order kinetic and Langmuir adsorption isotherm models	993:1063	The adsorption process conforms to the pseudo-second-order kinetic and Langmuir adsorption isotherm models.					
31284012	2	2	theme	X-ray	588:592	arg1	diffraction					594:604	X-ray diffraction	588:604	X-ray diffraction	588:604	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	2	3	theme	infrared	531:538	arg1	spectroscopy					540:551	Fourier-transform infrared spectroscopy	513:551	Fourier-transform infrared spectroscopy	513:551	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	2	4	theme	electron	563:570	arg1	microscopy					572:581	scanning electron microscopy	554:581	scanning electron microscopy	554:581	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	7	5	theme	Pb	1084:1085	arg1	adsorption					1070:1079	The adsorption	1066:1079	The adsorption of Pb(II)	1066:1089	The adsorption of Pb(II) reaches a maximum of 625.34 mg/g, which compares favorably with other reported adsorbents.					
31284012	7	6	theme	625.34 mg/g	1112:1122	arg1	maximum					1101:1107	a maximum	1099:1107	a maximum	1099:1107	The adsorption of Pb(II) reaches a maximum of 625.34 mg/g, which compares favorably with other reported adsorbents.					
31284012	8	7	theme	@	1218:1218	arg1	hybrid					1237:1242	a promising hybrid	1225:1242	a promising hybrid adsorbent for effective removal of Pb(II) from water	1225:1295	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	8	7	theme	@	1218:1218	arg1	CS					1219:1220	APTS-Fe3O4/APT@CS	1204:1220	APTS-Fe3O4/APT@CS	1204:1220	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	5	8	theme	Fe3O4/APT	897:905	arg1	capacity					885:892	The adsorption capacity	870:892	The adsorption capacity of Fe3O4/APT	870:905	The adsorption capacity of Fe3O4/APT was improved by the modification with APTS and CS.					
31284012	0	9	theme	attapulgite	105:115	arg1	CS					142:143	APTS-Fe3O4/APT@CS	127:143	APTS-Fe3O4/APT@CS	127:143	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	0	9	theme	attapulgite	105:115	arg1	chitosan					117:124	attapulgite@chitosan	105:124	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	3	10	theme	vibrating	670:678	arg1	magnetometer					687:698	a vibrating sample magnetometer	668:698	a vibrating sample magnetometer	668:698	The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight analysis.					
31284012	0	11	theme	magnetic	96:103	arg1	beads					165:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	0	12	theme	chitosan	117:124	arg1	beads					165:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	3	13	theme	heat	713:716	arg1	resistance					718:727	their heat resistance	707:727	their heat resistance	707:727	The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight analysis.					
31284012	4	14	theme	capacities	836:845	arg1	comparison					811:820	comparison	811:820	comparison of adsorption capacities	811:845	A recycling test and comparison of adsorption capacities were also carried out.					
31284012	4	14	theme	capacities	836:845	arg1	test					802:805	A recycling test	790:805	A recycling test	790:805	A recycling test and comparison of adsorption capacities were also carried out.					
31284012	0	15	theme	@	116:116	arg1	CS					142:143	APTS-Fe3O4/APT@CS	127:143	APTS-Fe3O4/APT@CS	127:143	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	0	15	theme	@	116:116	arg1	chitosan					117:124	attapulgite@chitosan	105:124	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	4	16	theme	adsorption	825:834	arg1	capacities					836:845	adsorption capacities	825:845	adsorption capacities	825:845	A recycling test and comparison of adsorption capacities were also carried out.					
31284012	2	17	theme	crystal	481:487	arg1	phases					489:494	crystal phases	481:494	crystal phases	481:494	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	7	18	theme	reported	1161:1168	arg1	adsorbents					1170:1179	other reported adsorbents	1155:1179	other reported adsorbents	1155:1179	The adsorption of Pb(II) reaches a maximum of 625.34 mg/g, which compares favorably with other reported adsorbents.					
31284012	6	19	theme	adsorption	962:971	arg1	process					973:979	The adsorption process	958:979	The adsorption process	958:979	The adsorption process conforms to the pseudo-second-order kinetic and Langmuir adsorption isotherm models.					
31284012	2	20	theme	scanning	554:561	arg1	microscopy					572:581	scanning electron microscopy	554:581	scanning electron microscopy	554:581	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	0	21	theme	Efficient	0:8	arg1	adsorption					10:19	Efficient adsorption	0:19	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.	0:170	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	1	22	theme	aqueous	255:261	arg1	solutions					263:271	aqueous solutions	255:271	aqueous solutions	255:271	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions could be improved by modifying the particles with aminopropyltriethoxysilane (APTS) and then combining with chitosan (CS) into beads.					
31284012	1	23	from	solutions	263:271	arg1	performance					176:186	The performance	172:186	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions	172:271	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions could be improved by modifying the particles with aminopropyltriethoxysilane (APTS) and then combining with chitosan (CS) into beads.					
31284012	8	24	theme	promising	1227:1235	arg1	hybrid					1237:1242	a promising hybrid	1225:1242	a promising hybrid adsorbent for effective removal of Pb(II) from water	1225:1295	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	8	24	theme	promising	1227:1235	arg1	CS					1219:1220	APTS-Fe3O4/APT@CS	1204:1220	APTS-Fe3O4/APT@CS	1204:1220	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	0	25	theme	Pb	24:25	arg1	adsorption					10:19	Efficient adsorption	0:19	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.	0:170	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	0	26	theme	APTS-Fe3O4/APT	127:140	arg1	CS					142:143	APTS-Fe3O4/APT@CS	127:143	APTS-Fe3O4/APT@CS	127:143	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	0	26	theme	APTS-Fe3O4/APT	127:140	arg1	chitosan					117:124	attapulgite@chitosan	105:124	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	1	27	theme	Fe3O4/attapulgite	191:207	arg1	nanoparticles					215:227	Fe3O4/attapulgite (APT) nanoparticles	191:227	Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption	191:248	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions could be improved by modifying the particles with aminopropyltriethoxysilane (APTS) and then combining with chitosan (CS) into beads.					
31284012	7	28	theme	other	1155:1159	arg1	adsorbents					1170:1179	other reported adsorbents	1155:1179	other reported adsorbents	1155:1179	The adsorption of Pb(II) reaches a maximum of 625.34 mg/g, which compares favorably with other reported adsorbents.					
31284012	2	29	theme	Fourier-transform	513:529	arg1	spectroscopy					540:551	Fourier-transform infrared spectroscopy	513:551	Fourier-transform infrared spectroscopy	513:551	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	3	30	theme	APTS-Fe3O4/APT	634:647	arg1	CS					649:650	APTS-Fe3O4/APT@CS	634:650	APTS-Fe3O4/APT@CS	634:650	The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight analysis.					
31284012	8	31	theme	adsorbent	1244:1252	arg1	hybrid					1237:1242	a promising hybrid	1225:1242	a promising hybrid adsorbent for effective removal of Pb(II) from water	1225:1295	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	8	31	theme	adsorbent	1244:1252	arg1	CS					1219:1220	APTS-Fe3O4/APT@CS	1204:1220	APTS-Fe3O4/APT@CS	1204:1220	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	8	32	theme	APTS-Fe3O4/APT	1204:1217	arg1	hybrid					1237:1242	a promising hybrid	1225:1242	a promising hybrid adsorbent for effective removal of Pb(II) from water	1225:1295	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	8	32	theme	APTS-Fe3O4/APT	1204:1217	arg1	CS					1219:1220	APTS-Fe3O4/APT@CS	1204:1220	APTS-Fe3O4/APT@CS	1204:1220	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	3	33	theme	@	648:648	arg1	CS					649:650	APTS-Fe3O4/APT@CS	634:650	APTS-Fe3O4/APT@CS	634:650	The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight analysis.					
31284012	6	34	theme	isotherm	1049:1056	arg1	models					1058:1063	the pseudo-second-order kinetic and Langmuir adsorption isotherm models	993:1063	the pseudo-second-order kinetic and Langmuir adsorption isotherm models	993:1063	The adsorption process conforms to the pseudo-second-order kinetic and Langmuir adsorption isotherm models.					
31284012	2	35	theme	CS	442:443	arg1	beads					445:449	the APTS-Fe3O4/APT@CS beads	423:449	the APTS-Fe3O4/APT@CS beads	423:449	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	3	36	theme	thermal	765:771	arg1	analysis					780:787	thermal weight analysis	765:787	thermal weight analysis	765:787	The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight analysis.					
31284012	0	37	theme	@	141:141	arg1	CS					142:143	APTS-Fe3O4/APT@CS	127:143	APTS-Fe3O4/APT@CS	127:143	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	0	37	theme	@	141:141	arg1	chitosan					117:124	attapulgite@chitosan	105:124	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	0	38	from	solutions	44:52	arg1	adsorption					10:19	Efficient adsorption	0:19	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.	0:170	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	5	39	with	modification	927:938	arg1	APTS					945:948	APTS	945:948	APTS	945:948	The adsorption capacity of Fe3O4/APT was improved by the modification with APTS and CS.					
31284012	5	39	with	modification	927:938	arg1	CS					954:955	CS	954:955	CS	954:955	The adsorption capacity of Fe3O4/APT was improved by the modification with APTS and CS.					
31284012	6	40	theme	adsorption	1038:1047	arg1	models					1058:1063	the pseudo-second-order kinetic and Langmuir adsorption isotherm models	993:1063	the pseudo-second-order kinetic and Langmuir adsorption isotherm models	993:1063	The adsorption process conforms to the pseudo-second-order kinetic and Langmuir adsorption isotherm models.					
31284012	2	41	theme	@	441:441	arg1	beads					445:449	the APTS-Fe3O4/APT@CS beads	423:449	the APTS-Fe3O4/APT@CS beads	423:449	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	3	42	theme	weight	773:778	arg1	analysis					780:787	thermal weight analysis	765:787	thermal weight analysis	765:787	The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight analysis.					
31284012	0	43	theme	composite	146:154	arg1	beads					165:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	2	44	theme	surface	458:464	arg1	morphology					466:475	surface morphology	458:475	surface morphology	458:475	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	8	45	theme	Pb	1279:1280	arg1	removal					1268:1274	effective removal	1258:1274	effective removal of Pb(II) from water	1258:1295	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	2	46	theme	APTS-Fe3O4/APT	427:440	arg1	beads					445:449	the APTS-Fe3O4/APT@CS beads	423:449	the APTS-Fe3O4/APT@CS beads	423:449	After preparing the APTS-Fe3O4/APT@CS beads, their surface morphology and crystal phases were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction.					
31284012	1	47	from	adsorption	239:248	arg1	performance					176:186	The performance	172:186	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions	172:271	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions could be improved by modifying the particles with aminopropyltriethoxysilane (APTS) and then combining with chitosan (CS) into beads.					
31284012	8	48	from	water	1291:1295	arg1	removal					1268:1274	effective removal	1258:1274	effective removal of Pb(II) from water	1258:1295	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	1	49	from	nanoparticles	215:227	arg1	adsorption					239:248	Pb(II) adsorption	232:248	Pb(II) adsorption	232:248	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions could be improved by modifying the particles with aminopropyltriethoxysilane (APTS) and then combining with chitosan (CS) into beads.					
31284012	1	50	theme	nanoparticles	215:227	arg1	performance					176:186	The performance	172:186	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions	172:271	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions could be improved by modifying the particles with aminopropyltriethoxysilane (APTS) and then combining with chitosan (CS) into beads.					
31284012	0	51	theme	hydrogel	156:163	arg1	beads					165:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	60:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	3	52	theme	CS	649:650	arg1	properties					620:629	The magnetic properties	607:629	The magnetic properties of APTS-Fe3O4/APT@CS	607:650	The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight analysis.					
31284012	3	53	theme	magnetic	611:618	arg1	properties					620:629	The magnetic properties	607:629	The magnetic properties of APTS-Fe3O4/APT@CS	607:650	The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight analysis.					
31284012	1	54	from	performance	176:186	arg1	adsorption					239:248	Pb(II) adsorption	232:248	Pb(II) adsorption	232:248	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions could be improved by modifying the particles with aminopropyltriethoxysilane (APTS) and then combining with chitosan (CS) into beads.					
31284012	8	55	theme	effective	1258:1266	arg1	removal					1268:1274	effective removal	1258:1274	effective removal of Pb(II) from water	1258:1295	The results show that APTS-Fe3O4/APT@CS is a promising hybrid adsorbent for effective removal of Pb(II) from water.					
31284012	4	56	theme	recycling	792:800	arg1	test					802:805	A recycling test	790:805	A recycling test	790:805	A recycling test and comparison of adsorption capacities were also carried out.					
31284012	5	57	theme	adsorption	874:883	arg1	capacity					885:892	The adsorption capacity	870:892	The adsorption capacity of Fe3O4/APT	870:905	The adsorption capacity of Fe3O4/APT was improved by the modification with APTS and CS.					
31284012	1	58	theme	Pb	232:233	arg1	adsorption					239:248	Pb(II) adsorption	232:248	Pb(II) adsorption	232:248	The performance of Fe3O4/attapulgite (APT) nanoparticles in Pb(II) adsorption from aqueous solutions could be improved by modifying the particles with aminopropyltriethoxysilane (APTS) and then combining with chitosan (CS) into beads.					
31284012	0	59	theme	aqueous	36:42	arg1	solutions					44:52	aqueous solutions	36:52	aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads	36:169	Efficient adsorption of Pb(II) from aqueous solutions using aminopropyltriethoxysilane-modified magnetic attapulgite@chitosan (APTS-Fe3O4/APT@CS) composite hydrogel beads.					
31284012	3	60	theme	sample	680:685	arg1	magnetometer					687:698	a vibrating sample magnetometer	668:698	a vibrating sample magnetometer	668:698	The magnetic properties of APTS-Fe3O4/APT@CS were studied by a vibrating sample magnetometer, while their heat resistance and stability were characterized by thermal weight analysis.					
31284012	6	61	theme	Langmuir	1029:1036	arg1	adsorption					1038:1047	Langmuir adsorption	1029:1047	Langmuir adsorption	1029:1047	The adsorption process conforms to the pseudo-second-order kinetic and Langmuir adsorption isotherm models.					
31284012	6	62	theme	pseudo-second-order	997:1015	arg1	models					1058:1063	the pseudo-second-order kinetic and Langmuir adsorption isotherm models	993:1063	the pseudo-second-order kinetic and Langmuir adsorption isotherm models	993:1063	The adsorption process conforms to the pseudo-second-order kinetic and Langmuir adsorption isotherm models.					
31255278	0	0	theme	milk	70:73	arg1	production					75:84	milk production	70:84	milk production	70:84	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	6	1	theme	mixed	837:841	arg1	ration					843:848	a total mixed ration	829:848	a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch	829:959	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	0	2	with	starch	27:32	arg1	glycerol					39:46	glycerol	39:46	glycerol	39:46	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	3	3	theme	dairy	451:455	arg1	cows					457:460	dairy cows	451:460	dairy cows with energy for milk production	451:492	Glycerol could thus supply dairy cows with energy for milk production without contributing much to methane production.					
31255278	0	4	theme	grass	125:129	arg1	diets					144:148	grass silage-based diets	125:148	grass silage-based diets	125:148	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	7	5	theme	higher	1033:1038	arg1	kg/d					1073:1076	21.6 vs. 20.1 kg/d	1059:1076	21.6 vs. 20.1 kg/d	1059:1076	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	5	theme	higher	1033:1038	arg1	intake					1051:1056	higher dry matter intake	1033:1056	higher dry matter intake (21.6 vs. 20.1 kg/d)	1033:1077	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	1	6	theme	meat	297:300	arg1	kilogram					277:284	kilogram	277:284	kilogram of milk or meat	277:300	To lower the effect of climate change from cattle production, we should aim at decreasing their enteric methane emissions per kilogram of milk or meat.					
31255278	3	7	with	cows	457:460	arg1	energy					467:472	energy	467:472	energy	467:472	Glycerol could thus supply dairy cows with energy for milk production without contributing much to methane production.					
31255278	9	8	theme	dairy	1487:1491	arg1	cows					1493:1496	dairy cows	1487:1496	dairy cows	1487:1496	Compared with wheat starch, adding refined glycerol (200 g/kg of dry matter) to the feed of dairy cows does not seem to have the potential to decrease enteric methane emissions.					
31255278	0	9	from	Effects	0:6	arg1	efficiency					96:105	feed efficiency	91:105	feed efficiency	91:105	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	0	9	from	Effects	0:6	arg1	emissions					59:67	methane emissions	51:67	methane emissions	51:67	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	0	9	from	Effects	0:6	arg1	production					75:84	milk production	70:84	milk production	70:84	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	6	10	theme	ad	991:992	arg1	libitum					994:1000	glycerol (200) fed ad libitum	972:1000	glycerol (200) fed ad libitum	972:1000	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	7	11	theme	milk	1219:1222	arg1	kg/d					1245:1248	28.4 vs. 29.7 kg/d	1231:1248	28.4 vs. 29.7 kg/d	1231:1248	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	11	theme	milk	1219:1222	arg1	yield					1224:1228	energy-corrected milk yield	1202:1228	energy-corrected milk yield (28.4 vs. 29.7 kg/d)	1202:1249	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	5	12	theme	Swedish	682:688	arg1	cows					694:697	Twenty-two Swedish Red cows	671:697	Twenty-two Swedish Red cows in mid lactation	671:714	Twenty-two Swedish Red cows in mid lactation were used in a switch-back, change-over experiment with 3 periods of 21 d.					
31255278	9	13	theme	wheat	1409:1413	arg1	starch					1415:1420	wheat starch	1409:1420	wheat starch	1409:1420	Compared with wheat starch, adding refined glycerol (200 g/kg of dry matter) to the feed of dairy cows does not seem to have the potential to decrease enteric methane emissions.					
31255278	7	14	theme	energy-corrected	1202:1217	arg1	kg/d					1245:1248	28.4 vs. 29.7 kg/d	1231:1248	28.4 vs. 29.7 kg/d	1231:1248	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	14	theme	energy-corrected	1202:1217	arg1	yield					1224:1228	energy-corrected milk yield	1202:1228	energy-corrected milk yield (28.4 vs. 29.7 kg/d)	1202:1249	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	6	15	theme	grass	880:884	arg1	605					894:896	605	894:896	605	894:896	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	6	15	theme	grass	880:884	arg1	silage					886:891	grass silage	880:891	grass silage (605)	880:897	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	0	16	theme	feed	91:94	arg1	efficiency					96:105	feed efficiency	91:105	feed efficiency	91:105	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	6	17	theme	glycerol	972:979	arg1	libitum					994:1000	glycerol (200) fed ad libitum	972:1000	glycerol (200) fed ad libitum	972:1000	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	5	18	theme	d	788:788	arg1	periods					774:780	3 periods	772:780	3 periods of 21 d	772:788	Twenty-two Swedish Red cows in mid lactation were used in a switch-back, change-over experiment with 3 periods of 21 d.					
31255278	5	19	theme	switch-back	731:741	arg1	experiment					756:765	a switch-back, change-over experiment	729:765	a switch-back, change-over experiment with 3 periods of 21 d	729:788	Twenty-two Swedish Red cows in mid lactation were used in a switch-back, change-over experiment with 3 periods of 21 d.					
31255278	8	20	dep	extent	1364:1369	arg1	assumed					1386:1392	assumed	1386:1392	assumed	1386:1392	These results indicate that when glycerol is mixed with the feed, it is available to rumen microbes to a larger extent than initially assumed.					
31255278	5	21	dep	switch-back	731:741	arg1	change-over					744:754	change-over	744:754	change-over	744:754	Twenty-two Swedish Red cows in mid lactation were used in a switch-back, change-over experiment with 3 periods of 21 d.					
31255278	3	22	theme	milk	478:481	arg1	production					483:492	milk production	478:492	milk production	478:492	Glycerol could thus supply dairy cows with energy for milk production without contributing much to methane production.					
31255278	2	23	theme	rumen	340:344	arg1	epithelium					346:355	the rumen epithelium	336:355	the rumen epithelium	336:355	Glycerol may be absorbed through the rumen epithelium and would consequently be less available to microbes in the rumen.					
31255278	6	24	theme	fed	987:989	arg1	libitum					994:1000	glycerol (200) fed ad libitum	972:1000	glycerol (200) fed ad libitum	972:1000	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	7	25	theme	dry	1040:1042	arg1	kg/d					1073:1076	21.6 vs. 20.1 kg/d	1059:1076	21.6 vs. 20.1 kg/d	1059:1076	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	25	theme	dry	1040:1042	arg1	intake					1051:1056	higher dry matter intake	1033:1056	higher dry matter intake (21.6 vs. 20.1 kg/d)	1033:1077	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	5	26	with	experiment	756:765	arg1	periods					774:780	3 periods	772:780	3 periods of 21 d	772:788	Twenty-two Swedish Red cows in mid lactation were used in a switch-back, change-over experiment with 3 periods of 21 d.					
31255278	9	27	theme	dry	1460:1462	arg1	matter					1464:1469	dry matter	1460:1469	dry matter	1460:1469	Compared with wheat starch, adding refined glycerol (200 g/kg of dry matter) to the feed of dairy cows does not seem to have the potential to decrease enteric methane emissions.					
31255278	6	28	theme	dry	868:870	arg1	matter					872:877	dry matter	868:877	dry matter	868:877	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	2	29	from	microbes	401:408	arg1	rumen					417:421	the rumen	413:421	the rumen	413:421	Glycerol may be absorbed through the rumen epithelium and would consequently be less available to microbes in the rumen.					
31255278	7	30	located	found	1193:1197	arg1	kg/d					1245:1248	28.4 vs. 29.7 kg/d	1231:1248	28.4 vs. 29.7 kg/d	1231:1248	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	30	located	found	1193:1197	arg2	difference					1178:1187	no difference	1175:1187	no difference	1175:1187	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	30	located	found	1193:1197	arg1	yield					1224:1228	energy-corrected milk yield	1202:1228	energy-corrected milk yield (28.4 vs. 29.7 kg/d)	1202:1249	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	9	31	theme	refined	1430:1436	arg1	glycerol					1438:1445	refined glycerol	1430:1445	refined glycerol (200 g/kg of dry matter)	1430:1470	Compared with wheat starch, adding refined glycerol (200 g/kg of dry matter) to the feed of dairy cows does not seem to have the potential to decrease enteric methane emissions.					
31255278	3	32	theme	methane	523:529	arg1	production					531:540	methane production	523:540	methane production	523:540	Glycerol could thus supply dairy cows with energy for milk production without contributing much to methane production.					
31255278	4	33	theme	milk	618:621	arg1	production					623:632	milk production	618:632	milk production	618:632	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	0	34	theme	wheat	21:25	arg1	starch					27:32	replacing wheat starch	11:32	replacing wheat starch with glycerol	11:46	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	6	35	theme	matter	872:877	arg1	g/kg					860:863	g/kg	860:863	g/kg of dry matter	860:877	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	9	36	theme	cows	1493:1496	arg1	feed					1479:1482	the feed	1475:1482	the feed of dairy cows	1475:1496	Compared with wheat starch, adding refined glycerol (200 g/kg of dry matter) to the feed of dairy cows does not seem to have the potential to decrease enteric methane emissions.					
31255278	9	37	theme	enteric	1546:1552	arg1	emissions					1562:1570	enteric methane emissions	1546:1570	enteric methane emissions	1546:1570	Compared with wheat starch, adding refined glycerol (200 g/kg of dry matter) to the feed of dairy cows does not seem to have the potential to decrease enteric methane emissions.					
31255278	4	38	theme	wheat	588:592	arg1	starch					594:599	replacing wheat starch	578:599	replacing wheat starch with glycerol	578:613	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	0	39	theme	replacing	11:19	arg1	starch					27:32	replacing wheat starch	11:32	replacing wheat starch with glycerol	11:46	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	1	40	theme	enteric	247:253	arg1	emissions					263:271	their enteric methane emissions	241:271	their enteric methane emissions	241:271	To lower the effect of climate change from cattle production, we should aim at decreasing their enteric methane emissions per kilogram of milk or meat.					
31255278	9	41	dep	glycerol	1438:1445	arg1	g/kg					1452:1455	200 g/kg	1448:1455	200 g/kg of dry matter	1448:1469	Compared with wheat starch, adding refined glycerol (200 g/kg of dry matter) to the feed of dairy cows does not seem to have the potential to decrease enteric methane emissions.					
31255278	9	42	theme	matter	1464:1469	arg1	g/kg					1452:1455	200 g/kg	1448:1455	200 g/kg of dry matter	1448:1469	Compared with wheat starch, adding refined glycerol (200 g/kg of dry matter) to the feed of dairy cows does not seem to have the potential to decrease enteric methane emissions.					
31255278	4	43	theme	replacing	578:586	arg1	starch					594:599	replacing wheat starch	578:599	replacing wheat starch with glycerol	578:613	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	1	44	theme	methane	255:261	arg1	emissions					263:271	their enteric methane emissions	241:271	their enteric methane emissions	241:271	To lower the effect of climate change from cattle production, we should aim at decreasing their enteric methane emissions per kilogram of milk or meat.					
31255278	7	45	theme	glycerol	1007:1014	arg1	diet					1016:1019	The glycerol diet	1003:1019	The glycerol diet	1003:1019	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	5	46	from	cows	694:697	arg1	lactation					706:714	mid lactation	702:714	mid lactation	702:714	Twenty-two Swedish Red cows in mid lactation were used in a switch-back, change-over experiment with 3 periods of 21 d.					
31255278	4	47	theme	starch	594:599	arg1	effect					568:573	the effect	564:573	the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions	564:668	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	1	48	theme	climate	174:180	arg1	change					182:187	climate change	174:187	climate change	174:187	To lower the effect of climate change from cattle production, we should aim at decreasing their enteric methane emissions per kilogram of milk or meat.					
31255278	0	49	theme	starch	27:32	arg1	Effects					0:6	Effects	0:6	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows	0:119	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	0	50	theme	dairy	110:114	arg1	cows					116:119	dairy cows	110:119	dairy cows	110:119	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	0	51	from	emissions	59:67	arg1	cows					116:119	dairy cows	110:119	dairy cows	110:119	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	8	52	theme	larger	1357:1362	arg1	extent					1364:1369	a larger extent	1355:1369	a larger extent than initially assumed	1355:1392	These results indicate that when glycerol is mixed with the feed, it is available to rumen microbes to a larger extent than initially assumed.					
31255278	1	53	theme	change	182:187	arg1	effect					164:169	the effect	160:169	the effect of climate change from cattle production	160:210	To lower the effect of climate change from cattle production, we should aim at decreasing their enteric methane emissions per kilogram of milk or meat.					
31255278	1	54	from	production	201:210	arg1	effect					164:169	the effect	160:169	the effect of climate change from cattle production	160:210	To lower the effect of climate change from cattle production, we should aim at decreasing their enteric methane emissions per kilogram of milk or meat.					
31255278	4	55	from	effect	568:573	arg1	intake					640:645	feed intake	635:645	feed intake	635:645	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	4	55	from	effect	568:573	arg1	emissions					660:668	methane emissions	652:668	methane emissions	652:668	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	4	55	from	effect	568:573	arg1	production					623:632	milk production	618:632	milk production	618:632	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	7	56	theme	wheat	1153:1157	arg1	starch					1159:1164	wheat starch	1153:1164	wheat starch	1153:1164	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	6	57	theme	wheat	948:952	arg1	starch					954:959	either wheat starch	941:959	either wheat starch	941:959	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	4	58	theme	methane	652:658	arg1	emissions					660:668	methane emissions	652:668	methane emissions	652:668	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	5	59	used	used	721:724	arg2	cows					694:697	Twenty-two Swedish Red cows	671:697	Twenty-two Swedish Red cows in mid lactation	671:714	Twenty-two Swedish Red cows in mid lactation were used in a switch-back, change-over experiment with 3 periods of 21 d.					
31255278	8	60	theme	rumen	1337:1341	arg1	microbes					1343:1350	rumen microbes	1337:1350	rumen microbes	1337:1350	These results indicate that when glycerol is mixed with the feed, it is available to rumen microbes to a larger extent than initially assumed.					
31255278	6	61	dep	silage	886:891	arg1	g/kg					860:863	g/kg	860:863	g/kg of dry matter	860:877	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	6	62	theme	total	831:835	arg1	ration					843:848	a total mixed ration	829:848	a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch	829:959	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	6	63	theme	dietary	797:803	arg1	treatments					805:814	The 2 dietary treatments	791:814	The 2 dietary treatments	791:814	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	9	64	theme	methane	1554:1560	arg1	emissions					1562:1570	enteric methane emissions	1546:1570	enteric methane emissions	1546:1570	Compared with wheat starch, adding refined glycerol (200 g/kg of dry matter) to the feed of dairy cows does not seem to have the potential to decrease enteric methane emissions.					
31255278	6	65	theme	rapeseed	900:907	arg1	120					915:917	120	915:917	120	915:917	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	6	65	theme	rapeseed	900:907	arg1	meal					909:912	rapeseed meal	900:912	rapeseed meal (120)	900:918	The 2 dietary treatments consisted of a total mixed ration based on (g/kg of dry matter) grass silage (605), rapeseed meal (120), and barley (70) and either wheat starch or refined glycerol (200) fed ad libitum.					
31255278	7	66	dep	resulted	1021:1028	arg1	compared					1119:1126	compared	1119:1126	compared with the diet containing wheat starch	1119:1164	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	66	dep	resulted	1021:1028	arg1	whereas					1167:1173	whereas	1167:1173	whereas	1167:1173	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	4	67	with	starch	594:599	arg1	glycerol					606:613	glycerol	606:613	glycerol	606:613	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	5	68	theme	mid	702:704	arg1	lactation					706:714	mid lactation	702:714	mid lactation	702:714	Twenty-two Swedish Red cows in mid lactation were used in a switch-back, change-over experiment with 3 periods of 21 d.					
31255278	5	69	theme	Red	690:692	arg1	cows					694:697	Twenty-two Swedish Red cows	671:697	Twenty-two Swedish Red cows in mid lactation	671:714	Twenty-two Swedish Red cows in mid lactation were used in a switch-back, change-over experiment with 3 periods of 21 d.					
31255278	0	70	theme	silage-based	131:142	arg1	diets					144:148	grass silage-based diets	125:148	grass silage-based diets	125:148	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	1	71	theme	cattle	194:199	arg1	production					201:210	cattle production	194:210	cattle production	194:210	To lower the effect of climate change from cattle production, we should aim at decreasing their enteric methane emissions per kilogram of milk or meat.					
31255278	7	72	theme	matter	1044:1049	arg1	kg/d					1073:1076	21.6 vs. 20.1 kg/d	1059:1076	21.6 vs. 20.1 kg/d	1059:1076	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	72	theme	matter	1044:1049	arg1	intake					1051:1056	higher dry matter intake	1033:1056	higher dry matter intake (21.6 vs. 20.1 kg/d)	1033:1077	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	0	73	from	production	75:84	arg1	cows					116:119	dairy cows	110:119	dairy cows	110:119	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	4	74	theme	feed	635:638	arg1	intake					640:645	feed intake	635:645	feed intake	635:645	This study evaluated the effect of replacing wheat starch with glycerol on milk production, feed intake, and methane emissions.					
31255278	7	75	contain	containing	1142:1151	arg1	diet					1137:1140	the diet	1133:1140	the diet containing wheat starch	1133:1164	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	75	contain	containing	1142:1151	arg2	starch					1159:1164	wheat starch	1153:1164	wheat starch	1153:1164	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	0	76	theme	methane	51:57	arg1	emissions					59:67	methane emissions	51:67	methane emissions	51:67	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31255278	1	77	theme	milk	289:292	arg1	kilogram					277:284	kilogram	277:284	kilogram of milk or meat	277:300	To lower the effect of climate change from cattle production, we should aim at decreasing their enteric methane emissions per kilogram of milk or meat.					
31255278	7	78	theme	methane	1083:1089	arg1	g/d					1114:1116	482 vs. 423 g/d	1102:1116	482 vs. 423 g/d	1102:1116	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	7	78	theme	methane	1083:1089	arg1	emissions					1091:1099	methane emissions	1083:1099	methane emissions (482 vs. 423 g/d)	1083:1117	The glycerol diet resulted in higher dry matter intake (21.6 vs. 20.1 kg/d) and methane emissions (482 vs. 423 g/d) compared with the diet containing wheat starch, whereas no difference was found in energy-corrected milk yield (28.4 vs. 29.7 kg/d).					
31255278	0	79	from	efficiency	96:105	arg1	cows					116:119	dairy cows	110:119	dairy cows	110:119	Effects of replacing wheat starch with glycerol on methane emissions, milk production, and feed efficiency in dairy cows fed grass silage-based diets.					
31722762	0	0	theme	C17	64:66	arg1	acids					89:93	C17 branched-chain fatty acids	64:93	C17 branched-chain fatty acids	64:93	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	3	1	theme	d	673:673	arg1	periods					659:665	two experimental periods	642:665	two experimental periods of 25 d	642:673	Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d.					
31722762	3	1	theme	d	673:673	arg1	animals					616:622	four animals	611:622	four animals per treatment	611:636	Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d.					
31722762	6	2	theme	different	1140:1148	arg1	columns					1160:1166	two different capillary columns	1136:1166	two different capillary columns	1136:1166	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	2	3	theme	regression	315:324	arg1	model					326:330	a linear regression model	306:330	this a linear regression model	301:330	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	7	4	theme	iso	1287:1289	arg1	15:0					1291:1294	iso 15:0	1287:1294	iso 15:0	1287:1294	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	1	5	theme	milk	214:217	arg1	fat					219:221	milk fat	214:221	milk fat	214:221	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	2	6	theme	anteiso	488:494	arg1	15:0					496:499	anteiso 15:0	488:499	anteiso 15:0	488:499	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	2	7	theme	linear	308:313	arg1	model					326:330	a linear regression model	306:330	this a linear regression model	301:330	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	6	8	from	acids	1073:1077	arg1	fat					1087:1089	milk fat	1082:1089	milk fat	1082:1089	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	0	9	theme	fatty	83:87	arg1	acids					89:93	C17 branched-chain fatty acids	64:93	C17 branched-chain fatty acids	64:93	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	6	10	theme	fatty	1067:1071	arg1	acids					1073:1077	the most abundant branched-chain fatty acids	1034:1077	the most abundant branched-chain fatty acids in milk fat	1034:1089	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	6	10	theme	fatty	1067:1071	arg1	15:0					987:990	Iso 15:0	983:990	Iso 15:0	983:990	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	7	11	theme	17:0	1215:1218	arg1	17:0					1239:1242	anteiso 17:0	1231:1242	anteiso 17:0 in milk fat	1231:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	11	theme	17:0	1215:1218	arg1	%					1206:1206	49%	1204:1206	49% of iso 17:0	1204:1218	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	11	theme	17:0	1215:1218	arg1	17:0					1215:1218	iso 17:0	1211:1218	iso 17:0	1211:1218	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	11	theme	17:0	1215:1218	arg1	%					1226:1226	60%	1224:1226	60% of anteiso 17:0 in milk fat	1224:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	6	12	theme	milk	1082:1085	arg1	fat					1087:1089	milk fat	1082:1089	milk fat	1082:1089	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	5	13	from	starch	869:874	arg1	rich					861:864	rich	861:864	rich	861:864	Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil.					
31722762	0	14	theme	branched-chain	68:81	arg1	acids					89:93	C17 branched-chain fatty acids	64:93	C17 branched-chain fatty acids	64:93	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	5	15	theme	oil	978:980	arg1	g/d					963:965	30 g/d	960:965	30 g/d of linseed oil	960:980	Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil.					
31722762	5	16	theme	Experimental	794:805	arg1	diets					807:811	Experimental diets	794:811	Experimental diets	794:811	Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil.					
31722762	3	17	with	design	599:604	arg1	periods					659:665	two experimental periods	642:665	two experimental periods of 25 d	642:673	Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d.					
31722762	3	17	with	design	599:604	arg1	animals					616:622	four animals	611:622	four animals per treatment	611:636	Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d.					
31722762	7	18	theme	17:0	1239:1242	arg1	17:0					1239:1242	anteiso 17:0	1231:1242	anteiso 17:0 in milk fat	1231:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	18	theme	17:0	1239:1242	arg1	%					1206:1206	49%	1204:1206	49% of iso 17:0	1204:1218	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	18	theme	17:0	1239:1242	arg1	17:0					1215:1218	iso 17:0	1211:1218	iso 17:0	1211:1218	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	18	theme	17:0	1239:1242	arg1	%					1226:1226	60%	1224:1226	60% of anteiso 17:0 in milk fat	1224:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	2	19	theme	putative	455:462	arg1	precursors					464:473	their putative precursors iso 15:0 and anteiso 15:0	449:499	their putative precursors iso 15:0 and anteiso 15:0	449:499	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	5	20	with	combination	943:953	arg1	g/d					963:965	30 g/d	960:965	30 g/d of linseed oil	960:980	Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil.					
31722762	6	21	theme	gas	1111:1113	arg1	chromatography					1115:1128	gas chromatography	1111:1128	gas chromatography using two different capillary columns	1111:1166	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	4	22	from	experiments	684:694	arg1	sole					717:720	sole	717:720	sole	717:720	In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant.					
31722762	4	22	from	experiments	684:694	arg1	hay					705:707	alfalfa hay	697:707	alfalfa hay	697:707	In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant.					
31722762	7	23	theme	milk	1247:1250	arg1	fat					1252:1254	milk fat	1247:1254	milk fat	1247:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	24	from	%	1206:1206	arg1	fat					1252:1254	milk fat	1247:1254	milk fat	1247:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	0	25	theme	acids	89:93	arg1	origin					54:59	the origin	50:59	the origin of C17 branched-chain fatty acids in caprine milk fat	50:113	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	3	26	theme	experimental	646:657	arg1	periods					659:665	two experimental periods	642:665	two experimental periods of 25 d	642:673	Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d.					
31722762	4	27	theme	alfalfa	697:703	arg1	sole					717:720	sole	717:720	sole	717:720	In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant.					
31722762	4	27	theme	alfalfa	697:703	arg1	hay					705:707	alfalfa hay	697:707	alfalfa hay	697:707	In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant.					
31722762	4	28	dep	sole	717:720	arg1	forage					722:727	forage	722:727	forage	722:727	In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant.					
31722762	6	29	theme	anteiso	1020:1026	arg1	17:0					1028:1031	anteiso 17:0	1020:1031	anteiso 17:0	1020:1031	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	2	30	dep	confirm	293:299	arg1	to					290:291	to	290:291	to	290:291	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	5	31	from	fibre	897:901	arg1	rich					861:864	rich	861:864	rich	861:864	Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil.					
31722762	7	32	theme	regression	1173:1182	arg1	model					1184:1188	The regression model	1169:1188	The regression model	1169:1188	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	2	33	theme	17:0	397:400	arg1	proportions					361:371	the proportions	357:371	the proportions of iso 17:0 and anteiso 17:0 in milk fat	357:412	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	7	34	theme	iso	1211:1213	arg1	17:0					1215:1218	iso 17:0	1211:1218	iso 17:0	1211:1218	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	0	35	theme	linear	17:22	arg1	model					35:39	a linear regression model	15:39	a linear regression model	15:39	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	1	36	theme	endogenous	239:248	arg1	synthesis					270:278	endogenous extraruminal tissue synthesis	239:278	endogenous extraruminal tissue synthesis	239:278	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	0	37	theme	milk	106:109	arg1	fat					111:113	caprine milk fat	98:113	caprine milk fat	98:113	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	2	38	theme	17:0	380:383	arg1	proportions					361:371	the proportions	357:371	the proportions of iso 17:0 and anteiso 17:0 in milk fat	357:412	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	4	39	from	sole	717:720	arg1	experiments					684:694	both experiments	679:694	both experiments	679:694	In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant.					
31722762	1	40	theme	extraruminal	250:261	arg1	synthesis					270:278	endogenous extraruminal tissue synthesis	239:278	endogenous extraruminal tissue synthesis	239:278	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	0	41	theme	caprine	98:104	arg1	fat					111:113	caprine milk fat	98:113	caprine milk fat	98:113	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	6	42	theme	iso	1007:1009	arg1	17:0					1011:1014	iso 17:0	1007:1014	iso 17:0	1007:1014	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	2	43	theme	iso	376:378	arg1	17:0					380:383	iso 17:0	376:383	iso 17:0	376:383	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	0	44	theme	model	35:39	arg1	Application					0:10	Application	0:10	Application of a linear regression model	0:39	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	1	45	theme	tissue	263:268	arg1	synthesis					270:278	endogenous extraruminal tissue synthesis	239:278	endogenous extraruminal tissue synthesis	239:278	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	3	46	theme	crossover	589:597	arg1	design					599:604	a crossover design	587:604	a crossover design with four animals per treatment and two experimental periods of 25 d	587:673	Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d.					
31722762	7	47	from	%	1226:1226	arg1	fat					1252:1254	milk fat	1247:1254	milk fat	1247:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	1	48	theme	iso	185:187	arg1	17:0					189:192	iso 17:0	185:192	iso 17:0	185:192	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	0	49	theme	regression	24:33	arg1	model					35:39	a linear regression model	15:39	a linear regression model	15:39	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	1	50	from	fat	219:221	arg1	part					177:180	a part	175:180	a part of iso 17:0 and anteiso 17:0 in milk fat	175:221	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	0	51	from	origin	54:59	arg1	fat					111:113	caprine milk fat	98:113	caprine milk fat	98:113	Application of a linear regression model to study the origin of C17 branched-chain fatty acids in caprine milk fat.					
31722762	2	52	theme	anteiso	389:395	arg1	17:0					397:400	anteiso 17:0	389:400	anteiso 17:0	389:400	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	1	53	theme	17:0	189:192	arg1	part					177:180	a part	175:180	a part of iso 17:0 and anteiso 17:0 in milk fat	175:221	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	2	54	theme	iso	475:477	arg1	15:0					479:482	iso 15:0	475:482	iso 15:0	475:482	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	1	55	from	17:0	206:209	arg1	fat					219:221	milk fat	214:221	milk fat	214:221	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	5	56	theme	linseed	970:976	arg1	oil					978:980	linseed oil	970:980	linseed oil	970:980	Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil.					
31722762	3	57	theme	dairy	524:528	arg1	goats					530:534	Sixteen dairy goats	516:534	Sixteen dairy goats	516:534	Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d.					
31722762	1	58	from	17:0	189:192	arg1	fat					219:221	milk fat	214:221	milk fat	214:221	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	2	59	theme	milk	405:408	arg1	fat					410:412	milk fat	405:412	milk fat	405:412	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	2	60	dep	come	425:428	arg1	could					419:423	could	419:423	could	419:423	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	1	61	from	part	177:180	arg1	fat					219:221	milk fat	214:221	milk fat	214:221	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	7	62	theme	anteiso	1300:1306	arg1	elongation					1313:1322	anteiso 15:0 elongation	1300:1322	anteiso 15:0 elongation	1300:1322	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	1	63	dep	come	229:232	arg1	could					223:227	could	223:227	could	223:227	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	3	64	theme	simultaneous	558:569	arg1	experiments					571:581	two simultaneous experiments	554:581	two simultaneous experiments	554:581	Sixteen dairy goats were allocated to two simultaneous experiments, in a crossover design with four animals per treatment and two experimental periods of 25 d.					
31722762	6	65	theme	anteiso	993:999	arg1	15:0					1001:1004	anteiso 15:0	993:1004	anteiso 15:0	993:1004	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	5	66	from	rich	861:864	arg1	starch					869:874	starch	869:874	starch	869:874	Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil.					
31722762	5	66	from	rich	861:864	arg1	fibre					897:901	fibre	897:901	fibre	897:901	Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil.					
31722762	7	67	theme	anteiso	1231:1237	arg1	17:0					1239:1242	anteiso 17:0	1231:1242	anteiso 17:0 in milk fat	1231:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	6	68	theme	capillary	1150:1158	arg1	columns					1160:1166	two different capillary columns	1136:1166	two different capillary columns	1136:1166	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	4	69	dep	the	733:735	arg1	concentrate					747:757	concentrate	747:757	to concentrate ratio (33 : 67)	744:773	In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant.					
31722762	4	69	dep	the	733:735	arg1	forage					737:742	forage	737:742	forage	737:742	In both experiments, alfalfa hay was the sole forage and the forage to concentrate ratio (33 : 67) remained constant.					
31722762	2	70	dep	precursors	464:473	arg1	15:0					479:482	iso 15:0	475:482	iso 15:0	475:482	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	2	70	dep	precursors	464:473	arg1	15:0					496:499	anteiso 15:0	488:499	anteiso 15:0	488:499	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	5	71	theme	concentrate	829:839	arg1	composition					841:851	the concentrate composition	825:851	the concentrate composition	825:851	Experimental diets differed on the concentrate composition, either rich in starch or neutral detergent fibre, and they were administered alone or in combination with 30 g/d of linseed oil.					
31722762	7	72	from	17:0	1239:1242	arg1	fat					1252:1254	milk fat	1247:1254	milk fat	1247:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	1	73	theme	anteiso	198:204	arg1	17:0					206:209	anteiso 17:0	198:209	anteiso 17:0	198:209	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	6	74	theme	Iso	983:985	arg1	acids					1073:1077	the most abundant branched-chain fatty acids	1034:1077	the most abundant branched-chain fatty acids in milk fat	1034:1089	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	6	74	theme	Iso	983:985	arg1	15:0					987:990	Iso 15:0	983:990	Iso 15:0	983:990	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	6	75	theme	branched-chain	1052:1065	arg1	acids					1073:1077	the most abundant branched-chain fatty acids	1034:1077	the most abundant branched-chain fatty acids in milk fat	1034:1089	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	6	75	theme	branched-chain	1052:1065	arg1	15:0					987:990	Iso 15:0	983:990	Iso 15:0	983:990	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	2	76	from	proportions	361:371	arg1	fat					410:412	milk fat	405:412	milk fat	405:412	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
31722762	1	77	theme	17:0	206:209	arg1	part					177:180	a part	175:180	a part of iso 17:0 and anteiso 17:0 in milk fat	175:221	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	7	78	theme	15:0	1308:1311	arg1	elongation					1313:1322	anteiso 15:0 elongation	1300:1322	anteiso 15:0 elongation	1300:1322	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	6	79	theme	abundant	1043:1050	arg1	acids					1073:1077	the most abundant branched-chain fatty acids	1034:1077	the most abundant branched-chain fatty acids in milk fat	1034:1089	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	6	79	theme	abundant	1043:1050	arg1	15:0					987:990	Iso 15:0	983:990	Iso 15:0	983:990	Iso 15:0, anteiso 15:0, iso 17:0 and anteiso 17:0, the most abundant branched-chain fatty acids in milk fat, were determined by gas chromatography using two different capillary columns.					
31722762	1	80	theme	research	121:128	arg1	communications					130:143	This research communications	116:143	This research communications	116:143	This research communications addresses the hypothesis that a part of iso 17:0 and anteiso 17:0 in milk fat could come from endogenous extraruminal tissue synthesis.					
31722762	7	81	from	fat	1252:1254	arg1	17:0					1239:1242	anteiso 17:0	1231:1242	anteiso 17:0 in milk fat	1231:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	81	from	fat	1252:1254	arg1	%					1206:1206	49%	1204:1206	49% of iso 17:0	1204:1218	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	81	from	fat	1252:1254	arg1	17:0					1215:1218	iso 17:0	1211:1218	iso 17:0	1211:1218	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	7	81	from	fat	1252:1254	arg1	%					1226:1226	60%	1224:1226	60% of anteiso 17:0 in milk fat	1224:1254	The regression model resolved that 49% of iso 17:0 and 60% of anteiso 17:0 in milk fat was formed extraruminally from iso 15:0 and anteiso 15:0 elongation.					
31722762	2	82	theme	precursors	464:473	arg1	elongation					435:444	elongation	435:444	elongation of their putative precursors iso 15:0 and anteiso 15:0	435:499	In order to confirm this a linear regression model was applied to calculate the proportions of iso 17:0 and anteiso 17:0 in milk fat that could come from elongation of their putative precursors iso 15:0 and anteiso 15:0, respectively.					
30413726	0	0	theme	comparative	94:104	arg1	approach					115:122	comparative genomics approach	94:122	comparative genomics approach	94:122	Unlocking conserved and diverged metabolic characteristics in cassava carbon assimilation via comparative genomics approach.					
30413726	5	1	from	metabolites	1030:1040	arg1	sub-metabolisms					1051:1065	eight sub-metabolisms	1045:1065	eight sub-metabolisms	1045:1065	MeRecon, available at http://bml.sbi.kmutt.ac.th/MeRecon, comprises 259 reactions (199 EC numbers), 1,052 proteins (870 genes) and 259 metabolites in eight sub-metabolisms.					
30413726	6	2	theme	biochemical	1291:1301	arg1	reaction					1303:1310	each biochemical reaction	1286:1310	each biochemical reaction	1286:1310	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	3	3	theme	other	535:539	arg1	crops					548:552	other starch crops	535:552	other starch crops	535:552	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	1	4	theme	atmospheric	267:277	arg1	carbon					279:284	harvested atmospheric carbon	257:284	harvested atmospheric carbon	257:284	Globally, cassava is an important source of starch, which is synthesized through carbon assimilation in cellular metabolism whereby harvested atmospheric carbon is assimilated into macromolecules.					
30413726	6	5	theme	functions	1381:1389	arg1	numbers					1321:1327	numbers	1321:1327	numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype	1321:1444	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	1	6	theme	starch	169:174	arg1	source					159:164	an important source	146:164	an important source	146:164	Globally, cassava is an important source of starch, which is synthesized through carbon assimilation in cellular metabolism whereby harvested atmospheric carbon is assimilated into macromolecules.					
30413726	1	6	theme	starch	169:174	arg1	cassava					135:141	cassava	135:141	cassava	135:141	Globally, cassava is an important source of starch, which is synthesized through carbon assimilation in cellular metabolism whereby harvested atmospheric carbon is assimilated into macromolecules.					
30413726	4	7	dep	templates	825:833	arg1	bean					869:872	castor bean	862:872	castor bean	862:872	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	7	dep	templates	825:833	arg1	rice					849:852	rice	849:852	rice	849:852	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	7	dep	templates	825:833	arg1	potato					875:880	potato	875:880	potato	875:880	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	7	dep	templates	825:833	arg1	templates					825:833	six plant templates	815:833	six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip	815:892	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	7	dep	templates	825:833	arg1	maize					855:859	maize	855:859	maize	855:859	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	7	dep	templates	825:833	arg1	turnip					887:892	turnip	887:892	turnip	887:892	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	7	dep	templates	825:833	arg1	Arabidopsis					836:846	Arabidopsis	836:846	Arabidopsis	836:846	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	6	8	theme	evolved	1373:1379	arg1	functions					1381:1389	their evolved functions	1367:1389	their evolved functions	1367:1389	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	5	9	from	http	917:920	arg1	available					904:912	available	904:912	available	904:912	MeRecon, available at http://bml.sbi.kmutt.ac.th/MeRecon, comprises 259 reactions (199 EC numbers), 1,052 proteins (870 genes) and 259 metabolites in eight sub-metabolisms.					
30413726	5	10	theme	EC	982:983	arg1	numbers					985:991	199 EC numbers	978:991	199 EC numbers	978:991	MeRecon, available at http://bml.sbi.kmutt.ac.th/MeRecon, comprises 259 reactions (199 EC numbers), 1,052 proteins (870 genes) and 259 metabolites in eight sub-metabolisms.					
30413726	5	10	theme	EC	982:983	arg1	reactions					967:975	259 reactions	963:975	259 reactions (199 EC numbers)	963:992	MeRecon, available at http://bml.sbi.kmutt.ac.th/MeRecon, comprises 259 reactions (199 EC numbers), 1,052 proteins (870 genes) and 259 metabolites in eight sub-metabolisms.					
30413726	6	11	theme	templates	1138:1146	arg1	pathways					1116:1123	the carbon assimilation pathways	1092:1123	the carbon assimilation pathways of the plant templates	1092:1146	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	6	11	theme	templates	1138:1146	arg1	MeRecon					1080:1086	MeRecon	1080:1086	MeRecon	1080:1086	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	7	12	theme	phenotypes	1571:1580	arg1	synthesis					1548:1556	the synthesis	1544:1556	the synthesis of metabolic phenotypes of cassava	1544:1591	Thus, this study provides insights into the network characteristics and mechanisms that regulate the synthesis of metabolic phenotypes of cassava.					
30413726	0	13	theme	genomics	106:113	arg1	approach					115:122	comparative genomics approach	94:122	comparative genomics approach	94:122	Unlocking conserved and diverged metabolic characteristics in cassava carbon assimilation via comparative genomics approach.					
30413726	3	14	theme	starch	567:572	arg1	production					574:583	starch production	567:583	starch production	567:583	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	6	15	theme	pathways	1116:1123	arg1	Analysis					1068:1075	Analysis	1068:1075	Analysis of MeRecon and the carbon assimilation pathways of the plant templates	1068:1146	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	4	16	theme	assimilation	754:765	arg1	pathway					767:773	the carbon assimilation pathway	743:773	the carbon assimilation pathway	743:773	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	6	17	theme	MeRecon	1080:1086	arg1	Analysis					1068:1075	Analysis	1068:1075	Analysis of MeRecon and the carbon assimilation pathways of the plant templates	1068:1146	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	6	18	theme	proteins	1354:1361	arg1	numbers					1321:1327	numbers	1321:1327	numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype	1321:1444	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	0	19	from	characteristics	43:57	arg1	assimilation					77:88	cassava carbon assimilation	62:88	cassava carbon assimilation	62:88	Unlocking conserved and diverged metabolic characteristics in cassava carbon assimilation via comparative genomics approach.					
30413726	6	20	theme	metabolic	1426:1434	arg1	phenotype					1436:1444	the distinct metabolic phenotype	1413:1444	the distinct metabolic phenotype	1413:1444	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	7	21	theme	cassava	1585:1591	arg1	phenotypes					1571:1580	metabolic phenotypes	1561:1580	metabolic phenotypes of cassava	1561:1591	Thus, this study provides insights into the network characteristics and mechanisms that regulate the synthesis of metabolic phenotypes of cassava.					
30413726	4	22	theme	plant	819:823	arg1	bean					869:872	castor bean	862:872	castor bean	862:872	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	22	theme	plant	819:823	arg1	rice					849:852	rice	849:852	rice	849:852	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	22	theme	plant	819:823	arg1	potato					875:880	potato	875:880	potato	875:880	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	22	theme	plant	819:823	arg1	templates					825:833	six plant templates	815:833	six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip	815:892	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	22	theme	plant	819:823	arg1	maize					855:859	maize	855:859	maize	855:859	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	22	theme	plant	819:823	arg1	turnip					887:892	turnip	887:892	turnip	887:892	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	22	theme	plant	819:823	arg1	Arabidopsis					836:846	Arabidopsis	836:846	Arabidopsis	836:846	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	6	23	located	found	1243:1247	arg1	relation					1252:1259	relation	1252:1259	relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype	1252:1444	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	6	23	located	found	1243:1247	arg2	variations					1203:1212	variations	1203:1212	variations at sub metabolism level	1203:1236	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	6	24	theme	overall	1161:1167	arg1	topology					1169:1176	the overall topology	1157:1176	the overall topology	1157:1176	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	5	25	from	proteins	1001:1008	arg1	sub-metabolisms					1051:1065	eight sub-metabolisms	1045:1065	eight sub-metabolisms	1045:1065	MeRecon, available at http://bml.sbi.kmutt.ac.th/MeRecon, comprises 259 reactions (199 EC numbers), 1,052 proteins (870 genes) and 259 metabolites in eight sub-metabolisms.					
30413726	2	26	theme	assimilation	342:353	arg1	pathway					355:361	the carbon assimilation pathway	331:361	the carbon assimilation pathway	331:361	Although the carbon assimilation pathway is highly conserved across species, metabolic phenotypes could differ in composition, type, and quantity.					
30413726	7	27	theme	metabolic	1561:1569	arg1	phenotypes					1571:1580	metabolic phenotypes	1561:1580	metabolic phenotypes of cassava	1561:1591	Thus, this study provides insights into the network characteristics and mechanisms that regulate the synthesis of metabolic phenotypes of cassava.					
30413726	6	28	theme	sub	1217:1219	arg1	level					1232:1236	sub metabolism level	1217:1236	sub metabolism level	1217:1236	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	3	29	theme	production	574:583	arg1	terms					558:562	terms	558:562	terms of starch production	558:583	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	4	30	theme	cassava	778:784	arg1	MeRecon					733:739	MeRecon	733:739	MeRecon	733:739	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	6	31	theme	responsible	1332:1342	arg1	proteins					1354:1361	responsible enzymatic proteins	1332:1361	responsible enzymatic proteins	1332:1361	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	3	32	theme	starch	541:546	arg1	crops					548:552	other starch crops	535:552	other starch crops	535:552	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	1	33	theme	carbon	206:211	arg1	assimilation					213:224	carbon assimilation	206:224	carbon assimilation	206:224	Globally, cassava is an important source of starch, which is synthesized through carbon assimilation in cellular metabolism whereby harvested atmospheric carbon is assimilated into macromolecules.					
30413726	6	34	theme	assimilation	1103:1114	arg1	pathways					1116:1123	the carbon assimilation pathways	1092:1123	the carbon assimilation pathways of the plant templates	1092:1146	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	7	35	dep	characteristics	1499:1513	arg1	the					1487:1489	the	1487:1489	the	1487:1489	Thus, this study provides insights into the network characteristics and mechanisms that regulate the synthesis of metabolic phenotypes of cassava.					
30413726	6	36	theme	metabolism	1221:1230	arg1	level					1232:1236	sub metabolism level	1217:1236	sub metabolism level	1217:1236	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	3	37	theme	comparative	696:706	arg1	analysis					716:723	comparative network analysis	696:723	comparative network analysis	696:723	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	3	38	theme	assimilation	613:624	arg1	mechanisms					626:635	the carbon assimilation mechanisms	602:635	the carbon assimilation mechanisms in cassava	602:646	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	3	39	from	mechanisms	626:635	arg1	cassava					640:646	cassava	640:646	cassava	640:646	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	0	40	theme	metabolic	33:41	arg1	characteristics					43:57	metabolic characteristics	33:57	metabolic characteristics in cassava carbon assimilation	33:88	Unlocking conserved and diverged metabolic characteristics in cassava carbon assimilation via comparative genomics approach.					
30413726	6	41	theme	distinct	1417:1424	arg1	phenotype					1436:1444	the distinct metabolic phenotype	1413:1444	the distinct metabolic phenotype	1413:1444	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	3	42	theme	pathway	669:675	arg1	reconstruction					677:690	genome-based pathway reconstruction	656:690	genome-based pathway reconstruction	656:690	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	3	43	theme	metabolic	484:492	arg1	complexity					494:503	the metabolic complexity	480:503	the metabolic complexity	480:503	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	0	44	theme	cassava	62:68	arg1	assimilation					77:88	cassava carbon assimilation	62:88	cassava carbon assimilation	62:88	Unlocking conserved and diverged metabolic characteristics in cassava carbon assimilation via comparative genomics approach.					
30413726	4	45	theme	castor	862:867	arg1	bean					869:872	castor bean	862:872	castor bean	862:872	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	4	45	theme	castor	862:867	arg1	templates					825:833	six plant templates	815:833	six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip	815:892	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	5	46	dep	http	917:920	arg1	//bml.sbi.kmutt.ac.th/MeRecon					922:950	//bml.sbi.kmutt.ac.th/MeRecon	922:950	http://bml.sbi.kmutt.ac.th/MeRecon	917:950	MeRecon, available at http://bml.sbi.kmutt.ac.th/MeRecon, comprises 259 reactions (199 EC numbers), 1,052 proteins (870 genes) and 259 metabolites in eight sub-metabolisms.					
30413726	3	47	theme	cassava	522:528	arg1	advantage					509:517	advantage	509:517	advantage of cassava over other starch crops	509:552	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	3	47	theme	cassava	522:528	arg1	complexity					494:503	the metabolic complexity	480:503	the metabolic complexity	480:503	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	6	48	dep	responsible	1332:1342	arg1	enzymatic					1344:1352	enzymatic	1344:1352	enzymatic	1344:1352	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	3	49	theme	carbon	606:611	arg1	mechanisms					626:635	the carbon assimilation mechanisms	602:635	the carbon assimilation mechanisms in cassava	602:646	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	4	50	theme	carbon	747:752	arg1	pathway					767:773	the carbon assimilation pathway	743:773	the carbon assimilation pathway	743:773	First, MeRecon - the carbon assimilation pathway of cassava was reconstructed based upon six plant templates: Arabidopsis, rice, maize, castor bean, potato, and turnip.					
30413726	2	51	theme	carbon	335:340	arg1	pathway					355:361	the carbon assimilation pathway	331:361	the carbon assimilation pathway	331:361	Although the carbon assimilation pathway is highly conserved across species, metabolic phenotypes could differ in composition, type, and quantity.					
30413726	1	52	theme	cellular	229:236	arg1	metabolism					238:247	cellular metabolism	229:247	cellular metabolism whereby harvested atmospheric carbon is assimilated into macromolecules	229:319	Globally, cassava is an important source of starch, which is synthesized through carbon assimilation in cellular metabolism whereby harvested atmospheric carbon is assimilated into macromolecules.					
30413726	3	53	theme	network	708:714	arg1	analysis					716:723	comparative network analysis	696:723	comparative network analysis	696:723	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	3	54	theme	genome-based	656:667	arg1	reconstruction					677:690	genome-based pathway reconstruction	656:690	genome-based pathway reconstruction	656:690	To unravel the metabolic complexity and advantage of cassava over other starch crops, in terms of starch production, we investigated the carbon assimilation mechanisms in cassava through genome-based pathway reconstruction and comparative network analysis.					
30413726	5	55	theme	available	904:912	arg1	MeRecon					895:901	MeRecon	895:901	MeRecon	895:901	MeRecon, available at http://bml.sbi.kmutt.ac.th/MeRecon, comprises 259 reactions (199 EC numbers), 1,052 proteins (870 genes) and 259 metabolites in eight sub-metabolisms.					
30413726	6	56	theme	plant	1132:1136	arg1	templates					1138:1146	the plant templates	1128:1146	the plant templates	1128:1146	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	6	57	from	level	1232:1236	arg1	variations					1203:1212	variations	1203:1212	variations at sub metabolism level	1203:1236	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	6	58	theme	carbon	1096:1101	arg1	pathways					1116:1123	the carbon assimilation pathways	1092:1123	the carbon assimilation pathways of the plant templates	1092:1146	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	1	59	theme	important	149:157	arg1	source					159:164	an important source	146:164	an important source	146:164	Globally, cassava is an important source of starch, which is synthesized through carbon assimilation in cellular metabolism whereby harvested atmospheric carbon is assimilated into macromolecules.					
30413726	1	59	theme	important	149:157	arg1	cassava					135:141	cassava	135:141	cassava	135:141	Globally, cassava is an important source of starch, which is synthesized through carbon assimilation in cellular metabolism whereby harvested atmospheric carbon is assimilated into macromolecules.					
30413726	5	60	from	reactions	967:975	arg1	sub-metabolisms					1051:1065	eight sub-metabolisms	1045:1065	eight sub-metabolisms	1045:1065	MeRecon, available at http://bml.sbi.kmutt.ac.th/MeRecon, comprises 259 reactions (199 EC numbers), 1,052 proteins (870 genes) and 259 metabolites in eight sub-metabolisms.					
30413726	0	61	theme	carbon	70:75	arg1	assimilation					77:88	cassava carbon assimilation	62:88	cassava carbon assimilation	62:88	Unlocking conserved and diverged metabolic characteristics in cassava carbon assimilation via comparative genomics approach.					
30413726	7	62	theme	network	1491:1497	arg1	characteristics					1499:1513	network characteristics	1491:1513	network characteristics	1491:1513	Thus, this study provides insights into the network characteristics and mechanisms that regulate the synthesis of metabolic phenotypes of cassava.					
30413726	6	63	dep	such	1313:1316	arg1	as					1318:1319	as	1318:1319	as	1318:1319	Analysis of MeRecon and the carbon assimilation pathways of the plant templates revealed the overall topology is highly conserved, but variations at sub metabolism level were found in relation to complexity underlying each biochemical reaction, such as numbers of responsible enzymatic proteins and their evolved functions, which likely explain the distinct metabolic phenotype.					
30413726	2	64	theme	metabolic	399:407	arg1	phenotypes					409:418	metabolic phenotypes	399:418	metabolic phenotypes	399:418	Although the carbon assimilation pathway is highly conserved across species, metabolic phenotypes could differ in composition, type, and quantity.					
30413726	1	65	theme	harvested	257:265	arg1	carbon					279:284	harvested atmospheric carbon	257:284	harvested atmospheric carbon	257:284	Globally, cassava is an important source of starch, which is synthesized through carbon assimilation in cellular metabolism whereby harvested atmospheric carbon is assimilated into macromolecules.					
29414307	9	0	theme	paste	1540:1544	arg1	A					1556:1556	the commercial paste Omicilon® A	1525:1556	the commercial paste Omicilon® A	1525:1556	of microemulsion application was much higher than after overnight application of the commercial paste Omicilon® A.					
29414307	6	1	theme	parameters	1020:1029	arg1	determination					988:1000	The determination	984:1000	The determination of the permeation parameters	984:1029	The determination of the permeation parameters allowed us to show that chitosan acts on the diffusion parameter across the tissue and not on the partitioning parameter; for the same reason the tissue retention of triamcinolone acetonide was not modified.					
29414307	10	2	theme	buccal	1706:1711	arg1	mucosa					1713:1718	the buccal mucosa	1702:1718	the buccal mucosa	1702:1718	This suggests that triamcinolone acetonide microemulsions can be an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa.					
29414307	1	3	theme	buccal	163:168	arg1	administration					170:183	the buccal administration	159:183	the buccal administration of triamcinolone acetonide	159:210	The aim of the present work was to investigate the potential of microemulsions for the buccal administration of triamcinolone acetonide.					
29414307	6	4	theme	permeation	1009:1018	arg1	parameters					1020:1029	the permeation parameters	1005:1029	the permeation parameters	1005:1029	The determination of the permeation parameters allowed us to show that chitosan acts on the diffusion parameter across the tissue and not on the partitioning parameter; for the same reason the tissue retention of triamcinolone acetonide was not modified.					
29414307	5	5	theme	triamcinolone	742:754	arg1	acetonide					756:764	triamcinolone acetonide	742:764	triamcinolone acetonide	742:764	The results obtained show that the permeation of triamcinolone acetonide across pig esophageal epithelium was not influenced by the droplet size nor by the composition, but only by the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia.					
29414307	6	6	theme	acetonide	1211:1219	arg1	retention					1184:1192	the tissue retention	1173:1192	the tissue retention of triamcinolone acetonide	1173:1219	The determination of the permeation parameters allowed us to show that chitosan acts on the diffusion parameter across the tissue and not on the partitioning parameter; for the same reason the tissue retention of triamcinolone acetonide was not modified.					
29414307	2	7	theme	titration	314:322	arg1	method					324:329	the aqueous titration method	302:329	the aqueous titration method	302:329	Microemulsions were developed by the construction of pseudoternary phase diagrams, using the aqueous titration method.					
29414307	5	8	theme	stratified	962:971	arg1	epithelia					973:981	stratified epithelia	962:981	stratified epithelia	962:981	The results obtained show that the permeation of triamcinolone acetonide across pig esophageal epithelium was not influenced by the droplet size nor by the composition, but only by the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia.					
29414307	5	9	theme	acetonide	756:764	arg1	permeation					728:737	the permeation	724:737	the permeation of triamcinolone acetonide across pig esophageal epithelium	724:797	The results obtained show that the permeation of triamcinolone acetonide across pig esophageal epithelium was not influenced by the droplet size nor by the composition, but only by the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia.					
29414307	6	10	theme	tissue	1177:1182	arg1	retention					1184:1192	the tissue retention	1173:1192	the tissue retention of triamcinolone acetonide	1173:1219	The determination of the permeation parameters allowed us to show that chitosan acts on the diffusion parameter across the tissue and not on the partitioning parameter; for the same reason the tissue retention of triamcinolone acetonide was not modified.					
29414307	2	11	theme	aqueous	306:312	arg1	method					324:329	the aqueous titration method	302:329	the aqueous titration method	302:329	Microemulsions were developed by the construction of pseudoternary phase diagrams, using the aqueous titration method.					
29414307	10	12	theme	triamcinolone	1578:1590	arg1	acetonide					1592:1600	triamcinolone acetonide	1578:1600	triamcinolone acetonide microemulsions	1578:1615	This suggests that triamcinolone acetonide microemulsions can be an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa.					
29414307	4	13	theme	able	655:658	arg1	stearylamine					622:633	stearylamine	622:633	stearylamine	622:633	Furthermore, one microemulsion was added of excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets.					
29414307	4	13	theme	able	655:658	arg1	excipients					610:619	excipients	610:619	excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets	610:690	Furthermore, one microemulsion was added of excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets.					
29414307	4	13	theme	able	655:658	arg1	chitosan					645:652	chitosan	645:652	chitosan	645:652	Furthermore, one microemulsion was added of excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets.					
29414307	4	13	theme	able	655:658	arg1	CTAB					636:639	CTAB	636:639	CTAB	636:639	Furthermore, one microemulsion was added of excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets.					
29414307	6	14	theme	diffusion	1076:1084	arg1	parameter					1086:1094	the diffusion parameter	1072:1094	the diffusion parameter across the tissue	1072:1112	The determination of the permeation parameters allowed us to show that chitosan acts on the diffusion parameter across the tissue and not on the partitioning parameter; for the same reason the tissue retention of triamcinolone acetonide was not modified.					
29414307	5	15	theme	pig	773:775	arg1	epithelium					788:797	pig esophageal epithelium	773:797	pig esophageal epithelium	773:797	The results obtained show that the permeation of triamcinolone acetonide across pig esophageal epithelium was not influenced by the droplet size nor by the composition, but only by the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia.					
29414307	10	16	theme	commercial	1658:1667	arg1	formulation					1669:1679	the commercial formulation to treat diseases of the buccal mucosa	1654:1718	the commercial formulation to treat diseases of the buccal mucosa	1654:1718	This suggests that triamcinolone acetonide microemulsions can be an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa.					
29414307	7	17	theme	feasible	1320:1327	arg1	administration					1305:1318	systemic administration	1296:1318	systemic administration feasible	1296:1327	Triamcinolone flux (2.6 μg cm-2 h-1) was too low to make systemic administration feasible (dose required 2.5 to 60 mg/day).					
29414307	9	18	theme	overnight	1500:1508	arg1	application					1510:1520	overnight application	1500:1520	overnight application of the commercial paste Omicilon® A	1500:1556	of microemulsion application was much higher than after overnight application of the commercial paste Omicilon® A.					
29414307	10	19	theme	interesting	1627:1637	arg1	alternative					1639:1649	an interesting alternative	1624:1649	an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa	1624:1718	This suggests that triamcinolone acetonide microemulsions can be an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa.					
29414307	10	19	theme	interesting	1627:1637	arg1	microemulsions					1602:1615	triamcinolone acetonide microemulsions	1578:1615	triamcinolone acetonide microemulsions	1578:1615	This suggests that triamcinolone acetonide microemulsions can be an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa.					
29414307	6	20	theme	same	1161:1164	arg1	reason					1166:1171	the same reason	1157:1171	the same reason	1157:1171	The determination of the permeation parameters allowed us to show that chitosan acts on the diffusion parameter across the tissue and not on the partitioning parameter; for the same reason the tissue retention of triamcinolone acetonide was not modified.					
29414307	1	21	theme	present	91:97	arg1	work					99:102	the present work	87:102	the present work	87:102	The aim of the present work was to investigate the potential of microemulsions for the buccal administration of triamcinolone acetonide.					
29414307	1	22	theme	triamcinolone	188:200	arg1	acetonide					202:210	triamcinolone acetonide	188:210	triamcinolone acetonide	188:210	The aim of the present work was to investigate the potential of microemulsions for the buccal administration of triamcinolone acetonide.					
29414307	3	23	dep	in	450:451	arg1	vitro					453:457	vitro	453:457	vitro	453:457	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	1	24	theme	work	99:102	arg1	aim					80:82	The aim	76:82	The aim of the present work	76:102	The aim of the present work was to investigate the potential of microemulsions for the buccal administration of triamcinolone acetonide.					
29414307	3	25	theme	permeation/retention	459:478	arg1	experiments					480:490	in vitro permeation/retention experiments	450:490	in vitro permeation/retention experiments	450:490	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	6	26	theme	triamcinolone	1197:1209	arg1	acetonide					1211:1219	triamcinolone acetonide	1197:1219	triamcinolone acetonide	1197:1219	The determination of the permeation parameters allowed us to show that chitosan acts on the diffusion parameter across the tissue and not on the partitioning parameter; for the same reason the tissue retention of triamcinolone acetonide was not modified.					
29414307	3	27	dep	microemulsions	342:355	arg1	tested					370:375	tested	370:375	tested for stability	370:389	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	3	27	dep	microemulsions	342:355	arg1	prepared					357:364	prepared	357:364	prepared	357:364	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	8	28	theme	triamcinolone	1377:1389	arg1	acetonide					1391:1399	triamcinolone acetonide	1377:1399	triamcinolone acetonide	1377:1399	The amount of triamcinolone acetonide recovered in the mucosa after only 10 min.					
29414307	3	29	theme	esophageal	503:512	arg1	model					538:542	an accepted model	526:542	an accepted model of the buccal mucosa	526:563	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	3	29	theme	esophageal	503:512	arg1	epithelium					514:523	pig esophageal epithelium	499:523	pig esophageal epithelium	499:523	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	0	30	theme	triamcinolone	25:37	arg1	acetonide					39:47	triamcinolone acetonide	25:47	triamcinolone acetonide for buccal administration	25:73	Microemulsion containing triamcinolone acetonide for buccal administration.					
29414307	5	31	theme	able	915:918	arg1	polysaccharide					900:913	polysaccharide	900:913	polysaccharide able to increase the transport across mono and stratified epithelia	900:981	The results obtained show that the permeation of triamcinolone acetonide across pig esophageal epithelium was not influenced by the droplet size nor by the composition, but only by the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia.					
29414307	5	31	theme	able	915:918	arg1	chitosan					890:897	chitosan	890:897	chitosan	890:897	The results obtained show that the permeation of triamcinolone acetonide across pig esophageal epithelium was not influenced by the droplet size nor by the composition, but only by the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia.					
29414307	9	32	theme	Omicilon®	1546:1554	arg1	A					1556:1556	the commercial paste Omicilon® A	1525:1556	the commercial paste Omicilon® A	1525:1556	of microemulsion application was much higher than after overnight application of the commercial paste Omicilon® A.					
29414307	1	33	theme	microemulsions	140:153	arg1	potential					127:135	the potential	123:135	the potential of microemulsions for the buccal administration of triamcinolone acetonide	123:210	The aim of the present work was to investigate the potential of microemulsions for the buccal administration of triamcinolone acetonide.					
29414307	0	34	theme	buccal	53:58	arg1	administration					60:73	buccal administration	53:73	buccal administration	53:73	Microemulsion containing triamcinolone acetonide for buccal administration.					
29414307	6	35	theme	partitioning	1129:1140	arg1	parameter					1142:1150	the partitioning parameter	1125:1150	the partitioning parameter	1125:1150	The determination of the permeation parameters allowed us to show that chitosan acts on the diffusion parameter across the tissue and not on the partitioning parameter; for the same reason the tissue retention of triamcinolone acetonide was not modified.					
29414307	1	36	theme	acetonide	202:210	arg1	administration					170:183	the buccal administration	159:183	the buccal administration of triamcinolone acetonide	159:210	The aim of the present work was to investigate the potential of microemulsions for the buccal administration of triamcinolone acetonide.					
29414307	3	37	theme	in	450:451	arg1	experiments					480:490	in vitro permeation/retention experiments	450:490	in vitro permeation/retention experiments	450:490	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	8	38	theme	acetonide	1391:1399	arg1	amount					1367:1372	The amount	1363:1372	The amount of triamcinolone acetonide	1363:1399	The amount of triamcinolone acetonide recovered in the mucosa after only 10 min.					
29414307	8	38	theme	acetonide	1391:1399	arg1	acetonide					1391:1399	triamcinolone acetonide	1377:1399	triamcinolone acetonide	1377:1399	The amount of triamcinolone acetonide recovered in the mucosa after only 10 min.					
29414307	3	39	theme	pig	499:501	arg1	model					538:542	an accepted model	526:542	an accepted model of the buccal mucosa	526:563	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	3	39	theme	pig	499:501	arg1	epithelium					514:523	pig esophageal epithelium	499:523	pig esophageal epithelium	499:523	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	5	40	theme	chitosan	890:897	arg1	presence					878:885	the presence	874:885	the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia	874:981	The results obtained show that the permeation of triamcinolone acetonide across pig esophageal epithelium was not influenced by the droplet size nor by the composition, but only by the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia.					
29414307	4	41	dep	excipients	610:619	arg1	stearylamine					622:633	stearylamine	622:633	stearylamine	622:633	Furthermore, one microemulsion was added of excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets.					
29414307	4	41	dep	excipients	610:619	arg1	excipients					610:619	excipients	610:619	excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets	610:690	Furthermore, one microemulsion was added of excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets.					
29414307	4	41	dep	excipients	610:619	arg1	chitosan					645:652	chitosan	645:652	chitosan	645:652	Furthermore, one microemulsion was added of excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets.					
29414307	4	41	dep	excipients	610:619	arg1	CTAB					636:639	CTAB	636:639	CTAB	636:639	Furthermore, one microemulsion was added of excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets.					
29414307	3	42	theme	accepted	529:536	arg1	model					538:542	an accepted model	526:542	an accepted model of the buccal mucosa	526:563	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	3	42	theme	accepted	529:536	arg1	epithelium					514:523	pig esophageal epithelium	499:523	pig esophageal epithelium	499:523	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	5	43	theme	droplet	825:831	arg1	size					833:836	the droplet size	821:836	the droplet size	821:836	The results obtained show that the permeation of triamcinolone acetonide across pig esophageal epithelium was not influenced by the droplet size nor by the composition, but only by the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia.					
29414307	9	44	theme	A	1556:1556	arg1	application					1510:1520	overnight application	1500:1520	overnight application of the commercial paste Omicilon® A	1500:1556	of microemulsion application was much higher than after overnight application of the commercial paste Omicilon® A.					
29414307	4	45	theme	droplets	683:690	arg1	charge					673:678	the charge	669:678	the charge of droplets	669:690	Furthermore, one microemulsion was added of excipients (stearylamine, CTAB and chitosan) able to alter the charge of droplets.					
29414307	8	46	theme	only	1431:1434	arg1	10 min					1436:1441	only 10 min	1431:1441	only 10 min	1431:1441	The amount of triamcinolone acetonide recovered in the mucosa after only 10 min.					
29414307	9	47	theme	commercial	1529:1538	arg1	A					1556:1556	the commercial paste Omicilon® A	1525:1556	the commercial paste Omicilon® A	1525:1556	of microemulsion application was much higher than after overnight application of the commercial paste Omicilon® A.					
29414307	11	48	used	used	1784:1787	arg2	formulation					1765:1775	the formulation	1761:1775	the formulation	1761:1775	Owing to the fast uptake by the tissue, the formulation can be used as a mouthwash.					
29414307	11	48	used	used	1784:1787	arg2	mouthwash					1794:1802	a mouthwash	1792:1802	a mouthwash	1792:1802	Owing to the fast uptake by the tissue, the formulation can be used as a mouthwash.					
29414307	11	49	theme	fast	1734:1737	arg1	uptake					1739:1744	the fast uptake	1730:1744	the fast uptake by the tissue	1730:1758	Owing to the fast uptake by the tissue, the formulation can be used as a mouthwash.					
29414307	2	50	theme	diagrams	286:293	arg1	construction					250:261	the construction	246:261	the construction of pseudoternary phase diagrams	246:293	Microemulsions were developed by the construction of pseudoternary phase diagrams, using the aqueous titration method.					
29414307	7	51	dep	60 mg/day	1351:1359	arg1	to					1348:1349	to	1348:1349	to	1348:1349	Triamcinolone flux (2.6 μg cm-2 h-1) was too low to make systemic administration feasible (dose required 2.5 to 60 mg/day).					
29414307	9	52	theme	microemulsion	1447:1459	arg1	application					1461:1471	microemulsion application	1447:1471	microemulsion application	1447:1471	of microemulsion application was much higher than after overnight application of the commercial paste Omicilon® A.					
29414307	5	53	theme	esophageal	777:786	arg1	epithelium					788:797	pig esophageal epithelium	773:797	pig esophageal epithelium	773:797	The results obtained show that the permeation of triamcinolone acetonide across pig esophageal epithelium was not influenced by the droplet size nor by the composition, but only by the presence of chitosan, polysaccharide able to increase the transport across mono and stratified epithelia.					
29414307	7	54	theme	systemic	1296:1303	arg1	administration					1305:1318	systemic administration	1296:1318	systemic administration feasible	1296:1327	Triamcinolone flux (2.6 μg cm-2 h-1) was too low to make systemic administration feasible (dose required 2.5 to 60 mg/day).					
29414307	2	55	theme	phase	280:284	arg1	diagrams					286:293	pseudoternary phase diagrams	266:293	pseudoternary phase diagrams	266:293	Microemulsions were developed by the construction of pseudoternary phase diagrams, using the aqueous titration method.					
29414307	10	56	theme	mucosa	1713:1718	arg1	diseases					1690:1697	diseases	1690:1697	diseases of the buccal mucosa	1690:1718	This suggests that triamcinolone acetonide microemulsions can be an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa.					
29414307	3	57	theme	buccal	551:556	arg1	mucosa					558:563	the buccal mucosa	547:563	the buccal mucosa	547:563	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	7	58	theme	Triamcinolone	1239:1251	arg1	2.6 μg cm-2 h-1					1259:1273	2.6 μg cm-2 h-1	1259:1273	2.6 μg cm-2 h-1	1259:1273	Triamcinolone flux (2.6 μg cm-2 h-1) was too low to make systemic administration feasible (dose required 2.5 to 60 mg/day).					
29414307	7	58	theme	Triamcinolone	1239:1251	arg1	flux					1253:1256	Triamcinolone flux	1239:1256	Triamcinolone flux (2.6 μg cm-2 h-1)	1239:1274	Triamcinolone flux (2.6 μg cm-2 h-1) was too low to make systemic administration feasible (dose required 2.5 to 60 mg/day).					
29414307	2	59	theme	pseudoternary	266:278	arg1	diagrams					286:293	pseudoternary phase diagrams	266:293	pseudoternary phase diagrams	266:293	Microemulsions were developed by the construction of pseudoternary phase diagrams, using the aqueous titration method.					
29414307	3	60	theme	mucosa	558:563	arg1	model					538:542	an accepted model	526:542	an accepted model of the buccal mucosa	526:563	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	3	60	theme	mucosa	558:563	arg1	epithelium					514:523	pig esophageal epithelium	499:523	pig esophageal epithelium	499:523	Among all microemulsions prepared and tested for stability, three were selected and submitted to characterization and in vitro permeation/retention experiments, using pig esophageal epithelium, an accepted model of the buccal mucosa.					
29414307	10	61	theme	acetonide	1592:1600	arg1	alternative					1639:1649	an interesting alternative	1624:1649	an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa	1624:1718	This suggests that triamcinolone acetonide microemulsions can be an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa.					
29414307	10	61	theme	acetonide	1592:1600	arg1	microemulsions					1602:1615	triamcinolone acetonide microemulsions	1578:1615	triamcinolone acetonide microemulsions	1578:1615	This suggests that triamcinolone acetonide microemulsions can be an interesting alternative to the commercial formulation to treat diseases of the buccal mucosa.					
30423717	0	0	theme	tissue	115:120	arg1	engineering					122:132	cartilage tissue engineering	105:132	cartilage tissue engineering	105:132	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides microspheres for cartilage tissue engineering.					
30423717	4	1	theme	PAPs	537:540	arg1	microspheres					548:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	Then, the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres was prepared, and their compatibility was studied.					
30423717	8	2	theme	cartilage	1026:1034	arg1	repair					1036:1041	articular cartilage repair	1016:1041	articular cartilage repair	1016:1041	The defects were almost completely repaired after 13 weeks in the PAP-SF/COL/HA group, thereby indicating that the PAP-SF/COL/HA composite had a favorable effect on articular cartilage repair.					
30423717	6	3	theme	rabbit	679:684	arg1	model					703:707	A rabbit cartilage defect model	677:707	A rabbit cartilage defect model	677:707	A rabbit cartilage defect model was established.					
30423717	0	4	theme	cartilage	105:113	arg1	engineering					122:132	cartilage tissue engineering	105:132	cartilage tissue engineering	105:132	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides microspheres for cartilage tissue engineering.					
30423717	5	5	theme	favorable	639:647	arg1	adhesion					649:656	favorable adhesion	639:656	favorable adhesion	639:656	PAP-SF/COL/HA scaffold had favorable adhesion and proliferation.					
30423717	7	6	theme	cartilage	747:755	arg1	defects					757:763	cartilage defects	747:763	cartilage defects	747:763	The repair effect of cartilage defects was observed and evaluated among PAP-SF/COL/HA, SF/COL/HA, and sham operation groups.					
30423717	8	7	theme	PAP-SF/COL/HA	917:929	arg1	group					931:935	the PAP-SF/COL/HA group	913:935	the PAP-SF/COL/HA group	913:935	The defects were almost completely repaired after 13 weeks in the PAP-SF/COL/HA group, thereby indicating that the PAP-SF/COL/HA composite had a favorable effect on articular cartilage repair.					
30423717	4	8	theme	-PLGA	542:546	arg1	microspheres					548:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	Then, the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres was prepared, and their compatibility was studied.					
30423717	1	9	theme	silk	137:140	arg1	scaffold					197:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold was prepared via admixing, crosslinking, and lyophilizing processes.					
30423717	4	10	theme	antler	516:521	arg1	microspheres					548:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	Then, the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres was prepared, and their compatibility was studied.					
30423717	4	11	theme	pilose	509:514	arg1	microspheres					548:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	Then, the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres was prepared, and their compatibility was studied.					
30423717	0	12	theme	Silk	0:3	arg1	scaffold					38:45	Silk fibroin/collagen/hyaluronic acid scaffold	0:45	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides	0:86	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides microspheres for cartilage tissue engineering.					
30423717	0	13	theme	fibroin/collagen/hyaluronic	5:31	arg1	scaffold					38:45	Silk fibroin/collagen/hyaluronic acid scaffold	0:45	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides	0:86	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides microspheres for cartilage tissue engineering.					
30423717	4	14	theme	polypeptides	523:534	arg1	microspheres					548:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	pilose antler polypeptides (PAPs)-PLGA microspheres	509:559	Then, the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres was prepared, and their compatibility was studied.					
30423717	5	15	theme	PAP-SF/COL/HA	612:624	arg1	scaffold					626:633	PAP-SF/COL/HA scaffold	612:633	PAP-SF/COL/HA scaffold	612:633	PAP-SF/COL/HA scaffold had favorable adhesion and proliferation.					
30423717	3	16	theme	SF/COL/HA	425:433	arg1	scaffold					435:442	SF/COL/HA scaffold	425:442	SF/COL/HA scaffold	425:442	The optimal ratio of SF/COL/HA scaffold was 3:6.5:0.5.					
30423717	3	17	theme	optimal	408:414	arg1	ratio					416:420	The optimal ratio	404:420	The optimal ratio of SF/COL/HA scaffold	404:442	The optimal ratio of SF/COL/HA scaffold was 3:6.5:0.5.					
30423717	5	18	contain	had	635:637	arg1	scaffold					626:633	PAP-SF/COL/HA scaffold	612:633	PAP-SF/COL/HA scaffold	612:633	PAP-SF/COL/HA scaffold had favorable adhesion and proliferation.					
30423717	5	18	contain	had	635:637	arg2	proliferation					662:674	proliferation	662:674	proliferation	662:674	PAP-SF/COL/HA scaffold had favorable adhesion and proliferation.					
30423717	5	18	contain	had	635:637	arg2	adhesion					649:656	favorable adhesion	639:656	favorable adhesion	639:656	PAP-SF/COL/HA scaffold had favorable adhesion and proliferation.					
30423717	0	19	theme	acid	33:36	arg1	scaffold					38:45	Silk fibroin/collagen/hyaluronic acid scaffold	0:45	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides	0:86	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides microspheres for cartilage tissue engineering.					
30423717	7	20	theme	defects	757:763	arg1	effect					737:742	The repair effect	726:742	The repair effect of cartilage defects	726:763	The repair effect of cartilage defects was observed and evaluated among PAP-SF/COL/HA, SF/COL/HA, and sham operation groups.					
30423717	2	21	theme	water	341:345	arg1	absorption					347:356	water absorption	341:356	water absorption	341:356	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	0	22	theme	pilose	61:66	arg1	polypeptides					75:86	pilose antler polypeptides	61:86	pilose antler polypeptides	61:86	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides microspheres for cartilage tissue engineering.					
30423717	8	23	theme	PAP-SF/COL/HA	966:978	arg1	composite					980:988	the PAP-SF/COL/HA composite	962:988	the PAP-SF/COL/HA composite	962:988	The defects were almost completely repaired after 13 weeks in the PAP-SF/COL/HA group, thereby indicating that the PAP-SF/COL/HA composite had a favorable effect on articular cartilage repair.					
30423717	7	24	theme	operation	833:841	arg1	groups					843:848	PAP-SF/COL/HA, SF/COL/HA, and sham operation groups	798:848	PAP-SF/COL/HA, SF/COL/HA, and sham operation groups	798:848	The repair effect of cartilage defects was observed and evaluated among PAP-SF/COL/HA, SF/COL/HA, and sham operation groups.					
30423717	1	25	theme	lyophilizing	251:262	arg1	processes					264:272	lyophilizing processes	251:272	lyophilizing processes	251:272	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold was prepared via admixing, crosslinking, and lyophilizing processes.					
30423717	3	26	theme	scaffold	435:442	arg1	ratio					416:420	The optimal ratio	404:420	The optimal ratio of SF/COL/HA scaffold	404:442	The optimal ratio of SF/COL/HA scaffold was 3:6.5:0.5.					
30423717	4	27	theme	scaffold	486:493	arg1	ratio					477:481	the optimal ratio	465:481	the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres	465:559	Then, the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres was prepared, and their compatibility was studied.					
30423717	1	28	theme	fibroin/collagen/hyaluronic	142:168	arg1	scaffold					197:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold was prepared via admixing, crosslinking, and lyophilizing processes.					
30423717	0	29	theme	antler	68:73	arg1	polypeptides					75:86	pilose antler polypeptides	61:86	pilose antler polypeptides	61:86	Silk fibroin/collagen/hyaluronic acid scaffold incorporating pilose antler polypeptides microspheres for cartilage tissue engineering.					
30423717	8	30	contain	had	990:992	arg2	effect					1006:1011	a favorable effect	994:1011	a favorable effect	994:1011	The defects were almost completely repaired after 13 weeks in the PAP-SF/COL/HA group, thereby indicating that the PAP-SF/COL/HA composite had a favorable effect on articular cartilage repair.					
30423717	8	30	contain	had	990:992	arg1	composite					980:988	the PAP-SF/COL/HA composite	962:988	the PAP-SF/COL/HA composite	962:988	The defects were almost completely repaired after 13 weeks in the PAP-SF/COL/HA group, thereby indicating that the PAP-SF/COL/HA composite had a favorable effect on articular cartilage repair.					
30423717	7	31	theme	repair	730:735	arg1	effect					737:742	The repair effect	726:742	The repair effect of cartilage defects	726:763	The repair effect of cartilage defects was observed and evaluated among PAP-SF/COL/HA, SF/COL/HA, and sham operation groups.					
30423717	1	32	theme	acid	170:173	arg1	scaffold					197:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold was prepared via admixing, crosslinking, and lyophilizing processes.					
30423717	7	33	theme	sham	828:831	arg1	operation					833:841	sham operation	828:841	sham operation	828:841	The repair effect of cartilage defects was observed and evaluated among PAP-SF/COL/HA, SF/COL/HA, and sham operation groups.					
30423717	2	34	theme	biological	310:319	arg1	loss					376:379	weight loss	369:379	weight loss	369:379	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	2	34	theme	biological	310:319	arg1	biocompatibility					386:401	biocompatibility	386:401	biocompatibility	386:401	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	2	34	theme	biological	310:319	arg1	properties					321:330	its physicochemical and biological properties	286:330	its physicochemical and biological properties	286:330	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	2	34	theme	biological	310:319	arg1	porosity					359:366	porosity	359:366	porosity	359:366	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	2	34	theme	biological	310:319	arg1	absorption					347:356	water absorption	341:356	water absorption	341:356	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	8	35	theme	favorable	996:1004	arg1	effect					1006:1011	a favorable effect	994:1011	a favorable effect	994:1011	The defects were almost completely repaired after 13 weeks in the PAP-SF/COL/HA group, thereby indicating that the PAP-SF/COL/HA composite had a favorable effect on articular cartilage repair.					
30423717	2	36	theme	weight	369:374	arg1	loss					376:379	weight loss	369:379	weight loss	369:379	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	7	37	theme	SF/COL/HA	813:821	arg1	groups					843:848	PAP-SF/COL/HA, SF/COL/HA, and sham operation groups	798:848	PAP-SF/COL/HA, SF/COL/HA, and sham operation groups	798:848	The repair effect of cartilage defects was observed and evaluated among PAP-SF/COL/HA, SF/COL/HA, and sham operation groups.					
30423717	4	38	theme	optimal	469:475	arg1	ratio					477:481	the optimal ratio	465:481	the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres	465:559	Then, the optimal ratio of scaffold incorporating pilose antler polypeptides (PAPs)-PLGA microspheres was prepared, and their compatibility was studied.					
30423717	8	39	theme	articular	1016:1024	arg1	repair					1036:1041	articular cartilage repair	1016:1041	articular cartilage repair	1016:1041	The defects were almost completely repaired after 13 weeks in the PAP-SF/COL/HA group, thereby indicating that the PAP-SF/COL/HA composite had a favorable effect on articular cartilage repair.					
30423717	1	40	theme	SF/COL/HA	176:184	arg1	scaffold					197:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold was prepared via admixing, crosslinking, and lyophilizing processes.					
30423717	2	41	theme	physicochemical	290:304	arg1	loss					376:379	weight loss	369:379	weight loss	369:379	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	2	41	theme	physicochemical	290:304	arg1	biocompatibility					386:401	biocompatibility	386:401	biocompatibility	386:401	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	2	41	theme	physicochemical	290:304	arg1	properties					321:330	its physicochemical and biological properties	286:330	its physicochemical and biological properties	286:330	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	2	41	theme	physicochemical	290:304	arg1	porosity					359:366	porosity	359:366	porosity	359:366	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	2	41	theme	physicochemical	290:304	arg1	absorption					347:356	water absorption	341:356	water absorption	341:356	We studied its physicochemical and biological properties, such as water absorption, porosity, weight loss, and biocompatibility.					
30423717	6	42	theme	defect	696:701	arg1	model					703:707	A rabbit cartilage defect model	677:707	A rabbit cartilage defect model	677:707	A rabbit cartilage defect model was established.					
30423717	7	43	theme	PAP-SF/COL/HA	798:810	arg1	groups					843:848	PAP-SF/COL/HA, SF/COL/HA, and sham operation groups	798:848	PAP-SF/COL/HA, SF/COL/HA, and sham operation groups	798:848	The repair effect of cartilage defects was observed and evaluated among PAP-SF/COL/HA, SF/COL/HA, and sham operation groups.					
30423717	1	44	theme	composite	187:195	arg1	scaffold					197:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold	135:204	A silk fibroin/collagen/hyaluronic acid (SF/COL/HA) composite scaffold was prepared via admixing, crosslinking, and lyophilizing processes.					
30423717	6	45	theme	cartilage	686:694	arg1	model					703:707	A rabbit cartilage defect model	677:707	A rabbit cartilage defect model	677:707	A rabbit cartilage defect model was established.					
30590183	10	0	theme	spatial	1723:1729	arg1	organization					1731:1742	spatial organization	1723:1742	spatial organization	1723:1742	With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment.					
30590183	14	1	theme	cartilage	2536:2544	arg1	matrix					2546:2551	dense cartilage matrix	2530:2551	dense cartilage matrix	2530:2551	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	11	2	theme	clinical	2152:2159	arg1	outcome					2161:2167	inconsistent clinical outcome	2139:2167	inconsistent clinical outcome	2139:2167	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	6	3	with	tests	953:957	arg1	hAC					994:996	hAC	994:996	hAC	994:996	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	6	3	with	tests	953:957	arg1	chondrocytes					980:991	human articular chondrocytes	964:991	human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC)	964:1073	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	14	4	theme	novel	2445:2449	arg1	approach					2501:2508	the first approach	2491:2508	the first approach enabling the use of dense cartilage matrix	2491:2551	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	14	4	theme	novel	2445:2449	arg1	scaffold					2451:2458	The novel scaffold	2441:2458	The novel scaffold developed within this study	2441:2486	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	10	5	theme	optimal	1767:1773	arg1	environment					1788:1798	an optimal chondrogenic environment	1764:1798	an optimal chondrogenic environment for therapeutic cells	1764:1820	With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment.					
30590183	3	6	with	repopulation	663:674	arg1	cells					693:697	therapeutic cells	681:697	therapeutic cells	681:697	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	1	7	theme	repair	261:266	arg1	tissue					268:273	repair tissue	261:273	repair tissue	261:273	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	13	8	theme	used	2338:2341	arg1	biomaterials					2343:2354	the currently used biomaterials	2324:2354	the currently used biomaterials	2324:2354	Considering the currently used biomaterials, structure and mechanical properties appear to be insufficient to fulfill this task.					
30590183	11	9	theme	graft	2185:2189	arg1	failures					2191:2198	early and late graft failures	2170:2198	early and late graft failures	2170:2198	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	16	10	theme	future	2858:2863	arg1	approaches					2888:2897	future cartilage regeneration approaches	2858:2897	future cartilage regeneration approaches	2858:2897	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	6	11	link	adipose-derived	1034:1048	arg1	cells					1063:1067	human adipose-derived stromal/stem cells	1028:1067	human adipose-derived stromal/stem cells (ASC)	1028:1073	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	6	11	link	adipose-derived	1034:1048	arg1	ASC					1070:1072	ASC	1070:1072	ASC	1070:1072	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	5	12	theme	compressive	806:816	arg1	scaffold					912:919	diminished, but three times higher than in the clinically used collagen type I/III scaffold	829:919	diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control	829:942	The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control.					
30590183	5	12	theme	compressive	806:816	arg1	modulus					818:824	The compressive modulus	802:824	The compressive modulus	802:824	The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control.					
30590183	16	13	theme	regeneration	2875:2886	arg1	approaches					2888:2897	future cartilage regeneration approaches	2858:2897	future cartilage regeneration approaches	2858:2897	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	11	14	dep	outcome	2161:2167	arg1	failures					2191:2198	early and late graft failures	2170:2198	early and late graft failures	2170:2198	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	3	15	theme	channel	577:583	arg1	network					585:591	a traversing channel network	564:591	a traversing channel network	564:591	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	13	16	theme	mechanical	2371:2380	arg1	properties					2382:2391	mechanical properties	2371:2391	mechanical properties	2371:2391	Considering the currently used biomaterials, structure and mechanical properties appear to be insufficient to fulfill this task.					
30590183	6	17	theme	Seeding	945:951	arg1	tests					953:957	Seeding tests	945:957	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC)	945:1073	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	2	18	theme	cartilage	534:542	arg1	formation					500:508	the formation	496:508	the formation of high-quality hyaline cartilage	496:542	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	8	19	theme	compact	1437:1443	arg1	type					1454:1457	compact collagen type II	1437:1460	compact collagen type II matrix	1437:1467	In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels.					
30590183	10	20	theme	cartilage	1831:1839	arg1	defects					1841:1847	cartilage defects	1831:1847	cartilage defects	1831:1847	With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment.					
30590183	1	21	theme	hyaline	214:220	arg1	cartilage					222:230	hyaline cartilage	214:230	hyaline cartilage	214:230	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	8	22	theme	type	1454:1457	arg1	matrix					1462:1467	compact collagen type II matrix	1437:1467	compact collagen type II matrix	1437:1467	In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels.					
30590183	3	23	theme	elastic	630:636	arg1	fibers					638:643	elastic fibers	630:643	elastic fibers	630:643	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	16	24	theme	high	2832:2835	arg1	potential					2837:2845	a high potential	2830:2845	a high potential to improve future cartilage regeneration approaches	2830:2897	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	11	25	theme	tissue	2018:2023	arg1	engineering					2025:2035	tissue engineering	2018:2035	tissue engineering for cartilage regeneration	2018:2062	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	14	26	dep	approach	2501:2508	arg1	provide					2585:2591	provide	2585:2591	provide the cells with a compact collagen type II environment	2585:2645	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	14	26	dep	approach	2501:2508	arg1	repopulate					2554:2563	repopulate	2554:2563	repopulate it via channels	2554:2579	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	4	27	theme	collagen	712:719	arg1	matrix					729:734	The complex collagen type II matrix	700:734	The complex collagen type II matrix	700:734	The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM.					
30590183	4	27	theme	collagen	712:719	arg1	intact					744:749	intact	744:749	intact	744:749	The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM.					
30590183	5	28	theme	collagen	892:899	arg1	I/III					906:910	the clinically used collagen type I/III	872:910	the clinically used collagen type I/III	872:910	The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control.					
30590183	14	29	theme	collagen	2618:2625	arg1	type					2627:2630	a compact collagen type II	2608:2633	a compact collagen type II environment	2608:2645	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	2	30	theme	high-quality	513:524	arg1	cartilage					534:542	high-quality hyaline cartilage	513:542	high-quality hyaline cartilage	513:542	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	12	31	theme	scaffolding	2266:2276	arg1	function					2302:2309	their scaffolding, guiding and protective function	2260:2309	their scaffolding, guiding and protective function	2260:2309	Especially large defects are dependent on biomaterials and their scaffolding, guiding and protective function.					
30590183	0	32	theme	enzyme	79:84	arg1	combination					86:96	an enzyme combination	76:96	an enzyme combination	76:96	Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.					
30590183	3	33	theme	AuriScaff	545:553	arg1	features					555:562	AuriScaff features	545:562	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.	545:698	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	6	34	theme	articular	970:978	arg1	hAC					994:996	hAC	994:996	hAC	994:996	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	6	34	theme	articular	970:978	arg1	chondrocytes					980:991	human articular chondrocytes	964:991	human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC)	964:1073	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	11	35	theme	SIGNIFICANCE	1983:1994	arg1	STATEMENT					1970:1978	STATEMENT	1970:1978	STATEMENT OF SIGNIFICANCE	1970:1994	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	2	36	theme	perforated	376:385	arg1	"					356:356	"AuriScaff"	346:356	"AuriScaff"	346:356	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	2	36	theme	perforated	376:385	arg1	scaffold					414:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	7	37	theme	collagen	1326:1333	arg1	orientation					1311:1321	the orientation	1307:1321	the orientation of collagen within articular cartilage	1307:1360	It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage.					
30590183	2	38	theme	novel	429:433	arg1	biomaterial					435:445	a novel biomaterial	427:445	a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage	427:542	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	7	39	theme	collagen	1165:1172	arg1	alignment					1174:1182	collagen alignment	1165:1182	collagen alignment along the channels	1165:1201	It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage.					
30590183	1	40	theme	tissue	268:273	arg1	formation					248:256	the formation	244:256	the formation of repair tissue with inferior characteristics	244:303	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	6	41	theme	stromal/stem	1050:1061	arg1	cells					1063:1067	human adipose-derived stromal/stem cells	1028:1067	human adipose-derived stromal/stem cells (ASC)	1028:1073	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	6	41	theme	stromal/stem	1050:1061	arg1	ASC					1070:1072	ASC	1070:1072	ASC	1070:1072	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	8	42	theme	plug	1383:1386	arg1	model					1388:1392	an osteochondral plug model	1366:1392	an osteochondral plug model	1366:1392	In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels.					
30590183	1	43	theme	inferior	280:287	arg1	characteristics					289:303	inferior characteristics	280:303	inferior characteristics	280:303	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	3	44	theme	fibers	638:643	arg1	depletion					617:625	selective depletion	607:625	selective depletion of elastic fibers	607:643	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	6	45	theme	human	1028:1032	arg1	cells					1063:1067	human adipose-derived stromal/stem cells	1028:1067	human adipose-derived stromal/stem cells (ASC)	1028:1073	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	6	45	theme	human	1028:1032	arg1	ASC					1070:1072	ASC	1070:1072	ASC	1070:1072	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	3	46	theme	uniform	655:661	arg1	repopulation					663:674	uniform repopulation	655:674	uniform repopulation with therapeutic cells	655:697	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	2	47	theme	auricular	394:402	arg1	"					356:356	"AuriScaff"	346:356	"AuriScaff"	346:356	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	2	47	theme	auricular	394:402	arg1	scaffold					414:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	0	48	theme	cartilage	29:37	arg1	scaffold					39:46	an auricular cartilage scaffold	16:46	an auricular cartilage scaffold	16:46	Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.					
30590183	0	48	theme	cartilage	29:37	arg1	AuriScaff					49:57	AuriScaff	49:57	AuriScaff	49:57	Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.					
30590183	9	49	theme	deep	1608:1611	arg1	defects					1613:1619	even deep defects	1603:1619	even deep defects	1603:1619	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	6	50	theme	cell	1110:1113	arg1	migration					1115:1123	cell migration	1110:1123	cell migration	1110:1123	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	8	51	theme	complete	1416:1423	arg1	defect					1425:1430	the complete defect	1412:1430	the complete defect	1412:1430	In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels.					
30590183	1	52	from	Biomaterials	99:110	arg1	use					125:127	use	125:127	use for articular cartilage	125:151	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	1	53	theme	cartilage	222:230	arg1	architecture					198:209	architecture	198:209	architecture	198:209	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	1	53	theme	cartilage	222:230	arg1	composition					183:193	composition	183:193	composition	183:193	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	10	54	theme	channel-guided	1897:1910	arg1	repopulation					1912:1923	channel-guided repopulation	1897:1923	channel-guided repopulation followed by matrix deposition and alignment	1897:1967	With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment.					
30590183	3	55	dep	features	555:562	arg1	enabling					646:653	enabling	646:653	enabling uniform repopulation with therapeutic cells	646:697	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	2	56	theme	"	356:356	arg1	use					339:341	the use	335:341	the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage	335:542	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	9	57	theme	hyaline-like	1639:1650	arg1	cartilage					1652:1660	high-quality hyaline-like cartilage	1626:1660	high-quality hyaline-like cartilage	1626:1660	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	7	58	theme	channel	1238:1244	arg1	alignment					1246:1254	the generally traverse channel alignment	1215:1254	the generally traverse channel alignment	1215:1254	It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage.					
30590183	14	59	theme	matrix	2546:2551	arg1	use					2523:2525	the use	2519:2525	the use of dense cartilage matrix	2519:2551	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	11	60	theme	early	2170:2174	arg1	failures					2191:2198	early and late graft failures	2170:2198	early and late graft failures	2170:2198	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	10	61	theme	matrix	1937:1942	arg1	deposition					1944:1953	matrix deposition	1937:1953	matrix deposition	1937:1953	With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment.					
30590183	10	62	theme	chondrogenic	1775:1786	arg1	environment					1788:1798	an optimal chondrogenic environment	1764:1798	an optimal chondrogenic environment for therapeutic cells	1764:1820	With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment.					
30590183	14	63	theme	dense	2530:2534	arg1	matrix					2546:2551	dense cartilage matrix	2530:2551	dense cartilage matrix	2530:2551	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	9	64	theme	adult	1537:1541	arg1	chondrocytes					1553:1564	adult articular chondrocytes	1537:1564	adult articular chondrocytes from bovine origin (bAC)	1537:1589	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	9	65	with	filling	1592:1598	arg1	cartilage					1652:1660	high-quality hyaline-like cartilage	1626:1660	high-quality hyaline-like cartilage	1626:1660	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	11	66	theme	optimal	2083:2089	arg1	strategy					2091:2098	no optimal strategy	2080:2098	no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures	2080:2198	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	11	67	theme	cartilage	2041:2049	arg1	regeneration					2051:2062	cartilage regeneration	2041:2062	cartilage regeneration	2041:2062	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	15	68	theme	better	2685:2690	arg1	properties					2703:2712	better mechanical properties	2685:2712	better mechanical properties	2685:2712	Due to its density, it also provides better mechanical properties than materials currently used in clinics.					
30590183	3	69	theme	traversing	566:575	arg1	network					585:591	a traversing channel network	564:591	a traversing channel network	564:591	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	6	70	theme	human	964:968	arg1	hAC					994:996	hAC	994:996	hAC	994:996	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	6	70	theme	human	964:968	arg1	chondrocytes					980:991	human articular chondrocytes	964:991	human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC)	964:1073	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	11	71	theme	late	2180:2183	arg1	failures					2191:2198	early and late graft failures	2170:2198	early and late graft failures	2170:2198	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	16	72	theme	cartilage	2865:2873	arg1	approaches					2888:2897	future cartilage regeneration approaches	2858:2897	future cartilage regeneration approaches	2858:2897	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	5	73	theme	diminished	829:838	arg1	modulus					818:824	The compressive modulus	802:824	The compressive modulus	802:824	The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control.					
30590183	5	73	theme	diminished	829:838	arg1	scaffold					912:919	diminished, but three times higher than in the clinically used collagen type I/III scaffold	829:919	diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control	829:942	The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control.					
30590183	10	74	theme	therapeutic	1804:1814	arg1	cells					1816:1820	therapeutic cells	1804:1820	therapeutic cells	1804:1820	With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment.					
30590183	8	75	theme	collagen	1445:1452	arg1	type					1454:1457	compact collagen type II	1437:1460	compact collagen type II matrix	1437:1467	In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels.					
30590183	9	76	theme	articular	1543:1551	arg1	chondrocytes					1553:1564	adult articular chondrocytes	1537:1564	adult articular chondrocytes from bovine origin (bAC)	1537:1589	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	7	77	theme	deposited	1263:1271	arg1	matrix					1283:1288	newly deposited cartilage matrix	1257:1288	newly deposited cartilage matrix	1257:1288	It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage.					
30590183	16	78	theme	auricular	2785:2793	arg1	AuriScaff					2815:2823	AuriScaff	2815:2823	AuriScaff	2815:2823	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	16	78	theme	auricular	2785:2793	arg1	scaffold					2805:2812	the auricular cartilage scaffold	2781:2812	the auricular cartilage scaffold (AuriScaff)	2781:2824	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	2	79	theme	hyaline	526:532	arg1	cartilage					534:542	high-quality hyaline cartilage	513:542	high-quality hyaline cartilage	513:542	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	16	80	theme	cartilage	2795:2803	arg1	AuriScaff					2815:2823	AuriScaff	2815:2823	AuriScaff	2815:2823	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	16	80	theme	cartilage	2795:2803	arg1	scaffold					2805:2812	the auricular cartilage scaffold	2781:2812	the auricular cartilage scaffold (AuriScaff)	2781:2824	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	5	81	theme	type	901:904	arg1	I/III					906:910	the clinically used collagen type I/III	872:910	the clinically used collagen type I/III	872:910	The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control.					
30590183	11	82	theme	inconsistent	2139:2150	arg1	outcome					2161:2167	inconsistent clinical outcome	2139:2167	inconsistent clinical outcome	2139:2167	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	4	83	theme	type	721:724	arg1	matrix					729:734	The complex collagen type II matrix	700:734	The complex collagen type II matrix	700:734	The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM.					
30590183	4	83	theme	type	721:724	arg1	intact					744:749	intact	744:749	intact	744:749	The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM.					
30590183	11	84	theme	engineering	2025:2035	arg1	decades					2007:2013	two decades	2003:2013	two decades of tissue engineering for cartilage regeneration	2003:2062	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	8	85	theme	chondrogenic	1481:1492	arg1	differentiation					1494:1508	chondrogenic differentiation	1481:1508	chondrogenic differentiation	1481:1508	In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels.					
30590183	3	86	theme	selective	607:615	arg1	depletion					617:625	selective depletion	607:625	selective depletion of elastic fibers	607:643	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	1	87	theme	articular	133:141	arg1	cartilage					143:151	articular cartilage	133:151	articular cartilage	133:151	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	7	88	theme	articular	1342:1350	arg1	cartilage					1352:1360	articular cartilage	1342:1360	articular cartilage	1342:1360	It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage.					
30590183	16	89	contain	has	2826:2828	arg2	potential					2837:2845	a high potential	2830:2845	a high potential to improve future cartilage regeneration approaches	2830:2897	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	16	89	contain	has	2826:2828	arg1	AuriScaff					2815:2823	AuriScaff	2815:2823	AuriScaff	2815:2823	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	16	89	contain	has	2826:2828	arg1	scaffold					2805:2812	the auricular cartilage scaffold	2781:2812	the auricular cartilage scaffold (AuriScaff)	2781:2824	We therefore think, that the auricular cartilage scaffold (AuriScaff) has a high potential to improve future cartilage regeneration approaches.					
30590183	2	90	with	repopulation	451:462	arg1	cells					482:486	regenerative cells	469:486	regenerative cells	469:486	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	5	91	theme	used	887:890	arg1	I/III					906:910	the clinically used collagen type I/III	872:910	the clinically used collagen type I/III	872:910	The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control.					
30590183	9	92	from	origin	1578:1583	arg1	chondrocytes					1553:1564	adult articular chondrocytes	1537:1564	adult articular chondrocytes from bovine origin (bAC)	1537:1589	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	14	93	theme	type	2627:2630	arg1	environment					2635:2645	a compact collagen type II environment	2608:2645	a compact collagen type II environment	2608:2645	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	2	94	theme	regenerative	469:480	arg1	cells					482:486	regenerative cells	469:486	regenerative cells	469:486	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	4	95	theme	complex	704:710	arg1	matrix					729:734	The complex collagen type II matrix	700:734	The complex collagen type II matrix	700:734	The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM.					
30590183	4	95	theme	complex	704:710	arg1	intact					744:749	intact	744:749	intact	744:749	The complex collagen type II matrix is left intact, as observed by immunohistochemistry, SEM and TEM.					
30590183	7	96	dep	alignment	1246:1254	arg1	traverse					1229:1236	traverse	1229:1236	traverse	1229:1236	It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage.					
30590183	14	97	theme	compact	2610:2616	arg1	type					2627:2630	a compact collagen type II	2608:2633	a compact collagen type II environment	2608:2645	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	12	98	theme	guiding	2279:2285	arg1	function					2302:2309	their scaffolding, guiding and protective function	2260:2309	their scaffolding, guiding and protective function	2260:2309	Especially large defects are dependent on biomaterials and their scaffolding, guiding and protective function.					
30590183	15	99	theme	mechanical	2692:2701	arg1	properties					2703:2712	better mechanical properties	2685:2712	better mechanical properties	2685:2712	Due to its density, it also provides better mechanical properties than materials currently used in clinics.					
30590183	9	100	theme	bovine	1571:1576	arg1	bAC					1586:1588	bAC	1586:1588	bAC	1586:1588	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	9	100	theme	bovine	1571:1576	arg1	origin					1578:1583	bovine origin	1571:1583	bovine origin (bAC)	1571:1589	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	12	101	theme	protective	2291:2300	arg1	function					2302:2309	their scaffolding, guiding and protective function	2260:2309	their scaffolding, guiding and protective function	2260:2309	Especially large defects are dependent on biomaterials and their scaffolding, guiding and protective function.					
30590183	0	102	theme	auricular	19:27	arg1	scaffold					39:46	an auricular cartilage scaffold	16:46	an auricular cartilage scaffold	16:46	Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.					
30590183	0	102	theme	auricular	19:27	arg1	AuriScaff					49:57	AuriScaff	49:57	AuriScaff	49:57	Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.					
30590183	6	103	theme	adipose-derived	1034:1048	arg1	cells					1063:1067	human adipose-derived stromal/stem cells	1028:1067	human adipose-derived stromal/stem cells (ASC)	1028:1073	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	6	103	theme	adipose-derived	1034:1048	arg1	ASC					1070:1072	ASC	1070:1072	ASC	1070:1072	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	8	104	theme	osteochondral	1369:1381	arg1	model					1388:1392	an osteochondral plug model	1366:1392	an osteochondral plug model	1366:1392	In an osteochondral plug model, AuriScaff filled the complete defect with compact collagen type II matrix and enabled chondrogenic differentiation inside the channels.					
30590183	2	105	theme	cartilage	404:412	arg1	"					356:356	"AuriScaff"	346:356	"AuriScaff"	346:356	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	2	105	theme	cartilage	404:412	arg1	scaffold					414:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	0	106	theme	scaffold	39:46	arg1	Repopulation					0:11	Repopulation	0:11	Repopulation of an auricular cartilage scaffold, AuriScaff,	0:58	Repopulation of an auricular cartilage scaffold, AuriScaff, perforated with an enzyme combination.					
30590183	7	107	theme	cartilage	1273:1281	arg1	matrix					1283:1288	newly deposited cartilage matrix	1257:1288	newly deposited cartilage matrix	1257:1288	It also guides collagen alignment along the channels and, due to the generally traverse channel alignment, newly deposited cartilage matrix corresponds with the orientation of collagen within articular cartilage.					
30590183	10	108	theme	long-term	1876:1884	arg1	outcome					1886:1892	long-term outcome	1876:1892	long-term outcome	1876:1892	With its composition and spatial organization, AuriScaff provides an optimal chondrogenic environment for therapeutic cells to treat cartilage defects and is expected to improve long-term outcome by channel-guided repopulation followed by matrix deposition and alignment.					
30590183	1	109	dep	composition	183:193	arg1	the					179:181	the	179:181	the	179:181	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	2	110	theme	bovine	387:392	arg1	"					356:356	"AuriScaff"	346:356	"AuriScaff"	346:356	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	2	110	theme	bovine	387:392	arg1	scaffold					414:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	9	111	theme	even	1603:1606	arg1	defects					1613:1619	even deep defects	1603:1619	even deep defects	1603:1619	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	11	112	theme	available	2100:2108	arg1	strategy					2091:2098	no optimal strategy	2080:2098	no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures	2080:2198	STATEMENT OF SIGNIFICANCE: After two decades of tissue engineering for cartilage regeneration, there is still no optimal strategy available to overcome problems such as inconsistent clinical outcome, early and late graft failures.					
30590183	3	113	theme	therapeutic	681:691	arg1	cells					693:697	therapeutic cells	681:697	therapeutic cells	681:697	AuriScaff features a traversing channel network, generated by selective depletion of elastic fibers, enabling uniform repopulation with therapeutic cells.					
30590183	14	114	theme	first	2495:2499	arg1	scaffold					2451:2458	The novel scaffold	2441:2458	The novel scaffold developed within this study	2441:2486	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	14	114	theme	first	2495:2499	arg1	approach					2501:2508	the first approach	2491:2508	the first approach enabling the use of dense cartilage matrix	2491:2551	The novel scaffold developed within this study is the first approach enabling the use of dense cartilage matrix, repopulate it via channels and provide the cells with a compact collagen type II environment.					
30590183	12	115	theme	large	2212:2216	arg1	defects					2218:2224	Especially large defects	2201:2224	Especially large defects	2201:2224	Especially large defects are dependent on biomaterials and their scaffolding, guiding and protective function.					
30590183	6	116	with	co-culture	1012:1021	arg1	cells					1063:1067	human adipose-derived stromal/stem cells	1028:1067	human adipose-derived stromal/stem cells (ASC)	1028:1073	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	6	116	with	co-culture	1012:1021	arg1	ASC					1070:1072	ASC	1070:1072	ASC	1070:1072	Seeding tests with human articular chondrocytes (hAC) alone and in co-culture with human adipose-derived stromal/stem cells (ASC) confirmed that the network enabled cell migration throughout the scaffold.					
30590183	9	117	theme	defects	1613:1619	arg1	filling					1592:1598	filling	1592:1598	filling of even deep defects with high-quality hyaline-like cartilage	1592:1660	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	1	118	with	formation	248:256	arg1	characteristics					289:303	inferior characteristics	280:303	inferior characteristics	280:303	Biomaterials currently in use for articular cartilage regeneration do not mimic the composition or architecture of hyaline cartilage, leading to the formation of repair tissue with inferior characteristics.					
30590183	9	119	theme	high-quality	1626:1637	arg1	cartilage					1652:1660	high-quality hyaline-like cartilage	1626:1660	high-quality hyaline-like cartilage	1626:1660	Using adult articular chondrocytes from bovine origin (bAC), filling of even deep defects with high-quality hyaline-like cartilage was achieved after 6 weeks in vivo.					
30590183	5	120	dep	,	839:839	arg1	higher					857:862	higher	857:862	higher	857:862	The compressive modulus is diminished, but three times higher than in the clinically used collagen type I/III scaffold that served as control.					
30590183	2	121	theme	AuriScaff	347:355	arg1	scaffold					414:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	an enzymatically perforated bovine auricular cartilage scaffold	359:421	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30590183	2	121	theme	AuriScaff	347:355	arg1	"					356:356	"AuriScaff"	346:356	"AuriScaff"	346:356	In this study we demonstrate the use of "AuriScaff", an enzymatically perforated bovine auricular cartilage scaffold, as a novel biomaterial for repopulation with regenerative cells and for the formation of high-quality hyaline cartilage.					
30150553	7	0	theme	p	1470:1470	arg1	<					1472:1472	p < 0.05	1470:1477	p < 0.05	1470:1477	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	4	1	theme	%	1061:1061	arg1	MGO-CTS					1063:1069	0.5% MGO-CTS	1058:1069	0.5% MGO-CTS	1058:1069	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	5	2	theme	liver	1133:1137	arg1	weights					1118:1124	the relative weights	1105:1124	the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT),	1105:1287	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	7	3	dep	feed	1619:1622	arg1	T5					1632:1633	T5	1632:1633	T5	1632:1633	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	7	3	dep	feed	1619:1622	arg1	T4					1625:1626	T4	1625:1626	T4	1625:1626	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	7	4	theme	significant	1457:1467	arg1	reduction					1480:1488	A significant (p < 0.05) reduction	1455:1488	A significant (p < 0.05) reduction in the total AFs	1455:1505	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	5	5	theme	kidney	1143:1148	arg1	weights					1118:1124	the relative weights	1105:1124	the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT),	1105:1287	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	1	6	theme	increasing	267:276	arg1	attention					278:286	increasing attention	267:286	increasing attention	267:286	The exploration of feed mycotoxin adsorbents to mitigate the adverse effects of mycotoxins on animals has received increasing attention over the last decade.					
30150553	5	7	theme	relative	1109:1116	arg1	weights					1118:1124	the relative weights	1105:1124	the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT),	1105:1287	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	1	8	theme	feed	171:174	arg1	adsorbents					186:195	feed mycotoxin adsorbents	171:195	feed mycotoxin adsorbents	171:195	The exploration of feed mycotoxin adsorbents to mitigate the adverse effects of mycotoxins on animals has received increasing attention over the last decade.					
30150553	8	9	theme	broiler	1891:1897	arg1	chickens					1899:1906	broiler chickens	1891:1906	broiler chickens	1891:1906	The results of the study indicated that a higher concentration of MGO-CTS (0.50%) was effective in improving the overall performance of broiler chickens by preventing the adverse effects associated with aflatoxicosis.					
30150553	4	10	theme	MGO-CTS	880:886	arg1	%					874:874	0.25 and 0.50%	861:874	0.25 and 0.50%	861:874	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	4	10	theme	MGO-CTS	880:886	arg1	levels					853:858	The two inclusion levels	835:858	The two inclusion levels (0.25 and 0.50%) of MGO-CTS	835:886	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	8	11	theme	overall	1868:1874	arg1	performance					1876:1886	the overall performance	1864:1886	the overall performance of broiler chickens	1864:1906	The results of the study indicated that a higher concentration of MGO-CTS (0.50%) was effective in improving the overall performance of broiler chickens by preventing the adverse effects associated with aflatoxicosis.					
30150553	1	12	theme	adsorbents	186:195	arg1	exploration					156:166	The exploration	152:166	The exploration of feed mycotoxin adsorbents to mitigate the adverse effects of mycotoxins on animals	152:252	The exploration of feed mycotoxin adsorbents to mitigate the adverse effects of mycotoxins on animals has received increasing attention over the last decade.					
30150553	5	13	from	alterations	1176:1186	arg1	SGPT/alanine					1269:1280	SGPT/alanine	1269:1280	SGPT/alanine (ALT)	1269:1286	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	5	13	from	alterations	1176:1186	arg1	parameters					1213:1222	the serum biochemical parameters	1191:1222	the serum biochemical parameters	1191:1222	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	5	13	from	alterations	1176:1186	arg1	albumins					1233:1240	albumins	1233:1240	albumins	1233:1240	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	5	13	from	alterations	1176:1186	arg1	phosphatase					1252:1262	alkaline phosphatase	1243:1262	alkaline phosphatase	1243:1262	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	3	14	theme	AF	821:822	arg1	T6					830:831	T6	830:831	T6	830:831	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	14	theme	AF	821:822	arg1	diet					824:827	AF diet	821:827	AF diet (T6)	821:832	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	0	15	theme	Broilers	105:112	arg1	Biochemistry					70:81	Serum Biochemistry	64:81	Serum Biochemistry	64:81	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	0	15	theme	Broilers	105:112	arg1	Weights					94:100	Organ Weights	88:100	Organ Weights	88:100	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	0	15	theme	Broilers	105:112	arg1	Performance					51:61	Growth Performance	44:61	Growth Performance	44:61	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	3	16	theme	1-day-old	529:537	arg1	chicks					539:544	three hundred 1-day-old chicks	515:544	three hundred 1-day-old chicks	515:544	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	6	17	theme	AF	1352:1353	arg1	diets					1355:1359	AF diets	1352:1359	AF diets	1352:1359	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	1	18	theme	adverse	213:219	arg1	effects					221:227	the adverse effects	209:227	the adverse effects of mycotoxins on animals	209:252	The exploration of feed mycotoxin adsorbents to mitigate the adverse effects of mycotoxins on animals has received increasing attention over the last decade.					
30150553	7	19	from	%	1575:1575	arg1	combination					1599:1609	combination	1599:1609	combination with AF feed (T4 and T5)	1599:1634	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	5	20	theme	age	1303:1305	arg1	days					1295:1298	35 days	1292:1298	35 days of age	1292:1305	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	3	21	theme	basal	691:695	arg1	diet					697:700	basal diet	691:700	basal diet	691:700	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	21	theme	basal	691:695	arg1	T2					719:720	T2	719:720	T2	719:720	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	22	theme	%	741:741	arg1	MGO-CTS					743:749	0.50% MGO-CTS	737:749	0.50% MGO-CTS	737:749	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	6	23	theme	0.5	1334:1336	arg1	%					1337:1337	%	1337:1337	%	1337:1337	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	7	24	theme	%	1669:1669	arg1	decreases					1642:1650	decreases	1642:1650	decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6)	1642:1752	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	4	25	dep	significantly	888:900	arg1	<					905:905	p < 0.05	903:910	p < 0.05	903:910	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	5	26	dep	liver	1133:1137	arg1	the					1129:1131	the	1129:1131	the	1129:1131	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	3	27	theme	%	708:708	arg1	MGO-CTS					710:716	0.25% MGO-CTS	704:716	0.25% MGO-CTS	704:716	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	28	theme	treatment	589:597	arg1	groups					599:604	six dietary treatment groups	577:604	six dietary treatment groups	577:604	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	6	29	theme	AF-induced	1401:1410	arg1	lesions					1418:1424	AF-induced organ lesions	1401:1424	AF-induced organ lesions	1401:1424	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	3	30	theme	basal	724:728	arg1	diet					730:733	basal diet	724:733	basal diet	724:733	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	0	31	from	Effects	0:6	arg1	Biochemistry					70:81	Serum Biochemistry	64:81	Serum Biochemistry	64:81	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	0	31	from	Effects	0:6	arg1	Weights					94:100	Organ Weights	88:100	Organ Weights	88:100	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	0	31	from	Effects	0:6	arg1	Performance					51:61	Growth Performance	44:61	Growth Performance	44:61	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	5	32	theme	serum	1195:1199	arg1	SGPT/alanine					1269:1280	SGPT/alanine	1269:1280	SGPT/alanine (ALT)	1269:1286	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	5	32	theme	serum	1195:1199	arg1	parameters					1213:1222	the serum biochemical parameters	1191:1222	the serum biochemical parameters	1191:1222	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	5	32	theme	serum	1195:1199	arg1	albumins					1233:1240	albumins	1233:1240	albumins	1233:1240	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	5	32	theme	serum	1195:1199	arg1	phosphatase					1252:1262	alkaline phosphatase	1243:1262	alkaline phosphatase	1243:1262	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	2	33	theme	oxide	401:405	arg1	adsorbents					431:440	nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents	368:440	nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents	368:440	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	3	34	theme	AF	757:758	arg1	diet					760:763	AF diet	757:763	AF diet	757:763	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	5	35	theme	significant	1164:1174	arg1	alterations					1176:1186	significant alterations	1164:1186	significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT)	1164:1286	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	2	36	theme	magnetic	383:390	arg1	oxide					401:405	magnetic graphene oxide	383:405	nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents	368:440	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	2	36	theme	magnetic	383:390	arg1	MGO-CTS					422:428	MGO-CTS	422:428	MGO-CTS	422:428	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	2	37	theme	adsorbents	431:440	arg1	efficacy					356:363	the efficacy	352:363	the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents	352:440	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	4	38	theme	feed	950:953	arg1	ratios					966:971	feed conversion ratios	950:971	feed conversion ratios	950:971	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	0	39	theme	Nano-Composite	11:24	arg1	Adsorbents					26:35	Nano-Composite Adsorbents	11:35	Nano-Composite Adsorbents	11:35	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	8	40	theme	MGO-CTS	1821:1827	arg1	effective					1841:1849	effective	1841:1849	effective	1841:1849	The results of the study indicated that a higher concentration of MGO-CTS (0.50%) was effective in improving the overall performance of broiler chickens by preventing the adverse effects associated with aflatoxicosis.					
30150553	8	40	theme	MGO-CTS	1821:1827	arg1	concentration					1804:1816	a higher concentration	1795:1816	a higher concentration of MGO-CTS (0.50%)	1795:1835	The results of the study indicated that a higher concentration of MGO-CTS (0.50%) was effective in improving the overall performance of broiler chickens by preventing the adverse effects associated with aflatoxicosis.					
30150553	3	41	theme	%	771:771	arg1	MGO-CTS					773:779	0.25% MGO-CTS	767:779	0.25% MGO-CTS (T4)	767:784	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	41	theme	%	771:771	arg1	T4					782:783	T4	782:783	T4	782:783	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	7	42	from	reduction	1480:1488	arg1	AFs					1503:1505	the total AFs	1493:1505	the total AFs	1493:1505	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	2	43	theme	ng/g	477:480	arg1	AF					499:500	AF	499:500	AF	499:500	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	2	43	theme	ng/g	477:480	arg1	aflatoxin					488:496	~20 ng/g (ppb) aflatoxin	473:496	~20 ng/g (ppb) aflatoxin (AF)	473:501	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	6	44	theme	profiles	1445:1452	arg1	recovery					1374:1381	apparent recovery	1365:1381	apparent recovery	1365:1381	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	6	44	theme	profiles	1445:1452	arg1	restoration					1386:1396	restoration	1386:1396	restoration	1386:1396	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	5	45	theme	alkaline	1243:1250	arg1	phosphatase					1252:1262	alkaline phosphatase	1243:1262	alkaline phosphatase	1243:1262	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	3	46	theme	basal	619:623	arg1	T3					752:753	T3	752:753	T3	752:753	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	46	theme	basal	619:623	arg1	broilers					631:638	broilers	631:638	broilers fed a diet with neither AF nor MGO-CTS added	631:683	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	46	theme	basal	619:623	arg1	diet					625:628	basal diet	619:628	basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1)	619:688	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	47	theme	AF	787:788	arg1	diet					790:793	AF diet	787:793	AF diet	787:793	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	6	48	theme	aberrant	1430:1437	arg1	profiles					1445:1452	aberrant serum profiles	1430:1452	aberrant serum profiles	1430:1452	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	0	49	theme	Growth	44:49	arg1	Performance					51:61	Growth Performance	44:61	Growth Performance	44:61	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	4	50	theme	inclusion	843:851	arg1	%					874:874	0.25 and 0.50%	861:874	0.25 and 0.50%	861:874	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	4	50	theme	inclusion	843:851	arg1	levels					853:858	The two inclusion levels	835:858	The two inclusion levels (0.25 and 0.50%) of MGO-CTS	835:886	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	6	51	theme	lesions	1418:1424	arg1	recovery					1374:1381	apparent recovery	1365:1381	apparent recovery	1365:1381	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	6	51	theme	lesions	1418:1424	arg1	restoration					1386:1396	restoration	1386:1396	restoration	1386:1396	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	4	52	theme	chicks	991:996	arg1	performances					933:944	growth performances	926:944	growth performances	926:944	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	4	52	theme	chicks	991:996	arg1	ratios					966:971	feed conversion ratios	950:971	feed conversion ratios	950:971	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	3	53	theme	%	801:801	arg1	T5					812:813	T5	812:813	T5	812:813	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	53	theme	%	801:801	arg1	MGO-CTS					803:809	0.50% MGO-CTS	797:809	0.50% MGO-CTS (T5)	797:814	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	7	54	theme	AF-contaminated	1728:1742	arg1	T6					1750:1751	T6	1750:1751	T6	1750:1751	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	7	54	theme	AF-contaminated	1728:1742	arg1	diet					1744:1747	an AF-contaminated diet	1725:1747	an AF-contaminated diet (T6)	1725:1752	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	T6					830:831	T6	830:831	T6	830:831	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	T2					719:720	T2	719:720	T2	719:720	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	diet					697:700	basal diet	691:700	basal diet	691:700	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	MGO-CTS					743:749	0.50% MGO-CTS	737:749	0.50% MGO-CTS	737:749	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	diet					760:763	AF diet	757:763	AF diet	757:763	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	diet					824:827	AF diet	821:827	AF diet (T6)	821:832	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	diet					625:628	basal diet	619:628	basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1)	619:688	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	diet					730:733	basal diet	724:733	basal diet	724:733	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	T3					752:753	T3	752:753	T3	752:753	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	diet					790:793	AF diet	787:793	AF diet	787:793	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	55	dep	distributed	560:570	arg1	broilers					631:638	broilers	631:638	broilers fed a diet with neither AF nor MGO-CTS added	631:683	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	1	56	theme	mycotoxins	232:241	arg1	effects					221:227	the adverse effects	209:227	the adverse effects of mycotoxins on animals	209:252	The exploration of feed mycotoxin adsorbents to mitigate the adverse effects of mycotoxins on animals has received increasing attention over the last decade.					
30150553	7	57	from	%	1584:1584	arg1	combination					1599:1609	combination	1599:1609	combination with AF feed (T4 and T5)	1599:1634	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	5	58	theme	AF	1076:1077	arg1	intake					1079:1084	The AF intake	1072:1084	The AF intake	1072:1084	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	3	59	with	diet	646:649	arg1	MGO-CTS					671:677	MGO-CTS	671:677	MGO-CTS added	671:683	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	3	59	with	diet	646:649	arg1	AF					664:665	AF	664:665	AF	664:665	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	2	60	with	oxide	401:405	arg1	chitosan					412:419	chitosan	412:419	chitosan	412:419	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	4	61	theme	0.5	1058:1060	arg1	%					1061:1061	%	1061:1061	%	1061:1061	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	3	62	dep	broilers	631:638	arg1	T1					686:687	T1	686:687	T1	686:687	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	2	63	theme	present	314:320	arg1	study					322:326	The present study	310:326	The present study	310:326	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	7	64	located	observed	1511:1518	arg2	reduction					1480:1488	A significant (p < 0.05) reduction	1455:1488	A significant (p < 0.05) reduction in the total AFs	1455:1505	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	7	64	located	observed	1511:1518	arg1	tracts					1544:1549	the gastrointestinal tracts	1523:1549	the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6)	1523:1752	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	8	65	theme	chickens	1899:1906	arg1	performance					1876:1886	the overall performance	1864:1886	the overall performance of broiler chickens	1864:1906	The results of the study indicated that a higher concentration of MGO-CTS (0.50%) was effective in improving the overall performance of broiler chickens by preventing the adverse effects associated with aflatoxicosis.					
30150553	3	66	theme	chicks	539:544	arg1	total					506:510	A total	504:510	A total of three hundred 1-day-old chicks	504:544	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	1	67	theme	mycotoxin	176:184	arg1	adsorbents					186:195	feed mycotoxin adsorbents	171:195	feed mycotoxin adsorbents	171:195	The exploration of feed mycotoxin adsorbents to mitigate the adverse effects of mycotoxins on animals has received increasing attention over the last decade.					
30150553	7	68	theme	AF	1616:1617	arg1	feed					1619:1622	AF feed	1616:1622	AF feed (T4 and T5)	1616:1634	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	8	69	theme	adverse	1926:1932	arg1	effects					1934:1940	the adverse effects	1922:1940	the adverse effects associated with aflatoxicosis	1922:1970	The results of the study indicated that a higher concentration of MGO-CTS (0.50%) was effective in improving the overall performance of broiler chickens by preventing the adverse effects associated with aflatoxicosis.					
30150553	0	70	theme	Aflatoxin-Contaminated	123:144	arg1	Feed					146:149	Aflatoxin-Contaminated Feed	123:149	Aflatoxin-Contaminated Feed	123:149	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	7	71	theme	total	1497:1501	arg1	AFs					1503:1505	the total AFs	1493:1505	the total AFs	1493:1505	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	7	72	theme	adsorbent	1586:1594	arg1	%					1584:1584	0.50% adsorbent	1580:1594	0.50% adsorbent in combination with AF feed (T4 and T5)	1580:1634	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	6	73	theme	%	1337:1337	arg1	MGO-CTS					1339:1345	0.5% MGO-CTS	1334:1345	0.5% MGO-CTS	1334:1345	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	8	74	theme	study	1774:1778	arg1	results					1759:1765	The results	1755:1765	The results of the study	1755:1778	The results of the study indicated that a higher concentration of MGO-CTS (0.50%) was effective in improving the overall performance of broiler chickens by preventing the adverse effects associated with aflatoxicosis.					
30150553	4	75	theme	growth	926:931	arg1	performances					933:944	growth performances	926:944	growth performances	926:944	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	1	76	from	effects	221:227	arg1	animals					246:252	animals	246:252	animals	246:252	The exploration of feed mycotoxin adsorbents to mitigate the adverse effects of mycotoxins on animals has received increasing attention over the last decade.					
30150553	4	77	theme	p	903:903	arg1	<					905:905	p < 0.05	903:910	p < 0.05	903:910	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	0	78	theme	Serum	64:68	arg1	Biochemistry					70:81	Serum Biochemistry	64:81	Serum Biochemistry	64:81	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	3	79	theme	dietary	581:587	arg1	groups					599:604	six dietary treatment groups	577:604	six dietary treatment groups	577:604	A total of three hundred 1-day-old chicks were randomly distributed into six dietary treatment groups, as follows: basal diet (broilers fed a diet with neither AF nor MGO-CTS added, T1), basal diet + 0.25% MGO-CTS (T2), basal diet + 0.50% MGO-CTS (T3), AF diet + 0.25% MGO-CTS (T4), AF diet + 0.50% MGO-CTS (T5), and AF diet (T6).					
30150553	6	80	theme	organ	1412:1416	arg1	lesions					1418:1424	AF-induced organ lesions	1401:1424	AF-induced organ lesions	1401:1424	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	8	81	theme	higher	1797:1802	arg1	effective					1841:1849	effective	1841:1849	effective	1841:1849	The results of the study indicated that a higher concentration of MGO-CTS (0.50%) was effective in improving the overall performance of broiler chickens by preventing the adverse effects associated with aflatoxicosis.					
30150553	8	81	theme	higher	1797:1802	arg1	concentration					1804:1816	a higher concentration	1795:1816	a higher concentration of MGO-CTS (0.50%)	1795:1835	The results of the study indicated that a higher concentration of MGO-CTS (0.50%) was effective in improving the overall performance of broiler chickens by preventing the adverse effects associated with aflatoxicosis.					
30150553	7	82	theme	%	1659:1659	arg1	decreases					1642:1650	decreases	1642:1650	decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6)	1642:1752	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	0	83	theme	Organ	88:92	arg1	Weights					94:100	Organ Weights	88:100	Organ Weights	88:100	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	5	84	theme	biochemical	1201:1211	arg1	SGPT/alanine					1269:1280	SGPT/alanine	1269:1280	SGPT/alanine (ALT)	1269:1286	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	5	84	theme	biochemical	1201:1211	arg1	parameters					1213:1222	the serum biochemical parameters	1191:1222	the serum biochemical parameters	1191:1222	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	5	84	theme	biochemical	1201:1211	arg1	albumins					1233:1240	albumins	1233:1240	albumins	1233:1240	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	5	84	theme	biochemical	1201:1211	arg1	phosphatase					1252:1262	alkaline phosphatase	1243:1262	alkaline phosphatase	1243:1262	The AF intake markedly increased the relative weights of the liver and kidney, resulting in significant alterations in the serum biochemical parameters, such as albumins, alkaline phosphatase, and SGPT/alanine (ALT), at 35 days of age.					
30150553	4	85	theme	AF-treated	980:989	arg1	chicks					991:996	the AF-treated chicks	976:996	the AF-treated chicks	976:996	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	7	86	theme	chickens	1558:1565	arg1	tracts					1544:1549	the gastrointestinal tracts	1523:1549	the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6)	1523:1752	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	6	87	theme	apparent	1365:1372	arg1	recovery					1374:1381	apparent recovery	1365:1381	apparent recovery	1365:1381	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	7	88	with	combination	1599:1609	arg1	feed					1619:1622	AF feed	1616:1622	AF feed (T4 and T5)	1616:1634	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	2	89	theme	graphene	392:399	arg1	oxide					401:405	magnetic graphene oxide	383:405	nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents	368:440	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	2	89	theme	graphene	392:399	arg1	MGO-CTS					422:428	MGO-CTS	422:428	MGO-CTS	422:428	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	2	90	dep	feed	450:453	arg1	contaminated					455:466	contaminated	455:466	feed contaminated with ~20 ng/g (ppb) aflatoxin (AF)	450:501	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	4	91	theme	conversion	955:964	arg1	ratios					966:971	feed conversion ratios	950:971	feed conversion ratios	950:971	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	2	92	theme	nano-composite	368:381	arg1	adsorbents					431:440	nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents	368:440	nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents	368:440	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	0	93	theme	Adsorbents	26:35	arg1	Effects					0:6	Effects	0:6	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers	0:112	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	7	94	theme	gastrointestinal	1527:1542	arg1	tracts					1544:1549	the gastrointestinal tracts	1523:1549	the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6)	1523:1752	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
30150553	6	95	contain	had	1361:1363	arg1	chickens					1321:1328	the chickens	1317:1328	the chickens fed 0.5% MGO-CTS with AF diets	1317:1359	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	6	95	contain	had	1361:1363	arg2	restoration					1386:1396	restoration	1386:1396	restoration	1386:1396	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	6	95	contain	had	1361:1363	arg2	recovery					1374:1381	apparent recovery	1365:1381	apparent recovery	1365:1381	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	4	96	theme	age	1014:1016	arg1	days					1006:1009	1⁻35 days	1001:1009	1⁻35 days of age	1001:1016	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	1	97	theme	last	297:300	arg1	decade					302:307	the last decade	293:307	the last decade	293:307	The exploration of feed mycotoxin adsorbents to mitigate the adverse effects of mycotoxins on animals has received increasing attention over the last decade.					
30150553	0	98	dep	Performance	51:61	arg1	the					40:42	the	40:42	the	40:42	Effects of Nano-Composite Adsorbents on the Growth Performance, Serum Biochemistry, and Organ Weights of Broilers Fed with Aflatoxin-Contaminated Feed.					
30150553	4	99	dep	performances	933:944	arg1	the					922:924	the	922:924	the	922:924	The two inclusion levels (0.25 and 0.50%) of MGO-CTS significantly (p < 0.05) improved the growth performances and feed conversion ratios of the AF-treated chicks at 1⁻35 days of age, and the impact was more pronounced for 0.5% MGO-CTS.					
30150553	6	100	theme	serum	1439:1443	arg1	profiles					1445:1452	aberrant serum profiles	1430:1452	aberrant serum profiles	1430:1452	However, the chickens fed 0.5% MGO-CTS with AF diets had apparent recovery or restoration of AF-induced organ lesions and aberrant serum profiles.					
30150553	2	101	dep	aflatoxin	488:496	arg1	ppb					483:485	ppb	483:485	ppb	483:485	The present study was conducted to assess the efficacy of nano-composite magnetic graphene oxide with chitosan (MGO-CTS) adsorbents against feed contaminated with ~20 ng/g (ppb) aflatoxin (AF).					
30150553	7	102	dep	significant	1457:1467	arg1	<					1472:1472	p < 0.05	1470:1477	p < 0.05	1470:1477	A significant (p < 0.05) reduction in the total AFs was observed in the gastrointestinal tracts of the chickens fed 0.25% or 0.50% adsorbent in combination with AF feed (T4 and T5), with decreases of 28.9% and 53.5%, respectively, compared with that in the chickens fed an AF-contaminated diet (T6).					
31732023	7	0	theme	significant	1157:1167	arg1	change					1169:1174	a significant change	1155:1174	a significant change (P < .05)	1155:1184	The texture characteristics of hardness, elasticity and chewiness had a significant change (P < .05).					
31732023	7	0	theme	significant	1157:1167	arg1	P < .05					1177:1183	P < .05	1177:1183	P < .05	1177:1183	The texture characteristics of hardness, elasticity and chewiness had a significant change (P < .05).					
31732023	1	1	theme	ultrasound	171:180	arg1	impact					161:166	The synergistic impact	145:166	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage	145:274	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage was investigated.					
31732023	4	2	from	difference	657:666	arg1	loss					704:707	thawing loss	696:707	thawing loss	696:707	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	4	2	from	difference	657:666	arg1	content					716:722	water content	710:722	water content	710:722	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	4	2	from	difference	657:666	arg1	activity					734:741	water activity	728:741	water activity	728:741	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	4	2	from	difference	657:666	arg1	gain					690:693	the soaking weight gain	671:693	the soaking weight gain	671:693	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	6	3	theme	frozen	953:958	arg1	period					968:973	the frozen storage period	949:973	the frozen storage period	949:973	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	8	4	theme	protein	1257:1263	arg1	content					1265:1271	myofibrillar protein content	1244:1271	myofibrillar protein content	1244:1271	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	5	5	theme	better	841:846	arg1	effect					864:869	better water retention effect	841:869	better water retention effect	841:869	The ultrasound combined with WRA treatment showed better water retention effect.					
31732023	5	6	theme	water	848:852	arg1	effect					864:869	better water retention effect	841:869	better water retention effect	841:869	The ultrasound combined with WRA treatment showed better water retention effect.					
31732023	9	7	theme	crayfish	1492:1499	arg1	quality					1481:1487	the quality	1477:1487	the quality of crayfish	1477:1499	Therefore, WRA-US60W treatment was found effectively improving the quality of crayfish during frozen storage.					
31732023	3	8	from	indices	532:538	arg1	42 days					586:592	0, 7, 14, 21, 28, 35 and 42 days	561:592	0, 7, 14, 21, 28, 35 and 42 days	561:592	The indices of frozen storage in 0, 7, 14, 21, 28, 35 and 42 days were measured.					
31732023	2	9	theme	water-retaining	316:330	arg1	WRA					339:341	WRA	339:341	WRA	339:341	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	2	9	theme	water-retaining	316:330	arg1	agent					332:336	water-retaining agent	316:336	water-retaining agent (WRA)	316:342	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	8	10	theme	activity	1289:1296	arg1	development					1191:1201	The development	1187:1201	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein	1187:1314	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	1	11	from	impact	161:166	arg1	effect					235:240	the cryoprotective effect	216:240	the cryoprotective effect on crayfish during frozen storage	216:274	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage was investigated.					
31732023	4	12	theme	water	710:714	arg1	content					716:722	water content	710:722	water content	710:722	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	4	13	theme	weight	683:688	arg1	gain					690:693	the soaking weight gain	671:693	the soaking weight gain	671:693	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	0	14	theme	storage	126:132	arg1	qualities					134:142	storage qualities	126:142	storage qualities	126:142	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	5	15	theme	retention	854:862	arg1	effect					864:869	better water retention effect	841:869	better water retention effect	841:869	The ultrasound combined with WRA treatment showed better water retention effect.					
31732023	8	16	theme	nitrogen	1226:1233	arg1	content					1235:1241	total volatile base nitrogen content	1206:1241	total volatile base nitrogen content	1206:1241	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	3	17	theme	storage	550:556	arg1	indices					532:538	The indices	528:538	The indices of frozen storage in 0, 7, 14, 21, 28, 35 and 42 days	528:592	The indices of frozen storage in 0, 7, 14, 21, 28, 35 and 42 days were measured.					
31732023	6	18	theme	higher	1024:1029	arg1	values					1031:1036	significantly higher values	1010:1036	significantly higher values	1010:1036	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	1	19	theme	Nano-water	186:195	arg1	agent					207:211	Nano-water retaining agent	186:211	Nano-water retaining agent	186:211	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage was investigated.					
31732023	7	20	theme	chewiness	1141:1149	arg1	characteristics					1097:1111	The texture characteristics	1085:1111	The texture characteristics of hardness, elasticity and chewiness	1085:1149	The texture characteristics of hardness, elasticity and chewiness had a significant change (P < .05).					
31732023	7	21	theme	texture	1089:1095	arg1	characteristics					1097:1111	The texture characteristics	1085:1111	The texture characteristics of hardness, elasticity and chewiness	1085:1149	The texture characteristics of hardness, elasticity and chewiness had a significant change (P < .05).					
31732023	8	22	theme	content	1235:1241	arg1	development					1191:1201	The development	1187:1201	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein	1187:1314	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	7	23	theme	elasticity	1126:1135	arg1	characteristics					1097:1111	The texture characteristics	1085:1111	The texture characteristics of hardness, elasticity and chewiness	1085:1149	The texture characteristics of hardness, elasticity and chewiness had a significant change (P < .05).					
31732023	1	24	theme	retaining	197:205	arg1	agent					207:211	Nano-water retaining agent	186:211	Nano-water retaining agent	186:211	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage was investigated.					
31732023	3	25	theme	frozen	543:548	arg1	storage					550:556	frozen storage	543:556	frozen storage	543:556	The indices of frozen storage in 0, 7, 14, 21, 28, 35 and 42 days were measured.					
31732023	8	26	theme	WRA-US60W	1346:1354	arg1	treatment					1356:1364	WRA-US60W treatment	1346:1364	WRA-US60W treatment	1346:1364	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	4	27	theme	thawing	696:702	arg1	loss					704:707	thawing loss	696:707	thawing loss	696:707	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	1	28	theme	agent	207:211	arg1	impact					161:166	The synergistic impact	145:166	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage	145:274	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage was investigated.					
31732023	0	29	theme	Ultrasound	0:9	arg1	treatment					11:19	Ultrasound treatment	0:19	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.	0:143	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	8	30	theme	protein	1308:1314	arg1	content					1265:1271	myofibrillar protein content	1244:1271	myofibrillar protein content	1244:1271	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	8	30	theme	protein	1308:1314	arg1	activity					1289:1296	Ca2+-ATPase activity	1277:1296	Ca2+-ATPase activity	1277:1296	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	8	30	theme	protein	1308:1314	arg1	content					1235:1241	total volatile base nitrogen content	1206:1241	total volatile base nitrogen content	1206:1241	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	5	31	theme	WRA	820:822	arg1	treatment					824:832	WRA treatment	820:832	WRA treatment	820:832	The ultrasound combined with WRA treatment showed better water retention effect.					
31732023	0	32	theme	frozen	24:29	arg1	crayfish					31:38	frozen crayfish	24:38	frozen crayfish	24:38	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	6	33	theme	gel	915:917	arg1	capacity					890:897	The water holding capacity	872:897	The water holding capacity of heat induced gel	872:917	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	8	34	theme	total	1206:1210	arg1	content					1235:1241	total volatile base nitrogen content	1206:1241	total volatile base nitrogen content	1206:1241	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	7	35	theme	hardness	1116:1123	arg1	characteristics					1097:1111	The texture characteristics	1085:1111	The texture characteristics of hardness, elasticity and chewiness	1085:1149	The texture characteristics of hardness, elasticity and chewiness had a significant change (P < .05).					
31732023	6	36	theme	induced	907:913	arg1	gel					915:917	heat induced gel	902:917	heat induced gel	902:917	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	4	37	theme	water	728:732	arg1	activity					734:741	water activity	728:741	water activity	728:741	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	0	38	from	Influence	92:100	arg1	cryopreservation					105:120	cryopreservation	105:120	cryopreservation	105:120	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	0	38	from	Influence	92:100	arg1	qualities					134:142	storage qualities	126:142	storage qualities	126:142	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	1	39	theme	cryoprotective	220:233	arg1	effect					235:240	the cryoprotective effect	216:240	the cryoprotective effect on crayfish during frozen storage	216:274	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage was investigated.					
31732023	2	40	theme	frozen	502:507	arg1	storage					509:515	frozen storage	502:515	frozen storage	502:515	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	2	40	theme	frozen	502:507	arg1	samples					298:304	The samples	294:304	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W)	294:495	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	6	41	theme	heat	902:905	arg1	gel					915:917	heat induced gel	902:917	heat induced gel	902:917	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	6	42	theme	WRA-US60W	984:992	arg1	group					994:998	the WRA-US60W group	980:998	the WRA-US60W group	980:998	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	4	43	theme	WRA	769:771	arg1	P < .05					781:787	P < .05	781:787	P < .05	781:787	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	4	43	theme	WRA	769:771	arg1	groups					773:778	WRA groups	769:778	WRA groups (P < .05)	769:788	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	2	44	theme	80 W	434:437	arg1	treatment					439:447	ultrasonic 80 W treatment	423:447	ultrasonic 80 W treatment	423:447	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	0	45	theme	crayfish	31:38	arg1	treatment					11:19	Ultrasound treatment	0:19	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.	0:143	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	8	46	theme	base	1221:1224	arg1	content					1235:1241	total volatile base nitrogen content	1206:1241	total volatile base nitrogen content	1206:1241	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	2	47	theme	water-retaining	463:477	arg1	agent					479:483	water-retaining agent	463:483	water-retaining agent (WRA-US80W)	463:495	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	2	47	theme	water-retaining	463:477	arg1	WRA-US80W					486:494	WRA-US80W	486:494	WRA-US80W	486:494	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	1	48	from	effect	235:240	arg1	crayfish					245:252	crayfish	245:252	crayfish	245:252	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage was investigated.					
31732023	2	49	theme	ultrasonic	423:432	arg1	treatment					439:447	ultrasonic 80 W treatment	423:447	ultrasonic 80 W treatment	423:447	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	0	50	theme	Nano-composite	54:67	arg1	Influence					92:100	chitosan Nano-composite water-retaining agent: Influence	45:100	chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities	45:142	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	2	51	from	-18 °C	520:525	arg1	storage					509:515	frozen storage	502:515	frozen storage	502:515	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	2	51	from	-18 °C	520:525	arg1	samples					298:304	The samples	294:304	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W)	294:495	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	6	52	theme	treatment	1057:1065	arg1	P < .05					1075:1081	P < .05	1075:1081	P < .05	1075:1081	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	6	52	theme	treatment	1057:1065	arg1	groups					1067:1072	other treatment groups	1051:1072	other treatment groups (P < .05)	1051:1082	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	4	53	theme	soaking	675:681	arg1	gain					690:693	the soaking weight gain	671:693	the soaking weight gain	671:693	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	0	54	theme	chitosan	45:52	arg1	Influence					92:100	chitosan Nano-composite water-retaining agent: Influence	45:100	chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities	45:142	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	6	55	theme	holding	882:888	arg1	capacity					890:897	The water holding capacity	872:897	The water holding capacity of heat induced gel	872:917	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	0	56	with	treatment	11:19	arg1	Influence					92:100	chitosan Nano-composite water-retaining agent: Influence	45:100	chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities	45:142	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	2	57	theme	60 W	356:359	arg1	treatment					361:369	ultrasonic 60 W treatment	345:369	ultrasonic 60 W treatment	345:369	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	8	58	theme	structure	1403:1411	arg1	integrity					1383:1391	the integrity	1379:1391	the integrity of tissue structure	1379:1411	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	6	59	theme	water	876:880	arg1	capacity					890:897	The water holding capacity	872:897	The water holding capacity of heat induced gel	872:917	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	7	60	contain	had	1151:1153	arg2	P < .05					1177:1183	P < .05	1177:1183	P < .05	1177:1183	The texture characteristics of hardness, elasticity and chewiness had a significant change (P < .05).					
31732023	7	60	contain	had	1151:1153	arg2	change					1169:1174	a significant change	1155:1174	a significant change (P < .05)	1155:1184	The texture characteristics of hardness, elasticity and chewiness had a significant change (P < .05).					
31732023	7	60	contain	had	1151:1153	arg1	characteristics					1097:1111	The texture characteristics	1085:1111	The texture characteristics of hardness, elasticity and chewiness	1085:1149	The texture characteristics of hardness, elasticity and chewiness had a significant change (P < .05).					
31732023	2	61	theme	ultrasonic	345:354	arg1	treatment					361:369	ultrasonic 60 W treatment	345:369	ultrasonic 60 W treatment	345:369	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	8	62	theme	volatile	1212:1219	arg1	content					1235:1241	total volatile base nitrogen content	1206:1241	total volatile base nitrogen content	1206:1241	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	8	63	theme	muscle	1301:1306	arg1	protein					1308:1314	muscle protein	1301:1314	muscle protein	1301:1314	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	8	64	theme	content	1265:1271	arg1	development					1191:1201	The development	1187:1201	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein	1187:1314	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	1	65	theme	synergistic	149:159	arg1	impact					161:166	The synergistic impact	145:166	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage	145:274	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage was investigated.					
31732023	8	66	theme	Ca2+-ATPase	1277:1287	arg1	activity					1289:1296	Ca2+-ATPase activity	1277:1296	Ca2+-ATPase activity	1277:1296	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	8	67	theme	myofibrillar	1244:1255	arg1	content					1265:1271	myofibrillar protein content	1244:1271	myofibrillar protein content	1244:1271	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	0	68	theme	agent	85:89	arg1	Influence					92:100	chitosan Nano-composite water-retaining agent: Influence	45:100	chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities	45:142	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	6	69	theme	other	1051:1055	arg1	P < .05					1075:1081	P < .05	1075:1081	P < .05	1075:1081	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	6	69	theme	other	1051:1055	arg1	groups					1067:1072	other treatment groups	1051:1072	other treatment groups (P < .05)	1051:1082	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31732023	8	70	theme	tissue	1396:1401	arg1	structure					1403:1411	tissue structure	1396:1411	tissue structure	1396:1411	The development of total volatile base nitrogen content, myofibrillar protein content and Ca2+-ATPase activity of muscle protein were significantly delayed by WRA-US60W treatment, maintaining the integrity of tissue structure.					
31732023	9	71	theme	WRA-US60W	1425:1433	arg1	treatment					1435:1443	WRA-US60W treatment	1425:1443	WRA-US60W treatment	1425:1443	Therefore, WRA-US60W treatment was found effectively improving the quality of crayfish during frozen storage.					
31732023	4	72	theme	significant	645:655	arg1	difference					657:666	a significant difference	643:666	a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05)	643:788	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	1	73	theme	frozen	261:266	arg1	storage					268:274	frozen storage	261:274	frozen storage	261:274	The synergistic impact of ultrasound and Nano-water retaining agent on the cryoprotective effect on crayfish during frozen storage was investigated.					
31732023	0	74	theme	water-retaining	69:83	arg1	Influence					92:100	chitosan Nano-composite water-retaining agent: Influence	45:100	chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities	45:142	Ultrasound treatment of frozen crayfish with chitosan Nano-composite water-retaining agent: Influence on cryopreservation and storage qualities.					
31732023	4	75	theme	control	751:757	arg1	group					759:763	control group	751:763	control group	751:763	The results showed that there was a significant difference in the soaking weight gain, thawing loss, water content and water activity between control group and WRA groups (P < .05).					
31732023	2	76	theme	water-retaining	385:399	arg1	WRA-US60W					408:416	WRA-US60W	408:416	WRA-US60W	408:416	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	2	76	theme	water-retaining	385:399	arg1	agent					401:405	water-retaining agent	385:405	water-retaining agent (WRA-US60W)	385:417	The samples soaked in water-retaining agent (WRA), ultrasonic 60 W treatment combined with water-retaining agent (WRA-US60W) and ultrasonic 80 W treatment combined with water-retaining agent (WRA-US80W) were frozen storage at -18 °C.					
31732023	6	77	theme	storage	960:966	arg1	period					968:973	the frozen storage period	949:973	the frozen storage period	949:973	The water holding capacity of heat induced gel decreased continuously during the frozen storage period, and the WRA-US60W group exhibited significantly higher values than that of other treatment groups (P < .05).					
31217009	1	0	theme	brilliant	166:174	arg1	ability					200:206	its brilliant photothermal conversion ability	162:206	its brilliant photothermal conversion ability	162:206	BACKGROUND Molybdenum disulfide (MoS2) has been widely explored for biomedical applications due to its brilliant photothermal conversion ability.					
31217009	6	1	theme	cancer	1215:1220	arg1	cells					1255:1259	drug resistant cells	1240:1259	especially drug resistant cells (A549 and H1975)	1229:1276	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	1	theme	cancer	1215:1220	arg1	cells					1222:1226	CD44-positive lung cancer cells	1196:1226	CD44-positive lung cancer cells	1196:1226	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	1	2	theme	Molybdenum	74:83	arg1	MoS2					96:99	MoS2	96:99	MoS2	96:99	BACKGROUND Molybdenum disulfide (MoS2) has been widely explored for biomedical applications due to its brilliant photothermal conversion ability.					
31217009	1	2	theme	Molybdenum	74:83	arg1	disulfide					85:93	BACKGROUND Molybdenum disulfide	63:93	BACKGROUND Molybdenum disulfide (MoS2)	63:100	BACKGROUND Molybdenum disulfide (MoS2) has been widely explored for biomedical applications due to its brilliant photothermal conversion ability.					
31217009	6	3	theme	resistant	1245:1253	arg1	A549					1262:1265	A549	1262:1265	A549	1262:1265	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	3	theme	resistant	1245:1253	arg1	cells					1255:1259	drug resistant cells	1240:1259	especially drug resistant cells (A549 and H1975)	1229:1276	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	3	theme	resistant	1245:1253	arg1	cells					1222:1226	CD44-positive lung cancer cells	1196:1226	CD44-positive lung cancer cells	1196:1226	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	3	theme	resistant	1245:1253	arg1	H1975					1271:1275	H1975	1271:1275	H1975	1271:1275	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	4	4	theme	physiological	781:793	arg1	solutions					795:803	physiological solutions	781:803	physiological solutions	781:803	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	3	5	theme	photothermal	579:590	arg1	therapy					592:598	photothermal therapy	579:598	photothermal therapy	579:598	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	5	6	theme	laser	940:944	arg1	irradiation					952:962	near infrared laser (NIR) irradiation	926:962	near infrared laser (NIR) irradiation	926:962	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	5	7	used	used	1000:1003	arg2	composites					982:991	the composites	978:991	the composites	978:991	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	5	7	used	used	1000:1003	arg2	systems					1019:1025	responsive systems	1008:1025	responsive systems	1008:1025	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	7	8	theme	photothermal	1417:1428	arg1	therapy					1430:1436	photothermal therapy	1417:1436	photothermal therapy alone	1417:1442	In vivo tumor ablation studies prove a better synergistic therapeutic effect of the joint treatment, compared with either chemotherapy or photothermal therapy alone.					
31217009	7	9	theme	therapeutic	1337:1347	arg1	effect					1349:1354	a better synergistic therapeutic effect	1316:1354	a better synergistic therapeutic effect of the joint treatment	1316:1377	In vivo tumor ablation studies prove a better synergistic therapeutic effect of the joint treatment, compared with either chemotherapy or photothermal therapy alone.					
31217009	1	10	theme	photothermal	176:187	arg1	ability					200:206	its brilliant photothermal conversion ability	162:206	its brilliant photothermal conversion ability	162:206	BACKGROUND Molybdenum disulfide (MoS2) has been widely explored for biomedical applications due to its brilliant photothermal conversion ability.					
31217009	8	11	theme	targeted	1545:1552	arg1	delivery					1559:1566	targeted drug delivery	1545:1566	targeted drug delivery	1545:1566	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	8	12	dep	CONCLUSION	1445:1454	arg1	system					1534:1539	a potent system	1525:1539	a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy	1525:1616	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	8	12	dep	CONCLUSION	1445:1454	arg1	nanoplatform					1480:1491	The functionalized MoS2 nanoplatform	1456:1491	The functionalized MoS2 nanoplatform developed in this work	1456:1514	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	3	13	theme	chemotherapy	562:573	arg1	combination					547:557	the combination	543:557	the combination of chemotherapy and photothermal therapy	543:598	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	1	14	theme	conversion	189:198	arg1	ability					200:206	its brilliant photothermal conversion ability	162:206	its brilliant photothermal conversion ability	162:206	BACKGROUND Molybdenum disulfide (MoS2) has been widely explored for biomedical applications due to its brilliant photothermal conversion ability.					
31217009	3	15	theme	MoS2	382:385	arg1	nanocarrier					456:466	a tumor-targeting chemotherapeutic nanocarrier	421:466	a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery	421:527	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	3	15	theme	MoS2	382:385	arg1	nanosheets					387:396	these functionalized MoS2 nanosheets	361:396	these functionalized MoS2 nanosheets	361:396	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	7	16	dep	In	1279:1280	arg1	vivo					1282:1285	vivo	1282:1285	vivo	1282:1285	In vivo tumor ablation studies prove a better synergistic therapeutic effect of the joint treatment, compared with either chemotherapy or photothermal therapy alone.					
31217009	4	17	theme	high	763:766	arg1	stability					768:776	high stability	763:776	great biocompatibility as well as high stability	729:776	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	8	18	theme	potent	1527:1532	arg1	system					1534:1539	a potent system	1525:1539	a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy	1525:1616	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	8	18	theme	potent	1527:1532	arg1	nanoplatform					1480:1491	The functionalized MoS2 nanoplatform	1456:1491	The functionalized MoS2 nanoplatform developed in this work	1456:1514	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	0	19	theme	Functionalized	0:13	arg1	MoS2-erlotinib					15:28	Functionalized MoS2-erlotinib	0:28	Functionalized MoS2-erlotinib	0:28	Functionalized MoS2-erlotinib produces hyperthermia under NIR.					
31217009	5	20	theme	controllable	1044:1055	arg1	release					1036:1042	Er release	1033:1042	Er release controllable through NIR irradiation	1033:1079	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	5	21	theme	NIR	947:949	arg1	irradiation					952:962	near infrared laser (NIR) irradiation	926:962	near infrared laser (NIR) irradiation	926:962	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	3	22	theme	tumor-targeting	423:437	arg1	nanocarrier					456:466	a tumor-targeting chemotherapeutic nanocarrier	421:466	a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery	421:527	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	3	22	theme	tumor-targeting	423:437	arg1	nanosheets					387:396	these functionalized MoS2 nanosheets	361:396	these functionalized MoS2 nanosheets	361:396	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	6	23	theme	lung	1210:1213	arg1	cells					1255:1259	drug resistant cells	1240:1259	especially drug resistant cells (A549 and H1975)	1229:1276	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	23	theme	lung	1210:1213	arg1	cells					1222:1226	CD44-positive lung cancer cells	1196:1226	CD44-positive lung cancer cells	1196:1226	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	8	24	theme	functionalized	1460:1473	arg1	system					1534:1539	a potent system	1525:1539	a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy	1525:1616	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	8	24	theme	functionalized	1460:1473	arg1	nanoplatform					1480:1491	The functionalized MoS2 nanoplatform	1456:1491	The functionalized MoS2 nanoplatform developed in this work	1456:1514	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	4	25	theme	anti-cancer	845:855	arg1	drug					857:860	the insoluble anti-cancer drug	831:860	the insoluble anti-cancer drug erlotinib (Er)	831:875	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	3	26	theme	therapy	592:598	arg1	combination					547:557	the combination	543:557	the combination of chemotherapy and photothermal therapy	543:598	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	4	27	theme	insoluble	835:843	arg1	drug					857:860	the insoluble anti-cancer drug	831:860	the insoluble anti-cancer drug erlotinib (Er)	831:875	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	4	28	dep	drug	857:860	arg1	Er					873:874	Er	873:874	Er	873:874	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	4	28	dep	drug	857:860	arg1	erlotinib					862:870	erlotinib	862:870	the insoluble anti-cancer drug erlotinib (Er)	831:875	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	3	29	theme	functionalized	367:380	arg1	nanocarrier					456:466	a tumor-targeting chemotherapeutic nanocarrier	421:466	a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery	421:527	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	3	29	theme	functionalized	367:380	arg1	nanosheets					387:396	these functionalized MoS2 nanosheets	361:396	these functionalized MoS2 nanosheets	361:396	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	8	30	theme	synergistic	1572:1582	arg1	therapy					1610:1616	synergistic chemo-photothermal cancer therapy	1572:1616	synergistic chemo-photothermal cancer therapy	1572:1616	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	6	31	theme	MTT	1082:1084	arg1	assays					1086:1091	MTT assays	1082:1091	MTT assays	1082:1091	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	3	32	theme	NIR	487:489	arg1	delivery					520:527	near-infrared (NIR) photothermal-triggered drug delivery	472:527	near-infrared (NIR) photothermal-triggered drug delivery	472:527	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	7	33	theme	tumor	1287:1291	arg1	studies					1302:1308	In vivo tumor ablation studies	1279:1308	In vivo tumor ablation studies	1279:1308	In vivo tumor ablation studies prove a better synergistic therapeutic effect of the joint treatment, compared with either chemotherapy or photothermal therapy alone.					
31217009	5	34	theme	near	926:929	arg1	irradiation					952:962	near infrared laser (NIR) irradiation	926:962	near infrared laser (NIR) irradiation	926:962	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	5	35	theme	NIR	1065:1067	arg1	irradiation					1069:1079	NIR irradiation	1065:1079	NIR irradiation	1065:1079	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	2	36	theme	novel	236:240	arg1	MoS2-SS-HA					291:300	MoS2-SS-HA	291:300	MoS2-SS-HA	291:300	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	2	36	theme	novel	236:240	arg1	system					283:288	a novel multifunctional MoS2-based drug delivery system	234:288	a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA)	234:301	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	6	37	theme	CD44-positive	1196:1208	arg1	cells					1255:1259	drug resistant cells	1240:1259	especially drug resistant cells (A549 and H1975)	1229:1276	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	37	theme	CD44-positive	1196:1208	arg1	cells					1222:1226	CD44-positive lung cancer cells	1196:1226	CD44-positive lung cancer cells	1196:1226	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	3	38	theme	chemotherapeutic	439:454	arg1	nanocarrier					456:466	a tumor-targeting chemotherapeutic nanocarrier	421:466	a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery	421:527	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	3	38	theme	chemotherapeutic	439:454	arg1	nanosheets					387:396	these functionalized MoS2 nanosheets	361:396	these functionalized MoS2 nanosheets	361:396	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	8	39	theme	cancer	1603:1608	arg1	therapy					1610:1616	synergistic chemo-photothermal cancer therapy	1572:1616	synergistic chemo-photothermal cancer therapy	1572:1616	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	8	40	theme	MoS2	1475:1478	arg1	system					1534:1539	a potent system	1525:1539	a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy	1525:1616	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	8	40	theme	MoS2	1475:1478	arg1	nanoplatform					1480:1491	The functionalized MoS2 nanoplatform	1456:1491	The functionalized MoS2 nanoplatform developed in this work	1456:1514	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	5	41	theme	responsive	1008:1017	arg1	composites					982:991	the composites	978:991	the composites	978:991	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	5	41	theme	responsive	1008:1017	arg1	systems					1019:1025	responsive systems	1008:1025	responsive systems	1008:1025	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	7	42	theme	treatment	1369:1377	arg1	effect					1349:1354	a better synergistic therapeutic effect	1316:1354	a better synergistic therapeutic effect of the joint treatment	1316:1377	In vivo tumor ablation studies prove a better synergistic therapeutic effect of the joint treatment, compared with either chemotherapy or photothermal therapy alone.					
31217009	3	43	theme	near-infrared	472:484	arg1	delivery					520:527	near-infrared (NIR) photothermal-triggered drug delivery	472:527	near-infrared (NIR) photothermal-triggered drug delivery	472:527	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	5	44	theme	Er	893:894	arg1	release					882:888	The release	878:888	The release of Er	878:894	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	8	45	theme	drug	1554:1557	arg1	delivery					1559:1566	targeted drug delivery	1545:1566	targeted drug delivery	1545:1566	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
31217009	7	46	theme	joint	1363:1367	arg1	treatment					1369:1377	the joint treatment	1359:1377	the joint treatment	1359:1377	In vivo tumor ablation studies prove a better synergistic therapeutic effect of the joint treatment, compared with either chemotherapy or photothermal therapy alone.					
31217009	6	47	theme	confocal	1097:1104	arg1	imaging					1106:1112	confocal imaging	1097:1112	confocal imaging	1097:1112	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	3	48	theme	drug	515:518	arg1	delivery					520:527	near-infrared (NIR) photothermal-triggered drug delivery	472:527	near-infrared (NIR) photothermal-triggered drug delivery	472:527	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	3	49	theme	MoS2	318:321	arg1	nanosheets					323:332	MoS2 nanosheets	318:332	MoS2 nanosheets	318:332	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	3	50	theme	cancer	620:625	arg1	therapy					627:633	cancer therapy	620:633	cancer therapy	620:633	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	7	51	theme	synergistic	1325:1335	arg1	effect					1349:1354	a better synergistic therapeutic effect	1316:1354	a better synergistic therapeutic effect of the joint treatment	1316:1377	In vivo tumor ablation studies prove a better synergistic therapeutic effect of the joint treatment, compared with either chemotherapy or photothermal therapy alone.					
31217009	4	52	theme	great	729:733	arg1	biocompatibility					735:750	great biocompatibility	729:750	great biocompatibility as well as high stability	729:776	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	1	53	theme	BACKGROUND	63:72	arg1	MoS2					96:99	MoS2	96:99	MoS2	96:99	BACKGROUND Molybdenum disulfide (MoS2) has been widely explored for biomedical applications due to its brilliant photothermal conversion ability.					
31217009	1	53	theme	BACKGROUND	63:72	arg1	disulfide					85:93	BACKGROUND Molybdenum disulfide	63:93	BACKGROUND Molybdenum disulfide (MoS2)	63:100	BACKGROUND Molybdenum disulfide (MoS2) has been widely explored for biomedical applications due to its brilliant photothermal conversion ability.					
31217009	5	54	theme	Er	1033:1034	arg1	release					1036:1042	Er release	1033:1042	Er release controllable through NIR irradiation	1033:1079	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	2	55	theme	delivery	274:281	arg1	MoS2-SS-HA					291:300	MoS2-SS-HA	291:300	MoS2-SS-HA	291:300	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	2	55	theme	delivery	274:281	arg1	system					283:288	a novel multifunctional MoS2-based drug delivery system	234:288	a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA)	234:301	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	6	56	theme	drug	1240:1243	arg1	A549					1262:1265	A549	1262:1265	A549	1262:1265	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	56	theme	drug	1240:1243	arg1	cells					1255:1259	drug resistant cells	1240:1259	especially drug resistant cells (A549 and H1975)	1229:1276	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	56	theme	drug	1240:1243	arg1	cells					1222:1226	CD44-positive lung cancer cells	1196:1226	CD44-positive lung cancer cells	1196:1226	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	56	theme	drug	1240:1243	arg1	H1975					1271:1275	H1975	1271:1275	H1975	1271:1275	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	1	57	theme	biomedical	131:140	arg1	applications					142:153	biomedical applications	131:153	biomedical applications due to its brilliant photothermal conversion ability	131:206	BACKGROUND Molybdenum disulfide (MoS2) has been widely explored for biomedical applications due to its brilliant photothermal conversion ability.					
31217009	6	58	theme	MoS2-based	1138:1147	arg1	nanoplatform					1149:1160	the MoS2-based nanoplatform	1134:1160	the MoS2-based nanoplatform	1134:1160	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	4	59	dep	125 nm	710:715	arg1	ca.					706:708	ca.	706:708	ca.	706:708	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	7	60	theme	In	1279:1280	arg1	studies					1302:1308	In vivo tumor ablation studies	1279:1308	In vivo tumor ablation studies	1279:1308	In vivo tumor ablation studies prove a better synergistic therapeutic effect of the joint treatment, compared with either chemotherapy or photothermal therapy alone.					
31217009	2	61	theme	drug	269:272	arg1	MoS2-SS-HA					291:300	MoS2-SS-HA	291:300	MoS2-SS-HA	291:300	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	2	61	theme	drug	269:272	arg1	system					283:288	a novel multifunctional MoS2-based drug delivery system	234:288	a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA)	234:301	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	3	62	theme	photothermal-triggered	492:513	arg1	delivery					520:527	near-infrared (NIR) photothermal-triggered drug delivery	472:527	near-infrared (NIR) photothermal-triggered drug delivery	472:527	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	5	63	theme	infrared	931:938	arg1	irradiation					952:962	near infrared laser (NIR) irradiation	926:962	near infrared laser (NIR) irradiation	926:962	The release of Er was greatly accelerated under near infrared laser (NIR) irradiation, showing that the composites can be used as responsive systems, with Er release controllable through NIR irradiation.					
31217009	3	64	theme	hyaluronic	339:348	arg1	HA					356:357	HA	356:357	HA	356:357	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	3	64	theme	hyaluronic	339:348	arg1	acid					350:353	hyaluronic acid	339:353	hyaluronic acid (HA)	339:358	By decorating MoS2 nanosheets with hyaluronic acid (HA), these functionalized MoS2 nanosheets have been developed as a tumor-targeting chemotherapeutic nanocarrier for near-infrared (NIR) photothermal-triggered drug delivery, facilitating the combination of chemotherapy and photothermal therapy into one system for cancer therapy.					
31217009	4	65	dep	RESULTS	636:642	arg1	MoS2-SS-HA					664:673	MoS2-SS-HA	664:673	MoS2-SS-HA	664:673	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	4	65	dep	RESULTS	636:642	arg1	nanocomposites					648:661	The nanocomposites	644:661	RESULTS The nanocomposites (MoS2-SS-HA)	636:674	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	2	66	theme	MoS2-based	258:267	arg1	MoS2-SS-HA					291:300	MoS2-SS-HA	291:300	MoS2-SS-HA	291:300	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	2	66	theme	MoS2-based	258:267	arg1	system					283:288	a novel multifunctional MoS2-based drug delivery system	234:288	a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA)	234:301	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	7	67	theme	ablation	1293:1300	arg1	studies					1302:1308	In vivo tumor ablation studies	1279:1308	In vivo tumor ablation studies	1279:1308	In vivo tumor ablation studies prove a better synergistic therapeutic effect of the joint treatment, compared with either chemotherapy or photothermal therapy alone.					
31217009	1	68	theme	due	155:157	arg1	applications					142:153	biomedical applications	131:153	biomedical applications due to its brilliant photothermal conversion ability	131:206	BACKGROUND Molybdenum disulfide (MoS2) has been widely explored for biomedical applications due to its brilliant photothermal conversion ability.					
31217009	4	69	theme	uniform	688:694	arg1	diameter					696:703	a uniform diameter	686:703	a uniform diameter (ca. 125 nm)	686:716	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	4	69	theme	uniform	688:694	arg1	125 nm					710:715	125 nm	710:715	125 nm	710:715	RESULTS The nanocomposites (MoS2-SS-HA) generated a uniform diameter (ca. 125 nm), exhibited great biocompatibility as well as high stability in physiological solutions, and could be loaded with the insoluble anti-cancer drug erlotinib (Er).					
31217009	6	70	dep	assays	1086:1091	arg1	results					1114:1120	results	1114:1120	results	1114:1120	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	71	dep	cells	1255:1259	arg1	A549					1262:1265	A549	1262:1265	A549	1262:1265	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	71	dep	cells	1255:1259	arg1	cells					1255:1259	drug resistant cells	1240:1259	especially drug resistant cells (A549 and H1975)	1229:1276	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	6	71	dep	cells	1255:1259	arg1	H1975					1271:1275	H1975	1271:1275	H1975	1271:1275	MTT assays and confocal imaging results showed that the MoS2-based nanoplatform could selectively target and kill CD44-positive lung cancer cells, especially drug resistant cells (A549 and H1975).					
31217009	2	72	theme	multifunctional	242:256	arg1	MoS2-SS-HA					291:300	MoS2-SS-HA	291:300	MoS2-SS-HA	291:300	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	2	72	theme	multifunctional	242:256	arg1	system					283:288	a novel multifunctional MoS2-based drug delivery system	234:288	a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA)	234:301	In this paper, we report a novel multifunctional MoS2-based drug delivery system (MoS2-SS-HA).					
31217009	8	73	theme	chemo-photothermal	1584:1601	arg1	therapy					1610:1616	synergistic chemo-photothermal cancer therapy	1572:1616	synergistic chemo-photothermal cancer therapy	1572:1616	CONCLUSION The functionalized MoS2 nanoplatform developed in this work could be a potent system for targeted drug delivery and synergistic chemo-photothermal cancer therapy.					
30407903	3	0	theme	16S	278:280	arg1	sequences					292:300	16S rRNA gene sequences	278:300	16S rRNA gene sequences	278:300	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	5	1	theme	major	697:701	arg1	diphosphatidylglycerol					721:742	diphosphatidylglycerol	721:742	diphosphatidylglycerol	721:742	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	1	theme	major	697:701	arg1	lipids					709:714	The major polar lipids	693:714	The major polar lipids	693:714	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	4	2	contain	contained	632:640	arg1	isolates					623:630	The two isolates	615:630	The two isolates	615:630	The two isolates contained m-diaminopimelic acid within their peptidoglycans.					
30407903	4	2	contain	contained	632:640	arg2	acid					659:662	m-diaminopimelic acid	642:662	m-diaminopimelic acid	642:662	The two isolates contained m-diaminopimelic acid within their peptidoglycans.					
30407903	3	3	theme	rRNA	282:285	arg1	sequences					292:300	16S rRNA gene sequences	278:300	16S rRNA gene sequences	278:300	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	6	4	theme	glucose	905:911	arg1	fermentation					913:924	glucose fermentation	905:924	glucose fermentation	905:924	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	3	5	theme	Blautia	451:457	arg1	5981T					474:478	Blautia stercoris KCTC 5981T	451:478	Blautia stercoris KCTC 5981T	451:478	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	6	dep	%	578:578	arg1	94.2					564:567	94.2	564:567	94.2	564:567	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	6	dep	%	578:578	arg1	93.9 					573:577	93.9 	573:577	93.9 	573:577	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	9	7	theme	type	1114:1117	arg1	N6H1-15T					1129:1136	N6H1-15T	1129:1136	N6H1-15T (=KCTC 15426=JCM 31394)	1129:1160	The type strain is N6H1-15T (=KCTC 15426=JCM 31394).					
30407903	9	7	theme	type	1114:1117	arg1	strain					1119:1124	The type strain	1110:1124	The type strain	1110:1124	The type strain is N6H1-15T (=KCTC 15426=JCM 31394).					
30407903	5	8	dep	C16 	801:804	arg1	%					859:859	16.2 %	854:859	16.2 %	854:859	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	8	dep	C16 	801:804	arg1	C18 					840:843	C18 	840:843	C18 	840:843	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	8	dep	C16 	801:804	arg1	 1cis					845:849	 1cis 9	845:851	C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %)	801:860	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	8	dep	C16 	801:804	arg1	%					815:815	18.5 %	810:815	18.5 %	810:815	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	8	dep	C16 	801:804	arg1	 0					806:807	 0	806:807	 0	806:807	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	8	dep	C16 	801:804	arg1	C16 					819:822	C16 	819:822	C16 	819:822	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	2	9	theme	mature	254:259	arg1	dog					261:263	a mature dog	252:263	a mature dog	252:263	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	7	10	theme	Blautia	1088:1094	arg1	sp					1101:1102	Blautia argi sp	1088:1102	Blautia argi sp	1088:1102	Thus, the polyphasic data suggest that the two new isolates represent a new species, proposed as Blautia argi sp.					
30407903	2	11	theme	coccoid	120:126	arg1	bacterium					153:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	11	theme	coccoid	120:126	arg1	strains					175:181	designated strains N6H1-15T and YH1_16	164:201	designated strains N6H1-15T and YH1_16	164:201	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	3	12	dep	Blautia	346:352	arg1	coccoidesrRNA					354:366	coccoidesrRNA	354:366	coccoidesrRNA	354:366	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	13	theme	producta	489:496	arg1	3695T					512:516	Blautia producta producta KCTC 3695T	481:516	Blautia producta producta KCTC 3695T	481:516	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	14	dep	similarity	589:598	arg1	%					578:578	%	578:578	%	578:578	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	5	15	theme	polar	703:707	arg1	diphosphatidylglycerol					721:742	diphosphatidylglycerol	721:742	diphosphatidylglycerol	721:742	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	15	theme	polar	703:707	arg1	lipids					709:714	The major polar lipids	693:714	The major polar lipids	693:714	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	6	16	theme	44.2 mol	980:987	arg1	G+C content					964:974	the G+C content	960:974	the G+C content	960:974	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	6	16	theme	44.2 mol	980:987	arg1	%					988:988	44.2 mol%	980:988	44.2 mol%	980:988	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	5	17	dep	C16 	819:822	arg1	 0					824:825	 0	824:825	 0	824:825	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	17	dep	C16 	819:822	arg1	%					833:833	18.0 %	828:833	18.0 %	828:833	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	3	18	dep	B.	522:523	arg1	coccoides					525:533	coccoides	525:533	coccoides	525:533	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	2	19	theme	non-motile	108:117	arg1	bacterium					153:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	19	theme	non-motile	108:117	arg1	strains					175:181	designated strains N6H1-15T and YH1_16	164:201	designated strains N6H1-15T and YH1_16	164:201	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	3	20	theme	Blautia	346:352	arg1	group					373:377	the Blautia coccoidesrRNA gene group	342:377	the Blautia coccoidesrRNA gene group (cluster XIVa)	342:392	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	20	theme	Blautia	346:352	arg1	XIVa					388:391	cluster XIVa	380:391	cluster XIVa	380:391	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	6	21	theme	lactic	942:947	arg1	acids					949:953	acetic and lactic acids	931:953	acetic and lactic acids	931:953	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	6	21	theme	lactic	942:947	arg1	products					893:900	The predominant metabolic end products	863:900	The predominant metabolic end products of glucose fermentation	863:924	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	6	22	theme	acetic	931:936	arg1	acids					949:953	acetic and lactic acids	931:953	acetic and lactic acids	931:953	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	6	22	theme	acetic	931:936	arg1	products					893:900	The predominant metabolic end products	863:900	The predominant metabolic end products of glucose fermentation	863:924	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	0	23	theme	argi	8:11	arg1	sp					13:14	Blautia argi sp	0:14	Blautia argi sp.	0:15	Blautia argi sp.					
30407903	1	24	theme	anaerobic	29:37	arg1	bacterium					39:47	a new anaerobic bacterium	23:47	a new anaerobic bacterium	23:47	nov., a new anaerobic bacterium isolated from dog faeces.					
30407903	1	24	theme	anaerobic	29:37	arg1	nov.					17:20	nov.	17:20	nov.	17:20	nov., a new anaerobic bacterium isolated from dog faeces.					
30407903	1	25	theme	dog	63:65	arg1	faeces					67:72	dog faeces	63:72	dog faeces	63:72	nov., a new anaerobic bacterium isolated from dog faeces.					
30407903	7	26	theme	argi	1096:1099	arg1	sp					1101:1102	Blautia argi sp	1088:1102	Blautia argi sp	1088:1102	Thus, the polyphasic data suggest that the two new isolates represent a new species, proposed as Blautia argi sp.					
30407903	3	27	theme	cluster	380:386	arg1	group					373:377	the Blautia coccoidesrRNA gene group	342:377	the Blautia coccoidesrRNA gene group (cluster XIVa)	342:392	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	27	theme	cluster	380:386	arg1	XIVa					388:391	cluster XIVa	380:391	cluster XIVa	380:391	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	0	28	theme	Blautia	0:6	arg1	sp					13:14	Blautia argi sp	0:14	Blautia argi sp.	0:15	Blautia argi sp.					
30407903	9	29	theme	=KCTC	1139:1143	arg1	31394					1155:1159	=KCTC 15426=JCM 31394	1139:1159	=KCTC 15426=JCM 31394	1139:1159	The type strain is N6H1-15T (=KCTC 15426=JCM 31394).					
30407903	9	29	theme	=KCTC	1139:1143	arg1	N6H1-15T					1129:1136	N6H1-15T	1129:1136	N6H1-15T (=KCTC 15426=JCM 31394)	1129:1160	The type strain is N6H1-15T (=KCTC 15426=JCM 31394).					
30407903	6	30	theme	fermentation	913:924	arg1	products					893:900	The predominant metabolic end products	863:900	The predominant metabolic end products of glucose fermentation	863:924	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	6	30	theme	fermentation	913:924	arg1	acids					949:953	acetic and lactic acids	931:953	acetic and lactic acids	931:953	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	9	31	theme	15426=JCM	1145:1153	arg1	31394					1155:1159	=KCTC 15426=JCM 31394	1139:1159	=KCTC 15426=JCM 31394	1139:1159	The type strain is N6H1-15T (=KCTC 15426=JCM 31394).					
30407903	9	31	theme	15426=JCM	1145:1153	arg1	N6H1-15T					1129:1136	N6H1-15T	1129:1136	N6H1-15T (=KCTC 15426=JCM 31394)	1129:1160	The type strain is N6H1-15T (=KCTC 15426=JCM 31394).					
30407903	3	32	theme	producta	498:505	arg1	3695T					512:516	Blautia producta producta KCTC 3695T	481:516	Blautia producta producta KCTC 3695T	481:516	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	5	33	theme	major	778:782	arg1	acids					790:794	the major fatty acids	774:794	the major fatty acids	774:794	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	33	theme	major	778:782	arg1	C16 					801:804	C16 	801:804	C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %)	801:860	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	7	34	theme	polyphasic	1001:1010	arg1	data					1012:1015	the polyphasic data	997:1015	the polyphasic data	997:1015	Thus, the polyphasic data suggest that the two new isolates represent a new species, proposed as Blautia argi sp.					
30407903	2	35	theme	bacterium	153:161	arg1	isolates					79:86	Two isolates	75:86	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16,	75:202	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	3	36	theme	KCTC	507:510	arg1	3695T					512:516	Blautia producta producta KCTC 3695T	481:516	Blautia producta producta KCTC 3695T	481:516	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	5	37	theme	fatty	784:788	arg1	acids					790:794	the major fatty acids	774:794	the major fatty acids	774:794	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	5	37	theme	fatty	784:788	arg1	C16 					801:804	C16 	801:804	C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %)	801:860	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %).					
30407903	3	38	with	related	411:417	arg1	similarity					589:598	96.7, 94.4, 94.2 and 93.9 % sequence similarity	552:598	96.7, 94.4, 94.2 and 93.9 % sequence similarity	552:598	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	2	39	theme	anaerobic	143:151	arg1	bacterium					153:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	39	theme	anaerobic	143:151	arg1	strains					175:181	designated strains N6H1-15T and YH1_16	164:201	designated strains N6H1-15T and YH1_16	164:201	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	3	40	theme	DSM	535:537	arg1	15327T					539:544	B. coccoides DSM 15327T	522:544	B. coccoides DSM 15327T	522:544	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	6	41	theme	end	889:891	arg1	products					893:900	The predominant metabolic end products	863:900	The predominant metabolic end products of glucose fermentation	863:924	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	6	41	theme	end	889:891	arg1	acids					949:953	acetic and lactic acids	931:953	acetic and lactic acids	931:953	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	7	42	theme	new	1063:1065	arg1	species					1067:1073	a new species	1061:1073	a new species	1061:1073	Thus, the polyphasic data suggest that the two new isolates represent a new species, proposed as Blautia argi sp.					
30407903	3	43	theme	B.	522:523	arg1	15327T					539:544	B. coccoides DSM 15327T	522:544	B. coccoides DSM 15327T	522:544	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	44	theme	KCTC	439:442	arg1	5951T					444:448	Blautia hansenii KCTC 5951T	422:448	Blautia hansenii KCTC 5951T	422:448	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	45	theme	gene	368:371	arg1	group					373:377	the Blautia coccoidesrRNA gene group	342:377	the Blautia coccoidesrRNA gene group (cluster XIVa)	342:392	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	45	theme	gene	368:371	arg1	XIVa					388:391	cluster XIVa	380:391	cluster XIVa	380:391	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	46	theme	sequence	580:587	arg1	similarity					589:598	96.7, 94.4, 94.2 and 93.9 % sequence similarity	552:598	96.7, 94.4, 94.2 and 93.9 % sequence similarity	552:598	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	7	47	theme	new	1038:1040	arg1	isolates					1042:1049	the two new isolates	1030:1049	the two new isolates	1030:1049	Thus, the polyphasic data suggest that the two new isolates represent a new species, proposed as Blautia argi sp.					
30407903	6	48	theme	predominant	867:877	arg1	products					893:900	The predominant metabolic end products	863:900	The predominant metabolic end products of glucose fermentation	863:924	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	6	48	theme	predominant	867:877	arg1	acids					949:953	acetic and lactic acids	931:953	acetic and lactic acids	931:953	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	3	49	theme	Blautia	422:428	arg1	5951T					444:448	Blautia hansenii KCTC 5951T	422:448	Blautia hansenii KCTC 5951T	422:448	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	6	50	theme	metabolic	879:887	arg1	products					893:900	The predominant metabolic end products	863:900	The predominant metabolic end products of glucose fermentation	863:924	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	6	50	theme	metabolic	879:887	arg1	acids					949:953	acetic and lactic acids	931:953	acetic and lactic acids	931:953	The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%.					
30407903	2	51	theme	designated	164:173	arg1	bacterium					153:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	51	theme	designated	164:173	arg1	strains					175:181	designated strains N6H1-15T and YH1_16	164:201	designated strains N6H1-15T and YH1_16	164:201	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	51	theme	designated	164:173	arg1	YH1_16					196:201	YH1_16	196:201	YH1_16	196:201	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	51	theme	designated	164:173	arg1	N6H1-15T					183:190	N6H1-15T	183:190	N6H1-15T	183:190	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	52	theme	faecal	223:228	arg1	samples					230:236	faecal samples	223:236	faecal samples obtained from a mature dog	223:263	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	3	53	theme	hansenii	430:437	arg1	5951T					444:448	Blautia hansenii KCTC 5951T	422:448	Blautia hansenii KCTC 5951T	422:448	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	4	54	theme	m-diaminopimelic	642:657	arg1	acid					659:662	m-diaminopimelic acid	642:662	m-diaminopimelic acid	642:662	The two isolates contained m-diaminopimelic acid within their peptidoglycans.					
30407903	3	55	theme	gene	287:290	arg1	sequences					292:300	16S rRNA gene sequences	278:300	16S rRNA gene sequences	278:300	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	56	theme	Blautia	481:487	arg1	3695T					512:516	Blautia producta producta KCTC 3695T	481:516	Blautia producta producta KCTC 3695T	481:516	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	3	57	theme	stercoris	459:467	arg1	5981T					474:478	Blautia stercoris KCTC 5981T	451:478	Blautia stercoris KCTC 5981T	451:478	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	2	58	attach	isolated	209:216	arg2	isolates					79:86	Two isolates	75:86	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16,	75:202	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	58	attach	isolated	209:216	arg1	samples					230:236	faecal samples	223:236	faecal samples obtained from a mature dog	223:263	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	3	59	theme	sequences	292:300	arg1	Analysis					266:273	Analysis	266:273	Analysis of 16S rRNA gene sequences	266:300	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	2	60	theme	Gram-positive	93:105	arg1	bacterium					153:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium	91:161	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	60	theme	Gram-positive	93:105	arg1	strains					175:181	designated strains N6H1-15T and YH1_16	164:201	designated strains N6H1-15T and YH1_16	164:201	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	1	61	theme	new	25:27	arg1	bacterium					39:47	a new anaerobic bacterium	23:47	a new anaerobic bacterium	23:47	nov., a new anaerobic bacterium isolated from dog faeces.					
30407903	1	61	theme	new	25:27	arg1	nov.					17:20	nov.	17:20	nov.	17:20	nov., a new anaerobic bacterium isolated from dog faeces.					
30407903	3	62	theme	KCTC	469:472	arg1	5981T					474:478	Blautia stercoris KCTC 5981T	451:478	Blautia stercoris KCTC 5981T	451:478	Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951T, Blautia stercoris KCTC 5981T, Blautia producta producta KCTC 3695T and B. coccoides DSM 15327T, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively.					
30407903	2	63	dep	strains	175:181	arg1	strains					175:181	designated strains N6H1-15T and YH1_16	164:201	designated strains N6H1-15T and YH1_16	164:201	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	63	dep	strains	175:181	arg1	YH1_16					196:201	YH1_16	196:201	YH1_16	196:201	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30407903	2	63	dep	strains	175:181	arg1	N6H1-15T					183:190	N6H1-15T	183:190	N6H1-15T	183:190	Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15T and YH1_16, were isolated from faecal samples obtained from a mature dog.					
30361878	0	0	theme	Bacillus	98:105	arg1	BD92					118:121	Bacillus sonorensis BD92	98:121	Bacillus sonorensis BD92 with their application in biomass saccharification	98:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	6	1	theme	experimental	1082:1093	arg1	runs					1095:1098	only 20 experimental runs	1074:1098	only 20 experimental runs	1074:1098	This statistical model was efficient because only 20 experimental runs were necessary to assess the highest production conditions, and the model accuracy was very satisfactory as coefficient of determination (R2) was 0.95 and 0.89 for endo-glucanase and xylanase, respectively.					
30361878	9	2	theme	sonorensis	1689:1698	arg1	BD92					1700:1703	Bacillus sonorensis BD92	1680:1703	Bacillus sonorensis BD92	1680:1703	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	8	3	theme	reducing	1584:1591	arg1	xylose					1613:1618	xylose	1613:1618	xylose	1613:1618	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	8	3	theme	reducing	1584:1591	arg1	glucose					1601:1607	glucose	1601:1607	glucose	1601:1607	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	8	3	theme	reducing	1584:1591	arg1	sugars					1593:1598	reducing sugars	1584:1598	reducing sugars (glucose and xylose)	1584:1619	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	8	4	theme	enzymatic	1539:1547	arg1	saccharification					1549:1564	enzymatic saccharification	1539:1564	enzymatic saccharification	1539:1564	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	9	5	theme	precise	1889:1895	arg1	conditions					1921:1930	precise statistically optimized conditions	1889:1930	precise statistically optimized conditions	1889:1930	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	2	6	theme	xylanase	401:408	arg1	co-production					365:377	co-production	365:377	co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92	365:438	This study reports statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92.					
30361878	1	7	theme	industrial	276:285	arg1	processes					287:295	industrial processes	276:295	industrial processes	276:295	Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes.					
30361878	9	8	theme	plant	1820:1824	arg1	biomass					1826:1832	plant biomass	1820:1832	plant biomass	1820:1832	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	7	9	theme	wheat	1404:1408	arg1	bran					1410:1413	wheat bran	1404:1413	wheat bran	1404:1413	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	2	10	theme	statistical	317:327	arg1	optimization					348:359	statistical based experimental optimization	317:359	statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92	317:438	This study reports statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92.					
30361878	5	11	dep	endo-glucanase	943:956	arg1	the					931:933	the	931:933	the	931:933	The maximum endo-glucanase and xylanase production obtained was 1.46 and 5.69 U/mL, respectively, while the minimum endo-glucanase and xylanase production obtained was 0.66 and 0.25 U/mL, respectively.					
30361878	4	12	theme	extract	796:802	arg1	15 g/L					804:809	yeast extract 15 g/L	790:809	yeast extract 15 g/L	790:809	The optimum co-production conditions for endo-glucanase and xylanase were as follows: carboxymethyl cellulose (CMC) 20 g/L, yeast extract 15 g/L, and time 72 h.					
30361878	6	13	theme	determination	1223:1235	arg1	coefficient					1208:1218	coefficient	1208:1218	coefficient of determination (R2)	1208:1240	This statistical model was efficient because only 20 experimental runs were necessary to assess the highest production conditions, and the model accuracy was very satisfactory as coefficient of determination (R2) was 0.95 and 0.89 for endo-glucanase and xylanase, respectively.					
30361878	4	14	theme	cellulose	766:774	arg1	20 g/L					782:787	carboxymethyl cellulose (CMC) 20 g/L	752:787	carboxymethyl cellulose (CMC) 20 g/L	752:787	The optimum co-production conditions for endo-glucanase and xylanase were as follows: carboxymethyl cellulose (CMC) 20 g/L, yeast extract 15 g/L, and time 72 h.					
30361878	5	15	dep	endo-glucanase	839:852	arg1	The					827:829	The	827:829	The	827:829	The maximum endo-glucanase and xylanase production obtained was 1.46 and 5.69 U/mL, respectively, while the minimum endo-glucanase and xylanase production obtained was 0.66 and 0.25 U/mL, respectively.					
30361878	5	16	theme	xylanase	858:865	arg1	production					867:876	xylanase production	858:876	xylanase production	858:876	The maximum endo-glucanase and xylanase production obtained was 1.46 and 5.69 U/mL, respectively, while the minimum endo-glucanase and xylanase production obtained was 0.66 and 0.25 U/mL, respectively.					
30361878	7	17	theme	biomass	1395:1401	arg1	saccharification					1359:1374	saccharification	1359:1374	saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk)	1359:1457	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	3	18	theme	composite	494:502	arg1	CCD					512:514	CCD	512:514	CCD	512:514	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	3	18	theme	composite	494:502	arg1	design					504:509	central composite design	486:509	central composite design (CCD) with full factorial experiments (23)	486:552	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	9	19	theme	value-added	1839:1849	arg1	products					1851:1858	value-added products	1839:1858	value-added products of economic importance	1839:1881	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	0	20	from	co-production	48:60	arg1	BD92					118:121	Bacillus sonorensis BD92	98:121	Bacillus sonorensis BD92 with their application in biomass saccharification	98:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	7	21	theme	lignocellulosic	1379:1393	arg1	biomass					1395:1401	lignocellulosic biomass	1379:1401	lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk)	1379:1457	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	3	22	theme	Response	441:448	arg1	RSM					471:473	RSM	471:473	RSM	471:473	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	3	22	theme	Response	441:448	arg1	methodology					458:468	Response surface methodology	441:468	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23)	441:552	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	5	23	theme	xylanase	962:969	arg1	production					971:980	xylanase production	962:980	xylanase production	962:980	The maximum endo-glucanase and xylanase production obtained was 1.46 and 5.69 U/mL, respectively, while the minimum endo-glucanase and xylanase production obtained was 0.66 and 0.25 U/mL, respectively.					
30361878	7	24	theme	potential	1316:1324	arg1	application					1326:1336	potential application	1316:1336	potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk)	1316:1457	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	0	25	dep	Bacillus	98:105	arg1	sonorensis					107:116	sonorensis	107:116	sonorensis	107:116	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	9	26	theme	producer	1776:1783	arg1	useful					1799:1804	useful	1799:1804	useful	1799:1804	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	9	26	theme	producer	1776:1783	arg1	promise					1715:1721	a promise	1713:1721	a promise	1713:1721	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	9	26	theme	producer	1776:1783	arg1	source					1728:1733	a source	1726:1733	a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions	1726:1930	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	6	27	theme	production	1137:1146	arg1	conditions					1148:1157	the highest production conditions	1125:1157	the highest production conditions	1125:1157	This statistical model was efficient because only 20 experimental runs were necessary to assess the highest production conditions, and the model accuracy was very satisfactory as coefficient of determination (R2) was 0.95 and 0.89 for endo-glucanase and xylanase, respectively.					
30361878	3	28	theme	surface	450:456	arg1	RSM					471:473	RSM	471:473	RSM	471:473	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	3	28	theme	surface	450:456	arg1	methodology					458:468	Response surface methodology	441:468	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23)	441:552	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	0	29	with	BD92	118:121	arg1	application					134:144	their application	128:144	their application in biomass saccharification	128:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	4	30	theme	yeast	790:794	arg1	15 g/L					804:809	yeast extract 15 g/L	790:809	yeast extract 15 g/L	790:809	The optimum co-production conditions for endo-glucanase and xylanase were as follows: carboxymethyl cellulose (CMC) 20 g/L, yeast extract 15 g/L, and time 72 h.					
30361878	3	31	theme	central	486:492	arg1	CCD					512:514	CCD	512:514	CCD	512:514	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	3	31	theme	central	486:492	arg1	design					504:509	central composite design	486:509	central composite design (CCD) with full factorial experiments (23)	486:552	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	9	32	theme	economic	1863:1870	arg1	importance					1872:1881	economic importance	1863:1881	economic importance	1863:1881	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	6	33	theme	statistical	1034:1044	arg1	model					1046:1050	This statistical model	1029:1050	This statistical model	1029:1050	This statistical model was efficient because only 20 experimental runs were necessary to assess the highest production conditions, and the model accuracy was very satisfactory as coefficient of determination (R2) was 0.95 and 0.89 for endo-glucanase and xylanase, respectively.					
30361878	2	34	theme	experimental	335:346	arg1	optimization					348:359	statistical based experimental optimization	317:359	statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92	317:438	This study reports statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92.					
30361878	1	35	theme	high	220:223	arg1	demand					236:241	high industrial demand	220:241	high industrial demand	220:241	Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes.					
30361878	0	36	theme	experimental	18:29	arg1	optimization					31:42	experimental optimization	18:42	experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification	18:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	9	37	theme	potential	1738:1746	arg1	endo-glucanase					1748:1761	potential endo-glucanase	1738:1761	potential endo-glucanase	1738:1761	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	1	38	contain	have	215:218	arg1	xylanase					206:213	xylanase	206:213	xylanase	206:213	Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes.					
30361878	1	38	contain	have	215:218	arg1	Endo-glucanase					175:188	Endo-glucanase	175:188	Endo-glucanase (cellulase)	175:200	Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes.					
30361878	1	38	contain	have	215:218	arg2	demand					236:241	high industrial demand	220:241	high industrial demand	220:241	Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes.					
30361878	1	38	contain	have	215:218	arg1	cellulase					191:199	cellulase	191:199	cellulase	191:199	Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes.					
30361878	2	39	theme	based	329:333	arg1	optimization					348:359	statistical based experimental optimization	317:359	statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92	317:438	This study reports statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92.					
30361878	1	40	theme	industrial	225:234	arg1	demand					236:241	high industrial demand	220:241	high industrial demand	220:241	Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes.					
30361878	4	41	theme	CMC	777:779	arg1	20 g/L					782:787	carboxymethyl cellulose (CMC) 20 g/L	752:787	carboxymethyl cellulose (CMC) 20 g/L	752:787	The optimum co-production conditions for endo-glucanase and xylanase were as follows: carboxymethyl cellulose (CMC) 20 g/L, yeast extract 15 g/L, and time 72 h.					
30361878	9	42	theme	endo-glucanase	1748:1761	arg1	useful					1799:1804	useful	1799:1804	useful	1799:1804	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	9	42	theme	endo-glucanase	1748:1761	arg1	promise					1715:1721	a promise	1713:1721	a promise	1713:1721	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	9	42	theme	endo-glucanase	1748:1761	arg1	source					1728:1733	a source	1726:1733	a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions	1726:1930	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	7	43	dep	biomass	1395:1401	arg1	straw					1422:1426	wheat straw	1416:1426	wheat straw	1416:1426	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	7	43	dep	biomass	1395:1401	arg1	straw					1434:1438	rice straw	1429:1438	rice straw	1429:1438	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	7	43	dep	biomass	1395:1401	arg1	stalk					1452:1456	cotton stalk	1445:1456	cotton stalk	1445:1456	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	7	43	dep	biomass	1395:1401	arg1	bran					1410:1413	wheat bran	1404:1413	wheat bran	1404:1413	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	6	44	theme	highest	1129:1135	arg1	conditions					1148:1157	the highest production conditions	1125:1157	the highest production conditions	1125:1157	This statistical model was efficient because only 20 experimental runs were necessary to assess the highest production conditions, and the model accuracy was very satisfactory as coefficient of determination (R2) was 0.95 and 0.89 for endo-glucanase and xylanase, respectively.					
30361878	4	45	theme	carboxymethyl	752:764	arg1	20 g/L					782:787	carboxymethyl cellulose (CMC) 20 g/L	752:787	carboxymethyl cellulose (CMC) 20 g/L	752:787	The optimum co-production conditions for endo-glucanase and xylanase were as follows: carboxymethyl cellulose (CMC) 20 g/L, yeast extract 15 g/L, and time 72 h.					
30361878	9	46	theme	importance	1872:1881	arg1	products					1851:1858	value-added products	1839:1858	value-added products of economic importance	1839:1881	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	0	47	theme	biomass	149:155	arg1	saccharification					157:172	biomass saccharification	149:172	biomass saccharification	149:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	3	48	theme	xylanase	656:663	arg1	co-production					620:632	co-production	620:632	co-production of endo-glucanase and xylanase	620:663	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	0	49	theme	endo-glucanase	65:78	arg1	co-production					48:60	co-production	48:60	co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification	48:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	5	50	theme	minimum	935:941	arg1	endo-glucanase					943:956	minimum endo-glucanase	935:956	minimum endo-glucanase	935:956	The maximum endo-glucanase and xylanase production obtained was 1.46 and 5.69 U/mL, respectively, while the minimum endo-glucanase and xylanase production obtained was 0.66 and 0.25 U/mL, respectively.					
30361878	9	51	theme	xylanase	1767:1774	arg1	producer					1776:1783	xylanase producer	1767:1783	xylanase producer	1767:1783	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	7	52	theme	rice	1429:1432	arg1	straw					1434:1438	rice straw	1429:1438	rice straw	1429:1438	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	2	53	from	BD92	435:438	arg1	co-production					365:377	co-production	365:377	co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92	365:438	This study reports statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92.					
30361878	8	54	theme	sugars	1593:1598	arg1	xylose					1613:1618	xylose	1613:1618	xylose	1613:1618	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	8	54	theme	sugars	1593:1598	arg1	glucose					1601:1607	glucose	1601:1607	glucose	1601:1607	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	8	54	theme	sugars	1593:1598	arg1	amount					1574:1579	the amount	1570:1579	the amount of reducing sugars (glucose and xylose)	1570:1619	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	8	54	theme	sugars	1593:1598	arg1	sugars					1593:1598	reducing sugars	1584:1598	reducing sugars (glucose and xylose)	1584:1619	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	1	55	from	application	261:271	arg1	processes					287:295	industrial processes	276:295	industrial processes	276:295	Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes.					
30361878	3	56	theme	endo-glucanase	637:650	arg1	co-production					620:632	co-production	620:632	co-production of endo-glucanase and xylanase	620:663	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	2	57	theme	endo-glucanase	382:395	arg1	co-production					365:377	co-production	365:377	co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92	365:438	This study reports statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92.					
30361878	7	58	theme	wheat	1416:1420	arg1	straw					1422:1426	wheat straw	1416:1426	wheat straw	1416:1426	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	5	59	theme	maximum	831:837	arg1	endo-glucanase					839:852	maximum endo-glucanase	831:852	maximum endo-glucanase	831:852	The maximum endo-glucanase and xylanase production obtained was 1.46 and 5.69 U/mL, respectively, while the minimum endo-glucanase and xylanase production obtained was 0.66 and 0.25 U/mL, respectively.					
30361878	9	60	theme	Bacillus	1680:1687	arg1	BD92					1700:1703	Bacillus sonorensis BD92	1680:1703	Bacillus sonorensis BD92	1680:1703	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	3	61	theme	full	522:525	arg1	experiments					537:547	full factorial experiments	522:547	full factorial experiments (23)	522:552	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	3	61	theme	full	522:525	arg1	23					550:551	23	550:551	23	550:551	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	7	62	theme	cotton	1445:1450	arg1	stalk					1452:1456	cotton stalk	1445:1456	cotton stalk	1445:1456	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	8	63	theme	incubation	1648:1657	arg1	time					1659:1662	incubation time	1648:1662	incubation time	1648:1662	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	3	64	with	design	504:509	arg1	experiments					537:547	full factorial experiments	522:547	full factorial experiments (23)	522:552	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	3	64	with	design	504:509	arg1	23					550:551	23	550:551	23	550:551	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	0	65	from	BD92	118:121	arg1	co-production					48:60	co-production	48:60	co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification	48:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	0	65	from	BD92	118:121	arg1	xylanase					84:91	xylanase	84:91	xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification	84:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	0	65	from	BD92	118:121	arg1	endo-glucanase					65:78	endo-glucanase	65:78	endo-glucanase	65:78	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	4	66	theme	time	816:819	arg1	72 h					821:824	time 72 h	816:824	time 72 h	816:824	The optimum co-production conditions for endo-glucanase and xylanase were as follows: carboxymethyl cellulose (CMC) 20 g/L, yeast extract 15 g/L, and time 72 h.					
30361878	3	67	theme	factorial	527:535	arg1	experiments					537:547	full factorial experiments	522:547	full factorial experiments (23)	522:552	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	3	67	theme	factorial	527:535	arg1	23					550:551	23	550:551	23	550:551	Response surface methodology (RSM) involving central composite design (CCD) with full factorial experiments (23) was applied to elucidate the components that significantly affect co-production of endo-glucanase and xylanase.					
30361878	0	68	theme	xylanase	84:91	arg1	co-production					48:60	co-production	48:60	co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification	48:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
30361878	6	69	theme	model	1168:1172	arg1	accuracy					1174:1181	the model accuracy	1164:1181	the model accuracy	1164:1181	This statistical model was efficient because only 20 experimental runs were necessary to assess the highest production conditions, and the model accuracy was very satisfactory as coefficient of determination (R2) was 0.95 and 0.89 for endo-glucanase and xylanase, respectively.					
30361878	6	69	theme	model	1168:1172	arg1	satisfactory					1192:1203	satisfactory	1192:1203	satisfactory	1192:1203	This statistical model was efficient because only 20 experimental runs were necessary to assess the highest production conditions, and the model accuracy was very satisfactory as coefficient of determination (R2) was 0.95 and 0.89 for endo-glucanase and xylanase, respectively.					
30361878	9	70	theme	optimized	1911:1919	arg1	conditions					1921:1930	precise statistically optimized conditions	1889:1930	precise statistically optimized conditions	1889:1930	In conclusion, Bacillus sonorensis BD92 reveals a promise as a source of potential endo-glucanase and xylanase producer that could be useful for degrading plant biomass into value-added products of economic importance using precise statistically optimized conditions.					
30361878	4	71	theme	co-production	678:690	arg1	conditions					692:701	The optimum co-production conditions	666:701	The optimum co-production conditions for endo-glucanase and xylanase	666:733	The optimum co-production conditions for endo-glucanase and xylanase were as follows: carboxymethyl cellulose (CMC) 20 g/L, yeast extract 15 g/L, and time 72 h.					
30361878	2	72	theme	sonorensis	424:433	arg1	BD92					435:438	Bacillus sonorensis BD92	415:438	Bacillus sonorensis BD92	415:438	This study reports statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92.					
30361878	1	73	theme	vast	256:259	arg1	application					261:271	their vast application	250:271	their vast application in industrial processes	250:295	Endo-glucanase (cellulase) and xylanase have high industrial demand due to their vast application in industrial processes.					
30361878	8	74	dep	sugars	1593:1598	arg1	xylose					1613:1618	xylose	1613:1618	xylose	1613:1618	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	8	74	dep	sugars	1593:1598	arg1	glucose					1601:1607	glucose	1601:1607	glucose	1601:1607	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	8	74	dep	sugars	1593:1598	arg1	sugars					1593:1598	reducing sugars	1584:1598	reducing sugars (glucose and xylose)	1584:1619	The results revealed that the biomass was susceptible to enzymatic saccharification and the amount of reducing sugars (glucose and xylose) increased with increase in incubation time.					
30361878	4	75	theme	optimum	670:676	arg1	conditions					692:701	The optimum co-production conditions	666:701	The optimum co-production conditions for endo-glucanase and xylanase	666:733	The optimum co-production conditions for endo-glucanase and xylanase were as follows: carboxymethyl cellulose (CMC) 20 g/L, yeast extract 15 g/L, and time 72 h.					
30361878	2	76	theme	Bacillus	415:422	arg1	BD92					435:438	Bacillus sonorensis BD92	415:438	Bacillus sonorensis BD92	415:438	This study reports statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92.					
30361878	7	77	theme	enzymes	1347:1353	arg1	application					1326:1336	potential application	1316:1336	potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk)	1316:1457	Further, potential application of these enzymes for saccharification of lignocellulosic biomass (wheat bran, wheat straw, rice straw, and cotton stalk) was also investigated.					
30361878	0	78	from	application	134:144	arg1	saccharification					157:172	biomass saccharification	149:172	biomass saccharification	149:172	Statistical based experimental optimization for co-production of endo-glucanase and xylanase from Bacillus sonorensis BD92 with their application in biomass saccharification.					
31619645	3	0	theme	trace	395:399	arg1	amounts					401:407	only trace amounts	390:407	only trace amounts of DF	390:413	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	3	0	theme	trace	395:399	arg1	DF					412:413	DF	412:413	DF	412:413	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	4	1	theme	foods	555:559	arg1	consumption					532:542	The excessive consumption	518:542	The excessive consumption of high GI foods	518:559	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	7	2	theme	cell	1144:1147	arg1	components					1176:1185	the cell wall derived-dietary fibre components	1140:1185	the cell wall derived-dietary fibre components	1140:1185	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	4	3	theme	diseases	618:625	arg1	range					601:605	a range	599:605	a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer	599:706	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	3	theme	diseases	618:625	arg1	diabetes					644:651	type-2 diabetes	637:651	type-2 diabetes	637:651	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	3	theme	diseases	618:625	arg1	disease					669:675	cardiovascular disease	654:675	cardiovascular disease (CVD)	654:681	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	3	theme	diseases	618:625	arg1	types					692:696	some types	687:696	some types of cancer	687:706	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	3	4	theme	human	446:450	arg1	tract					469:473	the human gastrointestinal tract	442:473	the human gastrointestinal tract	442:473	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	4	5	theme	chronic	610:616	arg1	diseases					618:625	chronic diseases	610:625	chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer	610:706	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	5	theme	chronic	610:616	arg1	diabetes					644:651	type-2 diabetes	637:651	type-2 diabetes	637:651	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	5	theme	chronic	610:616	arg1	disease					669:675	cardiovascular disease	654:675	cardiovascular disease (CVD)	654:681	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	5	theme	chronic	610:616	arg1	types					692:696	some types	687:696	some types of cancer	687:706	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	6	theme	increased	580:588	arg1	risks					590:594	increased risks	580:594	increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer	580:706	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	7	7	theme	acceptable	1068:1077	arg1	levels					1079:1084	acceptable levels	1068:1084	acceptable levels of resistant starch (RS)	1068:1109	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	4	8	theme	type-2	637:642	arg1	diabetes					644:651	type-2 diabetes	637:651	type-2 diabetes	637:651	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	1	9	theme	most	137:140	arg1	countries					164:172	most South East (SE) Asian countries	137:172	most South East (SE) Asian countries	137:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	5	10	theme	conditions	745:754	arg1	incidence					726:734	the incidence	722:734	the incidence of these conditions	722:754	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	1	11	theme	Soft	71:74	arg1	rice					85:88	Soft textured rice	71:88	Soft textured rice	71:88	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	1	11	theme	Soft	71:74	arg1	source					103:108	the major source	93:108	the major source of calories in the diet of most South East (SE) Asian countries	93:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	1	12	theme	South	142:146	arg1	countries					164:172	most South East (SE) Asian countries	137:172	most South East (SE) Asian countries	137:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	0	13	theme	Rice	10:13	arg1	Content					29:35	Rice Dietary Fibre Content	10:35	Rice Dietary Fibre Content	10:35	Improving Rice Dietary Fibre Content and Composition for Human Health.					
31619645	6	14	theme	rice	969:972	arg1	lines					974:978	rice lines	969:978	rice lines in which high energy content is combined with low GI	969:1031	It is therefore necessary to develop rice lines in which high energy content is combined with low GI.					
31619645	4	15	theme	cancer	701:706	arg1	CVD					678:680	CVD	678:680	CVD	678:680	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	15	theme	cancer	701:706	arg1	diabetes					644:651	type-2 diabetes	637:651	type-2 diabetes	637:651	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	15	theme	cancer	701:706	arg1	disease					669:675	cardiovascular disease	654:675	cardiovascular disease (CVD)	654:681	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	15	theme	cancer	701:706	arg1	types					692:696	some types	687:696	some types of cancer	687:706	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	1	16	theme	textured	76:83	arg1	rice					85:88	Soft textured rice	71:88	Soft textured rice	71:88	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	1	16	theme	textured	76:83	arg1	source					103:108	the major source	93:108	the major source of calories in the diet of most South East (SE) Asian countries	93:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	1	17	theme	East	148:151	arg1	countries					164:172	most South East (SE) Asian countries	137:172	most South East (SE) Asian countries	137:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	3	18	theme	gastrointestinal	452:467	arg1	tract					469:473	the human gastrointestinal tract	442:473	the human gastrointestinal tract	442:473	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	5	19	from	prevalence	852:861	arg1	Asia					881:884	SE Asia	878:884	SE Asia alone predicted to reach 120 million by 2030	878:929	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	0	20	theme	Fibre	23:27	arg1	Content					29:35	Rice Dietary Fibre Content	10:35	Rice Dietary Fibre Content	10:35	Improving Rice Dietary Fibre Content and Composition for Human Health.					
31619645	1	21	from	source	103:108	arg1	diet					129:132	the diet	125:132	the diet of most South East (SE) Asian countries	125:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	5	22	theme	white	798:802	arg1	rice					804:807	white rice	798:807	white rice	798:807	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	5	22	theme	white	798:802	arg1	food					823:826	the staple food	812:826	the staple food	812:826	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	1	23	theme	SE	154:155	arg1	countries					164:172	most South East (SE) Asian countries	137:172	most South East (SE) Asian countries	137:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	0	24	theme	Dietary	15:21	arg1	Content					29:35	Rice Dietary Fibre Content	10:35	Rice Dietary Fibre Content	10:35	Improving Rice Dietary Fibre Content and Composition for Human Health.					
31619645	5	25	theme	staple	816:821	arg1	Asia					837:840	Asia	837:840	notably Asia	829:840	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	5	25	theme	staple	816:821	arg1	rice					804:807	white rice	798:807	white rice	798:807	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	5	25	theme	staple	816:821	arg1	food					823:826	the staple food	812:826	the staple food	812:826	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	4	26	theme	range	601:605	arg1	risks					590:594	increased risks	580:594	increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer	580:706	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	7	27	theme	derived-dietary	1154:1168	arg1	components					1176:1185	the cell wall derived-dietary fibre components	1140:1185	the cell wall derived-dietary fibre components	1140:1185	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	6	28	theme	energy	994:999	arg1	content					1001:1007	high energy content	989:1007	high energy content	989:1007	It is therefore necessary to develop rice lines in which high energy content is combined with low GI.					
31619645	1	29	theme	Asian	158:162	arg1	countries					164:172	most South East (SE) Asian countries	137:172	most South East (SE) Asian countries	137:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	4	30	theme	excessive	522:530	arg1	consumption					532:542	The excessive consumption	518:542	The excessive consumption of high GI foods	518:559	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	5	31	with	food	823:826	arg1	prevalence					852:861	the prevalence	848:861	the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030	848:929	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	7	32	theme	fibre	1170:1174	arg1	components					1176:1185	the cell wall derived-dietary fibre components	1140:1185	the cell wall derived-dietary fibre components	1140:1185	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	1	33	theme	countries	164:172	arg1	diet					129:132	the diet	125:132	the diet of most South East (SE) Asian countries	125:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	6	34	theme	low	1026:1028	arg1	GI					1030:1031	low GI	1026:1031	low GI	1026:1031	It is therefore necessary to develop rice lines in which high energy content is combined with low GI.					
31619645	1	35	theme	major	97:101	arg1	rice					85:88	Soft textured rice	71:88	Soft textured rice	71:88	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	1	35	theme	major	97:101	arg1	source					103:108	the major source	93:108	the major source of calories in the diet of most South East (SE) Asian countries	93:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	5	36	theme	diabetes	866:873	arg1	prevalence					852:861	the prevalence	848:861	the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030	848:929	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	7	37	theme	wall	1149:1152	arg1	components					1176:1185	the cell wall derived-dietary fibre components	1140:1185	the cell wall derived-dietary fibre components	1140:1185	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	4	38	theme	cardiovascular	654:667	arg1	CVD					678:680	CVD	678:680	CVD	678:680	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	4	38	theme	cardiovascular	654:667	arg1	disease					669:675	cardiovascular disease	654:675	cardiovascular disease (CVD)	654:681	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	3	39	theme	glycaemic	496:504	arg1	GI					513:514	GI	513:514	GI	513:514	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	3	39	theme	glycaemic	496:504	arg1	index					506:510	a high glycaemic index	489:510	a high glycaemic index (GI)	489:515	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	4	40	theme	high	547:550	arg1	foods					555:559	high GI foods	547:559	high GI foods	547:559	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	3	41	theme	%	376:376	arg1	starch					378:383	over 90% starch	369:383	over 90% starch	369:383	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	7	42	theme	components	1176:1185	arg1	content					1129:1135	an increased content	1116:1135	an increased content of the cell wall derived-dietary fibre components	1116:1185	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	5	43	theme	SE	878:879	arg1	Asia					881:884	SE Asia	878:884	SE Asia alone predicted to reach 120 million by 2030	878:929	Furthermore, the incidence of these conditions is dramatically increasing in areas where white rice is the staple food, notably Asia, with the prevalence of diabetes in SE Asia alone predicted to reach 120 million by 2030.					
31619645	6	44	theme	high	989:992	arg1	content					1001:1007	high energy content	989:1007	high energy content	989:1007	It is therefore necessary to develop rice lines in which high energy content is combined with low GI.					
31619645	1	45	theme	calories	113:120	arg1	rice					85:88	Soft textured rice	71:88	Soft textured rice	71:88	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	1	45	theme	calories	113:120	arg1	source					103:108	the major source	93:108	the major source of calories in the diet of most South East (SE) Asian countries	93:172	Soft textured rice is the major source of calories in the diet of most South East (SE) Asian countries.					
31619645	7	46	theme	resistant	1089:1097	arg1	starch					1099:1104	resistant starch	1089:1104	resistant starch (RS)	1089:1109	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	7	46	theme	resistant	1089:1097	arg1	RS					1107:1108	RS	1107:1108	RS	1107:1108	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	7	47	theme	increased	1119:1127	arg1	content					1129:1135	an increased content	1116:1135	an increased content of the cell wall derived-dietary fibre components	1116:1185	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	7	48	theme	starch	1099:1104	arg1	levels					1079:1084	acceptable levels	1068:1084	acceptable levels of resistant starch (RS)	1068:1109	This may be achieved by combining acceptable levels of resistant starch (RS) with an increased content of the cell wall derived-dietary fibre components.					
31619645	4	49	theme	GI	552:553	arg1	foods					555:559	high GI foods	547:559	high GI foods	547:559	The excessive consumption of high GI foods is associated with increased risks of a range of chronic diseases including type-2 diabetes, cardiovascular disease (CVD) and some types of cancer.					
31619645	3	50	theme	white	348:352	arg1	rice					354:357	white rice	348:357	white rice	348:357	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	0	51	theme	Human	57:61	arg1	Health					63:68	Human Health	57:68	Human Health	57:68	Improving Rice Dietary Fibre Content and Composition for Human Health.					
31619645	3	52	theme	high	491:494	arg1	GI					513:514	GI	513:514	GI	513:514	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	3	52	theme	high	491:494	arg1	index					506:510	a high glycaemic index	489:510	a high glycaemic index (GI)	489:515	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	3	53	theme	DF	412:413	arg1	amounts					401:407	only trace amounts	390:407	only trace amounts of DF	390:413	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
31619645	3	53	theme	DF	412:413	arg1	DF					412:413	DF	412:413	DF	412:413	Consequently, white rice comprises over 90% starch with only trace amounts of DF and is rapidly digested in the human gastrointestinal tract, resulting in a high glycaemic index (GI).					
30199106	15	0	theme	ready-to-eat	2409:2420	arg1	fruit					2438:2442	ready-to-eat dried blueberry fruit	2409:2442	ready-to-eat dried blueberry fruit	2409:2442	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	3	1	theme	procedure	587:595	arg1	optimization					551:562	optimization	551:562	two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying)	526:747	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	15	2	theme	bioactive	2551:2559	arg1	compounds					2561:2569	natural bioactive compounds	2543:2569	natural bioactive compounds	2543:2569	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	1	3	theme	highbush	243:250	arg1	cultivars					262:270	four highbush blueberry cultivars	238:270	four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot")	238:322	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	7	4	theme	positive	1282:1289	arg1	influence					1291:1299	positive influence	1282:1299	positive influence	1282:1299	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	3	5	theme	main	606:609	arg1	procedure					587:595	osmotic dehydration procedure	567:595	osmotic dehydration procedure	567:595	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	3	5	theme	main	606:609	arg1	experiment					611:620	the main experiment	602:620	(2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying)	598:747	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	5	6	from	fruit	977:981	arg1	dehydration					882:892	The osmotic dehydration	870:892	The osmotic dehydration	870:892	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	5	6	from	fruit	977:981	arg1	1:1					911:913	1:1	911:913	1:1	911:913	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	5	6	from	fruit	977:981	arg1	mixture					902:908	the mixture	898:908	the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4	898:1000	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	11	7	theme	Elliot	1751:1756	arg1	promising					1785:1793	promising	1785:1793	promising	1785:1793	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	11	7	theme	Elliot	1751:1756	arg1	"					1757:1757	"Elliot"	1750:1757	"Elliot"	1750:1757	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	1	8	theme	cultivars	262:270	arg1	composition					223:233	physical and chemical composition	201:233	physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot")	201:322	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	11	9	theme	product	1854:1860	arg1	terms					1835:1839	terms	1835:1839	terms of the final product stability and bioactives content	1835:1893	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	15	10	theme	high	2529:2532	arg1	level					2534:2538	a high level	2527:2538	a high level of natural bioactive compounds	2527:2569	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	12	11	from	approach	1956:1963	arg1	drying					1978:1983	blueberry drying	1968:1983	blueberry drying	1968:1983	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	15	12	theme	natural	2543:2549	arg1	compounds					2561:2569	natural bioactive compounds	2543:2569	natural bioactive compounds	2543:2569	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	11	13	theme	fruit	1819:1823	arg1	snacks					1825:1830	ready-to-eat fruit snacks	1806:1830	ready-to-eat fruit snacks	1806:1830	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	10	14	theme	drying	1645:1650	arg1	efficiency					1660:1669	the drying process efficiency	1641:1669	the drying process efficiency	1641:1669	With regard to ultrasound applied at the drying step, no explicit effect, either on the drying process efficiency or on final product quality, was identified.					
30199106	15	15	theme	appropriate	2357:2367	arg1	selection					2378:2386	appropriate cultivar selection	2357:2386	appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit	2357:2442	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	1	16	theme	study	175:179	arg1	aim					164:166	The aim	160:166	The aim of the study	160:179	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	13	17	with	intensification	2132:2146	arg1	tissue					2184:2189	dehydrated tissue	2173:2189	dehydrated tissue	2173:2189	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	2	18	theme	cultivars	473:481	arg1	quality					458:464	the nutritional quality	442:464	the nutritional quality of all cultivars	442:481	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	2	18	theme	cultivars	473:481	arg1	drying					414:419	hybrid drying	407:419	hybrid drying on mass transfer	407:436	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	1	19	theme	Elliot	315:320	arg1	"					321:321	"Elliot"	314:321	"Elliot"	314:321	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	3	20	dep	stages	530:535	arg1	initial					542:548	initial	542:548	initial	542:548	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	3	20	dep	stages	530:535	arg1	optimization					551:562	optimization	551:562	two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying)	526:747	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	9	21	theme	final	1483:1487	arg1	effect					1489:1494	The final effect	1479:1494	The final effect	1479:1494	The final effect, however, was strongly influenced by the choice of cultivar.					
30199106	10	22	dep	ultrasound	1572:1581	arg1	regard					1562:1567	regard	1562:1567	regard	1562:1567	With regard to ultrasound applied at the drying step, no explicit effect, either on the drying process efficiency or on final product quality, was identified.					
30199106	3	23	theme	production	662:671	arg1	stages					636:641	all stages	632:641	all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying)	632:747	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	5	24	theme	sucrose	919:925	arg1	solution					927:934	sucrose solution	919:934	sucrose solution	919:934	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	1	25	theme	physical	201:208	arg1	composition					223:233	physical and chemical composition	201:233	physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot")	201:322	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	7	26	theme	dehydration	1391:1401	arg1	stage					1403:1407	the osmotic dehydration stage	1379:1407	the osmotic dehydration stage	1379:1407	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	11	27	theme	ready-to-eat	1806:1817	arg1	snacks					1825:1830	ready-to-eat fruit snacks	1806:1830	ready-to-eat fruit snacks	1806:1830	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	3	28	theme	dried	646:650	arg1	production					662:671	dried blueberry production	646:671	dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying)	646:747	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	1	29	dep	cultivars	262:270	arg1	"					308:308	"	308:308	"	308:308	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	1	29	dep	cultivars	262:270	arg1	"					321:321	"Elliot"	314:321	"Elliot"	314:321	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	1	29	dep	cultivars	262:270	arg1	"					283:283	Bluecrop,"	274:283	"	283:283	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	1	29	dep	cultivars	262:270	arg1	"					296:296	"	296:296	" "Chandler	296:306	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	3	30	theme	osmotic	688:694	arg1	dehydration					696:706	osmotic dehydration	688:706	osmotic dehydration chosen in the initial stage	688:734	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	11	31	theme	same	1723:1726	arg1	conditions					1739:1748	the same processing conditions	1719:1748	the same processing conditions	1719:1748	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	2	32	theme	mass	424:427	arg1	transfer					429:436	mass transfer	424:436	mass transfer	424:436	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	11	33	theme	bioactives	1876:1885	arg1	content					1887:1893	bioactives content	1876:1893	bioactives content	1876:1893	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	7	34	contain	had	1278:1280	arg1	US					1275:1276	US	1275:1276	US	1275:1276	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	7	34	contain	had	1278:1280	arg2	influence					1291:1299	positive influence	1282:1299	positive influence	1282:1299	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	7	35	theme	mechanically	1352:1363	arg1	scratched					1365:1373	mechanically scratched	1352:1373	mechanically scratched	1352:1373	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	4	36	theme	important	843:851	arg1	indices					861:867	the most important quality indices	834:867	the most important quality indices	834:867	In both parts, the effect of US and cultivar were considered as factors influencing the most important quality indices.					
30199106	10	37	theme	product	1683:1689	arg1	quality					1691:1697	final product quality	1677:1697	final product quality	1677:1697	With regard to ultrasound applied at the drying step, no explicit effect, either on the drying process efficiency or on final product quality, was identified.					
30199106	2	38	theme	nutritional	446:456	arg1	quality					458:464	the nutritional quality	442:464	the nutritional quality of all cultivars	442:481	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	15	39	theme	blueberry	2428:2436	arg1	fruit					2438:2442	ready-to-eat dried blueberry fruit	2409:2442	ready-to-eat dried blueberry fruit	2409:2442	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	13	40	theme	dehydrated	2173:2182	arg1	tissue					2184:2189	dehydrated tissue	2173:2189	dehydrated tissue	2173:2189	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	9	41	theme	cultivar	1547:1554	arg1	choice					1537:1542	the choice	1533:1542	the choice of cultivar	1533:1554	The final effect, however, was strongly influenced by the choice of cultivar.					
30199106	1	42	theme	Blue	291:294	arg1	"					283:283	Bluecrop,"	274:283	"	283:283	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	12	43	theme	blueberry	1968:1976	arg1	drying					1978:1983	blueberry drying	1968:1983	blueberry drying	1968:1983	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	5	44	theme	water	1022:1026	arg1	bath					1028:1031	water bath	1022:1031	water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L)	1022:1085	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	3	45	dep	production	662:671	arg1	drying					741:746	drying	741:746	drying	741:746	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	3	45	dep	production	662:671	arg1	pretreatment					674:685	pretreatment	674:685	pretreatment	674:685	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	3	45	dep	production	662:671	arg1	dehydration					696:706	osmotic dehydration	688:706	osmotic dehydration chosen in the initial stage	688:734	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	15	46	theme	process	2468:2474	arg1	parameters					2476:2485	process parameters	2468:2485	process parameters in obtaining an attractive product with a high level of natural bioactive compounds	2468:2569	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	2	47	theme	osmotic	371:377	arg1	step					391:394	the osmotic dehydration step	367:394	the osmotic dehydration step	367:394	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	15	48	theme	attractive	2503:2512	arg1	product					2514:2520	an attractive product	2500:2520	an attractive product	2500:2520	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	7	49	dep	had	1278:1280	arg1	%					1346:1346	12%	1344:1346	12% for mechanically scratched	1344:1373	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	7	49	dep	had	1278:1280	arg1	%					1327:1327	up to 22%	1319:1327	up to 22% for intact	1319:1338	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	2	50	theme	ultrasound	343:352	arg1	influence					354:362	ultrasound influence	343:362	ultrasound influence	343:362	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	6	51	theme	drum	1236:1239	arg1	dryer					1241:1245	hybrid (microwave-ultrasound-convective) drum dryer	1195:1245	hybrid (microwave-ultrasound-convective) drum dryer	1195:1245	After pretreatment osmo-dehydrated fruits were subjected to drying in hybrid (microwave-ultrasound-convective) drum dryer.					
30199106	1	52	theme	Bluecrop	274:281	arg1	"					283:283	Bluecrop,"	274:283	"	283:283	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	1	53	dep	"	296:296	arg1	Chandler					299:306	Chandler	299:306	Chandler	299:306	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	12	54	theme	APPLICATION	1906:1916	arg1	approach					1956:1963	a novel approach	1948:1963	a novel approach in blueberry drying	1948:1983	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	12	54	theme	APPLICATION	1906:1916	arg1	drying					1925:1930	PRACTICAL APPLICATION Hybrid drying	1896:1930	PRACTICAL APPLICATION Hybrid drying	1896:1930	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	6	55	theme	microwave-ultrasound-convective	1203:1233	arg1	dryer					1241:1245	hybrid (microwave-ultrasound-convective) drum dryer	1195:1245	hybrid (microwave-ultrasound-convective) drum dryer	1195:1245	After pretreatment osmo-dehydrated fruits were subjected to drying in hybrid (microwave-ultrasound-convective) drum dryer.					
30199106	0	56	theme	Selection	41:49	arg1	Influence					4:12	The Influence	0:12	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.	0:158	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	13	57	theme	transfer	2123:2130	arg1	intensification					2132:2146	mass transfer intensification	2118:2146	mass transfer intensification	2118:2146	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	3	58	theme	dehydration	575:585	arg1	procedure					587:595	osmotic dehydration procedure	567:595	osmotic dehydration procedure	567:595	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	3	58	theme	dehydration	575:585	arg1	experiment					611:620	the main experiment	602:620	(2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying)	598:747	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	15	59	from	selection	2378:2386	arg1	production					2395:2404	the production	2391:2404	the production of ready-to-eat dried blueberry fruit	2391:2442	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	7	60	theme	mass	1304:1307	arg1	transfer					1309:1316	mass transfer	1304:1316	mass transfer	1304:1316	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	2	61	from	quality	458:464	arg1	transfer					429:436	mass transfer	424:436	mass transfer	424:436	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	10	62	theme	process	1652:1658	arg1	efficiency					1660:1669	the drying process efficiency	1641:1669	the drying process efficiency	1641:1669	With regard to ultrasound applied at the drying step, no explicit effect, either on the drying process efficiency or on final product quality, was identified.					
30199106	15	63	theme	fruit	2438:2442	arg1	production					2395:2404	the production	2391:2404	the production of ready-to-eat dried blueberry fruit	2391:2442	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	8	64	theme	compounds	1460:1468	arg1	content					1470:1476	phenolic compounds content	1451:1476	phenolic compounds content	1451:1476	Unfavorably, its action tend to decrease phenolic compounds content.					
30199106	1	65	theme	blueberry	252:260	arg1	cultivars					262:270	four highbush blueberry cultivars	238:270	four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot")	238:322	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	10	66	from	effect	1623:1628	arg1	quality					1691:1697	final product quality	1677:1697	final product quality	1677:1697	With regard to ultrasound applied at the drying step, no explicit effect, either on the drying process efficiency or on final product quality, was identified.					
30199106	10	66	from	effect	1623:1628	arg1	efficiency					1660:1669	the drying process efficiency	1641:1669	the drying process efficiency	1641:1669	With regard to ultrasound applied at the drying step, no explicit effect, either on the drying process efficiency or on final product quality, was identified.					
30199106	13	67	theme	Ultrasound	1986:1995	arg1	application					1997:2007	Ultrasound application	1986:2007	Ultrasound application as a part of abovementioned drying technique	1986:2052	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	10	68	theme	final	1677:1681	arg1	quality					1691:1697	final product quality	1677:1697	final product quality	1677:1697	With regard to ultrasound applied at the drying step, no explicit effect, either on the drying process efficiency or on final product quality, was identified.					
30199106	11	69	theme	processing	1728:1737	arg1	conditions					1739:1748	the same processing conditions	1719:1748	the same processing conditions	1719:1748	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	6	70	theme	hybrid	1195:1200	arg1	dryer					1241:1245	hybrid (microwave-ultrasound-convective) drum dryer	1195:1245	hybrid (microwave-ultrasound-convective) drum dryer	1195:1245	After pretreatment osmo-dehydrated fruits were subjected to drying in hybrid (microwave-ultrasound-convective) drum dryer.					
30199106	6	71	theme	pretreatment	1131:1142	arg1	fruits					1160:1165	pretreatment osmo-dehydrated fruits	1131:1165	pretreatment osmo-dehydrated fruits	1131:1165	After pretreatment osmo-dehydrated fruits were subjected to drying in hybrid (microwave-ultrasound-convective) drum dryer.					
30199106	13	72	theme	efficient	2071:2079	arg1	way					2081:2083	an efficient way	2068:2083	an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue	2068:2189	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	13	73	with	interaction	2156:2166	arg1	tissue					2184:2189	dehydrated tissue	2173:2189	dehydrated tissue	2173:2189	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	11	74	theme	final	1848:1852	arg1	product					1854:1860	the final product stability and bioactives content	1844:1893	product	1854:1860	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	5	75	dep	transducers	1056:1066	arg1	25 kHz					1069:1074	25 kHz	1069:1074	25 kHz	1069:1074	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	5	75	dep	transducers	1056:1066	arg1	27.8 W/L					1077:1084	27.8 W/L	1077:1084	27.8 W/L	1077:1084	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	15	76	theme	cultivar	2369:2376	arg1	selection					2378:2386	appropriate cultivar selection	2357:2386	appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit	2357:2442	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	13	77	theme	abovementioned	2022:2035	arg1	technique					2044:2052	abovementioned drying technique	2022:2052	abovementioned drying technique	2022:2052	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	2	78	from	drying	414:419	arg1	transfer					429:436	mass transfer	424:436	mass transfer	424:436	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	5	79	from	dehydration	882:892	arg1	1:1					911:913	1:1	911:913	1:1	911:913	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	5	79	from	dehydration	882:892	arg1	mixture					902:908	the mixture	898:908	the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4	898:1000	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	5	79	from	dehydration	882:892	arg1	fruit					977:981	a fruit	975:981	a fruit to syrup ratio 1:4	975:1000	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	13	80	theme	technique	2044:2052	arg1	part					2014:2017	a part	2012:2017	a part of abovementioned drying technique	2012:2052	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	7	81	theme	osmotic	1383:1389	arg1	stage					1403:1407	the osmotic dehydration stage	1379:1407	the osmotic dehydration stage	1379:1407	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	3	82	dep	experiment	611:620	arg1	2					599:599	2	599:599	2	599:599	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	11	83	dep	product	1854:1860	arg1	stability					1862:1870	stability	1862:1870	stability	1862:1870	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	0	84	theme	Snacks	152:157	arg1	Biocompounds					58:69	Biocompounds	58:69	Biocompounds	58:69	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	0	84	theme	Snacks	152:157	arg1	Characteristics					91:105	Physicochemical Characteristics	75:105	Physicochemical Characteristics	75:105	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	5	85	theme	solution	927:934	arg1	1:1					911:913	1:1	911:913	1:1	911:913	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	5	85	theme	solution	927:934	arg1	mixture					902:908	the mixture	898:908	the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4	898:1000	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	4	86	theme	US	779:780	arg1	factors					814:820	factors	814:820	factors influencing the most important quality indices	814:867	In both parts, the effect of US and cultivar were considered as factors influencing the most important quality indices.					
30199106	4	86	theme	US	779:780	arg1	effect					769:774	the effect	765:774	the effect of US and cultivar	765:793	In both parts, the effect of US and cultivar were considered as factors influencing the most important quality indices.					
30199106	3	87	theme	blueberry	652:660	arg1	production					662:671	dried blueberry production	646:671	dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying)	646:747	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	15	88	theme	compounds	2561:2569	arg1	level					2534:2538	a high level	2527:2538	a high level of natural bioactive compounds	2527:2569	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	10	89	theme	drying	1598:1603	arg1	step					1605:1608	the drying step	1594:1608	the drying step	1594:1608	With regard to ultrasound applied at the drying step, no explicit effect, either on the drying process efficiency or on final product quality, was identified.					
30199106	0	90	theme	Physicochemical	75:89	arg1	Characteristics					91:105	Physicochemical Characteristics	75:105	Physicochemical Characteristics	75:105	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	0	91	dep	Vaccinium	127:135	arg1	L.					148:149	Vaccinium corymbosum L.	127:149	Vaccinium corymbosum L.	127:149	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	2	92	theme	hybrid	407:412	arg1	drying					414:419	hybrid drying	407:419	hybrid drying on mass transfer	407:436	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	0	93	from	Influence	4:12	arg1	Biocompounds					58:69	Biocompounds	58:69	Biocompounds	58:69	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	0	93	from	Influence	4:12	arg1	Characteristics					91:105	Physicochemical Characteristics	75:105	Physicochemical Characteristics	75:105	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	3	94	theme	initial	722:728	arg1	stage					730:734	the initial stage	718:734	the initial stage	718:734	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	15	95	theme	dried	2422:2426	arg1	fruit					2438:2442	ready-to-eat dried blueberry fruit	2409:2442	ready-to-eat dried blueberry fruit	2409:2442	This study contributes to the understanding that appropriate cultivar selection in the production of ready-to-eat dried blueberry fruit is just as important as process parameters in obtaining an attractive product with a high level of natural bioactive compounds.					
30199106	6	96	from	drying	1185:1190	arg1	dryer					1241:1245	hybrid (microwave-ultrasound-convective) drum dryer	1195:1245	hybrid (microwave-ultrasound-convective) drum dryer	1195:1245	After pretreatment osmo-dehydrated fruits were subjected to drying in hybrid (microwave-ultrasound-convective) drum dryer.					
30199106	2	97	from	step	391:394	arg1	assessment					329:338	The assessment	325:338	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars	325:481	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	14	98	from	impact	2229:2234	arg1	cultivars					2261:2269	various cultivars	2253:2269	various cultivars	2253:2269	However, no experiments studying the impact of ultrasound on various cultivars within one species were carried out.					
30199106	12	99	theme	novel	1950:1954	arg1	approach					1956:1963	a novel approach	1948:1963	a novel approach in blueberry drying	1948:1983	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	12	99	theme	novel	1950:1954	arg1	drying					1925:1930	PRACTICAL APPLICATION Hybrid drying	1896:1930	PRACTICAL APPLICATION Hybrid drying	1896:1930	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	0	100	theme	Blueberry	116:124	arg1	Snacks					152:157	Dried Blueberry (Vaccinium corymbosum L.) Snacks	110:157	Dried Blueberry (Vaccinium corymbosum L.) Snacks	110:157	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	5	101	theme	apple	940:944	arg1	65°Bx					965:969	65°Bx	965:969	65°Bx	965:969	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	5	101	theme	apple	940:944	arg1	concentrate					952:962	apple juice concentrate	940:962	apple juice concentrate (65°Bx)	940:970	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	13	102	theme	drying	2037:2042	arg1	technique					2044:2052	abovementioned drying technique	2022:2052	abovementioned drying technique	2022:2052	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	1	103	theme	chemical	214:221	arg1	composition					223:233	physical and chemical composition	201:233	physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot")	201:322	The aim of the study was to characterize physical and chemical composition of four highbush blueberry cultivars ("Bluecrop," "Late Blue," "Chandler," and "Elliot").					
30199106	6	104	theme	osmo-dehydrated	1144:1158	arg1	fruits					1160:1165	pretreatment osmo-dehydrated fruits	1131:1165	pretreatment osmo-dehydrated fruits	1131:1165	After pretreatment osmo-dehydrated fruits were subjected to drying in hybrid (microwave-ultrasound-convective) drum dryer.					
30199106	5	105	theme	concentrate	952:962	arg1	1:1					911:913	1:1	911:913	1:1	911:913	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	5	105	theme	concentrate	952:962	arg1	mixture					902:908	the mixture	898:908	the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4	898:1000	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	4	106	theme	cultivar	786:793	arg1	factors					814:820	factors	814:820	factors influencing the most important quality indices	814:867	In both parts, the effect of US and cultivar were considered as factors influencing the most important quality indices.					
30199106	4	106	theme	cultivar	786:793	arg1	effect					769:774	the effect	765:774	the effect of US and cultivar	765:793	In both parts, the effect of US and cultivar were considered as factors influencing the most important quality indices.					
30199106	0	107	theme	Ultrasound	17:26	arg1	Influence					4:12	The Influence	0:12	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.	0:158	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	0	108	dep	Biocompounds	58:69	arg1	the					54:56	the	54:56	the	54:56	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	2	109	theme	influence	354:362	arg1	assessment					329:338	The assessment	325:338	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars	325:481	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	10	110	theme	explicit	1614:1621	arg1	effect					1623:1628	no explicit effect	1611:1628	no explicit effect	1611:1628	With regard to ultrasound applied at the drying step, no explicit effect, either on the drying process efficiency or on final product quality, was identified.					
30199106	5	111	theme	40°C	1107:1110	arg1	temperature					1092:1102	a temperature	1090:1102	a temperature of 40°C for 150 min	1090:1122	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	0	112	theme	Cultivar	32:39	arg1	Selection					41:49	Cultivar Selection	32:49	Cultivar Selection	32:49	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	5	113	theme	osmotic	874:880	arg1	dehydration					882:892	The osmotic dehydration	870:892	The osmotic dehydration	870:892	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	4	114	theme	quality	853:859	arg1	indices					861:867	the most important quality indices	834:867	the most important quality indices	834:867	In both parts, the effect of US and cultivar were considered as factors influencing the most important quality indices.					
30199106	12	115	theme	PRACTICAL	1896:1904	arg1	approach					1956:1963	a novel approach	1948:1963	a novel approach in blueberry drying	1948:1983	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	12	115	theme	PRACTICAL	1896:1904	arg1	drying					1925:1930	PRACTICAL APPLICATION Hybrid drying	1896:1930	PRACTICAL APPLICATION Hybrid drying	1896:1930	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	0	116	theme	Dried	110:114	arg1	Snacks					152:157	Dried Blueberry (Vaccinium corymbosum L.) Snacks	110:157	Dried Blueberry (Vaccinium corymbosum L.) Snacks	110:157	The Influence of Ultrasound and Cultivar Selection on the Biocompounds and Physicochemical Characteristics of Dried Blueberry (Vaccinium corymbosum L.) Snacks.					
30199106	13	117	theme	mass	2118:2121	arg1	intensification					2132:2146	mass transfer intensification	2118:2146	mass transfer intensification	2118:2146	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	5	118	theme	juice	946:950	arg1	65°Bx					965:969	65°Bx	965:969	65°Bx	965:969	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	5	118	theme	juice	946:950	arg1	concentrate					952:962	apple juice concentrate	940:962	apple juice concentrate (65°Bx)	940:970	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	2	119	theme	dehydration	379:389	arg1	step					391:394	the osmotic dehydration step	367:394	the osmotic dehydration step	367:394	The assessment of ultrasound influence at the osmotic dehydration step and during hybrid drying on mass transfer and the nutritional quality of all cultivars was also done.					
30199106	3	120	theme	osmotic	567:573	arg1	procedure					587:595	osmotic dehydration procedure	567:595	osmotic dehydration procedure	567:595	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	3	120	theme	osmotic	567:573	arg1	experiment					611:620	the main experiment	602:620	(2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying)	598:747	The experiment consisted of two stages: (1) initial: optimization of osmotic dehydration procedure, (2) the main experiment including all stages of dried blueberry production (pretreatment, osmotic dehydration chosen in the initial stage, and drying).					
30199106	12	121	theme	Hybrid	1918:1923	arg1	approach					1956:1963	a novel approach	1948:1963	a novel approach in blueberry drying	1948:1983	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	12	121	theme	Hybrid	1918:1923	arg1	drying					1925:1930	PRACTICAL APPLICATION Hybrid drying	1896:1930	PRACTICAL APPLICATION Hybrid drying	1896:1930	PRACTICAL APPLICATION Hybrid drying was proposed as a novel approach in blueberry drying.					
30199106	5	122	theme	syrup	986:990	arg1	ratio					992:996	syrup ratio 1:4	986:1000	syrup ratio 1:4	986:1000	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
30199106	14	123	theme	various	2253:2259	arg1	cultivars					2261:2269	various cultivars	2253:2269	various cultivars	2253:2269	However, no experiments studying the impact of ultrasound on various cultivars within one species were carried out.					
30199106	7	124	dep	22	1325:1326	arg1	to					1322:1323	to	1322:1323	to	1322:1323	Regardless of the cultivar US had positive influence on mass transfer (up to 22% for intact and 12% for mechanically scratched) at the osmotic dehydration stage.					
30199106	14	125	theme	ultrasound	2239:2248	arg1	impact					2229:2234	the impact	2225:2234	the impact of ultrasound on various cultivars	2225:2269	However, no experiments studying the impact of ultrasound on various cultivars within one species were carried out.					
30199106	13	126	theme	drying	2099:2104	arg1	time					2106:2109	drying time	2099:2109	drying time	2099:2109	Ultrasound application as a part of abovementioned drying technique is considered an efficient way of shortening drying time due to mass transfer intensification and the interaction with dehydrated tissue.					
30199106	11	127	theme	content	1887:1893	arg1	terms					1835:1839	terms	1835:1839	terms of the final product stability and bioactives content	1835:1893	In the same processing conditions "Elliot" turned out to be the most promising to produce ready-to-eat fruit snacks in terms of the final product stability and bioactives content.					
30199106	8	128	theme	phenolic	1451:1458	arg1	content					1470:1476	phenolic compounds content	1451:1476	phenolic compounds content	1451:1476	Unfavorably, its action tend to decrease phenolic compounds content.					
30199106	5	129	theme	ultrasonic	1045:1054	arg1	transducers					1056:1066	ultrasonic transducers	1045:1066	ultrasonic transducers (25 kHz, 27.8 W/L)	1045:1085	The osmotic dehydration, in the mixture (1:1) of sucrose solution and apple juice concentrate (65°Bx) at a fruit to syrup ratio 1:4, was carried out in water bath fitted with ultrasonic transducers (25 kHz, 27.8 W/L) at a temperature of 40°C for 150 min.					
31322980	2	0	with	cytocompatibility	406:422	arg1	cells					452:456	human embryonic kidney cells	429:456	human embryonic kidney cells	429:456	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	1	1	from	applications	190:201	arg1	construction					210:221	the construction	206:221	the construction of eco-friendly biomedical products	206:257	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	2	2	theme	nanocomposite	384:396	arg1	characteristics					323:337	the chemical-physical characteristics	301:337	the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells	301:456	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	6	3	theme	improved	886:893	arg1	spreading					900:908	improved cell spreading	886:908	improved cell spreading	886:908	Cell proliferation and changes in cell morphology indicated that the nanocomposite led to improved cell spreading and growth.					
31322980	1	4	theme	attractive	175:184	arg1	Cellulose					105:113	Cellulose	105:113	Cellulose	105:113	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	1	4	theme	attractive	175:184	arg1	polymer					130:136	a renewable polymer	118:136	a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products	118:257	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	3	5	theme	swelling	492:499	arg1	ratio					501:505	swelling ratio	492:505	swelling ratio	492:505	First, the chemical composition, swelling ratio and surface topography of the nanocomposite were evaluated.					
31322980	5	6	theme	effective	712:720	arg1	agent					735:739	an effective nanomaterial agent	709:739	an effective nanomaterial agent for increasing the roughness surface of chitosan film	709:793	The experimental results showed that the CCN was an effective nanomaterial agent for increasing the roughness surface of chitosan film.					
31322980	5	6	theme	effective	712:720	arg1	CCN					701:703	the CCN	697:703	the CCN	697:703	The experimental results showed that the CCN was an effective nanomaterial agent for increasing the roughness surface of chitosan film.					
31322980	2	7	theme	chemical-physical	305:321	arg1	characteristics					323:337	the chemical-physical characteristics	301:337	the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells	301:456	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	4	8	theme	cells	653:657	arg1	spreading					611:619	spreading	611:619	spreading	611:619	Cytocompatibility was then assessed through spreading, proliferation and viability of cells.					
31322980	4	8	theme	cells	653:657	arg1	viability					640:648	viability	640:648	viability	640:648	Cytocompatibility was then assessed through spreading, proliferation and viability of cells.					
31322980	4	8	theme	cells	653:657	arg1	proliferation					622:634	proliferation	622:634	proliferation	622:634	Cytocompatibility was then assessed through spreading, proliferation and viability of cells.					
31322980	8	9	theme	biomedical	1140:1149	arg1	applications					1151:1162	biomedical applications	1140:1162	biomedical applications	1140:1162	The results indicate that CCN/chitosan nanocomposite could be a promising biocompatible biomaterial for biomedical applications.					
31322980	5	10	theme	nanomaterial	722:733	arg1	agent					735:739	an effective nanomaterial agent	709:739	an effective nanomaterial agent for increasing the roughness surface of chitosan film	709:793	The experimental results showed that the CCN was an effective nanomaterial agent for increasing the roughness surface of chitosan film.					
31322980	5	10	theme	nanomaterial	722:733	arg1	CCN					701:703	the CCN	697:703	the CCN	697:703	The experimental results showed that the CCN was an effective nanomaterial agent for increasing the roughness surface of chitosan film.					
31322980	5	11	theme	experimental	664:675	arg1	results					677:683	The experimental results	660:683	The experimental results	660:683	The experimental results showed that the CCN was an effective nanomaterial agent for increasing the roughness surface of chitosan film.					
31322980	3	12	theme	nanocomposite	537:549	arg1	composition					479:489	the chemical composition	466:489	the chemical composition	466:489	First, the chemical composition, swelling ratio and surface topography of the nanocomposite were evaluated.					
31322980	3	12	theme	nanocomposite	537:549	arg1	ratio					501:505	swelling ratio	492:505	swelling ratio	492:505	First, the chemical composition, swelling ratio and surface topography of the nanocomposite were evaluated.					
31322980	3	12	theme	nanocomposite	537:549	arg1	topography					519:528	surface topography	511:528	surface topography	511:528	First, the chemical composition, swelling ratio and surface topography of the nanocomposite were evaluated.					
31322980	8	13	theme	biocompatible	1110:1122	arg1	nanocomposite					1075:1087	CCN/chitosan nanocomposite	1062:1087	CCN/chitosan nanocomposite	1062:1087	The results indicate that CCN/chitosan nanocomposite could be a promising biocompatible biomaterial for biomedical applications.					
31322980	8	13	theme	biocompatible	1110:1122	arg1	biomaterial					1124:1134	a promising biocompatible biomaterial	1098:1134	a promising biocompatible biomaterial for biomedical applications	1098:1162	The results indicate that CCN/chitosan nanocomposite could be a promising biocompatible biomaterial for biomedical applications.					
31322980	7	14	from	survival	984:991	arg1	nanocomposite					1000:1012	the nanocomposite	996:1012	the nanocomposite	996:1012	Cell viability did not decrease after 24 h. However, the cell survival on the nanocomposite was affected at 72 h.					
31322980	2	15	theme	embryonic	435:443	arg1	cells					452:456	human embryonic kidney cells	429:456	human embryonic kidney cells	429:456	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	8	16	theme	CCN/chitosan	1062:1073	arg1	nanocomposite					1075:1087	CCN/chitosan nanocomposite	1062:1087	CCN/chitosan nanocomposite	1062:1087	The results indicate that CCN/chitosan nanocomposite could be a promising biocompatible biomaterial for biomedical applications.					
31322980	8	16	theme	CCN/chitosan	1062:1073	arg1	biomaterial					1124:1134	a promising biocompatible biomaterial	1098:1134	a promising biocompatible biomaterial for biomedical applications	1098:1162	The results indicate that CCN/chitosan nanocomposite could be a promising biocompatible biomaterial for biomedical applications.					
31322980	2	17	theme	human	429:433	arg1	cells					452:456	human embryonic kidney cells	429:456	human embryonic kidney cells	429:456	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	0	18	theme	cellulose	7:15	arg1	nanofiber/chitosan					17:34	Cotton cellulose nanofiber/chitosan	0:34	Cotton cellulose nanofiber/chitosan	0:34	Cotton cellulose nanofiber/chitosan nanocomposite: characterization and evaluation of cytocompatibility.					
31322980	8	19	theme	promising	1100:1108	arg1	nanocomposite					1075:1087	CCN/chitosan nanocomposite	1062:1087	CCN/chitosan nanocomposite	1062:1087	The results indicate that CCN/chitosan nanocomposite could be a promising biocompatible biomaterial for biomedical applications.					
31322980	8	19	theme	promising	1100:1108	arg1	biomaterial					1124:1134	a promising biocompatible biomaterial	1098:1134	a promising biocompatible biomaterial for biomedical applications	1098:1162	The results indicate that CCN/chitosan nanocomposite could be a promising biocompatible biomaterial for biomedical applications.					
31322980	2	20	theme	cellulose	349:357	arg1	nanocomposite					384:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	7	21	theme	Cell	922:925	arg1	viability					927:935	Cell viability	922:935	Cell viability	922:935	Cell viability did not decrease after 24 h. However, the cell survival on the nanocomposite was affected at 72 h.					
31322980	0	22	theme	Cotton	0:5	arg1	nanofiber/chitosan					17:34	Cotton cellulose nanofiber/chitosan	0:34	Cotton cellulose nanofiber/chitosan	0:34	Cotton cellulose nanofiber/chitosan nanocomposite: characterization and evaluation of cytocompatibility.					
31322980	6	23	theme	cell	895:898	arg1	spreading					900:908	improved cell spreading	886:908	improved cell spreading	886:908	Cell proliferation and changes in cell morphology indicated that the nanocomposite led to improved cell spreading and growth.					
31322980	2	24	theme	cotton	342:347	arg1	nanocomposite					384:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	2	25	theme	cytocompatibility	406:422	arg1	characteristics					323:337	the chemical-physical characteristics	301:337	the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells	301:456	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	5	26	theme	roughness	760:768	arg1	surface					770:776	the roughness surface	756:776	the roughness surface of chitosan film	756:793	The experimental results showed that the CCN was an effective nanomaterial agent for increasing the roughness surface of chitosan film.					
31322980	1	27	theme	renewable	120:128	arg1	Cellulose					105:113	Cellulose	105:113	Cellulose	105:113	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	1	27	theme	renewable	120:128	arg1	polymer					130:136	a renewable polymer	118:136	a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products	118:257	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	2	28	theme	/chitosan	374:382	arg1	nanocomposite					384:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	2	29	theme	CCN	370:372	arg1	nanocomposite					384:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	1	30	theme	eco-friendly	226:237	arg1	products					250:257	eco-friendly biomedical products	226:257	eco-friendly biomedical products	226:257	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	3	31	theme	chemical	470:477	arg1	composition					479:489	the chemical composition	466:489	the chemical composition	466:489	First, the chemical composition, swelling ratio and surface topography of the nanocomposite were evaluated.					
31322980	6	32	from	proliferation	801:813	arg1	morphology					835:844	cell morphology	830:844	cell morphology	830:844	Cell proliferation and changes in cell morphology indicated that the nanocomposite led to improved cell spreading and growth.					
31322980	5	33	theme	chitosan	781:788	arg1	surface					770:776	the roughness surface	756:776	the roughness surface of chitosan film	756:793	The experimental results showed that the CCN was an effective nanomaterial agent for increasing the roughness surface of chitosan film.					
31322980	6	34	theme	cell	830:833	arg1	morphology					835:844	cell morphology	830:844	cell morphology	830:844	Cell proliferation and changes in cell morphology indicated that the nanocomposite led to improved cell spreading and growth.					
31322980	1	35	theme	biomedical	239:248	arg1	products					250:257	eco-friendly biomedical products	226:257	eco-friendly biomedical products	226:257	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	6	36	from	changes	819:825	arg1	morphology					835:844	cell morphology	830:844	cell morphology	830:844	Cell proliferation and changes in cell morphology indicated that the nanocomposite led to improved cell spreading and growth.					
31322980	2	37	with	nanocomposite	384:396	arg1	cells					452:456	human embryonic kidney cells	429:456	human embryonic kidney cells	429:456	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	1	38	theme	abundant	144:151	arg1	Cellulose					105:113	Cellulose	105:113	Cellulose	105:113	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	1	38	theme	abundant	144:151	arg1	polymer					130:136	a renewable polymer	118:136	a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products	118:257	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	3	39	theme	surface	511:517	arg1	topography					519:528	surface topography	511:528	surface topography	511:528	First, the chemical composition, swelling ratio and surface topography of the nanocomposite were evaluated.					
31322980	7	40	theme	cell	979:982	arg1	survival					984:991	the cell survival	975:991	the cell survival on the nanocomposite	975:1012	Cell viability did not decrease after 24 h. However, the cell survival on the nanocomposite was affected at 72 h.					
31322980	1	41	theme	products	250:257	arg1	construction					210:221	the construction	206:221	the construction of eco-friendly biomedical products	206:257	Cellulose is a renewable polymer quite abundant on the Earth and very attractive for applications in the construction of eco-friendly biomedical products.					
31322980	2	42	theme	study	276:280	arg1	aim					264:266	The aim	260:266	The aim of this study	260:280	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	2	43	theme	nanofiber	359:367	arg1	nanocomposite					384:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	cotton cellulose nanofiber (CCN)/chitosan nanocomposite	342:396	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	0	44	theme	cytocompatibility	86:102	arg1	characterization					51:66	characterization	51:66	characterization	51:66	Cotton cellulose nanofiber/chitosan nanocomposite: characterization and evaluation of cytocompatibility.					
31322980	0	44	theme	cytocompatibility	86:102	arg1	evaluation					72:81	evaluation	72:81	evaluation	72:81	Cotton cellulose nanofiber/chitosan nanocomposite: characterization and evaluation of cytocompatibility.					
31322980	2	45	theme	kidney	445:450	arg1	cells					452:456	human embryonic kidney cells	429:456	human embryonic kidney cells	429:456	The aim of this study was to investigate the chemical-physical characteristics of cotton cellulose nanofiber (CCN)/chitosan nanocomposite and its cytocompatibility with human embryonic kidney cells.					
31322980	6	46	theme	Cell	796:799	arg1	proliferation					801:813	Cell proliferation	796:813	Cell proliferation	796:813	Cell proliferation and changes in cell morphology indicated that the nanocomposite led to improved cell spreading and growth.					
30425148	8	0	theme	various	1720:1726	arg1	processes					1728:1736	various processes	1720:1736	various processes crucial for growth, motility, and virulence	1720:1780	Here, we characterized the biological function of the sRNA PmaR that controls various processes crucial for growth, motility, and virulence.					
30425148	8	1	theme	biological	1669:1678	arg1	function					1680:1687	the biological function	1665:1687	the biological function of the sRNA PmaR that controls various processes crucial for growth, motility, and virulence	1665:1780	Here, we characterized the biological function of the sRNA PmaR that controls various processes crucial for growth, motility, and virulence.					
30425148	3	2	theme	sRNA	528:531	arg1	PmaR					533:536	the sRNA PmaR	524:536	the sRNA PmaR for peptidoglycan biosynthesis, motility, and ampicillin resistance regulator	524:614	According to these targets, we named the sRNA PmaR for peptidoglycan biosynthesis, motility, and ampicillin resistance regulator.					
30425148	8	3	theme	crucial	1738:1744	arg1	processes					1728:1736	various processes	1720:1736	various processes crucial for growth, motility, and virulence	1720:1780	Here, we characterized the biological function of the sRNA PmaR that controls various processes crucial for growth, motility, and virulence.					
30425148	5	4	theme	resistance	1198:1207	arg1	gene.IMPORTANCE					1209:1223	an antibiotic resistance gene.IMPORTANCE	1184:1223	an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	1184:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	5	dep	regulation	780:789	arg1	revealed					841:848	revealed	841:848	revealed	841:848	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	8	6	theme	PmaR	1701:1704	arg1	function					1680:1687	the biological function	1665:1687	the biological function of the sRNA PmaR that controls various processes crucial for growth, motility, and virulence	1665:1780	Here, we characterized the biological function of the sRNA PmaR that controls various processes crucial for growth, motility, and virulence.					
30425148	2	7	theme	plant	289:293	arg1	tumefaciens					318:328	the plant pathogen Agrobacterium tumefaciens	285:328	the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics	285:404	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	5	8	dep	Agrobacterium	1250:1262	arg1	tumefaciens					1264:1274	tumefaciens	1264:1274	tumefaciens	1264:1274	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	9	9	theme	microbial	1964:1972	arg1	composition					1974:1984	the competitive microbial composition	1948:1984	the competitive microbial composition of the rhizosphere	1948:2003	Among the genes directly targeted by PmaR is ampC coding for a beta-lactamase that confers ampicillin resistance, suggesting that the sRNA is crucial for fitness in the competitive microbial composition of the rhizosphere.					
30425148	7	10	dep	A.	1615:1616	arg1	tumefaciens					1618:1628	tumefaciens	1618:1628	tumefaciens	1618:1628	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	2	11	theme	regulatory	220:229	arg1	sRNA					236:239	sRNA	236:239	sRNA	236:239	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	2	11	theme	regulatory	220:229	arg1	RNA					231:233	a differentially expressed small regulatory RNA	187:233	a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA	187:484	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	7	12	from	presence	1507:1514	arg1	organism					1554:1561	this organism	1549:1561	this organism	1549:1561	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	5	13	theme	resistance	1031:1040	arg1	uptake					1062:1067	uptake	1062:1067	uptake	1062:1067	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	13	theme	resistance	1031:1040	arg1	transporters					1080:1091	efflux transporters	1073:1091	efflux transporters	1073:1091	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	13	theme	resistance	1031:1040	arg1	mechanisms					1042:1051	antibiotic resistance mechanisms	1020:1051	antibiotic resistance mechanisms	1020:1051	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	2	14	theme	expressed	204:212	arg1	sRNA					236:239	sRNA	236:239	sRNA	236:239	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	2	14	theme	expressed	204:212	arg1	RNA					231:233	a differentially expressed small regulatory RNA	187:233	a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA	187:484	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	1	15	theme	important	112:120	arg1	role					122:125	an important role	109:125	an important role	109:125	Small regulatory RNAs play an important role in the adaptation to changing conditions.					
30425148	9	16	theme	rhizosphere	1993:2003	arg1	composition					1974:1984	the competitive microbial composition	1948:1984	the competitive microbial composition of the rhizosphere	1948:2003	Among the genes directly targeted by PmaR is ampC coding for a beta-lactamase that confers ampicillin resistance, suggesting that the sRNA is crucial for fitness in the competitive microbial composition of the rhizosphere.					
30425148	5	17	dep	in	952:953	arg1	vivo					955:958	vivo	955:958	vivo	955:958	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	18	theme	various	1294:1300	arg1	eudicots					1302:1309	various eudicots	1294:1309	various eudicots causing crown gall tumor formation	1294:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	19	theme	high	680:683	arg1	concentrations					696:709	high ampicillin concentrations	680:709	high ampicillin concentrations	680:709	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	2	20	from	processes	272:280	arg1	tumefaciens					318:328	the plant pathogen Agrobacterium tumefaciens	285:328	the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics	285:404	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	3	21	theme	resistance	595:604	arg1	regulator					606:614	ampicillin resistance regulator	584:614	ampicillin resistance regulator	584:614	According to these targets, we named the sRNA PmaR for peptidoglycan biosynthesis, motility, and ampicillin resistance regulator.					
30425148	6	22	theme	gene	1391:1394	arg1	transfer					1396:1403	interkingdom gene transfer	1378:1403	interkingdom gene transfer	1378:1403	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	5	23	theme	first	1118:1122	arg1	example					1124:1130	the first example	1114:1130	the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	1114:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	1	24	theme	changing	148:155	arg1	conditions					157:166	changing conditions	148:166	changing conditions	148:166	Small regulatory RNAs play an important role in the adaptation to changing conditions.					
30425148	2	25	theme	cellular	263:270	arg1	processes					272:280	various cellular processes	255:280	various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics	255:404	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	7	26	theme	considerable	1574:1585	arg1	impact					1587:1592	a considerable impact	1572:1592	a considerable impact of riboregulation on A. tumefaciens physiology	1572:1639	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	6	27	theme	unique	1360:1365	arg1	ability					1367:1373	its unique ability	1356:1373	its unique ability of interkingdom gene transfer	1356:1403	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	6	28	theme	crucial	1432:1438	arg1	tool					1457:1460	a crucial biotechnological tool	1430:1460	a crucial biotechnological tool for genetic manipulation of plant cells	1430:1500	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	6	28	theme	crucial	1432:1438	arg1	Agrobacterium					1406:1418	Agrobacterium	1406:1418	Agrobacterium	1406:1418	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	5	29	theme	in	964:965	arg1	riboregulators					981:994	in vivo and in vitro Several riboregulators	952:994	in vivo and in vitro Several riboregulators	952:994	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	2	30	theme	comparative	383:393	arg1	proteomics					395:404	comparative proteomics	383:404	comparative proteomics	383:404	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	5	31	theme	beta-lactamase	798:811	arg1	gene					813:816	the beta-lactamase gene	794:816	the beta-lactamase gene	794:816	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	1	32	theme	regulatory	88:97	arg1	RNAs					99:102	Small regulatory RNAs	82:102	Small regulatory RNAs	82:102	Small regulatory RNAs play an important role in the adaptation to changing conditions.					
30425148	5	33	theme	gall	1325:1328	arg1	formation					1336:1344	crown gall tumor formation	1319:1344	crown gall tumor formation	1319:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	6	34	theme	biotechnological	1440:1455	arg1	tool					1457:1460	a crucial biotechnological tool	1430:1460	a crucial biotechnological tool for genetic manipulation of plant cells	1430:1500	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	6	34	theme	biotechnological	1440:1455	arg1	Agrobacterium					1406:1418	Agrobacterium	1406:1418	Agrobacterium	1406:1418	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	5	35	theme	PmaR-mediated	766:778	arg1	regulation					780:789	the positive PmaR-mediated regulation	753:789	the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	753:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	0	36	theme	Wall	37:40	arg1	Biosynthesis					42:53	Cell Wall Biosynthesis	32:53	Cell Wall Biosynthesis	32:53	A Small Regulatory RNA Controls Cell Wall Biosynthesis and Antibiotic Resistance.					
30425148	5	37	theme	sRNA	1138:1141	arg1	example					1124:1130	the first example	1114:1130	the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	1114:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	7	38	theme	putative	1531:1538	arg1	sRNAs					1540:1544	putative sRNAs	1531:1544	putative sRNAs	1531:1544	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	5	39	theme	antibiotic	1020:1029	arg1	uptake					1062:1067	uptake	1062:1067	uptake	1062:1067	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	39	theme	antibiotic	1020:1029	arg1	transporters					1080:1091	efflux transporters	1073:1091	efflux transporters	1073:1091	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	39	theme	antibiotic	1020:1029	arg1	mechanisms					1042:1051	antibiotic resistance mechanisms	1020:1051	antibiotic resistance mechanisms	1020:1051	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	40	theme	gene.IMPORTANCE	1209:1223	arg1	expression					1170:1179	the expression	1166:1179	the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	1166:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	7	41	theme	hundreds	1519:1526	arg1	presence					1507:1514	The presence	1503:1514	The presence of hundreds of putative sRNAs in this organism	1503:1561	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	5	42	theme	Several	973:979	arg1	riboregulators					981:994	in vivo and in vitro Several riboregulators	952:994	in vivo and in vitro Several riboregulators	952:994	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	2	43	theme	predictions	367:377	arg1	combination					338:348	a combination	336:348	a combination of bioinformatic predictions and comparative proteomics	336:404	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	6	44	theme	plant	1490:1494	arg1	cells					1496:1500	plant cells	1490:1500	plant cells	1490:1500	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	5	45	theme	antibiotic	1187:1196	arg1	gene.IMPORTANCE					1209:1223	an antibiotic resistance gene.IMPORTANCE	1184:1223	an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	1184:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	3	46	theme	peptidoglycan	542:554	arg1	biosynthesis					556:567	peptidoglycan biosynthesis	542:567	peptidoglycan biosynthesis	542:567	According to these targets, we named the sRNA PmaR for peptidoglycan biosynthesis, motility, and ampicillin resistance regulator.					
30425148	2	47	theme	various	255:261	arg1	processes					272:280	various cellular processes	255:280	various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics	255:404	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	5	48	theme	spoon-like	852:861	arg1	structure					863:871	a spoon-like structure	850:871	a spoon-like structure of the sRNA	850:883	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	2	49	theme	pathogen	295:302	arg1	tumefaciens					318:328	the plant pathogen Agrobacterium tumefaciens	285:328	the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics	285:404	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	5	50	theme	ampicillin	685:694	arg1	concentrations					696:709	high ampicillin concentrations	680:709	high ampicillin concentrations	680:709	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	51	theme	Structure	823:831	arg1	probing					833:839	ampC Structure probing	818:839	ampC Structure probing	818:839	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	9	52	theme	competitive	1952:1962	arg1	composition					1974:1984	the competitive microbial composition	1948:1984	the competitive microbial composition of the rhizosphere	1948:2003	Among the genes directly targeted by PmaR is ampC coding for a beta-lactamase that confers ampicillin resistance, suggesting that the sRNA is crucial for fitness in the competitive microbial composition of the rhizosphere.					
30425148	5	53	dep	gene.IMPORTANCE	1209:1223	arg1	able					1279:1282	able	1279:1282	able	1279:1282	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	53	dep	gene.IMPORTANCE	1209:1223	arg1	alphaproteobacterium					1229:1248	The alphaproteobacterium	1225:1248	The alphaproteobacterium Agrobacterium tumefaciens	1225:1274	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	54	theme	ampC	818:821	arg1	probing					833:839	ampC Structure probing	818:839	ampC Structure probing	818:839	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	55	theme	efflux	1073:1078	arg1	transporters					1080:1091	efflux transporters	1073:1091	efflux transporters	1073:1091	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	2	56	theme	small	214:218	arg1	sRNA					236:239	sRNA	236:239	sRNA	236:239	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	2	56	theme	small	214:218	arg1	RNA					231:233	a differentially expressed small regulatory RNA	187:233	a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA	187:484	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	5	57	theme	single-stranded	893:907	arg1	loop					909:912	a single-stranded loop	891:912	a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	891:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	58	dep	in	964:965	arg1	vitro					967:971	vitro	967:971	vitro	967:971	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	0	59	theme	Cell	32:35	arg1	Biosynthesis					42:53	Cell Wall Biosynthesis	32:53	Cell Wall Biosynthesis	32:53	A Small Regulatory RNA Controls Cell Wall Biosynthesis and Antibiotic Resistance.					
30425148	7	60	theme	A.	1615:1616	arg1	physiology					1630:1639	A. tumefaciens physiology	1615:1639	A. tumefaciens physiology	1615:1639	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	9	61	from	fitness	1937:1943	arg1	composition					1974:1984	the competitive microbial composition	1948:1984	the competitive microbial composition of the rhizosphere	1948:2003	Among the genes directly targeted by PmaR is ampC coding for a beta-lactamase that confers ampicillin resistance, suggesting that the sRNA is crucial for fitness in the competitive microbial composition of the rhizosphere.					
30425148	0	62	theme	Antibiotic	59:68	arg1	Resistance					70:79	Antibiotic Resistance	59:79	Antibiotic Resistance	59:79	A Small Regulatory RNA Controls Cell Wall Biosynthesis and Antibiotic Resistance.					
30425148	3	63	theme	ampicillin	584:593	arg1	regulator					606:614	ampicillin resistance regulator	584:614	ampicillin resistance regulator	584:614	According to these targets, we named the sRNA PmaR for peptidoglycan biosynthesis, motility, and ampicillin resistance regulator.					
30425148	5	64	theme	sRNA	880:883	arg1	structure					863:871	a spoon-like structure	850:871	a spoon-like structure of the sRNA	850:883	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	7	65	theme	riboregulation	1597:1610	arg1	impact					1587:1592	a considerable impact	1572:1592	a considerable impact of riboregulation on A. tumefaciens physiology	1572:1639	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	5	66	dep	interaction	940:950	arg1	implicated					1006:1015	implicated	1006:1015	have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters	996:1091	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	66	dep	interaction	940:950	arg1	represents					1103:1112	represents	1103:1112	represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	1103:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	6	67	theme	transfer	1396:1403	arg1	ability					1367:1373	its unique ability	1356:1373	its unique ability of interkingdom gene transfer	1356:1403	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	0	68	theme	Regulatory	8:17	arg1	RNA					19:21	A Small Regulatory RNA	0:21	A Small Regulatory RNA	0:21	A Small Regulatory RNA Controls Cell Wall Biosynthesis and Antibiotic Resistance.					
30425148	6	69	theme	interkingdom	1378:1389	arg1	transfer					1396:1403	interkingdom gene transfer	1378:1403	interkingdom gene transfer	1378:1403	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	5	70	theme	in	952:953	arg1	riboregulators					981:994	in vivo and in vitro Several riboregulators	952:994	in vivo and in vitro Several riboregulators	952:994	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	2	71	theme	Agrobacterium	304:316	arg1	tumefaciens					318:328	the plant pathogen Agrobacterium tumefaciens	285:328	the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics	285:404	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	2	72	theme	proteomics	395:404	arg1	combination					338:348	a combination	336:348	a combination of bioinformatic predictions and comparative proteomics	336:404	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	0	73	theme	Small	2:6	arg1	RNA					19:21	A Small Regulatory RNA	0:21	A Small Regulatory RNA	0:21	A Small Regulatory RNA Controls Cell Wall Biosynthesis and Antibiotic Resistance.					
30425148	1	74	theme	Small	82:86	arg1	RNAs					99:102	Small regulatory RNAs	82:102	Small regulatory RNAs	82:102	Small regulatory RNAs play an important role in the adaptation to changing conditions.					
30425148	5	75	theme	crown	1319:1323	arg1	formation					1336:1344	crown gall tumor formation	1319:1344	crown gall tumor formation	1319:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	76	theme	gene	813:816	arg1	regulation					780:789	the positive PmaR-mediated regulation	753:789	the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	753:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	77	theme	target	933:938	arg1	interaction					940:950	target interaction	933:950	target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	933:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	78	theme	tumor	1330:1334	arg1	formation					1336:1344	crown gall tumor formation	1319:1344	crown gall tumor formation	1319:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	79	theme	positive	757:764	arg1	regulation					780:789	the positive PmaR-mediated regulation	753:789	the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation	753:1344	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	7	80	from	impact	1587:1592	arg1	physiology					1630:1639	A. tumefaciens physiology	1615:1639	A. tumefaciens physiology	1615:1639	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	2	81	theme	bioinformatic	353:365	arg1	predictions					367:377	bioinformatic predictions	353:377	bioinformatic predictions	353:377	Here, we describe a differentially expressed small regulatory RNA (sRNA) that affects various cellular processes in the plant pathogen Agrobacterium tumefaciens Using a combination of bioinformatic predictions and comparative proteomics, we identified nine targets, most of which are positively regulated by the sRNA.					
30425148	7	82	theme	sRNAs	1540:1544	arg1	hundreds					1519:1526	hundreds	1519:1526	hundreds of putative sRNAs	1519:1544	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	9	83	theme	ampicillin	1874:1883	arg1	resistance					1885:1894	ampicillin resistance	1874:1894	ampicillin resistance	1874:1894	Among the genes directly targeted by PmaR is ampC coding for a beta-lactamase that confers ampicillin resistance, suggesting that the sRNA is crucial for fitness in the competitive microbial composition of the rhizosphere.					
30425148	7	84	attach	presence	1507:1514	arg2	hundreds					1519:1526	hundreds	1519:1526	hundreds of putative sRNAs	1519:1544	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	7	84	attach	presence	1507:1514	arg1	organism					1554:1561	this organism	1549:1561	this organism	1549:1561	The presence of hundreds of putative sRNAs in this organism suggests a considerable impact of riboregulation on A. tumefaciens physiology.					
30425148	8	85	theme	sRNA	1696:1699	arg1	PmaR					1701:1704	the sRNA PmaR	1692:1704	the sRNA PmaR that controls various processes crucial for growth, motility, and virulence	1692:1780	Here, we characterized the biological function of the sRNA PmaR that controls various processes crucial for growth, motility, and virulence.					
30425148	6	86	theme	genetic	1466:1472	arg1	manipulation					1474:1485	genetic manipulation	1466:1485	genetic manipulation of plant cells	1466:1500	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	6	87	theme	cells	1496:1500	arg1	manipulation					1474:1485	genetic manipulation	1466:1485	genetic manipulation of plant cells	1466:1500	Based on its unique ability of interkingdom gene transfer, Agrobacterium serves as a crucial biotechnological tool for genetic manipulation of plant cells.					
30425148	5	88	theme	Agrobacterium	1250:1262	arg1	able					1279:1282	able	1279:1282	able	1279:1282	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
30425148	5	88	theme	Agrobacterium	1250:1262	arg1	alphaproteobacterium					1229:1248	The alphaproteobacterium	1225:1248	The alphaproteobacterium Agrobacterium tumefaciens	1225:1274	are long known to be naturally resistant to high ampicillin concentrations, and we can now explain this phenotype by the positive PmaR-mediated regulation of the beta-lactamase gene ampC Structure probing revealed a spoon-like structure of the sRNA, with a single-stranded loop that is engaged in target interaction in vivo and in vitro Several riboregulators have been implicated in antibiotic resistance mechanisms, such as uptake and efflux transporters, but PmaR represents the first example of an sRNA that directly controls the expression of an antibiotic resistance gene.IMPORTANCE The alphaproteobacterium Agrobacterium tumefaciens is able to infect various eudicots causing crown gall tumor formation.					
29848118	5	0	from	sprouting	905:913	arg1	industrial-scale					921:936	an industrial-scale	918:936	an industrial-scale	918:936	In conclusion, sprouting on an industrial-scale induced mild structural modifications in chickpeas and peas, sufficient to reduce antinutritional factors, without strongly influencing their nutritional quality.					
29848118	4	1	dep	promoted	785:792	arg1	%					854:854	-10%	851:854	-10%	851:854	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	4	1	dep	promoted	785:792	arg1	%					863:863	-37%	860:863	-37%	860:863	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	1	2	theme	green	163:167	arg1	peas					169:172	green peas	163:172	green peas	163:172	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	1	3	theme	antioxidant	243:253	arg1	capacity					255:262	antioxidant capacity	243:262	antioxidant capacity	243:262	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	0	4	from	Effect	0:5	arg1	quality					35:41	nutritional quality	23:41	nutritional quality of pulses	23:51	Effect of sprouting on nutritional quality of pulses.					
29848118	3	5	from	increase	544:551	arg1	protein					556:562	protein	556:562	protein	556:562	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	3	5	from	increase	544:551	arg1	p < .05					637:643	p < .05	637:643	p < .05	637:643	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	3	5	from	increase	544:551	arg1	sugars					591:596	free sugars	586:596	free sugars (in peas; +10% and +80%, respectively, p < .05)	586:644	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	4	6	from	seeds	844:848	arg1	raw					829:831	raw	829:831	raw	829:831	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	4	7	theme	cooked	758:763	arg1	ones					765:768	unsprouted cooked ones	747:768	unsprouted cooked ones	747:768	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	3	8	dep	sprouted	489:496	arg1	raw					485:487	raw	485:487	raw	485:487	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	1	9	theme	peas	169:172	arg1	quality					94:100	the nutritional quality	78:100	the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas	78:172	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	4	10	theme	unsprouted	747:756	arg1	ones					765:768	unsprouted cooked ones	747:768	unsprouted cooked ones	747:768	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	4	11	dep	%	723:723	arg1	p < .05					771:777	p < .05	771:777	p < .05	771:777	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	1	12	theme	mineral	287:293	arg1	content					295:301	mineral content	287:301	mineral content	287:301	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	1	13	theme	starch	265:270	arg1	digestibility					272:284	starch digestibility	265:284	starch digestibility	265:284	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	4	14	dep	favoured	672:679	arg1	%					723:723	-35%	720:723	-35%	720:723	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	6	15	theme	dietary	1184:1190	arg1	patterns					1192:1199	different dietary patterns	1174:1199	different dietary patterns	1174:1199	These products could represent nutritionally interesting ingredients for different dietary patterns as well as for enriched cereal-based foods.					
29848118	2	16	theme	seed	372:375	arg1	structure					377:385	raw seed structure	368:385	raw seed structure	368:385	Sprouting did not deeply affect raw seed structure, although after cooking starch granules appeared more porous and swelled.					
29848118	4	17	dep	%	854:854	arg1	p < .05					880:886	p < .05	880:886	p < .05	880:886	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	1	18	theme	nutritional	82:92	arg1	quality					94:100	the nutritional quality	78:100	the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas	78:172	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	3	19	theme	Chemical	461:468	arg1	composition					470:480	Chemical composition	461:480	Chemical composition of raw sprouted seeds	461:502	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	0	20	theme	sprouting	10:18	arg1	Effect					0:5	Effect	0:5	Effect of sprouting on nutritional quality of pulses.	0:52	Effect of sprouting on nutritional quality of pulses.					
29848118	4	21	theme	phytic	681:686	arg1	leaching					693:700	phytic acid leaching	681:700	phytic acid leaching	681:700	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	2	22	theme	starch	411:416	arg1	granules					418:425	starch granules	411:425	starch granules	411:425	Sprouting did not deeply affect raw seed structure, although after cooking starch granules appeared more porous and swelled.					
29848118	5	23	theme	nutritional	1080:1090	arg1	quality					1092:1098	their nutritional quality	1074:1098	their nutritional quality	1074:1098	In conclusion, sprouting on an industrial-scale induced mild structural modifications in chickpeas and peas, sufficient to reduce antinutritional factors, without strongly influencing their nutritional quality.					
29848118	3	24	theme	seeds	498:502	arg1	composition					470:480	Chemical composition	461:480	Chemical composition of raw sprouted seeds	461:502	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	4	25	theme	cooked	837:842	arg1	seeds					844:848	cooked seeds	837:848	cooked seeds	837:848	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	5	26	theme	mild	946:949	arg1	modifications					962:974	mild structural modifications	946:974	mild structural modifications	946:974	In conclusion, sprouting on an industrial-scale induced mild structural modifications in chickpeas and peas, sufficient to reduce antinutritional factors, without strongly influencing their nutritional quality.					
29848118	6	27	theme	interesting	1146:1156	arg1	ingredients					1158:1168	nutritionally interesting ingredients	1132:1168	nutritionally interesting ingredients for different dietary patterns as well as for enriched cereal-based foods	1132:1242	These products could represent nutritionally interesting ingredients for different dietary patterns as well as for enriched cereal-based foods.					
29848118	0	28	theme	nutritional	23:33	arg1	quality					35:41	nutritional quality	23:41	nutritional quality of pulses	23:51	Effect of sprouting on nutritional quality of pulses.					
29848118	5	29	theme	antinutritional	1020:1034	arg1	factors					1036:1042	antinutritional factors	1020:1042	antinutritional factors	1020:1042	In conclusion, sprouting on an industrial-scale induced mild structural modifications in chickpeas and peas, sufficient to reduce antinutritional factors, without strongly influencing their nutritional quality.					
29848118	5	30	theme	structural	951:960	arg1	modifications					962:974	mild structural modifications	946:974	mild structural modifications	946:974	In conclusion, sprouting on an industrial-scale induced mild structural modifications in chickpeas and peas, sufficient to reduce antinutritional factors, without strongly influencing their nutritional quality.					
29848118	0	31	theme	pulses	46:51	arg1	quality					35:41	nutritional quality	23:41	nutritional quality of pulses	23:51	Effect of sprouting on nutritional quality of pulses.					
29848118	4	32	theme	antioxidant	794:804	arg1	reductions					815:824	antioxidant capacity reductions	794:824	antioxidant capacity reductions in raw	794:831	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	3	33	theme	peas	602:605	arg1	%					611:611	peas; +10%	602:611	peas; +10%	602:611	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	3	34	theme	sprouted	489:496	arg1	seeds					498:502	raw sprouted seeds	485:502	raw sprouted seeds	485:502	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	3	35	from	p < .05	637:643	arg1	%					611:611	peas; +10%	602:611	peas; +10%	602:611	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	3	35	from	p < .05	637:643	arg1	%					620:620	+80%	617:620	+80%	617:620	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	6	36	theme	cereal-based	1225:1236	arg1	foods					1238:1242	enriched cereal-based foods	1216:1242	enriched cereal-based foods	1216:1242	These products could represent nutritionally interesting ingredients for different dietary patterns as well as for enriched cereal-based foods.					
29848118	4	37	from	reductions	815:824	arg1	raw					829:831	raw	829:831	raw	829:831	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	6	38	theme	different	1174:1182	arg1	patterns					1192:1199	different dietary patterns	1174:1199	different dietary patterns	1174:1199	These products could represent nutritionally interesting ingredients for different dietary patterns as well as for enriched cereal-based foods.					
29848118	1	39	theme	industrial-scale	105:120	arg1	chickpeas					149:157	unsprouted chickpeas	138:157	unsprouted chickpeas	138:157	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	6	40	theme	enriched	1216:1223	arg1	foods					1238:1242	enriched cereal-based foods	1216:1242	enriched cereal-based foods	1216:1242	These products could represent nutritionally interesting ingredients for different dietary patterns as well as for enriched cereal-based foods.					
29848118	1	41	theme	unsprouted	138:147	arg1	chickpeas					149:157	unsprouted chickpeas	138:157	unsprouted chickpeas	138:157	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	1	42	theme	sprouted	122:129	arg1	chickpeas					149:157	unsprouted chickpeas	138:157	unsprouted chickpeas	138:157	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	4	43	theme	acid	688:691	arg1	leaching					693:700	phytic acid leaching	681:700	phytic acid leaching	681:700	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	4	44	theme	capacity	806:813	arg1	reductions					815:824	antioxidant capacity reductions	794:824	antioxidant capacity reductions in raw	794:831	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	3	45	dep	protein	556:562	arg1	both					565:568	both	565:568	both	565:568	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	4	46	theme	industrial	651:660	arg1	sprouting					662:670	The industrial sprouting	647:670	The industrial sprouting	647:670	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	4	47	from	%	723:723	arg1	seeds					728:732	seeds	728:732	seeds	728:732	The industrial sprouting favoured phytic acid leaching in cooking water (-35% in seeds, compared to unsprouted cooked ones, p < .05), and promoted antioxidant capacity reductions in raw and cooked seeds (-10% and -37%, respectively, p < .05).					
29848118	2	48	theme	raw	368:370	arg1	structure					377:385	raw seed structure	368:385	raw seed structure	368:385	Sprouting did not deeply affect raw seed structure, although after cooking starch granules appeared more porous and swelled.					
29848118	1	49	theme	chemical	221:228	arg1	composition					230:240	chemical composition	221:240	chemical composition	221:240	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
29848118	3	50	theme	free	586:589	arg1	p < .05					637:643	p < .05	637:643	p < .05	637:643	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	3	50	theme	free	586:589	arg1	sugars					591:596	free sugars	586:596	free sugars (in peas; +10% and +80%, respectively, p < .05)	586:644	Chemical composition of raw sprouted seeds was not strongly affected, excepting an increase in protein (both pulses), and in free sugars (in peas; +10% and +80%, respectively, p < .05).					
29848118	1	51	theme	chickpeas	149:157	arg1	quality					94:100	the nutritional quality	78:100	the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas	78:172	In order to investigate the nutritional quality of industrial-scale sprouted versus unsprouted chickpeas and green peas, before and after cooking, the ultrastructure, chemical composition, antioxidant capacity, starch digestibility, mineral content and accessibility were analysed.					
31576748	0	0	theme	Cholesterol-Lowering	67:86	arg1	Effects					88:94	the Cholesterol-Lowering Effects	63:94	the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice	63:144	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	1	1	from	effects	178:184	arg1	process					282:288	an innovative, chemically controllable degradation process	231:288	an innovative, chemically controllable degradation process	231:288	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	1	1	from	effects	178:184	arg1	metabolism					305:314	cholesterol metabolism	293:314	cholesterol metabolism	293:314	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	1	1	from	effects	178:184	arg1	composition					338:348	the gut microbial composition	320:348	the gut microbial composition	320:348	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	6	2	theme	liver	1001:1005	arg1	7-α-hydroxylase					1019:1033	liver cholesterol 7-α-hydroxylase	1001:1033	liver cholesterol 7-α-hydroxylase	1001:1033	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	6	3	theme	significant	837:847	arg1	correlations					849:860	significant correlations	837:860	significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase	837:1085	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	7	4	from	mechanisms	1206:1215	arg1	metabolism					1246:1255	cholesterol metabolism	1234:1255	cholesterol metabolism	1234:1255	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	4	5	theme	low-density	613:623	arg1	LDL-C					650:654	LDL-C	650:654	LDL-C	650:654	POS reduced serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) in a dose-dependent manner.					
31576748	4	5	theme	low-density	613:623	arg1	lipoprotein-cholesterol					625:647	low-density lipoprotein-cholesterol	613:647	low-density lipoprotein-cholesterol (LDL-C)	613:655	POS reduced serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) in a dose-dependent manner.					
31576748	7	6	theme	hypocholesterolemic	1144:1162	arg1	effects					1164:1170	hypocholesterolemic effects	1144:1170	hypocholesterolemic effects	1144:1170	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	6	7	theme	fatty	932:936	arg1	acids					938:942	short-chain fatty acids	920:942	short-chain fatty acids	920:942	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	1	8	theme	degradation	270:280	arg1	process					282:288	an innovative, chemically controllable degradation process	231:288	an innovative, chemically controllable degradation process	231:288	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	0	9	theme	Pectin	106:111	arg1	Oligosaccharides					113:128	Citrus Pectin Oligosaccharides	99:128	Citrus Pectin Oligosaccharides	99:128	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	7	10	theme	citrus	1220:1225	arg1	POS					1227:1229	citrus POS	1220:1229	citrus POS on cholesterol metabolism	1220:1255	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	7	11	theme	bacterial	1283:1291	arg1	groups					1293:1298	specific bacterial groups	1274:1298	specific bacterial groups together with their metabolites	1274:1330	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	3	12	theme	HF	501:502	arg1	diet					504:507	a HF diet	499:507	a HF diet	499:507	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	6	13	theme	bile	985:988	arg1	acids					990:994	fecal bile acids	979:994	fecal bile acids	979:994	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	1	14	theme	regulatory	167:176	arg1	effects					178:184	the regulatory effects	163:184	the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition	163:348	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	0	15	theme	Citrus	99:104	arg1	Oligosaccharides					113:128	Citrus Pectin Oligosaccharides	99:128	Citrus Pectin Oligosaccharides	99:128	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	5	16	theme	specific	712:719	arg1	groups					731:736	specific bacterial groups	712:736	specific bacterial groups in the feces	712:749	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	4	17	theme	total	586:590	arg1	cholesterol					592:602	serum total cholesterol	580:602	serum total cholesterol (TC)	580:607	POS reduced serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) in a dose-dependent manner.					
31576748	4	17	theme	total	586:590	arg1	TC					605:606	TC	605:606	TC	605:606	POS reduced serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) in a dose-dependent manner.					
31576748	7	18	theme	prepared	1121:1128	arg1	POS					1130:1132	the prepared POS	1117:1132	the prepared POS	1117:1132	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	5	19	from	feces	745:749	arg1	higher					800:805	higher	800:805	higher	800:805	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	5	19	from	feces	745:749	arg1	concentrations					759:772	the concentrations	755:772	the concentrations of their metabolites	755:793	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	5	19	from	feces	745:749	arg1	abundances					698:707	The relative abundances	685:707	The relative abundances of specific bacterial groups in the feces	685:749	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	1	20	from	process	282:288	arg1	oligosaccharides					203:218	citrus pectin oligosaccharides	189:218	citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process	189:288	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	1	20	from	process	282:288	arg1	effects					178:184	the regulatory effects	163:184	the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition	163:348	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	1	20	from	process	282:288	arg1	POS					221:223	POS	221:223	POS	221:223	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	7	21	theme	POS	1227:1229	arg1	mechanisms					1206:1215	the potential regulatory mechanisms	1181:1215	the potential regulatory mechanisms of citrus POS on cholesterol metabolism	1181:1255	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	4	22	theme	dose-dependent	662:675	arg1	manner					677:682	a dose-dependent manner	660:682	a dose-dependent manner	660:682	POS reduced serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) in a dose-dependent manner.					
31576748	4	23	theme	serum	580:584	arg1	cholesterol					592:602	serum total cholesterol	580:602	serum total cholesterol (TC)	580:607	POS reduced serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) in a dose-dependent manner.					
31576748	4	23	theme	serum	580:584	arg1	TC					605:606	TC	605:606	TC	605:606	POS reduced serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) in a dose-dependent manner.					
31576748	6	24	theme	short-chain	920:930	arg1	acids					938:942	short-chain fatty acids	920:942	short-chain fatty acids	920:942	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	7	25	from	metabolism	1246:1255	arg1	mechanisms					1206:1215	the potential regulatory mechanisms	1181:1215	the potential regulatory mechanisms of citrus POS on cholesterol metabolism	1181:1255	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	1	26	theme	citrus	189:194	arg1	oligosaccharides					203:218	citrus pectin oligosaccharides	189:218	citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process	189:288	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	1	26	theme	citrus	189:194	arg1	POS					221:223	POS	221:223	POS	221:223	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	0	27	theme	Gut	12:14	arg1	Microbiota					16:25	Gut Microbiota	12:25	Gut Microbiota	12:25	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	3	28	theme	g/kg	534:537	arg1	POS					551:553	0.15, 0.45, and 0.9 g/kg body weight POS	514:553	0.15, 0.45, and 0.9 g/kg body weight POS	514:553	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	5	29	theme	groups	731:736	arg1	higher					800:805	higher	800:805	higher	800:805	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	5	29	theme	groups	731:736	arg1	concentrations					759:772	the concentrations	755:772	the concentrations of their metabolites	755:793	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	5	29	theme	groups	731:736	arg1	abundances					698:707	The relative abundances	685:707	The relative abundances of specific bacterial groups in the feces	685:749	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	1	30	theme	pectin	196:201	arg1	oligosaccharides					203:218	citrus pectin oligosaccharides	189:218	citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process	189:288	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	1	30	theme	pectin	196:201	arg1	POS					221:223	POS	221:223	POS	221:223	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	1	31	theme	cholesterol	293:303	arg1	metabolism					305:314	cholesterol metabolism	293:314	cholesterol metabolism	293:314	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	0	32	theme	Oligosaccharides	113:128	arg1	Effects					88:94	the Cholesterol-Lowering Effects	63:94	the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice	63:144	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	1	33	theme	oligosaccharides	203:218	arg1	effects					178:184	the regulatory effects	163:184	the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition	163:348	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	3	34	theme	C57BL/6	428:434	arg1	mice					436:439	Four-week-old male C57BL/6 mice	409:439	Four-week-old male C57BL/6 mice	409:439	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	1	35	dep	innovative	234:243	arg1	controllable					257:268	controllable	257:268	controllable	257:268	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	3	36	theme	body	539:542	arg1	POS					551:553	0.15, 0.45, and 0.9 g/kg body weight POS	514:553	0.15, 0.45, and 0.9 g/kg body weight POS	514:553	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	2	37	theme	flora	389:393	arg1	role					366:369	The modulatory role	351:369	The modulatory role of the intestinal flora	351:393	The modulatory role of the intestinal flora was explored.					
31576748	3	38	theme	standard	459:466	arg1	diet					468:471	a standard diet	457:471	a standard diet; a high-fat (HF) diet;	457:494	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	0	39	theme	Microbiota	16:25	arg1	Role					0:3	Role	0:3	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.	0:145	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	0	40	theme	C57BL/6	133:139	arg1	Mice					141:144	C57BL/6 Mice	133:144	C57BL/6 Mice	133:144	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	3	41	theme	weight	544:549	arg1	POS					551:553	0.15, 0.45, and 0.9 g/kg body weight POS	514:553	0.15, 0.45, and 0.9 g/kg body weight POS	514:553	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	5	42	theme	metabolites	783:793	arg1	higher					800:805	higher	800:805	higher	800:805	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	5	42	theme	metabolites	783:793	arg1	concentrations					759:772	the concentrations	755:772	the concentrations of their metabolites	755:793	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	5	42	theme	metabolites	783:793	arg1	abundances					698:707	The relative abundances	685:707	The relative abundances of specific bacterial groups in the feces	685:749	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	0	43	theme	Metabolites	37:47	arg1	Role					0:3	Role	0:3	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.	0:145	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	5	44	theme	POS	814:816	arg1	groups					818:823	the POS groups	810:823	the POS groups	810:823	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	5	45	theme	relative	689:696	arg1	higher					800:805	higher	800:805	higher	800:805	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	5	45	theme	relative	689:696	arg1	abundances					698:707	The relative abundances	685:707	The relative abundances of specific bacterial groups in the feces	685:749	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	2	46	theme	modulatory	355:364	arg1	role					366:369	The modulatory role	351:369	The modulatory role of the intestinal flora	351:393	The modulatory role of the intestinal flora was explored.					
31576748	3	47	theme	male	423:426	arg1	mice					436:439	Four-week-old male C57BL/6 mice	409:439	Four-week-old male C57BL/6 mice	409:439	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	1	48	theme	gut	324:326	arg1	composition					338:348	the gut microbial composition	320:348	the gut microbial composition	320:348	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	6	49	theme	fecal	979:983	arg1	acids					990:994	fecal bile acids	979:994	fecal bile acids	979:994	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	5	50	from	abundances	698:707	arg1	feces					745:749	the feces	741:749	the feces	741:749	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	0	51	dep	Microbiota	16:25	arg1	the					8:10	the	8:10	the	8:10	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	5	52	from	concentrations	759:772	arg1	feces					745:749	the feces	741:749	the feces	741:749	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	6	53	theme	A	1075:1075	arg1	reductase					1077:1085	3-hydroxy-3-methylglutaryl-coenzyme A reductase	1039:1085	3-hydroxy-3-methylglutaryl-coenzyme A reductase	1039:1085	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	1	54	theme	microbial	328:336	arg1	composition					338:348	the gut microbial composition	320:348	the gut microbial composition	320:348	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
31576748	3	55	theme	high-fat	476:483	arg1	diet					490:493	a high-fat (HF) diet	474:493	a standard diet; a high-fat (HF) diet;	457:494	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	6	56	theme	serum	962:966	arg1	TC					968:969	serum TC	962:969	serum TC	962:969	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	3	57	dep	diet	468:471	arg1	diet					490:493	a high-fat (HF) diet	474:493	a standard diet; a high-fat (HF) diet;	457:494	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	7	58	theme	cholesterol	1234:1244	arg1	metabolism					1246:1255	cholesterol metabolism	1234:1255	cholesterol metabolism	1234:1255	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	6	59	theme	3-hydroxy-3-methylglutaryl-coenzyme	1039:1073	arg1	reductase					1077:1085	3-hydroxy-3-methylglutaryl-coenzyme A reductase	1039:1085	3-hydroxy-3-methylglutaryl-coenzyme A reductase	1039:1085	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	7	60	theme	potential	1185:1193	arg1	mechanisms					1206:1215	the potential regulatory mechanisms	1181:1215	the potential regulatory mechanisms of citrus POS on cholesterol metabolism	1181:1255	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	0	61	from	Effects	88:94	arg1	Mice					141:144	C57BL/6 Mice	133:144	C57BL/6 Mice	133:144	Role of the Gut Microbiota and Their Metabolites in Modulating the Cholesterol-Lowering Effects of Citrus Pectin Oligosaccharides in C57BL/6 Mice.					
31576748	7	62	theme	specific	1274:1281	arg1	groups					1293:1298	specific bacterial groups	1274:1298	specific bacterial groups together with their metabolites	1274:1330	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	2	63	theme	intestinal	378:387	arg1	flora					389:393	the intestinal flora	374:393	the intestinal flora	374:393	The modulatory role of the intestinal flora was explored.					
31576748	7	64	theme	regulatory	1195:1204	arg1	mechanisms					1206:1215	the potential regulatory mechanisms	1181:1215	the potential regulatory mechanisms of citrus POS on cholesterol metabolism	1181:1255	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	7	65	from	POS	1227:1229	arg1	metabolism					1246:1255	cholesterol metabolism	1234:1255	cholesterol metabolism	1234:1255	These findings indicate that the prepared POS exhibited hypocholesterolemic effects and that the potential regulatory mechanisms of citrus POS on cholesterol metabolism are modulated by specific bacterial groups together with their metabolites.					
31576748	3	66	theme	Four-week-old	409:421	arg1	mice					436:439	Four-week-old male C57BL/6 mice	409:439	Four-week-old male C57BL/6 mice	409:439	Four-week-old male C57BL/6 mice were fed either a standard diet; a high-fat (HF) diet; or a HF diet with 0.15, 0.45, and 0.9 g/kg body weight POS for 30 days.					
31576748	5	67	from	groups	731:736	arg1	feces					745:749	the feces	741:749	the feces	741:749	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	5	68	theme	bacterial	721:729	arg1	groups					731:736	specific bacterial groups	712:736	specific bacterial groups in the feces	712:749	The relative abundances of specific bacterial groups in the feces and the concentrations of their metabolites were higher in the POS groups.					
31576748	6	69	theme	cholesterol	1007:1017	arg1	7-α-hydroxylase					1019:1033	liver cholesterol 7-α-hydroxylase	1001:1033	liver cholesterol 7-α-hydroxylase	1001:1033	There were significant correlations among Bifidobacterium, Lactobacillus, and Bacteroides and short-chain fatty acids, as well as among serum TC, LDL-C, fecal bile acids, and liver cholesterol 7-α-hydroxylase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase.					
31576748	1	70	theme	innovative	234:243	arg1	process					282:288	an innovative, chemically controllable degradation process	231:288	an innovative, chemically controllable degradation process	231:288	We investigated the regulatory effects of citrus pectin oligosaccharides (POS) from an innovative, chemically controllable degradation process on cholesterol metabolism and the gut microbial composition.					
30560095	3	0	theme	rRv1288	442:448	arg1	proteins					459:466	The purified rRv1288 and rEst proteins	429:466	The purified rRv1288 and rEst proteins	429:466	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
30560095	13	1	theme	cell	1692:1695	arg1	line					1697:1700	Raw264.7 cell line	1683:1700	Raw264.7 cell line	1683:1700	Rv1288 also enhanced the intracellular survival of M. smegmatis in Raw264.7 cell line.					
30560095	10	2	theme	enhanced	1294:1301	arg1	pellicle					1303:1310	enhanced pellicle	1294:1310	enhanced pellicle	1294:1310	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	7	3	located	present	933:939	arg2	Rv1288					910:915	Rv1288	910:915	Rv1288	910:915	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	7	3	located	present	933:939	arg1	fraction					954:961	cell wall fraction	944:961	cell wall fraction of M.tb H37Ra	944:975	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	8	4	theme	cell	1110:1113	arg1	wall					1115:1118	the cell wall	1106:1118	the cell wall	1106:1118	Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall.					
30560095	6	5	theme	Site	813:816	arg1	mutagenesis					827:837	Site directed mutagenesis	813:837	Site directed mutagenesis	813:837	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	0	6	theme	Wall	132:135	arg1	Lipid					137:141	Cell Wall Lipid	127:141	Cell Wall Lipid	127:141	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	13	7	theme	M.	1667:1668	arg1	smegmatis					1670:1678	M. smegmatis	1667:1678	M. smegmatis	1667:1678	Rv1288 also enhanced the intracellular survival of M. smegmatis in Raw264.7 cell line.					
30560095	9	8	theme	rv1288	1135:1140	arg1	Expression					1121:1130	Expression	1121:1130	Expression of rv1288	1121:1140	Expression of rv1288 was up regulated in M.tb under nutrient starved condition.					
30560095	3	9	theme	length	515:520	arg1	esters					522:527	medium chain length esters	502:527	medium chain length esters as optimum substrates	502:549	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
30560095	1	10	theme	hypothetical	164:175	arg1	M.tb					205:208	M.tb	205:208	M.tb	205:208	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	1	10	theme	hypothetical	164:175	arg1	protein					177:183	a conserved hypothetical protein	152:183	a conserved hypothetical protein of M. tuberculosis (M.tb)	152:209	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	1	10	theme	hypothetical	164:175	arg1	Rv1288					144:149	Rv1288	144:149	Rv1288	144:149	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	7	11	from	present	933:939	arg1	fraction					954:961	cell wall fraction	944:961	cell wall fraction of M.tb H37Ra	944:975	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	0	12	theme	tuberculosis	103:114	arg1	Carboxyl-Esterase					68:84	a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase	8:84	a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis	8:114	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	0	12	theme	tuberculosis	103:114	arg1	Rv1288					0:5	Rv1288	0:5	Rv1288	0:5	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	3	13	theme	medium	502:507	arg1	esters					522:527	medium chain length esters	502:527	medium chain length esters as optimum substrates	502:549	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
30560095	5	14	theme	specificity	771:781	arg1	pH					800:801	pH	800:801	pH	800:801	Both rRv1288 and rEst followed the similar patterns of substrate specificity, temperature and pH activity.					
30560095	5	14	theme	specificity	771:781	arg1	temperature					784:794	temperature	784:794	temperature	784:794	Both rRv1288 and rEst followed the similar patterns of substrate specificity, temperature and pH activity.					
30560095	5	14	theme	specificity	771:781	arg1	patterns					749:756	the similar patterns	737:756	the similar patterns of substrate specificity	737:781	Both rRv1288 and rEst followed the similar patterns of substrate specificity, temperature and pH activity.					
30560095	6	15	theme	site	895:898	arg1	residues					900:907	catalytic site residues	885:907	catalytic site residues	885:907	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	6	15	theme	site	895:898	arg1	Asp-391					862:868	Asp-391	862:868	Asp-391	862:868	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	6	15	theme	site	895:898	arg1	His-425					874:880	His-425	874:880	His-425	874:880	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	6	15	theme	site	895:898	arg1	Ser-294					853:859	Ser-294	853:859	Ser-294	853:859	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	7	16	theme	cell	944:947	arg1	fraction					954:961	cell wall fraction	944:961	cell wall fraction of M.tb H37Ra	944:975	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	1	17	theme	tuberculosis	191:202	arg1	M.tb					205:208	M.tb	205:208	M.tb	205:208	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	1	17	theme	tuberculosis	191:202	arg1	protein					177:183	a conserved hypothetical protein	152:183	a conserved hypothetical protein of M. tuberculosis (M.tb)	152:209	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	1	17	theme	tuberculosis	191:202	arg1	Rv1288					144:149	Rv1288	144:149	Rv1288	144:149	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	7	18	theme	H37Ra	971:975	arg1	fraction					954:961	cell wall fraction	944:961	cell wall fraction of M.tb H37Ra	944:975	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	14	19	theme	wall	1753:1756	arg1	Rv1288					1738:1743	Rv1288	1738:1743	Rv1288	1738:1743	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	14	19	theme	wall	1753:1756	arg1	hydrolase					1777:1785	a cell wall localized carboxyl hydrolase	1746:1785	a cell wall localized carboxyl hydrolase with mycolyl-transferase activity	1746:1819	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	9	20	theme	starved	1182:1188	arg1	condition					1190:1198	nutrient starved condition	1173:1198	nutrient starved condition	1173:1198	Expression of rv1288 was up regulated in M.tb under nutrient starved condition.					
30560095	0	21	theme	Anchored	32:39	arg1	Carboxyl-Esterase					68:84	a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase	8:84	a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis	8:114	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	0	21	theme	Anchored	32:39	arg1	Rv1288					0:5	Rv1288	0:5	Rv1288	0:5	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	4	22	theme	domain	627:632	arg1	presence					611:618	presence	611:618	presence of Lyt domain	611:632	However, presence of Lyt domain resulted in enhanced rate of protein aggregation at higher temperature.					
30560095	12	23	theme	M.	1602:1603	arg1	smegmatis					1605:1613	M. smegmatis	1602:1613	M. smegmatis	1602:1613	Rv1288 also leads to increase in drug resistance of M. smegmatis.					
30560095	11	24	theme	smegmatis	1432:1440	arg1	wall					1421:1424	Cell wall	1416:1424	Cell wall of M. smegmatis expressing rv1288	1416:1458	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	2	25	dep	Rv1288	311:316	arg1	Est					335:337	Est	335:337	Est	335:337	In the present study, Rv1288 and its domains (Est and Lyt) were cloned individually from M.tb into E. coli for expression and purification.					
30560095	2	25	dep	Rv1288	311:316	arg1	Lyt					343:345	Lyt	343:345	Lyt	343:345	In the present study, Rv1288 and its domains (Est and Lyt) were cloned individually from M.tb into E. coli for expression and purification.					
30560095	14	26	theme	bacteria	1878:1885	arg1	survival					1866:1873	the survival	1862:1873	the survival of bacteria	1862:1885	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	4	27	from	temperature	693:703	arg1	rate					655:658	enhanced rate	646:658	enhanced rate of protein aggregation at higher temperature	646:703	However, presence of Lyt domain resulted in enhanced rate of protein aggregation at higher temperature.					
30560095	10	28	theme	lipid	1374:1378	arg1	composition					1380:1390	the changed lipid composition	1362:1390	the changed lipid composition of bacterial cell wall	1362:1413	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	4	29	theme	protein	663:669	arg1	aggregation					671:681	protein aggregation	663:681	protein aggregation	663:681	However, presence of Lyt domain resulted in enhanced rate of protein aggregation at higher temperature.					
30560095	5	30	theme	similar	741:747	arg1	pH					800:801	pH	800:801	pH	800:801	Both rRv1288 and rEst followed the similar patterns of substrate specificity, temperature and pH activity.					
30560095	5	30	theme	similar	741:747	arg1	temperature					784:794	temperature	784:794	temperature	784:794	Both rRv1288 and rEst followed the similar patterns of substrate specificity, temperature and pH activity.					
30560095	5	30	theme	similar	741:747	arg1	patterns					749:756	the similar patterns	737:756	the similar patterns of substrate specificity	737:781	Both rRv1288 and rEst followed the similar patterns of substrate specificity, temperature and pH activity.					
30560095	1	31	theme	two-domain	242:251	arg1	enzyme					262:267	two-domain esterase enzyme	242:267	two-domain esterase enzyme	242:267	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	1	31	theme	two-domain	242:251	arg1	Rv1288					144:149	Rv1288	144:149	Rv1288	144:149	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	8	32	theme	binding	992:998	arg1	activity					1000:1007	Peptidoglycan binding activity	978:1007	Peptidoglycan binding activity of Rv1288 and its domains	978:1033	Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall.					
30560095	14	33	theme	stress	1893:1898	arg1	condition					1900:1908	stress condition	1893:1908	stress condition	1893:1908	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	10	34	theme	surrogate	1230:1238	arg1	smegmatis					1248:1256	surrogate host M. smegmatis	1230:1256	surrogate host M. smegmatis	1230:1256	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	0	35	dep	Anchored	32:39	arg1	Inducible					58:66	Inducible	58:66	Inducible	58:66	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	0	35	dep	Anchored	32:39	arg1	Wall					27:30	Two Domain, Cell Wall	10:30	Wall	27:30	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	10	36	from	change	1265:1270	arg1	pellicle					1303:1310	enhanced pellicle	1294:1310	enhanced pellicle	1294:1310	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	10	36	from	change	1265:1270	arg1	formation					1326:1334	aggregate formation	1316:1334	aggregate formation	1316:1334	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	10	36	from	change	1265:1270	arg1	morphology					1282:1291	colony morphology	1275:1291	colony morphology	1275:1291	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	10	37	theme	cell	1405:1408	arg1	wall					1410:1413	bacterial cell wall	1395:1413	bacterial cell wall	1395:1413	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	3	38	theme	enzymatic	582:590	arg1	activity					592:599	enzymatic activity	582:599	enzymatic activity	582:599	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
30560095	10	39	theme	rv1288	1220:1225	arg1	expression					1206:1215	expression	1206:1215	expression of rv1288 in surrogate host M. smegmatis	1206:1256	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	0	40	theme	Domain	14:19	arg1	Wall					27:30	Two Domain, Cell Wall	10:30	Wall	27:30	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	1	41	theme	in	272:273	arg1	study					282:286	in silico study	272:286	in silico study	272:286	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	14	42	with	hydrolase	1777:1785	arg1	activity					1812:1819	mycolyl-transferase activity	1792:1819	mycolyl-transferase activity	1792:1819	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	11	43	theme	significant	1496:1506	arg1	increase					1508:1515	a significant increase	1494:1515	a significant increase in trehalose dimycolate content	1494:1547	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	8	44	theme	domains	1027:1033	arg1	activity					1000:1007	Peptidoglycan binding activity	978:1007	Peptidoglycan binding activity of Rv1288 and its domains	978:1033	Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall.					
30560095	10	45	theme	M.	1245:1246	arg1	smegmatis					1248:1256	surrogate host M. smegmatis	1230:1256	surrogate host M. smegmatis	1230:1256	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	10	46	theme	colony	1275:1280	arg1	morphology					1282:1291	colony morphology	1275:1291	colony morphology	1275:1291	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	8	47	theme	Lyt	1057:1059	arg1	essential					1071:1079	essential	1071:1079	essential	1071:1079	Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall.					
30560095	8	47	theme	Lyt	1057:1059	arg1	domain					1061:1066	the Lyt domain	1053:1066	the Lyt domain	1053:1066	Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall.					
30560095	14	48	theme	cell	1836:1839	arg1	lipids					1846:1851	the cell wall lipids	1832:1851	the cell wall lipids	1832:1851	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	3	49	theme	purified	433:440	arg1	proteins					459:466	The purified rRv1288 and rEst proteins	429:466	The purified rRv1288 and rEst proteins	429:466	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
30560095	13	50	theme	Raw264.7	1683:1690	arg1	line					1697:1700	Raw264.7 cell line	1683:1700	Raw264.7 cell line	1683:1700	Rv1288 also enhanced the intracellular survival of M. smegmatis in Raw264.7 cell line.					
30560095	3	51	theme	lipolytic	478:486	arg1	activity					488:495	lipolytic activity	478:495	lipolytic activity	478:495	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
30560095	12	52	from	increase	1571:1578	arg1	resistance					1588:1597	drug resistance	1583:1597	drug resistance of M. smegmatis	1583:1613	Rv1288 also leads to increase in drug resistance of M. smegmatis.					
30560095	11	53	from	increase	1508:1515	arg1	content					1541:1547	trehalose dimycolate content	1520:1547	trehalose dimycolate content	1520:1547	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	6	54	theme	directed	818:825	arg1	mutagenesis					827:837	Site directed mutagenesis	813:837	Site directed mutagenesis	813:837	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	11	55	theme	lipids	1481:1486	arg1	amount					1471:1476	higher amount	1464:1476	higher amount of lipids	1464:1486	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	11	55	theme	lipids	1481:1486	arg1	lipids					1481:1486	lipids	1481:1486	lipids	1481:1486	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	3	56	theme	chain	509:513	arg1	esters					522:527	medium chain length esters	502:527	medium chain length esters as optimum substrates	502:549	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
30560095	1	57	theme	conserved	154:162	arg1	M.tb					205:208	M.tb	205:208	M.tb	205:208	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	1	57	theme	conserved	154:162	arg1	protein					177:183	a conserved hypothetical protein	152:183	a conserved hypothetical protein of M. tuberculosis (M.tb)	152:209	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	1	57	theme	conserved	154:162	arg1	Rv1288					144:149	Rv1288	144:149	Rv1288	144:149	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	13	58	theme	smegmatis	1670:1678	arg1	survival					1655:1662	the intracellular survival	1637:1662	the intracellular survival of M. smegmatis in Raw264.7 cell line	1637:1700	Rv1288 also enhanced the intracellular survival of M. smegmatis in Raw264.7 cell line.					
30560095	11	59	theme	higher	1464:1469	arg1	amount					1471:1476	higher amount	1464:1476	higher amount of lipids	1464:1486	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	11	59	theme	higher	1464:1469	arg1	lipids					1481:1486	lipids	1481:1486	lipids	1481:1486	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	10	60	theme	aggregate	1316:1324	arg1	formation					1326:1334	aggregate formation	1316:1334	aggregate formation	1316:1334	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	5	61	theme	substrate	761:769	arg1	specificity					771:781	substrate specificity	761:781	substrate specificity	761:781	Both rRv1288 and rEst followed the similar patterns of substrate specificity, temperature and pH activity.					
30560095	0	62	theme	Cell	127:130	arg1	Lipid					137:141	Cell Wall Lipid	127:141	Cell Wall Lipid	127:141	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	3	63	theme	optimum	532:538	arg1	substrates					540:549	optimum substrates	532:549	optimum substrates	532:549	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
30560095	1	64	theme	M.	188:189	arg1	tuberculosis					191:202	M. tuberculosis	188:202	M. tuberculosis	188:202	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	6	65	theme	catalytic	885:893	arg1	residues					900:907	catalytic site residues	885:907	catalytic site residues	885:907	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	6	65	theme	catalytic	885:893	arg1	Asp-391					862:868	Asp-391	862:868	Asp-391	862:868	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	6	65	theme	catalytic	885:893	arg1	His-425					874:880	His-425	874:880	His-425	874:880	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	6	65	theme	catalytic	885:893	arg1	Ser-294					853:859	Ser-294	853:859	Ser-294	853:859	Site directed mutagenesis confirmed the Ser-294, Asp-391 and His-425 as catalytic site residues.					
30560095	13	66	theme	intracellular	1641:1653	arg1	survival					1655:1662	the intracellular survival	1637:1662	the intracellular survival of M. smegmatis in Raw264.7 cell line	1637:1700	Rv1288 also enhanced the intracellular survival of M. smegmatis in Raw264.7 cell line.					
30560095	7	67	theme	M.tb	966:969	arg1	H37Ra					971:975	M.tb H37Ra	966:975	M.tb H37Ra	966:975	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	7	68	theme	wall	949:952	arg1	fraction					954:961	cell wall fraction	944:961	cell wall fraction of M.tb H37Ra	944:975	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	14	69	theme	localized	1758:1766	arg1	Rv1288					1738:1743	Rv1288	1738:1743	Rv1288	1738:1743	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	14	69	theme	localized	1758:1766	arg1	hydrolase					1777:1785	a cell wall localized carboxyl hydrolase	1746:1785	a cell wall localized carboxyl hydrolase with mycolyl-transferase activity	1746:1819	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	10	70	from	expression	1206:1215	arg1	smegmatis					1248:1256	surrogate host M. smegmatis	1230:1256	surrogate host M. smegmatis	1230:1256	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	9	71	theme	nutrient	1173:1180	arg1	condition					1190:1198	nutrient starved condition	1173:1198	nutrient starved condition	1173:1198	Expression of rv1288 was up regulated in M.tb under nutrient starved condition.					
30560095	3	72	theme	Lyt	558:560	arg1	domain					562:567	Lyt domain	558:567	Lyt domain	558:567	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
30560095	14	73	theme	cell	1748:1751	arg1	Rv1288					1738:1743	Rv1288	1738:1743	Rv1288	1738:1743	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	14	73	theme	cell	1748:1751	arg1	hydrolase					1777:1785	a cell wall localized carboxyl hydrolase	1746:1785	a cell wall localized carboxyl hydrolase with mycolyl-transferase activity	1746:1819	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	12	74	theme	smegmatis	1605:1613	arg1	resistance					1588:1597	drug resistance	1583:1597	drug resistance of M. smegmatis	1583:1613	Rv1288 also leads to increase in drug resistance of M. smegmatis.					
30560095	4	75	theme	enhanced	646:653	arg1	rate					655:658	enhanced rate	646:658	enhanced rate of protein aggregation at higher temperature	646:703	However, presence of Lyt domain resulted in enhanced rate of protein aggregation at higher temperature.					
30560095	14	76	theme	mycolyl-transferase	1792:1810	arg1	activity					1812:1819	mycolyl-transferase activity	1792:1819	mycolyl-transferase activity	1792:1819	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	13	77	from	survival	1655:1662	arg1	line					1697:1700	Raw264.7 cell line	1683:1700	Raw264.7 cell line	1683:1700	Rv1288 also enhanced the intracellular survival of M. smegmatis in Raw264.7 cell line.					
30560095	12	78	theme	drug	1583:1586	arg1	resistance					1588:1597	drug resistance	1583:1597	drug resistance of M. smegmatis	1583:1613	Rv1288 also leads to increase in drug resistance of M. smegmatis.					
30560095	4	79	theme	Lyt	623:625	arg1	domain					627:632	Lyt domain	623:632	Lyt domain	623:632	However, presence of Lyt domain resulted in enhanced rate of protein aggregation at higher temperature.					
30560095	11	80	theme	Cell	1416:1419	arg1	wall					1421:1424	Cell wall	1416:1424	Cell wall of M. smegmatis expressing rv1288	1416:1458	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	11	81	contain	had	1460:1462	arg2	lipids					1481:1486	lipids	1481:1486	lipids	1481:1486	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	11	81	contain	had	1460:1462	arg1	wall					1421:1424	Cell wall	1416:1424	Cell wall of M. smegmatis expressing rv1288	1416:1458	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	11	81	contain	had	1460:1462	arg2	amount					1471:1476	higher amount	1464:1476	higher amount of lipids	1464:1486	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	4	82	theme	higher	686:691	arg1	temperature					693:703	higher temperature	686:703	higher temperature	686:703	However, presence of Lyt domain resulted in enhanced rate of protein aggregation at higher temperature.					
30560095	0	83	theme	Mycobacterium	89:101	arg1	tuberculosis					103:114	Mycobacterium tuberculosis	89:114	Mycobacterium tuberculosis	89:114	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	8	84	theme	Peptidoglycan	978:990	arg1	activity					1000:1007	Peptidoglycan binding activity	978:1007	Peptidoglycan binding activity of Rv1288 and its domains	978:1033	Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall.					
30560095	7	85	attach	present	933:939	arg2	Rv1288					910:915	Rv1288	910:915	Rv1288	910:915	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	7	85	attach	present	933:939	arg1	fraction					954:961	cell wall fraction	944:961	cell wall fraction of M.tb H37Ra	944:975	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	1	86	dep	in	272:273	arg1	silico					275:280	silico	275:280	silico	275:280	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	10	87	theme	changed	1366:1372	arg1	composition					1380:1390	the changed lipid composition	1362:1390	the changed lipid composition of bacterial cell wall	1362:1413	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	10	88	theme	host	1240:1243	arg1	smegmatis					1248:1256	surrogate host M. smegmatis	1230:1256	surrogate host M. smegmatis	1230:1256	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	1	89	theme	esterase	253:260	arg1	enzyme					262:267	two-domain esterase enzyme	242:267	two-domain esterase enzyme	242:267	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	1	89	theme	esterase	253:260	arg1	Rv1288					144:149	Rv1288	144:149	Rv1288	144:149	Rv1288, a conserved hypothetical protein of M. tuberculosis (M.tb), was recently characterized as two-domain esterase enzyme by in silico study.					
30560095	8	90	theme	Rv1288	1012:1017	arg1	activity					1000:1007	Peptidoglycan binding activity	978:1007	Peptidoglycan binding activity of Rv1288 and its domains	978:1033	Peptidoglycan binding activity of Rv1288 and its domains demonstrated that the Lyt domain is essential for anchoring protein to the cell wall.					
30560095	11	91	theme	dimycolate	1530:1539	arg1	content					1541:1547	trehalose dimycolate content	1520:1547	trehalose dimycolate content	1520:1547	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	10	92	theme	wall	1410:1413	arg1	composition					1380:1390	the changed lipid composition	1362:1390	the changed lipid composition of bacterial cell wall	1362:1413	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	4	93	theme	aggregation	671:681	arg1	rate					655:658	enhanced rate	646:658	enhanced rate of protein aggregation at higher temperature	646:703	However, presence of Lyt domain resulted in enhanced rate of protein aggregation at higher temperature.					
30560095	0	94	dep	Wall	27:30	arg1	Cell					22:25	Two Domain, Cell Wall	10:30	Cell	22:25	Rv1288, a Two Domain, Cell Wall Anchored, Nutrient Stress Inducible Carboxyl-Esterase of Mycobacterium tuberculosis, Modulates Cell Wall Lipid.					
30560095	10	95	theme	bacterial	1395:1403	arg1	wall					1410:1413	bacterial cell wall	1395:1413	bacterial cell wall	1395:1413	Over expression of rv1288 in surrogate host M. smegmatis led to change in colony morphology, enhanced pellicle and aggregate formation that might be linked with the changed lipid composition of bacterial cell wall.					
30560095	2	96	theme	present	296:302	arg1	study					304:308	the present study	292:308	the present study	292:308	In the present study, Rv1288 and its domains (Est and Lyt) were cloned individually from M.tb into E. coli for expression and purification.					
30560095	7	97	from	fraction	954:961	arg1	present					933:939	present	933:939	present	933:939	Rv1288 was found to be present in cell wall fraction of M.tb H37Ra.					
30560095	5	98	dep	temperature	784:794	arg1	activity					803:810	activity	803:810	activity	803:810	Both rRv1288 and rEst followed the similar patterns of substrate specificity, temperature and pH activity.					
30560095	11	99	theme	trehalose	1520:1528	arg1	content					1541:1547	trehalose dimycolate content	1520:1547	trehalose dimycolate content	1520:1547	Cell wall of M. smegmatis expressing rv1288 had higher amount of lipids, with a significant increase in trehalose dimycolate content.					
30560095	14	100	theme	carboxyl	1768:1775	arg1	Rv1288					1738:1743	Rv1288	1738:1743	Rv1288	1738:1743	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	14	100	theme	carboxyl	1768:1775	arg1	hydrolase					1777:1785	a cell wall localized carboxyl hydrolase	1746:1785	a cell wall localized carboxyl hydrolase with mycolyl-transferase activity	1746:1819	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	14	101	theme	wall	1841:1844	arg1	lipids					1846:1851	the cell wall lipids	1832:1851	the cell wall lipids	1832:1851	Overall, this study suggested that Rv1288, a cell wall localized carboxyl hydrolase with mycolyl-transferase activity, modulated the cell wall lipids to favor the survival of bacteria under stress condition.					
30560095	3	102	theme	rEst	454:457	arg1	proteins					459:466	The purified rRv1288 and rEst proteins	429:466	The purified rRv1288 and rEst proteins	429:466	The purified rRv1288 and rEst proteins exhibited lipolytic activity with medium chain length esters as optimum substrates, while Lyt domain did not show enzymatic activity.					
31587902	7	0	theme	iron	1071:1074	arg1	status					1076:1081	iron status	1071:1081	iron status	1071:1081	However, 5-ALA did not affect iron status or plasma biochemical composition.					
31587902	0	1	theme	supplementation	33:47	arg1	Effects					0:6	Effects	0:6	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows	0:114	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	7	2	theme	biochemical	1093:1103	arg1	composition					1105:1115	plasma biochemical composition	1086:1115	plasma biochemical composition	1086:1115	However, 5-ALA did not affect iron status or plasma biochemical composition.					
31587902	1	3	theme	immune	289:294	arg1	response					296:303	immune response	289:303	immune response	289:303	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	4	theme	study	139:143	arg1	objective					121:129	The objective	117:129	The objective of this study	117:143	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	5	theme	dietary	213:219	arg1	supplement					221:230	a dietary supplement	211:230	a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows	211:327	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	0	6	theme	immune	86:91	arg1	response					93:100	immune response	86:100	immune response of dairy cows	86:114	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	0	7	from	Effects	0:6	arg1	status					74:79	iron status	69:79	iron status	69:79	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	0	7	from	Effects	0:6	arg1	response					93:100	immune response	86:100	immune response of dairy cows	86:114	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	0	7	from	Effects	0:6	arg1	production					57:66	milk production	52:66	milk production	52:66	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	6	8	from	phagocytosis	895:906	arg1	cows					983:986	cows	983:986	cows fed 5-ALA	983:996	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	9	9	theme	lactating	1371:1379	arg1	cows					1387:1390	lactating dairy cows	1371:1390	lactating dairy cows	1371:1390	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	6	10	theme	mitogen-induced	912:926	arg1	proliferation					928:940	mitogen-induced proliferation	912:940	mitogen-induced proliferation	912:940	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	8	11	theme	milk	1222:1225	arg1	production					1227:1236	milk production	1222:1236	milk production	1222:1236	Supplementation with 5-ALA improved milk protein and milk casein contents; however, it had no effect on milk production, milk fat, lactose, total solids, or solids-not-fat, compared with the control.					
31587902	9	12	theme	dietary	1335:1341	arg1	supplementation					1343:1357	dietary supplementation	1335:1357	dietary supplementation of 5-ALA to lactating dairy cows	1335:1390	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	9	13	theme	dairy	1381:1385	arg1	cows					1387:1390	lactating dairy cows	1371:1390	lactating dairy cows	1371:1390	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	6	14	from	cells	974:978	arg1	cows					983:986	cows	983:986	cows fed 5-ALA	983:996	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	5	15	theme	granulocytes	837:848	arg1	counts					805:810	increased counts	795:810	increased counts of white blood cells and granulocytes	795:848	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	5	16	theme	d.	734:735	arg1	cows					743:746	2 d. Dairy cows	732:746	2 d. Dairy cows fed the diet supplemented with 5-ALA	732:783	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	1	17	theme	lactating	308:316	arg1	cows					324:327	lactating dairy cows	308:327	lactating dairy cows	308:327	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	6	18	theme	proliferation	928:940	arg1	rate					887:890	The rate	883:890	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA	883:996	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	6	18	theme	proliferation	928:940	arg1	higher					1003:1008	higher	1003:1008	higher	1003:1008	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	6	19	theme	blood	956:960	arg1	cells					974:978	peripheral blood mononuclear cells	945:978	peripheral blood mononuclear cells in cows fed 5-ALA	945:996	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	5	20	theme	adaptation	685:694	arg1	period					696:701	an adaptation period	682:701	an adaptation period of 12 d	682:709	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	8	21	contain	had	1205:1207	arg1	it					1202:1203	it	1202:1203	it	1202:1203	Supplementation with 5-ALA improved milk protein and milk casein contents; however, it had no effect on milk production, milk fat, lactose, total solids, or solids-not-fat, compared with the control.					
31587902	8	21	contain	had	1205:1207	arg2	effect					1212:1217	no effect	1209:1217	no effect	1209:1217	Supplementation with 5-ALA improved milk protein and milk casein contents; however, it had no effect on milk production, milk fat, lactose, total solids, or solids-not-fat, compared with the control.					
31587902	1	22	theme	dairy	318:322	arg1	cows					324:327	lactating dairy cows	308:327	lactating dairy cows	308:327	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	5	23	theme	d	709:709	arg1	period					696:701	an adaptation period	682:701	an adaptation period of 12 d	682:709	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	5	23	theme	d	709:709	arg1	period					722:727	a test period	715:727	a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA	715:783	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	5	24	theme	Dairy	737:741	arg1	cows					743:746	2 d. Dairy cows	732:746	2 d. Dairy cows fed the diet supplemented with 5-ALA	732:783	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	9	25	theme	5-ALA	1362:1366	arg1	supplementation					1343:1357	dietary supplementation	1335:1357	dietary supplementation of 5-ALA to lactating dairy cows	1335:1390	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	6	26	theme	cells	974:978	arg1	proliferation					928:940	mitogen-induced proliferation	912:940	mitogen-induced proliferation	912:940	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	6	26	theme	cells	974:978	arg1	phagocytosis					895:906	phagocytosis	895:906	phagocytosis	895:906	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	6	27	theme	basal	1029:1033	arg1	diet					1035:1038	a basal diet	1027:1038	a basal diet	1027:1038	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	2	28	theme	matter	494:499	arg1	kilogram					478:485	kilogram	478:485	kilogram of dry matter	478:499	In this study 13 lactating Holstein cows were randomly assigned to either a control group or a treatment group supplemented with 10 mg of 5-ALA per kilogram of dry matter.					
31587902	5	29	theme	cows	743:746	arg1	period					696:701	an adaptation period	682:701	an adaptation period of 12 d	682:709	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	5	29	theme	cows	743:746	arg1	period					722:727	a test period	715:727	a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA	715:783	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	3	30	theme	top-dressed	584:594	arg1	top-dressed					584:594	top-dressed	584:594	top-dressed	584:594	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	3	30	theme	top-dressed	584:594	arg1	ration					573:578	the total mixed ration	557:578	the total mixed ration	557:578	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	3	30	theme	top-dressed	584:594	arg1	amount					547:552	a small amount	539:552	a small amount of the total mixed ration and top-dressed	539:594	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	1	31	theme	milk	235:238	arg1	yield					240:244	milk yield	235:244	milk yield	235:244	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	5	32	theme	white	815:819	arg1	cells					827:831	white blood cells	815:831	white blood cells	815:831	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	6	33	theme	peripheral	945:954	arg1	cells					974:978	peripheral blood mononuclear cells	945:978	peripheral blood mononuclear cells in cows fed 5-ALA	945:996	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	0	34	theme	acid	28:31	arg1	supplementation					33:47	5-aminolevulinic acid supplementation	11:47	5-aminolevulinic acid supplementation	11:47	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	1	35	from	supplement	221:230	arg1	composition					250:260	composition	250:260	composition	250:260	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	35	from	supplement	221:230	arg1	yield					240:244	milk yield	235:244	milk yield	235:244	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	35	from	supplement	221:230	arg1	cows					324:327	lactating dairy cows	308:327	lactating dairy cows	308:327	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	6	36	from	proliferation	928:940	arg1	cows					983:986	cows	983:986	cows fed 5-ALA	983:996	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	5	37	theme	blood	821:825	arg1	cells					827:831	white blood cells	815:831	white blood cells	815:831	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	5	38	theme	test	717:720	arg1	period					722:727	a test period	715:727	a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA	715:783	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	0	39	theme	5-aminolevulinic	11:26	arg1	supplementation					33:47	5-aminolevulinic acid supplementation	11:47	5-aminolevulinic acid supplementation	11:47	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	0	40	theme	dairy	105:109	arg1	cows					111:114	dairy cows	105:114	dairy cows	105:114	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	5	41	theme	cells	827:831	arg1	counts					805:810	increased counts	795:810	increased counts of white blood cells and granulocytes	795:848	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	2	42	theme	Holstein	357:364	arg1	cows					366:369	13 lactating Holstein cows	344:369	13 lactating Holstein cows	344:369	In this study 13 lactating Holstein cows were randomly assigned to either a control group or a treatment group supplemented with 10 mg of 5-ALA per kilogram of dry matter.					
31587902	2	43	theme	5-ALA	468:472	arg1	mg					462:463	10 mg	459:463	10 mg of 5-ALA per kilogram of dry matter	459:499	In this study 13 lactating Holstein cows were randomly assigned to either a control group or a treatment group supplemented with 10 mg of 5-ALA per kilogram of dry matter.					
31587902	2	44	theme	lactating	347:355	arg1	cows					366:369	13 lactating Holstein cows	344:369	13 lactating Holstein cows	344:369	In this study 13 lactating Holstein cows were randomly assigned to either a control group or a treatment group supplemented with 10 mg of 5-ALA per kilogram of dry matter.					
31587902	1	45	theme	5-aminolevulinic	178:193	arg1	5-ALA					201:205	5-ALA	201:205	5-ALA	201:205	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	45	theme	5-aminolevulinic	178:193	arg1	acid					195:198	5-aminolevulinic acid	178:198	5-aminolevulinic acid (5-ALA)	178:206	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	46	from	response	296:303	arg1	composition					250:260	composition	250:260	composition	250:260	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	46	from	response	296:303	arg1	yield					240:244	milk yield	235:244	milk yield	235:244	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	46	from	response	296:303	arg1	cows					324:327	lactating dairy cows	308:327	lactating dairy cows	308:327	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	2	47	theme	control	406:412	arg1	group					414:418	a control group	404:418	a control group	404:418	In this study 13 lactating Holstein cows were randomly assigned to either a control group or a treatment group supplemented with 10 mg of 5-ALA per kilogram of dry matter.					
31587902	3	48	theme	small	541:545	arg1	top-dressed					584:594	top-dressed	584:594	top-dressed	584:594	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	3	48	theme	small	541:545	arg1	ration					573:578	the total mixed ration	557:578	the total mixed ration	557:578	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	3	48	theme	small	541:545	arg1	amount					547:552	a small amount	539:552	a small amount of the total mixed ration and top-dressed	539:594	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	8	49	theme	milk	1239:1242	arg1	fat					1244:1246	milk fat	1239:1246	milk fat	1239:1246	Supplementation with 5-ALA improved milk protein and milk casein contents; however, it had no effect on milk production, milk fat, lactose, total solids, or solids-not-fat, compared with the control.					
31587902	8	50	theme	milk	1171:1174	arg1	contents					1183:1190	milk casein contents	1171:1190	milk casein contents	1171:1190	Supplementation with 5-ALA improved milk protein and milk casein contents; however, it had no effect on milk production, milk fat, lactose, total solids, or solids-not-fat, compared with the control.					
31587902	1	51	theme	acid	195:198	arg1	effect					168:173	the effect	164:173	the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows	164:327	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	52	from	status	278:283	arg1	composition					250:260	composition	250:260	composition	250:260	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	52	from	status	278:283	arg1	yield					240:244	milk yield	235:244	milk yield	235:244	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	1	52	from	status	278:283	arg1	cows					324:327	lactating dairy cows	308:327	lactating dairy cows	308:327	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	9	53	contain	have	1396:1399	arg2	effect					1412:1417	a positive effect	1401:1417	a positive effect	1401:1417	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	9	53	contain	have	1396:1399	arg1	supplementation					1343:1357	dietary supplementation	1335:1357	dietary supplementation of 5-ALA to lactating dairy cows	1335:1390	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	7	54	theme	plasma	1086:1091	arg1	composition					1105:1115	plasma biochemical composition	1086:1115	plasma biochemical composition	1086:1115	However, 5-ALA did not affect iron status or plasma biochemical composition.					
31587902	9	55	theme	protein	1427:1433	arg1	synthesis					1435:1443	milk protein synthesis	1422:1443	milk protein synthesis	1422:1443	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	0	56	theme	milk	52:55	arg1	production					57:66	milk production	52:66	milk production	52:66	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	0	57	theme	cows	111:114	arg1	status					74:79	iron status	69:79	iron status	69:79	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	0	57	theme	cows	111:114	arg1	response					93:100	immune response	86:100	immune response of dairy cows	86:114	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	0	57	theme	cows	111:114	arg1	production					57:66	milk production	52:66	milk production	52:66	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	3	58	theme	ration	573:578	arg1	top-dressed					584:594	top-dressed	584:594	top-dressed	584:594	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	3	58	theme	ration	573:578	arg1	ration					573:578	the total mixed ration	557:578	the total mixed ration	557:578	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	3	58	theme	ration	573:578	arg1	amount					547:552	a small amount	539:552	a small amount of the total mixed ration and top-dressed	539:594	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	1	59	theme	iron	273:276	arg1	status					278:283	iron status	273:283	iron status	273:283	The objective of this study was to investigate the effect of 5-aminolevulinic acid (5-ALA) as a dietary supplement on milk yield and composition as well as iron status and immune response in lactating dairy cows.					
31587902	2	60	theme	dry	490:492	arg1	matter					494:499	dry matter	490:499	dry matter	490:499	In this study 13 lactating Holstein cows were randomly assigned to either a control group or a treatment group supplemented with 10 mg of 5-ALA per kilogram of dry matter.					
31587902	8	61	theme	total	1258:1262	arg1	solids					1264:1269	total solids	1258:1269	total solids	1258:1269	Supplementation with 5-ALA improved milk protein and milk casein contents; however, it had no effect on milk production, milk fat, lactose, total solids, or solids-not-fat, compared with the control.					
31587902	9	62	theme	milk	1422:1425	arg1	synthesis					1435:1443	milk protein synthesis	1422:1443	milk protein synthesis	1422:1443	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	5	63	theme	increased	795:803	arg1	counts					805:810	increased counts	795:810	increased counts of white blood cells and granulocytes	795:848	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	9	64	theme	positive	1403:1410	arg1	effect					1412:1417	a positive effect	1401:1417	a positive effect	1401:1417	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	6	65	theme	phagocytosis	895:906	arg1	rate					887:890	The rate	883:890	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA	883:996	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	6	65	theme	phagocytosis	895:906	arg1	higher					1003:1008	higher	1003:1008	higher	1003:1008	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	3	66	theme	total	561:565	arg1	ration					573:578	the total mixed ration	557:578	the total mixed ration	557:578	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	5	67	theme	control	868:874	arg1	group					876:880	the control group	864:880	the control group	864:880	Each period consisted of an adaptation period of 12 d and a test period of 2 d. Dairy cows fed the diet supplemented with 5-ALA exhibited increased counts of white blood cells and granulocytes compared with the control group.					
31587902	6	68	from	cows	983:986	arg1	proliferation					928:940	mitogen-induced proliferation	912:940	mitogen-induced proliferation	912:940	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	6	68	from	cows	983:986	arg1	phagocytosis					895:906	phagocytosis	895:906	phagocytosis	895:906	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	4	69	theme	crossover	624:632	arg1	design					634:639	a crossover design	622:639	a crossover design	622:639	The experiments followed a crossover design with 2 periods.					
31587902	8	70	theme	casein	1176:1181	arg1	contents					1183:1190	milk casein contents	1171:1190	milk casein contents	1171:1190	Supplementation with 5-ALA improved milk protein and milk casein contents; however, it had no effect on milk production, milk fat, lactose, total solids, or solids-not-fat, compared with the control.					
31587902	0	71	theme	iron	69:72	arg1	status					74:79	iron status	69:79	iron status	69:79	Effects of 5-aminolevulinic acid supplementation on milk production, iron status, and immune response of dairy cows.					
31587902	3	72	theme	mixed	567:571	arg1	ration					573:578	the total mixed ration	557:578	the total mixed ration	557:578	During feeding, 5-ALA was mixed with a small amount of the total mixed ration and top-dressed.					
31587902	9	73	theme	immune	1453:1458	arg1	response					1460:1467	the immune response	1449:1467	the immune response	1449:1467	We conclude that dietary supplementation of 5-ALA to lactating dairy cows may have a positive effect on milk protein synthesis and the immune response.					
31587902	6	74	theme	mononuclear	962:972	arg1	cells					974:978	peripheral blood mononuclear cells	945:978	peripheral blood mononuclear cells in cows fed 5-ALA	945:996	The rate of phagocytosis and mitogen-induced proliferation of peripheral blood mononuclear cells in cows fed 5-ALA were higher than in cows fed a basal diet.					
31587902	2	75	theme	treatment	425:433	arg1	group					435:439	a treatment group	423:439	a treatment group supplemented with 10 mg of 5-ALA per kilogram of dry matter	423:499	In this study 13 lactating Holstein cows were randomly assigned to either a control group or a treatment group supplemented with 10 mg of 5-ALA per kilogram of dry matter.					
31587902	8	76	with	Supplementation	1118:1132	arg1	5-ALA					1139:1143	5-ALA	1139:1143	5-ALA	1139:1143	Supplementation with 5-ALA improved milk protein and milk casein contents; however, it had no effect on milk production, milk fat, lactose, total solids, or solids-not-fat, compared with the control.					
31587902	8	77	theme	milk	1154:1157	arg1	protein					1159:1165	milk protein	1154:1165	milk protein	1154:1165	Supplementation with 5-ALA improved milk protein and milk casein contents; however, it had no effect on milk production, milk fat, lactose, total solids, or solids-not-fat, compared with the control.					
30769335	9	0	theme	3D	1680:1681	arg1	studies					1702:1708	sophisticated 3D cell culture model studies	1666:1708	sophisticated 3D cell culture model studies	1666:1708	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	1	1	theme	Calcific	147:154	arg1	disease					169:175	INTRODUCTION Calcific aortic valve disease	134:175	INTRODUCTION Calcific aortic valve disease (CAVD)	134:182	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	1	theme	Calcific	147:154	arg1	disease					224:230	the most common acquired heart valve disease	187:230	the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood	187:304	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	1	theme	Calcific	147:154	arg1	CAVD					178:181	CAVD	178:181	CAVD	178:181	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	8	2	dep	cell	1508:1511	arg1	proliferation					1524:1536	proliferation	1524:1536	proliferation	1524:1536	No cytotoxic effects were detected and AVdH showed positive effects on cell growth and proliferation in vitro when compared to Collagen type I and Matrigel®.					
30769335	8	2	dep	cell	1508:1511	arg1	growth					1513:1518	growth	1513:1518	growth	1513:1518	No cytotoxic effects were detected and AVdH showed positive effects on cell growth and proliferation in vitro when compared to Collagen type I and Matrigel®.					
30769335	9	3	theme	culture	1688:1694	arg1	studies					1702:1708	sophisticated 3D cell culture model studies	1666:1708	sophisticated 3D cell culture model studies	1666:1708	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	6	4	theme	native	1157:1162	arg1	proteins					1164:1171	native proteins	1157:1171	native proteins	1157:1171	RESULTS On the biochemical level, a complex composition of native proteins was detected.					
30769335	7	5	theme	binding	1419:1425	arg1	activity					1427:1434	binding activity	1419:1434	binding activity	1419:1434	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	4	6	theme	detailed	668:675	arg1	analysis					677:684	A detailed analysis	666:684	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments	666:824	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	7	7	with	analysis	1285:1292	arg1	network					1262:1268	Gene Ontology network	1248:1268	Gene Ontology network	1248:1268	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	2	8	theme	Three-dimensional	307:323	arg1	models					343:348	Three-dimensional (3D) cell culture models	307:348	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques	307:407	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	2	8	theme	Three-dimensional	307:323	arg1	platform					468:475	a transitional platform	453:475	a transitional platform between conventional 2D cell culture and in vivo experiments	453:536	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	1	9	theme	acquired	203:210	arg1	disease					169:175	INTRODUCTION Calcific aortic valve disease	134:175	INTRODUCTION Calcific aortic valve disease (CAVD)	134:182	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	9	theme	acquired	203:210	arg1	disease					224:230	the most common acquired heart valve disease	187:230	the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood	187:304	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	5	10	theme	type	1076:1079	arg1	I					1081:1081	Collagen type I	1067:1081	Collagen type I	1067:1081	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	1	11	theme	valve	218:222	arg1	disease					169:175	INTRODUCTION Calcific aortic valve disease	134:175	INTRODUCTION Calcific aortic valve disease (CAVD)	134:182	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	11	theme	valve	218:222	arg1	disease					224:230	the most common acquired heart valve disease	187:230	the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood	187:304	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	4	12	theme	rheological	718:728	arg1	composition					705:715	protein composition	697:715	protein composition	697:715	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	4	12	theme	rheological	718:728	arg1	behavior					730:737	rheological behavior	718:737	rheological behavior	718:737	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	9	13	dep	in	1802:1803	arg1	vitro					1805:1809	vitro	1805:1809	vitro	1805:1809	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	5	14	theme	used	1042:1045	arg1	solutions					1051:1059	other currently used ECM solutions	1026:1059	other currently used ECM solutions	1026:1059	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	1	15	theme	INTRODUCTION	134:145	arg1	disease					169:175	INTRODUCTION Calcific aortic valve disease	134:175	INTRODUCTION Calcific aortic valve disease (CAVD)	134:182	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	15	theme	INTRODUCTION	134:145	arg1	disease					224:230	the most common acquired heart valve disease	187:230	the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood	187:304	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	15	theme	INTRODUCTION	134:145	arg1	CAVD					178:181	CAVD	178:181	CAVD	178:181	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	3	16	attach	derived	621:627	arg2	hydrogel					612:619	a novel 3D hydrogel	601:619	a novel 3D hydrogel derived from cell-free native aortic valves	601:663	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	3	16	attach	derived	621:627	arg1	valves					658:663	cell-free native aortic valves	634:663	cell-free native aortic valves	634:663	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	5	17	theme	biological	931:940	arg1	components					969:978	commercially available biological extracellular matrix (ECM) components	908:978	commercially available biological extracellular matrix (ECM) components	908:978	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	3	18	dep	METHODS	539:545	arg1	report					555:560	report	555:560	report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves	555:663	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	2	19	theme	cell	501:504	arg1	culture					506:512	conventional 2D cell culture	485:512	conventional 2D cell culture	485:512	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	7	20	theme	mass	1225:1228	arg1	spectrometry					1230:1241	mass spectrometry	1225:1241	mass spectrometry with Gene Ontology network	1225:1268	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	3	21	theme	3D	609:610	arg1	hydrogel					612:619	a novel 3D hydrogel	601:619	a novel 3D hydrogel derived from cell-free native aortic valves	601:663	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	2	22	theme	conventional	485:496	arg1	culture					506:512	conventional 2D cell culture	485:512	conventional 2D cell culture	485:512	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	0	23	theme	culture	80:86	arg1	analyses					88:95	three dimensional culture analyses	62:95	three dimensional culture analyses	62:95	Native aortic valve derived extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties.					
30769335	4	24	theme	proliferative	754:766	arg1	effects					768:774	cytotoxic and proliferative effects	740:774	cytotoxic and proliferative effects	740:774	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	4	24	theme	proliferative	754:766	arg1	composition					705:715	protein composition	697:715	protein composition	697:715	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	7	25	theme	different	1193:1201	arg1	spectrometry					1230:1241	mass spectrometry	1225:1241	mass spectrometry with Gene Ontology network	1225:1268	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	7	25	theme	different	1193:1201	arg1	techniques					1203:1212	different techniques	1193:1212	different techniques	1193:1212	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	7	25	theme	different	1193:1201	arg1	analysis					1285:1292	enrichment analysis	1274:1292	enrichment analysis	1274:1292	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	1	26	theme	aortic	156:161	arg1	disease					169:175	INTRODUCTION Calcific aortic valve disease	134:175	INTRODUCTION Calcific aortic valve disease (CAVD)	134:182	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	26	theme	aortic	156:161	arg1	disease					224:230	the most common acquired heart valve disease	187:230	the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood	187:304	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	26	theme	aortic	156:161	arg1	CAVD					178:181	CAVD	178:181	CAVD	178:181	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	9	27	theme	enhanced	1793:1800	arg1	analyses					1811:1818	enhanced in vitro analyses	1793:1818	enhanced in vitro analyses	1793:1818	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	2	28	dep	two-dimensional	377:391	arg1	2D					394:395	2D	394:395	2D	394:395	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	0	29	theme	biomimetic	111:120	arg1	properties					122:131	improved biomimetic properties	102:131	improved biomimetic properties	102:131	Native aortic valve derived extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties.					
30769335	9	30	theme	valve	1764:1768	arg1	environment					1738:1748	the native environment	1727:1748	the native environment of the aortic valve	1727:1768	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	0	31	theme	Native	0:5	arg1	valve					14:18	Native aortic valve	0:18	Native aortic valve	0:18	Native aortic valve derived extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties.					
30769335	5	32	theme	matrix	956:961	arg1	components					969:978	commercially available biological extracellular matrix (ECM) components	908:978	commercially available biological extracellular matrix (ECM) components	908:978	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	2	33	theme	CAVD	435:438	arg1	aspects					424:430	new aspects	420:430	new aspects of CAVD	420:438	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	5	34	theme	ECM	964:966	arg1	components					969:978	commercially available biological extracellular matrix (ECM) components	908:978	commercially available biological extracellular matrix (ECM) components	908:978	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	7	35	theme	biological	1317:1326	arg1	functions					1328:1336	different fundamental biological functions	1295:1336	different fundamental biological functions of AVdH	1295:1344	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	3	36	theme	aortic	651:656	arg1	valves					658:663	cell-free native aortic valves	634:663	cell-free native aortic valves	634:663	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	0	37	theme	extracellular	28:40	arg1	hydrogel					49:56	extracellular matrix hydrogel	28:56	extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties	28:131	Native aortic valve derived extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties.					
30769335	2	38	dep	in	518:519	arg1	vivo					521:524	vivo	521:524	vivo	521:524	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	7	39	theme	different	1295:1303	arg1	functions					1328:1336	different fundamental biological functions	1295:1336	different fundamental biological functions of AVdH	1295:1344	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	3	40	theme	cell-free	634:642	arg1	valves					658:663	cell-free native aortic valves	634:663	cell-free native aortic valves	634:663	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	6	41	theme	biochemical	1113:1123	arg1	level					1125:1129	the biochemical level	1109:1129	the biochemical level	1109:1129	RESULTS On the biochemical level, a complex composition of native proteins was detected.					
30769335	4	42	theme	3D	798:799	arg1	experiments					814:824	3D cell culture experiments	798:824	3D cell culture experiments	798:824	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	2	43	theme	culture	335:341	arg1	models					343:348	Three-dimensional (3D) cell culture models	307:348	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques	307:407	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	2	43	theme	culture	335:341	arg1	platform					468:475	a transitional platform	453:475	a transitional platform between conventional 2D cell culture and in vivo experiments	453:536	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	5	44	dep	I	1081:1081	arg1	i.e.					1062:1065	i.e.	1062:1065	i.e.	1062:1065	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	4	45	theme	culture	806:812	arg1	experiments					814:824	3D cell culture experiments	798:824	3D cell culture experiments	798:824	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	0	46	with	hydrogel	49:56	arg1	properties					122:131	improved biomimetic properties	102:131	improved biomimetic properties	102:131	Native aortic valve derived extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties.					
30769335	2	47	theme	two-dimensional	377:391	arg1	techniques					398:407	conventional two-dimensional (2D) techniques	364:407	conventional two-dimensional (2D) techniques	364:407	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	1	48	with	disease	224:230	arg1	complex					237:243	complex	237:243	complex underlying pathomechanisms that are yet not fully understood	237:304	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	7	49	theme	Ontology	1253:1260	arg1	network					1262:1268	Gene Ontology network	1248:1268	Gene Ontology network	1248:1268	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	7	50	with	spectrometry	1230:1241	arg1	network					1262:1268	Gene Ontology network	1248:1268	Gene Ontology network	1248:1268	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	9	51	theme	sophisticated	1666:1678	arg1	studies					1702:1708	sophisticated 3D cell culture model studies	1666:1708	sophisticated 3D cell culture model studies	1666:1708	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	4	52	contain	containing	686:695	arg2	effects					768:774	cytotoxic and proliferative effects	740:774	cytotoxic and proliferative effects	740:774	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	4	52	contain	containing	686:695	arg1	analysis					677:684	A detailed analysis	666:684	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments	666:824	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	4	52	contain	containing	686:695	arg2	behavior					730:737	rheological behavior	718:737	rheological behavior	718:737	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	4	52	contain	containing	686:695	arg2	composition					705:715	protein composition	697:715	protein composition	697:715	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	9	53	theme	cell	1683:1686	arg1	studies					1702:1708	sophisticated 3D cell culture model studies	1666:1708	sophisticated 3D cell culture model studies	1666:1708	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	6	54	theme	proteins	1164:1171	arg1	composition					1142:1152	a complex composition	1132:1152	a complex composition of native proteins	1132:1171	RESULTS On the biochemical level, a complex composition of native proteins was detected.					
30769335	9	55	theme	model	1696:1700	arg1	studies					1702:1708	sophisticated 3D cell culture model studies	1666:1708	sophisticated 3D cell culture model studies	1666:1708	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	6	56	theme	complex	1134:1140	arg1	composition					1142:1152	a complex composition	1132:1152	a complex composition of native proteins	1132:1171	RESULTS On the biochemical level, a complex composition of native proteins was detected.					
30769335	1	57	theme	common	196:201	arg1	disease					169:175	INTRODUCTION Calcific aortic valve disease	134:175	INTRODUCTION Calcific aortic valve disease (CAVD)	134:182	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	57	theme	common	196:201	arg1	disease					224:230	the most common acquired heart valve disease	187:230	the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood	187:304	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	5	58	theme	Collagen	1067:1074	arg1	I					1081:1081	Collagen type I	1067:1081	Collagen type I	1067:1081	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	1	59	theme	heart	212:216	arg1	disease					169:175	INTRODUCTION Calcific aortic valve disease	134:175	INTRODUCTION Calcific aortic valve disease (CAVD)	134:182	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	59	theme	heart	212:216	arg1	disease					224:230	the most common acquired heart valve disease	187:230	the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood	187:304	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	9	60	theme	native	1731:1736	arg1	environment					1738:1748	the native environment	1727:1748	the native environment of the aortic valve	1727:1768	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	8	61	theme	positive	1488:1495	arg1	effects					1497:1503	positive effects	1488:1503	positive effects on cell growth and proliferation	1488:1536	No cytotoxic effects were detected and AVdH showed positive effects on cell growth and proliferation in vitro when compared to Collagen type I and Matrigel®.					
30769335	7	62	theme	peptidase	1385:1393	arg1	inhibitor-					1395:1404	peptidase inhibitor-	1385:1404	peptidase inhibitor-	1385:1404	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	2	63	dep	Three-dimensional	307:323	arg1	3D					326:327	3D	326:327	3D	326:327	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	5	64	theme	ECM	1047:1049	arg1	solutions					1051:1059	other currently used ECM solutions	1026:1059	other currently used ECM solutions	1026:1059	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	3	65	theme	hydrogel	612:619	arg1	characterization					581:596	characterization	581:596	characterization	581:596	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	3	65	theme	hydrogel	612:619	arg1	fabrication					565:575	fabrication	565:575	fabrication	565:575	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	5	66	theme	available	921:929	arg1	components					969:978	commercially available biological extracellular matrix (ECM) components	908:978	commercially available biological extracellular matrix (ECM) components	908:978	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	9	67	theme	aortic	1757:1762	arg1	valve					1764:1768	the aortic valve	1753:1768	the aortic valve	1753:1768	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	5	68	dep	components	969:978	arg1	is					893:894	is	893:894	is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®	893:1095	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	0	69	theme	dimensional	68:78	arg1	analyses					88:95	three dimensional culture analyses	62:95	three dimensional culture analyses	62:95	Native aortic valve derived extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties.					
30769335	3	70	theme	novel	603:607	arg1	hydrogel					612:619	a novel 3D hydrogel	601:619	a novel 3D hydrogel derived from cell-free native aortic valves	601:663	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	1	71	theme	valve	163:167	arg1	disease					169:175	INTRODUCTION Calcific aortic valve disease	134:175	INTRODUCTION Calcific aortic valve disease (CAVD)	134:182	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	71	theme	valve	163:167	arg1	disease					224:230	the most common acquired heart valve disease	187:230	the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood	187:304	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	1	71	theme	valve	163:167	arg1	CAVD					178:181	CAVD	178:181	CAVD	178:181	INTRODUCTION Calcific aortic valve disease (CAVD) is the most common acquired heart valve disease with complex underlying pathomechanisms that are yet not fully understood.					
30769335	2	72	theme	2D	498:499	arg1	culture					506:512	conventional 2D cell culture	485:512	conventional 2D cell culture	485:512	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	9	73	dep	CONCLUSION	1595:1604	arg1	suggest					1620:1626	suggest	1620:1626	suggest	1620:1626	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	8	74	from	effects	1497:1503	arg1	cell					1508:1511	cell growth and proliferation	1508:1536	cell growth and proliferation	1508:1536	No cytotoxic effects were detected and AVdH showed positive effects on cell growth and proliferation in vitro when compared to Collagen type I and Matrigel®.					
30769335	9	75	theme	greater	1775:1781	arg1	level					1783:1787	a greater level	1773:1787	a greater level for enhanced in vitro analyses	1773:1818	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	4	76	theme	protein	697:703	arg1	composition					705:715	protein composition	697:715	protein composition	697:715	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	4	76	theme	protein	697:703	arg1	behavior					730:737	rheological behavior	718:737	rheological behavior	718:737	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	4	76	theme	protein	697:703	arg1	effects					768:774	cytotoxic and proliferative effects	740:774	cytotoxic and proliferative effects	740:774	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	0	77	theme	improved	102:109	arg1	properties					122:131	improved biomimetic properties	102:131	improved biomimetic properties	102:131	Native aortic valve derived extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties.					
30769335	2	78	theme	in	518:519	arg1	experiments					526:536	in vivo experiments	518:536	in vivo experiments	518:536	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	7	79	theme	AVdH	1341:1344	arg1	functions					1328:1336	different fundamental biological functions	1295:1336	different fundamental biological functions of AVdH	1295:1344	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	9	80	theme	in	1802:1803	arg1	analyses					1811:1818	enhanced in vitro analyses	1793:1818	enhanced in vitro analyses	1793:1818	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	0	81	theme	aortic	7:12	arg1	valve					14:18	Native aortic valve	0:18	Native aortic valve	0:18	Native aortic valve derived extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties.					
30769335	5	82	theme	extracellular	942:954	arg1	components					969:978	commercially available biological extracellular matrix (ECM) components	908:978	commercially available biological extracellular matrix (ECM) components	908:978	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	8	83	theme	Collagen	1564:1571	arg1	type					1573:1576	Collagen type I	1564:1578	Collagen type I	1564:1578	No cytotoxic effects were detected and AVdH showed positive effects on cell growth and proliferation in vitro when compared to Collagen type I and Matrigel®.					
30769335	4	84	theme	cytotoxic	740:748	arg1	effects					768:774	cytotoxic and proliferative effects	740:774	cytotoxic and proliferative effects	740:774	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	4	84	theme	cytotoxic	740:748	arg1	composition					705:715	protein composition	697:715	protein composition	697:715	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	7	85	theme	fundamental	1305:1315	arg1	functions					1328:1336	different fundamental biological functions	1295:1336	different fundamental biological functions of AVdH	1295:1344	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	3	86	theme	native	644:649	arg1	valves					658:663	cell-free native aortic valves	634:663	cell-free native aortic valves	634:663	METHODS Here we report on fabrication and characterization of a novel 3D hydrogel derived from cell-free native aortic valves.					
30769335	2	87	dep	models	343:348	arg1	opposed					353:359	opposed	353:359	opposed to conventional two-dimensional (2D) techniques	353:407	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	2	88	theme	new	420:422	arg1	aspects					424:430	new aspects	420:430	new aspects of CAVD	420:438	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	0	89	theme	matrix	42:47	arg1	hydrogel					49:56	extracellular matrix hydrogel	28:56	extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties	28:131	Native aortic valve derived extracellular matrix hydrogel for three dimensional culture analyses with improved biomimetic properties.					
30769335	2	90	theme	transitional	455:466	arg1	models					343:348	Three-dimensional (3D) cell culture models	307:348	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques	307:407	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	2	90	theme	transitional	455:466	arg1	platform					468:475	a transitional platform	453:475	a transitional platform between conventional 2D cell culture and in vivo experiments	453:536	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	4	91	theme	experiments	814:824	arg1	analysis					677:684	A detailed analysis	666:684	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments	666:824	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	4	91	theme	experiments	814:824	arg1	results					787:793	results	787:793	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments	666:824	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	5	92	theme	other	1026:1030	arg1	solutions					1051:1059	other currently used ECM solutions	1026:1059	other currently used ECM solutions	1026:1059	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	7	93	theme	enrichment	1274:1283	arg1	analysis					1285:1292	enrichment analysis	1274:1292	enrichment analysis	1274:1292	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	8	94	theme	cytotoxic	1440:1448	arg1	effects					1450:1456	No cytotoxic effects	1437:1456	No cytotoxic effects	1437:1456	No cytotoxic effects were detected and AVdH showed positive effects on cell growth and proliferation in vitro when compared to Collagen type I and Matrigel®.					
30769335	2	95	theme	cell	330:333	arg1	models					343:348	Three-dimensional (3D) cell culture models	307:348	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques	307:407	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	2	95	theme	cell	330:333	arg1	platform					468:475	a transitional platform	453:475	a transitional platform between conventional 2D cell culture and in vivo experiments	453:536	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
30769335	5	96	theme	aortic	856:861	arg1	valve					863:867	this aortic valve	851:867	this aortic valve derived hydrogel (AVdH)	851:891	Moreover, this aortic valve derived hydrogel (AVdH) is compared to commercially available biological extracellular matrix (ECM) components to evaluate and classify AVdH with respect to other currently used ECM solutions, i.e. Collagen type I and Matrigel®.					
30769335	9	97	theme	organotypic	1639:1649	arg1	AVdH					1628:1631	AVdH	1628:1631	AVdH	1628:1631	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	9	97	theme	organotypic	1639:1649	arg1	ECM					1651:1653	an organotypic ECM	1636:1653	an organotypic ECM supporting sophisticated 3D cell culture model studies	1636:1708	CONCLUSION These results suggest AVdH as an organotypic ECM supporting sophisticated 3D cell culture model studies, while mimicking the native environment of the aortic valve to a greater level for enhanced in vitro analyses.					
30769335	7	98	theme	Gene	1248:1251	arg1	Ontology					1253:1260	Gene Ontology	1248:1260	Gene Ontology network	1248:1268	Using different techniques, including mass spectrometry with Gene Ontology network and enrichment analysis, different fundamental biological functions of AVdH were identified, including peptidase-, peptidase inhibitor-, growth- and binding activity.					
30769335	4	99	theme	cell	801:804	arg1	experiments					814:824	3D cell culture experiments	798:824	3D cell culture experiments	798:824	A detailed analysis containing protein composition, rheological behavior, cytotoxic and proliferative effects as well as results of 3D cell culture experiments are presented.					
30769335	6	100	from	RESULTS	1098:1104	arg1	level					1125:1129	the biochemical level	1109:1129	the biochemical level	1109:1129	RESULTS On the biochemical level, a complex composition of native proteins was detected.					
30769335	2	101	theme	conventional	364:375	arg1	techniques					398:407	conventional two-dimensional (2D) techniques	364:407	conventional two-dimensional (2D) techniques	364:407	Three-dimensional (3D) cell culture models as opposed to conventional two-dimensional (2D) techniques may reveal new aspects of CAVD and serve as a transitional platform between conventional 2D cell culture and in vivo experiments.					
29872294	8	0	theme	chemotherapy	1512:1523	arg1	combination					1497:1507	the combination	1493:1507	the combination of chemotherapy and photothermal therapy	1493:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	8	1	theme	promising	1451:1459	arg1	composite					1426:1434	The MWNTs-Ge-Le three-component anticancer composite	1383:1434	The MWNTs-Ge-Le three-component anticancer composite	1383:1434	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	8	1	theme	promising	1451:1459	arg1	candidate					1461:1469	a promising candidate	1449:1469	a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy	1449:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	1	2	theme	short	184:188	arg1	half-life					197:205	its short plasma half-life	180:205	its short plasma half-life	180:205	PURPOSE Gemcitabine's clinical application is limited due to its short plasma half-life and poor uptake by cells.					
29872294	0	3	theme	cancer	111:116	arg1	therapy					100:106	chemo-photothermal synergistic therapy	69:106	chemo-photothermal synergistic therapy of cancer	69:116	A carbon nanotube-gemcitabine-lentinan three-component composite for chemo-photothermal synergistic therapy of cancer.					
29872294	8	4	theme	therapy	1542:1548	arg1	combination					1497:1507	the combination	1493:1507	the combination of chemotherapy and photothermal therapy	1493:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	1	5	theme	plasma	190:195	arg1	half-life					197:205	its short plasma half-life	180:205	its short plasma half-life	180:205	PURPOSE Gemcitabine's clinical application is limited due to its short plasma half-life and poor uptake by cells.					
29872294	8	6	theme	photothermal	1529:1540	arg1	therapy					1542:1548	photothermal therapy	1529:1548	photothermal therapy	1529:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	5	7	theme	photothermal	957:968	arg1	responses					970:978	the photothermal responses	953:978	the photothermal responses	953:978	Using the composite and an 808 nm laser, we treated tumors, both in vitro and in vivo, and investigated the photothermal responses and the anticancer efficacy.					
29872294	4	8	with	lentinan	576:583	arg1	MWNTs					590:594	MWNTs	590:594	MWNTs	590:594	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	2	9	theme	carbon	313:318	arg1	composite					290:298	a drug delivery three-component composite	258:298	a drug delivery three-component composite	258:298	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	9	theme	carbon	313:318	arg1	Le					365:366	Le	365:366	Le; MWNTs-Ge-Le	365:379	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	9	theme	carbon	313:318	arg1	/lentinan					354:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan	301:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le)	301:380	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	10	theme	nanotubes	320:328	arg1	composite					290:298	a drug delivery three-component composite	258:298	a drug delivery three-component composite	258:298	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	10	theme	nanotubes	320:328	arg1	Le					365:366	Le	365:366	Le; MWNTs-Ge-Le	365:379	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	10	theme	nanotubes	320:328	arg1	/lentinan					354:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan	301:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le)	301:380	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	4	11	dep	conjugated	549:558	arg1	METHODS					523:529	METHODS	523:529	METHODS In this study	523:543	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	7	12	theme	808	1223:1225	arg1	nm					1227:1228	nm	1227:1228	nm	1227:1228	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	2	13	theme	multiwalled	301:311	arg1	composite					290:298	a drug delivery three-component composite	258:298	a drug delivery three-component composite	258:298	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	13	theme	multiwalled	301:311	arg1	Le					365:366	Le	365:366	Le; MWNTs-Ge-Le	365:379	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	13	theme	multiwalled	301:311	arg1	/lentinan					354:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan	301:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le)	301:380	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	3	14	theme	therapy	471:477	arg1	combination					426:436	the combination	422:436	the combination of chemotherapy and photothermal therapy	422:477	Moreover, the combination of chemotherapy and photothermal therapy was employed to enhance antitumor efficacy.					
29872294	4	15	theme	chemical	668:675	arg1	structure					677:685	the chemical structure	664:685	the chemical structure of MWNTs-Ge-Le	664:700	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	5	16	theme	nm	880:881	arg1	laser					883:887	the composite and an 808 nm laser	855:887	laser	883:887	Using the composite and an 808 nm laser, we treated tumors, both in vitro and in vivo, and investigated the photothermal responses and the anticancer efficacy.					
29872294	7	17	theme	therapy	1316:1322	arg1	combination					1257:1267	the combination	1253:1267	the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy	1253:1369	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	2	18	dep	Le	365:366	arg1	MWNTs-Ge-Le					369:379	MWNTs-Ge-Le	369:379	Le; MWNTs-Ge-Le	365:379	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	5	19	theme	808	876:878	arg1	nm					880:881	nm	880:881	nm	880:881	Using the composite and an 808 nm laser, we treated tumors, both in vitro and in vivo, and investigated the photothermal responses and the anticancer efficacy.					
29872294	6	20	dep	RESULTS	1009:1015	arg1	cross					1061:1065	cross	1061:1065	cross	1061:1065	RESULTS The MWNTs-Ge-Le composite could efficiently cross cell membrane, having a higher antitumor activity than MWNTs, gemcitabine and MWNTs-Ge in vitro and in vivo.					
29872294	7	21	theme	MWNTs-Ge-Le	1193:1203	arg1	composite					1205:1213	the MWNTs-Ge-Le composite	1189:1213	the MWNTs-Ge-Le composite with an 808 nm laser radiation	1189:1244	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	7	22	theme	laser	1230:1234	arg1	radiation					1236:1244	an 808 nm laser radiation	1220:1244	an 808 nm laser radiation	1220:1244	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	1	23	theme	PURPOSE	119:125	arg1	application					150:160	PURPOSE Gemcitabine's clinical application	119:160	PURPOSE Gemcitabine's clinical application	119:160	PURPOSE Gemcitabine's clinical application is limited due to its short plasma half-life and poor uptake by cells.					
29872294	1	24	theme	poor	211:214	arg1	uptake					216:221	poor uptake	211:221	poor uptake by cells	211:230	PURPOSE Gemcitabine's clinical application is limited due to its short plasma half-life and poor uptake by cells.					
29872294	7	25	with	composite	1205:1213	arg1	radiation					1236:1244	an 808 nm laser radiation	1220:1244	an 808 nm laser radiation	1220:1244	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	2	26	theme	MWNTs	331:335	arg1	composite					290:298	a drug delivery three-component composite	258:298	a drug delivery three-component composite	258:298	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	26	theme	MWNTs	331:335	arg1	Le					365:366	Le	365:366	Le; MWNTs-Ge-Le	365:379	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	26	theme	MWNTs	331:335	arg1	/lentinan					354:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan	301:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le)	301:380	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	7	27	theme	photothermal	1303:1314	arg1	therapy					1316:1322	near-infrared photothermal therapy	1289:1322	near-infrared photothermal therapy	1289:1322	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	0	28	theme	three-component	39:53	arg1	composite					55:63	three-component composite	39:63	three-component composite for chemo-photothermal synergistic therapy of cancer	39:116	A carbon nanotube-gemcitabine-lentinan three-component composite for chemo-photothermal synergistic therapy of cancer.					
29872294	7	29	theme	great	1334:1338	arg1	efficacy					1362:1369	great synergistic antitumor efficacy	1334:1369	great synergistic antitumor efficacy	1334:1369	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	7	30	theme	synergistic	1340:1350	arg1	efficacy					1362:1369	great synergistic antitumor efficacy	1334:1369	great synergistic antitumor efficacy	1334:1369	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	8	31	theme	MWNTs-Ge-Le	1387:1397	arg1	composite					1426:1434	The MWNTs-Ge-Le three-component anticancer composite	1383:1434	The MWNTs-Ge-Le three-component anticancer composite	1383:1434	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	8	31	theme	MWNTs-Ge-Le	1387:1397	arg1	candidate					1461:1469	a promising candidate	1449:1469	a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy	1449:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	8	32	theme	anticancer	1415:1424	arg1	composite					1426:1434	The MWNTs-Ge-Le three-component anticancer composite	1383:1434	The MWNTs-Ge-Le three-component anticancer composite	1383:1434	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	8	32	theme	anticancer	1415:1424	arg1	candidate					1461:1469	a promising candidate	1449:1469	a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy	1449:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	3	33	theme	chemotherapy	441:452	arg1	combination					426:436	the combination	422:436	the combination of chemotherapy and photothermal therapy	422:477	Moreover, the combination of chemotherapy and photothermal therapy was employed to enhance antitumor efficacy.					
29872294	1	34	theme	clinical	141:148	arg1	application					150:160	PURPOSE Gemcitabine's clinical application	119:160	PURPOSE Gemcitabine's clinical application	119:160	PURPOSE Gemcitabine's clinical application is limited due to its short plasma half-life and poor uptake by cells.					
29872294	6	35	theme	antitumor	1098:1106	arg1	activity					1108:1115	a higher antitumor activity	1089:1115	a higher antitumor activity than MWNTs, gemcitabine and MWNTs-Ge in vitro and in vivo	1089:1173	RESULTS The MWNTs-Ge-Le composite could efficiently cross cell membrane, having a higher antitumor activity than MWNTs, gemcitabine and MWNTs-Ge in vitro and in vivo.					
29872294	7	36	theme	drug	1272:1275	arg1	therapy					1277:1283	drug therapy	1272:1283	drug therapy	1272:1283	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	4	37	dep	transform	731:739	arg1	infrared					741:748	infrared	741:748	transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy	731:846	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	7	38	theme	nm	1227:1228	arg1	radiation					1236:1244	an 808 nm laser radiation	1220:1244	an 808 nm laser radiation	1220:1244	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	4	39	theme	electron	828:835	arg1	microscopy					837:846	transmission electron microscopy	815:846	transmission electron microscopy	815:846	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	7	40	from	study	1180:1184	arg1	composite					1205:1213	the MWNTs-Ge-Le composite	1189:1213	the MWNTs-Ge-Le composite with an 808 nm laser radiation	1189:1244	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	4	41	theme	transmission	815:826	arg1	microscopy					837:846	transmission electron microscopy	815:846	transmission electron microscopy	815:846	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	6	42	theme	cell	1067:1070	arg1	membrane					1072:1079	cell membrane	1067:1079	cell membrane	1067:1079	RESULTS The MWNTs-Ge-Le composite could efficiently cross cell membrane, having a higher antitumor activity than MWNTs, gemcitabine and MWNTs-Ge in vitro and in vivo.					
29872294	0	43	theme	synergistic	88:98	arg1	therapy					100:106	chemo-photothermal synergistic therapy	69:106	chemo-photothermal synergistic therapy of cancer	69:116	A carbon nanotube-gemcitabine-lentinan three-component composite for chemo-photothermal synergistic therapy of cancer.					
29872294	8	44	theme	cancer	1475:1480	arg1	therapy					1482:1488	cancer therapy	1475:1488	cancer therapy in the combination of chemotherapy and photothermal therapy	1475:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	4	45	theme	MWNTs-Ge-Le	690:700	arg1	structure					677:685	the chemical structure	664:685	the chemical structure of MWNTs-Ge-Le	664:700	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	0	46	theme	chemo-photothermal	69:86	arg1	therapy					100:106	chemo-photothermal synergistic therapy	69:106	chemo-photothermal synergistic therapy of cancer	69:116	A carbon nanotube-gemcitabine-lentinan three-component composite for chemo-photothermal synergistic therapy of cancer.					
29872294	7	47	dep	combination	1257:1267	arg1	possesses					1324:1332	possesses	1324:1332	possesses great synergistic antitumor efficacy	1324:1369	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	2	48	theme	Ge	351:352	arg1	composite					290:298	a drug delivery three-component composite	258:298	a drug delivery three-component composite	258:298	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	48	theme	Ge	351:352	arg1	Le					365:366	Le	365:366	Le; MWNTs-Ge-Le	365:379	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	48	theme	Ge	351:352	arg1	/lentinan					354:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan	301:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le)	301:380	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	5	49	theme	anticancer	988:997	arg1	efficacy					999:1006	the anticancer efficacy	984:1006	the anticancer efficacy	984:1006	Using the composite and an 808 nm laser, we treated tumors, both in vitro and in vivo, and investigated the photothermal responses and the anticancer efficacy.					
29872294	4	50	theme	thermogravimetric	784:800	arg1	analysis					802:809	thermogravimetric analysis	784:809	thermogravimetric analysis	784:809	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	2	51	theme	three-component	274:288	arg1	composite					290:298	a drug delivery three-component composite	258:298	a drug delivery three-component composite	258:298	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	51	theme	three-component	274:288	arg1	/lentinan					354:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan	301:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le)	301:380	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	8	52	theme	three-component	1399:1413	arg1	composite					1426:1434	The MWNTs-Ge-Le three-component anticancer composite	1383:1434	The MWNTs-Ge-Le three-component anticancer composite	1383:1434	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	8	52	theme	three-component	1399:1413	arg1	candidate					1461:1469	a promising candidate	1449:1469	a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy	1449:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	3	53	theme	photothermal	458:469	arg1	therapy					471:477	photothermal therapy	458:477	photothermal therapy	458:477	Moreover, the combination of chemotherapy and photothermal therapy was employed to enhance antitumor efficacy.					
29872294	4	54	theme	Raman	764:768	arg1	spectroscopy					770:781	Raman spectroscopy	764:781	Raman spectroscopy	764:781	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	2	55	theme	delivery	265:272	arg1	composite					290:298	a drug delivery three-component composite	258:298	a drug delivery three-component composite	258:298	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	55	theme	delivery	265:272	arg1	/lentinan					354:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan	301:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le)	301:380	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	56	theme	/gemcitabine	337:348	arg1	composite					290:298	a drug delivery three-component composite	258:298	a drug delivery three-component composite	258:298	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	56	theme	/gemcitabine	337:348	arg1	Le					365:366	Le	365:366	Le; MWNTs-Ge-Le	365:379	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	56	theme	/gemcitabine	337:348	arg1	/lentinan					354:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan	301:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le)	301:380	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	7	57	theme	near-infrared	1289:1301	arg1	therapy					1316:1322	near-infrared photothermal therapy	1289:1322	near-infrared photothermal therapy	1289:1322	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	4	58	dep	Fourier	723:729	arg1	transform					731:739	transform	731:739	transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy	731:846	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	8	59	from	therapy	1482:1488	arg1	combination					1497:1507	the combination	1493:1507	the combination of chemotherapy and photothermal therapy	1493:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
29872294	6	60	theme	MWNTs-Ge-Le	1021:1031	arg1	composite					1033:1041	The MWNTs-Ge-Le composite	1017:1041	The MWNTs-Ge-Le composite	1017:1041	RESULTS The MWNTs-Ge-Le composite could efficiently cross cell membrane, having a higher antitumor activity than MWNTs, gemcitabine and MWNTs-Ge in vitro and in vivo.					
29872294	2	61	theme	drug	260:263	arg1	composite					290:298	a drug delivery three-component composite	258:298	a drug delivery three-component composite	258:298	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	2	61	theme	drug	260:263	arg1	/lentinan					354:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan	301:362	multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le)	301:380	To address this problem, a drug delivery three-component composite, multiwalled carbon nanotubes (MWNTs)/gemcitabine (Ge)/lentinan (Le; MWNTs-Ge-Le), was fabricated in our study.					
29872294	4	62	theme	noncovalent	615:625	arg1	way					627:629	a covalent and noncovalent way to functionalize with MWNTs	600:657	a covalent and noncovalent way to functionalize with MWNTs	600:657	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	3	63	theme	antitumor	503:511	arg1	efficacy					513:520	antitumor efficacy	503:520	antitumor efficacy	503:520	Moreover, the combination of chemotherapy and photothermal therapy was employed to enhance antitumor efficacy.					
29872294	7	64	theme	therapy	1277:1283	arg1	combination					1257:1267	the combination	1253:1267	the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy	1253:1369	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	4	65	theme	covalent	602:609	arg1	way					627:629	a covalent and noncovalent way to functionalize with MWNTs	600:657	a covalent and noncovalent way to functionalize with MWNTs	600:657	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	4	66	from	METHODS	523:529	arg1	study					539:543	this study	534:543	this study	534:543	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	7	67	theme	antitumor	1352:1360	arg1	efficacy					1362:1369	great synergistic antitumor efficacy	1334:1369	great synergistic antitumor efficacy	1334:1369	Our study on the MWNTs-Ge-Le composite with an 808 nm laser radiation showed the combination of drug therapy and near-infrared photothermal therapy possesses great synergistic antitumor efficacy.					
29872294	4	68	with	gemcitabine	560:570	arg1	MWNTs					590:594	MWNTs	590:594	MWNTs	590:594	METHODS In this study, we conjugated gemcitabine and lentinan with MWNTs via a covalent and noncovalent way to functionalize with MWNTs, and the chemical structure of MWNTs-Ge-Le was characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, thermogravimetric analysis and transmission electron microscopy.					
29872294	6	69	theme	higher	1091:1096	arg1	activity					1108:1115	a higher antitumor activity	1089:1115	a higher antitumor activity than MWNTs, gemcitabine and MWNTs-Ge in vitro and in vivo	1089:1173	RESULTS The MWNTs-Ge-Le composite could efficiently cross cell membrane, having a higher antitumor activity than MWNTs, gemcitabine and MWNTs-Ge in vitro and in vivo.					
29872294	8	70	dep	CONCLUSION	1372:1381	arg1	serve					1440:1444	serve	1440:1444	can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy	1436:1548	CONCLUSION The MWNTs-Ge-Le three-component anticancer composite can serve as a promising candidate for cancer therapy in the combination of chemotherapy and photothermal therapy.					
31537749	8	0	theme	altered	1239:1245	arg1	composition					1257:1267	altered cell wall composition	1239:1267	altered cell wall composition	1239:1267	This suggests that the cytoskeleton plays an active role in compensating for altered cell wall composition.					
31537749	8	1	theme	cell	1247:1250	arg1	composition					1257:1267	altered cell wall composition	1239:1267	altered cell wall composition	1239:1267	This suggests that the cytoskeleton plays an active role in compensating for altered cell wall composition.					
31537749	1	2	theme	plant	146:150	arg1	organs					152:157	all aerial plant organs	135:157	all aerial plant organs	135:157	The shoot apical meristem (SAM) gives rise to all aerial plant organs.					
31537749	5	3	theme	stiffness	814:822	arg1	indicator					796:804	an indicator	793:804	an indicator of wall stiffness	793:822	No difference in the Young's modulus (i.e. an indicator of wall stiffness) of the cell walls was observed when XyGs were perturbed.					
31537749	2	4	theme	precise	322:328	arg1	role					330:333	their precise role	316:333	their precise role in morphogenesis during organ formation	316:373	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	6	5	theme	pectin	999:1004	arg1	status					1018:1023	pectin methylation status	999:1023	pectin methylation status	999:1023	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	5	6	dep	modulus	779:785	arg1	indicator					796:804	an indicator	793:804	an indicator of wall stiffness	793:822	No difference in the Young's modulus (i.e. an indicator of wall stiffness) of the cell walls was observed when XyGs were perturbed.					
31537749	5	7	theme	wall	809:812	arg1	stiffness					814:822	wall stiffness	809:822	wall stiffness	809:822	No difference in the Young's modulus (i.e. an indicator of wall stiffness) of the cell walls was observed when XyGs were perturbed.					
31537749	3	8	dep	major	453:457	arg1	characterized					484:496	characterized	484:496	characterized	484:496	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	3	9	theme	wall	499:502	arg1	xyloglucans					431:441	xyloglucans	431:441	xyloglucans (XyGs)	431:448	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	3	9	theme	wall	499:502	arg1	component					504:512	a major, yet functionally poorly characterized, wall component	451:512	a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana)	451:561	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	3	10	theme	major	453:457	arg1	xyloglucans					431:441	xyloglucans	431:441	xyloglucans (XyGs)	431:448	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	3	10	theme	major	453:457	arg1	component					504:512	a major, yet functionally poorly characterized, wall component	451:512	a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana)	451:561	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	5	11	theme	walls	837:841	arg1	modulus					779:785	the Young's modulus	767:785	the Young's modulus (i.e. an indicator of wall stiffness) of the cell walls	767:841	No difference in the Young's modulus (i.e. an indicator of wall stiffness) of the cell walls was observed when XyGs were perturbed.					
31537749	6	12	theme	wall	953:956	arg1	components					958:967	other cell wall components	942:967	other cell wall components such as cellulose content and pectin methylation status	942:1023	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	6	12	theme	wall	953:956	arg1	status					1018:1023	pectin methylation status	999:1023	pectin methylation status	999:1023	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	6	12	theme	wall	953:956	arg1	content					987:993	cellulose content	977:993	cellulose content	977:993	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	8	13	theme	wall	1252:1255	arg1	composition					1257:1267	altered cell wall composition	1239:1267	altered cell wall composition	1239:1267	This suggests that the cytoskeleton plays an active role in compensating for altered cell wall composition.					
31537749	4	14	theme	genetic	608:614	arg1	approaches					616:625	genetic approaches	608:625	genetic approaches	608:625	Using immunolabeling, biochemical analysis, genetic approaches, microindentation, laser ablation, and live imaging, we showed that XyGs are important for meristem shape and phyllotaxis.					
31537749	7	15	theme	microtubule	1061:1071	arg1	dynamics					1073:1080	cortical microtubule dynamics	1052:1080	cortical microtubule dynamics	1052:1080	Interestingly, control of cortical microtubule dynamics by the severing enzyme KATANIN became vital when XyGs were perturbed or absent.					
31537749	2	16	from	role	330:333	arg1	morphogenesis					338:350	morphogenesis	338:350	morphogenesis	338:350	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	1	17	theme	shoot	93:97	arg1	meristem					106:113	The shoot apical meristem	89:113	The shoot apical meristem (SAM)	89:119	The shoot apical meristem (SAM) gives rise to all aerial plant organs.					
31537749	1	17	theme	shoot	93:97	arg1	SAM					116:118	SAM	116:118	SAM	116:118	The shoot apical meristem (SAM) gives rise to all aerial plant organs.					
31537749	6	18	theme	cellulose	977:985	arg1	content					987:993	cellulose content	977:993	cellulose content	977:993	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	7	19	theme	severing	1089:1096	arg1	KATANIN					1105:1111	the severing enzyme KATANIN	1085:1111	the severing enzyme KATANIN	1085:1111	Interestingly, control of cortical microtubule dynamics by the severing enzyme KATANIN became vital when XyGs were perturbed or absent.					
31537749	1	20	theme	apical	99:104	arg1	meristem					106:113	The shoot apical meristem	89:113	The shoot apical meristem (SAM)	89:119	The shoot apical meristem (SAM) gives rise to all aerial plant organs.					
31537749	1	20	theme	apical	99:104	arg1	SAM					116:118	SAM	116:118	SAM	116:118	The shoot apical meristem (SAM) gives rise to all aerial plant organs.					
31537749	2	21	theme	organ	359:363	arg1	formation					365:373	organ formation	359:373	organ formation	359:373	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	7	22	theme	enzyme	1098:1103	arg1	KATANIN					1105:1111	the severing enzyme KATANIN	1085:1111	the severing enzyme KATANIN	1085:1111	Interestingly, control of cortical microtubule dynamics by the severing enzyme KATANIN became vital when XyGs were perturbed or absent.					
31537749	2	23	theme	central	193:199	arg1	role					201:204	a central role	191:204	a central role	191:204	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	2	24	theme	growth	280:285	arg1	rate					287:290	growth rate	280:290	growth rate	280:290	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	3	25	theme	xyloglucans	431:441	arg1	role					423:426	the role	419:426	the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana)	419:561	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	4	26	theme	laser	646:650	arg1	ablation					652:659	laser ablation	646:659	laser ablation	646:659	Using immunolabeling, biochemical analysis, genetic approaches, microindentation, laser ablation, and live imaging, we showed that XyGs are important for meristem shape and phyllotaxis.					
31537749	2	27	theme	dynamic	261:267	arg1	changes					269:275	dynamic changes	261:275	dynamic changes in growth rate and direction	261:304	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	5	28	theme	Young	771:775	arg1	modulus					779:785	the Young's modulus	767:785	the Young's modulus (i.e. an indicator of wall stiffness) of the cell walls	767:841	No difference in the Young's modulus (i.e. an indicator of wall stiffness) of the cell walls was observed when XyGs were perturbed.					
31537749	0	29	theme	Meristem	54:61	arg1	Geometry					63:70	Meristem Geometry	54:70	Meristem Geometry	54:70	Xyloglucans and Microtubules Synergistically Maintain Meristem Geometry and Phyllotaxis.					
31537749	6	30	from	Mutations	882:890	arg1	enzymes					895:901	enzymes	895:901	enzymes required for XyG synthesis	895:928	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	6	31	theme	cell	948:951	arg1	components					958:967	other cell wall components	942:967	other cell wall components such as cellulose content and pectin methylation status	942:1023	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	6	31	theme	cell	948:951	arg1	status					1018:1023	pectin methylation status	999:1023	pectin methylation status	999:1023	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	6	31	theme	cell	948:951	arg1	content					987:993	cellulose content	977:993	cellulose content	977:993	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	7	32	theme	dynamics	1073:1080	arg1	control					1041:1047	control	1041:1047	control of cortical microtubule dynamics by the severing enzyme KATANIN	1041:1111	Interestingly, control of cortical microtubule dynamics by the severing enzyme KATANIN became vital when XyGs were perturbed or absent.					
31537749	2	33	theme	Cell	160:163	arg1	walls					165:169	Cell walls	160:169	Cell walls	160:169	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	3	34	theme	Arabidopsis	541:551	arg1	thaliana					553:560	Arabidopsis (Arabidopsis thaliana)	528:561	Arabidopsis (Arabidopsis thaliana)	528:561	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	4	35	theme	biochemical	586:596	arg1	analysis					598:605	biochemical analysis	586:605	biochemical analysis	586:605	Using immunolabeling, biochemical analysis, genetic approaches, microindentation, laser ablation, and live imaging, we showed that XyGs are important for meristem shape and phyllotaxis.					
31537749	6	36	theme	other	942:946	arg1	components					958:967	other cell wall components	942:967	other cell wall components such as cellulose content and pectin methylation status	942:1023	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	6	36	theme	other	942:946	arg1	status					1018:1023	pectin methylation status	999:1023	pectin methylation status	999:1023	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	6	36	theme	other	942:946	arg1	content					987:993	cellulose content	977:993	cellulose content	977:993	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	8	37	theme	active	1207:1212	arg1	role					1214:1217	an active role	1204:1217	an active role	1204:1217	This suggests that the cytoskeleton plays an active role in compensating for altered cell wall composition.					
31537749	7	38	theme	cortical	1052:1059	arg1	dynamics					1073:1080	cortical microtubule dynamics	1052:1080	cortical microtubule dynamics	1052:1080	Interestingly, control of cortical microtubule dynamics by the severing enzyme KATANIN became vital when XyGs were perturbed or absent.					
31537749	3	39	theme	Arabidopsis	528:538	arg1	thaliana					553:560	Arabidopsis (Arabidopsis thaliana)	528:561	Arabidopsis (Arabidopsis thaliana)	528:561	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	3	40	from	component	504:512	arg1	SAM					521:523	the SAM	517:523	the SAM of Arabidopsis (Arabidopsis thaliana)	517:561	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	2	41	theme	molecular	235:243	arg1	regulation					245:254	molecular regulation	235:254	molecular regulation	235:254	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	5	42	dep	indicator	796:804	arg1	i.e.					788:791	i.e.	788:791	i.e.	788:791	No difference in the Young's modulus (i.e. an indicator of wall stiffness) of the cell walls was observed when XyGs were perturbed.					
31537749	5	43	theme	cell	832:835	arg1	walls					837:841	the cell walls	828:841	the cell walls	828:841	No difference in the Young's modulus (i.e. an indicator of wall stiffness) of the cell walls was observed when XyGs were perturbed.					
31537749	4	44	theme	meristem	718:725	arg1	shape					727:731	meristem shape	718:731	meristem shape	718:731	Using immunolabeling, biochemical analysis, genetic approaches, microindentation, laser ablation, and live imaging, we showed that XyGs are important for meristem shape and phyllotaxis.					
31537749	4	45	theme	live	666:669	arg1	imaging					671:677	live imaging	666:677	live imaging	666:677	Using immunolabeling, biochemical analysis, genetic approaches, microindentation, laser ablation, and live imaging, we showed that XyGs are important for meristem shape and phyllotaxis.					
31537749	2	46	from	changes	269:275	arg1	direction					296:304	direction	296:304	direction	296:304	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	2	46	from	changes	269:275	arg1	rate					287:290	growth rate	280:290	growth rate	280:290	Cell walls are thought to play a central role in this process, translating molecular regulation into dynamic changes in growth rate and direction, although their precise role in morphogenesis during organ formation is poorly understood.					
31537749	6	47	theme	methylation	1006:1016	arg1	status					1018:1023	pectin methylation status	999:1023	pectin methylation status	999:1023	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31537749	3	48	theme	thaliana	553:560	arg1	SAM					521:523	the SAM	517:523	the SAM of Arabidopsis (Arabidopsis thaliana)	517:561	Here, we investigated the role of xyloglucans (XyGs), a major, yet functionally poorly characterized, wall component in the SAM of Arabidopsis (Arabidopsis thaliana).					
31537749	1	49	theme	aerial	139:144	arg1	organs					152:157	all aerial plant organs	135:157	all aerial plant organs	135:157	The shoot apical meristem (SAM) gives rise to all aerial plant organs.					
31537749	6	50	theme	XyG	916:918	arg1	synthesis					920:928	XyG synthesis	916:928	XyG synthesis	916:928	Mutations in enzymes required for XyG synthesis also affect other cell wall components such as cellulose content and pectin methylation status.					
31869428	9	0	theme	rivaroxaban	1542:1552	arg1	mg					1557:1558	rivaroxaban 10 mg	1542:1558	rivaroxaban 10 mg for 10 days	1542:1570	Of those, 225 patients (28.4%) received fondaparinux 2.5 mg s.c. for 3 days, 166 patients (20.9%) received rivaroxaban 10 mg p.o. for 3 days and 402 patients (50.7%) received rivaroxaban 10 mg for 10 days.					
31869428	11	1	from	follow-up	1862:1870	arg1	endpoint					1843:1850	The cumulative primary composite endpoint	1810:1850	The cumulative primary composite endpoint at 4-week follow-up	1810:1870	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	1	from	follow-up	1862:1870	arg1	%					1879:1879	1.1%	1876:1879	1.1% (n = 9)	1876:1887	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	10	2	theme	2&ndash	1601:1607	arg1	incidence					1577:1585	The incidence	1573:1585	The incidence of EHIT class 2&ndash;4	1573:1609	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	2	theme	2&ndash	1601:1607	arg1	%					1618:1618	0.8%	1615:1618	0.8% (n = 6) in total	1615:1635	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	6	3	theme	therapeutic	1154:1164	arg1	anticoagulation					1166:1180	therapeutic anticoagulation	1154:1180	therapeutic anticoagulation	1154:1180	Of those, 35 patients were omitted because of therapeutic anticoagulation or dual antiplatelet therapy.					
31869428	10	4	theme	=	1695:1695	arg1	n					1693:1693	n = 1	1693:1697	n = 1	1693:1697	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	4	theme	=	1695:1695	arg1	%					1690:1690	0.3%	1687:1690	0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI]	1687:1779	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	11	5	from	%	1928:1928	arg1	0.0.12					1953:1958	0.0.12	1953:1958	0.0.12, CI 0.01&ndash;0.96, p = 0.046)	1953:1990	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	5	from	%	1928:1928	arg1	group					1913:1917	group 1	1913:1919	group 1	1913:1919	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	5	from	%	1928:1928	arg1	group					1941:1945	group 2	1941:1947	group 2	1941:1947	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	17	6	theme	more	2744:2747	arg1	conclusions					2760:2770	more definitive conclusions	2744:2770	more definitive conclusions	2744:2770	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	16	7	theme	safety	2593:2598	arg1	profile					2600:2606	safety profile	2593:2606	safety profile	2593:2606	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	12	8	theme	Propensity	1993:2002	arg1	analysis					2018:2025	Propensity score-matched analysis	1993:2025	Propensity score-matched analysis	1993:2025	Propensity score-matched analysis revealed no significant difference in the composite primary endpoint (CI &minus;0.074 to 0.26).					
31869428	16	9	with	treatment	2551:2559	arg1	rate					2584:2587	a similar success rate	2566:2587	a similar success rate	2566:2587	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	16	9	with	treatment	2551:2559	arg1	profile					2600:2606	safety profile	2593:2606	safety profile	2593:2606	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	10	10	dep	0.19	1750:1753	arg1	[CI					1776:1778	confidence interval [CI	1756:1778	confidence interval [CI	1756:1778	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	15	11	theme	score-matched	2299:2311	arg1	analysis					2313:2320	Propensity score-matched analysis	2288:2320	Propensity score-matched analysis	2288:2320	Propensity score-matched analysis showed no significant difference in the secondary endpoints.					
31869428	2	12	theme	veins	587:591	arg1	EVLA					557:560	outpatient EVLA	546:560	outpatient EVLA of truncal and accessory veins between February 2009 and December 2015	546:631	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	2	13	theme	retrospective	327:339	arg1	study					341:345	this multicentre retrospective study	310:345	this multicentre retrospective study in a university hospital, regional hospital and private practices	310:411	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	16	14	theme	treatment	2551:2559	arg1	days					2532:2535	3 days	2530:2535	3 days	2530:2535	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	16	14	theme	treatment	2551:2559	arg1	days					2543:2546	10 days	2540:2546	10 days	2540:2546	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	4	15	theme	secondary	915:923	arg1	endpoints					925:933	Cumulative secondary endpoints	904:933	Cumulative secondary endpoints	904:933	Cumulative secondary endpoints were complete ablation of the treated veins and major bleeding, skin burns and infection.					
31869428	14	16	theme	major	2240:2244	arg1	events					2255:2260	No PE or major bleeding events	2231:2260	No PE or major bleeding events	2231:2260	No PE or major bleeding events occurred in either group.					
31869428	3	17	theme	deep	747:750	arg1	thrombosis					757:766	deep vein thrombosis	747:766	deep vein thrombosis (DVT)	747:772	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	3	17	theme	deep	747:750	arg1	4					744:744	4	744:744	4	744:744	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	3	17	theme	deep	747:750	arg1	DVT					769:771	DVT	769:771	DVT	769:771	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	5	18	theme	patients	1048:1055	arg1	total					1035:1039	A total	1033:1039	RESULTS A total of 864 patients	1025:1055	RESULTS A total of 864 patients were treated with EVLA as an outpatient procedure.					
31869428	10	19	theme	=	1623:1623	arg1	n					1621:1621	n = 6	1621:1625	n = 6	1621:1625	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	19	theme	=	1623:1623	arg1	%					1618:1618	0.8%	1615:1618	0.8% (n = 6) in total	1615:1635	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	12	20	theme	composite	2069:2077	arg1	endpoint					2087:2094	the composite primary endpoint	2065:2094	the composite primary endpoint (CI &minus;0.074 to 0.26)	2065:2120	Propensity score-matched analysis revealed no significant difference in the composite primary endpoint (CI &minus;0.074 to 0.26).					
31869428	14	21	theme	PE	2234:2235	arg1	events					2255:2260	No PE or major bleeding events	2231:2260	No PE or major bleeding events	2231:2260	No PE or major bleeding events occurred in either group.					
31869428	11	22	theme	=	1905:1905	arg1	%					1900:1900	2.1%	1897:1900	2.1% (n = 8) in group 1	1897:1919	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	22	theme	=	1905:1905	arg1	n					1903:1903	n = 8	1903:1907	n = 8	1903:1907	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	3	23	theme	tomography	847:856	arg1	angiography					858:868	computed tomography angiography	838:868	computed tomography angiography	838:868	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	17	24	theme	pharmacological	2690:2704	arg1	thromboprophylaxis					2706:2723	pharmacological thromboprophylaxis	2690:2723	pharmacological thromboprophylaxis	2690:2723	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	1	25	theme	pharmacological	213:227	arg1	thromboprophylaxis					229:246	pharmacological thromboprophylaxis	213:246	pharmacological thromboprophylaxis	213:246	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	10	26	from	%	1618:1618	arg1	group					1654:1658	group 1	1654:1660	group 1 (treated for 3 days)	1654:1681	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	26	from	%	1618:1618	arg1	total					1631:1635	total	1631:1635	total	1631:1635	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	26	from	%	1618:1618	arg1	group					1703:1707	group 2	1703:1709	group 2 (treated for 10 days)	1703:1731	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	2	27	theme	accessory	577:585	arg1	veins					587:591	truncal and accessory veins	565:591	truncal and accessory veins between February 2009 and December 2015	565:631	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	15	28	theme	secondary	2362:2370	arg1	endpoints					2372:2380	the secondary endpoints	2358:2380	the secondary endpoints	2358:2380	Propensity score-matched analysis showed no significant difference in the secondary endpoints.					
31869428	6	29	theme	antiplatelet	1190:1201	arg1	therapy					1203:1209	dual antiplatelet therapy	1185:1209	dual antiplatelet therapy	1185:1209	Of those, 35 patients were omitted because of therapeutic anticoagulation or dual antiplatelet therapy.					
31869428	10	30	theme	=	1646:1646	arg1	n					1644:1644	n = 6	1644:1648	n = 6	1644:1648	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	30	theme	=	1646:1646	arg1	%					1641:1641	1.3%	1638:1641	1.3% (n = 6) in group 1 (treated for 3 days)	1638:1681	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	4	31	theme	veins	973:977	arg1	infection					1014:1022	infection	1014:1022	infection	1014:1022	Cumulative secondary endpoints were complete ablation of the treated veins and major bleeding, skin burns and infection.					
31869428	4	31	theme	veins	973:977	arg1	burns					1004:1008	skin burns	999:1008	skin burns	999:1008	Cumulative secondary endpoints were complete ablation of the treated veins and major bleeding, skin burns and infection.					
31869428	4	31	theme	veins	973:977	arg1	ablation					949:956	complete ablation	940:956	complete ablation of the treated veins	940:977	Cumulative secondary endpoints were complete ablation of the treated veins and major bleeding, skin burns and infection.					
31869428	13	32	from	%	2176:2176	arg1	group					2160:2164	group 1	2160:2166	group 1	2160:2166	Complete occlusion rate was 99.2% in group 1 and 98.8% in group 2 (OR 0.61, CI 0.15&ndash;2.59, p = 0.506).					
31869428	13	32	from	%	2176:2176	arg1	group					2181:2185	group 2	2181:2187	group 2	2181:2187	Complete occlusion rate was 99.2% in group 1 and 98.8% in group 2 (OR 0.61, CI 0.15&ndash;2.59, p = 0.506).					
31869428	11	33	theme	p	1981:1981	arg1	0.046					1985:1989	p = 0.046	1981:1989	p = 0.046	1981:1989	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	10	34	dep	%	1618:1618	arg1	1.66					1792:1795	1.66	1792:1795	1.66	1792:1795	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	34	dep	%	1618:1618	arg1	0.02&ndash					1781:1790	0.02&ndash	1781:1790	0.02&ndash	1781:1790	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	17	35	theme	pharmacological	2799:2813	arg1	thromboprophylaxis					2825:2842	pharmacological post-EVLA thromboprophylaxis	2799:2842	pharmacological post-EVLA thromboprophylaxis	2799:2842	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	17	36	theme	large	2624:2628	arg1	trial					2649:2653	a large randomised control trial	2622:2653	a large randomised control trial	2622:2653	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	11	37	theme	primary	1825:1831	arg1	endpoint					1843:1850	The cumulative primary composite endpoint	1810:1850	The cumulative primary composite endpoint at 4-week follow-up	1810:1870	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	37	theme	primary	1825:1831	arg1	%					1879:1879	1.1%	1876:1879	1.1% (n = 9)	1876:1887	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	2	38	with	patients	491:498	arg1	veins					514:518	varicose veins class C2 to C6	505:533	varicose veins class C2 to C6	505:533	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	2	39	theme	varicose	505:512	arg1	veins					514:518	varicose veins class C2 to C6	505:533	varicose veins class C2 to C6	505:533	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	13	40	dep	=	2221:2221	arg1	0.61					2193:2196	0.61	2193:2196	0.61	2193:2196	Complete occlusion rate was 99.2% in group 1 and 98.8% in group 2 (OR 0.61, CI 0.15&ndash;2.59, p = 0.506).					
31869428	13	40	dep	=	2221:2221	arg1	0.15&ndash					2202:2211	CI 0.15&ndash	2199:2211	CI 0.15&ndash	2199:2211	Complete occlusion rate was 99.2% in group 1 and 98.8% in group 2 (OR 0.61, CI 0.15&ndash;2.59, p = 0.506).					
31869428	13	40	dep	=	2221:2221	arg1	2.59					2213:2216	2.59	2213:2216	2.59	2213:2216	Complete occlusion rate was 99.2% in group 1 and 98.8% in group 2 (OR 0.61, CI 0.15&ndash;2.59, p = 0.506).					
31869428	17	41	theme	thromboprophylaxis	2825:2842	arg1	duration					2787:2794	the optimal duration	2775:2794	the optimal duration of pharmacological post-EVLA thromboprophylaxis	2775:2842	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	3	42	theme	duplex	817:822	arg1	ultrasound					824:833	duplex ultrasound	817:833	duplex ultrasound	817:833	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	1	43	theme	study	167:171	arg1	objective					149:157	The objective	145:157	The objective of this study	145:171	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	2	44	from	study	341:345	arg1	hospital					363:370	a university hospital	350:370	a university hospital	350:370	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	2	44	from	study	341:345	arg1	hospital					382:389	regional hospital	373:389	regional hospital	373:389	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	2	44	from	study	341:345	arg1	practices					403:411	private practices	395:411	private practices	395:411	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	3	45	theme	cumulative	638:647	arg1	endpoint					666:673	The cumulative primary efficacy endpoint	634:673	The cumulative primary efficacy endpoint	634:673	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	10	46	theme	ratio	1739:1743	arg1	0.19					1750:1753	odds ratio [OR] 0.19	1734:1753	odds ratio [OR] 0.19	1734:1753	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	46	theme	ratio	1739:1743	arg1	%					1690:1690	0.3%	1687:1690	0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI]	1687:1779	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	0	47	theme	score-matched	104:116	arg1	analysis					118:125	a propensity score-matched analysis	91:125	a propensity score-matched analysis	91:125	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation: a propensity score-matched analysis.					
31869428	9	48	theme	rivaroxaban	1474:1484	arg1	p.o.					1492:1495	rivaroxaban 10 mg p.o.	1474:1495	rivaroxaban 10 mg p.o. for 3 days	1474:1506	Of those, 225 patients (28.4%) received fondaparinux 2.5 mg s.c. for 3 days, 166 patients (20.9%) received rivaroxaban 10 mg p.o. for 3 days and 402 patients (50.7%) received rivaroxaban 10 mg for 10 days.					
31869428	3	49	theme	follow-up	893:901	arg1	weeks					884:888	1 and 4 weeks	876:888	1 and 4 weeks of follow-up	876:901	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	11	50	theme	=	1884:1884	arg1	n					1882:1882	n = 9	1882:1886	n = 9	1882:1886	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	50	theme	=	1884:1884	arg1	%					1879:1879	1.1%	1876:1879	1.1% (n = 9)	1876:1887	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	12	51	theme	score-matched	2004:2016	arg1	analysis					2018:2025	Propensity score-matched analysis	1993:2025	Propensity score-matched analysis	1993:2025	Propensity score-matched analysis revealed no significant difference in the composite primary endpoint (CI &minus;0.074 to 0.26).					
31869428	16	52	theme	pharmacological	2449:2463	arg1	thromboprophylaxis					2465:2482	pharmacological thromboprophylaxis	2449:2482	pharmacological thromboprophylaxis after EVLA	2449:2493	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	12	53	dep	endpoint	2087:2094	arg1	&minus					2100:2105	CI &minus	2097:2105	CI &minus;0.074 to 0.26	2097:2119	Propensity score-matched analysis revealed no significant difference in the composite primary endpoint (CI &minus;0.074 to 0.26).					
31869428	9	54	theme	mg	1489:1490	arg1	p.o.					1492:1495	rivaroxaban 10 mg p.o.	1474:1495	rivaroxaban 10 mg p.o. for 3 days	1474:1506	Of those, 225 patients (28.4%) received fondaparinux 2.5 mg s.c. for 3 days, 166 patients (20.9%) received rivaroxaban 10 mg p.o. for 3 days and 402 patients (50.7%) received rivaroxaban 10 mg for 10 days.					
31869428	10	55	from	%	1641:1641	arg1	group					1654:1658	group 1	1654:1660	group 1 (treated for 3 days)	1654:1681	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	55	from	%	1641:1641	arg1	total					1631:1635	total	1631:1635	total	1631:1635	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	55	from	%	1641:1641	arg1	group					1703:1707	group 2	1703:1709	group 2 (treated for 10 days)	1703:1731	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	11	56	theme	composite	1833:1841	arg1	endpoint					1843:1850	The cumulative primary composite endpoint	1810:1850	The cumulative primary composite endpoint at 4-week follow-up	1810:1870	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	56	theme	composite	1833:1841	arg1	%					1879:1879	1.1%	1876:1879	1.1% (n = 9)	1876:1887	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	2	57	theme	class	520:524	arg1	C2					526:527	class C2	520:527	varicose veins class C2 to C6	505:533	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	8	58	theme	final	1351:1355	arg1	analysis					1357:1364	the final analysis	1347:1364	the final analysis	1347:1364	A total of 793 were included in the final analysis.					
31869428	11	59	dep	0.01&ndash	1964:1973	arg1	0.96					1975:1978	0.96	1975:1978	0.96	1975:1978	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	59	dep	0.01&ndash	1964:1973	arg1	0.046					1985:1989	p = 0.046	1981:1989	p = 0.046	1981:1989	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	16	60	theme	score-matched	2411:2423	arg1	analysis					2425:2432	propensity score-matched analysis	2400:2432	propensity score-matched analysis	2400:2432	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	11	61	theme	cumulative	1814:1823	arg1	endpoint					1843:1850	The cumulative primary composite endpoint	1810:1850	The cumulative primary composite endpoint at 4-week follow-up	1810:1870	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	61	theme	cumulative	1814:1823	arg1	%					1879:1879	1.1%	1876:1879	1.1% (n = 9)	1876:1887	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	0	62	theme	pharmacological	12:26	arg1	thromboprophylaxis					28:45	pharmacological thromboprophylaxis	12:45	pharmacological thromboprophylaxis	12:45	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation: a propensity score-matched analysis.					
31869428	2	63	dep	C2	526:527	arg1	to					529:530	to	529:530	to	529:530	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	2	63	dep	C2	526:527	arg1	C6					532:533	C6	532:533	varicose veins class C2 to C6	505:533	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	2	64	theme	patients	491:498	arg1	data					467:470	the demographic, procedural and outcome data	427:470	the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015	427:631	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	3	65	theme	efficacy	657:664	arg1	endpoint					666:673	The cumulative primary efficacy endpoint	634:673	The cumulative primary efficacy endpoint	634:673	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	1	66	dep	was	173:175	arg1	AIM					128:130	AIM	128:130	AIM OF THE STUDY	128:143	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	10	67	theme	class	1595:1599	arg1	2&ndash					1601:1607	EHIT class 2&ndash;4	1590:1609	EHIT class 2&ndash;4	1590:1609	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	0	68	theme	endovenous	64:73	arg1	ablation					81:88	outpatient endovenous laser ablation	53:88	outpatient endovenous laser ablation	53:88	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation: a propensity score-matched analysis.					
31869428	13	69	from	%	2155:2155	arg1	group					2160:2164	group 1	2160:2166	group 1	2160:2166	Complete occlusion rate was 99.2% in group 1 and 98.8% in group 2 (OR 0.61, CI 0.15&ndash;2.59, p = 0.506).					
31869428	13	69	from	%	2155:2155	arg1	group					2181:2185	group 2	2181:2187	group 2	2181:2187	Complete occlusion rate was 99.2% in group 1 and 98.8% in group 2 (OR 0.61, CI 0.15&ndash;2.59, p = 0.506).					
31869428	7	70	theme	pharmacological	1268:1282	arg1	thromboprophylaxis					1284:1301	pharmacological thromboprophylaxis	1268:1301	pharmacological thromboprophylaxis	1268:1301	Another 36 cases were excluded as the patients received pharmacological thromboprophylaxis for 5 days.					
31869428	10	71	theme	interval	1767:1774	arg1	[CI					1776:1778	confidence interval [CI	1756:1778	confidence interval [CI	1756:1778	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	3	72	theme	thrombosis	712:721	arg1	2&ndash					736:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	12	73	dep	&minus	2100:2105	arg1	0.26					2116:2119	0.26	2116:2119	0.26	2116:2119	Propensity score-matched analysis revealed no significant difference in the composite primary endpoint (CI &minus;0.074 to 0.26).					
31869428	1	74	theme	thromboprophylaxis	229:246	arg1	duration					201:208	the optimal duration	189:208	the optimal duration of pharmacological thromboprophylaxis	189:246	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	2	75	dep	veins	514:518	arg1	C2					526:527	class C2	520:527	varicose veins class C2 to C6	505:533	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	3	76	theme	endovenous	688:697	arg1	2&ndash					736:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	1	77	theme	outpatient	254:263	arg1	ablation					282:289	outpatient endovenous laser ablation	254:289	outpatient endovenous laser ablation (EVLA)	254:296	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	1	77	theme	outpatient	254:263	arg1	EVLA					292:295	EVLA	292:295	EVLA	292:295	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	17	78	theme	randomised	2630:2639	arg1	trial					2649:2653	a large randomised control trial	2622:2653	a large randomised control trial	2622:2653	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	12	79	theme	significant	2039:2049	arg1	difference					2051:2060	no significant difference	2036:2060	no significant difference	2036:2060	Propensity score-matched analysis revealed no significant difference in the composite primary endpoint (CI &minus;0.074 to 0.26).					
31869428	3	80	theme	class	730:734	arg1	2&ndash					736:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	2	81	theme	regional	373:380	arg1	hospital					382:389	regional hospital	373:389	regional hospital	373:389	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	12	82	dep	0.26	2116:2119	arg1	to					2113:2114	to	2113:2114	to	2113:2114	Propensity score-matched analysis revealed no significant difference in the composite primary endpoint (CI &minus;0.074 to 0.26).					
31869428	1	83	theme	laser	276:280	arg1	ablation					282:289	outpatient endovenous laser ablation	254:289	outpatient endovenous laser ablation (EVLA)	254:296	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	1	83	theme	laser	276:280	arg1	EVLA					292:295	EVLA	292:295	EVLA	292:295	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	3	84	theme	EHIT	724:727	arg1	2&ndash					736:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	10	85	theme	=	1800:1800	arg1	p					1798:1798	p = 0.133	1798:1806	p = 0.133	1798:1806	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	11	86	theme	4-week	1855:1860	arg1	follow-up					1862:1870	4-week follow-up	1855:1870	4-week follow-up	1855:1870	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	17	87	theme	control	2641:2647	arg1	trial					2649:2653	a large randomised control trial	2622:2653	a large randomised control trial	2622:2653	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	5	88	dep	RESULTS	1025:1031	arg1	total					1035:1039	A total	1033:1039	RESULTS A total of 864 patients	1025:1055	RESULTS A total of 864 patients were treated with EVLA as an outpatient procedure.					
31869428	16	89	theme	propensity	2400:2409	arg1	analysis					2425:2432	propensity score-matched analysis	2400:2432	propensity score-matched analysis	2400:2432	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	9	90	theme	fondaparinux	1407:1418	arg1	s.c.					1427:1430	fondaparinux 2.5 mg s.c.	1407:1430	fondaparinux 2.5 mg s.c. for 3 days	1407:1441	Of those, 225 patients (28.4%) received fondaparinux 2.5 mg s.c. for 3 days, 166 patients (20.9%) received rivaroxaban 10 mg p.o. for 3 days and 402 patients (50.7%) received rivaroxaban 10 mg for 10 days.					
31869428	14	91	theme	bleeding	2246:2253	arg1	events					2255:2260	No PE or major bleeding events	2231:2260	No PE or major bleeding events	2231:2260	No PE or major bleeding events occurred in either group.					
31869428	2	92	theme	multicentre	315:325	arg1	study					341:345	this multicentre retrospective study	310:345	this multicentre retrospective study in a university hospital, regional hospital and private practices	310:411	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	1	93	theme	optimal	193:199	arg1	duration					201:208	the optimal duration	189:208	the optimal duration of pharmacological thromboprophylaxis	189:246	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	4	94	theme	Cumulative	904:913	arg1	endpoints					925:933	Cumulative secondary endpoints	904:933	Cumulative secondary endpoints	904:933	Cumulative secondary endpoints were complete ablation of the treated veins and major bleeding, skin burns and infection.					
31869428	15	95	theme	Propensity	2288:2297	arg1	analysis					2313:2320	Propensity score-matched analysis	2288:2320	Propensity score-matched analysis	2288:2320	Propensity score-matched analysis showed no significant difference in the secondary endpoints.					
31869428	16	96	dep	showed	2437:2442	arg1	Using					2394:2398	Using	2394:2398	Using propensity score-matched analysis	2394:2432	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	3	97	theme	vein	752:755	arg1	thrombosis					757:766	deep vein thrombosis	747:766	deep vein thrombosis (DVT)	747:772	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	3	97	theme	vein	752:755	arg1	4					744:744	4	744:744	4	744:744	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	3	97	theme	vein	752:755	arg1	DVT					769:771	DVT	769:771	DVT	769:771	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	10	98	from	%	1690:1690	arg1	group					1654:1658	group 1	1654:1660	group 1 (treated for 3 days)	1654:1681	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	98	from	%	1690:1690	arg1	total					1631:1635	total	1631:1635	total	1631:1635	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	98	from	%	1690:1690	arg1	group					1703:1707	group 2	1703:1709	group 2 (treated for 10 days)	1703:1731	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	2	99	theme	outpatient	546:555	arg1	EVLA					557:560	outpatient EVLA	546:560	outpatient EVLA of truncal and accessory veins between February 2009 and December 2015	546:631	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	3	100	theme	pulmonary	778:786	arg1	PE					798:799	PE	798:799	PE	798:799	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	3	100	theme	pulmonary	778:786	arg1	embolism					788:795	pulmonary embolism	778:795	pulmonary embolism (PE)	778:800	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	3	100	theme	pulmonary	778:786	arg1	4					744:744	4	744:744	4	744:744	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	2	101	theme	truncal	565:571	arg1	veins					587:591	truncal and accessory veins	565:591	truncal and accessory veins between February 2009 and December 2015	565:631	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	11	102	from	%	1879:1879	arg1	0.0.12					1953:1958	0.0.12	1953:1958	0.0.12, CI 0.01&ndash;0.96, p = 0.046)	1953:1990	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	102	from	%	1879:1879	arg1	group					1913:1917	group 1	1913:1919	group 1	1913:1919	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	102	from	%	1879:1879	arg1	group					1941:1945	group 2	1941:1947	group 2	1941:1947	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	17	103	theme	optimal	2779:2785	arg1	duration					2787:2794	the optimal duration	2775:2794	the optimal duration of pharmacological post-EVLA thromboprophylaxis	2775:2842	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	1	104	theme	STUDY	139:143	arg1	AIM					128:130	AIM	128:130	AIM OF THE STUDY	128:143	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	6	105	theme	dual	1185:1188	arg1	therapy					1203:1209	dual antiplatelet therapy	1185:1209	dual antiplatelet therapy	1185:1209	Of those, 35 patients were omitted because of therapeutic anticoagulation or dual antiplatelet therapy.					
31869428	2	106	theme	university	352:361	arg1	hospital					363:370	a university hospital	350:370	a university hospital	350:370	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	11	107	from	%	1900:1900	arg1	0.0.12					1953:1958	0.0.12	1953:1958	0.0.12, CI 0.01&ndash;0.96, p = 0.046)	1953:1990	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	107	from	%	1900:1900	arg1	group					1913:1917	group 1	1913:1919	group 1	1913:1919	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	107	from	%	1900:1900	arg1	group					1941:1945	group 2	1941:1947	group 2	1941:1947	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	9	108	theme	mg	1424:1425	arg1	s.c.					1427:1430	fondaparinux 2.5 mg s.c.	1407:1430	fondaparinux 2.5 mg s.c. for 3 days	1407:1441	Of those, 225 patients (28.4%) received fondaparinux 2.5 mg s.c. for 3 days, 166 patients (20.9%) received rivaroxaban 10 mg p.o. for 3 days and 402 patients (50.7%) received rivaroxaban 10 mg for 10 days.					
31869428	3	109	theme	computed	838:845	arg1	angiography					858:868	computed tomography angiography	838:868	computed tomography angiography	838:868	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	4	110	theme	treated	965:971	arg1	veins					973:977	the treated veins	961:977	the treated veins	961:977	Cumulative secondary endpoints were complete ablation of the treated veins and major bleeding, skin burns and infection.					
31869428	17	111	theme	post-EVLA	2815:2823	arg1	thromboprophylaxis					2825:2842	pharmacological post-EVLA thromboprophylaxis	2799:2842	pharmacological post-EVLA thromboprophylaxis	2799:2842	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	13	112	theme	Complete	2123:2130	arg1	rate					2142:2145	Complete occlusion rate	2123:2145	Complete occlusion rate	2123:2145	Complete occlusion rate was 99.2% in group 1 and 98.8% in group 2 (OR 0.61, CI 0.15&ndash;2.59, p = 0.506).					
31869428	0	113	theme	propensity	93:102	arg1	analysis					118:125	a propensity score-matched analysis	91:125	a propensity score-matched analysis	91:125	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation: a propensity score-matched analysis.					
31869428	11	114	theme	=	1983:1983	arg1	0.046					1985:1989	p = 0.046	1981:1989	p = 0.046	1981:1989	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	10	115	theme	[OR	1745:1747	arg1	0.19					1750:1753	odds ratio [OR] 0.19	1734:1753	odds ratio [OR] 0.19	1734:1753	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	115	theme	[OR	1745:1747	arg1	%					1690:1690	0.3%	1687:1690	0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI]	1687:1779	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	15	116	from	difference	2344:2353	arg1	endpoints					2372:2380	the secondary endpoints	2358:2380	the secondary endpoints	2358:2380	Propensity score-matched analysis showed no significant difference in the secondary endpoints.					
31869428	4	117	theme	complete	940:947	arg1	ablation					949:956	complete ablation	940:956	complete ablation of the treated veins	940:977	Cumulative secondary endpoints were complete ablation of the treated veins and major bleeding, skin burns and infection.					
31869428	11	118	theme	=	1933:1933	arg1	%					1928:1928	0.3%	1925:1928	0.3% (n = 1)	1925:1936	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	11	118	theme	=	1933:1933	arg1	n					1931:1931	n = 1	1931:1935	n = 1	1931:1935	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	8	119	theme	793	1326:1328	arg1	total					1317:1321	A total	1315:1321	A total of 793	1315:1328	A total of 793 were included in the final analysis.					
31869428	11	120	dep	0.0.12	1953:1958	arg1	0.01&ndash					1964:1973	CI 0.01&ndash	1961:1973	CI 0.01&ndash	1961:1973	The cumulative primary composite endpoint at 4-week follow-up was 1.1% (n = 9) and was 2.1% (n = 8) in group 1 and 0.3% (n = 1) in group 2 (OR 0.0.12, CI 0.01&ndash;0.96, p = 0.046).					
31869428	10	121	theme	odds	1734:1737	arg1	0.19					1750:1753	odds ratio [OR] 0.19	1734:1753	odds ratio [OR] 0.19	1734:1753	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	10	121	theme	odds	1734:1737	arg1	%					1690:1690	0.3%	1687:1690	0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI]	1687:1779	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	13	122	theme	occlusion	2132:2140	arg1	rate					2142:2145	Complete occlusion rate	2123:2145	Complete occlusion rate	2123:2145	Complete occlusion rate was 99.2% in group 1 and 98.8% in group 2 (OR 0.61, CI 0.15&ndash;2.59, p = 0.506).					
31869428	9	123	theme	10	1486:1487	arg1	mg					1489:1490	mg	1489:1490	mg	1489:1490	Of those, 225 patients (28.4%) received fondaparinux 2.5 mg s.c. for 3 days, 166 patients (20.9%) received rivaroxaban 10 mg p.o. for 3 days and 402 patients (50.7%) received rivaroxaban 10 mg for 10 days.					
31869428	15	124	theme	significant	2332:2342	arg1	difference					2344:2353	no significant difference	2329:2353	no significant difference in the secondary endpoints	2329:2380	Propensity score-matched analysis showed no significant difference in the secondary endpoints.					
31869428	5	125	theme	outpatient	1086:1095	arg1	procedure					1097:1105	an outpatient procedure	1083:1105	an outpatient procedure	1083:1105	RESULTS A total of 864 patients were treated with EVLA as an outpatient procedure.					
31869428	5	125	theme	outpatient	1086:1095	arg1	RESULTS					1025:1031	RESULTS	1025:1031	RESULTS A total of 864 patients	1025:1055	RESULTS A total of 864 patients were treated with EVLA as an outpatient procedure.					
31869428	2	126	theme	outcome	459:465	arg1	data					467:470	the demographic, procedural and outcome data	427:470	the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015	427:631	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	16	127	with	effective	2515:2523	arg1	days					2532:2535	3 days	2530:2535	3 days	2530:2535	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	16	127	with	effective	2515:2523	arg1	days					2543:2546	10 days	2540:2546	10 days	2540:2546	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	17	128	theme	definitive	2749:2758	arg1	conclusions					2760:2770	more definitive conclusions	2744:2770	more definitive conclusions	2744:2770	Undoubtedly, a large randomised control trial, ideally including a group without pharmacological thromboprophylaxis, is needed to draw more definitive conclusions on the optimal duration of pharmacological post-EVLA thromboprophylaxis.					
31869428	2	129	theme	private	395:401	arg1	practices					403:411	private practices	395:411	private practices	395:411	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	0	130	theme	thromboprophylaxis	28:45	arg1	Duration					0:7	Duration	0:7	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation	0:88	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation: a propensity score-matched analysis.					
31869428	16	131	theme	similar	2568:2574	arg1	rate					2584:2587	a similar success rate	2566:2587	a similar success rate	2566:2587	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	2	132	theme	procedural	444:453	arg1	data					467:470	the demographic, procedural and outcome data	427:470	the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015	427:631	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	0	133	dep	analysis	118:125	arg1	Duration					0:7	Duration	0:7	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation	0:88	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation: a propensity score-matched analysis.					
31869428	2	134	theme	demographic	431:441	arg1	data					467:470	the demographic, procedural and outcome data	427:470	the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015	427:631	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	0	135	theme	outpatient	53:62	arg1	ablation					81:88	outpatient endovenous laser ablation	53:88	outpatient endovenous laser ablation	53:88	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation: a propensity score-matched analysis.					
31869428	2	136	theme	consecutive	479:489	arg1	patients					491:498	all consecutive patients	475:498	all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015	475:631	METHODS In this multicentre retrospective study in a university hospital, regional hospital and private practices, we collected the demographic, procedural and outcome data of all consecutive patients with varicose veins class C2 to C6 undergoing outpatient EVLA of truncal and accessory veins between February 2009 and December 2015.					
31869428	3	137	theme	primary	649:655	arg1	endpoint					666:673	The cumulative primary efficacy endpoint	634:673	The cumulative primary efficacy endpoint	634:673	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	0	138	theme	laser	75:79	arg1	ablation					81:88	outpatient endovenous laser ablation	53:88	outpatient endovenous laser ablation	53:88	Duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation: a propensity score-matched analysis.					
31869428	4	139	theme	skin	999:1002	arg1	burns					1004:1008	skin burns	999:1008	skin burns	999:1008	Cumulative secondary endpoints were complete ablation of the treated veins and major bleeding, skin burns and infection.					
31869428	3	140	theme	heat-induced	699:710	arg1	2&ndash					736:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	endovenous heat-induced thrombosis (EHIT) class 2&ndash	688:742	The cumulative primary efficacy endpoint consisted of endovenous heat-induced thrombosis (EHIT) class 2&ndash;4, deep vein thrombosis (DVT) and pulmonary embolism (PE) diagnosed with duplex ultrasound or computed tomography angiography after 1 and 4 weeks of follow-up.					
31869428	10	141	theme	EHIT	1590:1593	arg1	2&ndash					1601:1607	EHIT class 2&ndash;4	1590:1609	EHIT class 2&ndash;4	1590:1609	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	16	142	theme	success	2576:2582	arg1	rate					2584:2587	a similar success rate	2566:2587	a similar success rate	2566:2587	CONCLUSION Using propensity score-matched analysis we showed that pharmacological thromboprophylaxis after EVLA seems to be equally effective with 3 days or 10 days of treatment with a similar success rate and safety profile.					
31869428	10	143	dep	1.66	1792:1795	arg1	p					1798:1798	p = 0.133	1798:1806	p = 0.133	1798:1806	The incidence of EHIT class 2&ndash;4 was 0.8% (n = 6) in total, 1.3% (n = 6) in group 1 (treated for 3 days) and 0.3% (n = 1) in group 2 (treated for 10 days) (odds ratio [OR] 0.19, confidence interval [CI] 0.02&ndash;1.66, p = 0.133).					
31869428	12	144	theme	primary	2079:2085	arg1	endpoint					2087:2094	the composite primary endpoint	2065:2094	the composite primary endpoint (CI &minus;0.074 to 0.26)	2065:2120	Propensity score-matched analysis revealed no significant difference in the composite primary endpoint (CI &minus;0.074 to 0.26).					
31869428	1	145	theme	endovenous	265:274	arg1	ablation					282:289	outpatient endovenous laser ablation	254:289	outpatient endovenous laser ablation (EVLA)	254:296	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
31869428	1	145	theme	endovenous	265:274	arg1	EVLA					292:295	EVLA	292:295	EVLA	292:295	AIM OF THE STUDY The objective of this study was to identify the optimal duration of pharmacological thromboprophylaxis after outpatient endovenous laser ablation (EVLA).					
30051965	0	0	theme	High-Fat	73:80	arg1	Diet					100:103	High-Fat, High-Cholesterol Diet	73:103	High-Fat, High-Cholesterol Diet	73:103	Galactomannan More than Pectin Exacerbates Liver Injury in Mice Fed with High-Fat, High-Cholesterol Diet.					
30051965	7	1	theme	weight	869:874	arg1	gain					876:879	lesser weight gain	862:879	lesser weight gain	862:879	Galactomannan leads to lesser weight gain and improved glucose tolerance, but increased liver damage.					
30051965	8	2	theme	enzymes	983:989	arg1	levels					967:972	elevated levels	958:972	elevated levels of liver enzymes	958:989	This is shown by elevated levels of liver enzymes compared to that with HCD alone.					
30051965	10	3	theme	disturbed	1257:1265	arg1	metabolism					1267:1276	disturbed metabolism	1257:1276	disturbed metabolism	1257:1276	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	2	4	theme	steatohepatitis	331:345	arg1	model					354:358	a nonalcoholic steatohepatitis (NASH) model	316:358	a nonalcoholic steatohepatitis (NASH) model	316:358	The study aims to investigate the fiber's effect on a nonalcoholic steatohepatitis (NASH) model.					
30051965	1	5	theme	lipid	246:250	arg1	metabolism					252:261	lipid metabolism	246:261	lipid metabolism	246:261	SCOPE Galactomannan and citrus pectin are considered 'super fibers' known for altering gut microbiota composition and improving glucose and lipid metabolism.					
30051965	3	6	dep	METHODS	361:367	arg1	experiments					393:403	Two feeding experiments	381:403	METHODS AND RESULTS Two feeding experiments	361:403	METHODS AND RESULTS Two feeding experiments are carried out using groups of 7-8 week-old male C57BL/6J mice.					
30051965	2	7	from	effect	306:311	arg1	model					354:358	a nonalcoholic steatohepatitis (NASH) model	316:358	a nonalcoholic steatohepatitis (NASH) model	316:358	The study aims to investigate the fiber's effect on a nonalcoholic steatohepatitis (NASH) model.					
30051965	9	8	theme	higher	1038:1043	arg1	steatosis					1045:1053	higher steatosis	1038:1053	higher steatosis	1038:1053	Fibers induce higher steatosis, as evaluated by liver histology.					
30051965	12	9	theme	microbiota	1413:1422	arg1	composition					1424:1434	the microbiota composition	1409:1434	the microbiota composition	1409:1434	Changes in the microbiota composition may answer this enigmatic observation.					
30051965	6	10	theme	Liver	769:773	arg1	damage					775:780	Liver damage	769:780	Liver damage	769:780	Liver damage is evaluated by histological and biochemical parameters.					
30051965	1	11	theme	citrus	130:135	arg1	pectin					137:142	citrus pectin	130:142	citrus pectin	130:142	SCOPE Galactomannan and citrus pectin are considered 'super fibers' known for altering gut microbiota composition and improving glucose and lipid metabolism.					
30051965	3	12	theme	mice	464:467	arg1	mice					464:467	7-8 week-old male C57BL/6J mice	437:467	7-8 week-old male C57BL/6J mice	437:467	METHODS AND RESULTS Two feeding experiments are carried out using groups of 7-8 week-old male C57BL/6J mice.					
30051965	3	12	theme	mice	464:467	arg1	groups					427:432	groups	427:432	groups of 7-8 week-old male C57BL/6J mice	427:467	METHODS AND RESULTS Two feeding experiments are carried out using groups of 7-8 week-old male C57BL/6J mice.					
30051965	10	13	theme	various	1125:1131	arg1	levels					1196:1201	elevated Proteobacteria levels	1172:1201	elevated Proteobacteria levels in the galactomannan group	1172:1228	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	10	13	theme	various	1125:1131	arg1	changes					1133:1139	various changes	1125:1139	various changes	1125:1139	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	10	14	theme	gut	1148:1150	arg1	microbiota					1152:1161	the gut microbiota	1144:1161	the gut microbiota	1144:1161	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	1	15	theme	super	160:164	arg1	fibers					166:171	super fibers	160:171	'super fibers' known for altering gut microbiota composition and improving glucose and lipid metabolism	159:261	SCOPE Galactomannan and citrus pectin are considered 'super fibers' known for altering gut microbiota composition and improving glucose and lipid metabolism.					
30051965	3	16	theme	feeding	385:391	arg1	experiments					393:403	Two feeding experiments	381:403	METHODS AND RESULTS Two feeding experiments	361:403	METHODS AND RESULTS Two feeding experiments are carried out using groups of 7-8 week-old male C57BL/6J mice.					
30051965	10	17	theme	galactomannan	1210:1222	arg1	group					1224:1228	the galactomannan group	1206:1228	the galactomannan group	1206:1228	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	4	18	theme	high	500:503	arg1	diet					525:528	a high cholesterol/cholate diet	498:528	a high cholesterol/cholate diet (HCD)	498:534	The diets used are based on a high cholesterol/cholate diet (HCD), such as a nutritional NASH model.					
30051965	4	18	theme	high	500:503	arg1	HCD					531:533	HCD	531:533	HCD	531:533	The diets used are based on a high cholesterol/cholate diet (HCD), such as a nutritional NASH model.					
30051965	9	19	theme	liver	1072:1076	arg1	histology					1078:1086	liver histology	1072:1086	liver histology	1072:1086	Fibers induce higher steatosis, as evaluated by liver histology.					
30051965	0	20	dep	High-Fat	73:80	arg1	High-Cholesterol					83:98	High-Cholesterol	83:98	High-Cholesterol	83:98	Galactomannan More than Pectin Exacerbates Liver Injury in Mice Fed with High-Fat, High-Cholesterol Diet.					
30051965	0	21	from	Injury	49:54	arg1	Mice					59:62	Mice	59:62	Mice Fed with High-Fat, High-Cholesterol Diet	59:103	Galactomannan More than Pectin Exacerbates Liver Injury in Mice Fed with High-Fat, High-Cholesterol Diet.					
30051965	8	22	theme	liver	977:981	arg1	enzymes					983:989	liver enzymes	977:989	liver enzymes	977:989	This is shown by elevated levels of liver enzymes compared to that with HCD alone.					
30051965	12	23	from	Changes	1398:1404	arg1	composition					1424:1434	the microbiota composition	1409:1434	the microbiota composition	1409:1434	Changes in the microbiota composition may answer this enigmatic observation.					
30051965	10	24	theme	elevated	1172:1179	arg1	levels					1196:1201	elevated Proteobacteria levels	1172:1201	elevated Proteobacteria levels in the galactomannan group	1172:1228	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	5	25	theme	second	749:754	arg1	HCD					744:746	the HCD	740:746	the HCD (second experiment)	740:766	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	5	25	theme	second	749:754	arg1	experiment					756:765	second experiment	749:765	second experiment	749:765	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	0	26	theme	Pectin	24:29	arg1	Injury					49:54	Pectin Exacerbates Liver Injury	24:54	Pectin Exacerbates Liver Injury in Mice Fed with High-Fat, High-Cholesterol Diet	24:103	Galactomannan More than Pectin Exacerbates Liver Injury in Mice Fed with High-Fat, High-Cholesterol Diet.					
30051965	4	27	theme	cholesterol/cholate	505:523	arg1	diet					525:528	a high cholesterol/cholate diet	498:528	a high cholesterol/cholate diet (HCD)	498:534	The diets used are based on a high cholesterol/cholate diet (HCD), such as a nutritional NASH model.					
30051965	4	27	theme	cholesterol/cholate	505:523	arg1	HCD					531:533	HCD	531:533	HCD	531:533	The diets used are based on a high cholesterol/cholate diet (HCD), such as a nutritional NASH model.					
30051965	8	28	theme	elevated	958:965	arg1	levels					967:972	elevated levels	958:972	elevated levels of liver enzymes	958:989	This is shown by elevated levels of liver enzymes compared to that with HCD alone.					
30051965	10	29	from	changes	1133:1139	arg1	microbiota					1152:1161	the gut microbiota	1144:1161	the gut microbiota	1144:1161	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	10	30	theme	Proteobacteria	1181:1194	arg1	levels					1196:1201	elevated Proteobacteria levels	1172:1201	elevated Proteobacteria levels in the galactomannan group	1172:1228	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	7	31	theme	increased	917:925	arg1	damage					933:938	increased liver damage	917:938	increased liver damage	917:938	Galactomannan leads to lesser weight gain and improved glucose tolerance, but increased liver damage.					
30051965	11	32	theme	mouse	1315:1319	arg1	model					1321:1325	a NASH mouse model	1308:1325	a NASH mouse model	1308:1325	CONCLUSIONS In a NASH mouse model, galactomannan increases liver damage but improves glucose metabolism.					
30051965	3	33	theme	week-old	441:448	arg1	mice					464:467	7-8 week-old male C57BL/6J mice	437:467	7-8 week-old male C57BL/6J mice	437:467	METHODS AND RESULTS Two feeding experiments are carried out using groups of 7-8 week-old male C57BL/6J mice.					
30051965	0	34	theme	Liver	43:47	arg1	Injury					49:54	Pectin Exacerbates Liver Injury	24:54	Pectin Exacerbates Liver Injury in Mice Fed with High-Fat, High-Cholesterol Diet	24:103	Galactomannan More than Pectin Exacerbates Liver Injury in Mice Fed with High-Fat, High-Cholesterol Diet.					
30051965	11	35	theme	NASH	1310:1313	arg1	model					1321:1325	a NASH mouse model	1308:1325	a NASH mouse model	1308:1325	CONCLUSIONS In a NASH mouse model, galactomannan increases liver damage but improves glucose metabolism.					
30051965	5	36	theme	prior	731:735	arg1	weeks					725:729	3 weeks	723:729	3 weeks prior to the HCD (second experiment)	723:766	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	1	37	theme	gut	193:195	arg1	composition					208:218	gut microbiota composition	193:218	gut microbiota composition	193:218	SCOPE Galactomannan and citrus pectin are considered 'super fibers' known for altering gut microbiota composition and improving glucose and lipid metabolism.					
30051965	0	38	theme	Exacerbates	31:41	arg1	Injury					49:54	Pectin Exacerbates Liver Injury	24:54	Pectin Exacerbates Liver Injury in Mice Fed with High-Fat, High-Cholesterol Diet	24:103	Galactomannan More than Pectin Exacerbates Liver Injury in Mice Fed with High-Fat, High-Cholesterol Diet.					
30051965	10	39	attach	linked	1115:1120	arg1	changes					1133:1139	various changes	1125:1139	various changes	1125:1139	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	10	39	attach	linked	1115:1120	arg1	levels					1196:1201	elevated Proteobacteria levels	1172:1201	elevated Proteobacteria levels in the galactomannan group	1172:1228	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	10	39	attach	linked	1115:1120	arg2	result					1105:1110	This intriguing result	1089:1110	This intriguing result	1089:1110	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	11	40	dep	CONCLUSIONS	1293:1303	arg1	model					1321:1325	a NASH mouse model	1308:1325	a NASH mouse model	1308:1325	CONCLUSIONS In a NASH mouse model, galactomannan increases liver damage but improves glucose metabolism.					
30051965	1	41	theme	microbiota	197:206	arg1	composition					208:218	gut microbiota composition	193:218	gut microbiota composition	193:218	SCOPE Galactomannan and citrus pectin are considered 'super fibers' known for altering gut microbiota composition and improving glucose and lipid metabolism.					
30051965	5	42	theme	first	688:692	arg1	HCD					683:685	the HCD	679:685	the HCD (first experiment)	679:704	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	5	42	theme	first	688:692	arg1	experiment					694:703	first experiment	688:703	first experiment	688:703	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	6	43	theme	biochemical	815:825	arg1	parameters					827:836	histological and biochemical parameters	798:836	histological and biochemical parameters	798:836	Liver damage is evaluated by histological and biochemical parameters.					
30051965	7	44	theme	liver	927:931	arg1	damage					933:938	increased liver damage	917:938	increased liver damage	917:938	Galactomannan leads to lesser weight gain and improved glucose tolerance, but increased liver damage.					
30051965	2	45	theme	nonalcoholic	318:329	arg1	NASH					348:351	NASH	348:351	NASH	348:351	The study aims to investigate the fiber's effect on a nonalcoholic steatohepatitis (NASH) model.					
30051965	2	45	theme	nonalcoholic	318:329	arg1	steatohepatitis					331:345	nonalcoholic steatohepatitis	318:345	a nonalcoholic steatohepatitis (NASH) model	316:358	The study aims to investigate the fiber's effect on a nonalcoholic steatohepatitis (NASH) model.					
30051965	1	46	theme	SCOPE	106:110	arg1	Galactomannan					112:124	SCOPE Galactomannan	106:124	SCOPE Galactomannan	106:124	SCOPE Galactomannan and citrus pectin are considered 'super fibers' known for altering gut microbiota composition and improving glucose and lipid metabolism.					
30051965	5	47	theme	15	607:608	arg1	%					609:609	%	609:609	%	609:609	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	7	48	theme	improved	885:892	arg1	tolerance					902:910	improved glucose tolerance	885:910	improved glucose tolerance	885:910	Galactomannan leads to lesser weight gain and improved glucose tolerance, but increased liver damage.					
30051965	6	49	theme	histological	798:809	arg1	parameters					827:836	histological and biochemical parameters	798:836	histological and biochemical parameters	798:836	Liver damage is evaluated by histological and biochemical parameters.					
30051965	5	50	theme	%	609:609	arg1	HCD-CP					632:637	HCD-CP	632:637	HCD-CP	632:637	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	5	50	theme	%	609:609	arg1	pectin					624:629	15% fiber-citrus pectin	607:629	15% fiber-citrus pectin (HCD-CP)	607:638	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	7	51	theme	glucose	894:900	arg1	tolerance					902:910	improved glucose tolerance	885:910	improved glucose tolerance	885:910	Galactomannan leads to lesser weight gain and improved glucose tolerance, but increased liver damage.					
30051965	3	52	theme	male	450:453	arg1	mice					464:467	7-8 week-old male C57BL/6J mice	437:467	7-8 week-old male C57BL/6J mice	437:467	METHODS AND RESULTS Two feeding experiments are carried out using groups of 7-8 week-old male C57BL/6J mice.					
30051965	4	53	theme	nutritional	547:557	arg1	model					564:568	a nutritional NASH model	545:568	a nutritional NASH model	545:568	The diets used are based on a high cholesterol/cholate diet (HCD), such as a nutritional NASH model.					
30051965	5	54	theme	fiber-citrus	611:622	arg1	HCD-CP					632:637	HCD-CP	632:637	HCD-CP	632:637	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	5	54	theme	fiber-citrus	611:622	arg1	pectin					624:629	15% fiber-citrus pectin	607:629	15% fiber-citrus pectin (HCD-CP)	607:638	Mice are fed a diet with or without 15% fiber-citrus pectin (HCD-CP) or galactomannan (HCD-G) together with the HCD (first experiment), which commenced 3 weeks prior to the HCD (second experiment).					
30051965	12	55	theme	enigmatic	1452:1460	arg1	observation					1462:1472	this enigmatic observation	1447:1472	this enigmatic observation	1447:1472	Changes in the microbiota composition may answer this enigmatic observation.					
30051965	3	56	theme	C57BL/6J	455:462	arg1	mice					464:467	7-8 week-old male C57BL/6J mice	437:467	7-8 week-old male C57BL/6J mice	437:467	METHODS AND RESULTS Two feeding experiments are carried out using groups of 7-8 week-old male C57BL/6J mice.					
30051965	10	57	theme	intriguing	1094:1103	arg1	result					1105:1110	This intriguing result	1089:1110	This intriguing result	1089:1110	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	10	58	from	levels	1196:1201	arg1	group					1224:1228	the galactomannan group	1206:1228	the galactomannan group	1206:1228	This intriguing result is linked to various changes in the gut microbiota, such as elevated Proteobacteria levels in the galactomannan group, which are correlated with disturbed metabolism and dysbiosis.					
30051965	11	59	theme	glucose	1378:1384	arg1	metabolism					1386:1395	glucose metabolism	1378:1395	glucose metabolism	1378:1395	CONCLUSIONS In a NASH mouse model, galactomannan increases liver damage but improves glucose metabolism.					
30051965	11	60	theme	liver	1352:1356	arg1	damage					1358:1363	liver damage	1352:1363	liver damage	1352:1363	CONCLUSIONS In a NASH mouse model, galactomannan increases liver damage but improves glucose metabolism.					
30051965	7	61	theme	lesser	862:867	arg1	gain					876:879	lesser weight gain	862:879	lesser weight gain	862:879	Galactomannan leads to lesser weight gain and improved glucose tolerance, but increased liver damage.					
30051965	4	62	theme	NASH	559:562	arg1	model					564:568	a nutritional NASH model	545:568	a nutritional NASH model	545:568	The diets used are based on a high cholesterol/cholate diet (HCD), such as a nutritional NASH model.					
31221339	3	0	from	stable	441:446	arg1	fluid					469:473	simulated gastric fluid	451:473	simulated gastric fluid with or without pepsin and mucus	451:506	We first demonstrated that taxifolin is stable in simulated gastric fluid with or without pepsin and mucus, and is able to cross pig gastric mucus layer and stomach mucosa.					
31221339	1	1	dep	possesses	94:102	arg1	instabile					179:187	instabile	179:187	instabile	179:187	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	6	2	theme	optimized	821:829	arg1	able					851:854	able	851:854	able	851:854	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	6	2	theme	optimized	821:829	arg1	microparticles					831:844	The optimized microparticles	817:844	The optimized microparticles	817:844	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	6	2	theme	optimized	821:829	arg1	adhere					884:889	adhere	884:889	to adhere to pig stomach mucosa for 5 h	881:919	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	6	2	theme	optimized	821:829	arg1	release					859:865	release	859:865	to release taxifolin	856:875	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	2	3	theme	taxifolin	348:356	arg1	time					368:371	taxifolin residence time	348:371	taxifolin residence time	348:371	The purpose of the work was therefore to produce a gastroadhesive formulation to prolong taxifolin residence time and release in the stomach.					
31221339	6	4	theme	stomach	898:904	arg1	mucosa					906:911	pig stomach mucosa	894:911	pig stomach mucosa	894:911	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	3	5	theme	cross	524:528	arg1	pig					530:532	cross pig gastric mucus layer and stomach mucosa	524:571	pig	530:532	We first demonstrated that taxifolin is stable in simulated gastric fluid with or without pepsin and mucus, and is able to cross pig gastric mucus layer and stomach mucosa.					
31221339	2	6	theme	work	278:281	arg1	purpose					263:269	The purpose	259:269	The purpose of the work	259:281	The purpose of the work was therefore to produce a gastroadhesive formulation to prolong taxifolin residence time and release in the stomach.					
31221339	1	7	theme	low	154:156	arg1	solubility					164:173	low water solubility	154:173	low water solubility	154:173	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	6	8	theme	pig	894:896	arg1	mucosa					906:911	pig stomach mucosa	894:911	pig stomach mucosa	894:911	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	5	9	theme	mean	771:774	arg1	diameter					794:801	a mean volume-equivalent diameter	769:801	a mean volume-equivalent diameter around 12 μm	769:814	Microparticles were characterized by a spherical shape and a mean volume-equivalent diameter around 12 μm.					
31221339	4	10	theme	mesoporous	639:648	arg1	silica					650:655	Syloid® AL-1 mesoporous silica	626:655	Syloid® AL-1 mesoporous silica	626:655	Next, gastromucoadhesive microparticles composed of Syloid® AL-1 mesoporous silica, chitosan and HPMC were produced using spray-drying.					
31221339	0	11	theme	composite	9:17	arg1	microparticles					19:32	Chitosan composite microparticles	0:32	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.	0:82	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.					
31221339	3	12	theme	mucus	542:546	arg1	layer					548:552	gastric mucus layer	534:552	gastric mucus layer	534:552	We first demonstrated that taxifolin is stable in simulated gastric fluid with or without pepsin and mucus, and is able to cross pig gastric mucus layer and stomach mucosa.					
31221339	1	13	theme	alkaline	192:199	arg1	medium					201:206	alkaline medium	192:206	alkaline medium	192:206	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	0	14	theme	Chitosan	0:7	arg1	microparticles					19:32	Chitosan composite microparticles	0:32	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.	0:82	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.					
31221339	1	15	theme	gastroprotective	104:119	arg1	property					121:128	gastroprotective property	104:128	gastroprotective property	104:128	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	3	16	theme	gastric	461:467	arg1	fluid					469:473	simulated gastric fluid	451:473	simulated gastric fluid with or without pepsin and mucus	451:506	We first demonstrated that taxifolin is stable in simulated gastric fluid with or without pepsin and mucus, and is able to cross pig gastric mucus layer and stomach mucosa.					
31221339	3	17	theme	gastric	534:540	arg1	layer					548:552	gastric mucus layer	534:552	gastric mucus layer	534:552	We first demonstrated that taxifolin is stable in simulated gastric fluid with or without pepsin and mucus, and is able to cross pig gastric mucus layer and stomach mucosa.					
31221339	3	18	dep	pig	530:532	arg1	layer					548:552	gastric mucus layer	534:552	gastric mucus layer	534:552	We first demonstrated that taxifolin is stable in simulated gastric fluid with or without pepsin and mucus, and is able to cross pig gastric mucus layer and stomach mucosa.					
31221339	0	19	theme	promising	37:45	arg1	system					62:67	A promising gastroadhesive system	35:67	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.	0:82	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.					
31221339	6	20	dep	able	851:854	arg1	able					851:854	able	851:854	able	851:854	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	6	20	dep	able	851:854	arg1	adhere					884:889	adhere	884:889	to adhere to pig stomach mucosa for 5 h	881:919	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	6	20	dep	able	851:854	arg1	microparticles					831:844	The optimized microparticles	817:844	The optimized microparticles	817:844	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	6	20	dep	able	851:854	arg1	release					859:865	release	859:865	to release taxifolin	856:875	The optimized microparticles were able to release taxifolin and to adhere to pig stomach mucosa for 5 h.					
31221339	4	21	theme	AL-1	634:637	arg1	silica					650:655	Syloid® AL-1 mesoporous silica	626:655	Syloid® AL-1 mesoporous silica	626:655	Next, gastromucoadhesive microparticles composed of Syloid® AL-1 mesoporous silica, chitosan and HPMC were produced using spray-drying.					
31221339	5	22	theme	volume-equivalent	776:792	arg1	diameter					794:801	a mean volume-equivalent diameter	769:801	a mean volume-equivalent diameter around 12 μm	769:814	Microparticles were characterized by a spherical shape and a mean volume-equivalent diameter around 12 μm.					
31221339	2	23	theme	residence	358:366	arg1	time					368:371	taxifolin residence time	348:371	taxifolin residence time	348:371	The purpose of the work was therefore to produce a gastroadhesive formulation to prolong taxifolin residence time and release in the stomach.					
31221339	3	24	theme	simulated	451:459	arg1	fluid					469:473	simulated gastric fluid	451:473	simulated gastric fluid with or without pepsin and mucus	451:506	We first demonstrated that taxifolin is stable in simulated gastric fluid with or without pepsin and mucus, and is able to cross pig gastric mucus layer and stomach mucosa.					
31221339	0	25	theme	gastroadhesive	47:60	arg1	system					62:67	A promising gastroadhesive system	35:67	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.	0:82	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.					
31221339	3	26	theme	stomach	558:564	arg1	mucosa					566:571	stomach mucosa	558:571	stomach mucosa	558:571	We first demonstrated that taxifolin is stable in simulated gastric fluid with or without pepsin and mucus, and is able to cross pig gastric mucus layer and stomach mucosa.					
31221339	1	27	contain	possesses	94:102	arg1	Taxifolin					84:92	Taxifolin	84:92	Taxifolin	84:92	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	1	27	contain	possesses	94:102	arg2	property					121:128	gastroprotective property	104:128	gastroprotective property	104:128	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	3	28	from	fluid	469:473	arg1	stable					441:446	stable	441:446	stable	441:446	We first demonstrated that taxifolin is stable in simulated gastric fluid with or without pepsin and mucus, and is able to cross pig gastric mucus layer and stomach mucosa.					
31221339	0	29	dep	microparticles	19:32	arg1	system					62:67	A promising gastroadhesive system	35:67	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.	0:82	Chitosan composite microparticles: A promising gastroadhesive system for taxifolin.					
31221339	2	30	theme	gastroadhesive	310:323	arg1	formulation					325:335	a gastroadhesive formulation	308:335	a gastroadhesive formulation to prolong taxifolin residence time	308:371	The purpose of the work was therefore to produce a gastroadhesive formulation to prolong taxifolin residence time and release in the stomach.					
31221339	5	31	theme	spherical	749:757	arg1	shape					759:763	a spherical shape	747:763	a spherical shape	747:763	Microparticles were characterized by a spherical shape and a mean volume-equivalent diameter around 12 μm.					
31221339	4	32	theme	Syloid®	626:632	arg1	silica					650:655	Syloid® AL-1 mesoporous silica	626:655	Syloid® AL-1 mesoporous silica	626:655	Next, gastromucoadhesive microparticles composed of Syloid® AL-1 mesoporous silica, chitosan and HPMC were produced using spray-drying.					
31221339	1	33	from	medium	201:206	arg1	instabile					179:187	instabile	179:187	instabile	179:187	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	1	34	theme	water	158:162	arg1	solubility					164:173	low water solubility	154:173	low water solubility	154:173	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	1	35	theme	intestinal	232:241	arg1	flora					252:256	the intestinal bacteria flora	228:256	the intestinal bacteria flora	228:256	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	4	36	theme	gastromucoadhesive	580:597	arg1	microparticles					599:612	gastromucoadhesive microparticles	580:612	gastromucoadhesive microparticles composed of Syloid® AL-1 mesoporous silica, chitosan and HPMC	580:674	Next, gastromucoadhesive microparticles composed of Syloid® AL-1 mesoporous silica, chitosan and HPMC were produced using spray-drying.					
31221339	1	37	from	instabile	179:187	arg1	medium					201:206	alkaline medium	192:206	alkaline medium	192:206	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31221339	1	38	theme	bacteria	243:250	arg1	flora					252:256	the intestinal bacteria flora	228:256	the intestinal bacteria flora	228:256	Taxifolin possesses gastroprotective property but is characterized by low water solubility, is instabile in alkaline medium, and is degraded by the intestinal bacteria flora.					
31683223	0	0	theme	growing	148:154	arg1	lambs					156:160	growing lambs	148:160	growing lambs	148:160	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	1	1	theme	barley	324:329	arg1	levels					337:342	four barley grain levels	319:342	four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM))	319:385	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	2	dep	presentation	280:291	arg1	ground					305:310	ground	305:310	ground	305:310	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	2	dep	presentation	280:291	arg1	chopped					294:300	chopped	294:300	chopped	294:300	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	0	3	from	quality	137:143	arg1	feeding					64:70	feeding behaviour	64:80	feeding behaviour	64:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	3	from	quality	137:143	arg1	diets					55:59	diets	55:59	diets on feeding behaviour	55:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	4	4	theme	ground	679:684	arg1	P < 0.01					695:702	P < 0.01	695:702	P < 0.01	695:702	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	4	4	theme	ground	679:684	arg1	alfalfa					686:692	ground alfalfa	679:692	ground alfalfa (P < 0.01)	679:703	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	0	5	from	feeding	64:70	arg1	intake					83:88	intake	83:88	intake	83:88	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	5	from	feeding	64:70	arg1	performance					116:126	animal performance	109:126	animal performance	109:126	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	5	from	feeding	64:70	arg1	quality					137:143	meat quality	132:143	meat quality	132:143	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	5	from	feeding	64:70	arg1	parameters					97:106	rumen parameters	91:106	rumen parameters	91:106	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	5	from	feeding	64:70	arg1	size					28:31	particle size	19:31	particle size	19:31	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	5	from	feeding	64:70	arg1	content					44:50	starch content	37:50	starch content	37:50	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	7	6	from	reduction	1019:1027	arg1	diet					1042:1045	diet	1042:1045	diet	1042:1045	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	6	7	theme	t11-18:1	946:953	arg1	proportions					931:941	high proportions	926:941	high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1	926:1012	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	1	8	theme	low	400:402	arg1	by-products					427:437	low starch agro-industrial by-products	400:437	low starch agro-industrial by-products (LSBP)	400:444	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	8	theme	low	400:402	arg1	LSBP					440:443	LSBP	440:443	LSBP	440:443	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	0	9	from	content	44:50	arg1	feeding					64:70	feeding behaviour	64:80	feeding behaviour	64:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	9	from	content	44:50	arg1	diets					55:59	diets	55:59	diets on feeding behaviour	55:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	10	theme	lambs	156:160	arg1	intake					83:88	intake	83:88	intake	83:88	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	10	theme	lambs	156:160	arg1	performance					116:126	animal performance	109:126	animal performance	109:126	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	10	theme	lambs	156:160	arg1	quality					137:143	meat quality	132:143	meat quality	132:143	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	10	theme	lambs	156:160	arg1	parameters					97:106	rumen parameters	91:106	rumen parameters	91:106	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	10	theme	lambs	156:160	arg1	size					28:31	particle size	19:31	particle size	19:31	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	10	theme	lambs	156:160	arg1	content					44:50	starch content	37:50	starch content	37:50	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	7	11	theme	particle	1168:1175	arg1	size					1177:1180	forage particle size	1161:1180	forage particle size	1161:1180	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	1	12	theme	starch	404:409	arg1	by-products					427:437	low starch agro-industrial by-products	400:437	low starch agro-industrial by-products (LSBP)	400:444	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	12	theme	starch	404:409	arg1	LSBP					440:443	LSBP	440:443	LSBP	440:443	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	0	13	from	performance	116:126	arg1	feeding					64:70	feeding behaviour	64:80	feeding behaviour	64:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	13	from	performance	116:126	arg1	diets					55:59	diets	55:59	diets on feeding behaviour	55:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	4	14	theme	carcass	647:653	arg1	weight					655:660	carcass weight	647:660	carcass weight	647:660	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	4	15	theme	DM	607:608	arg1	weight					655:660	carcass weight	647:660	carcass weight	647:660	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	4	15	theme	DM	607:608	arg1	Intake					597:602	Intake	597:602	Intake of DM	597:608	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	4	15	theme	DM	607:608	arg1	ADG					638:640	ADG	638:640	ADG	638:640	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	4	15	theme	DM	607:608	arg1	gain					632:635	average daily weight gain	611:635	average daily weight gain (ADG)	611:641	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	1	16	theme	agro-industrial	411:425	arg1	by-products					427:437	low starch agro-industrial by-products	400:437	low starch agro-industrial by-products (LSBP)	400:444	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	16	theme	agro-industrial	411:425	arg1	LSBP					440:443	LSBP	440:443	LSBP	440:443	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	7	17	theme	size	1177:1180	arg1	increase					1149:1156	the increase	1145:1156	the increase of forage particle size	1145:1180	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	2	18	theme	soybean	483:489	arg1	oil					491:493	6% soybean oil	480:493	6% soybean oil	480:493	The diets were supplemented with 6% soybean oil and included 40% of alfalfa in DM.					
31683223	1	19	theme	33	366:367	arg1	%					368:368	%	368:368	%	368:368	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	6	20	theme	high	926:929	arg1	proportions					931:941	high proportions	926:941	high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1	926:1012	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	0	21	from	size	28:31	arg1	feeding					64:70	feeding behaviour	64:80	feeding behaviour	64:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	21	from	size	28:31	arg1	diets					55:59	diets	55:59	diets on feeding behaviour	55:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	2	22	theme	%	481:481	arg1	oil					491:493	6% soybean oil	480:493	6% soybean oil	480:493	The diets were supplemented with 6% soybean oil and included 40% of alfalfa in DM.					
31683223	1	23	theme	%	368:368	arg1	DM					382:383	DM	382:383	DM	382:383	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	23	theme	%	368:368	arg1	matter					374:379	33% dry matter	366:379	33% dry matter (DM)	366:384	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	7	24	theme	moderate	1053:1060	arg1	impact					1071:1076	a moderate positive impact	1051:1076	a moderate positive impact	1051:1076	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	6	25	theme	c9	956:957	arg1	proportions					931:941	high proportions	926:941	high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1	926:1012	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	2	26	theme	6	480:480	arg1	%					481:481	%	481:481	%	481:481	The diets were supplemented with 6% soybean oil and included 40% of alfalfa in DM.					
31683223	1	27	theme	dry	370:372	arg1	DM					382:383	DM	382:383	DM	382:383	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	27	theme	dry	370:372	arg1	matter					374:379	33% dry matter	366:379	33% dry matter (DM)	366:384	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	7	28	theme	positive	1062:1069	arg1	impact					1071:1076	a moderate positive impact	1051:1076	a moderate positive impact	1051:1076	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	0	29	theme	particle	19:26	arg1	size					28:31	particle size	19:31	particle size	19:31	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	2	30	theme	alfalfa	515:521	arg1	alfalfa					515:521	alfalfa	515:521	alfalfa	515:521	The diets were supplemented with 6% soybean oil and included 40% of alfalfa in DM.					
31683223	2	30	theme	alfalfa	515:521	arg1	%					510:510	40%	508:510	40% of alfalfa	508:521	The diets were supplemented with 6% soybean oil and included 40% of alfalfa in DM.					
31683223	6	31	theme	ratio	992:996	arg1	proportions					931:941	high proportions	926:941	high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1	926:1012	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	0	32	theme	rumen	91:95	arg1	parameters					97:106	rumen parameters	91:106	rumen parameters	91:106	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	1	33	theme	grain	331:335	arg1	levels					337:342	four barley grain levels	319:342	four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM))	319:385	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	5	34	theme	DM	754:755	arg1	intake					757:762	DM intake	754:762	DM intake	754:762	Replacing barley grain by LSBP reduced linearly DM intake and ADG (P < 0.001) but increased meat tenderness (P = 0.046).					
31683223	1	35	theme	crossbred	174:182	arg1	branco					191:196	crossbred Merino branco	174:196	crossbred Merino branco	174:196	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	7	36	theme	barley	1032:1037	arg1	reduction					1019:1027	The reduction	1015:1027	The reduction of barley in diet	1015:1045	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	0	37	theme	animal	109:114	arg1	performance					116:126	animal performance	109:126	animal performance	109:126	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	5	38	theme	meat	798:801	arg1	tenderness					803:812	meat tenderness	798:812	meat tenderness (P = 0.046)	798:824	Replacing barley grain by LSBP reduced linearly DM intake and ADG (P < 0.001) but increased meat tenderness (P = 0.046).					
31683223	5	38	theme	meat	798:801	arg1	P = 0.046					815:823	P = 0.046	815:823	P = 0.046	815:823	Replacing barley grain by LSBP reduced linearly DM intake and ADG (P < 0.001) but increased meat tenderness (P = 0.046).					
31683223	1	39	theme	Merino	184:189	arg1	branco					191:196	crossbred Merino branco	174:196	crossbred Merino branco	174:196	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	7	40	dep	size	1177:1180	arg1	forage					1161:1166	forage	1161:1166	forage	1161:1166	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	0	41	from	diets	55:59	arg1	feeding					64:70	feeding behaviour	64:80	feeding behaviour	64:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	6	42	theme	t10-18:1/t11-18:1	974:990	arg1	ratio					992:996	a t10-18:1/t11-18:1 ratio	972:996	a t10-18:1/t11-18:1 ratio clearly below 1	972:1012	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	1	43	theme	alfalfa	272:278	arg1	presentation					280:291	alfalfa presentation	272:291	alfalfa presentation (chopped or ground)	272:311	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	7	44	theme	meat	1081:1084	arg1	composition					1089:1099	meat FA composition	1081:1099	meat FA composition	1081:1099	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	7	45	contain	had	1047:1049	arg1	reduction					1019:1027	The reduction	1015:1027	The reduction of barley in diet	1015:1045	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	7	45	contain	had	1047:1049	arg2	impact					1071:1076	a moderate positive impact	1051:1076	a moderate positive impact	1051:1076	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	6	46	theme	FA	908:909	arg1	profile					912:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	0	47	theme	starch	37:42	arg1	content					44:50	starch content	37:50	starch content	37:50	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	1	48	theme	presentation	280:291	arg1	forms					263:267	two forms	259:267	two forms of alfalfa presentation (chopped or ground)	259:311	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	5	49	theme	barley	716:721	arg1	grain					723:727	barley grain	716:727	barley grain	716:727	Replacing barley grain by LSBP reduced linearly DM intake and ADG (P < 0.001) but increased meat tenderness (P = 0.046).					
31683223	7	50	theme	FA	1086:1087	arg1	composition					1089:1099	meat FA composition	1081:1099	meat FA composition	1081:1099	The reduction of barley in diet had a moderate positive impact on meat FA composition, decreasing t10-18:1, which was enhanced by the increase of forage particle size.					
31683223	0	51	from	intake	83:88	arg1	feeding					64:70	feeding behaviour	64:80	feeding behaviour	64:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	51	from	intake	83:88	arg1	diets					55:59	diets	55:59	diets on feeding behaviour	55:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	4	52	theme	average	611:617	arg1	ADG					638:640	ADG	638:640	ADG	638:640	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	4	52	theme	average	611:617	arg1	gain					632:635	average daily weight gain	611:635	average daily weight gain (ADG)	611:641	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	6	53	theme	acid	902:905	arg1	profile					912:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	1	54	theme	male	198:201	arg1	lambs					203:207	Thirty-two crossbred Merino branco male lambs	163:207	Thirty-two crossbred Merino branco male lambs	163:207	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	0	55	theme	meat	132:135	arg1	quality					137:143	meat quality	132:143	meat quality	132:143	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	6	56	theme	fatty	896:900	arg1	profile					912:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	6	57	theme	healthy	871:877	arg1	profile					912:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	4	58	theme	weight	625:630	arg1	ADG					638:640	ADG	638:640	ADG	638:640	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	4	58	theme	weight	625:630	arg1	gain					632:635	average daily weight gain	611:635	average daily weight gain (ADG)	611:641	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	6	59	theme	biohydrogenation	879:894	arg1	profile					912:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	a healthy biohydrogenation fatty acid (FA) profile	869:918	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	0	60	from	parameters	97:106	arg1	feeding					64:70	feeding behaviour	64:80	feeding behaviour	64:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	0	60	from	parameters	97:106	arg1	diets					55:59	diets	55:59	diets on feeding behaviour	55:80	Effects of alfalfa particle size and starch content in diets on feeding behaviour, intake, rumen parameters, animal performance and meat quality of growing lambs.					
31683223	4	61	theme	daily	619:623	arg1	ADG					638:640	ADG	638:640	ADG	638:640	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	4	61	theme	daily	619:623	arg1	gain					632:635	average daily weight gain	611:635	average daily weight gain (ADG)	611:641	Intake of DM, average daily weight gain (ADG) and carcass weight were higher with ground alfalfa (P < 0.01).					
31683223	6	62	theme	t11-18:2	959:966	arg1	proportions					931:941	high proportions	926:941	high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1	926:1012	Regardless of the treatments, meat showed a healthy biohydrogenation fatty acid (FA) profile, with high proportions of t11-18:1, c9,t11-18:2 and a t10-18:1/t11-18:1 ratio clearly below 1.					
31683223	1	63	dep	levels	337:342	arg1	%					360:360	21.3%	356:360	21.3%	356:360	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	63	dep	levels	337:342	arg1	matter					374:379	33% dry matter	366:379	33% dry matter (DM)	366:384	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	63	dep	levels	337:342	arg1	DM					382:383	DM	382:383	DM	382:383	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	63	dep	levels	337:342	arg1	%					346:346	0%	345:346	0%	345:346	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
31683223	1	63	dep	levels	337:342	arg1	%					353:353	11.2%	349:353	11.2%	349:353	Thirty-two crossbred Merino branco male lambs were randomly allocated to eight diets, combining two forms of alfalfa presentation (chopped or ground), with four barley grain levels (0%, 11.2%, 21.3% and 33% dry matter (DM)), balanced by low starch agro-industrial by-products (LSBP).					
30463326	8	0	theme	mL	1286:1287	arg1	TRS					1324:1326	0.6 mol H₂/mol TRS	1309:1326	0.6 mol H₂/mol TRS	1309:1326	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	8	0	theme	mL	1286:1287	arg1	poplar					1301:1306	22.99 mL H₂/g energy poplar	1280:1306	22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1280:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	8	1	theme	Enterobacter	1223:1234	arg1	aerogenes					1236:1244	Enterobacter aerogenes ATCC 13048	1223:1255	Enterobacter aerogenes ATCC 13048	1223:1255	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	1	2	theme	hard	266:269	arg1	wood					271:274	hard wood	266:274	hard wood	266:274	The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products.					
30463326	0	3	theme	Enzymatic	73:81	arg1	Hydrolysis					83:92	Enzymatic Hydrolysis	73:92	Enzymatic Hydrolysis	73:92	Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis.					
30463326	4	4	dep	sugars	787:792	arg1	degree					810:815	degree	810:815	degree of lignin removal	810:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	4	dep	sugars	787:792	arg1	yield					800:804	yield	800:804	yield	800:804	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	4	dep	sugars	787:792	arg1	sugars					787:792	both total released sugars	767:792	both total released sugars (TRS) yield and degree of lignin removal	767:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	1	5	theme	wood	271:274	arg1	processing					252:261	the processing	248:261	the processing of hard wood towards useful products	248:298	The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products.					
30463326	3	6	theme	immobilized	559:569	arg1	enzymes					575:581	diatomite immobilized bed enzymes	549:581	diatomite immobilized bed enzymes	549:581	Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification.					
30463326	4	7	theme	response	622:629	arg1	methodology					639:649	response surface methodology	622:649	The response surface methodology (RSM) method	618:662	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	7	theme	response	622:629	arg1	RSM					652:654	RSM	652:654	RSM	652:654	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	8	8	theme	dark	1174:1177	arg1	fermentation					1179:1190	dark fermentation	1174:1190	dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1174:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	4	9	theme	sugars	787:792	arg1	values					757:762	the highest values	745:762	the highest values of both total released sugars (TRS) yield and degree of lignin removal	745:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	3	10	theme	bed	571:573	arg1	enzymes					575:581	diatomite immobilized bed enzymes	549:581	diatomite immobilized bed enzymes	549:581	Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification.					
30463326	1	11	theme	useful	284:289	arg1	products					291:298	useful products	284:298	useful products	284:298	The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products.					
30463326	8	12	theme	batch	1195:1199	arg1	reactors					1201:1208	batch reactors	1195:1208	batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1195:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	7	13	theme	enzymatic	1097:1105	arg1	reagents					1107:1114	both alkaline as well as enzymatic reagents	1072:1114	both alkaline as well as enzymatic reagents	1072:1114	Additionally, the possibility of the re-use of both alkaline as well as enzymatic reagents was investigated.					
30463326	8	14	theme	22.99	1280:1284	arg1	mL					1286:1287	mL	1286:1287	mL	1286:1287	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	8	15	theme	final	1264:1268	arg1	result					1270:1275	a final result	1262:1275	a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1262:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	7	16	theme	reagents	1107:1114	arg1	re-use					1062:1067	the re-use	1058:1067	the re-use of both alkaline as well as enzymatic reagents	1058:1114	Additionally, the possibility of the re-use of both alkaline as well as enzymatic reagents was investigated.					
30463326	3	17	theme	diatomite	549:557	arg1	enzymes					575:581	diatomite immobilized bed enzymes	549:581	diatomite immobilized bed enzymes	549:581	Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification.					
30463326	2	18	theme	alkaline	391:398	arg1	pre-treatment					400:412	alkaline pre-treatment	391:412	alkaline pre-treatment	391:412	Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied.					
30463326	0	19	from	Poplar	32:37	arg1	Production					9:18	Hydrogen Production	0:18	Hydrogen Production from Energy Poplar	0:37	Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis.					
30463326	5	20	theme	pre-treatment	940:952	arg1	conditions					954:963	the alkaline pre-treatment conditions	927:963	the alkaline pre-treatment conditions	927:963	Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions.					
30463326	4	21	theme	optimal	690:696	arg1	conditions					721:730	the optimal alkaline pre-treatment conditions	686:730	the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal	686:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	0	22	theme	Hydrogen	0:7	arg1	Production					9:18	Hydrogen Production	0:18	Hydrogen Production from Energy Poplar	0:37	Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis.					
30463326	4	23	theme	highest	749:755	arg1	values					757:762	the highest values	745:762	the highest values of both total released sugars (TRS) yield and degree of lignin removal	745:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	0	24	theme	Energy	25:30	arg1	Poplar					32:37	Energy Poplar	25:37	Energy Poplar	25:37	Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis.					
30463326	7	25	theme	alkaline	1077:1084	arg1	reagents					1107:1114	both alkaline as well as enzymatic reagents	1072:1114	both alkaline as well as enzymatic reagents	1072:1114	Additionally, the possibility of the re-use of both alkaline as well as enzymatic reagents was investigated.					
30463326	5	26	theme	alkaline	931:938	arg1	conditions					954:963	the alkaline pre-treatment conditions	927:963	the alkaline pre-treatment conditions	927:963	Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions.					
30463326	4	27	theme	released	778:785	arg1	degree					810:815	degree	810:815	degree of lignin removal	810:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	27	theme	released	778:785	arg1	yield					800:804	yield	800:804	yield	800:804	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	27	theme	released	778:785	arg1	TRS					795:797	TRS	795:797	TRS	795:797	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	27	theme	released	778:785	arg1	sugars					787:792	both total released sugars	767:792	both total released sugars (TRS) yield and degree of lignin removal	767:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	5	28	theme	variable	842:849	arg1	parameters					851:860	Three variable parameters	836:860	Three variable parameters (temperature, MEA concentration, time)	836:899	Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions.					
30463326	2	29	theme	poplar	373:378	arg1	pre-treatment					349:361	the pre-treatment	345:361	the pre-treatment of energy poplar	345:378	Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied.					
30463326	4	30	theme	total	772:776	arg1	degree					810:815	degree	810:815	degree of lignin removal	810:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	30	theme	total	772:776	arg1	yield					800:804	yield	800:804	yield	800:804	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	30	theme	total	772:776	arg1	TRS					795:797	TRS	795:797	TRS	795:797	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	30	theme	total	772:776	arg1	sugars					787:792	both total released sugars	767:792	both total released sugars (TRS) yield and degree of lignin removal	767:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	1	31	theme	lignocellulose	210:223	arg1	composition					195:205	the composition	191:205	the composition of lignocellulose	191:223	The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products.					
30463326	8	32	theme	ATCC	1246:1249	arg1	13048					1251:1255	ATCC 13048	1246:1255	Enterobacter aerogenes ATCC 13048	1223:1255	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	8	33	theme	poplar	1301:1306	arg1	result					1270:1275	a final result	1262:1275	a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1262:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	8	34	theme	energy	1294:1299	arg1	TRS					1324:1326	0.6 mol H₂/mol TRS	1309:1326	0.6 mol H₂/mol TRS	1309:1326	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	8	34	theme	energy	1294:1299	arg1	poplar					1301:1306	22.99 mL H₂/g energy poplar	1280:1306	22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1280:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	4	35	theme	removal	827:833	arg1	degree					810:815	degree	810:815	degree of lignin removal	810:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	35	theme	removal	827:833	arg1	yield					800:804	yield	800:804	yield	800:804	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	35	theme	removal	827:833	arg1	sugars					787:792	both total released sugars	767:792	both total released sugars (TRS) yield and degree of lignin removal	767:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	2	36	theme	enzymatic	418:426	arg1	saccharification					428:443	enzymatic saccharification	418:443	enzymatic saccharification	418:443	Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied.					
30463326	4	37	theme	pre-treatment	707:719	arg1	conditions					721:730	the optimal alkaline pre-treatment conditions	686:730	the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal	686:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	6	38	theme	Box-Behnken	1005:1015	arg1	design					1017:1022	the Box-Behnken design	1001:1022	the Box-Behnken design	1001:1022	The research was carried out using the Box-Behnken design.					
30463326	8	39	theme	H₂/mol	1317:1322	arg1	TRS					1324:1326	0.6 mol H₂/mol TRS	1309:1326	0.6 mol H₂/mol TRS	1309:1326	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	8	39	theme	H₂/mol	1317:1322	arg1	poplar					1301:1306	22.99 mL H₂/g energy poplar	1280:1306	22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1280:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	4	40	theme	lignin	820:825	arg1	removal					827:833	lignin removal	820:833	lignin removal	820:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	3	41	used	used	588:591	arg2	enzymes					575:581	diatomite immobilized bed enzymes	549:581	diatomite immobilized bed enzymes	549:581	Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification.					
30463326	0	42	theme	MEA	51:53	arg1	Pre-Treatment					55:67	MEA Pre-Treatment	51:67	MEA Pre-Treatment	51:67	Hydrogen Production from Energy Poplar Preceded by MEA Pre-Treatment and Enzymatic Hydrolysis.					
30463326	3	43	used	used	515:518	arg2	catalyst					536:543	the alkaline catalyst	523:543	the alkaline catalyst	523:543	Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification.					
30463326	3	43	used	used	515:518	arg2	MEA					506:508	MEA	506:508	MEA	506:508	Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification.					
30463326	3	43	used	used	515:518	arg2	Monoethanolamine					488:503	Monoethanolamine	488:503	Monoethanolamine (MEA)	488:509	Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification.					
30463326	1	44	theme	pre-treat	107:115	arg1	biomass					133:139	pre-treat lignocellulosic biomass	107:139	pre-treat lignocellulosic biomass	107:139	The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products.					
30463326	8	45	dep	aerogenes	1236:1244	arg1	13048					1251:1255	ATCC 13048	1246:1255	Enterobacter aerogenes ATCC 13048	1223:1255	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	7	46	theme	re-use	1062:1067	arg1	possibility					1043:1053	the possibility	1039:1053	the possibility of the re-use of both alkaline as well as enzymatic reagents	1039:1114	Additionally, the possibility of the re-use of both alkaline as well as enzymatic reagents was investigated.					
30463326	1	47	theme	dark	150:153	arg1	fermentation					155:166	dark fermentation	150:166	dark fermentation	150:166	The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products.					
30463326	1	48	theme	lignocellulosic	117:131	arg1	biomass					133:139	pre-treat lignocellulosic biomass	107:139	pre-treat lignocellulosic biomass	107:139	The need to pre-treat lignocellulosic biomass prior to dark fermentation results primarily from the composition of lignocellulose because lignin hinders the processing of hard wood towards useful products.					
30463326	8	49	theme	Obtained	1134:1141	arg1	hydrolysates					1143:1154	Obtained hydrolysates	1134:1154	Obtained hydrolysates	1134:1154	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	4	50	theme	alkaline	698:705	arg1	conditions					721:730	the optimal alkaline pre-treatment conditions	686:730	the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal	686:833	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	8	51	theme	mol	1313:1315	arg1	TRS					1324:1326	0.6 mol H₂/mol TRS	1309:1326	0.6 mol H₂/mol TRS	1309:1326	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	8	51	theme	mol	1313:1315	arg1	poplar					1301:1306	22.99 mL H₂/g energy poplar	1280:1306	22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1280:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	4	52	theme	methodology	639:649	arg1	method					657:662	The response surface methodology (RSM) method	618:662	The response surface methodology (RSM) method	618:662	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	8	53	from	fermentation	1179:1190	arg1	reactors					1201:1208	batch reactors	1195:1208	batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1195:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	2	54	theme	two-step	323:330	arg1	approach					332:339	a two-step approach	321:339	a two-step approach	321:339	Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied.					
30463326	4	55	theme	surface	631:637	arg1	methodology					639:649	response surface methodology	622:649	The response surface methodology (RSM) method	618:662	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	4	55	theme	surface	631:637	arg1	RSM					652:654	RSM	652:654	RSM	652:654	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	8	56	theme	H₂/g	1289:1292	arg1	TRS					1324:1326	0.6 mol H₂/mol TRS	1309:1326	0.6 mol H₂/mol TRS	1309:1326	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	8	56	theme	H₂/g	1289:1292	arg1	poplar					1301:1306	22.99 mL H₂/g energy poplar	1280:1306	22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS)	1280:1327	Obtained hydrolysates were subjected to dark fermentation in batch reactors performed by Enterobacter aerogenes ATCC 13048 with a final result of 22.99 mL H₂/g energy poplar (0.6 mol H₂/mol TRS).					
30463326	4	57	used	used	668:671	arg2	method					657:662	The response surface methodology (RSM) method	618:662	The response surface methodology (RSM) method	618:662	The response surface methodology (RSM) method was used to determine the optimal alkaline pre-treatment conditions resulting in the highest values of both total released sugars (TRS) yield and degree of lignin removal.					
30463326	3	58	theme	alkaline	527:534	arg1	Monoethanolamine					488:503	Monoethanolamine	488:503	Monoethanolamine (MEA)	488:509	Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification.					
30463326	3	58	theme	alkaline	527:534	arg1	catalyst					536:543	the alkaline catalyst	523:543	the alkaline catalyst	523:543	Monoethanolamine (MEA) was used as the alkaline catalyst and diatomite immobilized bed enzymes were used during saccharification.					
30463326	5	59	theme	MEA	876:878	arg1	temperature					863:873	temperature	863:873	temperature	863:873	Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions.					
30463326	5	59	theme	MEA	876:878	arg1	concentration					880:892	MEA concentration	876:892	MEA concentration	876:892	Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions.					
30463326	5	60	dep	parameters	851:860	arg1	time					895:898	time	895:898	time	895:898	Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions.					
30463326	5	60	dep	parameters	851:860	arg1	temperature					863:873	temperature	863:873	temperature	863:873	Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions.					
30463326	5	60	dep	parameters	851:860	arg1	concentration					880:892	MEA concentration	876:892	MEA concentration	876:892	Three variable parameters (temperature, MEA concentration, time) were selected to optimize the alkaline pre-treatment conditions.					
30463326	2	61	theme	energy	366:371	arg1	poplar					373:378	energy poplar	366:378	energy poplar	366:378	Hence, in this work a two-step approach for the pre-treatment of energy poplar, including alkaline pre-treatment and enzymatic saccharification followed by fermentation has been studied.					
30126191	5	0	theme	aureus	809:814	arg1	bacteria					816:823	Gram-positive Staphylococcus aureus bacteria	780:823	Gram-positive Staphylococcus aureus bacteria	780:823	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	0	1	from	Immobilization	21:34	arg1	Membranes					52:60	New Chitosan Membranes	39:60	New Chitosan Membranes	39:60	Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.					
30126191	7	2	theme	promising	1123:1131	arg1	performance					1133:1143	promising performance	1123:1143	promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions	1123:1252	The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.					
30126191	0	3	theme	Biomedical	76:85	arg1	Applications					87:98	Biomedical Applications	76:98	Biomedical Applications	76:98	Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.					
30126191	6	4	theme	Mg2+	971:974	arg1	incorporation					954:966	the incorporation	950:966	the incorporation of Mg2+	950:974	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+.					
30126191	5	5	theme	Mg2+	665:668	arg1	effect					655:660	the effect	651:660	the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior	651:865	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	1	6	attach	present	119:125	arg2	we					116:117	we	116:117	we	116:117	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	1	6	attach	present	119:125	arg1	paper					109:113	this paper	104:113	this paper	104:113	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	2	7	theme	metal	298:302	arg1	ions					304:307	metal ions	298:307	metal ions	298:307	This technology involved metal ions incorporation to develop new biopolymeric supports.					
30126191	4	8	theme	ion	620:622	arg1	release					624:630	the metal ion release	610:630	the metal ion release in time	610:638	The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time.					
30126191	2	9	theme	new	334:336	arg1	biopolymeric					338:349	new biopolymeric supports	334:358	new biopolymeric supports	334:358	This technology involved metal ions incorporation to develop new biopolymeric supports.					
30126191	4	10	theme	metal	614:618	arg1	release					624:630	the metal ion release	610:630	the metal ion release in time	610:638	The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time.					
30126191	1	11	theme	membranes	186:194	arg1	characterization					147:162	characterization	147:162	characterization	147:162	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	1	11	theme	membranes	186:194	arg1	fabrication					131:141	fabrication	131:141	fabrication	131:141	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	1	12	theme	new	167:169	arg1	membranes					186:194	new chitosan-based membranes	167:194	new chitosan-based membranes	167:194	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	3	13	theme	chemical	365:372	arg1	structure					374:382	chemical structure	365:382	chemical structure	365:382	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	3	14	theme	proposed	421:428	arg1	membranes					430:438	proposed membranes	421:438	proposed membranes	421:438	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	7	15	dep	biopolymeric	1184:1195	arg1	supports					1197:1204	supports	1197:1204	supports	1197:1204	The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.					
30126191	1	16	theme	chitosan-based	171:184	arg1	membranes					186:194	new chitosan-based membranes	167:194	new chitosan-based membranes	167:194	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	0	17	theme	Phosphatase	9:19	arg1	Immobilization					21:34	Alkaline Phosphatase Immobilization	0:34	Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.	0:99	Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.					
30126191	4	18	from	release	624:630	arg1	time					635:638	time	635:638	time	635:638	The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time.					
30126191	2	19	dep	biopolymeric	338:349	arg1	supports					351:358	supports	351:358	supports	351:358	This technology involved metal ions incorporation to develop new biopolymeric supports.					
30126191	3	20	theme	infrared	458:465	arg1	FT-IR					481:485	FT-IR	481:485	FT-IR	481:485	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	3	20	theme	infrared	458:465	arg1	spectroscopy					467:478	infrared spectroscopy	458:478	infrared spectroscopy (FT-IR)	458:486	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	0	21	theme	Alkaline	0:7	arg1	Immobilization					21:34	Alkaline Phosphatase Immobilization	0:34	Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.	0:99	Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.					
30126191	6	22	with	polymeric	983:991	arg1	viability					1025:1033	enhanced cellular viability	1007:1033	enhanced cellular viability	1007:1033	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+.					
30126191	6	23	dep	polymeric	983:991	arg1	supports					993:1000	supports	993:1000	supports	993:1000	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+.					
30126191	3	24	theme	morphological	388:400	arg1	characteristics					402:416	morphological characteristics	388:416	morphological characteristics	388:416	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	5	25	from	effect	655:660	arg1	investigations					718:731	the antibacterial investigations	700:731	the antibacterial investigations	700:731	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	5	25	from	effect	655:660	arg1	behavior					858:865	biocompatibility behavior	841:865	biocompatibility behavior	841:865	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	5	25	from	effect	655:660	arg1	activity					687:694	the enzymatic activity	673:694	the enzymatic activity	673:694	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	5	25	from	effect	655:660	arg1	hemolysis					826:834	hemolysis	826:834	hemolysis	826:834	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	5	26	theme	antibacterial	704:716	arg1	investigations					718:731	the antibacterial investigations	700:731	the antibacterial investigations	700:731	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	1	27	dep	fabrication	131:141	arg1	the					127:129	the	127:129	the	127:129	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	7	28	theme	metal	1243:1247	arg1	ions					1249:1252	metal ions	1243:1252	metal ions	1243:1252	The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.					
30126191	3	29	theme	scanning	496:503	arg1	microscopy					514:523	scanning electron microscopy	496:523	the scanning electron microscopy technique (SEM)	492:539	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	0	30	theme	Chitosan	43:50	arg1	Membranes					52:60	New Chitosan Membranes	39:60	New Chitosan Membranes	39:60	Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.					
30126191	6	31	theme	cellular	1016:1023	arg1	viability					1025:1033	enhanced cellular viability	1007:1033	enhanced cellular viability	1007:1033	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+.					
30126191	6	32	theme	membranes	916:924	arg1	composition					926:936	the chitosan membranes composition	903:936	the chitosan membranes composition followed by the incorporation of Mg2+	903:974	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+.					
30126191	1	33	theme	new	210:212	arg1	biotechnology					214:226	a new biotechnology	208:226	a new biotechnology for immobilizing alkaline phosphatase (ALP)	208:270	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	0	34	theme	New	39:41	arg1	Membranes					52:60	New Chitosan Membranes	39:60	New Chitosan Membranes	39:60	Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.					
30126191	4	35	theme	coupled	558:564	arg1	ICP-MS					592:597	ICP-MS	592:597	ICP-MS	592:597	The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time.					
30126191	4	35	theme	coupled	558:564	arg1	spectrometry					578:589	The inductively coupled plasma mass spectrometry	542:589	The inductively coupled plasma mass spectrometry (ICP-MS)	542:598	The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time.					
30126191	7	36	theme	biomedical	1148:1157	arg1	applications					1159:1170	biomedical applications	1148:1170	biomedical applications	1148:1170	The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.					
30126191	6	37	theme	chitosan	907:914	arg1	membranes					916:924	chitosan membranes	907:924	the chitosan membranes composition followed by the incorporation of Mg2+	903:974	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+.					
30126191	5	38	dep	activity	687:694	arg1	using					739:743	using	739:743	using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria	739:823	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	3	39	theme	membranes	430:438	arg1	structure					374:382	chemical structure	365:382	chemical structure	365:382	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	3	39	theme	membranes	430:438	arg1	characteristics					402:416	morphological characteristics	388:416	morphological characteristics	388:416	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	6	40	theme	enhanced	1007:1014	arg1	viability					1025:1033	enhanced cellular viability	1007:1033	enhanced cellular viability	1007:1033	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+.					
30126191	5	41	theme	enzymatic	677:685	arg1	activity					687:694	the enzymatic activity	673:694	the enzymatic activity	673:694	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	0	42	with	Immobilization	21:34	arg1	Mg2+					67:70	Mg2+	67:70	Mg2+ for Biomedical Applications	67:98	Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications.					
30126191	4	43	theme	mass	573:576	arg1	ICP-MS					592:597	ICP-MS	592:597	ICP-MS	592:597	The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time.					
30126191	4	43	theme	mass	573:576	arg1	spectrometry					578:589	The inductively coupled plasma mass spectrometry	542:589	The inductively coupled plasma mass spectrometry (ICP-MS)	542:598	The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time.					
30126191	4	44	theme	plasma	566:571	arg1	ICP-MS					592:597	ICP-MS	592:597	ICP-MS	592:597	The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time.					
30126191	4	44	theme	plasma	566:571	arg1	spectrometry					578:589	The inductively coupled plasma mass spectrometry	542:589	The inductively coupled plasma mass spectrometry (ICP-MS)	542:598	The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time.					
30126191	3	45	dep	structure	374:382	arg1	The					361:363	The	361:363	The	361:363	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	3	46	theme	electron	505:512	arg1	microscopy					514:523	scanning electron microscopy	496:523	the scanning electron microscopy technique (SEM)	492:539	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	5	47	dep	Escherichia	759:769	arg1	coli					771:774	coli	771:774	coli	771:774	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	6	48	theme	Immobilizing	881:892	arg1	ALP					894:896	Immobilizing ALP	881:896	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+	881:974	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+.					
30126191	3	49	theme	microscopy	514:523	arg1	technique					525:533	the scanning electron microscopy technique	492:533	the scanning electron microscopy technique (SEM)	492:539	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	3	49	theme	microscopy	514:523	arg1	SEM					536:538	SEM	536:538	SEM	536:538	The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM).					
30126191	5	50	theme	Gram-positive	780:792	arg1	bacteria					816:823	Gram-positive Staphylococcus aureus bacteria	780:823	Gram-positive Staphylococcus aureus bacteria	780:823	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	1	51	theme	alkaline	245:252	arg1	ALP					267:269	ALP	267:269	ALP	267:269	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	1	51	theme	alkaline	245:252	arg1	phosphatase					254:264	alkaline phosphatase	245:264	alkaline phosphatase (ALP)	245:270	In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP).					
30126191	6	52	theme	chitosan-based	1053:1066	arg1	membranes					1068:1076	chitosan-based membranes	1053:1076	chitosan-based membranes without Mg2+	1053:1089	Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+.					
30126191	5	53	theme	biocompatibility	841:856	arg1	behavior					858:865	biocompatibility behavior	841:865	biocompatibility behavior	841:865	Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied.					
30126191	7	54	from	performance	1133:1143	arg1	applications					1159:1170	biomedical applications	1148:1170	biomedical applications	1148:1170	The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.					
30847541	1	0	used	used	296:299	arg2	ability					139:145	The ability	135:145	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time	135:290	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	3	1	theme	rumen	473:477	arg1	consortium					527:536	the converting microbial consortium	502:536	the converting microbial consortium	502:536	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	3	1	theme	rumen	473:477	arg1	microbiome					479:488	The rumen microbiome	469:488	The rumen microbiome	469:488	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	1	2	theme	aerobic	244:250	arg1	microbes					266:273	aerobic and anaerobic microbes	244:273	aerobic and anaerobic microbes	244:273	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	6	3	theme	chain	1145:1149	arg1	acids					1157:1161	the short chain fatty acids	1135:1161	the short chain fatty acids produced	1135:1170	Fermentation temperature was crucial especially for the composition of the short chain fatty acids produced.					
30847541	3	4	theme	converting	506:515	arg1	consortium					527:536	the converting microbial consortium	502:536	the converting microbial consortium	502:536	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	3	4	theme	converting	506:515	arg1	microbiome					479:488	The rumen microbiome	469:488	The rumen microbiome	469:488	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	1	5	theme	anaerobic	256:264	arg1	microbes					266:273	aerobic and anaerobic microbes	244:273	aerobic and anaerobic microbes	244:273	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	1	6	theme	biofilm	167:173	arg1	reactors					184:191	the multispecies biofilm membrane reactors	150:191	the multispecies biofilm membrane reactors (MBM reactors)	150:206	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	1	6	theme	biofilm	167:173	arg1	reactors					198:205	MBM reactors	194:205	MBM reactors	194:205	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	4	7	theme	rumen	836:840	arg1	microbiome					842:851	the rumen microbiome	832:851	the rumen microbiome alone	832:857	The beneficial effect of the fungal biofilm on the process yields and productivities was attributed to the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone.					
30847541	3	8	theme	co-cultivated	539:551	arg1	consortium					527:536	the converting microbial consortium	502:536	the converting microbial consortium	502:536	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	3	8	theme	co-cultivated	539:551	arg1	microbiome					479:488	The rumen microbiome	469:488	The rumen microbiome	469:488	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	1	9	theme	reactors	184:191	arg1	ability					139:145	The ability	135:145	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time	135:290	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	9	10	theme	organic	1623:1629	arg1	acids					1631:1635	organic acids	1623:1635	organic acids produced	1623:1644	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	1	11	theme	MBM	194:196	arg1	reactors					184:191	the multispecies biofilm membrane reactors	150:191	the multispecies biofilm membrane reactors (MBM reactors)	150:206	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	1	11	theme	MBM	194:196	arg1	reactors					198:205	MBM reactors	194:205	MBM reactors	194:205	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	5	12	theme	crystalline	1024:1034	arg1	substrate					1053:1061	the substrate	1049:1061	the substrate	1049:1061	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	5	12	theme	crystalline	1024:1034	arg1	cellulose					1036:1044	15 g/L crystalline cellulose	1017:1044	15 g/L crystalline cellulose	1017:1044	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	3	13	theme	aerobic	578:584	arg1	fungi					586:590	selected individual aerobic fungi	558:590	selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen	558:657	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	5	14	dep	higher	945:950	arg1	SCFAs					961:965	7.3 g/L SCFAs	953:965	7.3 g/L SCFAs	953:965	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	3	15	theme	selected	558:565	arg1	fungi					586:590	selected individual aerobic fungi	558:590	selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen	558:657	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	4	16	dep	process	711:717	arg1	yields					719:724	yields	719:724	yields	719:724	The beneficial effect of the fungal biofilm on the process yields and productivities was attributed to the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone.					
30847541	4	17	theme	biofilm	696:702	arg1	effect					675:680	The beneficial effect	660:680	The beneficial effect of the fungal biofilm on the process yields and productivities	660:743	The beneficial effect of the fungal biofilm on the process yields and productivities was attributed to the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone.					
30847541	5	18	theme	reesei	902:907	arg1	biofilm					909:915	Trichoderma reesei biofilm	890:915	Trichoderma reesei biofilm	890:915	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	9	19	theme	total	1671:1675	arg1	SCFAs					1677:1681	total SCFAs	1671:1681	total SCFAs	1671:1681	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	6	20	theme	acids	1157:1161	arg1	composition					1120:1130	the composition	1116:1130	the composition of the short chain fatty acids produced	1116:1170	Fermentation temperature was crucial especially for the composition of the short chain fatty acids produced.					
30847541	3	21	with	co-cultivated	539:551	arg1	fungi					586:590	selected individual aerobic fungi	558:590	selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen	558:657	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	7	22	theme	temperature	1177:1187	arg1	increase					1189:1196	The temperature increase	1173:1196	The temperature increase	1173:1196	The temperature increase resulted in shorter fatty acids produced.					
30847541	5	23	theme	MBM	874:876	arg1	system					878:883	the MBM system	870:883	the MBM system with Trichoderma reesei biofilm	870:915	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	8	24	theme	caproic	1291:1297	arg1	acids					1299:1303	acetic, propionic, butyric, and caproic acids	1259:1303	acetic, propionic, butyric, and caproic acids	1259:1303	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	0	25	theme	chain	92:96	arg1	acids					104:108	short chain fatty acids	86:108	short chain fatty acids	86:108	A cellulolytic fungal biofilm enhances the consolidated bioconversion of cellulose to short chain fatty acids by the rumen microbiome.					
30847541	1	26	theme	consolidated	330:341	arg1	bioprocessing					343:355	the consolidated bioprocessing	326:355	the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs)	326:403	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	4	27	theme	fungal	689:694	arg1	biofilm					696:702	the fungal biofilm	685:702	the fungal biofilm	685:702	The beneficial effect of the fungal biofilm on the process yields and productivities was attributed to the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone.					
30847541	9	28	dep	reached	1653:1659	arg1	30 °C					1706:1710	30 °C	1706:1710	30 °C	1706:1710	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	9	28	dep	reached	1653:1659	arg1	pH 6					1719:1722	pH 6	1719:1722	pH 6	1719:1722	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	2	29	theme	based	419:423	arg1	bioprocess					438:447	A consortium based consolidated bioprocess	406:447	A consortium based consolidated bioprocess (CBP)	406:453	A consortium based consolidated bioprocess (CBP) was designed.					
30847541	2	29	theme	based	419:423	arg1	CBP					450:452	CBP	450:452	CBP	450:452	A consortium based consolidated bioprocess (CBP) was designed.					
30847541	5	30	with	system	878:883	arg1	biofilm					909:915	Trichoderma reesei biofilm	890:915	Trichoderma reesei biofilm	890:915	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	0	31	theme	fungal	15:20	arg1	biofilm					22:28	A cellulolytic fungal biofilm	0:28	A cellulolytic fungal biofilm	0:28	A cellulolytic fungal biofilm enhances the consolidated bioconversion of cellulose to short chain fatty acids by the rumen microbiome.					
30847541	3	32	theme	tubular	622:628	arg1	membrane					630:637	the tubular membrane	618:637	the tubular membrane flushed with oxygen	618:657	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	8	33	theme	reesei	1344:1349	arg1	biofilm					1351:1357	Trichoderma reesei biofilm	1332:1357	Trichoderma reesei biofilm	1332:1357	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	9	34	theme	fungal	1539:1544	arg1	biofilm					1546:1552	the fungal biofilm	1535:1552	the fungal biofilm	1535:1552	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	0	35	theme	consolidated	43:54	arg1	bioconversion					56:68	the consolidated bioconversion	39:68	the consolidated bioconversion of cellulose to short chain fatty acids	39:108	A cellulolytic fungal biofilm enhances the consolidated bioconversion of cellulose to short chain fatty acids by the rumen microbiome.					
30847541	6	36	theme	Fermentation	1064:1075	arg1	temperature					1077:1087	Fermentation temperature	1064:1087	Fermentation temperature	1064:1087	Fermentation temperature was crucial especially for the composition of the short chain fatty acids produced.					
30847541	9	37	theme	volume	1754:1759	arg1	ratio					1761:1765	rumen inoculum to total volume ratio	1730:1765	rumen inoculum to total volume ratio equal to 0.372	1730:1780	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	4	38	theme	cellulolytic	776:787	arg1	activities					789:798	the enhanced cellulolytic activities	763:798	the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone	763:857	The beneficial effect of the fungal biofilm on the process yields and productivities was attributed to the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone.					
30847541	1	39	theme	distinguished	219:231	arg1	niches					233:238	distinguished niches	219:238	distinguished niches for aerobic and anaerobic microbes	219:273	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	9	40	theme	equal	1767:1771	arg1	ratio					1761:1765	rumen inoculum to total volume ratio	1730:1765	rumen inoculum to total volume ratio equal to 0.372	1730:1780	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	9	41	theme	significant	1582:1592	arg1	impact					1594:1599	a significant impact	1580:1599	a significant impact	1580:1599	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	7	42	theme	shorter	1210:1216	arg1	acids					1224:1228	shorter fatty acids	1210:1228	shorter fatty acids produced	1210:1237	The temperature increase resulted in shorter fatty acids produced.					
30847541	5	43	theme	rumen	978:982	arg1	5.1 g/L					1002:1008	5.1 g/L	1002:1008	5.1 g/L	1002:1008	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	5	43	theme	rumen	978:982	arg1	microbiome					984:993	the rumen microbiome	974:993	the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate	974:1061	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	6	44	theme	short	1139:1143	arg1	acids					1157:1161	the short chain fatty acids	1135:1161	the short chain fatty acids produced	1135:1170	Fermentation temperature was crucial especially for the composition of the short chain fatty acids produced.					
30847541	1	45	theme	multispecies	154:165	arg1	reactors					184:191	the multispecies biofilm membrane reactors	150:191	the multispecies biofilm membrane reactors (MBM reactors)	150:206	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	1	45	theme	multispecies	154:165	arg1	reactors					198:205	MBM reactors	194:205	MBM reactors	194:205	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	9	46	theme	inoculum	1736:1743	arg1	ratio					1761:1765	rumen inoculum to total volume ratio	1730:1765	rumen inoculum to total volume ratio equal to 0.372	1730:1780	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	9	47	theme	total	1608:1612	arg1	yield					1614:1618	the total yield	1604:1618	the total yield of organic acids produced	1604:1644	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	3	48	used	used	494:497	arg2	consortium					527:536	the converting microbial consortium	502:536	the converting microbial consortium	502:536	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	3	48	used	used	494:497	arg2	microbiome					479:488	The rumen microbiome	469:488	The rumen microbiome	469:488	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	1	49	theme	membrane	175:182	arg1	reactors					184:191	the multispecies biofilm membrane reactors	150:191	the multispecies biofilm membrane reactors (MBM reactors)	150:206	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	1	49	theme	membrane	175:182	arg1	reactors					198:205	MBM reactors	194:205	MBM reactors	194:205	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	0	50	theme	rumen	117:121	arg1	microbiome					123:132	the rumen microbiome	113:132	the rumen microbiome	113:132	A cellulolytic fungal biofilm enhances the consolidated bioconversion of cellulose to short chain fatty acids by the rumen microbiome.					
30847541	3	51	theme	microbial	517:525	arg1	consortium					527:536	the converting microbial consortium	502:536	the converting microbial consortium	502:536	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	3	51	theme	microbial	517:525	arg1	microbiome					479:488	The rumen microbiome	469:488	The rumen microbiome	469:488	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	9	52	with	pH 6	1719:1722	arg1	ratio					1761:1765	rumen inoculum to total volume ratio	1730:1765	rumen inoculum to total volume ratio equal to 0.372	1730:1780	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	9	53	theme	acids	1631:1635	arg1	yield					1614:1618	the total yield	1604:1618	the total yield of organic acids produced	1604:1644	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	8	54	theme	butyric	1278:1284	arg1	acids					1299:1303	acetic, propionic, butyric, and caproic acids	1259:1303	acetic, propionic, butyric, and caproic acids	1259:1303	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	9	55	dep	had	1576:1578	arg1	reached					1653:1659	reached	1653:1659	reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372)	1653:1781	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	9	56	contain	had	1576:1578	arg1	parameter					1558:1566	no parameter	1555:1566	no parameter studied	1555:1574	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	9	56	contain	had	1576:1578	arg2	impact					1594:1599	a significant impact	1580:1599	a significant impact	1580:1599	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	1	57	theme	short	373:377	arg1	SCFAs					398:402	SCFAs	398:402	SCFAs	398:402	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	1	57	theme	short	373:377	arg1	acids					391:395	short chain fatty acids	373:395	short chain fatty acids (SCFAs)	373:403	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	3	58	theme	individual	567:576	arg1	fungi					586:590	selected individual aerobic fungi	558:590	selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen	558:657	The rumen microbiome was used as the converting microbial consortium, co-cultivated with selected individual aerobic fungi which formed a biofilm on the tubular membrane flushed with oxygen.					
30847541	8	59	from	37.5 °C	1432:1438	arg1	fermentations					1415:1427	the fermentations	1411:1427	the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus	1411:1505	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	1	60	theme	fatty	385:389	arg1	SCFAs					398:402	SCFAs	398:402	SCFAs	398:402	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	1	60	theme	fatty	385:389	arg1	acids					391:395	short chain fatty acids	373:395	short chain fatty acids (SCFAs)	373:403	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	5	61	theme	Trichoderma	890:900	arg1	biofilm					909:915	Trichoderma reesei biofilm	890:915	Trichoderma reesei biofilm	890:915	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	9	62	with	30 °C	1706:1710	arg1	ratio					1761:1765	rumen inoculum to total volume ratio	1730:1765	rumen inoculum to total volume ratio equal to 0.372	1730:1780	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	8	63	theme	acetic	1259:1264	arg1	acids					1299:1303	acetic, propionic, butyric, and caproic acids	1259:1303	acetic, propionic, butyric, and caproic acids	1259:1303	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	0	64	theme	cellulose	73:81	arg1	bioconversion					56:68	the consolidated bioconversion	39:68	the consolidated bioconversion of cellulose to short chain fatty acids	39:108	A cellulolytic fungal biofilm enhances the consolidated bioconversion of cellulose to short chain fatty acids by the rumen microbiome.					
30847541	9	65	theme	SCFAs	1677:1681	arg1	0.47 g					1661:1666	0.47 g	1661:1666	0.47 g of total SCFAs per g of cellulose	1661:1700	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	6	66	theme	fatty	1151:1155	arg1	acids					1157:1161	the short chain fatty acids	1135:1161	the short chain fatty acids produced	1135:1170	Fermentation temperature was crucial especially for the composition of the short chain fatty acids produced.					
30847541	8	67	theme	propionic	1267:1275	arg1	acids					1299:1303	acetic, propionic, butyric, and caproic acids	1259:1303	acetic, propionic, butyric, and caproic acids	1259:1303	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	0	68	theme	short	86:90	arg1	acids					104:108	short chain fatty acids	86:108	short chain fatty acids	86:108	A cellulolytic fungal biofilm enhances the consolidated bioconversion of cellulose to short chain fatty acids by the rumen microbiome.					
30847541	8	69	theme	Coprinopsis	1457:1467	arg1	cinerea					1469:1475	Coprinopsis cinerea	1457:1475	Coprinopsis cinerea	1457:1475	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	4	70	theme	beneficial	664:673	arg1	effect					675:680	The beneficial effect	660:680	The beneficial effect of the fungal biofilm on the process yields and productivities	660:743	The beneficial effect of the fungal biofilm on the process yields and productivities was attributed to the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone.					
30847541	0	71	theme	fatty	98:102	arg1	acids					104:108	short chain fatty acids	86:108	short chain fatty acids	86:108	A cellulolytic fungal biofilm enhances the consolidated bioconversion of cellulose to short chain fatty acids by the rumen microbiome.					
30847541	9	72	dep	ratio	1761:1765	arg1	to					1745:1746	to	1745:1746	to	1745:1746	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	9	73	theme	cellulose	1692:1700	arg1	g					1687:1687	g	1687:1687	g of cellulose	1687:1700	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	1	74	theme	bioprocessing	343:355	arg1	investigation					309:321	the investigation	305:321	the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs)	305:403	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	5	75	theme	higher	945:950	arg1	concentration					927:939	a concentration	925:939	a concentration 39% higher (7.3 g/L SCFAs)	925:966	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	8	76	theme	acids	1299:1303	arg1	mixture					1248:1254	a mixture	1246:1254	a mixture of acetic, propionic, butyric, and caproic acids	1246:1303	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	0	77	theme	cellulolytic	2:13	arg1	biofilm					22:28	A cellulolytic fungal biofilm	0:28	A cellulolytic fungal biofilm	0:28	A cellulolytic fungal biofilm enhances the consolidated bioconversion of cellulose to short chain fatty acids by the rumen microbiome.					
30847541	1	78	theme	cellulose	360:368	arg1	bioprocessing					343:355	the consolidated bioprocessing	326:355	the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs)	326:403	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	2	79	theme	consortium	408:417	arg1	bioprocess					438:447	A consortium based consolidated bioprocess	406:447	A consortium based consolidated bioprocess (CBP)	406:453	A consortium based consolidated bioprocess (CBP) was designed.					
30847541	2	79	theme	consortium	408:417	arg1	CBP					450:452	CBP	450:452	CBP	450:452	A consortium based consolidated bioprocess (CBP) was designed.					
30847541	5	80	theme	15 g/L	1017:1022	arg1	substrate					1053:1061	the substrate	1049:1061	the substrate	1049:1061	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	5	80	theme	15 g/L	1017:1022	arg1	cellulose					1036:1044	15 g/L crystalline cellulose	1017:1044	15 g/L crystalline cellulose	1017:1044	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	1	81	theme	same	282:285	arg1	time					287:290	the same time	278:290	the same time	278:290	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	9	82	theme	biofilm	1546:1552	arg1	presence					1523:1530	the presence	1519:1530	the presence of the fungal biofilm	1519:1552	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	2	83	theme	consolidated	425:436	arg1	bioprocess					438:447	A consortium based consolidated bioprocess	406:447	A consortium based consolidated bioprocess (CBP)	406:453	A consortium based consolidated bioprocess (CBP) was designed.					
30847541	2	83	theme	consolidated	425:436	arg1	CBP					450:452	CBP	450:452	CBP	450:452	A consortium based consolidated bioprocess (CBP) was designed.					
30847541	1	84	theme	chain	379:383	arg1	SCFAs					398:402	SCFAs	398:402	SCFAs	398:402	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	1	84	theme	chain	379:383	arg1	acids					391:395	short chain fatty acids	373:395	short chain fatty acids (SCFAs)	373:403	The ability of the multispecies biofilm membrane reactors (MBM reactors) to provide distinguished niches for aerobic and anaerobic microbes at the same time was used for the investigation of the consolidated bioprocessing of cellulose to short chain fatty acids (SCFAs).					
30847541	4	85	theme	enhanced	767:774	arg1	activities					789:798	the enhanced cellulolytic activities	763:798	the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone	763:857	The beneficial effect of the fungal biofilm on the process yields and productivities was attributed to the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone.					
30847541	5	86	theme	7.3 g/L	953:959	arg1	SCFAs					961:965	7.3 g/L SCFAs	953:965	7.3 g/L SCFAs	953:965	At 30 °C, the MBM system with Trichoderma reesei biofilm reached a concentration 39% higher (7.3 g/L SCFAs), than the rumen microbiome alone (5.1 g/L) using 15 g/L crystalline cellulose as the substrate.					
30847541	9	87	theme	total	1748:1752	arg1	ratio					1761:1765	rumen inoculum to total volume ratio	1730:1765	rumen inoculum to total volume ratio equal to 0.372	1730:1780	Apart from the presence of the fungal biofilm, no parameter studied had a significant impact on the total yield of organic acids produced, which reached 0.47 g of total SCFAs per g of cellulose (at 30 °C and at pH 6, with rumen inoculum to total volume ratio equal to 0.372).					
30847541	8	88	theme	butyric	1360:1366	arg1	acids					1380:1384	butyric and caproic acids	1360:1384	butyric and caproic acids	1360:1384	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	7	89	theme	fatty	1218:1222	arg1	acids					1224:1228	shorter fatty acids	1210:1228	shorter fatty acids produced	1210:1237	The temperature increase resulted in shorter fatty acids produced.					
30847541	8	90	theme	caproic	1372:1378	arg1	acids					1380:1384	butyric and caproic acids	1360:1384	butyric and caproic acids	1360:1384	While a mixture of acetic, propionic, butyric, and caproic acids was produced at 30 °C with Trichoderma reesei biofilm, butyric and caproic acids were not detected during the fermentations at 37.5 °C carried out with Coprinopsis cinerea as the biofilm forming fungus.					
30847541	4	91	from	effect	675:680	arg1	process					711:717	the process yields and productivities	707:743	process	711:717	The beneficial effect of the fungal biofilm on the process yields and productivities was attributed to the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone.					
30847541	4	91	from	effect	675:680	arg1	productivities					730:743	productivities	730:743	productivities	730:743	The beneficial effect of the fungal biofilm on the process yields and productivities was attributed to the enhanced cellulolytic activities compared with those achieved by the rumen microbiome alone.					
29842888	6	0	theme	surface	743:749	arg1	charge					751:756	a surface charge	741:756	a surface charge of -47 mV	741:766	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	9	1	theme	daf-2	1182:1186	arg1	mutant					1188:1193	daf-2 mutant	1182:1193	daf-2 mutant	1182:1193	GB-loaded nanoparticles produced a hypolipidemic effect over C. elegans N2 wild-type and daf-2 mutant.					
29842888	2	2	theme	poly	199:202	arg1	nanoparticles					215:227	cyclodextrins-modified poly(anhydride) nanoparticles	176:227	cyclodextrins-modified poly(anhydride) nanoparticles to enhance the oral administration of glibenclamide	176:279	The aim of this work was to prepare and evaluate cyclodextrins-modified poly(anhydride) nanoparticles to enhance the oral administration of glibenclamide.					
29842888	8	3	theme	simulated	1065:1073	arg1	fluid					1086:1090	simulated intestinal fluid	1065:1090	simulated intestinal fluid	1065:1090	Glibenclamide was mainly release by Fickian-diffusion in simulated intestinal fluid.					
29842888	9	4	theme	GB-loaded	1093:1101	arg1	nanoparticles					1103:1115	GB-loaded nanoparticles	1093:1115	GB-loaded nanoparticles	1093:1115	GB-loaded nanoparticles produced a hypolipidemic effect over C. elegans N2 wild-type and daf-2 mutant.					
29842888	11	5	from	strategy	1419:1426	arg1	summary					1317:1323	summary	1317:1323	summary	1317:1323	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	3	6	theme	poly	383:386	arg1	backbone					371:378	the backbone	367:378	the backbone of poly(methylvinyl ether-co-maleic anhydride)	367:425	A conjugate polymer was synthesized by incorporating hydroxypropyl-β-cyclodextrin to the backbone of poly(methylvinyl ether-co-maleic anhydride) via Steglich reaction.					
29842888	8	7	theme	intestinal	1075:1084	arg1	fluid					1086:1090	simulated intestinal fluid	1065:1090	simulated intestinal fluid	1065:1090	Glibenclamide was mainly release by Fickian-diffusion in simulated intestinal fluid.					
29842888	7	8	dep	nanoparticles	914:926	arg1	entrapped					975:983	entrapped	975:983	entrapped in the polymer chains	975:1005	X-ray diffraction studies confirmed the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains.					
29842888	7	8	dep	nanoparticles	914:926	arg1	included					936:943	included	936:943	included into cyclodextrin cavities	936:970	X-ray diffraction studies confirmed the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains.					
29842888	9	9	dep	C.	1154:1155	arg1	elegans					1157:1163	elegans	1157:1163	elegans	1157:1163	GB-loaded nanoparticles produced a hypolipidemic effect over C. elegans N2 wild-type and daf-2 mutant.					
29842888	6	10	theme	drug	774:777	arg1	loading					779:785	a drug loading	772:785	a drug loading of 69 µg GB/mg	772:800	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	5	11	used	used	621:624	arg2	design					595:600	A central composite design	575:600	A central composite design of experiments	575:615	A central composite design of experiments was used to optimize the preparative process.					
29842888	4	12	theme	cyclodextrins	499:511	arg1	molecules					513:521	cyclodextrins molecules	499:521	cyclodextrins molecules	499:521	The degree of substitution of anhydride rings by cyclodextrins molecules was calculated to be 4.9% using H-NMR spectroscopy.					
29842888	5	13	theme	preparative	642:652	arg1	process					654:660	the preparative process	638:660	the preparative process	638:660	A central composite design of experiments was used to optimize the preparative process.					
29842888	11	14	theme	anhydride	1375:1383	arg1	backbone					1386:1393	the poly(anhydride) backbone	1366:1393	the poly(anhydride) backbone	1366:1393	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	7	15	theme	cyclodextrin	950:961	arg1	cavities					963:970	cyclodextrin cavities	950:970	cyclodextrin cavities	950:970	X-ray diffraction studies confirmed the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains.					
29842888	1	16	theme	In	90:91	arg1	evaluation					98:107	In vivo evaluation	90:107	In vivo evaluation	90:107	In vivo evaluation using C. elegans.					
29842888	6	17	theme	170 nm	733:738	arg1	size					719:722	a size	717:722	a size of about 170 nm	717:738	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	6	17	theme	170 nm	733:738	arg1	charge					751:756	a surface charge	741:756	a surface charge of -47 mV	741:766	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	6	17	theme	170 nm	733:738	arg1	loading					779:785	a drug loading	772:785	a drug loading of 69 µg GB/mg	772:800	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	5	18	theme	experiments	605:615	arg1	design					595:600	A central composite design	575:600	A central composite design of experiments	575:615	A central composite design of experiments was used to optimize the preparative process.					
29842888	2	19	theme	work	143:146	arg1	aim					131:133	The aim	127:133	The aim of this work	127:146	The aim of this work was to prepare and evaluate cyclodextrins-modified poly(anhydride) nanoparticles to enhance the oral administration of glibenclamide.					
29842888	0	20	theme	Cyclodextrin-grafted	0:19	arg1	anhydride					26:34	anhydride	26:34	anhydride	26:34	Cyclodextrin-grafted poly(anhydride) nanoparticles for oral glibenclamide administration.					
29842888	0	20	theme	Cyclodextrin-grafted	0:19	arg1	poly					21:24	Cyclodextrin-grafted poly	0:24	Cyclodextrin-grafted poly(anhydride)	0:35	Cyclodextrin-grafted poly(anhydride) nanoparticles for oral glibenclamide administration.					
29842888	9	21	theme	hypolipidemic	1128:1140	arg1	effect					1142:1147	a hypolipidemic effect	1126:1147	a hypolipidemic effect	1126:1147	GB-loaded nanoparticles produced a hypolipidemic effect over C. elegans N2 wild-type and daf-2 mutant.					
29842888	6	22	theme	69 µg GB/mg	790:800	arg1	size					719:722	a size	717:722	a size of about 170 nm	717:738	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	6	22	theme	69 µg GB/mg	790:800	arg1	charge					751:756	a surface charge	741:756	a surface charge of -47 mV	741:766	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	6	22	theme	69 µg GB/mg	790:800	arg1	loading					779:785	a drug loading	772:785	a drug loading of 69 µg GB/mg	772:800	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	11	23	theme	oral	1461:1464	arg1	administration					1466:1479	the oral administration	1457:1479	the oral administration of glibenclamide	1457:1496	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	3	24	theme	methylvinyl	388:398	arg1	anhydride					416:424	methylvinyl ether-co-maleic anhydride	388:424	methylvinyl ether-co-maleic anhydride	388:424	A conjugate polymer was synthesized by incorporating hydroxypropyl-β-cyclodextrin to the backbone of poly(methylvinyl ether-co-maleic anhydride) via Steglich reaction.					
29842888	3	24	theme	methylvinyl	388:398	arg1	poly					383:386	poly	383:386	poly(methylvinyl ether-co-maleic anhydride)	383:425	A conjugate polymer was synthesized by incorporating hydroxypropyl-β-cyclodextrin to the backbone of poly(methylvinyl ether-co-maleic anhydride) via Steglich reaction.					
29842888	7	25	theme	crystalline	855:865	arg1	structure					867:875	the crystalline structure	851:875	the crystalline structure of GB	851:881	X-ray diffraction studies confirmed the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains.					
29842888	3	26	theme	ether-co-maleic	400:414	arg1	anhydride					416:424	methylvinyl ether-co-maleic anhydride	388:424	methylvinyl ether-co-maleic anhydride	388:424	A conjugate polymer was synthesized by incorporating hydroxypropyl-β-cyclodextrin to the backbone of poly(methylvinyl ether-co-maleic anhydride) via Steglich reaction.					
29842888	3	26	theme	ether-co-maleic	400:414	arg1	poly					383:386	poly	383:386	poly(methylvinyl ether-co-maleic anhydride)	383:425	A conjugate polymer was synthesized by incorporating hydroxypropyl-β-cyclodextrin to the backbone of poly(methylvinyl ether-co-maleic anhydride) via Steglich reaction.					
29842888	7	27	theme	diffraction	809:819	arg1	studies					821:827	X-ray diffraction studies	803:827	X-ray diffraction studies	803:827	X-ray diffraction studies confirmed the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains.					
29842888	3	28	theme	conjugate	284:292	arg1	polymer					294:300	A conjugate polymer	282:300	A conjugate polymer	282:300	A conjugate polymer was synthesized by incorporating hydroxypropyl-β-cyclodextrin to the backbone of poly(methylvinyl ether-co-maleic anhydride) via Steglich reaction.					
29842888	4	29	theme	anhydride	480:488	arg1	rings					490:494	anhydride rings	480:494	anhydride rings	480:494	The degree of substitution of anhydride rings by cyclodextrins molecules was calculated to be 4.9% using H-NMR spectroscopy.					
29842888	11	30	theme	glibenclamide	1484:1496	arg1	administration					1466:1479	the oral administration	1457:1479	the oral administration of glibenclamide	1457:1496	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	9	31	theme	N2	1165:1166	arg1	wild-type					1168:1176	C. elegans N2 wild-type	1154:1176	C. elegans N2 wild-type	1154:1176	GB-loaded nanoparticles produced a hypolipidemic effect over C. elegans N2 wild-type and daf-2 mutant.					
29842888	4	32	theme	H-NMR	555:559	arg1	spectroscopy					561:572	H-NMR spectroscopy	555:572	H-NMR spectroscopy	555:572	The degree of substitution of anhydride rings by cyclodextrins molecules was calculated to be 4.9% using H-NMR spectroscopy.					
29842888	11	33	theme	cyclodextrin	1350:1361	arg1	linkage					1339:1345	the covalent linkage	1326:1345	the covalent linkage of cyclodextrin to the poly(anhydride) backbone	1326:1393	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	11	33	theme	cyclodextrin	1350:1361	arg1	strategy					1419:1426	an interesting strategy	1404:1426	an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide	1404:1496	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	7	34	theme	GB	880:881	arg1	structure					867:875	the crystalline structure	851:875	the crystalline structure of GB	851:881	X-ray diffraction studies confirmed the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains.					
29842888	0	35	theme	oral	55:58	arg1	administration					74:87	oral glibenclamide administration	55:87	oral glibenclamide administration	55:87	Cyclodextrin-grafted poly(anhydride) nanoparticles for oral glibenclamide administration.					
29842888	7	36	theme	X-ray	803:807	arg1	studies					821:827	X-ray diffraction studies	803:827	X-ray diffraction studies	803:827	X-ray diffraction studies confirmed the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains.					
29842888	4	37	theme	rings	490:494	arg1	substitution					464:475	substitution	464:475	substitution of anhydride rings by cyclodextrins molecules	464:521	The degree of substitution of anhydride rings by cyclodextrins molecules was calculated to be 4.9% using H-NMR spectroscopy.					
29842888	5	38	theme	central	577:583	arg1	design					595:600	A central composite design	575:600	A central composite design of experiments	575:615	A central composite design of experiments was used to optimize the preparative process.					
29842888	3	39	theme	Steglich	431:438	arg1	reaction					440:447	Steglich reaction	431:447	Steglich reaction	431:447	A conjugate polymer was synthesized by incorporating hydroxypropyl-β-cyclodextrin to the backbone of poly(methylvinyl ether-co-maleic anhydride) via Steglich reaction.					
29842888	11	40	theme	poly	1370:1373	arg1	backbone					1386:1393	the poly(anhydride) backbone	1366:1393	the poly(anhydride) backbone	1366:1393	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	5	41	theme	composite	585:593	arg1	design					595:600	A central composite design	575:600	A central composite design of experiments	575:615	A central composite design of experiments was used to optimize the preparative process.					
29842888	1	42	dep	In	90:91	arg1	vivo					93:96	vivo	93:96	vivo	93:96	In vivo evaluation using C. elegans.					
29842888	10	43	theme	signaling	1281:1289	arg1	pathway					1291:1297	the insulin signaling pathway	1269:1297	the insulin signaling pathway of C. elegans	1269:1311	The action mechanism included daf-2 and daf-28 genes, both implicated in the insulin signaling pathway of C. elegans.					
29842888	10	44	dep	included	1217:1224	arg1	both					1250:1253	both	1250:1253	both	1250:1253	The action mechanism included daf-2 and daf-28 genes, both implicated in the insulin signaling pathway of C. elegans.					
29842888	10	45	theme	insulin	1273:1279	arg1	pathway					1291:1297	the insulin signaling pathway	1269:1297	the insulin signaling pathway of C. elegans	1269:1311	The action mechanism included daf-2 and daf-28 genes, both implicated in the insulin signaling pathway of C. elegans.					
29842888	10	46	theme	daf-28	1236:1241	arg1	genes					1243:1247	daf-2 and daf-28 genes	1226:1247	daf-2 and daf-28 genes	1226:1247	The action mechanism included daf-2 and daf-28 genes, both implicated in the insulin signaling pathway of C. elegans.					
29842888	2	47	theme	glibenclamide	267:279	arg1	administration					249:262	the oral administration	240:262	the oral administration of glibenclamide	240:279	The aim of this work was to prepare and evaluate cyclodextrins-modified poly(anhydride) nanoparticles to enhance the oral administration of glibenclamide.					
29842888	4	48	theme	substitution	464:475	arg1	degree					454:459	The degree	450:459	The degree of substitution of anhydride rings by cyclodextrins molecules	450:521	The degree of substitution of anhydride rings by cyclodextrins molecules was calculated to be 4.9% using H-NMR spectroscopy.					
29842888	4	48	theme	substitution	464:475	arg1	%					547:547	4.9%	544:547	4.9%	544:547	The degree of substitution of anhydride rings by cyclodextrins molecules was calculated to be 4.9% using H-NMR spectroscopy.					
29842888	11	49	theme	interesting	1407:1417	arg1	linkage					1339:1345	the covalent linkage	1326:1345	the covalent linkage of cyclodextrin to the poly(anhydride) backbone	1326:1393	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	11	49	theme	interesting	1407:1417	arg1	strategy					1419:1426	an interesting strategy	1404:1426	an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide	1404:1496	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	6	50	theme	-47 mV	761:766	arg1	size					719:722	a size	717:722	a size of about 170 nm	717:738	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	6	50	theme	-47 mV	761:766	arg1	charge					751:756	a surface charge	741:756	a surface charge of -47 mV	741:766	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	6	50	theme	-47 mV	761:766	arg1	loading					779:785	a drug loading	772:785	a drug loading of 69 µg GB/mg	772:800	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	9	51	theme	C.	1154:1155	arg1	wild-type					1168:1176	C. elegans N2 wild-type	1154:1176	C. elegans N2 wild-type	1154:1176	GB-loaded nanoparticles produced a hypolipidemic effect over C. elegans N2 wild-type and daf-2 mutant.					
29842888	7	52	theme	structure	867:875	arg1	loss					843:846	the loss	839:846	the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains	839:1005	X-ray diffraction studies confirmed the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains.					
29842888	2	53	theme	cyclodextrins-modified	176:197	arg1	poly					199:202	cyclodextrins-modified poly	176:202	cyclodextrins-modified poly(anhydride) nanoparticles to enhance the oral administration of glibenclamide	176:279	The aim of this work was to prepare and evaluate cyclodextrins-modified poly(anhydride) nanoparticles to enhance the oral administration of glibenclamide.					
29842888	2	53	theme	cyclodextrins-modified	176:197	arg1	anhydride					204:212	anhydride	204:212	anhydride	204:212	The aim of this work was to prepare and evaluate cyclodextrins-modified poly(anhydride) nanoparticles to enhance the oral administration of glibenclamide.					
29842888	2	54	theme	oral	244:247	arg1	administration					249:262	the oral administration	240:262	the oral administration of glibenclamide	240:279	The aim of this work was to prepare and evaluate cyclodextrins-modified poly(anhydride) nanoparticles to enhance the oral administration of glibenclamide.					
29842888	6	55	theme	optimal	673:679	arg1	conditions					681:690	the optimal conditions	669:690	the optimal conditions	669:690	Under the optimal conditions, nanoparticles displayed a size of about 170 nm, a surface charge of -47 mV and a drug loading of 69 µg GB/mg.					
29842888	10	56	theme	daf-2	1226:1230	arg1	genes					1243:1247	daf-2 and daf-28 genes	1226:1247	daf-2 and daf-28 genes	1226:1247	The action mechanism included daf-2 and daf-28 genes, both implicated in the insulin signaling pathway of C. elegans.					
29842888	0	57	theme	glibenclamide	60:72	arg1	administration					74:87	oral glibenclamide administration	55:87	oral glibenclamide administration	55:87	Cyclodextrin-grafted poly(anhydride) nanoparticles for oral glibenclamide administration.					
29842888	11	58	theme	covalent	1330:1337	arg1	linkage					1339:1345	the covalent linkage	1326:1345	the covalent linkage of cyclodextrin to the poly(anhydride) backbone	1326:1393	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	11	58	theme	covalent	1330:1337	arg1	strategy					1419:1426	an interesting strategy	1404:1426	an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide	1404:1496	In summary, the covalent linkage of cyclodextrin to the poly(anhydride) backbone could be an interesting strategy to prepare nanoparticles for the oral administration of glibenclamide.					
29842888	7	59	theme	polymer	992:998	arg1	chains					1000:1005	the polymer chains	988:1005	the polymer chains	988:1005	X-ray diffraction studies confirmed the loss of the crystalline structure of GB due to its dispersion into the nanoparticles, either included into cyclodextrin cavities or entrapped in the polymer chains.					
29842888	8	60	from	release	1033:1039	arg1	fluid					1086:1090	simulated intestinal fluid	1065:1090	simulated intestinal fluid	1065:1090	Glibenclamide was mainly release by Fickian-diffusion in simulated intestinal fluid.					
29842888	10	61	theme	elegans	1305:1311	arg1	pathway					1291:1297	the insulin signaling pathway	1269:1297	the insulin signaling pathway of C. elegans	1269:1311	The action mechanism included daf-2 and daf-28 genes, both implicated in the insulin signaling pathway of C. elegans.					
29842888	10	62	theme	action	1200:1205	arg1	mechanism					1207:1215	The action mechanism	1196:1215	The action mechanism	1196:1215	The action mechanism included daf-2 and daf-28 genes, both implicated in the insulin signaling pathway of C. elegans.					
31262003	4	0	theme	beneficial	924:933	arg1	bacteria					935:942	beneficial bacteria	924:942	beneficial bacteria	924:942	The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin.					
31262003	5	1	theme	fiber	1145:1149	arg1	fermentation					1102:1113	the fermentation	1098:1113	the fermentation of partly-fermentable dietary fiber	1098:1149	Accordingly, PSP might be a material conducive for improving the conditions for the fermentation of partly-fermentable dietary fiber.					
31262003	2	2	dep	in	456:457	arg1	vitro					459:463	vitro	459:463	vitro	459:463	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	3	3	dep	used	532:535	arg1	cellulose					716:724	cellulose	716:724	cellulose	716:724	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	3	3	dep	used	532:535	arg1	Cellulose					705:713	Cellulose	705:713	Cellulose	705:713	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	3	3	dep	used	532:535	arg1	inulin					733:738	inulin	733:738	inulin	733:738	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	3	3	dep	used	532:535	arg1	inulin					745:750	inulin	745:750	inulin	745:750	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	4	4	theme	purple	819:824	arg1	potato					832:837	purple sweet potato	819:837	purple sweet potato	819:837	The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin.					
31262003	1	5	theme	poor	248:251	arg1	nutrition					253:261	poor nutrition	248:261	poor nutrition	248:261	The prevalence of many chronic diseases which have been associated with poor nutrition may be reduced by the positive modulation of colonic microbiota.					
31262003	2	6	theme	bacteria	440:447	arg1	culture					417:423	a mixed culture	409:423	a mixed culture of swine fecal bacteria	409:447	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	3	7	theme	Jar	644:646	arg1	fermenters					648:657	Jar fermenters	644:657	Jar fermenters	644:657	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	6	8	theme	products	1228:1235	arg1	reduction					1202:1210	the drastic reduction	1190:1210	the drastic reduction of putrefactive products, especially p-cresol to a significant level	1190:1279	Besides, PSP was also responsible for the drastic reduction of putrefactive products, especially p-cresol to a significant level.					
31262003	2	9	theme	colonic	465:471	arg1	fermentation					473:484	in vitro colonic fermentation	456:484	in vitro colonic fermentation using pig colonic digest	456:509	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	2	10	theme	pig	492:494	arg1	digest					504:509	pig colonic digest	492:509	pig colonic digest	492:509	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	7	11	theme	stable	1436:1441	arg1	environment					1463:1473	a stable and healthy colonic environment	1434:1473	a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host	1434:1572	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	4	12	theme	potato	832:837	arg1	content					808:814	the polyphenolic content	791:814	the polyphenolic content of purple sweet potato	791:837	The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin.					
31262003	4	13	theme	polyphenolic	795:806	arg1	content					808:814	the polyphenolic content	791:814	the polyphenolic content of purple sweet potato	791:837	The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin.					
31262003	3	14	theme	Jar	512:514	arg1	fermenters					516:525	Jar fermenters	512:525	Jar fermenters	512:525	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	4	15	theme	sweet	826:830	arg1	potato					832:837	purple sweet potato	819:837	purple sweet potato	819:837	The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin.					
31262003	3	16	dep	h.	641:642	arg1	assigned					664:671	assigned	664:671	were assigned to one of the following groups	659:702	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	0	17	theme	Fiber	126:130	arg1	Fermentability					100:113	the Fermentability	96:113	the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria	96:173	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	4	18	theme	present	763:769	arg1	study					771:775	The present study	759:775	The present study	759:775	The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin.					
31262003	7	19	theme	dietary	1386:1392	arg1	fiber					1394:1398	dietary fiber	1386:1398	dietary fiber	1386:1398	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	2	20	theme	sweet	375:379	arg1	polyphenols					388:398	purple sweet potato polyphenols	368:398	purple sweet potato polyphenols (PSP)	368:404	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	2	20	theme	sweet	375:379	arg1	PSP					401:403	PSP	401:403	PSP	401:403	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	0	21	theme	Dietary	118:124	arg1	Fiber					126:130	Dietary Fiber	118:130	Dietary Fiber	118:130	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	4	22	theme	pathogenic	963:972	arg1	bacteria					974:981	the pathogenic bacteria	959:981	the pathogenic bacteria depending on cellulose and inulin	959:1015	The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin.					
31262003	2	23	theme	purple	368:373	arg1	polyphenols					388:398	purple sweet potato polyphenols	368:398	purple sweet potato polyphenols (PSP)	368:404	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	2	23	theme	purple	368:373	arg1	PSP					401:403	PSP	401:403	PSP	401:403	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	0	24	theme	Sweet	7:11	arg1	Polyphenols					20:30	Purple Sweet Potato Polyphenols	0:30	Purple Sweet Potato Polyphenols	0:30	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	3	25	theme	groups	697:702	arg1	one					676:678	one	676:678	one	676:678	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	3	25	theme	groups	697:702	arg1	groups					697:702	the following groups	683:702	the following groups	683:702	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	7	26	theme	fiber	1394:1398	arg1	fermentability					1368:1381	the fermentability	1364:1381	the fermentability of dietary fiber	1364:1398	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	6	27	theme	significant	1263:1273	arg1	level					1275:1279	a significant level	1261:1279	a significant level	1261:1279	Besides, PSP was also responsible for the drastic reduction of putrefactive products, especially p-cresol to a significant level.					
31262003	0	28	theme	Purple	0:5	arg1	Polyphenols					20:30	Purple Sweet Potato Polyphenols	0:30	Purple Sweet Potato Polyphenols	0:30	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	5	29	theme	partly-fermentable	1118:1135	arg1	fiber					1145:1149	partly-fermentable dietary fiber	1118:1149	partly-fermentable dietary fiber	1118:1149	Accordingly, PSP might be a material conducive for improving the conditions for the fermentation of partly-fermentable dietary fiber.					
31262003	6	30	theme	drastic	1194:1200	arg1	reduction					1202:1210	the drastic reduction	1190:1210	the drastic reduction of putrefactive products, especially p-cresol to a significant level	1190:1279	Besides, PSP was also responsible for the drastic reduction of putrefactive products, especially p-cresol to a significant level.					
31262003	7	31	theme	healthy	1447:1453	arg1	environment					1463:1473	a stable and healthy colonic environment	1434:1473	a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host	1434:1572	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	6	32	theme	putrefactive	1215:1226	arg1	products					1228:1235	putrefactive products	1215:1235	putrefactive products	1215:1235	Besides, PSP was also responsible for the drastic reduction of putrefactive products, especially p-cresol to a significant level.					
31262003	6	32	theme	putrefactive	1215:1226	arg1	p-cresol					1249:1256	p-cresol	1249:1256	p-cresol	1249:1256	Besides, PSP was also responsible for the drastic reduction of putrefactive products, especially p-cresol to a significant level.					
31262003	3	33	theme	anaerobic	614:622	arg1	condition					624:632	the anaerobic condition	610:632	the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups	610:702	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	5	34	theme	dietary	1137:1143	arg1	fiber					1145:1149	partly-fermentable dietary fiber	1118:1149	partly-fermentable dietary fiber	1118:1149	Accordingly, PSP might be a material conducive for improving the conditions for the fermentation of partly-fermentable dietary fiber.					
31262003	1	35	theme	many	194:197	arg1	diseases					207:214	many chronic diseases	194:214	many chronic diseases which have been associated with poor nutrition	194:261	The prevalence of many chronic diseases which have been associated with poor nutrition may be reduced by the positive modulation of colonic microbiota.					
31262003	0	36	theme	Potato	13:18	arg1	Polyphenols					20:30	Purple Sweet Potato Polyphenols	0:30	Purple Sweet Potato Polyphenols	0:30	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	3	37	theme	following	687:695	arg1	groups					697:702	the following groups	683:702	the following groups	683:702	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	7	38	theme	health	1546:1551	arg1	benefits					1553:1560	health benefits	1546:1560	health benefits	1546:1560	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	7	39	theme	chronic	1506:1512	arg1	diseases					1514:1521	chronic diseases	1506:1521	chronic diseases development	1506:1533	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	1	40	theme	chronic	199:205	arg1	diseases					207:214	many chronic diseases	194:214	many chronic diseases which have been associated with poor nutrition	194:261	The prevalence of many chronic diseases which have been associated with poor nutrition may be reduced by the positive modulation of colonic microbiota.					
31262003	2	41	theme	fecal	434:438	arg1	bacteria					440:447	swine fecal bacteria	428:447	swine fecal bacteria	428:447	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	1	42	theme	positive	285:292	arg1	modulation					294:303	the positive modulation	281:303	the positive modulation of colonic microbiota	281:325	The prevalence of many chronic diseases which have been associated with poor nutrition may be reduced by the positive modulation of colonic microbiota.					
31262003	4	43	theme	colonic	858:864	arg1	microbiota					866:875	the colonic microbiota	854:875	the colonic microbiota	854:875	The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin.					
31262003	1	44	theme	diseases	207:214	arg1	prevalence					180:189	The prevalence	176:189	The prevalence of many chronic diseases which have been associated with poor nutrition	176:261	The prevalence of many chronic diseases which have been associated with poor nutrition may be reduced by the positive modulation of colonic microbiota.					
31262003	3	45	theme	small	550:554	arg1	scale					556:560	a small scale	548:560	a small scale	548:560	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	2	46	theme	polyphenols	388:398	arg1	effects					357:363	the effects	353:363	the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria	353:447	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	2	47	theme	swine	428:432	arg1	bacteria					440:447	swine fecal bacteria	428:447	swine fecal bacteria	428:447	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	0	48	theme	Mixed	137:141	arg1	Culture					143:149	a Mixed Culture	135:149	a Mixed Culture of Swine Fecal Bacteria	135:173	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	2	49	theme	potato	381:386	arg1	polyphenols					388:398	purple sweet potato polyphenols	368:398	purple sweet potato polyphenols (PSP)	368:404	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	2	49	theme	potato	381:386	arg1	PSP					401:403	PSP	401:403	PSP	401:403	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	0	50	theme	Microbial	61:69	arg1	Composition					71:81	the Microbial Composition	57:81	the Microbial Composition	57:81	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	0	51	theme	Swine	154:158	arg1	Bacteria					166:173	Swine Fecal Bacteria	154:173	Swine Fecal Bacteria	154:173	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	2	52	from	effects	357:363	arg1	culture					417:423	a mixed culture	409:423	a mixed culture of swine fecal bacteria	409:447	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	2	53	theme	colonic	496:502	arg1	digest					504:509	pig colonic digest	492:509	pig colonic digest	492:509	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	1	54	theme	colonic	308:314	arg1	microbiota					316:325	colonic microbiota	308:325	colonic microbiota	308:325	The prevalence of many chronic diseases which have been associated with poor nutrition may be reduced by the positive modulation of colonic microbiota.					
31262003	5	55	theme	conducive	1055:1063	arg1	material					1046:1053	a material	1044:1053	a material conducive for improving the conditions for the fermentation of partly-fermentable dietary fiber	1044:1149	Accordingly, PSP might be a material conducive for improving the conditions for the fermentation of partly-fermentable dietary fiber.					
31262003	5	55	theme	conducive	1055:1063	arg1	PSP					1031:1033	PSP	1031:1033	PSP	1031:1033	Accordingly, PSP might be a material conducive for improving the conditions for the fermentation of partly-fermentable dietary fiber.					
31262003	3	56	used	used	532:535	arg2	fermenters					516:525	Jar fermenters	512:525	Jar fermenters	512:525	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	7	57	theme	microbial	1327:1335	arg1	composition					1337:1347	the microbial composition	1323:1347	the microbial composition	1323:1347	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	7	58	theme	diseases	1514:1521	arg1	development					1523:1533	chronic diseases development	1506:1533	chronic diseases development	1506:1533	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	1	59	theme	microbiota	316:325	arg1	modulation					294:303	the positive modulation	281:303	the positive modulation of colonic microbiota	281:325	The prevalence of many chronic diseases which have been associated with poor nutrition may be reduced by the positive modulation of colonic microbiota.					
31262003	0	60	theme	Bacteria	166:173	arg1	Culture					143:149	a Mixed Culture	135:149	a Mixed Culture of Swine Fecal Bacteria	135:173	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	3	61	theme	colonic	571:577	arg1	experiments					592:602	vitro colonic fermentation experiments	565:602	vitro colonic fermentation experiments	565:602	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	4	62	theme	bacteria	935:942	arg1	population					910:919	the population	906:919	the population of beneficial bacteria	906:942	The present study revealed that the polyphenolic content of purple sweet potato could modulate the colonic microbiota by differentially increasing the population of beneficial bacteria and decreasing the pathogenic bacteria depending on cellulose and inulin.					
31262003	0	63	theme	Fecal	160:164	arg1	Bacteria					166:173	Swine Fecal Bacteria	154:173	Swine Fecal Bacteria	154:173	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	7	64	theme	colonic	1455:1461	arg1	environment					1463:1473	a stable and healthy colonic environment	1434:1473	a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host	1434:1572	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	3	65	theme	fermentation	579:590	arg1	experiments					592:602	vitro colonic fermentation experiments	565:602	vitro colonic fermentation experiments	565:602	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	0	66	from	Fermentability	100:113	arg1	Culture					143:149	a Mixed Culture	135:149	a Mixed Culture of Swine Fecal Bacteria	135:173	Purple Sweet Potato Polyphenols Differentially Influence the Microbial Composition Depending on the Fermentability of Dietary Fiber in a Mixed Culture of Swine Fecal Bacteria.					
31262003	7	67	contain	has	1404:1406	arg1	PSP					1307:1309	PSP	1307:1309	PSP	1307:1309	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	7	67	contain	has	1404:1406	arg2	potential					1412:1420	the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host	1408:1572	the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host	1408:1572	Our results suggest that PSP could alter the microbial composition depending upon the fermentability of dietary fiber and has the potential to maintain a stable and healthy colonic environment that will ultimately alleviate chronic diseases development and confer health benefits to the host.					
31262003	2	68	theme	in	456:457	arg1	fermentation					473:484	in vitro colonic fermentation	456:484	in vitro colonic fermentation using pig colonic digest	456:509	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31262003	3	69	theme	vitro	565:569	arg1	experiments					592:602	vitro colonic fermentation experiments	565:602	vitro colonic fermentation experiments	565:602	Jar fermenters were used to conduct a small scale in vitro colonic fermentation experiments under the anaerobic condition for 48 h. Jar fermenters were assigned to one of the following groups: Cellulose, cellulose + PSP, inulin, and inulin + PSP.					
31262003	2	70	theme	mixed	411:415	arg1	culture					417:423	a mixed culture	409:423	a mixed culture of swine fecal bacteria	409:447	In this study, we assess the effects of purple sweet potato polyphenols (PSP) in a mixed culture of swine fecal bacteria during in vitro colonic fermentation using pig colonic digest.					
31015554	0	0	theme	vannamei	74:81	arg1	immunity					50:57	immunity	50:57	immunity	50:57	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	0	0	theme	vannamei	74:81	arg1	intestine					15:23	the intestine microbial	11:33	the intestine microbial	11:33	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	0	0	theme	vannamei	74:81	arg1	digestion					36:44	digestion	36:44	digestion	36:44	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	6	1	theme	immune	808:813	arg1	NO					846:847	NO	846:847	NO	846:847	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	1	theme	immune	808:813	arg1	PO					824:825	PO	824:825	PO	824:825	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	1	theme	immune	808:813	arg1	enzymes					815:821	immune enzymes	808:821	immune enzymes (PO, T-AOC, T-NOS, and NO)	808:848	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	1	theme	immune	808:813	arg1	T-NOS					835:839	T-NOS	835:839	T-NOS	835:839	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	1	2	theme	intestine	215:223	arg1	function					239:246	the intestine physiological function	211:246	the intestine physiological function of animals	211:257	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	3	3	from	indices	448:454	arg1	composition					402:412	the microbial composition	388:412	the microbial composition	388:412	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	3	3	from	indices	448:454	arg1	intestine					463:471	the intestine	459:471	the intestine of Litopenaeus vannamei	459:495	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	1	4	theme	physiological	225:237	arg1	function					239:246	the intestine physiological function	211:246	the intestine physiological function of animals	211:257	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	9	5	theme	beneficial	1420:1429	arg1	metabolites					1431:1441	beneficial metabolites	1420:1441	beneficial metabolites	1420:1441	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	0	6	from	Changes	0:6	arg1	immunity					50:57	immunity	50:57	immunity	50:57	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	0	6	from	Changes	0:6	arg1	intestine					15:23	the intestine microbial	11:33	the intestine microbial	11:33	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	0	6	from	Changes	0:6	arg1	digestion					36:44	digestion	36:44	digestion	36:44	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	10	7	theme	vannamei	1622:1629	arg1	health					1609:1614	the intestine health	1595:1614	the intestine health of L. vannamei	1595:1629	Our results revealed that dietary RS could improve the intestine health of L. vannamei, probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.					
31015554	3	8	theme	vannamei	488:495	arg1	intestine					463:471	the intestine	459:471	the intestine of Litopenaeus vannamei	459:495	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	4	9	theme	different	540:548	arg1	levels					550:555	different levels	540:555	different levels of RS	540:561	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	7	10	theme	microbial	1123:1131	arg1	intestine					1113:1121	the intestine microbial	1109:1131	the intestine microbial	1109:1131	A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial.					
31015554	6	11	theme	digestive	760:768	arg1	Tryp					789:792	Tryp	789:792	Tryp	789:792	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	11	theme	digestive	760:768	arg1	Pep					799:801	Pep	799:801	Pep	799:801	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	11	theme	digestive	760:768	arg1	LPS					784:786	LPS	784:786	LPS	784:786	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	11	theme	digestive	760:768	arg1	AMS					779:781	AMS	779:781	AMS	779:781	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	11	theme	digestive	760:768	arg1	enzymes					770:776	digestive enzymes	760:776	digestive enzymes (AMS, LPS, Tryp, and Pep)	760:802	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	9	12	theme	beneficial	1264:1273	arg1	bacteria					1275:1282	the beneficial bacteria	1260:1282	the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella)	1260:1345	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	12	theme	beneficial	1264:1273	arg1	Mesoflavibacter					1314:1328	Mesoflavibacter	1314:1328	Mesoflavibacter	1314:1328	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	12	theme	beneficial	1264:1273	arg1	Ruegeria					1296:1303	Ruegeria	1296:1303	Ruegeria	1296:1303	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	12	theme	beneficial	1264:1273	arg1	Mameliella					1335:1344	Mameliella	1335:1344	Mameliella	1335:1344	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	3	13	theme	digestive	419:427	arg1	indices					448:454	digestive and immune-related indices	419:454	digestive and immune-related indices in the intestine of Litopenaeus vannamei	419:495	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	0	14	theme	dietary	98:104	arg1	starch					116:121	dietary resistant starch	98:121	dietary resistant starch	98:121	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	0	15	from	digestion	36:44	arg1	response					86:93	response	86:93	response to dietary resistant starch	86:121	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	6	16	theme	expression	859:868	arg1	levels					870:875	the expression levels	855:875	the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19)	855:972	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	8	17	theme	Proteobacteria	1179:1192	arg1	abundances					1165:1174	the abundances	1161:1174	the abundances of Proteobacteria	1161:1192	Specifically, RS increased the abundances of Proteobacteria and decreased the abundance of Bacteroidetes.					
31015554	3	18	theme	RS	382:383	arg1	indices					448:454	digestive and immune-related indices	419:454	digestive and immune-related indices in the intestine of Litopenaeus vannamei	419:495	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	3	18	theme	RS	382:383	arg1	effects					363:369	the effects	359:369	the the effects of dietary RS on the microbial composition	355:412	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	5	19	theme	dietary	666:672	arg1	RS					674:675	dietary RS	666:675	dietary RS	666:675	The results showed that dietary RS improved the morphology of the intestine mucosa.					
31015554	10	20	theme	dietary	1570:1576	arg1	RS					1578:1579	dietary RS	1570:1579	dietary RS	1570:1579	Our results revealed that dietary RS could improve the intestine health of L. vannamei, probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.					
31015554	9	21	dep	RS	1540:1541	arg1	response					1520:1527	response	1520:1527	response	1520:1527	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	2	22	theme	RS	282:283	arg1	roles					273:277	the roles	269:277	the roles of RS on shrimp intestine health	269:310	However, the roles of RS on shrimp intestine health is unknown.					
31015554	2	22	theme	RS	282:283	arg1	unknown					315:321	unknown	315:321	unknown	315:321	However, the roles of RS on shrimp intestine health is unknown.					
31015554	7	23	theme	short-chain	1037:1047	arg1	SCFAs					1062:1066	SCFAs	1062:1066	SCFAs	1062:1066	A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial.					
31015554	7	23	theme	short-chain	1037:1047	arg1	acids					1055:1059	short-chain fatty acids	1037:1059	the short-chain fatty acids (SCFAs) contents	1033:1076	A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial.					
31015554	1	24	theme	animals	251:257	arg1	function					239:246	the intestine physiological function	211:246	the intestine physiological function of animals	211:257	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	10	25	theme	intestine	1599:1607	arg1	health					1609:1614	the intestine health	1595:1614	the intestine health of L. vannamei	1595:1629	Our results revealed that dietary RS could improve the intestine health of L. vannamei, probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.					
31015554	3	26	theme	dietary	374:380	arg1	RS					382:383	dietary RS	374:383	dietary RS	374:383	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	10	27	theme	microbial	1670:1678	arg1	composition					1680:1690	the intestine microbial composition	1656:1690	the intestine microbial composition	1656:1690	Our results revealed that dietary RS could improve the intestine health of L. vannamei, probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.					
31015554	8	28	theme	Bacteroidetes	1225:1237	arg1	abundance					1212:1220	the abundance	1208:1220	the abundance of Bacteroidetes	1208:1237	Specifically, RS increased the abundances of Proteobacteria and decreased the abundance of Bacteroidetes.					
31015554	9	29	theme	dietary	1532:1538	arg1	RS					1540:1541	dietary RS	1532:1541	dietary RS	1532:1541	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	10	30	theme	intestine	1660:1668	arg1	composition					1680:1690	the intestine microbial composition	1656:1690	the intestine microbial composition	1656:1690	Our results revealed that dietary RS could improve the intestine health of L. vannamei, probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.					
31015554	0	31	theme	resistant	106:114	arg1	starch					116:121	dietary resistant starch	98:121	dietary resistant starch	98:121	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	7	32	theme	intestine	1113:1121	arg1	composition					1094:1104	the composition	1090:1104	the composition of the intestine microbial	1090:1131	A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial.					
31015554	1	33	theme	fibre	174:178	arg1	constituent					151:161	a constituent	149:161	a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals	149:257	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	1	33	theme	fibre	174:178	arg1	starch					134:139	Resistant starch	124:139	Resistant starch (RS)	124:144	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	3	34	theme	immune-related	433:446	arg1	indices					448:454	digestive and immune-related indices	419:454	digestive and immune-related indices in the intestine of Litopenaeus vannamei	419:495	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	9	35	dep	pathogens	1460:1468	arg1	Formosa					1471:1477	Formosa	1471:1477	Formosa	1471:1477	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	35	dep	pathogens	1460:1468	arg1	pathogens					1460:1468	potential pathogens	1450:1468	potential pathogens (Formosa and Pseudoalteromonas)	1450:1500	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	35	dep	pathogens	1460:1468	arg1	Pseudoalteromonas					1483:1499	Pseudoalteromonas	1483:1499	Pseudoalteromonas	1483:1499	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	7	36	theme	dietary	1012:1018	arg1	RS					1020:1021	dietary RS	1012:1021	dietary RS	1012:1021	A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial.					
31015554	4	37	contain	containing	529:538	arg1	0 g/kg					564:569	0 g/kg	564:569	0 g/kg (Control)	564:579	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	37	contain	containing	529:538	arg1	50 g/kg					615:621	50 g/kg	615:621	50 g/kg (RS3)	615:627	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	37	contain	containing	529:538	arg2	levels					550:555	different levels	540:555	different levels of RS	540:561	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	37	contain	containing	529:538	arg1	30 g/kg					597:603	30 g/kg	597:603	30 g/kg (RS2)	597:609	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	37	contain	containing	529:538	arg1	10 g/kg					582:588	10 g/kg	582:588	10 g/kg (RS1)	582:594	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	37	contain	containing	529:538	arg1	diets					523:527	diets	523:527	diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3)	523:627	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	7	38	theme	fatty	1049:1053	arg1	SCFAs					1062:1066	SCFAs	1062:1066	SCFAs	1062:1066	A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial.					
31015554	7	38	theme	fatty	1049:1053	arg1	acids					1055:1059	short-chain fatty acids	1037:1059	the short-chain fatty acids (SCFAs) contents	1033:1076	A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial.					
31015554	5	39	theme	intestine	708:716	arg1	mucosa					718:723	the intestine mucosa	704:723	the intestine mucosa	704:723	The results showed that dietary RS improved the morphology of the intestine mucosa.					
31015554	0	40	theme	microbial	25:33	arg1	intestine					15:23	the intestine microbial	11:33	the intestine microbial	11:33	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	6	41	dep	enzymes	770:776	arg1	Tryp					789:792	Tryp	789:792	Tryp	789:792	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	41	dep	enzymes	770:776	arg1	Pep					799:801	Pep	799:801	Pep	799:801	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	41	dep	enzymes	770:776	arg1	LPS					784:786	LPS	784:786	LPS	784:786	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	41	dep	enzymes	770:776	arg1	AMS					779:781	AMS	779:781	AMS	779:781	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	41	dep	enzymes	770:776	arg1	enzymes					770:776	digestive enzymes	760:776	digestive enzymes (AMS, LPS, Tryp, and Pep)	760:802	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	4	42	dep	diets	523:527	arg1	0 g/kg					564:569	0 g/kg	564:569	0 g/kg (Control)	564:579	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	42	dep	diets	523:527	arg1	50 g/kg					615:621	50 g/kg	615:621	50 g/kg (RS3)	615:627	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	42	dep	diets	523:527	arg1	Control					572:578	Control	572:578	Control	572:578	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	42	dep	diets	523:527	arg1	30 g/kg					597:603	30 g/kg	597:603	30 g/kg (RS2)	597:609	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	42	dep	diets	523:527	arg1	RS3					624:626	RS3	624:626	RS3	624:626	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	42	dep	diets	523:527	arg1	10 g/kg					582:588	10 g/kg	582:588	10 g/kg (RS1)	582:594	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	42	dep	diets	523:527	arg1	diets					523:527	diets	523:527	diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3)	523:627	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	42	dep	diets	523:527	arg1	RS2					606:608	RS2	606:608	RS2	606:608	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	4	42	dep	diets	523:527	arg1	RS1					591:593	RS1	591:593	RS1	591:593	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
31015554	3	43	theme	microbial	392:400	arg1	composition					402:412	the microbial composition	388:412	the microbial composition	388:412	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	0	44	from	immunity	50:57	arg1	response					86:93	response	86:93	response to dietary resistant starch	86:121	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	6	45	dep	genes	895:899	arg1	proPO					902:906	proPO	902:906	proPO	902:906	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	Muc-19					966:971	Muc-19	966:971	Muc-19	966:971	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	Muc-5AC					945:951	Muc-5AC	945:951	Muc-5AC	945:951	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	ALF					909:911	ALF	909:911	ALF	909:911	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	Trx					926:928	Trx	926:928	Trx	926:928	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	Muc-1					931:935	Muc-1	931:935	Muc-1	931:935	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	Muc-5B					954:959	Muc-5B	954:959	Muc-5B	954:959	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	genes					895:899	immune-related genes	880:899	immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19)	880:972	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	Lys					914:916	Lys	914:916	Lys	914:916	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	HSP70					919:923	HSP70	919:923	HSP70	919:923	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	45	dep	genes	895:899	arg1	Muc-2					938:942	Muc-2	938:942	Muc-2	938:942	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	9	46	dep	bacteria	1275:1282	arg1	Mesoflavibacter					1314:1328	Mesoflavibacter	1314:1328	Mesoflavibacter	1314:1328	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	46	dep	bacteria	1275:1282	arg1	Mameliella					1335:1344	Mameliella	1335:1344	Mameliella	1335:1344	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	46	dep	bacteria	1275:1282	arg1	Ruegeria					1296:1303	Ruegeria	1296:1303	Ruegeria	1296:1303	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	46	dep	bacteria	1275:1282	arg1	bacteria					1275:1282	the beneficial bacteria	1260:1282	the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella)	1260:1345	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	3	47	from	effects	363:369	arg1	composition					402:412	the microbial composition	388:412	the microbial composition	388:412	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	3	47	from	effects	363:369	arg1	intestine					463:471	the intestine	459:471	the intestine of Litopenaeus vannamei	459:495	In this study, we investigated the the effects of dietary RS on the microbial composition, and digestive and immune-related indices in the intestine of Litopenaeus vannamei.					
31015554	10	48	theme	SCFAs	1696:1700	arg1	contents					1702:1709	SCFAs contents	1696:1709	SCFAs contents	1696:1709	Our results revealed that dietary RS could improve the intestine health of L. vannamei, probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.					
31015554	6	49	dep	enzymes	815:821	arg1	NO					846:847	NO	846:847	NO	846:847	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	49	dep	enzymes	815:821	arg1	PO					824:825	PO	824:825	PO	824:825	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	49	dep	enzymes	815:821	arg1	enzymes					815:821	immune enzymes	808:821	immune enzymes (PO, T-AOC, T-NOS, and NO)	808:848	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	49	dep	enzymes	815:821	arg1	T-NOS					835:839	T-NOS	835:839	T-NOS	835:839	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	1	50	contain	has	185:187	arg1	constituent					151:161	a constituent	149:161	a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals	149:257	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	1	50	contain	has	185:187	arg1	starch					134:139	Resistant starch	124:139	Resistant starch (RS)	124:144	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	1	50	contain	has	185:187	arg2	effects					200:206	beneficial effects	189:206	beneficial effects	189:206	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	2	51	theme	intestine	295:303	arg1	health					305:310	shrimp intestine health	288:310	shrimp intestine health	288:310	However, the roles of RS on shrimp intestine health is unknown.					
31015554	7	52	theme	acids	1055:1059	arg1	contents					1069:1076	the short-chain fatty acids (SCFAs) contents	1033:1076	the short-chain fatty acids (SCFAs) contents	1033:1076	A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial.					
31015554	9	53	theme	genus	1247:1251	arg1	level					1253:1257	the genus level	1243:1257	the genus level	1243:1257	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	2	54	theme	shrimp	288:293	arg1	health					305:310	shrimp intestine health	288:310	shrimp intestine health	288:310	However, the roles of RS on shrimp intestine health is unknown.					
31015554	1	55	theme	beneficial	189:198	arg1	effects					200:206	beneficial effects	189:206	beneficial effects	189:206	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	9	56	theme	potential	1450:1458	arg1	Formosa					1471:1477	Formosa	1471:1477	Formosa	1471:1477	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	56	theme	potential	1450:1458	arg1	pathogens					1460:1468	potential pathogens	1450:1468	potential pathogens (Formosa and Pseudoalteromonas)	1450:1500	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	9	56	theme	potential	1450:1458	arg1	Pseudoalteromonas					1483:1499	Pseudoalteromonas	1483:1499	Pseudoalteromonas	1483:1499	At the genus level, the beneficial bacteria (Lutimonas, Ruegeria, Shimia, Mesoflavibacter, and Mameliella) were enriched, which might be involved in degrading toxins and producing beneficial metabolites; while potential pathogens (Formosa and Pseudoalteromonas) were decreased in response to dietary RS.					
31015554	1	57	theme	Resistant	124:132	arg1	constituent					151:161	a constituent	149:161	a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals	149:257	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	1	57	theme	Resistant	124:132	arg1	RS					142:143	RS	142:143	RS	142:143	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	1	57	theme	Resistant	124:132	arg1	starch					134:139	Resistant starch	124:139	Resistant starch (RS)	124:144	Resistant starch (RS) is a constituent of dietary fibre that has beneficial effects on the intestine physiological function of animals.					
31015554	7	58	theme	microbiome	977:986	arg1	analysis					988:995	A microbiome analysis	975:995	A microbiome analysis	975:995	A microbiome analysis indicated that dietary RS increased the short-chain fatty acids (SCFAs) contents and altered the composition of the intestine microbial.					
31015554	5	59	theme	mucosa	718:723	arg1	morphology					690:699	the morphology	686:699	the morphology of the intestine mucosa	686:723	The results showed that dietary RS improved the morphology of the intestine mucosa.					
31015554	6	60	theme	genes	895:899	arg1	levels					870:875	the expression levels	855:875	the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19)	855:972	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	10	61	theme	shrimp	1760:1765	arg1	immunity					1744:1751	immunity	1744:1751	immunity	1744:1751	Our results revealed that dietary RS could improve the intestine health of L. vannamei, probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.					
31015554	10	61	theme	shrimp	1760:1765	arg1	digestion					1730:1738	the digestion	1726:1738	the digestion	1726:1738	Our results revealed that dietary RS could improve the intestine health of L. vannamei, probably via modulating the intestine microbial composition and SCFAs contents, and enhancing the digestion and immunity of the shrimp.					
31015554	6	62	theme	enzymes	770:776	arg1	activity					748:755	the activity	744:755	the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO)	744:848	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	63	theme	enzymes	815:821	arg1	activity					748:755	the activity	744:755	the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO)	744:848	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	0	64	from	intestine	15:23	arg1	response					86:93	response	86:93	response to dietary resistant starch	86:121	Changes in the intestine microbial, digestion and immunity of Litopenaeus vannamei in response to dietary resistant starch.					
31015554	6	65	theme	immune-related	880:893	arg1	proPO					902:906	proPO	902:906	proPO	902:906	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	Muc-19					966:971	Muc-19	966:971	Muc-19	966:971	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	Muc-5AC					945:951	Muc-5AC	945:951	Muc-5AC	945:951	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	ALF					909:911	ALF	909:911	ALF	909:911	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	Trx					926:928	Trx	926:928	Trx	926:928	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	Muc-1					931:935	Muc-1	931:935	Muc-1	931:935	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	Muc-5B					954:959	Muc-5B	954:959	Muc-5B	954:959	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	genes					895:899	immune-related genes	880:899	immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19)	880:972	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	Lys					914:916	Lys	914:916	Lys	914:916	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	HSP70					919:923	HSP70	919:923	HSP70	919:923	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	6	65	theme	immune-related	880:893	arg1	Muc-2					938:942	Muc-2	938:942	Muc-2	938:942	RS also increased the activity of digestive enzymes (AMS, LPS, Tryp, and Pep) and immune enzymes (PO, T-AOC, T-NOS, and NO), and the expression levels of immune-related genes (proPO, ALF, Lys, HSP70, Trx, Muc-1, Muc-2, Muc-5AC, Muc-5B, and Muc-19).					
31015554	2	66	from	roles	273:277	arg1	health					305:310	shrimp intestine health	288:310	shrimp intestine health	288:310	However, the roles of RS on shrimp intestine health is unknown.					
31015554	4	67	theme	RS	560:561	arg1	levels					550:555	different levels	540:555	different levels of RS	540:561	The shrimp were fed with diets containing different levels of RS: 0 g/kg (Control), 10 g/kg (RS1), 30 g/kg (RS2) and 50 g/kg (RS3) for 56 days.					
30578906	7	0	theme	chitosan	1245:1252	arg1	treatment					1232:1240	the composite treatment	1218:1240	the composite treatment of chitosan with nano-TiO2	1218:1267	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	7	0	theme	chitosan	1245:1252	arg1	chitosan/nano-SiO2					1272:1289	chitosan/nano-SiO2	1272:1289	chitosan/nano-SiO2	1272:1289	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	7	1	theme	desirable	1301:1309	arg1	performance					1311:1321	desirable performance	1301:1321	desirable performance	1301:1321	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	7	2	with	chitosan/nano-SiO2	1272:1289	arg1	nano-TiO2					1259:1267	nano-TiO2	1259:1267	nano-TiO2	1259:1267	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	1	3	theme	chitosan/nano-SiO2	153:170	arg1	coating					172:178	chitosan/nano-SiO2 coating	153:178	chitosan/nano-SiO2 coating	153:178	In this study, the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films were prepared, their physico-chemical properties were determined.					
30578906	0	4	theme	chitosan/nano-SiO2	75:92	arg1	films					94:98	chitosan/nano-TiO2 and chitosan/nano-SiO2 films	52:98	chitosan/nano-TiO2 and chitosan/nano-SiO2 films	52:98	Preservation of Ginkgo biloba seeds by coating with chitosan/nano-TiO2 and chitosan/nano-SiO2 films.					
30578906	7	5	theme	quality	1521:1527	arg1	enhancement					1501:1511	the enhancement	1497:1511	the enhancement of seed quality	1497:1527	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	1	6	theme	coating	172:178	arg1	films					180:184	the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films	116:184	the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films	116:184	In this study, the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films were prepared, their physico-chemical properties were determined.					
30578906	6	7	theme	storage	1197:1203	arg1	time					1205:1208	storage time	1197:1208	storage time	1197:1208	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	3	8	theme	nano-SiO2	391:399	arg1	particles					401:409	nano-TiO2 and nano-SiO2 particles	377:409	nano-TiO2 and nano-SiO2 particles	377:409	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	6	9	theme	enzymes	1025:1031	arg1	activities					989:998	Higher activities	982:998	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT)	982:1106	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	4	10	theme	superoxide	742:751	arg1	O2·-					760:763	O2·-	760:763	O2·-	760:763	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	10	theme	superoxide	742:751	arg1	anion					753:757	superoxide anion	742:757	superoxide anion (O2·-) production rate	742:780	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	11	theme	decay	644:648	arg1	rate					650:653	the decay rate	640:653	the decay rate	640:653	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	5	12	theme	G.	868:869	arg1	seeds					878:882	G. biloba seeds	868:882	G. biloba seeds coated with chitosan alone and chitosan/nanocomposite	868:936	After storage, the firmness of G. biloba seeds coated with chitosan alone and chitosan/nanocomposite were markedly higher than those of control.					
30578906	7	13	theme	seed	1516:1519	arg1	quality					1521:1527	seed quality	1516:1527	seed quality	1516:1527	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	8	14	theme	potential	1605:1613	arg1	method					1615:1620	a potential method	1603:1620	a potential method for commercial preservation	1603:1648	Coating G. biloba seeds with chitosan/nano-TiO2 or chitosan/nano-SiO2 is a potential method for commercial preservation.					
30578906	4	15	theme	respiration	672:682	arg1	rate					684:687	respiration rate	672:687	respiration rate	672:687	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	6	16	theme	superoxide	1061:1070	arg1	SOD					1083:1085	SOD	1083:1085	SOD	1083:1085	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	6	16	theme	superoxide	1061:1070	arg1	dismutase					1072:1080	superoxide dismutase	1061:1080	superoxide dismutase (SOD)	1061:1086	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	5	17	theme	seeds	878:882	arg1	higher					952:957	higher	952:957	higher	952:957	After storage, the firmness of G. biloba seeds coated with chitosan alone and chitosan/nanocomposite were markedly higher than those of control.					
30578906	5	17	theme	seeds	878:882	arg1	firmness					856:863	the firmness	852:863	the firmness of G. biloba seeds coated with chitosan alone and chitosan/nanocomposite	852:936	After storage, the firmness of G. biloba seeds coated with chitosan alone and chitosan/nanocomposite were markedly higher than those of control.					
30578906	3	18	theme	nano-TiO2	377:385	arg1	particles					401:409	nano-TiO2 and nano-SiO2 particles	377:409	nano-TiO2 and nano-SiO2 particles	377:409	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	3	19	dep	enhanced	431:438	arg1	improved					496:503	improved	496:503	improved the water-vapor and gas permeability	496:540	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	2	20	theme	seeds	291:295	arg1	preservation					261:272	Then the preservation	252:272	Then the preservation of Ginkgo biloba seeds coating with these films	252:320	Then the preservation of Ginkgo biloba seeds coating with these films were investigated during storage.					
30578906	3	21	theme	composite	473:481	arg1	films					483:487	the composite films	469:487	the composite films	469:487	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	6	22	theme	antioxidant	1013:1023	arg1	peroxidase					1043:1052	peroxidase	1043:1052	peroxidase (POD)	1043:1058	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	6	22	theme	antioxidant	1013:1023	arg1	enzymes					1025:1031	scavenger antioxidant enzymes	1003:1031	scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT)	1003:1106	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	6	22	theme	antioxidant	1013:1023	arg1	catalase					1093:1100	catalase	1093:1100	catalase (CAT)	1093:1106	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	6	22	theme	antioxidant	1013:1023	arg1	dismutase					1072:1080	superoxide dismutase	1061:1080	superoxide dismutase (SOD)	1061:1086	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	7	23	theme	biloba	1464:1469	arg1	seeds					1471:1475	G. biloba seeds	1461:1475	G. biloba seeds	1461:1475	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	6	24	dep	chitosan/nano-TiO2	1129:1146	arg1	the					1125:1127	the	1125:1127	the	1125:1127	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	4	25	theme	electrolyte	716:726	arg1	rate					736:739	electrolyte leakage rate	716:739	electrolyte leakage rate	716:739	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	3	26	theme	best	418:421	arg1	formula					423:429	the best formula	414:429	the best formula	414:429	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	4	27	theme	particles	601:609	arg1	incorporation					560:572	incorporation	560:572	incorporation of nano-TiO2 and nano-SiO2 particles	560:609	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	28	theme	production	766:775	arg1	rate					777:780	superoxide anion (O2·-) production rate	742:780	superoxide anion (O2·-) production rate	742:780	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	3	29	theme	particles	401:409	arg1	incorporation					360:372	The incorporation	356:372	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula	356:429	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	4	30	theme	nano-SiO2	591:599	arg1	particles					601:609	nano-SiO2 particles	591:609	nano-SiO2 particles	591:609	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	7	31	theme	seeds	1408:1412	arg1	firmness					1386:1393	the firmness	1382:1393	the firmness of G. biloba seeds	1382:1412	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	0	32	theme	Ginkgo	16:21	arg1	seeds					30:34	Ginkgo biloba seeds	16:34	Ginkgo biloba seeds	16:34	Preservation of Ginkgo biloba seeds by coating with chitosan/nano-TiO2 and chitosan/nano-SiO2 films.					
30578906	8	33	theme	commercial	1626:1635	arg1	preservation					1637:1648	commercial preservation	1626:1648	commercial preservation	1626:1648	Coating G. biloba seeds with chitosan/nano-TiO2 or chitosan/nano-SiO2 is a potential method for commercial preservation.					
30578906	7	34	theme	G.	1461:1462	arg1	seeds					1471:1475	G. biloba seeds	1461:1475	G. biloba seeds	1461:1475	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	1	35	theme	physico-chemical	207:222	arg1	properties					224:233	their physico-chemical properties	201:233	their physico-chemical properties	201:233	In this study, the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films were prepared, their physico-chemical properties were determined.					
30578906	4	36	theme	anion	753:757	arg1	rate					777:780	superoxide anion (O2·-) production rate	742:780	superoxide anion (O2·-) production rate	742:780	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	37	theme	ethylene	690:697	arg1	rate					710:713	ethylene production rate	690:713	ethylene production rate	690:713	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	7	38	theme	antioxidant	1437:1447	arg1	activity					1449:1456	the antioxidant activity	1433:1456	the antioxidant activity in G. biloba seeds	1433:1475	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	7	39	from	activity	1449:1456	arg1	seeds					1471:1475	G. biloba seeds	1461:1475	G. biloba seeds	1461:1475	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	3	40	theme	films	483:487	arg1	properties					455:464	the mechanical properties	440:464	the mechanical properties of the composite films	440:487	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	0	41	theme	seeds	30:34	arg1	Preservation					0:11	Preservation	0:11	Preservation of Ginkgo biloba seeds by coating with chitosan/nano-TiO2 and chitosan/nano-SiO2 films.	0:99	Preservation of Ginkgo biloba seeds by coating with chitosan/nano-TiO2 and chitosan/nano-SiO2 films.					
30578906	6	42	theme	Higher	982:987	arg1	activities					989:998	Higher activities	982:998	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT)	982:1106	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	3	43	theme	mechanical	444:453	arg1	properties					455:464	the mechanical properties	440:464	the mechanical properties of the composite films	440:487	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	0	44	theme	biloba	23:28	arg1	seeds					30:34	Ginkgo biloba seeds	16:34	Ginkgo biloba seeds	16:34	Preservation of Ginkgo biloba seeds by coating with chitosan/nano-TiO2 and chitosan/nano-SiO2 films.					
30578906	5	45	theme	biloba	871:876	arg1	seeds					878:882	G. biloba seeds	868:882	G. biloba seeds coated with chitosan alone and chitosan/nanocomposite	868:936	After storage, the firmness of G. biloba seeds coated with chitosan alone and chitosan/nanocomposite were markedly higher than those of control.					
30578906	3	46	theme	formula	423:429	arg1	incorporation					360:372	The incorporation	356:372	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula	356:429	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	6	47	theme	scavenger	1003:1011	arg1	peroxidase					1043:1052	peroxidase	1043:1052	peroxidase (POD)	1043:1058	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	6	47	theme	scavenger	1003:1011	arg1	enzymes					1025:1031	scavenger antioxidant enzymes	1003:1031	scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT)	1003:1106	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	6	47	theme	scavenger	1003:1011	arg1	catalase					1093:1100	catalase	1093:1100	catalase (CAT)	1093:1106	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	6	47	theme	scavenger	1003:1011	arg1	dismutase					1072:1080	superoxide dismutase	1061:1080	superoxide dismutase (SOD)	1061:1086	Higher activities of scavenger antioxidant enzymes including peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were enhanced in the chitosan/nano-TiO2 and chitosan/nano-SiO2 coating treatments during storage time.					
30578906	7	48	with	treatment	1232:1240	arg1	nano-TiO2					1259:1267	nano-TiO2	1259:1267	nano-TiO2	1259:1267	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	7	49	theme	mildew	1337:1342	arg1	shrinkage					1356:1364	shrinkage	1356:1364	shrinkage	1356:1364	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	7	49	theme	mildew	1337:1342	arg1	occurrence					1344:1353	mildew occurrence	1337:1353	mildew occurrence	1337:1353	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	7	50	theme	composite	1222:1230	arg1	treatment					1232:1240	the composite treatment	1218:1240	the composite treatment of chitosan with nano-TiO2	1218:1267	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	7	51	theme	G.	1398:1399	arg1	seeds					1408:1412	G. biloba seeds	1398:1412	G. biloba seeds	1398:1412	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	3	52	theme	gas	525:527	arg1	permeability					529:540	the water-vapor and gas permeability	505:540	permeability	529:540	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	4	53	theme	nano-TiO2	577:585	arg1	incorporation					560:572	incorporation	560:572	incorporation of nano-TiO2 and nano-SiO2 particles	560:609	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	2	54	theme	biloba	284:289	arg1	seeds					291:295	Ginkgo biloba seeds	277:295	Ginkgo biloba seeds coating with these films	277:320	Then the preservation of Ginkgo biloba seeds coating with these films were investigated during storage.					
30578906	1	55	theme	chitosan	120:127	arg1	films					180:184	the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films	116:184	the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films	116:184	In this study, the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films were prepared, their physico-chemical properties were determined.					
30578906	4	56	theme	shrinkage	656:664	arg1	rate					666:669	shrinkage rate	656:669	shrinkage rate	656:669	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	57	theme	malondialdehyde	806:820	arg1	content					828:834	malondialdehyde (MDA) content	806:834	malondialdehyde (MDA) content	806:834	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	8	58	theme	G.	1538:1539	arg1	seeds					1548:1552	G. biloba seeds	1538:1552	G. biloba seeds	1538:1552	Coating G. biloba seeds with chitosan/nano-TiO2 or chitosan/nano-SiO2 is a potential method for commercial preservation.					
30578906	2	59	theme	Ginkgo	277:282	arg1	seeds					291:295	Ginkgo biloba seeds	277:295	Ginkgo biloba seeds coating with these films	277:320	Then the preservation of Ginkgo biloba seeds coating with these films were investigated during storage.					
30578906	8	60	theme	biloba	1541:1546	arg1	seeds					1548:1552	G. biloba seeds	1538:1552	G. biloba seeds	1538:1552	Coating G. biloba seeds with chitosan/nano-TiO2 or chitosan/nano-SiO2 is a potential method for commercial preservation.					
30578906	3	61	theme	water-vapor	509:519	arg1	permeability					529:540	the water-vapor and gas permeability	505:540	permeability	529:540	The incorporation of nano-TiO2 and nano-SiO2 particles of the best formula enhanced the mechanical properties of the composite films, which improved the water-vapor and gas permeability.					
30578906	7	62	theme	biloba	1401:1406	arg1	seeds					1408:1412	G. biloba seeds	1398:1412	G. biloba seeds	1398:1412	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	0	63	with	coating	39:45	arg1	films					94:98	chitosan/nano-TiO2 and chitosan/nano-SiO2 films	52:98	chitosan/nano-TiO2 and chitosan/nano-SiO2 films	52:98	Preservation of Ginkgo biloba seeds by coating with chitosan/nano-TiO2 and chitosan/nano-SiO2 films.					
30578906	1	64	theme	chitosan/nano-TiO2	130:147	arg1	films					180:184	the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films	116:184	the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films	116:184	In this study, the chitosan, chitosan/nano-TiO2 and chitosan/nano-SiO2 coating films were prepared, their physico-chemical properties were determined.					
30578906	4	65	theme	production	699:708	arg1	rate					710:713	ethylene production rate	690:713	ethylene production rate	690:713	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	0	66	theme	chitosan/nano-TiO2	52:69	arg1	films					94:98	chitosan/nano-TiO2 and chitosan/nano-SiO2 films	52:98	chitosan/nano-TiO2 and chitosan/nano-SiO2 films	52:98	Preservation of Ginkgo biloba seeds by coating with chitosan/nano-TiO2 and chitosan/nano-SiO2 films.					
30578906	4	67	theme	content	828:834	arg1	rate					650:653	the decay rate	640:653	the decay rate	640:653	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	67	theme	content	828:834	arg1	rate					684:687	respiration rate	672:687	respiration rate	672:687	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	67	theme	content	828:834	arg1	accumulation					790:801	the accumulation	786:801	the accumulation of malondialdehyde (MDA) content	786:834	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	67	theme	content	828:834	arg1	rate					736:739	electrolyte leakage rate	716:739	electrolyte leakage rate	716:739	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	67	theme	content	828:834	arg1	rate					666:669	shrinkage rate	656:669	shrinkage rate	656:669	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	67	theme	content	828:834	arg1	rate					777:780	superoxide anion (O2·-) production rate	742:780	superoxide anion (O2·-) production rate	742:780	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	4	67	theme	content	828:834	arg1	rate					710:713	ethylene production rate	690:713	ethylene production rate	690:713	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
30578906	7	68	dep	exhibited	1291:1299	arg1	affect					1426:1431	affect	1426:1431	affect	1426:1431	Either the composite treatment of chitosan with nano-TiO2 or chitosan/nano-SiO2 exhibited desirable performance in inhibiting mildew occurrence, shrinkage and maintaining the firmness of G. biloba seeds, positively affect the antioxidant activity in G. biloba seeds, thereby leading to the enhancement of seed quality.					
30578906	4	69	theme	leakage	728:734	arg1	rate					736:739	electrolyte leakage rate	716:739	electrolyte leakage rate	716:739	Chitosan coating incorporation of nano-TiO2 and nano-SiO2 particles could significantly decrease the decay rate, shrinkage rate, respiration rate, ethylene production rate, electrolyte leakage rate, superoxide anion (O2·-) production rate and the accumulation of malondialdehyde (MDA) content.					
31300688	0	0	theme	barrier	96:102	arg1	function					104:111	effective barrier function	86:111	effective barrier function	86:111	Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.					
31300688	4	1	theme	cell	867:870	arg1	niche					872:876	stem cell niche	862:876	stem cell niche	862:876	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	3	2	theme	simple	491:496	arg1	method					498:503	a simple method	489:503	a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays	489:679	Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays.					
31300688	0	3	theme	effective	86:94	arg1	function					104:111	effective barrier function	86:111	effective barrier function	86:111	Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.					
31300688	4	4	theme	stem	862:865	arg1	niche					872:876	stem cell niche	862:876	stem cell niche	862:876	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	2	5	theme	apical	351:356	arg1	side					358:361	the apical side	347:361	the apical side of the epithelium	347:379	However, their sphere-like geometry prevents access to the apical side of the epithelium, making them unsuitable for standard functional assays designed for flat cell monolayers.					
31300688	5	6	theme	differentiated	1029:1042	arg1	regions					1056:1062	differentiated villus-like regions	1029:1062	differentiated villus-like regions	1029:1062	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	7	7	dep	vivo-like	1420:1428	arg1	in					1417:1418	in	1417:1418	in	1417:1418	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	3	8	theme	epithelial	526:535	arg1	monolayers					537:546	epithelial monolayers	526:546	epithelial monolayers	526:546	Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays.					
31300688	1	9	theme	structural	258:267	arg1	levels					284:289	the structural and functional levels	254:289	the structural and functional levels	254:289	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	6	10	theme	accessible	1215:1224	arg1	surface					1233:1239	an accessible apical surface	1212:1239	an accessible apical surface	1212:1239	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	5	11	theme	proliferating	992:1004	arg1	domains					1017:1023	proliferating crypt-like domains	992:1023	proliferating crypt-like domains	992:1023	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	3	12	theme	monolayers	537:546	arg1	formation					513:521	the formation	509:521	the formation of epithelial monolayers	509:546	Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays.					
31300688	7	13	theme	culture	1362:1368	arg1	method					1370:1375	an up-to-date and novel culture method	1338:1375	an up-to-date and novel culture method for intestinal epithelium	1338:1401	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	6	14	theme	epithelial	1187:1196	arg1	barriers					1198:1205	functional epithelial barriers	1176:1205	functional epithelial barriers	1176:1205	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	6	14	theme	epithelial	1187:1196	arg1	characteristic					1127:1140	a unique characteristic	1118:1140	a unique characteristic	1118:1140	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	4	15	theme	medium	841:846	arg1	delivery					829:836	the basolateral delivery	813:836	the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors	813:910	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	7	16	theme	drug	1523:1526	arg1	absorption					1528:1537	high-throughput drug absorption	1507:1537	high-throughput drug absorption	1507:1537	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	4	17	theme	tissue	774:779	arg1	function					789:796	tissue barrier function	774:796	tissue barrier function	774:796	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	6	18	theme	electrical	1286:1295	arg1	resistance					1297:1306	physiologically relevant transepithelial electrical resistance	1245:1306	physiologically relevant transepithelial electrical resistance	1245:1306	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	4	19	link	myofibroblast-derived	882:902	arg1	factors					904:910	myofibroblast-derived factors	882:910	myofibroblast-derived factors	882:910	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	2	20	theme	flat	449:452	arg1	monolayers					459:468	flat cell monolayers	449:468	flat cell monolayers	449:468	However, their sphere-like geometry prevents access to the apical side of the epithelium, making them unsuitable for standard functional assays designed for flat cell monolayers.					
31300688	5	21	theme	crypt-like	1006:1015	arg1	domains					1017:1023	proliferating crypt-like domains	992:1023	proliferating crypt-like domains	992:1023	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	7	22	theme	cell	1430:1433	arg1	composition					1435:1445	an in vivo-like cell composition	1414:1445	an in vivo-like cell composition	1414:1445	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	0	23	theme	intestinal	15:24	arg1	monolayers					37:46	Self-organized intestinal epithelial monolayers	0:46	Self-organized intestinal epithelial monolayers in crypt and villus-like domains	0:79	Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.					
31300688	7	24	theme	intestinal	1381:1390	arg1	epithelium					1392:1401	intestinal epithelium	1381:1401	intestinal epithelium	1381:1401	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	7	25	theme	up-to-date	1341:1350	arg1	method					1370:1375	an up-to-date and novel culture method	1338:1375	an up-to-date and novel culture method for intestinal epithelium	1338:1401	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	5	26	theme	epithelial	955:964	arg1	types					971:975	major intestinal epithelial cell types	938:975	major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution	938:1112	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	1	27	theme	intestinal	189:198	arg1	biology					200:206	intestinal biology	189:206	intestinal biology	189:206	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	0	28	theme	Self-organized	0:13	arg1	monolayers					37:46	Self-organized intestinal epithelial monolayers	0:46	Self-organized intestinal epithelial monolayers in crypt and villus-like domains	0:79	Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.					
31300688	1	29	theme	functional	273:282	arg1	levels					284:289	the structural and functional levels	254:289	the structural and functional levels	254:289	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	1	30	from	levels	284:289	arg1	resemblance					221:231	their resemblance	215:231	their resemblance to in vivo tissue at the structural and functional levels	215:289	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	2	31	theme	sphere-like	307:317	arg1	geometry					319:326	their sphere-like geometry	301:326	their sphere-like geometry	301:326	However, their sphere-like geometry prevents access to the apical side of the epithelium, making them unsuitable for standard functional assays designed for flat cell monolayers.					
31300688	2	32	theme	epithelium	370:379	arg1	side					358:361	the apical side	347:361	the apical side of the epithelium	347:379	However, their sphere-like geometry prevents access to the apical side of the epithelium, making them unsuitable for standard functional assays designed for flat cell monolayers.					
31300688	6	33	theme	transepithelial	1270:1284	arg1	resistance					1297:1306	physiologically relevant transepithelial electrical resistance	1245:1306	physiologically relevant transepithelial electrical resistance	1245:1306	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	4	34	theme	basolateral	817:827	arg1	delivery					829:836	the basolateral delivery	813:836	the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors	813:910	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	1	35	theme	Intestinal	114:123	arg1	organoids					125:133	Intestinal organoids	114:133	Intestinal organoids	114:133	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	7	36	theme	culture	1476:1482	arg1	format					1484:1489	a tissue culture format	1467:1489	a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies	1467:1579	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	2	37	theme	functional	418:427	arg1	assays					429:434	standard functional assays	409:434	standard functional assays designed for flat cell monolayers	409:468	However, their sphere-like geometry prevents access to the apical side of the epithelium, making them unsuitable for standard functional assays designed for flat cell monolayers.					
31300688	0	38	theme	epithelial	26:35	arg1	monolayers					37:46	Self-organized intestinal epithelial monolayers	0:46	Self-organized intestinal epithelial monolayers in crypt and villus-like domains	0:79	Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.					
31300688	5	39	theme	cell	966:969	arg1	types					971:975	major intestinal epithelial cell types	938:975	major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution	938:1112	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	1	40	dep	in	162:163	arg1	vitro					165:169	vitro	165:169	vitro	165:169	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	7	41	theme	high-throughput	1507:1521	arg1	absorption					1528:1537	high-throughput drug absorption	1507:1537	high-throughput drug absorption	1507:1537	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	2	42	theme	standard	409:416	arg1	assays					429:434	standard functional assays	409:434	standard functional assays designed for flat cell monolayers	409:468	However, their sphere-like geometry prevents access to the apical side of the epithelium, making them unsuitable for standard functional assays designed for flat cell monolayers.					
31300688	0	43	theme	crypt	51:55	arg1	domains					73:79	crypt and villus-like domains	51:79	crypt and villus-like domains	51:79	Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.					
31300688	6	44	theme	functional	1176:1185	arg1	barriers					1198:1205	functional epithelial barriers	1176:1205	functional epithelial barriers	1176:1205	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	6	44	theme	functional	1176:1185	arg1	characteristic					1127:1140	a unique characteristic	1118:1140	a unique characteristic	1118:1140	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	3	45	theme	functional	648:657	arg1	assays					674:679	functional tissue barrier assays	648:679	functional tissue barrier assays	648:679	Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays.					
31300688	3	46	theme	type	589:592	arg1	composition					594:604	vivo-like cell type composition	574:604	vivo-like cell type composition	574:604	Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays.					
31300688	7	47	theme	interaction	1561:1571	arg1	studies					1573:1579	microbe-epithelium interaction studies	1542:1579	microbe-epithelium interaction studies	1542:1579	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	6	48	theme	unique	1120:1125	arg1	characteristic					1127:1140	a unique characteristic	1118:1140	a unique characteristic	1118:1140	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	6	48	theme	unique	1120:1125	arg1	barriers					1198:1205	functional epithelial barriers	1176:1205	functional epithelial barriers	1176:1205	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	7	49	theme	compatible	1491:1500	arg1	format					1484:1489	a tissue culture format	1467:1489	a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies	1467:1579	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	7	50	theme	vivo-like	1420:1428	arg1	composition					1435:1445	an in vivo-like cell composition	1414:1445	an in vivo-like cell composition	1414:1445	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	7	51	theme	tissue	1469:1474	arg1	format					1484:1489	a tissue culture format	1467:1489	a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies	1467:1579	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	0	52	theme	villus-like	61:71	arg1	domains					73:79	crypt and villus-like domains	51:79	crypt and villus-like domains	51:79	Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.					
31300688	1	53	dep	in	236:237	arg1	vivo					239:242	vivo	239:242	vivo	239:242	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	3	54	theme	vivo-like	574:582	arg1	composition					594:604	vivo-like cell type composition	574:604	vivo-like cell type composition	574:604	Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays.					
31300688	6	55	theme	epithelial	1149:1158	arg1	monolayers					1160:1169	these epithelial monolayers	1143:1169	these epithelial monolayers	1143:1169	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	4	56	theme	monolayer	710:718	arg1	spreading					720:728	epithelial monolayer spreading	699:728	epithelial monolayer spreading	699:728	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	2	57	theme	cell	454:457	arg1	monolayers					459:468	flat cell monolayers	449:468	flat cell monolayers	449:468	However, their sphere-like geometry prevents access to the apical side of the epithelium, making them unsuitable for standard functional assays designed for flat cell monolayers.					
31300688	4	58	theme	myofibroblast-derived	882:902	arg1	factors					904:910	myofibroblast-derived factors	882:910	myofibroblast-derived factors	882:910	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	5	59	theme	in	1088:1089	arg1	distribution					1101:1112	the in vivo cell distribution	1084:1112	the in vivo cell distribution	1084:1112	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	3	60	theme	cell	584:587	arg1	composition					594:604	vivo-like cell type composition	574:604	vivo-like cell type composition	574:604	Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays.					
31300688	5	61	contain	contain	930:936	arg1	monolayers					919:928	These monolayers	913:928	These monolayers	913:928	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	5	61	contain	contain	930:936	arg2	types					971:975	major intestinal epithelial cell types	938:975	major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution	938:1112	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	4	62	theme	epithelial	699:708	arg1	spreading					720:728	epithelial monolayer spreading	699:728	epithelial monolayer spreading	699:728	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	7	63	theme	microbe-epithelium	1542:1559	arg1	studies					1573:1579	microbe-epithelium interaction studies	1542:1579	microbe-epithelium interaction studies	1542:1579	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	6	64	theme	resistance	1297:1306	arg1	values					1308:1313	an accessible apical surface and physiologically relevant transepithelial electrical resistance values	1212:1313	an accessible apical surface and physiologically relevant transepithelial electrical resistance values	1212:1313	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	5	65	theme	major	938:942	arg1	types					971:975	major intestinal epithelial cell types	938:975	major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution	938:1112	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	4	66	theme	substrate	747:755	arg1	stiffness					757:765	the substrate stiffness	743:765	the substrate stiffness	743:765	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	6	67	theme	apical	1226:1231	arg1	surface					1233:1239	an accessible apical surface	1212:1239	an accessible apical surface	1212:1239	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	3	68	theme	tissue	659:664	arg1	assays					674:679	functional tissue barrier assays	648:679	functional tissue barrier assays	648:679	Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays.					
31300688	4	69	theme	barrier	781:787	arg1	function					789:796	tissue barrier function	774:796	tissue barrier function	774:796	In our approach, epithelial monolayer spreading is driven by the substrate stiffness, while tissue barrier function is achieved by the basolateral delivery of medium enriched with stem cell niche and myofibroblast-derived factors.					
31300688	5	70	theme	intestinal	944:953	arg1	types					971:975	major intestinal epithelial cell types	938:975	major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution	938:1112	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	5	71	theme	cell	1096:1099	arg1	distribution					1101:1112	the in vivo cell distribution	1084:1112	the in vivo cell distribution	1084:1112	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	1	72	theme	in	236:237	arg1	tissue					244:249	in vivo tissue	236:249	in vivo tissue	236:249	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	3	73	theme	barrier	666:672	arg1	assays					674:679	functional tissue barrier assays	648:679	functional tissue barrier assays	648:679	Here, we describe a simple method for the formation of epithelial monolayers that recapitulates the in vivo-like cell type composition and organization and that is suitable for functional tissue barrier assays.					
31300688	5	74	theme	villus-like	1044:1054	arg1	regions					1056:1062	differentiated villus-like regions	1029:1062	differentiated villus-like regions	1029:1062	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	1	75	theme	powerful	153:160	arg1	tool					171:174	a powerful in vitro tool	151:174	a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels	151:289	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	6	76	theme	surface	1233:1239	arg1	values					1308:1313	an accessible apical surface and physiologically relevant transepithelial electrical resistance values	1212:1313	an accessible apical surface and physiologically relevant transepithelial electrical resistance values	1212:1313	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	5	77	dep	in	1088:1089	arg1	vivo					1091:1094	vivo	1091:1094	vivo	1091:1094	These monolayers contain major intestinal epithelial cell types organized into proliferating crypt-like domains and differentiated villus-like regions, closely resembling the in vivo cell distribution.					
31300688	0	78	from	monolayers	37:46	arg1	domains					73:79	crypt and villus-like domains	51:79	crypt and villus-like domains	51:79	Self-organized intestinal epithelial monolayers in crypt and villus-like domains show effective barrier function.					
31300688	6	79	theme	relevant	1261:1268	arg1	resistance					1297:1306	physiologically relevant transepithelial electrical resistance	1245:1306	physiologically relevant transepithelial electrical resistance	1245:1306	As a unique characteristic, these epithelial monolayers form functional epithelial barriers with an accessible apical surface and physiologically relevant transepithelial electrical resistance values.					
31300688	7	80	theme	novel	1356:1360	arg1	method					1370:1375	an up-to-date and novel culture method	1338:1375	an up-to-date and novel culture method for intestinal epithelium	1338:1401	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	1	81	theme	in	162:163	arg1	tool					171:174	a powerful in vitro tool	151:174	a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels	151:289	Intestinal organoids have emerged as a powerful in vitro tool for studying intestinal biology due to their resemblance to in vivo tissue at the structural and functional levels.					
31300688	7	82	with	compatible	1491:1500	arg1	studies					1573:1579	microbe-epithelium interaction studies	1542:1579	microbe-epithelium interaction studies	1542:1579	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31300688	7	82	with	compatible	1491:1500	arg1	absorption					1528:1537	high-throughput drug absorption	1507:1537	high-throughput drug absorption	1507:1537	Our technology offers an up-to-date and novel culture method for intestinal epithelium, providing an in vivo-like cell composition and distribution in a tissue culture format compatible with high-throughput drug absorption or microbe-epithelium interaction studies.					
31221054	3	0	theme	observational	335:347	arg1	study					349:353	This observational study	330:353	This observational study	330:353	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	0	1	from	Stroke	22:27	arg1	Patients					32:39	Patients	32:39	Patients With Atrial Fibrillation	32:64	Anticoagulation After Stroke in Patients With Atrial Fibrillation.					
31221054	5	2	theme	systemic	734:741	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	2	theme	systemic	734:741	arg1	embolism					743:750	systemic embolism	734:750	systemic embolism	734:750	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	0	3	with	Patients	32:39	arg1	Fibrillation					53:64	Atrial Fibrillation	46:64	Atrial Fibrillation	46:64	Anticoagulation After Stroke in Patients With Atrial Fibrillation.					
31221054	10	4	theme	Conclusions-	1515:1526	arg1	findings					1532:1539	Conclusions- Our findings	1515:1539	Conclusions- Our findings	1515:1539	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	10	5	theme	ischemic	1630:1637	arg1	recurrence					1639:1648	early ischemic recurrence	1624:1648	early ischemic recurrence	1624:1648	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	9	6	theme	ischemic	1380:1387	arg1	points					1496:1501	ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points	1380:1501	ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately	1380:1512	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	7	theme	end	1492:1494	arg1	points					1496:1501	ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points	1380:1501	ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately	1380:1512	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	6	8	theme	patients	878:885	arg1	Results-					861:868	Results-	861:868	Results- Of 1810 patients who initiated oral anticoagulant therapy, 371 (20%)	861:937	Results- Of 1810 patients who initiated oral anticoagulant therapy, 371 (20%) underwent bridging therapy with full-dose low-molecular-weight heparin.					
31221054	7	9	theme	bridging	1097:1104	arg1	therapy					1106:1112	bridging therapy	1097:1112	bridging therapy	1097:1112	Older age and the presence of leukoaraiosis were inversely correlated with the use of bridging therapy.					
31221054	10	10	theme	nonbridged	1695:1704	arg1	patients					1706:1713	nonbridged patients	1695:1713	nonbridged patients	1695:1713	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	6	11	theme	bridging	949:956	arg1	therapy					958:964	bridging therapy	949:964	bridging therapy with full-dose low-molecular-weight heparin	949:1008	Results- Of 1810 patients who initiated oral anticoagulant therapy, 371 (20%) underwent bridging therapy with full-dose low-molecular-weight heparin.					
31221054	4	12	from	data	573:576	arg1	RAF					611:613	the prospective RAF and RAF-NOACs studies	595:635	RAF	611:613	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	4	12	from	data	573:576	arg1	studies					629:635	the prospective RAF and RAF-NOACs studies	595:635	studies	629:635	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	3	13	dep	profiles	389:396	arg1	1					373:373	1	373:373	1	373:373	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	9	14	dep	ischemic	1380:1387	arg1	CI					1411:1412	2.2; 95% CI	1402:1412	2.2; 95% CI	1402:1412	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	14	dep	ischemic	1380:1387	arg1	odds					1450:1453	odds ratio, 2.4	1450:1464	odds ratio, 2.4	1450:1464	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	14	dep	ischemic	1380:1387	arg1	odds					1390:1393	odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005	1390:1430	odds	1390:1393	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	2	15	theme	clinical	311:318	arg1	settings					320:327	clinical settings	311:327	clinical settings	311:327	However, this practice is common in clinical settings.					
31221054	9	16	theme	multivariable	1238:1250	arg1	analysis					1252:1259	multivariable analysis	1238:1259	multivariable analysis	1238:1259	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	5	17	theme	primary	642:648	arg1	composite					677:685	the composite	673:685	the composite of ischemic stroke	673:704	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	17	theme	primary	642:648	arg1	bleeding					808:815	bleeding	808:815	bleeding	808:815	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	17	theme	primary	642:648	arg1	bleeding					774:781	bleeding	774:781	bleeding	774:781	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	17	theme	primary	642:648	arg1	attack					726:731	transient ischemic attack	707:731	transient ischemic attack	707:731	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	17	theme	primary	642:648	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	17	theme	primary	642:648	arg1	embolism					743:750	systemic embolism	734:750	systemic embolism	734:750	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	17	theme	primary	642:648	arg1	stroke					699:704	ischemic stroke	690:704	ischemic stroke	690:704	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	3	18	dep	outcomes	474:481	arg1	2					457:457	2	457:457	2	457:457	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	9	19	theme	composite	1303:1311	arg1	ratio					1329:1333	odds ratio	1324:1333	odds ratio	1324:1333	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	19	theme	composite	1303:1311	arg1	point					1317:1321	the composite end point	1299:1321	the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001)	1299:1366	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	20	theme	%	1343:1343	arg1	CI					1345:1346	95% CI	1341:1346	95% CI	1341:1346	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	5	21	theme	transient	707:715	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	21	theme	transient	707:715	arg1	attack					726:731	transient ischemic attack	707:731	transient ischemic attack	707:731	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	3	22	dep	patients	401:408	arg1	receiving					410:418	receiving	410:418	receiving	410:418	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	3	22	dep	patients	401:408	arg1	receiving					428:436	receiving	428:436	receiving bridging therapy, (2) overall group outcomes, and (3) outcomes	428:499	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	4	23	theme	prospective	599:609	arg1	RAF					611:613	the prospective RAF and RAF-NOACs studies	595:635	RAF	611:613	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	9	24	theme	%	1409:1409	arg1	CI					1411:1412	2.2; 95% CI	1402:1412	2.2; 95% CI	1402:1412	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	24	theme	%	1409:1409	arg1	odds					1390:1393	odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005	1390:1430	odds	1390:1393	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	1	25	theme	acute	182:186	arg1	patients					209:216	acute cardioembolic stroke patients	182:216	acute cardioembolic stroke patients	182:216	Background and Purpose- Bridging therapy with low-molecular-weight heparin reportedly leads to a worse outcome for acute cardioembolic stroke patients because of a higher incidence of intracerebral bleeding.					
31221054	5	26	theme	ischemic	717:724	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	26	theme	ischemic	717:724	arg1	attack					726:731	transient ischemic attack	707:731	transient ischemic attack	707:731	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	9	27	theme	odds	1324:1327	arg1	ratio					1329:1333	odds ratio	1324:1333	odds ratio	1324:1333	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	27	theme	odds	1324:1327	arg1	point					1317:1321	the composite end point	1299:1321	the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001)	1299:1366	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	1	28	theme	cardioembolic	188:200	arg1	patients					209:216	acute cardioembolic stroke patients	182:216	acute cardioembolic stroke patients	182:216	Background and Purpose- Bridging therapy with low-molecular-weight heparin reportedly leads to a worse outcome for acute cardioembolic stroke patients because of a higher incidence of intracerebral bleeding.					
31221054	4	29	from	studies	629:635	arg1	patients					581:588	patients	581:588	patients from the prospective RAF and RAF-NOACs studies	581:635	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	4	29	from	studies	629:635	arg1	data					573:576	data	573:576	data of patients from the prospective RAF and RAF-NOACs studies	573:635	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	1	30	theme	stroke	202:207	arg1	patients					209:216	acute cardioembolic stroke patients	182:216	acute cardioembolic stroke patients	182:216	Background and Purpose- Bridging therapy with low-molecular-weight heparin reportedly leads to a worse outcome for acute cardioembolic stroke patients because of a higher incidence of intracerebral bleeding.					
31221054	3	31	theme	anticoagulant	526:538	arg1	type					518:521	the type	514:521	the type of anticoagulant prescribed	514:549	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	6	32	theme	oral	901:904	arg1	therapy					920:926	oral anticoagulant therapy	901:926	oral anticoagulant therapy	901:926	Results- Of 1810 patients who initiated oral anticoagulant therapy, 371 (20%) underwent bridging therapy with full-dose low-molecular-weight heparin.					
31221054	9	33	theme	end	1313:1315	arg1	ratio					1329:1333	odds ratio	1324:1333	odds ratio	1324:1333	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	33	theme	end	1313:1315	arg1	point					1317:1321	the composite end point	1299:1321	the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001)	1299:1366	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	34	theme	1.3-3.9	1415:1421	arg1	CI					1411:1412	2.2; 95% CI	1402:1412	2.2; 95% CI	1402:1412	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	34	theme	1.3-3.9	1415:1421	arg1	odds					1390:1393	odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005	1390:1430	odds	1390:1393	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	8	35	theme	bridged	1125:1131	arg1	patients					1133:1140	Forty-two bridged patients	1115:1140	Forty-two bridged patients (11.3%)	1115:1148	Forty-two bridged patients (11.3%) reached the combined outcome versus 72 (5.0%) of the nonbridged patients (P=0.0001).					
31221054	8	35	theme	bridged	1125:1131	arg1	%					1147:1147	11.3%	1143:1147	11.3%	1143:1147	Forty-two bridged patients (11.3%) reached the combined outcome versus 72 (5.0%) of the nonbridged patients (P=0.0001).					
31221054	3	36	theme	bridging	438:445	arg1	therapy					447:453	bridging therapy	438:453	bridging therapy	438:453	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	8	37	theme	combined	1162:1169	arg1	outcome					1171:1177	the combined outcome	1158:1177	the combined outcome	1158:1177	Forty-two bridged patients (11.3%) reached the combined outcome versus 72 (5.0%) of the nonbridged patients (P=0.0001).					
31221054	10	38	theme	recurrence	1639:1648	arg1	risk					1616:1619	a higher risk	1607:1619	a higher risk of early ischemic recurrence and hemorrhagic transformation	1607:1679	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	9	39	dep	odds	1390:1393	arg1	ratio					1395:1399	ratio	1395:1399	ratio	1395:1399	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	39	dep	odds	1390:1393	arg1	P=0.005					1424:1430	P=0.005	1424:1430	P=0.005	1424:1430	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	8	40	theme	nonbridged	1203:1212	arg1	patients					1214:1221	the nonbridged patients	1199:1221	the nonbridged patients	1199:1221	Forty-two bridged patients (11.3%) reached the combined outcome versus 72 (5.0%) of the nonbridged patients (P=0.0001).					
31221054	3	41	theme	clinical	380:387	arg1	profiles					389:396	the clinical profiles	376:396	(1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes	372:499	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	9	42	dep	odds	1450:1453	arg1	ratio					1455:1459	ratio	1455:1459	ratio	1455:1459	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	42	dep	odds	1450:1453	arg1	CI					1471:1472	95% CI	1467:1472	95% CI	1467:1472	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	42	dep	odds	1450:1453	arg1	P=0.01					1484:1489	P=0.01	1484:1489	P=0.01	1484:1489	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	42	dep	odds	1450:1453	arg1	1.2-4.9					1475:1481	1.2-4.9	1475:1481	1.2-4.9	1475:1481	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	7	43	theme	leukoaraiosis	1041:1053	arg1	age					1017:1019	Older age	1011:1019	Older age	1011:1019	Older age and the presence of leukoaraiosis were inversely correlated with the use of bridging therapy.					
31221054	7	43	theme	leukoaraiosis	1041:1053	arg1	presence					1029:1036	the presence	1025:1036	the presence of leukoaraiosis	1025:1053	Older age and the presence of leukoaraiosis were inversely correlated with the use of bridging therapy.					
31221054	9	44	dep	ratio	1329:1333	arg1	1.4-3.7					1349:1355	1.4-3.7	1349:1355	1.4-3.7	1349:1355	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	44	dep	ratio	1329:1333	arg1	CI					1345:1346	95% CI	1341:1346	95% CI	1341:1346	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	44	dep	ratio	1329:1333	arg1	P<0.0001					1358:1365	P<0.0001	1358:1365	P<0.0001	1358:1365	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	9	44	dep	ratio	1329:1333	arg1	2.3					1336:1338	2.3	1336:1338	2.3	1336:1338	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	1	45	theme	low-molecular-weight	113:132	arg1	heparin					134:140	low-molecular-weight heparin	113:140	low-molecular-weight heparin	113:140	Background and Purpose- Bridging therapy with low-molecular-weight heparin reportedly leads to a worse outcome for acute cardioembolic stroke patients because of a higher incidence of intracerebral bleeding.					
31221054	4	46	from	RAF	611:613	arg1	patients					581:588	patients	581:588	patients from the prospective RAF and RAF-NOACs studies	581:635	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	4	46	from	RAF	611:613	arg1	data					573:576	data	573:576	data of patients from the prospective RAF and RAF-NOACs studies	573:635	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	3	47	theme	overall	460:466	arg1	outcomes					474:481	overall group outcomes	460:481	(2) overall group outcomes	456:481	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	4	48	dep	analyzed	564:571	arg1	Methods-					552:559	Methods-	552:559	Methods-	552:559	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	10	49	theme	early	1624:1628	arg1	recurrence					1639:1648	early ischemic recurrence	1624:1648	early ischemic recurrence	1624:1648	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	5	50	theme	acute	847:851	arg1	stroke					853:858	the acute stroke	843:858	the acute stroke	843:858	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	4	51	theme	RAF-NOACs	619:627	arg1	studies					629:635	the prospective RAF and RAF-NOACs studies	595:635	studies	629:635	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	5	52	theme	ischemic	690:697	arg1	stroke					699:704	ischemic stroke	690:704	ischemic stroke	690:704	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	1	53	theme	higher	231:236	arg1	incidence					238:246	a higher incidence	229:246	a higher incidence of intracerebral bleeding	229:272	Background and Purpose- Bridging therapy with low-molecular-weight heparin reportedly leads to a worse outcome for acute cardioembolic stroke patients because of a higher incidence of intracerebral bleeding.					
31221054	3	54	theme	patients	401:408	arg1	profiles					389:396	the clinical profiles	376:396	(1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes	372:499	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	5	55	theme	stroke	699:704	arg1	composite					677:685	the composite	673:685	the composite of ischemic stroke	673:704	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	55	theme	stroke	699:704	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	55	theme	stroke	699:704	arg1	stroke					699:704	ischemic stroke	690:704	ischemic stroke	690:704	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	55	theme	stroke	699:704	arg1	attack					726:731	transient ischemic attack	707:731	transient ischemic attack	707:731	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	55	theme	stroke	699:704	arg1	embolism					743:750	systemic embolism	734:750	systemic embolism	734:750	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	10	56	theme	transformation	1666:1679	arg1	risk					1616:1619	a higher risk	1607:1619	a higher risk of early ischemic recurrence and hemorrhagic transformation	1607:1679	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	9	57	theme	hemorrhagic	1437:1447	arg1	points					1496:1501	ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points	1380:1501	ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately	1380:1512	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	0	58	theme	Atrial	46:51	arg1	Fibrillation					53:64	Atrial Fibrillation	46:64	Atrial Fibrillation	46:64	Anticoagulation After Stroke in Patients With Atrial Fibrillation.					
31221054	6	59	theme	anticoagulant	906:918	arg1	therapy					920:926	oral anticoagulant therapy	901:926	oral anticoagulant therapy	901:926	Results- Of 1810 patients who initiated oral anticoagulant therapy, 371 (20%) underwent bridging therapy with full-dose low-molecular-weight heparin.					
31221054	5	60	located	observed	817:824	arg2	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	60	located	observed	817:824	arg2	composite					677:685	the composite	673:685	the composite of ischemic stroke	673:704	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	60	located	observed	817:824	arg1	days					832:835	90 days	829:835	90 days after the acute stroke	829:858	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	5	60	located	observed	817:824	arg2	stroke					699:704	ischemic stroke	690:704	ischemic stroke	690:704	The primary outcome was defined as the composite of ischemic stroke, transient ischemic attack, systemic embolism, symptomatic cerebral bleeding, and major extracerebral bleeding observed at 90 days after the acute stroke.					
31221054	10	61	theme	higher	1609:1614	arg1	risk					1616:1619	a higher risk	1607:1619	a higher risk of early ischemic recurrence and hemorrhagic transformation	1607:1679	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	10	62	contain	have	1602:1605	arg1	patients					1554:1561	patients	1554:1561	patients receiving low-molecular-weight heparin	1554:1600	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	10	62	contain	have	1602:1605	arg2	risk					1616:1619	a higher risk	1607:1619	a higher risk of early ischemic recurrence and hemorrhagic transformation	1607:1679	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	9	63	theme	%	1469:1469	arg1	CI					1471:1472	95% CI	1467:1472	95% CI	1467:1472	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	10	64	theme	low-molecular-weight	1573:1592	arg1	heparin					1594:1600	low-molecular-weight heparin	1573:1600	low-molecular-weight heparin	1573:1600	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	0	65	dep	Anticoagulation	0:14	arg1	Stroke					22:27	Stroke	22:27	Stroke in Patients With Atrial Fibrillation	22:64	Anticoagulation After Stroke in Patients With Atrial Fibrillation.					
31221054	10	66	theme	hemorrhagic	1654:1664	arg1	transformation					1666:1679	hemorrhagic transformation	1654:1679	hemorrhagic transformation	1654:1679	Conclusions- Our findings suggest that patients receiving low-molecular-weight heparin have a higher risk of early ischemic recurrence and hemorrhagic transformation compared with nonbridged patients.					
31221054	9	67	theme	2.4	1462:1464	arg1	ratio					1455:1459	ratio	1455:1459	ratio	1455:1459	At multivariable analysis, bridging therapy was associated with the composite end point (odds ratio, 2.3; 95% CI, 1.4-3.7; P<0.0001), as well as ischemic (odds ratio, 2.2; 95% CI, 1.3-3.9; P=0.005) and hemorrhagic (odds ratio, 2.4; 95% CI, 1.2-4.9; P=0.01) end points separately.					
31221054	3	68	theme	group	468:472	arg1	outcomes					474:481	overall group outcomes	460:481	(2) overall group outcomes	456:481	This observational study aimed to compare (1) the clinical profiles of patients receiving and not receiving bridging therapy, (2) overall group outcomes, and (3) outcomes according to the type of anticoagulant prescribed.					
31221054	4	69	theme	patients	581:588	arg1	data					573:576	data	573:576	data of patients from the prospective RAF and RAF-NOACs studies	573:635	Methods- We analyzed data of patients from the prospective RAF and RAF-NOACs studies.					
31221054	7	70	theme	Older	1011:1015	arg1	age					1017:1019	Older age	1011:1019	Older age	1011:1019	Older age and the presence of leukoaraiosis were inversely correlated with the use of bridging therapy.					
31221054	6	71	theme	low-molecular-weight	981:1000	arg1	heparin					1002:1008	full-dose low-molecular-weight heparin	971:1008	full-dose low-molecular-weight heparin	971:1008	Results- Of 1810 patients who initiated oral anticoagulant therapy, 371 (20%) underwent bridging therapy with full-dose low-molecular-weight heparin.					
31221054	8	72	theme	patients	1214:1221	arg1	patients					1214:1221	the nonbridged patients	1199:1221	the nonbridged patients	1199:1221	Forty-two bridged patients (11.3%) reached the combined outcome versus 72 (5.0%) of the nonbridged patients (P=0.0001).					
31221054	8	72	theme	patients	1214:1221	arg1	P=0.0001					1224:1231	P=0.0001	1224:1231	P=0.0001	1224:1231	Forty-two bridged patients (11.3%) reached the combined outcome versus 72 (5.0%) of the nonbridged patients (P=0.0001).					
31221054	8	72	theme	patients	1214:1221	arg1	72					1186:1187	72	1186:1187	72	1186:1187	Forty-two bridged patients (11.3%) reached the combined outcome versus 72 (5.0%) of the nonbridged patients (P=0.0001).					
31221054	8	72	theme	patients	1214:1221	arg1	%					1193:1193	5.0%	1190:1193	5.0%	1190:1193	Forty-two bridged patients (11.3%) reached the combined outcome versus 72 (5.0%) of the nonbridged patients (P=0.0001).					
31221054	6	73	with	therapy	958:964	arg1	heparin					1002:1008	full-dose low-molecular-weight heparin	971:1008	full-dose low-molecular-weight heparin	971:1008	Results- Of 1810 patients who initiated oral anticoagulant therapy, 371 (20%) underwent bridging therapy with full-dose low-molecular-weight heparin.					
31221054	2	74	from	common	301:306	arg1	settings					320:327	clinical settings	311:327	clinical settings	311:327	However, this practice is common in clinical settings.					
31221054	6	75	theme	full-dose	971:979	arg1	heparin					1002:1008	full-dose low-molecular-weight heparin	971:1008	full-dose low-molecular-weight heparin	971:1008	Results- Of 1810 patients who initiated oral anticoagulant therapy, 371 (20%) underwent bridging therapy with full-dose low-molecular-weight heparin.					
31221054	7	76	theme	therapy	1106:1112	arg1	use					1090:1092	the use	1086:1092	the use of bridging therapy	1086:1112	Older age and the presence of leukoaraiosis were inversely correlated with the use of bridging therapy.					
31221054	1	77	with	therapy	100:106	arg1	heparin					134:140	low-molecular-weight heparin	113:140	low-molecular-weight heparin	113:140	Background and Purpose- Bridging therapy with low-molecular-weight heparin reportedly leads to a worse outcome for acute cardioembolic stroke patients because of a higher incidence of intracerebral bleeding.					
31221054	1	78	theme	worse	164:168	arg1	outcome					170:176	a worse outcome	162:176	a worse outcome for acute cardioembolic stroke patients	162:216	Background and Purpose- Bridging therapy with low-molecular-weight heparin reportedly leads to a worse outcome for acute cardioembolic stroke patients because of a higher incidence of intracerebral bleeding.					
31221054	2	79	from	settings	320:327	arg1	common					301:306	common	301:306	common	301:306	However, this practice is common in clinical settings.					
31221054	1	80	theme	bleeding	265:272	arg1	incidence					238:246	a higher incidence	229:246	a higher incidence of intracerebral bleeding	229:272	Background and Purpose- Bridging therapy with low-molecular-weight heparin reportedly leads to a worse outcome for acute cardioembolic stroke patients because of a higher incidence of intracerebral bleeding.					
30197141	10	0	from	contrast	1684:1691	arg1	weak					1774:1777	weak	1774:1777	weak	1774:1777	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	10	0	from	contrast	1684:1691	arg1	0.09					1789:1792	0.09	1789:1792	0.09	1789:1792	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	10	0	from	contrast	1684:1691	arg1	correlations					1706:1717	the genetic correlations	1694:1717	the genetic correlations between milk pH0 PPC and CY or coagulation traits	1694:1767	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	4	1	theme	Montbéliarde	764:775	arg1	cows					777:780	126,873 primiparous Montbéliarde cows	744:780	126,873 primiparous Montbéliarde cows	744:780	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	12	2	theme	improved	2025:2032	arg1	coagulation					2034:2044	improved coagulation	2025:2044	improved coagulation	2025:2044	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30197141	6	3	theme	herd	987:990	arg1	spectrometer					1005:1016	herd × test day × spectrometer	987:1016	herd × test day × spectrometer	987:1016	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	10	4	theme	milk	1727:1730	arg1	PPC					1736:1738	milk pH0 PPC	1727:1738	milk pH0 PPC	1727:1738	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	6	5	theme	residual	1136:1143	arg1	effects					1145:1151	the random additive genetic, permanent environmental, and residual effects	1078:1151	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	2	6	theme	MIR	313:315	arg1	spectra					317:323	MIR spectra	313:323	MIR spectra (as determined by comparison with reference measurements)	313:381	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	2	7	with	comparison	343:352	arg1	measurements					369:380	reference measurements	359:380	reference measurements	359:380	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	6	8	theme	month	1050:1054	arg1	effects					1145:1151	the random additive genetic, permanent environmental, and residual effects	1078:1151	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	6	8	theme	month	1050:1054	arg1	effects					976:982	the fixed effects	966:982	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	10	9	theme	coagulation	1750:1760	arg1	traits					1762:1767	CY or coagulation traits	1744:1767	traits	1762:1767	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	14	10	theme	coagulation	2396:2406	arg1	properties					2408:2417	milk coagulation properties	2391:2417	milk coagulation properties	2391:2417	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	14	11	used	used	2451:2454	arg2	predictions					2358:2368	MIR predictions	2354:2368	MIR predictions of cheese yields and milk coagulation properties	2354:2417	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	1	12	theme	mid-infrared	246:257	arg1	spectra					265:271	mid-infrared (MIR) spectra	246:271	mid-infrared (MIR) spectra	246:271	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	2	13	dep	pH	492:493	arg1	pH0PPC					496:501	pH0PPC	496:501	initial pH; pH0PPC	484:501	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	6	14	theme	year	1043:1046	arg1	month					1050:1054	year × month	1043:1054	year × month of calving	1043:1065	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	10	15	theme	CY	1744:1745	arg1	traits					1762:1767	CY or coagulation traits	1744:1767	traits	1762:1767	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	15	16	theme	MIR	2518:2520	arg1	spectra					2522:2528	MIR spectra	2518:2528	MIR spectra	2518:2528	Cheese-making traits, as predicted from MIR spectra, are moderately heritable and could be integrated into breeding objectives without additional phenotyping cost, thus creating an opportunity for efficient improvement via selection.					
30197141	1	17	theme	cooked	183:188	arg1	cheeses					190:196	pressed cooked cheeses	175:196	pressed cooked cheeses (PCC)	175:202	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	1	17	theme	cooked	183:188	arg1	PCC					199:201	PCC	199:201	PCC	199:201	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	15	18	theme	phenotyping	2624:2634	arg1	cost					2636:2639	additional phenotyping cost	2613:2639	additional phenotyping cost	2613:2639	Cheese-making traits, as predicted from MIR spectra, are moderately heritable and could be integrated into breeding objectives without additional phenotyping cost, thus creating an opportunity for efficient improvement via selection.					
30197141	8	19	contain	had	1369:1371	arg2	heritability					1384:1395	the lowest heritability	1373:1395	the lowest heritability (0.37 to 0.39)	1373:1410	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	8	19	contain	had	1369:1371	arg1	yields					1350:1355	cheese yields	1343:1355	cheese yields	1343:1355	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	8	19	contain	had	1369:1371	arg1	PPC					1365:1367	pH0 PPC	1361:1367	pH0 PPC	1361:1367	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	8	19	contain	had	1369:1371	arg2	0.39					1406:1409	0.39	1406:1409	0.39	1406:1409	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	6	20	theme	genetic	1098:1104	arg1	effects					1145:1151	the random additive genetic, permanent environmental, and residual effects	1078:1151	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	14	21	theme	yields	2380:2385	arg1	predictions					2358:2368	MIR predictions	2354:2368	MIR predictions of cheese yields and milk coagulation properties	2354:2417	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	11	22	theme	cheese-making	1830:1842	arg1	traits					1853:1858	cheese-making property traits	1830:1858	cheese-making property traits	1830:1858	The genetic relationships between cheese-making property traits and milk composition were moderate to high.					
30197141	9	23	theme	cheese	1537:1542	arg1	yield					1544:1548	cheese yield	1537:1548	cheese yield	1537:1548	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	6	24	theme	additive	1089:1096	arg1	effects					1145:1151	the random additive genetic, permanent environmental, and residual effects	1078:1151	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	12	25	theme	high	1919:1922	arg1	levels					1924:1929	high levels	1919:1929	high levels of proteins, fatty acids, Ca, P, and Mg in milk	1919:1977	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30197141	0	26	theme	Montbéliarde	128:139	arg1	cows					141:144	Montbéliarde cows	128:144	Montbéliarde cows	128:144	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	1	27	theme	soft	208:211	arg1	SC					222:223	SC	222:223	SC	222:223	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	1	27	theme	soft	208:211	arg1	cheeses					213:219	soft cheeses	208:219	soft cheeses (SC)	208:224	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	14	28	theme	MIR	2354:2356	arg1	predictions					2358:2368	MIR predictions	2354:2368	MIR predictions of cheese yields and milk coagulation properties	2354:2417	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	7	29	theme	property	1230:1237	arg1	traits					1239:1244	the 9 cheese-making property traits	1210:1244	the 9 cheese-making property traits analyzed	1210:1253	Heritability estimates varied between 0.37 and 0.48 for the 9 cheese-making property traits analyzed.					
30197141	9	30	theme	coagulation	1559:1569	arg1	traits					1571:1576	coagulation traits	1559:1576	coagulation traits	1559:1576	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	1	31	dep	mid-infrared	246:257	arg1	MIR					260:262	MIR	260:262	MIR	260:262	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	15	32	theme	efficient	2675:2683	arg1	improvement					2685:2695	efficient improvement	2675:2695	efficient improvement via selection	2675:2709	Cheese-making traits, as predicted from MIR spectra, are moderately heritable and could be integrated into breeding objectives without additional phenotyping cost, thus creating an opportunity for efficient improvement via selection.					
30197141	8	33	dep	ones	1284:1287	arg1	whereas					1335:1341	whereas	1335:1341	whereas	1335:1341	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	6	34	theme	repeatability	916:928	arg1	model					937:941	A univariate or bivariate repeatability animal model	890:941	model	937:941	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	6	35	theme	calving	1059:1065	arg1	stage					1019:1023	stage	1019:1023	stage of lactation	1019:1036	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	6	35	theme	calving	1059:1065	arg1	spectrometer					1005:1016	herd × test day × spectrometer	987:1016	herd × test day × spectrometer	987:1016	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	6	35	theme	calving	1059:1065	arg1	month					1050:1054	year × month	1043:1054	year × month of calving	1043:1065	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	3	36	theme	traits	544:549	arg1	determination					521:533	determination	521:533	determination of these traits	521:549	Coefficients of determination of these traits ranged between 0.54 and 0.89.					
30197141	1	37	theme	Cheese-making	147:159	arg1	properties					161:170	Cheese-making properties	147:170	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC)	147:224	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	9	38	theme	cheese	1587:1592	arg1	yields					1594:1599	cheese yields	1587:1599	cheese yields	1587:1599	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	8	39	theme	cheese	1343:1348	arg1	yields					1350:1355	cheese yields	1343:1355	cheese yields	1343:1355	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	4	40	theme	composition	612:622	arg1	lactose					662:668	lactose	662:668	lactose	662:668	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	40	theme	composition	612:622	arg1	protein					644:650	protein	644:650	protein	644:650	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	40	theme	composition	612:622	arg1	content					683:689	citrate content	675:689	citrate content	675:689	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	40	theme	composition	612:622	arg1	traits					624:629	milk composition traits	607:629	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content)	581:690	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	40	theme	composition	612:622	arg1	acid					638:641	fatty acid	632:641	fatty acid	632:641	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	0	41	theme	mid-infrared	84:95	arg1	spectra					97:103	mid-infrared spectra	84:103	mid-infrared spectra	84:103	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	14	42	theme	large	2309:2313	arg1	scale					2315:2319	a large scale	2307:2319	a large scale in Montbéliarde cows	2307:2340	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	10	43	dep	0.09	1789:1792	arg1	to					1786:1787	to	1786:1787	to	1786:1787	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	15	44	dep	traits	2492:2497	arg1	predicted					2503:2511	predicted	2503:2511	predicted from MIR spectra	2503:2528	Cheese-making traits, as predicted from MIR spectra, are moderately heritable and could be integrated into breeding objectives without additional phenotyping cost, thus creating an opportunity for efficient improvement via selection.					
30197141	2	45	dep	spectra	317:323	arg1	determined					329:338	determined	329:338	determined by comparison with reference measurements	329:380	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	9	46	theme	Strong	1413:1418	arg1	correlations					1438:1449	Strong favorable genetic correlations	1413:1449	Strong favorable genetic correlations	1413:1449	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	10	47	from	weak	1774:1777	arg1	contrast					1684:1691	contrast	1684:1691	contrast	1684:1691	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	6	48	used	used	947:950	arg2	model					937:941	A univariate or bivariate repeatability animal model	890:941	model	937:941	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	6	48	used	used	947:950	arg2	univariate					892:901	A univariate or bivariate repeatability animal model	890:941	univariate	892:901	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	5	49	theme	traits	863:868	arg1	parameters					843:852	the corresponding genetic parameters	817:852	the corresponding genetic parameters of these traits	817:868	Using this data set, we estimated the corresponding genetic parameters of these traits by REML procedures.					
30197141	2	50	theme	acidification	455:467	arg1	trait					469:473	1 acidification trait	453:473	1 acidification trait	453:473	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	12	51	theme	cheese	2007:2012	arg1	yields					2014:2019	better cheese yields	2000:2019	better cheese yields	2000:2019	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30197141	13	52	theme	coagulation	2260:2270	arg1	traits					2272:2277	coagulation traits	2260:2277	coagulation traits	2260:2277	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	0	53	theme	large	110:114	arg1	set					121:123	a large data set	108:123	a large data set of Montbéliarde cows	108:144	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	0	53	theme	large	110:114	arg1	cows					141:144	Montbéliarde cows	128:144	Montbéliarde cows	128:144	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	5	54	theme	REML	873:876	arg1	procedures					878:887	REML procedures	873:887	REML procedures	873:887	Using this data set, we estimated the corresponding genetic parameters of these traits by REML procedures.					
30197141	6	55	theme	fixed	970:974	arg1	effects					976:982	the fixed effects	966:982	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	2	56	theme	laboratory	402:411	arg1	yield					420:424	laboratory cheese yield	402:424	laboratory cheese yield	402:424	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	10	57	dep	weak	1774:1777	arg1	weak					1774:1777	weak	1774:1777	weak	1774:1777	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	10	57	dep	weak	1774:1777	arg1	0.09					1789:1792	0.09	1789:1792	0.09	1789:1792	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	10	57	dep	weak	1774:1777	arg1	correlations					1706:1717	the genetic correlations	1694:1717	the genetic correlations between milk pH0 PPC and CY or coagulation traits	1694:1767	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	0	58	theme	Genetic	0:6	arg1	parameters					8:17	Genetic parameters	0:17	Genetic parameters for cheese-making properties and milk composition	0:67	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	5	59	theme	genetic	835:841	arg1	parameters					843:852	the corresponding genetic parameters	817:852	the corresponding genetic parameters of these traits	817:868	Using this data set, we estimated the corresponding genetic parameters of these traits by REML procedures.					
30197141	9	60	theme	coagulation	1637:1647	arg1	traits					1649:1654	coagulation traits	1637:1654	coagulation traits measured for PCC and SC	1637:1678	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	15	61	theme	Cheese-making	2478:2490	arg1	traits					2492:2497	Cheese-making traits	2478:2497	Cheese-making traits	2478:2497	Cheese-making traits, as predicted from MIR spectra, are moderately heritable and could be integrated into breeding objectives without additional phenotyping cost, thus creating an opportunity for efficient improvement via selection.					
30197141	4	62	dep	traits	624:629	arg1	acid					638:641	fatty acid	632:641	fatty acid	632:641	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	62	dep	traits	624:629	arg1	lactose					662:668	lactose	662:668	lactose	662:668	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	62	dep	traits	624:629	arg1	protein					644:650	protein	644:650	protein	644:650	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	62	dep	traits	624:629	arg1	mineral					653:659	mineral	653:659	mineral	653:659	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	62	dep	traits	624:629	arg1	content					683:689	citrate content	675:689	citrate content	675:689	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	62	dep	traits	624:629	arg1	traits					624:629	milk composition traits	607:629	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content)	581:690	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	14	63	theme	genetic	2460:2466	arg1	analyses					2468:2475	genetic analyses	2460:2475	genetic analyses	2460:2475	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	9	64	theme	genetic	1430:1436	arg1	correlations					1438:1449	Strong favorable genetic correlations	1413:1449	Strong favorable genetic correlations	1413:1449	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	7	65	theme	Heritability	1154:1165	arg1	estimates					1167:1175	Heritability estimates	1154:1175	Heritability estimates	1154:1175	Heritability estimates varied between 0.37 and 0.48 for the 9 cheese-making property traits analyzed.					
30197141	2	66	theme	yield	420:424	arg1	traits					278:283	The traits	274:283	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements)	274:381	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	2	66	theme	yield	420:424	arg1	trait					469:473	1 acidification trait	453:473	1 acidification trait	453:473	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	2	66	theme	yield	420:424	arg1	measures					390:397	3 measures	388:397	3 measures of laboratory cheese yield	388:424	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	2	66	theme	yield	420:424	arg1	traits					441:446	5 coagulation traits	427:446	5 coagulation traits	427:446	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	0	67	theme	milk	52:55	arg1	composition					57:67	milk composition	52:67	milk composition	52:67	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	13	68	theme	cheese	2155:2160	arg1	yields					2162:2167	cheese yields	2155:2167	cheese yields	2155:2167	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	6	69	theme	spectrometer	1005:1016	arg1	effects					1145:1151	the random additive genetic, permanent environmental, and residual effects	1078:1151	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	6	69	theme	spectrometer	1005:1016	arg1	effects					976:982	the fixed effects	966:982	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	10	70	theme	genetic	1698:1704	arg1	weak					1774:1777	weak	1774:1777	weak	1774:1777	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	10	70	theme	genetic	1698:1704	arg1	0.09					1789:1792	0.09	1789:1792	0.09	1789:1792	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	10	70	theme	genetic	1698:1704	arg1	correlations					1706:1717	the genetic correlations	1694:1717	the genetic correlations between milk pH0 PPC and CY or coagulation traits	1694:1767	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	1	71	theme	cheeses	213:219	arg1	properties					161:170	Cheese-making properties	147:170	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC)	147:224	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	9	72	theme	absolute	1457:1464	arg1	values					1466:1471	absolute values	1457:1471	absolute values between 0.64 and 0.97	1457:1493	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	6	73	theme	day	999:1001	arg1	spectrometer					1005:1016	herd × test day × spectrometer	987:1016	herd × test day × spectrometer	987:1016	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	13	74	with	yields	2243:2248	arg1	traits					2272:2277	coagulation traits	2260:2277	coagulation traits	2260:2277	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	8	75	theme	highest	1298:1304	arg1	heritability					1306:1317	the highest heritability	1294:1317	the highest heritability (0.42 to 0.48)	1294:1332	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	8	75	theme	highest	1298:1304	arg1	0.48					1328:1331	0.48	1328:1331	0.48	1328:1331	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	14	76	from	scale	2315:2319	arg1	cows					2337:2340	Montbéliarde cows	2324:2340	Montbéliarde cows	2324:2340	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	13	77	theme	coagulation	2106:2116	arg1	traits					2118:2123	coagulation traits	2106:2123	coagulation traits	2106:2123	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	13	78	theme	lesser	2135:2140	arg1	extent					2142:2147	a lesser extent	2133:2147	a lesser extent	2133:2147	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	6	79	theme	×	992:992	arg1	spectrometer					1005:1016	herd × test day × spectrometer	987:1016	herd × test day × spectrometer	987:1016	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	10	80	theme	pH0	1732:1734	arg1	PPC					1736:1738	milk pH0 PPC	1727:1738	milk pH0 PPC	1727:1738	In contrast, the genetic correlations between milk pH0 PPC and CY or coagulation traits were weak (-0.08 to 0.09).					
30197141	13	81	theme	fatty	2178:2182	arg1	acids					2184:2188	fatty acids	2178:2188	fatty acids in milk	2178:2196	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	4	82	theme	primiparous	752:762	arg1	cows					777:780	126,873 primiparous Montbéliarde cows	744:780	126,873 primiparous Montbéliarde cows	744:780	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	14	83	theme	milk	2391:2394	arg1	properties					2408:2417	milk coagulation properties	2391:2417	milk coagulation properties	2391:2417	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	9	84	with	correlations	1438:1449	arg1	values					1466:1471	absolute values	1457:1471	absolute values between 0.64 and 0.97	1457:1493	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	11	85	theme	genetic	1800:1806	arg1	to					1895:1896	to	1895:1896	to	1895:1896	The genetic relationships between cheese-making property traits and milk composition were moderate to high.					
30197141	11	85	theme	genetic	1800:1806	arg1	relationships					1808:1820	The genetic relationships	1796:1820	The genetic relationships between cheese-making property traits and milk composition	1796:1879	The genetic relationships between cheese-making property traits and milk composition were moderate to high.					
30197141	11	85	theme	genetic	1800:1806	arg1	high					1898:1901	high	1898:1901	high	1898:1901	The genetic relationships between cheese-making property traits and milk composition were moderate to high.					
30197141	2	86	dep	PCC	479:481	arg1	pH					492:493	initial pH	484:493	initial pH; pH0PPC	484:501	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	11	87	dep	high	1898:1901	arg1	to					1895:1896	to	1895:1896	to	1895:1896	The genetic relationships between cheese-making property traits and milk composition were moderate to high.					
30197141	11	87	dep	high	1898:1901	arg1	relationships					1808:1820	The genetic relationships	1796:1820	The genetic relationships between cheese-making property traits and milk composition	1796:1879	The genetic relationships between cheese-making property traits and milk composition were moderate to high.					
30197141	11	87	dep	high	1898:1901	arg1	high					1898:1901	high	1898:1901	high	1898:1901	The genetic relationships between cheese-making property traits and milk composition were moderate to high.					
30197141	6	88	theme	environmental	1117:1129	arg1	effects					1145:1151	the random additive genetic, permanent environmental, and residual effects	1078:1151	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	1	89	theme	pressed	175:181	arg1	cheeses					190:196	pressed cooked cheeses	175:196	pressed cooked cheeses (PCC)	175:202	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	1	89	theme	pressed	175:181	arg1	PCC					199:201	PCC	199:201	PCC	199:201	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	13	90	dep	correlated	2090:2099	arg1	whereas					2170:2176	whereas	2170:2176	whereas	2170:2176	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	4	91	theme	MIR	727:729	arg1	spectra					731:737	1,100,238 MIR spectra	717:737	1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows	717:780	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	11	92	theme	property	1844:1851	arg1	traits					1853:1858	cheese-making property traits	1830:1858	cheese-making property traits	1830:1858	The genetic relationships between cheese-making property traits and milk composition were moderate to high.					
30197141	1	93	theme	cheeses	190:196	arg1	properties					161:170	Cheese-making properties	147:170	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC)	147:224	Cheese-making properties of pressed cooked cheeses (PCC) and soft cheeses (SC) were predicted from mid-infrared (MIR) spectra.					
30197141	9	94	theme	different	1515:1523	arg1	measures					1525:1532	different measures	1515:1532	different measures of cheese yield	1515:1548	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	6	95	theme	lactation	1028:1036	arg1	stage					1019:1023	stage	1019:1023	stage of lactation	1019:1036	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	6	95	theme	lactation	1028:1036	arg1	spectrometer					1005:1016	herd × test day × spectrometer	987:1016	herd × test day × spectrometer	987:1016	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	6	95	theme	lactation	1028:1036	arg1	month					1050:1054	year × month	1043:1054	year × month of calving	1043:1065	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	12	96	theme	proteins	1934:1941	arg1	levels					1924:1929	high levels	1919:1929	high levels of proteins, fatty acids, Ca, P, and Mg in milk	1919:1977	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30197141	13	97	theme	cheese	2236:2241	arg1	yields					2243:2248	cheese yields	2236:2248	cheese yields than with coagulation traits	2236:2277	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	13	98	from	Proteins	2047:2054	arg1	milk					2059:2062	milk	2059:2062	milk	2059:2062	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	14	99	theme	cheese	2373:2378	arg1	yields					2380:2385	cheese yields	2373:2385	cheese yields	2373:2385	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	2	100	theme	initial	484:490	arg1	pH					492:493	initial pH	484:493	initial pH; pH0PPC	484:501	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	11	101	theme	milk	1864:1867	arg1	composition					1869:1879	milk composition	1864:1879	milk composition	1864:1879	The genetic relationships between cheese-making property traits and milk composition were moderate to high.					
30197141	6	102	theme	stage	1019:1023	arg1	effects					1145:1151	the random additive genetic, permanent environmental, and residual effects	1078:1151	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	6	102	theme	stage	1019:1023	arg1	effects					976:982	the fixed effects	966:982	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	0	103	theme	cows	141:144	arg1	set					121:123	a large data set	108:123	a large data set of Montbéliarde cows	108:144	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	0	103	theme	cows	141:144	arg1	cows					141:144	Montbéliarde cows	128:144	Montbéliarde cows	128:144	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	3	104	theme	determination	521:533	arg1	Coefficients					505:516	Coefficients	505:516	Coefficients of determination of these traits	505:549	Coefficients of determination of these traits ranged between 0.54 and 0.89.					
30197141	12	105	from	Mg	1968:1969	arg1	milk					1974:1977	milk	1974:1977	milk	1974:1977	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30197141	9	106	theme	yield	1544:1548	arg1	measures					1525:1532	different measures	1515:1532	different measures of cheese yield	1515:1548	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	6	107	theme	random	1082:1087	arg1	effects					1145:1151	the random additive genetic, permanent environmental, and residual effects	1078:1151	the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects	966:1151	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	8	108	dep	0.39	1406:1409	arg1	to					1403:1404	to	1403:1404	to	1403:1404	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	15	109	theme	breeding	2585:2592	arg1	objectives					2594:2603	breeding objectives	2585:2603	breeding objectives without additional phenotyping cost	2585:2639	Cheese-making traits, as predicted from MIR spectra, are moderately heritable and could be integrated into breeding objectives without additional phenotyping cost, thus creating an opportunity for efficient improvement via selection.					
30197141	8	110	with	ones	1284:1287	arg1	heritability					1306:1317	the highest heritability	1294:1317	the highest heritability (0.42 to 0.48)	1294:1332	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	8	110	with	ones	1284:1287	arg1	0.48					1328:1331	0.48	1328:1331	0.48	1328:1331	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	6	111	theme	animal	930:935	arg1	model					937:941	A univariate or bivariate repeatability animal model	890:941	model	937:941	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	7	112	theme	cheese-making	1216:1228	arg1	traits					1239:1244	the 9 cheese-making property traits	1210:1244	the 9 cheese-making property traits analyzed	1210:1253	Heritability estimates varied between 0.37 and 0.48 for the 9 cheese-making property traits analyzed.					
30197141	4	113	theme	fatty	632:636	arg1	traits					624:629	milk composition traits	607:629	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content)	581:690	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	113	theme	fatty	632:636	arg1	acid					638:641	fatty acid	632:641	fatty acid	632:641	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	6	114	theme	bivariate	906:914	arg1	model					937:941	A univariate or bivariate repeatability animal model	890:941	model	937:941	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	4	115	from	cows	777:780	arg1	spectra					731:737	1,100,238 MIR spectra	717:737	1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows	717:780	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	5	116	theme	data	794:797	arg1	set					799:801	this data set	789:801	this data set	789:801	Using this data set, we estimated the corresponding genetic parameters of these traits by REML procedures.					
30197141	2	117	theme	coagulation	429:439	arg1	traits					441:446	5 coagulation traits	427:446	5 coagulation traits	427:446	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	8	118	theme	pH0	1361:1363	arg1	PPC					1365:1367	pH0 PPC	1361:1367	pH0 PPC	1361:1367	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	4	119	theme	milk	607:610	arg1	lactose					662:668	lactose	662:668	lactose	662:668	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	119	theme	milk	607:610	arg1	protein					644:650	protein	644:650	protein	644:650	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	119	theme	milk	607:610	arg1	content					683:689	citrate content	675:689	citrate content	675:689	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	119	theme	milk	607:610	arg1	traits					624:629	milk composition traits	607:629	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content)	581:690	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	119	theme	milk	607:610	arg1	acid					638:641	fatty acid	632:641	fatty acid	632:641	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	13	120	from	acids	2184:2188	arg1	milk					2193:2196	milk	2193:2196	milk	2193:2196	Proteins in milk were strongly genetically correlated with coagulation traits and, to a lesser extent, with cheese yields, whereas fatty acids in milk were more genetically correlated with cheese yields than with coagulation traits.					
30197141	12	121	theme	fatty	1944:1948	arg1	proteins					1934:1941	proteins	1934:1941	proteins	1934:1941	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30197141	12	121	theme	fatty	1944:1948	arg1	acids					1950:1954	fatty acids	1944:1954	fatty acids	1944:1954	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30197141	15	122	theme	additional	2613:2622	arg1	phenotyping					2624:2634	additional phenotyping	2613:2634	additional phenotyping cost	2613:2639	Cheese-making traits, as predicted from MIR spectra, are moderately heritable and could be integrated into breeding objectives without additional phenotyping cost, thus creating an opportunity for efficient improvement via selection.					
30197141	9	123	theme	coagulation	1605:1615	arg1	traits					1617:1622	coagulation traits	1605:1622	coagulation traits	1605:1622	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	0	124	theme	data	116:119	arg1	set					121:123	a large data set	108:123	a large data set of Montbéliarde cows	108:144	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	0	124	theme	data	116:119	arg1	cows					141:144	Montbéliarde cows	128:144	Montbéliarde cows	128:144	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	2	125	theme	1	453:453	arg1	acidification					455:467	acidification	455:467	acidification	455:467	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	8	126	theme	lowest	1377:1382	arg1	heritability					1384:1395	the lowest heritability	1373:1395	the lowest heritability (0.37 to 0.39)	1373:1410	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	8	126	theme	lowest	1377:1382	arg1	0.39					1406:1409	0.39	1406:1409	0.39	1406:1409	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	12	127	theme	better	2000:2005	arg1	yields					2014:2019	better cheese yields	2000:2019	better cheese yields	2000:2019	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30197141	5	128	theme	corresponding	821:833	arg1	parameters					843:852	the corresponding genetic parameters	817:852	the corresponding genetic parameters of these traits	817:868	Using this data set, we estimated the corresponding genetic parameters of these traits by REML procedures.					
30197141	14	129	theme	Montbéliarde	2324:2335	arg1	cows					2337:2340	Montbéliarde cows	2324:2340	Montbéliarde cows	2324:2340	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	0	130	theme	cheese-making	23:35	arg1	properties					37:46	cheese-making properties	23:46	cheese-making properties	23:46	Genetic parameters for cheese-making properties and milk composition predicted from mid-infrared spectra in a large data set of Montbéliarde cows.					
30197141	8	131	dep	0.48	1328:1331	arg1	to					1325:1326	to	1325:1326	to	1325:1326	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	9	132	theme	favorable	1420:1428	arg1	correlations					1438:1449	Strong favorable genetic correlations	1413:1449	Strong favorable genetic correlations	1413:1449	Strong favorable genetic correlations, with absolute values between 0.64 and 0.97, were found between different measures of cheese yield, between coagulation traits, between cheese yields and coagulation traits, and between coagulation traits measured for PCC and SC.					
30197141	8	133	theme	Coagulation	1256:1266	arg1	traits					1268:1273	Coagulation traits	1256:1273	Coagulation traits	1256:1273	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	8	133	theme	Coagulation	1256:1266	arg1	ones					1284:1287	the ones	1280:1287	the ones with the highest heritability (0.42 to 0.48)	1280:1332	Coagulation traits were the ones with the highest heritability (0.42 to 0.48), whereas cheese yields and pH0 PPC had the lowest heritability (0.37 to 0.39).					
30197141	2	134	theme	reference	359:367	arg1	measurements					369:380	reference measurements	359:380	reference measurements	359:380	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	2	135	theme	cheese	413:418	arg1	yield					420:424	laboratory cheese yield	402:424	laboratory cheese yield	402:424	The traits that were best predicted by MIR spectra (as determined by comparison with reference measurements) were 3 measures of laboratory cheese yield, 5 coagulation traits, and 1 acidification trait for PCC (initial pH; pH0PPC).					
30197141	6	136	theme	×	1003:1003	arg1	spectrometer					1005:1016	herd × test day × spectrometer	987:1016	herd × test day × spectrometer	987:1016	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	4	137	theme	citrate	675:681	arg1	content					683:689	citrate content	675:689	citrate content	675:689	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	4	137	theme	citrate	675:681	arg1	traits					624:629	milk composition traits	607:629	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content)	581:690	These 9 traits as well as milk composition traits (fatty acid, protein, mineral, lactose, and citrate content) were then predicted from 1,100,238 MIR spectra from 126,873 primiparous Montbéliarde cows.					
30197141	14	138	theme	properties	2408:2417	arg1	predictions					2358:2368	MIR predictions	2354:2368	MIR predictions of cheese yields and milk coagulation properties	2354:2417	This study, carried out on a large scale in Montbéliarde cows, shows that MIR predictions of cheese yields and milk coagulation properties are sufficiently accurate to be used for genetic analyses.					
30197141	12	139	from	P	1961:1961	arg1	milk					1974:1977	milk	1974:1977	milk	1974:1977	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30197141	6	140	theme	test	994:997	arg1	spectrometer					1005:1016	herd × test day × spectrometer	987:1016	herd × test day × spectrometer	987:1016	A univariate or bivariate repeatability animal model was used that included the fixed effects of herd × test day × spectrometer, stage of lactation, and year × month of calving as well as the random additive genetic, permanent environmental, and residual effects.					
30197141	12	141	from	Ca	1957:1958	arg1	milk					1974:1977	milk	1974:1977	milk	1974:1977	In particular, high levels of proteins, fatty acids, Ca, P, and Mg in milk were associated with better cheese yields and improved coagulation.					
30742170	9	0	theme	spectroscopy	1664:1675	arg1	tool					1691:1694	a powerful tool	1680:1694	a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs	1680:1849	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	9	0	theme	spectroscopy	1664:1675	arg1	use					1648:1650	the potential use	1634:1650	the potential use of ATR-FTIR spectroscopy	1634:1675	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	7	1	theme	pronounced	1122:1131	arg1	differences					1133:1143	pronounced differences	1122:1143	pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition	1122:1364	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	2	2	theme	tumor	286:290	arg1	relapse					292:298	tumor relapse	286:298	tumor relapse	286:298	The heterogeneous structure of the tumor mass includes cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis.					
30742170	5	3	theme	cellular	903:910	arg1	mechanisms					912:921	the cellular mechanisms	899:921	the cellular mechanisms	899:921	This work revealed the spectral differences in the cellular mechanisms and biochemical structures among three different cell types.					
30742170	4	4	theme	specific	832:839	arg1	therapies					841:849	the target specific therapies	821:849	the target specific therapies	821:849	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	5	theme	non-stem	660:667	arg1	non-CSCs					683:690	non-CSCs	683:690	non-CSCs	683:690	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	5	theme	non-stem	660:667	arg1	cells					676:680	non-stem cancer cells	660:680	non-stem cancer cells (non-CSCs)	660:691	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	1	6	theme	cancer	180:185	arg1	recurrence					166:175	the recurrence	162:175	the recurrence of cancer	162:185	Current cancer treatments destroy the tumor mass but cannot prevent the recurrence of cancer.					
30742170	4	7	theme	prostate	704:711	arg1	cells					724:728	normal prostate epithelial cells	697:728	normal prostate epithelial cells	697:728	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	2	8	theme	tumor	223:227	arg1	mass					229:232	the tumor mass	219:232	the tumor mass	219:232	The heterogeneous structure of the tumor mass includes cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis.					
30742170	1	9	theme	tumor	132:136	arg1	mass					138:141	the tumor mass	128:141	the tumor mass	128:141	Current cancer treatments destroy the tumor mass but cannot prevent the recurrence of cancer.					
30742170	5	10	theme	biochemical	927:937	arg1	structures					939:948	biochemical structures	927:948	biochemical structures	927:948	This work revealed the spectral differences in the cellular mechanisms and biochemical structures among three different cell types.					
30742170	9	11	theme	CSC	1749:1751	arg1	features					1753:1760	the CSC features	1745:1760	the CSC features	1745:1760	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	7	12	theme	carbohydrate	1341:1352	arg1	amount					1222:1227	protein amount	1214:1227	protein amount	1214:1227	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	12	theme	carbohydrate	1341:1352	arg1	composition					1354:1364	altered nucleic acid conformation and carbohydrate composition	1303:1364	composition	1354:1364	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	2	13	theme	stem	250:253	arg1	cells					255:259	cancer stem cells	243:259	cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis	243:345	The heterogeneous structure of the tumor mass includes cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis.					
30742170	4	14	theme	reflection-Fourier	497:514	arg1	spectroscopy					546:557	infrared (ATR-FTIR) spectroscopy	526:557	infrared (ATR-FTIR) spectroscopy	526:557	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	15	theme	infrared	526:533	arg1	spectroscopy					546:557	infrared (ATR-FTIR) spectroscopy	526:557	infrared (ATR-FTIR) spectroscopy	526:557	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	9	16	theme	new	1781:1783	arg1	strategies					1785:1794	new strategies	1781:1794	new strategies for cancer treatment	1781:1815	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	8	17	theme	prostate	1525:1532	arg1	CSCs					1534:1537	prostate CSCs	1525:1537	prostate CSCs in comparison to prostate non-CSCs and normal prostate cells	1525:1598	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	7	18	theme	protein	1214:1220	arg1	amount					1222:1227	protein amount	1214:1227	protein amount	1214:1227	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	18	theme	protein	1214:1220	arg1	abundance					1265:1273	the abundance	1261:1273	the abundance of nucleic acids (DNA/RNA)	1261:1300	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	18	theme	protein	1214:1220	arg1	conformation					1324:1335	altered nucleic acid conformation and carbohydrate composition	1303:1364	conformation	1324:1335	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	18	theme	protein	1214:1220	arg1	composition					1354:1364	altered nucleic acid conformation and carbohydrate composition	1303:1364	composition	1354:1364	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	0	19	theme	cancer	48:53	arg1	cells					60:64	CD133+/CD44+ human prostate cancer stem cells	20:64	CD133+/CD44+ human prostate cancer stem cells	20:64	Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy.					
30742170	6	20	theme	other	1087:1091	arg1	types					1098:1102	other cell types	1087:1102	other cell types	1087:1102	Particularly, prostate CSCs exhibit differences in the lipid composition and dynamics when compared to other cell types.					
30742170	4	21	theme	attenuated	480:489	arg1	spectroscopy					546:557	infrared (ATR-FTIR) spectroscopy	526:557	infrared (ATR-FTIR) spectroscopy	526:557	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	8	22	contain	containing	1397:1406	arg1	macromolecules					1382:1395	macromolecules	1382:1395	macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups	1382:1507	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	22	contain	containing	1397:1406	arg2	length					1427:1432	length	1427:1432	length	1427:1432	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	22	contain	containing	1397:1406	arg2	molecules					1476:1484	negatively charged molecules	1457:1484	negatively charged molecules	1457:1484	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	22	contain	containing	1397:1406	arg2	bond					1421:1424	the C[double bond	1408:1424	the C[double bond	1408:1424	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	22	contain	containing	1397:1406	arg2	groups					1446:1451	m-dash]O groups	1437:1451	m-dash]O groups	1437:1451	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	23	from	abundant	1513:1520	arg1	CSCs					1534:1537	prostate CSCs	1525:1537	prostate CSCs in comparison to prostate non-CSCs and normal prostate cells	1525:1598	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	1	24	theme	cancer	102:107	arg1	treatments					109:118	Current cancer treatments	94:118	Current cancer treatments	94:118	Current cancer treatments destroy the tumor mass but cannot prevent the recurrence of cancer.					
30742170	7	25	theme	acid	1319:1322	arg1	amount					1222:1227	protein amount	1214:1227	protein amount	1214:1227	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	25	theme	acid	1319:1322	arg1	conformation					1324:1335	altered nucleic acid conformation and carbohydrate composition	1303:1364	conformation	1324:1335	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	3	26	theme	cells	369:373	arg1	biology					352:358	The biology	348:358	The biology of these cells	348:373	The biology of these cells is still not fully understood; therefore, effective treatments cannot be developed sufficiently.					
30742170	4	27	theme	molecular	747:755	arg1	mechanisms					757:766	the molecular mechanisms	743:766	the molecular mechanisms	743:766	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	0	28	theme	CD133+/CD44+	20:31	arg1	cells					60:64	CD133+/CD44+ human prostate cancer stem cells	20:64	CD133+/CD44+ human prostate cancer stem cells	20:64	Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy.					
30742170	5	29	theme	different	962:970	arg1	types					977:981	three different cell types	956:981	three different cell types	956:981	This work revealed the spectral differences in the cellular mechanisms and biochemical structures among three different cell types.					
30742170	7	30	theme	altered	1303:1309	arg1	amount					1222:1227	protein amount	1214:1227	protein amount	1214:1227	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	30	theme	altered	1303:1309	arg1	conformation					1324:1335	altered nucleic acid conformation and carbohydrate composition	1303:1364	conformation	1324:1335	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	4	31	theme	unsupervised	574:585	arg1	analysis					600:607	unsupervised multivariate analysis	574:607	unsupervised multivariate analysis	574:607	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	0	32	theme	prostate	39:46	arg1	cells					60:64	CD133+/CD44+ human prostate cancer stem cells	20:64	CD133+/CD44+ human prostate cancer stem cells	20:64	Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy.					
30742170	8	33	theme	normal	1578:1583	arg1	cells					1594:1598	normal prostate cells	1578:1598	normal prostate cells	1578:1598	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	0	34	theme	stem	55:58	arg1	cells					60:64	CD133+/CD44+ human prostate cancer stem cells	20:64	CD133+/CD44+ human prostate cancer stem cells	20:64	Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy.					
30742170	4	35	theme	target	825:830	arg1	therapies					841:849	the target specific therapies	821:849	the target specific therapies	821:849	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	7	36	dep	amount	1222:1227	arg1	the					1210:1212	the	1210:1212	the	1210:1212	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	36	dep	amount	1222:1227	arg1	α-helices					1249:1257	α-helices	1249:1257	mainly α-helices	1242:1257	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	0	37	with	Characterization	0:15	arg1	spectroscopy					80:91	ATR-FTIR spectroscopy	71:91	ATR-FTIR spectroscopy	71:91	Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy.					
30742170	7	38	theme	nucleic	1278:1284	arg1	DNA/RNA					1293:1299	DNA/RNA	1293:1299	DNA/RNA	1293:1299	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	38	theme	nucleic	1278:1284	arg1	acids					1286:1290	nucleic acids	1278:1290	nucleic acids (DNA/RNA)	1278:1300	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	2	39	theme	heterogeneous	192:204	arg1	structure					206:214	The heterogeneous structure	188:214	The heterogeneous structure of the tumor mass	188:232	The heterogeneous structure of the tumor mass includes cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis.					
30742170	8	40	theme	O	1444:1444	arg1	groups					1446:1451	m-dash]O groups	1437:1451	m-dash]O groups	1437:1451	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	40	theme	O	1444:1444	arg1	bond					1421:1424	the C[double bond	1408:1424	the C[double bond	1408:1424	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	41	from	CSCs	1534:1537	arg1	abundant					1513:1520	abundant	1513:1520	abundant	1513:1520	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	41	from	CSCs	1534:1537	arg1	comparison					1542:1551	comparison	1542:1551	comparison to prostate non-CSCs and normal prostate cells	1542:1598	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	4	42	theme	ATR-FTIR	536:543	arg1	spectroscopy					546:557	infrared (ATR-FTIR) spectroscopy	526:557	infrared (ATR-FTIR) spectroscopy	526:557	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	9	43	theme	ATR-FTIR	1655:1662	arg1	spectroscopy					1664:1675	ATR-FTIR spectroscopy	1655:1675	ATR-FTIR spectroscopy	1655:1675	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	7	44	theme	major	1154:1158	arg1	macromolecules					1169:1182	their major cellular macromolecules	1148:1182	their major cellular macromolecules	1148:1182	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	44	theme	major	1154:1158	arg1	differences					1195:1205	differences	1195:1205	differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition	1195:1364	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	9	45	theme	powerful	1682:1689	arg1	tool					1691:1694	a powerful tool	1680:1694	a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs	1680:1849	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	9	45	theme	powerful	1682:1689	arg1	use					1648:1650	the potential use	1634:1650	the potential use of ATR-FTIR spectroscopy	1634:1675	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	4	46	theme	cancer	669:674	arg1	non-CSCs					683:690	non-CSCs	683:690	non-CSCs	683:690	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	46	theme	cancer	669:674	arg1	cells					676:680	non-stem cancer cells	660:680	non-stem cancer cells (non-CSCs)	660:691	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	2	47	theme	treatment	301:309	arg1	resistance					311:320	treatment resistance	301:320	treatment resistance	301:320	The heterogeneous structure of the tumor mass includes cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis.					
30742170	9	48	theme	new	1706:1708	arg1	insights					1710:1717	new insights	1706:1717	new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs	1706:1849	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	2	49	theme	cancer	243:248	arg1	cells					255:259	cancer stem cells	243:259	cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis	243:345	The heterogeneous structure of the tumor mass includes cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis.					
30742170	8	50	theme	charged	1468:1474	arg1	bond					1421:1424	the C[double bond	1408:1424	the C[double bond	1408:1424	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	50	theme	charged	1468:1474	arg1	molecules					1476:1484	negatively charged molecules	1457:1484	negatively charged molecules	1457:1484	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	2	51	theme	mass	229:232	arg1	structure					206:214	The heterogeneous structure	188:214	The heterogeneous structure of the tumor mass	188:232	The heterogeneous structure of the tumor mass includes cancer stem cells that are responsible for tumor relapse, treatment resistance, invasion and metastasis.					
30742170	8	52	dep	O	1444:1444	arg1	m-dash					1437:1442	m-dash	1437:1442	m-dash	1437:1442	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	4	53	theme	normal	697:702	arg1	cells					724:728	normal prostate epithelial cells	697:728	normal prostate epithelial cells	697:728	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	6	54	theme	lipid	1039:1043	arg1	composition					1045:1055	the lipid composition	1035:1055	the lipid composition	1035:1055	Particularly, prostate CSCs exhibit differences in the lipid composition and dynamics when compared to other cell types.					
30742170	8	55	theme	COO-	1497:1500	arg1	groups					1502:1507	the COO- groups	1493:1507	the COO- groups	1493:1507	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	4	56	theme	epithelial	713:722	arg1	cells					724:728	normal prostate epithelial cells	697:728	normal prostate epithelial cells	697:728	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	0	57	theme	ATR-FTIR	71:78	arg1	spectroscopy					80:91	ATR-FTIR spectroscopy	71:91	ATR-FTIR spectroscopy	71:91	Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy.					
30742170	5	58	theme	spectral	875:882	arg1	differences					884:894	the spectral differences	871:894	the spectral differences in the cellular mechanisms and biochemical structures among three different cell types	871:981	This work revealed the spectral differences in the cellular mechanisms and biochemical structures among three different cell types.					
30742170	9	59	theme	features	1753:1760	arg1	understanding					1728:1740	the understanding	1724:1740	the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs	1724:1849	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	4	60	dep	spectroscopy	546:557	arg1	transform					516:524	transform	516:524	transform	516:524	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	6	61	from	differences	1020:1030	arg1	dynamics					1061:1068	dynamics	1061:1068	dynamics	1061:1068	Particularly, prostate CSCs exhibit differences in the lipid composition and dynamics when compared to other cell types.					
30742170	6	61	from	differences	1020:1030	arg1	composition					1045:1055	the lipid composition	1035:1055	the lipid composition	1035:1055	Particularly, prostate CSCs exhibit differences in the lipid composition and dynamics when compared to other cell types.					
30742170	5	62	theme	cell	972:975	arg1	types					977:981	three different cell types	956:981	three different cell types	956:981	This work revealed the spectral differences in the cellular mechanisms and biochemical structures among three different cell types.					
30742170	6	63	theme	prostate	998:1005	arg1	CSCs					1007:1010	prostate CSCs	998:1010	prostate CSCs	998:1010	Particularly, prostate CSCs exhibit differences in the lipid composition and dynamics when compared to other cell types.					
30742170	5	64	from	differences	884:894	arg1	mechanisms					912:921	the cellular mechanisms	899:921	the cellular mechanisms	899:921	This work revealed the spectral differences in the cellular mechanisms and biochemical structures among three different cell types.					
30742170	5	64	from	differences	884:894	arg1	structures					939:948	biochemical structures	927:948	biochemical structures	927:948	This work revealed the spectral differences in the cellular mechanisms and biochemical structures among three different cell types.					
30742170	6	65	theme	cell	1093:1096	arg1	types					1098:1102	other cell types	1087:1102	other cell types	1087:1102	Particularly, prostate CSCs exhibit differences in the lipid composition and dynamics when compared to other cell types.					
30742170	4	66	theme	total	491:495	arg1	spectroscopy					546:557	infrared (ATR-FTIR) spectroscopy	526:557	infrared (ATR-FTIR) spectroscopy	526:557	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	1	67	theme	Current	94:100	arg1	treatments					109:118	Current cancer treatments	94:118	Current cancer treatments	94:118	Current cancer treatments destroy the tumor mass but cannot prevent the recurrence of cancer.					
30742170	7	68	theme	nucleic	1311:1317	arg1	amount					1222:1227	protein amount	1214:1227	protein amount	1214:1227	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	68	theme	nucleic	1311:1317	arg1	conformation					1324:1335	altered nucleic acid conformation and carbohydrate composition	1303:1364	conformation	1324:1335	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	9	69	theme	cancer	1800:1805	arg1	treatment					1807:1815	cancer treatment	1800:1815	cancer treatment	1800:1815	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	8	70	theme	prostate	1556:1563	arg1	non-CSCs					1565:1572	prostate non-CSCs	1556:1572	prostate non-CSCs	1556:1572	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	3	71	theme	effective	417:425	arg1	treatments					427:436	effective treatments	417:436	effective treatments	417:436	The biology of these cells is still not fully understood; therefore, effective treatments cannot be developed sufficiently.					
30742170	0	72	theme	human	33:37	arg1	cells					60:64	CD133+/CD44+ human prostate cancer stem cells	20:64	CD133+/CD44+ human prostate cancer stem cells	20:64	Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy.					
30742170	4	73	theme	multivariate	587:598	arg1	analysis					600:607	unsupervised multivariate analysis	574:607	unsupervised multivariate analysis	574:607	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	7	74	theme	cellular	1160:1167	arg1	macromolecules					1169:1182	their major cellular macromolecules	1148:1182	their major cellular macromolecules	1148:1182	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	74	theme	cellular	1160:1167	arg1	differences					1195:1205	differences	1195:1205	differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition	1195:1364	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	8	75	theme	prostate	1585:1592	arg1	cells					1594:1598	normal prostate cells	1578:1598	normal prostate cells	1578:1598	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	76	theme	C[double	1412:1419	arg1	groups					1446:1451	m-dash]O groups	1437:1451	m-dash]O groups	1437:1451	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	76	theme	C[double	1412:1419	arg1	length					1427:1432	length	1427:1432	length	1427:1432	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	76	theme	C[double	1412:1419	arg1	bond					1421:1424	the C[double bond	1408:1424	the C[double bond	1408:1424	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	76	theme	C[double	1412:1419	arg1	molecules					1476:1484	negatively charged molecules	1457:1484	negatively charged molecules	1457:1484	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	0	77	theme	cells	60:64	arg1	Characterization					0:15	Characterization	0:15	Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy.	0:92	Characterization of CD133+/CD44+ human prostate cancer stem cells with ATR-FTIR spectroscopy.					
30742170	7	78	from	differences	1195:1205	arg1	amount					1222:1227	protein amount	1214:1227	protein amount	1214:1227	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	78	from	differences	1195:1205	arg1	abundance					1265:1273	the abundance	1261:1273	the abundance of nucleic acids (DNA/RNA)	1261:1300	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	78	from	differences	1195:1205	arg1	conformation					1324:1335	altered nucleic acid conformation and carbohydrate composition	1303:1364	conformation	1324:1335	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	78	from	differences	1195:1205	arg1	composition					1354:1364	altered nucleic acid conformation and carbohydrate composition	1303:1364	composition	1354:1364	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	78	from	differences	1195:1205	arg1	content					1233:1239	content	1233:1239	content	1233:1239	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	79	theme	acids	1286:1290	arg1	amount					1222:1227	protein amount	1214:1227	protein amount	1214:1227	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	79	theme	acids	1286:1290	arg1	abundance					1265:1273	the abundance	1261:1273	the abundance of nucleic acids (DNA/RNA)	1261:1300	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	80	from	differences	1133:1143	arg1	macromolecules					1169:1182	their major cellular macromolecules	1148:1182	their major cellular macromolecules	1148:1182	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	7	80	from	differences	1133:1143	arg1	differences					1195:1205	differences	1195:1205	differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition	1195:1364	CSCs also harbor pronounced differences in their major cellular macromolecules, including differences in the protein amount and content (mainly α-helices), the abundance of nucleic acids (DNA/RNA), altered nucleic acid conformation and carbohydrate composition.					
30742170	4	81	theme	prostate	625:632	arg1	CSCs					653:656	CSCs	653:656	CSCs	653:656	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	81	theme	prostate	625:632	arg1	cells					646:650	prostate cancer stem cells	625:650	prostate cancer stem cells (CSCs)	625:657	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	82	theme	CSCs	784:787	arg1	features					772:779	features	772:779	features of CSCs, which are crucial to improving the target specific therapies	772:849	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	82	theme	CSCs	784:787	arg1	mechanisms					757:766	the molecular mechanisms	743:766	the molecular mechanisms	743:766	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	9	83	theme	potential	1638:1646	arg1	tool					1691:1694	a powerful tool	1680:1694	a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs	1680:1849	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	9	83	theme	potential	1638:1646	arg1	use					1648:1650	the potential use	1634:1650	the potential use of ATR-FTIR spectroscopy	1634:1675	Overall, this study demonstrates the potential use of ATR-FTIR spectroscopy as a powerful tool to obtain new insights into the understanding of the CSC features, which may provide new strategies for cancer treatment by selectively targeting the CSCs.					
30742170	8	84	contain	having	1486:1491	arg2	groups					1502:1507	the COO- groups	1493:1507	the COO- groups	1493:1507	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	84	contain	having	1486:1491	arg1	groups					1446:1451	m-dash]O groups	1437:1451	m-dash]O groups	1437:1451	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	8	84	contain	having	1486:1491	arg1	bond					1421:1424	the C[double bond	1408:1424	the C[double bond	1408:1424	Interestingly, macromolecules containing the C[double bond, length as m-dash]O groups and negatively charged molecules having the COO- groups are abundant in prostate CSCs in comparison to prostate non-CSCs and normal prostate cells.					
30742170	4	85	theme	stem	641:644	arg1	CSCs					653:656	CSCs	653:656	CSCs	653:656	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	85	theme	stem	641:644	arg1	cells					646:650	prostate cancer stem cells	625:650	prostate cancer stem cells (CSCs)	625:657	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	86	theme	cancer	634:639	arg1	CSCs					653:656	CSCs	653:656	CSCs	653:656	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30742170	4	86	theme	cancer	634:639	arg1	cells					646:650	prostate cancer stem cells	625:650	prostate cancer stem cells (CSCs)	625:657	Herein, attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, combined with unsupervised multivariate analysis, was applied to prostate cancer stem cells (CSCs), non-stem cancer cells (non-CSCs) and normal prostate epithelial cells to elucidate the molecular mechanisms and features of CSCs, which are crucial to improving the target specific therapies.					
30948056	13	0	theme	blank	1821:1825	arg1	control					1827:1833	the blank control	1817:1833	the blank control	1817:1833	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	3	1	theme	chitosan‑magnesium	488:505	arg1	membrane					515:522	a composite chitosan‑magnesium (CS-Mg) membrane	476:522	a composite chitosan‑magnesium (CS-Mg) membrane	476:522	In this study, a composite chitosan‑magnesium (CS-Mg) membrane was fabricated by dip-coating Mg alloy into chitosan solution.					
30948056	1	2	theme	excellent	240:248	arg1	biocompatibility					250:265	excellent biocompatibility	240:265	excellent biocompatibility	240:265	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	12	3	theme	samples	1658:1664	arg1	groups					1648:1653	Three groups	1642:1653	Three groups of samples	1642:1664	Three groups of samples were examined: CS-Mg, Heal-All, and a blank control.					
30948056	12	3	theme	samples	1658:1664	arg1	samples					1658:1664	samples	1658:1664	samples	1658:1664	Three groups of samples were examined: CS-Mg, Heal-All, and a blank control.					
30948056	14	4	theme	excellent	2044:2052	arg1	activity					2065:2072	excellent osteogenic activity	2044:2072	excellent osteogenic activity	2044:2072	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	11	5	theme	Rabbit	1562:1567	arg1	defects					1579:1585	Rabbit calvarial defects	1562:1585	Rabbit calvarial defects	1562:1585	Rabbit calvarial defects were used for testing the osteogenic activity in vivo.					
30948056	7	6	theme	alkaline	1084:1091	arg1	ALP					1106:1108	ALP	1106:1108	ALP	1106:1108	The in vitro biocompatibility was evaluated by cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells.					
30948056	7	6	theme	alkaline	1084:1091	arg1	phosphatase					1093:1103	alkaline phosphatase	1084:1103	alkaline phosphatase (ALP)	1084:1109	The in vitro biocompatibility was evaluated by cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells.					
30948056	10	7	theme	10	1374:1375	arg1	%					1376:1376	%	1376:1376	%	1376:1376	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	7	8	theme	cytotoxicity	1067:1078	arg1	assays					1111:1116	cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays	1052:1116	cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells	1052:1133	The in vitro biocompatibility was evaluated by cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells.					
30948056	1	9	theme	high	187:190	arg1	strength					192:199	high strength	187:199	high strength	187:199	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	14	10	theme	biodegradable	2011:2023	arg1	GBRM					2034:2037	a biodegradable metallic GBRM	2009:2037	a biodegradable metallic GBRM with excellent osteogenic activity	2009:2072	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	14	10	theme	biodegradable	2011:2023	arg1	potential					1980:1988	great potential	1974:1988	great potential for application	1974:2004	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	7	11	theme	adhesion	1057:1064	arg1	assays					1111:1116	cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays	1052:1116	cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells	1052:1133	The in vitro biocompatibility was evaluated by cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells.					
30948056	3	12	theme	dip-coating	542:552	arg1	alloy					557:561	dip-coating Mg alloy	542:561	dip-coating Mg alloy into chitosan solution	542:584	In this study, a composite chitosan‑magnesium (CS-Mg) membrane was fabricated by dip-coating Mg alloy into chitosan solution.					
30948056	8	13	theme	diluted	1188:1194	arg1	extracts					1196:1203	diluted extracts	1188:1203	diluted extracts of Mg, CS-Mg and Heal-All	1188:1229	The cytotoxicity and ALP assays were performed with diluted extracts of Mg, CS-Mg and Heal-All.					
30948056	10	14	theme	Mg	1538:1539	arg1	p > 0.05					1551:1558	p > 0.05	1551:1558	p > 0.05	1551:1558	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	10	14	theme	Mg	1538:1539	arg1	extracts					1541:1548	10% Mg extracts	1534:1548	10% Mg extracts (p > 0.05)	1534:1559	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	4	15	theme	biodegradable	683:695	arg1	membrane					657:664	this membrane	652:664	this membrane	652:664	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	4	15	theme	biodegradable	683:695	arg1	GBRM					697:700	biodegradable GBRM	683:700	biodegradable GBRM	683:700	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	10	16	theme	10	1534:1535	arg1	%					1536:1536	%	1536:1536	%	1536:1536	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	4	17	dep	in	600:601	arg1	vivo					603:606	vivo	603:606	vivo	603:606	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	3	18	theme	chitosan	568:575	arg1	solution					577:584	chitosan solution	568:584	chitosan solution	568:584	In this study, a composite chitosan‑magnesium (CS-Mg) membrane was fabricated by dip-coating Mg alloy into chitosan solution.					
30948056	1	19	theme	bone	138:141	arg1	GBRM					166:169	a guided bone regeneration membrane (GBRM)	129:170	a guided bone regeneration membrane (GBRM)	129:170	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	10	20	from	3	1426:1426	arg1	activity					1414:1421	higher ALP activity	1403:1421	higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts	1403:1507	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	0	21	theme	membrane	69:76	arg1	Biocompatibility					0:15	Biocompatibility	0:15	Biocompatibility	0:15	Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy.					
30948056	0	21	theme	membrane	69:76	arg1	activity					32:39	osteogenic activity	21:39	osteogenic activity	21:39	Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy.					
30948056	5	22	theme	dispersive	865:874	arg1	spectroscopy					876:887	scanning electron microscopy-electron dispersive spectroscopy	827:887	scanning electron microscopy-electron dispersive spectroscopy	827:887	The microstructure was analyzed by scanning electron microscopy-electron dispersive spectroscopy.					
30948056	10	23	from	5 days	1432:1437	arg1	activity					1414:1421	higher ALP activity	1403:1421	higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts	1403:1507	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	10	24	theme	CS-Mg	1378:1382	arg1	extracts					1384:1391	the 10% CS-Mg extracts	1370:1391	the 10% CS-Mg extracts	1370:1391	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	0	25	theme	magnesium	103:111	arg1	alloy					113:117	chitosan-coated magnesium alloy	87:117	chitosan-coated magnesium alloy	87:117	Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy.					
30948056	6	26	theme	modified	975:982	arg1	medium					990:995	Dulbecco's modified Eagle medium	964:995	Dulbecco's modified Eagle medium (DMEM)	964:1002	The degradation behavior was investigated by immersing the membranes into Dulbecco's modified Eagle medium (DMEM).					
30948056	6	26	theme	modified	975:982	arg1	DMEM					998:1001	DMEM	998:1001	DMEM	998:1001	The degradation behavior was investigated by immersing the membranes into Dulbecco's modified Eagle medium (DMEM).					
30948056	14	27	theme	CS-Mg	1953:1957	arg1	membrane					1959:1966	the CS-Mg membrane	1949:1966	the CS-Mg membrane	1949:1966	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	13	28	theme	bone	1741:1744	arg1	amounts					1726:1732	Higher amounts	1719:1732	Higher amounts of new bone	1719:1744	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	13	28	theme	bone	1741:1744	arg1	bone					1741:1744	new bone	1737:1744	new bone	1737:1744	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	9	29	theme	degradation	1275:1285	arg1	rate					1287:1290	a suitable degradation rate	1264:1290	a suitable degradation rate	1264:1290	The results show that CS-Mg has a suitable degradation rate, as well as similar cell adhesion and cytocompatibility to Heal-All.					
30948056	0	30	theme	guided	44:49	arg1	membrane					69:76	guided bone regeneration membrane	44:76	guided bone regeneration membrane	44:76	Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy.					
30948056	14	31	theme	osteogenic	2054:2063	arg1	activity					2065:2072	excellent osteogenic activity	2044:2072	excellent osteogenic activity	2044:2072	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	10	32	theme	ALP	1410:1412	arg1	activity					1414:1421	higher ALP activity	1403:1421	higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts	1403:1507	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	14	33	with	GBRM	2034:2037	arg1	activity					2065:2072	excellent osteogenic activity	2044:2072	excellent osteogenic activity	2044:2072	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	13	34	theme	Higher	1719:1724	arg1	amounts					1726:1732	Higher amounts	1719:1732	Higher amounts of new bone	1719:1744	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	13	34	theme	Higher	1719:1724	arg1	bone					1741:1744	new bone	1737:1744	new bone	1737:1744	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	2	35	theme	biomedical	405:414	arg1	field					416:420	the biomedical field	401:420	the biomedical field	401:420	Besides titanium, magnesium (Mg) is another metal widely used in the biomedical field, which also exhibits biodegradability.					
30948056	0	36	theme	osteogenic	21:30	arg1	activity					32:39	osteogenic activity	21:39	osteogenic activity	21:39	Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy.					
30948056	1	37	theme	regeneration	143:154	arg1	GBRM					166:169	a guided bone regeneration membrane (GBRM)	129:170	a guided bone regeneration membrane (GBRM)	129:170	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	1	38	contain	possess	179:185	arg2	strength					192:199	high strength	187:199	high strength	187:199	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	1	38	contain	possess	179:185	arg1	GBRM					166:169	a guided bone regeneration membrane (GBRM)	129:170	a guided bone regeneration membrane (GBRM)	129:170	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	9	39	theme	similar	1304:1310	arg1	adhesion					1317:1324	similar cell adhesion	1304:1324	similar cell adhesion	1304:1324	The results show that CS-Mg has a suitable degradation rate, as well as similar cell adhesion and cytocompatibility to Heal-All.					
30948056	1	40	theme	natural	297:303	arg1	membranes					325:333	natural absorbable collagen membranes	297:333	natural absorbable collagen membranes	297:333	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	13	41	theme	CS-Mg	1883:1887	arg1	groups					1902:1907	the CS-Mg and Heal-All groups	1879:1907	the CS-Mg and Heal-All groups	1879:1907	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	0	42	theme	bone	51:54	arg1	membrane					69:76	guided bone regeneration membrane	44:76	guided bone regeneration membrane	44:76	Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy.					
30948056	1	43	theme	collagen	316:323	arg1	membranes					325:333	natural absorbable collagen membranes	297:333	natural absorbable collagen membranes	297:333	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	13	44	theme	Heal-All	1893:1900	arg1	groups					1902:1907	the CS-Mg and Heal-All groups	1879:1907	the CS-Mg and Heal-All groups	1879:1907	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	1	45	theme	titanium	209:216	arg1	membranes					218:226	titanium membranes	209:226	titanium membranes	209:226	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	1	46	theme	membrane	156:163	arg1	GBRM					166:169	a guided bone regeneration membrane (GBRM)	129:170	a guided bone regeneration membrane (GBRM)	129:170	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	4	47	theme	In	587:588	arg1	tests					608:612	In vitro and in vivo tests	587:612	In vitro and in vivo tests	587:612	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	4	48	theme	commercial	764:773	arg1	Heal-All					781:788	Heal-All	781:788	Heal-All	781:788	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	4	48	theme	commercial	764:773	arg1	GBRM					775:778	a commercial GBRM	762:778	a commercial GBRM (Heal-All)	762:789	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	13	49	theme	significant	1847:1857	arg1	differences					1859:1869	no significant differences	1844:1869	no significant differences between the CS-Mg and Heal-All groups	1844:1907	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	11	50	used	used	1592:1595	arg2	defects					1579:1585	Rabbit calvarial defects	1562:1585	Rabbit calvarial defects	1562:1585	Rabbit calvarial defects were used for testing the osteogenic activity in vivo.					
30948056	9	51	theme	cell	1312:1315	arg1	adhesion					1317:1324	similar cell adhesion	1304:1324	similar cell adhesion	1304:1324	The results show that CS-Mg has a suitable degradation rate, as well as similar cell adhesion and cytocompatibility to Heal-All.					
30948056	10	52	theme	medium	1468:1473	arg1	control					1475:1481	the medium control	1464:1481	the medium control	1464:1481	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	3	53	theme	composite	478:486	arg1	membrane					515:522	a composite chitosan‑magnesium (CS-Mg) membrane	476:522	a composite chitosan‑magnesium (CS-Mg) membrane	476:522	In this study, a composite chitosan‑magnesium (CS-Mg) membrane was fabricated by dip-coating Mg alloy into chitosan solution.					
30948056	6	54	theme	degradation	894:904	arg1	behavior					906:913	The degradation behavior	890:913	The degradation behavior	890:913	The degradation behavior was investigated by immersing the membranes into Dulbecco's modified Eagle medium (DMEM).					
30948056	7	55	dep	in	1009:1010	arg1	vitro					1012:1016	vitro	1012:1016	vitro	1012:1016	The in vitro biocompatibility was evaluated by cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells.					
30948056	7	56	theme	phosphatase	1093:1103	arg1	assays					1111:1116	cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays	1052:1116	cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells	1052:1133	The in vitro biocompatibility was evaluated by cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells.					
30948056	10	57	theme	Heal-All	1491:1498	arg1	extracts					1500:1507	the Heal-All extracts	1487:1507	the Heal-All extracts	1487:1507	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	8	58	theme	cytotoxicity	1140:1151	arg1	assays					1161:1166	The cytotoxicity and ALP assays	1136:1166	assays	1161:1166	The cytotoxicity and ALP assays were performed with diluted extracts of Mg, CS-Mg and Heal-All.					
30948056	8	59	theme	Mg	1208:1209	arg1	extracts					1196:1203	diluted extracts	1188:1203	diluted extracts of Mg, CS-Mg and Heal-All	1188:1229	The cytotoxicity and ALP assays were performed with diluted extracts of Mg, CS-Mg and Heal-All.					
30948056	11	60	theme	calvarial	1569:1577	arg1	defects					1579:1585	Rabbit calvarial defects	1562:1585	Rabbit calvarial defects	1562:1585	Rabbit calvarial defects were used for testing the osteogenic activity in vivo.					
30948056	3	61	theme	Mg	554:555	arg1	alloy					557:561	dip-coating Mg alloy	542:561	dip-coating Mg alloy into chitosan solution	542:584	In this study, a composite chitosan‑magnesium (CS-Mg) membrane was fabricated by dip-coating Mg alloy into chitosan solution.					
30948056	5	62	theme	scanning	827:834	arg1	spectroscopy					876:887	scanning electron microscopy-electron dispersive spectroscopy	827:887	scanning electron microscopy-electron dispersive spectroscopy	827:887	The microstructure was analyzed by scanning electron microscopy-electron dispersive spectroscopy.					
30948056	8	63	theme	ALP	1157:1159	arg1	assays					1161:1166	The cytotoxicity and ALP assays	1136:1166	assays	1161:1166	The cytotoxicity and ALP assays were performed with diluted extracts of Mg, CS-Mg and Heal-All.					
30948056	8	64	theme	CS-Mg	1212:1216	arg1	extracts					1196:1203	diluted extracts	1188:1203	diluted extracts of Mg, CS-Mg and Heal-All	1188:1229	The cytotoxicity and ALP assays were performed with diluted extracts of Mg, CS-Mg and Heal-All.					
30948056	14	65	theme	metallic	2025:2032	arg1	GBRM					2034:2037	a biodegradable metallic GBRM	2009:2037	a biodegradable metallic GBRM with excellent osteogenic activity	2009:2072	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	14	65	theme	metallic	2025:2032	arg1	potential					1980:1988	great potential	1974:1988	great potential for application	1974:2004	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	7	66	theme	cell	1052:1055	arg1	adhesion					1057:1064	cell adhesion	1052:1064	cell adhesion	1052:1064	The in vitro biocompatibility was evaluated by cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells.					
30948056	8	67	theme	Heal-All	1222:1229	arg1	extracts					1196:1203	diluted extracts	1188:1203	diluted extracts of Mg, CS-Mg and Heal-All	1188:1229	The cytotoxicity and ALP assays were performed with diluted extracts of Mg, CS-Mg and Heal-All.					
30948056	5	68	theme	electron	836:843	arg1	spectroscopy					876:887	scanning electron microscopy-electron dispersive spectroscopy	827:887	scanning electron microscopy-electron dispersive spectroscopy	827:887	The microstructure was analyzed by scanning electron microscopy-electron dispersive spectroscopy.					
30948056	10	69	theme	%	1536:1536	arg1	p > 0.05					1551:1558	p > 0.05	1551:1558	p > 0.05	1551:1558	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	10	69	theme	%	1536:1536	arg1	extracts					1541:1548	10% Mg extracts	1534:1548	10% Mg extracts (p > 0.05)	1534:1559	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	13	70	theme	Heal-All	1776:1783	arg1	groups					1785:1790	the CS-Mg and Heal-All groups	1762:1790	the CS-Mg and Heal-All groups (p < 0.05)	1762:1801	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	13	70	theme	Heal-All	1776:1783	arg1	p < 0.05					1793:1800	p < 0.05	1793:1800	p < 0.05	1793:1800	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	7	71	theme	in	1009:1010	arg1	biocompatibility					1018:1033	The in vitro biocompatibility	1005:1033	The in vitro biocompatibility	1005:1033	The in vitro biocompatibility was evaluated by cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells.					
30948056	1	72	theme	guided	131:136	arg1	GBRM					166:169	a guided bone regeneration membrane (GBRM)	129:170	a guided bone regeneration membrane (GBRM)	129:170	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	13	73	theme	new	1737:1739	arg1	bone					1741:1744	new bone	1737:1744	new bone	1737:1744	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	4	74	dep	In	587:588	arg1	vitro					590:594	vitro	590:594	vitro	590:594	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	5	75	theme	microscopy-electron	845:863	arg1	spectroscopy					876:887	scanning electron microscopy-electron dispersive spectroscopy	827:887	scanning electron microscopy-electron dispersive spectroscopy	827:887	The microstructure was analyzed by scanning electron microscopy-electron dispersive spectroscopy.					
30948056	11	76	from	activity	1624:1631	arg1	vivo					1636:1639	vivo	1636:1639	vivo	1636:1639	Rabbit calvarial defects were used for testing the osteogenic activity in vivo.					
30948056	0	77	theme	chitosan-coated	87:101	arg1	alloy					113:117	chitosan-coated magnesium alloy	87:117	chitosan-coated magnesium alloy	87:117	Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy.					
30948056	14	78	theme	great	1974:1978	arg1	GBRM					2034:2037	a biodegradable metallic GBRM	2009:2037	a biodegradable metallic GBRM with excellent osteogenic activity	2009:2072	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	14	78	theme	great	1974:1978	arg1	potential					1980:1988	great potential	1974:1988	great potential for application	1974:2004	In conclusion, the CS-Mg membrane shows great potential for application as a biodegradable metallic GBRM with excellent osteogenic activity.					
30948056	13	79	theme	CS-Mg	1766:1770	arg1	groups					1785:1790	the CS-Mg and Heal-All groups	1762:1790	the CS-Mg and Heal-All groups (p < 0.05)	1762:1801	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	13	79	theme	CS-Mg	1766:1770	arg1	p < 0.05					1793:1800	p < 0.05	1793:1800	p < 0.05	1793:1800	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	6	80	theme	Eagle	984:988	arg1	medium					990:995	Dulbecco's modified Eagle medium	964:995	Dulbecco's modified Eagle medium (DMEM)	964:1002	The degradation behavior was investigated by immersing the membranes into Dulbecco's modified Eagle medium (DMEM).					
30948056	6	80	theme	Eagle	984:988	arg1	DMEM					998:1001	DMEM	998:1001	DMEM	998:1001	The degradation behavior was investigated by immersing the membranes into Dulbecco's modified Eagle medium (DMEM).					
30948056	10	81	theme	%	1376:1376	arg1	extracts					1384:1391	the 10% CS-Mg extracts	1370:1391	the 10% CS-Mg extracts	1370:1391	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	9	82	contain	has	1260:1262	arg1	CS-Mg					1254:1258	CS-Mg	1254:1258	CS-Mg	1254:1258	The results show that CS-Mg has a suitable degradation rate, as well as similar cell adhesion and cytocompatibility to Heal-All.					
30948056	9	82	contain	has	1260:1262	arg2	rate					1287:1290	a suitable degradation rate	1264:1290	a suitable degradation rate	1264:1290	The results show that CS-Mg has a suitable degradation rate, as well as similar cell adhesion and cytocompatibility to Heal-All.					
30948056	9	82	contain	has	1260:1262	arg2	adhesion					1317:1324	similar cell adhesion	1304:1324	similar cell adhesion	1304:1324	The results show that CS-Mg has a suitable degradation rate, as well as similar cell adhesion and cytocompatibility to Heal-All.					
30948056	9	83	theme	suitable	1266:1273	arg1	rate					1287:1290	a suitable degradation rate	1264:1290	a suitable degradation rate	1264:1290	The results show that CS-Mg has a suitable degradation rate, as well as similar cell adhesion and cytocompatibility to Heal-All.					
30948056	4	84	used	used	675:678	arg2	membrane					657:664	this membrane	652:664	this membrane	652:664	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	4	84	used	used	675:678	arg2	GBRM					697:700	biodegradable GBRM	683:700	biodegradable GBRM	683:700	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	10	85	theme	higher	1403:1408	arg1	activity					1414:1421	higher ALP activity	1403:1421	higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts	1403:1507	However, the 10% CS-Mg extracts exhibited higher ALP activity at 3 and 5 days (p < 0.05) compared with the medium control and the Heal-All extracts, but no differences with 10% Mg extracts (p > 0.05).					
30948056	4	86	theme	test	711:714	arg1	results					716:722	the test results	707:722	the test results	707:722	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	7	87	theme	MG63	1124:1127	arg1	cells					1129:1133	MG63 cells	1124:1133	MG63 cells	1124:1133	The in vitro biocompatibility was evaluated by cell adhesion, cytotoxicity and alkaline phosphatase (ALP) assays using MG63 cells.					
30948056	13	88	dep	observed	1914:1921	arg1	p > 0.1					1924:1930	p > 0.1	1924:1930	p > 0.1	1924:1930	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	0	89	theme	regeneration	56:67	arg1	membrane					69:76	guided bone regeneration membrane	44:76	guided bone regeneration membrane	44:76	Biocompatibility and osteogenic activity of guided bone regeneration membrane based on chitosan-coated magnesium alloy.					
30948056	1	90	theme	absorbable	305:314	arg1	membranes					325:333	natural absorbable collagen membranes	297:333	natural absorbable collagen membranes	297:333	Ideally, a guided bone regeneration membrane (GBRM) should possess high strength, as for titanium membranes, along with excellent biocompatibility and osteoconductivity, as for natural absorbable collagen membranes.					
30948056	13	91	dep	formed	1751:1756	arg1	whereas					1836:1842	whereas	1836:1842	whereas	1836:1842	Higher amounts of new bone were formed for the CS-Mg and Heal-All groups (p < 0.05) compared with the blank control, whereas no significant differences between the CS-Mg and Heal-All groups were observed (p > 0.1).					
30948056	12	92	theme	blank	1704:1708	arg1	control					1710:1716	a blank control	1702:1716	a blank control	1702:1716	Three groups of samples were examined: CS-Mg, Heal-All, and a blank control.					
30948056	11	93	theme	osteogenic	1613:1622	arg1	activity					1624:1631	the osteogenic activity	1609:1631	the osteogenic activity in vivo	1609:1639	Rabbit calvarial defects were used for testing the osteogenic activity in vivo.					
30948056	4	94	theme	in	600:601	arg1	tests					608:612	In vitro and in vivo tests	587:612	In vitro and in vivo tests	587:612	In vitro and in vivo tests were performed to investigate whether this membrane could be used as biodegradable GBRM, and the test results were compared with those obtained for a commercial GBRM (Heal-All).					
30948056	3	95	theme	CS-Mg	508:512	arg1	membrane					515:522	a composite chitosan‑magnesium (CS-Mg) membrane	476:522	a composite chitosan‑magnesium (CS-Mg) membrane	476:522	In this study, a composite chitosan‑magnesium (CS-Mg) membrane was fabricated by dip-coating Mg alloy into chitosan solution.					
29635707	3	0	theme	qualitative	439:449	arg1	aspects					451:457	quantitative and qualitative aspects	422:457	quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	422:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	5	1	dep	environment	880:890	arg1	°C					899:900	28/23 °C	893:900	28/23 °C	893:900	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	1	dep	environment	880:890	arg1	s-1					951:953	500 µmol m-2 s-1	938:953	500 µmol m-2 s-1	938:953	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	12	2	from	components	2358:2367	arg1	genotypes					2379:2387	lentil genotypes	2372:2387	lentil genotypes	2372:2387	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	3	3	from	aspects	451:457	arg1	genotypes					534:542	a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes	471:542	a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	471:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	3	4	theme	heat-tolerant	473:485	arg1	genotypes					534:542	a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes	471:542	a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	471:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	4	5	theme	%	719:719	arg1	humidity					730:737	60-65% relative humidity	714:737	60-65% relative humidity	714:737	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	9	6	dep	inhibited	1824:1832	arg1	%					1932:1932	22-42%	1927:1932	22-42%	1927:1932	Heat stress also inhibited the accumulation of storage proteins including albumins, globulins, prolamins and glutelins (22-42%).					
29635707	12	7	from	impacts	2402:2408	arg1	genotypes					2428:2436	heat-sensitive genotypes	2413:2436	heat-sensitive genotypes	2413:2436	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	1	8	theme	food	152:155	arg1	legume					157:162	a cool-season food legume	138:162	a cool-season food legume	138:162	BACKGROUND Lentil, a cool-season food legume, is highly sensitive to high temperatures, which drastically reduce biomass and seed yield.					
29635707	1	8	theme	food	152:155	arg1	Lentil					130:135	BACKGROUND Lentil	119:135	BACKGROUND Lentil	119:135	BACKGROUND Lentil, a cool-season food legume, is highly sensitive to high temperatures, which drastically reduce biomass and seed yield.					
29635707	10	9	theme	amino	1948:1952	arg1	acids					1954:1958	the amino acids	1944:1958	the amino acids	1944:1958	Most of the amino acids decreased significantly under heat stress in comparison to control, whereas some, such as proline, followed by glycine, alanine, isoleucine, leucine and lysine, increased.					
29635707	5	10	dep	stress	1038:1043	arg1	°C					1052:1053	33/28 °C	1046:1053	33/28 °C	1046:1053	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	9	11	theme	Heat	1807:1810	arg1	stress					1812:1817	Heat stress	1807:1817	Heat stress	1807:1817	Heat stress also inhibited the accumulation of storage proteins including albumins, globulins, prolamins and glutelins (22-42%).					
29635707	6	12	theme	growth	1238:1243	arg1	rate					1245:1248	the seed growth rate	1229:1248	the seed growth rate by 30-44%	1229:1258	Compared to control, heat stress reduced the seed growth rate by 30-44% and the seed-filling duration by 5.5-8.1 days, which ultimately reduced the seed yield by 38-58% and individual seed weights by 20-39%.					
29635707	8	13	theme	seed	1638:1641	arg1	reserves					1643:1650	seed reserves	1638:1650	seed reserves	1638:1650	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	5	14	theme	mean	906:909	arg1	day					911:913	mean day	906:913	mean day	906:913	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	6	15	theme	20-39	1388:1392	arg1	%					1393:1393	20-39%	1388:1393	20-39%	1388:1393	Compared to control, heat stress reduced the seed growth rate by 30-44% and the seed-filling duration by 5.5-8.1 days, which ultimately reduced the seed yield by 38-58% and individual seed weights by 20-39%.					
29635707	4	16	theme	%	778:778	arg1	flowering					780:788	50% flowering	776:788	50% flowering (114-115 days after sowing)	776:816	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	8	17	theme	heat	1664:1667	arg1	stress					1669:1674	heat stress	1664:1674	heat stress	1664:1674	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	5	18	theme	28/23	893:897	arg1	°C					899:900	28/23 °C	893:900	28/23 °C	893:900	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	18	theme	28/23	893:897	arg1	s-1					951:953	500 µmol m-2 s-1	938:953	500 µmol m-2 s-1	938:953	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	3	19	dep	heat-tolerant	473:485	arg1	HT					488:489	HT	488:489	HT; FLIP2009	488:499	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	0	20	from	yield	67:71	arg1	genotypes					108:116	lentil (Lens culinaris Medikus) genotypes	76:116	lentil (Lens culinaris Medikus) genotypes	76:116	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	5	21	theme	mean	1059:1062	arg1	day					1064:1066	as mean day	1056:1066	as mean day	1056:1066	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	9	22	theme	proteins	1862:1869	arg1	accumulation					1838:1849	the accumulation	1834:1849	the accumulation of storage proteins including albumins, globulins, prolamins and glutelins	1834:1924	Heat stress also inhibited the accumulation of storage proteins including albumins, globulins, prolamins and glutelins (22-42%).					
29635707	0	23	theme	seed	45:48	arg1	quality					50:56	seed quality	45:56	seed quality	45:56	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	5	24	theme	500	938:940	arg1	µmol					942:945	µmol	942:945	µmol	942:945	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	3	25	dep	HT	488:489	arg1	FLIP2009					492:499	FLIP2009	492:499	FLIP2009	492:499	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	12	26	theme	yield	2340:2344	arg1	components					2358:2367	seed yield and quality components	2335:2367	seed yield and quality components in lentil genotypes	2335:2387	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	5	27	theme	m-2	947:949	arg1	°C					899:900	28/23 °C	893:900	28/23 °C	893:900	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	27	theme	m-2	947:949	arg1	s-1					951:953	500 µmol m-2 s-1	938:953	500 µmol m-2 s-1	938:953	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	11	28	theme	Heat	2132:2135	arg1	stress					2137:2142	Heat stress	2132:2142	Heat stress	2132:2142	Heat stress reduced Ca (13-28%), Fe (17-52%), P (10-54%), K (12.4-28.3%) and Zn (36-59%) content in seeds, compared to the controls.					
29635707	12	29	theme	seed	2302:2305	arg1	filling					2307:2313	seed filling	2302:2313	seed filling	2302:2313	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	0	30	from	Impact	0:5	arg1	quality					50:56	seed quality	45:56	seed quality	45:56	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	0	30	from	Impact	0:5	arg1	yield					67:71	seed yield	62:71	seed yield	62:71	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	4	31	theme	mean	651:654	arg1	intensity					703:711	1350 µmol m-2 s-1 light intensity	679:711	1350 µmol m-2 s-1 light intensity	679:711	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	4	31	theme	mean	651:654	arg1	temperature					666:676	mean day/night temperature	651:676	mean day/night temperature	651:676	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	0	32	dep	lentil	76:81	arg1	Medikus					99:105	Lens culinaris Medikus	84:105	Lens culinaris Medikus	84:105	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	11	33	dep	Ca	2152:2153	arg1	content					2221:2227	content	2221:2227	content	2221:2227	Heat stress reduced Ca (13-28%), Fe (17-52%), P (10-54%), K (12.4-28.3%) and Zn (36-59%) content in seeds, compared to the controls.					
29635707	5	34	theme	500	1091:1093	arg1	µmol					1095:1098	µmol	1095:1098	µmol	1095:1098	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	0	35	theme	lentil	76:81	arg1	genotypes					108:116	lentil (Lens culinaris Medikus) genotypes	76:116	lentil (Lens culinaris Medikus) genotypes	76:116	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	8	36	theme	fat	1722:1724	arg1	content					1735:1741	protein (26-41%) and fat (39-57%) content	1701:1741	content	1735:1741	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	8	36	theme	fat	1722:1724	arg1	%					1732:1732	39-57%	1727:1732	39-57%	1727:1732	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	9	37	theme	storage	1854:1860	arg1	proteins					1862:1869	storage proteins	1854:1869	storage proteins	1854:1869	Heat stress also inhibited the accumulation of storage proteins including albumins, globulins, prolamins and glutelins (22-42%).					
29635707	3	38	dep	RESULTS	351:357	arg1	assessed					377:384	assessed	377:384	assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	377:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	5	39	theme	m-2	1100:1102	arg1	s-1					1104:1106	500 µmol m-2 s-1	1091:1106	500 µmol m-2 s-1	1091:1106	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	39	theme	m-2	1100:1102	arg1	humidity					1141:1148	60-65% relative humidity	1125:1148	60-65% relative humidity	1125:1148	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	40	theme	%	977:977	arg1	humidity					988:995	60-65% relative humidity	972:995	60-65% relative humidity;control	972:1003	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	6	41	theme	individual	1361:1370	arg1	weights					1377:1383	individual seed weights	1361:1383	individual seed weights	1361:1383	Compared to control, heat stress reduced the seed growth rate by 30-44% and the seed-filling duration by 5.5-8.1 days, which ultimately reduced the seed yield by 38-58% and individual seed weights by 20-39%.					
29635707	0	42	theme	heat	10:13	arg1	stress					15:20	heat stress	10:20	heat stress	10:20	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	1	43	theme	seed	244:247	arg1	yield					249:253	seed yield	244:253	seed yield	244:253	BACKGROUND Lentil, a cool-season food legume, is highly sensitive to high temperatures, which drastically reduce biomass and seed yield.					
29635707	5	44	dep	s-1	1104:1106	arg1	intensity					1114:1122	light intensity	1108:1122	light intensity	1108:1122	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	3	45	theme	controlled	549:558	arg1	environment					560:570	a controlled environment	547:570	a controlled environment	547:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	4	46	theme	light	697:701	arg1	intensity					703:711	1350 µmol m-2 s-1 light intensity	679:711	1350 µmol m-2 s-1 light intensity	679:711	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	4	46	theme	light	697:701	arg1	temperature					666:676	mean day/night temperature	651:676	mean day/night temperature	651:676	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	12	47	theme	heat-sensitive	2413:2426	arg1	genotypes					2428:2436	heat-sensitive genotypes	2413:2436	heat-sensitive genotypes	2413:2436	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	5	48	theme	light	955:959	arg1	intensity					961:969	light intensity	955:969	light intensity	955:969	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	7	49	theme	significant	1558:1568	arg1	reduction					1570:1578	a significant reduction	1556:1578	a significant reduction in relative leaf water content	1556:1609	Heat stress significantly damaged cell membranes and reduced chlorophyll concentration and fluorescence, and the photosynthetic rate, which was associated with a significant reduction in relative leaf water content.					
29635707	7	50	from	reduction	1570:1578	arg1	content					1603:1609	relative leaf water content	1583:1609	relative leaf water content	1583:1609	Heat stress significantly damaged cell membranes and reduced chlorophyll concentration and fluorescence, and the photosynthetic rate, which was associated with a significant reduction in relative leaf water content.					
29635707	5	51	theme	%	1130:1130	arg1	s-1					1104:1106	500 µmol m-2 s-1	1091:1106	500 µmol m-2 s-1	1091:1106	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	51	theme	%	1130:1130	arg1	humidity					1141:1148	60-65% relative humidity	1125:1148	60-65% relative humidity	1125:1148	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	2	52	theme	qualitative	286:296	arg1	aspects					315:321	qualitative and quantitative aspects	286:321	qualitative and quantitative aspects of seeds	286:330	The effects of heat stress on qualitative and quantitative aspects of seeds are not yet known.					
29635707	7	53	theme	water	1597:1601	arg1	content					1603:1609	relative leaf water content	1583:1609	relative leaf water content	1583:1609	Heat stress significantly damaged cell membranes and reduced chlorophyll concentration and fluorescence, and the photosynthetic rate, which was associated with a significant reduction in relative leaf water content.					
29635707	5	54	theme	controlled	869:878	arg1	environment					880:890	a controlled environment	867:890	a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control)	867:1004	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	2	55	theme	stress	276:281	arg1	effects					260:266	The effects	256:266	The effects of heat stress on qualitative and quantitative aspects of seeds	256:330	The effects of heat stress on qualitative and quantitative aspects of seeds are not yet known.					
29635707	7	56	theme	relative	1583:1590	arg1	content					1603:1609	relative leaf water content	1583:1609	relative leaf water content	1583:1609	Heat stress significantly damaged cell membranes and reduced chlorophyll concentration and fluorescence, and the photosynthetic rate, which was associated with a significant reduction in relative leaf water content.					
29635707	5	57	dep	day	911:913	arg1	temperature					925:935	temperature	925:935	temperature	925:935	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	8	58	theme	total	1758:1762	arg1	%					1777:1777	36-68%	1772:1777	36-68%	1772:1777	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	8	58	theme	total	1758:1762	arg1	sugars					1764:1769	total sugars	1758:1769	total sugars (36-68%)	1758:1778	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	4	59	theme	m-2	689:691	arg1	s-1					693:695	1350 µmol m-2 s-1	679:695	1350 µmol m-2 s-1 light intensity	679:711	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	5	60	theme	light	1108:1112	arg1	intensity					1114:1122	light intensity	1108:1122	light intensity	1108:1122	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	12	61	theme	quality	2350:2356	arg1	components					2358:2367	seed yield and quality components	2335:2367	seed yield and quality components in lentil genotypes	2335:2387	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	6	62	theme	heat	1209:1212	arg1	stress					1214:1219	heat stress	1209:1219	heat stress	1209:1219	Compared to control, heat stress reduced the seed growth rate by 30-44% and the seed-filling duration by 5.5-8.1 days, which ultimately reduced the seed yield by 38-58% and individual seed weights by 20-39%.					
29635707	1	63	theme	high	188:191	arg1	temperatures					193:204	high temperatures	188:204	high temperatures	188:204	BACKGROUND Lentil, a cool-season food legume, is highly sensitive to high temperatures, which drastically reduce biomass and seed yield.					
29635707	5	64	dep	day	1064:1066	arg1	temperature					1078:1088	temperature	1078:1088	temperature	1078:1088	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	2	65	theme	quantitative	302:313	arg1	aspects					315:321	qualitative and quantitative aspects	286:321	qualitative and quantitative aspects of seeds	286:330	The effects of heat stress on qualitative and quantitative aspects of seeds are not yet known.					
29635707	5	66	theme	µmol	942:945	arg1	°C					899:900	28/23 °C	893:900	28/23 °C	893:900	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	66	theme	µmol	942:945	arg1	s-1					951:953	500 µmol m-2 s-1	938:953	500 µmol m-2 s-1	938:953	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	12	67	dep	CONCLUSIONS	2265:2275	arg1	temperatures					2282:2293	High temperatures	2277:2293	CONCLUSIONS High temperatures during seed filling	2265:2313	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	7	68	theme	photosynthetic	1509:1522	arg1	rate					1524:1527	the photosynthetic rate	1505:1527	the photosynthetic rate	1505:1527	Heat stress significantly damaged cell membranes and reduced chlorophyll concentration and fluorescence, and the photosynthetic rate, which was associated with a significant reduction in relative leaf water content.					
29635707	5	69	dep	°C	899:900	arg1	humidity					988:995	60-65% relative humidity	972:995	60-65% relative humidity;control	972:1003	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	69	dep	°C	899:900	arg1	intensity					961:969	light intensity	955:969	light intensity	955:969	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	3	70	from	genotypes	534:542	arg1	aspects					451:457	quantitative and qualitative aspects	422:457	quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	422:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	3	70	from	genotypes	534:542	arg1	environment					560:570	a controlled environment	547:570	a controlled environment	547:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	5	71	dep	°C	1052:1053	arg1	night					1072:1076	night	1072:1076	night	1072:1076	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	71	dep	°C	1052:1053	arg1	day					1064:1066	as mean day	1056:1066	as mean day	1056:1066	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	71	dep	°C	1052:1053	arg1	s-1					1104:1106	500 µmol m-2 s-1	1091:1106	500 µmol m-2 s-1	1091:1106	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	71	dep	°C	1052:1053	arg1	humidity					1141:1148	60-65% relative humidity	1125:1148	60-65% relative humidity	1125:1148	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	7	72	theme	cell	1430:1433	arg1	membranes					1435:1443	cell membranes	1430:1443	cell membranes	1430:1443	Heat stress significantly damaged cell membranes and reduced chlorophyll concentration and fluorescence, and the photosynthetic rate, which was associated with a significant reduction in relative leaf water content.					
29635707	3	73	theme	seeds	462:466	arg1	aspects					451:457	quantitative and qualitative aspects	422:457	quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	422:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	1	74	theme	cool-season	140:150	arg1	legume					157:162	a cool-season food legume	138:162	a cool-season food legume	138:162	BACKGROUND Lentil, a cool-season food legume, is highly sensitive to high temperatures, which drastically reduce biomass and seed yield.					
29635707	1	74	theme	cool-season	140:150	arg1	Lentil					130:135	BACKGROUND Lentil	119:135	BACKGROUND Lentil	119:135	BACKGROUND Lentil, a cool-season food legume, is highly sensitive to high temperatures, which drastically reduce biomass and seed yield.					
29635707	12	75	theme	seed	2335:2338	arg1	yield					2340:2344	seed yield	2335:2344	seed yield	2335:2344	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	8	76	theme	proximate	1616:1624	arg1	analysis					1626:1633	The proximate analysis	1612:1633	The proximate analysis of seed reserves	1612:1650	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	3	77	dep	heat-sensitive	506:519	arg1	HS					522:523	HS	522:523	HS; IG4242	522:531	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	4	78	theme	relative	721:728	arg1	humidity					730:737	60-65% relative humidity	714:737	60-65% relative humidity	714:737	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	3	79	dep	HS	522:523	arg1	IG4242					526:531	IG4242	526:531	IG4242	526:531	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	3	80	from	effects	390:396	arg1	aspects					451:457	quantitative and qualitative aspects	422:457	quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	422:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	12	81	theme	lentil	2372:2377	arg1	genotypes					2379:2387	lentil genotypes	2372:2387	lentil genotypes	2372:2387	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	8	82	theme	reserves	1643:1650	arg1	analysis					1626:1633	The proximate analysis	1612:1633	The proximate analysis of seed reserves	1612:1650	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	3	83	theme	heat-sensitive	506:519	arg1	genotypes					534:542	a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes	471:542	a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	471:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	6	84	theme	seed	1233:1236	arg1	rate					1245:1248	the seed growth rate	1229:1248	the seed growth rate by 30-44%	1229:1258	Compared to control, heat stress reduced the seed growth rate by 30-44% and the seed-filling duration by 5.5-8.1 days, which ultimately reduced the seed yield by 38-58% and individual seed weights by 20-39%.					
29635707	0	85	from	quality	50:56	arg1	genotypes					108:116	lentil (Lens culinaris Medikus) genotypes	76:116	lentil (Lens culinaris Medikus) genotypes	76:116	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	8	86	dep	reduced	1676:1682	arg1	%					1697:1697	25-43%	1692:1697	25-43%	1692:1697	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	4	87	dep	22/10	642:646	arg1	humidity					730:737	60-65% relative humidity	714:737	60-65% relative humidity	714:737	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	4	87	dep	22/10	642:646	arg1	intensity					703:711	1350 µmol m-2 s-1 light intensity	679:711	1350 µmol m-2 s-1 light intensity	679:711	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	4	87	dep	22/10	642:646	arg1	temperature					666:676	mean day/night temperature	651:676	mean day/night temperature	651:676	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	7	88	theme	chlorophyll	1457:1467	arg1	concentration					1469:1481	chlorophyll concentration	1457:1481	chlorophyll concentration	1457:1481	Heat stress significantly damaged cell membranes and reduced chlorophyll concentration and fluorescence, and the photosynthetic rate, which was associated with a significant reduction in relative leaf water content.					
29635707	4	89	theme	50	776:777	arg1	%					778:778	%	778:778	%	778:778	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	6	90	theme	seed	1372:1375	arg1	weights					1377:1383	individual seed weights	1361:1383	individual seed weights	1361:1383	Compared to control, heat stress reduced the seed growth rate by 30-44% and the seed-filling duration by 5.5-8.1 days, which ultimately reduced the seed yield by 38-58% and individual seed weights by 20-39%.					
29635707	4	91	theme	natural	612:618	arg1	22/10					642:646	22/10	642:646	22/10	642:646	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	4	91	theme	natural	612:618	arg1	environment					629:639	a natural, outdoor environment	610:639	environment	629:639	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	5	92	theme	as	1056:1057	arg1	day					1064:1066	as mean day	1056:1066	as mean day	1056:1066	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	3	93	theme	high	401:404	arg1	temperatures					406:417	high temperatures	401:417	high temperatures	401:417	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	5	94	dep	humidity	988:995	arg1	control					997:1003	control	997:1003	60-65% relative humidity;control	972:1003	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	95	theme	heat	1033:1036	arg1	stress					1038:1043	heat stress	1033:1043	heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity)	1033:1149	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	0	96	theme	seed	62:65	arg1	yield					67:71	seed yield	62:71	seed yield	62:71	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	4	97	from	November	745:752	arg1	22/10					642:646	22/10	642:646	22/10	642:646	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	4	97	from	November	745:752	arg1	environment					629:639	a natural, outdoor environment	610:639	environment	629:639	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	6	98	theme	seed-filling	1268:1279	arg1	duration					1281:1288	the seed-filling duration	1264:1288	the seed-filling duration by 5.5-8.1 days, which ultimately reduced the seed yield by 38-58% and individual seed weights by 20-39%	1264:1393	Compared to control, heat stress reduced the seed growth rate by 30-44% and the seed-filling duration by 5.5-8.1 days, which ultimately reduced the seed yield by 38-58% and individual seed weights by 20-39%.					
29635707	1	99	theme	BACKGROUND	119:128	arg1	legume					157:162	a cool-season food legume	138:162	a cool-season food legume	138:162	BACKGROUND Lentil, a cool-season food legume, is highly sensitive to high temperatures, which drastically reduce biomass and seed yield.					
29635707	1	99	theme	BACKGROUND	119:128	arg1	Lentil					130:135	BACKGROUND Lentil	119:135	BACKGROUND Lentil	119:135	BACKGROUND Lentil, a cool-season food legume, is highly sensitive to high temperatures, which drastically reduce biomass and seed yield.					
29635707	3	100	from	seeds	462:466	arg1	genotypes					534:542	a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes	471:542	a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	471:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	10	101	theme	heat	1990:1993	arg1	stress					1995:2000	heat stress	1990:2000	heat stress	1990:2000	Most of the amino acids decreased significantly under heat stress in comparison to control, whereas some, such as proline, followed by glycine, alanine, isoleucine, leucine and lysine, increased.					
29635707	2	102	from	effects	260:266	arg1	aspects					315:321	qualitative and quantitative aspects	286:321	qualitative and quantitative aspects of seeds	286:330	The effects of heat stress on qualitative and quantitative aspects of seeds are not yet known.					
29635707	13	103	theme	Chemical	2457:2464	arg1	Industry					2466:2473	Chemical Industry	2457:2473	Chemical Industry	2457:2473	© 2018 Society of Chemical Industry.					
29635707	2	104	theme	heat	271:274	arg1	stress					276:281	heat stress	271:281	heat stress	271:281	The effects of heat stress on qualitative and quantitative aspects of seeds are not yet known.					
29635707	7	105	theme	Heat	1396:1399	arg1	stress					1401:1406	Heat stress	1396:1406	Heat stress	1396:1406	Heat stress significantly damaged cell membranes and reduced chlorophyll concentration and fluorescence, and the photosynthetic rate, which was associated with a significant reduction in relative leaf water content.					
29635707	4	106	theme	day/night	656:664	arg1	intensity					703:711	1350 µmol m-2 s-1 light intensity	679:711	1350 µmol m-2 s-1 light intensity	679:711	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	4	106	theme	day/night	656:664	arg1	temperature					666:676	mean day/night temperature	651:676	mean day/night temperature	651:676	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	5	107	theme	µmol	1095:1098	arg1	s-1					1104:1106	500 µmol m-2 s-1	1091:1106	500 µmol m-2 s-1	1091:1106	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	107	theme	µmol	1095:1098	arg1	humidity					1141:1148	60-65% relative humidity	1125:1148	60-65% relative humidity	1125:1148	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	6	108	theme	seed	1336:1339	arg1	yield					1341:1345	the seed yield	1332:1345	the seed yield	1332:1345	Compared to control, heat stress reduced the seed growth rate by 30-44% and the seed-filling duration by 5.5-8.1 days, which ultimately reduced the seed yield by 38-58% and individual seed weights by 20-39%.					
29635707	4	109	theme	outdoor	621:627	arg1	22/10					642:646	22/10	642:646	22/10	642:646	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	4	109	theme	outdoor	621:627	arg1	environment					629:639	a natural, outdoor environment	610:639	environment	629:639	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	2	110	theme	seeds	326:330	arg1	aspects					315:321	qualitative and quantitative aspects	286:321	qualitative and quantitative aspects of seeds	286:330	The effects of heat stress on qualitative and quantitative aspects of seeds are not yet known.					
29635707	0	111	theme	stress	15:20	arg1	Impact					0:5	Impact	0:5	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.	0:117	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	5	112	theme	relative	979:986	arg1	humidity					988:995	60-65% relative humidity	972:995	60-65% relative humidity;control	972:1003	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	10	113	dep	control	2019:2025	arg1	comparison					2005:2014	comparison	2005:2014	comparison	2005:2014	Most of the amino acids decreased significantly under heat stress in comparison to control, whereas some, such as proline, followed by glycine, alanine, isoleucine, leucine and lysine, increased.					
29635707	12	114	theme	High	2277:2280	arg1	temperatures					2282:2293	High temperatures	2277:2293	CONCLUSIONS High temperatures during seed filling	2265:2313	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	12	115	theme	severe	2395:2400	arg1	impacts					2402:2408	severe impacts	2395:2408	severe impacts on heat-sensitive genotypes	2395:2436	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	0	116	theme	seed	29:32	arg1	filling					34:40	seed filling	29:40	seed filling	29:40	Impact of heat stress during seed filling on seed quality and seed yield in lentil (Lens culinaris Medikus) genotypes.					
29635707	4	117	theme	s-1	693:695	arg1	intensity					703:711	1350 µmol m-2 s-1 light intensity	679:711	1350 µmol m-2 s-1 light intensity	679:711	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	4	117	theme	s-1	693:695	arg1	temperature					666:676	mean day/night temperature	651:676	mean day/night temperature	651:676	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	5	118	theme	60-65	1125:1129	arg1	%					1130:1130	%	1130:1130	%	1130:1130	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	4	119	dep	flowering	780:788	arg1	days					799:802	114-115 days	791:802	114-115 days after sowing	791:815	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
29635707	7	120	theme	leaf	1592:1595	arg1	content					1603:1609	relative leaf water content	1583:1609	relative leaf water content	1583:1609	Heat stress significantly damaged cell membranes and reduced chlorophyll concentration and fluorescence, and the photosynthetic rate, which was associated with a significant reduction in relative leaf water content.					
29635707	12	121	with	detrimental	2319:2329	arg1	impacts					2402:2408	severe impacts	2395:2408	severe impacts on heat-sensitive genotypes	2395:2436	CONCLUSIONS High temperatures during seed filling are detrimental for seed yield and quality components in lentil genotypes, with severe impacts on heat-sensitive genotypes.					
29635707	5	122	theme	relative	1132:1139	arg1	s-1					1104:1106	500 µmol m-2 s-1	1091:1106	500 µmol m-2 s-1	1091:1106	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	122	theme	relative	1132:1139	arg1	humidity					1141:1148	60-65% relative humidity	1125:1148	60-65% relative humidity	1125:1148	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	3	123	theme	quantitative	422:433	arg1	aspects					451:457	quantitative and qualitative aspects	422:457	quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	422:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	5	124	theme	60-65	972:976	arg1	%					977:977	%	977:977	%	977:977	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	3	125	theme	temperatures	406:417	arg1	effects					390:396	the effects	386:396	the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment	386:570	RESULTS In this study, we assessed the effects of high temperatures on quantitative and qualitative aspects of seeds in a heat-tolerant (HT; FLIP2009) and heat-sensitive (HS; IG4242) genotypes in a controlled environment.					
29635707	10	126	dep	decreased	1960:1968	arg1	whereas					2028:2034	whereas	2028:2034	whereas	2028:2034	Most of the amino acids decreased significantly under heat stress in comparison to control, whereas some, such as proline, followed by glycine, alanine, isoleucine, leucine and lysine, increased.					
29635707	5	127	theme	plants	842:847	arg1	plants					842:847	plants	842:847	plants	842:847	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	5	127	theme	plants	842:847	arg1	set					835:837	one set	831:837	one set of plants	831:847	After that, one set of plants was maintained in a controlled environment (28/23 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity;control) and one set was exposed to heat stress (33/28 °C, as mean day and night temperature, 500 µmol m-2 s-1 light intensity, 60-65% relative humidity), where they remained until maturity.					
29635707	8	128	theme	relative	1781:1788	arg1	%					1777:1777	36-68%	1772:1777	36-68%	1772:1777	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	8	128	theme	relative	1781:1788	arg1	sugars					1764:1769	total sugars	1758:1769	total sugars (36-68%)	1758:1778	The proximate analysis of seed reserves showed that heat stress reduced starch (25-43%), protein (26-41%) and fat (39-57%) content, and increased total sugars (36-68%), relative to the controls.					
29635707	4	129	theme	µmol	684:687	arg1	s-1					693:695	1350 µmol m-2 s-1	679:695	1350 µmol m-2 s-1 light intensity	679:711	Initially, the plants were raised in a natural, outdoor environment (22/10 °C mean day/night temperature, 1350 µmol m-2 s-1 light intensity, 60-65% relative humidity) from November to mid-February until 50% flowering (114-115 days after sowing).					
30599455	0	0	theme	oil	79:81	arg1	combination					54:64	the combination	50:64	the combination of essential oil with carboxymethylcellulose	50:109	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.					
30599455	6	1	theme	cell	932:935	arg1	wall					937:940	the cell wall	928:940	the cell wall	928:940	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	3	2	from	activity	436:443	arg1	morphology					473:482	pathogen morphology	464:482	pathogen morphology	464:482	The chemical composition of the EO with the highest activity and its effects on pathogen morphology were verified.					
30599455	2	3	theme	plant	348:352	arg1	stolonifer					372:381	plant pathogen Rhizopus stolonifer	348:381	plant pathogen Rhizopus stolonifer	348:381	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	7	4	theme	L.	1018:1019	arg1	EO					1030:1031	L. sidoides EO	1018:1031	L. sidoides EO associated with CMC	1018:1051	Strawberries treated with L. sidoides EO associated with CMC presented a reduction in disease severity, especially when treated in a curative way.					
30599455	4	5	theme	CMC	582:584	arg1	coating					587:593	carboxymethylcellulose (CMC) coating	558:593	carboxymethylcellulose (CMC) coating	558:593	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications, was also evaluated.					
30599455	0	6	theme	essential	69:77	arg1	oil					79:81	essential oil	69:81	essential oil with carboxymethylcellulose	69:109	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.					
30599455	6	7	theme	thymol	812:817	arg1	prevalence					785:794	a prevalence	783:794	a prevalence of the compound thymol	783:817	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	0	8	with	oil	79:81	arg1	carboxymethylcellulose					88:109	carboxymethylcellulose	88:109	carboxymethylcellulose	88:109	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.					
30599455	4	9	from	coating	587:593	arg1	applications					623:634	preventive and curative applications	599:634	preventive and curative applications	599:634	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications, was also evaluated.					
30599455	0	10	from	Control	0:6	arg1	strawberries					34:45	strawberries	34:45	strawberries	34:45	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.					
30599455	7	11	theme	disease	1078:1084	arg1	severity					1086:1093	disease severity	1078:1093	disease severity	1078:1093	Strawberries treated with L. sidoides EO associated with CMC presented a reduction in disease severity, especially when treated in a curative way.					
30599455	3	12	with	composition	397:407	arg1	activity					436:443	the highest activity	424:443	the highest activity	424:443	The chemical composition of the EO with the highest activity and its effects on pathogen morphology were verified.					
30599455	3	12	with	composition	397:407	arg1	effects					453:459	its effects	449:459	its effects on pathogen morphology	449:482	The chemical composition of the EO with the highest activity and its effects on pathogen morphology were verified.					
30599455	6	13	theme	intracellular	950:962	arg1	components					964:973	the intracellular components	946:973	the intracellular components of the pathogen	946:989	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	6	14	theme	L.	885:886	arg1	EO					897:898	L. sidoides EO	885:898	L. sidoides EO	885:898	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	5	15	theme	L.	657:658	arg1	EO					669:670	L. sidoides EO	657:670	L. sidoides EO	657:670	L. sidoides EO presented the highest in vitro antifungal activity.					
30599455	4	16	theme	curative	614:621	arg1	applications					623:634	preventive and curative applications	599:634	preventive and curative applications	599:634	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications, was also evaluated.					
30599455	2	17	theme	staigeriana	260:270	arg1	EOs					241:243	EOs	241:243	EOs	241:243	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	2	17	theme	staigeriana	260:270	arg1	oils					235:238	the essential oils	221:238	the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus	221:318	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	3	18	theme	highest	428:434	arg1	activity					436:443	the highest activity	424:443	the highest activity	424:443	The chemical composition of the EO with the highest activity and its effects on pathogen morphology were verified.					
30599455	0	19	from	strawberries	34:45	arg1	Control					0:6	Control	0:6	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.	0:110	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.					
30599455	5	20	dep	in	694:695	arg1	vitro					697:701	vitro	697:701	vitro	697:701	L. sidoides EO presented the highest in vitro antifungal activity.					
30599455	6	21	theme	EO	773:774	arg1	composition					753:763	the chemical composition	740:763	the chemical composition of this EO	740:774	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	2	22	theme	pseudocaryophyllus	301:318	arg1	EOs					241:243	EOs	241:243	EOs	241:243	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	2	22	theme	pseudocaryophyllus	301:318	arg1	oils					235:238	the essential oils	221:238	the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus	221:318	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	7	23	theme	sidoides	1021:1028	arg1	EO					1030:1031	L. sidoides EO	1018:1031	L. sidoides EO associated with CMC	1018:1051	Strawberries treated with L. sidoides EO associated with CMC presented a reduction in disease severity, especially when treated in a curative way.					
30599455	1	24	theme	soft	184:187	arg1	rot					189:191	soft rot	184:191	soft rot	184:191	Strawberry has a limiting postharvest shelf life, especially because of soft rot.					
30599455	3	25	theme	chemical	388:395	arg1	composition					397:407	The chemical composition	384:407	The chemical composition of the EO with the highest activity and its effects on pathogen morphology	384:482	The chemical composition of the EO with the highest activity and its effects on pathogen morphology were verified.					
30599455	0	26	theme	stolonifer	20:29	arg1	Control					0:6	Control	0:6	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.	0:110	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.					
30599455	4	27	theme	EO	539:540	arg1	activity					522:529	The in vivo antifungal activity	499:529	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications,	499:635	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications, was also evaluated.					
30599455	4	28	dep	in	503:504	arg1	vivo					506:509	vivo	506:509	vivo	506:509	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications, was also evaluated.					
30599455	0	29	theme	Rhizopus	11:18	arg1	stolonifer					20:29	Rhizopus stolonifer	11:29	Rhizopus stolonifer in strawberries	11:45	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.					
30599455	4	30	theme	carboxymethylcellulose	558:579	arg1	coating					587:593	carboxymethylcellulose (CMC) coating	558:593	carboxymethylcellulose (CMC) coating	558:593	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications, was also evaluated.					
30599455	5	31	theme	in	694:695	arg1	activity					714:721	in vitro antifungal activity	694:721	the highest in vitro antifungal activity	682:721	L. sidoides EO presented the highest in vitro antifungal activity.					
30599455	6	32	theme	electron	853:860	arg1	microscopy					862:871	the scanning and transmission electron microscopy	823:871	microscopy	862:871	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	2	33	theme	sidoides	280:287	arg1	EOs					241:243	EOs	241:243	EOs	241:243	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	2	33	theme	sidoides	280:287	arg1	oils					235:238	the essential oils	221:238	the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus	221:318	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	1	34	theme	shelf	150:154	arg1	life					156:159	a limiting postharvest shelf life	127:159	a limiting postharvest shelf life	127:159	Strawberry has a limiting postharvest shelf life, especially because of soft rot.					
30599455	6	35	theme	transmission	840:851	arg1	microscopy					862:871	the scanning and transmission electron microscopy	823:871	microscopy	862:871	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	2	36	theme	oils	235:238	arg1	activity					209:216	The antifungal activity	194:216	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus	194:318	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	3	37	theme	EO	416:417	arg1	composition					397:407	The chemical composition	384:407	The chemical composition of the EO with the highest activity and its effects on pathogen morphology	384:482	The chemical composition of the EO with the highest activity and its effects on pathogen morphology were verified.					
30599455	5	38	theme	antifungal	703:712	arg1	activity					714:721	in vitro antifungal activity	694:721	the highest in vitro antifungal activity	682:721	L. sidoides EO presented the highest in vitro antifungal activity.					
30599455	2	39	theme	essential	225:233	arg1	EOs					241:243	EOs	241:243	EOs	241:243	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	2	39	theme	essential	225:233	arg1	oils					235:238	the essential oils	221:238	the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus	221:318	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	6	40	theme	composition	753:763	arg1	analysis					728:735	The analysis	724:735	The analysis of the chemical composition of this EO	724:774	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	7	41	from	reduction	1065:1073	arg1	severity					1086:1093	disease severity	1078:1093	disease severity	1078:1093	Strawberries treated with L. sidoides EO associated with CMC presented a reduction in disease severity, especially when treated in a curative way.					
30599455	4	42	theme	antifungal	511:520	arg1	activity					522:529	The in vivo antifungal activity	499:529	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications,	499:635	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications, was also evaluated.					
30599455	5	43	theme	sidoides	660:667	arg1	EO					669:670	L. sidoides EO	657:670	L. sidoides EO	657:670	L. sidoides EO presented the highest in vitro antifungal activity.					
30599455	6	44	theme	chemical	744:751	arg1	composition					753:763	the chemical composition	740:763	the chemical composition of this EO	740:774	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	1	45	contain	has	123:125	arg2	life					156:159	a limiting postharvest shelf life	127:159	a limiting postharvest shelf life	127:159	Strawberry has a limiting postharvest shelf life, especially because of soft rot.					
30599455	1	45	contain	has	123:125	arg1	Strawberry					112:121	Strawberry	112:121	Strawberry	112:121	Strawberry has a limiting postharvest shelf life, especially because of soft rot.					
30599455	5	46	theme	highest	686:692	arg1	activity					714:721	in vitro antifungal activity	694:721	the highest in vitro antifungal activity	682:721	L. sidoides EO presented the highest in vitro antifungal activity.					
30599455	3	47	from	effects	453:459	arg1	morphology					473:482	pathogen morphology	464:482	pathogen morphology	464:482	The chemical composition of the EO with the highest activity and its effects on pathogen morphology were verified.					
30599455	6	48	theme	pathogen	982:989	arg1	damage					918:923	damage	918:923	damage to the cell wall	918:940	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	6	48	theme	pathogen	982:989	arg1	components					964:973	the intracellular components	946:973	the intracellular components of the pathogen	946:989	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	7	49	theme	curative	1125:1132	arg1	way					1134:1136	a curative way	1123:1136	a curative way	1123:1136	Strawberries treated with L. sidoides EO associated with CMC presented a reduction in disease severity, especially when treated in a curative way.					
30599455	2	50	theme	antifungal	198:207	arg1	activity					209:216	The antifungal activity	194:216	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus	194:318	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	1	51	theme	limiting	129:136	arg1	life					156:159	a limiting postharvest shelf life	127:159	a limiting postharvest shelf life	127:159	Strawberry has a limiting postharvest shelf life, especially because of soft rot.					
30599455	4	52	theme	in	503:504	arg1	activity					522:529	The in vivo antifungal activity	499:529	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications,	499:635	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications, was also evaluated.					
30599455	1	53	theme	postharvest	138:148	arg1	life					156:159	a limiting postharvest shelf life	127:159	a limiting postharvest shelf life	127:159	Strawberry has a limiting postharvest shelf life, especially because of soft rot.					
30599455	3	54	theme	pathogen	464:471	arg1	morphology					473:482	pathogen morphology	464:482	pathogen morphology	464:482	The chemical composition of the EO with the highest activity and its effects on pathogen morphology were verified.					
30599455	6	55	theme	compound	803:810	arg1	thymol					812:817	the compound thymol	799:817	the compound thymol	799:817	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	2	56	theme	Rhizopus	363:370	arg1	stolonifer					372:381	plant pathogen Rhizopus stolonifer	348:381	plant pathogen Rhizopus stolonifer	348:381	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	6	57	theme	sidoides	888:895	arg1	EO					897:898	L. sidoides EO	885:898	L. sidoides EO	885:898	The analysis of the chemical composition of this EO showed a prevalence of the compound thymol and the scanning and transmission electron microscopy showed that L. sidoides EO was able to cause damage to the cell wall and the intracellular components of the pathogen.					
30599455	0	58	from	stolonifer	20:29	arg1	strawberries					34:45	strawberries	34:45	strawberries	34:45	Control of Rhizopus stolonifer in strawberries by the combination of essential oil with carboxymethylcellulose.					
30599455	2	59	theme	pathogen	354:361	arg1	stolonifer					372:381	plant pathogen Rhizopus stolonifer	348:381	plant pathogen Rhizopus stolonifer	348:381	The antifungal activity of the essential oils (EOs) of Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus was tested in vitro against plant pathogen Rhizopus stolonifer.					
30599455	4	60	theme	preventive	599:608	arg1	applications					623:634	preventive and curative applications	599:634	preventive and curative applications	599:634	The in vivo antifungal activity of this EO associated with carboxymethylcellulose (CMC) coating, in preventive and curative applications, was also evaluated.					
29253227	2	0	from	impact	363:368	arg1	function					391:398	function	391:398	function	391:398	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	2	0	from	impact	363:368	arg1	structure					377:385	structure	377:385	structure	377:385	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	6	1	theme	operational	1124:1134	arg1	OTU					1153:1155	OTU	1153:1155	OTU	1153:1155	In the ilea, 2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium were enriched (P = 0.016 and P = 0.007, respectively) in the avilamycin-fed group, and an OTU belonging to Lactobacillus crispatus was decreased (P = 0.016).					
29253227	6	1	theme	operational	1124:1134	arg1	units					1146:1150	2 operational taxonomic units	1122:1150	2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium	1122:1207	In the ilea, 2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium were enriched (P = 0.016 and P = 0.007, respectively) in the avilamycin-fed group, and an OTU belonging to Lactobacillus crispatus was decreased (P = 0.016).					
29253227	3	2	from	chickens	564:571	arg1	communities					518:528	the bacterial communities	504:528	the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet	504:612	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	3	3	theme	rRNA	662:665	arg1	genes					667:671	bacterial 16S rRNA genes	648:671	bacterial 16S rRNA genes	648:671	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	8	4	theme	communities	1584:1594	arg1	profiling					1561:1569	Functional profiling	1550:1569	Functional profiling of bacterial communities based on PICRUSt analysis	1550:1620	Functional profiling of bacterial communities based on PICRUSt analysis revealed that 10 functional categories were enriched by avilamycin treatments, and 4 functional categories were decreased.					
29253227	3	5	theme	bacterial	648:656	arg1	genes					667:671	bacterial 16S rRNA genes	648:671	bacterial 16S rRNA genes	648:671	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	9	6	theme	supplementation	1818:1832	arg1	influence					1794:1802	the influence	1790:1802	the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota	1790:1915	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	9	6	theme	supplementation	1818:1832	arg1	different					1931:1939	different	1931:1939	different	1931:1939	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	1	7	theme	feed	163:166	arg1	efficiencies					168:179	feed efficiencies	163:179	feed efficiencies	163:179	The mechanisms by which antibiotic growth promoters (AGP) enhance growth rates, feed efficiencies, and disease resistance in poultry need to be understood for designing safer and alternative strategies to replace AGP.					
29253227	7	8	theme	avilamycin	1510:1519	arg1	P					1532:1532	P = 0.005-0.047	1532:1546	P = 0.005-0.047	1532:1546	In the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU, 12 OTU were decreased, and 3 were increased in response to avilamycin treatment (P = 0.005-0.047).					
29253227	7	8	theme	avilamycin	1510:1519	arg1	treatment					1521:1529	avilamycin treatment	1510:1529	avilamycin treatment (P = 0.005-0.047)	1510:1547	In the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU, 12 OTU were decreased, and 3 were increased in response to avilamycin treatment (P = 0.005-0.047).					
29253227	5	9	theme	bacterial	1016:1024	arg1	communities					1026:1036	the cecal bacterial communities	1006:1036	the cecal bacterial communities	1006:1036	Multivariate analyses revealed that the ileal microbiota of the avilamycin-fed group were clearly distinguished from those of the control group, whereas the cecal bacterial communities were apparently not influenced by feeding diets containing avilamycin.					
29253227	8	10	theme	functional	1639:1648	arg1	categories					1650:1659	10 functional categories	1636:1659	10 functional categories	1636:1659	Functional profiling of bacterial communities based on PICRUSt analysis revealed that 10 functional categories were enriched by avilamycin treatments, and 4 functional categories were decreased.					
29253227	1	11	theme	disease	186:192	arg1	resistance					194:203	disease resistance	186:203	disease resistance	186:203	The mechanisms by which antibiotic growth promoters (AGP) enhance growth rates, feed efficiencies, and disease resistance in poultry need to be understood for designing safer and alternative strategies to replace AGP.					
29253227	0	12	from	microbiota	51:60	arg1	chickens					73:80	broiler chickens	65:80	broiler chickens	65:80	Influence of dietary avilamycin on ileal and cecal microbiota in broiler chickens.					
29253227	10	13	theme	commensal	2061:2069	arg1	bacteria					2071:2078	commensal bacteria	2061:2078	commensal bacteria	2061:2078	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	5	14	theme	control	983:989	arg1	group					991:995	the control group	979:995	the control group	979:995	Multivariate analyses revealed that the ileal microbiota of the avilamycin-fed group were clearly distinguished from those of the control group, whereas the cecal bacterial communities were apparently not influenced by feeding diets containing avilamycin.					
29253227	1	15	theme	antibiotic	107:116	arg1	AGP					136:138	AGP	136:138	AGP	136:138	The mechanisms by which antibiotic growth promoters (AGP) enhance growth rates, feed efficiencies, and disease resistance in poultry need to be understood for designing safer and alternative strategies to replace AGP.					
29253227	1	15	theme	antibiotic	107:116	arg1	promoters					125:133	antibiotic growth promoters	107:133	antibiotic growth promoters (AGP)	107:139	The mechanisms by which antibiotic growth promoters (AGP) enhance growth rates, feed efficiencies, and disease resistance in poultry need to be understood for designing safer and alternative strategies to replace AGP.					
29253227	9	16	dep	diversity	1841:1849	arg1	the					1837:1839	the	1837:1839	the	1837:1839	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	5	17	theme	Multivariate	853:864	arg1	analyses					866:873	Multivariate analyses	853:873	Multivariate analyses	853:873	Multivariate analyses revealed that the ileal microbiota of the avilamycin-fed group were clearly distinguished from those of the control group, whereas the cecal bacterial communities were apparently not influenced by feeding diets containing avilamycin.					
29253227	5	18	contain	containing	1086:1095	arg2	avilamycin					1097:1106	avilamycin	1097:1106	avilamycin	1097:1106	Multivariate analyses revealed that the ileal microbiota of the avilamycin-fed group were clearly distinguished from those of the control group, whereas the cecal bacterial communities were apparently not influenced by feeding diets containing avilamycin.					
29253227	5	18	contain	containing	1086:1095	arg1	diets					1080:1084	diets	1080:1084	diets containing avilamycin	1080:1106	Multivariate analyses revealed that the ileal microbiota of the avilamycin-fed group were clearly distinguished from those of the control group, whereas the cecal bacterial communities were apparently not influenced by feeding diets containing avilamycin.					
29253227	9	19	from	influence	1794:1802	arg1	profiles					1890:1897	functional profiles	1879:1897	functional profiles	1879:1897	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	9	19	from	influence	1794:1802	arg1	diversity					1841:1849	diversity	1841:1849	diversity	1841:1849	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	9	19	from	influence	1794:1802	arg1	composition					1862:1872	taxonomic composition	1852:1872	taxonomic composition	1852:1872	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	10	20	theme	chicken	2087:2093	arg1	tract					2112:2116	the chicken gastrointestinal tract	2083:2116	the chicken gastrointestinal tract	2083:2116	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	2	21	theme	broiler	425:431	arg1	chickens					433:440	broiler chickens	425:440	broiler chickens	425:440	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	6	22	dep	Lactobacillus	1171:1183	arg1	reuteri					1185:1191	reuteri	1185:1191	reuteri	1185:1191	In the ilea, 2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium were enriched (P = 0.016 and P = 0.007, respectively) in the avilamycin-fed group, and an OTU belonging to Lactobacillus crispatus was decreased (P = 0.016).					
29253227	0	23	theme	broiler	65:71	arg1	chickens					73:80	broiler chickens	65:80	broiler chickens	65:80	Influence of dietary avilamycin on ileal and cecal microbiota in broiler chickens.					
29253227	7	24	dep	treatment	1521:1529	arg1	response					1498:1505	response	1498:1505	response	1498:1505	In the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU, 12 OTU were decreased, and 3 were increased in response to avilamycin treatment (P = 0.005-0.047).					
29253227	0	25	from	Influence	0:8	arg1	microbiota					51:60	ileal and cecal microbiota	35:60	ileal and cecal microbiota in broiler chickens	35:80	Influence of dietary avilamycin on ileal and cecal microbiota in broiler chickens.					
29253227	2	26	used	used	328:331	arg2	AGP					339:341	an AGP	336:341	an AGP in poultry	336:352	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	2	26	used	used	328:331	arg2	Avilamycin					301:310	Avilamycin	301:310	Avilamycin	301:310	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	10	27	from	understanding	1991:2003	arg1	activity					2049:2056	activity	2049:2056	activity	2049:2056	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	10	27	from	understanding	1991:2003	arg1	composition					2033:2043	composition	2033:2043	composition	2033:2043	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	6	28	theme	avilamycin-fed	1270:1283	arg1	group					1285:1289	the avilamycin-fed group	1266:1289	the avilamycin-fed group	1266:1289	In the ilea, 2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium were enriched (P = 0.016 and P = 0.007, respectively) in the avilamycin-fed group, and an OTU belonging to Lactobacillus crispatus was decreased (P = 0.016).					
29253227	8	29	theme	functional	1707:1716	arg1	categories					1718:1727	4 functional categories	1705:1727	4 functional categories	1705:1727	Functional profiling of bacterial communities based on PICRUSt analysis revealed that 10 functional categories were enriched by avilamycin treatments, and 4 functional categories were decreased.					
29253227	4	30	theme	ileal	717:721	arg1	higher					747:752	higher	747:752	higher	747:752	Alpha diversity metrics indicated that the ileal bacterial diversity was higher in avilamycin-fed chickens than in the control group, whereas the opposite was true for the cecum.					
29253227	4	30	theme	ileal	717:721	arg1	diversity					733:741	the ileal bacterial diversity	713:741	the ileal bacterial diversity	713:741	Alpha diversity metrics indicated that the ileal bacterial diversity was higher in avilamycin-fed chickens than in the control group, whereas the opposite was true for the cecum.					
29253227	5	31	theme	avilamycin-fed	917:930	arg1	group					932:936	the avilamycin-fed group	913:936	the avilamycin-fed group	913:936	Multivariate analyses revealed that the ileal microbiota of the avilamycin-fed group were clearly distinguished from those of the control group, whereas the cecal bacterial communities were apparently not influenced by feeding diets containing avilamycin.					
29253227	4	32	theme	Alpha	674:678	arg1	metrics					690:696	Alpha diversity metrics	674:696	Alpha diversity metrics	674:696	Alpha diversity metrics indicated that the ileal bacterial diversity was higher in avilamycin-fed chickens than in the control group, whereas the opposite was true for the cecum.					
29253227	10	33	theme	feeding	2135:2141	arg1	strategies					2143:2152	novel feeding strategies	2129:2152	novel feeding strategies for improving animal health and performance	2129:2196	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	3	34	from	communities	518:528	arg1	chickens					564:571	broiler chickens	556:571	broiler chickens fed with an avilamycin-supplemented diet	556:612	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	0	35	theme	dietary	13:19	arg1	avilamycin					21:30	dietary avilamycin	13:30	dietary avilamycin	13:30	Influence of dietary avilamycin on ileal and cecal microbiota in broiler chickens.					
29253227	7	36	theme	non-singleton	1432:1444	arg1	OTU					1446:1448	1,286 non-singleton OTU	1426:1448	1,286 non-singleton OTU	1426:1448	In the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU, 12 OTU were decreased, and 3 were increased in response to avilamycin treatment (P = 0.005-0.047).					
29253227	5	37	theme	ileal	893:897	arg1	microbiota					899:908	the ileal microbiota	889:908	the ileal microbiota of the avilamycin-fed group	889:936	Multivariate analyses revealed that the ileal microbiota of the avilamycin-fed group were clearly distinguished from those of the control group, whereas the cecal bacterial communities were apparently not influenced by feeding diets containing avilamycin.					
29253227	2	38	theme	microbiome	411:420	arg1	function					391:398	function	391:398	function	391:398	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	2	38	theme	microbiome	411:420	arg1	structure					377:385	structure	377:385	structure	377:385	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	4	39	theme	avilamycin-fed	757:770	arg1	chickens					772:779	avilamycin-fed chickens	757:779	avilamycin-fed chickens	757:779	Alpha diversity metrics indicated that the ileal bacterial diversity was higher in avilamycin-fed chickens than in the control group, whereas the opposite was true for the cecum.					
29253227	7	40	theme	higher	1404:1409	arg1	diversity					1411:1419	much higher diversity	1399:1419	much higher diversity	1399:1419	In the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU, 12 OTU were decreased, and 3 were increased in response to avilamycin treatment (P = 0.005-0.047).					
29253227	9	41	theme	avilamycin	1807:1816	arg1	supplementation					1818:1832	avilamycin supplementation	1807:1832	avilamycin supplementation	1807:1832	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	3	42	theme	avilamycin-supplemented	585:607	arg1	diet					609:612	an avilamycin-supplemented diet	582:612	an avilamycin-supplemented diet	582:612	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	7	43	theme	cecal	1374:1378	arg1	microbiota					1380:1389	the cecal microbiota	1370:1389	the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU	1370:1448	In the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU, 12 OTU were decreased, and 3 were increased in response to avilamycin treatment (P = 0.005-0.047).					
29253227	9	44	from	different	1931:1939	arg1	cecum					1958:1962	cecum	1958:1962	cecum	1958:1962	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	9	44	from	different	1931:1939	arg1	ileum					1948:1952	ileum	1948:1952	ileum	1948:1952	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	10	45	theme	gastrointestinal	2095:2110	arg1	tract					2112:2116	the chicken gastrointestinal tract	2083:2116	the chicken gastrointestinal tract	2083:2116	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	10	46	from	impact	2012:2017	arg1	activity					2049:2056	activity	2049:2056	activity	2049:2056	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	10	46	from	impact	2012:2017	arg1	composition					2033:2043	composition	2033:2043	composition	2033:2043	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	3	47	theme	high-throughput	618:632	arg1	sequencing					634:643	high-throughput sequencing	618:643	high-throughput sequencing of bacterial 16S rRNA genes	618:671	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	6	48	theme	=	1357:1357	arg1	P					1355:1355	P = 0.016	1355:1363	P = 0.016	1355:1363	In the ilea, 2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium were enriched (P = 0.016 and P = 0.007, respectively) in the avilamycin-fed group, and an OTU belonging to Lactobacillus crispatus was decreased (P = 0.016).					
29253227	10	49	theme	animal	2168:2173	arg1	health					2175:2180	animal health	2168:2180	animal health	2168:2180	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	10	50	theme	AGP	2022:2024	arg1	impact					2012:2017	the impact	2008:2017	the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract	2008:2116	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	3	51	theme	genes	667:671	arg1	sequencing					634:643	high-throughput sequencing	618:643	high-throughput sequencing of bacterial 16S rRNA genes	618:671	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	8	52	theme	bacterial	1574:1582	arg1	communities					1584:1594	bacterial communities	1574:1594	bacterial communities based on PICRUSt analysis	1574:1620	Functional profiling of bacterial communities based on PICRUSt analysis revealed that 10 functional categories were enriched by avilamycin treatments, and 4 functional categories were decreased.					
29253227	1	53	theme	growth	149:154	arg1	rates					156:160	growth rates	149:160	growth rates	149:160	The mechanisms by which antibiotic growth promoters (AGP) enhance growth rates, feed efficiencies, and disease resistance in poultry need to be understood for designing safer and alternative strategies to replace AGP.					
29253227	10	54	theme	impact	2012:2017	arg1	understanding					1991:2003	our understanding	1987:2003	our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance	1987:2196	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	10	55	from	activity	2049:2056	arg1	tract					2112:2116	the chicken gastrointestinal tract	2083:2116	the chicken gastrointestinal tract	2083:2116	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	10	55	from	activity	2049:2056	arg1	understanding					1991:2003	our understanding	1987:2003	our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance	1987:2196	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	3	56	theme	16S	658:660	arg1	genes					667:671	bacterial 16S rRNA genes	648:671	bacterial 16S rRNA genes	648:671	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	4	57	theme	control	793:799	arg1	group					801:805	the control group	789:805	the control group	789:805	Alpha diversity metrics indicated that the ileal bacterial diversity was higher in avilamycin-fed chickens than in the control group, whereas the opposite was true for the cecum.					
29253227	10	58	theme	novel	2129:2133	arg1	strategies					2143:2152	novel feeding strategies	2129:2152	novel feeding strategies for improving animal health and performance	2129:2196	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	3	59	dep	ileum	537:541	arg1	the					533:535	the	533:535	the	533:535	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	5	60	theme	cecal	1010:1014	arg1	communities					1026:1036	the cecal bacterial communities	1006:1036	the cecal bacterial communities	1006:1036	Multivariate analyses revealed that the ileal microbiota of the avilamycin-fed group were clearly distinguished from those of the control group, whereas the cecal bacterial communities were apparently not influenced by feeding diets containing avilamycin.					
29253227	10	61	theme	bacteria	2071:2078	arg1	activity					2049:2056	activity	2049:2056	activity	2049:2056	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	10	61	theme	bacteria	2071:2078	arg1	composition					2033:2043	composition	2033:2043	composition	2033:2043	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	3	62	theme	bacterial	508:516	arg1	communities					518:528	the bacterial communities	504:528	the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet	504:612	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	3	63	theme	cecum	547:551	arg1	communities					518:528	the bacterial communities	504:528	the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet	504:612	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	1	64	theme	growth	118:123	arg1	AGP					136:138	AGP	136:138	AGP	136:138	The mechanisms by which antibiotic growth promoters (AGP) enhance growth rates, feed efficiencies, and disease resistance in poultry need to be understood for designing safer and alternative strategies to replace AGP.					
29253227	1	64	theme	growth	118:123	arg1	promoters					125:133	antibiotic growth promoters	107:133	antibiotic growth promoters (AGP)	107:139	The mechanisms by which antibiotic growth promoters (AGP) enhance growth rates, feed efficiencies, and disease resistance in poultry need to be understood for designing safer and alternative strategies to replace AGP.					
29253227	9	65	theme	functional	1879:1888	arg1	profiles					1890:1897	functional profiles	1879:1897	functional profiles	1879:1897	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	8	66	theme	PICRUSt	1605:1611	arg1	analysis					1613:1620	PICRUSt analysis	1605:1620	PICRUSt analysis	1605:1620	Functional profiling of bacterial communities based on PICRUSt analysis revealed that 10 functional categories were enriched by avilamycin treatments, and 4 functional categories were decreased.					
29253227	3	67	theme	ileum	537:541	arg1	communities					518:528	the bacterial communities	504:528	the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet	504:612	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	2	68	theme	chickens	433:440	arg1	microbiome					411:420	the gut microbiome	403:420	the gut microbiome of broiler chickens	403:440	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	6	69	theme	taxonomic	1136:1144	arg1	OTU					1153:1155	OTU	1153:1155	OTU	1153:1155	In the ilea, 2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium were enriched (P = 0.016 and P = 0.007, respectively) in the avilamycin-fed group, and an OTU belonging to Lactobacillus crispatus was decreased (P = 0.016).					
29253227	6	69	theme	taxonomic	1136:1144	arg1	units					1146:1150	2 operational taxonomic units	1122:1150	2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium	1122:1207	In the ilea, 2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium were enriched (P = 0.016 and P = 0.007, respectively) in the avilamycin-fed group, and an OTU belonging to Lactobacillus crispatus was decreased (P = 0.016).					
29253227	8	70	theme	avilamycin	1678:1687	arg1	treatments					1689:1698	avilamycin treatments	1678:1698	avilamycin treatments	1678:1698	Functional profiling of bacterial communities based on PICRUSt analysis revealed that 10 functional categories were enriched by avilamycin treatments, and 4 functional categories were decreased.					
29253227	9	71	theme	microbiota	1906:1915	arg1	profiles					1890:1897	functional profiles	1879:1897	functional profiles	1879:1897	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	9	71	theme	microbiota	1906:1915	arg1	diversity					1841:1849	diversity	1841:1849	diversity	1841:1849	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	9	71	theme	microbiota	1906:1915	arg1	composition					1862:1872	taxonomic composition	1852:1872	taxonomic composition	1852:1872	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	3	72	from	ileum	537:541	arg1	chickens					564:571	broiler chickens	556:571	broiler chickens fed with an avilamycin-supplemented diet	556:612	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	9	73	from	cecum	1958:1962	arg1	influence					1794:1802	the influence	1790:1802	the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota	1790:1915	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	9	73	from	cecum	1958:1962	arg1	different					1931:1939	different	1931:1939	different	1931:1939	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	2	74	dep	structure	377:385	arg1	the					373:375	the	373:375	the	373:375	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	3	75	from	cecum	547:551	arg1	chickens					564:571	broiler chickens	556:571	broiler chickens fed with an avilamycin-supplemented diet	556:612	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	0	76	theme	avilamycin	21:30	arg1	Influence					0:8	Influence	0:8	Influence of dietary avilamycin on ileal and cecal microbiota in broiler chickens.	0:81	Influence of dietary avilamycin on ileal and cecal microbiota in broiler chickens.					
29253227	4	77	theme	diversity	680:688	arg1	metrics					690:696	Alpha diversity metrics	674:696	Alpha diversity metrics	674:696	Alpha diversity metrics indicated that the ileal bacterial diversity was higher in avilamycin-fed chickens than in the control group, whereas the opposite was true for the cecum.					
29253227	0	78	theme	ileal	35:39	arg1	microbiota					51:60	ileal and cecal microbiota	35:60	ileal and cecal microbiota in broiler chickens	35:80	Influence of dietary avilamycin on ileal and cecal microbiota in broiler chickens.					
29253227	1	79	theme	alternative	262:272	arg1	strategies					274:283	alternative strategies to replace AGP	262:298	alternative strategies to replace AGP	262:298	The mechanisms by which antibiotic growth promoters (AGP) enhance growth rates, feed efficiencies, and disease resistance in poultry need to be understood for designing safer and alternative strategies to replace AGP.					
29253227	7	80	theme	=	1534:1534	arg1	P					1532:1532	P = 0.005-0.047	1532:1546	P = 0.005-0.047	1532:1546	In the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU, 12 OTU were decreased, and 3 were increased in response to avilamycin treatment (P = 0.005-0.047).					
29253227	7	80	theme	=	1534:1534	arg1	treatment					1521:1529	avilamycin treatment	1510:1529	avilamycin treatment (P = 0.005-0.047)	1510:1547	In the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU, 12 OTU were decreased, and 3 were increased in response to avilamycin treatment (P = 0.005-0.047).					
29253227	2	81	theme	gut	407:409	arg1	microbiome					411:420	the gut microbiome	403:420	the gut microbiome of broiler chickens	403:440	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	3	82	theme	broiler	556:562	arg1	chickens					564:571	broiler chickens	556:571	broiler chickens fed with an avilamycin-supplemented diet	556:612	In this study, we investigated the bacterial communities of the ileum and cecum in broiler chickens fed with an avilamycin-supplemented diet, by high-throughput sequencing of bacterial 16S rRNA genes.					
29253227	0	83	theme	cecal	45:49	arg1	microbiota					51:60	ileal and cecal microbiota	35:60	ileal and cecal microbiota in broiler chickens	35:80	Influence of dietary avilamycin on ileal and cecal microbiota in broiler chickens.					
29253227	6	84	dep	decreased	1344:1352	arg1	P					1355:1355	P = 0.016	1355:1363	P = 0.016	1355:1363	In the ilea, 2 operational taxonomic units (OTU) that matched Lactobacillus reuteri and Clostridium were enriched (P = 0.016 and P = 0.007, respectively) in the avilamycin-fed group, and an OTU belonging to Lactobacillus crispatus was decreased (P = 0.016).					
29253227	7	85	theme	1,286	1426:1430	arg1	OTU					1446:1448	1,286 non-singleton OTU	1426:1448	1,286 non-singleton OTU	1426:1448	In the cecal microbiota showing much higher diversity with 1,286 non-singleton OTU, 12 OTU were decreased, and 3 were increased in response to avilamycin treatment (P = 0.005-0.047).					
29253227	2	86	from	AGP	339:341	arg1	poultry					346:352	poultry	346:352	poultry	346:352	Avilamycin has been widely used as an AGP in poultry, but its impact on the structure and function of the gut microbiome of broiler chickens has not been fully elucidated.					
29253227	9	87	from	ileum	1948:1952	arg1	influence					1794:1802	the influence	1790:1802	the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota	1790:1915	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	9	87	from	ileum	1948:1952	arg1	different					1931:1939	different	1931:1939	different	1931:1939	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	5	88	theme	group	932:936	arg1	microbiota					899:908	the ileal microbiota	889:908	the ileal microbiota of the avilamycin-fed group	889:936	Multivariate analyses revealed that the ileal microbiota of the avilamycin-fed group were clearly distinguished from those of the control group, whereas the cecal bacterial communities were apparently not influenced by feeding diets containing avilamycin.					
29253227	10	89	dep	composition	2033:2043	arg1	the					2029:2031	the	2029:2031	the	2029:2031	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	4	90	theme	bacterial	723:731	arg1	higher					747:752	higher	747:752	higher	747:752	Alpha diversity metrics indicated that the ileal bacterial diversity was higher in avilamycin-fed chickens than in the control group, whereas the opposite was true for the cecum.					
29253227	4	90	theme	bacterial	723:731	arg1	diversity					733:741	the ileal bacterial diversity	713:741	the ileal bacterial diversity	713:741	Alpha diversity metrics indicated that the ileal bacterial diversity was higher in avilamycin-fed chickens than in the control group, whereas the opposite was true for the cecum.					
29253227	8	91	theme	Functional	1550:1559	arg1	profiling					1561:1569	Functional profiling	1550:1569	Functional profiling of bacterial communities based on PICRUSt analysis	1550:1620	Functional profiling of bacterial communities based on PICRUSt analysis revealed that 10 functional categories were enriched by avilamycin treatments, and 4 functional categories were decreased.					
29253227	10	92	from	composition	2033:2043	arg1	tract					2112:2116	the chicken gastrointestinal tract	2083:2116	the chicken gastrointestinal tract	2083:2116	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	10	92	from	composition	2033:2043	arg1	understanding					1991:2003	our understanding	1987:2003	our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance	1987:2196	These results further our understanding of the impact of AGP on the composition and activity of commensal bacteria in the chicken gastrointestinal tract to develop novel feeding strategies for improving animal health and performance.					
29253227	9	93	theme	taxonomic	1852:1860	arg1	composition					1862:1872	taxonomic composition	1852:1872	taxonomic composition	1852:1872	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
29253227	9	94	dep	ileum	1948:1952	arg1	the					1944:1946	the	1944:1946	the	1944:1946	In conclusion, our results demonstrated that the influence of avilamycin supplementation on the diversity, taxonomic composition, and functional profiles of the microbiota was evidently different in the ileum and cecum.					
31744168	0	0	theme	Fasting	66:72	arg1	Period					74:79	a Long Fasting Period	59:79	a Long Fasting Period under a Two-Meals-Per-Day Schedule	59:114	Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule.					
31744168	11	1	theme	consuming	1303:1311	arg1	breakfast					1323:1331	consuming inulin at breakfast	1303:1331	consuming inulin at breakfast	1303:1331	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	9	2	theme	length	1106:1111	arg1	effect					1088:1093	the effect	1084:1093	the effect of fasting length	1084:1111	Furthermore, to confirm the effect of fasting length, mice were housed under a one-meal-per-day schedule.					
31744168	2	3	from	effect	303:308	arg1	microbiota					330:339	microbiota	330:339	microbiota	330:339	Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota.					
31744168	7	4	theme	Inulin	799:804	arg1	feeding					806:812	Inulin feeding	799:812	Inulin feeding in the morning rather than the evening	799:851	Inulin feeding in the morning rather than the evening decreased the cecal pH, increased SCFAs, and changed the microbiome composition.					
31744168	11	5	theme	inulin	1313:1318	arg1	breakfast					1323:1331	consuming inulin at breakfast	1303:1331	consuming inulin at breakfast	1303:1331	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	7	6	theme	cecal	867:871	arg1	pH					873:874	the cecal pH	863:874	the cecal pH	863:874	Inulin feeding in the morning rather than the evening decreased the cecal pH, increased SCFAs, and changed the microbiome composition.					
31744168	10	7	theme	fasting	1187:1193	arg1	equal					1199:1203	equal	1199:1203	equal	1199:1203	When the duration of fasting was equal, the difference between morning and evening nearly disappeared.					
31744168	10	7	theme	fasting	1187:1193	arg1	duration					1175:1182	the duration	1171:1182	the duration of fasting	1171:1193	When the duration of fasting was equal, the difference between morning and evening nearly disappeared.					
31744168	5	8	theme	short-chain	695:705	arg1	SCFAs					720:724	SCFAs	720:724	SCFAs	720:724	Then, 10-14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured.					
31744168	5	8	theme	short-chain	695:705	arg1	acids					713:717	short-chain fatty acids	695:717	short-chain fatty acids (SCFAs)	695:725	Then, 10-14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured.					
31744168	7	9	from	feeding	806:812	arg1	morning					821:827	the morning	817:827	the morning rather than the evening	817:851	Inulin feeding in the morning rather than the evening decreased the cecal pH, increased SCFAs, and changed the microbiome composition.					
31744168	7	9	from	feeding	806:812	arg1	evening					845:851	the evening	841:851	the morning rather than the evening	817:851	Inulin feeding in the morning rather than the evening decreased the cecal pH, increased SCFAs, and changed the microbiome composition.					
31744168	1	10	theme	fecal	166:170	arg1	microbiota					172:181	fecal microbiota	166:181	fecal microbiota	166:181	Water-soluble dietary fiber is known to modulate fecal microbiota.					
31744168	0	11	theme	Two-Meals-Per-Day	89:105	arg1	Schedule					107:114	a Two-Meals-Per-Day Schedule	87:114	a Two-Meals-Per-Day Schedule	87:114	Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule.					
31744168	5	12	theme	fatty	707:711	arg1	SCFAs					720:724	SCFAs	720:724	SCFAs	720:724	Then, 10-14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured.					
31744168	5	12	theme	fatty	707:711	arg1	acids					713:717	short-chain fatty acids	695:717	short-chain fatty acids (SCFAs)	695:725	Then, 10-14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured.					
31744168	9	13	theme	fasting	1098:1104	arg1	length					1106:1111	fasting length	1098:1111	fasting length	1098:1111	Furthermore, to confirm the effect of fasting length, mice were housed under a one-meal-per-day schedule.					
31744168	11	14	from	effect	1398:1403	arg1	microbiota					1412:1421	the microbiota	1408:1421	the microbiota	1408:1421	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	2	15	from	effects	235:241	arg1	metabolism					269:278	metabolism	269:278	metabolism	269:278	Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota.					
31744168	3	16	theme	inulin-containing	402:418	arg1	food					420:423	inulin-containing food	402:423	inulin-containing food	402:423	Therefore, in this study, we examined the timing effects of inulin-containing food on fecal microbiota.					
31744168	0	17	theme	Microbiota	5:14	arg1	Composition					16:26	Mice Microbiota Composition	0:26	Mice Microbiota Composition	0:26	Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule.					
31744168	4	18	theme	fasting	527:533	arg1	period					535:540	a long fasting period	520:540	a long fasting period in the morning	520:555	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31744168	4	19	from	period	577:582	arg1	morning					549:555	the morning	545:555	the morning	545:555	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31744168	4	19	from	period	577:582	arg1	evening					591:597	the evening	587:597	the evening	587:597	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31744168	3	20	theme	timing	384:389	arg1	effects					391:397	the timing effects	380:397	the timing effects of inulin-containing food on fecal microbiota	380:443	Therefore, in this study, we examined the timing effects of inulin-containing food on fecal microbiota.					
31744168	0	21	theme	Mice	0:3	arg1	Composition					16:26	Mice Microbiota Composition	0:26	Mice Microbiota Composition	0:26	Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule.					
31744168	4	22	theme	long	522:525	arg1	period					535:540	a long fasting period	520:540	a long fasting period in the morning	520:555	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31744168	9	23	theme	one-meal-per-day	1139:1154	arg1	schedule					1156:1163	a one-meal-per-day schedule	1137:1163	a one-meal-per-day schedule	1137:1163	Furthermore, to confirm the effect of fasting length, mice were housed under a one-meal-per-day schedule.					
31744168	11	24	contain	has	1384:1386	arg2	effect					1398:1403	a greater effect	1388:1403	a greater effect on the microbiota	1388:1421	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	11	24	contain	has	1384:1386	arg1	breakfast					1323:1331	consuming inulin at breakfast	1303:1331	consuming inulin at breakfast	1303:1331	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	5	25	theme	cecal	638:642	arg1	content					644:650	cecal content	638:650	cecal content	638:650	Then, 10-14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured.					
31744168	3	26	theme	fecal	428:432	arg1	microbiota					434:443	fecal microbiota	428:443	fecal microbiota	428:443	Therefore, in this study, we examined the timing effects of inulin-containing food on fecal microbiota.					
31744168	11	27	theme	at	1320:1321	arg1	breakfast					1323:1331	consuming inulin at breakfast	1303:1331	consuming inulin at breakfast	1303:1331	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	8	28	theme	inactive	1043:1050	arg1	period					1052:1057	the inactive period	1039:1057	the inactive period	1039:1057	These data suggest that inulin is more easily digested by fecal microbiota during the active period than the inactive period.					
31744168	0	29	theme	Inulin	39:44	arg1	Feeding					46:52	Inulin Feeding	39:52	Inulin Feeding	39:52	Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule.					
31744168	2	30	theme	intake	313:318	arg1	timing					320:325	intake timing	313:325	intake timing	313:325	Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota.					
31744168	6	31	theme	rDNA	782:785	arg1	sequencing					787:796	16S rDNA sequencing	778:796	16S rDNA sequencing	778:796	The microbiome was determined using 16S rDNA sequencing.					
31744168	3	32	theme	food	420:423	arg1	effects					391:397	the timing effects	380:397	the timing effects of inulin-containing food on fecal microbiota	380:443	Therefore, in this study, we examined the timing effects of inulin-containing food on fecal microbiota.					
31744168	5	33	theme	cecal	682:686	arg1	pH					688:689	cecal pH	682:689	cecal pH	682:689	Then, 10-14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured.					
31744168	8	34	theme	fecal	992:996	arg1	microbiota					998:1007	fecal microbiota	992:1007	fecal microbiota	992:1007	These data suggest that inulin is more easily digested by fecal microbiota during the active period than the inactive period.					
31744168	6	35	theme	16S	778:780	arg1	sequencing					787:796	16S rDNA sequencing	778:796	16S rDNA sequencing	778:796	The microbiome was determined using 16S rDNA sequencing.					
31744168	4	36	theme	two-meals-per-day	487:503	arg1	schedule					505:512	a two-meals-per-day schedule	485:512	a two-meals-per-day schedule	485:512	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31744168	2	37	theme	timing	320:325	arg1	effect					303:308	the effect	299:308	the effect of intake timing on microbiota	299:339	Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota.					
31744168	5	38	theme	inulin	623:628	arg1	intake					630:635	inulin intake	623:635	inulin intake	623:635	Then, 10-14 days after inulin intake, cecal content and feces were collected, and cecal pH and short-chain fatty acids (SCFAs) were measured.					
31744168	2	39	theme	few	205:207	arg1	reports					209:215	a few reports	203:215	a few reports investigating the effects of fiber intake timing on metabolism	203:278	Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota.					
31744168	1	40	theme	Water-soluble	117:129	arg1	fiber					139:143	Water-soluble dietary fiber	117:143	Water-soluble dietary fiber	117:143	Water-soluble dietary fiber is known to modulate fecal microbiota.					
31744168	2	41	theme	timing	259:264	arg1	effects					235:241	the effects	231:241	the effects of fiber intake timing on metabolism	231:278	Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota.					
31744168	7	42	theme	microbiome	910:919	arg1	composition					921:931	the microbiome composition	906:931	the microbiome composition	906:931	Inulin feeding in the morning rather than the evening decreased the cecal pH, increased SCFAs, and changed the microbiome composition.					
31744168	1	43	theme	dietary	131:137	arg1	fiber					139:143	Water-soluble dietary fiber	117:143	Water-soluble dietary fiber	117:143	Water-soluble dietary fiber is known to modulate fecal microbiota.					
31744168	4	44	theme	short	563:567	arg1	period					577:582	a short fasting period	561:582	a short fasting period in the evening	561:597	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31744168	8	45	theme	active	1020:1025	arg1	period					1027:1032	the active period	1016:1032	the active period than the inactive period	1016:1057	These data suggest that inulin is more easily digested by fecal microbiota during the active period than the inactive period.					
31744168	2	46	theme	intake	252:257	arg1	timing					259:264	fiber intake timing	246:264	fiber intake timing	246:264	Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota.					
31744168	11	47	theme	longer	1361:1366	arg1	period					1376:1381	a longer fasting period	1359:1381	a longer fasting period	1359:1381	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	4	48	with	conditions	469:478	arg1	schedule					505:512	a two-meals-per-day schedule	485:512	a two-meals-per-day schedule	485:512	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31744168	0	49	theme	Long	61:64	arg1	Period					74:79	a Long Fasting Period	59:79	a Long Fasting Period under a Two-Meals-Per-Day Schedule	59:114	Mice Microbiota Composition Changes by Inulin Feeding with a Long Fasting Period under a Two-Meals-Per-Day Schedule.					
31744168	11	50	from	breakfast	1323:1331	arg1	at					1320:1321	consuming inulin at breakfast	1303:1331	consuming inulin at breakfast	1303:1331	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	11	50	from	breakfast	1323:1331	arg1	inulin					1313:1318	consuming inulin at breakfast	1303:1331	consuming inulin at breakfast	1303:1331	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	2	51	theme	fiber	246:250	arg1	timing					259:264	fiber intake timing	246:264	fiber intake timing	246:264	Although there are a few reports investigating the effects of fiber intake timing on metabolism, there are none on the effect of intake timing on microbiota.					
31744168	3	52	from	effects	391:397	arg1	microbiota					434:443	fecal microbiota	428:443	fecal microbiota	428:443	Therefore, in this study, we examined the timing effects of inulin-containing food on fecal microbiota.					
31744168	11	53	theme	fasting	1368:1374	arg1	period					1376:1381	a longer fasting period	1359:1381	a longer fasting period	1359:1381	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	4	54	from	period	535:540	arg1	morning					549:555	the morning	545:555	the morning	545:555	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31744168	4	54	from	period	535:540	arg1	evening					591:597	the evening	587:597	the evening	587:597	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31744168	11	55	theme	greater	1390:1396	arg1	effect					1398:1403	a greater effect	1388:1403	a greater effect on the microbiota	1388:1421	Thus, our study demonstrates that consuming inulin at breakfast, which is generally after a longer fasting period, has a greater effect on the microbiota.					
31744168	4	56	theme	fasting	569:575	arg1	period					577:582	a short fasting period	561:582	a short fasting period in the evening	561:597	Mice were housed under conditions with a two-meals-per-day schedule, with a long fasting period in the morning and a short fasting period in the evening.					
31381982	4	0	theme	spore	995:999	arg1	suspensions					1001:1011	spore suspensions	995:1011	spore suspensions of increasing endospore concentration	995:1049	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	5	1	theme	distribution	1149:1160	arg1	evenness					1130:1137	the evenness	1126:1137	the evenness of colony distribution across replicate plates for four industrial Bacillus isolates	1126:1222	Additionally, we examined the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates.					
31381982	4	2	theme	Bacillus	881:888	arg1	isolates					890:897	swarming industrial Bacillus isolates	861:897	swarming industrial Bacillus isolates	861:897	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	7	3	theme	standard	1515:1522	arg1	protocols					1524:1532	standard protocols	1515:1532	standard protocols for the enumeration of Bacillus-based products	1515:1579	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	1	4	theme	widespread	162:171	arg1	methods					173:179	the most widespread methods	153:179	the most widespread methods for enumerating industrial Bacillus assemblages	153:227	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	0	5	theme	plate-counting	86:99	arg1	assays					101:106	customized plate-counting assays	75:106	customized plate-counting assays	75:106	Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays.					
31381982	3	6	theme	mixed-species	697:709	arg1	assemblage					711:720	a mixed-species assemblage	695:720	a mixed-species assemblage	695:720	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	4	7	theme	industrial	870:879	arg1	isolates					890:897	swarming industrial Bacillus isolates	861:897	swarming industrial Bacillus isolates	861:897	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	4	8	theme	isolates	890:897	arg1	assemblage					847:856	an assemblage	844:856	an assemblage of swarming industrial Bacillus isolates	844:897	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	6	9	theme	axenic	1340:1345	arg1	suspensions					1357:1367	axenic endospore suspensions	1340:1367	axenic endospore suspensions	1340:1367	We compared the observed distribution of colonies across replicate plates to the expected Poisson distribution for axenic endospore suspensions, for a 3-species assemblage and a 4-species assemblage, respectively.					
31381982	4	10	theme	swarming	953:960	arg1	inhibitor					962:970	a swarming inhibitor	951:970	a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration	951:1049	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	0	11	from	Enumeration	0:10	arg1	products					61:68	commercial products	50:68	commercial products	50:68	Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays.					
31381982	7	12	from	colonies	1612:1619	arg1	media					1630:1634	solid media	1624:1634	solid media	1624:1634	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	4	13	theme	present	779:785	arg1	study					787:791	the present study	775:791	the present study	775:791	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	7	14	theme	customized	1460:1469	arg1	assays					1479:1484	customized plating assays	1460:1484	customized plating assays	1460:1484	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	7	15	theme	solid	1624:1628	arg1	media					1630:1634	solid media	1624:1634	solid media	1624:1634	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	7	16	theme	plating	1471:1477	arg1	assays					1479:1484	customized plating assays	1460:1484	customized plating assays	1460:1484	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	6	17	dep	plates	1292:1297	arg1	replicate					1282:1290	replicate	1282:1290	replicate	1282:1290	We compared the observed distribution of colonies across replicate plates to the expected Poisson distribution for axenic endospore suspensions, for a 3-species assemblage and a 4-species assemblage, respectively.					
31381982	3	18	theme	quantification	571:584	arg1	limits					561:566	established limits	549:566	established limits of quantification	549:584	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	6	19	theme	Poisson	1315:1321	arg1	distribution					1323:1334	the expected Poisson distribution	1302:1334	the expected Poisson distribution for axenic endospore suspensions	1302:1367	We compared the observed distribution of colonies across replicate plates to the expected Poisson distribution for axenic endospore suspensions, for a 3-species assemblage and a 4-species assemblage, respectively.					
31381982	2	20	theme	CFU	374:376	arg1	count					378:382	the CFU count	370:382	the CFU count of Bacillus-based products	370:409	However, the standard plating assays used to verify the CFU count of Bacillus-based products are not tailored to Bacillus species and thus may not produce the most accurate possible estimations.					
31381982	6	21	theme	3-species	1376:1384	arg1	assemblage					1386:1395	a 3-species assemblage	1374:1395	a 3-species assemblage	1374:1395	We compared the observed distribution of colonies across replicate plates to the expected Poisson distribution for axenic endospore suspensions, for a 3-species assemblage and a 4-species assemblage, respectively.					
31381982	6	22	theme	4-species	1403:1411	arg1	assemblage					1413:1422	a 4-species assemblage	1401:1422	a 4-species assemblage	1401:1422	We compared the observed distribution of colonies across replicate plates to the expected Poisson distribution for axenic endospore suspensions, for a 3-species assemblage and a 4-species assemblage, respectively.					
31381982	6	23	theme	expected	1306:1313	arg1	distribution					1323:1334	the expected Poisson distribution	1302:1334	the expected Poisson distribution for axenic endospore suspensions	1302:1367	We compared the observed distribution of colonies across replicate plates to the expected Poisson distribution for axenic endospore suspensions, for a 3-species assemblage and a 4-species assemblage, respectively.					
31381982	3	24	theme	established	549:559	arg1	limits					561:566	established limits	549:566	established limits of quantification	549:584	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	2	25	theme	products	402:409	arg1	count					378:382	the CFU count	370:382	the CFU count of Bacillus-based products	370:409	However, the standard plating assays used to verify the CFU count of Bacillus-based products are not tailored to Bacillus species and thus may not produce the most accurate possible estimations.					
31381982	4	26	theme	increasing	1016:1025	arg1	concentration					1037:1049	increasing endospore concentration	1016:1049	increasing endospore concentration	1016:1049	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	5	27	dep	plates	1179:1184	arg1	replicate					1169:1177	replicate	1169:1177	replicate	1169:1177	Additionally, we examined the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates.					
31381982	3	28	from	swarm	636:640	arg1	media					651:655	solid media	645:655	solid media	645:655	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	3	28	from	swarm	636:640	arg1	assemblage					711:720	a mixed-species assemblage	695:720	a mixed-species assemblage	695:720	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	1	29	theme	enumerating	185:195	arg1	assemblages					217:227	enumerating industrial Bacillus assemblages	185:227	enumerating industrial Bacillus assemblages	185:227	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	2	30	theme	possible	491:498	arg1	estimations					500:510	the most accurate possible estimations	473:510	the most accurate possible estimations	473:510	However, the standard plating assays used to verify the CFU count of Bacillus-based products are not tailored to Bacillus species and thus may not produce the most accurate possible estimations.					
31381982	4	31	theme	swarming	861:868	arg1	isolates					890:897	swarming industrial Bacillus isolates	861:897	swarming industrial Bacillus isolates	861:897	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	2	32	theme	plating	340:346	arg1	assays					348:353	the standard plating assays	327:353	the standard plating assays used to verify the CFU count of Bacillus-based products	327:409	However, the standard plating assays used to verify the CFU count of Bacillus-based products are not tailored to Bacillus species and thus may not produce the most accurate possible estimations.					
31381982	1	33	theme	industrial	197:206	arg1	assemblages					217:227	enumerating industrial Bacillus assemblages	185:227	enumerating industrial Bacillus assemblages	185:227	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	4	34	theme	concentration	1037:1049	arg1	suspensions					1001:1011	spore suspensions	995:1011	spore suspensions of increasing endospore concentration	995:1049	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	5	35	theme	Bacillus	1206:1213	arg1	isolates					1215:1222	four industrial Bacillus isolates	1190:1222	four industrial Bacillus isolates	1190:1222	Additionally, we examined the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates.					
31381982	1	36	from	areas	311:315	arg1	prohibitive					276:286	prohibitive	276:286	prohibitive	276:286	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	7	37	theme	Bacillus-based	1557:1570	arg1	products					1572:1579	Bacillus-based products	1557:1579	Bacillus-based products	1557:1579	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	2	38	theme	standard	331:338	arg1	assays					348:353	the standard plating assays	327:353	the standard plating assays used to verify the CFU count of Bacillus-based products	327:409	However, the standard plating assays used to verify the CFU count of Bacillus-based products are not tailored to Bacillus species and thus may not produce the most accurate possible estimations.					
31381982	1	39	theme	Bacillus	208:215	arg1	assemblages					217:227	enumerating industrial Bacillus assemblages	185:227	enumerating industrial Bacillus assemblages	185:227	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	0	40	theme	Bacillus	26:33	arg1	assemblages					35:45	industrial Bacillus assemblages	15:45	industrial Bacillus assemblages	15:45	Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays.					
31381982	1	41	theme	aerobic	113:119	arg1	assay					133:137	The aerobic plate count assay	109:137	The aerobic plate count assay	109:137	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	0	42	theme	industrial	15:24	arg1	assemblages					35:45	industrial Bacillus assemblages	15:45	industrial Bacillus assemblages	15:45	Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays.					
31381982	3	43	theme	agar	759:762	arg1	plates					764:769	agar plates	759:769	agar plates	759:769	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	1	44	theme	plate	121:125	arg1	assay					133:137	The aerobic plate count assay	109:137	The aerobic plate count assay	109:137	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	2	45	theme	Bacillus	431:438	arg1	species					440:446	Bacillus species	431:446	Bacillus species	431:446	However, the standard plating assays used to verify the CFU count of Bacillus-based products are not tailored to Bacillus species and thus may not produce the most accurate possible estimations.					
31381982	2	46	theme	accurate	482:489	arg1	estimations					500:510	the most accurate possible estimations	473:510	the most accurate possible estimations	473:510	However, the standard plating assays used to verify the CFU count of Bacillus-based products are not tailored to Bacillus species and thus may not produce the most accurate possible estimations.					
31381982	1	47	from	prohibitive	276:286	arg1	areas					311:315	some areas	306:315	some areas	306:315	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	5	48	theme	assemblage	1092:1101	arg1	composition					1111:1121	assemblage species composition	1092:1121	assemblage species composition	1092:1121	Additionally, we examined the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates.					
31381982	2	49	theme	Bacillus-based	387:400	arg1	products					402:409	Bacillus-based products	387:409	Bacillus-based products	387:409	However, the standard plating assays used to verify the CFU count of Bacillus-based products are not tailored to Bacillus species and thus may not produce the most accurate possible estimations.					
31381982	0	50	theme	assemblages	35:45	arg1	Enumeration					0:10	Enumeration	0:10	Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays.	0:107	Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays.					
31381982	7	51	theme	mixed-species	1692:1704	arg1	assemblages					1715:1725	mixed-species Bacillus assemblages	1692:1725	mixed-species Bacillus assemblages	1692:1725	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	5	52	theme	species	1103:1109	arg1	composition					1111:1121	assemblage species composition	1092:1121	assemblage species composition	1092:1121	Additionally, we examined the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates.					
31381982	1	53	theme	growth-independent	233:250	arg1	methods					252:258	growth-independent methods	233:258	growth-independent methods	233:258	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	4	54	theme	endospore	1027:1035	arg1	concentration					1037:1049	increasing endospore concentration	1016:1049	increasing endospore concentration	1016:1049	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	3	55	theme	plating	522:528	arg1	assays					530:535	Standard plating assays	513:535	Standard plating assays	513:535	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	4	56	theme	plate	912:916	arg1	count					918:922	plate count	912:922	plate count	912:922	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	5	57	theme	composition	1111:1121	arg1	impact					1082:1087	the impact	1078:1087	the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates	1078:1222	Additionally, we examined the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates.					
31381982	6	58	theme	colonies	1266:1273	arg1	distribution					1250:1261	the observed distribution	1237:1261	the observed distribution of colonies across replicate plates	1237:1297	We compared the observed distribution of colonies across replicate plates to the expected Poisson distribution for axenic endospore suspensions, for a 3-species assemblage and a 4-species assemblage, respectively.					
31381982	1	59	theme	count	127:131	arg1	assay					133:137	The aerobic plate count assay	109:137	The aerobic plate count assay	109:137	The aerobic plate count assay remains among the most widespread methods for enumerating industrial Bacillus assemblages, as growth-independent methods are either cost prohibitive or unavailable in some areas.					
31381982	0	60	theme	commercial	50:59	arg1	products					61:68	commercial products	50:68	commercial products	50:68	Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays.					
31381982	4	61	theme	quantification	825:838	arg1	limit					816:820	the upper limit	806:820	the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates	806:897	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	3	62	theme	Standard	513:520	arg1	assays					530:535	Standard plating assays	513:535	Standard plating assays	513:535	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	5	63	theme	industrial	1195:1204	arg1	isolates					1215:1222	four industrial Bacillus isolates	1190:1222	four industrial Bacillus isolates	1190:1222	Additionally, we examined the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates.					
31381982	5	64	from	impact	1082:1087	arg1	evenness					1130:1137	the evenness	1126:1137	the evenness of colony distribution across replicate plates for four industrial Bacillus isolates	1126:1222	Additionally, we examined the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates.					
31381982	0	65	with	Enumeration	0:10	arg1	assays					101:106	customized plate-counting assays	75:106	customized plate-counting assays	75:106	Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays.					
31381982	6	66	theme	endospore	1347:1355	arg1	suspensions					1357:1367	axenic endospore suspensions	1340:1367	axenic endospore suspensions	1340:1367	We compared the observed distribution of colonies across replicate plates to the expected Poisson distribution for axenic endospore suspensions, for a 3-species assemblage and a 4-species assemblage, respectively.					
31381982	7	67	theme	products	1572:1579	arg1	enumeration					1542:1552	the enumeration	1538:1552	the enumeration of Bacillus-based products	1538:1579	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	6	68	theme	observed	1241:1248	arg1	distribution					1250:1261	the observed distribution	1237:1261	the observed distribution of colonies across replicate plates	1237:1297	We compared the observed distribution of colonies across replicate plates to the expected Poisson distribution for axenic endospore suspensions, for a 3-species assemblage and a 4-species assemblage, respectively.					
31381982	7	69	theme	Bacillus	1706:1713	arg1	assemblages					1715:1725	mixed-species Bacillus assemblages	1692:1725	mixed-species Bacillus assemblages	1692:1725	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	7	70	theme	plating	1675:1681	arg1	data					1683:1686	plating data	1675:1686	plating data for mixed-species Bacillus assemblages	1675:1725	Results suggest that customized plating assays may be more appropriate than standard protocols for the enumeration of Bacillus-based products, and that interactions between colonies on solid media should be considered when interpreting plating data for mixed-species Bacillus assemblages.					
31381982	0	71	theme	customized	75:84	arg1	assays					101:106	customized plate-counting assays	75:106	customized plate-counting assays	75:106	Enumeration of industrial Bacillus assemblages in commercial products with customized plate-counting assays.					
31381982	3	72	theme	species	684:690	arg1	colonies					667:674	colonies	667:674	colonies of each species	667:690	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	4	73	theme	upper	810:814	arg1	limit					816:820	the upper limit	806:820	the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates	806:897	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	4	74	with	inhibitor	962:970	arg1	counts					984:989	direct counts	977:989	direct counts for spore suspensions of increasing endospore concentration	977:1049	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	4	75	theme	direct	977:982	arg1	counts					984:989	direct counts	977:989	direct counts for spore suspensions of increasing endospore concentration	977:1049	In the present study, we examined the upper limit of quantification for an assemblage of swarming industrial Bacillus isolates by comparing plate count on medium with and without a swarming inhibitor with direct counts for spore suspensions of increasing endospore concentration.					
31381982	5	76	theme	colony	1142:1147	arg1	distribution					1149:1160	colony distribution	1142:1160	colony distribution across replicate plates for four industrial Bacillus isolates	1142:1222	Additionally, we examined the impact of assemblage species composition on the evenness of colony distribution across replicate plates for four industrial Bacillus isolates.					
31381982	3	77	theme	solid	645:649	arg1	media					651:655	solid media	645:655	solid media	645:655	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	3	78	theme	Bacillus	604:611	arg1	species					613:619	Bacillus species	604:619	Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates	604:769	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31381982	3	79	dep	one	744:746	arg1	another					748:754	another	748:754	another	748:754	Standard plating assays assume that established limits of quantification are applicable to Bacillus species whose colonies swarm on solid media, and that colonies of each species in a mixed-species assemblage form independently of one another on agar plates.					
31066660	8	0	theme	DNA	1219:1221	arg1	imprecise					1283:1291	imprecise	1283:1291	imprecise	1283:1291	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	8	0	theme	DNA	1219:1221	arg1	G+C contents					1223:1234	the genomic DNA G+C contents	1207:1234	the genomic DNA G+C contents mentioned in some species descriptions	1207:1273	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	9	1	theme	species	1420:1426	arg1	descriptions					1428:1439	species descriptions	1420:1439	species descriptions	1420:1439	Thus, the corresponding emendations of species descriptions are also proposed.					
31066660	8	2	from	imprecise	1283:1291	arg1	addition					1135:1142	addition	1135:1142	addition	1135:1142	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	1	3	theme	taxonomic	191:199	arg1	study					201:205	a polyphasic taxonomic study	178:205	a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics	178:285	The family Thermoactinomycetaceaecomprises 43 validly published species, which were identified by a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics.					
31066660	5	4	theme	guangxiensis	999:1010	arg1	sediminis					839:847	Laceyella sediminis	829:847	Laceyella sediminis	829:847	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	4	theme	guangxiensis	999:1010	arg1	comb					1023:1026	comb	1023:1026	comb	1023:1026	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	4	theme	guangxiensis	999:1010	arg1	nov.					1017:1020	Paenactinomyces guangxiensis gen. nov.	983:1020	Paenactinomyces guangxiensis gen. nov.	983:1020	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	8	5	theme	corrected	1302:1310	arg1	values					1324:1329	the corrected G+C content values	1298:1329	the corrected G+C content values	1298:1329	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	4	6	from	those	647:651	arg1	different					632:640	different	632:640	different	632:640	The topologies of phylogenomic trees are different from those of the 16S rRNA gene sequences.					
31066660	4	6	from	those	647:651	arg1	topologies					595:604	The topologies	591:604	The topologies of phylogenomic trees	591:626	The topologies of phylogenomic trees are different from those of the 16S rRNA gene sequences.					
31066660	3	7	used	used	487:490	arg2	we					484:485	we	484:485	we	484:485	For disentangling the phylogenetic relationships among members of this family, we used a large collection of genome data and the approach of phylogenomics, to re-examine their taxonomy.					
31066660	5	8	theme	phenotypic	771:780	arg1	identity					730:737	the average nucleotide identity	707:737	the average nucleotide identity	707:737	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	8	theme	phenotypic	771:780	arg1	characteristics					798:812	phenotypic and biochemical characteristics	771:812	phenotypic and biochemical characteristics	771:812	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	4	9	theme	gene	669:672	arg1	sequences					674:682	the 16S rRNA gene sequences	656:682	the 16S rRNA gene sequences	656:682	The topologies of phylogenomic trees are different from those of the 16S rRNA gene sequences.					
31066660	2	10	theme	rRNA	339:342	arg1	sequences					349:357	16S rRNA gene sequences	335:357	16S rRNA gene sequences	335:357	However, phylogenetic analysis merely based on 16S rRNA gene sequences cannot infer a robust and reliable phylogeny.					
31066660	3	11	theme	phylogenomics	546:558	arg1	approach					534:541	the approach	530:541	the approach of phylogenomics	530:558	For disentangling the phylogenetic relationships among members of this family, we used a large collection of genome data and the approach of phylogenomics, to re-examine their taxonomy.					
31066660	3	11	theme	phylogenomics	546:558	arg1	collection					500:509	a large collection	492:509	a large collection of genome data	492:524	For disentangling the phylogenetic relationships among members of this family, we used a large collection of genome data and the approach of phylogenomics, to re-examine their taxonomy.					
31066660	5	12	theme	heterotypic	883:893	arg1	synonym					895:901	a later heterotypic synonym	875:901	a later heterotypic synonym of Laceyella tengchongensis	875:929	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	12	theme	heterotypic	883:893	arg1	sediminis					839:847	Laceyella sediminis	829:847	Laceyella sediminis	829:847	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	3	13	theme	data	521:524	arg1	approach					534:541	the approach	530:541	the approach of phylogenomics	530:558	For disentangling the phylogenetic relationships among members of this family, we used a large collection of genome data and the approach of phylogenomics, to re-examine their taxonomy.					
31066660	3	13	theme	data	521:524	arg1	collection					500:509	a large collection	492:509	a large collection of genome data	492:524	For disentangling the phylogenetic relationships among members of this family, we used a large collection of genome data and the approach of phylogenomics, to re-examine their taxonomy.					
31066660	2	14	theme	16S	335:337	arg1	sequences					349:357	16S rRNA gene sequences	335:357	16S rRNA gene sequences	335:357	However, phylogenetic analysis merely based on 16S rRNA gene sequences cannot infer a robust and reliable phylogeny.					
31066660	5	15	theme	Laceyella	906:914	arg1	tengchongensis					916:929	Laceyella tengchongensis	906:929	Laceyella tengchongensis	906:929	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	16	theme	biochemical	786:796	arg1	identity					730:737	the average nucleotide identity	707:737	the average nucleotide identity	707:737	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	16	theme	biochemical	786:796	arg1	characteristics					798:812	phenotypic and biochemical characteristics	771:812	phenotypic and biochemical characteristics	771:812	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	8	17	contain	have	1331:1334	arg1	values					1324:1329	the corrected G+C content values	1298:1329	the corrected G+C content values	1298:1329	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	8	17	contain	have	1331:1334	arg2	fit					1359:1361	a significantly better fit	1336:1361	a significantly better fit to the phylogeny	1336:1378	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	8	18	theme	species	1254:1260	arg1	descriptions					1262:1273	some species descriptions	1249:1273	some species descriptions	1249:1273	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	3	19	theme	large	494:498	arg1	collection					500:509	a large collection	492:509	a large collection of genome data	492:524	For disentangling the phylogenetic relationships among members of this family, we used a large collection of genome data and the approach of phylogenomics, to re-examine their taxonomy.					
31066660	0	20	theme	taxonomic	13:21	arg1	classification					23:36	Genome-based taxonomic classification	0:36	Genome-based taxonomic classification within the family	0:54	Genome-based taxonomic classification within the family Thermoactinomycetaceae.					
31066660	8	21	theme	genomic	1211:1217	arg1	imprecise					1283:1291	imprecise	1283:1291	imprecise	1283:1291	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	8	21	theme	genomic	1211:1217	arg1	G+C contents					1223:1234	the genomic DNA G+C contents	1207:1234	the genomic DNA G+C contents mentioned in some species descriptions	1207:1273	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	10	22	used	used	1498:1501	arg2	phylogenomics					1475:1487	phylogenomics	1475:1487	phylogenomics	1475:1487	In this paper, phylogenomics has been used to resolve the classification of the family Thermoactinomycetaceae.					
31066660	1	23	theme	molecular	216:224	arg1	characteristics					271:285	physiological and biochemical characteristics	241:285	physiological and biochemical characteristics	241:285	The family Thermoactinomycetaceaecomprises 43 validly published species, which were identified by a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics.					
31066660	1	23	theme	molecular	216:224	arg1	phylogenetics					226:238	molecular phylogenetics	216:238	molecular phylogenetics	216:238	The family Thermoactinomycetaceaecomprises 43 validly published species, which were identified by a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics.					
31066660	0	24	theme	Genome-based	0:11	arg1	classification					23:36	Genome-based taxonomic classification	0:36	Genome-based taxonomic classification within the family	0:54	Genome-based taxonomic classification within the family Thermoactinomycetaceae.					
31066660	6	25	theme	Novibacillaceae	1049:1063	arg1	fam					1065:1067	Novibacillaceae fam	1049:1067	Novibacillaceae fam	1049:1067	nov.; and establish Novibacillaceae fam.					
31066660	8	26	theme	G+C content	1312:1322	arg1	values					1324:1329	the corrected G+C content values	1298:1329	the corrected G+C content values	1298:1329	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	1	27	theme	published	134:142	arg1	species					144:150	43 validly published species	123:150	43 validly published species	123:150	The family Thermoactinomycetaceaecomprises 43 validly published species, which were identified by a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics.					
31066660	3	28	theme	phylogenetic	427:438	arg1	relationships					440:452	the phylogenetic relationships	423:452	the phylogenetic relationships among members of this family	423:481	For disentangling the phylogenetic relationships among members of this family, we used a large collection of genome data and the approach of phylogenomics, to re-examine their taxonomy.					
31066660	3	29	theme	genome	514:519	arg1	data					521:524	genome data	514:524	genome data	514:524	For disentangling the phylogenetic relationships among members of this family, we used a large collection of genome data and the approach of phylogenomics, to re-examine their taxonomy.					
31066660	4	30	theme	trees	622:626	arg1	different					632:640	different	632:640	different	632:640	The topologies of phylogenomic trees are different from those of the 16S rRNA gene sequences.					
31066660	4	30	theme	trees	622:626	arg1	topologies					595:604	The topologies	591:604	The topologies of phylogenomic trees	591:626	The topologies of phylogenomic trees are different from those of the 16S rRNA gene sequences.					
31066660	5	31	theme	digital	740:746	arg1	identity					730:737	the average nucleotide identity	707:737	the average nucleotide identity	707:737	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	31	theme	digital	740:746	arg1	hybridization					756:768	digital DNA-DNA hybridization	740:768	digital DNA-DNA hybridization	740:768	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	1	32	theme	physiological	241:253	arg1	characteristics					271:285	physiological and biochemical characteristics	241:285	physiological and biochemical characteristics	241:285	The family Thermoactinomycetaceaecomprises 43 validly published species, which were identified by a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics.					
31066660	1	32	theme	physiological	241:253	arg1	phylogenetics					226:238	molecular phylogenetics	216:238	molecular phylogenetics	216:238	The family Thermoactinomycetaceaecomprises 43 validly published species, which were identified by a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics.					
31066660	3	33	theme	family	476:481	arg1	members					460:466	members	460:466	members of this family	460:481	For disentangling the phylogenetic relationships among members of this family, we used a large collection of genome data and the approach of phylogenomics, to re-examine their taxonomy.					
31066660	4	34	theme	phylogenomic	609:620	arg1	trees					622:626	phylogenomic trees	609:626	phylogenomic trees	609:626	The topologies of phylogenomic trees are different from those of the 16S rRNA gene sequences.					
31066660	5	35	theme	DNA-DNA	748:754	arg1	identity					730:737	the average nucleotide identity	707:737	the average nucleotide identity	707:737	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	35	theme	DNA-DNA	748:754	arg1	hybridization					756:768	digital DNA-DNA hybridization	740:768	digital DNA-DNA hybridization	740:768	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	36	theme	Laceyella	829:837	arg1	sediminis					839:847	Laceyella sediminis	829:847	Laceyella sediminis	829:847	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	36	theme	Laceyella	829:837	arg1	synonym					895:901	a later heterotypic synonym	875:901	a later heterotypic synonym of Laceyella tengchongensis	875:929	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	36	theme	Laceyella	829:837	arg1	nov.					1017:1020	Paenactinomyces guangxiensis gen. nov.	983:1020	Paenactinomyces guangxiensis gen. nov.	983:1020	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	0	37	dep	Thermoactinomycetaceae	56:77	arg1	classification					23:36	Genome-based taxonomic classification	0:36	Genome-based taxonomic classification within the family	0:54	Genome-based taxonomic classification within the family Thermoactinomycetaceae.					
31066660	7	38	theme	genus	1094:1098	arg1	Novibacillus					1100:1111	the genus Novibacillus	1090:1111	the genus Novibacillus as the type genus	1090:1129	nov. to accommodate the genus Novibacillus as the type genus.					
31066660	1	39	theme	biochemical	259:269	arg1	characteristics					271:285	physiological and biochemical characteristics	241:285	physiological and biochemical characteristics	241:285	The family Thermoactinomycetaceaecomprises 43 validly published species, which were identified by a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics.					
31066660	1	39	theme	biochemical	259:269	arg1	phylogenetics					226:238	molecular phylogenetics	216:238	molecular phylogenetics	216:238	The family Thermoactinomycetaceaecomprises 43 validly published species, which were identified by a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics.					
31066660	8	40	from	addition	1135:1142	arg1	G+C contents					1223:1234	the genomic DNA G+C contents	1207:1234	the genomic DNA G+C contents mentioned in some species descriptions	1207:1273	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	8	40	from	addition	1135:1142	arg1	imprecise					1283:1291	imprecise	1283:1291	imprecise	1283:1291	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	7	41	theme	type	1120:1123	arg1	genus					1125:1129	the type genus	1116:1129	the type genus	1116:1129	nov. to accommodate the genus Novibacillus as the type genus.					
31066660	5	42	theme	Paenactinomyces	983:997	arg1	sediminis					839:847	Laceyella sediminis	829:847	Laceyella sediminis	829:847	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	42	theme	Paenactinomyces	983:997	arg1	comb					1023:1026	comb	1023:1026	comb	1023:1026	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	42	theme	Paenactinomyces	983:997	arg1	nov.					1017:1020	Paenactinomyces guangxiensis gen. nov.	983:1020	Paenactinomyces guangxiensis gen. nov.	983:1020	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	43	theme	average	711:717	arg1	identity					730:737	the average nucleotide identity	707:737	the average nucleotide identity	707:737	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	43	theme	average	711:717	arg1	characteristics					798:812	phenotypic and biochemical characteristics	771:812	phenotypic and biochemical characteristics	771:812	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	43	theme	average	711:717	arg1	hybridization					756:768	digital DNA-DNA hybridization	740:768	digital DNA-DNA hybridization	740:768	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	10	44	theme	family	1540:1545	arg1	Thermoactinomycetaceae					1547:1568	the family Thermoactinomycetaceae	1536:1568	the family Thermoactinomycetaceae	1536:1568	In this paper, phylogenomics has been used to resolve the classification of the family Thermoactinomycetaceae.					
31066660	2	45	theme	reliable	385:392	arg1	phylogeny					394:402	a robust and reliable phylogeny	372:402	a robust and reliable phylogeny	372:402	However, phylogenetic analysis merely based on 16S rRNA gene sequences cannot infer a robust and reliable phylogeny.					
31066660	10	46	theme	Thermoactinomycetaceae	1547:1568	arg1	classification					1518:1531	the classification	1514:1531	the classification of the family Thermoactinomycetaceae	1514:1568	In this paper, phylogenomics has been used to resolve the classification of the family Thermoactinomycetaceae.					
31066660	5	47	theme	nucleotide	719:728	arg1	identity					730:737	the average nucleotide identity	707:737	the average nucleotide identity	707:737	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	47	theme	nucleotide	719:728	arg1	characteristics					798:812	phenotypic and biochemical characteristics	771:812	phenotypic and biochemical characteristics	771:812	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	47	theme	nucleotide	719:728	arg1	hybridization					756:768	digital DNA-DNA hybridization	740:768	digital DNA-DNA hybridization	740:768	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	9	48	theme	descriptions	1428:1439	arg1	emendations					1405:1415	the corresponding emendations	1387:1415	the corresponding emendations of species descriptions	1387:1439	Thus, the corresponding emendations of species descriptions are also proposed.					
31066660	5	49	theme	gen.	1012:1015	arg1	sediminis					839:847	Laceyella sediminis	829:847	Laceyella sediminis	829:847	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	49	theme	gen.	1012:1015	arg1	comb					1023:1026	comb	1023:1026	comb	1023:1026	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	49	theme	gen.	1012:1015	arg1	nov.					1017:1020	Paenactinomyces guangxiensis gen. nov.	983:1020	Paenactinomyces guangxiensis gen. nov.	983:1020	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	8	50	theme	genome	1189:1194	arg1	sequences					1196:1204	genome sequences	1189:1204	genome sequences	1189:1204	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	2	51	theme	gene	344:347	arg1	sequences					349:357	16S rRNA gene sequences	335:357	16S rRNA gene sequences	335:357	However, phylogenetic analysis merely based on 16S rRNA gene sequences cannot infer a robust and reliable phylogeny.					
31066660	9	52	theme	corresponding	1391:1403	arg1	emendations					1405:1415	the corresponding emendations	1387:1415	the corresponding emendations of species descriptions	1387:1439	Thus, the corresponding emendations of species descriptions are also proposed.					
31066660	2	53	theme	robust	374:379	arg1	phylogeny					394:402	a robust and reliable phylogeny	372:402	a robust and reliable phylogeny	372:402	However, phylogenetic analysis merely based on 16S rRNA gene sequences cannot infer a robust and reliable phylogeny.					
31066660	8	54	theme	better	1352:1357	arg1	fit					1359:1361	a significantly better fit	1336:1361	a significantly better fit to the phylogeny	1336:1378	In addition, compared to values calculated directly from genome sequences, the genomic DNA G+C contents mentioned in some species descriptions are too imprecise; and the corrected G+C content values have a significantly better fit to the phylogeny.					
31066660	5	55	theme	later	877:881	arg1	synonym					895:901	a later heterotypic synonym	875:901	a later heterotypic synonym of Laceyella tengchongensis	875:929	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	55	theme	later	877:881	arg1	sediminis					839:847	Laceyella sediminis	829:847	Laceyella sediminis	829:847	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	56	theme	tengchongensis	916:929	arg1	synonym					895:901	a later heterotypic synonym	875:901	a later heterotypic synonym of Laceyella tengchongensis	875:929	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	5	56	theme	tengchongensis	916:929	arg1	sediminis					839:847	Laceyella sediminis	829:847	Laceyella sediminis	829:847	In addition, based on the average nucleotide identity, digital DNA-DNA hybridization, phenotypic and biochemical characteristics, we found that Laceyella sediminis should be reclassified as a later heterotypic synonym of Laceyella tengchongensis; and reclassified Thermoactinomyces guangxiensis as Paenactinomyces guangxiensis gen. nov., comb.					
31066660	4	57	theme	rRNA	664:667	arg1	sequences					674:682	the 16S rRNA gene sequences	656:682	the 16S rRNA gene sequences	656:682	The topologies of phylogenomic trees are different from those of the 16S rRNA gene sequences.					
31066660	2	58	theme	phylogenetic	297:308	arg1	analysis					310:317	phylogenetic analysis	297:317	phylogenetic analysis merely based on 16S rRNA gene sequences	297:357	However, phylogenetic analysis merely based on 16S rRNA gene sequences cannot infer a robust and reliable phylogeny.					
31066660	1	59	theme	polyphasic	180:189	arg1	study					201:205	a polyphasic taxonomic study	178:205	a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics	178:285	The family Thermoactinomycetaceaecomprises 43 validly published species, which were identified by a polyphasic taxonomic study based on molecular phylogenetics, physiological and biochemical characteristics.					
31066660	4	60	theme	16S	660:662	arg1	sequences					674:682	the 16S rRNA gene sequences	656:682	the 16S rRNA gene sequences	656:682	The topologies of phylogenomic trees are different from those of the 16S rRNA gene sequences.					
30873704	6	0	theme	Paenibacillus	838:850	arg1	polymyxa					876:883	Paenibacillus polymyxa	862:883	Paenibacillus polymyxa	862:883	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	6	0	theme	Paenibacillus	838:850	arg1	cloacae					899:905	Enterobacter cloacae	886:905	Enterobacter cloacae	886:905	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	6	0	theme	Paenibacillus	838:850	arg1	coli					923:926	Escherichia coli	911:926	Escherichia coli	911:926	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	6	0	theme	Paenibacillus	838:850	arg1	polymyxa					852:859	Paenibacillus polymyxa	838:859	Paenibacillus polymyxa	838:859	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	13	1	dep	highest	1615:1621	arg1	NDF					1657:1659	NDF	1657:1659	NDF	1657:1659	The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively.					
30873704	13	1	dep	highest	1615:1621	arg1	digestibilities					1669:1683	ADF digestibilities	1665:1683	ADF digestibilities	1665:1683	The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively.					
30873704	13	1	dep	highest	1615:1621	arg1	CP					1653:1654	CP	1653:1654	CP	1653:1654	The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively.					
30873704	13	1	dep	highest	1615:1621	arg1	OM					1649:1650	OM	1649:1650	OM	1649:1650	The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively.					
30873704	13	1	dep	highest	1615:1621	arg1	DM					1645:1646	DM	1645:1646	DM	1645:1646	The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively.					
30873704	9	2	theme	lowest	1272:1277	arg1	NDF					1279:1281	NDF	1279:1281	NDF	1279:1281	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	15	3	theme	isolated	1996:2003	arg1	capable					2036:2042	capable	2036:2042	capable	2036:2042	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	15	3	theme	isolated	1996:2003	arg1	bacteria					2005:2012	the isolated bacteria	1992:2012	the isolated bacteria from horse faeces	1992:2030	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	15	4	dep	CONCLUSIONS	1931:1941	arg1	showed					1980:1985	showed	1980:1985	showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS	1980:2148	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	10	5	theme	ADF	1388:1390	arg1	values					1364:1369	The values	1360:1369	The values of NDF, iNDF and ADF	1360:1390	The values of NDF, iNDF and ADF were the highest and CP was the lowest in control.					
30873704	10	5	theme	ADF	1388:1390	arg1	highest					1401:1407	highest	1401:1407	highest	1401:1407	The values of NDF, iNDF and ADF were the highest and CP was the lowest in control.					
30873704	6	6	theme	sequence	748:755	arg1	analysis					757:764	16S rRNA sequence analysis	739:764	16S rRNA sequence analysis	739:764	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	16	7	theme	STUDY	2182:2186	arg1	straw					2194:2198	THE STUDY Wheat straw	2178:2198	THE STUDY Wheat straw	2178:2198	SIGNIFICANCE AND IMPACT OF THE STUDY Wheat straw is a major agro-residue fed to ruminants.					
30873704	8	8	theme	acid	1124:1127	arg1	contents					1151:1158	acid detergent fibre (ADF) contents	1124:1158	acid detergent fibre (ADF) contents of WS	1124:1164	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	10	9	theme	iNDF	1379:1382	arg1	values					1364:1369	The values	1360:1369	The values of NDF, iNDF and ADF	1360:1390	The values of NDF, iNDF and ADF were the highest and CP was the lowest in control.					
30873704	10	9	theme	iNDF	1379:1382	arg1	highest					1401:1407	highest	1401:1407	highest	1401:1407	The values of NDF, iNDF and ADF were the highest and CP was the lowest in control.					
30873704	6	10	theme	16S	739:741	arg1	analysis					757:764	16S rRNA sequence analysis	739:764	16S rRNA sequence analysis	739:764	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	3	11	theme	carboxymethyl	527:539	arg1	cellulose					541:549	carboxymethyl cellulose	527:549	carboxymethyl cellulose (CMC)	527:555	The cellulose-hydrolytic bacteria were isolated by using a medium amended with carboxymethyl cellulose (CMC).					
30873704	3	11	theme	carboxymethyl	527:539	arg1	CMC					552:554	CMC	552:554	CMC	552:554	The cellulose-hydrolytic bacteria were isolated by using a medium amended with carboxymethyl cellulose (CMC).					
30873704	4	12	from	CMC	642:644	arg1	release					610:616	the release	606:616	the release of reducing sugars from CMC	606:644	The activity of CMC was determined by measuring the release of reducing sugars from CMC.					
30873704	19	13	theme	gastrointestinal	2483:2498	arg1	tract					2500:2504	gastrointestinal tract	2483:2504	gastrointestinal tract of horse	2483:2513	The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.					
30873704	16	14	theme	straw	2194:2198	arg1	agro-residue					2211:2222	a major agro-residue	2203:2222	a major agro-residue fed to ruminants	2203:2239	SIGNIFICANCE AND IMPACT OF THE STUDY Wheat straw is a major agro-residue fed to ruminants.					
30873704	16	14	theme	straw	2194:2198	arg1	IMPACT					2168:2173	IMPACT	2168:2173	IMPACT	2168:2173	SIGNIFICANCE AND IMPACT OF THE STUDY Wheat straw is a major agro-residue fed to ruminants.					
30873704	16	14	theme	straw	2194:2198	arg1	SIGNIFICANCE					2151:2162	SIGNIFICANCE	2151:2162	SIGNIFICANCE	2151:2162	SIGNIFICANCE AND IMPACT OF THE STUDY Wheat straw is a major agro-residue fed to ruminants.					
30873704	8	15	theme	fibre	1139:1143	arg1	contents					1151:1158	acid detergent fibre (ADF) contents	1124:1158	acid detergent fibre (ADF) contents of WS	1124:1164	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	16	theme	indigestible	1096:1107	arg1	iNDF					1114:1117	iNDF	1114:1117	iNDF	1114:1117	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	16	theme	indigestible	1096:1107	arg1	NDF					1109:1111	indigestible NDF	1096:1111	indigestible NDF (iNDF)	1096:1118	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	17	theme	ADF	1146:1148	arg1	contents					1151:1158	acid detergent fibre (ADF) contents	1124:1158	acid detergent fibre (ADF) contents of WS	1124:1164	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	0	18	theme	effect	126:131	arg1	investigation					103:115	investigation	103:115	investigation of their effect on the nutritional value of wheat straw	103:171	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	0	18	theme	effect	126:131	arg1	tract					76:80	gastrointestinal tract	59:80	gastrointestinal tract of Arabian horse	59:97	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	9	19	theme	WS	1309:1310	arg1	disappearance					1228:1240	The highest disappearance	1216:1240	The highest disappearance of DM and CP contents	1216:1262	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	9	19	theme	WS	1309:1310	arg1	iNDF					1284:1287	iNDF	1284:1287	iNDF	1284:1287	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	9	19	theme	WS	1309:1310	arg1	contents					1297:1304	ADF contents	1293:1304	ADF contents	1293:1304	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	9	19	theme	WS	1309:1310	arg1	NDF					1279:1281	NDF	1279:1281	NDF	1279:1281	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	4	20	theme	sugars	630:635	arg1	release					610:616	the release	606:616	the release of reducing sugars from CMC	606:644	The activity of CMC was determined by measuring the release of reducing sugars from CMC.					
30873704	19	21	attach	isolated	2469:2476	arg2	bacteria					2460:2467	cellulolytic bacteria	2447:2467	cellulolytic bacteria isolated from gastrointestinal tract of horse	2447:2513	The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.					
30873704	19	21	attach	isolated	2469:2476	arg1	tract					2500:2504	gastrointestinal tract	2483:2504	gastrointestinal tract of horse	2483:2513	The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.					
30873704	0	22	from	tract	76:80	arg1	identification					14:27	identification	14:27	identification	14:27	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	0	22	from	tract	76:80	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	0	22	from	tract	76:80	arg1	value					152:156	the nutritional value	136:156	the nutritional value of wheat straw	136:171	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	8	23	theme	WS	1163:1164	arg1	protein					1051:1057	crude protein	1045:1057	crude protein (CP)	1045:1062	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	23	theme	WS	1163:1164	arg1	detergent					1073:1081	neutral detergent fibre	1065:1087	neutral detergent fibre (NDF)	1065:1093	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	23	theme	WS	1163:1164	arg1	matter					1029:1034	dry matter	1025:1034	dry matter (DM)	1025:1039	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	23	theme	WS	1163:1164	arg1	NDF					1109:1111	indigestible NDF	1096:1111	indigestible NDF (iNDF)	1096:1118	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	23	theme	WS	1163:1164	arg1	DM					1037:1038	DM	1037:1038	DM	1037:1038	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	23	theme	WS	1163:1164	arg1	iNDF					1114:1117	iNDF	1114:1117	iNDF	1114:1117	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	23	theme	WS	1163:1164	arg1	CP					1060:1061	CP	1060:1061	CP	1060:1061	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	23	theme	WS	1163:1164	arg1	NDF					1090:1092	NDF	1090:1092	NDF	1090:1092	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	23	theme	WS	1163:1164	arg1	contents					1151:1158	acid detergent fibre (ADF) contents	1124:1158	acid detergent fibre (ADF) contents of WS	1124:1164	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	14	24	theme	bacteria	1853:1860	arg1	b					1787:1787	b	1787:1787	b	1787:1787	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	14	24	theme	bacteria	1853:1860	arg1	TDOM					1822:1825	TDOM	1822:1825	TDOM	1822:1825	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	14	24	theme	bacteria	1853:1860	arg1	production					1775:1784	gas production	1771:1784	gas production (b)	1771:1788	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	14	24	theme	bacteria	1853:1860	arg1	biomass					1842:1848	microbial biomass	1832:1848	microbial biomass of bacteria	1832:1860	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	14	24	theme	bacteria	1853:1860	arg1	matter					1814:1819	truly degraded organic matter	1791:1819	truly degraded organic matter (TDOM)	1791:1826	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	19	25	theme	biological	2531:2540	arg1	treatment					2542:2550	biological treatment	2531:2550	biological treatment of WS	2531:2556	The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.					
30873704	12	26	theme	Bacterial	1525:1533	arg1	treatments					1535:1544	Bacterial treatments	1525:1544	Bacterial treatments	1525:1544	Bacterial treatments enhanced (P < 0·05) DM, OM, CP, NDF and ADF digestibility of WS.					
30873704	7	27	theme	isolated	977:984	arg1	bacteria					986:993	the isolated bacteria	973:993	the isolated bacteria in liquid medium	973:1010	Wheat straw was incubated with the isolated bacteria in liquid medium.					
30873704	9	28	theme	P.	1343:1344	arg1	L11					1355:1357	P. polymyxa L11	1343:1357	P. polymyxa L11	1343:1357	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	8	29	theme	microbial	1183:1191	arg1	P < 0·05					1205:1212	P < 0·05	1205:1212	P < 0·05	1205:1212	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	29	theme	microbial	1183:1191	arg1	treatments					1193:1202	microbial treatments	1183:1202	microbial treatments (P < 0·05)	1183:1213	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	13	30	theme	ADF	1665:1667	arg1	digestibilities					1669:1683	ADF digestibilities	1665:1683	ADF digestibilities	1665:1683	The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively.					
30873704	5	31	dep	isolates	672:679	arg1	L2					692:693	L2	692:693	L2	692:693	Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity.					
30873704	5	31	dep	isolates	672:679	arg1	L12					687:689	L12	687:689	L12	687:689	Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity.					
30873704	5	31	dep	isolates	672:679	arg1	L11					682:684	L11	682:684	L11	682:684	Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity.					
30873704	5	31	dep	isolates	672:679	arg1	isolates					672:679	four isolates	667:679	four isolates (L11, L12, L2 and Z2)	667:701	Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity.					
30873704	5	31	dep	isolates	672:679	arg1	Z2					699:700	Z2	699:700	Z2	699:700	Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity.					
30873704	0	32	theme	wheat	161:165	arg1	straw					167:171	wheat straw	161:171	wheat straw	161:171	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	0	33	theme	Arabian	85:91	arg1	horse					93:97	Arabian horse	85:97	Arabian horse	85:97	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	19	34	used	used	2522:2525	arg2	bacteria					2460:2467	cellulolytic bacteria	2447:2467	cellulolytic bacteria isolated from gastrointestinal tract of horse	2447:2513	The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.					
30873704	14	35	theme	microbial	1832:1840	arg1	biomass					1842:1848	microbial biomass	1832:1848	microbial biomass of bacteria	1832:1860	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	17	36	theme	high	2251:2254	arg1	contents					2262:2269	high fibre contents	2251:2269	high fibre contents	2251:2269	However, high fibre contents reduce digestibility and limit animal productivity.					
30873704	1	37	theme	wheat	356:360	arg1	straw					362:366	wheat straw	356:366	wheat straw (WS)	356:371	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	1	37	theme	wheat	356:360	arg1	WS					369:370	WS	369:370	WS	369:370	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	4	38	theme	CMC	574:576	arg1	activity					562:569	The activity	558:569	The activity of CMC	558:576	The activity of CMC was determined by measuring the release of reducing sugars from CMC.					
30873704	0	39	theme	bacteria	45:52	arg1	identification					14:27	identification	14:27	identification	14:27	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	0	39	theme	bacteria	45:52	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	2	40	theme	Arabian	432:438	arg1	horses					440:445	four Arabian horses	427:445	four Arabian horses	427:445	METHODS AND RESULTS Fresh faeces were collected from four Arabian horses.					
30873704	18	41	theme	ruminal	2352:2358	arg1	degradation					2360:2370	ruminal degradation	2352:2370	ruminal degradation of these by-products	2352:2391	It seems that enhancement of ruminal degradation of these by-products is necessary.					
30873704	14	42	theme	organic	1806:1812	arg1	TDOM					1822:1825	TDOM	1822:1825	TDOM	1822:1825	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	14	42	theme	organic	1806:1812	arg1	matter					1814:1819	truly degraded organic matter	1791:1819	truly degraded organic matter (TDOM)	1791:1826	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	1	43	theme	horse	293:297	arg1	tract					276:280	gastrointestinal tract	259:280	gastrointestinal tract of Arabian horse	259:297	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	8	44	theme	dry	1025:1027	arg1	DM					1037:1038	DM	1037:1038	DM	1037:1038	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	44	theme	dry	1025:1027	arg1	matter					1029:1034	dry matter	1025:1034	dry matter (DM)	1025:1039	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	16	45	theme	major	2205:2209	arg1	agro-residue					2211:2222	a major agro-residue	2203:2222	a major agro-residue fed to ruminants	2203:2239	SIGNIFICANCE AND IMPACT OF THE STUDY Wheat straw is a major agro-residue fed to ruminants.					
30873704	16	45	theme	major	2205:2209	arg1	IMPACT					2168:2173	IMPACT	2168:2173	IMPACT	2168:2173	SIGNIFICANCE AND IMPACT OF THE STUDY Wheat straw is a major agro-residue fed to ruminants.					
30873704	16	45	theme	major	2205:2209	arg1	SIGNIFICANCE					2151:2162	SIGNIFICANCE	2151:2162	SIGNIFICANCE	2151:2162	SIGNIFICANCE AND IMPACT OF THE STUDY Wheat straw is a major agro-residue fed to ruminants.					
30873704	19	46	theme	WS	2555:2556	arg1	treatment					2542:2550	biological treatment	2531:2550	biological treatment of WS	2531:2556	The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.					
30873704	18	47	theme	by-products	2381:2391	arg1	degradation					2360:2370	ruminal degradation	2352:2370	ruminal degradation of these by-products	2352:2391	It seems that enhancement of ruminal degradation of these by-products is necessary.					
30873704	2	48	theme	Fresh	394:398	arg1	faeces					400:405	METHODS AND RESULTS Fresh faeces	374:405	METHODS AND RESULTS Fresh faeces	374:405	METHODS AND RESULTS Fresh faeces were collected from four Arabian horses.					
30873704	0	49	theme	cellulolytic	32:43	arg1	bacteria					45:52	cellulolytic bacteria	32:52	cellulolytic bacteria	32:52	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	9	50	theme	highest	1220:1226	arg1	disappearance					1228:1240	The highest disappearance	1216:1240	The highest disappearance of DM and CP contents	1216:1262	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	11	51	theme	samples	1500:1506	arg1	digestibility					1473:1485	digestibility	1473:1485	digestibility of processed samples	1473:1506	Gas production parameters and digestibility of processed samples were determined.					
30873704	11	51	theme	samples	1500:1506	arg1	parameters					1458:1467	Gas production parameters	1443:1467	Gas production parameters	1443:1467	Gas production parameters and digestibility of processed samples were determined.					
30873704	6	52	with	similarity	822:831	arg1	polymyxa					876:883	Paenibacillus polymyxa	862:883	Paenibacillus polymyxa	862:883	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	6	52	with	similarity	822:831	arg1	cloacae					899:905	Enterobacter cloacae	886:905	Enterobacter cloacae	886:905	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	6	52	with	similarity	822:831	arg1	coli					923:926	Escherichia coli	911:926	Escherichia coli	911:926	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	6	52	with	similarity	822:831	arg1	polymyxa					852:859	Paenibacillus polymyxa	838:859	Paenibacillus polymyxa	838:859	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	12	53	dep	enhanced	1546:1553	arg1	P < 0·05					1556:1563	P < 0·05	1556:1563	P < 0·05	1556:1563	Bacterial treatments enhanced (P < 0·05) DM, OM, CP, NDF and ADF digestibility of WS.					
30873704	8	54	theme	crude	1045:1049	arg1	CP					1060:1061	CP	1060:1061	CP	1060:1061	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	54	theme	crude	1045:1049	arg1	protein					1051:1057	crude protein	1045:1057	crude protein (CP)	1045:1062	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	19	55	theme	cellulolytic	2447:2458	arg1	bacteria					2460:2467	cellulolytic bacteria	2447:2467	cellulolytic bacteria isolated from gastrointestinal tract of horse	2447:2513	The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.					
30873704	8	56	dep	Disappeared	1013:1023	arg1	affected					1171:1178	affected	1171:1178	were affected by microbial treatments (P < 0·05)	1166:1213	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	9	57	theme	contents	1255:1262	arg1	disappearance					1228:1240	The highest disappearance	1216:1240	The highest disappearance of DM and CP contents	1216:1262	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	9	57	theme	contents	1255:1262	arg1	iNDF					1284:1287	iNDF	1284:1287	iNDF	1284:1287	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	9	57	theme	contents	1255:1262	arg1	contents					1297:1304	ADF contents	1293:1304	ADF contents	1293:1304	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	9	57	theme	contents	1255:1262	arg1	NDF					1279:1281	NDF	1279:1281	NDF	1279:1281	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	12	58	theme	ADF	1586:1588	arg1	digestibility					1590:1602	ADF digestibility	1586:1602	ADF digestibility	1586:1602	Bacterial treatments enhanced (P < 0·05) DM, OM, CP, NDF and ADF digestibility of WS.					
30873704	0	59	from	effect	126:131	arg1	value					152:156	the nutritional value	136:156	the nutritional value of wheat straw	136:171	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	11	60	theme	production	1447:1456	arg1	parameters					1458:1467	Gas production parameters	1443:1467	Gas production parameters	1443:1467	Gas production parameters and digestibility of processed samples were determined.					
30873704	1	61	from	effect	321:326	arg1	value					347:351	the nutritional value	331:351	the nutritional value of wheat straw (WS)	331:371	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	15	62	theme	horse	2019:2023	arg1	faeces					2025:2030	horse faeces	2019:2030	horse faeces	2019:2030	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	6	63	dep	similarity	822:831	arg1	%					820:820	%	820:820	%	820:820	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	1	64	theme	gastrointestinal	259:274	arg1	tract					276:280	gastrointestinal tract	259:280	gastrointestinal tract of Arabian horse	259:297	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	8	65	theme	neutral	1065:1071	arg1	detergent					1073:1081	neutral detergent fibre	1065:1087	neutral detergent fibre (NDF)	1065:1093	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	65	theme	neutral	1065:1071	arg1	NDF					1090:1092	NDF	1090:1092	NDF	1090:1092	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	16	66	theme	Wheat	2188:2192	arg1	straw					2194:2198	THE STUDY Wheat straw	2178:2198	THE STUDY Wheat straw	2178:2198	SIGNIFICANCE AND IMPACT OF THE STUDY Wheat straw is a major agro-residue fed to ruminants.					
30873704	0	67	from	value	152:156	arg1	investigation					103:115	investigation	103:115	investigation of their effect on the nutritional value of wheat straw	103:171	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	0	67	from	value	152:156	arg1	tract					76:80	gastrointestinal tract	59:80	gastrointestinal tract of Arabian horse	59:97	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	6	68	theme	rRNA	743:746	arg1	analysis					757:764	16S rRNA sequence analysis	739:764	16S rRNA sequence analysis	739:764	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	8	69	theme	fibre	1083:1087	arg1	detergent					1073:1081	neutral detergent fibre	1065:1087	neutral detergent fibre (NDF)	1065:1093	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	8	69	theme	fibre	1083:1087	arg1	NDF					1090:1092	NDF	1090:1092	NDF	1090:1092	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	5	70	theme	maximum	710:716	arg1	capacity					729:736	maximum hydrolysis capacity	710:736	maximum hydrolysis capacity	710:736	Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity.					
30873704	9	71	theme	ADF	1293:1295	arg1	contents					1297:1304	ADF contents	1293:1304	ADF contents	1293:1304	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	8	72	theme	detergent	1129:1137	arg1	contents					1151:1158	acid detergent fibre (ADF) contents	1124:1158	acid detergent fibre (ADF) contents of WS	1124:1164	Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05).					
30873704	19	73	theme	horse	2509:2513	arg1	tract					2500:2504	gastrointestinal tract	2483:2504	gastrointestinal tract of horse	2483:2513	The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.					
30873704	7	74	theme	liquid	998:1003	arg1	medium					1005:1010	liquid medium	998:1010	liquid medium	998:1010	Wheat straw was incubated with the isolated bacteria in liquid medium.					
30873704	15	75	from	faeces	2025:2030	arg1	capable					2036:2042	capable	2036:2042	capable	2036:2042	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	15	75	from	faeces	2025:2030	arg1	bacteria					2005:2012	the isolated bacteria	1992:2012	the isolated bacteria from horse faeces	1992:2030	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	17	76	theme	animal	2302:2307	arg1	productivity					2309:2320	animal productivity	2302:2320	animal productivity	2302:2320	However, high fibre contents reduce digestibility and limit animal productivity.					
30873704	4	77	theme	reducing	621:628	arg1	sugars					630:635	reducing sugars	621:635	reducing sugars	621:635	The activity of CMC was determined by measuring the release of reducing sugars from CMC.					
30873704	6	78	theme	Paenibacillus	862:874	arg1	polymyxa					876:883	Paenibacillus polymyxa	862:883	Paenibacillus polymyxa	862:883	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	6	78	theme	Paenibacillus	862:874	arg1	polymyxa					852:859	Paenibacillus polymyxa	838:859	Paenibacillus polymyxa	838:859	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	0	79	theme	straw	167:171	arg1	value					152:156	the nutritional value	136:156	the nutritional value of wheat straw	136:171	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	13	80	dep	isolates	1719:1726	arg1	control					1736:1742	control	1736:1742	control	1736:1742	The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively.					
30873704	13	80	dep	isolates	1719:1726	arg1	L12					1728:1730	L12	1728:1730	L12	1728:1730	The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively.					
30873704	13	80	dep	isolates	1719:1726	arg1	isolates					1719:1726	isolates L12 and control	1719:1742	isolates L12 and control	1719:1742	The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively.					
30873704	0	81	from	investigation	103:115	arg1	identification					14:27	identification	14:27	identification	14:27	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	0	81	from	investigation	103:115	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	0	81	from	investigation	103:115	arg1	value					152:156	the nutritional value	136:156	the nutritional value of wheat straw	136:171	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	15	82	theme	chemical	2060:2067	arg1	composition					2069:2079	the chemical composition	2056:2079	the chemical composition	2056:2079	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	7	83	theme	Wheat	942:946	arg1	straw					948:952	Wheat straw	942:952	Wheat straw	942:952	Wheat straw was incubated with the isolated bacteria in liquid medium.					
30873704	9	84	theme	polymyxa	1346:1353	arg1	L11					1355:1357	P. polymyxa L11	1343:1357	P. polymyxa L11	1343:1357	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	0	85	theme	horse	93:97	arg1	investigation					103:115	investigation	103:115	investigation of their effect on the nutritional value of wheat straw	103:171	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	0	85	theme	horse	93:97	arg1	tract					76:80	gastrointestinal tract	59:80	gastrointestinal tract of Arabian horse	59:97	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	1	86	theme	nutritional	335:345	arg1	value					347:351	the nutritional value	331:351	the nutritional value of wheat straw (WS)	331:371	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	15	87	theme	nutrition	2128:2136	arg1	value					2138:2142	nutrition value	2128:2142	nutrition value of WS	2128:2148	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	1	88	from	tract	276:280	arg1	bacteria					245:252	the cellulolytic bacteria	228:252	the cellulolytic bacteria from gastrointestinal tract of Arabian horse	228:297	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	14	89	theme	biomass	1842:1848	arg1	Potential					1758:1766	Potential	1758:1766	Potential	1758:1766	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	6	90	contain	possessed	793:801	arg1	isolates					784:791	these isolates	778:791	these isolates	778:791	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	6	90	contain	possessed	793:801	arg2	similarity					822:831	99, 99, 99 and 98% similarity	803:831	99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively	803:939	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	15	91	theme	WS	2147:2148	arg1	value					2138:2142	nutrition value	2128:2142	nutrition value of WS	2128:2148	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	14	92	theme	gas	1771:1773	arg1	b					1787:1787	b	1787:1787	b	1787:1787	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	14	92	theme	gas	1771:1773	arg1	production					1775:1784	gas production	1771:1784	gas production (b)	1771:1788	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	15	93	dep	composition	2069:2079	arg1	enhancing					2118:2126	enhancing	2118:2126	increasing digestibility as well as enhancing nutrition value of WS	2082:2148	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	15	93	dep	composition	2069:2079	arg1	increasing					2082:2091	increasing	2082:2091	increasing digestibility as well as enhancing nutrition value of WS	2082:2148	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	17	94	theme	fibre	2256:2260	arg1	contents					2262:2269	high fibre contents	2251:2269	high fibre contents	2251:2269	However, high fibre contents reduce digestibility and limit animal productivity.					
30873704	1	95	theme	straw	362:366	arg1	value					347:351	the nutritional value	331:351	the nutritional value of wheat straw (WS)	331:371	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	14	96	theme	Potential	1758:1766	arg1	treatments					1862:1871	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments	1758:1871	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments	1758:1871	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	18	97	theme	degradation	2360:2370	arg1	enhancement					2337:2347	enhancement	2337:2347	enhancement of ruminal degradation of these by-products	2337:2391	It seems that enhancement of ruminal degradation of these by-products is necessary.					
30873704	2	98	theme	METHODS	374:380	arg1	faeces					400:405	METHODS AND RESULTS Fresh faeces	374:405	METHODS AND RESULTS Fresh faeces	374:405	METHODS AND RESULTS Fresh faeces were collected from four Arabian horses.					
30873704	0	99	theme	nutritional	140:150	arg1	value					152:156	the nutritional value	136:156	the nutritional value of wheat straw	136:171	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	14	100	theme	matter	1814:1819	arg1	Potential					1758:1766	Potential	1758:1766	Potential	1758:1766	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	1	101	theme	Arabian	285:291	arg1	horse					293:297	Arabian horse	285:297	Arabian horse	285:297	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	14	102	theme	degraded	1797:1804	arg1	TDOM					1822:1825	TDOM	1822:1825	TDOM	1822:1825	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	14	102	theme	degraded	1797:1804	arg1	matter					1814:1819	truly degraded organic matter	1791:1819	truly degraded organic matter (TDOM)	1791:1826	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	3	103	theme	cellulose-hydrolytic	452:471	arg1	bacteria					473:480	The cellulose-hydrolytic bacteria	448:480	The cellulose-hydrolytic bacteria	448:480	The cellulose-hydrolytic bacteria were isolated by using a medium amended with carboxymethyl cellulose (CMC).					
30873704	5	104	theme	hydrolysis	718:727	arg1	capacity					729:736	maximum hydrolysis capacity	710:736	maximum hydrolysis capacity	710:736	Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity.					
30873704	14	105	dep	higher	1892:1897	arg1	compared					1910:1917	compared	1910:1917	compared to control	1910:1928	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	14	105	dep	higher	1892:1897	arg1	P < 0·05					1900:1907	P < 0·05	1900:1907	P < 0·05	1900:1907	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	11	106	theme	processed	1490:1498	arg1	samples					1500:1506	processed samples	1490:1506	processed samples	1490:1506	Gas production parameters and digestibility of processed samples were determined.					
30873704	6	107	dep	%	820:820	arg1	98					818:819	98	818:819	98	818:819	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	6	107	dep	%	820:820	arg1	99					811:812	99	811:812	99	811:812	16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively.					
30873704	2	108	theme	RESULTS	386:392	arg1	faeces					400:405	METHODS AND RESULTS Fresh faeces	374:405	METHODS AND RESULTS Fresh faeces	374:405	METHODS AND RESULTS Fresh faeces were collected from four Arabian horses.					
30873704	0	109	theme	gastrointestinal	59:74	arg1	tract					76:80	gastrointestinal tract	59:80	gastrointestinal tract of Arabian horse	59:97	Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.					
30873704	15	110	theme	study	1974:1978	arg1	results					1958:1964	the results	1954:1964	the results of this study	1954:1978	CONCLUSIONS Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS.					
30873704	1	111	dep	AIMS	174:177	arg1	conducted					194:202	conducted	194:202	was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS)	190:371	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
30873704	9	112	theme	DM	1245:1246	arg1	contents					1255:1262	DM and CP contents	1245:1262	DM and CP contents	1245:1262	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	12	113	theme	WS	1607:1608	arg1	OM					1570:1571	OM	1570:1571	OM	1570:1571	Bacterial treatments enhanced (P < 0·05) DM, OM, CP, NDF and ADF digestibility of WS.					
30873704	12	113	theme	WS	1607:1608	arg1	DM					1566:1567	DM	1566:1567	DM	1566:1567	Bacterial treatments enhanced (P < 0·05) DM, OM, CP, NDF and ADF digestibility of WS.					
30873704	12	113	theme	WS	1607:1608	arg1	NDF					1578:1580	NDF	1578:1580	NDF	1578:1580	Bacterial treatments enhanced (P < 0·05) DM, OM, CP, NDF and ADF digestibility of WS.					
30873704	12	113	theme	WS	1607:1608	arg1	digestibility					1590:1602	ADF digestibility	1586:1602	ADF digestibility	1586:1602	Bacterial treatments enhanced (P < 0·05) DM, OM, CP, NDF and ADF digestibility of WS.					
30873704	12	113	theme	WS	1607:1608	arg1	CP					1574:1575	CP	1574:1575	CP	1574:1575	Bacterial treatments enhanced (P < 0·05) DM, OM, CP, NDF and ADF digestibility of WS.					
30873704	14	114	theme	production	1775:1784	arg1	Potential					1758:1766	Potential	1758:1766	Potential	1758:1766	Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control.					
30873704	9	115	theme	CP	1252:1253	arg1	contents					1255:1262	DM and CP contents	1245:1262	DM and CP contents	1245:1262	The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11.					
30873704	19	116	theme	study	2427:2431	arg1	results					2411:2417	The results	2407:2417	The results of this study	2407:2431	The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.					
30873704	7	117	from	bacteria	986:993	arg1	medium					1005:1010	liquid medium	998:1010	liquid medium	998:1010	Wheat straw was incubated with the isolated bacteria in liquid medium.					
30873704	10	118	theme	NDF	1374:1376	arg1	values					1364:1369	The values	1360:1369	The values of NDF, iNDF and ADF	1360:1390	The values of NDF, iNDF and ADF were the highest and CP was the lowest in control.					
30873704	10	118	theme	NDF	1374:1376	arg1	highest					1401:1407	highest	1401:1407	highest	1401:1407	The values of NDF, iNDF and ADF were the highest and CP was the lowest in control.					
30873704	11	119	theme	Gas	1443:1445	arg1	parameters					1458:1467	Gas production parameters	1443:1467	Gas production parameters	1443:1467	Gas production parameters and digestibility of processed samples were determined.					
30873704	1	120	theme	cellulolytic	232:243	arg1	bacteria					245:252	the cellulolytic bacteria	228:252	the cellulolytic bacteria from gastrointestinal tract of Arabian horse	228:297	AIMS This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS).					
29378275	2	0	theme	using	359:363	arg1	SEM					377:379	SEM	377:379	SEM	377:379	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	2	0	theme	using	359:363	arg1	XRD					372:374	XRD	372:374	XRD	372:374	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	2	0	theme	using	359:363	arg1	FT-IR					365:369	the adsorbent using FT-IR	345:369	the adsorbent using FT-IR	345:369	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	6	1	theme	∆H°	896:898	arg1	values					873:878	The experimental values	856:878	The experimental values of ∆G°, ∆S° and ∆H°	856:898	The experimental values of ∆G°, ∆S° and ∆H° revealed that the nature of adsorption was feasible, spontaneous and exothermic.					
29378275	1	2	from	solution	315:322	arg1	removal					276:282	the effective removal	262:282	the effective removal of phosphate ions from aqueous solution	262:322	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	2	3	theme	adsorbent	349:357	arg1	SEM					377:379	SEM	377:379	SEM	377:379	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	2	3	theme	adsorbent	349:357	arg1	XRD					372:374	XRD	372:374	XRD	372:374	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	2	3	theme	adsorbent	349:357	arg1	FT-IR					365:369	the adsorbent using FT-IR	345:369	the adsorbent using FT-IR	345:369	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	0	4	theme	ions	104:107	arg1	removal					83:89	the adsorptive removal	68:89	the adsorptive removal of phosphate ions from aqueous solution	68:129	Lanthanum (III) encapsulated chitosan-montmorillonite composite for the adsorptive removal of phosphate ions from aqueous solution.					
29378275	3	5	theme	operating	513:521	arg1	parameters					523:532	various operating parameters	505:532	various operating parameters	505:532	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	0	6	theme	phosphate	94:102	arg1	ions					104:107	phosphate ions	94:107	phosphate ions	94:107	Lanthanum (III) encapsulated chitosan-montmorillonite composite for the adsorptive removal of phosphate ions from aqueous solution.					
29378275	6	7	theme	∆S°	888:890	arg1	values					873:878	The experimental values	856:878	The experimental values of ∆G°, ∆S° and ∆H°	856:898	The experimental values of ∆G°, ∆S° and ∆H° revealed that the nature of adsorption was feasible, spontaneous and exothermic.					
29378275	3	8	theme	parameters	523:532	arg1	effects					494:500	the effects	490:500	the effects of various operating parameters	490:532	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	0	9	theme	aqueous	114:120	arg1	solution					122:129	aqueous solution	114:129	aqueous solution	114:129	Lanthanum (III) encapsulated chitosan-montmorillonite composite for the adsorptive removal of phosphate ions from aqueous solution.					
29378275	5	10	theme	equilibrium	829:839	arg1	data					850:853	the equilibrium isotherm data	825:853	the equilibrium isotherm data	825:853	Freundlich isotherm model fit well with the equilibrium isotherm data.					
29378275	2	11	theme	TGA	391:393	arg1	techniques					403:412	EDX, TGA and DTA techniques	386:412	EDX, TGA and DTA techniques	386:412	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	8	12	theme	efficiency	1173:1182	arg1	efficiency					1173:1182	adsorption efficiency	1162:1182	adsorption efficiency	1162:1182	Regeneration study of the spent La-CS-MMT-P composite was carried out upto five cycles and found that about 70% of adsorption efficiency was retained.					
29378275	8	12	theme	efficiency	1173:1182	arg1	%					1157:1157	about 70%	1149:1157	about 70% of adsorption efficiency	1149:1182	Regeneration study of the spent La-CS-MMT-P composite was carried out upto five cycles and found that about 70% of adsorption efficiency was retained.					
29378275	6	13	theme	∆G°	883:885	arg1	values					873:878	The experimental values	856:878	The experimental values of ∆G°, ∆S° and ∆H°	856:898	The experimental values of ∆G°, ∆S° and ∆H° revealed that the nature of adsorption was feasible, spontaneous and exothermic.					
29378275	3	14	theme	various	505:511	arg1	parameters					523:532	various operating parameters	505:532	various operating parameters	505:532	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	8	15	theme	adsorption	1162:1171	arg1	efficiency					1173:1182	adsorption efficiency	1162:1182	adsorption efficiency	1162:1182	Regeneration study of the spent La-CS-MMT-P composite was carried out upto five cycles and found that about 70% of adsorption efficiency was retained.					
29378275	4	16	theme	hydrogen	719:726	arg1	bonding					728:734	hydrogen bonding	719:734	hydrogen bonding	719:734	Phosphate ions adsorbed effectively on La-CS-MMT composite through hydrogen bonding and by outer-sphere complex formation mechanism.					
29378275	1	17	theme	effective	266:274	arg1	removal					276:282	the effective removal	262:282	the effective removal of phosphate ions from aqueous solution	262:322	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	8	18	theme	Regeneration	1047:1058	arg1	study					1060:1064	Regeneration study	1047:1064	Regeneration study of the spent La-CS-MMT-P composite	1047:1099	Regeneration study of the spent La-CS-MMT-P composite was carried out upto five cycles and found that about 70% of adsorption efficiency was retained.					
29378275	1	19	theme	encapsulated	169:180	arg1	adsorbent					248:256	adsorbent	248:256	adsorbent	248:256	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	1	19	theme	encapsulated	169:180	arg1	La-CS-MMT					218:226	lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT)	153:227	lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT)	153:227	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	8	20	theme	spent	1073:1077	arg1	composite					1091:1099	the spent La-CS-MMT-P composite	1069:1099	the spent La-CS-MMT-P composite	1069:1099	Regeneration study of the spent La-CS-MMT-P composite was carried out upto five cycles and found that about 70% of adsorption efficiency was retained.					
29378275	1	21	theme	chitosan-montmorillonite	182:205	arg1	adsorbent					248:256	adsorbent	248:256	adsorbent	248:256	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	1	21	theme	chitosan-montmorillonite	182:205	arg1	La-CS-MMT					218:226	lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT)	153:227	lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT)	153:227	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	2	22	theme	DTA	399:401	arg1	techniques					403:412	EDX, TGA and DTA techniques	386:412	EDX, TGA and DTA techniques	386:412	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	3	23	theme	co-existing	614:624	arg1	ions					626:629	competitor co-existing ions	603:629	competitor co-existing ions	603:629	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	1	24	theme	lanthanum	153:161	arg1	adsorbent					248:256	adsorbent	248:256	adsorbent	248:256	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	1	24	theme	lanthanum	153:161	arg1	La-CS-MMT					218:226	lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT)	153:227	lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT)	153:227	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	1	25	theme	composite	207:215	arg1	adsorbent					248:256	adsorbent	248:256	adsorbent	248:256	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	1	25	theme	composite	207:215	arg1	La-CS-MMT					218:226	lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT)	153:227	lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT)	153:227	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	0	26	theme	chitosan-montmorillonite	29:52	arg1	composite					54:62	chitosan-montmorillonite composite	29:62	chitosan-montmorillonite composite	29:62	Lanthanum (III) encapsulated chitosan-montmorillonite composite for the adsorptive removal of phosphate ions from aqueous solution.					
29378275	1	27	theme	phosphate	287:295	arg1	ions					297:300	phosphate ions	287:300	phosphate ions	287:300	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	8	28	theme	composite	1091:1099	arg1	study					1060:1064	Regeneration study	1047:1064	Regeneration study of the spent La-CS-MMT-P composite	1047:1099	Regeneration study of the spent La-CS-MMT-P composite was carried out upto five cycles and found that about 70% of adsorption efficiency was retained.					
29378275	6	29	theme	experimental	860:871	arg1	values					873:878	The experimental values	856:878	The experimental values of ∆G°, ∆S° and ∆H°	856:898	The experimental values of ∆G°, ∆S° and ∆H° revealed that the nature of adsorption was feasible, spontaneous and exothermic.					
29378275	5	30	theme	isotherm	841:848	arg1	data					850:853	the equilibrium isotherm data	825:853	the equilibrium isotherm data	825:853	Freundlich isotherm model fit well with the equilibrium isotherm data.					
29378275	4	31	theme	Phosphate	652:660	arg1	ions					662:665	Phosphate ions	652:665	Phosphate ions	652:665	Phosphate ions adsorbed effectively on La-CS-MMT composite through hydrogen bonding and by outer-sphere complex formation mechanism.					
29378275	1	32	theme	ions	297:300	arg1	removal					276:282	the effective removal	262:282	the effective removal of phosphate ions from aqueous solution	262:322	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	8	33	theme	La-CS-MMT-P	1079:1089	arg1	composite					1091:1099	the spent La-CS-MMT-P composite	1069:1099	the spent La-CS-MMT-P composite	1069:1099	Regeneration study of the spent La-CS-MMT-P composite was carried out upto five cycles and found that about 70% of adsorption efficiency was retained.					
29378275	2	34	theme	EDX	386:388	arg1	techniques					403:412	EDX, TGA and DTA techniques	386:412	EDX, TGA and DTA techniques	386:412	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	5	35	theme	Freundlich	785:794	arg1	model					805:809	Freundlich isotherm model	785:809	Freundlich isotherm model	785:809	Freundlich isotherm model fit well with the equilibrium isotherm data.					
29378275	6	36	theme	adsorption	928:937	arg1	feasible					943:950	feasible	943:950	feasible	943:950	The experimental values of ∆G°, ∆S° and ∆H° revealed that the nature of adsorption was feasible, spontaneous and exothermic.					
29378275	6	36	theme	adsorption	928:937	arg1	nature					918:923	the nature	914:923	the nature of adsorption	914:937	The experimental values of ∆G°, ∆S° and ∆H° revealed that the nature of adsorption was feasible, spontaneous and exothermic.					
29378275	7	37	theme	pseudo-second-order	1020:1038	arg1	model					1040:1044	pseudo-second-order model	1020:1044	pseudo-second-order model	1020:1044	The kinetic study was well fitted with pseudo-second-order model.					
29378275	3	38	theme	competitor	603:612	arg1	ions					626:629	competitor co-existing ions	603:629	competitor co-existing ions	603:629	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	3	39	theme	adsorption	438:447	arg1	experiments					449:459	Batch adsorption experiments	432:459	Batch adsorption experiments	432:459	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	5	40	theme	isotherm	796:803	arg1	model					805:809	Freundlich isotherm model	785:809	Freundlich isotherm model	785:809	Freundlich isotherm model fit well with the equilibrium isotherm data.					
29378275	1	41	theme	aqueous	307:313	arg1	solution					315:322	aqueous solution	307:322	aqueous solution	307:322	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	3	42	theme	initial	554:560	arg1	concentration					562:574	initial concentration	554:574	initial concentration of phosphate ions	554:592	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	7	43	theme	kinetic	985:991	arg1	study					993:997	The kinetic study	981:997	The kinetic study	981:997	The kinetic study was well fitted with pseudo-second-order model.					
29378275	4	44	theme	complex	756:762	arg1	formation					764:772	outer-sphere complex formation	743:772	outer-sphere complex formation	743:772	Phosphate ions adsorbed effectively on La-CS-MMT composite through hydrogen bonding and by outer-sphere complex formation mechanism.					
29378275	4	45	theme	outer-sphere	743:754	arg1	formation					764:772	outer-sphere complex formation	743:772	outer-sphere complex formation	743:772	Phosphate ions adsorbed effectively on La-CS-MMT composite through hydrogen bonding and by outer-sphere complex formation mechanism.					
29378275	4	46	theme	La-CS-MMT	691:699	arg1	composite					701:709	La-CS-MMT composite	691:709	La-CS-MMT composite	691:709	Phosphate ions adsorbed effectively on La-CS-MMT composite through hydrogen bonding and by outer-sphere complex formation mechanism.					
29378275	0	47	theme	adsorptive	72:81	arg1	removal					83:89	the adsorptive removal	68:89	the adsorptive removal of phosphate ions from aqueous solution	68:129	Lanthanum (III) encapsulated chitosan-montmorillonite composite for the adsorptive removal of phosphate ions from aqueous solution.					
29378275	2	48	with	FT-IR	365:369	arg1	techniques					403:412	EDX, TGA and DTA techniques	386:412	EDX, TGA and DTA techniques	386:412	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	3	49	theme	phosphate	579:587	arg1	ions					589:592	phosphate ions	579:592	phosphate ions	579:592	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	3	50	theme	Batch	432:436	arg1	experiments					449:459	Batch adsorption experiments	432:459	Batch adsorption experiments	432:459	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	1	51	theme	present	139:145	arg1	work					147:150	the present work	135:150	the present work	135:150	In the present work, lanthanum (III) encapsulated chitosan-montmorillonite composite (La-CS-MMT) was prepared as an adsorbent for the effective removal of phosphate ions from aqueous solution.					
29378275	3	52	theme	ions	589:592	arg1	concentration					562:574	initial concentration	554:574	initial concentration of phosphate ions	554:592	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	3	52	theme	ions	589:592	arg1	ions					626:629	competitor co-existing ions	603:629	competitor co-existing ions	603:629	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	3	52	theme	ions	589:592	arg1	time					548:551	time	548:551	time	548:551	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	3	52	theme	ions	589:592	arg1	dosage					595:600	dosage	595:600	dosage	595:600	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	3	52	theme	ions	589:592	arg1	pH					632:633	pH	632:633	pH	632:633	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	3	52	theme	ions	589:592	arg1	temperature					639:649	temperature	639:649	temperature	639:649	Batch adsorption experiments were carried out to optimize the effects of various operating parameters viz., shaking time, initial concentration of phosphate ions, dosage, competitor co-existing ions, pH and temperature.					
29378275	2	53	theme	FT-IR	365:369	arg1	Characterization					325:340	Characterization	325:340	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques	325:412	Characterization of the adsorbent using FT-IR, XRD, SEM with EDX, TGA and DTA techniques were carried out.					
29378275	0	54	from	solution	122:129	arg1	removal					83:89	the adsorptive removal	68:89	the adsorptive removal of phosphate ions from aqueous solution	68:129	Lanthanum (III) encapsulated chitosan-montmorillonite composite for the adsorptive removal of phosphate ions from aqueous solution.					
30844259	0	0	theme	Azotobacter	80:90	arg1	vinelandii					92:101	Azotobacter vinelandii	80:101	Azotobacter vinelandii	80:101	Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii.					
30844259	0	1	from	Function	17:24	arg1	Alginate					59:66	Alginate	59:66	Alginate	59:66	Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii.					
30844259	3	2	from	influence	685:693	arg1	properties					706:715	gelling properties	698:715	gelling properties	698:715	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	6	3	theme	extracellular	1208:1220	arg1	epimerases					1233:1242	seven different extracellular mannuronan epimerases	1192:1242	seven different extracellular mannuronan epimerases (AlgE1-AlgE7)	1192:1256	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	6	3	theme	extracellular	1208:1220	arg1	AlgE1-AlgE7					1245:1255	AlgE1-AlgE7	1245:1255	AlgE1-AlgE7	1245:1255	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	5	4	theme	mechanical	1089:1098	arg1	strength					1100:1107	mechanical strength	1089:1107	mechanical strength	1089:1107	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	2	5	theme	G	436:436	arg1	residues					439:446	guluronic acid (G) residues	420:446	guluronic acid (G) residues of more than 100 residues	420:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	2	5	theme	G	436:436	arg1	residues					465:472	more than 100 residues	451:472	more than 100 residues	451:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	5	6	theme	stable	1034:1039	arg1	gels					1056:1059	stable, low syneretic gels	1034:1059	stable, low syneretic gels	1034:1059	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	5	7	from	alginates	954:962	arg1	present					943:949	present	943:949	present	943:949	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	1	8	theme	microbial	158:166	arg1	alginates					168:176	microbial alginates	158:176	microbial alginates as a source for strong gelling material	158:216	With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications.					
30844259	1	8	theme	microbial	158:166	arg1	alternative					238:248	an alternative	235:248	an alternative for advanced applications	235:274	With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications.					
30844259	8	9	theme	comparable	1587:1596	arg1	rigidity					1608:1615	comparable or higher rigidity	1587:1615	comparable or higher rigidity	1587:1615	Furthermore, poly-M epimerized with AlgE1 in vitro form gels with comparable or higher rigidity and gel strength than gels made from brown seaweed alginate with matching G-content.					
30844259	6	10	theme	different	1198:1206	arg1	epimerases					1233:1242	seven different extracellular mannuronan epimerases	1192:1242	seven different extracellular mannuronan epimerases (AlgE1-AlgE7)	1192:1256	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	6	10	theme	different	1198:1206	arg1	AlgE1-AlgE7					1245:1255	AlgE1-AlgE7	1245:1255	AlgE1-AlgE7	1245:1255	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	2	11	theme	residues	465:472	arg1	residues					439:446	guluronic acid (G) residues	420:446	guluronic acid (G) residues of more than 100 residues	420:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	2	11	theme	residues	465:472	arg1	residues					465:472	more than 100 residues	451:472	more than 100 residues	451:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	0	12	from	Biosynthesis	0:11	arg1	Alginate					59:66	Alginate	59:66	Alginate	59:66	Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii.					
30844259	5	13	theme	VLG	919:921	arg1	structures					923:932	these VLG structures	913:932	these VLG structures	913:932	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	3	14	with	acid	570:573	arg1	G-blocks					597:604	shorter G-blocks	589:604	shorter G-blocks	589:604	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	1	15	theme	advanced	254:261	arg1	applications					263:274	advanced applications	254:274	advanced applications	254:274	With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications.					
30844259	5	16	from	vinelandii	981:990	arg1	alginates					954:962	alginates	954:962	alginates from Azotobacter vinelandii	954:990	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	2	17	contain	contain	338:344	arg2	fraction					348:355	a fraction	346:355	a fraction of very long G-blocks (VLG)	346:383	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	2	17	contain	contain	338:344	arg2	sequences					407:415	consecutive sequences	395:415	consecutive sequences of guluronic acid (G) residues of more than 100 residues	395:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	2	17	contain	contain	338:344	arg1	alginate					306:313	alginate	306:313	alginate from algal sources	306:332	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	4	18	theme	gels	802:805	arg1	contraction					783:793	the contraction	779:793	the contraction of the gels	779:805	Hypothesized to function as reinforcement bars, VLG prevents the contraction of the gels during formation (syneresis) and increases the Young's modulus (strength of the gel).					
30844259	2	19	dep	contain	338:344	arg1	is					391:392	is	391:392	is	391:392	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	8	20	theme	higher	1601:1606	arg1	rigidity					1608:1615	comparable or higher rigidity	1587:1615	comparable or higher rigidity	1587:1615	Furthermore, poly-M epimerized with AlgE1 in vitro form gels with comparable or higher rigidity and gel strength than gels made from brown seaweed alginate with matching G-content.					
30844259	5	21	theme	algal	1112:1116	arg1	alginates					1118:1126	algal alginates	1112:1126	algal alginates with the similar monomeric composition	1112:1165	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	4	22	dep	increases	840:848	arg1	strength					871:878	strength	871:878	strength of the gel	871:889	Hypothesized to function as reinforcement bars, VLG prevents the contraction of the gels during formation (syneresis) and increases the Young's modulus (strength of the gel).					
30844259	5	23	from	comparable	1075:1084	arg1	strength					1100:1107	mechanical strength	1089:1107	mechanical strength	1089:1107	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	2	24	from	sources	326:332	arg1	alginate					306:313	alginate	306:313	alginate from algal sources	306:332	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	8	25	theme	gel	1621:1623	arg1	strength					1625:1632	gel strength	1621:1632	gel strength	1621:1632	Furthermore, poly-M epimerized with AlgE1 in vitro form gels with comparable or higher rigidity and gel strength than gels made from brown seaweed alginate with matching G-content.					
30844259	3	26	dep	in	535:536	arg1	vitro					538:542	vitro	538:542	vitro	538:542	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	9	27	theme	commercial	1745:1754	arg1	production					1765:1774	commercial alginate production	1745:1774	commercial alginate production from microbial sources	1745:1797	These findings strengthen the viability of commercial alginate production from microbial sources.					
30844259	2	28	theme	G-blocks	370:377	arg1	fraction					348:355	a fraction	346:355	a fraction of very long G-blocks (VLG)	346:383	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	4	29	theme	gel	887:889	arg1	strength					871:878	strength	871:878	strength of the gel	871:889	Hypothesized to function as reinforcement bars, VLG prevents the contraction of the gels during formation (syneresis) and increases the Young's modulus (strength of the gel).					
30844259	3	30	contain	have	672:675	arg1	VLG					668:670	VLG	668:670	VLG	668:670	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	3	30	contain	have	672:675	arg2	influence					685:693	a large influence	677:693	a large influence on gelling properties	677:715	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	2	31	theme	long	365:368	arg1	G-blocks					370:377	very long G-blocks	360:377	very long G-blocks (VLG)	360:383	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	2	31	theme	long	365:368	arg1	VLG					380:382	VLG	380:382	VLG	380:382	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	2	32	theme	consecutive	395:405	arg1	sequences					407:415	consecutive sequences	395:415	consecutive sequences of guluronic acid (G) residues of more than 100 residues	395:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	5	33	dep	stable	1034:1039	arg1	low					1042:1044	low	1042:1044	low	1042:1044	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	6	34	theme	long	1339:1342	arg1	G-blocks					1344:1351	the long G-blocks	1335:1351	the long G-blocks	1335:1351	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	3	35	theme	large	679:683	arg1	influence					685:693	a large influence	677:693	a large influence on gelling properties	677:715	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	3	36	theme	gelling	492:498	arg1	properties					500:509	the gelling properties	488:509	the gelling properties of these materials	488:528	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	4	37	theme	reinforcement	746:758	arg1	bars					760:763	reinforcement bars	746:763	reinforcement bars	746:763	Hypothesized to function as reinforcement bars, VLG prevents the contraction of the gels during formation (syneresis) and increases the Young's modulus (strength of the gel).					
30844259	7	38	theme	catalytic	1459:1467	arg1	sites					1469:1473	two catalytic sites	1455:1473	two catalytic sites	1455:1473	The data implies evidence for a processive mode of action and the necessity of two catalytic sites to obtain the observed epimerization pattern.					
30844259	9	39	from	sources	1791:1797	arg1	viability					1732:1740	the viability	1728:1740	the viability of commercial alginate production from microbial sources	1728:1797	These findings strengthen the viability of commercial alginate production from microbial sources.					
30844259	9	39	from	sources	1791:1797	arg1	production					1765:1774	commercial alginate production	1745:1774	commercial alginate production from microbial sources	1745:1797	These findings strengthen the viability of commercial alginate production from microbial sources.					
30844259	1	40	theme	strong	194:199	arg1	material					209:216	strong gelling material	194:216	strong gelling material	194:216	With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications.					
30844259	0	41	theme	Guluronic	34:42	arg1	Acid-Blocks					44:54	Long Guluronic Acid-Blocks	29:54	Long Guluronic Acid-Blocks	29:54	Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii.					
30844259	3	42	theme	shorter	589:595	arg1	G-blocks					597:604	shorter G-blocks	589:604	shorter G-blocks	589:604	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	3	43	theme	in	535:536	arg1	poly-M					576:581	poly-M	576:581	poly-M	576:581	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	3	43	theme	in	535:536	arg1	acid					570:573	epimerized polymannuronic acid	544:573	in vitro epimerized polymannuronic acid (poly-M)	535:582	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	1	44	theme	gelling	201:207	arg1	material					209:216	strong gelling material	194:216	strong gelling material	194:216	With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications.					
30844259	2	45	theme	acid	430:433	arg1	residues					439:446	guluronic acid (G) residues	420:446	guluronic acid (G) residues of more than 100 residues	420:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	2	45	theme	acid	430:433	arg1	residues					465:472	more than 100 residues	451:472	more than 100 residues	451:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	3	46	theme	gelling	698:704	arg1	properties					706:715	gelling properties	698:715	gelling properties	698:715	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	0	47	theme	Long	29:32	arg1	Acid-Blocks					44:54	Long Guluronic Acid-Blocks	29:54	Long Guluronic Acid-Blocks	29:54	Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii.					
30844259	5	48	theme	Azotobacter	969:979	arg1	vinelandii					981:990	Azotobacter vinelandii	969:990	Azotobacter vinelandii	969:990	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	9	49	from	viability	1732:1740	arg1	sources					1791:1797	microbial sources	1781:1797	microbial sources	1781:1797	These findings strengthen the viability of commercial alginate production from microbial sources.					
30844259	5	50	attach	present	943:949	arg2	structures					923:932	these VLG structures	913:932	these VLG structures	913:932	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	5	50	attach	present	943:949	arg1	alginates					954:962	alginates	954:962	alginates from Azotobacter vinelandii	954:990	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	2	51	theme	guluronic	420:428	arg1	residues					439:446	guluronic acid (G) residues	420:446	guluronic acid (G) residues of more than 100 residues	420:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	2	51	theme	guluronic	420:428	arg1	residues					465:472	more than 100 residues	451:472	more than 100 residues	451:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	5	52	theme	syneretic	1046:1054	arg1	gels					1056:1059	stable, low syneretic gels	1034:1059	stable, low syneretic gels	1034:1059	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	6	53	theme	bifunctional	1277:1288	arg1	AlgE1					1300:1304	only the bifunctional epimerase AlgE1	1268:1304	only the bifunctional epimerase AlgE1	1268:1304	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	6	53	theme	bifunctional	1277:1288	arg1	able					1318:1321	able	1318:1321	able	1318:1321	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	7	54	theme	action	1427:1432	arg1	necessity					1442:1450	the necessity	1438:1450	the necessity of two catalytic sites	1438:1473	The data implies evidence for a processive mode of action and the necessity of two catalytic sites to obtain the observed epimerization pattern.					
30844259	7	54	theme	action	1427:1432	arg1	mode					1419:1422	a processive mode	1406:1422	a processive mode of action	1406:1432	The data implies evidence for a processive mode of action and the necessity of two catalytic sites to obtain the observed epimerization pattern.					
30844259	3	55	theme	materials	520:528	arg1	properties					500:509	the gelling properties	488:509	the gelling properties of these materials	488:528	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	8	56	with	gels	1577:1580	arg1	rigidity					1608:1615	comparable or higher rigidity	1587:1615	comparable or higher rigidity	1587:1615	Furthermore, poly-M epimerized with AlgE1 in vitro form gels with comparable or higher rigidity and gel strength than gels made from brown seaweed alginate with matching G-content.					
30844259	8	56	with	gels	1577:1580	arg1	strength					1625:1632	gel strength	1621:1632	gel strength	1621:1632	Furthermore, poly-M epimerized with AlgE1 in vitro form gels with comparable or higher rigidity and gel strength than gels made from brown seaweed alginate with matching G-content.					
30844259	1	57	theme	present	113:119	arg1	accessibility					121:133	the present accessibility	109:133	the present accessibility of algal raw material	109:155	With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications.					
30844259	0	58	theme	Acid-Blocks	44:54	arg1	Function					17:24	Function	17:24	Function	17:24	Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii.					
30844259	0	58	theme	Acid-Blocks	44:54	arg1	Biosynthesis					0:11	Biosynthesis	0:11	Biosynthesis	0:11	Biosynthesis and Function of Long Guluronic Acid-Blocks in Alginate Produced by Azotobacter vinelandii.					
30844259	7	59	theme	observed	1489:1496	arg1	pattern					1512:1518	the observed epimerization pattern	1485:1518	the observed epimerization pattern	1485:1518	The data implies evidence for a processive mode of action and the necessity of two catalytic sites to obtain the observed epimerization pattern.					
30844259	7	60	theme	processive	1408:1417	arg1	mode					1419:1422	a processive mode	1406:1422	a processive mode of action	1406:1432	The data implies evidence for a processive mode of action and the necessity of two catalytic sites to obtain the observed epimerization pattern.					
30844259	8	61	theme	seaweed	1660:1666	arg1	alginate					1668:1675	brown seaweed alginate	1654:1675	brown seaweed alginate	1654:1675	Furthermore, poly-M epimerized with AlgE1 in vitro form gels with comparable or higher rigidity and gel strength than gels made from brown seaweed alginate with matching G-content.					
30844259	9	62	theme	alginate	1756:1763	arg1	production					1765:1774	commercial alginate production	1745:1774	commercial alginate production from microbial sources	1745:1797	These findings strengthen the viability of commercial alginate production from microbial sources.					
30844259	7	63	theme	epimerization	1498:1510	arg1	pattern					1512:1518	the observed epimerization pattern	1485:1518	the observed epimerization pattern	1485:1518	The data implies evidence for a processive mode of action and the necessity of two catalytic sites to obtain the observed epimerization pattern.					
30844259	6	64	theme	mannuronan	1222:1231	arg1	epimerases					1233:1242	seven different extracellular mannuronan epimerases	1192:1242	seven different extracellular mannuronan epimerases (AlgE1-AlgE7)	1192:1256	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	6	64	theme	mannuronan	1222:1231	arg1	AlgE1-AlgE7					1245:1255	AlgE1-AlgE7	1245:1255	AlgE1-AlgE7	1245:1255	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	8	65	theme	brown	1654:1658	arg1	alginate					1668:1675	brown seaweed alginate	1654:1675	brown seaweed alginate	1654:1675	Furthermore, poly-M epimerized with AlgE1 in vitro form gels with comparable or higher rigidity and gel strength than gels made from brown seaweed alginate with matching G-content.					
30844259	2	66	theme	algal	320:324	arg1	sources					326:332	algal sources	320:332	algal sources	320:332	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	9	67	theme	production	1765:1774	arg1	viability					1732:1740	the viability	1728:1740	the viability of commercial alginate production from microbial sources	1728:1797	These findings strengthen the viability of commercial alginate production from microbial sources.					
30844259	5	68	from	strength	1100:1107	arg1	comparable					1075:1084	comparable	1075:1084	comparable	1075:1084	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	6	69	theme	epimerase	1290:1298	arg1	AlgE1					1300:1304	only the bifunctional epimerase AlgE1	1268:1304	only the bifunctional epimerase AlgE1	1268:1304	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	6	69	theme	epimerase	1290:1298	arg1	able					1318:1321	able	1318:1321	able	1318:1321	The bacterium expresses seven different extracellular mannuronan epimerases (AlgE1-AlgE7), of which only the bifunctional epimerase AlgE1 seems to be able to generate the long G-blocks when acting on poly-M.					
30844259	1	70	theme	algal	138:142	arg1	material					148:155	algal raw material	138:155	algal raw material	138:155	With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications.					
30844259	5	71	from	present	943:949	arg1	alginates					954:962	alginates	954:962	alginates from Azotobacter vinelandii	954:990	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	5	72	located	present	943:949	arg2	structures					923:932	these VLG structures	913:932	these VLG structures	913:932	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	5	72	located	present	943:949	arg1	alginates					954:962	alginates	954:962	alginates from Azotobacter vinelandii	954:990	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	3	73	theme	epimerized	544:553	arg1	poly-M					576:581	poly-M	576:581	poly-M	576:581	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	3	73	theme	epimerized	544:553	arg1	acid					570:573	epimerized polymannuronic acid	544:573	in vitro epimerized polymannuronic acid (poly-M)	535:582	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	1	74	theme	raw	144:146	arg1	material					148:155	algal raw material	138:155	algal raw material	138:155	With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications.					
30844259	9	75	theme	microbial	1781:1789	arg1	sources					1791:1797	microbial sources	1781:1797	microbial sources	1781:1797	These findings strengthen the viability of commercial alginate production from microbial sources.					
30844259	7	76	theme	sites	1469:1473	arg1	necessity					1442:1450	the necessity	1438:1450	the necessity of two catalytic sites	1438:1473	The data implies evidence for a processive mode of action and the necessity of two catalytic sites to obtain the observed epimerization pattern.					
30844259	7	76	theme	sites	1469:1473	arg1	mode					1419:1422	a processive mode	1406:1422	a processive mode of action	1406:1432	The data implies evidence for a processive mode of action and the necessity of two catalytic sites to obtain the observed epimerization pattern.					
30844259	2	77	theme	residues	439:446	arg1	sequences					407:415	consecutive sequences	395:415	consecutive sequences of guluronic acid (G) residues of more than 100 residues	395:472	Recently, we have shown that alginate from algal sources all contain a fraction of very long G-blocks (VLG), that is, consecutive sequences of guluronic acid (G) residues of more than 100 residues.					
30844259	3	78	theme	polymannuronic	555:568	arg1	poly-M					576:581	poly-M	576:581	poly-M	576:581	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	3	78	theme	polymannuronic	555:568	arg1	acid					570:573	epimerized polymannuronic acid	544:573	in vitro epimerized polymannuronic acid (poly-M)	535:582	By comparing the gelling properties of these materials with in vitro epimerized polymannuronic acid (poly-M) with shorter G-blocks, but comparable with the G-content, we could demonstrate that VLG have a large influence on gelling properties.					
30844259	5	79	with	alginates	1118:1126	arg1	composition					1155:1165	the similar monomeric composition	1133:1165	the similar monomeric composition	1133:1165	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	1	80	theme	material	148:155	arg1	accessibility					121:133	the present accessibility	109:133	the present accessibility of algal raw material	109:155	With the present accessibility of algal raw material, microbial alginates as a source for strong gelling material are evaluated as an alternative for advanced applications.					
30844259	5	81	theme	monomeric	1145:1153	arg1	composition					1155:1165	the similar monomeric composition	1133:1165	the similar monomeric composition	1133:1165	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30844259	5	82	theme	similar	1137:1143	arg1	composition					1155:1165	the similar monomeric composition	1133:1165	the similar monomeric composition	1133:1165	Here we report that these VLG structures are also present in alginates from Azotobacter vinelandii and that these polymers consequently form stable, low syneretic gels with calcium, comparable in mechanical strength to algal alginates with the similar monomeric composition.					
30063585	0	0	theme	Milk	74:77	arg1	Products					79:86	Shelf-stable Human Milk Products	55:86	Shelf-stable Human Milk Products	55:86	Nutritional Comparison of Raw, Holder Pasteurized, and Shelf-stable Human Milk Products.					
30063585	11	1	theme	long-term	1604:1612	arg1	choice					1622:1627	a long-term feeding choice	1602:1627	a long-term feeding choice	1602:1627	Thiamine losses are clinically significant, and fortification may be necessary if SS donor milk is a long-term feeding choice.					
30063585	11	1	theme	long-term	1604:1612	arg1	milk					1594:1597	SS donor milk	1585:1597	SS donor milk	1585:1597	Thiamine losses are clinically significant, and fortification may be necessary if SS donor milk is a long-term feeding choice.					
30063585	5	2	theme	SS	664:665	arg1	samples					667:673	raw, HP, and SS samples	651:673	samples	667:673	RESULTS Percent fat, percent solids, and lactose were similar between raw, HP, and SS samples.					
30063585	9	3	theme	fat	1358:1360	arg1	levels					1390:1395	similar fat, lactose, and total protein levels	1350:1395	similar fat, lactose, and total protein levels	1350:1395	CONCLUSIONS Macronutrient content is relatively unaffected by processing; Holder pasteurization and retort processing maintain similar fat, lactose, and total protein levels.					
30063585	0	4	theme	Human	68:72	arg1	Products					79:86	Shelf-stable Human Milk Products	55:86	Shelf-stable Human Milk Products	55:86	Nutritional Comparison of Raw, Holder Pasteurized, and Shelf-stable Human Milk Products.					
30063585	1	5	from	pool	272:275	arg1	milk					253:256	raw human milk	243:256	raw human milk from the same pool	243:275	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	1	5	from	pool	272:275	arg1	HP					235:236	HP	235:236	HP	235:236	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	1	5	from	pool	272:275	arg1	pasteurized					222:232	Holder pasteurized	215:232	Holder pasteurized (HP)	215:237	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	1	6	theme	shelf-stable	147:158	arg1	SS					161:162	SS	161:162	SS	161:162	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	1	6	theme	shelf-stable	147:158	arg1	milk					171:174	shelf-stable (SS) human milk	147:174	shelf-stable (SS) human milk	147:174	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	6	7	theme	protein	797:803	arg1	differences					805:815	protein differences	797:815	protein differences	797:815	Total protein was statistically increased in SS samples when compared to raw (P = 0.005) and HP (P < 0.001) samples, but protein differences were not clinically relevant (raw = 15.1 mg/mL, HP = 14.8 mg/mL, and SS = 15.8 mg/mL).					
30063585	5	8	theme	Percent	589:595	arg1	fat					597:599	Percent fat	589:599	Percent fat	589:599	RESULTS Percent fat, percent solids, and lactose were similar between raw, HP, and SS samples.					
30063585	5	8	theme	Percent	589:595	arg1	RESULTS					581:587	RESULTS Percent fat, percent solids, and lactose	581:628	RESULTS Percent fat, percent solids, and lactose	581:628	RESULTS Percent fat, percent solids, and lactose were similar between raw, HP, and SS samples.					
30063585	7	9	theme	only	919:922	arg1	acid					930:933	the only amino acid	915:933	the only amino acid impacted by processing	915:956	Lysine was the only amino acid impacted by processing, and its destruction increased as heat increased (raw = 0.85 mg/100 mL, HP = 0.77 mg/100 mL, SS = 0.68 mg/100 mL).					
30063585	7	9	theme	only	919:922	arg1	Lysine					904:909	Lysine	904:909	Lysine	904:909	Lysine was the only amino acid impacted by processing, and its destruction increased as heat increased (raw = 0.85 mg/100 mL, HP = 0.77 mg/100 mL, SS = 0.68 mg/100 mL).					
30063585	1	10	dep	assess	109:114	arg1	aim					102:104	aim	102:104	aim	102:104	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	9	11	theme	CONCLUSIONS	1223:1233	arg1	content					1249:1255	CONCLUSIONS Macronutrient content	1223:1255	CONCLUSIONS Macronutrient content	1223:1255	CONCLUSIONS Macronutrient content is relatively unaffected by processing; Holder pasteurization and retort processing maintain similar fat, lactose, and total protein levels.					
30063585	7	12	theme	amino	924:928	arg1	acid					930:933	the only amino acid	915:933	the only amino acid impacted by processing	915:956	Lysine was the only amino acid impacted by processing, and its destruction increased as heat increased (raw = 0.85 mg/100 mL, HP = 0.77 mg/100 mL, SS = 0.68 mg/100 mL).					
30063585	7	12	theme	amino	924:928	arg1	Lysine					904:909	Lysine	904:909	Lysine	904:909	Lysine was the only amino acid impacted by processing, and its destruction increased as heat increased (raw = 0.85 mg/100 mL, HP = 0.77 mg/100 mL, SS = 0.68 mg/100 mL).					
30063585	9	13	theme	Macronutrient	1235:1247	arg1	content					1249:1255	CONCLUSIONS Macronutrient content	1223:1255	CONCLUSIONS Macronutrient content	1223:1255	CONCLUSIONS Macronutrient content is relatively unaffected by processing; Holder pasteurization and retort processing maintain similar fat, lactose, and total protein levels.					
30063585	11	14	theme	Thiamine	1503:1510	arg1	losses					1512:1517	Thiamine losses	1503:1517	Thiamine losses	1503:1517	Thiamine losses are clinically significant, and fortification may be necessary if SS donor milk is a long-term feeding choice.					
30063585	5	15	theme	percent	602:608	arg1	solids					610:615	percent solids	602:615	percent solids	602:615	RESULTS Percent fat, percent solids, and lactose were similar between raw, HP, and SS samples.					
30063585	6	16	theme	SS	721:722	arg1	samples					724:730	SS samples	721:730	SS samples	721:730	Total protein was statistically increased in SS samples when compared to raw (P = 0.005) and HP (P < 0.001) samples, but protein differences were not clinically relevant (raw = 15.1 mg/mL, HP = 14.8 mg/mL, and SS = 15.8 mg/mL).					
30063585	8	17	theme	raw	1171:1173	arg1	0.24 mg/L					1184:1192	0.24 mg/L	1184:1192	0.24 mg/L	1184:1192	Total thiamine was significantly decreased in SS samples (0.14 mg/L; P < 0.01) when compared with raw samples (0.24 mg/L) and HP samples (0.26 mg/L).					
30063585	8	17	theme	raw	1171:1173	arg1	samples					1175:1181	raw samples	1171:1181	raw samples (0.24 mg/L)	1171:1193	Total thiamine was significantly decreased in SS samples (0.14 mg/L; P < 0.01) when compared with raw samples (0.24 mg/L) and HP samples (0.26 mg/L).					
30063585	4	18	theme	total	530:534	arg1	protein					536:542	total protein	530:542	total protein	530:542	Samples were analyzed for percent fat, percent solids, total protein, lactose, amino acids, and thiamine.					
30063585	11	19	theme	feeding	1614:1620	arg1	choice					1622:1627	a long-term feeding choice	1602:1627	a long-term feeding choice	1602:1627	Thiamine losses are clinically significant, and fortification may be necessary if SS donor milk is a long-term feeding choice.					
30063585	11	19	theme	feeding	1614:1620	arg1	milk					1594:1597	SS donor milk	1585:1597	SS donor milk	1585:1597	Thiamine losses are clinically significant, and fortification may be necessary if SS donor milk is a long-term feeding choice.					
30063585	9	20	theme	protein	1382:1388	arg1	levels					1390:1395	similar fat, lactose, and total protein levels	1350:1395	similar fat, lactose, and total protein levels	1350:1395	CONCLUSIONS Macronutrient content is relatively unaffected by processing; Holder pasteurization and retort processing maintain similar fat, lactose, and total protein levels.					
30063585	0	21	theme	Nutritional	0:10	arg1	Comparison					12:21	Nutritional Comparison	0:21	Nutritional Comparison of Raw	0:28	Nutritional Comparison of Raw, Holder Pasteurized, and Shelf-stable Human Milk Products.					
30063585	3	22	theme	SS	463:464	arg1	product					466:472	an SS product	460:472	an SS product	460:472	From this pool, 36 samples were taken; 12 samples were kept raw, 12 samples were HP, and 12 samples were retort processed to create an SS product.					
30063585	1	23	theme	human	165:169	arg1	SS					161:162	SS	161:162	SS	161:162	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	1	23	theme	human	165:169	arg1	milk					171:174	shelf-stable (SS) human milk	147:174	shelf-stable (SS) human milk	147:174	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	0	24	theme	Raw	26:28	arg1	Comparison					12:21	Nutritional Comparison	0:21	Nutritional Comparison of Raw	0:28	Nutritional Comparison of Raw, Holder Pasteurized, and Shelf-stable Human Milk Products.					
30063585	0	24	theme	Raw	26:28	arg1	Products					79:86	Shelf-stable Human Milk Products	55:86	Shelf-stable Human Milk Products	55:86	Nutritional Comparison of Raw, Holder Pasteurized, and Shelf-stable Human Milk Products.					
30063585	1	25	theme	raw	243:245	arg1	milk					253:256	raw human milk	243:256	raw human milk from the same pool	243:275	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	2	26	from	mothers	307:313	arg1	samples					291:297	METHODS Milk samples	278:297	METHODS Milk samples from 60 mothers	278:313	METHODS Milk samples from 60 mothers were pooled.					
30063585	1	27	theme	milk	171:174	arg1	composition					132:142	the nutritional composition	116:142	the nutritional composition of shelf-stable (SS) human milk	116:174	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	11	28	theme	SS	1585:1586	arg1	choice					1622:1627	a long-term feeding choice	1602:1627	a long-term feeding choice	1602:1627	Thiamine losses are clinically significant, and fortification may be necessary if SS donor milk is a long-term feeding choice.					
30063585	11	28	theme	SS	1585:1586	arg1	milk					1594:1597	SS donor milk	1585:1597	SS donor milk	1585:1597	Thiamine losses are clinically significant, and fortification may be necessary if SS donor milk is a long-term feeding choice.					
30063585	1	29	theme	human	247:251	arg1	milk					253:256	raw human milk	243:256	raw human milk from the same pool	243:275	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	11	30	theme	donor	1588:1592	arg1	choice					1622:1627	a long-term feeding choice	1602:1627	a long-term feeding choice	1602:1627	Thiamine losses are clinically significant, and fortification may be necessary if SS donor milk is a long-term feeding choice.					
30063585	11	30	theme	donor	1588:1592	arg1	milk					1594:1597	SS donor milk	1585:1597	SS donor milk	1585:1597	Thiamine losses are clinically significant, and fortification may be necessary if SS donor milk is a long-term feeding choice.					
30063585	1	31	theme	nutritional	120:130	arg1	composition					132:142	the nutritional composition	116:142	the nutritional composition of shelf-stable (SS) human milk	116:174	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	8	32	theme	SS	1119:1120	arg1	samples					1122:1128	SS samples	1119:1128	SS samples (0.14 mg/L; P < 0.01)	1119:1150	Total thiamine was significantly decreased in SS samples (0.14 mg/L; P < 0.01) when compared with raw samples (0.24 mg/L) and HP samples (0.26 mg/L).					
30063585	8	33	theme	Total	1073:1077	arg1	thiamine					1079:1086	Total thiamine	1073:1086	Total thiamine	1073:1086	Total thiamine was significantly decreased in SS samples (0.14 mg/L; P < 0.01) when compared with raw samples (0.24 mg/L) and HP samples (0.26 mg/L).					
30063585	4	34	theme	amino	554:558	arg1	acids					560:564	amino acids	554:564	amino acids	554:564	Samples were analyzed for percent fat, percent solids, total protein, lactose, amino acids, and thiamine.					
30063585	6	35	theme	HP	769:770	arg1	P < 0.001					773:781	P < 0.001	773:781	P < 0.001	773:781	Total protein was statistically increased in SS samples when compared to raw (P = 0.005) and HP (P < 0.001) samples, but protein differences were not clinically relevant (raw = 15.1 mg/mL, HP = 14.8 mg/mL, and SS = 15.8 mg/mL).					
30063585	6	35	theme	HP	769:770	arg1	samples					784:790	raw (P = 0.005) and HP (P < 0.001) samples	749:790	samples	784:790	Total protein was statistically increased in SS samples when compared to raw (P = 0.005) and HP (P < 0.001) samples, but protein differences were not clinically relevant (raw = 15.1 mg/mL, HP = 14.8 mg/mL, and SS = 15.8 mg/mL).					
30063585	10	36	theme	retort	1450:1455	arg1	processing					1457:1466	retort processing	1450:1466	retort processing	1450:1466	Lysine and thiamine were significantly decreased by retort processing, but not by Holder pasteurization.					
30063585	8	37	theme	HP	1199:1200	arg1	0.26 mg/L					1211:1219	0.26 mg/L	1211:1219	0.26 mg/L	1211:1219	Total thiamine was significantly decreased in SS samples (0.14 mg/L; P < 0.01) when compared with raw samples (0.24 mg/L) and HP samples (0.26 mg/L).					
30063585	8	37	theme	HP	1199:1200	arg1	samples					1202:1208	HP samples	1199:1208	HP samples (0.26 mg/L)	1199:1220	Total thiamine was significantly decreased in SS samples (0.14 mg/L; P < 0.01) when compared with raw samples (0.24 mg/L) and HP samples (0.26 mg/L).					
30063585	7	38	theme	increased	997:1005	arg1	heat					992:995	heat increased	992:1005	heat increased (raw = 0.85 mg/100 mL, HP = 0.77 mg/100 mL, SS = 0.68 mg/100 mL)	992:1070	Lysine was the only amino acid impacted by processing, and its destruction increased as heat increased (raw = 0.85 mg/100 mL, HP = 0.77 mg/100 mL, SS = 0.68 mg/100 mL).					
30063585	7	39	dep	heat	992:995	arg1	HP = 0.77 mg/100 mL					1030:1048	HP = 0.77 mg/100 mL	1030:1048	HP = 0.77 mg/100 mL	1030:1048	Lysine was the only amino acid impacted by processing, and its destruction increased as heat increased (raw = 0.85 mg/100 mL, HP = 0.77 mg/100 mL, SS = 0.68 mg/100 mL).					
30063585	7	39	dep	heat	992:995	arg1	raw = 0.85 mg/100 mL					1008:1027	raw = 0.85 mg/100 mL	1008:1027	raw = 0.85 mg/100 mL	1008:1027	Lysine was the only amino acid impacted by processing, and its destruction increased as heat increased (raw = 0.85 mg/100 mL, HP = 0.77 mg/100 mL, SS = 0.68 mg/100 mL).					
30063585	7	39	dep	heat	992:995	arg1	SS = 0.68 mg/100 mL					1051:1069	SS = 0.68 mg/100 mL	1051:1069	SS = 0.68 mg/100 mL	1051:1069	Lysine was the only amino acid impacted by processing, and its destruction increased as heat increased (raw = 0.85 mg/100 mL, HP = 0.77 mg/100 mL, SS = 0.68 mg/100 mL).					
30063585	6	40	dep	relevant	837:844	arg1	HP = 14.8 mg/mL					865:879	HP = 14.8 mg/mL	865:879	HP = 14.8 mg/mL	865:879	Total protein was statistically increased in SS samples when compared to raw (P = 0.005) and HP (P < 0.001) samples, but protein differences were not clinically relevant (raw = 15.1 mg/mL, HP = 14.8 mg/mL, and SS = 15.8 mg/mL).					
30063585	6	40	dep	relevant	837:844	arg1	SS = 15.8 mg/mL					886:900	SS = 15.8 mg/mL	886:900	SS = 15.8 mg/mL	886:900	Total protein was statistically increased in SS samples when compared to raw (P = 0.005) and HP (P < 0.001) samples, but protein differences were not clinically relevant (raw = 15.1 mg/mL, HP = 14.8 mg/mL, and SS = 15.8 mg/mL).					
30063585	6	40	dep	relevant	837:844	arg1	raw = 15.1 mg/mL					847:862	raw = 15.1 mg/mL	847:862	raw = 15.1 mg/mL	847:862	Total protein was statistically increased in SS samples when compared to raw (P = 0.005) and HP (P < 0.001) samples, but protein differences were not clinically relevant (raw = 15.1 mg/mL, HP = 14.8 mg/mL, and SS = 15.8 mg/mL).					
30063585	5	41	theme	raw	651:653	arg1	HP					656:657	raw, HP, and SS samples	651:673	HP	656:657	RESULTS Percent fat, percent solids, and lactose were similar between raw, HP, and SS samples.					
30063585	1	42	theme	same	267:270	arg1	pool					272:275	the same pool	263:275	the same pool	263:275	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	6	43	theme	Total	676:680	arg1	protein					682:688	Total protein	676:688	Total protein	676:688	Total protein was statistically increased in SS samples when compared to raw (P = 0.005) and HP (P < 0.001) samples, but protein differences were not clinically relevant (raw = 15.1 mg/mL, HP = 14.8 mg/mL, and SS = 15.8 mg/mL).					
30063585	2	44	theme	Milk	286:289	arg1	samples					291:297	METHODS Milk samples	278:297	METHODS Milk samples from 60 mothers	278:313	METHODS Milk samples from 60 mothers were pooled.					
30063585	8	45	dep	0.14 mg/L	1131:1139	arg1	P < 0.01					1142:1149	P < 0.01	1142:1149	0.14 mg/L; P < 0.01	1131:1149	Total thiamine was significantly decreased in SS samples (0.14 mg/L; P < 0.01) when compared with raw samples (0.24 mg/L) and HP samples (0.26 mg/L).					
30063585	2	46	theme	METHODS	278:284	arg1	samples					291:297	METHODS Milk samples	278:297	METHODS Milk samples from 60 mothers	278:313	METHODS Milk samples from 60 mothers were pooled.					
30063585	1	47	theme	nutritional	192:202	arg1	profile					204:210	the nutritional profile	188:210	the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool	188:275	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	9	48	theme	total	1376:1380	arg1	protein					1382:1388	total protein	1376:1388	total protein	1376:1388	CONCLUSIONS Macronutrient content is relatively unaffected by processing; Holder pasteurization and retort processing maintain similar fat, lactose, and total protein levels.					
30063585	9	49	theme	lactose	1363:1369	arg1	levels					1390:1395	similar fat, lactose, and total protein levels	1350:1395	similar fat, lactose, and total protein levels	1350:1395	CONCLUSIONS Macronutrient content is relatively unaffected by processing; Holder pasteurization and retort processing maintain similar fat, lactose, and total protein levels.					
30063585	10	50	theme	Holder	1480:1485	arg1	pasteurization					1487:1500	Holder pasteurization	1480:1500	Holder pasteurization	1480:1500	Lysine and thiamine were significantly decreased by retort processing, but not by Holder pasteurization.					
30063585	9	51	theme	retort	1323:1328	arg1	processing					1330:1339	retort processing	1323:1339	retort processing	1323:1339	CONCLUSIONS Macronutrient content is relatively unaffected by processing; Holder pasteurization and retort processing maintain similar fat, lactose, and total protein levels.					
30063585	0	52	theme	Shelf-stable	55:66	arg1	Products					79:86	Shelf-stable Human Milk Products	55:86	Shelf-stable Human Milk Products	55:86	Nutritional Comparison of Raw, Holder Pasteurized, and Shelf-stable Human Milk Products.					
30063585	9	53	theme	Holder	1297:1302	arg1	pasteurization					1304:1317	Holder pasteurization	1297:1317	Holder pasteurization	1297:1317	CONCLUSIONS Macronutrient content is relatively unaffected by processing; Holder pasteurization and retort processing maintain similar fat, lactose, and total protein levels.					
30063585	8	54	dep	samples	1122:1128	arg1	0.14 mg/L					1131:1139	0.14 mg/L	1131:1139	0.14 mg/L; P < 0.01	1131:1149	Total thiamine was significantly decreased in SS samples (0.14 mg/L; P < 0.01) when compared with raw samples (0.24 mg/L) and HP samples (0.26 mg/L).					
30063585	4	55	theme	percent	514:520	arg1	solids					522:527	percent solids	514:527	percent solids	514:527	Samples were analyzed for percent fat, percent solids, total protein, lactose, amino acids, and thiamine.					
30063585	1	56	dep	OBJECTIVE	89:97	arg1	We					99:100	We	99:100	We	99:100	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	5	57	dep	RESULTS	581:587	arg1	RESULTS					581:587	RESULTS Percent fat, percent solids, and lactose	581:628	RESULTS Percent fat, percent solids, and lactose	581:628	RESULTS Percent fat, percent solids, and lactose were similar between raw, HP, and SS samples.					
30063585	5	57	dep	RESULTS	581:587	arg1	fat					597:599	Percent fat	589:599	Percent fat	589:599	RESULTS Percent fat, percent solids, and lactose were similar between raw, HP, and SS samples.					
30063585	5	57	dep	RESULTS	581:587	arg1	solids					610:615	percent solids	602:615	percent solids	602:615	RESULTS Percent fat, percent solids, and lactose were similar between raw, HP, and SS samples.					
30063585	5	57	dep	RESULTS	581:587	arg1	lactose					622:628	lactose	622:628	lactose	622:628	RESULTS Percent fat, percent solids, and lactose were similar between raw, HP, and SS samples.					
30063585	1	58	theme	Holder	215:220	arg1	HP					235:236	HP	235:236	HP	235:236	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	1	58	theme	Holder	215:220	arg1	pasteurized					222:232	Holder pasteurized	215:232	Holder pasteurized (HP)	215:237	OBJECTIVE We aim to assess the nutritional composition of shelf-stable (SS) human milk and compare the nutritional profile to Holder pasteurized (HP) and raw human milk from the same pool.					
30063585	9	59	theme	similar	1350:1356	arg1	levels					1390:1395	similar fat, lactose, and total protein levels	1350:1395	similar fat, lactose, and total protein levels	1350:1395	CONCLUSIONS Macronutrient content is relatively unaffected by processing; Holder pasteurization and retort processing maintain similar fat, lactose, and total protein levels.					
30063585	4	60	theme	percent	501:507	arg1	fat					509:511	percent fat	501:511	percent fat	501:511	Samples were analyzed for percent fat, percent solids, total protein, lactose, amino acids, and thiamine.					
29989826	4	0	theme	mass	840:843	arg1	proteomics					864:873	quantitative mass spectrometry-based proteomics	827:873	quantitative mass spectrometry-based proteomics	827:873	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	2	1	dep	two-dimensional	311:325	arg1	2D					328:329	2D	328:329	2D	328:329	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	1	2	theme	biological	158:167	arg1	research					169:176	cell biological research	153:176	cell biological research	153:176	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	5	3	theme	extra-	898:903	arg1	response					933:940	the extra- and intracellular proteomic response	894:940	the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers	894:992	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	4	4	theme	quantitative	827:838	arg1	proteomics					864:873	quantitative mass spectrometry-based proteomics	827:873	quantitative mass spectrometry-based proteomics	827:873	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	4	5	theme	culture	687:693	arg1	system					695:700	a 3D cell culture system	677:700	a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses	677:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	5	6	theme	proteomic	923:931	arg1	response					933:940	the extra- and intracellular proteomic response	894:940	the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers	894:992	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	7	7	theme	type	1187:1190	arg1	differences					1192:1202	cell type differences	1182:1202	cell type differences observed in 2D	1182:1217	Critically, cell type differences observed in 2D are often not preserved in 3D, which commonly closer resemble phenotypes observed in vivo.					
29989826	4	8	theme	isotope	767:773	arg1	labeling					775:782	stable isotope labeling	760:782	stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses	760:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	8	9	theme	extracellular	1322:1334	arg1	matrix					1336:1341	extracellular matrix	1322:1341	extracellular matrix	1322:1341	Especially, extracellular matrix and plasma membrane proteins are differentially regulated in 2D versus 3D.					
29989826	6	10	theme	culture	1150:1156	arg1	conditions					1158:1167	the culture conditions	1146:1167	the culture conditions	1146:1167	Both, control cells and CAF, change their proteomic composition based on the culture conditions.					
29989826	2	11	theme	two-dimensional	311:325	arg1	cultures					337:344	two-dimensional (2D) cell cultures	311:344	two-dimensional (2D) cell cultures	311:344	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	7	12	located	observed	1204:1211	arg2	differences					1192:1202	cell type differences	1182:1202	cell type differences observed in 2D	1182:1217	Critically, cell type differences observed in 2D are often not preserved in 3D, which commonly closer resemble phenotypes observed in vivo.					
29989826	7	12	located	observed	1204:1211	arg1	2D					1216:1217	2D	1216:1217	2D	1216:1217	Critically, cell type differences observed in 2D are often not preserved in 3D, which commonly closer resemble phenotypes observed in vivo.					
29989826	2	13	dep	in	382:383	arg1	vivo					385:388	vivo	385:388	vivo	385:388	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	4	14	theme	matrigel	739:746	arg1	mixture					713:719	a mixture	711:719	a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses	711:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	1	15	dep	tool	144:147	arg1	characterize					213:224	characterize	213:224	to characterize processes critical in human pathophysiology	210:268	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	1	15	dep	tool	144:147	arg1	study					181:185	study	181:185	to study molecular pathways	178:204	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	5	16	theme	intracellular	909:921	arg1	response					933:940	the extra- and intracellular proteomic response	894:940	the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers	894:992	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	4	17	theme	collagen	724:731	arg1	mixture					713:719	a mixture	711:719	a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses	711:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	4	18	theme	stable	760:765	arg1	labeling					775:782	stable isotope labeling	760:782	stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses	760:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	7	19	theme	cell	1182:1185	arg1	differences					1192:1202	cell type differences	1182:1202	cell type differences observed in 2D	1182:1217	Critically, cell type differences observed in 2D are often not preserved in 3D, which commonly closer resemble phenotypes observed in vivo.					
29989826	2	20	dep	reliability	446:456	arg1	the					442:444	the	442:444	the	442:444	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	1	21	theme	In	96:97	arg1	tool					144:147	an invaluable tool	130:147	an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology	130:268	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	1	21	theme	In	96:97	arg1	systems					118:124	In vitro cell culture systems	96:124	In vitro cell culture systems	96:124	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	6	22	theme	control	1079:1085	arg1	Both					1073:1076	Both	1073:1076	Both	1073:1076	Both, control cells and CAF, change their proteomic composition based on the culture conditions.					
29989826	6	22	theme	control	1079:1085	arg1	cells					1087:1091	control cells	1079:1091	control cells	1079:1091	Both, control cells and CAF, change their proteomic composition based on the culture conditions.					
29989826	0	23	theme	Cell	18:21	arg1	Conditions					31:40	Three-Dimensional Cell Culture Conditions	0:40	Three-Dimensional Cell Culture Conditions	0:40	Three-Dimensional Cell Culture Conditions Affect the Proteome of Cancer-Associated Fibroblasts.					
29989826	2	24	theme	cell	332:335	arg1	cultures					337:344	two-dimensional (2D) cell cultures	311:344	two-dimensional (2D) cell cultures	311:344	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	1	25	theme	molecular	187:195	arg1	pathways					197:204	molecular pathways	187:204	molecular pathways	187:204	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	0	26	theme	Three-Dimensional	0:16	arg1	Conditions					31:40	Three-Dimensional Cell Culture Conditions	0:40	Three-Dimensional Cell Culture Conditions	0:40	Three-Dimensional Cell Culture Conditions Affect the Proteome of Cancer-Associated Fibroblasts.					
29989826	5	27	attach	isolated	961:968	arg1	volunteers					983:992	healthy volunteers	975:992	healthy volunteers	975:992	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	5	27	attach	isolated	961:968	arg2	response					933:940	the extra- and intracellular proteomic response	894:940	the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers	894:992	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	1	28	theme	cell	105:108	arg1	tool					144:147	an invaluable tool	130:147	an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology	130:268	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	1	28	theme	cell	105:108	arg1	systems					118:124	In vitro cell culture systems	96:124	In vitro cell culture systems	96:124	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	4	29	theme	amenable	748:755	arg1	mixture					713:719	a mixture	711:719	a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses	711:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	5	30	theme	healthy	975:981	arg1	volunteers					983:992	healthy volunteers	975:992	healthy volunteers	975:992	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	1	31	theme	culture	110:116	arg1	tool					144:147	an invaluable tool	130:147	an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology	130:268	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	1	31	theme	culture	110:116	arg1	systems					118:124	In vitro cell culture systems	96:124	In vitro cell culture systems	96:124	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	4	32	dep	culture	807:813	arg1	analyses					875:882	analyses	875:882	analyses	875:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	0	33	theme	Culture	23:29	arg1	Conditions					31:40	Three-Dimensional Cell Culture Conditions	0:40	Three-Dimensional Cell Culture Conditions	0:40	Three-Dimensional Cell Culture Conditions Affect the Proteome of Cancer-Associated Fibroblasts.					
29989826	5	34	theme	skin	945:948	arg1	fibroblast					950:959	skin fibroblast	945:959	skin fibroblast	945:959	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	4	35	theme	cell	682:685	arg1	system					695:700	a 3D cell culture system	677:700	a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses	677:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	3	36	theme	cell	525:528	arg1	cultures					530:537	Three-dimensional (3D) cell cultures	502:537	Three-dimensional (3D) cell cultures	502:537	Three-dimensional (3D) cell cultures have been shown to closer mimic in vivo conditions and are commonly employed, for example, in pharmacological screens.					
29989826	5	37	theme	fibroblast	950:959	arg1	response					933:940	the extra- and intracellular proteomic response	894:940	the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers	894:992	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	5	38	theme	cancer-associated	1011:1027	arg1	fibroblasts					1029:1039	cancer-associated fibroblasts	1011:1039	cancer-associated fibroblasts (CAF)	1011:1045	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	5	38	theme	cancer-associated	1011:1027	arg1	CAF					1042:1044	CAF	1042:1044	CAF	1042:1044	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	5	39	dep	fibroblasts	1029:1039	arg1	comparison					997:1006	comparison	997:1006	comparison	997:1006	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	2	40	theme	results	493:499	arg1	conferrability					462:475	conferrability	462:475	conferrability	462:475	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	2	40	theme	results	493:499	arg1	reliability					446:456	reliability	446:456	reliability	446:456	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	3	41	theme	pharmacological	633:647	arg1	screens					649:655	pharmacological screens	633:655	pharmacological screens	633:655	Three-dimensional (3D) cell cultures have been shown to closer mimic in vivo conditions and are commonly employed, for example, in pharmacological screens.					
29989826	2	42	dep	raises	420:425	arg1	concerns					427:434	concerns	427:434	raises concerns about the reliability and conferrability of the obtained results	420:499	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	2	43	theme	obtained	484:491	arg1	results					493:499	the obtained results	480:499	the obtained results	480:499	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	8	44	theme	membrane	1354:1361	arg1	proteins					1363:1370	plasma membrane proteins	1347:1370	plasma membrane proteins	1347:1370	Especially, extracellular matrix and plasma membrane proteins are differentially regulated in 2D versus 3D.					
29989826	8	45	theme	plasma	1347:1352	arg1	proteins					1363:1370	plasma membrane proteins	1347:1370	plasma membrane proteins	1347:1370	Especially, extracellular matrix and plasma membrane proteins are differentially regulated in 2D versus 3D.					
29989826	6	46	theme	proteomic	1115:1123	arg1	composition					1125:1135	their proteomic composition	1109:1135	their proteomic composition	1109:1135	Both, control cells and CAF, change their proteomic composition based on the culture conditions.					
29989826	1	47	from	pathophysiology	254:268	arg1	critical					236:243	critical	236:243	critical	236:243	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	4	48	theme	cell	802:805	arg1	SILAC					816:820	SILAC	816:820	SILAC	816:820	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	4	48	theme	cell	802:805	arg1	culture					807:813	cell culture	802:813	cell culture (SILAC)	802:821	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	1	49	dep	In	96:97	arg1	vitro					99:103	vitro	99:103	vitro	99:103	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	2	50	from	conditions	297:306	arg1	cultures					337:344	two-dimensional (2D) cell cultures	311:344	two-dimensional (2D) cell cultures	311:344	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	1	51	from	critical	236:243	arg1	pathophysiology					254:268	human pathophysiology	248:268	human pathophysiology	248:268	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	4	52	from	acids	793:797	arg1	culture					807:813	cell culture	802:813	cell culture (SILAC)	802:821	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	4	52	from	acids	793:797	arg1	SILAC					816:820	SILAC	816:820	SILAC	816:820	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	4	52	from	acids	793:797	arg1	proteomics					864:873	quantitative mass spectrometry-based proteomics	827:873	quantitative mass spectrometry-based proteomics	827:873	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	1	53	theme	invaluable	133:142	arg1	tool					144:147	an invaluable tool	130:147	an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology	130:268	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	1	53	theme	invaluable	133:142	arg1	systems					118:124	In vitro cell culture systems	96:124	In vitro cell culture systems	96:124	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	3	54	theme	in	571:572	arg1	conditions					579:588	in vivo conditions	571:588	in vivo conditions	571:588	Three-dimensional (3D) cell cultures have been shown to closer mimic in vivo conditions and are commonly employed, for example, in pharmacological screens.					
29989826	1	55	theme	critical	236:243	arg1	processes					226:234	processes	226:234	processes critical in human pathophysiology	226:268	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	2	56	theme	experimental	284:295	arg1	conditions					297:306	the experimental conditions	280:306	the experimental conditions in two-dimensional (2D) cell cultures	280:344	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29989826	5	57	theme	3D	1050:1051	arg1	conditions					1061:1070	3D culture conditions	1050:1070	3D culture conditions	1050:1070	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	3	58	dep	Three-dimensional	502:518	arg1	3D					521:522	3D	521:522	3D	521:522	Three-dimensional (3D) cell cultures have been shown to closer mimic in vivo conditions and are commonly employed, for example, in pharmacological screens.					
29989826	4	59	theme	3D	679:680	arg1	system					695:700	a 3D cell culture system	677:700	a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses	677:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	4	60	theme	spectrometry-based	845:862	arg1	proteomics					864:873	quantitative mass spectrometry-based proteomics	827:873	quantitative mass spectrometry-based proteomics	827:873	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	3	61	theme	Three-dimensional	502:518	arg1	cultures					530:537	Three-dimensional (3D) cell cultures	502:537	Three-dimensional (3D) cell cultures	502:537	Three-dimensional (3D) cell cultures have been shown to closer mimic in vivo conditions and are commonly employed, for example, in pharmacological screens.					
29989826	0	62	theme	Fibroblasts	83:93	arg1	Proteome					53:60	the Proteome	49:60	the Proteome of Cancer-Associated Fibroblasts	49:93	Three-Dimensional Cell Culture Conditions Affect the Proteome of Cancer-Associated Fibroblasts.					
29989826	5	63	theme	culture	1053:1059	arg1	conditions					1061:1070	3D culture conditions	1050:1070	3D culture conditions	1050:1070	We study the extra- and intracellular proteomic response of skin fibroblast isolated from healthy volunteers in comparison to cancer-associated fibroblasts (CAF) on 3D culture conditions.					
29989826	4	64	theme	amino	787:791	arg1	acids					793:797	amino acids	787:797	amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses	787:882	Here, we introduce a 3D cell culture system based on a mixture of collagen I and matrigel amenable to stable isotope labeling by amino acids in cell culture (SILAC) and quantitative mass spectrometry-based proteomics analyses.					
29989826	3	65	dep	in	571:572	arg1	vivo					574:577	vivo	574:577	vivo	574:577	Three-dimensional (3D) cell cultures have been shown to closer mimic in vivo conditions and are commonly employed, for example, in pharmacological screens.					
29989826	1	66	theme	human	248:252	arg1	pathophysiology					254:268	human pathophysiology	248:268	human pathophysiology	248:268	In vitro cell culture systems are an invaluable tool for cell biological research to study molecular pathways and to characterize processes critical in human pathophysiology.					
29989826	0	67	theme	Cancer-Associated	65:81	arg1	Fibroblasts					83:93	Cancer-Associated Fibroblasts	65:93	Cancer-Associated Fibroblasts	65:93	Three-Dimensional Cell Culture Conditions Affect the Proteome of Cancer-Associated Fibroblasts.					
29989826	2	68	theme	in	382:383	arg1	situation					390:398	the in vivo situation	378:398	the in vivo situation	378:398	However, the experimental conditions in two-dimensional (2D) cell cultures often differ substantially from the in vivo situation, which continuously raises concerns about the reliability and conferrability of the obtained results.					
29761500	7	0	theme	expressed	1129:1137	arg1	genes					1139:1143	3577 differentially expressed genes	1109:1143	3577 differentially expressed genes	1109:1143	We performed Illumina high-throughput sequencing for SL and RL, and 3577 differentially expressed genes were identified.					
29761500	10	1	theme	higher	1576:1581	arg1	levels					1583:1588	higher levels	1576:1588	higher levels	1576:1588	Furthermore, transcription factors and other regulatory proteins previously associated with cuticle production were expressed at higher levels in SL than in RL.					
29761500	5	2	dep	dominated	817:825	arg1	whereas					876:882	whereas	876:882	whereas	876:882	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	9	3	theme	compositional	1391:1403	arg1	differences					1405:1415	compositional differences	1391:1415	compositional differences between these two leaf types	1391:1444	Many cuticle-associated genes exhibited differential expression levels that could be associated with compositional differences between these two leaf types.					
29761500	1	4	theme	plant	174:178	arg1	structure					201:209	a complex structure	191:209	a complex structure composed primarily of wax and cutin	191:245	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	1	4	theme	plant	174:178	arg1	cuticle					180:186	The plant cuticle	170:186	The plant cuticle	170:186	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	9	5	theme	leaf	1435:1438	arg1	types					1440:1444	these two leaf types	1425:1444	these two leaf types	1425:1444	Many cuticle-associated genes exhibited differential expression levels that could be associated with compositional differences between these two leaf types.					
29761500	10	6	theme	other	1486:1490	arg1	proteins					1503:1510	other regulatory proteins	1486:1510	other regulatory proteins	1486:1510	Furthermore, transcription factors and other regulatory proteins previously associated with cuticle production were expressed at higher levels in SL than in RL.					
29761500	3	7	theme	inflorescence	499:511	arg1	stem					513:516	the inflorescence stem	495:516	the inflorescence stem of the model extremophyte Thellungiella salsuginea	495:567	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	10	8	theme	transcription	1460:1472	arg1	factors					1474:1480	transcription factors	1460:1480	transcription factors	1460:1480	Furthermore, transcription factors and other regulatory proteins previously associated with cuticle production were expressed at higher levels in SL than in RL.					
29761500	3	9	theme	extremophyte	531:542	arg1	salsuginea					558:567	the model extremophyte Thellungiella salsuginea	521:567	the model extremophyte Thellungiella salsuginea	521:567	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	4	10	theme	lower	756:760	arg1	permeability					770:781	lower cuticle permeability	756:781	lower cuticle permeability	756:781	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
29761500	6	11	theme	cutin	960:964	arg1	monomers					966:973	The major cutin monomers	950:973	The major cutin monomers for both leaf types	950:993	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	6	11	theme	cutin	960:964	arg1	acids					1012:1016	C18:2 dioic acids	1000:1016	C18:2 dioic acids	1000:1016	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	0	12	theme	inflorescence	98:110	arg1	stem					112:115	inflorescence stem	98:115	inflorescence stem	98:115	Compositional and transcriptomic analysis associated with cuticle lipid production on rosette and inflorescence stem leaves in the extremophyte Thellungiella salsuginea.					
29761500	11	13	theme	stress	1871:1876	arg1	tolerance					1878:1886	improved stress tolerance	1862:1886	improved stress tolerance	1862:1886	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	3	14	theme	Thellungiella	544:556	arg1	salsuginea					558:567	the model extremophyte Thellungiella salsuginea	521:567	the model extremophyte Thellungiella salsuginea	521:567	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	5	15	theme	wax	908:910	arg1	acids					943:947	free very long-chain acids	922:947	free very long-chain acids	922:947	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	5	15	theme	wax	908:910	arg1	class					912:916	the most abundant wax class	890:916	the most abundant wax class	890:916	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	9	16	theme	Many	1290:1293	arg1	genes					1314:1318	Many cuticle-associated genes	1290:1318	Many cuticle-associated genes	1290:1318	Many cuticle-associated genes exhibited differential expression levels that could be associated with compositional differences between these two leaf types.					
29761500	7	17	theme	Illumina	1054:1061	arg1	sequencing					1079:1088	Illumina high-throughput sequencing	1054:1088	Illumina high-throughput sequencing for SL and RL	1054:1102	We performed Illumina high-throughput sequencing for SL and RL, and 3577 differentially expressed genes were identified.					
29761500	11	18	theme	leaf	1692:1695	arg1	cuticles					1697:1704	this extremophile's leaf cuticles	1672:1704	this extremophile's leaf cuticles	1672:1704	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	5	19	theme	C31	862:864	arg1	alkanes					830:836	alkanes	830:836	alkanes	830:836	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	5	19	theme	C31	862:864	arg1	homologs					866:873	the C29 and C31 homologs	850:873	homologs	866:873	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	5	20	theme	C29	854:856	arg1	alkanes					830:836	alkanes	830:836	alkanes	830:836	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	5	20	theme	C29	854:856	arg1	homologs					866:873	the C29 and C31 homologs	850:873	homologs	866:873	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	6	21	theme	major	954:958	arg1	monomers					966:973	The major cutin monomers	950:973	The major cutin monomers for both leaf types	950:993	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	6	21	theme	major	954:958	arg1	acids					1012:1016	C18:2 dioic acids	1000:1016	C18:2 dioic acids	1000:1016	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	4	22	contain	had	719:721	arg1	SL					715:716	SL	715:716	SL	715:716	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
29761500	4	22	contain	had	719:721	arg2	lipids					745:750	more abundant cuticle lipids	723:750	more abundant cuticle lipids	723:750	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
29761500	4	22	contain	had	719:721	arg2	permeability					770:781	lower cuticle permeability	756:781	lower cuticle permeability	756:781	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
29761500	4	22	contain	had	719:721	arg1	stem					702:705	stem leaves	702:712	stem leaves (SL)	702:717	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
29761500	7	23	theme	high-throughput	1063:1077	arg1	sequencing					1079:1088	Illumina high-throughput sequencing	1054:1088	Illumina high-throughput sequencing for SL and RL	1054:1102	We performed Illumina high-throughput sequencing for SL and RL, and 3577 differentially expressed genes were identified.					
29761500	3	24	theme	model	525:529	arg1	salsuginea					558:567	the model extremophyte Thellungiella salsuginea	521:567	the model extremophyte Thellungiella salsuginea	521:567	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	8	25	theme	lipid	1210:1214	arg1	biosynthesis					1216:1227	cuticular lipid biosynthesis	1200:1227	cuticular lipid biosynthesis	1200:1227	Sixty-five genes possibly involved in cuticular lipid biosynthesis, transport, or regulation was selected for further analysis.					
29761500	11	26	from	trait	1748:1752	arg1	environments					1765:1776	extreme environments	1757:1776	extreme environments	1757:1776	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	11	27	theme	cuticles	1697:1704	arg1	associations					1612:1623	The associations	1608:1623	The associations between gene expression	1608:1647	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	11	27	theme	cuticles	1697:1704	arg1	characteristics					1653:1667	characteristics	1653:1667	characteristics of this extremophile's leaf cuticles	1653:1704	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	11	28	theme	improved	1862:1869	arg1	tolerance					1878:1886	improved stress tolerance	1862:1886	improved stress tolerance	1862:1886	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	5	29	theme	free	922:925	arg1	acids					943:947	free very long-chain acids	922:947	free very long-chain acids	922:947	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	5	29	theme	free	922:925	arg1	class					912:916	the most abundant wax class	890:916	the most abundant wax class	890:916	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	11	30	theme	new	1712:1714	arg1	light					1716:1720	new light	1712:1720	new light	1712:1720	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	11	30	theme	new	1712:1714	arg1	trait					1748:1752	an adaptive trait	1736:1752	an adaptive trait in extreme environments	1736:1776	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	1	31	theme	complex	193:199	arg1	structure					201:209	a complex structure	191:209	a complex structure composed primarily of wax and cutin	191:245	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	1	31	theme	complex	193:199	arg1	cuticle					180:186	The plant cuticle	170:186	The plant cuticle	170:186	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	9	32	theme	differential	1330:1341	arg1	levels					1354:1359	differential expression levels	1330:1359	differential expression levels that could be associated with compositional differences between these two leaf types	1330:1444	Many cuticle-associated genes exhibited differential expression levels that could be associated with compositional differences between these two leaf types.					
29761500	11	33	theme	adaptive	1739:1746	arg1	light					1716:1720	new light	1712:1720	new light	1712:1720	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	11	33	theme	adaptive	1739:1746	arg1	trait					1748:1752	an adaptive trait	1736:1752	an adaptive trait in extreme environments	1736:1776	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	10	34	theme	regulatory	1492:1501	arg1	proteins					1503:1510	other regulatory proteins	1486:1510	other regulatory proteins	1486:1510	Furthermore, transcription factors and other regulatory proteins previously associated with cuticle production were expressed at higher levels in SL than in RL.					
29761500	1	35	contain	contains	257:264	arg2	glycerolipids					273:285	glycerolipids	273:285	glycerolipids	273:285	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	1	35	contain	contains	257:264	arg1	structure					201:209	a complex structure	191:209	a complex structure composed primarily of wax and cutin	191:245	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	1	35	contain	contains	257:264	arg2	phenolics					288:296	phenolics	288:296	phenolics	288:296	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	1	35	contain	contains	257:264	arg2	cutan					266:270	cutan	266:270	cutan	266:270	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	1	35	contain	contains	257:264	arg2	polysaccharides					299:313	polysaccharides	299:313	polysaccharides	299:313	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	1	35	contain	contains	257:264	arg1	cuticle					180:186	The plant cuticle	170:186	The plant cuticle	170:186	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	1	35	contain	contains	257:264	arg2	proteins					319:326	proteins	319:326	proteins	319:326	The plant cuticle is a complex structure composed primarily of wax and cutin, but also contains cutan, glycerolipids, phenolics, polysaccharides and proteins.					
29761500	0	36	theme	extremophyte	131:142	arg1	salsuginea					158:167	the extremophyte Thellungiella salsuginea	127:167	the extremophyte Thellungiella salsuginea	127:167	Compositional and transcriptomic analysis associated with cuticle lipid production on rosette and inflorescence stem leaves in the extremophyte Thellungiella salsuginea.					
29761500	3	37	dep	4-week-old	440:449	arg1	produced					458:465	produced	458:465	produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea	458:567	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	3	37	dep	4-week-old	440:449	arg1	leaves					451:456	leaves	451:456	leaves	451:456	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	3	38	theme	salsuginea	558:567	arg1	stem					513:516	the inflorescence stem	495:516	the inflorescence stem of the model extremophyte Thellungiella salsuginea	495:567	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	0	39	theme	transcriptomic	18:31	arg1	analysis					33:40	transcriptomic analysis	18:40	transcriptomic analysis	18:40	Compositional and transcriptomic analysis associated with cuticle lipid production on rosette and inflorescence stem leaves in the extremophyte Thellungiella salsuginea.					
29761500	9	40	theme	expression	1343:1352	arg1	levels					1354:1359	differential expression levels	1330:1359	differential expression levels that could be associated with compositional differences between these two leaf types	1330:1444	Many cuticle-associated genes exhibited differential expression levels that could be associated with compositional differences between these two leaf types.					
29761500	2	41	theme	important	350:358	arg1	role					371:374	an important protective role	347:374	an important protective role	347:374	The cuticle plays an important protective role as barrier between plants and their environment.					
29761500	8	42	theme	Sixty-five	1162:1171	arg1	genes					1173:1177	Sixty-five genes	1162:1177	Sixty-five genes possibly involved in cuticular lipid biosynthesis, transport, or regulation	1162:1253	Sixty-five genes possibly involved in cuticular lipid biosynthesis, transport, or regulation was selected for further analysis.					
29761500	6	43	theme	dioic	1006:1010	arg1	acids					1012:1016	C18:2 dioic acids	1000:1016	C18:2 dioic acids	1000:1016	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	6	43	theme	dioic	1006:1010	arg1	monomers					966:973	The major cutin monomers	950:973	The major cutin monomers for both leaf types	950:993	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	6	44	theme	C18:2	1028:1032	arg1	acids					1034:1038	18-OH C18:2 acids	1022:1038	18-OH C18:2 acids	1022:1038	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	0	45	theme	Thellungiella	144:156	arg1	salsuginea					158:167	the extremophyte Thellungiella salsuginea	127:167	the extremophyte Thellungiella salsuginea	127:167	Compositional and transcriptomic analysis associated with cuticle lipid production on rosette and inflorescence stem leaves in the extremophyte Thellungiella salsuginea.					
29761500	3	46	theme	chemical	651:658	arg1	analysis					672:679	chemical composition analysis	651:679	chemical composition analysis	651:679	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	11	47	theme	extreme	1757:1763	arg1	environments					1765:1776	extreme environments	1757:1776	extreme environments	1757:1776	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	6	48	theme	C18:2	1000:1004	arg1	acids					1012:1016	C18:2 dioic acids	1000:1016	C18:2 dioic acids	1000:1016	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	6	48	theme	C18:2	1000:1004	arg1	monomers					966:973	The major cutin monomers	950:973	The major cutin monomers for both leaf types	950:993	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	0	49	theme	lipid	66:70	arg1	production					72:81	cuticle lipid production	58:81	cuticle lipid production on rosette and inflorescence stem	58:115	Compositional and transcriptomic analysis associated with cuticle lipid production on rosette and inflorescence stem leaves in the extremophyte Thellungiella salsuginea.					
29761500	0	50	from	production	72:81	arg1	rosette					86:92	rosette	86:92	rosette	86:92	Compositional and transcriptomic analysis associated with cuticle lipid production on rosette and inflorescence stem leaves in the extremophyte Thellungiella salsuginea.					
29761500	0	50	from	production	72:81	arg1	stem					112:115	inflorescence stem	98:115	inflorescence stem	98:115	Compositional and transcriptomic analysis associated with cuticle lipid production on rosette and inflorescence stem leaves in the extremophyte Thellungiella salsuginea.					
29761500	3	51	theme	scanning	589:596	arg1	microscopy					607:616	scanning electron microscopy	589:616	scanning electron microscopy	589:616	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	9	52	theme	cuticle-associated	1295:1312	arg1	genes					1314:1318	Many cuticle-associated genes	1290:1318	Many cuticle-associated genes	1290:1318	Many cuticle-associated genes exhibited differential expression levels that could be associated with compositional differences between these two leaf types.					
29761500	0	53	theme	cuticle	58:64	arg1	production					72:81	cuticle lipid production	58:81	cuticle lipid production on rosette and inflorescence stem	58:115	Compositional and transcriptomic analysis associated with cuticle lipid production on rosette and inflorescence stem leaves in the extremophyte Thellungiella salsuginea.					
29761500	11	54	theme	new	1795:1797	arg1	information					1799:1809	new information	1795:1809	new information that may guide efforts to modify crop cuticles for improved stress tolerance	1795:1886	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	3	55	theme	composition	660:670	arg1	analysis					672:679	chemical composition analysis	651:679	chemical composition analysis	651:679	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	3	56	theme	electron	598:605	arg1	microscopy					607:616	scanning electron microscopy	589:616	scanning electron microscopy	589:616	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	11	57	theme	crop	1844:1847	arg1	cuticles					1849:1856	crop cuticles	1844:1856	crop cuticles for improved stress tolerance	1844:1886	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	10	58	from	SL	1593:1594	arg1	RL					1604:1605	RL	1604:1605	RL	1604:1605	Furthermore, transcription factors and other regulatory proteins previously associated with cuticle production were expressed at higher levels in SL than in RL.					
29761500	4	59	dep	stem	702:705	arg1	leaves					707:712	leaves	707:712	leaves	707:712	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
29761500	4	60	theme	cuticle	737:743	arg1	lipids					745:750	more abundant cuticle lipids	723:750	more abundant cuticle lipids	723:750	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
29761500	4	61	theme	cuticle	762:768	arg1	permeability					770:781	lower cuticle permeability	756:781	lower cuticle permeability	756:781	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
29761500	10	62	theme	cuticle	1539:1545	arg1	production					1547:1556	cuticle production	1539:1556	cuticle production	1539:1556	Furthermore, transcription factors and other regulatory proteins previously associated with cuticle production were expressed at higher levels in SL than in RL.					
29761500	5	63	theme	long-chain	932:941	arg1	acids					943:947	free very long-chain acids	922:947	free very long-chain acids	922:947	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	5	63	theme	long-chain	932:941	arg1	class					912:916	the most abundant wax class	890:916	the most abundant wax class	890:916	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	4	64	theme	abundant	728:735	arg1	lipids					745:750	more abundant cuticle lipids	723:750	more abundant cuticle lipids	723:750	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
29761500	5	65	theme	abundant	899:906	arg1	acids					943:947	free very long-chain acids	922:947	free very long-chain acids	922:947	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	5	65	theme	abundant	899:906	arg1	class					912:916	the most abundant wax class	890:916	the most abundant wax class	890:916	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	5	66	from	acids	943:947	arg1	RL					887:888	RL	887:888	RL	887:888	SL were dominated by alkanes, especially the C29 and C31 homologs, whereas in RL the most abundant wax class was free very long-chain acids.					
29761500	3	67	theme	cuticle	619:625	arg1	assays					640:645	cuticle permeability assays	619:645	cuticle permeability assays	619:645	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	2	68	theme	protective	360:369	arg1	role					371:374	an important protective role	347:374	an important protective role	347:374	The cuticle plays an important protective role as barrier between plants and their environment.					
29761500	8	69	theme	further	1272:1278	arg1	analysis					1280:1287	further analysis	1272:1287	further analysis	1272:1287	Sixty-five genes possibly involved in cuticular lipid biosynthesis, transport, or regulation was selected for further analysis.					
29761500	6	70	theme	leaf	984:987	arg1	types					989:993	both leaf types	979:993	both leaf types	979:993	The major cutin monomers for both leaf types were C18:2 dioic acids and 18-OH C18:2 acids.					
29761500	8	71	theme	cuticular	1200:1208	arg1	biosynthesis					1216:1227	cuticular lipid biosynthesis	1200:1227	cuticular lipid biosynthesis	1200:1227	Sixty-five genes possibly involved in cuticular lipid biosynthesis, transport, or regulation was selected for further analysis.					
29761500	3	72	theme	permeability	627:638	arg1	assays					640:645	cuticle permeability assays	619:645	cuticle permeability assays	619:645	In this paper, 4-week-old leaves produced either on the rosette or on the inflorescence stem of the model extremophyte Thellungiella salsuginea were examined using scanning electron microscopy, cuticle permeability assays and chemical composition analysis.					
29761500	11	73	theme	gene	1633:1636	arg1	expression					1638:1647	gene expression	1633:1647	gene expression	1633:1647	The associations between gene expression and characteristics of this extremophile's leaf cuticles sheds new light on cuticle as an adaptive trait in extreme environments, and contributes new information that may guide efforts to modify crop cuticles for improved stress tolerance.					
29761500	4	74	dep	rosette	788:794	arg1	leaves					796:801	leaves	796:801	leaves	796:801	Results showed that stem leaves (SL) had more abundant cuticle lipids and lower cuticle permeability than rosette leaves (RL).					
31761177	5	0	attach	presence	985:992	arg1	surface					1010:1016	the surface	1006:1016	the surface	1006:1016	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	0	attach	presence	985:992	arg2	AgNPs					997:1001	AgNPs	997:1001	AgNPs	997:1001	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	7	1	theme	distinct	1126:1133	arg1	zones					1146:1150	distinct inhibition zones	1126:1150	distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA)	1126:1497	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	6	2	theme	CoHy	1051:1054	arg1	samples					1056:1062	the CoHy samples	1047:1062	the CoHy samples	1047:1062	Antimicrobial properties of the CoHy samples were evaluated using agar diffusion tests.					
31761177	1	3	theme	silk	182:185	arg1	RSF					196:198	RSF	196:198	RSF	196:198	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	1	3	theme	silk	182:185	arg1	fibroin					187:193	regenerated silk fibroin	170:193	regenerated silk fibroin (RSF)	170:199	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	5	4	from	presence	985:992	arg1	surface					1010:1016	the surface	1006:1016	the surface	1006:1016	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	8	5	located	observed	1783:1790	arg2	proliferation					1765:1777	higher cell proliferation	1753:1777	higher cell proliferation	1753:1777	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	5	located	observed	1783:1790	arg1	CoHy					1799:1802	the CoHy	1795:1802	the CoHy without AgNPs	1795:1816	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	2	6	theme	cell	507:510	arg1	activities					512:521	cell activities	507:521	cell activities	507:521	Indeed, nano-silver has long been recognized as one of the most effective antimicrobial agents, and silk fibroin is well known for its capability of stimulating cell activities and facilitating tissue regeneration.					
31761177	7	7	theme	inhibition	1135:1144	arg1	zones					1146:1150	distinct inhibition zones	1126:1150	distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA)	1126:1497	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	8	8	theme	cell	1760:1763	arg1	proliferation					1765:1777	higher cell proliferation	1753:1777	higher cell proliferation	1753:1777	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	6	9	theme	diffusion	1090:1098	arg1	tests					1100:1104	agar diffusion tests	1085:1104	agar diffusion tests	1085:1104	Antimicrobial properties of the CoHy samples were evaluated using agar diffusion tests.					
31761177	7	10	dep	S.	1269:1270	arg1	aureus					1272:1277	S. aureus	1269:1277	S. aureus	1269:1277	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	5	11	theme	AgNPs	997:1001	arg1	presence					985:992	the presence	981:992	the presence of AgNPs on the surface	981:1016	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	8	12	theme	higher	1753:1758	arg1	proliferation					1765:1777	higher cell proliferation	1753:1777	higher cell proliferation	1753:1777	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	7	13	theme	Candida	1418:1424	arg1	C.					1436:1437	C.	1436:1437	C.	1436:1437	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	13	theme	Candida	1418:1424	arg1	albicans					1426:1433	Candida albicans	1418:1433	Candida albicans (C. albicans)	1418:1447	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	3	14	theme	synthesis	584:592	arg1	approach					594:601	a green synthesis approach	576:601	a green synthesis approach	576:601	In this study, a green synthesis approach was used to create a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs.					
31761177	1	15	theme	wound-related	294:306	arg1	infections					308:317	wound-related infections	294:317	wound-related infections	294:317	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	0	16	theme	biomedical	112:121	arg1	applications					123:134	biomedical applications	112:134	biomedical applications	112:134	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose nanocomposite hydrogels for biomedical applications.					
31761177	8	17	link	derived	1547:1553	arg1	cells					1572:1576	rat bone marrow derived mesenchymal stem cells	1531:1576	rat bone marrow derived mesenchymal stem cells (BMSCs)	1531:1584	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	17	link	derived	1547:1553	arg1	BMSCs					1579:1583	BMSCs	1579:1583	BMSCs	1579:1583	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	7	18	dep	P.	1402:1403	arg1	aeruginosa					1405:1414	P. aeruginosa	1402:1414	P. aeruginosa	1402:1414	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	8	19	theme	adhesion	1611:1618	arg1	similar					1673:1679	similar	1673:1679	similar	1673:1679	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	19	theme	adhesion	1611:1618	arg1	density					1620:1626	the adhesion density	1607:1626	the adhesion density of BMScs on the CoHy loaded with 1 mg/mL	1607:1667	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	20	theme	Cytocompatibility	1500:1516	arg1	studies					1518:1524	Cytocompatibility studies	1500:1524	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro	1500:1593	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	21	theme	regenerative	1834:1845	arg1	potentials					1847:1856	the regenerative potentials	1830:1856	the regenerative potentials of the RSF/CMC composite hydrogels	1830:1891	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	5	22	theme	electron	868:875	arg1	microscopy					877:886	Scanning electron microscopy	859:886	Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS)	859:940	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	22	theme	electron	868:875	arg1	XRD					966:968	XRD	966:968	XRD	966:968	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	22	theme	electron	868:875	arg1	SEM					889:891	SEM	889:891	SEM	889:891	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	2	23	theme	agents	434:439	arg1	one					394:396	one	394:396	one	394:396	Indeed, nano-silver has long been recognized as one of the most effective antimicrobial agents, and silk fibroin is well known for its capability of stimulating cell activities and facilitating tissue regeneration.					
31761177	2	23	theme	agents	434:439	arg1	agents					434:439	the most effective antimicrobial agents	401:439	the most effective antimicrobial agents	401:439	Indeed, nano-silver has long been recognized as one of the most effective antimicrobial agents, and silk fibroin is well known for its capability of stimulating cell activities and facilitating tissue regeneration.					
31761177	2	23	theme	agents	434:439	arg1	nano-silver					354:364	nano-silver	354:364	nano-silver	354:364	Indeed, nano-silver has long been recognized as one of the most effective antimicrobial agents, and silk fibroin is well known for its capability of stimulating cell activities and facilitating tissue regeneration.					
31761177	2	24	theme	tissue	540:545	arg1	regeneration					547:558	tissue regeneration	540:558	tissue regeneration	540:558	Indeed, nano-silver has long been recognized as one of the most effective antimicrobial agents, and silk fibroin is well known for its capability of stimulating cell activities and facilitating tissue regeneration.					
31761177	8	25	theme	rat	1531:1533	arg1	cells					1572:1576	rat bone marrow derived mesenchymal stem cells	1531:1576	rat bone marrow derived mesenchymal stem cells (BMSCs)	1531:1584	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	25	theme	rat	1531:1533	arg1	BMSCs					1579:1583	BMSCs	1579:1583	BMSCs	1579:1583	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	7	26	theme	major	1160:1164	arg1	microorganisms					1166:1179	major microorganisms	1160:1179	major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA)	1160:1497	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	5	27	theme	Material	829:836	arg1	characterizations					838:854	Material characterizations	829:854	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD)	829:969	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	2	28	theme	antimicrobial	420:432	arg1	agents					434:439	the most effective antimicrobial agents	401:439	the most effective antimicrobial agents	401:439	Indeed, nano-silver has long been recognized as one of the most effective antimicrobial agents, and silk fibroin is well known for its capability of stimulating cell activities and facilitating tissue regeneration.					
31761177	0	29	theme	green	15:19	arg1	synthesis					21:29	Photo-assisted green synthesis	0:29	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose	0:82	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose nanocomposite hydrogels for biomedical applications.					
31761177	5	30	theme	X-Ray	947:951	arg1	Diffraction					953:963	X-Ray Diffraction	947:963	X-Ray Diffraction	947:963	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	7	31	theme	wound	1190:1194	arg1	albicans					1426:1433	Candida albicans	1418:1433	Candida albicans (C. albicans)	1418:1447	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	31	theme	wound	1190:1194	arg1	aeruginosa					1390:1399	Pseudomonas aeruginosa	1378:1399	Pseudomonas aeruginosa (P. aeruginosa)	1378:1415	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	31	theme	wound	1190:1194	arg1	coli					1230:1233	Escherichia coli	1218:1233	Escherichia coli (E. coli)	1218:1243	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	31	theme	wound	1190:1194	arg1	albicans					1483:1490	Fluconazole-resistant Candida albicans	1453:1490	Fluconazole-resistant Candida albicans (FRCA)	1453:1497	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	31	theme	wound	1190:1194	arg1	infections					1196:1205	wound infections	1190:1205	wound infections	1190:1205	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	2	32	theme	effective	410:418	arg1	agents					434:439	the most effective antimicrobial agents	401:439	the most effective antimicrobial agents	401:439	Indeed, nano-silver has long been recognized as one of the most effective antimicrobial agents, and silk fibroin is well known for its capability of stimulating cell activities and facilitating tissue regeneration.					
31761177	0	33	theme	Photo-assisted	0:13	arg1	synthesis					21:29	Photo-assisted green synthesis	0:29	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose	0:82	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose nanocomposite hydrogels for biomedical applications.					
31761177	8	34	theme	marrow	1540:1545	arg1	cells					1572:1576	rat bone marrow derived mesenchymal stem cells	1531:1576	rat bone marrow derived mesenchymal stem cells (BMSCs)	1531:1584	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	34	theme	marrow	1540:1545	arg1	BMSCs					1579:1583	BMSCs	1579:1583	BMSCs	1579:1583	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	35	theme	control	1698:1704	arg1	group					1706:1710	the cell-only control group	1684:1710	the cell-only control group for the first 24 h of culture	1684:1740	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	5	36	theme	Energy	899:904	arg1	Spectroscopy					923:934	Energy Dispersive X-ray Spectroscopy	899:934	Energy Dispersive X-ray Spectroscopy (EDS)	899:940	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	36	theme	Energy	899:904	arg1	EDS					937:939	EDS	937:939	EDS	937:939	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	8	37	theme	bone	1535:1538	arg1	cells					1572:1576	rat bone marrow derived mesenchymal stem cells	1531:1576	rat bone marrow derived mesenchymal stem cells (BMSCs)	1531:1584	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	37	theme	bone	1535:1538	arg1	BMSCs					1579:1583	BMSCs	1579:1583	BMSCs	1579:1583	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	5	38	with	microscopy	877:886	arg1	Spectroscopy					923:934	Energy Dispersive X-ray Spectroscopy	899:934	Energy Dispersive X-ray Spectroscopy (EDS)	899:940	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	38	with	microscopy	877:886	arg1	EDS					937:939	EDS	937:939	EDS	937:939	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	7	39	located	found	1181:1185	arg1	coli					1230:1233	Escherichia coli	1218:1233	Escherichia coli (E. coli)	1218:1243	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	39	located	found	1181:1185	arg2	microorganisms					1166:1179	major microorganisms	1160:1179	major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA)	1160:1497	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	39	located	found	1181:1185	arg1	infections					1196:1205	wound infections	1190:1205	wound infections	1190:1205	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	39	located	found	1181:1185	arg1	albicans					1426:1433	Candida albicans	1418:1433	Candida albicans (C. albicans)	1418:1447	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	39	located	found	1181:1185	arg1	aeruginosa					1390:1399	Pseudomonas aeruginosa	1378:1399	Pseudomonas aeruginosa (P. aeruginosa)	1378:1415	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	39	located	found	1181:1185	arg1	albicans					1483:1490	Fluconazole-resistant Candida albicans	1453:1490	Fluconazole-resistant Candida albicans (FRCA)	1453:1497	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	3	40	theme	green	578:582	arg1	approach					594:601	a green synthesis approach	576:601	a green synthesis approach	576:601	In this study, a green synthesis approach was used to create a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs.					
31761177	8	41	theme	cell-only	1688:1696	arg1	group					1706:1710	the cell-only control group	1684:1710	the cell-only control group for the first 24 h of culture	1684:1740	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	5	42	theme	Scanning	859:866	arg1	microscopy					877:886	Scanning electron microscopy	859:886	Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS)	859:940	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	42	theme	Scanning	859:866	arg1	XRD					966:968	XRD	966:968	XRD	966:968	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	42	theme	Scanning	859:866	arg1	SEM					889:891	SEM	889:891	SEM	889:891	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	43	with	Diffraction	953:963	arg1	Spectroscopy					923:934	Energy Dispersive X-ray Spectroscopy	899:934	Energy Dispersive X-ray Spectroscopy (EDS)	899:940	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	43	with	Diffraction	953:963	arg1	EDS					937:939	EDS	937:939	EDS	937:939	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	1	44	theme	tissue	326:331	arg1	engineering					333:343	tissue engineering	326:343	tissue engineering	326:343	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	7	45	dep	E.	1236:1237	arg1	coli					1239:1242	E. coli	1236:1242	E. coli	1236:1242	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	3	46	theme	RSF	653:655	arg1	hydrogel					634:641	a composite hydrogel	622:641	a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs	622:728	In this study, a green synthesis approach was used to create a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs.					
31761177	3	46	theme	RSF	653:655	arg1	CoHy					644:647	CoHy	644:647	CoHy	644:647	In this study, a green synthesis approach was used to create a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs.					
31761177	1	47	theme	Silver	137:142	arg1	AgNPs					159:163	AgNPs	159:163	AgNPs	159:163	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	1	47	theme	Silver	137:142	arg1	nanoparticles					144:156	Silver nanoparticles	137:156	Silver nanoparticles (AgNPs)	137:164	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	8	48	theme	mesenchymal	1555:1565	arg1	cells					1572:1576	rat bone marrow derived mesenchymal stem cells	1531:1576	rat bone marrow derived mesenchymal stem cells (BMSCs)	1531:1584	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	48	theme	mesenchymal	1555:1565	arg1	BMSCs					1579:1583	BMSCs	1579:1583	BMSCs	1579:1583	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	2	49	theme	silk	446:449	arg1	fibroin					451:457	silk fibroin	446:457	silk fibroin	446:457	Indeed, nano-silver has long been recognized as one of the most effective antimicrobial agents, and silk fibroin is well known for its capability of stimulating cell activities and facilitating tissue regeneration.					
31761177	0	50	theme	doped	41:45	arg1	cellulose					74:82	silver doped silk fibroin/carboxymethyl cellulose	34:82	silver doped silk fibroin/carboxymethyl cellulose	34:82	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose nanocomposite hydrogels for biomedical applications.					
31761177	8	51	theme	derived	1547:1553	arg1	cells					1572:1576	rat bone marrow derived mesenchymal stem cells	1531:1576	rat bone marrow derived mesenchymal stem cells (BMSCs)	1531:1584	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	51	theme	derived	1547:1553	arg1	BMSCs					1579:1583	BMSCs	1579:1583	BMSCs	1579:1583	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	52	theme	hydrogels	1883:1891	arg1	potentials					1847:1856	the regenerative potentials	1830:1856	the regenerative potentials of the RSF/CMC composite hydrogels	1830:1891	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	53	with	studies	1518:1524	arg1	cells					1572:1576	rat bone marrow derived mesenchymal stem cells	1531:1576	rat bone marrow derived mesenchymal stem cells (BMSCs)	1531:1584	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	53	with	studies	1518:1524	arg1	BMSCs					1579:1583	BMSCs	1579:1583	BMSCs	1579:1583	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	1	54	theme	significant	225:235	arg1	interests					237:245	significant interests	225:245	significant interests for their potential applications in preventing wound-related infections and in tissue engineering	225:343	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	6	55	theme	agar	1085:1088	arg1	tests					1100:1104	agar diffusion tests	1085:1104	agar diffusion tests	1085:1104	Antimicrobial properties of the CoHy samples were evaluated using agar diffusion tests.					
31761177	8	56	theme	composite	1873:1881	arg1	hydrogels					1883:1891	the RSF/CMC composite hydrogels	1861:1891	the RSF/CMC composite hydrogels	1861:1891	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	3	57	dep	RSF	653:655	arg1	loaded					712:717	loaded	712:717	loaded with AgNPs	712:728	In this study, a green synthesis approach was used to create a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs.					
31761177	3	57	dep	RSF	653:655	arg1	stabilized					657:666	stabilized	657:666	stabilized with CarboxymethylCellulose-Na (CMC-Na)	657:706	In this study, a green synthesis approach was used to create a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs.					
31761177	8	58	theme	first	1720:1724	arg1	24 h					1726:1729	the first 24 h	1716:1729	the first 24 h of culture	1716:1740	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	59	theme	BMScs	1631:1635	arg1	similar					1673:1679	similar	1673:1679	similar	1673:1679	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	59	theme	BMScs	1631:1635	arg1	density					1620:1626	the adhesion density	1607:1626	the adhesion density of BMScs on the CoHy loaded with 1 mg/mL	1607:1667	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	0	60	theme	fibroin/carboxymethyl	52:72	arg1	cellulose					74:82	silver doped silk fibroin/carboxymethyl cellulose	34:82	silver doped silk fibroin/carboxymethyl cellulose	34:82	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose nanocomposite hydrogels for biomedical applications.					
31761177	7	61	dep	C.	1436:1437	arg1	albicans					1439:1446	C. albicans	1436:1446	C. albicans	1436:1446	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	8	62	from	density	1620:1626	arg1	CoHy					1644:1647	the CoHy	1640:1647	the CoHy loaded with 1 mg/mL	1640:1667	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	0	63	theme	silk	47:50	arg1	cellulose					74:82	silver doped silk fibroin/carboxymethyl cellulose	34:82	silver doped silk fibroin/carboxymethyl cellulose	34:82	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose nanocomposite hydrogels for biomedical applications.					
31761177	8	64	theme	RSF/CMC	1865:1871	arg1	hydrogels					1883:1891	the RSF/CMC composite hydrogels	1861:1891	the RSF/CMC composite hydrogels	1861:1891	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	3	65	used	used	607:610	arg2	approach					594:601	a green synthesis approach	576:601	a green synthesis approach	576:601	In this study, a green synthesis approach was used to create a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs.					
31761177	7	66	theme	Fluconazole-resistant	1453:1473	arg1	FRCA					1493:1496	FRCA	1493:1496	FRCA	1493:1496	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	66	theme	Fluconazole-resistant	1453:1473	arg1	albicans					1483:1490	Fluconazole-resistant Candida albicans	1453:1490	Fluconazole-resistant Candida albicans (FRCA)	1453:1497	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	67	dep	S.	1309:1310	arg1	epidermidis					1312:1322	S. epidermidis	1309:1322	S. epidermidis	1309:1322	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	8	68	theme	culture	1734:1740	arg1	24 h					1726:1729	the first 24 h	1716:1729	the first 24 h of culture	1716:1740	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	4	69	theme	swelling	737:744	arg1	ratios					746:751	Their swelling ratios	731:751	Their swelling ratios	731:751	Their swelling ratios were up to 59 g/g when tested in different physiologically relevant fluids.					
31761177	7	70	dep	coli	1230:1233	arg1	aureus					1363:1368	Staphylococcus aureus	1348:1368	Staphylococcus aureus	1348:1368	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	70	dep	coli	1230:1233	arg1	MRSA					1371:1374	MRSA	1371:1374	Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA)	1218:1375	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	4	71	theme	different	786:794	arg1	fluids					821:826	different physiologically relevant fluids	786:826	different physiologically relevant fluids	786:826	Their swelling ratios were up to 59 g/g when tested in different physiologically relevant fluids.					
31761177	5	72	theme	X-ray	917:921	arg1	Spectroscopy					923:934	Energy Dispersive X-ray Spectroscopy	899:934	Energy Dispersive X-ray Spectroscopy (EDS)	899:940	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	72	theme	X-ray	917:921	arg1	EDS					937:939	EDS	937:939	EDS	937:939	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	6	73	theme	Antimicrobial	1019:1031	arg1	properties					1033:1042	Antimicrobial properties	1019:1042	Antimicrobial properties of the CoHy samples	1019:1062	Antimicrobial properties of the CoHy samples were evaluated using agar diffusion tests.					
31761177	3	74	theme	composite	624:632	arg1	hydrogel					634:641	a composite hydrogel	622:641	a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs	622:728	In this study, a green synthesis approach was used to create a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs.					
31761177	3	74	theme	composite	624:632	arg1	CoHy					644:647	CoHy	644:647	CoHy	644:647	In this study, a green synthesis approach was used to create a composite hydrogel (CoHy) of RSF stabilized with CarboxymethylCellulose-Na (CMC-Na) and loaded with AgNPs.					
31761177	6	75	theme	samples	1056:1062	arg1	properties					1033:1042	Antimicrobial properties	1019:1042	Antimicrobial properties of the CoHy samples	1019:1062	Antimicrobial properties of the CoHy samples were evaluated using agar diffusion tests.					
31761177	5	76	theme	Dispersive	906:915	arg1	Spectroscopy					923:934	Energy Dispersive X-ray Spectroscopy	899:934	Energy Dispersive X-ray Spectroscopy (EDS)	899:940	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	5	76	theme	Dispersive	906:915	arg1	EDS					937:939	EDS	937:939	EDS	937:939	Material characterizations by Scanning electron microscopy (SEM) with Energy Dispersive X-ray Spectroscopy (EDS), and X-Ray Diffraction (XRD) confirmed the presence of AgNPs on the surface.					
31761177	1	77	theme	potential	257:265	arg1	applications					267:278	their potential applications	251:278	their potential applications in preventing wound-related infections and in tissue engineering	251:343	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	0	78	theme	cellulose	74:82	arg1	synthesis					21:29	Photo-assisted green synthesis	0:29	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose	0:82	Photo-assisted green synthesis of silver doped silk fibroin/carboxymethyl cellulose nanocomposite hydrogels for biomedical applications.					
31761177	7	79	theme	Candida	1475:1481	arg1	FRCA					1493:1496	FRCA	1493:1496	FRCA	1493:1496	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	7	79	theme	Candida	1475:1481	arg1	albicans					1483:1490	Fluconazole-resistant Candida albicans	1453:1490	Fluconazole-resistant Candida albicans (FRCA)	1453:1497	The results showed distinct inhibition zones against major microorganisms found in wound infections, including Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Staphylococcus epidermidis (S. epidermidis), Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (P. aeruginosa), Candida albicans (C. albicans) and Fluconazole-resistant Candida albicans (FRCA).					
31761177	4	80	theme	relevant	812:819	arg1	fluids					821:826	different physiologically relevant fluids	786:826	different physiologically relevant fluids	786:826	Their swelling ratios were up to 59 g/g when tested in different physiologically relevant fluids.					
31761177	1	81	theme	regenerated	170:180	arg1	RSF					196:198	RSF	196:198	RSF	196:198	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	1	81	theme	regenerated	170:180	arg1	fibroin					187:193	regenerated silk fibroin	170:193	regenerated silk fibroin (RSF)	170:199	Silver nanoparticles (AgNPs) and regenerated silk fibroin (RSF) have recently attracted significant interests for their potential applications in preventing wound-related infections and in tissue engineering.					
31761177	8	82	theme	stem	1567:1570	arg1	cells					1572:1576	rat bone marrow derived mesenchymal stem cells	1531:1576	rat bone marrow derived mesenchymal stem cells (BMSCs)	1531:1584	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
31761177	8	82	theme	stem	1567:1570	arg1	BMSCs					1579:1583	BMSCs	1579:1583	BMSCs	1579:1583	Cytocompatibility studies with rat bone marrow derived mesenchymal stem cells (BMSCs) in vitro showed that the adhesion density of BMScs on the CoHy loaded with 1 mg/mL was similar to the cell-only control group for the first 24 h of culture; moreover, higher cell proliferation was observed on the CoHy without AgNPs, indicating the regenerative potentials of the RSF/CMC composite hydrogels.					
29655710	5	0	theme	volatile	748:755	arg1	molecules					757:765	volatile molecules	748:765	volatile molecules	748:765	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	5	1	theme	coated	614:619	arg1	fruit					621:625	The coated fruit	610:625	The coated fruit	610:625	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	7	2	theme	thin	996:999	arg1	layer					1001:1005	a non-sticky and homogeneous thin layer	967:1005	a non-sticky and homogeneous thin layer	967:1005	Finally, morphological analysis performed on fruit fractured surface, highlighted the formation of a non-sticky and homogeneous thin layer.					
29655710	2	3	theme	antimicrobial	324:336	arg1	coatings					247:254	edible coatings	240:254	edible coatings based on pectin and honey	240:280	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	2	3	theme	antimicrobial	324:336	arg1	agents					338:343	dehydrating and antimicrobial agents	308:343	dehydrating and antimicrobial agents of cut fruit samples	308:364	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	5	4	theme	molecules	757:765	arg1	profile					767:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	7	5	theme	non-sticky	969:978	arg1	layer					1001:1005	a non-sticky and homogeneous thin layer	967:1005	a non-sticky and homogeneous thin layer	967:1005	Finally, morphological analysis performed on fruit fractured surface, highlighted the formation of a non-sticky and homogeneous thin layer.					
29655710	8	6	theme	fruit	1048:1052	arg1	able					1120:1123	able	1120:1123	able	1120:1123	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	8	6	theme	fruit	1048:1052	arg1	process					1066:1072	the novel fruit dehydration process	1038:1072	the novel fruit dehydration process	1038:1072	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	5	7	theme	polyphenol	679:688	arg1	contents					704:711	enriched polyphenol and vitamin C contents	670:711	enriched polyphenol and vitamin C contents	670:711	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	5	8	theme	contents	704:711	arg1	profile					767:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	8	9	theme	dehydration	1054:1064	arg1	able					1120:1123	able	1120:1123	able	1120:1123	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	8	9	theme	dehydration	1054:1064	arg1	process					1066:1072	the novel fruit dehydration process	1038:1072	the novel fruit dehydration process	1038:1072	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	7	10	theme	morphological	877:889	arg1	analysis					891:898	morphological analysis	877:898	morphological analysis	877:898	Finally, morphological analysis performed on fruit fractured surface, highlighted the formation of a non-sticky and homogeneous thin layer.					
29655710	5	11	theme	enhanced	637:644	arg1	profile					767:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	6	12	theme	antimicrobial	790:802	arg1	activity					804:811	the antimicrobial activity	786:811	the antimicrobial activity against Pseudomonas and Escherichia coli	786:852	Moreover, the antimicrobial activity against Pseudomonas and Escherichia coli was assessed.					
29655710	0	13	theme	properties	103:112	arg1	Enhancement					73:83	Enhancement	73:83	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.	0:136	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	8	14	theme	fruits	1179:1184	arg1	safety					1146:1151	safety	1146:1151	safety	1146:1151	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	8	14	theme	fruits	1179:1184	arg1	quality					1157:1163	quality	1157:1163	quality	1157:1163	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	1	15	theme	novel	155:159	arg1	process					177:183	a novel and sustainable process	153:183	a novel and sustainable process for the fruit dehydration	153:209	In this paper, a novel and sustainable process for the fruit dehydration was described.					
29655710	0	16	theme	functional	92:101	arg1	properties					103:112	the functional properties	88:112	the functional properties of coated dried fruits	88:135	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	8	17	dep	safety	1146:1151	arg1	the					1142:1144	the	1142:1144	the	1142:1144	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	2	18	theme	samples	358:364	arg1	coatings					247:254	edible coatings	240:254	edible coatings based on pectin and honey	240:280	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	2	18	theme	samples	358:364	arg1	agents					338:343	dehydrating and antimicrobial agents	308:343	dehydrating and antimicrobial agents of cut fruit samples	308:364	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	2	19	from	processes	444:452	arg1	preservation					399:410	the fruit preservation	389:410	the fruit preservation from irreversible deteriorative processes	389:452	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	2	20	used	used	300:303	arg2	coatings					247:254	edible coatings	240:254	edible coatings based on pectin and honey	240:280	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	2	20	used	used	300:303	arg2	agents					338:343	dehydrating and antimicrobial agents	308:343	dehydrating and antimicrobial agents of cut fruit samples	308:364	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	8	21	theme	pectin-honey	1094:1105	arg1	coating					1107:1113	pectin-honey coating	1094:1113	pectin-honey coating	1094:1113	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	2	22	theme	fruit	352:356	arg1	samples					358:364	cut fruit samples	348:364	cut fruit samples	348:364	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	5	23	theme	vitamin	694:700	arg1	C					702:702	vitamin C	694:702	vitamin C	694:702	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	2	24	theme	cut	348:350	arg1	samples					358:364	cut fruit samples	348:364	cut fruit samples	348:364	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	2	25	theme	fruit	393:397	arg1	preservation					399:410	the fruit preservation	389:410	the fruit preservation from irreversible deteriorative processes	389:452	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	8	26	theme	dehydrated	1168:1177	arg1	fruits					1179:1184	dehydrated fruits	1168:1184	dehydrated fruits	1168:1184	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	0	27	theme	coated	117:122	arg1	fruits					130:135	coated dried fruits	117:135	coated dried fruits	117:135	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	1	28	theme	sustainable	165:175	arg1	process					177:183	a novel and sustainable process	153:183	a novel and sustainable process for the fruit dehydration	153:209	In this paper, a novel and sustainable process for the fruit dehydration was described.					
29655710	4	29	theme	dehydrated	581:590	arg1	control					600:606	control	600:606	control	600:606	The analysis were performed also on uncoated dehydrated fruits (control).					
29655710	4	29	theme	dehydrated	581:590	arg1	fruits					592:597	uncoated dehydrated fruits	572:597	uncoated dehydrated fruits (control)	572:607	The analysis were performed also on uncoated dehydrated fruits (control).					
29655710	0	30	theme	bioactive	42:50	arg1	agent					52:56	novel dehydrating bioactive agent	24:56	novel dehydrating bioactive agent for cut fruit	24:70	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	0	31	theme	fruits	130:135	arg1	properties					103:112	the functional properties	88:112	the functional properties of coated dried fruits	88:135	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	5	32	theme	C	702:702	arg1	contents					704:711	enriched polyphenol and vitamin C contents	670:711	enriched polyphenol and vitamin C contents	670:711	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	0	33	theme	dehydrating	30:40	arg1	agent					52:56	novel dehydrating bioactive agent	24:56	novel dehydrating bioactive agent for cut fruit	24:70	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	0	34	theme	dried	124:128	arg1	fruits					130:135	coated dried fruits	117:135	coated dried fruits	117:135	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	0	35	theme	novel	24:28	arg1	agent					52:56	novel dehydrating bioactive agent	24:56	novel dehydrating bioactive agent for cut fruit	24:70	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	5	36	theme	antioxidant	723:733	arg1	activity					735:742	improved antioxidant activity	714:742	improved antioxidant activity	714:742	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	2	37	theme	deteriorative	430:442	arg1	processes					444:452	irreversible deteriorative processes	417:452	irreversible deteriorative processes	417:452	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	5	38	theme	dehydration	646:656	arg1	percentage					658:667	dehydration percentage	646:667	dehydration percentage	646:667	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	2	39	theme	dehydrating	308:318	arg1	coatings					247:254	edible coatings	240:254	edible coatings based on pectin and honey	240:280	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	2	39	theme	dehydrating	308:318	arg1	agents					338:343	dehydrating and antimicrobial agents	308:343	dehydrating and antimicrobial agents of cut fruit samples	308:364	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	2	40	theme	irreversible	417:428	arg1	processes					444:452	irreversible deteriorative processes	417:452	irreversible deteriorative processes	417:452	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	5	41	theme	percentage	658:667	arg1	profile					767:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	8	42	theme	novel	1042:1046	arg1	able					1120:1123	able	1120:1123	able	1120:1123	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	8	42	theme	novel	1042:1046	arg1	process					1066:1072	the novel fruit dehydration process	1038:1072	the novel fruit dehydration process	1038:1072	These outcomes suggested that the novel fruit dehydration process, performed by using pectin-honey coating, was able to both preserve the safety and quality of dehydrated fruits, and enhance their authenticity and naturalness.					
29655710	7	43	theme	fractured	919:927	arg1	surface					929:935	fruit fractured surface	913:935	fruit fractured surface	913:935	Finally, morphological analysis performed on fruit fractured surface, highlighted the formation of a non-sticky and homogeneous thin layer.					
29655710	2	44	theme	edible	240:245	arg1	coatings					247:254	edible coatings	240:254	edible coatings based on pectin and honey	240:280	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	2	44	theme	edible	240:245	arg1	agents					338:343	dehydrating and antimicrobial agents	308:343	dehydrating and antimicrobial agents of cut fruit samples	308:364	Specifically, edible coatings based on pectin and honey were prepared and used as dehydrating and antimicrobial agents of cut fruit samples, in this way promoting the fruit preservation from irreversible deteriorative processes.					
29655710	3	45	theme	Pectin-honey	455:466	arg1	coating					468:474	Pectin-honey coating	455:474	Pectin-honey coating	455:474	Pectin-honey coating was tested on apple, cantaloupe melon, mango and pineapple.					
29655710	1	46	theme	fruit	193:197	arg1	dehydration					199:209	the fruit dehydration	189:209	the fruit dehydration	189:209	In this paper, a novel and sustainable process for the fruit dehydration was described.					
29655710	5	47	theme	enriched	670:677	arg1	contents					704:711	enriched polyphenol and vitamin C contents	670:711	enriched polyphenol and vitamin C contents	670:711	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	7	48	theme	layer	1001:1005	arg1	formation					954:962	the formation	950:962	the formation of a non-sticky and homogeneous thin layer	950:1005	Finally, morphological analysis performed on fruit fractured surface, highlighted the formation of a non-sticky and homogeneous thin layer.					
29655710	4	49	theme	uncoated	572:579	arg1	control					600:606	control	600:606	control	600:606	The analysis were performed also on uncoated dehydrated fruits (control).					
29655710	4	49	theme	uncoated	572:579	arg1	fruits					592:597	uncoated dehydrated fruits	572:597	uncoated dehydrated fruits (control)	572:607	The analysis were performed also on uncoated dehydrated fruits (control).					
29655710	5	50	theme	activity	735:742	arg1	profile					767:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile	637:773	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
29655710	7	51	theme	fruit	913:917	arg1	surface					929:935	fruit fractured surface	913:935	fruit fractured surface	913:935	Finally, morphological analysis performed on fruit fractured surface, highlighted the formation of a non-sticky and homogeneous thin layer.					
29655710	0	52	theme	cut	62:64	arg1	fruit					66:70	cut fruit	62:70	cut fruit	62:70	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	0	53	dep	Pectin-honey	0:11	arg1	Enhancement					73:83	Enhancement	73:83	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.	0:136	Pectin-honey coating as novel dehydrating bioactive agent for cut fruit: Enhancement of the functional properties of coated dried fruits.					
29655710	7	54	theme	homogeneous	984:994	arg1	layer					1001:1005	a non-sticky and homogeneous thin layer	967:1005	a non-sticky and homogeneous thin layer	967:1005	Finally, morphological analysis performed on fruit fractured surface, highlighted the formation of a non-sticky and homogeneous thin layer.					
29655710	3	55	theme	cantaloupe	497:506	arg1	melon					508:512	cantaloupe melon	497:512	cantaloupe melon	497:512	Pectin-honey coating was tested on apple, cantaloupe melon, mango and pineapple.					
29655710	5	56	theme	improved	714:721	arg1	activity					735:742	improved antioxidant activity	714:742	improved antioxidant activity	714:742	The coated fruit evidenced enhanced dehydration percentage, enriched polyphenol and vitamin C contents, improved antioxidant activity and volatile molecules profile.					
31761207	1	0	theme	great	171:175	arg1	potential					177:185	the great potential	167:185	the great potential for use as scaffolds in cartilage and bone tissue engineering	167:247	Injectable hydrogels have revealed the great potential for use as scaffolds in cartilage and bone tissue engineering.					
31761207	0	1	theme	antibacterial	79:91	arg1	scaffold					93:100	A biomimetic antibacterial scaffold	66:100	injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration	40:129	Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration.					
31761207	0	2	theme	bone	106:109	arg1	regeneration					118:129	bone tissue regeneration	106:129	bone tissue regeneration	106:129	Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration.					
31761207	3	3	theme	solvent-free	595:606	arg1	medium					608:613	organic solvent-free medium	587:613	organic solvent-free medium	587:613	Spherical nanoparticles of silver were biosynthesized through microwave-assisted green approach using CSE in organic solvent-free medium.					
31761207	2	4	theme	β-tricalcium	308:319	arg1	phosphate					321:329	β-tricalcium phosphate	308:329	β-tricalcium phosphate	308:329	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	5	theme	CSE-Ag	383:388	arg1	extract-nanosilver					363:380	corn silk extract-nanosilver	353:380	corn silk extract-nanosilver (CSE-Ag NPs)	353:393	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	5	theme	CSE-Ag	383:388	arg1	NPs					390:392	CSE-Ag NPs	383:392	CSE-Ag NPs	383:392	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	4	6	theme	thermosensitive	662:676	arg1	hydrogels					678:686	the thermosensitive hydrogels	658:686	the thermosensitive hydrogels	658:686	Rheological experiments demonstrated that the thermosensitive hydrogels have gelification temperature (Tgel) close to body temperature.					
31761207	0	7	theme	tissue	111:116	arg1	regeneration					118:129	bone tissue regeneration	106:129	bone tissue regeneration	106:129	Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration.					
31761207	3	8	theme	organic	587:593	arg1	medium					608:613	organic solvent-free medium	587:613	organic solvent-free medium	587:613	Spherical nanoparticles of silver were biosynthesized through microwave-assisted green approach using CSE in organic solvent-free medium.					
31761207	4	9	contain	have	688:691	arg2	gelification					693:704	gelification	693:704	gelification temperature (Tgel) close to body temperature	693:749	Rheological experiments demonstrated that the thermosensitive hydrogels have gelification temperature (Tgel) close to body temperature.					
31761207	4	9	contain	have	688:691	arg1	hydrogels					678:686	the thermosensitive hydrogels	658:686	the thermosensitive hydrogels	658:686	Rheological experiments demonstrated that the thermosensitive hydrogels have gelification temperature (Tgel) close to body temperature.					
31761207	0	10	theme	acid/corn	11:19	arg1	extract					26:32	Hyaluronic acid/corn silk extract	0:32	Hyaluronic acid/corn silk extract	0:32	Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration.					
31761207	5	11	theme	bone	1051:1054	arg1	differentiation					1056:1070	high bone differentiation	1046:1070	high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration	1046:1174	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	5	12	theme	24 h.	979:983	arg1	cells					1002:1006	24 h. Mesenchymal stem cells	979:1006	24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration	979:1174	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	0	13	theme	Hyaluronic	0:9	arg1	extract					26:32	Hyaluronic acid/corn silk extract	0:32	Hyaluronic acid/corn silk extract	0:32	Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration.					
31761207	4	14	theme	Rheological	616:626	arg1	experiments					628:638	Rheological experiments	616:638	Rheological experiments	616:638	Rheological experiments demonstrated that the thermosensitive hydrogels have gelification temperature (Tgel) close to body temperature.					
31761207	5	15	theme	Mesenchymal	985:995	arg1	cells					1002:1006	24 h. Mesenchymal stem cells	979:1006	24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration	979:1174	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	2	16	theme	regeneration	451:462	arg1	applications					464:475	bone tissue regeneration applications	439:475	bone tissue regeneration applications	439:475	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	17	contain	containing	297:306	arg2	phosphate					321:329	β-tricalcium phosphate	308:329	β-tricalcium phosphate	308:329	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	17	contain	containing	297:306	arg1	hydrogels					287:295	thermosensitive and injectable hydrogels	256:295	thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs)	256:393	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	17	contain	containing	297:306	arg2	acid					343:346	hyaluronic acid	332:346	hyaluronic acid	332:346	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	17	contain	containing	297:306	arg2	extract-nanosilver					363:380	corn silk extract-nanosilver	353:380	corn silk extract-nanosilver (CSE-Ag NPs)	353:393	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	17	contain	containing	297:306	arg2	NPs					390:392	CSE-Ag NPs	383:392	CSE-Ag NPs	383:392	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	18	theme	hyaluronic	332:341	arg1	acid					343:346	hyaluronic acid	332:346	hyaluronic acid	332:346	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	19	theme	bone	439:442	arg1	regeneration					451:462	bone tissue regeneration	439:462	bone tissue regeneration applications	439:475	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	20	theme	tissue	444:449	arg1	regeneration					451:462	bone tissue regeneration	439:462	bone tissue regeneration applications	439:475	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	5	21	theme	antibacterial	789:801	arg1	activity					803:810	antibacterial activity	789:810	antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria	789:944	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	3	22	theme	microwave-assisted	540:557	arg1	approach					565:572	microwave-assisted green approach	540:572	microwave-assisted green approach using CSE in organic solvent-free medium	540:613	Spherical nanoparticles of silver were biosynthesized through microwave-assisted green approach using CSE in organic solvent-free medium.					
31761207	3	23	theme	silver	505:510	arg1	nanoparticles					488:500	Spherical nanoparticles	478:500	Spherical nanoparticles of silver	478:510	Spherical nanoparticles of silver were biosynthesized through microwave-assisted green approach using CSE in organic solvent-free medium.					
31761207	0	24	theme	injectable	40:49	arg1	nanocomposite					51:63	injectable nanocomposite	40:63	injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration	40:129	Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration.					
31761207	2	25	theme	silk	358:361	arg1	extract-nanosilver					363:380	corn silk extract-nanosilver	353:380	corn silk extract-nanosilver (CSE-Ag NPs)	353:393	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	25	theme	silk	358:361	arg1	NPs					390:392	CSE-Ag NPs	383:392	CSE-Ag NPs	383:392	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	3	26	theme	Spherical	478:486	arg1	nanoparticles					488:500	Spherical nanoparticles	478:500	Spherical nanoparticles of silver	478:510	Spherical nanoparticles of silver were biosynthesized through microwave-assisted green approach using CSE in organic solvent-free medium.					
31761207	0	27	theme	silk	21:24	arg1	extract					26:32	Hyaluronic acid/corn silk extract	0:32	Hyaluronic acid/corn silk extract	0:32	Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration.					
31761207	5	28	theme	gram-negative	880:892	arg1	bacteria					937:944	gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria	819:944	bacteria	937:944	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	0	29	dep	nanocomposite	51:63	arg1	scaffold					93:100	A biomimetic antibacterial scaffold	66:100	injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration	40:129	Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration.					
31761207	2	30	theme	potential	422:430	arg1	use					432:434	their potential use	416:434	their potential use in bone tissue regeneration applications	416:475	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	3	31	theme	green	559:563	arg1	approach					565:572	microwave-assisted green approach	540:572	microwave-assisted green approach using CSE in organic solvent-free medium	540:613	Spherical nanoparticles of silver were biosynthesized through microwave-assisted green approach using CSE in organic solvent-free medium.					
31761207	5	32	dep	gram-positive	819:831	arg1	Subtilis					843:850	Bacillus Subtilis	834:850	Bacillus Subtilis	834:850	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	5	32	dep	gram-positive	819:831	arg1	Aureus					868:873	Staphylococcus Aureus	853:873	Staphylococcus Aureus	853:873	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	5	33	contain	containing	764:773	arg1	samples					756:762	The samples	752:762	The samples containing Ag NPs	752:780	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	5	33	contain	containing	764:773	arg2	NPs					778:780	Ag NPs	775:780	Ag NPs	775:780	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	5	34	theme	bone	1151:1154	arg1	regeneration					1163:1174	bone tissue regeneration	1151:1174	bone tissue regeneration	1151:1174	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	1	35	theme	Injectable	132:141	arg1	hydrogels					143:151	Injectable hydrogels	132:151	Injectable hydrogels	132:151	Injectable hydrogels have revealed the great potential for use as scaffolds in cartilage and bone tissue engineering.					
31761207	5	36	dep	bacteria	937:944	arg1	Aeruginosa					907:916	Pseudomonas Aeruginosa	895:916	Pseudomonas Aeruginosa	895:916	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	5	36	dep	bacteria	937:944	arg1	Coli					931:934	Escherichia Coli	919:934	Escherichia Coli	919:934	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	5	37	theme	Ag	775:776	arg1	NPs					778:780	Ag NPs	775:780	Ag NPs	775:780	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	5	38	theme	tissue	1156:1161	arg1	regeneration					1163:1174	bone tissue regeneration	1151:1174	bone tissue regeneration	1151:1174	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	2	39	theme	injectable	276:285	arg1	hydrogels					287:295	thermosensitive and injectable hydrogels	256:295	thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs)	256:393	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	5	40	theme	potential	1128:1136	arg1	scaffold					1138:1145	a potential scaffold	1126:1145	a potential scaffold for bone tissue regeneration	1126:1174	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	2	41	theme	corn	353:356	arg1	extract-nanosilver					363:380	corn silk extract-nanosilver	353:380	corn silk extract-nanosilver (CSE-Ag NPs)	353:393	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	2	41	theme	corn	353:356	arg1	NPs					390:392	CSE-Ag NPs	383:392	CSE-Ag NPs	383:392	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	1	42	theme	tissue	230:235	arg1	engineering					237:247	tissue engineering	230:247	tissue engineering	230:247	Injectable hydrogels have revealed the great potential for use as scaffolds in cartilage and bone tissue engineering.					
31761207	5	43	theme	good	1108:1111	arg1	candidate					1113:1121	a good candidate	1106:1121	a good candidate	1106:1121	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	5	43	theme	good	1108:1111	arg1	thay					1092:1095	thay	1092:1095	thay	1092:1095	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	2	44	from	use	432:434	arg1	applications					464:475	bone tissue regeneration applications	439:475	bone tissue regeneration applications	439:475	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	5	45	theme	high	1046:1049	arg1	differentiation					1056:1070	high bone differentiation	1046:1070	high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration	1046:1174	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	0	46	theme	biomimetic	68:77	arg1	scaffold					93:100	A biomimetic antibacterial scaffold	66:100	injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration	40:129	Hyaluronic acid/corn silk extract based injectable nanocomposite: A biomimetic antibacterial scaffold for bone tissue regeneration.					
31761207	2	47	theme	thermosensitive	256:270	arg1	hydrogels					287:295	thermosensitive and injectable hydrogels	256:295	thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs)	256:393	Here, thermosensitive and injectable hydrogels containing β-tricalcium phosphate, hyaluronic acid, and corn silk extract-nanosilver (CSE-Ag NPs) were synthesized for their potential use in bone tissue regeneration applications.					
31761207	1	48	from	scaffolds	198:206	arg1	cartilage					211:219	cartilage	211:219	cartilage	211:219	Injectable hydrogels have revealed the great potential for use as scaffolds in cartilage and bone tissue engineering.					
31761207	1	48	from	scaffolds	198:206	arg1	bone					225:228	bone	225:228	bone	225:228	Injectable hydrogels have revealed the great potential for use as scaffolds in cartilage and bone tissue engineering.					
31761207	5	49	theme	stem	997:1000	arg1	cells					1002:1006	24 h. Mesenchymal stem cells	979:1006	24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration	979:1174	The samples containing Ag NPs showed antibacterial activity toward gram-positive (Bacillus Subtilis, Staphylococcus Aureus) and gram-negative (Pseudomonas Aeruginosa, Escherichia Coli) bacteria along without cytotoxicity after 24 h. Mesenchymal stem cells seeded in the nanocomposite exhibited high bone differentiation which indicate that thay could be a good candidate as a potential scaffold for bone tissue regeneration.					
31761207	4	50	theme	body	734:737	arg1	temperature					739:749	body temperature	734:749	body temperature	734:749	Rheological experiments demonstrated that the thermosensitive hydrogels have gelification temperature (Tgel) close to body temperature.					
31761207	1	51	dep	cartilage	211:219	arg1	engineering					237:247	tissue engineering	230:247	tissue engineering	230:247	Injectable hydrogels have revealed the great potential for use as scaffolds in cartilage and bone tissue engineering.					
31284277	6	0	theme	biomedical	1051:1060	arg1	implants					1062:1069	other biomedical implants	1045:1069	other biomedical implants	1045:1069	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	3	1	theme	surface	474:480	arg1	morphology					482:491	surface morphology	474:491	surface morphology	474:491	The surface composition, water contact angle, optical and mechanical properties, surface morphology, cell compatibility and protein adhesion were systematically investigated.					
31284277	6	2	theme	surface	999:1005	arg1	modification					1007:1018	surface modification	999:1018	surface modification of artificial cornea and other biomedical implants	999:1069	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	5	3	theme	surface	770:776	arg1	roughness					778:786	surface roughness	770:786	surface roughness	770:786	The modified membranes showed increased hydrophilicity and cytocompatibility, decreased surface roughness and mechanical properties, and suppressed cell and protein adhesion compared to the pristine membrane.					
31284277	0	4	from	modification	12:23	arg1	membrane					46:53	polyvinyl alcohol membrane	28:53	polyvinyl alcohol membrane	28:53	Hydrophilic modification on polyvinyl alcohol membrane by hyaluronic acid.					
31284277	6	5	used	used	991:994	arg2	achievement					907:917	the achievement	903:917	the achievement of the HA coating with anti-adhesive property	903:963	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	2	6	theme	surface	322:328	arg1	hydrophilicity					291:304	the hydrophilicity	287:304	the hydrophilicity of the membrane surface	287:328	Surface modification of PVA membranes by HA molecules was investigated to improve the hydrophilicity of the membrane surface thereby reducing adsorption of the proteins onto the membrane.					
31284277	4	7	theme	ATR-FTIR	568:575	arg1	SEM					591:593	SEM	591:593	SEM	591:593	ATR-FTIR spectra, XPS, SEM and AFM indicated that PVA membranes were successfully modified by grafting of the HA.					
31284277	4	7	theme	ATR-FTIR	568:575	arg1	XPS					586:588	XPS	586:588	XPS	586:588	ATR-FTIR spectra, XPS, SEM and AFM indicated that PVA membranes were successfully modified by grafting of the HA.					
31284277	4	7	theme	ATR-FTIR	568:575	arg1	spectra					577:583	ATR-FTIR spectra	568:583	ATR-FTIR spectra	568:583	ATR-FTIR spectra, XPS, SEM and AFM indicated that PVA membranes were successfully modified by grafting of the HA.					
31284277	4	7	theme	ATR-FTIR	568:575	arg1	AFM					599:601	AFM	599:601	AFM	599:601	ATR-FTIR spectra, XPS, SEM and AFM indicated that PVA membranes were successfully modified by grafting of the HA.					
31284277	6	8	theme	cornea	1034:1039	arg1	modification					1007:1018	surface modification	999:1018	surface modification of artificial cornea and other biomedical implants	999:1069	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	3	9	theme	protein	517:523	arg1	adhesion					525:532	protein adhesion	517:532	protein adhesion	517:532	The surface composition, water contact angle, optical and mechanical properties, surface morphology, cell compatibility and protein adhesion were systematically investigated.					
31284277	2	10	mod	modification	213:224	arg3	Surface					205:211	Surface modification	205:224	Surface modification of PVA membranes by HA molecules	205:257	Surface modification of PVA membranes by HA molecules was investigated to improve the hydrophilicity of the membrane surface thereby reducing adsorption of the proteins onto the membrane.					
31284277	2	10	mod	modification	213:224	arg1	membranes					233:241	PVA membranes	229:241	PVA membranes	229:241	Surface modification of PVA membranes by HA molecules was investigated to improve the hydrophilicity of the membrane surface thereby reducing adsorption of the proteins onto the membrane.					
31284277	4	11	theme	HA	678:679	arg1	grafting					662:669	grafting	662:669	grafting of the HA	662:679	ATR-FTIR spectra, XPS, SEM and AFM indicated that PVA membranes were successfully modified by grafting of the HA.					
31284277	3	12	theme	mechanical	451:460	arg1	properties					462:471	optical and mechanical properties	439:471	optical and mechanical properties	439:471	The surface composition, water contact angle, optical and mechanical properties, surface morphology, cell compatibility and protein adhesion were systematically investigated.					
31284277	6	13	with	achievement	907:917	arg1	property					956:963	anti-adhesive property	942:963	anti-adhesive property	942:963	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	5	14	theme	protein	839:845	arg1	adhesion					847:854	cell and protein adhesion	830:854	adhesion	847:854	The modified membranes showed increased hydrophilicity and cytocompatibility, decreased surface roughness and mechanical properties, and suppressed cell and protein adhesion compared to the pristine membrane.					
31284277	2	15	theme	HA	246:247	arg1	molecules					249:257	HA molecules	246:257	HA molecules	246:257	Surface modification of PVA membranes by HA molecules was investigated to improve the hydrophilicity of the membrane surface thereby reducing adsorption of the proteins onto the membrane.					
31284277	2	16	theme	proteins	365:372	arg1	adsorption					347:356	adsorption	347:356	adsorption of the proteins onto the membrane	347:390	Surface modification of PVA membranes by HA molecules was investigated to improve the hydrophilicity of the membrane surface thereby reducing adsorption of the proteins onto the membrane.					
31284277	1	17	theme	several	143:149	arg1	cycles					163:168	several freeze-thaw cycles	143:168	several freeze-thaw cycles in a mold to produce PVA membrane	143:202	PVA was dissolved in mixed solvent (DMSO and water) and followed by several freeze-thaw cycles in a mold to produce PVA membrane.					
31284277	1	18	theme	freeze-thaw	151:161	arg1	cycles					163:168	several freeze-thaw cycles	143:168	several freeze-thaw cycles in a mold to produce PVA membrane	143:202	PVA was dissolved in mixed solvent (DMSO and water) and followed by several freeze-thaw cycles in a mold to produce PVA membrane.					
31284277	6	19	mod	modification	1007:1018	arg1	implants					1062:1069	other biomedical implants	1045:1069	other biomedical implants	1045:1069	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	6	19	mod	modification	1007:1018	arg3	surface					999:1005	surface modification	999:1018	surface modification of artificial cornea and other biomedical implants	999:1069	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	6	19	mod	modification	1007:1018	arg1	cornea					1034:1039	artificial cornea	1023:1039	artificial cornea	1023:1039	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	3	20	theme	surface	397:403	arg1	composition					405:415	surface composition	397:415	surface composition	397:415	The surface composition, water contact angle, optical and mechanical properties, surface morphology, cell compatibility and protein adhesion were systematically investigated.					
31284277	0	21	theme	Hydrophilic	0:10	arg1	modification					12:23	Hydrophilic modification	0:23	Hydrophilic modification on polyvinyl alcohol membrane by hyaluronic acid	0:72	Hydrophilic modification on polyvinyl alcohol membrane by hyaluronic acid.					
31284277	4	22	mod	modified	650:657	arg3	grafting					662:669	grafting	662:669	grafting of the HA	662:679	ATR-FTIR spectra, XPS, SEM and AFM indicated that PVA membranes were successfully modified by grafting of the HA.					
31284277	4	22	mod	modified	650:657	arg1	membranes					622:630	PVA membranes	618:630	PVA membranes	618:630	ATR-FTIR spectra, XPS, SEM and AFM indicated that PVA membranes were successfully modified by grafting of the HA.					
31284277	0	23	theme	polyvinyl	28:36	arg1	membrane					46:53	polyvinyl alcohol membrane	28:53	polyvinyl alcohol membrane	28:53	Hydrophilic modification on polyvinyl alcohol membrane by hyaluronic acid.					
31284277	1	24	theme	mixed	96:100	arg1	solvent					102:108	mixed solvent	96:108	mixed solvent (DMSO and water)	96:125	PVA was dissolved in mixed solvent (DMSO and water) and followed by several freeze-thaw cycles in a mold to produce PVA membrane.					
31284277	6	25	theme	implants	1062:1069	arg1	modification					1007:1018	surface modification	999:1018	surface modification of artificial cornea and other biomedical implants	999:1069	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	5	26	theme	cell	830:833	arg1	adhesion					847:854	cell and protein adhesion	830:854	adhesion	847:854	The modified membranes showed increased hydrophilicity and cytocompatibility, decreased surface roughness and mechanical properties, and suppressed cell and protein adhesion compared to the pristine membrane.					
31284277	3	27	theme	optical	439:445	arg1	properties					462:471	optical and mechanical properties	439:471	optical and mechanical properties	439:471	The surface composition, water contact angle, optical and mechanical properties, surface morphology, cell compatibility and protein adhesion were systematically investigated.					
31284277	5	28	theme	modified	686:693	arg1	membranes					695:703	The modified membranes	682:703	The modified membranes	682:703	The modified membranes showed increased hydrophilicity and cytocompatibility, decreased surface roughness and mechanical properties, and suppressed cell and protein adhesion compared to the pristine membrane.					
31284277	6	29	theme	other	1045:1049	arg1	implants					1062:1069	other biomedical implants	1045:1069	other biomedical implants	1045:1069	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	2	30	theme	Surface	205:211	arg1	modification					213:224	Surface modification	205:224	Surface modification of PVA membranes by HA molecules	205:257	Surface modification of PVA membranes by HA molecules was investigated to improve the hydrophilicity of the membrane surface thereby reducing adsorption of the proteins onto the membrane.					
31284277	3	31	theme	contact	424:430	arg1	angle					432:436	water contact angle	418:436	water contact angle	418:436	The surface composition, water contact angle, optical and mechanical properties, surface morphology, cell compatibility and protein adhesion were systematically investigated.					
31284277	0	32	theme	alcohol	38:44	arg1	membrane					46:53	polyvinyl alcohol membrane	28:53	polyvinyl alcohol membrane	28:53	Hydrophilic modification on polyvinyl alcohol membrane by hyaluronic acid.					
31284277	6	33	theme	anti-adhesive	942:954	arg1	property					956:963	anti-adhesive property	942:963	anti-adhesive property	942:963	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	5	34	theme	pristine	872:879	arg1	membrane					881:888	the pristine membrane	868:888	the pristine membrane	868:888	The modified membranes showed increased hydrophilicity and cytocompatibility, decreased surface roughness and mechanical properties, and suppressed cell and protein adhesion compared to the pristine membrane.					
31284277	4	35	theme	PVA	618:620	arg1	membranes					622:630	PVA membranes	618:630	PVA membranes	618:630	ATR-FTIR spectra, XPS, SEM and AFM indicated that PVA membranes were successfully modified by grafting of the HA.					
31284277	0	36	theme	hyaluronic	58:67	arg1	acid					69:72	hyaluronic acid	58:72	hyaluronic acid	58:72	Hydrophilic modification on polyvinyl alcohol membrane by hyaluronic acid.					
31284277	2	37	theme	membrane	313:320	arg1	surface					322:328	the membrane surface	309:328	the membrane surface	309:328	Surface modification of PVA membranes by HA molecules was investigated to improve the hydrophilicity of the membrane surface thereby reducing adsorption of the proteins onto the membrane.					
31284277	6	38	theme	coating	929:935	arg1	achievement					907:917	the achievement	903:917	the achievement of the HA coating with anti-adhesive property	903:963	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	6	39	theme	HA	926:927	arg1	coating					929:935	the HA coating	922:935	the HA coating	922:935	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	5	40	dep	showed	705:710	arg1	decreased					760:768	decreased	760:768	decreased surface roughness and mechanical properties	760:812	The modified membranes showed increased hydrophilicity and cytocompatibility, decreased surface roughness and mechanical properties, and suppressed cell and protein adhesion compared to the pristine membrane.					
31284277	5	40	dep	showed	705:710	arg1	suppressed					819:828	suppressed	819:828	suppressed cell and protein adhesion compared to the pristine membrane	819:888	The modified membranes showed increased hydrophilicity and cytocompatibility, decreased surface roughness and mechanical properties, and suppressed cell and protein adhesion compared to the pristine membrane.					
31284277	5	40	dep	showed	705:710	arg1	increased					712:720	increased	712:720	increased hydrophilicity and cytocompatibility	712:757	The modified membranes showed increased hydrophilicity and cytocompatibility, decreased surface roughness and mechanical properties, and suppressed cell and protein adhesion compared to the pristine membrane.					
31284277	2	41	theme	membranes	233:241	arg1	modification					213:224	Surface modification	205:224	Surface modification of PVA membranes by HA molecules	205:257	Surface modification of PVA membranes by HA molecules was investigated to improve the hydrophilicity of the membrane surface thereby reducing adsorption of the proteins onto the membrane.					
31284277	1	42	theme	PVA	191:193	arg1	membrane					195:202	PVA membrane	191:202	PVA membrane	191:202	PVA was dissolved in mixed solvent (DMSO and water) and followed by several freeze-thaw cycles in a mold to produce PVA membrane.					
31284277	2	43	theme	PVA	229:231	arg1	membranes					233:241	PVA membranes	229:241	PVA membranes	229:241	Surface modification of PVA membranes by HA molecules was investigated to improve the hydrophilicity of the membrane surface thereby reducing adsorption of the proteins onto the membrane.					
31284277	1	44	from	cycles	163:168	arg1	mold					175:178	a mold	173:178	a mold to produce PVA membrane	173:202	PVA was dissolved in mixed solvent (DMSO and water) and followed by several freeze-thaw cycles in a mold to produce PVA membrane.					
31284277	3	45	theme	cell	494:497	arg1	compatibility					499:511	cell compatibility	494:511	cell compatibility	494:511	The surface composition, water contact angle, optical and mechanical properties, surface morphology, cell compatibility and protein adhesion were systematically investigated.					
31284277	6	46	theme	artificial	1023:1032	arg1	cornea					1034:1039	artificial cornea	1023:1039	artificial cornea	1023:1039	In general, the achievement of the HA coating with anti-adhesive property can potentially be widely used on surface modification of artificial cornea and other biomedical implants.					
31284277	3	47	theme	water	418:422	arg1	angle					432:436	water contact angle	418:436	water contact angle	418:436	The surface composition, water contact angle, optical and mechanical properties, surface morphology, cell compatibility and protein adhesion were systematically investigated.					
31284277	5	48	theme	mechanical	792:801	arg1	properties					803:812	mechanical properties	792:812	mechanical properties	792:812	The modified membranes showed increased hydrophilicity and cytocompatibility, decreased surface roughness and mechanical properties, and suppressed cell and protein adhesion compared to the pristine membrane.					
31284277	1	49	dep	solvent	102:108	arg1	water					120:124	water	120:124	water	120:124	PVA was dissolved in mixed solvent (DMSO and water) and followed by several freeze-thaw cycles in a mold to produce PVA membrane.					
31284277	1	49	dep	solvent	102:108	arg1	DMSO					111:114	DMSO	111:114	DMSO	111:114	PVA was dissolved in mixed solvent (DMSO and water) and followed by several freeze-thaw cycles in a mold to produce PVA membrane.					
31055150	6	0	theme	closure	868:874	arg1	rates					876:880	wound closure rates	862:880	wound closure rates	862:880	Furthermore, compared with conventional dressings, wound healing test revealed that CT/PVA/IT hydrogel could accelerated wound healing in vivo, wound closure rates reached 95.5% after 10 days.					
31055150	3	1	theme	dressing	469:476	arg1	structure					434:442	The structure	430:442	The structure of the prepared hydrogel dressing	430:476	The structure of the prepared hydrogel dressing was characterized by FTIR, XPS, XRD, SEM and TEM.					
31055150	6	2	theme	wound	862:866	arg1	rates					876:880	wound closure rates	862:880	wound closure rates	862:880	Furthermore, compared with conventional dressings, wound healing test revealed that CT/PVA/IT hydrogel could accelerated wound healing in vivo, wound closure rates reached 95.5% after 10 days.					
31055150	6	3	theme	wound	769:773	arg1	healing					775:781	wound healing	769:781	wound healing test	769:786	Furthermore, compared with conventional dressings, wound healing test revealed that CT/PVA/IT hydrogel could accelerated wound healing in vivo, wound closure rates reached 95.5% after 10 days.					
31055150	1	4	theme	promising	228:236	arg1	material					238:245	a promising material	226:245	a promising material for wound dressings	226:265	A novel antibacterial hydrogel was prepared through the addition of IT to a chitin (CT) and polyvinyl alcohol (PVA) hydrogel, creating a promising material for wound dressings.					
31055150	5	5	theme	Cytotoxicity	620:631	arg1	cells					656:660	L929 fibroblast cells	640:660	L929 fibroblast cells	640:660	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	5	5	theme	Cytotoxicity	620:631	arg1	tests					633:637	Cytotoxicity tests	620:637	Cytotoxicity tests (L929 fibroblast cells)	620:661	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	4	6	theme	composite	532:540	arg1	hydrogel					542:549	The composite hydrogel	528:549	The composite hydrogel	528:549	The composite hydrogel exhibited excellent anti-bacterial activity under the visible light.					
31055150	2	7	theme	particles	289:297	arg1	addition					272:279	The addition	268:279	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel	268:348	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel as well as had a positive impact on the mechanical properties of the hydrogels.					
31055150	2	8	theme	anti-bacterial	314:327	arg1	activity					329:336	the anti-bacterial activity	310:336	the anti-bacterial activity of hydrogel	310:348	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel as well as had a positive impact on the mechanical properties of the hydrogels.					
31055150	6	9	theme	conventional	745:756	arg1	dressings					758:766	conventional dressings	745:766	conventional dressings	745:766	Furthermore, compared with conventional dressings, wound healing test revealed that CT/PVA/IT hydrogel could accelerated wound healing in vivo, wound closure rates reached 95.5% after 10 days.					
31055150	7	10	contain	has	955:957	arg1	hydrogel					946:953	the novel hydrogel	936:953	the novel hydrogel	936:953	This study suggests that the novel hydrogel has considerable potential for applications in wound dressings.					
31055150	7	10	contain	has	955:957	arg2	potential					972:980	considerable potential	959:980	considerable potential for applications in wound dressings	959:1016	This study suggests that the novel hydrogel has considerable potential for applications in wound dressings.					
31055150	1	11	theme	novel	93:97	arg1	hydrogel					113:120	A novel antibacterial hydrogel	91:120	A novel antibacterial hydrogel	91:120	A novel antibacterial hydrogel was prepared through the addition of IT to a chitin (CT) and polyvinyl alcohol (PVA) hydrogel, creating a promising material for wound dressings.					
31055150	0	12	theme	antibacterial	15:27	arg1	agent					29:33	Photocatalytic antibacterial agent	0:33	Photocatalytic antibacterial agent	0:33	Photocatalytic antibacterial agent incorporated double-network hydrogel for wound healing.					
31055150	6	13	theme	wound	839:843	arg1	healing					845:851	wound healing	839:851	wound healing	839:851	Furthermore, compared with conventional dressings, wound healing test revealed that CT/PVA/IT hydrogel could accelerated wound healing in vivo, wound closure rates reached 95.5% after 10 days.					
31055150	2	14	theme	nano	284:287	arg1	particles					289:297	nano particles	284:297	nano particles IT endowed the anti-bacterial activity of hydrogel	284:348	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel as well as had a positive impact on the mechanical properties of the hydrogels.					
31055150	1	15	theme	antibacterial	99:111	arg1	hydrogel					113:120	A novel antibacterial hydrogel	91:120	A novel antibacterial hydrogel	91:120	A novel antibacterial hydrogel was prepared through the addition of IT to a chitin (CT) and polyvinyl alcohol (PVA) hydrogel, creating a promising material for wound dressings.					
31055150	5	16	dep	80	698:699	arg1	to					695:696	to	695:696	to	695:696	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	0	17	theme	Photocatalytic	0:13	arg1	agent					29:33	Photocatalytic antibacterial agent	0:33	Photocatalytic antibacterial agent	0:33	Photocatalytic antibacterial agent incorporated double-network hydrogel for wound healing.					
31055150	1	18	theme	wound	251:255	arg1	dressings					257:265	wound dressings	251:265	wound dressings	251:265	A novel antibacterial hydrogel was prepared through the addition of IT to a chitin (CT) and polyvinyl alcohol (PVA) hydrogel, creating a promising material for wound dressings.					
31055150	1	19	dep	chitin	167:172	arg1	hydrogel					207:214	hydrogel	207:214	hydrogel	207:214	A novel antibacterial hydrogel was prepared through the addition of IT to a chitin (CT) and polyvinyl alcohol (PVA) hydrogel, creating a promising material for wound dressings.					
31055150	5	20	dep	viability	707:715	arg1	%					700:700	%	700:700	%	700:700	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	1	21	theme	polyvinyl	183:191	arg1	alcohol					193:199	polyvinyl alcohol	183:199	polyvinyl alcohol (PVA)	183:205	A novel antibacterial hydrogel was prepared through the addition of IT to a chitin (CT) and polyvinyl alcohol (PVA) hydrogel, creating a promising material for wound dressings.					
31055150	1	21	theme	polyvinyl	183:191	arg1	PVA					202:204	PVA	202:204	PVA	202:204	A novel antibacterial hydrogel was prepared through the addition of IT to a chitin (CT) and polyvinyl alcohol (PVA) hydrogel, creating a promising material for wound dressings.					
31055150	2	22	theme	mechanical	390:399	arg1	properties					401:410	the mechanical properties	386:410	the mechanical properties of the hydrogels	386:427	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel as well as had a positive impact on the mechanical properties of the hydrogels.					
31055150	7	23	theme	wound	1002:1006	arg1	dressings					1008:1016	wound dressings	1002:1016	wound dressings	1002:1016	This study suggests that the novel hydrogel has considerable potential for applications in wound dressings.					
31055150	5	24	dep	%	700:700	arg1	80					698:699	80	698:699	80	698:699	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	4	25	theme	visible	605:611	arg1	light					613:617	the visible light	601:617	the visible light	601:617	The composite hydrogel exhibited excellent anti-bacterial activity under the visible light.					
31055150	0	26	theme	double-network	48:61	arg1	hydrogel					63:70	double-network hydrogel	48:70	double-network hydrogel	48:70	Photocatalytic antibacterial agent incorporated double-network hydrogel for wound healing.					
31055150	7	27	from	applications	986:997	arg1	dressings					1008:1016	wound dressings	1002:1016	wound dressings	1002:1016	This study suggests that the novel hydrogel has considerable potential for applications in wound dressings.					
31055150	2	28	theme	positive	367:374	arg1	impact					376:381	a positive impact	365:381	a positive impact	365:381	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel as well as had a positive impact on the mechanical properties of the hydrogels.					
31055150	0	29	theme	wound	76:80	arg1	healing					82:88	wound healing	76:88	wound healing	76:88	Photocatalytic antibacterial agent incorporated double-network hydrogel for wound healing.					
31055150	6	30	theme	CT/PVA/IT	802:810	arg1	hydrogel					812:819	CT/PVA/IT hydrogel	802:819	CT/PVA/IT hydrogel	802:819	Furthermore, compared with conventional dressings, wound healing test revealed that CT/PVA/IT hydrogel could accelerated wound healing in vivo, wound closure rates reached 95.5% after 10 days.					
31055150	7	31	theme	considerable	959:970	arg1	potential					972:980	considerable potential	959:980	considerable potential for applications in wound dressings	959:1016	This study suggests that the novel hydrogel has considerable potential for applications in wound dressings.					
31055150	3	32	theme	prepared	451:458	arg1	dressing					469:476	the prepared hydrogel dressing	447:476	the prepared hydrogel dressing	447:476	The structure of the prepared hydrogel dressing was characterized by FTIR, XPS, XRD, SEM and TEM.					
31055150	2	33	theme	hydrogels	419:427	arg1	properties					401:410	the mechanical properties	386:410	the mechanical properties of the hydrogels	386:427	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel as well as had a positive impact on the mechanical properties of the hydrogels.					
31055150	6	34	dep	accelerated	827:837	arg1	could					821:825	could	821:825	could	821:825	Furthermore, compared with conventional dressings, wound healing test revealed that CT/PVA/IT hydrogel could accelerated wound healing in vivo, wound closure rates reached 95.5% after 10 days.					
31055150	5	35	theme	L929	640:643	arg1	cells					656:660	L929 fibroblast cells	640:660	L929 fibroblast cells	640:660	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	5	35	theme	L929	640:643	arg1	tests					633:637	Cytotoxicity tests	620:637	Cytotoxicity tests (L929 fibroblast cells)	620:661	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	7	36	theme	novel	940:944	arg1	hydrogel					946:953	the novel hydrogel	936:953	the novel hydrogel	936:953	This study suggests that the novel hydrogel has considerable potential for applications in wound dressings.					
31055150	3	37	theme	hydrogel	460:467	arg1	dressing					469:476	the prepared hydrogel dressing	447:476	the prepared hydrogel dressing	447:476	The structure of the prepared hydrogel dressing was characterized by FTIR, XPS, XRD, SEM and TEM.					
31055150	5	38	theme	fibroblast	645:654	arg1	cells					656:660	L929 fibroblast cells	640:660	L929 fibroblast cells	640:660	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	5	38	theme	fibroblast	645:654	arg1	tests					633:637	Cytotoxicity tests	620:637	Cytotoxicity tests (L929 fibroblast cells)	620:661	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	2	39	dep	particles	289:297	arg1	IT					299:300	IT	299:300	IT	299:300	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel as well as had a positive impact on the mechanical properties of the hydrogels.					
31055150	2	39	dep	particles	289:297	arg1	endowed					302:308	endowed	302:308	endowed	302:308	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel as well as had a positive impact on the mechanical properties of the hydrogels.					
31055150	6	40	theme	healing	775:781	arg1	test					783:786	wound healing test	769:786	wound healing test	769:786	Furthermore, compared with conventional dressings, wound healing test revealed that CT/PVA/IT hydrogel could accelerated wound healing in vivo, wound closure rates reached 95.5% after 10 days.					
31055150	1	41	theme	IT	159:160	arg1	addition					147:154	the addition	143:154	the addition of IT to a chitin (CT) and polyvinyl alcohol (PVA) hydrogel	143:214	A novel antibacterial hydrogel was prepared through the addition of IT to a chitin (CT) and polyvinyl alcohol (PVA) hydrogel, creating a promising material for wound dressings.					
31055150	4	42	theme	anti-bacterial	571:584	arg1	activity					586:593	excellent anti-bacterial activity	561:593	excellent anti-bacterial activity	561:593	The composite hydrogel exhibited excellent anti-bacterial activity under the visible light.					
31055150	5	43	theme	cell	702:705	arg1	viability					707:715	up to 80% cell viability	692:715	up to 80% cell viability	692:715	Cytotoxicity tests (L929 fibroblast cells) showed all samples achieving up to 80% cell viability.					
31055150	2	44	theme	hydrogel	341:348	arg1	activity					329:336	the anti-bacterial activity	310:336	the anti-bacterial activity of hydrogel	310:348	The addition of nano particles IT endowed the anti-bacterial activity of hydrogel as well as had a positive impact on the mechanical properties of the hydrogels.					
31055150	4	45	theme	excellent	561:569	arg1	activity					586:593	excellent anti-bacterial activity	561:593	excellent anti-bacterial activity	561:593	The composite hydrogel exhibited excellent anti-bacterial activity under the visible light.					
30640523	17	0	theme	predifferentiated	2672:2688	arg1	MSCs					2690:2693	chondrogenic predifferentiated MSCs	2659:2693	chondrogenic predifferentiated MSCs	2659:2693	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	7	1	from	defects	1097:1103	arg1	knees					1118:1122	the right knees	1108:1122	the right knees	1108:1122	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	10	2	theme	overall	1513:1519	arg1	repair					1540:1545	overall superior cartilage repair	1513:1545	overall superior cartilage repair	1513:1545	RESULTS Transplanting undiff-MSCs resulted in overall superior cartilage repair as compared with chondro-MSCs, HA alone, or no treatment.					
30640523	6	3	theme	osteochondral	880:892	arg1	defects					894:900	Critical-sized osteochondral defects	865:900	Critical-sized osteochondral defects	865:900	METHODS Critical-sized osteochondral defects were created in the trochlear grooves of rat femurs.					
30640523	11	4	theme	sites	1642:1646	arg1	surfaces					1619:1626	The articular surfaces	1605:1626	The articular surfaces of the defect sites in the undiff-MSC group	1605:1670	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	11	4	theme	sites	1642:1646	arg1	smoother					1688:1695	smoother	1688:1695	smoother	1688:1695	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	9	5	theme	cartilage	1403:1411	arg1	repair					1413:1418	The cartilage repair	1399:1418	The cartilage repair	1399:1418	The cartilage repair was evaluated at 8 and 16 weeks after surgery.					
30640523	12	6	theme	undiff-MSC	1737:1746	arg1	group					1748:1752	The undiff-MSC group	1733:1752	The undiff-MSC group	1733:1752	The undiff-MSC group showed cellular morphology and arrangement similar to surrounding normal articular cartilage tissue at 16 weeks, both of which were also better than those of the other groups.					
30640523	8	7	theme	4	1255:1255	arg1	%					1256:1256	%	1256:1256	%	1256:1256	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	12	8	theme	other	1916:1920	arg1	groups					1922:1927	the other groups	1912:1927	the other groups	1912:1927	The undiff-MSC group showed cellular morphology and arrangement similar to surrounding normal articular cartilage tissue at 16 weeks, both of which were also better than those of the other groups.					
30640523	7	9	theme	undifferentiated	1133:1148	arg1	undiff-MSCs					1161:1171	undiff-MSCs	1161:1171	undiff-MSCs	1161:1171	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	7	9	theme	undifferentiated	1133:1148	arg1	hUCB-MSCs					1150:1158	undifferentiated hUCB-MSCs	1133:1158	undifferentiated hUCB-MSCs (undiff-MSCs)	1133:1172	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	8	10	from	untreated	1377:1385	arg1	rats					1393:1396	20 rats	1390:1396	20 rats	1390:1396	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	1	11	theme	umbilical	186:194	arg1	hUCB-MSCs					239:247	hUCB-MSCs	239:247	hUCB-MSCs	239:247	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	11	theme	umbilical	186:194	arg1	cells					232:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells	169:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs)	169:248	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	6	12	theme	rat	943:945	arg1	femurs					947:952	rat femurs	943:952	rat femurs	943:952	METHODS Critical-sized osteochondral defects were created in the trochlear grooves of rat femurs.					
30640523	17	13	theme	cell	2706:2709	arg1	strategy					2719:2726	a stem cell therapy strategy	2699:2726	a stem cell therapy strategy for cartilage repair	2699:2747	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	17	13	theme	cell	2706:2709	arg1	use					2617:2619	the use	2613:2619	the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs	2613:2693	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	4	14	theme	transplanting	680:692	arg1	MSCs					773:776	MSCs	773:776	MSCs	773:776	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	4	14	theme	transplanting	680:692	arg1	cells					766:770	predifferentiated mesenchymal stem cells	731:770	transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs)	680:777	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	1	15	theme	blood-derived	201:213	arg1	hUCB-MSCs					239:247	hUCB-MSCs	239:247	hUCB-MSCs	239:247	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	15	theme	blood-derived	201:213	arg1	cells					232:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells	169:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs)	169:248	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	7	16	theme	right	1112:1116	arg1	knees					1118:1122	the right knees	1108:1122	the right knees	1108:1122	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	1	17	theme	stem	227:230	arg1	hUCB-MSCs					239:247	hUCB-MSCs	239:247	hUCB-MSCs	239:247	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	17	theme	stem	227:230	arg1	cells					232:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells	169:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs)	169:248	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	17	18	theme	undifferentiated	2624:2639	arg1	MSCs					2641:2644	undifferentiated MSCs	2624:2644	undifferentiated MSCs	2624:2644	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	15	19	theme	rat	2422:2424	arg1	model					2426:2430	a rat model	2420:2430	a rat model	2420:2430	CONCLUSION This study demonstrated that treatment with undiff-MSCs resulted in more favorable cartilage repair than that with chondro-MSCs in a rat model.					
30640523	14	20	theme	histological	2120:2131	arg1	scores					2133:2138	The histological scores	2116:2138	The histological scores	2116:2138	The histological scores also revealed that cartilage repair with undiff-MSCs was better than that with chondro-MSCs, HA alone, or no treatment ( P < .05 in all).					
30640523	13	21	theme	immunohistochemical	2086:2104	arg1	staining					2106:2113	immunohistochemical staining	2086:2113	immunohistochemical staining	2086:2113	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	11	22	from	sites	1642:1646	arg1	group					1666:1670	the undiff-MSC group	1651:1670	the undiff-MSC group	1651:1670	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	4	23	theme	stem	761:764	arg1	MSCs					773:776	MSCs	773:776	MSCs	773:776	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	4	23	theme	stem	761:764	arg1	cells					766:770	predifferentiated mesenchymal stem cells	731:770	transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs)	680:777	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	0	24	theme	Rat	158:160	arg1	Model					162:166	a Rat Model	156:166	a Rat Model	156:166	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	13	25	theme	safranin	2054:2061	arg1	O					2063:2063	safranin O	2054:2063	safranin O	2054:2063	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	3	26	theme	chondrogenic	574:585	arg1	predifferentiation					587:604	chondrogenic predifferentiation	574:604	chondrogenic predifferentiation of hUCB-MSCs	574:617	Moreover, little study has been conducted on the effects of chondrogenic predifferentiation of hUCB-MSCs on cartilage repair.					
30640523	7	27	theme	acid	1056:1059	arg1	hydrogel					1066:1073	4% hyaluronic acid (HA) hydrogel	1042:1073	4% hyaluronic acid (HA) hydrogel	1042:1073	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	11	28	theme	other	1715:1719	arg1	treatments					1721:1730	the other treatments	1711:1730	the other treatments	1711:1730	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	7	29	theme	%	1043:1043	arg1	hydrogel					1066:1073	4% hyaluronic acid (HA) hydrogel	1042:1073	4% hyaluronic acid (HA) hydrogel	1042:1073	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	2	30	theme	cartilage	496:504	arg1	repair					506:511	cartilage repair	496:511	cartilage repair	496:511	However, few in vivo studies have reported the use of hUCB-MSCs on cartilage repair.					
30640523	17	31	theme	study	2599:2603	arg1	results					2583:2589	The results	2579:2589	The results of this study	2579:2603	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	8	32	theme	right	1320:1324	arg1	knees					1326:1330	the right knees	1316:1330	the right knees	1316:1330	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	3	33	from	effects	563:569	arg1	repair					632:637	cartilage repair	622:637	cartilage repair	622:637	Moreover, little study has been conducted on the effects of chondrogenic predifferentiation of hUCB-MSCs on cartilage repair.					
30640523	0	34	theme	Cells	86:90	arg1	Comparison					0:9	Comparison	0:9	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	0:90	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	2	35	theme	hUCB-MSCs	483:491	arg1	use					476:478	the use	472:478	the use of hUCB-MSCs on cartilage repair	472:511	However, few in vivo studies have reported the use of hUCB-MSCs on cartilage repair.					
30640523	4	36	theme	predifferentiated	731:747	arg1	MSCs					773:776	MSCs	773:776	MSCs	773:776	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	4	36	theme	predifferentiated	731:747	arg1	cells					766:770	predifferentiated mesenchymal stem cells	731:770	transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs)	680:777	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	14	37	with	repair	2169:2174	arg1	undiff-MSCs					2181:2191	undiff-MSCs	2181:2191	undiff-MSCs	2181:2191	The histological scores also revealed that cartilage repair with undiff-MSCs was better than that with chondro-MSCs, HA alone, or no treatment ( P < .05 in all).					
30640523	5	38	theme	STUDY	815:819	arg1	DESIGN					821:826	STUDY DESIGN	815:826	STUDY DESIGN	815:826	STUDY DESIGN Controlled laboratory study.					
30640523	15	39	theme	cartilage	2372:2380	arg1	repair					2382:2387	more favorable cartilage repair	2357:2387	more favorable cartilage repair	2357:2387	CONCLUSION This study demonstrated that treatment with undiff-MSCs resulted in more favorable cartilage repair than that with chondro-MSCs in a rat model.					
30640523	1	40	theme	BACKGROUND	169:178	arg1	hUCB-MSCs					239:247	hUCB-MSCs	239:247	hUCB-MSCs	239:247	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	40	theme	BACKGROUND	169:178	arg1	cells					232:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells	169:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs)	169:248	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	16	41	theme	cartilage	2542:2550	arg1	repair					2552:2557	cartilage repair	2542:2557	cartilage repair	2542:2557	These findings indicate that chondrogenic predifferentiation of MSCs before transplantation does not enhance cartilage repair.					
30640523	0	42	theme	Umbilical	111:119	arg1	Blood					126:130	Human Umbilical Cord Blood	105:130	Human Umbilical Cord Blood	105:130	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	13	43	theme	normal	2005:2010	arg1	tissue					2032:2037	surrounding normal articular cartilage tissue	1993:2037	surrounding normal articular cartilage tissue	1993:2037	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	7	44	theme	hUCB-MSCs	1013:1021	arg1	composite					969:977	a composite	967:977	a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel	967:1073	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	7	44	theme	hUCB-MSCs	1013:1021	arg1	hydrogel					1066:1073	4% hyaluronic acid (HA) hydrogel	1042:1073	4% hyaluronic acid (HA) hydrogel	1042:1073	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	7	44	theme	hUCB-MSCs	1013:1021	arg1	hUCB-MSCs					1013:1021	chondrogenic predifferentiated hUCB-MSCs	982:1021	chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs)	982:1036	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	11	45	from	group	1666:1670	arg1	surfaces					1619:1626	The articular surfaces	1605:1626	The articular surfaces of the defect sites in the undiff-MSC group	1605:1670	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	11	45	from	group	1666:1670	arg1	smoother					1688:1695	smoother	1688:1695	smoother	1688:1695	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	7	46	theme	left	1220:1223	arg1	knees					1225:1229	the left knees	1216:1229	the left knees	1216:1229	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	1	47	theme	high	380:383	arg1	capacity					395:402	high expansion capacity	380:402	high expansion capacity	380:402	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	0	48	theme	Predifferentiated	51:67	arg1	Cells					86:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	7	49	theme	chondrogenic	982:993	arg1	chondro-MSCs					1024:1035	chondro-MSCs	1024:1035	chondro-MSCs	1024:1035	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	7	49	theme	chondrogenic	982:993	arg1	hUCB-MSCs					1013:1021	chondrogenic predifferentiated hUCB-MSCs	982:1021	chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs)	982:1036	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	8	50	theme	left	1356:1359	arg1	knees					1361:1365	the left knees	1352:1365	the left knees	1352:1365	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	1	51	theme	promising	281:289	arg1	source					296:301	a promising cell source	279:301	a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity	279:426	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	51	theme	promising	281:289	arg1	interest					267:274	much interest	262:274	much interest	262:274	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	2	52	theme	in	442:443	arg1	studies					450:456	few in vivo studies	438:456	few in vivo studies	438:456	However, few in vivo studies have reported the use of hUCB-MSCs on cartilage repair.					
30640523	17	53	theme	CLINICAL	2560:2567	arg1	RELEVANCE					2569:2577	CLINICAL RELEVANCE	2560:2577	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.	2560:2748	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	15	54	with	treatment	2318:2326	arg1	undiff-MSCs					2333:2343	undiff-MSCs	2333:2343	undiff-MSCs	2333:2343	CONCLUSION This study demonstrated that treatment with undiff-MSCs resulted in more favorable cartilage repair than that with chondro-MSCs in a rat model.					
30640523	0	55	theme	Undifferentiated	14:29	arg1	Cells					86:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	0	56	theme	Chondrogenic	38:49	arg1	Cells					86:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	1	57	theme	regenerative	307:318	arg1	medicine					320:327	regenerative medicine	307:327	regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity	307:426	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	7	58	theme	HA	1181:1182	arg1	hydrogel					1184:1191	4% HA hydrogel	1178:1191	4% HA hydrogel	1178:1191	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	6	59	theme	trochlear	922:930	arg1	grooves					932:938	the trochlear grooves	918:938	the trochlear grooves of rat femurs	918:952	METHODS Critical-sized osteochondral defects were created in the trochlear grooves of rat femurs.					
30640523	12	60	theme	similar	1797:1803	arg1	morphology					1770:1779	cellular morphology	1761:1779	cellular morphology	1761:1779	The undiff-MSC group showed cellular morphology and arrangement similar to surrounding normal articular cartilage tissue at 16 weeks, both of which were also better than those of the other groups.					
30640523	11	61	from	surfaces	1619:1626	arg1	group					1666:1670	the undiff-MSC group	1651:1670	the undiff-MSC group	1651:1670	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	11	62	theme	undiff-MSC	1655:1664	arg1	group					1666:1670	the undiff-MSC group	1651:1670	the undiff-MSC group	1651:1670	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	13	63	theme	similar	1982:1988	arg1	coloration					1971:1980	coloration	1971:1980	coloration similar to surrounding normal articular cartilage tissue	1971:2037	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	7	64	theme	4	1178:1178	arg1	%					1179:1179	%	1179:1179	%	1179:1179	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	12	65	theme	normal	1820:1825	arg1	tissue					1847:1852	surrounding normal articular cartilage tissue	1808:1852	surrounding normal articular cartilage tissue	1808:1852	The undiff-MSC group showed cellular morphology and arrangement similar to surrounding normal articular cartilage tissue at 16 weeks, both of which were also better than those of the other groups.					
30640523	12	66	theme	surrounding	1808:1818	arg1	tissue					1847:1852	surrounding normal articular cartilage tissue	1808:1852	surrounding normal articular cartilage tissue	1808:1852	The undiff-MSC group showed cellular morphology and arrangement similar to surrounding normal articular cartilage tissue at 16 weeks, both of which were also better than those of the other groups.					
30640523	16	67	theme	chondrogenic	2462:2473	arg1	predifferentiation					2475:2492	chondrogenic predifferentiation	2462:2492	chondrogenic predifferentiation of MSCs before transplantation	2462:2523	These findings indicate that chondrogenic predifferentiation of MSCs before transplantation does not enhance cartilage repair.					
30640523	13	68	theme	surrounding	1993:2003	arg1	tissue					2032:2037	surrounding normal articular cartilage tissue	1993:2037	surrounding normal articular cartilage tissue	1993:2037	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	8	69	from	defects	1341:1347	arg1	knees					1361:1365	the left knees	1352:1365	the left knees	1352:1365	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	12	70	theme	cartilage	1837:1845	arg1	tissue					1847:1852	surrounding normal articular cartilage tissue	1808:1852	surrounding normal articular cartilage tissue	1808:1852	The undiff-MSC group showed cellular morphology and arrangement similar to surrounding normal articular cartilage tissue at 16 weeks, both of which were also better than those of the other groups.					
30640523	12	71	theme	cellular	1761:1768	arg1	morphology					1770:1779	cellular morphology	1761:1779	cellular morphology	1761:1779	The undiff-MSC group showed cellular morphology and arrangement similar to surrounding normal articular cartilage tissue at 16 weeks, both of which were also better than those of the other groups.					
30640523	13	72	theme	undiff-MSC	1947:1956	arg1	group					1958:1962	the undiff-MSC group	1943:1962	the undiff-MSC group	1943:1962	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	0	73	theme	Stem	81:84	arg1	Cells					86:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	10	74	theme	superior	1521:1528	arg1	repair					1540:1545	overall superior cartilage repair	1513:1545	overall superior cartilage repair	1513:1545	RESULTS Transplanting undiff-MSCs resulted in overall superior cartilage repair as compared with chondro-MSCs, HA alone, or no treatment.					
30640523	1	75	theme	much	262:265	arg1	source					296:301	a promising cell source	279:301	a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity	279:426	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	75	theme	much	262:265	arg1	interest					267:274	much interest	262:274	much interest	262:274	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	17	76	theme	MSCs	2690:2693	arg1	use					2617:2619	the use	2613:2619	the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs	2613:2693	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	17	76	theme	MSCs	2690:2693	arg1	strategy					2719:2726	a stem cell therapy strategy	2699:2726	a stem cell therapy strategy for cartilage repair	2699:2747	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	14	77	from	<	2263:2263	arg1	all					2272:2274	all	2272:2274	all	2272:2274	The histological scores also revealed that cartilage repair with undiff-MSCs was better than that with chondro-MSCs, HA alone, or no treatment ( P < .05 in all).					
30640523	11	78	theme	articular	1609:1617	arg1	surfaces					1619:1626	The articular surfaces	1605:1626	The articular surfaces of the defect sites in the undiff-MSC group	1605:1670	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	11	78	theme	articular	1609:1617	arg1	smoother					1688:1695	smoother	1688:1695	smoother	1688:1695	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	6	79	theme	Critical-sized	865:878	arg1	defects					894:900	Critical-sized osteochondral defects	865:900	Critical-sized osteochondral defects	865:900	METHODS Critical-sized osteochondral defects were created in the trochlear grooves of rat femurs.					
30640523	13	80	dep	O	2063:2063	arg1	staining					2106:2113	immunohistochemical staining	2086:2113	immunohistochemical staining	2086:2113	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	7	81	dep	transplanted	1079:1090	arg1	whereas					1125:1131	whereas	1125:1131	whereas	1125:1131	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	3	82	theme	little	524:529	arg1	study					531:535	little study	524:535	little study	524:535	Moreover, little study has been conducted on the effects of chondrogenic predifferentiation of hUCB-MSCs on cartilage repair.					
30640523	8	83	theme	HA	1258:1259	arg1	hydrogel					1261:1268	4% HA hydrogel	1255:1268	4% HA hydrogel without MSCs	1255:1281	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	1	84	theme	Human	180:184	arg1	hUCB-MSCs					239:247	hUCB-MSCs	239:247	hUCB-MSCs	239:247	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	84	theme	Human	180:184	arg1	cells					232:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells	169:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs)	169:248	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	0	85	theme	Mesenchymal	69:79	arg1	Cells					86:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells	14:90	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	6	86	theme	femurs	947:952	arg1	grooves					932:938	the trochlear grooves	918:938	the trochlear grooves of rat femurs	918:952	METHODS Critical-sized osteochondral defects were created in the trochlear grooves of rat femurs.					
30640523	17	87	theme	stem	2701:2704	arg1	strategy					2719:2726	a stem cell therapy strategy	2699:2726	a stem cell therapy strategy for cartilage repair	2699:2747	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	17	87	theme	stem	2701:2704	arg1	use					2617:2619	the use	2613:2619	the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs	2613:2693	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	14	88	theme	cartilage	2159:2167	arg1	repair					2169:2174	cartilage repair	2159:2174	cartilage repair with undiff-MSCs	2159:2191	The histological scores also revealed that cartilage repair with undiff-MSCs was better than that with chondro-MSCs, HA alone, or no treatment ( P < .05 in all).					
30640523	11	89	theme	defect	1635:1640	arg1	sites					1642:1646	the defect sites	1631:1646	the defect sites in the undiff-MSC group	1631:1670	The articular surfaces of the defect sites in the undiff-MSC group were relatively smoother than those of the other treatments.					
30640523	1	90	theme	cord	196:199	arg1	hUCB-MSCs					239:247	hUCB-MSCs	239:247	hUCB-MSCs	239:247	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	90	theme	cord	196:199	arg1	cells					232:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells	169:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs)	169:248	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	17	91	theme	therapy	2711:2717	arg1	strategy					2719:2726	a stem cell therapy strategy	2699:2726	a stem cell therapy strategy for cartilage repair	2699:2747	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	17	91	theme	therapy	2711:2717	arg1	use					2617:2619	the use	2613:2619	the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs	2613:2693	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	6	92	dep	METHODS	857:863	arg1	created					907:913	created	907:913	were created in the trochlear grooves of rat femurs	902:952	METHODS Critical-sized osteochondral defects were created in the trochlear grooves of rat femurs.					
30640523	8	93	theme	%	1256:1256	arg1	hydrogel					1261:1268	4% HA hydrogel	1255:1268	4% HA hydrogel without MSCs	1255:1281	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	1	94	theme	mesenchymal	215:225	arg1	hUCB-MSCs					239:247	hUCB-MSCs	239:247	hUCB-MSCs	239:247	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	94	theme	mesenchymal	215:225	arg1	cells					232:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells	169:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs)	169:248	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	14	95	dep	treatment	2249:2257	arg1	<					2263:2263	P < .05	2261:2267	P < .05	2261:2267	The histological scores also revealed that cartilage repair with undiff-MSCs was better than that with chondro-MSCs, HA alone, or no treatment ( P < .05 in all).					
30640523	3	96	theme	predifferentiation	587:604	arg1	effects					563:569	the effects	559:569	the effects of chondrogenic predifferentiation of hUCB-MSCs on cartilage repair	559:637	Moreover, little study has been conducted on the effects of chondrogenic predifferentiation of hUCB-MSCs on cartilage repair.					
30640523	8	97	from	rats	1393:1396	arg1	defects					1341:1347	the defects	1337:1347	the defects in the left knees	1337:1365	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	8	97	from	rats	1393:1396	arg1	untreated					1377:1385	untreated	1377:1385	untreated	1377:1385	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	17	98	theme	MSCs	2641:2644	arg1	use					2617:2619	the use	2613:2619	the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs	2613:2693	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	17	98	theme	MSCs	2641:2644	arg1	strategy					2719:2726	a stem cell therapy strategy	2699:2726	a stem cell therapy strategy for cartilage repair	2699:2747	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	10	99	theme	no	1591:1592	arg1	treatment					1594:1602	no treatment	1591:1602	no treatment	1591:1602	RESULTS Transplanting undiff-MSCs resulted in overall superior cartilage repair as compared with chondro-MSCs, HA alone, or no treatment.					
30640523	1	100	theme	low	409:411	arg1	immunogenicity					413:426	low immunogenicity	409:426	low immunogenicity	409:426	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	8	101	theme	control	1239:1245	arg1	groups					1247:1252	the control groups	1235:1252	the control groups	1235:1252	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	4	102	theme	mesenchymal	749:759	arg1	MSCs					773:776	MSCs	773:776	MSCs	773:776	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	4	102	theme	mesenchymal	749:759	arg1	cells					766:770	predifferentiated mesenchymal stem cells	731:770	transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs)	680:777	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	7	103	theme	hydrogel	1066:1073	arg1	composite					969:977	a composite	967:977	a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel	967:1073	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	7	103	theme	hydrogel	1066:1073	arg1	hydrogel					1066:1073	4% hyaluronic acid (HA) hydrogel	1042:1073	4% hyaluronic acid (HA) hydrogel	1042:1073	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	7	103	theme	hydrogel	1066:1073	arg1	hUCB-MSCs					1013:1021	chondrogenic predifferentiated hUCB-MSCs	982:1021	chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs)	982:1036	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	3	104	theme	cartilage	622:630	arg1	repair					632:637	cartilage repair	622:637	cartilage repair	622:637	Moreover, little study has been conducted on the effects of chondrogenic predifferentiation of hUCB-MSCs on cartilage repair.					
30640523	17	105	theme	chondrogenic	2659:2670	arg1	MSCs					2690:2693	chondrogenic predifferentiated MSCs	2659:2693	chondrogenic predifferentiated MSCs	2659:2693	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	4	106	theme	cells	766:770	arg1	effectiveness					663:675	the effectiveness	659:675	the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects	659:812	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	13	107	theme	type	2069:2072	arg1	collagen					2077:2084	type II collagen	2069:2084	type II collagen	2069:2084	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	3	108	theme	hUCB-MSCs	609:617	arg1	predifferentiation					587:604	chondrogenic predifferentiation	574:604	chondrogenic predifferentiation of hUCB-MSCs	574:617	Moreover, little study has been conducted on the effects of chondrogenic predifferentiation of hUCB-MSCs on cartilage repair.					
30640523	7	109	theme	hyaluronic	1045:1054	arg1	HA					1062:1063	HA	1062:1063	HA	1062:1063	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	7	109	theme	hyaluronic	1045:1054	arg1	acid					1056:1059	hyaluronic acid	1045:1059	4% hyaluronic acid (HA) hydrogel	1042:1073	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	4	110	theme	undifferentiated	694:709	arg1	MSCs					773:776	MSCs	773:776	MSCs	773:776	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	4	110	theme	undifferentiated	694:709	arg1	cells					766:770	predifferentiated mesenchymal stem cells	731:770	transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs)	680:777	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	0	111	theme	Human	105:109	arg1	Blood					126:130	Human Umbilical Cord Blood	105:130	Human Umbilical Cord Blood	105:130	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	1	112	theme	noninvasive	342:352	arg1	collection					354:363	the noninvasive collection	338:363	the noninvasive collection	338:363	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	7	113	theme	4	1042:1042	arg1	%					1043:1043	%	1043:1043	%	1043:1043	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	15	114	dep	CONCLUSION	2278:2287	arg1	demonstrated					2300:2311	demonstrated	2300:2311	demonstrated that treatment with undiff-MSCs resulted in more favorable cartilage repair than that with chondro-MSCs in a rat model	2300:2430	CONCLUSION This study demonstrated that treatment with undiff-MSCs resulted in more favorable cartilage repair than that with chondro-MSCs in a rat model.					
30640523	0	115	theme	Cartilage	136:144	arg1	Repair					146:151	Cartilage Repair	136:151	Cartilage Repair in a Rat Model	136:166	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	4	116	theme	chondrogenic	718:729	arg1	MSCs					773:776	MSCs	773:776	MSCs	773:776	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	4	116	theme	chondrogenic	718:729	arg1	cells					766:770	predifferentiated mesenchymal stem cells	731:770	transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs)	680:777	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	14	117	theme	P	2261:2261	arg1	<					2263:2263	P < .05	2261:2267	P < .05	2261:2267	The histological scores also revealed that cartilage repair with undiff-MSCs was better than that with chondro-MSCs, HA alone, or no treatment ( P < .05 in all).					
30640523	0	118	theme	Cord	121:124	arg1	Blood					126:130	Human Umbilical Cord Blood	105:130	Human Umbilical Cord Blood	105:130	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	8	119	from	defects	1305:1311	arg1	knees					1326:1330	the right knees	1316:1330	the right knees	1316:1330	In the control groups, 4% HA hydrogel without MSCs was transplanted into defects in the right knees, and the defects in the left knees were left untreated in 20 rats.					
30640523	4	120	theme	osteochondral	792:804	arg1	defects					806:812	osteochondral defects	792:812	osteochondral defects	792:812	PURPOSE To compare the effectiveness of transplanting undifferentiated versus chondrogenic predifferentiated mesenchymal stem cells (MSCs) for treating osteochondral defects.					
30640523	13	121	theme	articular	2012:2020	arg1	tissue					2032:2037	surrounding normal articular cartilage tissue	1993:2037	surrounding normal articular cartilage tissue	1993:2037	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	2	122	dep	in	442:443	arg1	vivo					445:448	vivo	445:448	vivo	445:448	However, few in vivo studies have reported the use of hUCB-MSCs on cartilage repair.					
30640523	2	123	from	use	476:478	arg1	repair					506:511	cartilage repair	496:511	cartilage repair	496:511	However, few in vivo studies have reported the use of hUCB-MSCs on cartilage repair.					
30640523	1	124	link	blood-derived	201:213	arg1	hUCB-MSCs					239:247	hUCB-MSCs	239:247	hUCB-MSCs	239:247	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	124	link	blood-derived	201:213	arg1	cells					232:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells	169:236	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs)	169:248	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	17	125	dep	RELEVANCE	2569:2577	arg1	support					2605:2611	support	2605:2611	support	2605:2611	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	1	126	theme	expansion	385:393	arg1	capacity					395:402	high expansion capacity	380:402	high expansion capacity	380:402	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	7	127	theme	predifferentiated	995:1011	arg1	chondro-MSCs					1024:1035	chondro-MSCs	1024:1035	chondro-MSCs	1024:1035	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	7	127	theme	predifferentiated	995:1011	arg1	hUCB-MSCs					1013:1021	chondrogenic predifferentiated hUCB-MSCs	982:1021	chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs)	982:1036	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	15	128	theme	favorable	2362:2370	arg1	repair					2382:2387	more favorable cartilage repair	2357:2387	more favorable cartilage repair	2357:2387	CONCLUSION This study demonstrated that treatment with undiff-MSCs resulted in more favorable cartilage repair than that with chondro-MSCs in a rat model.					
30640523	1	129	theme	cell	291:294	arg1	source					296:301	a promising cell source	279:301	a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity	279:426	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	1	129	theme	cell	291:294	arg1	interest					267:274	much interest	262:274	much interest	262:274	BACKGROUND Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have gained much interest as a promising cell source for regenerative medicine owing to the noninvasive collection, availability, high expansion capacity, and low immunogenicity.					
30640523	2	130	theme	few	438:440	arg1	studies					450:456	few in vivo studies	438:456	few in vivo studies	438:456	However, few in vivo studies have reported the use of hUCB-MSCs on cartilage repair.					
30640523	7	131	theme	%	1179:1179	arg1	hydrogel					1184:1191	4% HA hydrogel	1178:1191	4% HA hydrogel	1178:1191	In 20 rats, a composite of chondrogenic predifferentiated hUCB-MSCs (chondro-MSCs) and 4% hyaluronic acid (HA) hydrogel was transplanted into defects in the right knees, whereas undifferentiated hUCB-MSCs (undiff-MSCs) and 4% HA hydrogel were transplanted into the left knees.					
30640523	5	132	theme	laboratory	839:848	arg1	study					850:854	laboratory study	839:854	laboratory study	839:854	STUDY DESIGN Controlled laboratory study.					
30640523	12	133	theme	articular	1827:1835	arg1	tissue					1847:1852	surrounding normal articular cartilage tissue	1808:1852	surrounding normal articular cartilage tissue	1808:1852	The undiff-MSC group showed cellular morphology and arrangement similar to surrounding normal articular cartilage tissue at 16 weeks, both of which were also better than those of the other groups.					
30640523	13	134	theme	cartilage	2022:2030	arg1	tissue					2032:2037	surrounding normal articular cartilage tissue	1993:2037	surrounding normal articular cartilage tissue	1993:2037	In addition, the undiff-MSC group showed coloration similar to surrounding normal articular cartilage tissue at 16 weeks in safranin O and type II collagen immunohistochemical staining.					
30640523	17	135	theme	cartilage	2732:2740	arg1	repair					2742:2747	cartilage repair	2732:2747	cartilage repair	2732:2747	CLINICAL RELEVANCE The results of this study support the use of undifferentiated MSCs, rather than chondrogenic predifferentiated MSCs, as a stem cell therapy strategy for cartilage repair.					
30640523	16	136	theme	MSCs	2497:2500	arg1	predifferentiation					2475:2492	chondrogenic predifferentiation	2462:2492	chondrogenic predifferentiation of MSCs before transplantation	2462:2523	These findings indicate that chondrogenic predifferentiation of MSCs before transplantation does not enhance cartilage repair.					
30640523	0	137	from	Repair	146:151	arg1	Model					162:166	a Rat Model	156:166	a Rat Model	156:166	Comparison of Undifferentiated Versus Chondrogenic Predifferentiated Mesenchymal Stem Cells Derived From Human Umbilical Cord Blood for Cartilage Repair in a Rat Model.					
30640523	10	138	theme	cartilage	1530:1538	arg1	repair					1540:1545	overall superior cartilage repair	1513:1545	overall superior cartilage repair	1513:1545	RESULTS Transplanting undiff-MSCs resulted in overall superior cartilage repair as compared with chondro-MSCs, HA alone, or no treatment.					
30770656	3	0	theme	cartilage	751:759	arg1	constructs					761:770	self-assembled articular cartilage constructs	726:770	self-assembled articular cartilage constructs	726:770	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	5	1	theme	combined	1562:1569	arg1	treatments					1571:1580	combined treatments	1562:1580	combined treatments	1562:1580	Compressive relaxation modulus was increased 5.9-fold with addition of LOXL2 and 3.4-fold with combined treatments over CTL.					
30770656	3	2	theme	lysyl	558:562	arg1	LOXL2					588:592	LOXL2	588:592	LOXL2	588:592	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	2	theme	lysyl	558:562	arg1	protein-2					577:585	exogenous lysyl oxidase-like protein-2	548:585	exogenous lysyl oxidase-like protein-2 (LOXL2)	548:593	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	1	3	theme	tissue	286:291	arg1	equivalents					293:303	engineered tissue equivalents	275:303	engineered tissue equivalents	275:303	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
30770656	5	4	theme	LOXL2	1538:1542	arg1	addition					1526:1533	addition	1526:1533	addition of LOXL2	1526:1542	Compressive relaxation modulus was increased 5.9-fold with addition of LOXL2 and 3.4-fold with combined treatments over CTL.					
30770656	6	5	theme	tensile	1653:1659	arg1	R2					1673:1674	R2	1673:1674	R2 = 0.82; p < 0.001	1673:1692	FLIm parameters had strong and significant correlations with tensile properties (R2 = 0.82; p < 0.001) and compressive properties (R2 = 0.59; p < 0.001).					
30770656	6	5	theme	tensile	1653:1659	arg1	properties					1661:1670	tensile properties	1653:1670	tensile properties (R2 = 0.82; p < 0.001)	1653:1693	FLIm parameters had strong and significant correlations with tensile properties (R2 = 0.82; p < 0.001) and compressive properties (R2 = 0.59; p < 0.001).					
30770656	3	6	theme	correlations	1084:1095	arg1	sufficient					1100:1109	sufficient	1100:1109	sufficient	1100:1109	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	6	theme	correlations	1084:1095	arg1	strength					1066:1073	the strength	1062:1073	the strength of these correlations	1062:1095	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	4	7	theme	tensile	1418:1424	arg1	strength					1426:1433	tensile strength	1418:1433	tensile strength	1418:1433	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	8	8	theme	FLIm-based	1994:2003	arg1	tools					2005:2009	FLIm-based tools	1994:2009	FLIm-based tools	1994:2009	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	3	9	theme	testing	1134:1140	arg1	methods					1142:1148	destructive testing methods	1122:1148	destructive testing methods	1122:1148	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	10	theme	proteoglycan	627:638	arg1	LINK					656:659	LINK	656:659	LINK	656:659	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	10	theme	proteoglycan	627:638	arg1	protein-1					645:653	proteoglycan link protein-1	627:653	proteoglycan link protein-1 (LINK)	627:660	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	11	theme	label-free	794:803	arg1	imaging					844:850	label-free, non-destructive fluorescence lifetime imaging	794:850	label-free, non-destructive fluorescence lifetime imaging (FLIm)	794:857	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	11	theme	label-free	794:803	arg1	FLIm					853:856	FLIm	853:856	FLIm	853:856	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	12	theme	lifetime	835:842	arg1	imaging					844:850	label-free, non-destructive fluorescence lifetime imaging	794:850	label-free, non-destructive fluorescence lifetime imaging (FLIm)	794:857	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	12	theme	lifetime	835:842	arg1	FLIm					853:856	FLIm	853:856	FLIm	853:856	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	13	dep	form	956:959	arg1	c					953:953	c	953:953	c	953:953	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	8	14	theme	tissue-engineered	2070:2086	arg1	cartilage					2088:2096	tissue-engineered cartilage	2070:2096	tissue-engineered cartilage	2070:2096	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	1	15	theme	expensive	161:169	arg1	techniques					204:213	expensive, time-consuming, and destructive techniques	161:213	expensive, time-consuming, and destructive techniques	161:213	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
30770656	7	16	theme	maturation	1825:1834	arg1	assessment					1836:1845	tissue maturation assessment	1818:1845	tissue maturation assessment	1818:1845	SVM learning based on FLIm-derived parameters was capable of automating tissue maturation assessment with a discriminant ability of 98.4%.					
30770656	0	17	theme	mechanical	75:84	arg1	properties					86:95	enhanced mechanical properties	66:95	enhanced mechanical properties of self-assembled articular cartilage	66:133	Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.					
30770656	3	18	dep	label-free	794:803	arg1	non-destructive					806:820	non-destructive	806:820	non-destructive	806:820	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	1	19	theme	time-consuming	172:185	arg1	techniques					204:213	expensive, time-consuming, and destructive techniques	161:213	expensive, time-consuming, and destructive techniques	161:213	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
30770656	8	20	theme	marked	1906:1911	arg1	improvements					1913:1924	marked improvements	1906:1924	marked improvements in mechanical properties with matrix stabilization	1906:1975	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	3	21	theme	biomechanical	926:938	arg1	properties					940:949	biomechanical properties	926:949	biomechanical properties	926:949	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	4	22	theme	proteins	1308:1315	arg1	combination					1268:1278	The combination	1264:1278	The combination of exogenous LOXL2 and LINK proteins	1264:1315	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	0	23	theme	articular	115:123	arg1	cartilage					125:133	self-assembled articular cartilage	100:133	self-assembled articular cartilage	100:133	Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.					
30770656	2	24	theme	products	453:460	arg1	development					420:430	the development	416:430	the development of tissue-engineered products	416:460	A non-destructive solution to monitor tissue quality and maturation would greatly reduce costs and accelerate the development of tissue-engineered products.					
30770656	6	25	contain	had	1608:1610	arg1	parameters					1597:1606	FLIm parameters	1592:1606	FLIm parameters	1592:1606	FLIm parameters had strong and significant correlations with tensile properties (R2 = 0.82; p < 0.001) and compressive properties (R2 = 0.59; p < 0.001).					
30770656	6	25	contain	had	1608:1610	arg2	correlations					1635:1646	strong and significant correlations	1612:1646	strong and significant correlations	1612:1646	FLIm parameters had strong and significant correlations with tensile properties (R2 = 0.82; p < 0.001) and compressive properties (R2 = 0.59; p < 0.001).					
30770656	4	26	from	increase	1406:1413	arg1	density					1382:1388	collagen crosslinking density	1360:1388	collagen crosslinking density	1360:1388	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	4	26	from	increase	1406:1413	arg1	strength					1426:1433	tensile strength	1418:1433	tensile strength	1418:1433	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	4	27	theme	exogenous	1283:1291	arg1	LOXL2					1293:1297	exogenous LOXL2 and LINK proteins	1283:1315	LOXL2	1293:1297	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	0	28	theme	Non-destructive	0:14	arg1	detection					16:24	Non-destructive detection	0:24	Non-destructive detection of matrix stabilization	0:48	Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.					
30770656	5	29	with	5.9-fold	1512:1519	arg1	addition					1526:1533	addition	1526:1533	addition of LOXL2	1526:1542	Compressive relaxation modulus was increased 5.9-fold with addition of LOXL2 and 3.4-fold with combined treatments over CTL.					
30770656	8	30	theme	matrix	1956:1961	arg1	stabilization					1963:1975	matrix stabilization	1956:1975	matrix stabilization	1956:1975	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	3	31	theme	destructive	996:1006	arg1	measurements					1028:1039	destructive and non-destructive measurements	996:1039	destructive and non-destructive measurements	996:1039	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	4	32	theme	crosslinking	1369:1380	arg1	density					1382:1388	collagen crosslinking density	1360:1388	collagen crosslinking density	1360:1388	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	1	33	theme	Tissue	136:141	arg1	engineers					143:151	Tissue engineers	136:151	Tissue engineers	136:151	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
30770656	3	34	theme	tensile	704:710	arg1	properties					712:721	increased compressive and tensile properties	678:721	increased compressive and tensile properties	678:721	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	0	35	theme	stabilization	36:48	arg1	detection					16:24	Non-destructive detection	0:24	Non-destructive detection of matrix stabilization	0:48	Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.					
30770656	3	36	theme	vector	1185:1190	arg1	machine					1192:1198	support vector machine	1177:1198	support vector machine (SVM) learning	1177:1213	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	36	theme	vector	1185:1190	arg1	SVM					1201:1203	SVM	1201:1203	SVM	1201:1203	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	37	theme	compressive	688:698	arg1	properties					712:721	increased compressive and tensile properties	678:721	increased compressive and tensile properties	678:721	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	5	38	theme	Compressive	1467:1477	arg1	modulus					1490:1496	Compressive relaxation modulus	1467:1496	Compressive relaxation modulus	1467:1496	Compressive relaxation modulus was increased 5.9-fold with addition of LOXL2 and 3.4-fold with combined treatments over CTL.					
30770656	3	39	dep	evaluate	777:784	arg1	b					774:774	b	774:774	b	774:774	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	40	theme	non-destructive	1012:1026	arg1	measurements					1028:1039	destructive and non-destructive measurements	996:1039	destructive and non-destructive measurements	996:1039	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	4	41	from	increase	1348:1355	arg1	density					1382:1388	collagen crosslinking density	1360:1388	collagen crosslinking density	1360:1388	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	4	41	from	increase	1348:1355	arg1	strength					1426:1433	tensile strength	1418:1433	tensile strength	1418:1433	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	4	42	theme	4.9-fold	1339:1346	arg1	increase					1348:1355	a synergistic 4.9-fold increase	1325:1355	a synergistic 4.9-fold increase in collagen crosslinking density	1325:1388	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	3	43	from	properties	712:721	arg1	constructs					761:770	self-assembled articular cartilage constructs	726:770	self-assembled articular cartilage constructs	726:770	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	44	theme	oxidase-like	564:575	arg1	LOXL2					588:592	LOXL2	588:592	LOXL2	588:592	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	44	theme	oxidase-like	564:575	arg1	protein-2					577:585	exogenous lysyl oxidase-like protein-2	548:585	exogenous lysyl oxidase-like protein-2 (LOXL2)	548:593	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	0	45	theme	enhanced	66:73	arg1	properties					86:95	enhanced mechanical properties	66:95	enhanced mechanical properties of self-assembled articular cartilage	66:133	Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.					
30770656	3	46	dep	determine	504:512	arg1	a					501:501	a	501:501	a	501:501	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	47	theme	exogenous	548:556	arg1	LOXL2					588:592	LOXL2	588:592	LOXL2	588:592	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	47	theme	exogenous	548:556	arg1	protein-2					577:585	exogenous lysyl oxidase-like protein-2	548:585	exogenous lysyl oxidase-like protein-2 (LOXL2)	548:593	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	48	dep	replace	1114:1120	arg1	determine					1159:1167	determine	1159:1167	determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking	1159:1261	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	1	49	theme	engineered	275:284	arg1	equivalents					293:303	engineered tissue equivalents	275:303	engineered tissue equivalents	275:303	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
30770656	3	50	theme	LOXL2-induced	1227:1239	arg1	crosslinking					1250:1261	LOXL2-induced collagen crosslinking	1227:1261	LOXL2-induced collagen crosslinking	1227:1261	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	8	51	theme	great	2016:2020	arg1	potential					2022:2030	great potential	2016:2030	great potential	2016:2030	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	1	52	theme	equivalents	293:303	arg1	function					263:270	function	263:270	function	263:270	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
30770656	1	52	theme	equivalents	293:303	arg1	composition					230:240	composition	230:240	composition	230:240	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
30770656	1	52	theme	equivalents	293:303	arg1	microstructure					243:256	microstructure	243:256	microstructure	243:256	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
30770656	7	53	theme	%	1882:1882	arg1	ability					1867:1873	a discriminant ability	1852:1873	a discriminant ability of 98.4%	1852:1882	SVM learning based on FLIm-derived parameters was capable of automating tissue maturation assessment with a discriminant ability of 98.4%.					
30770656	8	54	with	properties	1940:1949	arg1	stabilization					1963:1975	matrix stabilization	1956:1975	matrix stabilization	1956:1975	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	4	55	theme	8.3-fold	1397:1404	arg1	increase					1406:1413	an 8.3-fold increase	1394:1413	an 8.3-fold increase in tensile strength	1394:1433	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	2	56	theme	non-destructive	308:322	arg1	solution					324:331	A non-destructive solution	306:331	A non-destructive solution to monitor tissue quality and maturation	306:372	A non-destructive solution to monitor tissue quality and maturation would greatly reduce costs and accelerate the development of tissue-engineered products.					
30770656	1	57	theme	destructive	192:202	arg1	techniques					204:213	expensive, time-consuming, and destructive techniques	161:213	expensive, time-consuming, and destructive techniques	161:213	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
30770656	3	58	theme	destructive	1122:1132	arg1	methods					1142:1148	destructive testing methods	1122:1148	destructive testing methods	1122:1148	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	8	59	contain	have	2011:2014	arg1	tools					2005:2009	FLIm-based tools	1994:2009	FLIm-based tools	1994:2009	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	8	59	contain	have	2011:2014	arg2	potential					2022:2030	great potential	2016:2030	great potential	2016:2030	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	3	60	with	stabilization	529:541	arg1	hyaluronan					612:621	recombinant hyaluronan	600:621	recombinant hyaluronan	600:621	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	60	with	stabilization	529:541	arg1	LINK					656:659	LINK	656:659	LINK	656:659	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	60	with	stabilization	529:541	arg1	LOXL2					588:592	LOXL2	588:592	LOXL2	588:592	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	60	with	stabilization	529:541	arg1	protein-1					645:653	proteoglycan link protein-1	627:653	proteoglycan link protein-1 (LINK)	627:660	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	60	with	stabilization	529:541	arg1	protein-2					577:585	exogenous lysyl oxidase-like protein-2	548:585	exogenous lysyl oxidase-like protein-2 (LOXL2)	548:593	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	6	61	theme	significant	1623:1633	arg1	correlations					1635:1646	strong and significant correlations	1612:1646	strong and significant correlations	1612:1646	FLIm parameters had strong and significant correlations with tensile properties (R2 = 0.82; p < 0.001) and compressive properties (R2 = 0.59; p < 0.001).					
30770656	7	62	theme	discriminant	1854:1865	arg1	ability					1867:1873	a discriminant ability	1852:1873	a discriminant ability of 98.4%	1852:1882	SVM learning based on FLIm-derived parameters was capable of automating tissue maturation assessment with a discriminant ability of 98.4%.					
30770656	3	63	theme	fluorescence	822:833	arg1	imaging					844:850	label-free, non-destructive fluorescence lifetime imaging	794:850	label-free, non-destructive fluorescence lifetime imaging (FLIm)	794:857	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	63	theme	fluorescence	822:833	arg1	FLIm					853:856	FLIm	853:856	FLIm	853:856	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	2	64	theme	tissue	344:349	arg1	quality					351:357	tissue quality	344:357	tissue quality	344:357	A non-destructive solution to monitor tissue quality and maturation would greatly reduce costs and accelerate the development of tissue-engineered products.					
30770656	6	65	theme	strong	1612:1617	arg1	correlations					1635:1646	strong and significant correlations	1612:1646	strong and significant correlations	1612:1646	FLIm parameters had strong and significant correlations with tensile properties (R2 = 0.82; p < 0.001) and compressive properties (R2 = 0.59; p < 0.001).					
30770656	8	66	theme	non-destructive	2040:2054	arg1	assessment					2056:2065	the non-destructive assessment	2036:2065	the non-destructive assessment of tissue-engineered cartilage	2036:2096	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	3	67	theme	link	640:643	arg1	LINK					656:659	LINK	656:659	LINK	656:659	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	67	theme	link	640:643	arg1	protein-1					645:653	proteoglycan link protein-1	627:653	proteoglycan link protein-1 (LINK)	627:660	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	68	theme	articular	741:749	arg1	constructs					761:770	self-assembled articular cartilage constructs	726:770	self-assembled articular cartilage constructs	726:770	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	8	69	from	improvements	1913:1924	arg1	properties					1940:1949	mechanical properties	1929:1949	mechanical properties with matrix stabilization	1929:1975	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	8	70	theme	cartilage	2088:2096	arg1	assessment					2056:2065	the non-destructive assessment	2036:2065	the non-destructive assessment of tissue-engineered cartilage	2036:2096	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	3	71	dep	infer	876:880	arg1	form					956:959	form	956:959	(c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking	952:1261	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	72	theme	recombinant	600:610	arg1	hyaluronan					612:621	recombinant hyaluronan	600:621	recombinant hyaluronan	600:621	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	73	theme	biochemical	898:908	arg1	composition					910:920	biochemical composition	898:920	biochemical composition	898:920	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	0	74	theme	self-assembled	100:113	arg1	cartilage					125:133	self-assembled articular cartilage	100:133	self-assembled articular cartilage	100:133	Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.					
30770656	7	75	theme	tissue	1818:1823	arg1	assessment					1836:1845	tissue maturation assessment	1818:1845	tissue maturation assessment	1818:1845	SVM learning based on FLIm-derived parameters was capable of automating tissue maturation assessment with a discriminant ability of 98.4%.					
30770656	3	76	used	used	868:871	arg2	FLIm					853:856	FLIm	853:856	FLIm	853:856	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	76	used	used	868:871	arg2	imaging					844:850	label-free, non-destructive fluorescence lifetime imaging	794:850	label-free, non-destructive fluorescence lifetime imaging (FLIm)	794:857	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	0	77	theme	cartilage	125:133	arg1	properties					86:95	enhanced mechanical properties	66:95	enhanced mechanical properties of self-assembled articular cartilage	66:133	Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.					
30770656	4	78	theme	synergistic	1327:1337	arg1	increase					1348:1355	a synergistic 4.9-fold increase	1325:1355	a synergistic 4.9-fold increase in collagen crosslinking density	1325:1388	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	7	79	link	FLIm-derived	1768:1779	arg1	parameters					1781:1790	FLIm-derived parameters	1768:1790	FLIm-derived parameters	1768:1790	SVM learning based on FLIm-derived parameters was capable of automating tissue maturation assessment with a discriminant ability of 98.4%.					
30770656	3	80	theme	quantitative	961:972	arg1	relationships					974:986	quantitative relationships	961:986	quantitative relationships between destructive and non-destructive measurements	961:1039	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	4	81	theme	LOXL2	1293:1297	arg1	combination					1268:1278	The combination	1264:1278	The combination of exogenous LOXL2 and LINK proteins	1264:1315	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	2	82	theme	tissue-engineered	435:451	arg1	products					453:460	tissue-engineered products	435:460	tissue-engineered products	435:460	A non-destructive solution to monitor tissue quality and maturation would greatly reduce costs and accelerate the development of tissue-engineered products.					
30770656	8	83	theme	mechanical	1929:1938	arg1	properties					1940:1949	mechanical properties	1929:1949	mechanical properties with matrix stabilization	1929:1975	These results showed marked improvements in mechanical properties with matrix stabilization and suggest that FLIm-based tools have great potential for the non-destructive assessment of tissue-engineered cartilage.					
30770656	7	84	theme	FLIm-derived	1768:1779	arg1	parameters					1781:1790	FLIm-derived parameters	1768:1790	FLIm-derived parameters	1768:1790	SVM learning based on FLIm-derived parameters was capable of automating tissue maturation assessment with a discriminant ability of 98.4%.					
30770656	4	85	theme	LINK	1303:1306	arg1	proteins					1308:1315	exogenous LOXL2 and LINK proteins	1283:1315	proteins	1308:1315	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	3	86	theme	study	486:490	arg1	objectives					467:476	The objectives	463:476	The objectives of this study	463:490	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	0	87	theme	matrix	29:34	arg1	stabilization					36:48	matrix stabilization	29:48	matrix stabilization	29:48	Non-destructive detection of matrix stabilization correlates with enhanced mechanical properties of self-assembled articular cartilage.					
30770656	3	88	theme	machine	1192:1198	arg1	learning					1206:1213	support vector machine (SVM) learning	1177:1213	support vector machine (SVM) learning	1177:1213	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	6	89	theme	compressive	1699:1709	arg1	R2					1723:1724	R2	1723:1724	R2 = 0.59; p < 0.001	1723:1742	FLIm parameters had strong and significant correlations with tensile properties (R2 = 0.82; p < 0.001) and compressive properties (R2 = 0.59; p < 0.001).					
30770656	6	89	theme	compressive	1699:1709	arg1	properties					1711:1720	compressive properties	1699:1720	compressive properties (R2 = 0.59; p < 0.001)	1699:1743	FLIm parameters had strong and significant correlations with tensile properties (R2 = 0.82; p < 0.001) and compressive properties (R2 = 0.59; p < 0.001).					
30770656	3	90	theme	support	1177:1183	arg1	machine					1192:1198	support vector machine	1177:1198	support vector machine (SVM) learning	1177:1213	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	90	theme	support	1177:1183	arg1	SVM					1201:1203	SVM	1201:1203	SVM	1201:1203	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	7	91	theme	SVM	1746:1748	arg1	learning					1750:1757	SVM learning	1746:1757	SVM learning based on FLIm-derived parameters	1746:1790	SVM learning based on FLIm-derived parameters was capable of automating tissue maturation assessment with a discriminant ability of 98.4%.					
30770656	3	92	theme	increased	678:686	arg1	properties					712:721	increased compressive and tensile properties	678:721	increased compressive and tensile properties	678:721	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	93	dep	determine	1159:1167	arg1	d					1156:1156	d	1156:1156	d	1156:1156	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	94	theme	self-assembled	726:739	arg1	constructs					761:770	self-assembled articular cartilage constructs	726:770	self-assembled articular cartilage constructs	726:770	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	5	95	theme	relaxation	1479:1488	arg1	modulus					1490:1496	Compressive relaxation modulus	1467:1496	Compressive relaxation modulus	1467:1496	Compressive relaxation modulus was increased 5.9-fold with addition of LOXL2 and 3.4-fold with combined treatments over CTL.					
30770656	6	96	theme	FLIm	1592:1595	arg1	parameters					1597:1606	FLIm parameters	1592:1606	FLIm parameters	1592:1606	FLIm parameters had strong and significant correlations with tensile properties (R2 = 0.82; p < 0.001) and compressive properties (R2 = 0.59; p < 0.001).					
30770656	4	97	theme	collagen	1360:1367	arg1	density					1382:1388	collagen crosslinking density	1360:1388	collagen crosslinking density	1360:1388	The combination of exogenous LOXL2 and LINK proteins created a synergistic 4.9-fold increase in collagen crosslinking density and an 8.3-fold increase in tensile strength as compared with control (CTL).					
30770656	3	98	theme	matrix	522:527	arg1	stabilization					529:541	matrix stabilization	522:541	matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK)	522:660	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	3	99	theme	collagen	1241:1248	arg1	crosslinking					1250:1261	LOXL2-induced collagen crosslinking	1227:1261	LOXL2-induced collagen crosslinking	1227:1261	The objectives of this study were to (a) determine whether matrix stabilization with exogenous lysyl oxidase-like protein-2 (LOXL2) with recombinant hyaluronan and proteoglycan link protein-1 (LINK) would result in increased compressive and tensile properties in self-assembled articular cartilage constructs, (b) evaluate whether label-free, non-destructive fluorescence lifetime imaging (FLIm) could be used to infer changes in both biochemical composition and biomechanical properties, (c) form quantitative relationships between destructive and non-destructive measurements to determine whether the strength of these correlations is sufficient to replace destructive testing methods, and (d) determine whether support vector machine (SVM) learning can predict LOXL2-induced collagen crosslinking.					
30770656	1	100	dep	composition	230:240	arg1	the					226:228	the	226:228	the	226:228	Tissue engineers rely on expensive, time-consuming, and destructive techniques to monitor the composition, microstructure, and function of engineered tissue equivalents.					
31655887	4	0	theme	larger	819:824	arg1	layer					808:812	the HA layer	801:812	the HA layer	801:812	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	4	0	theme	larger	819:824	arg1	amount					850:855	the larger the loaded BMP-2 and CS amount	815:855	the larger the loaded BMP-2 and CS amount	815:855	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	3	1	theme	antibacterial	664:676	arg1	activity					678:685	antibacterial activity	664:685	antibacterial activity	664:685	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	1	2	theme	micro-arc	259:267	arg1	MAO					280:282	MAO	280:282	MAO	280:282	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	1	2	theme	micro-arc	259:267	arg1	oxidation					269:277	one-step micro-arc oxidation	250:277	one-step micro-arc oxidation (MAO)	250:283	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	4	3	theme	loaded	830:835	arg1	BMP-2					837:841	the loaded BMP-2	826:841	the loaded BMP-2	826:841	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	1	4	with	Ti	458:459	arg1	properties					500:509	good antibacterial and biological properties	466:509	good antibacterial and biological properties	466:509	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	5	5	theme	BMP-2	1136:1140	arg1	time					1150:1153	BMP-2 release time	1136:1153	BMP-2 release time	1136:1153	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	4	6	theme	HA	805:806	arg1	layer					808:812	the HA layer	801:812	the HA layer	801:812	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	4	6	theme	HA	805:806	arg1	amount					850:855	the larger the loaded BMP-2 and CS amount	815:855	the larger the loaded BMP-2 and CS amount	815:855	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	3	7	theme	obtained	694:701	arg1	coatings					722:729	the obtained (BMP-2)/CS/HA/TiO2 coatings	690:729	the obtained (BMP-2)/CS/HA/TiO2 coatings	690:729	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	5	8	theme	degradation	1115:1125	arg1	time					1127:1130	the prolonged CS degradation time	1098:1130	the prolonged CS degradation time	1098:1130	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	1	9	theme	dip-coating	430:440	arg1	method					442:447	dip-coating method	430:447	dip-coating method to endow Ti with good antibacterial and biological properties	430:509	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	8	10	theme	layers	1616:1621	arg1	concentrations					1627:1640	concentrations	1627:1640	concentrations of CS and BMP-2 solutions	1627:1666	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	8	10	theme	layers	1616:1621	arg1	thickness					1600:1608	the thickness	1596:1608	the thickness of HA layers	1596:1621	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	7	11	from	BMP-2	1369:1373	arg1	CS					1378:1379	CS	1378:1379	CS	1378:1379	After loading BMP-2 in CS, the BMP-2 can significantly improve cell adhesion, spreading and proliferation, and the loaded amount can also be controlled by the concentration of BMP-2 solution.					
31655887	3	12	theme	/CS/HA/TiO2	710:720	arg1	coatings					722:729	the obtained (BMP-2)/CS/HA/TiO2 coatings	690:729	the obtained (BMP-2)/CS/HA/TiO2 coatings	690:729	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	8	13	theme	CS	1645:1646	arg1	solutions					1658:1666	CS and BMP-2 solutions	1645:1666	CS and BMP-2 solutions	1645:1666	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	7	14	theme	solution	1537:1544	arg1	concentration					1514:1526	the concentration	1510:1526	the concentration of BMP-2 solution	1510:1544	After loading BMP-2 in CS, the BMP-2 can significantly improve cell adhesion, spreading and proliferation, and the loaded amount can also be controlled by the concentration of BMP-2 solution.					
31655887	4	15	theme	better	871:876	arg1	strength					886:893	better bonding strength	871:893	better bonding strength between coatings	871:910	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	1	16	theme	CS	366:367	arg1	coatings					369:376	-encapsulated CS coatings	352:376	-encapsulated CS coatings	352:376	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	8	17	theme	BMP-2	1652:1656	arg1	solutions					1658:1666	CS and BMP-2 solutions	1645:1666	CS and BMP-2 solutions	1645:1666	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	6	18	theme	CS/HA/TiO2	1221:1230	arg1	coatings					1232:1239	All CS/HA/TiO2 coatings	1217:1239	All CS/HA/TiO2 coatings	1217:1239	All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF).					
31655887	5	19	theme	concentration	995:1007	arg1	increase					980:987	the increase	976:987	the increase of CS concentration	976:1007	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	8	20	theme	Ti	1673:1674	arg1	material					1684:1691	the Ti implant material	1669:1691	the Ti implant material with excellent biological and antibacterial properties	1669:1746	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	1	21	theme	hydroxyapatite	156:169	arg1	coatings					191:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	4	22	theme	antibacterial	913:925	arg1	activity					927:934	antibacterial activity	913:934	antibacterial activity	913:934	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	6	23	from	formation	1313:1321	arg1	SBF					1349:1351	SBF	1349:1351	SBF	1349:1351	All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF).					
31655887	6	23	from	formation	1313:1321	arg1	fluids					1341:1346	simulated body fluids	1326:1346	simulated body fluids (SBF)	1326:1352	All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF).					
31655887	1	24	theme	HA	172:173	arg1	coatings					191:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	5	25	theme	improved	1169:1176	arg1	antibacterial					1178:1190	improved antibacterial	1169:1190	improved antibacterial	1169:1190	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	0	26	theme	bone	107:110	arg1	engineering					119:129	bone tissue engineering	107:129	bone tissue engineering	107:129	Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.					
31655887	8	27	theme	excellent	1698:1706	arg1	properties					1737:1746	excellent biological and antibacterial properties	1698:1746	excellent biological and antibacterial properties	1698:1746	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	1	28	theme	/TiO2	175:179	arg1	coatings					191:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	0	29	from	Preparation	0:10	arg1	surfaces					94:101	titanium surfaces	85:101	titanium surfaces for bone tissue engineering	85:129	Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.					
31655887	1	30	theme	morphogenic	323:333	arg1	protein-2					335:343	bone morphogenic protein-2	318:343	bone morphogenic protein-2 (BMP-2)	318:351	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	1	30	theme	morphogenic	323:333	arg1	BMP-2					346:350	BMP-2	346:350	BMP-2	346:350	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	0	31	theme	BMP-2/chitosan/hydroxyapatite	15:43	arg1	coatings					73:80	BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings	15:80	BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings	15:80	Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.					
31655887	5	32	theme	prolonged	1102:1110	arg1	time					1127:1130	the prolonged CS degradation time	1098:1130	the prolonged CS degradation time	1098:1130	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	0	33	theme	bio-composite	59:71	arg1	coatings					73:80	BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings	15:80	BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings	15:80	Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.					
31655887	3	34	dep	obtained	694:701	arg1	BMP-2					704:708	BMP-2	704:708	BMP-2	704:708	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	3	35	theme	release	612:618	arg1	behavior					620:627	BMP-2 release behavior	606:627	BMP-2 release behavior	606:627	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	4	36	theme	thicker	793:799	arg1	layer					808:812	the HA layer	801:812	the HA layer	801:812	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	4	36	theme	thicker	793:799	arg1	amount					850:855	the larger the loaded BMP-2 and CS amount	815:855	the larger the loaded BMP-2 and CS amount	815:855	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	6	37	theme	body	1336:1339	arg1	SBF					1349:1351	SBF	1349:1351	SBF	1349:1351	All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF).					
31655887	6	37	theme	body	1336:1339	arg1	fluids					1341:1346	simulated body fluids	1326:1346	simulated body fluids (SBF)	1326:1352	All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF).					
31655887	1	38	theme	antibacterial	471:483	arg1	properties					500:509	good antibacterial and biological properties	466:509	good antibacterial and biological properties	466:509	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	8	39	theme	present	1551:1557	arg1	study					1559:1563	The present study	1547:1563	The present study	1547:1563	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	5	40	theme	release	1142:1148	arg1	time					1150:1153	BMP-2 release time	1136:1153	BMP-2 release time	1136:1153	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	1	41	theme	biological	489:498	arg1	properties					500:509	good antibacterial and biological properties	466:509	good antibacterial and biological properties	466:509	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	5	42	theme	CS	1077:1078	arg1	increase					1065:1072	the increase	1061:1072	the increase of CS	1061:1078	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	1	43	theme	one-step	250:257	arg1	MAO					280:282	MAO	280:282	MAO	280:282	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	1	43	theme	one-step	250:257	arg1	oxidation					269:277	one-step micro-arc oxidation	250:277	one-step micro-arc oxidation (MAO)	250:283	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	1	44	theme	HA/TiO2	410:416	arg1	surfaces					418:425	the HA/TiO2 surfaces	406:425	the HA/TiO2 surfaces	406:425	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	2	45	theme	scratch	565:571	arg1	method					573:578	scratch method	565:578	scratch method	565:578	The bonding strength between coatings was studied by scratch method.					
31655887	7	46	theme	loading	1361:1367	arg1	BMP-2					1369:1373	loading BMP-2	1361:1373	loading BMP-2 in CS	1361:1379	After loading BMP-2 in CS, the BMP-2 can significantly improve cell adhesion, spreading and proliferation, and the loaded amount can also be controlled by the concentration of BMP-2 solution.					
31655887	1	47	theme	composite	181:189	arg1	coatings					191:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	6	48	theme	HA	1310:1311	arg1	formation					1313:1321	HA formation	1310:1321	HA formation in simulated body fluids (SBF)	1310:1352	All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF).					
31655887	8	49	theme	HA	1613:1614	arg1	layers					1616:1621	HA layers	1613:1621	HA layers	1613:1621	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	7	50	theme	BMP-2	1531:1535	arg1	solution					1537:1544	BMP-2 solution	1531:1544	BMP-2 solution	1531:1544	After loading BMP-2 in CS, the BMP-2 can significantly improve cell adhesion, spreading and proliferation, and the loaded amount can also be controlled by the concentration of BMP-2 solution.					
31655887	8	51	with	material	1684:1691	arg1	properties					1737:1746	excellent biological and antibacterial properties	1698:1746	excellent biological and antibacterial properties	1698:1746	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	3	52	theme	coatings	722:729	arg1	behavior					620:627	BMP-2 release behavior	606:627	BMP-2 release behavior	606:627	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	3	52	theme	coatings	722:729	arg1	bioactivity					630:640	bioactivity	630:640	bioactivity	630:640	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	3	52	theme	coatings	722:729	arg1	biocompatibility					643:658	biocompatibility	643:658	biocompatibility	643:658	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	3	52	theme	coatings	722:729	arg1	activity					678:685	antibacterial activity	664:685	antibacterial activity	664:685	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	3	52	theme	coatings	722:729	arg1	degradability					585:597	The degradability	581:597	The degradability of CS	581:603	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	1	53	theme	-encapsulated	352:364	arg1	coatings					369:376	-encapsulated CS coatings	352:376	-encapsulated CS coatings	352:376	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	1	54	theme	titanium	225:232	arg1	surface					239:245	titanium (Ti) surface	225:245	titanium (Ti) surface	225:245	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	6	55	theme	cell	1253:1256	arg1	adhesion					1258:1265	cell adhesion	1253:1265	cell adhesion	1253:1265	All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF).					
31655887	4	56	theme	BMP-2	837:841	arg1	layer					808:812	the HA layer	801:812	the HA layer	801:812	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	4	56	theme	BMP-2	837:841	arg1	amount					850:855	the larger the loaded BMP-2 and CS amount	815:855	the larger the loaded BMP-2 and CS amount	815:855	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	5	57	theme	biological	1196:1205	arg1	property					1207:1214	biological property	1196:1214	biological property	1196:1214	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	3	58	theme	in	748:749	arg1	tests					757:761	in vitro tests	748:761	in vitro tests	748:761	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	5	59	theme	CS	992:993	arg1	concentration					995:1007	CS concentration	992:1007	CS concentration	992:1007	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	3	60	theme	CS	602:603	arg1	behavior					620:627	BMP-2 release behavior	606:627	BMP-2 release behavior	606:627	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	3	60	theme	CS	602:603	arg1	bioactivity					630:640	bioactivity	630:640	bioactivity	630:640	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	3	60	theme	CS	602:603	arg1	biocompatibility					643:658	biocompatibility	643:658	biocompatibility	643:658	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	3	60	theme	CS	602:603	arg1	activity					678:685	antibacterial activity	664:685	antibacterial activity	664:685	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	3	60	theme	CS	602:603	arg1	degradability					585:597	The degradability	581:597	The degradability of CS	581:603	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	8	61	theme	solutions	1658:1666	arg1	concentrations					1627:1640	concentrations	1627:1640	concentrations of CS and BMP-2 solutions	1627:1666	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	8	61	theme	solutions	1658:1666	arg1	thickness					1600:1608	the thickness	1596:1608	the thickness of HA layers	1596:1621	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	1	62	theme	petaling	147:154	arg1	coatings					191:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	petaling hydroxyapatite (HA)/TiO2 composite coatings	147:198	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	4	63	theme	CS	847:848	arg1	layer					808:812	the HA layer	801:812	the HA layer	801:812	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	4	63	theme	CS	847:848	arg1	amount					850:855	the larger the loaded BMP-2 and CS amount	815:855	the larger the loaded BMP-2 and CS amount	815:855	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	6	64	theme	simulated	1326:1334	arg1	SBF					1349:1351	SBF	1349:1351	SBF	1349:1351	All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF).					
31655887	6	64	theme	simulated	1326:1334	arg1	fluids					1341:1346	simulated body fluids	1326:1346	simulated body fluids (SBF)	1326:1352	All CS/HA/TiO2 coatings accelerated cell adhesion, spreading and proliferation, and promoted HA formation in simulated body fluids (SBF).					
31655887	1	65	theme	Ti	235:236	arg1	surface					239:245	titanium (Ti) surface	225:245	titanium (Ti) surface	225:245	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	0	66	theme	tissue	112:117	arg1	engineering					119:129	bone tissue engineering	107:129	bone tissue engineering	107:129	Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.					
31655887	8	67	theme	implant	1676:1682	arg1	material					1684:1691	the Ti implant material	1669:1691	the Ti implant material with excellent biological and antibacterial properties	1669:1746	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	7	68	theme	loaded	1470:1475	arg1	amount					1477:1482	the loaded amount	1466:1482	the loaded amount	1466:1482	After loading BMP-2 in CS, the BMP-2 can significantly improve cell adhesion, spreading and proliferation, and the loaded amount can also be controlled by the concentration of BMP-2 solution.					
31655887	1	69	theme	bone	318:321	arg1	protein-2					335:343	bone morphogenic protein-2	318:343	bone morphogenic protein-2 (BMP-2)	318:351	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	1	69	theme	bone	318:321	arg1	BMP-2					346:350	BMP-2	346:350	BMP-2	346:350	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	4	70	theme	bonding	878:884	arg1	strength					886:893	better bonding strength	871:893	better bonding strength between coatings	871:910	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	1	71	dep	chitosan	300:307	arg1	coatings					369:376	-encapsulated CS coatings	352:376	-encapsulated CS coatings	352:376	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	3	72	dep	in	748:749	arg1	vitro					751:755	vitro	751:755	vitro	751:755	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	8	73	theme	biological	1708:1717	arg1	properties					1737:1746	excellent biological and antibacterial properties	1698:1746	excellent biological and antibacterial properties	1698:1746	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	8	74	theme	antibacterial	1723:1735	arg1	properties					1737:1746	excellent biological and antibacterial properties	1698:1746	excellent biological and antibacterial properties	1698:1746	The present study indicates that, by controlling the thickness of HA layers and concentrations of CS and BMP-2 solutions, the Ti implant material with excellent biological and antibacterial properties can be achieved.					
31655887	5	75	theme	HA	1032:1033	arg1	coatings					1035:1042	HA coatings	1032:1042	HA coatings	1032:1042	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	2	76	theme	bonding	516:522	arg1	strength					524:531	The bonding strength	512:531	The bonding strength between coatings	512:548	The bonding strength between coatings was studied by scratch method.					
31655887	0	77	theme	antibacterial	45:57	arg1	coatings					73:80	BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings	15:80	BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings	15:80	Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.					
31655887	0	78	theme	coatings	73:80	arg1	Preparation					0:10	Preparation	0:10	Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.	0:130	Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.					
31655887	5	79	theme	CS	1112:1113	arg1	time					1127:1130	the prolonged CS degradation time	1098:1130	the prolonged CS degradation time	1098:1130	In addition, with the increase of CS concentration, more CS was loaded on HA coatings, which benefited the increase of CS degrading amount, the prolonged CS degradation time and BMP-2 release time, resulting in improved antibacterial and biological property.					
31655887	3	80	theme	BMP-2	606:610	arg1	behavior					620:627	BMP-2 release behavior	606:627	BMP-2 release behavior	606:627	The degradability of CS, BMP-2 release behavior, bioactivity, biocompatibility and antibacterial activity of the obtained (BMP-2)/CS/HA/TiO2 coatings were examined by in vitro tests.					
31655887	0	81	theme	titanium	85:92	arg1	surfaces					94:101	titanium surfaces	85:101	titanium surfaces for bone tissue engineering	85:129	Preparation of BMP-2/chitosan/hydroxyapatite antibacterial bio-composite coatings on titanium surfaces for bone tissue engineering.					
31655887	1	82	theme	pure	295:298	arg1	CS					310:311	CS	310:311	CS	310:311	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	1	82	theme	pure	295:298	arg1	chitosan					300:307	pure chitosan	295:307	pure chitosan (CS)	295:312	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	4	83	dep	showed	776:781	arg1	layer					808:812	the HA layer	801:812	the HA layer	801:812	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	4	83	dep	showed	776:781	arg1	biocompatibility					940:955	biocompatibility	940:955	biocompatibility	940:955	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	4	83	dep	showed	776:781	arg1	amount					850:855	the larger the loaded BMP-2 and CS amount	815:855	the larger the loaded BMP-2 and CS amount	815:855	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	4	83	dep	showed	776:781	arg1	activity					927:934	antibacterial activity	913:934	antibacterial activity	913:934	The results showed that, the thicker the HA layer, the larger the loaded BMP-2 and CS amount, resulting in better bonding strength between coatings, antibacterial activity and biocompatibility.					
31655887	1	84	theme	good	466:469	arg1	properties					500:509	good antibacterial and biological properties	466:509	good antibacterial and biological properties	466:509	In this paper, petaling hydroxyapatite (HA)/TiO2 composite coatings were firstly prepared on titanium (Ti) surface by one-step micro-arc oxidation (MAO), and then pure chitosan (CS) and bone morphogenic protein-2 (BMP-2)-encapsulated CS coatings were respectively loaded on the HA/TiO2 surfaces by dip-coating method to endow Ti with good antibacterial and biological properties.					
31655887	7	85	theme	cell	1418:1421	arg1	adhesion					1423:1430	cell adhesion	1418:1430	cell adhesion	1418:1430	After loading BMP-2 in CS, the BMP-2 can significantly improve cell adhesion, spreading and proliferation, and the loaded amount can also be controlled by the concentration of BMP-2 solution.					
30732796	1	0	dep	stability	292:300	arg1	100 °C					303:308	100 °C	303:308	100 °C for 30 min	303:319	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	7	1	theme	efficient	1129:1137	arg1	system					1139:1144	an efficient system	1126:1144	an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications	1126:1234	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	6	2	theme	activity	998:1005	arg1	terms					975:979	terms	975:979	terms of antibacterial activity	975:1005	The bacteriocins immobilized on CNC showed 50% increase in stability in terms of antibacterial activity.					
30732796	2	3	used	used	458:461	arg2	acidilactici					441:452	Pediococcus acidilactici	429:452	Pediococcus acidilactici	429:452	The bacteriocin was sensitive to protease treatment and most active in acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison.					
30732796	1	4	theme	Vigna	170:174	arg1	mungo					176:180	Vigna mungo	170:180	Vigna mungo (Black gram)	170:193	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	1	4	theme	Vigna	170:174	arg1	gram					189:192	Black gram	183:192	Black gram	183:192	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	6	5	theme	antibacterial	984:996	arg1	activity					998:1005	antibacterial activity	984:1005	antibacterial activity	984:1005	The bacteriocins immobilized on CNC showed 50% increase in stability in terms of antibacterial activity.					
30732796	1	6	theme	negative	258:265	arg1	bacteria					267:274	both Gram positive and Gram negative bacteria	230:274	both Gram positive and Gram negative bacteria	230:274	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	0	7	theme	cellulose	98:106	arg1	nanocrystals					108:119	cellulose nanocrystals	98:119	cellulose nanocrystals	98:119	Improving the stability of bacteriocin extracted from Enterococcus faecium by immobilization onto cellulose nanocrystals.					
30732796	1	8	attach	isolated	156:163	arg2	faecium					135:141	Enterococcus faecium	122:141	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram)	122:193	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	1	8	attach	isolated	156:163	arg1	mungo					176:180	Vigna mungo	170:180	Vigna mungo (Black gram)	170:193	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	1	8	attach	isolated	156:163	arg1	gram					189:192	Black gram	183:192	Black gram	183:192	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	4	9	theme	P.	702:703	arg1	acidilactici					705:716	P. acidilactici	702:716	P. acidilactici	702:716	The bacteriocin immobilization yield was 64.91% for P. acidilactici and 53.63% for E. faecium.					
30732796	1	10	dep	faecium	135:141	arg1	faecium					147:153	E. faecium	144:153	E. faecium	144:153	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	5	11	theme	chemical	863:870	arg1	nature					872:877	chemical nature	863:877	chemical nature	863:877	The bacteriocin immobilized CNC was characterized by DLS particle sizing, FTIR and AFM to evaluate size distribution, chemical nature and surface morphology.					
30732796	7	12	theme	immobilization	1079:1092	arg1	method					1094:1099	physical adsorption immobilization method	1059:1099	physical adsorption immobilization method for bacteriocin	1059:1115	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	4	13	theme	immobilization	666:679	arg1	yield					681:685	The bacteriocin immobilization yield	650:685	The bacteriocin immobilization yield	650:685	The bacteriocin immobilization yield was 64.91% for P. acidilactici and 53.63% for E. faecium.					
30732796	4	13	theme	immobilization	666:679	arg1	%					696:696	64.91%	691:696	64.91% for P. acidilactici	691:716	The bacteriocin immobilization yield was 64.91% for P. acidilactici and 53.63% for E. faecium.					
30732796	1	14	theme	Black	183:187	arg1	mungo					176:180	Vigna mungo	170:180	Vigna mungo (Black gram)	170:193	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	1	14	theme	Black	183:187	arg1	gram					189:192	Black gram	183:192	Black gram	183:192	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	2	15	theme	Pediococcus	429:439	arg1	acidilactici					441:452	Pediococcus acidilactici	429:452	Pediococcus acidilactici	429:452	The bacteriocin was sensitive to protease treatment and most active in acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison.					
30732796	7	16	with	combination	1042:1052	arg1	method					1094:1099	physical adsorption immobilization method	1059:1099	physical adsorption immobilization method for bacteriocin	1059:1115	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	1	17	theme	better	280:285	arg1	stability					292:300	better heat stability	280:300	better heat stability (100 °C for 30 min)	280:320	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	7	18	theme	adsorption	1068:1077	arg1	method					1094:1099	physical adsorption immobilization method	1059:1099	physical adsorption immobilization method for bacteriocin	1059:1115	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	7	19	theme	food	1189:1192	arg1	packaging					1194:1202	food packaging	1189:1202	food packaging	1189:1202	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	1	20	theme	heat	287:290	arg1	stability					292:300	better heat stability	280:300	better heat stability (100 °C for 30 min)	280:320	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
30732796	6	21	from	increase	950:957	arg1	terms					975:979	terms	975:979	terms of antibacterial activity	975:1005	The bacteriocins immobilized on CNC showed 50% increase in stability in terms of antibacterial activity.					
30732796	6	21	from	increase	950:957	arg1	stability					962:970	stability	962:970	stability	962:970	The bacteriocins immobilized on CNC showed 50% increase in stability in terms of antibacterial activity.					
30732796	3	22	theme	physical	565:572	arg1	adsorption					574:583	physical adsorption	565:583	physical adsorption onto cellulose nanocrystals (CNC) extracted from cotton linters	565:647	To enhance stability for diversified applications, the bacteriocin was immobilized by physical adsorption onto cellulose nanocrystals (CNC) extracted from cotton linters.					
30732796	2	23	theme	pH.	401:403	arg1	Bacteriocin					405:415	acidic pH. Bacteriocin	394:415	acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison	394:476	The bacteriocin was sensitive to protease treatment and most active in acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison.					
30732796	7	24	theme	antibacterial	1159:1171	arg1	nanofillers					1173:1183	antibacterial nanofillers	1159:1183	antibacterial nanofillers	1159:1183	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	4	25	theme	bacteriocin	654:664	arg1	yield					681:685	The bacteriocin immobilization yield	650:685	The bacteriocin immobilization yield	650:685	The bacteriocin immobilization yield was 64.91% for P. acidilactici and 53.63% for E. faecium.					
30732796	4	25	theme	bacteriocin	654:664	arg1	%					696:696	64.91%	691:696	64.91% for P. acidilactici	691:716	The bacteriocin immobilization yield was 64.91% for P. acidilactici and 53.63% for E. faecium.					
30732796	2	26	theme	acidic	394:399	arg1	Bacteriocin					405:415	acidic pH. Bacteriocin	394:415	acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison	394:476	The bacteriocin was sensitive to protease treatment and most active in acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison.					
30732796	0	27	theme	bacteriocin	27:37	arg1	stability					14:22	the stability	10:22	the stability of bacteriocin extracted from Enterococcus faecium by immobilization onto cellulose nanocrystals	10:119	Improving the stability of bacteriocin extracted from Enterococcus faecium by immobilization onto cellulose nanocrystals.					
30732796	6	28	theme	%	948:948	arg1	increase					950:957	50% increase	946:957	50% increase in stability in terms of antibacterial activity	946:1005	The bacteriocins immobilized on CNC showed 50% increase in stability in terms of antibacterial activity.					
30732796	7	29	theme	physical	1059:1066	arg1	method					1094:1099	physical adsorption immobilization method	1059:1099	physical adsorption immobilization method for bacteriocin	1059:1115	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	7	30	theme	enzymatic	1012:1020	arg1	synthesis					1022:1030	The enzymatic synthesis	1008:1030	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin	1008:1115	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	5	31	theme	size	844:847	arg1	distribution					849:860	size distribution	844:860	size distribution	844:860	The bacteriocin immobilized CNC was characterized by DLS particle sizing, FTIR and AFM to evaluate size distribution, chemical nature and surface morphology.					
30732796	0	32	theme	Enterococcus	54:65	arg1	faecium					67:73	Enterococcus faecium	54:73	Enterococcus faecium	54:73	Improving the stability of bacteriocin extracted from Enterococcus faecium by immobilization onto cellulose nanocrystals.					
30732796	6	33	theme	50	946:947	arg1	%					948:948	%	948:948	%	948:948	The bacteriocins immobilized on CNC showed 50% increase in stability in terms of antibacterial activity.					
30732796	5	34	theme	immobilized	761:771	arg1	CNC					773:775	The bacteriocin immobilized CNC	745:775	The bacteriocin immobilized CNC	745:775	The bacteriocin immobilized CNC was characterized by DLS particle sizing, FTIR and AFM to evaluate size distribution, chemical nature and surface morphology.					
30732796	3	35	theme	cellulose	590:598	arg1	CNC					614:616	CNC	614:616	CNC	614:616	To enhance stability for diversified applications, the bacteriocin was immobilized by physical adsorption onto cellulose nanocrystals (CNC) extracted from cotton linters.					
30732796	3	35	theme	cellulose	590:598	arg1	nanocrystals					600:611	cellulose nanocrystals	590:611	cellulose nanocrystals (CNC) extracted from cotton linters	590:647	To enhance stability for diversified applications, the bacteriocin was immobilized by physical adsorption onto cellulose nanocrystals (CNC) extracted from cotton linters.					
30732796	5	36	theme	particle	802:809	arg1	sizing					811:816	DLS particle sizing	798:816	DLS particle sizing	798:816	The bacteriocin immobilized CNC was characterized by DLS particle sizing, FTIR and AFM to evaluate size distribution, chemical nature and surface morphology.					
30732796	2	37	from	active	384:389	arg1	Bacteriocin					405:415	acidic pH. Bacteriocin	394:415	acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison	394:476	The bacteriocin was sensitive to protease treatment and most active in acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison.					
30732796	3	38	theme	diversified	504:514	arg1	applications					516:527	diversified applications	504:527	diversified applications	504:527	To enhance stability for diversified applications, the bacteriocin was immobilized by physical adsorption onto cellulose nanocrystals (CNC) extracted from cotton linters.					
30732796	7	39	theme	CNC	1035:1037	arg1	synthesis					1022:1030	The enzymatic synthesis	1008:1030	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin	1008:1115	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	3	40	theme	cotton	634:639	arg1	linters					641:647	cotton linters	634:647	cotton linters	634:647	To enhance stability for diversified applications, the bacteriocin was immobilized by physical adsorption onto cellulose nanocrystals (CNC) extracted from cotton linters.					
30732796	7	41	from	CNC	1035:1037	arg1	combination					1042:1052	combination	1042:1052	combination with physical adsorption immobilization method for bacteriocin	1042:1115	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	5	42	theme	bacteriocin	749:759	arg1	CNC					773:775	The bacteriocin immobilized CNC	745:775	The bacteriocin immobilized CNC	745:775	The bacteriocin immobilized CNC was characterized by DLS particle sizing, FTIR and AFM to evaluate size distribution, chemical nature and surface morphology.					
30732796	7	43	from	synthesis	1022:1030	arg1	combination					1042:1052	combination	1042:1052	combination with physical adsorption immobilization method for bacteriocin	1042:1115	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	2	44	theme	protease	356:363	arg1	treatment					365:373	protease treatment	356:373	protease treatment	356:373	The bacteriocin was sensitive to protease treatment and most active in acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison.					
30732796	5	45	theme	DLS	798:800	arg1	sizing					811:816	DLS particle sizing	798:816	DLS particle sizing	798:816	The bacteriocin immobilized CNC was characterized by DLS particle sizing, FTIR and AFM to evaluate size distribution, chemical nature and surface morphology.					
30732796	7	46	theme	bio-composites	1208:1221	arg1	applications					1223:1234	bio-composites applications	1208:1234	bio-composites applications	1208:1234	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	7	47	from	combination	1042:1052	arg1	synthesis					1022:1030	The enzymatic synthesis	1008:1030	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin	1008:1115	The enzymatic synthesis of CNC in combination with physical adsorption immobilization method for bacteriocin makes it an efficient system of producing antibacterial nanofillers for food packaging and bio-composites applications.					
30732796	2	48	from	Bacteriocin	405:415	arg1	active					384:389	active	384:389	active	384:389	The bacteriocin was sensitive to protease treatment and most active in acidic pH. Bacteriocin produced by Pediococcus acidilactici was used for comparison.					
30732796	5	49	theme	surface	883:889	arg1	morphology					891:900	surface morphology	883:900	surface morphology	883:900	The bacteriocin immobilized CNC was characterized by DLS particle sizing, FTIR and AFM to evaluate size distribution, chemical nature and surface morphology.					
30732796	1	50	theme	positive	240:247	arg1	bacteria					267:274	both Gram positive and Gram negative bacteria	230:274	both Gram positive and Gram negative bacteria	230:274	Enterococcus faecium (E. faecium) isolated from Vigna mungo (Black gram) produced bacteriocin that inhibits both Gram positive and Gram negative bacteria and better heat stability (100 °C for 30 min).					
29617123	7	0	theme	succinic	1458:1465	arg1	semialdehyde					1467:1478	succinic semialdehyde	1458:1478	succinic semialdehyde	1458:1478	Additionally, 13C flux from α-ketoglutarate to succinate is demonstrated to occur via succinic semialdehyde, an alternative to the conventional TCA cycle, in Synechococcus 7002 under photoautotrophic conditions.					
29617123	7	0	theme	succinic	1458:1465	arg1	alternative					1484:1494	an alternative	1481:1494	an alternative to the conventional TCA cycle	1481:1524	Additionally, 13C flux from α-ketoglutarate to succinate is demonstrated to occur via succinic semialdehyde, an alternative to the conventional TCA cycle, in Synechococcus 7002 under photoautotrophic conditions.					
29617123	3	1	theme	13C-isotopomer	678:691	arg1	fluxes					693:698	measured 13C-isotopomer fluxes	669:698	measured 13C-isotopomer fluxes	669:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	1	2	theme	engineering	263:273	arg1	goals					249:253	the preeminent goals	234:253	the preeminent goals of gene engineering of photoautotrophs	234:292	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	1	2	theme	engineering	263:273	arg1	rates					170:174	Boosting cellular growth rates	145:174	Boosting cellular growth rates while redirecting metabolism to make desired products	145:228	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	8	3	theme	acetyl	1694:1699	arg1	coenzyme-A					1701:1710	acetyl coenzyme-A	1694:1710	acetyl coenzyme-A	1694:1710	We found that pyruvate and oxaloacetate are synthesized mainly by malate dehydrogenase with minimal flux into acetyl coenzyme-A via pyruvate dehydrogenase.					
29617123	1	4	theme	photoautotrophs	278:292	arg1	engineering					263:273	gene engineering	258:273	gene engineering of photoautotrophs	258:292	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	7	5	theme	13C	1386:1388	arg1	flux					1390:1393	13C flux	1386:1393	13C flux from α-ketoglutarate to succinate	1386:1427	Additionally, 13C flux from α-ketoglutarate to succinate is demonstrated to occur via succinic semialdehyde, an alternative to the conventional TCA cycle, in Synechococcus 7002 under photoautotrophic conditions.					
29617123	6	6	theme	biomass	1234:1240	arg1	accumulation					1242:1253	biomass accumulation	1234:1253	biomass accumulation under N starvation	1234:1272	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	5	7	dep	increased	999:1007	arg1	1					996:996	1	996:996	1	996:996	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	3	8	theme	residuals	733:741	arg1	sum					718:720	the sum	714:720	the sum of squared residuals between experimental and predicted mass isotopomers	714:793	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	5	9	theme	→	1064:1064	arg1	GAP/DHAP					1066:1073	3PG → GAP/DHAP	1060:1073	3PG → GAP/DHAP	1060:1073	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	5	9	theme	→	1064:1064	arg1	gluconeogenesis					1043:1057	gluconeogenesis	1043:1057	gluconeogenesis (3PG → GAP/DHAP)	1043:1074	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	1	10	theme	desired	213:219	arg1	products					221:228	desired products	213:228	desired products	213:228	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	6	11	theme	growth	1218:1223	arg1	rate					1225:1228	the slower growth rate	1207:1228	the slower growth rate	1207:1228	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	6	12	theme	carbon	1294:1299	arg1	rate					1310:1313	a reduced carbon fixation rate	1284:1313	a reduced carbon fixation rate	1284:1313	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	1	13	theme	pathways	393:400	arg1	understanding					361:373	understanding	361:373	understanding of the functional pathways and their choke points	361:423	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	3	14	theme	predicted	768:776	arg1	isotopomers					783:793	predicted mass isotopomers	768:793	predicted mass isotopomers	768:793	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	1	15	theme	points	418:423	arg1	understanding					361:373	understanding	361:373	understanding of the functional pathways and their choke points	361:423	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	5	16	theme	glycogen	936:943	arg1	synthesis					945:953	glycogen synthesis	936:953	glycogen synthesis	936:953	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	6	17	theme	amino	1349:1353	arg1	precursors					1360:1369	amino acid precursors	1349:1369	amino acid precursors	1349:1369	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	5	18	theme	flux	1085:1088	arg1	overflow					1090:1097	(2) flux overflow	1081:1097	(2) flux overflow	1081:1097	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	3	19	theme	absolute	612:619	arg1	fluxes					621:626	absolute fluxes	612:626	absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes	612:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	1	20	theme	cellular	154:161	arg1	goals					249:253	the preeminent goals	234:253	the preeminent goals of gene engineering of photoautotrophs	234:292	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	1	20	theme	cellular	154:161	arg1	rates					170:174	Boosting cellular growth rates	145:174	Boosting cellular growth rates while redirecting metabolism to make desired products	145:228	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	9	21	theme	major	1764:1768	arg1	shifts					1770:1775	major shifts	1764:1775	major shifts	1764:1775	Nutrient stress induces major shifts in fluxes into new pathways that deviate from historical metabolic pathways derived from model bacteria.					
29617123	6	22	theme	carbon	1337:1342	arg1	flux					1329:1332	a reduced flux	1319:1332	a reduced flux of carbon into amino acid precursors	1319:1369	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	6	22	theme	carbon	1337:1342	arg1	rate					1310:1313	a reduced carbon fixation rate	1284:1313	a reduced carbon fixation rate	1284:1313	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	2	23	theme	instantaneous	490:502	arg1	fluxes					504:509	instantaneous fluxes	490:509	instantaneous fluxes of metabolites	490:524	Here we apply a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth.					
29617123	0	24	theme	Selected	93:100	arg1	Pathways					102:109	the Selected Pathways	89:109	the Selected Pathways in Cyanobacterial Photosynthesis	89:142	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes Reveal the Selected Pathways in Cyanobacterial Photosynthesis.					
29617123	1	25	theme	understanding	361:373	arg1	lack					353:356	lack	353:356	lack of understanding of the functional pathways and their choke points	353:423	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	0	26	theme	Rerouting	0:8	arg1	Fluxes					75:80	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes	0:80	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes	0:80	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes Reveal the Selected Pathways in Cyanobacterial Photosynthesis.					
29617123	5	27	theme	phosphate	1164:1172	arg1	pathway					1181:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	0	28	theme	Metabolism	13:22	arg1	Rerouting					0:8	Rerouting	0:8	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes	0:80	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes Reveal the Selected Pathways in Cyanobacterial Photosynthesis.					
29617123	5	29	theme	hGPP	1175:1178	arg1	pathway					1181:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	7	30	theme	conventional	1503:1514	arg1	cycle					1520:1524	the conventional TCA cycle	1499:1524	the conventional TCA cycle	1499:1524	Additionally, 13C flux from α-ketoglutarate to succinate is demonstrated to occur via succinic semialdehyde, an alternative to the conventional TCA cycle, in Synechococcus 7002 under photoautotrophic conditions.					
29617123	9	31	theme	historical	1823:1832	arg1	pathways					1844:1851	historical metabolic pathways	1823:1851	historical metabolic pathways derived from model bacteria	1823:1879	Nutrient stress induces major shifts in fluxes into new pathways that deviate from historical metabolic pathways derived from model bacteria.					
29617123	5	32	dep	overflow	1090:1097	arg1	2					1082:1082	2	1082:1082	2	1082:1082	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	4	33	theme	observed	820:827	arg1	shift					829:833	the widely observed shift	809:833	the widely observed shift in biomass composition in cyanobacteria	809:873	We show that the widely observed shift in biomass composition in cyanobacteria, demonstrated here with Synechococcus sp.					
29617123	0	34	theme	Desired	29:35	arg1	Products					46:53	Desired Cellular Products	29:53	Desired Cellular Products	29:53	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes Reveal the Selected Pathways in Cyanobacterial Photosynthesis.					
29617123	9	35	theme	metabolic	1834:1842	arg1	pathways					1844:1851	historical metabolic pathways	1823:1851	historical metabolic pathways derived from model bacteria	1823:1879	Nutrient stress induces major shifts in fluxes into new pathways that deviate from historical metabolic pathways derived from model bacteria.					
29617123	2	36	theme	mass	446:449	arg1	INST-MFA					468:475	INST-MFA	468:475	INST-MFA	468:475	Here we apply a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth.					
29617123	2	36	theme	mass	446:449	arg1	method					460:465	a 13C mass isotopic method	440:465	a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth	440:555	Here we apply a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth.					
29617123	0	37	theme	Nutrient	58:65	arg1	Stress					67:72	Nutrient Stress	58:72	Nutrient Stress	58:72	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes Reveal the Selected Pathways in Cyanobacterial Photosynthesis.					
29617123	5	38	theme	hybrid	1133:1138	arg1	pathway					1181:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	3	39	theme	measured	669:676	arg1	fluxes					693:698	measured 13C-isotopomer fluxes	669:698	measured 13C-isotopomer fluxes	669:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	1	40	theme	gene	258:261	arg1	engineering					263:273	gene engineering	258:273	gene engineering of photoautotrophs	258:292	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	3	41	theme	fluxes	693:698	arg1	set					662:664	a finite set	653:664	a finite set of measured 13C-isotopomer fluxes	653:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	3	41	theme	fluxes	693:698	arg1	fluxes					693:698	measured 13C-isotopomer fluxes	669:698	measured 13C-isotopomer fluxes	669:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	6	42	theme	N	1261:1261	arg1	starvation					1263:1272	N starvation	1261:1272	N starvation	1261:1272	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	8	43	theme	minimal	1676:1682	arg1	flux					1684:1687	minimal flux	1676:1687	minimal flux into acetyl coenzyme-A via pyruvate dehydrogenase	1676:1737	We found that pyruvate and oxaloacetate are synthesized mainly by malate dehydrogenase with minimal flux into acetyl coenzyme-A via pyruvate dehydrogenase.					
29617123	3	44	theme	squared	725:731	arg1	residuals					733:741	squared residuals	725:741	squared residuals between experimental and predicted mass isotopomers	725:793	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	4	45	theme	biomass	838:844	arg1	composition					846:856	biomass composition	838:856	biomass composition	838:856	We show that the widely observed shift in biomass composition in cyanobacteria, demonstrated here with Synechococcus sp.					
29617123	0	46	theme	Cyanobacterial	114:127	arg1	Photosynthesis					129:142	Cyanobacterial Photosynthesis	114:142	Cyanobacterial Photosynthesis	114:142	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes Reveal the Selected Pathways in Cyanobacterial Photosynthesis.					
29617123	8	47	theme	pyruvate	1716:1723	arg1	dehydrogenase					1725:1737	pyruvate dehydrogenase	1716:1737	pyruvate dehydrogenase	1716:1737	We found that pyruvate and oxaloacetate are synthesized mainly by malate dehydrogenase with minimal flux into acetyl coenzyme-A via pyruvate dehydrogenase.					
29617123	2	48	theme	photoautotrophic	533:548	arg1	growth					550:555	photoautotrophic growth	533:555	photoautotrophic growth	533:555	Here we apply a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth.					
29617123	6	49	theme	fixation	1301:1308	arg1	rate					1310:1313	a reduced carbon fixation rate	1284:1313	a reduced carbon fixation rate	1284:1313	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	1	50	theme	functional	382:391	arg1	pathways					393:400	the functional pathways	378:400	the functional pathways	378:400	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	3	51	theme	accurate	596:603	arg1	set					605:607	the globally most accurate set	578:607	the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes	578:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	3	51	theme	accurate	596:603	arg1	fluxes					621:626	absolute fluxes	612:626	absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes	612:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	2	52	theme	metabolites	514:524	arg1	fluxes					504:509	instantaneous fluxes	490:509	instantaneous fluxes of metabolites	490:524	Here we apply a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth.					
29617123	6	53	theme	slower	1211:1216	arg1	rate					1225:1228	the slower growth rate	1207:1228	the slower growth rate	1207:1228	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	6	54	theme	reduced	1286:1292	arg1	rate					1310:1313	a reduced carbon fixation rate	1284:1313	a reduced carbon fixation rate	1284:1313	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	4	55	from	shift	829:833	arg1	cyanobacteria					861:873	cyanobacteria	861:873	cyanobacteria	861:873	We show that the widely observed shift in biomass composition in cyanobacteria, demonstrated here with Synechococcus sp.					
29617123	4	55	from	shift	829:833	arg1	composition					846:856	biomass composition	838:856	biomass composition	838:856	We show that the widely observed shift in biomass composition in cyanobacteria, demonstrated here with Synechococcus sp.					
29617123	4	56	theme	Synechococcus	899:911	arg1	sp					913:914	Synechococcus sp	899:914	Synechococcus sp	899:914	We show that the widely observed shift in biomass composition in cyanobacteria, demonstrated here with Synechococcus sp.					
29617123	0	57	from	Pathways	102:109	arg1	Photosynthesis					129:142	Cyanobacterial Photosynthesis	114:142	Cyanobacterial Photosynthesis	114:142	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes Reveal the Selected Pathways in Cyanobacterial Photosynthesis.					
29617123	3	58	theme	mass	778:781	arg1	isotopomers					783:793	predicted mass isotopomers	768:793	predicted mass isotopomers	768:793	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	1	59	theme	choke	412:416	arg1	points					418:423	their choke points	406:423	their choke points	406:423	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	3	60	theme	fluxes	621:626	arg1	set					605:607	the globally most accurate set	578:607	the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes	578:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	3	60	theme	fluxes	621:626	arg1	fluxes					621:626	absolute fluxes	612:626	absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes	612:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	7	61	theme	photoautotrophic	1555:1570	arg1	conditions					1572:1581	photoautotrophic conditions	1555:1581	photoautotrophic conditions	1555:1581	Additionally, 13C flux from α-ketoglutarate to succinate is demonstrated to occur via succinic semialdehyde, an alternative to the conventional TCA cycle, in Synechococcus 7002 under photoautotrophic conditions.					
29617123	9	62	theme	Nutrient	1740:1747	arg1	stress					1749:1754	Nutrient stress	1740:1754	Nutrient stress	1740:1754	Nutrient stress induces major shifts in fluxes into new pathways that deviate from historical metabolic pathways derived from model bacteria.					
29617123	5	63	from	step	1035:1038	arg1	overflow					1090:1097	(2) flux overflow	1081:1097	(2) flux overflow	1081:1097	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	5	63	from	step	1035:1038	arg1	gluconeogenesis					1043:1057	gluconeogenesis	1043:1057	gluconeogenesis (3PG → GAP/DHAP)	1043:1074	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	5	63	from	step	1035:1038	arg1	GAP/DHAP					1066:1073	3PG → GAP/DHAP	1060:1073	3PG → GAP/DHAP	1060:1073	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	6	64	theme	acid	1355:1358	arg1	precursors					1360:1369	amino acid precursors	1349:1369	amino acid precursors	1349:1369	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	5	65	theme	3PG	1060:1062	arg1	GAP/DHAP					1066:1073	3PG → GAP/DHAP	1060:1073	3PG → GAP/DHAP	1060:1073	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	5	65	theme	3PG	1060:1062	arg1	gluconeogenesis					1043:1057	gluconeogenesis	1043:1057	gluconeogenesis (3PG → GAP/DHAP)	1043:1074	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	9	66	attach	derived	1853:1859	arg1	bacteria					1872:1879	model bacteria	1866:1879	model bacteria	1866:1879	Nutrient stress induces major shifts in fluxes into new pathways that deviate from historical metabolic pathways derived from model bacteria.					
29617123	9	66	attach	derived	1853:1859	arg2	pathways					1844:1851	historical metabolic pathways	1823:1851	historical metabolic pathways derived from model bacteria	1823:1879	Nutrient stress induces major shifts in fluxes into new pathways that deviate from historical metabolic pathways derived from model bacteria.					
29617123	1	67	theme	Boosting	145:152	arg1	goals					249:253	the preeminent goals	234:253	the preeminent goals of gene engineering of photoautotrophs	234:292	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	1	67	theme	Boosting	145:152	arg1	rates					170:174	Boosting cellular growth rates	145:174	Boosting cellular growth rates while redirecting metabolism to make desired products	145:228	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	6	68	dep	suggest	1199:1205	arg1	due					1277:1279	due	1277:1279	due	1277:1279	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	1	69	theme	growth	163:168	arg1	goals					249:253	the preeminent goals	234:253	the preeminent goals of gene engineering of photoautotrophs	234:292	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	1	69	theme	growth	163:168	arg1	rates					170:174	Boosting cellular growth rates	145:174	Boosting cellular growth rates while redirecting metabolism to make desired products	145:228	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	7	70	from	α-ketoglutarate	1400:1414	arg1	flux					1390:1393	13C flux	1386:1393	13C flux from α-ketoglutarate to succinate	1386:1427	Additionally, 13C flux from α-ketoglutarate to succinate is demonstrated to occur via succinic semialdehyde, an alternative to the conventional TCA cycle, in Synechococcus 7002 under photoautotrophic conditions.					
29617123	6	71	theme	reduced	1321:1327	arg1	flux					1329:1332	a reduced flux	1319:1332	a reduced flux of carbon into amino acid precursors	1319:1369	Our data suggest the slower growth rate and biomass accumulation under N starvation is due to a reduced carbon fixation rate and a reduced flux of carbon into amino acid precursors.					
29617123	9	72	theme	new	1792:1794	arg1	pathways					1796:1803	new pathways	1792:1803	new pathways that deviate from historical metabolic pathways derived from model bacteria	1792:1879	Nutrient stress induces major shifts in fluxes into new pathways that deviate from historical metabolic pathways derived from model bacteria.					
29617123	5	73	theme	nitrogen	962:969	arg1	starvation					971:980	nitrogen starvation	962:980	nitrogen starvation	962:980	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	0	74	theme	Cellular	37:44	arg1	Products					46:53	Desired Cellular Products	29:53	Desired Cellular Products	29:53	Rerouting of Metabolism into Desired Cellular Products by Nutrient Stress: Fluxes Reveal the Selected Pathways in Cyanobacterial Photosynthesis.					
29617123	7	75	theme	TCA	1516:1518	arg1	cycle					1520:1524	the conventional TCA cycle	1499:1524	the conventional TCA cycle	1499:1524	Additionally, 13C flux from α-ketoglutarate to succinate is demonstrated to occur via succinic semialdehyde, an alternative to the conventional TCA cycle, in Synechococcus 7002 under photoautotrophic conditions.					
29617123	2	76	theme	isotopic	451:458	arg1	INST-MFA					468:475	INST-MFA	468:475	INST-MFA	468:475	Here we apply a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth.					
29617123	2	76	theme	isotopic	451:458	arg1	method					460:465	a 13C mass isotopic method	440:465	a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth	440:555	Here we apply a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth.					
29617123	5	77	theme	unrecognized	1120:1131	arg1	pathway					1181:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	5	78	theme	bottleneck	1024:1033	arg1	step					1035:1038	a bottleneck step	1022:1038	a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow	1022:1097	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	2	79	theme	13C	442:444	arg1	INST-MFA					468:475	INST-MFA	468:475	INST-MFA	468:475	Here we apply a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth.					
29617123	2	79	theme	13C	442:444	arg1	method					460:465	a 13C mass isotopic method	440:465	a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth	440:555	Here we apply a 13C mass isotopic method (INST-MFA) to quantify instantaneous fluxes of metabolites during photoautotrophic growth.					
29617123	5	80	theme	gluconeogenesis-pentose	1140:1162	arg1	pathway					1181:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway	1107:1187	PCC 7002, favoring glycogen synthesis during nitrogen starvation is caused by (1) increased flux through a bottleneck step in gluconeogenesis (3PG → GAP/DHAP), and (2) flux overflow through a previously unrecognized hybrid gluconeogenesis-pentose phosphate (hGPP) pathway.					
29617123	9	81	theme	model	1866:1870	arg1	bacteria					1872:1879	model bacteria	1866:1879	model bacteria	1866:1879	Nutrient stress induces major shifts in fluxes into new pathways that deviate from historical metabolic pathways derived from model bacteria.					
29617123	8	82	with	dehydrogenase	1657:1669	arg1	flux					1684:1687	minimal flux	1676:1687	minimal flux into acetyl coenzyme-A via pyruvate dehydrogenase	1676:1737	We found that pyruvate and oxaloacetate are synthesized mainly by malate dehydrogenase with minimal flux into acetyl coenzyme-A via pyruvate dehydrogenase.					
29617123	3	83	theme	finite	655:660	arg1	set					662:664	a finite set	653:664	a finite set of measured 13C-isotopomer fluxes	653:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	3	83	theme	finite	655:660	arg1	fluxes					693:698	measured 13C-isotopomer fluxes	669:698	measured 13C-isotopomer fluxes	669:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
29617123	8	84	theme	malate	1650:1655	arg1	dehydrogenase					1657:1669	malate dehydrogenase	1650:1669	malate dehydrogenase with minimal flux into acetyl coenzyme-A via pyruvate dehydrogenase	1650:1737	We found that pyruvate and oxaloacetate are synthesized mainly by malate dehydrogenase with minimal flux into acetyl coenzyme-A via pyruvate dehydrogenase.					
29617123	1	85	theme	preeminent	238:247	arg1	goals					249:253	the preeminent goals	234:253	the preeminent goals of gene engineering of photoautotrophs	234:292	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	1	85	theme	preeminent	238:247	arg1	rates					170:174	Boosting cellular growth rates	145:174	Boosting cellular growth rates while redirecting metabolism to make desired products	145:228	Boosting cellular growth rates while redirecting metabolism to make desired products are the preeminent goals of gene engineering of photoautotrophs, yet so far these goals have been hardly achieved owing to lack of understanding of the functional pathways and their choke points.					
29617123	3	86	from	set	662:664	arg1	metabolite					637:646	each metabolite	632:646	each metabolite from a finite set of measured 13C-isotopomer fluxes	632:698	INST-MFA determines the globally most accurate set of absolute fluxes for each metabolite from a finite set of measured 13C-isotopomer fluxes by minimizing the sum of squared residuals between experimental and predicted mass isotopomers.					
30171979	2	0	theme	formulation	443:453	arg1	approach					455:462	a novel formulation approach	435:462	a novel formulation approach based on non-uniform distribution of the active ingredient throughout the swellable polymer matrix	435:561	In order to solve this issue, a novel formulation approach based on non-uniform distribution of the active ingredient throughout the swellable polymer matrix was proposed and evaluated.					
30171979	6	1	contain	having	1088:1093	arg2	distribution					1103:1114	uniform distribution	1095:1114	uniform distribution of the drug tracer	1095:1133	As compared with a system having uniform distribution of the drug tracer, the multi-layer formulations were proved to enhance linearity of release.					
30171979	6	1	contain	having	1088:1093	arg1	system					1081:1086	a system	1079:1086	a system having uniform distribution of the drug tracer	1079:1133	As compared with a system having uniform distribution of the drug tracer, the multi-layer formulations were proved to enhance linearity of release.					
30171979	4	2	theme	physico-technological	857:877	arg1	characteristics					879:893	satisfactory physico-technological characteristics	844:893	satisfactory physico-technological characteristics	844:893	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	3	3	theme	decreasing	698:707	arg1	concentrations					709:722	decreasing concentrations	698:722	decreasing concentrations of the latter	698:736	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	1	4	theme	progressive	245:255	arg1	increase					257:264	progressive increase	245:264	progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front	245:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	0	5	theme	release	82:88	arg1	kinetics					90:97	zero-order release kinetics	71:97	zero-order release kinetics	71:97	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics.					
30171979	1	6	dep	distance	273:280	arg1	have					301:304	have	301:304	have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front	301:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	4	7	theme	satisfactory	844:855	arg1	characteristics					879:893	satisfactory physico-technological characteristics	844:893	satisfactory physico-technological characteristics	844:893	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	6	8	theme	multi-layer	1140:1150	arg1	formulations					1152:1163	the multi-layer formulations	1136:1163	the multi-layer formulations	1136:1163	As compared with a system having uniform distribution of the drug tracer, the multi-layer formulations were proved to enhance linearity of release.					
30171979	4	9	theme	consistent	921:930	arg1	thickness					932:940	consistent thickness	921:940	consistent thickness	921:940	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	7	10	theme	hydrophilic	1462:1472	arg1	systems					1481:1487	hydrophilic matrix systems	1462:1487	hydrophilic matrix systems	1462:1487	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	4	11	theme	applied	969:975	arg1	layers					949:954	the layers	945:954	the layers sequentially applied	945:975	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	4	12	theme	layers	949:954	arg1	shape					911:915	spherical shape	901:915	spherical shape	901:915	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	4	12	theme	layers	949:954	arg1	thickness					932:940	consistent thickness	921:940	consistent thickness	921:940	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	3	13	theme	tracer	667:672	arg1	acetaminophen					675:687	drug tracer (acetaminophen)	662:688	drug tracer (acetaminophen)	662:688	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	6	14	theme	release	1201:1207	arg1	linearity					1188:1196	linearity	1188:1196	linearity of release	1188:1207	As compared with a system having uniform distribution of the drug tracer, the multi-layer formulations were proved to enhance linearity of release.					
30171979	5	15	theme	non-uniform	982:992	arg1	composition					1001:1011	The non-uniform matrix composition	978:1011	The non-uniform matrix composition pursued	978:1019	The non-uniform matrix composition pursued was confirmed by Raman mapping analysis.					
30171979	7	16	theme	high-impact	1292:1302	arg1	operations					1311:1320	high-impact drying operations	1292:1320	high-impact drying operations	1292:1320	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	2	17	theme	non-uniform	473:483	arg1	distribution					485:496	non-uniform distribution	473:496	non-uniform distribution of the active ingredient throughout the swellable polymer matrix	473:561	In order to solve this issue, a novel formulation approach based on non-uniform distribution of the active ingredient throughout the swellable polymer matrix was proposed and evaluated.					
30171979	7	18	theme	drying	1304:1309	arg1	operations					1311:1320	high-impact drying operations	1292:1320	high-impact drying operations	1292:1320	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	5	19	theme	matrix	994:999	arg1	composition					1001:1011	The non-uniform matrix composition	978:1011	The non-uniform matrix composition pursued	978:1019	The non-uniform matrix composition pursued was confirmed by Raman mapping analysis.					
30171979	7	20	theme	established	1353:1363	arg1	use					1365:1367	established use	1353:1367	established use	1353:1367	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	1	21	from	outwards	326:333	arg1	front					398:402	the swelling front	385:402	the swelling front	385:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	1	22	theme	area	356:359	arg1	reduction					339:347	reduction	339:347	reduction	339:347	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	1	22	theme	area	356:359	arg1	outwards					326:333	outwards	326:333	outwards	326:333	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	6	23	theme	uniform	1095:1101	arg1	distribution					1103:1114	uniform distribution	1095:1114	uniform distribution of the drug tracer	1095:1133	As compared with a system having uniform distribution of the drug tracer, the multi-layer formulations were proved to enhance linearity of release.					
30171979	0	24	theme	hydrophilic	6:16	arg1	system					25:30	Novel hydrophilic matrix system	0:30	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics	0:97	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics.					
30171979	2	25	theme	novel	437:441	arg1	approach					455:462	a novel formulation approach	435:462	a novel formulation approach based on non-uniform distribution of the active ingredient throughout the swellable polymer matrix	435:561	In order to solve this issue, a novel formulation approach based on non-uniform distribution of the active ingredient throughout the swellable polymer matrix was proposed and evaluated.					
30171979	7	26	theme	advantageous	1237:1248	arg1	concept					1228:1234	The simple design concept	1210:1234	The simple design concept	1210:1234	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	7	26	theme	advantageous	1237:1248	arg1	technique					1250:1258	advantageous technique	1237:1258	advantageous technique	1237:1258	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	0	27	theme	zero-order	71:80	arg1	kinetics					90:97	zero-order release kinetics	71:97	zero-order release kinetics	71:97	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics.					
30171979	0	28	theme	Novel	0:4	arg1	system					25:30	Novel hydrophilic matrix system	0:30	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics	0:97	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics.					
30171979	1	29	theme	distance	273:280	arg1	increase					257:264	progressive increase	245:264	progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front	245:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	3	30	theme	latter	731:736	arg1	concentrations					709:722	decreasing concentrations	698:722	decreasing concentrations of the latter	698:736	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	1	31	theme	hydrophilic	184:194	arg1	systems					203:209	hydrophilic matrix systems	184:209	hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front	184:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	4	32	theme	resulting	798:806	arg1	matrices					817:824	The resulting gradient matrices	794:824	The resulting gradient matrices	794:824	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	1	33	theme	matrix	196:201	arg1	systems					203:209	hydrophilic matrix systems	184:209	hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front	184:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	0	34	theme	matrix	18:23	arg1	system					25:30	Novel hydrophilic matrix system	0:30	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics	0:97	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics.					
30171979	1	35	theme	drug	286:289	arg1	molecules					291:299	the drug molecules	282:299	the drug molecules	282:299	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	2	36	theme	ingredient	512:521	arg1	distribution					485:496	non-uniform distribution	473:496	non-uniform distribution of the active ingredient throughout the swellable polymer matrix	473:561	In order to solve this issue, a novel formulation approach based on non-uniform distribution of the active ingredient throughout the swellable polymer matrix was proposed and evaluated.					
30171979	7	37	theme	polymeric	1331:1339	arg1	material					1341:1348	the polymeric material	1327:1348	the polymeric material of established use	1327:1367	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	0	38	theme	non-uniform	37:47	arg1	distribution					54:65	non-uniform drug distribution	37:65	non-uniform drug distribution	37:65	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics.					
30171979	3	39	theme	high-viscosity	629:642	arg1	polymer					620:626	polymer	620:626	polymer (high-viscosity hypromellose)	620:656	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	3	39	theme	high-viscosity	629:642	arg1	hypromellose					644:655	high-viscosity hypromellose	629:655	high-viscosity hypromellose	629:655	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	2	40	theme	active	505:510	arg1	ingredient					512:521	the active ingredient	501:521	the active ingredient throughout the swellable polymer matrix	501:561	In order to solve this issue, a novel formulation approach based on non-uniform distribution of the active ingredient throughout the swellable polymer matrix was proposed and evaluated.					
30171979	1	41	theme	glassy	368:373	arg1	matrix					375:380	the glassy matrix	364:380	the glassy matrix	364:380	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	5	42	theme	Raman	1038:1042	arg1	analysis					1052:1059	Raman mapping analysis	1038:1059	Raman mapping analysis	1038:1059	The non-uniform matrix composition pursued was confirmed by Raman mapping analysis.					
30171979	7	43	theme	matrix	1474:1479	arg1	systems					1481:1487	hydrophilic matrix systems	1462:1487	hydrophilic matrix systems	1462:1487	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	1	44	from	reduction	339:347	arg1	front					398:402	the swelling front	385:402	the swelling front	385:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	1	45	theme	matrix	375:380	arg1	area					356:359	the area	352:359	the area of the glassy matrix at the swelling front	352:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	5	46	theme	mapping	1044:1050	arg1	analysis					1052:1059	Raman mapping analysis	1038:1059	Raman mapping analysis	1038:1059	The non-uniform matrix composition pursued was confirmed by Raman mapping analysis.					
30171979	0	47	with	system	25:30	arg1	distribution					54:65	non-uniform drug distribution	37:65	non-uniform drug distribution	37:65	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics.					
30171979	1	48	theme	oral	215:218	arg1	release					230:236	oral prolonged release	215:236	oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front	215:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	7	49	theme	design	1221:1226	arg1	concept					1228:1234	The simple design concept	1210:1234	The simple design concept	1210:1234	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	7	49	theme	design	1221:1226	arg1	technique					1250:1258	advantageous technique	1237:1258	advantageous technique	1237:1258	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	4	50	theme	spherical	901:909	arg1	shape					911:915	spherical shape	901:915	spherical shape	901:915	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	3	51	theme	polymer	620:626	arg1	mixtures					608:615	Various physical mixtures	591:615	Various physical mixtures	591:615	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	4	52	theme	gradient	808:815	arg1	matrices					817:824	The resulting gradient matrices	794:824	The resulting gradient matrices	794:824	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	7	53	theme	systems	1481:1487	arg1	performance					1447:1457	the performance	1443:1457	the performance of hydrophilic matrix systems	1443:1487	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	3	54	contain	having	691:696	arg2	concentrations					709:722	decreasing concentrations	698:722	decreasing concentrations of the latter	698:736	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	3	54	contain	having	691:696	arg1	mixtures					608:615	Various physical mixtures	591:615	Various physical mixtures	591:615	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	0	55	theme	drug	49:52	arg1	distribution					54:65	non-uniform drug distribution	37:65	non-uniform drug distribution	37:65	Novel hydrophilic matrix system with non-uniform drug distribution for zero-order release kinetics.					
30171979	2	56	theme	polymer	548:554	arg1	matrix					556:561	the swellable polymer matrix	534:561	the swellable polymer matrix	534:561	In order to solve this issue, a novel formulation approach based on non-uniform distribution of the active ingredient throughout the swellable polymer matrix was proposed and evaluated.					
30171979	3	57	theme	drug	662:665	arg1	acetaminophen					675:687	drug tracer (acetaminophen)	662:688	drug tracer (acetaminophen)	662:688	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	7	58	theme	simple	1214:1219	arg1	concept					1228:1234	The simple design concept	1210:1234	The simple design concept	1210:1234	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	7	58	theme	simple	1214:1219	arg1	technique					1250:1258	advantageous technique	1237:1258	advantageous technique	1237:1258	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	1	59	theme	release	118:124	arg1	rate					126:129	the release rate	114:129	the release rate over time	114:139	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	1	60	from	decrease	102:109	arg1	rate					126:129	the release rate	114:129	the release rate over time	114:139	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	2	61	theme	swellable	538:546	arg1	matrix					556:561	the swellable polymer matrix	534:561	the swellable polymer matrix	534:561	In order to solve this issue, a novel formulation approach based on non-uniform distribution of the active ingredient throughout the swellable polymer matrix was proposed and evaluated.					
30171979	1	62	theme	swelling	389:396	arg1	front					398:402	the swelling front	385:402	the swelling front	385:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	3	63	theme	physical	599:606	arg1	mixtures					608:615	Various physical mixtures	591:615	Various physical mixtures	591:615	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	7	64	theme	valuable	1414:1421	arg1	strategy					1423:1430	a valuable strategy	1412:1430	a valuable strategy to improve the performance of hydrophilic matrix systems	1412:1487	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	4	65	contain	possess	836:842	arg1	matrices					817:824	The resulting gradient matrices	794:824	The resulting gradient matrices	794:824	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	4	65	contain	possess	836:842	arg2	characteristics					879:893	satisfactory physico-technological characteristics	844:893	satisfactory physico-technological characteristics	844:893	The resulting gradient matrices showed to possess satisfactory physico-technological characteristics, with spherical shape and consistent thickness of the layers sequentially applied.					
30171979	1	66	theme	prolonged	220:228	arg1	release					230:236	oral prolonged release	215:236	oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front	215:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	7	67	theme	delivery	1378:1385	arg1	platform					1387:1394	the delivery platform	1374:1394	the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems	1374:1487	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	6	68	theme	drug	1123:1126	arg1	tracer					1128:1133	the drug tracer	1119:1133	the drug tracer	1119:1133	As compared with a system having uniform distribution of the drug tracer, the multi-layer formulations were proved to enhance linearity of release.					
30171979	1	69	from	front	398:402	arg1	reduction					339:347	reduction	339:347	reduction	339:347	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	1	69	from	front	398:402	arg1	area					356:359	the area	352:359	the area of the glassy matrix at the swelling front	352:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	1	69	from	front	398:402	arg1	outwards					326:333	outwards	326:333	outwards	326:333	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	6	70	theme	tracer	1128:1133	arg1	distribution					1103:1114	uniform distribution	1095:1114	uniform distribution of the drug tracer	1095:1133	As compared with a system having uniform distribution of the drug tracer, the multi-layer formulations were proved to enhance linearity of release.					
30171979	7	71	theme	use	1365:1367	arg1	concept					1228:1234	The simple design concept	1210:1234	The simple design concept	1210:1234	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	7	71	theme	use	1365:1367	arg1	technique					1250:1258	advantageous technique	1237:1258	advantageous technique	1237:1258	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	7	71	theme	use	1365:1367	arg1	material					1341:1348	the polymeric material	1327:1348	the polymeric material of established use	1327:1367	The simple design concept, advantageous technique, which involves no solvents nor high-impact drying operations, and the polymeric material of established use make the delivery platform hereby proposed a valuable strategy to improve the performance of hydrophilic matrix systems.					
30171979	1	72	theme	due	238:240	arg1	release					230:236	oral prolonged release	215:236	oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front	215:402	A decrease in the release rate over time is typically encountered when dealing with hydrophilic matrix systems for oral prolonged release due to progressive increase of the distance the drug molecules have to cover to diffuse outwards and reduction of the area of the glassy matrix at the swelling front.					
30171979	3	73	theme	acetaminophen	675:687	arg1	mixtures					608:615	Various physical mixtures	591:615	Various physical mixtures	591:615	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	3	74	theme	Various	591:597	arg1	mixtures					608:615	Various physical mixtures	591:615	Various physical mixtures	591:615	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	3	75	theme	core	782:785	arg1	seeds					787:791	inert core seeds	776:791	inert core seeds	776:791	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
30171979	3	76	theme	inert	776:780	arg1	seeds					787:791	inert core seeds	776:791	inert core seeds	776:791	Various physical mixtures of polymer (high-viscosity hypromellose) and drug tracer (acetaminophen), having decreasing concentrations of the latter, were applied by powder-layering onto inert core seeds.					
31378498	4	0	theme	total	752:756	arg1	ration					764:769	A total mixed ration	750:769	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis)	750:856	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	0	1	theme	protein	138:144	arg1	supply					146:151	the same metabolizable protein supply	115:151	the same metabolizable protein supply	115:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	18	2	theme	total	2935:2939	arg1	amount					2941:2946	the total amount	2931:2946	the total amount of EAA infused	2931:2961	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	18	2	theme	total	2935:2939	arg1	infused					2955:2961	EAA infused	2951:2961	EAA infused	2951:2961	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	6	3	theme	complete	1201:1208	arg1	mixture					1231:1237	a complete essential amino acid mixture	1199:1237	a complete essential amino acid mixture (EAAC)	1199:1244	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	3	theme	complete	1201:1208	arg1	profiles					1181:1188	The profiles	1177:1188	The profiles	1177:1188	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	3	theme	complete	1201:1208	arg1	EAAC					1240:1243	EAAC	1240:1243	EAAC	1240:1243	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	1	4	theme	cattle	229:234	arg1	diets					236:240	dairy cattle diets	223:240	dairy cattle diets	223:240	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	19	5	theme	milk	3169:3172	arg1	efficiency					3176:3185	milk N efficiency	3169:3185	milk N efficiency	3169:3185	Infusion of only Ile, Leu, and Val decreased milk protein yield and content and reduced milk N efficiency compared with a complete EAA profile.					
31378498	19	6	theme	complete	3203:3210	arg1	profile					3216:3222	a complete EAA profile	3201:3222	a complete EAA profile	3201:3222	Infusion of only Ile, Leu, and Val decreased milk protein yield and content and reduced milk N efficiency compared with a complete EAA profile.					
31378498	3	7	theme	square	628:633	arg1	design					635:640	a 5 × 5 Latin square design	614:640	a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest	614:747	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	14	8	theme	protein	2403:2409	arg1	yields					2424:2429	milk, protein, and lactose yields	2397:2429	milk, protein, and lactose yields	2397:2429	Compared with EAAC, infusion of ILV decreased or tended to decrease milk, protein, and lactose yields and milk protein content, and increased milk fat and lactose content.					
31378498	18	9	theme	EAA	2951:2953	arg1	infused					2955:2961	EAA infused	2951:2961	EAA infused	2951:2961	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	3	10	theme	dairy	508:512	arg1	cows					514:517	Five rumen-fistulated Holstein-Friesian dairy cows	468:517	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation)	468:586	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	1	11	theme	acid	160:163	arg1	composition					165:175	Amino acid composition	154:175	Amino acid composition of metabolizable protein (MP)	154:205	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	10	12	theme	milk	1927:1930	arg1	fat					1932:1934	milk fat	1927:1934	milk fat	1927:1934	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	1	13	from	effects	247:253	arg1	energy					275:280	energy	275:280	energy	275:280	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	1	13	from	effects	247:253	arg1	utilization					288:298	N utilization	286:298	N utilization	286:298	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	8	14	theme	milk	1708:1711	arg1	efficiency					1715:1724	milk N efficiency	1708:1724	milk N efficiency	1708:1724	Compared with SAL, infusion of EAAC increased milk, protein, and lactose yield, increased energy retained as body protein, and did not affect milk N efficiency.					
31378498	10	15	theme	lactose	1950:1956	arg1	yield					1906:1910	the same yield	1897:1910	the same yield	1897:1910	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	10	15	theme	lactose	1950:1956	arg1	yield					1887:1891	the same milk yield	1873:1891	the same milk yield	1873:1891	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	10	15	theme	lactose	1950:1956	arg1	content					1916:1922	content	1916:1922	content	1916:1922	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	0	16	theme	profiles	103:110	arg1	provision					59:67	provision	59:67	provision of different essential amino acid profiles at the same metabolizable protein supply	59:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	14	17	theme	protein	2440:2446	arg1	content					2448:2454	milk protein content	2435:2454	milk protein content	2435:2454	Compared with EAAC, infusion of ILV decreased or tended to decrease milk, protein, and lactose yields and milk protein content, and increased milk fat and lactose content.					
31378498	1	18	theme	protein	194:200	arg1	composition					165:175	Amino acid composition	154:175	Amino acid composition of metabolizable protein (MP)	154:205	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	6	19	dep	Phe	1300:1302	arg1	Met					1295:1297	His, Ile, Leu, Met, Phe	1280:1302	Met	1295:1297	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	19	dep	Phe	1300:1302	arg1	Leu					1290:1292	His, Ile, Leu, Met, Phe	1280:1302	Leu	1290:1292	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	19	dep	Phe	1300:1302	arg1	Ile					1285:1287	His, Ile, Leu, Met, Phe	1280:1302	Ile	1285:1287	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	4	20	theme	16	802:803	arg1	%					804:804	%	804:804	%	804:804	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	0	21	from	provision	59:67	arg1	supply					146:151	the same metabolizable protein supply	115:151	the same metabolizable protein supply	115:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	12	22	theme	matter	2206:2211	arg1	intake					2213:2218	dry matter intake	2202:2218	dry matter intake	2202:2218	Infusion of ILV tended to decrease dry matter intake compared with the other AA infusions.					
31378498	7	23	from	balance	1465:1471	arg1	conjunction					1476:1486	conjunction	1476:1486	conjunction with milk production and composition, digestibility, and plasma constituents	1476:1563	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	19	24	theme	Leu	3103:3105	arg1	Infusion					3081:3088	Infusion	3081:3088	Infusion of only Ile, Leu, and Val	3081:3114	Infusion of only Ile, Leu, and Val decreased milk protein yield and content and reduced milk N efficiency compared with a complete EAA profile.					
31378498	10	25	theme	similar	1967:1973	arg1	energy					1975:1980	similar energy	1967:1980	similar energy	1967:1980	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	0	26	theme	metabolizable	124:136	arg1	supply					146:151	the same metabolizable protein supply	115:151	the same metabolizable protein supply	115:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	10	27	theme	same	1901:1904	arg1	yield					1906:1910	the same yield	1897:1910	the same yield	1897:1910	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	16	28	theme	Milk	2576:2579	arg1	concentration					2586:2598	Milk urea concentration	2576:2598	Milk urea concentration	2576:2598	Milk urea concentration was not affected by essential amino acid (EAA) infusions.					
31378498	3	29	theme	rest	744:747	arg1	d					739:739	2 d	737:739	2 d of rest	737:747	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	4	30	theme	%	787:787	arg1	silage					794:799	58% corn silage	785:799	58% corn silage	785:799	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	4	31	theme	libitum	967:973	arg1	intake					975:980	ad libitum intake	964:980	ad libitum intake	964:980	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	3	32	theme	continuous	696:705	arg1	infusion					716:723	continuous abomasal infusion	696:723	continuous abomasal infusion	696:723	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	19	33	theme	Ile	3098:3100	arg1	Infusion					3081:3088	Infusion	3081:3088	Infusion of only Ile, Leu, and Val	3081:3114	Infusion of only Ile, Leu, and Val decreased milk protein yield and content and reduced milk N efficiency compared with a complete EAA profile.					
31378498	4	34	theme	26	823:824	arg1	%					825:825	%	825:825	%	825:825	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	18	35	theme	EAA	2914:2916	arg1	profile					2918:2924	a complete EAA profile	2903:2924	a complete EAA profile when the total amount of EAA infused remained constant	2903:2979	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	17	36	theme	urea	2665:2668	arg1	concentration					2670:2682	Plasma urea concentration	2658:2682	Plasma urea concentration	2658:2682	Plasma urea concentration did not differ between EAAC and SAL, tended to increase with ILV and GR1+ILV over SAL, and increased with GR1+ALT compared with EAAC and SAL.					
31378498	18	37	theme	N	3022:3022	arg1	efficiency					3024:3033	milk N efficiency	3017:3033	milk N efficiency	3017:3033	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	1	38	theme	N	286:286	arg1	utilization					288:298	N utilization	286:298	N utilization	286:298	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	10	39	theme	milk	1882:1885	arg1	yield					1887:1891	the same milk yield	1873:1891	the same milk yield	1873:1891	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	15	40	theme	N	2506:2506	arg1	efficiency					2508:2517	Milk N efficiency	2501:2517	Milk N efficiency	2501:2517	Milk N efficiency decreased with ILV compared with SAL, EAAC, and GR1+ILV.					
31378498	9	41	theme	N	1733:1733	arg1	intake					1735:1740	Total N intake	1727:1740	Total N intake	1727:1740	Total N intake and urine N output was higher with all AA infusions relative to SAL.					
31378498	3	42	dep	lactations	530:539	arg1	deviation					577:585	mean ± standard deviation	561:585	2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation	520:585	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	3	42	dep	lactations	530:539	arg1	d					550:550	81 ± 11 d	542:550	2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation	520:585	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	4	43	theme	alfalfa	806:812	arg1	hay					814:816	16% alfalfa hay	802:816	16% alfalfa hay	802:816	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	16	44	theme	essential	2620:2628	arg1	infusions					2647:2655	essential amino acid (EAA) infusions	2620:2655	essential amino acid (EAA) infusions	2620:2655	Milk urea concentration was not affected by essential amino acid (EAA) infusions.					
31378498	3	45	theme	infusion	716:723	arg1	d					691:691	5 d	689:691	5 d of continuous abomasal infusion	689:723	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	0	46	theme	different	72:80	arg1	profiles					103:110	different essential amino acid profiles	72:110	different essential amino acid profiles at the same metabolizable protein supply	72:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	16	47	theme	acid	2636:2639	arg1	infusions					2647:2655	essential amino acid (EAA) infusions	2620:2655	essential amino acid (EAA) infusions	2620:2655	Milk urea concentration was not affected by essential amino acid (EAA) infusions.					
31378498	19	48	theme	protein	3131:3137	arg1	yield					3139:3143	milk protein yield	3126:3143	milk protein yield	3126:3143	Infusion of only Ile, Leu, and Val decreased milk protein yield and content and reduced milk N efficiency compared with a complete EAA profile.					
31378498	2	49	theme	different	349:357	arg1	profiles					362:369	different AA profiles	349:369	different AA profiles within a constant supplemental MP level	349:409	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	0	50	theme	amino	92:96	arg1	profiles					103:110	different essential amino acid profiles	72:110	different essential amino acid profiles at the same metabolizable protein supply	72:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	12	51	theme	AA	2244:2245	arg1	infusions					2247:2255	the other AA infusions	2234:2255	the other AA infusions	2234:2255	Infusion of ILV tended to decrease dry matter intake compared with the other AA infusions.					
31378498	16	52	theme	EAA	2642:2644	arg1	infusions					2647:2655	essential amino acid (EAA) infusions	2620:2655	essential amino acid (EAA) infusions	2620:2655	Milk urea concentration was not affected by essential amino acid (EAA) infusions.					
31378498	2	53	theme	dairy	454:458	arg1	cattle					460:465	dairy cattle	454:465	dairy cattle	454:465	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	5	54	theme	individual	1105:1114	arg1	content					1119:1125	individual AA content	1105:1125	individual AA content	1105:1125	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	0	55	theme	Energy	0:5	arg1	balance					20:26	Energy and nitrogen balance	0:26	balance	20:26	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	8	56	theme	EAAC	1597:1600	arg1	infusion					1585:1592	infusion	1585:1592	infusion of EAAC	1585:1600	Compared with SAL, infusion of EAAC increased milk, protein, and lactose yield, increased energy retained as body protein, and did not affect milk N efficiency.					
31378498	2	57	theme	supplemental	389:400	arg1	level					405:409	a constant supplemental MP level	378:409	a constant supplemental MP level	378:409	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	5	58	theme	AA	1072:1073	arg1	SAL					1043:1045	SAL	1043:1045	SAL	1043:1045	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	5	58	theme	AA	1072:1073	arg1	saline					1035:1040	saline	1035:1040	saline (SAL)	1035:1046	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	5	58	theme	AA	1072:1073	arg1	g/d					1055:1057	562 g/d	1051:1057	562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein	1051:1174	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	0	59	theme	nitrogen	11:18	arg1	balance					20:26	Energy and nitrogen balance	0:26	balance	20:26	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	8	60	theme	milk	1612:1615	arg1	protein					1618:1624	milk, protein, and lactose yield	1612:1643	protein	1618:1624	Compared with SAL, infusion of EAAC increased milk, protein, and lactose yield, increased energy retained as body protein, and did not affect milk N efficiency.					
31378498	4	61	theme	dry	840:842	arg1	basis					851:855	dry matter basis	840:855	dry matter basis	840:855	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	4	61	theme	dry	840:842	arg1	concentrate					827:837	26% concentrate	823:837	26% concentrate (dry matter basis)	823:856	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	9	62	theme	AA	1781:1782	arg1	infusions					1784:1792	all AA infusions	1777:1792	all AA infusions relative to SAL	1777:1808	Total N intake and urine N output was higher with all AA infusions relative to SAL.					
31378498	2	63	theme	profiles	362:369	arg1	effect					339:344	the effect	335:344	the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning	335:449	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	14	64	theme	ILV	2361:2363	arg1	infusion					2349:2356	infusion	2349:2356	infusion of ILV	2349:2363	Compared with EAAC, infusion of ILV decreased or tended to decrease milk, protein, and lactose yields and milk protein content, and increased milk fat and lactose content.					
31378498	1	65	theme	imbalances	261:270	arg1	effects					247:253	effects	247:253	effects of AA imbalances on energy and N utilization	247:298	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	7	66	theme	climate	1410:1416	arg1	chambers					1430:1437	climate respiration chambers	1410:1437	climate respiration chambers	1410:1437	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	2	67	from	effect	339:344	arg1	energy					425:430	whole-body energy	414:430	whole-body energy	414:430	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	2	67	from	effect	339:344	arg1	partitioning					438:449	N partitioning	436:449	N partitioning	436:449	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	2	68	theme	whole-body	414:423	arg1	energy					425:430	whole-body energy	414:430	whole-body energy	414:430	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	3	69	theme	±	566:566	arg1	deviation					577:585	mean ± standard deviation	561:585	2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation	520:585	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	5	70	theme	relative	1149:1156	arg1	content					1158:1164	their relative content	1143:1164	their relative content in casein	1143:1174	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	9	71	theme	relative	1794:1801	arg1	infusions					1784:1792	all AA infusions	1777:1792	all AA infusions relative to SAL	1777:1808	Total N intake and urine N output was higher with all AA infusions relative to SAL.					
31378498	0	72	theme	cattle	37:42	arg1	balance					20:26	Energy and nitrogen balance	0:26	balance	20:26	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	5	73	theme	Abomasal	1001:1008	arg1	treatments					1019:1028	Abomasal infusion treatments	1001:1028	Abomasal infusion treatments	1001:1028	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	6	74	dep	mixture	1231:1237	arg1	Phe					1300:1302	Phe	1300:1302	Phe	1300:1302	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	74	dep	mixture	1231:1237	arg1	3					1277:1277	3	1277:1277	3	1277:1277	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	74	dep	mixture	1231:1237	arg1	1					1196:1196	1	1196:1196	1	1196:1196	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	4	75	theme	energy	900:905	arg1	requirements					914:925	MP requirements	911:925	MP requirements	911:925	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	4	75	theme	energy	900:905	arg1	%					891:891	100 and 83%	881:891	100 and 83% of net energy	881:905	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	4	75	theme	energy	900:905	arg1	energy					900:905	net energy	896:905	net energy	896:905	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	18	76	theme	milk	2996:2999	arg1	production					3001:3010	milk production	2996:3010	milk production	2996:3010	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	6	77	theme	acid	1226:1229	arg1	mixture					1231:1237	a complete essential amino acid mixture	1199:1237	a complete essential amino acid mixture (EAAC)	1199:1244	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	77	theme	acid	1226:1229	arg1	profiles					1181:1188	The profiles	1177:1188	The profiles	1177:1188	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	77	theme	acid	1226:1229	arg1	EAAC					1240:1243	EAAC	1240:1243	EAAC	1240:1243	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	14	78	theme	milk	2397:2400	arg1	yields					2424:2429	milk, protein, and lactose yields	2397:2429	milk, protein, and lactose yields	2397:2429	Compared with EAAC, infusion of ILV decreased or tended to decrease milk, protein, and lactose yields and milk protein content, and increased milk fat and lactose content.					
31378498	19	79	theme	EAA	3212:3214	arg1	profile					3216:3222	a complete EAA profile	3201:3222	a complete EAA profile	3201:3222	Infusion of only Ile, Leu, and Val decreased milk protein yield and content and reduced milk N efficiency compared with a complete EAA profile.					
31378498	11	80	theme	Milk	1999:2002	arg1	efficiency					2006:2015	Milk N efficiency	1999:2015	Milk N efficiency	1999:2015	Milk N efficiency was not different between EAAC and GR1+ILV, but was lower with GR1+ALT compared with EAAC, and tended to be lower with GR1+ALT compared with GR1+ILV.					
31378498	3	81	theme	×	618:618	arg1	design					635:640	a 5 × 5 Latin square design	614:640	a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest	614:747	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	6	82	theme	essential	1210:1218	arg1	mixture					1231:1237	a complete essential amino acid mixture	1199:1237	a complete essential amino acid mixture (EAAC)	1199:1244	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	82	theme	essential	1210:1218	arg1	profiles					1181:1188	The profiles	1177:1188	The profiles	1177:1188	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	82	theme	essential	1210:1218	arg1	EAAC					1240:1243	EAAC	1240:1243	EAAC	1240:1243	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	1	83	theme	dairy	223:227	arg1	diets					236:240	dairy cattle diets	223:240	dairy cattle diets	223:240	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	0	84	from	supply	146:151	arg1	provision					59:67	provision	59:67	provision of different essential amino acid profiles at the same metabolizable protein supply	59:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	0	84	from	supply	146:151	arg1	profiles					103:110	different essential amino acid profiles	72:110	different essential amino acid profiles at the same metabolizable protein supply	72:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	4	85	theme	%	825:825	arg1	basis					851:855	dry matter basis	840:855	dry matter basis	840:855	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	4	85	theme	%	825:825	arg1	concentrate					827:837	26% concentrate	823:837	26% concentrate (dry matter basis)	823:856	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	13	86	theme	Milk	2258:2261	arg1	production					2263:2272	Milk production	2258:2272	Milk production	2258:2272	Milk production and composition was not different between ILV and SAL.					
31378498	4	87	theme	mixed	758:762	arg1	ration					764:769	A total mixed ration	750:769	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis)	750:856	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	8	88	theme	body	1675:1678	arg1	protein					1680:1686	body protein	1675:1686	body protein	1675:1686	Compared with SAL, infusion of EAAC increased milk, protein, and lactose yield, increased energy retained as body protein, and did not affect milk N efficiency.					
31378498	18	89	theme	infused	2955:2961	arg1	amount					2941:2946	the total amount	2931:2946	the total amount of EAA infused	2931:2961	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	18	89	theme	infused	2955:2961	arg1	infused					2955:2961	EAA infused	2951:2961	EAA infused	2951:2961	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	3	90	theme	Holstein-Friesian	490:506	arg1	cows					514:517	Five rumen-fistulated Holstein-Friesian dairy cows	468:517	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation)	468:586	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	14	91	theme	milk	2471:2474	arg1	fat					2476:2478	milk fat	2471:2478	milk fat	2471:2478	Compared with EAAC, infusion of ILV decreased or tended to decrease milk, protein, and lactose yields and milk protein content, and increased milk fat and lactose content.					
31378498	1	92	theme	Amino	154:158	arg1	composition					165:175	Amino acid composition	154:175	Amino acid composition of metabolizable protein (MP)	154:205	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	7	93	theme	plasma	1545:1550	arg1	constituents					1552:1563	plasma constituents	1545:1563	plasma constituents	1545:1563	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	6	94	dep	Ile	1251:1253	arg1	2					1248:1248	2	1248:1248	2	1248:1248	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	10	95	theme	fat	1932:1934	arg1	yield					1906:1910	the same yield	1897:1910	the same yield	1897:1910	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	10	95	theme	fat	1932:1934	arg1	yield					1887:1891	the same milk yield	1873:1891	the same milk yield	1873:1891	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	10	95	theme	fat	1932:1934	arg1	content					1916:1922	content	1916:1922	content	1916:1922	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	3	96	theme	Latin	622:626	arg1	design					635:640	a 5 × 5 Latin square design	614:640	a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest	614:747	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	4	97	theme	MP	911:912	arg1	requirements					914:925	MP requirements	911:925	MP requirements	911:925	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	3	98	theme	experimental	656:667	arg1	period					669:674	each experimental period	651:674	each experimental period	651:674	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	1	99	from	diets	236:240	arg1	important					210:218	important	210:218	important	210:218	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	1	100	theme	metabolizable	180:192	arg1	protein					194:200	metabolizable protein	180:200	metabolizable protein (MP)	180:205	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	1	100	theme	metabolizable	180:192	arg1	MP					203:204	MP	203:204	MP	203:204	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	12	101	theme	other	2238:2242	arg1	infusions					2247:2255	the other AA infusions	2234:2255	the other AA infusions	2234:2255	Infusion of ILV tended to decrease dry matter intake compared with the other AA infusions.					
31378498	4	102	theme	corn	789:792	arg1	silage					794:799	58% corn silage	785:799	58% corn silage	785:799	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	12	103	theme	dry	2202:2204	arg1	intake					2213:2218	dry matter intake	2202:2218	dry matter intake	2202:2218	Infusion of ILV tended to decrease dry matter intake compared with the other AA infusions.					
31378498	0	104	theme	same	119:122	arg1	supply					146:151	the same metabolizable protein supply	115:151	the same metabolizable protein supply	115:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	3	105	theme	±	545:545	arg1	d					550:550	81 ± 11 d	542:550	2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation	520:585	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	14	106	theme	milk	2435:2438	arg1	content					2448:2454	milk protein content	2435:2454	milk protein content	2435:2454	Compared with EAAC, infusion of ILV decreased or tended to decrease milk, protein, and lactose yields and milk protein content, and increased milk fat and lactose content.					
31378498	8	107	theme	N	1713:1713	arg1	efficiency					1715:1724	milk N efficiency	1708:1724	milk N efficiency	1708:1724	Compared with SAL, infusion of EAAC increased milk, protein, and lactose yield, increased energy retained as body protein, and did not affect milk N efficiency.					
31378498	4	108	theme	ad	964:965	arg1	intake					975:980	ad libitum intake	964:980	ad libitum intake	964:980	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	10	109	theme	protein	1937:1943	arg1	yield					1906:1910	the same yield	1897:1910	the same yield	1897:1910	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	10	109	theme	protein	1937:1943	arg1	yield					1887:1891	the same milk yield	1873:1891	the same milk yield	1873:1891	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	10	109	theme	protein	1937:1943	arg1	content					1916:1922	content	1916:1922	content	1916:1922	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	10	110	theme	same	1877:1880	arg1	yield					1887:1891	the same milk yield	1873:1891	the same milk yield	1873:1891	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	1	111	from	important	210:218	arg1	diets					236:240	dairy cattle diets	223:240	dairy cattle diets	223:240	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	7	112	theme	milk	1493:1496	arg1	production					1498:1507	milk production	1493:1507	milk production	1493:1507	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	4	113	theme	intake	975:980	arg1	%					959:959	90%	957:959	90% of ad libitum intake	957:980	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	4	113	theme	intake	975:980	arg1	intake					975:980	ad libitum intake	964:980	ad libitum intake	964:980	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	18	114	theme	milk	3017:3020	arg1	efficiency					3024:3033	milk N efficiency	3017:3033	milk N efficiency	3017:3033	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	3	115	dep	cows	514:517	arg1	lactations					530:539	2.8 ± 0.4 lactations	520:539	2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation	520:585	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	4	116	theme	58	785:786	arg1	%					787:787	%	787:787	%	787:787	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	19	117	theme	Val	3112:3114	arg1	Infusion					3081:3088	Infusion	3081:3088	Infusion of only Ile, Leu, and Val	3081:3114	Infusion of only Ile, Leu, and Val decreased milk protein yield and content and reduced milk N efficiency compared with a complete EAA profile.					
31378498	17	118	theme	Plasma	2658:2663	arg1	concentration					2670:2682	Plasma urea concentration	2658:2682	Plasma urea concentration	2658:2682	Plasma urea concentration did not differ between EAAC and SAL, tended to increase with ILV and GR1+ILV over SAL, and increased with GR1+ALT compared with EAAC and SAL.					
31378498	3	119	theme	abomasal	707:714	arg1	infusion					716:723	continuous abomasal infusion	696:723	continuous abomasal infusion	696:723	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	16	120	theme	urea	2581:2584	arg1	concentration					2586:2598	Milk urea concentration	2576:2598	Milk urea concentration	2576:2598	Milk urea concentration was not affected by essential amino acid (EAA) infusions.					
31378498	15	121	theme	Milk	2501:2504	arg1	efficiency					2508:2517	Milk N efficiency	2501:2517	Milk N efficiency	2501:2517	Milk N efficiency decreased with ILV compared with SAL, EAAC, and GR1+ILV.					
31378498	10	122	theme	GR1+ILV	1844:1850	arg1	infusions					1831:1839	infusions	1831:1839	infusions of GR1+ILV and GR1+ALT	1831:1862	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	18	123	theme	complete	2905:2912	arg1	profile					2918:2924	a complete EAA profile	2903:2924	a complete EAA profile when the total amount of EAA infused remained constant	2903:2979	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	10	124	theme	N	1986:1986	arg1	retention					1988:1996	N retention	1986:1996	N retention	1986:1996	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	14	125	theme	lactose	2484:2490	arg1	content					2492:2498	lactose content	2484:2498	lactose content	2484:2498	Compared with EAAC, infusion of ILV decreased or tended to decrease milk, protein, and lactose yields and milk protein content, and increased milk fat and lactose content.					
31378498	9	126	theme	Total	1727:1731	arg1	intake					1735:1740	Total N intake	1727:1740	Total N intake	1727:1740	Total N intake and urine N output was higher with all AA infusions relative to SAL.					
31378498	14	127	theme	lactose	2416:2422	arg1	yields					2424:2429	milk, protein, and lactose yields	2397:2429	milk, protein, and lactose yields	2397:2429	Compared with EAAC, infusion of ILV decreased or tended to decrease milk, protein, and lactose yields and milk protein content, and increased milk fat and lactose content.					
31378498	2	128	theme	N	436:436	arg1	partitioning					438:449	N partitioning	436:449	N partitioning	436:449	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	16	129	theme	amino	2630:2634	arg1	infusions					2647:2655	essential amino acid (EAA) infusions	2620:2655	essential amino acid (EAA) infusions	2620:2655	Milk urea concentration was not affected by essential amino acid (EAA) infusions.					
31378498	7	130	with	conjunction	1476:1486	arg1	production					1498:1507	milk production	1493:1507	milk production	1493:1507	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	7	130	with	conjunction	1476:1486	arg1	constituents					1552:1563	plasma constituents	1545:1563	plasma constituents	1545:1563	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	7	130	with	conjunction	1476:1486	arg1	digestibility					1526:1538	digestibility	1526:1538	digestibility	1526:1538	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	7	130	with	conjunction	1476:1486	arg1	composition					1513:1523	composition	1513:1523	composition	1513:1523	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	0	131	theme	essential	82:90	arg1	profiles					103:110	different essential amino acid profiles	72:110	different essential amino acid profiles at the same metabolizable protein supply	72:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	7	132	theme	N	1463:1463	arg1	balance					1465:1471	energy and N balance	1452:1471	balance	1465:1471	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	6	133	dep	Arg	1333:1335	arg1	4					1330:1330	4	1330:1330	4	1330:1330	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	4	134	theme	%	804:804	arg1	hay					814:816	16% alfalfa hay	802:816	16% alfalfa hay	802:816	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	19	135	theme	milk	3126:3129	arg1	yield					3139:3143	milk protein yield	3126:3143	milk protein yield	3126:3143	Infusion of only Ile, Leu, and Val decreased milk protein yield and content and reduced milk N efficiency compared with a complete EAA profile.					
31378498	3	136	from	d	550:550	arg1	milk					555:558	milk	555:558	milk	555:558	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	9	137	theme	urine	1746:1750	arg1	output					1754:1759	urine N output	1746:1759	urine N output	1746:1759	Total N intake and urine N output was higher with all AA infusions relative to SAL.					
31378498	0	138	theme	acid	98:101	arg1	profiles					103:110	different essential amino acid profiles	72:110	different essential amino acid profiles at the same metabolizable protein supply	72:151	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	3	139	dep	consisted	676:684	arg1	followed					725:732	followed	725:732	followed by 2 d of rest	725:747	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	7	140	theme	energy	1452:1457	arg1	balance					1465:1471	energy and N balance	1452:1471	balance	1465:1471	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	19	141	theme	N	3174:3174	arg1	efficiency					3176:3185	milk N efficiency	3169:3185	milk N efficiency	3169:3185	Infusion of only Ile, Leu, and Val decreased milk protein yield and content and reduced milk N efficiency compared with a complete EAA profile.					
31378498	10	142	theme	GR1+ALT	1856:1862	arg1	infusions					1831:1839	infusions	1831:1839	infusions of GR1+ILV and GR1+ALT	1831:1862	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	4	143	theme	individual	985:994	arg1	cow					996:998	individual cow	985:998	individual cow	985:998	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	12	144	theme	ILV	2179:2181	arg1	Infusion					2167:2174	Infusion	2167:2174	Infusion of ILV	2167:2181	Infusion of ILV tended to decrease dry matter intake compared with the other AA infusions.					
31378498	2	145	theme	MP	402:403	arg1	level					405:409	a constant supplemental MP level	378:409	a constant supplemental MP level	378:409	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	5	146	theme	essential	1062:1070	arg1	AA					1072:1073	essential AA	1062:1073	essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein	1062:1174	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	7	147	theme	respiration	1418:1428	arg1	chambers					1430:1437	climate respiration chambers	1410:1437	climate respiration chambers	1410:1437	The experiment was conducted in climate respiration chambers to determine energy and N balance in conjunction with milk production and composition, digestibility, and plasma constituents.					
31378498	5	148	from	content	1158:1164	arg1	casein					1169:1174	casein	1169:1174	casein	1169:1174	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	2	149	theme	constant	380:387	arg1	level					405:409	a constant supplemental MP level	378:409	a constant supplemental MP level	378:409	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	3	150	theme	standard	568:575	arg1	deviation					577:585	mean ± standard deviation	561:585	2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation	520:585	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	0	151	theme	dairy	31:35	arg1	cattle					37:42	dairy cattle	31:42	dairy cattle	31:42	Energy and nitrogen balance of dairy cattle as affected by provision of different essential amino acid profiles at the same metabolizable protein supply.					
31378498	1	152	theme	AA	258:259	arg1	imbalances					261:270	AA imbalances	258:270	AA imbalances	258:270	Amino acid composition of metabolizable protein (MP) is important in dairy cattle diets, but effects of AA imbalances on energy and N utilization are unclear.					
31378498	10	153	contain	had	1963:1965	arg2	retention					1988:1996	N retention	1986:1996	N retention	1986:1996	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	10	153	contain	had	1963:1965	arg1	infusions					1831:1839	infusions	1831:1839	infusions of GR1+ILV and GR1+ALT	1831:1862	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	10	153	contain	had	1963:1965	arg2	energy					1975:1980	similar energy	1967:1980	similar energy	1967:1980	Compared with EAAC, infusions of GR1+ILV and GR1+ALT produced the same milk yield and the same yield and content of milk fat, protein, and lactose, and had similar energy and N retention.					
31378498	9	154	theme	N	1752:1752	arg1	output					1754:1759	urine N output	1746:1759	urine N output	1746:1759	Total N intake and urine N output was higher with all AA infusions relative to SAL.					
31378498	3	155	theme	mean	561:564	arg1	deviation					577:585	mean ± standard deviation	561:585	2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation	520:585	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	2	156	theme	AA	359:360	arg1	profiles					362:369	different AA profiles	349:369	different AA profiles within a constant supplemental MP level	349:409	This study determined the effect of different AA profiles within a constant supplemental MP level on whole-body energy and N partitioning in dairy cattle.					
31378498	18	157	from	profile	2918:2924	arg1	Val					2894:2896	Val	2894:2896	Val	2894:2896	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	18	157	from	profile	2918:2924	arg1	Ile					2880:2882	Ile	2880:2882	Ile	2880:2882	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	18	157	from	profile	2918:2924	arg1	Leu					2885:2887	Leu	2885:2887	Leu	2885:2887	In conclusion, removing Arg, Lys, and Thr or removing Ile, Leu, and Val from a complete EAA profile when the total amount of EAA infused remained constant did not impair milk production, but milk N efficiency decreased when Ile, Leu, and Val were absent.					
31378498	8	158	theme	lactose	1631:1637	arg1	yield					1639:1643	milk, protein, and lactose yield	1612:1643	yield	1639:1643	Compared with SAL, infusion of EAAC increased milk, protein, and lactose yield, increased energy retained as body protein, and did not affect milk N efficiency.					
31378498	4	159	theme	matter	844:849	arg1	basis					851:855	dry matter basis	840:855	dry matter basis	840:855	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	4	159	theme	matter	844:849	arg1	concentrate					827:837	26% concentrate	823:837	26% concentrate (dry matter basis)	823:856	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	11	160	theme	N	2004:2004	arg1	efficiency					2006:2015	Milk N efficiency	1999:2015	Milk N efficiency	1999:2015	Milk N efficiency was not different between EAAC and GR1+ILV, but was lower with GR1+ALT compared with EAAC, and tended to be lower with GR1+ALT compared with GR1+ILV.					
31378498	5	161	theme	AA	1116:1117	arg1	content					1119:1125	individual AA content	1105:1125	individual AA content	1105:1125	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	5	162	theme	infusion	1010:1017	arg1	treatments					1019:1028	Abomasal infusion treatments	1001:1028	Abomasal infusion treatments	1001:1028	Abomasal infusion treatments were saline (SAL) or 562 g/d of essential AA delivered in 4 profiles where individual AA content corresponded to their relative content in casein.					
31378498	4	163	theme	net	896:898	arg1	energy					900:905	net energy	896:905	net energy	896:905	A total mixed ration consisting of 58% corn silage, 16% alfalfa hay, and 26% concentrate (dry matter basis) was formulated to meet 100 and 83% of net energy and MP requirements, respectively, and was fed at 90% of ad libitum intake by individual cow.					
31378498	3	164	theme	rumen-fistulated	473:488	arg1	cows					514:517	Five rumen-fistulated Holstein-Friesian dairy cows	468:517	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation)	468:586	Five rumen-fistulated Holstein-Friesian dairy cows (2.8 ± 0.4 lactations; 81 ± 11 d in milk; mean ± standard deviation) were randomly assigned to a 5 × 5 Latin square design in which each experimental period consisted of 5 d of continuous abomasal infusion followed by 2 d of rest.					
31378498	6	165	theme	amino	1220:1224	arg1	mixture					1231:1237	a complete essential amino acid mixture	1199:1237	a complete essential amino acid mixture (EAAC)	1199:1244	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	165	theme	amino	1220:1224	arg1	profiles					1181:1188	The profiles	1177:1188	The profiles	1177:1188	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
31378498	6	165	theme	amino	1220:1224	arg1	EAAC					1240:1243	EAAC	1240:1243	EAAC	1240:1243	The profiles were (1) a complete essential amino acid mixture (EAAC), (2) Ile, Leu, and Val (ILV), (3) His, Ile, Leu, Met, Phe, Trp, Val (GR1+ILV), and (4) Arg, His, Lys, Met, Phe, Thr, Trp (GR1+ALT).					
30678897	3	0	theme	cell	522:525	arg1	method					535:540	a new cell seeding method	516:540	a new cell seeding method assuring 90% seeding efficiency on the scaffolds	516:589	Moreover, a new cell seeding method assuring 90% seeding efficiency on the scaffolds was developed.					
30678897	1	1	theme	scaffolds	271:279	arg1	performance					235:245	the biological performance	220:245	the biological performance of resorbable polymeric scaffolds for bone tissue engineering	220:307	The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering.					
30678897	4	2	theme	Poly	606:609	arg1	scaffolds					642:650	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds	606:650	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution	606:695	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	9	3	theme	bone	1626:1629	arg1	sialoprotein					1631:1642	bone sialoprotein II	1626:1645	bone sialoprotein II	1626:1645	However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II.					
30678897	3	4	theme	seeding	555:561	arg1	efficiency					563:572	90% seeding efficiency	551:572	90% seeding efficiency	551:572	Moreover, a new cell seeding method assuring 90% seeding efficiency on the scaffolds was developed.					
30678897	1	5	theme	bone	285:288	arg1	engineering					297:307	bone tissue engineering	285:307	bone tissue engineering	285:307	The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering.					
30678897	8	6	theme	bimodal	1304:1310	arg1	distribution					1317:1328	bimodal pore distribution	1304:1328	bimodal pore distribution	1304:1328	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	0	7	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells	0:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	4	8	theme	sulphated	874:882	arg1	sHya					896:899	sHya	896:899	sHya	896:899	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	4	8	theme	sulphated	874:882	arg1	hyaluronan					884:893	high sulphated hyaluronan	869:893	high sulphated hyaluronan (sHya)	869:900	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	4	9	theme	pore	679:682	arg1	distribution					684:695	bimodal pore distribution	671:695	bimodal pore distribution	671:695	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	8	10	attach	presence	1292:1299	arg1	combination					1333:1343	combination	1333:1343	combination with collagen/sHya	1333:1362	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	8	10	attach	presence	1292:1299	arg2	distribution					1317:1328	bimodal pore distribution	1304:1328	bimodal pore distribution	1304:1328	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	1	11	theme	study	199:203	arg1	objective					181:189	The main objective	172:189	The main objective of this study	172:203	The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering.					
30678897	8	12	dep	In	1265:1266	arg1	vitro					1268:1272	vitro	1268:1272	vitro	1268:1272	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	10	13	theme	bimodal	1680:1686	arg1	distribution					1693:1704	bimodal pore distribution	1680:1704	bimodal pore distribution	1680:1704	As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.					
30678897	0	14	theme	mesenchymal	148:158	arg1	cells					165:169	human mesenchymal stem cells	142:169	human mesenchymal stem cells	142:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	7	15	theme	homogenous	1188:1197	arg1	layer					1199:1203	a homogenous layer	1186:1203	a homogenous layer of aECM	1186:1211	The scaffolds were successfully coated with a homogenous layer of aECM as shown by Sirius red and toluidine blue staining.					
30678897	4	16	theme	casting/phase	722:734	arg1	separation					736:745	solvent casting/phase separation	714:745	solvent casting/phase separation followed by porogen leaching	714:774	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	0	17	theme	cells	165:169	arg1	differentiation					123:137	osteogenic differentiation	112:137	osteogenic differentiation of human mesenchymal stem cells	112:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	5	18	theme	porogens	926:933	arg1	application					907:917	The application	903:917	The application of two porogens	903:933	The application of two porogens resulted in bimodal pore distribution within the PLGA scaffolds as shown by scanning electron microscopy and microcomputer tomography.					
30678897	8	19	theme	distribution	1317:1328	arg1	presence					1292:1299	presence	1292:1299	presence of bimodal pore distribution in combination with collagen/sHya	1292:1362	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	8	20	from	presence	1292:1299	arg1	combination					1333:1343	combination	1333:1343	combination with collagen/sHya	1333:1362	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	0	21	from	scaffolds	99:107	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells	0:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	7	22	theme	blue	1250:1253	arg1	staining					1255:1262	Sirius red and toluidine blue staining	1225:1262	Sirius red and toluidine blue staining	1225:1262	The scaffolds were successfully coated with a homogenous layer of aECM as shown by Sirius red and toluidine blue staining.					
30678897	9	23	theme	Runt-related	1573:1584	arg1	factor					1600:1605	Runt-related transcription factor 2	1573:1607	Runt-related transcription factor 2	1573:1607	However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II.					
30678897	1	24	theme	main	176:179	arg1	objective					181:189	The main objective	172:189	The main objective of this study	172:203	The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering.					
30678897	5	25	theme	PLGA	984:987	arg1	scaffolds					989:997	the PLGA scaffolds	980:997	the PLGA scaffolds	980:997	The application of two porogens resulted in bimodal pore distribution within the PLGA scaffolds as shown by scanning electron microscopy and microcomputer tomography.					
30678897	4	26	theme	extracellular	805:817	arg1	aECM					829:832	aECM	829:832	aECM	829:832	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	4	26	theme	extracellular	805:817	arg1	matrices					819:826	artificial extracellular matrices	794:826	artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya)	794:900	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	0	27	theme	osteogenic	112:121	arg1	differentiation					123:137	osteogenic differentiation	112:137	osteogenic differentiation of human mesenchymal stem cells	112:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	2	28	theme	stem	487:490	arg1	hMSC					499:502	hMSC	499:502	hMSC	499:502	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	2	28	theme	stem	487:490	arg1	cells					492:496	human mesenchymal stem cells	469:496	human mesenchymal stem cells (hMSC)	469:503	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	4	29	theme	porogen	759:765	arg1	leaching					767:774	porogen leaching	759:774	porogen leaching	759:774	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	7	30	theme	aECM	1208:1211	arg1	layer					1199:1203	a homogenous layer	1186:1203	a homogenous layer of aECM	1186:1211	The scaffolds were successfully coated with a homogenous layer of aECM as shown by Sirius red and toluidine blue staining.					
30678897	1	31	theme	tissue	290:295	arg1	engineering					297:307	bone tissue engineering	285:307	bone tissue engineering	285:307	The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering.					
30678897	4	32	mod	modified	780:787	arg1	scaffolds					642:650	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds	606:650	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution	606:695	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	4	32	mod	modified	780:787	arg3	matrices					819:826	artificial extracellular matrices	794:826	artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya)	794:900	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	4	32	mod	modified	780:787	arg3	aECM					829:832	aECM	829:832	aECM	829:832	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	5	33	theme	pore	955:958	arg1	distribution					960:971	bimodal pore distribution	947:971	bimodal pore distribution within the PLGA scaffolds	947:997	The application of two porogens resulted in bimodal pore distribution within the PLGA scaffolds as shown by scanning electron microscopy and microcomputer tomography.					
30678897	6	34	theme	pores	1083:1087	arg1	types					1074:1078	Two types	1070:1078	Two types of pores with diameters 400-600 μm and 2-20 μm	1070:1125	Two types of pores with diameters 400-600 μm and 2-20 μm were obtained.					
30678897	2	35	theme	human	469:473	arg1	hMSC					499:502	hMSC	499:502	hMSC	499:502	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	2	35	theme	human	469:473	arg1	cells					492:496	human mesenchymal stem cells	469:496	human mesenchymal stem cells (hMSC)	469:503	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	8	36	theme	early	1419:1423	arg1	differentiation					1436:1450	early osteogenic differentiation	1419:1450	early osteogenic differentiation	1419:1450	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	0	37	theme	pore	30:33	arg1	distribution					35:46	bimodal pore distribution	22:46	bimodal pore distribution	22:46	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	0	38	theme	extracellular	63:75	arg1	matrices					77:84	artificial extracellular matrices	52:84	artificial extracellular matrices	52:84	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	2	39	theme	surface	362:368	arg1	modification					370:381	surface modification	362:381	surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC)	362:503	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	9	40	theme	sialoprotein	1631:1642	arg1	expression					1559:1568	the expression	1555:1568	mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II	1529:1645	However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II.					
30678897	9	40	theme	sialoprotein	1631:1642	arg1	mineralization					1529:1542	mineralization	1529:1542	mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II	1529:1645	However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II.					
30678897	4	41	with	scaffolds	642:650	arg1	distribution					684:695	bimodal pore distribution	671:695	bimodal pore distribution	671:695	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	4	41	with	scaffolds	642:650	arg1	monomodal					657:665	monomodal	657:665	monomodal	657:665	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	1	42	theme	biological	224:233	arg1	performance					235:245	the biological performance	220:245	the biological performance of resorbable polymeric scaffolds for bone tissue engineering	220:307	The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering.					
30678897	3	43	theme	90	551:552	arg1	%					553:553	%	553:553	%	553:553	Moreover, a new cell seeding method assuring 90% seeding efficiency on the scaffolds was developed.					
30678897	8	44	theme	pore	1489:1492	arg1	distribution					1494:1505	monomodal pore distribution	1479:1505	monomodal pore distribution	1479:1505	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	3	45	theme	seeding	527:533	arg1	method					535:540	a new cell seeding method	516:540	a new cell seeding method assuring 90% seeding efficiency on the scaffolds	516:589	Moreover, a new cell seeding method assuring 90% seeding efficiency on the scaffolds was developed.					
30678897	5	46	theme	electron	1020:1027	arg1	microscopy					1029:1038	scanning electron microscopy	1011:1038	scanning electron microscopy	1011:1038	The application of two porogens resulted in bimodal pore distribution within the PLGA scaffolds as shown by scanning electron microscopy and microcomputer tomography.					
30678897	7	47	theme	red	1232:1234	arg1	staining					1255:1262	Sirius red and toluidine blue staining	1225:1262	Sirius red and toluidine blue staining	1225:1262	The scaffolds were successfully coated with a homogenous layer of aECM as shown by Sirius red and toluidine blue staining.					
30678897	3	48	theme	new	518:520	arg1	method					535:540	a new cell seeding method	516:540	a new cell seeding method assuring 90% seeding efficiency on the scaffolds	516:589	Moreover, a new cell seeding method assuring 90% seeding efficiency on the scaffolds was developed.					
30678897	1	49	theme	polymeric	261:269	arg1	scaffolds					271:279	resorbable polymeric scaffolds	250:279	resorbable polymeric scaffolds	250:279	The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering.					
30678897	3	50	theme	%	553:553	arg1	efficiency					563:572	90% seeding efficiency	551:572	90% seeding efficiency	551:572	Moreover, a new cell seeding method assuring 90% seeding efficiency on the scaffolds was developed.					
30678897	10	51	theme	bone	1786:1789	arg1	regeneration					1791:1802	bone regeneration	1786:1802	bone regeneration	1786:1802	As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.					
30678897	10	52	theme	PLGA	1660:1663	arg1	result					1653:1658	a result	1651:1658	a result	1651:1658	As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.					
30678897	10	52	theme	PLGA	1660:1663	arg1	material					1767:1774	prospective material	1755:1774	prospective material promoting bone regeneration	1755:1802	As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.					
30678897	10	52	theme	PLGA	1660:1663	arg1	scaffolds					1665:1673	PLGA scaffolds	1660:1673	PLGA scaffolds with bimodal pore distribution modified with collagen/sHya	1660:1732	As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.					
30678897	4	53	theme	high	869:872	arg1	sHya					896:899	sHya	896:899	sHya	896:899	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	4	53	theme	high	869:872	arg1	hyaluronan					884:893	high sulphated hyaluronan	869:893	high sulphated hyaluronan (sHya)	869:900	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	8	54	theme	In	1265:1266	arg1	study					1274:1278	In vitro study	1265:1278	In vitro study	1265:1278	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	0	55	theme	human	142:146	arg1	cells					165:169	human mesenchymal stem cells	142:169	human mesenchymal stem cells	142:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	2	56	theme	scaffolds	390:398	arg1	microstructure					343:356	microstructure	343:356	microstructure	343:356	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	2	56	theme	scaffolds	390:398	arg1	modification					370:381	surface modification	362:381	surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC)	362:503	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	9	57	theme	factor	1600:1605	arg1	expression					1559:1568	the expression	1555:1568	mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II	1529:1645	However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II.					
30678897	9	57	theme	factor	1600:1605	arg1	mineralization					1529:1542	mineralization	1529:1542	mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II	1529:1645	However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II.					
30678897	10	58	theme	pore	1688:1691	arg1	distribution					1693:1704	bimodal pore distribution	1680:1704	bimodal pore distribution	1680:1704	As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.					
30678897	0	59	theme	stem	160:163	arg1	cells					165:169	human mesenchymal stem cells	142:169	human mesenchymal stem cells	142:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	4	60	theme	bimodal	671:677	arg1	distribution					684:695	bimodal pore distribution	671:695	bimodal pore distribution	671:695	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	0	61	from	matrices	77:84	arg1	scaffolds					99:107	polymeric scaffolds	89:107	polymeric scaffolds	89:107	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	4	62	theme	solvent	714:720	arg1	separation					736:745	solvent casting/phase separation	714:745	solvent casting/phase separation followed by porogen leaching	714:774	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	8	63	with	scaffolds	1464:1472	arg1	distribution					1494:1505	monomodal pore distribution	1479:1505	monomodal pore distribution	1479:1505	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	8	64	theme	pore	1312:1315	arg1	distribution					1317:1328	bimodal pore distribution	1304:1328	bimodal pore distribution	1304:1328	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	0	65	theme	matrices	77:84	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells	0:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	0	66	theme	polymeric	89:97	arg1	scaffolds					99:107	polymeric scaffolds	89:107	polymeric scaffolds	89:107	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	9	67	theme	transcription	1586:1598	arg1	factor					1600:1605	Runt-related transcription factor 2	1573:1607	Runt-related transcription factor 2	1573:1607	However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II.					
30678897	0	68	from	effect	12:17	arg1	scaffolds					99:107	polymeric scaffolds	89:107	polymeric scaffolds	89:107	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	0	68	from	effect	12:17	arg1	differentiation					123:137	osteogenic differentiation	112:137	osteogenic differentiation of human mesenchymal stem cells	112:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	9	69	theme	osteopontin	1610:1620	arg1	expression					1559:1568	the expression	1555:1568	mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II	1529:1645	However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II.					
30678897	9	69	theme	osteopontin	1610:1620	arg1	mineralization					1529:1542	mineralization	1529:1542	mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II	1529:1645	However, it enhanced mineralization as well as the expression of Runt-related transcription factor 2, osteopontin and bone sialoprotein II.					
30678897	0	70	from	distribution	35:46	arg1	scaffolds					99:107	polymeric scaffolds	89:107	polymeric scaffolds	89:107	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	4	71	theme	artificial	794:803	arg1	aECM					829:832	aECM	829:832	aECM	829:832	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	4	71	theme	artificial	794:803	arg1	matrices					819:826	artificial extracellular matrices	794:826	artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya)	794:900	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	5	72	theme	bimodal	947:953	arg1	distribution					960:971	bimodal pore distribution	947:971	bimodal pore distribution within the PLGA scaffolds	947:997	The application of two porogens resulted in bimodal pore distribution within the PLGA scaffolds as shown by scanning electron microscopy and microcomputer tomography.					
30678897	2	73	theme	mesenchymal	475:485	arg1	hMSC					499:502	hMSC	499:502	hMSC	499:502	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	2	73	theme	mesenchymal	475:485	arg1	cells					492:496	human mesenchymal stem cells	469:496	human mesenchymal stem cells (hMSC)	469:503	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	8	74	theme	hMSC	1396:1399	arg1	proliferation					1401:1413	hMSC proliferation	1396:1413	hMSC proliferation	1396:1413	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	0	75	theme	bimodal	22:28	arg1	distribution					35:46	bimodal pore distribution	22:46	bimodal pore distribution	22:46	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	6	76	dep	diameters	1094:1102	arg1	diameters					1094:1102	diameters 400-600 μm and 2-20 μm	1094:1125	diameters 400-600 μm and 2-20 μm	1094:1125	Two types of pores with diameters 400-600 μm and 2-20 μm were obtained.					
30678897	6	76	dep	diameters	1094:1102	arg1	2-20 μm					1119:1125	2-20 μm	1119:1125	2-20 μm	1119:1125	Two types of pores with diameters 400-600 μm and 2-20 μm were obtained.					
30678897	6	76	dep	diameters	1094:1102	arg1	400-600 μm					1104:1113	400-600 μm	1104:1113	400-600 μm	1104:1113	Two types of pores with diameters 400-600 μm and 2-20 μm were obtained.					
30678897	2	77	theme	osteogenic	439:448	arg1	differentiation					450:464	osteogenic differentiation	439:464	osteogenic differentiation	439:464	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	0	78	theme	distribution	35:46	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells	0:169	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	6	79	with	pores	1083:1087	arg1	diameters					1094:1102	diameters 400-600 μm and 2-20 μm	1094:1125	diameters 400-600 μm and 2-20 μm	1094:1125	Two types of pores with diameters 400-600 μm and 2-20 μm were obtained.					
30678897	6	79	with	pores	1083:1087	arg1	2-20 μm					1119:1125	2-20 μm	1119:1125	2-20 μm	1119:1125	Two types of pores with diameters 400-600 μm and 2-20 μm were obtained.					
30678897	6	79	with	pores	1083:1087	arg1	400-600 μm					1104:1113	400-600 μm	1104:1113	400-600 μm	1104:1113	Two types of pores with diameters 400-600 μm and 2-20 μm were obtained.					
30678897	8	80	theme	osteogenic	1425:1434	arg1	differentiation					1436:1450	early osteogenic differentiation	1419:1450	early osteogenic differentiation	1419:1450	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	0	81	theme	artificial	52:61	arg1	matrices					77:84	artificial extracellular matrices	52:84	artificial extracellular matrices	52:84	Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.					
30678897	5	82	theme	microcomputer	1044:1056	arg1	tomography					1058:1067	microcomputer tomography	1044:1067	microcomputer tomography	1044:1067	The application of two porogens resulted in bimodal pore distribution within the PLGA scaffolds as shown by scanning electron microscopy and microcomputer tomography.					
30678897	2	83	theme	cells	492:496	arg1	adhesion					411:418	adhesion	411:418	adhesion	411:418	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	2	83	theme	cells	492:496	arg1	proliferation					421:433	proliferation	421:433	proliferation	421:433	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	2	83	theme	cells	492:496	arg1	differentiation					450:464	osteogenic differentiation	439:464	osteogenic differentiation	439:464	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	4	84	theme	collagen	849:856	arg1	type					858:861	collagen type I	849:863	collagen type I	849:863	Poly(l‑lactide‑co‑glycolide) (PLGA) scaffolds with monomodal and bimodal pore distribution were produced by solvent casting/phase separation followed by porogen leaching and modified with artificial extracellular matrices (aECM) consisting of collagen type I and high sulphated hyaluronan (sHya).					
30678897	8	85	with	combination	1333:1343	arg1	collagen/sHya					1350:1362	collagen/sHya	1350:1362	collagen/sHya	1350:1362	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	10	86	theme	prospective	1755:1765	arg1	material					1767:1774	prospective material	1755:1774	prospective material promoting bone regeneration	1755:1802	As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.					
30678897	10	86	theme	prospective	1755:1765	arg1	scaffolds					1665:1673	PLGA scaffolds	1660:1673	PLGA scaffolds with bimodal pore distribution modified with collagen/sHya	1660:1732	As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.					
30678897	5	87	theme	scanning	1011:1018	arg1	microscopy					1029:1038	scanning electron microscopy	1011:1038	scanning electron microscopy	1011:1038	The application of two porogens resulted in bimodal pore distribution within the PLGA scaffolds as shown by scanning electron microscopy and microcomputer tomography.					
30678897	10	88	with	scaffolds	1665:1673	arg1	distribution					1693:1704	bimodal pore distribution	1680:1704	bimodal pore distribution	1680:1704	As a result PLGA scaffolds with bimodal pore distribution modified with collagen/sHya can be considered as prospective material promoting bone regeneration.					
30678897	8	89	theme	monomodal	1479:1487	arg1	distribution					1494:1505	monomodal pore distribution	1479:1505	monomodal pore distribution	1479:1505	In vitro study showed that presence of bimodal pore distribution in combination with collagen/sHya did not significantly influence hMSC proliferation and early osteogenic differentiation compared to scaffolds with monomodal pore distribution.					
30678897	2	90	mod	modification	370:381	arg3	surface					362:368	surface modification	362:381	surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC)	362:503	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	2	90	mod	modification	370:381	arg1	scaffolds					390:398	the scaffolds	386:398	the scaffolds	386:398	Specifically, we focused on both microstructure and surface modification of the scaffolds to augment adhesion, proliferation and osteogenic differentiation of human mesenchymal stem cells (hMSC).					
30678897	1	91	theme	resorbable	250:259	arg1	scaffolds					271:279	resorbable polymeric scaffolds	250:279	resorbable polymeric scaffolds	250:279	The main objective of this study was to enhance the biological performance of resorbable polymeric scaffolds for bone tissue engineering.					
31761152	3	0	theme	%	676:676	arg1	concentrations					635:648	CHAp concentrations	630:648	CHAp concentrations of 0, 5, 10, 15, and 20 wt%	630:676	CHAp nanoparticles were synthesized using a co-precipitation method, and nanofibrous PVA/CS/CHAp scaffolds were fabricated by electrospinning using CHAp concentrations of 0, 5, 10, 15, and 20 wt%.					
31761152	15	1	contain	has	2050:2052	arg2	potential					2054:2062	potential	2054:2062	potential	2054:2062	PVA/CS/CHAp has potential to serve as an alternative scaffold material for bone tissue engineering.					
31761152	15	1	contain	has	2050:2052	arg1	PVA/CS/CHAp					2038:2048	PVA/CS/CHAp	2038:2048	PVA/CS/CHAp	2038:2048	PVA/CS/CHAp has potential to serve as an alternative scaffold material for bone tissue engineering.					
31761152	6	2	theme	great	1100:1104	arg1	agglomerations					1106:1119	great agglomerations	1100:1119	great agglomerations	1100:1119	The addition of CHAp to the composite decreased the fiber diameter from ∼160 nm at 0 wt% to ∼139 nm at 15 wt% and great agglomerations were evident at 20 wt%.					
31761152	13	3	theme	morphology	1839:1848	arg1	results					1850:1856	Cell viability and cell morphology results	1815:1856	Cell viability and cell morphology results	1815:1856	Cell viability and cell morphology results reveal that PVA/CS/CHAp able to facilitate osteoblast cells to attach and proliferate.					
31761152	11	4	theme	Protein	1560:1566	arg1	adsorption					1568:1577	Protein adsorption	1560:1577	Protein adsorption onto the scaffold	1560:1595	Protein adsorption onto the scaffold increased 2.3fold at 20 wt% when compared to 0 wt%.					
31761152	1	5	from	challenge	306:314	arg1	today					343:347	bone tissue engineering today	319:347	bone tissue engineering today	319:347	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	3	6	theme	PVA/CS/CHAp	567:577	arg1	scaffolds					579:587	nanofibrous PVA/CS/CHAp scaffolds	555:587	nanofibrous PVA/CS/CHAp scaffolds	555:587	CHAp nanoparticles were synthesized using a co-precipitation method, and nanofibrous PVA/CS/CHAp scaffolds were fabricated by electrospinning using CHAp concentrations of 0, 5, 10, 15, and 20 wt%.					
31761152	1	7	theme	bone	185:188	arg1	structure					172:180	the physicochemical structure	152:180	the physicochemical structure of bone at the nanoscale level	152:211	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	4	8	theme	tensile	819:825	arg1	tests					836:840	tensile strength tests	819:840	tensile strength tests	819:840	The physicochemical properties of the scaffolds were evaluated by SEM, XRD, FTIR, and EDS, and the mechanical properties were determined by tensile strength tests.					
31761152	5	9	theme	Swelling	843:850	arg1	behavior					852:859	Swelling behavior	843:859	Swelling behavior	843:859	Swelling behavior, protein adsorption onto the scaffold surfaces, surface biomineralization, and cells viability were also evaluated in vitro.					
31761152	12	10	theme	fluid	1788:1792	arg1	solution					1794:1801	a simulated body fluid solution	1771:1801	a simulated body fluid solution for 7 days	1771:1812	The PVA/CS/CHAp 15 wt% showed a better bioactivity when compared to PVA/CS/CHAp 0 wt% after immersion of the scaffolds in a simulated body fluid solution for 7 days.					
31761152	8	11	theme	average	1342:1348	arg1	MPa					1357:1359	an average 103.86 MPa	1339:1359	an average 103.86 MPa	1339:1359	This CHAp incorporation significantly increased the modulus of the scaffold at PVA/CS/CHAp 15 wt%, with an average 103.86 MPa, but tensile strength was not significantly altered.					
31761152	6	12	theme	wt	1140:1141	arg1	%					1142:1142	20 wt%	1137:1142	20 wt%	1137:1142	The addition of CHAp to the composite decreased the fiber diameter from ∼160 nm at 0 wt% to ∼139 nm at 15 wt% and great agglomerations were evident at 20 wt%.					
31761152	10	13	theme	Swelling	1498:1505	arg1	capacity					1507:1514	Swelling capacity	1498:1514	Swelling capacity of scaffold	1498:1526	Swelling capacity of scaffold increases due to CHAp addition.					
31761152	4	14	theme	scaffolds	717:725	arg1	properties					699:708	The physicochemical properties	679:708	The physicochemical properties of the scaffolds	679:725	The physicochemical properties of the scaffolds were evaluated by SEM, XRD, FTIR, and EDS, and the mechanical properties were determined by tensile strength tests.					
31761152	6	15	from	%	1142:1142	arg1	evident					1126:1132	evident	1126:1132	evident	1126:1132	The addition of CHAp to the composite decreased the fiber diameter from ∼160 nm at 0 wt% to ∼139 nm at 15 wt% and great agglomerations were evident at 20 wt%.					
31761152	0	16	theme	carbonated	51:60	arg1	scaffold					91:98	carbonated hydroxyapatite nanoparticles scaffold	51:98	carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering	51:126	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	1	17	theme	tissue	324:329	arg1	engineering					331:341	bone tissue engineering	319:341	bone tissue engineering today	319:347	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	4	18	theme	physicochemical	683:697	arg1	properties					699:708	The physicochemical properties	679:708	The physicochemical properties of the scaffolds	679:725	The physicochemical properties of the scaffolds were evaluated by SEM, XRD, FTIR, and EDS, and the mechanical properties were determined by tensile strength tests.					
31761152	0	19	theme	nanoparticles	77:89	arg1	scaffold					91:98	carbonated hydroxyapatite nanoparticles scaffold	51:98	carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering	51:126	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	12	20	theme	scaffolds	1758:1766	arg1	immersion					1741:1749	immersion	1741:1749	immersion of the scaffolds	1741:1766	The PVA/CS/CHAp 15 wt% showed a better bioactivity when compared to PVA/CS/CHAp 0 wt% after immersion of the scaffolds in a simulated body fluid solution for 7 days.					
31761152	8	21	theme	CHAp	1240:1243	arg1	incorporation					1245:1257	This CHAp incorporation	1235:1257	This CHAp incorporation	1235:1257	This CHAp incorporation significantly increased the modulus of the scaffold at PVA/CS/CHAp 15 wt%, with an average 103.86 MPa, but tensile strength was not significantly altered.					
31761152	5	22	theme	cells	940:944	arg1	viability					946:954	cells viability	940:954	cells viability	940:954	Swelling behavior, protein adsorption onto the scaffold surfaces, surface biomineralization, and cells viability were also evaluated in vitro.					
31761152	14	23	theme	higher	1957:1962	arg1	CHAp					1964:1967	higher CHAp	1957:1967	higher CHAp	1957:1967	Introducing higher CHAp into the scaffold could increase the cell viability of the scaffold.					
31761152	2	24	theme	nanofibrous	391:401	arg1	scaffold					472:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	This work describes the fabrication of a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold.					
31761152	0	25	theme	tissue	109:114	arg1	engineering					116:126	bone tissue engineering	104:126	bone tissue engineering	104:126	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	6	26	theme	fiber	1038:1042	arg1	diameter					1044:1051	the fiber diameter	1034:1051	the fiber diameter from ∼160 nm	1034:1064	The addition of CHAp to the composite decreased the fiber diameter from ∼160 nm at 0 wt% to ∼139 nm at 15 wt% and great agglomerations were evident at 20 wt%.					
31761152	0	27	theme	Nanofibrous	0:10	arg1	poly					12:15	Nanofibrous poly	0:15	Nanofibrous poly(vinyl alcohol)/chitosan	0:39	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	0	27	theme	Nanofibrous	0:10	arg1	alcohol					23:29	vinyl alcohol	17:29	vinyl alcohol	17:29	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	5	28	theme	surface	909:915	arg1	biomineralization					917:933	surface biomineralization	909:933	surface biomineralization	909:933	Swelling behavior, protein adsorption onto the scaffold surfaces, surface biomineralization, and cells viability were also evaluated in vitro.					
31761152	7	29	theme	nanofibrous	1212:1222	arg1	structure					1224:1232	the nanofibrous structure	1208:1232	the nanofibrous structure	1208:1232	XRD, FTIR, and EDS showed effective incorporation of CHAp into the nanofibrous structure.					
31761152	2	30	theme	/chitosan/carbonated	422:441	arg1	scaffold					472:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	This work describes the fabrication of a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold.					
31761152	2	31	theme	alcohol	414:420	arg1	scaffold					472:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	This work describes the fabrication of a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold.					
31761152	15	32	theme	bone	2113:2116	arg1	engineering					2125:2135	bone tissue engineering	2113:2135	bone tissue engineering	2113:2135	PVA/CS/CHAp has potential to serve as an alternative scaffold material for bone tissue engineering.					
31761152	12	33	theme	PVA/CS/CHAp	1717:1727	arg1	%					1733:1733	PVA/CS/CHAp 0 wt%	1717:1733	PVA/CS/CHAp 0 wt%	1717:1733	The PVA/CS/CHAp 15 wt% showed a better bioactivity when compared to PVA/CS/CHAp 0 wt% after immersion of the scaffolds in a simulated body fluid solution for 7 days.					
31761152	8	34	theme	scaffold	1302:1309	arg1	modulus					1287:1293	the modulus	1283:1293	the modulus of the scaffold	1283:1309	This CHAp incorporation significantly increased the modulus of the scaffold at PVA/CS/CHAp 15 wt%, with an average 103.86 MPa, but tensile strength was not significantly altered.					
31761152	1	35	theme	main	301:304	arg1	challenge					306:314	a main challenge	299:314	a main challenge in bone tissue engineering today	299:347	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	12	36	theme	wt	1731:1732	arg1	%					1733:1733	PVA/CS/CHAp 0 wt%	1717:1733	PVA/CS/CHAp 0 wt%	1717:1733	The PVA/CS/CHAp 15 wt% showed a better bioactivity when compared to PVA/CS/CHAp 0 wt% after immersion of the scaffolds in a simulated body fluid solution for 7 days.					
31761152	6	37	theme	CHAp	1002:1005	arg1	addition					990:997	The addition	986:997	The addition of CHAp to the composite	986:1022	The addition of CHAp to the composite decreased the fiber diameter from ∼160 nm at 0 wt% to ∼139 nm at 15 wt% and great agglomerations were evident at 20 wt%.					
31761152	8	38	theme	PVA/CS/CHAp	1314:1324	arg1	%					1331:1331	PVA/CS/CHAp 15 wt%	1314:1331	PVA/CS/CHAp 15 wt%	1314:1331	This CHAp incorporation significantly increased the modulus of the scaffold at PVA/CS/CHAp 15 wt%, with an average 103.86 MPa, but tensile strength was not significantly altered.					
31761152	15	39	theme	alternative	2079:2089	arg1	material					2100:2107	an alternative scaffold material	2076:2107	an alternative scaffold material for bone tissue engineering	2076:2135	PVA/CS/CHAp has potential to serve as an alternative scaffold material for bone tissue engineering.					
31761152	12	40	theme	better	1681:1686	arg1	bioactivity					1688:1698	a better bioactivity	1679:1698	a better bioactivity when compared to PVA/CS/CHAp 0 wt%	1679:1733	The PVA/CS/CHAp 15 wt% showed a better bioactivity when compared to PVA/CS/CHAp 0 wt% after immersion of the scaffolds in a simulated body fluid solution for 7 days.					
31761152	7	41	theme	effective	1171:1179	arg1	incorporation					1181:1193	effective incorporation	1171:1193	effective incorporation of CHAp into the nanofibrous structure	1171:1232	XRD, FTIR, and EDS showed effective incorporation of CHAp into the nanofibrous structure.					
31761152	11	42	theme	wt	1621:1622	arg1	%					1623:1623	20 wt%	1618:1623	20 wt%	1618:1623	Protein adsorption onto the scaffold increased 2.3fold at 20 wt% when compared to 0 wt%.					
31761152	8	43	theme	wt	1329:1330	arg1	%					1331:1331	PVA/CS/CHAp 15 wt%	1314:1331	PVA/CS/CHAp 15 wt%	1314:1331	This CHAp incorporation significantly increased the modulus of the scaffold at PVA/CS/CHAp 15 wt%, with an average 103.86 MPa, but tensile strength was not significantly altered.					
31761152	3	44	theme	CHAp	482:485	arg1	nanoparticles					487:499	CHAp nanoparticles	482:499	CHAp nanoparticles	482:499	CHAp nanoparticles were synthesized using a co-precipitation method, and nanofibrous PVA/CS/CHAp scaffolds were fabricated by electrospinning using CHAp concentrations of 0, 5, 10, 15, and 20 wt%.					
31761152	1	45	theme	attractive	219:228	arg1	scaffold					131:138	A scaffold	129:138	A scaffold that mimics the physicochemical structure of bone at the nanoscale level	129:211	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	1	45	theme	attractive	219:228	arg1	alternative					230:240	an attractive alternative	216:240	an attractive alternative to conventional bone grafts	216:268	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	12	46	theme	PVA/CS/CHAp	1653:1663	arg1	%					1670:1670	The PVA/CS/CHAp 15 wt%	1649:1670	The PVA/CS/CHAp 15 wt%	1649:1670	The PVA/CS/CHAp 15 wt% showed a better bioactivity when compared to PVA/CS/CHAp 0 wt% after immersion of the scaffolds in a simulated body fluid solution for 7 days.					
31761152	3	47	theme	co-precipitation	526:541	arg1	method					543:548	a co-precipitation method	524:548	a co-precipitation method	524:548	CHAp nanoparticles were synthesized using a co-precipitation method, and nanofibrous PVA/CS/CHAp scaffolds were fabricated by electrospinning using CHAp concentrations of 0, 5, 10, 15, and 20 wt%.					
31761152	12	48	theme	wt	1668:1669	arg1	%					1670:1670	The PVA/CS/CHAp 15 wt%	1649:1670	The PVA/CS/CHAp 15 wt%	1649:1670	The PVA/CS/CHAp 15 wt% showed a better bioactivity when compared to PVA/CS/CHAp 0 wt% after immersion of the scaffolds in a simulated body fluid solution for 7 days.					
31761152	6	49	from	nm	1063:1064	arg1	diameter					1044:1051	the fiber diameter	1034:1051	the fiber diameter from ∼160 nm	1034:1064	The addition of CHAp to the composite decreased the fiber diameter from ∼160 nm at 0 wt% to ∼139 nm at 15 wt% and great agglomerations were evident at 20 wt%.					
31761152	1	50	theme	bone	258:261	arg1	grafts					263:268	conventional bone grafts	245:268	conventional bone grafts	245:268	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	4	51	theme	strength	827:834	arg1	tests					836:840	tensile strength tests	819:840	tensile strength tests	819:840	The physicochemical properties of the scaffolds were evaluated by SEM, XRD, FTIR, and EDS, and the mechanical properties were determined by tensile strength tests.					
31761152	0	52	contain	contained	41:49	arg2	scaffold					91:98	carbonated hydroxyapatite nanoparticles scaffold	51:98	carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering	51:126	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	0	52	contain	contained	41:49	arg1	/chitosan					31:39	Nanofibrous poly(vinyl alcohol)/chitosan	0:39	Nanofibrous poly(vinyl alcohol)/chitosan	0:39	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	3	53	theme	nanofibrous	555:565	arg1	scaffolds					579:587	nanofibrous PVA/CS/CHAp scaffolds	555:587	nanofibrous PVA/CS/CHAp scaffolds	555:587	CHAp nanoparticles were synthesized using a co-precipitation method, and nanofibrous PVA/CS/CHAp scaffolds were fabricated by electrospinning using CHAp concentrations of 0, 5, 10, 15, and 20 wt%.					
31761152	5	54	theme	protein	862:868	arg1	adsorption					870:879	protein adsorption	862:879	protein adsorption onto the scaffold surfaces	862:906	Swelling behavior, protein adsorption onto the scaffold surfaces, surface biomineralization, and cells viability were also evaluated in vitro.					
31761152	13	55	theme	Cell	1815:1818	arg1	viability					1820:1828	Cell viability	1815:1828	Cell viability	1815:1828	Cell viability and cell morphology results reveal that PVA/CS/CHAp able to facilitate osteoblast cells to attach and proliferate.					
31761152	6	56	theme	wt	1092:1093	arg1	%					1094:1094	15 wt%	1089:1094	15 wt%	1089:1094	The addition of CHAp to the composite decreased the fiber diameter from ∼160 nm at 0 wt% to ∼139 nm at 15 wt% and great agglomerations were evident at 20 wt%.					
31761152	14	57	theme	scaffold	2028:2035	arg1	viability					2011:2019	the cell viability	2002:2019	the cell viability of the scaffold	2002:2035	Introducing higher CHAp into the scaffold could increase the cell viability of the scaffold.					
31761152	1	58	theme	nanoscale	197:205	arg1	level					207:211	the nanoscale level	193:211	the nanoscale level	193:211	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	10	59	theme	scaffold	1519:1526	arg1	capacity					1507:1514	Swelling capacity	1498:1514	Swelling capacity of scaffold	1498:1526	Swelling capacity of scaffold increases due to CHAp addition.					
31761152	3	60	theme	CHAp	630:633	arg1	concentrations					635:648	CHAp concentrations	630:648	CHAp concentrations of 0, 5, 10, 15, and 20 wt%	630:676	CHAp nanoparticles were synthesized using a co-precipitation method, and nanofibrous PVA/CS/CHAp scaffolds were fabricated by electrospinning using CHAp concentrations of 0, 5, 10, 15, and 20 wt%.					
31761152	12	61	theme	body	1783:1786	arg1	solution					1794:1801	a simulated body fluid solution	1771:1801	a simulated body fluid solution for 7 days	1771:1812	The PVA/CS/CHAp 15 wt% showed a better bioactivity when compared to PVA/CS/CHAp 0 wt% after immersion of the scaffolds in a simulated body fluid solution for 7 days.					
31761152	8	62	theme	103.86	1350:1355	arg1	MPa					1357:1359	an average 103.86 MPa	1339:1359	an average 103.86 MPa	1339:1359	This CHAp incorporation significantly increased the modulus of the scaffold at PVA/CS/CHAp 15 wt%, with an average 103.86 MPa, but tensile strength was not significantly altered.					
31761152	15	63	theme	scaffold	2091:2098	arg1	material					2100:2107	an alternative scaffold material	2076:2107	an alternative scaffold material for bone tissue engineering	2076:2135	PVA/CS/CHAp has potential to serve as an alternative scaffold material for bone tissue engineering.					
31761152	3	64	theme	wt	674:675	arg1	%					676:676	0, 5, 10, 15, and 20 wt%	653:676	0, 5, 10, 15, and 20 wt%	653:676	CHAp nanoparticles were synthesized using a co-precipitation method, and nanofibrous PVA/CS/CHAp scaffolds were fabricated by electrospinning using CHAp concentrations of 0, 5, 10, 15, and 20 wt%.					
31761152	1	65	from	level	207:211	arg1	structure					172:180	the physicochemical structure	152:180	the physicochemical structure of bone at the nanoscale level	152:211	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	5	66	theme	scaffold	890:897	arg1	surfaces					899:906	the scaffold surfaces	886:906	the scaffold surfaces	886:906	Swelling behavior, protein adsorption onto the scaffold surfaces, surface biomineralization, and cells viability were also evaluated in vitro.					
31761152	8	67	theme	tensile	1366:1372	arg1	strength					1374:1381	tensile strength	1366:1381	tensile strength	1366:1381	This CHAp incorporation significantly increased the modulus of the scaffold at PVA/CS/CHAp 15 wt%, with an average 103.86 MPa, but tensile strength was not significantly altered.					
31761152	0	68	theme	hydroxyapatite	62:75	arg1	scaffold					91:98	carbonated hydroxyapatite nanoparticles scaffold	51:98	carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering	51:126	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	1	69	theme	engineering	331:341	arg1	today					343:347	bone tissue engineering today	319:347	bone tissue engineering today	319:347	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	1	70	theme	physicochemical	156:170	arg1	structure					172:180	the physicochemical structure	152:180	the physicochemical structure of bone at the nanoscale level	152:211	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	9	71	from	break	1441:1445	arg1	elongation					1427:1436	the elongation	1423:1436	the elongation at break	1423:1445	However, the elongation at break was decreased as the CHAp concentration increased.					
31761152	10	72	theme	CHAp	1545:1548	arg1	addition					1550:1557	CHAp addition	1545:1557	CHAp addition	1545:1557	Swelling capacity of scaffold increases due to CHAp addition.					
31761152	0	73	theme	bone	104:107	arg1	engineering					116:126	bone tissue engineering	104:126	bone tissue engineering	104:126	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	12	74	theme	simulated	1773:1781	arg1	solution					1794:1801	a simulated body fluid solution	1771:1801	a simulated body fluid solution for 7 days	1771:1812	The PVA/CS/CHAp 15 wt% showed a better bioactivity when compared to PVA/CS/CHAp 0 wt% after immersion of the scaffolds in a simulated body fluid solution for 7 days.					
31761152	11	75	theme	wt	1644:1645	arg1	%					1646:1646	0 wt%	1642:1646	0 wt%	1642:1646	Protein adsorption onto the scaffold increased 2.3fold at 20 wt% when compared to 0 wt%.					
31761152	14	76	theme	cell	2006:2009	arg1	viability					2011:2019	the cell viability	2002:2019	the cell viability of the scaffold	2002:2035	Introducing higher CHAp into the scaffold could increase the cell viability of the scaffold.					
31761152	0	77	theme	poly	12:15	arg1	/chitosan					31:39	Nanofibrous poly(vinyl alcohol)/chitosan	0:39	Nanofibrous poly(vinyl alcohol)/chitosan	0:39	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	13	78	theme	osteoblast	1901:1910	arg1	cells					1912:1916	osteoblast cells	1901:1916	osteoblast cells	1901:1916	Cell viability and cell morphology results reveal that PVA/CS/CHAp able to facilitate osteoblast cells to attach and proliferate.					
31761152	2	79	theme	hydroxyapatite	443:456	arg1	scaffold					472:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	This work describes the fabrication of a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold.					
31761152	0	80	theme	vinyl	17:21	arg1	poly					12:15	Nanofibrous poly	0:15	Nanofibrous poly(vinyl alcohol)/chitosan	0:39	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	0	80	theme	vinyl	17:21	arg1	alcohol					23:29	vinyl alcohol	17:29	vinyl alcohol	17:29	Nanofibrous poly(vinyl alcohol)/chitosan contained carbonated hydroxyapatite nanoparticles scaffold for bone tissue engineering.					
31761152	7	81	theme	CHAp	1198:1201	arg1	incorporation					1181:1193	effective incorporation	1171:1193	effective incorporation of CHAp into the nanofibrous structure	1171:1232	XRD, FTIR, and EDS showed effective incorporation of CHAp into the nanofibrous structure.					
31761152	2	82	theme	vinyl	408:412	arg1	scaffold					472:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	This work describes the fabrication of a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold.					
31761152	15	83	theme	tissue	2118:2123	arg1	engineering					2125:2135	bone tissue engineering	2113:2135	bone tissue engineering	2113:2135	PVA/CS/CHAp has potential to serve as an alternative scaffold material for bone tissue engineering.					
31761152	9	84	theme	CHAp	1468:1471	arg1	concentration					1473:1485	the CHAp concentration	1464:1485	the CHAp concentration	1464:1485	However, the elongation at break was decreased as the CHAp concentration increased.					
31761152	2	85	theme	scaffold	472:479	arg1	fabrication					374:384	the fabrication	370:384	the fabrication of a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	370:479	This work describes the fabrication of a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold.					
31761152	2	86	theme	poly	403:406	arg1	scaffold					472:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	This work describes the fabrication of a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold.					
31761152	2	87	theme	PVA/CS/CHAp	459:469	arg1	scaffold					472:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold	389:479	This work describes the fabrication of a nanofibrous poly(vinyl alcohol)/chitosan/carbonated hydroxyapatite (PVA/CS/CHAp) scaffold.					
31761152	4	88	theme	mechanical	778:787	arg1	properties					789:798	the mechanical properties	774:798	the mechanical properties	774:798	The physicochemical properties of the scaffolds were evaluated by SEM, XRD, FTIR, and EDS, and the mechanical properties were determined by tensile strength tests.					
31761152	1	89	theme	bone	319:322	arg1	engineering					331:341	bone tissue engineering	319:341	bone tissue engineering today	319:347	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
31761152	6	90	theme	wt	1071:1072	arg1	%					1073:1073	0 wt%	1069:1073	0 wt%	1069:1073	The addition of CHAp to the composite decreased the fiber diameter from ∼160 nm at 0 wt% to ∼139 nm at 15 wt% and great agglomerations were evident at 20 wt%.					
31761152	13	91	theme	viability	1820:1828	arg1	results					1850:1856	Cell viability and cell morphology results	1815:1856	Cell viability and cell morphology results	1815:1856	Cell viability and cell morphology results reveal that PVA/CS/CHAp able to facilitate osteoblast cells to attach and proliferate.					
31761152	13	92	theme	cell	1834:1837	arg1	morphology					1839:1848	cell morphology	1834:1848	cell morphology	1834:1848	Cell viability and cell morphology results reveal that PVA/CS/CHAp able to facilitate osteoblast cells to attach and proliferate.					
31761152	1	93	theme	conventional	245:256	arg1	grafts					263:268	conventional bone grafts	245:268	conventional bone grafts	245:268	A scaffold that mimics the physicochemical structure of bone at the nanoscale level is an attractive alternative to conventional bone grafts, but its development remains a main challenge in bone tissue engineering today.					
29901267	0	0	theme	Circulating	91:101	arg1	Levels					111:116	Circulating Insulin Levels	91:116	Circulating Insulin Levels	91:116	Liquid Sucrose Consumption Promotes Obesity and Impairs Glucose Tolerance Without Altering Circulating Insulin Levels.					
29901267	10	1	theme	lean	1153:1156	arg1	mass					1163:1166	lean body mass	1153:1166	lean body mass	1153:1166	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	10	2	theme	significant	1250:1260	arg1	correlation					1262:1272	the most significant correlation	1241:1272	the most significant correlation	1241:1272	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	10	2	theme	significant	1250:1260	arg1	VO2					1234:1236	VO2	1234:1236	VO2	1234:1236	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	10	3	theme	fat-free	1173:1180	arg1	mass					1182:1185	fat-free mass	1173:1185	fat-free mass	1173:1185	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	11	4	from	increase	1433:1440	arg1	insulin					1457:1463	circulating insulin	1445:1463	circulating insulin	1445:1463	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	7	5	theme	circulating	854:864	arg1	levels					874:879	circulating insulin levels	854:879	circulating insulin levels	854:879	These metabolic changes occurred without alterations in circulating insulin levels and despite increases in whole body EE.					
29901267	11	6	from	intake	1310:1315	arg1	mice					1338:1341	mice	1338:1341	mice	1338:1341	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	3	7	theme	sucrose	378:384	arg1	intake					386:391	liquid sucrose intake	371:391	liquid sucrose intake	371:391	This study tested the hypothesis that liquid sucrose intake promotes obesity by increasing serum insulin levels and tissue lipid accumulation.					
29901267	6	8	theme	control	784:790	arg1	mice					792:795	control mice	784:795	control mice	784:795	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	11	9	theme	circulating	1445:1455	arg1	insulin					1457:1463	circulating insulin	1445:1463	circulating insulin	1445:1463	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	7	10	theme	insulin	866:872	arg1	levels					874:879	circulating insulin levels	854:879	circulating insulin levels	854:879	These metabolic changes occurred without alterations in circulating insulin levels and despite increases in whole body EE.					
29901267	0	11	theme	Insulin	103:109	arg1	Levels					111:116	Circulating Insulin Levels	91:116	Circulating Insulin Levels	91:116	Liquid Sucrose Consumption Promotes Obesity and Impairs Glucose Tolerance Without Altering Circulating Insulin Levels.					
29901267	4	12	theme	30	509:510	arg1	%					511:511	%	511:511	%	511:511	METHODS C57BL/6J mice were given 30% sucrose in liquid form.					
29901267	9	13	theme	fat-free	1039:1046	arg1	mass					1048:1051	fat-free mass	1039:1051	fat-free mass	1039:1051	Oxygen consumption (VO2 ) correlated with fat-free mass and moderately with TBM, but not with fat mass.					
29901267	11	14	theme	sucrose	1327:1333	arg1	intake					1310:1315	intake	1310:1315	intake of liquid sucrose in mice	1310:1341	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	5	15	theme	lipid	615:619	arg1	content					621:627	tissue lipid content	608:627	tissue lipid content	608:627	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	5	16	theme	tissue	608:613	arg1	content					621:627	tissue lipid content	608:627	tissue lipid content	608:627	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	2	17	theme	sugar-sweetened	287:301	arg1	beverages					303:311	sugar-sweetened beverages	287:311	sugar-sweetened beverages	287:311	Caloric intake via sugar-sweetened beverages may be influential.					
29901267	5	18	from	Changes	537:543	arg1	EE					599:600	EE	599:600	EE	599:600	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	5	18	from	Changes	537:543	arg1	gain					555:558	weight gain	548:558	weight gain	548:558	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	5	18	from	Changes	537:543	arg1	composition					566:576	body composition	561:576	body composition	561:576	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	5	18	from	Changes	537:543	arg1	content					621:627	tissue lipid content	608:627	tissue lipid content	608:627	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	5	18	from	Changes	537:543	arg1	expenditure					586:596	energy expenditure	579:596	energy expenditure (EE)	579:601	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	1	19	theme	obesity	180:186	arg1	rates					171:175	the rising rates	160:175	the rising rates of obesity	160:186	OBJECTIVE Multiple factors contribute to the rising rates of obesity and to difficulties in weight reduction that exist in the worldwide population.					
29901267	3	20	theme	liquid	371:376	arg1	intake					386:391	liquid sucrose intake	371:391	liquid sucrose intake	371:391	This study tested the hypothesis that liquid sucrose intake promotes obesity by increasing serum insulin levels and tissue lipid accumulation.					
29901267	5	21	theme	energy	579:584	arg1	EE					599:600	EE	599:600	EE	599:600	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	5	21	theme	energy	579:584	arg1	expenditure					586:596	energy expenditure	579:596	energy expenditure (EE)	579:601	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	0	22	theme	Sucrose	7:13	arg1	Consumption					15:25	Liquid Sucrose Consumption	0:25	Liquid Sucrose Consumption	0:25	Liquid Sucrose Consumption Promotes Obesity and Impairs Glucose Tolerance Without Altering Circulating Insulin Levels.					
29901267	4	23	theme	%	511:511	arg1	sucrose					513:519	30% sucrose	509:519	30% sucrose	509:519	METHODS C57BL/6J mice were given 30% sucrose in liquid form.					
29901267	8	24	theme	mice	991:994	arg1	liver					938:942	liver	938:942	liver	938:942	Lipid accrued in liver, but not skeletal muscle, of sucrose-consuming mice.					
29901267	6	25	theme	glucose	754:760	arg1	tolerance					762:770	impaired glucose tolerance	745:770	impaired glucose tolerance relative to control mice	745:795	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	6	26	theme	body	692:695	arg1	mass					697:700	total body mass	686:700	more total body mass (TBM)	681:706	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	6	26	theme	body	692:695	arg1	TBM					703:705	TBM	703:705	TBM	703:705	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	3	27	theme	tissue	449:454	arg1	accumulation					462:473	tissue lipid accumulation	449:473	tissue lipid accumulation	449:473	This study tested the hypothesis that liquid sucrose intake promotes obesity by increasing serum insulin levels and tissue lipid accumulation.					
29901267	0	28	theme	Liquid	0:5	arg1	Consumption					15:25	Liquid Sucrose Consumption	0:25	Liquid Sucrose Consumption	0:25	Liquid Sucrose Consumption Promotes Obesity and Impairs Glucose Tolerance Without Altering Circulating Insulin Levels.					
29901267	6	29	theme	impaired	745:752	arg1	tolerance					762:770	impaired glucose tolerance	745:770	impaired glucose tolerance relative to control mice	745:795	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	7	30	from	increases	893:901	arg1	EE					917:918	whole body EE	906:918	whole body EE	906:918	These metabolic changes occurred without alterations in circulating insulin levels and despite increases in whole body EE.					
29901267	6	31	theme	total	686:690	arg1	mass					697:700	total body mass	686:700	more total body mass (TBM)	681:706	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	6	31	theme	total	686:690	arg1	TBM					703:705	TBM	703:705	TBM	703:705	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	3	32	theme	lipid	456:460	arg1	accumulation					462:473	tissue lipid accumulation	449:473	tissue lipid accumulation	449:473	This study tested the hypothesis that liquid sucrose intake promotes obesity by increasing serum insulin levels and tissue lipid accumulation.					
29901267	10	33	theme	body	1158:1161	arg1	mass					1163:1166	lean body mass	1153:1166	lean body mass	1153:1166	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	7	34	theme	body	912:915	arg1	EE					917:918	whole body EE	906:918	whole body EE	906:918	These metabolic changes occurred without alterations in circulating insulin levels and despite increases in whole body EE.					
29901267	7	35	theme	metabolic	804:812	arg1	changes					814:820	These metabolic changes	798:820	These metabolic changes	798:820	These metabolic changes occurred without alterations in circulating insulin levels and despite increases in whole body EE.					
29901267	11	36	theme	Weight	1287:1292	arg1	gain					1294:1297	CONCLUSIONS Weight gain	1275:1297	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice	1275:1341	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	10	37	from	effects	1122:1128	arg1	EE					1133:1134	EE	1133:1134	EE	1133:1134	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	4	38	theme	C57BL/6J	484:491	arg1	mice					493:496	METHODS C57BL/6J mice	476:496	METHODS C57BL/6J mice	476:496	METHODS C57BL/6J mice were given 30% sucrose in liquid form.					
29901267	1	39	theme	OBJECTIVE	119:127	arg1	factors					138:144	OBJECTIVE Multiple factors	119:144	OBJECTIVE Multiple factors	119:144	OBJECTIVE Multiple factors contribute to the rising rates of obesity and to difficulties in weight reduction that exist in the worldwide population.					
29901267	1	40	theme	weight	211:216	arg1	reduction					218:226	weight reduction	211:226	weight reduction	211:226	OBJECTIVE Multiple factors contribute to the rising rates of obesity and to difficulties in weight reduction that exist in the worldwide population.					
29901267	6	41	contain	had	709:711	arg2	mass					725:728	greater fat mass	713:728	greater fat mass	713:728	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	6	41	contain	had	709:711	arg1	Mice					652:655	RESULTS Mice	644:655	RESULTS Mice drinking sucrose	644:672	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	10	42	theme	potential	1196:1204	arg1	covariates					1206:1215	potential covariates	1196:1215	potential covariates for EE	1196:1222	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	4	43	theme	METHODS	476:482	arg1	mice					493:496	METHODS C57BL/6J mice	476:496	METHODS C57BL/6J mice	476:496	METHODS C57BL/6J mice were given 30% sucrose in liquid form.					
29901267	11	44	theme	lipid	1362:1366	arg1	accrual					1368:1374	lipid accrual	1362:1374	lipid accrual in liver, but not skeletal muscle,	1362:1409	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	11	45	theme	CONCLUSIONS	1275:1285	arg1	gain					1294:1297	CONCLUSIONS Weight gain	1275:1297	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice	1275:1341	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	10	46	theme	treatment	1112:1120	arg1	effects					1122:1128	treatment effects	1112:1128	treatment effects on EE	1112:1134	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	0	47	theme	Glucose	56:62	arg1	Tolerance					64:72	Glucose Tolerance	56:72	Glucose Tolerance	56:72	Liquid Sucrose Consumption Promotes Obesity and Impairs Glucose Tolerance Without Altering Circulating Insulin Levels.					
29901267	11	48	theme	liquid	1320:1325	arg1	sucrose					1327:1333	liquid sucrose	1320:1333	liquid sucrose	1320:1333	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	11	49	from	accrual	1368:1374	arg1	muscle					1403:1408	skeletal muscle	1394:1408	skeletal muscle	1394:1408	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	11	49	from	accrual	1368:1374	arg1	liver					1379:1383	liver	1379:1383	liver	1379:1383	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	5	50	theme	body	561:564	arg1	composition					566:576	body composition	561:576	body composition	561:576	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	1	51	theme	Multiple	129:136	arg1	factors					138:144	OBJECTIVE Multiple factors	119:144	OBJECTIVE Multiple factors	119:144	OBJECTIVE Multiple factors contribute to the rising rates of obesity and to difficulties in weight reduction that exist in the worldwide population.					
29901267	3	52	theme	insulin	430:436	arg1	levels					438:443	serum insulin levels	424:443	serum insulin levels	424:443	This study tested the hypothesis that liquid sucrose intake promotes obesity by increasing serum insulin levels and tissue lipid accumulation.					
29901267	9	53	theme	fat	1091:1093	arg1	mass					1095:1098	fat mass	1091:1098	fat mass	1091:1098	Oxygen consumption (VO2 ) correlated with fat-free mass and moderately with TBM, but not with fat mass.					
29901267	11	54	theme	skeletal	1394:1401	arg1	muscle					1403:1408	skeletal muscle	1394:1408	skeletal muscle	1394:1408	CONCLUSIONS Weight gain induced by intake of liquid sucrose in mice is associated with lipid accrual in liver, but not skeletal muscle, and occurs without an increase in circulating insulin.					
29901267	8	55	theme	sucrose-consuming	973:989	arg1	mice					991:994	sucrose-consuming mice	973:994	sucrose-consuming mice	973:994	Lipid accrued in liver, but not skeletal muscle, of sucrose-consuming mice.					
29901267	10	56	with	ANCOVA	1101:1106	arg1	TBM					1142:1144	TBM	1142:1144	TBM	1142:1144	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	10	56	with	ANCOVA	1101:1106	arg1	VO2					1147:1149	VO2	1147:1149	VO2	1147:1149	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	10	56	with	ANCOVA	1101:1106	arg1	mass					1163:1166	lean body mass	1153:1166	lean body mass	1153:1166	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	10	56	with	ANCOVA	1101:1106	arg1	mass					1182:1185	fat-free mass	1173:1185	fat-free mass	1173:1185	ANCOVA for treatment effects on EE, with TBM, VO2 , lean body mass, and fat-free mass taken as potential covariates for EE, revealed VO2 as the most significant correlation.					
29901267	6	57	theme	fat	721:723	arg1	mass					725:728	greater fat mass	713:728	greater fat mass	713:728	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	8	58	theme	skeletal	953:960	arg1	muscle					962:967	skeletal muscle	953:967	skeletal muscle	953:967	Lipid accrued in liver, but not skeletal muscle, of sucrose-consuming mice.					
29901267	7	59	theme	whole	906:910	arg1	EE					917:918	whole body EE	906:918	whole body EE	906:918	These metabolic changes occurred without alterations in circulating insulin levels and despite increases in whole body EE.					
29901267	2	60	theme	Caloric	268:274	arg1	intake					276:281	Caloric intake	268:281	Caloric intake via sugar-sweetened beverages	268:311	Caloric intake via sugar-sweetened beverages may be influential.					
29901267	6	61	theme	greater	713:719	arg1	mass					725:728	greater fat mass	713:728	greater fat mass	713:728	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	6	62	theme	RESULTS	644:650	arg1	Mice					652:655	RESULTS Mice	644:655	RESULTS Mice drinking sucrose	644:672	RESULTS Mice drinking sucrose gained more total body mass (TBM), had greater fat mass, and displayed impaired glucose tolerance relative to control mice.					
29901267	7	63	from	alterations	839:849	arg1	levels					874:879	circulating insulin levels	854:879	circulating insulin levels	854:879	These metabolic changes occurred without alterations in circulating insulin levels and despite increases in whole body EE.					
29901267	1	64	from	difficulties	195:206	arg1	reduction					218:226	weight reduction	211:226	weight reduction	211:226	OBJECTIVE Multiple factors contribute to the rising rates of obesity and to difficulties in weight reduction that exist in the worldwide population.					
29901267	9	65	theme	Oxygen	997:1002	arg1	consumption					1004:1014	Oxygen consumption	997:1014	Oxygen consumption (VO2 )	997:1021	Oxygen consumption (VO2 ) correlated with fat-free mass and moderately with TBM, but not with fat mass.					
29901267	9	65	theme	Oxygen	997:1002	arg1	VO2					1017:1019	VO2	1017:1019	VO2	1017:1019	Oxygen consumption (VO2 ) correlated with fat-free mass and moderately with TBM, but not with fat mass.					
29901267	3	66	theme	serum	424:428	arg1	levels					438:443	serum insulin levels	424:443	serum insulin levels	424:443	This study tested the hypothesis that liquid sucrose intake promotes obesity by increasing serum insulin levels and tissue lipid accumulation.					
29901267	4	67	theme	liquid	524:529	arg1	form					531:534	liquid form	524:534	liquid form	524:534	METHODS C57BL/6J mice were given 30% sucrose in liquid form.					
29901267	5	68	theme	weight	548:553	arg1	gain					555:558	weight gain	548:558	weight gain	548:558	Changes in weight gain, body composition, energy expenditure (EE), and tissue lipid content were measured.					
29901267	1	69	theme	rising	164:169	arg1	rates					171:175	the rising rates	160:175	the rising rates of obesity	160:186	OBJECTIVE Multiple factors contribute to the rising rates of obesity and to difficulties in weight reduction that exist in the worldwide population.					
29901267	1	70	theme	worldwide	246:254	arg1	population					256:265	the worldwide population	242:265	the worldwide population	242:265	OBJECTIVE Multiple factors contribute to the rising rates of obesity and to difficulties in weight reduction that exist in the worldwide population.					
30894435	4	0	theme	human	692:696	arg1	oligosaccharides					698:713	human oligosaccharides	692:713	human oligosaccharides	692:713	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	5	1	from	Differences	716:726	arg1	proportions					735:745	the proportions	731:745	the proportions of the two genera	731:763	Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations.					
30894435	6	2	from	differences	945:955	arg1	terms					960:964	terms	960:964	terms of the taxonomic annotation	960:992	Our results show that differences in terms of the taxonomic annotation do not explain the reason for the differences in the Bifidobacterium/Bacteroides ratio between the Finnish and the Russian populations.					
30894435	4	3	theme	gut-colonizing	513:526	arg1	strains					528:534	gut-colonizing strains	513:534	gut-colonizing strains	513:534	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	4	3	theme	gut-colonizing	513:526	arg1	Bacteroides					565:575	Bacteroides	565:575	Bacteroides	565:575	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	4	3	theme	gut-colonizing	513:526	arg1	Bifidobacterium					545:559	Bifidobacterium	545:559	Bifidobacterium	545:559	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	9	4	theme	emerging	1559:1566	arg1	need					1568:1571	an emerging need	1556:1571	an emerging need for the development of higher-resolution techniques	1556:1623	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	1	5	theme	Western	274:280	arg1	countries					282:290	Western countries	274:290	Western countries	274:290	A variety of autoimmune and allergy events are becoming increasingly common, especially in Western countries.					
30894435	0	6	theme	Function-Level	116:129	arg1	Adaptation					131:140	Function-Level Adaptation	116:140	Function-Level Adaptation to Human Milk in the Finnish Population	116:180	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	11	7	theme	differences	1838:1848	arg1	detection					1825:1833	the detection	1821:1833	the detection of differences between functionalities present in metagenomic samples	1821:1903	BiomeScout-the presented technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.					
30894435	2	8	theme	research	308:315	arg1	pieces					298:303	Some pieces	293:303	Some pieces of research	293:315	Some pieces of research link such conditions with the composition of microbiota during infancy.					
30894435	0	9	from	Population	171:180	arg1	Depletion					103:111	Depletion	103:111	Depletion of Function-Level Adaptation to Human Milk in the Finnish Population	103:180	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	7	10	theme	analysis	1186:1193	arg1	results					1160:1166	the results	1156:1166	the results of function-level analysis	1156:1193	In this paper, we present the results of function-level analysis.					
30894435	10	11	theme	novel	1683:1687	arg1	method					1689:1694	the novel method	1679:1694	the novel method used for the analysis of function-level metagenomes	1679:1746	The significance of this research is demonstrated by the novel method used for the analysis of function-level metagenomes.					
30894435	9	12	theme	oligosaccharides.IMPORTANCE	1467:1493	arg1	utilization					1439:1449	the utilization	1435:1449	the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques	1435:1623	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	10	13	theme	function-level	1721:1734	arg1	metagenomes					1736:1746	function-level metagenomes	1721:1746	function-level metagenomes	1721:1746	The significance of this research is demonstrated by the novel method used for the analysis of function-level metagenomes.					
30894435	7	14	theme	function-level	1171:1184	arg1	analysis					1186:1193	function-level analysis	1171:1193	function-level analysis	1171:1193	In this paper, we present the results of function-level analysis.					
30894435	9	15	from	differences	1365:1375	arg1	abundances					1384:1393	the abundances	1380:1393	the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques	1380:1623	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	8	16	theme	BiomeScout	1253:1262	arg1	technology					1264:1273	BiomeScout technology	1253:1273	BiomeScout technology	1253:1273	Unlike the typical workflow for gene abundance analysis, BiomeScout technology explains the differences in the Bifidobacterium/Bacteroides ratio.					
30894435	5	17	theme	autoimmune	859:868	arg1	diseases					870:877	autoimmune diseases	859:877	autoimmune diseases	859:877	Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations.					
30894435	5	18	theme	allergies	845:853	arg1	frequency					832:840	the frequency	828:840	the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations	828:920	Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations.					
30894435	0	19	theme	Adaptation	131:140	arg1	Depletion					103:111	Depletion	103:111	Depletion of Function-Level Adaptation to Human Milk in the Finnish Population	103:180	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	10	20	theme	metagenomes	1736:1746	arg1	analysis					1709:1716	the analysis	1705:1716	the analysis of function-level metagenomes	1705:1746	The significance of this research is demonstrated by the novel method used for the analysis of function-level metagenomes.					
30894435	9	21	theme	research	1537:1544	arg1	limitations					1507:1517	the limitations	1503:1517	the limitations of taxonomy-based research	1503:1544	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	11	22	theme	present	1874:1880	arg1	functionalities					1858:1872	functionalities	1858:1872	functionalities present in metagenomic samples	1858:1903	BiomeScout-the presented technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.					
30894435	8	23	theme	gene	1228:1231	arg1	analysis					1243:1250	gene abundance analysis	1228:1250	gene abundance analysis	1228:1250	Unlike the typical workflow for gene abundance analysis, BiomeScout technology explains the differences in the Bifidobacterium/Bacteroides ratio.					
30894435	8	24	from	differences	1288:1298	arg1	ratio					1335:1339	the Bifidobacterium/Bacteroides ratio	1303:1339	the Bifidobacterium/Bacteroides ratio	1303:1339	Unlike the typical workflow for gene abundance analysis, BiomeScout technology explains the differences in the Bifidobacterium/Bacteroides ratio.					
30894435	11	25	theme	metagenomic	1885:1895	arg1	samples					1897:1903	metagenomic samples	1885:1903	metagenomic samples	1885:1903	BiomeScout-the presented technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.					
30894435	11	26	from	samples	1897:1903	arg1	present					1874:1880	present	1874:1880	present	1874:1880	BiomeScout-the presented technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.					
30894435	5	27	dep	Finnish	886:892	arg1	populations					910:920	populations	910:920	populations	910:920	Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations.					
30894435	5	27	dep	Finnish	886:892	arg1	the					882:884	the	882:884	the	882:884	Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations.					
30894435	1	28	theme	autoimmune	196:205	arg1	events					219:224	autoimmune and allergy events	196:224	autoimmune and allergy events	196:224	A variety of autoimmune and allergy events are becoming increasingly common, especially in Western countries.					
30894435	10	29	theme	research	1651:1658	arg1	significance					1630:1641	The significance	1626:1641	The significance of this research	1626:1658	The significance of this research is demonstrated by the novel method used for the analysis of function-level metagenomes.					
30894435	8	30	theme	abundance	1233:1241	arg1	analysis					1243:1250	gene abundance analysis	1228:1250	gene abundance analysis	1228:1250	Unlike the typical workflow for gene abundance analysis, BiomeScout technology explains the differences in the Bifidobacterium/Bacteroides ratio.					
30894435	9	31	theme	higher-resolution	1596:1612	arg1	techniques					1614:1623	higher-resolution techniques	1596:1623	higher-resolution techniques	1596:1623	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	3	32	theme	gut	443:445	arg1	microbiota					447:456	gut microbiota	443:456	gut microbiota	443:456	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	0	33	theme	Metabolic	34:42	arg1	Pathways					44:51	Differentiating Metabolic Pathways	18:51	Differentiating Metabolic Pathways	18:51	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	6	34	from	differences	1028:1038	arg1	ratio					1075:1079	the Bifidobacterium/Bacteroides ratio	1043:1079	the Bifidobacterium/Bacteroides ratio between the Finnish and the Russian populations	1043:1127	Our results show that differences in terms of the taxonomic annotation do not explain the reason for the differences in the Bifidobacterium/Bacteroides ratio between the Finnish and the Russian populations.					
30894435	2	35	theme	such	322:325	arg1	conditions					327:336	such conditions	322:336	such conditions	322:336	Some pieces of research link such conditions with the composition of microbiota during infancy.					
30894435	1	36	theme	allergy	211:217	arg1	events					219:224	autoimmune and allergy events	196:224	autoimmune and allergy events	196:224	A variety of autoimmune and allergy events are becoming increasingly common, especially in Western countries.					
30894435	0	37	theme	Differentiating	18:32	arg1	Pathways					44:51	Differentiating Metabolic Pathways	18:51	Differentiating Metabolic Pathways	18:51	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	0	38	theme	Human	145:149	arg1	Milk					151:154	Human Milk	145:154	Human Milk	145:154	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	0	39	from	Depletion	103:111	arg1	Population					171:180	the Finnish Population	159:180	the Finnish Population	159:180	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	1	40	theme	events	219:224	arg1	events					219:224	autoimmune and allergy events	196:224	autoimmune and allergy events	196:224	A variety of autoimmune and allergy events are becoming increasingly common, especially in Western countries.					
30894435	1	40	theme	events	219:224	arg1	variety					185:191	A variety	183:191	A variety of autoimmune and allergy events	183:224	A variety of autoimmune and allergy events are becoming increasingly common, especially in Western countries.					
30894435	8	41	theme	typical	1207:1213	arg1	workflow					1215:1222	the typical workflow	1203:1222	the typical workflow for gene abundance analysis	1203:1250	Unlike the typical workflow for gene abundance analysis, BiomeScout technology explains the differences in the Bifidobacterium/Bacteroides ratio.					
30894435	9	42	theme	taxonomy-based	1522:1535	arg1	research					1537:1544	taxonomy-based research	1522:1544	taxonomy-based research	1522:1544	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	6	43	dep	Finnish	1093:1099	arg1	populations					1117:1127	populations	1117:1127	populations	1117:1127	Our results show that differences in terms of the taxonomic annotation do not explain the reason for the differences in the Bifidobacterium/Bacteroides ratio between the Finnish and the Russian populations.					
30894435	5	44	theme	diseases	870:877	arg1	frequency					832:840	the frequency	828:840	the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations	828:920	Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations.					
30894435	0	45	theme	Pathways	44:51	arg1	Identification					0:13	Identification	0:13	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations	0:93	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	3	46	theme	nutrition	429:437	arg1	form					421:424	the predominant form	405:424	the predominant form of nutrition for gut microbiota	405:456	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	3	46	theme	nutrition	429:437	arg1	oligosaccharides					461:476	oligosaccharides	461:476	oligosaccharides from human milk (HMO)	461:498	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	6	47	theme	annotation	983:992	arg1	terms					960:964	terms	960:964	terms of the taxonomic annotation	960:992	Our results show that differences in terms of the taxonomic annotation do not explain the reason for the differences in the Bifidobacterium/Bacteroides ratio between the Finnish and the Russian populations.					
30894435	3	48	from	oligosaccharides	461:476	arg1	period					397:402	this period	392:402	this period	392:402	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	6	49	theme	Bifidobacterium/Bacteroides	1047:1073	arg1	ratio					1075:1079	the Bifidobacterium/Bacteroides ratio	1043:1079	the Bifidobacterium/Bacteroides ratio between the Finnish and the Russian populations	1043:1127	Our results show that differences in terms of the taxonomic annotation do not explain the reason for the differences in the Bifidobacterium/Bacteroides ratio between the Finnish and the Russian populations.					
30894435	0	50	theme	Gut	68:70	arg1	Populations					83:93	Infant Gut Microbiome Populations	61:93	Infant Gut Microbiome Populations	61:93	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	4	51	theme	strains	528:534	arg1	number					503:508	A number	501:508	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides,	501:576	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	4	51	theme	strains	528:534	arg1	Bacteroides					565:575	Bacteroides	565:575	Bacteroides	565:575	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	4	51	theme	strains	528:534	arg1	able					582:585	able	582:585	able	582:585	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	4	51	theme	strains	528:534	arg1	Bifidobacterium					545:559	Bifidobacterium	545:559	Bifidobacterium	545:559	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	5	52	theme	genera	758:763	arg1	proportions					735:745	the proportions	731:745	the proportions of the two genera	731:763	Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations.					
30894435	2	53	theme	microbiota	362:371	arg1	composition					347:357	the composition	343:357	the composition of microbiota during infancy	343:386	Some pieces of research link such conditions with the composition of microbiota during infancy.					
30894435	0	54	theme	Infant	61:66	arg1	Populations					83:93	Infant Gut Microbiome Populations	61:93	Infant Gut Microbiome Populations	61:93	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	0	55	theme	Finnish	163:169	arg1	Population					171:180	the Finnish Population	159:180	the Finnish Population	159:180	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	11	56	theme	proprietary	1794:1804	arg1	algorithms					1806:1815	technology-utilizes proprietary algorithms	1774:1815	technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples	1774:1903	BiomeScout-the presented technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.					
30894435	9	57	dep	utilization	1439:1449	arg1	is					1553:1554	is	1553:1554	is an emerging need for the development of higher-resolution techniques	1553:1623	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	4	58	theme	oligosaccharides	698:713	arg1	composition					677:687	the specific composition	664:687	the specific composition of human oligosaccharides	664:713	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	7	59	attach	present	1148:1154	arg2	we					1145:1146	we	1145:1146	we	1145:1146	In this paper, we present the results of function-level analysis.					
30894435	7	59	attach	present	1148:1154	arg1	paper					1138:1142	this paper	1133:1142	this paper	1133:1142	In this paper, we present the results of function-level analysis.					
30894435	0	60	from	Adaptation	131:140	arg1	Population					171:180	the Finnish Population	159:180	the Finnish Population	159:180	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	3	61	theme	predominant	409:419	arg1	form					421:424	the predominant form	405:424	the predominant form of nutrition for gut microbiota	405:456	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	3	61	theme	predominant	409:419	arg1	oligosaccharides					461:476	oligosaccharides	461:476	oligosaccharides from human milk (HMO)	461:498	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	3	62	from	milk	489:492	arg1	form					421:424	the predominant form	405:424	the predominant form of nutrition for gut microbiota	405:456	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	3	62	from	milk	489:492	arg1	oligosaccharides					461:476	oligosaccharides	461:476	oligosaccharides from human milk (HMO)	461:498	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	8	63	theme	Bifidobacterium/Bacteroides	1307:1333	arg1	ratio					1335:1339	the Bifidobacterium/Bacteroides ratio	1303:1339	the Bifidobacterium/Bacteroides ratio	1303:1339	Unlike the typical workflow for gene abundance analysis, BiomeScout technology explains the differences in the Bifidobacterium/Bacteroides ratio.					
30894435	9	64	theme	techniques	1614:1623	arg1	development					1581:1591	the development	1577:1591	the development of higher-resolution techniques	1577:1623	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	6	65	theme	taxonomic	973:981	arg1	annotation					983:992	the taxonomic annotation	969:992	the taxonomic annotation	969:992	Our results show that differences in terms of the taxonomic annotation do not explain the reason for the differences in the Bifidobacterium/Bacteroides ratio between the Finnish and the Russian populations.					
30894435	5	66	from	frequency	832:840	arg1	Finnish					886:892	Finnish	886:892	Finnish	886:892	Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations.					
30894435	5	66	from	frequency	832:840	arg1	Russian					902:908	Russian	902:908	Russian	902:908	Differences in the proportions of the two genera that are able to utilize HMO have already been associated with the frequency of allergies and autoimmune diseases in the Finnish and the Russian populations.					
30894435	9	67	theme	type	1460:1463	arg1	oligosaccharides.IMPORTANCE					1467:1493	short type 1 oligosaccharides.IMPORTANCE	1454:1493	short type 1 oligosaccharides.IMPORTANCE	1454:1493	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	9	68	theme	enzymes	1406:1412	arg1	abundances					1384:1393	the abundances	1380:1393	the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques	1380:1623	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	11	69	from	present	1874:1880	arg1	samples					1897:1903	metagenomic samples	1885:1903	metagenomic samples	1885:1903	BiomeScout-the presented technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.					
30894435	11	70	theme	technology-utilizes	1774:1792	arg1	algorithms					1806:1815	technology-utilizes proprietary algorithms	1774:1815	technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples	1774:1903	BiomeScout-the presented technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.					
30894435	11	71	attach	present	1874:1880	arg1	samples					1897:1903	metagenomic samples	1885:1903	metagenomic samples	1885:1903	BiomeScout-the presented technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.					
30894435	11	71	attach	present	1874:1880	arg2	functionalities					1858:1872	functionalities	1858:1872	functionalities present in metagenomic samples	1858:1903	BiomeScout-the presented technology-utilizes proprietary algorithms for the detection of differences between functionalities present in metagenomic samples.					
30894435	3	72	theme	human	483:487	arg1	HMO					495:497	HMO	495:497	HMO	495:497	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	3	72	theme	human	483:487	arg1	milk					489:492	human milk	483:492	human milk (HMO)	483:498	In this period, the predominant form of nutrition for gut microbiota is oligosaccharides from human milk (HMO).					
30894435	9	73	theme	short	1454:1458	arg1	oligosaccharides.IMPORTANCE					1467:1493	short type 1 oligosaccharides.IMPORTANCE	1454:1493	short type 1 oligosaccharides.IMPORTANCE	1454:1493	Our research shows the differences in the abundances of the two enzymes that are crucial for the utilization of short type 1 oligosaccharides.IMPORTANCE Knowing the limitations of taxonomy-based research, there is an emerging need for the development of higher-resolution techniques.					
30894435	0	74	theme	Microbiome	72:81	arg1	Populations					83:93	Infant Gut Microbiome Populations	61:93	Infant Gut Microbiome Populations	61:93	Identification of Differentiating Metabolic Pathways between Infant Gut Microbiome Populations Reveals Depletion of Function-Level Adaptation to Human Milk in the Finnish Population.					
30894435	4	75	theme	Bifidobacterium	617:631	arg1	strains					633:639	only some Bifidobacterium strains	607:639	only some Bifidobacterium strains	607:639	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
30894435	4	76	theme	specific	668:675	arg1	composition					677:687	the specific composition	664:687	the specific composition of human oligosaccharides	664:713	A number of gut-colonizing strains, such as Bifidobacterium and Bacteroides, are able to utilize HMO, but only some Bifidobacterium strains have evolved to digest the specific composition of human oligosaccharides.					
31240496	4	0	from	PC	552:553	arg1	number					529:534	Absolute number	520:534	Absolute number of cells in the PC	520:553	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	4	0	from	PC	552:553	arg1	markers					635:641	CD24 markers	630:641	CD24 markers	630:641	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	4	0	from	PC	552:553	arg1	composition					574:584	the subpopulation composition	556:584	the subpopulation composition of tumor with flow cytometry using CD44	556:624	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	6	1	theme	EC	915:916	arg1	composition					932:942	EC subpopulation composition	915:942	EC subpopulation composition	915:942	Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used.					
31240496	6	2	from	decrease	961:968	arg1	intensity					990:998	the tumor growth intensity	973:998	the tumor growth intensity	973:998	Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used.					
31240496	9	3	theme	EC	1350:1351	arg1	composition					1331:1341	the subpopulation composition	1313:1341	the subpopulation composition of the EC and intensity of its growth in vivo	1313:1387	The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.					
31240496	7	4	with	inhibition	1069:1078	arg1	number					1111:1116	a reduced number	1101:1116	a reduced number of CD44+ and increased CD24+ cells	1101:1151	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	7	5	theme	increased	1131:1139	arg1	cells					1147:1151	CD44+ and increased CD24+ cells	1121:1151	CD44+ and increased CD24+ cells	1121:1151	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	5	6	theme	EC	719:720	arg1	composition					736:746	EC subpopulation composition	719:746	EC subpopulation composition	719:746	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	1	7	theme	low	150:152	arg1	temperatures					154:165	low temperatures	150:165	low temperatures	150:165	The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells.					
31240496	7	8	theme	CD24+	1141:1145	arg1	cells					1147:1151	CD44+ and increased CD24+ cells	1121:1151	CD44+ and increased CD24+ cells	1121:1151	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	0	9	from	Effect	0:5	arg1	growth					67:72	Ehrlich carcinoma growth	49:72	Ehrlich carcinoma growth	49:72	Effect of different cryopreservation regimens on Ehrlich carcinoma growth.					
31240496	2	10	theme	different	266:274	arg1	regimens					293:300	different cryopreservation regimens	266:300	different cryopreservation regimens	266:300	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	9	11	theme	growth	1374:1379	arg1	intensity					1357:1365	intensity	1357:1365	intensity	1357:1365	The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.					
31240496	9	11	theme	growth	1374:1379	arg1	EC					1350:1351	EC	1350:1351	EC	1350:1351	The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.					
31240496	4	12	from	cells	539:543	arg1	PC					552:553	the PC	548:553	the PC	548:553	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	7	13	theme	significant	1057:1067	arg1	inhibition					1069:1078	significant inhibition	1057:1078	significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells	1057:1151	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	3	14	theme	mice	503:506	arg1	PC					496:497	PC	496:497	PC	496:497	The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days.					
31240496	3	14	theme	mice	503:506	arg1	cavity					488:493	peritoneal cavity	477:493	peritoneal cavity (PC) of mice for 7 days	477:517	The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days.					
31240496	4	15	theme	cells	539:543	arg1	number					529:534	Absolute number	520:534	Absolute number of cells in the PC	520:553	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	4	15	theme	cells	539:543	arg1	markers					635:641	CD24 markers	630:641	CD24 markers	630:641	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	4	15	theme	cells	539:543	arg1	composition					574:584	the subpopulation composition	556:584	the subpopulation composition of tumor with flow cytometry using CD44	556:624	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	4	16	theme	CD24	630:633	arg1	markers					635:641	CD24 markers	630:641	CD24 markers	630:641	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	4	17	theme	tumor	589:593	arg1	number					529:534	Absolute number	520:534	Absolute number of cells in the PC	520:553	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	4	17	theme	tumor	589:593	arg1	markers					635:641	CD24 markers	630:641	CD24 markers	630:641	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	4	17	theme	tumor	589:593	arg1	composition					574:584	the subpopulation composition	556:584	the subpopulation composition of tumor with flow cytometry using CD44	556:624	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	5	18	theme	tumorigenic	785:795	arg1	cells					806:810	the most tumorigenic CD44high cells	776:810	the most tumorigenic CD44high cells after both freezing regimens	776:839	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	4	19	theme	subpopulation	560:572	arg1	composition					574:584	the subpopulation composition	556:584	the subpopulation composition of tumor with flow cytometry using CD44	556:624	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	8	20	theme	complete	1206:1213	arg1	elimination					1215:1225	a complete elimination	1204:1225	a complete elimination of tumorigenic CD44high tumor cells	1204:1261	None of the cryopreservation regimens resulted in a complete elimination of tumorigenic CD44high tumor cells.					
31240496	5	21	theme	freezing	823:830	arg1	regimens					832:839	both freezing regimens	818:839	both freezing regimens	818:839	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	3	22	theme	cryopreserved	411:423	arg1	cells					454:458	The previously cryopreserved with slow and rapid rates EC cells	396:458	The previously cryopreserved with slow and rapid rates EC cells	396:458	The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days.					
31240496	4	23	from	number	529:534	arg1	PC					552:553	the PC	548:553	the PC	548:553	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	9	24	theme	freezing	1268:1275	arg1	rate					1277:1280	The freezing rate	1264:1280	The freezing rate	1264:1280	The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.					
31240496	9	25	dep	EC	1350:1351	arg1	the					1346:1348	the	1346:1348	the	1346:1348	The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.					
31240496	1	26	theme	freezing	79:86	arg1	factor					107:112	a decisive factor	96:112	a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells	96:219	The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells.					
31240496	1	26	theme	freezing	79:86	arg1	rate					88:91	The freezing rate	75:91	The freezing rate	75:91	The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells.					
31240496	8	27	theme	cryopreservation	1166:1181	arg1	regimens					1183:1190	the cryopreservation regimens	1162:1190	the cryopreservation regimens	1162:1190	None of the cryopreservation regimens resulted in a complete elimination of tumorigenic CD44high tumor cells.					
31240496	5	28	theme	decreased	755:763	arg1	content					765:771	a decreased content	753:771	a decreased content of the most tumorigenic CD44high cells after both freezing regimens	753:839	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	2	29	theme	subpopulation	347:359	arg1	composition					361:371	subpopulation composition	347:371	subpopulation composition	347:371	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	7	30	theme	CD44+	1121:1125	arg1	cells					1147:1151	CD44+ and increased CD24+ cells	1121:1151	CD44+ and increased CD24+ cells	1121:1151	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	6	31	theme	composition	932:942	arg1	restoration					900:910	the restoration	896:910	the restoration of EC subpopulation composition	896:942	Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used.					
31240496	7	32	theme	Rapid	1028:1032	arg1	cooling					1034:1040	Rapid cooling	1028:1040	Rapid cooling	1028:1040	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	4	33	theme	flow	600:603	arg1	cytometry					605:613	flow cytometry	600:613	flow cytometry using CD44	600:624	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	2	34	theme	regimens	293:300	arg1	effect					256:261	effect	256:261	effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites	256:393	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	6	35	theme	slow	1005:1008	arg1	cooling					1010:1016	slow cooling	1005:1016	slow cooling	1005:1016	Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used.					
31240496	0	36	theme	cryopreservation	20:35	arg1	regimens					37:44	different cryopreservation regimens	10:44	different cryopreservation regimens	10:44	Effect of different cryopreservation regimens on Ehrlich carcinoma growth.					
31240496	5	37	theme	CD44high	797:804	arg1	cells					806:810	the most tumorigenic CD44high cells	776:810	the most tumorigenic CD44high cells after both freezing regimens	776:839	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	8	38	theme	tumor	1251:1255	arg1	cells					1257:1261	tumorigenic CD44high tumor cells	1230:1261	tumorigenic CD44high tumor cells	1230:1261	None of the cryopreservation regimens resulted in a complete elimination of tumorigenic CD44high tumor cells.					
31240496	6	39	used	used	1022:1025	arg2	cooling					1010:1016	slow cooling	1005:1016	slow cooling	1005:1016	Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used.					
31240496	2	40	theme	cryopreservation	276:291	arg1	regimens					293:300	different cryopreservation regimens	266:300	different cryopreservation regimens	266:300	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	3	41	theme	rapid	439:443	arg1	rates					445:449	slow and rapid rates	430:449	slow and rapid rates	430:449	The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days.					
31240496	0	42	theme	different	10:18	arg1	regimens					37:44	different cryopreservation regimens	10:44	different cryopreservation regimens	10:44	Effect of different cryopreservation regimens on Ehrlich carcinoma growth.					
31240496	4	43	theme	Absolute	520:527	arg1	number					529:534	Absolute number	520:534	Absolute number of cells in the PC	520:553	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	1	44	theme	decisive	98:105	arg1	factor					107:112	a decisive factor	96:112	a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells	96:219	The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells.					
31240496	1	44	theme	decisive	98:105	arg1	rate					88:91	The freezing rate	75:91	The freezing rate	75:91	The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells.					
31240496	4	45	from	composition	574:584	arg1	PC					552:553	the PC	548:553	the PC	548:553	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	2	46	theme	ascites	387:393	arg1	composition					361:371	subpopulation composition	347:371	subpopulation composition	347:371	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	2	46	theme	ascites	387:393	arg1	growth					328:333	Ehrlich carcinoma (EC) growth	305:333	Ehrlich carcinoma (EC) growth in vivo	305:341	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	5	47	theme	subpopulation	722:734	arg1	composition					736:746	EC subpopulation composition	719:746	EC subpopulation composition	719:746	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	3	48	theme	slow	430:433	arg1	rates					445:449	slow and rapid rates	430:449	slow and rapid rates	430:449	The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days.					
31240496	9	49	theme	intensity	1357:1365	arg1	composition					1331:1341	the subpopulation composition	1313:1341	the subpopulation composition of the EC and intensity of its growth in vivo	1313:1387	The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.					
31240496	8	50	theme	cells	1257:1261	arg1	elimination					1215:1225	a complete elimination	1204:1225	a complete elimination of tumorigenic CD44high tumor cells	1204:1261	None of the cryopreservation regimens resulted in a complete elimination of tumorigenic CD44high tumor cells.					
31240496	2	51	theme	formed	380:385	arg1	ascites					387:393	the formed ascites	376:393	the formed ascites	376:393	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	0	52	theme	regimens	37:44	arg1	Effect					0:5	Effect	0:5	Effect of different cryopreservation regimens on Ehrlich carcinoma growth.	0:73	Effect of different cryopreservation regimens on Ehrlich carcinoma growth.					
31240496	5	53	theme	composition	736:746	arg1	redistribution					701:714	a significant redistribution	687:714	a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens	687:839	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	7	54	theme	reduced	1103:1109	arg1	number					1111:1116	a reduced number	1101:1116	a reduced number of CD44+ and increased CD24+ cells	1101:1151	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	5	55	theme	cells	806:810	arg1	content					765:771	a decreased content	753:771	a decreased content of the most tumorigenic CD44high cells after both freezing regimens	753:839	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	0	56	theme	carcinoma	57:65	arg1	growth					67:72	Ehrlich carcinoma growth	49:72	Ehrlich carcinoma growth	49:72	Effect of different cryopreservation regimens on Ehrlich carcinoma growth.					
31240496	9	57	theme	subpopulation	1317:1329	arg1	composition					1331:1341	the subpopulation composition	1313:1341	the subpopulation composition of the EC and intensity of its growth in vivo	1313:1387	The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.					
31240496	6	58	theme	subpopulation	918:930	arg1	composition					932:942	EC subpopulation composition	915:942	EC subpopulation composition	915:942	Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used.					
31240496	8	59	theme	CD44high	1242:1249	arg1	cells					1257:1261	tumorigenic CD44high tumor cells	1230:1261	tumorigenic CD44high tumor cells	1230:1261	None of the cryopreservation regimens resulted in a complete elimination of tumorigenic CD44high tumor cells.					
31240496	0	60	theme	Ehrlich	49:55	arg1	growth					67:72	Ehrlich carcinoma growth	49:72	Ehrlich carcinoma growth	49:72	Effect of different cryopreservation regimens on Ehrlich carcinoma growth.					
31240496	9	61	theme	composition	1331:1341	arg1	preservation					1297:1308	the preservation	1293:1308	the preservation of the subpopulation composition of the EC and intensity of its growth in vivo	1293:1387	The freezing rate determines the preservation of the subpopulation composition of the EC and intensity of its growth in vivo.					
31240496	3	62	theme	EC	451:452	arg1	cells					454:458	The previously cryopreserved with slow and rapid rates EC cells	396:458	The previously cryopreserved with slow and rapid rates EC cells	396:458	The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days.					
31240496	4	63	with	tumor	589:593	arg1	cytometry					605:613	flow cytometry	600:613	flow cytometry using CD44	600:624	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	3	64	theme	peritoneal	477:486	arg1	PC					496:497	PC	496:497	PC	496:497	The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days.					
31240496	3	64	theme	peritoneal	477:486	arg1	cavity					488:493	peritoneal cavity	477:493	peritoneal cavity (PC) of mice for 7 days	477:517	The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days.					
31240496	8	65	theme	tumorigenic	1230:1240	arg1	cells					1257:1261	tumorigenic CD44high tumor cells	1230:1261	tumorigenic CD44high tumor cells	1230:1261	None of the cryopreservation regimens resulted in a complete elimination of tumorigenic CD44high tumor cells.					
31240496	3	66	with	cryopreserved	411:423	arg1	rates					445:449	slow and rapid rates	430:449	slow and rapid rates	430:449	The previously cryopreserved with slow and rapid rates EC cells were cultured in peritoneal cavity (PC) of mice for 7 days.					
31240496	7	67	theme	growth	1089:1094	arg1	inhibition					1069:1078	significant inhibition	1057:1078	significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells	1057:1151	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	2	68	theme	carcinoma	313:321	arg1	growth					328:333	Ehrlich carcinoma (EC) growth	305:333	Ehrlich carcinoma (EC) growth in vivo	305:341	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	7	69	theme	cells	1147:1151	arg1	number					1111:1116	a reduced number	1101:1116	a reduced number of CD44+ and increased CD24+ cells	1101:1151	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	2	70	theme	Ehrlich	305:311	arg1	EC					324:325	EC	324:325	EC	324:325	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	2	70	theme	Ehrlich	305:311	arg1	carcinoma					313:321	Ehrlich carcinoma	305:321	Ehrlich carcinoma (EC) growth in vivo	305:341	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	1	71	theme	tumor	209:213	arg1	cells					215:219	tumor cells	209:219	tumor cells	209:219	The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells.					
31240496	5	72	theme	significant	689:699	arg1	redistribution					701:714	a significant redistribution	687:714	a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens	687:839	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	6	73	theme	tumor	977:981	arg1	intensity					990:998	the tumor growth intensity	973:998	the tumor growth intensity	973:998	Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used.					
31240496	2	74	theme	research	226:233	arg1	aim					235:237	The research aim	222:237	The research aim	222:237	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	5	75	theme	most	780:783	arg1	cells					806:810	the most tumorigenic CD44high cells	776:810	the most tumorigenic CD44high cells after both freezing regimens	776:839	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	2	76	from	effect	256:261	arg1	composition					361:371	subpopulation composition	347:371	subpopulation composition	347:371	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	2	76	from	effect	256:261	arg1	growth					328:333	Ehrlich carcinoma (EC) growth	305:333	Ehrlich carcinoma (EC) growth in vivo	305:341	The research aim was to determine effect of different cryopreservation regimens on Ehrlich carcinoma (EC) growth in vivo and subpopulation composition of the formed ascites.					
31240496	1	77	theme	cells	215:219	arg1	cryoablation					178:189	cryoablation	178:189	cryoablation	178:189	The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells.					
31240496	1	77	theme	cells	215:219	arg1	cryobanking					194:204	cryobanking	194:204	cryobanking	194:204	The freezing rate is a decisive factor in determining the purpose of using low temperatures, i.e., for cryoablation or cryobanking of tumor cells.					
31240496	7	78	theme	tumor	1083:1087	arg1	growth					1089:1094	tumor growth	1083:1094	tumor growth	1083:1094	Rapid cooling contributed to significant inhibition of tumor growth with a reduced number of CD44+ and increased CD24+ cells.					
31240496	5	79	with	redistribution	701:714	arg1	content					765:771	a decreased content	753:771	a decreased content of the most tumorigenic CD44high cells after both freezing regimens	753:839	Immediately after warming, a significant redistribution of EC subpopulation composition with a decreased content of the most tumorigenic CD44high cells after both freezing regimens was found.					
31240496	8	80	theme	regimens	1183:1190	arg1	None					1154:1157	None	1154:1157	None of the cryopreservation regimens	1154:1190	None of the cryopreservation regimens resulted in a complete elimination of tumorigenic CD44high tumor cells.					
31240496	4	81	from	markers	635:641	arg1	PC					552:553	the PC	548:553	the PC	548:553	Absolute number of cells in the PC, the subpopulation composition of tumor with flow cytometry using CD44 and CD24 markers were determined.					
31240496	6	82	theme	growth	983:988	arg1	intensity					990:998	the tumor growth intensity	973:998	the tumor growth intensity	973:998	Culturing in vivo for 7 days contributed to the restoration of EC subpopulation composition, but with some a decrease in the tumor growth intensity when slow cooling was used.					
28972200	10	0	theme	alginate	1710:1717	arg1	sponges					1719:1725	thrombin-modified alginate sponges	1692:1725	thrombin-modified alginate sponges	1692:1725	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	10	0	theme	alginate	1710:1717	arg1	material					1766:1773	a grafting material	1755:1773	a grafting material by itself	1755:1783	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	9	1	theme	microvascular	1627:1639	arg1	network					1641:1647	microvascular network	1627:1647	microvascular network	1627:1647	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	6	2	theme	stromal	1213:1219	arg1	cells					1221:1225	subcutaneous adipose-derived stromal cells	1184:1225	subcutaneous adipose-derived stromal cells	1184:1225	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	4	3	theme	thrombin-modified	570:586	arg1	scaffolds					594:602	The novel thrombin-modified AG-FG scaffolds	560:602	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure	560:646	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	3	4	theme	Mg2+	522:525	arg1	cations					527:533	Ca2+ and Mg2+ cations	513:533	Ca2+ and Mg2+ cations	513:533	The slow rates of scaffold biodegradation were achieved by using Ca2+ and Mg2+ cations as cross-linking agents.					
28972200	3	4	theme	Mg2+	522:525	arg1	agents					552:557	cross-linking agents	538:557	cross-linking agents	538:557	The slow rates of scaffold biodegradation were achieved by using Ca2+ and Mg2+ cations as cross-linking agents.					
28972200	6	5	theme	subcutaneous	1184:1195	arg1	cells					1221:1225	subcutaneous adipose-derived stromal cells	1184:1225	subcutaneous adipose-derived stromal cells	1184:1225	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	2	6	used	used	412:415	arg2	εAc					403:405	εAc	403:405	εAc	403:405	Thrombin was added to drive FG/fibrin conversion, while ε-aminocapronic acid (εAc) was used as antifibrinolytic component.					
28972200	2	6	used	used	412:415	arg2	acid					397:400	ε-aminocapronic acid	381:400	ε-aminocapronic acid (εAc)	381:406	Thrombin was added to drive FG/fibrin conversion, while ε-aminocapronic acid (εAc) was used as antifibrinolytic component.					
28972200	2	6	used	used	412:415	arg2	component					437:445	antifibrinolytic component	420:445	antifibrinolytic component	420:445	Thrombin was added to drive FG/fibrin conversion, while ε-aminocapronic acid (εAc) was used as antifibrinolytic component.					
28972200	3	7	theme	Ca2+	513:516	arg1	cations					527:533	Ca2+ and Mg2+ cations	513:533	Ca2+ and Mg2+ cations	513:533	The slow rates of scaffold biodegradation were achieved by using Ca2+ and Mg2+ cations as cross-linking agents.					
28972200	3	7	theme	Ca2+	513:516	arg1	agents					552:557	cross-linking agents	538:557	cross-linking agents	538:557	The slow rates of scaffold biodegradation were achieved by using Ca2+ and Mg2+ cations as cross-linking agents.					
28972200	1	8	theme	grafting	178:185	arg1	therapy					187:193	grafting therapy	178:193	grafting therapy	178:193	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	4	9	theme	electron	678:685	arg1	microscopy					687:696	scanning electron microscopy	669:696	scanning electron microscopy	669:696	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	5	10	theme	AG/FG	879:883	arg1	ratio					885:889	AG/FG ratio	879:889	AG/FG ratio	879:889	The scaffolds were characterized by high porosity and tensile strength, possessing average pore size from about 60 to 300 μm depending on AG/FG ratio and fibrin stabilization.					
28972200	7	11	theme	higher	1381:1386	arg1	content					1391:1397	higher FG content	1381:1397	higher FG content	1381:1397	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	4	12	theme	porous	631:636	arg1	structure					638:646	highly interconnected porous structure	609:646	highly interconnected porous structure	609:646	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	10	13	theme	grafting	1757:1764	arg1	material					1766:1773	a grafting material	1755:1773	a grafting material by itself	1755:1783	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	10	13	theme	grafting	1757:1764	arg1	sponges					1719:1725	thrombin-modified alginate sponges	1692:1725	thrombin-modified alginate sponges	1692:1725	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	10	14	used	used	1747:1750	arg2	sponges					1719:1725	thrombin-modified alginate sponges	1692:1725	thrombin-modified alginate sponges	1692:1725	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	10	14	used	used	1747:1750	arg2	material					1766:1773	a grafting material	1755:1773	a grafting material by itself	1755:1783	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	6	15	theme	different	976:984	arg1	ratio					992:996	a different AG/FG ratio	974:996	a different AG/FG ratio	974:996	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	10	16	theme	skin	1796:1799	arg1	healing					1801:1807	skin healing	1796:1807	skin healing	1796:1807	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	6	17	with	cells	1166:1170	arg1	applicability					1039:1051	potential applicability	1029:1051	potential applicability to skin tissue engineering	1029:1078	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	4	18	theme	pycnometric	719:729	arg1	analysis					731:738	pycnometric analysis	719:738	pycnometric analysis	719:738	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	1	19	theme	pro-angiogenic	108:121	arg1	material					132:139	a new pro-angiogenic scaffold material	102:139	a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy	102:193	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	6	20	theme	thrombin-modified	941:957	arg1	scaffolds					959:967	thrombin-modified scaffolds	941:967	thrombin-modified scaffolds	941:967	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	7	21	theme	cells	1340:1344	arg1	types					1331:1335	all types	1327:1335	all types of cells	1327:1344	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	5	22	theme	high	777:780	arg1	porosity					782:789	high porosity	777:789	high porosity	777:789	The scaffolds were characterized by high porosity and tensile strength, possessing average pore size from about 60 to 300 μm depending on AG/FG ratio and fibrin stabilization.					
28972200	1	23	theme	freeze-drying	301:313	arg1	approach					315:322	the freeze-drying approach	297:322	the freeze-drying approach	297:322	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	5	24	from	μm	863:864	arg1	size					837:840	average pore size	824:840	average pore size from about 60 to 300 μm	824:864	The scaffolds were characterized by high porosity and tensile strength, possessing average pore size from about 60 to 300 μm depending on AG/FG ratio and fibrin stabilization.					
28972200	8	25	theme	aortic	1468:1473	arg1	sprouting					1480:1488	the aortic ring sprouting	1464:1488	the aortic ring sprouting	1464:1488	In unstabilized scaffolds, the addition of FG did not stimulate the aortic ring sprouting.					
28972200	7	26	theme	low	1247:1249	arg1	content					1260:1266	low (15%) FG content	1247:1266	low (15%) FG content	1247:1266	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	6	27	theme	human	1012:1016	arg1	cells					1018:1022	human cells	1012:1022	human cells with potential applicability to skin tissue engineering	1012:1078	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	6	28	theme	adipose-derived	1197:1211	arg1	cells					1221:1225	subcutaneous adipose-derived stromal cells	1184:1225	subcutaneous adipose-derived stromal cells	1184:1225	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	9	29	theme	fibrin	1509:1514	arg1	stabilization					1516:1528	fibrin stabilization	1509:1528	fibrin stabilization by εAc	1509:1535	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	0	30	theme	scaffold	41:48	arg1	material					50:57	a scaffold material	39:57	a scaffold material for skin tissue engineering	39:85	Fibrinogen-modified sodium alginate as a scaffold material for skin tissue engineering.					
28972200	9	31	theme	same	1498:1501	arg1	time					1503:1506	the same time	1494:1506	the same time	1494:1506	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	0	32	theme	Fibrinogen-modified	0:18	arg1	alginate					27:34	Fibrinogen-modified sodium alginate	0:34	Fibrinogen-modified sodium alginate	0:34	Fibrinogen-modified sodium alginate as a scaffold material for skin tissue engineering.					
28972200	1	33	theme	-fibrinogen	257:267	arg1	scaffolds					281:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	6	34	theme	immortalized	1081:1092	arg1	N-TERT					1119:1124	N-TERT	1119:1124	N-TERT	1119:1124	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	6	34	theme	immortalized	1081:1092	arg1	keratinocytes					1104:1116	immortalized epidermal keratinocytes	1081:1116	immortalized epidermal keratinocytes (N-TERT)	1081:1125	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	6	35	with	cells	1018:1022	arg1	applicability					1039:1051	potential applicability	1029:1051	potential applicability to skin tissue engineering	1029:1078	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	10	36	theme	obtained	1662:1669	arg1	results					1671:1677	obtained results	1662:1677	obtained results	1662:1677	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	1	37	theme	FG	270:271	arg1	scaffolds					281:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	9	38	theme	significant	1549:1559	arg1	increase					1561:1568	significant increase	1549:1568	significant increase of aortic ring sprouting	1549:1593	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	1	39	theme	sponge	274:279	arg1	scaffolds					281:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	6	40	theme	skin	1056:1059	arg1	engineering					1068:1078	skin tissue engineering	1056:1078	skin tissue engineering	1056:1078	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	0	41	theme	skin	63:66	arg1	engineering					75:85	skin tissue engineering	63:85	skin tissue engineering	63:85	Fibrinogen-modified sodium alginate as a scaffold material for skin tissue engineering.					
28972200	10	42	theme	thrombin-modified	1692:1708	arg1	sponges					1719:1725	thrombin-modified alginate sponges	1692:1725	thrombin-modified alginate sponges	1692:1725	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	10	42	theme	thrombin-modified	1692:1708	arg1	material					1766:1773	a grafting material	1755:1773	a grafting material by itself	1755:1783	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	9	43	theme	ring	1580:1583	arg1	sprouting					1585:1593	aortic ring sprouting	1573:1593	aortic ring sprouting	1573:1593	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	7	44	theme	highest	1283:1289	arg1	adhesiveness					1291:1302	adhesiveness	1291:1302	adhesiveness	1291:1302	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	8	45	theme	unstabilized	1403:1414	arg1	scaffolds					1416:1424	unstabilized scaffolds	1403:1424	unstabilized scaffolds	1403:1424	In unstabilized scaffolds, the addition of FG did not stimulate the aortic ring sprouting.					
28972200	3	46	theme	slow	452:455	arg1	rates					457:461	The slow rates	448:461	The slow rates of scaffold biodegradation	448:488	The slow rates of scaffold biodegradation were achieved by using Ca2+ and Mg2+ cations as cross-linking agents.					
28972200	0	47	dep	alginate	27:34	arg1	material					50:57	a scaffold material	39:57	a scaffold material for skin tissue engineering	39:85	Fibrinogen-modified sodium alginate as a scaffold material for skin tissue engineering.					
28972200	6	48	with	biocompatibility	921:936	arg1	ratio					992:996	a different AG/FG ratio	974:996	a different AG/FG ratio	974:996	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	4	49	theme	AG-FG	588:592	arg1	scaffolds					594:602	The novel thrombin-modified AG-FG scaffolds	560:602	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure	560:646	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	10	50	theme	three-dimensional	1853:1869	arg1	construction					1885:1896	three-dimensional bioequivalent construction	1853:1896	three-dimensional bioequivalent construction	1853:1896	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	1	51	theme	composite	227:235	arg1	scaffolds					281:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	6	52	with	fibroblasts	1141:1151	arg1	applicability					1039:1051	potential applicability	1029:1051	potential applicability to skin tissue engineering	1029:1078	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	9	53	theme	efficient	1604:1612	arg1	formation					1614:1622	more efficient formation	1599:1622	more efficient formation of microvascular network	1599:1647	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	3	54	theme	biodegradation	475:488	arg1	rates					457:461	The slow rates	448:461	The slow rates of scaffold biodegradation	448:488	The slow rates of scaffold biodegradation were achieved by using Ca2+ and Mg2+ cations as cross-linking agents.					
28972200	1	55	theme	alginate	244:251	arg1	scaffolds					281:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	1	56	theme	AG	254:255	arg1	scaffolds					281:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	9	57	theme	network	1641:1647	arg1	increase					1561:1568	significant increase	1549:1568	significant increase of aortic ring sprouting	1549:1593	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	9	57	theme	network	1641:1647	arg1	formation					1614:1622	more efficient formation	1599:1622	more efficient formation of microvascular network	1599:1647	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	5	58	theme	fibrin	895:900	arg1	stabilization					902:914	fibrin stabilization	895:914	fibrin stabilization	895:914	The scaffolds were characterized by high porosity and tensile strength, possessing average pore size from about 60 to 300 μm depending on AG/FG ratio and fibrin stabilization.					
28972200	4	59	theme	novel	564:568	arg1	scaffolds					594:602	The novel thrombin-modified AG-FG scaffolds	560:602	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure	560:646	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	3	60	theme	cross-linking	538:550	arg1	cations					527:533	Ca2+ and Mg2+ cations	513:533	Ca2+ and Mg2+ cations	513:533	The slow rates of scaffold biodegradation were achieved by using Ca2+ and Mg2+ cations as cross-linking agents.					
28972200	3	60	theme	cross-linking	538:550	arg1	agents					552:557	cross-linking agents	538:557	cross-linking agents	538:557	The slow rates of scaffold biodegradation were achieved by using Ca2+ and Mg2+ cations as cross-linking agents.					
28972200	4	61	theme	scanning	669:676	arg1	microscopy					687:696	scanning electron microscopy	669:696	scanning electron microscopy	669:696	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	6	62	theme	AG/FG	986:990	arg1	ratio					992:996	a different AG/FG ratio	974:996	a different AG/FG ratio	974:996	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	7	63	theme	FG	1388:1389	arg1	content					1391:1397	higher FG content	1381:1397	higher FG content	1381:1397	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	4	64	theme	interconnected	616:629	arg1	structure					638:646	highly interconnected porous structure	609:646	highly interconnected porous structure	609:646	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	6	65	with	cells	1221:1225	arg1	applicability					1039:1051	potential applicability	1029:1051	potential applicability to skin tissue engineering	1029:1078	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	8	66	theme	FG	1443:1444	arg1	addition					1431:1438	the addition	1427:1438	the addition of FG	1427:1444	In unstabilized scaffolds, the addition of FG did not stimulate the aortic ring sprouting.					
28972200	4	67	with	scaffolds	594:602	arg1	structure					638:646	highly interconnected porous structure	609:646	highly interconnected porous structure	609:646	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	1	68	theme	new	104:106	arg1	material					132:139	a new pro-angiogenic scaffold material	102:139	a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy	102:193	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	6	69	theme	scaffolds	959:967	arg1	biocompatibility					921:936	The biocompatibility	917:936	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio	917:996	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	7	70	with	scaffolds	1232:1240	arg1	content					1260:1266	low (15%) FG content	1247:1266	low (15%) FG content	1247:1266	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	9	71	theme	sprouting	1585:1593	arg1	increase					1561:1568	significant increase	1549:1568	significant increase of aortic ring sprouting	1549:1593	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	9	71	theme	sprouting	1585:1593	arg1	formation					1614:1622	more efficient formation	1599:1622	more efficient formation of microvascular network	1599:1647	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	1	72	theme	scaffold	123:130	arg1	material					132:139	a new pro-angiogenic scaffold material	102:139	a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy	102:193	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	0	73	theme	tissue	68:73	arg1	engineering					75:85	skin tissue engineering	63:85	skin tissue engineering	63:85	Fibrinogen-modified sodium alginate as a scaffold material for skin tissue engineering.					
28972200	1	74	theme	suitable	141:148	arg1	material					132:139	a new pro-angiogenic scaffold material	102:139	a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy	102:193	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	6	75	theme	potential	1029:1037	arg1	applicability					1039:1051	potential applicability	1029:1051	potential applicability to skin tissue engineering	1029:1078	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	8	76	theme	ring	1475:1478	arg1	sprouting					1480:1488	the aortic ring sprouting	1464:1488	the aortic ring sprouting	1464:1488	In unstabilized scaffolds, the addition of FG did not stimulate the aortic ring sprouting.					
28972200	6	77	link	adipose-derived	1197:1211	arg1	cells					1221:1225	subcutaneous adipose-derived stromal cells	1184:1225	subcutaneous adipose-derived stromal cells	1184:1225	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	1	78	theme	skin	154:157	arg1	bioengineering					159:172	skin bioengineering	154:172	skin bioengineering	154:172	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	4	79	theme	tensile	699:705	arg1	testing					707:713	tensile testing	699:713	tensile testing	699:713	The novel thrombin-modified AG-FG scaffolds with highly interconnected porous structure were evaluated using scanning electron microscopy, tensile testing and pycnometric analysis.					
28972200	6	80	theme	skin	1136:1139	arg1	fibroblasts					1141:1151	primary skin fibroblasts	1128:1151	primary skin fibroblasts	1128:1151	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	7	81	with	scaffolds	1366:1374	arg1	content					1391:1397	higher FG content	1381:1397	higher FG content	1381:1397	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	0	82	theme	sodium	20:25	arg1	alginate					27:34	Fibrinogen-modified sodium alginate	0:34	Fibrinogen-modified sodium alginate	0:34	Fibrinogen-modified sodium alginate as a scaffold material for skin tissue engineering.					
28972200	6	83	theme	epidermal	1094:1102	arg1	N-TERT					1119:1124	N-TERT	1119:1124	N-TERT	1119:1124	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	6	83	theme	epidermal	1094:1102	arg1	keratinocytes					1104:1116	immortalized epidermal keratinocytes	1081:1116	immortalized epidermal keratinocytes (N-TERT)	1081:1125	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	2	84	theme	FG/fibrin	353:361	arg1	conversion					363:372	FG/fibrin conversion	353:372	FG/fibrin conversion	353:372	Thrombin was added to drive FG/fibrin conversion, while ε-aminocapronic acid (εAc) was used as antifibrinolytic component.					
28972200	5	85	theme	pore	832:835	arg1	size					837:840	average pore size	824:840	average pore size from about 60 to 300 μm	824:864	The scaffolds were characterized by high porosity and tensile strength, possessing average pore size from about 60 to 300 μm depending on AG/FG ratio and fibrin stabilization.					
28972200	7	86	theme	survival	1308:1315	arg1	rates					1317:1321	survival rates	1308:1321	survival rates	1308:1321	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	7	87	dep	low	1247:1249	arg1	%					1254:1254	15%	1252:1254	15%	1252:1254	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	6	88	theme	primary	1128:1134	arg1	fibroblasts					1141:1151	primary skin fibroblasts	1128:1151	primary skin fibroblasts	1128:1151	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	5	89	theme	tensile	795:801	arg1	strength					803:810	tensile strength	795:810	tensile strength	795:810	The scaffolds were characterized by high porosity and tensile strength, possessing average pore size from about 60 to 300 μm depending on AG/FG ratio and fibrin stabilization.					
28972200	6	90	theme	tissue	1061:1066	arg1	engineering					1068:1078	skin tissue engineering	1056:1078	skin tissue engineering	1056:1078	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	2	91	theme	ε-aminocapronic	381:395	arg1	acid					397:400	ε-aminocapronic acid	381:400	ε-aminocapronic acid (εAc)	381:406	Thrombin was added to drive FG/fibrin conversion, while ε-aminocapronic acid (εAc) was used as antifibrinolytic component.					
28972200	2	91	theme	ε-aminocapronic	381:395	arg1	εAc					403:405	εAc	403:405	εAc	403:405	Thrombin was added to drive FG/fibrin conversion, while ε-aminocapronic acid (εAc) was used as antifibrinolytic component.					
28972200	2	91	theme	ε-aminocapronic	381:395	arg1	component					437:445	antifibrinolytic component	420:445	antifibrinolytic component	420:445	Thrombin was added to drive FG/fibrin conversion, while ε-aminocapronic acid (εAc) was used as antifibrinolytic component.					
28972200	1	92	theme	scaffolds	281:289	arg1	number					217:222	a number	215:222	a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	215:289	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
28972200	6	93	with	keratinocytes	1104:1116	arg1	applicability					1039:1051	potential applicability	1029:1051	potential applicability to skin tissue engineering	1029:1078	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	9	94	theme	aortic	1573:1578	arg1	sprouting					1585:1593	aortic ring sprouting	1573:1593	aortic ring sprouting	1573:1593	At the same time, fibrin stabilization by εAc resulted in significant increase of aortic ring sprouting and more efficient formation of microvascular network.					
28972200	6	95	theme	endothelial	1154:1164	arg1	HUVEC					1173:1177	HUVEC	1173:1177	HUVEC	1173:1177	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	6	95	theme	endothelial	1154:1164	arg1	cells					1166:1170	endothelial cells	1154:1170	endothelial cells (HUVEC)	1154:1178	The biocompatibility of thrombin-modified scaffolds with a different AG/FG ratio was tested on human cells with potential applicability to skin tissue engineering: immortalized epidermal keratinocytes (N-TERT), primary skin fibroblasts, endothelial cells (HUVEC) and subcutaneous adipose-derived stromal cells.					
28972200	2	96	theme	antifibrinolytic	420:435	arg1	component					437:445	antifibrinolytic component	420:445	antifibrinolytic component	420:445	Thrombin was added to drive FG/fibrin conversion, while ε-aminocapronic acid (εAc) was used as antifibrinolytic component.					
28972200	2	96	theme	antifibrinolytic	420:435	arg1	acid					397:400	ε-aminocapronic acid	381:400	ε-aminocapronic acid (εAc)	381:406	Thrombin was added to drive FG/fibrin conversion, while ε-aminocapronic acid (εAc) was used as antifibrinolytic component.					
28972200	3	97	theme	scaffold	466:473	arg1	biodegradation					475:488	scaffold biodegradation	466:488	scaffold biodegradation	466:488	The slow rates of scaffold biodegradation were achieved by using Ca2+ and Mg2+ cations as cross-linking agents.					
28972200	7	98	dep	adhesiveness	1291:1302	arg1	the					1279:1281	the	1279:1281	the	1279:1281	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	10	99	theme	bioequivalent	1871:1883	arg1	construction					1885:1896	three-dimensional bioequivalent construction	1853:1896	three-dimensional bioequivalent construction	1853:1896	Altogether, obtained results suggest that thrombin-modified alginate sponges can be successfully used as a grafting material by itself to promote skin healing and regeneration and also as a scaffold for three-dimensional bioequivalent construction.					
28972200	5	100	dep	300	859:861	arg1	to					856:857	to	856:857	to	856:857	The scaffolds were characterized by high porosity and tensile strength, possessing average pore size from about 60 to 300 μm depending on AG/FG ratio and fibrin stabilization.					
28972200	7	101	theme	FG	1257:1258	arg1	content					1260:1266	low (15%) FG content	1247:1266	low (15%) FG content	1247:1266	The scaffolds with low (15%) FG content have shown the highest adhesiveness and survival rates for all types of cells, as compared to the scaffolds with higher FG content.					
28972200	5	102	theme	average	824:830	arg1	size					837:840	average pore size	824:840	average pore size from about 60 to 300 μm	824:864	The scaffolds were characterized by high porosity and tensile strength, possessing average pore size from about 60 to 300 μm depending on AG/FG ratio and fibrin stabilization.					
28972200	1	103	theme	sodium	237:242	arg1	scaffolds					281:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds	227:289	In search for a new pro-angiogenic scaffold material suitable for skin bioengineering and grafting therapy, we have fabricated a number of composite sodium alginate (AG)-fibrinogen (FG) sponge scaffolds using the freeze-drying approach.					
31260238	2	0	theme	strong	429:434	arg1	interaction					450:460	strong electrostatic interaction	429:460	strong electrostatic interaction	429:460	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	1	1	theme	oxide	308:312	arg1	coating					321:327	nanoscale chitosan/graphene oxide hybrid coating	280:327	nanoscale chitosan/graphene oxide hybrid coating	280:327	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	0	2	theme	Therapeutic	90:100	arg1	Applications					102:113	Potential Multimodal Therapeutic Applications	69:113	Potential Multimodal Therapeutic Applications	69:113	Nanoscale Hybrid Coating Enables Multifunctional Tissue Scaffold for Potential Multimodal Therapeutic Applications.					
31260238	1	3	theme	unique	190:195	arg1	scaffold					220:227	a unique multifunctional tissue scaffold	188:227	a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating	188:327	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	1	4	theme	hybrid	314:319	arg1	coating					321:327	nanoscale chitosan/graphene oxide hybrid coating	280:327	nanoscale chitosan/graphene oxide hybrid coating	280:327	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	2	5	with	composition	573:583	arg1	control					516:522	control	516:522	control over structural element orientation	516:558	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	2	6	theme	drug-laden	352:361	arg1	chitosan					363:370	chitosan	363:370	chitosan	363:370	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	2	7	theme	chitosan	363:370	arg1	layers					342:347	Alternative layers	330:347	Alternative layers of drug-laden chitosan and graphene oxide nanosheets	330:400	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	4	8	theme	bone	889:892	arg1	managements					901:911	bone cancer managements	889:911	bone cancer managements	889:911	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	3	9	theme	Combined	599:606	arg1	co-delivery					622:632	Combined pH-controlled co-delivery	599:632	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy	599:688	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy has been achieved by our scaffold system.					
31260238	4	10	theme	potential	835:843	arg1	managements					901:911	bone cancer managements	889:911	bone cancer managements	889:911	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	4	10	theme	potential	835:843	arg1	applications					868:879	potential multimodal therapeutic applications	835:879	potential multimodal therapeutic applications such as bone cancer managements	835:911	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	4	11	theme	platform	740:747	arg1	technology					749:758	The new platform technology	732:758	The new platform technology	732:758	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	3	12	theme	multiple	637:644	arg1	agents					658:663	multiple therapeutic agents	637:663	multiple therapeutic agents	637:663	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy has been achieved by our scaffold system.					
31260238	1	13	theme	multifunctional	197:211	arg1	scaffold					220:227	a unique multifunctional tissue scaffold	188:227	a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating	188:327	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	2	14	with	architecture	498:509	arg1	control					516:522	control	516:522	control over structural element orientation	516:558	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	2	15	theme	chemical	564:571	arg1	composition					573:583	chemical composition	564:583	chemical composition	564:583	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	2	16	theme	Alternative	330:340	arg1	layers					342:347	Alternative layers	330:347	Alternative layers of drug-laden chitosan and graphene oxide nanosheets	330:400	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	1	17	theme	tissue	213:218	arg1	scaffold					220:227	a unique multifunctional tissue scaffold	188:227	a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating	188:327	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	0	18	theme	Hybrid	10:15	arg1	Coating					17:23	Nanoscale Hybrid Coating	0:23	Nanoscale Hybrid Coating	0:23	Nanoscale Hybrid Coating Enables Multifunctional Tissue Scaffold for Potential Multimodal Therapeutic Applications.					
31260238	2	19	theme	oxide	385:389	arg1	nanosheets					391:400	graphene oxide nanosheets	376:400	graphene oxide nanosheets	376:400	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	4	20	theme	other	790:794	arg1	systems					812:818	other tissue scaffold systems	790:818	other tissue scaffold systems	790:818	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	0	21	theme	Nanoscale	0:8	arg1	Coating					17:23	Nanoscale Hybrid Coating	0:23	Nanoscale Hybrid Coating	0:23	Nanoscale Hybrid Coating Enables Multifunctional Tissue Scaffold for Potential Multimodal Therapeutic Applications.					
31260238	3	22	theme	therapy	682:688	arg1	co-delivery					622:632	Combined pH-controlled co-delivery	599:632	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy	599:688	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy has been achieved by our scaffold system.					
31260238	2	23	theme	graphene	376:383	arg1	nanosheets					391:400	graphene oxide nanosheets	376:400	graphene oxide nanosheets	376:400	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	4	24	theme	cancer	894:899	arg1	managements					901:911	bone cancer managements	889:911	bone cancer managements	889:911	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	2	25	theme	electrostatic	436:448	arg1	interaction					450:460	strong electrostatic interaction	429:460	strong electrostatic interaction	429:460	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	3	26	theme	photothermal	669:680	arg1	therapy					682:688	photothermal therapy	669:688	photothermal therapy	669:688	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy has been achieved by our scaffold system.					
31260238	4	27	theme	therapeutic	856:866	arg1	managements					901:911	bone cancer managements	889:911	bone cancer managements	889:911	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	4	27	theme	therapeutic	856:866	arg1	applications					868:879	potential multimodal therapeutic applications	835:879	potential multimodal therapeutic applications such as bone cancer managements	835:911	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	1	28	theme	nature-inspired	126:140	arg1	process					166:172	a nature-inspired layer-by-layer assembly process	124:172	a nature-inspired layer-by-layer assembly process	124:172	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	2	29	theme	multilayer	487:496	arg1	architecture					498:509	a robust multilayer architecture	478:509	a robust multilayer architecture with control over structural element orientation	478:558	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	0	30	theme	Tissue	49:54	arg1	Scaffold					56:63	Multifunctional Tissue Scaffold	33:63	Multifunctional Tissue Scaffold for Potential Multimodal Therapeutic Applications	33:113	Nanoscale Hybrid Coating Enables Multifunctional Tissue Scaffold for Potential Multimodal Therapeutic Applications.					
31260238	2	31	theme	robust	480:485	arg1	architecture					498:509	a robust multilayer architecture	478:509	a robust multilayer architecture with control over structural element orientation	478:558	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	1	32	theme	porous	246:251	arg1	substrate					266:274	porous polyurethane substrate	246:274	porous polyurethane substrate	246:274	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	0	33	theme	Multifunctional	33:47	arg1	Scaffold					56:63	Multifunctional Tissue Scaffold	33:63	Multifunctional Tissue Scaffold for Potential Multimodal Therapeutic Applications	33:113	Nanoscale Hybrid Coating Enables Multifunctional Tissue Scaffold for Potential Multimodal Therapeutic Applications.					
31260238	3	34	theme	scaffold	715:722	arg1	system					724:729	our scaffold system	711:729	our scaffold system	711:729	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy has been achieved by our scaffold system.					
31260238	1	35	theme	polyurethane	253:264	arg1	substrate					266:274	porous polyurethane substrate	246:274	porous polyurethane substrate	246:274	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	1	36	theme	layer-by-layer	142:155	arg1	process					166:172	a nature-inspired layer-by-layer assembly process	124:172	a nature-inspired layer-by-layer assembly process	124:172	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	2	37	theme	element	540:546	arg1	orientation					548:558	structural element orientation	529:558	structural element orientation	529:558	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	3	38	theme	pH-controlled	608:620	arg1	co-delivery					622:632	Combined pH-controlled co-delivery	599:632	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy	599:688	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy has been achieved by our scaffold system.					
31260238	1	39	theme	assembly	157:164	arg1	process					166:172	a nature-inspired layer-by-layer assembly process	124:172	a nature-inspired layer-by-layer assembly process	124:172	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	4	40	theme	tissue	796:801	arg1	systems					812:818	other tissue scaffold systems	790:818	other tissue scaffold systems	790:818	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	4	41	theme	multimodal	845:854	arg1	managements					901:911	bone cancer managements	889:911	bone cancer managements	889:911	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	4	41	theme	multimodal	845:854	arg1	applications					868:879	potential multimodal therapeutic applications	835:879	potential multimodal therapeutic applications such as bone cancer managements	835:911	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	3	42	theme	therapeutic	646:656	arg1	agents					658:663	multiple therapeutic agents	637:663	multiple therapeutic agents	637:663	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy has been achieved by our scaffold system.					
31260238	3	43	theme	agents	658:663	arg1	co-delivery					622:632	Combined pH-controlled co-delivery	599:632	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy	599:688	Combined pH-controlled co-delivery of multiple therapeutic agents and photothermal therapy has been achieved by our scaffold system.					
31260238	2	44	theme	nanosheets	391:400	arg1	layers					342:347	Alternative layers	330:347	Alternative layers of drug-laden chitosan and graphene oxide nanosheets	330:400	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	0	45	theme	Multimodal	79:88	arg1	Applications					102:113	Potential Multimodal Therapeutic Applications	69:113	Potential Multimodal Therapeutic Applications	69:113	Nanoscale Hybrid Coating Enables Multifunctional Tissue Scaffold for Potential Multimodal Therapeutic Applications.					
31260238	4	46	theme	new	736:738	arg1	technology					749:758	The new platform technology	732:758	The new platform technology	732:758	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	4	47	theme	scaffold	803:810	arg1	systems					812:818	other tissue scaffold systems	790:818	other tissue scaffold systems	790:818	The new platform technology can be generalized to produce other tissue scaffold systems and may enable potential multimodal therapeutic applications such as bone cancer managements.					
31260238	1	48	theme	nanoscale	280:288	arg1	coating					321:327	nanoscale chitosan/graphene oxide hybrid coating	280:327	nanoscale chitosan/graphene oxide hybrid coating	280:327	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
31260238	0	49	theme	Potential	69:77	arg1	Applications					102:113	Potential Multimodal Therapeutic Applications	69:113	Potential Multimodal Therapeutic Applications	69:113	Nanoscale Hybrid Coating Enables Multifunctional Tissue Scaffold for Potential Multimodal Therapeutic Applications.					
31260238	2	50	theme	structural	529:538	arg1	element					540:546	structural element	529:546	structural element orientation	529:558	Alternative layers of drug-laden chitosan and graphene oxide nanosheets were held together through strong electrostatic interaction, giving rise to a robust multilayer architecture with control over structural element orientation and chemical composition at nanoscale.					
31260238	1	51	theme	chitosan/graphene	290:306	arg1	coating					321:327	nanoscale chitosan/graphene oxide hybrid coating	280:327	nanoscale chitosan/graphene oxide hybrid coating	280:327	Through a nature-inspired layer-by-layer assembly process, we developed a unique multifunctional tissue scaffold that consists of porous polyurethane substrate and nanoscale chitosan/graphene oxide hybrid coating.					
30953739	0	0	theme	spinal	81:86	arg1	injury					93:98	spinal cord injury	81:98	spinal cord injury	81:98	Upregulation of proteoglycans in the perilesion perimeter in ventral horns after spinal cord injury.					
30953739	3	1	theme	perilesion	457:466	arg1	area					451:454	the damaged area	439:454	the damaged area (perilesion perimeter)	439:477	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	3	1	theme	perilesion	457:466	arg1	perimeter					468:476	perilesion perimeter	457:476	perilesion perimeter	457:476	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	1	2	theme	axon	222:225	arg1	regeneration					227:238	axon regeneration	222:238	axon regeneration	222:238	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	6	3	theme	neuronal	1012:1019	arg1	dysfunction					1021:1031	neuronal dysfunction	1012:1031	neuronal dysfunction far from the damaged area	1012:1057	The results of the study suggest that SCI induces widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI.					
30953739	1	4	theme	pronounced	137:146	arg1	damage					161:166	pronounced focal tissue damage	137:166	pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth	137:251	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	0	5	from	Upregulation	0:11	arg1	perimeter					48:56	the perilesion perimeter	33:56	the perilesion perimeter in ventral horns after spinal cord injury	33:98	Upregulation of proteoglycans in the perilesion perimeter in ventral horns after spinal cord injury.					
30953739	2	6	theme	distal	330:335	arg1	regions					322:328	regions	322:328	regions distal from the injured area	322:357	Cellular changes and the composition of the extracellular matrix in regions distal from the injured area remain poorly characterized.					
30953739	3	7	theme	gross	498:502	arg1	changes					517:523	minimal gross histological changes	490:523	minimal gross histological changes	490:523	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	0	8	theme	cord	88:91	arg1	injury					93:98	spinal cord injury	81:98	spinal cord injury	81:98	Upregulation of proteoglycans in the perilesion perimeter in ventral horns after spinal cord injury.					
30953739	4	9	theme	astrocytic	723:732	arg1	transporter					744:754	main astrocytic glutamate transporter 1	718:756	main astrocytic glutamate transporter 1	718:756	These abnormalities coincided with the appearance of reactive astrocytes and a reduction in main astrocytic glutamate transporter 1.					
30953739	3	10	theme	damaged	443:449	arg1	area					451:454	the damaged area	439:454	the damaged area (perilesion perimeter)	439:477	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	3	10	theme	damaged	443:449	arg1	perimeter					468:476	perilesion perimeter	457:476	perilesion perimeter	457:476	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	0	11	from	perimeter	48:56	arg1	horns					69:73	ventral horns	61:73	ventral horns	61:73	Upregulation of proteoglycans in the perilesion perimeter in ventral horns after spinal cord injury.					
30953739	6	12	from	changes	957:963	arg1	cord					979:982	the spinal cord	968:982	the spinal cord	968:982	The results of the study suggest that SCI induces widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI.					
30953739	2	13	theme	Cellular	254:261	arg1	changes					263:269	Cellular changes	254:269	Cellular changes	254:269	Cellular changes and the composition of the extracellular matrix in regions distal from the injured area remain poorly characterized.					
30953739	1	14	theme	focal	148:152	arg1	damage					161:166	pronounced focal tissue damage	137:166	pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth	137:251	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	6	15	theme	widespread	946:955	arg1	changes					957:963	widespread changes	946:963	widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI	946:1092	The results of the study suggest that SCI induces widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI.					
30953739	3	16	theme	minimal	490:496	arg1	changes					517:523	minimal gross histological changes	490:523	minimal gross histological changes	490:523	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	6	17	theme	damaged	1046:1052	arg1	area					1054:1057	the damaged area	1042:1057	the damaged area	1042:1057	The results of the study suggest that SCI induces widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI.					
30953739	1	18	theme	tissue	154:159	arg1	damage					161:166	pronounced focal tissue damage	137:166	pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth	137:251	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	4	19	from	appearance	665:674	arg1	transporter					744:754	main astrocytic glutamate transporter 1	718:756	main astrocytic glutamate transporter 1	718:756	These abnormalities coincided with the appearance of reactive astrocytes and a reduction in main astrocytic glutamate transporter 1.					
30953739	5	20	from	kinetics	799:806	arg1	areas					872:876	areas	872:876	areas distal from them	872:893	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	5	20	from	kinetics	799:806	arg1	them					890:893	them	890:893	them	890:893	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	5	20	from	kinetics	799:806	arg1	areas					833:837	areas	833:837	areas near neuronal cell bodies	833:863	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	5	21	from	changes	812:818	arg1	areas					872:876	areas	872:876	areas distal from them	872:893	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	5	21	from	changes	812:818	arg1	them					890:893	them	890:893	them	890:893	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	5	21	from	changes	812:818	arg1	areas					833:837	areas	833:837	areas near neuronal cell bodies	833:863	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	5	22	theme	cell	853:856	arg1	bodies					858:863	neuronal cell bodies	844:863	neuronal cell bodies	844:863	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	4	23	theme	astrocytes	688:697	arg1	appearance					665:674	the appearance	661:674	the appearance of reactive astrocytes	661:697	These abnormalities coincided with the appearance of reactive astrocytes and a reduction in main astrocytic glutamate transporter 1.					
30953739	4	23	theme	astrocytes	688:697	arg1	reduction					705:713	a reduction	703:713	a reduction in main astrocytic glutamate transporter 1	703:756	These abnormalities coincided with the appearance of reactive astrocytes and a reduction in main astrocytic glutamate transporter 1.					
30953739	4	24	from	reduction	705:713	arg1	transporter					744:754	main astrocytic glutamate transporter 1	718:756	main astrocytic glutamate transporter 1	718:756	These abnormalities coincided with the appearance of reactive astrocytes and a reduction in main astrocytic glutamate transporter 1.					
30953739	5	25	theme	neuronal	844:851	arg1	bodies					858:863	neuronal cell bodies	844:863	neuronal cell bodies	844:863	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	5	26	from	them	890:893	arg1	areas					872:876	areas	872:876	areas distal from them	872:893	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	5	26	from	them	890:893	arg1	kinetics					799:806	different kinetics	789:806	different kinetics	789:806	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	5	26	from	them	890:893	arg1	changes					812:818	changes	812:818	changes after SCI in areas near neuronal cell bodies and in areas distal from them	812:893	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	4	27	theme	main	718:721	arg1	transporter					744:754	main astrocytic glutamate transporter 1	718:756	main astrocytic glutamate transporter 1	718:756	These abnormalities coincided with the appearance of reactive astrocytes and a reduction in main astrocytic glutamate transporter 1.					
30953739	1	28	theme	subsequent	173:182	arg1	formation					184:192	subsequent formation	173:192	subsequent formation of a glial scar that blocks axon regeneration and regrowth	173:251	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	0	29	theme	proteoglycans	16:28	arg1	Upregulation					0:11	Upregulation	0:11	Upregulation of proteoglycans in the perilesion perimeter in ventral horns after spinal cord injury.	0:99	Upregulation of proteoglycans in the perilesion perimeter in ventral horns after spinal cord injury.					
30953739	3	30	dep	gross	498:502	arg1	histological					504:515	histological	504:515	histological	504:515	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	1	31	theme	Spinal	101:106	arg1	SCI					121:123	SCI	121:123	SCI	121:123	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	1	31	theme	Spinal	101:106	arg1	injury					113:118	Spinal cord injury	101:118	Spinal cord injury (SCI)	101:124	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	3	32	from	alterations	552:562	arg1	proteoglycans					585:597	the extracellular proteoglycans	567:597	the extracellular proteoglycans	567:597	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	6	33	theme	further	1063:1069	arg1	aggravation					1071:1081	further aggravation	1063:1081	further aggravation of the SCI	1063:1092	The results of the study suggest that SCI induces widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI.					
30953739	0	34	theme	perilesion	37:46	arg1	perimeter					48:56	the perilesion perimeter	33:56	the perilesion perimeter in ventral horns after spinal cord injury	33:98	Upregulation of proteoglycans in the perilesion perimeter in ventral horns after spinal cord injury.					
30953739	3	35	theme	spinal	417:422	arg1	cord					424:427	the spinal cord	413:427	the spinal cord distal to the damaged area (perilesion perimeter)	413:477	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	3	36	theme	present	395:401	arg1	study					403:407	the present study	391:407	the present study	391:407	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	6	37	theme	study	915:919	arg1	results					900:906	The results	896:906	The results of the study	896:919	The results of the study suggest that SCI induces widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI.					
30953739	5	38	theme	Proteoglycan	759:770	arg1	levels					772:777	Proteoglycan levels	759:777	Proteoglycan levels	759:777	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	1	39	with	damage	161:166	arg1	formation					184:192	subsequent formation	173:192	subsequent formation of a glial scar that blocks axon regeneration and regrowth	173:251	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	2	40	from	area	354:357	arg1	distal					330:335	distal	330:335	distal	330:335	Cellular changes and the composition of the extracellular matrix in regions distal from the injured area remain poorly characterized.					
30953739	1	41	theme	cord	108:111	arg1	SCI					121:123	SCI	121:123	SCI	121:123	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	1	41	theme	cord	108:111	arg1	injury					113:118	Spinal cord injury	101:118	Spinal cord injury (SCI)	101:124	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	3	42	theme	pronounced	541:550	arg1	alterations					552:562	pronounced alterations	541:562	pronounced alterations in the extracellular proteoglycans	541:597	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	4	43	theme	reactive	679:686	arg1	astrocytes					688:697	reactive astrocytes	679:697	reactive astrocytes	679:697	These abnormalities coincided with the appearance of reactive astrocytes and a reduction in main astrocytic glutamate transporter 1.					
30953739	2	44	theme	matrix	312:317	arg1	changes					263:269	Cellular changes	254:269	Cellular changes	254:269	Cellular changes and the composition of the extracellular matrix in regions distal from the injured area remain poorly characterized.					
30953739	2	44	theme	matrix	312:317	arg1	composition					279:289	the composition	275:289	the composition of the extracellular matrix in regions distal from the injured area	275:357	Cellular changes and the composition of the extracellular matrix in regions distal from the injured area remain poorly characterized.					
30953739	1	45	theme	glial	199:203	arg1	scar					205:208	a glial scar	197:208	a glial scar that blocks axon regeneration and regrowth	197:251	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	4	46	theme	glutamate	734:742	arg1	transporter					744:754	main astrocytic glutamate transporter 1	718:756	main astrocytic glutamate transporter 1	718:756	These abnormalities coincided with the appearance of reactive astrocytes and a reduction in main astrocytic glutamate transporter 1.					
30953739	2	47	theme	extracellular	298:310	arg1	matrix					312:317	the extracellular matrix	294:317	the extracellular matrix	294:317	Cellular changes and the composition of the extracellular matrix in regions distal from the injured area remain poorly characterized.					
30953739	6	48	theme	SCI	1090:1092	arg1	aggravation					1071:1081	further aggravation	1063:1081	further aggravation of the SCI	1063:1092	The results of the study suggest that SCI induces widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI.					
30953739	6	48	theme	SCI	1090:1092	arg1	dysfunction					1021:1031	neuronal dysfunction	1012:1031	neuronal dysfunction far from the damaged area	1012:1057	The results of the study suggest that SCI induces widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI.					
30953739	1	49	theme	scar	205:208	arg1	formation					184:192	subsequent formation	173:192	subsequent formation of a glial scar that blocks axon regeneration and regrowth	173:251	Spinal cord injury (SCI) results in pronounced focal tissue damage with subsequent formation of a glial scar that blocks axon regeneration and regrowth.					
30953739	3	50	theme	distal	429:434	arg1	cord					424:427	the spinal cord	413:427	the spinal cord distal to the damaged area (perilesion perimeter)	413:477	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	2	51	theme	injured	346:352	arg1	area					354:357	the injured area	342:357	the injured area	342:357	Cellular changes and the composition of the extracellular matrix in regions distal from the injured area remain poorly characterized.					
30953739	2	52	from	changes	263:269	arg1	regions					322:328	regions	322:328	regions distal from the injured area	322:357	Cellular changes and the composition of the extracellular matrix in regions distal from the injured area remain poorly characterized.					
30953739	0	53	theme	ventral	61:67	arg1	horns					69:73	ventral horns	61:73	ventral horns	61:73	Upregulation of proteoglycans in the perilesion perimeter in ventral horns after spinal cord injury.					
30953739	3	54	theme	extracellular	571:583	arg1	proteoglycans					585:597	the extracellular proteoglycans	567:597	the extracellular proteoglycans	567:597	In the present study, in the spinal cord distal to the damaged area (perilesion perimeter) there were minimal gross histological changes, but there were pronounced alterations in the extracellular proteoglycans even at 30 days after SCI.					
30953739	5	55	theme	different	789:797	arg1	kinetics					799:806	different kinetics	789:806	different kinetics	789:806	Proteoglycan levels exhibited different kinetics and changes after SCI in areas near neuronal cell bodies and in areas distal from them.					
30953739	2	56	from	composition	279:289	arg1	regions					322:328	regions	322:328	regions distal from the injured area	322:357	Cellular changes and the composition of the extracellular matrix in regions distal from the injured area remain poorly characterized.					
30953739	6	57	theme	spinal	972:977	arg1	cord					979:982	the spinal cord	968:982	the spinal cord	968:982	The results of the study suggest that SCI induces widespread changes in the spinal cord that may be responsible for neuronal dysfunction far from the damaged area and further aggravation of the SCI.					
30075251	3	0	theme	enhancement	640:650	arg1	capability					652:661	potential bioavailability enhancement capability	614:661	potential bioavailability enhancement capability	614:661	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	6	1	used	used	958:961	arg2	parameters					979:988	the decisive parameters	966:988	the decisive parameters for formulation optimisation	966:1017	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	6	1	used	used	958:961	arg2	stability					923:931	stability	923:931	stability	923:931	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	6	1	used	used	958:961	arg2	ability					911:917	The emulsion reconstitution ability	883:917	The emulsion reconstitution ability	883:917	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	3	2	theme	formulation	488:498	arg1	development					500:510	the preliminary formulation development	472:510	the preliminary formulation development	472:510	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	7	3	theme	size	1065:1068	arg1	distribution					1070:1081	narrow droplet size distribution	1050:1081	narrow droplet size distribution	1050:1081	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	1	4	dep	lipophilic	228:237	arg1	soluble					253:259	soluble	253:259	soluble	253:259	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	1	5	theme	study	164:168	arg1	purpose					149:155	The purpose	145:155	The purpose of the study	145:168	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	8	6	theme	scanning	1236:1243	arg1	microscopy					1254:1263	The scanning electron microscopy	1232:1263	The scanning electron microscopy	1232:1263	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	7	7	theme	narrow	1050:1055	arg1	distribution					1070:1081	narrow droplet size distribution	1050:1081	narrow droplet size distribution	1050:1081	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	3	8	theme	phase	512:516	arg1	phase					569:573	the oily lipid phase	554:573	the oily lipid phase	554:573	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	3	8	theme	phase	512:516	arg1	1-oleoyl-rac-glycerol					518:538	phase 1-oleoyl-rac-glycerol	512:538	phase 1-oleoyl-rac-glycerol	512:538	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	3	9	theme	oily	558:561	arg1	phase					569:573	the oily lipid phase	554:573	the oily lipid phase	554:573	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	3	9	theme	oily	558:561	arg1	1-oleoyl-rac-glycerol					518:538	phase 1-oleoyl-rac-glycerol	512:538	phase 1-oleoyl-rac-glycerol	512:538	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	5	10	theme	mixture	802:808	arg1	approach					817:824	the mixture design approach	798:824	specifically the mixture design approach	785:824	The design of experiments, specifically the mixture design approach, was used to obtain the optimal formulation composition.					
30075251	5	10	theme	mixture	802:808	arg1	experiments					772:782	experiments	772:782	experiments	772:782	The design of experiments, specifically the mixture design approach, was used to obtain the optimal formulation composition.					
30075251	6	11	theme	reconstitution	896:909	arg1	stability					923:931	stability	923:931	stability	923:931	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	6	11	theme	reconstitution	896:909	arg1	ability					911:917	The emulsion reconstitution ability	883:917	The emulsion reconstitution ability	883:917	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	6	11	theme	reconstitution	896:909	arg1	parameters					979:988	the decisive parameters	966:988	the decisive parameters for formulation optimisation	966:1017	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	8	12	theme	Raman	1266:1270	arg1	spectroscopy					1272:1283	Raman spectroscopy	1266:1283	Raman spectroscopy	1266:1283	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	0	13	theme	lipophilic	128:137	arg1	drug					139:142	a lipophilic drug	126:142	a lipophilic drug	126:142	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	5	14	used	used	831:834	arg2	design					762:767	The design	758:767	The design of experiments, specifically the mixture design approach,	758:825	The design of experiments, specifically the mixture design approach, was used to obtain the optimal formulation composition.					
30075251	5	15	theme	formulation	858:868	arg1	composition					870:880	the optimal formulation composition	846:880	the optimal formulation composition	846:880	The design of experiments, specifically the mixture design approach, was used to obtain the optimal formulation composition.					
30075251	8	16	theme	coated	1451:1456	arg1	pellets					1458:1464	coated pellets	1451:1464	coated pellets	1451:1464	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	6	17	theme	decisive	970:977	arg1	stability					923:931	stability	923:931	stability	923:931	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	6	17	theme	decisive	970:977	arg1	ability					911:917	The emulsion reconstitution ability	883:917	The emulsion reconstitution ability	883:917	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	6	17	theme	decisive	970:977	arg1	parameters					979:988	the decisive parameters	966:988	the decisive parameters for formulation optimisation	966:1017	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	8	18	with	morphology	1324:1333	arg1	droplets					1371:1378	separated droplets	1361:1378	separated droplets with simvastatin and uniform size distribution	1361:1425	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	7	19	theme	enhanced	1146:1153	arg1	profile					1167:1173	enhanced dissolution profile	1146:1173	enhanced dissolution profile	1146:1173	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	9	20	theme	concept	1501:1507	arg1	proof					1492:1496	the proof	1488:1496	the proof of concept of designing redispersible dry emulsions using a fluid bed layering approach	1488:1584	The study represents the proof of concept of designing redispersible dry emulsions using a fluid bed layering approach.					
30075251	0	21	theme	bed	72:74	arg1	layering					76:83	fluid bed layering	66:83	fluid bed layering	66:83	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	1	22	theme	bed	304:306	arg1	technology					316:325	fluid bed coating technology	298:325	fluid bed coating technology	298:325	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	4	23	theme	solid	713:717	arg1	HPMC					674:677	HPMC	674:677	HPMC	674:677	Mannitol, HPMC and Tween 20 were selected as the solid carriers and surfactant, respectively.					
30075251	4	23	theme	solid	713:717	arg1	Mannitol					664:671	Mannitol	664:671	Mannitol	664:671	Mannitol, HPMC and Tween 20 were selected as the solid carriers and surfactant, respectively.					
30075251	4	23	theme	solid	713:717	arg1	carriers					719:726	the solid carriers	709:726	the solid carriers	709:726	Mannitol, HPMC and Tween 20 were selected as the solid carriers and surfactant, respectively.					
30075251	4	23	theme	solid	713:717	arg1	Tween					683:687	Tween	683:687	Tween	683:687	Mannitol, HPMC and Tween 20 were selected as the solid carriers and surfactant, respectively.					
30075251	9	24	theme	dry	1536:1538	arg1	emulsions					1540:1548	redispersible dry emulsions	1522:1548	redispersible dry emulsions using a fluid bed layering approach	1522:1584	The study represents the proof of concept of designing redispersible dry emulsions using a fluid bed layering approach.					
30075251	6	25	theme	main	942:945	arg1	responses					947:955	the main responses	938:955	the main responses	938:955	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	2	26	theme	neutral	455:461	arg1	core					463:466	a neutral core	453:466	a neutral core	453:466	The presented dry emulsion manufacturing approach produces pellets in a way, where a layer of the dry emulsion is applied to a neutral core.					
30075251	8	27	theme	layer	1350:1354	arg1	shape					1442:1446	a circular shape	1431:1446	a circular shape of coated pellets	1431:1464	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	8	27	theme	layer	1350:1354	arg1	morphology					1324:1333	a uniform morphology	1314:1333	a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution	1314:1425	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	2	28	theme	emulsion	346:353	arg1	approach					369:376	The presented dry emulsion manufacturing approach	328:376	The presented dry emulsion manufacturing approach	328:376	The presented dry emulsion manufacturing approach produces pellets in a way, where a layer of the dry emulsion is applied to a neutral core.					
30075251	9	29	theme	fluid	1558:1562	arg1	approach					1577:1584	a fluid bed layering approach	1556:1584	a fluid bed layering approach	1556:1584	The study represents the proof of concept of designing redispersible dry emulsions using a fluid bed layering approach.					
30075251	8	30	theme	separated	1361:1369	arg1	droplets					1371:1378	separated droplets	1361:1378	separated droplets with simvastatin and uniform size distribution	1361:1425	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	5	31	theme	experiments	772:782	arg1	design					762:767	The design	758:767	The design of experiments, specifically the mixture design approach,	758:825	The design of experiments, specifically the mixture design approach, was used to obtain the optimal formulation composition.					
30075251	9	32	theme	layering	1568:1575	arg1	approach					1577:1584	a fluid bed layering approach	1556:1584	a fluid bed layering approach	1556:1584	The study represents the proof of concept of designing redispersible dry emulsions using a fluid bed layering approach.					
30075251	2	33	theme	dry	426:428	arg1	emulsion					430:437	the dry emulsion	422:437	the dry emulsion	422:437	The presented dry emulsion manufacturing approach produces pellets in a way, where a layer of the dry emulsion is applied to a neutral core.					
30075251	3	34	theme	drug	594:597	arg1	solubility					599:608	the high drug solubility	585:608	the high drug solubility	585:608	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	0	35	theme	dry	16:18	arg1	system					29:34	A redispersible dry emulsion system	0:34	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.	0:143	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	1	36	theme	drug	267:270	arg1	simvastatin					272:282	a lipophilic, poorly water soluble model drug simvastatin	226:282	a lipophilic, poorly water soluble model drug simvastatin	226:282	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	7	37	theme	suitable	1120:1127	arg1	loading					1134:1140	suitable drug loading	1120:1140	suitable drug loading	1120:1140	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	1	38	contain	containing	215:224	arg1	emulsion					205:212	a redispersible dry emulsion	185:212	a redispersible dry emulsion	185:212	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	1	38	contain	containing	215:224	arg2	simvastatin					272:282	a lipophilic, poorly water soluble model drug simvastatin	226:282	a lipophilic, poorly water soluble model drug simvastatin	226:282	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	3	39	theme	bioavailability	624:638	arg1	capability					652:661	potential bioavailability enhancement capability	614:661	potential bioavailability enhancement capability	614:661	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	8	40	theme	size	1409:1412	arg1	distribution					1414:1425	uniform size distribution	1401:1425	uniform size distribution	1401:1425	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	1	41	theme	redispersible	187:199	arg1	emulsion					205:212	a redispersible dry emulsion	185:212	a redispersible dry emulsion	185:212	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	2	42	theme	presented	332:340	arg1	approach					369:376	The presented dry emulsion manufacturing approach	328:376	The presented dry emulsion manufacturing approach	328:376	The presented dry emulsion manufacturing approach produces pellets in a way, where a layer of the dry emulsion is applied to a neutral core.					
30075251	7	43	theme	high	1104:1107	arg1	stability					1109:1117	high stability	1104:1117	high stability	1104:1117	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	0	44	with	system	29:34	arg1	simvastatin					41:51	simvastatin	41:51	simvastatin	41:51	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	7	45	theme	pure	1221:1224	arg1	drug					1226:1229	the pure drug	1217:1229	the pure drug	1217:1229	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	3	46	theme	preliminary	476:486	arg1	development					500:510	the preliminary formulation development	472:510	the preliminary formulation development	472:510	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	1	47	theme	lipophilic	228:237	arg1	simvastatin					272:282	a lipophilic, poorly water soluble model drug simvastatin	226:282	a lipophilic, poorly water soluble model drug simvastatin	226:282	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	5	48	theme	design	810:815	arg1	approach					817:824	the mixture design approach	798:824	specifically the mixture design approach	785:824	The design of experiments, specifically the mixture design approach, was used to obtain the optimal formulation composition.					
30075251	5	48	theme	design	810:815	arg1	experiments					772:782	experiments	772:782	experiments	772:782	The design of experiments, specifically the mixture design approach, was used to obtain the optimal formulation composition.					
30075251	7	49	theme	based	1200:1204	arg1	tablet					1206:1211	a non-lipid based tablet	1188:1211	a non-lipid based tablet	1188:1211	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	7	50	theme	droplet	1057:1063	arg1	distribution					1070:1081	narrow droplet size distribution	1050:1081	narrow droplet size distribution	1050:1081	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	0	51	theme	enhancement	111:121	arg1	means					90:94	a means	88:94	a means of dissolution enhancement of a lipophilic drug	88:142	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	8	52	theme	circular	1433:1440	arg1	shape					1442:1446	a circular shape	1431:1446	a circular shape of coated pellets	1431:1464	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	8	53	with	droplets	1371:1378	arg1	simvastatin					1385:1395	simvastatin	1385:1395	simvastatin	1385:1395	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	8	53	with	droplets	1371:1378	arg1	distribution					1414:1425	uniform size distribution	1401:1425	uniform size distribution	1401:1425	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	7	54	theme	Optimised	1020:1028	arg1	formulations					1030:1041	Optimised formulations	1020:1041	Optimised formulations	1020:1041	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	8	55	theme	electron	1245:1252	arg1	microscopy					1254:1263	The scanning electron microscopy	1232:1263	The scanning electron microscopy	1232:1263	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	0	56	theme	drug	139:142	arg1	enhancement					111:121	dissolution enhancement	99:121	dissolution enhancement of a lipophilic drug	99:142	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	5	57	theme	optimal	850:856	arg1	composition					870:880	the optimal formulation composition	846:880	the optimal formulation composition	846:880	The design of experiments, specifically the mixture design approach, was used to obtain the optimal formulation composition.					
30075251	6	58	theme	emulsion	887:894	arg1	stability					923:931	stability	923:931	stability	923:931	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	6	58	theme	emulsion	887:894	arg1	ability					911:917	The emulsion reconstitution ability	883:917	The emulsion reconstitution ability	883:917	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	6	58	theme	emulsion	887:894	arg1	parameters					979:988	the decisive parameters	966:988	the decisive parameters for formulation optimisation	966:1017	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	8	59	theme	pellets	1458:1464	arg1	shape					1442:1446	a circular shape	1431:1446	a circular shape of coated pellets	1431:1464	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	8	59	theme	pellets	1458:1464	arg1	morphology					1324:1333	a uniform morphology	1314:1333	a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution	1314:1425	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	0	60	theme	fluid	66:70	arg1	layering					76:83	fluid bed layering	66:83	fluid bed layering	66:83	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	3	61	theme	lipid	563:567	arg1	phase					569:573	the oily lipid phase	554:573	the oily lipid phase	554:573	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	3	61	theme	lipid	563:567	arg1	1-oleoyl-rac-glycerol					518:538	phase 1-oleoyl-rac-glycerol	512:538	phase 1-oleoyl-rac-glycerol	512:538	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	8	62	theme	image	1289:1293	arg1	analysis					1295:1302	image analysis	1289:1302	image analysis	1289:1302	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	7	63	theme	non-lipid	1190:1198	arg1	tablet					1206:1211	a non-lipid based tablet	1188:1211	a non-lipid based tablet	1188:1211	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	1	64	theme	fluid	298:302	arg1	technology					316:325	fluid bed coating technology	298:325	fluid bed coating technology	298:325	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	2	65	theme	emulsion	430:437	arg1	layer					413:417	a layer	411:417	a layer of the dry emulsion	411:437	The presented dry emulsion manufacturing approach produces pellets in a way, where a layer of the dry emulsion is applied to a neutral core.					
30075251	6	66	dep	used	958:961	arg1	responses					947:955	the main responses	938:955	the main responses	938:955	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	1	67	theme	coating	308:314	arg1	technology					316:325	fluid bed coating technology	298:325	fluid bed coating technology	298:325	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	9	68	theme	redispersible	1522:1534	arg1	emulsions					1540:1548	redispersible dry emulsions	1522:1548	redispersible dry emulsions using a fluid bed layering approach	1522:1584	The study represents the proof of concept of designing redispersible dry emulsions using a fluid bed layering approach.					
30075251	8	69	theme	uniform	1316:1322	arg1	morphology					1324:1333	a uniform morphology	1314:1333	a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution	1314:1425	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	2	70	theme	manufacturing	355:367	arg1	approach					369:376	The presented dry emulsion manufacturing approach	328:376	The presented dry emulsion manufacturing approach	328:376	The presented dry emulsion manufacturing approach produces pellets in a way, where a layer of the dry emulsion is applied to a neutral core.					
30075251	0	71	theme	redispersible	2:14	arg1	system					29:34	A redispersible dry emulsion system	0:34	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.	0:143	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	8	72	theme	applied	1342:1348	arg1	layer					1350:1354	the applied layer	1338:1354	the applied layer	1338:1354	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	2	73	theme	dry	342:344	arg1	approach					369:376	The presented dry emulsion manufacturing approach	328:376	The presented dry emulsion manufacturing approach	328:376	The presented dry emulsion manufacturing approach produces pellets in a way, where a layer of the dry emulsion is applied to a neutral core.					
30075251	0	74	theme	emulsion	20:27	arg1	system					29:34	A redispersible dry emulsion system	0:34	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.	0:143	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	9	75	theme	bed	1564:1566	arg1	approach					1577:1584	a fluid bed layering approach	1556:1584	a fluid bed layering approach	1556:1584	The study represents the proof of concept of designing redispersible dry emulsions using a fluid bed layering approach.					
30075251	3	76	theme	high	589:592	arg1	solubility					599:608	the high drug solubility	585:608	the high drug solubility	585:608	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	7	77	theme	drug	1129:1132	arg1	loading					1134:1140	suitable drug loading	1120:1140	suitable drug loading	1120:1140	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
30075251	1	78	theme	model	261:265	arg1	simvastatin					272:282	a lipophilic, poorly water soluble model drug simvastatin	226:282	a lipophilic, poorly water soluble model drug simvastatin	226:282	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	6	79	theme	formulation	994:1004	arg1	optimisation					1006:1017	formulation optimisation	994:1017	formulation optimisation	994:1017	The emulsion reconstitution ability and stability were the main responses, used as the decisive parameters for formulation optimisation.					
30075251	3	80	theme	potential	614:622	arg1	capability					652:661	potential bioavailability enhancement capability	614:661	potential bioavailability enhancement capability	614:661	In the preliminary formulation development phase 1-oleoyl-rac-glycerol was chosen as the oily lipid phase, based on the high drug solubility and potential bioavailability enhancement capability.					
30075251	8	81	with	shape	1442:1446	arg1	droplets					1371:1378	separated droplets	1361:1378	separated droplets with simvastatin and uniform size distribution	1361:1425	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	8	82	theme	uniform	1401:1407	arg1	distribution					1414:1425	uniform size distribution	1401:1425	uniform size distribution	1401:1425	The scanning electron microscopy, Raman spectroscopy and image analysis disclosed a uniform morphology of the applied layer with separated droplets with simvastatin and uniform size distribution and a circular shape of coated pellets.					
30075251	1	83	theme	dry	201:203	arg1	emulsion					205:212	a redispersible dry emulsion	185:212	a redispersible dry emulsion	185:212	The purpose of the study was to develop a redispersible dry emulsion, containing a lipophilic, poorly water soluble model drug simvastatin, by employing fluid bed coating technology.					
30075251	0	84	theme	dissolution	99:109	arg1	enhancement					111:121	dissolution enhancement	99:121	dissolution enhancement of a lipophilic drug	99:142	A redispersible dry emulsion system with simvastatin prepared via fluid bed layering as a means of dissolution enhancement of a lipophilic drug.					
30075251	7	85	theme	dissolution	1155:1165	arg1	profile					1167:1173	enhanced dissolution profile	1146:1173	enhanced dissolution profile	1146:1173	Optimised formulations showed narrow droplet size distribution upon reconstitution, high stability, suitable drug loading and enhanced dissolution profile, compared to a non-lipid based tablet and the pure drug.					
29842958	5	0	theme	nanospheroid	614:625	arg1	morphology					648:657	nanospheroid and nanopaddy shaped morphology	614:657	nanospheroid and nanopaddy shaped morphology	614:657	Pure ZnO and Agar/ZnO nanocomposites showed nanospheroid and nanopaddy shaped morphology from FESEM studies.					
29842958	10	1	theme	ZnO	1074:1076	arg1	photoluminescence					1053:1069	Oxygen vacancy induced photoluminescence	1030:1069	Oxygen vacancy induced photoluminescence of ZnO	1030:1076	Oxygen vacancy induced photoluminescence of ZnO are observed and its intensity is found to be increased linearly with the Agar concentration.					
29842958	0	2	theme	ZnO	69:71	arg1	properties					50:59	Structural, morphological, optical and biological properties	0:59	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.	0:107	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.					
29842958	10	3	theme	induced	1045:1051	arg1	photoluminescence					1053:1069	Oxygen vacancy induced photoluminescence	1030:1069	Oxygen vacancy induced photoluminescence of ZnO	1030:1076	Oxygen vacancy induced photoluminescence of ZnO are observed and its intensity is found to be increased linearly with the Agar concentration.					
29842958	11	4	dep	Gram-negative	1312:1324	arg1	aeruginosa					1330:1339	P. aeruginosa	1327:1339	P. aeruginosa	1327:1339	The antibacterial activity of ZnO and Agar/ZnO nanocomposites was evaluated by disc diffusion method against Gram-positive (B.subtilis) and Gram-negative (P. aeruginosa) bacteria.					
29842958	11	5	theme	diffusion	1256:1264	arg1	method					1266:1271	disc diffusion method	1251:1271	disc diffusion method	1251:1271	The antibacterial activity of ZnO and Agar/ZnO nanocomposites was evaluated by disc diffusion method against Gram-positive (B.subtilis) and Gram-negative (P. aeruginosa) bacteria.					
29842958	0	6	theme	oxide	87:91	arg1	nanocomposites					93:106	agar/zinc oxide nanocomposites	77:106	agar/zinc oxide nanocomposites	77:106	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.					
29842958	4	7	theme	ZnO	488:490	arg1	size					475:478	The crystallite size	459:478	The crystallite size of pure ZnO and Agar/ZnO nanocomposites	459:518	The crystallite size of pure ZnO and Agar/ZnO nanocomposites was found to be in the range of 35.5 to 19.73 nm.					
29842958	11	8	theme	disc	1251:1254	arg1	method					1266:1271	disc diffusion method	1251:1271	disc diffusion method	1251:1271	The antibacterial activity of ZnO and Agar/ZnO nanocomposites was evaluated by disc diffusion method against Gram-positive (B.subtilis) and Gram-negative (P. aeruginosa) bacteria.					
29842958	8	9	dep	ZnO	878:880	arg1	nanocomposites					895:908	nanocomposites	895:908	nanocomposites	895:908	The optical properties of Pure ZnO and Agar/ZnO nanocomposites were characterized by UV, FTIR and PL.					
29842958	9	10	theme	nanocomposites	974:987	arg1	gap					958:960	The band gap	949:960	The band gap of Agar/ZnO nanocomposites	949:987	The band gap of Agar/ZnO nanocomposites were varied with the Agar concentration.					
29842958	13	11	theme	dependent	1556:1564	arg1	toxicity					1566:1573	a dose dependent toxicity	1549:1573	a dose dependent toxicity in normal and cancer cell line	1549:1604	The result of this investigation reveals that the Agar/ZnO nanocomposites deliver a dose dependent toxicity in normal and cancer cell line.					
29842958	0	12	theme	agar/zinc	77:85	arg1	nanocomposites					93:106	agar/zinc oxide nanocomposites	77:106	agar/zinc oxide nanocomposites	77:106	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.					
29842958	11	13	theme	ZnO	1202:1204	arg1	activity					1190:1197	The antibacterial activity	1172:1197	The antibacterial activity of ZnO and Agar/ZnO nanocomposites	1172:1232	The antibacterial activity of ZnO and Agar/ZnO nanocomposites was evaluated by disc diffusion method against Gram-positive (B.subtilis) and Gram-negative (P. aeruginosa) bacteria.					
29842958	5	14	theme	shaped	641:646	arg1	morphology					648:657	nanospheroid and nanopaddy shaped morphology	614:657	nanospheroid and nanopaddy shaped morphology	614:657	Pure ZnO and Agar/ZnO nanocomposites showed nanospheroid and nanopaddy shaped morphology from FESEM studies.					
29842958	5	15	theme	Pure	570:573	arg1	nanocomposites					592:605	Pure ZnO and Agar/ZnO nanocomposites	570:605	Pure ZnO and Agar/ZnO nanocomposites	570:605	Pure ZnO and Agar/ZnO nanocomposites showed nanospheroid and nanopaddy shaped morphology from FESEM studies.					
29842958	12	16	theme	nanocomposites	1381:1394	arg1	cytotoxicity					1356:1367	The cytotoxicity	1352:1367	The cytotoxicity of Agar/ZnO nanocomposites	1352:1394	The cytotoxicity of Agar/ZnO nanocomposites was studied against Normal (L929) and Breast cancer cell line (MB231).					
29842958	8	17	theme	optical	851:857	arg1	properties					859:868	The optical properties	847:868	The optical properties of Pure ZnO and Agar/ZnO nanocomposites	847:908	The optical properties of Pure ZnO and Agar/ZnO nanocomposites were characterized by UV, FTIR and PL.					
29842958	5	18	theme	ZnO	575:577	arg1	nanocomposites					592:605	Pure ZnO and Agar/ZnO nanocomposites	570:605	Pure ZnO and Agar/ZnO nanocomposites	570:605	Pure ZnO and Agar/ZnO nanocomposites showed nanospheroid and nanopaddy shaped morphology from FESEM studies.					
29842958	2	19	theme	concentrations	254:267	arg1	influence					236:244	The influence	232:244	The influence of Agar concentrations on the structural, morphological and optical properties of ZnO	232:330	The influence of Agar concentrations on the structural, morphological and optical properties of ZnO have been investigated.					
29842958	6	20	theme	interplanar	683:693	arg1	distance					695:702	The interplanar distance	679:702	The interplanar distance observed from the HRTEM image	679:732	The interplanar distance observed from the HRTEM image confirms the plane of the prepared material.					
29842958	0	21	theme	nanocomposites	93:106	arg1	properties					50:59	Structural, morphological, optical and biological properties	0:59	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.	0:107	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.					
29842958	3	22	dep	ZnO	380:382	arg1	nanocomposites					397:410	nanocomposites	397:410	nanocomposites	397:410	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites indicates the hexagonal wurtzite phase of ZnO.					
29842958	6	23	theme	material	769:776	arg1	plane					747:751	the plane	743:751	the plane of the prepared material	743:776	The interplanar distance observed from the HRTEM image confirms the plane of the prepared material.					
29842958	2	24	theme	Agar	249:252	arg1	concentrations					254:267	Agar concentrations	249:267	Agar concentrations	249:267	The influence of Agar concentrations on the structural, morphological and optical properties of ZnO have been investigated.					
29842958	9	25	theme	band	953:956	arg1	gap					958:960	The band gap	949:960	The band gap of Agar/ZnO nanocomposites	949:987	The band gap of Agar/ZnO nanocomposites were varied with the Agar concentration.					
29842958	1	26	theme	in	158:159	arg1	method					189:194	in situ chemical precipitation method	158:194	in situ chemical precipitation method	158:194	In this work, ZnO nanoparticles were prepared by in situ chemical precipitation method in the presence of Agar biopolymer.					
29842958	4	27	theme	crystallite	463:473	arg1	size					475:478	The crystallite size	459:478	The crystallite size of pure ZnO and Agar/ZnO nanocomposites	459:518	The crystallite size of pure ZnO and Agar/ZnO nanocomposites was found to be in the range of 35.5 to 19.73 nm.					
29842958	5	28	theme	Agar/ZnO	583:590	arg1	nanocomposites					592:605	Pure ZnO and Agar/ZnO nanocomposites	570:605	Pure ZnO and Agar/ZnO nanocomposites	570:605	Pure ZnO and Agar/ZnO nanocomposites showed nanospheroid and nanopaddy shaped morphology from FESEM studies.					
29842958	3	29	theme	ZnO	380:382	arg1	pattern					364:370	The XRD pattern	356:370	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites	356:410	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites indicates the hexagonal wurtzite phase of ZnO.					
29842958	0	30	theme	Structural	0:9	arg1	properties					50:59	Structural, morphological, optical and biological properties	0:59	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.	0:107	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.					
29842958	7	31	theme	elemental	783:791	arg1	composition					793:803	The elemental composition	779:803	The elemental composition of the samples	779:818	The elemental composition of the samples were characterized by EDX.					
29842958	10	32	theme	vacancy	1037:1043	arg1	photoluminescence					1053:1069	Oxygen vacancy induced photoluminescence	1030:1069	Oxygen vacancy induced photoluminescence of ZnO	1030:1076	Oxygen vacancy induced photoluminescence of ZnO are observed and its intensity is found to be increased linearly with the Agar concentration.					
29842958	2	33	theme	morphological	288:300	arg1	properties					314:323	the structural, morphological and optical properties	272:323	the structural, morphological and optical properties of ZnO	272:330	The influence of Agar concentrations on the structural, morphological and optical properties of ZnO have been investigated.					
29842958	7	34	theme	samples	812:818	arg1	composition					793:803	The elemental composition	779:803	The elemental composition of the samples	779:818	The elemental composition of the samples were characterized by EDX.					
29842958	1	35	theme	chemical	166:173	arg1	method					189:194	in situ chemical precipitation method	158:194	in situ chemical precipitation method	158:194	In this work, ZnO nanoparticles were prepared by in situ chemical precipitation method in the presence of Agar biopolymer.					
29842958	4	36	dep	ZnO	488:490	arg1	nanocomposites					505:518	nanocomposites	505:518	nanocomposites	505:518	The crystallite size of pure ZnO and Agar/ZnO nanocomposites was found to be in the range of 35.5 to 19.73 nm.					
29842958	13	37	from	toxicity	1566:1573	arg1	normal					1578:1583	normal	1578:1583	normal	1578:1583	The result of this investigation reveals that the Agar/ZnO nanocomposites deliver a dose dependent toxicity in normal and cancer cell line.					
29842958	13	37	from	toxicity	1566:1573	arg1	line					1601:1604	cancer cell line	1589:1604	cancer cell line	1589:1604	The result of this investigation reveals that the Agar/ZnO nanocomposites deliver a dose dependent toxicity in normal and cancer cell line.					
29842958	12	38	theme	Agar/ZnO	1372:1379	arg1	nanocomposites					1381:1394	Agar/ZnO nanocomposites	1372:1394	Agar/ZnO nanocomposites	1372:1394	The cytotoxicity of Agar/ZnO nanocomposites was studied against Normal (L929) and Breast cancer cell line (MB231).					
29842958	1	39	theme	precipitation	175:187	arg1	method					189:194	in situ chemical precipitation method	158:194	in situ chemical precipitation method	158:194	In this work, ZnO nanoparticles were prepared by in situ chemical precipitation method in the presence of Agar biopolymer.					
29842958	0	40	theme	morphological	12:24	arg1	properties					50:59	Structural, morphological, optical and biological properties	0:59	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.	0:107	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.					
29842958	11	41	theme	Agar/ZnO	1210:1217	arg1	activity					1190:1197	The antibacterial activity	1172:1197	The antibacterial activity of ZnO and Agar/ZnO nanocomposites	1172:1232	The antibacterial activity of ZnO and Agar/ZnO nanocomposites was evaluated by disc diffusion method against Gram-positive (B.subtilis) and Gram-negative (P. aeruginosa) bacteria.					
29842958	2	42	theme	structural	276:285	arg1	properties					314:323	the structural, morphological and optical properties	272:323	the structural, morphological and optical properties of ZnO	272:330	The influence of Agar concentrations on the structural, morphological and optical properties of ZnO have been investigated.					
29842958	8	43	theme	Agar/ZnO	886:893	arg1	properties					859:868	The optical properties	847:868	The optical properties of Pure ZnO and Agar/ZnO nanocomposites	847:908	The optical properties of Pure ZnO and Agar/ZnO nanocomposites were characterized by UV, FTIR and PL.					
29842958	3	44	theme	ZnO	454:456	arg1	phase					445:449	the hexagonal wurtzite phase	422:449	the hexagonal wurtzite phase of ZnO	422:456	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites indicates the hexagonal wurtzite phase of ZnO.					
29842958	11	45	dep	Gram-positive	1281:1293	arg1	bacteria					1342:1349	bacteria	1342:1349	bacteria	1342:1349	The antibacterial activity of ZnO and Agar/ZnO nanocomposites was evaluated by disc diffusion method against Gram-positive (B.subtilis) and Gram-negative (P. aeruginosa) bacteria.					
29842958	2	46	from	influence	236:244	arg1	properties					314:323	the structural, morphological and optical properties	272:323	the structural, morphological and optical properties of ZnO	272:330	The influence of Agar concentrations on the structural, morphological and optical properties of ZnO have been investigated.					
29842958	3	47	theme	wurtzite	436:443	arg1	phase					445:449	the hexagonal wurtzite phase	422:449	the hexagonal wurtzite phase of ZnO	422:456	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites indicates the hexagonal wurtzite phase of ZnO.					
29842958	0	48	theme	optical	27:33	arg1	properties					50:59	Structural, morphological, optical and biological properties	0:59	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.	0:107	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.					
29842958	12	49	theme	cell	1448:1451	arg1	MB231					1459:1463	MB231	1459:1463	MB231	1459:1463	The cytotoxicity of Agar/ZnO nanocomposites was studied against Normal (L929) and Breast cancer cell line (MB231).					
29842958	12	49	theme	cell	1448:1451	arg1	line					1453:1456	Breast cancer cell line	1434:1456	Breast cancer cell line (MB231)	1434:1464	The cytotoxicity of Agar/ZnO nanocomposites was studied against Normal (L929) and Breast cancer cell line (MB231).					
29842958	3	50	theme	Agar/ZnO	388:395	arg1	pattern					364:370	The XRD pattern	356:370	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites	356:410	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites indicates the hexagonal wurtzite phase of ZnO.					
29842958	9	51	theme	Agar/ZnO	965:972	arg1	nanocomposites					974:987	Agar/ZnO nanocomposites	965:987	Agar/ZnO nanocomposites	965:987	The band gap of Agar/ZnO nanocomposites were varied with the Agar concentration.					
29842958	4	52	theme	Agar/ZnO	496:503	arg1	size					475:478	The crystallite size	459:478	The crystallite size of pure ZnO and Agar/ZnO nanocomposites	459:518	The crystallite size of pure ZnO and Agar/ZnO nanocomposites was found to be in the range of 35.5 to 19.73 nm.					
29842958	13	53	theme	cell	1596:1599	arg1	line					1601:1604	cancer cell line	1589:1604	cancer cell line	1589:1604	The result of this investigation reveals that the Agar/ZnO nanocomposites deliver a dose dependent toxicity in normal and cancer cell line.					
29842958	11	54	theme	antibacterial	1176:1188	arg1	activity					1190:1197	The antibacterial activity	1172:1197	The antibacterial activity of ZnO and Agar/ZnO nanocomposites	1172:1232	The antibacterial activity of ZnO and Agar/ZnO nanocomposites was evaluated by disc diffusion method against Gram-positive (B.subtilis) and Gram-negative (P. aeruginosa) bacteria.					
29842958	2	55	theme	ZnO	328:330	arg1	properties					314:323	the structural, morphological and optical properties	272:323	the structural, morphological and optical properties of ZnO	272:330	The influence of Agar concentrations on the structural, morphological and optical properties of ZnO have been investigated.					
29842958	0	56	theme	biological	39:48	arg1	properties					50:59	Structural, morphological, optical and biological properties	0:59	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.	0:107	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.					
29842958	10	57	theme	Oxygen	1030:1035	arg1	photoluminescence					1053:1069	Oxygen vacancy induced photoluminescence	1030:1069	Oxygen vacancy induced photoluminescence of ZnO	1030:1076	Oxygen vacancy induced photoluminescence of ZnO are observed and its intensity is found to be increased linearly with the Agar concentration.					
29842958	1	58	dep	in	158:159	arg1	situ					161:164	situ	161:164	situ	161:164	In this work, ZnO nanoparticles were prepared by in situ chemical precipitation method in the presence of Agar biopolymer.					
29842958	6	59	theme	prepared	760:767	arg1	material					769:776	the prepared material	756:776	the prepared material	756:776	The interplanar distance observed from the HRTEM image confirms the plane of the prepared material.					
29842958	5	60	theme	FESEM	664:668	arg1	studies					670:676	FESEM studies	664:676	FESEM studies	664:676	Pure ZnO and Agar/ZnO nanocomposites showed nanospheroid and nanopaddy shaped morphology from FESEM studies.					
29842958	12	61	theme	cancer	1441:1446	arg1	MB231					1459:1463	MB231	1459:1463	MB231	1459:1463	The cytotoxicity of Agar/ZnO nanocomposites was studied against Normal (L929) and Breast cancer cell line (MB231).					
29842958	12	61	theme	cancer	1441:1446	arg1	line					1453:1456	Breast cancer cell line	1434:1456	Breast cancer cell line (MB231)	1434:1464	The cytotoxicity of Agar/ZnO nanocomposites was studied against Normal (L929) and Breast cancer cell line (MB231).					
29842958	10	62	theme	Agar	1152:1155	arg1	concentration					1157:1169	the Agar concentration	1148:1169	the Agar concentration	1148:1169	Oxygen vacancy induced photoluminescence of ZnO are observed and its intensity is found to be increased linearly with the Agar concentration.					
29842958	13	63	theme	cancer	1589:1594	arg1	line					1601:1604	cancer cell line	1589:1604	cancer cell line	1589:1604	The result of this investigation reveals that the Agar/ZnO nanocomposites deliver a dose dependent toxicity in normal and cancer cell line.					
29842958	13	64	theme	investigation	1486:1498	arg1	result					1471:1476	The result	1467:1476	The result of this investigation	1467:1498	The result of this investigation reveals that the Agar/ZnO nanocomposites deliver a dose dependent toxicity in normal and cancer cell line.					
29842958	6	65	theme	HRTEM	722:726	arg1	image					728:732	the HRTEM image	718:732	the HRTEM image	718:732	The interplanar distance observed from the HRTEM image confirms the plane of the prepared material.					
29842958	2	66	theme	optical	306:312	arg1	properties					314:323	the structural, morphological and optical properties	272:323	the structural, morphological and optical properties of ZnO	272:330	The influence of Agar concentrations on the structural, morphological and optical properties of ZnO have been investigated.					
29842958	4	67	theme	19.73 nm	560:567	arg1	range					543:547	the range	539:547	the range of 35.5 to 19.73 nm	539:567	The crystallite size of pure ZnO and Agar/ZnO nanocomposites was found to be in the range of 35.5 to 19.73 nm.					
29842958	0	68	theme	pure	64:67	arg1	ZnO					69:71	pure ZnO	64:71	pure ZnO	64:71	Structural, morphological, optical and biological properties of pure ZnO and agar/zinc oxide nanocomposites.					
29842958	4	69	dep	19.73 nm	560:567	arg1	to					557:558	to	557:558	to	557:558	The crystallite size of pure ZnO and Agar/ZnO nanocomposites was found to be in the range of 35.5 to 19.73 nm.					
29842958	8	70	theme	ZnO	878:880	arg1	properties					859:868	The optical properties	847:868	The optical properties of Pure ZnO and Agar/ZnO nanocomposites	847:908	The optical properties of Pure ZnO and Agar/ZnO nanocomposites were characterized by UV, FTIR and PL.					
29842958	1	71	theme	ZnO	123:125	arg1	nanoparticles					127:139	ZnO nanoparticles	123:139	ZnO nanoparticles	123:139	In this work, ZnO nanoparticles were prepared by in situ chemical precipitation method in the presence of Agar biopolymer.					
29842958	3	72	theme	hexagonal	426:434	arg1	phase					445:449	the hexagonal wurtzite phase	422:449	the hexagonal wurtzite phase of ZnO	422:456	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites indicates the hexagonal wurtzite phase of ZnO.					
29842958	1	73	theme	Agar	215:218	arg1	biopolymer					220:229	Agar biopolymer	215:229	Agar biopolymer	215:229	In this work, ZnO nanoparticles were prepared by in situ chemical precipitation method in the presence of Agar biopolymer.					
29842958	11	74	dep	ZnO	1202:1204	arg1	nanocomposites					1219:1232	nanocomposites	1219:1232	nanocomposites	1219:1232	The antibacterial activity of ZnO and Agar/ZnO nanocomposites was evaluated by disc diffusion method against Gram-positive (B.subtilis) and Gram-negative (P. aeruginosa) bacteria.					
29842958	12	75	theme	Breast	1434:1439	arg1	MB231					1459:1463	MB231	1459:1463	MB231	1459:1463	The cytotoxicity of Agar/ZnO nanocomposites was studied against Normal (L929) and Breast cancer cell line (MB231).					
29842958	12	75	theme	Breast	1434:1439	arg1	line					1453:1456	Breast cancer cell line	1434:1456	Breast cancer cell line (MB231)	1434:1464	The cytotoxicity of Agar/ZnO nanocomposites was studied against Normal (L929) and Breast cancer cell line (MB231).					
29842958	3	76	theme	XRD	360:362	arg1	pattern					364:370	The XRD pattern	356:370	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites	356:410	The XRD pattern of Pure ZnO and Agar/ZnO nanocomposites indicates the hexagonal wurtzite phase of ZnO.					
29842958	9	77	theme	Agar	1010:1013	arg1	concentration					1015:1027	the Agar concentration	1006:1027	the Agar concentration	1006:1027	The band gap of Agar/ZnO nanocomposites were varied with the Agar concentration.					
29842958	1	78	theme	biopolymer	220:229	arg1	presence					203:210	the presence	199:210	the presence of Agar biopolymer	199:229	In this work, ZnO nanoparticles were prepared by in situ chemical precipitation method in the presence of Agar biopolymer.					
30858293	7	0	theme	Caulobacterales	1505:1519	arg1	holdfasts					1521:1529	marine Caulobacterales holdfasts	1498:1529	marine Caulobacterales holdfasts	1498:1529	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	5	1	from	compositions	820:831	arg1	Caulobacterales					858:872	marine Caulobacterales	851:872	marine Caulobacterales	851:872	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	4	2	theme	opposite	585:592	arg1	pole					580:583	the cell pole opposite the reproductive stalk	571:615	the cell pole opposite the reproductive stalk	571:615	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	10	3	theme	strength	2262:2269	arg1	effect					2246:2251	the effect	2242:2251	the effect of ionic strength on adhesion	2242:2281	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	4	4	theme	reproductive	598:609	arg1	stalk					611:615	the reproductive stalk	594:615	the cell pole opposite the reproductive stalk	571:615	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	10	5	theme	excellent	2217:2225	arg1	model					2227:2231	an excellent model	2214:2231	an excellent model to study the effect of ionic strength on adhesion	2214:2281	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	10	5	theme	excellent	2217:2225	arg1	holdfast					2202:2209	the H. baltica holdfast	2187:2209	the H. baltica holdfast	2187:2209	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	7	6	theme	ionic	1438:1442	arg1	strength					1444:1451	higher ionic strength	1431:1451	higher ionic strength	1431:1451	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	8	7	theme	any	1798:1800	arg1	surface					1802:1808	virtually any surface	1788:1808	virtually any surface	1788:1808	Bacteria can colonize virtually any surface, and therefore, they have adapted to bind efficiently in very different environments.					
30858293	6	8	theme	different	1245:1253	arg1	compositions					1264:1275	different chemical compositions	1245:1275	different chemical compositions	1245:1275	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	9	9	dep	show	2047:2050	arg1	compare					1914:1920	compare	1914:1920	compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica	1914:2042	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	5	10	theme	H.	887:888	arg1	baltica					890:896	H. baltica	887:896	H. baltica	887:896	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	5	10	theme	H.	887:888	arg1	model					903:907	a model	901:907	a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera	901:1139	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	7	11	theme	baltica	1404:1410	arg1	holdfasts					1412:1420	H. baltica holdfasts	1401:1420	H. baltica holdfasts	1401:1420	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	10	12	theme	baltica	2194:2200	arg1	model					2227:2231	an excellent model	2214:2231	an excellent model to study the effect of ionic strength on adhesion	2214:2281	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	10	12	theme	baltica	2194:2200	arg1	holdfast					2202:2209	the H. baltica holdfast	2187:2209	the H. baltica holdfast	2187:2209	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	5	13	theme	marine	948:953	arg1	Caulobacterales					955:969	marine Caulobacterales	948:969	marine Caulobacterales	948:969	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	5	14	theme	marine	851:856	arg1	Caulobacterales					858:872	marine Caulobacterales	851:872	marine Caulobacterales	851:872	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	10	15	theme	marine	2370:2375	arg1	environment					2377:2387	the marine environment	2366:2387	the marine environment	2366:2387	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	10	16	from	effect	2246:2251	arg1	adhesion					2274:2281	adhesion	2274:2281	adhesion	2274:2281	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	1	17	theme	shear	207:211	arg1	forces					213:218	shear forces	207:218	shear forces	207:218	Bacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces.					
30858293	9	18	theme	marine	2016:2021	arg1	relative					2002:2009	a relative	2000:2009	a relative	2000:2009	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	9	18	theme	marine	2016:2021	arg1	baltica					2036:2042	the marine bacterium H. baltica	2012:2042	the marine bacterium H. baltica	2012:2042	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	6	19	theme	monosaccharide	1328:1341	arg1	residues					1343:1350	N-acetylglucosamine and galactose monosaccharide residues	1294:1350	N-acetylglucosamine and galactose monosaccharide residues	1294:1350	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	9	20	theme	H.	2033:2034	arg1	relative					2002:2009	a relative	2000:2009	a relative	2000:2009	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	9	20	theme	H.	2033:2034	arg1	baltica					2036:2042	the marine bacterium H. baltica	2012:2042	the marine bacterium H. baltica	2012:2042	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	9	21	contain	have	2078:2081	arg1	holdfasts					2068:2076	H. baltica holdfasts	2057:2076	H. baltica holdfasts	2057:2076	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	9	21	contain	have	2078:2081	arg2	morphology					2095:2104	a different morphology	2083:2104	a different morphology	2083:2104	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	9	21	contain	have	2078:2081	arg2	composition					2119:2129	chemical composition	2110:2129	chemical composition	2110:2129	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	7	22	dep	those	1458:1462	arg1	conclude					1484:1491	conclude	1484:1491	conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms	1484:1763	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	1	23	theme	environmental	139:151	arg1	pH					186:187	pH	186:187	pH	186:187	Bacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces.					
30858293	1	23	theme	environmental	139:151	arg1	forces					213:218	shear forces	207:218	shear forces	207:218	Bacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces.					
30858293	1	23	theme	environmental	139:151	arg1	factors					153:159	environmental factors	139:159	environmental factors	139:159	Bacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces.					
30858293	1	23	theme	environmental	139:151	arg1	strength					176:183	ionic strength	170:183	ionic strength	170:183	Bacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces.					
30858293	1	23	theme	environmental	139:151	arg1	temperature					190:200	temperature	190:200	temperature	190:200	Bacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces.					
30858293	5	24	theme	Caulobacterales	768:782	arg1	member					754:759	a freshwater member	741:759	a freshwater member of the Caulobacterales	741:782	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	5	24	theme	Caulobacterales	768:782	arg1	crescentus					729:738	Caulobacter crescentus	717:738	Caulobacter crescentus	717:738	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	2	25	theme	marine	232:237	arg1	bacteria					239:246	marine bacteria	232:246	marine bacteria	232:246	Therefore, marine bacteria must have developed adhesins with different compositions and structures than those of their freshwater counterparts to adapt to their natural environment.					
30858293	7	26	theme	large	1654:1658	arg1	part					1660:1663	a large part	1652:1663	a large part of their life spans attached to surfaces	1652:1704	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	0	27	theme	Caulobacterales	79:93	arg1	Adhesins					95:102	Marine Caulobacterales Adhesins	72:102	Marine Caulobacterales Adhesins	72:102	Comparative Analysis of Ionic Strength Tolerance between Freshwater and Marine Caulobacterales Adhesins.					
30858293	8	28	theme	different	1872:1880	arg1	environments					1882:1893	very different environments	1867:1893	very different environments	1867:1893	Bacteria can colonize virtually any surface, and therefore, they have adapted to bind efficiently in very different environments.					
30858293	1	29	theme	ionic	170:174	arg1	strength					176:183	ionic strength	170:183	ionic strength	170:183	Bacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces.					
30858293	4	30	theme	polar	531:535	arg1	holdfast					550:557	the holdfast	546:557	the holdfast	546:557	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	4	30	theme	polar	531:535	arg1	adhesin					537:543	a polar adhesin	529:543	a polar adhesin	529:543	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	9	31	theme	baltica	2060:2066	arg1	holdfasts					2068:2076	H. baltica holdfasts	2057:2076	H. baltica holdfasts	2057:2076	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	5	32	theme	freshwater	743:752	arg1	member					754:759	a freshwater member	741:759	a freshwater member of the Caulobacterales	741:782	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	5	32	theme	freshwater	743:752	arg1	crescentus					729:738	Caulobacter crescentus	717:738	Caulobacter crescentus	717:738	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	7	33	theme	environments.IMPORTANCE	1608:1630	arg1	bacteria					1637:1644	high-ionic-strength environments.IMPORTANCE Most bacteria	1588:1644	high-ionic-strength environments.IMPORTANCE Most bacteria	1588:1644	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	4	34	from	pole	580:583	arg1	located					560:566	located	560:566	located	560:566	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	7	35	dep	life	1674:1677	arg1	spans					1679:1683	spans	1679:1683	spans attached to surfaces	1679:1704	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	6	36	contain	contain	1286:1292	arg1	they					1281:1284	they	1281:1284	they	1281:1284	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	6	36	contain	contain	1286:1292	arg2	proteins					1356:1363	proteins	1356:1363	proteins	1356:1363	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	6	36	contain	contain	1286:1292	arg2	residues					1343:1350	N-acetylglucosamine and galactose monosaccharide residues	1294:1350	N-acetylglucosamine and galactose monosaccharide residues	1294:1350	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	9	37	theme	different	2085:2093	arg1	morphology					2095:2104	a different morphology	2083:2104	a different morphology	2083:2104	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	2	38	theme	natural	382:388	arg1	environment					390:400	their natural environment	376:400	their natural environment	376:400	Therefore, marine bacteria must have developed adhesins with different compositions and structures than those of their freshwater counterparts to adapt to their natural environment.					
30858293	0	39	theme	Comparative	0:10	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Ionic Strength	0:37	Comparative Analysis of Ionic Strength Tolerance between Freshwater and Marine Caulobacterales Adhesins.					
30858293	1	40	theme	Bacterial	105:113	arg1	adhesion					115:122	Bacterial adhesion	105:122	Bacterial adhesion	105:122	Bacterial adhesion is affected by environmental factors, such as ionic strength, pH, temperature, and shear forces.					
30858293	7	41	theme	multicellular	1723:1735	arg1	biofilms					1756:1763	complex multicellular communities called biofilms	1715:1763	complex multicellular communities called biofilms	1715:1763	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	4	42	theme	located	560:566	arg1	holdfast					550:557	the holdfast	546:557	the holdfast	546:557	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	4	42	theme	located	560:566	arg1	adhesin					537:543	a polar adhesin	529:543	a polar adhesin	529:543	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	5	43	from	species	1115:1121	arg1	genera					1134:1139	the two genera	1126:1139	the two genera	1126:1139	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	3	44	theme	alphaproteobacterium	417:436	arg1	bacterium					475:483	a marine budding bacterium	458:483	a marine budding bacterium in the clade CaulobacteralesH	458:513	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	3	44	theme	alphaproteobacterium	417:436	arg1	baltica					447:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	6	45	contain	have	1240:1243	arg1	holdfasts					1230:1238	the holdfasts	1226:1238	the holdfasts	1226:1238	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	6	45	contain	have	1240:1243	arg2	compositions					1264:1275	different chemical compositions	1245:1275	different chemical compositions	1245:1275	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	3	46	theme	budding	467:473	arg1	bacterium					475:483	a marine budding bacterium	458:483	a marine budding bacterium in the clade CaulobacteralesH	458:513	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	3	46	theme	budding	467:473	arg1	baltica					447:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	0	47	theme	Strength	30:37	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Ionic Strength	0:37	Comparative Analysis of Ionic Strength Tolerance between Freshwater and Marine Caulobacterales Adhesins.					
30858293	3	48	from	bacterium	475:483	arg1	CaulobacteralesH					498:513	the clade CaulobacteralesH	488:513	the clade CaulobacteralesH	488:513	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	2	49	theme	freshwater	340:349	arg1	counterparts					351:362	their freshwater counterparts	334:362	their freshwater counterparts	334:362	Therefore, marine bacteria must have developed adhesins with different compositions and structures than those of their freshwater counterparts to adapt to their natural environment.					
30858293	7	50	theme	physicochemical	1536:1550	arg1	properties					1552:1561	physicochemical properties	1536:1561	physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms	1536:1763	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	10	51	from	adhesion	2354:2361	arg1	environment					2377:2387	the marine environment	2366:2387	the marine environment	2366:2387	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	9	52	theme	ionic	2149:2153	arg1	strength					2155:2162	high ionic strength	2144:2162	high ionic strength	2144:2162	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	3	53	theme	clade	492:496	arg1	CaulobacteralesH					498:513	the clade CaulobacteralesH	488:513	the clade CaulobacteralesH	488:513	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	7	54	theme	marine	1498:1503	arg1	holdfasts					1521:1529	marine Caulobacterales holdfasts	1498:1529	marine Caulobacterales holdfasts	1498:1529	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	5	55	theme	similar	1026:1032	arg1	genes					1034:1038	similar genes	1026:1038	similar genes	1026:1038	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	6	56	theme	N-acetylglucosamine	1294:1312	arg1	residues					1343:1350	N-acetylglucosamine and galactose monosaccharide residues	1294:1350	N-acetylglucosamine and galactose monosaccharide residues	1294:1350	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	10	57	theme	physicochemical	2314:2328	arg1	properties					2330:2339	the physicochemical properties	2310:2339	the physicochemical properties required for adhesion in the marine environment	2310:2387	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	5	58	theme	holdfast	669:676	arg1	adhesin					678:684	The holdfast adhesin	665:684	The holdfast adhesin	665:684	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	2	59	theme	different	282:290	arg1	compositions					292:303	different compositions	282:303	different compositions	282:303	Therefore, marine bacteria must have developed adhesins with different compositions and structures than those of their freshwater counterparts to adapt to their natural environment.					
30858293	5	60	dep	compositions	820:831	arg1	use					883:885	use	883:885	use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera	883:1139	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	6	61	theme	larger	1182:1187	arg1	holdfast					1189:1196	a larger holdfast	1180:1196	a larger holdfast than C. crescentus	1180:1215	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	9	62	theme	freshwater	1961:1970	arg1	bacterium					1972:1980	the freshwater bacterium C. crescentus	1957:1994	the freshwater bacterium C. crescentus	1957:1994	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	7	63	contain	have	1531:1534	arg1	holdfasts					1521:1529	marine Caulobacterales holdfasts	1498:1529	marine Caulobacterales holdfasts	1498:1529	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	7	63	contain	have	1531:1534	arg2	properties					1552:1561	physicochemical properties	1536:1561	physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms	1536:1763	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	9	64	theme	C.	1982:1983	arg1	bacterium					1972:1980	the freshwater bacterium C. crescentus	1957:1994	the freshwater bacterium C. crescentus	1957:1994	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	6	65	theme	chemical	1255:1262	arg1	compositions					1264:1275	different chemical compositions	1245:1275	different chemical compositions	1245:1275	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	6	66	theme	H.	1160:1161	arg1	baltica					1163:1169	H. baltica	1160:1169	H. baltica	1160:1169	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	7	67	theme	higher	1431:1436	arg1	strength					1444:1451	higher ionic strength	1431:1451	higher ionic strength	1431:1451	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	10	68	theme	H.	2191:2192	arg1	model					2227:2231	an excellent model	2214:2231	an excellent model to study the effect of ionic strength on adhesion	2214:2281	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	10	68	theme	H.	2191:2192	arg1	holdfast					2202:2209	the H. baltica holdfast	2187:2209	the H. baltica holdfast	2187:2209	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	7	69	theme	life	1674:1677	arg1	part					1660:1663	a large part	1652:1663	a large part of their life spans attached to surfaces	1652:1704	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	7	70	theme	H.	1401:1402	arg1	holdfasts					1412:1420	H. baltica holdfasts	1401:1420	H. baltica holdfasts	1401:1420	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	7	71	dep	called	1749:1754	arg1	communities					1737:1747	communities	1737:1747	communities	1737:1747	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	4	72	theme	cell-cell	645:653	arg1	adhesion					655:662	cell-cell adhesion	645:662	cell-cell adhesion	645:662	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	4	73	dep	pole	580:583	arg1	stalk					611:615	the reproductive stalk	594:615	the cell pole opposite the reproductive stalk	571:615	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	9	74	theme	bacterium	2023:2031	arg1	relative					2002:2009	a relative	2000:2009	a relative	2000:2009	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	9	74	theme	bacterium	2023:2031	arg1	baltica					2036:2042	the marine bacterium H. baltica	2012:2042	the marine bacterium H. baltica	2012:2042	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	6	75	theme	galactose	1318:1326	arg1	residues					1343:1350	N-acetylglucosamine and galactose monosaccharide residues	1294:1350	N-acetylglucosamine and galactose monosaccharide residues	1294:1350	We determine that H. baltica produces a larger holdfast than C. crescentus and that the holdfasts have different chemical compositions, as they contain N-acetylglucosamine and galactose monosaccharide residues and proteins but lack DNA.					
30858293	9	76	dep	bacterium	1972:1980	arg1	crescentus					1985:1994	the freshwater bacterium C. crescentus	1957:1994	the freshwater bacterium C. crescentus	1957:1994	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	5	77	theme	holdfast	925:932	arg1	properties					934:943	holdfast properties	925:943	holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera	925:1139	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	2	78	with	adhesins	268:275	arg1	structures					309:318	structures	309:318	structures	309:318	Therefore, marine bacteria must have developed adhesins with different compositions and structures than those of their freshwater counterparts to adapt to their natural environment.					
30858293	2	78	with	adhesins	268:275	arg1	compositions					292:303	different compositions	282:303	different compositions	282:303	Therefore, marine bacteria must have developed adhesins with different compositions and structures than those of their freshwater counterparts to adapt to their natural environment.					
30858293	0	79	theme	Marine	72:77	arg1	Adhesins					95:102	Marine Caulobacterales Adhesins	72:102	Marine Caulobacterales Adhesins	72:102	Comparative Analysis of Ionic Strength Tolerance between Freshwater and Marine Caulobacterales Adhesins.					
30858293	7	80	theme	Most	1632:1635	arg1	bacteria					1637:1644	high-ionic-strength environments.IMPORTANCE Most bacteria	1588:1644	high-ionic-strength environments.IMPORTANCE Most bacteria	1588:1644	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	9	81	theme	H.	2057:2058	arg1	holdfasts					2068:2076	H. baltica holdfasts	2057:2076	H. baltica holdfasts	2057:2076	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	7	82	theme	high-ionic-strength	1588:1606	arg1	bacteria					1637:1644	high-ionic-strength environments.IMPORTANCE Most bacteria	1588:1644	high-ionic-strength environments.IMPORTANCE Most bacteria	1588:1644	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	10	83	theme	ionic	2256:2260	arg1	strength					2262:2269	ionic strength	2256:2269	ionic strength	2256:2269	Our results show that the H. baltica holdfast is an excellent model to study the effect of ionic strength on adhesion and provides insights into the physicochemical properties required for adhesion in the marine environment.					
30858293	5	84	dep	properties	934:943	arg1	show					974:977	show	974:977	show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera	974:1139	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	5	85	from	properties	837:846	arg1	Caulobacterales					858:872	marine Caulobacterales	851:872	marine Caulobacterales	851:872	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	5	86	theme	freshwater	984:993	arg1	Caulobacterales					1006:1020	freshwater and marine Caulobacterales	984:1020	Caulobacterales	1006:1020	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	3	87	theme	marine	460:465	arg1	bacterium					475:483	a marine budding bacterium	458:483	a marine budding bacterium in the clade CaulobacteralesH	458:513	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	3	87	theme	marine	460:465	arg1	baltica					447:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	5	88	theme	holdfast	811:818	arg1	compositions					820:831	holdfast compositions	811:831	holdfast compositions	811:831	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	7	89	dep	spans	1679:1683	arg1	attached					1685:1692	attached	1685:1692	spans attached to surfaces	1679:1704	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	5	90	from	properties	934:943	arg1	Caulobacterales					955:969	marine Caulobacterales	948:969	marine Caulobacterales	948:969	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	0	91	theme	Ionic	24:28	arg1	Strength					30:37	Ionic Strength	24:37	Ionic Strength	24:37	Comparative Analysis of Ionic Strength Tolerance between Freshwater and Marine Caulobacterales Adhesins.					
30858293	4	92	theme	cell	575:578	arg1	pole					580:583	the cell pole opposite the reproductive stalk	571:615	the cell pole opposite the reproductive stalk	571:615	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30858293	9	93	theme	chemical	2110:2117	arg1	composition					2119:2129	chemical composition	2110:2129	chemical composition	2110:2129	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	7	94	theme	complex	1715:1721	arg1	biofilms					1756:1763	complex multicellular communities called biofilms	1715:1763	complex multicellular communities called biofilms	1715:1763	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	3	95	theme	Hirschia	438:445	arg1	bacterium					475:483	a marine budding bacterium	458:483	a marine budding bacterium in the clade CaulobacteralesH	458:513	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	3	95	theme	Hirschia	438:445	arg1	baltica					447:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	5	96	theme	holdfast	1043:1050	arg1	biogenesis					1052:1061	holdfast biogenesis	1043:1061	holdfast biogenesis	1043:1061	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	3	97	theme	dimorphic	407:415	arg1	bacterium					475:483	a marine budding bacterium	458:483	a marine budding bacterium in the clade CaulobacteralesH	458:513	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	3	97	theme	dimorphic	407:415	arg1	baltica					447:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica	403:453	The dimorphic alphaproteobacterium Hirschia baltica is a marine budding bacterium in the clade CaulobacteralesH.					
30858293	9	98	theme	high	2144:2147	arg1	strength					2155:2162	high ionic strength	2144:2162	high ionic strength	2144:2162	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	5	99	theme	marine	999:1004	arg1	Caulobacterales					1006:1020	freshwater and marine Caulobacterales	984:1020	Caulobacterales	1006:1020	The holdfast adhesin has been best characterized in Caulobacter crescentus, a freshwater member of the Caulobacterales, and little is known about holdfast compositions and properties in marine Caulobacterales Here, we use H. baltica as a model to characterize holdfast properties in marine Caulobacterales We show that freshwater and marine Caulobacterales use similar genes in holdfast biogenesis and that these genes are highly conserved among the species in the two genera.					
30858293	7	100	theme	called	1749:1754	arg1	biofilms					1756:1763	complex multicellular communities called biofilms	1715:1763	complex multicellular communities called biofilms	1715:1763	Finally, we show that H. baltica holdfasts tolerate higher ionic strength than those of C. crescentus We conclude that marine Caulobacterales holdfasts have physicochemical properties that maximize binding in high-ionic-strength environments.IMPORTANCE Most bacteria spend a large part of their life spans attached to surfaces, forming complex multicellular communities called biofilms.					
30858293	9	101	theme	adhesive	1926:1933	arg1	holdfasts					1935:1943	the adhesive holdfasts	1922:1943	the adhesive holdfasts produced by the freshwater bacterium C. crescentus	1922:1994	In this study, we compare the adhesive holdfasts produced by the freshwater bacterium C. crescentus and a relative, the marine bacterium H. baltica We show that H. baltica holdfasts have a different morphology and chemical composition and tolerate high ionic strength.					
30858293	4	102	theme	surface	622:628	arg1	attachment					630:639	surface attachment	622:639	surface attachment	622:639	baltica uses a polar adhesin, the holdfast, located at the cell pole opposite the reproductive stalk, for surface attachment and cell-cell adhesion.					
30899062	10	0	theme	localisation	1278:1289	arg1	mechanism					1265:1273	its mechanism	1261:1273	its mechanism of localisation	1261:1289	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	1	1	theme	main	149:152	arg1	component					154:162	component	154:162	component	154:162	Bacterial cells are surrounded by cell wall, whose main component is peptidoglycan (PG), a macromolecule that withstands the internal turgor of the cell.					
30899062	0	2	theme	cell	82:85	arg1	integrity					87:95	cell integrity	82:95	cell integrity	82:95	The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity.					
30899062	8	3	theme	deleterious	950:960	arg1	effect					962:967	This deleterious effect	945:967	This deleterious effect	945:967	This deleterious effect was mitigated by drastically increasing the osmolarity of the medium, indicating that pentaglycine crosslinks are required for S. aureus cells to withstand internal turgor.					
30899062	11	4	theme	α6	1452:1453	arg1	helix					1455:1459	the α6 helix	1448:1459	the α6 helix	1448:1459	Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation.					
30899062	10	5	from	pocket	1367:1372	arg1	present					1331:1337	present	1331:1337	present	1331:1337	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	9	6	theme	canonical	1165:1173	arg1	domains					1194:1200	canonical membrane targeting domains	1165:1200	canonical membrane targeting domains	1165:1200	Despite the absence of canonical membrane targeting domains, FemA has been shown to localise at the membrane.					
30899062	11	7	theme	sharp	1470:1474	arg1	decrease					1476:1483	a sharp decrease	1468:1483	a sharp decrease in protein activity	1468:1503	Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation.					
30899062	10	8	theme	key	1318:1320	arg1	residues					1322:1329	key residues	1318:1329	key residues	1318:1329	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	11	9	theme	membrane	1553:1560	arg1	localisation					1562:1573	correct membrane localisation	1545:1573	correct membrane localisation	1545:1573	Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation.					
30899062	10	10	theme	transferase	1355:1365	arg1	pocket					1367:1372	the putative transferase pocket	1342:1372	the putative transferase pocket	1342:1372	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	7	11	theme	pseudomulticellular	863:881	arg1	forms					883:887	pseudomulticellular forms	863:887	pseudomulticellular forms that eventually lyse due to extensive membrane rupture	863:942	Depletion of femAB was lethal, with cells appearing as pseudomulticellular forms that eventually lyse due to extensive membrane rupture.					
30899062	10	12	from	mutants	1307:1313	arg1	residues					1322:1329	key residues	1318:1329	key residues	1318:1329	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	1	13	theme	cell	246:249	arg1	turgor					232:237	the internal turgor	219:237	the internal turgor of the cell	219:249	Bacterial cells are surrounded by cell wall, whose main component is peptidoglycan (PG), a macromolecule that withstands the internal turgor of the cell.					
30899062	10	14	theme	FemA	1394:1397	arg1	helix					1385:1389	the α6 helix	1378:1389	the α6 helix of FemA	1378:1397	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	10	14	theme	FemA	1394:1397	arg1	pocket					1367:1372	the putative transferase pocket	1342:1372	the putative transferase pocket	1342:1372	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	6	15	theme	FemAB	734:738	arg1	essentiality					718:729	the essentiality	714:729	the essentiality of FemAB	714:738	To investigate the essentiality of FemAB, we constructed a conditional S. aureus mutant of the femAB operon.					
30899062	2	16	theme	PG	252:253	arg1	composition					255:265	PG composition	252:265	PG composition	252:265	PG composition can vary considerably between species.					
30899062	7	17	with	lethal	831:836	arg1	cells					844:848	cells	844:848	cells appearing as pseudomulticellular forms that eventually lyse due to extensive membrane rupture	844:942	Depletion of femAB was lethal, with cells appearing as pseudomulticellular forms that eventually lyse due to extensive membrane rupture.					
30899062	10	18	theme	putative	1346:1353	arg1	pocket					1367:1372	the putative transferase pocket	1342:1372	the putative transferase pocket	1342:1372	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	11	19	from	Mutations	1435:1443	arg1	helix					1455:1459	the α6 helix	1448:1459	the α6 helix	1448:1459	Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation.					
30899062	0	20	theme	pentaglycine	4:15	arg1	bridges					17:23	The pentaglycine bridges	0:23	The pentaglycine bridges of Staphylococcus aureus peptidoglycan	0:62	The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity.					
30899062	0	20	theme	pentaglycine	4:15	arg1	essential					68:76	essential	68:76	essential	68:76	The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity.					
30899062	6	21	theme	operon	800:805	arg1	mutant					780:785	a conditional S. aureus mutant	756:785	a conditional S. aureus mutant of the femAB operon	756:805	To investigate the essentiality of FemAB, we constructed a conditional S. aureus mutant of the femAB operon.					
30899062	12	22	theme	cross	1772:1776	arg1	bridge					1778:1783	a pentaglycine cross bridge	1757:1783	a pentaglycine cross bridge	1757:1783	Our data indicates that, contrarily to what was previously thought, S. aureus cells do not survive in the absence of a pentaglycine cross bridge.					
30899062	6	23	dep	conditional	758:768	arg1	S.					770:771	S.	770:771	S.	770:771	To investigate the essentiality of FemAB, we constructed a conditional S. aureus mutant of the femAB operon.					
30899062	6	24	dep	S.	770:771	arg1	aureus					773:778	aureus	773:778	aureus	773:778	To investigate the essentiality of FemAB, we constructed a conditional S. aureus mutant of the femAB operon.					
30899062	3	25	theme	bridges	416:422	arg1	presence					398:405	the presence	394:405	the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family	394:499	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	4	26	theme	cross	537:541	arg1	bridge					543:548	the cross bridge	533:548	the cross bridge	533:548	FemX adds the first glycine of the cross bridge, while FemA and FemB add the second and the third, and the fourth and the fifth glycines, respectively.					
30899062	10	27	theme	α6	1382:1383	arg1	helix					1385:1389	the α6 helix	1378:1389	the α6 helix of FemA	1378:1397	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	1	28	theme	Bacterial	98:106	arg1	cells					108:112	Bacterial cells	98:112	Bacterial cells	98:112	Bacterial cells are surrounded by cell wall, whose main component is peptidoglycan (PG), a macromolecule that withstands the internal turgor of the cell.					
30899062	11	29	theme	FemA	1592:1595	arg1	activity					1597:1604	FemA activity	1592:1604	FemA activity	1592:1604	Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation.					
30899062	3	30	theme	crosslinked	372:382	arg1	PG					384:385	highly crosslinked PG	365:385	highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family	365:499	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	11	31	theme	correct	1545:1551	arg1	localisation					1562:1573	correct membrane localisation	1545:1573	correct membrane localisation	1545:1573	Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation.					
30899062	10	32	theme	present	1331:1337	arg1	residues					1322:1329	key residues	1318:1329	key residues	1318:1329	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	11	33	from	decrease	1476:1483	arg1	activity					1496:1503	protein activity	1488:1503	protein activity	1488:1503	Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation.					
30899062	12	34	theme	S.	1708:1709	arg1	cells					1718:1722	S. aureus cells	1708:1722	S. aureus cells	1708:1722	Our data indicates that, contrarily to what was previously thought, S. aureus cells do not survive in the absence of a pentaglycine cross bridge.					
30899062	6	35	theme	femAB	794:798	arg1	operon					800:805	the femAB operon	790:805	the femAB operon	790:805	To investigate the essentiality of FemAB, we constructed a conditional S. aureus mutant of the femAB operon.					
30899062	0	36	theme	aureus	43:48	arg1	peptidoglycan					50:62	Staphylococcus aureus peptidoglycan	28:62	Staphylococcus aureus peptidoglycan	28:62	The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity.					
30899062	4	37	theme	bridge	543:548	arg1	glycine					522:528	the first glycine	512:528	the first glycine of the cross bridge	512:548	FemX adds the first glycine of the cross bridge, while FemA and FemB add the second and the third, and the fourth and the fifth glycines, respectively.					
30899062	8	38	theme	medium	1031:1036	arg1	osmolarity					1013:1022	the osmolarity	1009:1022	the osmolarity of the medium	1009:1036	This deleterious effect was mitigated by drastically increasing the osmolarity of the medium, indicating that pentaglycine crosslinks are required for S. aureus cells to withstand internal turgor.					
30899062	9	39	theme	membrane	1175:1182	arg1	domains					1194:1200	canonical membrane targeting domains	1165:1200	canonical membrane targeting domains	1165:1200	Despite the absence of canonical membrane targeting domains, FemA has been shown to localise at the membrane.					
30899062	0	40	theme	Staphylococcus	28:41	arg1	peptidoglycan					50:62	Staphylococcus aureus peptidoglycan	28:62	Staphylococcus aureus peptidoglycan	28:62	The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity.					
30899062	4	41	theme	first	516:520	arg1	glycine					522:528	the first glycine	512:528	the first glycine of the cross bridge	512:548	FemX adds the first glycine of the cross bridge, while FemA and FemB add the second and the third, and the fourth and the fifth glycines, respectively.					
30899062	3	42	dep	pathogen	324:331	arg1	aureus					348:353	Staphylococcus aureus	333:353	The Gram-positive pathogen Staphylococcus aureus	306:353	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	9	43	theme	targeting	1184:1192	arg1	domains					1194:1200	canonical membrane targeting domains	1165:1200	canonical membrane targeting domains	1165:1200	Despite the absence of canonical membrane targeting domains, FemA has been shown to localise at the membrane.					
30899062	11	44	theme	protein	1488:1494	arg1	activity					1496:1503	protein activity	1488:1503	protein activity	1488:1503	Mutations in the α6 helix led to a sharp decrease in protein activity in vivo and in vitro but did not impair correct membrane localisation, indicating that FemA activity is not required for localisation.					
30899062	8	45	theme	internal	1125:1132	arg1	turgor					1134:1139	internal turgor	1125:1139	internal turgor	1125:1139	This deleterious effect was mitigated by drastically increasing the osmolarity of the medium, indicating that pentaglycine crosslinks are required for S. aureus cells to withstand internal turgor.					
30899062	12	46	theme	pentaglycine	1759:1770	arg1	bridge					1778:1783	a pentaglycine cross bridge	1757:1783	a pentaglycine cross bridge	1757:1783	Our data indicates that, contrarily to what was previously thought, S. aureus cells do not survive in the absence of a pentaglycine cross bridge.					
30899062	10	47	from	helix	1385:1389	arg1	present					1331:1337	present	1331:1337	present	1331:1337	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	9	48	theme	domains	1194:1200	arg1	absence					1154:1160	the absence	1150:1160	the absence of canonical membrane targeting domains	1150:1200	Despite the absence of canonical membrane targeting domains, FemA has been shown to localise at the membrane.					
30899062	3	49	theme	FemXAB	479:484	arg1	family					494:499	the FemXAB protein family	475:499	the FemXAB protein family	475:499	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	0	50	theme	peptidoglycan	50:62	arg1	bridges					17:23	The pentaglycine bridges	0:23	The pentaglycine bridges of Staphylococcus aureus peptidoglycan	0:62	The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity.					
30899062	0	50	theme	peptidoglycan	50:62	arg1	essential					68:76	essential	68:76	essential	68:76	The pentaglycine bridges of Staphylococcus aureus peptidoglycan are essential for cell integrity.					
30899062	12	51	theme	aureus	1711:1716	arg1	cells					1718:1722	S. aureus cells	1708:1722	S. aureus cells	1708:1722	Our data indicates that, contrarily to what was previously thought, S. aureus cells do not survive in the absence of a pentaglycine cross bridge.					
30899062	3	52	contain	containing	424:433	arg1	bridges					416:422	cross bridges	410:422	cross bridges	410:422	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	3	52	contain	containing	424:433	arg2	glycines					440:447	five glycines	435:447	five glycines	435:447	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	7	53	theme	membrane	927:934	arg1	rupture					936:942	extensive membrane rupture	917:942	extensive membrane rupture	917:942	Depletion of femAB was lethal, with cells appearing as pseudomulticellular forms that eventually lyse due to extensive membrane rupture.					
30899062	3	54	theme	protein	486:492	arg1	family					494:499	the FemXAB protein family	475:499	the FemXAB protein family	475:499	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	8	55	theme	pentaglycine	1055:1066	arg1	crosslinks					1068:1077	pentaglycine crosslinks	1055:1077	pentaglycine crosslinks	1055:1077	This deleterious effect was mitigated by drastically increasing the osmolarity of the medium, indicating that pentaglycine crosslinks are required for S. aureus cells to withstand internal turgor.					
30899062	3	56	link	crosslinked	372:382	arg1	PG					384:385	highly crosslinked PG	365:385	highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family	365:499	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	3	57	contain	possesses	355:363	arg1	pathogen					324:331	The Gram-positive pathogen	306:331	The Gram-positive pathogen Staphylococcus aureus	306:353	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	3	57	contain	possesses	355:363	arg2	PG					384:385	highly crosslinked PG	365:385	highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family	365:499	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	1	58	theme	cell	132:135	arg1	wall					137:140	cell wall	132:140	cell wall	132:140	Bacterial cells are surrounded by cell wall, whose main component is peptidoglycan (PG), a macromolecule that withstands the internal turgor of the cell.					
30899062	10	59	theme	tRNA	1421:1424	arg1	binding					1426:1432	tRNA binding	1421:1432	tRNA binding	1421:1432	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	6	60	theme	conditional	758:768	arg1	mutant					780:785	a conditional S. aureus mutant	756:785	a conditional S. aureus mutant of the femAB operon	756:805	To investigate the essentiality of FemAB, we constructed a conditional S. aureus mutant of the femAB operon.					
30899062	12	61	theme	bridge	1778:1783	arg1	absence					1746:1752	the absence	1742:1752	the absence of a pentaglycine cross bridge	1742:1783	Our data indicates that, contrarily to what was previously thought, S. aureus cells do not survive in the absence of a pentaglycine cross bridge.					
30899062	10	62	from	present	1331:1337	arg1	helix					1385:1389	the α6 helix	1378:1389	the α6 helix of FemA	1378:1397	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	10	62	from	present	1331:1337	arg1	pocket					1367:1372	the putative transferase pocket	1342:1372	the putative transferase pocket	1342:1372	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	7	63	theme	femAB	821:825	arg1	Depletion					808:816	Depletion	808:816	Depletion of femAB	808:825	Depletion of femAB was lethal, with cells appearing as pseudomulticellular forms that eventually lyse due to extensive membrane rupture.					
30899062	3	64	theme	Gram-positive	310:322	arg1	pathogen					324:331	The Gram-positive pathogen	306:331	The Gram-positive pathogen Staphylococcus aureus	306:353	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	4	65	dep	fourth	609:614	arg1	glycines					630:637	glycines	630:637	glycines	630:637	FemX adds the first glycine of the cross bridge, while FemA and FemB add the second and the third, and the fourth and the fifth glycines, respectively.					
30899062	7	66	theme	extensive	917:925	arg1	rupture					936:942	extensive membrane rupture	917:942	extensive membrane rupture	917:942	Depletion of femAB was lethal, with cells appearing as pseudomulticellular forms that eventually lyse due to extensive membrane rupture.					
30899062	10	67	attach	present	1331:1337	arg1	helix					1385:1389	the α6 helix	1378:1389	the α6 helix of FemA	1378:1397	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	10	67	attach	present	1331:1337	arg2	residues					1322:1329	key residues	1318:1329	key residues	1318:1329	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	10	67	attach	present	1331:1337	arg1	pocket					1367:1372	the putative transferase pocket	1342:1372	the putative transferase pocket	1342:1372	To study its mechanism of localisation, we constructed mutants in key residues present in the putative transferase pocket and the α6 helix of FemA, possibly involved in tRNA binding.					
30899062	3	68	theme	cross	410:414	arg1	bridges					416:422	cross bridges	410:422	cross bridges	410:422	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	3	69	theme	due	387:389	arg1	PG					384:385	highly crosslinked PG	365:385	highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family	365:499	The Gram-positive pathogen Staphylococcus aureus possesses highly crosslinked PG due to the presence of cross bridges containing five glycines, which are synthesised by the FemXAB protein family.					
30899062	1	70	theme	internal	223:230	arg1	turgor					232:237	the internal turgor	219:237	the internal turgor of the cell	219:249	Bacterial cells are surrounded by cell wall, whose main component is peptidoglycan (PG), a macromolecule that withstands the internal turgor of the cell.					
31246166	4	0	theme	novel	618:622	arg1	organism					624:631	the novel organism	614:631	the novel organism	614:631	The phylogenetic tree indicated that the novel organism formed a cluster with W. ghanensis LMG 24286T, W. beninensis 2L24P13T, W. fabalis M75T and W. fabaria 257T.					
31246166	11	1	theme	18423T=NBRC	1440:1450	arg1	26KH-42T					1424:1431	26KH-42T	1424:1431	26KH-42T (=KACC 18423T=NBRC 113066T)	1424:1459	The type strain is 26KH-42T (=KACC 18423T=NBRC 113066T).					
31246166	11	1	theme	18423T=NBRC	1440:1450	arg1	113066T					1452:1458	=KACC 18423T=NBRC 113066T	1434:1458	=KACC 18423T=NBRC 113066T	1434:1458	The type strain is 26KH-42T (=KACC 18423T=NBRC 113066T).					
31246166	2	2	theme	Korea	345:349	arg1	area					300:303	the mountainous area	284:303	the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea	284:349	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	3	3	theme	Weissella	468:476	arg1	%					504:504	95.9 %	499:504	95.9 %	499:504	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	3	3	theme	Weissella	468:476	arg1	2L24P13T					489:496	Weissella beninensis 2L24P13T	468:496	Weissella beninensis 2L24P13T (95.9 %)	468:505	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	7	4	dep	 0	1046:1047	arg1	C18 					1068:1071	C18 	1068:1071	C18 	1068:1071	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	4	dep	 0	1046:1047	arg1	C16 					1120:1123	C16 	1120:1123	C16 	1120:1123	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	2	5	theme	taxonomic	93:101	arg1	study					103:107	A taxonomic study	91:107	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea,	91:350	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	9	6	theme	genus	1326:1330	arg1	Weissella					1332:1340	the genus Weissella	1322:1340	the genus Weissella	1322:1340	Based on these results, strain 26KH-42T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp.					
31246166	9	7	theme	Weissellacryptocerci	1362:1381	arg1	sp					1383:1384	the name Weissellacryptocerci sp	1353:1384	the name Weissellacryptocerci sp	1353:1384	Based on these results, strain 26KH-42T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp.					
31246166	9	8	theme	Weissella	1332:1340	arg1	species					1311:1317	a novel species	1303:1317	a novel species	1303:1317	Based on these results, strain 26KH-42T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp.					
31246166	9	8	theme	Weissella	1332:1340	arg1	26KH-42T					1271:1278	strain 26KH-42T	1264:1278	strain 26KH-42T	1264:1278	Based on these results, strain 26KH-42T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp.					
31246166	0	9	theme	Weissella	0:8	arg1	sp					22:23	Weissella cryptocerci sp	0:23	Weissella cryptocerci sp.	0:24	Weissella cryptocerci sp.					
31246166	6	10	theme	unidentified	890:901	arg1	aminophospholipids					903:920	two unidentified aminophospholipids	886:920	two unidentified aminophospholipids	886:920	Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids, one unidentified phospholipid and four unidentified lipids.					
31246166	2	11	theme	rod-shaped	135:144	arg1	bacterium					196:204	a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium	112:204	bacterium	196:204	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	2	11	theme	rod-shaped	135:144	arg1	kyebangensis					256:267	Cryptocercus kyebangensis	243:267	Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea	243:349	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	9	12	theme	strain	1264:1269	arg1	species					1311:1317	a novel species	1303:1317	a novel species	1303:1317	Based on these results, strain 26KH-42T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp.					
31246166	9	12	theme	strain	1264:1269	arg1	26KH-42T					1271:1278	strain 26KH-42T	1264:1278	strain 26KH-42T	1264:1278	Based on these results, strain 26KH-42T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp.					
31246166	8	13	theme	cell-wall	1157:1165	arg1	peptidoglycan					1167:1179	The cell-wall peptidoglycan	1153:1179	The cell-wall peptidoglycan	1153:1179	The cell-wall peptidoglycan was of A4α type with the interpeptide bridge of Gly-d-Glu.					
31246166	11	14	theme	=KACC	1434:1438	arg1	26KH-42T					1424:1431	26KH-42T	1424:1431	26KH-42T (=KACC 18423T=NBRC 113066T)	1424:1459	The type strain is 26KH-42T (=KACC 18423T=NBRC 113066T).					
31246166	11	14	theme	=KACC	1434:1438	arg1	113066T					1452:1458	=KACC 18423T=NBRC 113066T	1434:1458	=KACC 18423T=NBRC 113066T	1434:1458	The type strain is 26KH-42T (=KACC 18423T=NBRC 113066T).					
31246166	1	15	theme	insect	57:62	arg1	kyebangensis					77:88	the insect Cryptocercus kyebangensis	53:88	the insect Cryptocercus kyebangensis	53:88	nov., isolated from gut of the insect Cryptocercus kyebangensis.					
31246166	2	16	attach	isolated	207:214	arg1	gut					225:227	the gut	221:227	the gut of an insect	221:240	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	2	16	attach	isolated	207:214	arg2	kyebangensis					256:267	Cryptocercus kyebangensis	243:267	Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea	243:349	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	2	16	attach	isolated	207:214	arg2	bacterium					196:204	a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium	112:204	bacterium	196:204	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	0	17	theme	cryptocerci	10:20	arg1	sp					22:23	Weissella cryptocerci sp	0:23	Weissella cryptocerci sp.	0:24	Weissella cryptocerci sp.					
31246166	2	18	theme	Gram-stain-positive	114:132	arg1	bacterium					196:204	a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium	112:204	bacterium	196:204	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	2	18	theme	Gram-stain-positive	114:132	arg1	kyebangensis					256:267	Cryptocercus kyebangensis	243:267	Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea	243:349	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	1	19	theme	Cryptocercus	64:75	arg1	kyebangensis					77:88	the insect Cryptocercus kyebangensis	53:88	the insect Cryptocercus kyebangensis	53:88	nov., isolated from gut of the insect Cryptocercus kyebangensis.					
31246166	3	20	theme	high	401:404	arg1	values					417:422	high similarity values	401:422	high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %)	401:574	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	3	21	theme	16S	371:373	arg1	rRNA					375:378	Its 16S rRNA	367:378	Its 16S rRNA gene sequence	367:392	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	8	22	with	type	1192:1195	arg1	bridge					1219:1224	the interpeptide bridge	1202:1224	the interpeptide bridge of Gly-d-Glu	1202:1237	The cell-wall peptidoglycan was of A4α type with the interpeptide bridge of Gly-d-Glu.					
31246166	1	23	theme	kyebangensis	77:88	arg1	gut					46:48	gut	46:48	gut of the insect Cryptocercus kyebangensis	46:88	nov., isolated from gut of the insect Cryptocercus kyebangensis.					
31246166	3	24	theme	similarity	406:415	arg1	values					417:422	high similarity values	401:422	high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %)	401:574	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	7	25	theme	fatty	1002:1006	arg1	C18 					1019:1022	C18 	1019:1022	C18 	1019:1022	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	25	theme	fatty	1002:1006	arg1	acids					1008:1012	The major cellular fatty acids	983:1012	The major cellular fatty acids	983:1012	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	5	26	theme	whole-genome	791:802	arg1	sequence					804:811	the whole-genome sequence	787:811	the whole-genome sequence	787:811	The G+C content was 41.1 mol% on the basis of the whole-genome sequence.					
31246166	8	27	theme	A4α	1188:1190	arg1	type					1192:1195	A4α type	1188:1195	A4α type	1188:1195	The cell-wall peptidoglycan was of A4α type with the interpeptide bridge of Gly-d-Glu.					
31246166	3	28	theme	LMG	447:449	arg1	%					464:464	95.9 %	459:464	95.9 %	459:464	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	3	28	theme	LMG	447:449	arg1	24286T					451:456	Weissella ghanensis LMG 24286T	427:456	Weissella ghanensis LMG 24286T (95.9 %)	427:465	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	3	29	dep	Weissella	468:476	arg1	beninensis					478:487	beninensis	478:487	beninensis	478:487	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	2	30	theme	mountainous	288:298	arg1	area					300:303	the mountainous area	284:303	the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea	284:349	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	3	31	dep	Weissella	544:552	arg1	fabaria					554:560	fabaria	554:560	fabaria	554:560	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	2	32	dep	rod-shaped	135:144	arg1	non-spore-forming					159:175	non-spore-forming	159:175	non-spore-forming	159:175	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	2	32	dep	rod-shaped	135:144	arg1	non-motile					147:156	non-motile	147:156	non-motile	147:156	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	7	33	theme	major	987:991	arg1	C18 					1019:1022	C18 	1019:1022	C18 	1019:1022	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	33	theme	major	987:991	arg1	acids					1008:1012	The major cellular fatty acids	983:1012	The major cellular fatty acids	983:1012	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	4	34	theme	phylogenetic	581:592	arg1	tree					594:597	The phylogenetic tree	577:597	The phylogenetic tree	577:597	The phylogenetic tree indicated that the novel organism formed a cluster with W. ghanensis LMG 24286T, W. beninensis 2L24P13T, W. fabalis M75T and W. fabaria 257T.					
31246166	3	35	theme	Weissella	427:435	arg1	%					464:464	95.9 %	459:464	95.9 %	459:464	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	3	35	theme	Weissella	427:435	arg1	24286T					451:456	Weissella ghanensis LMG 24286T	427:456	Weissella ghanensis LMG 24286T (95.9 %)	427:465	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	7	36	dep	C18 	1019:1022	arg1	C14 					1041:1044	C14 	1041:1044	C14 	1041:1044	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	36	dep	C18 	1019:1022	arg1	C16 					1032:1035	C16 	1032:1035	C16 	1032:1035	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	36	dep	C18 	1019:1022	arg1	feature					1109:1115	summed feature 8	1102:1117	summed feature 8	1102:1117	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	36	dep	C18 	1019:1022	arg1	 0					1046:1047	 0	1046:1047	 0	1046:1047	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	36	dep	C18 	1019:1022	arg1	feature					1057:1063	summed feature 8	1050:1065	summed feature 8	1050:1065	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	37	theme	cellular	993:1000	arg1	C18 					1019:1022	C18 	1019:1022	C18 	1019:1022	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	37	theme	cellular	993:1000	arg1	acids					1008:1012	The major cellular fatty acids	983:1012	The major cellular fatty acids	983:1012	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	6	38	theme	unidentified	962:973	arg1	lipids					975:980	four unidentified lipids	957:980	four unidentified lipids	957:980	Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids, one unidentified phospholipid and four unidentified lipids.					
31246166	3	39	theme	rRNA	375:378	arg1	sequence					385:392	Its 16S rRNA gene sequence	367:392	Its 16S rRNA gene sequence	367:392	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	8	40	theme	interpeptide	1206:1217	arg1	bridge					1219:1224	the interpeptide bridge	1202:1224	the interpeptide bridge of Gly-d-Glu	1202:1237	The cell-wall peptidoglycan was of A4α type with the interpeptide bridge of Gly-d-Glu.					
31246166	2	41	theme	Republic	333:340	arg1	area					300:303	the mountainous area	284:303	the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea	284:349	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	3	42	theme	gene	380:383	arg1	sequence					385:392	Its 16S rRNA gene sequence	367:392	Its 16S rRNA gene sequence	367:392	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	6	43	theme	Polar	814:818	arg1	lipids					820:825	Polar lipids	814:825	Polar lipids	814:825	Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids, one unidentified phospholipid and four unidentified lipids.					
31246166	5	44	theme	sequence	804:811	arg1	basis					778:782	the basis	774:782	the basis of the whole-genome sequence	774:811	The G+C content was 41.1 mol% on the basis of the whole-genome sequence.					
31246166	8	45	theme	Gly-d-Glu	1229:1237	arg1	bridge					1219:1224	the interpeptide bridge	1202:1224	the interpeptide bridge of Gly-d-Glu	1202:1237	The cell-wall peptidoglycan was of A4α type with the interpeptide bridge of Gly-d-Glu.					
31246166	7	46	dep	C18 	1068:1071	arg1	 1ω6c					1091:1095	 1ω6c	1091:1095	 1ω6c	1091:1095	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	46	dep	C18 	1068:1071	arg1	C18 					1086:1089	C18 	1086:1089	C18 	1086:1089	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	46	dep	C18 	1068:1071	arg1	 1ω7c					1073:1077	 1ω7c	1073:1077	 1ω7c	1073:1077	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	3	47	dep	Weissella	427:435	arg1	ghanensis					437:445	ghanensis	437:445	ghanensis	437:445	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	2	48	theme	Cryptocercus	243:254	arg1	bacterium					196:204	a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium	112:204	bacterium	196:204	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	2	48	theme	Cryptocercus	243:254	arg1	kyebangensis					256:267	Cryptocercus kyebangensis	243:267	Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea	243:349	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	9	49	theme	name	1357:1360	arg1	sp					1383:1384	the name Weissellacryptocerci sp	1353:1384	the name Weissellacryptocerci sp	1353:1384	Based on these results, strain 26KH-42T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp.					
31246166	7	50	dep	C16 	1120:1123	arg1	ω7c					1128:1130	ω7c	1128:1130	ω7c	1128:1130	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	50	dep	C16 	1120:1123	arg1	C16 					1139:1142	C16 	1139:1142	C16 	1139:1142	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	50	dep	C16 	1120:1123	arg1	ω6c					1147:1149	ω6c	1147:1149	ω6c	1147:1149	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	2	51	theme	bacterium	196:204	arg1	study					103:107	A taxonomic study	91:107	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea,	91:350	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	5	52	from	%	769:769	arg1	basis					778:782	the basis	774:782	the basis of the whole-genome sequence	774:811	The G+C content was 41.1 mol% on the basis of the whole-genome sequence.					
31246166	7	53	theme	summed	1102:1107	arg1	feature					1109:1115	summed feature 8	1102:1117	summed feature 8	1102:1117	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	2	54	theme	catalase-negative	178:194	arg1	bacterium					196:204	a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium	112:204	bacterium	196:204	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	2	54	theme	catalase-negative	178:194	arg1	kyebangensis					256:267	Cryptocercus kyebangensis	243:267	Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea	243:349	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	2	55	theme	insect	235:240	arg1	gut					225:227	the gut	221:227	the gut of an insect	221:240	A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted.					
31246166	11	56	theme	type	1409:1412	arg1	26KH-42T					1424:1431	26KH-42T	1424:1431	26KH-42T (=KACC 18423T=NBRC 113066T)	1424:1459	The type strain is 26KH-42T (=KACC 18423T=NBRC 113066T).					
31246166	11	56	theme	type	1409:1412	arg1	strain					1414:1419	The type strain	1405:1419	The type strain	1405:1419	The type strain is 26KH-42T (=KACC 18423T=NBRC 113066T).					
31246166	9	57	theme	novel	1305:1309	arg1	species					1311:1317	a novel species	1303:1317	a novel species	1303:1317	Based on these results, strain 26KH-42T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp.					
31246166	9	57	theme	novel	1305:1309	arg1	26KH-42T					1271:1278	strain 26KH-42T	1264:1278	strain 26KH-42T	1264:1278	Based on these results, strain 26KH-42T could be classified as a novel species of the genus Weissella, for which the name Weissellacryptocerci sp.					
31246166	5	58	theme	41.1 mol	761:768	arg1	G+C content					745:755	The G+C content	741:755	The G+C content	741:755	The G+C content was 41.1 mol% on the basis of the whole-genome sequence.					
31246166	5	58	theme	41.1 mol	761:768	arg1	%					769:769	41.1 mol%	761:769	41.1 mol%	761:769	The G+C content was 41.1 mol% on the basis of the whole-genome sequence.					
31246166	4	59	with	cluster	642:648	arg1	257T					735:738	257T	735:738	257T	735:738	The phylogenetic tree indicated that the novel organism formed a cluster with W. ghanensis LMG 24286T, W. beninensis 2L24P13T, W. fabalis M75T and W. fabaria 257T.					
31246166	4	59	with	cluster	642:648	arg1	M75T					715:718	M75T	715:718	M75T	715:718	The phylogenetic tree indicated that the novel organism formed a cluster with W. ghanensis LMG 24286T, W. beninensis 2L24P13T, W. fabalis M75T and W. fabaria 257T.					
31246166	4	59	with	cluster	642:648	arg1	24286T					672:677	24286T	672:677	24286T	672:677	The phylogenetic tree indicated that the novel organism formed a cluster with W. ghanensis LMG 24286T, W. beninensis 2L24P13T, W. fabalis M75T and W. fabaria 257T.					
31246166	4	59	with	cluster	642:648	arg1	beninensis					683:692	beninensis	683:692	beninensis	683:692	The phylogenetic tree indicated that the novel organism formed a cluster with W. ghanensis LMG 24286T, W. beninensis 2L24P13T, W. fabalis M75T and W. fabaria 257T.					
31246166	3	60	theme	Weissella	544:552	arg1	%					573:573	95.7 %	568:573	95.7 %	568:573	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	3	60	theme	Weissella	544:552	arg1	257T					562:565	Weissella fabaria 257T	544:565	Weissella fabaria 257T (95.7 %)	544:574	Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286T (95.9 %), Weissella beninensis 2L24P13T (95.9 %), Weissella fabalis M75T (95.7 %) and Weissella fabaria 257T (95.7 %).					
31246166	7	61	theme	summed	1050:1055	arg1	 0					1046:1047	 0	1046:1047	 0	1046:1047	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	7	61	theme	summed	1050:1055	arg1	feature					1057:1063	summed feature 8	1050:1065	summed feature 8	1050:1065	The major cellular fatty acids were C18 : 1 ω9c, C16 : 0, C14 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 8 (C16 : 1 ω7c and/or C16 : 1 ω6c).					
31246166	6	62	theme	unidentified	927:938	arg1	phospholipid					940:951	one unidentified phospholipid	923:951	one unidentified phospholipid	923:951	Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids, one unidentified phospholipid and four unidentified lipids.					
30690690	4	0	theme	mountain	871:878	arg1	extract					885:891	Sideritis scardica (mountain tea) extract	851:891	Sideritis scardica (mountain tea) extract	851:891	The aim of this study was to investigate the influence of Sideritis scardica (mountain tea) extract on metabolic disturbances induced by ovariectomy in rats.					
30690690	3	1	dep	alternative	665:675	arg1	a					654:654	a	654:654	a	654:654	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	7	2	theme	Ovariectomized	1432:1445	arg1	rats					1447:1450	Ovariectomized rats	1432:1450	Ovariectomized rats treated with S. scardica extract	1432:1483	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	2	3	theme	replacement	287:297	arg1	therapy					299:305	Estrogen replacement therapy	278:305	Estrogen replacement therapy	278:305	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	6	4	theme	glucose	1210:1216	arg1	levels					1218:1223	fasting glucose levels	1202:1223	fasting glucose levels	1202:1223	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	1	5	theme	metabolic	171:179	arg1	disturbances					181:192	deep metabolic disturbances	166:192	deep metabolic disturbances	166:192	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	1	5	theme	metabolic	171:179	arg1	adiposity					236:244	adiposity	236:244	adiposity	236:244	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	1	5	theme	metabolic	171:179	arg1	changes					251:257	changes	251:257	changes in lipid profiles	251:275	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	1	5	theme	metabolic	171:179	arg1	sensitivity					223:233	decreased insulin sensitivity	205:233	decreased insulin sensitivity	205:233	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	4	6	theme	scardica	861:868	arg1	extract					885:891	Sideritis scardica (mountain tea) extract	851:891	Sideritis scardica (mountain tea) extract	851:891	The aim of this study was to investigate the influence of Sideritis scardica (mountain tea) extract on metabolic disturbances induced by ovariectomy in rats.					
30690690	6	7	from	content	1277:1283	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	8	8	theme	AMP-activated	1888:1900	arg1	kinase					1910:1915	AMP-activated protein kinase	1888:1915	AMP-activated protein kinase	1888:1915	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy was associated with the activation of AMP-activated protein kinase in liver cells.					
30690690	2	9	theme	thromboembolic	540:553	arg1	disease					555:561	cardiovascular and thromboembolic disease	521:561	disease	555:561	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	2	10	from	safe	360:363	arg1	women					396:400	most healthy postmenopausal women	368:400	most healthy postmenopausal women	368:400	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	8	11	theme	kinase	1910:1915	arg1	activation					1874:1883	the activation	1870:1883	the activation of AMP-activated protein kinase in liver cells	1870:1930	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy was associated with the activation of AMP-activated protein kinase in liver cells.					
30690690	7	12	contain	had	1485:1487	arg2	triglycerides					1501:1513	lower blood triglycerides	1489:1513	lower blood triglycerides	1489:1513	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	7	12	contain	had	1485:1487	arg2	concentration					1699:1711	thiol group concentration	1687:1711	thiol group concentration	1687:1711	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	7	12	contain	had	1485:1487	arg1	rats					1447:1450	Ovariectomized rats	1432:1450	Ovariectomized rats treated with S. scardica extract	1432:1483	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	7	12	contain	had	1485:1487	arg2	activity					1674:1681	significantly higher catalase activity	1644:1681	significantly higher catalase activity	1644:1681	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	7	13	theme	glucose	1587:1593	arg1	challenge					1595:1603	oral glucose challenge	1582:1603	oral glucose challenge	1582:1603	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	2	14	theme	cardiovascular	521:534	arg1	disease					555:561	cardiovascular and thromboembolic disease	521:561	disease	555:561	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	0	15	from	Levels	73:78	arg1	Rats					130:133	Ovariectomized Rats	115:133	Ovariectomized Rats	115:133	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	6	16	theme	glycogen	1268:1275	arg1	content					1277:1283	liver glycogen content	1262:1283	liver glycogen content	1262:1283	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	6	17	theme	kinase	1387:1392	arg1	activity					1394:1401	AMP-activated protein kinase activity	1365:1401	AMP-activated protein kinase activity	1365:1401	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	5	18	theme	200 mg/kg	1038:1046	arg1	extract					1060:1066	200 mg/kg S. scardica extract	1038:1066	200 mg/kg S. scardica extract	1038:1066	The study included 24 rats divided into three groups: ovariectomized rats treated with 200 mg/kg S. scardica extract for 24 weeks (n = 8), ovariectomized non-treated (n = 8), and Sham-operated (n = 8) rats.					
30690690	3	19	theme	natural	583:589	arg1	compounds					591:599	certain natural compounds	575:599	certain natural compounds with positive metabolic effects	575:631	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	3	19	theme	natural	583:589	arg1	alternative					665:675	possible alternative	656:675	possible alternative	656:675	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	3	19	theme	natural	583:589	arg1	treatment					691:699	adjunctive treatment	680:699	adjunctive treatment	680:699	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	6	20	theme	weight	1171:1176	arg1	gain					1178:1181	weight gain	1171:1181	weight gain	1171:1181	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	6	21	from	gain	1178:1181	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	6	22	theme	glucose	1243:1249	arg1	challenge					1251:1259	oral glucose challenge	1238:1259	oral glucose challenge	1238:1259	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	5	23	theme	scardica	1051:1058	arg1	extract					1060:1066	200 mg/kg S. scardica extract	1038:1066	200 mg/kg S. scardica extract	1038:1066	The study included 24 rats divided into three groups: ovariectomized rats treated with 200 mg/kg S. scardica extract for 24 weeks (n = 8), ovariectomized non-treated (n = 8), and Sham-operated (n = 8) rats.					
30690690	7	24	theme	group	1693:1697	arg1	concentration					1699:1711	thiol group concentration	1687:1711	thiol group concentration	1687:1711	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	0	25	from	Markers	84:90	arg1	Rats					130:133	Ovariectomized Rats	115:133	Ovariectomized Rats	115:133	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	6	26	theme	malondialdehyde	1323:1337	arg1	concentrations					1339:1352	malondialdehyde concentrations	1323:1352	malondialdehyde concentrations	1323:1352	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	6	27	theme	protein	1379:1385	arg1	kinase					1387:1392	AMP-activated protein kinase	1365:1392	AMP-activated protein kinase activity	1365:1401	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	8	28	theme	scardica	1769:1776	arg1	extract					1778:1784	S. scardica extract	1766:1784	S. scardica extract	1766:1784	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy was associated with the activation of AMP-activated protein kinase in liver cells.					
30690690	2	29	theme	endometrial	480:490	arg1	cancer					492:497	endometrial cancer	480:497	endometrial cancer	480:497	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	1	30	from	adiposity	236:244	arg1	profiles					268:275	lipid profiles	262:275	lipid profiles	262:275	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	6	31	from	concentrations	1339:1352	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	7	32	theme	catalase	1665:1672	arg1	activity					1674:1681	significantly higher catalase activity	1644:1681	significantly higher catalase activity	1644:1681	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	7	33	theme	glucose	1532:1538	arg1	levels					1540:1545	fasting glucose levels	1524:1545	fasting glucose levels	1524:1545	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	0	34	from	Effects	0:6	arg1	Levels					73:78	Triglyceride Levels	60:78	Triglyceride Levels	60:78	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	0	34	from	Effects	0:6	arg1	Markers					84:90	Markers	84:90	Markers of Oxidative Stress in Ovariectomized Rats	84:133	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	0	34	from	Effects	0:6	arg1	Tolerance					49:57	Glucose Tolerance	41:57	Glucose Tolerance	41:57	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	6	35	theme	thiol	1305:1309	arg1	groups					1311:1316	thiol groups	1305:1316	thiol groups	1305:1316	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	8	36	theme	metabolic	1799:1807	arg1	disturbances					1809:1820	metabolic disturbances	1799:1820	metabolic disturbances associated with ovariectomy	1799:1848	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy was associated with the activation of AMP-activated protein kinase in liver cells.					
30690690	0	37	theme	Oxidative	95:103	arg1	Stress					105:110	Oxidative Stress	95:110	Oxidative Stress	95:110	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	6	38	from	groups	1311:1316	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	2	39	dep	existing	419:426	arg1	concerns					428:435	concerns	428:435	are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease	409:561	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	2	40	theme	healthy	373:379	arg1	women					396:400	most healthy postmenopausal women	368:400	most healthy postmenopausal women	368:400	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	5	41	theme	n = 8	1145:1149	arg1	weeks					1075:1079	24 weeks	1072:1079	24 weeks (n = 8)	1072:1087	The study included 24 rats divided into three groups: ovariectomized rats treated with 200 mg/kg S. scardica extract for 24 weeks (n = 8), ovariectomized non-treated (n = 8), and Sham-operated (n = 8) rats.					
30690690	5	41	theme	n = 8	1145:1149	arg1	rats					1152:1155	Sham-operated (n = 8) rats	1130:1155	Sham-operated (n = 8) rats	1130:1155	The study included 24 rats divided into three groups: ovariectomized rats treated with 200 mg/kg S. scardica extract for 24 weeks (n = 8), ovariectomized non-treated (n = 8), and Sham-operated (n = 8) rats.					
30690690	7	42	theme	glycogen	1622:1629	arg1	content					1631:1637	liver glycogen content	1616:1637	liver glycogen content	1616:1637	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	0	43	theme	Sideritis	11:19	arg1	Extract					30:36	Sideritis scardica Extract	11:36	Sideritis scardica Extract	11:36	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	3	44	dep	patients	704:711	arg1	willing					717:723	willing	717:723	not willing to take estrogens or patients with contraindications for estrogens	713:790	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	7	45	theme	blood	1495:1499	arg1	triglycerides					1501:1513	lower blood triglycerides	1489:1513	lower blood triglycerides	1489:1513	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	0	46	theme	Extract	30:36	arg1	Effects					0:6	Effects	0:6	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.	0:134	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	1	47	theme	lipid	262:266	arg1	profiles					268:275	lipid profiles	262:275	lipid profiles	262:275	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	0	48	theme	Glucose	41:47	arg1	Tolerance					49:57	Glucose Tolerance	41:57	Glucose Tolerance	41:57	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	5	49	theme	Sham-operated	1130:1142	arg1	weeks					1075:1079	24 weeks	1072:1079	24 weeks (n = 8)	1072:1087	The study included 24 rats divided into three groups: ovariectomized rats treated with 200 mg/kg S. scardica extract for 24 weeks (n = 8), ovariectomized non-treated (n = 8), and Sham-operated (n = 8) rats.					
30690690	5	49	theme	Sham-operated	1130:1142	arg1	rats					1152:1155	Sham-operated (n = 8) rats	1130:1155	Sham-operated (n = 8) rats	1130:1155	The study included 24 rats divided into three groups: ovariectomized rats treated with 200 mg/kg S. scardica extract for 24 weeks (n = 8), ovariectomized non-treated (n = 8), and Sham-operated (n = 8) rats.					
30690690	7	50	theme	scardica	1468:1475	arg1	extract					1477:1483	S. scardica extract	1465:1483	S. scardica extract	1465:1483	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	2	51	from	women	396:400	arg1	safe					360:363	safe	360:363	safe	360:363	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	3	52	theme	metabolic	615:623	arg1	effects					625:631	positive metabolic effects	606:631	positive metabolic effects	606:631	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	3	53	theme	adjunctive	680:689	arg1	compounds					591:599	certain natural compounds	575:599	certain natural compounds with positive metabolic effects	575:631	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	3	53	theme	adjunctive	680:689	arg1	treatment					691:699	adjunctive treatment	680:699	adjunctive treatment	680:699	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	6	54	from	response	1226:1233	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	4	55	theme	study	809:813	arg1	aim					797:799	The aim	793:799	The aim of this study	793:813	The aim of this study was to investigate the influence of Sideritis scardica (mountain tea) extract on metabolic disturbances induced by ovariectomy in rats.					
30690690	3	56	with	estrogens	733:741	arg1	contraindications					760:776	contraindications	760:776	contraindications for estrogens	760:790	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	3	57	with	compounds	591:599	arg1	effects					625:631	positive metabolic effects	606:631	positive metabolic effects	606:631	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	6	58	theme	oral	1238:1241	arg1	challenge					1251:1259	oral glucose challenge	1238:1259	oral glucose challenge	1238:1259	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	8	59	theme	protein	1902:1908	arg1	kinase					1910:1915	AMP-activated protein kinase	1888:1915	AMP-activated protein kinase	1888:1915	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy was associated with the activation of AMP-activated protein kinase in liver cells.					
30690690	6	60	theme	liver	1406:1410	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	6	61	from	levels	1218:1223	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	4	62	theme	tea	880:882	arg1	extract					885:891	Sideritis scardica (mountain tea) extract	851:891	Sideritis scardica (mountain tea) extract	851:891	The aim of this study was to investigate the influence of Sideritis scardica (mountain tea) extract on metabolic disturbances induced by ovariectomy in rats.					
30690690	2	63	theme	Estrogen	278:285	arg1	therapy					299:305	Estrogen replacement therapy	278:305	Estrogen replacement therapy	278:305	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	1	64	theme	deep	166:169	arg1	disturbances					181:192	deep metabolic disturbances	166:192	deep metabolic disturbances	166:192	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	1	64	theme	deep	166:169	arg1	adiposity					236:244	adiposity	236:244	adiposity	236:244	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	1	64	theme	deep	166:169	arg1	changes					251:257	changes	251:257	changes in lipid profiles	251:275	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	1	64	theme	deep	166:169	arg1	sensitivity					223:233	decreased insulin sensitivity	205:233	decreased insulin sensitivity	205:233	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	6	65	theme	fasting	1202:1208	arg1	levels					1218:1223	fasting glucose levels	1202:1223	fasting glucose levels	1202:1223	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	4	66	theme	Sideritis	851:859	arg1	extract					885:891	Sideritis scardica (mountain tea) extract	851:891	Sideritis scardica (mountain tea) extract	851:891	The aim of this study was to investigate the influence of Sideritis scardica (mountain tea) extract on metabolic disturbances induced by ovariectomy in rats.					
30690690	7	67	theme	ovariectomized	1730:1743	arg1	rats					1745:1748	non-treated ovariectomized rats	1718:1748	non-treated ovariectomized rats	1718:1748	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	6	68	theme	catalase	1286:1293	arg1	activity					1295:1302	catalase activity	1286:1302	catalase activity	1286:1302	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	7	69	theme	oral	1582:1585	arg1	challenge					1595:1603	oral glucose challenge	1582:1603	oral glucose challenge	1582:1603	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	3	70	from	treatment	691:699	arg1	patients					704:711	patients	704:711	patients not willing to take estrogens or patients with contraindications for estrogens	704:790	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	1	71	from	changes	251:257	arg1	profiles					268:275	lipid profiles	262:275	lipid profiles	262:275	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	1	72	theme	insulin	215:221	arg1	sensitivity					223:233	decreased insulin sensitivity	205:233	decreased insulin sensitivity	205:233	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	6	73	theme	body	1184:1187	arg1	composition					1189:1199	body composition	1184:1199	body composition	1184:1199	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	8	74	from	activation	1874:1883	arg1	cells					1926:1930	liver cells	1920:1930	liver cells	1920:1930	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy was associated with the activation of AMP-activated protein kinase in liver cells.					
30690690	6	75	theme	liver	1262:1266	arg1	content					1277:1283	liver glycogen content	1262:1283	liver glycogen content	1262:1283	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	6	76	from	activity	1394:1401	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	3	77	theme	certain	575:581	arg1	compounds					591:599	certain natural compounds	575:599	certain natural compounds with positive metabolic effects	575:631	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	3	77	theme	certain	575:581	arg1	alternative					665:675	possible alternative	656:675	possible alternative	656:675	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	3	77	theme	certain	575:581	arg1	treatment					691:699	adjunctive treatment	680:699	adjunctive treatment	680:699	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	1	78	from	sensitivity	223:233	arg1	profiles					268:275	lipid profiles	262:275	lipid profiles	262:275	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	0	79	theme	Ovariectomized	115:128	arg1	Rats					130:133	Ovariectomized Rats	115:133	Ovariectomized Rats	115:133	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	7	80	theme	lower	1556:1560	arg1	glucose					1562:1568	well lower glucose	1551:1568	well lower glucose	1551:1568	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	4	81	theme	extract	885:891	arg1	influence					838:846	the influence	834:846	the influence of Sideritis scardica (mountain tea) extract on metabolic disturbances induced by ovariectomy in rats	834:948	The aim of this study was to investigate the influence of Sideritis scardica (mountain tea) extract on metabolic disturbances induced by ovariectomy in rats.					
30690690	5	82	theme	S.	1048:1049	arg1	extract					1060:1066	200 mg/kg S. scardica extract	1038:1066	200 mg/kg S. scardica extract	1038:1066	The study included 24 rats divided into three groups: ovariectomized rats treated with 200 mg/kg S. scardica extract for 24 weeks (n = 8), ovariectomized non-treated (n = 8), and Sham-operated (n = 8) rats.					
30690690	7	83	theme	non-treated	1718:1728	arg1	rats					1745:1748	non-treated ovariectomized rats	1718:1748	non-treated ovariectomized rats	1718:1748	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	4	84	theme	metabolic	896:904	arg1	disturbances					906:917	metabolic disturbances	896:917	metabolic disturbances induced by ovariectomy in rats	896:948	The aim of this study was to investigate the influence of Sideritis scardica (mountain tea) extract on metabolic disturbances induced by ovariectomy in rats.					
30690690	6	85	from	composition	1189:1199	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	0	86	theme	Triglyceride	60:71	arg1	Levels					73:78	Triglyceride Levels	60:78	Triglyceride Levels	60:78	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	3	87	theme	positive	606:613	arg1	effects					625:631	positive metabolic effects	606:631	positive metabolic effects	606:631	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	6	88	theme	Food	1158:1161	arg1	intake					1163:1168	Food intake	1158:1168	Food intake	1158:1168	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	7	89	dep	had	1485:1487	arg1	increased					1606:1614	increased	1606:1614	increased liver glycogen content	1606:1637	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	7	89	dep	had	1485:1487	arg1	reduced					1516:1522	reduced	1516:1522	reduced	1516:1522	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	6	90	from	intake	1163:1168	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	8	91	theme	S.	1766:1767	arg1	extract					1778:1784	S. scardica extract	1766:1784	S. scardica extract	1766:1784	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy was associated with the activation of AMP-activated protein kinase in liver cells.					
30690690	7	92	theme	thiol	1687:1691	arg1	concentration					1699:1711	thiol group concentration	1687:1711	thiol group concentration	1687:1711	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	7	93	theme	fasting	1524:1530	arg1	levels					1540:1545	fasting glucose levels	1524:1545	fasting glucose levels	1524:1545	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	8	94	theme	extract	1778:1784	arg1	ability					1755:1761	The ability	1751:1761	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy	1751:1848	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy was associated with the activation of AMP-activated protein kinase in liver cells.					
30690690	0	95	from	Tolerance	49:57	arg1	Rats					130:133	Ovariectomized Rats	115:133	Ovariectomized Rats	115:133	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	8	96	theme	liver	1920:1924	arg1	cells					1926:1930	liver cells	1920:1930	liver cells	1920:1930	The ability of S. scardica extract to attenuate metabolic disturbances associated with ovariectomy was associated with the activation of AMP-activated protein kinase in liver cells.					
30690690	0	97	theme	Stress	105:110	arg1	Levels					73:78	Triglyceride Levels	60:78	Triglyceride Levels	60:78	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	0	97	theme	Stress	105:110	arg1	Markers					84:90	Markers	84:90	Markers of Oxidative Stress in Ovariectomized Rats	84:133	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	0	97	theme	Stress	105:110	arg1	Tolerance					49:57	Glucose Tolerance	41:57	Glucose Tolerance	41:57	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	6	98	from	activity	1295:1302	arg1	cells					1412:1416	liver cells	1406:1416	liver cells	1406:1416	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	1	99	theme	decreased	205:213	arg1	sensitivity					223:233	decreased insulin sensitivity	205:233	decreased insulin sensitivity	205:233	Menopause is characterized by deep metabolic disturbances, including decreased insulin sensitivity, adiposity, and changes in lipid profiles.					
30690690	7	100	theme	higher	1658:1663	arg1	activity					1674:1681	significantly higher catalase activity	1644:1681	significantly higher catalase activity	1644:1681	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	2	101	theme	postmenopausal	381:394	arg1	women					396:400	most healthy postmenopausal women	368:400	most healthy postmenopausal women	368:400	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	4	102	from	influence	838:846	arg1	disturbances					906:917	metabolic disturbances	896:917	metabolic disturbances induced by ovariectomy in rats	896:948	The aim of this study was to investigate the influence of Sideritis scardica (mountain tea) extract on metabolic disturbances induced by ovariectomy in rats.					
30690690	3	103	with	patients	746:753	arg1	contraindications					760:776	contraindications	760:776	contraindications for estrogens	760:790	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	2	104	theme	most	368:371	arg1	women					396:400	most healthy postmenopausal women	368:400	most healthy postmenopausal women	368:400	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	0	105	theme	scardica	21:28	arg1	Extract					30:36	Sideritis scardica Extract	11:36	Sideritis scardica Extract	11:36	Effects of Sideritis scardica Extract on Glucose Tolerance, Triglyceride Levels and Markers of Oxidative Stress in Ovariectomized Rats.					
30690690	6	106	theme	AMP-activated	1365:1377	arg1	kinase					1387:1392	AMP-activated protein kinase	1365:1392	AMP-activated protein kinase activity	1365:1401	Food intake, weight gain, body composition, fasting glucose levels, response to oral glucose challenge, liver glycogen content, catalase activity, thiol groups, and malondialdehyde concentrations as well as AMP-activated protein kinase activity in liver cells were studied.					
30690690	5	107	theme	ovariectomized	1005:1018	arg1	rats					1020:1023	ovariectomized rats	1005:1023	ovariectomized rats	1005:1023	The study included 24 rats divided into three groups: ovariectomized rats treated with 200 mg/kg S. scardica extract for 24 weeks (n = 8), ovariectomized non-treated (n = 8), and Sham-operated (n = 8) rats.					
30690690	7	108	theme	liver	1616:1620	arg1	content					1631:1637	liver glycogen content	1616:1637	liver glycogen content	1616:1637	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	7	109	theme	lower	1489:1493	arg1	triglycerides					1501:1513	lower blood triglycerides	1489:1513	lower blood triglycerides	1489:1513	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	2	110	theme	increased	450:458	arg1	risk					460:463	an increased risk	447:463	an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease	447:561	Estrogen replacement therapy can partially reverse these changes, and while it is safe in most healthy postmenopausal women, there are still existing concerns regarding an increased risk for breast and endometrial cancer as well as a risk for cardiovascular and thromboembolic disease.					
30690690	3	111	theme	possible	656:663	arg1	compounds					591:599	certain natural compounds	575:599	certain natural compounds with positive metabolic effects	575:631	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	3	111	theme	possible	656:663	arg1	alternative					665:675	possible alternative	656:675	possible alternative	656:675	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30690690	7	112	theme	S.	1465:1466	arg1	extract					1477:1483	S. scardica extract	1465:1483	S. scardica extract	1465:1483	Ovariectomized rats treated with S. scardica extract had lower blood triglycerides, reduced fasting glucose levels, as well lower glucose peaks after oral glucose challenge, increased liver glycogen content, and significantly higher catalase activity and thiol group concentration than non-treated ovariectomized rats.					
30690690	3	113	from	alternative	665:675	arg1	patients					704:711	patients	704:711	patients not willing to take estrogens or patients with contraindications for estrogens	704:790	Therefore, certain natural compounds with positive metabolic effects may be considered as a possible alternative or adjunctive treatment in patients not willing to take estrogens or patients with contraindications for estrogens.					
30197135	7	0	theme	protein	968:974	arg1	g/kg					981:984	total protein 24.2 g/kg	962:984	total protein 24.2 g/kg (made up of casein, 14.6 g/kg, and whey protein, 9.5 g/kg)	962:1043	The basic composition of the milk was as follows: solids-not-fat, 84.4 g/kg, fat 15.1 g/kg, lactose 65.3 g/kg, and total protein 24.2 g/kg (made up of casein, 14.6 g/kg, and whey protein, 9.5 g/kg).					
30197135	2	1	theme	given	292:296	arg1	breed					304:308	a given horse breed	290:308	a given horse breed	290:308	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	3	2	theme	mare	499:502	arg1	milk					504:507	Polish Coldblood mare milk	482:507	Polish Coldblood mare milk	482:507	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30197135	3	3	theme	Coldblood	489:497	arg1	milk					504:507	Polish Coldblood mare milk	482:507	Polish Coldblood mare milk	482:507	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30197135	12	4	theme	%	1622:1622	arg1	water					1624:1628	1% water	1621:1628	1% water	1621:1628	Addition of 1% water increased the freezing point of Polish Coldblood mare milk by 0.0069°C.					
30197135	12	5	theme	Coldblood	1669:1677	arg1	milk					1684:1687	Polish Coldblood mare milk	1662:1687	Polish Coldblood mare milk	1662:1687	Addition of 1% water increased the freezing point of Polish Coldblood mare milk by 0.0069°C.					
30197135	10	6	theme	reference	1337:1345	arg1	-0.550°C					1304:1311	-0.550°C	1304:1311	-0.550°C (from 3 to 6 mo)	1304:1328	Based on our analysis, we adopted -0.550°C (from 3 to 6 mo) as the reference value for milk from the Polish Coldblood horse.					
30197135	10	6	theme	reference	1337:1345	arg1	value					1347:1351	the reference value	1333:1351	the reference value for milk from the Polish Coldblood horse	1333:1392	Based on our analysis, we adopted -0.550°C (from 3 to 6 mo) as the reference value for milk from the Polish Coldblood horse.					
30197135	10	7	from	mo	1326:1327	arg1	-0.550°C					1304:1311	-0.550°C	1304:1311	-0.550°C (from 3 to 6 mo)	1304:1328	Based on our analysis, we adopted -0.550°C (from 3 to 6 mo) as the reference value for milk from the Polish Coldblood horse.					
30197135	10	7	from	mo	1326:1327	arg1	value					1347:1351	the reference value	1333:1351	the reference value for milk from the Polish Coldblood horse	1333:1392	Based on our analysis, we adopted -0.550°C (from 3 to 6 mo) as the reference value for milk from the Polish Coldblood horse.					
30197135	9	8	dep	percentile	1237:1246	arg1	to					1229:1230	to	1229:1230	to	1229:1230	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	10	9	theme	Polish	1371:1376	arg1	horse					1388:1392	the Polish Coldblood horse	1367:1392	the Polish Coldblood horse	1367:1392	Based on our analysis, we adopted -0.550°C (from 3 to 6 mo) as the reference value for milk from the Polish Coldblood horse.					
30197135	5	10	theme	lactation	725:733	arg1	mo					719:720	the next 9 mo	708:720	the next 9 mo of lactation (n = 52)	708:742	Their milk was tested on d 1, 3, and 7 postpartum and once a month for the next 9 mo of lactation (n = 52).					
30197135	12	11	theme	freezing	1644:1651	arg1	point					1653:1657	the freezing point	1640:1657	the freezing point of Polish Coldblood mare milk by 0.0069°C	1640:1699	Addition of 1% water increased the freezing point of Polish Coldblood mare milk by 0.0069°C.					
30197135	3	12	theme	milk	504:507	arg1	parameters					468:477	various selected technological parameters	437:477	various selected technological parameters of Polish Coldblood mare milk	437:507	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30197135	9	13	theme	mean	1132:1135	arg1	-0.557°C					1180:1187	-0.557°C	1180:1187	-0.557°C	1180:1187	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	13	theme	mean	1132:1135	arg1	coefficient					1190:1200	coefficient	1190:1200	coefficient of variation = -2.787; 5th to 95th percentile	1190:1246	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	13	theme	mean	1132:1135	arg1	point					1146:1150	The mean freezing point	1128:1150	The mean freezing point during a 9-mo lactation	1128:1174	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	14	dep	-0.550°C	1259:1266	arg1	to					1256:1257	to	1256:1257	to	1256:1257	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	5	15	theme	next	712:715	arg1	mo					719:720	the next 9 mo	708:720	the next 9 mo of lactation (n = 52)	708:742	Their milk was tested on d 1, 3, and 7 postpartum and once a month for the next 9 mo of lactation (n = 52).					
30197135	9	16	dep	=	1215:1215	arg1	percentile					1237:1246	-2.787; 5th to 95th percentile	1217:1246	-2.787; 5th to 95th percentile	1217:1246	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	11	17	dep	recommended	1574:1584	arg1	y					1587:1587	y	1587:1587	y = 79.35 + 144.35x	1587:1605	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	7	18	theme	total	962:966	arg1	g/kg					981:984	total protein 24.2 g/kg	962:984	total protein 24.2 g/kg (made up of casein, 14.6 g/kg, and whey protein, 9.5 g/kg)	962:1043	The basic composition of the milk was as follows: solids-not-fat, 84.4 g/kg, fat 15.1 g/kg, lactose 65.3 g/kg, and total protein 24.2 g/kg (made up of casein, 14.6 g/kg, and whey protein, 9.5 g/kg).					
30197135	11	19	theme	linear	1552:1557	arg1	model					1559:1563	a linear model	1550:1563	a linear model	1550:1563	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	1	20	theme	sought-after	121:132	arg1	material					138:145	sought-after raw material	121:145	sought-after raw material	121:145	Mare milk is a valued and sought-after raw material for the production of innovative dairy products.					
30197135	0	21	theme	Polish	65:70	arg1	breed					88:92	the Polish Coldblood horse breed	61:92	the Polish Coldblood horse breed	61:92	Freezing point and other technological properties of milk of the Polish Coldblood horse breed.					
30197135	7	22	dep	fat	924:926	arg1	g/kg					933:936	15.1 g/kg	928:936	fat 15.1 g/kg	924:936	The basic composition of the milk was as follows: solids-not-fat, 84.4 g/kg, fat 15.1 g/kg, lactose 65.3 g/kg, and total protein 24.2 g/kg (made up of casein, 14.6 g/kg, and whey protein, 9.5 g/kg).					
30197135	9	23	dep	-0.557°C	1180:1187	arg1	-0.557°C					1180:1187	-0.557°C	1180:1187	-0.557°C	1180:1187	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	23	dep	-0.557°C	1180:1187	arg1	point					1146:1150	The mean freezing point	1128:1150	The mean freezing point during a 9-mo lactation	1128:1174	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	23	dep	-0.557°C	1180:1187	arg1	coefficient					1190:1200	coefficient	1190:1200	coefficient of variation = -2.787; 5th to 95th percentile	1190:1246	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	0	24	theme	horse	82:86	arg1	breed					88:92	the Polish Coldblood horse breed	61:92	the Polish Coldblood horse breed	61:92	Freezing point and other technological properties of milk of the Polish Coldblood horse breed.					
30197135	9	25	theme	variation	1205:1213	arg1	-0.557°C					1180:1187	-0.557°C	1180:1187	-0.557°C	1180:1187	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	25	theme	variation	1205:1213	arg1	point					1146:1150	The mean freezing point	1128:1150	The mean freezing point during a 9-mo lactation	1128:1174	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	25	theme	variation	1205:1213	arg1	coefficient					1190:1200	coefficient	1190:1200	coefficient of variation = -2.787; 5th to 95th percentile	1190:1246	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	11	26	theme	information	1515:1525	arg1	criterion					1527:1535	Akaike's information criterion	1506:1535	Akaike's information criterion	1506:1535	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	2	27	theme	fraudulent	337:346	arg1	dilution					348:355	its deliberate fraudulent dilution	322:355	its deliberate fraudulent dilution	322:355	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	8	28	theme	dominant	1050:1057	arg1	acid					1085:1088	glutamic acid	1076:1088	glutamic acid	1076:1088	The dominant amino acids were glutamic acid, proline, leucine, and aspartic acid.					
30197135	8	28	theme	dominant	1050:1057	arg1	acids					1065:1069	The dominant amino acids	1046:1069	The dominant amino acids	1046:1069	The dominant amino acids were glutamic acid, proline, leucine, and aspartic acid.					
30197135	7	29	theme	whey	1021:1024	arg1	protein					1026:1032	whey protein	1021:1032	whey protein	1021:1032	The basic composition of the milk was as follows: solids-not-fat, 84.4 g/kg, fat 15.1 g/kg, lactose 65.3 g/kg, and total protein 24.2 g/kg (made up of casein, 14.6 g/kg, and whey protein, 9.5 g/kg).					
30197135	10	30	dep	6	1324:1324	arg1	to					1321:1322	to	1321:1322	to	1321:1322	Based on our analysis, we adopted -0.550°C (from 3 to 6 mo) as the reference value for milk from the Polish Coldblood horse.					
30197135	1	31	theme	Mare	95:98	arg1	milk					100:103	Mare milk	95:103	Mare milk	95:103	Mare milk is a valued and sought-after raw material for the production of innovative dairy products.					
30197135	8	32	theme	glutamic	1076:1083	arg1	acid					1085:1088	glutamic acid	1076:1088	glutamic acid	1076:1088	The dominant amino acids were glutamic acid, proline, leucine, and aspartic acid.					
30197135	8	32	theme	glutamic	1076:1083	arg1	acids					1065:1069	The dominant amino acids	1046:1069	The dominant amino acids	1046:1069	The dominant amino acids were glutamic acid, proline, leucine, and aspartic acid.					
30197135	0	33	theme	technological	25:37	arg1	properties					39:48	other technological properties	19:48	other technological properties of milk of the Polish Coldblood horse breed	19:92	Freezing point and other technological properties of milk of the Polish Coldblood horse breed.					
30197135	6	34	from	mares	758:762	arg1	Milk					745:748	Milk	745:748	Milk from 13 mares, taken daily in mo 4 of lactation,	745:797	Milk from 13 mares, taken daily in mo 4 of lactation, was collected for the dilution study (n = 403).					
30197135	2	35	theme	low	213:215	arg1	supply					217:222	low supply	213:222	low supply	213:222	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	2	36	theme	milk	282:285	arg1	characterization					258:273	accurate characterization	249:273	accurate characterization of the milk of a given horse breed	249:308	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	4	37	theme	multiparous	538:548	arg1	mares					567:571	multiparous Polish Coldblood mares	538:571	multiparous Polish Coldblood mares from 5 to 8 yr of age	538:593	Research was carried out on multiparous Polish Coldblood mares from 5 to 8 yr of age with live weights between 618 and 851 kg.					
30197135	3	38	theme	selected	445:452	arg1	parameters					468:477	various selected technological parameters	437:477	various selected technological parameters of Polish Coldblood mare milk	437:507	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30197135	4	39	theme	Coldblood	557:565	arg1	mares					567:571	multiparous Polish Coldblood mares	538:571	multiparous Polish Coldblood mares from 5 to 8 yr of age	538:593	Research was carried out on multiparous Polish Coldblood mares from 5 to 8 yr of age with live weights between 618 and 851 kg.					
30197135	2	40	theme	horse	298:302	arg1	breed					304:308	a given horse breed	290:308	a given horse breed	290:308	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	11	41	from	model	1411:1415	arg1	point					1440:1444	freezing point	1431:1444	freezing point	1431:1444	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	7	42	theme	milk	876:879	arg1	composition					857:867	The basic composition	847:867	The basic composition of the milk	847:879	The basic composition of the milk was as follows: solids-not-fat, 84.4 g/kg, fat 15.1 g/kg, lactose 65.3 g/kg, and total protein 24.2 g/kg (made up of casein, 14.6 g/kg, and whey protein, 9.5 g/kg).					
30197135	3	43	theme	Polish	482:487	arg1	milk					504:507	Polish Coldblood mare milk	482:507	Polish Coldblood mare milk	482:507	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30197135	11	44	from	changes	1420:1426	arg1	point					1440:1444	freezing point	1431:1444	freezing point	1431:1444	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	5	45	theme	=	738:738	arg1	n					736:736	n = 52	736:741	n = 52	736:741	Their milk was tested on d 1, 3, and 7 postpartum and once a month for the next 9 mo of lactation (n = 52).					
30197135	5	45	theme	=	738:738	arg1	lactation					725:733	lactation	725:733	lactation (n = 52)	725:742	Their milk was tested on d 1, 3, and 7 postpartum and once a month for the next 9 mo of lactation (n = 52).					
30197135	3	46	theme	parameters	468:477	arg1	background					423:432	a background	421:432	a background of various selected technological parameters of Polish Coldblood mare milk	421:507	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30197135	12	47	theme	milk	1684:1687	arg1	point					1653:1657	the freezing point	1640:1657	the freezing point of Polish Coldblood mare milk by 0.0069°C	1640:1699	Addition of 1% water increased the freezing point of Polish Coldblood mare milk by 0.0069°C.					
30197135	12	48	theme	Polish	1662:1667	arg1	milk					1684:1687	Polish Coldblood mare milk	1662:1687	Polish Coldblood mare milk	1662:1687	Addition of 1% water increased the freezing point of Polish Coldblood mare milk by 0.0069°C.					
30197135	7	49	theme	basic	851:855	arg1	composition					857:867	The basic composition	847:867	The basic composition of the milk	847:879	The basic composition of the milk was as follows: solids-not-fat, 84.4 g/kg, fat 15.1 g/kg, lactose 65.3 g/kg, and total protein 24.2 g/kg (made up of casein, 14.6 g/kg, and whey protein, 9.5 g/kg).					
30197135	10	50	theme	Coldblood	1378:1386	arg1	horse					1388:1392	the Polish Coldblood horse	1367:1392	the Polish Coldblood horse	1367:1392	Based on our analysis, we adopted -0.550°C (from 3 to 6 mo) as the reference value for milk from the Polish Coldblood horse.					
30197135	4	51	from	yr	585:586	arg1	mares					567:571	multiparous Polish Coldblood mares	538:571	multiparous Polish Coldblood mares from 5 to 8 yr of age	538:593	Research was carried out on multiparous Polish Coldblood mares from 5 to 8 yr of age with live weights between 618 and 851 kg.					
30197135	4	52	theme	age	591:593	arg1	yr					585:586	5 to 8 yr	578:586	5 to 8 yr of age	578:593	Research was carried out on multiparous Polish Coldblood mares from 5 to 8 yr of age with live weights between 618 and 851 kg.					
30197135	2	53	theme	accurate	249:256	arg1	characterization					258:273	accurate characterization	249:273	accurate characterization of the milk of a given horse breed	249:308	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	1	54	theme	innovative	169:178	arg1	products					186:193	innovative dairy products	169:193	innovative dairy products	169:193	Mare milk is a valued and sought-after raw material for the production of innovative dairy products.					
30197135	2	55	theme	characterization	258:273	arg1	demand					205:210	The high demand	196:210	The high demand	196:210	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	2	55	theme	characterization	258:273	arg1	supply					217:222	low supply	213:222	low supply	213:222	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	2	55	theme	characterization	258:273	arg1	price					230:234	high price	225:234	high price	225:234	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	2	55	theme	characterization	258:273	arg1	lack					241:244	lack	241:244	lack of accurate characterization of the milk of a given horse breed	241:308	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	8	56	theme	aspartic	1113:1120	arg1	acid					1122:1125	aspartic acid	1113:1125	aspartic acid	1113:1125	The dominant amino acids were glutamic acid, proline, leucine, and aspartic acid.					
30197135	12	57	theme	mare	1679:1682	arg1	milk					1684:1687	Polish Coldblood mare milk	1662:1687	Polish Coldblood mare milk	1662:1687	Addition of 1% water increased the freezing point of Polish Coldblood mare milk by 0.0069°C.					
30197135	9	58	theme	freezing	1137:1144	arg1	-0.557°C					1180:1187	-0.557°C	1180:1187	-0.557°C	1180:1187	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	58	theme	freezing	1137:1144	arg1	coefficient					1190:1200	coefficient	1190:1200	coefficient of variation = -2.787; 5th to 95th percentile	1190:1246	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	58	theme	freezing	1137:1144	arg1	point					1146:1150	The mean freezing point	1128:1150	The mean freezing point during a 9-mo lactation	1128:1174	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	11	59	theme	changes	1420:1426	arg1	model					1411:1415	the model	1407:1415	the model of changes in freezing point, after dilution	1407:1460	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	2	60	theme	high	200:203	arg1	demand					205:210	The high demand	196:210	The high demand	196:210	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	12	61	theme	1	1621:1621	arg1	%					1622:1622	%	1622:1622	%	1622:1622	Addition of 1% water increased the freezing point of Polish Coldblood mare milk by 0.0069°C.					
30197135	11	62	theme	model	1411:1415	arg1	Analysis					1395:1402	Analysis	1395:1402	Analysis	1395:1402	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	2	63	theme	high	225:228	arg1	price					230:234	high price	225:234	high price	225:234	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	9	64	theme	9-mo	1161:1164	arg1	lactation					1166:1174	a 9-mo lactation	1159:1174	a 9-mo lactation	1159:1174	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	11	65	theme	=	1589:1589	arg1	y					1587:1587	y	1587:1587	y = 79.35 + 144.35x	1587:1605	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	6	66	theme	dilution	821:828	arg1	n					837:837	n = 403	837:843	n = 403	837:843	Milk from 13 mares, taken daily in mo 4 of lactation, was collected for the dilution study (n = 403).					
30197135	6	66	theme	dilution	821:828	arg1	study					830:834	the dilution study	817:834	the dilution study (n = 403)	817:844	Milk from 13 mares, taken daily in mo 4 of lactation, was collected for the dilution study (n = 403).					
30197135	12	67	theme	water	1624:1628	arg1	Addition					1609:1616	Addition	1609:1616	Addition of 1% water	1609:1628	Addition of 1% water increased the freezing point of Polish Coldblood mare milk by 0.0069°C.					
30197135	11	68	dep	=	1589:1589	arg1	79.35					1591:1595	79.35	1591:1595	79.35	1591:1595	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	11	68	dep	=	1589:1589	arg1	144.35x					1599:1605	144.35x	1599:1605	144.35x	1599:1605	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	4	69	theme	live	600:603	arg1	weights					605:611	live weights	600:611	live weights between 618 and 851 kg	600:634	Research was carried out on multiparous Polish Coldblood mares from 5 to 8 yr of age with live weights between 618 and 851 kg.					
30197135	0	70	theme	Coldblood	72:80	arg1	breed					88:92	the Polish Coldblood horse breed	61:92	the Polish Coldblood horse breed	61:92	Freezing point and other technological properties of milk of the Polish Coldblood horse breed.					
30197135	1	71	theme	raw	134:136	arg1	material					138:145	sought-after raw material	121:145	sought-after raw material	121:145	Mare milk is a valued and sought-after raw material for the production of innovative dairy products.					
30197135	0	72	theme	breed	88:92	arg1	milk					53:56	milk	53:56	milk of the Polish Coldblood horse breed	53:92	Freezing point and other technological properties of milk of the Polish Coldblood horse breed.					
30197135	6	73	theme	lactation	788:796	arg1	mo					780:781	mo 4	780:783	mo 4 of lactation	780:796	Milk from 13 mares, taken daily in mo 4 of lactation, was collected for the dilution study (n = 403).					
30197135	1	74	theme	dairy	180:184	arg1	products					186:193	innovative dairy products	169:193	innovative dairy products	169:193	Mare milk is a valued and sought-after raw material for the production of innovative dairy products.					
30197135	9	75	theme	=	1215:1215	arg1	-0.557°C					1180:1187	-0.557°C	1180:1187	-0.557°C	1180:1187	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	75	theme	=	1215:1215	arg1	point					1146:1150	The mean freezing point	1128:1150	The mean freezing point during a 9-mo lactation	1128:1174	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	9	75	theme	=	1215:1215	arg1	coefficient					1190:1200	coefficient	1190:1200	coefficient of variation = -2.787; 5th to 95th percentile	1190:1246	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	10	76	from	horse	1388:1392	arg1	milk					1357:1360	milk	1357:1360	milk from the Polish Coldblood horse	1357:1392	Based on our analysis, we adopted -0.550°C (from 3 to 6 mo) as the reference value for milk from the Polish Coldblood horse.					
30197135	2	77	theme	deliberate	326:335	arg1	dilution					348:355	its deliberate fraudulent dilution	322:355	its deliberate fraudulent dilution	322:355	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	11	78	from	point	1440:1444	arg1	model					1411:1415	the model	1407:1415	the model of changes in freezing point, after dilution	1407:1460	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	9	79	dep	coefficient	1190:1200	arg1	-0.550°C					1259:1266	-0.550°C	1259:1266	-0.550°C	1259:1266	The mean freezing point during a 9-mo lactation was -0.557°C (coefficient of variation = -2.787; 5th to 95th percentile: -0.563 to -0.550°C).					
30197135	0	80	theme	other	19:23	arg1	properties					39:48	other technological properties	19:48	other technological properties of milk of the Polish Coldblood horse breed	19:92	Freezing point and other technological properties of milk of the Polish Coldblood horse breed.					
30197135	6	81	theme	=	839:839	arg1	n					837:837	n = 403	837:843	n = 403	837:843	Milk from 13 mares, taken daily in mo 4 of lactation, was collected for the dilution study (n = 403).					
30197135	6	81	theme	=	839:839	arg1	study					830:834	the dilution study	817:834	the dilution study (n = 403)	817:844	Milk from 13 mares, taken daily in mo 4 of lactation, was collected for the dilution study (n = 403).					
30197135	8	82	theme	amino	1059:1063	arg1	acid					1085:1088	glutamic acid	1076:1088	glutamic acid	1076:1088	The dominant amino acids were glutamic acid, proline, leucine, and aspartic acid.					
30197135	8	82	theme	amino	1059:1063	arg1	acids					1065:1069	The dominant amino acids	1046:1069	The dominant amino acids	1046:1069	The dominant amino acids were glutamic acid, proline, leucine, and aspartic acid.					
30197135	3	83	theme	freezing	398:405	arg1	point					407:411	the freezing point	394:411	the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk	394:507	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30197135	7	84	theme	lactose	939:945	arg1	g/kg					952:955	lactose 65.3 g/kg	939:955	lactose 65.3 g/kg	939:955	The basic composition of the milk was as follows: solids-not-fat, 84.4 g/kg, fat 15.1 g/kg, lactose 65.3 g/kg, and total protein 24.2 g/kg (made up of casein, 14.6 g/kg, and whey protein, 9.5 g/kg).					
30197135	1	85	theme	products	186:193	arg1	production					155:164	the production	151:164	the production of innovative dairy products	151:193	Mare milk is a valued and sought-after raw material for the production of innovative dairy products.					
30197135	4	86	dep	8	583:583	arg1	to					580:581	to	580:581	to	580:581	Research was carried out on multiparous Polish Coldblood mares from 5 to 8 yr of age with live weights between 618 and 851 kg.					
30197135	3	87	theme	work	374:377	arg1	aim					362:364	The aim	358:364	The aim of this work	358:377	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30197135	0	88	theme	milk	53:56	arg1	properties					39:48	other technological properties	19:48	other technological properties of milk of the Polish Coldblood horse breed	19:92	Freezing point and other technological properties of milk of the Polish Coldblood horse breed.					
30197135	0	88	theme	milk	53:56	arg1	point					9:13	point	9:13	point	9:13	Freezing point and other technological properties of milk of the Polish Coldblood horse breed.					
30197135	2	89	theme	breed	304:308	arg1	milk					282:285	the milk	278:285	the milk of a given horse breed	278:308	The high demand, low supply, high price, and lack of accurate characterization of the milk of a given horse breed may provoke its deliberate fraudulent dilution.					
30197135	3	90	theme	various	437:443	arg1	parameters					468:477	various selected technological parameters	437:477	various selected technological parameters of Polish Coldblood mare milk	437:507	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30197135	11	91	theme	freezing	1431:1438	arg1	point					1440:1444	freezing point	1431:1444	freezing point	1431:1444	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	11	92	theme	determination	1488:1500	arg1	coefficient					1473:1483	the coefficient	1469:1483	the coefficient of determination and Akaike's information criterion	1469:1535	Analysis of the model of changes in freezing point, after dilution, using the coefficient of determination and Akaike's information criterion, showed that a linear model could be recommended (y = 79.35 + 144.35x).					
30197135	3	93	theme	technological	454:466	arg1	parameters					468:477	various selected technological parameters	437:477	various selected technological parameters of Polish Coldblood mare milk	437:507	The aim of this work was to analyze the freezing point against a background of various selected technological parameters of Polish Coldblood mare milk.					
30093026	9	0	theme	various	1632:1638	arg1	research					1655:1662	various pharmaceutical research	1632:1662	various pharmaceutical research	1632:1662	In addition, mPEG and PCL are recognized as biocompatible polymers and chitosan has been engaged in various pharmaceutical research.					
30093026	6	1	from	composition	1083:1093	arg1	copolymers					1110:1119	the diblock copolymers	1098:1119	the diblock copolymers	1098:1119	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	5	2	theme	responsive	874:883	arg1	transitions					899:909	pH responsive sol-gel phase transitions	871:909	pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments	871:993	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	6	3	theme	Gelation	996:1003	arg1	Tgel					1018:1021	Tgel	1018:1021	Tgel	1018:1021	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	6	3	theme	Gelation	996:1003	arg1	temperature					1005:1015	Gelation temperature	996:1015	Gelation temperature (Tgel)	996:1022	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	9	4	theme	pharmaceutical	1640:1653	arg1	research					1655:1662	various pharmaceutical research	1632:1662	various pharmaceutical research	1632:1662	In addition, mPEG and PCL are recognized as biocompatible polymers and chitosan has been engaged in various pharmaceutical research.					
30093026	2	5	theme	poly	349:352	arg1	ether					378:382	poly(ethylene glycol) methyl ether	349:382	poly(ethylene glycol) methyl ether (mPEG)	349:389	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	2	5	theme	poly	349:352	arg1	mPEG					385:388	mPEG	385:388	mPEG	385:388	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	7	6	theme	sustained	1281:1289	arg1	release					1291:1297	the sustained release	1277:1297	the sustained release of both doxorubicin and curcumin for up to 2 weeks	1277:1348	The graft copolymer hydrogels successfully showed the sustained release of both doxorubicin and curcumin for up to 2 weeks.					
30093026	0	7	theme	gelation	99:106	arg1	temperature					108:118	tuned gelation temperature	93:118	tuned gelation temperature suitable for tumor acidic environment	93:156	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	5	8	theme	microenvironments	977:993	arg1	pH					958:959	pH	958:959	pH of acidic tumor microenvironments	958:993	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	5	8	theme	microenvironments	977:993	arg1	temperature					942:952	body temperature	937:952	body temperature	937:952	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	10	9	theme	alternative	1706:1716	arg1	choice					1718:1723	an alternative choice	1703:1723	an alternative choice for drug delivery applications	1703:1754	Thus, this system could be considered an alternative choice for drug delivery applications.					
30093026	3	10	theme	responsive	503:512	arg1	biopolymer					514:523	a pH responsive biopolymer	498:523	a pH responsive biopolymer	498:523	Subsequently, the diblock copolymers were grafted onto chitosan, a pH responsive biopolymer.					
30093026	3	10	theme	responsive	503:512	arg1	chitosan					488:495	chitosan	488:495	chitosan	488:495	Subsequently, the diblock copolymers were grafted onto chitosan, a pH responsive biopolymer.					
30093026	0	11	theme	tuned	93:97	arg1	temperature					108:118	tuned gelation temperature	93:118	tuned gelation temperature suitable for tumor acidic environment	93:156	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	0	12	from	pH	57:58	arg1	Preparation					0:10	Preparation	0:10	Preparation of injectable hydrogels from temperature and pH responsive	0:69	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	6	13	theme	copolymers	1203:1212	arg1	content					1180:1186	grafting content	1171:1186	grafting content of the diblock copolymers on chitosan	1171:1224	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	4	14	theme	tube	720:723	arg1	method					735:740	the test tube inversion method	711:740	the test tube inversion method as well as dynamic rheological measurements	711:784	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	1	15	theme	environment	259:269	arg1	temperature					233:243	temperature	233:243	temperature	233:243	In this present work, stimuli responsive polymers that can respond to the temperature and pH of the environment were prepared.					
30093026	1	15	theme	environment	259:269	arg1	pH					249:250	pH	249:250	pH	249:250	In this present work, stimuli responsive polymers that can respond to the temperature and pH of the environment were prepared.					
30093026	0	16	theme	suitable	120:127	arg1	temperature					108:118	tuned gelation temperature	93:118	tuned gelation temperature suitable for tumor acidic environment	93:156	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	4	17	dep	chitosan-graft-	532:546	arg1	mPEG-b-PCL					577:586	mPEG-b-PCL	577:586	mPEG-b-PCL	577:586	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	4	17	dep	chitosan-graft-	532:546	arg1	mPEG-block-PCL					548:561	mPEG-block-PCL	548:561	mPEG-block-PCL	548:561	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	4	17	dep	chitosan-graft-	532:546	arg1	chitosan-g-					565:575	chitosan-g-	565:575	chitosan-g-(mPEG-b-PCL)	565:587	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	6	18	theme	PCL	1079:1081	arg1	composition					1083:1093	PCL composition	1079:1093	PCL composition in the diblock copolymers	1079:1119	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	5	19	theme	mPEG-b-PCL	805:814	arg1	copolymers					823:832	These chitosan-g-(mPEG-b-PCL) graft copolymers	787:832	These chitosan-g-(mPEG-b-PCL) graft copolymers	787:832	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	6	20	theme	grafting	1171:1178	arg1	content					1180:1186	grafting content	1171:1186	grafting content of the diblock copolymers on chitosan	1171:1224	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	5	21	theme	tunable	847:853	arg1	temperature					855:865	tunable temperature	847:865	tunable temperature	847:865	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	0	22	from	temperature	41:51	arg1	Preparation					0:10	Preparation	0:10	Preparation of injectable hydrogels from temperature and pH responsive	0:69	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	7	23	theme	curcumin	1323:1330	arg1	release					1291:1297	the sustained release	1277:1297	the sustained release of both doxorubicin and curcumin for up to 2 weeks	1277:1348	The graft copolymer hydrogels successfully showed the sustained release of both doxorubicin and curcumin for up to 2 weeks.					
30093026	3	24	theme	pH	500:501	arg1	biopolymer					514:523	a pH responsive biopolymer	498:523	a pH responsive biopolymer	498:523	Subsequently, the diblock copolymers were grafted onto chitosan, a pH responsive biopolymer.					
30093026	3	24	theme	pH	500:501	arg1	chitosan					488:495	chitosan	488:495	chitosan	488:495	Subsequently, the diblock copolymers were grafted onto chitosan, a pH responsive biopolymer.					
30093026	6	25	theme	system	1060:1065	arg1	pH					1050:1051	pH	1050:1051	pH of the system	1050:1065	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	5	26	theme	sol-gel	885:891	arg1	transitions					899:909	pH responsive sol-gel phase transitions	871:909	pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments	871:993	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	10	27	theme	drug	1729:1732	arg1	applications					1743:1754	drug delivery applications	1729:1754	drug delivery applications	1729:1754	Thus, this system could be considered an alternative choice for drug delivery applications.					
30093026	2	28	theme	responsive	310:319	arg1	copolymers					329:338	temperature responsive diblock copolymers	298:338	temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL)	298:413	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	6	29	from	content	1180:1186	arg1	chitosan					1217:1224	chitosan	1217:1224	chitosan	1217:1224	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	2	30	theme	methyl	371:376	arg1	ether					378:382	poly(ethylene glycol) methyl ether	349:382	poly(ethylene glycol) methyl ether (mPEG)	349:389	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	2	30	theme	methyl	371:376	arg1	mPEG					385:388	mPEG	385:388	mPEG	385:388	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	6	31	theme	diblock	1195:1201	arg1	copolymers					1203:1212	the diblock copolymers	1191:1212	the diblock copolymers	1191:1212	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	2	32	theme	temperature	298:308	arg1	copolymers					329:338	temperature responsive diblock copolymers	298:338	temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL)	298:413	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	1	33	theme	responsive	189:198	arg1	polymers					200:207	responsive polymers	189:207	responsive polymers	189:207	In this present work, stimuli responsive polymers that can respond to the temperature and pH of the environment were prepared.					
30093026	0	34	theme	hydrogels	26:34	arg1	Preparation					0:10	Preparation	0:10	Preparation of injectable hydrogels from temperature and pH responsive	0:69	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	4	35	theme	rheological	761:771	arg1	measurements					773:784	dynamic rheological measurements	753:784	the test tube inversion method as well as dynamic rheological measurements	711:784	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	0	36	theme	tumor	133:137	arg1	environment					146:156	tumor acidic environment	133:156	tumor acidic environment	133:156	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	7	37	dep	2	1342:1342	arg1	to					1339:1340	to	1339:1340	to	1339:1340	The graft copolymer hydrogels successfully showed the sustained release of both doxorubicin and curcumin for up to 2 weeks.					
30093026	4	38	theme	test	715:718	arg1	method					735:740	the test tube inversion method	711:740	the test tube inversion method as well as dynamic rheological measurements	711:784	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	0	39	theme	injectable	15:24	arg1	hydrogels					26:34	injectable hydrogels	15:34	injectable hydrogels	15:34	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	8	40	theme	sensitive	1514:1522	arg1	moiety					1524:1529	a temperature sensitive moiety	1500:1529	a temperature sensitive moiety	1500:1529	The designed system was based on chitosan-g-(mPEG-b-PCL) graft copolymers, of which chitosan showed pH responsive properties and mPEG-b-PCL acted as a temperature sensitive moiety.					
30093026	8	40	theme	sensitive	1514:1522	arg1	mPEG-b-PCL					1480:1489	mPEG-b-PCL	1480:1489	mPEG-b-PCL	1480:1489	The designed system was based on chitosan-g-(mPEG-b-PCL) graft copolymers, of which chitosan showed pH responsive properties and mPEG-b-PCL acted as a temperature sensitive moiety.					
30093026	8	41	theme	designed	1355:1362	arg1	system					1364:1369	The designed system	1351:1369	The designed system	1351:1369	The designed system was based on chitosan-g-(mPEG-b-PCL) graft copolymers, of which chitosan showed pH responsive properties and mPEG-b-PCL acted as a temperature sensitive moiety.					
30093026	1	42	dep	temperature	233:243	arg1	the					229:231	the	229:231	the	229:231	In this present work, stimuli responsive polymers that can respond to the temperature and pH of the environment were prepared.					
30093026	1	43	theme	present	167:173	arg1	work					175:178	this present work	162:178	this present work	162:178	In this present work, stimuli responsive polymers that can respond to the temperature and pH of the environment were prepared.					
30093026	4	44	theme	graft	590:594	arg1	copolymers					596:605	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers	526:605	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers	526:605	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	7	45	theme	doxorubicin	1307:1317	arg1	release					1291:1297	the sustained release	1277:1297	the sustained release of both doxorubicin and curcumin for up to 2 weeks	1277:1348	The graft copolymer hydrogels successfully showed the sustained release of both doxorubicin and curcumin for up to 2 weeks.					
30093026	4	46	theme	chitosan-graft-	532:546	arg1	copolymers					596:605	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers	526:605	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers	526:605	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	9	47	theme	biocompatible	1576:1588	arg1	mPEG					1545:1548	mPEG	1545:1548	mPEG	1545:1548	In addition, mPEG and PCL are recognized as biocompatible polymers and chitosan has been engaged in various pharmaceutical research.					
30093026	9	47	theme	biocompatible	1576:1588	arg1	polymers					1590:1597	biocompatible polymers	1576:1597	biocompatible polymers	1576:1597	In addition, mPEG and PCL are recognized as biocompatible polymers and chitosan has been engaged in various pharmaceutical research.					
30093026	9	47	theme	biocompatible	1576:1588	arg1	PCL					1554:1556	PCL	1554:1556	PCL	1554:1556	In addition, mPEG and PCL are recognized as biocompatible polymers and chitosan has been engaged in various pharmaceutical research.					
30093026	4	48	theme	1H	642:643	arg1	NMR					645:647	1H NMR	642:647	1H NMR	642:647	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	8	49	theme	mPEG-b-PCL	1396:1405	arg1	copolymers					1414:1423	chitosan-g-(mPEG-b-PCL) graft copolymers	1384:1423	chitosan-g-(mPEG-b-PCL) graft copolymers	1384:1423	The designed system was based on chitosan-g-(mPEG-b-PCL) graft copolymers, of which chitosan showed pH responsive properties and mPEG-b-PCL acted as a temperature sensitive moiety.					
30093026	0	50	theme	acidic	139:144	arg1	environment					146:156	tumor acidic environment	133:156	tumor acidic environment	133:156	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	8	51	theme	chitosan-g-	1384:1394	arg1	copolymers					1414:1423	chitosan-g-(mPEG-b-PCL) graft copolymers	1384:1423	chitosan-g-(mPEG-b-PCL) graft copolymers	1384:1423	The designed system was based on chitosan-g-(mPEG-b-PCL) graft copolymers, of which chitosan showed pH responsive properties and mPEG-b-PCL acted as a temperature sensitive moiety.					
30093026	5	52	theme	graft	817:821	arg1	copolymers					823:832	These chitosan-g-(mPEG-b-PCL) graft copolymers	787:832	These chitosan-g-(mPEG-b-PCL) graft copolymers	787:832	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	5	53	theme	chitosan-g-	793:803	arg1	copolymers					823:832	These chitosan-g-(mPEG-b-PCL) graft copolymers	787:832	These chitosan-g-(mPEG-b-PCL) graft copolymers	787:832	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	7	54	theme	graft	1231:1235	arg1	hydrogels					1247:1255	The graft copolymer hydrogels	1227:1255	The graft copolymer hydrogels	1227:1255	The graft copolymer hydrogels successfully showed the sustained release of both doxorubicin and curcumin for up to 2 weeks.					
30093026	4	55	theme	phase	676:680	arg1	transitions					682:692	their sol-gel phase transitions	662:692	their sol-gel phase transitions	662:692	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	10	56	theme	delivery	1734:1741	arg1	applications					1743:1754	drug delivery applications	1729:1754	drug delivery applications	1729:1754	Thus, this system could be considered an alternative choice for drug delivery applications.					
30093026	6	57	theme	solution	1133:1140	arg1	concentration					1142:1154	solution concentration	1133:1154	solution concentration	1133:1154	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	4	58	theme	sol-gel	668:674	arg1	transitions					682:692	their sol-gel phase transitions	662:692	their sol-gel phase transitions	662:692	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	8	59	theme	responsive	1454:1463	arg1	properties					1465:1474	pH responsive properties	1451:1474	pH responsive properties	1451:1474	The designed system was based on chitosan-g-(mPEG-b-PCL) graft copolymers, of which chitosan showed pH responsive properties and mPEG-b-PCL acted as a temperature sensitive moiety.					
30093026	5	60	theme	tumor	971:975	arg1	microenvironments					977:993	acidic tumor microenvironments	964:993	acidic tumor microenvironments	964:993	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	2	61	theme	copolymers	329:338	arg1	series					288:293	A series	286:293	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL)	286:413	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	5	62	theme	body	937:940	arg1	temperature					942:952	body temperature	937:952	body temperature	937:952	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	5	63	theme	acidic	964:969	arg1	microenvironments					977:993	acidic tumor microenvironments	964:993	acidic tumor microenvironments	964:993	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	5	64	theme	phase	893:897	arg1	transitions					899:909	pH responsive sol-gel phase transitions	871:909	pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments	871:993	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	4	65	theme	inversion	725:733	arg1	method					735:740	the test tube inversion method	711:740	the test tube inversion method as well as dynamic rheological measurements	711:784	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	2	66	theme	diblock	321:327	arg1	copolymers					329:338	temperature responsive diblock copolymers	298:338	temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL)	298:413	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	6	67	theme	diblock	1102:1108	arg1	copolymers					1110:1119	the diblock copolymers	1098:1119	the diblock copolymers	1098:1119	Gelation temperature (Tgel) decreased with increasing pH of the system, increasing PCL composition in the diblock copolymers, increasing solution concentration and decreasing grafting content of the diblock copolymers on chitosan.					
30093026	8	68	theme	graft	1408:1412	arg1	copolymers					1414:1423	chitosan-g-(mPEG-b-PCL) graft copolymers	1384:1423	chitosan-g-(mPEG-b-PCL) graft copolymers	1384:1423	The designed system was based on chitosan-g-(mPEG-b-PCL) graft copolymers, of which chitosan showed pH responsive properties and mPEG-b-PCL acted as a temperature sensitive moiety.					
30093026	5	69	theme	pH	871:872	arg1	transitions					899:909	pH responsive sol-gel phase transitions	871:909	pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments	871:993	These chitosan-g-(mPEG-b-PCL) graft copolymers demonstrated tunable temperature and pH responsive sol-gel phase transitions that correspond well with body temperature and pH of acidic tumor microenvironments.					
30093026	1	70	dep	polymers	200:207	arg1	stimuli					181:187	stimuli	181:187	stimuli responsive polymers that can respond to the temperature and pH of the environment	181:269	In this present work, stimuli responsive polymers that can respond to the temperature and pH of the environment were prepared.					
30093026	2	71	theme	glycol	363:368	arg1	ether					378:382	poly(ethylene glycol) methyl ether	349:382	poly(ethylene glycol) methyl ether (mPEG)	349:389	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	2	71	theme	glycol	363:368	arg1	mPEG					385:388	mPEG	385:388	mPEG	385:388	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	3	72	theme	diblock	451:457	arg1	copolymers					459:468	the diblock copolymers	447:468	the diblock copolymers	447:468	Subsequently, the diblock copolymers were grafted onto chitosan, a pH responsive biopolymer.					
30093026	0	73	theme	responsive	60:69	arg1	pH					57:58	pH responsive	57:69	pH responsive	57:69	Preparation of injectable hydrogels from temperature and pH responsive grafted chitosan with tuned gelation temperature suitable for tumor acidic environment.					
30093026	4	74	theme	dynamic	753:759	arg1	measurements					773:784	dynamic rheological measurements	753:784	the test tube inversion method as well as dynamic rheological measurements	711:784	These chitosan-graft-(mPEG-block-PCL) (chitosan-g-(mPEG-b-PCL)) graft copolymers were structurally characterized by 1H NMR and FTIR and their sol-gel phase transitions were analyzed by the test tube inversion method as well as dynamic rheological measurements.					
30093026	2	75	theme	ethylene	354:361	arg1	ether					378:382	poly(ethylene glycol) methyl ether	349:382	poly(ethylene glycol) methyl ether (mPEG)	349:389	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	2	75	theme	ethylene	354:361	arg1	mPEG					385:388	mPEG	385:388	mPEG	385:388	A series of temperature responsive diblock copolymers based on poly(ethylene glycol) methyl ether (mPEG) and ε-caprolactone (CL) were synthesized.					
30093026	7	76	theme	copolymer	1237:1245	arg1	hydrogels					1247:1255	The graft copolymer hydrogels	1227:1255	The graft copolymer hydrogels	1227:1255	The graft copolymer hydrogels successfully showed the sustained release of both doxorubicin and curcumin for up to 2 weeks.					
31108840	3	0	theme	intake	331:336	arg1	effects					307:313	the effects	303:313	the effects of high sucrose intake on lung mechanics and alveolar septal composition	303:386	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	4	1	theme	%	491:491	arg1	sucrose					493:499	7% sucrose	490:499	7% sucrose	490:499	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	8	2	theme	alveolar	960:967	arg1	volumes					980:986	normal alveolar epithelial volumes	953:986	normal alveolar epithelial volumes	953:986	Lung and septal volumes were increased and the septal ratio of elastic-to-collagen fibers was decreased despite normal alveolar epithelial volumes.					
31108840	6	3	theme	lung	636:639	arg1	mechanics					641:649	lung mechanics	636:649	lung mechanics	636:649	After 30 weeks, lung mechanics were assessed, left lungs were used for stereological analysis and right lungs for protein expression measurement.					
31108840	1	4	theme	comorbid	159:166	arg1	Diabetes					110:117	Diabetes	110:117	Diabetes	110:117	Diabetes and respiratory diseases are frequently comorbid conditions.					
31108840	1	4	theme	comorbid	159:166	arg1	diseases					135:142	respiratory diseases	123:142	respiratory diseases	123:142	Diabetes and respiratory diseases are frequently comorbid conditions.					
31108840	1	4	theme	comorbid	159:166	arg1	conditions					168:177	comorbid conditions	159:177	comorbid conditions	159:177	Diabetes and respiratory diseases are frequently comorbid conditions.					
31108840	9	5	theme	Elastic	989:995	arg1	fibers					997:1002	Elastic fibers	989:1002	Elastic fibers	989:1002	Elastic fibers appeared more loosely arranged accompanied by an increase in elastin protein expression.					
31108840	6	6	theme	expression	742:751	arg1	measurement					753:763	protein expression measurement	734:763	protein expression measurement	734:763	After 30 weeks, lung mechanics were assessed, left lungs were used for stereological analysis and right lungs for protein expression measurement.					
31108840	12	7	theme	matrix	1253:1258	arg1	similar					1337:1343	similar	1337:1343	similar	1337:1343	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	12	7	theme	matrix	1253:1258	arg1	composition					1266:1276	The septal extracellular matrix (ECM) composition	1228:1276	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components	1228:1331	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	4	8	theme	%	533:533	arg1	HSD					526:528	HSD	526:528	HSD	526:528	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	4	8	theme	%	533:533	arg1	sucrose					535:541	35% sucrose	531:541	35% sucrose	531:541	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	0	9	theme	Mouse	98:102	arg1	Lung					104:107	the Mouse Lung	94:107	the Mouse Lung	94:107	Voluntary Activity Modulates Sugar-Induced Elastic Fiber Remodeling in the Alveolar Region of the Mouse Lung.					
31108840	6	10	theme	stereological	691:703	arg1	analysis					705:712	stereological analysis	691:712	stereological analysis	691:712	After 30 weeks, lung mechanics were assessed, left lungs were used for stereological analysis and right lungs for protein expression measurement.					
31108840	8	11	theme	septal	888:893	arg1	ratio					895:899	the septal ratio	884:899	the septal ratio of elastic-to-collagen fibers	884:929	Lung and septal volumes were increased and the septal ratio of elastic-to-collagen fibers was decreased despite normal alveolar epithelial volumes.					
31108840	12	12	theme	control	1348:1354	arg1	levels					1356:1361	control levels	1348:1361	control levels in the HSD-A-group	1348:1380	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	7	13	theme	higher	800:805	arg1	compliance					814:823	higher static compliance	800:823	higher static compliance	800:823	HSD resulted in hyperglycemia and higher static compliance compared to CD.					
31108840	12	14	theme	components	1322:1331	arg1	expression					1304:1313	the protein expression	1292:1313	the protein expression of ECM components	1292:1331	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	8	15	theme	epithelial	969:978	arg1	volumes					980:986	normal alveolar epithelial volumes	953:986	normal alveolar epithelial volumes	953:986	Lung and septal volumes were increased and the septal ratio of elastic-to-collagen fibers was decreased despite normal alveolar epithelial volumes.					
31108840	6	16	theme	right	718:722	arg1	lungs					724:728	right lungs	718:728	right lungs	718:728	After 30 weeks, lung mechanics were assessed, left lungs were used for stereological analysis and right lungs for protein expression measurement.					
31108840	8	17	theme	fibers	924:929	arg1	ratio					895:899	the septal ratio	884:899	the septal ratio of elastic-to-collagen fibers	884:929	Lung and septal volumes were increased and the septal ratio of elastic-to-collagen fibers was decreased despite normal alveolar epithelial volumes.					
31108840	12	18	from	levels	1356:1361	arg1	HSD-A-group					1370:1380	the HSD-A-group	1366:1380	the HSD-A-group	1366:1380	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	6	19	used	used	682:685	arg2	lungs					671:675	left lungs	666:675	left lungs	666:675	After 30 weeks, lung mechanics were assessed, left lungs were used for stereological analysis and right lungs for protein expression measurement.					
31108840	0	20	theme	Lung	104:107	arg1	Region					84:89	the Alveolar Region	71:89	the Alveolar Region of the Mouse Lung	71:107	Voluntary Activity Modulates Sugar-Induced Elastic Fiber Remodeling in the Alveolar Region of the Mouse Lung.					
31108840	4	21	theme	7	490:490	arg1	%					491:491	%	491:491	%	491:491	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	0	22	theme	Fiber	51:55	arg1	Remodeling					57:66	Sugar-Induced Elastic Fiber Remodeling	29:66	Sugar-Induced Elastic Fiber Remodeling	29:66	Voluntary Activity Modulates Sugar-Induced Elastic Fiber Remodeling in the Alveolar Region of the Mouse Lung.					
31108840	9	23	theme	protein	1073:1079	arg1	expression					1081:1090	elastin protein expression	1065:1090	elastin protein expression	1065:1090	Elastic fibers appeared more loosely arranged accompanied by an increase in elastin protein expression.					
31108840	5	24	contain	had	558:560	arg2	access					562:567	access	562:567	access to running wheels	562:585	Some animals had access to running wheels (voluntary active; CD-A, HSD-A).					
31108840	5	24	contain	had	558:560	arg1	animals					550:556	Some animals	545:556	Some animals	545:556	Some animals had access to running wheels (voluntary active; CD-A, HSD-A).					
31108840	3	25	theme	voluntary	399:407	arg1	activity					409:416	voluntary activity	399:416	voluntary activity	399:416	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	3	25	theme	voluntary	399:407	arg1	strategy					437:444	an intervention strategy	421:444	an intervention strategy	421:444	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	0	26	theme	Alveolar	75:82	arg1	Region					84:89	the Alveolar Region	71:89	the Alveolar Region of the Mouse Lung	71:107	Voluntary Activity Modulates Sugar-Induced Elastic Fiber Remodeling in the Alveolar Region of the Mouse Lung.					
31108840	11	27	theme	airspace	1169:1176	arg1	volume					1178:1183	The parenchymal airspace volume	1153:1183	The parenchymal airspace volume	1153:1183	The parenchymal airspace volume, but not the septal volume, was increased.					
31108840	13	28	theme	reduced	1451:1457	arg1	elasticity					1469:1478	reduced pulmonary elasticity	1451:1478	reduced pulmonary elasticity	1451:1478	In conclusion, HSD was associated with elastic fiber remodeling and reduced pulmonary elasticity.					
31108840	0	29	theme	Voluntary	0:8	arg1	Activity					10:17	Voluntary Activity	0:17	Voluntary Activity	0:17	Voluntary Activity Modulates Sugar-Induced Elastic Fiber Remodeling in the Alveolar Region of the Mouse Lung.					
31108840	14	30	theme	HSD-induced	1511:1521	arg1	alterations					1527:1537	HSD-induced ECM alterations	1511:1537	HSD-induced ECM alterations	1511:1537	Voluntary activity alleviated HSD-induced ECM alterations, possibly by preventing hyperglycemia.					
31108840	6	31	theme	protein	734:740	arg1	measurement					753:763	protein expression measurement	734:763	protein expression measurement	734:763	After 30 weeks, lung mechanics were assessed, left lungs were used for stereological analysis and right lungs for protein expression measurement.					
31108840	12	32	theme	ECM	1318:1320	arg1	components					1322:1331	ECM components	1318:1331	ECM components	1318:1331	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	8	33	theme	normal	953:958	arg1	volumes					980:986	normal alveolar epithelial volumes	953:986	normal alveolar epithelial volumes	953:986	Lung and septal volumes were increased and the septal ratio of elastic-to-collagen fibers was decreased despite normal alveolar epithelial volumes.					
31108840	0	34	theme	Sugar-Induced	29:41	arg1	Remodeling					57:66	Sugar-Induced Elastic Fiber Remodeling	29:66	Sugar-Induced Elastic Fiber Remodeling	29:66	Voluntary Activity Modulates Sugar-Induced Elastic Fiber Remodeling in the Alveolar Region of the Mouse Lung.					
31108840	14	35	theme	ECM	1523:1525	arg1	alterations					1527:1537	HSD-induced ECM alterations	1511:1537	HSD-induced ECM alterations	1511:1537	Voluntary activity alleviated HSD-induced ECM alterations, possibly by preventing hyperglycemia.					
31108840	12	36	theme	protein	1296:1302	arg1	expression					1304:1313	the protein expression	1292:1313	the protein expression of ECM components	1292:1331	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	5	37	dep	active	598:603	arg1	HSD-A					612:616	HSD-A	612:616	HSD-A	612:616	Some animals had access to running wheels (voluntary active; CD-A, HSD-A).					
31108840	5	37	dep	active	598:603	arg1	CD-A					606:609	CD-A	606:609	CD-A	606:609	Some animals had access to running wheels (voluntary active; CD-A, HSD-A).					
31108840	4	38	theme	35	531:532	arg1	%					533:533	%	533:533	%	533:533	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	11	39	theme	parenchymal	1157:1167	arg1	volume					1178:1183	The parenchymal airspace volume	1153:1183	The parenchymal airspace volume	1153:1183	The parenchymal airspace volume, but not the septal volume, was increased.					
31108840	9	40	dep	appeared	1004:1011	arg1	arranged					1026:1033	arranged	1026:1033	appeared more loosely arranged accompanied by an increase in elastin protein expression	1004:1090	Elastic fibers appeared more loosely arranged accompanied by an increase in elastin protein expression.					
31108840	3	41	theme	intervention	424:435	arg1	activity					409:416	voluntary activity	399:416	voluntary activity	399:416	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	3	41	theme	intervention	424:435	arg1	strategy					437:444	an intervention strategy	421:444	an intervention strategy	421:444	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	13	42	theme	elastic	1422:1428	arg1	remodeling					1436:1445	elastic fiber remodeling	1422:1445	elastic fiber remodeling	1422:1445	In conclusion, HSD was associated with elastic fiber remodeling and reduced pulmonary elasticity.					
31108840	9	43	from	increase	1053:1060	arg1	expression					1081:1090	elastin protein expression	1065:1090	elastin protein expression	1065:1090	Elastic fibers appeared more loosely arranged accompanied by an increase in elastin protein expression.					
31108840	13	44	theme	pulmonary	1459:1467	arg1	elasticity					1469:1478	reduced pulmonary elasticity	1451:1478	reduced pulmonary elasticity	1451:1478	In conclusion, HSD was associated with elastic fiber remodeling and reduced pulmonary elasticity.					
31108840	12	45	theme	septal	1232:1237	arg1	similar					1337:1343	similar	1337:1343	similar	1337:1343	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	12	45	theme	septal	1232:1237	arg1	composition					1266:1276	The septal extracellular matrix (ECM) composition	1228:1276	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components	1228:1331	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	0	46	theme	Elastic	43:49	arg1	Remodeling					57:66	Sugar-Induced Elastic Fiber Remodeling	29:66	Sugar-Induced Elastic Fiber Remodeling	29:66	Voluntary Activity Modulates Sugar-Induced Elastic Fiber Remodeling in the Alveolar Region of the Mouse Lung.					
31108840	4	47	theme	high	507:510	arg1	diet					520:523	a high sucrose diet	505:523	a high sucrose diet (HSD, 35% sucrose)	505:542	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	14	48	theme	Voluntary	1481:1489	arg1	activity					1491:1498	Voluntary activity	1481:1498	Voluntary activity	1481:1498	Voluntary activity alleviated HSD-induced ECM alterations, possibly by preventing hyperglycemia.					
31108840	9	49	theme	elastin	1065:1071	arg1	expression					1081:1090	elastin protein expression	1065:1090	elastin protein expression	1065:1090	Elastic fibers appeared more loosely arranged accompanied by an increase in elastin protein expression.					
31108840	3	50	theme	alveolar	360:367	arg1	composition					376:386	alveolar septal composition	360:386	alveolar septal composition	360:386	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	3	51	theme	septal	369:374	arg1	composition					376:386	alveolar septal composition	360:386	alveolar septal composition	360:386	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	3	52	theme	high	318:321	arg1	intake					331:336	high sucrose intake	318:336	high sucrose intake	318:336	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	4	53	theme	control	472:478	arg1	CD					486:487	CD	486:487	CD	486:487	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	4	53	theme	control	472:478	arg1	diet					480:483	a control diet	470:483	a control diet (CD, 7% sucrose)	470:500	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	2	54	theme	lung	237:240	arg1	dysfunction					242:252	lung dysfunction	237:252	lung dysfunction	237:252	However, the mechanistic links between hyperglycemia and lung dysfunction are not entirely understood.					
31108840	8	55	theme	Lung	841:844	arg1	volumes					857:863	Lung and septal volumes	841:863	volumes	857:863	Lung and septal volumes were increased and the septal ratio of elastic-to-collagen fibers was decreased despite normal alveolar epithelial volumes.					
31108840	3	56	theme	sucrose	323:329	arg1	intake					331:336	high sucrose intake	318:336	high sucrose intake	318:336	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	4	57	theme	C57BL/6N	447:454	arg1	mice					456:459	C57BL/6N mice	447:459	C57BL/6N mice	447:459	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	5	58	theme	running	572:578	arg1	wheels					580:585	running wheels	572:585	running wheels	572:585	Some animals had access to running wheels (voluntary active; CD-A, HSD-A).					
31108840	6	59	theme	left	666:669	arg1	lungs					671:675	left lungs	666:675	left lungs	666:675	After 30 weeks, lung mechanics were assessed, left lungs were used for stereological analysis and right lungs for protein expression measurement.					
31108840	5	60	dep	had	558:560	arg1	active					598:603	active	598:603	active	598:603	Some animals had access to running wheels (voluntary active; CD-A, HSD-A).					
31108840	4	61	theme	sucrose	512:518	arg1	diet					520:523	a high sucrose diet	505:523	a high sucrose diet (HSD, 35% sucrose)	505:542	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	11	62	theme	septal	1198:1203	arg1	volume					1205:1210	the septal volume	1194:1210	the septal volume	1194:1210	The parenchymal airspace volume, but not the septal volume, was increased.					
31108840	8	63	theme	elastic-to-collagen	904:922	arg1	fibers					924:929	elastic-to-collagen fibers	904:929	elastic-to-collagen fibers	904:929	Lung and septal volumes were increased and the septal ratio of elastic-to-collagen fibers was decreased despite normal alveolar epithelial volumes.					
31108840	8	64	theme	septal	850:855	arg1	volumes					857:863	Lung and septal volumes	841:863	volumes	857:863	Lung and septal volumes were increased and the septal ratio of elastic-to-collagen fibers was decreased despite normal alveolar epithelial volumes.					
31108840	1	65	theme	respiratory	123:133	arg1	Diabetes					110:117	Diabetes	110:117	Diabetes	110:117	Diabetes and respiratory diseases are frequently comorbid conditions.					
31108840	1	65	theme	respiratory	123:133	arg1	diseases					135:142	respiratory diseases	123:142	respiratory diseases	123:142	Diabetes and respiratory diseases are frequently comorbid conditions.					
31108840	1	65	theme	respiratory	123:133	arg1	conditions					168:177	comorbid conditions	159:177	comorbid conditions	159:177	Diabetes and respiratory diseases are frequently comorbid conditions.					
31108840	3	66	from	effects	307:313	arg1	mechanics					346:354	lung mechanics	341:354	lung mechanics	341:354	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	3	66	from	effects	307:313	arg1	composition					376:386	alveolar septal composition	360:386	alveolar septal composition	360:386	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	4	67	dep	CD	486:487	arg1	sucrose					493:499	7% sucrose	490:499	7% sucrose	490:499	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	9	68	dep	arranged	1026:1033	arg1	accompanied					1035:1045	accompanied	1035:1045	accompanied by an increase in elastin protein expression	1035:1090	Elastic fibers appeared more loosely arranged accompanied by an increase in elastin protein expression.					
31108840	10	69	theme	Voluntary	1093:1101	arg1	activity					1103:1110	Voluntary activity	1093:1110	Voluntary activity	1093:1110	Voluntary activity prevented hyperglycemia in HSD-fed mice.					
31108840	3	70	theme	lung	341:344	arg1	mechanics					346:354	lung mechanics	341:354	lung mechanics	341:354	This study examined the effects of high sucrose intake on lung mechanics and alveolar septal composition and tested voluntary activity as an intervention strategy.					
31108840	2	71	theme	mechanistic	193:203	arg1	links					205:209	the mechanistic links	189:209	the mechanistic links between hyperglycemia and lung dysfunction	189:252	However, the mechanistic links between hyperglycemia and lung dysfunction are not entirely understood.					
31108840	12	72	theme	extracellular	1239:1251	arg1	matrix					1253:1258	extracellular matrix	1239:1258	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components	1228:1331	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	12	72	theme	extracellular	1239:1251	arg1	ECM					1261:1263	ECM	1261:1263	ECM	1261:1263	The septal extracellular matrix (ECM) composition together with the protein expression of ECM components was similar to control levels in the HSD-A-group.					
31108840	10	73	theme	HSD-fed	1139:1145	arg1	mice					1147:1150	HSD-fed mice	1139:1150	HSD-fed mice	1139:1150	Voluntary activity prevented hyperglycemia in HSD-fed mice.					
31108840	4	74	dep	diet	520:523	arg1	HSD					526:528	HSD	526:528	HSD	526:528	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	4	74	dep	diet	520:523	arg1	sucrose					535:541	35% sucrose	531:541	35% sucrose	531:541	C57BL/6N mice were fed a control diet (CD, 7% sucrose) or a high sucrose diet (HSD, 35% sucrose).					
31108840	13	75	theme	fiber	1430:1434	arg1	remodeling					1436:1445	elastic fiber remodeling	1422:1445	elastic fiber remodeling	1422:1445	In conclusion, HSD was associated with elastic fiber remodeling and reduced pulmonary elasticity.					
31108840	7	76	theme	static	807:812	arg1	compliance					814:823	higher static compliance	800:823	higher static compliance	800:823	HSD resulted in hyperglycemia and higher static compliance compared to CD.					
30977359	7	0	theme	μm	1277:1278	arg1	diameter					1254:1261	the larger average diameter	1235:1261	the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1	1235:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	7	1	theme	surface	1194:1200	arg1	area					1202:1205	a greater specific surface area	1175:1205	a greater specific surface area (33.6 m2 g-1)	1175:1219	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	7	1	theme	surface	1194:1200	arg1	g-1					1216:1218	33.6 m2 g-1	1208:1218	33.6 m2 g-1	1208:1218	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	2	2	theme	coagulation	410:420	arg1	time					422:425	2.4 times faster plasma coagulation time	386:425	2.4 times faster plasma coagulation time	386:425	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	6	3	theme	X-ray	971:975	arg1	diffraction					977:987	X-ray diffraction	971:987	X-ray diffraction	971:987	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	4	4	theme	blood	650:654	arg1	times					660:664	whole blood 1.3 times	644:664	whole blood 1.3 times faster than the QCG	644:684	The CHNFs also coagulate whole blood 1.3 times faster than the QCG and retain twice the clotting performance after washing.					
30977359	7	5	theme	greater	1177:1183	arg1	area					1202:1205	a greater specific surface area	1175:1205	a greater specific surface area (33.6 m2 g-1)	1175:1219	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	7	5	theme	greater	1177:1183	arg1	g-1					1216:1218	33.6 m2 g-1	1208:1218	33.6 m2 g-1	1208:1218	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	1	6	theme	partial	317:323	arg1	time					340:343	activated partial thromboplastin time	307:343	activated partial thromboplastin time	307:343	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	9	7	theme	bleeding	1697:1704	arg1	episodes					1706:1713	unexpected bleeding episodes	1686:1713	unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders	1686:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	6	8	theme	energy-dispersive	933:949	arg1	spectroscopy					957:968	energy-dispersive X-ray spectroscopy	933:968	energy-dispersive X-ray spectroscopy	933:968	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	1	9	theme	one-step	220:227	arg1	process					253:259	a one-step wet-wet electrospinning process	218:259	a one-step wet-wet electrospinning process	218:259	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	2	10	theme	novel	352:356	arg1	CHNFs					372:376	These novel biocompatible CHNFs	346:376	These novel biocompatible CHNFs	346:376	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	1	11	theme	electrospinning	237:251	arg1	process					253:259	a one-step wet-wet electrospinning process	218:259	a one-step wet-wet electrospinning process	218:259	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	9	12	theme	potential	1517:1525	arg1	material					1538:1545	a potential lifesaving material	1515:1545	a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders	1515:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	9	13	theme	hereditary	1742:1751	arg1	disorders					1768:1776	hereditary blood clotting disorders	1742:1776	hereditary blood clotting disorders	1742:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	5	14	from	effective	772:780	arg1	coagulation					792:802	plasma coagulation	785:802	plasma coagulation	785:802	Halloysite clay is also more effective in plasma coagulation than commercial kaolin clay.					
30977359	9	15	theme	clotting	1759:1766	arg1	disorders					1768:1776	hereditary blood clotting disorders	1742:1776	hereditary blood clotting disorders	1742:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	0	16	from	Reduction	75:83	arg1	Time					113:116	Human Plasma Coagulation Time	88:116	Human Plasma Coagulation Time	88:116	Novel Cellulose-Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time.					
30977359	7	17	theme	small	1128:1132	arg1	diameter					1142:1149	their small average diameter	1122:1149	their small average diameter of 450 ± 260 nm	1122:1165	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	10	18	theme	bleeding	1826:1833	arg1	minutes					1809:1815	minutes	1809:1815	minutes of heavy bleeding	1809:1833	Since a person can die within minutes of heavy bleeding, every second counts for stopping traumatic hemorrhaging.					
30977359	10	18	theme	bleeding	1826:1833	arg1	counts					1849:1854	every second counts	1836:1854	every second counts for stopping traumatic hemorrhaging	1836:1890	Since a person can die within minutes of heavy bleeding, every second counts for stopping traumatic hemorrhaging.					
30977359	7	19	theme	greater	1088:1094	arg1	loading					1101:1107	a 7-fold greater clay loading	1079:1107	a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm	1079:1165	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	1	20	theme	hemostatic	157:166	arg1	CHNFs					190:194	CHNFs	190:194	CHNFs	190:194	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	1	20	theme	hemostatic	157:166	arg1	fibers					182:187	High-performance cellulose-halloysite hemostatic nanocomposite fibers	119:187	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs)	119:195	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	6	21	theme	surface	1013:1019	arg1	analysis					1026:1033	Brunauer-Emmett-Teller surface area analysis	990:1033	Brunauer-Emmett-Teller surface area analysis	990:1033	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	5	22	theme	Halloysite	743:752	arg1	clay					754:757	Halloysite clay	743:757	Halloysite clay	743:757	Halloysite clay is also more effective in plasma coagulation than commercial kaolin clay.					
30977359	9	23	theme	lifesaving	1527:1536	arg1	material					1538:1545	a potential lifesaving material	1515:1545	a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders	1515:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	5	24	theme	commercial	809:818	arg1	clay					827:830	commercial kaolin clay	809:830	commercial kaolin clay	809:830	Halloysite clay is also more effective in plasma coagulation than commercial kaolin clay.					
30977359	0	25	theme	Human	88:92	arg1	Time					113:116	Human Plasma Coagulation Time	88:116	Human Plasma Coagulation Time	88:116	Novel Cellulose-Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time.					
30977359	9	26	theme	rapid	1562:1566	arg1	loss					1574:1577	rapid blood loss	1562:1577	rapid blood loss	1562:1577	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	0	27	theme	Coagulation	101:111	arg1	Time					113:116	Human Plasma Coagulation Time	88:116	Human Plasma Coagulation Time	88:116	Novel Cellulose-Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time.					
30977359	9	28	theme	loss	1574:1577	arg1	victims					1551:1557	victims	1551:1557	victims of rapid blood loss such as military personnel	1551:1604	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	9	28	theme	loss	1574:1577	arg1	personnel					1596:1604	military personnel	1587:1604	military personnel	1587:1604	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	9	28	theme	loss	1574:1577	arg1	patients					1610:1617	patients	1610:1617	patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders	1610:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	2	29	theme	standard	459:466	arg1	QCG					491:493	QCG	491:493	QCG	491:493	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	2	29	theme	standard	459:466	arg1	Gauze					484:488	the industry gold standard QuikClot Combat Gauze	441:488	the industry gold standard QuikClot Combat Gauze (QCG)	441:494	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	7	30	theme	larger	1239:1244	arg1	diameter					1254:1261	the larger average diameter	1235:1261	the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1	1235:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	8	31	theme	human	1376:1380	arg1	fibroblasts					1390:1400	human primary fibroblasts	1376:1400	human primary fibroblasts	1376:1400	The CHNFs were shown to be noncytotoxic and human primary fibroblasts proliferated on the composite material.					
30977359	2	32	theme	industry	445:452	arg1	QCG					491:493	QCG	491:493	QCG	491:493	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	2	32	theme	industry	445:452	arg1	Gauze					484:488	the industry gold standard QuikClot Combat Gauze	441:488	the industry gold standard QuikClot Combat Gauze (QCG)	441:494	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	0	33	theme	Novel	0:4	arg1	Nanocomposite					38:50	Novel Cellulose-Halloysite Hemostatic Nanocomposite	0:50	Novel Cellulose-Halloysite Hemostatic Nanocomposite	0:50	Novel Cellulose-Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time.					
30977359	1	34	theme	thromboplastin	325:338	arg1	time					340:343	activated partial thromboplastin time	307:343	activated partial thromboplastin time	307:343	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	7	35	dep	show	1074:1077	arg1	affords					1167:1173	affords	1167:1173	show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1	1074:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	3	36	theme	superior	512:519	arg1	property					534:541	superior antileaching property	512:541	superior antileaching property of clay	512:549	The CHNFs have superior antileaching property of clay with 3 times higher post-wetting clotting activity compared to QCG.					
30977359	0	37	theme	Hemostatic	27:36	arg1	Nanocomposite					38:50	Novel Cellulose-Halloysite Hemostatic Nanocomposite	0:50	Novel Cellulose-Halloysite Hemostatic Nanocomposite	0:50	Novel Cellulose-Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time.					
30977359	1	38	theme	High-performance	119:134	arg1	CHNFs					190:194	CHNFs	190:194	CHNFs	190:194	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	1	38	theme	High-performance	119:134	arg1	fibers					182:187	High-performance cellulose-halloysite hemostatic nanocomposite fibers	119:187	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs)	119:195	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	7	39	theme	g-1	1327:1329	arg1	area					1312:1315	a specific surface area	1293:1315	a specific surface area of 1.6 m2 g-1	1293:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	2	40	theme	Combat	477:482	arg1	QCG					491:493	QCG	491:493	QCG	491:493	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	2	40	theme	Combat	477:482	arg1	Gauze					484:488	the industry gold standard QuikClot Combat Gauze	441:488	the industry gold standard QuikClot Combat Gauze (QCG)	441:494	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	7	41	theme	m2	1213:1214	arg1	area					1202:1205	a greater specific surface area	1175:1205	a greater specific surface area (33.6 m2 g-1)	1175:1219	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	7	41	theme	m2	1213:1214	arg1	g-1					1216:1218	33.6 m2 g-1	1208:1218	33.6 m2 g-1	1208:1218	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	6	42	theme	electron	912:919	arg1	microscopy					921:930	scanning electron microscopy	903:930	scanning electron microscopy	903:930	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	9	43	theme	major	1630:1634	arg1	procedures					1645:1654	major surgical procedures	1630:1654	major surgical procedures	1630:1654	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	8	44	theme	composite	1422:1430	arg1	material					1432:1439	the composite material	1418:1439	the composite material	1418:1439	The CHNFs were shown to be noncytotoxic and human primary fibroblasts proliferated on the composite material.					
30977359	2	45	theme	plasma	403:408	arg1	time					422:425	2.4 times faster plasma coagulation time	386:425	2.4 times faster plasma coagulation time	386:425	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	7	46	theme	specific	1295:1302	arg1	area					1312:1315	a specific surface area	1293:1315	a specific surface area of 1.6 m2 g-1	1293:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	3	47	theme	higher	564:569	arg1	activity					593:600	3 times higher post-wetting clotting activity	556:600	3 times higher post-wetting clotting activity	556:600	The CHNFs have superior antileaching property of clay with 3 times higher post-wetting clotting activity compared to QCG.					
30977359	1	48	theme	human	279:283	arg1	coagulation					292:302	human plasma coagulation	279:302	human plasma coagulation	279:302	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	6	49	theme	Brunauer-Emmett-Teller	990:1011	arg1	analysis					1026:1033	Brunauer-Emmett-Teller surface area analysis	990:1033	Brunauer-Emmett-Teller surface area analysis	990:1033	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	9	50	from	reduction	1454:1462	arg1	time					1479:1482	coagulation time	1467:1482	coagulation time	1467:1482	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	4	51	theme	faster	666:671	arg1	times					660:664	whole blood 1.3 times	644:664	whole blood 1.3 times faster than the QCG	644:684	The CHNFs also coagulate whole blood 1.3 times faster than the QCG and retain twice the clotting performance after washing.					
30977359	5	52	theme	kaolin	820:825	arg1	clay					827:830	commercial kaolin clay	809:830	commercial kaolin clay	809:830	Halloysite clay is also more effective in plasma coagulation than commercial kaolin clay.					
30977359	7	53	theme	specific	1185:1192	arg1	area					1202:1205	a greater specific surface area	1175:1205	a greater specific surface area (33.6 m2 g-1)	1175:1219	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	7	53	theme	specific	1185:1192	arg1	g-1					1216:1218	33.6 m2 g-1	1208:1218	33.6 m2 g-1	1208:1218	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	0	54	with	Fibers	52:57	arg1	Reduction					75:83	a Dramatic Reduction	64:83	a Dramatic Reduction in Human Plasma Coagulation Time	64:116	Novel Cellulose-Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time.					
30977359	7	55	with	QCG	1284:1286	arg1	area					1312:1315	a specific surface area	1293:1315	a specific surface area of 1.6 m2 g-1	1293:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	1	56	theme	activated	307:315	arg1	time					340:343	activated partial thromboplastin time	307:343	activated partial thromboplastin time	307:343	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	9	57	theme	unexpected	1686:1695	arg1	episodes					1706:1713	unexpected bleeding episodes	1686:1713	unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders	1686:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	4	58	theme	whole	644:648	arg1	times					660:664	whole blood 1.3 times	644:664	whole blood 1.3 times faster than the QCG	644:684	The CHNFs also coagulate whole blood 1.3 times faster than the QCG and retain twice the clotting performance after washing.					
30977359	3	59	theme	clay	546:549	arg1	property					534:541	superior antileaching property	512:541	superior antileaching property of clay	512:549	The CHNFs have superior antileaching property of clay with 3 times higher post-wetting clotting activity compared to QCG.					
30977359	7	60	theme	nm	1164:1165	arg1	diameter					1142:1149	their small average diameter	1122:1149	their small average diameter of 450 ± 260 nm	1122:1165	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	7	60	theme	nm	1164:1165	arg1	QCG					1114:1116	QCG	1114:1116	QCG	1114:1116	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	6	61	theme	thermal	850:856	arg1	properties					858:867	The physical and thermal properties	833:867	The physical and thermal properties of the CHNFs	833:880	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	3	62	theme	clotting	584:591	arg1	activity					593:600	3 times higher post-wetting clotting activity	556:600	3 times higher post-wetting clotting activity	556:600	The CHNFs have superior antileaching property of clay with 3 times higher post-wetting clotting activity compared to QCG.					
30977359	6	63	theme	X-ray	951:955	arg1	spectroscopy					957:968	energy-dispersive X-ray spectroscopy	933:968	energy-dispersive X-ray spectroscopy	933:968	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	9	64	theme	episodes	1706:1713	arg1	treatment					1673:1681	the treatment	1669:1681	the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders	1669:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	7	65	theme	±	1158:1158	arg1	nm					1164:1165	450 ± 260 nm	1154:1165	450 ± 260 nm	1154:1165	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	6	66	theme	physical	837:844	arg1	properties					858:867	The physical and thermal properties	833:867	The physical and thermal properties of the CHNFs	833:880	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	9	67	theme	coagulation	1467:1477	arg1	time					1479:1482	coagulation time	1467:1482	coagulation time	1467:1482	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	9	68	theme	patients	1718:1725	arg1	episodes					1706:1713	unexpected bleeding episodes	1686:1713	unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders	1686:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	4	69	theme	clotting	707:714	arg1	performance					716:726	twice the clotting performance	697:726	twice the clotting performance after washing	697:740	The CHNFs also coagulate whole blood 1.3 times faster than the QCG and retain twice the clotting performance after washing.					
30977359	2	70	theme	biocompatible	358:370	arg1	CHNFs					372:376	These novel biocompatible CHNFs	346:376	These novel biocompatible CHNFs	346:376	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	1	71	theme	wet-wet	229:235	arg1	process					253:259	a one-step wet-wet electrospinning process	218:259	a one-step wet-wet electrospinning process	218:259	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	6	72	theme	thermogravimetric	1040:1056	arg1	analysis					1058:1065	thermogravimetric analysis	1040:1065	thermogravimetric analysis	1040:1065	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	3	73	contain	have	507:510	arg2	property					534:541	superior antileaching property	512:541	superior antileaching property of clay	512:549	The CHNFs have superior antileaching property of clay with 3 times higher post-wetting clotting activity compared to QCG.					
30977359	3	73	contain	have	507:510	arg1	CHNFs					501:505	The CHNFs	497:505	The CHNFs	497:505	The CHNFs have superior antileaching property of clay with 3 times higher post-wetting clotting activity compared to QCG.					
30977359	7	74	theme	average	1134:1140	arg1	diameter					1142:1149	their small average diameter	1122:1149	their small average diameter of 450 ± 260 nm	1122:1165	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	5	75	from	coagulation	792:802	arg1	effective					772:780	effective	772:780	effective	772:780	Halloysite clay is also more effective in plasma coagulation than commercial kaolin clay.					
30977359	10	76	theme	second	1842:1847	arg1	minutes					1809:1815	minutes	1809:1815	minutes of heavy bleeding	1809:1833	Since a person can die within minutes of heavy bleeding, every second counts for stopping traumatic hemorrhaging.					
30977359	10	76	theme	second	1842:1847	arg1	counts					1849:1854	every second counts	1836:1854	every second counts for stopping traumatic hemorrhaging	1836:1890	Since a person can die within minutes of heavy bleeding, every second counts for stopping traumatic hemorrhaging.					
30977359	9	77	theme	novel	1495:1499	arg1	nanocomposite					1501:1513	this novel nanocomposite	1490:1513	this novel nanocomposite	1490:1513	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	9	78	theme	blood	1753:1757	arg1	disorders					1768:1776	hereditary blood clotting disorders	1742:1776	hereditary blood clotting disorders	1742:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	1	79	theme	cellulose-halloysite	136:155	arg1	CHNFs					190:194	CHNFs	190:194	CHNFs	190:194	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	1	79	theme	cellulose-halloysite	136:155	arg1	fibers					182:187	High-performance cellulose-halloysite hemostatic nanocomposite fibers	119:187	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs)	119:195	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	6	80	theme	area	1021:1024	arg1	analysis					1026:1033	Brunauer-Emmett-Teller surface area analysis	990:1033	Brunauer-Emmett-Teller surface area analysis	990:1033	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	10	81	theme	traumatic	1869:1877	arg1	hemorrhaging					1879:1890	traumatic hemorrhaging	1869:1890	traumatic hemorrhaging	1869:1890	Since a person can die within minutes of heavy bleeding, every second counts for stopping traumatic hemorrhaging.					
30977359	7	82	theme	clay	1096:1099	arg1	loading					1101:1107	a 7-fold greater clay loading	1079:1107	a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm	1079:1165	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	1	83	theme	nanocomposite	168:180	arg1	CHNFs					190:194	CHNFs	190:194	CHNFs	190:194	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	1	83	theme	nanocomposite	168:180	arg1	fibers					182:187	High-performance cellulose-halloysite hemostatic nanocomposite fibers	119:187	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs)	119:195	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
30977359	7	84	theme	±	1271:1271	arg1	μm					1277:1278	12.6 ± 0.9 μm	1266:1278	12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1	1266:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	9	85	dep	patients	1610:1617	arg1	undergoing					1619:1628	undergoing	1619:1628	undergoing major surgical procedures	1619:1654	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	9	85	dep	patients	1610:1617	arg1	aid					1662:1664	aid	1662:1664	to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders	1659:1776	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	0	86	theme	Plasma	94:99	arg1	Time					113:116	Human Plasma Coagulation Time	88:116	Human Plasma Coagulation Time	88:116	Novel Cellulose-Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time.					
30977359	7	87	theme	average	1246:1252	arg1	diameter					1254:1261	the larger average diameter	1235:1261	the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1	1235:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	9	88	theme	blood	1568:1572	arg1	loss					1574:1577	rapid blood loss	1562:1577	rapid blood loss	1562:1577	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	5	89	theme	plasma	785:790	arg1	coagulation					792:802	plasma coagulation	785:802	plasma coagulation	785:802	Halloysite clay is also more effective in plasma coagulation than commercial kaolin clay.					
30977359	9	90	theme	drastic	1446:1452	arg1	reduction					1454:1462	The drastic reduction	1442:1462	The drastic reduction in coagulation time	1442:1482	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	0	91	theme	Cellulose-Halloysite	6:25	arg1	Nanocomposite					38:50	Novel Cellulose-Halloysite Hemostatic Nanocomposite	0:50	Novel Cellulose-Halloysite Hemostatic Nanocomposite	0:50	Novel Cellulose-Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time.					
30977359	9	92	theme	military	1587:1594	arg1	personnel					1596:1604	military personnel	1587:1604	military personnel	1587:1604	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	7	93	theme	m2	1324:1325	arg1	g-1					1327:1329	1.6 m2 g-1	1320:1329	1.6 m2 g-1	1320:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	8	94	theme	primary	1382:1388	arg1	fibroblasts					1390:1400	human primary fibroblasts	1376:1400	human primary fibroblasts	1376:1400	The CHNFs were shown to be noncytotoxic and human primary fibroblasts proliferated on the composite material.					
30977359	3	95	theme	antileaching	521:532	arg1	property					534:541	superior antileaching property	512:541	superior antileaching property of clay	512:549	The CHNFs have superior antileaching property of clay with 3 times higher post-wetting clotting activity compared to QCG.					
30977359	2	96	theme	QuikClot	468:475	arg1	QCG					491:493	QCG	491:493	QCG	491:493	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	2	96	theme	QuikClot	468:475	arg1	Gauze					484:488	the industry gold standard QuikClot Combat Gauze	441:488	the industry gold standard QuikClot Combat Gauze (QCG)	441:494	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	0	97	theme	Dramatic	66:73	arg1	Reduction					75:83	a Dramatic Reduction	64:83	a Dramatic Reduction in Human Plasma Coagulation Time	64:116	Novel Cellulose-Halloysite Hemostatic Nanocomposite Fibers with a Dramatic Reduction in Human Plasma Coagulation Time.					
30977359	6	98	theme	scanning	903:910	arg1	microscopy					921:930	scanning electron microscopy	903:930	scanning electron microscopy	903:930	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	9	99	theme	surgical	1636:1643	arg1	procedures					1645:1654	major surgical procedures	1630:1654	major surgical procedures	1630:1654	The drastic reduction in coagulation time makes this novel nanocomposite a potential lifesaving material for victims of rapid blood loss such as military personnel and patients undergoing major surgical procedures or to aid in the treatment of unexpected bleeding episodes of patients suffering from hereditary blood clotting disorders.					
30977359	7	100	theme	surface	1304:1310	arg1	area					1312:1315	a specific surface area	1293:1315	a specific surface area of 1.6 m2 g-1	1293:1329	CHNFs show a 7-fold greater clay loading than QCG and their small average diameter of 450 ± 260 nm affords a greater specific surface area (33.6 m2 g-1) compared with the larger average diameter of 12.6 ± 0.9 μm for QCG with a specific surface area of 1.6 m2 g-1.					
30977359	2	101	theme	faster	396:401	arg1	time					422:425	2.4 times faster plasma coagulation time	386:425	2.4 times faster plasma coagulation time	386:425	These novel biocompatible CHNFs exhibit 2.4 times faster plasma coagulation time compared with the industry gold standard QuikClot Combat Gauze (QCG).					
30977359	6	102	theme	CHNFs	876:880	arg1	properties					858:867	The physical and thermal properties	833:867	The physical and thermal properties of the CHNFs	833:880	The physical and thermal properties of the CHNFs were evaluated using scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Brunauer-Emmett-Teller surface area analysis, and thermogravimetric analysis.					
30977359	3	103	theme	post-wetting	571:582	arg1	activity					593:600	3 times higher post-wetting clotting activity	556:600	3 times higher post-wetting clotting activity	556:600	The CHNFs have superior antileaching property of clay with 3 times higher post-wetting clotting activity compared to QCG.					
30977359	1	104	theme	plasma	285:290	arg1	coagulation					292:302	human plasma coagulation	279:302	human plasma coagulation	279:302	High-performance cellulose-halloysite hemostatic nanocomposite fibers (CHNFs) are fabricated using a one-step wet-wet electrospinning process and evaluated for human plasma coagulation by activated partial thromboplastin time.					
31413350	1	0	theme	microbiota-gut-brain	239:258	arg1	axis					260:263	the microbiota-gut-brain axis	235:263	the microbiota-gut-brain axis	235:263	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	4	1	theme	amyloid-β	1186:1194	arg1	accumulation					1196:1207	amyloid-β accumulation	1186:1207	amyloid-β accumulation	1186:1207	Furthermore, NK46 treatment suppressed amyloid-β, β/γ-secretases, and caspase-3 expression and amyloid-β accumulation in the hippocampus of 5XFAD-Tg mice.					
31413350	2	2	theme	microbiota	788:797	arg1	composition					799:809	gut microbiota composition	784:809	gut microbiota composition	784:809	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	2	2	theme	microbiota	788:797	arg1	Proteobacteria					825:838	Proteobacteria	825:838	Proteobacteria	825:838	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	2	3	theme	blood	859:863	arg1	levels					869:874	fecal and blood LPS levels	849:874	levels	869:874	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	5	4	theme	NF-κB	1347:1351	arg1	activation					1353:1362	NF-κB activation	1347:1362	NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice	1347:1407	NK46 treatment also reduced Iba1+, LPS+/CD11b+, and caspase-3+/NeuN+ cell populations and suppressed NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice, while BDNF expression was increased.					
31413350	5	5	theme	mice	1404:1407	arg1	hippocampus					1371:1381	the hippocampus	1367:1381	the hippocampus of 5XFAD-Tg and aged mice	1367:1407	NK46 treatment also reduced Iba1+, LPS+/CD11b+, and caspase-3+/NeuN+ cell populations and suppressed NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice, while BDNF expression was increased.					
31413350	3	6	theme	NK46	1015:1018	arg1	treatment					1020:1028	NK46 treatment	1015:1028	NK46 treatment	1015:1028	NK46 treatment also alleviated cognitive decline in 5XFAD-Tg and aged mice.					
31413350	1	7	theme	BV-2	485:488	arg1	cells					490:494	lipopolysaccharide (LPS)-stimulated BV-2 cells	449:494	lipopolysaccharide (LPS)-stimulated BV-2 cells	449:494	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	6	8	theme	production	1516:1525	arg1	suppression					1479:1489	the suppression	1475:1489	the suppression of gut dysbiosis and LPS production by NK46	1475:1533	These findings suggest that the suppression of gut dysbiosis and LPS production by NK46 can mitigate cognitive decline through the regulation of microbiota LPS-mediated NF-κB activation.					
31413350	1	9	theme	gut	378:380	arg1	production					403:412	gut microbiota endotoxin production	378:412	gut microbiota endotoxin production	378:412	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	1	10	theme	cognitive	190:198	arg1	decline					200:206	cognitive decline	190:206	cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis	190:263	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	2	11	theme	5XFAD-Tg	991:998	arg1	mice					1009:1012	5XFAD-Tg and aged mice	991:1012	5XFAD-Tg and aged mice	991:1012	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	1	12	theme	endotoxin	393:401	arg1	production					403:412	gut microbiota endotoxin production	378:412	gut microbiota endotoxin production	378:412	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	1	13	from	decline	200:206	arg1	disease					223:229	Alzheimer's disease	211:229	Alzheimer's disease	211:229	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	1	14	from	decline	582:588	arg1	mice					665:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	1	15	theme	NF-κB	429:433	arg1	activation					435:444	NF-κB activation	429:444	NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells	429:494	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	2	16	theme	aged	1004:1007	arg1	mice					1009:1012	5XFAD-Tg and aged mice	991:1012	5XFAD-Tg and aged mice	991:1012	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	1	17	from	activation	435:444	arg1	cells					490:494	lipopolysaccharide (LPS)-stimulated BV-2 cells	449:494	lipopolysaccharide (LPS)-stimulated BV-2 cells	449:494	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	2	18	theme	tight	941:945	arg1	expression					964:973	tight junction protein expression	941:973	tight junction protein expression in the colon of 5XFAD-Tg and aged mice	941:1012	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	5	19	theme	NK46	1246:1249	arg1	treatment					1251:1259	NK46 treatment	1246:1259	NK46 treatment	1246:1259	NK46 treatment also reduced Iba1+, LPS+/CD11b+, and caspase-3+/NeuN+ cell populations and suppressed NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice, while BDNF expression was increased.					
31413350	1	20	theme	gut	337:339	arg1	microbiota					341:350	human gut microbiota	331:350	human gut microbiota	331:350	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	3	21	theme	cognitive	1046:1054	arg1	decline					1056:1062	cognitive decline	1046:1062	cognitive decline in 5XFAD-Tg and aged mice	1046:1088	NK46 treatment also alleviated cognitive decline in 5XFAD-Tg and aged mice.					
31413350	1	22	theme	gut	155:157	arg1	bacteria					159:166	commensal gut bacteria	145:166	commensal gut bacteria	145:166	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	1	23	theme	aged	644:647	arg1	mice					665:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	0	24	theme	transgenic	93:102	arg1	mice					113:116	5XFAD transgenic and aged mice	87:116	5XFAD transgenic and aged mice	87:116	Suppression of gut dysbiosis by Bifidobacterium longum alleviates cognitive decline in 5XFAD transgenic and aged mice.					
31413350	1	25	from	role	137:140	arg1	progression					175:185	the progression	171:185	the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis	171:263	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	5	26	theme	caspase-3+/NeuN+	1298:1313	arg1	populations					1320:1330	caspase-3+/NeuN+ cell populations	1298:1330	caspase-3+/NeuN+ cell populations	1298:1330	NK46 treatment also reduced Iba1+, LPS+/CD11b+, and caspase-3+/NeuN+ cell populations and suppressed NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice, while BDNF expression was increased.					
31413350	4	27	theme	NK46	1104:1107	arg1	treatment					1109:1117	NK46 treatment	1104:1117	NK46 treatment	1104:1117	Furthermore, NK46 treatment suppressed amyloid-β, β/γ-secretases, and caspase-3 expression and amyloid-β accumulation in the hippocampus of 5XFAD-Tg mice.					
31413350	4	28	theme	5XFAD-Tg	1231:1238	arg1	mice					1240:1243	5XFAD-Tg mice	1231:1243	5XFAD-Tg mice	1231:1243	Furthermore, NK46 treatment suppressed amyloid-β, β/γ-secretases, and caspase-3 expression and amyloid-β accumulation in the hippocampus of 5XFAD-Tg mice.					
31413350	0	29	theme	aged	108:111	arg1	mice					113:116	5XFAD transgenic and aged mice	87:116	5XFAD transgenic and aged mice	87:116	Suppression of gut dysbiosis by Bifidobacterium longum alleviates cognitive decline in 5XFAD transgenic and aged mice.					
31413350	2	30	theme	protein	956:962	arg1	expression					964:973	tight junction protein expression	941:973	tight junction protein expression in the colon of 5XFAD-Tg and aged mice	941:1012	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	2	31	theme	Oral	671:674	arg1	administration					676:689	Oral administration	671:689	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively)	671:774	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	1	32	theme	cognitive	572:580	arg1	decline					582:588	cognitive decline	572:588	cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	572:668	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	6	33	theme	activation	1622:1631	arg1	regulation					1578:1587	the regulation	1574:1587	the regulation of microbiota LPS-mediated NF-κB activation	1574:1631	These findings suggest that the suppression of gut dysbiosis and LPS production by NK46 can mitigate cognitive decline through the regulation of microbiota LPS-mediated NF-κB activation.					
31413350	2	34	dep	NK46	694:697	arg1	CFU/mouse/day					708:720	1 × 109 CFU/mouse/day	700:720	1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice	700:759	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	2	35	theme	NF-κB	888:892	arg1	activation					894:903	NF-κB activation	888:903	NF-κB activation	888:903	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	0	36	theme	gut	15:17	arg1	dysbiosis					19:27	gut dysbiosis	15:27	gut dysbiosis	15:27	Suppression of gut dysbiosis by Bifidobacterium longum alleviates cognitive decline in 5XFAD transgenic and aged mice.					
31413350	1	37	theme	5xFAD-transgenic	598:613	arg1	mice					665:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	2	38	from	expression	964:973	arg1	colon					982:986	the colon	978:986	the colon of 5XFAD-Tg and aged mice	978:1012	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	2	39	from	months	734:739	arg1	mice					756:759	aged and Tg mice	744:759	mice	756:759	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	5	40	theme	cell	1315:1318	arg1	populations					1320:1330	caspase-3+/NeuN+ cell populations	1298:1330	caspase-3+/NeuN+ cell populations	1298:1330	NK46 treatment also reduced Iba1+, LPS+/CD11b+, and caspase-3+/NeuN+ cell populations and suppressed NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice, while BDNF expression was increased.					
31413350	4	41	from	expression	1171:1180	arg1	hippocampus					1216:1226	the hippocampus	1212:1226	the hippocampus of 5XFAD-Tg mice	1212:1243	Furthermore, NK46 treatment suppressed amyloid-β, β/γ-secretases, and caspase-3 expression and amyloid-β accumulation in the hippocampus of 5XFAD-Tg mice.					
31413350	0	42	theme	cognitive	66:74	arg1	decline					76:82	cognitive decline	66:82	cognitive decline in 5XFAD transgenic and aged mice	66:116	Suppression of gut dysbiosis by Bifidobacterium longum alleviates cognitive decline in 5XFAD transgenic and aged mice.					
31413350	5	43	theme	5XFAD-Tg	1386:1393	arg1	mice					1404:1407	5XFAD-Tg and aged mice	1386:1407	5XFAD-Tg and aged mice	1386:1407	NK46 treatment also reduced Iba1+, LPS+/CD11b+, and caspase-3+/NeuN+ cell populations and suppressed NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice, while BDNF expression was increased.					
31413350	1	44	dep	anti-inflammatory	278:294	arg1	NK46					320:323	NK46	320:323	NK46	320:323	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	1	44	dep	anti-inflammatory	278:294	arg1	longum					312:317	Bifidobacterium longum	296:317	Bifidobacterium longum (NK46)	296:324	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	2	45	theme	gut	784:786	arg1	composition					799:809	gut microbiota composition	784:809	gut microbiota composition	784:809	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	2	45	theme	gut	784:786	arg1	Proteobacteria					825:838	Proteobacteria	825:838	Proteobacteria	825:838	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	5	46	theme	aged	1399:1402	arg1	mice					1404:1407	5XFAD-Tg and aged mice	1386:1407	5XFAD-Tg and aged mice	1386:1407	NK46 treatment also reduced Iba1+, LPS+/CD11b+, and caspase-3+/NeuN+ cell populations and suppressed NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice, while BDNF expression was increased.					
31413350	4	47	from	β/γ-secretases	1141:1154	arg1	hippocampus					1216:1226	the hippocampus	1212:1226	the hippocampus of 5XFAD-Tg mice	1212:1243	Furthermore, NK46 treatment suppressed amyloid-β, β/γ-secretases, and caspase-3 expression and amyloid-β accumulation in the hippocampus of 5XFAD-Tg mice.					
31413350	4	48	from	amyloid-β	1130:1138	arg1	hippocampus					1216:1226	the hippocampus	1212:1226	the hippocampus of 5XFAD-Tg mice	1212:1243	Furthermore, NK46 treatment suppressed amyloid-β, β/γ-secretases, and caspase-3 expression and amyloid-β accumulation in the hippocampus of 5XFAD-Tg mice.					
31413350	2	49	theme	LPS	865:867	arg1	levels					869:874	fecal and blood LPS levels	849:874	levels	869:874	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	2	50	theme	aged	744:747	arg1	mice					756:759	aged and Tg mice	744:759	mice	756:759	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	1	51	theme	bacteria	159:166	arg1	role					137:140	the role	133:140	the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis	133:263	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	1	52	theme	-stimulated	473:483	arg1	cells					490:494	lipopolysaccharide (LPS)-stimulated BV-2 cells	449:494	lipopolysaccharide (LPS)-stimulated BV-2 cells	449:494	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	3	53	from	decline	1056:1062	arg1	mice					1085:1088	5XFAD-Tg and aged mice	1067:1088	5XFAD-Tg and aged mice	1067:1088	NK46 treatment also alleviated cognitive decline in 5XFAD-Tg and aged mice.					
31413350	5	54	theme	BDNF	1416:1419	arg1	expression					1421:1430	BDNF expression	1416:1430	BDNF expression	1416:1430	NK46 treatment also reduced Iba1+, LPS+/CD11b+, and caspase-3+/NeuN+ cell populations and suppressed NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice, while BDNF expression was increased.					
31413350	6	55	theme	LPS	1512:1514	arg1	production					1516:1525	LPS production	1512:1525	LPS production	1512:1525	These findings suggest that the suppression of gut dysbiosis and LPS production by NK46 can mitigate cognitive decline through the regulation of microbiota LPS-mediated NF-κB activation.					
31413350	2	56	theme	Tg	753:754	arg1	mice					756:759	aged and Tg mice	744:759	mice	756:759	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	1	57	theme	microbiota	382:391	arg1	production					403:412	gut microbiota endotoxin production	378:412	gut microbiota endotoxin production	378:412	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	6	58	theme	dysbiosis	1498:1506	arg1	suppression					1479:1489	the suppression	1475:1489	the suppression of gut dysbiosis and LPS production by NK46	1475:1533	These findings suggest that the suppression of gut dysbiosis and LPS production by NK46 can mitigate cognitive decline through the regulation of microbiota LPS-mediated NF-κB activation.					
31413350	1	59	theme	decline	200:206	arg1	progression					175:185	the progression	171:185	the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis	171:263	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	0	60	from	decline	76:82	arg1	mice					113:116	5XFAD transgenic and aged mice	87:116	5XFAD transgenic and aged mice	87:116	Suppression of gut dysbiosis by Bifidobacterium longum alleviates cognitive decline in 5XFAD transgenic and aged mice.					
31413350	4	61	theme	caspase-3	1161:1169	arg1	expression					1171:1180	caspase-3 expression	1161:1180	caspase-3 expression	1161:1180	Furthermore, NK46 treatment suppressed amyloid-β, β/γ-secretases, and caspase-3 expression and amyloid-β accumulation in the hippocampus of 5XFAD-Tg mice.					
31413350	1	62	from	disease	223:229	arg1	progression					175:185	the progression	171:185	the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis	171:263	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	6	63	theme	microbiota	1592:1601	arg1	activation					1622:1631	microbiota LPS-mediated NF-κB activation	1592:1631	microbiota LPS-mediated NF-κB activation	1592:1631	These findings suggest that the suppression of gut dysbiosis and LPS production by NK46 can mitigate cognitive decline through the regulation of microbiota LPS-mediated NF-κB activation.					
31413350	2	64	theme	NK46	694:697	arg1	administration					676:689	Oral administration	671:689	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively)	671:774	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	2	65	theme	mice	1009:1012	arg1	colon					982:986	the colon	978:986	the colon of 5XFAD-Tg and aged mice	978:1012	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	1	66	from	progression	175:185	arg1	disease					223:229	Alzheimer's disease	211:229	Alzheimer's disease	211:229	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	2	67	theme	junction	947:954	arg1	expression					964:973	tight junction protein expression	941:973	tight junction protein expression in the colon of 5XFAD-Tg and aged mice	941:1012	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	1	68	from	dysbiosis	558:566	arg1	mice					665:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	0	69	theme	5XFAD	87:91	arg1	mice					113:116	5XFAD transgenic and aged mice	87:116	5XFAD transgenic and aged mice	87:116	Suppression of gut dysbiosis by Bifidobacterium longum alleviates cognitive decline in 5XFAD transgenic and aged mice.					
31413350	1	70	theme	human	331:335	arg1	microbiota					341:350	human gut microbiota	331:350	human gut microbiota	331:350	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	6	71	theme	cognitive	1548:1556	arg1	decline					1558:1564	cognitive decline	1548:1564	cognitive decline	1548:1564	These findings suggest that the suppression of gut dysbiosis and LPS production by NK46 can mitigate cognitive decline through the regulation of microbiota LPS-mediated NF-κB activation.					
31413350	1	72	theme	commensal	145:153	arg1	bacteria					159:166	commensal gut bacteria	145:166	commensal gut bacteria	145:166	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	4	73	theme	mice	1240:1243	arg1	hippocampus					1216:1226	the hippocampus	1212:1226	the hippocampus of 5XFAD-Tg mice	1212:1243	Furthermore, NK46 treatment suppressed amyloid-β, β/γ-secretases, and caspase-3 expression and amyloid-β accumulation in the hippocampus of 5XFAD-Tg mice.					
31413350	1	74	theme	gut	554:556	arg1	dysbiosis					558:566	gut dysbiosis	554:566	gut dysbiosis	554:566	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	3	75	theme	5XFAD-Tg	1067:1074	arg1	mice					1085:1088	5XFAD-Tg and aged mice	1067:1088	5XFAD-Tg and aged mice	1067:1088	NK46 treatment also alleviated cognitive decline in 5XFAD-Tg and aged mice.					
31413350	5	76	from	activation	1353:1362	arg1	hippocampus					1371:1381	the hippocampus	1367:1381	the hippocampus of 5XFAD-Tg and aged mice	1367:1407	NK46 treatment also reduced Iba1+, LPS+/CD11b+, and caspase-3+/NeuN+ cell populations and suppressed NF-κB activation in the hippocampus of 5XFAD-Tg and aged mice, while BDNF expression was increased.					
31413350	0	77	theme	dysbiosis	19:27	arg1	Suppression					0:10	Suppression	0:10	Suppression of gut dysbiosis by Bifidobacterium longum	0:53	Suppression of gut dysbiosis by Bifidobacterium longum alleviates cognitive decline in 5XFAD transgenic and aged mice.					
31413350	6	78	theme	NF-κB	1616:1620	arg1	activation					1622:1631	microbiota LPS-mediated NF-κB activation	1592:1631	microbiota LPS-mediated NF-κB activation	1592:1631	These findings suggest that the suppression of gut dysbiosis and LPS production by NK46 can mitigate cognitive decline through the regulation of microbiota LPS-mediated NF-κB activation.					
31413350	4	79	from	accumulation	1196:1207	arg1	hippocampus					1216:1226	the hippocampus	1212:1226	the hippocampus of 5XFAD-Tg mice	1212:1243	Furthermore, NK46 treatment suppressed amyloid-β, β/γ-secretases, and caspase-3 expression and amyloid-β accumulation in the hippocampus of 5XFAD-Tg mice.					
31413350	1	80	theme	male	593:596	arg1	mice					665:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice	593:668	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	3	81	theme	aged	1080:1083	arg1	mice					1085:1088	5XFAD-Tg and aged mice	1067:1088	5XFAD-Tg and aged mice	1067:1088	NK46 treatment also alleviated cognitive decline in 5XFAD-Tg and aged mice.					
31413350	2	82	theme	TNF-α	909:913	arg1	expression					915:924	TNF-α expression	909:924	TNF-α expression	909:924	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	6	83	theme	gut	1494:1496	arg1	dysbiosis					1498:1506	gut dysbiosis	1494:1506	gut dysbiosis	1494:1506	These findings suggest that the suppression of gut dysbiosis and LPS production by NK46 can mitigate cognitive decline through the regulation of microbiota LPS-mediated NF-κB activation.					
31413350	2	84	theme	fecal	849:853	arg1	levels					869:874	fecal and blood LPS levels	849:874	levels	869:874	Oral administration of NK46 (1 × 109 CFU/mouse/day for 1 and 2 months in aged and Tg mice, respectively) shifted gut microbiota composition, particularly Proteobacteria, reduced fecal and blood LPS levels, suppressed NF-κB activation and TNF-α expression, and increased tight junction protein expression in the colon of 5XFAD-Tg and aged mice.					
31413350	6	85	theme	LPS-mediated	1603:1614	arg1	activation					1622:1631	microbiota LPS-mediated NF-κB activation	1592:1631	microbiota LPS-mediated NF-κB activation	1592:1631	These findings suggest that the suppression of gut dysbiosis and LPS production by NK46 can mitigate cognitive decline through the regulation of microbiota LPS-mediated NF-κB activation.					
31413350	1	86	dep	5xFAD-transgenic	598:613	arg1	months-old					653:662	18 months-old	650:662	18 months-old	650:662	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	1	86	dep	5xFAD-transgenic	598:613	arg1	5XFAD-Tg					616:623	5XFAD-Tg	616:623	5XFAD-Tg	616:623	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31413350	1	86	dep	5xFAD-transgenic	598:613	arg1	months-old					628:637	6 months-old	626:637	6 months-old	626:637	To understand the role of commensal gut bacteria on the progression of cognitive decline in Alzheimer's disease via the microbiota-gut-brain axis, we isolated anti-inflammatory Bifidobacterium longum (NK46) from human gut microbiota, which potently inhibited gut microbiota endotoxin production and suppressed NF-κB activation in lipopolysaccharide (LPS)-stimulated BV-2 cells, and examined whether NK46 could simultaneously alleviate gut dysbiosis and cognitive decline in male 5xFAD-transgenic (5XFAD-Tg, 6 months-old) and aged (18 months-old) mice.					
31874264	6	0	theme	amorphous	869:877	arg1	halo					879:882	a dominant amorphous halo	858:882	a dominant amorphous halo	858:882	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	6	1	from	spherical	915:923	arg1	shape					928:932	shape	928:932	shape	928:932	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	6	2	theme	dominant	860:867	arg1	halo					879:882	a dominant amorphous halo	858:882	a dominant amorphous halo	858:882	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	9	3	theme	high	1196:1199	arg1	efficiency					1220:1229	high microencapsulation efficiency	1196:1229	high microencapsulation efficiency	1196:1229	Overall, the results led to the conclusion that tamarind mucilage is a viable material for high microencapsulation efficiency, while offering protection against oxidation mechanisms of SO.					
31874264	9	4	theme	viable	1176:1181	arg1	mucilage					1162:1169	tamarind mucilage	1153:1169	tamarind mucilage	1153:1169	Overall, the results led to the conclusion that tamarind mucilage is a viable material for high microencapsulation efficiency, while offering protection against oxidation mechanisms of SO.					
31874264	9	4	theme	viable	1176:1181	arg1	material					1183:1190	a viable material	1174:1190	a viable material for high microencapsulation efficiency	1174:1229	Overall, the results led to the conclusion that tamarind mucilage is a viable material for high microencapsulation efficiency, while offering protection against oxidation mechanisms of SO.					
31874264	5	5	theme	bimodal	665:671	arg1	distribution					673:684	a bimodal distribution	663:684	a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm	663:734	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	4	6	theme	size	534:537	arg1	monomodal					577:585	monomodal	577:585	monomodal	577:585	The particle size distribution for M1 microcapsules was monomodal with diameter in the range 1-50 μm.					
31874264	4	6	theme	size	534:537	arg1	distribution					539:550	The particle size distribution	521:550	The particle size distribution for M1 microcapsules	521:571	The particle size distribution for M1 microcapsules was monomodal with diameter in the range 1-50 μm.					
31874264	3	7	theme	M2	464:465	arg1	microcapsules					467:479	M1 and M2 microcapsules	457:479	M1 and M2 microcapsules	457:479	Powder of M1 and M2 microcapsules exhibited free-flowing characteristics.					
31874264	4	8	theme	particle	525:532	arg1	monomodal					577:585	monomodal	577:585	monomodal	577:585	The particle size distribution for M1 microcapsules was monomodal with diameter in the range 1-50 μm.					
31874264	4	8	theme	particle	525:532	arg1	distribution					539:550	The particle size distribution	521:550	The particle size distribution for M1 microcapsules	521:571	The particle size distribution for M1 microcapsules was monomodal with diameter in the range 1-50 μm.					
31874264	8	9	theme	Peroxide	997:1004	arg1	formation					1006:1014	Peroxide formation	997:1014	Peroxide formation	997:1014	Peroxide formation reached values after six weeks was 14.65 and 16.51 mEq/kgOil for M1 and M2 respectively.					
31874264	1	10	theme	Tamarind	65:72	arg1	material					124:131	a novel wall material	111:131	a novel wall material for microencapsulation of sesame oil (SO)	111:173	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	1	10	theme	Tamarind	65:72	arg1	TSM					89:91	TSM	89:91	TSM	89:91	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	1	10	theme	Tamarind	65:72	arg1	mucilage					79:86	Tamarind seed mucilage	65:86	Tamarind seed mucilage (TSM)	65:92	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	4	11	from	range	608:612	arg1	monomodal					577:585	monomodal	577:585	monomodal	577:585	The particle size distribution for M1 microcapsules was monomodal with diameter in the range 1-50 μm.					
31874264	4	11	from	range	608:612	arg1	distribution					539:550	The particle size distribution	521:550	The particle size distribution for M1 microcapsules	521:571	The particle size distribution for M1 microcapsules was monomodal with diameter in the range 1-50 μm.					
31874264	0	12	theme	tamarind	41:48	arg1	mucilage					55:62	tamarind seed mucilage	41:62	tamarind seed mucilage	41:62	Microencapsulation of sesame seed oil by tamarind seed mucilage.					
31874264	9	13	theme	oxidation	1266:1274	arg1	mechanisms					1276:1285	oxidation mechanisms	1266:1285	oxidation mechanisms of SO	1266:1291	Overall, the results led to the conclusion that tamarind mucilage is a viable material for high microencapsulation efficiency, while offering protection against oxidation mechanisms of SO.					
31874264	6	14	theme	M1	737:738	arg1	microcapsules					740:752	M1 microcapsules	737:752	M1 microcapsules	737:752	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	1	15	theme	seed	74:77	arg1	material					124:131	a novel wall material	111:131	a novel wall material for microencapsulation of sesame oil (SO)	111:173	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	1	15	theme	seed	74:77	arg1	TSM					89:91	TSM	89:91	TSM	89:91	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	1	15	theme	seed	74:77	arg1	mucilage					79:86	Tamarind seed mucilage	65:86	Tamarind seed mucilage (TSM)	65:92	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	2	16	theme	flow	302:305	arg1	properties					307:316	the corresponding physical and flow properties	271:316	properties	307:316	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	3	17	theme	free-flowing	491:502	arg1	characteristics					504:518	free-flowing characteristics	491:518	free-flowing characteristics	491:518	Powder of M1 and M2 microcapsules exhibited free-flowing characteristics.					
31874264	5	18	attach	presented	653:661	arg1	contrast					626:633	contrast	626:633	contrast	626:633	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	5	18	attach	presented	653:661	arg2	M2					650:651	Microcapsules M2	636:651	Microcapsules M2	636:651	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	1	19	theme	sesame	159:164	arg1	oil					166:168	sesame oil	159:168	sesame oil (SO)	159:173	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	6	20	dep	227 °C	782:787	arg1	M2					807:808	M2	807:808	M2	807:808	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	2	21	theme	1:2	241:243	arg1	ratios					218:223	core ratios	213:223	core ratios of 1:1 (M1) and 1:2 (M2)	213:248	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	1	22	theme	oil	166:168	arg1	microencapsulation					137:154	microencapsulation	137:154	microencapsulation of sesame oil (SO)	137:173	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	0	23	theme	seed	29:32	arg1	oil					34:36	sesame seed oil	22:36	sesame seed oil	22:36	Microencapsulation of sesame seed oil by tamarind seed mucilage.					
31874264	9	24	theme	SO	1290:1291	arg1	mechanisms					1276:1285	oxidation mechanisms	1266:1285	oxidation mechanisms of SO	1266:1291	Overall, the results led to the conclusion that tamarind mucilage is a viable material for high microencapsulation efficiency, while offering protection against oxidation mechanisms of SO.					
31874264	5	25	theme	Microcapsules	636:648	arg1	M2					650:651	Microcapsules M2	636:651	Microcapsules M2	636:651	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	9	26	theme	tamarind	1153:1160	arg1	mucilage					1162:1169	tamarind mucilage	1153:1169	tamarind mucilage	1153:1169	Overall, the results led to the conclusion that tamarind mucilage is a viable material for high microencapsulation efficiency, while offering protection against oxidation mechanisms of SO.					
31874264	9	26	theme	tamarind	1153:1160	arg1	material					1183:1190	a viable material	1174:1190	a viable material for high microencapsulation efficiency	1174:1229	Overall, the results led to the conclusion that tamarind mucilage is a viable material for high microencapsulation efficiency, while offering protection against oxidation mechanisms of SO.					
31874264	3	27	theme	microcapsules	467:479	arg1	Powder					447:452	Powder	447:452	Powder of M1 and M2 microcapsules	447:479	Powder of M1 and M2 microcapsules exhibited free-flowing characteristics.					
31874264	0	28	theme	sesame	22:27	arg1	oil					34:36	sesame seed oil	22:36	sesame seed oil	22:36	Microencapsulation of sesame seed oil by tamarind seed mucilage.					
31874264	2	29	theme	1:1	228:230	arg1	ratios					218:223	core ratios	213:223	core ratios of 1:1 (M1) and 1:2 (M2)	213:248	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	4	30	from	monomodal	577:585	arg1	range					608:612	the range 1-50 μm	604:620	the range 1-50 μm	604:620	The particle size distribution for M1 microcapsules was monomodal with diameter in the range 1-50 μm.					
31874264	6	31	from	shape	928:932	arg1	spherical					915:923	spherical	915:923	spherical	915:923	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	6	32	theme	spherical	915:923	arg1	halo					879:882	a dominant amorphous halo	858:882	a dominant amorphous halo	858:882	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	6	33	theme	178 °C.	816:822	arg1	M2					845:846	M2	845:846	M2	845:846	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	6	33	theme	178 °C.	816:822	arg1	M1					838:839	M1	838:839	M1	838:839	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	6	33	theme	178 °C.	816:822	arg1	Microcapsules					824:836	178 °C. Microcapsules	816:836	178 °C. Microcapsules M1 and M2	816:846	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	2	34	theme	Wall	199:202	arg1	material					204:211	Wall material	199:211	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.	199:445	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	2	35	theme	thermal	319:325	arg1	physical					289:296	physical	289:296	physical	289:296	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	2	35	theme	thermal	319:325	arg1	stability					327:335	thermal stability	319:335	thermal stability	319:335	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	0	36	theme	oil	34:36	arg1	Microencapsulation					0:17	Microencapsulation	0:17	Microencapsulation of sesame seed oil by tamarind seed mucilage	0:62	Microencapsulation of sesame seed oil by tamarind seed mucilage.					
31874264	2	37	theme	encapsulation	381:393	arg1	physical					289:296	physical	289:296	physical	289:296	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	2	37	theme	encapsulation	381:393	arg1	efficiency					395:404	encapsulation efficiency	381:404	encapsulation efficiency	381:404	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	1	38	dep	oil	166:168	arg1	SO					171:172	SO	171:172	SO	171:172	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	0	39	theme	seed	50:53	arg1	mucilage					55:62	tamarind seed mucilage	41:62	tamarind seed mucilage	41:62	Microencapsulation of sesame seed oil by tamarind seed mucilage.					
31874264	5	40	from	distribution	673:684	arg1	ranges					707:712	the ranges 1-50 μm and 50-125 μm	703:734	the ranges 1-50 μm and 50-125 μm	703:734	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	5	40	from	distribution	673:684	arg1	50-125 μm					726:734	50-125 μm	726:734	50-125 μm	726:734	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	5	40	from	distribution	673:684	arg1	1-50 μm					714:720	1-50 μm	714:720	1-50 μm	714:720	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	2	41	theme	groups	349:354	arg1	composition					356:366	functional groups composition	338:366	functional groups composition	338:366	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	2	41	theme	groups	349:354	arg1	physical					289:296	physical	289:296	physical	289:296	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	6	42	theme	external	888:895	arg1	morphology					897:906	external morphology	888:906	external morphology	888:906	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	5	43	dep	ranges	707:712	arg1	ranges					707:712	the ranges 1-50 μm and 50-125 μm	703:734	the ranges 1-50 μm and 50-125 μm	703:734	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	5	43	dep	ranges	707:712	arg1	50-125 μm					726:734	50-125 μm	726:734	50-125 μm	726:734	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	5	43	dep	ranges	707:712	arg1	1-50 μm					714:720	1-50 μm	714:720	1-50 μm	714:720	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	5	44	with	distribution	673:684	arg1	diameter					691:698	diameter	691:698	diameter	691:698	In contrast, Microcapsules M2 presented a bimodal distribution with diameter in the ranges 1-50 μm and 50-125 μm.					
31874264	7	45	theme	Encapsulation	935:947	arg1	efficiency					949:958	Encapsulation efficiency	935:958	Encapsulation efficiency	935:958	Encapsulation efficiency was 91.05% for M1 and 81.22% for M2.					
31874264	1	46	theme	spray-drying	178:189	arg1	method					191:196	spray-drying method	178:196	spray-drying method	178:196	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	3	47	theme	M1	457:458	arg1	microcapsules					467:479	M1 and M2 microcapsules	457:479	M1 and M2 microcapsules	457:479	Powder of M1 and M2 microcapsules exhibited free-flowing characteristics.					
31874264	4	48	with	monomodal	577:585	arg1	diameter					592:599	diameter	592:599	diameter	592:599	The particle size distribution for M1 microcapsules was monomodal with diameter in the range 1-50 μm.					
31874264	2	49	theme	core	213:216	arg1	ratios					218:223	core ratios	213:223	core ratios of 1:1 (M1) and 1:2 (M2)	213:248	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	9	50	theme	microencapsulation	1201:1218	arg1	efficiency					1220:1229	high microencapsulation efficiency	1196:1229	high microencapsulation efficiency	1196:1229	Overall, the results led to the conclusion that tamarind mucilage is a viable material for high microencapsulation efficiency, while offering protection against oxidation mechanisms of SO.					
31874264	1	51	theme	novel	113:117	arg1	material					124:131	a novel wall material	111:131	a novel wall material for microencapsulation of sesame oil (SO)	111:173	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	1	51	theme	novel	113:117	arg1	mucilage					79:86	Tamarind seed mucilage	65:86	Tamarind seed mucilage (TSM)	65:92	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	2	52	dep	material	204:211	arg1	evaluated					436:444	evaluated	436:444	were evaluated	431:444	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	2	52	dep	material	204:211	arg1	considered					255:264	considered	255:264	were considered	250:264	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	2	53	theme	oxidative	411:419	arg1	physical					289:296	physical	289:296	physical	289:296	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	2	53	theme	oxidative	411:419	arg1	stability					421:429	oxidative stability	411:429	oxidative stability	411:429	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	2	54	theme	functional	338:347	arg1	composition					356:366	functional groups composition	338:366	functional groups composition	338:366	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	2	54	theme	functional	338:347	arg1	physical					289:296	physical	289:296	physical	289:296	Wall material:core ratios of 1:1 (M1) and 1:2 (M2) were considered, and the corresponding physical and flow properties, thermal stability, functional groups composition, morphology, encapsulation efficiency, and oxidative stability were evaluated.					
31874264	6	55	dep	Microcapsules	824:836	arg1	M2					845:846	M2	845:846	M2	845:846	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	6	55	dep	Microcapsules	824:836	arg1	M1					838:839	M1	838:839	M1	838:839	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	6	55	dep	Microcapsules	824:836	arg1	Microcapsules					824:836	178 °C. Microcapsules	816:836	178 °C. Microcapsules M1 and M2	816:846	M1 microcapsules were thermally stable until 227 °C and microcapsules M2 until 178 °C. Microcapsules M1 and M2 exhibited a dominant amorphous halo and external morphology almost spherical in shape.					
31874264	1	56	theme	wall	119:122	arg1	material					124:131	a novel wall material	111:131	a novel wall material for microencapsulation of sesame oil (SO)	111:173	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	1	56	theme	wall	119:122	arg1	mucilage					79:86	Tamarind seed mucilage	65:86	Tamarind seed mucilage (TSM)	65:92	Tamarind seed mucilage (TSM) was evaluated as a novel wall material for microencapsulation of sesame oil (SO) by spray-drying method.					
31874264	4	57	theme	M1	556:557	arg1	microcapsules					559:571	M1 microcapsules	556:571	M1 microcapsules	556:571	The particle size distribution for M1 microcapsules was monomodal with diameter in the range 1-50 μm.					
30802890	7	0	dep	showed	1137:1142	arg1	changed					1187:1193	changed	1187:1193	showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold	1137:1344	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	2	1	theme	alginate	363:370	arg1	polymer					372:378	alginate polymer	363:378	alginate polymer	363:378	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	6	2	theme	atomic	934:939	arg1	microscopy					947:956	The scanning electron and atomic force microscopy	908:956	microscopy	947:956	The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation.					
30802890	3	3	theme	stem	605:608	arg1	MSC					617:619	MSC	617:619	MSC	617:619	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	3	3	theme	stem	605:608	arg1	cells					610:614	human mesenchymal stem cells	587:614	human mesenchymal stem cells (MSC)	587:620	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	7	4	theme	MSCs	1171:1174	arg1	morphology					1176:1185	MSCs morphology	1171:1185	MSCs morphology	1171:1185	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	5	5	theme	pure	845:848	arg1	NP					853:854	neither pure BT NP or BT NP/alginate 3D scaffold	837:884	NP	853:854	Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity.					
30802890	1	6	theme	promising	191:199	arg1	potential					207:215	promising great potential	191:215	promising great potential for scaffold composition in tissue engineering	191:262	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	1	6	theme	promising	191:199	arg1	molecules					180:188	extracellular matrix molecules	159:188	extracellular matrix molecules	159:188	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	9	7	theme	BT	1522:1523	arg1	scaffold					1540:1547	the BT NP/alginate 3D scaffold	1518:1547	the BT NP/alginate 3D scaffold	1518:1547	These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.					
30802890	4	8	theme	oxidative	666:674	arg1	stress					676:681	oxidative stress	666:681	oxidative stress	666:681	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	2	9	theme	titanate	318:325	arg1	nanoparticles					327:339	barium titanate nanoparticles	311:339	barium titanate nanoparticles (BT NP) combined with alginate polymer	311:378	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	2	9	theme	titanate	318:325	arg1	BT					342:343	BT NP	342:346	BT NP	342:346	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	5	10	theme	BT	859:860	arg1	scaffold					877:884	neither pure BT NP or BT NP/alginate 3D scaffold	837:884	scaffold	877:884	Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity.					
30802890	7	11	theme	Kossa	1122:1126	arg1	staining					1128:1135	Von Kossa staining	1118:1135	Von Kossa staining	1118:1135	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	1	12	from	composition	230:240	arg1	engineering					252:262	tissue engineering	245:262	tissue engineering	245:262	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	5	13	theme	3D	874:875	arg1	scaffold					877:884	neither pure BT NP or BT NP/alginate 3D scaffold	837:884	scaffold	877:884	Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity.					
30802890	6	14	theme	NP/alginate	975:985	arg1	scaffold					990:997	BT NP/alginate 3D scaffold	972:997	BT NP/alginate 3D scaffold produced	972:1006	The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation.					
30802890	4	15	theme	tetrazolium	763:773	arg1	MTT					784:786	MTT	784:786	MTT	784:786	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	4	15	theme	tetrazolium	763:773	arg1	bromide					775:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide	686:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT)	686:787	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	9	16	theme	cytocompatible	1560:1573	arg1	microenvironment					1589:1604	a cytocompatible and bioactive microenvironment	1558:1604	a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation	1558:1645	These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.					
30802890	4	17	theme	-2,5-diphenyltetrazolium	714:737	arg1	MTT					784:786	MTT	784:786	MTT	784:786	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	4	17	theme	-2,5-diphenyltetrazolium	714:737	arg1	bromide					775:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide	686:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT)	686:787	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	3	18	theme	mesenchymal	593:603	arg1	MSC					617:619	MSC	617:619	MSC	617:619	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	3	18	theme	mesenchymal	593:603	arg1	cells					610:614	human mesenchymal stem cells	587:614	human mesenchymal stem cells (MSC)	587:620	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	9	19	theme	bioactive	1579:1587	arg1	microenvironment					1589:1604	a cytocompatible and bioactive microenvironment	1558:1604	a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation	1558:1645	These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.					
30802890	8	20	theme	NP/alginate	1360:1370	arg1	scaffold					1375:1382	the BT NP/alginate 3D scaffold	1353:1382	the BT NP/alginate 3D scaffold	1353:1382	Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements.					
30802890	1	21	theme	extracellular	159:171	arg1	potential					207:215	promising great potential	191:215	promising great potential for scaffold composition in tissue engineering	191:262	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	1	21	theme	extracellular	159:171	arg1	molecules					180:188	extracellular matrix molecules	159:188	extracellular matrix molecules	159:188	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	4	22	theme	thiazolyl	748:756	arg1	MTT					784:786	MTT	784:786	MTT	784:786	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	4	22	theme	thiazolyl	748:756	arg1	bromide					775:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide	686:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT)	686:787	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	0	23	theme	osteogenic	77:86	arg1	differentiation					104:118	osteogenic human stem cell differentiation	77:118	osteogenic human stem cell differentiation	77:118	In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.					
30802890	1	24	theme	molecules	180:188	arg1	properties					145:154	properties	145:154	properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering	145:262	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	0	25	theme	stem	94:97	arg1	differentiation					104:118	osteogenic human stem cell differentiation	77:118	osteogenic human stem cell differentiation	77:118	In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.					
30802890	6	26	theme	interconnected	1025:1038	arg1	pores					1040:1044	highly interconnected pores	1018:1044	highly interconnected pores	1018:1044	The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation.					
30802890	8	27	theme	differentiation	1405:1419	arg1	potential					1431:1439	an osteogenic differentiation induction potential	1391:1439	an osteogenic differentiation induction potential	1391:1439	Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements.					
30802890	2	28	theme	three-dimensional	415:431	arg1	scaffold					438:445	a new cytocompatible three-dimensional (3D) scaffold	394:445	a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation	394:492	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	2	29	theme	stem	468:471	arg1	differentiation					478:492	osteogenic stem cell differentiation	457:492	osteogenic stem cell differentiation	457:492	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	7	30	dep	shape	1218:1222	arg1	spindle					1200:1206	spindle	1200:1206	spindle	1200:1206	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	2	31	theme	new	396:398	arg1	scaffold					438:445	a new cytocompatible three-dimensional (3D) scaffold	394:445	a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation	394:492	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	0	32	theme	In	0:1	arg1	evaluation					9:18	In vitro evaluation	0:18	In vitro evaluation of barium	0:28	In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.					
30802890	4	33	theme	superoxide	793:802	arg1	SOD					815:817	SOD	815:817	SOD	815:817	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	4	33	theme	superoxide	793:802	arg1	dismutase					804:812	superoxide dismutase	793:812	superoxide dismutase (SOD)	793:818	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	4	34	theme	bromide	739:745	arg1	MTT					784:786	MTT	784:786	MTT	784:786	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	4	34	theme	bromide	739:745	arg1	bromide					775:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide	686:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT)	686:787	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	0	35	theme	barium	23:28	arg1	evaluation					9:18	In vitro evaluation	0:18	In vitro evaluation of barium	0:28	In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.					
30802890	8	36	theme	osteogenic	1466:1475	arg1	supplements					1477:1487	osteogenic supplements	1466:1487	osteogenic supplements	1466:1487	Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements.					
30802890	0	37	theme	nanoparticle/alginate	39:59	arg1	scaffold					64:71	nanoparticle/alginate 3D scaffold	39:71	nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation	39:118	In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.					
30802890	1	38	theme	tissue	245:250	arg1	engineering					252:262	tissue engineering	245:262	tissue engineering	245:262	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	3	39	theme	human	587:591	arg1	MSC					617:619	MSC	617:619	MSC	617:619	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	3	39	theme	human	587:591	arg1	cells					610:614	human mesenchymal stem cells	587:614	human mesenchymal stem cells (MSC)	587:620	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	7	40	theme	NP/alginate	1322:1332	arg1	scaffold					1337:1344	the BT NP/alginate 3D scaffold	1315:1344	the BT NP/alginate 3D scaffold	1315:1344	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	2	41	dep	three-dimensional	415:431	arg1	3D					434:435	3D	434:435	3D	434:435	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	3	42	dep	In	495:496	arg1	vitro					498:502	vitro	498:502	vitro	498:502	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	4	43	theme	cell	647:650	arg1	viability					652:660	the cell viability	643:660	the cell viability	643:660	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	3	44	theme	differentiation	537:551	arg1	potential					553:561	osteogenic differentiation potential	526:561	osteogenic differentiation potential	526:561	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	6	45	theme	force	941:945	arg1	microscopy					947:956	The scanning electron and atomic force microscopy	908:956	microscopy	947:956	The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation.					
30802890	2	46	theme	barium	311:316	arg1	nanoparticles					327:339	barium titanate nanoparticles	311:339	barium titanate nanoparticles (BT NP) combined with alginate polymer	311:378	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	2	46	theme	barium	311:316	arg1	BT					342:343	BT NP	342:346	BT NP	342:346	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	9	47	theme	osteogenic	1610:1619	arg1	differentiation					1631:1645	osteogenic human MSC differentiation	1610:1645	osteogenic human MSC differentiation	1610:1645	These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.					
30802890	7	48	theme	cuboid	1211:1216	arg1	shape					1218:1222	cuboid shape	1211:1222	cuboid shape	1211:1222	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	6	49	theme	scanning	912:919	arg1	electron					921:928	The scanning electron and atomic force microscopy	908:956	electron	921:928	The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation.					
30802890	7	50	theme	mineralization	1144:1157	arg1	nodules					1159:1165	mineralization nodules	1144:1165	mineralization nodules	1144:1165	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	1	51	theme	great	201:205	arg1	potential					207:215	promising great potential	191:215	promising great potential for scaffold composition in tissue engineering	191:262	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	1	51	theme	great	201:205	arg1	molecules					180:188	extracellular matrix molecules	159:188	extracellular matrix molecules	159:188	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	9	52	theme	3D	1537:1538	arg1	scaffold					1540:1547	the BT NP/alginate 3D scaffold	1518:1547	the BT NP/alginate 3D scaffold	1518:1547	These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.					
30802890	9	53	theme	human	1621:1625	arg1	differentiation					1631:1645	osteogenic human MSC differentiation	1610:1645	osteogenic human MSC differentiation	1610:1645	These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.					
30802890	7	54	theme	Von	1118:1120	arg1	staining					1128:1135	Von Kossa staining	1118:1135	Von Kossa staining	1118:1135	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	5	55	theme	NP/alginate	862:872	arg1	scaffold					877:884	neither pure BT NP or BT NP/alginate 3D scaffold	837:884	scaffold	877:884	Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity.					
30802890	6	56	theme	3D	987:988	arg1	scaffold					990:997	BT NP/alginate 3D scaffold	972:997	BT NP/alginate 3D scaffold produced	972:1006	The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation.					
30802890	7	57	theme	ALP	1255:1257	arg1	mRNA					1259:1262	ALP mRNA	1255:1262	ALP mRNA	1255:1262	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	6	58	theme	BT	972:973	arg1	scaffold					990:997	BT NP/alginate 3D scaffold	972:997	BT NP/alginate 3D scaffold produced	972:1006	The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation.					
30802890	4	59	theme	blue	758:761	arg1	MTT					784:786	MTT	784:786	MTT	784:786	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	4	59	theme	blue	758:761	arg1	bromide					775:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide	686:781	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT)	686:787	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	4	60	theme	bromide	775:781	arg1	assays					820:825	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays	686:825	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays	686:825	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	2	61	theme	present	272:278	arg1	study					280:284	the present study	268:284	the present study	268:284	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	9	62	theme	NP/alginate	1525:1535	arg1	scaffold					1540:1547	the BT NP/alginate 3D scaffold	1518:1547	the BT NP/alginate 3D scaffold	1518:1547	These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.					
30802890	8	63	theme	BT	1357:1358	arg1	scaffold					1375:1382	the BT NP/alginate 3D scaffold	1353:1382	the BT NP/alginate 3D scaffold	1353:1382	Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements.					
30802890	0	64	theme	human	88:92	arg1	differentiation					104:118	osteogenic human stem cell differentiation	77:118	osteogenic human stem cell differentiation	77:118	In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.					
30802890	1	65	theme	matrix	173:178	arg1	potential					207:215	promising great potential	191:215	promising great potential for scaffold composition in tissue engineering	191:262	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	1	65	theme	matrix	173:178	arg1	molecules					180:188	extracellular matrix molecules	159:188	extracellular matrix molecules	159:188	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	6	66	theme	surface	1050:1056	arg1	nanotopography					1058:1071	surface nanotopography	1050:1071	surface nanotopography	1050:1071	The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation.					
30802890	7	67	dep	d.	1233:1234	arg1	upregulated					1283:1293	upregulated	1283:1293	were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold	1264:1344	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	8	68	theme	3D	1372:1373	arg1	scaffold					1375:1382	the BT NP/alginate 3D scaffold	1353:1382	the BT NP/alginate 3D scaffold	1353:1382	Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements.					
30802890	0	69	theme	cell	99:102	arg1	differentiation					104:118	osteogenic human stem cell differentiation	77:118	osteogenic human stem cell differentiation	77:118	In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.					
30802890	9	70	theme	MSC	1627:1629	arg1	differentiation					1631:1645	osteogenic human MSC differentiation	1610:1645	osteogenic human MSC differentiation	1610:1645	These results indicate that the BT NP/alginate 3D scaffold provides a cytocompatible and bioactive microenvironment for osteogenic human MSC differentiation.					
30802890	4	71	theme	dismutase	804:812	arg1	assays					820:825	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays	686:825	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays	686:825	Firstly, we studied the cell viability and oxidative stress by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) thiazolyl blue tetrazolium bromide (MTT) and superoxide dismutase (SOD) assays.					
30802890	1	72	theme	scaffold	221:228	arg1	composition					230:240	scaffold composition	221:240	scaffold composition in tissue engineering	221:262	Nanomaterials can mimic properties of extracellular matrix molecules, promising great potential for scaffold composition in tissue engineering.					
30802890	8	73	theme	osteogenic	1394:1403	arg1	potential					1431:1439	an osteogenic differentiation induction potential	1391:1439	an osteogenic differentiation induction potential	1391:1439	Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements.					
30802890	2	74	theme	osteogenic	457:466	arg1	differentiation					478:492	osteogenic stem cell differentiation	457:492	osteogenic stem cell differentiation	457:492	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	8	75	theme	induction	1421:1429	arg1	potential					1431:1439	an osteogenic differentiation induction potential	1391:1439	an osteogenic differentiation induction potential	1391:1439	Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements.					
30802890	5	76	theme	BT	850:851	arg1	NP					853:854	neither pure BT NP or BT NP/alginate 3D scaffold	837:884	NP	853:854	Overall, neither pure BT NP or BT NP/alginate 3D scaffold induced cytotoxicity.					
30802890	3	77	theme	In	495:496	arg1	cytocompatibility					504:520	In vitro cytocompatibility	495:520	In vitro cytocompatibility	495:520	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	6	78	theme	MSC	1097:1099	arg1	differentiation					1101:1115	MSC differentiation	1097:1115	MSC differentiation	1097:1115	The scanning electron and atomic force microscopy revealed that BT NP/alginate 3D scaffold produced exhibited highly interconnected pores and surface nanotopography that were favorable for MSC differentiation.					
30802890	0	79	theme	3D	61:62	arg1	scaffold					64:71	nanoparticle/alginate 3D scaffold	39:71	nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation	39:118	In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.					
30802890	7	80	theme	3D	1334:1335	arg1	scaffold					1337:1344	the BT NP/alginate 3D scaffold	1315:1344	the BT NP/alginate 3D scaffold	1315:1344	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	2	81	theme	cell	473:476	arg1	differentiation					478:492	osteogenic stem cell differentiation	457:492	osteogenic stem cell differentiation	457:492	In the present study, we investigated whether barium titanate nanoparticles (BT NP) combined with alginate polymer would provide a new cytocompatible three-dimensional (3D) scaffold to induce osteogenic stem cell differentiation.					
30802890	3	82	theme	osteogenic	526:535	arg1	potential					553:561	osteogenic differentiation potential	526:561	osteogenic differentiation potential	526:561	In vitro cytocompatibility and osteogenic differentiation potential were investigated using human mesenchymal stem cells (MSC).					
30802890	0	83	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro evaluation of barium titanate nanoparticle/alginate 3D scaffold for osteogenic human stem cell differentiation.					
30802890	7	84	theme	BT	1319:1320	arg1	scaffold					1337:1344	the BT NP/alginate 3D scaffold	1315:1344	the BT NP/alginate 3D scaffold	1315:1344	Von Kossa staining showed mineralization nodules and MSCs morphology changed from spindle to cuboid shape after 21 d. Finally, BMP-2 and ALP mRNA were significantly upregulated on cells grown into the BT NP/alginate 3D scaffold.					
30802890	8	85	theme	supplements	1477:1487	arg1	addition					1454:1461	the addition	1450:1461	the addition of osteogenic supplements	1450:1487	Thus, the BT NP/alginate 3D scaffold showed an osteogenic differentiation induction potential, without the addition of osteogenic supplements.					
30784969	0	0	theme	efficient	79:87	arg1	agent					131:135	An efficient photocatalyst and effective antibacterial agent	76:135	ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent	33:135	Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.					
30784969	2	1	dep	spectroscopy	619:630	arg1	analyses					779:786	analyses	779:786	analyses	779:786	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	2	theme	X-ray	740:744	arg1	XPS					774:776	XPS	774:776	XPS	774:776	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	2	theme	X-ray	740:744	arg1	spectroscopy					760:771	X-ray photoelectron spectroscopy	740:771	X-ray photoelectron spectroscopy (XPS)	740:777	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	2	theme	X-ray	740:744	arg1	spectroscopy					619:630	13C nuclear magnetic resonance spectroscopy	588:630	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	1	3	theme	cl-Ch-pMAc	161:170	arg1	ZnO/CdSQDs					172:181	cl-Ch-pMAc@ZnO/CdSQDs	161:181	cl-Ch-pMAc@ZnO/CdSQDs	161:181	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	3	theme	cl-Ch-pMAc	161:170	arg1	nanocomposite					146:158	A novel nanocomposite	138:158	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs)	138:182	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	4	theme	quantum	258:264	arg1	dots					266:269	ZnO/CdS quantum dots	250:269	ZnO/CdS quantum dots	250:269	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	5	5	dep	B.	1227:1228	arg1	subtilis					1230:1237	B. subtilis	1227:1237	B. subtilis	1227:1237	Moreover, the composite exhibits excellent antibacterial activity towards E-coli and B. subtilis.					
30784969	2	6	theme	NMR	637:639	arg1	spectroscopy					641:652	13C NMR spectroscopy	633:652	13C NMR spectroscopy	633:652	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	6	theme	NMR	637:639	arg1	spectroscopy					619:630	13C nuclear magnetic resonance spectroscopy	588:630	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	1	7	theme	@	171:171	arg1	ZnO/CdSQDs					172:181	cl-Ch-pMAc@ZnO/CdSQDs	161:181	cl-Ch-pMAc@ZnO/CdSQDs	161:181	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	7	theme	@	171:171	arg1	nanocomposite					146:158	A novel nanocomposite	138:158	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs)	138:182	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	6	8	from	reduction	1383:1391	arg1	efficiency					1400:1409	its efficiency	1396:1409	its efficiency	1396:1409	Finally, the nanocomposite can be regenerated effectively with changing the solution pH and also shows 5 times reusability without significant reduction on its efficiency.					
30784969	1	9	theme	dots	266:269	arg1	fabrication					235:245	fabrication	235:245	fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	235:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	2	10	theme	13C	633:635	arg1	spectroscopy					641:652	13C NMR spectroscopy	633:652	13C NMR spectroscopy	633:652	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	10	theme	13C	633:635	arg1	spectroscopy					619:630	13C nuclear magnetic resonance spectroscopy	588:630	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	0	11	theme	photocatalyst	89:101	arg1	agent					131:135	An efficient photocatalyst and effective antibacterial agent	76:135	ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent	33:135	Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.					
30784969	4	12	theme	mass	1050:1053	arg1	LC-MS					1069:1073	LC-MS	1069:1073	LC-MS	1069:1073	Liquid chromatography mass spectroscopy (LC-MS) studies predict that small molecules are produced by degradation.					
30784969	4	12	theme	mass	1050:1053	arg1	spectroscopy					1055:1066	Liquid chromatography mass spectroscopy	1028:1066	Liquid chromatography mass spectroscopy (LC-MS) studies	1028:1082	Liquid chromatography mass spectroscopy (LC-MS) studies predict that small molecules are produced by degradation.					
30784969	0	13	theme	antibacterial	117:129	arg1	agent					131:135	An efficient photocatalyst and effective antibacterial agent	76:135	ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent	33:135	Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.					
30784969	2	14	theme	resolution-transmission	661:683	arg1	HR-TEM					706:711	HR-TEM	706:711	HR-TEM	706:711	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	14	theme	resolution-transmission	661:683	arg1	microscopy					694:703	high resolution-transmission electron microscopy	656:703	high resolution-transmission electron microscopy (HR-TEM)	656:712	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	14	theme	resolution-transmission	661:683	arg1	spectroscopy					619:630	13C nuclear magnetic resonance spectroscopy	588:630	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	15	theme	electron	685:692	arg1	HR-TEM					706:711	HR-TEM	706:711	HR-TEM	706:711	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	15	theme	electron	685:692	arg1	microscopy					694:703	high resolution-transmission electron microscopy	656:703	high resolution-transmission electron microscopy (HR-TEM)	656:712	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	15	theme	electron	685:692	arg1	spectroscopy					619:630	13C nuclear magnetic resonance spectroscopy	588:630	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	0	16	theme	effective	107:115	arg1	agent					131:135	An efficient photocatalyst and effective antibacterial agent	76:135	ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent	33:135	Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.					
30784969	2	17	theme	high	656:659	arg1	HR-TEM					706:711	HR-TEM	706:711	HR-TEM	706:711	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	17	theme	high	656:659	arg1	microscopy					694:703	high resolution-transmission electron microscopy	656:703	high resolution-transmission electron microscopy (HR-TEM)	656:712	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	17	theme	high	656:659	arg1	spectroscopy					619:630	13C nuclear magnetic resonance spectroscopy	588:630	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	0	18	link	crosslinked	54:64	arg1	chitosan					66:73	crosslinked chitosan	54:73	crosslinked chitosan	54:73	Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.					
30784969	4	19	theme	chromatography	1035:1048	arg1	LC-MS					1069:1073	LC-MS	1069:1073	LC-MS	1069:1073	Liquid chromatography mass spectroscopy (LC-MS) studies predict that small molecules are produced by degradation.					
30784969	4	19	theme	chromatography	1035:1048	arg1	spectroscopy					1055:1066	Liquid chromatography mass spectroscopy	1028:1066	Liquid chromatography mass spectroscopy (LC-MS) studies	1028:1082	Liquid chromatography mass spectroscopy (LC-MS) studies predict that small molecules are produced by degradation.					
30784969	3	20	theme	sunlight	1018:1025	arg1	exposure					1006:1013	the exposure	1002:1013	the exposure of sunlight	1002:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	2	21	theme	structural	453:462	arg1	properties					501:510	The structural, morphological and chemical/physical properties	449:510	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites	449:557	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	5	22	theme	excellent	1175:1183	arg1	activity					1199:1206	excellent antibacterial activity	1175:1206	excellent antibacterial activity towards E-coli and B. subtilis	1175:1237	Moreover, the composite exhibits excellent antibacterial activity towards E-coli and B. subtilis.					
30784969	2	23	theme	X-ray	715:719	arg1	spectroscopy					619:630	13C nuclear magnetic resonance spectroscopy	588:630	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	23	theme	X-ray	715:719	arg1	XRD					734:736	XRD	734:736	XRD	734:736	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	23	theme	X-ray	715:719	arg1	diffraction					721:731	X-ray diffraction	715:731	X-ray diffraction (XRD)	715:737	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	0	24	theme	Novel	0:4	arg1	nanocomposite					6:18	Novel nanocomposite	0:18	Novel nanocomposite	0:18	Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.					
30784969	1	25	theme	functionalized	286:299	arg1	poly					316:319	anionically functionalized chitosan [i.e. poly	274:319	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	274:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	25	theme	functionalized	286:299	arg1	acid					334:337	methacrylic acid	322:337	methacrylic acid	322:337	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	3	26	dep	dyes	892:895	arg1	[malachite					897:906	[malachite green	897:912	[malachite green (MG)	897:917	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	26	dep	dyes	892:895	arg1	MG					915:916	MG	915:916	MG	915:916	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	26	dep	dyes	892:895	arg1	SF					934:935	SF	934:935	SF	934:935	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	26	dep	dyes	892:895	arg1	phenol					979:984	toxic organic molecule 2,4-dichloro phenol	943:984	toxic organic molecule 2,4-dichloro phenol (2,4-DCP)	943:994	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	26	dep	dyes	892:895	arg1	dyes					892:895	cationic dyes	883:895	cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight	883:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	26	dep	dyes	892:895	arg1	2,4-DCP					987:993	2,4-DCP	987:993	2,4-DCP	987:993	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	26	dep	dyes	892:895	arg1	safranin					924:931	safranin	924:931	safranin (SF)]	924:937	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	4	27	theme	Liquid	1028:1033	arg1	LC-MS					1069:1073	LC-MS	1069:1073	LC-MS	1069:1073	Liquid chromatography mass spectroscopy (LC-MS) studies predict that small molecules are produced by degradation.					
30784969	4	27	theme	Liquid	1028:1033	arg1	spectroscopy					1055:1066	Liquid chromatography mass spectroscopy	1028:1066	Liquid chromatography mass spectroscopy (LC-MS) studies	1028:1082	Liquid chromatography mass spectroscopy (LC-MS) studies predict that small molecules are produced by degradation.					
30784969	3	28	theme	outstanding	820:830	arg1	efficacy					832:839	outstanding efficacy	820:839	outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight	820:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	1	29	theme	chitosan	301:308	arg1	poly					316:319	anionically functionalized chitosan [i.e. poly	274:319	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	274:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	29	theme	chitosan	301:308	arg1	acid					334:337	methacrylic acid	322:337	methacrylic acid	322:337	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	2	30	theme	13C	588:590	arg1	resonance					609:617	13C nuclear magnetic resonance	588:617	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	31	theme	nuclear	592:598	arg1	resonance					609:617	13C nuclear magnetic resonance	588:617	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	3	32	theme	molecule	957:964	arg1	2,4-DCP					987:993	2,4-DCP	987:993	2,4-DCP	987:993	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	32	theme	molecule	957:964	arg1	phenol					979:984	toxic organic molecule 2,4-dichloro phenol	943:984	toxic organic molecule 2,4-dichloro phenol (2,4-DCP)	943:994	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	32	theme	molecule	957:964	arg1	dyes					892:895	cationic dyes	883:895	cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight	883:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	4	33	theme	spectroscopy	1055:1066	arg1	studies					1076:1082	Liquid chromatography mass spectroscopy (LC-MS) studies	1028:1082	Liquid chromatography mass spectroscopy (LC-MS) studies	1028:1082	Liquid chromatography mass spectroscopy (LC-MS) studies predict that small molecules are produced by degradation.					
30784969	1	34	theme	[i.e.	310:314	arg1	poly					316:319	anionically functionalized chitosan [i.e. poly	274:319	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	274:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	34	theme	[i.e.	310:314	arg1	acid					334:337	methacrylic acid	322:337	methacrylic acid	322:337	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	6	35	theme	significant	1371:1381	arg1	reduction					1383:1391	significant reduction	1371:1391	significant reduction on its efficiency	1371:1409	Finally, the nanocomposite can be regenerated effectively with changing the solution pH and also shows 5 times reusability without significant reduction on its efficiency.					
30784969	1	36	link	crosslinked	340:350	arg1	cl-Ch-pMAc					362:371	cl-Ch-pMAc	362:371	cl-Ch-pMAc	362:371	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	36	link	crosslinked	340:350	arg1	chitosan					352:359	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan	274:359	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	274:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	3	37	theme	organic	949:955	arg1	2,4-DCP					987:993	2,4-DCP	987:993	2,4-DCP	987:993	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	37	theme	organic	949:955	arg1	phenol					979:984	toxic organic molecule 2,4-dichloro phenol	943:984	toxic organic molecule 2,4-dichloro phenol (2,4-DCP)	943:994	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	37	theme	organic	949:955	arg1	dyes					892:895	cationic dyes	883:895	cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight	883:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	2	38	theme	chemical/physical	483:499	arg1	properties					501:510	The structural, morphological and chemical/physical properties	449:510	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites	449:557	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	1	39	theme	poly	316:319	arg1	cl-Ch-pMAc					362:371	cl-Ch-pMAc	362:371	cl-Ch-pMAc	362:371	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	39	theme	poly	316:319	arg1	chitosan					352:359	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan	274:359	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	274:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	40	theme	diethylene	393:402	arg1	crosslinker					435:445	diethylene glycol dimethacrylate (DEGDMA) crosslinker]	393:446	diethylene glycol dimethacrylate (DEGDMA) crosslinker]	393:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	3	41	theme	toxic	943:947	arg1	2,4-DCP					987:993	2,4-DCP	987:993	2,4-DCP	987:993	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	41	theme	toxic	943:947	arg1	phenol					979:984	toxic organic molecule 2,4-dichloro phenol	943:984	toxic organic molecule 2,4-dichloro phenol (2,4-DCP)	943:994	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	41	theme	toxic	943:947	arg1	dyes					892:895	cationic dyes	883:895	cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight	883:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	1	42	theme	microwave	209:217	arg1	irradiation					219:229	microwave irradiation	209:229	microwave irradiation	209:229	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	3	43	theme	dyes	892:895	arg1	degradation					868:878	the photocatalytic degradation	849:878	the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight	849:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	1	44	theme	glycol	404:409	arg1	crosslinker					435:445	diethylene glycol dimethacrylate (DEGDMA) crosslinker]	393:446	diethylene glycol dimethacrylate (DEGDMA) crosslinker]	393:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	0	45	dep	embedded	45:52	arg1	chitosan					66:73	crosslinked chitosan	54:73	crosslinked chitosan	54:73	Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.					
30784969	0	46	theme	crosslinked	54:64	arg1	chitosan					66:73	crosslinked chitosan	54:73	crosslinked chitosan	54:73	Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.					
30784969	1	47	theme	methacrylic	322:332	arg1	poly					316:319	anionically functionalized chitosan [i.e. poly	274:319	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	274:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	47	theme	methacrylic	322:332	arg1	acid					334:337	methacrylic acid	322:337	methacrylic acid	322:337	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	48	theme	dimethacrylate	411:424	arg1	crosslinker					435:445	diethylene glycol dimethacrylate (DEGDMA) crosslinker]	393:446	diethylene glycol dimethacrylate (DEGDMA) crosslinker]	393:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	2	49	theme	nanocomposites	544:557	arg1	properties					501:510	The structural, morphological and chemical/physical properties	449:510	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites	449:557	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	1	50	from	chitosan	352:359	arg1	presence					381:388	the presence	377:388	the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	377:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	5	51	theme	antibacterial	1185:1197	arg1	activity					1199:1206	excellent antibacterial activity	1175:1206	excellent antibacterial activity towards E-coli and B. subtilis	1175:1237	Moreover, the composite exhibits excellent antibacterial activity towards E-coli and B. subtilis.					
30784969	2	52	theme	resonance	609:617	arg1	spectroscopy					641:652	13C NMR spectroscopy	633:652	13C NMR spectroscopy	633:652	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	52	theme	resonance	609:617	arg1	diffraction					721:731	X-ray diffraction	715:731	X-ray diffraction (XRD)	715:737	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	52	theme	resonance	609:617	arg1	spectroscopy					760:771	X-ray photoelectron spectroscopy	740:771	X-ray photoelectron spectroscopy (XPS)	740:777	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	52	theme	resonance	609:617	arg1	microscopy					694:703	high resolution-transmission electron microscopy	656:703	high resolution-transmission electron microscopy (HR-TEM)	656:712	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	52	theme	resonance	609:617	arg1	spectroscopy					619:630	13C nuclear magnetic resonance spectroscopy	588:630	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	0	53	dep	ZnO/CdS	33:39	arg1	agent					131:135	An efficient photocatalyst and effective antibacterial agent	76:135	ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent	33:135	Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.					
30784969	3	54	theme	2,4-dichloro	966:977	arg1	2,4-DCP					987:993	2,4-DCP	987:993	2,4-DCP	987:993	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	54	theme	2,4-dichloro	966:977	arg1	phenol					979:984	toxic organic molecule 2,4-dichloro phenol	943:984	toxic organic molecule 2,4-dichloro phenol (2,4-DCP)	943:994	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	54	theme	2,4-dichloro	966:977	arg1	dyes					892:895	cationic dyes	883:895	cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight	883:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	6	55	theme	solution	1316:1323	arg1	pH					1325:1326	the solution pH	1312:1326	the solution pH	1312:1326	Finally, the nanocomposite can be regenerated effectively with changing the solution pH and also shows 5 times reusability without significant reduction on its efficiency.					
30784969	4	56	theme	small	1097:1101	arg1	molecules					1103:1111	small molecules	1097:1111	small molecules	1097:1111	Liquid chromatography mass spectroscopy (LC-MS) studies predict that small molecules are produced by degradation.					
30784969	2	57	theme	magnetic	600:607	arg1	resonance					609:617	13C nuclear magnetic resonance	588:617	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	1	58	theme	DEGDMA	427:432	arg1	crosslinker					435:445	diethylene glycol dimethacrylate (DEGDMA) crosslinker]	393:446	diethylene glycol dimethacrylate (DEGDMA) crosslinker]	393:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	2	59	link	crosslinked	515:525	arg1	chitosan					527:534	crosslinked chitosan	515:534	crosslinked chitosan	515:534	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	6	60	theme	reusability	1351:1361	arg1	times					1345:1349	5 times	1343:1349	5 times reusability without significant reduction on its efficiency	1343:1409	Finally, the nanocomposite can be regenerated effectively with changing the solution pH and also shows 5 times reusability without significant reduction on its efficiency.					
30784969	1	61	from	fabrication	235:245	arg1	cl-Ch-pMAc					362:371	cl-Ch-pMAc	362:371	cl-Ch-pMAc	362:371	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	61	from	fabrication	235:245	arg1	chitosan					352:359	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan	274:359	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	274:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	3	62	theme	cationic	883:890	arg1	[malachite					897:906	[malachite green	897:912	[malachite green (MG)	897:917	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	62	theme	cationic	883:890	arg1	safranin					924:931	safranin	924:931	safranin (SF)]	924:937	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	62	theme	cationic	883:890	arg1	phenol					979:984	toxic organic molecule 2,4-dichloro phenol	943:984	toxic organic molecule 2,4-dichloro phenol (2,4-DCP)	943:994	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	3	62	theme	cationic	883:890	arg1	dyes					892:895	cationic dyes	883:895	cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight	883:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	1	63	theme	novel	140:144	arg1	ZnO/CdSQDs					172:181	cl-Ch-pMAc@ZnO/CdSQDs	161:181	cl-Ch-pMAc@ZnO/CdSQDs	161:181	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	63	theme	novel	140:144	arg1	nanocomposite					146:158	A novel nanocomposite	138:158	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs)	138:182	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	3	64	theme	photocatalytic	853:866	arg1	degradation					868:878	the photocatalytic degradation	849:878	the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight	849:1025	The nanocomposite demonstrates outstanding efficacy towards the photocatalytic degradation of cationic dyes [malachite green (MG), and safranin (SF)] and toxic organic molecule 2,4-dichloro phenol (2,4-DCP) under the exposure of sunlight.					
30784969	2	65	theme	chitosan	527:534	arg1	properties					501:510	The structural, morphological and chemical/physical properties	449:510	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites	449:557	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	1	66	theme	crosslinker	435:445	arg1	presence					381:388	the presence	377:388	the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	377:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	2	67	theme	morphological	465:477	arg1	properties					501:510	The structural, morphological and chemical/physical properties	449:510	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites	449:557	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	1	68	theme	crosslinked	340:350	arg1	cl-Ch-pMAc					362:371	cl-Ch-pMAc	362:371	cl-Ch-pMAc	362:371	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	1	68	theme	crosslinked	340:350	arg1	chitosan					352:359	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan	274:359	anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]	274:446	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
30784969	2	69	theme	photoelectron	746:758	arg1	XPS					774:776	XPS	774:776	XPS	774:776	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	69	theme	photoelectron	746:758	arg1	spectroscopy					760:771	X-ray photoelectron spectroscopy	740:771	X-ray photoelectron spectroscopy (XPS)	740:777	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	69	theme	photoelectron	746:758	arg1	spectroscopy					619:630	13C nuclear magnetic resonance spectroscopy	588:630	13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy)	588:653	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	2	70	theme	crosslinked	515:525	arg1	chitosan					527:534	crosslinked chitosan	515:534	crosslinked chitosan	515:534	The structural, morphological and chemical/physical properties of crosslinked chitosan and the nanocomposites have been investigated using 13C nuclear magnetic resonance spectroscopy (13C NMR spectroscopy), high resolution-transmission electron microscopy (HR-TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) analyses.					
30784969	1	71	theme	ZnO/CdS	250:256	arg1	dots					266:269	ZnO/CdS quantum dots	250:269	ZnO/CdS quantum dots	250:269	A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker].					
31461665	0	0	theme	drug	94:97	arg1	delivery					99:106	ultrasound-triggered drug delivery	73:106	ultrasound-triggered drug delivery	73:106	Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery.					
31461665	1	1	theme	innovative	283:292	arg1	tool					321:324	a useful innovative diagnostic and therapeutic tool	274:324	a useful innovative diagnostic and therapeutic tool	274:324	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	1	theme	innovative	283:292	arg1	potential					261:269	potential	261:269	potential	261:269	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	9	2	theme	HE	1530:1531	arg1	staining					1533:1540	HE staining	1530:1540	HE staining	1530:1540	In these treatments, notable damage to brain was not observed, which was assessed by HE staining and denatured neuron staining.					
31461665	0	3	theme	ultrasound-triggered	73:92	arg1	delivery					99:106	ultrasound-triggered drug delivery	73:106	ultrasound-triggered drug delivery	73:106	Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery.					
31461665	1	4	theme	diagnostic	294:303	arg1	tool					321:324	a useful innovative diagnostic and therapeutic tool	274:324	a useful innovative diagnostic and therapeutic tool	274:324	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	4	theme	diagnostic	294:303	arg1	potential					261:269	potential	261:269	potential	261:269	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	5	theme	contrast	151:158	arg1	microbubbles					167:178	microbubbles	167:178	microbubbles	167:178	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	5	theme	contrast	151:158	arg1	agent					160:164	US contrast agent	148:164	US contrast agent (microbubbles, MBs)	148:184	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	0	6	from	Effects	0:6	arg1	stability					31:39	stability	31:39	stability of lipid-based microbubbles and ultrasound-triggered drug delivery	31:106	Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery.					
31461665	7	7	dep	MBs	1135:1137	arg1	MB-C4F10					1152:1159	MB-C4F10	1152:1159	MB-C4F10	1152:1159	Perfluoropropane and perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10) showed sustained US imaging in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF6).					
31461665	7	7	dep	MBs	1135:1137	arg1	MB-C3F8					1140:1146	MB-C3F8	1140:1146	MB-C3F8	1140:1146	Perfluoropropane and perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10) showed sustained US imaging in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF6).					
31461665	3	8	theme	anionic	530:536	arg1	phospholipids					538:550	anionic phospholipids	530:550	anionic phospholipids	530:550	We reported that MBs composed of neutral, anionic phospholipids, and polyethylene glycol-conjugated phospholipids at appropriate ratios were highly stable for US imaging.					
31461665	6	9	theme	delivery	1074:1081	arg1	system					1083:1088	an efficient drug delivery system	1056:1088	an efficient drug delivery system	1056:1088	In addition, we assessed the effects of gas encapsulated in MBs on brain-targeted drug delivery, because the brain requires an efficient drug delivery system.					
31461665	0	10	theme	delivery	99:106	arg1	stability					31:39	stability	31:39	stability of lipid-based microbubbles and ultrasound-triggered drug delivery	31:106	Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery.					
31461665	1	11	theme	therapeutic	309:319	arg1	tool					321:324	a useful innovative diagnostic and therapeutic tool	274:324	a useful innovative diagnostic and therapeutic tool	274:324	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	11	theme	therapeutic	309:319	arg1	potential					261:269	potential	261:269	potential	261:269	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	6	12	from	effects	961:967	arg1	delivery					1019:1026	brain-targeted drug delivery	999:1026	brain-targeted drug delivery	999:1026	In addition, we assessed the effects of gas encapsulated in MBs on brain-targeted drug delivery, because the brain requires an efficient drug delivery system.					
31461665	4	13	theme	delivery	722:729	arg1	efficacy					731:738	drug delivery efficacy	717:738	drug delivery efficacy	717:738	However, the effects of encapsulated gas on stability and drug delivery efficacy have not been characterized.					
31461665	2	14	theme	delivery	469:476	arg1	efficacy					478:485	drug delivery efficacy	464:485	drug delivery efficacy	464:485	Previous studies have evaluated how particle size or shell components of MBs affect their physical characteristics, imaging ability, and drug delivery efficacy.					
31461665	9	15	theme	neuron	1556:1561	arg1	staining					1563:1570	denatured neuron staining	1546:1570	denatured neuron staining	1546:1570	In these treatments, notable damage to brain was not observed, which was assessed by HE staining and denatured neuron staining.					
31461665	5	16	dep	imaging	885:891	arg1	E9					900:901	LOGIQ E9	894:901	LOGIQ E9 with ML6-15 probe, MI 0.20	894:928	Therefore, we developed several gas-loaded MBs with identical shell compositions and assessed their stability by US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20).					
31461665	1	17	theme	agent	160:164	arg1	gas					196:198	gas	196:198	gas stabilized by a shell such as phospholipids or proteins	196:254	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	17	theme	agent	160:164	arg1	combination					113:123	The combination	109:123	The combination	109:123	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	2	18	theme	drug	464:467	arg1	efficacy					478:485	drug delivery efficacy	464:485	drug delivery efficacy	464:485	Previous studies have evaluated how particle size or shell components of MBs affect their physical characteristics, imaging ability, and drug delivery efficacy.					
31461665	5	19	theme	US	882:883	arg1	imaging					885:891	US imaging	882:891	US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20)	882:929	Therefore, we developed several gas-loaded MBs with identical shell compositions and assessed their stability by US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20).					
31461665	5	20	with	E9	900:901	arg1	probe					915:919	ML6-15 probe	908:919	ML6-15 probe	908:919	Therefore, we developed several gas-loaded MBs with identical shell compositions and assessed their stability by US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20).					
31461665	5	20	with	E9	900:901	arg1	MI					922:923	MI 0.20	922:928	MI 0.20	922:928	Therefore, we developed several gas-loaded MBs with identical shell compositions and assessed their stability by US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20).					
31461665	1	21	dep	Ultrasound	128:137	arg1	US					140:141	US	140:141	US	140:141	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	6	22	theme	drug	1014:1017	arg1	delivery					1019:1026	brain-targeted drug delivery	999:1026	brain-targeted drug delivery	999:1026	In addition, we assessed the effects of gas encapsulated in MBs on brain-targeted drug delivery, because the brain requires an efficient drug delivery system.					
31461665	2	23	theme	imaging	443:449	arg1	ability					451:457	imaging ability	443:457	imaging ability	443:457	Previous studies have evaluated how particle size or shell components of MBs affect their physical characteristics, imaging ability, and drug delivery efficacy.					
31461665	7	24	theme	sustained	1169:1177	arg1	imaging					1182:1188	sustained US imaging	1169:1188	sustained US imaging	1169:1188	Perfluoropropane and perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10) showed sustained US imaging in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF6).					
31461665	8	25	theme	MB-C4F10	1304:1311	arg1	treatment					1279:1287	treatment	1279:1287	treatment of MB-C3F8 and MB-C4F10 with non-focused US	1279:1331	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	6	26	theme	brain-targeted	999:1012	arg1	delivery					1019:1026	brain-targeted drug delivery	999:1026	brain-targeted drug delivery	999:1026	In addition, we assessed the effects of gas encapsulated in MBs on brain-targeted drug delivery, because the brain requires an efficient drug delivery system.					
31461665	2	27	theme	MBs	400:402	arg1	size					372:375	particle size	363:375	particle size	363:375	Previous studies have evaluated how particle size or shell components of MBs affect their physical characteristics, imaging ability, and drug delivery efficacy.					
31461665	2	27	theme	MBs	400:402	arg1	components					386:395	shell components	380:395	shell components of MBs	380:402	Previous studies have evaluated how particle size or shell components of MBs affect their physical characteristics, imaging ability, and drug delivery efficacy.					
31461665	9	28	theme	notable	1466:1472	arg1	damage					1474:1479	notable damage	1466:1479	notable damage to brain	1466:1488	In these treatments, notable damage to brain was not observed, which was assessed by HE staining and denatured neuron staining.					
31461665	8	29	theme	blue	1361:1364	arg1	Evans					1355:1359	Evans blue	1355:1364	Evans blue	1355:1364	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	8	29	theme	blue	1361:1364	arg1	drug					1393:1396	a model drug	1385:1396	a model drug	1385:1396	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	10	30	with	production	1661:1670	arg1	stability					1689:1697	high stability	1684:1697	high stability	1684:1697	Our results suggested that perfluoropropane and perfluorobutane could be useful for the production of MBs with high stability to allow for US imaging and drug delivery.					
31461665	0	31	theme	gas	24:26	arg1	Effects					0:6	Effects	0:6	Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery	0:106	Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery.					
31461665	4	32	theme	drug	717:720	arg1	efficacy					731:738	drug delivery efficacy	717:738	drug delivery efficacy	717:738	However, the effects of encapsulated gas on stability and drug delivery efficacy have not been characterized.					
31461665	2	33	theme	shell	380:384	arg1	components					386:395	shell components	380:395	shell components of MBs	380:402	Previous studies have evaluated how particle size or shell components of MBs affect their physical characteristics, imaging ability, and drug delivery efficacy.					
31461665	0	34	theme	encapsulated	11:22	arg1	gas					24:26	encapsulated gas	11:26	encapsulated gas	11:26	Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery.					
31461665	8	35	used	used	1377:1380	arg2	Evans					1355:1359	Evans blue	1355:1364	Evans blue	1355:1364	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	8	35	used	used	1377:1380	arg2	drug					1393:1396	a model drug	1385:1396	a model drug	1385:1396	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	8	36	theme	MB-C3F8	1292:1298	arg1	treatment					1279:1287	treatment	1279:1287	treatment of MB-C3F8 and MB-C4F10 with non-focused US	1279:1331	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	1	37	contain	has	257:259	arg1	gas					196:198	gas	196:198	gas stabilized by a shell such as phospholipids or proteins	196:254	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	37	contain	has	257:259	arg2	potential					261:269	potential	261:269	potential	261:269	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	37	contain	has	257:259	arg2	tool					321:324	a useful innovative diagnostic and therapeutic tool	274:324	a useful innovative diagnostic and therapeutic tool	274:324	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	37	contain	has	257:259	arg1	combination					113:123	The combination	109:123	The combination	109:123	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	2	38	theme	physical	417:424	arg1	characteristics					426:440	their physical characteristics	411:440	their physical characteristics	411:440	Previous studies have evaluated how particle size or shell components of MBs affect their physical characteristics, imaging ability, and drug delivery efficacy.					
31461665	6	39	theme	efficient	1059:1067	arg1	system					1083:1088	an efficient drug delivery system	1056:1088	an efficient drug delivery system	1056:1088	In addition, we assessed the effects of gas encapsulated in MBs on brain-targeted drug delivery, because the brain requires an efficient drug delivery system.					
31461665	1	40	dep	microbubbles	167:178	arg1	MBs					181:183	MBs	181:183	MBs	181:183	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	7	41	theme	hexafluoride-loaded	1232:1250	arg1	MB-SF6					1257:1262	MB-SF6	1257:1262	MB-SF6	1257:1262	Perfluoropropane and perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10) showed sustained US imaging in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF6).					
31461665	7	41	theme	hexafluoride-loaded	1232:1250	arg1	MBs					1252:1254	sulfur hexafluoride-loaded MBs	1225:1254	sulfur hexafluoride-loaded MBs (MB-SF6)	1225:1263	Perfluoropropane and perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10) showed sustained US imaging in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF6).					
31461665	5	42	theme	shell	831:835	arg1	compositions					837:848	identical shell compositions	821:848	identical shell compositions	821:848	Therefore, we developed several gas-loaded MBs with identical shell compositions and assessed their stability by US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20).					
31461665	3	43	theme	appropriate	605:615	arg1	ratios					617:622	appropriate ratios	605:622	appropriate ratios	605:622	We reported that MBs composed of neutral, anionic phospholipids, and polyethylene glycol-conjugated phospholipids at appropriate ratios were highly stable for US imaging.					
31461665	10	44	theme	high	1684:1687	arg1	stability					1689:1697	high stability	1684:1697	high stability	1684:1697	Our results suggested that perfluoropropane and perfluorobutane could be useful for the production of MBs with high stability to allow for US imaging and drug delivery.					
31461665	2	45	theme	particle	363:370	arg1	size					372:375	particle size	363:375	particle size	363:375	Previous studies have evaluated how particle size or shell components of MBs affect their physical characteristics, imaging ability, and drug delivery efficacy.					
31461665	0	46	theme	lipid-based	44:54	arg1	microbubbles					56:67	lipid-based microbubbles	44:67	lipid-based microbubbles	44:67	Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery.					
31461665	8	47	theme	model	1387:1391	arg1	Evans					1355:1359	Evans blue	1355:1364	Evans blue	1355:1364	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	8	47	theme	model	1387:1391	arg1	drug					1393:1396	a model drug	1385:1396	a model drug	1385:1396	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	6	48	theme	gas	972:974	arg1	effects					961:967	the effects	957:967	the effects of gas encapsulated in MBs on brain-targeted drug delivery	957:1026	In addition, we assessed the effects of gas encapsulated in MBs on brain-targeted drug delivery, because the brain requires an efficient drug delivery system.					
31461665	1	49	dep	agent	160:164	arg1	US					148:149	US	148:149	US	148:149	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	6	50	theme	drug	1069:1072	arg1	system					1083:1088	an efficient drug delivery system	1056:1088	an efficient drug delivery system	1056:1088	In addition, we assessed the effects of gas encapsulated in MBs on brain-targeted drug delivery, because the brain requires an efficient drug delivery system.					
31461665	3	51	theme	US	647:648	arg1	imaging					650:656	US imaging	647:656	US imaging	647:656	We reported that MBs composed of neutral, anionic phospholipids, and polyethylene glycol-conjugated phospholipids at appropriate ratios were highly stable for US imaging.					
31461665	9	52	theme	denatured	1546:1554	arg1	staining					1563:1570	denatured neuron staining	1546:1570	denatured neuron staining	1546:1570	In these treatments, notable damage to brain was not observed, which was assessed by HE staining and denatured neuron staining.					
31461665	1	53	theme	Ultrasound	128:137	arg1	gas					196:198	gas	196:198	gas stabilized by a shell such as phospholipids or proteins	196:254	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	53	theme	Ultrasound	128:137	arg1	combination					113:123	The combination	109:123	The combination	109:123	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	10	54	theme	US	1712:1713	arg1	imaging					1715:1721	US imaging	1712:1721	US imaging	1712:1721	Our results suggested that perfluoropropane and perfluorobutane could be useful for the production of MBs with high stability to allow for US imaging and drug delivery.					
31461665	9	55	located	observed	1498:1505	arg1	treatments					1454:1463	these treatments	1448:1463	these treatments	1448:1463	In these treatments, notable damage to brain was not observed, which was assessed by HE staining and denatured neuron staining.					
31461665	9	55	located	observed	1498:1505	arg2	damage					1474:1479	notable damage	1466:1479	notable damage to brain	1466:1488	In these treatments, notable damage to brain was not observed, which was assessed by HE staining and denatured neuron staining.					
31461665	10	56	theme	drug	1727:1730	arg1	delivery					1732:1739	drug delivery	1727:1739	drug delivery	1727:1739	Our results suggested that perfluoropropane and perfluorobutane could be useful for the production of MBs with high stability to allow for US imaging and drug delivery.					
31461665	4	57	theme	gas	696:698	arg1	effects					672:678	the effects	668:678	the effects of encapsulated gas on stability and drug delivery efficacy	668:738	However, the effects of encapsulated gas on stability and drug delivery efficacy have not been characterized.					
31461665	10	58	theme	MBs	1675:1677	arg1	production					1661:1670	the production	1657:1670	the production of MBs with high stability	1657:1697	Our results suggested that perfluoropropane and perfluorobutane could be useful for the production of MBs with high stability to allow for US imaging and drug delivery.					
31461665	7	59	theme	sulfur	1225:1230	arg1	MB-SF6					1257:1262	MB-SF6	1257:1262	MB-SF6	1257:1262	Perfluoropropane and perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10) showed sustained US imaging in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF6).					
31461665	7	59	theme	sulfur	1225:1230	arg1	MBs					1252:1254	sulfur hexafluoride-loaded MBs	1225:1254	sulfur hexafluoride-loaded MBs (MB-SF6)	1225:1263	Perfluoropropane and perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10) showed sustained US imaging in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF6).					
31461665	5	60	theme	several	793:799	arg1	MBs					812:814	several gas-loaded MBs	793:814	several gas-loaded MBs	793:814	Therefore, we developed several gas-loaded MBs with identical shell compositions and assessed their stability by US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20).					
31461665	4	61	theme	encapsulated	683:694	arg1	gas					696:698	encapsulated gas	683:698	encapsulated gas	683:698	However, the effects of encapsulated gas on stability and drug delivery efficacy have not been characterized.					
31461665	5	62	theme	LOGIQ	894:898	arg1	E9					900:901	LOGIQ E9	894:901	LOGIQ E9 with ML6-15 probe, MI 0.20	894:928	Therefore, we developed several gas-loaded MBs with identical shell compositions and assessed their stability by US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20).					
31461665	7	63	theme	US	1179:1180	arg1	imaging					1182:1188	sustained US imaging	1169:1188	sustained US imaging	1169:1188	Perfluoropropane and perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10) showed sustained US imaging in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF6).					
31461665	5	64	theme	identical	821:829	arg1	compositions					837:848	identical shell compositions	821:848	identical shell compositions	821:848	Therefore, we developed several gas-loaded MBs with identical shell compositions and assessed their stability by US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20).					
31461665	3	65	theme	polyethylene	557:568	arg1	phospholipids					588:600	polyethylene glycol-conjugated phospholipids	557:600	polyethylene glycol-conjugated phospholipids	557:600	We reported that MBs composed of neutral, anionic phospholipids, and polyethylene glycol-conjugated phospholipids at appropriate ratios were highly stable for US imaging.					
31461665	5	66	theme	gas-loaded	801:810	arg1	MBs					812:814	several gas-loaded MBs	793:814	several gas-loaded MBs	793:814	Therefore, we developed several gas-loaded MBs with identical shell compositions and assessed their stability by US imaging (LOGIQ E9 with ML6-15 probe, MI 0.20).					
31461665	2	67	theme	Previous	327:334	arg1	studies					336:342	Previous studies	327:342	Previous studies	327:342	Previous studies have evaluated how particle size or shell components of MBs affect their physical characteristics, imaging ability, and drug delivery efficacy.					
31461665	4	68	from	effects	672:678	arg1	efficacy					731:738	drug delivery efficacy	717:738	drug delivery efficacy	717:738	However, the effects of encapsulated gas on stability and drug delivery efficacy have not been characterized.					
31461665	4	68	from	effects	672:678	arg1	stability					703:711	stability	703:711	stability	703:711	However, the effects of encapsulated gas on stability and drug delivery efficacy have not been characterized.					
31461665	8	69	with	treatment	1279:1287	arg1	US					1330:1331	US	1330:1331	US	1330:1331	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	0	70	theme	microbubbles	56:67	arg1	stability					31:39	stability	31:39	stability of lipid-based microbubbles and ultrasound-triggered drug delivery	31:106	Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery.					
31461665	1	71	theme	useful	276:281	arg1	tool					321:324	a useful innovative diagnostic and therapeutic tool	274:324	a useful innovative diagnostic and therapeutic tool	274:324	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	1	71	theme	useful	276:281	arg1	potential					261:269	potential	261:269	potential	261:269	The combination of Ultrasound (US) and US contrast agent (microbubbles, MBs), which is gas stabilized by a shell such as phospholipids or proteins, has potential as a useful innovative diagnostic and therapeutic tool.					
31461665	3	72	theme	glycol-conjugated	570:586	arg1	phospholipids					588:600	polyethylene glycol-conjugated phospholipids	557:600	polyethylene glycol-conjugated phospholipids	557:600	We reported that MBs composed of neutral, anionic phospholipids, and polyethylene glycol-conjugated phospholipids at appropriate ratios were highly stable for US imaging.					
31461665	7	73	theme	perfluorobutane-loaded	1112:1133	arg1	MBs					1135:1137	perfluorobutane-loaded MBs	1112:1137	perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10)	1112:1160	Perfluoropropane and perfluorobutane-loaded MBs (MB-C3F8 and MB-C4F10) showed sustained US imaging in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF6).					
31461665	8	74	theme	greater	1417:1423	arg1	extent					1425:1430	a greater extent	1415:1430	a greater extent than MB-SF6	1415:1442	In addition, treatment of MB-C3F8 and MB-C4F10 with non-focused US efficiently delivered Evans blue, which was used as a model drug, to the brain to a greater extent than MB-SF6.					
31461665	3	75	from	ratios	617:622	arg1	MBs					505:507	MBs	505:507	MBs composed of neutral	505:527	We reported that MBs composed of neutral, anionic phospholipids, and polyethylene glycol-conjugated phospholipids at appropriate ratios were highly stable for US imaging.					
31461665	3	75	from	ratios	617:622	arg1	phospholipids					588:600	polyethylene glycol-conjugated phospholipids	557:600	polyethylene glycol-conjugated phospholipids	557:600	We reported that MBs composed of neutral, anionic phospholipids, and polyethylene glycol-conjugated phospholipids at appropriate ratios were highly stable for US imaging.					
31461665	3	75	from	ratios	617:622	arg1	phospholipids					538:550	anionic phospholipids	530:550	anionic phospholipids	530:550	We reported that MBs composed of neutral, anionic phospholipids, and polyethylene glycol-conjugated phospholipids at appropriate ratios were highly stable for US imaging.					
29589997	0	0	theme	Biometric	60:68	arg1	Implications					127:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	2	1	theme	growing	456:462	arg1	attention					464:472	a growing attention	454:472	a growing attention as candidate ingredients for aquafeeds	454:511	Due to minimal environmental impact, compared with most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds.					
29589997	4	2	theme	multidisciplinary	652:668	arg1	approach					670:677	a multidisciplinary approach	650:677	a multidisciplinary approach	650:677	Through a multidisciplinary approach, the major biological responses of fish to the new diets were assessed.					
29589997	1	3	theme	meal	211:214	arg1	fish					206:209	fish meal	206:214	fish meal	206:214	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	2	4	theme	minimal	358:364	arg1	impact					380:385	minimal environmental impact	358:385	minimal environmental impact	358:385	Due to minimal environmental impact, compared with most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds.					
29589997	5	5	theme	stress	841:846	arg1	response					848:855	stress response	841:855	stress response	841:855	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	5	6	theme	larval	1018:1023	arg1	rearing					1025:1031	zebrafish larval rearing	1008:1031	zebrafish larval rearing	1008:1031	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	0	7	theme	Histological	71:82	arg1	Implications					127:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	1	8	theme	fish	316:319	arg1	health					321:326	fish health and welfare standards	316:348	health	321:326	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	3	9	theme	present	518:524	arg1	study					526:530	The present study	514:530	The present study	514:530	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	5	10	theme	macromolecular	927:940	arg1	composition					942:952	liver macromolecular composition	921:952	liver macromolecular composition	921:952	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	1	11	theme	fish	220:223	arg1	oil					225:227	fish oil	220:227	fish oil	220:227	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	6	12	theme	fish	1143:1146	arg1	rearing					1132:1138	the rearing	1128:1138	the rearing of fish	1128:1146	However, further studies are necessary to better understand the use of this insect species in the rearing of fish.					
29589997	6	13	theme	further	1043:1049	arg1	studies					1051:1057	further studies	1043:1057	further studies	1043:1057	However, further studies are necessary to better understand the use of this insect species in the rearing of fish.					
29589997	0	14	theme	Spectroscopic	85:97	arg1	Implications					127:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	6	15	theme	species	1117:1123	arg1	use					1098:1100	the use	1094:1100	the use of this insect species in the rearing of fish	1094:1146	However, further studies are necessary to better understand the use of this insect species in the rearing of fish.					
29589997	3	16	from	application	579:589	arg1	rearing					633:639	zebrafish larval rearing	616:639	zebrafish larval rearing	616:639	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	1	17	theme	desirable	143:151	arg1	goal					153:156	A desirable goal	141:156	A desirable goal of the aquaculture sector	141:182	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	2	18	theme	candidate	477:485	arg1	ingredients					487:497	candidate ingredients	477:497	candidate ingredients for aquafeeds	477:511	Due to minimal environmental impact, compared with most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds.					
29589997	1	19	theme	welfare	332:338	arg1	standards					340:348	fish health and welfare standards	316:348	standards	340:348	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	4	20	theme	new	726:728	arg1	diets					730:734	the new diets	722:734	the new diets	722:734	Through a multidisciplinary approach, the major biological responses of fish to the new diets were assessed.					
29589997	5	21	theme	genes	810:814	arg1	biometry					762:769	biometry	762:769	biometry	762:769	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	5	21	theme	genes	810:814	arg1	expression					796:805	expression	796:805	expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition	796:952	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	2	22	theme	feed	420:423	arg1	commodities					425:435	most conventional feed commodities	402:435	most conventional feed commodities	402:435	Due to minimal environmental impact, compared with most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds.					
29589997	2	23	theme	conventional	407:418	arg1	commodities					425:435	most conventional feed commodities	402:435	most conventional feed commodities	402:435	Due to minimal environmental impact, compared with most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds.					
29589997	5	24	theme	zebrafish	1008:1016	arg1	rearing					1025:1031	zebrafish larval rearing	1008:1031	zebrafish larval rearing	1008:1031	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	4	25	theme	biological	690:699	arg1	responses					701:709	the major biological responses	680:709	the major biological responses of fish to the new diets	680:734	Through a multidisciplinary approach, the major biological responses of fish to the new diets were assessed.					
29589997	6	26	from	use	1098:1100	arg1	rearing					1132:1138	the rearing	1128:1138	the rearing of fish	1128:1146	However, further studies are necessary to better understand the use of this insect species in the rearing of fish.					
29589997	0	27	theme	Black	21:25	arg1	Fly					35:37	Black Soldier Fly	21:37	Black Soldier Fly (Hermetia illucens)	21:57	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	0	27	theme	Black	21:25	arg1	illucens					49:56	Hermetia illucens	40:56	Hermetia illucens	40:56	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	5	28	theme	biometry	762:769	arg1	Results					751:757	Results	751:757	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition	751:952	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	0	29	theme	Biochemical	100:110	arg1	Implications					127:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	4	30	theme	fish	714:717	arg1	responses					701:709	the major biological responses	680:709	the major biological responses of fish to the new diets	680:734	Through a multidisciplinary approach, the major biological responses of fish to the new diets were assessed.					
29589997	1	31	theme	aquaculture	165:175	arg1	sector					177:182	the aquaculture sector	161:182	the aquaculture sector	161:182	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	5	32	theme	fatty	772:776	arg1	biometry					762:769	biometry	762:769	biometry	762:769	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	5	32	theme	fatty	772:776	arg1	composition					783:793	fatty acid composition	772:793	fatty acid composition	772:793	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	1	33	theme	sustainable	239:249	arg1	ingredients					295:305	more sustainable, cost-effective, and environmental friendly ingredients	234:305	more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards	234:348	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	5	34	theme	chitinolytic	876:887	arg1	activity					889:896	chitinolytic activity	876:896	chitinolytic activity	876:896	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	3	35	theme	insect	601:606	arg1	diet					608:611	a 100% insect diet	594:611	a 100% insect diet	594:611	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	1	36	theme	sector	177:182	arg1	goal					153:156	A desirable goal	141:156	A desirable goal of the aquaculture sector	141:182	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	3	37	theme	diet	608:611	arg1	application					579:589	the possible application	566:589	the possible application of a 100% insect diet in zebrafish larval rearing	566:639	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	5	38	theme	insect	990:995	arg1	larvae					997:1002	insect larvae	990:1002	insect larvae	990:1002	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	0	39	theme	Soldier	27:33	arg1	Fly					35:37	Black Soldier Fly	21:37	Black Soldier Fly (Hermetia illucens)	21:57	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	0	39	theme	Soldier	27:33	arg1	illucens					49:56	Hermetia illucens	40:56	Hermetia illucens	40:56	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	1	40	theme	cost-effective	252:265	arg1	ingredients					295:305	more sustainable, cost-effective, and environmental friendly ingredients	234:305	more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards	234:348	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	3	41	theme	100	596:598	arg1	%					599:599	%	599:599	%	599:599	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	2	42	theme	most	402:405	arg1	commodities					425:435	most conventional feed commodities	402:435	most conventional feed commodities	402:435	Due to minimal environmental impact, compared with most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds.					
29589997	2	43	theme	environmental	366:378	arg1	impact					380:385	minimal environmental impact	358:385	minimal environmental impact	358:385	Due to minimal environmental impact, compared with most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds.					
29589997	0	44	theme	Hermetia	40:47	arg1	Fly					35:37	Black Soldier Fly	21:37	Black Soldier Fly (Hermetia illucens)	21:57	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	0	44	theme	Hermetia	40:47	arg1	illucens					49:56	Hermetia illucens	40:56	Hermetia illucens	40:56	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	3	45	theme	%	599:599	arg1	diet					608:611	a 100% insect diet	594:611	a 100% insect diet	594:611	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	3	46	theme	first	554:558	arg1	time					560:563	the first time	550:563	the first time	550:563	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	0	47	theme	Molecular	117:125	arg1	Implications					127:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
29589997	3	48	theme	larval	626:631	arg1	rearing					633:639	zebrafish larval rearing	616:639	zebrafish larval rearing	616:639	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	1	49	theme	environmental	272:284	arg1	ingredients					295:305	more sustainable, cost-effective, and environmental friendly ingredients	234:305	more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards	234:348	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	5	50	theme	larvae	997:1002	arg1	application					975:985	a possible application	964:985	a possible application of insect larvae for zebrafish larval rearing	964:1031	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	5	51	theme	liver	921:925	arg1	composition					942:952	liver macromolecular composition	921:952	liver macromolecular composition	921:952	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	5	52	theme	possible	966:973	arg1	application					975:985	a possible application	964:985	a possible application of insect larvae for zebrafish larval rearing	964:1031	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	4	53	theme	major	684:688	arg1	responses					701:709	the major biological responses	680:709	the major biological responses of fish to the new diets	680:734	Through a multidisciplinary approach, the major biological responses of fish to the new diets were assessed.					
29589997	6	54	theme	insect	1110:1115	arg1	species					1117:1123	this insect species	1105:1123	this insect species	1105:1123	However, further studies are necessary to better understand the use of this insect species in the rearing of fish.					
29589997	5	55	theme	gut	899:901	arg1	inflammation					903:914	gut inflammation	899:914	gut inflammation	899:914	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	5	56	theme	lipid	858:862	arg1	metabolism					864:873	lipid metabolism	858:873	lipid metabolism	858:873	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	3	57	theme	zebrafish	616:624	arg1	rearing					633:639	zebrafish larval rearing	616:639	zebrafish larval rearing	616:639	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	3	58	theme	possible	570:577	arg1	application					579:589	the possible application	566:589	the possible application of a 100% insect diet in zebrafish larval rearing	566:639	The present study investigated, for the first time, the possible application of a 100% insect diet in zebrafish larval rearing.					
29589997	5	59	theme	acid	778:781	arg1	biometry					762:769	biometry	762:769	biometry	762:769	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	5	59	theme	acid	778:781	arg1	composition					783:793	fatty acid composition	772:793	fatty acid composition	772:793	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	5	60	theme	fish	828:831	arg1	growth					833:838	fish growth	828:838	fish growth	828:838	Results of biometry, fatty acid composition, expression of genes involved in fish growth, stress response, lipid metabolism, chitinolytic activity, gut inflammation, and liver macromolecular composition suggested a possible application of insect larvae for zebrafish larval rearing.					
29589997	1	61	theme	friendly	286:293	arg1	ingredients					295:305	more sustainable, cost-effective, and environmental friendly ingredients	234:305	more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards	234:348	A desirable goal of the aquaculture sector is to replace most of fish meal and fish oil with more sustainable, cost-effective, and environmental friendly ingredients ensuring fish health and welfare standards.					
29589997	0	62	dep	Rearing	0:6	arg1	Implications					127:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications	60:138	Rearing Zebrafish on Black Soldier Fly (Hermetia illucens): Biometric, Histological, Spectroscopic, Biochemical, and Molecular Implications.					
31077380	1	0	theme	food	232:235	arg1	industry					237:244	the food industry	228:244	the food industry	228:244	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	3	1	theme	field-pea	664:672	arg1	genotypes					674:682	field-pea genotypes	664:682	field-pea genotypes for both field-pea flour and isolated starch	664:727	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	8	2	theme	field	1661:1665	arg1	peas					1667:1670	field peas	1661:1670	field peas with the desired quality traits	1661:1702	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	4	3	theme	total	810:814	arg1	starch					816:821	total starch	810:821	total starch	810:821	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	3	4	theme	field-pea	693:701	arg1	flour					703:707	field-pea flour	693:707	field-pea flour	693:707	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	8	5	theme	desired	1681:1687	arg1	traits					1697:1702	the desired quality traits	1677:1702	the desired quality traits	1677:1702	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	4	6	theme	mean	882:885	arg1	size					902:905	the mean starch granule size	878:905	the mean starch granule size	878:905	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	7	theme	field	1033:1037	arg1	peas					1039:1042	the field peas	1029:1042	the field peas	1029:1042	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	6	8	theme	×	1242:1242	arg1	environment					1244:1254	Genotype × environment	1233:1254	Genotype × environment (G × E) interactions	1233:1275	Genotype × environment (G × E) interactions were observed for protein, some phenolic compounds, and some pasting characteristics.					
31077380	6	8	theme	×	1242:1242	arg1	G × E					1257:1261	G × E	1257:1261	G × E	1257:1261	Genotype × environment (G × E) interactions were observed for protein, some phenolic compounds, and some pasting characteristics.					
31077380	1	9	theme	dietary	364:370	arg1	fiber					372:376	dietary fiber	364:376	dietary fiber of pulses	364:386	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	2	10	theme	genetic	505:511	arg1	differences					513:523	genetic differences	505:523	genetic differences	505:523	In all crops, including cereals, oilseeds, and pulses, variability in chemical composition is known to exist due to genetic differences and environmental effects.					
31077380	4	11	dep	properties	924:933	arg1	time					995:998	peak time	990:998	peak time	990:998	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	11	dep	properties	924:933	arg1	properties					924:933	rheological properties	912:933	rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature)	912:1024	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	11	dep	properties	924:933	arg1	viscosity					962:970	breakdown viscosity	952:970	breakdown viscosity	952:970	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	11	dep	properties	924:933	arg1	viscosity					979:987	final viscosity	973:987	final viscosity	973:987	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	11	dep	properties	924:933	arg1	temperature					1013:1023	pasting temperature	1005:1023	pasting temperature	1005:1023	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	11	dep	properties	924:933	arg1	viscosity					941:949	peak viscosity	936:949	peak viscosity	936:949	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	5	12	contain	had	1074:1076	arg2	effect					1092:1097	a significant effect	1078:1097	a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas	1078:1230	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	5	12	contain	had	1074:1076	arg1	environment					1057:1067	The growing environment	1045:1067	The growing environment	1045:1067	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	5	13	theme	pasting	1194:1200	arg1	characteristics					1202:1216	pasting characteristics	1194:1216	pasting characteristics	1194:1216	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	4	14	from	effect	765:770	arg1	time					995:998	peak time	990:998	peak time	990:998	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	14	from	effect	765:770	arg1	viscosity					979:987	final viscosity	973:987	final viscosity	973:987	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	14	from	effect	765:770	arg1	properties					924:933	rheological properties	912:933	rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature)	912:1024	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	14	from	effect	765:770	arg1	viscosity					962:970	breakdown viscosity	952:970	breakdown viscosity	952:970	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	14	from	effect	765:770	arg1	composition					788:798	the chemical composition	775:798	the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds)	775:875	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	14	from	effect	765:770	arg1	size					902:905	the mean starch granule size	878:905	the mean starch granule size	878:905	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	14	from	effect	765:770	arg1	temperature					1013:1023	pasting temperature	1005:1023	pasting temperature	1005:1023	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	14	from	effect	765:770	arg1	viscosity					941:949	peak viscosity	936:949	peak viscosity	936:949	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	1	15	theme	pulses	131:136	arg1	inclusion					118:126	The inclusion	114:126	The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta	114:209	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	4	16	theme	rheological	912:922	arg1	time					995:998	peak time	990:998	peak time	990:998	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	16	theme	rheological	912:922	arg1	properties					924:933	rheological properties	912:933	rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature)	912:1024	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	16	theme	rheological	912:922	arg1	viscosity					962:970	breakdown viscosity	952:970	breakdown viscosity	952:970	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	16	theme	rheological	912:922	arg1	viscosity					979:987	final viscosity	973:987	final viscosity	973:987	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	16	theme	rheological	912:922	arg1	temperature					1013:1023	pasting temperature	1005:1023	pasting temperature	1005:1023	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	16	theme	rheological	912:922	arg1	viscosity					941:949	peak viscosity	936:949	peak viscosity	936:949	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	17	contain	had	747:749	arg2	effect					765:770	a significant effect	751:770	a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas	751:1042	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	17	contain	had	747:749	arg1	Genotype					738:745	RESULTS Genotype	730:745	RESULTS Genotype	730:745	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	1	18	theme	high	308:311	arg1	protein					313:319	high protein	308:319	high protein	308:319	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	19	theme	traditional	141:151	arg1	cakes					194:198	cakes	194:198	cakes	194:198	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	19	theme	traditional	141:151	arg1	products					170:177	traditional wheat-based food products	141:177	traditional wheat-based food products such as bread, cakes, and pasta	141:209	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	19	theme	traditional	141:151	arg1	bread					187:191	bread	187:191	bread	187:191	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	19	theme	traditional	141:151	arg1	pasta					205:209	pasta	205:209	pasta	205:209	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	5	20	theme	phenolic	1170:1177	arg1	compounds					1179:1187	some phenolic compounds	1165:1187	some phenolic compounds	1165:1187	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	5	21	theme	growing	1049:1055	arg1	environment					1057:1067	The growing environment	1045:1067	The growing environment	1045:1067	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	1	22	theme	food	165:168	arg1	cakes					194:198	cakes	194:198	cakes	194:198	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	22	theme	food	165:168	arg1	products					170:177	traditional wheat-based food products	141:177	traditional wheat-based food products such as bread, cakes, and pasta	141:209	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	22	theme	food	165:168	arg1	bread					187:191	bread	187:191	bread	187:191	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	22	theme	food	165:168	arg1	pasta					205:209	pasta	205:209	pasta	205:209	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	7	23	theme	field	1498:1502	arg1	pea					1504:1506	the field pea	1494:1506	the field pea	1494:1506	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	4	24	theme	granule	894:900	arg1	size					902:905	the mean starch granule size	878:905	the mean starch granule size	878:905	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	0	25	theme	field	91:95	arg1	peas					97:100	field peas	91:100	field peas	91:100	Genotype and environment effects on the chemical composition and rheological properties of field peas.					
31077380	2	26	theme	chemical	459:466	arg1	composition					468:478	chemical composition	459:478	chemical composition	459:478	In all crops, including cereals, oilseeds, and pulses, variability in chemical composition is known to exist due to genetic differences and environmental effects.					
31077380	3	27	from	effect	575:580	arg1	properties					650:659	the rheological properties	634:659	the rheological properties of field-pea genotypes for both field-pea flour and isolated starch	634:727	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	3	27	from	effect	575:580	arg1	composition					618:628	seed composition	613:628	seed composition	613:628	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	5	28	theme	peas	1227:1230	arg1	effect					1092:1097	a significant effect	1078:1097	a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas	1078:1230	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	5	29	theme	starch	1102:1107	arg1	size					1117:1120	starch granule size	1102:1120	starch granule size	1102:1120	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	3	30	theme	environment	598:608	arg1	effect					575:580	the effect	571:580	the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch	571:727	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	1	31	theme	nutritional	280:290	arg1	benefits					292:299	the nutritional benefits	276:299	the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses	276:386	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	7	32	theme	chemical	1443:1450	arg1	composition					1452:1462	chemical composition	1443:1462	chemical composition	1443:1462	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	4	33	theme	breakdown	952:960	arg1	properties					924:933	rheological properties	912:933	rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature)	912:1024	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	33	theme	breakdown	952:960	arg1	viscosity					962:970	breakdown viscosity	952:970	breakdown viscosity	952:970	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	3	34	theme	genotype	585:592	arg1	effect					575:580	the effect	571:580	the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch	571:727	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	6	35	theme	phenolic	1309:1316	arg1	compounds					1318:1326	some phenolic compounds	1304:1326	some phenolic compounds	1304:1326	Genotype × environment (G × E) interactions were observed for protein, some phenolic compounds, and some pasting characteristics.					
31077380	1	36	theme	due	301:303	arg1	benefits					292:299	the nutritional benefits	276:299	the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses	276:386	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	4	37	theme	pasting	1005:1011	arg1	properties					924:933	rheological properties	912:933	rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature)	912:1024	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	37	theme	pasting	1005:1011	arg1	temperature					1013:1023	pasting temperature	1005:1023	pasting temperature	1005:1023	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	7	38	theme	significant	1417:1427	arg1	effect					1429:1434	a significant effect	1415:1434	a significant effect	1415:1434	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	3	39	theme	seed	613:616	arg1	composition					618:628	seed composition	613:628	seed composition	613:628	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	4	40	theme	peak	990:993	arg1	time					995:998	peak time	990:998	peak time	990:998	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	40	theme	peak	990:993	arg1	properties					924:933	rheological properties	912:933	rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature)	912:1024	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	8	41	theme	optimized	1605:1613	arg1	practices					1625:1633	optimized agronomic practices	1605:1633	optimized agronomic practices	1605:1633	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	7	42	theme	growing	1391:1397	arg1	environment					1399:1409	the growing environment	1387:1409	the growing environment	1387:1409	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	3	43	theme	rheological	638:648	arg1	properties					650:659	the rheological properties	634:659	the rheological properties of field-pea genotypes for both field-pea flour and isolated starch	634:727	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	5	44	theme	phytic	1123:1128	arg1	acid					1130:1133	phytic acid	1123:1133	phytic acid	1123:1133	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	2	45	theme	environmental	529:541	arg1	effects					543:549	environmental effects	529:549	environmental effects	529:549	In all crops, including cereals, oilseeds, and pulses, variability in chemical composition is known to exist due to genetic differences and environmental effects.					
31077380	8	46	from	variability	1513:1523	arg1	composition					1528:1538	composition	1528:1538	composition	1528:1538	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	8	46	from	variability	1513:1523	arg1	traits					1552:1557	quality traits	1544:1557	quality traits	1544:1557	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	0	47	theme	chemical	40:47	arg1	composition					49:59	chemical composition	40:59	chemical composition	40:59	Genotype and environment effects on the chemical composition and rheological properties of field peas.					
31077380	7	48	theme	CONCLUSION	1363:1372	arg1	Genotype					1374:1381	CONCLUSION Genotype	1363:1381	CONCLUSION Genotype	1363:1381	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	3	49	theme	genotypes	674:682	arg1	properties					650:659	the rheological properties	634:659	the rheological properties of field-pea genotypes for both field-pea flour and isolated starch	634:727	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	3	49	theme	genotypes	674:682	arg1	composition					618:628	seed composition	613:628	seed composition	613:628	This study reports the effect of genotype and environment on seed composition and the rheological properties of field-pea genotypes for both field-pea flour and isolated starch.					
31077380	5	50	from	effect	1092:1097	arg1	compounds					1179:1187	some phenolic compounds	1165:1187	some phenolic compounds	1165:1187	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	5	50	from	effect	1092:1097	arg1	characteristics					1202:1216	pasting characteristics	1194:1216	pasting characteristics	1194:1216	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	5	50	from	effect	1092:1097	arg1	size					1117:1120	starch granule size	1102:1120	starch granule size	1102:1120	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	5	50	from	effect	1092:1097	arg1	acid					1130:1133	phytic acid	1123:1133	phytic acid	1123:1133	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	5	50	from	effect	1092:1097	arg1	carbohydrates					1150:1162	water-soluble carbohydrates	1136:1162	water-soluble carbohydrates	1136:1162	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	8	51	theme	peas	1667:1670	arg1	production					1647:1656	production	1647:1656	production of field peas with the desired quality traits	1647:1702	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	4	52	dep	composition	788:798	arg1	compounds					866:874	phenolic compounds	857:874	phenolic compounds	857:874	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	52	dep	composition	788:798	arg1	protein					801:807	protein	801:807	protein	801:807	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	52	dep	composition	788:798	arg1	starch					816:821	total starch	810:821	total starch	810:821	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	52	dep	composition	788:798	arg1	carbohydrates					838:850	water-soluble carbohydrates	824:850	water-soluble carbohydrates	824:850	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	7	53	contain	had	1411:1413	arg1	environment					1399:1409	the growing environment	1387:1409	the growing environment	1387:1409	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	7	53	contain	had	1411:1413	arg1	Genotype					1374:1381	CONCLUSION Genotype	1363:1381	CONCLUSION Genotype	1363:1381	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	7	53	contain	had	1411:1413	arg2	effect					1429:1434	a significant effect	1415:1434	a significant effect	1415:1434	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	4	54	theme	peas	1039:1042	arg1	effect					765:770	a significant effect	751:770	a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas	751:1042	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	6	55	theme	environment	1244:1254	arg1	interactions					1264:1275	Genotype × environment (G × E) interactions	1233:1275	Genotype × environment (G × E) interactions	1233:1275	Genotype × environment (G × E) interactions were observed for protein, some phenolic compounds, and some pasting characteristics.					
31077380	2	56	from	variability	444:454	arg1	composition					468:478	chemical composition	459:478	chemical composition	459:478	In all crops, including cereals, oilseeds, and pulses, variability in chemical composition is known to exist due to genetic differences and environmental effects.					
31077380	6	57	theme	Genotype	1233:1240	arg1	environment					1244:1254	Genotype × environment	1233:1254	Genotype × environment (G × E) interactions	1233:1275	Genotype × environment (G × E) interactions were observed for protein, some phenolic compounds, and some pasting characteristics.					
31077380	6	57	theme	Genotype	1233:1240	arg1	G × E					1257:1261	G × E	1257:1261	G × E	1257:1261	Genotype × environment (G × E) interactions were observed for protein, some phenolic compounds, and some pasting characteristics.					
31077380	1	58	theme	fiber	372:376	arg1	sources					353:359	good sources	348:359	good sources of dietary fiber of pulses	348:386	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	58	theme	fiber	372:376	arg1	protein					313:319	high protein	308:319	high protein	308:319	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	58	theme	fiber	372:376	arg1	activity					334:341	antioxidant activity	322:341	antioxidant activity	322:341	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	8	59	theme	quality	1689:1695	arg1	traits					1697:1702	the desired quality traits	1677:1702	the desired quality traits	1677:1702	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	5	60	theme	water-soluble	1136:1148	arg1	carbohydrates					1150:1162	water-soluble carbohydrates	1136:1162	water-soluble carbohydrates	1136:1162	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	1	61	theme	pulses	381:386	arg1	fiber					372:376	dietary fiber	364:376	dietary fiber of pulses	364:386	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	4	62	theme	phenolic	857:864	arg1	compounds					866:874	phenolic compounds	857:874	phenolic compounds	857:874	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	63	theme	water-soluble	824:836	arg1	carbohydrates					838:850	water-soluble carbohydrates	824:850	water-soluble carbohydrates	824:850	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	0	64	theme	rheological	65:75	arg1	properties					77:86	rheological properties	65:86	rheological properties	65:86	Genotype and environment effects on the chemical composition and rheological properties of field peas.					
31077380	7	65	theme	pea	1504:1506	arg1	composition					1452:1462	chemical composition	1443:1462	chemical composition	1443:1462	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	7	65	theme	pea	1504:1506	arg1	properties					1480:1489	rheological properties	1468:1489	rheological properties	1468:1489	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	0	66	from	effects	25:31	arg1	properties					77:86	rheological properties	65:86	rheological properties	65:86	Genotype and environment effects on the chemical composition and rheological properties of field peas.					
31077380	0	66	from	effects	25:31	arg1	composition					49:59	chemical composition	40:59	chemical composition	40:59	Genotype and environment effects on the chemical composition and rheological properties of field peas.					
31077380	8	67	dep	plant	1586:1590	arg1	breeding					1592:1599	breeding	1592:1599	breeding	1592:1599	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	1	68	dep	BACKGROUND	103:112	arg1	increasing					214:223	increasing	214:223	is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses	211:386	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	69	theme	wheat-based	153:163	arg1	cakes					194:198	cakes	194:198	cakes	194:198	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	69	theme	wheat-based	153:163	arg1	products					170:177	traditional wheat-based food products	141:177	traditional wheat-based food products such as bread, cakes, and pasta	141:209	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	69	theme	wheat-based	153:163	arg1	bread					187:191	bread	187:191	bread	187:191	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	69	theme	wheat-based	153:163	arg1	pasta					205:209	pasta	205:209	pasta	205:209	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	0	70	theme	peas	97:100	arg1	properties					77:86	rheological properties	65:86	rheological properties	65:86	Genotype and environment effects on the chemical composition and rheological properties of field peas.					
31077380	0	70	theme	peas	97:100	arg1	composition					49:59	chemical composition	40:59	chemical composition	40:59	Genotype and environment effects on the chemical composition and rheological properties of field peas.					
31077380	1	71	theme	antioxidant	322:332	arg1	activity					334:341	antioxidant activity	322:341	antioxidant activity	322:341	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	4	72	theme	starch	887:892	arg1	size					902:905	the mean starch granule size	878:905	the mean starch granule size	878:905	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	9	73	theme	Chemical	1723:1730	arg1	Industry					1732:1739	Chemical Industry	1723:1739	Chemical Industry	1723:1739	© 2019 Society of Chemical Industry.					
31077380	4	74	theme	RESULTS	730:736	arg1	Genotype					738:745	RESULTS Genotype	730:745	RESULTS Genotype	730:745	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	1	75	theme	good	348:351	arg1	sources					353:359	good sources	348:359	good sources of dietary fiber of pulses	348:386	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	8	76	with	peas	1667:1670	arg1	traits					1697:1702	the desired quality traits	1677:1702	the desired quality traits	1677:1702	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	5	77	theme	field	1221:1225	arg1	peas					1227:1230	field peas	1221:1230	field peas	1221:1230	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	6	78	theme	pasting	1338:1344	arg1	characteristics					1346:1360	some pasting characteristics	1333:1360	some pasting characteristics	1333:1360	Genotype × environment (G × E) interactions were observed for protein, some phenolic compounds, and some pasting characteristics.					
31077380	1	79	from	inclusion	118:126	arg1	cakes					194:198	cakes	194:198	cakes	194:198	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	79	from	inclusion	118:126	arg1	products					170:177	traditional wheat-based food products	141:177	traditional wheat-based food products such as bread, cakes, and pasta	141:209	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	79	from	inclusion	118:126	arg1	bread					187:191	bread	187:191	bread	187:191	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	1	79	from	inclusion	118:126	arg1	pasta					205:209	pasta	205:209	pasta	205:209	BACKGROUND The inclusion of pulses in traditional wheat-based food products such as bread, cakes, and pasta is increasing as the food industry and consumers are recognizing the nutritional benefits due to high protein, antioxidant activity, and good sources of dietary fiber of pulses.					
31077380	4	80	theme	final	973:977	arg1	properties					924:933	rheological properties	912:933	rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature)	912:1024	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	80	theme	final	973:977	arg1	viscosity					979:987	final viscosity	973:987	final viscosity	973:987	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	5	81	theme	granule	1109:1115	arg1	size					1117:1120	starch granule size	1102:1120	starch granule size	1102:1120	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	7	82	theme	rheological	1468:1478	arg1	properties					1480:1489	rheological properties	1468:1489	rheological properties	1468:1489	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	0	83	dep	composition	49:59	arg1	the					36:38	the	36:38	the	36:38	Genotype and environment effects on the chemical composition and rheological properties of field peas.					
31077380	4	84	theme	peak	936:939	arg1	properties					924:933	rheological properties	912:933	rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature)	912:1024	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	84	theme	peak	936:939	arg1	viscosity					941:949	peak viscosity	936:949	peak viscosity	936:949	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	4	85	theme	chemical	779:786	arg1	composition					788:798	the chemical composition	775:798	the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds)	775:875	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	5	86	theme	significant	1080:1090	arg1	effect					1092:1097	a significant effect	1078:1097	a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas	1078:1230	The growing environment also had a significant effect on starch granule size, phytic acid, water-soluble carbohydrates, some phenolic compounds, and pasting characteristics of field peas.					
31077380	7	87	dep	composition	1452:1462	arg1	the					1439:1441	the	1439:1441	the	1439:1441	CONCLUSION Genotype and the growing environment had a significant effect on the chemical composition and rheological properties of the field pea.					
31077380	8	88	theme	quality	1544:1550	arg1	traits					1552:1557	quality traits	1544:1557	quality traits	1544:1557	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
31077380	4	89	theme	significant	753:763	arg1	effect					765:770	a significant effect	751:770	a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas	751:1042	RESULTS Genotype had a significant effect on the chemical composition (protein, total starch, water-soluble carbohydrates, and phenolic compounds), the mean starch granule size, and rheological properties (peak viscosity, breakdown viscosity, final viscosity, peak time, and pasting temperature) of the field peas.					
31077380	8	90	theme	agronomic	1615:1623	arg1	practices					1625:1633	optimized agronomic practices	1605:1633	optimized agronomic practices	1605:1633	The variability in composition and quality traits could be exploited through plant breeding and optimized agronomic practices to increase production of field peas with the desired quality traits.					
30307376	4	0	theme	due	717:719	arg1	bio-affinity					704:715	better bio-affinity	697:715	better bio-affinity due to the layer of hyaluronic acid hydrogel	697:760	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	4	1	theme	In	584:585	arg1	experiments					654:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments	584:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments	584:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	5	2	theme	superior	938:945	arg1	differentiation					958:972	the superior osteogenic differentiation	934:972	the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds	934:1017	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	0	3	from	delivery	12:19	arg1	PEEK					24:27	PEEK	24:27	PEEK for bioactivity and osteogenesis enhancements	24:73	Simvastatin delivery on PEEK for bioactivity and osteogenesis enhancements.					
30307376	3	4	theme	L-lactic	502:509	arg1	poly					497:500	poly	497:500	the poly(L-lactic acid)/simvastatin porous film	493:539	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	3	4	theme	L-lactic	502:509	arg1	acid					511:514	L-lactic acid	502:514	L-lactic acid	502:514	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	6	5	theme	PEEK	1035:1038	arg1	samples					1040:1046	our PEEK samples	1031:1046	our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel	1031:1112	Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.					
30307376	4	6	theme	hydrogel	753:760	arg1	layer					728:732	the layer	724:732	the layer of hyaluronic acid hydrogel	724:760	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	4	7	theme	cell	593:596	arg1	attachment					598:607	cell attachment	593:607	cell attachment	593:607	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	6	8	from	potential	1125:1133	arg1	applications					1147:1158	clinical applications	1138:1158	clinical applications	1138:1158	Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.					
30307376	6	9	theme	clinical	1138:1145	arg1	applications					1147:1158	clinical applications	1138:1158	clinical applications	1138:1158	Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.					
30307376	6	10	theme	bone	1164:1167	arg1	repair					1169:1174	bone repair	1164:1174	bone repair	1164:1174	Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.					
30307376	2	11	theme	therapy	358:364	arg1	purpose					322:328	the purpose	318:328	the purpose of bone tissue regeneration therapy	318:364	In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy.					
30307376	6	12	theme	hyaluronic	1089:1098	arg1	acid					1100:1103	hyaluronic acid	1089:1103	hyaluronic acid hydrogel	1089:1112	Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.					
30307376	3	13	theme	/simvastatin	516:527	arg1	film					536:539	the poly(L-lactic acid)/simvastatin porous film	493:539	the poly(L-lactic acid)/simvastatin porous film	493:539	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	5	14	theme	osteogenic	947:956	arg1	differentiation					958:972	the superior osteogenic differentiation	934:972	the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds	934:1017	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	5	15	theme	gene	876:879	arg1	expression					881:890	gene expression	876:890	gene expression for osteogenic potential	876:915	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	5	16	theme	phosphatase	811:821	arg1	activity					823:830	the alkaline phosphatase activity	798:830	the alkaline phosphatase activity	798:830	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	3	17	theme	porous	529:534	arg1	film					536:539	the poly(L-lactic acid)/simvastatin porous film	493:539	the poly(L-lactic acid)/simvastatin porous film	493:539	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	6	18	theme	great	1119:1123	arg1	potential					1125:1133	great potential	1119:1133	great potential in clinical applications for bone repair	1119:1174	Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.					
30307376	5	19	from	differentiation	958:972	arg1	scaffolds					1009:1017	our scaffolds	1005:1017	our scaffolds	1005:1017	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	4	20	theme	acid	748:751	arg1	hydrogel					753:760	hyaluronic acid hydrogel	737:760	hyaluronic acid hydrogel	737:760	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	1	21	theme	functional	177:186	arg1	biomimetics					188:198	functional biomimetics	177:198	functional biomimetics	177:198	A strategy developed for obtaining positive cellular responses remains to be focused in the filed of functional biomimetics.					
30307376	6	22	dep	samples	1040:1046	arg1	covered					1076:1082	covered	1076:1082	covered with hyaluronic acid hydrogel	1076:1112	Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.					
30307376	6	22	dep	samples	1040:1046	arg1	loaded					1048:1053	loaded	1048:1053	loaded with simvastatin	1048:1070	Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.					
30307376	6	23	theme	acid	1100:1103	arg1	hydrogel					1105:1112	hyaluronic acid hydrogel	1089:1112	hyaluronic acid hydrogel	1089:1112	Therefore, our PEEK samples loaded with simvastatin and covered with hyaluronic acid hydrogel hold great potential in clinical applications for bone repair.					
30307376	4	24	theme	hyaluronic	737:746	arg1	acid					748:751	hyaluronic acid	737:751	hyaluronic acid hydrogel	737:760	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	3	25	theme	PEEK	436:439	arg1	surface					441:447	PEEK surface	436:447	PEEK surface	436:447	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	3	26	theme	three-dimensional	378:394	arg1	3D					397:398	3D	397:398	3D	397:398	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	3	26	theme	three-dimensional	378:394	arg1	structure					408:416	a three-dimensional (3D) porous structure	376:416	a three-dimensional (3D) porous structure	376:416	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	3	27	theme	hyaluronic	545:554	arg1	acid					556:559	hyaluronic acid	545:559	hyaluronic acid	545:559	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	4	28	theme	cytoskeletal	629:640	arg1	observation					642:652	cytoskeletal observation	629:652	cytoskeletal observation	629:652	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	5	29	theme	MC3T3-E1	977:984	arg1	pre-osteoblasts					986:1000	MC3T3-E1 pre-osteoblasts	977:1000	MC3T3-E1 pre-osteoblasts on our scaffolds	977:1017	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	1	30	theme	positive	111:118	arg1	responses					129:137	positive cellular responses	111:137	positive cellular responses	111:137	A strategy developed for obtaining positive cellular responses remains to be focused in the filed of functional biomimetics.					
30307376	5	31	theme	calcium	833:839	arg1	deposition					849:858	calcium mineral deposition	833:858	calcium mineral deposition evaluation	833:869	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	3	32	theme	poly	497:500	arg1	film					536:539	the poly(L-lactic acid)/simvastatin porous film	493:539	the poly(L-lactic acid)/simvastatin porous film	493:539	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	2	33	theme	covered	227:233	arg1	bio-composites					282:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy.					
30307376	1	34	theme	biomimetics	188:198	arg1	filed					168:172	the filed	164:172	the filed of functional biomimetics	164:198	A strategy developed for obtaining positive cellular responses remains to be focused in the filed of functional biomimetics.					
30307376	4	35	dep	In	584:585	arg1	vitro					587:591	vitro	587:591	vitro	587:591	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	2	36	theme	hydrogel	218:225	arg1	bio-composites					282:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy.					
30307376	4	37	theme	observation	642:652	arg1	experiments					654:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments	584:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments	584:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	3	38	theme	porous	401:406	arg1	3D					397:398	3D	397:398	3D	397:398	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	3	38	theme	porous	401:406	arg1	structure					408:416	a three-dimensional (3D) porous structure	376:416	a three-dimensional (3D) porous structure	376:416	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	0	39	dep	bioactivity	33:43	arg1	enhancements					62:73	enhancements	62:73	enhancements	62:73	Simvastatin delivery on PEEK for bioactivity and osteogenesis enhancements.					
30307376	1	40	theme	cellular	120:127	arg1	responses					129:137	positive cellular responses	111:137	positive cellular responses	111:137	A strategy developed for obtaining positive cellular responses remains to be focused in the filed of functional biomimetics.					
30307376	4	41	theme	attachment	598:607	arg1	experiments					654:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments	584:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments	584:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	2	42	theme	regeneration	345:356	arg1	therapy					358:364	bone tissue regeneration therapy	333:364	bone tissue regeneration therapy	333:364	In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy.					
30307376	5	43	from	pre-osteoblasts	986:1000	arg1	scaffolds					1009:1017	our scaffolds	1005:1017	our scaffolds	1005:1017	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	5	44	theme	osteogenic	896:905	arg1	potential					907:915	osteogenic potential	896:915	osteogenic potential	896:915	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	2	45	theme	PEEK	276:279	arg1	bio-composites					282:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy.					
30307376	2	46	theme	tissue	338:343	arg1	regeneration					345:356	bone tissue regeneration	333:356	bone tissue regeneration therapy	333:364	In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy.					
30307376	5	47	theme	pre-osteoblasts	986:1000	arg1	differentiation					958:972	the superior osteogenic differentiation	934:972	the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds	934:1017	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	5	48	theme	mineral	841:847	arg1	deposition					849:858	calcium mineral deposition	833:858	calcium mineral deposition evaluation	833:869	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	2	49	theme	bone	333:336	arg1	regeneration					345:356	bone tissue regeneration	333:356	bone tissue regeneration therapy	333:364	In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy.					
30307376	3	50	dep	film	536:539	arg1	hydrogel					561:568	hydrogel	561:568	hydrogel	561:568	Briefly, a three-dimensional (3D) porous structure was fabricated on PEEK surface; then the substrate was functionalized with the poly(L-lactic acid)/simvastatin porous film and hyaluronic acid hydrogel subsequently.					
30307376	5	51	from	scaffolds	1009:1017	arg1	differentiation					958:972	the superior osteogenic differentiation	934:972	the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds	934:1017	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	2	52	theme	polyetheretherketone	254:273	arg1	bio-composites					282:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy.					
30307376	5	53	theme	deposition	849:858	arg1	evaluation					860:869	calcium mineral deposition evaluation	833:869	calcium mineral deposition evaluation	833:869	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	4	54	theme	better	697:702	arg1	bio-affinity					704:715	better bio-affinity	697:715	better bio-affinity due to the layer of hyaluronic acid hydrogel	697:760	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30307376	5	55	theme	alkaline	802:809	arg1	phosphatase					811:821	the alkaline phosphatase	798:821	the alkaline phosphatase activity	798:830	Furthermore, the alkaline phosphatase activity, calcium mineral deposition evaluation, and gene expression for osteogenic potential all exhibit that the superior osteogenic differentiation of MC3T3-E1 pre-osteoblasts on our scaffolds.					
30307376	2	56	theme	simvastatin-loaded	235:252	arg1	bio-composites					282:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites	216:295	In this study, a hydrogel covered simvastatin-loaded polyetheretherketone (PEEK) bio-composites was constructed with the purpose of bone tissue regeneration therapy.					
30307376	4	57	theme	proliferation	610:622	arg1	experiments					654:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments	584:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments	584:664	In vitro cell attachment, proliferation, and cytoskeletal observation experiments reveal that our scaffolds show better bio-affinity due to the layer of hyaluronic acid hydrogel compared with control.					
30226874	2	0	dep	=	527:527	arg1	years					475:479	≥80 years	471:479	≥80 years old	471:483	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	2	0	dep	=	527:527	arg1	grade					511:515	all degenerate Pfirrmann grade 4 or 5	486:522	grade	511:515	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	2	0	dep	=	527:527	arg1	5					522:522	5	522:522	5	522:522	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	4	1	theme	nucleus	955:961	arg1	disc					922:925	the intervertebral disc	903:925	the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus)	903:1003	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	1	theme	nucleus	955:961	arg1	pulposus					963:970	nucleus pulposus	955:970	nucleus pulposus	955:970	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	2	dep	disc	922:925	arg1	disc					922:925	the intervertebral disc	903:925	the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus)	903:1003	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	2	dep	disc	922:925	arg1	fibrosus					945:952	anterior annulus fibrosus	928:952	anterior annulus fibrosus	928:952	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	2	dep	disc	922:925	arg1	fibrosus					995:1002	posterior annulus fibrosus	977:1002	posterior annulus fibrosus	977:1002	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	2	dep	disc	922:925	arg1	pulposus					963:970	nucleus pulposus	955:970	nucleus pulposus	955:970	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	5	3	theme	relative	1126:1133	arg1	glycosaminoglycans					1107:1124	sulphated and non-sulphated glycosaminoglycans	1079:1124	sulphated and non-sulphated glycosaminoglycans relative to middle-aged	1079:1148	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	5	4	from	decrease	1056:1063	arg1	content					1068:1074	content	1068:1074	content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged	1068:1148	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	5	5	from	alterations	1170:1180	arg1	proportion					1189:1198	the proportion	1185:1198	the proportion of GAG disaccharides	1185:1219	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	9	6	theme	intervertebral	1702:1715	arg1	discs					1717:1721	the intervertebral discs	1698:1721	the intervertebral discs	1698:1721	CONCLUSIONS Results show the cranial vertebral endplate is the main vascular source for the intervertebral discs.					
30226874	2	7	dep	METHODS	249:255	arg1	Middle-aged					362:372	Middle-aged	362:372	Middle-aged	362:372	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	2	7	dep	METHODS	249:255	arg1	Old-aged					461:468	Old-aged	461:468	Old-aged	461:468	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	2	7	dep	METHODS	249:255	arg1	evaluated					321:329	evaluated	321:329	evaluated	321:329	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	2	7	dep	METHODS	249:255	arg1	separated					335:343	separated	335:343	separated into two groups	335:359	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	3	8	theme	disc	626:629	arg1	cells					631:635	disc cells	626:635	disc cells	626:635	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	2	9	theme	lumbar	276:281	arg1	discs					298:302	Twenty-eight human lumbar intervertebral discs	257:302	Twenty-eight human lumbar intervertebral discs from donors	257:314	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	1	10	theme	biochemical	167:177	arg1	alterations					179:189	molecular, nutritional and biochemical alterations	140:189	molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age	140:246	OBJECTIVE This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age.					
30226874	8	11	located	observed	1535:1542	arg1	caudal					1551:1556	the caudal	1547:1556	the caudal than cranial endplates of all discs in both groups	1547:1607	Additionally, there were changes in the bony endplate, with fewer openings observed in the caudal than cranial endplates of all discs in both groups.					
30226874	8	11	located	observed	1535:1542	arg2	openings					1526:1533	fewer openings	1520:1533	fewer openings observed in the caudal than cranial endplates of all discs in both groups	1520:1607	Additionally, there were changes in the bony endplate, with fewer openings observed in the caudal than cranial endplates of all discs in both groups.					
30226874	8	12	from	changes	1485:1491	arg1	endplate					1505:1512	the bony endplate	1496:1512	the bony endplate	1496:1512	Additionally, there were changes in the bony endplate, with fewer openings observed in the caudal than cranial endplates of all discs in both groups.					
30226874	1	13	theme	intervertebral	200:213	arg1	discs					215:219	human intervertebral discs	194:219	human intervertebral discs between middle and old age	194:246	OBJECTIVE This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age.					
30226874	3	14	theme	vertebral	661:669	arg1	endplate					671:678	the vertebral endplate	657:678	the vertebral endplate	657:678	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	5	15	theme	GAG	1203:1205	arg1	disaccharides					1207:1219	GAG disaccharides	1203:1219	GAG disaccharides	1203:1219	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	3	16	theme	cell	700:703	arg1	density					705:711	cell density	700:711	cell density	700:711	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	2	17	theme	Twenty-eight	257:268	arg1	discs					298:302	Twenty-eight human lumbar intervertebral discs	257:302	Twenty-eight human lumbar intervertebral discs from donors	257:314	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	1	18	from	alterations	179:189	arg1	discs					215:219	human intervertebral discs	194:219	human intervertebral discs between middle and old age	194:246	OBJECTIVE This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age.					
30226874	3	19	theme	extracellular	726:738	arg1	composition					740:750	disc extracellular composition	721:750	disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight)	721:842	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	9	20	theme	vertebral	1647:1655	arg1	endplate					1657:1664	the cranial vertebral endplate	1635:1664	the cranial vertebral endplate	1635:1664	CONCLUSIONS Results show the cranial vertebral endplate is the main vascular source for the intervertebral discs.					
30226874	0	21	theme	bony	65:68	arg1	plates					74:79	bony end plates	65:79	bony end plates	65:79	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	10	22	theme	Hylauronic	1724:1733	arg1	acid					1735:1738	Hylauronic acid	1724:1738	Hylauronic acid molecular weight	1724:1755	Hylauronic acid molecular weight is the same through the intervertebral disc after age of 50 years.					
30226874	1	23	theme	molecular	140:148	arg1	alterations					179:189	molecular, nutritional and biochemical alterations	140:189	molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age	140:246	OBJECTIVE This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age.					
30226874	3	24	theme	glycosaminoglycan	774:790	arg1	disaccharides					792:804	glycosaminoglycan disaccharides	774:804	glycosaminoglycan disaccharides	774:804	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	2	25	dep	=	451:451	arg1	discs					418:422	35-50 years old, relatively non-degenerate discs	375:422	35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3	375:446	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	1	26	theme	nutritional	151:161	arg1	alterations					179:189	molecular, nutritional and biochemical alterations	140:189	molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age	140:246	OBJECTIVE This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age.					
30226874	2	27	theme	degenerate	490:499	arg1	grade					511:515	all degenerate Pfirrmann grade 4 or 5	486:522	grade	511:515	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	0	28	from	Changes	0:6	arg1	plates					74:79	bony end plates	65:79	bony end plates	65:79	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	0	28	from	Changes	0:6	arg1	composition					49:59	human intervertebral disc biochemical composition	11:59	human intervertebral disc biochemical composition	11:59	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	2	29	dep	Middle-aged	362:372	arg1	=					451:451	=	451:451	=	451:451	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	0	30	theme	middle	89:94	arg1	age					104:106	middle and old age	89:106	middle and old age	89:106	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	5	31	theme	size	1254:1257	arg1	alterations					1170:1180	alterations	1170:1180	alterations in the proportion of GAG disaccharides	1170:1219	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	5	31	theme	size	1254:1257	arg1	decrease					1227:1234	a decrease	1225:1234	a decrease of collagen fiber size	1225:1257	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	0	32	theme	old	100:102	arg1	age					104:106	middle and old age	89:106	middle and old age	89:106	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	3	33	dep	quantification	756:769	arg1	weight					836:841	molecular weight	826:841	molecular weight	826:841	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	4	34	theme	annulus	937:943	arg1	disc					922:925	the intervertebral disc	903:925	the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus)	903:1003	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	34	theme	annulus	937:943	arg1	fibrosus					945:952	anterior annulus fibrosus	928:952	anterior annulus fibrosus	928:952	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	2	35	theme	old	387:389	arg1	discs					418:422	35-50 years old, relatively non-degenerate discs	375:422	35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3	375:446	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	2	36	theme	Pfirrmann	427:435	arg1	discs					418:422	35-50 years old, relatively non-degenerate discs	375:422	35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3	375:446	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	0	37	theme	human	11:15	arg1	composition					49:59	human intervertebral disc biochemical composition	11:59	human intervertebral disc biochemical composition	11:59	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	4	38	theme	intervertebral	907:920	arg1	disc					922:925	the intervertebral disc	903:925	the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus)	903:1003	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	38	theme	intervertebral	907:920	arg1	fibrosus					945:952	anterior annulus fibrosus	928:952	anterior annulus fibrosus	928:952	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	38	theme	intervertebral	907:920	arg1	fibrosus					995:1002	posterior annulus fibrosus	977:1002	posterior annulus fibrosus	977:1002	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	38	theme	intervertebral	907:920	arg1	pulposus					963:970	nucleus pulposus	955:970	nucleus pulposus	955:970	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	2	39	dep	discs	418:422	arg1	grades					437:442	grades 1-3	437:446	35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3	375:446	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	5	40	theme	collagen	1239:1246	arg1	size					1254:1257	collagen fiber size	1239:1257	collagen fiber size	1239:1257	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	0	41	theme	disc	32:35	arg1	composition					49:59	human intervertebral disc biochemical composition	11:59	human intervertebral disc biochemical composition	11:59	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	5	42	dep	RESULTS	1020:1026	arg1	were					1160:1163	were	1160:1163	were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size	1160:1257	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	5	42	dep	RESULTS	1020:1026	arg1	showed					1047:1052	showed	1047:1052	showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged	1047:1148	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	2	43	dep	Old-aged	461:468	arg1	=					527:527	=	527:527	=	527:527	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	7	44	theme	cell	1430:1433	arg1	density					1435:1441	cell density	1430:1441	cell density	1430:1441	The anterior annulus differed from the posterior annulus particularly in relation to cell density and GAG content.					
30226874	4	45	theme	annulus	987:993	arg1	disc					922:925	the intervertebral disc	903:925	the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus)	903:1003	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	45	theme	annulus	987:993	arg1	fibrosus					995:1002	posterior annulus fibrosus	977:1002	posterior annulus fibrosus	977:1002	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	2	46	dep	old	387:389	arg1	non-degenerate					403:416	non-degenerate	403:416	non-degenerate	403:416	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	1	47	theme	middle	229:234	arg1	age					244:246	middle and old age	229:246	middle and old age	229:246	OBJECTIVE This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age.					
30226874	3	48	theme	nutrient	589:596	arg1	supply					598:603	nutrient supply	589:603	nutrient supply	589:603	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	8	49	theme	cranial	1563:1569	arg1	endplates					1571:1579	cranial endplates	1563:1579	cranial endplates of all discs	1563:1592	Additionally, there were changes in the bony endplate, with fewer openings observed in the caudal than cranial endplates of all discs in both groups.					
30226874	1	50	theme	old	240:242	arg1	age					244:246	middle and old age	229:246	middle and old age	229:246	OBJECTIVE This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age.					
30226874	10	51	theme	molecular	1740:1748	arg1	same					1764:1767	same	1764:1767	same	1764:1767	Hylauronic acid molecular weight is the same through the intervertebral disc after age of 50 years.					
30226874	10	51	theme	molecular	1740:1748	arg1	weight					1750:1755	Hylauronic acid molecular weight	1724:1755	Hylauronic acid molecular weight	1724:1755	Hylauronic acid molecular weight is the same through the intervertebral disc after age of 50 years.					
30226874	5	52	theme	glycosaminoglycans	1107:1124	arg1	content					1068:1074	content	1068:1074	content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged	1068:1148	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	5	53	theme	old-aged	1032:1039	arg1	group					1041:1045	The old-aged group	1028:1045	The old-aged group	1028:1045	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	7	54	theme	posterior	1384:1392	arg1	annulus					1394:1400	the posterior annulus	1380:1400	the posterior annulus	1380:1400	The anterior annulus differed from the posterior annulus particularly in relation to cell density and GAG content.					
30226874	8	55	theme	discs	1588:1592	arg1	endplates					1571:1579	cranial endplates	1563:1579	cranial endplates of all discs	1563:1592	Additionally, there were changes in the bony endplate, with fewer openings observed in the caudal than cranial endplates of all discs in both groups.					
30226874	7	56	theme	anterior	1349:1356	arg1	annulus					1358:1364	The anterior annulus	1345:1364	The anterior annulus	1345:1364	The anterior annulus differed from the posterior annulus particularly in relation to cell density and GAG content.					
30226874	9	57	theme	main	1673:1676	arg1	source					1687:1692	the main vascular source	1669:1692	the main vascular source for the intervertebral discs	1669:1721	CONCLUSIONS Results show the cranial vertebral endplate is the main vascular source for the intervertebral discs.					
30226874	3	58	theme	disaccharides	792:804	arg1	acid					821:824	hyaluronic acid	810:824	hyaluronic acid	810:824	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	3	58	theme	disaccharides	792:804	arg1	quantification					756:769	ie quantification	753:769	ie quantification of glycosaminoglycan disaccharides	753:804	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	2	59	theme	intervertebral	283:296	arg1	discs					298:302	Twenty-eight human lumbar intervertebral discs	257:302	Twenty-eight human lumbar intervertebral discs from donors	257:314	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	3	60	theme	cells	631:635	arg1	health					616:621	the health	612:621	the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density)	612:712	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	6	61	theme	Hyaluronic	1260:1269	arg1	acid					1271:1274	Hyaluronic acid	1260:1274	Hyaluronic acid molecular weight	1260:1291	Hyaluronic acid molecular weight was around 200 kDa in all regions and ages studied.					
30226874	1	62	theme	human	194:198	arg1	discs					215:219	human intervertebral discs	194:219	human intervertebral discs between middle and old age	194:246	OBJECTIVE This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age.					
30226874	2	63	theme	Pfirrmann	501:509	arg1	grade					511:515	all degenerate Pfirrmann grade 4 or 5	486:522	grade	511:515	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	3	64	theme	cell	681:684	arg1	viability					686:694	cell viability	681:694	cell viability	681:694	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	5	65	from	decrease	1227:1234	arg1	proportion					1189:1198	the proportion	1185:1198	the proportion of GAG disaccharides	1185:1219	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	3	66	theme	endplate	671:678	arg1	viability					686:694	cell viability	681:694	cell viability	681:694	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	3	66	theme	endplate	671:678	arg1	density					705:711	cell density	700:711	cell density	700:711	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	3	66	theme	endplate	671:678	arg1	porosity					645:652	the porosity	641:652	the porosity of the vertebral endplate	641:678	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	2	67	theme	human	270:274	arg1	discs					298:302	Twenty-eight human lumbar intervertebral discs	257:302	Twenty-eight human lumbar intervertebral discs from donors	257:314	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	3	68	theme	ie	753:754	arg1	quantification					756:769	ie quantification	753:769	ie quantification of glycosaminoglycan disaccharides	753:804	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	9	69	theme	cranial	1639:1645	arg1	endplate					1657:1664	the cranial vertebral endplate	1635:1664	the cranial vertebral endplate	1635:1664	CONCLUSIONS Results show the cranial vertebral endplate is the main vascular source for the intervertebral discs.					
30226874	5	70	theme	disaccharides	1207:1219	arg1	proportion					1189:1198	the proportion	1185:1198	the proportion of GAG disaccharides	1185:1219	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	0	71	theme	end	70:72	arg1	plates					74:79	bony end plates	65:79	bony end plates	65:79	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	10	72	theme	acid	1735:1738	arg1	same					1764:1767	same	1764:1767	same	1764:1767	Hylauronic acid molecular weight is the same through the intervertebral disc after age of 50 years.					
30226874	10	72	theme	acid	1735:1738	arg1	weight					1750:1755	Hylauronic acid molecular weight	1724:1755	Hylauronic acid molecular weight	1724:1755	Hylauronic acid molecular weight is the same through the intervertebral disc after age of 50 years.					
30226874	3	73	dep	porosity	645:652	arg1	eg					638:639	eg	638:639	eg	638:639	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	2	74	from	donors	309:314	arg1	discs					298:302	Twenty-eight human lumbar intervertebral discs	257:302	Twenty-eight human lumbar intervertebral discs from donors	257:314	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	3	75	theme	disc	721:724	arg1	composition					740:750	disc extracellular composition	721:750	disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight)	721:842	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	6	76	from	kDa	1308:1310	arg1	ages					1331:1334	ages	1331:1334	ages	1331:1334	Hyaluronic acid molecular weight was around 200 kDa in all regions and ages studied.					
30226874	6	76	from	kDa	1308:1310	arg1	regions					1319:1325	regions	1319:1325	regions	1319:1325	Hyaluronic acid molecular weight was around 200 kDa in all regions and ages studied.					
30226874	3	77	dep	composition	740:750	arg1	acid					821:824	hyaluronic acid	810:824	hyaluronic acid	810:824	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	3	77	dep	composition	740:750	arg1	quantification					756:769	ie quantification	753:769	ie quantification of glycosaminoglycan disaccharides	753:804	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	9	78	theme	vascular	1678:1685	arg1	source					1687:1692	the main vascular source	1669:1692	the main vascular source for the intervertebral discs	1669:1721	CONCLUSIONS Results show the cranial vertebral endplate is the main vascular source for the intervertebral discs.					
30226874	5	79	theme	fiber	1248:1252	arg1	size					1254:1257	collagen fiber size	1239:1257	collagen fiber size	1239:1257	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	6	80	theme	molecular	1276:1284	arg1	weight					1286:1291	Hyaluronic acid molecular weight	1260:1291	Hyaluronic acid molecular weight	1260:1291	Hyaluronic acid molecular weight was around 200 kDa in all regions and ages studied.					
30226874	3	81	theme	molecular	826:834	arg1	weight					836:841	molecular weight	826:841	molecular weight	826:841	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	2	82	theme	old	481:483	arg1	years					475:479	≥80 years	471:479	≥80 years old	471:483	METHODS Twenty-eight human lumbar intervertebral discs from donors were evaluated and separated into two groups: Middle-aged (35-50 years old, relatively non-degenerate discs of Pfirrmann grades 1-3, n = 15) and Old-aged (≥80 years old, all degenerate Pfirrmann grade 4 or 5, n = 13).					
30226874	1	83	dep	OBJECTIVE	109:117	arg1	evaluates					130:138	evaluates	130:138	evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age	130:246	OBJECTIVE This study evaluates molecular, nutritional and biochemical alterations in human intervertebral discs between middle and old age.					
30226874	3	84	theme	hyaluronic	810:819	arg1	acid					821:824	hyaluronic acid	810:824	hyaluronic acid	810:824	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	4	85	theme	anterior	928:935	arg1	disc					922:925	the intervertebral disc	903:925	the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus)	903:1003	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	85	theme	anterior	928:935	arg1	fibrosus					945:952	anterior annulus fibrosus	928:952	anterior annulus fibrosus	928:952	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	9	86	theme	CONCLUSIONS	1610:1620	arg1	Results					1622:1628	CONCLUSIONS Results	1610:1628	CONCLUSIONS Results	1610:1628	CONCLUSIONS Results show the cranial vertebral endplate is the main vascular source for the intervertebral discs.					
30226874	0	87	theme	intervertebral	17:30	arg1	composition					49:59	human intervertebral disc biochemical composition	11:59	human intervertebral disc biochemical composition	11:59	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	5	88	theme	sulphated	1079:1087	arg1	glycosaminoglycans					1107:1124	sulphated and non-sulphated glycosaminoglycans	1079:1124	sulphated and non-sulphated glycosaminoglycans relative to middle-aged	1079:1148	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	6	89	dep	regions	1319:1325	arg1	all					1315:1317	all	1315:1317	all	1315:1317	Hyaluronic acid molecular weight was around 200 kDa in all regions and ages studied.					
30226874	3	90	theme	collagen	848:855	arg1	organization					857:868	collagen organization	848:868	collagen organization	848:868	Parameters which might be expected to to be related to nutrient supply and so the health of disc cells (eg the porosity of the vertebral endplate, cell viability and cell density) and to disc extracellular composition (ie quantification of glycosaminoglycan disaccharides and hyaluronic acid molecular weight) and collagen organization, were analyzed.					
30226874	9	91	dep	show	1630:1633	arg1	source					1687:1692	the main vascular source	1669:1692	the main vascular source for the intervertebral discs	1669:1721	CONCLUSIONS Results show the cranial vertebral endplate is the main vascular source for the intervertebral discs.					
30226874	0	92	theme	biochemical	37:47	arg1	composition					49:59	human intervertebral disc biochemical composition	11:59	human intervertebral disc biochemical composition	11:59	Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age.					
30226874	5	93	theme	non-sulphated	1093:1105	arg1	glycosaminoglycans					1107:1124	sulphated and non-sulphated glycosaminoglycans	1079:1124	sulphated and non-sulphated glycosaminoglycans relative to middle-aged	1079:1148	RESULTS The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size.					
30226874	6	94	theme	acid	1271:1274	arg1	weight					1286:1291	Hyaluronic acid molecular weight	1260:1291	Hyaluronic acid molecular weight	1260:1291	Hyaluronic acid molecular weight was around 200 kDa in all regions and ages studied.					
30226874	7	95	theme	GAG	1447:1449	arg1	content					1451:1457	GAG content	1447:1457	GAG content	1447:1457	The anterior annulus differed from the posterior annulus particularly in relation to cell density and GAG content.					
30226874	4	96	theme	disc	922:925	arg1	regions					892:898	Three regions	886:898	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus)	886:1003	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	8	97	theme	bony	1500:1503	arg1	endplate					1505:1512	the bony endplate	1496:1512	the bony endplate	1496:1512	Additionally, there were changes in the bony endplate, with fewer openings observed in the caudal than cranial endplates of all discs in both groups.					
30226874	4	98	theme	posterior	977:985	arg1	disc					922:925	the intervertebral disc	903:925	the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus)	903:1003	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	4	98	theme	posterior	977:985	arg1	fibrosus					995:1002	posterior annulus fibrosus	977:1002	posterior annulus fibrosus	977:1002	Three regions of the intervertebral disc (anterior annulus fibrosus, nucleus pulposus, and posterior annulus fibrosus) were examined.					
30226874	8	99	from	caudal	1551:1556	arg1	groups					1602:1607	both groups	1597:1607	both groups	1597:1607	Additionally, there were changes in the bony endplate, with fewer openings observed in the caudal than cranial endplates of all discs in both groups.					
30226874	10	100	theme	intervertebral	1781:1794	arg1	disc					1796:1799	the intervertebral disc	1777:1799	the intervertebral disc after age of 50 years	1777:1821	Hylauronic acid molecular weight is the same through the intervertebral disc after age of 50 years.					
30226874	8	101	theme	fewer	1520:1524	arg1	openings					1526:1533	fewer openings	1520:1533	fewer openings observed in the caudal than cranial endplates of all discs in both groups	1520:1607	Additionally, there were changes in the bony endplate, with fewer openings observed in the caudal than cranial endplates of all discs in both groups.					
30226874	10	102	theme	years	1817:1821	arg1	age					1807:1809	age	1807:1809	age of 50 years	1807:1821	Hylauronic acid molecular weight is the same through the intervertebral disc after age of 50 years.					
29933044	5	0	theme	due	819:821	arg1	chains					812:817	inter-α-inhibitor heavy chains	788:817	inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression	788:866	Furthermore hyaluronan is complexed with inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression and sequesters CD44-expressing macrophages.					
29933044	6	1	theme	inter-α-inhibitor	1003:1019	arg1	chains					1027:1032	inter-α-inhibitor heavy chains	1003:1032	inter-α-inhibitor heavy chains	1003:1032	We show that intranasal administration of exogenous hyaluronidase is sufficient to release inter-α-inhibitor heavy chains, reduce lung hyaluronan content and restore lung function.					
29933044	1	2	theme	viral	140:144	arg1	infections					146:155	viral infections	140:155	viral infections	140:155	Little is known about the impact of viral infections on lung matrix despite its important contribution to mechanical stability and structural support.					
29933044	4	3	from	cells	724:728	arg1	synthase					678:685	hyaluronan synthase 2	667:687	hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts	667:744	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	8	4	theme	lung	1252:1255	arg1	infection					1257:1265	severe lung infection	1245:1265	severe lung infection	1245:1265	It is therefore feasible that fibrotic changes following severe lung infection and inflammation could be overcome by targeting abnormal matrix production.					
29933044	1	5	theme	infections	146:155	arg1	impact					130:135	the impact	126:135	the impact of viral infections on lung matrix	126:170	Little is known about the impact of viral infections on lung matrix despite its important contribution to mechanical stability and structural support.					
29933044	8	6	theme	severe	1245:1250	arg1	infection					1257:1265	severe lung infection	1245:1265	severe lung infection	1245:1265	It is therefore feasible that fibrotic changes following severe lung infection and inflammation could be overcome by targeting abnormal matrix production.					
29933044	4	7	theme	lung	625:628	arg1	infection					646:654	a severe lung influenza virus infection	616:654	a severe lung influenza virus infection	616:654	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	0	8	theme	hyaluronan	82:91	arg1	synthesis					93:101	prolonged, reversible hyaluronan synthesis	60:101	prolonged, reversible hyaluronan synthesis	60:101	Defective lung function following influenza virus is due to prolonged, reversible hyaluronan synthesis.					
29933044	6	9	theme	lung	1078:1081	arg1	function					1083:1090	lung function	1078:1090	lung function	1078:1090	We show that intranasal administration of exogenous hyaluronidase is sufficient to release inter-α-inhibitor heavy chains, reduce lung hyaluronan content and restore lung function.					
29933044	1	10	from	impact	130:135	arg1	matrix					165:170	lung matrix	160:170	lung matrix	160:170	Little is known about the impact of viral infections on lung matrix despite its important contribution to mechanical stability and structural support.					
29933044	4	11	from	cells	705:709	arg1	synthase					678:685	hyaluronan synthase 2	667:687	hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts	667:744	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	4	12	theme	severe	618:623	arg1	infection					646:654	a severe lung influenza virus infection	616:654	a severe lung influenza virus infection	616:654	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	2	13	theme	matrix	274:279	arg1	composition					259:269	The composition	255:269	The composition of matrix	255:279	The composition of matrix also indirectly controls inflammation by influencing cell adhesion, migration, survival, proliferation and differentiation.					
29933044	3	14	theme	lung	450:453	arg1	matrix					469:474	the lung extracellular matrix	446:474	the lung extracellular matrix	446:474	Hyaluronan is a significant component of the lung extracellular matrix and production and degradation must be carefully balanced.					
29933044	5	15	theme	inter-α-inhibitor	788:804	arg1	chains					812:817	inter-α-inhibitor heavy chains	788:817	inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression	788:866	Furthermore hyaluronan is complexed with inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression and sequesters CD44-expressing macrophages.					
29933044	6	16	theme	hyaluronidase	964:976	arg1	administration					936:949	intranasal administration	925:949	intranasal administration of exogenous hyaluronidase	925:976	We show that intranasal administration of exogenous hyaluronidase is sufficient to release inter-α-inhibitor heavy chains, reduce lung hyaluronan content and restore lung function.					
29933044	5	17	theme	elevated	826:833	arg1	expression					857:866	elevated TNF-stimulated gene 6 expression	826:866	elevated TNF-stimulated gene 6 expression	826:866	Furthermore hyaluronan is complexed with inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression and sequesters CD44-expressing macrophages.					
29933044	1	18	theme	lung	160:163	arg1	matrix					165:170	lung matrix	160:170	lung matrix	160:170	Little is known about the impact of viral infections on lung matrix despite its important contribution to mechanical stability and structural support.					
29933044	0	19	theme	lung	10:13	arg1	function					15:22	Defective lung function	0:22	Defective lung function following influenza virus	0:48	Defective lung function following influenza virus is due to prolonged, reversible hyaluronan synthesis.					
29933044	6	20	theme	exogenous	954:962	arg1	hyaluronidase					964:976	exogenous hyaluronidase	954:976	exogenous hyaluronidase	954:976	We show that intranasal administration of exogenous hyaluronidase is sufficient to release inter-α-inhibitor heavy chains, reduce lung hyaluronan content and restore lung function.					
29933044	5	21	theme	heavy	806:810	arg1	chains					812:817	inter-α-inhibitor heavy chains	788:817	inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression	788:866	Furthermore hyaluronan is complexed with inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression and sequesters CD44-expressing macrophages.					
29933044	0	22	theme	Defective	0:8	arg1	function					15:22	Defective lung function	0:22	Defective lung function following influenza virus	0:48	Defective lung function following influenza virus is due to prolonged, reversible hyaluronan synthesis.					
29933044	7	23	used	used	1118:1121	arg2	Hyaluronidase					1093:1105	Hyaluronidase	1093:1105	Hyaluronidase	1093:1105	Hyaluronidase is already used to facilitate dispersion of co-injected materials in the clinic.					
29933044	2	24	dep	cell	334:337	arg1	adhesion					339:346	adhesion	339:346	adhesion	339:346	The composition of matrix also indirectly controls inflammation by influencing cell adhesion, migration, survival, proliferation and differentiation.					
29933044	4	25	theme	epithelial	694:703	arg1	cells					705:709	epithelial cells	694:709	epithelial cells	694:709	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	8	26	theme	abnormal	1315:1322	arg1	production					1331:1340	abnormal matrix production	1315:1340	abnormal matrix production	1315:1340	It is therefore feasible that fibrotic changes following severe lung infection and inflammation could be overcome by targeting abnormal matrix production.					
29933044	4	27	theme	hyaluronan	570:579	arg1	production					581:590	hyaluronan production	570:590	hyaluronan production following resolution of a severe lung influenza virus infection	570:654	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	0	28	dep	prolonged	60:68	arg1	reversible					71:80	reversible	71:80	reversible	71:80	Defective lung function following influenza virus is due to prolonged, reversible hyaluronan synthesis.					
29933044	5	29	theme	CD44-expressing	883:897	arg1	macrophages					899:909	CD44-expressing macrophages	883:909	CD44-expressing macrophages	883:909	Furthermore hyaluronan is complexed with inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression and sequesters CD44-expressing macrophages.					
29933044	1	30	theme	important	184:192	arg1	contribution					194:205	its important contribution	180:205	its important contribution to mechanical stability and structural support	180:252	Little is known about the impact of viral infections on lung matrix despite its important contribution to mechanical stability and structural support.					
29933044	3	31	theme	significant	421:431	arg1	Hyaluronan					405:414	Hyaluronan	405:414	Hyaluronan	405:414	Hyaluronan is a significant component of the lung extracellular matrix and production and degradation must be carefully balanced.					
29933044	3	31	theme	significant	421:431	arg1	component					433:441	a significant component	419:441	a significant component of the lung extracellular matrix	419:474	Hyaluronan is a significant component of the lung extracellular matrix and production and degradation must be carefully balanced.					
29933044	6	32	theme	hyaluronan	1047:1056	arg1	content					1058:1064	lung hyaluronan content	1042:1064	lung hyaluronan content	1042:1064	We show that intranasal administration of exogenous hyaluronidase is sufficient to release inter-α-inhibitor heavy chains, reduce lung hyaluronan content and restore lung function.					
29933044	7	33	theme	materials	1163:1171	arg1	dispersion					1137:1146	dispersion	1137:1146	dispersion of co-injected materials	1137:1171	Hyaluronidase is already used to facilitate dispersion of co-injected materials in the clinic.					
29933044	4	34	from	fibroblasts	734:744	arg1	synthase					678:685	hyaluronan synthase 2	667:687	hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts	667:744	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	3	35	theme	matrix	469:474	arg1	Hyaluronan					405:414	Hyaluronan	405:414	Hyaluronan	405:414	Hyaluronan is a significant component of the lung extracellular matrix and production and degradation must be carefully balanced.					
29933044	3	35	theme	matrix	469:474	arg1	component					433:441	a significant component	419:441	a significant component of the lung extracellular matrix	419:474	Hyaluronan is a significant component of the lung extracellular matrix and production and degradation must be carefully balanced.					
29933044	0	36	theme	influenza	34:42	arg1	virus					44:48	influenza virus	34:48	influenza virus	34:48	Defective lung function following influenza virus is due to prolonged, reversible hyaluronan synthesis.					
29933044	6	37	theme	lung	1042:1045	arg1	content					1058:1064	lung hyaluronan content	1042:1064	lung hyaluronan content	1042:1064	We show that intranasal administration of exogenous hyaluronidase is sufficient to release inter-α-inhibitor heavy chains, reduce lung hyaluronan content and restore lung function.					
29933044	4	38	from	imbalance	557:565	arg1	production					581:590	hyaluronan production	570:590	hyaluronan production following resolution of a severe lung influenza virus infection	570:654	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	6	39	theme	intranasal	925:934	arg1	administration					936:949	intranasal administration	925:949	intranasal administration of exogenous hyaluronidase	925:976	We show that intranasal administration of exogenous hyaluronidase is sufficient to release inter-α-inhibitor heavy chains, reduce lung hyaluronan content and restore lung function.					
29933044	4	40	theme	hyaluronan	667:676	arg1	synthase					678:685	hyaluronan synthase 2	667:687	hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts	667:744	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	5	41	theme	gene	850:853	arg1	expression					857:866	elevated TNF-stimulated gene 6 expression	826:866	elevated TNF-stimulated gene 6 expression	826:866	Furthermore hyaluronan is complexed with inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression and sequesters CD44-expressing macrophages.					
29933044	8	42	theme	matrix	1324:1329	arg1	production					1331:1340	abnormal matrix production	1315:1340	abnormal matrix production	1315:1340	It is therefore feasible that fibrotic changes following severe lung infection and inflammation could be overcome by targeting abnormal matrix production.					
29933044	7	43	theme	co-injected	1151:1161	arg1	materials					1163:1171	co-injected materials	1151:1171	co-injected materials	1151:1171	Hyaluronidase is already used to facilitate dispersion of co-injected materials in the clinic.					
29933044	5	44	theme	TNF-stimulated	835:848	arg1	expression					857:866	elevated TNF-stimulated gene 6 expression	826:866	elevated TNF-stimulated gene 6 expression	826:866	Furthermore hyaluronan is complexed with inter-α-inhibitor heavy chains due to elevated TNF-stimulated gene 6 expression and sequesters CD44-expressing macrophages.					
29933044	4	45	theme	virus	640:644	arg1	infection					646:654	a severe lung influenza virus infection	616:654	a severe lung influenza virus infection	616:654	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	8	46	theme	fibrotic	1218:1225	arg1	changes					1227:1233	fibrotic changes	1218:1233	fibrotic changes following severe lung infection and inflammation	1218:1282	It is therefore feasible that fibrotic changes following severe lung infection and inflammation could be overcome by targeting abnormal matrix production.					
29933044	4	47	theme	influenza	630:638	arg1	infection					646:654	a severe lung influenza virus infection	616:654	a severe lung influenza virus infection	616:654	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	0	48	theme	prolonged	60:68	arg1	synthesis					93:101	prolonged, reversible hyaluronan synthesis	60:101	prolonged, reversible hyaluronan synthesis	60:101	Defective lung function following influenza virus is due to prolonged, reversible hyaluronan synthesis.					
29933044	4	49	theme	endothelial	712:722	arg1	cells					724:728	endothelial cells	712:728	endothelial cells	712:728	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	3	50	theme	extracellular	455:467	arg1	matrix					469:474	the lung extracellular matrix	446:474	the lung extracellular matrix	446:474	Hyaluronan is a significant component of the lung extracellular matrix and production and degradation must be carefully balanced.					
29933044	6	51	theme	heavy	1021:1025	arg1	chains					1027:1032	inter-α-inhibitor heavy chains	1003:1032	inter-α-inhibitor heavy chains	1003:1032	We show that intranasal administration of exogenous hyaluronidase is sufficient to release inter-α-inhibitor heavy chains, reduce lung hyaluronan content and restore lung function.					
29933044	4	52	theme	infection	646:654	arg1	resolution					602:611	resolution	602:611	resolution of a severe lung influenza virus infection	602:654	We have discovered an imbalance in hyaluronan production following resolution of a severe lung influenza virus infection, driven by hyaluronan synthase 2 from epithelial cells, endothelial cells and fibroblasts.					
29933044	1	53	theme	mechanical	210:219	arg1	stability					221:229	mechanical stability	210:229	mechanical stability	210:229	Little is known about the impact of viral infections on lung matrix despite its important contribution to mechanical stability and structural support.					
29933044	1	54	theme	structural	235:244	arg1	support					246:252	structural support	235:252	structural support	235:252	Little is known about the impact of viral infections on lung matrix despite its important contribution to mechanical stability and structural support.					
30367544	5	0	theme	cell-scaffold	797:809	arg1	interactions					811:822	cell-scaffold interactions	797:822	cell-scaffold interactions	797:822	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
30367544	0	1	with	polysucrose-blended	47:65	arg1	polydioxanone					90:102	polydioxanone	90:102	polydioxanone	90:102	Modulating matrix-multicellular response using polysucrose-blended with poly-L-lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration.					
30367544	0	1	with	polysucrose-blended	47:65	arg1	poly-L-lactide					72:85	poly-L-lactide	72:85	poly-L-lactide	72:85	Modulating matrix-multicellular response using polysucrose-blended with poly-L-lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration.					
30367544	4	2	theme	L929	608:611	arg1	fibroblasts					619:629	L929 mouse fibroblasts	608:629	L929 mouse fibroblasts	608:629	Interaction with cells was assessed using L929 mouse fibroblasts and HaCaT keratinocytes separately and in co-culture.					
30367544	0	3	theme	electrospun	107:117	arg1	scaffolds					119:127	electrospun scaffolds	107:127	electrospun scaffolds	107:127	Modulating matrix-multicellular response using polysucrose-blended with poly-L-lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration.					
30367544	0	4	from	polydioxanone	90:102	arg1	scaffolds					119:127	electrospun scaffolds	107:127	electrospun scaffolds	107:127	Modulating matrix-multicellular response using polysucrose-blended with poly-L-lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration.					
30367544	0	5	theme	skin	133:136	arg1	regeneration					145:156	skin tissue regeneration	133:156	skin tissue regeneration	133:156	Modulating matrix-multicellular response using polysucrose-blended with poly-L-lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration.					
30367544	10	6	theme	study	1466:1470	arg1	period					1456:1461	the period	1452:1461	the period of study	1452:1470	The mats maintained their physical integrity during the period of study.					
30367544	5	7	theme	parameters	695:704	arg1	Effect					685:690	Effect	685:690	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions	685:822	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
30367544	6	8	from	periphery	1095:1103	arg1	projections					1024:1034	fibrillar or fiber-like projections	1000:1034	fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells	1000:1122	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	8	from	periphery	1095:1103	arg1	lamellipodia					975:986	lamellipodia	975:986	lamellipodia	975:986	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	12	9	dep	106A	1531:1534	arg1	3275-3291					1537:1545	3275-3291	1537:1545	3275-3291	1537:1545	J Biomed Mater Res Part A: 106A: 3275-3291, 2018.					
30367544	1	10	dep	hydrophilic	181:191	arg1	has					194:196	has	194:196	has excellent biocompatibility with cells as a density gradient	194:256	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	1	10	dep	hydrophilic	181:191	arg1	resistant					265:273	resistant	265:273	resistant	265:273	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	6	11	from	nm	1058:1059	arg1	diameter					1064:1071	diameter	1064:1071	diameter	1064:1071	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	12	theme	lamellipodia	975:986	arg1	formation					962:970	the formation	958:970	the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells	958:1122	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	2	13	theme	electrospun	298:308	arg1	mats					310:313	electrospun mats	298:313	electrospun mats	298:313	Its use in electrospun mats for tissue engineering applications has not been investigated due to its amorphous nature.					
30367544	4	14	theme	mouse	613:617	arg1	fibroblasts					619:629	L929 mouse fibroblasts	608:629	L929 mouse fibroblasts	608:629	Interaction with cells was assessed using L929 mouse fibroblasts and HaCaT keratinocytes separately and in co-culture.					
30367544	0	15	from	poly-L-lactide	72:85	arg1	scaffolds					119:127	electrospun scaffolds	107:127	electrospun scaffolds	107:127	Modulating matrix-multicellular response using polysucrose-blended with poly-L-lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration.					
30367544	7	16	theme	Granular	1125:1132	arg1	matrix					1148:1153	Granular extracellular matrix	1125:1153	Granular extracellular matrix	1125:1153	Granular extracellular matrix was formed on both PLLA-PSuc and PDX-PSuc 50-50 seeded with keratinocytes.					
30367544	6	17	from	center	1108:1113	arg1	projections					1024:1034	fibrillar or fiber-like projections	1000:1034	fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells	1000:1122	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	17	from	center	1108:1113	arg1	lamellipodia					975:986	lamellipodia	975:986	lamellipodia	975:986	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	3	18	theme	nanofibrous	548:558	arg1	mats					560:563	nanofibrous mats	548:563	nanofibrous mats	548:563	For spinnability and robustness, polysucrose was blended with poly-L-lactide (PLLA) and polydioxanone (PDX) respectively and electrospun into nanofibrous mats.					
30367544	0	19	theme	tissue	138:143	arg1	regeneration					145:156	skin tissue regeneration	133:156	skin tissue regeneration	133:156	Modulating matrix-multicellular response using polysucrose-blended with poly-L-lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration.					
30367544	6	20	theme	round	923:927	arg1	morphologies					934:945	dendritic, spindle or round cell morphologies	901:945	morphologies	934:945	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	21	theme	nm	1043:1044	arg1	projections					1024:1034	fibrillar or fiber-like projections	1000:1034	fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells	1000:1122	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	21	theme	nm	1043:1044	arg1	lamellipodia					975:986	lamellipodia	975:986	lamellipodia	975:986	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	1	22	theme	excellent	198:206	arg1	biocompatibility					208:223	excellent biocompatibility	198:223	excellent biocompatibility with cells	198:234	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	1	22	theme	excellent	198:206	arg1	gradient					249:256	a density gradient	239:256	a density gradient	239:256	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	0	23	theme	matrix-multicellular	11:30	arg1	response					32:39	matrix-multicellular response	11:39	matrix-multicellular response	11:39	Modulating matrix-multicellular response using polysucrose-blended with poly-L-lactide or polydioxanone in electrospun scaffolds for skin tissue regeneration.					
30367544	6	24	from	nm	1043:1044	arg1	diameter					1064:1071	diameter	1064:1071	diameter	1064:1071	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	25	from	diameter	1064:1071	arg1	projections					1024:1034	fibrillar or fiber-like projections	1000:1034	fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells	1000:1122	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	25	from	diameter	1064:1071	arg1	lamellipodia					975:986	lamellipodia	975:986	lamellipodia	975:986	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	4	26	with	Interaction	566:576	arg1	cells					583:587	cells	583:587	cells	583:587	Interaction with cells was assessed using L929 mouse fibroblasts and HaCaT keratinocytes separately and in co-culture.					
30367544	6	27	theme	cell	929:932	arg1	morphologies					934:945	dendritic, spindle or round cell morphologies	901:945	morphologies	934:945	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	28	theme	fiber-like	1013:1022	arg1	projections					1024:1034	fibrillar or fiber-like projections	1000:1034	fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells	1000:1122	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	28	theme	fiber-like	1013:1022	arg1	lamellipodia					975:986	lamellipodia	975:986	lamellipodia	975:986	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	2	29	theme	engineering	326:336	arg1	applications					338:349	tissue engineering applications	319:349	tissue engineering applications	319:349	Its use in electrospun mats for tissue engineering applications has not been investigated due to its amorphous nature.					
30367544	7	30	dep	PLLA-PSuc	1174:1182	arg1	50-50					1197:1201	50-50	1197:1201	50-50	1197:1201	Granular extracellular matrix was formed on both PLLA-PSuc and PDX-PSuc 50-50 seeded with keratinocytes.					
30367544	8	31	theme	keratinocytes	1240:1252	arg1	Growth					1230:1235	Growth	1230:1235	Growth of keratinocytes	1230:1252	Growth of keratinocytes was enhanced in co-culture with fibroblasts with the formation of a skin-like layer.					
30367544	9	32	theme	multilayer	1377:1386	arg1	structures					1388:1397	multilayer structures	1377:1397	multilayer structures	1377:1397	Both cells showed the ability to form multilayer structures.					
30367544	10	33	theme	physical	1426:1433	arg1	integrity					1435:1443	their physical integrity	1420:1443	their physical integrity	1420:1443	The mats maintained their physical integrity during the period of study.					
30367544	2	34	theme	tissue	319:324	arg1	applications					338:349	tissue engineering applications	319:349	tissue engineering applications	319:349	Its use in electrospun mats for tissue engineering applications has not been investigated due to its amorphous nature.					
30367544	7	35	theme	extracellular	1134:1146	arg1	matrix					1148:1153	Granular extracellular matrix	1125:1153	Granular extracellular matrix	1125:1153	Granular extracellular matrix was formed on both PLLA-PSuc and PDX-PSuc 50-50 seeded with keratinocytes.					
30367544	6	36	dep	periphery	1095:1103	arg1	the					1091:1093	the	1091:1093	the	1091:1093	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	37	theme	fibrillar	1000:1008	arg1	projections					1024:1034	fibrillar or fiber-like projections	1000:1034	fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells	1000:1122	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	37	theme	fibrillar	1000:1008	arg1	lamellipodia					975:986	lamellipodia	975:986	lamellipodia	975:986	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	38	theme	spindle	912:918	arg1	morphologies					934:945	dendritic, spindle or round cell morphologies	901:945	morphologies	934:945	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	39	theme	cells	1118:1122	arg1	center					1108:1113	center	1108:1113	center	1108:1113	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	39	theme	cells	1118:1122	arg1	periphery					1095:1103	periphery	1095:1103	periphery	1095:1103	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	5	40	theme	surface	740:746	arg1	wettability					748:758	surface wettability	740:758	surface wettability	740:758	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
30367544	8	41	theme	skin-like	1322:1330	arg1	layer					1332:1336	a skin-like layer	1320:1336	a skin-like layer	1320:1336	Growth of keratinocytes was enhanced in co-culture with fibroblasts with the formation of a skin-like layer.					
30367544	6	42	theme	dendritic	901:909	arg1	morphologies					934:945	dendritic, spindle or round cell morphologies	901:945	morphologies	934:945	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	11	43	dep	©	1473:1473	arg1	Inc.					1499:1502	Inc.	1499:1502	Inc.	1499:1502	© 2018 Wiley Periodicals, Inc.					
30367544	1	44	theme	density	241:247	arg1	biocompatibility					208:223	excellent biocompatibility	198:223	excellent biocompatibility with cells	198:234	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	1	44	theme	density	241:247	arg1	gradient					249:256	a density gradient	239:256	a density gradient	239:256	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	1	45	with	biocompatibility	208:223	arg1	cells					230:234	cells	230:234	cells	230:234	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	1	46	contain	has	194:196	arg2	gradient					249:256	a density gradient	239:256	a density gradient	239:256	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	1	46	contain	has	194:196	arg1	PSuc					172:175	PSuc	172:175	PSuc	172:175	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	1	46	contain	has	194:196	arg1	Polysucrose					159:169	Polysucrose	159:169	Polysucrose (PSuc)	159:176	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	1	46	contain	has	194:196	arg2	biocompatibility					208:223	excellent biocompatibility	198:223	excellent biocompatibility with cells	198:234	Polysucrose (PSuc) is hydrophilic, has excellent biocompatibility with cells as a density gradient and is resistant to enzymes.					
30367544	5	47	from	Effect	685:690	arg1	interactions					811:822	cell-scaffold interactions	797:822	cell-scaffold interactions	797:822	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
30367544	6	48	from	projections	1024:1034	arg1	diameter					1064:1071	diameter	1064:1071	diameter	1064:1071	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	8	49	theme	layer	1332:1336	arg1	formation					1307:1315	the formation	1303:1315	the formation of a skin-like layer	1303:1336	Growth of keratinocytes was enhanced in co-culture with fibroblasts with the formation of a skin-like layer.					
30367544	2	50	from	use	291:293	arg1	mats					310:313	electrospun mats	298:313	electrospun mats	298:313	Its use in electrospun mats for tissue engineering applications has not been investigated due to its amorphous nature.					
30367544	4	51	theme	HaCaT	635:639	arg1	keratinocytes					641:653	HaCaT keratinocytes	635:653	HaCaT keratinocytes	635:653	Interaction with cells was assessed using L929 mouse fibroblasts and HaCaT keratinocytes separately and in co-culture.					
30367544	5	52	theme	fiber	724:728	arg1	diameter					730:737	fiber diameter	724:737	fiber diameter	724:737	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
30367544	6	53	theme	mats	876:879	arg1	nature					850:855	nature	850:855	nature	850:855	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	53	theme	mats	876:879	arg1	composition					861:871	composition	861:871	composition	861:871	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	8	54	with	co-culture	1270:1279	arg1	fibroblasts					1286:1296	fibroblasts	1286:1296	fibroblasts	1286:1296	Growth of keratinocytes was enhanced in co-culture with fibroblasts with the formation of a skin-like layer.					
30367544	8	54	with	co-culture	1270:1279	arg1	formation					1307:1315	the formation	1303:1315	the formation of a skin-like layer	1303:1336	Growth of keratinocytes was enhanced in co-culture with fibroblasts with the formation of a skin-like layer.					
30367544	6	55	theme	nm	1058:1059	arg1	projections					1024:1034	fibrillar or fiber-like projections	1000:1034	fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells	1000:1122	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	6	55	theme	nm	1058:1059	arg1	lamellipodia					975:986	lamellipodia	975:986	lamellipodia	975:986	Depending on nature and composition of mats, fibroblasts showed dendritic, spindle or round cell morphologies along with the formation of lamellipodia, filopodia, fibrillar or fiber-like projections of 100 nm and 200-300 nm in diameter respectively from the periphery or center of cells.					
30367544	5	56	theme	mats	789:792	arg1	properties					775:784	mechanical properties	764:784	mechanical properties	764:784	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
30367544	5	56	theme	mats	789:792	arg1	porosity					714:721	porosity	714:721	porosity	714:721	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
30367544	5	56	theme	mats	789:792	arg1	diameter					730:737	fiber diameter	724:737	fiber diameter	724:737	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
30367544	5	56	theme	mats	789:792	arg1	wettability					748:758	surface wettability	740:758	surface wettability	740:758	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
30367544	12	57	theme	Part	1523:1526	arg1	A					1528:1528	Part A	1523:1528	J Biomed Mater Res Part A: 106A: 3275-3291, 2018.	1504:1552	J Biomed Mater Res Part A: 106A: 3275-3291, 2018.					
30367544	2	58	theme	amorphous	388:396	arg1	nature					398:403	its amorphous nature	384:403	its amorphous nature	384:403	Its use in electrospun mats for tissue engineering applications has not been investigated due to its amorphous nature.					
30367544	12	59	dep	Res	1519:1521	arg1	106A					1531:1534	106A	1531:1534	106A	1531:1534	J Biomed Mater Res Part A: 106A: 3275-3291, 2018.					
30367544	12	59	dep	Res	1519:1521	arg1	A					1528:1528	Part A	1523:1528	J Biomed Mater Res Part A: 106A: 3275-3291, 2018.	1504:1552	J Biomed Mater Res Part A: 106A: 3275-3291, 2018.					
30367544	5	60	theme	mechanical	764:773	arg1	properties					775:784	mechanical properties	764:784	mechanical properties	764:784	Effect of parameters such as porosity, fiber diameter, surface wettability and mechanical properties of mats on cell-scaffold interactions was studied.					
31161485	4	0	theme	second	764:769	arg1	CM2					792:794	CM2	792:794	CM2	792:794	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	4	0	theme	second	764:769	arg1	mixture					783:789	a second concentrate mixture	762:789	a second concentrate mixture	762:789	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	8	1	theme	fat	1383:1385	arg1	values					1368:1373	Overall mean values	1355:1373	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids	1355:1436	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	5	2	theme	respective	1031:1040	arg1	mixtures					1054:1061	their respective concentrate mixtures	1025:1061	their respective concentrate mixtures	1025:1061	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	7	3	theme	Fat-corrected	1180:1192	arg1	production					1205:1214	Fat-corrected milk (FCM) production	1180:1214	Fat-corrected milk (FCM) production	1180:1214	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	8	4	theme	solid	1388:1392	arg1	fat					1398:1400	solid not fat	1388:1400	solid not fat	1388:1400	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	6	5	theme	body	1097:1100	arg1	score					1112:1116	body condition score	1097:1116	body condition score	1097:1116	Dry matter intake, body weights, body condition score, and total milk yield were similar between groups (P > 0.05).					
31161485	8	6	theme	solids	1431:1436	arg1	values					1368:1373	Overall mean values	1355:1373	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids	1355:1436	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	4	7	theme	BPPF	741:744	arg1	groups					746:751	BPPF groups	741:751	BPPF groups	741:751	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	4	8	theme	fed	603:605	arg1	groups					607:612	Control and BPF fed groups	587:612	groups	607:612	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	9	9	with	supplementation	1549:1563	arg1	BPP					1570:1572	BPP	1570:1572	BPP	1570:1572	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	9	9	with	supplementation	1549:1563	arg1	BPF					1577:1579	BPF	1577:1579	BPF	1577:1579	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	0	10	theme	early	121:125	arg1	lactation					127:135	early lactation	121:135	early lactation	121:135	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	1	11	theme	nutrients	168:176	arg1	effect					142:147	The effect	138:147	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF)	138:243	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	4	12	theme	rumen-protected	805:819	arg1	protein					821:827	40% rumen-protected protein	801:827	40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake)	801:883	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	1	13	dep	protein	203:209	arg1	bypass					196:201	bypass	196:201	bypass	196:201	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	9	14	theme	milk	1649:1652	arg1	quality					1638:1644	the quality	1634:1644	the quality of milk produced in Murrah buffaloes	1634:1681	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	6	15	theme	Dry	1064:1066	arg1	intake					1075:1080	Dry matter intake	1064:1080	Dry matter intake	1064:1080	Dry matter intake, body weights, body condition score, and total milk yield were similar between groups (P > 0.05).					
31161485	6	16	theme	milk	1129:1132	arg1	yield					1134:1138	total milk yield	1123:1138	total milk yield	1123:1138	Dry matter intake, body weights, body condition score, and total milk yield were similar between groups (P > 0.05).					
31161485	8	17	theme	treatment	1474:1482	arg1	groups					1484:1489	treatment groups	1474:1489	treatment groups	1474:1489	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	4	18	theme	40	801:802	arg1	%					803:803	%	803:803	%	803:803	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	4	19	theme	%	657:657	arg1	protein					675:681	25% rumen-protected protein	655:681	25% rumen-protected protein	655:681	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	3	20	from	day	511:513	arg1	ration					499:504	ration	499:504	ration from day 0 to 90 postpartum	499:532	Buffaloes individually fed ration from day 0 to 90 postpartum according to feeding group and nutrient requirement.					
31161485	3	21	theme	nutrient	565:572	arg1	requirement					574:584	nutrient requirement	565:584	nutrient requirement	565:584	Buffaloes individually fed ration from day 0 to 90 postpartum according to feeding group and nutrient requirement.					
31161485	0	22	from	yield	71:75	arg1	Murrah					97:102	Murrah	97:102	Murrah	97:102	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	9	23	theme	early	1690:1694	arg1	lactation					1696:1704	early lactation	1690:1704	early lactation	1690:1704	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	4	24	theme	concentrate	625:635	arg1	mixture					637:643	a concentrate mixture	623:643	a concentrate mixture	623:643	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	4	24	theme	concentrate	625:635	arg1	CM1					646:648	CM1	646:648	CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake)	646:730	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	4	24	theme	concentrate	625:635	arg1	BPP					733:735	BPP	733:735	BPP	733:735	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	7	25	dep	significant	1281:1291	arg1	%					1299:1299	19.45%	1294:1299	19.45%	1294:1299	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	7	25	dep	significant	1281:1291	arg1	P < 0.05					1302:1309	P < 0.05	1302:1309	P < 0.05	1302:1309	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	5	26	theme	Ca	969:970	arg1	salt					972:975	Ca salt	969:975	Ca salt of long-chain fatty acids	969:1001	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	7	27	theme	BPF-fed	1340:1346	arg1	group					1348:1352	BPF-fed group	1340:1352	BPF-fed group	1340:1352	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	7	28	dep	improved	1220:1227	arg1	P > 0.05					1237:1244	P > 0.05	1237:1244	P > 0.05	1237:1244	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	7	28	dep	improved	1220:1227	arg1	%					1234:1234	14.5%	1230:1234	14.5%	1230:1234	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	0	29	from	Effect	0:5	arg1	intake					44:49	feed intake	39:49	feed intake	39:49	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	0	29	from	Effect	0:5	arg1	weights					57:63	body weights	52:63	body weights	52:63	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	0	29	from	Effect	0:5	arg1	yield					71:75	milk yield	66:75	milk yield	66:75	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	0	29	from	Effect	0:5	arg1	composition					82:92	composition	82:92	composition	82:92	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	8	30	dep	high	1455:1458	arg1	P < 0.05					1461:1468	P < 0.05	1461:1468	P < 0.05	1461:1468	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	1	31	theme	early	293:297	arg1	stage					299:303	the early stage	289:303	the early stage of lactation	289:316	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	9	32	dep	supplementation	1549:1563	arg1	either					1581:1586	either	1581:1586	either	1581:1586	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	9	32	dep	supplementation	1549:1563	arg1	combination					1600:1610	combination	1600:1610	combination	1600:1610	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	2	33	theme	milk	455:458	arg1	production					460:469	milk production	455:469	milk production	455:469	Forty Murrah buffaloes (BW 531.92 ± 10.85 kg) just after parturition were randomly distributed into four groups according to parity and milk production.					
31161485	0	34	from	intake	44:49	arg1	Murrah					97:102	Murrah	97:102	Murrah	97:102	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	5	35	theme	kg	1008:1009	arg1	yield					1016:1020	kg milk yield	1008:1020	kg milk yield	1008:1020	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	0	36	theme	rumen-protected	10:24	arg1	nutrients					26:34	rumen-protected nutrients	10:34	rumen-protected nutrients	10:34	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	9	37	theme	milk	1772:1775	arg1	trait					1763:1767	the quantitative trait	1746:1767	the quantitative trait of milk	1746:1775	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	2	38	theme	BW	343:344	arg1	buffaloes					332:340	Forty Murrah buffaloes	319:340	Forty Murrah buffaloes (BW 531.92 ± 10.85 kg) just after parturition	319:386	Forty Murrah buffaloes (BW 531.92 ± 10.85 kg) just after parturition were randomly distributed into four groups according to parity and milk production.					
31161485	2	38	theme	BW	343:344	arg1	531.92 ± 10.85 kg					346:362	BW 531.92 ± 10.85 kg	343:362	BW 531.92 ± 10.85 kg	343:362	Forty Murrah buffaloes (BW 531.92 ± 10.85 kg) just after parturition were randomly distributed into four groups according to parity and milk production.					
31161485	5	39	theme	fatty	991:995	arg1	acids					997:1001	long-chain fatty acids	980:1001	long-chain fatty acids	980:1001	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	5	40	theme	fat	893:895	arg1	BPPF					917:920	BPPF	917:920	BPPF	917:920	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	5	40	theme	fat	893:895	arg1	BPF					909:911	BPF	909:911	BPF	909:911	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	5	40	theme	fat	893:895	arg1	groups					901:906	fat fed groups	893:906	Bypass fat fed groups (BPF and BPPF)	886:921	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	0	41	theme	body	52:55	arg1	weights					57:63	body weights	52:63	body weights	52:63	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	1	42	theme	Murrah	265:270	arg1	buffaloes					272:280	Murrah buffaloes	265:280	Murrah buffaloes	265:280	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	7	43	theme	significant	1281:1291	arg1	improvement					1312:1322	significant (19.45%, P < 0.05) improvement	1281:1322	significant (19.45%, P < 0.05) improvement	1281:1322	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	4	44	theme	de-oiled	844:851	arg1	bran					858:861	de-oiled rice bran	844:861	de-oiled rice bran	844:861	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	1	45	dep	fat	186:188	arg1	bypass					179:184	bypass	179:184	bypass	179:184	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	4	46	theme	oil	722:724	arg1	cake					726:729	mustard oil cake	714:729	mustard oil cake	714:729	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	4	47	theme	BPF	599:601	arg1	groups					607:612	Control and BPF fed groups	587:612	groups	607:612	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	9	48	from	improvement	1731:1741	arg1	trait					1763:1767	the quantitative trait	1746:1767	the quantitative trait of milk	1746:1775	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	8	49	theme	milk	1378:1381	arg1	fat					1383:1385	milk fat	1378:1385	milk fat	1378:1385	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	4	50	with	CM1	646:648	arg1	protein					675:681	25% rumen-protected protein	655:681	25% rumen-protected protein	655:681	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	6	51	theme	condition	1102:1110	arg1	score					1112:1116	body condition score	1097:1116	body condition score	1097:1116	Dry matter intake, body weights, body condition score, and total milk yield were similar between groups (P > 0.05).					
31161485	5	52	theme	concentrate	1042:1052	arg1	mixtures					1054:1061	their respective concentrate mixtures	1025:1061	their respective concentrate mixtures	1025:1061	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	4	53	theme	concentrate	771:781	arg1	CM2					792:794	CM2	792:794	CM2	792:794	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	4	53	theme	concentrate	771:781	arg1	mixture					783:789	a second concentrate mixture	762:789	a second concentrate mixture	762:789	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	8	54	theme	total	1425:1429	arg1	solids					1431:1436	total solids	1425:1436	total solids	1425:1436	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	5	55	dep	groups	901:906	arg1	BPPF					917:920	BPPF	917:920	BPPF	917:920	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	5	55	dep	groups	901:906	arg1	BPF					909:911	BPF	909:911	BPF	909:911	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	5	55	dep	groups	901:906	arg1	Bypass					886:891	Bypass	886:891	Bypass	886:891	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	5	55	dep	groups	901:906	arg1	groups					901:906	fat fed groups	893:906	Bypass fat fed groups (BPF and BPPF)	886:921	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	6	56	theme	body	1083:1086	arg1	weights					1088:1094	body weights	1083:1094	body weights	1083:1094	Dry matter intake, body weights, body condition score, and total milk yield were similar between groups (P > 0.05).					
31161485	1	57	theme	rumen-protected	152:166	arg1	nutrients					168:176	rumen-protected nutrients	152:176	rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF)	152:243	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	4	58	theme	%	803:803	arg1	protein					821:827	40% rumen-protected protein	801:827	40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake)	801:883	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	0	59	from	weights	57:63	arg1	Murrah					97:102	Murrah	97:102	Murrah	97:102	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	6	60	theme	matter	1068:1073	arg1	intake					1075:1080	Dry matter intake	1064:1080	Dry matter intake	1064:1080	Dry matter intake, body weights, body condition score, and total milk yield were similar between groups (P > 0.05).					
31161485	8	61	from	groups	1484:1489	arg1	high					1455:1458	high	1455:1458	high	1455:1458	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	0	62	from	composition	82:92	arg1	Murrah					97:102	Murrah	97:102	Murrah	97:102	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	7	63	theme	milk	1194:1197	arg1	production					1205:1214	Fat-corrected milk (FCM) production	1180:1214	Fat-corrected milk (FCM) production	1180:1214	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	9	64	theme	Murrah	1666:1671	arg1	buffaloes					1673:1681	Murrah buffaloes	1666:1681	Murrah buffaloes	1666:1681	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	6	65	theme	total	1123:1127	arg1	yield					1134:1138	total milk yield	1123:1138	total milk yield	1123:1138	Dry matter intake, body weights, body condition score, and total milk yield were similar between groups (P > 0.05).					
31161485	4	66	theme	25	655:656	arg1	%					657:657	%	657:657	%	657:657	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	9	67	contain	had	1727:1729	arg1	BPF					1710:1712	BPF	1710:1712	BPF	1710:1712	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	9	67	contain	had	1727:1729	arg2	improvement					1731:1741	improvement	1731:1741	improvement on the quantitative trait of milk	1731:1775	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	3	68	theme	feeding	547:553	arg1	group					555:559	feeding group	547:559	feeding group	547:559	Buffaloes individually fed ration from day 0 to 90 postpartum according to feeding group and nutrient requirement.					
31161485	4	69	theme	rice	853:856	arg1	bran					858:861	de-oiled rice bran	844:861	de-oiled rice bran	844:861	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	0	70	theme	milk	66:69	arg1	yield					71:75	milk yield	66:75	milk yield	66:75	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	5	71	theme	long-chain	980:989	arg1	acids					997:1001	long-chain fatty acids	980:1001	long-chain fatty acids	980:1001	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	8	72	theme	control	1508:1514	arg1	values					1516:1521	control values	1508:1521	control values	1508:1521	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	5	73	theme	15 g	959:962	arg1	BPF					964:966	15 g BPF	959:966	15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures	959:1061	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	4	74	theme	wheat	698:702	arg1	bran					704:707	wheat bran	698:707	wheat bran	698:707	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	2	75	theme	Murrah	325:330	arg1	buffaloes					332:340	Forty Murrah buffaloes	319:340	Forty Murrah buffaloes (BW 531.92 ± 10.85 kg) just after parturition	319:386	Forty Murrah buffaloes (BW 531.92 ± 10.85 kg) just after parturition were randomly distributed into four groups according to parity and milk production.					
31161485	2	75	theme	Murrah	325:330	arg1	531.92 ± 10.85 kg					346:362	BW 531.92 ± 10.85 kg	343:362	BW 531.92 ± 10.85 kg	343:362	Forty Murrah buffaloes (BW 531.92 ± 10.85 kg) just after parturition were randomly distributed into four groups according to parity and milk production.					
31161485	5	76	from	BPF	964:966	arg1	mixtures					1054:1061	their respective concentrate mixtures	1025:1061	their respective concentrate mixtures	1025:1061	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	1	77	theme	lactation	308:316	arg1	stage					299:303	the early stage	289:303	the early stage of lactation	289:316	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	9	78	theme	quantitative	1750:1761	arg1	trait					1763:1767	the quantitative trait	1746:1767	the quantitative trait of milk	1746:1775	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	4	79	theme	rumen-protected	659:673	arg1	protein					675:681	25% rumen-protected protein	655:681	25% rumen-protected protein	655:681	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	0	80	theme	nutrients	26:34	arg1	Effect					0:5	Effect	0:5	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah	0:102	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	5	81	theme	milk	1011:1014	arg1	yield					1016:1020	kg milk yield	1008:1020	kg milk yield	1008:1020	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	9	82	dep	either	1581:1586	arg1	alone					1588:1592	alone	1588:1592	alone	1588:1592	It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.					
31161485	0	83	theme	feed	39:42	arg1	intake					44:49	feed intake	39:49	feed intake	39:49	Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation.					
31161485	7	84	located	observed	1328:1335	arg1	group					1348:1352	BPF-fed group	1340:1352	BPF-fed group	1340:1352	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	7	84	located	observed	1328:1335	arg2	improvement					1312:1322	significant (19.45%, P < 0.05) improvement	1281:1322	significant (19.45%, P < 0.05) improvement	1281:1322	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	4	85	theme	cottonseed	868:877	arg1	cake					879:882	cottonseed cake	868:882	cottonseed cake	868:882	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	5	86	dep	BPF	964:966	arg1	salt					972:975	Ca salt	969:975	Ca salt of long-chain fatty acids	969:1001	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	1	87	dep	nutrients	168:176	arg1	BPF					191:193	BPF	191:193	BPF	191:193	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	1	87	dep	nutrients	168:176	arg1	protein					203:209	bypass protein	196:209	bypass protein	196:209	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	1	87	dep	nutrients	168:176	arg1	combination					226:236	their combination	220:236	their combination	220:236	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	1	87	dep	nutrients	168:176	arg1	BPPF					239:242	BPPF	239:242	BPPF	239:242	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	1	87	dep	nutrients	168:176	arg1	fat					186:188	bypass fat	179:188	bypass fat	179:188	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	1	87	dep	nutrients	168:176	arg1	BPP					212:214	BPP	212:214	BPP	212:214	The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation.					
31161485	5	88	theme	acids	997:1001	arg1	salt					972:975	Ca salt	969:975	Ca salt of long-chain fatty acids	969:1001	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	5	89	theme	fed	897:899	arg1	BPPF					917:920	BPPF	917:920	BPPF	917:920	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	5	89	theme	fed	897:899	arg1	BPF					909:911	BPF	909:911	BPF	909:911	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	5	89	theme	fed	897:899	arg1	groups					901:906	fat fed groups	893:906	Bypass fat fed groups (BPF and BPPF)	886:921	Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures.					
31161485	8	90	theme	fat	1398:1400	arg1	values					1368:1373	Overall mean values	1355:1373	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids	1355:1436	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	7	91	theme	FCM	1200:1202	arg1	production					1205:1214	Fat-corrected milk (FCM) production	1180:1214	Fat-corrected milk (FCM) production	1180:1214	Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group.					
31161485	8	92	theme	mean	1363:1366	arg1	values					1368:1373	Overall mean values	1355:1373	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids	1355:1436	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	8	93	from	high	1455:1458	arg1	groups					1484:1489	treatment groups	1474:1489	treatment groups	1474:1489	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	8	94	theme	protein	1403:1409	arg1	values					1368:1373	Overall mean values	1355:1373	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids	1355:1436	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
31161485	4	95	theme	Control	587:593	arg1	groups					607:612	Control and BPF fed groups	587:612	groups	607:612	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	4	96	theme	mustard	714:720	arg1	cake					726:729	mustard oil cake	714:729	mustard oil cake	714:729	Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake).					
31161485	8	97	theme	lactose	1412:1418	arg1	values					1368:1373	Overall mean values	1355:1373	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids	1355:1436	Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values.					
30692173	10	0	theme	ecology	1901:1907	arg1	understanding					1876:1888	our understanding	1872:1888	our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis	1872:1952	These findings have a strong impact on our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis.					
30692173	10	1	theme	pathogenesis	1941:1952	arg1	understanding					1876:1888	our understanding	1872:1888	our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis	1872:1952	These findings have a strong impact on our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis.					
30692173	9	2	theme	lipooligosaccharide	1654:1672	arg1	synthesis					1630:1638	the synthesis	1626:1638	the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1626:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	3	3	theme	cstV	679:682	arg1	role					661:664	the biological role	646:664	the biological role of cstIV and cstV	646:682	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	3	theme	cstV	679:682	arg1	unknown					687:693	unknown	687:693	unknown	687:693	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	2	4	theme	triggering	288:297	arg1	mimicry					234:240	Ganglioside mimicry	222:240	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS)	222:279	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	2	4	theme	triggering	288:297	arg1	factor					299:304	the triggering factor	284:304	the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy	284:362	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	9	5	theme	Campylobacter	1677:1689	arg1	jejuni					1691:1696	Campylobacter jejuni	1677:1696	Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1677:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	6	from	jejuni	1691:1696	arg1	synthesis					1630:1638	the synthesis	1626:1638	the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1626:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	5	7	dep	in	967:968	arg1	vitro					970:974	vitro	970:974	vitro	970:974	Attempts were made to identify donor and acceptor molecules using in vitro activity tests with recombinant GT-42 enzymes.					
30692173	2	8	theme	syndrome	324:331	arg1	mimicry					234:240	Ganglioside mimicry	222:240	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS)	222:279	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	2	8	theme	syndrome	324:331	arg1	factor					299:304	the triggering factor	284:304	the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy	284:362	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	9	9	theme	present	1705:1711	arg1	study					1713:1717	the present study	1701:1717	the present study	1701:1717	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	8	10	theme	C.	1386:1387	arg1	locus					1427:1431	the C. coli lipooligosaccharide biosynthesis locus	1382:1431	the C. coli lipooligosaccharide biosynthesis locus	1382:1431	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus was largely unknown until recently.					
30692173	8	11	theme	lipooligosaccharide	1394:1412	arg1	locus					1427:1431	the C. coli lipooligosaccharide biosynthesis locus	1382:1431	the C. coli lipooligosaccharide biosynthesis locus	1382:1431	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus was largely unknown until recently.					
30692173	5	12	theme	GT-42	1008:1012	arg1	enzymes					1014:1020	recombinant GT-42 enzymes	996:1020	recombinant GT-42 enzymes	996:1020	Attempts were made to identify donor and acceptor molecules using in vitro activity tests with recombinant GT-42 enzymes.					
30692173	2	13	theme	C.	245:246	arg1	jejuni					248:253	C. jejuni lipooligosaccharide (LOS)	245:279	C. jejuni lipooligosaccharide (LOS)	245:279	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	9	14	from	synthesis	1630:1638	arg1	jejuni					1691:1696	Campylobacter jejuni	1677:1696	Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1677:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	4	15	theme	chemical	872:879	arg1	composition					881:891	the chemical composition	868:891	the chemical composition of LOS	868:898	In the present investigation, mutation studies with two strains expressing either cstIV or cstV were performed and mass spectrometry was used to investigate differences in the chemical composition of LOS.					
30692173	8	16	theme	locus	1427:1431	arg1	makeup					1372:1377	The genetic makeup	1360:1377	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus	1360:1431	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus was largely unknown until recently.					
30692173	8	16	theme	locus	1427:1431	arg1	unknown					1445:1451	unknown	1445:1451	unknown	1445:1451	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus was largely unknown until recently.					
30692173	7	17	theme	major	1289:1293	arg1	pathogen					1305:1312	a major foodborne pathogen	1287:1312	Campylobacter coli a major foodborne pathogen	1268:1312	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	9	18	theme	several	1576:1582	arg1	glycosyltransferases					1593:1612	several putative glycosyltransferases	1576:1612	several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1576:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	1	19	theme	bacterial	182:190	arg1	gastroenteritis					192:206	bacterial gastroenteritis	182:206	bacterial gastroenteritis	182:206	Campylobacter jejuni and Campylobacter coli are the most common causes of bacterial gastroenteritis in the world.					
30692173	4	20	theme	mutation	726:733	arg1	studies					735:741	mutation studies	726:741	mutation studies with two strains expressing either cstIV or cstV	726:790	In the present investigation, mutation studies with two strains expressing either cstIV or cstV were performed and mass spectrometry was used to investigate differences in the chemical composition of LOS.					
30692173	3	21	from	family	409:414	arg1	Sialyltransferases					365:382	Sialyltransferases	365:382	Sialyltransferases from glycosyltransferase family 42 (GT-42)	365:425	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	9	22	located	found	1732:1736	arg1	study					1713:1717	the present study	1701:1717	the present study	1701:1717	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	22	located	found	1732:1736	arg2	coli					1723:1726	C. coli	1720:1726	C. coli	1720:1726	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	23	theme	sialic	1791:1796	arg1	acid					1798:1801	sialic acid	1791:1801	sialic acid	1791:1801	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	7	24	theme	LOS	1137:1139	arg1	sialylation					1141:1151	LOS sialylation	1137:1151	LOS sialylation	1137:1151	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	5	25	theme	acceptor	942:949	arg1	molecules					951:959	donor and acceptor molecules	932:959	donor and acceptor molecules using in vitro activity tests with recombinant GT-42 enzymes	932:1020	Attempts were made to identify donor and acceptor molecules using in vitro activity tests with recombinant GT-42 enzymes.					
30692173	9	26	theme	lipooligosaccharide	1522:1540	arg1	biosynthesis					1542:1553	lipooligosaccharide biosynthesis	1522:1553	lipooligosaccharide biosynthesis	1522:1553	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	27	theme	nonulosonate	1813:1824	arg1	acids					1826:1830	other nonulosonate acids	1807:1830	other nonulosonate acids	1807:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	3	28	theme	C.	485:486	arg1	jejuni					488:493	C. jejuni	485:493	C. jejuni	485:493	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	29	from	present	590:596	arg1	%					604:604	∼11%	601:604	∼11% of currently available C. coli genomes	601:643	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	29	from	present	590:596	arg1	genomes					637:643	currently available C. coli genomes	609:643	currently available C. coli genomes	609:643	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	9	30	dep	jejuni	1691:1696	arg1	found					1732:1736	found	1732:1736	was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1728:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	7	31	dep	Campylobacter	1268:1280	arg1	pathogen					1305:1312	a major foodborne pathogen	1287:1312	Campylobacter coli a major foodborne pathogen	1268:1312	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	7	31	dep	Campylobacter	1268:1280	arg1	coli					1282:1285	Campylobacter coli a major foodborne pathogen	1268:1312	Campylobacter coli a major foodborne pathogen	1268:1312	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	3	32	theme	available	619:627	arg1	genomes					637:643	currently available C. coli genomes	609:643	currently available C. coli genomes	609:643	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	0	33	theme	Acid-Containing	48:62	arg1	Lipooligosaccharides					64:83	Nonulosonic Acid-Containing Lipooligosaccharides	36:83	Nonulosonic Acid-Containing Lipooligosaccharides	36:83	Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli.					
30692173	3	34	dep	ganglioside	463:473	arg1	mimics					475:480	mimics	475:480	mimics	475:480	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	6	35	theme	coli	1075:1078	arg1	biosynthesis					1084:1095	C. coli LOS biosynthesis	1072:1095	C. coli LOS biosynthesis	1072:1095	Here we show that CstIV and CstV are involved in C. coli LOS biosynthesis.					
30692173	3	36	theme	biological	650:659	arg1	role					661:664	the biological role	646:664	the biological role of cstIV and cstV	646:682	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	36	theme	biological	650:659	arg1	unknown					687:693	unknown	687:693	unknown	687:693	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	7	37	theme	diacetylated	1197:1208	arg1	residue.IMPORTANCE					1227:1244	a diacetylated nonulosonic acid residue.IMPORTANCE	1195:1244	a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected	1195:1357	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	3	38	from	expression	449:458	arg1	jejuni					488:493	C. jejuni	485:493	C. jejuni	485:493	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	39	dep	C.	629:630	arg1	coli					632:635	coli	632:635	coli	632:635	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	7	40	theme	acid	1222:1225	arg1	residue.IMPORTANCE					1227:1244	a diacetylated nonulosonic acid residue.IMPORTANCE	1195:1244	a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected	1195:1357	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	3	41	theme	GT-42	515:519	arg1	genes					521:525	two novel GT-42 genes	505:525	two novel GT-42 genes	505:525	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	41	theme	GT-42	515:519	arg1	cstV					538:541	cstV	538:541	cstV	538:541	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	41	theme	GT-42	515:519	arg1	cstIV					528:532	cstIV	528:532	cstIV	528:532	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	10	42	theme	coli	1896:1899	arg1	ecology					1901:1907	C. coli ecology	1893:1907	C. coli ecology	1893:1907	These findings have a strong impact on our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis.					
30692173	2	43	theme	Guillain-Barré	309:322	arg1	polyneuropathy					349:362	an acute polyneuropathy	340:362	an acute polyneuropathy	340:362	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	2	43	theme	Guillain-Barré	309:322	arg1	GBS					334:336	GBS	334:336	GBS	334:336	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	2	43	theme	Guillain-Barré	309:322	arg1	syndrome					324:331	Guillain-Barré syndrome	309:331	Guillain-Barré syndrome (GBS)	309:337	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	9	44	theme	sialylated	1643:1652	arg1	lipooligosaccharide					1654:1672	sialylated lipooligosaccharide	1643:1672	sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1643:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	6	45	theme	LOS	1080:1082	arg1	biosynthesis					1084:1095	C. coli LOS biosynthesis	1072:1095	C. coli LOS biosynthesis	1072:1095	Here we show that CstIV and CstV are involved in C. coli LOS biosynthesis.					
30692173	1	46	theme	gastroenteritis	192:206	arg1	causes					172:177	the most common causes	156:177	the most common causes of bacterial gastroenteritis in the world	156:219	Campylobacter jejuni and Campylobacter coli are the most common causes of bacterial gastroenteritis in the world.					
30692173	1	46	theme	gastroenteritis	192:206	arg1	Campylobacter					133:145	Campylobacter	133:145	Campylobacter	133:145	Campylobacter jejuni and Campylobacter coli are the most common causes of bacterial gastroenteritis in the world.					
30692173	1	46	theme	gastroenteritis	192:206	arg1	jejuni					122:127	jejuni	122:127	jejuni	122:127	Campylobacter jejuni and Campylobacter coli are the most common causes of bacterial gastroenteritis in the world.					
30692173	10	47	theme	interaction	1924:1934	arg1	understanding					1876:1888	our understanding	1872:1888	our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis	1872:1952	These findings have a strong impact on our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis.					
30692173	4	48	theme	mass	811:814	arg1	spectrometry					816:827	mass spectrometry	811:827	mass spectrometry	811:827	In the present investigation, mutation studies with two strains expressing either cstIV or cstV were performed and mass spectrometry was used to investigate differences in the chemical composition of LOS.					
30692173	4	49	with	studies	735:741	arg1	strains					752:758	two strains	748:758	two strains expressing either cstIV or cstV	748:790	In the present investigation, mutation studies with two strains expressing either cstIV or cstV were performed and mass spectrometry was used to investigate differences in the chemical composition of LOS.					
30692173	5	50	theme	recombinant	996:1006	arg1	enzymes					1014:1020	recombinant GT-42 enzymes	996:1020	recombinant GT-42 enzymes	996:1020	Attempts were made to identify donor and acceptor molecules using in vitro activity tests with recombinant GT-42 enzymes.					
30692173	9	51	contain	containing	1780:1789	arg2	acid					1798:1801	sialic acid	1791:1801	sialic acid	1791:1801	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	51	contain	containing	1780:1789	arg2	acids					1826:1830	other nonulosonate acids	1807:1830	other nonulosonate acids	1807:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	51	contain	containing	1780:1789	arg1	structures					1769:1778	lipooligosaccharide structures	1749:1778	lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1749:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	52	from	lipooligosaccharide	1654:1672	arg1	jejuni					1691:1696	Campylobacter jejuni	1677:1696	Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1677:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	53	theme	large	1487:1491	arg1	genes					1500:1504	genes	1500:1504	genes	1500:1504	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	53	theme	large	1487:1491	arg1	set					1493:1495	a large set	1485:1495	a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1485:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	53	theme	large	1487:1491	arg1	genes					1566:1570	genes	1566:1570	genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1566:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	2	54	theme	acute	343:347	arg1	polyneuropathy					349:362	an acute polyneuropathy	340:362	an acute polyneuropathy	340:362	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	2	54	theme	acute	343:347	arg1	syndrome					324:331	Guillain-Barré syndrome	309:331	Guillain-Barré syndrome (GBS)	309:337	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	4	55	theme	LOS	896:898	arg1	composition					881:891	the chemical composition	868:891	the chemical composition of LOS	868:898	In the present investigation, mutation studies with two strains expressing either cstIV or cstV were performed and mass spectrometry was used to investigate differences in the chemical composition of LOS.					
30692173	8	56	theme	biosynthesis	1414:1425	arg1	locus					1427:1431	the C. coli lipooligosaccharide biosynthesis locus	1382:1431	the C. coli lipooligosaccharide biosynthesis locus	1382:1431	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus was largely unknown until recently.					
30692173	7	57	dep	residue.IMPORTANCE	1227:1244	arg1	neglected					1349:1357	neglected	1349:1357	has been largely neglected	1332:1357	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	7	58	attach	linked	1169:1174	arg1	addition					1183:1190	the addition	1179:1190	the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected	1179:1357	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	7	58	attach	linked	1169:1174	arg2	cstIV					1160:1164	cstIV	1160:1164	cstIV	1160:1164	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	4	59	from	differences	853:863	arg1	composition					881:891	the chemical composition	868:891	the chemical composition of LOS	868:898	In the present investigation, mutation studies with two strains expressing either cstIV or cstV were performed and mass spectrometry was used to investigate differences in the chemical composition of LOS.					
30692173	2	60	dep	jejuni	248:253	arg1	lipooligosaccharide					255:273	lipooligosaccharide	255:273	C. jejuni lipooligosaccharide (LOS)	245:279	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	2	60	dep	jejuni	248:253	arg1	LOS					276:278	LOS	276:278	LOS	276:278	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	1	61	theme	common	165:170	arg1	causes					172:177	the most common causes	156:177	the most common causes of bacterial gastroenteritis in the world	156:219	Campylobacter jejuni and Campylobacter coli are the most common causes of bacterial gastroenteritis in the world.					
30692173	1	61	theme	common	165:170	arg1	Campylobacter					133:145	Campylobacter	133:145	Campylobacter	133:145	Campylobacter jejuni and Campylobacter coli are the most common causes of bacterial gastroenteritis in the world.					
30692173	1	61	theme	common	165:170	arg1	jejuni					122:127	jejuni	122:127	jejuni	122:127	Campylobacter jejuni and Campylobacter coli are the most common causes of bacterial gastroenteritis in the world.					
30692173	0	62	from	Biosynthesis	20:31	arg1	Campylobacter					88:100	Campylobacter	88:100	Campylobacter	88:100	Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli.					
30692173	7	63	theme	foodborne	1295:1303	arg1	pathogen					1305:1312	a major foodborne pathogen	1287:1312	Campylobacter coli a major foodborne pathogen	1268:1312	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	1	64	dep	Campylobacter	133:145	arg1	coli					147:150	Campylobacter coli	133:150	Campylobacter coli	133:150	Campylobacter jejuni and Campylobacter coli are the most common causes of bacterial gastroenteritis in the world.					
30692173	9	65	theme	lipooligosaccharide	1749:1767	arg1	structures					1769:1778	lipooligosaccharide structures	1749:1778	lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1749:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	2	66	theme	Ganglioside	222:232	arg1	mimicry					234:240	Ganglioside mimicry	222:240	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS)	222:279	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	2	66	theme	Ganglioside	222:232	arg1	factor					299:304	the triggering factor	284:304	the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy	284:362	Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy.					
30692173	3	67	theme	glycosyltransferase	389:407	arg1	family					409:414	glycosyltransferase family 42	389:417	glycosyltransferase family 42 (GT-42)	389:425	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	67	theme	glycosyltransferase	389:407	arg1	GT-42					420:424	GT-42	420:424	GT-42	420:424	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	5	68	theme	donor	932:936	arg1	molecules					951:959	donor and acceptor molecules	932:959	donor and acceptor molecules using in vitro activity tests with recombinant GT-42 enzymes	932:1020	Attempts were made to identify donor and acceptor molecules using in vitro activity tests with recombinant GT-42 enzymes.					
30692173	1	69	from	causes	172:177	arg1	world					215:219	the world	211:219	the world	211:219	Campylobacter jejuni and Campylobacter coli are the most common causes of bacterial gastroenteritis in the world.					
30692173	9	70	theme	genes	1500:1504	arg1	genes					1500:1504	genes	1500:1504	genes	1500:1504	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	70	theme	genes	1500:1504	arg1	set					1493:1495	a large set	1485:1495	a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1485:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	9	70	theme	genes	1500:1504	arg1	genes					1566:1570	genes	1566:1570	genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1566:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	3	71	from	%	604:604	arg1	present					590:596	present	590:596	present	590:596	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	72	theme	ganglioside	463:473	arg1	expression					449:458	the expression	445:458	the expression of ganglioside mimics in C. jejuni	445:493	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	9	73	theme	other	1807:1811	arg1	acids					1826:1830	other nonulosonate acids	1807:1830	other nonulosonate acids	1807:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	0	74	dep	Campylobacter	88:100	arg1	coli					102:105	Campylobacter coli	88:105	Campylobacter coli	88:105	Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli.					
30692173	3	75	theme	C.	629:630	arg1	genomes					637:643	currently available C. coli genomes	609:643	currently available C. coli genomes	609:643	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	4	76	theme	present	703:709	arg1	investigation					711:723	the present investigation	699:723	the present investigation	699:723	In the present investigation, mutation studies with two strains expressing either cstIV or cstV were performed and mass spectrometry was used to investigate differences in the chemical composition of LOS.					
30692173	0	77	theme	Nonulosonic	36:46	arg1	Lipooligosaccharides					64:83	Nonulosonic Acid-Containing Lipooligosaccharides	36:83	Nonulosonic Acid-Containing Lipooligosaccharides	36:83	Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli.					
30692173	4	78	used	used	833:836	arg2	spectrometry					816:827	mass spectrometry	811:827	mass spectrometry	811:827	In the present investigation, mutation studies with two strains expressing either cstIV or cstV were performed and mass spectrometry was used to investigate differences in the chemical composition of LOS.					
30692173	5	79	theme	activity	976:983	arg1	tests					985:989	in vitro activity tests	967:989	in vitro activity tests	967:989	Attempts were made to identify donor and acceptor molecules using in vitro activity tests with recombinant GT-42 enzymes.					
30692173	7	80	theme	residue.IMPORTANCE	1227:1244	arg1	addition					1183:1190	the addition	1179:1190	the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected	1179:1357	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	10	81	theme	strong	1855:1860	arg1	impact					1862:1867	a strong impact	1853:1867	a strong impact	1853:1867	These findings have a strong impact on our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis.					
30692173	0	82	theme	Lipooligosaccharides	64:83	arg1	Biosynthesis					20:31	the Biosynthesis	16:31	the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli	16:105	Genetics behind the Biosynthesis of Nonulosonic Acid-Containing Lipooligosaccharides in Campylobacter coli.					
30692173	7	83	theme	nonulosonic	1210:1220	arg1	residue.IMPORTANCE					1227:1244	a diacetylated nonulosonic acid residue.IMPORTANCE	1195:1244	a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected	1195:1357	In particular, cstV is associated with LOS sialylation, while cstIV is linked to the addition of a diacetylated nonulosonic acid residue.IMPORTANCE Despite the fact that Campylobacter coli a major foodborne pathogen, its glycobiology has been largely neglected.					
30692173	10	84	contain	have	1848:1851	arg1	findings					1839:1846	These findings	1833:1846	These findings	1833:1846	These findings have a strong impact on our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis.					
30692173	10	84	contain	have	1848:1851	arg2	impact					1862:1867	a strong impact	1853:1867	a strong impact	1853:1867	These findings have a strong impact on our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis.					
30692173	10	85	theme	host-pathogen	1910:1922	arg1	interaction					1924:1934	host-pathogen interaction	1910:1934	host-pathogen interaction	1910:1934	These findings have a strong impact on our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis.					
30692173	10	86	theme	C.	1893:1894	arg1	ecology					1901:1907	C. coli ecology	1893:1907	C. coli ecology	1893:1907	These findings have a strong impact on our understanding of C. coli ecology, host-pathogen interaction, and pathogenesis.					
30692173	3	87	theme	novel	509:513	arg1	genes					521:525	two novel GT-42 genes	505:525	two novel GT-42 genes	505:525	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	87	theme	novel	509:513	arg1	cstV					538:541	cstV	538:541	cstV	538:541	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	87	theme	novel	509:513	arg1	cstIV					528:532	cstIV	528:532	cstIV	528:532	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	8	88	dep	C.	1386:1387	arg1	coli					1389:1392	coli	1389:1392	coli	1389:1392	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus was largely unknown until recently.					
30692173	9	89	theme	putative	1584:1591	arg1	glycosyltransferases					1593:1612	several putative glycosyltransferases	1576:1612	several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1576:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	6	90	theme	C.	1072:1073	arg1	biosynthesis					1084:1095	C. coli LOS biosynthesis	1072:1095	C. coli LOS biosynthesis	1072:1095	Here we show that CstIV and CstV are involved in C. coli LOS biosynthesis.					
30692173	3	91	theme	genomes	637:643	arg1	%					604:604	∼11%	601:604	∼11% of currently available C. coli genomes	601:643	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	91	theme	genomes	637:643	arg1	genomes					637:643	currently available C. coli genomes	609:643	currently available C. coli genomes	609:643	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	8	92	theme	genetic	1364:1370	arg1	makeup					1372:1377	The genetic makeup	1360:1377	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus	1360:1431	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus was largely unknown until recently.					
30692173	8	92	theme	genetic	1364:1370	arg1	unknown					1445:1451	unknown	1445:1451	unknown	1445:1451	The genetic makeup of the C. coli lipooligosaccharide biosynthesis locus was largely unknown until recently.					
30692173	9	93	gly	sialylated	1643:1652	arg1	lipooligosaccharide					1654:1672	sialylated lipooligosaccharide	1643:1672	sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids	1643:1830	C. coli harbors a large set of genes associated with lipooligosaccharide biosynthesis, including genes for several putative glycosyltransferases involved in the synthesis of sialylated lipooligosaccharide in Campylobacter jejuni In the present study, C. coli was found to express lipooligosaccharide structures containing sialic acid and other nonulosonate acids.					
30692173	5	94	theme	in	967:968	arg1	tests					985:989	in vitro activity tests	967:989	in vitro activity tests	967:989	Attempts were made to identify donor and acceptor molecules using in vitro activity tests with recombinant GT-42 enzymes.					
30692173	3	95	theme	cstIV	669:673	arg1	role					661:664	the biological role	646:664	the biological role of cstIV and cstV	646:682	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
30692173	3	95	theme	cstIV	669:673	arg1	unknown					687:693	unknown	687:693	unknown	687:693	Sialyltransferases from glycosyltransferase family 42 (GT-42) are essential for the expression of ganglioside mimics in C. jejuni Recently, two novel GT-42 genes, cstIV and cstV, have been identified in C. coli Despite being present in ∼11% of currently available C. coli genomes, the biological role of cstIV and cstV is unknown.					
31196537	2	0	theme	antimicrobial	328:340	arg1	properties					342:351	antimicrobial properties	328:351	antimicrobial properties	328:351	In this thesis, we introduce a new technique to functionalize bacterial cellulose (BC) with antimicrobial properties by in situ method.					
31196537	3	1	theme	purifying	481:489	arg1	methods					455:461	the methods	451:461	the methods of fermenting and purifying to obtain a BC complex with antimicrobial properties	451:542	We design a series of experiments on hydrolyzing mulberry leaves and exploring the methods of fermenting and purifying to obtain a BC complex with antimicrobial properties.					
31196537	2	2	theme	new	267:269	arg1	technique					271:279	a new technique	265:279	a new technique to functionalize bacterial cellulose (BC) with antimicrobial properties by in situ method	265:369	In this thesis, we introduce a new technique to functionalize bacterial cellulose (BC) with antimicrobial properties by in situ method.					
31196537	0	3	theme	mulberry	95:102	arg1	bioconversion					78:90	bioconversion	78:90	bioconversion of mulberry	78:102	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry leaves.					
31196537	4	4	theme	experimental	741:752	arg1	results					754:760	the experimental results	737:760	the experimental results	737:760	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	4	5	theme	anti-bacterial	560:573	arg1	performances					575:586	the anti-bacterial performances	556:586	the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium)	556:667	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	4	5	theme	anti-bacterial	560:573	arg1	Meanwhile					545:553	Meanwhile	545:553	Meanwhile	545:553	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	5	6	theme	excellent	842:850	arg1	biocompatibility					852:867	the excellent biocompatibility	838:867	the excellent biocompatibility of MH-BC towards hMSCs	838:890	Considering the excellent biocompatibility of MH-BC towards hMSCs, we expect that this antibacterial functional BC composite will find potential applications in biomedicine and regenerative medicine.					
31196537	4	7	theme	hydrolysate	636:646	arg1	medium					661:666	acid hydrolysate fermentation medium	631:666	acid hydrolysate fermentation medium	631:666	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	3	8	theme	BC	503:504	arg1	complex					506:512	a BC complex	501:512	a BC complex with antimicrobial properties	501:542	We design a series of experiments on hydrolyzing mulberry leaves and exploring the methods of fermenting and purifying to obtain a BC complex with antimicrobial properties.					
31196537	4	9	theme	acid	631:634	arg1	medium					661:666	acid hydrolysate fermentation medium	631:666	acid hydrolysate fermentation medium	631:666	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	2	10	theme	bacterial	298:306	arg1	BC					319:320	BC	319:320	BC	319:320	In this thesis, we introduce a new technique to functionalize bacterial cellulose (BC) with antimicrobial properties by in situ method.					
31196537	2	10	theme	bacterial	298:306	arg1	cellulose					308:316	bacterial cellulose	298:316	bacterial cellulose (BC) with antimicrobial properties	298:351	In this thesis, we introduce a new technique to functionalize bacterial cellulose (BC) with antimicrobial properties by in situ method.					
31196537	0	11	theme	In	0:1	arg1	preparation					8:18	In situ preparation	0:18	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry	0:102	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry leaves.					
31196537	5	12	theme	MH-BC	872:876	arg1	biocompatibility					852:867	the excellent biocompatibility	838:867	the excellent biocompatibility of MH-BC towards hMSCs	838:890	Considering the excellent biocompatibility of MH-BC towards hMSCs, we expect that this antibacterial functional BC composite will find potential applications in biomedicine and regenerative medicine.					
31196537	4	13	dep	mulberry	615:622	arg1	leaves					624:629	leaves	624:629	leaves acid hydrolysate fermentation medium	624:666	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	0	14	from	bioconversion	78:90	arg1	properties					62:71	antimicrobial properties	48:71	antimicrobial properties from bioconversion of mulberry	48:102	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry leaves.					
31196537	3	15	theme	antimicrobial	519:531	arg1	properties					533:542	antimicrobial properties	519:542	antimicrobial properties	519:542	We design a series of experiments on hydrolyzing mulberry leaves and exploring the methods of fermenting and purifying to obtain a BC complex with antimicrobial properties.					
31196537	1	16	theme	important	188:196	arg1	function					198:205	the most important function	179:205	the most important function in the field of biomedicine	179:233	It's long been understood that antimicrobial properties are one of the most important function in the field of biomedicine.					
31196537	4	17	theme	MH-BC	591:595	arg1	performances					575:586	the anti-bacterial performances	556:586	the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium)	556:667	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	4	17	theme	MH-BC	591:595	arg1	Meanwhile					545:553	Meanwhile	545:553	Meanwhile	545:553	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	1	18	theme	function	198:205	arg1	function					198:205	the most important function	179:205	the most important function in the field of biomedicine	179:233	It's long been understood that antimicrobial properties are one of the most important function in the field of biomedicine.					
31196537	1	18	theme	function	198:205	arg1	one					172:174	one	172:174	one	172:174	It's long been understood that antimicrobial properties are one of the most important function in the field of biomedicine.					
31196537	0	19	theme	cellulose	33:41	arg1	preparation					8:18	In situ preparation	0:18	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry	0:102	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry leaves.					
31196537	5	20	theme	functional	927:936	arg1	composite					941:949	this antibacterial functional BC composite	908:949	this antibacterial functional BC composite	908:949	Considering the excellent biocompatibility of MH-BC towards hMSCs, we expect that this antibacterial functional BC composite will find potential applications in biomedicine and regenerative medicine.					
31196537	2	21	with	cellulose	308:316	arg1	properties					342:351	antimicrobial properties	328:351	antimicrobial properties	328:351	In this thesis, we introduce a new technique to functionalize bacterial cellulose (BC) with antimicrobial properties by in situ method.					
31196537	3	22	theme	experiments	394:404	arg1	series					384:389	a series	382:389	a series of experiments	382:404	We design a series of experiments on hydrolyzing mulberry leaves and exploring the methods of fermenting and purifying to obtain a BC complex with antimicrobial properties.					
31196537	0	23	theme	bacterial	23:31	arg1	cellulose					33:41	bacterial cellulose	23:41	bacterial cellulose	23:41	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry leaves.					
31196537	4	24	theme	anti-bacterial	799:812	arg1	activities					814:823	excellent anti-bacterial activities	789:823	excellent anti-bacterial activities	789:823	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	0	25	theme	antimicrobial	48:60	arg1	properties					62:71	antimicrobial properties	48:71	antimicrobial properties from bioconversion of mulberry	48:102	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry leaves.					
31196537	2	26	theme	in	356:357	arg1	method					364:369	in situ method	356:369	in situ method	356:369	In this thesis, we introduce a new technique to functionalize bacterial cellulose (BC) with antimicrobial properties by in situ method.					
31196537	0	27	with	preparation	8:18	arg1	properties					62:71	antimicrobial properties	48:71	antimicrobial properties from bioconversion of mulberry	48:102	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry leaves.					
31196537	5	28	theme	antibacterial	913:925	arg1	composite					941:949	this antibacterial functional BC composite	908:949	this antibacterial functional BC composite	908:949	Considering the excellent biocompatibility of MH-BC towards hMSCs, we expect that this antibacterial functional BC composite will find potential applications in biomedicine and regenerative medicine.					
31196537	3	29	theme	fermenting	466:475	arg1	methods					455:461	the methods	451:461	the methods of fermenting and purifying to obtain a BC complex with antimicrobial properties	451:542	We design a series of experiments on hydrolyzing mulberry leaves and exploring the methods of fermenting and purifying to obtain a BC complex with antimicrobial properties.					
31196537	4	30	theme	fermentation	648:659	arg1	medium					661:666	acid hydrolysate fermentation medium	631:666	acid hydrolysate fermentation medium	631:666	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	3	31	dep	mulberry	421:428	arg1	leaves					430:435	leaves	430:435	leaves	430:435	We design a series of experiments on hydrolyzing mulberry leaves and exploring the methods of fermenting and purifying to obtain a BC complex with antimicrobial properties.					
31196537	1	32	from	function	198:205	arg1	field					214:218	the field	210:218	the field of biomedicine	210:233	It's long been understood that antimicrobial properties are one of the most important function in the field of biomedicine.					
31196537	5	33	theme	BC	938:939	arg1	composite					941:949	this antibacterial functional BC composite	908:949	this antibacterial functional BC composite	908:949	Considering the excellent biocompatibility of MH-BC towards hMSCs, we expect that this antibacterial functional BC composite will find potential applications in biomedicine and regenerative medicine.					
31196537	5	34	theme	potential	961:969	arg1	applications					971:982	potential applications	961:982	potential applications in biomedicine and regenerative medicine	961:1023	Considering the excellent biocompatibility of MH-BC towards hMSCs, we expect that this antibacterial functional BC composite will find potential applications in biomedicine and regenerative medicine.					
31196537	4	35	contain	have	784:787	arg2	activities					814:823	excellent anti-bacterial activities	789:823	excellent anti-bacterial activities	789:823	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	4	35	contain	have	784:787	arg1	MH-BC					778:782	MH-BC	778:782	MH-BC	778:782	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	5	36	theme	regenerative	1003:1014	arg1	medicine					1016:1023	regenerative medicine	1003:1023	regenerative medicine	1003:1023	Considering the excellent biocompatibility of MH-BC towards hMSCs, we expect that this antibacterial functional BC composite will find potential applications in biomedicine and regenerative medicine.					
31196537	2	37	dep	in	356:357	arg1	situ					359:362	situ	359:362	situ	359:362	In this thesis, we introduce a new technique to functionalize bacterial cellulose (BC) with antimicrobial properties by in situ method.					
31196537	4	38	theme	excellent	789:797	arg1	activities					814:823	excellent anti-bacterial activities	789:823	excellent anti-bacterial activities	789:823	Meanwhile, the anti-bacterial performances of MH-BC (fermented by the mulberry leaves acid hydrolysate fermentation medium) were evaluated with Escherichia coli and Staphylococcus aureus, and the experimental results showed that the MH-BC have excellent anti-bacterial activities.					
31196537	5	39	from	applications	971:982	arg1	biomedicine					987:997	biomedicine	987:997	biomedicine	987:997	Considering the excellent biocompatibility of MH-BC towards hMSCs, we expect that this antibacterial functional BC composite will find potential applications in biomedicine and regenerative medicine.					
31196537	5	39	from	applications	971:982	arg1	medicine					1016:1023	regenerative medicine	1003:1023	regenerative medicine	1003:1023	Considering the excellent biocompatibility of MH-BC towards hMSCs, we expect that this antibacterial functional BC composite will find potential applications in biomedicine and regenerative medicine.					
31196537	1	40	theme	biomedicine	223:233	arg1	field					214:218	the field	210:218	the field of biomedicine	210:233	It's long been understood that antimicrobial properties are one of the most important function in the field of biomedicine.					
31196537	3	41	with	complex	506:512	arg1	properties					533:542	antimicrobial properties	519:542	antimicrobial properties	519:542	We design a series of experiments on hydrolyzing mulberry leaves and exploring the methods of fermenting and purifying to obtain a BC complex with antimicrobial properties.					
31196537	0	42	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ preparation of bacterial cellulose with antimicrobial properties from bioconversion of mulberry leaves.					
31196537	1	43	theme	antimicrobial	143:155	arg1	properties					157:166	antimicrobial properties	143:166	antimicrobial properties	143:166	It's long been understood that antimicrobial properties are one of the most important function in the field of biomedicine.					
29396700	8	0	theme	developed	1157:1165	arg1	membranes					1167:1175	the herein developed membranes	1146:1175	the herein developed membranes	1146:1175	These results highlight the interest of the herein developed membranes for dual drug delivery.					
29396700	7	1	theme	hydrophilic	1004:1014	arg1	drugs					1016:1020	hydrophilic drugs	1004:1020	hydrophilic drugs	1004:1020	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	8	2	theme	dual	1181:1184	arg1	delivery					1191:1198	dual drug delivery	1181:1198	dual drug delivery	1181:1198	These results highlight the interest of the herein developed membranes for dual drug delivery.					
29396700	7	3	theme	Drug	912:915	arg1	studies					926:932	Drug delivery studies	912:932	Drug delivery studies	912:932	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	0	4	theme	composite	84:92	arg1	membranes					94:102	chitosan/diatomaceous earth composite membranes	56:102	chitosan/diatomaceous earth composite membranes	56:102	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.					
29396700	7	5	theme	immediate	983:991	arg1	release					993:999	the immediate release	979:999	the immediate release of hydrophilic drugs	979:1020	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	8	6	theme	drug	1186:1189	arg1	delivery					1191:1198	dual drug delivery	1181:1198	dual drug delivery	1181:1198	These results highlight the interest of the herein developed membranes for dual drug delivery.					
29396700	7	7	theme	formulations	962:973	arg1	potential					945:953	the potential	941:953	the potential of all formulations for the immediate release of hydrophilic drugs	941:1020	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	5	8	theme	concept	658:664	arg1	membranes					671:679	the membranes	667:679	the membranes	667:679	As proof of concept, the membranes were loaded with a hydrophilic (gentamicin) and a hydrophobic (dexamethasone) drug.					
29396700	5	8	theme	concept	658:664	arg1	proof					649:653	proof	649:653	proof of concept	649:664	As proof of concept, the membranes were loaded with a hydrophilic (gentamicin) and a hydrophobic (dexamethasone) drug.					
29396700	2	9	dep	compromised	355:365	arg1	cancer…					390:396	cancer…	390:396	cancer…	390:396	For instance, hepatic metabolism means an important drug loss, while some patients have their ability to swell highly compromised (i.e. unconsciousness, cancer…).					
29396700	4	10	theme	marine	594:599	arg1	sediments					601:609	marine sediments	594:609	marine sediments for dual sublingual drug delivery	594:643	This work explores the use of the β-chitosan membranes, from marine industry residues, composed with marine sediments for dual sublingual drug delivery.					
29396700	7	11	dep	loading	1061:1067	arg1	the					1057:1059	the	1057:1059	the	1057:1059	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	6	12	theme	morphological	790:802	arg1	characterization					804:819	The physico-chemical and morphological characterization	765:819	The physico-chemical and morphological characterization	765:819	The physico-chemical and morphological characterization indicated the successful incorporated of diatomaceous earth within the chitosan membranes.					
29396700	4	13	theme	drug	631:634	arg1	delivery					636:643	dual sublingual drug delivery	615:643	dual sublingual drug delivery	615:643	This work explores the use of the β-chitosan membranes, from marine industry residues, composed with marine sediments for dual sublingual drug delivery.					
29396700	2	14	theme	drug	289:292	arg1	loss					294:297	an important drug loss	276:297	an important drug loss	276:297	For instance, hepatic metabolism means an important drug loss, while some patients have their ability to swell highly compromised (i.e. unconsciousness, cancer…).					
29396700	3	15	theme	Pharmaceutical	436:449	arg1	Form					458:461	an accurate Pharmaceutical Dosage Form	424:461	an accurate Pharmaceutical Dosage Form	424:461	Sublingual placement of an accurate Pharmaceutical Dosage Form is an attractive alternative.					
29396700	0	16	theme	drugs	45:49	arg1	delivery					5:12	Dual delivery	0:12	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.	0:103	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.					
29396700	6	17	theme	physico-chemical	769:784	arg1	characterization					804:819	The physico-chemical and morphological characterization	765:819	The physico-chemical and morphological characterization	765:819	The physico-chemical and morphological characterization indicated the successful incorporated of diatomaceous earth within the chitosan membranes.					
29396700	2	18	theme	important	279:287	arg1	loss					294:297	an important drug loss	276:297	an important drug loss	276:297	For instance, hepatic metabolism means an important drug loss, while some patients have their ability to swell highly compromised (i.e. unconsciousness, cancer…).					
29396700	3	19	theme	Dosage	451:456	arg1	Form					458:461	an accurate Pharmaceutical Dosage Form	424:461	an accurate Pharmaceutical Dosage Form	424:461	Sublingual placement of an accurate Pharmaceutical Dosage Form is an attractive alternative.					
29396700	6	20	theme	chitosan	892:899	arg1	membranes					901:909	the chitosan membranes	888:909	the chitosan membranes	888:909	The physico-chemical and morphological characterization indicated the successful incorporated of diatomaceous earth within the chitosan membranes.					
29396700	5	21	dep	hydrophilic	700:710	arg1	drug					759:762	drug	759:762	drug	759:762	As proof of concept, the membranes were loaded with a hydrophilic (gentamicin) and a hydrophobic (dexamethasone) drug.					
29396700	0	22	theme	Dual	0:3	arg1	delivery					5:12	Dual delivery	0:12	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.	0:103	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.					
29396700	4	23	theme	marine	554:559	arg1	residues					570:577	marine industry residues	554:577	marine industry residues	554:577	This work explores the use of the β-chitosan membranes, from marine industry residues, composed with marine sediments for dual sublingual drug delivery.					
29396700	1	24	theme	Oral	105:108	arg1	administration					110:123	Oral administration	105:123	Oral administration of drugs	105:132	Oral administration of drugs presents important limitations, which are frequently not granted the importance that they really have.					
29396700	3	25	theme	accurate	427:434	arg1	Form					458:461	an accurate Pharmaceutical Dosage Form	424:461	an accurate Pharmaceutical Dosage Form	424:461	Sublingual placement of an accurate Pharmaceutical Dosage Form is an attractive alternative.					
29396700	0	26	theme	hydrophilic	17:27	arg1	drugs					45:49	hydrophilic and hydrophobic drugs	17:49	hydrophilic and hydrophobic drugs	17:49	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.					
29396700	7	27	theme	hydrophobic	1088:1098	arg1	drug					1100:1103	the hydrophobic drug	1084:1103	the hydrophobic drug	1084:1103	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	2	28	dep	swell	342:346	arg1	compromised					355:365	compromised	355:365	to swell highly compromised (i.e. unconsciousness, cancer…)	339:397	For instance, hepatic metabolism means an important drug loss, while some patients have their ability to swell highly compromised (i.e. unconsciousness, cancer…).					
29396700	7	29	theme	drug	1100:1103	arg1	release					1073:1079	release	1073:1079	release	1073:1079	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	7	29	theme	drug	1100:1103	arg1	loading					1061:1067	loading	1061:1067	loading	1061:1067	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	0	30	from	membranes	94:102	arg1	delivery					5:12	Dual delivery	0:12	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.	0:103	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.					
29396700	3	31	theme	attractive	469:478	arg1	alternative					480:490	an attractive alternative	466:490	an attractive alternative	466:490	Sublingual placement of an accurate Pharmaceutical Dosage Form is an attractive alternative.					
29396700	3	31	theme	attractive	469:478	arg1	placement					411:419	Sublingual placement	400:419	Sublingual placement of an accurate Pharmaceutical Dosage Form	400:461	Sublingual placement of an accurate Pharmaceutical Dosage Form is an attractive alternative.					
29396700	0	32	theme	hydrophobic	33:43	arg1	drugs					45:49	hydrophilic and hydrophobic drugs	17:49	hydrophilic and hydrophobic drugs	17:49	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.					
29396700	4	33	theme	industry	561:568	arg1	residues					570:577	marine industry residues	554:577	marine industry residues	554:577	This work explores the use of the β-chitosan membranes, from marine industry residues, composed with marine sediments for dual sublingual drug delivery.					
29396700	3	34	theme	Form	458:461	arg1	alternative					480:490	an attractive alternative	466:490	an attractive alternative	466:490	Sublingual placement of an accurate Pharmaceutical Dosage Form is an attractive alternative.					
29396700	3	34	theme	Form	458:461	arg1	placement					411:419	Sublingual placement	400:419	Sublingual placement of an accurate Pharmaceutical Dosage Form	400:461	Sublingual placement of an accurate Pharmaceutical Dosage Form is an attractive alternative.					
29396700	4	35	theme	β-chitosan	527:536	arg1	membranes					538:546	the β-chitosan membranes	523:546	the β-chitosan membranes	523:546	This work explores the use of the β-chitosan membranes, from marine industry residues, composed with marine sediments for dual sublingual drug delivery.					
29396700	8	36	theme	membranes	1167:1175	arg1	interest					1134:1141	the interest	1130:1141	the interest of the herein developed membranes for dual drug delivery	1130:1198	These results highlight the interest of the herein developed membranes for dual drug delivery.					
29396700	4	37	theme	membranes	538:546	arg1	use					516:518	the use	512:518	the use of the β-chitosan membranes	512:546	This work explores the use of the β-chitosan membranes, from marine industry residues, composed with marine sediments for dual sublingual drug delivery.					
29396700	6	38	theme	diatomaceous	862:873	arg1	earth					875:879	diatomaceous earth	862:879	diatomaceous earth within the chitosan membranes	862:909	The physico-chemical and morphological characterization indicated the successful incorporated of diatomaceous earth within the chitosan membranes.					
29396700	1	39	theme	drugs	128:132	arg1	administration					110:123	Oral administration	105:123	Oral administration of drugs	105:132	Oral administration of drugs presents important limitations, which are frequently not granted the importance that they really have.					
29396700	3	40	theme	Sublingual	400:409	arg1	alternative					480:490	an attractive alternative	466:490	an attractive alternative	466:490	Sublingual placement of an accurate Pharmaceutical Dosage Form is an attractive alternative.					
29396700	3	40	theme	Sublingual	400:409	arg1	placement					411:419	Sublingual placement	400:419	Sublingual placement of an accurate Pharmaceutical Dosage Form	400:461	Sublingual placement of an accurate Pharmaceutical Dosage Form is an attractive alternative.					
29396700	2	41	contain	have	320:323	arg1	patients					311:318	some patients	306:318	some patients	306:318	For instance, hepatic metabolism means an important drug loss, while some patients have their ability to swell highly compromised (i.e. unconsciousness, cancer…).					
29396700	2	41	contain	have	320:323	arg2	ability					331:337	their ability to swell highly compromised (i.e. unconsciousness, cancer…)	325:397	their ability to swell highly compromised (i.e. unconsciousness, cancer…)	325:397	For instance, hepatic metabolism means an important drug loss, while some patients have their ability to swell highly compromised (i.e. unconsciousness, cancer…).					
29396700	7	42	theme	diatomaceous	1029:1040	arg1	earth					1042:1046	diatomaceous earth	1029:1046	diatomaceous earth	1029:1046	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	1	43	theme	important	143:151	arg1	limitations					153:163	important limitations	143:163	important limitations	143:163	Oral administration of drugs presents important limitations, which are frequently not granted the importance that they really have.					
29396700	2	44	dep	unconsciousness	373:387	arg1	i.e.					368:371	i.e.	368:371	i.e.	368:371	For instance, hepatic metabolism means an important drug loss, while some patients have their ability to swell highly compromised (i.e. unconsciousness, cancer…).					
29396700	7	45	theme	drugs	1016:1020	arg1	release					993:999	the immediate release	979:999	the immediate release of hydrophilic drugs	979:1020	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	4	46	dep	explores	503:510	arg1	composed					580:587	composed	580:587	composed with marine sediments for dual sublingual drug delivery	580:643	This work explores the use of the β-chitosan membranes, from marine industry residues, composed with marine sediments for dual sublingual drug delivery.					
29396700	7	47	theme	delivery	917:924	arg1	studies					926:932	Drug delivery studies	912:932	Drug delivery studies	912:932	Drug delivery studies showed the potential of all formulations for the immediate release of hydrophilic drugs, while diatomaceous earth improved the loading and release of the hydrophobic drug.					
29396700	0	48	theme	earth	78:82	arg1	membranes					94:102	chitosan/diatomaceous earth composite membranes	56:102	chitosan/diatomaceous earth composite membranes	56:102	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.					
29396700	4	49	theme	sublingual	620:629	arg1	delivery					636:643	dual sublingual drug delivery	615:643	dual sublingual drug delivery	615:643	This work explores the use of the β-chitosan membranes, from marine industry residues, composed with marine sediments for dual sublingual drug delivery.					
29396700	0	50	theme	chitosan/diatomaceous	56:76	arg1	membranes					94:102	chitosan/diatomaceous earth composite membranes	56:102	chitosan/diatomaceous earth composite membranes	56:102	Dual delivery of hydrophilic and hydrophobic drugs from chitosan/diatomaceous earth composite membranes.					
29396700	2	51	theme	hepatic	251:257	arg1	metabolism					259:268	hepatic metabolism	251:268	hepatic metabolism	251:268	For instance, hepatic metabolism means an important drug loss, while some patients have their ability to swell highly compromised (i.e. unconsciousness, cancer…).					
29396700	4	52	theme	dual	615:618	arg1	delivery					636:643	dual sublingual drug delivery	615:643	dual sublingual drug delivery	615:643	This work explores the use of the β-chitosan membranes, from marine industry residues, composed with marine sediments for dual sublingual drug delivery.					
31783157	11	0	theme	derivative	1714:1723	arg1	transport					1692:1700	passive transport	1684:1700	passive transport of oleyl-HA derivative	1684:1723	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	11	1	theme	carrier	1545:1551	arg1	systems					1553:1559	these carrier systems	1539:1559	these carrier systems	1539:1559	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	5	2	theme	active	853:858	arg1	uptake					869:874	both the passive and active cellular uptake	832:874	uptake	869:874	The regulation of both the passive and active cellular uptake was used to determine the mechanism of micelle internalization.					
31783157	2	3	theme	composition	433:443	arg1	influence					408:416	the influence	404:416	the influence of the carrier composition on the drug penetration	404:467	The aim of this work was to characterize the mechanisms involved in the uptake by skin cells, which is important for understanding the influence of the carrier composition on the drug penetration.					
31783157	0	4	dep	pathway	115:121	arg1	investigation					9:21	In vitro investigation	0:21	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin	0:92	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.					
31783157	11	5	theme	oleyl-HA	1705:1712	arg1	derivative					1714:1723	oleyl-HA derivative	1705:1723	oleyl-HA derivative	1705:1723	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	4	6	theme	micelle	713:719	arg1	payload					721:727	micelle payload	713:727	micelle payload into the keratinocytes and fibroblasts	713:766	This labeling enabled us to track the micelle-forming derivative and also micelle payload into the keratinocytes and fibroblasts by fluorescent microscopy and flow cytometry.					
31783157	1	7	theme	deeper	243:248	arg1	layers					250:255	the deeper layers	239:255	the deeper layers of skin tissue	239:270	In our previous research, we concluded that polymeric micelles based on hyaluronic acid are able to penetrate into the deeper layers of skin tissue.					
31783157	7	8	theme	active	1107:1112	arg1	transport					1126:1134	both active and passive transport	1102:1134	both active and passive transport	1102:1134	Using these methods we concluded that carriers entered the cells using both active and passive transport.					
31783157	5	9	theme	internalization	923:937	arg1	mechanism					902:910	the mechanism	898:910	the mechanism of micelle internalization	898:937	The regulation of both the passive and active cellular uptake was used to determine the mechanism of micelle internalization.					
31783157	11	10	theme	transport	1692:1700	arg1	advantage					1671:1679	the advantage	1667:1679	the advantage of passive transport of oleyl-HA derivative	1667:1723	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	0	11	theme	internalization	99:113	arg1	pathway					115:121	The internalization pathway	95:121	The internalization pathway	95:121	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.					
31783157	2	12	from	influence	408:416	arg1	penetration					457:467	the drug penetration	448:467	the drug penetration	448:467	The aim of this work was to characterize the mechanisms involved in the uptake by skin cells, which is important for understanding the influence of the carrier composition on the drug penetration.					
31783157	8	13	theme	Passive	1137:1143	arg1	transport					1145:1153	Passive transport	1137:1153	Passive transport	1137:1153	Passive transport was facilitated by the affinity of the carrier to the cell membrane, especially in the case of HAC18:1 carrier, which changed significantly the membrane fluidity.					
31783157	5	14	dep	uptake	869:874	arg1	both					832:835	both	832:835	both	832:835	The regulation of both the passive and active cellular uptake was used to determine the mechanism of micelle internalization.					
31783157	8	15	theme	cell	1209:1212	arg1	membrane					1214:1221	the cell membrane	1205:1221	the cell membrane	1205:1221	Passive transport was facilitated by the affinity of the carrier to the cell membrane, especially in the case of HAC18:1 carrier, which changed significantly the membrane fluidity.					
31783157	2	16	theme	skin	355:358	arg1	cells					360:364	skin cells	355:364	skin cells	355:364	The aim of this work was to characterize the mechanisms involved in the uptake by skin cells, which is important for understanding the influence of the carrier composition on the drug penetration.					
31783157	11	17	theme	local	1583:1587	arg1	transport					1589:1597	the local transport	1579:1597	the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative	1579:1723	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	0	18	theme	polymeric	43:51	arg1	micelles					53:60	hyaluronan-based polymeric micelles	26:60	hyaluronan-based polymeric micelles for drug delivery	26:78	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.					
31783157	8	19	theme	membrane	1299:1306	arg1	fluidity					1308:1315	the membrane fluidity	1295:1315	the membrane fluidity	1295:1315	Passive transport was facilitated by the affinity of the carrier to the cell membrane, especially in the case of HAC18:1 carrier, which changed significantly the membrane fluidity.					
31783157	8	20	theme	carrier	1194:1200	arg1	affinity					1178:1185	the affinity	1174:1185	the affinity of the carrier to the cell membrane	1174:1221	Passive transport was facilitated by the affinity of the carrier to the cell membrane, especially in the case of HAC18:1 carrier, which changed significantly the membrane fluidity.					
31783157	11	21	used	used	1570:1573	arg2	systems					1553:1559	these carrier systems	1539:1559	these carrier systems	1539:1559	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	4	22	theme	micelle-forming	677:691	arg1	derivative					693:702	the micelle-forming derivative	673:702	the micelle-forming derivative	673:702	This labeling enabled us to track the micelle-forming derivative and also micelle payload into the keratinocytes and fibroblasts by fluorescent microscopy and flow cytometry.					
31783157	11	23	theme	active	1602:1607	arg1	substances					1609:1618	active substances	1602:1618	active substances	1602:1618	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	1	24	theme	polymeric	168:176	arg1	micelles					178:185	polymeric micelles	168:185	polymeric micelles based on hyaluronic acid	168:210	In our previous research, we concluded that polymeric micelles based on hyaluronic acid are able to penetrate into the deeper layers of skin tissue.					
31783157	0	25	theme	In	0:1	arg1	investigation					9:21	In vitro investigation	0:21	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin	0:92	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.					
31783157	9	26	theme	cell	1356:1359	arg1	type					1361:1364	cell type	1356:1364	cell type	1356:1364	The active transport was dependent on cell type, but mainly driven by the clathrin-mediated endocytosis and macropinocytosis.					
31783157	7	27	theme	passive	1118:1124	arg1	transport					1126:1134	both active and passive transport	1102:1134	both active and passive transport	1102:1134	Using these methods we concluded that carriers entered the cells using both active and passive transport.					
31783157	1	28	theme	skin	260:263	arg1	tissue					265:270	skin tissue	260:270	skin tissue	260:270	In our previous research, we concluded that polymeric micelles based on hyaluronic acid are able to penetrate into the deeper layers of skin tissue.					
31783157	10	29	theme	main	1462:1465	arg1	receptor					1470:1477	the main HA receptor	1458:1477	the main HA receptor	1458:1477	Surprisingly, the main HA receptor, CD44, was not involved in the uptake.					
31783157	10	29	theme	main	1462:1465	arg1	CD44					1480:1483	CD44	1480:1483	CD44	1480:1483	Surprisingly, the main HA receptor, CD44, was not involved in the uptake.					
31783157	11	30	theme	hydrophobic	1623:1633	arg1	drugs					1635:1639	hydrophobic drugs	1623:1639	hydrophobic drugs	1623:1639	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	5	31	theme	micelle	915:921	arg1	internalization					923:937	micelle internalization	915:937	micelle internalization	915:937	The regulation of both the passive and active cellular uptake was used to determine the mechanism of micelle internalization.					
31783157	1	32	theme	tissue	265:270	arg1	layers					250:255	the deeper layers	239:255	the deeper layers of skin tissue	239:270	In our previous research, we concluded that polymeric micelles based on hyaluronic acid are able to penetrate into the deeper layers of skin tissue.					
31783157	11	33	theme	passive	1684:1690	arg1	transport					1692:1700	passive transport	1684:1700	passive transport of oleyl-HA derivative	1684:1723	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	5	34	theme	cellular	860:867	arg1	uptake					869:874	both the passive and active cellular uptake	832:874	uptake	869:874	The regulation of both the passive and active cellular uptake was used to determine the mechanism of micelle internalization.					
31783157	3	35	used	used	493:496	arg2	we					490:491	we	490:491	we	490:491	To reach this goal, we used micelles encapsulating curcumin made of oleyl-hyaluronan (HAC18:1) and hexyl-hyaluronan (HAC6) covalently linked with fluorescent Nile Blue.					
31783157	2	36	theme	carrier	425:431	arg1	composition					433:443	the carrier composition	421:443	the carrier composition	421:443	The aim of this work was to characterize the mechanisms involved in the uptake by skin cells, which is important for understanding the influence of the carrier composition on the drug penetration.					
31783157	11	37	theme	drugs	1635:1639	arg1	transport					1589:1597	the local transport	1579:1597	the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative	1579:1723	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	4	38	theme	fluorescent	771:781	arg1	microscopy					783:792	fluorescent microscopy	771:792	fluorescent microscopy	771:792	This labeling enabled us to track the micelle-forming derivative and also micelle payload into the keratinocytes and fibroblasts by fluorescent microscopy and flow cytometry.					
31783157	9	39	theme	clathrin-mediated	1392:1408	arg1	endocytosis					1410:1420	the clathrin-mediated endocytosis	1388:1420	the clathrin-mediated endocytosis	1388:1420	The active transport was dependent on cell type, but mainly driven by the clathrin-mediated endocytosis and macropinocytosis.					
31783157	1	40	theme	hyaluronic	196:205	arg1	acid					207:210	hyaluronic acid	196:210	hyaluronic acid	196:210	In our previous research, we concluded that polymeric micelles based on hyaluronic acid are able to penetrate into the deeper layers of skin tissue.					
31783157	0	41	theme	hyaluronan-based	26:41	arg1	micelles					53:60	hyaluronan-based polymeric micelles	26:60	hyaluronan-based polymeric micelles for drug delivery	26:78	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.					
31783157	11	42	theme	substances	1609:1618	arg1	transport					1589:1597	the local transport	1579:1597	the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative	1579:1723	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	7	43	dep	concluded	1054:1062	arg1	Using					1031:1035	Using	1031:1035	Using these methods	1031:1049	Using these methods we concluded that carriers entered the cells using both active and passive transport.					
31783157	5	44	theme	passive	841:847	arg1	uptake					869:874	both the passive and active cellular uptake	832:874	uptake	869:874	The regulation of both the passive and active cellular uptake was used to determine the mechanism of micelle internalization.					
31783157	5	45	theme	uptake	869:874	arg1	regulation					818:827	The regulation	814:827	The regulation of both the passive and active cellular uptake	814:874	The regulation of both the passive and active cellular uptake was used to determine the mechanism of micelle internalization.					
31783157	0	46	theme	micelles	53:60	arg1	investigation					9:21	In vitro investigation	0:21	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin	0:92	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.					
31783157	9	47	theme	active	1322:1327	arg1	transport					1329:1337	The active transport	1318:1337	The active transport	1318:1337	The active transport was dependent on cell type, but mainly driven by the clathrin-mediated endocytosis and macropinocytosis.					
31783157	11	48	theme	skin	1650:1653	arg1	cells					1655:1659	the skin cells	1646:1659	the skin cells using the advantage of passive transport of oleyl-HA derivative	1646:1723	We can conclude that these carrier systems could be used for the local transport of active substances or hydrophobic drugs into the skin cells using the advantage of passive transport of oleyl-HA derivative.					
31783157	4	49	theme	flow	798:801	arg1	cytometry					803:811	flow cytometry	798:811	flow cytometry	798:811	This labeling enabled us to track the micelle-forming derivative and also micelle payload into the keratinocytes and fibroblasts by fluorescent microscopy and flow cytometry.					
31783157	8	50	theme	HAC18:1	1250:1256	arg1	carrier					1258:1264	HAC18:1 carrier	1250:1264	HAC18:1 carrier	1250:1264	Passive transport was facilitated by the affinity of the carrier to the cell membrane, especially in the case of HAC18:1 carrier, which changed significantly the membrane fluidity.					
31783157	10	51	theme	HA	1467:1468	arg1	receptor					1470:1477	the main HA receptor	1458:1477	the main HA receptor	1458:1477	Surprisingly, the main HA receptor, CD44, was not involved in the uptake.					
31783157	10	51	theme	HA	1467:1468	arg1	CD44					1480:1483	CD44	1480:1483	CD44	1480:1483	Surprisingly, the main HA receptor, CD44, was not involved in the uptake.					
31783157	5	52	used	used	880:883	arg2	regulation					818:827	The regulation	814:827	The regulation of both the passive and active cellular uptake	814:874	The regulation of both the passive and active cellular uptake was used to determine the mechanism of micelle internalization.					
31783157	2	53	theme	work	289:292	arg1	aim					277:279	The aim	273:279	The aim of this work	273:292	The aim of this work was to characterize the mechanisms involved in the uptake by skin cells, which is important for understanding the influence of the carrier composition on the drug penetration.					
31783157	2	54	theme	drug	452:455	arg1	penetration					457:467	the drug penetration	448:467	the drug penetration	448:467	The aim of this work was to characterize the mechanisms involved in the uptake by skin cells, which is important for understanding the influence of the carrier composition on the drug penetration.					
31783157	1	55	theme	previous	131:138	arg1	research					140:147	our previous research	127:147	our previous research	127:147	In our previous research, we concluded that polymeric micelles based on hyaluronic acid are able to penetrate into the deeper layers of skin tissue.					
31783157	6	56	theme	fluidity	977:984	arg1	changes					957:963	the changes	953:963	the changes of membrane fluidity	953:984	Furthermore, the changes of membrane fluidity were measured for these derivatives by FRAP.					
31783157	0	57	theme	drug	66:69	arg1	delivery					71:78	drug delivery	66:78	drug delivery	66:78	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.					
31783157	0	58	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro investigation of hyaluronan-based polymeric micelles for drug delivery into the skin: The internalization pathway.					
31783157	8	59	theme	carrier	1258:1264	arg1	case					1242:1245	the case	1238:1245	the case of HAC18:1 carrier, which changed significantly the membrane fluidity	1238:1315	Passive transport was facilitated by the affinity of the carrier to the cell membrane, especially in the case of HAC18:1 carrier, which changed significantly the membrane fluidity.					
31783157	6	60	theme	membrane	968:975	arg1	fluidity					977:984	membrane fluidity	968:984	membrane fluidity	968:984	Furthermore, the changes of membrane fluidity were measured for these derivatives by FRAP.					
31426975	2	0	theme	biocompatible	134:146	arg1	copolymers					166:175	New biocompatible amphiphilic block copolymers	130:175	New biocompatible amphiphilic block copolymers	130:175	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	5	1	with	medium	800:805	arg1	50-600 nm					829:837	50-600 nm	829:837	50-600 nm	829:837	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	5	1	with	medium	800:805	arg1	size					823:826	nanometric size	812:826	nanometric size (50-600 nm)	812:838	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	5	1	with	medium	800:805	arg1	form					854:857	spherical form	844:857	spherical form	844:857	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	4	2	theme	length	651:656	arg1	variation					546:554	variation	546:554	variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis	546:737	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	4	3	theme	Different	497:505	arg1	composition					517:527	Different copolymer composition	497:527	Different copolymer composition	497:527	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	2	4	theme	New	130:132	arg1	copolymers					166:175	New biocompatible amphiphilic block copolymers	130:175	New biocompatible amphiphilic block copolymers	130:175	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	6	5	from	Encapsulation	955:967	arg1	micelles					1005:1012	micelles	1005:1012	micelles	1005:1012	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	6	6	theme	times	1036:1040	arg1	solubility					1052:1061	68,181 times its water solubility	1029:1061	68,181 times its water solubility	1029:1061	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	6	7	theme	reduced	1117:1123	arg1	effect					1131:1136	reduced burst effect	1117:1136	reduced burst effect	1117:1136	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	6	8	theme	hydrophobic	976:986	arg1	curcumin					993:1000	the hydrophobic drug curcumin	972:1000	the hydrophobic drug curcumin	972:1000	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	4	9	dep	dextran	580:586	arg1	15 kDa					600:605	15 kDa	600:605	15 kDa	600:605	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	4	9	dep	dextran	580:586	arg1	Mn					589:590	Mn 4.5, 8	589:597	Mn	589:590	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	4	10	theme	ethylenglycol	689:701	arg1	units					703:707	2-4 ethylenglycol units	685:707	2-4 ethylenglycol units	685:707	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	4	10	theme	ethylenglycol	689:701	arg1	oligo					661:665	oligo	661:665	oligo	661:665	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	2	11	theme	deoxycholic	286:296	arg1	acid					280:283	a bile acid	273:283	a bile acid (deoxycholic acid)	273:302	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	2	11	theme	deoxycholic	286:296	arg1	acid					298:301	deoxycholic acid	286:301	deoxycholic acid	286:301	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	3	12	theme	1,3-cycloaddition	348:364	arg1	reaction					366:373	dipolar 1,3-cycloaddition reaction	340:373	dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups	340:494	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	6	13	theme	curcumin	1068:1075	arg1	release					1077:1083	curcumin release	1068:1083	curcumin release from micelles	1068:1097	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	5	14	theme	atomic	893:898	arg1	microscopy					906:915	atomic force microscopy	893:915	atomic force microscopy	893:915	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	3	15	theme	ethylene	442:449	arg1	oligo					436:440	oligo	436:440	oligo	436:440	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	3	15	theme	ethylene	442:449	arg1	glycol					451:456	ethylene glycol	442:456	ethylene glycol	442:456	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	3	16	theme	deoxycholic	417:427	arg1	acid					429:432	deoxycholic acid	417:432	deoxycholic acid	417:432	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	5	17	theme	force	900:904	arg1	microscopy					906:915	atomic force microscopy	893:915	atomic force microscopy	893:915	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	3	18	theme	end	402:404	arg1	groups					406:411	azide end groups	396:411	azide end groups	396:411	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	2	19	theme	block	160:164	arg1	copolymers					166:175	New biocompatible amphiphilic block copolymers	130:175	New biocompatible amphiphilic block copolymers	130:175	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	0	20	theme	Block	0:4	arg1	copolymers					6:15	Block copolymers	0:15	Block copolymers	0:15	Block copolymers containing dextran and deoxycholic acid polyesters.					
31426975	4	21	theme	weights	569:575	arg1	variation					546:554	variation	546:554	variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis	546:737	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	1	22	theme	hydrophobic	98:108	arg1	encapsulation					115:127	hydrophobic drug encapsulation	98:127	hydrophobic drug encapsulation	98:127	Synthesis, self-assembly and hydrophobic drug encapsulation.					
31426975	5	23	theme	nanometric	812:821	arg1	50-600 nm					829:837	50-600 nm	829:837	50-600 nm	829:837	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	5	23	theme	nanometric	812:821	arg1	size					823:826	nanometric size	812:826	nanometric size (50-600 nm)	812:838	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	0	24	theme	dextran	28:34	arg1	polyesters					57:66	dextran and deoxycholic acid polyesters	28:66	dextran and deoxycholic acid polyesters	28:66	Block copolymers containing dextran and deoxycholic acid polyesters.					
31426975	4	25	theme	molecular	559:567	arg1	weights					569:575	molecular weights	559:575	molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa)	559:633	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	1	26	theme	drug	110:113	arg1	encapsulation					115:127	hydrophobic drug encapsulation	98:127	hydrophobic drug encapsulation	98:127	Synthesis, self-assembly and hydrophobic drug encapsulation.					
31426975	4	27	theme	polyester	612:620	arg1	weights					569:575	molecular weights	559:575	molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa)	559:633	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	4	27	theme	polyester	612:620	arg1	length					651:656	the length	647:656	the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis	647:737	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	3	28	theme	azide	396:400	arg1	groups					406:411	azide end groups	396:411	azide end groups	396:411	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	4	29	theme	dextran	580:586	arg1	weights					569:575	molecular weights	559:575	molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa)	559:633	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	4	29	theme	dextran	580:586	arg1	length					651:656	the length	647:656	the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis	647:737	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	5	30	theme	light	875:879	arg1	scattering					881:890	light scattering	875:890	light scattering	875:890	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	6	31	theme	drug	988:991	arg1	curcumin					993:1000	the hydrophobic drug curcumin	972:1000	the hydrophobic drug curcumin	972:1000	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	0	32	theme	deoxycholic	40:50	arg1	acid					52:55	deoxycholic acid	40:55	deoxycholic acid	40:55	Block copolymers containing dextran and deoxycholic acid polyesters.					
31426975	2	33	theme	natural	201:207	arg1	compounds					209:217	two natural compounds	197:217	two natural compounds	197:217	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	2	33	theme	natural	201:207	arg1	materials					231:239	starting materials	222:239	starting materials	222:239	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	6	34	from	micelles	1090:1097	arg1	release					1077:1083	curcumin release	1068:1083	curcumin release from micelles	1068:1097	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	4	35	theme	Mn	623:624	arg1	polyester					612:620	polyester	612:620	polyester (Mn 2-6 kDa)	612:633	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	4	35	theme	Mn	623:624	arg1	2-6 kDa					626:632	Mn 2-6 kDa	623:632	Mn 2-6 kDa	623:632	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	5	36	theme	aqueous	792:798	arg1	medium					800:805	aqueous medium	792:805	aqueous medium with nanometric size (50-600 nm) and spherical form	792:857	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	3	37	with	reaction	366:373	arg1	groups					489:494	propargyl end groups	475:494	propargyl end groups	475:494	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	3	38	theme	s	458:458	arg1	polyester					460:468	s polyester	458:468	s polyester	458:468	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	4	39	theme	oligo	661:665	arg1	weights					569:575	molecular weights	559:575	molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa)	559:633	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	4	39	theme	oligo	661:665	arg1	length					651:656	the length	647:656	the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis	647:737	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	0	40	theme	acid	52:55	arg1	polyesters					57:66	dextran and deoxycholic acid polyesters	28:66	dextran and deoxycholic acid polyesters	28:66	Block copolymers containing dextran and deoxycholic acid polyesters.					
31426975	4	41	theme	copolymer	507:515	arg1	composition					517:527	Different copolymer composition	497:527	Different copolymer composition	497:527	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	3	42	with	dextran	383:389	arg1	groups					406:411	azide end groups	396:411	azide end groups	396:411	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	3	42	with	dextran	383:389	arg1	acid					429:432	deoxycholic acid	417:432	deoxycholic acid	417:432	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	5	43	theme	spherical	844:852	arg1	form					854:857	spherical form	844:857	spherical form	844:857	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	6	44	theme	burst	1125:1129	arg1	effect					1131:1136	reduced burst effect	1117:1136	reduced burst effect	1117:1136	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	4	45	theme	polyester	719:727	arg1	synthesis					729:737	polyester synthesis	719:737	polyester synthesis	719:737	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	2	46	theme	bile	275:278	arg1	acid					280:283	a bile acid	273:283	a bile acid (deoxycholic acid)	273:302	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	2	46	theme	bile	275:278	arg1	acid					298:301	deoxycholic acid	286:301	deoxycholic acid	286:301	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	2	46	theme	bile	275:278	arg1	materials					231:239	starting materials	222:239	starting materials	222:239	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	3	47	theme	propargyl	475:483	arg1	groups					489:494	propargyl end groups	475:494	propargyl end groups	475:494	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	3	48	dep	dextran	383:389	arg1	polyester					460:468	s polyester	458:468	s polyester	458:468	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	2	49	theme	starting	222:229	arg1	acid					280:283	a bile acid	273:283	a bile acid (deoxycholic acid)	273:302	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	2	49	theme	starting	222:229	arg1	compounds					209:217	two natural compounds	197:217	two natural compounds	197:217	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	2	49	theme	starting	222:229	arg1	materials					231:239	starting materials	222:239	starting materials	222:239	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	2	49	theme	starting	222:229	arg1	polysaccharide					244:257	a polysaccharide	242:257	a polysaccharide (dextran)	242:267	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	3	50	theme	end	485:487	arg1	groups					489:494	propargyl end groups	475:494	propargyl end groups	475:494	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	4	51	theme	ethylene	667:674	arg1	oligo					661:665	oligo	661:665	oligo	661:665	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	4	51	theme	ethylene	667:674	arg1	glycol					676:681	ethylene glycol	667:681	ethylene glycol	667:681	Different copolymer composition were obtained by variation of molecular weights of dextran (Mn 4.5, 8, 15 kDa) and polyester (Mn 2-6 kDa), as well as the length of oligo(ethylene glycol) (2-4 ethylenglycol units) used for polyester synthesis.					
31426975	5	52	theme	transmission	921:932	arg1	microscopy					943:952	transmission electron microscopy	921:952	transmission electron microscopy	921:952	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
31426975	6	53	theme	curcumin	993:1000	arg1	Encapsulation					955:967	Encapsulation	955:967	Encapsulation of the hydrophobic drug curcumin in micelles	955:1012	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	6	54	theme	water	1046:1050	arg1	solubility					1052:1061	68,181 times its water solubility	1029:1061	68,181 times its water solubility	1029:1061	Encapsulation of the hydrophobic drug curcumin in micelles could increase 68,181 times its water solubility, and curcumin release from micelles was slow and with reduced burst effect.					
31426975	3	55	theme	dipolar	340:346	arg1	reaction					366:373	dipolar 1,3-cycloaddition reaction	340:373	dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups	340:494	The copolymers were synthesized by dipolar 1,3-cycloaddition reaction between dextran with azide end groups and deoxycholic acid - oligo(ethylene glycol)s polyester with propargyl end groups.					
31426975	2	56	theme	amphiphilic	148:158	arg1	copolymers					166:175	New biocompatible amphiphilic block copolymers	130:175	New biocompatible amphiphilic block copolymers	130:175	New biocompatible amphiphilic block copolymers were prepared using two natural compounds as starting materials, a polysaccharide (dextran) and a bile acid (deoxycholic acid).					
31426975	5	57	theme	electron	934:941	arg1	microscopy					943:952	transmission electron microscopy	921:952	transmission electron microscopy	921:952	These copolymers can for micelle like aggregates in aqueous medium with nanometric size (50-600 nm) and spherical form, as assessed by light scattering, atomic force microscopy and transmission electron microscopy.					
30336512	4	0	from	phase	605:609	arg1	low					586:588	low	586:588	low	586:588	Droplet size was still low in the gastric phase and pseudoplasticity was well preserved (even though viscosity decreased) during in vitro gastrointestinal digestion.					
30336512	5	1	from	reduction	815:823	arg1	level					845:849	the triglyceride level	828:849	the triglyceride level in the blood of rats, fed by the bilayer emulsion	828:899	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	4	2	theme	gastrointestinal	701:716	arg1	digestion					718:726	in vitro gastrointestinal digestion	692:726	in vitro gastrointestinal digestion	692:726	Droplet size was still low in the gastric phase and pseudoplasticity was well preserved (even though viscosity decreased) during in vitro gastrointestinal digestion.					
30336512	0	3	theme	In Vivo	75:81	arg1	Models					83:88	In Vitro and In Vivo Models	62:88	In Vitro and In Vivo Models	62:88	Impact of Interfacial Composition on Emulsion Digestion Using In Vitro and In Vivo Models.					
30336512	5	4	from	emulsions	800:808	arg1	bioavailability					771:785	a lower fat bioavailability	759:785	a lower fat bioavailability from bilayer emulsions	759:808	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	4	5	theme	in vitro	692:699	arg1	digestion					718:726	in vitro gastrointestinal digestion	692:726	in vitro gastrointestinal digestion	692:726	Droplet size was still low in the gastric phase and pseudoplasticity was well preserved (even though viscosity decreased) during in vitro gastrointestinal digestion.					
30336512	0	6	from	Impact	0:5	arg1	Digestion					46:54	Emulsion Digestion	37:54	Emulsion Digestion Using In Vitro and In Vivo Models	37:88	Impact of Interfacial Composition on Emulsion Digestion Using In Vitro and In Vivo Models.					
30336512	5	7	theme	bilayer	884:890	arg1	emulsion					892:899	the bilayer emulsion	880:899	the bilayer emulsion	880:899	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	9	8	theme	low	1358:1360	arg1	bioavailability					1366:1380	low fat bioavailability	1358:1380	low fat bioavailability	1358:1380	Such behavior of the bilayer emulsion made it interesting for the formulation of food products with low fat bioavailability.					
30336512	2	9	theme	oil	285:287	arg1	emulsion					264:271	The monolayer emulsion	250:271	The monolayer emulsion of rapeseed oil	250:287	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	1	10	theme	different	128:136	arg1	layers					153:158	different emulsification layers	128:158	different emulsification layers as mono- and bilayers	128:180	This study explored the influence of different emulsification layers as mono- and bilayers on lipid digestion by using in vitro and in vivo digestion methods.					
30336512	4	11	from	low	586:588	arg1	phase					605:609	the gastric phase	593:609	the gastric phase	593:609	Droplet size was still low in the gastric phase and pseudoplasticity was well preserved (even though viscosity decreased) during in vitro gastrointestinal digestion.					
30336512	5	12	from	level	845:849	arg1	blood					858:862	the blood	854:862	the blood of rats, fed by the bilayer emulsion	854:899	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	3	13	theme	fatty	482:486	arg1	acids					488:492	free fatty acids	477:492	free fatty acids	477:492	The in vitro digestion using human gastrointestinal enzymes showed that the lipid digestion as free fatty acids was slowed down in the bilayer emulsion compared with the monolayer.					
30336512	2	14	theme	rapeseed	276:283	arg1	oil					285:287	rapeseed oil	276:287	rapeseed oil	276:287	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	5	15	theme	rats	867:870	arg1	blood					858:862	the blood	854:862	the blood of rats, fed by the bilayer emulsion	854:899	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	5	16	theme	lower	761:765	arg1	bioavailability					771:785	a lower fat bioavailability	759:785	a lower fat bioavailability from bilayer emulsions	759:808	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	9	17	theme	products	1344:1351	arg1	formulation					1324:1334	the formulation	1320:1334	the formulation of food products with low fat bioavailability	1320:1380	Such behavior of the bilayer emulsion made it interesting for the formulation of food products with low fat bioavailability.					
30336512	3	18	theme	gastrointestinal	417:432	arg1	enzymes					434:440	human gastrointestinal enzymes	411:440	human gastrointestinal enzymes	411:440	The in vitro digestion using human gastrointestinal enzymes showed that the lipid digestion as free fatty acids was slowed down in the bilayer emulsion compared with the monolayer.					
30336512	4	19	theme	gastric	597:603	arg1	phase					605:609	the gastric phase	593:609	the gastric phase	593:609	Droplet size was still low in the gastric phase and pseudoplasticity was well preserved (even though viscosity decreased) during in vitro gastrointestinal digestion.					
30336512	3	20	theme	lipid	458:462	arg1	digestion					464:472	the lipid digestion	454:472	the lipid digestion as free fatty acids	454:492	The in vitro digestion using human gastrointestinal enzymes showed that the lipid digestion as free fatty acids was slowed down in the bilayer emulsion compared with the monolayer.					
30336512	3	21	theme	in vitro	386:393	arg1	digestion					395:403	The in vitro digestion	382:403	The in vitro digestion using human gastrointestinal enzymes	382:440	The in vitro digestion using human gastrointestinal enzymes showed that the lipid digestion as free fatty acids was slowed down in the bilayer emulsion compared with the monolayer.					
30336512	5	22	theme	bilayer	792:798	arg1	emulsions					800:808	bilayer emulsions	792:808	bilayer emulsions	792:808	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	6	23	theme	bilayer	968:974	arg1	emulsion					976:983	the bilayer emulsion	964:983	the bilayer emulsion	964:983	The results clearly showed that lipid digestion was slower in the bilayer emulsion than in the monolayer.					
30336512	9	24	theme	food	1339:1342	arg1	products					1344:1351	food products	1339:1351	food products with low fat bioavailability	1339:1380	Such behavior of the bilayer emulsion made it interesting for the formulation of food products with low fat bioavailability.					
30336512	9	25	theme	fat	1362:1364	arg1	bioavailability					1366:1380	low fat bioavailability	1358:1380	low fat bioavailability	1358:1380	Such behavior of the bilayer emulsion made it interesting for the formulation of food products with low fat bioavailability.					
30336512	4	26	theme	Droplet	563:569	arg1	size					571:574	Droplet size	563:574	Droplet size	563:574	Droplet size was still low in the gastric phase and pseudoplasticity was well preserved (even though viscosity decreased) during in vitro gastrointestinal digestion.					
30336512	1	27	theme	lipid	185:189	arg1	digestion					191:199	lipid digestion	185:199	lipid digestion	185:199	This study explored the influence of different emulsification layers as mono- and bilayers on lipid digestion by using in vitro and in vivo digestion methods.					
30336512	0	28	theme	Composition	22:32	arg1	Impact					0:5	Impact	0:5	Impact of Interfacial Composition on Emulsion Digestion Using In Vitro and In Vivo Models.	0:89	Impact of Interfacial Composition on Emulsion Digestion Using In Vitro and In Vivo Models.					
30336512	8	29	dep	APPLICATION	1113:1123	arg1	allows					1189:1194	allows	1189:1194	allows the preparation of emulsions with slower fat bioavailability	1189:1255	PRACTICAL APPLICATION: The layer-by-layer production approach that was presented here allows the preparation of emulsions with slower fat bioavailability.					
30336512	1	30	from	influence	115:123	arg1	digestion					191:199	lipid digestion	185:199	lipid digestion	185:199	This study explored the influence of different emulsification layers as mono- and bilayers on lipid digestion by using in vitro and in vivo digestion methods.					
30336512	8	31	theme	fat	1237:1239	arg1	bioavailability					1241:1255	slower fat bioavailability	1230:1255	slower fat bioavailability	1230:1255	PRACTICAL APPLICATION: The layer-by-layer production approach that was presented here allows the preparation of emulsions with slower fat bioavailability.					
30336512	3	32	theme	free	477:480	arg1	acids					488:492	free fatty acids	477:492	free fatty acids	477:492	The in vitro digestion using human gastrointestinal enzymes showed that the lipid digestion as free fatty acids was slowed down in the bilayer emulsion compared with the monolayer.					
30336512	0	33	theme	Interfacial	10:20	arg1	Composition					22:32	Interfacial Composition	10:32	Interfacial Composition	10:32	Impact of Interfacial Composition on Emulsion Digestion Using In Vitro and In Vivo Models.					
30336512	7	34	theme	emulsions	1077:1085	arg1	behavior					1065:1072	the behavior	1061:1072	the behavior of emulsions upon digestion	1061:1100	These results provide bio-relevant information about the behavior of emulsions upon digestion.					
30336512	9	35	theme	Such	1258:1261	arg1	behavior					1263:1270	Such behavior	1258:1270	Such behavior of the bilayer emulsion	1258:1294	Such behavior of the bilayer emulsion made it interesting for the formulation of food products with low fat bioavailability.					
30336512	2	36	theme	whey	299:302	arg1	proteins					304:311	whey proteins	299:311	whey proteins	299:311	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	3	37	theme	bilayer	517:523	arg1	emulsion					525:532	the bilayer emulsion	513:532	the bilayer emulsion	513:532	The in vitro digestion using human gastrointestinal enzymes showed that the lipid digestion as free fatty acids was slowed down in the bilayer emulsion compared with the monolayer.					
30336512	0	38	theme	Emulsion	37:44	arg1	Digestion					46:54	Emulsion Digestion	37:54	Emulsion Digestion Using In Vitro and In Vivo Models	37:88	Impact of Interfacial Composition on Emulsion Digestion Using In Vitro and In Vivo Models.					
30336512	2	39	contain	contained	289:297	arg2	cellulose					371:379	carboxymethyl cellulose	357:379	carboxymethyl cellulose	357:379	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	2	39	contain	contained	289:297	arg2	proteins					304:311	whey proteins	299:311	whey proteins	299:311	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	2	39	contain	contained	289:297	arg2	proteins					344:351	whey proteins	339:351	whey proteins	339:351	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	2	39	contain	contained	289:297	arg2	emulsion					329:336	the bilayer emulsion	317:336	the bilayer emulsion	317:336	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	2	39	contain	contained	289:297	arg1	emulsion					264:271	The monolayer emulsion	250:271	The monolayer emulsion of rapeseed oil	250:287	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	8	40	theme	PRACTICAL	1103:1111	arg1	APPLICATION					1113:1123	PRACTICAL APPLICATION	1103:1123	PRACTICAL APPLICATION: The layer-by-layer production approach that was presented here allows the preparation of emulsions with slower fat bioavailability.	1103:1256	PRACTICAL APPLICATION: The layer-by-layer production approach that was presented here allows the preparation of emulsions with slower fat bioavailability.					
30336512	8	41	theme	slower	1230:1235	arg1	bioavailability					1241:1255	slower fat bioavailability	1230:1255	slower fat bioavailability	1230:1255	PRACTICAL APPLICATION: The layer-by-layer production approach that was presented here allows the preparation of emulsions with slower fat bioavailability.					
30336512	5	42	theme	in vivo	733:739	arg1	studies					741:747	The in vivo studies	729:747	The in vivo studies	729:747	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	4	43	dep	preserved	641:649	arg1	even					652:655	even	652:655	even	652:655	Droplet size was still low in the gastric phase and pseudoplasticity was well preserved (even though viscosity decreased) during in vitro gastrointestinal digestion.					
30336512	1	44	theme	in vitro	210:217	arg1	methods					241:247	in vitro and in vivo digestion methods	210:247	in vitro and in vivo digestion methods	210:247	This study explored the influence of different emulsification layers as mono- and bilayers on lipid digestion by using in vitro and in vivo digestion methods.					
30336512	8	45	theme	production	1145:1154	arg1	approach					1156:1163	The layer-by-layer production approach	1126:1163	The layer-by-layer production approach that was presented here	1126:1187	PRACTICAL APPLICATION: The layer-by-layer production approach that was presented here allows the preparation of emulsions with slower fat bioavailability.					
30336512	5	46	theme	triglyceride	832:843	arg1	level					845:849	the triglyceride level	828:849	the triglyceride level in the blood of rats, fed by the bilayer emulsion	828:899	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	2	47	theme	whey	339:342	arg1	proteins					344:351	whey proteins	339:351	whey proteins	339:351	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	3	48	theme	human	411:415	arg1	enzymes					434:440	human gastrointestinal enzymes	411:440	human gastrointestinal enzymes	411:440	The in vitro digestion using human gastrointestinal enzymes showed that the lipid digestion as free fatty acids was slowed down in the bilayer emulsion compared with the monolayer.					
30336512	6	49	theme	lipid	934:938	arg1	digestion					940:948	lipid digestion	934:948	lipid digestion	934:948	The results clearly showed that lipid digestion was slower in the bilayer emulsion than in the monolayer.					
30336512	9	50	theme	bilayer	1279:1285	arg1	emulsion					1287:1294	the bilayer emulsion	1275:1294	the bilayer emulsion	1275:1294	Such behavior of the bilayer emulsion made it interesting for the formulation of food products with low fat bioavailability.					
30336512	1	51	theme	layers	153:158	arg1	influence					115:123	the influence	111:123	the influence of different emulsification layers as mono- and bilayers on lipid digestion	111:199	This study explored the influence of different emulsification layers as mono- and bilayers on lipid digestion by using in vitro and in vivo digestion methods.					
30336512	9	52	with	products	1344:1351	arg1	bioavailability					1366:1380	low fat bioavailability	1358:1380	low fat bioavailability	1358:1380	Such behavior of the bilayer emulsion made it interesting for the formulation of food products with low fat bioavailability.					
30336512	8	53	theme	layer-by-layer	1130:1143	arg1	approach					1156:1163	The layer-by-layer production approach	1126:1163	The layer-by-layer production approach that was presented here	1126:1187	PRACTICAL APPLICATION: The layer-by-layer production approach that was presented here allows the preparation of emulsions with slower fat bioavailability.					
30336512	9	54	theme	emulsion	1287:1294	arg1	behavior					1263:1270	Such behavior	1258:1270	Such behavior of the bilayer emulsion	1258:1294	Such behavior of the bilayer emulsion made it interesting for the formulation of food products with low fat bioavailability.					
30336512	7	55	theme	bio-relevant	1030:1041	arg1	information					1043:1053	bio-relevant information	1030:1053	bio-relevant information about the behavior of emulsions upon digestion	1030:1100	These results provide bio-relevant information about the behavior of emulsions upon digestion.					
30336512	2	56	theme	monolayer	254:262	arg1	emulsion					264:271	The monolayer emulsion	250:271	The monolayer emulsion of rapeseed oil	250:287	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	2	57	theme	bilayer	321:327	arg1	emulsion					329:336	the bilayer emulsion	317:336	the bilayer emulsion	317:336	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
30336512	1	58	theme	in vivo	223:229	arg1	digestion					231:239	in vivo digestion	223:239	in vivo digestion	223:239	This study explored the influence of different emulsification layers as mono- and bilayers on lipid digestion by using in vitro and in vivo digestion methods.					
30336512	5	59	theme	fat	767:769	arg1	bioavailability					771:785	a lower fat bioavailability	759:785	a lower fat bioavailability from bilayer emulsions	759:808	The in vivo studies confirmed a lower fat bioavailability from bilayer emulsions by a reduction in the triglyceride level in the blood of rats, fed by the bilayer emulsion.					
30336512	1	60	theme	emulsification	138:151	arg1	layers					153:158	different emulsification layers	128:158	different emulsification layers as mono- and bilayers	128:180	This study explored the influence of different emulsification layers as mono- and bilayers on lipid digestion by using in vitro and in vivo digestion methods.					
30336512	1	61	theme	digestion	231:239	arg1	methods					241:247	in vitro and in vivo digestion methods	210:247	in vitro and in vivo digestion methods	210:247	This study explored the influence of different emulsification layers as mono- and bilayers on lipid digestion by using in vitro and in vivo digestion methods.					
30336512	0	62	theme	In Vitro	62:69	arg1	Models					83:88	In Vitro and In Vivo Models	62:88	In Vitro and In Vivo Models	62:88	Impact of Interfacial Composition on Emulsion Digestion Using In Vitro and In Vivo Models.					
30336512	8	63	theme	emulsions	1215:1223	arg1	preparation					1200:1210	the preparation	1196:1210	the preparation of emulsions	1196:1223	PRACTICAL APPLICATION: The layer-by-layer production approach that was presented here allows the preparation of emulsions with slower fat bioavailability.					
30336512	2	64	theme	carboxymethyl	357:369	arg1	cellulose					371:379	carboxymethyl cellulose	357:379	carboxymethyl cellulose	357:379	The monolayer emulsion of rapeseed oil contained whey proteins and the bilayer emulsion, whey proteins and carboxymethyl cellulose.					
29421058	5	0	theme	monosaccharide	677:690	arg1	composition					692:702	the monosaccharide composition	673:702	the monosaccharide composition	673:702	The backbone structure was confirmed by the monosaccharide composition and two-dimensional NMR.					
29421058	6	1	from	abilities	793:801	arg1	1,1-diphenyl-2-picrylhydrazyl					806:834	1,1-diphenyl-2-picrylhydrazyl	806:834	1,1-diphenyl-2-picrylhydrazyl	806:834	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	6	1	from	abilities	793:801	arg1	inhibition					863:872	inhibition	863:872	inhibition of lipid peroxidation	863:894	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	6	1	from	abilities	793:801	arg1	radicals					850:857	nitric oxide radicals	837:857	nitric oxide radicals	837:857	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	7	2	theme	sulfation	963:971	arg1	patterns					973:980	the sulfation patterns	959:980	the sulfation patterns of the fucose branches	959:1003	The results showed that their activities could be affected by the sulfation patterns of the fucose branches, and O-4 sulfation is particularly important for its activities.					
29421058	0	3	theme	sulfate	95:101	arg1	properties					57:66	antioxidant and anti-inflammatory properties	23:66	antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers	23:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	0	3	theme	sulfate	95:101	arg1	comparison					11:20	Structural comparison	0:20	Structural comparison	0:20	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	4	4	theme	branches	547:554	arg1	patterns					528:535	three sulfation patterns	512:535	three sulfation patterns of fucose branches	512:554	1H and 13C NMR of the polysaccharide identified three sulfation patterns of fucose branches: 4-O-, 2,4-di-O, and 3,4-di-O-sulfation variously existed in different fCSs.					
29421058	8	5	theme	fCS-Am	1102:1107	arg1	assays					1092:1097	The anti-inflammatory assays	1070:1097	The anti-inflammatory assays of fCS-Am	1070:1107	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	8	6	theme	significant	1116:1126	arg1	reduction					1128:1136	significant reduction	1116:1136	significant reduction of the carrageenan induced edema	1116:1169	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	8	7	used	used	1213:1216	arg2	agent					1246:1250	a potential antiallergic agent	1221:1250	a potential antiallergic agent	1221:1250	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	8	7	used	used	1213:1216	arg2	manner					1190:1195	a dose depended manner	1174:1195	a dose depended manner	1174:1195	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	0	8	theme	edible	112:117	arg1	cucumbers					123:131	three edible sea cucumbers	106:131	three edible sea cucumbers	106:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	4	9	theme	fucose	540:545	arg1	branches					547:554	fucose branches	540:554	fucose branches	540:554	1H and 13C NMR of the polysaccharide identified three sulfation patterns of fucose branches: 4-O-, 2,4-di-O, and 3,4-di-O-sulfation variously existed in different fCSs.					
29421058	8	10	dep	potential	1223:1231	arg1	antiallergic					1233:1244	antiallergic	1233:1244	antiallergic	1233:1244	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	6	11	theme	lipid	877:881	arg1	peroxidation					883:894	lipid peroxidation	877:894	lipid peroxidation	877:894	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	0	12	gly	fucosylated	71:81	arg1	sulfate					95:101	fucosylated chondroitin sulfate	71:101	fucosylated chondroitin sulfate of three edible sea cucumbers	71:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	5	13	theme	two-dimensional	708:722	arg1	NMR					724:726	two-dimensional NMR	708:726	two-dimensional NMR	708:726	The backbone structure was confirmed by the monosaccharide composition and two-dimensional NMR.					
29421058	5	14	theme	backbone	637:644	arg1	structure					646:654	The backbone structure	633:654	The backbone structure	633:654	The backbone structure was confirmed by the monosaccharide composition and two-dimensional NMR.					
29421058	8	15	dep	induced	1157:1163	arg1	carrageenan					1145:1155	carrageenan	1145:1155	carrageenan	1145:1155	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	4	16	theme	different	617:625	arg1	fCSs					627:630	different fCSs	617:630	different fCSs	617:630	1H and 13C NMR of the polysaccharide identified three sulfation patterns of fucose branches: 4-O-, 2,4-di-O, and 3,4-di-O-sulfation variously existed in different fCSs.					
29421058	0	17	theme	cucumbers	123:131	arg1	sulfate					95:101	fucosylated chondroitin sulfate	71:101	fucosylated chondroitin sulfate of three edible sea cucumbers	71:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	4	18	theme	polysaccharide	486:499	arg1	1H					464:465	1H	464:465	1H	464:465	1H and 13C NMR of the polysaccharide identified three sulfation patterns of fucose branches: 4-O-, 2,4-di-O, and 3,4-di-O-sulfation variously existed in different fCSs.					
29421058	4	18	theme	polysaccharide	486:499	arg1	13C					471:473	13C	471:473	13C	471:473	1H and 13C NMR of the polysaccharide identified three sulfation patterns of fucose branches: 4-O-, 2,4-di-O, and 3,4-di-O-sulfation variously existed in different fCSs.					
29421058	0	19	theme	sea	119:121	arg1	cucumbers					123:131	three edible sea cucumbers	106:131	three edible sea cucumbers	106:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	4	20	theme	sulfation	518:526	arg1	patterns					528:535	three sulfation patterns	512:535	three sulfation patterns of fucose branches	512:554	1H and 13C NMR of the polysaccharide identified three sulfation patterns of fucose branches: 4-O-, 2,4-di-O, and 3,4-di-O-sulfation variously existed in different fCSs.					
29421058	8	21	dep	dose	1176:1179	arg1	depended					1181:1188	depended	1181:1188	depended	1181:1188	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	8	22	theme	anti-inflammatory	1074:1090	arg1	assays					1092:1097	The anti-inflammatory assays	1070:1097	The anti-inflammatory assays of fCS-Am	1070:1107	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	6	23	theme	fCSs	755:758	arg1	properties					741:750	Antioxidant properties	729:750	Antioxidant properties of fCSs	729:758	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	3	24	theme	fucose	415:420	arg1	branches					422:429	particularly the fucose branches	398:429	particularly the fucose branches	398:429	The detailed structures of fCSs, particularly the fucose branches, were investigated and compared.					
29421058	3	24	theme	fucose	415:420	arg1	structures					378:387	The detailed structures	365:387	The detailed structures of fCSs	365:395	The detailed structures of fCSs, particularly the fucose branches, were investigated and compared.					
29421058	8	25	theme	induced	1157:1163	arg1	edema					1165:1169	the carrageenan induced edema	1141:1169	the carrageenan induced edema	1141:1169	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	7	26	theme	O-4	1010:1012	arg1	sulfation					1014:1022	O-4 sulfation	1010:1022	O-4 sulfation	1010:1022	The results showed that their activities could be affected by the sulfation patterns of the fucose branches, and O-4 sulfation is particularly important for its activities.					
29421058	0	27	theme	Structural	0:9	arg1	properties					57:66	antioxidant and anti-inflammatory properties	23:66	antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers	23:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	0	27	theme	Structural	0:9	arg1	comparison					11:20	Structural comparison	0:20	Structural comparison	0:20	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	7	28	theme	branches	996:1003	arg1	patterns					973:980	the sulfation patterns	959:980	the sulfation patterns of the fucose branches	959:1003	The results showed that their activities could be affected by the sulfation patterns of the fucose branches, and O-4 sulfation is particularly important for its activities.					
29421058	1	29	theme	edible	197:202	arg1	cucumbers					208:216	edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea	197:288	edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China	197:309	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	0	30	theme	antioxidant	23:33	arg1	properties					57:66	antioxidant and anti-inflammatory properties	23:66	antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers	23:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	0	30	theme	antioxidant	23:33	arg1	comparison					11:20	Structural comparison	0:20	Structural comparison	0:20	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	6	31	theme	oxide	844:848	arg1	radicals					850:857	nitric oxide radicals	837:857	nitric oxide radicals	837:857	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	6	32	theme	Antioxidant	729:739	arg1	properties					741:750	Antioxidant properties	729:750	Antioxidant properties of fCSs	729:758	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	1	33	theme	sea	204:206	arg1	cucumbers					208:216	edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea	197:288	edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China	197:309	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	8	34	theme	edema	1165:1169	arg1	reduction					1128:1136	significant reduction	1116:1136	significant reduction of the carrageenan induced edema	1116:1169	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	2	35	theme	Stichopus	321:329	arg1	chloronotus					331:341	Stichopus chloronotus	321:341	Stichopus chloronotus	321:341	fCS from Stichopus chloronotus was firstly reported.					
29421058	0	36	theme	anti-inflammatory	39:55	arg1	properties					57:66	antioxidant and anti-inflammatory properties	23:66	antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers	23:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	0	36	theme	anti-inflammatory	39:55	arg1	comparison					11:20	Structural comparison	0:20	Structural comparison	0:20	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	8	37	theme	dose	1176:1179	arg1	manner					1190:1195	a dose depended manner	1174:1195	a dose depended manner	1174:1195	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	8	37	theme	dose	1176:1179	arg1	agent					1246:1250	a potential antiallergic agent	1221:1250	a potential antiallergic agent	1221:1250	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	1	38	gly	fucosylated	140:150	arg1	fCS					173:175	fCS	173:175	fCS	173:175	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	1	38	gly	fucosylated	140:150	arg1	sulfate					164:170	Three fucosylated chondroitin sulfate	134:170	Three fucosylated chondroitin sulfate (fCS)	134:176	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	6	39	theme	nitric	837:842	arg1	oxide					844:848	nitric oxide	837:848	nitric oxide radicals	837:857	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	3	40	theme	fCSs	392:395	arg1	branches					422:429	particularly the fucose branches	398:429	particularly the fucose branches	398:429	The detailed structures of fCSs, particularly the fucose branches, were investigated and compared.					
29421058	3	40	theme	fCSs	392:395	arg1	structures					378:387	The detailed structures	365:387	The detailed structures of fCSs	365:395	The detailed structures of fCSs, particularly the fucose branches, were investigated and compared.					
29421058	3	41	theme	detailed	369:376	arg1	branches					422:429	particularly the fucose branches	398:429	particularly the fucose branches	398:429	The detailed structures of fCSs, particularly the fucose branches, were investigated and compared.					
29421058	3	41	theme	detailed	369:376	arg1	structures					378:387	The detailed structures	365:387	The detailed structures of fCSs	365:395	The detailed structures of fCSs, particularly the fucose branches, were investigated and compared.					
29421058	6	42	theme	peroxidation	883:894	arg1	1,1-diphenyl-2-picrylhydrazyl					806:834	1,1-diphenyl-2-picrylhydrazyl	806:834	1,1-diphenyl-2-picrylhydrazyl	806:834	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	6	42	theme	peroxidation	883:894	arg1	inhibition					863:872	inhibition	863:872	inhibition of lipid peroxidation	863:894	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	6	42	theme	peroxidation	883:894	arg1	radicals					850:857	nitric oxide radicals	837:857	nitric oxide radicals	837:857	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	1	43	theme	fucosylated	140:150	arg1	fCS					173:175	fCS	173:175	fCS	173:175	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	1	43	theme	fucosylated	140:150	arg1	sulfate					164:170	Three fucosylated chondroitin sulfate	134:170	Three fucosylated chondroitin sulfate (fCS)	134:176	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	7	44	theme	fucose	989:994	arg1	branches					996:1003	the fucose branches	985:1003	the fucose branches	985:1003	The results showed that their activities could be affected by the sulfation patterns of the fucose branches, and O-4 sulfation is particularly important for its activities.					
29421058	8	45	theme	potential	1223:1231	arg1	manner					1190:1195	a dose depended manner	1174:1195	a dose depended manner	1174:1195	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	8	45	theme	potential	1223:1231	arg1	agent					1246:1250	a potential antiallergic agent	1221:1250	a potential antiallergic agent	1221:1250	The anti-inflammatory assays of fCS-Am showed significant reduction of the carrageenan induced edema in a dose depended manner, which could be used as a potential antiallergic agent.					
29421058	1	46	theme	chondroitin	152:162	arg1	fCS					173:175	fCS	173:175	fCS	173:175	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	1	46	theme	chondroitin	152:162	arg1	sulfate					164:170	Three fucosylated chondroitin sulfate	134:170	Three fucosylated chondroitin sulfate (fCS)	134:176	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	4	47	dep	1H	464:465	arg1	NMR					475:477	NMR	475:477	NMR	475:477	1H and 13C NMR of the polysaccharide identified three sulfation patterns of fucose branches: 4-O-, 2,4-di-O, and 3,4-di-O-sulfation variously existed in different fCSs.					
29421058	0	48	theme	chondroitin	83:93	arg1	sulfate					95:101	fucosylated chondroitin sulfate	71:101	fucosylated chondroitin sulfate of three edible sea cucumbers	71:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	6	49	theme	scavenging	782:791	arg1	abilities					793:801	the scavenging abilities	778:801	the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation	778:894	Antioxidant properties of fCSs were evaluated by the scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl, nitric oxide radicals and inhibition of lipid peroxidation.					
29421058	0	50	theme	fucosylated	71:81	arg1	sulfate					95:101	fucosylated chondroitin sulfate	71:101	fucosylated chondroitin sulfate of three edible sea cucumbers	71:131	Structural comparison, antioxidant and anti-inflammatory properties of fucosylated chondroitin sulfate of three edible sea cucumbers.					
29421058	1	51	dep	cucumbers	208:216	arg1	chloronotus					252:262	Stichopus chloronotus	242:262	Stichopus chloronotus	242:262	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	1	51	dep	cucumbers	208:216	arg1	molpadioidea					277:288	Acaudina molpadioidea	268:288	Acaudina molpadioidea	268:288	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	1	51	dep	cucumbers	208:216	arg1	japonicus					231:239	Apostichopus japonicus	218:239	Apostichopus japonicus	218:239	Three fucosylated chondroitin sulfate (fCS) were obtained from edible sea cucumbers Apostichopus japonicus, Stichopus chloronotus and Acaudina molpadioidea collected from China.					
29421058	2	52	from	chloronotus	331:341	arg1	fCS					312:314	fCS	312:314	fCS from Stichopus chloronotus	312:341	fCS from Stichopus chloronotus was firstly reported.					
29611461	5	0	contain	had	674:676	arg2	properties					701:710	excellent slow-release properties	678:710	excellent slow-release properties	678:710	The tribology results showed that the PEG/CS/GP gel had excellent slow-release properties, especially under pressure, and the PEG played an important role in improving the gel's rheological and mechanical properties.					
29611461	5	0	contain	had	674:676	arg1	gel					670:672	the PEG/CS/GP gel	656:672	the PEG/CS/GP gel	656:672	The tribology results showed that the PEG/CS/GP gel had excellent slow-release properties, especially under pressure, and the PEG played an important role in improving the gel's rheological and mechanical properties.					
29611461	0	1	theme	joint	72:76	arg1	capsule					78:84	the joint capsule	68:84	the joint capsule with slow-release lubricant after joint surgery	68:132	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel replaced the joint capsule with slow-release lubricant after joint surgery.					
29611461	0	2	with	capsule	78:84	arg1	lubricant					104:112	lubricant	104:112	lubricant	104:112	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel replaced the joint capsule with slow-release lubricant after joint surgery.					
29611461	2	3	theme	body	342:345	arg1	fluids					347:352	body fluids	342:352	body fluids	342:352	However, traditional lubricant would be diluted by body fluids and then absorbed by the human body.					
29611461	1	4	theme	body	250:253	arg1	fluid					255:259	body fluid	250:259	body fluid	250:259	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	5	5	theme	tribology	626:634	arg1	results					636:642	The tribology results	622:642	The tribology results	622:642	The tribology results showed that the PEG/CS/GP gel had excellent slow-release properties, especially under pressure, and the PEG played an important role in improving the gel's rheological and mechanical properties.					
29611461	6	6	contain	had	895:897	arg2	effect					918:923	a good lubrication effect	899:923	a good lubrication effect	899:923	Moreover, this study revealed that the release solution had a good lubrication effect because the PEG and GP could crosslink via the hydrogen bond effect.					
29611461	6	6	contain	had	895:897	arg1	solution					886:893	the release solution	874:893	the release solution	874:893	Moreover, this study revealed that the release solution had a good lubrication effect because the PEG and GP could crosslink via the hydrogen bond effect.					
29611461	4	7	theme	glycerophosphate	562:577	arg1	gel					601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel	506:603	gel	601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel was then tested.					
29611461	4	8	theme	/chitosan/sodium	545:560	arg1	gel					601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel	506:603	gel	601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel was then tested.					
29611461	6	9	theme	bond	981:984	arg1	effect					986:991	the hydrogen bond effect	968:991	the hydrogen bond effect	968:991	Moreover, this study revealed that the release solution had a good lubrication effect because the PEG and GP could crosslink via the hydrogen bond effect.					
29611461	2	10	theme	human	379:383	arg1	body					385:388	the human body	375:388	the human body	375:388	However, traditional lubricant would be diluted by body fluids and then absorbed by the human body.					
29611461	5	11	theme	important	762:770	arg1	role					772:775	an important role	759:775	an important role	759:775	The tribology results showed that the PEG/CS/GP gel had excellent slow-release properties, especially under pressure, and the PEG played an important role in improving the gel's rheological and mechanical properties.					
29611461	0	12	theme	Poly	0:3	arg1	gel					55:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel	0:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel	0:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel replaced the joint capsule with slow-release lubricant after joint surgery.					
29611461	1	13	theme	poor	265:268	arg1	ability					282:288	poor lubrication ability	265:288	poor lubrication ability	265:288	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	3	14	theme	injectable	405:414	arg1	gel					416:418	an injectable gel	402:418	an injectable gel with the ability to slow-release lubricant	402:461	Therefore, an injectable gel with the ability to slow-release lubricant was designed to replace the joint capsule.					
29611461	1	15	theme	lubrication	270:280	arg1	ability					282:288	poor lubrication ability	265:288	poor lubrication ability	265:288	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	1	16	theme	replacement	189:199	arg1	surgery					201:207	joint replacement surgery	183:207	joint replacement surgery	183:207	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	0	17	theme	ethylene	5:12	arg1	Poly					0:3	Poly	0:3	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel	0:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel replaced the joint capsule with slow-release lubricant after joint surgery.					
29611461	0	17	theme	ethylene	5:12	arg1	glycol					14:19	ethylene glycol	5:19	ethylene glycol	5:19	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel replaced the joint capsule with slow-release lubricant after joint surgery.					
29611461	4	18	theme	ethylene	529:536	arg1	gel					601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel	506:603	gel	601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel was then tested.					
29611461	0	19	theme	joint	120:124	arg1	surgery					126:132	joint surgery	120:132	joint surgery	120:132	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel replaced the joint capsule with slow-release lubricant after joint surgery.					
29611461	6	20	theme	release	878:884	arg1	solution					886:893	the release solution	874:893	the release solution	874:893	Moreover, this study revealed that the release solution had a good lubrication effect because the PEG and GP could crosslink via the hydrogen bond effect.					
29611461	0	21	theme	/chitosan/sodium	21:36	arg1	gel					55:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel	0:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel	0:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel replaced the joint capsule with slow-release lubricant after joint surgery.					
29611461	5	22	theme	excellent	678:686	arg1	properties					701:710	excellent slow-release properties	678:710	excellent slow-release properties	678:710	The tribology results showed that the PEG/CS/GP gel had excellent slow-release properties, especially under pressure, and the PEG played an important role in improving the gel's rheological and mechanical properties.					
29611461	5	23	theme	slow-release	688:699	arg1	properties					701:710	excellent slow-release properties	678:710	excellent slow-release properties	678:710	The tribology results showed that the PEG/CS/GP gel had excellent slow-release properties, especially under pressure, and the PEG played an important role in improving the gel's rheological and mechanical properties.					
29611461	1	24	theme	joint	183:187	arg1	surgery					201:207	joint replacement surgery	183:207	joint replacement surgery	183:207	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	5	25	theme	mechanical	816:825	arg1	properties					827:836	the gel's rheological and mechanical properties	790:836	properties	827:836	The tribology results showed that the PEG/CS/GP gel had excellent slow-release properties, especially under pressure, and the PEG played an important role in improving the gel's rheological and mechanical properties.					
29611461	6	26	theme	lubrication	906:916	arg1	effect					918:923	a good lubrication effect	899:923	a good lubrication effect	899:923	Moreover, this study revealed that the release solution had a good lubrication effect because the PEG and GP could crosslink via the hydrogen bond effect.					
29611461	4	27	theme	glycol	538:543	arg1	gel					601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel	506:603	gel	601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel was then tested.					
29611461	6	28	theme	good	901:904	arg1	effect					918:923	a good lubrication effect	899:923	a good lubrication effect	899:923	Moreover, this study revealed that the release solution had a good lubrication effect because the PEG and GP could crosslink via the hydrogen bond effect.					
29611461	0	29	theme	glycerophosphate	38:53	arg1	gel					55:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel	0:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel	0:57	Poly(ethylene glycol)/chitosan/sodium glycerophosphate gel replaced the joint capsule with slow-release lubricant after joint surgery.					
29611461	4	30	theme	gel	519:521	arg1	gel					601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel	506:603	gel	601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel was then tested.					
29611461	4	31	dep	gel	601:603	arg1	poly					524:527	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel	506:603	poly	524:527	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel was then tested.					
29611461	4	32	theme	proposed	510:517	arg1	gel					601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel	506:603	gel	601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel was then tested.					
29611461	3	33	with	gel	416:418	arg1	ability					429:435	the ability	425:435	the ability	425:435	Therefore, an injectable gel with the ability to slow-release lubricant was designed to replace the joint capsule.					
29611461	5	34	dep	rheological	800:810	arg1	's					797:798	's	797:798	's	797:798	The tribology results showed that the PEG/CS/GP gel had excellent slow-release properties, especially under pressure, and the PEG played an important role in improving the gel's rheological and mechanical properties.					
29611461	1	35	contain	has	261:263	arg1	fluid					255:259	body fluid	250:259	body fluid	250:259	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	1	35	contain	has	261:263	arg2	ability					282:288	poor lubrication ability	265:288	poor lubrication ability	265:288	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	1	36	theme	wear	214:217	arg1	problems					219:226	wear problems	214:226	wear problems	214:226	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	5	37	theme	PEG/CS/GP	660:668	arg1	gel					670:672	the PEG/CS/GP gel	656:672	the PEG/CS/GP gel	656:672	The tribology results showed that the PEG/CS/GP gel had excellent slow-release properties, especially under pressure, and the PEG played an important role in improving the gel's rheological and mechanical properties.					
29611461	1	38	theme	Body	135:138	arg1	lubricant					167:175	lubricant	167:175	lubricant	167:175	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	1	38	theme	Body	135:138	arg1	fluid					140:144	Body fluid	135:144	Body fluid	135:144	Body fluid is normally the only lubricant after joint replacement surgery, but wear problems have occurred because body fluid has poor lubrication ability.					
29611461	6	39	theme	hydrogen	972:979	arg1	effect					986:991	the hydrogen bond effect	968:991	the hydrogen bond effect	968:991	Moreover, this study revealed that the release solution had a good lubrication effect because the PEG and GP could crosslink via the hydrogen bond effect.					
29611461	3	40	theme	joint	491:495	arg1	capsule					497:503	the joint capsule	487:503	the joint capsule	487:503	Therefore, an injectable gel with the ability to slow-release lubricant was designed to replace the joint capsule.					
29611461	4	41	theme	composite	591:599	arg1	gel					601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel	506:603	gel	601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel was then tested.					
29611461	4	42	theme	PEG/CS/GP	580:588	arg1	gel					601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel	506:603	gel	601:603	The proposed gel, poly(ethylene glycol)/chitosan/sodium glycerophosphate (PEG/CS/GP) composite gel was then tested.					
29371103	4	0	from	chromosome	859:868	arg1	present					921:927	present	921:927	present	921:927	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	0	from	chromosome	859:868	arg1	copy					902:905	only a single copy	888:905	only a single copy of ISApl1	888:915	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	6	1	theme	passage	1250:1256	arg1	days					1242:1245	7 days	1240:1245	7 days of passage	1240:1256	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	7	2	from	resistance	1493:1502	arg1	coli					1510:1513	E. coli	1507:1513	E. coli	1507:1513	These results reveal that the single copy of mcr-1 could result in modification of lipopolysaccharide (LPS) and cause colistin resistance in E. coli.					
29371103	1	3	theme	Escherichia	146:156	arg1	FS13Z2S					172:178	FS13Z2S	172:178	FS13Z2S	172:178	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	3	theme	Escherichia	146:156	arg1	strains					163:169	Two colistin-resistant Escherichia coli strains	123:169	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine	123:249	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	3	theme	Escherichia	146:156	arg1	FS3Z6C					184:189	FS3Z6C	184:189	FS3Z6C	184:189	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	4	4	from	pFS13Z2S	842:849	arg1	present					921:927	present	921:927	present	921:927	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	4	from	pFS13Z2S	842:849	arg1	copy					902:905	only a single copy	888:905	only a single copy of ISApl1	888:915	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	3	5	theme	IS1294-like	810:820	arg1	element					822:828	an IS1294-like element	807:828	an IS1294-like element	807:828	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	4	6	theme	mcr-1	945:949	arg1	cassette					951:958	the mcr-1 cassette	941:958	the mcr-1 cassette	941:958	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	7	7	theme	lipopolysaccharide	1449:1466	arg1	modification					1433:1444	modification	1433:1444	modification of lipopolysaccharide (LPS)	1433:1472	These results reveal that the single copy of mcr-1 could result in modification of lipopolysaccharide (LPS) and cause colistin resistance in E. coli.					
29371103	2	8	dep	revealed	295:302	arg1	whereas					437:443	whereas	437:443	whereas	437:443	Whole-genome sequencing revealed that in strain FS13Z2S mcr-1 occurred in triplicate in the chromosome with another copy encoded on a pHNSHP45-2-like IncHI2 plasmid, whereas in strain FS3Z6C only one copy mcr-1 was inserted in the chromosome.					
29371103	4	9	from	present	921:927	arg1	pFS13Z2S					842:849	plasmid pFS13Z2S	834:849	plasmid pFS13Z2S	834:849	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	9	from	present	921:927	arg1	chromosome					859:868	the chromosome	855:868	the chromosome of strain FS3Z6C	855:885	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	6	10	theme	mcr-1	1324:1328	arg1	copies					1330:1335	the mcr-1 copies	1320:1335	the mcr-1 copies on the chromosome	1320:1353	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	0	11	from	Co-occurrence	0:12	arg1	plasmid					58:64	an IncHI2 plasmid	48:64	an IncHI2 plasmid	48:64	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.					
29371103	0	11	from	Co-occurrence	0:12	arg1	chromosome					30:39	the chromosome	26:39	the chromosome	26:39	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.					
29371103	3	12	theme	mcr-1	577:581	arg1	copies					567:572	chromosomal copies	555:572	chromosomal copies of mcr-1	555:581	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	6	13	theme	mcr-1-harbouring	1147:1162	arg1	unstable					1176:1183	unstable	1176:1183	unstable	1176:1183	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	6	13	theme	mcr-1-harbouring	1147:1162	arg1	plasmid					1164:1170	The mcr-1-harbouring plasmid	1143:1170	The mcr-1-harbouring plasmid	1143:1170	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	3	14	theme	mcr-1	681:685	arg1	cassette					687:694	an mcr-1 cassette	678:694	an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1	678:747	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	3	15	theme	copies	567:572	arg1	triplication					539:550	the triplication	535:550	the triplication of chromosomal copies of mcr-1 in FS13Z2S	535:592	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	3	15	theme	copies	567:572	arg1	due					597:599	due	597:599	due	597:599	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	3	16	theme	insertion	723:731	arg1	ISApl1					742:747	insertion sequence ISApl1	723:747	insertion sequence ISApl1	723:747	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	7	17	theme	single	1396:1401	arg1	copy					1403:1406	the single copy	1392:1406	the single copy of mcr-1	1392:1415	These results reveal that the single copy of mcr-1 could result in modification of lipopolysaccharide (LPS) and cause colistin resistance in E. coli.					
29371103	8	18	theme	host	1653:1656	arg1	strain					1658:1663	the host strain	1649:1663	the host strain	1649:1663	Acquisition of multiple copies of mcr-1, especially on the chromosome, would facilitate stable persistence of colistin resistance in the host strain.					
29371103	0	19	theme	resistance	91:100	arg1	persistence					67:77	persistence	67:77	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.	0:121	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.					
29371103	3	20	theme	sequence	733:740	arg1	ISApl1					742:747	insertion sequence ISApl1	723:747	insertion sequence ISApl1	723:747	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	6	21	theme	Luria-Bertani	1275:1287	arg1	broth					1289:1293	colistin-free Luria-Bertani broth	1261:1293	colistin-free Luria-Bertani broth containing 0.5% SDS	1261:1313	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	6	22	from	copies	1330:1335	arg1	chromosome					1344:1353	the chromosome	1340:1353	the chromosome	1340:1353	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	6	23	theme	wild-type	1188:1196	arg1	FS13Z2S					1205:1211	wild-type strain FS13Z2S	1188:1211	wild-type strain FS13Z2S	1188:1211	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	3	24	contain	containing	667:676	arg2	cassette					687:694	an mcr-1 cassette	678:694	an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1	678:747	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	3	24	contain	containing	667:676	arg1	transposon					656:665	a composite transposon	644:665	a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1	644:747	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	0	25	theme	colistin	82:89	arg1	resistance					91:100	colistin resistance	82:100	colistin resistance	82:100	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.					
29371103	5	26	theme	lipid	1090:1094	arg1	A					1096:1096	lipid A	1090:1096	lipid A	1090:1096	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	8	27	from	persistence	1611:1621	arg1	strain					1658:1663	the host strain	1649:1663	the host strain	1649:1663	Acquisition of multiple copies of mcr-1, especially on the chromosome, would facilitate stable persistence of colistin resistance in the host strain.					
29371103	2	28	theme	Whole-genome	271:282	arg1	sequencing					284:293	Whole-genome sequencing	271:293	Whole-genome sequencing	271:293	Whole-genome sequencing revealed that in strain FS13Z2S mcr-1 occurred in triplicate in the chromosome with another copy encoded on a pHNSHP45-2-like IncHI2 plasmid, whereas in strain FS3Z6C only one copy mcr-1 was inserted in the chromosome.					
29371103	8	29	theme	copies	1540:1545	arg1	Acquisition					1516:1526	Acquisition	1516:1526	Acquisition	1516:1526	Acquisition of multiple copies of mcr-1, especially on the chromosome, would facilitate stable persistence of colistin resistance in the host strain.					
29371103	7	30	theme	mcr-1	1411:1415	arg1	copy					1403:1406	the single copy	1392:1406	the single copy of mcr-1	1392:1415	These results reveal that the single copy of mcr-1 could result in modification of lipopolysaccharide (LPS) and cause colistin resistance in E. coli.					
29371103	1	31	dep	Escherichia	146:156	arg1	coli					158:161	coli	158:161	coli	158:161	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	4	32	theme	strain	873:878	arg1	FS3Z6C					880:885	strain FS3Z6C	873:885	strain FS3Z6C	873:885	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	2	33	theme	strain	448:453	arg1	FS3Z6C					455:460	strain FS3Z6C	448:460	strain FS3Z6C	448:460	Whole-genome sequencing revealed that in strain FS13Z2S mcr-1 occurred in triplicate in the chromosome with another copy encoded on a pHNSHP45-2-like IncHI2 plasmid, whereas in strain FS3Z6C only one copy mcr-1 was inserted in the chromosome.					
29371103	5	34	theme	mcr-1	1136:1140	arg1	number					1126:1131	the copy number	1117:1131	the copy number of mcr-1	1117:1140	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	4	35	theme	plasmid	834:840	arg1	pFS13Z2S					842:849	plasmid pFS13Z2S	834:849	plasmid pFS13Z2S	834:849	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	5	36	theme	inhibitory	1012:1021	arg1	MICs					1039:1042	MICs	1039:1042	MICs	1039:1042	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	5	36	theme	inhibitory	1012:1021	arg1	concentrations					1023:1036	similar colistin minimum inhibitory concentrations	987:1036	similar colistin minimum inhibitory concentrations (MICs)	987:1043	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	6	37	from	unstable	1176:1183	arg1	FS13Z2S					1205:1211	wild-type strain FS13Z2S	1188:1211	wild-type strain FS13Z2S	1188:1211	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	4	38	theme	single	895:900	arg1	present					921:927	present	921:927	present	921:927	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	38	theme	single	895:900	arg1	copy					902:905	only a single copy	888:905	only a single copy of ISApl1	888:915	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	5	39	theme	minimum	1004:1010	arg1	MICs					1039:1042	MICs	1039:1042	MICs	1039:1042	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	5	39	theme	minimum	1004:1010	arg1	concentrations					1023:1036	similar colistin minimum inhibitory concentrations	987:1036	similar colistin minimum inhibitory concentrations (MICs)	987:1043	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	2	40	theme	strain	312:317	arg1	FS13Z2S					319:325	strain FS13Z2S	312:325	strain FS13Z2S	312:325	Whole-genome sequencing revealed that in strain FS13Z2S mcr-1 occurred in triplicate in the chromosome with another copy encoded on a pHNSHP45-2-like IncHI2 plasmid, whereas in strain FS3Z6C only one copy mcr-1 was inserted in the chromosome.					
29371103	0	41	theme	mcr-1	17:21	arg1	Co-occurrence					0:12	Co-occurrence	0:12	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.	0:121	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.					
29371103	3	42	theme	ISApl1	742:747	arg1	copies					713:718	two copies	709:718	two copies of insertion sequence ISApl1	709:747	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	6	43	theme	%	1309:1309	arg1	SDS					1311:1313	0.5% SDS	1306:1313	0.5% SDS	1306:1313	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	7	44	theme	colistin	1484:1491	arg1	resistance					1493:1502	colistin resistance	1484:1502	colistin resistance in E. coli	1484:1513	These results reveal that the single copy of mcr-1 could result in modification of lipopolysaccharide (LPS) and cause colistin resistance in E. coli.					
29371103	3	45	theme	chromosomal	555:565	arg1	copies					567:572	chromosomal copies	555:572	chromosomal copies of mcr-1	555:581	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	5	46	theme	colistin	995:1002	arg1	MICs					1039:1042	MICs	1039:1042	MICs	1039:1042	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	5	46	theme	colistin	995:1002	arg1	concentrations					1023:1036	similar colistin minimum inhibitory concentrations	987:1036	similar colistin minimum inhibitory concentrations (MICs)	987:1043	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	6	47	theme	0.5	1306:1308	arg1	%					1309:1309	%	1309:1309	%	1309:1309	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	3	48	theme	composite	646:654	arg1	transposon					656:665	a composite transposon	644:665	a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1	644:747	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	8	49	theme	resistance	1635:1644	arg1	persistence					1611:1621	stable persistence	1604:1621	stable persistence of colistin resistance in the host strain	1604:1663	Acquisition of multiple copies of mcr-1, especially on the chromosome, would facilitate stable persistence of colistin resistance in the host strain.					
29371103	8	50	theme	stable	1604:1609	arg1	persistence					1611:1621	stable persistence	1604:1621	stable persistence of colistin resistance in the host strain	1604:1663	Acquisition of multiple copies of mcr-1, especially on the chromosome, would facilitate stable persistence of colistin resistance in the host strain.					
29371103	2	51	theme	IncHI2	421:426	arg1	plasmid					428:434	a pHNSHP45-2-like IncHI2 plasmid	403:434	a pHNSHP45-2-like IncHI2 plasmid	403:434	Whole-genome sequencing revealed that in strain FS13Z2S mcr-1 occurred in triplicate in the chromosome with another copy encoded on a pHNSHP45-2-like IncHI2 plasmid, whereas in strain FS3Z6C only one copy mcr-1 was inserted in the chromosome.					
29371103	2	52	theme	copy	471:474	arg1	mcr-1					476:480	only one copy mcr-1	462:480	only one copy mcr-1	462:480	Whole-genome sequencing revealed that in strain FS13Z2S mcr-1 occurred in triplicate in the chromosome with another copy encoded on a pHNSHP45-2-like IncHI2 plasmid, whereas in strain FS3Z6C only one copy mcr-1 was inserted in the chromosome.					
29371103	5	53	theme	featured	1049:1056	arg1	addition					1078:1085	featured phosphoethanolamine addition	1049:1085	featured phosphoethanolamine addition to lipid A	1049:1096	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	5	54	theme	similar	987:993	arg1	MICs					1039:1042	MICs	1039:1042	MICs	1039:1042	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	5	54	theme	similar	987:993	arg1	concentrations					1023:1036	similar colistin minimum inhibitory concentrations	987:1036	similar colistin minimum inhibitory concentrations (MICs)	987:1043	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	2	55	theme	pHNSHP45-2-like	405:419	arg1	plasmid					428:434	a pHNSHP45-2-like IncHI2 plasmid	403:434	a pHNSHP45-2-like IncHI2 plasmid	403:434	Whole-genome sequencing revealed that in strain FS13Z2S mcr-1 occurred in triplicate in the chromosome with another copy encoded on a pHNSHP45-2-like IncHI2 plasmid, whereas in strain FS3Z6C only one copy mcr-1 was inserted in the chromosome.					
29371103	3	56	from	triplication	539:550	arg1	FS13Z2S					586:592	FS13Z2S	586:592	FS13Z2S	586:592	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	5	57	theme	copy	1121:1124	arg1	number					1126:1131	the copy number	1117:1131	the copy number of mcr-1	1117:1140	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	4	58	theme	FS3Z6C	880:885	arg1	pFS13Z2S					842:849	plasmid pFS13Z2S	834:849	plasmid pFS13Z2S	834:849	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	58	theme	FS3Z6C	880:885	arg1	chromosome					859:868	the chromosome	855:868	the chromosome of strain FS3Z6C	855:885	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	5	59	theme	phosphoethanolamine	1058:1076	arg1	addition					1078:1085	featured phosphoethanolamine addition	1049:1085	featured phosphoethanolamine addition to lipid A	1049:1096	The two strains exhibited similar colistin minimum inhibitory concentrations (MICs) and featured phosphoethanolamine addition to lipid A, without regard to the copy number of mcr-1.					
29371103	1	60	dep	strains	163:169	arg1	FS13Z2S					172:178	FS13Z2S	172:178	FS13Z2S	172:178	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	60	dep	strains	163:169	arg1	strains					163:169	Two colistin-resistant Escherichia coli strains	123:169	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine	123:249	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	60	dep	strains	163:169	arg1	FS3Z6C					184:189	FS3Z6C	184:189	FS3Z6C	184:189	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	3	61	theme	pap2	758:761	arg1	gene					763:766	the pap2 gene	754:766	the pap2 gene at the insertion site	754:788	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	3	62	from	site	785:788	arg1	gene					763:766	the pap2 gene	754:766	the pap2 gene at the insertion site	754:788	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	2	63	with	chromosome	363:372	arg1	copy					387:390	another copy	379:390	another copy encoded on a pHNSHP45-2-like IncHI2 plasmid	379:434	Whole-genome sequencing revealed that in strain FS13Z2S mcr-1 occurred in triplicate in the chromosome with another copy encoded on a pHNSHP45-2-like IncHI2 plasmid, whereas in strain FS3Z6C only one copy mcr-1 was inserted in the chromosome.					
29371103	1	64	attach	isolated	231:238	arg2	strains					163:169	Two colistin-resistant Escherichia coli strains	123:169	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine	123:249	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	64	attach	isolated	231:238	arg2	FS3Z6C					184:189	FS3Z6C	184:189	FS3Z6C	184:189	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	64	attach	isolated	231:238	arg1	swine					245:249	swine	245:249	swine	245:249	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	64	attach	isolated	231:238	arg2	FS13Z2S					172:178	FS13Z2S	172:178	FS13Z2S	172:178	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	8	65	theme	mcr-1	1550:1554	arg1	copies					1540:1545	multiple copies	1531:1545	multiple copies of mcr-1	1531:1554	Acquisition of multiple copies of mcr-1, especially on the chromosome, would facilitate stable persistence of colistin resistance in the host strain.					
29371103	6	66	contain	containing	1295:1304	arg2	SDS					1311:1313	0.5% SDS	1306:1313	0.5% SDS	1306:1313	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	6	66	contain	containing	1295:1304	arg1	broth					1289:1293	colistin-free Luria-Bertani broth	1261:1293	colistin-free Luria-Bertani broth containing 0.5% SDS	1261:1313	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	8	67	theme	multiple	1531:1538	arg1	copies					1540:1545	multiple copies	1531:1545	multiple copies of mcr-1	1531:1554	Acquisition of multiple copies of mcr-1, especially on the chromosome, would facilitate stable persistence of colistin resistance in the host strain.					
29371103	6	68	theme	colistin-free	1261:1273	arg1	broth					1289:1293	colistin-free Luria-Bertani broth	1261:1293	colistin-free Luria-Bertani broth containing 0.5% SDS	1261:1313	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	1	69	contain	possessing	192:201	arg1	FS13Z2S					172:178	FS13Z2S	172:178	FS13Z2S	172:178	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	69	contain	possessing	192:201	arg1	strains					163:169	Two colistin-resistant Escherichia coli strains	123:169	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine	123:249	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	69	contain	possessing	192:201	arg1	FS3Z6C					184:189	FS3Z6C	184:189	FS3Z6C	184:189	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	69	contain	possessing	192:201	arg2	mcr-1					225:229	chromosomally encoded mcr-1	203:229	chromosomally encoded mcr-1	203:229	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	3	70	theme	intramolecular	604:617	arg1	events					633:638	intramolecular transposition events	604:638	intramolecular transposition events	604:638	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	4	71	located	present	921:927	arg2	present					921:927	present	921:927	present	921:927	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	71	located	present	921:927	arg1	pFS13Z2S					842:849	plasmid pFS13Z2S	834:849	plasmid pFS13Z2S	834:849	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	71	located	present	921:927	arg1	chromosome					859:868	the chromosome	855:868	the chromosome of strain FS3Z6C	855:885	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	71	located	present	921:927	arg2	copy					902:905	only a single copy	888:905	only a single copy of ISApl1	888:915	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	1	72	theme	encoded	217:223	arg1	mcr-1					225:229	chromosomally encoded mcr-1	203:229	chromosomally encoded mcr-1	203:229	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	6	73	theme	strain	1198:1203	arg1	FS13Z2S					1205:1211	wild-type strain FS13Z2S	1188:1211	wild-type strain FS13Z2S	1188:1211	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	3	74	theme	transposition	619:631	arg1	events					633:638	intramolecular transposition events	604:638	intramolecular transposition events	604:638	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	0	75	theme	IncHI2	51:56	arg1	plasmid					58:64	an IncHI2 plasmid	48:64	an IncHI2 plasmid	48:64	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.					
29371103	0	76	from	persistence	67:77	arg1	coli					117:120	Escherichia coli	105:120	Escherichia coli	105:120	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.					
29371103	8	77	from	Acquisition	1516:1526	arg1	chromosome					1575:1584	the chromosome	1571:1584	the chromosome	1571:1584	Acquisition of multiple copies of mcr-1, especially on the chromosome, would facilitate stable persistence of colistin resistance in the host strain.					
29371103	6	78	from	FS13Z2S	1205:1211	arg1	unstable					1176:1183	unstable	1176:1183	unstable	1176:1183	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	6	78	from	FS13Z2S	1205:1211	arg1	plasmid					1164:1170	The mcr-1-harbouring plasmid	1143:1170	The mcr-1-harbouring plasmid	1143:1170	The mcr-1-harbouring plasmid was unstable in wild-type strain FS13Z2S and was quickly lost after 7 days of passage on colistin-free Luria-Bertani broth containing 0.5% SDS, but the mcr-1 copies on the chromosome persisted.					
29371103	3	79	theme	insertion	775:783	arg1	site					785:788	the insertion site	771:788	the insertion site	771:788	It seems likely that the triplication of chromosomal copies of mcr-1 in FS13Z2S is due to intramolecular transposition events via a composite transposon containing an mcr-1 cassette bracketed by two copies of insertion sequence ISApl1, and the pap2 gene at the insertion site was truncated by an IS1294-like element.					
29371103	0	80	dep	Co-occurrence	0:12	arg1	persistence					67:77	persistence	67:77	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.	0:121	Co-occurrence of mcr-1 in the chromosome and on an IncHI2 plasmid: persistence of colistin resistance in Escherichia coli.					
29371103	4	81	attach	present	921:927	arg1	pFS13Z2S					842:849	plasmid pFS13Z2S	834:849	plasmid pFS13Z2S	834:849	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	81	attach	present	921:927	arg2	copy					902:905	only a single copy	888:905	only a single copy of ISApl1	888:915	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	81	attach	present	921:927	arg1	chromosome					859:868	the chromosome	855:868	the chromosome of strain FS3Z6C	855:885	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	81	attach	present	921:927	arg2	present					921:927	present	921:927	present	921:927	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	1	82	theme	colistin-resistant	127:144	arg1	FS13Z2S					172:178	FS13Z2S	172:178	FS13Z2S	172:178	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	82	theme	colistin-resistant	127:144	arg1	strains					163:169	Two colistin-resistant Escherichia coli strains	123:169	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine	123:249	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	1	82	theme	colistin-resistant	127:144	arg1	FS3Z6C					184:189	FS3Z6C	184:189	FS3Z6C	184:189	Two colistin-resistant Escherichia coli strains (FS13Z2S and FS3Z6C) possessing chromosomally encoded mcr-1 isolated from swine were characterised.					
29371103	8	83	theme	colistin	1626:1633	arg1	resistance					1635:1644	colistin resistance	1626:1644	colistin resistance	1626:1644	Acquisition of multiple copies of mcr-1, especially on the chromosome, would facilitate stable persistence of colistin resistance in the host strain.					
29371103	4	84	theme	ISApl1	910:915	arg1	present					921:927	present	921:927	present	921:927	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
29371103	4	84	theme	ISApl1	910:915	arg1	copy					902:905	only a single copy	888:905	only a single copy of ISApl1	888:915	In plasmid pFS13Z2S and the chromosome of strain FS3Z6C, only a single copy of ISApl1 was present upstream of the mcr-1 cassette.					
30034675	0	0	theme	Cartilaginous	67:79	arg1	Regeneration					81:92	Cartilaginous Regeneration	67:92	Cartilaginous Regeneration	67:92	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	1	1	theme	chitosan	314:321	arg1	association					294:304	the association	290:304	the association of PRP, chitosan, and hydroxyapatite	290:341	The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite.					
30034675	2	2	from	hole	381:384	arg1	animal					394:399	each animal	389:399	each animal	389:399	Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T).					
30034675	8	3	from	interface	1285:1293	arg1	difference					1205:1214	a significant difference	1191:1214	a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface	1191:1293	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	8	3	from	interface	1285:1293	arg1	quantity					1223:1230	the quantity	1219:1230	the quantity of newly formed tissue at the bone/cartilage/implant interface	1219:1293	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	1	4	theme	trochlear	227:235	arg1	bone					237:240	the trochlear bone and cartilaginous regeneration	223:271	bone	237:240	The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite.					
30034675	6	5	theme	moderate	819:826	arg1	reaction					845:852	moderate subchondral bone reaction	819:852	moderate subchondral bone reaction	819:852	The presence of moderate subchondral bone reaction was more frequently reported in group C with 67%.					
30034675	0	6	from	Plasma	14:19	arg1	Bone					58:61	Bone	58:61	Bone	58:61	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	0	6	from	Plasma	14:19	arg1	Regeneration					81:92	Cartilaginous Regeneration	67:92	Cartilaginous Regeneration	67:92	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	0	7	theme	Histomorphometric	154:170	arg1	Evaluations					172:182	Clinical, Radiographic, and Histomorphometric Evaluations	126:182	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.	0:183	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	8	8	from	difference	1205:1214	arg1	interface					1285:1293	the bone/cartilage/implant interface	1258:1293	the bone/cartilage/implant interface	1258:1293	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	8	8	from	difference	1205:1214	arg1	quantity					1223:1230	the quantity	1219:1230	the quantity of newly formed tissue at the bone/cartilage/implant interface	1219:1293	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	8	9	from	groups	1044:1049	arg1	similar					1028:1034	similar	1028:1034	similar	1028:1034	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	8	10	from	similar	1028:1034	arg1	groups					1044:1049	both groups	1039:1049	both groups	1039:1049	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	4	11	theme	group	691:695	arg1	C					697:697	group C	691:697	group C	691:697	Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C.					
30034675	1	12	theme	hydroxyapatite	328:341	arg1	association					294:304	the association	290:304	the association of PRP, chitosan, and hydroxyapatite	290:341	The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite.					
30034675	2	13	theme	group	501:505	arg1	hydroxyapatite					485:498	hydroxyapatite	485:498	hydroxyapatite (group T)	485:508	Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T).					
30034675	2	13	theme	group	501:505	arg1	T					507:507	group T	501:507	group T	501:507	Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T).					
30034675	1	14	theme	cartilaginous	246:258	arg1	regeneration					260:271	the trochlear bone and cartilaginous regeneration	223:271	regeneration	260:271	The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite.					
30034675	0	15	theme	Trochlea	105:112	arg1	Bone					58:61	Bone	58:61	Bone	58:61	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	0	15	theme	Trochlea	105:112	arg1	Regeneration					81:92	Cartilaginous Regeneration	67:92	Cartilaginous Regeneration	67:92	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	0	16	from	Chitosan	42:49	arg1	Bone					58:61	Bone	58:61	Bone	58:61	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	0	16	from	Chitosan	42:49	arg1	Regeneration					81:92	Cartilaginous Regeneration	67:92	Cartilaginous Regeneration	67:92	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	9	17	theme	plasma	1381:1386	arg1	association					1322:1332	the association	1318:1332	the association of chitosan, hydroxyapatite, and platelet-rich plasma	1318:1386	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.					
30034675	9	18	theme	chitosan	1337:1344	arg1	association					1322:1332	the association	1318:1332	the association of chitosan, hydroxyapatite, and platelet-rich plasma	1318:1386	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.					
30034675	5	19	theme	bone	782:785	arg1	reaction					787:794	subchondral bone reaction	770:794	subchondral bone reaction (33%)	770:800	The radiographic evaluation showed that the T group showed absence of subchondral bone reaction (33%).					
30034675	5	19	theme	bone	782:785	arg1	%					799:799	33%	797:799	33%	797:799	The radiographic evaluation showed that the T group showed absence of subchondral bone reaction (33%).					
30034675	0	20	theme	Femoral	97:103	arg1	Trochlea					105:112	Femoral Trochlea	97:112	Femoral Trochlea	97:112	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	4	21	theme	C	697:697	arg1	member					681:686	one member	677:686	one member of group C	677:697	Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C.					
30034675	4	21	theme	C	697:697	arg1	lameness					634:641	lameness	634:641	lameness of two members of the T group	634:671	Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C.					
30034675	2	22	theme	rabbit	361:366	arg1	troches					368:374	rabbit troches	361:374	rabbit troches	361:374	Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T).					
30034675	2	23	dep	empty	410:414	arg1	C					423:423	group C	417:423	group C	417:423	Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T).					
30034675	8	24	theme	bone/cartilage/implant	1262:1283	arg1	interface					1285:1293	the bone/cartilage/implant interface	1258:1293	the bone/cartilage/implant interface	1258:1293	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	7	25	theme	tissue	950:955	arg1	neoformation					957:968	tissue neoformation	950:968	tissue neoformation	950:968	Microscopic evaluation revealed a presence of tissue neoformation, composed of connective tissue.					
30034675	0	26	theme	Platelet-Rich	0:12	arg1	Plasma					14:19	Platelet-Rich Plasma	0:19	Platelet-Rich Plasma	0:19	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	6	27	theme	group	886:890	arg1	C					892:892	group C	886:892	group C	886:892	The presence of moderate subchondral bone reaction was more frequently reported in group C with 67%.					
30034675	6	28	theme	bone	840:843	arg1	reaction					845:852	moderate subchondral bone reaction	819:852	moderate subchondral bone reaction	819:852	The presence of moderate subchondral bone reaction was more frequently reported in group C with 67%.					
30034675	4	29	theme	group	667:671	arg1	members					650:656	two members	646:656	two members of the T group	646:671	Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C.					
30034675	4	30	theme	Clinical-orthopedic	596:614	arg1	evaluation					616:625	Clinical-orthopedic evaluation	596:625	Clinical-orthopedic evaluation	596:625	Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C.					
30034675	2	31	theme	PRP	466:468	arg1	combination					451:461	a combination	449:461	a combination of PRP, chitosan, and hydroxyapatite (group T)	449:508	Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T).					
30034675	3	32	theme	Clinical-orthopedic	511:529	arg1	evaluations					568:578	Clinical-orthopedic, radiographic, and histomorphometric evaluations	511:578	Clinical-orthopedic, radiographic, and histomorphometric evaluations	511:578	Clinical-orthopedic, radiographic, and histomorphometric evaluations were performed.					
30034675	2	33	theme	chitosan	471:478	arg1	combination					451:461	a combination	449:461	a combination of PRP, chitosan, and hydroxyapatite (group T)	449:508	Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T).					
30034675	8	34	theme	tissue	1097:1102	arg1	amount					1077:1082	the amount	1073:1082	the amount of neoformed tissue	1073:1102	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	8	34	theme	tissue	1097:1102	arg1	tissue					1097:1102	neoformed tissue	1087:1102	neoformed tissue	1087:1102	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	0	35	dep	Bone	58:61	arg1	the					54:56	the	54:56	the	54:56	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	9	36	theme	platelet-rich	1367:1379	arg1	plasma					1381:1386	platelet-rich plasma	1367:1386	platelet-rich plasma	1367:1386	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.					
30034675	8	37	theme	neoformed	1087:1095	arg1	tissue					1097:1102	neoformed tissue	1087:1102	neoformed tissue	1087:1102	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	1	38	theme	rabbits	276:282	arg1	bone					237:240	the trochlear bone and cartilaginous regeneration	223:271	bone	237:240	The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite.					
30034675	1	38	theme	rabbits	276:282	arg1	regeneration					260:271	the trochlear bone and cartilaginous regeneration	223:271	regeneration	260:271	The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite.					
30034675	9	39	theme	bone	1396:1399	arg1	healing					1415:1421	bone and cartilage healing	1396:1421	bone and cartilage healing	1396:1421	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.					
30034675	8	40	theme	morphometric	1153:1164	arg1	analysis					1166:1173	the morphometric analysis	1149:1173	the morphometric analysis	1149:1173	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	9	41	theme	cartilage	1405:1413	arg1	healing					1415:1421	bone and cartilage healing	1396:1421	bone and cartilage healing	1396:1421	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.					
30034675	0	42	theme	Clinical	126:133	arg1	Evaluations					172:182	Clinical, Radiographic, and Histomorphometric Evaluations	126:182	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.	0:183	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	5	43	theme	radiographic	704:715	arg1	evaluation					717:726	The radiographic evaluation	700:726	The radiographic evaluation	700:726	The radiographic evaluation showed that the T group showed absence of subchondral bone reaction (33%).					
30034675	9	44	theme	association	1322:1332	arg1	base					1310:1313	The composite base	1296:1313	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma	1296:1386	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.					
30034675	0	45	from	Bone	58:61	arg1	Rabbits					117:123	Rabbits	117:123	Rabbits	117:123	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	5	46	theme	T	744:744	arg1	group					746:750	the T group	740:750	the T group	740:750	The radiographic evaluation showed that the T group showed absence of subchondral bone reaction (33%).					
30034675	5	47	theme	subchondral	770:780	arg1	reaction					787:794	subchondral bone reaction	770:794	subchondral bone reaction (33%)	770:800	The radiographic evaluation showed that the T group showed absence of subchondral bone reaction (33%).					
30034675	5	47	theme	subchondral	770:780	arg1	%					799:799	33%	797:799	33%	797:799	The radiographic evaluation showed that the T group showed absence of subchondral bone reaction (33%).					
30034675	1	48	theme	study	201:205	arg1	aim					189:191	The aim	185:191	The aim of this study	185:205	The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite.					
30034675	2	49	theme	group	417:421	arg1	C					423:423	group C	417:423	group C	417:423	Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T).					
30034675	9	50	theme	hydroxyapatite	1347:1360	arg1	association					1322:1332	the association	1318:1332	the association of chitosan, hydroxyapatite, and platelet-rich plasma	1318:1386	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.					
30034675	0	51	theme	Radiographic	136:147	arg1	Evaluations					172:182	Clinical, Radiographic, and Histomorphometric Evaluations	126:182	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.	0:183	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	3	52	theme	histomorphometric	550:566	arg1	evaluations					568:578	Clinical-orthopedic, radiographic, and histomorphometric evaluations	511:578	Clinical-orthopedic, radiographic, and histomorphometric evaluations	511:578	Clinical-orthopedic, radiographic, and histomorphometric evaluations were performed.					
30034675	6	53	theme	reaction	845:852	arg1	presence					807:814	The presence	803:814	The presence of moderate subchondral bone reaction	803:852	The presence of moderate subchondral bone reaction was more frequently reported in group C with 67%.					
30034675	2	54	theme	hydroxyapatite	485:498	arg1	combination					451:461	a combination	449:461	a combination of PRP, chitosan, and hydroxyapatite (group T)	449:508	Hole was made in rabbit troches, one hole in each animal remained empty (group C), and one was filled by a combination of PRP, chitosan, and hydroxyapatite (group T).					
30034675	7	55	theme	connective	983:992	arg1	tissue					994:999	connective tissue	983:999	connective tissue	983:999	Microscopic evaluation revealed a presence of tissue neoformation, composed of connective tissue.					
30034675	4	56	theme	members	650:656	arg1	member					681:686	one member	677:686	one member of group C	677:697	Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C.					
30034675	4	56	theme	members	650:656	arg1	lameness					634:641	lameness	634:641	lameness of two members of the T group	634:671	Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C.					
30034675	1	57	theme	PRP	309:311	arg1	association					294:304	the association	290:304	the association of PRP, chitosan, and hydroxyapatite	290:341	The aim of this study was to evaluate the trochlear bone and cartilaginous regeneration of rabbits using the association of PRP, chitosan, and hydroxyapatite.					
30034675	8	58	theme	Microscopic	1002:1012	arg1	findings					1014:1021	Microscopic findings	1002:1021	Microscopic findings	1002:1021	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	3	59	theme	radiographic	532:543	arg1	evaluations					568:578	Clinical-orthopedic, radiographic, and histomorphometric evaluations	511:578	Clinical-orthopedic, radiographic, and histomorphometric evaluations	511:578	Clinical-orthopedic, radiographic, and histomorphometric evaluations were performed.					
30034675	7	60	theme	Microscopic	904:914	arg1	evaluation					916:925	Microscopic evaluation	904:925	Microscopic evaluation	904:925	Microscopic evaluation revealed a presence of tissue neoformation, composed of connective tissue.					
30034675	4	61	theme	T	665:665	arg1	group					667:671	the T group	661:671	the T group	661:671	Clinical-orthopedic evaluation showed lameness of two members of the T group and one member of group C.					
30034675	8	62	theme	tissue	1248:1253	arg1	quantity					1223:1230	the quantity	1219:1230	the quantity of newly formed tissue at the bone/cartilage/implant interface	1219:1293	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	8	63	from	difference	1059:1068	arg1	amount					1077:1082	the amount	1073:1082	the amount of neoformed tissue	1073:1102	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	8	63	from	difference	1059:1068	arg1	tissue					1097:1102	neoformed tissue	1087:1102	neoformed tissue	1087:1102	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	8	64	theme	formed	1241:1246	arg1	tissue					1248:1253	newly formed tissue	1235:1253	newly formed tissue	1235:1253	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	8	65	theme	significant	1193:1203	arg1	difference					1205:1214	a significant difference	1191:1214	a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface	1191:1293	Microscopic findings were similar in both groups, with a difference in the amount of neoformed tissue being perceptible, which was confirmed after the morphometric analysis, which revealed a significant difference in the quantity of newly formed tissue at the bone/cartilage/implant interface.					
30034675	9	66	theme	composite	1300:1308	arg1	base					1310:1313	The composite base	1296:1313	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma	1296:1386	The composite base of the association of chitosan, hydroxyapatite, and platelet-rich plasma favored bone and cartilage healing.					
30034675	6	67	theme	subchondral	828:838	arg1	reaction					845:852	moderate subchondral bone reaction	819:852	moderate subchondral bone reaction	819:852	The presence of moderate subchondral bone reaction was more frequently reported in group C with 67%.					
30034675	0	68	from	Regeneration	81:92	arg1	Rabbits					117:123	Rabbits	117:123	Rabbits	117:123	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	0	69	dep	Plasma	14:19	arg1	Evaluations					172:182	Clinical, Radiographic, and Histomorphometric Evaluations	126:182	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.	0:183	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	5	70	theme	reaction	787:794	arg1	absence					759:765	absence	759:765	absence of subchondral bone reaction (33%)	759:800	The radiographic evaluation showed that the T group showed absence of subchondral bone reaction (33%).					
30034675	0	71	from	Hydroxyapatite	22:35	arg1	Bone					58:61	Bone	58:61	Bone	58:61	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	0	71	from	Hydroxyapatite	22:35	arg1	Regeneration					81:92	Cartilaginous Regeneration	67:92	Cartilaginous Regeneration	67:92	Platelet-Rich Plasma, Hydroxyapatite, and Chitosan in the Bone and Cartilaginous Regeneration of Femoral Trochlea in Rabbits: Clinical, Radiographic, and Histomorphometric Evaluations.					
30034675	7	72	theme	neoformation	957:968	arg1	presence					938:945	a presence	936:945	a presence	936:945	Microscopic evaluation revealed a presence of tissue neoformation, composed of connective tissue.					
30878586	2	0	theme	drugs	555:559	arg1	penetration					528:538	the penetration	524:538	the penetration of hydrophobic drugs in the eye	524:570	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	4	1	theme	intraocular	1028:1038	arg1	delivery					1045:1052	intraocular drug delivery	1028:1052	intraocular drug delivery	1028:1052	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	8	2	from	efficacy	1572:1579	arg1	rejection					1608:1616	anti-allograft rejection	1593:1616	anti-allograft rejection	1593:1616	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	4	3	theme	stability	865:873	arg1	terms					856:860	terms	856:860	terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release	856:974	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	4	4	theme	drug	876:879	arg1	loading					881:887	drug loading	876:887	drug loading	876:887	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	0	5	theme	drug	160:163	arg1	delivery					165:172	ocular drug delivery	153:172	ocular drug delivery	153:172	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	5	6	from	NMs	1148:1150	arg1	FK506					1137:1141	FK506	1137:1141	FK506	1137:1141	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	5	6	from	NMs	1148:1150	arg1	amount					1127:1132	the in vitro permeation amount	1103:1132	the in vitro permeation amount of FK506 from NMs	1103:1150	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	4	7	theme	surface	916:922	arg1	tension					924:930	surface tension	916:930	surface tension	916:930	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	7	8	theme	corneal	1390:1396	arg1	transplantation					1398:1412	corneal transplantation	1390:1412	corneal transplantation	1390:1412	Furthermore, the FK506/NH2-PEG-b-PLA/HPMC NMs obviously inhibited the allograft rejection after corneal transplantation in rats.					
30878586	3	9	theme	101.4 ± 1.3 nm	699:712	arg1	diameter					687:694	mean diameter	682:694	mean diameter of 101.4 ± 1.3 nm	682:712	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm were prepared by solvent-evaporation-induced self-assembly in aqueous solution.					
30878586	8	10	theme	FK506/NH2-PEG-b-PLA/HPMC	1438:1461	arg1	formulations					1467:1478	FK506/NH2-PEG-b-PLA/HPMC NMs formulations	1438:1478	FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system	1438:1521	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	6	11	theme	higher	1197:1202	arg1	concentration					1204:1216	the higher concentration	1193:1216	the higher concentration	1193:1216	Besides, the higher concentration and longer retention of FK506 in ocular tissue were also confirmed in vivo.					
30878586	3	12	theme	solvent-evaporation-induced	731:757	arg1	self-assembly					759:771	solvent-evaporation-induced self-assembly	731:771	solvent-evaporation-induced self-assembly in aqueous solution	731:791	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm were prepared by solvent-evaporation-induced self-assembly in aqueous solution.					
30878586	5	13	theme	permeation	1116:1125	arg1	FK506					1137:1141	FK506	1137:1141	FK506	1137:1141	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	5	13	theme	permeation	1116:1125	arg1	amount					1127:1132	the in vitro permeation amount	1103:1132	the in vitro permeation amount of FK506 from NMs	1103:1150	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	4	14	theme	efficiency	904:913	arg1	terms					856:860	terms	856:860	terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release	856:974	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	0	15	theme	hydroxypropyl	119:131	arg1	nanomicelles					21:32	nanomicelles	21:32	nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose	21:147	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	0	15	theme	hydroxypropyl	119:131	arg1	methylcellulose					133:147	hydroxypropyl methylcellulose	119:147	hydroxypropyl methylcellulose	119:147	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	4	16	theme	release	968:974	arg1	terms					856:860	terms	856:860	terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release	856:974	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	8	17	theme	ocular	1495:1500	arg1	system					1516:1521	a promising ocular drug delivery system	1483:1521	a promising ocular drug delivery system	1483:1521	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	7	18	theme	FK506/NH2-PEG-b-PLA/HPMC	1311:1334	arg1	NMs					1336:1338	the FK506/NH2-PEG-b-PLA/HPMC NMs	1307:1338	the FK506/NH2-PEG-b-PLA/HPMC NMs	1307:1338	Furthermore, the FK506/NH2-PEG-b-PLA/HPMC NMs obviously inhibited the allograft rejection after corneal transplantation in rats.					
30878586	6	19	from	concentration	1204:1216	arg1	tissue					1258:1263	ocular tissue	1251:1263	ocular tissue	1251:1263	Besides, the higher concentration and longer retention of FK506 in ocular tissue were also confirmed in vivo.					
30878586	0	20	theme	poly	65:68	arg1	-block-poly					86:96	amino-terminated poly(ethylene glycol)-block-poly	48:96	amino-terminated poly(ethylene glycol)-block-poly(D,L)	48:101	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	8	21	theme	delivery	1507:1514	arg1	system					1516:1521	a promising ocular drug delivery system	1483:1521	a promising ocular drug delivery system	1483:1521	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	0	22	theme	ethylene	70:77	arg1	poly					65:68	poly	65:68	amino-terminated poly(ethylene glycol)-block-poly(D,L)	48:101	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	0	22	theme	ethylene	70:77	arg1	glycol					79:84	ethylene glycol	70:84	ethylene glycol	70:84	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	1	23	theme	drug	309:312	arg1	delivery					314:321	ocular drug delivery	302:321	ocular drug delivery	302:321	FK506 (tacrolimus) is an effective immunosuppressant, but its poor water solubility and low bioavailability impose barriers to ocular drug delivery.					
30878586	8	24	from	bioavailability	1552:1566	arg1	rejection					1608:1616	anti-allograft rejection	1593:1616	anti-allograft rejection	1593:1616	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	4	25	theme	cytotoxicity	942:953	arg1	terms					856:860	terms	856:860	terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release	856:974	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	2	26	theme	drug	588:591	arg1	bioavailability					593:607	the drug bioavailability	584:607	the drug bioavailability for ocular disorder therapy	584:635	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	5	27	theme	%	1077:1077	arg1	drops					1096:1100	the 0.05% FK506 suspension drops	1069:1100	the 0.05% FK506 suspension drops	1069:1100	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	4	28	theme	in	959:960	arg1	release					968:974	in vitro release	959:974	in vitro release	959:974	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	5	29	theme	suspension	1085:1094	arg1	drops					1096:1100	the 0.05% FK506 suspension drops	1069:1100	the 0.05% FK506 suspension drops	1069:1100	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	2	30	theme	D	422:422	arg1	acid					434:437	ethylene glycol-block-poly(D,L)-lactic acid	395:437	acid	434:437	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	30	theme	D	422:422	arg1	poly					390:393	amino-terminated poly	373:393	amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA)	373:454	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	31	theme	disorder	620:627	arg1	therapy					629:635	ocular disorder therapy	613:635	ocular disorder therapy	613:635	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	1	32	theme	poor	237:240	arg1	solubility					248:257	its poor water solubility	233:257	its poor water solubility	233:257	FK506 (tacrolimus) is an effective immunosuppressant, but its poor water solubility and low bioavailability impose barriers to ocular drug delivery.					
30878586	2	33	theme	glycol-block-poly	404:420	arg1	acid					434:437	ethylene glycol-block-poly(D,L)-lactic acid	395:437	acid	434:437	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	33	theme	glycol-block-poly	404:420	arg1	poly					390:393	amino-terminated poly	373:393	amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA)	373:454	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	34	from	penetration	528:538	arg1	eye					568:570	the eye	564:570	the eye	564:570	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	7	35	from	rejection	1374:1382	arg1	rats					1417:1420	rats	1417:1420	rats	1417:1420	Furthermore, the FK506/NH2-PEG-b-PLA/HPMC NMs obviously inhibited the allograft rejection after corneal transplantation in rats.					
30878586	2	36	theme	-lactic	426:432	arg1	acid					434:437	ethylene glycol-block-poly(D,L)-lactic acid	395:437	acid	434:437	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	36	theme	-lactic	426:432	arg1	poly					390:393	amino-terminated poly	373:393	amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA)	373:454	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	37	theme	amino-terminated	373:388	arg1	acid					434:437	ethylene glycol-block-poly(D,L)-lactic acid	395:437	acid	434:437	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	37	theme	amino-terminated	373:388	arg1	NH2-PEG-b-PLA					441:453	NH2-PEG-b-PLA	441:453	NH2-PEG-b-PLA	441:453	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	37	theme	amino-terminated	373:388	arg1	poly					390:393	amino-terminated poly	373:393	amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA)	373:454	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	1	38	theme	low	263:265	arg1	bioavailability					267:281	low bioavailability	263:281	low bioavailability	263:281	FK506 (tacrolimus) is an effective immunosuppressant, but its poor water solubility and low bioavailability impose barriers to ocular drug delivery.					
30878586	8	39	theme	FK506	1584:1588	arg1	efficacy					1572:1579	efficacy	1572:1579	efficacy	1572:1579	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	8	39	theme	FK506	1584:1588	arg1	bioavailability					1552:1566	bioavailability	1552:1566	bioavailability	1552:1566	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	8	40	from	able	1532:1535	arg1	conclusion					1426:1435	conclusion	1426:1435	conclusion	1426:1435	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	2	41	dep	acid	434:437	arg1	L					424:424	ethylene glycol-block-poly(D,L)-lactic acid	395:437	L	424:424	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	0	42	dep	nanomicelles	21:32	arg1	nanomicelles					21:32	nanomicelles	21:32	nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose	21:147	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	0	42	dep	nanomicelles	21:32	arg1	acid					110:113	-lactic acid	102:113	-lactic acid	102:113	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	0	42	dep	nanomicelles	21:32	arg1	methylcellulose					133:147	hydroxypropyl methylcellulose	119:147	hydroxypropyl methylcellulose	119:147	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	8	43	theme	anti-allograft	1593:1606	arg1	rejection					1608:1616	anti-allograft rejection	1593:1616	anti-allograft rejection	1593:1616	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	4	44	theme	drug	1040:1043	arg1	delivery					1045:1052	intraocular drug delivery	1028:1052	intraocular drug delivery	1028:1052	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	0	45	theme	ocular	153:158	arg1	delivery					165:172	ocular drug delivery	153:172	ocular drug delivery	153:172	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	6	46	from	retention	1229:1237	arg1	tissue					1258:1263	ocular tissue	1251:1263	ocular tissue	1251:1263	Besides, the higher concentration and longer retention of FK506 in ocular tissue were also confirmed in vivo.					
30878586	2	47	theme	hydrophobic	543:553	arg1	drugs					555:559	hydrophobic drugs	543:559	hydrophobic drugs	543:559	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	3	48	theme	Spherical	638:646	arg1	NMs					673:675	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs	638:675	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm	638:712	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm were prepared by solvent-evaporation-induced self-assembly in aqueous solution.					
30878586	6	49	theme	ocular	1251:1256	arg1	tissue					1258:1263	ocular tissue	1251:1263	ocular tissue	1251:1263	Besides, the higher concentration and longer retention of FK506 in ocular tissue were also confirmed in vivo.					
30878586	3	50	with	NMs	673:675	arg1	diameter					687:694	mean diameter	682:694	mean diameter of 101.4 ± 1.3 nm	682:712	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm were prepared by solvent-evaporation-induced self-assembly in aqueous solution.					
30878586	6	51	theme	FK506	1242:1246	arg1	concentration					1204:1216	the higher concentration	1193:1216	the higher concentration	1193:1216	Besides, the higher concentration and longer retention of FK506 in ocular tissue were also confirmed in vivo.					
30878586	6	51	theme	FK506	1242:1246	arg1	retention					1229:1237	longer retention	1222:1237	longer retention of FK506 in ocular tissue	1222:1263	Besides, the higher concentration and longer retention of FK506 in ocular tissue were also confirmed in vivo.					
30878586	3	52	theme	mean	682:685	arg1	diameter					687:694	mean diameter	682:694	mean diameter of 101.4 ± 1.3 nm	682:712	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm were prepared by solvent-evaporation-induced self-assembly in aqueous solution.					
30878586	5	53	theme	in	1107:1108	arg1	FK506					1137:1141	FK506	1137:1141	FK506	1137:1141	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	5	53	theme	in	1107:1108	arg1	amount					1127:1132	the in vitro permeation amount	1103:1132	the in vitro permeation amount of FK506 from NMs	1103:1150	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	6	54	theme	longer	1222:1227	arg1	retention					1229:1237	longer retention	1222:1237	longer retention of FK506 in ocular tissue	1222:1263	Besides, the higher concentration and longer retention of FK506 in ocular tissue were also confirmed in vivo.					
30878586	4	55	dep	in	959:960	arg1	vitro					962:966	vitro	962:966	vitro	962:966	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	8	56	from	conclusion	1426:1435	arg1	able					1532:1535	able	1532:1535	able	1532:1535	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	8	57	theme	NMs	1463:1465	arg1	formulations					1467:1478	FK506/NH2-PEG-b-PLA/HPMC NMs formulations	1438:1478	FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system	1438:1521	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	0	58	theme	-lactic	102:108	arg1	nanomicelles					21:32	nanomicelles	21:32	nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose	21:147	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	0	58	theme	-lactic	102:108	arg1	acid					110:113	-lactic acid	102:113	-lactic acid	102:113	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	4	59	theme	tension	924:930	arg1	terms					856:860	terms	856:860	terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release	856:974	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	3	60	theme	aqueous	776:782	arg1	solution					784:791	aqueous solution	776:791	aqueous solution	776:791	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm were prepared by solvent-evaporation-induced self-assembly in aqueous solution.					
30878586	5	61	theme	0.05	1073:1076	arg1	%					1077:1077	%	1077:1077	%	1077:1077	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	4	62	theme	loading	881:887	arg1	terms					856:860	terms	856:860	terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release	856:974	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	8	63	theme	promising	1485:1493	arg1	system					1516:1521	a promising ocular drug delivery system	1483:1521	a promising ocular drug delivery system	1483:1521	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	7	64	theme	allograft	1364:1372	arg1	rejection					1374:1382	the allograft rejection	1360:1382	the allograft rejection after corneal transplantation in rats	1360:1420	Furthermore, the FK506/NH2-PEG-b-PLA/HPMC NMs obviously inhibited the allograft rejection after corneal transplantation in rats.					
30878586	4	65	theme	encapsulation	890:902	arg1	efficiency					904:913	encapsulation efficiency	890:913	encapsulation efficiency	890:913	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	8	66	theme	drug	1502:1505	arg1	system					1516:1521	a promising ocular drug delivery system	1483:1521	a promising ocular drug delivery system	1483:1521	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	8	67	dep	bioavailability	1552:1566	arg1	the					1548:1550	the	1548:1550	the	1548:1550	In conclusion, FK506/NH2-PEG-b-PLA/HPMC NMs formulations as a promising ocular drug delivery system would be able to improve the bioavailability and efficacy of FK506 in anti-allograft rejection.					
30878586	4	68	theme	cellular	933:940	arg1	cytotoxicity					942:953	cellular cytotoxicity	933:953	cellular cytotoxicity	933:953	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	1	69	theme	ocular	302:307	arg1	delivery					314:321	ocular drug delivery	302:321	ocular drug delivery	302:321	FK506 (tacrolimus) is an effective immunosuppressant, but its poor water solubility and low bioavailability impose barriers to ocular drug delivery.					
30878586	3	70	from	self-assembly	759:771	arg1	solution					784:791	aqueous solution	776:791	aqueous solution	776:791	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm were prepared by solvent-evaporation-induced self-assembly in aqueous solution.					
30878586	2	71	theme	hydroxypropyl	460:472	arg1	HPMC					491:494	HPMC	491:494	HPMC	491:494	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	71	theme	hydroxypropyl	460:472	arg1	methylcellulose					474:488	hydroxypropyl methylcellulose	460:488	hydroxypropyl methylcellulose (HPMC)	460:495	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	5	72	theme	FK506	1079:1083	arg1	drops					1096:1100	the 0.05% FK506 suspension drops	1069:1100	the 0.05% FK506 suspension drops	1069:1100	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	0	73	dep	-block-poly	86:96	arg1	L					100:100	L	100:100	L	100:100	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	0	73	dep	-block-poly	86:96	arg1	D					98:98	D	98:98	D	98:98	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	0	74	theme	novel	2:6	arg1	FK506					8:12	A novel FK506	0:12	A novel FK506	0:12	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	5	75	dep	in	1107:1108	arg1	vitro					1110:1114	vitro	1110:1114	vitro	1110:1114	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	2	76	theme	ocular	613:618	arg1	therapy					629:635	ocular disorder therapy	613:635	ocular disorder therapy	613:635	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	77	theme	NMs	342:344	arg1	formulations					347:358	The nanomicelles (NMs) formulations	324:358	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC)	324:495	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	4	78	dep	evaluated	843:851	arg1	NMs					798:800	The NMs	794:800	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs	794:1008	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	4	78	dep	evaluated	843:851	arg1	suitable					1015:1022	suitable	1015:1022	suitable	1015:1022	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	1	79	theme	water	242:246	arg1	solubility					248:257	its poor water solubility	233:257	its poor water solubility	233:257	FK506 (tacrolimus) is an effective immunosuppressant, but its poor water solubility and low bioavailability impose barriers to ocular drug delivery.					
30878586	2	80	theme	ethylene	395:402	arg1	acid					434:437	ethylene glycol-block-poly(D,L)-lactic acid	395:437	acid	434:437	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	2	80	theme	ethylene	395:402	arg1	poly					390:393	amino-terminated poly	373:393	amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA)	373:454	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	5	81	theme	significant	1162:1172	arg1	increase					1174:1181	significant increase	1162:1181	significant increase	1162:1181	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	0	82	theme	amino-terminated	48:63	arg1	-block-poly					86:96	amino-terminated poly(ethylene glycol)-block-poly	48:96	amino-terminated poly(ethylene glycol)-block-poly(D,L)	48:101	A novel FK506 loaded nanomicelles consisting of amino-terminated poly(ethylene glycol)-block-poly(D,L)-lactic acid and hydroxypropyl methylcellulose for ocular drug delivery.					
30878586	4	83	theme	solubilized	820:830	arg1	FK506					832:836	sufficiently solubilized FK506	807:836	sufficiently solubilized FK506	807:836	The NMs that sufficiently solubilized FK506 were evaluated in terms of stability, drug loading, encapsulation efficiency, surface tension, cellular cytotoxicity and in vitro release, and the results revealed the NMs were suitable for intraocular drug delivery.					
30878586	5	84	theme	FK506	1137:1141	arg1	FK506					1137:1141	FK506	1137:1141	FK506	1137:1141	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	5	84	theme	FK506	1137:1141	arg1	amount					1127:1132	the in vitro permeation amount	1103:1132	the in vitro permeation amount of FK506 from NMs	1103:1150	Compared with the 0.05% FK506 suspension drops, the in vitro permeation amount of FK506 from NMs exhibited significant increase.					
30878586	3	85	theme	FK506/NH2-PEG-b-PLA/HPMC	648:671	arg1	NMs					673:675	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs	638:675	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm	638:712	Spherical FK506/NH2-PEG-b-PLA/HPMC NMs with mean diameter of 101.4 ± 1.3 nm were prepared by solvent-evaporation-induced self-assembly in aqueous solution.					
30878586	2	86	theme	nanomicelles	328:339	arg1	formulations					347:358	The nanomicelles (NMs) formulations	324:358	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC)	324:495	The nanomicelles (NMs) formulations comprised of amino-terminated poly(ethylene glycol-block-poly(D,L)-lactic acid) (NH2-PEG-b-PLA) and hydroxypropyl methylcellulose (HPMC) were developed to increase the penetration of hydrophobic drugs in the eye and enhance the drug bioavailability for ocular disorder therapy.					
30878586	1	87	theme	effective	200:208	arg1	FK506					175:179	FK506	175:179	FK506 (tacrolimus)	175:192	FK506 (tacrolimus) is an effective immunosuppressant, but its poor water solubility and low bioavailability impose barriers to ocular drug delivery.					
30878586	1	87	theme	effective	200:208	arg1	immunosuppressant					210:226	an effective immunosuppressant	197:226	an effective immunosuppressant	197:226	FK506 (tacrolimus) is an effective immunosuppressant, but its poor water solubility and low bioavailability impose barriers to ocular drug delivery.					
31751691	0	0	theme	infections	110:119	arg1	treatment					78:86	treatment	78:86	treatment of multiple bacterial infections	78:119	Fortified hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections.					
31751691	9	1	theme	anaerobic	1296:1304	arg1	species					1316:1322	the tested aerobic and anaerobic bacterial species	1273:1322	the tested aerobic and anaerobic bacterial species	1273:1322	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	5	2	theme	PEG-CS	554:559	arg1	efficient					712:720	efficient	712:720	efficient	712:720	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	2	theme	PEG-CS	554:559	arg1	activity					528:535	the antibacterial activity	510:535	the antibacterial activity of the resulting PEG-CS	510:559	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	6	3	theme	inflammatory	894:905	arg1	response					907:914	any concomitant inflammatory response	878:914	any concomitant inflammatory response	878:914	Montmorillonite nanoclay (MMT) was prepared and used to encapsulate ibuprofen (IBU) as anti-inflammatory drug to reduce any concomitant inflammatory response during bacterial infection.					
31751691	9	4	from	activity	1261:1268	arg1	species					1316:1322	the tested aerobic and anaerobic bacterial species	1273:1322	the tested aerobic and anaerobic bacterial species	1273:1322	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	0	5	theme	bacterial	100:108	arg1	infections					110:119	multiple bacterial infections	91:119	multiple bacterial infections	91:119	Fortified hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections.					
31751691	9	6	theme	bacterial	1306:1314	arg1	species					1316:1322	the tested aerobic and anaerobic bacterial species	1273:1322	the tested aerobic and anaerobic bacterial species	1273:1322	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	5	7	theme	silver	677:682	arg1	nanoparticles					684:696	silver nanoparticles	677:696	silver nanoparticles	677:696	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	7	8	theme	IBU-MMT	998:1004	arg1	nanocomplex					1006:1016	IBU-MMT nanocomplex	998:1016	PEG-HBCS-AgNPs as well as IBU-MMT nanocomplex	972:1016	The successful synthesis of PEG-HBCS-AgNPs as well as IBU-MMT nanocomplex was confirmed using FTIR, 1H NMR, DSC, TGA and EDX.					
31751691	9	9	theme	CS-based	1209:1216	arg1	formulation					1222:1232	the newly developed drugs-loaded CS-based NIM formulation	1176:1232	the newly developed drugs-loaded CS-based NIM formulation	1176:1232	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	5	10	with	conjugation	579:589	arg1	HB					636:637	HB	636:637	HB	636:637	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	10	with	conjugation	579:589	arg1	AgNPs					699:703	AgNPs	699:703	AgNPs	699:703	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	10	with	conjugation	579:589	arg1	hyperbranches					621:633	dendritic polyamidoamine hyperbranches	596:633	dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs)	596:704	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	10	with	conjugation	579:589	arg1	immobilization					659:672	in-situ immobilization	651:672	dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs)	596:704	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	3	11	theme	multiple	380:387	arg1	therapeutics					403:414	multiple antibacterial therapeutics	380:414	multiple antibacterial therapeutics	380:414	This study proposes a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time.					
31751691	9	12	theme	NIM	1218:1220	arg1	formulation					1222:1232	the newly developed drugs-loaded CS-based NIM formulation	1176:1232	the newly developed drugs-loaded CS-based NIM formulation	1176:1232	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	5	13	theme	nanoparticles	684:696	arg1	HB					636:637	HB	636:637	HB	636:637	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	13	theme	nanoparticles	684:696	arg1	AgNPs					699:703	AgNPs	699:703	AgNPs	699:703	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	13	theme	nanoparticles	684:696	arg1	hyperbranches					621:633	dendritic polyamidoamine hyperbranches	596:633	dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs)	596:704	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	13	theme	nanoparticles	684:696	arg1	immobilization					659:672	in-situ immobilization	651:672	dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs)	596:704	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	7	14	theme	nanocomplex	1006:1016	arg1	synthesis					959:967	The successful synthesis	944:967	The successful synthesis of PEG-HBCS-AgNPs as well as IBU-MMT nanocomplex	944:1016	The successful synthesis of PEG-HBCS-AgNPs as well as IBU-MMT nanocomplex was confirmed using FTIR, 1H NMR, DSC, TGA and EDX.					
31751691	3	15	theme	antibacterial	389:401	arg1	therapeutics					403:414	multiple antibacterial therapeutics	380:414	multiple antibacterial therapeutics	380:414	This study proposes a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time.					
31751691	6	16	theme	bacterial	923:931	arg1	infection					933:941	bacterial infection	923:941	bacterial infection	923:941	Montmorillonite nanoclay (MMT) was prepared and used to encapsulate ibuprofen (IBU) as anti-inflammatory drug to reduce any concomitant inflammatory response during bacterial infection.					
31751691	9	17	theme	developed	1186:1194	arg1	formulation					1222:1232	the newly developed drugs-loaded CS-based NIM formulation	1176:1232	the newly developed drugs-loaded CS-based NIM formulation	1176:1232	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	4	18	theme	water	486:490	arg1	solubility					492:501	its limited water solubility	474:501	its limited water solubility	474:501	Chitosan (CS) was PEGylated to overcome its limited water solubility.					
31751691	9	19	theme	drugs-loaded	1196:1207	arg1	formulation					1222:1232	the newly developed drugs-loaded CS-based NIM formulation	1176:1232	the newly developed drugs-loaded CS-based NIM formulation	1176:1232	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	9	20	theme	aerobic	1284:1290	arg1	species					1316:1322	the tested aerobic and anaerobic bacterial species	1273:1322	the tested aerobic and anaerobic bacterial species	1273:1322	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	7	21	theme	PEG-HBCS-AgNPs	972:985	arg1	synthesis					959:967	The successful synthesis	944:967	The successful synthesis of PEG-HBCS-AgNPs as well as IBU-MMT nanocomplex	944:1016	The successful synthesis of PEG-HBCS-AgNPs as well as IBU-MMT nanocomplex was confirmed using FTIR, 1H NMR, DSC, TGA and EDX.					
31751691	5	22	theme	antibacterial	514:526	arg1	efficient					712:720	efficient	712:720	efficient	712:720	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	22	theme	antibacterial	514:526	arg1	activity					528:535	the antibacterial activity	510:535	the antibacterial activity of the resulting PEG-CS	510:559	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	0	23	theme	hyperbranched	10:22	arg1	composites					63:72	hyperbranched PEGylated chitosan-based nano-in-micro composites	10:72	hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections	10:119	Fortified hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections.					
31751691	3	24	theme	antibacterial	340:352	arg1	platform					354:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform	289:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time	289:431	This study proposes a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time.					
31751691	5	25	theme	in-situ	651:657	arg1	AgNPs					699:703	AgNPs	699:703	AgNPs	699:703	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	25	theme	in-situ	651:657	arg1	immobilization					659:672	in-situ immobilization	651:672	dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs)	596:704	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	26	theme	dendritic	596:604	arg1	HB					636:637	HB	636:637	HB	636:637	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	26	theme	dendritic	596:604	arg1	hyperbranches					621:633	dendritic polyamidoamine hyperbranches	596:633	dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs)	596:704	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	6	27	used	used	806:809	arg2	MMT					784:786	MMT	784:786	MMT	784:786	Montmorillonite nanoclay (MMT) was prepared and used to encapsulate ibuprofen (IBU) as anti-inflammatory drug to reduce any concomitant inflammatory response during bacterial infection.					
31751691	6	27	used	used	806:809	arg2	nanoclay					774:781	Montmorillonite nanoclay	758:781	Montmorillonite nanoclay (MMT)	758:787	Montmorillonite nanoclay (MMT) was prepared and used to encapsulate ibuprofen (IBU) as anti-inflammatory drug to reduce any concomitant inflammatory response during bacterial infection.					
31751691	3	28	theme	same	423:426	arg1	time					428:431	the same time	419:431	the same time	419:431	This study proposes a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time.					
31751691	8	29	theme	SEM	1070:1072	arg1	micrographs					1074:1084	SEM micrographs	1070:1084	SEM micrographs	1070:1084	SEM micrographs showed a complete formation of NIM spherical particles with a size around 13 µm.					
31751691	5	30	theme	polyamidoamine	606:619	arg1	HB					636:637	HB	636:637	HB	636:637	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	5	30	theme	polyamidoamine	606:619	arg1	hyperbranches					621:633	dendritic polyamidoamine hyperbranches	596:633	dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs)	596:704	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	1	31	theme	Bacterial	122:130	arg1	threat					153:158	a real threat	146:158	a real threat to human health	146:174	Bacterial resistance is a real threat to human health.					
31751691	1	31	theme	Bacterial	122:130	arg1	resistance					132:141	Bacterial resistance	122:141	Bacterial resistance	122:141	Bacterial resistance is a real threat to human health.					
31751691	0	32	theme	chitosan-based	34:47	arg1	composites					63:72	hyperbranched PEGylated chitosan-based nano-in-micro composites	10:72	hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections	10:119	Fortified hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections.					
31751691	9	33	theme	widespread	1250:1259	arg1	activity					1261:1268	a better widespread activity	1241:1268	a better widespread activity on the tested aerobic and anaerobic bacterial species	1241:1322	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	0	34	theme	PEGylated	24:32	arg1	composites					63:72	hyperbranched PEGylated chitosan-based nano-in-micro composites	10:72	hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections	10:119	Fortified hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections.					
31751691	3	35	theme	new	291:293	arg1	platform					354:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform	289:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time	289:431	This study proposes a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time.					
31751691	2	36	theme	common	193:198	arg1	strategies					200:209	the most common strategies	184:209	the most common strategies used to overcome this problem	184:239	One of the most common strategies used to overcome this problem is the combination therapy.					
31751691	9	37	theme	further	1353:1359	arg1	optimization					1361:1372	further optimization	1353:1372	further optimization	1353:1372	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	5	38	theme	multiple	730:737	arg1	strains					749:755	multiple bacterial strains	730:755	multiple bacterial strains	730:755	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	0	39	theme	nano-in-micro	49:61	arg1	composites					63:72	hyperbranched PEGylated chitosan-based nano-in-micro composites	10:72	hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections	10:119	Fortified hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections.					
31751691	5	40	theme	bacterial	739:747	arg1	strains					749:755	multiple bacterial strains	730:755	multiple bacterial strains	730:755	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	4	41	theme	limited	478:484	arg1	solubility					492:501	its limited water solubility	474:501	its limited water solubility	474:501	Chitosan (CS) was PEGylated to overcome its limited water solubility.					
31751691	8	42	theme	NIM	1117:1119	arg1	particles					1131:1139	NIM spherical particles	1117:1139	NIM spherical particles	1117:1139	SEM micrographs showed a complete formation of NIM spherical particles with a size around 13 µm.					
31751691	6	43	theme	concomitant	882:892	arg1	response					907:914	any concomitant inflammatory response	878:914	any concomitant inflammatory response	878:914	Montmorillonite nanoclay (MMT) was prepared and used to encapsulate ibuprofen (IBU) as anti-inflammatory drug to reduce any concomitant inflammatory response during bacterial infection.					
31751691	8	44	theme	particles	1131:1139	arg1	formation					1104:1112	a complete formation	1093:1112	a complete formation of NIM spherical particles with a size around 13 µm	1093:1164	SEM micrographs showed a complete formation of NIM spherical particles with a size around 13 µm.					
31751691	8	45	theme	complete	1095:1102	arg1	formation					1104:1112	a complete formation	1093:1112	a complete formation of NIM spherical particles with a size around 13 µm	1093:1164	SEM micrographs showed a complete formation of NIM spherical particles with a size around 13 µm.					
31751691	3	46	theme	NIMs	334:337	arg1	platform					354:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform	289:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time	289:431	This study proposes a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time.					
31751691	1	47	theme	real	148:151	arg1	threat					153:158	a real threat	146:158	a real threat to human health	146:174	Bacterial resistance is a real threat to human health.					
31751691	1	47	theme	real	148:151	arg1	resistance					132:141	Bacterial resistance	122:141	Bacterial resistance	122:141	Bacterial resistance is a real threat to human health.					
31751691	8	48	with	formation	1104:1112	arg1	size					1148:1151	a size	1146:1151	a size around 13 µm	1146:1164	SEM micrographs showed a complete formation of NIM spherical particles with a size around 13 µm.					
31751691	9	49	theme	promising	1377:1385	arg1	approach					1387:1394	a promising approach	1375:1394	a promising approach for overcoming multiple-bacterial infection	1375:1438	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	3	50	theme	chitosan-based	295:308	arg1	platform					354:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform	289:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time	289:431	This study proposes a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time.					
31751691	6	51	theme	anti-inflammatory	845:861	arg1	drug					863:866	anti-inflammatory drug	845:866	anti-inflammatory drug	845:866	Montmorillonite nanoclay (MMT) was prepared and used to encapsulate ibuprofen (IBU) as anti-inflammatory drug to reduce any concomitant inflammatory response during bacterial infection.					
31751691	7	52	theme	1H	1044:1045	arg1	NMR					1047:1049	1H NMR	1044:1049	1H NMR	1044:1049	The successful synthesis of PEG-HBCS-AgNPs as well as IBU-MMT nanocomplex was confirmed using FTIR, 1H NMR, DSC, TGA and EDX.					
31751691	7	53	theme	successful	948:957	arg1	synthesis					959:967	The successful synthesis	944:967	The successful synthesis of PEG-HBCS-AgNPs as well as IBU-MMT nanocomplex	944:1016	The successful synthesis of PEG-HBCS-AgNPs as well as IBU-MMT nanocomplex was confirmed using FTIR, 1H NMR, DSC, TGA and EDX.					
31751691	3	54	theme	nano-in-microparticles	310:331	arg1	platform					354:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform	289:361	a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time	289:431	This study proposes a new chitosan-based nano-in-microparticles (NIMs) antibacterial platform that can deliver multiple antibacterial therapeutics at the same time.					
31751691	9	55	theme	tested	1277:1282	arg1	species					1316:1322	the tested aerobic and anaerobic bacterial species	1273:1322	the tested aerobic and anaerobic bacterial species	1273:1322	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31751691	0	56	theme	multiple	91:98	arg1	infections					110:119	multiple bacterial infections	91:119	multiple bacterial infections	91:119	Fortified hyperbranched PEGylated chitosan-based nano-in-micro composites for treatment of multiple bacterial infections.					
31751691	2	57	theme	strategies	200:209	arg1	therapy					260:266	the combination therapy	244:266	the combination therapy	244:266	One of the most common strategies used to overcome this problem is the combination therapy.					
31751691	2	57	theme	strategies	200:209	arg1	strategies					200:209	the most common strategies	184:209	the most common strategies used to overcome this problem	184:239	One of the most common strategies used to overcome this problem is the combination therapy.					
31751691	2	57	theme	strategies	200:209	arg1	One					177:179	One	177:179	One	177:179	One of the most common strategies used to overcome this problem is the combination therapy.					
31751691	6	58	theme	Montmorillonite	758:772	arg1	MMT					784:786	MMT	784:786	MMT	784:786	Montmorillonite nanoclay (MMT) was prepared and used to encapsulate ibuprofen (IBU) as anti-inflammatory drug to reduce any concomitant inflammatory response during bacterial infection.					
31751691	6	58	theme	Montmorillonite	758:772	arg1	nanoclay					774:781	Montmorillonite nanoclay	758:781	Montmorillonite nanoclay (MMT)	758:787	Montmorillonite nanoclay (MMT) was prepared and used to encapsulate ibuprofen (IBU) as anti-inflammatory drug to reduce any concomitant inflammatory response during bacterial infection.					
31751691	1	59	theme	human	163:167	arg1	health					169:174	human health	163:174	human health	163:174	Bacterial resistance is a real threat to human health.					
31751691	2	60	theme	combination	248:258	arg1	therapy					260:266	the combination therapy	244:266	the combination therapy	244:266	One of the most common strategies used to overcome this problem is the combination therapy.					
31751691	2	60	theme	combination	248:258	arg1	strategies					200:209	the most common strategies	184:209	the most common strategies used to overcome this problem	184:239	One of the most common strategies used to overcome this problem is the combination therapy.					
31751691	2	60	theme	combination	248:258	arg1	One					177:179	One	177:179	One	177:179	One of the most common strategies used to overcome this problem is the combination therapy.					
31751691	5	61	theme	resulting	544:552	arg1	PEG-CS					554:559	the resulting PEG-CS	540:559	the resulting PEG-CS	540:559	Then, the antibacterial activity of the resulting PEG-CS was fortified via conjugation with dendritic polyamidoamine hyperbranches (HB) as well as in-situ immobilization of silver nanoparticles (AgNPs) to be efficient against multiple bacterial strains.					
31751691	8	62	theme	spherical	1121:1129	arg1	particles					1131:1139	NIM spherical particles	1117:1139	NIM spherical particles	1117:1139	SEM micrographs showed a complete formation of NIM spherical particles with a size around 13 µm.					
31751691	9	63	theme	multiple-bacterial	1411:1428	arg1	infection					1430:1438	multiple-bacterial infection	1411:1438	multiple-bacterial infection	1411:1438	Besides, the newly developed drugs-loaded CS-based NIM formulation showed a better widespread activity on the tested aerobic and anaerobic bacterial species, and it may represent, after further optimization, a promising approach for overcoming multiple-bacterial infection.					
31790805	10	0	theme	minor	1733:1737	arg1	effects					1739:1745	minor effects	1733:1745	minor effects that were mainly seen in case of protein restriction	1733:1798	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	6	1	from	flies	908:912	arg1	terms					968:972	terms	968:972	terms of development	968:987	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	10	2	from	changes	1588:1594	arg1	factors					1607:1613	dietary factors	1599:1613	dietary factors	1599:1613	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	8	3	theme	Other	1261:1265	arg1	reserves					1289:1296	energy reserves	1282:1296	energy reserves	1282:1296	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance were affected by diet alone.					
31790805	8	3	theme	Other	1261:1265	arg1	rate					1309:1312	metabolic rate	1299:1312	metabolic rate	1299:1312	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance were affected by diet alone.					
31790805	8	3	theme	Other	1261:1265	arg1	preference					1320:1329	food preference	1315:1329	food preference	1315:1329	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance were affected by diet alone.					
31790805	8	3	theme	Other	1261:1265	arg1	traits					1267:1272	Other traits	1261:1272	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance	1261:1354	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance were affected by diet alone.					
31790805	8	3	theme	Other	1261:1265	arg1	tolerance					1346:1354	starvation tolerance	1335:1354	starvation tolerance	1335:1354	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance were affected by diet alone.					
31790805	4	4	theme	bacterial	753:761	arg1	load					763:766	the bacterial load	749:766	the bacterial load	749:766	In each of these diets, we compared flies with conventional vs. artificially altered gut microbiota with antibiotics that reduced the bacterial load.					
31790805	9	5	theme	yeast-poor	1451:1460	arg1	diets					1462:1466	yeast-poor diets	1451:1466	yeast-poor diets	1451:1466	When detected, the effect of antibiotic treatment was stronger in yeast-poor diets, suggesting that gut bacterial community might help to counterbalance nutritional deficiencies.					
31790805	7	6	theme	lesser	1155:1160	arg1	extent					1162:1167	a lesser extent	1153:1167	a lesser extent	1153:1167	Diets, and to a lesser extent antibiotic treatment, affected development rate, weight, and cold tolerance of adult flies.					
31790805	3	7	dep	diets	388:392	arg1	three					395:399	three	395:399	three	395:399	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	0	8	with	interaction	80:90	arg1	microbiota					101:110	gut microbiota	97:110	gut microbiota	97:110	Dietary nutrient balance shapes phenotypic traits of Drosophila melanogaster in interaction with gut microbiota.					
31790805	3	9	dep	content	462:468	arg1	2:2					471:473	2:2	471:473	2:2	471:473	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	3	9	dep	content	462:468	arg1	16:16					481:485	16:16	481:485	16:16	481:485	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	3	9	dep	content	462:468	arg1	8:8					476:478	8:8	476:478	8:8	476:478	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	10	10	theme	development	1672:1682	arg1	re-organization					1632:1646	a global re-organization	1623:1646	a global re-organization of fly's physiology and development	1623:1682	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	0	11	theme	gut	97:99	arg1	microbiota					101:110	gut microbiota	97:110	gut microbiota	97:110	Dietary nutrient balance shapes phenotypic traits of Drosophila melanogaster in interaction with gut microbiota.					
31790805	9	12	theme	bacterial	1489:1497	arg1	community					1499:1507	gut bacterial community	1485:1507	gut bacterial community	1485:1507	When detected, the effect of antibiotic treatment was stronger in yeast-poor diets, suggesting that gut bacterial community might help to counterbalance nutritional deficiencies.					
31790805	5	13	theme	yeast	858:862	arg1	amount					843:848	the amount	839:848	the amount of live yeast associated with the flies	839:888	The antibiotic treatment also had the surprising effect of increasing the amount of live yeast associated with the flies.					
31790805	5	13	theme	yeast	858:862	arg1	yeast					858:862	live yeast	853:862	live yeast	853:862	The antibiotic treatment also had the surprising effect of increasing the amount of live yeast associated with the flies.					
31790805	7	14	dep	Diets	1139:1143	arg1	treatment					1180:1188	antibiotic treatment	1169:1188	antibiotic treatment	1169:1188	Diets, and to a lesser extent antibiotic treatment, affected development rate, weight, and cold tolerance of adult flies.					
31790805	6	15	theme	food	1001:1004	arg1	flies					908:912	flies	908:912	flies from these ten treatments (5 diets × 2 microbiota) in terms of development	908:987	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	15	theme	food	1001:1004	arg1	preference					1006:1015	food preference	1001:1015	food preference	1001:1015	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	2	16	theme	indirect	285:292	arg1	consequences					294:305	indirect consequences	285:305	indirect consequences for the host	285:318	Furthermore, diet can affect gut microbiota composition and abundance, with indirect consequences for the host.					
31790805	10	17	contain	had	1729:1731	arg1	manipulation					1694:1705	the manipulation	1690:1705	the manipulation of gut microorganisms	1690:1727	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	10	17	contain	had	1729:1731	arg2	effects					1739:1745	minor effects	1733:1745	minor effects that were mainly seen in case of protein restriction	1733:1798	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	4	18	theme	altered	696:702	arg1	microbiota					708:717	conventional vs. artificially altered gut microbiota	666:717	conventional vs. artificially altered gut microbiota	666:717	In each of these diets, we compared flies with conventional vs. artificially altered gut microbiota with antibiotics that reduced the bacterial load.					
31790805	6	19	from	treatments	929:938	arg1	mass					995:998	body mass	990:998	body mass	990:998	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	19	from	treatments	929:938	arg1	rate					1043:1046	metabolic rate	1033:1046	metabolic rate	1033:1046	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	19	from	treatments	929:938	arg1	flies					908:912	flies	908:912	flies from these ten treatments (5 diets × 2 microbiota) in terms of development	908:987	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	19	from	treatments	929:938	arg1	preference					1006:1015	food preference	1001:1015	food preference	1001:1015	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	19	from	treatments	929:938	arg1	reserves					1023:1030	body reserves	1018:1030	body reserves	1018:1030	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	19	from	treatments	929:938	arg1	range					1054:1058	a range	1052:1058	a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance)	1052:1136	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	3	20	theme	weight	491:496	arg1	%					498:498	weight %	491:498	weight %	491:498	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	8	21	theme	energy	1282:1287	arg1	reserves					1289:1296	energy reserves	1282:1296	energy reserves	1282:1296	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance were affected by diet alone.					
31790805	6	22	dep	traits	1080:1085	arg1	traits					1080:1085	stress tolerance traits	1063:1085	stress tolerance traits (heat, cold, starvation and desiccation tolerance)	1063:1136	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	22	dep	traits	1080:1085	arg1	starvation					1100:1109	starvation	1100:1109	starvation	1100:1109	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	22	dep	traits	1080:1085	arg1	tolerance					1127:1135	desiccation tolerance	1115:1135	desiccation tolerance	1115:1135	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	22	dep	traits	1080:1085	arg1	cold					1094:1097	cold	1094:1097	cold	1094:1097	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	22	dep	traits	1080:1085	arg1	heat					1088:1091	heat	1088:1091	heat	1088:1091	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	0	23	theme	nutrient	8:15	arg1	balance					17:23	Dietary nutrient balance	0:23	Dietary nutrient balance	0:23	Dietary nutrient balance shapes phenotypic traits of Drosophila melanogaster in interaction with gut microbiota.					
31790805	8	24	theme	starvation	1335:1344	arg1	tolerance					1346:1354	starvation tolerance	1335:1354	starvation tolerance	1335:1354	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance were affected by diet alone.					
31790805	9	25	theme	gut	1485:1487	arg1	community					1499:1507	gut bacterial community	1485:1507	gut bacterial community	1485:1507	When detected, the effect of antibiotic treatment was stronger in yeast-poor diets, suggesting that gut bacterial community might help to counterbalance nutritional deficiencies.					
31790805	10	26	theme	protein	1780:1786	arg1	restriction					1788:1798	protein restriction	1780:1798	protein restriction	1780:1798	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	7	27	theme	cold	1230:1233	arg1	tolerance					1235:1243	cold tolerance	1230:1243	cold tolerance of adult flies	1230:1258	Diets, and to a lesser extent antibiotic treatment, affected development rate, weight, and cold tolerance of adult flies.					
31790805	8	28	theme	metabolic	1299:1307	arg1	rate					1309:1312	metabolic rate	1299:1312	metabolic rate	1299:1312	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance were affected by diet alone.					
31790805	0	29	theme	Dietary	0:6	arg1	balance					17:23	Dietary nutrient balance	0:23	Dietary nutrient balance	0:23	Dietary nutrient balance shapes phenotypic traits of Drosophila melanogaster in interaction with gut microbiota.					
31790805	4	30	with	flies	655:659	arg1	microbiota					708:717	conventional vs. artificially altered gut microbiota	666:717	conventional vs. artificially altered gut microbiota	666:717	In each of these diets, we compared flies with conventional vs. artificially altered gut microbiota with antibiotics that reduced the bacterial load.					
31790805	9	31	theme	treatment	1425:1433	arg1	effect					1404:1409	the effect	1400:1409	the effect of antibiotic treatment	1400:1433	When detected, the effect of antibiotic treatment was stronger in yeast-poor diets, suggesting that gut bacterial community might help to counterbalance nutritional deficiencies.					
31790805	9	31	theme	treatment	1425:1433	arg1	stronger					1439:1446	stronger	1439:1446	stronger	1439:1446	When detected, the effect of antibiotic treatment was stronger in yeast-poor diets, suggesting that gut bacterial community might help to counterbalance nutritional deficiencies.					
31790805	3	32	from	2:2	471:473	arg1	%					498:498	weight %	491:498	weight %	491:498	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	6	33	theme	body	1018:1021	arg1	flies					908:912	flies	908:912	flies from these ten treatments (5 diets × 2 microbiota) in terms of development	908:987	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	33	theme	body	1018:1021	arg1	reserves					1023:1030	body reserves	1018:1030	body reserves	1018:1030	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	3	34	theme	yeast	421:425	arg1	ratio					427:431	balanced sugar:yeast ratio	406:431	balanced sugar:yeast ratio	406:431	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	7	35	theme	development	1200:1210	arg1	rate					1212:1215	development rate	1200:1215	development rate	1200:1215	Diets, and to a lesser extent antibiotic treatment, affected development rate, weight, and cold tolerance of adult flies.					
31790805	7	36	theme	antibiotic	1169:1178	arg1	treatment					1180:1188	antibiotic treatment	1169:1188	antibiotic treatment	1169:1188	Diets, and to a lesser extent antibiotic treatment, affected development rate, weight, and cold tolerance of adult flies.					
31790805	6	37	theme	tolerance	1070:1078	arg1	traits					1080:1085	stress tolerance traits	1063:1085	stress tolerance traits (heat, cold, starvation and desiccation tolerance)	1063:1136	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	37	theme	tolerance	1070:1078	arg1	starvation					1100:1109	starvation	1100:1109	starvation	1100:1109	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	37	theme	tolerance	1070:1078	arg1	tolerance					1127:1135	desiccation tolerance	1115:1135	desiccation tolerance	1115:1135	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	37	theme	tolerance	1070:1078	arg1	cold					1094:1097	cold	1094:1097	cold	1094:1097	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	37	theme	tolerance	1070:1078	arg1	heat					1088:1091	heat	1088:1091	heat	1088:1091	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	4	38	theme	gut	704:706	arg1	microbiota					708:717	conventional vs. artificially altered gut microbiota	666:717	conventional vs. artificially altered gut microbiota	666:717	In each of these diets, we compared flies with conventional vs. artificially altered gut microbiota with antibiotics that reduced the bacterial load.					
31790805	9	39	theme	antibiotic	1414:1423	arg1	treatment					1425:1433	antibiotic treatment	1414:1433	antibiotic treatment	1414:1433	When detected, the effect of antibiotic treatment was stronger in yeast-poor diets, suggesting that gut bacterial community might help to counterbalance nutritional deficiencies.					
31790805	3	40	theme	different	378:386	arg1	diets					388:392	five different diets	373:392	five different diets; three with balanced sugar:yeast ratio	373:431	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	6	41	theme	metabolic	1033:1041	arg1	flies					908:912	flies	908:912	flies from these ten treatments (5 diets × 2 microbiota) in terms of development	908:987	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	41	theme	metabolic	1033:1041	arg1	rate					1043:1046	metabolic rate	1033:1046	metabolic rate	1033:1046	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	3	42	theme	caloric	454:460	arg1	content					462:468	caloric content	454:468	caloric content (2:2, 8:8, 16:16, in weight %)	454:499	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	3	43	theme	balanced	406:413	arg1	ratio					427:431	balanced sugar:yeast ratio	406:431	balanced sugar:yeast ratio	406:431	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	5	44	theme	surprising	807:816	arg1	effect					818:823	the surprising effect	803:823	the surprising effect of increasing the amount of live yeast associated with the flies	803:888	The antibiotic treatment also had the surprising effect of increasing the amount of live yeast associated with the flies.					
31790805	6	45	theme	traits	1080:1085	arg1	mass					995:998	body mass	990:998	body mass	990:998	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	45	theme	traits	1080:1085	arg1	rate					1043:1046	metabolic rate	1033:1046	metabolic rate	1033:1046	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	45	theme	traits	1080:1085	arg1	flies					908:912	flies	908:912	flies from these ten treatments (5 diets × 2 microbiota) in terms of development	908:987	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	45	theme	traits	1080:1085	arg1	preference					1006:1015	food preference	1001:1015	food preference	1001:1015	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	45	theme	traits	1080:1085	arg1	reserves					1023:1030	body reserves	1018:1030	body reserves	1018:1030	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	45	theme	traits	1080:1085	arg1	range					1054:1058	a range	1052:1058	a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance)	1052:1136	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	3	46	theme	high	571:574	arg1	content					582:588	high yeast content	571:588	high yeast content	571:588	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	2	47	theme	microbiota	242:251	arg1	composition					253:263	gut microbiota composition	238:263	gut microbiota composition	238:263	Furthermore, diet can affect gut microbiota composition and abundance, with indirect consequences for the host.					
31790805	6	48	theme	diets × 2	943:951	arg1	microbiota					953:962	5 diets × 2 microbiota	941:962	5 diets × 2 microbiota	941:962	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	48	theme	diets × 2	943:951	arg1	treatments					929:938	these ten treatments	919:938	these ten treatments (5 diets × 2 microbiota)	919:963	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	10	49	theme	dietary	1599:1605	arg1	factors					1607:1613	dietary factors	1599:1613	dietary factors	1599:1613	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	0	50	theme	phenotypic	32:41	arg1	traits					43:48	phenotypic traits	32:48	phenotypic traits of Drosophila melanogaster	32:75	Dietary nutrient balance shapes phenotypic traits of Drosophila melanogaster in interaction with gut microbiota.					
31790805	3	51	theme	sugar	415:419	arg1	ratio					427:431	balanced sugar:yeast ratio	406:431	balanced sugar:yeast ratio	406:431	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	3	52	theme	low	557:559	arg1	sugar					561:565	low sugar	557:565	low sugar	557:565	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	3	52	theme	low	557:559	arg1	2:16					591:594	2:16	591:594	2:16	591:594	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	3	53	theme	yeast	532:536	arg1	ratio					538:542	imbalanced sugar:yeast ratio	515:542	imbalanced sugar:yeast ratio	515:542	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	10	54	theme	physiology	1657:1666	arg1	re-organization					1632:1646	a global re-organization	1623:1646	a global re-organization of fly's physiology and development	1623:1682	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	3	55	theme	imbalanced	515:524	arg1	ratio					538:542	imbalanced sugar:yeast ratio	515:542	imbalanced sugar:yeast ratio	515:542	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	1	56	theme	dietary	117:123	arg1	composition					134:144	The dietary nutrient composition	113:144	The dietary nutrient composition	113:144	The dietary nutrient composition can affect insects' phenotypes by modulating their physiology.					
31790805	8	57	theme	food	1315:1318	arg1	preference					1320:1329	food preference	1315:1329	food preference	1315:1329	Other traits such as energy reserves, metabolic rate, food preference, or starvation tolerance were affected by diet alone.					
31790805	9	58	theme	nutritional	1538:1548	arg1	deficiencies					1550:1561	nutritional deficiencies	1538:1561	nutritional deficiencies	1538:1561	When detected, the effect of antibiotic treatment was stronger in yeast-poor diets, suggesting that gut bacterial community might help to counterbalance nutritional deficiencies.					
31790805	10	59	theme	gut	1710:1712	arg1	microorganisms					1714:1727	gut microorganisms	1710:1727	gut microorganisms	1710:1727	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	3	60	theme	sugar	526:530	arg1	ratio					538:542	imbalanced sugar:yeast ratio	515:542	imbalanced sugar:yeast ratio	515:542	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	10	61	theme	microorganisms	1714:1727	arg1	manipulation					1694:1705	the manipulation	1690:1705	the manipulation of gut microorganisms	1690:1727	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	1	62	theme	nutrient	125:132	arg1	composition					134:144	The dietary nutrient composition	113:144	The dietary nutrient composition	113:144	The dietary nutrient composition can affect insects' phenotypes by modulating their physiology.					
31790805	7	63	theme	flies	1254:1258	arg1	rate					1212:1215	development rate	1200:1215	development rate	1200:1215	Diets, and to a lesser extent antibiotic treatment, affected development rate, weight, and cold tolerance of adult flies.					
31790805	7	63	theme	flies	1254:1258	arg1	tolerance					1235:1243	cold tolerance	1230:1243	cold tolerance of adult flies	1230:1258	Diets, and to a lesser extent antibiotic treatment, affected development rate, weight, and cold tolerance of adult flies.					
31790805	7	63	theme	flies	1254:1258	arg1	weight					1218:1223	weight	1218:1223	weight	1218:1223	Diets, and to a lesser extent antibiotic treatment, affected development rate, weight, and cold tolerance of adult flies.					
31790805	6	64	theme	stress	1063:1068	arg1	traits					1080:1085	stress tolerance traits	1063:1085	stress tolerance traits (heat, cold, starvation and desiccation tolerance)	1063:1136	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	64	theme	stress	1063:1068	arg1	starvation					1100:1109	starvation	1100:1109	starvation	1100:1109	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	64	theme	stress	1063:1068	arg1	tolerance					1127:1135	desiccation tolerance	1115:1135	desiccation tolerance	1115:1135	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	64	theme	stress	1063:1068	arg1	cold					1094:1097	cold	1094:1097	cold	1094:1097	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	64	theme	stress	1063:1068	arg1	heat					1088:1091	heat	1088:1091	heat	1088:1091	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	4	65	theme	conventional	666:677	arg1	microbiota					708:717	conventional vs. artificially altered gut microbiota	666:717	conventional vs. artificially altered gut microbiota	666:717	In each of these diets, we compared flies with conventional vs. artificially altered gut microbiota with antibiotics that reduced the bacterial load.					
31790805	2	66	theme	gut	238:240	arg1	composition					253:263	gut microbiota composition	238:263	gut microbiota composition	238:263	Furthermore, diet can affect gut microbiota composition and abundance, with indirect consequences for the host.					
31790805	7	67	theme	adult	1248:1252	arg1	flies					1254:1258	adult flies	1248:1258	adult flies	1248:1258	Diets, and to a lesser extent antibiotic treatment, affected development rate, weight, and cold tolerance of adult flies.					
31790805	3	68	theme	yeast	576:580	arg1	content					582:588	high yeast content	571:588	high yeast content	571:588	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	5	69	theme	live	853:856	arg1	yeast					858:862	live yeast	853:862	live yeast	853:862	The antibiotic treatment also had the surprising effect of increasing the amount of live yeast associated with the flies.					
31790805	5	70	contain	had	799:801	arg2	effect					818:823	the surprising effect	803:823	the surprising effect of increasing the amount of live yeast associated with the flies	803:888	The antibiotic treatment also had the surprising effect of increasing the amount of live yeast associated with the flies.					
31790805	5	70	contain	had	799:801	arg1	treatment					784:792	The antibiotic treatment	769:792	The antibiotic treatment	769:792	The antibiotic treatment also had the surprising effect of increasing the amount of live yeast associated with the flies.					
31790805	6	71	theme	body	990:993	arg1	mass					995:998	body mass	990:998	body mass	990:998	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	71	theme	body	990:993	arg1	flies					908:912	flies	908:912	flies from these ten treatments (5 diets × 2 microbiota) in terms of development	908:987	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	3	72	dep	increasing	443:452	arg1	either					545:550	either	545:550	either	545:550	In this study, we reared Drosophila melanogaster on five different diets; three with balanced sugar:yeast ratio, but with increasing caloric content (2:2, 8:8, 16:16, in weight %), and two with imbalanced sugar:yeast ratio, either with low sugar and high yeast content (2:16) or vice-versa (16:2).					
31790805	5	73	theme	antibiotic	773:782	arg1	treatment					784:792	The antibiotic treatment	769:792	The antibiotic treatment	769:792	The antibiotic treatment also had the surprising effect of increasing the amount of live yeast associated with the flies.					
31790805	0	74	theme	melanogaster	64:75	arg1	traits					43:48	phenotypic traits	32:48	phenotypic traits of Drosophila melanogaster	32:75	Dietary nutrient balance shapes phenotypic traits of Drosophila melanogaster in interaction with gut microbiota.					
31790805	10	75	theme	global	1625:1630	arg1	re-organization					1632:1646	a global re-organization	1623:1646	a global re-organization of fly's physiology and development	1623:1682	These results show that changes in dietary factors lead to a global re-organization of fly's physiology and development while the manipulation of gut microorganisms had minor effects that were mainly seen in case of protein restriction.					
31790805	6	76	theme	development	977:987	arg1	terms					968:972	terms	968:972	terms of development	968:987	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	77	theme	desiccation	1115:1125	arg1	traits					1080:1085	stress tolerance traits	1063:1085	stress tolerance traits (heat, cold, starvation and desiccation tolerance)	1063:1136	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
31790805	6	77	theme	desiccation	1115:1125	arg1	tolerance					1127:1135	desiccation tolerance	1115:1135	desiccation tolerance	1115:1135	We characterized flies from these ten treatments (5 diets × 2 microbiota) in terms of development, body mass, food preference, body reserves, metabolic rate and a range of stress tolerance traits (heat, cold, starvation and desiccation tolerance).					
30292547	0	0	theme	pre-crystallized	112:127	arg1	powders					139:145	pre-crystallized acid whey powders	112:145	pre-crystallized acid whey powders	112:145	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.					
30292547	8	1	theme	new	1450:1452	arg1	information					1454:1464	new information	1450:1464	new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW	1450:1591	These results provide new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW.					
30292547	6	2	theme	network	1107:1113	arg1	presence					1087:1094	the presence	1083:1094	the presence of a loose network of protein aggregates (≤10 µm)	1083:1144	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	8	3	from	information	1454:1464	arg1	useful					1541:1546	useful	1541:1546	useful	1541:1546	These results provide new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW.					
30292547	8	3	from	information	1454:1464	arg1	changes					1524:1530	the composition- and process-dependent physicochemical changes	1469:1530	the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW	1469:1591	These results provide new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW.					
30292547	3	4	theme	final	677:681	arg1	powders					683:689	the final powders	673:689	the final powders from 2 independent industrial-scale trials	673:732	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	1	5	theme	liquid	170:175	arg1	co-product					177:186	the liquid co-product	166:186	the liquid co-product arising from acid-induced precipitation of casein from skim milk	166:251	Acid whey (AW) is the liquid co-product arising from acid-induced precipitation of casein from skim milk.					
30292547	1	5	theme	liquid	170:175	arg1	whey					153:156	Acid whey	148:156	Acid whey (AW)	148:161	Acid whey (AW) is the liquid co-product arising from acid-induced precipitation of casein from skim milk.					
30292547	5	6	theme	greater	932:938	arg1	extent					940:945	a greater extent	930:945	a greater extent of lactose crystallization	930:972	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	0	7	theme	whey	134:137	arg1	powders					139:145	pre-crystallized acid whey powders	112:145	pre-crystallized acid whey powders	112:145	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.					
30292547	3	8	theme	powders	683:689	arg1	microstructure					655:668	the microstructure	651:668	the microstructure of the final powders from 2 independent industrial-scale trials	651:732	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	5	9	theme	crystallization	958:972	arg1	extent					940:945	a greater extent	930:945	a greater extent of lactose crystallization	930:972	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	5	9	theme	crystallization	958:972	arg1	ratio					1002:1006	a higher ash-to-protein ratio	978:1006	a higher ash-to-protein ratio in that concentrate	978:1026	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	5	10	theme	lactose	950:956	arg1	crystallization					958:972	lactose crystallization	950:972	lactose crystallization	950:972	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	0	11	theme	acid	129:132	arg1	powders					139:145	pre-crystallized acid whey powders	112:145	pre-crystallized acid whey powders	112:145	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.					
30292547	6	12	theme	lactose	1150:1156	arg1	crystals					1158:1165	lactose crystals	1150:1165	lactose crystals (100-300 µm)	1150:1178	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	6	12	theme	lactose	1150:1156	arg1	µm					1176:1177	100-300 µm	1168:1177	100-300 µm	1168:1177	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	5	13	from	solidification	881:894	arg1	former					903:908	former	903:908	former	903:908	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	7	14	theme	scanning	1284:1291	arg1	microscopy					1302:1311	scanning electron microscopy	1284:1311	scanning electron microscopy	1284:1311	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	3	15	from	precipitation	533:545	arg1	concentrates					610:621	liquid AW concentrates	600:621	liquid AW concentrates (∼55% total solids)	600:641	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	3	15	from	precipitation	533:545	arg1	solids					635:640	∼55% total solids	624:640	∼55% total solids	624:640	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	7	16	theme	particles	1342:1350	arg1	surface					1321:1327	the surface	1317:1327	the surface of AW powder particles	1317:1350	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	7	17	theme	AW	1332:1333	arg1	particles					1342:1350	AW powder particles	1332:1350	AW powder particles	1332:1350	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	0	18	theme	powders	139:145	arg1	manufacture					97:107	industrial-scale manufacture	80:107	industrial-scale manufacture of pre-crystallized acid whey powders	80:145	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.					
30292547	3	19	theme	independent	698:708	arg1	trials					727:732	2 independent industrial-scale trials	696:732	2 independent industrial-scale trials	696:732	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	3	20	theme	protein	548:554	arg1	aggregation					556:566	protein aggregation	548:566	protein aggregation	548:566	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	6	21	theme	loose	1101:1105	arg1	network					1107:1113	a loose network	1099:1113	a loose network of protein aggregates (≤10 µm)	1099:1144	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	7	22	theme	powder	1335:1340	arg1	particles					1342:1350	AW powder particles	1332:1350	AW powder particles	1332:1350	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	2	23	theme	subsequent	450:459	arg1	crystallization					469:483	subsequent lactose crystallization	450:483	subsequent lactose crystallization	450:483	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	3	24	theme	industrial-scale	710:725	arg1	trials					727:732	2 independent industrial-scale trials	696:732	2 independent industrial-scale trials	696:732	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	4	25	theme	concentrate	853:863	arg1	storage					818:824	storage	818:824	storage (24 h) of pre-crystallized concentrate	818:863	These AW concentrates were observed to solidify either during processing or during storage (24 h) of pre-crystallized concentrate.					
30292547	4	25	theme	concentrate	853:863	arg1	h					830:830	24 h	827:830	24 h	827:830	These AW concentrates were observed to solidify either during processing or during storage (24 h) of pre-crystallized concentrate.					
30292547	4	26	theme	AW	741:742	arg1	concentrates					744:755	These AW concentrates	735:755	These AW concentrates	735:755	These AW concentrates were observed to solidify either during processing or during storage (24 h) of pre-crystallized concentrate.					
30292547	5	27	from	ratio	1002:1006	arg1	concentrate					1016:1026	that concentrate	1011:1026	that concentrate	1011:1026	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	0	28	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.	0:146	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.					
30292547	5	29	theme	higher	980:985	arg1	ratio					1002:1006	a higher ash-to-protein ratio	978:1006	a higher ash-to-protein ratio in that concentrate	978:1026	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	4	30	theme	pre-crystallized	836:851	arg1	concentrate					853:863	pre-crystallized concentrate	836:863	pre-crystallized concentrate	836:863	These AW concentrates were observed to solidify either during processing or during storage (24 h) of pre-crystallized concentrate.					
30292547	2	31	theme	mineral	316:322	arg1	content					324:330	its high mineral content	307:330	its high mineral content	307:330	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	1	32	theme	acid-induced	201:212	arg1	precipitation					214:226	acid-induced precipitation	201:226	acid-induced precipitation of casein from skim milk	201:251	Acid whey (AW) is the liquid co-product arising from acid-induced precipitation of casein from skim milk.					
30292547	2	33	theme	concentrate	431:441	arg1	viscosity					407:415	high viscosity	402:415	high viscosity of the liquid concentrate	402:441	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	3	34	theme	∼55	624:626	arg1	%					627:627	%	627:627	%	627:627	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	6	35	theme	AW	1218:1219	arg1	concentrate					1221:1231	the solidified AW concentrate	1203:1231	the solidified AW concentrate	1203:1231	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	2	36	theme	high	311:314	arg1	content					324:330	its high mineral content	307:330	its high mineral content	307:330	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	7	37	theme	insoluble	1381:1389	arg1	phosphate					1399:1407	insoluble calcium phosphate	1381:1407	insoluble calcium phosphate	1381:1407	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	2	38	theme	liquid	424:429	arg1	concentrate					431:441	the liquid concentrate	420:441	the liquid concentrate	420:441	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	8	39	theme	physicochemical	1508:1522	arg1	useful					1541:1546	useful	1541:1546	useful	1541:1546	These results provide new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW.					
30292547	8	39	theme	physicochemical	1508:1522	arg1	changes					1524:1530	the composition- and process-dependent physicochemical changes	1469:1530	the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW	1469:1591	These results provide new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW.					
30292547	3	40	theme	lactose	573:579	arg1	crystallization					581:595	lactose crystallization	573:595	lactose crystallization	573:595	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	3	41	theme	mineral	525:531	arg1	precipitation					533:545	mineral precipitation	525:545	mineral precipitation	525:545	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	2	42	theme	drying	489:494	arg1	steps					496:500	drying steps	489:500	drying steps	489:500	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	6	43	theme	microscopy	1053:1062	arg1	analysis					1064:1071	Confocal laser scanning microscopy analysis	1029:1071	Confocal laser scanning microscopy analysis	1029:1071	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	2	44	theme	Further	254:260	arg1	processing					262:271	Further processing	254:271	Further processing of AW	254:277	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	5	45	theme	rapid	875:879	arg1	solidification					881:894	The more rapid solidification	866:894	The more rapid solidification in the former	866:908	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	1	46	theme	casein	231:236	arg1	precipitation					214:226	acid-induced precipitation	201:226	acid-induced precipitation of casein from skim milk	201:251	Acid whey (AW) is the liquid co-product arising from acid-induced precipitation of casein from skim milk.					
30292547	6	47	theme	laser	1038:1042	arg1	microscopy					1053:1062	Confocal laser scanning microscopy	1029:1062	Confocal laser scanning microscopy analysis	1029:1071	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	8	48	theme	composition-	1473:1484	arg1	useful					1541:1546	useful	1541:1546	useful	1541:1546	These results provide new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW.					
30292547	8	48	theme	composition-	1473:1484	arg1	changes					1524:1530	the composition- and process-dependent physicochemical changes	1469:1530	the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW	1469:1591	These results provide new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW.					
30292547	0	49	theme	Multi-component	21:35	arg1	interactions					37:48	Multi-component interactions	21:48	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.	0:146	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.					
30292547	2	50	theme	high	402:405	arg1	viscosity					407:415	high viscosity	402:415	high viscosity of the liquid concentrate	402:441	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	3	51	from	microstructure	655:668	arg1	trials					727:732	2 independent industrial-scale trials	696:732	2 independent industrial-scale trials	696:732	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	3	52	theme	liquid	600:605	arg1	concentrates					610:621	liquid AW concentrates	600:621	liquid AW concentrates (∼55% total solids)	600:641	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	3	52	theme	liquid	600:605	arg1	solids					635:640	∼55% total solids	624:640	∼55% total solids	624:640	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	6	53	theme	protein	1118:1124	arg1	aggregates					1126:1135	protein aggregates	1118:1135	protein aggregates (≤10 µm)	1118:1144	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	6	53	theme	protein	1118:1124	arg1	µm					1142:1143	≤10 µm	1138:1143	≤10 µm	1138:1143	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	1	54	theme	Acid	148:151	arg1	co-product					177:186	the liquid co-product	166:186	the liquid co-product arising from acid-induced precipitation of casein from skim milk	166:251	Acid whey (AW) is the liquid co-product arising from acid-induced precipitation of casein from skim milk.					
30292547	1	54	theme	Acid	148:151	arg1	AW					159:160	AW	159:160	AW	159:160	Acid whey (AW) is the liquid co-product arising from acid-induced precipitation of casein from skim milk.					
30292547	1	54	theme	Acid	148:151	arg1	whey					153:156	Acid whey	148:156	Acid whey (AW)	148:161	Acid whey (AW) is the liquid co-product arising from acid-induced precipitation of casein from skim milk.					
30292547	2	55	theme	lactose	461:467	arg1	crystallization					469:483	subsequent lactose crystallization	450:483	subsequent lactose crystallization	450:483	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	8	56	theme	process-dependent	1490:1506	arg1	useful					1541:1546	useful	1541:1546	useful	1541:1546	These results provide new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW.					
30292547	8	56	theme	process-dependent	1490:1506	arg1	changes					1524:1530	the composition- and process-dependent physicochemical changes	1469:1530	the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW	1469:1591	These results provide new information on the composition- and process-dependent physicochemical changes that are useful in designing and optimizing processes for AW.					
30292547	3	57	theme	AW	607:608	arg1	concentrates					610:621	liquid AW concentrates	600:621	liquid AW concentrates (∼55% total solids)	600:641	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	3	57	theme	AW	607:608	arg1	solids					635:640	∼55% total solids	624:640	∼55% total solids	624:640	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	7	58	theme	electron	1293:1300	arg1	microscopy					1302:1311	scanning electron microscopy	1284:1311	scanning electron microscopy	1284:1311	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	5	59	theme	ash-to-protein	987:1000	arg1	ratio					1002:1006	a higher ash-to-protein ratio	978:1006	a higher ash-to-protein ratio in that concentrate	978:1026	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	3	60	theme	total	629:633	arg1	concentrates					610:621	liquid AW concentrates	600:621	liquid AW concentrates (∼55% total solids)	600:641	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	3	60	theme	total	629:633	arg1	solids					635:640	∼55% total solids	624:640	∼55% total solids	624:640	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	7	61	theme	calcium	1391:1397	arg1	phosphate					1399:1407	insoluble calcium phosphate	1381:1407	insoluble calcium phosphate	1381:1407	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	7	62	theme	phosphate	1399:1407	arg1	formation					1368:1376	the formation	1364:1376	the formation of insoluble calcium phosphate	1364:1407	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	1	63	theme	skim	243:246	arg1	milk					248:251	skim milk	243:251	skim milk	243:251	Acid whey (AW) is the liquid co-product arising from acid-induced precipitation of casein from skim milk.					
30292547	1	64	from	milk	248:251	arg1	precipitation					214:226	acid-induced precipitation	201:226	acid-induced precipitation of casein from skim milk	201:251	Acid whey (AW) is the liquid co-product arising from acid-induced precipitation of casein from skim milk.					
30292547	6	65	theme	solidified	1207:1216	arg1	concentrate					1221:1231	the solidified AW concentrate	1203:1231	the solidified AW concentrate	1203:1231	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	2	66	theme	AW	276:277	arg1	processing					262:271	Further processing	254:271	Further processing of AW	254:277	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	7	67	from	surface	1321:1327	arg1	evident					1269:1275	evident	1269:1275	evident	1269:1275	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	6	68	theme	aggregates	1126:1135	arg1	network					1107:1113	a loose network	1099:1113	a loose network of protein aggregates (≤10 µm)	1099:1144	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	6	69	theme	Confocal	1029:1036	arg1	microscopy					1053:1062	Confocal laser scanning microscopy	1029:1062	Confocal laser scanning microscopy analysis	1029:1071	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	7	70	theme	Mineral-based	1234:1246	arg1	precipitate					1248:1258	Mineral-based precipitate	1234:1258	Mineral-based precipitate	1234:1258	Mineral-based precipitate was also evident, using scanning electron microscopy, at the surface of AW powder particles, indicating the formation of insoluble calcium phosphate during processing.					
30292547	6	71	theme	scanning	1044:1051	arg1	microscopy					1053:1062	Confocal laser scanning microscopy	1029:1062	Confocal laser scanning microscopy analysis	1029:1071	Confocal laser scanning microscopy analysis indicated the presence of a loose network of protein aggregates (≤10 µm) and lactose crystals (100-300 µm) distributed throughout the solidified AW concentrate.					
30292547	0	72	dep	communication	6:18	arg1	interactions					37:48	Multi-component interactions	21:48	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.	0:146	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.					
30292547	2	73	theme	proteins	371:378	arg1	aggregation					351:361	aggregation	351:361	aggregation	351:361	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	5	74	from	extent	940:945	arg1	concentrate					1016:1026	that concentrate	1011:1026	that concentrate	1011:1026	The more rapid solidification in the former was associated with a greater extent of lactose crystallization and a higher ash-to-protein ratio in that concentrate.					
30292547	0	75	theme	industrial-scale	80:95	arg1	manufacture					97:107	industrial-scale manufacture	80:107	industrial-scale manufacture of pre-crystallized acid whey powders	80:145	Short communication: Multi-component interactions causing solidification during industrial-scale manufacture of pre-crystallized acid whey powders.					
30292547	3	76	theme	%	627:627	arg1	concentrates					610:621	liquid AW concentrates	600:621	liquid AW concentrates (∼55% total solids)	600:641	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	3	76	theme	%	627:627	arg1	solids					635:640	∼55% total solids	624:640	∼55% total solids	624:640	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	2	77	theme	whey	366:369	arg1	proteins					371:378	whey proteins	366:378	whey proteins	366:378	Further processing of AW is often challenging due to its high mineral content, which can promote aggregation of whey proteins, which contributes to high viscosity of the liquid concentrate during subsequent lactose crystallization and drying steps.					
30292547	3	78	from	trials	727:732	arg1	microstructure					655:668	the microstructure	651:668	the microstructure of the final powders from 2 independent industrial-scale trials	651:732	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30292547	3	78	from	trials	727:732	arg1	powders					683:689	the final powders	673:689	the final powders from 2 independent industrial-scale trials	673:732	This study focuses on mineral precipitation, protein aggregation, and lactose crystallization in liquid AW concentrates (∼55% total solids), and on the microstructure of the final powders from 2 independent industrial-scale trials.					
30484006	12	0	theme	therapeutic	2095:2105	arg1	which					2069:2073	which	2069:2073	which	2069:2073	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	12	0	theme	therapeutic	2095:2105	arg1	target					2107:2112	a possible therapeutic target	2084:2112	a possible therapeutic target	2084:2112	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	11	1	theme	LPS-induced	1698:1708	arg1	ROS					1710:1712	LPS-induced ROS	1698:1712	LPS-induced ROS	1698:1712	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	7	2	theme	PDA	1149:1151	arg1	segments					1153:1160	PDA segments	1149:1160	PDA segments	1149:1160	Our results showed that the PDA extracts were mainly composed of dopamine, quinine, and PDA segments.					
30484006	10	3	theme	NF-κB	1576:1580	arg1	trans-locating					1582:1595	LPS-induced NF-κB trans-locating	1564:1595	LPS-induced NF-κB trans-locating	1564:1595	Similarly, PDA extracts effectively inhibited LPS-induced NF-κB trans-locating into nuclear by inactivation of the phosphorylation of IKK-α/β and IKβ-α.					
30484006	6	4	theme	molecular	1040:1048	arg1	mechanism					1050:1058	associated molecular mechanism	1029:1058	associated molecular mechanism	1029:1058	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	10	5	theme	PDA	1529:1531	arg1	extracts					1533:1540	PDA extracts	1529:1540	PDA extracts	1529:1540	Similarly, PDA extracts effectively inhibited LPS-induced NF-κB trans-locating into nuclear by inactivation of the phosphorylation of IKK-α/β and IKβ-α.					
30484006	6	6	theme	anti-inflammatory	916:932	arg1	ability					934:940	their anti-inflammatory ability	910:940	their anti-inflammatory ability	910:940	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	12	7	theme	potential	1887:1895	arg1	mechanism					1897:1905	a potential mechanism	1885:1905	a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target	1885:2112	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	12	8	theme	HO-1	2055:2058	arg1	pathway					2060:2066	simultaneous activation HO-1 pathway	2031:2066	simultaneous activation HO-1 pathway	2031:2066	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	6	9	theme	associated	1029:1038	arg1	mechanism					1050:1058	associated molecular mechanism	1029:1058	associated molecular mechanism	1029:1058	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	0	10	theme	Dependent	130:138	arg1	Pathways					150:157	Mediation TLR-4-MYD88 Dependent Signaling Pathways	108:157	Mediation TLR-4-MYD88 Dependent Signaling Pathways	108:157	Degradation Products of Polydopamine Restrained Inflammatory Response of LPS-Stimulated Macrophages Through Mediation TLR-4-MYD88 Dependent Signaling Pathways by Antioxidant.					
30484006	12	11	theme	macrophages	1971:1981	arg1	inflammation					1955:1966	inflammation	1955:1966	inflammation of macrophages	1955:1981	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	8	12	theme	extract	1370:1376	arg1	treatment					1378:1386	PDA extract treatment	1366:1386	PDA extract treatment	1366:1386	Furthermore, macrophages showed no cytotoxicity after PDA extract treatment with or without LPS, while the release levels of TNF-α and IL-6 by LPS-induced macrophages were decreased in dose-dependent by PDA extract treatment.					
30484006	12	13	theme	simultaneous	2031:2042	arg1	pathway					2060:2066	simultaneous activation HO-1 pathway	2031:2066	simultaneous activation HO-1 pathway	2031:2066	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	1	14	contain	has	194:196	arg1	PDA					189:191	PDA	189:191	PDA	189:191	Polydopamine (PDA) has a promising application as coating of biomaterials due to its favorable degradability and bioadaptability.					
30484006	1	14	contain	has	194:196	arg1	Polydopamine					175:186	Polydopamine	175:186	Polydopamine (PDA)	175:192	Polydopamine (PDA) has a promising application as coating of biomaterials due to its favorable degradability and bioadaptability.					
30484006	1	14	contain	has	194:196	arg2	application					210:220	a promising application	198:220	a promising application	198:220	Polydopamine (PDA) has a promising application as coating of biomaterials due to its favorable degradability and bioadaptability.					
30484006	11	15	theme	HO-1	1764:1767	arg1	protein					1792:1798	a critical protein	1781:1798	a critical protein of antioxidant signaling pathway	1781:1831	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	11	15	theme	HO-1	1764:1767	arg1	expression					1769:1778	HO-1 expression	1764:1778	HO-1 expression	1764:1778	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	8	16	theme	PDA	1366:1368	arg1	treatment					1378:1386	PDA extract treatment	1366:1386	PDA extract treatment	1366:1386	Furthermore, macrophages showed no cytotoxicity after PDA extract treatment with or without LPS, while the release levels of TNF-α and IL-6 by LPS-induced macrophages were decreased in dose-dependent by PDA extract treatment.					
30484006	6	17	theme	cytokines	1016:1024	arg1	levels					989:994	the expression levels	974:994	the expression levels of pro-inflammatory cytokines to associated molecular mechanism	974:1058	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	5	18	theme	HPLC	785:788	arg1	assay					790:794	HPLC assay	785:794	HPLC assay	785:794	Wherein, the composition of the degradation products was analyzed by HPLC assay.					
30484006	8	19	theme	LPS-induced	1306:1316	arg1	macrophages					1318:1328	LPS-induced macrophages	1306:1328	LPS-induced macrophages	1306:1328	Furthermore, macrophages showed no cytotoxicity after PDA extract treatment with or without LPS, while the release levels of TNF-α and IL-6 by LPS-induced macrophages were decreased in dose-dependent by PDA extract treatment.					
30484006	0	20	theme	Signaling	140:148	arg1	Pathways					150:157	Mediation TLR-4-MYD88 Dependent Signaling Pathways	108:157	Mediation TLR-4-MYD88 Dependent Signaling Pathways	108:157	Degradation Products of Polydopamine Restrained Inflammatory Response of LPS-Stimulated Macrophages Through Mediation TLR-4-MYD88 Dependent Signaling Pathways by Antioxidant.					
30484006	11	21	theme	ROS	1710:1712	arg1	production					1684:1693	the production	1680:1693	the production of LPS-induced ROS	1680:1712	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	0	22	theme	Inflammatory	48:59	arg1	Response					61:68	Inflammatory Response	48:68	Inflammatory Response of LPS-Stimulated Macrophages	48:98	Degradation Products of Polydopamine Restrained Inflammatory Response of LPS-Stimulated Macrophages Through Mediation TLR-4-MYD88 Dependent Signaling Pathways by Antioxidant.					
30484006	0	23	theme	Degradation	0:10	arg1	Products					12:19	Degradation Products	0:19	Degradation Products of Polydopamine	0:35	Degradation Products of Polydopamine Restrained Inflammatory Response of LPS-Stimulated Macrophages Through Mediation TLR-4-MYD88 Dependent Signaling Pathways by Antioxidant.					
30484006	11	24	theme	pathway	1825:1831	arg1	protein					1792:1798	a critical protein	1781:1798	a critical protein of antioxidant signaling pathway	1781:1831	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	11	24	theme	pathway	1825:1831	arg1	expression					1769:1778	HO-1 expression	1764:1778	HO-1 expression	1764:1778	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	1	25	theme	promising	200:208	arg1	application					210:220	a promising application	198:220	a promising application	198:220	Polydopamine (PDA) has a promising application as coating of biomaterials due to its favorable degradability and bioadaptability.					
30484006	8	26	theme	extract	1221:1227	arg1	treatment					1229:1237	PDA extract treatment	1217:1237	PDA extract treatment with or without LPS	1217:1257	Furthermore, macrophages showed no cytotoxicity after PDA extract treatment with or without LPS, while the release levels of TNF-α and IL-6 by LPS-induced macrophages were decreased in dose-dependent by PDA extract treatment.					
30484006	12	27	theme	possible	2086:2093	arg1	which					2069:2073	which	2069:2073	which	2069:2073	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	12	27	theme	possible	2086:2093	arg1	target					2107:2112	a possible therapeutic target	2084:2112	a possible therapeutic target	2084:2112	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	12	28	theme	PDA	1940:1942	arg1	products					1928:1935	degradation products	1916:1935	degradation products of PDA	1916:1942	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	10	29	theme	LPS-induced	1564:1574	arg1	trans-locating					1582:1595	LPS-induced NF-κB trans-locating	1564:1595	LPS-induced NF-κB trans-locating	1564:1595	Similarly, PDA extracts effectively inhibited LPS-induced NF-κB trans-locating into nuclear by inactivation of the phosphorylation of IKK-α/β and IKβ-α.					
30484006	9	30	theme	PDA	1477:1479	arg1	extracts					1481:1488	PDA extracts	1477:1488	PDA extracts	1477:1488	Additionally, TLR-4 and MYD88 expression in protein and RNA level were downregulated by PDA extracts in LPS-induced macrophages.					
30484006	3	31	theme	degradable	423:432	arg1	products					434:441	degradable products	423:441	degradable products of PDA	423:448	Herein, we investigated whether degradable products of PDA could affect inflammatory response in lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages.					
30484006	6	32	theme	pro-inflammatory	999:1014	arg1	cytokines					1016:1024	pro-inflammatory cytokines	999:1024	pro-inflammatory cytokines to associated molecular mechanism	999:1058	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	10	33	theme	IKβ-α	1664:1668	arg1	phosphorylation					1633:1647	the phosphorylation	1629:1647	the phosphorylation of IKK-α/β and IKβ-α	1629:1668	Similarly, PDA extracts effectively inhibited LPS-induced NF-κB trans-locating into nuclear by inactivation of the phosphorylation of IKK-α/β and IKβ-α.					
30484006	2	34	theme	anti-inflammatory	339:355	arg1	capacity					357:364	anti-inflammatory capacity	339:364	anti-inflammatory capacity	339:364	However, its bioactivity, such as anti-inflammatory capacity, was still little known.					
30484006	3	35	theme	inflammatory	463:474	arg1	response					476:483	inflammatory response	463:483	inflammatory response in lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	463:554	Herein, we investigated whether degradable products of PDA could affect inflammatory response in lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages.					
30484006	0	36	theme	Polydopamine	24:35	arg1	Products					12:19	Degradation Products	0:19	Degradation Products of Polydopamine	0:35	Degradation Products of Polydopamine Restrained Inflammatory Response of LPS-Stimulated Macrophages Through Mediation TLR-4-MYD88 Dependent Signaling Pathways by Antioxidant.					
30484006	11	37	theme	signaling	1815:1823	arg1	pathway					1825:1831	antioxidant signaling pathway	1803:1831	antioxidant signaling pathway	1803:1831	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	6	38	from	levels	989:994	arg1	macrophages					957:967	LPS-induced macrophages	945:967	LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism	945:1058	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	11	39	theme	antioxidant	1803:1813	arg1	pathway					1825:1831	antioxidant signaling pathway	1803:1831	antioxidant signaling pathway	1803:1831	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	2	40	dep	little	377:382	arg1	known					384:388	known	384:388	known	384:388	However, its bioactivity, such as anti-inflammatory capacity, was still little known.					
30484006	4	41	theme	cell	684:687	arg1	medium					697:702	cell culture medium	684:702	cell culture medium for 3 days	684:713	The supernatants containing degradation products of PDA, annotated as PDA extracts, were collected after PDA being immersed in cell culture medium for 3 days.					
30484006	12	42	theme	activation	2044:2053	arg1	pathway					2060:2066	simultaneous activation HO-1 pathway	2031:2066	simultaneous activation HO-1 pathway	2031:2066	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	4	43	theme	PDA	627:629	arg1	extracts					631:638	PDA extracts	627:638	PDA extracts	627:638	The supernatants containing degradation products of PDA, annotated as PDA extracts, were collected after PDA being immersed in cell culture medium for 3 days.					
30484006	5	44	theme	degradation	748:758	arg1	products					760:767	the degradation products	744:767	the degradation products	744:767	Wherein, the composition of the degradation products was analyzed by HPLC assay.					
30484006	12	45	theme	downregulation	1987:2000	arg1	pathway					2019:2025	downregulation TLR-4-MYD88-NFκB pathway	1987:2025	downregulation TLR-4-MYD88-NFκB pathway	1987:2025	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	9	46	theme	LPS-induced	1493:1503	arg1	macrophages					1505:1515	LPS-induced macrophages	1493:1515	LPS-induced macrophages	1493:1515	Additionally, TLR-4 and MYD88 expression in protein and RNA level were downregulated by PDA extracts in LPS-induced macrophages.					
30484006	3	47	theme	PDA	446:448	arg1	products					434:441	degradable products	423:441	degradable products of PDA	423:448	Herein, we investigated whether degradable products of PDA could affect inflammatory response in lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages.					
30484006	6	48	theme	expression	978:987	arg1	levels					989:994	the expression levels	974:994	the expression levels of pro-inflammatory cytokines to associated molecular mechanism	974:1058	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	5	49	theme	products	760:767	arg1	composition					729:739	the composition	725:739	the composition of the degradation products	725:767	Wherein, the composition of the degradation products was analyzed by HPLC assay.					
30484006	5	49	theme	products	760:767	arg1	Wherein					716:722	Wherein	716:722	Wherein	716:722	Wherein, the composition of the degradation products was analyzed by HPLC assay.					
30484006	6	50	theme	concentration	869:881	arg1	%					855:855	25%	853:855	25% of original concentration	853:881	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	6	50	theme	concentration	869:881	arg1	concentration					869:881	original concentration	860:881	original concentration	860:881	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	6	50	theme	concentration	869:881	arg1	%					841:841	100%	838:841	100%	838:841	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	6	50	theme	concentration	869:881	arg1	%					846:846	50%	844:846	50%	844:846	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	8	51	theme	release	1270:1276	arg1	levels					1278:1283	the release levels	1266:1283	the release levels of TNF-α and IL-6 by LPS-induced macrophages	1266:1328	Furthermore, macrophages showed no cytotoxicity after PDA extract treatment with or without LPS, while the release levels of TNF-α and IL-6 by LPS-induced macrophages were decreased in dose-dependent by PDA extract treatment.					
30484006	1	52	theme	biomaterials	236:247	arg1	coating					225:231	coating	225:231	coating of biomaterials due to its favorable degradability and bioadaptability	225:302	Polydopamine (PDA) has a promising application as coating of biomaterials due to its favorable degradability and bioadaptability.					
30484006	9	53	theme	RNA	1445:1447	arg1	level					1449:1453	RNA level	1445:1453	RNA level	1445:1453	Additionally, TLR-4 and MYD88 expression in protein and RNA level were downregulated by PDA extracts in LPS-induced macrophages.					
30484006	6	54	theme	original	860:867	arg1	concentration					869:881	original concentration	860:881	original concentration	860:881	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	10	55	theme	IKK-α/β	1652:1658	arg1	phosphorylation					1633:1647	the phosphorylation	1629:1647	the phosphorylation of IKK-α/β and IKβ-α	1629:1668	Similarly, PDA extracts effectively inhibited LPS-induced NF-κB trans-locating into nuclear by inactivation of the phosphorylation of IKK-α/β and IKβ-α.					
30484006	4	56	theme	culture	689:695	arg1	medium					697:702	cell culture medium	684:702	cell culture medium for 3 days	684:713	The supernatants containing degradation products of PDA, annotated as PDA extracts, were collected after PDA being immersed in cell culture medium for 3 days.					
30484006	4	57	theme	degradation	585:595	arg1	products					597:604	degradation products	585:604	degradation products of PDA, annotated as PDA extracts,	585:639	The supernatants containing degradation products of PDA, annotated as PDA extracts, were collected after PDA being immersed in cell culture medium for 3 days.					
30484006	10	58	theme	phosphorylation	1633:1647	arg1	inactivation					1613:1624	inactivation	1613:1624	inactivation of the phosphorylation of IKK-α/β and IKβ-α	1613:1668	Similarly, PDA extracts effectively inhibited LPS-induced NF-κB trans-locating into nuclear by inactivation of the phosphorylation of IKK-α/β and IKβ-α.					
30484006	6	59	theme	PDA	807:809	arg1	extracts					811:818	Collected PDA extracts	797:818	Collected PDA extracts	797:818	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	3	60	theme	THP-1-derived	530:542	arg1	macrophages					544:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	Herein, we investigated whether degradable products of PDA could affect inflammatory response in lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages.					
30484006	12	61	theme	TLR-4-MYD88-NFκB	2002:2017	arg1	pathway					2019:2025	downregulation TLR-4-MYD88-NFκB pathway	1987:2025	downregulation TLR-4-MYD88-NFκB pathway	1987:2025	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	4	62	theme	PDA	609:611	arg1	products					597:604	degradation products	585:604	degradation products of PDA, annotated as PDA extracts,	585:639	The supernatants containing degradation products of PDA, annotated as PDA extracts, were collected after PDA being immersed in cell culture medium for 3 days.					
30484006	7	63	theme	PDA	1089:1091	arg1	extracts					1093:1100	the PDA extracts	1085:1100	the PDA extracts	1085:1100	Our results showed that the PDA extracts were mainly composed of dopamine, quinine, and PDA segments.					
30484006	9	64	from	TLR-4	1403:1407	arg1	protein					1433:1439	protein	1433:1439	protein	1433:1439	Additionally, TLR-4 and MYD88 expression in protein and RNA level were downregulated by PDA extracts in LPS-induced macrophages.					
30484006	9	64	from	TLR-4	1403:1407	arg1	level					1449:1453	RNA level	1445:1453	RNA level	1445:1453	Additionally, TLR-4 and MYD88 expression in protein and RNA level were downregulated by PDA extracts in LPS-induced macrophages.					
30484006	12	65	theme	degradation	1916:1926	arg1	products					1928:1935	degradation products	1916:1935	degradation products of PDA	1916:1942	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	6	66	theme	Collected	797:805	arg1	extracts					811:818	Collected PDA extracts	797:818	Collected PDA extracts	797:818	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	2	67	dep	such	331:334	arg1	as					336:337	as	336:337	as	336:337	However, its bioactivity, such as anti-inflammatory capacity, was still little known.					
30484006	6	68	theme	LPS-induced	945:955	arg1	macrophages					957:967	LPS-induced macrophages	945:967	LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism	945:1058	Collected PDA extracts were diluted into 100%, 50%, and 25% of original concentration, respectively, to evaluate their anti-inflammatory ability on LPS-induced macrophages from the expression levels of pro-inflammatory cytokines to associated molecular mechanism.					
30484006	3	69	link	THP-1-derived	530:542	arg1	macrophages					544:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	Herein, we investigated whether degradable products of PDA could affect inflammatory response in lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages.					
30484006	3	70	theme	-stimulated	512:522	arg1	macrophages					544:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	Herein, we investigated whether degradable products of PDA could affect inflammatory response in lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages.					
30484006	9	71	theme	MYD88	1413:1417	arg1	expression					1419:1428	MYD88 expression	1413:1428	MYD88 expression in protein and RNA level	1413:1453	Additionally, TLR-4 and MYD88 expression in protein and RNA level were downregulated by PDA extracts in LPS-induced macrophages.					
30484006	9	72	from	expression	1419:1428	arg1	protein					1433:1439	protein	1433:1439	protein	1433:1439	Additionally, TLR-4 and MYD88 expression in protein and RNA level were downregulated by PDA extracts in LPS-induced macrophages.					
30484006	9	72	from	expression	1419:1428	arg1	level					1449:1453	RNA level	1445:1453	RNA level	1445:1453	Additionally, TLR-4 and MYD88 expression in protein and RNA level were downregulated by PDA extracts in LPS-induced macrophages.					
30484006	8	73	theme	PDA	1217:1219	arg1	treatment					1229:1237	PDA extract treatment	1217:1237	PDA extract treatment with or without LPS	1217:1257	Furthermore, macrophages showed no cytotoxicity after PDA extract treatment with or without LPS, while the release levels of TNF-α and IL-6 by LPS-induced macrophages were decreased in dose-dependent by PDA extract treatment.					
30484006	3	74	theme	human	524:528	arg1	macrophages					544:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	Herein, we investigated whether degradable products of PDA could affect inflammatory response in lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages.					
30484006	0	75	theme	Macrophages	88:98	arg1	Response					61:68	Inflammatory Response	48:68	Inflammatory Response of LPS-Stimulated Macrophages	48:98	Degradation Products of Polydopamine Restrained Inflammatory Response of LPS-Stimulated Macrophages Through Mediation TLR-4-MYD88 Dependent Signaling Pathways by Antioxidant.					
30484006	11	76	theme	critical	1783:1790	arg1	protein					1792:1798	a critical protein	1781:1798	a critical protein of antioxidant signaling pathway	1781:1831	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	11	76	theme	critical	1783:1790	arg1	expression					1769:1778	HO-1 expression	1764:1778	HO-1 expression	1764:1778	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	11	77	theme	PDA	1729:1731	arg1	extracts					1733:1740	PDA extracts	1729:1740	PDA extracts	1729:1740	Of note, the production of LPS-induced ROS was reduced by PDA extracts in macrophages, while HO-1 expression, a critical protein of antioxidant signaling pathway, was increased.					
30484006	8	78	theme	TNF-α	1288:1292	arg1	levels					1278:1283	the release levels	1266:1283	the release levels of TNF-α and IL-6 by LPS-induced macrophages	1266:1328	Furthermore, macrophages showed no cytotoxicity after PDA extract treatment with or without LPS, while the release levels of TNF-α and IL-6 by LPS-induced macrophages were decreased in dose-dependent by PDA extract treatment.					
30484006	1	79	theme	favorable	260:268	arg1	degradability					270:282	its favorable degradability	256:282	its favorable degradability	256:282	Polydopamine (PDA) has a promising application as coating of biomaterials due to its favorable degradability and bioadaptability.					
30484006	0	80	theme	LPS-Stimulated	73:86	arg1	Macrophages					88:98	LPS-Stimulated Macrophages	73:98	LPS-Stimulated Macrophages	73:98	Degradation Products of Polydopamine Restrained Inflammatory Response of LPS-Stimulated Macrophages Through Mediation TLR-4-MYD88 Dependent Signaling Pathways by Antioxidant.					
30484006	8	81	theme	IL-6	1298:1301	arg1	levels					1278:1283	the release levels	1266:1283	the release levels of TNF-α and IL-6 by LPS-induced macrophages	1266:1328	Furthermore, macrophages showed no cytotoxicity after PDA extract treatment with or without LPS, while the release levels of TNF-α and IL-6 by LPS-induced macrophages were decreased in dose-dependent by PDA extract treatment.					
30484006	3	82	from	response	476:483	arg1	macrophages					544:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages	488:554	Herein, we investigated whether degradable products of PDA could affect inflammatory response in lipopolysaccharide (LPS)-stimulated human THP-1-derived macrophages.					
30484006	4	83	contain	containing	574:583	arg1	supernatants					561:572	The supernatants	557:572	The supernatants containing degradation products of PDA, annotated as PDA extracts,	557:639	The supernatants containing degradation products of PDA, annotated as PDA extracts, were collected after PDA being immersed in cell culture medium for 3 days.					
30484006	4	83	contain	containing	574:583	arg2	products					597:604	degradation products	585:604	degradation products of PDA, annotated as PDA extracts,	585:639	The supernatants containing degradation products of PDA, annotated as PDA extracts, were collected after PDA being immersed in cell culture medium for 3 days.					
30484006	12	84	dep	suppressed	1944:1953	arg1	which					2069:2073	which	2069:2073	which	2069:2073	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
30484006	12	84	dep	suppressed	1944:1953	arg1	target					2107:2112	a possible therapeutic target	2084:2112	a possible therapeutic target	2084:2112	Based on these results, we proposed a potential mechanism by which degradation products of PDA suppressed inflammation of macrophages via downregulation TLR-4-MYD88-NFκB pathway and simultaneous activation HO-1 pathway, which might be a possible therapeutic target.					
31155318	13	0	theme	diagnostic	2226:2235	arg1	sensitivity					2237:2247	superior diagnostic sensitivity	2217:2247	superior diagnostic sensitivity	2217:2247	INTERPRETATION In comparison to AlereLAM, FujiLAM offers superior diagnostic sensitivity, while maintaining specificity, and could transform rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV.					
31155318	11	1	theme	estimated	1736:1744	arg1	%					1777:1777	90·8%	1773:1777	90·8% (86·0 to 94·4)	1773:1792	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	11	1	theme	estimated	1736:1744	arg1	specificity					1746:1756	the estimated specificity	1732:1756	the estimated specificity of FujiLAM	1732:1767	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	12	2	dep	%	2074:2074	arg1	to					2082:2083	to	2082:2083	to	2082:2083	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	12	2	dep	%	2074:2074	arg1	%					2156:2156	difference 26·7%	2141:2156	difference 26·7%	2141:2156	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	2	3	theme	TB	307:308	arg1	[AlereLAM					317:325	TB LAM Ag [AlereLAM	307:325	TB LAM Ag [AlereLAM	307:325	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	9	4	theme	urine	1365:1369	arg1	samples					1371:1377	urine samples	1365:1377	urine samples from 968 hospital inpatients with HIV	1365:1415	FINDINGS Between April 18, 2018, and May 3, 2018, urine samples from 968 hospital inpatients with HIV were evaluated.					
31155318	1	5	theme	Most	117:120	arg1	deaths					143:148	BACKGROUND Most tuberculosis-related deaths	106:148	BACKGROUND Most tuberculosis-related deaths in people with HIV	106:167	BACKGROUND Most tuberculosis-related deaths in people with HIV could be prevented with earlier diagnosis and treatment.					
31155318	5	6	dep	Bank	781:784	arg1	FIND					767:770	FIND	767:770	FIND	767:770	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	5	6	dep	Bank	781:784	arg1	the					763:765	the	763:765	the	763:765	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	11	7	dep	53·0	1647:1650	arg1	to					1652:1653	to	1652:1653	to	1652:1653	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	11	8	theme	FujiLAM	1761:1767	arg1	%					1777:1777	90·8%	1773:1777	90·8% (86·0 to 94·4)	1773:1792	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	11	8	theme	FujiLAM	1761:1767	arg1	specificity					1746:1756	the estimated specificity	1732:1756	the estimated specificity of FujiLAM	1732:1767	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	8	9	theme	FujiLAM	1193:1199	arg1	accuracy					1181:1188	the diagnostic accuracy	1166:1188	the diagnostic accuracy of FujiLAM	1166:1199	The primary objective was to assess the diagnostic accuracy of FujiLAM compared with AlereLAM, against microbiological and composite reference standards (including clinical diagnoses).					
31155318	10	10	theme	median	1508:1513	arg1	count					1519:1523	the median CD4 count	1504:1523	the median CD4 count	1504:1523	The prevalence of microbiologically-confirmed tuberculosis was 62% and the median CD4 count was 86 cells per μL.					
31155318	10	10	theme	median	1508:1513	arg1	cells					1532:1536	86 cells	1529:1536	86 cells	1529:1536	The prevalence of microbiologically-confirmed tuberculosis was 62% and the median CD4 count was 86 cells per μL.					
31155318	11	11	theme	estimated	1596:1604	arg1	%					1637:1637	70·4%	1633:1637	70·4%	1633:1637	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	11	11	theme	estimated	1596:1604	arg1	sensitivity					1606:1616	the estimated sensitivity	1592:1616	the estimated sensitivity of FujiLAM	1592:1627	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	15	12	theme	Global	2477:2482	arg1	Fund					2513:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	12	theme	Global	2477:2482	arg1	Department					2522:2531	Department	2522:2531	Department	2522:2531	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	12	theme	Global	2477:2482	arg1	Bill					2599:2602	Bill	2599:2602	Bill	2599:2602	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	12	theme	Global	2477:2482	arg1	Ministry					2570:2577	Ministry	2570:2577	Ministry	2570:2577	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	13	theme	Innovative	2491:2500	arg1	Fund					2513:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	13	theme	Innovative	2491:2500	arg1	Department					2522:2531	Department	2522:2531	Department	2522:2531	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	13	theme	Innovative	2491:2500	arg1	Bill					2599:2602	Bill	2599:2602	Bill	2599:2602	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	13	theme	Innovative	2491:2500	arg1	Ministry					2570:2577	Ministry	2570:2577	Ministry	2570:2577	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	9	14	theme	hospital	1388:1395	arg1	inpatients					1397:1406	968 hospital inpatients	1384:1406	968 hospital inpatients with HIV	1384:1415	FINDINGS Between April 18, 2018, and May 3, 2018, urine samples from 968 hospital inpatients with HIV were evaluated.					
31155318	13	15	theme	superior	2217:2224	arg1	sensitivity					2237:2247	superior diagnostic sensitivity	2217:2247	superior diagnostic sensitivity	2217:2247	INTERPRETATION In comparison to AlereLAM, FujiLAM offers superior diagnostic sensitivity, while maintaining specificity, and could transform rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV.					
31155318	12	16	theme	assays	1915:1920	arg1	higher					1926:1931	higher	1926:1931	higher	1926:1931	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	12	16	theme	assays	1915:1920	arg1	specificity					1895:1905	the specificity	1891:1905	the specificity of both assays	1891:1920	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	5	17	theme	accuracy	697:704	arg1	study					706:710	this diagnostic accuracy study	681:710	this diagnostic accuracy study	681:710	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	2	18	theme	Ag	314:315	arg1	[AlereLAM					317:325	TB LAM Ag [AlereLAM	307:325	TB LAM Ag [AlereLAM	307:325	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	15	19	theme	Africa	2830:2835	arg1	Department					2751:2760	Department	2751:2760	Department of Science	2751:2771	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	19	theme	Africa	2830:2835	arg1	Foundation					2810:2819	National Research Foundation	2792:2819	National Research Foundation	2792:2819	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	19	theme	Africa	2830:2835	arg1	Trust					2744:2748	Foreign Affairs and Trade, Wellcome Trust	2708:2748	Foreign Affairs and Trade, Wellcome Trust	2708:2748	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	19	theme	Africa	2830:2835	arg1	Technology					2777:2786	Technology	2777:2786	Technology	2777:2786	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	8	20	theme	microbiological	1233:1247	arg1	standards					1273:1281	microbiological and composite reference standards	1233:1281	microbiological and composite reference standards (including clinical diagnoses)	1233:1312	The primary objective was to assess the diagnostic accuracy of FujiLAM compared with AlereLAM, against microbiological and composite reference standards (including clinical diagnoses).					
31155318	0	21	theme	tuberculosis	38:49	arg1	test					51:54	Novel lipoarabinomannan point-of-care tuberculosis test	0:54	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.	0:104	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.					
31155318	8	22	theme	composite	1253:1261	arg1	standards					1273:1281	microbiological and composite reference standards	1233:1281	microbiological and composite reference standards (including clinical diagnoses)	1233:1312	The primary objective was to assess the diagnostic accuracy of FujiLAM compared with AlereLAM, against microbiological and composite reference standards (including clinical diagnoses).					
31155318	15	23	dep	Fund	2513:2516	arg1	Gates					2614:2618	Gates	2614:2618	Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council	2614:2879	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	12	24	dep	%	2110:2110	arg1	to					2118:2119	to	2118:2119	to	2118:2119	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	1	25	theme	tuberculosis-related	122:141	arg1	deaths					143:148	BACKGROUND Most tuberculosis-related deaths	106:148	BACKGROUND Most tuberculosis-related deaths in people with HIV	106:167	BACKGROUND Most tuberculosis-related deaths in people with HIV could be prevented with earlier diagnosis and treatment.					
31155318	15	26	theme	Research	2801:2808	arg1	Foundation					2810:2819	National Research Foundation	2792:2819	National Research Foundation	2792:2819	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	11	27	theme	difference	1830:1839	arg1	AlereLAM					1820:1827	AlereLAM	1820:1827	AlereLAM (difference -4·2%)	1820:1846	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	11	27	theme	difference	1830:1839	arg1	%					1845:1845	difference -4·2%	1830:1845	difference -4·2%	1830:1845	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	6	28	theme	Urine	992:996	arg1	samples					998:1004	Urine samples	992:1004	Urine samples	992:1004	Urine samples were tested using FujiLAM and AlereLAM assays.					
31155318	14	29	theme	prospective	2445:2455	arg1	assessment					2457:2466	prospective assessment	2445:2466	prospective assessment	2445:2466	The applicability of FujiLAM for settings of intended use requires prospective assessment.					
31155318	12	30	theme	composite	1861:1869	arg1	reference					1871:1879	the composite reference	1857:1879	the composite reference standard	1857:1888	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	2	31	contain	has	329:331	arg1	test					285:288	The only commercially available tuberculosis point-of-care test	226:288	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM])	226:327	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	2	31	contain	has	329:331	arg2	sensitivity					344:354	suboptimal sensitivity	333:354	suboptimal sensitivity	333:354	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	5	32	theme	Cape	808:811	arg1	Town					813:816	Cape Town	808:816	Cape Town	808:816	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	13	33	theme	rapid	2301:2305	arg1	diagnosis					2334:2342	rapid point-of-care tuberculosis diagnosis	2301:2342	rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV	2301:2375	INTERPRETATION In comparison to AlereLAM, FujiLAM offers superior diagnostic sensitivity, while maintaining specificity, and could transform rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV.					
31155318	12	34	theme	difference	2141:2150	arg1	%					2156:2156	difference 26·7%	2141:2156	difference 26·7%	2141:2156	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	12	35	theme	standard	1881:1888	arg1	reference					1871:1879	the composite reference	1857:1879	the composite reference standard	1857:1888	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	11	36	dep	51·8	1690:1693	arg1	to					1687:1688	to	1687:1688	to	1687:1688	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	5	37	theme	aged	878:881	arg1	years					887:891	aged ≥18 years	878:891	aged ≥18 years	878:891	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	5	37	theme	aged	878:881	arg1	inpatients					866:875	hospital inpatients	857:875	hospital inpatients (aged ≥18 years) with HIV	857:901	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	15	38	theme	Research	2864:2871	arg1	Council					2873:2879	African Medical Research Council	2848:2879	African Medical Research Council	2848:2879	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	1	39	theme	BACKGROUND	106:115	arg1	deaths					143:148	BACKGROUND Most tuberculosis-related deaths	106:148	BACKGROUND Most tuberculosis-related deaths in people with HIV	106:167	BACKGROUND Most tuberculosis-related deaths in people with HIV could be prevented with earlier diagnosis and treatment.					
31155318	0	40	theme	accuracy	90:97	arg1	study					99:103	a diagnostic accuracy study	77:103	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.	0:104	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.					
31155318	5	41	theme	cohort	940:945	arg1	studies					947:953	three independent prospective cohort studies	910:953	three independent prospective cohort studies done at two South African hospitals	910:989	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	15	42	theme	African	2848:2854	arg1	Council					2873:2879	African Medical Research Council	2848:2879	African Medical Research Council	2848:2879	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	12	43	theme	difference	2005:2014	arg1	%					2020:2020	difference -2·5%	2005:2020	difference -2·5%	2005:2020	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	5	44	theme	urine	735:739	arg1	samples					741:747	biobanked urine samples	725:747	biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals	725:989	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	1	45	from	deaths	143:148	arg1	people					153:158	people	153:158	people with HIV	153:167	BACKGROUND Most tuberculosis-related deaths in people with HIV could be prevented with earlier diagnosis and treatment.					
31155318	15	46	theme	Wellcome	2735:2742	arg1	Trust					2744:2748	Foreign Affairs and Trade, Wellcome Trust	2708:2748	Foreign Affairs and Trade, Wellcome Trust	2708:2748	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	3	47	theme	TB	430:431	arg1	assay					447:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay has been developed to improve the sensitivity of AlereLAM.					
31155318	0	48	theme	Novel	0:4	arg1	test					51:54	Novel lipoarabinomannan point-of-care tuberculosis test	0:54	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.	0:104	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.					
31155318	15	49	dep	Foundation	2620:2629	arg1	Council					2873:2879	African Medical Research Council	2848:2879	African Medical Research Council	2848:2879	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	0	50	theme	point-of-care	24:36	arg1	test					51:54	Novel lipoarabinomannan point-of-care tuberculosis test	0:54	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.	0:104	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.					
31155318	5	51	theme	independent	916:926	arg1	studies					947:953	three independent prospective cohort studies	910:953	three independent prospective cohort studies done at two South African hospitals	910:989	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	15	52	theme	Affairs	2716:2722	arg1	Trust					2744:2748	Foreign Affairs and Trade, Wellcome Trust	2708:2748	Foreign Affairs and Trade, Wellcome Trust	2708:2748	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	3	53	theme	Fujifilm	413:420	arg1	assay					447:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay has been developed to improve the sensitivity of AlereLAM.					
31155318	9	54	with	inpatients	1397:1406	arg1	HIV					1413:1415	HIV	1413:1415	HIV	1413:1415	FINDINGS Between April 18, 2018, and May 3, 2018, urine samples from 968 hospital inpatients with HIV were evaluated.					
31155318	7	55	theme	other	1110:1114	arg1	results					1121:1127	other test results	1110:1127	other test results	1110:1127	The conduct and reporting of each test was done blind to other test results.					
31155318	5	56	theme	Specimen	772:779	arg1	Bank					781:784	FIND Specimen Bank	767:784	FIND Specimen Bank	767:784	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	14	57	theme	intended	2423:2430	arg1	use					2432:2434	intended use	2423:2434	intended use	2423:2434	The applicability of FujiLAM for settings of intended use requires prospective assessment.					
31155318	0	58	dep	test	51:54	arg1	study					99:103	a diagnostic accuracy study	77:103	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.	0:104	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.					
31155318	9	59	from	inpatients	1397:1406	arg1	samples					1371:1377	urine samples	1365:1377	urine samples from 968 hospital inpatients with HIV	1365:1415	FINDINGS Between April 18, 2018, and May 3, 2018, urine samples from 968 hospital inpatients with HIV were evaluated.					
31155318	2	60	theme	tuberculosis	258:269	arg1	test					285:288	The only commercially available tuberculosis point-of-care test	226:288	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM])	226:327	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	10	61	theme	microbiologically-confirmed	1451:1477	arg1	tuberculosis					1479:1490	microbiologically-confirmed tuberculosis	1451:1490	microbiologically-confirmed tuberculosis	1451:1490	The prevalence of microbiologically-confirmed tuberculosis was 62% and the median CD4 count was 86 cells per μL.					
31155318	2	62	theme	suboptimal	333:342	arg1	sensitivity					344:354	suboptimal sensitivity	333:354	suboptimal sensitivity	333:354	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	12	63	dep	lower	2063:2067	arg1	%					2074:2074	64·9% [50·1 to 76·7] for FujiLAM	2070:2101	64·9% [50·1 to 76·7] for FujiLAM	2070:2101	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	12	63	dep	lower	2063:2067	arg1	%					2110:2110	38·2% [28·1 to 47·3] for AlereLAM	2106:2138	38·2% [28·1 to 47·3] for AlereLAM	2106:2138	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	12	64	dep	%	1938:1938	arg1	to					1946:1947	to	1946:1947	to	1946:1947	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	12	64	dep	%	1938:1938	arg1	%					2020:2020	difference -2·5%	2005:2020	difference -2·5%	2005:2020	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	14	65	theme	FujiLAM	2399:2405	arg1	applicability					2382:2394	The applicability	2378:2394	The applicability of FujiLAM for settings of intended use	2378:2434	The applicability of FujiLAM for settings of intended use requires prospective assessment.					
31155318	11	66	theme	%	1642:1642	arg1	53·0					1647:1650	95% CI 53·0 to 83·1	1640:1658	95% CI 53·0 to 83·1	1640:1658	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	0	67	with	people	60:65	arg1	HIV					72:74	HIV	72:74	HIV	72:74	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.					
31155318	3	68	theme	AlereLAM	502:509	arg1	sensitivity					487:497	the sensitivity	483:497	the sensitivity of AlereLAM	483:509	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay has been developed to improve the sensitivity of AlereLAM.					
31155318	11	69	dep	%	1637:1637	arg1	53·0					1647:1650	95% CI 53·0 to 83·1	1640:1658	95% CI 53·0 to 83·1	1640:1658	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	5	70	theme	African	973:979	arg1	hospitals					981:989	two South African hospitals	963:989	two South African hospitals	963:989	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	12	71	theme	38·2	2106:2109	arg1	%					2110:2110	38·2% [28·1 to 47·3] for AlereLAM	2106:2138	38·2% [28·1 to 47·3] for AlereLAM	2106:2138	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	7	72	theme	test	1087:1090	arg1	reporting					1069:1077	reporting	1069:1077	reporting	1069:1077	The conduct and reporting of each test was done blind to other test results.					
31155318	7	72	theme	test	1087:1090	arg1	conduct					1057:1063	conduct	1057:1063	conduct	1057:1063	The conduct and reporting of each test was done blind to other test results.					
31155318	13	73	with	inpatients	2357:2366	arg1	HIV					2373:2375	HIV	2373:2375	HIV	2373:2375	INTERPRETATION In comparison to AlereLAM, FujiLAM offers superior diagnostic sensitivity, while maintaining specificity, and could transform rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV.					
31155318	4	74	theme	assay	563:567	arg1	accuracy					539:546	the diagnostic accuracy	524:546	the diagnostic accuracy of the FujiLAM assay for the detection of tuberculosis	524:601	We assessed the diagnostic accuracy of the FujiLAM assay for the detection of tuberculosis in hospital inpatients with HIV compared with the AlereLAM assay.					
31155318	11	75	theme	standard	1582:1589	arg1	reference					1572:1580	the microbiological reference	1552:1580	the microbiological reference standard	1552:1589	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	2	76	theme	LAM	310:312	arg1	[AlereLAM					317:325	TB LAM Ag [AlereLAM	307:325	TB LAM Ag [AlereLAM	307:325	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	10	77	theme	CD4	1515:1517	arg1	count					1519:1523	the median CD4 count	1504:1523	the median CD4 count	1504:1523	The prevalence of microbiologically-confirmed tuberculosis was 62% and the median CD4 count was 86 cells per μL.					
31155318	10	77	theme	CD4	1515:1517	arg1	cells					1532:1536	86 cells	1529:1536	86 cells	1529:1536	The prevalence of microbiologically-confirmed tuberculosis was 62% and the median CD4 count was 86 cells per μL.					
31155318	11	78	theme	FujiLAM	1621:1627	arg1	%					1637:1637	70·4%	1633:1637	70·4%	1633:1637	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	11	78	theme	FujiLAM	1621:1627	arg1	sensitivity					1606:1616	the estimated sensitivity	1592:1616	the estimated sensitivity of FujiLAM	1592:1627	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	15	79	theme	Technology	2502:2511	arg1	Fund					2513:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	79	theme	Technology	2502:2511	arg1	Department					2522:2531	Department	2522:2531	Department	2522:2531	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	79	theme	Technology	2502:2511	arg1	Bill					2599:2602	Bill	2599:2602	Bill	2599:2602	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	79	theme	Technology	2502:2511	arg1	Ministry					2570:2577	Ministry	2570:2577	Ministry	2570:2577	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	80	theme	Health	2484:2489	arg1	Fund					2513:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	80	theme	Health	2484:2489	arg1	Department					2522:2531	Department	2522:2531	Department	2522:2531	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	80	theme	Health	2484:2489	arg1	Bill					2599:2602	Bill	2599:2602	Bill	2599:2602	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	80	theme	Health	2484:2489	arg1	Ministry					2570:2577	Ministry	2570:2577	Ministry	2570:2577	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	8	81	theme	primary	1134:1140	arg1	objective					1142:1150	The primary objective	1130:1150	The primary objective	1130:1150	The primary objective was to assess the diagnostic accuracy of FujiLAM compared with AlereLAM, against microbiological and composite reference standards (including clinical diagnoses).					
31155318	2	82	from	use	377:379	arg1	practice					393:400	clinical practice	384:400	clinical practice	384:400	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	2	83	dep	test	285:288	arg1	Alere					291:295	Alere	291:295	Alere	291:295	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	5	84	theme	diagnostic	686:695	arg1	study					706:710	this diagnostic accuracy study	681:710	this diagnostic accuracy study	681:710	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	2	85	theme	clinical	384:391	arg1	practice					393:400	clinical practice	384:400	clinical practice	384:400	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	5	86	theme	Town	813:816	arg1	Bank					781:784	FIND Specimen Bank	767:784	FIND Specimen Bank	767:784	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	12	87	theme	64·9	2070:2073	arg1	%					2074:2074	64·9% [50·1 to 76·7] for FujiLAM	2070:2101	64·9% [50·1 to 76·7] for FujiLAM	2070:2101	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	2	88	theme	available	248:256	arg1	test					285:288	The only commercially available tuberculosis point-of-care test	226:288	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM])	226:327	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	4	89	theme	AlereLAM	653:660	arg1	assay					662:666	the AlereLAM assay	649:666	the AlereLAM assay	649:666	We assessed the diagnostic accuracy of the FujiLAM assay for the detection of tuberculosis in hospital inpatients with HIV compared with the AlereLAM assay.					
31155318	11	90	theme	difference	1710:1719	arg1	AlereLAM					1700:1707	AlereLAM	1700:1707	AlereLAM (difference 28·1%)	1700:1726	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	11	90	theme	difference	1710:1719	arg1	%					1725:1725	difference 28·1%	1710:1725	difference 28·1%	1710:1725	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	5	91	theme	≥18	883:885	arg1	years					887:891	aged ≥18 years	878:891	aged ≥18 years	878:891	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	5	91	theme	≥18	883:885	arg1	inpatients					866:875	hospital inpatients	857:875	hospital inpatients (aged ≥18 years) with HIV	857:901	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	8	92	theme	diagnostic	1170:1179	arg1	accuracy					1181:1188	the diagnostic accuracy	1166:1188	the diagnostic accuracy of FujiLAM	1166:1199	The primary objective was to assess the diagnostic accuracy of FujiLAM compared with AlereLAM, against microbiological and composite reference standards (including clinical diagnoses).					
31155318	15	93	theme	National	2792:2799	arg1	Foundation					2810:2819	National Research Foundation	2792:2819	National Research Foundation	2792:2819	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	94	theme	Foreign	2582:2588	arg1	Affairs					2590:2596	Foreign Affairs	2582:2596	Foreign Affairs	2582:2596	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	12	95	theme	assays	2052:2057	arg1	lower					2063:2067	lower	2063:2067	lower	2063:2067	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	12	95	theme	assays	2052:2057	arg1	sensitivity					2032:2042	the sensitivity	2028:2042	the sensitivity of both assays	2028:2057	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	15	96	theme	International	2537:2549	arg1	Development					2551:2561	International Development	2537:2561	International Development	2537:2561	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	8	97	theme	reference	1263:1271	arg1	standards					1273:1281	microbiological and composite reference standards	1233:1281	microbiological and composite reference standards (including clinical diagnoses)	1233:1312	The primary objective was to assess the diagnostic accuracy of FujiLAM compared with AlereLAM, against microbiological and composite reference standards (including clinical diagnoses).					
31155318	13	98	theme	hospital	2348:2355	arg1	inpatients					2357:2366	hospital inpatients	2348:2366	hospital inpatients with HIV	2348:2375	INTERPRETATION In comparison to AlereLAM, FujiLAM offers superior diagnostic sensitivity, while maintaining specificity, and could transform rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV.					
31155318	2	99	theme	point-of-care	271:283	arg1	test					285:288	The only commercially available tuberculosis point-of-care test	226:288	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM])	226:327	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	12	100	dep	%	1974:1974	arg1	to					1982:1983	to	1982:1983	to	1982:1983	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	5	101	theme	hospital	857:864	arg1	years					887:891	aged ≥18 years	878:891	aged ≥18 years	878:891	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	5	101	theme	hospital	857:864	arg1	inpatients					866:875	hospital inpatients	857:875	hospital inpatients (aged ≥18 years) with HIV	857:901	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	9	102	dep	FINDINGS	1315:1322	arg1	May					1352:1354	May 3, 2018	1352:1362	May	1352:1354	FINDINGS Between April 18, 2018, and May 3, 2018, urine samples from 968 hospital inpatients with HIV were evaluated.					
31155318	9	102	dep	FINDINGS	1315:1322	arg1	FINDINGS					1315:1322	FINDINGS	1315:1322	FINDINGS	1315:1322	FINDINGS Between April 18, 2018, and May 3, 2018, urine samples from 968 hospital inpatients with HIV were evaluated.					
31155318	9	102	dep	FINDINGS	1315:1322	arg1	April					1332:1336	April	1332:1336	April	1332:1336	FINDINGS Between April 18, 2018, and May 3, 2018, urine samples from 968 hospital inpatients with HIV were evaluated.					
31155318	9	102	dep	FINDINGS	1315:1322	arg1	2018					1342:1345	2018	1342:1345	2018	1342:1345	FINDINGS Between April 18, 2018, and May 3, 2018, urine samples from 968 hospital inpatients with HIV were evaluated.					
31155318	2	103	dep	Alere	291:295	arg1	Determine					297:305	Determine	297:305	Determine TB LAM Ag [AlereLAM	297:325	The only commercially available tuberculosis point-of-care test (Alere Determine TB LAM Ag [AlereLAM]) has suboptimal sensitivity, which restricts its use in clinical practice.					
31155318	13	104	theme	tuberculosis	2321:2332	arg1	diagnosis					2334:2342	rapid point-of-care tuberculosis diagnosis	2301:2342	rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV	2301:2375	INTERPRETATION In comparison to AlereLAM, FujiLAM offers superior diagnostic sensitivity, while maintaining specificity, and could transform rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV.					
31155318	0	105	theme	diagnostic	79:88	arg1	study					99:103	a diagnostic accuracy study	77:103	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.	0:104	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.					
31155318	15	106	theme	Medical	2856:2862	arg1	Council					2873:2879	African Medical Research Council	2848:2879	African Medical Research Council	2848:2879	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	8	107	theme	clinical	1294:1301	arg1	diagnoses					1303:1311	clinical diagnoses	1294:1311	clinical diagnoses	1294:1311	The primary objective was to assess the diagnostic accuracy of FujiLAM compared with AlereLAM, against microbiological and composite reference standards (including clinical diagnoses).					
31155318	13	108	theme	point-of-care	2307:2319	arg1	diagnosis					2334:2342	rapid point-of-care tuberculosis diagnosis	2301:2342	rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV	2301:2375	INTERPRETATION In comparison to AlereLAM, FujiLAM offers superior diagnostic sensitivity, while maintaining specificity, and could transform rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV.					
31155318	1	109	with	people	153:158	arg1	HIV					165:167	HIV	165:167	HIV	165:167	BACKGROUND Most tuberculosis-related deaths in people with HIV could be prevented with earlier diagnosis and treatment.					
31155318	5	110	theme	prospective	928:938	arg1	studies					947:953	three independent prospective cohort studies	910:953	three independent prospective cohort studies done at two South African hospitals	910:989	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	13	111	from	INTERPRETATION	2160:2173	arg1	comparison					2178:2187	comparison	2178:2187	comparison to AlereLAM, FujiLAM	2178:2208	INTERPRETATION In comparison to AlereLAM, FujiLAM offers superior diagnostic sensitivity, while maintaining specificity, and could transform rapid point-of-care tuberculosis diagnosis for hospital inpatients with HIV.					
31155318	5	112	with	inpatients	866:875	arg1	HIV					899:901	HIV	899:901	HIV	899:901	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	5	113	theme	biobanked	725:733	arg1	samples					741:747	biobanked urine samples	725:747	biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals	725:989	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	5	114	dep	METHODS	669:675	arg1	assessed					716:723	assessed	716:723	assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals	716:989	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	3	115	theme	FujiLAM	438:444	arg1	assay					447:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay has been developed to improve the sensitivity of AlereLAM.					
31155318	0	116	theme	lipoarabinomannan	6:22	arg1	test					51:54	Novel lipoarabinomannan point-of-care tuberculosis test	0:54	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.	0:104	Novel lipoarabinomannan point-of-care tuberculosis test for people with HIV: a diagnostic accuracy study.					
31155318	6	117	theme	AlereLAM	1036:1043	arg1	assays					1045:1050	FujiLAM and AlereLAM assays	1024:1050	FujiLAM and AlereLAM assays	1024:1050	Urine samples were tested using FujiLAM and AlereLAM assays.					
31155318	4	118	with	inpatients	615:624	arg1	HIV					631:633	HIV	631:633	HIV	631:633	We assessed the diagnostic accuracy of the FujiLAM assay for the detection of tuberculosis in hospital inpatients with HIV compared with the AlereLAM assay.					
31155318	3	119	theme	LAM	433:435	arg1	assay					447:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay has been developed to improve the sensitivity of AlereLAM.					
31155318	7	120	theme	test	1116:1119	arg1	results					1121:1127	other test results	1110:1127	other test results	1110:1127	The conduct and reporting of each test was done blind to other test results.					
31155318	6	121	theme	FujiLAM	1024:1030	arg1	assays					1045:1050	FujiLAM and AlereLAM assays	1024:1050	FujiLAM and AlereLAM assays	1024:1050	Urine samples were tested using FujiLAM and AlereLAM assays.					
31155318	11	122	theme	microbiological	1556:1570	arg1	reference					1572:1580	the microbiological reference	1552:1580	the microbiological reference standard	1552:1589	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	4	123	theme	FujiLAM	555:561	arg1	assay					563:567	the FujiLAM assay	551:567	the FujiLAM assay	551:567	We assessed the diagnostic accuracy of the FujiLAM assay for the detection of tuberculosis in hospital inpatients with HIV compared with the AlereLAM assay.					
31155318	12	124	dep	higher	1926:1931	arg1	%					1938:1938	95·7% [92·0 to 98·0] for FujiLAM	1934:1965	95·7% [92·0 to 98·0] for FujiLAM	1934:1965	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	12	124	dep	higher	1926:1931	arg1	%					1974:1974	98·2% [95·7 to 99·6] for AlereLAM	1970:2002	98·2% [95·7 to 99·6] for AlereLAM	1970:2002	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	14	125	theme	use	2432:2434	arg1	settings					2411:2418	settings	2411:2418	settings of intended use	2411:2434	The applicability of FujiLAM for settings of intended use requires prospective assessment.					
31155318	3	126	theme	SILVAMP	422:428	arg1	assay					447:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay has been developed to improve the sensitivity of AlereLAM.					
31155318	1	127	dep	earlier	193:199	arg1	treatment					215:223	treatment	215:223	treatment	215:223	BACKGROUND Most tuberculosis-related deaths in people with HIV could be prevented with earlier diagnosis and treatment.					
31155318	1	127	dep	earlier	193:199	arg1	diagnosis					201:209	diagnosis	201:209	diagnosis	201:209	BACKGROUND Most tuberculosis-related deaths in people with HIV could be prevented with earlier diagnosis and treatment.					
31155318	3	128	theme	novel	407:411	arg1	assay					447:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	403:451	The novel Fujifilm SILVAMP TB LAM (FujiLAM) assay has been developed to improve the sensitivity of AlereLAM.					
31155318	4	129	theme	diagnostic	528:537	arg1	accuracy					539:546	the diagnostic accuracy	524:546	the diagnostic accuracy of the FujiLAM assay for the detection of tuberculosis	524:601	We assessed the diagnostic accuracy of the FujiLAM assay for the detection of tuberculosis in hospital inpatients with HIV compared with the AlereLAM assay.					
31155318	10	130	theme	tuberculosis	1479:1490	arg1	prevalence					1437:1446	The prevalence	1433:1446	The prevalence of microbiologically-confirmed tuberculosis	1433:1490	The prevalence of microbiologically-confirmed tuberculosis was 62% and the median CD4 count was 86 cells per μL.					
31155318	10	130	theme	tuberculosis	1479:1490	arg1	%					1498:1498	62%	1496:1498	62%	1496:1498	The prevalence of microbiologically-confirmed tuberculosis was 62% and the median CD4 count was 86 cells per μL.					
31155318	12	131	theme	98·2	1970:1973	arg1	%					1974:1974	98·2% [95·7 to 99·6] for AlereLAM	1970:2002	98·2% [95·7 to 99·6] for AlereLAM	1970:2002	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	11	132	dep	94·4	1788:1791	arg1	to					1785:1786	to	1785:1786	to	1785:1786	Using the microbiological reference standard, the estimated sensitivity of FujiLAM was 70·4% (95% CI 53·0 to 83·1) compared with 42·3% (31·7 to 51·8) for AlereLAM (difference 28·1%) and the estimated specificity of FujiLAM was 90·8% (86·0 to 94·4) and 95·0% (87·7-98·8) for AlereLAM (difference -4·2%).					
31155318	8	133	dep	standards	1273:1281	arg1	including					1284:1292	including	1284:1292	including clinical diagnoses	1284:1311	The primary objective was to assess the diagnostic accuracy of FujiLAM compared with AlereLAM, against microbiological and composite reference standards (including clinical diagnoses).					
31155318	15	134	theme	Science	2765:2771	arg1	Department					2751:2760	Department	2751:2760	Department of Science	2751:2771	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	134	theme	Science	2765:2771	arg1	Foundation					2810:2819	National Research Foundation	2792:2819	National Research Foundation	2792:2819	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	134	theme	Science	2765:2771	arg1	Trust					2744:2748	Foreign Affairs and Trade, Wellcome Trust	2708:2748	Foreign Affairs and Trade, Wellcome Trust	2708:2748	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	134	theme	Science	2765:2771	arg1	Technology					2777:2786	Technology	2777:2786	Technology	2777:2786	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	4	135	theme	tuberculosis	590:601	arg1	detection					577:585	the detection	573:585	the detection of tuberculosis	573:601	We assessed the diagnostic accuracy of the FujiLAM assay for the detection of tuberculosis in hospital inpatients with HIV compared with the AlereLAM assay.					
31155318	12	136	theme	95·7	1934:1937	arg1	%					1938:1938	95·7% [92·0 to 98·0] for FujiLAM	1934:1965	95·7% [92·0 to 98·0] for FujiLAM	1934:1965	Against the composite reference standard, the specificity of both assays was higher (95·7% [92·0 to 98·0] for FujiLAM vs 98·2% [95·7 to 99·6] for AlereLAM; difference -2·5%), but the sensitivity of both assays was lower (64·9% [50·1 to 76·7] for FujiLAM vs 38·2% [28·1 to 47·3] for AlereLAM; difference 26·7%).					
31155318	5	137	theme	South	967:971	arg1	hospitals					981:989	two South African hospitals	963:989	two South African hospitals	963:989	METHODS For this diagnostic accuracy study, we assessed biobanked urine samples obtained from the FIND Specimen Bank and the University of Cape Town Biobank, which had been collected from hospital inpatients (aged ≥18 years) with HIV during three independent prospective cohort studies done at two South African hospitals.					
31155318	4	138	theme	hospital	606:613	arg1	inpatients					615:624	hospital inpatients	606:624	hospital inpatients with HIV	606:633	We assessed the diagnostic accuracy of the FujiLAM assay for the detection of tuberculosis in hospital inpatients with HIV compared with the AlereLAM assay.					
31155318	15	139	theme	FUNDING	2469:2475	arg1	Fund					2513:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund	2469:2516	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	139	theme	FUNDING	2469:2475	arg1	Department					2522:2531	Department	2522:2531	Department	2522:2531	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	139	theme	FUNDING	2469:2475	arg1	Bill					2599:2602	Bill	2599:2602	Bill	2599:2602	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31155318	15	139	theme	FUNDING	2469:2475	arg1	Ministry					2570:2577	Ministry	2570:2577	Ministry	2570:2577	FUNDING Global Health Innovative Technology Fund, UK Department for International Development, Dutch Ministry of Foreign Affairs, Bill & Melinda Gates Foundation, German Federal Ministry of Education and Research, Australian Department of Foreign Affairs and Trade, Wellcome Trust, Department of Science and Technology and National Research Foundation of South Africa, and South African Medical Research Council.					
31253299	0	0	theme	beverages	55:63	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?	0:139	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	5	1	theme	inhibitory	619:628	arg1	capacity					630:637	their strong inhibitory capacity	606:637	their strong inhibitory capacity	606:637	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	7	2	theme	remarkable	878:887	arg1	effect					889:894	a remarkable effect	876:894	a remarkable effect	876:894	Lemon juice had a remarkable effect, completely interrupting gastric amylolysis by salivary amylase via a preliminary acidification of gastric contents.					
31253299	4	3	from	condiments	365:374	arg1	amylolysis					451:460	salivary and pancreatic amylolysis	427:460	salivary and pancreatic amylolysis	427:460	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	4	4	theme	10	486:487	arg1	%					488:488	%	488:488	%	488:488	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	0	5	theme	other	49:53	arg1	beverages					55:63	other beverages	49:63	other beverages	49:63	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	0	6	from	effect	11:16	arg1	amylases					92:99	salivary and pancreatic amylases:	68:100	salivary and pancreatic amylases: What impact on bread starch digestion	68:138	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	8	7	theme	dietary	1089:1095	arg1	strategies					1097:1106	dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo	1089:1189	dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo	1089:1189	These results provide a strong biochemical rationale for the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo.					
31253299	7	8	theme	gastric	995:1001	arg1	contents					1003:1010	gastric contents	995:1010	gastric contents	995:1010	Lemon juice had a remarkable effect, completely interrupting gastric amylolysis by salivary amylase via a preliminary acidification of gastric contents.					
31253299	4	9	dep	determine	325:333	arg1	1					319:319	1	319:319	1	319:319	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	6	10	theme	intestinal	842:851	arg1	phase					853:857	the intestinal phase	838:857	the intestinal phase	838:857	Compared to water (control), the effect of black tea was limited to a ≈20% reduction of released oligosaccharides during the intestinal phase.					
31253299	0	11	theme	salivary	68:75	arg1	amylases					92:99	salivary and pancreatic amylases:	68:100	salivary and pancreatic amylases: What impact on bread starch digestion	68:138	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	6	12	theme	black	760:764	arg1	tea					766:768	black tea	760:768	black tea	760:768	Compared to water (control), the effect of black tea was limited to a ≈20% reduction of released oligosaccharides during the intestinal phase.					
31253299	4	13	from	impact	339:344	arg1	amylolysis					451:460	salivary and pancreatic amylolysis	427:460	salivary and pancreatic amylolysis	427:460	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	7	14	theme	preliminary	966:976	arg1	acidification					978:990	a preliminary acidification	964:990	a preliminary acidification of gastric contents	964:1010	Lemon juice had a remarkable effect, completely interrupting gastric amylolysis by salivary amylase via a preliminary acidification of gastric contents.					
31253299	0	15	theme	pancreatic	81:90	arg1	amylases					92:99	salivary and pancreatic amylases:	68:100	salivary and pancreatic amylases: What impact on bread starch digestion	68:138	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	8	16	theme	strong	1037:1042	arg1	rationale					1056:1064	a strong biochemical rationale	1035:1064	a strong biochemical rationale for the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo	1035:1189	These results provide a strong biochemical rationale for the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo.					
31253299	8	17	theme	glycaemic	1123:1131	arg1	impact					1133:1138	the glycaemic impact	1119:1138	the glycaemic impact of starch-rich meals which could be tested in vivo	1119:1189	These results provide a strong biochemical rationale for the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo.					
31253299	7	18	theme	contents	1003:1010	arg1	acidification					978:990	a preliminary acidification	964:990	a preliminary acidification of gastric contents	964:1010	Lemon juice had a remarkable effect, completely interrupting gastric amylolysis by salivary amylase via a preliminary acidification of gastric contents.					
31253299	5	19	theme	dynamicoro-gastro-intestinal	668:695	arg1	digestion					697:705	dynamicoro-gastro-intestinal digestion	668:705	dynamicoro-gastro-intestinal digestion of bread	668:714	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	7	20	contain	had	872:874	arg1	juice					866:870	Lemon juice	860:870	Lemon juice	860:870	Lemon juice had a remarkable effect, completely interrupting gastric amylolysis by salivary amylase via a preliminary acidification of gastric contents.					
31253299	7	20	contain	had	872:874	arg2	effect					889:894	a remarkable effect	876:894	a remarkable effect	876:894	Lemon juice had a remarkable effect, completely interrupting gastric amylolysis by salivary amylase via a preliminary acidification of gastric contents.					
31253299	2	21	theme	slow	269:272	arg1	digestion					286:294	slow down starch digestion	269:294	slow down starch digestion	269:294	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	1	22	theme	dynamic	143:149	arg1	study					160:164	A dynamic in vitro study	141:164	A dynamic in vitro study	141:164	A dynamic in vitro study.					
31253299	0	23	theme	Inhibitory	0:9	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?	0:139	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	6	24	theme	oligosaccharides	814:829	arg1	reduction					792:800	a ≈20% reduction	785:800	a ≈20% reduction of released oligosaccharides	785:829	Compared to water (control), the effect of black tea was limited to a ≈20% reduction of released oligosaccharides during the intestinal phase.					
31253299	7	25	theme	salivary	943:950	arg1	amylase					952:958	salivary amylase	943:958	salivary amylase	943:958	Lemon juice had a remarkable effect, completely interrupting gastric amylolysis by salivary amylase via a preliminary acidification of gastric contents.					
31253299	1	26	theme	in	151:152	arg1	study					160:164	A dynamic in vitro study	141:164	A dynamic in vitro study	141:164	A dynamic in vitro study.					
31253299	0	27	theme	black	21:25	arg1	tea					27:29	black tea	21:29	black tea	21:29	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	6	28	theme	released	805:812	arg1	oligosaccharides					814:829	released oligosaccharides	805:829	released oligosaccharides	805:829	Compared to water (control), the effect of black tea was limited to a ≈20% reduction of released oligosaccharides during the intestinal phase.					
31253299	5	29	theme	black	566:570	arg1	tea					572:574	one black tea	562:574	one black tea	562:574	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	2	30	dep	properties	180:189	arg1	composition					214:224	polyphenolic composition	201:224	polyphenolic composition	201:224	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	2	30	dep	properties	180:189	arg1	ex.					192:194	ex.	192:194	ex.	192:194	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	5	31	theme	tea	572:574	arg1	effect					552:557	the effect	548:557	the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis	548:659	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	4	32	theme	experimental	504:515	arg1	conditions					517:526	our experimental conditions	500:526	our experimental conditions	500:526	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	4	33	dep	%	488:488	arg1	to					490:491	to	490:491	to	490:491	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	2	34	dep	ex.	192:194	arg1	pH					197:198	pH	197:198	pH	197:198	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	0	35	theme	tea	27:29	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?	0:139	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	4	36	theme	salivary	427:434	arg1	amylolysis					451:460	salivary and pancreatic amylolysis	427:460	salivary and pancreatic amylolysis	427:460	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	5	37	theme	lemon	580:584	arg1	juice					586:590	lemon juice	580:590	lemon juice	580:590	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	8	38	theme	meals	1155:1159	arg1	impact					1133:1138	the glycaemic impact	1119:1138	the glycaemic impact of starch-rich meals which could be tested in vivo	1119:1189	These results provide a strong biochemical rationale for the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo.					
31253299	1	39	dep	in	151:152	arg1	vitro					154:158	vitro	154:158	vitro	154:158	A dynamic in vitro study.					
31253299	8	40	theme	starch-rich	1143:1153	arg1	meals					1155:1159	starch-rich meals	1143:1159	starch-rich meals which could be tested in vivo	1143:1189	These results provide a strong biochemical rationale for the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo.					
31253299	0	41	theme	juice	38:42	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?	0:139	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	5	42	theme	juice	586:590	arg1	effect					552:557	the effect	548:557	the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis	548:659	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	0	43	theme	bread	117:121	arg1	digestion					130:138	bread starch digestion	117:138	bread starch digestion	117:138	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	5	44	theme	strong	612:617	arg1	capacity					630:637	their strong inhibitory capacity	606:637	their strong inhibitory capacity	606:637	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	5	45	dep	investigate	536:546	arg1	2					530:530	2	530:530	2	530:530	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	0	46	theme	lemon	32:36	arg1	juice					38:42	lemon juice	32:42	lemon juice	32:42	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	4	47	theme	pancreatic	440:449	arg1	amylolysis					451:460	salivary and pancreatic amylolysis	427:460	salivary and pancreatic amylolysis	427:460	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	7	48	theme	gastric	921:927	arg1	amylolysis					929:938	gastric amylolysis	921:938	gastric amylolysis by salivary amylase	921:958	Lemon juice had a remarkable effect, completely interrupting gastric amylolysis by salivary amylase via a preliminary acidification of gastric contents.					
31253299	2	49	theme	Certain	167:173	arg1	properties					180:189	Certain food properties	167:189	Certain food properties (ex.: pH, polyphenolic composition)	167:225	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	2	50	theme	food	175:178	arg1	properties					180:189	Certain food properties	167:189	Certain food properties (ex.: pH, polyphenolic composition)	167:225	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	6	51	theme	%	790:790	arg1	reduction					792:800	a ≈20% reduction	785:800	a ≈20% reduction of released oligosaccharides	785:829	Compared to water (control), the effect of black tea was limited to a ≈20% reduction of released oligosaccharides during the intestinal phase.					
31253299	4	52	theme	of21	346:349	arg1	beverages					351:359	of21 beverages	346:359	of21 beverages	346:359	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	2	53	theme	polyphenolic	201:212	arg1	composition					214:224	polyphenolic composition	201:224	polyphenolic composition	201:224	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	2	53	theme	polyphenolic	201:212	arg1	ex.					192:194	ex.	192:194	ex.	192:194	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	7	54	theme	Lemon	860:864	arg1	juice					866:870	Lemon juice	860:870	Lemon juice	860:870	Lemon juice had a remarkable effect, completely interrupting gastric amylolysis by salivary amylase via a preliminary acidification of gastric contents.					
31253299	2	55	theme	starch	279:284	arg1	digestion					286:294	slow down starch digestion	269:294	slow down starch digestion	269:294	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	6	56	theme	tea	766:768	arg1	effect					750:755	the effect	746:755	the effect of black tea	746:768	Compared to water (control), the effect of black tea was limited to a ≈20% reduction of released oligosaccharides during the intestinal phase.					
31253299	0	57	from	impact	107:112	arg1	digestion					130:138	bread starch digestion	117:138	bread starch digestion	117:138	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	0	58	theme	starch	123:128	arg1	digestion					130:138	bread starch digestion	117:138	bread starch digestion	117:138	Inhibitory effect of black tea, lemon juice, and other beverages on salivary and pancreatic amylases: What impact on bread starch digestion?					
31253299	8	59	theme	biochemical	1044:1054	arg1	rationale					1056:1064	a strong biochemical rationale	1035:1064	a strong biochemical rationale for the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo	1035:1189	These results provide a strong biochemical rationale for the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo.					
31253299	2	60	theme	down	274:277	arg1	digestion					286:294	slow down starch digestion	269:294	slow down starch digestion	269:294	Certain food properties (ex.: pH, polyphenolic composition) can inhibitdigestive amylases and thereby slow down starch digestion.					
31253299	8	61	theme	strategies	1097:1106	arg1	development					1074:1084	the development	1070:1084	the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo	1070:1189	These results provide a strong biochemical rationale for the development of dietary strategies to improve the glycaemic impact of starch-rich meals which could be tested in vivo.					
31253299	4	62	theme	100	493:495	arg1	%					488:488	%	488:488	%	488:488	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	5	63	theme	bread	710:714	arg1	digestion					697:705	dynamicoro-gastro-intestinal digestion	668:705	dynamicoro-gastro-intestinal digestion of bread	668:714	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	5	64	theme	starch	643:648	arg1	hydrolysis					650:659	starch hydrolysis	643:659	starch hydrolysis	643:659	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
31253299	4	65	theme	lemon	412:416	arg1	juice					418:422	lemon juice	412:422	lemon juice	412:422	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	4	66	dep	condiments	365:374	arg1	coffees					377:383	coffees	377:383	coffees	377:383	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	4	66	dep	condiments	365:374	arg1	vinegars					399:406	vinegars	399:406	vinegars	399:406	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	4	66	dep	condiments	365:374	arg1	juice					418:422	lemon juice	412:422	lemon juice	412:422	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	4	66	dep	condiments	365:374	arg1	wines					392:396	wines	392:396	wines	392:396	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	4	66	dep	condiments	365:374	arg1	teas					386:389	teas	386:389	teas	386:389	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	4	67	dep	impact	339:344	arg1	beverages					351:359	of21 beverages	346:359	of21 beverages	346:359	(1) To determine the impact of21 beverages and condiments (coffees, teas, wines, vinegars and lemon juice)on salivary and pancreatic amylolysis: inhibition ranged from 10% to 100%in our experimental conditions.					
31253299	5	68	from	effect	552:557	arg1	hydrolysis					650:659	starch hydrolysis	643:659	starch hydrolysis	643:659	(2) To investigate the effect of one black tea and lemon juice (selected for their strong inhibitory capacity) on starch hydrolysis during dynamicoro-gastro-intestinal digestion of bread.					
29799652	2	0	theme	higher	509:514	arg1	temperature					522:532	higher water temperature	509:532	higher water temperature	509:532	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	4	1	theme	FM	931:932	arg1	levels					921:926	varying levels	913:926	varying levels of FM and prebiotic supplementation	913:962	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	3	2	theme	hindgut	592:598	arg1	samples					607:613	hindgut faecal samples	592:613	hindgut faecal samples	592:613	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	6	3	theme	greater	1296:1302	arg1	effect					1304:1309	a greater effect	1294:1309	a greater effect on LAB colonization of the gut in Atlantic salmon	1294:1359	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	4	4	theme	prebiotic	938:946	arg1	supplementation					948:962	prebiotic supplementation	938:962	prebiotic supplementation	938:962	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	3	5	theme	Atlantic	635:642	arg1	salmon					644:649	Atlantic salmon	635:649	Atlantic salmon	635:649	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	4	6	theme	increased	1007:1015	arg1	temperatures					1023:1034	increased water temperatures	1007:1034	increased water temperatures	1007:1034	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	4	7	dep	levels	921:926	arg1	response					901:908	response	901:908	response	901:908	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	3	8	contain	containing	742:751	arg1	diets					736:740	four trial diets	725:740	four trial diets containing either high or low FM inclusion levels	725:790	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	3	8	contain	containing	742:751	arg2	levels					785:790	either high or low FM inclusion levels	753:790	either high or low FM inclusion levels	753:790	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	3	9	theme	commercial	658:667	arg1	farm					674:677	a commercial fish farm	656:677	a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics	656:817	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	7	10	theme	salmon	1472:1477	arg1	health					1479:1484	salmon health	1472:1484	salmon health in aquaculture industry	1472:1508	SIGNIFICANCE AND IMPACT OF THE STUDY This study highlights the importance of the new fish feeds for promoting salmon health in aquaculture industry.					
29799652	0	11	theme	Atlantic	116:123	arg1	salmon					125:130	farmed Tasmanian Atlantic salmon	99:130	farmed Tasmanian Atlantic salmon (Salmo salar L.)	99:147	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	0	11	theme	Atlantic	116:123	arg1	salar					139:143	Salmo salar L.	133:146	Salmo salar L.	133:146	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	1	12	theme	seasonal	171:178	arg1	temperature					180:190	seasonal temperature	171:190	seasonal temperature	171:190	AIMS Factors such as seasonal temperature and diet components, for example, fishmeal (FM) inclusion, can influence the composition of the gut microbiota of fish.					
29799652	6	13	dep	CONCLUSIONS	1176:1186	arg1	indicates					1198:1206	indicates	1198:1206	indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon	1198:1359	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	2	14	theme	salmon	452:457	arg1	LAB					417:419	LAB	417:419	LAB	417:419	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	2	14	theme	salmon	452:457	arg1	bacteria					407:414	particular lactic acid bacteria	384:414	particular lactic acid bacteria (LAB)	384:420	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	2	15	from	bacteria	407:414	arg1	response					462:469	response	462:469	response to different diets	462:488	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	7	16	from	health	1479:1484	arg1	industry					1501:1508	aquaculture industry	1489:1508	aquaculture industry	1489:1508	SIGNIFICANCE AND IMPACT OF THE STUDY This study highlights the importance of the new fish feeds for promoting salmon health in aquaculture industry.					
29799652	4	17	dep	Overall	820:826	arg1	there					828:832	there	828:832	there	828:832	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	7	18	theme	fish	1447:1450	arg1	importance					1425:1434	the importance	1421:1434	the importance of the new fish	1421:1450	SIGNIFICANCE AND IMPACT OF THE STUDY This study highlights the importance of the new fish feeds for promoting salmon health in aquaculture industry.					
29799652	3	19	theme	diets	736:740	arg1	diets					736:740	four trial diets	725:740	four trial diets containing either high or low FM inclusion levels	725:790	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	3	19	theme	diets	736:740	arg1	one					718:720	one	718:720	one	718:720	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	2	20	theme	different	474:482	arg1	diets					484:488	different diets	474:488	different diets	474:488	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	3	21	theme	Tasmania	699:706	arg1	south					682:686	south	682:686	south	682:686	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	0	22	dep	salar	139:143	arg1	L					145:145	L	145:145	Salmo salar L.	133:146	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	6	23	theme	amounts	1237:1243	arg1	inclusion					1217:1225	the inclusion	1213:1225	the inclusion of higher amounts of FM rather than the addition of prebiotics	1213:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	4	24	theme	cultivatable	863:874	arg1	populations					886:896	the cultivatable bacterial populations	859:896	the cultivatable bacterial populations	859:896	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	3	25	from	farm	674:677	arg1	south					682:686	south	682:686	south	682:686	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	0	26	theme	hindgut	70:76	arg1	microbiota					85:94	the hindgut faecal microbiota	66:94	the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.)	66:147	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	1	27	theme	gut	288:290	arg1	microbiota					292:301	the gut microbiota	284:301	the gut microbiota of fish	284:309	AIMS Factors such as seasonal temperature and diet components, for example, fishmeal (FM) inclusion, can influence the composition of the gut microbiota of fish.					
29799652	2	28	theme	Tasmanian	433:441	arg1	salmon					452:457	farmed Tasmanian Atlantic salmon	426:457	farmed Tasmanian Atlantic salmon in response to different diets	426:488	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	4	29	theme	little	838:843	arg1	difference					845:854	little difference	838:854	little difference in the cultivatable bacterial populations	838:896	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	0	30	theme	farmed	99:104	arg1	salmon					125:130	farmed Tasmanian Atlantic salmon	99:130	farmed Tasmanian Atlantic salmon (Salmo salar L.)	99:147	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	0	30	theme	farmed	99:104	arg1	salar					139:143	Salmo salar L.	133:146	Salmo salar L.	133:146	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	3	31	theme	FM	772:773	arg1	levels					785:790	either high or low FM inclusion levels	753:790	either high or low FM inclusion levels	753:790	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	4	32	theme	varying	913:919	arg1	levels					921:926	varying levels	913:926	varying levels of FM and prebiotic supplementation	913:962	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	6	33	theme	prebiotics	1279:1288	arg1	addition					1267:1274	the addition	1263:1274	FM rather than the addition of prebiotics	1248:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	7	34	theme	aquaculture	1489:1499	arg1	industry					1501:1508	aquaculture industry	1489:1508	aquaculture industry	1489:1508	SIGNIFICANCE AND IMPACT OF THE STUDY This study highlights the importance of the new fish feeds for promoting salmon health in aquaculture industry.					
29799652	2	35	theme	bacterial	358:366	arg1	populations					368:378	the gut bacterial populations	350:378	the gut bacterial populations	350:378	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	5	36	theme	low	1162:1164	arg1	diets					1169:1173	the low FM diets	1158:1173	the low FM diets	1158:1173	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	6	37	theme	addition	1267:1274	arg1	FM					1248:1249	FM	1248:1249	FM rather than the addition of prebiotics	1248:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	6	37	theme	addition	1267:1274	arg1	amounts					1237:1243	higher amounts	1230:1243	higher amounts of FM rather than the addition of prebiotics	1230:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	6	37	theme	addition	1267:1274	arg1	addition					1267:1274	the addition	1263:1274	FM rather than the addition of prebiotics	1248:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	2	38	theme	acid	402:405	arg1	LAB					417:419	LAB	417:419	LAB	417:419	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	2	38	theme	acid	402:405	arg1	bacteria					407:414	particular lactic acid bacteria	384:414	particular lactic acid bacteria (LAB)	384:420	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	0	39	theme	inclusion	23:31	arg1	effect					4:9	The effect	0:9	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).	0:148	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	4	40	theme	bacterial	876:884	arg1	populations					886:896	the cultivatable bacterial populations	859:896	the cultivatable bacterial populations	859:896	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	2	41	theme	particular	384:393	arg1	LAB					417:419	LAB	417:419	LAB	417:419	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	2	41	theme	particular	384:393	arg1	bacteria					407:414	particular lactic acid bacteria	384:414	particular lactic acid bacteria (LAB)	384:420	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	0	42	theme	prebiotic	37:45	arg1	supplementation					47:61	prebiotic supplementation	37:61	prebiotic supplementation	37:61	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	1	43	dep	temperature	180:190	arg1	components					201:210	components	201:210	components	201:210	AIMS Factors such as seasonal temperature and diet components, for example, fishmeal (FM) inclusion, can influence the composition of the gut microbiota of fish.					
29799652	2	44	from	changes	339:345	arg1	populations					368:378	the gut bacterial populations	350:378	the gut bacterial populations	350:378	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	6	45	theme	LAB	1314:1316	arg1	colonization					1318:1329	LAB colonization	1314:1329	LAB colonization of the gut in Atlantic salmon	1314:1359	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	5	46	theme	LAB	1110:1112	arg1	presence					1098:1105	the presence	1094:1105	the presence of LAB in January	1094:1123	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	6	47	from	effect	1304:1309	arg1	colonization					1318:1329	LAB colonization	1314:1329	LAB colonization of the gut in Atlantic salmon	1314:1359	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	6	48	theme	higher	1230:1235	arg1	FM					1248:1249	FM	1248:1249	FM rather than the addition of prebiotics	1248:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	6	48	theme	higher	1230:1235	arg1	amounts					1237:1243	higher amounts	1230:1243	higher amounts of FM rather than the addition of prebiotics	1230:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	6	48	theme	higher	1230:1235	arg1	addition					1267:1274	the addition	1263:1274	FM rather than the addition of prebiotics	1248:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	5	49	theme	FM	1076:1077	arg1	diet					1079:1082	the high FM diet	1067:1082	the high FM diet	1067:1082	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	2	50	theme	temperature	522:532	arg1	periods					498:504	periods	498:504	periods of higher water temperature	498:532	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	4	51	theme	supplementation	948:962	arg1	levels					921:926	varying levels	913:926	varying levels of FM and prebiotic supplementation	913:962	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	3	52	theme	faecal	600:605	arg1	samples					607:613	hindgut faecal samples	592:613	hindgut faecal samples	592:613	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	4	53	theme	water	1017:1021	arg1	temperatures					1023:1034	increased water temperatures	1007:1034	increased water temperatures	1007:1034	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	6	54	theme	FM	1248:1249	arg1	FM					1248:1249	FM	1248:1249	FM rather than the addition of prebiotics	1248:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	6	54	theme	FM	1248:1249	arg1	amounts					1237:1243	higher amounts	1230:1243	higher amounts of FM rather than the addition of prebiotics	1230:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	6	54	theme	FM	1248:1249	arg1	addition					1267:1274	the addition	1263:1274	FM rather than the addition of prebiotics	1248:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	2	55	theme	water	516:520	arg1	temperature					522:532	higher water temperature	509:532	higher water temperature	509:532	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	3	56	theme	fish	669:672	arg1	farm					674:677	a commercial fish farm	656:677	a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics	656:817	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	0	57	theme	salmon	125:130	arg1	microbiota					85:94	the hindgut faecal microbiota	66:94	the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.)	66:147	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	6	58	theme	Atlantic	1345:1352	arg1	salmon					1354:1359	Atlantic salmon	1345:1359	Atlantic salmon	1345:1359	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	0	59	theme	Salmo	133:137	arg1	salmon					125:130	farmed Tasmanian Atlantic salmon	99:130	farmed Tasmanian Atlantic salmon (Salmo salar L.)	99:147	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	0	59	theme	Salmo	133:137	arg1	salar					139:143	Salmo salar L.	133:146	Salmo salar L.	133:146	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	6	60	theme	gut	1338:1340	arg1	colonization					1318:1329	LAB colonization	1314:1329	LAB colonization of the gut in Atlantic salmon	1314:1359	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	7	61	theme	new	1443:1445	arg1	fish					1447:1450	the new fish	1439:1450	the new fish	1439:1450	SIGNIFICANCE AND IMPACT OF THE STUDY This study highlights the importance of the new fish feeds for promoting salmon health in aquaculture industry.					
29799652	2	62	theme	Atlantic	443:450	arg1	salmon					452:457	farmed Tasmanian Atlantic salmon	426:457	farmed Tasmanian Atlantic salmon in response to different diets	426:488	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	5	63	attach	presence	1098:1105	arg1	January					1117:1123	January	1117:1123	January	1117:1123	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	5	63	attach	presence	1098:1105	arg2	LAB					1110:1112	LAB	1110:1112	LAB	1110:1112	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	5	64	from	presence	1098:1105	arg1	January					1117:1123	January	1117:1123	January	1117:1123	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	1	65	dep	example	217:223	arg1	inclusion					240:248	inclusion	240:248	inclusion	240:248	AIMS Factors such as seasonal temperature and diet components, for example, fishmeal (FM) inclusion, can influence the composition of the gut microbiota of fish.					
29799652	3	66	theme	trial	730:734	arg1	diets					736:740	four trial diets	725:740	four trial diets containing either high or low FM inclusion levels	725:790	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	0	67	theme	faecal	78:83	arg1	microbiota					85:94	the hindgut faecal microbiota	66:94	the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.)	66:147	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	3	68	theme	low	768:770	arg1	levels					785:790	either high or low FM inclusion levels	753:790	either high or low FM inclusion levels	753:790	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	7	69	theme	STUDY	1393:1397	arg1	SIGNIFICANCE					1362:1373	SIGNIFICANCE	1362:1373	SIGNIFICANCE	1362:1373	SIGNIFICANCE AND IMPACT OF THE STUDY This study highlights the importance of the new fish feeds for promoting salmon health in aquaculture industry.					
29799652	7	69	theme	STUDY	1393:1397	arg1	IMPACT					1379:1384	IMPACT	1379:1384	IMPACT	1379:1384	SIGNIFICANCE AND IMPACT OF THE STUDY This study highlights the importance of the new fish feeds for promoting salmon health in aquaculture industry.					
29799652	0	70	from	effect	4:9	arg1	microbiota					85:94	the hindgut faecal microbiota	66:94	the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.)	66:147	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	1	71	theme	microbiota	292:301	arg1	composition					269:279	the composition	265:279	the composition of the gut microbiota of fish	265:309	AIMS Factors such as seasonal temperature and diet components, for example, fishmeal (FM) inclusion, can influence the composition of the gut microbiota of fish.					
29799652	3	72	theme	high	760:763	arg1	levels					785:790	either high or low FM inclusion levels	753:790	either high or low FM inclusion levels	753:790	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	2	73	theme	farmed	426:431	arg1	salmon					452:457	farmed Tasmanian Atlantic salmon	426:457	farmed Tasmanian Atlantic salmon in response to different diets	426:488	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	5	74	located	detected	1146:1153	arg1	diets					1169:1173	the low FM diets	1158:1173	the low FM diets	1158:1173	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	5	74	located	detected	1146:1153	arg2	these					1131:1135	these	1131:1135	these	1131:1135	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	0	75	theme	Tasmanian	106:114	arg1	salmon					125:130	farmed Tasmanian Atlantic salmon	99:130	farmed Tasmanian Atlantic salmon (Salmo salar L.)	99:147	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	0	75	theme	Tasmanian	106:114	arg1	salar					139:143	Salmo salar L.	133:146	Salmo salar L.	133:146	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	1	76	theme	fish	306:309	arg1	microbiota					292:301	the gut microbiota	284:301	the gut microbiota of fish	284:309	AIMS Factors such as seasonal temperature and diet components, for example, fishmeal (FM) inclusion, can influence the composition of the gut microbiota of fish.					
29799652	3	77	theme	inclusion	775:783	arg1	levels					785:790	either high or low FM inclusion levels	753:790	either high or low FM inclusion levels	753:790	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	0	78	theme	fishmeal	14:21	arg1	inclusion					23:31	fishmeal inclusion	14:31	fishmeal inclusion	14:31	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	2	79	theme	gut	354:356	arg1	populations					368:378	the gut bacterial populations	350:378	the gut bacterial populations	350:378	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	5	80	theme	FM	1166:1167	arg1	diets					1169:1173	the low FM diets	1158:1173	the low FM diets	1158:1173	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	5	81	theme	high	1071:1074	arg1	diet					1079:1082	the high FM diet	1067:1082	the high FM diet	1067:1082	However, it was observed that the high FM diet supported the presence of LAB in January, when these were not detected in the low FM diets.					
29799652	6	82	from	colonization	1318:1329	arg1	salmon					1354:1359	Atlantic salmon	1345:1359	Atlantic salmon	1345:1359	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	2	83	theme	lactic	395:400	arg1	LAB					417:419	LAB	417:419	LAB	417:419	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	2	83	theme	lactic	395:400	arg1	bacteria					407:414	particular lactic acid bacteria	384:414	particular lactic acid bacteria (LAB)	384:420	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	0	84	theme	supplementation	47:61	arg1	effect					4:9	The effect	0:9	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).	0:148	The effect of fishmeal inclusion and prebiotic supplementation on the hindgut faecal microbiota of farmed Tasmanian Atlantic salmon (Salmo salar L.).					
29799652	1	85	dep	AIMS	150:153	arg1	influence					255:263	influence	255:263	can influence the composition of the gut microbiota of fish	251:309	AIMS Factors such as seasonal temperature and diet components, for example, fishmeal (FM) inclusion, can influence the composition of the gut microbiota of fish.					
29799652	6	86	contain	has	1290:1292	arg1	inclusion					1217:1225	the inclusion	1213:1225	the inclusion of higher amounts of FM rather than the addition of prebiotics	1213:1288	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	6	86	contain	has	1290:1292	arg2	effect					1304:1309	a greater effect	1294:1309	a greater effect on LAB colonization of the gut in Atlantic salmon	1294:1359	CONCLUSIONS Our study indicates that the inclusion of higher amounts of FM rather than the addition of prebiotics has a greater effect on LAB colonization of the gut in Atlantic salmon.					
29799652	2	87	from	salmon	452:457	arg1	response					462:469	response	462:469	response to different diets	462:488	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	7	88	dep	SIGNIFICANCE	1362:1373	arg1	highlights					1410:1419	highlights	1410:1419	highlights the importance of the new fish	1410:1450	SIGNIFICANCE AND IMPACT OF THE STUDY This study highlights the importance of the new fish feeds for promoting salmon health in aquaculture industry.					
29799652	4	89	theme	LAB	970:972	arg1	counts					974:979	LAB counts	970:979	LAB counts	970:979	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
29799652	2	90	from	response	462:469	arg1	LAB					417:419	LAB	417:419	LAB	417:419	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	2	90	from	response	462:469	arg1	bacteria					407:414	particular lactic acid bacteria	384:414	particular lactic acid bacteria (LAB)	384:420	In this study, we examined changes in the gut bacterial populations, in particular lactic acid bacteria (LAB), of farmed Tasmanian Atlantic salmon in response to different diets, during periods of higher water temperature.					
29799652	3	91	dep	December	563:570	arg1	samples					607:613	hindgut faecal samples	592:613	hindgut faecal samples	592:613	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	3	91	dep	December	563:570	arg1	2011					572:575	2011	572:575	December 2011	563:575	METHODS AND RESULTS Between December 2011 and March 2012 hindgut faecal samples were collected from Atlantic salmon from a commercial fish farm in south of Hobart, Tasmania, fed with one of four trial diets containing either high or low FM inclusion levels with or without prebiotics.					
29799652	4	92	from	difference	845:854	arg1	populations					886:896	the cultivatable bacterial populations	859:896	the cultivatable bacterial populations	859:896	Overall there was little difference in the cultivatable bacterial populations in response to varying levels of FM and prebiotic supplementation, with LAB counts decreasing in response to increased water temperatures.					
30701455	6	0	theme	male	756:759	arg1	mice					769:772	male C57BL/c mice	756:772	male C57BL/c mice	756:772	We created full-thickness skin wounds in male C57BL/c mice to evaluate the healing capacity of CLiS.					
30701455	1	1	theme	endogenous	178:187	arg1	mechanisms					196:205	endogenous repair mechanisms	178:205	endogenous repair mechanisms for in situ skin regeneration	178:235	Small molecules loaded into biological materials present a promising strategy for stimulating endogenous repair mechanisms for in situ skin regeneration.					
30701455	8	2	theme	wound	1160:1164	arg1	repair					1166:1171	skin wound repair	1155:1171	skin wound repair	1155:1171	Therefore, CLiS may be a promising therapeutic dressing for skin wound repair and regeneration.					
30701455	4	3	theme	sterile	504:510	arg1	scaffold					537:544	a sterile and biocompatible sponge scaffold	502:544	a sterile and biocompatible sponge scaffold	502:544	In this study, we loaded lithium chloride (LiCl) into the chitosan (CHI) hydrogel and develop a sterile and biocompatible sponge scaffold through freeze-drying.					
30701455	5	4	theme	CHI-LiCl	611:618	arg1	CLiS					641:644	CLiS	641:644	CLiS	641:644	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	5	4	theme	CHI-LiCl	611:618	arg1	scaffolds					630:638	the CHI-LiCl composite scaffolds	607:638	the CHI-LiCl composite scaffolds (CLiS)	607:645	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	1	5	theme	repair	189:194	arg1	mechanisms					196:205	endogenous repair mechanisms	178:205	endogenous repair mechanisms for in situ skin regeneration	178:235	Small molecules loaded into biological materials present a promising strategy for stimulating endogenous repair mechanisms for in situ skin regeneration.					
30701455	5	6	contain	possessed	647:655	arg2	biodegradation					699:712	biodegradation	699:712	biodegradation	699:712	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	5	6	contain	possessed	647:655	arg1	CLiS					641:644	CLiS	641:644	CLiS	641:644	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	5	6	contain	possessed	647:655	arg2	swelling					686:693	swelling	686:693	swelling	686:693	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	5	6	contain	possessed	647:655	arg1	scaffolds					630:638	the CHI-LiCl composite scaffolds	607:638	the CHI-LiCl composite scaffolds (CLiS)	607:645	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	5	6	contain	possessed	647:655	arg2	cytocompatibility					667:683	favorable cytocompatibility	657:683	favorable cytocompatibility	657:683	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	5	7	theme	composite	620:628	arg1	CLiS					641:644	CLiS	641:644	CLiS	641:644	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	5	7	theme	composite	620:628	arg1	scaffolds					630:638	the CHI-LiCl composite scaffolds	607:638	the CHI-LiCl composite scaffolds (CLiS)	607:645	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	7	8	theme	sustained	1005:1013	arg1	expression					1020:1029	sustained high expression	1005:1029	sustained high expression of β-catenin	1005:1042	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	8	theme	sustained	1005:1013	arg1	inflammation					934:945	reduced inflammation	926:945	reduced inflammation	926:945	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	9	dep	control	844:850	arg1	groups					874:879	groups	874:879	groups	874:879	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	4	10	theme	CHI	476:478	arg1	hydrogel					481:488	the chitosan (CHI) hydrogel	462:488	the chitosan (CHI) hydrogel	462:488	In this study, we loaded lithium chloride (LiCl) into the chitosan (CHI) hydrogel and develop a sterile and biocompatible sponge scaffold through freeze-drying.					
30701455	3	11	theme	skin	376:379	arg1	repair					381:386	skin repair	376:386	skin repair	376:386	However, its role in skin repair is rarely reported.					
30701455	1	12	theme	Small	84:88	arg1	molecules					90:98	Small molecules	84:98	Small molecules loaded into biological materials	84:131	Small molecules loaded into biological materials present a promising strategy for stimulating endogenous repair mechanisms for in situ skin regeneration.					
30701455	6	13	from	wounds	746:751	arg1	mice					769:772	male C57BL/c mice	756:772	male C57BL/c mice	756:772	We created full-thickness skin wounds in male C57BL/c mice to evaluate the healing capacity of CLiS.					
30701455	6	14	theme	skin	741:744	arg1	wounds					746:751	full-thickness skin wounds	726:751	full-thickness skin wounds in male C57BL/c mice	726:772	We created full-thickness skin wounds in male C57BL/c mice to evaluate the healing capacity of CLiS.					
30701455	0	15	theme	composite	14:22	arg1	scaffolds					24:32	Chitosan/LiCl composite scaffolds	0:32	Chitosan/LiCl composite scaffolds	0:32	Chitosan/LiCl composite scaffolds promote skin regeneration in full-thickness loss.					
30701455	7	16	theme	improved	948:955	arg1	inflammation					934:945	reduced inflammation	926:945	reduced inflammation	926:945	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	16	theme	improved	948:955	arg1	angiogenesis					957:968	improved angiogenesis	948:968	improved angiogenesis	948:968	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	6	17	theme	full-thickness	726:739	arg1	wounds					746:751	full-thickness skin wounds	726:751	full-thickness skin wounds in male C57BL/c mice	726:772	We created full-thickness skin wounds in male C57BL/c mice to evaluate the healing capacity of CLiS.					
30701455	1	18	theme	in	211:212	arg1	regeneration					224:235	in situ skin regeneration	211:235	in situ skin regeneration	211:235	Small molecules loaded into biological materials present a promising strategy for stimulating endogenous repair mechanisms for in situ skin regeneration.					
30701455	0	19	theme	Chitosan/LiCl	0:12	arg1	scaffolds					24:32	Chitosan/LiCl composite scaffolds	0:32	Chitosan/LiCl composite scaffolds	0:32	Chitosan/LiCl composite scaffolds promote skin regeneration in full-thickness loss.					
30701455	5	20	theme	favorable	657:665	arg1	cytocompatibility					667:683	favorable cytocompatibility	657:683	favorable cytocompatibility	657:683	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	6	21	theme	CLiS	810:813	arg1	capacity					798:805	the healing capacity	786:805	the healing capacity of CLiS	786:813	We created full-thickness skin wounds in male C57BL/c mice to evaluate the healing capacity of CLiS.					
30701455	7	22	theme	CHI	856:858	arg1	CS					870:871	CS	870:871	CS	870:871	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	22	theme	CHI	856:858	arg1	scaffold					860:867	CHI scaffold	856:867	CHI scaffold (CS)	856:872	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	23	theme	reduced	926:932	arg1	expression					1020:1029	sustained high expression	1005:1029	sustained high expression of β-catenin	1005:1042	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	23	theme	reduced	926:932	arg1	inflammation					934:945	reduced inflammation	926:945	reduced inflammation	926:945	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	23	theme	reduced	926:932	arg1	angiogenesis					957:968	improved angiogenesis	948:968	improved angiogenesis	948:968	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	23	theme	reduced	926:932	arg1	re-epithelialization					983:1002	accelerated re-epithelialization	971:1002	accelerated re-epithelialization	971:1002	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	1	24	theme	biological	112:121	arg1	materials					123:131	biological materials	112:131	biological materials	112:131	Small molecules loaded into biological materials present a promising strategy for stimulating endogenous repair mechanisms for in situ skin regeneration.					
30701455	1	25	dep	in	211:212	arg1	situ					214:217	situ	214:217	situ	214:217	Small molecules loaded into biological materials present a promising strategy for stimulating endogenous repair mechanisms for in situ skin regeneration.					
30701455	1	26	theme	skin	219:222	arg1	regeneration					224:235	in situ skin regeneration	211:235	in situ skin regeneration	211:235	Small molecules loaded into biological materials present a promising strategy for stimulating endogenous repair mechanisms for in situ skin regeneration.					
30701455	7	27	from	wounds	886:891	arg1	group					913:917	the CLiS-treated group	896:917	the CLiS-treated group	896:917	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	2	28	theme	decreasing	330:339	arg1	inflammation					341:352	decreasing inflammation	330:352	decreasing inflammation	330:352	Lithium can modulate various biologic processes, promoting proliferation, angiogenesis, and decreasing inflammation.					
30701455	7	29	theme	control	844:850	arg1	wounds					834:839	the wounds	830:839	the wounds of control and CHI scaffold (CS) groups	830:879	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	30	theme	hair	1079:1082	arg1	follicles					1084:1092	regenerated hair follicles	1067:1092	regenerated hair follicles	1067:1092	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	2	31	theme	biologic	267:274	arg1	processes					276:284	various biologic processes	259:284	various biologic processes	259:284	Lithium can modulate various biologic processes, promoting proliferation, angiogenesis, and decreasing inflammation.					
30701455	0	32	theme	skin	42:45	arg1	regeneration					47:58	skin regeneration	42:58	skin regeneration	42:58	Chitosan/LiCl composite scaffolds promote skin regeneration in full-thickness loss.					
30701455	4	33	theme	biocompatible	516:528	arg1	scaffold					537:544	a sterile and biocompatible sponge scaffold	502:544	a sterile and biocompatible sponge scaffold	502:544	In this study, we loaded lithium chloride (LiCl) into the chitosan (CHI) hydrogel and develop a sterile and biocompatible sponge scaffold through freeze-drying.					
30701455	7	34	theme	follicles	1084:1092	arg1	follicles					1084:1092	regenerated hair follicles	1067:1092	regenerated hair follicles	1067:1092	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	34	theme	follicles	1084:1092	arg1	amount					1057:1062	a small amount	1049:1062	a small amount of regenerated hair follicles	1049:1092	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	4	35	theme	chitosan	466:473	arg1	hydrogel					481:488	the chitosan (CHI) hydrogel	462:488	the chitosan (CHI) hydrogel	462:488	In this study, we loaded lithium chloride (LiCl) into the chitosan (CHI) hydrogel and develop a sterile and biocompatible sponge scaffold through freeze-drying.					
30701455	2	36	theme	various	259:265	arg1	processes					276:284	various biologic processes	259:284	various biologic processes	259:284	Lithium can modulate various biologic processes, promoting proliferation, angiogenesis, and decreasing inflammation.					
30701455	0	37	theme	full-thickness	63:76	arg1	loss					78:81	full-thickness loss	63:81	full-thickness loss	63:81	Chitosan/LiCl composite scaffolds promote skin regeneration in full-thickness loss.					
30701455	8	38	theme	promising	1120:1128	arg1	CLiS					1106:1109	CLiS	1106:1109	CLiS	1106:1109	Therefore, CLiS may be a promising therapeutic dressing for skin wound repair and regeneration.					
30701455	8	38	theme	promising	1120:1128	arg1	dressing					1142:1149	a promising therapeutic dressing	1118:1149	a promising therapeutic dressing for skin wound repair and regeneration	1118:1188	Therefore, CLiS may be a promising therapeutic dressing for skin wound repair and regeneration.					
30701455	6	39	theme	healing	790:796	arg1	capacity					798:805	the healing capacity	786:805	the healing capacity of CLiS	786:813	We created full-thickness skin wounds in male C57BL/c mice to evaluate the healing capacity of CLiS.					
30701455	7	40	theme	regenerated	1067:1077	arg1	follicles					1084:1092	regenerated hair follicles	1067:1092	regenerated hair follicles	1067:1092	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	4	41	theme	sponge	530:535	arg1	scaffold					537:544	a sterile and biocompatible sponge scaffold	502:544	a sterile and biocompatible sponge scaffold	502:544	In this study, we loaded lithium chloride (LiCl) into the chitosan (CHI) hydrogel and develop a sterile and biocompatible sponge scaffold through freeze-drying.					
30701455	7	42	theme	small	1051:1055	arg1	follicles					1084:1092	regenerated hair follicles	1067:1092	regenerated hair follicles	1067:1092	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	42	theme	small	1051:1055	arg1	amount					1057:1062	a small amount	1049:1062	a small amount of regenerated hair follicles	1049:1092	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	4	43	theme	lithium	433:439	arg1	LiCl					451:454	LiCl	451:454	LiCl	451:454	In this study, we loaded lithium chloride (LiCl) into the chitosan (CHI) hydrogel and develop a sterile and biocompatible sponge scaffold through freeze-drying.					
30701455	4	43	theme	lithium	433:439	arg1	chloride					441:448	lithium chloride	433:448	lithium chloride (LiCl)	433:455	In this study, we loaded lithium chloride (LiCl) into the chitosan (CHI) hydrogel and develop a sterile and biocompatible sponge scaffold through freeze-drying.					
30701455	5	44	theme	In-vitro	569:576	arg1	assessment					578:587	In-vitro assessment	569:587	In-vitro assessment	569:587	In-vitro assessment demonstrated that the CHI-LiCl composite scaffolds (CLiS) possessed favorable cytocompatibility, swelling and biodegradation.					
30701455	1	45	theme	promising	143:151	arg1	strategy					153:160	a promising strategy	141:160	a promising strategy for stimulating endogenous repair mechanisms for in situ skin regeneration	141:235	Small molecules loaded into biological materials present a promising strategy for stimulating endogenous repair mechanisms for in situ skin regeneration.					
30701455	7	46	theme	β-catenin	1034:1042	arg1	expression					1020:1029	sustained high expression	1005:1029	sustained high expression of β-catenin	1005:1042	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	46	theme	β-catenin	1034:1042	arg1	inflammation					934:945	reduced inflammation	926:945	reduced inflammation	926:945	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	3	47	from	role	368:371	arg1	repair					381:386	skin repair	376:386	skin repair	376:386	However, its role in skin repair is rarely reported.					
30701455	7	48	theme	high	1015:1018	arg1	expression					1020:1029	sustained high expression	1005:1029	sustained high expression of β-catenin	1005:1042	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	48	theme	high	1015:1018	arg1	inflammation					934:945	reduced inflammation	926:945	reduced inflammation	926:945	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	49	theme	scaffold	860:867	arg1	wounds					834:839	the wounds	830:839	the wounds of control and CHI scaffold (CS) groups	830:879	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	8	50	theme	skin	1155:1158	arg1	repair					1166:1171	skin wound repair	1155:1171	skin wound repair	1155:1171	Therefore, CLiS may be a promising therapeutic dressing for skin wound repair and regeneration.					
30701455	7	51	theme	accelerated	971:981	arg1	inflammation					934:945	reduced inflammation	926:945	reduced inflammation	926:945	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	51	theme	accelerated	971:981	arg1	re-epithelialization					983:1002	accelerated re-epithelialization	971:1002	accelerated re-epithelialization	971:1002	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	7	52	theme	CLiS-treated	900:911	arg1	group					913:917	the CLiS-treated group	896:917	the CLiS-treated group	896:917	Compared with the wounds of control and CHI scaffold (CS) groups, the wounds in the CLiS-treated group showed reduced inflammation, improved angiogenesis, accelerated re-epithelialization, sustained high expression of β-catenin with a small amount of regenerated hair follicles.					
30701455	6	53	theme	C57BL/c	761:767	arg1	mice					769:772	male C57BL/c mice	756:772	male C57BL/c mice	756:772	We created full-thickness skin wounds in male C57BL/c mice to evaluate the healing capacity of CLiS.					
30701455	8	54	theme	therapeutic	1130:1140	arg1	CLiS					1106:1109	CLiS	1106:1109	CLiS	1106:1109	Therefore, CLiS may be a promising therapeutic dressing for skin wound repair and regeneration.					
30701455	8	54	theme	therapeutic	1130:1140	arg1	dressing					1142:1149	a promising therapeutic dressing	1118:1149	a promising therapeutic dressing for skin wound repair and regeneration	1118:1188	Therefore, CLiS may be a promising therapeutic dressing for skin wound repair and regeneration.					
31132153	7	0	theme	T1	1086:1087	arg1	samples					1096:1102	T1 and T2 samples	1086:1102	T1 and T2 samples	1086:1102	However, in comparison with C, pH, color, and texture of T1 and T2 samples were affected (P < 0.05).					
31132153	4	1	theme	study	521:525	arg1	objective					503:511	The objective	499:511	The objective of this study	499:525	The objective of this study was to develop a meat sausage with pectin-encapsulated-fat (PEF) to decrease its lipid digestibility, without compromising its acceptability.					
31132153	3	2	from	product	446:452	arg1	intake					427:432	fat intake	423:432	fat intake from a meat product	423:452	Fat encapsulation with a nondigestible carbohydrate, such as pectin, may offer a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption.					
31132153	10	3	theme	lipid	1476:1480	arg1	reduction					1496:1504	lipid digestibility reduction	1476:1504	lipid digestibility reduction of T2 samples	1476:1518	Confocal images confirmed lipid digestibility reduction of T2 samples.					
31132153	13	4	theme	meat	1917:1920	arg1	products					1922:1929	meat products	1917:1929	meat products	1917:1929	PRACTICAL APPLICATION: Reduction or replacement strategies tested to modify or decrease fat content in meat products usually leads to nondesirable sensory attributes.					
31132153	2	5	theme	high	231:234	arg1	consumption					236:246	a high consumption	229:246	a high consumption of saturated fat	229:263	However, a high consumption of saturated fat has been related to several health problems.					
31132153	2	5	theme	high	231:234	arg1	related					274:280	related	274:280	related	274:280	However, a high consumption of saturated fat has been related to several health problems.					
31132153	14	6	theme	full-animal-fat	2123:2137	arg1	products					2144:2151	full-animal-fat meat products	2123:2151	full-animal-fat meat products	2123:2151	However, decreasing lipid digestibility by encapsulating animal fat with nondigestible pectin offers a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability.					
31132153	8	7	contain	had	1158:1160	arg1	changes					1150:1156	these changes	1144:1156	these changes	1144:1156	Nevertheless, these changes had no influence (P > 0.05) on sensory acceptability of treated samples, and actually improved (P < 0.05) their texture acceptance.					
31132153	8	7	contain	had	1158:1160	arg2	P > 0.05					1176:1183	P > 0.05	1176:1183	P > 0.05	1176:1183	Nevertheless, these changes had no influence (P > 0.05) on sensory acceptability of treated samples, and actually improved (P < 0.05) their texture acceptance.					
31132153	8	7	contain	had	1158:1160	arg2	influence					1165:1173	no influence	1162:1173	no influence (P > 0.05)	1162:1184	Nevertheless, these changes had no influence (P > 0.05) on sensory acceptability of treated samples, and actually improved (P < 0.05) their texture acceptance.					
31132153	14	8	from	products	2144:2151	arg1	intake					2111:2116	fat intake	2107:2116	fat intake from full-animal-fat meat products	2107:2151	However, decreasing lipid digestibility by encapsulating animal fat with nondigestible pectin offers a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability.					
31132153	11	9	theme	meat	1547:1550	arg1	sausage					1552:1558	a meat sausage	1545:1558	a meat sausage	1545:1558	Incorporation of PEF in a meat sausage offers a better protection against the hydrolytic action of lipases over triaclyglycerides, than a direct addition of pectin, without affecting its sensory acceptability.					
31132153	2	10	theme	fat	261:263	arg1	consumption					236:246	a high consumption	229:246	a high consumption of saturated fat	229:263	However, a high consumption of saturated fat has been related to several health problems.					
31132153	2	10	theme	fat	261:263	arg1	related					274:280	related	274:280	related	274:280	However, a high consumption of saturated fat has been related to several health problems.					
31132153	5	11	theme	meat	773:776	arg1	processing					778:787	meat processing	773:787	meat processing	773:787	Pork fat particles encapsulation by emulsification with a 4% pectin solution, and also stability during meat processing and cooking, was confirmed by confocal microscopy.					
31132153	9	12	theme	digestive	1299:1307	arg1	degradation					1309:1319	In vitro digestive degradation	1290:1319	In vitro digestive degradation of triacylglycerols	1290:1339	In vitro digestive degradation of triacylglycerols was decreased (P < 0.05) by 20% on T2 samples compared to control and it was superior (P < 0.05) to T1 (8%).					
31132153	8	13	theme	texture	1270:1276	arg1	acceptance					1278:1287	(P < 0.05) their texture acceptance	1253:1287	(P < 0.05) their texture acceptance	1253:1287	Nevertheless, these changes had no influence (P > 0.05) on sensory acceptability of treated samples, and actually improved (P < 0.05) their texture acceptance.					
31132153	3	14	with	encapsulation	314:326	arg1	carbohydrate					349:360	a nondigestible carbohydrate	333:360	a nondigestible carbohydrate	333:360	Fat encapsulation with a nondigestible carbohydrate, such as pectin, may offer a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption.					
31132153	3	14	with	encapsulation	314:326	arg1	pectin					371:376	pectin	371:376	pectin	371:376	Fat encapsulation with a nondigestible carbohydrate, such as pectin, may offer a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption.					
31132153	11	15	theme	better	1569:1574	arg1	protection					1576:1585	a better protection	1567:1585	a better protection against the hydrolytic action of lipases	1567:1626	Incorporation of PEF in a meat sausage offers a better protection against the hydrolytic action of lipases over triaclyglycerides, than a direct addition of pectin, without affecting its sensory acceptability.					
31132153	11	16	theme	sensory	1708:1714	arg1	acceptability					1716:1728	its sensory acceptability	1704:1728	its sensory acceptability	1704:1728	Incorporation of PEF in a meat sausage offers a better protection against the hydrolytic action of lipases over triaclyglycerides, than a direct addition of pectin, without affecting its sensory acceptability.					
31132153	14	17	theme	fat	2107:2109	arg1	intake					2111:2116	fat intake	2107:2116	fat intake from full-animal-fat meat products	2107:2151	However, decreasing lipid digestibility by encapsulating animal fat with nondigestible pectin offers a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability.					
31132153	1	18	theme	Animal	118:123	arg1	fat					125:127	Animal fat	118:127	Animal fat	118:127	Animal fat plays a key role in the structure, quality, and acceptability of emulsified meat products.					
31132153	12	19	theme	meat	1799:1802	arg1	products					1804:1811	meat products	1799:1811	meat products	1799:1811	Therefore, it can be a potential strategy to reduce fat intake from meat products.					
31132153	0	20	theme	Meat	52:55	arg1	Product					57:63	an Emulsified Meat Product	38:63	an Emulsified Meat Product	38:63	In Vitro Digestibility and Quality of an Emulsified Meat Product Formulated With Animal Fat Encapsulated With Pectin.					
31132153	13	21	theme	PRACTICAL	1814:1822	arg1	APPLICATION					1824:1834	PRACTICAL APPLICATION	1814:1834	PRACTICAL APPLICATION: Reduction or replacement strategies tested to modify or decrease fat content in meat products	1814:1929	PRACTICAL APPLICATION: Reduction or replacement strategies tested to modify or decrease fat content in meat products usually leads to nondesirable sensory attributes.					
31132153	11	22	theme	hydrolytic	1599:1608	arg1	action					1610:1615	the hydrolytic action	1595:1615	the hydrolytic action of lipases	1595:1626	Incorporation of PEF in a meat sausage offers a better protection against the hydrolytic action of lipases over triaclyglycerides, than a direct addition of pectin, without affecting its sensory acceptability.					
31132153	14	23	theme	new	2084:2086	arg1	approach					2088:2095	a new approach	2082:2095	a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability	2082:2198	However, decreasing lipid digestibility by encapsulating animal fat with nondigestible pectin offers a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability.					
31132153	6	24	theme	thermal	900:906	arg1	stability					908:916	thermal stability	900:916	thermal stability	900:916	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	0	25	theme	Animal	81:86	arg1	Fat					88:90	Animal Fat	81:90	Animal Fat Encapsulated With Pectin	81:115	In Vitro Digestibility and Quality of an Emulsified Meat Product Formulated With Animal Fat Encapsulated With Pectin.					
31132153	7	26	dep	affected	1109:1116	arg1	P < 0.05					1119:1126	P < 0.05	1119:1126	P < 0.05	1119:1126	However, in comparison with C, pH, color, and texture of T1 and T2 samples were affected (P < 0.05).					
31132153	3	27	dep	such	363:366	arg1	as					368:369	as	368:369	as	368:369	Fat encapsulation with a nondigestible carbohydrate, such as pectin, may offer a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption.					
31132153	6	28	located	found	891:895	arg2	P > 0.05					852:859	P > 0.05	852:859	P > 0.05	852:859	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	6	28	located	found	891:895	arg1	composition					922:932	composition	922:932	composition	922:932	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	6	28	located	found	891:895	arg2	changes					843:849	No changes	840:849	No changes (P > 0.05) compared to Control (C)	840:884	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	6	28	located	found	891:895	arg1	stability					908:916	thermal stability	900:916	thermal stability	900:916	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	14	29	theme	nondigestible	2054:2066	arg1	pectin					2068:2073	nondigestible pectin	2054:2073	nondigestible pectin	2054:2073	However, decreasing lipid digestibility by encapsulating animal fat with nondigestible pectin offers a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability.					
31132153	8	30	theme	sensory	1189:1195	arg1	acceptability					1197:1209	sensory acceptability	1189:1209	sensory acceptability of treated samples	1189:1228	Nevertheless, these changes had no influence (P > 0.05) on sensory acceptability of treated samples, and actually improved (P < 0.05) their texture acceptance.					
31132153	0	31	theme	In	0:1	arg1	Digestibility					9:21	In Vitro Digestibility	0:21	In Vitro Digestibility	0:21	In Vitro Digestibility and Quality of an Emulsified Meat Product Formulated With Animal Fat Encapsulated With Pectin.					
31132153	9	32	theme	T2	1376:1377	arg1	samples					1379:1385	T2 samples	1376:1385	T2 samples	1376:1385	In vitro digestive degradation of triacylglycerols was decreased (P < 0.05) by 20% on T2 samples compared to control and it was superior (P < 0.05) to T1 (8%).					
31132153	9	33	dep	decreased	1345:1353	arg1	P <					1356:1358	P <	1356:1358	P <	1356:1358	In vitro digestive degradation of triacylglycerols was decreased (P < 0.05) by 20% on T2 samples compared to control and it was superior (P < 0.05) to T1 (8%).					
31132153	1	34	dep	structure	153:161	arg1	the					149:151	the	149:151	the	149:151	Animal fat plays a key role in the structure, quality, and acceptability of emulsified meat products.					
31132153	6	35	theme	direct	962:967	arg1	addition					969:976	direct addition	962:976	direct addition of pectin (T1)	962:991	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	8	36	theme	samples	1222:1228	arg1	acceptability					1197:1209	sensory acceptability	1189:1209	sensory acceptability of treated samples	1189:1228	Nevertheless, these changes had no influence (P > 0.05) on sensory acceptability of treated samples, and actually improved (P < 0.05) their texture acceptance.					
31132153	11	37	theme	pectin	1678:1683	arg1	addition					1666:1673	a direct addition	1657:1673	a direct addition of pectin	1657:1683	Incorporation of PEF in a meat sausage offers a better protection against the hydrolytic action of lipases over triaclyglycerides, than a direct addition of pectin, without affecting its sensory acceptability.					
31132153	5	38	theme	%	728:728	arg1	solution					737:744	a 4% pectin solution	725:744	a 4% pectin solution	725:744	Pork fat particles encapsulation by emulsification with a 4% pectin solution, and also stability during meat processing and cooking, was confirmed by confocal microscopy.					
31132153	13	39	theme	nondesirable	1948:1959	arg1	attributes					1969:1978	nondesirable sensory attributes	1948:1978	nondesirable sensory attributes	1948:1978	PRACTICAL APPLICATION: Reduction or replacement strategies tested to modify or decrease fat content in meat products usually leads to nondesirable sensory attributes.					
31132153	2	40	theme	several	285:291	arg1	problems					300:307	several health problems	285:307	several health problems	285:307	However, a high consumption of saturated fat has been related to several health problems.					
31132153	10	41	theme	T2	1509:1510	arg1	samples					1512:1518	T2 samples	1509:1518	T2 samples	1509:1518	Confocal images confirmed lipid digestibility reduction of T2 samples.					
31132153	5	42	theme	confocal	819:826	arg1	microscopy					828:837	confocal microscopy	819:837	confocal microscopy	819:837	Pork fat particles encapsulation by emulsification with a 4% pectin solution, and also stability during meat processing and cooking, was confirmed by confocal microscopy.					
31132153	14	43	theme	lipid	2001:2005	arg1	digestibility					2007:2019	lipid digestibility	2001:2019	lipid digestibility	2001:2019	However, decreasing lipid digestibility by encapsulating animal fat with nondigestible pectin offers a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability.					
31132153	1	44	theme	meat	205:208	arg1	products					210:217	emulsified meat products	194:217	emulsified meat products	194:217	Animal fat plays a key role in the structure, quality, and acceptability of emulsified meat products.					
31132153	0	45	theme	Emulsified	41:50	arg1	Product					57:63	an Emulsified Meat Product	38:63	an Emulsified Meat Product	38:63	In Vitro Digestibility and Quality of an Emulsified Meat Product Formulated With Animal Fat Encapsulated With Pectin.					
31132153	0	46	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Digestibility and Quality of an Emulsified Meat Product Formulated With Animal Fat Encapsulated With Pectin.					
31132153	7	47	theme	samples	1096:1102	arg1	color					1064:1068	color	1064:1068	color	1064:1068	However, in comparison with C, pH, color, and texture of T1 and T2 samples were affected (P < 0.05).					
31132153	7	47	theme	samples	1096:1102	arg1	texture					1075:1081	texture	1075:1081	texture	1075:1081	However, in comparison with C, pH, color, and texture of T1 and T2 samples were affected (P < 0.05).					
31132153	7	47	theme	samples	1096:1102	arg1	pH					1060:1061	pH	1060:1061	pH	1060:1061	However, in comparison with C, pH, color, and texture of T1 and T2 samples were affected (P < 0.05).					
31132153	4	48	theme	meat	544:547	arg1	sausage					549:555	a meat sausage	542:555	a meat sausage with pectin-encapsulated-fat (PEF)	542:590	The objective of this study was to develop a meat sausage with pectin-encapsulated-fat (PEF) to decrease its lipid digestibility, without compromising its acceptability.					
31132153	14	49	theme	sensory	2178:2184	arg1	acceptability					2186:2198	their sensory acceptability	2172:2198	their sensory acceptability	2172:2198	However, decreasing lipid digestibility by encapsulating animal fat with nondigestible pectin offers a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability.					
31132153	10	50	theme	digestibility	1482:1494	arg1	reduction					1496:1504	lipid digestibility reduction	1476:1504	lipid digestibility reduction of T2 samples	1476:1518	Confocal images confirmed lipid digestibility reduction of T2 samples.					
31132153	13	51	theme	fat	1902:1904	arg1	content					1906:1912	fat content	1902:1912	fat content in meat products	1902:1929	PRACTICAL APPLICATION: Reduction or replacement strategies tested to modify or decrease fat content in meat products usually leads to nondesirable sensory attributes.					
31132153	12	52	theme	fat	1783:1785	arg1	intake					1787:1792	fat intake	1783:1792	fat intake from meat products	1783:1811	Therefore, it can be a potential strategy to reduce fat intake from meat products.					
31132153	2	53	theme	saturated	251:259	arg1	fat					261:263	saturated fat	251:263	saturated fat	251:263	However, a high consumption of saturated fat has been related to several health problems.					
31132153	10	54	theme	samples	1512:1518	arg1	reduction					1496:1504	lipid digestibility reduction	1476:1504	lipid digestibility reduction of T2 samples	1476:1518	Confocal images confirmed lipid digestibility reduction of T2 samples.					
31132153	12	55	theme	potential	1754:1762	arg1	strategy					1764:1771	a potential strategy	1752:1771	a potential strategy to reduce fat intake from meat products	1752:1811	Therefore, it can be a potential strategy to reduce fat intake from meat products.					
31132153	12	55	theme	potential	1754:1762	arg1	it					1742:1743	it	1742:1743	it	1742:1743	Therefore, it can be a potential strategy to reduce fat intake from meat products.					
31132153	14	56	theme	meat	2139:2142	arg1	products					2144:2151	full-animal-fat meat products	2123:2151	full-animal-fat meat products	2123:2151	However, decreasing lipid digestibility by encapsulating animal fat with nondigestible pectin offers a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability.					
31132153	7	57	with	comparison	1041:1050	arg1	C					1057:1057	C	1057:1057	C	1057:1057	However, in comparison with C, pH, color, and texture of T1 and T2 samples were affected (P < 0.05).					
31132153	3	58	theme	meat	441:444	arg1	product					446:452	a meat product	439:452	a meat product	439:452	Fat encapsulation with a nondigestible carbohydrate, such as pectin, may offer a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption.					
31132153	4	59	with	sausage	549:555	arg1	PEF					587:589	PEF	587:589	PEF	587:589	The objective of this study was to develop a meat sausage with pectin-encapsulated-fat (PEF) to decrease its lipid digestibility, without compromising its acceptability.					
31132153	4	59	with	sausage	549:555	arg1	pectin-encapsulated-fat					562:584	pectin-encapsulated-fat	562:584	pectin-encapsulated-fat (PEF)	562:590	The objective of this study was to develop a meat sausage with pectin-encapsulated-fat (PEF) to decrease its lipid digestibility, without compromising its acceptability.					
31132153	5	60	with	encapsulation	688:700	arg1	solution					737:744	a 4% pectin solution	725:744	a 4% pectin solution	725:744	Pork fat particles encapsulation by emulsification with a 4% pectin solution, and also stability during meat processing and cooking, was confirmed by confocal microscopy.					
31132153	9	61	dep	superior	1418:1425	arg1	%					1446:1446	8%	1445:1446	8%	1445:1446	In vitro digestive degradation of triacylglycerols was decreased (P < 0.05) by 20% on T2 samples compared to control and it was superior (P < 0.05) to T1 (8%).					
31132153	9	61	dep	superior	1418:1425	arg1	P <					1428:1430	P < 0.05	1428:1435	P < 0.05	1428:1435	In vitro digestive degradation of triacylglycerols was decreased (P < 0.05) by 20% on T2 samples compared to control and it was superior (P < 0.05) to T1 (8%).					
31132153	13	62	theme	replacement	1850:1860	arg1	strategies					1862:1871	Reduction or replacement strategies	1837:1871	strategies	1862:1871	PRACTICAL APPLICATION: Reduction or replacement strategies tested to modify or decrease fat content in meat products usually leads to nondesirable sensory attributes.					
31132153	9	63	theme	triacylglycerols	1324:1339	arg1	degradation					1309:1319	In vitro digestive degradation	1290:1319	In vitro digestive degradation of triacylglycerols	1290:1339	In vitro digestive degradation of triacylglycerols was decreased (P < 0.05) by 20% on T2 samples compared to control and it was superior (P < 0.05) to T1 (8%).					
31132153	6	64	theme	sausages	937:944	arg1	composition					922:932	composition	922:932	composition	922:932	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	6	64	theme	sausages	937:944	arg1	stability					908:916	thermal stability	900:916	thermal stability	900:916	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	11	65	from	Incorporation	1521:1533	arg1	sausage					1552:1558	a meat sausage	1545:1558	a meat sausage	1545:1558	Incorporation of PEF in a meat sausage offers a better protection against the hydrolytic action of lipases over triaclyglycerides, than a direct addition of pectin, without affecting its sensory acceptability.					
31132153	10	66	theme	Confocal	1450:1457	arg1	images					1459:1464	Confocal images	1450:1464	Confocal images	1450:1464	Confocal images confirmed lipid digestibility reduction of T2 samples.					
31132153	0	67	theme	Product	57:63	arg1	Digestibility					9:21	In Vitro Digestibility	0:21	In Vitro Digestibility	0:21	In Vitro Digestibility and Quality of an Emulsified Meat Product Formulated With Animal Fat Encapsulated With Pectin.					
31132153	0	67	theme	Product	57:63	arg1	Quality					27:33	Quality	27:33	Quality	27:33	In Vitro Digestibility and Quality of an Emulsified Meat Product Formulated With Animal Fat Encapsulated With Pectin.					
31132153	2	68	theme	health	293:298	arg1	problems					300:307	several health problems	285:307	several health problems	285:307	However, a high consumption of saturated fat has been related to several health problems.					
31132153	12	69	from	products	1804:1811	arg1	intake					1787:1792	fat intake	1783:1792	fat intake from meat products	1783:1811	Therefore, it can be a potential strategy to reduce fat intake from meat products.					
31132153	4	70	theme	lipid	608:612	arg1	digestibility					614:626	its lipid digestibility	604:626	its lipid digestibility	604:626	The objective of this study was to develop a meat sausage with pectin-encapsulated-fat (PEF) to decrease its lipid digestibility, without compromising its acceptability.					
31132153	5	71	theme	fat	674:676	arg1	encapsulation					688:700	Pork fat particles encapsulation	669:700	Pork fat particles encapsulation by emulsification with a 4% pectin solution	669:744	Pork fat particles encapsulation by emulsification with a 4% pectin solution, and also stability during meat processing and cooking, was confirmed by confocal microscopy.					
31132153	1	72	theme	key	137:139	arg1	role					141:144	a key role	135:144	a key role	135:144	Animal fat plays a key role in the structure, quality, and acceptability of emulsified meat products.					
31132153	9	73	from	%	1371:1371	arg1	samples					1379:1385	T2 samples	1376:1385	T2 samples	1376:1385	In vitro digestive degradation of triacylglycerols was decreased (P < 0.05) by 20% on T2 samples compared to control and it was superior (P < 0.05) to T1 (8%).					
31132153	5	74	theme	Pork	669:672	arg1	encapsulation					688:700	Pork fat particles encapsulation	669:700	Pork fat particles encapsulation by emulsification with a 4% pectin solution	669:744	Pork fat particles encapsulation by emulsification with a 4% pectin solution, and also stability during meat processing and cooking, was confirmed by confocal microscopy.					
31132153	13	75	theme	Reduction	1837:1845	arg1	strategies					1862:1871	Reduction or replacement strategies	1837:1871	strategies	1862:1871	PRACTICAL APPLICATION: Reduction or replacement strategies tested to modify or decrease fat content in meat products usually leads to nondesirable sensory attributes.					
31132153	11	76	theme	PEF	1538:1540	arg1	Incorporation					1521:1533	Incorporation	1521:1533	Incorporation of PEF in a meat sausage	1521:1558	Incorporation of PEF in a meat sausage offers a better protection against the hydrolytic action of lipases over triaclyglycerides, than a direct addition of pectin, without affecting its sensory acceptability.					
31132153	5	77	theme	particles	678:686	arg1	encapsulation					688:700	Pork fat particles encapsulation	669:700	Pork fat particles encapsulation by emulsification with a 4% pectin solution	669:744	Pork fat particles encapsulation by emulsification with a 4% pectin solution, and also stability during meat processing and cooking, was confirmed by confocal microscopy.					
31132153	9	78	theme	In	1290:1291	arg1	degradation					1309:1319	In vitro digestive degradation	1290:1319	In vitro digestive degradation of triacylglycerols	1290:1339	In vitro digestive degradation of triacylglycerols was decreased (P < 0.05) by 20% on T2 samples compared to control and it was superior (P < 0.05) to T1 (8%).					
31132153	3	79	theme	Fat	310:312	arg1	encapsulation					314:326	Fat encapsulation	310:326	Fat encapsulation with a nondigestible carbohydrate, such as pectin,	310:377	Fat encapsulation with a nondigestible carbohydrate, such as pectin, may offer a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption.					
31132153	3	80	theme	promising	391:399	arg1	alternative					401:411	a promising alternative	389:411	a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption	389:496	Fat encapsulation with a nondigestible carbohydrate, such as pectin, may offer a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption.					
31132153	6	81	theme	pectin	981:986	arg1	addition					969:976	direct addition	962:976	direct addition of pectin (T1)	962:991	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	14	82	theme	animal	2038:2043	arg1	fat					2045:2047	animal fat	2038:2047	animal fat	2038:2047	However, decreasing lipid digestibility by encapsulating animal fat with nondigestible pectin offers a new approach to reduce fat intake from full-animal-fat meat products, without affecting their sensory acceptability.					
31132153	11	83	theme	direct	1659:1664	arg1	addition					1666:1673	a direct addition	1657:1673	a direct addition of pectin	1657:1683	Incorporation of PEF in a meat sausage offers a better protection against the hydrolytic action of lipases over triaclyglycerides, than a direct addition of pectin, without affecting its sensory acceptability.					
31132153	3	84	theme	nondigestible	335:347	arg1	carbohydrate					349:360	a nondigestible carbohydrate	333:360	a nondigestible carbohydrate	333:360	Fat encapsulation with a nondigestible carbohydrate, such as pectin, may offer a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption.					
31132153	3	85	theme	fat	423:425	arg1	intake					427:432	fat intake	423:432	fat intake from a meat product	423:452	Fat encapsulation with a nondigestible carbohydrate, such as pectin, may offer a promising alternative to reduce fat intake from a meat product, by preventing its digestion and absorption.					
31132153	5	86	theme	4	727:727	arg1	%					728:728	%	728:728	%	728:728	Pork fat particles encapsulation by emulsification with a 4% pectin solution, and also stability during meat processing and cooking, was confirmed by confocal microscopy.					
31132153	13	87	from	content	1906:1912	arg1	products					1922:1929	meat products	1917:1929	meat products	1917:1929	PRACTICAL APPLICATION: Reduction or replacement strategies tested to modify or decrease fat content in meat products usually leads to nondesirable sensory attributes.					
31132153	8	88	theme	treated	1214:1220	arg1	samples					1222:1228	treated samples	1214:1228	treated samples	1214:1228	Nevertheless, these changes had no influence (P > 0.05) on sensory acceptability of treated samples, and actually improved (P < 0.05) their texture acceptance.					
31132153	5	89	theme	pectin	730:735	arg1	solution					737:744	a 4% pectin solution	725:744	a 4% pectin solution	725:744	Pork fat particles encapsulation by emulsification with a 4% pectin solution, and also stability during meat processing and cooking, was confirmed by confocal microscopy.					
31132153	13	90	theme	sensory	1961:1967	arg1	attributes					1969:1978	nondesirable sensory attributes	1948:1978	nondesirable sensory attributes	1948:1978	PRACTICAL APPLICATION: Reduction or replacement strategies tested to modify or decrease fat content in meat products usually leads to nondesirable sensory attributes.					
31132153	11	91	theme	lipases	1620:1626	arg1	action					1610:1615	the hydrolytic action	1595:1615	the hydrolytic action of lipases	1595:1626	Incorporation of PEF in a meat sausage offers a better protection against the hydrolytic action of lipases over triaclyglycerides, than a direct addition of pectin, without affecting its sensory acceptability.					
31132153	6	92	theme	PEF	1019:1021	arg1	incorporation					1002:1014	incorporation	1002:1014	incorporation of PEF (T2)	1002:1026	No changes (P > 0.05) compared to Control (C) were found on thermal stability and composition of sausages formulated with direct addition of pectin (T1) and with incorporation of PEF (T2).					
31132153	1	93	theme	emulsified	194:203	arg1	products					210:217	emulsified meat products	194:217	emulsified meat products	194:217	Animal fat plays a key role in the structure, quality, and acceptability of emulsified meat products.					
31132153	7	94	theme	T2	1093:1094	arg1	samples					1096:1102	T1 and T2 samples	1086:1102	T1 and T2 samples	1086:1102	However, in comparison with C, pH, color, and texture of T1 and T2 samples were affected (P < 0.05).					
31132153	9	95	dep	In	1290:1291	arg1	vitro					1293:1297	vitro	1293:1297	vitro	1293:1297	In vitro digestive degradation of triacylglycerols was decreased (P < 0.05) by 20% on T2 samples compared to control and it was superior (P < 0.05) to T1 (8%).					
31132153	1	96	theme	products	210:217	arg1	structure					153:161	structure	153:161	structure	153:161	Animal fat plays a key role in the structure, quality, and acceptability of emulsified meat products.					
31132153	1	96	theme	products	210:217	arg1	quality					164:170	quality	164:170	quality	164:170	Animal fat plays a key role in the structure, quality, and acceptability of emulsified meat products.					
31132153	1	96	theme	products	210:217	arg1	acceptability					177:189	acceptability	177:189	acceptability	177:189	Animal fat plays a key role in the structure, quality, and acceptability of emulsified meat products.					
31029303	0	0	theme	electrochemical	90:104	arg1	detection					106:114	electrochemical detection	90:114	electrochemical detection of clopidogrel in-vitro	90:138	Silver nanoparticles impregnated chitosan layered carbon nanotube as sensor interface for electrochemical detection of clopidogrel in-vitro.					
31029303	8	1	theme	%	1661:1661	arg1	limits					1638:1643	good recovery limits	1624:1643	good recovery limits of 95.2 to 102.6%	1624:1661	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	5	2	theme	electrode	960:968	arg1	responses					907:915	Electrochemical responses	891:915	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP	891:997	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP have been examined by cyclic voltammetry and electrochemical impedance spectroscopy.					
31029303	5	3	theme	cyclic	1021:1026	arg1	voltammetry					1028:1038	cyclic voltammetry	1021:1038	cyclic voltammetry	1021:1038	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP have been examined by cyclic voltammetry and electrochemical impedance spectroscopy.					
31029303	1	4	theme	diseases	293:300	arg1	diagnosis					246:254	medical diagnosis	238:254	medical diagnosis of heart ailments and cardiovascular diseases	238:300	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	5	5	theme	electrochemical	1044:1058	arg1	spectroscopy					1070:1081	electrochemical impedance spectroscopy	1044:1081	electrochemical impedance spectroscopy	1044:1081	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP have been examined by cyclic voltammetry and electrochemical impedance spectroscopy.					
31029303	1	6	theme	physiological	192:204	arg1	fluids					211:216	physiological body fluids	192:216	physiological body fluids	192:216	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	8	7	from	determination	1525:1537	arg1	urine					1564:1568	artificially prepared urine	1542:1568	artificially prepared urine	1542:1568	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	8	7	from	determination	1525:1537	arg1	formulation					1589:1599	pharmaceutical formulation	1574:1599	pharmaceutical formulation	1574:1599	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	2	8	theme	embedded	391:398	arg1	nanoparticles					377:389	silver nanoparticles	370:389	silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT)	370:453	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	7	9	theme	pulse	1289:1293	arg1	voltammetry					1295:1305	differential pulse voltammetry	1276:1305	differential pulse voltammetry (DPV)	1276:1311	Electrochemical determination of CLP was investigated by differential pulse voltammetry (DPV) and amperometric analysis under optimized conditions.					
31029303	7	9	theme	pulse	1289:1293	arg1	DPV					1308:1310	DPV	1308:1310	DPV	1308:1310	Electrochemical determination of CLP was investigated by differential pulse voltammetry (DPV) and amperometric analysis under optimized conditions.					
31029303	8	10	theme	pharmaceutical	1574:1587	arg1	formulation					1589:1599	pharmaceutical formulation	1574:1599	pharmaceutical formulation	1574:1599	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	3	11	theme	Raman	616:620	arg1	spectroscopy					622:633	Raman spectroscopy	616:633	Raman spectroscopy	616:633	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	3	12	theme	field	639:643	arg1	microscopy					672:681	field emission scanning electron microscopy	639:681	field emission scanning electron microscopy	639:681	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	2	13	dep	embedded	391:398	arg1	composite					432:440	chitosan-carbon nanotube hybrid composite	400:440	chitosan-carbon nanotube hybrid composite (AgChit-CNT)	400:453	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	13	dep	embedded	391:398	arg1	AgChit-CNT					443:452	AgChit-CNT	443:452	AgChit-CNT	443:452	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	5	14	theme	fabricated	924:933	arg1	electrode					960:968	the fabricated AgChit-CNT nanocomposite electrode	920:968	the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP	920:997	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP have been examined by cyclic voltammetry and electrochemical impedance spectroscopy.					
31029303	7	15	theme	amperometric	1317:1328	arg1	analysis					1330:1337	amperometric analysis	1317:1337	amperometric analysis	1317:1337	Electrochemical determination of CLP was investigated by differential pulse voltammetry (DPV) and amperometric analysis under optimized conditions.					
31029303	3	16	theme	Synthesized	545:555	arg1	nanocomposite					568:580	Synthesized AgChit-CNT nanocomposite	545:580	Synthesized AgChit-CNT nanocomposite	545:580	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	0	17	theme	in-vitro	131:138	arg1	clopidogrel					119:129	clopidogrel	119:129	clopidogrel in-vitro	119:138	Silver nanoparticles impregnated chitosan layered carbon nanotube as sensor interface for electrochemical detection of clopidogrel in-vitro.					
31029303	3	18	theme	AgChit-CNT	557:566	arg1	nanocomposite					568:580	Synthesized AgChit-CNT nanocomposite	545:580	Synthesized AgChit-CNT nanocomposite	545:580	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	7	19	theme	optimized	1345:1353	arg1	conditions					1355:1364	optimized conditions	1345:1364	optimized conditions	1345:1364	Electrochemical determination of CLP was investigated by differential pulse voltammetry (DPV) and amperometric analysis under optimized conditions.					
31029303	4	20	theme	chain	845:849	arg1	linkages					856:863	continuous chain like linkages	834:863	continuous chain like linkages with CNTs	834:873	Crystalline silver nanoparticles of about 35 nm are well distributed in the composite and have formed continuous chain like linkages with CNTs all throughout.					
31029303	2	21	theme	electrochemical	322:336	arg1	sensor					338:343	A highly sensitive electrochemical sensor	303:343	A highly sensitive electrochemical sensor	303:343	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	21	theme	electrochemical	322:336	arg1	interface					465:473	sensor interface	458:473	sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP)	458:542	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	5	22	theme	impedance	1060:1068	arg1	spectroscopy					1070:1081	electrochemical impedance spectroscopy	1044:1081	electrochemical impedance spectroscopy	1044:1081	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP have been examined by cyclic voltammetry and electrochemical impedance spectroscopy.					
31029303	1	23	theme	body	206:209	arg1	fluids					211:216	physiological body fluids	192:216	physiological body fluids	192:216	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	0	24	theme	clopidogrel	119:129	arg1	detection					106:114	electrochemical detection	90:114	electrochemical detection of clopidogrel in-vitro	90:138	Silver nanoparticles impregnated chitosan layered carbon nanotube as sensor interface for electrochemical detection of clopidogrel in-vitro.					
31029303	6	25	theme	electron	1154:1161	arg1	mediator					1172:1179	an excellent electron transfer mediator	1141:1179	an excellent electron transfer mediator towards the oxidation of clopidogrel	1141:1216	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	6	25	theme	electron	1154:1161	arg1	interface					1123:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	2	26	theme	sensitive	312:320	arg1	sensor					338:343	A highly sensitive electrochemical sensor	303:343	A highly sensitive electrochemical sensor	303:343	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	26	theme	sensitive	312:320	arg1	interface					465:473	sensor interface	458:473	sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP)	458:542	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	27	theme	sensor	458:463	arg1	sensor					338:343	A highly sensitive electrochemical sensor	303:343	A highly sensitive electrochemical sensor	303:343	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	27	theme	sensor	458:463	arg1	interface					465:473	sensor interface	458:473	sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP)	458:542	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	6	28	theme	CNT	1105:1107	arg1	mediator					1172:1179	an excellent electron transfer mediator	1141:1179	an excellent electron transfer mediator towards the oxidation of clopidogrel	1141:1216	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	6	28	theme	CNT	1105:1107	arg1	interface					1123:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	1	29	from	monitoring	163:172	arg1	fluids					211:216	physiological body fluids	192:216	physiological body fluids	192:216	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	2	30	theme	silver	370:375	arg1	nanoparticles					377:389	silver nanoparticles	370:389	silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT)	370:453	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	31	theme	drug	520:523	arg1	detection					479:487	detection	479:487	detection of the important anti-platelet drug, clopidogrel (CLP)	479:542	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	8	32	dep	102.6	1656:1660	arg1	to					1653:1654	to	1653:1654	to	1653:1654	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	3	33	theme	scanning	654:661	arg1	microscopy					672:681	field emission scanning electron microscopy	639:681	field emission scanning electron microscopy	639:681	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	6	34	theme	clopidogrel	1206:1216	arg1	oxidation					1193:1201	the oxidation	1189:1201	the oxidation of clopidogrel	1189:1216	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	4	35	theme	35 nm	774:778	arg1	nanoparticles					751:763	Crystalline silver nanoparticles	732:763	Crystalline silver nanoparticles of about 35 nm	732:778	Crystalline silver nanoparticles of about 35 nm are well distributed in the composite and have formed continuous chain like linkages with CNTs all throughout.					
31029303	0	36	theme	chitosan	33:40	arg1	nanotube					57:64	impregnated chitosan layered carbon nanotube	21:64	impregnated chitosan layered carbon nanotube	21:64	Silver nanoparticles impregnated chitosan layered carbon nanotube as sensor interface for electrochemical detection of clopidogrel in-vitro.					
31029303	4	37	theme	continuous	834:843	arg1	linkages					856:863	continuous chain like linkages	834:863	continuous chain like linkages with CNTs	834:873	Crystalline silver nanoparticles of about 35 nm are well distributed in the composite and have formed continuous chain like linkages with CNTs all throughout.					
31029303	8	38	theme	detection	1380:1388	arg1	limit					1371:1375	The limit	1367:1375	The limit of detection by DPV and amperometry	1367:1411	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	8	38	theme	detection	1380:1388	arg1	30 nM					1418:1422	30 nM	1418:1422	30 nM	1418:1422	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	6	39	theme	transfer	1163:1170	arg1	mediator					1172:1179	an excellent electron transfer mediator	1141:1179	an excellent electron transfer mediator towards the oxidation of clopidogrel	1141:1216	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	6	39	theme	transfer	1163:1170	arg1	interface					1123:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	7	40	theme	differential	1276:1287	arg1	voltammetry					1295:1305	differential pulse voltammetry	1276:1305	differential pulse voltammetry (DPV)	1276:1311	Electrochemical determination of CLP was investigated by differential pulse voltammetry (DPV) and amperometric analysis under optimized conditions.					
31029303	7	40	theme	differential	1276:1287	arg1	DPV					1308:1310	DPV	1308:1310	DPV	1308:1310	Electrochemical determination of CLP was investigated by differential pulse voltammetry (DPV) and amperometric analysis under optimized conditions.					
31029303	4	41	theme	Crystalline	732:742	arg1	nanoparticles					751:763	Crystalline silver nanoparticles	732:763	Crystalline silver nanoparticles of about 35 nm	732:778	Crystalline silver nanoparticles of about 35 nm are well distributed in the composite and have formed continuous chain like linkages with CNTs all throughout.					
31029303	0	42	theme	impregnated	21:31	arg1	nanotube					57:64	impregnated chitosan layered carbon nanotube	21:64	impregnated chitosan layered carbon nanotube	21:64	Silver nanoparticles impregnated chitosan layered carbon nanotube as sensor interface for electrochemical detection of clopidogrel in-vitro.					
31029303	6	43	theme	nanocomposite	1109:1121	arg1	mediator					1172:1179	an excellent electron transfer mediator	1141:1179	an excellent electron transfer mediator towards the oxidation of clopidogrel	1141:1216	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	6	43	theme	nanocomposite	1109:1121	arg1	interface					1123:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	7	44	theme	CLP	1252:1254	arg1	determination					1235:1247	Electrochemical determination	1219:1247	Electrochemical determination of CLP	1219:1254	Electrochemical determination of CLP was investigated by differential pulse voltammetry (DPV) and amperometric analysis under optimized conditions.					
31029303	8	45	theme	good	1624:1627	arg1	limits					1638:1643	good recovery limits	1624:1643	good recovery limits of 95.2 to 102.6%	1624:1661	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	2	46	theme	hybrid	425:430	arg1	composite					432:440	chitosan-carbon nanotube hybrid composite	400:440	chitosan-carbon nanotube hybrid composite (AgChit-CNT)	400:453	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	46	theme	hybrid	425:430	arg1	AgChit-CNT					443:452	AgChit-CNT	443:452	AgChit-CNT	443:452	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	1	47	theme	medical	238:244	arg1	diagnosis					246:254	medical diagnosis	238:254	medical diagnosis of heart ailments and cardiovascular diseases	238:300	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	0	48	theme	carbon	50:55	arg1	nanotube					57:64	impregnated chitosan layered carbon nanotube	21:64	impregnated chitosan layered carbon nanotube	21:64	Silver nanoparticles impregnated chitosan layered carbon nanotube as sensor interface for electrochemical detection of clopidogrel in-vitro.					
31029303	2	49	theme	chitosan-carbon	400:414	arg1	composite					432:440	chitosan-carbon nanotube hybrid composite	400:440	chitosan-carbon nanotube hybrid composite (AgChit-CNT)	400:453	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	49	theme	chitosan-carbon	400:414	arg1	AgChit-CNT					443:452	AgChit-CNT	443:452	AgChit-CNT	443:452	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	7	50	theme	Electrochemical	1219:1233	arg1	determination					1235:1247	Electrochemical determination	1219:1247	Electrochemical determination of CLP	1219:1254	Electrochemical determination of CLP was investigated by differential pulse voltammetry (DPV) and amperometric analysis under optimized conditions.					
31029303	0	51	theme	layered	42:48	arg1	nanotube					57:64	impregnated chitosan layered carbon nanotube	21:64	impregnated chitosan layered carbon nanotube	21:64	Silver nanoparticles impregnated chitosan layered carbon nanotube as sensor interface for electrochemical detection of clopidogrel in-vitro.					
31029303	4	52	with	linkages	856:863	arg1	CNTs					870:873	CNTs	870:873	CNTs	870:873	Crystalline silver nanoparticles of about 35 nm are well distributed in the composite and have formed continuous chain like linkages with CNTs all throughout.					
31029303	5	53	theme	Electrochemical	891:905	arg1	responses					907:915	Electrochemical responses	891:915	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP	891:997	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP have been examined by cyclic voltammetry and electrochemical impedance spectroscopy.					
31029303	6	54	theme	excellent	1144:1152	arg1	mediator					1172:1179	an excellent electron transfer mediator	1141:1179	an excellent electron transfer mediator towards the oxidation of clopidogrel	1141:1216	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	6	54	theme	excellent	1144:1152	arg1	interface					1123:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	2	55	theme	anti-platelet	506:518	arg1	drug					520:523	the important anti-platelet drug	492:523	the important anti-platelet drug	492:523	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	55	theme	anti-platelet	506:518	arg1	clopidogrel					526:536	clopidogrel	526:536	clopidogrel (CLP)	526:542	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	1	56	from	diagnosis	246:254	arg1	indispensable					221:233	indispensable	221:233	indispensable	221:233	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	4	57	theme	silver	744:749	arg1	nanoparticles					751:763	Crystalline silver nanoparticles	732:763	Crystalline silver nanoparticles of about 35 nm	732:778	Crystalline silver nanoparticles of about 35 nm are well distributed in the composite and have formed continuous chain like linkages with CNTs all throughout.					
31029303	6	58	theme	nanoAg	1088:1093	arg1	mediator					1172:1179	an excellent electron transfer mediator	1141:1179	an excellent electron transfer mediator towards the oxidation of clopidogrel	1141:1216	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	6	58	theme	nanoAg	1088:1093	arg1	interface					1123:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	8	59	theme	10 s.	1491:1495	arg1	applicability					1507:1519	10 s. Practical applicability	1491:1519	10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation	1491:1599	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	8	60	theme	recovery	1629:1636	arg1	limits					1638:1643	good recovery limits	1624:1643	good recovery limits of 95.2 to 102.6%	1624:1661	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	3	61	theme	electron	663:670	arg1	microscopy					672:681	field emission scanning electron microscopy	639:681	field emission scanning electron microscopy	639:681	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	2	62	theme	nanotube	416:423	arg1	composite					432:440	chitosan-carbon nanotube hybrid composite	400:440	chitosan-carbon nanotube hybrid composite (AgChit-CNT)	400:453	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	62	theme	nanotube	416:423	arg1	AgChit-CNT					443:452	AgChit-CNT	443:452	AgChit-CNT	443:452	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	5	63	theme	AgChit-CNT	935:944	arg1	electrode					960:968	the fabricated AgChit-CNT nanocomposite electrode	920:968	the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP	920:997	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP have been examined by cyclic voltammetry and electrochemical impedance spectroscopy.					
31029303	1	64	theme	Continuous	141:150	arg1	monitoring					163:172	Continuous periodical monitoring	141:172	Continuous periodical monitoring of clopidogrel in physiological body fluids	141:216	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	3	65	theme	emission	645:652	arg1	microscopy					672:681	field emission scanning electron microscopy	639:681	field emission scanning electron microscopy	639:681	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	6	66	theme	patterned	1095:1103	arg1	mediator					1172:1179	an excellent electron transfer mediator	1141:1179	an excellent electron transfer mediator towards the oxidation of clopidogrel	1141:1216	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	6	66	theme	patterned	1095:1103	arg1	interface					1123:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface	1084:1131	The nanoAg patterned CNT nanocomposite interface acts as an excellent electron transfer mediator towards the oxidation of clopidogrel.					
31029303	3	67	theme	structural	704:713	arg1	characteristics					715:729	its chemical and structural characteristics	687:729	its chemical and structural characteristics	687:729	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	8	68	theme	prepared	1555:1562	arg1	urine					1564:1568	artificially prepared urine	1542:1568	artificially prepared urine	1542:1568	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	1	69	theme	periodical	152:161	arg1	monitoring					163:172	Continuous periodical monitoring	141:172	Continuous periodical monitoring of clopidogrel in physiological body fluids	141:216	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	1	70	theme	heart	259:263	arg1	ailments					265:272	heart ailments	259:272	heart ailments	259:272	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	8	71	theme	Practical	1497:1505	arg1	applicability					1507:1519	10 s. Practical applicability	1491:1519	10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation	1491:1599	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	5	72	theme	nanocomposite	946:958	arg1	electrode					960:968	the fabricated AgChit-CNT nanocomposite electrode	920:968	the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP	920:997	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP have been examined by cyclic voltammetry and electrochemical impedance spectroscopy.					
31029303	4	73	theme	like	851:854	arg1	linkages					856:863	continuous chain like linkages	834:863	continuous chain like linkages with CNTs	834:873	Crystalline silver nanoparticles of about 35 nm are well distributed in the composite and have formed continuous chain like linkages with CNTs all throughout.					
31029303	3	74	theme	x-ray	597:601	arg1	diffraction					603:613	x-ray diffraction	597:613	x-ray diffraction	597:613	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	8	75	theme	analysis	1469:1476	arg1	time					1457:1460	the time	1453:1460	the time of the analysis	1453:1476	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	8	75	theme	analysis	1469:1476	arg1	low					1484:1486	low	1484:1486	low	1484:1486	The limit of detection by DPV and amperometry were 30 nM and 10 nM, respectively, and the time of the analysis is as low as 10 s. Practical applicability for determination in artificially prepared urine and pharmaceutical formulation has been examined with good recovery limits of 95.2 to 102.6%.					
31029303	1	76	theme	ailments	265:272	arg1	diagnosis					246:254	medical diagnosis	238:254	medical diagnosis of heart ailments and cardiovascular diseases	238:300	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	3	77	theme	chemical	691:698	arg1	characteristics					715:729	its chemical and structural characteristics	687:729	its chemical and structural characteristics	687:729	Synthesized AgChit-CNT nanocomposite is examined by x-ray diffraction, Raman spectroscopy and field emission scanning electron microscopy for its chemical and structural characteristics.					
31029303	1	78	from	indispensable	221:233	arg1	diagnosis					246:254	medical diagnosis	238:254	medical diagnosis of heart ailments and cardiovascular diseases	238:300	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	5	79	theme	CLP	995:997	arg1	determination					978:990	the determination	974:990	the determination of CLP	974:997	Electrochemical responses of the fabricated AgChit-CNT nanocomposite electrode for the determination of CLP have been examined by cyclic voltammetry and electrochemical impedance spectroscopy.					
31029303	0	80	theme	sensor	69:74	arg1	interface					76:84	sensor interface	69:84	sensor interface for electrochemical detection of clopidogrel in-vitro	69:138	Silver nanoparticles impregnated chitosan layered carbon nanotube as sensor interface for electrochemical detection of clopidogrel in-vitro.					
31029303	2	81	theme	important	496:504	arg1	drug					520:523	the important anti-platelet drug	492:523	the important anti-platelet drug	492:523	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	2	81	theme	important	496:504	arg1	clopidogrel					526:536	clopidogrel	526:536	clopidogrel (CLP)	526:542	A highly sensitive electrochemical sensor has been fabricated with silver nanoparticles embedded chitosan-carbon nanotube hybrid composite (AgChit-CNT) as sensor interface for detection of the important anti-platelet drug, clopidogrel (CLP).					
31029303	1	82	theme	clopidogrel	177:187	arg1	monitoring					163:172	Continuous periodical monitoring	141:172	Continuous periodical monitoring of clopidogrel in physiological body fluids	141:216	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
31029303	1	83	theme	cardiovascular	278:291	arg1	diseases					293:300	cardiovascular diseases	278:300	cardiovascular diseases	278:300	Continuous periodical monitoring of clopidogrel in physiological body fluids is indispensable in medical diagnosis of heart ailments and cardiovascular diseases.					
30690438	2	0	theme	protein	567:573	arg1	release					575:581	protein release	567:581	protein release	567:581	The physical properties, bioactivity, biological properties and their capabilities for sustained drug and protein release were studied.					
30690438	6	1	theme	@	1061:1061	arg1	case					1082:1085	the mHA@Chi-g-PMMA scaffold case	1054:1085	the mHA@Chi-g-PMMA scaffold case	1054:1085	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	3	2	theme	mineral	663:669	arg1	ions					671:674	the mineral ions	659:674	the mineral ions	659:674	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	1	3	theme	mHA	406:408	arg1	Chi-g-PMMA					410:419	mHA@Chi-g-PMMA	406:419	mHA@Chi-g-PMMA	406:419	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	3	theme	mHA	406:408	arg1	solution					396:403	a simulated body fluid (SBF) solution	367:403	a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	367:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	4	theme	mineral	260:266	arg1	mHA					296:298	mHA	296:298	mHA	296:298	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	4	theme	mineral	260:266	arg1	hydroxyapatite					280:293	mineral ions-loaded hydroxyapatite	260:293	mineral ions-loaded hydroxyapatite (mHA)	260:299	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	7	5	theme	Chi-g-PMMA	1358:1367	arg1	scaffolds					1369:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	6	6	theme	cells	1150:1154	arg1	viability					1115:1123	viability	1115:1123	viability	1115:1123	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	6	6	theme	cells	1150:1154	arg1	attachment					1103:1112	attachment	1103:1112	attachment	1103:1112	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	6	6	theme	cells	1150:1154	arg1	proliferation					1129:1141	proliferation	1129:1141	proliferation	1129:1141	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	8	7	theme	incubation	1545:1554	arg1	h					1540:1540	25 h	1537:1540	25 h of incubation	1537:1554	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	1	8	theme	hydroxyapatite	280:293	arg1	scaffolds					189:197	composite scaffolds	179:197	composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA))	179:421	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	7	9	theme	mHA	1354:1356	arg1	scaffolds					1369:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	3	10	theme	HA	683:684	arg1	particles					686:694	the HA particles	679:694	the HA particles	679:694	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	1	11	theme	composite	179:187	arg1	scaffolds					189:197	composite scaffolds	179:197	composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA))	179:421	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	3	12	theme	good	702:705	arg1	distribution					707:718	a good distribution	700:718	a good distribution of the mHA within the Chi-g-PMMA polymer matrix	700:766	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	0	13	theme	bone	132:135	arg1	engineering					144:154	bone tissue engineering	132:154	bone tissue engineering	132:154	Fabrication of biocomposite scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering.					
30690438	1	14	theme	simulated	369:377	arg1	fluid					384:388	a simulated body fluid	367:388	a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	367:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	14	theme	simulated	369:377	arg1	SBF					391:393	SBF	391:393	SBF	391:393	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	7	15	theme	serum	1296:1300	arg1	BSA					1311:1313	BSA	1311:1313	BSA	1311:1313	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	7	15	theme	serum	1296:1300	arg1	albumin					1302:1308	bovine serum albumin	1289:1308	bovine serum albumin (BSA))	1289:1315	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	1	16	theme	fluid	384:388	arg1	Chi-g-PMMA					410:419	mHA@Chi-g-PMMA	406:419	mHA@Chi-g-PMMA	406:419	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	16	theme	fluid	384:388	arg1	solution					396:403	a simulated body fluid (SBF) solution	367:403	a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	367:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	9	17	theme	experimental	1563:1574	arg1	results					1576:1582	These experimental results	1557:1582	These experimental results	1557:1582	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	3	18	theme	mHA	727:729	arg1	incorporation					642:654	a successful incorporation	629:654	a successful incorporation of the mineral ions in the HA particles	629:694	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	3	18	theme	mHA	727:729	arg1	distribution					707:718	a good distribution	700:718	a good distribution of the mHA within the Chi-g-PMMA polymer matrix	700:766	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	6	19	theme	In	987:988	arg1	experiments					1003:1013	In vitro growth experiments	987:1013	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case	987:1085	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	6	20	attach	attachment	1103:1112	arg2	cells					1150:1154	the cells	1146:1154	the cells	1146:1154	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	6	20	attach	attachment	1103:1112	arg3	scaffolds					1163:1171	the scaffolds	1159:1171	the scaffolds	1159:1171	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	9	21	theme	@	1609:1609	arg1	scaffold					1652:1659	a scaffold	1650:1659	a scaffold for bone cells ingrowth	1650:1683	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	9	21	theme	@	1609:1609	arg1	composite					1621:1629	the mHA@Chi-g-PMMA composite	1602:1629	the mHA@Chi-g-PMMA composite	1602:1629	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	0	22	theme	chitosan-grafted-poly	85:105	arg1	inclusion					72:80	modified hydroxyapatite inclusion	48:80	modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate)	48:126	Fabrication of biocomposite scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering.					
30690438	1	23	theme	hydroxyapatite	344:357	arg1	treatment					331:339	the hydrothermal treatment	314:339	the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	314:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	0	24	theme	methyl	107:112	arg1	chitosan-grafted-poly					85:105	chitosan-grafted-poly	85:105	chitosan-grafted-poly(methyl methacrylate)	85:126	Fabrication of biocomposite scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering.					
30690438	0	24	theme	methyl	107:112	arg1	methacrylate					114:125	methyl methacrylate	107:125	methyl methacrylate	107:125	Fabrication of biocomposite scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering.					
30690438	2	25	theme	physical	465:472	arg1	properties					474:483	The physical properties	461:483	The physical properties	461:483	The physical properties, bioactivity, biological properties and their capabilities for sustained drug and protein release were studied.					
30690438	6	26	from	proliferation	1129:1141	arg1	scaffolds					1163:1171	the scaffolds	1159:1171	the scaffolds	1159:1171	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	6	27	dep	In	987:988	arg1	vitro					990:994	vitro	990:994	vitro	990:994	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	6	28	theme	osteoblast-like	1029:1043	arg1	cells					1045:1049	UMR-106 osteoblast-like cells	1021:1049	UMR-106 osteoblast-like cells	1021:1049	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	6	29	from	attachment	1103:1112	arg1	scaffolds					1163:1171	the scaffolds	1159:1171	the scaffolds	1159:1171	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	0	30	theme	biocomposite	15:26	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of biocomposite	0:26	Fabrication of biocomposite scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering.					
30690438	9	31	theme	bone	1665:1668	arg1	cells					1670:1674	bone cells	1665:1674	bone cells ingrowth	1665:1683	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	6	32	from	viability	1115:1123	arg1	scaffolds					1163:1171	the scaffolds	1159:1171	the scaffolds	1159:1171	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	3	33	theme	polymer	753:759	arg1	matrix					761:766	the Chi-g-PMMA polymer matrix	738:766	the Chi-g-PMMA polymer matrix	738:766	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	6	34	theme	growth	996:1001	arg1	experiments					1003:1013	In vitro growth experiments	987:1013	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case	987:1085	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	1	35	theme	blending	444:451	arg1	method					453:458	the blending method	440:458	the blending method	440:458	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	0	36	theme	modified	48:55	arg1	inclusion					72:80	modified hydroxyapatite inclusion	48:80	modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate)	48:126	Fabrication of biocomposite scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering.					
30690438	7	37	dep	compounds	1238:1246	arg1	drug					1260:1263	the cancer drug	1249:1263	the cancer drug	1249:1263	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	7	37	dep	compounds	1238:1246	arg1	doxorubicin					1266:1276	doxorubicin	1266:1276	doxorubicin (DOX)	1266:1282	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	8	38	theme	sustained	1473:1481	arg1	%					1507:1507	73.95%	1502:1507	73.95%	1502:1507	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	8	38	theme	sustained	1473:1481	arg1	release					1483:1489	the sustained release	1469:1489	the sustained release of DOX	1469:1496	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	8	38	theme	sustained	1473:1481	arg1	BSA					1516:1518	BSA	1516:1518	BSA	1516:1518	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	7	39	theme	compounds	1238:1246	arg1	release					1223:1229	The in vitro release	1210:1229	The in vitro release	1210:1229	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	5	40	from	formation	915:923	arg1	surface					932:938	the surface	928:938	the surface	928:938	In bioactivity studies, more apatite formation on the surface were seen after immersion in the SBF solution.					
30690438	1	41	from	treatment	331:339	arg1	Chi-g-PMMA					410:419	mHA@Chi-g-PMMA	406:419	mHA@Chi-g-PMMA	406:419	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	41	from	treatment	331:339	arg1	solution					396:403	a simulated body fluid (SBF) solution	367:403	a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	367:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	5	42	theme	SBF	973:975	arg1	solution					977:984	the SBF solution	969:984	the SBF solution	969:984	In bioactivity studies, more apatite formation on the surface were seen after immersion in the SBF solution.					
30690438	9	43	used	used	1697:1700	arg2	composite					1621:1629	the mHA@Chi-g-PMMA composite	1602:1629	the mHA@Chi-g-PMMA composite	1602:1629	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	9	43	used	used	1697:1700	arg2	scaffold					1652:1659	a scaffold	1650:1659	a scaffold for bone cells ingrowth	1650:1683	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	9	44	theme	drug	1706:1709	arg1	delivery					1711:1718	drug delivery	1706:1718	drug delivery	1706:1718	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	7	45	theme	@	1357:1357	arg1	scaffolds					1369:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	6	46	theme	mHA	1058:1060	arg1	case					1082:1085	the mHA@Chi-g-PMMA scaffold case	1054:1085	the mHA@Chi-g-PMMA scaffold case	1054:1085	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	3	47	theme	ions	671:674	arg1	incorporation					642:654	a successful incorporation	629:654	a successful incorporation of the mineral ions in the HA particles	629:694	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	3	47	theme	ions	671:674	arg1	distribution					707:718	a good distribution	700:718	a good distribution of the mHA within the Chi-g-PMMA polymer matrix	700:766	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	1	48	theme	@	409:409	arg1	Chi-g-PMMA					410:419	mHA@Chi-g-PMMA	406:419	mHA@Chi-g-PMMA	406:419	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	48	theme	@	409:409	arg1	solution					396:403	a simulated body fluid (SBF) solution	367:403	a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	367:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	49	theme	ions-loaded	268:278	arg1	mHA					296:298	mHA	296:298	mHA	296:298	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	49	theme	ions-loaded	268:278	arg1	hydroxyapatite					280:293	mineral ions-loaded hydroxyapatite	260:293	mineral ions-loaded hydroxyapatite (mHA)	260:299	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	7	50	theme	separate	1345:1352	arg1	scaffolds					1369:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	2	51	theme	sustained	548:556	arg1	drug					558:561	sustained drug	548:561	sustained drug	548:561	The physical properties, bioactivity, biological properties and their capabilities for sustained drug and protein release were studied.					
30690438	9	52	theme	bone	1731:1734	arg1	repairing					1736:1744	the bone repairing	1727:1744	the bone repairing	1727:1744	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	4	53	theme	Young	802:806	arg1	modulus					810:816	the Young's modulus	798:816	the Young's modulus	798:816	The compressive strength and the Young's modulus were 15.760 ± 0.718 and 658.452 ± 17.020 MPa, respectively.					
30690438	4	53	theme	Young	802:806	arg1	MPa					859:861	15.760 ± 0.718 and 658.452 ± 17.020 MPa	823:861	15.760 ± 0.718 and 658.452 ± 17.020 MPa	823:861	The compressive strength and the Young's modulus were 15.760 ± 0.718 and 658.452 ± 17.020 MPa, respectively.					
30690438	6	54	dep	attachment	1103:1112	arg1	the					1099:1101	the	1099:1101	the	1099:1101	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	6	55	theme	immersion	1199:1207	arg1	d					1194:1194	7 d	1192:1194	7 d of immersion	1192:1207	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	0	56	theme	tissue	137:142	arg1	engineering					144:154	bone tissue engineering	132:154	bone tissue engineering	132:154	Fabrication of biocomposite scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering.					
30690438	1	57	theme	chitosan-graft-poly	202:220	arg1	scaffolds					189:197	composite scaffolds	179:197	composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA))	179:421	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	2	58	theme	biological	499:508	arg1	properties					510:519	biological properties	499:519	biological properties	499:519	The physical properties, bioactivity, biological properties and their capabilities for sustained drug and protein release were studied.					
30690438	1	59	theme	body	379:382	arg1	fluid					384:388	a simulated body fluid	367:388	a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	367:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	59	theme	body	379:382	arg1	SBF					391:393	SBF	391:393	SBF	391:393	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	60	theme	methyl	222:227	arg1	chitosan-graft-poly					202:220	chitosan-graft-poly	202:220	chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA)	202:254	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	60	theme	methyl	222:227	arg1	methacrylate					229:240	methyl methacrylate	222:240	methyl methacrylate	222:240	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	7	61	theme	albumin	1302:1308	arg1	release					1223:1229	The in vitro release	1210:1229	The in vitro release	1210:1229	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	3	62	theme	Chi-g-PMMA	742:751	arg1	matrix					761:766	the Chi-g-PMMA polymer matrix	738:766	the Chi-g-PMMA polymer matrix	738:766	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	5	63	theme	bioactivity	881:891	arg1	studies					893:899	bioactivity studies	881:899	bioactivity studies	881:899	In bioactivity studies, more apatite formation on the surface were seen after immersion in the SBF solution.					
30690438	7	64	theme	bovine	1289:1294	arg1	BSA					1311:1313	BSA	1311:1313	BSA	1311:1313	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	7	64	theme	bovine	1289:1294	arg1	albumin					1302:1308	bovine serum albumin	1289:1308	bovine serum albumin (BSA))	1289:1315	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	9	65	used	utilized	1638:1645	arg2	composite					1621:1629	the mHA@Chi-g-PMMA composite	1602:1629	the mHA@Chi-g-PMMA composite	1602:1629	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	9	65	used	utilized	1638:1645	arg2	scaffold					1652:1659	a scaffold	1650:1659	a scaffold for bone cells ingrowth	1650:1683	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	1	66	from	solution	396:403	arg1	treatment					331:339	the hydrothermal treatment	314:339	the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	314:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	9	67	theme	mHA	1606:1608	arg1	scaffold					1652:1659	a scaffold	1650:1659	a scaffold for bone cells ingrowth	1650:1683	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	9	67	theme	mHA	1606:1608	arg1	composite					1621:1629	the mHA@Chi-g-PMMA composite	1602:1629	the mHA@Chi-g-PMMA composite	1602:1629	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	1	68	theme	present	164:170	arg1	study					172:176	the present study	160:176	the present study	160:176	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	9	69	theme	Chi-g-PMMA	1610:1619	arg1	scaffold					1652:1659	a scaffold	1650:1659	a scaffold for bone cells ingrowth	1650:1683	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	9	69	theme	Chi-g-PMMA	1610:1619	arg1	composite					1621:1629	the mHA@Chi-g-PMMA composite	1602:1629	the mHA@Chi-g-PMMA composite	1602:1629	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	6	70	theme	Chi-g-PMMA	1062:1071	arg1	case					1082:1085	the mHA@Chi-g-PMMA scaffold case	1054:1085	the mHA@Chi-g-PMMA scaffold case	1054:1085	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	7	71	dep	in	1214:1215	arg1	vitro					1217:1221	vitro	1217:1221	vitro	1217:1221	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	7	72	theme	delivery	1432:1439	arg1	vehicles					1441:1448	drug delivery vehicles	1427:1448	drug delivery vehicles	1427:1448	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	3	73	from	distribution	707:718	arg1	particles					686:694	the HA particles	679:694	the HA particles	679:694	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	5	74	theme	apatite	907:913	arg1	formation					915:923	more apatite formation	902:923	more apatite formation on the surface	902:938	In bioactivity studies, more apatite formation on the surface were seen after immersion in the SBF solution.					
30690438	7	75	theme	cancer	1253:1258	arg1	drug					1260:1263	the cancer drug	1249:1263	the cancer drug	1249:1263	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	7	75	theme	cancer	1253:1258	arg1	doxorubicin					1266:1276	doxorubicin	1266:1276	doxorubicin (DOX)	1266:1282	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	1	76	from	hydroxyapatite	344:357	arg1	Chi-g-PMMA					410:419	mHA@Chi-g-PMMA	406:419	mHA@Chi-g-PMMA	406:419	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	1	76	from	hydroxyapatite	344:357	arg1	solution					396:403	a simulated body fluid (SBF) solution	367:403	a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	367:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	7	77	theme	drug	1427:1430	arg1	vehicles					1441:1448	drug delivery vehicles	1427:1448	drug delivery vehicles	1427:1448	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	9	78	theme	cells	1670:1674	arg1	ingrowth					1676:1683	bone cells ingrowth	1665:1683	bone cells ingrowth	1665:1683	These experimental results demonstrated that the mHA@Chi-g-PMMA composite can be utilized as a scaffold for bone cells ingrowth and also be used for drug delivery during the bone repairing.					
30690438	3	79	theme	Physicochemical	597:611	arg1	analysis					613:620	Physicochemical analysis	597:620	Physicochemical analysis	597:620	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	4	80	theme	compressive	773:783	arg1	strength					785:792	The compressive strength	769:792	The compressive strength	769:792	The compressive strength and the Young's modulus were 15.760 ± 0.718 and 658.452 ± 17.020 MPa, respectively.					
30690438	4	80	theme	compressive	773:783	arg1	MPa					859:861	15.760 ± 0.718 and 658.452 ± 17.020 MPa	823:861	15.760 ± 0.718 and 658.452 ± 17.020 MPa	823:861	The compressive strength and the Young's modulus were 15.760 ± 0.718 and 658.452 ± 17.020 MPa, respectively.					
30690438	0	81	theme	hydroxyapatite	57:70	arg1	inclusion					72:80	modified hydroxyapatite inclusion	48:80	modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate)	48:126	Fabrication of biocomposite scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering.					
30690438	8	82	theme	BSA	1516:1518	arg1	%					1507:1507	73.95%	1502:1507	73.95%	1502:1507	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	8	82	theme	BSA	1516:1518	arg1	BSA					1516:1518	BSA	1516:1518	BSA	1516:1518	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	8	82	theme	BSA	1516:1518	arg1	%					1529:1529	57.27%	1524:1529	57.27%	1524:1529	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	8	82	theme	BSA	1516:1518	arg1	release					1483:1489	the sustained release	1469:1489	the sustained release of DOX	1469:1496	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	1	83	theme	hydrothermal	318:329	arg1	treatment					331:339	the hydrothermal treatment	314:339	the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)	314:420	In the present study, composite scaffolds of chitosan-graft-poly(methyl methacrylate) (Chi-g-PMMA) and mineral ions-loaded hydroxyapatite (mHA) (obtained by the hydrothermal treatment of hydroxyapatite (HA) in a simulated body fluid (SBF) solution (mHA@Chi-g-PMMA)) were prepared by the blending method.					
30690438	0	84	dep	scaffolds	28:36	arg1	made					38:41	made	38:41	scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering	28:154	Fabrication of biocomposite scaffolds made with modified hydroxyapatite inclusion of chitosan-grafted-poly(methyl methacrylate) for bone tissue engineering.					
30690438	7	85	theme	in	1214:1215	arg1	release					1223:1229	The in vitro release	1210:1229	The in vitro release	1210:1229	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	3	86	theme	successful	631:640	arg1	incorporation					642:654	a successful incorporation	629:654	a successful incorporation of the mineral ions in the HA particles	629:694	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
30690438	7	87	attach	attached	1333:1340	arg2	release					1223:1229	The in vitro release	1210:1229	The in vitro release	1210:1229	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	7	87	attach	attached	1333:1340	arg1	scaffolds					1369:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	separate mHA@Chi-g-PMMA scaffolds	1345:1377	The in vitro release of two compounds (the cancer drug, doxorubicin (DOX)) and bovine serum albumin (BSA)), which had been attached to separate mHA@Chi-g-PMMA scaffolds, were studied to determine their suitability as drug delivery vehicles.					
30690438	6	88	theme	scaffold	1073:1080	arg1	case					1082:1085	the mHA@Chi-g-PMMA scaffold case	1054:1085	the mHA@Chi-g-PMMA scaffold case	1054:1085	In vitro growth experiments using UMR-106 osteoblast-like cells on the mHA@Chi-g-PMMA scaffold case showed that the attachment, viability and proliferation of the cells on the scaffolds had improved after 7 d of immersion.					
30690438	8	89	theme	DOX	1494:1496	arg1	%					1507:1507	73.95%	1502:1507	73.95%	1502:1507	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	8	89	theme	DOX	1494:1496	arg1	release					1483:1489	the sustained release	1469:1489	the sustained release of DOX	1469:1496	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	8	89	theme	DOX	1494:1496	arg1	BSA					1516:1518	BSA	1516:1518	BSA	1516:1518	It was found that the sustained release of DOX was 73.95% and of BSA was 57.27% after 25 h of incubation.					
30690438	3	90	from	incorporation	642:654	arg1	particles					686:694	the HA particles	679:694	the HA particles	679:694	Physicochemical analysis showed a successful incorporation of the mineral ions in the HA particles and a good distribution of the mHA within the Chi-g-PMMA polymer matrix.					
31554038	11	0	theme	phenethyl	2029:2037	arg1	acetate					2039:2045	phenethyl acetate	2029:2045	phenethyl acetate	2029:2045	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	6	1	theme	gastrointestinal	1238:1253	arg1	tract					1255:1259	the simulated gastrointestinal tract	1224:1259	the simulated gastrointestinal tract	1224:1259	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	7	2	theme	10	1414:1415	arg1	%					1416:1416	%	1416:1416	%	1416:1416	Both yeasts survived at 2.5%, 5%, and 10% NaCl for 21 days and showed growth at 4 °C.					
31554038	8	3	theme	highest	1540:1546	arg1	levels					1548:1553	the highest levels	1536:1553	the highest levels of lactose consumption	1536:1576	In cheese, the single inoculum of K. lactis B10 and mixed inoculum showed the highest levels of lactose consumption.					
31554038	1	4	theme	starter	274:280	arg1	cultures					282:289	starter cultures	274:289	starter cultures of cheeses	274:300	The demand for new probiotic products has shown recent increases alongside a growing interest in studying starter cultures of cheeses.					
31554038	6	5	theme	delbrueckii	1137:1147	arg1	B14					1149:1151	T. delbrueckii B14	1134:1151	T. delbrueckii B14	1134:1151	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	0	6	dep	Kluyveromyces	12:24	arg1	lactis					26:31	Kluyveromyces lactis	12:31	Kluyveromyces lactis	12:31	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.					
31554038	9	7	theme	GC-MS	1587:1591	arg1	analysis					1593:1600	HS-SPME GC-MS analysis	1579:1600	HS-SPME GC-MS analysis of cheese samples	1579:1618	HS-SPME GC-MS analysis of cheese samples allowed the identification of 38 volatile compounds.					
31554038	0	8	theme	cheese	149:154	arg1	production					156:165	cheese production	149:165	cheese production	149:165	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.					
31554038	1	9	theme	probiotic	187:195	arg1	products					197:204	new probiotic products	183:204	new probiotic products	183:204	The demand for new probiotic products has shown recent increases alongside a growing interest in studying starter cultures of cheeses.					
31554038	3	10	theme	gastrointestinal	577:592	arg1	tracts					594:599	simulated gastrointestinal tracts	567:599	simulated gastrointestinal tracts	567:599	These two yeast strains were subjected to simulated gastrointestinal tracts and tested for self-aggregation, hydrophobicity, pathogen inhibition, antibiotic resistance, and β-galactosidase production.					
31554038	9	11	theme	samples	1612:1618	arg1	analysis					1593:1600	HS-SPME GC-MS analysis	1579:1600	HS-SPME GC-MS analysis of cheese samples	1579:1618	HS-SPME GC-MS analysis of cheese samples allowed the identification of 38 volatile compounds.					
31554038	12	12	theme	compounds	2249:2257	arg1	composition					2215:2225	the composition	2211:2225	the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum	2211:2310	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	6	13	theme	>90	1289:1291	arg1	%					1292:1292	%	1292:1292	%	1292:1292	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	1	14	theme	recent	216:221	arg1	increases					223:231	recent increases	216:231	recent increases alongside a growing interest in studying starter cultures of cheeses	216:300	The demand for new probiotic products has shown recent increases alongside a growing interest in studying starter cultures of cheeses.					
31554038	6	15	theme	>80	1183:1185	arg1	%					1186:1186	%	1186:1186	%	1186:1186	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	6	16	theme	self-aggregation	1266:1281	arg1	rates					1283:1287	self-aggregation rates	1266:1287	self-aggregation rates >90%	1266:1292	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	11	17	theme	decanoic	1865:1872	arg1	acid					1874:1877	decanoic acid	1865:1877	decanoic acid	1865:1877	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	11	18	theme	ester	1988:1992	arg1	compounds					1994:2002	the most prevalent ester compounds	1969:2002	the most prevalent ester compounds	1969:2002	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	11	18	theme	ester	1988:1992	arg1	acetate					2017:2023	isoamyl acetate	2009:2023	isoamyl acetate	2009:2023	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	11	19	theme	abundant	1889:1896	arg1	2,3-butanediol					1912:1925	2,3-butanediol	1912:1925	2,3-butanediol	1912:1925	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	11	19	theme	abundant	1889:1896	arg1	alcohols					1898:1905	the most abundant alcohols	1880:1905	the most abundant alcohols	1880:1905	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	2	20	theme	cheese	506:511	arg1	production					513:522	cheese production	506:522	cheese production	506:522	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	4	21	theme	cheese	940:945	arg1	production					947:956	cheese production	940:956	cheese production	940:956	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	12	22	theme	desirable	2230:2238	arg1	compounds					2249:2257	desirable volatile compounds	2230:2257	desirable volatile compounds	2230:2257	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	6	23	theme	lactis	1160:1165	arg1	B10					1167:1169	K. lactis B10	1157:1169	K. lactis B10	1157:1169	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	4	24	theme	yeast	730:734	arg1	strains					736:742	The yeast strains	726:742	The yeast strains	726:742	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	24	theme	yeast	730:734	arg1	cultures					927:934	single and mixed starter cultures	902:934	single and mixed starter cultures	902:934	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	6	25	theme	0.53 U/g	1352:1359	arg1	activities					1325:1334	β-galactosidase activities	1309:1334	β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively	1309:1373	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	4	26	dep	concentrations	810:823	arg1	%					842:842	10%	840:842	10%	840:842	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	26	dep	concentrations	810:823	arg1	%					829:829	2.5%	826:829	2.5%	826:829	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	26	dep	concentrations	810:823	arg1	%					833:833	5%	832:833	5%	832:833	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	6	27	theme	0.35 U/g	1339:1346	arg1	activities					1325:1334	β-galactosidase activities	1309:1334	β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively	1309:1373	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	4	28	theme	mixed	913:917	arg1	strains					736:742	The yeast strains	726:742	The yeast strains	726:742	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	28	theme	mixed	913:917	arg1	cultures					927:934	single and mixed starter cultures	902:934	single and mixed starter cultures	902:934	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	29	theme	NaCl	805:808	arg1	temperatures					859:870	multiple temperatures	850:870	multiple temperatures (4 °C and 40 °C)	850:887	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	29	theme	NaCl	805:808	arg1	concentrations					810:823	different NaCl concentrations	795:823	different NaCl concentrations (2.5%, 5%, and 10% w/v)	795:847	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	9	30	theme	volatile	1653:1660	arg1	compounds					1662:1670	38 volatile compounds	1650:1670	38 volatile compounds	1650:1670	HS-SPME GC-MS analysis of cheese samples allowed the identification of 38 volatile compounds.					
31554038	2	31	theme	Kluyveromyces	449:461	arg1	impact					407:412	impact	407:412	impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10	407:472	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	2	31	theme	Kluyveromyces	449:461	arg1	conditions					392:401	simulated gastrointestinal conditions	365:401	simulated gastrointestinal conditions	365:401	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	0	32	theme	Kluyveromyces	12:24	arg1	Survival					0:7	Survival	0:7	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.	0:166	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.					
31554038	1	33	theme	cheeses	294:300	arg1	cultures					282:289	starter cultures	274:289	starter cultures of cheeses	274:300	The demand for new probiotic products has shown recent increases alongside a growing interest in studying starter cultures of cheeses.					
31554038	2	34	theme	mixed	488:492	arg1	inocula					494:500	single and mixed inocula	477:500	single and mixed inocula for cheese production	477:522	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	0	35	theme	simulated	64:72	arg1	conditions					91:100	simulated gastrointestinal conditions	64:100	simulated gastrointestinal conditions	64:100	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.					
31554038	2	36	dep	Torulaspora	417:427	arg1	delbrueckii					429:439	Torulaspora delbrueckii B14	417:443	Torulaspora delbrueckii B14	417:443	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	2	36	dep	Torulaspora	417:427	arg1	B14					441:443	B14	441:443	Torulaspora delbrueckii B14	417:443	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	2	37	theme	Torulaspora	417:427	arg1	impact					407:412	impact	407:412	impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10	407:472	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	2	37	theme	Torulaspora	417:427	arg1	conditions					392:401	simulated gastrointestinal conditions	365:401	simulated gastrointestinal conditions	365:401	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	5	38	theme	cheese	1107:1112	arg1	process					1125:1131	the 21 days cheese maturation process	1095:1131	the 21 days cheese maturation process	1095:1131	Yeasts population levels were monitored by YPD plating and MALDI-TOF and metabolites were analyzed by HPLC and GC-MS over the course of the 21 days cheese maturation process.					
31554038	2	39	theme	single	477:482	arg1	inocula					494:500	single and mixed inocula	477:500	single and mixed inocula for cheese production	477:522	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	8	40	theme	single	1477:1482	arg1	inoculum					1484:1491	the single inoculum	1473:1491	the single inoculum of K. lactis B10 and mixed inoculum	1473:1527	In cheese, the single inoculum of K. lactis B10 and mixed inoculum showed the highest levels of lactose consumption.					
31554038	3	41	theme	antibiotic	671:680	arg1	resistance					682:691	antibiotic resistance	671:691	antibiotic resistance	671:691	These two yeast strains were subjected to simulated gastrointestinal tracts and tested for self-aggregation, hydrophobicity, pathogen inhibition, antibiotic resistance, and β-galactosidase production.					
31554038	12	42	theme	lactis	2106:2111	arg1	B10					2113:2115	K. lactis B10	2103:2115	K. lactis B10	2103:2115	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	42	theme	lactis	2106:2111	arg1	yeasts					2133:2138	interesting yeasts	2121:2138	interesting yeasts for further studies in the context of probiotics	2121:2187	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	42	theme	lactis	2106:2111	arg1	B14					2095:2097	T. delbrueckii B14	2080:2097	T. delbrueckii B14	2080:2097	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	43	from	composition	2215:2225	arg1	cheeses					2262:2268	cheeses	2262:2268	cheeses	2262:2268	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	5	44	theme	process	1125:1131	arg1	course					1085:1090	the course	1081:1090	the course of the 21 days cheese maturation process	1081:1131	Yeasts population levels were monitored by YPD plating and MALDI-TOF and metabolites were analyzed by HPLC and GC-MS over the course of the 21 days cheese maturation process.					
31554038	10	45	theme	mixed	1798:1802	arg1	inoculum					1804:1811	mixed inoculum	1798:1811	mixed inoculum	1798:1811	The highest concentrations of most of these compounds were observed after 21 days of maturation for the cheese produced with mixed inoculum.					
31554038	5	46	theme	population	966:975	arg1	levels					977:982	Yeasts population levels	959:982	Yeasts population levels	959:982	Yeasts population levels were monitored by YPD plating and MALDI-TOF and metabolites were analyzed by HPLC and GC-MS over the course of the 21 days cheese maturation process.					
31554038	8	47	theme	lactis	1499:1504	arg1	inoculum					1484:1491	the single inoculum	1473:1491	the single inoculum of K. lactis B10 and mixed inoculum	1473:1527	In cheese, the single inoculum of K. lactis B10 and mixed inoculum showed the highest levels of lactose consumption.					
31554038	3	48	theme	β-galactosidase	698:712	arg1	production					714:723	β-galactosidase production	698:723	β-galactosidase production	698:723	These two yeast strains were subjected to simulated gastrointestinal tracts and tested for self-aggregation, hydrophobicity, pathogen inhibition, antibiotic resistance, and β-galactosidase production.					
31554038	12	49	theme	delbrueckii	2083:2093	arg1	B10					2113:2115	K. lactis B10	2103:2115	K. lactis B10	2103:2115	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	49	theme	delbrueckii	2083:2093	arg1	yeasts					2133:2138	interesting yeasts	2121:2138	interesting yeasts for further studies in the context of probiotics	2121:2187	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	49	theme	delbrueckii	2083:2093	arg1	B14					2095:2097	T. delbrueckii B14	2080:2097	T. delbrueckii B14	2080:2097	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	2	50	theme	simulated	365:373	arg1	conditions					392:401	simulated gastrointestinal conditions	365:401	simulated gastrointestinal conditions	365:401	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	8	51	theme	lactose	1558:1564	arg1	consumption					1566:1576	lactose consumption	1558:1576	lactose consumption	1558:1576	In cheese, the single inoculum of K. lactis B10 and mixed inoculum showed the highest levels of lactose consumption.					
31554038	7	52	theme	%	1416:1416	arg1	NaCl					1418:1421	10% NaCl	1414:1421	10% NaCl	1414:1421	Both yeasts survived at 2.5%, 5%, and 10% NaCl for 21 days and showed growth at 4 °C.					
31554038	4	53	theme	multiple	850:857	arg1	temperatures					859:870	multiple temperatures	850:870	multiple temperatures (4 °C and 40 °C)	850:887	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	53	theme	multiple	850:857	arg1	concentrations					810:823	different NaCl concentrations	795:823	different NaCl concentrations (2.5%, 5%, and 10% w/v)	795:847	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	53	theme	multiple	850:857	arg1	40 °C					882:886	40 °C	882:886	40 °C	882:886	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	53	theme	multiple	850:857	arg1	4 °C					873:876	4 °C	873:876	4 °C	873:876	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	10	54	theme	maturation	1758:1767	arg1	21 days					1747:1753	21 days	1747:1753	21 days of maturation for the cheese produced with mixed inoculum	1747:1811	The highest concentrations of most of these compounds were observed after 21 days of maturation for the cheese produced with mixed inoculum.					
31554038	6	55	theme	simulated	1228:1236	arg1	tract					1255:1259	the simulated gastrointestinal tract	1224:1259	the simulated gastrointestinal tract	1224:1259	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	0	56	dep	Torulaspora	37:47	arg1	delbrueckii					49:59	Torulaspora delbrueckii	37:59	Torulaspora delbrueckii	37:59	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.					
31554038	1	57	theme	new	183:185	arg1	products					197:204	new probiotic products	183:204	new probiotic products	183:204	The demand for new probiotic products has shown recent increases alongside a growing interest in studying starter cultures of cheeses.					
31554038	9	58	theme	HS-SPME	1579:1585	arg1	analysis					1593:1600	HS-SPME GC-MS analysis	1579:1600	HS-SPME GC-MS analysis of cheese samples	1579:1618	HS-SPME GC-MS analysis of cheese samples allowed the identification of 38 volatile compounds.					
31554038	6	59	theme	T.	1134:1135	arg1	B14					1149:1151	T. delbrueckii B14	1134:1151	T. delbrueckii B14	1134:1151	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	12	60	from	studies	2152:2158	arg1	context					2167:2173	the context	2163:2173	the context of probiotics	2163:2187	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	10	61	theme	most	1703:1706	arg1	concentrations					1685:1698	The highest concentrations	1673:1698	The highest concentrations of most of these compounds	1673:1725	The highest concentrations of most of these compounds were observed after 21 days of maturation for the cheese produced with mixed inoculum.					
31554038	4	62	dep	%	829:829	arg1	w/v					844:846	w/v	844:846	2.5%, 5%, and 10% w/v	826:846	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	0	63	theme	mixed	130:134	arg1	inoculum					136:143	mixed inoculum	130:143	mixed inoculum	130:143	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.					
31554038	3	64	theme	simulated	567:575	arg1	tracts					594:599	simulated gastrointestinal tracts	567:599	simulated gastrointestinal tracts	567:599	These two yeast strains were subjected to simulated gastrointestinal tracts and tested for self-aggregation, hydrophobicity, pathogen inhibition, antibiotic resistance, and β-galactosidase production.					
31554038	9	65	theme	cheese	1605:1610	arg1	samples					1612:1618	cheese samples	1605:1618	cheese samples	1605:1618	HS-SPME GC-MS analysis of cheese samples allowed the identification of 38 volatile compounds.					
31554038	6	66	theme	%	1292:1292	arg1	rates					1283:1287	self-aggregation rates	1266:1287	self-aggregation rates >90%	1266:1292	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	6	67	theme	%	1186:1186	arg1	viability					1188:1196	>80% viability	1183:1196	>80% viability	1183:1196	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	11	68	theme	isoamyl	2009:2015	arg1	compounds					1994:2002	the most prevalent ester compounds	1969:2002	the most prevalent ester compounds	1969:2002	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	11	68	theme	isoamyl	2009:2015	arg1	acetate					2017:2023	isoamyl acetate	2009:2023	isoamyl acetate	2009:2023	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	10	69	theme	highest	1677:1683	arg1	concentrations					1685:1698	The highest concentrations	1673:1698	The highest concentrations of most of these compounds	1673:1725	The highest concentrations of most of these compounds were observed after 21 days of maturation for the cheese produced with mixed inoculum.					
31554038	3	70	theme	pathogen	650:657	arg1	inhibition					659:668	pathogen inhibition	650:668	pathogen inhibition	650:668	These two yeast strains were subjected to simulated gastrointestinal tracts and tested for self-aggregation, hydrophobicity, pathogen inhibition, antibiotic resistance, and β-galactosidase production.					
31554038	12	71	theme	volatile	2240:2247	arg1	compounds					2249:2257	desirable volatile compounds	2230:2257	desirable volatile compounds	2230:2257	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	72	theme	further	2144:2150	arg1	studies					2152:2158	further studies	2144:2158	further studies in the context of probiotics	2144:2187	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	0	73	theme	gastrointestinal	74:89	arg1	conditions					91:100	simulated gastrointestinal conditions	64:100	simulated gastrointestinal conditions	64:100	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.					
31554038	9	74	theme	compounds	1662:1670	arg1	identification					1632:1645	the identification	1628:1645	the identification of 38 volatile compounds	1628:1670	HS-SPME GC-MS analysis of cheese samples allowed the identification of 38 volatile compounds.					
31554038	6	75	theme	K.	1157:1158	arg1	B10					1167:1169	K. lactis B10	1157:1169	K. lactis B10	1157:1169	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	6	76	dep	showed	1176:1181	arg1	both					1171:1174	both	1171:1174	both	1171:1174	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	6	77	contain	had	1262:1264	arg2	rates					1283:1287	self-aggregation rates	1266:1287	self-aggregation rates >90%	1266:1292	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	6	77	contain	had	1262:1264	arg1	B14					1149:1151	T. delbrueckii B14	1134:1151	T. delbrueckii B14	1134:1151	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	11	78	theme	prevalent	1978:1986	arg1	compounds					1994:2002	the most prevalent ester compounds	1969:2002	the most prevalent ester compounds	1969:2002	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	11	78	theme	prevalent	1978:1986	arg1	acetate					2017:2023	isoamyl acetate	2009:2023	isoamyl acetate	2009:2023	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	5	79	theme	21 days	1099:1105	arg1	process					1125:1131	the 21 days cheese maturation process	1095:1131	the 21 days cheese maturation process	1095:1131	Yeasts population levels were monitored by YPD plating and MALDI-TOF and metabolites were analyzed by HPLC and GC-MS over the course of the 21 days cheese maturation process.					
31554038	11	80	theme	isoamyl	1948:1954	arg1	alcohol					1956:1962	isoamyl alcohol	1948:1962	isoamyl alcohol	1948:1962	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	4	81	theme	different	795:803	arg1	temperatures					859:870	multiple temperatures	850:870	multiple temperatures (4 °C and 40 °C)	850:887	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	81	theme	different	795:803	arg1	concentrations					810:823	different NaCl concentrations	795:823	different NaCl concentrations (2.5%, 5%, and 10% w/v)	795:847	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	6	82	theme	β-galactosidase	1309:1323	arg1	activities					1325:1334	β-galactosidase activities	1309:1334	β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively	1309:1373	T. delbrueckii B14 and K. lactis B10 both showed >80% viability after the passage through the simulated gastrointestinal tract, had self-aggregation rates >90%, and displayed β-galactosidase activities of 0.35 U/g and 0.53 U/g, respectively.					
31554038	2	83	dep	Kluyveromyces	449:461	arg1	lactis					463:468	Kluyveromyces lactis B10	449:472	Kluyveromyces lactis B10	449:472	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	2	83	dep	Kluyveromyces	449:461	arg1	B10					470:472	B10	470:472	Kluyveromyces lactis B10	449:472	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	4	84	theme	starter	919:925	arg1	strains					736:742	The yeast strains	726:742	The yeast strains	726:742	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	84	theme	starter	919:925	arg1	cultures					927:934	single and mixed starter cultures	902:934	single and mixed starter cultures	902:934	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	12	85	theme	probiotics	2178:2187	arg1	context					2167:2173	the context	2163:2173	the context of probiotics	2163:2187	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	86	theme	mixed	2297:2301	arg1	inoculum					2303:2310	mixed inoculum	2297:2310	mixed inoculum	2297:2310	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	11	87	theme	abundant	1823:1830	arg1	acids					1832:1836	The most abundant acids	1814:1836	The most abundant acids detected	1814:1845	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	11	87	theme	abundant	1823:1830	arg1	hexanoic					1852:1859	hexanoic	1852:1859	hexanoic	1852:1859	The most abundant acids detected were hexanoic and decanoic acid; the most abundant alcohols were 2,3-butanediol, 2-phenylethanol and isoamyl alcohol, and the most prevalent ester compounds were isoamyl acetate and phenethyl acetate.					
31554038	8	88	dep	lactis	1499:1504	arg1	B10					1506:1508	B10	1506:1508	B10	1506:1508	In cheese, the single inoculum of K. lactis B10 and mixed inoculum showed the highest levels of lactose consumption.					
31554038	8	88	dep	lactis	1499:1504	arg1	inoculum					1520:1527	mixed inoculum	1514:1527	mixed inoculum	1514:1527	In cheese, the single inoculum of K. lactis B10 and mixed inoculum showed the highest levels of lactose consumption.					
31554038	4	89	theme	single	902:907	arg1	strains					736:742	The yeast strains	726:742	The yeast strains	726:742	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	89	theme	single	902:907	arg1	cultures					927:934	single and mixed starter cultures	902:934	single and mixed starter cultures	902:934	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	2	90	theme	gastrointestinal	375:390	arg1	conditions					392:401	simulated gastrointestinal conditions	365:401	simulated gastrointestinal conditions	365:401	This study thus aims to evaluate the ability to survive under simulated gastrointestinal conditions and impact of Torulaspora delbrueckii B14 and Kluyveromyces lactis B10 as single and mixed inocula for cheese production.					
31554038	12	91	theme	interesting	2121:2131	arg1	B10					2113:2115	K. lactis B10	2103:2115	K. lactis B10	2103:2115	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	91	theme	interesting	2121:2131	arg1	yeasts					2133:2138	interesting yeasts	2121:2138	interesting yeasts for further studies in the context of probiotics	2121:2187	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	91	theme	interesting	2121:2131	arg1	B14					2095:2097	T. delbrueckii B14	2080:2097	T. delbrueckii B14	2080:2097	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	0	92	theme	Torulaspora	37:47	arg1	Survival					0:7	Survival	0:7	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.	0:166	Survival of Kluyveromyces lactis and Torulaspora delbrueckii to simulated gastrointestinal conditions and their use as single and mixed inoculum for cheese production.					
31554038	4	93	dep	temperatures	859:870	arg1	temperatures					859:870	multiple temperatures	850:870	multiple temperatures (4 °C and 40 °C)	850:887	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	93	dep	temperatures	859:870	arg1	40 °C					882:886	40 °C	882:886	40 °C	882:886	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	93	dep	temperatures	859:870	arg1	4 °C					873:876	4 °C	873:876	4 °C	873:876	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	5	94	theme	maturation	1114:1123	arg1	process					1125:1131	the 21 days cheese maturation process	1095:1131	the 21 days cheese maturation process	1095:1131	Yeasts population levels were monitored by YPD plating and MALDI-TOF and metabolites were analyzed by HPLC and GC-MS over the course of the 21 days cheese maturation process.					
31554038	5	95	theme	YPD	1002:1004	arg1	plating					1006:1012	YPD plating	1002:1012	YPD plating	1002:1012	Yeasts population levels were monitored by YPD plating and MALDI-TOF and metabolites were analyzed by HPLC and GC-MS over the course of the 21 days cheese maturation process.					
31554038	8	96	theme	K.	1496:1497	arg1	lactis					1499:1504	K. lactis B10 and mixed inoculum	1496:1527	K. lactis B10 and mixed inoculum	1496:1527	In cheese, the single inoculum of K. lactis B10 and mixed inoculum showed the highest levels of lactose consumption.					
31554038	12	97	theme	K.	2103:2104	arg1	B10					2113:2115	K. lactis B10	2103:2115	K. lactis B10	2103:2115	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	97	theme	K.	2103:2104	arg1	yeasts					2133:2138	interesting yeasts	2121:2138	interesting yeasts for further studies in the context of probiotics	2121:2187	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	97	theme	K.	2103:2104	arg1	B14					2095:2097	T. delbrueckii B14	2080:2097	T. delbrueckii B14	2080:2097	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	98	theme	T.	2080:2081	arg1	B10					2113:2115	K. lactis B10	2103:2115	K. lactis B10	2103:2115	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	98	theme	T.	2080:2081	arg1	yeasts					2133:2138	interesting yeasts	2121:2138	interesting yeasts for further studies in the context of probiotics	2121:2187	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	12	98	theme	T.	2080:2081	arg1	B14					2095:2097	T. delbrueckii B14	2080:2097	T. delbrueckii B14	2080:2097	Our results therefore show that T. delbrueckii B14 and K. lactis B10 are interesting yeasts for further studies in the context of probiotics and positively impact the composition of desirable volatile compounds in cheeses, particularly when used as mixed inoculum.					
31554038	5	99	theme	Yeasts	959:964	arg1	levels					977:982	Yeasts population levels	959:982	Yeasts population levels	959:982	Yeasts population levels were monitored by YPD plating and MALDI-TOF and metabolites were analyzed by HPLC and GC-MS over the course of the 21 days cheese maturation process.					
31554038	8	100	theme	consumption	1566:1576	arg1	levels					1548:1553	the highest levels	1536:1553	the highest levels of lactose consumption	1536:1576	In cheese, the single inoculum of K. lactis B10 and mixed inoculum showed the highest levels of lactose consumption.					
31554038	8	101	theme	mixed	1514:1518	arg1	inoculum					1520:1527	mixed inoculum	1514:1527	mixed inoculum	1514:1527	In cheese, the single inoculum of K. lactis B10 and mixed inoculum showed the highest levels of lactose consumption.					
31554038	1	102	theme	growing	245:251	arg1	interest					253:260	a growing interest	243:260	a growing interest in studying starter cultures of cheeses	243:300	The demand for new probiotic products has shown recent increases alongside a growing interest in studying starter cultures of cheeses.					
31554038	3	103	theme	yeast	535:539	arg1	strains					541:547	These two yeast strains	525:547	These two yeast strains	525:547	These two yeast strains were subjected to simulated gastrointestinal tracts and tested for self-aggregation, hydrophobicity, pathogen inhibition, antibiotic resistance, and β-galactosidase production.					
31554038	4	104	used	used	894:897	arg2	cultures					927:934	single and mixed starter cultures	902:934	single and mixed starter cultures	902:934	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31554038	4	104	used	used	894:897	arg2	strains					736:742	The yeast strains	726:742	The yeast strains	726:742	The yeast strains were also assessed for their ability to survive in different NaCl concentrations (2.5%, 5%, and 10% w/v), multiple temperatures (4 °C and 40 °C), and used as single and mixed starter cultures for cheese production.					
31525601	6	0	with	membranes	1381:1389	arg1	macrovoids					1402:1411	large macrovoids	1396:1411	large macrovoids	1396:1411	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	6	1	dep	Cells	1190:1194	arg1	adhered					1196:1202	adhered	1196:1202	adhered	1196:1202	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	3	2	theme	surface	646:652	arg1	macrovoids					654:663	(i) surface macrovoids	642:663	(i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation	642:745	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	2	theme	surface	646:652	arg1	pores					670:674	big pores	666:674	big pores	666:674	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	6	3	theme	small	1326:1330	arg1	macrovoids					1332:1341	small macrovoids	1326:1341	small macrovoids	1326:1341	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	6	4	theme	surface	1431:1437	arg1	area					1439:1442	low effective surface area	1417:1442	low effective surface area	1417:1442	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	6	5	theme	low	1417:1419	arg1	area					1439:1442	low effective surface area	1417:1442	low effective surface area	1417:1442	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	6	6	theme	100/0	1231:1235	arg1	membranes					1255:1263	PCL/CHT 100/0 and PCL/CHT 90/10 membranes	1223:1263	PCL/CHT 100/0 and PCL/CHT 90/10 membranes	1223:1263	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	3	7	dep	hMSCs	714:718	arg1	hMSCs					714:718	hMSCs invasion and proliferation	714:745	hMSCs invasion and proliferation	714:745	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	7	dep	hMSCs	714:718	arg1	proliferation					733:745	proliferation	733:745	proliferation	733:745	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	7	dep	hMSCs	714:718	arg1	invasion					720:727	invasion	720:727	invasion	720:727	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	8	8	with	hMSCs	1696:1700	arg1	size					1767:1770	surface mean macrovoid size	1744:1770	surface mean macrovoid size	1744:1770	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	8	8	with	hMSCs	1696:1700	arg1	area					1794:1797	effective surface area	1776:1797	effective surface area	1776:1797	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	4	9	theme	surface	959:965	arg1	morphology					967:976	surface morphology	959:976	surface morphology	959:976	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	7	10	theme	permissive	1515:1524	arg1	environment					1526:1536	a permissive environment	1513:1536	a permissive environment for hMSC adhesion and invasion promoting viability and metabolism, which are important for the maintenance of cell integrity	1513:1661	The scaffolds were able to create a permissive environment for hMSC adhesion and invasion promoting viability and metabolism, which are important for the maintenance of cell integrity.					
31525601	7	11	theme	cell	1648:1651	arg1	integrity					1653:1661	cell integrity	1648:1661	cell integrity	1648:1661	The scaffolds were able to create a permissive environment for hMSC adhesion and invasion promoting viability and metabolism, which are important for the maintenance of cell integrity.					
31525601	3	12	theme	CHT	581:583	arg1	membranes					592:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes	543:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology	543:639	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	12	theme	CHT	581:583	arg1	oxygen					821:826	oxygen	821:826	oxygen	821:826	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	12	theme	CHT	581:583	arg1	factors					836:842	growth factors	829:842	growth factors	829:842	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	13	theme	interconnected	753:766	arg1	network					780:786	(ii) interconnected microporous network	748:786	(ii) interconnected microporous network to transfer essential nutrients	748:818	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	2	14	theme	mass	482:485	arg1	transfer					487:494	the mass transfer	478:494	the mass transfer of nutrients	478:507	The strategy was to create three-dimensional membrane scaffolds with double porosity, able to promote the mass transfer of nutrients and to entrap cells.					
31525601	9	15	theme	diffusive	1901:1909	arg1	essential					1965:1973	essential	1965:1973	essential	1965:1973	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	9	15	theme	diffusive	1901:1909	arg1	transfer					1916:1923	an adequate diffusive mass transfer	1889:1923	an adequate diffusive mass transfer	1889:1923	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	5	16	theme	microporous	1116:1126	arg1	network					1128:1134	a microporous network	1114:1134	a microporous network	1114:1134	Membranes exhibited macrovoids connected with each other through a microporous network; macrovoids size increased by increasing the CHT wt%.					
31525601	3	17	theme	/chitosan	570:578	arg1	membranes					592:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes	543:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology	543:639	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	17	theme	/chitosan	570:578	arg1	oxygen					821:826	oxygen	821:826	oxygen	821:826	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	17	theme	/chitosan	570:578	arg1	factors					836:842	growth factors	829:842	growth factors	829:842	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	4	18	theme	mean	911:914	arg1	size					926:929	the mean macrovoid size	907:929	the mean macrovoid size	907:929	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	8	19	theme	oxygen	1720:1725	arg1	rate					1734:1737	oxygen uptake rate	1720:1737	oxygen uptake rate	1720:1737	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	8	19	theme	oxygen	1720:1725	arg1	hMSCs					1696:1700	hMSCs proliferation and oxygen uptake rate	1696:1737	hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area	1696:1797	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	1	20	theme	membrane	136:143	arg1	scaffolds					145:153	membrane scaffolds	136:153	membrane scaffolds	136:153	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	9	21	theme	nutrients	1928:1936	arg1	essential					1965:1973	essential	1965:1973	essential	1965:1973	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	9	21	theme	nutrients	1928:1936	arg1	transfer					1916:1923	an adequate diffusive mass transfer	1889:1923	an adequate diffusive mass transfer	1889:1923	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	5	22	theme	CHT	1181:1183	arg1	%					1187:1187	the CHT wt%	1177:1187	the CHT wt%	1177:1187	Membranes exhibited macrovoids connected with each other through a microporous network; macrovoids size increased by increasing the CHT wt%.					
31525601	0	23	theme	human	78:82	arg1	cells					101:105	human mesenchymal stem cells	78:105	human mesenchymal stem cells	78:105	Double porous poly (Ɛ-caprolactone)/chitosan membrane scaffolds as niches for human mesenchymal stem cells.					
31525601	1	24	theme	chemical	331:338	arg1	cues					370:373	cells all necessary chemical, mechanical and topographical cues	311:373	cells all necessary chemical, mechanical and topographical cues	311:373	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	3	25	dep	network	780:786	arg1	transfer					791:798	transfer	791:798	to transfer essential nutrients	788:818	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	26	theme	growth	829:834	arg1	membranes					592:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes	543:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology	543:639	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	26	theme	growth	829:834	arg1	factors					836:842	growth factors	829:842	growth factors	829:842	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	9	27	dep	cell	2008:2011	arg1	viability					2013:2021	viability	2013:2021	viability	2013:2021	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	9	28	theme	metabolites	1942:1952	arg1	essential					1965:1973	essential	1965:1973	essential	1965:1973	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	9	28	theme	metabolites	1942:1952	arg1	transfer					1916:1923	an adequate diffusive mass transfer	1889:1923	an adequate diffusive mass transfer	1889:1923	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	0	29	theme	stem	96:99	arg1	cells					101:105	human mesenchymal stem cells	78:105	human mesenchymal stem cells	78:105	Double porous poly (Ɛ-caprolactone)/chitosan membrane scaffolds as niches for human mesenchymal stem cells.					
31525601	1	30	theme	mechanical	341:350	arg1	cues					370:373	cells all necessary chemical, mechanical and topographical cues	311:373	cells all necessary chemical, mechanical and topographical cues	311:373	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	8	31	theme	surface	1744:1750	arg1	size					1767:1770	surface mean macrovoid size	1744:1770	surface mean macrovoid size	1744:1770	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	0	32	theme	Double	0:5	arg1	membrane					45:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane scaffolds as niches for human mesenchymal stem cells.					
31525601	1	33	theme	niches	245:250	arg1	properties					196:205	the biochemical and biophysical properties	164:205	the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches	164:250	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	8	34	theme	macrovoid	1757:1765	arg1	size					1767:1770	surface mean macrovoid size	1744:1770	surface mean macrovoid size	1744:1770	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	2	35	theme	membrane	421:428	arg1	scaffolds					430:438	three-dimensional membrane scaffolds	403:438	three-dimensional membrane scaffolds	403:438	The strategy was to create three-dimensional membrane scaffolds with double porosity, able to promote the mass transfer of nutrients and to entrap cells.					
31525601	0	36	theme	poly	14:17	arg1	membrane					45:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane scaffolds as niches for human mesenchymal stem cells.					
31525601	8	37	theme	effective	1776:1784	arg1	area					1794:1797	effective surface area	1776:1797	effective surface area	1776:1797	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	3	38	theme	microporous	768:778	arg1	network					780:786	(ii) interconnected microporous network	748:786	(ii) interconnected microporous network to transfer essential nutrients	748:818	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	6	39	theme	effective	1299:1307	arg1	area					1317:1320	a high effective surface area	1292:1320	a high effective surface area	1292:1320	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	0	40	theme	Ɛ-caprolactone	20:33	arg1	membrane					45:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane scaffolds as niches for human mesenchymal stem cells.					
31525601	6	41	dep	PCL/CHT	1349:1355	arg1	membranes					1381:1389	membranes	1381:1389	membranes with large macrovoids	1381:1411	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	4	42	theme	effective	932:940	arg1	area					950:953	effective surface area	932:953	effective surface area	932:953	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	3	43	theme	blend	586:590	arg1	membranes					592:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes	543:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology	543:639	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	43	theme	blend	586:590	arg1	oxygen					821:826	oxygen	821:826	oxygen	821:826	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	43	theme	blend	586:590	arg1	factors					836:842	growth factors	829:842	growth factors	829:842	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	0	44	theme	/chitosan	35:43	arg1	membrane					45:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane scaffolds as niches for human mesenchymal stem cells.					
31525601	4	45	theme	blend	1001:1005	arg1	composition					1007:1017	the PCL/CHT blend composition	989:1017	the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30)	989:1046	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	9	46	theme	cell	2008:2011	arg1	maintenance					1993:2003	the long-term maintenance	1979:2003	the long-term maintenance of cell viability and functions	1979:2035	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	3	47	theme	double	616:621	arg1	morphology					630:639	double porous morphology	616:639	double porous morphology	616:639	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	48	dep	membranes	592:600	arg1	network					780:786	(ii) interconnected microporous network	748:786	(ii) interconnected microporous network to transfer essential nutrients	748:818	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	48	dep	membranes	592:600	arg1	macrovoids					654:663	(i) surface macrovoids	642:663	(i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation	642:745	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	48	dep	membranes	592:600	arg1	pores					670:674	big pores	666:674	big pores	666:674	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	1	49	theme	cell	233:236	arg1	niches					245:250	human mesenchymal stem cell (hMSC) niches	210:250	human mesenchymal stem cell (hMSC) niches	210:250	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	8	50	theme	surface	1786:1792	arg1	area					1794:1797	effective surface area	1776:1797	effective surface area	1776:1797	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	1	51	theme	hMSC	239:242	arg1	niches					245:250	human mesenchymal stem cell (hMSC) niches	210:250	human mesenchymal stem cell (hMSC) niches	210:250	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	3	52	theme	big	666:668	arg1	macrovoids					654:663	(i) surface macrovoids	642:663	(i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation	642:745	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	52	theme	big	666:668	arg1	pores					670:674	big pores	666:674	big pores	666:674	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	9	53	theme	cell	1827:1830	arg1	invasion					1832:1839	the cell invasion	1823:1839	the cell invasion into the membrane	1823:1857	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	3	54	theme	poly	543:546	arg1	membranes					592:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes	543:600	poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology	543:639	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	54	theme	poly	543:546	arg1	oxygen					821:826	oxygen	821:826	oxygen	821:826	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	3	54	theme	poly	543:546	arg1	factors					836:842	growth factors	829:842	growth factors	829:842	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	6	55	theme	effective	1421:1429	arg1	area					1439:1442	low effective surface area	1417:1442	low effective surface area	1417:1442	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	7	56	theme	hMSC	1542:1545	arg1	adhesion					1547:1554	hMSC adhesion	1542:1554	hMSC adhesion	1542:1554	The scaffolds were able to create a permissive environment for hMSC adhesion and invasion promoting viability and metabolism, which are important for the maintenance of cell integrity.					
31525601	1	57	theme	biochemical	168:178	arg1	properties					196:205	the biochemical and biophysical properties	164:205	the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches	164:250	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	6	58	theme	large	1396:1400	arg1	macrovoids					1402:1411	large macrovoids	1396:1411	large macrovoids	1396:1411	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	1	59	theme	biophysical	184:194	arg1	properties					196:205	the biochemical and biophysical properties	164:205	the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches	164:250	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	5	60	theme	wt	1185:1186	arg1	%					1187:1187	the CHT wt%	1177:1187	the CHT wt%	1177:1187	Membranes exhibited macrovoids connected with each other through a microporous network; macrovoids size increased by increasing the CHT wt%.					
31525601	7	61	theme	integrity	1653:1661	arg1	maintenance					1633:1643	the maintenance	1629:1643	the maintenance of cell integrity	1629:1661	The scaffolds were able to create a permissive environment for hMSC adhesion and invasion promoting viability and metabolism, which are important for the maintenance of cell integrity.					
31525601	9	62	theme	adequate	1892:1899	arg1	essential					1965:1973	essential	1965:1973	essential	1965:1973	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	9	62	theme	adequate	1892:1899	arg1	transfer					1916:1923	an adequate diffusive mass transfer	1889:1923	an adequate diffusive mass transfer	1889:1923	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	4	63	theme	PCL/CHT	993:999	arg1	composition					1007:1017	the PCL/CHT blend composition	989:1017	the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30)	989:1046	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	9	64	theme	mass	1911:1914	arg1	essential					1965:1973	essential	1965:1973	essential	1965:1973	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	9	64	theme	mass	1911:1914	arg1	transfer					1916:1923	an adequate diffusive mass transfer	1889:1923	an adequate diffusive mass transfer	1889:1923	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	6	65	theme	membranes	1255:1263	arg1	surfaces					1211:1218	the surfaces	1207:1218	the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area	1207:1442	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	1	66	theme	cells	311:315	arg1	cues					370:373	cells all necessary chemical, mechanical and topographical cues	311:373	cells all necessary chemical, mechanical and topographical cues	311:373	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	1	67	theme	topographical	356:368	arg1	cues					370:373	cells all necessary chemical, mechanical and topographical cues	311:373	cells all necessary chemical, mechanical and topographical cues	311:373	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	0	68	theme	mesenchymal	84:94	arg1	cells					101:105	human mesenchymal stem cells	78:105	human mesenchymal stem cells	78:105	Double porous poly (Ɛ-caprolactone)/chitosan membrane scaffolds as niches for human mesenchymal stem cells.					
31525601	1	69	theme	necessary	321:329	arg1	cues					370:373	cells all necessary chemical, mechanical and topographical cues	311:373	cells all necessary chemical, mechanical and topographical cues	311:373	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	2	70	dep	able	462:465	arg1	entrap					516:521	entrap	516:521	to entrap cells	513:527	The strategy was to create three-dimensional membrane scaffolds with double porosity, able to promote the mass transfer of nutrients and to entrap cells.					
31525601	2	70	dep	able	462:465	arg1	promote					470:476	promote	470:476	to promote the mass transfer of nutrients	467:507	The strategy was to create three-dimensional membrane scaffolds with double porosity, able to promote the mass transfer of nutrients and to entrap cells.					
31525601	3	71	theme	essential	800:808	arg1	nutrients					810:818	essential nutrients	800:818	essential nutrients	800:818	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	2	72	theme	nutrients	499:507	arg1	transfer					487:494	the mass transfer	478:494	the mass transfer of nutrients	478:507	The strategy was to create three-dimensional membrane scaffolds with double porosity, able to promote the mass transfer of nutrients and to entrap cells.					
31525601	6	73	theme	90/10	1249:1253	arg1	membranes					1255:1263	PCL/CHT 100/0 and PCL/CHT 90/10 membranes	1223:1263	PCL/CHT 100/0 and PCL/CHT 90/10 membranes	1223:1263	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	8	74	theme	uptake	1727:1732	arg1	rate					1734:1737	oxygen uptake rate	1720:1737	oxygen uptake rate	1720:1737	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	8	74	theme	uptake	1727:1732	arg1	hMSCs					1696:1700	hMSCs proliferation and oxygen uptake rate	1696:1737	hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area	1696:1797	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	1	75	theme	human	210:214	arg1	niches					245:250	human mesenchymal stem cell (hMSC) niches	210:250	human mesenchymal stem cell (hMSC) niches	210:250	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	0	76	theme	porous	7:12	arg1	membrane					45:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane	0:52	Double porous poly (Ɛ-caprolactone)/chitosan membrane scaffolds as niches for human mesenchymal stem cells.					
31525601	4	77	dep	composition	1007:1017	arg1	90/10					1027:1031	90/10	1027:1031	90/10	1027:1031	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	4	77	dep	composition	1007:1017	arg1	100/0					1020:1024	100/0	1020:1024	100/0	1020:1024	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	4	77	dep	composition	1007:1017	arg1	70/30					1041:1045	70/30	1041:1045	70/30	1041:1045	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	4	77	dep	composition	1007:1017	arg1	80/20					1034:1038	80/20	1034:1038	80/20	1034:1038	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	4	78	theme	surface	942:948	arg1	area					950:953	effective surface area	932:953	effective surface area	932:953	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	8	79	theme	mean	1752:1755	arg1	size					1767:1770	surface mean macrovoid size	1744:1770	surface mean macrovoid size	1744:1770	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	2	80	theme	three-dimensional	403:419	arg1	scaffolds					430:438	three-dimensional membrane scaffolds	403:438	three-dimensional membrane scaffolds	403:438	The strategy was to create three-dimensional membrane scaffolds with double porosity, able to promote the mass transfer of nutrients and to entrap cells.					
31525601	6	81	theme	surface	1309:1315	arg1	area					1317:1320	a high effective surface area	1292:1320	a high effective surface area	1292:1320	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	4	82	theme	macrovoid	916:924	arg1	size					926:929	the mean macrovoid size	907:929	the mean macrovoid size	907:929	We varied the mean macrovoid size, effective surface area and surface morphology by varying the PCL/CHT blend composition (100/0, 90/10, 80/20, 70/30).					
31525601	9	83	theme	long-term	1983:1991	arg1	maintenance					1993:2003	the long-term maintenance	1979:2003	the long-term maintenance of cell viability and functions	1979:2035	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	1	84	theme	mesenchymal	216:226	arg1	niches					245:250	human mesenchymal stem cell (hMSC) niches	210:250	human mesenchymal stem cell (hMSC) niches	210:250	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
31525601	6	85	theme	PCL/CHT	1241:1247	arg1	membranes					1255:1263	PCL/CHT 100/0 and PCL/CHT 90/10 membranes	1223:1263	PCL/CHT 100/0 and PCL/CHT 90/10 membranes	1223:1263	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	6	86	theme	high	1294:1297	arg1	area					1317:1320	a high effective surface area	1292:1320	a high effective surface area	1292:1320	Cells adhered on the surfaces of PCL/CHT 100/0 and PCL/CHT 90/10 membranes, that are characterized by a high effective surface area and small macrovoids while PCL/CHT 80/20 and PCL/CHT 70/30 membranes with large macrovoids and low effective surface area entrapped cells inside macrovoids.					
31525601	3	87	dep	macrovoids	654:663	arg1	i					643:643	i	643:643	i	643:643	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	2	88	theme	double	445:450	arg1	porosity					452:459	double porosity	445:459	double porosity	445:459	The strategy was to create three-dimensional membrane scaffolds with double porosity, able to promote the mass transfer of nutrients and to entrap cells.					
31525601	3	89	theme	porous	623:628	arg1	morphology					630:639	double porous morphology	616:639	double porous morphology	616:639	We developed poly (Ɛ-caprolactone) (PCL)/chitosan (CHT) blend membranes consisting of double porous morphology: (i) surface macrovoids (big pores) which could be easily accessible for hMSCs invasion and proliferation; (ii) interconnected microporous network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds.					
31525601	8	90	dep	hMSCs	1696:1700	arg1	rate					1734:1737	oxygen uptake rate	1720:1737	oxygen uptake rate	1720:1737	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	8	90	dep	hMSCs	1696:1700	arg1	hMSCs					1696:1700	hMSCs proliferation and oxygen uptake rate	1696:1737	hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area	1696:1797	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	8	90	dep	hMSCs	1696:1700	arg1	proliferation					1702:1714	proliferation	1702:1714	proliferation	1702:1714	We found a relationship between hMSCs proliferation and oxygen uptake rate with surface mean macrovoid size and effective surface area.					
31525601	5	91	theme	macrovoids	1137:1146	arg1	size					1148:1151	macrovoids size	1137:1151	macrovoids size	1137:1151	Membranes exhibited macrovoids connected with each other through a microporous network; macrovoids size increased by increasing the CHT wt%.					
31525601	9	92	theme	functions	2027:2035	arg1	maintenance					1993:2003	the long-term maintenance	1979:2003	the long-term maintenance of cell viability and functions	1979:2035	The macrovoids enabled the cell invasion into the membrane and the microporosity ensured an adequate diffusive mass transfer of nutrients and metabolites, which are essential for the long-term maintenance of cell viability and functions.					
31525601	1	93	theme	stem	228:231	arg1	niches					245:250	human mesenchymal stem cell (hMSC) niches	210:250	human mesenchymal stem cell (hMSC) niches	210:250	In this paper, we developed membrane scaffolds to mimic the biochemical and biophysical properties of human mesenchymal stem cell (hMSC) niches to help direct self-renewal and proliferation providing to cells all necessary chemical, mechanical and topographical cues.					
30712935	10	0	from	supplement	1615:1624	arg1	rate					1767:1770	bite rate	1762:1770	bite rate	1762:1770	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	0	from	supplement	1615:1624	arg1	yield					1679:1683	milk yield	1674:1683	milk yield	1674:1683	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	0	from	supplement	1615:1624	arg1	lactose					1705:1711	milk lactose	1700:1711	milk lactose	1700:1711	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	0	from	supplement	1615:1624	arg1	mass					1752:1755	postgrazing herbage mass	1732:1755	postgrazing herbage mass	1732:1755	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	0	from	supplement	1615:1624	arg1	protein					1691:1697	milk protein	1686:1697	milk protein	1686:1697	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	0	from	supplement	1615:1624	arg1	number					1724:1729	lactation number	1714:1729	lactation number	1714:1729	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	0	from	supplement	1615:1624	arg1	weight					1666:1671	body weight	1661:1671	body weight	1661:1671	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	15	1	theme	models	2932:2937	arg1	development					2901:2911	the development	2897:2911	the development of HDMI estimation models	2897:2937	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	10	2	theme	barn	1655:1658	arg1	weight					1666:1671	body weight	1661:1671	body weight	1661:1671	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	15	3	theme	HDMI	2916:2919	arg1	models					2932:2937	HDMI estimation models	2916:2937	HDMI estimation models	2916:2937	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	11	4	theme	omitted	1809:1815	arg1	amounts					1817:1823	the omitted amounts	1805:1823	the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction	1805:1908	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	11	4	theme	omitted	1809:1815	arg1	intake					1833:1838	feed intake	1828:1838	feed intake in the barn and, due to the statistical procedure for model reduction	1828:1908	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	15	5	from	adaptation	2709:2718	arg1	set					2772:2774	a more comprehensive data set	2746:2774	a more comprehensive data set	2746:2774	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	11	6	theme	highest	2022:2028	arg1	accuracy					2030:2037	the highest accuracy	2018:2037	the highest accuracy retained additional variables	2018:2067	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	6	7	theme	pregrazing	994:1003	arg1	mass					1013:1016	pregrazing herbage mass	994:1016	pregrazing herbage mass	994:1016	The experiments differed in pasture size, herbage allowance, pregrazing herbage mass, supplements fed in the barn, and sward composition.					
30712935	14	8	theme	estimation	2619:2628	arg1	models					2630:2635	the HDMI estimation models	2610:2635	the HDMI estimation models	2610:2635	Under GA, the inclusion of behavioral characteristics in the HDMI estimation models did not reduce the root mean squared prediction error.					
30712935	14	9	theme	root	2656:2659	arg1	error					2685:2689	the root mean squared prediction error	2652:2689	the root mean squared prediction error	2652:2689	Under GA, the inclusion of behavioral characteristics in the HDMI estimation models did not reduce the root mean squared prediction error.					
30712935	10	10	theme	various	1502:1508	arg1	steps					1520:1524	various selection steps	1502:1524	various selection steps	1502:1524	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	13	11	theme	accurate	2385:2392	arg1	estimation					2410:2419	a sufficiently accurate individual HDMI estimation	2370:2419	a sufficiently accurate individual HDMI estimation	2370:2419	Even though behavioral characteristics alone did not allow a sufficiently accurate individual HDMI estimation, their inclusion under WSB improved estimation accuracy and represented the most valid variables for the HDMI estimation under WSB.					
30712935	1	12	theme	herbage	156:162	arg1	DMI					164:166	the individual herbage DMI	141:166	the individual herbage DMI (HDMI) of grazing dairy cows	141:195	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	1	12	theme	herbage	156:162	arg1	HDMI					169:172	HDMI	169:172	HDMI	169:172	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	11	13	theme	intake	1833:1838	arg1	amounts					1817:1823	the omitted amounts	1805:1823	the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction	1805:1908	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	11	13	theme	intake	1833:1838	arg1	intake					1833:1838	feed intake	1828:1838	feed intake in the barn and, due to the statistical procedure for model reduction	1828:1908	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	3	14	theme	model	625:629	arg1	development					631:641	model development	625:641	model development	625:641	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	6	15	theme	sward	1052:1056	arg1	composition					1058:1068	sward composition	1052:1068	sward composition	1052:1068	The experiments differed in pasture size, herbage allowance, pregrazing herbage mass, supplements fed in the barn, and sward composition.					
30712935	10	16	theme	crude	1540:1544	arg1	supplement					1615:1624	protein supplement	1607:1624	protein supplement	1607:1624	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	16	theme	crude	1540:1544	arg1	intake					1587:1592	chopped whole-plant corn silage intake	1555:1592	chopped whole-plant corn silage intake in the barn	1555:1604	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	16	theme	crude	1540:1544	arg1	variables					1420:1428	The variables	1416:1428	The variables retained for the GA model with the highest accuracy, determined through various selection steps,	1416:1525	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	16	theme	crude	1540:1544	arg1	intake					1641:1646	concentrate intake	1629:1646	concentrate intake	1629:1646	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	16	theme	crude	1540:1544	arg1	protein					1546:1552	herbage crude protein	1532:1552	herbage crude protein	1532:1552	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	12	17	theme	barn	2151:2154	arg1	time					2170:2173	the barn, total eating time	2147:2173	time	2170:2173	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	10	18	theme	milk	1686:1689	arg1	protein					1691:1697	milk protein	1686:1697	milk protein	1686:1697	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	14	19	theme	characteristics	2591:2605	arg1	inclusion					2567:2575	the inclusion	2563:2575	the inclusion of behavioral characteristics in the HDMI estimation models	2563:2635	Under GA, the inclusion of behavioral characteristics in the HDMI estimation models did not reduce the root mean squared prediction error.					
30712935	3	20	used	used	616:619	arg2	variables					560:568	all variables	556:568	all variables measured in the 4 underlying experiments	556:609	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	12	21	theme	total	2157:2161	arg1	time					2170:2173	the barn, total eating time	2147:2173	time	2170:2173	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	10	22	theme	lactation	1714:1722	arg1	number					1724:1729	lactation number	1714:1729	lactation number	1714:1729	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	0	23	theme	feed	107:110	arg1	variables					112:120	animal, behavioral, environmental, and feed variables	68:120	animal, behavioral, environmental, and feed variables	68:120	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	10	24	theme	whole-plant	1563:1573	arg1	intake					1587:1592	chopped whole-plant corn silage intake	1555:1592	chopped whole-plant corn silage intake in the barn	1555:1604	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	24	theme	whole-plant	1563:1573	arg1	protein					1546:1552	herbage crude protein	1532:1552	herbage crude protein	1532:1552	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	1	25	theme	grazing	178:184	arg1	cows					192:195	grazing dairy cows	178:195	grazing dairy cows	178:195	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	15	26	theme	data	2767:2770	arg1	set					2772:2774	a more comprehensive data set	2746:2774	a more comprehensive data set	2746:2774	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	9	27	theme	estimation	1287:1296	arg1	models					1298:1303	HDMI estimation models	1282:1303	HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB	1282:1398	Finally, HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB were developed.					
30712935	10	28	theme	herbage	1744:1750	arg1	mass					1752:1755	postgrazing herbage mass	1732:1755	postgrazing herbage mass	1732:1755	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	5	29	theme	WSB	878:880	arg1	variables					882:890	52 GA and 50 WSB variables	865:890	variables	882:890	The underlying data set was developed from 4 experiments with 52 GA and 50 WSB variables and one hundred thirty 7-d measurements.					
30712935	12	30	theme	herbage	2284:2290	arg1	concentration					2296:2308	herbage ash concentration	2284:2308	herbage ash concentration	2284:2308	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	3	31	theme	HDMI	654:657	arg1	estimation					659:668	HDMI estimation	654:668	HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB)	654:757	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	0	32	theme	cows	54:57	arg1	estimation					26:35	Herbage dry matter intake estimation	0:35	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.	0:121	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	10	33	theme	silage	1580:1585	arg1	intake					1587:1592	chopped whole-plant corn silage intake	1555:1592	chopped whole-plant corn silage intake in the barn	1555:1604	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	33	theme	silage	1580:1585	arg1	protein					1546:1552	herbage crude protein	1532:1552	herbage crude protein	1532:1552	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	13	34	theme	HDMI	2526:2529	arg1	estimation					2531:2540	the HDMI estimation	2522:2540	the HDMI estimation under WSB	2522:2550	Even though behavioral characteristics alone did not allow a sufficiently accurate individual HDMI estimation, their inclusion under WSB improved estimation accuracy and represented the most valid variables for the HDMI estimation under WSB.					
30712935	9	35	theme	minimal	1312:1318	arg1	error					1340:1344	a minimal relative prediction error	1310:1344	a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB	1310:1398	Finally, HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB were developed.					
30712935	11	36	theme	model	1894:1898	arg1	reduction					1900:1908	model reduction	1894:1908	model reduction	1894:1908	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	10	37	theme	bite	1762:1765	arg1	rate					1767:1770	bite rate	1762:1770	bite rate	1762:1770	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	14	38	from	inclusion	2567:2575	arg1	models					2630:2635	the HDMI estimation models	2610:2635	the HDMI estimation models	2610:2635	Under GA, the inclusion of behavioral characteristics in the HDMI estimation models did not reduce the root mean squared prediction error.					
30712935	9	39	theme	prediction	1329:1338	arg1	error					1340:1344	a minimal relative prediction error	1310:1344	a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB	1310:1398	Finally, HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB were developed.					
30712935	5	40	theme	7-d	915:917	arg1	measurements					919:930	one hundred thirty 7-d measurements	896:930	one hundred thirty 7-d measurements	896:930	The underlying data set was developed from 4 experiments with 52 GA and 50 WSB variables and one hundred thirty 7-d measurements.					
30712935	13	41	theme	valid	2502:2506	arg1	variables					2508:2516	the most valid variables	2493:2516	the most valid variables for the HDMI estimation under WSB	2493:2550	Even though behavioral characteristics alone did not allow a sufficiently accurate individual HDMI estimation, their inclusion under WSB improved estimation accuracy and represented the most valid variables for the HDMI estimation under WSB.					
30712935	5	42	theme	underlying	807:816	arg1	set					823:825	The underlying data set	803:825	The underlying data set	803:825	The underlying data set was developed from 4 experiments with 52 GA and 50 WSB variables and one hundred thirty 7-d measurements.					
30712935	15	43	theme	variables	2797:2805	arg1	validation					2732:2741	validation	2732:2741	validation on a more comprehensive data set	2732:2774	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	15	43	theme	variables	2797:2805	arg1	adaptation					2709:2718	adaptation	2709:2718	adaptation	2709:2718	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	15	43	theme	variables	2797:2805	arg1	inclusion					2784:2792	the inclusion	2780:2792	the inclusion of variables excluded in this study such as body condition score or gestation	2780:2870	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	11	44	theme	unconsidered	1915:1926	arg1	variables					1928:1936	the unconsidered variables	1911:1936	the unconsidered variables postgrazing herbage mass and bite rate	1911:1975	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	9	45	with	models	1298:1303	arg1	error					1340:1344	a minimal relative prediction error	1310:1344	a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB	1310:1398	Finally, HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB were developed.					
30712935	2	46	theme	influencing	333:343	arg1	variables					345:353	its multifaceted influencing variables	316:353	its multifaceted influencing variables	316:353	Estimating HDMI, with its multifaceted influencing variables, is difficult but may be attempted using animal, performance, behavior, and feed variables.					
30712935	13	47	theme	estimation	2457:2466	arg1	accuracy					2468:2475	estimation accuracy	2457:2475	estimation accuracy	2457:2475	Even though behavioral characteristics alone did not allow a sufficiently accurate individual HDMI estimation, their inclusion under WSB improved estimation accuracy and represented the most valid variables for the HDMI estimation under WSB.					
30712935	9	48	theme	%	1353:1353	arg1	error					1340:1344	a minimal relative prediction error	1310:1344	a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB	1310:1398	Finally, HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB were developed.					
30712935	7	49	theme	RumiWatch	1150:1158	arg1	system					1160:1165	the RumiWatch system	1146:1165	the RumiWatch system (Itin and Hoch GmbH, Liestal, Switzerland)	1146:1208	In all the experiments, cow behavioral characteristics were recorded using the RumiWatch system (Itin and Hoch GmbH, Liestal, Switzerland).					
30712935	0	50	theme	Herbage	0:6	arg1	estimation					26:35	Herbage dry matter intake estimation	0:35	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.	0:121	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	15	51	from	inclusion	2784:2792	arg1	set					2772:2774	a more comprehensive data set	2746:2774	a more comprehensive data set	2746:2774	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	10	52	from	intake	1587:1592	arg1	barn					1601:1604	barn	1601:1604	barn	1601:1604	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	12	53	theme	bites	2252:2256	arg1	concentration					2296:2308	herbage ash concentration	2284:2308	herbage ash concentration	2284:2308	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	12	53	theme	bites	2252:2256	arg1	number					2197:2202	number	2197:2202	number of total prehension bites	2197:2228	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	12	53	theme	bites	2252:2256	arg1	time					2170:2173	the barn, total eating time	2147:2173	time	2170:2173	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	12	53	theme	bites	2252:2256	arg1	number					2231:2236	number	2231:2236	number of prehension bites performed at pasture	2231:2277	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	10	54	theme	GA	1447:1448	arg1	model					1450:1454	the GA model	1443:1454	the GA model	1443:1454	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	0	55	theme	matter	12:17	arg1	estimation					26:35	Herbage dry matter intake estimation	0:35	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.	0:121	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	1	56	theme	herbage	242:248	arg1	use					227:229	an efficient use	214:229	an efficient use of pasture herbage as an animal feed with a range of benefits	214:291	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	10	57	theme	protein	1607:1613	arg1	supplement					1615:1624	protein supplement	1607:1624	protein supplement	1607:1624	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	57	theme	protein	1607:1613	arg1	protein					1546:1552	herbage crude protein	1532:1552	herbage crude protein	1532:1552	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	3	58	dep	explored	488:495	arg1	1					498:498	1	498:498	1	498:498	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	3	58	dep	explored	488:495	arg1	1					648:648	1	648:648	1	648:648	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	14	59	theme	squared	2666:2672	arg1	error					2685:2689	the root mean squared prediction error	2652:2689	the root mean squared prediction error	2652:2689	Under GA, the inclusion of behavioral characteristics in the HDMI estimation models did not reduce the root mean squared prediction error.					
30712935	7	60	theme	behavioral	1099:1108	arg1	characteristics					1110:1124	cow behavioral characteristics	1095:1124	cow behavioral characteristics	1095:1124	In all the experiments, cow behavioral characteristics were recorded using the RumiWatch system (Itin and Hoch GmbH, Liestal, Switzerland).					
30712935	12	61	theme	eating	2106:2111	arg1	variables					2085:2093	The additional variables	2070:2093	The additional variables	2070:2093	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	12	61	theme	eating	2106:2111	arg1	chews					2113:2117	total eating chews	2100:2117	total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration	2100:2308	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	10	62	theme	highest	1465:1471	arg1	accuracy					1473:1480	the highest accuracy	1461:1480	the highest accuracy	1461:1480	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	0	63	theme	grazing	40:46	arg1	cows					54:57	grazing dairy cows	40:57	grazing dairy cows	40:57	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	12	64	theme	bites	2224:2228	arg1	concentration					2296:2308	herbage ash concentration	2284:2308	herbage ash concentration	2284:2308	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	12	64	theme	bites	2224:2228	arg1	number					2197:2202	number	2197:2202	number of total prehension bites	2197:2228	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	12	64	theme	bites	2224:2228	arg1	time					2170:2173	the barn, total eating time	2147:2173	time	2170:2173	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	12	64	theme	bites	2224:2228	arg1	number					2231:2236	number	2231:2236	number of prehension bites performed at pasture	2231:2277	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	1	65	theme	dairy	186:190	arg1	cows					192:195	grazing dairy cows	178:195	grazing dairy cows	178:195	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	15	66	theme	body	2838:2841	arg1	score					2853:2857	body condition score	2838:2857	body condition score	2838:2857	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	12	67	theme	additional	2074:2083	arg1	variables					2085:2093	The additional variables	2070:2093	The additional variables	2070:2093	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	12	67	theme	additional	2074:2083	arg1	chews					2113:2117	total eating chews	2100:2117	total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration	2100:2308	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	14	68	theme	mean	2661:2664	arg1	error					2685:2689	the root mean squared prediction error	2652:2689	the root mean squared prediction error	2652:2689	Under GA, the inclusion of behavioral characteristics in the HDMI estimation models did not reduce the root mean squared prediction error.					
30712935	15	69	theme	estimation	2921:2930	arg1	models					2932:2937	HDMI estimation models	2916:2937	HDMI estimation models	2916:2937	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	13	70	theme	behavioral	2323:2332	arg1	characteristics					2334:2348	behavioral characteristics	2323:2348	behavioral characteristics	2323:2348	Even though behavioral characteristics alone did not allow a sufficiently accurate individual HDMI estimation, their inclusion under WSB improved estimation accuracy and represented the most valid variables for the HDMI estimation under WSB.					
30712935	2	71	theme	Estimating	294:303	arg1	HDMI					305:308	Estimating HDMI	294:308	Estimating HDMI	294:308	Estimating HDMI, with its multifaceted influencing variables, is difficult but may be attempted using animal, performance, behavior, and feed variables.					
30712935	11	72	theme	feed	1828:1831	arg1	intake					1833:1838	feed intake	1828:1838	feed intake in the barn and, due to the statistical procedure for model reduction	1828:1908	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	12	73	theme	total	2207:2211	arg1	bites					2224:2228	total prehension bites	2207:2228	total prehension bites	2207:2228	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	1	74	theme	efficient	217:225	arg1	use					227:229	an efficient use	214:229	an efficient use of pasture herbage as an animal feed with a range of benefits	214:291	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	6	75	theme	herbage	1005:1011	arg1	mass					1013:1016	pregrazing herbage mass	994:1016	pregrazing herbage mass	994:1016	The experiments differed in pasture size, herbage allowance, pregrazing herbage mass, supplements fed in the barn, and sward composition.					
30712935	11	76	theme	bite	1967:1970	arg1	rate					1972:1975	bite rate	1967:1975	bite rate	1967:1975	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	5	77	with	experiments	848:858	arg1	variables					882:890	52 GA and 50 WSB variables	865:890	variables	882:890	The underlying data set was developed from 4 experiments with 52 GA and 50 WSB variables and one hundred thirty 7-d measurements.					
30712935	5	77	with	experiments	848:858	arg1	measurements					919:930	one hundred thirty 7-d measurements	896:930	one hundred thirty 7-d measurements	896:930	The underlying data set was developed from 4 experiments with 52 GA and 50 WSB variables and one hundred thirty 7-d measurements.					
30712935	5	77	with	experiments	848:858	arg1	GA					868:869	52 GA and 50 WSB variables	865:890	GA	868:869	The underlying data set was developed from 4 experiments with 52 GA and 50 WSB variables and one hundred thirty 7-d measurements.					
30712935	10	78	theme	milk	1674:1677	arg1	yield					1679:1683	milk yield	1674:1683	milk yield	1674:1683	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	79	theme	selection	1510:1518	arg1	steps					1520:1524	various selection steps	1502:1524	various selection steps	1502:1524	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	1	80	theme	individual	145:154	arg1	DMI					164:166	the individual herbage DMI	141:166	the individual herbage DMI (HDMI) of grazing dairy cows	141:195	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	1	80	theme	individual	145:154	arg1	HDMI					169:172	HDMI	169:172	HDMI	169:172	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	10	81	from	intake	1641:1646	arg1	rate					1767:1770	bite rate	1762:1770	bite rate	1762:1770	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	81	from	intake	1641:1646	arg1	yield					1679:1683	milk yield	1674:1683	milk yield	1674:1683	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	81	from	intake	1641:1646	arg1	lactose					1705:1711	milk lactose	1700:1711	milk lactose	1700:1711	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	81	from	intake	1641:1646	arg1	mass					1752:1755	postgrazing herbage mass	1732:1755	postgrazing herbage mass	1732:1755	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	81	from	intake	1641:1646	arg1	protein					1691:1697	milk protein	1686:1697	milk protein	1686:1697	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	81	from	intake	1641:1646	arg1	number					1724:1729	lactation number	1714:1729	lactation number	1714:1729	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	81	from	intake	1641:1646	arg1	weight					1666:1671	body weight	1661:1671	body weight	1661:1671	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	11	82	with	model	2007:2011	arg1	accuracy					2030:2037	the highest accuracy	2018:2037	the highest accuracy retained additional variables	2018:2067	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	3	83	theme	underlying	588:597	arg1	experiments					599:609	the 4 underlying experiments	582:609	the 4 underlying experiments	582:609	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	14	84	theme	HDMI	2614:2617	arg1	models					2630:2635	the HDMI estimation models	2610:2635	the HDMI estimation models	2610:2635	Under GA, the inclusion of behavioral characteristics in the HDMI estimation models did not reduce the root mean squared prediction error.					
30712935	11	85	from	intake	1833:1838	arg1	barn					1847:1850	barn	1847:1850	barn	1847:1850	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	3	86	theme	approaches	472:481	arg1	types					463:467	2 types	461:467	2 types of approaches	461:481	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	13	87	theme	individual	2394:2403	arg1	estimation					2410:2419	a sufficiently accurate individual HDMI estimation	2370:2419	a sufficiently accurate individual HDMI estimation	2370:2419	Even though behavioral characteristics alone did not allow a sufficiently accurate individual HDMI estimation, their inclusion under WSB improved estimation accuracy and represented the most valid variables for the HDMI estimation under WSB.					
30712935	15	88	from	validation	2732:2741	arg1	set					2772:2774	a more comprehensive data set	2746:2774	a more comprehensive data set	2746:2774	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	6	89	theme	herbage	975:981	arg1	allowance					983:991	herbage allowance	975:991	herbage allowance	975:991	The experiments differed in pasture size, herbage allowance, pregrazing herbage mass, supplements fed in the barn, and sward composition.					
30712935	10	90	theme	herbage	1532:1538	arg1	supplement					1615:1624	protein supplement	1607:1624	protein supplement	1607:1624	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	90	theme	herbage	1532:1538	arg1	intake					1587:1592	chopped whole-plant corn silage intake	1555:1592	chopped whole-plant corn silage intake in the barn	1555:1604	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	90	theme	herbage	1532:1538	arg1	variables					1420:1428	The variables	1416:1428	The variables retained for the GA model with the highest accuracy, determined through various selection steps,	1416:1525	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	90	theme	herbage	1532:1538	arg1	intake					1641:1646	concentrate intake	1629:1646	concentrate intake	1629:1646	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	90	theme	herbage	1532:1538	arg1	protein					1546:1552	herbage crude protein	1532:1552	herbage crude protein	1532:1552	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	7	91	dep	system	1160:1165	arg1	Itin					1168:1171	Itin	1168:1171	Itin	1168:1171	In all the experiments, cow behavioral characteristics were recorded using the RumiWatch system (Itin and Hoch GmbH, Liestal, Switzerland).					
30712935	7	91	dep	system	1160:1165	arg1	GmbH					1182:1185	GmbH	1182:1185	GmbH	1182:1185	In all the experiments, cow behavioral characteristics were recorded using the RumiWatch system (Itin and Hoch GmbH, Liestal, Switzerland).					
30712935	7	91	dep	system	1160:1165	arg1	Switzerland					1197:1207	Switzerland	1197:1207	Switzerland	1197:1207	In all the experiments, cow behavioral characteristics were recorded using the RumiWatch system (Itin and Hoch GmbH, Liestal, Switzerland).					
30712935	12	92	theme	eating	2163:2168	arg1	time					2170:2173	the barn, total eating time	2147:2173	time	2170:2173	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	11	93	from	barn	1847:1850	arg1	amounts					1817:1823	the omitted amounts	1805:1823	the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction	1805:1908	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	11	93	from	barn	1847:1850	arg1	intake					1833:1838	feed intake	1828:1838	feed intake in the barn and, due to the statistical procedure for model reduction	1828:1908	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	14	94	theme	behavioral	2580:2589	arg1	characteristics					2591:2605	behavioral characteristics	2580:2605	behavioral characteristics	2580:2605	Under GA, the inclusion of behavioral characteristics in the HDMI estimation models did not reduce the root mean squared prediction error.					
30712935	10	95	theme	corn	1575:1578	arg1	intake					1587:1592	chopped whole-plant corn silage intake	1555:1592	chopped whole-plant corn silage intake in the barn	1555:1604	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	95	theme	corn	1575:1578	arg1	protein					1546:1552	herbage crude protein	1532:1552	herbage crude protein	1532:1552	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	15	96	theme	comprehensive	2753:2765	arg1	set					2772:2774	a more comprehensive data set	2746:2774	a more comprehensive data set	2746:2774	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	8	97	theme	n-alkane	1256:1263	arg1	method					1265:1270	the n-alkane method	1252:1270	the n-alkane method	1252:1270	Herbage intake was estimated by applying the n-alkane method.					
30712935	11	98	from	amounts	1817:1823	arg1	barn					1847:1850	barn	1847:1850	barn	1847:1850	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	10	99	theme	chopped	1555:1561	arg1	intake					1587:1592	chopped whole-plant corn silage intake	1555:1592	chopped whole-plant corn silage intake in the barn	1555:1604	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	99	theme	chopped	1555:1561	arg1	protein					1546:1552	herbage crude protein	1532:1552	herbage crude protein	1532:1552	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	9	100	theme	HDMI	1282:1285	arg1	models					1298:1303	HDMI estimation models	1282:1303	HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB	1282:1398	Finally, HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB were developed.					
30712935	10	101	theme	milk	1700:1703	arg1	lactose					1705:1711	milk lactose	1700:1711	milk lactose	1700:1711	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	3	102	theme	supplements	725:735	arg1	supplements					725:735	supplements	725:735	supplements fed in the barn (WSB)	725:757	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	3	102	theme	supplements	725:735	arg1	amount					715:720	the amount	711:720	the amount of supplements fed in the barn (WSB)	711:757	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	12	103	theme	ash	2292:2294	arg1	concentration					2296:2308	herbage ash concentration	2284:2308	herbage ash concentration	2284:2308	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	10	104	theme	postgrazing	1732:1742	arg1	mass					1752:1755	postgrazing herbage mass	1732:1755	postgrazing herbage mass	1732:1755	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	3	105	theme	HDMI	504:507	arg1	estimation					509:518	HDMI estimation	504:518	HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development	504:641	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	11	106	theme	herbage	1950:1956	arg1	mass					1958:1961	herbage mass	1950:1961	herbage mass	1950:1961	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	0	107	theme	animal	68:73	arg1	variables					112:120	animal, behavioral, environmental, and feed variables	68:120	animal, behavioral, environmental, and feed variables	68:120	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	9	108	theme	relative	1320:1327	arg1	error					1340:1344	a minimal relative prediction error	1310:1344	a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB	1310:1398	Finally, HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB were developed.					
30712935	0	109	theme	behavioral	76:85	arg1	variables					112:120	animal, behavioral, environmental, and feed variables	68:120	animal, behavioral, environmental, and feed variables	68:120	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	1	110	theme	benefits	284:291	arg1	range					275:279	a range	273:279	a range of benefits	273:291	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	4	111	theme	models	782:787	arg1	accuracy					764:771	The accuracy	760:771	The accuracy of these models	760:787	The accuracy of these models was assessed.					
30712935	11	112	theme	additional	2048:2057	arg1	variables					2059:2067	additional variables	2048:2067	additional variables	2048:2067	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	0	113	theme	environmental	88:100	arg1	variables					112:120	animal, behavioral, environmental, and feed variables	68:120	animal, behavioral, environmental, and feed variables	68:120	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	5	114	theme	data	818:821	arg1	set					823:825	The underlying data set	803:825	The underlying data set	803:825	The underlying data set was developed from 4 experiments with 52 GA and 50 WSB variables and one hundred thirty 7-d measurements.					
30712935	2	115	theme	feed	431:434	arg1	variables					436:444	feed variables	431:444	feed variables	431:444	Estimating HDMI, with its multifaceted influencing variables, is difficult but may be attempted using animal, performance, behavior, and feed variables.					
30712935	12	116	theme	prehension	2241:2250	arg1	bites					2252:2256	prehension bites	2241:2256	prehension bites performed at pasture	2241:2277	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	2	117	theme	multifaceted	320:331	arg1	variables					345:353	its multifaceted influencing variables	316:353	its multifaceted influencing variables	316:353	Estimating HDMI, with its multifaceted influencing variables, is difficult but may be attempted using animal, performance, behavior, and feed variables.					
30712935	0	118	theme	dry	8:10	arg1	estimation					26:35	Herbage dry matter intake estimation	0:35	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.	0:121	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	15	119	theme	condition	2843:2851	arg1	score					2853:2857	body condition score	2838:2857	body condition score	2838:2857	Finally, further adaptation, as well as validation on a more comprehensive data set and the inclusion of variables excluded in this study such as body condition score or gestation, should be considered in the development of HDMI estimation models.					
30712935	0	120	theme	intake	19:24	arg1	estimation					26:35	Herbage dry matter intake estimation	0:35	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.	0:121	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	1	121	theme	pasture	234:240	arg1	herbage					242:248	pasture herbage	234:248	pasture herbage	234:248	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	14	122	theme	prediction	2674:2683	arg1	error					2685:2689	the root mean squared prediction error	2652:2689	the root mean squared prediction error	2652:2689	Under GA, the inclusion of behavioral characteristics in the HDMI estimation models did not reduce the root mean squared prediction error.					
30712935	7	123	theme	cow	1095:1097	arg1	characteristics					1110:1124	cow behavioral characteristics	1095:1124	cow behavioral characteristics	1095:1124	In all the experiments, cow behavioral characteristics were recorded using the RumiWatch system (Itin and Hoch GmbH, Liestal, Switzerland).					
30712935	3	124	theme	global	528:533	arg1	GA					545:546	GA	545:546	GA	545:546	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	3	124	theme	global	528:533	arg1	approach					535:542	a global approach	526:542	a global approach (GA)	526:547	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	0	125	theme	dairy	48:52	arg1	cows					54:57	grazing dairy cows	40:57	grazing dairy cows	40:57	Herbage dry matter intake estimation of grazing dairy cows based on animal, behavioral, environmental, and feed variables.					
30712935	9	126	theme	%	1380:1380	arg1	error					1340:1344	a minimal relative prediction error	1310:1344	a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB	1310:1398	Finally, HDMI estimation models with a minimal relative prediction error of 11.1% for use under GA and 13.2% for use under WSB were developed.					
30712935	6	127	theme	pasture	961:967	arg1	size					969:972	pasture size	961:972	pasture size	961:972	The experiments differed in pasture size, herbage allowance, pregrazing herbage mass, supplements fed in the barn, and sward composition.					
30712935	11	128	theme	statistical	1868:1878	arg1	procedure					1880:1888	the statistical procedure	1864:1888	the statistical procedure for model reduction	1864:1908	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	3	129	from	estimation	659:668	arg1	approach					676:683	an approach	673:683	an approach	673:683	In our study, 2 types of approaches were explored: 1 for HDMI estimation under a global approach (GA), where all variables measured in the 4 underlying experiments were used for model development, and 1 for HDMI estimation in an approach without information about the amount of supplements fed in the barn (WSB).					
30712935	12	130	theme	prehension	2213:2222	arg1	bites					2224:2228	total prehension bites	2207:2228	total prehension bites	2207:2228	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	10	131	theme	concentrate	1629:1639	arg1	intake					1641:1646	concentrate intake	1629:1646	concentrate intake	1629:1646	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	10	131	theme	concentrate	1629:1639	arg1	protein					1546:1552	herbage crude protein	1532:1552	herbage crude protein	1532:1552	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	8	132	theme	Herbage	1211:1217	arg1	intake					1219:1224	Herbage intake	1211:1224	Herbage intake	1211:1224	Herbage intake was estimated by applying the n-alkane method.					
30712935	1	133	theme	cows	192:195	arg1	DMI					164:166	the individual herbage DMI	141:166	the individual herbage DMI (HDMI) of grazing dairy cows	141:195	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	1	133	theme	cows	192:195	arg1	HDMI					169:172	HDMI	169:172	HDMI	169:172	Information about the individual herbage DMI (HDMI) of grazing dairy cows is important for an efficient use of pasture herbage as an animal feed with a range of benefits.					
30712935	2	134	with	HDMI	305:308	arg1	variables					345:353	its multifaceted influencing variables	316:353	its multifaceted influencing variables	316:353	Estimating HDMI, with its multifaceted influencing variables, is difficult but may be attempted using animal, performance, behavior, and feed variables.					
30712935	11	135	theme	WSB	2003:2005	arg1	model					2007:2011	the WSB model	1999:2011	the WSB model with the highest accuracy retained additional variables	1999:2067	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	11	135	theme	WSB	2003:2005	arg1	pasture					1990:1996	pasture	1990:1996	pasture	1990:1996	Instead of the omitted amounts of feed intake in the barn and, due to the statistical procedure for model reduction, the unconsidered variables postgrazing herbage mass and bite rate performed at pasture, the WSB model with the highest accuracy retained additional variables.					
30712935	12	136	theme	total	2100:2104	arg1	variables					2085:2093	The additional variables	2070:2093	The additional variables	2070:2093	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	12	136	theme	total	2100:2104	arg1	chews					2113:2117	total eating chews	2100:2117	total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration	2100:2308	The additional variables were total eating chews performed at pasture and in the barn, total eating time performed at pasture, number of total prehension bites, number of prehension bites performed at pasture, and herbage ash concentration.					
30712935	10	137	theme	body	1661:1664	arg1	weight					1666:1671	body weight	1661:1671	body weight	1661:1671	The variables retained for the GA model with the highest accuracy, determined through various selection steps, were herbage crude protein, chopped whole-plant corn silage intake in the barn, protein supplement or concentrate intake in the barn, body weight, milk yield, milk protein, milk lactose, lactation number, postgrazing herbage mass, and bite rate performed at pasture.					
30712935	13	138	theme	HDMI	2405:2408	arg1	estimation					2410:2419	a sufficiently accurate individual HDMI estimation	2370:2419	a sufficiently accurate individual HDMI estimation	2370:2419	Even though behavioral characteristics alone did not allow a sufficiently accurate individual HDMI estimation, their inclusion under WSB improved estimation accuracy and represented the most valid variables for the HDMI estimation under WSB.					
30583005	9	0	theme	Candida	1170:1176	arg1	albicans					1138:1145	Candida albicans	1130:1145	Candida albicans	1130:1145	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	9	0	theme	Candida	1170:1176	arg1	krusei					1178:1183	Candida krusei	1170:1183	Candida krusei	1170:1183	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	5	1	with	similarities	650:661	arg1	proteins					682:689	Viridiplantae proteins	668:689	Viridiplantae proteins	668:689	No sequence similarities with Viridiplantae proteins were observed.					
30583005	7	2	theme	PeRoL	880:884	arg1	Trehalose					825:833	Trehalose	825:833	Trehalose	825:833	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	7	2	theme	PeRoL	880:884	arg1	inhibitor					867:875	an endogenous inhibitor	853:875	an endogenous inhibitor of PeRoL present in the roots	853:905	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	0	3	theme	antifungal	82:91	arg1	activities					93:102	antibacterial and antifungal activities	64:102	activities	93:102	Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities.					
30583005	11	4	contain	contain	1438:1444	arg2	lectin					1499:1504	a trehalose-binding, thermostable, and antimicrobial lectin	1446:1504	a trehalose-binding, thermostable, and antimicrobial lectin	1446:1504	In conclusion, the roots of P. elatior contain a trehalose-binding, thermostable, and antimicrobial lectin.					
30583005	11	4	contain	contain	1438:1444	arg1	conclusion					1402:1411	conclusion	1402:1411	conclusion	1402:1411	In conclusion, the roots of P. elatior contain a trehalose-binding, thermostable, and antimicrobial lectin.					
30583005	11	4	contain	contain	1438:1444	arg1	roots					1418:1422	the roots	1414:1422	the roots of P. elatior	1414:1436	In conclusion, the roots of P. elatior contain a trehalose-binding, thermostable, and antimicrobial lectin.					
30583005	11	4	contain	contain	1438:1444	arg2	roots					1418:1422	the roots	1414:1422	the roots of P. elatior	1414:1436	In conclusion, the roots of P. elatior contain a trehalose-binding, thermostable, and antimicrobial lectin.					
30583005	8	5	theme	inhibitory	1034:1043	arg1	concentration					1045:1057	minimal inhibitory concentration	1026:1057	minimal inhibitory concentration of 8.1	1026:1064	Bacteriostatic activity was detected against Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus (minimal inhibitory concentration of 8.1, 32.5 and 4.06 μg/mL, respectively).					
30583005	11	6	theme	trehalose-binding	1448:1464	arg1	lectin					1499:1504	a trehalose-binding, thermostable, and antimicrobial lectin	1446:1504	a trehalose-binding, thermostable, and antimicrobial lectin	1446:1504	In conclusion, the roots of P. elatior contain a trehalose-binding, thermostable, and antimicrobial lectin.					
30583005	1	7	theme	several	186:192	arg1	action					240:245	antimicrobial action	226:245	antimicrobial action	226:245	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	1	7	theme	several	186:192	arg1	functions					205:213	several biological functions	186:213	several biological functions	186:213	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	7	8	theme	endogenous	856:865	arg1	Trehalose					825:833	Trehalose	825:833	Trehalose	825:833	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	7	8	theme	endogenous	856:865	arg1	inhibitor					867:875	an endogenous inhibitor	853:875	an endogenous inhibitor of PeRoL present in the roots	853:905	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	4	9	theme	molecular	535:543	arg1	mass					545:548	native molecular mass	528:548	native molecular mass of 33 kDa, pI 3.8	528:566	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	5	10	theme	Viridiplantae	668:680	arg1	proteins					682:689	Viridiplantae proteins	668:689	Viridiplantae proteins	668:689	No sequence similarities with Viridiplantae proteins were observed.					
30583005	7	11	from	roots	901:905	arg1	present					886:892	present	886:892	present	886:892	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	10	12	theme	blood	1330:1334	arg1	PBMCs					1355:1359	PBMCs	1355:1359	PBMCs	1355:1359	The lectin (100 μg/mL) was not cytotoxic to human peripheral blood mononuclear cells (PBMCs) and did not show hemolytic activity.					
30583005	10	12	theme	blood	1330:1334	arg1	cells					1348:1352	human peripheral blood mononuclear cells	1313:1352	human peripheral blood mononuclear cells (PBMCs)	1313:1360	The lectin (100 μg/mL) was not cytotoxic to human peripheral blood mononuclear cells (PBMCs) and did not show hemolytic activity.					
30583005	7	13	attach	present	886:892	arg2	Trehalose					825:833	Trehalose	825:833	Trehalose	825:833	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	7	13	attach	present	886:892	arg2	inhibitor					867:875	an endogenous inhibitor	853:875	an endogenous inhibitor of PeRoL present in the roots	853:905	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	7	13	attach	present	886:892	arg1	roots					901:905	the roots	897:905	the roots	897:905	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	8	14	dep	concentration	1045:1057	arg1	32.5					1067:1070	32.5	1067:1070	32.5	1067:1070	Bacteriostatic activity was detected against Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus (minimal inhibitory concentration of 8.1, 32.5 and 4.06 μg/mL, respectively).					
30583005	8	14	dep	concentration	1045:1057	arg1	4.06 μg/mL					1076:1085	4.06 μg/mL	1076:1085	4.06 μg/mL	1076:1085	Bacteriostatic activity was detected against Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus (minimal inhibitory concentration of 8.1, 32.5 and 4.06 μg/mL, respectively).					
30583005	1	15	contain	have	181:184	arg1	Lectins					105:111	Lectins	105:111	Lectins	105:111	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	1	15	contain	have	181:184	arg1	proteins					138:145	carbohydrate-binding proteins	117:145	carbohydrate-binding proteins broadly distributed in plants	117:175	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	1	15	contain	have	181:184	arg2	action					240:245	antimicrobial action	226:245	antimicrobial action	226:245	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	1	15	contain	have	181:184	arg2	functions					205:213	several biological functions	186:213	several biological functions	186:213	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	9	16	theme	Candida	1190:1196	arg1	albicans					1138:1145	Candida albicans	1130:1145	Candida albicans	1130:1145	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	9	16	theme	Candida	1190:1196	arg1	tropicalis					1198:1207	Candida tropicalis	1190:1207	Candida tropicalis	1190:1207	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	2	17	theme	biotechnological	317:332	arg1	potential					334:342	biotechnological potential	317:342	biotechnological potential	317:342	Portulaca elatior is a Caatinga plant whose chemical composition and biotechnological potential have not been extensively studied.					
30583005	1	18	theme	biological	194:203	arg1	action					240:245	antimicrobial action	226:245	antimicrobial action	226:245	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	1	18	theme	biological	194:203	arg1	functions					205:213	several biological functions	186:213	several biological functions	186:213	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	0	19	theme	Portulaca	0:8	arg1	root					18:21	Portulaca elatior root	0:21	Portulaca elatior root	0:21	Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities.					
30583005	6	20	theme	hemagglutinating	716:731	arg1	HA					743:744	HA	743:744	HA	743:744	The PeRoL hemagglutinating activity (HA) was not affected by heating and was detected in a pH ranging from 4.0 to 8.0.					
30583005	6	20	theme	hemagglutinating	716:731	arg1	activity					733:740	The PeRoL hemagglutinating activity	706:740	The PeRoL hemagglutinating activity (HA)	706:745	The PeRoL hemagglutinating activity (HA) was not affected by heating and was detected in a pH ranging from 4.0 to 8.0.					
30583005	0	21	theme	elatior	10:16	arg1	root					18:21	Portulaca elatior root	0:21	Portulaca elatior root	0:21	Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities.					
30583005	6	22	located	detected	783:790	arg2	HA					743:744	HA	743:744	HA	743:744	The PeRoL hemagglutinating activity (HA) was not affected by heating and was detected in a pH ranging from 4.0 to 8.0.					
30583005	6	22	located	detected	783:790	arg2	activity					733:740	The PeRoL hemagglutinating activity	706:740	The PeRoL hemagglutinating activity (HA)	706:745	The PeRoL hemagglutinating activity (HA) was not affected by heating and was detected in a pH ranging from 4.0 to 8.0.					
30583005	6	22	located	detected	783:790	arg1	pH					797:798	a pH	795:798	a pH ranging from 4.0 to 8.0	795:822	The PeRoL hemagglutinating activity (HA) was not affected by heating and was detected in a pH ranging from 4.0 to 8.0.					
30583005	0	23	contain	contains	23:30	arg1	root					18:21	Portulaca elatior root	0:21	Portulaca elatior root	0:21	Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities.					
30583005	0	23	contain	contains	23:30	arg2	lectin					52:57	a trehalose-binding lectin	32:57	a trehalose-binding lectin with antibacterial and antifungal activities	32:102	Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities.					
30583005	7	24	from	present	886:892	arg1	roots					901:905	the roots	897:905	the roots	897:905	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	6	25	theme	PeRoL	710:714	arg1	HA					743:744	HA	743:744	HA	743:744	The PeRoL hemagglutinating activity (HA) was not affected by heating and was detected in a pH ranging from 4.0 to 8.0.					
30583005	6	25	theme	PeRoL	710:714	arg1	activity					733:740	The PeRoL hemagglutinating activity	706:740	The PeRoL hemagglutinating activity (HA)	706:745	The PeRoL hemagglutinating activity (HA) was not affected by heating and was detected in a pH ranging from 4.0 to 8.0.					
30583005	3	26	theme	elatior	423:429	arg1	extract					436:442	P. elatior root extract	420:442	P. elatior root extract	420:442	In this work, a lectin was isolated from P. elatior root extract and evaluated for antimicrobial activity.					
30583005	6	27	dep	8.0	820:822	arg1	to					817:818	to	817:818	to	817:818	The PeRoL hemagglutinating activity (HA) was not affected by heating and was detected in a pH ranging from 4.0 to 8.0.					
30583005	5	28	theme	sequence	641:648	arg1	similarities					650:661	No sequence similarities	638:661	No sequence similarities with Viridiplantae proteins	638:689	No sequence similarities with Viridiplantae proteins were observed.					
30583005	8	29	theme	minimal	1026:1032	arg1	concentration					1045:1057	minimal inhibitory concentration	1026:1057	minimal inhibitory concentration of 8.1	1026:1064	Bacteriostatic activity was detected against Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus (minimal inhibitory concentration of 8.1, 32.5 and 4.06 μg/mL, respectively).					
30583005	11	30	theme	elatior	1430:1436	arg1	roots					1418:1422	the roots	1414:1422	the roots of P. elatior	1414:1436	In conclusion, the roots of P. elatior contain a trehalose-binding, thermostable, and antimicrobial lectin.					
30583005	3	31	theme	antimicrobial	462:474	arg1	activity					476:483	antimicrobial activity	462:483	antimicrobial activity	462:483	In this work, a lectin was isolated from P. elatior root extract and evaluated for antimicrobial activity.					
30583005	10	32	theme	mononuclear	1336:1346	arg1	PBMCs					1355:1359	PBMCs	1355:1359	PBMCs	1355:1359	The lectin (100 μg/mL) was not cytotoxic to human peripheral blood mononuclear cells (PBMCs) and did not show hemolytic activity.					
30583005	10	32	theme	mononuclear	1336:1346	arg1	cells					1348:1352	human peripheral blood mononuclear cells	1313:1352	human peripheral blood mononuclear cells (PBMCs)	1313:1360	The lectin (100 μg/mL) was not cytotoxic to human peripheral blood mononuclear cells (PBMCs) and did not show hemolytic activity.					
30583005	1	33	theme	antimicrobial	226:238	arg1	action					240:245	antimicrobial action	226:245	antimicrobial action	226:245	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	10	34	theme	peripheral	1319:1328	arg1	PBMCs					1355:1359	PBMCs	1355:1359	PBMCs	1355:1359	The lectin (100 μg/mL) was not cytotoxic to human peripheral blood mononuclear cells (PBMCs) and did not show hemolytic activity.					
30583005	10	34	theme	peripheral	1319:1328	arg1	cells					1348:1352	human peripheral blood mononuclear cells	1313:1352	human peripheral blood mononuclear cells (PBMCs)	1313:1360	The lectin (100 μg/mL) was not cytotoxic to human peripheral blood mononuclear cells (PBMCs) and did not show hemolytic activity.					
30583005	4	35	theme	P.	490:491	arg1	PeRoL					514:518	PeRoL	514:518	PeRoL	514:518	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	4	35	theme	P.	490:491	arg1	lectin					506:511	The P. elatior root lectin	486:511	The P. elatior root lectin (PeRoL)	486:519	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	1	36	theme	carbohydrate-binding	117:136	arg1	Lectins					105:111	Lectins	105:111	Lectins	105:111	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	1	36	theme	carbohydrate-binding	117:136	arg1	proteins					138:145	carbohydrate-binding proteins	117:145	carbohydrate-binding proteins broadly distributed in plants	117:175	Lectins are carbohydrate-binding proteins broadly distributed in plants and have several biological functions, including antimicrobial action.					
30583005	0	37	theme	trehalose-binding	34:50	arg1	lectin					52:57	a trehalose-binding lectin	32:57	a trehalose-binding lectin with antibacterial and antifungal activities	32:102	Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities.					
30583005	9	38	theme	minimal	1223:1229	arg1	concentration					1242:1254	a minimal fungicidal concentration	1221:1254	a minimal fungicidal concentration of 16 μg/mL	1221:1266	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	2	39	theme	Caatinga	271:278	arg1	elatior					258:264	Portulaca elatior	248:264	Portulaca elatior	248:264	Portulaca elatior is a Caatinga plant whose chemical composition and biotechnological potential have not been extensively studied.					
30583005	2	39	theme	Caatinga	271:278	arg1	plant					280:284	a Caatinga plant	269:284	a Caatinga plant whose chemical composition and biotechnological potential have not been extensively studied	269:376	Portulaca elatior is a Caatinga plant whose chemical composition and biotechnological potential have not been extensively studied.					
30583005	9	40	dep	parapsilosis	1156:1167	arg1	cells					1209:1213	cells	1209:1213	cells	1209:1213	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	11	41	theme	P.	1427:1428	arg1	elatior					1430:1436	P. elatior	1427:1436	P. elatior	1427:1436	In conclusion, the roots of P. elatior contain a trehalose-binding, thermostable, and antimicrobial lectin.					
30583005	4	42	theme	33 kDa	553:558	arg1	mass					545:548	native molecular mass	528:548	native molecular mass of 33 kDa, pI 3.8	528:566	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	9	43	theme	fungicidal	1231:1240	arg1	concentration					1242:1254	a minimal fungicidal concentration	1221:1254	a minimal fungicidal concentration of 16 μg/mL	1221:1266	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	4	44	theme	root	501:504	arg1	PeRoL					514:518	PeRoL	514:518	PeRoL	514:518	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	4	44	theme	root	501:504	arg1	lectin					506:511	The P. elatior root lectin	486:511	The P. elatior root lectin (PeRoL)	486:519	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	8	45	theme	Bacteriostatic	908:921	arg1	activity					923:930	Bacteriostatic activity	908:930	Bacteriostatic activity	908:930	Bacteriostatic activity was detected against Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus (minimal inhibitory concentration of 8.1, 32.5 and 4.06 μg/mL, respectively).					
30583005	3	46	theme	root	431:434	arg1	extract					436:442	P. elatior root extract	420:442	P. elatior root extract	420:442	In this work, a lectin was isolated from P. elatior root extract and evaluated for antimicrobial activity.					
30583005	4	47	theme	elatior	493:499	arg1	PeRoL					514:518	PeRoL	514:518	PeRoL	514:518	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	4	47	theme	elatior	493:499	arg1	lectin					506:511	The P. elatior root lectin	486:511	The P. elatior root lectin (PeRoL)	486:519	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	9	48	theme	Candida	1130:1136	arg1	albicans					1138:1145	Candida albicans	1130:1145	Candida albicans	1130:1145	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	9	48	theme	Candida	1130:1136	arg1	parapsilosis					1156:1167	Candida parapsilosis	1148:1167	Candida parapsilosis	1148:1167	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	9	48	theme	Candida	1130:1136	arg1	krusei					1178:1183	Candida krusei	1170:1183	Candida krusei	1170:1183	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	9	48	theme	Candida	1130:1136	arg1	tropicalis					1198:1207	Candida tropicalis	1190:1207	Candida tropicalis	1190:1207	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	2	49	theme	Portulaca	248:256	arg1	elatior					258:264	Portulaca elatior	248:264	Portulaca elatior	248:264	Portulaca elatior is a Caatinga plant whose chemical composition and biotechnological potential have not been extensively studied.					
30583005	2	49	theme	Portulaca	248:256	arg1	plant					280:284	a Caatinga plant	269:284	a Caatinga plant whose chemical composition and biotechnological potential have not been extensively studied	269:376	Portulaca elatior is a Caatinga plant whose chemical composition and biotechnological potential have not been extensively studied.					
30583005	9	50	theme	albicans	1138:1145	arg1	death					1121:1125	the death	1117:1125	the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells	1117:1213	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	8	51	theme	8.1	1062:1064	arg1	concentration					1045:1057	minimal inhibitory concentration	1026:1057	minimal inhibitory concentration of 8.1	1026:1064	Bacteriostatic activity was detected against Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus (minimal inhibitory concentration of 8.1, 32.5 and 4.06 μg/mL, respectively).					
30583005	10	52	theme	hemolytic	1379:1387	arg1	activity					1389:1396	hemolytic activity	1379:1396	hemolytic activity	1379:1396	The lectin (100 μg/mL) was not cytotoxic to human peripheral blood mononuclear cells (PBMCs) and did not show hemolytic activity.					
30583005	9	53	theme	16 μg/mL	1259:1266	arg1	concentration					1242:1254	a minimal fungicidal concentration	1221:1254	a minimal fungicidal concentration of 16 μg/mL	1221:1266	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	10	54	theme	human	1313:1317	arg1	PBMCs					1355:1359	PBMCs	1355:1359	PBMCs	1355:1359	The lectin (100 μg/mL) was not cytotoxic to human peripheral blood mononuclear cells (PBMCs) and did not show hemolytic activity.					
30583005	10	54	theme	human	1313:1317	arg1	cells					1348:1352	human peripheral blood mononuclear cells	1313:1352	human peripheral blood mononuclear cells (PBMCs)	1313:1360	The lectin (100 μg/mL) was not cytotoxic to human peripheral blood mononuclear cells (PBMCs) and did not show hemolytic activity.					
30583005	11	55	theme	thermostable	1467:1478	arg1	lectin					1499:1504	a trehalose-binding, thermostable, and antimicrobial lectin	1446:1504	a trehalose-binding, thermostable, and antimicrobial lectin	1446:1504	In conclusion, the roots of P. elatior contain a trehalose-binding, thermostable, and antimicrobial lectin.					
30583005	2	56	theme	chemical	292:299	arg1	composition					301:311	chemical composition	292:311	chemical composition	292:311	Portulaca elatior is a Caatinga plant whose chemical composition and biotechnological potential have not been extensively studied.					
30583005	9	57	theme	Candida	1148:1154	arg1	albicans					1138:1145	Candida albicans	1130:1145	Candida albicans	1130:1145	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	9	57	theme	Candida	1148:1154	arg1	parapsilosis					1156:1167	Candida parapsilosis	1148:1167	Candida parapsilosis	1148:1167	PeRoL induced the death of Candida albicans, Candida parapsilosis, Candida krusei, and Candida tropicalis cells, with a minimal fungicidal concentration of 16 μg/mL.					
30583005	7	58	theme	present	886:892	arg1	Trehalose					825:833	Trehalose	825:833	Trehalose	825:833	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	7	58	theme	present	886:892	arg1	inhibitor					867:875	an endogenous inhibitor	853:875	an endogenous inhibitor of PeRoL present in the roots	853:905	Trehalose was identified as an endogenous inhibitor of PeRoL present in the roots.					
30583005	4	59	theme	15 kDa	604:609	arg1	subunits					592:599	two subunits	588:599	two subunits of 15 kDa linked by disulfide bonds	588:635	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	3	60	attach	isolated	406:413	arg1	extract					436:442	P. elatior root extract	420:442	P. elatior root extract	420:442	In this work, a lectin was isolated from P. elatior root extract and evaluated for antimicrobial activity.					
30583005	3	60	attach	isolated	406:413	arg2	lectin					395:400	a lectin	393:400	a lectin	393:400	In this work, a lectin was isolated from P. elatior root extract and evaluated for antimicrobial activity.					
30583005	0	61	with	lectin	52:57	arg1	activities					93:102	antibacterial and antifungal activities	64:102	activities	93:102	Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities.					
30583005	8	62	dep	detected	936:943	arg1	concentration					1045:1057	minimal inhibitory concentration	1026:1057	minimal inhibitory concentration of 8.1	1026:1064	Bacteriostatic activity was detected against Enterococcus faecalis, Pseudomonas aeruginosa and Staphylococcus aureus (minimal inhibitory concentration of 8.1, 32.5 and 4.06 μg/mL, respectively).					
30583005	4	63	theme	disulfide	621:629	arg1	bonds					631:635	disulfide bonds	621:635	disulfide bonds	621:635	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	0	64	theme	antibacterial	64:76	arg1	activities					93:102	antibacterial and antifungal activities	64:102	activities	93:102	Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities.					
30583005	4	65	theme	native	528:533	arg1	mass					545:548	native molecular mass	528:548	native molecular mass of 33 kDa, pI 3.8	528:566	The P. elatior root lectin (PeRoL) showed native molecular mass of 33 kDa, pI 3.8 and is comprised of two subunits of 15 kDa linked by disulfide bonds.					
30583005	3	66	theme	P.	420:421	arg1	extract					436:442	P. elatior root extract	420:442	P. elatior root extract	420:442	In this work, a lectin was isolated from P. elatior root extract and evaluated for antimicrobial activity.					
30583005	11	67	theme	antimicrobial	1485:1497	arg1	lectin					1499:1504	a trehalose-binding, thermostable, and antimicrobial lectin	1446:1504	a trehalose-binding, thermostable, and antimicrobial lectin	1446:1504	In conclusion, the roots of P. elatior contain a trehalose-binding, thermostable, and antimicrobial lectin.					
31826661	0	0	theme	quinoa	82:87	arg1	size					49:52	particle size	40:52	particle size	40:52	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	0	0	theme	quinoa	82:87	arg1	composition					67:77	chemical composition	58:77	chemical composition	58:77	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	7	1	theme	hammer-milled	897:909	arg1	fractions					911:919	hammer-milled fractions	897:919	hammer-milled fractions	897:919	For hammer-milled fractions, both the finest fractions resulted in breads with higher technological quality, as well as a final product with more fibre, minerals and proteins.					
31826661	4	2	theme	fractions	563:571	arg1	composition					542:552	the chemical composition	529:552	the chemical composition of these fractions	529:571	Results showed that the chemical composition of these fractions was very different and played a major role on bread quality.					
31826661	4	2	theme	fractions	563:571	arg1	different					582:590	different	582:590	different	582:590	Results showed that the chemical composition of these fractions was very different and played a major role on bread quality.					
31826661	6	3	theme	bread	814:818	arg1	volume					820:825	bread volume	814:825	bread volume	814:825	Re-milling quinoa and buckwheat fractions increased bread volume, although chemical composition still influenced bread properties.					
31826661	1	4	theme	quinoa	253:258	arg1	fractions					226:234	different fractions	216:234	different fractions of buckwheat and quinoa	216:258	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	0	5	theme	buckwheat	93:101	arg1	fractions					109:117	buckwheat flour fractions	93:117	buckwheat flour fractions	93:117	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	1	6	from	effect	154:159	arg1	behaviour					267:275	the behaviour	263:275	the behaviour of gluten-free dough and bread quality	263:314	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	5	7	theme	starch-rich	704:714	arg1	fractions					716:724	starch-rich fractions	704:724	starch-rich fractions	704:724	Proteins, lipids and fibre negatively affected bread quality, whereas starch-rich fractions were more adequate for breadmaking.					
31826661	3	8	dep	mill	468:471	arg1	obtain					476:481	obtain	476:481	to obtain samples of similar sizes	473:506	These fractions where then re-milled with a cyclonic mill to obtain samples of similar sizes.					
31826661	7	9	with	product	1021:1027	arg1	fibre					1039:1043	fibre	1039:1043	fibre	1039:1043	For hammer-milled fractions, both the finest fractions resulted in breads with higher technological quality, as well as a final product with more fibre, minerals and proteins.					
31826661	7	9	with	product	1021:1027	arg1	proteins					1059:1066	proteins	1059:1066	proteins	1059:1066	For hammer-milled fractions, both the finest fractions resulted in breads with higher technological quality, as well as a final product with more fibre, minerals and proteins.					
31826661	7	9	with	product	1021:1027	arg1	minerals					1046:1053	minerals	1046:1053	minerals	1046:1053	For hammer-milled fractions, both the finest fractions resulted in breads with higher technological quality, as well as a final product with more fibre, minerals and proteins.					
31826661	0	10	theme	fractions	109:117	arg1	size					49:52	particle size	40:52	particle size	40:52	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	0	10	theme	fractions	109:117	arg1	composition					67:77	chemical composition	58:77	chemical composition	58:77	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	4	11	theme	major	605:609	arg1	role					611:614	a major role	603:614	a major role	603:614	Results showed that the chemical composition of these fractions was very different and played a major role on bread quality.					
31826661	0	12	theme	flour	103:107	arg1	fractions					109:117	buckwheat flour fractions	93:117	buckwheat flour fractions	93:117	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	1	13	theme	physicochemical	164:178	arg1	properties					180:189	physicochemical properties	164:189	physicochemical properties	164:189	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	6	14	theme	quinoa	773:778	arg1	fractions					794:802	Re-milling quinoa and buckwheat fractions	762:802	Re-milling quinoa and buckwheat fractions	762:802	Re-milling quinoa and buckwheat fractions increased bread volume, although chemical composition still influenced bread properties.					
31826661	1	15	theme	properties	180:189	arg1	effect					154:159	the effect	150:159	the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality	150:314	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	0	16	theme	Gluten-free	0:10	arg1	breadmaking					12:22	Gluten-free breadmaking	0:22	Gluten-free breadmaking	0:22	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	4	17	theme	chemical	533:540	arg1	composition					542:552	the chemical composition	529:552	the chemical composition of these fractions	529:571	Results showed that the chemical composition of these fractions was very different and played a major role on bread quality.					
31826661	4	17	theme	chemical	533:540	arg1	different					582:590	different	582:590	different	582:590	Results showed that the chemical composition of these fractions was very different and played a major role on bread quality.					
31826661	6	18	theme	Re-milling	762:771	arg1	fractions					794:802	Re-milling quinoa and buckwheat fractions	762:802	Re-milling quinoa and buckwheat fractions	762:802	Re-milling quinoa and buckwheat fractions increased bread volume, although chemical composition still influenced bread properties.					
31826661	2	19	theme	hammer	364:369	arg1	mill					371:374	a hammer mill	362:374	a hammer mill	362:374	Quinoa and buckwheat grains were milled with a hammer mill and then separated in three fractions.					
31826661	7	20	theme	finest	931:936	arg1	fractions					938:946	both the finest fractions	922:946	both the finest fractions	922:946	For hammer-milled fractions, both the finest fractions resulted in breads with higher technological quality, as well as a final product with more fibre, minerals and proteins.					
31826661	6	21	theme	buckwheat	784:792	arg1	fractions					794:802	Re-milling quinoa and buckwheat fractions	762:802	Re-milling quinoa and buckwheat fractions	762:802	Re-milling quinoa and buckwheat fractions increased bread volume, although chemical composition still influenced bread properties.					
31826661	7	22	theme	higher	972:977	arg1	quality					993:999	higher technological quality	972:999	higher technological quality	972:999	For hammer-milled fractions, both the finest fractions resulted in breads with higher technological quality, as well as a final product with more fibre, minerals and proteins.					
31826661	1	23	theme	gluten-free	280:290	arg1	dough					292:296	gluten-free dough	280:296	gluten-free dough	280:296	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	5	24	theme	bread	681:685	arg1	quality					687:693	bread quality	681:693	bread quality	681:693	Proteins, lipids and fibre negatively affected bread quality, whereas starch-rich fractions were more adequate for breadmaking.					
31826661	6	25	theme	bread	875:879	arg1	properties					881:890	bread properties	875:890	bread properties	875:890	Re-milling quinoa and buckwheat fractions increased bread volume, although chemical composition still influenced bread properties.					
31826661	1	26	theme	particle	199:206	arg1	size					208:211	the particle size	195:211	the particle size of different fractions of buckwheat and quinoa	195:258	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	0	27	theme	particle	40:47	arg1	size					49:52	particle size	40:52	particle size	40:52	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	1	28	theme	dough	292:296	arg1	behaviour					267:275	the behaviour	263:275	the behaviour of gluten-free dough and bread quality	263:314	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	4	29	theme	bread	619:623	arg1	quality					625:631	bread quality	619:631	bread quality	619:631	Results showed that the chemical composition of these fractions was very different and played a major role on bread quality.					
31826661	1	30	theme	size	208:211	arg1	effect					154:159	the effect	150:159	the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality	150:314	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	7	31	with	breads	960:965	arg1	quality					993:999	higher technological quality	972:999	higher technological quality	972:999	For hammer-milled fractions, both the finest fractions resulted in breads with higher technological quality, as well as a final product with more fibre, minerals and proteins.					
31826661	0	32	dep	size	49:52	arg1	the					36:38	the	36:38	the	36:38	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	7	33	theme	final	1015:1019	arg1	product					1021:1027	a final product	1013:1027	a final product with more fibre, minerals and proteins	1013:1066	For hammer-milled fractions, both the finest fractions resulted in breads with higher technological quality, as well as a final product with more fibre, minerals and proteins.					
31826661	1	34	theme	bread	302:306	arg1	quality					308:314	bread quality	302:314	bread quality	302:314	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	1	35	theme	quality	308:314	arg1	behaviour					267:275	the behaviour	263:275	the behaviour of gluten-free dough and bread quality	263:314	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	6	36	theme	chemical	837:844	arg1	composition					846:856	chemical composition	837:856	chemical composition	837:856	Re-milling quinoa and buckwheat fractions increased bread volume, although chemical composition still influenced bread properties.					
31826661	2	37	theme	buckwheat	328:336	arg1	grains					338:343	Quinoa and buckwheat grains	317:343	Quinoa and buckwheat grains	317:343	Quinoa and buckwheat grains were milled with a hammer mill and then separated in three fractions.					
31826661	7	38	theme	technological	979:991	arg1	quality					993:999	higher technological quality	972:999	higher technological quality	972:999	For hammer-milled fractions, both the finest fractions resulted in breads with higher technological quality, as well as a final product with more fibre, minerals and proteins.					
31826661	3	39	theme	cyclonic	459:466	arg1	mill					468:471	a cyclonic mill	457:471	a cyclonic mill to obtain samples of similar sizes	457:506	These fractions where then re-milled with a cyclonic mill to obtain samples of similar sizes.					
31826661	1	40	theme	different	216:224	arg1	fractions					226:234	different fractions	216:234	different fractions of buckwheat and quinoa	216:258	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	2	41	theme	Quinoa	317:322	arg1	grains					338:343	Quinoa and buckwheat grains	317:343	Quinoa and buckwheat grains	317:343	Quinoa and buckwheat grains were milled with a hammer mill and then separated in three fractions.					
31826661	1	42	theme	fractions	226:234	arg1	properties					180:189	physicochemical properties	164:189	physicochemical properties	164:189	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	1	42	theme	fractions	226:234	arg1	size					208:211	the particle size	195:211	the particle size of different fractions of buckwheat and quinoa	195:258	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
31826661	3	43	theme	similar	494:500	arg1	sizes					502:506	similar sizes	494:506	similar sizes	494:506	These fractions where then re-milled with a cyclonic mill to obtain samples of similar sizes.					
31826661	0	44	theme	chemical	58:65	arg1	composition					67:77	chemical composition	58:77	chemical composition	58:77	Gluten-free breadmaking affected by the particle size and chemical composition of quinoa and buckwheat flour fractions.					
31826661	3	45	theme	sizes	502:506	arg1	samples					483:489	samples	483:489	samples of similar sizes	483:506	These fractions where then re-milled with a cyclonic mill to obtain samples of similar sizes.					
31826661	1	46	theme	buckwheat	239:247	arg1	fractions					226:234	different fractions	216:234	different fractions of buckwheat and quinoa	216:258	This study aimed at assessing the effect of physicochemical properties and the particle size of different fractions of buckwheat and quinoa on the behaviour of gluten-free dough and bread quality.					
29378089	9	0	theme	fourth	1790:1795	arg1	week					1797:1800	the third and fourth week	1776:1800	the third and fourth week of treatment	1776:1813	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	3	1	theme	derivatives	481:491	arg1	levels					442:447	the levels	438:447	the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol	438:536	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	10	2	theme	first	1958:1962	arg1	time					1964:1967	the first time	1954:1967	the first time in humans	1954:1977	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	7	3	theme	cholesterol	1173:1183	arg1	content					1185:1191	cholesterol content	1173:1191	cholesterol content	1173:1191	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	3	4	theme	infertile	549:557	arg1	patients					559:566	sixteen infertile patients	541:566	sixteen infertile patients with oligo-asthenozoospermia	541:595	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	4	5	theme	fertile	667:673	arg1	subjects					675:682	16 N fertile subjects	662:682	16 N fertile subjects	662:682	Furthermore, ten of 16 N fertile subjects agreed to receive a defined testicular thermal challenge by adhering to a programme of sauna sessions for 1 month.					
29378089	2	6	theme	infertile	246:254	arg1	patients					256:263	infertile patients	246:263	infertile patients	246:263	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	1	7	theme	potential	154:162	arg1	gain					136:139	the gain	132:139	the gain of fertility potential during sperm maturation	132:186	Membrane cholesterol removal appears a key step for the gain of fertility potential during sperm maturation.					
29378089	9	8	theme	7β-hydroxycholesterol	1823:1843	arg1	levels					1868:1873	7β-hydroxycholesterol and 7-keto-cholesterol levels	1823:1873	7β-hydroxycholesterol and 7-keto-cholesterol levels	1823:1873	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	1	9	theme	sperm	171:175	arg1	maturation					177:186	sperm maturation	171:186	sperm maturation	171:186	Membrane cholesterol removal appears a key step for the gain of fertility potential during sperm maturation.					
29378089	6	10	theme	7β-hydroxycholesterol	975:995	arg1	levels					952:957	Sperm levels	946:957	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol	946:1018	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol were quantified by ultra-pressure liquid chromatography mass spectrometry.					
29378089	9	11	theme	7-keto-cholesterol	1849:1866	arg1	levels					1868:1873	7β-hydroxycholesterol and 7-keto-cholesterol levels	1823:1873	7β-hydroxycholesterol and 7-keto-cholesterol levels	1823:1873	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	11	12	theme	strict	2107:2112	arg1	link					2126:2129	a strict biochemical link	2105:2129	a strict biochemical link relating testis function, sperm membrane status and male fertility potential	2105:2206	These findings suggest a strict biochemical link relating testis function, sperm membrane status and male fertility potential.					
29378089	8	13	theme	oxidized	1344:1351	arg1	derivatives					1353:1363	oxidized derivatives	1344:1363	oxidized derivatives	1344:1363	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	3	14	dep	derivatives	481:491	arg1	derivatives					481:491	its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol	468:536	its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol	468:536	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	3	14	dep	derivatives	481:491	arg1	7-keto-cholesterol					519:536	7-keto-cholesterol	519:536	7-keto-cholesterol	519:536	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	3	14	dep	derivatives	481:491	arg1	7β-hydroxycholesterol					493:513	7β-hydroxycholesterol	493:513	7β-hydroxycholesterol	493:513	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	2	15	theme	impaired	271:278	arg1	parameters					286:295	impaired sperm parameters	271:295	impaired sperm parameters	271:295	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	9	16	theme	sauna	1620:1624	arg1	exposure					1626:1633	sauna exposure	1620:1633	sauna exposure	1620:1633	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	6	17	theme	cholesterol	962:972	arg1	levels					952:957	Sperm levels	946:957	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol	946:1018	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol were quantified by ultra-pressure liquid chromatography mass spectrometry.					
29378089	2	18	theme	sterol	211:216	arg1	pattern					218:224	the membrane sterol pattern	198:224	the membrane sterol pattern	198:224	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	9	19	theme	earlier	1885:1891	arg1	enhancement					1893:1903	an earlier enhancement	1882:1903	an earlier enhancement starting from the second week	1882:1933	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	4	20	theme	thermal	723:729	arg1	challenge					731:739	a defined testicular thermal challenge	702:739	a defined testicular thermal challenge	702:739	Furthermore, ten of 16 N fertile subjects agreed to receive a defined testicular thermal challenge by adhering to a programme of sauna sessions for 1 month.					
29378089	3	21	theme	normozoospermic	604:618	arg1	N					621:621	N	621:621	N	621:621	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	3	21	theme	normozoospermic	604:618	arg1	subjects					632:639	16 normozoospermic (N) fertile subjects	601:639	16 normozoospermic (N) fertile subjects	601:639	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	5	22	theme	Health	916:921	arg1	criteria					936:943	the World Health Organization criteria	906:943	the World Health Organization criteria	906:943	Semen samples were obtained from each of the participants, and sperm parameters were assessed according to the World Health Organization criteria.					
29378089	2	23	from	patients	256:263	arg1	pattern					218:224	the membrane sterol pattern	198:224	the membrane sterol pattern	198:224	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	2	23	from	patients	256:263	arg1	cells					235:239	sperm cells	229:239	sperm cells from infertile patients	229:263	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	8	24	theme	oligo-asthenozoospermia	1394:1416	arg1	patients					1418:1425	oligo-asthenozoospermia patients	1394:1425	oligo-asthenozoospermia patients	1394:1425	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	7	25	contain	had	1152:1154	arg2	content					1185:1191	cholesterol content	1173:1191	cholesterol content	1173:1191	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	25	contain	had	1152:1154	arg2	amount					1163:1168	a huge amount	1156:1168	a huge amount of cholesterol content	1156:1191	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	25	contain	had	1152:1154	arg1	patients					1143:1150	oligo-asthenozoospermia patients	1119:1150	oligo-asthenozoospermia patients	1119:1150	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	10	26	theme	strong	1981:1986	arg1	difference					1988:1997	a strong difference	1979:1997	a strong difference in the cholesterol	1979:2016	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	6	27	theme	chromatography	1061:1074	arg1	spectrometry					1081:1092	ultra-pressure liquid chromatography mass spectrometry	1039:1092	ultra-pressure liquid chromatography mass spectrometry	1039:1092	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol were quantified by ultra-pressure liquid chromatography mass spectrometry.					
29378089	10	28	from	difference	1988:1997	arg1	cholesterol					2006:2016	the cholesterol	2002:2016	the cholesterol	2002:2016	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	3	29	theme	fertile	624:630	arg1	N					621:621	N	621:621	N	621:621	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	3	29	theme	fertile	624:630	arg1	subjects					632:639	16 normozoospermic (N) fertile subjects	601:639	16 normozoospermic (N) fertile subjects	601:639	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	6	30	theme	ultra-pressure	1039:1052	arg1	spectrometry					1081:1092	ultra-pressure liquid chromatography mass spectrometry	1039:1092	ultra-pressure liquid chromatography mass spectrometry	1039:1092	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol were quantified by ultra-pressure liquid chromatography mass spectrometry.					
29378089	0	31	from	function	15:22	arg1	men					37:39	infertile men	27:39	infertile men	27:39	Impaired sperm function in infertile men relies on the membrane sterol pattern.					
29378089	4	32	theme	defined	704:710	arg1	challenge					731:739	a defined testicular thermal challenge	702:739	a defined testicular thermal challenge	702:739	Furthermore, ten of 16 N fertile subjects agreed to receive a defined testicular thermal challenge by adhering to a programme of sauna sessions for 1 month.					
29378089	0	33	theme	sterol	64:69	arg1	pattern					71:77	the membrane sterol pattern	51:77	the membrane sterol pattern	51:77	Impaired sperm function in infertile men relies on the membrane sterol pattern.					
29378089	9	34	theme	sperm	1680:1684	arg1	parameters					1695:1704	sperm motility parameters	1680:1704	sperm motility parameters	1680:1704	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	4	35	theme	sauna	771:775	arg1	sessions					777:784	sauna sessions	771:784	sauna sessions	771:784	Furthermore, ten of 16 N fertile subjects agreed to receive a defined testicular thermal challenge by adhering to a programme of sauna sessions for 1 month.					
29378089	9	36	theme	parameters	1695:1704	arg1	worsening					1667:1675	a progressive worsening	1653:1675	a progressive worsening of sperm motility parameters	1653:1704	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	6	37	theme	7-keto-cholesterol	1001:1018	arg1	levels					952:957	Sperm levels	946:957	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol	946:1018	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol were quantified by ultra-pressure liquid chromatography mass spectrometry.					
29378089	8	38	theme	0.075 ± 0.05 ng/106	1480:1498	arg1	cells					1500:1504	0.075 ± 0.05 ng/106 cells	1480:1504	0.075 ± 0.05 ng/106 cells	1480:1504	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	2	39	from	pattern	218:224	arg1	cells					235:239	sperm cells	229:239	sperm cells from infertile patients	229:263	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	2	39	from	pattern	218:224	arg1	patients					256:263	infertile patients	246:263	infertile patients	246:263	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	7	40	dep	subjects	1215:1222	arg1	p < 0.001					1281:1289	p < 0.001	1281:1289	p < 0.001	1281:1289	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	40	dep	subjects	1215:1222	arg1	cells					1245:1249	12.40 ± 6.05 μg/106 cells	1225:1249	12.40 ± 6.05 μg/106 cells	1225:1249	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	40	dep	subjects	1215:1222	arg1	cells					1274:1278	0.45 ± 0.28 μg/106 cells	1255:1278	0.45 ± 0.28 μg/106 cells	1255:1278	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	40	dep	subjects	1215:1222	arg1	oligo-asthenozoospermia					1298:1320	oligo-asthenozoospermia	1298:1320	oligo-asthenozoospermia	1298:1320	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	40	dep	subjects	1215:1222	arg1	N					1292:1292	N	1292:1292	N	1292:1292	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	0	41	theme	Impaired	0:7	arg1	function					15:22	Impaired sperm function	0:22	Impaired sperm function in infertile men	0:39	Impaired sperm function in infertile men relies on the membrane sterol pattern.					
29378089	1	42	theme	Membrane	80:87	arg1	removal					101:107	Membrane cholesterol removal	80:107	Membrane cholesterol removal	80:107	Membrane cholesterol removal appears a key step for the gain of fertility potential during sperm maturation.					
29378089	3	43	theme	causative	343:351	arg1	link					353:356	a causative link	341:356	a causative link between sperm membrane composition in male fertility	341:409	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	9	44	theme	reversible	1729:1738	arg1	increase					1740:1747	a reversible increase	1727:1747	a reversible increase in sperm cholesterol	1727:1768	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	9	45	from	increase	1740:1747	arg1	cholesterol					1758:1768	sperm cholesterol	1752:1768	sperm cholesterol	1752:1768	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	7	46	theme	0.45 ± 0.28 μg/106	1255:1272	arg1	cells					1274:1278	0.45 ± 0.28 μg/106 cells	1255:1278	0.45 ± 0.28 μg/106 cells	1255:1278	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	0	47	theme	infertile	27:35	arg1	men					37:39	infertile men	27:39	infertile men	27:39	Impaired sperm function in infertile men relies on the membrane sterol pattern.					
29378089	8	48	dep	7β-hydroxycholesterol	1428:1448	arg1	p < 0.001					1598:1606	p < 0.001	1598:1606	p < 0.001	1598:1606	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	8	48	dep	7β-hydroxycholesterol	1428:1448	arg1	cells					1560:1564	1.11 ± 0.72 ng/106 cells	1541:1564	1.11 ± 0.72 ng/106 cells	1541:1564	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	8	48	dep	7β-hydroxycholesterol	1428:1448	arg1	cells					1500:1504	0.075 ± 0.05 ng/106 cells	1480:1504	0.075 ± 0.05 ng/106 cells	1480:1504	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	8	48	dep	7β-hydroxycholesterol	1428:1448	arg1	p < 0.001					1507:1515	p < 0.001	1507:1515	p < 0.001	1507:1515	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	8	48	dep	7β-hydroxycholesterol	1428:1448	arg1	cells					1591:1595	0.005 ± 0.003 ng/106 cells	1570:1595	0.005 ± 0.003 ng/106 cells	1570:1595	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	8	48	dep	7β-hydroxycholesterol	1428:1448	arg1	cells					1470:1474	1.96 ± 1.03 ng/106 cells	1451:1474	1.96 ± 1.03 ng/106 cells	1451:1474	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	8	48	dep	7β-hydroxycholesterol	1428:1448	arg1	7-keto-cholesterol					1521:1538	7-keto-cholesterol	1521:1538	7-keto-cholesterol	1521:1538	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	3	49	theme	membrane	372:379	arg1	composition					381:391	sperm membrane composition	366:391	sperm membrane composition in male fertility	366:409	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	7	50	theme	fertile	1207:1213	arg1	subjects					1215:1222	fertile subjects	1207:1222	fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively)	1207:1335	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	11	51	theme	testis	2140:2145	arg1	function					2147:2154	testis function	2140:2154	testis function	2140:2154	These findings suggest a strict biochemical link relating testis function, sperm membrane status and male fertility potential.					
29378089	8	52	theme	0.005 ± 0.003 ng/106	1570:1589	arg1	cells					1591:1595	0.005 ± 0.003 ng/106 cells	1570:1595	0.005 ± 0.003 ng/106 cells	1570:1595	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	11	53	theme	membrane	2163:2170	arg1	status					2172:2177	sperm membrane status	2157:2177	sperm membrane status	2157:2177	These findings suggest a strict biochemical link relating testis function, sperm membrane status and male fertility potential.					
29378089	5	54	theme	sperm	862:866	arg1	parameters					868:877	sperm parameters	862:877	sperm parameters	862:877	Semen samples were obtained from each of the participants, and sperm parameters were assessed according to the World Health Organization criteria.					
29378089	10	55	theme	fertile	2064:2070	arg1	subjects					2072:2079	infertile and fertile subjects	2050:2079	infertile and fertile subjects	2050:2079	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	7	56	theme	content	1185:1191	arg1	amount					1163:1168	a huge amount	1156:1168	a huge amount of cholesterol content	1156:1191	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	56	theme	content	1185:1191	arg1	content					1185:1191	cholesterol content	1173:1191	cholesterol content	1173:1191	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	3	57	theme	oxidized	472:479	arg1	derivatives					481:491	its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol	468:536	its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol	468:536	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	3	57	theme	oxidized	472:479	arg1	7-keto-cholesterol					519:536	7-keto-cholesterol	519:536	7-keto-cholesterol	519:536	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	3	57	theme	oxidized	472:479	arg1	7β-hydroxycholesterol					493:513	7β-hydroxycholesterol	493:513	7β-hydroxycholesterol	493:513	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	10	58	theme	infertile	2050:2058	arg1	subjects					2072:2079	infertile and fertile subjects	2050:2079	infertile and fertile subjects	2050:2079	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	9	59	theme	treatment	1805:1813	arg1	week					1797:1800	the third and fourth week	1776:1800	the third and fourth week of treatment	1776:1813	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	7	60	theme	huge	1158:1161	arg1	amount					1163:1168	a huge amount	1156:1168	a huge amount of cholesterol content	1156:1191	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	60	theme	huge	1158:1161	arg1	content					1185:1191	cholesterol content	1173:1191	cholesterol content	1173:1191	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	11	61	theme	sperm	2157:2161	arg1	status					2172:2177	sperm membrane status	2157:2177	sperm membrane status	2157:2177	These findings suggest a strict biochemical link relating testis function, sperm membrane status and male fertility potential.					
29378089	1	62	theme	fertility	144:152	arg1	potential					154:162	fertility potential	144:162	fertility potential	144:162	Membrane cholesterol removal appears a key step for the gain of fertility potential during sperm maturation.					
29378089	10	63	from	derivatives	2035:2045	arg1	cholesterol					2006:2016	the cholesterol	2002:2016	the cholesterol	2002:2016	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	4	64	theme	subjects	675:682	arg1	ten					655:657	ten	655:657	ten	655:657	Furthermore, ten of 16 N fertile subjects agreed to receive a defined testicular thermal challenge by adhering to a programme of sauna sessions for 1 month.					
29378089	4	64	theme	subjects	675:682	arg1	subjects					675:682	16 N fertile subjects	662:682	16 N fertile subjects	662:682	Furthermore, ten of 16 N fertile subjects agreed to receive a defined testicular thermal challenge by adhering to a programme of sauna sessions for 1 month.					
29378089	7	65	theme	oligo-asthenozoospermia	1119:1141	arg1	patients					1143:1150	oligo-asthenozoospermia patients	1119:1150	oligo-asthenozoospermia patients	1119:1150	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	2	66	theme	sperm	280:284	arg1	parameters					286:295	impaired sperm parameters	271:295	impaired sperm parameters	271:295	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	2	67	with	pattern	218:224	arg1	parameters					286:295	impaired sperm parameters	271:295	impaired sperm parameters	271:295	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	11	68	theme	biochemical	2114:2124	arg1	link					2126:2129	a strict biochemical link	2105:2129	a strict biochemical link relating testis function, sperm membrane status and male fertility potential	2105:2206	These findings suggest a strict biochemical link relating testis function, sperm membrane status and male fertility potential.					
29378089	2	69	theme	membrane	202:209	arg1	pattern					218:224	the membrane sterol pattern	198:224	the membrane sterol pattern	198:224	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	5	70	theme	World	910:914	arg1	criteria					936:943	the World Health Organization criteria	906:943	the World Health Organization criteria	906:943	Semen samples were obtained from each of the participants, and sperm parameters were assessed according to the World Health Organization criteria.					
29378089	6	71	theme	Sperm	946:950	arg1	levels					952:957	Sperm levels	946:957	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol	946:1018	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol were quantified by ultra-pressure liquid chromatography mass spectrometry.					
29378089	4	72	theme	testicular	712:721	arg1	challenge					731:739	a defined testicular thermal challenge	702:739	a defined testicular thermal challenge	702:739	Furthermore, ten of 16 N fertile subjects agreed to receive a defined testicular thermal challenge by adhering to a programme of sauna sessions for 1 month.					
29378089	1	73	theme	key	119:121	arg1	step					123:126	a key step	117:126	a key step for the gain of fertility potential during sperm maturation	117:186	Membrane cholesterol removal appears a key step for the gain of fertility potential during sperm maturation.					
29378089	11	74	theme	male	2183:2186	arg1	potential					2198:2206	male fertility potential	2183:2206	male fertility potential	2183:2206	These findings suggest a strict biochemical link relating testis function, sperm membrane status and male fertility potential.					
29378089	6	75	theme	mass	1076:1079	arg1	spectrometry					1081:1092	ultra-pressure liquid chromatography mass spectrometry	1039:1092	ultra-pressure liquid chromatography mass spectrometry	1039:1092	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol were quantified by ultra-pressure liquid chromatography mass spectrometry.					
29378089	0	76	theme	membrane	55:62	arg1	pattern					71:77	the membrane sterol pattern	51:77	the membrane sterol pattern	51:77	Impaired sperm function in infertile men relies on the membrane sterol pattern.					
29378089	2	77	theme	sperm	229:233	arg1	cells					235:239	sperm cells	229:239	sperm cells from infertile patients	229:263	However, the membrane sterol pattern in sperm cells from infertile patients, with impaired sperm parameters, has been poorly investigated.					
29378089	9	78	theme	progressive	1655:1665	arg1	worsening					1667:1675	a progressive worsening	1653:1675	a progressive worsening of sperm motility parameters	1653:1704	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	6	79	theme	liquid	1054:1059	arg1	spectrometry					1081:1092	ultra-pressure liquid chromatography mass spectrometry	1039:1092	ultra-pressure liquid chromatography mass spectrometry	1039:1092	Sperm levels of cholesterol, 7β-hydroxycholesterol and 7-keto-cholesterol were quantified by ultra-pressure liquid chromatography mass spectrometry.					
29378089	9	80	theme	second	1923:1928	arg1	week					1930:1933	the second week	1919:1933	the second week	1919:1933	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	3	81	with	subjects	632:639	arg1	oligo-asthenozoospermia					573:595	oligo-asthenozoospermia	573:595	oligo-asthenozoospermia	573:595	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	3	82	from	composition	381:391	arg1	fertility					401:409	male fertility	396:409	male fertility	396:409	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	9	83	theme	motility	1686:1693	arg1	parameters					1695:1704	sperm motility parameters	1680:1704	sperm motility parameters	1680:1704	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	11	84	theme	fertility	2188:2196	arg1	potential					2198:2206	male fertility potential	2183:2206	male fertility potential	2183:2206	These findings suggest a strict biochemical link relating testis function, sperm membrane status and male fertility potential.					
29378089	8	85	theme	1.96 ± 1.03 ng/106	1451:1468	arg1	cells					1470:1474	1.96 ± 1.03 ng/106 cells	1451:1474	1.96 ± 1.03 ng/106 cells	1451:1474	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	5	86	theme	Organization	923:934	arg1	criteria					936:943	the World Health Organization criteria	906:943	the World Health Organization criteria	906:943	Semen samples were obtained from each of the participants, and sperm parameters were assessed according to the World Health Organization criteria.					
29378089	4	87	theme	sessions	777:784	arg1	programme					758:766	a programme	756:766	a programme of sauna sessions for 1 month	756:796	Furthermore, ten of 16 N fertile subjects agreed to receive a defined testicular thermal challenge by adhering to a programme of sauna sessions for 1 month.					
29378089	0	88	theme	sperm	9:13	arg1	function					15:22	Impaired sperm function	0:22	Impaired sperm function in infertile men	0:39	Impaired sperm function in infertile men relies on the membrane sterol pattern.					
29378089	10	89	from	time	1964:1967	arg1	humans					1972:1977	humans	1972:1977	humans	1972:1977	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	3	90	theme	sperm	366:370	arg1	composition					381:391	sperm membrane composition	366:391	sperm membrane composition in male fertility	366:409	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	9	91	with	parallel	1639:1646	arg1	worsening					1667:1675	a progressive worsening	1653:1675	a progressive worsening of sperm motility parameters	1653:1704	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	7	92	theme	12.40 ± 6.05 μg/106	1225:1243	arg1	p < 0.001					1281:1289	p < 0.001	1281:1289	p < 0.001	1281:1289	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	92	theme	12.40 ± 6.05 μg/106	1225:1243	arg1	cells					1245:1249	12.40 ± 6.05 μg/106 cells	1225:1249	12.40 ± 6.05 μg/106 cells	1225:1249	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	92	theme	12.40 ± 6.05 μg/106	1225:1243	arg1	oligo-asthenozoospermia					1298:1320	oligo-asthenozoospermia	1298:1320	oligo-asthenozoospermia	1298:1320	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	7	92	theme	12.40 ± 6.05 μg/106	1225:1243	arg1	N					1292:1292	N	1292:1292	N	1292:1292	The results showed that oligo-asthenozoospermia patients had a huge amount of cholesterol content compared with fertile subjects (12.40 ± 6.05 μg/106 cells vs. 0.45 ± 0.28 μg/106 cells, p < 0.001, N and oligo-asthenozoospermia, respectively).					
29378089	8	93	dep	higher	1384:1389	arg1	7β-hydroxycholesterol					1428:1448	7β-hydroxycholesterol	1428:1448	7β-hydroxycholesterol	1428:1448	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	1	94	theme	cholesterol	89:99	arg1	removal					101:107	Membrane cholesterol removal	80:107	Membrane cholesterol removal	80:107	Membrane cholesterol removal appears a key step for the gain of fertility potential during sperm maturation.					
29378089	3	95	theme	male	396:399	arg1	fertility					401:409	male fertility	396:409	male fertility	396:409	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	5	96	theme	Semen	799:803	arg1	samples					805:811	Semen samples	799:811	Semen samples	799:811	Semen samples were obtained from each of the participants, and sperm parameters were assessed according to the World Health Organization criteria.					
29378089	9	97	theme	sperm	1752:1756	arg1	cholesterol					1758:1768	sperm cholesterol	1752:1768	sperm cholesterol	1752:1768	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	8	98	theme	1.11 ± 0.72 ng/106	1541:1558	arg1	cells					1560:1564	1.11 ± 0.72 ng/106 cells	1541:1564	1.11 ± 0.72 ng/106 cells	1541:1564	Also, oxidized derivatives were significantly higher in oligo-asthenozoospermia patients (7β-hydroxycholesterol: 1.96 ± 1.03 ng/106 cells vs. 0.075 ± 0.05 ng/106 cells, p < 0.001 and 7-keto-cholesterol: 1.11 ± 0.72 ng/106 cells vs. 0.005 ± 0.003 ng/106 cells, p < 0.001).					
29378089	10	99	theme	oxidized	2026:2033	arg1	derivatives					2035:2045	its oxidized derivatives	2022:2045	its oxidized derivatives of infertile and fertile subjects	2022:2079	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	3	100	theme	cholesterol	452:462	arg1	levels					442:447	the levels	438:447	the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol	438:536	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
29378089	9	101	theme	third	1780:1784	arg1	week					1797:1800	the third and fourth week	1776:1800	the third and fourth week of treatment	1776:1813	Moreover, sauna exposure, in parallel with a progressive worsening of sperm motility parameters, was associated with a reversible increase in sperm cholesterol after the third and fourth week of treatment, whilst 7β-hydroxycholesterol and 7-keto-cholesterol levels showed an earlier enhancement starting from the second week.					
29378089	10	102	theme	subjects	2072:2079	arg1	derivatives					2035:2045	its oxidized derivatives	2022:2045	its oxidized derivatives of infertile and fertile subjects	2022:2079	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	10	102	theme	subjects	2072:2079	arg1	difference					1988:1997	a strong difference	1979:1997	a strong difference in the cholesterol	1979:2016	Our data show for the first time in humans a strong difference in the cholesterol and its oxidized derivatives of infertile and fertile subjects.					
29378089	3	103	with	patients	559:566	arg1	oligo-asthenozoospermia					573:595	oligo-asthenozoospermia	573:595	oligo-asthenozoospermia	573:595	To elucidate a causative link between sperm membrane composition in male fertility, here we have investigated the levels of cholesterol and its oxidized derivatives 7β-hydroxycholesterol and 7-keto-cholesterol in sixteen infertile patients with oligo-asthenozoospermia and 16 normozoospermic (N) fertile subjects.					
31353715	8	0	dep	phenolics	1012:1020	arg1	100 g-1					1039:1045	69 mg GA 100 g-1	1030:1045	69 mg GA 100 g-1	1030:1045	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	8	0	dep	phenolics	1012:1020	arg1	66					1023:1024	66	1023:1024	66	1023:1024	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	6	1	theme	germinated	761:770	arg1	flours					772:777	germinated flours	761:777	germinated flours	761:777	Fiber content decreased around 73% after the extrusion of soaked and germinated flours.					
31353715	14	2	from	anti-nutrients	1815:1828	arg1	low					1808:1810	low	1808:1810	low	1808:1810	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	4	3	theme	fatty	553:557	arg1	acids					559:563	Polyunsaturated fatty acids	537:563	Polyunsaturated fatty acids	537:563	Polyunsaturated fatty acids increased after germination.					
31353715	5	4	theme	germinated	629:638	arg1	rice					640:643	germinated rice	629:643	germinated rice	629:643	Protein digestibility was 100% for germinated rice, decreased to 75% after the extrusion process.					
31353715	6	5	theme	soaked	750:755	arg1	extrusion					737:745	the extrusion	733:745	the extrusion of soaked and germinated flours	733:777	Fiber content decreased around 73% after the extrusion of soaked and germinated flours.					
31353715	7	6	theme	%	829:829	arg1	reduction					835:843	produces >50% IPs reduction	817:843	produces >50% IPs reduction	817:843	Soaking-extrusion combined processes produces >50% IPs reduction.					
31353715	5	7	theme	Protein	594:600	arg1	digestibility					602:614	Protein digestibility	594:614	Protein digestibility	594:614	Protein digestibility was 100% for germinated rice, decreased to 75% after the extrusion process.					
31353715	14	8	theme	pre-cooked	1886:1895	arg1	ingredients					1897:1907	pre-cooked ingredients	1886:1907	pre-cooked ingredients	1886:1907	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	1	9	theme	inositol	233:240	arg1	IPs					253:255	IPs	253:255	IPs	253:255	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	9	theme	inositol	233:240	arg1	phosphate					242:250	inositol phosphate	233:250	inositol phosphate (IPs)	233:256	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	3	10	theme	amino	525:529	arg1	acid					531:534	the limiting amino acid	512:534	the limiting amino acid	512:534	Lysine was the limiting amino acid.					
31353715	3	10	theme	amino	525:529	arg1	Lysine					501:506	Lysine	501:506	Lysine	501:506	Lysine was the limiting amino acid.					
31353715	14	11	theme	products	1874:1881	arg1	production					1851:1860	the production	1847:1860	the production of expanded products or pre-cooked ingredients	1847:1907	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	8	12	theme	highest	978:984	arg1	content					986:992	the highest content	974:992	the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively)	974:1061	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	9	13	theme	low	1135:1137	arg1	ingredients					1164:1174	low antinutrient whole grain ingredients	1135:1174	low antinutrient whole grain ingredients	1135:1174	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	1	14	theme	whole	316:320	arg1	ingredients					327:337	whole rice ingredients	316:337	whole rice ingredients processed by soaking, germination, and/or extrusion cooking	316:397	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	15	theme	available	172:180	arg1	lysine					182:187	available lysine	172:187	available lysine	172:187	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	13	16	theme	germinated	1693:1702	arg1	flours					1728:1733	germinated and germinated-extruded flours	1693:1733	germinated and germinated-extruded flours	1693:1733	We observed PA was highly reduced by soaking or germination combined with extrusion and antioxidant capacity and phenolic content increased in germinated and germinated-extruded flours compared to the other treatments.					
31353715	1	17	theme	ingredients	327:337	arg1	properties					147:156	nutritional properties	135:156	nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking	135:397	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	12	18	theme	flours	1531:1536	arg1	functionality					1514:1526	functionality	1514:1526	functionality	1514:1526	However, nutritional composition and functionality of flours can change.					
31353715	12	18	theme	flours	1531:1536	arg1	composition					1498:1508	composition	1498:1508	composition	1498:1508	However, nutritional composition and functionality of flours can change.					
31353715	13	19	theme	germinated-extruded	1708:1726	arg1	flours					1728:1733	germinated and germinated-extruded flours	1693:1733	germinated and germinated-extruded flours	1693:1733	We observed PA was highly reduced by soaking or germination combined with extrusion and antioxidant capacity and phenolic content increased in germinated and germinated-extruded flours compared to the other treatments.					
31353715	9	20	theme	whole	1208:1212	arg1	foods					1226:1230	whole grain-based foods	1208:1230	whole grain-based foods	1208:1230	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	9	21	theme	antinutrient	1139:1150	arg1	ingredients					1164:1174	low antinutrient whole grain ingredients	1135:1174	low antinutrient whole grain ingredients	1135:1174	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	10	22	dep	APPLICATIONS	1243:1254	arg1	antinutrient					1280:1291	an antinutrient	1277:1291	an antinutrient having negative effects on mineral bioavailability and protein digestibility	1277:1368	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	10	22	dep	APPLICATIONS	1243:1254	arg1	acid					1264:1267	Phytic acid	1257:1267	Phytic acid (PA)	1257:1272	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	1	23	theme	bound	268:272	arg1	phenolics					274:282	free and bound phenolics	259:282	free and bound phenolics	259:282	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	24	theme	nutritional	135:145	arg1	properties					147:156	nutritional properties	135:156	nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking	135:397	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	5	25	dep	decreased	646:654	arg1	%					623:623	100%	620:623	100% for germinated rice	620:643	Protein digestibility was 100% for germinated rice, decreased to 75% after the extrusion process.					
31353715	1	26	theme	extrusion	381:389	arg1	cooking					391:397	extrusion cooking	381:397	extrusion cooking	381:397	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	27	theme	antioxidant	289:299	arg1	properties					301:310	antioxidant properties	289:310	antioxidant properties	289:310	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	11	28	dep	or	1448:1449	arg1	soaking					1399:1405	soaking	1399:1405	soaking	1399:1405	There are processes such as soaking, germination, and extrusion, which alone or combined can decrease PA.					
31353715	11	28	dep	or	1448:1449	arg1	germination					1408:1418	germination	1408:1418	germination	1408:1418	There are processes such as soaking, germination, and extrusion, which alone or combined can decrease PA.					
31353715	11	28	dep	or	1448:1449	arg1	extrusion					1425:1433	extrusion	1425:1433	extrusion	1425:1433	There are processes such as soaking, germination, and extrusion, which alone or combined can decrease PA.					
31353715	8	29	theme	phenolics	1012:1020	arg1	content					986:992	the highest content	974:992	the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively)	974:1061	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	10	30	contain	having	1293:1298	arg1	antinutrient					1280:1291	an antinutrient	1277:1291	an antinutrient having negative effects on mineral bioavailability and protein digestibility	1277:1368	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	10	30	contain	having	1293:1298	arg2	effects					1309:1315	negative effects	1300:1315	negative effects on mineral bioavailability and protein digestibility	1300:1368	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	10	30	contain	having	1293:1298	arg1	acid					1264:1267	Phytic acid	1257:1267	Phytic acid (PA)	1257:1272	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	13	31	theme	antioxidant	1638:1648	arg1	capacity					1650:1657	antioxidant capacity	1638:1657	antioxidant capacity	1638:1657	We observed PA was highly reduced by soaking or germination combined with extrusion and antioxidant capacity and phenolic content increased in germinated and germinated-extruded flours compared to the other treatments.					
31353715	10	32	theme	PRACTICAL	1233:1241	arg1	APPLICATIONS					1243:1254	PRACTICAL APPLICATIONS	1233:1254	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.	1233:1369	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	14	33	theme	rice	1796:1799	arg1	flours					1801:1806	These modified whole grain rice flours	1769:1806	These modified whole grain rice flours low in anti-nutrients	1769:1828	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	0	34	theme	nutritional	16:26	arg1	properties					28:37	nutritional properties	16:37	nutritional properties of whole rice flours (Oryza sativa L.)	16:76	Modification of nutritional properties of whole rice flours (Oryza sativa L.) by soaking, germination, and extrusion.					
31353715	14	35	theme	whole	1784:1788	arg1	flours					1801:1806	These modified whole grain rice flours	1769:1806	These modified whole grain rice flours low in anti-nutrients	1769:1828	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	8	36	theme	69 mg	1030:1034	arg1	100 g-1					1039:1045	69 mg GA 100 g-1	1030:1045	69 mg GA 100 g-1	1030:1045	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	8	37	theme	highest	893:899	arg1	g-1					938:940	54 µmol trolox g-1	923:940	54 µmol trolox g-1	923:940	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	8	37	theme	highest	893:899	arg1	capacity					913:920	the highest antioxidant capacity	889:920	the highest antioxidant capacity (54 µmol trolox g-1 )	889:942	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	9	38	theme	Grain	1064:1068	arg1	treatments					1070:1079	Grain treatments	1064:1079	Grain treatments	1064:1079	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	14	39	theme	ingredients	1897:1907	arg1	production					1851:1860	the production	1847:1860	the production of expanded products or pre-cooked ingredients	1847:1907	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	1	40	dep	properties	147:156	arg1	acids					165:169	amino acids	159:169	amino acids	159:169	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	40	dep	properties	147:156	arg1	digestibility					198:210	protein digestibility	190:210	protein digestibility	190:210	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	40	dep	properties	147:156	arg1	properties					301:310	antioxidant properties	289:310	antioxidant properties	289:310	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	40	dep	properties	147:156	arg1	phosphate					242:250	inositol phosphate	233:250	inositol phosphate (IPs)	233:256	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	40	dep	properties	147:156	arg1	IPs					253:255	IPs	253:255	IPs	253:255	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	40	dep	properties	147:156	arg1	lysine					182:187	available lysine	172:187	available lysine	172:187	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	40	dep	properties	147:156	arg1	phenolics					274:282	free and bound phenolics	259:282	free and bound phenolics	259:282	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	40	dep	properties	147:156	arg1	acids					219:223	fatty acids	213:223	fatty acids	213:223	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	1	40	dep	properties	147:156	arg1	fiber					226:230	fiber	226:230	fiber	226:230	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	0	41	theme	rice	48:51	arg1	Oryza					61:65	Oryza	61:65	Oryza	61:65	Modification of nutritional properties of whole rice flours (Oryza sativa L.) by soaking, germination, and extrusion.					
31353715	0	41	theme	rice	48:51	arg1	flours					53:58	whole rice flours	42:58	whole rice flours (Oryza sativa L.)	42:76	Modification of nutritional properties of whole rice flours (Oryza sativa L.) by soaking, germination, and extrusion.					
31353715	1	42	theme	free	259:262	arg1	phenolics					274:282	free and bound phenolics	259:282	free and bound phenolics	259:282	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	14	43	theme	based	1932:1936	arg1	foods					1938:1942	whole grain based foods	1920:1942	whole grain based foods nutritionally improved	1920:1965	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	8	44	theme	54 µmol	923:929	arg1	g-1					938:940	54 µmol trolox g-1	923:940	54 µmol trolox g-1	923:940	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	8	44	theme	54 µmol	923:929	arg1	capacity					913:920	the highest antioxidant capacity	889:920	the highest antioxidant capacity (54 µmol trolox g-1 )	889:942	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	1	45	theme	protein	190:196	arg1	digestibility					198:210	protein digestibility	190:210	protein digestibility	190:210	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	14	46	theme	whole	1920:1924	arg1	foods					1938:1942	whole grain based foods	1920:1942	whole grain based foods nutritionally improved	1920:1965	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	9	47	theme	flours	1115:1120	arg1	properties					1101:1110	nutritional properties	1089:1110	nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods	1089:1230	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	10	48	theme	negative	1300:1307	arg1	effects					1309:1315	negative effects	1300:1315	negative effects on mineral bioavailability and protein digestibility	1300:1368	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	13	49	theme	other	1751:1755	arg1	treatments					1757:1766	the other treatments	1747:1766	the other treatments	1747:1766	We observed PA was highly reduced by soaking or germination combined with extrusion and antioxidant capacity and phenolic content increased in germinated and germinated-extruded flours compared to the other treatments.					
31353715	10	50	theme	protein	1348:1354	arg1	digestibility					1356:1368	protein digestibility	1348:1368	protein digestibility	1348:1368	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	4	51	theme	Polyunsaturated	537:551	arg1	acids					559:563	Polyunsaturated fatty acids	537:563	Polyunsaturated fatty acids	537:563	Polyunsaturated fatty acids increased after germination.					
31353715	9	52	theme	grain	1158:1162	arg1	ingredients					1164:1174	low antinutrient whole grain ingredients	1135:1174	low antinutrient whole grain ingredients	1135:1174	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	7	53	theme	IPs	831:833	arg1	reduction					835:843	produces >50% IPs reduction	817:843	produces >50% IPs reduction	817:843	Soaking-extrusion combined processes produces >50% IPs reduction.					
31353715	1	54	theme	properties	147:156	arg1	Modifications					118:130	Modifications	118:130	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking	118:397	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	3	55	theme	limiting	516:523	arg1	acid					531:534	the limiting amino acid	512:534	the limiting amino acid	512:534	Lysine was the limiting amino acid.					
31353715	3	55	theme	limiting	516:523	arg1	Lysine					501:506	Lysine	501:506	Lysine	501:506	Lysine was the limiting amino acid.					
31353715	7	56	theme	produces >50	817:828	arg1	%					829:829	%	829:829	%	829:829	Soaking-extrusion combined processes produces >50% IPs reduction.					
31353715	1	57	theme	amino	159:163	arg1	acids					165:169	amino acids	159:169	amino acids	159:169	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	8	58	with	flours	877:882	arg1	g-1					938:940	54 µmol trolox g-1	923:940	54 µmol trolox g-1	923:940	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	8	58	with	flours	877:882	arg1	capacity					913:920	the highest antioxidant capacity	889:920	the highest antioxidant capacity (54 µmol trolox g-1 )	889:942	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	14	59	theme	expanded	1865:1872	arg1	products					1874:1881	expanded products	1865:1881	expanded products	1865:1881	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	1	60	theme	rice	322:325	arg1	ingredients					327:337	whole rice ingredients	316:337	whole rice ingredients processed by soaking, germination, and/or extrusion cooking	316:397	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
31353715	9	61	theme	whole	1152:1156	arg1	ingredients					1164:1174	low antinutrient whole grain ingredients	1135:1174	low antinutrient whole grain ingredients	1135:1174	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	9	62	theme	grain-based	1214:1224	arg1	foods					1226:1230	whole grain-based foods	1208:1230	whole grain-based foods	1208:1230	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	8	63	theme	free	997:1000	arg1	phenolics					1012:1020	free and bound phenolics	997:1020	free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively)	997:1061	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	14	64	theme	low	1808:1810	arg1	flours					1801:1806	These modified whole grain rice flours	1769:1806	These modified whole grain rice flours low in anti-nutrients	1769:1828	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	13	65	theme	phenolic	1663:1670	arg1	content					1672:1678	phenolic content	1663:1678	phenolic content	1663:1678	We observed PA was highly reduced by soaking or germination combined with extrusion and antioxidant capacity and phenolic content increased in germinated and germinated-extruded flours compared to the other treatments.					
31353715	14	66	used	used	1839:1842	arg2	flours					1801:1806	These modified whole grain rice flours	1769:1806	These modified whole grain rice flours low in anti-nutrients	1769:1828	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	5	67	theme	extrusion	673:681	arg1	process					683:689	the extrusion process	669:689	the extrusion process	669:689	Protein digestibility was 100% for germinated rice, decreased to 75% after the extrusion process.					
31353715	8	68	theme	bound	1006:1010	arg1	phenolics					1012:1020	free and bound phenolics	997:1020	free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively)	997:1061	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	12	69	theme	nutritional	1486:1496	arg1	composition					1498:1508	composition	1498:1508	composition	1498:1508	However, nutritional composition and functionality of flours can change.					
31353715	6	70	theme	Fiber	692:696	arg1	content					698:704	Fiber content	692:704	Fiber content	692:704	Fiber content decreased around 73% after the extrusion of soaked and germinated flours.					
31353715	10	71	theme	Phytic	1257:1262	arg1	antinutrient					1280:1291	an antinutrient	1277:1291	an antinutrient having negative effects on mineral bioavailability and protein digestibility	1277:1368	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	10	71	theme	Phytic	1257:1262	arg1	PA					1270:1271	PA	1270:1271	PA	1270:1271	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	10	71	theme	Phytic	1257:1262	arg1	acid					1264:1267	Phytic acid	1257:1267	Phytic acid (PA)	1257:1272	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	0	72	theme	properties	28:37	arg1	Modification					0:11	Modification	0:11	Modification of nutritional properties of whole rice flours (Oryza sativa L.) by soaking, germination, and extrusion	0:115	Modification of nutritional properties of whole rice flours (Oryza sativa L.) by soaking, germination, and extrusion.					
31353715	14	73	theme	grain	1790:1794	arg1	flours					1801:1806	These modified whole grain rice flours	1769:1806	These modified whole grain rice flours low in anti-nutrients	1769:1828	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	8	74	theme	GA	1036:1037	arg1	100 g-1					1039:1045	69 mg GA 100 g-1	1030:1045	69 mg GA 100 g-1	1030:1045	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	0	75	dep	Oryza	61:65	arg1	L.					74:75	Oryza sativa L.	61:75	Oryza sativa L.	61:75	Modification of nutritional properties of whole rice flours (Oryza sativa L.) by soaking, germination, and extrusion.					
31353715	10	76	from	effects	1309:1315	arg1	bioavailability					1328:1342	mineral bioavailability	1320:1342	mineral bioavailability	1320:1342	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	10	76	from	effects	1309:1315	arg1	digestibility					1356:1368	protein digestibility	1348:1368	protein digestibility	1348:1368	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	0	77	theme	whole	42:46	arg1	Oryza					61:65	Oryza	61:65	Oryza	61:65	Modification of nutritional properties of whole rice flours (Oryza sativa L.) by soaking, germination, and extrusion.					
31353715	0	77	theme	whole	42:46	arg1	flours					53:58	whole rice flours	42:58	whole rice flours (Oryza sativa L.)	42:76	Modification of nutritional properties of whole rice flours (Oryza sativa L.) by soaking, germination, and extrusion.					
31353715	14	78	theme	modified	1775:1782	arg1	flours					1801:1806	These modified whole grain rice flours	1769:1806	These modified whole grain rice flours low in anti-nutrients	1769:1828	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	14	79	theme	improved	1958:1965	arg1	foods					1938:1942	whole grain based foods	1920:1942	whole grain based foods nutritionally improved	1920:1965	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	0	80	theme	flours	53:58	arg1	properties					28:37	nutritional properties	16:37	nutritional properties of whole rice flours (Oryza sativa L.)	16:76	Modification of nutritional properties of whole rice flours (Oryza sativa L.) by soaking, germination, and extrusion.					
31353715	8	81	theme	antioxidant	901:911	arg1	g-1					938:940	54 µmol trolox g-1	923:940	54 µmol trolox g-1	923:940	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	8	81	theme	antioxidant	901:911	arg1	capacity					913:920	the highest antioxidant capacity	889:920	the highest antioxidant capacity (54 µmol trolox g-1 )	889:942	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	9	82	theme	nutritional	1089:1099	arg1	properties					1101:1110	nutritional properties	1089:1110	nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods	1089:1230	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	9	83	used	used	1192:1195	arg2	ingredients					1164:1174	low antinutrient whole grain ingredients	1135:1174	low antinutrient whole grain ingredients	1135:1174	Grain treatments changed nutritional properties of flours resulting in low antinutrient whole grain ingredients, which could be used to develop whole grain-based foods.					
31353715	14	84	theme	grain	1926:1930	arg1	foods					1938:1942	whole grain based foods	1920:1942	whole grain based foods nutritionally improved	1920:1965	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	10	85	theme	mineral	1320:1326	arg1	bioavailability					1328:1342	mineral bioavailability	1320:1342	mineral bioavailability	1320:1342	PRACTICAL APPLICATIONS: Phytic acid (PA) is an antinutrient having negative effects on mineral bioavailability and protein digestibility.					
31353715	6	86	theme	flours	772:777	arg1	extrusion					737:745	the extrusion	733:745	the extrusion of soaked and germinated flours	733:777	Fiber content decreased around 73% after the extrusion of soaked and germinated flours.					
31353715	8	87	theme	trolox	931:936	arg1	g-1					938:940	54 µmol trolox g-1	923:940	54 µmol trolox g-1	923:940	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	8	87	theme	trolox	931:936	arg1	capacity					913:920	the highest antioxidant capacity	889:920	the highest antioxidant capacity (54 µmol trolox g-1 )	889:942	Germination-extrusion produced flours with the highest antioxidant capacity (54 µmol trolox g-1 ), which was in accordance with the highest content of free and bound phenolics (66 and 69 mg GA 100 g-1 , respectively).					
31353715	14	88	from	low	1808:1810	arg1	anti-nutrients					1815:1828	anti-nutrients	1815:1828	anti-nutrients	1815:1828	These modified whole grain rice flours low in anti-nutrients could be used in the production of expanded products or pre-cooked ingredients to develop whole grain based foods nutritionally improved.					
31353715	1	89	theme	fatty	213:217	arg1	acids					219:223	fatty acids	213:223	fatty acids	213:223	Modifications of nutritional properties (amino acids, available lysine, protein digestibility, fatty acids, fiber, inositol phosphate (IPs), free and bound phenolics, and antioxidant properties) of whole rice ingredients processed by soaking, germination, and/or extrusion cooking were evaluated.					
30785201	6	0	from	small	1326:1330	arg1	contrast					1162:1169	contrast	1162:1169	contrast	1162:1169	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	4	1	theme	N.	844:845	arg1	sylvestris					847:856	N. sylvestris	844:856	N. sylvestris	844:856	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	6	2	theme	13C	1219:1221	arg1	fractionations					1223:1236	post-photosynthetic 13C fractionations	1199:1236	post-photosynthetic 13C fractionations	1199:1236	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	1	3	theme	photosynthetic	167:180	arg1	fixation					186:193	photosynthetic CO2 fixation	167:193	photosynthetic CO2 fixation	167:193	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	6	4	from	contrast	1162:1169	arg1	effect					1189:1194	the PGM-knockout effect	1172:1194	the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration	1172:1320	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	6	4	from	contrast	1162:1169	arg1	small					1326:1330	small	1326:1330	small	1326:1330	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	7	5	theme	photosynthetic	1446:1459	arg1	fractionations					1465:1478	photosynthetic 13C fractionations	1446:1478	photosynthetic 13C fractionations	1446:1478	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	4	6	from	δ13CR	886:890	arg1	plants					1027:1032	wild-type plants	1017:1032	wild-type plants during a diel cycle	1017:1052	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	1	7	theme	CO2	182:184	arg1	fixation					186:193	photosynthetic CO2 fixation	167:193	photosynthetic CO2 fixation	167:193	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	6	8	theme	post-photosynthetic	1199:1217	arg1	fractionations					1223:1236	post-photosynthetic 13C fractionations	1199:1236	post-photosynthetic 13C fractionations	1199:1236	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	4	9	theme	pgm-induced	798:808	arg1	deficiency					817:826	the pgm-induced starch deficiency	794:826	the pgm-induced starch deficiency	794:826	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	0	10	from	Influence	0:8	arg1	fractionations					88:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.					
30785201	2	11	from	variation	324:332	arg1	metabolism					344:353	starch metabolism	337:353	starch metabolism in the leaf	337:365	However, responses of 13C fractionations to diel variation in starch metabolism in the leaf are not fully understood.					
30785201	3	12	theme	CO2	526:528	arg1	δ13C					462:465	δ13C	462:465	δ13C	462:465	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	12	theme	CO2	526:528	arg1	concentrations					443:456	concentrations	443:456	concentrations	443:456	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	12	theme	CO2	526:528	arg1	exchange					547:554	gas exchange	543:554	gas exchange	543:554	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	12	theme	CO2	526:528	arg1	δ13C					504:507	δ13C	504:507	δ13C of dark-respired CO2 (δ13CR)	504:536	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	12	theme	CO2	526:528	arg1	δ13C					410:413	δ13C	410:413	δ13C of organic matter (δ13COM)	410:440	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	1	13	theme	13C	120:122	arg1	fractionations					125:138	Carbon isotope (13C) fractionations	104:138	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation	104:193	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	4	14	from	values	902:907	arg1	plants					1027:1032	wild-type plants	1017:1032	wild-type plants during a diel cycle	1017:1052	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	2	15	theme	diel	319:322	arg1	variation					324:332	diel variation	319:332	diel variation in starch metabolism in the leaf	319:365	However, responses of 13C fractionations to diel variation in starch metabolism in the leaf are not fully understood.					
30785201	7	16	from	variations	1380:1389	arg1	mutants					1411:1417	starch-deficient mutants	1394:1417	starch-deficient mutants	1394:1417	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	5	17	from	differences	1068:1078	arg1	values					1088:1093	δ13C values	1083:1093	δ13C values	1083:1093	The genotype differences in δ13C values could be largely explained by differences in leaf gas exchange.					
30785201	2	18	from	metabolism	344:353	arg1	leaf					362:365	the leaf	358:365	the leaf	358:365	However, responses of 13C fractionations to diel variation in starch metabolism in the leaf are not fully understood.					
30785201	3	19	theme	respiratory	480:490	arg1	substrates					492:501	potential respiratory substrates	470:501	potential respiratory substrates	470:501	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	5	20	from	differences	1125:1135	arg1	exchange					1149:1156	leaf gas exchange	1140:1156	leaf gas exchange	1140:1156	The genotype differences in δ13C values could be largely explained by differences in leaf gas exchange.					
30785201	3	21	dep	Pisum	747:751	arg1	sativum					753:759	Pisum sativum	747:759	Pisum sativum	747:759	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	4	22	dep	malate	974:979	arg1	i.e.					969:972	i.e.	969:972	i.e.	969:972	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	4	23	theme	diel	1043:1046	arg1	cycle					1048:1052	a diel cycle	1041:1052	a diel cycle	1041:1052	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	3	24	theme	matter	426:431	arg1	δ13C					462:465	δ13C	462:465	δ13C	462:465	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	24	theme	matter	426:431	arg1	concentrations					443:456	concentrations	443:456	concentrations	443:456	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	24	theme	matter	426:431	arg1	exchange					547:554	gas exchange	543:554	gas exchange	543:554	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	24	theme	matter	426:431	arg1	δ13C					504:507	δ13C	504:507	δ13C of dark-respired CO2 (δ13CR)	504:536	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	24	theme	matter	426:431	arg1	δ13C					410:413	δ13C	410:413	δ13C of organic matter (δ13COM)	410:440	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	4	25	theme	starch	810:815	arg1	deficiency					817:826	the pgm-induced starch deficiency	794:826	the pgm-induced starch deficiency	794:826	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	7	26	theme	isotope	1529:1535	arg1	analyses					1537:1544	isotope analyses	1529:1544	isotope analyses	1529:1544	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	3	27	theme	dark-respired	512:524	arg1	CO2					526:528	dark-respired CO2	512:528	dark-respired CO2 (δ13CR)	512:536	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	27	theme	dark-respired	512:524	arg1	δ13CR					531:535	δ13CR	531:535	δ13CR	531:535	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	7	28	theme	plant	1578:1582	arg1	metabolism					1584:1593	plant metabolism	1578:1593	plant metabolism	1578:1593	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	3	29	dep	Arabidopsis	668:678	arg1	thaliana					680:687	Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum	668:759	thaliana	680:687	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	1	30	theme	Carbon	104:109	arg1	fractionations					125:138	Carbon isotope (13C) fractionations	104:138	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation	104:193	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	6	31	theme	fructose-1,6-bisphosphate	1256:1280	arg1	reaction					1291:1298	the plastidic fructose-1,6-bisphosphate aldolase reaction	1242:1298	the plastidic fructose-1,6-bisphosphate aldolase reaction	1242:1298	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	7	32	theme	δ13C	1375:1378	arg1	variations					1380:1389	the δ13C variations	1371:1389	the δ13C variations in starch-deficient mutants	1371:1417	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	4	33	theme	organic	954:960	arg1	acids					962:966	organic acids	954:966	organic acids (i.e. malate and citrate)	954:992	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	1	34	theme	isotope	111:117	arg1	fractionations					125:138	Carbon isotope (13C) fractionations	104:138	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation	104:193	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	1	35	theme	carbon	205:210	arg1	composition					220:230	the carbon isotope composition	201:230	the carbon isotope composition (δ13C) of plant material	201:255	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	1	35	theme	carbon	205:210	arg1	δ13C					233:236	δ13C	233:236	δ13C	233:236	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	0	36	theme	deficiency	20:29	arg1	Influence					0:8	Influence	0:8	Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.	0:102	Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.					
30785201	4	37	dep	assimilates	913:923	arg1	starch					942:947	starch	942:947	starch	942:947	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	4	37	dep	assimilates	913:923	arg1	sugars					931:936	sugars	931:936	sugars	931:936	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	2	38	theme	fractionations	301:314	arg1	responses					284:292	responses	284:292	responses of 13C fractionations to diel variation in starch metabolism in the leaf	284:365	However, responses of 13C fractionations to diel variation in starch metabolism in the leaf are not fully understood.					
30785201	4	39	theme	wild-type	1017:1025	arg1	plants					1027:1032	wild-type plants	1017:1032	wild-type plants during a diel cycle	1017:1052	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	1	40	theme	isotope	212:218	arg1	composition					220:230	the carbon isotope composition	201:230	the carbon isotope composition (δ13C) of plant material	201:255	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	1	40	theme	isotope	212:218	arg1	δ13C					233:236	δ13C	233:236	δ13C	233:236	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	0	41	theme	starch	13:18	arg1	deficiency					20:29	starch deficiency	13:29	starch deficiency	13:29	Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.					
30785201	7	42	theme	analyses	1537:1544	arg1	combination					1493:1503	the combination	1489:1503	the combination of knockout mutants and isotope analyses	1489:1544	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	6	43	theme	aldolase	1282:1289	arg1	reaction					1291:1298	the plastidic fructose-1,6-bisphosphate aldolase reaction	1242:1298	the plastidic fructose-1,6-bisphosphate aldolase reaction	1242:1298	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	2	44	theme	13C	297:299	arg1	fractionations					301:314	13C fractionations	297:314	13C fractionations	297:314	However, responses of 13C fractionations to diel variation in starch metabolism in the leaf are not fully understood.					
30785201	0	45	theme	photosynthetic	34:47	arg1	fractionations					88:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.					
30785201	3	46	theme	Nicotiana	721:729	arg1	sylvestris					731:740	Nicotiana sylvestris	721:740	Nicotiana sylvestris	721:740	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	46	theme	Nicotiana	721:729	arg1	species					659:665	four species	654:665	four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum)	654:760	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	4	47	theme	δ13C	897:900	arg1	values					902:907	δ13C values	897:907	δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle	897:1052	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	7	48	theme	knockout	1508:1515	arg1	mutants					1517:1523	knockout mutants	1508:1523	knockout mutants	1508:1523	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	3	49	theme	organic	418:424	arg1	matter					426:431	organic matter	418:431	organic matter (δ13COM)	418:440	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	49	theme	organic	418:424	arg1	δ13COM					434:439	δ13COM	434:439	δ13COM	434:439	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	5	50	theme	genotype	1059:1066	arg1	differences					1068:1078	The genotype differences	1055:1078	The genotype differences in δ13C values	1055:1093	The genotype differences in δ13C values could be largely explained by differences in leaf gas exchange.					
30785201	6	51	theme	plastidic	1246:1254	arg1	reaction					1291:1298	the plastidic fructose-1,6-bisphosphate aldolase reaction	1242:1298	the plastidic fructose-1,6-bisphosphate aldolase reaction	1242:1298	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	4	52	located	observed	832:839	arg1	sylvestris					847:856	N. sylvestris	844:856	N. sylvestris	844:856	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	4	52	located	observed	832:839	arg2	response					782:789	The strongest δ13C response	763:789	The strongest δ13C response to the pgm-induced starch deficiency	763:826	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	7	53	theme	starch-deficient	1394:1409	arg1	mutants					1411:1417	starch-deficient mutants	1394:1417	starch-deficient mutants	1394:1417	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	5	54	theme	δ13C	1083:1086	arg1	values					1088:1093	δ13C values	1083:1093	δ13C values	1083:1093	The genotype differences in δ13C values could be largely explained by differences in leaf gas exchange.					
30785201	0	55	theme	post-photosynthetic	53:71	arg1	fractionations					88:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.					
30785201	3	56	theme	pgm	617:619	arg1	mutants					622:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	57	theme	gas	543:545	arg1	exchange					547:554	gas exchange	543:554	gas exchange	543:554	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	7	58	theme	mutants	1517:1523	arg1	combination					1493:1503	the combination	1489:1503	the combination of knockout mutants and isotope analyses	1489:1544	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	3	59	theme	species	659:665	arg1	mutants					622:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	59	theme	species	659:665	arg1	plants					644:649	wild-type plants	634:649	wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum)	634:760	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	6	60	theme	PGM-knockout	1176:1187	arg1	effect					1189:1194	the PGM-knockout effect	1172:1194	the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration	1172:1320	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	6	60	theme	PGM-knockout	1176:1187	arg1	small					1326:1330	small	1326:1330	small	1326:1330	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	4	61	dep	sugars	931:936	arg1	i.e.					926:929	i.e.	926:929	i.e.	926:929	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	4	62	from	δ13COM	878:883	arg1	plants					1027:1032	wild-type plants	1017:1032	wild-type plants during a diel cycle	1017:1052	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	3	63	theme	substrates	492:501	arg1	δ13C					462:465	δ13C	462:465	δ13C	462:465	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	63	theme	substrates	492:501	arg1	concentrations					443:456	concentrations	443:456	concentrations	443:456	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	63	theme	substrates	492:501	arg1	exchange					547:554	gas exchange	543:554	gas exchange	543:554	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	63	theme	substrates	492:501	arg1	δ13C					504:507	δ13C	504:507	δ13C of dark-respired CO2 (δ13CR)	504:536	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	3	63	theme	substrates	492:501	arg1	δ13C					410:413	δ13C	410:413	δ13C of organic matter (δ13COM)	410:440	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	2	64	theme	starch	337:342	arg1	metabolism					344:353	starch metabolism	337:353	starch metabolism in the leaf	337:365	However, responses of 13C fractionations to diel variation in starch metabolism in the leaf are not fully understood.					
30785201	4	65	theme	strongest	767:775	arg1	response					782:789	The strongest δ13C response	763:789	The strongest δ13C response to the pgm-induced starch deficiency	763:826	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	3	66	theme	plastidial	586:595	arg1	mutants					622:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	4	67	theme	pgm	997:999	arg1	mutants					1001:1007	pgm mutants	997:1007	pgm mutants	997:1007	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	5	68	theme	leaf	1140:1143	arg1	exchange					1149:1156	leaf gas exchange	1140:1156	leaf gas exchange	1140:1156	The genotype differences in δ13C values could be largely explained by differences in leaf gas exchange.					
30785201	4	69	theme	negative	869:876	arg1	δ13COM					878:883	more negative δ13COM	864:883	more negative δ13COM	864:883	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	7	70	theme	13C	1461:1463	arg1	fractionations					1465:1478	photosynthetic 13C fractionations	1446:1478	photosynthetic 13C fractionations	1446:1478	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	3	71	theme	phosphoglucomutase	597:614	arg1	mutants					622:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	5	72	theme	gas	1145:1147	arg1	exchange					1149:1156	leaf gas exchange	1140:1156	leaf gas exchange	1140:1156	The genotype differences in δ13C values could be largely explained by differences in leaf gas exchange.					
30785201	0	73	theme	isotope	80:86	arg1	fractionations					88:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.					
30785201	3	74	theme	wild-type	634:642	arg1	plants					644:649	wild-type plants	634:649	wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum)	634:760	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	4	75	theme	δ13C	777:780	arg1	response					782:789	The strongest δ13C response	763:789	The strongest δ13C response to the pgm-induced starch deficiency	763:826	The strongest δ13C response to the pgm-induced starch deficiency was observed in N. sylvestris, with more negative δ13COM, δ13CR, and δ13C values for assimilates (i.e. sugars and starch) and organic acids (i.e. malate and citrate) in pgm mutants than in wild-type plants during a diel cycle.					
30785201	7	76	theme	additional	1553:1562	arg1	insights					1564:1571	additional insights	1553:1571	additional insights into plant metabolism	1553:1593	Taken together, our results show that the δ13C variations in starch-deficient mutants are primarily explained by photosynthetic 13C fractionations and that the combination of knockout mutants and isotope analyses allows additional insights into plant metabolism.					
30785201	1	77	theme	plant	242:246	arg1	material					248:255	plant material	242:255	plant material	242:255	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	0	78	theme	carbon	73:78	arg1	fractionations					88:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	photosynthetic and post-photosynthetic carbon isotope fractionations	34:101	Influence of starch deficiency on photosynthetic and post-photosynthetic carbon isotope fractionations.					
30785201	3	79	theme	starch-deficient	569:584	arg1	mutants					622:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	starch-deficient plastidial phosphoglucomutase (pgm) mutants	569:628	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	6	80	from	effect	1189:1194	arg1	fractionations					1223:1236	post-photosynthetic 13C fractionations	1199:1236	post-photosynthetic 13C fractionations	1199:1236	In contrast, the PGM-knockout effect on post-photosynthetic 13C fractionations via the plastidic fructose-1,6-bisphosphate aldolase reaction or during respiration was small.					
30785201	3	81	theme	potential	470:478	arg1	substrates					492:501	potential respiratory substrates	470:501	potential respiratory substrates	470:501	Here we measured δ13C of organic matter (δ13COM), concentrations and δ13C of potential respiratory substrates, δ13C of dark-respired CO2 (δ13CR), and gas exchange in leaves of starch-deficient plastidial phosphoglucomutase (pgm) mutants and wild-type plants of four species (Arabidopsis thaliana, Mesembryanthemum crystallinum, Nicotiana sylvestris, and Pisum sativum).					
30785201	1	82	theme	material	248:255	arg1	composition					220:230	the carbon isotope composition	201:230	the carbon isotope composition (δ13C) of plant material	201:255	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30785201	1	82	theme	material	248:255	arg1	δ13C					233:236	δ13C	233:236	δ13C	233:236	Carbon isotope (13C) fractionations occurring during and after photosynthetic CO2 fixation shape the carbon isotope composition (δ13C) of plant material and respired CO2.					
30703318	0	0	theme	Transdermal	90:100	arg1	Delivery					107:114	Controllable Transdermal Drug Delivery	77:114	Controllable Transdermal Drug Delivery	77:114	Highly Porous Silk Fibroin Scaffold Packed in PEGDA/Sucrose Microneedles for Controllable Transdermal Drug Delivery.					
30703318	5	1	theme	effective	1161:1169	arg1	delivery					1183:1190	effective transdermal delivery	1161:1190	effective transdermal delivery	1161:1190	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	6	2	theme	polymeric	1350:1358	arg1	microneedles					1360:1371	polymeric microneedles	1350:1371	polymeric microneedles toward transdermal drug delivery applications	1350:1417	The presented method provides a simple strategy for quick fabrication of polymeric microneedles toward transdermal drug delivery applications.					
30703318	2	3	theme	vacuum	421:426	arg1	processes					438:446	vacuum degassing processes	421:446	vacuum degassing processes	421:446	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	4	4	theme	photoinduced	964:975	arg1	polymerization					977:990	photoinduced polymerization	964:990	photoinduced polymerization	964:990	Fibroin scaffolds can instantly adsorb prepolymer solution due to capillary force, and subsequently initiate the formation of microneedles via photoinduced polymerization.					
30703318	0	5	theme	Controllable	77:88	arg1	Delivery					107:114	Controllable Transdermal Drug Delivery	77:114	Controllable Transdermal Drug Delivery	77:114	Highly Porous Silk Fibroin Scaffold Packed in PEGDA/Sucrose Microneedles for Controllable Transdermal Drug Delivery.					
30703318	5	6	theme	transdermal	1171:1181	arg1	delivery					1183:1190	effective transdermal delivery	1161:1190	effective transdermal delivery	1161:1190	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	3	7	theme	negative	578:585	arg1	mold					587:590	a novel polydimethylsiloxane (PDMS)-based negative mold	536:590	a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold	536:638	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	2	8	with	molds	341:345	arg1	cavities					366:373	needle-shaped cavities	352:373	needle-shaped cavities	352:373	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	3	9	with	mold	587:590	arg1	cavities					597:604	cavities	597:604	cavities packed with silk fibroin scaffold	597:638	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	2	10	theme	negative	332:339	arg1	molds					341:345	negative molds	332:345	negative molds with needle-shaped cavities	332:373	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	0	11	theme	Drug	102:105	arg1	Delivery					107:114	Controllable Transdermal Drug Delivery	77:114	Controllable Transdermal Drug Delivery	77:114	Highly Porous Silk Fibroin Scaffold Packed in PEGDA/Sucrose Microneedles for Controllable Transdermal Drug Delivery.					
30703318	5	12	theme	fabricated	1111:1120	arg1	microneedles					1136:1147	the fabricated PEGDA/sucrose microneedles	1107:1147	the fabricated PEGDA/sucrose microneedles	1107:1147	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	5	13	theme	Rhodamine	1040:1048	arg1	RhB					1053:1055	RhB	1053:1055	RhB	1053:1055	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	5	13	theme	Rhodamine	1040:1048	arg1	B					1050:1050	Rhodamine B	1040:1050	Rhodamine B (RhB)	1040:1056	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	6	14	theme	quick	1329:1333	arg1	fabrication					1335:1345	quick fabrication	1329:1345	quick fabrication of polymeric microneedles toward transdermal drug delivery applications	1329:1417	The presented method provides a simple strategy for quick fabrication of polymeric microneedles toward transdermal drug delivery applications.					
30703318	3	15	theme	silk	618:621	arg1	scaffold					631:638	silk fibroin scaffold	618:638	silk fibroin scaffold	618:638	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	6	16	theme	drug	1392:1395	arg1	applications					1406:1417	transdermal drug delivery applications	1380:1417	transdermal drug delivery applications	1380:1417	The presented method provides a simple strategy for quick fabrication of polymeric microneedles toward transdermal drug delivery applications.					
30703318	3	17	theme	sucrose	791:797	arg1	composition					731:741	the composition	727:741	the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix	727:818	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	3	18	theme	novel	538:542	arg1	mold					587:590	a novel polydimethylsiloxane (PDMS)-based negative mold	536:590	a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold	536:638	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	1	19	theme	invasive	191:198	arg1	platform					200:207	a minimally invasive platform	179:207	a minimally invasive platform for delivering drugs or vaccines in a more patient-friendly manner	179:274	Polymeric microneedles have attracted increasing attention as a minimally invasive platform for delivering drugs or vaccines in a more patient-friendly manner.					
30703318	1	19	theme	invasive	191:198	arg1	attention					166:174	increasing attention	155:174	increasing attention	155:174	Polymeric microneedles have attracted increasing attention as a minimally invasive platform for delivering drugs or vaccines in a more patient-friendly manner.					
30703318	2	20	theme	microfabrication	298:313	arg1	techniques					315:324	traditional microfabrication techniques	286:324	traditional microfabrication techniques using negative molds with needle-shaped cavities	286:373	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	0	21	theme	Porous	7:12	arg1	Scaffold					27:34	Highly Porous Silk Fibroin Scaffold	0:34	Highly Porous Silk Fibroin Scaffold	0:34	Highly Porous Silk Fibroin Scaffold Packed in PEGDA/Sucrose Microneedles for Controllable Transdermal Drug Delivery.					
30703318	6	22	theme	simple	1309:1314	arg1	strategy					1316:1323	a simple strategy	1307:1323	a simple strategy for quick fabrication of polymeric microneedles toward transdermal drug delivery applications	1307:1417	The presented method provides a simple strategy for quick fabrication of polymeric microneedles toward transdermal drug delivery applications.					
30703318	5	23	theme	therapeutic	1220:1230	arg1	molecules					1232:1240	therapeutic molecules	1220:1240	therapeutic molecules	1220:1240	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	5	24	theme	PEGDA/sucrose	1122:1134	arg1	microneedles					1136:1147	the fabricated PEGDA/sucrose microneedles	1107:1147	the fabricated PEGDA/sucrose microneedles	1107:1147	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	2	25	theme	microneedles	514:525	arg1	production					490:499	the scaled-up mass production	471:499	the scaled-up mass production of polymeric microneedles	471:525	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	3	26	theme	needle	806:811	arg1	matrix					813:818	the needle matrix	802:818	the needle matrix	802:818	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	2	27	theme	needle-shaped	352:364	arg1	cavities					366:373	needle-shaped cavities	352:373	needle-shaped cavities	352:373	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	0	28	theme	Fibroin	19:25	arg1	Scaffold					27:34	Highly Porous Silk Fibroin Scaffold	0:34	Highly Porous Silk Fibroin Scaffold	0:34	Highly Porous Silk Fibroin Scaffold Packed in PEGDA/Sucrose Microneedles for Controllable Transdermal Drug Delivery.					
30703318	5	29	theme	molecules	1232:1240	arg1	release					1209:1215	controllable release	1196:1215	controllable release	1196:1215	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	5	29	theme	molecules	1232:1240	arg1	delivery					1183:1190	effective transdermal delivery	1161:1190	effective transdermal delivery	1161:1190	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	4	30	theme	capillary	887:895	arg1	force					897:901	capillary force	887:901	capillary force	887:901	Fibroin scaffolds can instantly adsorb prepolymer solution due to capillary force, and subsequently initiate the formation of microneedles via photoinduced polymerization.					
30703318	3	31	theme	fibroin	623:629	arg1	scaffold					631:638	silk fibroin scaffold	618:638	silk fibroin scaffold	618:638	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	0	32	theme	Silk	14:17	arg1	Scaffold					27:34	Highly Porous Silk Fibroin Scaffold	0:34	Highly Porous Silk Fibroin Scaffold	0:34	Highly Porous Silk Fibroin Scaffold Packed in PEGDA/Sucrose Microneedles for Controllable Transdermal Drug Delivery.					
30703318	5	33	theme	model	1017:1021	arg1	drugs					1023:1027	model drugs	1017:1027	model drugs	1017:1027	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	1	34	theme	Polymeric	117:125	arg1	microneedles					127:138	Polymeric microneedles	117:138	Polymeric microneedles	117:138	Polymeric microneedles have attracted increasing attention as a minimally invasive platform for delivering drugs or vaccines in a more patient-friendly manner.					
30703318	2	35	theme	mass	485:488	arg1	production					490:499	the scaled-up mass production	471:499	the scaled-up mass production of polymeric microneedles	471:525	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	3	36	theme	-based	571:576	arg1	mold					587:590	a novel polydimethylsiloxane (PDMS)-based negative mold	536:590	a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold	536:638	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	5	37	theme	indocyanine	1059:1069	arg1	ICG					1078:1080	ICG	1078:1080	ICG	1078:1080	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	5	37	theme	indocyanine	1059:1069	arg1	green					1071:1075	indocyanine green	1059:1075	indocyanine green (ICG)	1059:1081	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	2	38	theme	scaled-up	475:483	arg1	production					490:499	the scaled-up mass production	471:499	the scaled-up mass production of polymeric microneedles	471:525	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	5	39	theme	sucrose	1260:1266	arg1	content					1268:1274	the sucrose content	1256:1274	the sucrose content	1256:1274	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	2	40	theme	polymeric	504:512	arg1	microneedles					514:525	polymeric microneedles	504:525	polymeric microneedles	504:525	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	0	41	theme	PEGDA/Sucrose	46:58	arg1	Microneedles					60:71	PEGDA/Sucrose Microneedles	46:71	PEGDA/Sucrose Microneedles for Controllable Transdermal Drug Delivery	46:114	Highly Porous Silk Fibroin Scaffold Packed in PEGDA/Sucrose Microneedles for Controllable Transdermal Drug Delivery.					
30703318	5	42	theme	controllable	1196:1207	arg1	release					1209:1215	controllable release	1196:1215	controllable release	1196:1215	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	4	43	theme	microneedles	947:958	arg1	formation					934:942	the formation	930:942	the formation of microneedles	930:958	Fibroin scaffolds can instantly adsorb prepolymer solution due to capillary force, and subsequently initiate the formation of microneedles via photoinduced polymerization.					
30703318	6	44	theme	presented	1281:1289	arg1	method					1291:1296	The presented method	1277:1296	The presented method	1277:1296	The presented method provides a simple strategy for quick fabrication of polymeric microneedles toward transdermal drug delivery applications.					
30703318	6	45	theme	delivery	1397:1404	arg1	applications					1406:1417	transdermal drug delivery applications	1380:1417	transdermal drug delivery applications	1380:1417	The presented method provides a simple strategy for quick fabrication of polymeric microneedles toward transdermal drug delivery applications.					
30703318	6	46	theme	transdermal	1380:1390	arg1	applications					1406:1417	transdermal drug delivery applications	1380:1417	transdermal drug delivery applications	1380:1417	The presented method provides a simple strategy for quick fabrication of polymeric microneedles toward transdermal drug delivery applications.					
30703318	3	47	theme	rapid	657:661	arg1	fabrication					663:673	rapid fabrication	657:673	rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix	657:818	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	2	48	theme	cumbersome	391:400	arg1	centrifugation					402:415	cumbersome centrifugation	391:415	cumbersome centrifugation	391:415	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	4	49	theme	prepolymer	860:869	arg1	solution					871:878	prepolymer solution	860:878	prepolymer solution due to capillary force	860:901	Fibroin scaffolds can instantly adsorb prepolymer solution due to capillary force, and subsequently initiate the formation of microneedles via photoinduced polymerization.					
30703318	3	50	theme	ethylene	751:758	arg1	poly					746:749	poly	746:749	poly(ethylene glycol) diacrylate (PEGDA)	746:785	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	3	50	theme	ethylene	751:758	arg1	glycol					760:765	ethylene glycol	751:765	ethylene glycol	751:765	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	2	51	theme	traditional	286:296	arg1	techniques					315:324	traditional microfabrication techniques	286:324	traditional microfabrication techniques using negative molds with needle-shaped cavities	286:373	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30703318	5	52	theme	drugs	1023:1027	arg1	doxorubicin					1088:1098	doxorubicin	1088:1098	doxorubicin (DOX)	1088:1104	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	5	52	theme	drugs	1023:1027	arg1	B					1050:1050	Rhodamine B	1040:1050	Rhodamine B (RhB)	1040:1056	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	5	52	theme	drugs	1023:1027	arg1	green					1071:1075	indocyanine green	1059:1075	indocyanine green (ICG)	1059:1081	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	5	52	theme	drugs	1023:1027	arg1	types					1008:1012	three types	1002:1012	three types	1002:1012	Based on three types of model drugs, including Rhodamine B (RhB), indocyanine green (ICG), and doxorubicin (DOX), the fabricated PEGDA/sucrose microneedles can realize effective transdermal delivery and controllable release of therapeutic molecules by regulating the sucrose content.					
30703318	6	53	theme	microneedles	1360:1371	arg1	fabrication					1335:1345	quick fabrication	1329:1345	quick fabrication of polymeric microneedles toward transdermal drug delivery applications	1329:1417	The presented method provides a simple strategy for quick fabrication of polymeric microneedles toward transdermal drug delivery applications.					
30703318	4	54	theme	Fibroin	821:827	arg1	scaffolds					829:837	Fibroin scaffolds	821:837	Fibroin scaffolds	821:837	Fibroin scaffolds can instantly adsorb prepolymer solution due to capillary force, and subsequently initiate the formation of microneedles via photoinduced polymerization.					
30703318	1	55	theme	increasing	155:164	arg1	platform					200:207	a minimally invasive platform	179:207	a minimally invasive platform for delivering drugs or vaccines in a more patient-friendly manner	179:274	Polymeric microneedles have attracted increasing attention as a minimally invasive platform for delivering drugs or vaccines in a more patient-friendly manner.					
30703318	1	55	theme	increasing	155:164	arg1	attention					166:174	increasing attention	155:174	increasing attention	155:174	Polymeric microneedles have attracted increasing attention as a minimally invasive platform for delivering drugs or vaccines in a more patient-friendly manner.					
30703318	3	56	theme	poly	746:749	arg1	diacrylate					768:777	poly(ethylene glycol) diacrylate	746:777	poly(ethylene glycol) diacrylate (PEGDA)	746:785	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	3	56	theme	poly	746:749	arg1	PEGDA					780:784	PEGDA	780:784	PEGDA	780:784	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	3	57	theme	polymeric	678:686	arg1	microneedles					688:699	polymeric microneedles	678:699	polymeric microneedles	678:699	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	1	58	theme	patient-friendly	252:267	arg1	manner					269:274	a more patient-friendly manner	245:274	a more patient-friendly manner	245:274	Polymeric microneedles have attracted increasing attention as a minimally invasive platform for delivering drugs or vaccines in a more patient-friendly manner.					
30703318	3	59	theme	diacrylate	768:777	arg1	composition					731:741	the composition	727:741	the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix	727:818	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	3	60	theme	microneedles	688:699	arg1	fabrication					663:673	rapid fabrication	657:673	rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix	657:818	Herein, a novel polydimethylsiloxane (PDMS)-based negative mold with cavities packed with silk fibroin scaffold is developed for rapid fabrication of polymeric microneedles, which comprise primarily the composition of poly(ethylene glycol) diacrylate (PEGDA) and sucrose as the needle matrix.					
30703318	2	61	theme	degassing	428:436	arg1	processes					438:446	vacuum degassing processes	421:446	vacuum degassing processes	421:446	However, traditional microfabrication techniques using negative molds with needle-shaped cavities usually require cumbersome centrifugation and vacuum degassing processes, which have restricted the scaled-up mass production of polymeric microneedles.					
30273946	9	0	from	complications	1616:1628	arg1	rats					1645:1648	alloxanized rats	1633:1648	alloxanized rats	1633:1648	The oil showed considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats.					
30273946	6	1	theme	oil-treated	895:905	arg1	rats					922:925	C. aurantifolia essential oil-treated hyperglycaemic rats	869:925	C. aurantifolia essential oil-treated hyperglycaemic rats	869:925	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	6	2	theme	decreased	1122:1130	arg1	predisposition					1132:1145	decreased predisposition	1122:1145	decreased predisposition of rats to cardiac risks	1122:1170	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	6	3	theme	density	994:1000	arg1	lipoprotein-cholesterol					1002:1024	low density lipoprotein-cholesterol	990:1024	low density lipoprotein-cholesterol	990:1024	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	5	4	theme	hepatic	730:736	arg1	glucose					738:744	hepatic glucose	730:744	hepatic glucose	730:744	to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased.					
30273946	1	5	theme	oil	171:173	arg1	composition					152:162	chemical composition	143:162	chemical composition of the oil	143:173	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	1	6	theme	alloxan-induced	279:293	arg1	rats					310:313	alloxan-induced hyperglycaemic rats	279:313	alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison	279:366	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	9	7	theme	dyslipidaemic	1602:1614	arg1	complications					1616:1628	hyperglycaemia-induced dyslipidaemic complications	1579:1628	hyperglycaemia-induced dyslipidaemic complications in alloxanized rats	1579:1648	The oil showed considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats.					
30273946	8	8	from	leaf	1406:1409	arg1	oil					1393:1395	essential oil	1383:1395	essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria	1383:1459	Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype.					
30273946	8	8	from	leaf	1406:1409	arg1	chemotype					1477:1485	a D-limonene chemotype	1464:1485	a D-limonene chemotype	1464:1485	Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype.					
30273946	1	9	theme	hyperglycaemic	295:308	arg1	rats					310:313	alloxan-induced hyperglycaemic rats	279:313	alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison	279:366	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	5	10	theme	significant	687:697	arg1	reduction					699:707	significant reduction	687:707	significant reduction in fasting blood and hepatic glucose	687:744	to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased.					
30273946	6	11	theme	C.	869:870	arg1	rats					922:925	C. aurantifolia essential oil-treated hyperglycaemic rats	869:925	C. aurantifolia essential oil-treated hyperglycaemic rats	869:925	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	8	12	theme	essential	1383:1391	arg1	oil					1393:1395	essential oil	1383:1395	essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria	1383:1459	Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype.					
30273946	8	12	theme	essential	1383:1391	arg1	chemotype					1477:1485	a D-limonene chemotype	1464:1485	a D-limonene chemotype	1464:1485	Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype.					
30273946	1	13	theme	Citrus	67:72	arg1	oil					102:104	Citrus aurantifolia leaf essential oil	67:104	Citrus aurantifolia leaf essential oil	67:104	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	6	14	theme	triacylglycerol	970:984	arg1	concentration					934:946	serum concentration	928:946	serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol	928:1024	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	9	15	theme	glucose	1516:1522	arg1	effect					1533:1538	considerable glucose lowering effect	1503:1538	considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats	1503:1648	The oil showed considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats.					
30273946	1	16	theme	aurantifolia	74:85	arg1	oil					102:104	Citrus aurantifolia leaf essential oil	67:104	Citrus aurantifolia leaf essential oil	67:104	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	3	17	theme	Other	457:461	arg1	compounds					471:479	Other notable compounds	457:479	Other notable compounds identified	457:490	Other notable compounds identified were neral (7.81%), linalool (4.75%), sulcatone (3.48%) and isogeraniol (3.48%).					
30273946	5	18	theme	hepatic	755:761	arg1	concentration					763:775	hepatic concentration	755:775	hepatic concentration of glycogen	755:787	to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased.					
30273946	1	19	theme	leaf	87:90	arg1	oil					102:104	Citrus aurantifolia leaf essential oil	67:104	Citrus aurantifolia leaf essential oil	67:104	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	3	20	theme	notable	463:469	arg1	compounds					471:479	Other notable compounds	457:479	Other notable compounds identified	457:490	Other notable compounds identified were neral (7.81%), linalool (4.75%), sulcatone (3.48%) and isogeraniol (3.48%).					
30273946	6	21	from	improvement	824:834	arg1	dyslipidaemia					839:851	dyslipidaemia	839:851	dyslipidaemia	839:851	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	9	22	theme	alloxanized	1633:1643	arg1	rats					1645:1648	alloxanized rats	1633:1648	alloxanized rats	1633:1648	The oil showed considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats.					
30273946	1	23	theme	essential	92:100	arg1	oil					102:104	Citrus aurantifolia leaf essential oil	67:104	Citrus aurantifolia leaf essential oil	67:104	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	7	24	theme	antidiabetic	1322:1333	arg1	drug					1335:1338	reference antidiabetic drug	1312:1338	reference antidiabetic drug	1312:1338	Antihyperglycaemic potential of administration of the oil was lower but compared favourably with the oral antihyperglycaemic agent used as reference antidiabetic drug.					
30273946	0	25	theme	Antidiabetic	0:11	arg1	Potentials					13:22	Antidiabetic Potentials	0:22	Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.	0:65	Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.					
30273946	8	26	from	study	1365:1369	arg1	data					1350:1353	data	1350:1353	data from this study	1350:1369	Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype.					
30273946	1	27	theme	gas	194:196	arg1	spectrometry					218:229	gas chromatography-mass spectrometry	194:229	gas chromatography-mass spectrometry	194:229	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	0	28	theme	Citrus	27:32	arg1	Oil					62:64	Citrus aurantifolia Leaf Essential Oil	27:64	Citrus aurantifolia Leaf Essential Oil	27:64	Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.					
30273946	6	29	theme	cholesterol	957:967	arg1	concentration					934:946	serum concentration	928:946	serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol	928:1024	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	6	30	located	observed	857:864	arg1	rats					922:925	C. aurantifolia essential oil-treated hyperglycaemic rats	869:925	C. aurantifolia essential oil-treated hyperglycaemic rats	869:925	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	6	30	located	observed	857:864	arg2	improvement					824:834	improvement	824:834	improvement in dyslipidaemia	824:851	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	8	31	theme	aurantifolia	1417:1428	arg1	leaf					1406:1409	the leaf	1402:1409	the leaf of C. aurantifolia grown in North-Central Nigeria	1402:1459	Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype.					
30273946	1	32	theme	chromatography-mass	198:216	arg1	spectrometry					218:229	gas chromatography-mass spectrometry	194:229	gas chromatography-mass spectrometry	194:229	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	2	33	theme	oil	452:454	arg1	constituent					433:443	the major constituent	423:443	the major constituent of the oil	423:454	Chemical analysis showed that D-limonene (57.84%) was the major constituent of the oil.					
30273946	2	33	theme	oil	452:454	arg1	D-limonene					399:408	D-limonene	399:408	D-limonene (57.84%)	399:417	Chemical analysis showed that D-limonene (57.84%) was the major constituent of the oil.					
30273946	6	34	theme	total	951:955	arg1	cholesterol					957:967	total cholesterol	951:967	total cholesterol	951:967	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	5	35	from	reduction	699:707	arg1	blood					720:724	fasting blood	712:724	fasting blood	712:724	to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased.					
30273946	5	35	from	reduction	699:707	arg1	glucose					738:744	hepatic glucose	730:744	hepatic glucose	730:744	to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased.					
30273946	7	36	theme	antihyperglycaemic	1279:1296	arg1	agent					1298:1302	the oral antihyperglycaemic agent	1270:1302	the oral antihyperglycaemic agent used as reference antidiabetic drug	1270:1338	Antihyperglycaemic potential of administration of the oil was lower but compared favourably with the oral antihyperglycaemic agent used as reference antidiabetic drug.					
30273946	9	37	theme	hyperglycaemia-induced	1579:1600	arg1	complications					1616:1628	hyperglycaemia-induced dyslipidaemic complications	1579:1628	hyperglycaemia-induced dyslipidaemic complications in alloxanized rats	1579:1648	The oil showed considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats.					
30273946	6	38	theme	density	1062:1068	arg1	lipoprotein-cholesterol					1070:1092	high density lipoprotein-cholesterol	1057:1092	high density lipoprotein-cholesterol	1057:1092	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	0	39	theme	Leaf	47:50	arg1	Oil					62:64	Citrus aurantifolia Leaf Essential Oil	27:64	Citrus aurantifolia Leaf Essential Oil	27:64	Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.					
30273946	6	40	theme	high	1057:1060	arg1	lipoprotein-cholesterol					1070:1092	high density lipoprotein-cholesterol	1057:1092	high density lipoprotein-cholesterol	1057:1092	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	4	41	theme	100 mg/Kg	628:636	arg1	b.wt					638:641	100 mg/Kg b.wt	628:641	100 mg/Kg b.wt.	628:642	Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.)					
30273946	4	42	theme	aurantifolia	610:621	arg1	oil					623:625	C. aurantifolia oil	607:625	C. aurantifolia oil	607:625	Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.)					
30273946	0	43	theme	aurantifolia	34:45	arg1	Oil					62:64	Citrus aurantifolia Leaf Essential Oil	27:64	Citrus aurantifolia Leaf Essential Oil	27:64	Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.					
30273946	1	44	theme	reference	338:346	arg1	drug					348:351	the reference drug	334:351	the reference drug for comparison	334:366	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	1	44	theme	reference	338:346	arg1	metformin					321:329	metformin	321:329	metformin	321:329	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	6	45	theme	low	990:992	arg1	lipoprotein-cholesterol					1002:1024	low density lipoprotein-cholesterol	990:1024	low density lipoprotein-cholesterol	990:1024	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	5	46	theme	glycogen	780:787	arg1	concentration					763:775	hepatic concentration	755:775	hepatic concentration of glycogen	755:787	to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased.					
30273946	2	47	theme	major	427:431	arg1	constituent					433:443	the major constituent	423:443	the major constituent of the oil	423:454	Chemical analysis showed that D-limonene (57.84%) was the major constituent of the oil.					
30273946	2	47	theme	major	427:431	arg1	D-limonene					399:408	D-limonene	399:408	D-limonene (57.84%)	399:417	Chemical analysis showed that D-limonene (57.84%) was the major constituent of the oil.					
30273946	0	48	theme	Oil	62:64	arg1	Potentials					13:22	Antidiabetic Potentials	0:22	Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.	0:65	Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.					
30273946	6	49	theme	serum	928:932	arg1	concentration					934:946	serum concentration	928:946	serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol	928:1024	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	9	50	theme	lowering	1524:1531	arg1	effect					1533:1538	considerable glucose lowering effect	1503:1538	considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats	1503:1648	The oil showed considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats.					
30273946	0	51	theme	Essential	52:60	arg1	Oil					62:64	Citrus aurantifolia Leaf Essential Oil	27:64	Citrus aurantifolia Leaf Essential Oil	27:64	Antidiabetic Potentials of Citrus aurantifolia Leaf Essential Oil.					
30273946	4	52	dep	administration	589:602	arg1	b.wt					638:641	100 mg/Kg b.wt	628:641	100 mg/Kg b.wt.	628:642	Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.)					
30273946	6	53	theme	cardiac	1158:1164	arg1	risks					1166:1170	cardiac risks	1158:1170	cardiac risks	1158:1170	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	7	54	theme	administration	1205:1218	arg1	potential					1192:1200	Antihyperglycaemic potential	1173:1200	Antihyperglycaemic potential of administration of the oil	1173:1229	Antihyperglycaemic potential of administration of the oil was lower but compared favourably with the oral antihyperglycaemic agent used as reference antidiabetic drug.					
30273946	5	55	dep	caused	680:685	arg1	whereas					747:753	whereas	747:753	whereas	747:753	to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased.					
30273946	6	56	dep	C.	869:870	arg1	essential					885:893	essential	885:893	essential	885:893	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	6	56	dep	C.	869:870	arg1	aurantifolia					872:883	aurantifolia	872:883	aurantifolia	872:883	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	7	57	theme	Antihyperglycaemic	1173:1190	arg1	potential					1192:1200	Antihyperglycaemic potential	1173:1200	Antihyperglycaemic potential of administration of the oil	1173:1229	Antihyperglycaemic potential of administration of the oil was lower but compared favourably with the oral antihyperglycaemic agent used as reference antidiabetic drug.					
30273946	2	58	theme	Chemical	369:376	arg1	analysis					378:385	Chemical analysis	369:385	Chemical analysis	369:385	Chemical analysis showed that D-limonene (57.84%) was the major constituent of the oil.					
30273946	6	59	theme	rats	1150:1153	arg1	predisposition					1132:1145	decreased predisposition	1122:1145	decreased predisposition of rats to cardiac risks	1122:1170	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	1	60	theme	antidiabetic	239:250	arg1	potentials					252:261	its antidiabetic potentials	235:261	its antidiabetic potentials	235:261	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	5	61	theme	hyperglycaemic	648:661	arg1	rats					663:666	hyperglycaemic rats	648:666	hyperglycaemic rats for 14 days	648:678	to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased.					
30273946	1	62	theme	chemical	143:150	arg1	composition					152:162	chemical composition	143:162	chemical composition of the oil	143:173	Citrus aurantifolia leaf essential oil was extracted via hydrodistillation, chemical composition of the oil was analyzed using gas chromatography-mass spectrometry and its antidiabetic potentials was assessed in alloxan-induced hyperglycaemic rats using metformin as the reference drug for comparison.					
30273946	7	63	theme	reference	1312:1320	arg1	drug					1335:1338	reference antidiabetic drug	1312:1338	reference antidiabetic drug	1312:1338	Antihyperglycaemic potential of administration of the oil was lower but compared favourably with the oral antihyperglycaemic agent used as reference antidiabetic drug.					
30273946	5	64	theme	fasting	712:718	arg1	blood					720:724	fasting blood	712:724	fasting blood	712:724	to hyperglycaemic rats for 14 days caused significant reduction in fasting blood and hepatic glucose, whereas hepatic concentration of glycogen was significantly increased.					
30273946	4	65	theme	C.	607:608	arg1	oil					623:625	C. aurantifolia oil	607:625	C. aurantifolia oil	607:625	Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.)					
30273946	9	66	theme	considerable	1503:1514	arg1	effect					1533:1538	considerable glucose lowering effect	1503:1538	considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats	1503:1648	The oil showed considerable glucose lowering effect as well as the potential to ameliorate hyperglycaemia-induced dyslipidaemic complications in alloxanized rats.					
30273946	8	67	theme	North-Central	1439:1451	arg1	Nigeria					1453:1459	North-Central Nigeria	1439:1459	North-Central Nigeria	1439:1459	Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype.					
30273946	4	68	theme	oil	623:625	arg1	administration					589:602	Intraperitoneal administration	573:602	Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.)	573:643	Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.)					
30273946	4	69	theme	Intraperitoneal	573:587	arg1	administration					589:602	Intraperitoneal administration	573:602	Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.)	573:643	Intraperitoneal administration of C. aurantifolia oil (100 mg/Kg b.wt.)					
30273946	7	70	theme	oral	1274:1277	arg1	agent					1298:1302	the oral antihyperglycaemic agent	1270:1302	the oral antihyperglycaemic agent used as reference antidiabetic drug	1270:1338	Antihyperglycaemic potential of administration of the oil was lower but compared favourably with the oral antihyperglycaemic agent used as reference antidiabetic drug.					
30273946	6	71	theme	hyperglycaemic	907:920	arg1	rats					922:925	C. aurantifolia essential oil-treated hyperglycaemic rats	869:925	C. aurantifolia essential oil-treated hyperglycaemic rats	869:925	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
30273946	8	72	theme	D-limonene	1466:1475	arg1	oil					1393:1395	essential oil	1383:1395	essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria	1383:1459	Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype.					
30273946	8	72	theme	D-limonene	1466:1475	arg1	chemotype					1477:1485	a D-limonene chemotype	1464:1485	a D-limonene chemotype	1464:1485	Overall, data from this study showed that essential oil from the leaf of C. aurantifolia grown in North-Central Nigeria is a D-limonene chemotype.					
30273946	7	73	theme	oil	1227:1229	arg1	administration					1205:1218	administration	1205:1218	administration of the oil	1205:1229	Antihyperglycaemic potential of administration of the oil was lower but compared favourably with the oral antihyperglycaemic agent used as reference antidiabetic drug.					
30273946	6	74	theme	lipoprotein-cholesterol	1002:1024	arg1	concentration					934:946	serum concentration	928:946	serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol	928:1024	Also, improvement in dyslipidaemia was observed in C. aurantifolia essential oil-treated hyperglycaemic rats; serum concentration of total cholesterol, triacylglycerol and low density lipoprotein-cholesterol were significantly reduced and high density lipoprotein-cholesterol was increased, resulting in decreased predisposition of rats to cardiac risks.					
31618426	9	0	theme	germ-free	896:904	arg1	mice					914:917	germ-free (GF) B6 mice	896:917	germ-free (GF) B6 mice	896:917	A separate group of germ-free (GF) B6 mice was also topically challenged with LPS.					
31618426	2	1	theme	oral	342:345	arg1	antibiotics					347:357	oral antibiotics	342:357	oral antibiotics	342:357	Methods Dysbiosis was induced by oral antibiotics for 14 days in a group of conventional female C57BL/6J (B6) mice.					
31618426	1	2	theme	antibiotic	252:261	arg1	dysbiosis					298:306	antibiotic (antibiotic cocktail, ABX) induced dysbiosis	252:306	antibiotic (antibiotic cocktail, ABX) induced dysbiosis	252:306	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	9	3	theme	GF	907:908	arg1	mice					914:917	germ-free (GF) B6 mice	896:917	germ-free (GF) B6 mice	896:917	A separate group of germ-free (GF) B6 mice was also topically challenged with LPS.					
31618426	1	4	theme	antibiotic	264:273	arg1	ABX					285:287	ABX	285:287	ABX	285:287	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	1	4	theme	antibiotic	264:273	arg1	cocktail					275:282	antibiotic cocktail	264:282	antibiotic cocktail	264:282	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	9	5	theme	B6	911:912	arg1	mice					914:917	germ-free (GF) B6 mice	896:917	germ-free (GF) B6 mice	896:917	A separate group of germ-free (GF) B6 mice was also topically challenged with LPS.					
31618426	14	6	dep	greater	1598:1604	arg1	IL-12					1606:1610	IL-12	1606:1610	IL-12	1606:1610	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	1	7	theme	applied	194:200	arg1	LPS					222:224	LPS	222:224	LPS	222:224	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	1	7	theme	applied	194:200	arg1	lipopolysaccharide					202:219	lipopolysaccharide	202:219	topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis	184:306	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	14	8	theme	GF	1649:1650	arg1	conjunctiva					1652:1662	GF conjunctiva	1649:1662	GF conjunctiva	1649:1662	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	13	9	theme	higher	1387:1392	arg1	expression					1419:1428	higher TNF-α, CXCL10, and IL-12 expression	1387:1428	higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice	1387:1466	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	7	10	theme	LPS	709:711	arg1	dose					701:704	a single dose	692:704	a single dose of LPS or vehicle	692:722	At day 15, a single dose of LPS or vehicle was topically applied to ABX and naïve mice.					
31618426	10	11	theme	Antibiotic	967:976	arg1	treatment					978:986	Results Antibiotic treatment	959:986	Results Antibiotic treatment	959:986	Results Antibiotic treatment significantly decreased intestinal diversity and increased serum levels of LPS.					
31618426	13	12	theme	intact	1502:1507	arg1	microbiota					1509:1518	intact microbiota	1502:1518	intact microbiota	1502:1518	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	1	13	from	lipopolysaccharide	202:219	arg1	mice					230:233	mice	230:233	mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis	230:306	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	6	14	from	expression	620:629	arg1	nodes					643:647	draining nodes	634:647	draining nodes	634:647	CD86 expression in draining nodes was examined by flow cytometry.					
31618426	15	15	theme	Conclusions	1707:1717	arg1	depletion					1725:1733	Conclusions Acute depletion	1707:1733	Conclusions Acute depletion of commensals through antibiotics or germ-free environment	1707:1792	Conclusions Acute depletion of commensals through antibiotics or germ-free environment worsens the inflammatory response to LPS.					
31618426	10	16	theme	intestinal	1012:1021	arg1	diversity					1023:1031	intestinal diversity	1012:1031	intestinal diversity	1012:1031	Results Antibiotic treatment significantly decreased intestinal diversity and increased serum levels of LPS.					
31618426	13	17	theme	mice	1463:1466	arg1	cornea					1437:1442	the cornea	1433:1442	the cornea of LPS-treated ABX mice	1433:1466	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	1	18	theme	inflammatory	133:144	arg1	response					146:153	the inflammatory response	129:153	the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis	129:306	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	14	19	theme	LPS	1521:1523	arg1	stimulation					1525:1535	LPS stimulation	1521:1535	LPS stimulation on GF conjunctiva	1521:1553	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	14	20	theme	greater	1598:1604	arg1	expression					1622:1631	greater IL-12 and IFN-γ expression	1598:1631	greater IL-12 and IFN-γ expression	1598:1631	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	14	21	located	observed	1637:1644	arg1	conjunctiva					1652:1662	GF conjunctiva	1649:1662	GF conjunctiva	1649:1662	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	14	21	located	observed	1637:1644	arg2	expression					1622:1631	greater IL-12 and IFN-γ expression	1598:1631	greater IL-12 and IFN-γ expression	1598:1631	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	15	22	theme	inflammatory	1806:1817	arg1	response					1819:1826	the inflammatory response	1802:1826	the inflammatory response to LPS	1802:1833	Conclusions Acute depletion of commensals through antibiotics or germ-free environment worsens the inflammatory response to LPS.					
31618426	4	23	theme	Intestinal	482:491	arg1	differences					504:514	Intestinal microbiome differences	482:514	Intestinal microbiome differences	482:514	Intestinal microbiome differences were assessed using 16S rRNA sequencing of fecal pellet DNA.					
31618426	10	24	theme	serum	1047:1051	arg1	levels					1053:1058	increased serum levels	1037:1058	increased serum levels of LPS	1037:1065	Results Antibiotic treatment significantly decreased intestinal diversity and increased serum levels of LPS.					
31618426	6	25	theme	CD86	615:618	arg1	expression					620:629	CD86 expression	615:629	CD86 expression in draining nodes	615:647	CD86 expression in draining nodes was examined by flow cytometry.					
31618426	8	26	theme	Corneal	769:775	arg1	epithelium					777:786	Corneal epithelium	769:786	Corneal epithelium	769:786	Corneal epithelium and conjunctiva were obtained after 4 hours and processed for gene expression analysis.					
31618426	14	27	theme	ABX	1579:1581	arg1	mice					1583:1586	ABX mice	1579:1586	ABX mice	1579:1586	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	2	28	theme	female	398:403	arg1	mice					419:422	conventional female C57BL/6J (B6) mice	385:422	conventional female C57BL/6J (B6) mice	385:422	Methods Dysbiosis was induced by oral antibiotics for 14 days in a group of conventional female C57BL/6J (B6) mice.					
31618426	15	29	theme	germ-free	1772:1780	arg1	environment					1782:1792	germ-free environment	1772:1792	germ-free environment	1772:1792	Conclusions Acute depletion of commensals through antibiotics or germ-free environment worsens the inflammatory response to LPS.					
31618426	13	30	with	mice	1492:1495	arg1	microbiota					1509:1518	intact microbiota	1502:1518	intact microbiota	1502:1518	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	8	31	theme	gene	850:853	arg1	analysis					866:873	gene expression analysis	850:873	gene expression analysis	850:873	Corneal epithelium and conjunctiva were obtained after 4 hours and processed for gene expression analysis.					
31618426	2	32	theme	mice	419:422	arg1	mice					419:422	conventional female C57BL/6J (B6) mice	385:422	conventional female C57BL/6J (B6) mice	385:422	Methods Dysbiosis was induced by oral antibiotics for 14 days in a group of conventional female C57BL/6J (B6) mice.					
31618426	2	32	theme	mice	419:422	arg1	group					376:380	a group	374:380	a group of conventional female C57BL/6J (B6) mice	374:422	Methods Dysbiosis was induced by oral antibiotics for 14 days in a group of conventional female C57BL/6J (B6) mice.					
31618426	1	33	dep	dysbiosis	298:306	arg1	ABX					285:287	ABX	285:287	ABX	285:287	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	1	33	dep	dysbiosis	298:306	arg1	cocktail					275:282	antibiotic cocktail	264:282	antibiotic cocktail	264:282	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	2	34	theme	B6	415:416	arg1	mice					419:422	conventional female C57BL/6J (B6) mice	385:422	conventional female C57BL/6J (B6) mice	385:422	Methods Dysbiosis was induced by oral antibiotics for 14 days in a group of conventional female C57BL/6J (B6) mice.					
31618426	15	35	theme	commensals	1738:1747	arg1	depletion					1725:1733	Conclusions Acute depletion	1707:1733	Conclusions Acute depletion of commensals through antibiotics or germ-free environment	1707:1792	Conclusions Acute depletion of commensals through antibiotics or germ-free environment worsens the inflammatory response to LPS.					
31618426	3	36	theme	rRNA	429:432	arg1	sequencing					434:443	16S rRNA sequencing	425:443	16S rRNA sequencing	425:443	16S rRNA sequencing investigated microbiome composition.					
31618426	0	37	theme	Ocular	20:25	arg1	Response					48:55	Ocular Surface Inflammatory Response	20:55	Ocular Surface Inflammatory Response to Liposaccharide	20:73	Dysbiosis Modulates Ocular Surface Inflammatory Response to Liposaccharide.					
31618426	7	38	theme	naïve	757:761	arg1	mice					763:766	ABX and naïve mice	749:766	ABX and naïve mice	749:766	At day 15, a single dose of LPS or vehicle was topically applied to ABX and naïve mice.					
31618426	4	39	theme	fecal	559:563	arg1	DNA					572:574	fecal pellet DNA	559:574	fecal pellet DNA	559:574	Intestinal microbiome differences were assessed using 16S rRNA sequencing of fecal pellet DNA.					
31618426	6	40	theme	flow	665:668	arg1	cytometry					670:678	flow cytometry	665:678	flow cytometry	665:678	CD86 expression in draining nodes was examined by flow cytometry.					
31618426	2	41	theme	conventional	385:396	arg1	mice					419:422	conventional female C57BL/6J (B6) mice	385:422	conventional female C57BL/6J (B6) mice	385:422	Methods Dysbiosis was induced by oral antibiotics for 14 days in a group of conventional female C57BL/6J (B6) mice.					
31618426	0	42	theme	Inflammatory	35:46	arg1	Response					48:55	Ocular Surface Inflammatory Response	20:55	Ocular Surface Inflammatory Response to Liposaccharide	20:73	Dysbiosis Modulates Ocular Surface Inflammatory Response to Liposaccharide.					
31618426	1	43	theme	induced	290:296	arg1	dysbiosis					298:306	antibiotic (antibiotic cocktail, ABX) induced dysbiosis	252:306	antibiotic (antibiotic cocktail, ABX) induced dysbiosis	252:306	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	11	44	theme	significant	1094:1104	arg1	increase					1106:1113	a significant increase	1092:1113	a significant increase in CD86+MHC II+CD11c+CD11b+ cells in draining nodes	1092:1165	This was accompanied by a significant increase in CD86+MHC II+CD11c+CD11b+ cells in draining nodes.					
31618426	12	45	theme	CXCL10	1239:1244	arg1	transcripts					1251:1261	CXCL10 mRNA transcripts	1239:1261	CXCL10 mRNA transcripts	1239:1261	Compared to vehicle, topically applied LPS increased IL-1β, TNF-α, and CXCL10 mRNA transcripts in cornea and IL-1β, TNF-α, and CXCL10 in the conjunctiva in conventional and antibiotic-treated groups.					
31618426	7	46	theme	ABX	749:751	arg1	mice					763:766	ABX and naïve mice	749:766	ABX and naïve mice	749:766	At day 15, a single dose of LPS or vehicle was topically applied to ABX and naïve mice.					
31618426	13	47	theme	TNF-α	1394:1398	arg1	expression					1419:1428	higher TNF-α, CXCL10, and IL-12 expression	1387:1428	higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice	1387:1466	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	4	48	theme	16S	536:538	arg1	sequencing					545:554	16S rRNA sequencing	536:554	16S rRNA sequencing of fecal pellet DNA	536:574	Intestinal microbiome differences were assessed using 16S rRNA sequencing of fecal pellet DNA.					
31618426	13	49	theme	CXCL10	1401:1406	arg1	expression					1419:1428	higher TNF-α, CXCL10, and IL-12 expression	1387:1428	higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice	1387:1466	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	11	50	theme	II+CD11c+CD11b+	1127:1141	arg1	cells					1143:1147	CD86+MHC II+CD11c+CD11b+ cells	1118:1147	CD86+MHC II+CD11c+CD11b+ cells in draining nodes	1118:1165	This was accompanied by a significant increase in CD86+MHC II+CD11c+CD11b+ cells in draining nodes.					
31618426	9	51	theme	separate	878:885	arg1	mice					914:917	germ-free (GF) B6 mice	896:917	germ-free (GF) B6 mice	896:917	A separate group of germ-free (GF) B6 mice was also topically challenged with LPS.					
31618426	9	51	theme	separate	878:885	arg1	group					887:891	A separate group	876:891	A separate group of germ-free (GF) B6 mice	876:917	A separate group of germ-free (GF) B6 mice was also topically challenged with LPS.					
31618426	14	52	from	stimulation	1525:1535	arg1	conjunctiva					1543:1553	GF conjunctiva	1540:1553	GF conjunctiva	1540:1553	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	6	53	theme	draining	634:641	arg1	nodes					643:647	draining nodes	634:647	draining nodes	634:647	CD86 expression in draining nodes was examined by flow cytometry.					
31618426	14	54	theme	conventional	1676:1687	arg1	mice					1701:1704	conventional LPS-treated mice	1676:1704	conventional LPS-treated mice	1676:1704	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	12	55	theme	applied	1199:1205	arg1	LPS					1207:1209	topically applied LPS	1189:1209	topically applied LPS	1189:1209	Compared to vehicle, topically applied LPS increased IL-1β, TNF-α, and CXCL10 mRNA transcripts in cornea and IL-1β, TNF-α, and CXCL10 in the conjunctiva in conventional and antibiotic-treated groups.					
31618426	4	56	theme	DNA	572:574	arg1	sequencing					545:554	16S rRNA sequencing	536:554	16S rRNA sequencing of fecal pellet DNA	536:574	Intestinal microbiome differences were assessed using 16S rRNA sequencing of fecal pellet DNA.					
31618426	7	57	theme	single	694:699	arg1	dose					701:704	a single dose	692:704	a single dose of LPS or vehicle	692:722	At day 15, a single dose of LPS or vehicle was topically applied to ABX and naïve mice.					
31618426	12	58	theme	antibiotic-treated	1341:1358	arg1	groups					1360:1365	conventional and antibiotic-treated groups	1324:1365	conventional and antibiotic-treated groups	1324:1365	Compared to vehicle, topically applied LPS increased IL-1β, TNF-α, and CXCL10 mRNA transcripts in cornea and IL-1β, TNF-α, and CXCL10 in the conjunctiva in conventional and antibiotic-treated groups.					
31618426	14	59	from	results	1568:1574	arg1	mice					1583:1586	ABX mice	1579:1586	ABX mice	1579:1586	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	1	60	theme	conjunctiva	169:179	arg1	response					146:153	the inflammatory response	129:153	the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis	129:306	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	9	61	theme	mice	914:917	arg1	mice					914:917	germ-free (GF) B6 mice	896:917	germ-free (GF) B6 mice	896:917	A separate group of germ-free (GF) B6 mice was also topically challenged with LPS.					
31618426	9	61	theme	mice	914:917	arg1	group					887:891	A separate group	876:891	A separate group of germ-free (GF) B6 mice	876:917	A separate group of germ-free (GF) B6 mice was also topically challenged with LPS.					
31618426	12	62	theme	conventional	1324:1335	arg1	groups					1360:1365	conventional and antibiotic-treated groups	1324:1365	conventional and antibiotic-treated groups	1324:1365	Compared to vehicle, topically applied LPS increased IL-1β, TNF-α, and CXCL10 mRNA transcripts in cornea and IL-1β, TNF-α, and CXCL10 in the conjunctiva in conventional and antibiotic-treated groups.					
31618426	13	63	theme	LPS-treated	1480:1490	arg1	mice					1492:1495	LPS-treated mice	1480:1495	LPS-treated mice with intact microbiota	1480:1518	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	13	64	from	expression	1419:1428	arg1	cornea					1437:1442	the cornea	1433:1442	the cornea of LPS-treated ABX mice	1433:1466	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	10	65	theme	Results	959:965	arg1	treatment					978:986	Results Antibiotic treatment	959:986	Results Antibiotic treatment	959:986	Results Antibiotic treatment significantly decreased intestinal diversity and increased serum levels of LPS.					
31618426	14	66	theme	GF	1540:1541	arg1	conjunctiva					1543:1553	GF conjunctiva	1540:1553	GF conjunctiva	1540:1553	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	14	67	theme	IFN-γ	1616:1620	arg1	expression					1622:1631	greater IL-12 and IFN-γ expression	1598:1631	greater IL-12 and IFN-γ expression	1598:1631	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
31618426	13	68	theme	ABX	1459:1461	arg1	mice					1463:1466	LPS-treated ABX mice	1447:1466	LPS-treated ABX mice	1447:1466	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	1	69	dep	Purpose	76:82	arg1	was					110:112	was	110:112	was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis	110:306	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	4	70	theme	microbiome	493:502	arg1	differences					504:514	Intestinal microbiome differences	482:514	Intestinal microbiome differences	482:514	Intestinal microbiome differences were assessed using 16S rRNA sequencing of fecal pellet DNA.					
31618426	11	71	from	cells	1143:1147	arg1	nodes					1161:1165	draining nodes	1152:1165	draining nodes	1152:1165	This was accompanied by a significant increase in CD86+MHC II+CD11c+CD11b+ cells in draining nodes.					
31618426	1	72	theme	cornea	158:163	arg1	response					146:153	the inflammatory response	129:153	the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis	129:306	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	10	73	theme	increased	1037:1045	arg1	levels					1053:1058	increased serum levels	1037:1058	increased serum levels of LPS	1037:1065	Results Antibiotic treatment significantly decreased intestinal diversity and increased serum levels of LPS.					
31618426	11	74	theme	draining	1152:1159	arg1	nodes					1161:1165	draining nodes	1152:1165	draining nodes	1152:1165	This was accompanied by a significant increase in CD86+MHC II+CD11c+CD11b+ cells in draining nodes.					
31618426	11	75	from	increase	1106:1113	arg1	cells					1143:1147	CD86+MHC II+CD11c+CD11b+ cells	1118:1147	CD86+MHC II+CD11c+CD11b+ cells in draining nodes	1118:1165	This was accompanied by a significant increase in CD86+MHC II+CD11c+CD11b+ cells in draining nodes.					
31618426	2	76	theme	C57BL/6J	405:412	arg1	mice					419:422	conventional female C57BL/6J (B6) mice	385:422	conventional female C57BL/6J (B6) mice	385:422	Methods Dysbiosis was induced by oral antibiotics for 14 days in a group of conventional female C57BL/6J (B6) mice.					
31618426	10	77	theme	LPS	1063:1065	arg1	levels					1053:1058	increased serum levels	1037:1058	increased serum levels of LPS	1037:1065	Results Antibiotic treatment significantly decreased intestinal diversity and increased serum levels of LPS.					
31618426	10	77	theme	LPS	1063:1065	arg1	diversity					1023:1031	intestinal diversity	1012:1031	intestinal diversity	1012:1031	Results Antibiotic treatment significantly decreased intestinal diversity and increased serum levels of LPS.					
31618426	13	78	theme	LPS-treated	1447:1457	arg1	mice					1463:1466	LPS-treated ABX mice	1447:1466	LPS-treated ABX mice	1447:1466	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	0	79	theme	Surface	27:33	arg1	Response					48:55	Ocular Surface Inflammatory Response	20:55	Ocular Surface Inflammatory Response to Liposaccharide	20:73	Dysbiosis Modulates Ocular Surface Inflammatory Response to Liposaccharide.					
31618426	1	80	theme	study	104:108	arg1	purpose					88:94	The purpose	84:94	The purpose of this study	84:108	Purpose The purpose of this study was to investigate the inflammatory response of cornea and conjunctiva to topically applied lipopolysaccharide (LPS) in mice with and without antibiotic (antibiotic cocktail, ABX) induced dysbiosis.					
31618426	8	81	theme	expression	855:864	arg1	analysis					866:873	gene expression analysis	850:873	gene expression analysis	850:873	Corneal epithelium and conjunctiva were obtained after 4 hours and processed for gene expression analysis.					
31618426	4	82	theme	pellet	565:570	arg1	DNA					572:574	fecal pellet DNA	559:574	fecal pellet DNA	559:574	Intestinal microbiome differences were assessed using 16S rRNA sequencing of fecal pellet DNA.					
31618426	15	83	theme	Acute	1719:1723	arg1	depletion					1725:1733	Conclusions Acute depletion	1707:1733	Conclusions Acute depletion of commensals through antibiotics or germ-free environment	1707:1792	Conclusions Acute depletion of commensals through antibiotics or germ-free environment worsens the inflammatory response to LPS.					
31618426	13	84	theme	IL-12	1413:1417	arg1	expression					1419:1428	higher TNF-α, CXCL10, and IL-12 expression	1387:1428	higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice	1387:1466	However, there was higher TNF-α, CXCL10, and IL-12 expression in the cornea of LPS-treated ABX mice compared to LPS-treated mice with intact microbiota.					
31618426	12	85	from	conjunctiva	1309:1319	arg1	groups					1360:1365	conventional and antibiotic-treated groups	1324:1365	conventional and antibiotic-treated groups	1324:1365	Compared to vehicle, topically applied LPS increased IL-1β, TNF-α, and CXCL10 mRNA transcripts in cornea and IL-1β, TNF-α, and CXCL10 in the conjunctiva in conventional and antibiotic-treated groups.					
31618426	12	86	theme	mRNA	1246:1249	arg1	transcripts					1251:1261	CXCL10 mRNA transcripts	1239:1261	CXCL10 mRNA transcripts	1239:1261	Compared to vehicle, topically applied LPS increased IL-1β, TNF-α, and CXCL10 mRNA transcripts in cornea and IL-1β, TNF-α, and CXCL10 in the conjunctiva in conventional and antibiotic-treated groups.					
31618426	3	87	theme	16S	425:427	arg1	sequencing					434:443	16S rRNA sequencing	425:443	16S rRNA sequencing	425:443	16S rRNA sequencing investigated microbiome composition.					
31618426	4	88	theme	rRNA	540:543	arg1	sequencing					545:554	16S rRNA sequencing	536:554	16S rRNA sequencing of fecal pellet DNA	536:574	Intestinal microbiome differences were assessed using 16S rRNA sequencing of fecal pellet DNA.					
31618426	11	89	theme	CD86+MHC	1118:1125	arg1	cells					1143:1147	CD86+MHC II+CD11c+CD11b+ cells	1118:1147	CD86+MHC II+CD11c+CD11b+ cells in draining nodes	1118:1165	This was accompanied by a significant increase in CD86+MHC II+CD11c+CD11b+ cells in draining nodes.					
31618426	3	90	theme	microbiome	458:467	arg1	composition					469:479	microbiome composition	458:479	microbiome composition	458:479	16S rRNA sequencing investigated microbiome composition.					
31618426	7	91	theme	vehicle	716:722	arg1	dose					701:704	a single dose	692:704	a single dose of LPS or vehicle	692:722	At day 15, a single dose of LPS or vehicle was topically applied to ABX and naïve mice.					
31618426	14	92	theme	LPS-treated	1689:1699	arg1	mice					1701:1704	conventional LPS-treated mice	1676:1704	conventional LPS-treated mice	1676:1704	LPS stimulation on GF conjunctiva mirrored the results in ABX mice, although greater IL-12 and IFN-γ expression was observed in GF conjunctiva compared to conventional LPS-treated mice.					
29622193	0	0	theme	selective	83:91	arg1	extraction					105:114	selective solid phase extraction	83:114	selective solid phase extraction of lead ions in food and water samples	83:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	2	1	theme	3D	445:446	arg1	framework					448:456	porous 3D framework	438:456	porous 3D framework of hydrogel	438:468	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	2	2	theme	monolithic	545:554	arg1	column					556:561	the hydrogel-based hybrid monolithic column	519:561	the hydrogel-based hybrid monolithic column	519:561	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	4	3	theme	method	841:846	arg1	conditions					827:836	the optimal experiment conditions	804:836	the optimal experiment conditions of method	804:846	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	4	4	theme	2.65	957:960	arg1	limit					853:857	the limit	849:857	the limit of detection, preconcentration factor and precision as RSD%	849:917	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	4	4	theme	2.65	957:960	arg1	%					961:961	0.39 μg L-1, 53.7 and 2.65%	935:961	%	961:961	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	1	5	theme	solid	230:234	arg1	extraction					242:251	solid phase extraction	230:251	solid phase extraction of Pb(II) in food and water samples	230:287	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	2	6	theme	porous	438:443	arg1	framework					448:456	porous 3D framework	438:456	porous 3D framework of hydrogel	438:468	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	3	7	theme	analytes	765:772	arg1	recoveries					747:756	the recoveries	743:756	the recoveries of the analytes	743:772	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	2	8	theme	hybrid	538:543	arg1	column					556:561	the hydrogel-based hybrid monolithic column	519:561	the hydrogel-based hybrid monolithic column	519:561	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	0	9	from	samples	147:153	arg1	extraction					105:114	selective solid phase extraction	83:114	selective solid phase extraction of lead ions in food and water samples	83:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	0	10	theme	phase	99:103	arg1	extraction					105:114	selective solid phase extraction	83:114	selective solid phase extraction of lead ions in food and water samples	83:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	1	11	theme	hybrid	292:297	arg1	column					310:315	a hybrid monolithic column	290:315	a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel	290:386	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	2	12	theme	hydrogel-based	523:536	arg1	column					556:561	the hydrogel-based hybrid monolithic column	519:561	the hydrogel-based hybrid monolithic column	519:561	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	0	13	theme	solid	93:97	arg1	extraction					105:114	selective solid phase extraction	83:114	selective solid phase extraction of lead ions in food and water samples	83:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	1	14	theme	monolithic	299:308	arg1	column					310:315	a hybrid monolithic column	290:315	a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel	290:386	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	4	15	theme	optimal	808:814	arg1	conditions					827:836	the optimal experiment conditions	804:836	the optimal experiment conditions of method	804:846	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	2	16	theme	enrichment	578:587	arg1	selectivity					589:599	enhanced enrichment selectivity	569:599	enhanced enrichment selectivity	569:599	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	6	17	dep	water	1135:1139	arg1	samples					1154:1160	samples	1154:1160	samples	1154:1160	Finally, the method was successfully verified by analyzing spiked Pb2+ in water and drinking samples.					
29622193	2	18	theme	hydrogel	461:468	arg1	framework					448:456	porous 3D framework	438:456	porous 3D framework of hydrogel	438:468	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	3	19	theme	pH	666:667	arg1	Effects					633:639	Effects	633:639	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes	633:772	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	2	20	theme	enhanced	569:576	arg1	selectivity					589:599	enhanced enrichment selectivity	569:599	enhanced enrichment selectivity	569:599	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	4	21	theme	0.39 μg L-1	935:945	arg1	limit					853:857	the limit	849:857	the limit of detection, preconcentration factor and precision as RSD%	849:917	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	4	21	theme	0.39 μg L-1	935:945	arg1	%					961:961	0.39 μg L-1, 53.7 and 2.65%	935:961	%	961:961	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	4	22	theme	detection	862:870	arg1	limit					853:857	the limit	849:857	the limit of detection, preconcentration factor and precision as RSD%	849:917	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	4	22	theme	detection	862:870	arg1	%					961:961	0.39 μg L-1, 53.7 and 2.65%	935:961	%	961:961	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	5	23	theme	recoveries	1044:1053	arg1	%					1058:1058	recoveries >97%	1044:1058	recoveries >97%	1044:1058	Trace Pb(II) can be quantitatively preconcentrated at pH 6.0 with recoveries >97%.					
29622193	0	24	theme	hybrid	2:7	arg1	column					20:25	A hybrid monolithic column	0:25	A hybrid monolithic column	0:25	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	3	25	from	Effects	633:639	arg1	recoveries					747:756	the recoveries	743:756	the recoveries of the analytes	743:772	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	6	26	from	Pb2+	1127:1130	arg1	water					1135:1139	water	1135:1139	water	1135:1139	Finally, the method was successfully verified by analyzing spiked Pb2+ in water and drinking samples.					
29622193	6	26	from	Pb2+	1127:1130	arg1	drinking					1145:1152	drinking	1145:1152	drinking	1145:1152	Finally, the method was successfully verified by analyzing spiked Pb2+ in water and drinking samples.					
29622193	3	27	theme	eluent	696:701	arg1	concentration					670:682	concentration	670:682	concentration	670:682	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	3	27	theme	eluent	696:701	arg1	volume					711:716	sample volume	704:716	sample volume	704:716	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	3	27	theme	eluent	696:701	arg1	composition					653:663	hydrogel composition	644:663	hydrogel composition	644:663	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	3	27	theme	eluent	696:701	arg1	type					688:691	type	688:691	type	688:691	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	3	27	theme	eluent	696:701	arg1	ions					735:738	interfering ions	723:738	interfering ions	723:738	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	3	27	theme	eluent	696:701	arg1	pH					666:667	pH	666:667	pH	666:667	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	4	28	theme	preconcentration	873:888	arg1	factor					890:895	preconcentration factor	873:895	preconcentration factor	873:895	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	4	28	theme	preconcentration	873:888	arg1	detection					862:870	detection	862:870	detection	862:870	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	3	29	theme	hydrogel	644:651	arg1	composition					653:663	hydrogel composition	644:663	hydrogel composition	644:663	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	2	30	theme	LDHs	499:502	arg1	adsorption					485:494	selective adsorption	475:494	selective adsorption of LDHs toward Pb(II)	475:516	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	1	31	theme	Pb	256:257	arg1	extraction					242:251	solid phase extraction	230:251	solid phase extraction of Pb(II) in food and water samples	230:287	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	5	32	theme	Trace	978:982	arg1	II					987:988	II	987:988	II	987:988	Trace Pb(II) can be quantitatively preconcentrated at pH 6.0 with recoveries >97%.					
29622193	5	32	theme	Trace	978:982	arg1	Pb					984:985	Trace Pb	978:985	Trace Pb(II)	978:989	Trace Pb(II) can be quantitatively preconcentrated at pH 6.0 with recoveries >97%.					
29622193	0	33	theme	ions	124:127	arg1	extraction					105:114	selective solid phase extraction	83:114	selective solid phase extraction of lead ions in food and water samples	83:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	2	34	theme	target	620:625	arg1	ions					627:630	target ions	620:630	target ions	620:630	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	0	35	theme	monolithic	9:18	arg1	column					20:25	A hybrid monolithic column	0:25	A hybrid monolithic column	0:25	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	0	36	theme	lead	119:122	arg1	ions					124:127	lead ions	119:127	lead ions in food and water samples	119:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	1	37	theme	layered	326:332	arg1	hydrogel					379:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	1	38	theme	accurate	179:186	arg1	method					214:219	an accurate and precise determination method	176:219	an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples	176:287	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	0	39	theme	food	132:135	arg1	samples					147:153	food and water samples	132:153	food and water samples	132:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	1	40	theme	double	334:339	arg1	hydrogel					379:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	6	41	theme	spiked	1120:1125	arg1	Pb2+					1127:1130	spiked Pb2+	1120:1130	spiked Pb2+ in water and drinking samples	1120:1160	Finally, the method was successfully verified by analyzing spiked Pb2+ in water and drinking samples.					
29622193	1	42	theme	phase	236:240	arg1	extraction					242:251	solid phase extraction	230:251	solid phase extraction of Pb(II) in food and water samples	230:287	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	1	43	theme	hydroxides	341:350	arg1	hydrogel					379:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	3	44	theme	interfering	723:733	arg1	ions					735:738	interfering ions	723:738	interfering ions	723:738	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	1	45	theme	precise	192:198	arg1	method					214:219	an accurate and precise determination method	176:219	an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples	176:287	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	0	46	theme	double	44:49	arg1	hydrogel					70:77	layered double hydroxide-alginate hydrogel	36:77	layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples	36:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	1	47	theme	food	266:269	arg1	samples					281:287	food and water samples	266:287	food and water samples	266:287	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	0	48	theme	water	141:145	arg1	samples					147:153	food and water samples	132:153	food and water samples	132:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	2	49	theme	framework	448:456	arg1	advantages					424:433	the advantages	420:433	the advantages of porous 3D framework of hydrogel	420:468	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
29622193	0	50	theme	layered	36:42	arg1	hydrogel					70:77	layered double hydroxide-alginate hydrogel	36:77	layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples	36:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	3	51	theme	sample	704:709	arg1	volume					711:716	sample volume	704:716	sample volume	704:716	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	1	52	from	extraction	242:251	arg1	samples					281:287	food and water samples	266:287	food and water samples	266:287	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	1	53	theme	LDHs	353:356	arg1	hydrogel					379:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	4	54	theme	experiment	816:825	arg1	conditions					827:836	the optimal experiment conditions	804:836	the optimal experiment conditions of method	804:846	Under the optimal experiment conditions of method, the limit of detection, preconcentration factor and precision as RSD% are found to be 0.39 μg L-1, 53.7 and 2.65%, respectively.					
29622193	1	55	theme	water	275:279	arg1	samples					281:287	food and water samples	266:287	food and water samples	266:287	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	0	56	from	extraction	105:114	arg1	samples					147:153	food and water samples	132:153	food and water samples	132:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	1	57	theme	determination	200:212	arg1	method					214:219	an accurate and precise determination method	176:219	an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples	176:287	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	3	58	theme	volume	711:716	arg1	Effects					633:639	Effects	633:639	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes	633:772	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	1	59	theme	nanosheets-alginate	359:377	arg1	hydrogel					379:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	layered double hydroxides (LDHs) nanosheets-alginate hydrogel	326:386	In order to develop an accurate and precise determination method based on solid phase extraction of Pb(II) in food and water samples, a hybrid monolithic column based on layered double hydroxides (LDHs) nanosheets-alginate hydrogel has been synthesized.					
29622193	0	60	from	ions	124:127	arg1	samples					147:153	food and water samples	132:153	food and water samples	132:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	0	61	theme	hydroxide-alginate	51:68	arg1	hydrogel					70:77	layered double hydroxide-alginate hydrogel	36:77	layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples	36:153	A hybrid monolithic column based on layered double hydroxide-alginate hydrogel for selective solid phase extraction of lead ions in food and water samples.					
29622193	3	62	theme	composition	653:663	arg1	Effects					633:639	Effects	633:639	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes	633:772	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	3	63	theme	ions	735:738	arg1	Effects					633:639	Effects	633:639	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes	633:772	Effects of hydrogel composition, pH, concentration and type of eluent, sample volume, and interfering ions on the recoveries of the analytes were also investigated.					
29622193	2	64	theme	selective	475:483	arg1	adsorption					485:494	selective adsorption	475:494	selective adsorption of LDHs toward Pb(II)	475:516	Combining the advantages of porous 3D framework of hydrogel with selective adsorption of LDHs toward Pb(II), the hydrogel-based hybrid monolithic column shows enhanced enrichment selectivity and efficiency for target ions.					
31769063	9	0	theme	LPS	1672:1674	arg1	levels					1676:1681	LPS levels	1672:1681	LPS levels	1672:1681	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	3	1	theme	protective	541:550	arg1	effects					552:558	the potential protective effects	527:558	the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury	527:628	In this study, we aimed to investigate the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury.					
31769063	9	2	theme	bacteria	1600:1607	arg1	proportion					1575:1584	the proportion	1571:1584	the proportion of beneficial bacteria	1571:1607	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	10	3	theme	inflammatory	1768:1779	arg1	interleukin-6					1830:1842	interleukin-6	1830:1842	interleukin-6	1830:1842	Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated.					
31769063	10	3	theme	inflammatory	1768:1779	arg1	factor					1817:1822	tumor necrosis factor α	1802:1824	tumor necrosis factor α	1802:1824	Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated.					
31769063	10	3	theme	inflammatory	1768:1779	arg1	cytokines					1781:1789	corresponding inflammatory cytokines	1754:1789	corresponding inflammatory cytokines	1754:1789	Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated.					
31769063	9	4	theme	TLR4/Myd88/NF-κB	1698:1713	arg1	activation					1723:1732	TLR4/Myd88/NF-κB pathway activation	1698:1732	TLR4/Myd88/NF-κB pathway activation	1698:1732	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	11	5	theme	radiation	2067:2075	arg1	countermeasure					2077:2090	a promising natural radiation countermeasure	2047:2090	a promising natural radiation countermeasure	2047:2090	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	11	5	theme	radiation	2067:2075	arg1	PC					2041:2042	PC	2041:2042	PC	2041:2042	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	6	6	theme	total-abdominal	1230:1244	arg1	irradiation					1252:1262	total-abdominal x-ray irradiation	1230:1262	total-abdominal x-ray irradiation	1230:1262	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	3	7	theme	intestinal	612:621	arg1	injury					623:628	radiation-induced acute intestinal injury	588:628	radiation-induced acute intestinal injury	588:628	In this study, we aimed to investigate the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury.					
31769063	0	8	theme	TLR4/Myd88/NF-κB	122:137	arg1	Pathway					139:145	the TLR4/Myd88/NF-κB Pathway	118:145	the TLR4/Myd88/NF-κB Pathway	118:145	Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-κB Pathway.					
31769063	10	9	theme	corresponding	1754:1766	arg1	interleukin-6					1830:1842	interleukin-6	1830:1842	interleukin-6	1830:1842	Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated.					
31769063	10	9	theme	corresponding	1754:1766	arg1	factor					1817:1822	tumor necrosis factor α	1802:1824	tumor necrosis factor α	1802:1824	Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated.					
31769063	10	9	theme	corresponding	1754:1766	arg1	cytokines					1781:1789	corresponding inflammatory cytokines	1754:1789	corresponding inflammatory cytokines	1754:1789	Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated.					
31769063	5	10	theme	intestinal	829:838	arg1	histopathology					840:853	intestinal histopathology	829:853	intestinal histopathology	829:853	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	7	11	dep	proteins	1371:1378	arg1	occludens-1					1412:1422	zonula occludens-1	1405:1422	zonula occludens-1(ZO-1)	1405:1428	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	11	dep	proteins	1371:1378	arg1	proteins					1371:1378	the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1)	1352:1428	the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1)	1352:1428	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	11	dep	proteins	1371:1378	arg1	Occludin					1391:1398	Occludin	1391:1398	Occludin	1391:1398	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	11	dep	proteins	1371:1378	arg1	ZO-1					1424:1427	ZO-1	1424:1427	ZO-1	1424:1427	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	11	dep	proteins	1371:1378	arg1	Claudin-1					1380:1388	Claudin-1	1380:1388	Claudin-1	1380:1388	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	5	12	theme	NF-κB	1030:1034	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	8	13	theme	intestinal	1491:1500	arg1	injury					1502:1507	radiation-induced intestinal injury	1473:1507	radiation-induced intestinal injury	1473:1507	PC pretreatment significantly ameliorated radiation-induced intestinal injury.					
31769063	7	14	theme	tight-junction	1356:1369	arg1	occludens-1					1412:1422	zonula occludens-1	1405:1422	zonula occludens-1(ZO-1)	1405:1428	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	14	theme	tight-junction	1356:1369	arg1	proteins					1371:1378	the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1)	1352:1428	the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1)	1352:1428	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	14	theme	tight-junction	1356:1369	arg1	Occludin					1391:1398	Occludin	1391:1398	Occludin	1391:1398	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	14	theme	tight-junction	1356:1369	arg1	Claudin-1					1380:1388	Claudin-1	1380:1388	Claudin-1	1380:1388	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	4	15	theme	x-ray	763:767	arg1	irradiation					769:779	total-abdominal x-ray irradiation	747:779	total-abdominal x-ray irradiation	747:779	MATERIALS AND METHODS C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy.					
31769063	11	16	theme	promising	2049:2057	arg1	countermeasure					2077:2090	a promising natural radiation countermeasure	2047:2090	a promising natural radiation countermeasure	2047:2090	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	11	16	theme	promising	2049:2057	arg1	PC					2041:2042	PC	2041:2042	PC	2041:2042	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	5	17	theme	Toll-like	941:949	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	11	18	from	effect	1968:1973	arg1	pathway					2021:2027	the TLR4/Myd88/NF-κB pathway	2000:2027	the TLR4/Myd88/NF-κB pathway	2000:2027	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	5	19	from	effects	812:818	arg1	integrity					859:867	integrity	859:867	integrity	859:867	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	5	19	from	effects	812:818	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	5	19	from	effects	812:818	arg1	LPS					907:909	LPS	907:909	LPS	907:909	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	5	19	from	effects	812:818	arg1	microbiota					874:883	gut microbiota	870:883	gut microbiota	870:883	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	5	19	from	effects	812:818	arg1	histopathology					840:853	intestinal histopathology	829:853	intestinal histopathology	829:853	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	5	19	from	effects	812:818	arg1	lipopolysaccharides					886:904	lipopolysaccharides	886:904	lipopolysaccharides (LPS)	886:910	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	5	19	from	effects	812:818	arg1	cytokines					926:934	inflammatory cytokines	913:934	inflammatory cytokines	913:934	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	1	20	theme	gastrointestinal	177:192	arg1	syndrome					194:201	BACKGROUND Radiation-induced gastrointestinal syndrome	148:201	BACKGROUND Radiation-induced gastrointestinal syndrome	148:201	BACKGROUND Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment.					
31769063	1	21	theme	life	379:382	arg1	quality					368:374	patient quality	360:374	patient quality of life	360:382	BACKGROUND Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment.					
31769063	5	22	theme	inflammatory	913:924	arg1	cytokines					926:934	inflammatory cytokines	913:934	inflammatory cytokines	913:934	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	0	23	theme	Gut	100:102	arg1	Microbiota					104:113	the Gut Microbiota	96:113	the Gut Microbiota	96:113	Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-κB Pathway.					
31769063	10	24	theme	necrosis	1808:1815	arg1	factor					1817:1822	tumor necrosis factor α	1802:1824	tumor necrosis factor α	1802:1824	Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated.					
31769063	5	25	theme	/myeloid	968:975	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	9	26	theme	gut	1532:1534	arg1	composition					1547:1557	the gut microbiota composition	1528:1557	the gut microbiota composition	1528:1557	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	4	27	theme	Gy	804:805	arg1	dose					793:796	a single dose	784:796	a single dose of 12 Gy	784:805	MATERIALS AND METHODS C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy.					
31769063	5	28	theme	factor	993:998	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	6	29	theme	RESULTS	1063:1069	arg1	damage					1096:1101	RESULTS Severe histopathological damage	1063:1101	RESULTS Severe histopathological damage	1063:1101	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	1	30	theme	BACKGROUND	148:157	arg1	syndrome					194:201	BACKGROUND Radiation-induced gastrointestinal syndrome	148:201	BACKGROUND Radiation-induced gastrointestinal syndrome	148:201	BACKGROUND Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment.					
31769063	2	31	theme	effective	413:421	arg1	countermeasure					433:446	No safe and effective radiation countermeasure	401:446	No safe and effective radiation countermeasure	401:446	No safe and effective radiation countermeasure has been approved for clinical therapy.					
31769063	1	32	theme	industrial	305:314	arg1	exposure					326:333	medical or industrial radiation exposure	294:333	medical or industrial radiation exposure	294:333	BACKGROUND Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment.					
31769063	4	33	theme	single	786:791	arg1	dose					793:796	a single dose	784:796	a single dose of 12 Gy	784:805	MATERIALS AND METHODS C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy.					
31769063	0	34	theme	Radiation-Induced	24:40	arg1	Toxicity					59:66	Radiation-Induced Acute Intestinal Toxicity	24:66	Radiation-Induced Acute Intestinal Toxicity	24:66	Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-κB Pathway.					
31769063	10	35	theme	tumor	1802:1806	arg1	factor					1817:1822	tumor necrosis factor α	1802:1824	tumor necrosis factor α	1802:1824	Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated.					
31769063	5	36	theme	TLR4	963:966	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	9	37	theme	microbiota	1536:1545	arg1	composition					1547:1557	the gut microbiota composition	1528:1557	the gut microbiota composition	1528:1557	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	11	38	theme	gut	1982:1984	arg1	microbiota					1986:1995	the gut microbiota	1978:1995	the gut microbiota	1978:1995	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	0	39	theme	Intestinal	48:57	arg1	Toxicity					59:66	Radiation-Induced Acute Intestinal Toxicity	24:66	Radiation-Induced Acute Intestinal Toxicity	24:66	Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-κB Pathway.					
31769063	5	40	theme	factor	1019:1024	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	6	41	dep	such	1104:1107	arg1	as					1109:1110	as	1109:1110	as	1109:1110	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	6	42	theme	mucosal	1123:1129	arg1	apoptosis					1147:1155	intestinal mucosal epithelial cell apoptosis	1112:1155	intestinal mucosal epithelial cell apoptosis	1112:1155	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	11	43	theme	high-dose	1930:1938	arg1	radiation					1940:1948	high-dose radiation	1930:1948	high-dose radiation	1930:1948	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	9	44	theme	harmful	1632:1638	arg1	bacteria					1640:1647	harmful bacteria	1632:1647	harmful bacteria	1632:1647	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	11	45	theme	mouse	1901:1905	arg1	injury					1918:1923	mouse intestinal injury	1901:1923	mouse intestinal injury	1901:1923	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	4	46	theme	METHODS	645:651	arg1	mice					661:664	MATERIALS AND METHODS C57BL/6 mice	631:664	MATERIALS AND METHODS C57BL/6 mice	631:664	MATERIALS AND METHODS C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy.					
31769063	8	47	theme	PC	1431:1432	arg1	pretreatment					1434:1445	PC pretreatment	1431:1445	PC pretreatment	1431:1445	PC pretreatment significantly ameliorated radiation-induced intestinal injury.					
31769063	2	48	theme	safe	404:407	arg1	countermeasure					433:446	No safe and effective radiation countermeasure	401:446	No safe and effective radiation countermeasure	401:446	No safe and effective radiation countermeasure has been approved for clinical therapy.					
31769063	3	49	theme	potential	531:539	arg1	effects					552:558	the potential protective effects	527:558	the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury	527:628	In this study, we aimed to investigate the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury.					
31769063	6	50	theme	histopathological	1078:1094	arg1	damage					1096:1101	RESULTS Severe histopathological damage	1063:1101	RESULTS Severe histopathological damage	1063:1101	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	1	51	theme	medical	294:300	arg1	exposure					326:333	medical or industrial radiation exposure	294:333	medical or industrial radiation exposure	294:333	BACKGROUND Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment.					
31769063	6	52	theme	nuclear	1172:1178	arg1	rupture					1180:1186	nuclear rupture	1172:1186	nuclear rupture	1172:1186	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	4	53	theme	MATERIALS	631:639	arg1	mice					661:664	MATERIALS AND METHODS C57BL/6 mice	631:664	MATERIALS AND METHODS C57BL/6 mice	631:664	MATERIALS AND METHODS C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy.					
31769063	3	54	theme	phycocyanin	563:573	arg1	effects					552:558	the potential protective effects	527:558	the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury	527:628	In this study, we aimed to investigate the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury.					
31769063	9	55	theme	pathway	1715:1721	arg1	activation					1723:1732	TLR4/Myd88/NF-κB pathway activation	1698:1732	TLR4/Myd88/NF-κB pathway activation	1698:1732	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	4	56	theme	PC	700:701	arg1	mg/kg					694:698	50 mg/kg PC	691:701	50 mg/kg PC once per day for 1 month	691:726	MATERIALS AND METHODS C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy.					
31769063	6	57	theme	x-ray	1246:1250	arg1	irradiation					1252:1262	total-abdominal x-ray irradiation	1230:1262	total-abdominal x-ray irradiation	1230:1262	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	3	58	theme	acute	606:610	arg1	injury					623:628	radiation-induced acute intestinal injury	588:628	radiation-induced acute intestinal injury	588:628	In this study, we aimed to investigate the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury.					
31769063	6	59	theme	cell	1142:1145	arg1	apoptosis					1147:1155	intestinal mucosal epithelial cell apoptosis	1112:1155	intestinal mucosal epithelial cell apoptosis	1112:1155	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	11	60	theme	natural	2059:2065	arg1	countermeasure					2077:2090	a promising natural radiation countermeasure	2047:2090	a promising natural radiation countermeasure	2047:2090	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	11	60	theme	natural	2059:2065	arg1	PC					2041:2042	PC	2041:2042	PC	2041:2042	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	0	61	from	Effect	86:91	arg1	Pathway					139:145	the TLR4/Myd88/NF-κB Pathway	118:145	the TLR4/Myd88/NF-κB Pathway	118:145	Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-κB Pathway.					
31769063	11	62	theme	TLR4/Myd88/NF-κB	2004:2019	arg1	pathway					2021:2027	the TLR4/Myd88/NF-κB pathway	2000:2027	the TLR4/Myd88/NF-κB pathway	2000:2027	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	7	63	theme	zonula	1405:1410	arg1	occludens-1					1412:1422	zonula occludens-1	1405:1422	zonula occludens-1(ZO-1)	1405:1428	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	63	theme	zonula	1405:1410	arg1	proteins					1371:1378	the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1)	1352:1428	the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1)	1352:1428	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	63	theme	zonula	1405:1410	arg1	ZO-1					1424:1427	ZO-1	1424:1427	ZO-1	1424:1427	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	7	64	theme	proteins	1371:1378	arg1	levels					1342:1347	reduced levels	1334:1347	reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1)	1334:1428	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	4	65	theme	total-abdominal	747:761	arg1	irradiation					769:779	total-abdominal x-ray irradiation	747:779	total-abdominal x-ray irradiation	747:779	MATERIALS AND METHODS C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy.					
31769063	7	66	theme	Intestinal	1265:1274	arg1	integrity					1276:1284	Intestinal integrity	1265:1284	Intestinal integrity	1265:1284	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	1	67	theme	Radiation-induced	159:175	arg1	syndrome					194:201	BACKGROUND Radiation-induced gastrointestinal syndrome	148:201	BACKGROUND Radiation-induced gastrointestinal syndrome	148:201	BACKGROUND Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment.					
31769063	6	68	dep	hours	1218:1222	arg1	irradiation					1252:1262	total-abdominal x-ray irradiation	1230:1262	total-abdominal x-ray irradiation	1230:1262	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	0	69	theme	Microbiota	104:113	arg1	Effect					86:91	the Effect	82:91	the Effect of the Gut Microbiota on the TLR4/Myd88/NF-κB Pathway	82:145	Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-κB Pathway.					
31769063	3	70	theme	radiation-induced	588:604	arg1	injury					623:628	radiation-induced acute intestinal injury	588:628	radiation-induced acute intestinal injury	588:628	In this study, we aimed to investigate the potential protective effects of phycocyanin (PC) against radiation-induced acute intestinal injury.					
31769063	5	71	theme	differentiation	977:991	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	7	72	theme	reduced	1334:1340	arg1	levels					1342:1347	reduced levels	1334:1347	reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1)	1334:1428	Intestinal integrity was damaged by irradiation, which manifested in reduced levels of the tight-junction proteins Claudin-1, Occludin, and zonula occludens-1(ZO-1).					
31769063	9	73	dep	modulated	1518:1526	arg1	lowered					1664:1670	lowered	1664:1670	lowered LPS levels	1664:1681	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	9	73	dep	modulated	1518:1526	arg1	decreasing					1613:1622	decreasing	1613:1622	decreasing that of harmful bacteria	1613:1647	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	9	73	dep	modulated	1518:1526	arg1	suppressed					1687:1696	suppressed	1687:1696	suppressed TLR4/Myd88/NF-κB pathway activation	1687:1732	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	9	73	dep	modulated	1518:1526	arg1	increasing					1560:1569	increasing	1560:1569	increasing the proportion of beneficial bacteria	1560:1607	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	2	74	theme	radiation	423:431	arg1	countermeasure					433:446	No safe and effective radiation countermeasure	401:446	No safe and effective radiation countermeasure	401:446	No safe and effective radiation countermeasure has been approved for clinical therapy.					
31769063	5	75	theme	PC	823:824	arg1	effects					812:818	The effects	808:818	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	808:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	5	76	theme	receptor	951:958	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	0	77	theme	Acute	42:46	arg1	Toxicity					59:66	Radiation-Induced Acute Intestinal Toxicity	24:66	Radiation-Induced Acute Intestinal Toxicity	24:66	Phycocyanin Ameliorates Radiation-Induced Acute Intestinal Toxicity by Regulating the Effect of the Gut Microbiota on the TLR4/Myd88/NF-κB Pathway.					
31769063	1	78	theme	radiation	316:324	arg1	exposure					326:333	medical or industrial radiation exposure	294:333	medical or industrial radiation exposure	294:333	BACKGROUND Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment.					
31769063	2	79	theme	clinical	470:477	arg1	therapy					479:485	clinical therapy	470:485	clinical therapy	470:485	No safe and effective radiation countermeasure has been approved for clinical therapy.					
31769063	5	80	theme	/nuclear	1010:1017	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	6	81	theme	Severe	1071:1076	arg1	damage					1096:1101	RESULTS Severe histopathological damage	1063:1101	RESULTS Severe histopathological damage	1063:1101	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	11	82	theme	microbiota	1986:1995	arg1	effect					1968:1973	the effect	1964:1973	the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway	1964:2027	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	5	83	theme	κB	1026:1027	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	6	84	theme	epithelial	1131:1140	arg1	apoptosis					1147:1155	intestinal mucosal epithelial cell apoptosis	1112:1155	intestinal mucosal epithelial cell apoptosis	1112:1155	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	8	85	theme	radiation-induced	1473:1489	arg1	injury					1502:1507	radiation-induced intestinal injury	1473:1507	radiation-induced intestinal injury	1473:1507	PC pretreatment significantly ameliorated radiation-induced intestinal injury.					
31769063	1	86	theme	patient	360:366	arg1	quality					368:374	patient quality	360:374	patient quality of life	360:382	BACKGROUND Radiation-induced gastrointestinal syndrome, including nausea, diarrhea, and dehydration, contributes to morbidity and mortality after medical or industrial radiation exposure, which seriously affects patient quality of life after treatment.					
31769063	6	87	theme	intestinal	1112:1121	arg1	apoptosis					1147:1155	intestinal mucosal epithelial cell apoptosis	1112:1155	intestinal mucosal epithelial cell apoptosis	1112:1155	RESULTS Severe histopathological damage, such as intestinal mucosal epithelial cell apoptosis, necrosis, and nuclear rupture, was most clearly observed 24 hours after total-abdominal x-ray irradiation.					
31769063	4	88	theme	C57BL/6	653:659	arg1	mice					661:664	MATERIALS AND METHODS C57BL/6 mice	631:664	MATERIALS AND METHODS C57BL/6 mice	631:664	MATERIALS AND METHODS C57BL/6 mice were orally administered 50 mg/kg PC once per day for 1 month before exposure to total-abdominal x-ray irradiation at a single dose of 12 Gy.					
31769063	9	89	theme	beneficial	1589:1598	arg1	bacteria					1600:1607	beneficial bacteria	1589:1607	beneficial bacteria	1589:1607	PC also modulated the gut microbiota composition, increasing the proportion of beneficial bacteria and decreasing that of harmful bacteria, which in turn lowered LPS levels and suppressed TLR4/Myd88/NF-κB pathway activation.					
31769063	11	90	theme	intestinal	1907:1916	arg1	injury					1918:1923	mouse intestinal injury	1901:1923	mouse intestinal injury	1901:1923	CONCLUSION PC protects against mouse intestinal injury from high-dose radiation by regulating the effect of the gut microbiota on the TLR4/Myd88/NF-κB pathway, suggesting PC as a promising natural radiation countermeasure.					
31769063	5	91	theme	gut	870:872	arg1	microbiota					874:883	gut microbiota	870:883	gut microbiota	870:883	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	5	92	theme	Myd88	1004:1008	arg1	signaling					1037:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling	941:1045	The effects of PC on intestinal histopathology and integrity, gut microbiota, lipopolysaccharides (LPS), inflammatory cytokines, and Toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (Myd88)/nuclear factor κB (NF-κB) signaling were evaluated.					
31769063	10	93	theme	cytokines	1781:1789	arg1	levels					1744:1749	levels	1744:1749	levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6,	1744:1843	Finally, levels of corresponding inflammatory cytokines, including tumor necrosis factor α and interleukin-6, were also downregulated.					
30796749	2	0	dep	anaerobic	148:156	arg1	Gram-stain-positive					159:177	Gram-stain-positive	159:177	Gram-stain-positive	159:177	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102T, was isolated from the small intestine of a swine.					
30796749	2	0	dep	anaerobic	148:156	arg1	rod-shaped					211:220	rod-shaped	211:220	rod-shaped	211:220	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102T, was isolated from the small intestine of a swine.					
30796749	2	0	dep	anaerobic	148:156	arg1	non-motile					199:208	non-motile	199:208	non-motile	199:208	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102T, was isolated from the small intestine of a swine.					
30796749	2	0	dep	anaerobic	148:156	arg1	catalase-negative					180:196	catalase-negative	180:196	catalase-negative	180:196	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102T, was isolated from the small intestine of a swine.					
30796749	12	1	theme	phenotypic	1500:1509	arg1	properties					1528:1537	These distinct phenotypic and phylogenetic properties	1485:1537	These distinct phenotypic and phylogenetic properties	1485:1537	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	5	2	theme	strain	526:531	arg1	SG0102T					533:539	strain SG0102T	526:539	strain SG0102T	526:539	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	0	3	theme	Intestinibaculum	0:15	arg1	nov.					28:31	Intestinibaculum porci gen. nov.	0:31	Intestinibaculum porci gen. nov.	0:31	Intestinibaculum porci gen. nov., sp.					
30796749	12	4	from	species	1586:1592	arg1	genus					1605:1609	a novel genus	1597:1609	a novel genus of the family Erysipelotrichaceae	1597:1643	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	5	5	theme	catenaformis	720:731	arg1	5348T					737:741	Eggerthia catenaformis DSM 5348T	710:741	Eggerthia catenaformis DSM 5348T (89.6%)	710:749	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	5	theme	catenaformis	720:731	arg1	%					748:748	89.6%	744:748	89.6%	744:748	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	4	6	located	observed	371:378	arg1	presence					387:394	the presence	383:394	the presence of up to 3% (w/v) NaCl	383:417	Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%.					
30796749	4	6	located	observed	371:378	arg2	growth					360:365	growth	360:365	growth	360:365	Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%.					
30796749	9	7	theme	cellular	1220:1227	arg1	%					1246:1246	> 10%	1242:1246	> 10%	1242:1246	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	9	7	theme	cellular	1220:1227	arg1	C16:0					1272:1276	C16:0	1272:1276	C16:0	1272:1276	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	9	7	theme	cellular	1220:1227	arg1	acids					1235:1239	The major cellular fatty acids	1210:1239	The major cellular fatty acids (> 10%) of strain SG0102T	1210:1265	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	12	8	theme	gen.	1688:1691	arg1	nov.					1693:1696	Intestinibaculum porci gen. nov.	1665:1696	the name Intestinibaculum porci gen. nov.	1656:1696	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	8	9	theme	mol	1204:1206	arg1	G					1159:1159	The DNA G + C content	1151:1171	G	1159:1159	The DNA G + C content of the strain SG0102T was 39.5 mol%.					
30796749	8	9	theme	mol	1204:1206	arg1	%					1207:1207	39.5 mol%	1199:1207	39.5 mol%	1199:1207	The DNA G + C content of the strain SG0102T was 39.5 mol%.					
30796749	12	10	dep	name	1660:1663	arg1	nov.					1693:1696	Intestinibaculum porci gen. nov.	1665:1696	the name Intestinibaculum porci gen. nov.	1656:1696	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	6	11	theme	identity	775:782	arg1	values					784:789	The average nucleotide identity values	752:789	The average nucleotide identity values	752:789	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	6	11	theme	identity	775:782	arg1	%					973:973	71.0, 69.3, 70.0, and 69.2%	947:973	71.0, 69.3, 70.0, and 69.2%	947:973	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	10	12	theme	wall	1328:1331	arg1	peptidoglycan					1333:1345	The cell wall peptidoglycan	1319:1345	The cell wall peptidoglycan of strain SG0102T	1319:1363	The cell wall peptidoglycan of strain SG0102T contained the meso-diaminopimelic acid.					
30796749	5	13	theme	vitulina	579:586	arg1	20405T					592:597	Kandleria vitulina DSM 20405T	569:597	Kandleria vitulina DSM 20405T (93.3%)	569:605	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	13	theme	vitulina	579:586	arg1	%					604:604	93.3%	600:604	93.3%	600:604	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	9	14	theme	>	1242:1242	arg1	%					1246:1246	> 10%	1242:1246	> 10%	1242:1246	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	9	14	theme	>	1242:1242	arg1	acids					1235:1239	The major cellular fatty acids	1210:1239	The major cellular fatty acids (> 10%) of strain SG0102T	1210:1265	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	6	15	theme	average	756:762	arg1	values					784:789	The average nucleotide identity values	752:789	The average nucleotide identity values	752:789	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	6	15	theme	average	756:762	arg1	%					973:973	71.0, 69.3, 70.0, and 69.2%	947:973	71.0, 69.3, 70.0, and 69.2%	947:973	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	11	16	theme	fermentation	1471:1482	arg1	product					1460:1466	a major end product	1448:1466	a major end product of fermentation	1448:1482	The strain SG0102T produced lactic acid as a major end product of fermentation.					
30796749	11	16	theme	fermentation	1471:1482	arg1	acid					1440:1443	lactic acid	1433:1443	lactic acid	1433:1443	The strain SG0102T produced lactic acid as a major end product of fermentation.					
30796749	1	17	theme	small	109:113	arg1	intestine					115:123	the small intestine	105:123	the small intestine of a swine	105:134	nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.					
30796749	5	18	theme	KCTC	646:649	arg1	5053T					651:655	Catenibacterium mitsuokai KCTC 5053T	620:655	Catenibacterium mitsuokai KCTC 5053T (91.1%)	620:663	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	18	theme	KCTC	646:649	arg1	%					662:662	91.1%	658:662	91.1%	658:662	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	6	19	theme	vitulina	838:845	arg1	20405T					851:856	K. vitulina DSM 20405T	835:856	K. vitulina DSM 20405T	835:856	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	4	20	theme	NaCl	414:417	arg1	presence					387:394	the presence	383:394	the presence of up to 3% (w/v) NaCl	383:417	Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%.					
30796749	12	21	dep	sp	1698:1699	arg1	name					1660:1663	the name	1656:1663	the name Intestinibaculum porci gen. nov.	1656:1696	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	5	22	theme	rRNA	494:497	arg1	sequences					504:512	16S rRNA gene sequences	490:512	16S rRNA gene sequences	490:512	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	1	23	theme	swine	130:134	arg1	intestine					115:123	the small intestine	105:123	the small intestine of a swine	105:134	nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.					
30796749	11	24	theme	lactic	1433:1438	arg1	product					1460:1466	a major end product	1448:1466	a major end product of fermentation	1448:1482	The strain SG0102T produced lactic acid as a major end product of fermentation.					
30796749	11	24	theme	lactic	1433:1438	arg1	acid					1440:1443	lactic acid	1433:1443	lactic acid	1433:1443	The strain SG0102T produced lactic acid as a major end product of fermentation.					
30796749	5	25	theme	sequences	504:512	arg1	analysis					478:485	The comparative analysis	462:485	The comparative analysis of 16S rRNA gene sequences	462:512	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	6	26	theme	related	818:824	arg1	species					826:832	related species	818:832	related species	818:832	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	10	27	theme	meso-diaminopimelic	1379:1397	arg1	acid					1399:1402	the meso-diaminopimelic acid	1375:1402	the meso-diaminopimelic acid	1375:1402	The cell wall peptidoglycan of strain SG0102T contained the meso-diaminopimelic acid.					
30796749	5	28	theme	Catenibacterium	620:634	arg1	5053T					651:655	Catenibacterium mitsuokai KCTC 5053T	620:655	Catenibacterium mitsuokai KCTC 5053T (91.1%)	620:663	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	28	theme	Catenibacterium	620:634	arg1	%					662:662	91.1%	658:662	91.1%	658:662	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	12	29	theme	family	1618:1623	arg1	Erysipelotrichaceae					1625:1643	the family Erysipelotrichaceae	1614:1643	the family Erysipelotrichaceae	1614:1643	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	5	30	theme	comparative	466:476	arg1	analysis					478:485	The comparative analysis	462:485	The comparative analysis of 16S rRNA gene sequences	462:512	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	9	31	theme	C16:0	1279:1283	arg1	acetal					1294:1299	C16:0 dimethyl acetal	1279:1299	C16:0 dimethyl acetal	1279:1299	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	6	32	theme	azabuensis	887:896	arg1	15217T					903:908	S. azabuensis KCTC 15217T	884:908	S. azabuensis KCTC 15217T	884:908	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	5	33	dep	related	558:564	arg1	followed					608:615	followed	608:615	followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%)	608:749	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	34	theme	mitsuokai	636:644	arg1	5053T					651:655	Catenibacterium mitsuokai KCTC 5053T	620:655	Catenibacterium mitsuokai KCTC 5053T (91.1%)	620:663	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	34	theme	mitsuokai	636:644	arg1	%					662:662	91.1%	658:662	91.1%	658:662	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	8	35	theme	DNA	1155:1157	arg1	G					1159:1159	The DNA G + C content	1151:1171	G	1159:1159	The DNA G + C content of the strain SG0102T was 39.5 mol%.					
30796749	8	35	theme	DNA	1155:1157	arg1	%					1207:1207	39.5 mol%	1199:1207	39.5 mol%	1199:1207	The DNA G + C content of the strain SG0102T was 39.5 mol%.					
30796749	5	36	theme	azabuensis	674:683	arg1	%					702:702	91.0%	698:702	91.0%	698:702	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	36	theme	azabuensis	674:683	arg1	15217T					690:695	Sharpea azabuensis KCTC 15217T	666:695	Sharpea azabuensis KCTC 15217T (91.0%)	666:703	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	14	37	theme	type	1724:1727	arg1	SG0102T					1739:1745	SG0102T	1739:1745	SG0102T (= KCTC 15725T = NBRC 113396T)	1739:1776	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	14	37	theme	type	1724:1727	arg1	strain					1729:1734	The type strain	1720:1734	The type strain	1720:1734	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	7	38	theme	family	1093:1098	arg1	Erysipelotrichaceae					1100:1118	the family Erysipelotrichaceae	1089:1118	the family Erysipelotrichaceae in the class Erysipelotrichia	1089:1148	The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102T belonged to the family Erysipelotrichaceae in the class Erysipelotrichia.					
30796749	0	39	theme	gen.	23:26	arg1	nov.					28:31	Intestinibaculum porci gen. nov.	0:31	Intestinibaculum porci gen. nov.	0:31	Intestinibaculum porci gen. nov., sp.					
30796749	12	40	theme	novel	1599:1603	arg1	genus					1605:1609	a novel genus	1597:1609	a novel genus of the family Erysipelotrichaceae	1597:1643	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	1	41	theme	Erysipelotrichaceae	71:89	arg1	member					50:55	a new member	44:55	a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine	44:134	nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.					
30796749	1	41	theme	Erysipelotrichaceae	71:89	arg1	nov.					38:41	nov.	38:41	nov.	38:41	nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.					
30796749	6	42	theme	KCTC	872:875	arg1	5053T					877:881	C. mitsuokai KCTC 5053T	859:881	C. mitsuokai KCTC 5053T	859:881	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	6	43	theme	mitsuokai	862:870	arg1	5053T					877:881	C. mitsuokai KCTC 5053T	859:881	C. mitsuokai KCTC 5053T	859:881	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	6	44	theme	C.	859:860	arg1	5053T					877:881	C. mitsuokai KCTC 5053T	859:881	C. mitsuokai KCTC 5053T	859:881	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	4	45	theme	salinity	430:437	arg1	levels					439:444	salinity levels	430:444	salinity levels higher than 4%	430:459	Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%.					
30796749	14	46	theme	NBRC	1764:1767	arg1	SG0102T					1739:1745	SG0102T	1739:1745	SG0102T (= KCTC 15725T = NBRC 113396T)	1739:1776	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	14	46	theme	NBRC	1764:1767	arg1	113396T					1769:1775	= KCTC 15725T = NBRC 113396T	1748:1775	= KCTC 15725T = NBRC 113396T	1748:1775	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	12	47	theme	novel	1580:1584	arg1	species					1586:1592	a novel species	1578:1592	a novel species	1578:1592	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	4	48	theme	higher	446:451	arg1	levels					439:444	salinity levels	430:444	salinity levels higher than 4%	430:459	Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%.					
30796749	14	49	theme	15725T	1755:1760	arg1	SG0102T					1739:1745	SG0102T	1739:1745	SG0102T (= KCTC 15725T = NBRC 113396T)	1739:1776	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	14	49	theme	15725T	1755:1760	arg1	113396T					1769:1775	= KCTC 15725T = NBRC 113396T	1748:1775	= KCTC 15725T = NBRC 113396T	1748:1775	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	11	50	theme	end	1456:1458	arg1	product					1460:1466	a major end product	1448:1466	a major end product of fermentation	1448:1482	The strain SG0102T produced lactic acid as a major end product of fermentation.					
30796749	11	50	theme	end	1456:1458	arg1	acid					1440:1443	lactic acid	1433:1443	lactic acid	1433:1443	The strain SG0102T produced lactic acid as a major end product of fermentation.					
30796749	8	51	theme	SG0102T	1187:1193	arg1	G					1159:1159	The DNA G + C content	1151:1171	G	1159:1159	The DNA G + C content of the strain SG0102T was 39.5 mol%.					
30796749	8	51	theme	SG0102T	1187:1193	arg1	%					1207:1207	39.5 mol%	1199:1207	39.5 mol%	1199:1207	The DNA G + C content of the strain SG0102T was 39.5 mol%.					
30796749	2	52	theme	swine	296:300	arg1	intestine					281:289	the small intestine	271:289	the small intestine of a swine	271:300	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102T, was isolated from the small intestine of a swine.					
30796749	6	53	theme	catenaformis	918:929	arg1	5348T					935:939	E. catenaformis DSM 5348T	915:939	E. catenaformis DSM 5348T	915:939	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	7	54	from	Erysipelotrichaceae	1100:1118	arg1	Erysipelotrichia					1133:1148	the class Erysipelotrichia	1123:1148	the class Erysipelotrichia	1123:1148	The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102T belonged to the family Erysipelotrichaceae in the class Erysipelotrichia.					
30796749	7	55	theme	rRNA	1029:1032	arg1	sequence					1039:1046	16S rRNA gene sequence	1025:1046	16S rRNA gene sequence	1025:1046	The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102T belonged to the family Erysipelotrichaceae in the class Erysipelotrichia.					
30796749	1	56	attach	isolated	91:98	arg2	Erysipelotrichaceae					71:89	the family Erysipelotrichaceae	60:89	the family Erysipelotrichaceae isolated from the small intestine of a swine	60:134	nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.					
30796749	1	56	attach	isolated	91:98	arg1	intestine					115:123	the small intestine	105:123	the small intestine of a swine	105:134	nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.					
30796749	4	57	dep	3	405:405	arg1	to					402:403	to	402:403	to	402:403	Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%.					
30796749	10	58	contain	contained	1365:1373	arg1	peptidoglycan					1333:1345	The cell wall peptidoglycan	1319:1345	The cell wall peptidoglycan of strain SG0102T	1319:1363	The cell wall peptidoglycan of strain SG0102T contained the meso-diaminopimelic acid.					
30796749	10	58	contain	contained	1365:1373	arg2	acid					1399:1402	the meso-diaminopimelic acid	1375:1402	the meso-diaminopimelic acid	1375:1402	The cell wall peptidoglycan of strain SG0102T contained the meso-diaminopimelic acid.					
30796749	12	59	theme	distinct	1491:1498	arg1	properties					1528:1537	These distinct phenotypic and phylogenetic properties	1485:1537	These distinct phenotypic and phylogenetic properties	1485:1537	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	11	60	theme	strain	1409:1414	arg1	SG0102T					1416:1422	The strain SG0102T	1405:1422	The strain SG0102T	1405:1422	The strain SG0102T produced lactic acid as a major end product of fermentation.					
30796749	10	61	theme	cell	1323:1326	arg1	peptidoglycan					1333:1345	The cell wall peptidoglycan	1319:1345	The cell wall peptidoglycan of strain SG0102T	1319:1363	The cell wall peptidoglycan of strain SG0102T contained the meso-diaminopimelic acid.					
30796749	5	62	theme	Eggerthia	710:718	arg1	5348T					737:741	Eggerthia catenaformis DSM 5348T	710:741	Eggerthia catenaformis DSM 5348T (89.6%)	710:749	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	62	theme	Eggerthia	710:718	arg1	%					748:748	89.6%	744:748	89.6%	744:748	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	2	63	theme	small	275:279	arg1	intestine					281:289	the small intestine	271:289	the small intestine of a swine	271:300	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102T, was isolated from the small intestine of a swine.					
30796749	9	64	theme	major	1214:1218	arg1	%					1246:1246	> 10%	1242:1246	> 10%	1242:1246	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	9	64	theme	major	1214:1218	arg1	C16:0					1272:1276	C16:0	1272:1276	C16:0	1272:1276	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	9	64	theme	major	1214:1218	arg1	acids					1235:1239	The major cellular fatty acids	1210:1239	The major cellular fatty acids (> 10%) of strain SG0102T	1210:1265	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	6	65	theme	strain	799:804	arg1	SG0102T					806:812	strain SG0102T	799:812	strain SG0102T	799:812	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	9	66	theme	SG0102T	1259:1265	arg1	%					1246:1246	> 10%	1242:1246	> 10%	1242:1246	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	9	66	theme	SG0102T	1259:1265	arg1	C16:0					1272:1276	C16:0	1272:1276	C16:0	1272:1276	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	9	66	theme	SG0102T	1259:1265	arg1	acids					1235:1239	The major cellular fatty acids	1210:1239	The major cellular fatty acids (> 10%) of strain SG0102T	1210:1265	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	10	67	theme	strain	1350:1355	arg1	SG0102T					1357:1363	strain SG0102T	1350:1363	strain SG0102T	1350:1363	The cell wall peptidoglycan of strain SG0102T contained the meso-diaminopimelic acid.					
30796749	9	68	theme	fatty	1229:1233	arg1	%					1246:1246	> 10%	1242:1246	> 10%	1242:1246	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	9	68	theme	fatty	1229:1233	arg1	C16:0					1272:1276	C16:0	1272:1276	C16:0	1272:1276	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	9	68	theme	fatty	1229:1233	arg1	acids					1235:1239	The major cellular fatty acids	1210:1239	The major cellular fatty acids (> 10%) of strain SG0102T	1210:1265	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	12	69	theme	porci	1682:1686	arg1	nov.					1693:1696	Intestinibaculum porci gen. nov.	1665:1696	the name Intestinibaculum porci gen. nov.	1656:1696	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	2	70	attach	isolated	257:264	arg1	intestine					281:289	the small intestine	271:289	the small intestine of a swine	271:300	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102T, was isolated from the small intestine of a swine.					
30796749	2	70	attach	isolated	257:264	arg2	bacterium					222:230	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium	137:230	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium	137:230	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102T, was isolated from the small intestine of a swine.					
30796749	5	71	theme	Kandleria	569:577	arg1	20405T					592:597	Kandleria vitulina DSM 20405T	569:597	Kandleria vitulina DSM 20405T (93.3%)	569:605	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	71	theme	Kandleria	569:577	arg1	%					604:604	93.3%	600:604	93.3%	600:604	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	6	72	theme	nucleotide	764:773	arg1	values					784:789	The average nucleotide identity values	752:789	The average nucleotide identity values	752:789	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	6	72	theme	nucleotide	764:773	arg1	%					973:973	71.0, 69.3, 70.0, and 69.2%	947:973	71.0, 69.3, 70.0, and 69.2%	947:973	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	5	73	theme	DSM	588:590	arg1	20405T					592:597	Kandleria vitulina DSM 20405T	569:597	Kandleria vitulina DSM 20405T (93.3%)	569:605	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	73	theme	DSM	588:590	arg1	%					604:604	93.3%	600:604	93.3%	600:604	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	6	74	theme	DSM	931:933	arg1	5348T					935:939	E. catenaformis DSM 5348T	915:939	E. catenaformis DSM 5348T	915:939	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	7	75	theme	phylogenetic	994:1005	arg1	analysis					1007:1014	The phylogenetic analysis	990:1014	The phylogenetic analysis based on 16S rRNA gene sequence	990:1046	The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102T belonged to the family Erysipelotrichaceae in the class Erysipelotrichia.					
30796749	5	76	theme	DSM	733:735	arg1	5348T					737:741	Eggerthia catenaformis DSM 5348T	710:741	Eggerthia catenaformis DSM 5348T (89.6%)	710:749	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	76	theme	DSM	733:735	arg1	%					748:748	89.6%	744:748	89.6%	744:748	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	6	77	theme	DSM	847:849	arg1	20405T					851:856	K. vitulina DSM 20405T	835:856	K. vitulina DSM 20405T	835:856	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	3	78	theme	Optimal	303:309	arg1	growth					311:316	Optimal growth	303:316	Optimal growth	303:316	Optimal growth occurred at 37°C and pH 7.0.					
30796749	7	79	theme	class	1127:1131	arg1	Erysipelotrichia					1133:1148	the class Erysipelotrichia	1123:1148	the class Erysipelotrichia	1123:1148	The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102T belonged to the family Erysipelotrichaceae in the class Erysipelotrichia.					
30796749	9	80	theme	strain	1252:1257	arg1	SG0102T					1259:1265	strain SG0102T	1252:1265	strain SG0102T	1252:1265	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	6	81	theme	K.	835:836	arg1	20405T					851:856	K. vitulina DSM 20405T	835:856	K. vitulina DSM 20405T	835:856	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	12	82	theme	Intestinibaculum	1665:1680	arg1	nov.					1693:1696	Intestinibaculum porci gen. nov.	1665:1696	the name Intestinibaculum porci gen. nov.	1656:1696	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	5	83	theme	gene	499:502	arg1	sequences					504:512	16S rRNA gene sequences	490:512	16S rRNA gene sequences	490:512	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	14	84	theme	=	1748:1748	arg1	SG0102T					1739:1745	SG0102T	1739:1745	SG0102T (= KCTC 15725T = NBRC 113396T)	1739:1776	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	14	84	theme	=	1748:1748	arg1	113396T					1769:1775	= KCTC 15725T = NBRC 113396T	1748:1775	= KCTC 15725T = NBRC 113396T	1748:1775	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	12	85	theme	Erysipelotrichaceae	1625:1643	arg1	genus					1605:1609	a novel genus	1597:1609	a novel genus of the family Erysipelotrichaceae	1597:1643	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	1	86	theme	new	46:48	arg1	member					50:55	a new member	44:55	a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine	44:134	nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.					
30796749	1	86	theme	new	46:48	arg1	nov.					38:41	nov.	38:41	nov.	38:41	nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.					
30796749	6	87	theme	KCTC	898:901	arg1	15217T					903:908	S. azabuensis KCTC 15217T	884:908	S. azabuensis KCTC 15217T	884:908	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	0	88	theme	porci	17:21	arg1	nov.					28:31	Intestinibaculum porci gen. nov.	0:31	Intestinibaculum porci gen. nov.	0:31	Intestinibaculum porci gen. nov., sp.					
30796749	9	89	theme	dimethyl	1285:1292	arg1	acetal					1294:1299	C16:0 dimethyl acetal	1279:1299	C16:0 dimethyl acetal	1279:1299	The major cellular fatty acids (> 10%) of strain SG0102T were C16:0, C16:0 dimethyl acetal, and C18:2ω9/12c.					
30796749	6	90	theme	S.	884:885	arg1	15217T					903:908	S. azabuensis KCTC 15217T	884:908	S. azabuensis KCTC 15217T	884:908	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	10	91	theme	SG0102T	1357:1363	arg1	peptidoglycan					1333:1345	The cell wall peptidoglycan	1319:1345	The cell wall peptidoglycan of strain SG0102T	1319:1363	The cell wall peptidoglycan of strain SG0102T contained the meso-diaminopimelic acid.					
30796749	5	92	theme	KCTC	685:688	arg1	%					702:702	91.0%	698:702	91.0%	698:702	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	92	theme	KCTC	685:688	arg1	15217T					690:695	Sharpea azabuensis KCTC 15217T	666:695	Sharpea azabuensis KCTC 15217T (91.0%)	666:703	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	93	theme	16S	490:492	arg1	sequences					504:512	16S rRNA gene sequences	490:512	16S rRNA gene sequences	490:512	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	1	94	theme	family	64:69	arg1	Erysipelotrichaceae					71:89	the family Erysipelotrichaceae	60:89	the family Erysipelotrichaceae isolated from the small intestine of a swine	60:134	nov., a new member of the family Erysipelotrichaceae isolated from the small intestine of a swine.					
30796749	2	95	theme	anaerobic	148:156	arg1	bacterium					222:230	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium	137:230	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium	137:230	A strictly anaerobic, Gram-stain-positive, catalase-negative, non-motile, rod-shaped bacterium, designated SG0102T, was isolated from the small intestine of a swine.					
30796749	6	96	theme	E.	915:916	arg1	5348T					935:939	E. catenaformis DSM 5348T	915:939	E. catenaformis DSM 5348T	915:939	The average nucleotide identity values between strain SG0102T and related species, K. vitulina DSM 20405T, C. mitsuokai KCTC 5053T, S. azabuensis KCTC 15217T, and E. catenaformis DSM 5348T, were 71.0, 69.3, 70.0, and 69.2%, respectively.					
30796749	4	97	dep	%	406:406	arg1	w/v					409:411	w/v	409:411	w/v	409:411	Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%.					
30796749	4	97	dep	%	406:406	arg1	3					405:405	3	405:405	3	405:405	Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%.					
30796749	7	98	theme	strain	1062:1067	arg1	SG0102T					1069:1075	strain SG0102T	1062:1075	strain SG0102T	1062:1075	The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102T belonged to the family Erysipelotrichaceae in the class Erysipelotrichia.					
30796749	8	99	theme	C	1163:1163	arg1	content					1165:1171	The DNA G + C content	1151:1171	content	1165:1171	The DNA G + C content of the strain SG0102T was 39.5 mol%.					
30796749	0	100	dep	sp	34:35	arg1	nov.					28:31	Intestinibaculum porci gen. nov.	0:31	Intestinibaculum porci gen. nov.	0:31	Intestinibaculum porci gen. nov., sp.					
30796749	12	101	theme	strain	1552:1557	arg1	SG0102T					1559:1565	strain SG0102T	1552:1565	strain SG0102T	1552:1565	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	14	102	theme	=	1762:1762	arg1	SG0102T					1739:1745	SG0102T	1739:1745	SG0102T (= KCTC 15725T = NBRC 113396T)	1739:1776	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	14	102	theme	=	1762:1762	arg1	113396T					1769:1775	= KCTC 15725T = NBRC 113396T	1748:1775	= KCTC 15725T = NBRC 113396T	1748:1775	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	11	103	theme	major	1450:1454	arg1	product					1460:1466	a major end product	1448:1466	a major end product of fermentation	1448:1482	The strain SG0102T produced lactic acid as a major end product of fermentation.					
30796749	11	103	theme	major	1450:1454	arg1	acid					1440:1443	lactic acid	1433:1443	lactic acid	1433:1443	The strain SG0102T produced lactic acid as a major end product of fermentation.					
30796749	4	104	dep	NaCl	414:417	arg1	%					406:406	%	406:406	%	406:406	Furthermore, growth was observed in the presence of up to 3% (w/v) NaCl but not at salinity levels higher than 4%.					
30796749	7	105	theme	gene	1034:1037	arg1	sequence					1039:1046	16S rRNA gene sequence	1025:1046	16S rRNA gene sequence	1025:1046	The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102T belonged to the family Erysipelotrichaceae in the class Erysipelotrichia.					
30796749	14	106	theme	KCTC	1750:1753	arg1	SG0102T					1739:1745	SG0102T	1739:1745	SG0102T (= KCTC 15725T = NBRC 113396T)	1739:1776	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	14	106	theme	KCTC	1750:1753	arg1	113396T					1769:1775	= KCTC 15725T = NBRC 113396T	1748:1775	= KCTC 15725T = NBRC 113396T	1748:1775	The type strain is SG0102T (= KCTC 15725T = NBRC 113396T).					
30796749	8	107	theme	strain	1180:1185	arg1	SG0102T					1187:1193	the strain SG0102T	1176:1193	the strain SG0102T	1176:1193	The DNA G + C content of the strain SG0102T was 39.5 mol%.					
30796749	12	108	theme	phylogenetic	1515:1526	arg1	properties					1528:1537	These distinct phenotypic and phylogenetic properties	1485:1537	These distinct phenotypic and phylogenetic properties	1485:1537	These distinct phenotypic and phylogenetic properties suggest that strain SG0102T represents a novel species in a novel genus of the family Erysipelotrichaceae, for which the name Intestinibaculum porci gen. nov. sp.					
30796749	7	109	theme	16S	1025:1027	arg1	rRNA					1029:1032	16S rRNA	1025:1032	16S rRNA gene sequence	1025:1046	The phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SG0102T belonged to the family Erysipelotrichaceae in the class Erysipelotrichia.					
30796749	5	110	theme	Sharpea	666:672	arg1	%					702:702	91.0%	698:702	91.0%	698:702	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
30796749	5	110	theme	Sharpea	666:672	arg1	15217T					690:695	Sharpea azabuensis KCTC 15217T	666:695	Sharpea azabuensis KCTC 15217T (91.0%)	666:703	The comparative analysis of 16S rRNA gene sequences showed that strain SG0102T was most closely related to Kandleria vitulina DSM 20405T (93.3%), followed by Catenibacterium mitsuokai KCTC 5053T (91.1%), Sharpea azabuensis KCTC 15217T (91.0%), and Eggerthia catenaformis DSM 5348T (89.6%).					
31627307	0	0	from	Analysis	21:28	arg1	Hydrogels					77:85	3D Dextran Hydrogels	66:85	3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions	66:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	8	1	theme	hydrogel	1644:1651	arg1	system					1653:1658	the tailored hydrogel system	1631:1658	the tailored hydrogel system	1631:1658	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	5	2	theme	mal-dextran	917:927	arg1	hydrogel					929:936	mal-dextran hydrogel	917:936	mal-dextran hydrogel	917:936	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	4	3	theme	aggregations	879:890	arg1	law					846:848	the underlying regulation law	820:848	the underlying regulation law of C2C12 connections and 3T3 aggregations	820:890	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	8	4	theme	collective	1537:1546	arg1	behaviors					1557:1565	the collective cellular behaviors	1533:1565	the collective cellular behaviors	1533:1565	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	8	5	theme	RGD-composition	1452:1466	arg1	regulator					1520:1528	a regulator	1518:1528	a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials	1518:1695	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	8	5	theme	RGD-composition	1452:1466	arg1	hydrogel					1493:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	0	6	from	Characterization	0:15	arg1	Hydrogels					77:85	3D Dextran Hydrogels	66:85	3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions	66:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	0	7	with	Hydrogels	77:85	arg1	Compositions					121:132	Homogenous and Clustered RGD Compositions	92:132	Homogenous and Clustered RGD Compositions	92:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	4	8	theme	RGD	786:788	arg1	rates					801:805	four different RGD clustering rates	771:805	four different RGD clustering rates	771:805	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	5	9	theme	multicellular	1036:1048	arg1	structures					1050:1059	more self-organized multicellular structures	1016:1059	more self-organized multicellular structures relative to 2D culture	1016:1082	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	0	10	theme	Homogenous	92:101	arg1	Compositions					121:132	Homogenous and Clustered RGD Compositions	92:132	Homogenous and Clustered RGD Compositions	92:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	8	11	theme	tailored	1635:1642	arg1	system					1653:1658	the tailored hydrogel system	1631:1658	the tailored hydrogel system	1631:1658	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	4	12	theme	different	776:784	arg1	rates					801:805	four different RGD clustering rates	771:805	four different RGD clustering rates	771:805	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	4	13	theme	3D	745:746	arg1	hydrogels					756:764	3D dextran hydrogels	745:764	3D dextran hydrogels	745:764	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	0	14	theme	RGD	117:119	arg1	Compositions					121:132	Homogenous and Clustered RGD Compositions	92:132	Homogenous and Clustered RGD Compositions	92:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	0	15	from	Behaviors	53:61	arg1	Hydrogels					77:85	3D Dextran Hydrogels	66:85	3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions	66:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	4	16	theme	connections	859:869	arg1	law					846:848	the underlying regulation law	820:848	the underlying regulation law of C2C12 connections and 3T3 aggregations	820:890	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	4	17	theme	hydrogels	756:764	arg1	efficacy					733:740	the cell-adhesive efficacy	715:740	the cell-adhesive efficacy of 3D dextran hydrogels	715:764	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	8	18	theme	behaviors	1557:1565	arg1	regulator					1520:1528	a regulator	1518:1528	a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials	1518:1695	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	8	18	theme	behaviors	1557:1565	arg1	hydrogel					1493:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	6	19	theme	cell	1270:1273	arg1	degree					1287:1292	the 3T3 cell aggregating degree	1262:1292	the 3T3 cell aggregating degree	1262:1292	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	0	20	theme	Clustered	107:115	arg1	Compositions					121:132	Homogenous and Clustered RGD Compositions	92:132	Homogenous and Clustered RGD Compositions	92:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	4	21	theme	C2C12	853:857	arg1	connections					859:869	C2C12 connections	853:869	C2C12 connections	853:869	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	4	22	theme	clustering	790:799	arg1	rates					801:805	four different RGD clustering rates	771:805	four different RGD clustering rates	771:805	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	6	23	theme	RGD-clustered	1117:1129	arg1	hydrogel					1143:1150	RGD-clustered mal-dextran hydrogel	1117:1150	RGD-clustered mal-dextran hydrogel	1117:1150	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	3	24	theme	cells	679:683	arg1	behaviors					636:644	the evolutionary behaviors	619:644	the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells	619:683	Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells in 3D.					
31627307	6	25	theme	breadthwise-aggregated	1213:1234	arg1	connection					1236:1245	the breadthwise-aggregated connection	1209:1245	the breadthwise-aggregated connection	1209:1245	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	4	26	theme	3T3	875:877	arg1	aggregations					879:890	3T3 aggregations	875:890	3T3 aggregations	875:890	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	3	27	theme	degradable	565:574	arg1	crosslinkers					576:587	cell degradable crosslinkers	560:587	cell degradable crosslinkers	560:587	Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells in 3D.					
31627307	4	28	theme	dextran	748:754	arg1	hydrogels					756:764	3D dextran hydrogels	745:764	3D dextran hydrogels	745:764	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	6	29	theme	mal-dextran	1131:1141	arg1	hydrogel					1143:1150	RGD-clustered mal-dextran hydrogel	1117:1150	RGD-clustered mal-dextran hydrogel	1117:1150	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	2	30	theme	spatial	390:396	arg1	distribution					398:409	the spatial distribution	386:409	the spatial distribution of the adhesion sites for cells in 3D	386:447	However, the hydrogels that are usually used could not be parametrically regulated, especially for quantitatively regulating the spatial distribution of the adhesion sites for cells in 3D.					
31627307	8	31	theme	useful	1583:1588	arg1	information					1590:1600	useful information	1583:1600	useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials	1583:1695	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	6	32	theme	3T3	1266:1268	arg1	degree					1287:1292	the 3T3 cell aggregating degree	1262:1292	the 3T3 cell aggregating degree	1262:1292	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	5	33	theme	continuous	979:988	arg1	proliferation					990:1002	continuous proliferation	979:1002	continuous proliferation	979:1002	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	5	33	theme	continuous	979:988	arg1	cells					952:956	cells stable viability and continuous proliferation	952:1002	cells stable viability and continuous proliferation	952:1002	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	1	34	theme	important	206:214	arg1	role					216:219	an important role	203:219	an important role	203:219	The interactions between substrate materials and cells usually play an important role in the hydrogel-based 3D cell cultures.					
31627307	8	35	theme	Such	1447:1450	arg1	regulator					1520:1528	a regulator	1518:1528	a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials	1518:1695	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	8	35	theme	Such	1447:1450	arg1	hydrogel					1493:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	8	36	theme	mal-dextran	1481:1491	arg1	regulator					1520:1528	a regulator	1518:1528	a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials	1518:1695	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	8	36	theme	mal-dextran	1481:1491	arg1	hydrogel					1493:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	3	37	theme	C2C12	673:677	arg1	cells					679:683	C2C12 cells	673:683	C2C12 cells	673:683	Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells in 3D.					
31627307	3	38	theme	cell	560:563	arg1	crosslinkers					576:587	cell degradable crosslinkers	560:587	cell degradable crosslinkers	560:587	Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells in 3D.					
31627307	7	39	theme	hydrogel	1374:1381	arg1	advantages					1345:1354	the advantages	1341:1354	the advantages of RGD clustering hydrogel	1341:1381	Further, the advantages of RGD clustering hydrogel could be amplified by appropriately reducing RGD concentration.					
31627307	0	40	theme	Cellular	44:51	arg1	Behaviors					53:61	Collective Cellular Behaviors	33:61	Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions	33:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	6	41	theme	C2C12	1183:1187	arg1	elongation					1194:1203	C2C12 cell elongation	1183:1203	C2C12 cell elongation	1183:1203	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	0	42	from	Hydrogels	77:85	arg1	Analysis					21:28	Analysis	21:28	Analysis	21:28	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	0	42	from	Hydrogels	77:85	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	4	43	theme	regulation	835:844	arg1	law					846:848	the underlying regulation law	820:848	the underlying regulation law of C2C12 connections and 3T3 aggregations	820:890	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	2	44	theme	sites	427:431	arg1	distribution					398:409	the spatial distribution	386:409	the spatial distribution of the adhesion sites for cells in 3D	386:447	However, the hydrogels that are usually used could not be parametrically regulated, especially for quantitatively regulating the spatial distribution of the adhesion sites for cells in 3D.					
31627307	0	45	theme	Collective	33:42	arg1	Behaviors					53:61	Collective Cellular Behaviors	33:61	Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions	33:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	3	46	theme	fibroblasts	657:667	arg1	behaviors					636:644	the evolutionary behaviors	619:644	the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells	619:683	Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells in 3D.					
31627307	4	47	theme	underlying	824:833	arg1	law					846:848	the underlying regulation law	820:848	the underlying regulation law of C2C12 connections and 3T3 aggregations	820:890	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	2	48	theme	adhesion	418:425	arg1	sites					427:431	the adhesion sites	414:431	the adhesion sites for cells in 3D	414:447	However, the hydrogels that are usually used could not be parametrically regulated, especially for quantitatively regulating the spatial distribution of the adhesion sites for cells in 3D.					
31627307	5	49	dep	cells	952:956	arg1	viability					965:973	stable viability	958:973	stable viability	958:973	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	5	49	dep	cells	952:956	arg1	proliferation					990:1002	continuous proliferation	979:1002	continuous proliferation	979:1002	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	5	49	dep	cells	952:956	arg1	cells					952:956	cells stable viability and continuous proliferation	952:1002	cells stable viability and continuous proliferation	952:1002	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	8	50	theme	cellular	1548:1555	arg1	behaviors					1557:1565	the collective cellular behaviors	1533:1565	the collective cellular behaviors	1533:1565	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	6	51	theme	cell	1189:1192	arg1	elongation					1194:1203	C2C12 cell elongation	1183:1203	C2C12 cell elongation	1183:1203	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	0	52	theme	Behaviors	53:61	arg1	Analysis					21:28	Analysis	21:28	Analysis	21:28	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	0	52	theme	Behaviors	53:61	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	8	53	theme	advanced	1675:1682	arg1	biomaterials					1684:1695	advanced biomaterials	1675:1695	advanced biomaterials	1675:1695	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	4	54	theme	cell-adhesive	719:731	arg1	efficacy					733:740	the cell-adhesive efficacy	715:740	the cell-adhesive efficacy of 3D dextran hydrogels	715:764	Moreover, by comparing the cell-adhesive efficacy of 3D dextran hydrogels with four different RGD clustering rates, we explored the underlying regulation law of C2C12 connections and 3T3 aggregations.					
31627307	2	55	used	used	301:304	arg2	hydrogels					274:282	the hydrogels	270:282	the hydrogels that are usually used	270:304	However, the hydrogels that are usually used could not be parametrically regulated, especially for quantitatively regulating the spatial distribution of the adhesion sites for cells in 3D.					
31627307	6	56	contain	has	1152:1154	arg1	hydrogel					1143:1150	RGD-clustered mal-dextran hydrogel	1117:1150	RGD-clustered mal-dextran hydrogel	1117:1150	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	6	56	contain	has	1152:1154	arg2	advantage					1160:1168	the advantage	1156:1168	the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree	1156:1292	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	5	57	theme	self-organized	1021:1034	arg1	structures					1050:1059	more self-organized multicellular structures	1016:1059	more self-organized multicellular structures relative to 2D culture	1016:1082	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	5	58	theme	2D	1073:1074	arg1	culture					1076:1082	2D culture	1073:1082	2D culture	1073:1082	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	3	59	theme	semisynthetic	472:484	arg1	hydrogel					486:493	the semisynthetic hydrogel	468:493	the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers	468:587	Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells in 3D.					
31627307	8	60	theme	controllable	1468:1479	arg1	regulator					1520:1528	a regulator	1518:1528	a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials	1518:1695	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	8	60	theme	controllable	1468:1479	arg1	hydrogel					1493:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel	1447:1500	Such RGD-composition controllable mal-dextran hydrogel can function as a regulator of the collective cellular behaviors, which provides useful information for quantitatively designing the tailored hydrogel system and exploiting advanced biomaterials.					
31627307	1	61	theme	hydrogel-based	228:241	arg1	cultures					251:258	the hydrogel-based 3D cell cultures	224:258	the hydrogel-based 3D cell cultures	224:258	The interactions between substrate materials and cells usually play an important role in the hydrogel-based 3D cell cultures.					
31627307	6	62	theme	aggregating	1275:1285	arg1	degree					1287:1292	the 3T3 cell aggregating degree	1262:1292	the 3T3 cell aggregating degree	1262:1292	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31627307	3	63	theme	NIH-3T3	649:655	arg1	fibroblasts					657:667	NIH-3T3 fibroblasts	649:667	NIH-3T3 fibroblasts	649:667	Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells in 3D.					
31627307	5	64	theme	stable	958:963	arg1	viability					965:973	stable viability	958:973	stable viability	958:973	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	5	64	theme	stable	958:963	arg1	cells					952:956	cells stable viability and continuous proliferation	952:1002	cells stable viability and continuous proliferation	952:1002	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	1	65	theme	3D	243:244	arg1	cultures					251:258	the hydrogel-based 3D cell cultures	224:258	the hydrogel-based 3D cell cultures	224:258	The interactions between substrate materials and cells usually play an important role in the hydrogel-based 3D cell cultures.					
31627307	0	66	theme	Dextran	69:75	arg1	Hydrogels					77:85	3D Dextran Hydrogels	66:85	3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions	66:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	7	67	theme	RGD	1359:1361	arg1	hydrogel					1374:1381	RGD clustering hydrogel	1359:1381	RGD clustering hydrogel	1359:1381	Further, the advantages of RGD clustering hydrogel could be amplified by appropriately reducing RGD concentration.					
31627307	3	68	theme	evolutionary	623:634	arg1	behaviors					636:644	the evolutionary behaviors	619:644	the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells	619:683	Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells in 3D.					
31627307	7	69	theme	clustering	1363:1372	arg1	hydrogel					1374:1381	RGD clustering hydrogel	1359:1381	RGD clustering hydrogel	1359:1381	Further, the advantages of RGD clustering hydrogel could be amplified by appropriately reducing RGD concentration.					
31627307	3	70	theme	Arg-Gly-Asp	528:538	arg1	peptides					546:553	Arg-Gly-Asp (RGD) peptides	528:553	Arg-Gly-Asp (RGD) peptides	528:553	Here, we employed the semisynthetic hydrogel consisting of maleimide-dextran, Arg-Gly-Asp (RGD) peptides, and cell degradable crosslinkers to biochemically characterize the evolutionary behaviors of NIH-3T3 fibroblasts and C2C12 cells in 3D.					
31627307	1	71	theme	cell	246:249	arg1	cultures					251:258	the hydrogel-based 3D cell cultures	224:258	the hydrogel-based 3D cell cultures	224:258	The interactions between substrate materials and cells usually play an important role in the hydrogel-based 3D cell cultures.					
31627307	0	72	theme	3D	66:67	arg1	Hydrogels					77:85	3D Dextran Hydrogels	66:85	3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions	66:132	Characterization and Analysis of Collective Cellular Behaviors in 3D Dextran Hydrogels with Homogenous and Clustered RGD Compositions.					
31627307	7	73	theme	RGD	1428:1430	arg1	concentration					1432:1444	RGD concentration	1428:1444	RGD concentration	1428:1444	Further, the advantages of RGD clustering hydrogel could be amplified by appropriately reducing RGD concentration.					
31627307	5	74	theme	relative	1061:1068	arg1	structures					1050:1059	more self-organized multicellular structures	1016:1059	more self-organized multicellular structures relative to 2D culture	1016:1082	The results showed that mal-dextran hydrogel could promise cells stable viability and continuous proliferation, and induce more self-organized multicellular structures relative to 2D culture.					
31627307	2	75	from	cells	437:441	arg1	3D					446:447	3D	446:447	3D	446:447	However, the hydrogels that are usually used could not be parametrically regulated, especially for quantitatively regulating the spatial distribution of the adhesion sites for cells in 3D.					
31627307	1	76	theme	substrate	160:168	arg1	materials					170:178	substrate materials	160:178	substrate materials	160:178	The interactions between substrate materials and cells usually play an important role in the hydrogel-based 3D cell cultures.					
31627307	6	77	theme	homogenous	1316:1325	arg1	RGD					1327:1329	homogenous RGD	1316:1329	homogenous RGD	1316:1329	More importantly, we found that RGD-clustered mal-dextran hydrogel has the advantage of enhancing C2C12 cell elongation and the breadthwise-aggregated connection, and promoting the 3T3 cell aggregating degree compared to that with homogenous RGD.					
31786531	11	0	theme	bleeding	1713:1720	arg1	rates					1695:1699	similar rates	1687:1699	similar rates of MACE and bleeding	1687:1720	CONCLUSION The periprocedural use of bivalirudin vs UFH was associated with similar rates of MACE and bleeding.					
31786531	1	1	theme	routine	317:323	arg1	transfer					325:332	the routine transfer	313:332	the routine transfer to coronary angiography	313:356	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	12	2	theme	pharmacoinvasive	1864:1879	arg1	strategy					1881:1888	a pharmacoinvasive strategy	1862:1888	a pharmacoinvasive strategy	1862:1888	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	8	3	theme	Median	1213:1218	arg1	time					1244:1247	Median fibrinolysis to balloon time	1213:1247	Median fibrinolysis to balloon time	1213:1247	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	1	4	theme	infarction	245:254	arg1	management					264:273	ST-segment elevation myocardial infarction (STEMI) management	213:273	ST-segment elevation myocardial infarction (STEMI) management	213:273	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	12	5	from	anticoagulant	1845:1857	arg1	strategy					1881:1888	a pharmacoinvasive strategy	1862:1888	a pharmacoinvasive strategy	1862:1888	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	11	6	theme	bivalirudin	1648:1658	arg1	use					1641:1643	The periprocedural use	1622:1643	The periprocedural use of bivalirudin vs UFH	1622:1665	CONCLUSION The periprocedural use of bivalirudin vs UFH was associated with similar rates of MACE and bleeding.					
31786531	5	7	theme	primary	894:900	arg1	MACE					923:926	MACE	923:926	MACE	923:926	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	7	theme	primary	894:900	arg1	outcome					911:917	The primary efficacy outcome	890:917	The primary efficacy outcome	890:917	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	12	8	theme	periprocedural	1830:1843	arg1	anticoagulant					1845:1857	the first-line periprocedural anticoagulant	1815:1857	the first-line periprocedural anticoagulant in a pharmacoinvasive strategy	1815:1888	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	9	9	theme	UFH	1402:1404	arg1	group					1406:1410	the UFH group	1398:1410	the UFH group	1398:1410	Initial TIMI grade 3 flow was higher in the bivalirudin group vs the UFH group, but there was no difference in the rates post PCI.					
31786531	12	10	theme	superiority	1790:1800	arg1	lack					1760:1763	lack	1760:1763	lack of demonstrable clinical superiority	1760:1800	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	12	10	theme	superiority	1790:1800	arg1	expense					1733:1739	the expense	1729:1739	the expense of bivalirudin	1729:1754	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	11	11	theme	UFH	1663:1665	arg1	use					1641:1643	The periprocedural use	1622:1643	The periprocedural use of bivalirudin vs UFH	1622:1665	CONCLUSION The periprocedural use of bivalirudin vs UFH was associated with similar rates of MACE and bleeding.					
31786531	3	12	theme	unfractionated	627:640	arg1	heparin					642:648	unfractionated heparin	627:648	unfractionated heparin (UFH) used during PCI as part of a pharmacoinvasive strategy	627:709	We sought to assess the bivalirudin compared with unfractionated heparin (UFH) used during PCI as part of a pharmacoinvasive strategy.					
31786531	3	12	theme	unfractionated	627:640	arg1	UFH					651:653	UFH	651:653	UFH	651:653	We sought to assess the bivalirudin compared with unfractionated heparin (UFH) used during PCI as part of a pharmacoinvasive strategy.					
31786531	10	13	from	UFH	1592:1594	arg1	patients					1555:1562	patients	1555:1562	patients treated with bivalirudin	1555:1587	MACE rates were 4.9% vs 7.8% (P=.40) and TIMI bleeding rates were 7.3% vs 11.7% (P=.29) in patients treated with bivalirudin vs UFH, respectively.					
31786531	8	14	theme	bivalirudin	1272:1282	arg1	group					1284:1288	the bivalirudin group	1268:1288	the bivalirudin group	1268:1288	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	1	15	theme	pharmacoinvasive	183:198	arg1	strategy					200:207	A pharmacoinvasive strategy	181:207	A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management	181:273	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	4	16	dep	April	816:820	arg1	2011					835:838	2011	835:838	2011	835:838	METHODS We identified consecutive patients referred to the University of Ottawa Heart Institute between April 2009 and May 2011 as part of a pharmacoinvasive strategy for STEMI.					
31786531	6	17	theme	safety	1028:1033	arg1	outcome					1035:1041	The primary safety outcome	1016:1041	The primary safety outcome	1016:1041	The primary safety outcome was TIMI bleeding.					
31786531	6	17	theme	safety	1028:1033	arg1	bleeding					1052:1059	bleeding	1052:1059	bleeding	1052:1059	The primary safety outcome was TIMI bleeding.					
31786531	4	18	theme	consecutive	734:744	arg1	patients					746:753	consecutive patients	734:753	consecutive patients referred to the University of Ottawa Heart Institute between April 2009 and May 2011 as part of a pharmacoinvasive strategy for STEMI	734:887	METHODS We identified consecutive patients referred to the University of Ottawa Heart Institute between April 2009 and May 2011 as part of a pharmacoinvasive strategy for STEMI.					
31786531	1	19	theme	elevation	224:232	arg1	STEMI					257:261	STEMI	257:261	STEMI	257:261	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	1	19	theme	elevation	224:232	arg1	infarction					245:254	ST-segment elevation myocardial infarction	213:254	ST-segment elevation myocardial infarction (STEMI) management	213:273	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	0	20	theme	ST-Segment	126:135	arg1	Infarction					158:167	ST-Segment Elevation Myocardial Infarction	126:167	ST-Segment Elevation Myocardial Infarction	126:167	Periprocedural Bivalirudin Versus Unfractionated Heparin During Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction.					
31786531	5	21	theme	stroke	979:984	arg1	reinfarction					962:973	reinfarction	962:973	reinfarction	962:973	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	21	theme	stroke	979:984	arg1	stroke					979:984	stroke	979:984	stroke	979:984	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	21	theme	stroke	979:984	arg1	death					955:959	death	955:959	death	955:959	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	21	theme	stroke	979:984	arg1	composite					942:950	a composite	940:950	a composite of death, reinfarction, or stroke during index hospitalization	940:1013	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	2	22	theme	higher	555:560	arg1	risk					571:574	higher bleeding risk	555:574	higher bleeding risk	555:574	This method reduces the risk of major adverse cardiovascular event (MACE) compared with fibrinolysis alone; however, it is associated with higher bleeding risk.					
31786531	2	23	theme	major	448:452	arg1	MACE					484:487	MACE	484:487	MACE	484:487	This method reduces the risk of major adverse cardiovascular event (MACE) compared with fibrinolysis alone; however, it is associated with higher bleeding risk.					
31786531	2	23	theme	major	448:452	arg1	event					477:481	major adverse cardiovascular event	448:481	major adverse cardiovascular event (MACE)	448:488	This method reduces the risk of major adverse cardiovascular event (MACE) compared with fibrinolysis alone; however, it is associated with higher bleeding risk.					
31786531	0	24	theme	Myocardial	147:156	arg1	Infarction					158:167	ST-Segment Elevation Myocardial Infarction	126:167	ST-Segment Elevation Myocardial Infarction	126:167	Periprocedural Bivalirudin Versus Unfractionated Heparin During Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction.					
31786531	8	25	from	minutes	1298:1304	arg1	P<.001					1324:1329	P<.001	1324:1329	P<.001	1324:1329	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	8	25	from	minutes	1298:1304	arg1	group					1317:1321	the UFH group	1309:1321	the UFH group (P<.001)	1309:1330	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	8	25	from	minutes	1298:1304	arg1	group					1284:1288	the bivalirudin group	1268:1288	the bivalirudin group	1268:1288	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	5	26	theme	index	993:997	arg1	hospitalization					999:1013	index hospitalization	993:1013	index hospitalization	993:1013	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	7	27	from	patients	1178:1185	arg1	group					1165:1169	the bivalirudin group	1149:1169	the bivalirudin group	1149:1169	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	7	27	from	patients	1178:1185	arg1	group					1206:1210	the UFH group	1198:1210	the UFH group	1198:1210	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	0	28	theme	Periprocedural	0:13	arg1	Bivalirudin					15:25	Periprocedural Bivalirudin	0:25	Periprocedural Bivalirudin	0:25	Periprocedural Bivalirudin Versus Unfractionated Heparin During Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction.					
31786531	1	29	theme	coronary	337:344	arg1	angiography					346:356	coronary angiography	337:356	coronary angiography	337:356	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	4	30	theme	Heart	792:796	arg1	Institute					798:806	Ottawa Heart Institute	785:806	Ottawa Heart Institute between April 2009 and May 2011	785:838	METHODS We identified consecutive patients referred to the University of Ottawa Heart Institute between April 2009 and May 2011 as part of a pharmacoinvasive strategy for STEMI.					
31786531	2	31	theme	cardiovascular	462:475	arg1	MACE					484:487	MACE	484:487	MACE	484:487	This method reduces the risk of major adverse cardiovascular event (MACE) compared with fibrinolysis alone; however, it is associated with higher bleeding risk.					
31786531	2	31	theme	cardiovascular	462:475	arg1	event					477:481	major adverse cardiovascular event	448:481	major adverse cardiovascular event (MACE)	448:488	This method reduces the risk of major adverse cardiovascular event (MACE) compared with fibrinolysis alone; however, it is associated with higher bleeding risk.					
31786531	7	32	theme	bivalirudin	1153:1163	arg1	group					1165:1169	the bivalirudin group	1149:1169	the bivalirudin group	1149:1169	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	1	33	theme	percutaneous	364:375	arg1	PCI					400:402	PCI	400:402	PCI	400:402	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	1	33	theme	percutaneous	364:375	arg1	intervention					386:397	percutaneous coronary intervention	364:397	percutaneous coronary intervention (PCI)	364:403	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	4	34	dep	METHODS	712:718	arg1	identified					723:732	identified	723:732	identified consecutive patients referred to the University of Ottawa Heart Institute between April 2009 and May 2011 as part of a pharmacoinvasive strategy for STEMI	723:887	METHODS We identified consecutive patients referred to the University of Ottawa Heart Institute between April 2009 and May 2011 as part of a pharmacoinvasive strategy for STEMI.					
31786531	4	35	theme	pharmacoinvasive	853:868	arg1	strategy					870:877	a pharmacoinvasive strategy	851:877	a pharmacoinvasive strategy for STEMI	851:887	METHODS We identified consecutive patients referred to the University of Ottawa Heart Institute between April 2009 and May 2011 as part of a pharmacoinvasive strategy for STEMI.					
31786531	0	36	theme	Percutaneous	64:75	arg1	Intervention					86:97	Percutaneous Coronary Intervention	64:97	Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction	64:167	Periprocedural Bivalirudin Versus Unfractionated Heparin During Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction.					
31786531	11	37	theme	similar	1687:1693	arg1	rates					1695:1699	similar rates	1687:1699	similar rates of MACE and bleeding	1687:1720	CONCLUSION The periprocedural use of bivalirudin vs UFH was associated with similar rates of MACE and bleeding.					
31786531	9	38	theme	Initial	1333:1339	arg1	flow					1354:1357	Initial TIMI grade 3 flow	1333:1357	Initial TIMI grade 3 flow	1333:1357	Initial TIMI grade 3 flow was higher in the bivalirudin group vs the UFH group, but there was no difference in the rates post PCI.					
31786531	10	39	from	%	1483:1483	arg1	patients					1555:1562	patients	1555:1562	patients treated with bivalirudin	1555:1587	MACE rates were 4.9% vs 7.8% (P=.40) and TIMI bleeding rates were 7.3% vs 11.7% (P=.29) in patients treated with bivalirudin vs UFH, respectively.					
31786531	10	40	theme	MACE	1464:1467	arg1	rates					1469:1473	MACE rates	1464:1473	MACE rates	1464:1473	MACE rates were 4.9% vs 7.8% (P=.40) and TIMI bleeding rates were 7.3% vs 11.7% (P=.29) in patients treated with bivalirudin vs UFH, respectively.					
31786531	3	41	theme	pharmacoinvasive	685:700	arg1	strategy					702:709	a pharmacoinvasive strategy	683:709	a pharmacoinvasive strategy	683:709	We sought to assess the bivalirudin compared with unfractionated heparin (UFH) used during PCI as part of a pharmacoinvasive strategy.					
31786531	9	42	theme	bivalirudin	1377:1387	arg1	group					1389:1393	the bivalirudin group	1373:1393	the bivalirudin group	1373:1393	Initial TIMI grade 3 flow was higher in the bivalirudin group vs the UFH group, but there was no difference in the rates post PCI.					
31786531	9	43	theme	grade	1346:1350	arg1	flow					1354:1357	Initial TIMI grade 3 flow	1333:1357	Initial TIMI grade 3 flow	1333:1357	Initial TIMI grade 3 flow was higher in the bivalirudin group vs the UFH group, but there was no difference in the rates post PCI.					
31786531	10	44	from	%	1542:1542	arg1	patients					1555:1562	patients	1555:1562	patients treated with bivalirudin	1555:1587	MACE rates were 4.9% vs 7.8% (P=.40) and TIMI bleeding rates were 7.3% vs 11.7% (P=.29) in patients treated with bivalirudin vs UFH, respectively.					
31786531	7	45	dep	patients	1088:1095	arg1	%					1192:1192	37.5%	1188:1192	37.5%	1188:1192	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	7	45	dep	patients	1088:1095	arg1	%					1143:1143	61.5%	1139:1143	61.5%	1139:1143	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	7	45	dep	patients	1088:1095	arg1	patients					1129:1136	123 patients	1125:1136	123 patients (61.5%) in the bivalirudin group	1125:1169	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	7	45	dep	patients	1088:1095	arg1	patients					1178:1185	77 patients	1175:1185	77 patients (37.5%) in the UFH group	1175:1210	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	7	46	dep	RESULTS	1062:1068	arg1	identified					1073:1082	identified	1073:1082	identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group	1073:1210	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	1	47	theme	myocardial	234:243	arg1	STEMI					257:261	STEMI	257:261	STEMI	257:261	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	1	47	theme	myocardial	234:243	arg1	infarction					245:254	ST-segment elevation myocardial infarction	213:254	ST-segment elevation myocardial infarction (STEMI) management	213:273	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	4	48	theme	strategy	870:877	arg1	part					843:846	part	843:846	part of a pharmacoinvasive strategy for STEMI	843:887	METHODS We identified consecutive patients referred to the University of Ottawa Heart Institute between April 2009 and May 2011 as part of a pharmacoinvasive strategy for STEMI.					
31786531	7	49	theme	inclusion	1105:1113	arg1	criteria					1115:1122	inclusion criteria	1105:1122	inclusion criteria	1105:1122	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	5	50	theme	efficacy	902:909	arg1	MACE					923:926	MACE	923:926	MACE	923:926	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	50	theme	efficacy	902:909	arg1	outcome					911:917	The primary efficacy outcome	890:917	The primary efficacy outcome	890:917	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	8	51	theme	fibrinolysis	1220:1231	arg1	time					1244:1247	Median fibrinolysis to balloon time	1213:1247	Median fibrinolysis to balloon time	1213:1247	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	12	52	theme	first-line	1819:1828	arg1	anticoagulant					1845:1857	the first-line periprocedural anticoagulant	1815:1857	the first-line periprocedural anticoagulant in a pharmacoinvasive strategy	1815:1888	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	8	53	theme	balloon	1236:1242	arg1	time					1244:1247	Median fibrinolysis to balloon time	1213:1247	Median fibrinolysis to balloon time	1213:1247	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	10	54	theme	TIMI	1505:1508	arg1	rates					1519:1523	TIMI bleeding rates	1505:1523	TIMI bleeding rates	1505:1523	MACE rates were 4.9% vs 7.8% (P=.40) and TIMI bleeding rates were 7.3% vs 11.7% (P=.29) in patients treated with bivalirudin vs UFH, respectively.					
31786531	12	55	theme	clinical	1781:1788	arg1	superiority					1790:1800	demonstrable clinical superiority	1768:1800	demonstrable clinical superiority	1768:1800	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	11	56	dep	CONCLUSION	1611:1620	arg1	associated					1671:1680	associated	1671:1680	was associated with similar rates of MACE and bleeding	1667:1720	CONCLUSION The periprocedural use of bivalirudin vs UFH was associated with similar rates of MACE and bleeding.					
31786531	7	57	theme	UFH	1202:1204	arg1	group					1206:1210	the UFH group	1198:1210	the UFH group	1198:1210	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	1	58	theme	ST-segment	213:222	arg1	STEMI					257:261	STEMI	257:261	STEMI	257:261	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	1	58	theme	ST-segment	213:222	arg1	infarction					245:254	ST-segment elevation myocardial infarction	213:254	ST-segment elevation myocardial infarction (STEMI) management	213:273	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	6	59	theme	primary	1020:1026	arg1	outcome					1035:1041	The primary safety outcome	1016:1041	The primary safety outcome	1016:1041	The primary safety outcome was TIMI bleeding.					
31786531	6	59	theme	primary	1020:1026	arg1	bleeding					1052:1059	bleeding	1052:1059	bleeding	1052:1059	The primary safety outcome was TIMI bleeding.					
31786531	11	60	theme	periprocedural	1626:1639	arg1	use					1641:1643	The periprocedural use	1622:1643	The periprocedural use of bivalirudin vs UFH	1622:1665	CONCLUSION The periprocedural use of bivalirudin vs UFH was associated with similar rates of MACE and bleeding.					
31786531	0	61	theme	Elevation	137:145	arg1	Infarction					158:167	ST-Segment Elevation Myocardial Infarction	126:167	ST-Segment Elevation Myocardial Infarction	126:167	Periprocedural Bivalirudin Versus Unfractionated Heparin During Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction.					
31786531	12	62	theme	demonstrable	1768:1779	arg1	superiority					1790:1800	demonstrable clinical superiority	1768:1800	demonstrable clinical superiority	1768:1800	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	2	63	theme	bleeding	562:569	arg1	risk					571:574	higher bleeding risk	555:574	higher bleeding risk	555:574	This method reduces the risk of major adverse cardiovascular event (MACE) compared with fibrinolysis alone; however, it is associated with higher bleeding risk.					
31786531	8	64	from	minutes	1257:1263	arg1	P<.001					1324:1329	P<.001	1324:1329	P<.001	1324:1329	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	8	64	from	minutes	1257:1263	arg1	group					1317:1321	the UFH group	1309:1321	the UFH group (P<.001)	1309:1330	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	8	64	from	minutes	1257:1263	arg1	group					1284:1288	the bivalirudin group	1268:1288	the bivalirudin group	1268:1288	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	8	65	dep	time	1244:1247	arg1	to					1233:1234	to	1233:1234	to	1233:1234	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	1	66	dep	BACKGROUND	170:179	arg1	combines					275:282	combines	275:282	combines	275:282	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	12	67	theme	bivalirudin	1744:1754	arg1	lack					1760:1763	lack	1760:1763	lack of demonstrable clinical superiority	1760:1800	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	12	67	theme	bivalirudin	1744:1754	arg1	expense					1733:1739	the expense	1729:1739	the expense of bivalirudin	1729:1754	Given the expense of bivalirudin and lack of demonstrable clinical superiority, UFH remains the first-line periprocedural anticoagulant in a pharmacoinvasive strategy.					
31786531	5	68	theme	death	955:959	arg1	reinfarction					962:973	reinfarction	962:973	reinfarction	962:973	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	68	theme	death	955:959	arg1	stroke					979:984	stroke	979:984	stroke	979:984	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	68	theme	death	955:959	arg1	death					955:959	death	955:959	death	955:959	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	68	theme	death	955:959	arg1	composite					942:950	a composite	940:950	a composite of death, reinfarction, or stroke during index hospitalization	940:1013	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	10	69	theme	bleeding	1510:1517	arg1	rates					1519:1523	TIMI bleeding rates	1505:1523	TIMI bleeding rates	1505:1523	MACE rates were 4.9% vs 7.8% (P=.40) and TIMI bleeding rates were 7.3% vs 11.7% (P=.29) in patients treated with bivalirudin vs UFH, respectively.					
31786531	0	70	theme	Unfractionated	34:47	arg1	Heparin					49:55	Unfractionated Heparin	34:55	Unfractionated Heparin	34:55	Periprocedural Bivalirudin Versus Unfractionated Heparin During Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction.					
31786531	5	71	theme	reinfarction	962:973	arg1	reinfarction					962:973	reinfarction	962:973	reinfarction	962:973	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	71	theme	reinfarction	962:973	arg1	stroke					979:984	stroke	979:984	stroke	979:984	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	71	theme	reinfarction	962:973	arg1	death					955:959	death	955:959	death	955:959	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	5	71	theme	reinfarction	962:973	arg1	composite					942:950	a composite	940:950	a composite of death, reinfarction, or stroke during index hospitalization	940:1013	The primary efficacy outcome was MACE, defined as a composite of death, reinfarction, or stroke during index hospitalization.					
31786531	4	72	theme	Ottawa	785:790	arg1	Institute					798:806	Ottawa Heart Institute	785:806	Ottawa Heart Institute between April 2009 and May 2011	785:838	METHODS We identified consecutive patients referred to the University of Ottawa Heart Institute between April 2009 and May 2011 as part of a pharmacoinvasive strategy for STEMI.					
31786531	2	73	theme	event	477:481	arg1	risk					440:443	the risk	436:443	the risk of major adverse cardiovascular event (MACE)	436:488	This method reduces the risk of major adverse cardiovascular event (MACE) compared with fibrinolysis alone; however, it is associated with higher bleeding risk.					
31786531	7	74	from	patients	1129:1136	arg1	group					1165:1169	the bivalirudin group	1149:1169	the bivalirudin group	1149:1169	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	7	74	from	patients	1129:1136	arg1	group					1206:1210	the UFH group	1198:1210	the UFH group	1198:1210	RESULTS We identified 200 patients meeting inclusion criteria: 123 patients (61.5%) in the bivalirudin group and 77 patients (37.5%) in the UFH group.					
31786531	1	75	theme	fibrinolysis	295:306	arg1	use					288:290	the use	284:290	the use of fibrinolysis with the routine transfer to coronary angiography	284:356	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	2	76	theme	adverse	454:460	arg1	MACE					484:487	MACE	484:487	MACE	484:487	This method reduces the risk of major adverse cardiovascular event (MACE) compared with fibrinolysis alone; however, it is associated with higher bleeding risk.					
31786531	2	76	theme	adverse	454:460	arg1	event					477:481	major adverse cardiovascular event	448:481	major adverse cardiovascular event (MACE)	448:488	This method reduces the risk of major adverse cardiovascular event (MACE) compared with fibrinolysis alone; however, it is associated with higher bleeding risk.					
31786531	0	77	theme	Coronary	77:84	arg1	Intervention					86:97	Percutaneous Coronary Intervention	64:97	Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction	64:167	Periprocedural Bivalirudin Versus Unfractionated Heparin During Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction.					
31786531	1	78	theme	coronary	377:384	arg1	PCI					400:402	PCI	400:402	PCI	400:402	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	1	78	theme	coronary	377:384	arg1	intervention					386:397	percutaneous coronary intervention	364:397	percutaneous coronary intervention (PCI)	364:403	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	3	79	theme	strategy	702:709	arg1	part					675:678	part	675:678	part of a pharmacoinvasive strategy	675:709	We sought to assess the bivalirudin compared with unfractionated heparin (UFH) used during PCI as part of a pharmacoinvasive strategy.					
31786531	11	80	theme	MACE	1704:1707	arg1	rates					1695:1699	similar rates	1687:1699	similar rates of MACE and bleeding	1687:1720	CONCLUSION The periprocedural use of bivalirudin vs UFH was associated with similar rates of MACE and bleeding.					
31786531	1	81	with	use	288:290	arg1	transfer					325:332	the routine transfer	313:332	the routine transfer to coronary angiography	313:356	BACKGROUND A pharmacoinvasive strategy for ST-segment elevation myocardial infarction (STEMI) management combines the use of fibrinolysis with the routine transfer to coronary angiography, with percutaneous coronary intervention (PCI) if needed.					
31786531	8	82	theme	UFH	1313:1315	arg1	P<.001					1324:1329	P<.001	1324:1329	P<.001	1324:1329	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	8	82	theme	UFH	1313:1315	arg1	group					1317:1321	the UFH group	1309:1321	the UFH group (P<.001)	1309:1330	Median fibrinolysis to balloon time was 324 minutes in the bivalirudin group and 226 minutes in the UFH group (P<.001).					
31786531	9	83	theme	TIMI	1341:1344	arg1	flow					1354:1357	Initial TIMI grade 3 flow	1333:1357	Initial TIMI grade 3 flow	1333:1357	Initial TIMI grade 3 flow was higher in the bivalirudin group vs the UFH group, but there was no difference in the rates post PCI.					
31786531	0	84	dep	Bivalirudin	15:25	arg1	Intervention					86:97	Percutaneous Coronary Intervention	64:97	Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction	64:167	Periprocedural Bivalirudin Versus Unfractionated Heparin During Percutaneous Coronary Intervention Following Fibrinolysis for ST-Segment Elevation Myocardial Infarction.					
31786531	10	85	from	%	1533:1533	arg1	patients					1555:1562	patients	1555:1562	patients treated with bivalirudin	1555:1587	MACE rates were 4.9% vs 7.8% (P=.40) and TIMI bleeding rates were 7.3% vs 11.7% (P=.29) in patients treated with bivalirudin vs UFH, respectively.					
30715385	4	0	dep	METHODS	490:496	arg1	used					541:544	used	541:544	was used to test the factors diet, activity, and spermidine	537:595	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	5	1	dep	carbohydrate	830:841	arg1	fat					851:853	fat	851:853	carbohydrate:protein:fat	830:853	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	1	dep	carbohydrate	830:841	arg1	protein					843:849	protein	843:849	carbohydrate:protein:fat	830:853	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	9	2	theme	microbiota	1622:1631	arg1	P < 0.01					1646:1653	P < 0.01	1646:1653	P < 0.01	1646:1653	Activity influenced microbiota composition (P < 0.01) and reduced glucose concentrations in HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice compared with nonactive mice.					
30715385	9	2	theme	microbiota	1622:1631	arg1	composition					1633:1643	microbiota composition	1622:1643	microbiota composition (P < 0.01)	1622:1654	Activity influenced microbiota composition (P < 0.01) and reduced glucose concentrations in HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice compared with nonactive mice.					
30715385	12	3	theme	activity-induced	2148:2163	arg1	effects					2176:2182	activity-induced beneficial effects	2148:2182	activity-induced beneficial effects	2148:2182	Spermidine has the potential to augment activity-induced beneficial effects, particularly for sucrose-induced obesity.					
30715385	5	4	dep	HSD	825:827	arg1	%					858:858	70%	856:858	70%:20%:10% of energy; 35% sucrose	856:889	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	4	dep	HSD	825:827	arg1	carbohydrate					830:841	carbohydrate	830:841	carbohydrate:protein:fat	830:853	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	5	dep	diet	819:822	arg1	HSD					825:827	HSD	825:827	HSD	825:827	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	2	6	theme	activity-induced	297:312	arg1	loss					321:324	activity-induced weight loss	297:324	activity-induced weight loss	297:324	Polyamines such as spermidine are implicated in fat accumulation and may support activity-induced weight loss.					
30715385	1	7	theme	metabolic	196:204	arg1	syndrome					206:213	metabolic syndrome	196:213	metabolic syndrome	196:213	BACKGROUND Excess dietary fat and sugar are linked to obesity and metabolic syndrome.					
30715385	9	8	theme	reduced	1660:1666	arg1	concentrations					1676:1689	reduced glucose concentrations	1660:1689	reduced glucose concentrations	1660:1689	Activity influenced microbiota composition (P < 0.01) and reduced glucose concentrations in HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice compared with nonactive mice.					
30715385	3	9	theme	voluntary	401:409	arg1	activity					411:418	voluntary activity	401:418	voluntary activity	401:418	OBJECTIVE This study tested interventional spermidine supplementation and voluntary activity against fat- and sucrose-induced systemic and gut microbiota changes.					
30715385	11	10	from	CONCLUSIONS	1942:1952	arg1	mice					1971:1974	male C57BL/6N mice	1957:1974	male C57BL/6N mice	1957:1974	CONCLUSIONS In male C57BL/6N mice, dietary sucrose and fat caused diverse metabolic and microbiota changes that were differentially susceptible to physical exercise.					
30715385	12	11	theme	beneficial	2165:2174	arg1	effects					2176:2182	activity-induced beneficial effects	2148:2182	activity-induced beneficial effects	2148:2182	Spermidine has the potential to augment activity-induced beneficial effects, particularly for sucrose-induced obesity.					
30715385	4	12	theme	3-factorial	500:510	arg1	3 × 2 × 2					526:534	3 × 2 × 2	526:534	3 × 2 × 2	526:534	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	4	12	theme	3-factorial	500:510	arg1	design					518:523	A 3-factorial study design	498:523	A 3-factorial study design (3 × 2 × 2)	498:535	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	6	13	theme	3	1003:1003	arg1	mM					1005:1006	mM	1005:1006	mM	1005:1006	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	8	14	dep	P < 0.001	1504:1512	arg1	both					1499:1502	both	1499:1502	both	1499:1502	Microbiota taxonomic composition changed upon the HFD and HSD (both P < 0.001); however, only the HSD increased microbial diversity (P < 0.001) compared with the CD.					
30715385	9	15	theme	HSD-fed	1694:1700	arg1	P = 0.021					1703:1711	HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice	1694:1741	P = 0.021	1703:1711	Activity influenced microbiota composition (P < 0.01) and reduced glucose concentrations in HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice compared with nonactive mice.					
30715385	8	16	theme	Microbiota	1436:1445	arg1	composition					1457:1467	Microbiota taxonomic composition	1436:1467	Microbiota taxonomic composition	1436:1467	Microbiota taxonomic composition changed upon the HFD and HSD (both P < 0.001); however, only the HSD increased microbial diversity (P < 0.001) compared with the CD.					
30715385	1	17	theme	Excess	141:146	arg1	fat					156:158	BACKGROUND Excess dietary fat	130:158	BACKGROUND Excess dietary fat	130:158	BACKGROUND Excess dietary fat and sugar are linked to obesity and metabolic syndrome.					
30715385	10	18	theme	HSD+A+S	1891:1897	arg1	mice					1899:1902	HSD+A+S mice	1891:1902	HSD+A+S mice	1891:1902	The combination of activity and spermidine affected energy intake (P-interaction = 0.037) and reduced body weights of HSD+A+S mice compared with HSD+0 mice (P = 0.024).					
30715385	5	19	dep	%	683:683	arg1	%					691:691	10%	689:691	70%:20%:10% of energy; 7% sucrose	681:713	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	19	dep	%	683:683	arg1	sucrose					707:713	7% sucrose	704:713	70%:20%:10% of energy; 7% sucrose	681:713	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	19	dep	%	683:683	arg1	energy					696:701	energy	696:701	energy	696:701	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	19	dep	%	683:683	arg1	%					687:687	20%	685:687	70%:20%:10% of energy; 7% sucrose	681:713	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	20	theme	%	705:705	arg1	sucrose					707:713	7% sucrose	704:713	70%:20%:10% of energy; 7% sucrose	681:713	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	4	21	dep	factors	558:564	arg1	diet					566:569	diet	566:569	diet	566:569	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	4	21	dep	factors	558:564	arg1	factors					558:564	the factors diet, activity, and spermidine	554:595	the factors diet, activity, and spermidine	554:595	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	4	21	dep	factors	558:564	arg1	activity					572:579	activity	572:579	activity	572:579	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	4	21	dep	factors	558:564	arg1	spermidine					586:595	spermidine	586:595	spermidine	586:595	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	8	22	dep	HFD	1486:1488	arg1	the					1482:1484	the	1482:1484	the	1482:1484	Microbiota taxonomic composition changed upon the HFD and HSD (both P < 0.001); however, only the HSD increased microbial diversity (P < 0.001) compared with the CD.					
30715385	0	23	theme	Systemic	99:106	arg1	Changes					108:114	Fat-Induced Systemic Changes	87:114	Fat-Induced Systemic Changes in Male Mice	87:127	Spermidine and Voluntary Activity Exert Differential Effects on Sucrose- Compared with Fat-Induced Systemic Changes in Male Mice.					
30715385	3	24	theme	interventional	355:368	arg1	supplementation					381:395	interventional spermidine supplementation	355:395	interventional spermidine supplementation	355:395	OBJECTIVE This study tested interventional spermidine supplementation and voluntary activity against fat- and sucrose-induced systemic and gut microbiota changes.					
30715385	7	25	theme	plasma	1421:1426	arg1	lipids					1428:1433	plasma lipids	1421:1433	plasma lipids	1421:1433	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	11	26	theme	C57BL/6N	1962:1969	arg1	mice					1971:1974	male C57BL/6N mice	1957:1974	male C57BL/6N mice	1957:1974	CONCLUSIONS In male C57BL/6N mice, dietary sucrose and fat caused diverse metabolic and microbiota changes that were differentially susceptible to physical exercise.					
30715385	5	27	theme	energy	696:701	arg1	%					691:691	10%	689:691	70%:20%:10% of energy; 7% sucrose	681:713	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	27	theme	energy	696:701	arg1	energy					696:701	energy	696:701	energy	696:701	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	3	28	theme	gut	466:468	arg1	microbiota					470:479	gut microbiota	466:479	gut microbiota	466:479	OBJECTIVE This study tested interventional spermidine supplementation and voluntary activity against fat- and sucrose-induced systemic and gut microbiota changes.					
30715385	5	29	theme	6-wk-old	603:610	arg1	mice					621:624	Male 6-wk-old C57BL/6N mice	598:624	Male 6-wk-old C57BL/6N mice	598:624	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	8	30	theme	microbial	1548:1556	arg1	P < 0.001					1569:1577	P < 0.001	1569:1577	P < 0.001	1569:1577	Microbiota taxonomic composition changed upon the HFD and HSD (both P < 0.001); however, only the HSD increased microbial diversity (P < 0.001) compared with the CD.					
30715385	8	30	theme	microbial	1548:1556	arg1	diversity					1558:1566	microbial diversity	1548:1566	microbial diversity (P < 0.001)	1548:1578	Microbiota taxonomic composition changed upon the HFD and HSD (both P < 0.001); however, only the HSD increased microbial diversity (P < 0.001) compared with the CD.					
30715385	10	31	theme	mice	1899:1902	arg1	weights					1880:1886	body weights	1875:1886	body weights of HSD+A+S mice	1875:1902	The combination of activity and spermidine affected energy intake (P-interaction = 0.037) and reduced body weights of HSD+A+S mice compared with HSD+0 mice (P = 0.024).					
30715385	6	32	contain	had	933:935	arg2	access					947:952	unlimited access	937:952	unlimited access to running wheels	937:970	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	6	32	contain	had	933:935	arg1	groups					898:903	Diet groups	893:903	Diet groups	893:903	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	3	33	dep	OBJECTIVE	327:335	arg1	tested					348:353	tested	348:353	tested interventional spermidine supplementation and voluntary activity against fat- and sucrose-induced systemic and gut microbiota changes	348:487	OBJECTIVE This study tested interventional spermidine supplementation and voluntary activity against fat- and sucrose-induced systemic and gut microbiota changes.					
30715385	5	34	dep	%	767:767	arg1	energy					780:785	energy	780:785	energy	780:785	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	34	dep	%	767:767	arg1	%					771:771	20%	769:771	20%:20%:60% of energy	765:785	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	34	dep	%	767:767	arg1	%					775:775	60%	773:775	20%:20%:60% of energy	765:785	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	9	35	theme	nonactive	1757:1765	arg1	mice					1767:1770	nonactive mice	1757:1770	nonactive mice	1757:1770	Activity influenced microbiota composition (P < 0.01) and reduced glucose concentrations in HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice compared with nonactive mice.					
30715385	4	36	used	used	541:544	arg2	design					518:523	A 3-factorial study design	498:523	A 3-factorial study design (3 × 2 × 2)	498:535	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	4	36	used	used	541:544	arg2	3 × 2 × 2					526:534	3 × 2 × 2	526:534	3 × 2 × 2	526:534	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	5	37	theme	%	789:789	arg1	sucrose					791:797	7% sucrose	788:797	carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose	739:797	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	0	38	theme	Voluntary	15:23	arg1	Activity					25:32	Voluntary Activity	15:32	Voluntary Activity	15:32	Spermidine and Voluntary Activity Exert Differential Effects on Sucrose- Compared with Fat-Induced Systemic Changes in Male Mice.					
30715385	5	39	theme	%	881:881	arg1	sucrose					883:889	35% sucrose	879:889	70%:20%:10% of energy; 35% sucrose	856:889	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	7	40	theme	body	1147:1150	arg1	weights					1152:1158	body weights	1147:1158	body weights (by 36%, P < 0.001)	1147:1178	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	7	40	theme	body	1147:1150	arg1	P < 0.001					1169:1177	P < 0.001	1169:1177	P < 0.001	1169:1177	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	5	41	theme	high-sucrose	806:817	arg1	diet					819:822	a high-sucrose diet	804:822	a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose)	804:890	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	42	dep	%	858:858	arg1	energy					871:876	energy	871:876	energy	871:876	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	42	dep	%	858:858	arg1	%					862:862	20%	860:862	70%:20%:10% of energy; 35% sucrose	856:889	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	42	dep	%	858:858	arg1	%					866:866	10%	864:866	70%:20%:10% of energy; 35% sucrose	856:889	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	42	dep	%	858:858	arg1	sucrose					883:889	35% sucrose	879:889	70%:20%:10% of energy; 35% sucrose	856:889	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	43	dep	HFD	734:736	arg1	carbohydrate					739:750	carbohydrate	739:750	carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose	739:797	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	11	44	theme	physical	2089:2096	arg1	exercise					2098:2105	physical exercise	2089:2105	physical exercise	2089:2105	CONCLUSIONS In male C57BL/6N mice, dietary sucrose and fat caused diverse metabolic and microbiota changes that were differentially susceptible to physical exercise.					
30715385	5	45	dep	diet	728:731	arg1	HFD					734:736	HFD	734:736	HFD	734:736	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	46	theme	control	637:643	arg1	diet					645:648	a control diet	635:648	a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose)	635:714	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	10	47	theme	HSD+0	1918:1922	arg1	mice					1924:1927	HSD+0 mice	1918:1927	HSD+0 mice (P = 0.024)	1918:1939	The combination of activity and spermidine affected energy intake (P-interaction = 0.037) and reduced body weights of HSD+A+S mice compared with HSD+0 mice (P = 0.024).					
30715385	10	47	theme	HSD+0	1918:1922	arg1	P = 0.024					1930:1938	P = 0.024	1930:1938	P = 0.024	1930:1938	The combination of activity and spermidine affected energy intake (P-interaction = 0.037) and reduced body weights of HSD+A+S mice compared with HSD+0 mice (P = 0.024).					
30715385	6	48	theme	Diet	893:896	arg1	groups					898:903	Diet groups	893:903	Diet groups	893:903	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	6	49	theme	running	957:963	arg1	wheels					965:970	running wheels	957:970	running wheels	957:970	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	2	50	theme	weight	314:319	arg1	loss					321:324	activity-induced weight loss	297:324	activity-induced weight loss	297:324	Polyamines such as spermidine are implicated in fat accumulation and may support activity-induced weight loss.					
30715385	10	51	theme	activity	1792:1799	arg1	combination					1777:1787	The combination	1773:1787	The combination of activity and spermidine	1773:1814	The combination of activity and spermidine affected energy intake (P-interaction = 0.037) and reduced body weights of HSD+A+S mice compared with HSD+0 mice (P = 0.024).					
30715385	12	52	theme	sucrose-induced	2202:2216	arg1	obesity					2218:2224	sucrose-induced obesity	2202:2224	sucrose-induced obesity	2202:2224	Spermidine has the potential to augment activity-induced beneficial effects, particularly for sucrose-induced obesity.					
30715385	11	53	theme	dietary	1977:1983	arg1	CONCLUSIONS					1942:1952	CONCLUSIONS	1942:1952	CONCLUSIONS In male C57BL/6N mice	1942:1974	CONCLUSIONS In male C57BL/6N mice, dietary sucrose and fat caused diverse metabolic and microbiota changes that were differentially susceptible to physical exercise.					
30715385	11	53	theme	dietary	1977:1983	arg1	sucrose					1985:1991	dietary sucrose	1977:1991	dietary sucrose	1977:1991	CONCLUSIONS In male C57BL/6N mice, dietary sucrose and fat caused diverse metabolic and microbiota changes that were differentially susceptible to physical exercise.					
30715385	6	54	theme	unlimited	937:945	arg1	access					947:952	unlimited access	937:952	unlimited access to running wheels	937:970	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	9	55	theme	glucose	1668:1674	arg1	concentrations					1676:1689	reduced glucose concentrations	1660:1689	reduced glucose concentrations	1660:1689	Activity influenced microbiota composition (P < 0.01) and reduced glucose concentrations in HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice compared with nonactive mice.					
30715385	6	56	theme	mM	1005:1006	arg1	spermidine					1008:1017	3 mM spermidine	1003:1017	3 mM spermidine	1003:1017	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	10	57	theme	energy	1825:1830	arg1	P-interaction = 0.037					1840:1860	P-interaction = 0.037	1840:1860	P-interaction = 0.037	1840:1860	The combination of activity and spermidine affected energy intake (P-interaction = 0.037) and reduced body weights of HSD+A+S mice compared with HSD+0 mice (P = 0.024).					
30715385	10	57	theme	energy	1825:1830	arg1	intake					1832:1837	energy intake	1825:1837	energy intake (P-interaction = 0.037)	1825:1861	The combination of activity and spermidine affected energy intake (P-interaction = 0.037) and reduced body weights of HSD+A+S mice compared with HSD+0 mice (P = 0.024).					
30715385	6	58	dep	untreated	915:923	arg1	+0					926:927	+0	926:927	+0	926:927	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	6	58	dep	untreated	915:923	arg1	+A					973:974	+A	973:974	+A	973:974	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	8	59	theme	taxonomic	1447:1455	arg1	composition					1457:1467	Microbiota taxonomic composition	1436:1467	Microbiota taxonomic composition	1436:1467	Microbiota taxonomic composition changed upon the HFD and HSD (both P < 0.001); however, only the HSD increased microbial diversity (P < 0.001) compared with the CD.					
30715385	2	60	theme	fat	264:266	arg1	accumulation					268:279	fat accumulation	264:279	fat accumulation	264:279	Polyamines such as spermidine are implicated in fat accumulation and may support activity-induced weight loss.					
30715385	1	61	theme	BACKGROUND	130:139	arg1	fat					156:158	BACKGROUND Excess dietary fat	130:158	BACKGROUND Excess dietary fat	130:158	BACKGROUND Excess dietary fat and sugar are linked to obesity and metabolic syndrome.					
30715385	7	62	theme	plasma	1181:1186	arg1	cholesterol					1196:1206	cholesterol	1196:1206	cholesterol by 24%	1196:1213	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	7	62	theme	plasma	1181:1186	arg1	lipids					1188:1193	plasma lipids	1181:1193	plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004)	1181:1258	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	10	63	theme	spermidine	1805:1814	arg1	combination					1777:1787	The combination	1773:1787	The combination of activity and spermidine	1773:1814	The combination of activity and spermidine affected energy intake (P-interaction = 0.037) and reduced body weights of HSD+A+S mice compared with HSD+0 mice (P = 0.024).					
30715385	0	64	from	Changes	108:114	arg1	Mice					124:127	Male Mice	119:127	Male Mice	119:127	Spermidine and Voluntary Activity Exert Differential Effects on Sucrose- Compared with Fat-Induced Systemic Changes in Male Mice.					
30715385	6	65	theme	both	1063:1066	arg1	combination					1048:1058	a combination	1046:1058	a combination of both (+A+S)	1046:1073	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	6	65	theme	both	1063:1066	arg1	+S					1039:1040	+S	1039:1040	+S	1039:1040	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	6	65	theme	both	1063:1066	arg1	water					1032:1036	water	1032:1036	water (+S)	1032:1041	Diet groups were left untreated (+0) or had unlimited access to running wheels (+A) or were supplemented with 3 mM spermidine via drinking water (+S) or a combination of both (+A+S) for 30 wk (n = 7-10).					
30715385	1	66	theme	dietary	148:154	arg1	fat					156:158	BACKGROUND Excess dietary fat	130:158	BACKGROUND Excess dietary fat	130:158	BACKGROUND Excess dietary fat and sugar are linked to obesity and metabolic syndrome.					
30715385	12	67	contain	has	2119:2121	arg1	Spermidine					2108:2117	Spermidine	2108:2117	Spermidine	2108:2117	Spermidine has the potential to augment activity-induced beneficial effects, particularly for sucrose-induced obesity.					
30715385	12	67	contain	has	2119:2121	arg2	potential					2127:2135	the potential to augment activity-induced beneficial effects	2123:2182	the potential to augment activity-induced beneficial effects	2123:2182	Spermidine has the potential to augment activity-induced beneficial effects, particularly for sucrose-induced obesity.					
30715385	0	68	theme	Fat-Induced	87:97	arg1	Changes					108:114	Fat-Induced Systemic Changes	87:114	Fat-Induced Systemic Changes in Male Mice	87:127	Spermidine and Voluntary Activity Exert Differential Effects on Sucrose- Compared with Fat-Induced Systemic Changes in Male Mice.					
30715385	5	69	theme	energy	871:876	arg1	energy					871:876	energy	871:876	energy	871:876	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	69	theme	energy	871:876	arg1	%					866:866	10%	864:866	70%:20%:10% of energy; 35% sucrose	856:889	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	9	70	theme	HFD-fed	1718:1724	arg1	mice					1738:1741	HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice	1694:1741	mice	1738:1741	Activity influenced microbiota composition (P < 0.01) and reduced glucose concentrations in HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice compared with nonactive mice.					
30715385	7	71	theme	glucose	1265:1271	arg1	concentrations					1273:1286	glucose concentrations	1265:1286	glucose concentrations (by 18%, P < 0.001)	1265:1306	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	7	71	theme	glucose	1265:1271	arg1	P < 0.001					1297:1305	P < 0.001	1297:1305	P < 0.001	1297:1305	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	3	72	dep	fat-	428:431	arg1	changes					481:487	changes	481:487	changes	481:487	OBJECTIVE This study tested interventional spermidine supplementation and voluntary activity against fat- and sucrose-induced systemic and gut microbiota changes.					
30715385	3	72	dep	fat-	428:431	arg1	systemic					453:460	systemic	453:460	systemic	453:460	OBJECTIVE This study tested interventional spermidine supplementation and voluntary activity against fat- and sucrose-induced systemic and gut microbiota changes.					
30715385	9	73	theme	P < 0.001	1727:1735	arg1	mice					1738:1741	HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice	1694:1741	mice	1738:1741	Activity influenced microbiota composition (P < 0.01) and reduced glucose concentrations in HSD-fed (P = 0.021) and HFD-fed (P < 0.001) mice compared with nonactive mice.					
30715385	0	74	theme	Male	119:122	arg1	Mice					124:127	Male Mice	119:127	Male Mice	119:127	Spermidine and Voluntary Activity Exert Differential Effects on Sucrose- Compared with Fat-Induced Systemic Changes in Male Mice.					
30715385	7	75	dep	cholesterol	1196:1206	arg1	P = 0.004					1249:1257	P = 0.004	1249:1257	P = 0.004	1249:1257	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	7	75	dep	cholesterol	1196:1206	arg1	triglycerides					1227:1239	triglycerides	1227:1239	triglycerides by 27%	1227:1246	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	7	75	dep	cholesterol	1196:1206	arg1	P < 0.001					1216:1224	P < 0.001	1216:1224	P < 0.001	1216:1224	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	3	76	theme	spermidine	370:379	arg1	supplementation					381:395	interventional spermidine supplementation	355:395	interventional spermidine supplementation	355:395	OBJECTIVE This study tested interventional spermidine supplementation and voluntary activity against fat- and sucrose-induced systemic and gut microbiota changes.					
30715385	11	77	theme	male	1957:1960	arg1	mice					1971:1974	male C57BL/6N mice	1957:1974	male C57BL/6N mice	1957:1974	CONCLUSIONS In male C57BL/6N mice, dietary sucrose and fat caused diverse metabolic and microbiota changes that were differentially susceptible to physical exercise.					
30715385	10	78	theme	body	1875:1878	arg1	weights					1880:1886	body weights	1875:1886	body weights of HSD+A+S mice	1875:1902	The combination of activity and spermidine affected energy intake (P-interaction = 0.037) and reduced body weights of HSD+A+S mice compared with HSD+0 mice (P = 0.024).					
30715385	5	79	theme	high-fat	719:726	arg1	diet					728:731	a high-fat diet	717:731	a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose)	717:798	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	80	theme	Male	598:601	arg1	mice					621:624	Male 6-wk-old C57BL/6N mice	598:624	Male 6-wk-old C57BL/6N mice	598:624	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	81	theme	energy	780:785	arg1	energy					780:785	energy	780:785	energy	780:785	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	81	theme	energy	780:785	arg1	%					775:775	60%	773:775	20%:20%:60% of energy	765:785	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	82	theme	C57BL/6N	612:619	arg1	mice					621:624	Male 6-wk-old C57BL/6N mice	598:624	Male 6-wk-old C57BL/6N mice	598:624	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	83	dep	carbohydrate	655:666	arg1	fat					676:678	fat	676:678	carbohydrate:protein:fat	655:678	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	83	dep	carbohydrate	655:666	arg1	protein					668:674	protein	668:674	carbohydrate:protein:fat	655:678	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	1	84	attach	linked	174:179	arg2	sugar					164:168	sugar	164:168	sugar	164:168	BACKGROUND Excess dietary fat and sugar are linked to obesity and metabolic syndrome.					
30715385	1	84	attach	linked	174:179	arg2	fat					156:158	BACKGROUND Excess dietary fat	130:158	BACKGROUND Excess dietary fat	130:158	BACKGROUND Excess dietary fat and sugar are linked to obesity and metabolic syndrome.					
30715385	1	84	attach	linked	174:179	arg1	obesity					184:190	obesity	184:190	obesity	184:190	BACKGROUND Excess dietary fat and sugar are linked to obesity and metabolic syndrome.					
30715385	1	84	attach	linked	174:179	arg1	syndrome					206:213	metabolic syndrome	196:213	metabolic syndrome	196:213	BACKGROUND Excess dietary fat and sugar are linked to obesity and metabolic syndrome.					
30715385	0	85	theme	Differential	40:51	arg1	Effects					53:59	Differential Effects	40:59	Differential Effects	40:59	Spermidine and Voluntary Activity Exert Differential Effects on Sucrose- Compared with Fat-Induced Systemic Changes in Male Mice.					
30715385	5	86	dep	CD	651:652	arg1	carbohydrate					655:666	carbohydrate	655:666	carbohydrate:protein:fat	655:678	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	86	dep	CD	651:652	arg1	%					683:683	70%	681:683	70%:20%:10% of energy; 7% sucrose	681:713	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	7	87	theme	fasting	1365:1371	arg1	glucose					1373:1379	fasting glucose	1365:1379	fasting glucose	1365:1379	RESULTS In comparison to the CD, the HFD enhanced body weights (by 36%, P < 0.001), plasma lipids (cholesterol by 24%, P < 0.001; triglycerides by 27%, P = 0.004), and glucose concentrations (by 18%, P < 0.001), whereas the HSD increased weight by 13% (P < 0.001) and fasting glucose by 17% (P < 0.001) but did not increase plasma lipids.					
30715385	5	88	dep	diet	645:648	arg1	CD					651:652	CD	651:652	CD	651:652	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	4	89	theme	study	512:516	arg1	3 × 2 × 2					526:534	3 × 2 × 2	526:534	3 × 2 × 2	526:534	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	4	89	theme	study	512:516	arg1	design					518:523	A 3-factorial study design	498:523	A 3-factorial study design (3 × 2 × 2)	498:535	METHODS A 3-factorial study design (3 × 2 × 2) was used to test the factors diet, activity, and spermidine.					
30715385	11	90	theme	microbiota	2030:2039	arg1	changes					2041:2047	diverse metabolic and microbiota changes	2008:2047	changes	2041:2047	CONCLUSIONS In male C57BL/6N mice, dietary sucrose and fat caused diverse metabolic and microbiota changes that were differentially susceptible to physical exercise.					
30715385	5	91	dep	carbohydrate	739:750	arg1	fat					760:762	fat	760:762	fat	760:762	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	91	dep	carbohydrate	739:750	arg1	%					767:767	20%	765:767	20%:20%:60% of energy	765:785	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	91	dep	carbohydrate	739:750	arg1	protein					752:758	protein	752:758	carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose	739:797	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
30715385	5	91	dep	carbohydrate	739:750	arg1	sucrose					791:797	7% sucrose	788:797	carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose	739:797	Male 6-wk-old C57BL/6N mice were fed a control diet (CD; carbohydrate:protein:fat, 70%:20%:10% of energy; 7% sucrose), a high-fat diet (HFD; carbohydrate:protein:fat, 20%:20%:60% of energy; 7% sucrose), or a high-sucrose diet (HSD; carbohydrate:protein:fat, 70%:20%:10% of energy; 35% sucrose).					
31036501	13	0	from	role	1655:1658	arg1	photoaging					1692:1701	photoaging	1692:1701	photoaging	1692:1701	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	0	from	role	1655:1658	arg1	models					1713:1718	animal models	1706:1718	animal models	1706:1718	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	0	from	role	1655:1658	arg1	skin					1741:1744	skin	1741:1744	skin	1741:1744	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	3	1	from	expression	447:456	arg1	mice					500:503	mice	500:503	mice	500:503	Herein, we systematically compared the protein-level increase and gene expression between HAc-micro-HAp and HAc-nano-HAp in mice and determined the mechanisms underlying the biological responses to HAc and HAp.					
31036501	2	2	theme	HAp	362:364	arg1	fillers					367:373	composite HAc-hydroxyapatite (HAp) fillers	332:373	composite HAc-hydroxyapatite (HAp) fillers	332:373	We previously developed composite HAc-hydroxyapatite (HAp) fillers.					
31036501	7	3	theme	blot	904:907	arg1	analysis					909:916	Western blot analysis	896:916	Western blot analysis	896:916	Western blot analysis, real-time polymerase chain reaction analysis, and immunohistochemistry were performed to investigate protein and gene expression changes.					
31036501	4	4	theme	BALB/c-nude	608:618	arg1	mice					620:623	Five-week-old female BALB/c-nude mice	587:623	Five-week-old female BALB/c-nude mice	587:623	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	1	5	link	cross-linked	204:215	arg1	HAc					234:236	HAc	234:236	HAc	234:236	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	1	5	link	cross-linked	204:215	arg1	acid					228:231	cross-linked hyaluronic acid	204:231	cross-linked hyaluronic acid (HAc)	204:237	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	9	6	theme	pure	1254:1257	arg1	fillers					1259:1265	pure fillers	1254:1265	pure fillers	1254:1265	Protein and gene expression analyses indicated that, compared with pure fillers, HAc-nano-HAp and HAc-micro-HAp hydrogels preferentially promoted collagen and elastic fiber formation through the TGF-β pathway.					
31036501	8	7	theme	Organ	1057:1061	arg1	toxicity					1097:1104	Organ (liver, lung, spleen, and kidney) toxicity	1057:1104	Organ (liver, lung, spleen, and kidney) toxicity of HAc-nano-HAp	1057:1120	Organ (liver, lung, spleen, and kidney) toxicity of HAc-nano-HAp was determined by hematoxylin and eosin staining after 12 weeks.					
31036501	4	8	theme	female	601:606	arg1	mice					620:623	Five-week-old female BALB/c-nude mice	587:623	Five-week-old female BALB/c-nude mice	587:623	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	9	9	theme	Protein	1187:1193	arg1	analyses					1215:1222	Protein and gene expression analyses	1187:1222	Protein and gene expression analyses	1187:1222	Protein and gene expression analyses indicated that, compared with pure fillers, HAc-nano-HAp and HAc-micro-HAp hydrogels preferentially promoted collagen and elastic fiber formation through the TGF-β pathway.					
31036501	1	10	theme	superior	248:255	arg1	filler					300:305	a dermal filler	291:305	a dermal filler	291:305	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	1	10	theme	superior	248:255	arg1	longevity					278:286	longevity	278:286	longevity	278:286	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	1	10	theme	superior	248:255	arg1	biocompatibility					257:272	superior biocompatibility	248:272	superior biocompatibility	248:272	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	7	11	theme	protein	1020:1026	arg1	changes					1048:1054	protein and gene expression changes	1020:1054	changes	1048:1054	Western blot analysis, real-time polymerase chain reaction analysis, and immunohistochemistry were performed to investigate protein and gene expression changes.					
31036501	9	12	theme	collagen	1333:1340	arg1	formation					1360:1368	collagen and elastic fiber formation	1333:1368	formation	1360:1368	Protein and gene expression analyses indicated that, compared with pure fillers, HAc-nano-HAp and HAc-micro-HAp hydrogels preferentially promoted collagen and elastic fiber formation through the TGF-β pathway.					
31036501	0	13	link	acid-cross-linked	11:27	arg1	filler					29:34	Hyaluronic acid-cross-linked filler	0:34	Hyaluronic acid-cross-linked filler	0:34	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	12	14	theme	collagen	1575:1582	arg1	type					1584:1587	collagen type 1	1575:1589	collagen type 1	1575:1589	HAc filler stimulates collagen type 1 and elastic fiber synthesis through the TGF-β/Smad pathway.					
31036501	0	15	theme	TGF-β/Smad	111:120	arg1	pathway					132:138	the TGF-β/Smad signaling pathway	107:138	the TGF-β/Smad signaling pathway in a nude mouse model	107:160	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	9	16	theme	gene	1199:1202	arg1	expression					1204:1213	gene expression	1199:1213	gene expression	1199:1213	Protein and gene expression analyses indicated that, compared with pure fillers, HAc-nano-HAp and HAc-micro-HAp hydrogels preferentially promoted collagen and elastic fiber formation through the TGF-β pathway.					
31036501	3	17	theme	biological	550:559	arg1	responses					561:569	the biological responses	546:569	the biological responses to HAc and HAp	546:584	Herein, we systematically compared the protein-level increase and gene expression between HAc-micro-HAp and HAc-nano-HAp in mice and determined the mechanisms underlying the biological responses to HAc and HAp.					
31036501	3	18	theme	gene	442:445	arg1	expression					447:456	gene expression	442:456	gene expression	442:456	Herein, we systematically compared the protein-level increase and gene expression between HAc-micro-HAp and HAc-nano-HAp in mice and determined the mechanisms underlying the biological responses to HAc and HAp.					
31036501	8	19	dep	Organ	1057:1061	arg1	liver					1064:1068	liver	1064:1068	liver	1064:1068	Organ (liver, lung, spleen, and kidney) toxicity of HAc-nano-HAp was determined by hematoxylin and eosin staining after 12 weeks.					
31036501	8	19	dep	Organ	1057:1061	arg1	spleen					1077:1082	spleen	1077:1082	spleen	1077:1082	Organ (liver, lung, spleen, and kidney) toxicity of HAc-nano-HAp was determined by hematoxylin and eosin staining after 12 weeks.					
31036501	8	19	dep	Organ	1057:1061	arg1	kidney					1089:1094	kidney	1089:1094	kidney	1089:1094	Organ (liver, lung, spleen, and kidney) toxicity of HAc-nano-HAp was determined by hematoxylin and eosin staining after 12 weeks.					
31036501	8	19	dep	Organ	1057:1061	arg1	lung					1071:1074	lung	1071:1074	lung	1071:1074	Organ (liver, lung, spleen, and kidney) toxicity of HAc-nano-HAp was determined by hematoxylin and eosin staining after 12 weeks.					
31036501	9	20	theme	elastic	1346:1352	arg1	formation					1360:1368	collagen and elastic fiber formation	1333:1368	formation	1360:1368	Protein and gene expression analyses indicated that, compared with pure fillers, HAc-nano-HAp and HAc-micro-HAp hydrogels preferentially promoted collagen and elastic fiber formation through the TGF-β pathway.					
31036501	6	21	theme	Skin	790:793	arg1	biopsies					795:802	Skin biopsies	790:802	Skin biopsies	790:802	Skin biopsies were performed to investigate collagen and elastic fiber synthesis after filler injections.					
31036501	10	22	theme	toxicity	1455:1462	arg1	evidence					1437:1444	no evidence	1434:1444	no evidence of organ toxicity	1434:1462	The composite fillers also exhibited no evidence of organ toxicity.					
31036501	0	23	theme	acid-cross-linked	11:27	arg1	filler					29:34	Hyaluronic acid-cross-linked filler	0:34	Hyaluronic acid-cross-linked filler	0:34	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	9	24	theme	fiber	1354:1358	arg1	formation					1360:1368	collagen and elastic fiber formation	1333:1368	formation	1360:1368	Protein and gene expression analyses indicated that, compared with pure fillers, HAc-nano-HAp and HAc-micro-HAp hydrogels preferentially promoted collagen and elastic fiber formation through the TGF-β pathway.					
31036501	8	25	dep	hematoxylin	1140:1150	arg1	staining					1162:1169	staining	1162:1169	staining	1162:1169	Organ (liver, lung, spleen, and kidney) toxicity of HAc-nano-HAp was determined by hematoxylin and eosin staining after 12 weeks.					
31036501	10	26	theme	organ	1449:1453	arg1	toxicity					1455:1462	organ toxicity	1449:1462	organ toxicity	1449:1462	The composite fillers also exhibited no evidence of organ toxicity.					
31036501	11	27	theme	HAc-HAp	1465:1471	arg1	filler					1473:1478	HAc-HAp filler	1465:1478	HAc-HAp filler	1465:1478	HAc-HAp filler might play an important role in collagen and elastic fiber regeneration.					
31036501	1	28	theme	pure	175:178	arg1	filler					196:201	pure hyaluronic acid filler	175:201	pure hyaluronic acid filler	175:201	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	0	29	theme	Hyaluronic	0:9	arg1	filler					29:34	Hyaluronic acid-cross-linked filler	0:34	Hyaluronic acid-cross-linked filler	0:34	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	7	30	theme	chain	940:944	arg1	reaction					946:953	real-time polymerase chain reaction	919:953	real-time polymerase chain reaction analysis	919:962	Western blot analysis, real-time polymerase chain reaction analysis, and immunohistochemistry were performed to investigate protein and gene expression changes.					
31036501	6	31	theme	elastic	847:853	arg1	synthesis					861:869	elastic fiber synthesis	847:869	elastic fiber synthesis	847:869	Skin biopsies were performed to investigate collagen and elastic fiber synthesis after filler injections.					
31036501	11	32	theme	important	1494:1502	arg1	role					1504:1507	an important role	1491:1507	an important role	1491:1507	HAc-HAp filler might play an important role in collagen and elastic fiber regeneration.					
31036501	1	33	theme	hyaluronic	180:189	arg1	filler					196:201	pure hyaluronic acid filler	175:201	pure hyaluronic acid filler	175:201	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	13	34	from	effects	1730:1736	arg1	photoaging					1692:1701	photoaging	1692:1701	photoaging	1692:1701	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	34	from	effects	1730:1736	arg1	models					1713:1718	animal models	1706:1718	animal models	1706:1718	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	34	from	effects	1730:1736	arg1	skin					1741:1744	skin	1741:1744	skin	1741:1744	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	7	35	theme	reaction	946:953	arg1	analysis					955:962	real-time polymerase chain reaction analysis	919:962	real-time polymerase chain reaction analysis	919:962	Western blot analysis, real-time polymerase chain reaction analysis, and immunohistochemistry were performed to investigate protein and gene expression changes.					
31036501	1	36	theme	acid	191:194	arg1	filler					196:201	pure hyaluronic acid filler	175:201	pure hyaluronic acid filler	175:201	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	4	37	theme	Five-week-old	587:599	arg1	mice					620:623	Five-week-old female BALB/c-nude mice	587:623	Five-week-old female BALB/c-nude mice	587:623	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	0	38	theme	signaling	122:130	arg1	pathway					132:138	the TGF-β/Smad signaling pathway	107:138	the TGF-β/Smad signaling pathway in a nude mouse model	107:160	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	6	39	theme	filler	877:882	arg1	injections					884:893	filler injections	877:893	filler injections	877:893	Skin biopsies were performed to investigate collagen and elastic fiber synthesis after filler injections.					
31036501	9	40	theme	TGF-β	1382:1386	arg1	pathway					1388:1394	the TGF-β pathway	1378:1394	the TGF-β pathway	1378:1394	Protein and gene expression analyses indicated that, compared with pure fillers, HAc-nano-HAp and HAc-micro-HAp hydrogels preferentially promoted collagen and elastic fiber formation through the TGF-β pathway.					
31036501	8	41	theme	HAc-nano-HAp	1109:1120	arg1	toxicity					1097:1104	Organ (liver, lung, spleen, and kidney) toxicity	1057:1104	Organ (liver, lung, spleen, and kidney) toxicity of HAc-nano-HAp	1057:1120	Organ (liver, lung, spleen, and kidney) toxicity of HAc-nano-HAp was determined by hematoxylin and eosin staining after 12 weeks.					
31036501	11	42	theme	fiber	1533:1537	arg1	regeneration					1539:1550	elastic fiber regeneration	1525:1550	elastic fiber regeneration	1525:1550	HAc-HAp filler might play an important role in collagen and elastic fiber regeneration.					
31036501	0	43	theme	collagen	47:54	arg1	type					56:59	collagen type 1	47:61	collagen type 1	47:61	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	1	44	theme	dermal	293:298	arg1	filler					300:305	a dermal filler	291:305	a dermal filler	291:305	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	1	44	theme	dermal	293:298	arg1	longevity					278:286	longevity	278:286	longevity	278:286	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	1	44	theme	dermal	293:298	arg1	biocompatibility					257:272	superior biocompatibility	248:272	superior biocompatibility	248:272	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	3	45	theme	protein-level	415:427	arg1	increase					429:436	the protein-level increase	411:436	the protein-level increase	411:436	Herein, we systematically compared the protein-level increase and gene expression between HAc-micro-HAp and HAc-nano-HAp in mice and determined the mechanisms underlying the biological responses to HAc and HAp.					
31036501	12	46	theme	HAc	1553:1555	arg1	filler					1557:1562	HAc filler	1553:1562	HAc filler	1553:1562	HAc filler stimulates collagen type 1 and elastic fiber synthesis through the TGF-β/Smad pathway.					
31036501	12	47	theme	fiber	1603:1607	arg1	synthesis					1609:1617	elastic fiber synthesis	1595:1617	elastic fiber synthesis	1595:1617	HAc filler stimulates collagen type 1 and elastic fiber synthesis through the TGF-β/Smad pathway.					
31036501	1	48	theme	cross-linked	204:215	arg1	HAc					234:236	HAc	234:236	HAc	234:236	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	1	48	theme	cross-linked	204:215	arg1	acid					228:231	cross-linked hyaluronic acid	204:231	cross-linked hyaluronic acid (HAc)	204:237	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	13	49	theme	tensile	1772:1778	arg1	strength					1780:1787	tensile strength	1772:1787	tensile strength	1772:1787	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	0	50	theme	mouse	150:154	arg1	model					156:160	a nude mouse model	143:160	a nude mouse model	143:160	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	6	51	theme	fiber	855:859	arg1	synthesis					861:869	elastic fiber synthesis	847:869	elastic fiber synthesis	847:869	Skin biopsies were performed to investigate collagen and elastic fiber synthesis after filler injections.					
31036501	10	52	theme	composite	1401:1409	arg1	fillers					1411:1417	The composite fillers	1397:1417	The composite fillers	1397:1417	The composite fillers also exhibited no evidence of organ toxicity.					
31036501	2	53	theme	HAc-hydroxyapatite	342:359	arg1	fillers					367:373	composite HAc-hydroxyapatite (HAp) fillers	332:373	composite HAc-hydroxyapatite (HAp) fillers	332:373	We previously developed composite HAc-hydroxyapatite (HAp) fillers.					
31036501	13	54	theme	animal	1706:1711	arg1	models					1713:1718	animal models	1706:1718	animal models	1706:1718	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	12	55	theme	TGF-β/Smad	1631:1640	arg1	pathway					1642:1648	the TGF-β/Smad pathway	1627:1648	the TGF-β/Smad pathway	1627:1648	HAc filler stimulates collagen type 1 and elastic fiber synthesis through the TGF-β/Smad pathway.					
31036501	0	56	theme	nude	145:148	arg1	model					156:160	a nude mouse model	143:160	a nude mouse model	143:160	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	4	57	dep	groups	651:656	arg1	skin					666:669	normal skin	659:669	normal skin	659:669	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	4	57	dep	groups	651:656	arg1	HAc-nano-HAp					693:704	HAc-nano-HAp	693:704	HAc-nano-HAp	693:704	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	4	57	dep	groups	651:656	arg1	HAc-micro-HAp					711:723	HAc-micro-HAp	711:723	HAc-micro-HAp	711:723	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	4	57	dep	groups	651:656	arg1	Radiesse					672:679	Radiesse	672:679	Radiesse	672:679	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	4	57	dep	groups	651:656	arg1	groups					651:656	five groups	646:656	five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp	646:723	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	4	57	dep	groups	651:656	arg1	Restylane					682:690	Restylane	682:690	Restylane	682:690	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	7	58	theme	gene	1032:1035	arg1	changes					1048:1054	protein and gene expression changes	1020:1054	changes	1048:1054	Western blot analysis, real-time polymerase chain reaction analysis, and immunohistochemistry were performed to investigate protein and gene expression changes.					
31036501	2	59	theme	composite	332:340	arg1	fillers					367:373	composite HAc-hydroxyapatite (HAp) fillers	332:373	composite HAc-hydroxyapatite (HAp) fillers	332:373	We previously developed composite HAc-hydroxyapatite (HAp) fillers.					
31036501	7	60	theme	real-time	919:927	arg1	reaction					946:953	real-time polymerase chain reaction	919:953	real-time polymerase chain reaction analysis	919:962	Western blot analysis, real-time polymerase chain reaction analysis, and immunohistochemistry were performed to investigate protein and gene expression changes.					
31036501	13	61	theme	composite	1671:1679	arg1	fillers					1681:1687	HAc-HAp composite fillers	1663:1687	HAc-HAp composite fillers in photoaging	1663:1701	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	9	62	theme	expression	1204:1213	arg1	analyses					1215:1222	Protein and gene expression analyses	1187:1222	Protein and gene expression analyses	1187:1222	Protein and gene expression analyses indicated that, compared with pure fillers, HAc-nano-HAp and HAc-micro-HAp hydrogels preferentially promoted collagen and elastic fiber formation through the TGF-β pathway.					
31036501	7	63	theme	expression	1037:1046	arg1	changes					1048:1054	protein and gene expression changes	1020:1054	changes	1048:1054	Western blot analysis, real-time polymerase chain reaction analysis, and immunohistochemistry were performed to investigate protein and gene expression changes.					
31036501	7	64	theme	polymerase	929:938	arg1	reaction					946:953	real-time polymerase chain reaction	919:953	real-time polymerase chain reaction analysis	919:962	Western blot analysis, real-time polymerase chain reaction analysis, and immunohistochemistry were performed to investigate protein and gene expression changes.					
31036501	1	65	theme	hyaluronic	217:226	arg1	HAc					234:236	HAc	234:236	HAc	234:236	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	1	65	theme	hyaluronic	217:226	arg1	acid					228:231	cross-linked hyaluronic acid	204:231	cross-linked hyaluronic acid (HAc)	204:237	Compared to pure hyaluronic acid filler, cross-linked hyaluronic acid (HAc) exhibits superior biocompatibility and longevity as a dermal filler.					
31036501	13	66	theme	fillers	1681:1687	arg1	elasticity					1757:1766	elasticity	1757:1766	elasticity	1757:1766	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	66	theme	fillers	1681:1687	arg1	effects					1730:1736	their effects	1724:1736	their effects	1724:1736	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	66	theme	fillers	1681:1687	arg1	strength					1780:1787	tensile strength	1772:1787	tensile strength	1772:1787	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	66	theme	fillers	1681:1687	arg1	role					1655:1658	The role	1651:1658	The role of HAc-HAp composite fillers in photoaging in animal models	1651:1718	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	3	67	from	increase	429:436	arg1	mice					500:503	mice	500:503	mice	500:503	Herein, we systematically compared the protein-level increase and gene expression between HAc-micro-HAp and HAc-nano-HAp in mice and determined the mechanisms underlying the biological responses to HAc and HAp.					
31036501	13	68	from	photoaging	1692:1701	arg1	elasticity					1757:1766	elasticity	1757:1766	elasticity	1757:1766	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	68	from	photoaging	1692:1701	arg1	effects					1730:1736	their effects	1724:1736	their effects	1724:1736	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	68	from	photoaging	1692:1701	arg1	strength					1780:1787	tensile strength	1772:1787	tensile strength	1772:1787	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	68	from	photoaging	1692:1701	arg1	role					1655:1658	The role	1651:1658	The role of HAc-HAp composite fillers in photoaging in animal models	1651:1718	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	13	69	from	fillers	1681:1687	arg1	photoaging					1692:1701	photoaging	1692:1701	photoaging	1692:1701	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	0	70	theme	fiber	75:79	arg1	synthesis					81:89	elastic fiber synthesis	67:89	elastic fiber synthesis	67:89	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	4	71	theme	normal	659:664	arg1	skin					666:669	normal skin	659:669	normal skin	659:669	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	4	71	theme	normal	659:664	arg1	groups					651:656	five groups	646:656	five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp	646:723	Five-week-old female BALB/c-nude mice were classified into five groups: normal skin, Radiesse, Restylane, HAc-nano-HAp, and HAc-micro-HAp.					
31036501	11	72	theme	elastic	1525:1531	arg1	regeneration					1539:1550	elastic fiber regeneration	1525:1550	elastic fiber regeneration	1525:1550	HAc-HAp filler might play an important role in collagen and elastic fiber regeneration.					
31036501	0	73	from	pathway	132:138	arg1	model					156:160	a nude mouse model	143:160	a nude mouse model	143:160	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	0	74	theme	elastic	67:73	arg1	synthesis					81:89	elastic fiber synthesis	67:89	elastic fiber synthesis	67:89	Hyaluronic acid-cross-linked filler stimulates collagen type 1 and elastic fiber synthesis in skin through the TGF-β/Smad signaling pathway in a nude mouse model.					
31036501	9	75	theme	HAc-micro-HAp	1285:1297	arg1	hydrogels					1299:1307	HAc-micro-HAp hydrogels	1285:1307	HAc-micro-HAp hydrogels	1285:1307	Protein and gene expression analyses indicated that, compared with pure fillers, HAc-nano-HAp and HAc-micro-HAp hydrogels preferentially promoted collagen and elastic fiber formation through the TGF-β pathway.					
31036501	13	76	theme	HAc-HAp	1663:1669	arg1	fillers					1681:1687	HAc-HAp composite fillers	1663:1687	HAc-HAp composite fillers in photoaging	1663:1701	The role of HAc-HAp composite fillers in photoaging in animal models and their effects on skin, including elasticity and tensile strength, should be investigated.					
31036501	7	77	theme	Western	896:902	arg1	analysis					909:916	Western blot analysis	896:916	Western blot analysis	896:916	Western blot analysis, real-time polymerase chain reaction analysis, and immunohistochemistry were performed to investigate protein and gene expression changes.					
31036501	12	78	theme	elastic	1595:1601	arg1	synthesis					1609:1617	elastic fiber synthesis	1595:1617	elastic fiber synthesis	1595:1617	HAc filler stimulates collagen type 1 and elastic fiber synthesis through the TGF-β/Smad pathway.					
30543886	0	0	theme	ultrasound	79:88	arg1	method					99:104	ultrasound assisted method	79:104	ultrasound assisted method	79:104	Optimization and characterization of pectin extracted from sour orange peel by ultrasound assisted method.					
30543886	2	1	theme	maximum	318:324	arg1	%					359:359	28.07 ± 0.67%	347:359	28.07 ± 0.67%	347:359	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	2	1	theme	maximum	318:324	arg1	yield					337:341	the maximum extraction yield	314:341	the maximum extraction yield	314:341	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	2	2	theme	1.5	428:430	arg1	pH					422:423	pH	422:423	pH	422:423	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	2	2	theme	1.5	428:430	arg1	10 min					411:416	10 min	411:416	10 min	411:416	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	5	3	theme	pectin	820:825	arg1	pectin					1020:1025	oil/g pectin	1014:1025	oil/g pectin	1014:1025	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	3	theme	pectin	820:825	arg1	capacity					963:970	oil holding capacity	951:970	oil holding capacity	951:970	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	3	theme	pectin	820:825	arg1	capacity					938:945	water holding capacity	924:945	water holding capacity	924:945	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	3	theme	pectin	820:825	arg1	tension					840:846	the surface tension	828:846	the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v	828:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	3	theme	pectin	820:825	arg1	content					767:773	a total phenolic content	750:773	a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin	750:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	4	from	concentrations	889:902	arg1	pectin					1020:1025	oil/g pectin	1014:1025	oil/g pectin	1014:1025	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	4	from	concentrations	889:902	arg1	capacity					963:970	oil holding capacity	951:970	oil holding capacity	951:970	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	4	from	concentrations	889:902	arg1	capacity					938:945	water holding capacity	924:945	water holding capacity	924:945	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	4	from	concentrations	889:902	arg1	tension					840:846	the surface tension	828:846	the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v	828:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	4	from	concentrations	889:902	arg1	content					767:773	a total phenolic content	750:773	a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin	750:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	5	theme	%	918:918	arg1	w/v					919:921	0.1 and 0.5%w/v	907:921	w/v	919:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	6	from	42.14 ± 0.61 mN/m	868:884	arg1	concentrations					889:902	concentrations	889:902	concentrations of 0.1 and 0.5%w/v	889:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	7	theme	equivalents/g	806:818	arg1	pectin					820:825	39.95 ± 3.13 mg gallic acid equivalents/g pectin	778:825	39.95 ± 3.13 mg gallic acid equivalents/g pectin	778:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	0	8	theme	assisted	90:97	arg1	method					99:104	ultrasound assisted method	79:104	ultrasound assisted method	79:104	Optimization and characterization of pectin extracted from sour orange peel by ultrasound assisted method.					
30543886	1	9	with	design	135:140	arg1	variables					159:167	three variables	153:167	three variables (ultrasound power, irradiation time and pH) in three levels	153:227	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	9	with	design	135:140	arg1	power					181:185	ultrasound power	170:185	ultrasound power	170:185	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	9	with	design	135:140	arg1	time					200:203	irradiation time	188:203	irradiation time	188:203	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	9	with	design	135:140	arg1	pH					209:210	pH	209:210	pH	209:210	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	6	10	theme	esterification	1116:1129	arg1	degree					1106:1111	degree	1106:1111	degree of esterification of 6.77 ± 0.43%	1106:1145	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	5	11	theme	0.5	915:917	arg1	w/v					919:921	0.1 and 0.5%w/v	907:921	w/v	919:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	4	12	theme	pectin	635:640	arg1	%					616:616	65.3%	612:616	65.3% of the extracted pectin	612:640	HPLC analysis indicated that 65.3% of the extracted pectin was galacturonic acid and approximately 72% of total neutral sugars was galactose.					
30543886	4	12	theme	pectin	635:640	arg1	pectin					635:640	the extracted pectin	621:640	the extracted pectin	621:640	HPLC analysis indicated that 65.3% of the extracted pectin was galacturonic acid and approximately 72% of total neutral sugars was galactose.					
30543886	5	13	theme	oil/g	1014:1018	arg1	pectin					1020:1025	oil/g pectin	1014:1025	oil/g pectin	1014:1025	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	4	14	theme	total	689:693	arg1	sugars					703:708	total neutral sugars	689:708	total neutral sugars	689:708	HPLC analysis indicated that 65.3% of the extracted pectin was galacturonic acid and approximately 72% of total neutral sugars was galactose.					
30543886	4	15	theme	HPLC	583:586	arg1	analysis					588:595	HPLC analysis	583:595	HPLC analysis	583:595	HPLC analysis indicated that 65.3% of the extracted pectin was galacturonic acid and approximately 72% of total neutral sugars was galactose.					
30543886	6	16	theme	methoxyl	1169:1176	arg1	pectin					1178:1183	low methoxyl pectin	1165:1183	low methoxyl pectin	1165:1183	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	6	16	theme	methoxyl	1169:1176	arg1	SOPP					1096:1099	SOPP	1096:1099	SOPP with degree of esterification of 6.77 ± 0.43%	1096:1145	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	5	17	from	tension	840:846	arg1	concentrations					889:902	concentrations	889:902	concentrations of 0.1 and 0.5%w/v	889:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	18	theme	holding	930:936	arg1	capacity					938:945	water holding capacity	924:945	water holding capacity	924:945	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	6	19	with	SOPP	1096:1099	arg1	degree					1106:1111	degree	1106:1111	degree of esterification of 6.77 ± 0.43%	1106:1145	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	6	20	theme	low	1165:1167	arg1	pectin					1178:1183	low methoxyl pectin	1165:1183	low methoxyl pectin	1165:1183	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	6	20	theme	low	1165:1167	arg1	SOPP					1096:1099	SOPP	1096:1099	SOPP with degree of esterification of 6.77 ± 0.43%	1096:1145	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	5	21	from	capacity	963:970	arg1	concentrations					889:902	concentrations	889:902	concentrations of 0.1 and 0.5%w/v	889:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	22	theme	46.56 ± 0.23	851:862	arg1	pectin					1020:1025	oil/g pectin	1014:1025	oil/g pectin	1014:1025	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	22	theme	46.56 ± 0.23	851:862	arg1	capacity					963:970	oil holding capacity	951:970	oil holding capacity	951:970	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	22	theme	46.56 ± 0.23	851:862	arg1	capacity					938:945	water holding capacity	924:945	water holding capacity	924:945	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	22	theme	46.56 ± 0.23	851:862	arg1	tension					840:846	the surface tension	828:846	the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v	828:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	22	theme	46.56 ± 0.23	851:862	arg1	content					767:773	a total phenolic content	750:773	a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin	750:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	23	theme	39.95 ± 3.13 mg	778:792	arg1	acid					801:804	39.95 ± 3.13 mg gallic acid	778:804	39.95 ± 3.13 mg gallic acid equivalents/g pectin	778:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	3	24	theme	protein	496:502	arg1	contents					504:511	protein contents	496:511	protein contents	496:511	In these conditions, ash, moisture and protein contents of SOPP were 1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%, respectively.					
30543886	5	25	from	capacity	938:945	arg1	concentrations					889:902	concentrations	889:902	concentrations of 0.1 and 0.5%w/v	889:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	26	theme	gallic	794:799	arg1	acid					801:804	39.95 ± 3.13 mg gallic acid	778:804	39.95 ± 3.13 mg gallic acid equivalents/g pectin	778:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	2	27	theme	irradiation	391:401	arg1	time					403:406	irradiation time	391:406	irradiation time of 10 min and pH of 1.5 (as optimum conditions)	391:454	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	2	27	theme	irradiation	391:401	arg1	150 W					384:388	150 W	384:388	150 W	384:388	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	5	28	theme	w/v	919:921	arg1	concentrations					889:902	concentrations	889:902	concentrations of 0.1 and 0.5%w/v	889:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	29	contain	had	746:748	arg2	content					767:773	a total phenolic content	750:773	a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin	750:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	29	contain	had	746:748	arg2	tension					840:846	the surface tension	828:846	the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v	828:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	29	contain	had	746:748	arg2	pectin					1020:1025	oil/g pectin	1014:1025	oil/g pectin	1014:1025	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	29	contain	had	746:748	arg2	capacity					938:945	water holding capacity	924:945	water holding capacity	924:945	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	29	contain	had	746:748	arg1	pectin					739:744	The optimized pectin	725:744	The optimized pectin	725:744	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	30	theme	42.14 ± 0.61 mN/m	868:884	arg1	pectin					1020:1025	oil/g pectin	1014:1025	oil/g pectin	1014:1025	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	30	theme	42.14 ± 0.61 mN/m	868:884	arg1	capacity					963:970	oil holding capacity	951:970	oil holding capacity	951:970	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	30	theme	42.14 ± 0.61 mN/m	868:884	arg1	capacity					938:945	water holding capacity	924:945	water holding capacity	924:945	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	30	theme	42.14 ± 0.61 mN/m	868:884	arg1	tension					840:846	the surface tension	828:846	the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v	828:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	30	theme	42.14 ± 0.61 mN/m	868:884	arg1	content					767:773	a total phenolic content	750:773	a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin	750:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	31	theme	water	924:928	arg1	capacity					938:945	water holding capacity	924:945	water holding capacity	924:945	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	2	32	theme	150 W	384:388	arg1	power					375:379	ultrasound power	364:379	ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions)	364:454	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	4	33	theme	galacturonic	646:657	arg1	acid					659:662	galacturonic acid	646:662	galacturonic acid	646:662	HPLC analysis indicated that 65.3% of the extracted pectin was galacturonic acid and approximately 72% of total neutral sugars was galactose.					
30543886	5	34	theme	0.1	907:909	arg1	w/v					919:921	0.1 and 0.5%w/v	907:921	w/v	919:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	35	theme	optimized	729:737	arg1	pectin					739:744	The optimized pectin	725:744	The optimized pectin	725:744	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	1	36	theme	ultrasound	170:179	arg1	variables					159:167	three variables	153:167	three variables (ultrasound power, irradiation time and pH) in three levels	153:227	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	36	theme	ultrasound	170:179	arg1	power					181:185	ultrasound power	170:185	ultrasound power	170:185	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	37	theme	pectin	245:250	arg1	optimization					263:274	pectin extraction optimization	245:274	pectin extraction optimization	245:274	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	3	38	theme	SOPP	516:519	arg1	moisture					483:490	moisture	483:490	moisture	483:490	In these conditions, ash, moisture and protein contents of SOPP were 1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%, respectively.					
30543886	3	38	theme	SOPP	516:519	arg1	contents					504:511	protein contents	496:511	protein contents	496:511	In these conditions, ash, moisture and protein contents of SOPP were 1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%, respectively.					
30543886	3	38	theme	SOPP	516:519	arg1	ash					478:480	ash	478:480	ash	478:480	In these conditions, ash, moisture and protein contents of SOPP were 1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%, respectively.					
30543886	5	39	theme	total	752:756	arg1	content					767:773	a total phenolic content	750:773	a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin	750:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	2	40	theme	pH	422:423	arg1	time					403:406	irradiation time	391:406	irradiation time of 10 min and pH of 1.5 (as optimum conditions)	391:454	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	2	40	theme	pH	422:423	arg1	150 W					384:388	150 W	384:388	150 W	384:388	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	1	41	theme	extraction	252:261	arg1	optimization					263:274	pectin extraction optimization	245:274	pectin extraction optimization	245:274	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	0	42	theme	pectin	37:42	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Optimization and characterization of pectin extracted from sour orange peel by ultrasound assisted method.					
30543886	0	42	theme	pectin	37:42	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization and characterization of pectin extracted from sour orange peel by ultrasound assisted method.					
30543886	3	43	theme	1.45 ± 0.23	555:565	arg1	%					566:566	1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%	526:566	%	566:566	In these conditions, ash, moisture and protein contents of SOPP were 1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%, respectively.					
30543886	1	44	dep	variables	159:167	arg1	pH					209:210	pH	209:210	pH	209:210	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	44	dep	variables	159:167	arg1	power					181:185	ultrasound power	170:185	ultrasound power	170:185	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	44	dep	variables	159:167	arg1	time					200:203	irradiation time	188:203	irradiation time	188:203	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	44	dep	variables	159:167	arg1	variables					159:167	three variables	153:167	three variables (ultrasound power, irradiation time and pH) in three levels	153:227	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	2	45	theme	ultrasound	364:373	arg1	power					375:379	ultrasound power	364:379	ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions)	364:454	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	0	46	theme	sour	59:62	arg1	peel					71:74	sour orange peel	59:74	sour orange peel	59:74	Optimization and characterization of pectin extracted from sour orange peel by ultrasound assisted method.					
30543886	5	47	theme	acid	801:804	arg1	pectin					820:825	39.95 ± 3.13 mg gallic acid equivalents/g pectin	778:825	39.95 ± 3.13 mg gallic acid equivalents/g pectin	778:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	6	48	theme	NMR	1217:1219	arg1	analysis					1221:1228	FTIR and 1H NMR analysis	1205:1228	analysis	1221:1228	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	5	49	theme	water	1005:1009	arg1	pectin					1020:1025	oil/g pectin	1014:1025	oil/g pectin	1014:1025	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	49	theme	water	1005:1009	arg1	capacity					963:970	oil holding capacity	951:970	oil holding capacity	951:970	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	49	theme	water	1005:1009	arg1	capacity					938:945	water holding capacity	924:945	water holding capacity	924:945	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	49	theme	water	1005:1009	arg1	tension					840:846	the surface tension	828:846	the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v	828:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	49	theme	water	1005:1009	arg1	content					767:773	a total phenolic content	750:773	a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin	750:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	6	50	theme	%	1145:1145	arg1	esterification					1116:1129	esterification	1116:1129	esterification of 6.77 ± 0.43%	1116:1145	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	4	51	theme	extracted	625:633	arg1	pectin					635:640	the extracted pectin	621:640	the extracted pectin	621:640	HPLC analysis indicated that 65.3% of the extracted pectin was galacturonic acid and approximately 72% of total neutral sugars was galactose.					
30543886	2	52	theme	10 min	411:416	arg1	time					403:406	irradiation time	391:406	irradiation time of 10 min and pH of 1.5 (as optimum conditions)	391:454	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	2	52	theme	10 min	411:416	arg1	150 W					384:388	150 W	384:388	150 W	384:388	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	6	53	theme	1H	1214:1215	arg1	analysis					1221:1228	FTIR and 1H NMR analysis	1205:1228	analysis	1221:1228	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	5	54	theme	oil	951:953	arg1	capacity					963:970	oil holding capacity	951:970	oil holding capacity	951:970	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	55	theme	antioxidant	1059:1069	arg1	properties					1071:1080	antioxidant properties	1059:1080	antioxidant properties	1059:1080	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	56	theme	surface	832:838	arg1	tension					840:846	the surface tension	828:846	the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v	828:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	57	from	pectin	1020:1025	arg1	concentrations					889:902	concentrations	889:902	concentrations of 0.1 and 0.5%w/v	889:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	5	58	theme	holding	955:961	arg1	capacity					963:970	oil holding capacity	951:970	oil holding capacity	951:970	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	4	59	theme	sugars	703:708	arg1	%					684:684	approximately 72%	668:684	approximately 72% of total neutral sugars	668:708	HPLC analysis indicated that 65.3% of the extracted pectin was galacturonic acid and approximately 72% of total neutral sugars was galactose.					
30543886	4	59	theme	sugars	703:708	arg1	sugars					703:708	total neutral sugars	689:708	total neutral sugars	689:708	HPLC analysis indicated that 65.3% of the extracted pectin was galacturonic acid and approximately 72% of total neutral sugars was galactose.					
30543886	2	60	from	%	359:359	arg1	power					375:379	ultrasound power	364:379	ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions)	364:454	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	1	61	theme	irradiation	188:198	arg1	variables					159:167	three variables	153:167	three variables (ultrasound power, irradiation time and pH) in three levels	153:227	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	61	theme	irradiation	188:198	arg1	time					200:203	irradiation time	188:203	irradiation time	188:203	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	6	62	theme	FTIR	1205:1208	arg1	analysis					1221:1228	FTIR and 1H NMR analysis	1205:1228	analysis	1221:1228	In addition, SOPP with degree of esterification of 6.77 ± 0.43% was classified as low methoxyl pectin, which confirmed by FTIR and 1H NMR analysis.					
30543886	4	63	theme	neutral	695:701	arg1	sugars					703:708	total neutral sugars	689:708	total neutral sugars	689:708	HPLC analysis indicated that 65.3% of the extracted pectin was galacturonic acid and approximately 72% of total neutral sugars was galactose.					
30543886	2	64	theme	optimization	281:292	arg1	process					294:300	The optimization process	277:300	The optimization process	277:300	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	5	65	from	46.56 ± 0.23	851:862	arg1	concentrations					889:902	concentrations	889:902	concentrations of 0.1 and 0.5%w/v	889:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	2	66	theme	optimum	436:442	arg1	conditions					444:453	optimum conditions	436:453	optimum conditions	436:453	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	5	67	theme	phenolic	758:765	arg1	content					767:773	a total phenolic content	750:773	a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin	750:825	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	3	68	theme	1.89 ± 0.51	526:536	arg1	%					566:566	1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%	526:566	%	566:566	In these conditions, ash, moisture and protein contents of SOPP were 1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%, respectively.					
30543886	1	69	from	variables	159:167	arg1	levels					222:227	three levels	216:227	three levels	216:227	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	70	theme	Box-Behnken	123:133	arg1	design					135:140	a Box-Behnken design	121:140	a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels	121:227	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	1	70	theme	Box-Behnken	123:133	arg1	BBD					143:145	BBD	143:145	BBD	143:145	In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization.					
30543886	0	71	theme	orange	64:69	arg1	peel					71:74	sour orange peel	59:74	sour orange peel	59:74	Optimization and characterization of pectin extracted from sour orange peel by ultrasound assisted method.					
30543886	5	72	from	content	767:773	arg1	concentrations					889:902	concentrations	889:902	concentrations of 0.1 and 0.5%w/v	889:921	The optimized pectin had a total phenolic content of 39.95 ± 3.13 mg gallic acid equivalents/g pectin, the surface tension of 46.56 ± 0.23 and 42.14 ± 0.61 mN/m in concentrations of 0.1 and 0.5%w/v, water holding capacity and oil holding capacity of 3.10 ± 0.12 and 1.32 ± 0.21 g water or oil/g pectin with a suitable emulsifying and antioxidant properties.					
30543886	2	73	theme	extraction	326:335	arg1	%					359:359	28.07 ± 0.67%	347:359	28.07 ± 0.67%	347:359	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	2	73	theme	extraction	326:335	arg1	yield					337:341	the maximum extraction yield	314:341	the maximum extraction yield	314:341	The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions).					
30543886	3	74	from	%	566:566	arg1	conditions					466:475	these conditions	460:475	these conditions	460:475	In these conditions, ash, moisture and protein contents of SOPP were 1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%, respectively.					
31321622	6	0	theme	>	1209:1209	arg1	concentrations					1193:1206	the highest concentrations	1181:1206	the highest concentrations (> 1 g/100 g DW)	1181:1223	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	6	0	theme	>	1209:1209	arg1	DW					1221:1222	> 1 g/100 g DW	1209:1222	> 1 g/100 g DW	1209:1222	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	3	1	theme	parts	582:586	arg1	composition					546:556	the composition	542:556	the composition of the VO morphological parts	542:586	The results showed great quantitative differences in the composition of the VO morphological parts tested.					
31321622	4	2	theme	6.82 ± 0.38 g/100 g	735:753	arg1	DW					755:756	6.82 ± 0.38 g/100 g DW	735:756	6.82 ± 0.38 g/100 g DW	735:756	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	6	3	theme	1 g/100 g	1211:1219	arg1	concentrations					1193:1206	the highest concentrations	1181:1206	the highest concentrations (> 1 g/100 g DW)	1181:1223	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	6	3	theme	1 g/100 g	1211:1219	arg1	DW					1221:1222	> 1 g/100 g DW	1209:1222	> 1 g/100 g DW	1209:1222	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	1	4	theme	antioxidant	255:265	arg1	capacity					267:274	antioxidant capacity	255:274	antioxidant capacity	255:274	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	2	5	theme	in	474:475	arg1	test					483:486	in vitro test	474:486	in vitro test	474:486	Antioxidant capacity was evaluated against ABTS, hydroxyl, peroxyl and superoxide free radicals, and as a reducing power by using in vitro test.					
31321622	1	6	theme	vitamin	202:208	arg1	C					210:210	vitamin C	202:210	vitamin C	202:210	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	2	7	theme	superoxide	415:424	arg1	radicals					431:438	peroxyl and superoxide free radicals	403:438	radicals	431:438	Antioxidant capacity was evaluated against ABTS, hydroxyl, peroxyl and superoxide free radicals, and as a reducing power by using in vitro test.					
31321622	1	8	theme	capacity	267:274	arg1	profiles					124:131	the profiles	120:131	the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity	120:274	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	1	9	theme	C	210:210	arg1	profiles					124:131	the profiles	120:131	the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity	120:274	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	6	10	theme	highest	1185:1191	arg1	concentrations					1193:1206	the highest concentrations	1181:1206	the highest concentrations (> 1 g/100 g DW)	1181:1223	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	6	10	theme	highest	1185:1191	arg1	DW					1221:1222	> 1 g/100 g DW	1209:1222	> 1 g/100 g DW	1209:1222	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	5	11	theme	total	937:941	arg1	terms					876:880	terms	876:880	terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents	876:1030	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	4	12	theme	fat	643:645	arg1	concentrations					625:638	the highest concentrations	613:638	the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW)	613:813	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	2	13	theme	peroxyl	403:409	arg1	radicals					431:438	peroxyl and superoxide free radicals	403:438	radicals	431:438	Antioxidant capacity was evaluated against ABTS, hydroxyl, peroxyl and superoxide free radicals, and as a reducing power by using in vitro test.					
31321622	4	14	contain	contained	603:611	arg1	Fruits					596:601	Fruits	596:601	Fruits	596:601	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	4	14	contain	contained	603:611	arg2	concentrations					625:638	the highest concentrations	613:638	the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW)	613:813	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	4	15	theme	acids	656:660	arg1	concentrations					625:638	the highest concentrations	613:638	the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW)	613:813	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	5	16	from	VO	870:871	arg1	terms					876:880	terms	876:880	terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents	876:1030	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	1	17	theme	phenolics	136:144	arg1	profiles					124:131	the profiles	120:131	the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity	120:274	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	2	18	theme	Antioxidant	344:354	arg1	capacity					356:363	Antioxidant capacity	344:363	Antioxidant capacity	344:363	Antioxidant capacity was evaluated against ABTS, hydroxyl, peroxyl and superoxide free radicals, and as a reducing power by using in vitro test.					
31321622	6	19	theme	phenolic	1098:1105	arg1	compounds					1107:1115	the phenolic compounds	1094:1115	the phenolic compounds quantified in this study	1094:1140	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	6	20	contain	had	1177:1179	arg1	acid					1155:1158	chlorogenic acid	1143:1158	chlorogenic acid	1143:1158	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	6	20	contain	had	1177:1179	arg2	DW					1221:1222	> 1 g/100 g DW	1209:1222	> 1 g/100 g DW	1209:1222	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	6	20	contain	had	1177:1179	arg2	concentrations					1193:1206	the highest concentrations	1181:1206	the highest concentrations (> 1 g/100 g DW)	1181:1223	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	6	20	contain	had	1177:1179	arg1	-catechin					1167:1175	(+)-catechin	1164:1175	(+)-catechin	1164:1175	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	4	21	theme	organic	648:654	arg1	acids					656:660	organic acids	648:660	organic acids	648:660	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	5	22	from	parts	857:861	arg1	terms					876:880	terms	876:880	terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents	876:1030	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	0	23	theme	opulus	98:103	arg1	Flower					70:75	Flower	70:75	Flower	70:75	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	0	23	theme	opulus	98:103	arg1	Comparison					0:9	Comparison	0:9	Comparison of Chemical Composition and Antioxidant Capacity of Fruit	0:67	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	0	23	theme	opulus	98:103	arg1	Bark					81:84	Bark	81:84	Bark	81:84	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	4	24	theme	highest	617:623	arg1	concentrations					625:638	the highest concentrations	613:638	the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW)	613:813	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	1	25	theme	fiber	147:151	arg1	profiles					124:131	the profiles	120:131	the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity	120:274	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	5	26	theme	insoluble	973:981	arg1	fiber					991:995	insoluble dietary fiber	973:995	insoluble dietary fiber (58.20 ± 0.73 g/100 g DW)	973:1021	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	26	theme	insoluble	973:981	arg1	DW					1019:1020	58.20 ± 0.73 g/100 g DW	998:1020	58.20 ± 0.73 g/100 g DW	998:1020	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	1	27	theme	ash	213:215	arg1	profiles					124:131	the profiles	120:131	the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity	120:274	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	5	28	theme	dietary	983:989	arg1	fiber					991:995	insoluble dietary fiber	973:995	insoluble dietary fiber (58.20 ± 0.73 g/100 g DW)	973:1021	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	28	theme	dietary	983:989	arg1	DW					1019:1020	58.20 ± 0.73 g/100 g DW	998:1020	58.20 ± 0.73 g/100 g DW	998:1020	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	0	29	theme	Composition	23:33	arg1	Flower					70:75	Flower	70:75	Flower	70:75	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	0	29	theme	Composition	23:33	arg1	Comparison					0:9	Comparison	0:9	Comparison of Chemical Composition and Antioxidant Capacity of Fruit	0:67	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	0	29	theme	Composition	23:33	arg1	Bark					81:84	Bark	81:84	Bark	81:84	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	5	30	from	compounds	1052:1060	arg1	terms					876:880	terms	876:880	terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents	876:1030	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	1	31	theme	pectins	154:160	arg1	profiles					124:131	the profiles	120:131	the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity	120:274	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	0	32	theme	Chemical	14:21	arg1	Composition					23:33	Chemical Composition	14:33	Chemical Composition	14:33	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	1	33	theme	protein	218:224	arg1	profiles					124:131	the profiles	120:131	the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity	120:274	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	3	34	theme	quantitative	514:525	arg1	differences					527:537	great quantitative differences	508:537	great quantitative differences in the composition of the VO morphological parts	508:586	The results showed great quantitative differences in the composition of the VO morphological parts tested.					
31321622	0	35	theme	Antioxidant	39:49	arg1	Capacity					51:58	Antioxidant Capacity	39:58	Antioxidant Capacity	39:58	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	6	36	dep	flowers	1232:1238	arg1	the					1228:1230	the	1228:1230	the	1228:1230	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	4	37	theme	fiber	687:691	arg1	concentrations					625:638	the highest concentrations	613:638	the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW)	613:813	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	5	38	from	terms	876:880	arg1	compounds					1052:1060	phenolic compounds	1043:1060	the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW)	843:1085	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	38	from	terms	876:880	arg1	DW					1083:1084	3.98 ± 0.04 g/100 g DW	1063:1084	3.98 ± 0.04 g/100 g DW	1063:1084	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	38	from	terms	876:880	arg1	parts					857:861	the remaining parts	843:861	the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW)	843:1085	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	4	39	dep	fiber	687:691	arg1	10.57 ± 0.54					694:705	10.57 ± 0.54	694:705	10.57 ± 0.54	694:705	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	1	40	theme	fat	230:232	arg1	contents					234:241	fat contents	230:241	fat contents	230:241	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	2	41	theme	free	426:429	arg1	radicals					431:438	peroxyl and superoxide free radicals	403:438	radicals	431:438	Antioxidant capacity was evaluated against ABTS, hydroxyl, peroxyl and superoxide free radicals, and as a reducing power by using in vitro test.					
31321622	4	42	theme	dietary	679:685	arg1	fiber					687:691	soluble dietary fiber	671:691	soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively)	671:771	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	3	43	from	differences	527:537	arg1	composition					546:556	the composition	542:556	the composition of the VO morphological parts	542:586	The results showed great quantitative differences in the composition of the VO morphological parts tested.					
31321622	6	44	dep	-catechin	1167:1175	arg1	+					1165:1165	+	1165:1165	+	1165:1165	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	1	45	theme	contents	234:241	arg1	profiles					124:131	the profiles	120:131	the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity	120:274	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	3	46	theme	great	508:512	arg1	differences					527:537	great quantitative differences	508:537	great quantitative differences in the composition of the VO morphological parts	508:586	The results showed great quantitative differences in the composition of the VO morphological parts tested.					
31321622	6	47	theme	chlorogenic	1143:1153	arg1	acid					1155:1158	chlorogenic acid	1143:1158	chlorogenic acid	1143:1158	Among the phenolic compounds quantified in this study, chlorogenic acid and (+)-catechin had the highest concentrations (> 1 g/100 g DW) in the flowers and bark, respectively.					
31321622	0	48	theme	Capacity	51:58	arg1	Flower					70:75	Flower	70:75	Flower	70:75	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	0	48	theme	Capacity	51:58	arg1	Comparison					0:9	Comparison	0:9	Comparison of Chemical Composition and Antioxidant Capacity of Fruit	0:67	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	0	48	theme	Capacity	51:58	arg1	Bark					81:84	Bark	81:84	Bark	81:84	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	2	49	theme	reducing	450:457	arg1	power					459:463	as a reducing power	445:463	as a reducing power	445:463	Antioxidant capacity was evaluated against ABTS, hydroxyl, peroxyl and superoxide free radicals, and as a reducing power by using in vitro test.					
31321622	5	50	theme	capacity	897:904	arg1	terms					876:880	terms	876:880	terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents	876:1030	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	51	theme	antioxidant	885:895	arg1	capacity					897:904	antioxidant capacity	885:904	antioxidant capacity	885:904	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	52	theme	fiber	991:995	arg1	terms					876:880	terms	876:880	terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents	876:1030	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	3	53	theme	morphological	568:580	arg1	parts					582:586	the VO morphological parts	561:586	the VO morphological parts	561:586	The results showed great quantitative differences in the composition of the VO morphological parts tested.					
31321622	4	54	theme	carotenoids	777:787	arg1	concentrations					625:638	the highest concentrations	613:638	the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW)	613:813	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	4	55	theme	sugars	663:668	arg1	concentrations					625:638	the highest concentrations	613:638	the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW)	613:813	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	1	56	theme	organic	171:177	arg1	acids					179:183	organic acids	171:183	organic acids	171:183	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	5	57	theme	remaining	847:855	arg1	parts					857:861	the remaining parts	843:861	the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW)	843:1085	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	58	theme	ash	907:909	arg1	terms					876:880	terms	876:880	terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents	876:1030	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	59	dep	exceeded	834:841	arg1	Whereas					816:822	Whereas	816:822	Whereas	816:822	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	1	60	theme	opulus	331:336	arg1	flowers					301:307	flowers	301:307	flowers	301:307	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	1	60	theme	opulus	331:336	arg1	bark					314:317	bark	314:317	bark of Viburnum opulus (VO)	314:341	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	1	60	theme	opulus	331:336	arg1	fruits					293:298	fruits	293:298	fruits	293:298	In this work, the profiles of phenolics, fiber, pectins, sugars, organic acids and carotenoids, vitamin C, ash, protein and fat contents, as well as antioxidant capacity were compared in fruits, flowers, and bark of Viburnum opulus (VO).					
31321622	4	61	theme	soluble	671:677	arg1	fiber					687:691	soluble dietary fiber	671:691	soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively)	671:771	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	4	62	dep	10.57 ± 0.54	694:705	arg1	32.27 ± 1.25					721:732	32.27 ± 1.25	721:732	32.27 ± 1.25	721:732	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	4	62	dep	10.57 ± 0.54	694:705	arg1	DW					755:756	6.82 ± 0.38 g/100 g DW	735:756	6.82 ± 0.38 g/100 g DW	735:756	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	4	62	dep	10.57 ± 0.54	694:705	arg1	7.34 ± 0.06					708:718	7.34 ± 0.06	708:718	7.34 ± 0.06	708:718	Fruits contained the highest concentrations of fat, organic acids, sugars, soluble dietary fiber (10.57 ± 0.54; 7.34 ± 0.06; 32.27 ± 1.25; 6.82 ± 0.38 g/100 g DW, respectively) and carotenoids (2.70 ± 0.07 mg/100 g DW).					
31321622	0	63	theme	Fruit	63:67	arg1	Composition					23:33	Chemical Composition	14:33	Chemical Composition	14:33	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	0	63	theme	Fruit	63:67	arg1	Capacity					51:58	Antioxidant Capacity	39:58	Antioxidant Capacity	39:58	Comparison of Chemical Composition and Antioxidant Capacity of Fruit, Flower and Bark of Viburnum opulus.					
31321622	2	64	dep	in	474:475	arg1	vitro					477:481	vitro	477:481	vitro	477:481	Antioxidant capacity was evaluated against ABTS, hydroxyl, peroxyl and superoxide free radicals, and as a reducing power by using in vitro test.					
31321622	5	65	theme	VO	870:871	arg1	compounds					1052:1060	phenolic compounds	1043:1060	the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW)	843:1085	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	65	theme	VO	870:871	arg1	DW					1083:1084	3.98 ± 0.04 g/100 g DW	1063:1084	3.98 ± 0.04 g/100 g DW	1063:1084	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	65	theme	VO	870:871	arg1	parts					857:861	the remaining parts	843:861	the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW)	843:1085	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	3	66	dep	showed	501:506	arg1	tested					588:593	tested	588:593	showed great quantitative differences in the composition of the VO morphological parts tested	501:593	The results showed great quantitative differences in the composition of the VO morphological parts tested.					
31321622	5	67	dep	capacity	897:904	arg1	contents					1023:1030	contents	1023:1030	contents	1023:1030	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	68	theme	phenolic	1043:1050	arg1	compounds					1052:1060	phenolic compounds	1043:1060	the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW)	843:1085	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31321622	5	68	theme	phenolic	1043:1050	arg1	DW					1083:1084	3.98 ± 0.04 g/100 g DW	1063:1084	3.98 ± 0.04 g/100 g DW	1063:1084	Whereas, the bark exceeded the remaining parts of the VO in terms of antioxidant capacity, ash (9.32 ± 0.17 g/100 g DW), total (59.34 ± 0.75 g/100 g DW) and insoluble dietary fiber (58.20 ± 0.73 g/100 g DW) contents as well as phenolic compounds (3.98 ± 0.04 g/100 g DW).					
31187840	8	0	theme	DSS-challenged	1330:1343	arg1	rats					1345:1348	DSS-challenged rats	1330:1348	DSS-challenged rats	1330:1348	populations in DSS-challenged rats.					
31187840	2	1	dep	composition	286:296	arg1	the					273:275	the	273:275	the	273:275	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	2	2	theme	honey	360:364	arg1	composition					286:296	chemical composition	277:296	chemical composition	277:296	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	2	2	theme	honey	360:364	arg1	effects					330:336	gastrointestinal protective effects	302:336	gastrointestinal protective effects	302:336	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	2	3	from	effects	330:336	arg1	PVH					390:392	PVH	390:392	PVH	390:392	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	2	3	from	effects	330:336	arg1	vulgaris					380:387	Prunella vulgaris	371:387	Prunella vulgaris (PVH)	371:393	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	0	4	theme	sulfate	84:90	arg1	colitis					118:124	dextran sulfate sodium-induced ulcerative colitis	76:124	dextran sulfate sodium-induced ulcerative colitis	76:124	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	3	5	theme	samples	524:530	arg1	hydroxymethylfurfural					490:510	hydroxymethylfurfural	490:510	hydroxymethylfurfural	490:510	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	5	theme	samples	524:530	arg1	moisture					428:435	moisture	428:435	moisture	428:435	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	5	theme	samples	524:530	arg1	activity					476:483	diastase activity	467:483	diastase activity	467:483	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	5	theme	samples	524:530	arg1	parameters					416:425	The physicochemical parameters	396:425	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples	396:530	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	5	theme	samples	524:530	arg1	pH					446:447	pH	446:447	pH	446:447	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	5	theme	samples	524:530	arg1	content					458:464	protein content	450:464	protein content	450:464	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	0	6	theme	dextran	76:82	arg1	sulfate					84:90	dextran sulfate	76:90	dextran sulfate sodium-induced ulcerative colitis	76:124	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	6	7	dep	PVH	1014:1016	arg1	g					1021:1021	5 g	1019:1021	5 g per kg b.w., p.o.	1019:1039	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	4	8	theme	flight	767:772	arg1	spectrometry					786:797	flight tandem mass spectrometry	767:797	flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS)	767:827	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	2	9	theme	chemical	277:284	arg1	composition					286:296	chemical composition	277:296	chemical composition	277:296	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	4	10	theme	array	735:739	arg1	detector					741:748	a diode array detector	727:748	a diode array detector	727:748	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	0	11	theme	ulcerative	107:116	arg1	colitis					118:124	dextran sulfate sodium-induced ulcerative colitis	76:124	dextran sulfate sodium-induced ulcerative colitis	76:124	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	4	12	theme	diode	729:733	arg1	detector					741:748	a diode array detector	727:748	a diode array detector	727:748	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	7	13	from	increase	1235:1242	arg1	ratio					1276:1280	the Bacteroidetes/Firmicutes ratio	1247:1280	the Bacteroidetes/Firmicutes ratio	1247:1280	PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp.					
31187840	4	14	theme	spectrometry	786:797	arg1	HPLC-DAD/Q-TOF-MS					810:826	HPLC-DAD/Q-TOF-MS	810:826	HPLC-DAD/Q-TOF-MS	810:826	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	4	14	theme	spectrometry	786:797	arg1	detection					799:807	flight tandem mass spectrometry detection	767:807	flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS)	767:827	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	5	15	theme	potential	864:872	arg1	marker					874:879	a potential marker	862:879	a potential marker for PVH identification	862:902	Rosmarinic acid was found to be a potential marker for PVH identification.					
31187840	5	15	theme	potential	864:872	arg1	acid					841:844	Rosmarinic acid	830:844	Rosmarinic acid	830:844	Rosmarinic acid was found to be a potential marker for PVH identification.					
31187840	0	16	theme	sodium-induced	92:105	arg1	colitis					118:124	dextran sulfate sodium-induced ulcerative colitis	76:124	dextran sulfate sodium-induced ulcerative colitis	76:124	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	0	17	from	plant	32:36	arg1	honey					11:15	Monofloral honey	0:15	Monofloral honey from a medical plant, Prunella Vulgaris,	0:56	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	4	18	theme	phenolic	629:636	arg1	compounds					638:646	Fifteen phenolic compounds	621:646	Fifteen phenolic compounds	621:646	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	3	19	theme	PVH	520:522	arg1	samples					524:530	the PVH samples	516:530	the PVH samples	516:530	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	20	dep	Union	571:575	arg1	regulations					577:587	regulations	577:587	regulations	577:587	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	21	dep	parameters	416:425	arg1	hydroxymethylfurfural					490:510	hydroxymethylfurfural	490:510	hydroxymethylfurfural	490:510	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	21	dep	parameters	416:425	arg1	moisture					428:435	moisture	428:435	moisture	428:435	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	21	dep	parameters	416:425	arg1	sugars					438:443	sugars	438:443	sugars	438:443	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	21	dep	parameters	416:425	arg1	activity					476:483	diastase activity	467:483	diastase activity	467:483	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	21	dep	parameters	416:425	arg1	parameters					416:425	The physicochemical parameters	396:425	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples	396:530	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	21	dep	parameters	416:425	arg1	pH					446:447	pH	446:447	pH	446:447	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	21	dep	parameters	416:425	arg1	content					458:464	protein content	450:464	protein content	450:464	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	9	22	theme	colitis	1465:1471	arg1	prevention					1451:1460	the prevention	1447:1460	the prevention of colitis	1447:1471	The results of this study provide fundamental data on PVH, supporting its future application in the prevention of colitis.					
31187840	0	23	theme	Monofloral	0:9	arg1	honey					11:15	Monofloral honey	0:15	Monofloral honey from a medical plant, Prunella Vulgaris,	0:56	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	6	24	theme	colonic	1107:1113	arg1	changes					1133:1139	colonic histopathological changes	1107:1139	colonic histopathological changes in rats	1107:1147	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	2	25	from	vulgaris	380:387	arg1	composition					286:296	chemical composition	277:296	chemical composition	277:296	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	2	25	from	vulgaris	380:387	arg1	honey					360:364	a novel monofloral honey	341:364	a novel monofloral honey from Prunella vulgaris (PVH)	341:393	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	2	25	from	vulgaris	380:387	arg1	effects					330:336	gastrointestinal protective effects	302:336	gastrointestinal protective effects	302:336	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	2	26	theme	protective	319:328	arg1	effects					330:336	gastrointestinal protective effects	302:336	gastrointestinal protective effects	302:336	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	4	27	theme	tandem	774:779	arg1	spectrometry					786:797	flight tandem mass spectrometry	767:797	flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS)	767:827	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	5	28	theme	PVH	885:887	arg1	identification					889:902	PVH identification	885:902	PVH identification	885:902	Rosmarinic acid was found to be a potential marker for PVH identification.					
31187840	5	29	theme	Rosmarinic	830:839	arg1	marker					874:879	a potential marker	862:879	a potential marker for PVH identification	862:902	Rosmarinic acid was found to be a potential marker for PVH identification.					
31187840	5	29	theme	Rosmarinic	830:839	arg1	acid					841:844	Rosmarinic acid	830:844	Rosmarinic acid	830:844	Rosmarinic acid was found to be a potential marker for PVH identification.					
31187840	2	30	theme	gastrointestinal	302:317	arg1	effects					330:336	gastrointestinal protective effects	302:336	gastrointestinal protective effects	302:336	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	1	31	theme	medicinal	197:205	arg1	plants					207:212	medicinal plants	197:212	medicinal plants	197:212	Honeys produced from medicinal plants hold great promise for human health.					
31187840	6	32	from	changes	1133:1139	arg1	rats					1144:1147	rats	1144:1147	rats	1144:1147	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	6	33	theme	histopathological	1115:1131	arg1	changes					1133:1139	colonic histopathological changes	1107:1139	colonic histopathological changes in rats	1107:1147	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	9	34	theme	future	1425:1430	arg1	application					1432:1442	its future application	1421:1442	its future application in the prevention of colitis	1421:1471	The results of this study provide fundamental data on PVH, supporting its future application in the prevention of colitis.					
31187840	6	35	theme	activity	1078:1085	arg1	index					1087:1091	the disease activity index	1066:1091	the disease activity index	1066:1091	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	7	36	theme	microbiota	1177:1186	arg1	composition					1188:1198	the gut microbiota composition	1169:1198	the gut microbiota composition in the colitic rats	1169:1218	PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp.					
31187840	6	37	theme	colitis	956:962	arg1	model					964:968	a dextran sulfate sodium (DSS)-induced acute colitis model	911:968	a dextran sulfate sodium (DSS)-induced acute colitis model	911:968	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	0	38	theme	gut	141:143	arg1	populations					155:165	gut microbial populations	141:165	gut microbial populations	141:165	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	7	39	theme	gut	1173:1175	arg1	composition					1188:1198	the gut microbiota composition	1169:1198	the gut microbiota composition in the colitic rats	1169:1218	PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp.					
31187840	0	40	theme	medical	24:30	arg1	plant					32:36	a medical plant	22:36	a medical plant	22:36	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	0	40	theme	medical	24:30	arg1	Vulgaris					48:55	Prunella Vulgaris	39:55	Prunella Vulgaris	39:55	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	6	41	theme	acute	950:954	arg1	model					964:968	a dextran sulfate sodium (DSS)-induced acute colitis model	911:968	a dextran sulfate sodium (DSS)-induced acute colitis model	911:968	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	8	42	from	populations	1315:1325	arg1	rats					1345:1348	DSS-challenged rats	1330:1348	DSS-challenged rats	1330:1348	populations in DSS-challenged rats.					
31187840	6	43	theme	PVH	1014:1016	arg1	administration					996:1009	the administration	992:1009	the administration of PVH (5 g per kg b.w., p.o.)	992:1040	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	1	44	theme	great	219:223	arg1	promise					225:231	great promise	219:231	great promise for human health	219:248	Honeys produced from medicinal plants hold great promise for human health.					
31187840	0	45	theme	Prunella	39:46	arg1	plant					32:36	a medical plant	22:36	a medical plant	22:36	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	0	45	theme	Prunella	39:46	arg1	Vulgaris					48:55	Prunella Vulgaris	39:55	Prunella Vulgaris	39:55	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	6	46	theme	-induced	941:948	arg1	model					964:968	a dextran sulfate sodium (DSS)-induced acute colitis model	911:968	a dextran sulfate sodium (DSS)-induced acute colitis model	911:968	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	7	47	from	composition	1188:1198	arg1	rats					1215:1218	the colitic rats	1203:1218	the colitic rats	1203:1218	PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp.					
31187840	2	48	theme	monofloral	349:358	arg1	honey					360:364	a novel monofloral honey	341:364	a novel monofloral honey from Prunella vulgaris (PVH)	341:393	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	3	49	theme	physicochemical	400:414	arg1	hydroxymethylfurfural					490:510	hydroxymethylfurfural	490:510	hydroxymethylfurfural	490:510	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	49	theme	physicochemical	400:414	arg1	moisture					428:435	moisture	428:435	moisture	428:435	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	49	theme	physicochemical	400:414	arg1	activity					476:483	diastase activity	467:483	diastase activity	467:483	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	49	theme	physicochemical	400:414	arg1	parameters					416:425	The physicochemical parameters	396:425	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples	396:530	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	49	theme	physicochemical	400:414	arg1	pH					446:447	pH	446:447	pH	446:447	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	49	theme	physicochemical	400:414	arg1	content					458:464	protein content	450:464	protein content	450:464	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	9	50	theme	study	1371:1375	arg1	results					1355:1361	The results	1351:1361	The results of this study	1351:1375	The results of this study provide fundamental data on PVH, supporting its future application in the prevention of colitis.					
31187840	0	51	theme	microbial	145:153	arg1	populations					155:165	gut microbial populations	141:165	gut microbial populations	141:165	Monofloral honey from a medical plant, Prunella Vulgaris, protected against dextran sulfate sodium-induced ulcerative colitis via modulating gut microbial populations in rats.					
31187840	7	52	theme	Bacteroidetes/Firmicutes	1251:1274	arg1	ratio					1276:1280	the Bacteroidetes/Firmicutes ratio	1247:1280	the Bacteroidetes/Firmicutes ratio	1247:1280	PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp.					
31187840	2	53	theme	novel	343:347	arg1	honey					360:364	a novel monofloral honey	341:364	a novel monofloral honey from Prunella vulgaris (PVH)	341:393	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	7	54	dep	modulated	1159:1167	arg1	restoring					1286:1294	restoring	1286:1294	restoring Lactobacillus spp	1286:1312	PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp.					
31187840	7	54	dep	modulated	1159:1167	arg1	reversing					1221:1229	reversing	1221:1229	reversing the increase in the Bacteroidetes/Firmicutes ratio	1221:1280	PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp.					
31187840	4	55	theme	high-performance	683:698	arg1	chromatography					707:720	high-performance liquid chromatography	683:720	high-performance liquid chromatography	683:720	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	9	56	theme	fundamental	1385:1395	arg1	data					1397:1400	fundamental data	1385:1400	fundamental data on PVH	1385:1407	The results of this study provide fundamental data on PVH, supporting its future application in the prevention of colitis.					
31187840	4	57	theme	mass	781:784	arg1	spectrometry					786:797	flight tandem mass spectrometry	767:797	flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS)	767:827	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	4	58	theme	detection	799:807	arg1	time					759:762	time	759:762	time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS)	759:827	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	3	59	theme	diastase	467:474	arg1	activity					476:483	diastase activity	467:483	diastase activity	467:483	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	59	theme	diastase	467:474	arg1	parameters					416:425	The physicochemical parameters	396:425	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples	396:530	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	2	60	from	composition	286:296	arg1	PVH					390:392	PVH	390:392	PVH	390:392	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	2	60	from	composition	286:296	arg1	vulgaris					380:387	Prunella vulgaris	371:387	Prunella vulgaris (PVH)	371:393	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	9	61	from	data	1397:1400	arg1	PVH					1405:1407	PVH	1405:1407	PVH	1405:1407	The results of this study provide fundamental data on PVH, supporting its future application in the prevention of colitis.					
31187840	7	62	theme	colitic	1207:1213	arg1	rats					1215:1218	the colitic rats	1203:1218	the colitic rats	1203:1218	PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp.					
31187840	6	63	theme	disease	1070:1076	arg1	index					1087:1091	the disease activity index	1066:1091	the disease activity index	1066:1091	Using a dextran sulfate sodium (DSS)-induced acute colitis model, we demonstrated that the administration of PVH (5 g per kg b.w., p.o.) significantly decreased the disease activity index and mitigated colonic histopathological changes in rats.					
31187840	3	64	theme	Chinese	593:599	arg1	Standards					610:618	Chinese National Standards	593:618	Chinese National Standards	593:618	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	65	theme	protein	450:456	arg1	parameters					416:425	The physicochemical parameters	396:425	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples	396:530	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	3	65	theme	protein	450:456	arg1	content					458:464	protein content	450:464	protein content	450:464	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	4	66	theme	liquid	700:705	arg1	chromatography					707:720	high-performance liquid chromatography	683:720	high-performance liquid chromatography	683:720	Fifteen phenolic compounds were identified and quantified via high-performance liquid chromatography with a diode array detector and with time of flight tandem mass spectrometry detection (HPLC-DAD/Q-TOF-MS).					
31187840	7	67	theme	Lactobacillus	1296:1308	arg1	spp					1310:1312	Lactobacillus spp	1296:1312	Lactobacillus spp	1296:1312	PVH also modulated the gut microbiota composition in the colitic rats, reversing the increase in the Bacteroidetes/Firmicutes ratio and restoring Lactobacillus spp.					
31187840	1	68	theme	human	237:241	arg1	health					243:248	human health	237:248	human health	237:248	Honeys produced from medicinal plants hold great promise for human health.					
31187840	3	69	theme	National	601:608	arg1	Standards					610:618	Chinese National Standards	593:618	Chinese National Standards	593:618	The physicochemical parameters (moisture, sugars, pH, protein content, diastase activity, and hydroxymethylfurfural) of the PVH samples met the criteria specified in European Union regulations and Chinese National Standards.					
31187840	9	70	from	application	1432:1442	arg1	prevention					1451:1460	the prevention	1447:1460	the prevention of colitis	1447:1471	The results of this study provide fundamental data on PVH, supporting its future application in the prevention of colitis.					
31187840	2	71	theme	Prunella	371:378	arg1	PVH					390:392	PVH	390:392	PVH	390:392	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31187840	2	71	theme	Prunella	371:378	arg1	vulgaris					380:387	Prunella vulgaris	371:387	Prunella vulgaris (PVH)	371:393	Herein, we determined the chemical composition and gastrointestinal protective effects of a novel monofloral honey from Prunella vulgaris (PVH).					
31320053	4	0	theme	light	973:977	arg1	biowindows					998:1007	the first and second near infrared light (NIR I and NIR II) biowindows	938:1007	the first and second near infrared light (NIR I and NIR II) biowindows	938:1007	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	7	1	theme	NIR	1430:1432	arg1	II					1434:1435	NIR II	1430:1435	NIR II	1430:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	2	2	from	temperature	429:439	arg1	present					448:454	the present	444:454	the present of β-glycerophosphate (β-GP)	444:483	Chitosan (CS) solution was injected into the tumor at room temperature and automatically gelled after warming to body temperature in the present of β-glycerophosphate (β-GP).					
31320053	2	3	theme	room	365:368	arg1	temperature					370:380	room temperature	365:380	room temperature	365:380	Chitosan (CS) solution was injected into the tumor at room temperature and automatically gelled after warming to body temperature in the present of β-glycerophosphate (β-GP).					
31320053	1	4	theme	colon	267:271	arg1	cancer					273:278	safe and high efficient in vivo colon cancer hyperthermia and chemotherapy	235:308	cancer	273:278	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	4	5	theme	infrared	964:971	arg1	light					973:977	the first and second near infrared light	938:977	the first and second near infrared light (NIR I and NIR II) biowindows	938:1007	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	7	6	theme	NIR	1420:1422	arg1	I					1424:1424	NIR I	1420:1424	NIR I	1420:1424	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	5	7	from	hydrogel	1103:1110	arg1	release					1075:1081	the release	1071:1081	the release of the DOX from CMD hydrogel	1071:1110	Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation.					
31320053	5	7	from	hydrogel	1103:1110	arg1	controllable					1116:1127	controllable	1116:1127	controllable	1116:1127	Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation.					
31320053	4	8	theme	low	1027:1029	arg1	concentration					1035:1047	a low MBP concentration	1025:1047	a low MBP concentration (0.5 mg/mL)	1025:1059	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	4	8	theme	low	1027:1029	arg1	0.5 mg/mL					1050:1058	0.5 mg/mL	1050:1058	0.5 mg/mL	1050:1058	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	0	9	theme	cancer	124:129	arg1	chemotherapy					102:113	chemotherapy	102:113	chemotherapy of colon cancer	102:129	Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer.					
31320053	0	9	theme	cancer	124:129	arg1	hyperthermia					85:96	combined hyperthermia	76:96	combined hyperthermia	76:96	Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer.					
31320053	7	10	theme	I	1424:1424	arg1	depths					1410:1415	the different penetration depths	1384:1415	the different penetration depths of NIR I and NIR II	1384:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	6	11	theme	chitosan-based	1213:1226	arg1	hydrogel					1228:1235	the chitosan-based hydrogel	1209:1235	the chitosan-based hydrogel	1209:1235	Moreover, the chitosan-based hydrogel had antibacterial effects.					
31320053	3	12	theme	photothermal	569:580	arg1	nanosheets					612:621	photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets	569:621	photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets	569:621	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	0	13	theme	colon	118:122	arg1	cancer					124:129	colon cancer	118:129	colon cancer	118:129	Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer.					
31320053	3	14	theme	material	582:589	arg1	nanosheets					612:621	photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets	569:621	photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets	569:621	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	2	15	theme	Chitosan	311:318	arg1	solution					325:332	Chitosan (CS) solution	311:332	Chitosan (CS) solution	311:332	Chitosan (CS) solution was injected into the tumor at room temperature and automatically gelled after warming to body temperature in the present of β-glycerophosphate (β-GP).					
31320053	3	16	theme	localized	495:503	arg1	photothermal					511:522	Combined localized tumor photothermal	486:522	Combined localized tumor photothermal	486:522	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	4	17	theme	CS/MBP/DOX	852:861	arg1	hydrogel					869:876	the CS/MBP/DOX (CMD) hydrogel	848:876	the CS/MBP/DOX (CMD) hydrogel	848:876	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	7	18	theme	tumors	1368:1373	arg1	treatment					1355:1363	the high efficient treatment	1336:1363	the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II	1336:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	1	19	theme	injectable	175:184	arg1	hydrogel					186:193	an injectable hydrogel	172:193	an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy	172:308	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	4	20	theme	first	942:946	arg1	light					973:977	the first and second near infrared light	938:977	the first and second near infrared light (NIR I and NIR II) biowindows	938:1007	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	3	21	theme	MBP	723:725	arg1	nanosheets					727:736	MBP nanosheets	723:736	MBP nanosheets	723:736	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	4	22	theme	NIR	980:982	arg1	I					984:984	NIR I	980:984	NIR I	980:984	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	5	23	theme	CMD	1099:1101	arg1	hydrogel					1103:1110	CMD hydrogel	1099:1110	CMD hydrogel	1099:1110	Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation.					
31320053	2	24	theme	β-glycerophosphate	459:476	arg1	present					448:454	the present	444:454	the present of β-glycerophosphate (β-GP)	444:483	Chitosan (CS) solution was injected into the tumor at room temperature and automatically gelled after warming to body temperature in the present of β-glycerophosphate (β-GP).					
31320053	7	25	theme	penetration	1398:1408	arg1	depths					1410:1415	the different penetration depths	1384:1415	the different penetration depths of NIR I and NIR II	1384:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	4	26	theme	CMD	864:866	arg1	hydrogel					869:876	the CS/MBP/DOX (CMD) hydrogel	848:876	the CS/MBP/DOX (CMD) hydrogel	848:876	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	3	27	theme	MBP	607:609	arg1	nanosheets					612:621	photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets	569:621	photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets	569:621	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	6	28	contain	had	1237:1239	arg1	hydrogel					1228:1235	the chitosan-based hydrogel	1209:1235	the chitosan-based hydrogel	1209:1235	Moreover, the chitosan-based hydrogel had antibacterial effects.					
31320053	6	28	contain	had	1237:1239	arg2	effects					1255:1261	antibacterial effects	1241:1261	antibacterial effects	1241:1261	Moreover, the chitosan-based hydrogel had antibacterial effects.					
31320053	7	29	theme	designed	1268:1275	arg1	platform					1323:1330	a platform	1321:1330	a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II	1321:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	7	29	theme	designed	1268:1275	arg1	hydrogel					1287:1294	The designed composite hydrogel	1264:1294	The designed composite hydrogel	1264:1294	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	4	30	theme	NIR	990:992	arg1	II					994:995	NIR II	990:995	NIR II	990:995	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	5	31	theme	gel	1139:1141	arg1	temperature					1143:1153	the gel temperature	1135:1153	the gel temperature	1135:1153	Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation.					
31320053	1	32	theme	temperature-sensitive	200:220	arg1	property					222:229	temperature-sensitive property	200:229	temperature-sensitive property	200:229	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	0	33	theme	temperature-sensitive	26:46	arg1	hydrogel					63:70	injectable temperature-sensitive chitosan-based hydrogel	15:70	injectable temperature-sensitive chitosan-based hydrogel	15:70	Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer.					
31320053	3	34	theme	normal	804:809	arg1	tissues					811:817	damaging normal tissues	795:817	damaging normal tissues	795:817	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	3	35	theme	MoS2/Bi2S3-PEG	591:604	arg1	nanosheets					612:621	photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets	569:621	photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets	569:621	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	0	36	theme	injectable	15:24	arg1	hydrogel					63:70	injectable temperature-sensitive chitosan-based hydrogel	15:70	injectable temperature-sensitive chitosan-based hydrogel	15:70	Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer.					
31320053	4	37	theme	%	922:922	arg1	efficiency					903:912	a photothermal efficiency	888:912	a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows	888:1007	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	1	38	dep	in	259:260	arg1	vivo					262:265	vivo	262:265	vivo	262:265	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	7	39	theme	efficient	1345:1353	arg1	treatment					1355:1363	the high efficient treatment	1336:1363	the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II	1336:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	0	40	theme	hydrogel	63:70	arg1	Preparation					0:10	Preparation	0:10	Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer.	0:130	Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer.					
31320053	4	41	theme	%	933:933	arg1	efficiency					903:912	a photothermal efficiency	888:912	a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows	888:1007	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	1	42	theme	safe	235:238	arg1	cancer					273:278	safe and high efficient in vivo colon cancer hyperthermia and chemotherapy	235:308	cancer	273:278	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	3	43	theme	drug	627:630	arg1	DOX					654:656	DOX	654:656	DOX	654:656	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	3	43	theme	drug	627:630	arg1	doxorubicin					641:651	drug molecule doxorubicin	627:651	drug molecule doxorubicin (DOX)	627:657	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	7	44	theme	composite	1277:1285	arg1	platform					1323:1330	a platform	1321:1330	a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II	1321:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	7	44	theme	composite	1277:1285	arg1	hydrogel					1287:1294	The designed composite hydrogel	1264:1294	The designed composite hydrogel	1264:1294	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	2	45	theme	body	424:427	arg1	temperature					429:439	body temperature	424:439	body temperature in the present of β-glycerophosphate (β-GP)	424:483	Chitosan (CS) solution was injected into the tumor at room temperature and automatically gelled after warming to body temperature in the present of β-glycerophosphate (β-GP).					
31320053	0	46	theme	chitosan-based	48:61	arg1	hydrogel					63:70	injectable temperature-sensitive chitosan-based hydrogel	15:70	injectable temperature-sensitive chitosan-based hydrogel	15:70	Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer.					
31320053	5	47	theme	DOX	1090:1092	arg1	release					1075:1081	the release	1071:1081	the release of the DOX from CMD hydrogel	1071:1110	Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation.					
31320053	5	47	theme	DOX	1090:1092	arg1	controllable					1116:1127	controllable	1116:1127	controllable	1116:1127	Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation.					
31320053	7	48	theme	high	1340:1343	arg1	treatment					1355:1363	the high efficient treatment	1336:1363	the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II	1336:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	3	49	theme	molecule	632:639	arg1	DOX					654:656	DOX	654:656	DOX	654:656	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	3	49	theme	molecule	632:639	arg1	doxorubicin					641:651	drug molecule doxorubicin	627:651	drug molecule doxorubicin (DOX)	627:657	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	6	50	theme	antibacterial	1241:1253	arg1	effects					1255:1261	antibacterial effects	1241:1261	antibacterial effects	1241:1261	Moreover, the chitosan-based hydrogel had antibacterial effects.					
31320053	0	51	theme	combined	76:83	arg1	hyperthermia					85:96	combined hyperthermia	76:96	combined hyperthermia	76:96	Preparation of injectable temperature-sensitive chitosan-based hydrogel for combined hyperthermia and chemotherapy of colon cancer.					
31320053	3	52	theme	damaging	795:802	arg1	tissues					811:817	damaging normal tissues	795:817	damaging normal tissues	795:817	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	4	53	dep	light	973:977	arg1	I					984:984	NIR I	980:984	NIR I	980:984	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	4	53	dep	light	973:977	arg1	II					994:995	NIR II	990:995	NIR II	990:995	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	7	54	theme	different	1388:1396	arg1	depths					1410:1415	the different penetration depths	1384:1415	the different penetration depths of NIR I and NIR II	1384:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	3	55	theme	tumor	505:509	arg1	photothermal					511:522	Combined localized tumor photothermal	486:522	Combined localized tumor photothermal	486:522	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	3	56	theme	blood	773:777	arg1	circulation					779:789	the blood circulation	769:789	the blood circulation	769:789	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	4	57	theme	photothermal	890:901	arg1	efficiency					903:912	a photothermal efficiency	888:912	a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows	888:1007	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	3	58	theme	gel	686:688	arg1	system					690:695	the gel system	682:695	the gel system	682:695	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	7	59	theme	II	1434:1435	arg1	depths					1410:1415	the different penetration depths	1384:1415	the different penetration depths of NIR I and NIR II	1384:1435	The designed composite hydrogel is anticipated to act as a platform for the high efficient treatment of tumors owing to the different penetration depths of NIR I and NIR II.					
31320053	3	60	theme	Combined	486:493	arg1	photothermal					511:522	Combined localized tumor photothermal	486:522	Combined localized tumor photothermal	486:522	Combined localized tumor photothermal and chemotherapy were achieved by dissolving photothermal material MoS2/Bi2S3-PEG (MBP) nanosheets and drug molecule doxorubicin (DOX) into the hydrogel, and the gel system could encapsulate DOX and MBP nanosheets and prevent them from entering the blood circulation and damaging normal tissues and cells.					
31320053	4	61	theme	MBP	1031:1033	arg1	concentration					1035:1047	a low MBP concentration	1025:1047	a low MBP concentration (0.5 mg/mL)	1025:1059	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	4	61	theme	MBP	1031:1033	arg1	0.5 mg/mL					1050:1058	0.5 mg/mL	1050:1058	0.5 mg/mL	1050:1058	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	1	62	with	hydrogel	186:193	arg1	property					222:229	temperature-sensitive property	200:229	temperature-sensitive property	200:229	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	4	63	from	efficiency	903:912	arg1	biowindows					998:1007	the first and second near infrared light (NIR I and NIR II) biowindows	938:1007	the first and second near infrared light (NIR I and NIR II) biowindows	938:1007	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	1	64	theme	study	152:156	arg1	purpose					136:142	The purpose	132:142	The purpose of this study	132:156	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	1	65	theme	efficient	249:257	arg1	cancer					273:278	safe and high efficient in vivo colon cancer hyperthermia and chemotherapy	235:308	cancer	273:278	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	4	66	theme	near	959:962	arg1	light					973:977	the first and second near infrared light	938:977	the first and second near infrared light (NIR I and NIR II) biowindows	938:1007	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	1	67	dep	cancer	273:278	arg1	hyperthermia					280:291	hyperthermia	280:291	hyperthermia	280:291	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	1	68	theme	in	259:260	arg1	cancer					273:278	safe and high efficient in vivo colon cancer hyperthermia and chemotherapy	235:308	cancer	273:278	The purpose of this study was to design an injectable hydrogel with temperature-sensitive property for safe and high efficient in vivo colon cancer hyperthermia and chemotherapy.					
31320053	4	69	theme	second	952:957	arg1	light					973:977	the first and second near infrared light	938:977	the first and second near infrared light (NIR I and NIR II) biowindows	938:1007	More importantly, the CS/MBP/DOX (CMD) hydrogel exhibited a photothermal efficiency of 22.18% and 31.42% in the first and second near infrared light (NIR I and NIR II) biowindows respectively at a low MBP concentration (0.5 mg/mL).					
31320053	5	70	theme	NIR	1176:1178	arg1	irradiation					1186:1196	NIR laser irradiation	1176:1196	NIR laser irradiation	1176:1196	Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation.					
31320053	5	71	theme	laser	1180:1184	arg1	irradiation					1186:1196	NIR laser irradiation	1176:1196	NIR laser irradiation	1176:1196	Besides, the release of the DOX from CMD hydrogel was controllable since the gel temperature could be governed by NIR laser irradiation.					
31069851	3	0	theme	wrinkled	675:682	arg1	tests					684:688	wrinkled tests	675:688	wrinkled tests	675:688	Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests.					
31069851	4	1	theme	MTT	806:808	arg1	assay					810:814	MTT assay	806:814	MTT assay for up to 14 days	806:832	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	8	2	from	use	1630:1632	arg1	applications					1667:1678	various regenerative medicine applications	1637:1678	various regenerative medicine applications	1637:1678	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	8	3	theme	Cur	1556:1558	arg1	delivery					1540:1547	a delivery	1538:1547	a delivery of the Cur and BFP-MSCs	1538:1571	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	8	4	theme	great	1519:1523	arg1	potential					1525:1533	great potential	1519:1533	great potential	1519:1533	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	7	5	theme	positive	1388:1395	arg1	effect					1397:1402	a positive effect	1386:1402	a positive effect on stem cell viability	1386:1425	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	5	6	theme	culture	1023:1029	arg1	plate					1031:1035	the culture plate	1019:1035	the culture plate	1019:1035	Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate.					
31069851	3	7	theme	contact	604:610	arg1	angle					612:616	water contact angle	598:616	water contact angle	598:616	Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests.					
31069851	0	8	theme	biomedical	93:102	arg1	applications					104:115	biomedical applications	93:115	biomedical applications	93:115	Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications.					
31069851	4	9	theme	buccal	721:726	arg1	cells					761:765	the buccal fat pad-derived mesenchymal stem cells	717:765	the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs)	717:776	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	4	9	theme	buccal	721:726	arg1	BFP-MSCs					768:775	BFP-MSCs	768:775	BFP-MSCs	768:775	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	5	10	theme	stem	940:943	arg1	cells					945:949	stem cells	940:949	the stem cells growth	936:956	Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate.					
31069851	7	11	theme	decreasing	1310:1319	arg1	effect					1321:1326	a decreasing effect	1308:1326	a decreasing effect on BFP-MSCs viability in the initial stage	1308:1369	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	1	12	theme	high	260:263	arg1	area					273:276	high surface area	260:276	high surface area	260:276	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
31069851	2	13	theme	fibers	521:526	arg1	inside					500:505	inside	500:505	inside of the CS/PVA fibers	500:526	In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers.					
31069851	8	14	contain	has	1515:1517	arg2	potential					1525:1533	great potential	1519:1533	great potential	1519:1533	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	8	14	contain	has	1515:1517	arg1	scaffold					1506:1513	this nanofibrous scaffold	1489:1513	this nanofibrous scaffold	1489:1513	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	6	15	theme	nanofibrous	1075:1085	arg1	scaffold					1087:1094	Cur loaded nanofibrous scaffold	1064:1094	Cur loaded nanofibrous scaffold	1064:1094	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	1	16	theme	surface	265:271	arg1	area					273:276	high surface area	260:276	high surface area	260:276	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
31069851	2	17	theme	nanofibrous	318:328	arg1	scaffolds					330:338	nanofibrous scaffolds	318:338	nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone	318:425	In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers.					
31069851	2	18	theme	vinyl	374:378	arg1	poly					369:372	poly	369:372	poly(vinyl alcohol) (PVA)	369:393	In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers.					
31069851	2	18	theme	vinyl	374:378	arg1	alcohol					380:386	vinyl alcohol	374:386	vinyl alcohol	374:386	In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers.					
31069851	6	19	theme	loaded	1068:1073	arg1	scaffold					1087:1094	Cur loaded nanofibrous scaffold	1064:1094	Cur loaded nanofibrous scaffold	1064:1094	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	6	20	theme	suitable	1105:1112	arg1	capability					1114:1123	more suitable capability	1100:1123	more suitable capability for water absorption and mechanical properties	1100:1170	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	2	21	theme	electrospinning	440:454	arg1	technique					456:464	a dual electrospinning technique	433:464	a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers	433:526	In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers.					
31069851	3	22	theme	protein	651:657	arg1	adsorption					659:668	protein adsorption	651:668	protein adsorption	651:668	Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests.					
31069851	6	23	theme	Cur	1064:1066	arg1	scaffold					1087:1094	Cur loaded nanofibrous scaffold	1064:1094	Cur loaded nanofibrous scaffold	1064:1094	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	7	24	from	effect	1397:1402	arg1	viability					1417:1425	stem cell viability	1407:1425	stem cell viability	1407:1425	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	8	25	theme	nanofibrous	1494:1504	arg1	scaffold					1506:1513	this nanofibrous scaffold	1489:1513	this nanofibrous scaffold	1489:1513	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	1	26	theme	regenerative	182:193	arg1	medicine					195:202	regenerative medicine	182:202	regenerative medicine	182:202	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
31069851	0	27	theme	Biological	0:9	arg1	behavior					11:18	Biological behavior	0:18	Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol)	0:76	Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications.					
31069851	6	28	theme	mechanical	1150:1159	arg1	properties					1161:1170	mechanical properties	1150:1170	mechanical properties	1150:1170	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	2	29	used	used	313:316	arg2	we					310:311	we	310:311	we	310:311	In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers.					
31069851	7	30	theme	stem	1407:1410	arg1	viability					1417:1425	stem cell viability	1407:1425	stem cell viability	1407:1425	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	8	31	theme	promising	1606:1614	arg1	potential					1616:1624	the promising potential	1602:1624	the promising potential for use in various regenerative medicine applications	1602:1678	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	7	32	theme	long-term	1432:1440	arg1	manner					1442:1447	a long-term manner	1430:1447	a long-term manner	1430:1447	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	7	33	theme	Cur	1281:1283	arg1	profile					1293:1299	Cur release profile	1281:1299	Cur release profile	1281:1299	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	5	34	theme	detailed	913:920	arg1	evaluation					922:931	more detailed evaluation	908:931	more detailed evaluation of the stem cells growth	908:956	Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate.					
31069851	2	35	dep	technique	456:464	arg1	incorporated					487:498	incorporated	487:498	incorporated inside of the CS/PVA fibers	487:526	In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers.					
31069851	7	36	theme	release	1285:1291	arg1	profile					1293:1299	Cur release profile	1281:1299	Cur release profile	1281:1299	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	5	37	theme	Cell	869:872	arg1	assay					880:884	Cell cycle assay	869:884	Cell cycle assay	869:884	Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate.					
31069851	1	38	theme	volume	281:286	arg1	ratio					288:292	volume ratio	281:292	volume ratio	281:292	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
31069851	6	39	theme	stem	1241:1244	arg1	proliferation					1266:1278	proliferation	1266:1278	proliferation	1266:1278	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	6	39	theme	stem	1241:1244	arg1	viability					1252:1260	viability	1252:1260	viability	1252:1260	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	6	39	theme	stem	1241:1244	arg1	cells					1246:1250	the stem cells viability and proliferation	1237:1278	the stem cells viability and proliferation	1237:1278	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	4	40	link	pad-derived	732:742	arg1	cells					761:765	the buccal fat pad-derived mesenchymal stem cells	717:765	the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs)	717:776	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	4	40	link	pad-derived	732:742	arg1	BFP-MSCs					768:775	BFP-MSCs	768:775	BFP-MSCs	768:775	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	5	41	theme	cycle	874:878	arg1	assay					880:884	Cell cycle assay	869:884	Cell cycle assay	869:884	Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate.					
31069851	1	42	theme	drug	208:211	arg1	delivery					213:220	drug delivery	208:220	drug delivery	208:220	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
31069851	6	43	theme	water	1129:1133	arg1	absorption					1135:1144	water absorption	1129:1144	water absorption	1129:1144	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	3	44	theme	water	598:602	arg1	angle					612:616	water contact angle	598:616	water contact angle	598:616	Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests.					
31069851	4	45	theme	cells	761:765	arg1	viability					704:712	viability	704:712	viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs)	704:776	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	7	46	theme	cell	1412:1415	arg1	viability					1417:1425	stem cell viability	1407:1425	stem cell viability	1407:1425	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	0	47	theme	curcumin	27:34	arg1	behavior					11:18	Biological behavior	0:18	Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol)	0:76	Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications.					
31069851	6	48	contain	had	1096:1098	arg2	capability					1114:1123	more suitable capability	1100:1123	more suitable capability for water absorption and mechanical properties	1100:1170	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	6	48	contain	had	1096:1098	arg1	scaffold					1087:1094	Cur loaded nanofibrous scaffold	1064:1094	Cur loaded nanofibrous scaffold	1064:1094	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	3	49	theme	electron	577:584	arg1	microscopy					586:595	scanning electron microscopy	568:595	scanning electron microscopy	568:595	Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests.					
31069851	4	50	theme	stem	756:759	arg1	cells					761:765	the buccal fat pad-derived mesenchymal stem cells	717:765	the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs)	717:776	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	4	50	theme	stem	756:759	arg1	BFP-MSCs					768:775	BFP-MSCs	768:775	BFP-MSCs	768:775	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	6	51	dep	cells	1246:1250	arg1	proliferation					1266:1278	proliferation	1266:1278	proliferation	1266:1278	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	6	51	dep	cells	1246:1250	arg1	viability					1252:1260	viability	1252:1260	viability	1252:1260	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	6	51	dep	cells	1246:1250	arg1	cells					1246:1250	the stem cells viability and proliferation	1237:1278	the stem cells viability and proliferation	1237:1278	Results demonstrated that Cur loaded nanofibrous scaffold had more suitable capability for water absorption and mechanical properties compared with the scaffold without Cur and it could also support the stem cells viability and proliferation.					
31069851	2	52	theme	CS/PVA	514:519	arg1	fibers					521:526	the CS/PVA fibers	510:526	the CS/PVA fibers	510:526	In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers.					
31069851	4	53	dep	14	826:827	arg1	to					823:824	to	823:824	to	823:824	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	0	54	dep	incorporated	36:47	arg1	alcohol					69:75	vinyl alcohol	63:75	vinyl alcohol	63:75	Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications.					
31069851	0	54	dep	incorporated	36:47	arg1	curcumin					27:34	the curcumin	23:34	the curcumin incorporated chitosan/poly(vinyl alcohol)	23:76	Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications.					
31069851	7	55	theme	initial	1357:1363	arg1	stage					1365:1369	the initial stage	1353:1369	the initial stage	1353:1369	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	1	56	theme	Electrospun	118:128	arg1	scaffolds					140:148	Electrospun composite scaffolds	118:148	Electrospun composite scaffolds	118:148	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
31069851	8	57	dep	Cur	1556:1558	arg1	the					1552:1554	the	1552:1554	the	1552:1554	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	5	58	theme	growth	951:956	arg1	evaluation					922:931	more detailed evaluation	908:931	more detailed evaluation of the stem cells growth	908:956	Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate.					
31069851	5	59	theme	cells	945:949	arg1	growth					951:956	the stem cells growth	936:956	the stem cells growth	936:956	Cell cycle assay was also performed to more detailed evaluation of the stem cells growth when grown on scaffolds (with and without Cur) compared with the culture plate.					
31069851	8	60	theme	regenerative	1645:1656	arg1	applications					1667:1678	various regenerative medicine applications	1637:1678	various regenerative medicine applications	1637:1678	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	1	61	theme	composite	130:138	arg1	scaffolds					140:148	Electrospun composite scaffolds	118:148	Electrospun composite scaffolds	118:148	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
31069851	3	62	theme	tensile	619:625	arg1	measurement					627:637	tensile measurement	619:637	tensile measurement	619:637	Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests.					
31069851	4	63	theme	fat	728:730	arg1	cells					761:765	the buccal fat pad-derived mesenchymal stem cells	717:765	the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs)	717:776	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	4	63	theme	fat	728:730	arg1	BFP-MSCs					768:775	BFP-MSCs	768:775	BFP-MSCs	768:775	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	7	64	theme	BFP-MSCs	1331:1338	arg1	viability					1340:1348	BFP-MSCs viability	1331:1348	BFP-MSCs viability	1331:1348	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	8	65	theme	various	1637:1643	arg1	applications					1667:1678	various regenerative medicine applications	1637:1678	various regenerative medicine applications	1637:1678	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	1	66	dep	structure	246:254	arg1	the					230:232	the	230:232	the	230:232	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
31069851	3	67	theme	scanning	568:575	arg1	microscopy					586:595	scanning electron microscopy	568:595	scanning electron microscopy	568:595	Scaffolds were fully characterized via scanning electron microscopy, water contact angle, tensile measurement, hydration, protein adsorption, and wrinkled tests.					
31069851	7	68	from	effect	1321:1326	arg1	viability					1340:1348	BFP-MSCs viability	1331:1348	BFP-MSCs viability	1331:1348	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	7	68	from	effect	1321:1326	arg1	stage					1365:1369	the initial stage	1353:1369	the initial stage	1353:1369	Cur release profile showed a decreasing effect on BFP-MSCs viability in the initial stage, but it showed a positive effect on stem cell viability in a long-term manner.					
31069851	2	69	theme	dual	435:438	arg1	technique					456:464	a dual electrospinning technique	433:464	a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers	433:526	In this study, we used nanofibrous scaffolds fabricated by chitosan (CS), poly(vinyl alcohol) (PVA), carbopol, and polycaprolactone using a dual electrospinning technique while curcumin (Cur) incorporated inside of the CS/PVA fibers.					
31069851	8	70	theme	BFP-MSCs	1564:1571	arg1	delivery					1540:1547	a delivery	1538:1547	a delivery of the Cur and BFP-MSCs	1538:1571	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	4	71	theme	mesenchymal	744:754	arg1	cells					761:765	the buccal fat pad-derived mesenchymal stem cells	717:765	the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs)	717:776	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	4	71	theme	mesenchymal	744:754	arg1	BFP-MSCs					768:775	BFP-MSCs	768:775	BFP-MSCs	768:775	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	8	72	theme	medicine	1658:1665	arg1	applications					1667:1678	various regenerative medicine applications	1637:1678	various regenerative medicine applications	1637:1678	In general, the results indicated that this nanofibrous scaffold has great potential as a delivery of the Cur and BFP-MSCs simultaneously, and so holds the promising potential for use in various regenerative medicine applications.					
31069851	1	73	theme	high	155:158	arg1	ability					160:166	high ability	155:166	high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio	155:292	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
31069851	0	74	theme	vinyl	63:67	arg1	alcohol					69:75	vinyl alcohol	63:75	vinyl alcohol	63:75	Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications.					
31069851	0	74	theme	vinyl	63:67	arg1	curcumin					27:34	the curcumin	23:34	the curcumin incorporated chitosan/poly(vinyl alcohol)	23:76	Biological behavior of the curcumin incorporated chitosan/poly(vinyl alcohol) nanofibers for biomedical applications.					
31069851	4	75	theme	pad-derived	732:742	arg1	cells					761:765	the buccal fat pad-derived mesenchymal stem cells	717:765	the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs)	717:776	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	4	75	theme	pad-derived	732:742	arg1	BFP-MSCs					768:775	BFP-MSCs	768:775	BFP-MSCs	768:775	Furthermore, viability of the buccal fat pad-derived mesenchymal stem cells (BFP-MSCs) was also investigated using MTT assay for up to 14 days while cultured on these scaffolds.					
31069851	1	76	theme	nanofibrous	234:244	arg1	structure					246:254	nanofibrous structure	234:254	nanofibrous structure	234:254	Electrospun composite scaffolds show high ability to be used in regenerative medicine and drug delivery, due to the nanofibrous structure and high surface area to volume ratio.					
30888350	15	0	theme	different	2126:2134	arg1	rates					2136:2140	different rates	2126:2140	different rates	2126:2140	The extrudates with similar composition may be digested at different rates depending on their structural attributes.					
30888350	0	1	theme	fibre	133:137	arg1	extrudates					143:152	high fibre rye extrudates	128:152	high fibre rye extrudates	128:152	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	14	2	theme	extrudates	1937:1946	arg1	attributes					1919:1928	the structural attributes	1904:1928	the structural attributes of the extrudates	1904:1946	The results demonstrate that the structural attributes of the extrudates, rather than the core composition, dictate the breakdown pattern during mastication and in vitro starch digestibility.					
30888350	6	3	theme	bran	1077:1080	arg1	weight					1087:1092	100 g bran; dry weight	1071:1092	100 g bran; dry weight	1071:1092	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	7	4	theme	instrumental	1205:1216	arg1	texture					1218:1224	instrumental texture	1205:1224	instrumental texture	1205:1224	The compositionally similar extrudates (NBE vs. FBE) varied in both structure and instrumental texture: FBE were less dense, less hard and crispier than NBE.					
30888350	4	5	theme	endosperm	542:550	arg1	flour					556:560	endosperm rye flour	542:560	endosperm rye flour (EF)	542:565	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	5	theme	endosperm	542:550	arg1	EF					563:564	EF	563:564	EF	563:564	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	12	6	theme	FBE	1752:1754	arg1	samples					1762:1768	FBE bolus samples	1752:1768	FBE bolus samples	1752:1768	There was a large number of smaller particles in both NBE and FBE bolus samples.					
30888350	13	7	dep	in	1844:1845	arg1	vitro					1847:1851	vitro	1847:1851	vitro	1847:1851	The fragile structure of FBE, and its lower bolus viscosity, led to high in vitro starch digestibility.					
30888350	14	8	theme	structural	1908:1917	arg1	attributes					1919:1928	the structural attributes	1904:1928	the structural attributes of the extrudates	1904:1946	The results demonstrate that the structural attributes of the extrudates, rather than the core composition, dictate the breakdown pattern during mastication and in vitro starch digestibility.					
30888350	2	9	theme	sensory	340:346	arg1	properties					348:357	sensory properties	340:357	sensory properties	340:357	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	12	10	theme	smaller	1718:1724	arg1	particles					1726:1734	smaller particles	1718:1734	smaller particles	1718:1734	There was a large number of smaller particles in both NBE and FBE bolus samples.					
30888350	16	11	theme	vitro	2211:2215	arg1	digestibility					2224:2236	vitro starch digestibility	2211:2236	vitro starch digestibility	2211:2236	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	2	12	theme	native	284:289	arg1	bran					308:311	native or fermented rye bran	284:311	native or fermented rye bran	284:311	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	1	13	theme	product	165:171	arg1	category					173:180	The snack product category	155:180	The snack product category	155:180	The snack product category is lacking palatable, high dietary fiber containing products.					
30888350	6	14	dep	in	1095:1096	arg1	situ					1098:1101	situ	1098:1101	situ	1098:1101	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	9	15	theme	subtle	1434:1439	arg1	differences					1452:1462	subtle structural differences	1434:1462	subtle structural differences	1434:1462	There were also subtle structural differences FBE being somewhat denser than EFE.					
30888350	5	16	theme	different	895:903	arg1	structure					905:913	different structure	895:913	different structure	895:913	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	6	17	theme	%	1060:1060	arg1	production					1111:1120	3% (3 g per 100 g bran; dry weight) in situ dextran production	1059:1120	3% (3 g per 100 g bran; dry weight) in situ dextran production	1059:1120	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	13	18	theme	bolus	1815:1819	arg1	viscosity					1821:1829	its lower bolus viscosity	1805:1829	its lower bolus viscosity	1805:1829	The fragile structure of FBE, and its lower bolus viscosity, led to high in vitro starch digestibility.					
30888350	13	19	theme	in	1844:1845	arg1	digestibility					1860:1872	high in vitro starch digestibility	1839:1872	high in vitro starch digestibility	1839:1872	The fragile structure of FBE, and its lower bolus viscosity, led to high in vitro starch digestibility.					
30888350	7	20	dep	extrudates	1151:1160	arg1	NBE					1163:1165	NBE	1163:1165	NBE	1163:1165	The compositionally similar extrudates (NBE vs. FBE) varied in both structure and instrumental texture: FBE were less dense, less hard and crispier than NBE.					
30888350	7	20	dep	extrudates	1151:1160	arg1	FBE					1171:1173	FBE	1171:1173	FBE	1171:1173	The compositionally similar extrudates (NBE vs. FBE) varied in both structure and instrumental texture: FBE were less dense, less hard and crispier than NBE.					
30888350	0	21	theme	high	128:131	arg1	extrudates					143:152	high fibre rye extrudates	128:152	high fibre rye extrudates	128:152	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	5	22	theme	similar	866:872	arg1	composition					879:889	similar core composition	866:889	similar core composition	866:889	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	15	23	theme	similar	2087:2093	arg1	composition					2095:2105	similar composition	2087:2105	similar composition	2087:2105	The extrudates with similar composition may be digested at different rates depending on their structural attributes.					
30888350	7	24	theme	similar	1143:1149	arg1	extrudates					1151:1160	The compositionally similar extrudates	1123:1160	The compositionally similar extrudates (NBE vs. FBE)	1123:1174	The compositionally similar extrudates (NBE vs. FBE) varied in both structure and instrumental texture: FBE were less dense, less hard and crispier than NBE.					
30888350	6	25	theme	dry	1083:1085	arg1	weight					1087:1092	100 g bran; dry weight	1071:1092	100 g bran; dry weight	1071:1092	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	8	26	dep	composition	1311:1321	arg1	FBE					1332:1334	FBE	1332:1334	FBE	1332:1334	The extrudates with different composition (EFE vs. FBE) varied regarding instrumental texture: FBE were less hard and crispier than EFE.					
30888350	8	26	dep	composition	1311:1321	arg1	EFE					1324:1326	EFE	1324:1326	EFE	1324:1326	The extrudates with different composition (EFE vs. FBE) varied regarding instrumental texture: FBE were less hard and crispier than EFE.					
30888350	10	27	theme	FBE	1573:1575	arg1	textures					1553:1560	textures	1553:1560	textures of EFE and FBE	1553:1575	NBE and FBE differed regarding sensory texture while textures of EFE and FBE were perceived similar.					
30888350	4	28	dep	%	636:636	arg1	FBE					658:660	FBE	658:660	FBE	658:660	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	28	dep	%	636:636	arg1	bran					652:655	fermented rye bran	638:655	40% fermented rye bran (FBE)	634:661	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	29	theme	native	609:614	arg1	NBE					626:628	NBE	626:628	NBE	626:628	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	29	theme	native	609:614	arg1	bran					620:623	native rye bran	609:623	either 40% native rye bran (NBE)	598:629	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	0	30	theme	breakdown	43:51	arg1	pattern					53:59	the breakdown pattern	39:59	the breakdown pattern during mastication	39:78	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	15	31	theme	structural	2161:2170	arg1	attributes					2172:2181	their structural attributes	2155:2181	their structural attributes	2155:2181	The extrudates with similar composition may be digested at different rates depending on their structural attributes.					
30888350	10	32	theme	EFE	1565:1567	arg1	textures					1553:1560	textures	1553:1560	textures of EFE and FBE	1553:1575	NBE and FBE differed regarding sensory texture while textures of EFE and FBE were perceived similar.					
30888350	16	33	theme	improved	2282:2289	arg1	properties					2299:2308	improved sensory properties	2282:2308	improved sensory properties	2282:2308	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	0	34	theme	rye	139:141	arg1	extrudates					143:152	high fibre rye extrudates	128:152	high fibre rye extrudates	128:152	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	6	35	dep	%	1060:1060	arg1	g					1065:1065	3 g	1063:1065	3 g per 100 g bran; dry weight	1063:1092	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	2	36	dep	texture	328:334	arg1	the					324:326	the	324:326	the	324:326	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	5	37	theme	bran	922:925	arg1	fermentation					927:938	bran fermentation	922:938	bran fermentation	922:938	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	16	38	theme	important	2315:2323	arg1	determinants					2325:2336	important determinants	2315:2336	important determinants underlying eating quality	2315:2362	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	2	39	theme	extrudates	388:397	arg1	texture					328:334	texture	328:334	texture	328:334	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	2	39	theme	extrudates	388:397	arg1	properties					348:357	sensory properties	340:357	sensory properties	340:357	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	14	40	theme	breakdown	1995:2003	arg1	pattern					2005:2011	the breakdown pattern	1991:2011	the breakdown pattern during mastication and in vitro starch digestibility	1991:2064	The results demonstrate that the structural attributes of the extrudates, rather than the core composition, dictate the breakdown pattern during mastication and in vitro starch digestibility.					
30888350	2	41	theme	rye	372:374	arg1	extrudates					388:397	endosperm rye flour based extrudates	362:397	endosperm rye flour based extrudates	362:397	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	0	42	theme	texture	28:34	arg1	effect					4:9	The effect	0:9	The effect of structure and texture on the breakdown pattern during mastication	0:78	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	4	43	dep	%	607:607	arg1	NBE					626:628	NBE	626:628	NBE	626:628	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	43	dep	%	607:607	arg1	bran					620:623	native rye bran	609:623	either 40% native rye bran (NBE)	598:629	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	44	theme	%	636:636	arg1	addition					586:593	addition	586:593	addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE)	586:661	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	11	45	theme	products	1641:1648	arg1	properties					1613:1622	Mastication properties	1601:1622	Mastication properties of the different products	1601:1648	Mastication properties of the different products did not exhibit remarkable differences.					
30888350	8	46	theme	different	1301:1309	arg1	composition					1311:1321	different composition	1301:1321	different composition (EFE vs. FBE)	1301:1335	The extrudates with different composition (EFE vs. FBE) varied regarding instrumental texture: FBE were less hard and crispier than EFE.					
30888350	4	47	theme	rye	648:650	arg1	FBE					658:660	FBE	658:660	FBE	658:660	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	47	theme	rye	648:650	arg1	bran					652:655	fermented rye bran	638:655	40% fermented rye bran (FBE)	634:661	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	6	48	theme	strain	1020:1025	arg1	confusa					1037:1043	strain Weissella confusa	1020:1043	strain Weissella confusa	1020:1043	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	4	49	theme	high	511:514	arg1	extrudates					522:531	Three high fiber extrudates	505:531	Three high fiber extrudates based on endosperm rye flour (EF)	505:565	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	2	50	theme	based	382:386	arg1	extrudates					388:397	endosperm rye flour based extrudates	362:397	endosperm rye flour based extrudates	362:397	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	11	51	theme	remarkable	1666:1675	arg1	differences					1677:1687	remarkable differences	1666:1687	remarkable differences	1666:1687	Mastication properties of the different products did not exhibit remarkable differences.					
30888350	16	52	theme	high	2243:2246	arg1	content					2251:2257	its high DF content	2239:2257	its high DF content	2239:2257	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	6	53	theme	bran	961:964	arg1	fermentation					945:956	The fermentation	941:956	The fermentation of bran	941:964	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	5	54	contain	had	862:864	arg2	structure					905:913	different structure	895:913	different structure	895:913	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	5	54	contain	had	862:864	arg1	pair					843:846	the second pair	832:846	the second pair (NBE vs. FBE)	832:860	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	5	54	contain	had	862:864	arg2	composition					879:889	similar core composition	866:889	similar core composition	866:889	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	1	55	contain	containing	223:232	arg2	products					234:241	products	234:241	products	234:241	The snack product category is lacking palatable, high dietary fiber containing products.					
30888350	1	55	contain	containing	223:232	arg1	fiber					217:221	high dietary fiber	204:221	high dietary fiber containing products	204:241	The snack product category is lacking palatable, high dietary fiber containing products.					
30888350	1	55	contain	containing	223:232	arg1	palatable					193:201	palatable	193:201	palatable	193:201	The snack product category is lacking palatable, high dietary fiber containing products.					
30888350	2	56	theme	rye	304:306	arg1	bran					308:311	native or fermented rye bran	284:311	native or fermented rye bran	284:311	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	16	57	theme	palatable	2260:2268	arg1	texture					2270:2276	palatable texture	2260:2276	palatable texture	2260:2276	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	14	58	dep	in	2036:2037	arg1	vitro					2039:2043	vitro	2039:2043	vitro	2039:2043	The results demonstrate that the structural attributes of the extrudates, rather than the core composition, dictate the breakdown pattern during mastication and in vitro starch digestibility.					
30888350	4	59	theme	rye	552:554	arg1	flour					556:560	endosperm rye flour	542:560	endosperm rye flour (EF)	542:565	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	59	theme	rye	552:554	arg1	EF					563:564	EF	563:564	EF	563:564	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	3	60	theme	bolus	429:433	arg1	properties					435:444	bolus properties	429:444	bolus properties	429:444	In addition, mastication and bolus properties (n = 26), and in vitro starch digestibility were assessed.					
30888350	3	61	theme	in	460:461	arg1	digestibility					476:488	in vitro starch digestibility	460:488	in vitro starch digestibility	460:488	In addition, mastication and bolus properties (n = 26), and in vitro starch digestibility were assessed.					
30888350	1	62	theme	dietary	209:215	arg1	fiber					217:221	high dietary fiber	204:221	high dietary fiber containing products	204:241	The snack product category is lacking palatable, high dietary fiber containing products.					
30888350	1	62	theme	dietary	209:215	arg1	palatable					193:201	palatable	193:201	palatable	193:201	The snack product category is lacking palatable, high dietary fiber containing products.					
30888350	12	63	theme	bolus	1756:1760	arg1	samples					1762:1768	FBE bolus samples	1752:1768	FBE bolus samples	1752:1768	There was a large number of smaller particles in both NBE and FBE bolus samples.					
30888350	16	64	theme	snack	2413:2417	arg1	category					2424:2431	snack food category	2413:2431	snack food category	2413:2431	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	13	65	theme	starch	1853:1858	arg1	digestibility					1860:1872	high in vitro starch digestibility	1839:1872	high in vitro starch digestibility	1839:1872	The fragile structure of FBE, and its lower bolus viscosity, led to high in vitro starch digestibility.					
30888350	6	66	theme	g	1075:1075	arg1	weight					1087:1092	100 g bran; dry weight	1071:1092	100 g bran; dry weight	1071:1092	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	4	67	theme	extrudates	717:726	arg1	pairs					708:712	two pairs	704:712	two pairs of extrudates	704:726	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	3	68	theme	starch	469:474	arg1	digestibility					476:488	in vitro starch digestibility	460:488	in vitro starch digestibility	460:488	In addition, mastication and bolus properties (n = 26), and in vitro starch digestibility were assessed.					
30888350	16	69	theme	starch	2217:2222	arg1	digestibility					2224:2236	vitro starch digestibility	2211:2236	vitro starch digestibility	2211:2236	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	10	70	theme	sensory	1531:1537	arg1	texture					1539:1545	sensory texture	1531:1545	sensory texture	1531:1545	NBE and FBE differed regarding sensory texture while textures of EFE and FBE were perceived similar.					
30888350	1	71	theme	snack	159:163	arg1	category					173:180	The snack product category	155:180	The snack product category	155:180	The snack product category is lacking palatable, high dietary fiber containing products.					
30888350	16	72	theme	eating	2349:2354	arg1	quality					2356:2362	eating quality	2349:2362	eating quality	2349:2362	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	0	73	theme	starch	104:109	arg1	digestibility					111:123	in vitro starch digestibility	95:123	in vitro starch digestibility of high fibre rye extrudates	95:152	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	12	74	theme	particles	1726:1734	arg1	number					1708:1713	a large number	1700:1713	a large number of smaller particles	1700:1734	There was a large number of smaller particles in both NBE and FBE bolus samples.					
30888350	9	75	theme	structural	1441:1450	arg1	differences					1452:1462	subtle structural differences	1434:1462	subtle structural differences	1434:1462	There were also subtle structural differences FBE being somewhat denser than EFE.					
30888350	2	76	theme	bran	308:311	arg1	addition					272:279	the addition	268:279	the addition of native or fermented rye bran	268:311	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	6	77	theme	dextran	1103:1109	arg1	production					1111:1120	3% (3 g per 100 g bran; dry weight) in situ dextran production	1059:1120	3% (3 g per 100 g bran; dry weight) in situ dextran production	1059:1120	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	13	78	theme	high	1839:1842	arg1	digestibility					1860:1872	high in vitro starch digestibility	1839:1872	high in vitro starch digestibility	1839:1872	The fragile structure of FBE, and its lower bolus viscosity, led to high in vitro starch digestibility.					
30888350	6	79	theme	3	1059:1059	arg1	%					1060:1060	%	1060:1060	%	1060:1060	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	5	80	dep	pair	843:846	arg1	FBE					857:859	FBE	857:859	FBE	857:859	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	5	80	dep	pair	843:846	arg1	NBE					849:851	NBE	849:851	NBE	849:851	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	6	81	theme	in	1095:1096	arg1	production					1111:1120	3% (3 g per 100 g bran; dry weight) in situ dextran production	1059:1120	3% (3 g per 100 g bran; dry weight) in situ dextran production	1059:1120	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	12	82	theme	large	1702:1706	arg1	number					1708:1713	a large number	1700:1713	a large number of smaller particles	1700:1734	There was a large number of smaller particles in both NBE and FBE bolus samples.					
30888350	5	83	contain	had	752:754	arg1	EFE					740:742	EFE	740:742	EFE	740:742	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	5	83	contain	had	752:754	arg2	composition					766:776	different composition	756:776	different composition	756:776	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	5	83	contain	had	752:754	arg1	FBE					748:750	FBE	748:750	FBE	748:750	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	0	84	theme	extrudates	143:152	arg1	digestibility					111:123	in vitro starch digestibility	95:123	in vitro starch digestibility of high fibre rye extrudates	95:152	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	5	85	theme	core	874:877	arg1	composition					879:889	similar core composition	866:889	similar core composition	866:889	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	5	86	theme	different	756:764	arg1	composition					766:776	different composition	756:776	different composition	756:776	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	14	87	theme	starch	2045:2050	arg1	digestibility					2052:2064	in vitro starch digestibility	2036:2064	in vitro starch digestibility	2036:2064	The results demonstrate that the structural attributes of the extrudates, rather than the core composition, dictate the breakdown pattern during mastication and in vitro starch digestibility.					
30888350	16	88	theme	sensory	2291:2297	arg1	properties					2299:2308	improved sensory properties	2282:2308	improved sensory properties	2282:2308	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	6	89	theme	Weissella	1027:1035	arg1	confusa					1037:1043	strain Weissella confusa	1020:1043	strain Weissella confusa	1020:1043	The fermentation of bran was performed using exopolysaccharide (EPS)-producing strain Weissella confusa, which led to 3% (3 g per 100 g bran; dry weight) in situ dextran production.					
30888350	4	90	theme	%	607:607	arg1	addition					586:593	addition	586:593	addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE)	586:661	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	14	91	theme	in	2036:2037	arg1	digestibility					2052:2064	in vitro starch digestibility	2036:2064	in vitro starch digestibility	2036:2064	The results demonstrate that the structural attributes of the extrudates, rather than the core composition, dictate the breakdown pattern during mastication and in vitro starch digestibility.					
30888350	13	92	theme	fragile	1775:1781	arg1	structure					1783:1791	The fragile structure	1771:1791	The fragile structure of FBE	1771:1798	The fragile structure of FBE, and its lower bolus viscosity, led to high in vitro starch digestibility.					
30888350	0	93	from	effect	4:9	arg1	pattern					53:59	the breakdown pattern	39:59	the breakdown pattern during mastication	39:78	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	4	94	theme	rye	616:618	arg1	NBE					626:628	NBE	626:628	NBE	626:628	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	94	theme	rye	616:618	arg1	bran					620:623	native rye bran	609:623	either 40% native rye bran (NBE)	598:629	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	13	95	theme	lower	1809:1813	arg1	viscosity					1821:1829	its lower bolus viscosity	1805:1829	its lower bolus viscosity	1805:1829	The fragile structure of FBE, and its lower bolus viscosity, led to high in vitro starch digestibility.					
30888350	0	96	dep	in	95:96	arg1	vitro					98:102	vitro	98:102	vitro	98:102	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	2	97	theme	fermented	294:302	arg1	bran					308:311	native or fermented rye bran	284:311	native or fermented rye bran	284:311	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	0	98	theme	in	95:96	arg1	digestibility					111:123	in vitro starch digestibility	95:123	in vitro starch digestibility of high fibre rye extrudates	95:152	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	2	99	theme	endosperm	362:370	arg1	extrudates					388:397	endosperm rye flour based extrudates	362:397	endosperm rye flour based extrudates	362:397	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	13	100	theme	FBE	1796:1798	arg1	structure					1783:1791	The fragile structure	1771:1791	The fragile structure of FBE	1771:1798	The fragile structure of FBE, and its lower bolus viscosity, led to high in vitro starch digestibility.					
30888350	13	100	theme	FBE	1796:1798	arg1	viscosity					1821:1829	its lower bolus viscosity	1805:1829	its lower bolus viscosity	1805:1829	The fragile structure of FBE, and its lower bolus viscosity, led to high in vitro starch digestibility.					
30888350	0	101	theme	structure	14:22	arg1	effect					4:9	The effect	0:9	The effect of structure and texture on the breakdown pattern during mastication	0:78	The effect of structure and texture on the breakdown pattern during mastication and impacts on in vitro starch digestibility of high fibre rye extrudates.					
30888350	3	102	dep	in	460:461	arg1	vitro					463:467	vitro	463:467	vitro	463:467	In addition, mastication and bolus properties (n = 26), and in vitro starch digestibility were assessed.					
30888350	4	103	theme	fiber	516:520	arg1	extrudates					522:531	Three high fiber extrudates	505:531	Three high fiber extrudates based on endosperm rye flour (EF)	505:565	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	104	theme	fermented	638:646	arg1	FBE					658:660	FBE	658:660	FBE	658:660	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	104	theme	fermented	638:646	arg1	bran					652:655	fermented rye bran	638:655	40% fermented rye bran (FBE)	634:661	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	14	105	theme	core	1965:1968	arg1	composition					1970:1980	the core composition	1961:1980	the core composition	1961:1980	The results demonstrate that the structural attributes of the extrudates, rather than the core composition, dictate the breakdown pattern during mastication and in vitro starch digestibility.					
30888350	8	106	with	extrudates	1285:1294	arg1	composition					1311:1321	different composition	1301:1321	different composition (EFE vs. FBE)	1301:1335	The extrudates with different composition (EFE vs. FBE) varied regarding instrumental texture: FBE were less hard and crispier than EFE.					
30888350	9	107	dep	were	1424:1427	arg1	denser					1483:1488	denser	1483:1488	denser	1483:1488	There were also subtle structural differences FBE being somewhat denser than EFE.					
30888350	15	108	with	extrudates	2071:2080	arg1	composition					2095:2105	similar composition	2087:2105	similar composition	2087:2105	The extrudates with similar composition may be digested at different rates depending on their structural attributes.					
30888350	4	109	theme	added	676:680	arg1	bran					682:685	no added bran	673:685	no added bran (EFE)	673:691	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	4	109	theme	added	676:680	arg1	EFE					688:690	EFE	688:690	EFE	688:690	Three high fiber extrudates based on endosperm rye flour (EF) were produced with addition of either 40% native rye bran (NBE) or 40% fermented rye bran (FBE), and with no added bran (EFE) to achieve two pairs of extrudates to compare.					
30888350	2	110	theme	flour	376:380	arg1	extrudates					388:397	endosperm rye flour based extrudates	362:397	endosperm rye flour based extrudates	362:397	This study explored how the addition of native or fermented rye bran influences the texture and sensory properties of endosperm rye flour based extrudates.					
30888350	16	111	theme	promising	2392:2400	arg1	product					2402:2408	a promising product	2390:2408	a promising product to snack food category	2390:2431	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	16	111	theme	promising	2392:2400	arg1	it					2378:2379	it	2378:2379	it	2378:2379	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	11	112	theme	Mastication	1601:1611	arg1	properties					1613:1622	Mastication properties	1601:1622	Mastication properties of the different products	1601:1648	Mastication properties of the different products did not exhibit remarkable differences.					
30888350	16	113	theme	DF	2248:2249	arg1	content					2251:2257	its high DF content	2239:2257	its high DF content	2239:2257	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	5	114	theme	second	836:841	arg1	pair					843:846	the second pair	832:846	the second pair (NBE vs. FBE)	832:860	EFE and FBE had different composition but resembled each other regarding macrostructure and the second pair (NBE vs. FBE) had similar core composition but different structure due to bran fermentation.					
30888350	8	115	theme	instrumental	1354:1365	arg1	texture					1367:1373	instrumental texture	1354:1373	instrumental texture	1354:1373	The extrudates with different composition (EFE vs. FBE) varied regarding instrumental texture: FBE were less hard and crispier than EFE.					
30888350	3	116	theme	=	449:449	arg1	n					447:447	n = 26	447:452	n = 26	447:452	In addition, mastication and bolus properties (n = 26), and in vitro starch digestibility were assessed.					
30888350	3	116	theme	=	449:449	arg1	mastication					413:423	mastication	413:423	mastication	413:423	In addition, mastication and bolus properties (n = 26), and in vitro starch digestibility were assessed.					
30888350	1	117	theme	high	204:207	arg1	fiber					217:221	high dietary fiber	204:221	high dietary fiber containing products	204:241	The snack product category is lacking palatable, high dietary fiber containing products.					
30888350	1	117	theme	high	204:207	arg1	palatable					193:201	palatable	193:201	palatable	193:201	The snack product category is lacking palatable, high dietary fiber containing products.					
30888350	16	118	theme	food	2419:2422	arg1	category					2424:2431	snack food category	2413:2431	snack food category	2413:2431	Although FBE had higher in vitro starch digestibility, its high DF content, palatable texture and improved sensory properties were important determinants underlying eating quality and therefore it could be a promising product to snack food category.					
30888350	11	119	theme	different	1631:1639	arg1	products					1641:1648	the different products	1627:1648	the different products	1627:1648	Mastication properties of the different products did not exhibit remarkable differences.					
31178181	2	0	theme	traits	429:434	arg1	data					404:407	phenotypic data	393:407	phenotypic data of milk composition traits	393:434	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	2	0	theme	traits	429:434	arg1	data					472:475	genotypic data	462:475	genotypic data comprising 626,777 SNP	462:498	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	2	0	theme	traits	429:434	arg1	spectra					449:455	related MIR spectra	437:455	related MIR spectra	437:455	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	6	1	theme	SNP	1128:1130	arg1	QTL					1151:1153	SNP tracking different QTL	1128:1153	SNP tracking different QTL	1128:1153	Because SNP tracking different QTL tend to have different patterns of effect, it was possible to distinguish closely linked QTL.					
31178181	3	2	theme	acid	616:619	arg1	concentrations					621:634	fatty acid concentrations	610:634	fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS)	610:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	7	3	theme	study	1278:1282	arg1	results					1262:1268	the results	1258:1268	the results of this study	1258:1282	Overall, the results of this study suggest that using MIR data through either GWAS or PLS analysis applied to genomic data can provide a powerful tool to distinguish milk composition QTL.					
31178181	2	4	theme	composition	417:427	arg1	traits					429:434	milk composition traits	412:434	milk composition traits	412:434	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	4	5	theme	milk	985:988	arg1	composition					990:1000	milk composition	985:1000	milk composition	985:1000	The PLS detected most of the QTL identified using single-trait GWAS, usually with a higher significance value, as well as previously undetected QTL for milk composition.					
31178181	4	6	theme	higher	917:922	arg1	value					937:941	a higher significance value	915:941	a higher significance value	915:941	The PLS detected most of the QTL identified using single-trait GWAS, usually with a higher significance value, as well as previously undetected QTL for milk composition.					
31178181	3	7	from	GWAS	647:650	arg1	wavenumbers					670:680	individual MIR wavenumbers	655:680	individual MIR wavenumbers	655:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	5	8	theme	increased	1103:1111	arg1	power					1113:1117	the increased power	1099:1117	the increased power	1099:1117	Each QTL tends to have a different pattern of effects across the MIR spectrum and this explains the increased power.					
31178181	3	9	theme	least	697:701	arg1	squares					703:709	a partial least squares	687:709	a partial least squares regression (PLS)	687:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	2	10	from	data	404:407	arg1	cows					541:544	crossbred cows	531:544	crossbred cows	531:544	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	2	10	from	data	404:407	arg1	Jersey					519:524	Jersey	519:524	Jersey	519:524	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	2	10	from	data	404:407	arg1	Holstein					509:516	Holstein	509:516	Holstein	509:516	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	3	11	from	wavenumbers	670:680	arg1	protein					583:589	protein	583:589	protein	583:589	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	11	from	wavenumbers	670:680	arg1	concentrations					621:634	fatty acid concentrations	610:634	fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS)	610:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	11	from	wavenumbers	670:680	arg1	fat					601:603	fat	601:603	fat	601:603	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	11	from	wavenumbers	670:680	arg1	lactose					592:598	lactose	592:598	lactose	592:598	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	4	12	theme	undetected	966:975	arg1	QTL					977:979	previously undetected QTL	955:979	previously undetected QTL for milk composition	955:1000	The PLS detected most of the QTL identified using single-trait GWAS, usually with a higher significance value, as well as previously undetected QTL for milk composition.					
31178181	3	13	theme	squares	703:709	arg1	PLS					723:725	PLS	723:725	PLS	723:725	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	13	theme	squares	703:709	arg1	regression					711:720	a partial least squares regression	687:720	a partial least squares regression (PLS)	687:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	6	14	theme	effect	1190:1195	arg1	patterns					1178:1185	different patterns	1168:1185	different patterns of effect	1168:1195	Because SNP tracking different QTL tend to have different patterns of effect, it was possible to distinguish closely linked QTL.					
31178181	1	15	theme	linked	321:326	arg1	QTL					353:355	QTL	353:355	QTL	353:355	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	1	15	theme	linked	321:326	arg1	loci					347:350	linked quantitative trait loci	321:350	linked quantitative trait loci (QTL)	321:356	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	3	16	from	milk	639:642	arg1	wavenumbers					670:680	individual MIR wavenumbers	655:680	individual MIR wavenumbers	655:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	4	17	dep	usually	902:908	arg1	with					910:913	with	910:913	with	910:913	The PLS detected most of the QTL identified using single-trait GWAS, usually with a higher significance value, as well as previously undetected QTL for milk composition.					
31178181	1	18	theme	mid-infrared	139:150	arg1	analysis					171:178	mid-infrared (MIR) spectroscopy analysis	139:178	mid-infrared (MIR) spectroscopy analysis of milk samples	139:194	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	2	19	theme	MIR	445:447	arg1	spectra					449:455	related MIR spectra	437:455	related MIR spectra	437:455	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	1	20	theme	quantitative	328:339	arg1	QTL					353:355	QTL	353:355	QTL	353:355	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	1	20	theme	quantitative	328:339	arg1	loci					347:350	linked quantitative trait loci	321:350	linked quantitative trait loci (QTL)	321:356	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	5	21	theme	MIR	1068:1070	arg1	spectrum					1072:1079	the MIR spectrum	1064:1079	the MIR spectrum	1064:1079	Each QTL tends to have a different pattern of effects across the MIR spectrum and this explains the increased power.					
31178181	3	22	theme	multi-trait	754:764	arg1	GWAS					766:769	a multi-trait GWAS	752:769	a multi-trait GWAS	752:769	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	4	23	theme	single-trait	883:894	arg1	GWAS					896:899	single-trait GWAS	883:899	single-trait GWAS	883:899	The PLS detected most of the QTL identified using single-trait GWAS, usually with a higher significance value, as well as previously undetected QTL for milk composition.					
31178181	1	24	theme	trait	341:345	arg1	QTL					353:355	QTL	353:355	QTL	353:355	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	1	24	theme	trait	341:345	arg1	loci					347:350	linked quantitative trait loci	321:350	linked quantitative trait loci (QTL)	321:356	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	2	25	theme	related	437:443	arg1	spectra					449:455	related MIR spectra	437:455	related MIR spectra	437:455	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	6	26	theme	tracking	1132:1139	arg1	QTL					1151:1153	SNP tracking different QTL	1128:1153	SNP tracking different QTL	1128:1153	Because SNP tracking different QTL tend to have different patterns of effect, it was possible to distinguish closely linked QTL.					
31178181	1	27	link	linked	321:326	arg1	QTL					353:355	QTL	353:355	QTL	353:355	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	1	27	link	linked	321:326	arg1	loci					347:350	linked quantitative trait loci	321:350	linked quantitative trait loci (QTL)	321:356	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	1	28	theme	MIR	153:155	arg1	analysis					171:178	mid-infrared (MIR) spectroscopy analysis	139:178	mid-infrared (MIR) spectroscopy analysis of milk samples	139:194	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	3	29	from	GWAS	575:578	arg1	protein					583:589	protein	583:589	protein	583:589	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	29	from	GWAS	575:578	arg1	concentrations					621:634	fatty acid concentrations	610:634	fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS)	610:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	29	from	GWAS	575:578	arg1	fat					601:603	fat	601:603	fat	601:603	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	29	from	GWAS	575:578	arg1	lactose					592:598	lactose	592:598	lactose	592:598	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	1	30	theme	genome-wide	235:245	arg1	studies					259:265	genome-wide association studies	235:265	genome-wide association studies (GWAS)	235:272	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	1	30	theme	genome-wide	235:245	arg1	GWAS					268:271	GWAS	268:271	GWAS	268:271	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	2	31	theme	crossbred	531:539	arg1	cows					541:544	crossbred cows	531:544	crossbred cows	531:544	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	7	32	theme	milk	1415:1418	arg1	QTL					1432:1434	milk composition QTL	1415:1434	milk composition QTL	1415:1434	Overall, the results of this study suggest that using MIR data through either GWAS or PLS analysis applied to genomic data can provide a powerful tool to distinguish milk composition QTL.					
31178181	0	33	theme	mid-infrared	11:22	arg1	spectra					24:30	mid-infrared spectra	11:30	mid-infrared spectra	11:30	The use of mid-infrared spectra to map genes affecting milk composition.					
31178181	1	34	theme	association	247:257	arg1	studies					259:265	genome-wide association studies	235:265	genome-wide association studies (GWAS)	235:272	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	1	34	theme	association	247:257	arg1	GWAS					268:271	GWAS	268:271	GWAS	268:271	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	2	35	theme	626,777	488:494	arg1	SNP					496:498	626,777 SNP	488:498	626,777 SNP	488:498	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	7	36	theme	genomic	1359:1365	arg1	data					1367:1370	genomic data	1359:1370	genomic data	1359:1370	Overall, the results of this study suggest that using MIR data through either GWAS or PLS analysis applied to genomic data can provide a powerful tool to distinguish milk composition QTL.					
31178181	1	37	theme	spectroscopy	158:169	arg1	analysis					171:178	mid-infrared (MIR) spectroscopy analysis	139:178	mid-infrared (MIR) spectroscopy analysis of milk samples	139:194	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	1	38	theme	studies	259:265	arg1	precision					222:230	precision	222:230	precision	222:230	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	1	38	theme	studies	259:265	arg1	power					212:216	the power	208:216	the power	208:216	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	6	39	theme	different	1168:1176	arg1	patterns					1178:1185	different patterns	1168:1185	different patterns of effect	1168:1195	Because SNP tracking different QTL tend to have different patterns of effect, it was possible to distinguish closely linked QTL.					
31178181	3	40	theme	conventional	562:573	arg1	equivalent					738:747	equivalent	738:747	equivalent	738:747	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	40	theme	conventional	562:573	arg1	GWAS					575:578	a conventional GWAS	560:578	a conventional GWAS	560:578	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	41	theme	MIR	783:785	arg1	data					787:790	MIR data	783:790	MIR data	783:790	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	2	42	theme	milk	412:415	arg1	traits					429:434	milk composition traits	412:434	milk composition traits	412:434	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	5	43	theme	different	1028:1036	arg1	pattern					1038:1044	a different pattern	1026:1044	a different pattern of effects	1026:1055	Each QTL tends to have a different pattern of effects across the MIR spectrum and this explains the increased power.					
31178181	3	44	from	lactose	592:598	arg1	milk					639:642	milk	639:642	milk	639:642	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	44	from	lactose	592:598	arg1	GWAS					647:650	a GWAS	645:650	a GWAS on individual MIR wavenumbers	645:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	44	from	lactose	592:598	arg1	regression					711:720	a partial least squares regression	687:720	a partial least squares regression (PLS)	687:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	44	from	lactose	592:598	arg1	PLS					723:725	PLS	723:725	PLS	723:725	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	44	from	lactose	592:598	arg1	wavenumbers					670:680	individual MIR wavenumbers	655:680	individual MIR wavenumbers	655:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	45	from	fat	601:603	arg1	milk					639:642	milk	639:642	milk	639:642	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	45	from	fat	601:603	arg1	GWAS					647:650	a GWAS	645:650	a GWAS on individual MIR wavenumbers	645:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	45	from	fat	601:603	arg1	regression					711:720	a partial least squares regression	687:720	a partial least squares regression (PLS)	687:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	45	from	fat	601:603	arg1	PLS					723:725	PLS	723:725	PLS	723:725	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	45	from	fat	601:603	arg1	wavenumbers					670:680	individual MIR wavenumbers	655:680	individual MIR wavenumbers	655:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	5	46	contain	have	1021:1024	arg1	QTL					1008:1010	Each QTL	1003:1010	Each QTL	1003:1010	Each QTL tends to have a different pattern of effects across the MIR spectrum and this explains the increased power.					
31178181	5	46	contain	have	1021:1024	arg2	pattern					1038:1044	a different pattern	1026:1044	a different pattern of effects	1026:1055	Each QTL tends to have a different pattern of effects across the MIR spectrum and this explains the increased power.					
31178181	0	47	theme	spectra	24:30	arg1	use					4:6	The use	0:6	The use of mid-infrared spectra	0:30	The use of mid-infrared spectra to map genes affecting milk composition.					
31178181	3	48	theme	MIR	666:668	arg1	wavenumbers					670:680	individual MIR wavenumbers	655:680	individual MIR wavenumbers	655:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	2	49	theme	genotypic	462:470	arg1	data					472:475	genotypic data	462:475	genotypic data comprising 626,777 SNP	462:498	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	7	50	theme	PLS	1335:1337	arg1	analysis					1339:1346	PLS analysis	1335:1346	PLS analysis	1335:1346	Overall, the results of this study suggest that using MIR data through either GWAS or PLS analysis applied to genomic data can provide a powerful tool to distinguish milk composition QTL.					
31178181	3	51	from	concentrations	621:634	arg1	milk					639:642	milk	639:642	milk	639:642	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	51	from	concentrations	621:634	arg1	GWAS					647:650	a GWAS	645:650	a GWAS on individual MIR wavenumbers	645:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	51	from	concentrations	621:634	arg1	regression					711:720	a partial least squares regression	687:720	a partial least squares regression (PLS)	687:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	51	from	concentrations	621:634	arg1	PLS					723:725	PLS	723:725	PLS	723:725	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	51	from	concentrations	621:634	arg1	wavenumbers					670:680	individual MIR wavenumbers	655:680	individual MIR wavenumbers	655:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	1	52	theme	milk	183:186	arg1	samples					188:194	milk samples	183:194	milk samples	183:194	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	6	53	theme	linked	1237:1242	arg1	QTL					1244:1246	closely linked QTL	1229:1246	closely linked QTL	1229:1246	Because SNP tracking different QTL tend to have different patterns of effect, it was possible to distinguish closely linked QTL.					
31178181	7	54	theme	composition	1420:1430	arg1	QTL					1432:1434	milk composition QTL	1415:1434	milk composition QTL	1415:1434	Overall, the results of this study suggest that using MIR data through either GWAS or PLS analysis applied to genomic data can provide a powerful tool to distinguish milk composition QTL.					
31178181	3	55	theme	fatty	610:614	arg1	concentrations					621:634	fatty acid concentrations	610:634	fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS)	610:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	1	56	theme	study	89:93	arg1	aim					77:79	The aim	73:79	The aim of this study	73:93	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	2	57	theme	phenotypic	393:402	arg1	data					404:407	phenotypic data	393:407	phenotypic data of milk composition traits	393:434	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	3	58	from	regression	711:720	arg1	wavenumbers					670:680	individual MIR wavenumbers	655:680	individual MIR wavenumbers	655:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	2	59	from	spectra	449:455	arg1	cows					541:544	crossbred cows	531:544	crossbred cows	531:544	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	2	59	from	spectra	449:455	arg1	Jersey					519:524	Jersey	519:524	Jersey	519:524	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	2	59	from	spectra	449:455	arg1	Holstein					509:516	Holstein	509:516	Holstein	509:516	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	1	60	theme	milk	278:281	arg1	composition					283:293	milk composition	278:293	milk composition	278:293	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	6	61	theme	different	1141:1149	arg1	QTL					1151:1153	SNP tracking different QTL	1128:1153	SNP tracking different QTL	1128:1153	Because SNP tracking different QTL tend to have different patterns of effect, it was possible to distinguish closely linked QTL.					
31178181	5	62	theme	effects	1049:1055	arg1	pattern					1038:1044	a different pattern	1026:1044	a different pattern of effects	1026:1055	Each QTL tends to have a different pattern of effects across the MIR spectrum and this explains the increased power.					
31178181	3	63	theme	SNP	818:820	arg1	genotypes					822:830	SNP genotypes	818:830	SNP genotypes	818:830	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	4	64	theme	significance	924:935	arg1	value					937:941	a higher significance value	915:941	a higher significance value	915:941	The PLS detected most of the QTL identified using single-trait GWAS, usually with a higher significance value, as well as previously undetected QTL for milk composition.					
31178181	1	65	theme	samples	188:194	arg1	analysis					171:178	mid-infrared (MIR) spectroscopy analysis	139:178	mid-infrared (MIR) spectroscopy analysis of milk samples	139:194	The aim of this study was to investigate the feasibility of using mid-infrared (MIR) spectroscopy analysis of milk samples to increase the power and precision of genome-wide association studies (GWAS) for milk composition and to better distinguish linked quantitative trait loci (QTL).					
31178181	3	66	theme	individual	655:664	arg1	wavenumbers					670:680	individual MIR wavenumbers	655:680	individual MIR wavenumbers	655:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	6	67	link	linked	1237:1242	arg1	QTL					1244:1246	closely linked QTL	1229:1246	closely linked QTL	1229:1246	Because SNP tracking different QTL tend to have different patterns of effect, it was possible to distinguish closely linked QTL.					
31178181	3	68	theme	partial	689:695	arg1	squares					703:709	a partial least squares	687:709	a partial least squares regression (PLS)	687:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	0	69	theme	milk	55:58	arg1	composition					60:70	milk composition	55:70	milk composition	55:70	The use of mid-infrared spectra to map genes affecting milk composition.					
31178181	6	70	contain	have	1163:1166	arg1	QTL					1151:1153	SNP tracking different QTL	1128:1153	SNP tracking different QTL	1128:1153	Because SNP tracking different QTL tend to have different patterns of effect, it was possible to distinguish closely linked QTL.					
31178181	6	70	contain	have	1163:1166	arg2	patterns					1178:1185	different patterns	1168:1185	different patterns of effect	1168:1195	Because SNP tracking different QTL tend to have different patterns of effect, it was possible to distinguish closely linked QTL.					
31178181	3	71	from	protein	583:589	arg1	milk					639:642	milk	639:642	milk	639:642	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	71	from	protein	583:589	arg1	GWAS					647:650	a GWAS	645:650	a GWAS on individual MIR wavenumbers	645:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	71	from	protein	583:589	arg1	regression					711:720	a partial least squares regression	687:720	a partial least squares regression (PLS)	687:726	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	71	from	protein	583:589	arg1	PLS					723:725	PLS	723:725	PLS	723:725	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	3	71	from	protein	583:589	arg1	wavenumbers					670:680	individual MIR wavenumbers	655:680	individual MIR wavenumbers	655:680	We performed a conventional GWAS on protein, lactose, fat, and fatty acid concentrations in milk, a GWAS on individual MIR wavenumbers, and a partial least squares regression (PLS), which is equivalent to a multi-trait GWAS, exploiting MIR data simultaneously to predict SNP genotypes.					
31178181	7	72	theme	MIR	1303:1305	arg1	data					1307:1310	MIR data	1303:1310	MIR data	1303:1310	Overall, the results of this study suggest that using MIR data through either GWAS or PLS analysis applied to genomic data can provide a powerful tool to distinguish milk composition QTL.					
31178181	2	73	from	data	472:475	arg1	cows					541:544	crossbred cows	531:544	crossbred cows	531:544	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	2	73	from	data	472:475	arg1	Jersey					519:524	Jersey	519:524	Jersey	519:524	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	2	73	from	data	472:475	arg1	Holstein					509:516	Holstein	509:516	Holstein	509:516	To achieve this goal, we analyzed phenotypic data of milk composition traits, related MIR spectra, and genotypic data comprising 626,777 SNP on 5,202 Holstein, Jersey, and crossbred cows.					
31178181	7	74	theme	powerful	1386:1393	arg1	tool					1395:1398	a powerful tool	1384:1398	a powerful tool to distinguish milk composition QTL	1384:1434	Overall, the results of this study suggest that using MIR data through either GWAS or PLS analysis applied to genomic data can provide a powerful tool to distinguish milk composition QTL.					
31438595	2	0	theme	chromatography-diode	462:481	arg1	spectrometry					510:521	liquid chromatography-diode array detector-tandem mass spectrometry	455:521	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	0	theme	chromatography-diode	462:481	arg1	MS/MS					534:538	UHPLC-DAD MS/MS	524:538	UHPLC-DAD MS/MS	524:538	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	9	1	theme	promising	1451:1459	arg1	one					1461:1463	a promising one	1449:1463	a promising one for producing functional food	1449:1493	Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.					
31438595	2	2	theme	anion-exchange	317:330	arg1	HPAEC					348:352	HPAEC	348:352	HPAEC	348:352	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	2	theme	anion-exchange	317:330	arg1	chromatography					332:345	high-performance anion-exchange chromatography	300:345	high-performance anion-exchange chromatography (HPAEC)	300:353	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	6	3	theme	principal	915:923	arg1	analysis					935:942	the principal component analysis	911:942	the principal component analysis (PCA)	911:948	According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples.					
31438595	6	3	theme	principal	915:923	arg1	PCA					945:947	PCA	945:947	PCA	945:947	According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples.					
31438595	2	4	theme	liquid	455:460	arg1	spectrometry					510:521	liquid chromatography-diode array detector-tandem mass spectrometry	455:521	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	4	theme	liquid	455:460	arg1	MS/MS					534:538	UHPLC-DAD MS/MS	524:538	UHPLC-DAD MS/MS	524:538	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	5	5	dep	acid	788:791	arg1	mg/kg					806:810	up to 59.93 mg/kg	794:810	up to 59.93 mg/kg in clone III/9	794:825	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	2	6	theme	high-performance	300:315	arg1	HPAEC					348:352	HPAEC	348:352	HPAEC	348:352	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	6	theme	high-performance	300:315	arg1	chromatography					332:345	high-performance anion-exchange chromatography	300:345	high-performance anion-exchange chromatography (HPAEC)	300:353	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	7	theme	UHPLC-DAD	524:532	arg1	spectrometry					510:521	liquid chromatography-diode array detector-tandem mass spectrometry	455:521	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	7	theme	UHPLC-DAD	524:532	arg1	MS/MS					534:538	UHPLC-DAD MS/MS	524:538	UHPLC-DAD MS/MS	524:538	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	5	8	with	polyphenol	712:721	arg1	acid					843:846	p-coumaric acid	832:846	p-coumaric acid (up to 53.99 mg/kg in clone VIII/1)	832:882	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	5	8	with	polyphenol	712:721	arg1	acid					788:791	chlorogenic acid	776:791	chlorogenic acid (up to 59.93 mg/kg in clone III/9)	776:826	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	6	9	theme	pollen	1052:1057	arg1	samples					1059:1065	separating pollen samples	1041:1065	separating pollen samples	1041:1065	According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples.					
31438595	5	10	dep	53.99	855:859	arg1	to					852:853	to	852:853	to	852:853	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	5	11	theme	p-coumaric	832:841	arg1	acid					843:846	p-coumaric acid	832:846	p-coumaric acid (up to 53.99 mg/kg in clone VIII/1)	832:882	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	2	12	theme	spectrometry	510:521	arg1	system					541:546	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	9	13	theme	functional	1479:1488	arg1	food					1490:1493	functional food	1479:1493	functional food	1479:1493	Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.					
31438595	8	14	theme	chemical	1214:1221	arg1	composition					1223:1233	chemical composition	1214:1233	chemical composition of studied 'Oblačinska sour cherry' clone pollen	1214:1282	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population.					
31438595	4	15	theme	stress	651:656	arg1	sugars					658:663	stress sugars	651:663	stress sugars	651:663	Some samples had high level of stress sugars, especially trehalose.					
31438595	4	15	theme	stress	651:656	arg1	trehalose					677:685	trehalose	677:685	especially trehalose	666:685	Some samples had high level of stress sugars, especially trehalose.					
31438595	2	16	theme	pulsed	360:365	arg1	detection					380:388	pulsed amperometric detection	360:388	pulsed amperometric detection (PAD)	360:394	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	16	theme	pulsed	360:365	arg1	PAD					391:393	PAD	391:393	PAD	391:393	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	17	theme	mass	505:508	arg1	spectrometry					510:521	liquid chromatography-diode array detector-tandem mass spectrometry	455:521	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	17	theme	mass	505:508	arg1	MS/MS					534:538	UHPLC-DAD MS/MS	524:538	UHPLC-DAD MS/MS	524:538	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	0	18	dep	Pollen	68:73	arg1	Fingerprint					9:19	Chemical Fingerprint	0:19	Chemical Fingerprint of 'Oblačinska' Sour Cherry (Prunuscerasus L.)	0:66	Chemical Fingerprint of 'Oblačinska' Sour Cherry (Prunuscerasus L.) Pollen.					
31438595	4	19	theme	high	637:640	arg1	level					642:646	high level	637:646	high level of stress sugars, especially trehalose	637:685	Some samples had high level of stress sugars, especially trehalose.					
31438595	9	20	theme	highest	1367:1373	arg1	level					1375:1379	the highest level	1363:1379	the highest level of phenolics	1363:1392	Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.					
31438595	7	21	theme	phenolics	1158:1166	arg1	quantity					1146:1153	the quantity	1142:1153	the quantity of phenolics and dissimilar profiles	1142:1190	PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles.					
31438595	8	22	from	differences	1199:1209	arg1	composition					1223:1233	chemical composition	1214:1233	chemical composition of studied 'Oblačinska sour cherry' clone pollen	1214:1282	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population.					
31438595	2	23	theme	Carbohydrate	263:274	arg1	analysis					276:283	Carbohydrate analysis	263:283	Carbohydrate analysis	263:283	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	1	24	theme	pollen	140:145	arg1	phenolics					127:135	phenolics	127:135	phenolics	127:135	The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar.					
31438595	1	24	theme	pollen	140:145	arg1	sugars					116:121	sugars	116:121	sugars	116:121	The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar.					
31438595	0	25	theme	Chemical	0:7	arg1	Fingerprint					9:19	Chemical Fingerprint	0:19	Chemical Fingerprint of 'Oblačinska' Sour Cherry (Prunuscerasus L.)	0:66	Chemical Fingerprint of 'Oblačinska' Sour Cherry (Prunuscerasus L.) Pollen.					
31438595	5	26	dep	acid	843:846	arg1	mg/kg					861:865	up to 53.99 mg/kg	849:865	up to 53.99 mg/kg in clone VIII/1	849:881	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	8	27	theme	studied	1238:1244	arg1	pollen					1277:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population.					
31438595	9	28	theme	phenolics	1384:1392	arg1	level					1375:1379	the highest level	1363:1379	the highest level of phenolics	1363:1392	Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.					
31438595	5	29	theme	chlorogenic	776:786	arg1	acid					788:791	chlorogenic acid	776:791	chlorogenic acid (up to 59.93 mg/kg in clone III/9)	776:826	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	1	30	theme	chemical	224:231	arg1	fingerprint					233:243	the chemical fingerprint	220:243	the chemical fingerprint of this cultivar	220:260	The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar.					
31438595	9	31	theme	medicinal	1505:1513	arg1	treatments					1515:1524	medicinal treatments	1505:1524	medicinal treatments	1505:1524	Finally, due to the highest level of phenolics, clones IV/8, XV/3, and VIII/1 could be singled out as a promising one for producing functional food and/or in medicinal treatments.					
31438595	5	32	theme	clone	870:874	arg1	VIII/1					876:881	clone VIII/1	870:881	clone VIII/1	870:881	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	6	33	from	sugars	1031:1036	arg1	samples					1059:1065	separating pollen samples	1041:1065	separating pollen samples	1041:1065	According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples.					
31438595	7	34	theme	dissimilar	1172:1181	arg1	profiles					1183:1190	dissimilar profiles	1172:1190	dissimilar profiles	1172:1190	PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles.					
31438595	6	35	theme	separating	1041:1050	arg1	samples					1059:1065	separating pollen samples	1041:1065	separating pollen samples	1041:1065	According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples.					
31438595	5	36	dep	mg/kg	750:754	arg1	up					737:738	up	737:738	up	737:738	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	8	37	theme	sour	1258:1261	arg1	pollen					1277:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population.					
31438595	7	38	theme	profiles	1183:1190	arg1	quantity					1146:1153	the quantity	1142:1153	the quantity of phenolics and dissimilar profiles	1142:1190	PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles.					
31438595	2	39	theme	ultra-high-performance	432:453	arg1	system					541:546	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	4	40	contain	had	633:635	arg1	samples					625:631	Some samples	620:631	Some samples	620:631	Some samples had high level of stress sugars, especially trehalose.					
31438595	4	40	contain	had	633:635	arg2	level					642:646	high level	637:646	high level of stress sugars, especially trehalose	637:685	Some samples had high level of stress sugars, especially trehalose.					
31438595	8	41	theme	clone	1271:1275	arg1	pollen					1277:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population.					
31438595	1	42	theme	different	164:172	arg1	clones					199:204	15 different 'Oblačinska' sour cherry clones	161:204	15 different 'Oblačinska' sour cherry clones	161:204	The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar.					
31438595	0	43	theme	Oblačinska	25:34	arg1	Cherry					42:47	'Oblačinska' Sour Cherry	24:47	'Oblačinska' Sour Cherry	24:47	Chemical Fingerprint of 'Oblačinska' Sour Cherry (Prunuscerasus L.) Pollen.					
31438595	5	44	theme	clone	757:761	arg1	III/9					763:767	clone III/9	757:767	clone III/9	757:767	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	5	44	theme	clone	757:761	arg1	mg/kg					750:754	181.12 mg/kg	743:754	181.12 mg/kg (clone III/9)	743:768	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	5	45	from	polyphenol	712:721	arg1	quantity					728:735	a quantity	726:735	a quantity up to 181.12 mg/kg (clone III/9)	726:768	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	2	46	theme	amperometric	367:378	arg1	detection					380:388	pulsed amperometric detection	360:388	pulsed amperometric detection (PAD)	360:394	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	46	theme	amperometric	367:378	arg1	PAD					391:393	PAD	391:393	PAD	391:393	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	0	47	theme	Cherry	42:47	arg1	Fingerprint					9:19	Chemical Fingerprint	0:19	Chemical Fingerprint of 'Oblačinska' Sour Cherry (Prunuscerasus L.)	0:66	Chemical Fingerprint of 'Oblačinska' Sour Cherry (Prunuscerasus L.) Pollen.					
31438595	1	48	theme	cultivar	253:260	arg1	fingerprint					233:243	the chemical fingerprint	220:243	the chemical fingerprint of this cultivar	220:260	The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar.					
31438595	8	49	theme	Oblačinska	1247:1256	arg1	pollen					1277:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population.					
31438595	3	50	theme	abundant	570:577	arg1	sugar					579:583	the most abundant sugar	561:583	the most abundant sugar	561:583	Glucose was the most abundant sugar, followed by fructose and sucrose.					
31438595	3	50	theme	abundant	570:577	arg1	Glucose					549:555	Glucose	549:555	Glucose	549:555	Glucose was the most abundant sugar, followed by fructose and sucrose.					
31438595	6	51	theme	important	1021:1029	arg1	sugars					1031:1036	the most important sugars	1012:1036	the most important sugars in separating pollen samples	1012:1065	According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples.					
31438595	0	52	theme	Sour	37:40	arg1	Cherry					42:47	'Oblačinska' Sour Cherry	24:47	'Oblačinska' Sour Cherry	24:47	Chemical Fingerprint of 'Oblačinska' Sour Cherry (Prunuscerasus L.) Pollen.					
31438595	5	53	theme	clone	815:819	arg1	III/9					821:825	clone III/9	815:825	clone III/9	815:825	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	8	54	theme	pollen	1277:1282	arg1	composition					1223:1233	chemical composition	1214:1233	chemical composition of studied 'Oblačinska sour cherry' clone pollen	1214:1282	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population.					
31438595	7	55	dep	clones	1093:1098	arg1	clones					1093:1098	clones VIII/1, IV/8, III/9, and V/P	1093:1127	clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles	1093:1190	PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles.					
31438595	7	55	dep	clones	1093:1098	arg1	VIII/1					1100:1105	VIII/1	1100:1105	VIII/1	1100:1105	PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles.					
31438595	7	55	dep	clones	1093:1098	arg1	V/P					1125:1127	V/P	1125:1127	V/P	1125:1127	PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles.					
31438595	7	55	dep	clones	1093:1098	arg1	IV/8					1108:1111	IV/8	1108:1111	IV/8	1108:1111	PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles.					
31438595	7	55	dep	clones	1093:1098	arg1	III/9					1114:1118	III/9	1114:1118	III/9	1114:1118	PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles.					
31438595	7	55	dep	clones	1093:1098	arg1	off					1089:1091	off	1089:1091	off	1089:1091	PCA showed splitting off clones VIII/1, IV/8, III/9, and V/P according to the quantity of phenolics and dissimilar profiles.					
31438595	1	56	theme	research	92:99	arg1	aim					80:82	The aim	76:82	The aim of this research	76:99	The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar.					
31438595	8	57	theme	cherry	1263:1268	arg1	pollen					1277:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	studied 'Oblačinska sour cherry' clone pollen	1238:1282	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population.					
31438595	1	58	dep	clones	199:204	arg1	Oblačinska					175:184	Oblačinska	175:184	Oblačinska	175:184	The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar.					
31438595	5	59	dep	59.93	800:804	arg1	to					797:798	to	797:798	to	797:798	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	5	60	from	mg/kg	861:865	arg1	VIII/1					876:881	clone VIII/1	870:881	clone VIII/1	870:881	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	2	61	theme	detector-tandem	489:503	arg1	spectrometry					510:521	liquid chromatography-diode array detector-tandem mass spectrometry	455:521	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	61	theme	detector-tandem	489:503	arg1	MS/MS					534:538	UHPLC-DAD MS/MS	524:538	UHPLC-DAD MS/MS	524:538	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	0	62	dep	Fingerprint	9:19	arg1	L.					64:65	L.	64:65	L.	64:65	Chemical Fingerprint of 'Oblačinska' Sour Cherry (Prunuscerasus L.) Pollen.					
31438595	8	63	theme	Large	1193:1197	arg1	differences					1199:1209	Large differences	1193:1209	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen	1193:1282	Large differences in chemical composition of studied 'Oblačinska sour cherry' clone pollen were shown, proving that it is not a cultivar, but population.					
31438595	1	64	theme	sour	187:190	arg1	clones					199:204	15 different 'Oblačinska' sour cherry clones	161:204	15 different 'Oblačinska' sour cherry clones	161:204	The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar.					
31438595	2	65	theme	array	483:487	arg1	spectrometry					510:521	liquid chromatography-diode array detector-tandem mass spectrometry	455:521	ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system	432:546	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	2	65	theme	array	483:487	arg1	MS/MS					534:538	UHPLC-DAD MS/MS	524:538	UHPLC-DAD MS/MS	524:538	Carbohydrate analysis was done using high-performance anion-exchange chromatography (HPAEC) with pulsed amperometric detection (PAD), while polyphenols were analyzed by ultra-high-performance liquid chromatography-diode array detector-tandem mass spectrometry (UHPLC-DAD MS/MS) system.					
31438595	6	66	theme	component	925:933	arg1	analysis					935:942	the principal component analysis	911:942	the principal component analysis (PCA)	911:948	According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples.					
31438595	6	66	theme	component	925:933	arg1	PCA					945:947	PCA	945:947	PCA	945:947	According to the principal component analysis (PCA), fructose, maltose, maltotriose, sorbitol, and trehalose were the most important sugars in separating pollen samples.					
31438595	5	67	from	mg/kg	806:810	arg1	III/9					821:825	clone III/9	815:825	clone III/9	815:825	Rutin was predominantly polyphenol in a quantity up to 181.12 mg/kg (clone III/9), with chlorogenic acid (up to 59.93 mg/kg in clone III/9) and p-coumaric acid (up to 53.99 mg/kg in clone VIII/1) coming after.					
31438595	4	68	theme	sugars	658:663	arg1	level					642:646	high level	637:646	high level of stress sugars, especially trehalose	637:685	Some samples had high level of stress sugars, especially trehalose.					
31438595	1	69	theme	cherry	192:197	arg1	clones					199:204	15 different 'Oblačinska' sour cherry clones	161:204	15 different 'Oblačinska' sour cherry clones	161:204	The aim of this research was to analyze sugars and phenolics of pollen obtained from 15 different 'Oblačinska' sour cherry clones and to assess the chemical fingerprint of this cultivar.